{ "genome": { "genus": "Ureaplasma", "species": "parvum", "strain": "SV3F4", "taxon": "Ureaplasma parvum SV3F4", "complete": true, "gram": "?", "translation_table": 11 }, "stats": { "size": 727289, "gc": 0.25545965909012786, "n_ratio": 0.0, "n50": 727289, "n90": 727289, "coding_ratio": 0.7077956630720388 }, "features": [ { "type": "cds", "sequence": "contig_1", "start": 163, "stop": 492, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTAGTTATTCCTTTTGGATTTGGAATTACACTTTTACGAATTTTCATTATTTCTAAATTATTAAATAAGCGTTTAAAAATGAATAATTTAGGTGTCAATCCCAAAAAATATCGTCGATTACCAGCAAATTTTTTTGCTTTTGGCACTATTTACATTTTATTTAATTTTCTAATATTAGTACCTTTGATCATTGGTAATCTTGTTAACCTTTATCAATCTATTTTTGAGACCATCTCATTATTTGTTTTAACATTTTTTATGTTTTTTATCCAAATGGGGATGCAAATTTATTTAGGAATTAAAAAAGATAAAGGAGAAAAAAAATAA", "aa": "MLVIPFGFGITLLRIFIISKLLNKRLKMNNLGVNPKKYRRLPANFFAFGTIYILFNFLILVPLIIGNLVNLYQSIFETISLFVLTFFMFFIQMGMQIYLGIKKDKGEKK", "aa_hexdigest": "4024215d580af1aa2aa8e8141e9e2a9c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12733.483599999989, "isoelectric_point": 10.383745384216308 }, "id": "NKIECMJPJD_42", "locus": "GCA000828735_00001" }, { "type": "cds", "sequence": "contig_1", "start": 518, "stop": 1012, "strand": "+", "frame": 2, "gene": "atpB", "product": "F0F1 ATP synthase subunit A", "db_xrefs": [ "COG:C", "COG:COG0356", "EC:7.1.2.2", "KEGG:K02108", "SO:0001217", "UniRef:UniRef50_Q9PR07", "UniRef:UniRef90_Q9PR07" ], "nt": "GTGGAAAATTATAGCCCGCTTGATATAATGATTGCACTTCCACATATTGCTGCAATCATTATGGTAACTTTAATTATTGGAACAGTTTCGTTGATTTATTTTAGTATGGTTAAAAAGTTAACTGTACATGATGTGCCAAACCGTTTTGTAATTATTATTGGAATGATTGTTGATTATTTTAGAGGACTAGTTGTTGATACAATGGGCGCTAAACATGTTAAATTAGCACCTTATGTACTATTTACATTTTGTTATATTTTTACAGCTAACTTAGTTTCTTTATTTGGTTTCAAAGAAGCAACAACTGCATCTGCCGTTCCATTAGCAATGGCTCTTGGAACCGTGATCGGTGGTCAAATTGTTGCCTTAAAATACCAAAAAGCTTCATTCTTTTTAAAATTTGCTTTTAAAATTAAAGGTTTTCCAATTATGATAAACCCTTTGGAAATCGTTTCTAAATTAACCCCTATTATTTCATTAACATTCCGTTTATGA", "aa": "MENYSPLDIMIALPHIAAIIMVTLIIGTVSLIYFSMVKKLTVHDVPNRFVIIIGMIVDYFRGLVVDTMGAKHVKLAPYVLFTFCYIFTANLVSLFGFKEATTASAVPLAMALGTVIGGQIVALKYQKASFFLKFAFKIKGFPIMINPLEIVSKLTPIISLTFRL", "aa_hexdigest": "b398aabdad273e57f5e9b2e2c6590aa3", "start_type": "GTG", "rbs_motif": "GGAGG", "genes": [ "atpB" ], "truncated": "3-prime", "pseudogene": { "start": 518, "stop": 1315, "observations": { "mutation": [ 1010, 1055, 1088, 1205 ], "stop": [ 1010, 1055, 1088, 1205 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PR07", "query_cov": 1.0, "subject_cov": 0.5963636363636363, "identity": 0.9329999999999999, "score": 295.0, "evalue": 6.1e-99, "gene_start": 1, "gene_end": 164, "reference_start": 1, "reference_end": 164, "reference_sequence": "MENYNPLDIMIALPHIAAIIIVTLIIATISLIYFSMIRKLTVHDVPNRFVIIIGMIVDYFRGLVVDTMGAKHVKLAPYVLFTFCYIFTANLVSLFGFKEATTASSVPLAMALATVVGGQIVALKYQKASFFLKFTFKIKGFPIMVNPLEIVSKLTPIISLTFRLWGNISAAAILLNITYWAFAGFTNVVPWVGVSLIAAVIILPILIGYFTCFAGTIQAFVFTLLTSINWGLEIKEGEEHYAHLAHKKAEKLAAKKLAELDAQNQAQNNEVQVVL" }, "paralog": false, "identity": 0.9283018867924528, "score": 478.0, "evalue": 7.38e-174, "description": "Internal stop codon at 1010, 1055, 1088, 1205. Nonsense mutation around 1010, 1055, 1088, 1205.", "psc": { "uniref90_id": "UniRef90_Q9PR07", "gene": "AtpB", "product": "F0F1 ATP synthase subunit A", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_Q9PR07", "cog_id": "COG0356", "cog_category": "C", "kegg_orthology_id": "K02108", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PR07", "EC:7.1.2.2", "UniRef:UniRef50_Q9PR07", "COG:COG0356", "COG:C", "KEGG:K02108" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PR07", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR07" ], "product": "ATP synthase A chain" } }, "id": "NKIECMJPJD_43", "locus": "GCA000828735_00002" }, { "type": "cds", "sequence": "contig_1", "start": 1394, "stop": 1723, "strand": "+", "frame": 2, "gene": "atpE", "product": "ATP synthase subunit c", "db_xrefs": [ "COG:C", "COG:COG0636", "GO:0005886", "GO:0008289", "GO:0045263", "GO:0046933", "RefSeq:WP_006688882.1", "SO:0001217", "UniParc:UPI00001265E0", "UniRef:UniRef100_Q9PR08", "UniRef:UniRef50_Q9PR08", "UniRef:UniRef90_Q9PR08" ], "nt": "ATGTCATCATTTATTGATATTACAAATGTTATTTCATCACATATTGAAGCTAATTTACCAGCAATTGCTTCTGAAAATGTAGGTTCACTTGCAAACGGTGCAGGAATTGCTTACTTAGGTAAATATATTGGTACAGGAATTACAATGTTAGCAGCTGGTGCAGTTGGTTTAATGCAAGGTTTTTCTACAGCTAATGCTGTACAAGCTGTAGCTCGTAACCCAGAAGCACAACCAAAGATTTTAAGTACAATGATTGTTGGTTTAGCATTGGCTGAAGCTGTTGCTATTTATGCACTTATTGTTTCTATCTTAATTATTTTCGTTGCTTAA", "aa": "MSSFIDITNVISSHIEANLPAIASENVGSLANGAGIAYLGKYIGTGITMLAAGAVGLMQGFSTANAVQAVARNPEAQPKILSTMIVGLALAEAVAIYALIVSILIIFVA", "aa_hexdigest": "abba82d7058f4834116cb1584b5ec605", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI00001265E0", "ncbi_nrp_id": "WP_006688882.1", "uniref100_id": "UniRef100_Q9PR08", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001265E0", "RefSeq:WP_006688882.1", "UniRef:UniRef100_Q9PR08" ] }, "ips": { "uniref100_id": "UniRef100_Q9PR08", "uniref90_id": "UniRef90_Q9PR08" }, "psc": { "uniref90_id": "UniRef90_Q9PR08", "gene": "atpE", "product": "ATP synthase subunit c", "uniref50_id": "UniRef50_Q9PR08", "cog_id": "COG0636", "cog_category": "C", "go_ids": [ "GO:0005886", "GO:0045263", "GO:0008289", "GO:0046933" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PR08", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR08" ], "product": "ATP synthase subunit c" }, "genes": [ "atpE" ], "id": "NKIECMJPJD_44", "locus": "GCA000828735_00003" }, { "type": "cds", "sequence": "contig_1", "start": 1792, "stop": 1938, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATTAAATAAAAAGCATTTAGTAGCTATATTATCCGTTCTTTCTTTATCAATTATAGTTGTACCACTGTTGACTTCATGTACTGGTGATATTCCAGAATTAAATCCAGCAGAGATTATTAATACACTATTCCCTAATGTATGA", "aa": "MKLNKKHLVAILSVLSLSIIVVPLLTSCTGDIPELNPAEIINTLFPNV", "aa_hexdigest": "85632efc15d7b7ddbf9b4c9e57b0ba7e", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5183.217799999999, "isoelectric_point": 6.511156654357909 }, "id": "NKIECMJPJD_45", "locus": "GCA000828735_00004" }, { "type": "cds", "sequence": "contig_1", "start": 2017, "stop": 2409, "strand": "+", "frame": 1, "gene": "atpF", "product": "F0F1 ATP synthase subunit B", "db_xrefs": [ "COG:C", "COG:COG0711", "EC:7.1.2.2", "GO:0005886", "GO:0015078", "GO:0015986", "GO:0045263", "GO:0046933", "GO:0046961", "SO:0001217", "UniParc:UPI000169D88C", "UniRef:UniRef100_Q9PR09", "UniRef:UniRef50_Q9PR09", "UniRef:UniRef90_Q9PR09" ], "nt": "ATGCTAGATAAAAGACGTGAATATATTGCTAAAGAAATTACAGATGCAGAAAATGCTAAACAAGAAGCTTTACAATATTTAGAAAACGCTAAATCTGAACATTTAGCAGCACAAGCACAAACGTTAGAAATTATTGCAAAAGCTAAAAGTGAATCATTAACTTTACGTGAATCATTAGAAAAAGAAGCACGTGAAGCTGCTGATAAAATTATTAGTTCTGCTAAAATTAGCATTGCTAATGAACGTAGAGAAAATCTTGAACGTTTACAATCTGAAGCTCGTGAAGCTGCTTATATTGCAGCTGAAGCATTGATGAAAAAAGAATTGTCTAGAGAAGATAATGATAAACTAGTTGATCAATTCATTAAAGAATTAGAAAATAATGAAAAATAA", "aa": "MLDKRREYIAKEITDAENAKQEALQYLENAKSEHLAAQAQTLEIIAKAKSESLTLRESLEKEAREAADKIISSAKISIANERRENLERLQSEAREAAYIAAEALMKKELSREDNDKLVDQFIKELENNEK", "aa_hexdigest": "40f8c0c9a2f0f568e69c1a277066cff8", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000169D88C", "uniref100_id": "UniRef100_Q9PR09", "db_xrefs": [ "SO:0001217", "UniParc:UPI000169D88C", "UniRef:UniRef100_Q9PR09" ] }, "ips": { "uniref100_id": "UniRef100_Q9PR09", "uniref90_id": "UniRef90_Q9PR09" }, "psc": { "uniref90_id": "UniRef90_Q9PR09", "gene": "atpF", "product": "F0F1 ATP synthase subunit B", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_Q9PR09", "cog_id": "COG0711", "cog_category": "C", "go_ids": [ "GO:0005886", "GO:0015078", "GO:0015986", "GO:0045263", "GO:0046933", "GO:0046961" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PR09", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR09" ], "product": "ATP synthase subunit b" }, "genes": [ "atpF" ], "id": "NKIECMJPJD_46", "locus": "GCA000828735_00005" }, { "type": "cds", "sequence": "contig_1", "start": 2413, "stop": 2952, "strand": "+", "frame": 1, "gene": "atpH", "product": "ATP synthase F1 subunit delta", "db_xrefs": [ "COG:C", "COG:COG0712", "EC:7.1.2.2", "GO:0005886", "GO:0015986", "GO:0045261", "GO:0046933", "RefSeq:WP_006688889.1", "SO:0001217", "UniParc:UPI00000C1BAD", "UniRef:UniRef100_Q9PR10", "UniRef:UniRef50_Q9PR10", "UniRef:UniRef90_Q9PR10" ], "nt": "ATGAAATCTTCATTAAAACCAGTTGAAAAATACGCTTATTCAATTTTTGAAATTGCTAAAGAAGAAAAAAAATTAGATTTATACAAACATAATTTGGAAACAATTAATTCTATCATTGAAGAGGTTCCAGCTTTTTTTGAAGCTGTTGGTGATCCTGCTCGTGATCGTAATGAAAGAAAACAAATTGTGATTAAAAATCTAGAAGGTGAGATTGATATTTATTTAATTAGTTTAATTGATCTACTAATTGATGTTAAATCGGTTAAACTACTTAAAAAAATAGTATTAAAAGCTTTGGATTTTGTTAATGAAGCTTTAAGTGTTAAAAAAGTTTTAATAACAACTGCTTATGAATTAACAAAAAATCAAATTGATCGCCTTCTGCAATCTTTAAAAAAGAAATATGTGTGTGAAAAAATAGAACCAATTGTTGTTGTTGATAAATCAATTATTGGAGGACTTTCAATTAATTTTGAATCGCAAGTTTTAGATAATAGTTTAAAAACTAAATTATTTAATATTGTAAAAAAGGTAAATTAG", "aa": "MKSSLKPVEKYAYSIFEIAKEEKKLDLYKHNLETINSIIEEVPAFFEAVGDPARDRNERKQIVIKNLEGEIDIYLISLIDLLIDVKSVKLLKKIVLKALDFVNEALSVKKVLITTAYELTKNQIDRLLQSLKKKYVCEKIEPIVVVDKSIIGGLSINFESQVLDNSLKTKLFNIVKKVN", "aa_hexdigest": "bd4b157a59989a77dc7267f934dc0c59", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00000C1BAD", "ncbi_nrp_id": "WP_006688889.1", "uniref100_id": "UniRef100_Q9PR10", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1BAD", "RefSeq:WP_006688889.1", "UniRef:UniRef100_Q9PR10" ] }, "ips": { "uniref100_id": "UniRef100_Q9PR10", "uniref90_id": "UniRef90_Q9PR10" }, "psc": { "uniref90_id": "UniRef90_Q9PR10", "gene": "atpH", "product": "ATP synthase F1 subunit delta", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_Q9PR10", "cog_id": "COG0712", "cog_category": "C", "go_ids": [ "GO:0005886", "GO:0015986", "GO:0045261", "GO:0046933" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PR10", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR10" ], "product": "ATP synthase subunit delta" }, "genes": [ "atpH" ], "id": "NKIECMJPJD_47", "locus": "GCA000828735_00006" }, { "type": "cds", "sequence": "contig_1", "start": 2962, "stop": 3261, "strand": "+", "frame": 1, "gene": "atpH", "product": "F-type ATPase subunit delta", "db_xrefs": [ "COG:C", "COG:COG0712", "RefSeq:WP_006688956.1", "SO:0001217", "UniParc:UPI00000C1BAC", "UniRef:UniRef100_Q9PR11", "UniRef:UniRef50_A0AAP9ABM6", "UniRef:UniRef90_A0AAP9ABM6" ], "nt": "ATGCAAAGTAATACTAAACTAGCAAAATATTCAAAAATTATCGCCAATCGTTTAAAAGCATATTCTAATCAAACTCGTTTTATTGAAATTAAAACTGCTTTTGAATTGAATGATGAACAAAAACAAAGAATTAAAAAAACTGTTATTAGTCGTTTTGGCGATGAAAGACCCATTAAATTTATCGTTGATCCATCATTAATTGGTGGTATATCATTAAAAATCAACTTGGAAATAATTGATTCTTCTCTAAAAACAAAACTAAATCAAATCATCAATACGAAAGAGAAAGAAGGTGCGTAA", "aa": "MQSNTKLAKYSKIIANRLKAYSNQTRFIEIKTAFELNDEQKQRIKKTVISRFGDERPIKFIVDPSLIGGISLKINLEIIDSSLKTKLNQIINTKEKEGA", "aa_hexdigest": "dd81625cb918389fae639d403c5e4b1f", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI00000C1BAC", "ncbi_nrp_id": "WP_006688956.1", "uniref100_id": "UniRef100_Q9PR11", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1BAC", "RefSeq:WP_006688956.1", "UniRef:UniRef100_Q9PR11" ] }, "ips": { "uniref100_id": "UniRef100_Q9PR11", "uniref90_id": "UniRef90_A0AAP9ABM6" }, "psc": { "uniref90_id": "UniRef90_A0AAP9ABM6", "gene": "AtpH", "product": "F-type ATPase subunit delta", "uniref50_id": "UniRef50_A0AAP9ABM6", "cog_id": "COG0712", "cog_category": "C" }, "pscc": { "uniref50_id": "UniRef50_A0AAP9ABM6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAP9ABM6" ], "product": "F-type ATPase subunit delta" }, "genes": [ "atpH" ], "id": "NKIECMJPJD_48", "locus": "GCA000828735_00007" }, { "type": "cds", "sequence": "contig_1", "start": 3264, "stop": 4013, "strand": "+", "frame": 3, "gene": null, "product": "ATP synthase subunit alpha", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A223M9E1" ], "nt": "ATGACAGATAATAAAAATCATTCATTAATTAGTGATATAAAATCACAAATTAAGAAATTTTCTGAAAAAGCTTTAACCTTAGAGGTCGGCAATGTAATTTCGTTAGGTGATGGAATTGTATTGGTTGATGGCCTTGATAATGTCATGTTAAATGAAATTGTACGTTTTGAAAATGGTGTTGAAGGAATGGCTTTAAACCTAGAAGAAGATGCTGTTGGTGTTGTTCTTTTAGGTGATTATTCAAATATTAAAGAAGGCGATCGTGTTTATCGTACAAAAAGAATTGTTGAAGTTCCTGTTGGGGATGTAATGTTAGGTCGTGTTGTTGACGCTTTAGGTAAGGCAGTTGATAATAAAGGTAATATTGTTGCAAATAAATTTAGTGTAATTGAAAAAATTGCACCTGGTGTTATGGACCGTAAATCTGTTCATCAACCACTTGAAACCGGAATTTTATCAATTGATGCAATGTTCCCAATTGGTAAGGGCCAAAGAGAATTAATTATTGGTGACCGACAGACTGGAAAAACAACAATTGCAATTGATGCTATTATTAATCAAAAAGGCCGTAATGTAAATTGTGTTTATGTAGCAATTGGTCAAAAAAACTCAACAATCGCAAATGTTGTTCGTGAACTTGAAGCTCATGGAGCAATGGAATATACAACTGTTGTTACAGCTAATGCTTCAGAATTACCTGCTTTGCAATATATTGCTCCCTTTACAGGAGTAACAATTGCTGAAGAGTGA", "aa": "MTDNKNHSLISDIKSQIKKFSEKALTLEVGNVISLGDGIVLVDGLDNVMLNEIVRFENGVEGMALNLEEDAVGVVLLGDYSNIKEGDRVYRTKRIVEVPVGDVMLGRVVDALGKAVDNKGNIVANKFSVIEKIAPGVMDRKSVHQPLETGILSIDAMFPIGKGQRELIIGDRQTGKTTIAIDAIINQKGRNVNCVYVAIGQKNSTIANVVRELEAHGAMEYTTVVTANASELPALQYIAPFTGVTIAEE", "aa_hexdigest": "caa9324e04d5abd7c491d7a0aa7de4f9", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "psc": { "uniref90_id": "UniRef90_A0A223M9E1", "query_cov": 0.9839357429718876, "subject_cov": 0.9607843137254902, "identity": 0.5429999999999999, "score": 258.0, "evalue": 2.3e-83, "valid": false, "product": "ATP synthase subunit alpha", "uniref50_id": "UniRef50_A0A223M9E1" }, "pscc": { "uniref50_id": "UniRef50_A0A223M9E1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A223M9E1" ], "product": "ATP synthase subunit alpha" }, "genes": [], "id": "NKIECMJPJD_49", "locus": "GCA000828735_00008" }, { "type": "cds", "sequence": "contig_1", "start": 4014, "stop": 5255, "strand": "+", "frame": 3, "gene": "atpA", "product": "F0F1 ATP synthase subunit alpha", "db_xrefs": [ "COG:COG4933", "COG:K", "EC:7.1.2.2", "GO:0005524", "GO:0005886", "GO:0015986", "GO:0043531", "GO:0045261", "GO:0046933", "GO:0046961", "SO:0001217", "UniRef:UniRef50_Q9PR12", "UniRef:UniRef90_Q9PR12" ], "nt": "ATGCATCAAGGAAAAGATGTTTTAATTGTTTATGATGATCTAAGCAAACATGCTATTGCTTATCGTACTTTATCCTTATTATTACGTCGTCCGCCTGGTCGTGAAGCATATCCTGGAGATGTTTTTTACTTACACAGTCGTTTATTAGAACGTGCATGCAAACTAAAAGATGAATTAGGGGCAGGATCTATTACTGCTTTACCAATTATTGAAACACAAGCTGGTGATATTTCAGCTTATATTCCAACAAATGTAATTTCAATTACTGATGGGCAAATTTTTATGATGACATCATTATTTAATGCTGGTCAACGTCCTGCAATTGATGCAGGGCAATCAGTTAGTCGGGTAGGTTCTGCTGCACAAATTAAATCAGTTAAACAAACTGGAGCATCATTAAAATTAGAATTAGCTAACTATCGCGAACTTGAAGCATTTAGTCAATTCGGTAGTGATTTGGATGATGAAACTAAACGTATTCTAAAACTTGGTAAAGCAGTTATGGCTGTTATTAAGCAAGCTCCTAATAAACCTTATAATCAAACTGATGAAGCGATCATTTTGTTTACCGTTAAAGAAAAACTTATTCCTCAAGTTCCAGTAGAACGTATTCAAGATTTTAAAGAGTACTTATTAAATTACTTTAAAGGAACAAAACTACGTGCTGATCTTGAAGATAAGAAAGCTTTTGATAAAGAAAATACCCCAGCATTTAGATGTGCTATACAAAAAGCAATTAACAGTTTCTTAAATAATTCACAAGATTTTAAACCTTGTGAAGAAGCTGAACAAACAGCCTTTGATAAATTCTTTAATGAGAATGAATCAATTGTTGTTGATGGTGAAAATGATTTTAATTTTATTAATGAAGAAGTTAGTTTAAAACCTACAACAGAAACATCTGAAGCTGTTCAAATAGAAGAAAAAGTTCAAGATTTCGTTGAACCACAAGAAATTCTTGAAACTAATAAAATGGAAGAAAATCATATTTTTGAAGAAGTTGAGCCAGAAAAAATTATCTGTGAACATCATGAATTTGAAATAGCTGAAAACCAAGAAAAAATCGAAGGTCAACAAGTTTTAGAAGATACAAACCATGAATATTCAATTTATGAAACCGTTGAACAAAGCGGTGAGGTAGATAATGATGAATCTAAAGATGATGATTTAGAAGTCTTAGTACCAGTTGCTGAAATTGAGCATGATGAAGCAATTCTAGATGAACGCGAGAATCGTAATTGA", "aa": "MHQGKDVLIVYDDLSKHAIAYRTLSLLLRRPPGREAYPGDVFYLHSRLLERACKLKDELGAGSITALPIIETQAGDISAYIPTNVISITDGQIFMMTSLFNAGQRPAIDAGQSVSRVGSAAQIKSVKQTGASLKLELANYRELEAFSQFGSDLDDETKRILKLGKAVMAVIKQAPNKPYNQTDEAIILFTVKEKLIPQVPVERIQDFKEYLLNYFKGTKLRADLEDKKAFDKENTPAFRCAIQKAINSFLNNSQDFKPCEEAEQTAFDKFFNENESIVVDGENDFNFINEEVSLKPTTETSEAVQIEEKVQDFVEPQEILETNKMEENHIFEEVEPEKIICEHHEFEIAENQEKIEGQQVLEDTNHEYSIYETVEQSGEVDNDESKDDDLEVLVPVAEIEHDEAILDERENRN", "aa_hexdigest": "803431589ce2d4b4de81bc5275ff8173", "start_type": "ATG", "rbs_motif": null, "genes": [ "atpA" ], "truncated": "both", "pseudogene": { "start": 3714, "stop": 5558, "observations": { "mutation": [ 4011, 5253, 5484 ], "stop": [ 4011, 5253, 5484 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PR12", "query_cov": 1.0, "subject_cov": 0.5168961201501877, "identity": 1.0, "score": 783.0, "evalue": 1.85e-279, "gene_start": 1, "gene_end": 413, "reference_start": 251, "reference_end": 663, "reference_sequence": "MTDNKNHSLISDIKSQIKKFSEKALTLEVGNVISLGDGIVLVDGLDNVMLNEIVRFENGVEGMALNLEEDAVGVVLLGDYSNIKEGDRVYRTKRIVEVPVGDVMLGRVVDALGKAVDNKGNIVANKFSVIEKIAPGVMDRKSVHQPLETGILSIDAMFPIGKGQRELIIGDRQTGKTTIAIDAIINQKGRNVNCVYVAIGQKNSTIANVVRELEAHGAMEYTTVVTANASELPALQYIAPFTGVTIAEEWMHQGKDVLIVYDDLSKHAIAYRTLSLLLRRPPGREAYPGDVFYLHSRLLERACKLKDELGAGSITALPIIETQAGDISAYIPTNVISITDGQIFMMTSLFNAGQRPAIDAGQSVSRVGSAAQIKSVKQTGASLKLELANYRELEAFSQFGSDLDDETKRILKLGKAVMAVIKQAPNKPYNQTDEAIILFTVKEKLIPQVPVERIQDFKEYLLNYFKGTKLRADLEDKKAFDKENTPAFRCAIQKAINSFLNNSQDFKPCEEAEQTAFDKFFNENESIVVDGENDFNFINEEVSLKPTTETSEAVQIEEKVQDFVEPQEILETNKMEENHIFEEVEPEKIICEHHEFEIAENQEKIEGQQVLEDTNHEYSIYETVEQSGEVDNDESKDDDLEVLVPVAEIEHDEAILDERENRNWVFSDSAVSEVEKQTIMISISSNEAEQLFDNAKSVVFFKVTPKYPVEKVLVYVTSPVQKVVGEFDLLKIDVNSVNTSWNKYRSSSVISSRKEYLEYFNSHKEAHALLASKVYKYRKPKDLASFNMNKGPSGFTYLK" }, "paralog": false, "identity": 0.995114006514658, "score": 1187.0, "evalue": 0.0, "description": "Internal stop codon at 4011, 5253, 5484. Nonsense mutation around 4011, 5253, 5484.", "psc": { "uniref90_id": "UniRef90_Q9PR12", "gene": "atpA", "product": "F0F1 ATP synthase subunit alpha", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_Q9PR12", "cog_id": "COG4933", "cog_category": "K", "go_ids": [ "GO:0005524", "GO:0005886", "GO:0015986", "GO:0043531", "GO:0045261", "GO:0046933", "GO:0046961" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PR12", "EC:7.1.2.2", "UniRef:UniRef50_Q9PR12", "COG:COG4933", "COG:K", "GO:0005524", "GO:0005886", "GO:0015986", "GO:0043531", "GO:0045261", "GO:0046933", "GO:0046961" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PR12", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR12" ], "product": "ATP synthase subunit alpha" } }, "id": "NKIECMJPJD_50", "locus": "GCA000828735_00009" }, { "type": "cds", "sequence": "contig_1", "start": 5301, "stop": 5486, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTTCAATTTCATCAAATGAAGCAGAACAATTGTTTGATAATGCTAAAAGTGTTGTTTTCTTTAAAGTAACACCTAAATATCCAGTTGAAAAAGTATTAGTTTATGTCACAAGTCCAGTACAAAAAGTTGTTGGTGAATTTGATTTATTAAAGATTGATGTTAATTCAGTAAACACTTCTTGA", "aa": "MISISSNEAEQLFDNAKSVVFFKVTPKYPVEKVLVYVTSPVQKVVGEFDLLKIDVNSVNTS", "aa_hexdigest": "413a38f8911b41dfdc59b80217a5023c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6792.754400000003, "isoelectric_point": 5.096036338806152 }, "id": "NKIECMJPJD_51", "locus": "GCA000828735_00010" }, { "type": "cds", "sequence": "contig_1", "start": 5792, "stop": 5908, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTGGTTTATTAAATGAAGGTTTTGAAAATGCAAGAGCATTAGTTAATGATCAAGGTCAAATTATTGGTAAAGTAGCTAATGTTAGTGGTGATAATCATTCTTTAATGTCATGA", "aa": "MVGLLNEGFENARALVNDQGQIIGKVANVSGDNHSLMS", "aa_hexdigest": "eff3116122d40d6137dc65d0b88543d1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3999.4443, "isoelectric_point": 4.749261665344237 }, "id": "NKIECMJPJD_52", "locus": "GCA000828735_00011" }, { "type": "cds", "sequence": "contig_1", "start": 5942, "stop": 6088, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTATAAAACAATTCACGATCAATTGATTAATGGAAAAACAATGGAGTCATTAATTAATAATACTAATAAAAATAACGATCCAAAATTATATGAAGCTCTTCATTATCATGCGCATAGTAAATTTATTAATCATAATGGAACATGA", "aa": "MYKTIHDQLINGKTMESLINNTNKNNDPKLYEALHYHAHSKFINHNGT", "aa_hexdigest": "efea3345a492ee04653196a8da2a097a", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5610.216100000001, "isoelectric_point": 8.126384925842284 }, "id": "NKIECMJPJD_53", "locus": "GCA000828735_00012" }, { "type": "cds", "sequence": "contig_1", "start": 6284, "stop": 6469, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATGCCACAAATGATTACTTTTATAATTATTGTTGTTAAACTAGCGACTGTATTTTCACCTAAAAAAAGTGCTGAAGAAAAACAAGCTTATAAACTTGCAAAACTTGAAGTTAAAAATCAAAAAAAGGCTAAAAAATTAAATAATTCTTTACAACAAATAAATTTAAATTTAGAGGCAAACTAG", "aa": "MMPQMITFIIIVVKLATVFSPKKSAEEKQAYKLAKLEVKNQKKAKKLNNSLQQINLNLEAN", "aa_hexdigest": "98b828acec040d16033afa690ad23f43", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6959.289700000001, "isoelectric_point": 10.000158500671386 }, "id": "NKIECMJPJD_54", "locus": "GCA000828735_00013" }, { "type": "cds", "sequence": "contig_1", "start": 6471, "stop": 7331, "strand": "+", "frame": 3, "gene": "atpG", "product": "ATP synthase F1 subunit gamma", "db_xrefs": [ "COG:C", "COG:COG0224", "EC:7.1.2.2", "GO:0005524", "GO:0005886", "GO:0015986", "GO:0042777", "GO:0045261", "GO:0046933", "RefSeq:WP_006688922.1", "SO:0001217", "UniParc:UPI00000C1BAA", "UniRef:UniRef100_Q9PR14", "UniRef:UniRef50_Q9PR14", "UniRef:UniRef90_Q9PR14" ], "nt": "ATGTCTTTAGATGCAATGAAACGAAAAATTAACTCAGTTCAAACAACTGCTAAGATTACGAATGCAATGAAACTAGTGGCAACTGCAAAATTAAAACGTCAGCGTGATCGTTTAGCTGCAATTAAAGAATATTGTCATGATTACTATGATGTTATTGGGTTATTATTGTCTGTTGTTGATGATGTTGAATTTTTAAAAATTCCAAATGCGAAAGATCGAACTTTATATATCACTATTAATTCAACAATGGGTTTAGCGGGTTCTTATAACTATAATGTTAATAAATTAATTTCTAAAATTGTAAATGAAACTGATATAACATTTACAATTGGAAAAAAAGGACATGACTTTATGCGTTTATCAGGTCGTGTTGATCAAGTTAATACTTATTTGAATTTAAATGATAATGATTTAACATTTGACATGAGTTTACAAATTGCGCGCGAGGCTCTTGAATTATATTCACAAGGTGAAGTTAATAAAATTTGTATTATATATACTAAATTTATTAACGCAATTACTTTTGAAGTAAGTGTTATAGATGTTCTTCCATTCGATAAGACAGCTTTAATTAAAGATCATCTTGCTGAAACTATTGAATTAGCAAAAGATAATATTATTTTTAAACCTAACAAGTTTGAGCTTGTTAAAAAAATTCTACCTACTTATATTGCAACAGTTCTATATGGTTCATTAATTGAATCAAAAATCTCTGAAAATGCATCACGACGCAATGCAATGGATGCAGCTACAAAAAATGCCAAAGCATTGGCTGAAAATTATAAATTAATTTACAATACATTACGCCAAGGTAAAATTACTCGTGAAATTACTGAAATTGTTGCTGGTAGTGATGATTAG", "aa": "MSLDAMKRKINSVQTTAKITNAMKLVATAKLKRQRDRLAAIKEYCHDYYDVIGLLLSVVDDVEFLKIPNAKDRTLYITINSTMGLAGSYNYNVNKLISKIVNETDITFTIGKKGHDFMRLSGRVDQVNTYLNLNDNDLTFDMSLQIAREALELYSQGEVNKICIIYTKFINAITFEVSVIDVLPFDKTALIKDHLAETIELAKDNIIFKPNKFELVKKILPTYIATVLYGSLIESKISENASRRNAMDAATKNAKALAENYKLIYNTLRQGKITREITEIVAGSDD", "aa_hexdigest": "8c50bb71c02373d5a07ca338e4bd0993", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI00000C1BAA", "ncbi_nrp_id": "WP_006688922.1", "uniref100_id": "UniRef100_Q9PR14", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1BAA", "RefSeq:WP_006688922.1", "UniRef:UniRef100_Q9PR14" ] }, "ips": { "uniref100_id": "UniRef100_Q9PR14", "uniref90_id": "UniRef90_Q9PR14" }, "psc": { "uniref90_id": "UniRef90_Q9PR14", "gene": "atpG", "product": "ATP synthase F1 subunit gamma", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_Q9PR14", "cog_id": "COG0224", "cog_category": "C", "go_ids": [ "GO:0005524", "GO:0005886", "GO:0015986", "GO:0042777", "GO:0045261", "GO:0046933" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PR14", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR14" ], "product": "ATP synthase gamma chain" }, "genes": [ "atpG" ], "id": "NKIECMJPJD_55", "locus": "GCA000828735_00014" }, { "type": "cds", "sequence": "contig_1", "start": 7433, "stop": 8752, "strand": "+", "frame": 2, "gene": "atpD", "product": "F0F1 ATP synthase subunit beta", "db_xrefs": [ "COG:C", "COG:COG0055", "EC:7.1.2.2", "GO:0005524", "GO:0005886", "GO:0015986", "GO:0016887", "GO:0045261", "GO:0046933", "GO:0046961", "SO:0001217", "UniRef:UniRef50_P0A300", "UniRef:UniRef90_Q9PR15" ], "nt": "TTGCCTGAAATTAACACTGCTTTAGAATTAAACAATCATGGTTCTAAATTAGTACTTGAGGTTTCTCAATTAGTTGGTGATAATATTGCAAGATGTATTGCAATGGATACAACTGATGGACTAGTACGTGGACAAGAGGTAATTAATACTCAAAAACCAATCACAATGCCTGTTGGTAAACAAGTACTTGGACGTATGTTCAATGTAACTGGTGATCCAATTGATGAACAACCTGCACCAACAGGTAAACGTATGCCAATTCATCGTCCTGCACCTTCATTCGCCGAACAAGCAGAATCAATTGAAATCCTAGAAACGGGAATTAAAGTTGTCGACTTACTTGTACCTTTTGCAAAGGGTGGTAAGATCGGTTTATTTGGTGGTGCTGGAGTTGGTAAAACAGTTTTAATGCAAGAATTAATTCATAACATCGCTAAAAATCATGGTGGTTTATCAGTTTTTGCTGGAGTTGGAGAAAGAACACGTGAAGGAAATGATTTATACTATGAAATGGCTGAGTCAGATGTTTTAGATAAAACAGCTCTTGTCTTTGGACAAATGAATGAACCACCAGGAGCACGTATGCGCGTCGCATTAAGCGGTTTAACAATGGCTGAAGAATTCCGTGATGCTTTTGGTCAAGATGTTTTATTATTTATCGATAACATTTTTAGATTCACACAAGCTGGTTCAGAAGTTTCTGCTTTACTTGGACGAATGCCATCAGCAGTTGGTTATCAACCAACACTTGCTTTTGAAATGGGTCAATTACAAGAACGAATTACATCAACTAAAAAAGGATCAATTACATCGGTTCAAGCTGTTTATGTTCCAGCTGATGACTTAACTGACCCCGCACCAGCAACAACTTTTTCACACTTAGATGCTAAAGTTGTTTTAGATAGAGCTATTGCCTCATTGGGATTATATCCAGCAATTTCACCACTACAATCAACAAGTCGTTTATTAGATCCATTAGTTGTTGGTGTAAAACACTACAGTGTTGCGCGTCGTGTAATTGAAATTTTACAAAGATTTATGGAATTACAAGATATTATTGCAATTCTTGGAATGGATGAATTAAGCGAAGAAGATCGTCAATTAGTAATGCGCGCACGTAAAGTACGTAACTACTTATCACAGCCAAGCCACGTTGCTGAAAAATTCTCTGGTCAACCTGGATTATCAGTTAAACTTGAAGATACTATTGAAGGATTTAGAAAAATTCTTGATGGTGAATGTGATGATATTCATGAACAACATTTCTTATATGTTGGTAAAATTGATGACGTTTTTGAAAAAGCAGCAAAAAATAAATAA", "aa": "MPEINTALELNNHGSKLVLEVSQLVGDNIARCIAMDTTDGLVRGQEVINTQKPITMPVGKQVLGRMFNVTGDPIDEQPAPTGKRMPIHRPAPSFAEQAESIEILETGIKVVDLLVPFAKGGKIGLFGGAGVGKTVLMQELIHNIAKNHGGLSVFAGVGERTREGNDLYYEMAESDVLDKTALVFGQMNEPPGARMRVALSGLTMAEEFRDAFGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQPTLAFEMGQLQERITSTKKGSITSVQAVYVPADDLTDPAPATTFSHLDAKVVLDRAIASLGLYPAISPLQSTSRLLDPLVVGVKHYSVARRVIEILQRFMELQDIIAILGMDELSEEDRQLVMRARKVRNYLSQPSHVAEKFSGQPGLSVKLEDTIEGFRKILDGECDDIHEQHFLYVGKIDDVFEKAAKNK", "aa_hexdigest": "390d37d1c6d7e8f32c2b105c9ac28b73", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_Q9PR15", "query_cov": 1.0, "subject_cov": 0.9461206896551724, "identity": 0.998, "score": 852.0, "evalue": 1.13e-311, "valid": true, "gene": "atpD", "product": "F0F1 ATP synthase subunit beta", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_P0A300", "cog_id": "COG0055", "cog_category": "C", "go_ids": [ "GO:0005524", "GO:0005886", "GO:0015986", "GO:0016887", "GO:0045261", "GO:0046933", "GO:0046961" ] }, "pscc": { "uniref50_id": "UniRef50_P0A300", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P0A300" ], "product": "ATP synthase subunit beta" }, "genes": [ "atpD" ], "id": "NKIECMJPJD_56", "locus": "GCA000828735_00015" }, { "type": "cds", "sequence": "contig_1", "start": 8752, "stop": 9162, "strand": "+", "frame": 1, "gene": "atpC", "product": "ATP synthase F1 subunit epsilon", "db_xrefs": [ "COG:C", "COG:COG0355", "EC:7.1.2.2", "GO:0005524", "GO:0005886", "GO:0015986", "GO:0045261", "GO:0046933", "RefSeq:WP_006688897.1", "SO:0001217", "UniParc:UPI0000126524", "UniRef:UniRef100_Q9PR16", "UniRef:UniRef50_Q9PR16", "UniRef:UniRef90_Q9PR16" ], "nt": "ATGGCTAATTTAACTAAATTAAAAATTGTTACACCTTATGCACAAATTTTAGAAAAAGATGTGTATAGTGTTGAATTAAAAACATCAGAAGGACGAATTACCGTTTTACCTGACCATAACCCTTTGATGTCAACAATTGAAAACCATGTTGCTTATATTCGTGAATTACCAAATACTCCACGTAAACCACTATTATTATTAGATGGTATAGTTTATATTGAACAACATCAAGTTCGTGTTTTTAGTGACTATTTTAAATTTTTAGATGAAATTCAAATTGATGAAATTAATTCTTCGTTAAATCAATTAAAACATGATTTAAATAATGAAGAAGATGATAAGAAAAAACTTCAATTAAAATCTAAAATTAAATTAAACGAATCAATTTTAATCGCTTATAAAGATCGATAA", "aa": "MANLTKLKIVTPYAQILEKDVYSVELKTSEGRITVLPDHNPLMSTIENHVAYIRELPNTPRKPLLLLDGIVYIEQHQVRVFSDYFKFLDEIQIDEINSSLNQLKHDLNNEEDDKKKLQLKSKIKLNESILIAYKDR", "aa_hexdigest": "5dc40439f496b8b7880540b377fbe853", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "ups": { "uniparc_id": "UPI0000126524", "ncbi_nrp_id": "WP_006688897.1", "uniref100_id": "UniRef100_Q9PR16", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000126524", "RefSeq:WP_006688897.1", "UniRef:UniRef100_Q9PR16" ] }, "ips": { "uniref100_id": "UniRef100_Q9PR16", "uniref90_id": "UniRef90_Q9PR16" }, "psc": { "uniref90_id": "UniRef90_Q9PR16", "gene": "atpC", "product": "ATP synthase F1 subunit epsilon", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_Q9PR16", "cog_id": "COG0355", "cog_category": "C", "go_ids": [ "GO:0005524", "GO:0005886", "GO:0015986", "GO:0045261", "GO:0046933" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PR16", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR16" ], "product": "ATP synthase epsilon chain" }, "genes": [ "atpC" ], "id": "NKIECMJPJD_57", "locus": "GCA000828735_00016" }, { "type": "cds", "sequence": "contig_1", "start": 9306, "stop": 9611, "strand": "+", "frame": 3, "gene": null, "product": "putative protein UU127", "db_xrefs": [ "GO:0005886", "RefSeq:WP_006688845.1", "SO:0001217", "UniParc:UPI0000139305", "UniRef:UniRef100_Q9PR17", "UniRef:UniRef50_Q9PR17", "UniRef:UniRef90_Q9PR17" ], "nt": "ATGAAGATTATTGGGTCAGCTTTTTTAGGAATAGTTTTTTGCATTCTTTTGGCTTTTGCAATTATTTTTGGAATCGAAATTGATTATTATCATCAAGGTGATTATTTGAAATATCTTAATTTTTTAGATAAATTACACCAATATAATAAAATTGATAATTCATTTGAATACTCAAATCATTATGAATCAGCATTAATTGGTGTAATTGTTTTAACAATTATTTGCTTTTTAATATTCATAACACCAATTATAATAATAGTAATAACAAAGATTAAAGAAAAGAAAGTAATTAACAAAAAGATTTAA", "aa": "MKIIGSAFLGIVFCILLAFAIIFGIEIDYYHQGDYLKYLNFLDKLHQYNKIDNSFEYSNHYESALIGVIVLTIICFLIFITPIIIIVITKIKEKKVINKKI", "aa_hexdigest": "5235241beb0539541d1f19c11bfe5153", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0000139305", "ncbi_nrp_id": "WP_006688845.1", "uniref100_id": "UniRef100_Q9PR17", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139305", "RefSeq:WP_006688845.1", "UniRef:UniRef100_Q9PR17" ] }, "ips": { "uniref100_id": "UniRef100_Q9PR17", "uniref90_id": "UniRef90_Q9PR17" }, "psc": { "uniref90_id": "UniRef90_Q9PR17", "product": "Uncharacterized protein UU127", "uniref50_id": "UniRef50_Q9PR17", "go_ids": [ "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PR17", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR17" ], "product": "Uncharacterized protein UU127" }, "genes": [], "id": "NKIECMJPJD_58", "locus": "GCA000828735_00017" }, { "type": "cds", "sequence": "contig_1", "start": 9810, "stop": 10583, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAAAAAAAATATTAAAAAACTTATCAATAGGTGTTGTATCAACAATAATTCTTGGTTCAATAACTATATCATTATCTAATTGTTCTTATAACAAAAAAACATTTTCGTCTTCTACAATTACGAATCAAGATACTAAAAATTTAGACATAATAGCAAAAAACATTAAATTTAAATATGATATTTTTGAAAATGAAGATGAGCAAATAATTAAAATTAATAAAATTAAAGTTGTGGGTGACTTGGGGCAAGCTAAAATAATAATTAAAAAAGTTATTATTGAACAAAAGTCAAATAATTTTTTTGTTCAATACCAACTCAGTTTGCATAACCAAACTAAAGAATATACACAAAACTTAGAAATCAATAAGAAGCAAAGTGTTATTGAAACAAAATATATTAATGATGAATTAATAAATAATGATTATCGTCCTTTTGCATTAAAACAATTATTATCTTCACCGCAAAACATAAAAAAAACTAGTGATCTTTCTTTAATAACAATGCAGAAAAATAACCAAGAATTTAATAATTATTTTAATTTTATTCAAAATGAAGATAATTTTAAACAACTACCCTTTAATCAAGCAACGCTCTTAACAAAACAAAACCAACTAACAGATCATCACACTAACTTTAAATTTGAAAAAATTGAAATAGATAAGCAAAACCAAATTGCAACAGTTAGTTTTAGTCATGATTCTGATTCACAAACAATACAAAAAATATTAAATGCGACAAATAAACGTCTTTTGATTAAAAATTTTGATTGA", "aa": "MQKKILKNLSIGVVSTIILGSITISLSNCSYNKKTFSSSTITNQDTKNLDIIAKNIKFKYDIFENEDEQIIKINKIKVVGDLGQAKIIIKKVIIEQKSNNFFVQYQLSLHNQTKEYTQNLEINKKQSVIETKYINDELINNDYRPFALKQLLSSPQNIKKTSDLSLITMQKNNQEFNNYFNFIQNEDNFKQLPFNQATLLTKQNQLTDHHTNFKFEKIEIDKQNQIATVSFSHDSDSQTIQKILNATNKRLLIKNFD", "aa_hexdigest": "81247da86bcc501a700306d7a7658e4c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 29864.706000000027, "isoelectric_point": 9.288363075256349 }, "id": "NKIECMJPJD_59", "locus": "GCA000828735_00018" }, { "type": "cds", "sequence": "contig_1", "start": 10797, "stop": 10991, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAAGAAAATGAAGTATATGGATCAGCATTTTTTGATTTTAATGAACAAACTTTAAAATTATATAAGAACAAGATTTTTTTATTTGAGTTTACTCTAGACGAAAATTATAGCGAAGCTAAAAAAAATCAAAGTTTAATTGCAAAATATACTCCACCAATTCAGTATGTTCGTGTTCCTTTTAAATCGCTATGA", "aa": "MQENEVYGSAFFDFNEQTLKLYKNKIFLFEFTLDENYSEAKKNQSLIAKYTPPIQYVRVPFKSL", "aa_hexdigest": "3a26315c376143a7079ebb0d91febe03", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7665.6372, "isoelectric_point": 6.168247032165527 }, "id": "NKIECMJPJD_60", "locus": "GCA000828735_00019" }, { "type": "cds", "sequence": "contig_1", "start": 11286, "stop": 11603, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCGAAATTTTATTTCATTAGTTCAATATGAAAATACCTACTTATTTTATCAAAATAAAACAAACGAAGGTTTTTATGATTTCATAACTAAAAAAGCTGATTTTATACTTGTAAATAATAATGAAATCAAAAATTTACATAAAACTACATTAAAAGATAATCCAGATTTGATCTTTAAAAAAGATAAAGATAATTATTTTCAATTAACAAAAAAAATAAATAAGATTAAAAATTTAAATCTTGTAAATGAAAAAAATTTAGTTTTTAATATTACTTTTGGTTTAGATCCAAATCTCATCAAAGAAACTAACCTGTGA", "aa": "MRNFISLVQYENTYLFYQNKTNEGFYDFITKKADFILVNNNEIKNLHKTTLKDNPDLIFKKDKDNYFQLTKKINKIKNLNLVNEKNLVFNITFGLDPNLIKETNL", "aa_hexdigest": "7ec4f7f6e31ae5363fc84e751af4164b", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12556.27779999998, "isoelectric_point": 9.24742565155029 }, "id": "NKIECMJPJD_61", "locus": "GCA000828735_00020" }, { "type": "cds", "sequence": "contig_1", "start": 11613, "stop": 11843, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAAGGTCTCAAATTTCATTAGCAGTCCCTTATAAAACTTTAAAAAATAAAAAAGTTATTGAAGATGCTGAATTTGTTTTAAATTATGGTATTGGAAATCAAGAGTACGAATATGAACTCTACAAAAAAATTAAGCAAGATTTTCGTTTTCAAATATCTTATATAAATGATGAATTAAAAATTAAAATTATTTCTAAAAATAACATTAGTGTTTTTGATAATATTTGA", "aa": "MLRSQISLAVPYKTLKNKKVIEDAEFVLNYGIGNQEYEYELYKKIKQDFRFQISYINDELKIKIISKNNISVFDNI", "aa_hexdigest": "b8a7ee8f46a3e8287ab1668afe48f925", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9067.375899999992, "isoelectric_point": 8.830121803283692 }, "id": "NKIECMJPJD_62", "locus": "GCA000828735_00021" }, { "type": "cds", "sequence": "contig_1", "start": 11931, "stop": 12059, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAGATGATAAATCTAATAATCAATATACAACAAGAATTTATAAAAACAATGAACAGAATTTTTTACCAATTAAAAATGCTCGTGATCGTGTAATTTCATTAAGTTTAATTCAAGATGGTACATGA", "aa": "MEDDKSNNQYTTRIYKNNEQNFLPIKNARDRVISLSLIQDGT", "aa_hexdigest": "dbc250d66352f5e2a666a5e574fb86e2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4944.4085000000005, "isoelectric_point": 6.026888465881346 }, "id": "NKIECMJPJD_63", "locus": "GCA000828735_00022" }, { "type": "cds", "sequence": "contig_1", "start": 12654, "stop": 13064, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTCAACTTTCCCTAGAACAGAGACAATTAATGAATTTAATGCTGGCAAACTTCCACGCTATCGTGAATATGAAATTAATAAATATGATAAAATCCAAGAAAATATTATTACTACTAATAATTACTTATCAACAGTAGGGACAACTAAAATTAGTAATCAATATTTTGATGTTGGTTCTGGTTCATCAGGATCAGTAGTTTATGATGAAAATGGTGATTTAGTTGGAATGATTTCTTTTGGCGCCCATGAAAATGCAAAATTTACAGCCGGAGGTATGATTGTTTTTGATTCTCTAATTTCTGGATTTTTTGGAGATAAAAACAATTTCGCTAATCGTACATCTTTAATTCAACAAATCAAACAATTAGCCTATTTATATCCAGAAAAATATGAAGATATTTATAAATAG", "aa": "MSTFPRTETINEFNAGKLPRYREYEINKYDKIQENIITTNNYLSTVGTTKISNQYFDVGSGSSGSVVYDENGDLVGMISFGAHENAKFTAGGMIVFDSLISGFFGDKNNFANRTSLIQQIKQLAYLYPEKYEDIYK", "aa_hexdigest": "94f990b6094b86d57dde40f0f5d4328f", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15376.945599999992, "isoelectric_point": 5.125763130187988 }, "id": "NKIECMJPJD_64", "locus": "GCA000828735_00023" }, { "type": "cds", "sequence": "contig_1", "start": 13076, "stop": 13969, "strand": "+", "frame": 2, "gene": null, "product": "Lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAP9AC84", "UniRef:UniRef90_A0AAC9X6P0" ], "nt": "ATGAAAAAAAATAAATATAAACTTCTTATTAAATCACTAGTTATTACATCTTTTTTAGTTACTACTTCAAGTGTTATTGTAGCTTGTACTAATAAAGAAAAAGAGCAACATTTAACTGAATTAAAAAAATTATCTCAACATGCAAAACAAATATATAATGAAATTAAAACTAATGAATTATTTAAAAAGGAGGCTTCTGTTTTAAATGATCAAATTAATAAAGTTTTAAAAATTAATGAACAAAATAATTTAGATGTCATTACTAAAAATTATAATGATTTAAAAAAATTATTAGATGAAATTCAAGAGAAAATTTCTAAAGAAAAAGAAAAAGAAAAGCAACAAAACGCTTATCTTAATAAAGAAATTAACATTAAATTAGAAAATTTAAATCAAATAGTTAATCATAATTTATTAAAAGGAAATAAAGAATCATTTTTTAATCAAATTATTAATTTCACTCCAATTACAACAGAAATTCAAACTATTATCAATCAAAATCAAAATCTTTTAGATTTCAAAAATGAAGAACTTGAATTAAAATTAAAAAATTTAGACCTTTTCATTAAAGAATTAGATCATAAATTTAAAAATATAATAGATCAACTATTTAATATTGAAGATTCATACATTAATAAGTTTCTAAAATTTATTAATAACCAGGACTTTATTAAGGATCAAAAATATCAAGAAAGAGAAAAGTTTAATCCGCAAGAATATTTAGAATTAACAAAATCTATTCAAAGTAGTTTTAATAAATTAAAAAACGTTACTATCAATTCAAACCTAGACCAATACCTAAATTCACTTAATTTAGTGTTATTATCTTTAACTAATGAAACTAAAGCCTTTTTTACTAAATTTGAAGATCAAATTAAAAAATATTTTAAGTGA", "aa": "MKKNKYKLLIKSLVITSFLVTTSSVIVACTNKEKEQHLTELKKLSQHAKQIYNEIKTNELFKKEASVLNDQINKVLKINEQNNLDVITKNYNDLKKLLDEIQEKISKEKEKEKQQNAYLNKEINIKLENLNQIVNHNLLKGNKESFFNQIINFTPITTEIQTIINQNQNLLDFKNEELELKLKNLDLFIKELDHKFKNIIDQLFNIEDSYINKFLKFINNQDFIKDQKYQEREKFNPQEYLELTKSIQSSFNKLKNVTINSNLDQYLNSLNLVLLSLTNETKAFFTKFEDQIKKYFK", "aa_hexdigest": "5008dff98922e96c5757ef7e92a5a537", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 13076, "stop": 14260, "observations": { "mutation": [ 13967, 14165, 14198 ], "stop": [ 13967, 14165, 14198 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAC9X6P0", "query_cov": 1.0067340067340067, "subject_cov": 0.7550505050505051, "identity": 0.9570000000000001, "score": 452.0, "evalue": 5e-157, "gene_start": 1, "gene_end": 297, "reference_start": 1, "reference_end": 299, "reference_sequence": "MKKNKYKLLIKSLVITSFLVTTSSVIVACTNKEKEQHLTKLKKLSQHAKQIYNEIKTNELFKNEASVLNDQINKVLKINEQNNLDVITKNYNDLKKLLDEIQEKISKEKEKEKQQNAYLNKEINIKLENLNQIVNHNLLKENKESFFNQIINFTPITTEIQTIINQNQNQNLLDFKNEELELKLKNLDLFIKELDHKFKNIIDQLFNIEDSYINKFLKFINNQDFIKDQKYKEREKFNPQEYLELTKSIESSFNKLKNVTINSNLDQYLNSLNLVLLSLTNETKTFFTKFEDQIKKYFKWIAYGKDDTLTKTFKRIVQEQKRNEFIEQTEKALLTFPTEINQKFKMFVDFYTLKNDKDETEYQKIWKSKRNEVDKVWFGIAECFGSGKCTDKSKPS" }, "paralog": false, "identity": 0.9267676767676768, "score": 643.0, "evalue": 7.58e-235, "description": "Internal stop codon at 13967, 14165, 14198. Nonsense mutation around 13967, 14165, 14198.", "psc": { "uniref90_id": "UniRef90_A0AAC9X6P0", "product": "Lipoprotein", "uniref50_id": "UniRef50_A0AAP9AC84", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAC9X6P0", "UniRef:UniRef50_A0AAP9AC84" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAP9AC84", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAP9AC84" ], "product": "Lipoprotein" } }, "id": "NKIECMJPJD_65", "locus": "GCA000828735_00024" }, { "type": "cds", "sequence": "contig_1", "start": 14269, "stop": 14400, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAGTCCATTAATTTTAAAAAATGCAGATCAAAGCATACGAATTGATATGTTTGGTGATGTACAATCTTTAAATGTTGGTGTAGCAACAGGAATTATCTTAGCGCATTATCGCACTAAAATAAAACCCTAG", "aa": "MSPLILKNADQSIRIDMFGDVQSLNVGVATGIILAHYRTKIKP", "aa_hexdigest": "322cdd67d3bb400dd1f7324630959348", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4725.5344000000005, "isoelectric_point": 9.520965003967284 }, "id": "NKIECMJPJD_66", "locus": "GCA000828735_00025" }, { "type": "cds", "sequence": "contig_1", "start": 14494, "stop": 14643, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGATGCTATTATTTATAAAAAGCATCACCAAGCAAGCATTAATGATTTAGTAATTAAAACAAGTATGGGTGCAATTAATTATATTTATATGATCGAAGTCAATAATTTATCAAATACAATAAAAAACCTTCAAGATGAAGGTTTTTGA", "aa": "MDAIIYKKHHQASINDLVIKTSMGAINYIYMIEVNNLSNTIKNLQDEGF", "aa_hexdigest": "cb5080d8780f592098625e3bf642a6c1", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5613.4003, "isoelectric_point": 5.985168647766113 }, "id": "NKIECMJPJD_67", "locus": "GCA000828735_00026" }, { "type": "cds", "sequence": "contig_1", "start": 14839, "stop": 14988, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08032.17" ], "nt": "ATGAATTATAATTTTGGCAAAAAAGCATTATTAGATGCAATTGGTAACAAACAATCAATTTCAAAAGTGTATTTATTATCAAAAAATTATGAGTTAATTAATTTAATTAATAAAAATAAAATTACTTATGAAATTGTTAATGAAGCATGA", "aa": "MNYNFGKKALLDAIGNKQSISKVYLLSKNYELINLINKNKITYEIVNEA", "aa_hexdigest": "257d76286064b4c4b47bc63c5661ba08", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08032.17", "name": "SpoU_sub_bind", "length": 51, "aa_cov": 0.9387755102040817, "hmm_cov": 1.0, "evalue": 1.3471142946780268e-07, "score": 27.41458511352539, "start": 4, "stop": 49 } ], "seq_stats": { "molecular_weight": 5635.488699999999, "isoelectric_point": 9.347996330261232 }, "id": "NKIECMJPJD_68", "locus": "GCA000828735_00027" }, { "type": "cds", "sequence": "contig_1", "start": 15068, "stop": 15559, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01406.26" ], "nt": "ATGCATTTTGGATTAGTTAATATTAATAATGAGAAAATGTCAAAATCTTTAAATAATTTTATTTTAGTAAAAGATTTATTAGCTGAATACGATTATCAAGTTGTGCGTTGGTTTTTTTATCAAGCAGATTATAAGCAACCAATTAAATTTTCGCATGAAATCATGAAGCAGAACGAAAAAGAAATTCTTAAAATTAAAAATGCTATTTATAACGCAAAAAACTATTTATACTTCAATAATCAACTAAAATCATTAACACAAATTGATCATTTTGAGCTTTTTGATGAACGCATTAATGATGATTTAGATTTTGTAGGTATTGTTGATCTAATTCATATTAGTGTTAAAAAAATAAATATTTTAATTAAAGAAAATAAGGATATGAATGAATTAAAACTGAATTTAACTCAATTATTATATATGTTAGATATATTGGGTATTAATTTTGTTGATTTGCATAATGATGAGAATTTAGCATTATTAAATACTTGA", "aa": "MHFGLVNINNEKMSKSLNNFILVKDLLAEYDYQVVRWFFYQADYKQPIKFSHEIMKQNEKEILKIKNAIYNAKNYLYFNNQLKSLTQIDHFELFDERINDDLDFVGIVDLIHISVKKINILIKENKDMNELKLNLTQLLYMLDILGINFVDLHNDENLALLNT", "aa_hexdigest": "b7afa85f1fa6bb8fde76edd219527084", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01406.26", "name": "tRNA-synt_1e", "length": 58, "aa_cov": 0.3558282208588957, "hmm_cov": 1.0, "evalue": 4.013575233008624e-18, "score": 61.326812744140625, "start": 1, "stop": 58 } ], "seq_stats": { "molecular_weight": 19447.252500000013, "isoelectric_point": 5.499934196472167 }, "id": "NKIECMJPJD_69", "locus": "GCA000828735_00028" }, { "type": "cds", "sequence": "contig_1", "start": 15560, "stop": 15688, "strand": "-", "frame": 2, "gene": null, "product": "tRNA synthetases class I catalytic domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A432FBA8" ], "nt": "TTGATCAACACTTATATTGGTGAACAAGTTAGTATTCATGGTGGTGGAATTGATTTAAAATTTCCCCACCATGAAAATGAAAATGCTCAAAATCAAGTTTTATATAATAAAAATTTAGCAAAAGTATGA", "aa": "MINTYIGEQVSIHGGGIDLKFPHHENENAQNQVLYNKNLAKV", "aa_hexdigest": "12f789fc56cb30128467d6e356cbfb35", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A432FBA8", "query_cov": 0.8333333333333334, "subject_cov": 0.8333333333333334, "identity": 0.5429999999999999, "score": 44.3, "evalue": 4.62e-05, "valid": false, "product": "tRNA synthetases class I catalytic domain-containing protein", "uniref50_id": "UniRef50_A0A432FBA8" }, "pscc": { "uniref50_id": "UniRef50_A0A432FBA8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A432FBA8" ], "product": "tRNA synthetases class I catalytic domain-containing protein" }, "genes": [], "id": "NKIECMJPJD_70", "locus": "GCA000828735_00029" }, { "type": "cds", "sequence": "contig_1", "start": 15767, "stop": 16300, "strand": "-", "frame": 2, "gene": "cysS", "product": "cysteine--tRNA ligase", "db_xrefs": [ "COG:COG0215", "COG:J", "EC:6.1.1.16", "GO:0004817", "GO:0005524", "GO:0005737", "GO:0005829", "GO:0006423", "GO:0008270", "KEGG:K01883", "SO:0001217", "UniRef:UniRef50_Q9PR21", "UniRef:UniRef90_Q9PR21" ], "nt": "ATGAAATTATATGATTCTTATTCTAATCAATTAGTAGAAATTAATGATGAATTAATTTCAATTTATAATTGTGGACCAACCGTTTATAATCATATCCATATTGGCAATGCTCGGCCTTTAATTACTATGGACGTGTTATATCGTTTTTTAAAAAAACATAATATCAAAACTAAATATGTTTTAAATATTACTGATATTGATGACAAAATCATTAATTATGCTCTAGCTAATAACCTAAAAGAATTAGAGGTTAGTGAATATTATTTTAATGAATATTTAAAAATTAAAAAAGCTTTAAATACACTAGAAATGATTAATCCTAAAGTATCAACACATATGGATAAAATTATTGATTATATCCAAAAATTGATTGATAAACAAGCAGGTTATTTTATTGGTGATGATGTTTATTTTGATACAAAAAAAGCTTTAAATTACGGACAATTATCGAAACGTGATTTAGAAAATGATATTGTTGGTATGCGTATTGAAAGCGCAGCTAATAAACACAATCCTAATGATTTTATTTTATGA", "aa": "MKLYDSYSNQLVEINDELISIYNCGPTVYNHIHIGNARPLITMDVLYRFLKKHNIKTKYVLNITDIDDKIINYALANNLKELEVSEYYFNEYLKIKKALNTLEMINPKVSTHMDKIIDYIQKLIDKQAGYFIGDDVYFDTKKALNYGQLSKRDLENDIVGMRIESAANKHNPNDFIL", "aa_hexdigest": "e792f8caccff44a245a3d12612c470ad", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [ "cysS" ], "truncated": "5-prime", "pseudogene": { "start": 15464, "stop": 16300, "observations": { "mutation": [ 15562, 15730, 15742, 15769 ], "stop": [ 15562, 15730, 15742, 15769 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PR21", "query_cov": 1.0, "subject_cov": 0.4059633027522936, "identity": 1.0, "score": 359.0, "evalue": 6.19e-122, "gene_start": 1, "gene_end": 177, "reference_start": 1, "reference_end": 177, "reference_sequence": "MKLYDSYSNQLVEINDELISIYNCGPTVYNHIHIGNARPLITMDVLYRFLKKHNIKTKYVLNITDIDDKIINYALANNLKELEVSEYYFNEYLKIKKALNTLEMINPKVSTHMDKIIDYIQKLIDKQAGYFIGDDVYFDTKKALNYGQLSKRDLENDIVGMRIESAANKHNPNDFILWKKTNKGIMWNTPWGIGRPGWHSECSCLINTYIGEQVSIHGGGIDLKFPHHENENAQNQVLYNKNLAKVWMHFGLVNINNEKMSKSLNNFILVKDLLAEYDYQVVRWFFYQADYKQPIKFSHEIMKQNEKEILKIKNAIYNAKNYLYFNNQLKSLTQIDHFELFDERINDDLDFVGIVDLIHISVKKINILIKENKDMNELKLNLTQLLYMLDILGINFVDLHNDENLALLNTWKNYVDKKDYVKADELRKQLINIGIL" }, "paralog": false, "identity": 0.9856115107913669, "score": 555.0, "evalue": 2.17e-201, "description": "Internal stop codon at 15562, 15730, 15742, 15769. Nonsense mutation around 15562, 15730, 15742, 15769.", "psc": { "uniref90_id": "UniRef90_Q9PR21", "gene": "cysS", "product": "cysteine--tRNA ligase", "ec_ids": [ "6.1.1.16" ], "uniref50_id": "UniRef50_Q9PR21", "cog_id": "COG0215", "cog_category": "J", "kegg_orthology_id": "K01883", "go_ids": [ "GO:0004817", "GO:0005524", "GO:0005737", "GO:0005829", "GO:0006423", "GO:0008270" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PR21", "EC:6.1.1.16", "UniRef:UniRef50_Q9PR21", "COG:COG0215", "COG:J", "KEGG:K01883", "GO:0004817", "GO:0005524", "GO:0005737", "GO:0005829", "GO:0006423", "GO:0008270" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PR21", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR21" ], "product": "Cysteine--tRNA ligase" } }, "id": "NKIECMJPJD_71", "locus": "GCA000828735_00030" }, { "type": "cds", "sequence": "contig_1", "start": 16608, "stop": 17009, "strand": "-", "frame": 1, "gene": null, "product": "MPN527 family putative ECF transporter permease subunit", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9X6S5", "UniRef:UniRef90_A0AAC9X6S5" ], "nt": "TTGAAGATAAATATAACAAAAGAAAAAACAATTTTTAAAAAAAATTATAAAAAAAAACATAGTATATATTATTCAACATCTAAAATTATTAAAGACTACACACTAAGTGCAGTAATGTTAGGTTTAGGAATTTTGGTTGCATATTTAAGTCACTTTATTCGTTTTAATTTCTTAGCTTTTGATTTTTCTTTGTTTACACTTGTTTATTTAATTTATAAAGTTAAATATCGCTTTGTATATTTAGTTACAATTTTATTAAGTTTAGCTAATTTATTGCACGGAAGTCCTTCATGGATCGGAACAATGGTTTTAAGTTTAAATAACTTATTTTTTGTTACTATGGTTATTCTTTTTAAAAAGTTATTTATTCCAAAAAGACCAAAATTTTATCTTAATTTATGA", "aa": "MKINITKEKTIFKKNYKKKHSIYYSTSKIIKDYTLSAVMLGLGILVAYLSHFIRFNFLAFDFSLFTLVYLIYKVKYRFVYLVTILLSLANLLHGSPSWIGTMVLSLNNLFFVTMVILFKKLFIPKRPKFYLNL", "aa_hexdigest": "f531acf65c3e813ae8585876f3e459f6", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 16314, "stop": 17009, "observations": { "mutation": [ 16433, 16532, 16610 ], "stop": [ 16433, 16532, 16610 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAC9X6S5", "query_cov": 1.0, "subject_cov": 0.5757575757575758, "identity": 0.97, "score": 239.0, "evalue": 2.26e-78, "gene_start": 1, "gene_end": 133, "reference_start": 1, "reference_end": 133, "reference_sequence": "MKINITKGKTIFKRNYKKKHSIYYSTSKIIKDYTLSAVMLGLGILIAYLSHFIRFNFLAFDFSLFTLVYLIYKVKYRFVYLVTILLSLANLLHGSPSWIGTMVLSLNNLFFVSMVILFKKLFIPKRPKFYLNLWVLVLSSFLTTVFNVIMNGILYTPLYWYTFKITPTLNFLEVQKIYEKHPTLFLLNIKSYWLGIIALYTSFNLIKNIIISFISLTILKIFADKKTIITY" }, "paralog": false, "identity": 0.9653679653679653, "score": 434.0, "evalue": 2.07e-157, "description": "Internal stop codon at 16433, 16532, 16610. Nonsense mutation around 16433, 16532, 16610.", "psc": { "uniref90_id": "UniRef90_A0AAC9X6S5", "product": "MPN527 family putative ECF transporter permease subunit", "uniref50_id": "UniRef50_A0AAC9X6S5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAC9X6S5", "UniRef:UniRef50_A0AAC9X6S5" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAC9X6S5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9X6S5" ], "product": "ECF transporter S component" } }, "id": "NKIECMJPJD_72", "locus": "GCA000828735_00031" }, { "type": "cds", "sequence": "contig_1", "start": 17267, "stop": 18226, "strand": "-", "frame": 2, "gene": "ligA", "product": "NAD-dependent DNA ligase LigA", "db_xrefs": [ "COG:COG0272", "COG:L", "EC:6.5.1.2", "GO:0003677", "GO:0003911", "GO:0005829", "GO:0006260", "GO:0006281", "GO:0006288", "GO:0046872", "SO:0001217", "UniRef:UniRef50_Q9PR23", "UniRef:UniRef90_Q9PR23" ], "nt": "ATGGTTGAATACGCTACTTTACATAACTTTGATTTTATTAAAGAAAAAGACATTCGAATTAATGATGAAATTAAAATTTATAAAGCAGGGGATGTAATTCCTTATGTTGATGGTGTTGATTTATCAAAACGTTTAGCAAATAGTGTACCTTATGAACCAATTATAAATTGCCCTTCATGCCAATCAGTTTTAGTACGTGAAAATGATGAAGTTGACCAACGCTGCTTAAATATTTATGGATGTAAAAAAATCAATATTGAAAAAATCGTTTATTTTGTTTCACGTAATTGCATGAATATTGAGGGAATGAGTGATGCCATTATTAATAAATTTTATGATGCTAATTTAATTAAAAACATTGCAGATTTATACTATTTGCAAAAACACAAAGAATTTATTTTAACTAGTGATTTTAAAATTAAAGAAAAAAGTTTTAGTAATTTAATTAATAGTATTAATAATTCAAAAAAATGTTCTTTAGAATTTTTATTAACAGCTTTTGGAATTCGTCATGTTGGACCAAATTTAGCTAAAAAAATAGCTAAACAATTTAAAACAATGACTGCTTTAATGCACGCTAATTTTGATGAATTAACTAATGTTGATGCATGTGGGGAAAAAGCAGCCTTATCATTAATTAATTGGTTTAATGATGAGCATAATGTTTCTTTAGTTAATCAATTACAACAAGTTGGTGTAAATATGGAATATATTGATGATTTTATTTATGATGATAACATCAATATAATTGATGAATATAAAAATAAAACTTTTGTTATTACTGGTTCATTTAGTATTAGCCGTGATGAAATTAAAACTATTTTAGAGAAATATTATCATGCAAAAGTTAAAAACAGTGTTTCTAAAAAAACAGATTATGTTTTAGTTGGCACTGAAGCGGGTACGAAATTAGAAAAAGCTAAATTATTAGGTGTGAAAATTATTGAAAATGAATTTTGA", "aa": "MVEYATLHNFDFIKEKDIRINDEIKIYKAGDVIPYVDGVDLSKRLANSVPYEPIINCPSCQSVLVRENDEVDQRCLNIYGCKKINIEKIVYFVSRNCMNIEGMSDAIINKFYDANLIKNIADLYYLQKHKEFILTSDFKIKEKSFSNLINSINNSKKCSLEFLLTAFGIRHVGPNLAKKIAKQFKTMTALMHANFDELTNVDACGEKAALSLINWFNDEHNVSLVNQLQQVGVNMEYIDDFIYDDNINIIDEYKNKTFVITGSFSISRDEIKTILEKYYHAKVKNSVSKKTDYVLVGTEAGTKLEKAKLLGVKIIENEF", "aa_hexdigest": "795b5edd976fd614ae58000e05993f80", "start_type": "ATG", "rbs_motif": null, "genes": [ "ligA" ], "truncated": "both", "pseudogene": { "start": 17246, "stop": 18526, "observations": { "mutation": [ 17269, 18355 ], "stop": [ 17269, 18355 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PR23", "query_cov": 1.0, "subject_cov": 0.4739970282317979, "identity": 1.0, "score": 635.0, "evalue": 1.13e-224, "gene_start": 1, "gene_end": 319, "reference_start": 348, "reference_end": 666, "reference_sequence": "MDKIKAKIDELKQKLDQWNYEYYVLDDPSVPDHVYDQTMRELIELENNYPQFKTNNSPSVKVGGFVSEKFNKVKHKRPMLSLSNAFNDDDLKKFDQDNQNASVDLKGYVVEPKIDGLSISIIYKNAKLHQAITRGDGINGEDVTSNILTIKDIPHYIDQKYKDYEIEVRGEVYMAFHDFYEMNDNLEESDKKFANPRNAAAGTLRSLDNSIVAERKLSAFMYYLVNAQELGIKTHYESIQFLRDNKFKVSDLIVKVDTINEVINQIDCYTKVRDKLSYMIDGIVIKINNLEVYDEIGYTSKFPKWAIAYKFPANVVSSQLLEIINDVGRTGKISYVAKIKPILLDGSMVEYATLHNFDFIKEKDIRINDEIKIYKAGDVIPYVDGVDLSKRLANSVPYEPIINCPSCQSVLVRENDEVDQRCLNIYGCKKINIEKIVYFVSRNCMNIEGMSDAIINKFYDANLIKNIADLYYLQKHKEFILTSDFKIKEKSFSNLINSINNSKKCSLEFLLTAFGIRHVGPNLAKKIAKQFKTMTALMHANFDELTNVDACGEKAALSLINWFNDEHNVSLVNQLQQVGVNMEYIDDFIYDDNINIIDEYKNKTFVITGSFSISRDEIKTILEKYYHAKVKNSVSKKTDYVLVGTEAGTKLEKAKLLGVKIIENEFWKKDNNF" }, "paralog": false, "identity": 0.9953051643192489, "score": 839.0, "evalue": 1.72e-307, "description": "Internal stop codon at 17269, 18355. Nonsense mutation around 17269, 18355.", "psc": { "uniref90_id": "UniRef90_Q9PR23", "gene": "ligA", "product": "NAD-dependent DNA ligase LigA", "ec_ids": [ "6.5.1.2" ], "uniref50_id": "UniRef50_Q9PR23", "cog_id": "COG0272", "cog_category": "L", "go_ids": [ "GO:0003677", "GO:0003911", "GO:0005829", "GO:0006260", "GO:0006281", "GO:0006288", "GO:0046872" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PR23", "EC:6.5.1.2", "UniRef:UniRef50_Q9PR23", "COG:COG0272", "COG:L", "GO:0003677", "GO:0003911", "GO:0005829", "GO:0006260", "GO:0006281", "GO:0006288", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PR23", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR23" ], "product": "DNA ligase" } }, "id": "NKIECMJPJD_73", "locus": "GCA000828735_00032" }, { "type": "cds", "sequence": "contig_1", "start": 18353, "stop": 19180, "strand": "-", "frame": 2, "gene": "ligA", "product": "NAD-dependent DNA ligase LigA", "db_xrefs": [ "COG:COG0272", "COG:L", "EC:6.5.1.2", "GO:0003677", "GO:0003911", "GO:0005829", "GO:0006260", "GO:0006281", "GO:0006288", "GO:0046872", "SO:0001217", "UniRef:UniRef50_Q9PR23", "UniRef:UniRef90_Q9PR23" ], "nt": "GTGCCAGACCATGTTTATGATCAAACAATGCGGGAGTTAATCGAACTTGAAAATAATTATCCTCAATTTAAAACTAACAATTCACCAAGTGTAAAGGTTGGCGGCTTTGTTTCAGAAAAATTTAATAAAGTAAAACATAAAAGACCAATGTTATCACTTTCAAATGCTTTTAATGATGATGATTTAAAAAAATTTGATCAAGATAATCAAAATGCTAGTGTCGATTTAAAAGGGTACGTAGTTGAACCTAAAATTGATGGTTTAAGCATTTCTATTATTTATAAAAATGCCAAATTACACCAGGCAATTACAAGAGGAGATGGGATTAATGGCGAAGATGTTACTTCCAATATTTTAACGATTAAAGACATTCCTCACTATATTGATCAAAAATATAAAGACTACGAAATTGAAGTTCGTGGTGAAGTCTATATGGCTTTTCATGATTTTTATGAAATGAATGATAATTTAGAAGAAAGTGATAAAAAATTTGCTAATCCAAGAAATGCTGCTGCTGGAACATTAAGAAGTCTAGATAATTCAATTGTTGCAGAACGTAAATTAAGTGCTTTTATGTATTATTTGGTTAATGCACAAGAACTAGGAATTAAAACGCATTATGAAAGTATTCAATTTTTAAGAGATAATAAATTTAAAGTTAGTGATTTAATTGTTAAAGTCGATACAATTAATGAAGTTATTAATCAAATTGATTGTTATACTAAAGTGCGTGATAAACTTTCATATATGATTGATGGTATTGTTATTAAAATTAATAATTTAGAAGTTTATGATGAGATTGGTTATACTTCTAAATTTCCTAAATGA", "aa": "MPDHVYDQTMRELIELENNYPQFKTNNSPSVKVGGFVSEKFNKVKHKRPMLSLSNAFNDDDLKKFDQDNQNASVDLKGYVVEPKIDGLSISIIYKNAKLHQAITRGDGINGEDVTSNILTIKDIPHYIDQKYKDYEIEVRGEVYMAFHDFYEMNDNLEESDKKFANPRNAAAGTLRSLDNSIVAERKLSAFMYYLVNAQELGIKTHYESIQFLRDNKFKVSDLIVKVDTINEVINQIDCYTKVRDKLSYMIDGIVIKINNLEVYDEIGYTSKFPK", "aa_hexdigest": "7a451a08b128203c34586d0e8f1bc665", "start_type": "GTG", "rbs_motif": null, "genes": [ "ligA" ], "truncated": "both", "pseudogene": { "start": 18050, "stop": 19267, "observations": { "mutation": [ 18355, 19216 ], "stop": [ 18355, 19216 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PR23", "query_cov": 1.0, "subject_cov": 0.4086181277860327, "identity": 0.996, "score": 544.0, "evalue": 1.7e-189, "gene_start": 1, "gene_end": 275, "reference_start": 30, "reference_end": 304, "reference_sequence": "MDKIKAKIDELKQKLDQWNYEYYVLDDPSVPDHVYDQTMRELIELENNYPQFKTNNSPSVKVGGFVSEKFNKVKHKRPMLSLSNAFNDDDLKKFDQDNQNASVDLKGYVVEPKIDGLSISIIYKNAKLHQAITRGDGINGEDVTSNILTIKDIPHYIDQKYKDYEIEVRGEVYMAFHDFYEMNDNLEESDKKFANPRNAAAGTLRSLDNSIVAERKLSAFMYYLVNAQELGIKTHYESIQFLRDNKFKVSDLIVKVDTINEVINQIDCYTKVRDKLSYMIDGIVIKINNLEVYDEIGYTSKFPKWAIAYKFPANVVSSQLLEIINDVGRTGKISYVAKIKPILLDGSMVEYATLHNFDFIKEKDIRINDEIKIYKAGDVIPYVDGVDLSKRLANSVPYEPIINCPSCQSVLVRENDEVDQRCLNIYGCKKINIEKIVYFVSRNCMNIEGMSDAIINKFYDANLIKNIADLYYLQKHKEFILTSDFKIKEKSFSNLINSINNSKKCSLEFLLTAFGIRHVGPNLAKKIAKQFKTMTALMHANFDELTNVDACGEKAALSLINWFNDEHNVSLVNQLQQVGVNMEYIDDFIYDDNINIIDEYKNKTFVITGSFSISRDEIKTILEKYYHAKVKNSVSKKTDYVLVGTEAGTKLEKAKLLGVKIIENEFWKKDNNF" }, "paralog": false, "identity": 0.9654320987654321, "score": 775.0, "evalue": 5.24e-283, "description": "Internal stop codon at 18355, 19216. Nonsense mutation around 18355, 19216.", "psc": { "uniref90_id": "UniRef90_Q9PR23", "gene": "ligA", "product": "NAD-dependent DNA ligase LigA", "ec_ids": [ "6.5.1.2" ], "uniref50_id": "UniRef50_Q9PR23", "cog_id": "COG0272", "cog_category": "L", "go_ids": [ "GO:0003677", "GO:0003911", "GO:0005829", "GO:0006260", "GO:0006281", "GO:0006288", "GO:0046872" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PR23", "EC:6.5.1.2", "UniRef:UniRef50_Q9PR23", "COG:COG0272", "COG:L", "GO:0003677", "GO:0003911", "GO:0005829", "GO:0006260", "GO:0006281", "GO:0006288", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PR23", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR23" ], "product": "DNA ligase" } }, "id": "NKIECMJPJD_74", "locus": "GCA000828735_00033" }, { "type": "cds", "sequence": "contig_1", "start": 19351, "stop": 20289, "strand": "-", "frame": 3, "gene": "uvrB", "product": "excinuclease ABC subunit UvrB", "db_xrefs": [ "COG:COG0556", "COG:L", "EC:3.1.25.-", "GO:0003677", "GO:0005515", "GO:0005524", "GO:0005737", "GO:0006289", "GO:0009380", "GO:0009381", "GO:0009432", "GO:0016787", "GO:0016887", "KEGG:K03702", "SO:0001217", "UniRef:UniRef50_P47319", "UniRef:UniRef90_Q9PR24" ], "nt": "ATGCATAATACAGACCGTAGTCGTAAACAAACATTAGTTGAATACGGATTTCGTTTACCTAGCGCTTTAGATAATCGGCCTTTAAATTATGATGAATTTAATAAAAAACTTTCCAAAACGATTTATGTTTCTGCCACACCAAACGATGAAGAAATTGCGTTATCAGATAATCATATAGTTAGTCAAATTGTTCGTCCTACAGGATTATTAGATCCAATTATTGAAATTAGAAAAACAGAACACCAAATTGATGATTTAATTAATGAATTGATGCTTTTAAAAAACAAGAATCAACGAGCATTTATTACTGTTATGACAATTAGAATGGCTGAGGATTTAACAAATTATTTAAATAATACAAAAATTAAAGCTGCTTATTTACATAATGAATTAAAAACACTAGAGCGTAGTGTTATTATCAATAAATTAAGAAAAGGAATTTATGATTGTGTTGTTGGAATTAATCTTTTAAGAGAAGGATTAGATGTTCCAGAAGTTGCAGGTGTTTTTATTTTTGATGCTGATAAACCTGGTTTTTTTAGAAGTGATAAATCTTTAATTCAAATTATTGGACGTGCAGCACGTAATGCTGATGGTAAAGTAATTATGTATGCTGATGTAATAACACAAGCCATGCAAACCGCAATCAATGAAACAAAACGACGACGTGAAATTCAATTAGCTTTTAATTTAAAACATAATATTATCCCTAAAACAATTATTAAACCAATTCACGAAGATTTAAGTGGCCATGATTATAAACAAAATGCGGAATTATATGCTGCAAAAGCTTCAAAAAACGAGTACAATCAAAAAATCAAAGAATTAAAGAAAAAAATGGAAGAAGCTGCAAAAAAACGTGAATATGAAGTTGCTGCTCAATATCGCGATATGATTGTTGAATTAGAAGCCATTAAGCAAAGTGTAAAAAGCAAATAA", "aa": "MHNTDRSRKQTLVEYGFRLPSALDNRPLNYDEFNKKLSKTIYVSATPNDEEIALSDNHIVSQIVRPTGLLDPIIEIRKTEHQIDDLINELMLLKNKNQRAFITVMTIRMAEDLTNYLNNTKIKAAYLHNELKTLERSVIINKLRKGIYDCVVGINLLREGLDVPEVAGVFIFDADKPGFFRSDKSLIQIIGRAARNADGKVIMYADVITQAMQTAINETKRRREIQLAFNLKHNIIPKTIIKPIHEDLSGHDYKQNAELYAAKASKNEYNQKIKELKKKMEEAAKKREYEVAAQYRDMIVELEAIKQSVKSK", "aa_hexdigest": "292305a417d2c364a71f362365bee818", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002024C376", "query_cov": 0.9871794871794872, "subject_cov": 0.9871794871794872, "identity": 0.5710000000000001, "score": 332.0, "evalue": 1.2e-110, "valid": false, "product": "helicase-related protein", "uniref50_id": "UniRef50_A0A519SUY2", "kegg_orthology_id": "K03702" }, "genes": [ "uvrB" ], "truncated": "3-prime", "pseudogene": { "start": 19351, "stop": 20589, "observations": { "mutation": [ 20340 ], "stop": [ 20340 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PR24", "query_cov": 1.0, "subject_cov": 0.46846846846846846, "identity": 1.0, "score": 600.0, "evalue": 6.2e-211, "gene_start": 1, "gene_end": 312, "reference_start": 355, "reference_end": 666, "reference_sequence": "MDDFKKFELISDYKPAGDQQKAIDEMVNNINQGIQRQVLLGATGTGKTFSVANVIAKTQLKTLVLAHNKTLAAQLFAELKEMFPHNKVEYFVSYFDYYQPEAYKPITDTYIEKDSVTNAEIEMMRLSTINSLATRNDVIVVASVACIYASVSPIEFTKKNLYLHVGELIEFEQIKYKLVQLGYERKDYDLVPGTFRIRGDLIEIMSGYSDKFKIRISMFGNEVESIDLCDPITNNIISKEQRFILRSASEYIFDDSRLAIAIENIKNELDERVKFFLKNNQLIEAQRIEQRTKQDLESIVEFGFCSGVENYYRHLEHREPFQTPWTIFDFFSYNNQDWLLIVDESHISLPQVKGMHNTDRSRKQTLVEYGFRLPSALDNRPLNYDEFNKKLSKTIYVSATPNDEEIALSDNHIVSQIVRPTGLLDPIIEIRKTEHQIDDLINELMLLKNKNQRAFITVMTIRMAEDLTNYLNNTKIKAAYLHNELKTLERSVIINKLRKGIYDCVVGINLLREGLDVPEVAGVFIFDADKPGFFRSDKSLIQIIGRAARNADGKVIMYADVITQAMQTAINETKRRREIQLAFNLKHNIIPKTIIKPIHEDLSGHDYKQNAELYAAKASKNEYNQKIKELKKKMEEAAKKREYEVAAQYRDMIVELEAIKQSVKSK" }, "paralog": false, "identity": 0.9975728155339806, "score": 808.0, "evalue": 2.98e-295, "description": "Internal stop codon at 20340. Nonsense mutation around 20340.", "psc": { "uniref90_id": "UniRef90_Q9PR24", "gene": "uvrB", "product": "excinuclease ABC subunit UvrB", "ec_ids": [ "3.1.25.-" ], "uniref50_id": "UniRef50_P47319", "cog_id": "COG0556", "cog_category": "L", "kegg_orthology_id": "K03702", "go_ids": [ "GO:0003677", "GO:0005515", "GO:0005524", "GO:0005737", "GO:0006289", "GO:0009380", "GO:0009381", "GO:0009432", "GO:0016787", "GO:0016887" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PR24", "EC:3.1.25.-", "UniRef:UniRef50_P47319", "COG:COG0556", "COG:L", "KEGG:K03702", "GO:0003677", "GO:0005515", "GO:0005524", "GO:0005737", "GO:0006289", "GO:0009380", "GO:0009381", "GO:0009432", "GO:0016787", "GO:0016887" ] }, "pscc": { "uniref50_id": "UniRef50_P47319", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47319" ], "product": "UvrABC system protein B" } }, "id": "NKIECMJPJD_75", "locus": "GCA000828735_00034" }, { "type": "cds", "sequence": "contig_1", "start": 20338, "stop": 21351, "strand": "-", "frame": 3, "gene": null, "product": "Excinuclease ABC subunit B", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PB45" ], "nt": "ATGGATGATTTTAAAAAATTTGAATTAATAAGCGATTATAAACCAGCTGGTGACCAACAAAAGGCAATTGATGAAATGGTTAATAATATTAATCAAGGTATTCAACGACAAGTATTATTAGGGGCAACAGGAACAGGAAAAACATTTAGTGTGGCTAATGTAATTGCAAAAACCCAATTAAAGACTTTAGTTCTTGCTCATAATAAAACATTAGCTGCACAATTATTTGCTGAACTTAAAGAAATGTTTCCTCATAATAAAGTTGAATATTTTGTTTCATATTTTGATTATTATCAACCAGAAGCTTATAAACCTATAACTGATACTTATATTGAAAAAGATTCAGTAACAAATGCAGAAATTGAGATGATGCGTTTATCAACTATTAATTCATTGGCTACAAGAAATGATGTCATTGTTGTTGCTTCTGTTGCATGTATTTATGCATCTGTTTCTCCTATAGAATTTACTAAAAAGAATTTATATTTACATGTTGGTGAATTAATCGAATTTGAACAAATTAAATATAAATTAGTTCAATTAGGTTATGAACGAAAAGATTATGATTTAGTGCCAGGAACATTTAGGATTCGTGGTGATTTAATTGAAATTATGTCTGGATATAGTGATAAATTTAAAATAAGAATATCAATGTTTGGAAATGAAGTTGAATCAATTGATCTTTGCGATCCAATTACTAATAATATTATTTCTAAAGAACAAAGGTTTATTTTACGAAGTGCAAGTGAATATATTTTTGATGATTCGCGTTTAGCAATAGCGATTGAAAATATTAAAAATGAACTAGATGAACGAGTTAAATTTTTTCTAAAAAACAATCAATTAATTGAGGCGCAACGGATTGAGCAACGAACTAAACAAGATCTTGAAAGTATTGTAGAGTTTGGTTTTTGTAGTGGAGTTGAAAATTATTATCGTCATCTTGAACATCGTGAACCTTTTCAAACACCATGGACAATTTTTGATTTTTTTTCATATAACAATCAAGATTGA", "aa": "MDDFKKFELISDYKPAGDQQKAIDEMVNNINQGIQRQVLLGATGTGKTFSVANVIAKTQLKTLVLAHNKTLAAQLFAELKEMFPHNKVEYFVSYFDYYQPEAYKPITDTYIEKDSVTNAEIEMMRLSTINSLATRNDVIVVASVACIYASVSPIEFTKKNLYLHVGELIEFEQIKYKLVQLGYERKDYDLVPGTFRIRGDLIEIMSGYSDKFKIRISMFGNEVESIDLCDPITNNIISKEQRFILRSASEYIFDDSRLAIAIENIKNELDERVKFFLKNNQLIEAQRIEQRTKQDLESIVEFGFCSGVENYYRHLEHREPFQTPWTIFDFFSYNNQD", "aa_hexdigest": "154fad3cf5a061451ea69eb5fa820738", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00056E9490", "query_cov": 0.9792284866468842, "subject_cov": 0.9348441926345609, "identity": 0.742, "score": 488.0, "evalue": 3.77e-171, "valid": false, "product": "DEAD/DEAH box helicase family protein", "uniref50_id": "UniRef50_A0A3B0PB45", "kegg_orthology_id": "K03702" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0PB45", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PB45" ], "product": "Excinuclease ABC subunit B" }, "genes": [], "id": "NKIECMJPJD_76", "locus": "GCA000828735_00035" }, { "type": "cds", "sequence": "contig_1", "start": 21702, "stop": 22892, "strand": "-", "frame": 1, "gene": "secA", "product": "preprotein translocase subunit SecA", "db_xrefs": [ "COG:COG0653", "COG:U", "EC:7.4.2.8", "GO:0005524", "GO:0005829", "GO:0005886", "GO:0006605", "GO:0006886", "GO:0008564", "GO:0017038", "GO:0031522", "GO:0043952", "GO:0065002", "SO:0001217", "UniRef:UniRef50_Q9PR25", "UniRef:UniRef90_Q9PR25" ], "nt": "ATGGAAGGAAGAAGTTATAGCGCTGGATTACAACAAGCAATTCAAGCTAAAGAACAAATTAAAATTGAACCCGAAAATTTAACAGTTGCCACAATCACTTATCAATCATTATTTCGTTTATATAAAAAATTAGCAGCAGTTTCAGGAACAGCTATTACTGAAGCAGAAGAATTTTTAAATATTTATAATATGGTTGTTGTTACAATCCCAACAAATAAACCAATTAAAAGAATTGACCATCCTGATTATGTTTTTGATAATAAAAGAACAAAATGGAAATATGTAATTGCTGATGTTATTCGTCGTCATGAAAATGGCCAACCAATTTTAATTGGAACTGCTAGTGTTGAAGATTCAGAAATTTTACATCAATTACTAGAACGAGTCAATATTCCTCACGAAGTTTTAAATGCTAAAAATCACGCACGCGAAGCTGAAATTATTGCTTGTGCAGGAGAGTATAAAGCAGTTACAATAGCAACAAATATGGCAGGCCGTGGGACTGATATTAAATTATCTCCAGAATCATTAGAAGCTGGAGGATTATGTGTTATTGGAACCGAACGAAGTGATTCACGTAGAATTGATAATCAGTTAAGAGGACGTGCTGGACGACAAGGTGATATTGGCGAATCACGTTTTTTTATTTCAATGGAAGATACTTTATTTAGTCGTTTTGCAACCGATAATTTAGCAAAAGCTGATGATAAATTAAGTGAAGATGTTATTTCAACAAAATTCTTTACACGATTGCTAAATAATACACAAAAAAAAGTTGAGTCGTTAAACTATGACACAAGAAAAAATCTAATTGATTATGATCATGTTTTAAGTAATCAACGCGAATTAATTTACAAACAACGGGATAAAATTCTTATTTCATCAGATAATAAAGATATTTTATATCGAATGTTAGATAGTGTAATTGATGATTTAATTTATCAATCACACAATAAACCAAATGAAGACATTATTGACATAAAAAAATTAATTGATTTAGCAACTCAAAACATTTTTTATGACAATTATTTAAATCAGGACGAATATTATGGGCTTAAGTTTGAACAAATTAAAACTAAATTAAAAAAAGACTGTATTAATTTTTTTGAGCAAAAAGAACAACTAATGACACCGACAATTTTTAATCAAATCTTATCAGAAATTATGATTAGTAATATTGATGAAGAATGA", "aa": "MEGRSYSAGLQQAIQAKEQIKIEPENLTVATITYQSLFRLYKKLAAVSGTAITEAEEFLNIYNMVVVTIPTNKPIKRIDHPDYVFDNKRTKWKYVIADVIRRHENGQPILIGTASVEDSEILHQLLERVNIPHEVLNAKNHAREAEIIACAGEYKAVTIATNMAGRGTDIKLSPESLEAGGLCVIGTERSDSRRIDNQLRGRAGRQGDIGESRFFISMEDTLFSRFATDNLAKADDKLSEDVISTKFFTRLLNNTQKKVESLNYDTRKNLIDYDHVLSNQRELIYKQRDKILISSDNKDILYRMLDSVIDDLIYQSHNKPNEDIIDIKKLIDLATQNIFYDNYLNQDEYYGLKFEQIKTKLKKDCINFFEQKEQLMTPTIFNQILSEIMISNIDEE", "aa_hexdigest": "8313da75dcca15d557a63992a47a03da", "start_type": "ATG", "rbs_motif": null, "genes": [ "secA" ], "truncated": "5-prime", "pseudogene": { "start": 21399, "stop": 23192, "observations": { "mutation": [ 21569, 21704 ], "stop": [ 21569, 21704 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PR25", "query_cov": 1.0025252525252526, "subject_cov": 0.47205707491082044, "identity": 0.9570000000000001, "score": 740.0, "evalue": 3.87e-262, "gene_start": 1, "gene_end": 396, "reference_start": 326, "reference_end": 722, "reference_sequence": "MNLISKISPQNRILNHARLIAEEVLKKEDEYTHFSDQELINKSDDIIEYLANNNPLDDRLVESLCIIREVIYRVHNKRAFKVQLIGAIIVYFGDFAEMMTGEGKTLTLVLVAYLNALYKKGVHMVTVNEYLVKVGAEFATPALNFLNMSVGQITANMNEYEKRNNYNCDITYTTNSELGFDYLRDNMVTNYNSKVQRGLWFAIVDEGDSVLIDEARTPLIISGEPQEEIGNYVKADRFVKTLYPQDFTLDPESQSVALTESGVEKAQKFFNTKNYYNFENSDIIHKVTNALRANFTFFNGREYIVKKDDEGEDVIALVDQSTGRIMEGRSYSAGLQQAIQAKEQIKIEPENLTVATITYQSLFRLYKKLAAVSGTAITEVEEFLNIYNMVVVTIPTNKPIRRIDHPDYVFDNKRTKWKYVIADVIRRHENGQPILIGTASVEDSEILHQLLERVNIPHEVLNAKNHAREAEIVARAGEYKAVTIATNMAGRGTDIKLSPESLEAGGLCVIGTERSDSRRIDNQLRGRAGRQGDIGESRFFISMEDTLFSRFATDNLAKADDKLSEDVISTKFFTRLLNNTQKKVESLNYDTRKNLIDYDHVLSNQRELIYKQRDKILVSSDNKDILYRMLDSVIDDIIYQSHNEPNEDIIDVKKLIDLATQNIFYDNYLNHDEYYGLDLIDEIKTKLKNDCISFFEQKEQLMTPGIFNQILSEIMISNIDEEWTKHLDVTSKIREGVNLRAYEQKAPLNIYVEDSDKLFEKLKHDVAWKTVCSIGKINYVHQEYDEVNNEFIINDNEIIDNDNVIDFENTDHSLISEQEIEDSLVNIDELNDQNTKNENND" }, "paralog": false, "identity": 0.919732441471572, "score": 1090.0, "evalue": 0.0, "description": "Internal stop codon at 21569, 21704. Nonsense mutation around 21569, 21704.", "psc": { "uniref90_id": "UniRef90_Q9PR25", "gene": "secA", "product": "preprotein translocase subunit SecA", "ec_ids": [ "7.4.2.8" ], "uniref50_id": "UniRef50_Q9PR25", "cog_id": "COG0653", "cog_category": "U", "go_ids": [ "GO:0005524", "GO:0005829", "GO:0005886", "GO:0006605", "GO:0006886", "GO:0008564", "GO:0017038", "GO:0031522", "GO:0043952", "GO:0065002" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PR25", "EC:7.4.2.8", "UniRef:UniRef50_Q9PR25", "COG:COG0653", "COG:U", "GO:0005524", "GO:0005829", "GO:0005886", "GO:0006605", "GO:0006886", "GO:0008564", "GO:0017038", "GO:0031522", "GO:0043952", "GO:0065002" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PR25", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR25" ], "product": "Protein translocase subunit SecA" } }, "id": "NKIECMJPJD_77", "locus": "GCA000828735_00036" }, { "type": "cds", "sequence": "contig_1", "start": 23268, "stop": 23867, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF07517.20" ], "nt": "ATGAATTTAATATCAAAAATATCCCCCCAAAACCGAATCTTAAATCGCGCTCGATTGATTGCTGAAGAAGTTTTAAAAAAAGAAGAAGAATACGAACATTTTAGCGATCAAGAATTAATTAATAAATCTGATGATATTATTGAATATTTAGCAAATAATAACCCATTAGATGATAAATTAGTAGAAGCATTATGCATTATTCGTGAAGTGATTTATCGAGTTCATAATAAAAGAGCTTTTAAAGTTCAAATTATTGGTGCAATTATTGTTTATTTTGGTGATTTTGCTGAAATGATGACTGGTGAGGGTAAAACATTAACTTTAGTTTTAGTTGCTTATTTAAATGCACTTTATAAAAAAGGTGTTCATATGGTAACTGTTAACGAATATCTAGTTAAAGTTGGAGCAGAATTTGCAACACCTGTTTTAAATTTTTTAAATATGTCTGTTGGCCAAATTACAGCTAATATGAATGAGTATGAAAAACGAAATAATTATAATTGTGATATCACATATACAACTAATTCAGAATTAGGCTTTGATTATTTACGTGATAATATGGTAACTAATTATGCTAATAAAGTTCAACGGGGCTTATGA", "aa": "MNLISKISPQNRILNRARLIAEEVLKKEEEYEHFSDQELINKSDDIIEYLANNNPLDDKLVEALCIIREVIYRVHNKRAFKVQIIGAIIVYFGDFAEMMTGEGKTLTLVLVAYLNALYKKGVHMVTVNEYLVKVGAEFATPVLNFLNMSVGQITANMNEYEKRNNYNCDITYTTNSELGFDYLRDNMVTNYANKVQRGL", "aa_hexdigest": "bab72ca190832d102e01c33dc36407f1", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07517.20", "name": "SecA_DEAD", "length": 192, "aa_cov": 0.964824120603015, "hmm_cov": 1.0, "evalue": 1.3337762909283493e-69, "score": 230.1531219482422, "start": 8, "stop": 199 } ], "seq_stats": { "molecular_weight": 22903.04580000001, "isoelectric_point": 5.486577033996581 }, "id": "NKIECMJPJD_78", "locus": "GCA000828735_00037" }, { "type": "cds", "sequence": "contig_1", "start": 23896, "stop": 24786, "strand": "-", "frame": 3, "gene": "lolD", "product": "ABC Transporter", "db_xrefs": [ "COG:COG1136", "COG:M", "SO:0001217", "UniRef:UniRef50_Q9PR26", "UniRef:UniRef90_Q9PR26" ], "nt": "ATGAGAAATAAAAAAGAAAATGATAATGTTATTAATCCATTATCTAATCCAGATGAGAACAAAAAAATTAATATAAAAGACTTATCAAAAGATGAGCGTAAAGAACTTAAAAAACGAATTAAAGAAGTTGAAAAATTAAACAGAAATCACAAAGGTGACATTGATATGCCATCACGTGATCCAAATAATATTATTGAACTTCGTGATGTTAAAAAAATTTTCACAAATGGCTATTTAATTAATGAAACACTCAAAGGTGTTAATTTAGATATTCGTAAAGGTGAATTTGTAATTATTTTAGGACCATCAGGTTCTGGTAAAACAACATTAATGAACATTATGTCGGGACTTGATCGTGCAACTGATGGTGATGTTCGTGTTTGTGATAAACAACTAATTAATATGAATCAAAATAAATTAACTGATTTTCGTAAAGAATATATTGGATTTGTTTTTCAACAATATGGATTATTACCAACACTATCTGTAGAGGAAAATGTAGAGATTGGTGCTGATTTACAAGTTGATAAAAAACGCCGTATTAAACCACAAGATGCATTAAAAGCAGTAGGGATGCTTGATTACGCAAAAAAATTTCCACATGAATTATCTGGTGGTCAGCAACAACGAGTCTCAATAGCCCGTGCTTTAGCTAAAAATCCTATTATTTTATTTGGCGATGAACCAACTGGTGCTGTTGATGAAACAATGTCAAAAATTATTTTAAAAGAATTTGTAAAAGTTAACCAGGAATTAAAGACAACGGTAATTATTGTTACCCACAACCCAATTTTTGCTGAACTTGGAACTTTAGTTATTAAGGTTAAAGATGGTAATATTAATGAATTAATTCGTAATGATCATCCAAAATCAGTTGACGAATTAAAATGA", "aa": "MRNKKENDNVINPLSNPDENKKINIKDLSKDERKELKKRIKEVEKLNRNHKGDIDMPSRDPNNIIELRDVKKIFTNGYLINETLKGVNLDIRKGEFVIILGPSGSGKTTLMNIMSGLDRATDGDVRVCDKQLINMNQNKLTDFRKEYIGFVFQQYGLLPTLSVEENVEIGADLQVDKKRRIKPQDALKAVGMLDYAKKFPHELSGGQQQRVSIARALAKNPIILFGDEPTGAVDETMSKIILKEFVKVNQELKTTVIIVTHNPIFAELGTLVIKVKDGNINELIRNDHPKSVDELK", "aa_hexdigest": "41a624662403dd7d52180f918d8a91ea", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_Q9PR26", "query_cov": 1.0, "subject_cov": 0.9866666666666667, "identity": 0.9590000000000001, "score": 540.0, "evalue": 2.12e-193, "valid": true, "gene": "LolD", "product": "ABC Transporter", "uniref50_id": "UniRef50_Q9PR26", "cog_id": "COG1136", "cog_category": "M" }, "pscc": { "uniref50_id": "UniRef50_Q9PR26", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR26" ], "product": "ABC Transporter" }, "genes": [ "lolD" ], "id": "NKIECMJPJD_79", "locus": "GCA000828735_00038" }, { "type": "cds", "sequence": "contig_1", "start": 24925, "stop": 26160, "strand": "+", "frame": 1, "gene": "tyrS", "product": "tyrosine--tRNA ligase", "db_xrefs": [ "COG:COG0162", "COG:J", "EC:6.1.1.1", "GO:0000166", "GO:0003723", "GO:0004831", "GO:0005524", "GO:0005829", "GO:0006437", "KEGG:K01866", "SO:0001217", "UniRef:UniRef50_Q9PR27", "UniRef:UniRef90_Q9PR27" ], "nt": "ATGCATAGTTTAATTAAGGATTTAAAAGCACGAAACTTAATCAATAATATAACTAATGAAGAAAAATTAATAAAAGCTTTAGAGAAAAATAAAGGAATTTATGTAGGTTTTGATCCTTCAGCTGATAGTTTACACTTAGGTAATTATATTATGATAATGTTATTAAAACGCTTTCGTTTATATAACATTAAAACATTAGCGCTTGTTGGTGGTGCTACAGGAATGATTGGTGATCCAAGTGGTAAATCCGCTGAACGTAATTTATTAGATAAAACAATTTTAGAAAAAAATATTGCAAAAATAAAATTTCAACTTGAAAAATTTACTAATTCTCAAGTTATTAATAATTATGTTTTTTATGAAAATATGACTTTTTTAGATTTTTTGCGTGATGTTGGAAAGTTAATCAACATTAATTATTTATTAGAAAAAGAAATTATTAATTCTCGTTTATCTGTTGGTATTTCATATACAGAATTTAGTTATAATTTGCTACAAGGTTATGATTTTTTACAATTATATAAAAATGATAACATAGCCATTCAAGCTGGTGGTAGTGATCAATGGGGTAACATTACAACTGGAATTGAAATGATTCGAAAAAATTTAGGTGATGACAATATCGCTTGTGGATTGACAATTAATTTATTAACAAATTCAGAAGGTAAAAAATTTGGTAAAAGTGAAAAAGGTGCTATTTATTTAGACGAAAATAAATCAACTGTTTATGAAATGTATCAGTTTTTAATTAATCAAAGCGATGCTGATGTAGAAAAATTACTTAATTTTTTAACATTAATTGATGTTGAAGAAATAAAAAAAATAATGCAAGCTCATAAAAATAATCCCGCTTTACGAGTTGCACAAAAAGCATTGGCTAAAGCTGTTGTTGTTGATATACATGGACAACAAAAATATGAACAAGCATTACATATTAGCGAAGTACTATTTAATGGTAGTATTAGTACATTAAATCAAGAAGAATTAGAAATCGCAATTAAGAGTTTACCTGCAACAAAATTAGACAAAGATGAAATTAAAATAATTGATTTATTAAATTTAGCTAATATTTCATCAAGTAACCGTATAGCACGAGATTTTTTAAATACAGGATCAATTTTAATTAATGATATTAAAATAAATGATGAAAATTTTTTAGTCAAGAAACAAGATGCTATTAATCAAAAATTTAGTATTATTAGAAAAGGTAAACGAAATTATTTTTTAATTTTATGA", "aa": "MHSLIKDLKARNLINNITNEEKLIKALEKNKGIYVGFDPSADSLHLGNYIMIMLLKRFRLYNIKTLALVGGATGMIGDPSGKSAERNLLDKTILEKNIAKIKFQLEKFTNSQVINNYVFYENMTFLDFLRDVGKLININYLLEKEIINSRLSVGISYTEFSYNLLQGYDFLQLYKNDNIAIQAGGSDQWGNITTGIEMIRKNLGDDNIACGLTINLLTNSEGKKFGKSEKGAIYLDENKSTVYEMYQFLINQSDADVEKLLNFLTLIDVEEIKKIMQAHKNNPALRVAQKALAKAVVVDIHGQQKYEQALHISEVLFNGSISTLNQEELEIAIKSLPATKLDKDEIKIIDLLNLANISSSNRIARDFLNTGSILINDIKINDENFLVKKQDAINQKFSIIRKGKRNYFLIL", "aa_hexdigest": "6d8ada0e833d7e60a0090ef3dea517f5", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_Q9PR27", "query_cov": 1.0, "subject_cov": 0.9903614457831326, "identity": 1.0, "score": 785.0, "evalue": 1.88e-286, "valid": true, "gene": "tyrS", "product": "tyrosine--tRNA ligase", "ec_ids": [ "6.1.1.1" ], "uniref50_id": "UniRef50_Q9PR27", "cog_id": "COG0162", "cog_category": "J", "kegg_orthology_id": "K01866", "go_ids": [ "GO:0000166", "GO:0003723", "GO:0004831", "GO:0005524", "GO:0005829", "GO:0006437" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PR27", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR27" ], "product": "Tyrosine--tRNA ligase" }, "genes": [ "tyrS" ], "id": "NKIECMJPJD_80", "locus": "GCA000828735_00039" }, { "type": "ncRNA", "class": null, "sequence": "contig_1", "start": 26198, "stop": 26295, "strand": "+", "gene": "Bacteria_small_SRP", "product": "Bacterial small signal recognition particle RNA", "score": 58.4, "evalue": 3e-07, "db_xrefs": [ "RFAM:RF00169", "SO:0000655" ], "nt": "TATATATGTCATTACAATATTAAGTAGCGAACCTTGTCAGGCCAGAGATGGAGCAGCAATAGCAATATCTTTTTATGTGTGATGACATATATTAATGT", "id": "NKIECMJPJD_40", "locus": "GCA000828735_00040" }, { "type": "cds", "sequence": "contig_1", "start": 26417, "stop": 27040, "strand": "+", "frame": 2, "gene": "upp", "product": "uracil phosphoribosyltransferase", "db_xrefs": [ "COG:COG0035", "COG:F", "EC:2.4.2.9", "GO:0000287", "GO:0004845", "GO:0005525", "GO:0006223", "GO:0044206", "RefSeq:WP_045470308.1", "SO:0001217", "UniParc:UPI00045633DF", "UniRef:UniRef100_UPI00045633DF", "UniRef:UniRef50_P70881", "UniRef:UniRef90_Q9PR28" ], "nt": "ATGCATAAAATAATTAATCACCCATTAATTAAAGATAAACTAACACGTATACGAAAAGTTTCAACAGTTTCTACTGTTTTTCGTACAAATTTAGAAGAACTTACACAATTGATGGTTTATGAAGCAACAAAAGATTTAGAGTTAAATGAAATAGAAATTGAAACTCCTGTTGTTAAGGTTGCAAAAGGTTATAAATTAAAAAATAAAATTTGTTTAATACCTATTTTGCGCGCTGGAATTGGAATGGTTGATGGTGTTAAAAGTTTAATTCCAACAGCCACAATCGGTCATATCGGTTTATATCGTAATGAACAAACACTAAAACCAGTAGAATATTTTAAAAAATTTCCTAAAAATATTTCTGAATCAGATGTTATTATTTTAGATCCAATGTTAGCAACAGGAGGTAGTGTTGTTGAAGCTGTTAATATTATTAAAAAATACAATCCAAAATCCATTAAATTTGTATGTATTGTTGCAGCTCCAGAAGGTCTTAAATATGTTCAAGAAGTTCATCCTGAAGTTGATGTTTATATCGCAGCATTAGATGATAAATTAAATGAAAATGGATATATCACTCCTGGTTTAGGTGATGCTGGTGATCGAATTTTCGGTACTAAATAA", "aa": "MHKIINHPLIKDKLTRIRKVSTVSTVFRTNLEELTQLMVYEATKDLELNEIEIETPVVKVAKGYKLKNKICLIPILRAGIGMVDGVKSLIPTATIGHIGLYRNEQTLKPVEYFKKFPKNISESDVIILDPMLATGGSVVEAVNIIKKYNPKSIKFVCIVAAPEGLKYVQEVHPEVDVYIAALDDKLNENGYITPGLGDAGDRIFGTK", "aa_hexdigest": "a537599143719c9533c0e0e154e69ef3", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI00045633DF", "ncbi_nrp_id": "WP_045470308.1", "uniref100_id": "UniRef100_UPI00045633DF", "db_xrefs": [ "SO:0001217", "UniParc:UPI00045633DF", "RefSeq:WP_045470308.1", "UniRef:UniRef100_UPI00045633DF" ] }, "ips": { "uniref100_id": "UniRef100_UPI00045633DF", "uniref90_id": "UniRef90_Q9PR28" }, "psc": { "uniref90_id": "UniRef90_Q9PR28", "gene": "upp", "product": "uracil phosphoribosyltransferase", "ec_ids": [ "2.4.2.9" ], "uniref50_id": "UniRef50_P70881", "cog_id": "COG0035", "cog_category": "F", "go_ids": [ "GO:0000287", "GO:0004845", "GO:0005525", "GO:0006223", "GO:0044206" ] }, "pscc": { "uniref50_id": "UniRef50_P70881", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P70881" ], "product": "Uracil phosphoribosyltransferase" }, "genes": [ "upp" ], "id": "NKIECMJPJD_81", "locus": "GCA000828735_00041" }, { "type": "tRNA", "sequence": "contig_1", "start": 27105, "stop": 27178, "strand": "-", "gene": "trnG", "product": "tRNA-Gly(gcc)", "amino_acid": "Gly", "anti_codon": "gcc", "score": 72.5, "nt": "GCAGATGTAGTTCAATGGTAGAACGCAACCTTGCCAAGGTCGAGACGCGAGTTCGATTCTCGTCATCTGCTCCA", "db_xrefs": [ "SO:0000260" ], "anti_codon_pos": [ 27144, 27146 ], "id": "NKIECMJPJD_30", "locus": "GCA000828735_00042" }, { "type": "cds", "sequence": "contig_1", "start": 27340, "stop": 27915, "strand": "-", "frame": 3, "gene": null, "product": "Phosphoglycolate phosphatase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR29" ], "nt": "ATGACCAAAAAACTGATTGTTTTTGATTTTGATGGTACAATTATGCACACGCATACAACTATGAGCCTATCTATTATTGGTGTTTTAGAATTTTATAATCATCATATACCAAGTTTAAAAGAAATGAACGATCTGTTAGGTAATCTTTCTATGGTAAATATTTTTAAAAAGTATGCTAAACACGATCTTTTAAATATCGAAATTGAAATGATGATTTCTAAATATTATGAAATATATGAATCTTCGCTTTTTATGATCCATAGTTATTTTTTTGATGGTATTTTAGACCTAATCAAAAAATTAAAATCACTTAATAATAATGTTAAATTAGCAATTTTATCAAATAAACGATCTTCTTTATTAGCAACAATGGTTGATTATTATAATTTACGACCTTATTTTGATTATATTTTTGGTGCTGAAGATGTCGAACAAATGAAGCCAGATCCTAGTGGTTTACTAAAAATTATGAACAACTGTAATGTTGATCATAAAAATACTTTATTGATTGGTGATAGTATAGCTGATTTAAAAGCAGCTATAAATGCTAATTGTCATTTTATTTTAGTTAATTGA", "aa": "MTKKLIVFDFDGTIMHTHTTMSLSIIGVLEFYNHHIPSLKEMNDLLGNLSMVNIFKKYAKHDLLNIEIEMMISKYYEIYESSLFMIHSYFFDGILDLIKKLKSLNNNVKLAILSNKRSSLLATMVDYYNLRPYFDYIFGAEDVEQMKPDPSGLLKIMNNCNVDHKNTLLIGDSIADLKAAINANCHFILVN", "aa_hexdigest": "c3293af79bf3974e8ddfde5fe6522b21", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "psc": { "uniref90_id": "UniRef90_A0AA86YSP4", "query_cov": 1.0, "subject_cov": 0.8488888888888889, "identity": 0.853, "score": 332.0, "evalue": 3.96e-114, "valid": false, "product": "Phosphoglycolate phosphatase", "uniref50_id": "UniRef50_Q9PR29" }, "pscc": { "uniref50_id": "UniRef50_Q9PR29", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR29" ], "product": "Phosphoglycolate phosphatase" }, "genes": [], "id": "NKIECMJPJD_82", "locus": "GCA000828735_00043" }, { "type": "cds", "sequence": "contig_1", "start": 27923, "stop": 28270, "strand": "-", "frame": 2, "gene": "folA", "product": "Dihydrofolate reductase", "db_xrefs": [ "COG:COG0262", "COG:H", "EC:1.5.1.3", "GO:0004146", "GO:0005829", "GO:0006730", "GO:0046452", "GO:0046654", "GO:0046655", "GO:0050661", "KEGG:K00287", "SO:0001217", "UniRef:UniRef50_Q9PR30", "UniRef:UniRef90_Q9PR30" ], "nt": "ATGGGTAAAAACACTTTTGATTCATTAGATCAAAAACCATTAAATAATCGAACAAATATCATTTTTTCTTCAATTATGCAAACACCAGAAGACGAATCGTATTTTGTGACAAATGATTTTCAACAAGTATTAAACGATGCTAAAAAAGAGGATATTTTTATTATTGGCGGCAAGGAATTATTTGATATTTTTTTAGCTTATGCAGATGTTTTAATTGTTTCTGTATTAAAAGATTATTATGATTGTGATCTATACATGAAAGTTGATTATAATAATTTTAATTTAGATAAACAAGATGTTTATGATAATTTTATTGTTAATTATTATTCAAATAAGAAGGAAAAATAA", "aa": "MGKNTFDSLDQKPLNNRTNIIFSSIMQTPEDESYFVTNDFQQVLNDAKKEDIFIIGGKELFDIFLAYADVLIVSVLKDYYDCDLYMKVDYNNFNLDKQDVYDNFIVNYYSNKKEK", "aa_hexdigest": "5d0f9ee231a0a6c4e30426b0cf5e5d05", "start_type": "ATG", "rbs_motif": null, "genes": [ "folA" ], "truncated": "3-prime", "pseudogene": { "start": 27923, "stop": 28393, "observations": { "mutation": [ 28330, 28378 ], "stop": [ 28330, 28378 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PR30", "query_cov": 1.0, "subject_cov": 0.7371794871794872, "identity": 1.0, "score": 229.0, "evalue": 1.65e-75, "gene_start": 1, "gene_end": 115, "reference_start": 42, "reference_end": 156, "reference_sequence": "MLKLIWCQTLNGGISKNNKLPWYIKEELDHFYKTTKNHKIIMGKNTFDSLDQKPLNNRTNIIFSSIMQTPEDESYFVTNDFQQVLNDAKKEDIFIIGGKELFDIFLAYADVLIVSVLKDYYDCDLYMKVDYNNFNLDKQDVYDNFIVNYYSNKKEK" }, "paralog": false, "identity": 0.9871794871794872, "score": 314.0, "evalue": 1.07e-111, "description": "Internal stop codon at 28330, 28378. Nonsense mutation around 28330, 28378.", "psc": { "uniref90_id": "UniRef90_Q9PR30", "gene": "folA", "product": "Dihydrofolate reductase", "ec_ids": [ "1.5.1.3" ], "uniref50_id": "UniRef50_Q9PR30", "cog_id": "COG0262", "cog_category": "H", "kegg_orthology_id": "K00287", "go_ids": [ "GO:0005829", "GO:0004146", "GO:0050661", "GO:0046452", "GO:0046655", "GO:0006730", "GO:0046654" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PR30", "EC:1.5.1.3", "UniRef:UniRef50_Q9PR30", "COG:COG0262", "COG:H", "KEGG:K00287", "GO:0005829", "GO:0004146", "GO:0050661", "GO:0046452", "GO:0046655", "GO:0006730", "GO:0046654" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PR30", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR30" ], "product": "Dihydrofolate reductase" } }, "id": "NKIECMJPJD_83", "locus": "GCA000828735_00044" }, { "type": "rRNA", "sequence": "contig_1", "start": 28536, "stop": 28639, "strand": "-", "gene": "rrf", "product": "5S ribosomal RNA", "coverage": 0.8739495798319328, "score": 82.2, "evalue": 1.1e-13, "db_xrefs": [ "GO:0005840", "GO:0003735", "RFAM:RF00001", "KEGG:K01985", "SO:0000652" ], "nt": "TGTAGTGATTATATCAGAGTGGAAATACCTGTTCCCATCCCGAACACAGAAGTCAAGCACTCTAGAGCCGAAAATAGCGCAAGTAAAATAGGTCATCGCTACGC", "id": "NKIECMJPJD_34", "locus": "GCA000828735_00045" }, { "type": "rRNA", "sequence": "contig_1", "start": 28704, "stop": 31614, "strand": "-", "gene": "rrl", "product": "23S ribosomal RNA", "coverage": 0.9952136752136752, "score": 2802.8, "evalue": 3.8e-06, "db_xrefs": [ "GO:0005840", "GO:0003735", "RFAM:RF02541", "KEGG:K01980", "SO:0001001" ], "nt": "TAATAAGTTACTAAGAGCTTATGGTGAATGCCTTGGGACAAACAGGCGATGAAGGACGTGCTAATCTGCGATAAGCAACGGGTAGCTGATAAGAGGCTTTAATCCGTTGATCTCCGAATGAGGAAACTCAATCTAATGAGAATTAGATTACTGTATAAATGAATTCATAGTTTAACAGAGCGATACCTGGTGAAGTGAAACATCTCAGTAACCAGAGGAAAAGAAAACGAAGTGATTCCCTGTGTAGCGGCGAGCGAAAGGGGAGTAGGCCAAACCGAATTTCGATTCGGGGTTGTAGGACTACAATATGGAATAGAGAATGATAGTAGAATTGGTTGGGAAGCCAAATCATAGAGGGTGATAATCCCGTATACGAAATCATTCTTTTACCTAGTAGGATCCTGAGTAGGGCGGGACACGTGAAATCCTGTCTGAATCCACCCAGACCATTGGGTAAGCCTAAATACTAGTTTGTCACCGATAGAGCATAGTACCGTGAGGGAACGGTGAAAAGAACCCAGAGATGGGAGTGAAATAGAACCTGAAACCATAAGCTTACAAGGTGTTAGAGCACATTAATGTGTGATAGCGTGCCTTTTGAAGTATGAGCCAGCGAGTTATTATAGCATGCGAGGTTAAATCGTAGAGATGGAGCCGTAGGGAAACCGAGTCTTAATAGGGCGTTTTAGTATGTTATAATAGACGCGAAACGGGGTGATCTATCCATGGGCAGGTTGAAGGTGAAGTAACATTCACTGGAGGACCGAACCCACTTTCGTTGAAACGACAGGGGATGACCTGTGGATAGTGGTGAAATTCCAATCGAACTCCGTGATAGCTCGTTCTCGTCGAAATATTTTTAGGAATAGCGTTAGATTATTGGGAATATGGGGGTAAAGCACTGAATCTATGATGGCGCCACCTCGGTGTACTGAATAGAATCAAACTCTGAATACCATATTACCTATTCTAGCAGTCAGACAGTGGGGGATAAGCTTCATTGTCGCGAGGGAAACAGCCCAGATCATTAACTAAGGTCCCTAATATATGCTAAGTGGAAAACGATGTTGAGTTTCATAAACAGCAAGGATGTTGGCTTAGAAGCAGCCACCGTTTAAAGAGTGCGTAACAGCTCACTTGTCGAGAGACTCTGCGCGGAAGATGTAACGGGGCTAAGCATATAACCGAAGTTATGGGTTACATATTATATATGTAGCGGTAGACGAGTGTTGTATATGGGGCGAAGGTAGACTGTGAAGACTACTGGACTTTATACAAGTAAGAATGCTGGCGTGAGTAACGAATGAGAGTGAGAATCTCTCAAACCGATTGACTAAGGGTTCCTGGGCAAGGGTCGTCCTCCCAGGGTAAGTCGGATCCTAAGGCGAGGCTGAAAAGCGTAGTCGATGGAAAACAGGTTAATATTCCTGTACCTACAAATTGTGTGATGGAGTGACGGAGAAGGTTATTATGTGCCGGTTATTGGATTCCGGTTTAAATAACAAGGTTAGTAAGTTGGCAAATCCGCTTACTATTAAGACCAAGTTATGAATACGAGCGACCCCTTCGGGCAGTAGCGAAGACATATACATCATGCTTCCAAGAAAAGCTTCTAGCGTTAAACAATTAGTAGTCCGTACCGAGAACGAACACACGTGGTCAAGGAGAATATCCTAAGGTTAGCGAGTTAACTACAGTTAAGGAACTCTGCAAATTAACCCCGTACGTTAGCAATAAGGGGTGCTCGCTGTAAAAGGTGAGCCGCAGTGAATAGCGAGGGGGGACTGTTTAACAAAAACACAGCTCTATGCTAAGTCGTAAGACGATGTATATGGGGTGACACCTGCCCAATGCTGTAAGGTTAAAGAAGAATGTTAGCGCAAGCGAAGCTTTTAACTGAAGCCCCAGTGAATGGCGGCCGTAACTATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAATTCCGTCCCGCTTGAATGGTGTAACCATCTCTTGACTGTCTCAACTGTAGACTCGGTGAAATCCTGGTGAGGGTGAAGACGCCCTCTTGGCGTGATTGGACGGAAAGACCCCATGAAGCTTTACTGTAGCTTAATATTGGGAAATTTTATTACTTGTAGAGCATAGGTAGGAGACTGTGAAGTATACTCGCTAGGGTATATGGAGTCAACGTTGGAATACTACCCTTGTGATAAGATTTCTCTAACCTGCAGCCATGAATCTGGCTGGGGGACAGTGTTAGGTGGGCAGTTTGACTGGGGCGGTCGCCTCCCAAAAGGTAACGGAGGCGCGCAACGGTACCCTCAGCACGGTTGGAAATCGTGTATAGAGTGTAATGGTATAAGGGTGCTTGACTGTGAGACTTACAAGTCGAACAGGTAGGAAACTAGGTCATAGTGATCCGGTGGCTCAGAATGGAATGGCCATCGCTCAACGGATAAAAGCTACTCTGGGGATAACAGGCTGATAGTGCCCAAGAGTTCATATCGACGGCACTGTTTGGCACCTCGATGTCGACTCATCTCATCCTGGAGCTGAAGCAGGTTCCAAGGGTTCGGCTGTTCGCCGATTAAAGAGATACGTGAGTTGGGTTCAAACCGTCGTGAGACAGGTTGGTCCCTATCTGTCATGCCCGTAGGAAGATTGAGAAGAGCTGTTCCTAGTACGAGAGGACCGGAATGGACACACCTCTTGTGATCCTGTTGTCGCGCCAGCGGCACTGCAGGGTAGCAACGTGTGGAATAGAGAAACGCTGAAAGCATCTAAGTGTGAAACTAACTTCAAGATTAATCTTCCCTTTCTTAAATGAAGTAAGAATCGTTAAAGACTATAACGTTGATAGGTCGGATGTGTACGCACGGTGACGTGTTTAGCTAACCGATACTAATAATTCGAGGACTTATATAA", "id": "NKIECMJPJD_36", "locus": "GCA000828735_00046" }, { "type": "rRNA", "sequence": "contig_1", "start": 31907, "stop": 33423, "strand": "-", "gene": "rrs", "product": "16S ribosomal RNA", "coverage": 0.9895629484670581, "score": 1449.2, "evalue": 1.6e-44, "db_xrefs": [ "GO:0005840", "GO:0003735", "RFAM:RF00177", "KEGG:K01977", "SO:0001000" ], "nt": "ATTTTAAAGAGTTTGATCCTGGCTCAGGATTAACGCTGGCGGCATGCCTAATACATGCAAATCGAACGAAGCCTTTTAGGCTTAGTGGTGAACGGGTGAGTAACACGTATCCAATCTACCCTTAAGTTGGGGATAACTAGTCGAAAGATTAGCTAATACCGAATAATAACATCAATATCGCATGAGAAGATGTAGAAAGTCGCTCTTTGTGGCGACGCTTTTGGATGAGGGTGCGACGTATCAGATAGTTGGTGAGGTAATGGCTCACCAAGTCAATGACGCGTAGCTGTACTGAGAGGTAGAACAGCCACAATGGGACTGAGACACGGCCCATACTCCTACGGGAGGCAGCAGTAGGGAATTTTTCACAATGGGCGCAAGCCTTATGAAGCAATGCCGCGTGAACGATGAAGGTCTTATAGATTGTAAAGTTCTTTTATATGGGAAGAAACGCTAAGATAGGAAATGATTTTAGTTTGACTGTACCATTTGAATAAGTATCGGCTAACTATGTGCCAGCAGCCGCGGTAATACATAGGATGCAAGCGTTATCCGGATTTACTGGGCGTAAAACGAGCGCAGGCGGGTTTGTAAGTTTGGTATTAAATCTAGATGCTTAACGTCTAGCTGTATCAAAAACTGTAAACCTAGAGTGTAGTAGGGAGTTGGGGAACTCCATGTGGAGCGGTAAAATGCGTAGATATATGGAAGAACACCGGTGGCGAAGGCGCCAACTTGGACTATCACTGACGCTTAGGCTCGAAAGTGTGGGGAGCAAATAGGATTAGATACCCTAGTAGTCCACACCGTAAACGATCATCATTAAATGTCGGCCCGAATGGGTCGGTGTTGTAGCTAACGCATTAAATGATGTGCCTGGGTAGTACATTCGCAAGAATGAAACTCAAACGGAATTGACGGGGACCCGCACAAGTGGTGGAGCATGTTGCTTAATTTGACAATACACGTAGAACCTTACCTAGGTTTGACATCTATTGCGATGCTATAGAAATATAGTTGAGGTTAACAATATGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTTTCGTTAGTTACTTTTCTAGCGATACTGCTACCGCAAGGTAGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATATCTAGGGCTGCAAACGTGCTACAATGGCTAATACAAACTGCTGCAAAATCGTAAGATGAAGCGAAACAGAAAAAGTTAGTCTCAGTTCGGATAGAGGGCTGCAATTCGTCCTCTTGAAGTTGGAATCACTAGTAATCGCGAATCAGACATGTCGCGGTGAATACGTTCTCGGGTCTTGTACACACCGCCCGTCAAACTATGGGAGCTGGTAATATCTAAAACCGCAAAGCTAACCTTTTGGAGGCATGCGTCTAGGGTAGGATCGGTGACTGGAGTTAAGTCGTAACAAGGTATCCCTACGAGAACGTGGGGATGGATCACCTCCTTT", "id": "NKIECMJPJD_33", "locus": "GCA000828735_00047" }, { "type": "cds", "sequence": "contig_1", "start": 33691, "stop": 34626, "strand": "-", "frame": 3, "gene": "rluA", "product": "Pseudouridine synthase (Uracil hydrolase)", "db_xrefs": [ "COG:COG0564", "COG:J", "SO:0001217", "UniRef:UniRef50_Q9PR31", "UniRef:UniRef90_Q9PR31" ], "nt": "ATGAATTTAAAAGAAATTAAAATTAAAATTAACGATGCCAATCAACGTTTAGATCGTTTTTTATTAAAAAATTTTCCTAATTTATCAAGAATTGCTGTTTATAAAATAATTAGAACAAAAGATGTTAAAGTTAATCATAAGAAAGTTGATCATAATTATTTATTAAAAGAAAATGATGTTGTTAGTATTTATGCTAAGGAGAATTTTTTAGAAAAAAAAGTTGACTTATCTTTTGCTAATGCTAAAGATGAATTAGATATTATATATGAAGATAAAAATATTTTAGTTGTTCACAAACCGATTGGTTTAATTGTTCATGAAGATAATAGATATAAAAATGACACTTTACAAAATAGAATTAAGAAATATTTAGTTAAAAAGGATGAATATCATCCTTTTGATGAAAGTGCTTTTGTTCCTAGCTTATGTCATCGTTTAGATTTAAATACAAGTGGATTAATTGTTGCAGCTAAAACTGCAAATGCTTTAAGAGTAATTACTGAAATGTTTAAAAATAATGATGTGATTCGAATTTATAAATGTTTAACATACAATCAATTACCTAAAAATAATGATGTTATTTATAATTTCATGTATAAAGCTGATGATAACACAATGATTGTACAAAATTTTAAAACAAAATTTAATAAAACAGCAATTACCAAATATACATATATAGATAAGTATAAAAAATATTTTATTTATGACATTGAACTATTAACTGGAAGAACCCACCAAATACGAGCAGTTTTAAATTTTTTGCATGCACCAATTGTTGGTGAACAAAAATACATCACTAAAGATATTGATAAGAATCAAAATTATATGTATCAATGTTTAGTATCATATAAGATCAAATTTATTAATTTAAAGAAATATCAATGTGAAGAATTAAATTATTTAGAGAATAAAGAATTTAGAATAAAAAATATTTGA", "aa": "MNLKEIKIKINDANQRLDRFLLKNFPNLSRIAVYKIIRTKDVKVNHKKVDHNYLLKENDVVSIYAKENFLEKKVDLSFANAKDELDIIYEDKNILVVHKPIGLIVHEDNRYKNDTLQNRIKKYLVKKDEYHPFDESAFVPSLCHRLDLNTSGLIVAAKTANALRVITEMFKNNDVIRIYKCLTYNQLPKNNDVIYNFMYKADDNTMIVQNFKTKFNKTAITKYTYIDKYKKYFIYDIELLTGRTHQIRAVLNFLHAPIVGEQKYITKDIDKNQNYMYQCLVSYKIKFINLKKYQCEELNYLENKEFRIKNI", "aa_hexdigest": "d8a0318b1adbe586f34fce95c6092cb9", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "psc": { "uniref90_id": "UniRef90_Q9PR31", "query_cov": 1.0, "subject_cov": 0.9873015873015873, "identity": 1.0, "score": 619.0, "evalue": 7.74e-224, "valid": true, "gene": "RluA", "product": "Pseudouridine synthase (Uracil hydrolase)", "uniref50_id": "UniRef50_Q9PR31", "cog_id": "COG0564", "cog_category": "J" }, "pscc": { "uniref50_id": "UniRef50_Q9PR31", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR31" ], "product": "Pseudouridine synthase (Uracil hydrolase)" }, "genes": [ "rluA" ], "id": "NKIECMJPJD_84", "locus": "GCA000828735_00048" }, { "type": "cds", "sequence": "contig_1", "start": 34650, "stop": 35777, "strand": "-", "frame": 1, "gene": "dnaQ", "product": "putative protein UU112", "db_xrefs": [ "COG:COG0847", "COG:L", "GO:0003676", "GO:0004527", "GO:0006259", "RefSeq:WP_006689147.1", "SO:0001217", "UniParc:UPI00001392AB", "UniRef:UniRef100_Q9PR32", "UniRef:UniRef50_Q9PR32", "UniRef:UniRef90_Q9PR32" ], "nt": "ATGAAAATATGTGATAATATTAGTGAAATAAATGATGATCAGGATTTAGTCTTTATTGATATTGAAGCAACAGATAGTAAGGGCGAACAACGAATTATTCAGTTTAGCGGATACCGTTTAAATATTAAAAAAAATAAAATTTTTAATTTTAATAAAAAATTCAATCCTGAACAAGAAATTAATTCACGTATTAAAACCTTGCTGAACTTTAATAAAAATTTTAATAATATTGAACAAATGCCAAAATTAGATAAGCAAGCAGCAAGAGAAATTTATTGTTTTGTTAAAAATGCGATTGTCATTACTTTCACAGATTTTGATATTAAAAAAATGCACGAGCTTTTTACATATTATAATTTCGATTTTGAAAAAATCGTTTATTTTGATGTTTATAAGTTTTTTGAAAAAAAATTACAAACTAAATCAGTTCCTAGTTTATTTTCATTAGGAATTCTAAGTGGAATTAAAATTAATTTTTTCAAATTACATAATGCGCTATATGATGCTTTTATTTTAAAAGAAATTTTTATGTGTATTCGTCATAAAACGAACGAAGAATTATATGAAATGTATCGTTATTATGAATTTTTGCCAAAAATTATTTCTTCTTCATATTTTGTTACTAATGAACAAGAAAAAAACAGGGGAATTATTAAAAAAGAAATTAAGTATGTAATGTATATTAAAGAATTTGATTTCTCAAATAAATTTAATTTAGACTTTGTTGTTTATAAAAAAGATCATCATTTTTATTCAAAACCAATTTATGATAGTAGTTTAGAATTGCAAACAATTTCTTCACTAACTTCCAAATCTAATTTTATGAAAGTTAAATTAGCAAACATCTTTTTTAATTATTTAAGTAAAAGTGCTGTTTTTAGTATAAAAAAACTATCAATAAAACAAAGTGAAAAATTCTTAAAATTTTATAAAACACAGACTAATAAAAGAAAGGTTATTAAAGTTTTAAATTTAAATCTTAAAAAAGAAATTAAACCTGAGAATTTTGTCATAAAAGCTCAAATAATTTGTGAAATACTTTTTAAAAACCCAATAATTCATCATTTTGTACATGAATATTTAAAAATTTTTCAAAATATATTTTCTCAAGAAGAAAAAGATAATTAA", "aa": "MKICDNISEINDDQDLVFIDIEATDSKGEQRIIQFSGYRLNIKKNKIFNFNKKFNPEQEINSRIKTLLNFNKNFNNIEQMPKLDKQAAREIYCFVKNAIVITFTDFDIKKMHELFTYYNFDFEKIVYFDVYKFFEKKLQTKSVPSLFSLGILSGIKINFFKLHNALYDAFILKEIFMCIRHKTNEELYEMYRYYEFLPKIISSSYFVTNEQEKNRGIIKKEIKYVMYIKEFDFSNKFNLDFVVYKKDHHFYSKPIYDSSLELQTISSLTSKSNFMKVKLANIFFNYLSKSAVFSIKKLSIKQSEKFLKFYKTQTNKRKVIKVLNLNLKKEIKPENFVIKAQIICEILFKNPIIHHFVHEYLKIFQNIFSQEEKDN", "aa_hexdigest": "c6fe05234d3446a20aa043ecf2091666", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI00001392AB", "ncbi_nrp_id": "WP_006689147.1", "uniref100_id": "UniRef100_Q9PR32", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001392AB", "RefSeq:WP_006689147.1", "UniRef:UniRef100_Q9PR32" ] }, "ips": { "uniref100_id": "UniRef100_Q9PR32", "uniref90_id": "UniRef90_Q9PR32" }, "psc": { "uniref90_id": "UniRef90_Q9PR32", "gene": "DnaQ", "product": "Uncharacterized protein UU112", "uniref50_id": "UniRef50_Q9PR32", "cog_id": "COG0847", "cog_category": "L", "go_ids": [ "GO:0004527", "GO:0003676", "GO:0006259" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PR32", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR32" ], "product": "Uncharacterized protein UU112" }, "genes": [ "dnaQ" ], "id": "NKIECMJPJD_85", "locus": "GCA000828735_00049" }, { "type": "cds", "sequence": "contig_1", "start": 36188, "stop": 36442, "strand": "-", "frame": 2, "gene": null, "product": "putative protein UU111", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR33", "UniRef:UniRef90_Q9PR33" ], "nt": "ATGAATTATAAATATAGTGCTGAAGAAAAACAAATTATTTATAACTATATTTTAAGAGAATATGGGCAAGTGGACCACATTATATTTTTATCAAATGAACATATACGTGTTCCGATTGAATATGATATTTTAGTTATTAAAAAACAAAACTTACAAATATTAATGACTTTTGGACTAGGTGCTTTTAAATCACATAATCACGAAGAAAATACGCAAGAGCGTGCTGAGATTTTTTTAGAATTACCTGTTGATTGA", "aa": "MNYKYSAEEKQIIYNYILREYGQVDHIIFLSNEHIRVPIEYDILVIKKQNLQILMTFGLGAFKSHNHEENTQERAEIFLELPVD", "aa_hexdigest": "eb03bafc78c4c5679b6d1b81808f308c", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 35885, "stop": 36442, "observations": { "mutation": [ 36028, 36100, 36163, 36190 ], "stop": [ 36028, 36100, 36163, 36190 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PR33", "query_cov": 1.0, "subject_cov": 0.42, "identity": 1.0, "score": 171.0, "evalue": 1.82e-52, "gene_start": 1, "gene_end": 84, "reference_start": 1, "reference_end": 84, "reference_sequence": "MNYKYSAEEKQIIYNYILREYGQVDHIIFLSNEHIRVPIEYDILVIKKQNLQILMTFGLGAFKSHNHEENTQERAEIFLELPVDWDFNKHENMWPVHFLINIVKYSYSNHLTLKWLQTFINPSYFNKSNKIAGFLDLSWYGENSLECKINNDFFVSFYQILIIDDEELFYAKTNGIRALSKFFDDGKSRIVDLNRKSFVK" }, "paralog": false, "identity": 0.9783783783783784, "score": 363.0, "evalue": 7.54e-131, "description": "Internal stop codon at 36028, 36100, 36163, 36190. Nonsense mutation around 36028, 36100, 36163, 36190.", "psc": { "uniref90_id": "UniRef90_Q9PR33", "product": "Uncharacterized protein UU111", "uniref50_id": "UniRef50_Q9PR33", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PR33", "UniRef:UniRef50_Q9PR33" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PR33", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR33" ], "product": "Uncharacterized protein UU111" } }, "id": "NKIECMJPJD_86", "locus": "GCA000828735_00050" }, { "type": "cds", "sequence": "contig_1", "start": 36540, "stop": 37841, "strand": "-", "frame": 1, "gene": "potD", "product": "Lipoprotein", "db_xrefs": [ "COG:COG0687", "COG:E", "SO:0001217", "UniRef:UniRef50_A0AAC9T0J9", "UniRef:UniRef90_A0AAC9T0J9" ], "nt": "ATGCAATTAGAAGGGGATAATAAAAATAACATTATTAAAATTATTAATCATGAAATTCTAGGAATTAATAATAGTATTAGTCAAATTTTACATATTTTAAAAAATCCGAATATAAAAGAAGATATTAAAGAAGAAAATATTAAAAAATTAAAGCGTTTGCAAAAACAATTAGAAGATAGACAAAAAGAATTAAAAACGAATGTGTTCTTACCAGATAGTAGTAGTGTATCTTATATTGAAGATAAATTAAACAATATTAGTAATATGTTTAAAAATCTACATCCTAACTCTATGTATTTAAAAGCAAATTCAAACGATGTATTAAATGATTTAGCAATGGCTAATGTTGCTGGAGCAATTGCTTATAGTGGCGATATTGCTTTTGCTGCTAATGGGGGCGAGTATACAACTGAAGCTGATTCAAATTATGCAAACATGAAGCCAACATCAGATAATTTTCATGTTGTACGACCTAAAGATACGATGAGTGTTTTAGATGGTTTTACAATTAGTAACTCAATTAATAAACAAAACGAACAAGCAGCCTATGAATATTTAAATGAATTATGTTTTGCTGGTCTTAATGAAAAAAACACAGATGGAGAATCTAAAATTTTAGACTATGGTTTGAAAATTCATCGTAATAAAACACCAAAAGAAATTGCTGATTACGAAAATAGTTTAGATGATCAAAATGAAGTTGGTGACGTTTTAGAAGATAGTGGATATTTGTATACGCCAATGCGTAATTTTGATTATGTTCAATATTCGCCAACACTAAATACAATTGCTGATTATGTTTTAGATGAAAACAATGGATTTTATGGAAAAACTGGTTTTGAAAGTGATTTATTAAGACAAAAAATGGTTGAAATCTTTGAAATTGATACAAAATATAAAACAAAAAAAGAGCGCCAACGTAAACATAAATATATTTCATTATTAAATGAAATTATTAATCACATTTATAATACTTATCAAAAAGATAAAAAATTTATTTTTAATCCTACGTTAATTAATGATATATACGAACAAAAACCGATTAAAAATTTAATATATGAGTGTTTAAATAATTTAAATATTAAAATTGAATCACATGTTTTTAATAATTATCTTTTATTAAAATTAGAAGCTATTTTTAATAACATCAATGAAGAGGTTGAAGAACACGCTGCTTTAAACGCGACATTAAGAAAAATTTTTAATGTTCGTTTAAATATTAATACTTTAGAATTTCCCACAAATGATTTATCAATTTCTAATTTAAAATTAGCATACCTTGCCTTCCGTGAAAAGATTTAA", "aa": "MQLEGDNKNNIIKIINHEILGINNSISQILHILKNPNIKEDIKEENIKKLKRLQKQLEDRQKELKTNVFLPDSSSVSYIEDKLNNISNMFKNLHPNSMYLKANSNDVLNDLAMANVAGAIAYSGDIAFAANGGEYTTEADSNYANMKPTSDNFHVVRPKDTMSVLDGFTISNSINKQNEQAAYEYLNELCFAGLNEKNTDGESKILDYGLKIHRNKTPKEIADYENSLDDQNEVGDVLEDSGYLYTPMRNFDYVQYSPTLNTIADYVLDENNGFYGKTGFESDLLRQKMVEIFEIDTKYKTKKERQRKHKYISLLNEIINHIYNTYQKDKKFIFNPTLINDIYEQKPIKNLIYECLNNLNIKIESHVFNNYLLLKLEAIFNNINEEVEEHAALNATLRKIFNVRLNINTLEFPTNDLSISNLKLAYLAFREKI", "aa_hexdigest": "232ad2579a92284405bd3c4aa804a9d5", "start_type": "ATG", "rbs_motif": null, "genes": [ "potD" ], "truncated": "3-prime", "pseudogene": { "start": 36540, "stop": 38141, "observations": { "mutation": [ 38021 ], "stop": [ 38021 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAC9T0J9", "query_cov": 1.0, "subject_cov": 0.6386430678466076, "identity": 0.995, "score": 791.0, "evalue": 2.68e-284, "gene_start": 1, "gene_end": 433, "reference_start": 246, "reference_end": 678, "reference_sequence": "MKINKKLLKIFAGITSLLVFIPIITACSQKSKLVVGNFDSYMDPDVANEQAKRFRKNNLSYSFYDNNEILSSLIKAKISDVQVASAYEVAKLAKNGLIKKINWAKFNLKDENNQTITSIEGLKKIFTKQVWAISNAYSSYLGDLDKDGKNDQLLEYMIPYFYQDLIFAYRGKKIPNLDDNKKDVYWSDVFKELTRNDGTPNRFLYEQKANLGFGVDKRTPITIAKNKTKIIAIDDARTIYDIAKIMQLEGDNKNNIIKIINHEILGINNSISQILHILKNPNIKEDIKEENIKKLKRLQKQLEDKQKELKTNVFLPDSSSVSYIEDKLNNISNMFKNLHPNSMYLKANSNDVLNDLAMANVAGAIAYSGDIAFAANGGEYTTEPDSNYANMKPTSDNFHVVRPKDTMSVLDGFTISNSINKQNEQAAYEYLNELCFAGLNEKNTDGESKILDYGLKIHRNKTPKEIADYENSLDDQNEVGDVLEDSGYLYTPMRNFDYVQYSPTLNTIADYVLDENNGFYGKTGFESDLLRQKMVEIFEIDTKYKTKKERQRKHKYISLLNEIINHIYNTYQKDKKFIFNPTLINDIYEQKPIKNLIYECLNNLNIKIESHVFNNYLLLKLEAIFNNINEEVEEHAALNATLRKIFNVRLNINTLEFPTNDLSISNLKLAYLAFREKI" }, "paralog": false, "identity": 0.9943714821763602, "score": 1014.0, "evalue": 0.0, "description": "Internal stop codon at 38021. Nonsense mutation around 38021.", "psc": { "uniref90_id": "UniRef90_A0AAC9T0J9", "gene": "PotD", "product": "Lipoprotein", "uniref50_id": "UniRef50_A0AAC9T0J9", "cog_id": "COG0687", "cog_category": "E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAC9T0J9", "UniRef:UniRef50_A0AAC9T0J9", "COG:COG0687", "COG:E" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAC9T0J9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T0J9" ], "product": "Lipoprotein" } }, "id": "NKIECMJPJD_87", "locus": "GCA000828735_00051" }, { "type": "cds", "sequence": "contig_1", "start": 38019, "stop": 38108, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTCCTTATTTTTACCAAGATTTAATTTTTGCTTATCGTGGTAAAAAAATTCCTAATTTGGATGATAATAAAAAAGATGTTTATTGA", "aa": "MIPYFYQDLIFAYRGKKIPNLDDNKKDVY", "aa_hexdigest": "62c019cc97f47f991068c25f1ccff54f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3569.0892000000013, "isoelectric_point": 8.03239002227783 }, "id": "NKIECMJPJD_88", "locus": "GCA000828735_00052" }, { "type": "cds", "sequence": "contig_1", "start": 38268, "stop": 38576, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAATTAATAAAAAATTATTAAAAATTTTTGCTGGAATTACTAGTTTGTTAGTTTTTATACCTATAATTACAGCTTGTAGTCAAAAATCAAAACTTGTAGTTGGTAATTTTGATTCTTATATGGATCCAGATGTTGCTAATGAGCAAGCAAAACGATTTCGTAAAAATAATTTATCATATAGTTTTTATGATAATAATGAAATTTTAAGTTCATTAATTAAAGCAAAAATTTCAGATGTACAAGTTGCTTCAGCATATGAAGTAGCTAAATTAGCAAAAAATGGATTAATTAAGAAAATTAATTGA", "aa": "MKINKKLLKIFAGITSLLVFIPIITACSQKSKLVVGNFDSYMDPDVANEQAKRFRKNNLSYSFYDNNEILSSLIKAKISDVQVASAYEVAKLAKNGLIKKIN", "aa_hexdigest": "e738aaf252cbdacf2338c5536f05e237", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11401.27839999999, "isoelectric_point": 9.715466117858885 }, "id": "NKIECMJPJD_89", "locus": "GCA000828735_00053" }, { "type": "cds", "sequence": "contig_1", "start": 38595, "stop": 38699, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTTTATTAACAATTTTAATTGCTGCATTAATTAGTGCTAATAAAATTATTAAGTTAAAATTAATTAATAAACAAACTAATAAACGCAAATTAAAAATTTGA", "aa": "MVLLTILIAALISANKIIKLKLINKQTNKRKLKI", "aa_hexdigest": "fcdcc9f3f82b7a1140ced7bbdcd30057", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3858.8963000000012, "isoelectric_point": 11.471713829040528 }, "id": "NKIECMJPJD_90", "locus": "GCA000828735_00054" }, { "type": "cds", "sequence": "contig_1", "start": 38721, "stop": 39128, "strand": "-", "frame": 1, "gene": "potC", "product": "Spermidine/putrescine transport system permease", "db_xrefs": [ "COG:COG1177", "COG:E", "SO:0001217", "UniRef:UniRef50_Q9PR35", "UniRef:UniRef90_Q9PR35" ], "nt": "ATGACAGCATCATCACAAACAAACATAATGATTCCAGACGTAATTAGTGGTATTTCATTATCTTTATTATTTGCTGCTACTTTTATTCCAATTGGTTTTAGTTTTGGATTTGGTACGATTATATTAGCACACATTTCTTCTTGCACTCCTTATGCAATTATGATTATTTATCCTAGAATGTTAAAAATGAAAAAAAATTTAATTCTTGCTAGTTATGATTTAGGTTATACAAAAATTGCAACCTTTTTTAGAATTATTTTACCTTATTTATTACCTTCAATTATTTCAGCAACAATCATTGTTTTTGCTATGTCTTTTGATGATTTTATTATTACTAAATTGGTTGGAGGTAAAGTTAATACCATTGGAACAGAAATGTATTCGATGGCTAAAGGAATTAAAGCATGA", "aa": "MTASSQTNIMIPDVISGISLSLLFAATFIPIGFSFGFGTIILAHISSCTPYAIMIIYPRMLKMKKNLILASYDLGYTKIATFFRIILPYLLPSIISATIIVFAMSFDDFIITKLVGGKVNTIGTEMYSMAKGIKA", "aa_hexdigest": "47dc4da9900c1b63ec45d79783f866d0", "start_type": "ATG", "rbs_motif": null, "genes": [ "potC" ], "truncated": "both", "pseudogene": { "start": 38580, "stop": 39425, "observations": { "mutation": [ 38597, 38723, 39389, 39410, 39419 ], "stop": [ 38597, 38723, 39389, 39410, 39419 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PR35", "query_cov": 1.0, "subject_cov": 0.4753521126760563, "identity": 0.956, "score": 239.0, "evalue": 1.34e-77, "gene_start": 1, "gene_end": 135, "reference_start": 103, "reference_end": 237, "reference_sequence": "MNNINWLSWLRNSYIWIVLAIIYIPLIIIVLLSFTTPSIKGNITSAFDWNDGSNYLTLTTNQFTDALVNTIIVSIIAVPISTIIATMTCFGVWHAKAFYKKLMTASAQTNMMIPDVISGISLALLFAATFIPIGFSFGFSTIILAHISYCTPYAIMIIYPRMLKMKKNLILASYDLGYTKIATFFKIILPYLLPSIISATIIVFAMSFDDFIITKLVGGKVNTIGTEMYSMAKGIKAWAVCFGALMVLLTILIAALISANKIIKLKLINKQTNTRKLKIWNKQV" }, "paralog": false, "identity": 0.9252669039145908, "score": 499.0, "evalue": 2.85e-182, "description": "Internal stop codon at 38597, 38723, 39389, 39410, 39419. Nonsense mutation around 38597, 38723, 39389, 39410, 39419.", "psc": { "uniref90_id": "UniRef90_Q9PR35", "gene": "PotC", "product": "Spermidine/putrescine transport system permease", "uniref50_id": "UniRef50_Q9PR35", "cog_id": "COG1177", "cog_category": "E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PR35", "UniRef:UniRef50_Q9PR35", "COG:COG1177", "COG:E" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PR35", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR35" ], "product": "Spermidine/putrescine transport system permease" } }, "id": "NKIECMJPJD_91", "locus": "GCA000828735_00055" }, { "type": "cds", "sequence": "contig_1", "start": 39421, "stop": 39957, "strand": "-", "frame": 3, "gene": null, "product": "ABC transporter permease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR36", "UniRef:UniRef90_UPI0026F2788B" ], "nt": "ATGTTAGTAAAACTAATTGGTATTAAAACCTTTTTTGATGTAGTAAATGGAGAAATTAATAGTACACATGGGCACATTTTTACTATTTTAGGTTTAGTTTATATTAATTTGCCATTAATGATTTTGCCTATTTATACATCTTTGTCAACAATGCCTAAAAACCTAGTTAATGCTAGTTATGATTTAGGAAGAGGTTTTTTTTACACCTTCTTTCATATTGTAATTCCTTACACTAAAAATGCTTTAATTTCAGGAGTTTGGATTGTTTATTTAGCAGCATTTACATCAGTTGTGGTTTCAGGTTTTTTAAATAATGATAACGCTGGAGGACTAATTGGTGGAGAAATCTTTGCTCAAGGACAAGACGGTATTTCAAGTGGAGTTCAACTATCACGGTCATCAACAATTACTTTAGTAATTAGTTTAATTTTATTAGGGATTTATCTTATTATTGTTATTATTCCAAAAATAGTCCAATTAATTTATTACAAACGAAAAAATCTAAAAAAAGGAGTAAAGAATAATGAAGCAGATTAA", "aa": "MLVKLIGIKTFFDVVNGEINSTHGHIFTILGLVYINLPLMILPIYTSLSTMPKNLVNASYDLGRGFFYTFFHIVIPYTKNALISGVWIVYLAAFTSVVVSGFLNNDNAGGLIGGEIFAQGQDGISSGVQLSRSSTITLVISLILLGIYLIIVIIPKIVQLIYYKRKNLKKGVKNNEAD", "aa_hexdigest": "67eececb84801828bc3668369699cb98", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 39421, "stop": 40257, "observations": { "mutation": [ 40089 ], "stop": [ 40089 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0026F2788B", "query_cov": 1.0, "subject_cov": 0.5313432835820896, "identity": 0.978, "score": 330.0, "evalue": 8.23e-112, "gene_start": 1, "gene_end": 178, "reference_start": 158, "reference_end": 335, "reference_sequence": "MNKQFNFNFAPSKSSDINKKNETVFFLKEVKVIKKNNRKHYKQTLFDKLKMNKALILTIPYFLVTIFLLIIPLVSIIVKTFINNTDSTGVTTNIIDNWSVITPTIGMKIFNSLWIAAVATIFCLIIGYPFAYFLSIGKSKFLKAIAIALITSPIWMSMLVKLIGIKTFFDVVNGEINSTHGHIFTILGLVYINLPLMILPIYTSLSTMPKNLVNASYDLGRGFFYTFFHIVIPYTKNALISGVWIVYLAAFTSVVVSGFLNNDNAGGLIGGEIFAQGQDGISSGVQLSRSSTITLVISLILLGIYLIIVIIPRVVQLIYYKRKNLKKGAKNNETD" }, "paralog": false, "identity": 0.9820143884892086, "score": 533.0, "evalue": 2.76e-194, "description": "Internal stop codon at 40089. Nonsense mutation around 40089.", "psc": { "uniref90_id": "UniRef90_UPI0026F2788B", "product": "ABC transporter permease", "uniref50_id": "UniRef50_Q9PR36", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0026F2788B", "UniRef:UniRef50_Q9PR36" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PR36", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR36" ], "product": "Spermidine/putrescine transport system permease" } }, "id": "NKIECMJPJD_92", "locus": "GCA000828735_00056" }, { "type": "cds", "sequence": "contig_1", "start": 40087, "stop": 40428, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACAAGCAATTTAATTTCAATTTTGCACCATCTAAATTATCAGATATTAACAAAAAAAACGAAACAGTATTTTTTTTAAAAGAAGTTAAAGTAATTAAAAAAAACAATCGTAAGCATTATAAACAAACTCTTTTTGATAAATTAAAAATGAATAAGGCACTAATATTAACAATTCCTTATTTTTTAGTAACTATTTTTTTATTGATAATACCATTGGTTAGTATTATTGTTAAAACTTTTATTAACAATACTGATTCAACTGGTGTAACAACAAACATCATTGATAATTGGTCAGTAATTACGCCAACTATTGGAATGAAAATTTTTAATTCGTTATGA", "aa": "MNKQFNFNFAPSKLSDINKKNETVFFLKEVKVIKKNNRKHYKQTLFDKLKMNKALILTIPYFLVTIFLLIIPLVSIIVKTFINNTDSTGVTTNIIDNWSVITPTIGMKIFNSL", "aa_hexdigest": "45bb987cf420c488ab3301b519e21941", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13084.448399999983, "isoelectric_point": 10.05012149810791 }, "id": "NKIECMJPJD_93", "locus": "GCA000828735_00057" }, { "type": "cds", "sequence": "contig_1", "start": 40559, "stop": 40690, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATTAATGGTTTTAGCGAAAATGAAGCGTGTTATATTATGATTCGCCCAGAAGACTTTGATGTTGTTAAAAAAGACGAAGGATCAATTAATGCTAGAGTTGAATCAGTATTATATAAAGGGTTAATGTGA", "aa": "MINGFSENEACYIMIRPEDFDVVKKDEGSINARVESVLYKGLM", "aa_hexdigest": "9d9ed94bf0b52c142b4b809875e54a3d", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4912.5949, "isoelectric_point": 4.554986000061036 }, "id": "NKIECMJPJD_94", "locus": "GCA000828735_00058" }, { "type": "cds", "sequence": "contig_1", "start": 40784, "stop": 41059, "strand": "-", "frame": 2, "gene": null, "product": "ATP-binding cassette domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0034D25CD8" ], "nt": "ATGCAACAACGTGTTGCACTAGCACGTTCACTAGTAATACAGCCTGAAATTATTTTATTAGATGAACCATTAAGTGCACTAGATGCAAAAGTACGTAAACAATTACAAGATGAATTAAAAAAACTACATAAAAATTTAGGAATCACCTTTATTTTAGTAACTCATGATCAAGAAGAGGCTTTAAGTTTATCAGATAAAGTTGTGGTTATGTCAAATGGTCAAATCGAACAAGTCGGTAAACCAAGTGATATTTATGATTCACCAAATTCATTGTGA", "aa": "MQQRVALARSLVIQPEIILLDEPLSALDAKVRKQLQDELKKLHKNLGITFILVTHDQEEALSLSDKVVVMSNGQIEQVGKPSDIYDSPNSL", "aa_hexdigest": "2cc3bab9b23f163ffa50926492c45273", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0034D25CD8", "query_cov": 0.9230769230769231, "subject_cov": 0.9767441860465116, "identity": 0.631, "score": 111.0, "evalue": 2.11e-30, "valid": false, "product": "ATP-binding cassette domain-containing protein", "uniref50_id": "UniRef50_UPI0034D25CD8" }, "pscc": { "uniref50_id": "UniRef50_UPI0034D25CD8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0034D25CD8" ], "product": "ATP-binding cassette domain-containing protein" }, "genes": [], "id": "NKIECMJPJD_95", "locus": "GCA000828735_00059" }, { "type": "cds", "sequence": "contig_1", "start": 41228, "stop": 41350, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATTTAAACGATCCAATTGATAGAGAAATTTTTAATTTAAAAAAAGCATATAAAGAAAAAAGTGGATTAGATAAACGATATGATAAGATTACTTACAAATATAATGATCTTGATTATTGA", "aa": "MNLNDPIDREIFNLKKAYKEKSGLDKRYDKITYKYNDLDY", "aa_hexdigest": "075096705fb3af9cf191799c4873c087", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4932.520200000002, "isoelectric_point": 7.941489601135254 }, "id": "NKIECMJPJD_96", "locus": "GCA000828735_00060" }, { "type": "cds", "sequence": "contig_1", "start": 41366, "stop": 41971, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00005.33" ], "nt": "ATGGAAAAAACACTCCTACATCTTAGAGATATTACAAAAATCTATGATGATGGATTTGCTGCTGTAAACAAATTTGATTTAAAAATAAAAAAAGGTGAATTTGTCACTTTATTAGGACCAAGTGGTTGTGGTAAGACAACAATGTTAAAAATTATTGCTGGATTTGAACAACCTACTAATGGAAAAATTTTATATAATGGAATTGATATTAAGGATATGCCAATTCGACTAAGACCAACATCAACGGTGTTTCAAGACTATGCGCTATTCCCTAATATGACTGTTAAACAAAACATAAAATATGGTTTAAAACTAATGCGTAAACCAAAAGATAATGTTGATCAAAGTATTTATTTGCAAGCTGATAAAGTTTATAATTCAGCTTCTAAAAAAGCAAATGAAAAAATTAAAGAATTAAAAAAACAAAGACGCGGGTTATTGGCTGAAATCAAAAAAATGGATCTTAAATACCAAAAGAATAAGAACATTTTTGAAATAAAAGAAATGCGAAAAAACCAATATCTTGGCACACTTGATGAACTTTATCAAAAACAAGGTATTAATAAAAATGGAAAACCAGGTTTTTTAAATTATTTTAAAAGTTGA", "aa": "MEKTLLHLRDITKIYDDGFAAVNKFDLKIKKGEFVTLLGPSGCGKTTMLKIIAGFEQPTNGKILYNGIDIKDMPIRLRPTSTVFQDYALFPNMTVKQNIKYGLKLMRKPKDNVDQSIYLQADKVYNSASKKANEKIKELKKQRRGLLAEIKKMDLKYQKNKNIFEIKEMRKNQYLGTLDELYQKQGINKNGKPGFLNYFKS", "aa_hexdigest": "94af7db8b18ae48d5785397851ec9604", "start_type": "ATG", "rbs_motif": "GGAGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00005.33", "name": "ABC_tran", "length": 118, "aa_cov": 0.5771144278606966, "hmm_cov": 0.6694915254237288, "evalue": 4.4581867663898626e-23, "score": 77.9587631225586, "start": 23, "stop": 138 } ], "seq_stats": { "molecular_weight": 23240.02250000001, "isoelectric_point": 9.819195747375485 }, "id": "NKIECMJPJD_97", "locus": "GCA000828735_00061" }, { "type": "cds", "sequence": "contig_1", "start": 42611, "stop": 42931, "strand": "-", "frame": 2, "gene": null, "product": "aminoacyl--tRNA ligase-related protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI003418C7B1" ], "nt": "GTGCAATTAACAAATTTACATCGTAATGAAATTTTAAAAGCTAGTGATTTACCTTTATACTATACAGCTCTAACACCTTGTTTTCGTAGTGAAGCAGGATCTGCAGGACGCGATGTTAGAGGTGTAATTCGTCAACACCAATTCCATAAAGTAGAATTAGTAAAATTATGTAAACCAGAAGATAGTTTTAAAGAATTAGAATCAATGACAAGACAGGCTGAAAGTATTTTAGAAGCTTTAGAATTACCTTATAGAAGAATCGTTTTATGCACAGGCGATTTAGGTTTTAGTTCAGCTAAAACATATGATTTAGAAGTTTGA", "aa": "MQLTNLHRNEILKASDLPLYYTALTPCFRSEAGSAGRDVRGVIRQHQFHKVELVKLCKPEDSFKELESMTRQAESILEALELPYRRIVLCTGDLGFSSAKTYDLEV", "aa_hexdigest": "4770f0a50c1e985171ad26901e7db99e", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI003418C7B1", "query_cov": 0.9811320754716981, "subject_cov": 0.8125, "identity": 0.654, "score": 132.0, "evalue": 1.13e-37, "valid": false, "product": "aminoacyl--tRNA ligase-related protein", "ec_ids": [ "6.1.1.11" ], "uniref50_id": "UniRef50_UPI003418C7B1", "kegg_orthology_id": "K01875" }, "pscc": { "uniref50_id": "UniRef50_UPI003418C7B1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI003418C7B1" ], "product": "aminoacyl--tRNA ligase-related protein" }, "genes": [], "id": "NKIECMJPJD_98", "locus": "GCA000828735_00062" }, { "type": "cds", "sequence": "contig_1", "start": 43205, "stop": 43630, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02403.27" ], "nt": "ATGTTTGATATTAATTTAATACGAAAAGATATTGCAACTACTAAAGAAAAGATGTTAAATAAAAAGGTGCCAAGCAATTTGTTTGATCAAATTTTTGATTTAGATGTTTTAGTACGAAGTTTAATGCAACAAGAACAAAATCTTAATGCTAAGAAAAATCAATTATCAAAAGAAATTGGTATTTTAGCTAAAAACAAAGATCCTAAATTACAACAAACATTAGATTTAGTTAATAATATTAAAAACGAATTGCAAGATATTTCATTAACATTATCAAACAAACAAGATGAATTAAATAAATTATTATTAGTAATTCCTAATATGCCAGATGATTCAGTGCCCATTGGCAATGATGAAAATGACAACGTCGAAATTAAAAAAGTTTTTAAACCAAAAAAATTTGATTTTTTACCCCTTGCGCATTGA", "aa": "MFDINLIRKDIATTKEKMLNKKVPSNLFDQIFDLDVLVRSLMQQEQNLNAKKNQLSKEIGILAKNKDPKLQQTLDLVNNIKNELQDISLTLSNKQDELNKLLLVIPNMPDDSVPIGNDENDNVEIKKVFKPKKFDFLPLAH", "aa_hexdigest": "e44c934cab2ee6724594f692e4d2cc58", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02403.27", "name": "Seryl_tRNA_N", "length": 109, "aa_cov": 0.7730496453900709, "hmm_cov": 0.9908256880733946, "evalue": 1.4177536598333858e-25, "score": 85.21244812011719, "start": 1, "stop": 109 } ], "seq_stats": { "molecular_weight": 16223.643699999986, "isoelectric_point": 6.591072273254395 }, "id": "NKIECMJPJD_99", "locus": "GCA000828735_00063" }, { "type": "cds", "sequence": "contig_1", "start": 43741, "stop": 45081, "strand": "-", "frame": 3, "gene": "ftsH", "product": "ATP-dependent zinc metalloprotease FtsH", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449AS56" ], "nt": "ATGTTATATGGACCTCCAGGAACAGGAAAAACATTAATTGCTAAAGCTGTAGCTGGTGAAGCTAATGTTCCATTCTTTCAAACAACGGGTTCATCTTTTGAAGATACTTTTGTTGGAGTTGGTGCACGTCGTGTGAGAGAATTATTTGAAAAAGCACGTAAGAGTGCTCCCGCCATTATTTTTATTGATGAAATTGACTCAGTAGCTAAAAAACGTGGTAATTCATTAACTGCTGTACAAGACCAAACAATTAACCAATTATTATCAGAATTAGATGGTTTTGATACTTCAAGTGGTGTAATTGTTATGGCTGCGACAAATAGATTAGACACATTAGATGACGCAATTTTACGTCCTGGACGTTTTGACCGACAAATTAGTGTTAACTTACCAGATATTTTAGAACGTGAACAAATTTTAAGAATTCACTCACGTAATAAAAACTTATCTGCGAAAGTTAGTTTAGAAGATATTGCACGTCGTACTGCTGGATTTAGTGGTGCACAATTAGAAAATGTTTTAAATGAAGCCGCATTACTATCAGTTAGAGATAAAGCAACATCAATTCATATGAATCATTTAGATGAAGCGATTGATCGTGTTATTGCAGGACCTTCACGTCCTAATAAAGTTATTTCTGAACGTGAACGTGAACAAGTTTCATACCATGAAGCTGGACATGCATTAATTGGTTTATATTCACCAGGTGCTGATGTTGTTCAGAAAATTACTATTGTTGCTCGTGGTCGAGCTGCTGGTTATACACTACAAACTCCTGAAAGAAATGAAAATATTTTACAAAATAAAACTGAATTAATTAGTCGAGTGCGTACTGCATTAGGTGGTCGAGCTGCAGAAGAGTTAATTTATGGACCTAATGAAATTACAACAGGTGCAGCAAATGACTTTTATAAAATTACAAACATCGTAAGAGCAATGGTAGCTTCTTTTGGAATGACAGATGTGGGTTTAACGCAATATATTGCTACTGAAGGTGTTGATAACCCTTATCGAAATAGTTATTCAGAACAAACAGCATTAGCAATTGATATTGAAATTGAAAAAATTATTCAACGTGAATATAAAATTGTTAAAGAAATGATTAATGAACATCGTGAAGAATTGGAATTAATCGTTCAAACATTATTAGAATTAGAAACAATTTTAAAGCCACAAATTGATTATATTCACCAATACAAACAATTACCACCAGAGGTAATTGCTAATAAAAACAAACGTGAAGCTTCTCAAAAACAAGCAAATAGTAGTGTAGAAGAAGCAAAAGTTGTTGACGATGAAGAATCAATAAAAGATAAAGAAAAAGATCAAAAATCTAACTAA", "aa": "MLYGPPGTGKTLIAKAVAGEANVPFFQTTGSSFEDTFVGVGARRVRELFEKARKSAPAIIFIDEIDSVAKKRGNSLTAVQDQTINQLLSELDGFDTSSGVIVMAATNRLDTLDDAILRPGRFDRQISVNLPDILEREQILRIHSRNKNLSAKVSLEDIARRTAGFSGAQLENVLNEAALLSVRDKATSIHMNHLDEAIDRVIAGPSRPNKVISEREREQVSYHEAGHALIGLYSPGADVVQKITIVARGRAAGYTLQTPERNENILQNKTELISRVRTALGGRAAEELIYGPNEITTGAANDFYKITNIVRAMVASFGMTDVGLTQYIATEGVDNPYRNSYSEQTALAIDIEIEKIIQREYKIVKEMINEHREELELIVQTLLELETILKPQIDYIHQYKQLPPEVIANKNKREASQKQANSSVEEAKVVDDEESIKDKEKDQKSN", "aa_hexdigest": "a338375b60ffd4881e5d2916cbf1760c", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A449AS56", "query_cov": 0.9461883408071748, "subject_cov": 0.8559837728194726, "identity": 0.507, "score": 398.0, "evalue": 7.7e-132, "valid": false, "gene": "ftsH", "product": "ATP-dependent zinc metalloprotease FtsH", "uniref50_id": "UniRef50_A0A449AS56", "go_ids": [ "GO:0004222", "GO:0006508", "GO:0016020", "GO:0016887", "GO:0051301" ] }, "pscc": { "uniref50_id": "UniRef50_A0A449AS56", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449AS56" ], "product": "ATP-dependent zinc metalloprotease FtsH" }, "genes": [ "ftsH" ], "id": "NKIECMJPJD_100", "locus": "GCA000828735_00064" }, { "type": "cds", "sequence": "contig_1", "start": 45763, "stop": 45906, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATTTTTTAAAAAAAATAGTAAATTTATTTAGTTCAAAAATTGAATCAGAAGATAATAATGTTAAAAAAGACGACTTAACTCAACCACGTAAACAATCGCCAGAAGCGCGCAAACGAAGAAATCGAAGAATTATATTTTGA", "aa": "MYFLKKIVNLFSSKIESEDNNVKKDDLTQPRKQSPEARKRRNRRIIF", "aa_hexdigest": "55ecc66f877f2f0f85abb6f9112cd2e3", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5738.5858, "isoelectric_point": 10.432547950744627 }, "id": "NKIECMJPJD_101", "locus": "GCA000828735_00065" }, { "type": "cds", "sequence": "contig_1", "start": 45911, "stop": 46309, "strand": "-", "frame": 2, "gene": "hpt", "product": "hypoxanthine phosphoribosyltransferase", "db_xrefs": [ "EC:2.4.2.8", "GO:0006166", "KEGG:K00760", "SO:0001217", "UniRef:UniRef50_Q9PR40", "UniRef:UniRef90_Q9PR40" ], "nt": "ATGAAACTAGATTTTTTAGCAATTAGTAGTTTTAAGGGAGCAACTAGTGCTCAAACAGAACCAGAAATTATTACTGATCTTAAATTTGAAGTTAAAGATCGTGATCTAATTTTAGTAGAAGATATTGTTGATACAGGACGCACAATTAAAAAGGTTTATGATTTACTTAAAATTCGAGGCGCTCGTTCAATTAAATTAGTTACCTTGGTTGATAAAAAAGACGGCCGATTAGTAGATTTACAAGCCGATTTTGTATGTTGTGATATTCCATTAGTCTTTATTGTTGGTTTTGGTTTAGATTATAAAGAAATTATGCGTAATTTACCATATATTGGTGTTTTAAAAGAAGAAGTTTATCAAAATGATTTAAATAATAAAAACGAAGGGGATGGAGAGTAA", "aa": "MKLDFLAISSFKGATSAQTEPEIITDLKFEVKDRDLILVEDIVDTGRTIKKVYDLLKIRGARSIKLVTLVDKKDGRLVDLQADFVCCDIPLVFIVGFGLDYKEIMRNLPYIGVLKEEVYQNDLNNKNEGDGE", "aa_hexdigest": "f7ba9926841866765777b60726c5ce08", "start_type": "ATG", "rbs_motif": null, "genes": [ "hpt" ], "truncated": "3-prime", "pseudogene": { "start": 45911, "stop": 46531, "observations": { "mutation": [ 46411 ], "stop": [ 46411 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PR40", "query_cov": 1.0, "subject_cov": 0.6407766990291263, "identity": 1.0, "score": 261.0, "evalue": 4.02e-87, "gene_start": 1, "gene_end": 132, "reference_start": 75, "reference_end": 206, "reference_sequence": "MIKNQKTRNDGIKMKDIDPRIKEVLITEEQIDQKITEAANWINKEYEGKEPIIIGILKGCIPFIGKLLPKIKIDMKLDFLAISSFKGATSAQTEPEIITDLKFEVKDRDLILVEDIVDTGRTIKKVYDLLKIRGARSIKLVTLVDKKDGRLVDLQADFVCCDIPLVFIVGFGLDYKEIMRNLPYIGVLKEEVYQNDLNNKNEGDGE" }, "paralog": false, "identity": 0.9951456310679612, "score": 403.0, "evalue": 1.22e-145, "description": "Internal stop codon at 46411. Nonsense mutation around 46411.", "psc": { "uniref90_id": "UniRef90_Q9PR40", "gene": "hpt", "product": "hypoxanthine phosphoribosyltransferase", "ec_ids": [ "2.4.2.8" ], "uniref50_id": "UniRef50_Q9PR40", "kegg_orthology_id": "K00760", "go_ids": [ "GO:0006166" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PR40", "EC:2.4.2.8", "UniRef:UniRef50_Q9PR40", "KEGG:K00760", "GO:0006166" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PR40", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR40" ], "product": "Hypoxanthine phosphoribosyltransferase" } }, "id": "NKIECMJPJD_102", "locus": "GCA000828735_00066" }, { "type": "cds", "sequence": "contig_1", "start": 46409, "stop": 46531, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTAAAAATCAAAAAACAAGAAATGATGGTATAAAAATGAAAGATATTGATCCAAGAATAAAAGAGGTTTTGATCACTGAAGAACAAATTGATCAAAAAATCACTGAAGCAGCTAATTGA", "aa": "MIKNQKTRNDGIKMKDIDPRIKEVLITEEQIDQKITEAAN", "aa_hexdigest": "0b8f0c7b2728e348f4646757b14d4812", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4669.339300000001, "isoelectric_point": 6.125674629211424 }, "id": "NKIECMJPJD_103", "locus": "GCA000828735_00067" }, { "type": "cds", "sequence": "contig_1", "start": 46558, "stop": 47352, "strand": "-", "frame": 3, "gene": null, "product": "Iron ABC transporter substrate-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAP9D789", "UniRef:UniRef90_A0AAC9T332" ], "nt": "ATGAATGCCAAGAATTTGATTAATCCCAACAACCCTAATATTGCAAAAATTTTTAAAATTAAAGTTTTTAAAGAAAACGAAAAATTTAAACCAATAACCTTAACGTCAATTAATCAACAAGAAGCGGATGAATATGATCGACAAAGAAATCAAATTATTCATAAAAATAATTCAGAAATTGGGATATTAGATGATTCAAATAAAATTAAAGAGTTAGGAACTTTTTTATATTTTAAAAAACCTTTATCTGAAATTACAATCAACGATTTTGATCGTTATTTTAGAACTTATTTTATTTGTCCAGAGCAAGATTTTAATGATTTTAGTCGTCAATATAAAATTACTAAAATTGATAAACAAAAACAAATAATAGAACTAAGTATATTACATATTAATAATAAAACAAATGAAATTTATAGTTCACCAATTAATTTTAAGTTTTATTACAAAAAAATATATGATATTAACAAAAACACTGATGCACGATTAGATTTAAGTTTTGATGAATCTAAAAAAGAACAAAAACAAAAACTACAAGAGTTAGTTAATAAATTAAATTTTGATAAACTTACTTATAATGATTTAAGTGAATTAGCTGTTGTGATGTTTGAGGATGTGGATTTAGAAAACACTAAAATTTATGTTGAAATGATTAAAAATAAAGATCGTTCAATTACTTTAAAAGCTTTTGTTGATAAAGCTTTTAAAAATGGAAAATTAAAGGTAAGTAATAAACCAGATATTTTAATAAAAGAGTTTAAAATAAACTATTTTATGCCTAATTACTTTAAATAA", "aa": "MNAKNLINPNNPNIAKIFKIKVFKENEKFKPITLTSINQQEADEYDRQRNQIIHKNNSEIGILDDSNKIKELGTFLYFKKPLSEITINDFDRYFRTYFICPEQDFNDFSRQYKITKIDKQKQIIELSILHINNKTNEIYSSPINFKFYYKKIYDINKNTDARLDLSFDESKKEQKQKLQELVNKLNFDKLTYNDLSELAVVMFEDVDLENTKIYVEMIKNKDRSITLKAFVDKAFKNGKLKVSNKPDILIKEFKINYFMPNYFK", "aa_hexdigest": "8b81aa161daed45b79fd61fb5ef4c757", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 46558, "stop": 47652, "observations": { "mutation": [ 47541 ], "stop": [ 47541 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAC9T332", "query_cov": 1.0, "subject_cov": 0.47653429602888087, "identity": 0.992, "score": 508.0, "evalue": 1.95e-177, "gene_start": 1, "gene_end": 264, "reference_start": 291, "reference_end": 554, "reference_sequence": "MKHKTKKITLLIAGITLTSLCTIFAITACSKQKSQPNPKIKPNNVVEKVDQAFFNEFKTKIEQKRALPNIKITSTFKIPFERNENTLKSPLFKIGEKSVFDLKQNEIPITWVQDLKDYFLSQKTILTYYSLANLKLTGNDAKDAYFLEFIQYIQNMQQKGIILEDFYQQLWYLISEISDYDMQNGILRSVYRIANPKLAIKDFYLDGSLYGFKKEKSKYMNFTSLFQWPNAEEKATFDVVFKNKNSAQSYTKKIELIYSKEKKFILDSLINYSEFTPGEYELVSIKKHDDMNAKNLINPNNPNIAKIFKIKVFKENEKFKPITLTSINQQEADEYDRQRNQIIHKNNSEIGILDDSNKIKELGTFLYFKKPLSEITINDFDRYFRTYFICPEQDFNDFSRQYKITKIDKQKQIIELSILHINNKTNEIYSSPINFKFYYKKIYDINKNTDARLDLSFDESKKDQKQKLQELVNKLNFDKLTYNDLSELAVVMFEDVDLENTKIYVEMIKNKDRSITLKAFVDKAFKNGKLKISNKPDILIKEFKINYFMPNYFK" }, "paralog": false, "identity": 0.9917582417582418, "score": 724.0, "evalue": 7.87e-265, "description": "Internal stop codon at 47541. Nonsense mutation around 47541.", "psc": { "uniref90_id": "UniRef90_A0AAC9T332", "product": "Iron ABC transporter substrate-binding protein", "uniref50_id": "UniRef50_A0AAP9D789", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAC9T332", "UniRef:UniRef50_A0AAP9D789" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAP9D789", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAP9D789" ], "product": "Iron ABC transporter substrate-binding protein" } }, "id": "NKIECMJPJD_104", "locus": "GCA000828735_00068" }, { "type": "cds", "sequence": "contig_1", "start": 47539, "stop": 47679, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAAATGGTATTTTACGTTCAGTATATCGTATCGCTAATCCTAAACTTGCAATCAAGGATTTTTACCTTGATGGTTCGCTTTATGGATTTAAAAAAGAAAAAAGTAAATACATGAATTTTACAAGTTTGTTTCAATGA", "aa": "MQNGILRSVYRIANPKLAIKDFYLDGSLYGFKKEKSKYMNFTSLFQ", "aa_hexdigest": "5e1907f12d5596b45973ed11c12d3e72", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5425.2853000000005, "isoelectric_point": 9.82177448272705 }, "id": "NKIECMJPJD_105", "locus": "GCA000828735_00069" }, { "type": "cds", "sequence": "contig_1", "start": 47890, "stop": 48222, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAACATAAGACAAAAAAAATAACGCTTTTAATAGCAGGAATTACTTTAACTTCATTATGTACAATTTTTGCTATTACAGCTTGTAGTAAGCAAAAATCACAACCAAATCCAAAAATAAAACCCAATAAAATTATTGAGAAAATTGATCAAGCATTTTTTAATGAATTTAAAACAAAAATTGAACAAAAACGCACACTTCCAAATATTAAAATCACTTCAACCTTTAAAATTCCTTTTGAGAAAAACGAAAACACTTTAAAATCCCCACTTTTTAAAATTGGTGAGAAATCGGTTTTTGATTTAAAACAAAATGAAATTCCAATAACATGA", "aa": "MKHKTKKITLLIAGITLTSLCTIFAITACSKQKSQPNPKIKPNKIIEKIDQAFFNEFKTKIEQKRTLPNIKITSTFKIPFEKNENTLKSPLFKIGEKSVFDLKQNEIPIT", "aa_hexdigest": "faff9454f65313395f0710d596cb0c53", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12591.84009999998, "isoelectric_point": 9.930597114562985 }, "id": "NKIECMJPJD_106", "locus": "GCA000828735_00070" }, { "type": "cds", "sequence": "contig_1", "start": 48227, "stop": 49855, "strand": "-", "frame": 2, "gene": null, "product": "UvrABC system protein A", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PGE6" ], "nt": "ATGAGTGAAAAAGTTTGTAAATCATGTAAAGGCAAAAAACTATCTTCACAAGCTTTATCGGTTTTAATTAATAAAATTAACATTATTGATTTTATTGAAAAAAACATTGATGAGGCGATTGATTTTTTATTACACTTAGATTTGAATGAAGCACAAACAAAAATTGCCAATCCAATTTTAAAAGAAGTTTTAGATCGTTTAGGATTTTTAAAAAATGTTGGTTTAGAATATTTAACTTTAGCTCGTCCTGCATCAAGTTTATCAGGGGGCGAAGCACAACGGATTCGTTTAGCCACTCAAATCGGCTCTAAACTAACAGGTATTTTATATGTACTTGATGAACCATCAATTGGTTTGCATCAACGCGATAACGATAAATTAATTAATACATTAAAAGAAATGCGTGATTTAGGTAATACAGTTATAGTTGTTGAACATGATGAAGAAACAATGCTAGCAGCTGATTATTTAATAGATATTGGTCCTCAAGCTGGAGTTAATGGTGGTTATGTAATAGCAGCTGGAACACCACAAGAAGTAATGCAAAATCCTAATTCACTAACAGGACAATATTTATCAAAACAAAAAGATATTTTAGTTCCTAAAACACGAAGAAGTGGTAATGGTCATAAAGTTATTTTAAAAGGTGCAAAACATAATAATTTAAAAAATGTTGATTTAACAATTCCTTTAGGTAAATTCATTTGTGTTACTGGAGTTTCAGGATCTGGTAAATCATCACTAATTTTAGAAACATTAGTTAAAGCTATTGAATACACTAATTTTAATCCTTTTGTAATTCCAGGCGAGTATAAAGATTTAATTGGCGCATCGAATGTTGATAAAATCGTTGTCGTTAATCAAGATGCAATTGGTAGAACTACGAGATCAAATCCAGCTACTTATGTTGGGGTATTTGATGATATTAGAACAGTATTTGAAAATACAATTGAAGCTAAAGCACGTGGCTATAGTAAAAGTCGTTTTTCTTTTAATATTAAAGGAGGCCGTTGTGAACGTTGTTGGGGGGATGGTACGATTCGCATTGAGATGCACTTTTTACCAGATGTTTATATAAGTTGTGAAGAATGTCATGGAAAACGTTATAACGATGAAACACTTCAAGTTAAATTTAAAGGTAAATCAATTTATGATGTACTAAAAATGCCAATCGATGAAGCTTTAGTATTTTTTGAAAATTATCCTGGTATTCATCGTAAACTACAATTATTAGTTGATGTTGGTTTAGGATATTTAGAGTTAGGAGCCTCTTCAACTTCATTATCAGGGGGCGAAGCACAACGTATTAAGTTAGCTAAGTTTTTACAACGTAAGCCAACAGGAAAAACATTATTTGTTTTAGATGAACCAACAACAGGACTGCATATTGATGATGTAGCTAAATTAATTAAAATTTTAGATAAAATTGTTGATGGAGGAGACACTGTTTTAGTTATAGAACACAATCTTGATTTAATTAAGGTTGCAGATTATATTATTGATATTGGCCCTGAAGGTGGTAATAAAGGTGGTAAAATTATAGCTACTGGTACTCCAGAGCAATTACTAAGTAAAAAGGACATATCTTATACTGCTCAATATTTAGAAAAGTATTTAAAAAAGAACTAA", "aa": "MSEKVCKSCKGKKLSSQALSVLINKINIIDFIEKNIDEAIDFLLHLDLNEAQTKIANPILKEVLDRLGFLKNVGLEYLTLARPASSLSGGEAQRIRLATQIGSKLTGILYVLDEPSIGLHQRDNDKLINTLKEMRDLGNTVIVVEHDEETMLAADYLIDIGPQAGVNGGYVIAAGTPQEVMQNPNSLTGQYLSKQKDILVPKTRRSGNGHKVILKGAKHNNLKNVDLTIPLGKFICVTGVSGSGKSSLILETLVKAIEYTNFNPFVIPGEYKDLIGASNVDKIVVVNQDAIGRTTRSNPATYVGVFDDIRTVFENTIEAKARGYSKSRFSFNIKGGRCERCWGDGTIRIEMHFLPDVYISCEECHGKRYNDETLQVKFKGKSIYDVLKMPIDEALVFFENYPGIHRKLQLLVDVGLGYLELGASSTSLSGGEAQRIKLAKFLQRKPTGKTLFVLDEPTTGLHIDDVAKLIKILDKIVDGGDTVLVIEHNLDLIKVADYIIDIGPEGGNKGGKIIATGTPEQLLSKKDISYTAQYLEKYLKKN", "aa_hexdigest": "ec6bc1385cd1cfef4a00ebea0d33877a", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0PGE6", "query_cov": 0.9317343173431735, "subject_cov": 0.9960552268244576, "identity": 0.6629999999999999, "score": 683.0, "evalue": 2.95e-242, "valid": false, "gene": "uvrA", "product": "excinuclease ABC subunit UvrA", "uniref50_id": "UniRef50_A0A3B0PGE6", "kegg_orthology_id": "K03701", "go_ids": [ "GO:0003677", "GO:0006289", "GO:0009380", "GO:0016887" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3B0PGE6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PGE6" ], "product": "UvrABC system protein A" }, "genes": [], "id": "NKIECMJPJD_107", "locus": "GCA000828735_00071" }, { "type": "cds", "sequence": "contig_1", "start": 50099, "stop": 51058, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF17760.6" ], "nt": "ATGGACAAAATTATCATTAAAGGCGCTAGAGAAAATAATTTAAAAAATATTGATCTAGAAATTCCTAAAAATAAACTAGTTGTTATTACAGGTGTTTCAGGATCAGGTAAATCATCGCTTGCTTTTGATACGATTTTTGCTGAAGGAAAACGTCGTTATTTTGAATCACTTTCAAGTTATGCCCGCCAGTTTTTAGGTGGAAATGATAAAGCTGATGTTGAAAGTATTGAAGGTTTATCACCAACCATTGCTGTTGATCAAAAATCAACAAATCAAAATCCAAGATCAATTGTTGGGACAATTACAGAAATCTATGATTATTTACGAGTTTTATTTGCTCGTGTTGGAACACCTTTTTGTCCAAATGGGCATGGTCAAATTAAATCACAAACTCCAAAACAAATTGCTGATTTCTTATTTAGCTTATCACCTAAAAGTAAAATACAAATTCTTGCACCAATTGAAATTAAAAAAGGTTATAAAATTAATGATGTTTTAAATAATTTAAGAAATCAAGGTTATTTAAGAGTTTTAGTTAATAATGAAGTTTATCAATTAGATGAAAATTTGCCTCAATTTTTAGATAACAAAAAAACAGATGTTGCAATCATTGTTGATCGTTTAATTTTAAATATTGATCACCAAACAAAAACACGAGCACTAGATGCTATTGAATTTGCTTTAAATTATAGTGGGGGAGAAATTGCATTTAAGGTTAATGATGATATTCATTACTTTACACAAAACGATGTTTGTCAAGTTTGTGGTTTTAAAATTAAAGAAATTGAACCAACATTATTTTCATTTAATTCACCAATTGGTGCTTGTGAACAGTGTAAAGGGCTAGGATATAATTATGTTCCTGATGAGCGTAAAATGATCCCTAATCCAAATTTATCAATTAACGAAGGCGGTTTAGATTATTTTAAAAACACTGTTAATACAACTAATCTTGATTGA", "aa": "MDKIIIKGARENNLKNIDLEIPKNKLVVITGVSGSGKSSLAFDTIFAEGKRRYFESLSSYARQFLGGNDKADVESIEGLSPTIAVDQKSTNQNPRSIVGTITEIYDYLRVLFARVGTPFCPNGHGQIKSQTPKQIADFLFSLSPKSKIQILAPIEIKKGYKINDVLNNLRNQGYLRVLVNNEVYQLDENLPQFLDNKKTDVAIIVDRLILNIDHQTKTRALDAIEFALNYSGGEIAFKVNDDIHYFTQNDVCQVCGFKIKEIEPTLFSFNSPIGACEQCKGLGYNYVPDERKMIPNPNLSINEGGLDYFKNTVNTTNLD", "aa_hexdigest": "f7ba15b8bd433617c73ec53a1901f4d3", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI00261747A6", "query_cov": 0.9529780564263323, "subject_cov": 0.95, "identity": 0.513, "score": 300.0, "evalue": 7.58e-98, "valid": false, "product": "ATP-binding cassette domain-containing protein", "uniref50_id": "UniRef50_A0A9D2IF50", "kegg_orthology_id": "K03701" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF17760.6", "name": "UvrA_inter", "length": 112, "aa_cov": 0.3510971786833856, "hmm_cov": 0.9732142857142857, "evalue": 1.489517748631295e-28, "score": 94.45779418945312, "start": 130, "stop": 241 } ], "seq_stats": { "molecular_weight": 35712.21819999999, "isoelectric_point": 6.360192298889159 }, "id": "NKIECMJPJD_108", "locus": "GCA000828735_00072" }, { "type": "cds", "sequence": "contig_1", "start": 51284, "stop": 51847, "strand": "+", "frame": 2, "gene": null, "product": "putative protein UU101", "db_xrefs": [ "RefSeq:WP_006689247.1", "SO:0001217", "UniParc:UPI00016C021B", "UniRef:UniRef100_A0AAC9T302", "UniRef:UniRef50_Q9PR43", "UniRef:UniRef90_Q9PR43" ], "nt": "ATGGCTAAATACATTAAAACAGGTGTTTCTTACATTAATTTAGACAACGCACGTACAATTAATATTCTTCCAGAAGATATTGATTCATATTTAGAACTTGGTGGAGATGAAGCTTATAAAACAAGCGATCTAGGATCAGAGTTGTATATTAATTATGCTGATTTTGAATCAACAAACATTTTATTTGATCTTAAAAAAGAAGAATTACAAGCAAAAATTGATGCTTTTTTAATTTCTAATGATACAGTATTAGATTTATCAGAAGTTTTCTTAGATATTCATTTTAGTGAAGATGATGATTATGAAGAAGATTGCTGTTGTGAAGATGAATGCTGTAGCGATGAAGAAAATGAAGCTTGTTGTAATTCAGAAGTAAAAGTTGAAGAAGAATGCTGTGGCGGAGCTAAAGATGATTGTTGTGGTGGTCACGAACATGAACATGAAGTTTGCTGTGACTCGGAAACAAAAACATCAGAAACCCAAGAAGAATGTTGTGGTGGAACTAAAGATGATTGCTGTGGTGGTCACGAACATGAACACCATCATCACAGTCACCAACATTAA", "aa": "MAKYIKTGVSYINLDNARTINILPEDIDSYLELGGDEAYKTSDLGSELYINYADFESTNILFDLKKEELQAKIDAFLISNDTVLDLSEVFLDIHFSEDDDYEEDCCCEDECCSDEENEACCNSEVKVEEECCGGAKDDCCGGHEHEHEVCCDSETKTSETQEECCGGTKDDCCGGHEHEHHHHSHQH", "aa_hexdigest": "b2b59f19592c0430233a3a75d4f65218", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI00016C021B", "ncbi_nrp_id": "WP_006689247.1", "uniref100_id": "UniRef100_A0AAC9T302", "db_xrefs": [ "SO:0001217", "UniParc:UPI00016C021B", "RefSeq:WP_006689247.1", "UniRef:UniRef100_A0AAC9T302" ] }, "ips": { "uniref100_id": "UniRef100_A0AAC9T302", "uniref90_id": "UniRef90_Q9PR43" }, "psc": { "uniref90_id": "UniRef90_Q9PR43", "product": "Uncharacterized protein UU101", "uniref50_id": "UniRef50_Q9PR43" }, "pscc": { "uniref50_id": "UniRef50_Q9PR43", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR43" ], "product": "Uncharacterized protein UU101" }, "genes": [], "id": "NKIECMJPJD_109", "locus": "GCA000828735_00073" }, { "type": "cds", "sequence": "contig_1", "start": 51885, "stop": 52214, "strand": "-", "frame": 1, "gene": "hsdM", "product": "site-specific DNA-methyltransferase (adenine-specific)", "db_xrefs": [ "COG:COG0286", "COG:V", "SO:0001217", "UniRef:UniRef50_Q9PR44", "UniRef:UniRef90_Q9PR44" ], "nt": "ATGAAAATAACCTTAATTAAAGAAAAAGGTTTTTATATTAAGCCGTCATTTTTGTTTGCTAATATTGTTAAAAATGCTGAAAAAAATGAAAATTTAAATGAAACTTTGGAAACTGTTTTTAACAATATTGAAGGATCATCCTCTGGTTATGATTCTGAAAAAAATTTAAAAGGGTTATTTAATGATATTGATGTTAATAATAGTCGTTTAGGAAGTACTACACAAGAAAGAAATAAAAGACTAGCAAGAATTTTAATTAGAATTATATATAAAAATATGGATTTATTAATTTTAAAACCATATATAAATAAAAAAGAGATTCCTAATTAA", "aa": "MKITLIKEKGFYIKPSFLFANIVKNAEKNENLNETLETVFNNIEGSSSGYDSEKNLKGLFNDIDVNNSRLGSTTQERNKRLARILIRIIYKNMDLLILKPYINKKEIPN", "aa_hexdigest": "c06521abe5d973c6eca21c4dbdd0f354", "start_type": "ATG", "rbs_motif": null, "genes": [ "hsdM" ], "truncated": "3-prime", "pseudogene": { "start": 51885, "stop": 52448, "observations": { "mutation": [ 52361, 52400 ], "stop": [ 52361, 52400 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PR44", "query_cov": 1.0, "subject_cov": 0.5828877005347594, "identity": 1.0, "score": 211.0, "evalue": 5.18e-68, "gene_start": 1, "gene_end": 109, "reference_start": 79, "reference_end": 187, "reference_sequence": "MDNKKEIERNNLHATIWKIADELRGAIDGWDFKQYVLGILFYRYISENLTKYINDNEHKIGDHDFNYETCNDDDVDEEMKITLIKEKGFYIKPSFLFANIVKNAEKNENLNETLETVFNNIEGSSSGYDSEKNLKGLFNDIDVNNSRLGSTTQERNKRLARILIRIIYKNMDLLILKPYINKKEIPN" }, "paralog": false, "identity": 0.9893048128342246, "score": 371.0, "evalue": 1.11e-133, "description": "Internal stop codon at 52361, 52400. Nonsense mutation around 52361, 52400.", "psc": { "uniref90_id": "UniRef90_Q9PR44", "gene": "HsdM", "product": "site-specific DNA-methyltransferase (adenine-specific)", "uniref50_id": "UniRef50_Q9PR44", "cog_id": "COG0286", "cog_category": "V", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PR44", "UniRef:UniRef50_Q9PR44", "COG:COG0286", "COG:V" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PR44", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR44" ], "product": "site-specific DNA-methyltransferase (adenine-specific)" } }, "id": "NKIECMJPJD_110", "locus": "GCA000828735_00074" }, { "type": "cds", "sequence": "contig_1", "start": 52589, "stop": 53392, "strand": "-", "frame": 2, "gene": null, "product": "restriction endonuclease subunit S", "db_xrefs": [ "EC:3.1.21.3", "KEGG:K01154", "SO:0001217", "UniRef:UniRef50_UPI00017250EC", "UniRef:UniRef90_UPI00017250EC" ], "nt": "ATGAATAAAATTGAAAAATTAATTAATGAGTTCTGCCCTAATGGAGTGGAGTTTAAAAAATTAAAAAATATAATTACAGTGGCTCCTAAATCACCTTTTGGAGTTACAAAACTTTTAAAAATGGAAAAGGGAAATTATTTAACAATAACATCAGGGAAGAAATCATTTTATGTTGACAATTTTTTAGTTGATGGAGAATATATATTTGTTAATGATGGCGGTCAAGCAGATATAAAATATAATTTTGGAAAAACAATGTATTCTGATCATATATTCGCATTCAAAGTAAATGAATATAACACAAAATATCTTTACTTCTATCTATTAAATATTTCTAATTTTATTAATAAAAAATTGTTTATAGGTAGTACATTAAAAAATTTAAATAAAAAAGAATTTTTAAATCTTGCAATCCCTATTCCGCCCATTTCAATCCAAAATAAAATTGTTGAAATATTAGACAAGTATACAGAATTAGAAACAGAATTAGAAACAGAATTAGAAACAGAATTAGAACTTAGAAATAAGCAGTATATATATTATCGCAATGAATTACTAAACTTTAATAAAAATCAAACATTATTAAAGAAAATAATGGACAGTGATGATATAAAATCTATTGATCATAAAATTGAGTTTAAAAAGATTGAATATGTGTGTGAAATAAAAAGAGGTCAAGTATACTCTAAGGAATTTATTAATTCTAATAAAGGAAATTATCCAGTATATTCATCACAAAGTTTAAATGATGGTGTTTTAGGAAATATTAATAAATATGATTTTGATGGTGAATATGTTACTTGA", "aa": "MNKIEKLINEFCPNGVEFKKLKNIITVAPKSPFGVTKLLKMEKGNYLTITSGKKSFYVDNFLVDGEYIFVNDGGQADIKYNFGKTMYSDHIFAFKVNEYNTKYLYFYLLNISNFINKKLFIGSTLKNLNKKEFLNLAIPIPPISIQNKIVEILDKYTELETELETELETELELRNKQYIYYRNELLNFNKNQTLLKKIMDSDDIKSIDHKIEFKKIEYVCEIKRGQVYSKEFINSNKGNYPVYSSQSLNDGVLGNINKYDFDGEYVT", "aa_hexdigest": "ab997238ff58fb07b585aaf3405211de", "start_type": "ATG", "rbs_motif": "AGxAG", "psc": { "uniref90_id": "UniRef90_UPI00017250EC", "query_cov": 1.0, "subject_cov": 0.898989898989899, "identity": 0.978, "score": 495.0, "evalue": 3.98e-176, "valid": true, "product": "restriction endonuclease subunit S", "ec_ids": [ "3.1.21.3" ], "uniref50_id": "UniRef50_UPI00017250EC", "kegg_orthology_id": "K01154" }, "pscc": { "uniref50_id": "UniRef50_UPI00017250EC", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00017250EC" ], "product": "restriction endonuclease subunit S" }, "genes": [], "id": "NKIECMJPJD_111", "locus": "GCA000828735_00075" }, { "type": "cds", "sequence": "contig_1", "start": 53396, "stop": 54442, "strand": "-", "frame": 2, "gene": "hsdM", "product": "type I restriction-modification system subunit M", "db_xrefs": [ "COG:COG0286", "COG:V", "EC:2.1.1.72", "GO:0003677", "GO:0009007", "GO:0009307", "KEGG:K03427", "RefSeq:WP_006689008.1", "SO:0001217", "UniParc:UPI00000D4017", "UniRef:UniRef100_Q9PR46", "UniRef:UniRef50_Q9PR46", "UniRef:UniRef90_Q9PR46" ], "nt": "GTGTTCGGTGATGCTTATGAATACTTAATGAGTATGTATGCTGCAAATGCAGGTAAATCAGGGGGAGAATTTTTCACCCCTCAAGAAGTTAGTGAACTATTAGTAGAATTAACATTAATTGATTTTAATAATGAAAATAAAGATGTTAGAAGAAAAATTGGAAAAGTTTATGATCCATGTTGTGGTTCTGGATCATTACTTTTAAAGTATGCAAAATTAAATGAAGGAGTTAAATTTTATGGTCAAGAAATTAATCTTACTACGTATAATTTAGCAAGAATTAATATGTTTTTGCACAATATAGGTTATGATAAATTTGATATCAAATTAGGTGATACATTACTAGATCCTAAACATAATGATGATAAACCTTTTGATGCAATTGTTTCAAATCCTCCATATTCAACAAAATGGGAGGGAAAATCAAATCCTTTATTAGCTAACGATGAGCGATTTCATGTTACTCAATTAGCACCTAAAGGAAAAGCTGATTTTGCTTTTGTATTGCATATACTTCATAATTTATCTTCTAGTGGAACAGCAGCTATAGTTATGTTTCCAGGAACTTTATATCGAGATCATGCAGAACAAGATATTAGAAAATATTTAGTTGATAATGTTAATGTTGTAGATAGTGTGATTCAATTACCAGATAATCTGTTTTTTGGAACAAGTATTTCAACATGTATTATAGTTTTAAGAAAAAACAAAAACAACAATGATAATGCTAATGGTATCTTGTTTGTTGATGCATCAAAAGAATTTGTTAAATCTGGTATCAAAAACAAATTAACCAATGCTAATATTAAAAAAATAGTTGATACGATTCGTTTCAAAAAAGAAGTAACTTATTTTTCAAAACTTGTATCACGAGAAGAAGTTAAAAATAAAAACTATAATTTATCAGTTAACACATATGTGGAAAAAGAAGACACGTCAGAAAAAATTGATATTAAATTATTAAATATGCAAATTAAAGAAATTGTGGCTAAAATCGAAAAGTTAAGAAAAGAAATTGATGAAATTGTTTTAGAATTAGAAGAATAA", "aa": "MFGDAYEYLMSMYAANAGKSGGEFFTPQEVSELLVELTLIDFNNENKDVRRKIGKVYDPCCGSGSLLLKYAKLNEGVKFYGQEINLTTYNLARINMFLHNIGYDKFDIKLGDTLLDPKHNDDKPFDAIVSNPPYSTKWEGKSNPLLANDERFHVTQLAPKGKADFAFVLHILHNLSSSGTAAIVMFPGTLYRDHAEQDIRKYLVDNVNVVDSVIQLPDNLFFGTSISTCIIVLRKNKNNNDNANGILFVDASKEFVKSGIKNKLTNANIKKIVDTIRFKKEVTYFSKLVSREEVKNKNYNLSVNTYVEKEDTSEKIDIKLLNMQIKEIVAKIEKLRKEIDEIVLELEE", "aa_hexdigest": "b0c8788a6e26e2726c056ff7a5012a36", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00000D4017", "ncbi_nrp_id": "WP_006689008.1", "uniref100_id": "UniRef100_Q9PR46", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000D4017", "RefSeq:WP_006689008.1", "UniRef:UniRef100_Q9PR46" ] }, "ips": { "uniref100_id": "UniRef100_Q9PR46", "uniref90_id": "UniRef90_Q9PR46" }, "psc": { "uniref90_id": "UniRef90_Q9PR46", "gene": "HsdM", "product": "type I restriction-modification system subunit M", "ec_ids": [ "2.1.1.72" ], "uniref50_id": "UniRef50_Q9PR46", "cog_id": "COG0286", "cog_category": "V", "kegg_orthology_id": "K03427", "go_ids": [ "GO:0003677", "GO:0009007", "GO:0009307" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PR46", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR46" ], "product": "site-specific DNA-methyltransferase (adenine-specific)" }, "genes": [ "hsdM" ], "id": "NKIECMJPJD_112", "locus": "GCA000828735_00076" }, { "type": "cds", "sequence": "contig_1", "start": 54521, "stop": 54805, "strand": "+", "frame": 2, "gene": null, "product": "Type I restriction enzyme S protein", "db_xrefs": [ "EC:3.1.21.3", "KEGG:K01154", "SO:0001217", "UniRef:UniRef50_A0AAC9T2H6", "UniRef:UniRef90_Q9PR47" ], "nt": "TTGAGAAAAATTACTAAAAAACATGTAAATCAAGCATCAGGAAATCCAAAATTAATGAGTAATGTTATGCAAGAAATCATAATCCCTATTCCGCCCATTTCAATCCAAAATAAAATTGTTGAGATTTTAGATAAATTGGAAACATATACGAAAGATATTAATACTGGACTTCCTCTTGAAATCGAACAACGTAAAAAACAATACGAATATTATCGTAATAAATTATTAGACTTTGATAATATAGCACGAGAGAGAGAGAGAGAGCTAAGTAGGGATTACATTTGA", "aa": "MRKITKKHVNQASGNPKLMSNVMQEIIIPIPPISIQNKIVEILDKLETYTKDINTGLPLEIEQRKKQYEYYRNKLLDFDNIARERERELSRDYI", "aa_hexdigest": "6d33e821ff0252d853c2017ad48dc03c", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_Q9PR47", "query_cov": 0.925531914893617, "subject_cov": 0.8613861386138614, "identity": 0.9890000000000001, "score": 168.0, "evalue": 1.45e-52, "valid": true, "product": "Type I restriction enzyme S protein", "ec_ids": [ "3.1.21.3" ], "uniref50_id": "UniRef50_A0AAC9T2H6", "kegg_orthology_id": "K01154" }, "pscc": { "uniref50_id": "UniRef50_A0AAC9T2H6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T2H6" ], "product": "Type I restriction modification DNA specificity domain-containing protein" }, "genes": [], "id": "NKIECMJPJD_113", "locus": "GCA000828735_00077" }, { "type": "cds", "sequence": "contig_1", "start": 54926, "stop": 55447, "strand": "+", "frame": 2, "gene": null, "product": "Type I restriction enzyme S protein", "db_xrefs": [ "EC:3.1.21.3", "KEGG:K01154", "RefSeq:WP_010891674.1", "SO:0001217", "UniParc:UPI00000D4015", "UniRef:UniRef100_Q9PR48", "UniRef:UniRef50_Q9PR48", "UniRef:UniRef90_Q9PR48" ], "nt": "TTGTCATTTAAGCATATATCTGCAAACGAATTAAAACGATATGAAAAGTGTAATTTTGGTAATGTTAAACTTCTTTCAACAGGATTGTATGATGGATATATAAAATATAATGAAAATGATAATAATATTAATTATGGCGAAATAATTGCTTTGCCATCTGGTGGTTCTCCAATAATAAAATACTATAATGGTTATTTCATAGATAGTTTAAATATAATTTTTTCACAAAAAAACAAAAAAGAATGTAATTTAAAATTTATTTACTATTTTTTGATAGCAAATAAAATGCTTATTGAAGAAAATTATCGTGGTGCATCTGTAAAACATCCAAACATGATAGAGATTATTGAATTATTAATTCCTATCCCTCACATTTCAATCCAAAATAAAATTGTTGAAATCCTAGATAAATTAGAAGCATATACAAAAGATATTGGCGTAGGATTACCTTTTGAAATCAAACAACGCAAAAAACAATATGAATACTATCGAAATAAATTATTAGATTTTAAAAAATATTAA", "aa": "MSFKHISANELKRYEKCNFGNVKLLSTGLYDGYIKYNENDNNINYGEIIALPSGGSPIIKYYNGYFIDSLNIIFSQKNKKECNLKFIYYFLIANKMLIEENYRGASVKHPNMIEIIELLIPIPHISIQNKIVEILDKLEAYTKDIGVGLPFEIKQRKKQYEYYRNKLLDFKKY", "aa_hexdigest": "64034768b295b250ee5c8c9982a30661", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00000D4015", "ncbi_nrp_id": "WP_010891674.1", "uniref100_id": "UniRef100_Q9PR48", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000D4015", "RefSeq:WP_010891674.1", "UniRef:UniRef100_Q9PR48" ] }, "ips": { "uniref100_id": "UniRef100_Q9PR48", "uniref90_id": "UniRef90_Q9PR48" }, "psc": { "uniref90_id": "UniRef90_Q9PR48", "product": "Type I restriction enzyme S protein", "ec_ids": [ "3.1.21.3" ], "uniref50_id": "UniRef50_Q9PR48", "kegg_orthology_id": "K01154" }, "pscc": { "uniref50_id": "UniRef50_Q9PR48", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR48" ], "product": "Type I restriction enzyme S protein" }, "genes": [], "id": "NKIECMJPJD_114", "locus": "GCA000828735_00078" }, { "type": "cds", "sequence": "contig_1", "start": 55468, "stop": 55707, "strand": "+", "frame": 1, "gene": null, "product": "type I restriction endonuclease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00307E52CF", "UniRef:UniRef90_UPI00307E52CF" ], "nt": "ATGGAAAATAAGGTACTATTAGCAGAACATGAAAATTCAACGGTTGTTGCTGAATTTAAATCAATTAAAGTTCATTCGAATAAATTTGAAAGTGAAGCTGAATTAGAAGAAACATTTATTAAATTATTAATTGCACAAGGTTATGAATACTTACCTATTAAAAATAATAATGATTTAAAAAATAATTTAAGAAAACAAATCGAAAAACTTAATAATTATCAATTTACTAATAATGAATGA", "aa": "MENKVLLAEHENSTVVAEFKSIKVHSNKFESEAELEETFIKLLIAQGYEYLPIKNNNDLKNNLRKQIEKLNNYQFTNNE", "aa_hexdigest": "50aa796711ea9af7342e30f457eb02ef", "start_type": "ATG", "rbs_motif": "AGGAG(G)/GGAGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 55468, "stop": 55773, "observations": { "mutation": [ 55705 ], "stop": [ 55705 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00307E52CF", "query_cov": 1.0, "subject_cov": 0.7452830188679245, "identity": 0.975, "score": 146.0, "evalue": 4.47e-44, "gene_start": 1, "gene_end": 79, "reference_start": 1, "reference_end": 79, "reference_sequence": "MENKILLAEHENSTVVAEFKSIKVHSNKFESEAELEEAFIKLLIAQGYEYLPIKNNNDLKNNLRKQIEKLNNYQFTNNEWESFLSTYLLNPNDGIEARSKKNSWRL" }, "paralog": false, "identity": 0.9702970297029703, "score": 196.0, "evalue": 3.2e-66, "description": "Internal stop codon at 55705. Nonsense mutation around 55705.", "psc": { "uniref90_id": "UniRef90_UPI00307E52CF", "product": "type I restriction endonuclease", "uniref50_id": "UniRef50_UPI00307E52CF", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00307E52CF", "UniRef:UniRef50_UPI00307E52CF" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00307E52CF", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00307E52CF" ], "product": "type I restriction endonuclease" } }, "id": "NKIECMJPJD_115", "locus": "GCA000828735_00079" }, { "type": "cds", "sequence": "contig_1", "start": 55930, "stop": 56193, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTCTATTTTGATTAATGGACTTCCATTAGTACATATTGAACTAAAAAGACGAGGAACTAATCTTAAAGAAGCTTTTAATCAAATTAAAAGATATGCAAATCATTCGTTTACAGATTTATATCTTTACACGCAAATTTATGTTATTTCTAATGGTACACAAACTAAATATTATTCAAATACAACTAGAGAAACTTCTATAAAAGAAAGAAATAATACAAACTCTAAAAAAACAAGTAATTCTTTTGAATTTACATCATACTGA", "aa": "MSILINGLPLVHIELKRRGTNLKEAFNQIKRYANHSFTDLYLYTQIYVISNGTQTKYYSNTTRETSIKERNNTNSKKTSNSFEFTSY", "aa_hexdigest": "2b2b8d68e2ab7e349de56445efc26a7c", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10207.334999999992, "isoelectric_point": 9.7413179397583 }, "id": "NKIECMJPJD_116", "locus": "GCA000828735_00080" }, { "type": "cds", "sequence": "contig_1", "start": 56314, "stop": 56427, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTTTAAGACCATACCAAATTGTTGCAACTGAAAGAATTATTAATCGAATTAAGATTTCTTTAAATGATAAAAAATTATTAGGAACTACAGGCGCTGGTGGTTATATTTGA", "aa": "MVLRPYQIVATERIINRIKISLNDKKLLGTTGAGGYI", "aa_hexdigest": "c881ed184ac004cb6bbf9f1f3280a982", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4116.870700000001, "isoelectric_point": 10.160878181457516 }, "id": "NKIECMJPJD_117", "locus": "GCA000828735_00081" }, { "type": "cds", "sequence": "contig_1", "start": 56497, "stop": 57621, "strand": "+", "frame": 1, "gene": null, "product": "DEAD/DEAH box helicase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3E2CM26" ], "nt": "GTGGATAAGGTTCTATTTGTTGTTGATCGAAAAGATTTAGACTATCAAACAACAAAAGAATATGAAAAGTATTCTAAAGGATGTGTTAGTGCTAATACAAATGTTAATGAACTAGCTAAGCAAATTGGAAATCAAGAGAATAAAATTATTATTACAACCATTCAAAAACTTGGCATATTCATTAGTAAAAATCCAAAACATCCTATTTATTCTAAAAATGTTGTTCTTGTTTTTGATGAATGTCATCGTTCTCAATTTGGTGATTTACATAAAAAAATTACTAAAAAATCTTTTAAAAAATATATGATTTTTGGGTTTACAGGCACACCTATTTTAGAAGAAAATAGTAATGTCAAAAATAAAAATATTTTCTTTACAACAGAGCATCTTTTTGGAGCTTGTTTACATAAATATACAATTGATAAAGCTATTCGTGATGGTAATGTTTTAGGTTTTAGAACGGAGTATTATAAAACACTTAGCGAAAAAGATGACATTGACGATGAAACATCATCAACTGGAATAGATAAAAAAGAAGCTTTGGAAAATCCGATTCGTATTTCTGAAATTGTTGATTTTATTTTAAAAAACTATAATCGTTTAACCAAAAGAAATGAAGAATTTTTTGAACATAATAAGATTGCTAATATAAATGAAGTTATTACTAGCAGAAATAAATTAAGCGAAAATAAAGTATTAACAACACTAAATGGATTTAATTCTATTCTAGCTTGCTCTTCTATTAATGCAGCTAAAATATATTATTCAGAATTTAAAAAACAACAACAAAAATTTCCTCAAGAAGAAAAATTAAAAATTGGATTAATTTATAGTTATTTACCAAGCGAACAAGAAAGTAATGATAATGAACTTTCTAATTTTGAACTATTAGATGAGAATAACGAAAACACTAATCAACTTGATTTAGATTCACGTAATTTTCTTGATGAAGCGATTAAAGATTATAATGAAATGTTTGGGACATCATACTCAACAGATAATGCTAATTTTAATAATTACTATAAAGACTTTAGCTTGAGATTAAAGGATCGAAGCTTAGATATAGCAATCGTTGTTAATATGTTTTTAACAGGTTTTGATGCAACAACTTTAAATACATTATGA", "aa": "MDKVLFVVDRKDLDYQTTKEYEKYSKGCVSANTNVNELAKQIGNQENKIIITTIQKLGIFISKNPKHPIYSKNVVLVFDECHRSQFGDLHKKITKKSFKKYMIFGFTGTPILEENSNVKNKNIFFTTEHLFGACLHKYTIDKAIRDGNVLGFRTEYYKTLSEKDDIDDETSSTGIDKKEALENPIRISEIVDFILKNYNRLTKRNEEFFEHNKIANINEVITSRNKLSENKVLTTLNGFNSILACSSINAAKIYYSEFKKQQQKFPQEEKLKIGLIYSYLPSEQESNDNELSNFELLDENNENTNQLDLDSRNFLDEAIKDYNEMFGTSYSTDNANFNNYYKDFSLRLKDRSLDIAIVVNMFLTGFDATTLNTL", "aa_hexdigest": "d7573903a16ea6c5373601cdd9c22180", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0030771B9D", "query_cov": 1.0, "subject_cov": 0.9280397022332506, "identity": 0.511, "score": 345.0, "evalue": 1.15e-113, "valid": false, "product": "HsdR family type I site-specific deoxyribonuclease", "ec_ids": [ "3.1.21.3" ], "uniref50_id": "UniRef50_A0A3E2CM26", "kegg_orthology_id": "K01153" }, "pscc": { "uniref50_id": "UniRef50_A0A3E2CM26", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3E2CM26" ], "product": "DEAD/DEAH box helicase" }, "genes": [], "id": "NKIECMJPJD_118", "locus": "GCA000828735_00082" }, { "type": "cds", "sequence": "contig_1", "start": 57622, "stop": 58605, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF12008.13", "PFAM:PF22679.2" ], "nt": "TTGGACAAAAAACTAAAATATCATAGTTTAATTCAAGCAATGTCGCGAACCAATCGAATTCTTAATGGTGTTAAATCAAATGGTAATATTGTCTTTTTCATAAATTTAGAAGATGAAGTGAATGAAGCTTTAAGATTATTTTCATCAGAGAGCAATATTAATTCTTCGATCGCTATTTTAAGATCATTTAAAGAATATTATGAAGGATATTATAATGAAGATAATCAATATATACAAGGTTATCGAAACTTAGTAGAAGAATTAAAAAGTAACTTTAAAATTCCTTTTAATGGAACAAAAGAAGAGGAAAGAATTTTTATTAAACTTTTTGGTGATATTTTAAAATATAAAAATTTGCTTTCACAATTTAGTGAATTTGAACAAAACGATGATCTTATTGGTACAGAAATGCAAGATTATCAAAGTATATATATCGGGTTGCGTGATAAATATCAAGATAAAAAACGTATGGAAACAGATAGCATTGTTAGTGATCTTGATTTTAAAATTGAATTAATTAGTCAACAAGATATTGGTGTTGATTATATTCTTAAGTTAATGAATGAAAAGCTCAAAAAAGATCTAAAAGAAGTTAAATATCACGATCTAGAAAGAGATATTTTATCTAGTCTAAAAGCGCGAAAAAAGAAAGATTTAATTATTGAATTTGTTAATGAAATTAATAAACTACCTAATAAGGATGAAATTGATATTTTTAATGAATGGAGTAAGTTTATTAAAAATAAAAAAGAAAGTGAGCTTATAAAAATAATTAATGAAAATAAATTAGATAACAAAAAAACATTTGAATTTATGAAAAAAGCATTTGAAAATAATGAATTTAATGGAAATGGAGTAGATATTGTTAATTTAATGCCTCCTACCTCTTTTTTTAAAAAAAGTAATCAGAATAGTAATAGGTCTAAAATAAAAGAAAATGCTACTGAAAAATTACTCGAATATTTTGAAAAATTCCGTGGTTAA", "aa": "MDKKLKYHSLIQAMSRTNRILNGVKSNGNIVFFINLEDEVNEALRLFSSESNINSSIAILRSFKEYYEGYYNEDNQYIQGYRNLVEELKSNFKIPFNGTKEEERIFIKLFGDILKYKNLLSQFSEFEQNDDLIGTEMQDYQSIYIGLRDKYQDKKRMETDSIVSDLDFKIELISQQDIGVDYILKLMNEKLKKDLKEVKYHDLERDILSSLKARKKKDLIIEFVNEINKLPNKDEIDIFNEWSKFIKNKKESELIKIINENKLDNKKTFEFMKKAFENNEFNGNGVDIVNLMPPTSFFKKSNQNSNRSKIKENATEKLLEYFEKFRG", "aa_hexdigest": "6b2f817faf98d6b78ca8b453660f6a9d", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF12008.13", "name": "EcoR124_C", "length": 275, "aa_cov": 0.8134556574923547, "hmm_cov": 0.9381818181818182, "evalue": 6.099936219544193e-51, "score": 169.03977966308594, "start": 60, "stop": 325 }, { "id": "PF22679.2", "name": "T1R_D3-like", "length": 33, "aa_cov": 0.10091743119266056, "hmm_cov": 1.0, "evalue": 2.8132089995059835e-12, "score": 42.88441848754883, "start": 1, "stop": 33 } ], "seq_stats": { "molecular_weight": 38818.7324, "isoelectric_point": 6.325463676452637 }, "id": "NKIECMJPJD_119", "locus": "GCA000828735_00083" }, { "type": "cds", "sequence": "contig_1", "start": 59674, "stop": 59823, "strand": "+", "frame": 1, "gene": null, "product": "Conserved domain protein", "db_xrefs": [ "RefSeq:WP_012316997.1", "SO:0001217", "UniParc:UPI000165F8D6", "UniRef:UniRef100_A0A2C9DYI3", "UniRef:UniRef50_A0A2C9DYI3", "UniRef:UniRef90_A0A2C9DYI3" ], "nt": "GTGATTAATGATATTGCTGATTCTTTTGATTATAAAAATATATATGCAATAAAAATTTCAAATTTAATAGTTTTAACTCATAATTTTGATTTTTATAGAACAATATCATTGGGATTTGATGTTTGCAAAGAATCGCAATTTGTTGTATAA", "aa": "MINDIADSFDYKNIYAIKISNLIVLTHNFDFYRTISLGFDVCKESQFVV", "aa_hexdigest": "9aa218bb9e77aed41c5e89c6aa0e9626", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000165F8D6", "ncbi_nrp_id": "WP_012316997.1", "uniref100_id": "UniRef100_A0A2C9DYI3", "db_xrefs": [ "SO:0001217", "UniParc:UPI000165F8D6", "RefSeq:WP_012316997.1", "UniRef:UniRef100_A0A2C9DYI3" ] }, "ips": { "uniref100_id": "UniRef100_A0A2C9DYI3", "uniref90_id": "UniRef90_A0A2C9DYI3" }, "psc": { "uniref90_id": "UniRef90_A0A2C9DYI3", "product": "Conserved domain protein", "uniref50_id": "UniRef50_A0A2C9DYI3" }, "pscc": { "uniref50_id": "UniRef50_A0A2C9DYI3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2C9DYI3" ], "product": "Conserved domain protein" }, "genes": [], "id": "NKIECMJPJD_120", "locus": "GCA000828735_00084" }, { "type": "cds", "sequence": "contig_1", "start": 59935, "stop": 60063, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_006688744.1", "SO:0001217", "UniParc:UPI000165F8D5", "UniRef:UniRef100_A0A2C9DZ08", "UniRef:UniRef50_A0A2C9DZ08", "UniRef:UniRef90_A0A2C9DZ08" ], "nt": "ATGATCATTAATTTCCAAATGCTAATAGATTTATATAAAAATTATATTGGAAAAGAAAATATTCAATTACCAAGTAATTTTTCAAAAGAAGATATTGTGATGAAAAAAATTCAAAAAGTAGTTAAGTAA", "aa": "MIINFQMLIDLYKNYIGKENIQLPSNFSKEDIVMKKIQKVVK", "aa_hexdigest": "80f705ee5e85c02c2bf22264adca1632", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "ups": { "uniparc_id": "UPI000165F8D5", "ncbi_nrp_id": "WP_006688744.1", "uniref100_id": "UniRef100_A0A2C9DZ08", "db_xrefs": [ "SO:0001217", "UniParc:UPI000165F8D5", "RefSeq:WP_006688744.1", "UniRef:UniRef100_A0A2C9DZ08" ] }, "ips": { "uniref100_id": "UniRef100_A0A2C9DZ08", "uniref90_id": "UniRef90_A0A2C9DZ08" }, "psc": { "uniref90_id": "UniRef90_A0A2C9DZ08", "uniref50_id": "UniRef50_A0A2C9DZ08" }, "pscc": { "uniref50_id": "UniRef50_A0A2C9DZ08", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2C9DZ08" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5015.994300000002, "isoelectric_point": 9.45688343048096 }, "id": "NKIECMJPJD_121", "locus": "GCA000828735_00085" }, { "type": "cds", "sequence": "contig_1", "start": 60210, "stop": 60455, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTTGGATTTGGGAAAAAAGTTGATTTAAAAAAAGATGTTTATTTGAGTGCAGTTGCATTTAATTTTGGTTTAAACACTAACGATAGTGATCAAACAAACGTCTTAAATAATAATGATGAAATTAAATACAATCTAAATATTTTAAAAAAAGTAGTTATCACATTACACTTTACTAAGCCAATGTTAGATTTAAGTGATCCTAATCGCACTAAATTAGTTAATTCTTATACTTTTGTTGTTTAG", "aa": "MLGFGKKVDLKKDVYLSAVAFNFGLNTNDSDQTNVLNNNDEIKYNLNILKKVVITLHFTKPMLDLSDPNRTKLVNSYTFVV", "aa_hexdigest": "527f2c73e05b71538cb4f10a29f25569", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9209.513299999993, "isoelectric_point": 8.96376476287842 }, "id": "NKIECMJPJD_122", "locus": "GCA000828735_00086" }, { "type": "cds", "sequence": "contig_1", "start": 60795, "stop": 60905, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCTAATTCTATGAAAGCAATCATTGAAAATATATCATTAAGAAACGATCGTTTATTACCTAATAAATATGTTGCTCAATTATTAAATTGGTTATTTTATAAAAAATGA", "aa": "MPNSMKAIIENISLRNDRLLPNKYVAQLLNWLFYKK", "aa_hexdigest": "69a95f493ae9f351e3c18006d6a1318b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4336.131800000001, "isoelectric_point": 9.994614219665529 }, "id": "NKIECMJPJD_123", "locus": "GCA000828735_00087" }, { "type": "cds", "sequence": "contig_1", "start": 61368, "stop": 61907, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATTTTAAAAATAATTTAATTGCTGAAATTGGACAAATTAGAACCTTACTTGATAGTATTTCACTAGCTGAAATTAAAACTTATAAACCAATTATTAATAAATTATTAGAATTAATTTCTTTAATTGAAGCACGTTCTAAAAAAGACCCTAAAAATTATGGATTTATTGATGGTTTGGACGAACTATTTTCCTTCTTTTTAAATTTAAAACAAAATGATCTACCAAGTAGTATTAATTTTAAAATTGGTGATCAAAATTATACTTTATATGATTTAATTACTGAAGTTAAAAAAATTGCAAATAACTTAATTCCGCATGAAAACCAATATGACCCTAAAGACCAACAAATTATCAGTACCAATTATTTTTTAACAAAAATTAAAATCTTAGATTTGATTTCTTTAAATAAAAATGATCAACTTGATTATCACAAGGGTGTTGAAATGATTCTTAGTTTATTAAAAAAATTTCAAATAGCAATCCCTGAATTTATTTATAAAATTATTGATGAATTACTAATTCAAAATACTAATTGA", "aa": "MDFKNNLIAEIGQIRTLLDSISLAEIKTYKPIINKLLELISLIEARSKKDPKNYGFIDGLDELFSFFLNLKQNDLPSSINFKIGDQNYTLYDLITEVKKIANNLIPHENQYDPKDQQIISTNYFLTKIKILDLISLNKNDQLDYHKGVEMILSLLKKFQIAIPEFIYKIIDELLIQNTN", "aa_hexdigest": "6a20dac2b52888d4c299ebc4bd8b964f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 20810.90110000001, "isoelectric_point": 5.443436241149903 }, "id": "NKIECMJPJD_124", "locus": "GCA000828735_00088" }, { "type": "cds", "sequence": "contig_1", "start": 61923, "stop": 62489, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCGTTATTATTTTAATAATATTCGTGTTGAAAAACAAGTTACAAAGGTTAAAAACAATGCTAAACGTGGAGACCAAAATGCAGAAGAAAATTTTCCAAAAGAATTTTATTTCGGTGCTAATGATAATGAAAAATTTAGCTTTGGTACACACTCATACACATTAAATAATGAAATCACACAAGCTTCATATGAACAAGATATTTTTAGTTTAGATCATAAAATTAAAAACGAATTAAAAAAATTAAATGAACAACAAATTTTAAAAGATATTGAATATGCATTTACTAGTGGTTATGATGTAGCAATTGATTCCATTGATAGTATTTTTAACATTTTAAAAGGTGTTGCTAATGATTTAAATTTAAAAGAATTGTTTTTACAATCATCACAAGATTTTAGAAATTTAACTTATAACATTACACAAGAGAATAATTTGACTAATTTAATCACTTTAATTACATCAAATCAATCATTAGGGATGGTTATCGATGGTTTTAAACCAATTTTAAAAGAAATTATTTATAAGAATCCAAGCATTGACCAATCAGTTAAAAATACAATTTGA", "aa": "MRYYFNNIRVEKQVTKVKNNAKRGDQNAEENFPKEFYFGANDNEKFSFGTHSYTLNNEITQASYEQDIFSLDHKIKNELKKLNEQQILKDIEYAFTSGYDVAIDSIDSIFNILKGVANDLNLKELFLQSSQDFRNLTYNITQENNLTNLITLITSNQSLGMVIDGFKPILKEIIYKNPSIDQSVKNTI", "aa_hexdigest": "37a85810479a5ff3c66e2166c9015a33", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 21773.14480000001, "isoelectric_point": 5.28161563873291 }, "id": "NKIECMJPJD_125", "locus": "GCA000828735_00089" }, { "type": "cds", "sequence": "contig_1", "start": 63496, "stop": 64206, "strand": "-", "frame": 3, "gene": "rbbA", "product": "ABC transporter", "db_xrefs": [ "COG:COG1131", "COG:JV", "RefSeq:WP_006688981.1", "SO:0001217", "UniParc:UPI00000C1B95", "UniRef:UniRef100_Q9PR51", "UniRef:UniRef50_A0A449B6T2", "UniRef:UniRef90_Q9PR51" ], "nt": "ATGAATAATGAATTTATTTTAGAAGTTAAAAATTTAACAAAAATTTATAAAAAATCAAACCAAGGCGTTTTTGATTTAAGTTTTAATGTAAAACGTGGCGAAATTCATGCATTTATTGGTGAAAATGGATCAGGTAAAACAACAACTATTAAATCAATTATTAATGCATACACAAATTACAAAGGATCAATTTTAATTGATGGTTTTGATAATAAAACCCCAAAAAGTCATGAAAAAATTGGTTATGTGCCAGAAATTGCTTTATTTCCTAGTGAATTAACAACTTTTCAATATTTATATTCATTTGCTCGTTTATCAAATTTAAATAAAAAACAAGCTATTGAACGCATTCATAATTTTTTAGAAAAATTTAGAATTAGTGATTTAAAAAATAAAAAACCAATCAATTTTTCATCTGGACAAAAAAAGAAAGTAATTTTAATTCAAGCTTTATTACACGATCCAGAGCTAATTATTTTAGACGAACCAACAGCGAACTTAGACCCATCAGCACGTCATGAATTCAATAATATCTTAAAAGAATTGCACGAACAAAACAAAACCATTTTTATTTGTTCGCATATTTTAAAAGAAATGGATACTTACGTTGATAGTTTGACTTTAATTCACAAAGGCAAGCTAGTATATAGTGGTCATAAATATGATGCATTAGAGAAAATCTACTATGACAGTGTTTTAAAAAATAAATAA", "aa": "MNNEFILEVKNLTKIYKKSNQGVFDLSFNVKRGEIHAFIGENGSGKTTTIKSIINAYTNYKGSILIDGFDNKTPKSHEKIGYVPEIALFPSELTTFQYLYSFARLSNLNKKQAIERIHNFLEKFRISDLKNKKPINFSSGQKKKVILIQALLHDPELIILDEPTANLDPSARHEFNNILKELHEQNKTIFICSHILKEMDTYVDSLTLIHKGKLVYSGHKYDALEKIYYDSVLKNK", "aa_hexdigest": "891831325da65e8af51cec3ba8b67ace", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI00000C1B95", "ncbi_nrp_id": "WP_006688981.1", "uniref100_id": "UniRef100_Q9PR51", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1B95", "RefSeq:WP_006688981.1", "UniRef:UniRef100_Q9PR51" ] }, "ips": { "uniref100_id": "UniRef100_Q9PR51", "uniref90_id": "UniRef90_Q9PR51" }, "psc": { "uniref90_id": "UniRef90_Q9PR51", "gene": "RbbA", "product": "ABC transporter", "uniref50_id": "UniRef50_A0A449B6T2", "cog_id": "COG1131", "cog_category": "JV" }, "pscc": { "uniref50_id": "UniRef50_A0A449B6T2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449B6T2" ], "product": "ABC transporter ATP-binding protein" }, "genes": [ "rbbA" ], "id": "NKIECMJPJD_126", "locus": "GCA000828735_00090" }, { "type": "cds", "sequence": "contig_1", "start": 64272, "stop": 64529, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAATATAAAACACAATTAAAAATAAATTTAAACAATAAAAATTATTTAATTGGTGGTTTTAAAAGCTACCTACTTCATCAGATATTACCTCAAGAAAATGACGATGGCATAGATGAATTAAAAAAAGATAAAAAGATTCGTTACAGATATAGTTTAGAACAAGGTGGAAATTATTTATTCGATTATGTCCAACAAAATTATAGTTTTAAAAGAAAGCAACAAATTGTTTATAAAGAAGCTTACTGCGTAATTTGA", "aa": "MQYKTQLKINLNNKNYLIGGFKSYLLHQILPQENDDGIDELKKDKKIRYRYSLEQGGNYLFDYVQQNYSFKRKQQIVYKEAYCVI", "aa_hexdigest": "43e4198b986406cfc53ef1d792e33a04", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10308.673299999999, "isoelectric_point": 9.277596855163573 }, "id": "NKIECMJPJD_127", "locus": "GCA000828735_00091" }, { "type": "cds", "sequence": "contig_1", "start": 64695, "stop": 64838, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTATTAAAGAAGTATTAAACGTAAAATCGTTTAATACATATGCTAATAATTTATTTAAAACACTTGATCAAAAAGCAAATCAAAAACAAATCTTAGACTTAATTTCAAATAGTGTACAAAAATTTGATTTCAATAAATGA", "aa": "MIIKEVLNVKSFNTYANNLFKTLDQKANQKQILDLISNSVQKFDFNK", "aa_hexdigest": "e09f00152c9acbdb89a83108a22ee602", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5505.303900000001, "isoelectric_point": 9.523737144470214 }, "id": "NKIECMJPJD_128", "locus": "GCA000828735_00092" }, { "type": "cds", "sequence": "contig_1", "start": 65208, "stop": 65438, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTTACTATTGGAATTATTGCGAACTTAGTTAGTCAAGTTCAATCACAAGCCTTTTTAAGATTATTAAAACAAAAATCAACAAACAATATTAAAGCTTTTTATTTAAATAATAATTTAGATCAATATTATTTAATTAACACAGGTTTTTTTAATCATAAATTTAATGAAAATCAAAATTTAGAAATTATGAACGCTTATTTAAAAACTAGAAGTTTAGCAACTTTTTGA", "aa": "MLTIGIIANLVSQVQSQAFLRLLKQKSTNNIKAFYLNNNLDQYYLINTGFFNHKFNENQNLEIMNAYLKTRSLATF", "aa_hexdigest": "880eca236322c8c40a6d806ada88d393", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8863.098899999994, "isoelectric_point": 9.69728603363037 }, "id": "NKIECMJPJD_129", "locus": "GCA000828735_00093" }, { "type": "cds", "sequence": "contig_1", "start": 65676, "stop": 65993, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAAAAAAATAAATTTGAACAGCAGTTTAGCTTATATTGGTTTTTTAACACGCCTTATTATTGTAAAAAAAACAACATATCTTTTACCGATCATAACTTTATTAATTACATTAATTATAGGAATTGTTGTCGGCTGTGTTGTTAAGCAAAAAACACAATTTTTGATAATTTCATATGTAATGATCATGTTTAATTTATTAATGACAACAATTTTTGCTTCACTAAAAGCATTAAATATTTTTAAGGATTTTAGCGAAGATGGTATTGATATTTTAGTAATTTCTAAACCAATTAGCCGTAAAAACATTGTTTGA", "aa": "MNKKINLNSSLAYIGFLTRLIIVKKTTYLLPIITLLITLIIGIVVGCVVKQKTQFLIISYVMIMFNLLMTTIFASLKALNIFKDFSEDGIDILVISKPISRKNIV", "aa_hexdigest": "399f6e14d227f586fc6402c241144580", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11835.454799999983, "isoelectric_point": 9.944586753845215 }, "id": "NKIECMJPJD_130", "locus": "GCA000828735_00094" }, { "type": "cds", "sequence": "contig_1", "start": 66206, "stop": 66415, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02978.25" ], "nt": "ATGACTTTAAAAGAACGTCGTGATCCGCGCGTTTTTAAAAAAGAACCATCAAGACGCATACGGGTTTTAAAAGGATCAGGACGTTCGCCTGATGAATTAAATAAGTTATTAAAACAGTGGGAAGTGTCACGTGATAAAATGGCAGAACTTGGTAAAATGTTACAAAAAGGAAAAAATCCTTTTAGTAAATCTGGTGGGTTATTTAGTTAA", "aa": "MTLKERRDPRVFKKEPSRRIRVLKGSGRSPDELNKLLKQWEVSRDKMAELGKMLQKGKNPFSKSGGLFS", "aa_hexdigest": "b5bba1a0fe10a3b040f4d97952383c8f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02978.25", "name": "SRP_SPB", "length": 47, "aa_cov": 0.6811594202898551, "hmm_cov": 0.9574468085106383, "evalue": 9.348255739975718e-12, "score": 40.79910659790039, "start": 1, "stop": 47 } ], "seq_stats": { "molecular_weight": 8002.314199999999, "isoelectric_point": 10.807560539245607 }, "id": "NKIECMJPJD_131", "locus": "GCA000828735_00095" }, { "type": "cds", "sequence": "contig_1", "start": 66434, "stop": 66688, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02978.25" ], "nt": "ATGGCTGATCGAATTTTAGGTCTTGGTGATATCATGACTTTAGCAGAGAAAGCAGCTGATGTTATTGATGAAAAACAAGTTCGTGGTTCGATGCAACGAATGATGGCAGGAAAAATGGATTTAGAAGATTTAATGCGTCAAATGAGTCAAATTAGTAAATTAGGTAGTTTTTCAGGAATAGCTAAGATGATTCCTGGATTGAACAATATTTCTGAAAACCAAATTGGTGATGCTGAGAGCAAAATGAAAATATGA", "aa": "MADRILGLGDIMTLAEKAADVIDEKQVRGSMQRMMAGKMDLEDLMRQMSQISKLGSFSGIAKMIPGLNNISENQIGDAESKMKI", "aa_hexdigest": "c4c9e2694e695a49e21b0ed97258ab73", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02978.25", "name": "SRP_SPB", "length": 44, "aa_cov": 0.5238095238095238, "hmm_cov": 0.8181818181818182, "evalue": 4.387979153280858e-09, "score": 32.236419677734375, "start": 38, "stop": 81 } ], "seq_stats": { "molecular_weight": 9203.733099999994, "isoelectric_point": 5.295029640197753 }, "id": "NKIECMJPJD_132", "locus": "GCA000828735_00096" }, { "type": "cds", "sequence": "contig_1", "start": 66839, "stop": 67000, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00448.28" ], "nt": "TTGATAATTGTTGATACAGCTGGACGTTTACAAACAAACGAAGAGTTAATGCAAGAATTAGTAAATGTTAAAAAAACACTAAATCCTGATGAGGTTTTTTTAGTTGTTGATGCTATGGCAGGCCAAGACATTATTAACGTTGCTACAGAATTCAATAATTGA", "aa": "MIIVDTAGRLQTNEELMQELVNVKKTLNPDEVFLVVDAMAGQDIINVATEFNN", "aa_hexdigest": "aa6d8e26e94eef48d252fe29ddcbe451", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00448.28", "name": "SRP54", "length": 53, "aa_cov": 1.0, "hmm_cov": 1.0, "evalue": 1.8900834233540892e-18, "score": 62.37493896484375, "start": 1, "stop": 53 } ], "seq_stats": { "molecular_weight": 5905.684400000002, "isoelectric_point": 4.0500284194946286 }, "id": "NKIECMJPJD_133", "locus": "GCA000828735_00097" }, { "type": "cds", "sequence": "contig_1", "start": 67073, "stop": 67552, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00448.28", "PFAM:PF02881.24" ], "nt": "ATGTTCAAAGCGATGATAGGTAGTATTGTAAGTAAGCAAATGTCAAAAAAACTGAAAAATGCGACAATTGCTGAAGAAGATATAAAAGAATTATTAAGTGAAATAAGAATTGCATTATTAGATGCTGACGTTAATTTATTAGTTGTTAAAAAATTTATTAAAAACATCAAAGAGAAAACAGTTGGGTTGTATGTTGAAGCAAACCAAAAGCCAGCAGATCTTGTTTTAAAAATAATTAAAGATGAATTGGTTGAAATTTTAGGAAAAGAAAATAAACCAGTTAATACAGCTAAATCACAATTAAAAATTATGATGGTTGGTTTACAAGGTTCGGGTAAAACAACAACTACTGGTAAATTAGCTAATTATTTTAAAAATAAATACAATAAAAAACCATTATTAGTGGCGGCTGATATCTATCGCCCAGCAGCGATAGATCAATTAAGAACATTAGCAAAACAAGTCCGTGTAGATTTTTGA", "aa": "MFKAMIGSIVSKQMSKKLKNATIAEEDIKELLSEIRIALLDADVNLLVVKKFIKNIKEKTVGLYVEANQKPADLVLKIIKDELVEILGKENKPVNTAKSQLKIMMVGLQGSGKTTTTGKLANYFKNKYNKKPLLVAADIYRPAAIDQLRTLAKQVRVDF", "aa_hexdigest": "5b85e9b629315dc650c06659bbabd573", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_UPI00267073CB", "query_cov": 0.9119496855345912, "subject_cov": 0.9931506849315068, "identity": 0.517, "score": 143.0, "evalue": 4.05e-41, "valid": false, "product": "signal recognition particle receptor subunit alpha", "uniref50_id": "UniRef50_A0A533RPK5" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00448.28", "name": "SRP54", "length": 57, "aa_cov": 0.3584905660377358, "hmm_cov": 0.9824561403508771, "evalue": 3.495918314233674e-24, "score": 81.08291625976562, "start": 102, "stop": 158 }, { "id": "PF02881.24", "name": "SRP54_N", "length": 74, "aa_cov": 0.44025157232704404, "hmm_cov": 0.9594594594594594, "evalue": 4.013536247478076e-15, "score": 51.41391372680664, "start": 14, "stop": 83 } ], "seq_stats": { "molecular_weight": 17759.96669999999, "isoelectric_point": 9.784705162048336 }, "id": "NKIECMJPJD_134", "locus": "GCA000828735_00098" }, { "type": "cds", "sequence": "contig_1", "start": 67894, "stop": 68253, "strand": "-", "frame": 3, "gene": "recR", "product": "recombination mediator RecR", "db_xrefs": [ "COG:COG0353", "COG:L", "GO:0003677", "GO:0006281", "GO:0006310", "GO:0046872", "KEGG:K06187", "SO:0001217", "UniRef:UniRef50_Q9PR56", "UniRef:UniRef90_Q9PR56" ], "nt": "ATGATTGTTACCACAAACGAGGATTTAGAAAGAATCGAATCAGCTAACATTTATAATGGCCTATATCATATTACAAATGGTGAAATCTCTTTAAGGAAAAACGTTGTTATTGAACATACAAACATTAAGACTATTAAAGAGCGTGTTTTAAATGGTAGTTTTAAAGAAATAATTATTGCTACAAGTTATACTCATGATGGAGAAGTCACAGCTGATTATATTATAAAAATGCTTGAAGACATTAAAGACCTACAAATTTATCGTATTGGTTTTGGTATTCCTTTAAATTCAAGCATTGATTATGCCGATGATGAAACTTTAAAACAATCTCTTATCAATAAACGAAAAATAAAAATTTAA", "aa": "MIVTTNEDLERIESANIYNGLYHITNGEISLRKNVVIEHTNIKTIKERVLNGSFKEIIIATSYTHDGEVTADYIIKMLEDIKDLQIYRIGFGIPLNSSIDYADDETLKQSLINKRKIKI", "aa_hexdigest": "625582047428de2843914744ecd41b33", "start_type": "ATG", "rbs_motif": null, "genes": [ "recR" ], "truncated": "3-prime", "pseudogene": { "start": 67897, "stop": 68499, "observations": { "mutation": [ 68418 ], "stop": [ 68418 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PR56", "query_cov": 0.9915966386554622, "subject_cov": 0.5870646766169154, "identity": 0.983, "score": 228.0, "evalue": 1.75e-74, "gene_start": 1, "gene_end": 118, "reference_start": 83, "reference_end": 200, "reference_sequence": "MSKPVEFEMLVDALKSLPGVGTKNANKWAFFLLQQDQKYIDDLIKRIKEAKTNILKCKYCANFTNKDECDICLNEYRDFTKLMIVTTNEDLERIESANIYNGLYHITNGEISLRKNVVIEHTNIKTIKERVLNGSFKEIIIATSYTHDGEVTADYIIRMLEDIKDLQIYRIGFGIPLNSSIDYADDETLKQSLINKRKIRN" }, "paralog": false, "identity": 0.985, "score": 393.0, "evalue": 3.97e-142, "description": "Internal stop codon at 68418. Nonsense mutation around 68418.", "psc": { "uniref90_id": "UniRef90_Q9PR56", "gene": "recR", "product": "recombination mediator RecR", "uniref50_id": "UniRef50_Q9PR56", "cog_id": "COG0353", "cog_category": "L", "kegg_orthology_id": "K06187", "go_ids": [ "GO:0003677", "GO:0006281", "GO:0006310", "GO:0046872" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PR56", "UniRef:UniRef50_Q9PR56", "COG:COG0353", "COG:L", "KEGG:K06187", "GO:0003677", "GO:0006281", "GO:0006310", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PR56", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR56" ], "product": "Recombination protein RecR" } }, "id": "NKIECMJPJD_135", "locus": "GCA000828735_00099" }, { "type": "cds", "sequence": "contig_1", "start": 68508, "stop": 68807, "strand": "-", "frame": 1, "gene": "ybaB", "product": "YbaB/EbfC family nucleoid-associated protein", "db_xrefs": [ "COG:COG0718", "COG:K", "GO:0003677", "GO:0005737", "GO:0043590", "RefSeq:WP_006688546.1", "SO:0001217", "UniParc:UPI000169E3C1", "UniRef:UniRef100_A0AAC9X6R6", "UniRef:UniRef50_Q9PR57", "UniRef:UniRef90_Q9PR57" ], "nt": "ATGGACTTTCAAAAACTTGCACAAGAATTAAAAAAAATGCAAAACACTTTAAGTAAAAAACAAAAAGAATTTGAAGAAAAAGTGTTTGATTTTGATTATAAAGGATATGTACTTGTTAAAATCAAAGGTGATTTAAATATTGAAGCAATTGAAATAAAAACAGAAATAGTGGATCCTGAAGACAAAGAAACATTACAAGATATTTTACGTGCAGCGATCAATGAAGCAATCTCTACAACATGTAAAGAACGAGATGCGATTATGAATGCTACAATCCCTAAAGGAACAGGTCTTTTTTAG", "aa": "MDFQKLAQELKKMQNTLSKKQKEFEEKVFDFDYKGYVLVKIKGDLNIEAIEIKTEIVDPEDKETLQDILRAAINEAISTTCKERDAIMNATIPKGTGLF", "aa_hexdigest": "e39e34856f7cbbe019fb0da89b7b3cc8", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI000169E3C1", "ncbi_nrp_id": "WP_006688546.1", "uniref100_id": "UniRef100_A0AAC9X6R6", "db_xrefs": [ "SO:0001217", "UniParc:UPI000169E3C1", "RefSeq:WP_006688546.1", "UniRef:UniRef100_A0AAC9X6R6" ] }, "ips": { "uniref100_id": "UniRef100_A0AAC9X6R6", "uniref90_id": "UniRef90_Q9PR57" }, "psc": { "uniref90_id": "UniRef90_Q9PR57", "gene": "YbaB", "product": "YbaB/EbfC family nucleoid-associated protein", "uniref50_id": "UniRef50_Q9PR57", "cog_id": "COG0718", "cog_category": "K", "go_ids": [ "GO:0003677", "GO:0005737", "GO:0043590" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PR57", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR57" ], "product": "Nucleoid-associated protein UU088" }, "genes": [ "ybaB" ], "id": "NKIECMJPJD_136", "locus": "GCA000828735_00100" }, { "type": "cds", "sequence": "contig_1", "start": 69115, "stop": 69822, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCGTGAATTTATTTATGAATTATACAATCAAGGTGTTAATTTAGAAGCTTTTTGTGTGCAAATAATAAATGTTTTAATTGATTATTTAATTTATTTAAAAACAAACGATATAAATAATCTTAAAAAAGTATCAGTTGATGAATTAAAAAAAATGTTATTAATTAATTTTAATGGTAATTATTTGTTAAATAATTTTATTTCTTTATTTAATAATTTAAAAAAATCATTAAATCAAGTTTTTGAATTTGAAATTTATTTATATAAAATAATCAATGCTAATAATGATAAATTAGAAAATAAAAAAATAGAATTGTCAATTAACCCTGAAGAAAATAAAATTAATAATTCCCAAATAATAACGACAAAAAAAATCGATTGTTTTGAAGAAATGAATAAAAATACTGTTGATTTTGATAAGTTATACAAAACAAAAATTTTTTATCACAAAAAAATTAATAACCAGAATGATAAAGAATTAGGTATTAATCAAAAAGAAGAATTAGGTACAAAATTAGTTAATGATAATAAATTGATTTGCAATTCTAATGATATATTAGATAATTATGAACTAGCCAAACAAGCTTTTTTTAATAAAGACATTAAACTTTCAAAAGAAATGAGTCAAAAATTTAAAGATTTTAAAAATGAAGAAACCATTGAAAATAGTTATATTGATATTATTAAACAAGCTGATTTAGTACTTTGA", "aa": "MREFIYELYNQGVNLEAFCVQIINVLIDYLIYLKTNDINNLKKVSVDELKKMLLINFNGNYLLNNFISLFNNLKKSLNQVFEFEIYLYKIINANNDKLENKKIELSINPEENKINNSQIITTKKIDCFEEMNKNTVDFDKLYKTKIFYHKKINNQNDKELGINQKEELGTKLVNDNKLICNSNDILDNYELAKQAFFNKDIKLSKEMSQKFKDFKNEETIENSYIDIIKQADLVL", "aa_hexdigest": "594e4988b05dfbbade92c54074f0ff0a", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 27846.67470000004, "isoelectric_point": 5.70125789642334 }, "id": "NKIECMJPJD_137", "locus": "GCA000828735_00101" }, { "type": "cds", "sequence": "contig_1", "start": 70201, "stop": 70608, "strand": "-", "frame": 3, "gene": null, "product": "DNA polymerase III subunit gamma/tau", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A353KRR9" ], "nt": "ATGAGCGAATCAACATTGTACCGTAAATATCGGCCACATAAATTTAAAAATGTAATAGGTCAAGATTTAATTAAACAAGCATTAATGAATGCCTGTTTAAATAACAAAATTACACATGCTTATATTTTTAGTGGACCACGAGGAATTGGAAAAACATCAATAGCTAGAATTTTTGCGTCTAGTATTAATTGTTTAAATCCTAATAAAGGTGATTGTTGCGGCAAATGTAGTGTTTGTGAAAGTATTCTCAAAGAACAAAGTGTTGATTTGATTGAATTAGATGCTGCTAGTAATAATGGTGTTGATCAAATACGTATTATTACTGATAATATAAATTATTTACCAACATTTTTTAAGTATAAAGTCTATATTATTGATGAAGCACATATGCTATCTACATCAGCATGA", "aa": "MSESTLYRKYRPHKFKNVIGQDLIKQALMNACLNNKITHAYIFSGPRGIGKTSIARIFASSINCLNPNKGDCCGKCSVCESILKEQSVDLIELDAASNNGVDQIRIITDNINYLPTFFKYKVYIIDEAHMLSTSA", "aa_hexdigest": "e56702bb6b382239b5095571ed3a3469", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI003266BD28", "query_cov": 1.0, "subject_cov": 0.8653846153846154, "identity": 0.5479999999999999, "score": 153.0, "evalue": 2.53e-45, "valid": false, "gene": "dnaX", "product": "DNA polymerase III subunit gamma/tau", "ec_ids": [ "2.7.7.7" ], "uniref50_id": "UniRef50_A0A353KRR9", "kegg_orthology_id": "K02343", "go_ids": [ "GO:0003887", "GO:0005524", "GO:0006260", "GO:0009360" ] }, "pscc": { "uniref50_id": "UniRef50_A0A353KRR9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A353KRR9" ], "product": "DNA polymerase III subunit gamma/tau" }, "genes": [], "id": "NKIECMJPJD_138", "locus": "GCA000828735_00102" }, { "type": "cds", "sequence": "contig_1", "start": 70806, "stop": 71138, "strand": "+", "frame": 3, "gene": "dck", "product": "Deoxyguanosine kinase", "db_xrefs": [ "COG:COG1428", "COG:F", "SO:0001217", "UniRef:UniRef50_P75396", "UniRef:UniRef90_Q9PR59" ], "nt": "ATGTCAGATAAAAAATTAGATTTTTTAACACCATCTTTTAAAAAAAATAGGGTTTCTAATTCTATTGCAATAGGTGGTATGATTGCTTTTGGTAAATCTACTTTAGCACAAGCTTTACACGAGAAATATCAACCATCAAATGTGGTTTATGAAATGGTTGAAGGTGATAAATTAATGGATTTATTACTAGCAAAAATGTATGAACGTGAAAATAATGTTCTTTTTGGGTCTTTATTTCAACTATATTTTGTTTTAAATCGTTTTGCAAACTACAAAAATAATTGTAACCGTAAAAGTTTAACAATTTTTGATCGTAGTATTTTTGAAGATTGA", "aa": "MSDKKLDFLTPSFKKNRVSNSIAIGGMIAFGKSTLAQALHEKYQPSNVVYEMVEGDKLMDLLLAKMYERENNVLFGSLFQLYFVLNRFANYKNNCNRKSLTIFDRSIFED", "aa_hexdigest": "b0a50e25ef785d87d35cdeeaf072912e", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "dck" ], "truncated": "3-prime", "pseudogene": { "start": 70806, "stop": 71441, "observations": { "mutation": [ 71136, 71196, 71268 ], "stop": [ 71136, 71196, 71268 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PR59", "query_cov": 1.0, "subject_cov": 0.47413793103448276, "identity": 0.973, "score": 217.0, "evalue": 5.97e-70, "gene_start": 1, "gene_end": 110, "reference_start": 1, "reference_end": 110, "reference_sequence": "MSDKKLDFLTPSFKKNRISNSIAIGGMIAFGKSTLAQALHEKYQPSNVVYEMVEGDKLMDLLLAKMYERENNVLFGSLFQLYFVLNRFANYKNNCNRENLTIFDRSIFEDWLFAHANIDKPSVFSYYDGLWQGVCKELIYEHGVPKLYVILDGDWELFKERIFKRNRKVEIDNFSINETYFKRLLEMYKNYMVNVCKDFGIDYIVLDARNSVEHNVNEVTKRLQELKNEQNK" }, "paralog": false, "identity": 0.957345971563981, "score": 409.0, "evalue": 6.97e-148, "description": "Internal stop codon at 71136, 71196, 71268. Nonsense mutation around 71136, 71196, 71268.", "psc": { "uniref90_id": "UniRef90_Q9PR59", "gene": "Dck", "product": "Deoxyguanosine kinase", "uniref50_id": "UniRef50_P75396", "cog_id": "COG1428", "cog_category": "F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PR59", "UniRef:UniRef50_P75396", "COG:COG1428", "COG:F" ] }, "pscc": { "uniref50_id": "UniRef50_P75396", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75396" ], "product": "Uncharacterized protein MG268 homolog" } }, "id": "NKIECMJPJD_139", "locus": "GCA000828735_00103" }, { "type": "cds", "sequence": "contig_1", "start": 71364, "stop": 71504, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTACAAAAATTATATGGTTAATGTTTGTAAAGATTTTGGTATTAACTATGTTGTTTTAGATGCAAACAATTCTGTTGAACAAAACGTTGATGAAGTTACAAAAATATTACAAAAGTTAGAAAATGAGCAAGACAAATAA", "aa": "MYKNYMVNVCKDFGINYVVLDANNSVEQNVDEVTKILQKLENEQDK", "aa_hexdigest": "b4fb615d9d1f8c70e7e037b31f12aefc", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5397.0319, "isoelectric_point": 4.507070732116699 }, "id": "NKIECMJPJD_140", "locus": "GCA000828735_00104" }, { "type": "cds", "sequence": "contig_1", "start": 71703, "stop": 73124, "strand": "-", "frame": 1, "gene": "rny", "product": "Ribonuclease Y", "db_xrefs": [ "COG:COG1418", "COG:JR", "EC:3.1.-.-", "GO:0003723", "GO:0004521", "GO:0005886", "GO:0006402", "RefSeq:WP_006688749.1", "SO:0001217", "UniParc:UPI0000139226", "UniRef:UniRef100_Q9PR60", "UniRef:UniRef50_Q9PR60", "UniRef:UniRef90_Q9PR60" ], "nt": "ATGGGTTATTTAATAGCATTTATTATATTACTAATATTATTTGTATTGCTGATTACCATTGTTCCAGTTGTAATGGTAGTTTATCTTAAGAAAAAACAACTGAAATTAACTTTTGTTCCAAAAAGCCAAACTTCTTTTAAAAAAATAGTTCAAAAAACTAAAGATTTAGAAGAAGAATGTGAAGATTTAAATAATAAAAATAATGAATTAAAAAAAGCAATTAGTGATCAAAATTTACAAATTGATTTACTCAAAAAAAACAATGAAAATTTTTTACTAAATGCCACAAGTTTAACAGCTGAACAAGCGAAAAAAGAACTTTTTAATTTATTAAAAATAAAATTTAAAAAAGAGCTTGCTCAAGAATATGCAAAAATTAAACACGAATTTAATGAAGCCCAAGAAATTTATGCTCAAAACATTCTAGTAGAAACAATGGAGCAAATTGCTGAGCCTTTAATTGTTGAAAGATCTTTATTTAATATTGACATTATTGATGAAAATCTAAAAGGAAAAATTATTGGACGTGATGGACGTAATAAAGCAGTTTTTGAAAATGAAGGCGGAGTTGATCTAATTGTTGATCGACAACAGCCAATTGTAGGAATATCTACTCCAAATCCAATTCGTCGTGAAATAGCACGAATTGTTATGCAAAAATTAATTGATTCAAAAAATATTGATATTAATCGTATTGAATTACTTTTTAAAGAAGAACGTGAAAAGTTTGAAAAAAAAGTTTTTGAGATTGGTAAAAATGTCGTAGAACAAACATTAGGTTTTTTTGATTTACCTGAAGGAATTTATTCTTATATAGGGAGAATGAAATTTCGTAATTCTTATGGTCAAAATATTTTATCCCATTCATTAGAAGTTGCTGAATATGCTGAACGAATTGCTAAATTAATTAATATTGATCCTATAAAAGCAAAAAAAGCTGCATTTTTTCATGATATAGGAAAAACGATTGATTTTGAAAGCGACCTAGATCATGTAGAAGCGGGATTATTGATCGCTAAGAAATTTAATTTAGATGATTATATATATAATGCAATTGAATCACATCATAACAAAGTAATTCCAACAACAATTTATGGTGCATTAGTTAAAATAGTTGATACACTATCAGCGGCCAGACCAGGAGCACGTGTTAATTCTTATGATGAATATTACAGTCGTGTAAAAGAACTTGAAACAATATGTATGCGATTCGAAGGTGTTAAAAGCGCCTATGTCATTAAATCTGGTCGTCAATTACGTGTAATTGTTGATTCTAACTTGGTAAGTGATGAGCAATTAGAACTCTTAGGTCACGAGATCAAAGTTGCAATTGAAGAAAATGATTTATTAACAAATTATAAAATTAAGATCGTTTTAATTAAAGAAAAAAGGATTTCAATTGACACAAATATTATTGGATAG", "aa": "MGYLIAFIILLILFVLLITIVPVVMVVYLKKKQLKLTFVPKSQTSFKKIVQKTKDLEEECEDLNNKNNELKKAISDQNLQIDLLKKNNENFLLNATSLTAEQAKKELFNLLKIKFKKELAQEYAKIKHEFNEAQEIYAQNILVETMEQIAEPLIVERSLFNIDIIDENLKGKIIGRDGRNKAVFENEGGVDLIVDRQQPIVGISTPNPIRREIARIVMQKLIDSKNIDINRIELLFKEEREKFEKKVFEIGKNVVEQTLGFFDLPEGIYSYIGRMKFRNSYGQNILSHSLEVAEYAERIAKLINIDPIKAKKAAFFHDIGKTIDFESDLDHVEAGLLIAKKFNLDDYIYNAIESHHNKVIPTTIYGALVKIVDTLSAARPGARVNSYDEYYSRVKELETICMRFEGVKSAYVIKSGRQLRVIVDSNLVSDEQLELLGHEIKVAIEENDLLTNYKIKIVLIKEKRISIDTNIIG", "aa_hexdigest": "a5b48f3042b4600528c12e293211b979", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000139226", "ncbi_nrp_id": "WP_006688749.1", "uniref100_id": "UniRef100_Q9PR60", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139226", "RefSeq:WP_006688749.1", "UniRef:UniRef100_Q9PR60" ] }, "ips": { "uniref100_id": "UniRef100_Q9PR60", "uniref90_id": "UniRef90_Q9PR60" }, "psc": { "uniref90_id": "UniRef90_Q9PR60", "gene": "rny", "product": "Ribonuclease Y", "ec_ids": [ "3.1.-.-" ], "uniref50_id": "UniRef50_Q9PR60", "cog_id": "COG1418", "cog_category": "JR", "go_ids": [ "GO:0005886", "GO:0003723", "GO:0004521", "GO:0006402" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PR60", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR60" ], "product": "Ribonuclease Y" }, "genes": [ "rny" ], "id": "NKIECMJPJD_141", "locus": "GCA000828735_00105" }, { "type": "cds", "sequence": "contig_1", "start": 73294, "stop": 74160, "strand": "-", "frame": 3, "gene": "recA", "product": "recombinase RecA", "db_xrefs": [ "COG:COG0468", "COG:L", "GO:0003677", "GO:0003684", "GO:0003697", "GO:0005524", "GO:0005829", "GO:0006281", "GO:0006310", "GO:0008094", "GO:0009432", "GO:0016887", "GO:0140664", "KEGG:K03553", "SO:0001217", "UniRef:UniRef50_Q9PR61", "UniRef:UniRef90_Q9PR61" ], "nt": "ATGAAAGAAAATAATAATCTTGAGAAATTAGATCCAATTGCAACACTTGAAACAAAATTTGCTAAATCATCTTATTTTATTGCTGATGAAATAAAAGATGAAAAAATTAATGCAATATCTACAGGATCAATTCATATAGATCAAATAACAGGAATTAATGGAATTCCAGTAGGAAAAATTACAGAAATTTATGGTAATGAATCATCAGGTAAAACTACAATTGCTTTACAAACAATTGCTGAATGTCAAAAAACTGGTGGGACAGTAGTTTTGTTAGATCTTGAGGGCTCATTTGATATTAATTATGCCAAAAGTCTAAAAGTCGATTTAACAAAATTAATCATTACGCAACCTCAAACAGGTGAACAGGCTTTTGATATGATTGAAACATTGATTAAAACAAATTCAATAGATTTAATAGTTATTGATTCAGTTGCTGCAATGCTCCCAGAAAGCGAATATCAAGCAAATATGAATGAAGCATTGATGGGAGCACACGCTCGCTTAATGTCAAAAGGTTTACGTAAAATTCAACCATTAATTAATAAAAGTAAAACAGCCATTATTTTTATTAATCAATTACGTGAAAAAATTAATACTTTTTTTGGCAATCCTGAAATGACCACAGGCGGTAAAGCTTTAAAATTTTATGCTAGTTTGAGAATAGAAACCAAAAAAGCAGATTTAATTAAAGAGGGTATTAATAAAATTGGTATAAAAACAAAAGTAACAACAGTTAAAAACAAATTAGCACCACCGTTACAAACATGTTTTATTGATATTTTTTTTGGTTCTGGTTTCGATTATAATAATGAAATTATAGATTTTGCAATCCAATATGGCGTCTTGAAAAAAAACGGTTCTTGA", "aa": "MKENNNLEKLDPIATLETKFAKSSYFIADEIKDEKINAISTGSIHIDQITGINGIPVGKITEIYGNESSGKTTIALQTIAECQKTGGTVVLLDLEGSFDINYAKSLKVDLTKLIITQPQTGEQAFDMIETLIKTNSIDLIVIDSVAAMLPESEYQANMNEALMGAHARLMSKGLRKIQPLINKSKTAIIFINQLREKINTFFGNPEMTTGGKALKFYASLRIETKKADLIKEGINKIGIKTKVTTVKNKLAPPLQTCFIDIFFGSGFDYNNEIIDFAIQYGVLKKNGS", "aa_hexdigest": "5ed91fc98f26812405d289bf97114e77", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_Q9PR61", "query_cov": 1.0, "subject_cov": 0.8622754491017964, "identity": 1.0, "score": 556.0, "evalue": 2.13e-199, "valid": true, "gene": "recA", "product": "recombinase RecA", "uniref50_id": "UniRef50_Q9PR61", "cog_id": "COG0468", "cog_category": "L", "kegg_orthology_id": "K03553", "go_ids": [ "GO:0003677", "GO:0003684", "GO:0003697", "GO:0005524", "GO:0005829", "GO:0006281", "GO:0006310", "GO:0008094", "GO:0009432", "GO:0016887", "GO:0140664" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PR61", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR61" ], "product": "Protein RecA" }, "genes": [ "recA" ], "id": "NKIECMJPJD_142", "locus": "GCA000828735_00106" }, { "type": "cds", "sequence": "contig_1", "start": 74238, "stop": 74669, "strand": "-", "frame": 1, "gene": "pncC", "product": "CinA family protein", "db_xrefs": [ "COG:COG1546", "COG:H", "EC:3.5.1.42", "KEGG:K03743", "SO:0001217", "UniRef:UniRef50_A0A084EWD4", "UniRef:UniRef90_A0AAC9T390" ], "nt": "ATGAATGTGGCTTTAGAACTAATTAATTTACTAAAAGAAAGACGTTTAAGATTGAGTGTTTGCGAATCAGCAAGTTGTGGAGCTTTATCATCATCAATTGGTGAAGTTGCAGGTGCTTCAAGTGTTTTTGCAGGTGGTTTTATTAGTTATAGTAATGAAGTAAAAATTAAATTAGTAGGAGTGAGTGAAAAAACAATTTTTAAATATGGCGCTGTTTCTGAACAAACTGCTAAAGAAATGTGTTTACAAACAAATCAAAAGTTTAATACTGATATTGCAATTTCAATTACAGGAAATGCTGGACCACAAGGAAGCGAAAATAAAGAAGTAGGTTTATTTTACATTGGAATAGCAATTAAAGATTTTGCAATTGTAAAAAAAGTCACATTAAATTCAAATGAACGTACTTTTAATCGTTTTTCTATAGCATGA", "aa": "MNVALELINLLKERRLRLSVCESASCGALSSSIGEVAGASSVFAGGFISYSNEVKIKLVGVSEKTIFKYGAVSEQTAKEMCLQTNQKFNTDIAISITGNAGPQGSENKEVGLFYIGIAIKDFAIVKKVTLNSNERTFNRFSIA", "aa_hexdigest": "b2c46351efc1f6738d0c87beeb5b142d", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_A0AAC9T390", "query_cov": 1.0, "subject_cov": 0.9166666666666666, "identity": 0.937, "score": 253.0, "evalue": 1.3e-84, "valid": true, "gene": "PncC", "product": "CinA family protein", "ec_ids": [ "3.5.1.42" ], "uniref50_id": "UniRef50_A0A084EWD4", "cog_id": "COG1546", "cog_category": "H", "kegg_orthology_id": "K03743" }, "pscc": { "uniref50_id": "UniRef50_A0A084EWD4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A084EWD4" ], "product": "Competence-damage inducible protein" }, "genes": [ "cinA", "pncC" ], "id": "NKIECMJPJD_143", "locus": "GCA000828735_00107" }, { "type": "cds", "sequence": "contig_1", "start": 74777, "stop": 76975, "strand": "-", "frame": 2, "gene": "gyrA", "product": "DNA topoisomerase (ATP-hydrolyzing) subunit A", "db_xrefs": [ "COG:COG0188", "COG:L", "EC:5.6.2.2", "GO:0003677", "GO:0003916", "GO:0003918", "GO:0005524", "GO:0005694", "GO:0005737", "GO:0006261", "GO:0006265", "GO:0009330", "GO:0034335", "KEGG:K02469", "SO:0001217", "UniRef:UniRef50_P47719", "UniRef:UniRef90_Q9PR63" ], "nt": "ATGGCACAAGACTTTTCAATGCGTTATTTATTAGTTGATGGTCATGGTAATTTTGGTTCGATTGATGGTGATAGTGCAGCTGCGATGCGTTATACAGAAGCACGATTATCAAAAATCTCTTATGAATTATTAAAATACATTGATAAAGAAACAGTAGATTTTGTACCTAATTATGATGCATCAGAACAAGAACCAAGTGTTTTACCATCAGGTTTTCCAAATTTATTAACAAATGGAACTACAGGAATAGCTGTTGGGATGGCAACAAATATTCCACCACATAATTTAACTGAAGTTTGTCAAGCAATAAAAGCTTATGCTAAAAACCATAATGTTACTATTTCTGAAATTATGGAATATCTAAAAGGTCCTGATTTTCCAACAGGCGCTGAAATTTATGGTGATAGCGGTATTATTAAATATTTTAATACAGGTCGTGGTTCAGTAACAATTCGTTCAAAATATGAAATTGAAGATATTGGGCAAGGACGTGTAGCAATTGTTGTGACAGAAATTCCTTATATGGTTAATAAAGCTAATTTAATTGAAAAAATTGTTGAATTAGTAACAAACAAACAAATCGAAGGAATTTCTGATTTACGTGACGAGTCAAGTCGTGATGGAATTCGAATTGTTATTGAAGTTAAACGTGATGTTATACCTGAAGTTTTATTAAACAAATTATTTAAAACAACACCTTTACAAACAAATTTTAGTGTTAATAATTTAGCTTTAGTTAATGGTGTACCAATGGTATTAAACATTAAGGAAATGATTAAATACTATTTTGAACACCAAATTGAGATTTTAGTAAGAAGAACAAATTTTGATTTAAAAAAAGCAAAAGAACGAATTCATATTGTTGAAGGTTTAGTAATTGCTGTTAATAATATCGATGAAGTAATTAAAATTATTAAAGCCTCAGGTGATGATGATATTGCTTCAAAATCACTAATTCAACGTTTCGATTTAACAGAATTGCAAACGAAAGCAATTTTAGAAATGCGTTTACGCGCTTTAACAGGACTAAATATTGATAAATTAAAAAAAGAATATGAAGATTTAATATTAGTTATTAAAGATTTAGAAGATGTTTTAAACAACTACAATCGTCAAGTTAATATTATTTGTGAAAATTTAGATTATTTAATTGAAAAATTTGGTGATGAGCGTCGTACAGAAATTATGTATGGAGTTAGTTCGCATATTGATGATGAAGATTTAATACCAGTCGAAGATATTGTTGTAACAATGTCAAAACGTGGTTATTTTAAACGTTTGCCAATTGATACATATAAAAATCAACGACGTGGTGGTGTAGGTGTACAAGGATTAAAAACGTATGAGGATGATGATGTTGAAAAAATCTTAGTTGCTAATACCCATACAGATTTATTATTCTTTTCTGATTTAGGTCGTGTTTATCGATTACGAGGACATGAAGTACCATTGGGATCACGACAATCAAAAGGAATTCCAGCAATTAATTTTTTACCAATTGAAAAAAGTGAAAGCATTTTAACTATTTTACCAATAGATAATTATGATCAAGGTTCATTATTCTTTACAACAAGTAAAGGAATTATAAAACGAGCTAATCTAAGTGATTTCGAGTCAATTCGTGCTAATGGTAAAATTGCTATTACTTTAAAAGATGGTGATAAATTATTTTCAGTAATGCAAACACTAGGTAATGATGAAGTATTTATCGGCGCATCAAATGGTAATGTAATTCGTTTCAATGAAAATGATGCACGTGAAATGGGAAGAATTGCGACAGGTGTTAAAGGAATTAATTTAGAAAATGATGAATATGTTGTAGGAACAGGGTTATCTAGTCATGGAGAATATGTTTTAGCTGTTGGTTCTAAAGGATTAGGTAAATTAACTGACATCAATGATTATCGTTTAACTAAGCGTGGAGCAAAAGGTGTTAATACACTAAAAGTAAATGATAAAACAGGTAACTTAGTAAGTATTAAAGTTGTTAATCGTGAAGAAGAAGCTTTAATTATTACAACTAGTGGAAAAGTTATTCGTTTATCGATTAAAGACATTAGTGTAATTGGAAGAAATACATCAGGAGTTAAATTAATTTCATTAGAAAATAAAGAAGAAGTAAAATCAATTGCTATCTTTAAAAAAGAAGAAATTAATGATGAACAATTATTACAAGAAAATGATTACAATCTAAAATAA", "aa": "MAQDFSMRYLLVDGHGNFGSIDGDSAAAMRYTEARLSKISYELLKYIDKETVDFVPNYDASEQEPSVLPSGFPNLLTNGTTGIAVGMATNIPPHNLTEVCQAIKAYAKNHNVTISEIMEYLKGPDFPTGAEIYGDSGIIKYFNTGRGSVTIRSKYEIEDIGQGRVAIVVTEIPYMVNKANLIEKIVELVTNKQIEGISDLRDESSRDGIRIVIEVKRDVIPEVLLNKLFKTTPLQTNFSVNNLALVNGVPMVLNIKEMIKYYFEHQIEILVRRTNFDLKKAKERIHIVEGLVIAVNNIDEVIKIIKASGDDDIASKSLIQRFDLTELQTKAILEMRLRALTGLNIDKLKKEYEDLILVIKDLEDVLNNYNRQVNIICENLDYLIEKFGDERRTEIMYGVSSHIDDEDLIPVEDIVVTMSKRGYFKRLPIDTYKNQRRGGVGVQGLKTYEDDDVEKILVANTHTDLLFFSDLGRVYRLRGHEVPLGSRQSKGIPAINFLPIEKSESILTILPIDNYDQGSLFFTTSKGIIKRANLSDFESIRANGKIAITLKDGDKLFSVMQTLGNDEVFIGASNGNVIRFNENDAREMGRIATGVKGINLENDEYVVGTGLSSHGEYVLAVGSKGLGKLTDINDYRLTKRGAKGVNTLKVNDKTGNLVSIKVVNREEEALIITTSGKVIRLSIKDISVIGRNTSGVKLISLENKEEVKSIAIFKKEEINDEQLLQENDYNLK", "aa_hexdigest": "ddb475e8438ace3b9658b83d13a196b0", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_Q9PR63", "query_cov": 1.0, "subject_cov": 0.8611764705882353, "identity": 1.0, "score": 1401.0, "evalue": 0.0, "valid": true, "gene": "gyrA", "product": "DNA topoisomerase (ATP-hydrolyzing) subunit A", "ec_ids": [ "5.6.2.2" ], "uniref50_id": "UniRef50_P47719", "cog_id": "COG0188", "cog_category": "L", "kegg_orthology_id": "K02469", "go_ids": [ "GO:0003677", "GO:0003916", "GO:0003918", "GO:0005524", "GO:0005694", "GO:0005737", "GO:0006261", "GO:0006265", "GO:0009330", "GO:0034335" ] }, "pscc": { "uniref50_id": "UniRef50_P47719", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47719" ], "product": "DNA gyrase subunit A" }, "genes": [ "gyrA" ], "id": "NKIECMJPJD_144", "locus": "GCA000828735_00108" }, { "type": "cds", "sequence": "contig_1", "start": 77042, "stop": 77299, "strand": "-", "frame": 2, "gene": null, "product": "DNA topoisomerase type IIA domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A960F5Y9" ], "nt": "ATGGCATTAAAAAAACCAAAAAAAAGTCGTTTAACCACCGAAGAAATTAAGCAACAATTAGAAGGTTCAACGATTAAAGAACAATCAATTACAAAAGAAGTCGAAACATCATTTTTAGATTATTCAATGTCTGTAATCGTTGCACGTGCTTTGCCTGATGTGCGTGATGGATTTAAGCCCGTGCATCGCCGAGCTTTATTTGCTGCTTTCGAAAACGGAATGACACATGATAAGCCTTATAAAAAATCTGCACGTTGA", "aa": "MALKKPKKSRLTTEEIKQQLEGSTIKEQSITKEVETSFLDYSMSVIVARALPDVRDGFKPVHRRALFAAFENGMTHDKPYKKSAR", "aa_hexdigest": "7d697c8fafb414dda9228e5a0310968d", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "psc": { "uniref90_id": "UniRef90_A0A920J4M1", "query_cov": 0.8941176470588236, "subject_cov": 0.8539325842696629, "identity": 0.5, "score": 69.3, "evalue": 9.82e-14, "valid": false, "product": "DNA topoisomerase type IIA domain-containing protein", "uniref50_id": "UniRef50_A0A960F5Y9" }, "pscc": { "uniref50_id": "UniRef50_A0A960F5Y9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A960F5Y9" ], "product": "DNA topoisomerase type IIA domain-containing protein" }, "genes": [], "id": "NKIECMJPJD_145", "locus": "GCA000828735_00109" }, { "type": "cds", "sequence": "contig_1", "start": 77321, "stop": 77473, "strand": "-", "frame": 2, "gene": null, "product": "DNA topoisomerase (ATP-hydrolyzing)", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2H6AWV8" ], "nt": "ATGGATCCTAAAAATAGACTTTTATTAAAAGTTAATATTGAAGACGCTGCAATTGCTGATAAAACATTTTCTCTATTAATGGGTGATGATGTAGCTCCAAGAAAAGAATTTATTGAAAAAAATGCAAAATATGTAAAAAATATTGATGCTTAA", "aa": "MDPKNRLLLKVNIEDAAIADKTFSLLMGDDVAPRKEFIEKNAKYVKNIDA", "aa_hexdigest": "4d2f0a52842685f0712bbbc265b4eff8", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_A0A538DNN0", "query_cov": 0.98, "subject_cov": 0.8166666666666667, "identity": 0.633, "score": 67.8, "evalue": 4.74e-14, "valid": false, "product": "DNA topoisomerase (ATP-hydrolyzing)", "uniref50_id": "UniRef50_A0A2H6AWV8" }, "pscc": { "uniref50_id": "UniRef50_A0A2H6AWV8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2H6AWV8" ], "product": "DNA topoisomerase (ATP-hydrolyzing)" }, "genes": [], "id": "NKIECMJPJD_146", "locus": "GCA000828735_00110" }, { "type": "cds", "sequence": "contig_1", "start": 77483, "stop": 78097, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAGCTGCTGAAGCACGACGACGTTCACATGAAGCACGTGAAGCTACACGGCGTAAATCACCTTTTGAATCAAATTCATTACCTGGTAAATTAGCTGACTGCTCAAATCGTGATTCAAGTGTTACTGAAATTTATATTGTCGAAGGAGATTCAGCGGGTGGTTCAGCAAAAACAGGTCGTGAGCGTGAATTTCAAGCAATTTTACCACTGCGTGGAAAGATTATTAATGTTGAAAAAGCTAAAATTGATAAGATTTTTGCTAATGAAGAAATTCAAAATATGATTACTGCTTTTGGAGCAGGAATAGGTCCTGAATTTAATATTGAAAAATTAAGATATTCAAAAATTATTATTATGACTGATGCGGATGTTGATGGCAGTCATATTCGAATTCTATTGCTAACATTTTTTTATCGATACATGTTGCCATTGATTCAAAATGGAAATGTTTATATAGCGCAACCACCATTGTATAAAGTAAGTTATGCTAAAACAATTAAATACGCATATTCTGATCAAGAGTTAGAAAGAATTAAATCAACAATATCAAATATTAAATATAACATTCAACGCTATAAAGGGTTAGGTGAAATGAATCCTGATCAATTGTGA", "aa": "MQAAEARRRSHEAREATRRKSPFESNSLPGKLADCSNRDSSVTEIYIVEGDSAGGSAKTGREREFQAILPLRGKIINVEKAKIDKIFANEEIQNMITAFGAGIGPEFNIEKLRYSKIIIMTDADVDGSHIRILLLTFFYRYMLPLIQNGNVYIAQPPLYKVSYAKTIKYAYSDQELERIKSTISNIKYNIQRYKGLGEMNPDQL", "aa_hexdigest": "414da027a88c3c33768eb728a9326c3c", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0028ACB8D2", "query_cov": 0.9509803921568627, "subject_cov": 0.9326923076923077, "identity": 0.593, "score": 224.0, "evalue": 2.94e-71, "valid": false, "product": "toprim domain-containing protein", "uniref50_id": "UniRef50_A0A7L7RZ60" }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 23147.202100000013, "isoelectric_point": 9.175349998474118 }, "id": "NKIECMJPJD_147", "locus": "GCA000828735_00111" }, { "type": "cds", "sequence": "contig_1", "start": 78863, "stop": 79108, "strand": "-", "frame": 2, "gene": null, "product": "DNA topoisomerase (ATP-hydrolyzing)", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A380EBM1" ], "nt": "ATGGGCGGATTTGCAACAATTGTTAGAGTTATTCTAAAAAAAGACGGATCAGTACGTGTAGAAGATGATGGACGCGGAATTCCAATTGAAATTCACGAAAAAACAGGCTTATCAGGTGTTGAAACAGTATTAACAGTTTTACATGCTGGTGGTAAGTTTGATAATGATAGTTATAAAGTGTCTGGTGGATTACATGGTGTTGGTGCCTCTGTTGTTAATGCTTTAAGTAAAAATTTTAAGGTTTGA", "aa": "MGGFATIVRVILKKDGSVRVEDDGRGIPIEIHEKTGLSGVETVLTVLHAGGKFDNDSYKVSGGLHGVGASVVNALSKNFKV", "aa_hexdigest": "cd0671b3ab80aeb79da0d37027158844", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0021B43CD8", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.568, "score": 99.4, "evalue": 9.24e-26, "valid": false, "product": "ATP-binding protein", "uniref50_id": "UniRef50_A0A380EBM1" }, "pscc": { "uniref50_id": "UniRef50_A0A380EBM1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A380EBM1" ], "product": "DNA topoisomerase (ATP-hydrolyzing)" }, "genes": [], "id": "NKIECMJPJD_148", "locus": "GCA000828735_00112" }, { "type": "cds", "sequence": "contig_1", "start": 79139, "stop": 79273, "strand": "-", "frame": 2, "gene": null, "product": "DNA topoisomerase (ATP-hydrolyzing)", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A920FT50" ], "nt": "ATGAACGATTCTAATAAAGAAAAAAAATACACCGCCGAAAGTATTAAAGTACTAGAAGGTTTAGAAGCAGTACGAAAACGTCCTGGTATGTACATTGGTTCAACCCAATCAGAGGGTCTACACCATATGATTTGA", "aa": "MNDSNKEKKYTAESIKVLEGLEAVRKRPGMYIGSTQSEGLHHMI", "aa_hexdigest": "a93637b41d64556bdddaeb83f0405c74", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_A0A920FT50", "query_cov": 0.9090909090909091, "subject_cov": 0.8, "identity": 0.6, "score": 49.7, "evalue": 4.33e-07, "valid": false, "product": "DNA topoisomerase (ATP-hydrolyzing)", "uniref50_id": "UniRef50_A0A920FT50" }, "pscc": { "uniref50_id": "UniRef50_A0A920FT50", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A920FT50" ], "product": "DNA topoisomerase (ATP-hydrolyzing)" }, "genes": [], "id": "NKIECMJPJD_149", "locus": "GCA000828735_00113" }, { "type": "cds", "sequence": "contig_1", "start": 79285, "stop": 79389, "strand": "-", "frame": 3, "gene": "ybcJ", "product": "RNA-binding protein", "db_xrefs": [ "COG:COG2501", "COG:J", "KEGG:K14761", "SO:0001217", "UniRef:UniRef50_A0AAP9AC47", "UniRef:UniRef90_A0AAC9T049" ], "nt": "ATGGTTGATGAAAAAATAGAAAATAGACGTGGTCGTAAATTATATGATCAAATGATTATTAAAATAAAAAATCAATTGTATCAAATAGTTAAAACTAATGAGTAG", "aa": "MVDEKIENRRGRKLYDQMIIKIKNQLYQIVKTNE", "aa_hexdigest": "53bf703dacef26a493c3c118970f67a1", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "ybcJ" ], "truncated": "3-prime", "pseudogene": { "start": 79285, "stop": 79503, "observations": { "mutation": [ 79410 ], "stop": [ 79410 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAC9T049", "query_cov": 1.0, "subject_cov": 0.4722222222222222, "identity": 1.0, "score": 67.4, "evalue": 4.51e-14, "gene_start": 1, "gene_end": 34, "reference_start": 39, "reference_end": 72, "reference_sequence": "MKKIFISTEYVTLNQFLKIAGLINNGGQAKFWLLENKVMVDEKIENRRGRKLYDQMIIKIKNQLYQIVKTNE" }, "paralog": false, "identity": 0.9861111111111112, "score": 139.0, "evalue": 4.58e-45, "description": "Internal stop codon at 79410. Nonsense mutation around 79410.", "psc": { "uniref90_id": "UniRef90_A0AAC9T049", "gene": "YbcJ", "product": "RNA-binding protein", "uniref50_id": "UniRef50_A0AAP9AC47", "cog_id": "COG2501", "cog_category": "J", "kegg_orthology_id": "K14761", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAC9T049", "UniRef:UniRef50_A0AAP9AC47", "COG:COG2501", "COG:J", "KEGG:K14761" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAP9AC47", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAP9AC47" ], "product": "S4 domain-containing protein YaaA" } }, "id": "NKIECMJPJD_150", "locus": "GCA000828735_00114" }, { "type": "cds", "sequence": "contig_1", "start": 79641, "stop": 80780, "strand": "-", "frame": 1, "gene": "dnaN", "product": "DNA polymerase III subunit beta", "db_xrefs": [ "COG:COG0592", "COG:L", "EC:2.7.7.7", "KEGG:K02338", "RefSeq:WP_006688589.1", "SO:0001217", "UniParc:UPI00000C1B8C", "UniRef:UniRef100_Q9PR66", "UniRef:UniRef50_B5ZAQ8", "UniRef:UniRef90_Q9PR66" ], "nt": "ATGGAAGTTTTTATTTCTATAAAAAAATTAATTGAAGCAATAAAATTTAGTACAACAATTGCTAATACAAACAATGCAAATGCTTTATTATTAGGTGTTTTGATTGAAGTTAGTCAAAATAAAATTAGTTTTAAGACGACAAATAATCAAGTGAGTGGTTATAAAGAAATTACTGAAGGATTTGAATATTTTAATAGTGGAAAAGTTTTAGTTACAGCTAAGATTATTTTAGGATTAATTTCTAAGCTTAAAGATAAATCTGTTTTATTAAAACAAATTGACACAAATATTTTATTAATTAAAACTGAAAATTTTGAAACTCAAATTAATACAATGAATATTGAAGGCTTTCCTAATTTAAACTTTAGTTTAGAAGATTACGTTAAAATCAGTTTGCCGCATCAAATTATGCAAGAGATTAATACAAAGGTTTTACCCAACGTTTTGAACAGTCAAGGAATTGAAAAAATTCAACCGATTTCTGGAATTTTGATTGATACTCAAACTTTAGAAAATAAGTTGATTGCTATTGGTACAGATAAAATTAAAGCTTCATGTTTAATAAGAGAATATATGGGTGAAAAATTTAAATTTATTGTTTCTTATTCAACAATGAAATTGATCATCGAAATCCTTAAAAATGTTGAATATAGTAATAATCAAACTGTTGATTTTTATGTTCGAAGTAGAAGTTTAATTTTTAAAATTAATGATGCAATTTTACAAACAAGAATGATTGATGGTGTTTATCCAAATATTTACTCAATTTTTGATGAAACAAATGAAGAAAATACATATGTTTTTGATCGTCGTTTATTGATAGAAATTATTGAACGAGGTATGAATATTGTTATGCAAGAACAAAACCCTAAAATTAGTATTAGAATAGACAAAAATGAAGCAGAAATCAGTTTAACAACTTTTGAAATTGGTAATATGAAAGAAAAAATGTCAATAGTTAATTTAAACAATAAAAGTGCTGAGTTTATTTTAAATCCTAGTTTGCTAGCTCATGTTTTAAAAAATTTTGATAACAACGATGTAACTTTTAAAGTTAAAGATGAAATTTTAAGACCTATTATTTTTATCGATACCAAAGATGTTGGATTTAAACAGATTTTATCAAGAATAAAAAATTAA", "aa": "MEVFISIKKLIEAIKFSTTIANTNNANALLLGVLIEVSQNKISFKTTNNQVSGYKEITEGFEYFNSGKVLVTAKIILGLISKLKDKSVLLKQIDTNILLIKTENFETQINTMNIEGFPNLNFSLEDYVKISLPHQIMQEINTKVLPNVLNSQGIEKIQPISGILIDTQTLENKLIAIGTDKIKASCLIREYMGEKFKFIVSYSTMKLIIEILKNVEYSNNQTVDFYVRSRSLIFKINDAILQTRMIDGVYPNIYSIFDETNEENTYVFDRRLLIEIIERGMNIVMQEQNPKISIRIDKNEAEISLTTFEIGNMKEKMSIVNLNNKSAEFILNPSLLAHVLKNFDNNDVTFKVKDEILRPIIFIDTKDVGFKQILSRIKN", "aa_hexdigest": "6ee0b32204ea492f46ce31320dd48e07", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "ups": { "uniparc_id": "UPI00000C1B8C", "ncbi_nrp_id": "WP_006688589.1", "uniref100_id": "UniRef100_Q9PR66", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1B8C", "RefSeq:WP_006688589.1", "UniRef:UniRef100_Q9PR66" ] }, "ips": { "uniref100_id": "UniRef100_Q9PR66", "uniref90_id": "UniRef90_Q9PR66" }, "psc": { "uniref90_id": "UniRef90_Q9PR66", "gene": "DnaN", "product": "DNA polymerase III subunit beta", "ec_ids": [ "2.7.7.7" ], "uniref50_id": "UniRef50_B5ZAQ8", "cog_id": "COG0592", "cog_category": "L", "kegg_orthology_id": "K02338" }, "pscc": { "uniref50_id": "UniRef50_B5ZAQ8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B5ZAQ8" ], "product": "DNA polymerase III, beta subunit" }, "genes": [ "dnaN" ], "id": "NKIECMJPJD_151", "locus": "GCA000828735_00115" }, { "type": "cds", "sequence": "contig_1", "start": 81002, "stop": 81562, "strand": "+", "frame": 2, "gene": "pth", "product": "aminoacyl-tRNA hydrolase", "db_xrefs": [ "COG:COG0193", "COG:J", "EC:3.1.1.29", "GO:0004045", "GO:0005737", "GO:0006412", "RefSeq:WP_006688439.1", "SO:0001217", "UniParc:UPI000013293A", "UniRef:UniRef100_Q9PR67", "UniRef:UniRef50_Q9PR67", "UniRef:UniRef90_Q9PR67" ], "nt": "GTGGAAAAATATTTAATTGTTGGATTAGGTAATCCAGGATCAAACTATGCAAAAACAAGACATAATGTTGGCTTTATGGTTATTAATGAGATTTGCAACAAACTTAATTTGTCTCTAGATAGTTCAAAATTTAATGGAATTTTTACTAAAACAATTTATAACAATTCCATAGTGTTTTTTTGTCAACCAACAACTTACATGAATTTAAGTGGTGAATTTGTTATCAAAATGCTTAATTTTTATAATATTCCAATTAAAAATTTAATAGTTATTTATGATGATGTTGATACGAAATTAGGAACGATAAAATTGAGAAAAAAGGGGTCCTCAGGAGGGCAAAATGGAATTAAAAATATTATTAACATTTTAAAAACAGATGAAATTAAACGCATTCGTATAGGAATTGATAAAGATCCACATATAAAATTAGATCAATATGTCTTATCGAATTTTAAGATTGACGAATTAGTTATAATTAAACCAGCTATTATAAAGGGCGCTTTAGCTACATTAGCAGCGATTGGTGAAGATTTTGATAAAGTTATGAACAAATTTAATTAA", "aa": "MEKYLIVGLGNPGSNYAKTRHNVGFMVINEICNKLNLSLDSSKFNGIFTKTIYNNSIVFFCQPTTYMNLSGEFVIKMLNFYNIPIKNLIVIYDDVDTKLGTIKLRKKGSSGGQNGIKNIINILKTDEIKRIRIGIDKDPHIKLDQYVLSNFKIDELVIIKPAIIKGALATLAAIGEDFDKVMNKFN", "aa_hexdigest": "cd970cf7be8030f43f0b2d0726761c61", "start_type": "GTG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI000013293A", "ncbi_nrp_id": "WP_006688439.1", "uniref100_id": "UniRef100_Q9PR67", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013293A", "RefSeq:WP_006688439.1", "UniRef:UniRef100_Q9PR67" ] }, "ips": { "uniref100_id": "UniRef100_Q9PR67", "uniref90_id": "UniRef90_Q9PR67" }, "psc": { "uniref90_id": "UniRef90_Q9PR67", "gene": "pth", "product": "aminoacyl-tRNA hydrolase", "ec_ids": [ "3.1.1.29" ], "uniref50_id": "UniRef50_Q9PR67", "cog_id": "COG0193", "cog_category": "J", "go_ids": [ "GO:0004045", "GO:0005737", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PR67", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR67" ], "product": "Peptidyl-tRNA hydrolase" }, "genes": [ "pth" ], "id": "NKIECMJPJD_152", "locus": "GCA000828735_00116" }, { "type": "cds", "sequence": "contig_1", "start": 81615, "stop": 82127, "strand": "+", "frame": 3, "gene": "tilS", "product": "tRNA(Ile)-lysidine synthase", "db_xrefs": [ "COG:COG0037", "COG:J", "EC:6.3.4.19", "GO:0005524", "GO:0005737", "GO:0006400", "GO:0032267", "KEGG:K04075", "SO:0001217", "UniRef:UniRef50_Q9PR68", "UniRef:UniRef90_Q9PR68" ], "nt": "ATGGCAATGTTAAATATGTACAAAAGAAATATTCGTGTTGTTTGTCATGTGAATTATCATAAACGCGAATCATCCGATCGTGATCAGCAAATAGTTGTCGATTTTTGTAAAAAAAATAATTTAATAATTGAAATTTTAGATGTTGATGAAAAAGTTTATGAACGTTATGGTCATATTGATAATTTTCAAACAAAAGCACGTTTAATTCGTTATGATTTTTTCAAAGAAATTGGTAAAAAATATAATATTGAAAATTTGTATATAGCGCATAATTTTGATGATTTTTTAGAAACGGCATATATGCAACGAGCTCGCCAATCAAAAGCCTTATTTTATGGCATTAAGGAAAGTAATGTTATTAATGGCATAATTGTTAAACGTCCGGTTTTATTTGTGCGTAAACAAACATTACAACGCTATTGTGATGAAAATAAAATTAAGTATGGTATTGACGAAACAAATGAATTAGATATATACGAACGCAATCGTGTTCGTAAAATAATTAGTTCTTGA", "aa": "MAMLNMYKRNIRVVCHVNYHKRESSDRDQQIVVDFCKKNNLIIEILDVDEKVYERYGHIDNFQTKARLIRYDFFKEIGKKYNIENLYIAHNFDDFLETAYMQRARQSKALFYGIKESNVINGIIVKRPVLFVRKQTLQRYCDENKIKYGIDETNELDIYERNRVRKIISS", "aa_hexdigest": "286e487d43be7e22c15b3a107228c321", "start_type": "ATG", "rbs_motif": "GGxGG", "genes": [ "tilS" ], "truncated": "both", "pseudogene": { "start": 81534, "stop": 82430, "observations": { "mutation": [ 81546, 82125 ], "stop": [ 81546, 82125 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PR68", "query_cov": 1.0, "subject_cov": 0.5074626865671642, "identity": 0.976, "score": 336.0, "evalue": 3.12e-114, "gene_start": 1, "gene_end": 170, "reference_start": 28, "reference_end": 197, "reference_sequence": "MIKLWTNLINRISNKKYLAAVSGGPDSMAMLNMYKRNIRVVCHVNYHKRESSDRDQQIVVDFCKKNNLIIEILNVDEKVYERYGHIDNFQAKARLIRYDFFKEIGKKYNIENLYTAHNFDDFLETAYMQRARQSKALFYGIRESNVINGIIVKRPVLFVRKQTLQRYCDENKIKYGIDETNELDIYERNRVRKIISSWSSNEVYDFKKFVIKYNKEHSSFANFIDLSYIEFKKNKYKYDYFIRQDDMVQYYLIYYFLIDHKIINPSENKIISLIKFFGKQINKEKAYRVQENIYMHVNDDDLISLISYNKNNNISNSNVDIIDDFNVIEKQVDNN" }, "paralog": false, "identity": 0.9731543624161074, "score": 585.0, "evalue": 5.64e-215, "description": "Internal stop codon at 81546, 82125. Nonsense mutation around 81546, 82125.", "psc": { "uniref90_id": "UniRef90_Q9PR68", "gene": "tilS", "product": "tRNA(Ile)-lysidine synthase", "ec_ids": [ "6.3.4.19" ], "uniref50_id": "UniRef50_Q9PR68", "cog_id": "COG0037", "cog_category": "J", "kegg_orthology_id": "K04075", "go_ids": [ "GO:0005737", "GO:0005524", "GO:0032267", "GO:0006400" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PR68", "EC:6.3.4.19", "UniRef:UniRef50_Q9PR68", "COG:COG0037", "COG:J", "KEGG:K04075", "GO:0005737", "GO:0005524", "GO:0032267", "GO:0006400" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PR68", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR68" ], "product": "tRNA(Ile)-lysidine synthase" } }, "id": "NKIECMJPJD_153", "locus": "GCA000828735_00117" }, { "type": "cds", "sequence": "contig_1", "start": 82272, "stop": 82526, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTCAATATTATTTAATTTACTACTTTTTAATTGATCATAAAATTATTAATCCAAGTGAAAATAAAATCATTTCATTAATCAAATTCTTTGGTAAACAAATTAATAAAGAAAAAGCTTATCGTGTTCAAGAAAATATTTATATGCATGTTAATGATGATGAATTAATTAGTTTAATATCATGCAATAAAAGCGATAATATTAGCAACTCTAATGTTGATATTATCGATGATCATAATGTTATTGAGAAATAG", "aa": "MVQYYLIYYFLIDHKIINPSENKIISLIKFFGKQINKEKAYRVQENIYMHVNDDELISLISCNKSDNISNSNVDIIDDHNVIEK", "aa_hexdigest": "733963971e1f0531627d77f54a1de737", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9967.280699999994, "isoelectric_point": 5.458157539367675 }, "id": "NKIECMJPJD_154", "locus": "GCA000828735_00118" }, { "type": "cds", "sequence": "contig_1", "start": 82541, "stop": 82699, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAATTCGTGGTAAATTATTTGTTAGTCAAGTTGTTAGAAAGTTTAATTTAAACGTTGTTGCAAATTCTGACTATATTGATCGTGAGATTTCTACAACAGGAATTACACGTGTTGGATTTGAATTAGCTGGCGAAATTTTGTTTAAAGAGATTTGA", "aa": "MEIRGKLFVSQVVRKFNLNVVANSDYIDREISTTGITRVGFELAGEILFKEI", "aa_hexdigest": "8a049a862463c58cf04c72de3b4eb0b2", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5917.787799999996, "isoelectric_point": 6.133973121643066 }, "id": "NKIECMJPJD_155", "locus": "GCA000828735_00119" }, { "type": "cds", "sequence": "contig_1", "start": 82841, "stop": 83467, "strand": "+", "frame": 2, "gene": "hprK", "product": "HPr(Ser) kinase/phosphatase", "db_xrefs": [ "COG:COG1493", "COG:T", "EC:2.7.11.-", "EC:2.7.4.-", "GO:0000155", "GO:0000160", "GO:0000287", "GO:0004674", "GO:0004712", "GO:0005524", "GO:0006109", "KEGG:K06023", "SO:0001217", "UniRef:UniRef50_Q9PR69", "UniRef:UniRef90_Q9PR69" ], "nt": "TTGTTAAAATTAGCAGAGCGCTATAAAATTCCAATTGTAGAGGTTAAATATAGTTTTTATGAATTAAATTTTACAATTAATAGTTACATTAGTCAAAAACTTTCACACCAATCATTAGTTCACGGAACATTATTATCTGTTTTTGGAATCGGCGTTATTTTAATGGGGGAATCAGGTGTTGGTAAATCTGAGTTAGCAATTGAATTAGTTAAAAAAGGTCATATTTTTGTAGGTGATGATGCTATTTTAGTTAATAGAATTGGAGGTAATCTATATGGAAGAGCTGAAGATTCGACTAAAGATTTTATTGAAATTAGAGGTTTAGGTATTATGAATTTTTCACGTTCCTTCGGTATTGAACGAATGATTGAATCAACAAAAATTGAAATAGTTATTGAATTAATAAAACCAGCTAAACATGAAAAAATCAAGTTTGAACGTTTTGGTCGTGAAATTCAACACAAAGAATTTTTAGAAACAAAAATAGCCCATTATTATATTCCGGTTATCGAAGGAAGAAGTATTTCAGATATCATTGAGACAGCCATTACTGATTATAAATTAAAAACATCAGGTTATAATTCAGCTGAAGAATTTATTCTACAAATTAATAAAAAGGGGATTTAA", "aa": "MLKLAERYKIPIVEVKYSFYELNFTINSYISQKLSHQSLVHGTLLSVFGIGVILMGESGVGKSELAIELVKKGHIFVGDDAILVNRIGGNLYGRAEDSTKDFIEIRGLGIMNFSRSFGIERMIESTKIEIVIELIKPAKHEKIKFERFGREIQHKEFLETKIAHYYIPVIEGRSISDIIETAITDYKLKTSGYNSAEEFILQINKKGI", "aa_hexdigest": "4e3cb5ccaf8df3140c15aee194c4af40", "start_type": "TTG", "rbs_motif": null, "genes": [ "hprK" ], "truncated": "5-prime", "pseudogene": { "start": 82541, "stop": 83467, "observations": { "mutation": [ 82697 ], "stop": [ 82697 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PR69", "query_cov": 1.0, "subject_cov": 0.6753246753246753, "identity": 0.995, "score": 398.0, "evalue": 1.31e-138, "gene_start": 1, "gene_end": 208, "reference_start": 101, "reference_end": 308, "reference_sequence": "MEIRGKLFVSQVVRKFNLNVVANSDYIDREISTTGITRVGFELAGEILFKEIWNIVYFGSKESNYFNKFSETIISKKLGKILDLNPPLIIFGKNFKHAGILLKLAERYKIPIVEVKYSFYELNFTINSYISQKLSHQSLVHGTLLSVFGIGVILMGESGVGKSELAIELVKKGHIFVGDDAILVNRIGGNLYGRAEDSTKDFIEIRGLGIMNFSRSFGIERMIESTKIEIVIELIKPAKHEKIKFERFGREIQHKEFLETKIAHYYIPVIEGRSISDIIETAITDYKLKTSGYNSAEEFILQINKKGI" }, "paralog": false, "identity": 0.9967532467532467, "score": 595.0, "evalue": 1e-218, "description": "Internal stop codon at 82697. Nonsense mutation around 82697.", "psc": { "uniref90_id": "UniRef90_Q9PR69", "gene": "hprK", "product": "HPr(Ser) kinase/phosphatase", "ec_ids": [ "2.7.11.-", "2.7.4.-" ], "uniref50_id": "UniRef50_Q9PR69", "cog_id": "COG1493", "cog_category": "T", "kegg_orthology_id": "K06023", "go_ids": [ "GO:0000155", "GO:0000160", "GO:0000287", "GO:0004674", "GO:0004712", "GO:0005524", "GO:0006109" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PR69", "EC:2.7.11.-", "EC:2.7.4.-", "UniRef:UniRef50_Q9PR69", "COG:COG1493", "COG:T", "KEGG:K06023", "GO:0000155", "GO:0000160", "GO:0000287", "GO:0004674", "GO:0004712", "GO:0005524", "GO:0006109" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PR69", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR69" ], "product": "HPr kinase/phosphorylase" } }, "id": "NKIECMJPJD_156", "locus": "GCA000828735_00120" }, { "type": "cds", "sequence": "contig_1", "start": 83468, "stop": 83566, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAATTAGAAATTATTAATCCAGAAAGCACATTAGTTAATGATGTTGTTGCACATCGAGTAGCTTTTAGTATTGGTAGCAATTTTAATATATATTGA", "aa": "MQLEIINPESTLVNDVVAHRVAFSIGSNFNIY", "aa_hexdigest": "beb5310543600254321f06f3ac402bf0", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3592.0397999999996, "isoelectric_point": 4.649395561218263 }, "id": "NKIECMJPJD_157", "locus": "GCA000828735_00121" }, { "type": "cds", "sequence": "contig_1", "start": 83783, "stop": 83902, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATTGCTGGGGTATTAAGTGCTGCAGTTTATTTTCCTCTTATTTTGCGCATGCCTAAATATCACATTCGAGATATTGATGCTGATGGCAATGTTGTTATTCGCCAACCTAGTATGTGA", "aa": "MIAGVLSAAVYFPLILRMPKYHIRDIDADGNVVIRQPSM", "aa_hexdigest": "56a1b836fc876ec43ec65d0842caca8d", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4372.185400000001, "isoelectric_point": 8.273501777648928 }, "id": "NKIECMJPJD_158", "locus": "GCA000828735_00122" }, { "type": "cds", "sequence": "contig_1", "start": 84032, "stop": 84475, "strand": "+", "frame": 2, "gene": "lgt", "product": "prolipoprotein diacylglyceryl transferase", "db_xrefs": [ "COG:COG0682", "COG:M", "EC:2.5.1.145", "GO:0005886", "GO:0008961", "GO:0009249", "GO:0016020", "GO:0042158", "SO:0001217", "UniRef:UniRef50_Q9PR70", "UniRef:UniRef90_Q9PR70" ], "nt": "ATGCCAGCTGTTTTCGAAGGAATGAAACATTATTTCATTGAGGGCAATAAAACTCTATTTACAATTTATCAACCTTTATTTCTATATGAATCATTCTTTAATGTAATTGTTTTTGTTTTTATTTATTTTGGCTTGTCTTACATTAAACAATTAAAAATAGGCTTTATTTCAATGTCATATTTTTTCTTTTATGGTGTTACTCGTTTTAGTACGGAGTCAGCTCGTGCTCCACAGTTTAGTTTTGAAGGAACTTATATAATTAATTCATTGTTATTAATCTTTGGTGTATTAGGTGCATTATATGTACAATTTATAGCGCCACTTTTAAGAAAAAAATTCTTATTAGATGCAATTATTGAAATGTTTTATAAAAAGAAAGACCAAATTCATAAATTTGGAGAATTAAGAAAGCCAGAAGAATTTTTATTTTATTGTCATAAATAA", "aa": "MPAVFEGMKHYFIEGNKTLFTIYQPLFLYESFFNVIVFVFIYFGLSYIKQLKIGFISMSYFFFYGVTRFSTESARAPQFSFEGTYIINSLLLIFGVLGALYVQFIAPLLRKKFLLDAIIEMFYKKKDQIHKFGELRKPEEFLFYCHK", "aa_hexdigest": "e0337b677e5a5b79cbb2afb73a6b656a", "start_type": "ATG", "rbs_motif": null, "genes": [ "lgt" ], "truncated": "5-prime", "pseudogene": { "start": 83732, "stop": 84475, "observations": { "mutation": [ 83900, 83957, 84017 ], "stop": [ 83900, 83957, 84017 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PR70", "query_cov": 1.0, "subject_cov": 0.4388059701492537, "identity": 1.0, "score": 289.0, "evalue": 4.14e-96, "gene_start": 1, "gene_end": 147, "reference_start": 189, "reference_end": 335, "reference_sequence": "MQLEIINPESTLVNDVVAHRVAFSIGSNFNIYWYGIIFVCGFLIAILTYSLRLKFHYKVPYDPGFYYIFLAIPMTIIGARLWSLAIGDAKDFFDFRSGGLAIQGGVIAGVLSAAVYFPLILRMPKYHIRDIDADGNVVIRQPSMWIYADAIIPTILIGQALGRWGNFINGEIFGAESTVNDLQWLKKAMPAVFEGMKHYFIEGNKTLFTIYQPLFLYESFFNVIVFVFIYFGLSYIKQLKIGFISMSYFFFYGVTRFSTESARAPQFSFEGTYIINSLLLIFGVLGALYVQFIAPLLRKKFLLDAIIEMFYKKKDQIHKFGELRKPEEFLFYCHK" }, "paralog": false, "identity": 0.9878542510121457, "score": 483.0, "evalue": 7.12e-175, "description": "Internal stop codon at 83900, 83957, 84017. Nonsense mutation around 83900, 83957, 84017.", "psc": { "uniref90_id": "UniRef90_Q9PR70", "gene": "lgt", "product": "prolipoprotein diacylglyceryl transferase", "ec_ids": [ "2.5.1.145" ], "uniref50_id": "UniRef50_Q9PR70", "cog_id": "COG0682", "cog_category": "M", "go_ids": [ "GO:0005886", "GO:0008961", "GO:0009249", "GO:0016020", "GO:0042158" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PR70", "EC:2.5.1.145", "UniRef:UniRef50_Q9PR70", "COG:COG0682", "COG:M", "GO:0005886", "GO:0008961", "GO:0009249", "GO:0016020", "GO:0042158" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PR70", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR70" ], "product": "Phosphatidylglycerol--prolipoprotein diacylglyceryl transferase" } }, "id": "NKIECMJPJD_159", "locus": "GCA000828735_00123" }, { "type": "cds", "sequence": "contig_1", "start": 84494, "stop": 85420, "strand": "+", "frame": 2, "gene": "trxB", "product": "Thioredoxin reductase", "db_xrefs": [ "COG:COG0492", "COG:O", "EC:1.8.1.9", "GO:0004791", "GO:0005737", "SO:0001217", "UniRef:UniRef50_Q9PR71", "UniRef:UniRef90_Q9PR71" ], "nt": "ATGAATCAAGAAGTTTACGATTTAGTAATCATTGGCGCAGGTCCAGCCGGTTTAGCTGCTGCTGTTTATGCTAAACGTTCTGGATTAAATGTGATCATTGTTGAAAAACAATTCCCAGGAGGAAAAATAGCATTAACATCAAATGTAGAAAATTATTTAGGTATCAATAGTATTCCAGGACCTGAATTGGCTTATAAAATGTACGAACAAGTCTTAAATTTAAATGTTTCTATTATTTATGAAGCTGCTGATGAAATATCTTTAAAAGAAAAATATAAAAAAATTAAATTAACTACACAAACATTAATTACTAAAACTGTAATTATTGCAACAGGAACAGAAAATAGACGGTTAAACATTTTAGGTGAATTAGAATTTGAAAACAAGGGAATTAGTTATTGCGCTATTTGTGATGGACCACTTTATAAAAATAAAGCTGTCTCTGTGATTGGATCTGGTAACTCTGCTGTTGAAGAGGCCATATATTTAGCTACAATTGCTAAAGAAGTTCATTTAATTGCCAATAAACCACAATTTAAAGCAGAACAACAATTAGTACAAATTGCAAATAACACACCTAATATAAAAATTTATTACAATAAACAAACATTCGAATTTTTTGGTCATCAGTTTTTAGAAGGTTTAAAATTTAGAGATTTAATCACAAACGAAGTAACTACACTAAATATTGAAGCTAATTTCACTTTTATTGGCCTATTGCCTTCAAGAATTAATACAAATAATTTATGTATTTTTAATGAGGTAAACGGATTTATTACAACTGATAAAAATATGCAAACTAGTGTTTGTGGTATTTTTGCAGCCGGTGATATTGTTGATAAAAATGTTCGACAAATTGCGACTGCTACAAATGATGGAGTCATTGCAGCACTTTATGCCAAAGAGTACATTACAAGAAATAATTGA", "aa": "MNQEVYDLVIIGAGPAGLAAAVYAKRSGLNVIIVEKQFPGGKIALTSNVENYLGINSIPGPELAYKMYEQVLNLNVSIIYEAADEISLKEKYKKIKLTTQTLITKTVIIATGTENRRLNILGELEFENKGISYCAICDGPLYKNKAVSVIGSGNSAVEEAIYLATIAKEVHLIANKPQFKAEQQLVQIANNTPNIKIYYNKQTFEFFGHQFLEGLKFRDLITNEVTTLNIEANFTFIGLLPSRINTNNLCIFNEVNGFITTDKNMQTSVCGIFAAGDIVDKNVRQIATATNDGVIAALYAKEYITRNN", "aa_hexdigest": "9f67ecbfb6ac62f0b83559f0e2646b49", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_Q9PR71", "query_cov": 1.0, "subject_cov": 0.9967637540453075, "identity": 1.0, "score": 602.0, "evalue": 1.39e-217, "valid": true, "gene": "trxB", "product": "Thioredoxin reductase", "ec_ids": [ "1.8.1.9" ], "uniref50_id": "UniRef50_Q9PR71", "cog_id": "COG0492", "cog_category": "O", "go_ids": [ "GO:0005737", "GO:0004791" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PR71", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR71" ], "product": "Thioredoxin reductase" }, "genes": [ "trxB" ], "id": "NKIECMJPJD_160", "locus": "GCA000828735_00124" }, { "type": "cds", "sequence": "contig_1", "start": 85389, "stop": 85568, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCAAAGAGTACATTACAAGAAATAATTGATAATGATTCTAACAAATTCACGTTTAGTGAATTTGTTAAAGAAGATATTTATACTAACACTGATTATTCATCCACAGACTATAAAGTTATTTTATATTCGTTTTTTCGTAATAATTTAACCATTAAAATTGGTCAAAAACTAACTTGA", "aa": "MPKSTLQEIIDNDSNKFTFSEFVKEDIYTNTDYSSTDYKVILYSFFRNNLTIKIGQKLT", "aa_hexdigest": "a0fa39056c81b580a0ac0da3fba9fc49", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6966.738600000001, "isoelectric_point": 5.086430549621581 }, "id": "NKIECMJPJD_161", "locus": "GCA000828735_00125" }, { "type": "cds", "sequence": "contig_1", "start": 85719, "stop": 86381, "strand": "+", "frame": 3, "gene": "whiA", "product": "DNA-binding protein WhiA", "db_xrefs": [ "COG:COG1481", "COG:K", "GO:0003677", "GO:0043937", "SO:0001217", "UniRef:UniRef50_Q9PR72", "UniRef:UniRef90_Q9PR72" ], "nt": "TTGGATAAATTAGATGAAATTTTAAAACTATTTGATGATGCGAATAATGAAAATTTTCATAAAGACCGCTATTGTTCAAATTTTTTAATTGGTGCAATGTTGTCTGGTGGTTCAATTGCACACCCACTTGAAAACTATCATTTAGAAATTCGCTGTGATTCAAATAATTACATCCCATTATTAACAAAGGCTTTAGCCCGTTATGGTTTAGAATATAAAATTATTTATCGTAATAAAAAAACAATTGTTTATTTTAAAAAATCAGAGATAATCAGTGATTTTTTAAAAGCGATCCGTACGCAAAATTCGTTATTTGAATTTGAGAATATTCGTATCCAACGTGATTTTAATAATCAACAACAACGTTTAAATAATTTAGATATTTCTAACCTTTCTAAAAGTTCTAAAGCAGGGGTTTTGACTAAAGAAATGATTTTAGAAATAAAAAAAAACCACGATGAATTTTGCAAACAAAGTACTAAATTTTTAAAGTATTGTGAACTACGAGTACAAAATCCAGACTGTTCATTAAATGAACTTTCTTGTTTATTAAAACAGATGTTTAATATTGAAATTTCAAAAAGTGGTTTAAATCATTTTAATTCTCGTATTAAAGAAATGTATGAAGAATTAAAATTAAAAAAACTAGTTTTTAAAAACTAG", "aa": "MDKLDEILKLFDDANNENFHKDRYCSNFLIGAMLSGGSIAHPLENYHLEIRCDSNNYIPLLTKALARYGLEYKIIYRNKKTIVYFKKSEIISDFLKAIRTQNSLFEFENIRIQRDFNNQQQRLNNLDISNLSKSSKAGVLTKEMILEIKKNHDEFCKQSTKFLKYCELRVQNPDCSLNELSCLLKQMFNIEISKSGLNHFNSRIKEMYEELKLKKLVFKN", "aa_hexdigest": "535f555a383b4044bdcfe95e331949dc", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "whiA" ], "truncated": "5-prime", "pseudogene": { "start": 85419, "stop": 86381, "observations": { "mutation": [ 85566, 85710 ], "stop": [ 85566, 85710 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PR72", "query_cov": 1.0, "subject_cov": 0.6666666666666666, "identity": 0.995, "score": 435.0, "evalue": 1.59e-152, "gene_start": 1, "gene_end": 220, "reference_start": 111, "reference_end": 330, "reference_sequence": "MPKSTLQEIIDNDSNKFTFSEFVKEDIYTNTDYSSTDYKVILYSFFRNNLTIKIGQKLTWIIKSQNLQIIEFILNGLDYFNNLNIEYEIMFEPDHLNKTRTNYSLLLWGDLDKLDEILKLFDDANNENFHKDRYCSNFLIGAMLSGGSIAHPLENYHLEIRCDSNNYIPLLTKALARYGLEYKIIYRNKKTIVYFKKSEIISDFLKAIRTQNSLFEFENIRIQRDFNNQQQRLNNLDISNLSKSSKAGVLTKEMILEIKKNHDEFCKQSTKFLKYCELRVQNPDCSLNELSCLLKQMFNIEISKSGLNHFNSRIKEMYEELKLKKLVFKN" }, "paralog": false, "identity": 0.99375, "score": 636.0, "evalue": 2.61e-234, "description": "Internal stop codon at 85566, 85710. Nonsense mutation around 85566, 85710.", "psc": { "uniref90_id": "UniRef90_Q9PR72", "gene": "whiA", "product": "DNA-binding protein WhiA", "uniref50_id": "UniRef50_Q9PR72", "cog_id": "COG1481", "cog_category": "K", "go_ids": [ "GO:0003677", "GO:0043937" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PR72", "UniRef:UniRef50_Q9PR72", "COG:COG1481", "COG:K", "GO:0003677", "GO:0043937" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PR72", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR72" ], "product": "Probable cell division protein WhiA" } }, "id": "NKIECMJPJD_162", "locus": "GCA000828735_00126" }, { "type": "cds", "sequence": "contig_1", "start": 86399, "stop": 86548, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGTAAATATATTTCTAAAATTAATAAAAATCGCGCATTTAAAAACCGCCAATTAATTAACACTCCTAATATGTATATTGAAACTTTTAAAGATGTAAGAACAAATCAATTTGCCTTTTTAATTGAAAAAAAGATTAATCAATCCTAA", "aa": "MGKYISKINKNRAFKNRQLINTPNMYIETFKDVRTNQFAFLIEKKINQS", "aa_hexdigest": "5800907ead17b0865abd405b8f6514e6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5895.813700000001, "isoelectric_point": 10.288783454895018 }, "id": "NKIECMJPJD_163", "locus": "GCA000828735_00127" }, { "type": "cds", "sequence": "contig_1", "start": 86630, "stop": 86962, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00571.33" ], "nt": "TTGGTTGTAGTCAATAAAAACTATGAGGTTGTTGGTATTATTTATTTAAAAAAAATTATTAAATATCTTCTTAACGAGCCTAATAAAATCATTAGAACTATCATGCAAACACCTTTATTAATAAACCAAAAAGATACATTATATGATGGTTTAAGAACAATGCAATTTCAAAGAATTCATTTAGGAATTGTTGTTGATAATATTACTGAAAAAAATCCTATAGGAATCATTACATTAGAAAACATTACTGAAGAATTAACTGGTAATGTTTATGATGAAACTGATAATGAGAACGAAATAAAAGAAATTCAAATCGTTAATGAAACAACTTGA", "aa": "MVVVNKNYEVVGIIYLKKIIKYLLNEPNKIIRTIMQTPLLINQKDTLYDGLRTMQFQRIHLGIVVDNITEKNPIGIITLENITEELTGNVYDETDNENEIKEIQIVNETT", "aa_hexdigest": "31413002237690d0a765036870459a1a", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00571.33", "name": "CBS", "length": 59, "aa_cov": 0.509090909090909, "hmm_cov": 0.9322033898305084, "evalue": 1.7552424603278912e-10, "score": 36.655921936035156, "start": 31, "stop": 86 } ], "seq_stats": { "molecular_weight": 12760.62289999999, "isoelectric_point": 4.819230461120605 }, "id": "NKIECMJPJD_164", "locus": "GCA000828735_00128" }, { "type": "cds", "sequence": "contig_1", "start": 87221, "stop": 87517, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01595.25" ], "nt": "ATGATTAATAATTATACAATTACCTTAGCAACCATCTTAATTGGTAACACATTAGTAAATACAGCATCATCAACAATCGCAGGTTTATTTTTTGCAGGTATAATGAAATCTTTTAGTACGACAAGTAATGCTGAAGCTATTGGTACAGGAATTGCAACAGGAATTATAACTTTAATTGTTTTAATATTTGGCGAATTTATTCCTAAAAATTTTGCTAAGAAAAATCCGATTAAAGTTCTTAAATTAGTAGCTTATCCCTTAAATTGCTTTTATATACTTTTTTGGCCATTAACTTGA", "aa": "MINNYTITLATILIGNTLVNTASSTIAGLFFAGIMKSFSTTSNAEAIGTGIATGIITLIVLIFGEFIPKNFAKKNPIKVLKLVAYPLNCFYILFWPLT", "aa_hexdigest": "269420fe648ad2d44afe47ee4122e33e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01595.25", "name": "CNNM", "length": 97, "aa_cov": 0.9897959183673469, "hmm_cov": 0.9278350515463918, "evalue": 4.256010582414005e-27, "score": 90.50971221923828, "start": 1, "stop": 97 } ], "seq_stats": { "molecular_weight": 10591.528599999983, "isoelectric_point": 9.519095420837402 }, "id": "NKIECMJPJD_165", "locus": "GCA000828735_00129" }, { "type": "cds", "sequence": "contig_1", "start": 87960, "stop": 88175, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCATAAGAAAACAAAAAAATATTTAATTAGTATGTTGGCAACAATTTCTATTGTAATTGGTACAACAACAATTATTAGTTCATGTACAAAAAAAACATCTGCAAACGTTCAAGAACAAATCAAATACGATATAATGCCAACTTATACTTCTCAGGCTGATAATTTATTAGCGTTAGGAATTACACCTGATTATTATCCTCAACAAATGTATTGA", "aa": "MHKKTKKYLISMLATISIVIGTTTIISSCTKKTSANVQEQIKYDIMPTYTSQADNLLALGITPDYYPQQMY", "aa_hexdigest": "66288898504da054bab17e70581ec89f", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8008.285200000004, "isoelectric_point": 9.099406242370605 }, "id": "NKIECMJPJD_166", "locus": "GCA000828735_00130" }, { "type": "cds", "sequence": "contig_1", "start": 88410, "stop": 88793, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTGATAATAGTCATTTTGAACGTGCAAAAAAAACATTAGTTGCACATGGTGGCCCGATATCAAATCAAAAAACTGCTATTCCTGTTGATTTTAAAATGAGTCGTGATTTTTATTTAACATTAAATAAAAATGACATTTTAAATTTTAAAAACGATCCTAATTCATTATTACGTAAAAGTCTATTATTAGGTTTAGAGTATAAGGATAAAGAAAAAGGTGAATTAAACGAACTTTATAATTATTCCTATTTTGCTGATAAATTAAACAAAAACTATTTTAATGCTGAAACATTTGATGGTATTAATTTTAATAATTCAGCTTTTAAAAAAAGAATTTTAGAAGGTAATGATATACCTATTTTTTATACTGAAACAAAATGA", "aa": "MIDNSHFERAKKTLVAHGGPISNQKTAIPVDFKMSRDFYLTLNKNDILNFKNDPNSLLRKSLLLGLEYKDKEKGELNELYNYSYFADKLNKNYFNAETFDGINFNNSAFKKRILEGNDIPIFYTETK", "aa_hexdigest": "c89df5d4994d11d6186424f341a14409", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14799.562699999982, "isoelectric_point": 8.6880334854126 }, "id": "NKIECMJPJD_167", "locus": "GCA000828735_00131" }, { "type": "cds", "sequence": "contig_1", "start": 88845, "stop": 88979, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTAACAAAAAAAGTTAGGGAATCAGGATTAAATAAATTAAATTATGATCCTTTTTTATCAACTACAAAACAAGATAGTTATGTTACATCAATTCTACAACACCATCCTATTTATGAACAAAATATGCGATGA", "aa": "MLTKKVRESGLNKLNYDPFLSTTKQDSYVTSILQHHPIYEQNMR", "aa_hexdigest": "88988f544e609fa9f22c254ee38d8b9b", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5225.909799999999, "isoelectric_point": 9.223765754699706 }, "id": "NKIECMJPJD_168", "locus": "GCA000828735_00132" }, { "type": "cds", "sequence": "contig_1", "start": 89010, "stop": 89549, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCGCGATTCATTGCTTTATTTATATGATATTGCTTATGCAACAACAAAATATGCATACTCAGAAGAAGCAAAAATTGATTTTAAAGATAATTTAGCAAAATTAGAACCATTAAAAAAAGCGTTATTTAATGCTAATCAAATTGCTAATAATTTAATTAATCGCTTAAACAAAATGCGTTTGTATTTTCAAGCAATTGGCGTTGTTGATAAAAATTATAATCCTAATATAAAACAATTTGATAATACTAATTCCAAAACTTTAGGCTTATTAACTACATCACAAAATAGTGGTTCTTCAACTTTACAAACTCAATCTAAATACGGTTTTTTATATTATGATTTAGGGTTTAAAGCACCTAAACCAATCTTAAAAAAGGACGAATGGACTGAATTATTAGAAGATGTTAACGGCAAACAATTATTATGTCATAGACACGGAAATGGACAAATTCATTGTTTTGGTGGTGATAATCAAGATGGGACACAAATTGATAAAAACATTGTTGGTTCTCTATTTAATATGGATGATAATGGTTGA", "aa": "MRDSLLYLYDIAYATTKYAYSEEAKIDFKDNLAKLEPLKKALFNANQIANNLINRLNKMRLYFQAIGVVDKNYNPNIKQFDNTNSKTLGLLTTSQNSGSSTLQTQSKYGFLYYDLGFKAPKPILKKDEWTELLEDVNGKQLLCHRHGNGQIHCFGGDNQDGTQIDKNIVGSLFNMDDNG", "aa_hexdigest": "c0f47a5e6d51d3d4298013c8e15328a7", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 20316.668799999992, "isoelectric_point": 7.698693656921387 }, "id": "NKIECMJPJD_169", "locus": "GCA000828735_00133" }, { "type": "cds", "sequence": "contig_1", "start": 89796, "stop": 89903, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTTTAGATTCTGTGATTAAAATTATTAATAAAACAATTGATCCGCAATTAATCCAACAAAATAAACAATTGTTAAAAGAAGCTAATGATTGGGGTTCATATTGA", "aa": "MILDSVIKIINKTIDPQLIQQNKQLLKEANDWGSY", "aa_hexdigest": "72fd0c41a4cb5f6a038fd320d310d612", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4071.694300000001, "isoelectric_point": 5.91923542022705 }, "id": "NKIECMJPJD_170", "locus": "GCA000828735_00134" }, { "type": "cds", "sequence": "contig_1", "start": 89946, "stop": 90659, "strand": "+", "frame": 3, "gene": "fepD", "product": "Hemin transport system permease", "db_xrefs": [ "COG:COG0609", "COG:P", "SO:0001217", "UniRef:UniRef50_Q9PR75", "UniRef:UniRef90_Q9PR75" ], "nt": "ATGAAAATTAATCAAAAAACGAATTTAAAGCAAAAGCATTTTTATTTAATTAAAAATCTTAATTATAAACAATCTGTAATCATTAAAATGGCAGGACTAATTCTCATTGCCATTTGTTTTGTTTTTTTAACATTAGCAATTTATTTTGATACCACTAATGGTTTTGAAGCATCGATCAATCAATTTAATTTTAATATTCGTGGTCCTAAAACTTTAATGGCCTTTTTTTCAGGAGGAGCAATTGCTGTAGCTGGATTATTATTACAAAAAGTTACACGTAATCATTTAGCAGATACGTCAGTTTTAGGTATTGGTTCATTAAACATCATTTTAATGACAGTATATTTAATTCTTATTAAAGATAAAACAAGCAGTTATTTAAATCCTGAAATGCGAATTTTACCCTTGGTGATGTTTTTATCATCAATTTTTGGAACAAGTATTGTTTATACATTATCAAAATTAGGAAGAGCTAATAATGAAAAATTTATTATTATCGGAATTGCTTTGAATTTTTTATTTGAAGCGATTTCCGTGGTTTTAATTAATCCTGCTAATAATTCATTGTTCTCCAATCAAGATAAAAATTTAATGATTGCTTTTGAAAATATCAAAAATTATACTTTAGGAATTATTCCAAATTCGCAATATATTGATTTATCATTAGTAATCTCTTGCTCAGTATTAATTGCATTAACATTGATTCCAATTTGA", "aa": "MKINQKTNLKQKHFYLIKNLNYKQSVIIKMAGLILIAICFVFLTLAIYFDTTNGFEASINQFNFNIRGPKTLMAFFSGGAIAVAGLLLQKVTRNHLADTSVLGIGSLNIILMTVYLILIKDKTSSYLNPEMRILPLVMFLSSIFGTSIVYTLSKLGRANNEKFIIIGIALNFLFEAISVVLINPANNSLFSNQDKNLMIAFENIKNYTLGIIPNSQYIDLSLVISCSVLIALTLIPI", "aa_hexdigest": "5d7eae396c0675dcd627e8586de80ca2", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "fepD" ], "truncated": "3-prime", "pseudogene": { "start": 89946, "stop": 90962, "observations": { "mutation": [ 90657 ], "stop": [ 90657 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PR75", "query_cov": 1.0, "subject_cov": 0.6657303370786517, "identity": 0.92, "score": 409.0, "evalue": 1.75e-141, "gene_start": 1, "gene_end": 237, "reference_start": 1, "reference_end": 237, "reference_sequence": "MKINYKTKLKQHHFYLIKNLNYKQFAIIKMAGLIAIALCFIFLTLAIYYDTTNGFDVSINQFNFNIRGPKTLMAFFSGGAIAVAGLLLQKVTRNHLADTSVLGIGSLNIILMTFYFILIKDKTSSYLNPEMRILPLIMFLSSILGTSIVYTLSKLGKANNEKFIIIGIALNFLFEAVSVVLINPANNSLFSNQDKNLMIAFENIKNYTLGIIPNSQYIDLSLVISCSILITLTLIPIWFLRKKIDLYETSAALASTTGINVERLKISIYFLVALLAGLEIVLVGYVALLGVIGASVARQLFGNKTGITMFAAFLIGAILVCFALLISVNFNTQVPVGFLSTTIITPYFMYLIVRGK" }, "paralog": false, "identity": 0.9260355029585798, "score": 604.0, "evalue": 4.06e-221, "description": "Internal stop codon at 90657. Nonsense mutation around 90657.", "psc": { "uniref90_id": "UniRef90_Q9PR75", "gene": "FepD", "product": "Hemin transport system permease", "uniref50_id": "UniRef50_Q9PR75", "cog_id": "COG0609", "cog_category": "P", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PR75", "UniRef:UniRef50_Q9PR75", "COG:COG0609", "COG:P" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PR75", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR75" ], "product": "Hemin transport system permease" } }, "id": "NKIECMJPJD_171", "locus": "GCA000828735_00135" }, { "type": "cds", "sequence": "contig_1", "start": 90759, "stop": 91016, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGCTTTATTAGCAGGATTAGAAATTGTTTTAGTTGGTTATATAGCTTTATTAGGAGTTATTGGCGCTAGCGTTGCACGTCAATTATTTGGTAATAGAACAGGTATTACAATGTTTGCAGCATTTTTAATCGGCGCAACTTTAGTGTGTTTTGCGTTATTAATTTCGGTTAATTTTAATACACAAGTACCAGTTGGTTTTTTATCAACAACAATTATTACACCTTATTTTATGTATCTAATTGTGAGAGGAAAATAA", "aa": "MALLAGLEIVLVGYIALLGVIGASVARQLFGNRTGITMFAAFLIGATLVCFALLISVNFNTQVPVGFLSTTIITPYFMYLIVRGK", "aa_hexdigest": "92d297ec9efb3e15d61c63c2d8442133", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9056.890599999993, "isoelectric_point": 9.622825050354006 }, "id": "NKIECMJPJD_172", "locus": "GCA000828735_00136" }, { "type": "cds", "sequence": "contig_1", "start": 91021, "stop": 91896, "strand": "+", "frame": 1, "gene": "fepC", "product": "Iron(III) dicitrate transport ATP-binding protein", "db_xrefs": [ "COG:COG1120", "COG:PH", "RefSeq:WP_006689300.1", "SO:0001217", "UniParc:UPI00016BFF62", "UniRef:UniRef100_A0AAC9T2Y7", "UniRef:UniRef50_Q9PR76", "UniRef:UniRef90_Q9PR76" ], "nt": "ATGTTTAAAATTTTTGCTAAATACTTTTGCAATAATCAATTAGATCGGAATCAAATTATTAATGCAAGTAAAAACGACGCTTTAATTATTAATAATTTATCTTATAGATATCACAAAAAACTTCCTGAAGTTTTAAAAAATTTGTCATTTAGTATTAAAAAAAATTCATTTACAACAATTTTAGGACCAAATGGATGTGGTAAGTCTACAACAGCGAAAGCGATTGTTAAATTATTAAAACTTAAAAAAAATATTATTAAAGTGTTTGATAGAAACATTAATGAATTAAGTTTTAAACAATTAGCACAATTAATTTCTTATATTCCTCAAAGTATTGAAATTCCCCAAGGCACTCGTGTTATTGATTTTATTACGTTTGGTCGTAATCCATATTTAGGATTTAGTGGCATTTTAGGAAAAGAAGATAAATTAATTATTGATTTAGTAATTAATGAAATGCAACTGCACGATTTAAAAGAAAAATTTATGCAAGAATTATCTGGTGGTCAGCGCCAAAAAGTTATTTTAGCATTGTGTTTAGTTCAAGATACTCCAATTATTTTATTAGATGAACCAACTACATATTTAGATATTAAAAATCAATATGAATTACTTGAATCACTAAAAAAATTACAAATTTTACGTCAAAAAACAATTATTGCAATTTTACATGATATTAATCAAGCTATTCAATATAGTGATGAAGTTTTTGTCTTAAAAAATGGGCAAATCTATGCAAATGGTAATCCAAATGAAGTTATTACTAAAGAGTTATTAAAAAACGTTTATAACATTGATGCTCAAATTGATATTGTTGATAATCAAAAAATTGTTCATAAGATTAAAGTTAAAGATTATTTATCAAATAGTGAATAA", "aa": "MFKIFAKYFCNNQLDRNQIINASKNDALIINNLSYRYHKKLPEVLKNLSFSIKKNSFTTILGPNGCGKSTTAKAIVKLLKLKKNIIKVFDRNINELSFKQLAQLISYIPQSIEIPQGTRVIDFITFGRNPYLGFSGILGKEDKLIIDLVINEMQLHDLKEKFMQELSGGQRQKVILALCLVQDTPIILLDEPTTYLDIKNQYELLESLKKLQILRQKTIIAILHDINQAIQYSDEVFVLKNGQIYANGNPNEVITKELLKNVYNIDAQIDIVDNQKIVHKIKVKDYLSNSE", "aa_hexdigest": "087e318420cbf963de64d5b099295044", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI00016BFF62", "ncbi_nrp_id": "WP_006689300.1", "uniref100_id": "UniRef100_A0AAC9T2Y7", "db_xrefs": [ "SO:0001217", "UniParc:UPI00016BFF62", "RefSeq:WP_006689300.1", "UniRef:UniRef100_A0AAC9T2Y7" ] }, "ips": { "uniref100_id": "UniRef100_A0AAC9T2Y7", "uniref90_id": "UniRef90_Q9PR76" }, "psc": { "uniref90_id": "UniRef90_Q9PR76", "gene": "FepC", "product": "Iron(III) dicitrate transport ATP-binding protein", "uniref50_id": "UniRef50_Q9PR76", "cog_id": "COG1120", "cog_category": "PH" }, "pscc": { "uniref50_id": "UniRef50_Q9PR76", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR76" ], "product": "Iron(III) dicitrate transport ATP-binding protein" }, "genes": [ "fepC" ], "id": "NKIECMJPJD_173", "locus": "GCA000828735_00137" }, { "type": "cds", "sequence": "contig_1", "start": 91987, "stop": 92460, "strand": "+", "frame": 1, "gene": null, "product": "MPN555 family protein chaperone", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A9E2NXK8", "UniRef:UniRef90_A0AAC9T382" ], "nt": "ATGAATTTTAAAAGTACCATCGTTAATAAAAAACCGATTGATTGGAAACACACAATTGTTTTAGAAGAATTAAATGTTGATCCTAAAGCATTAGAAATGCATAAAGAAAGAATTAACACAGTTTTTGCAAAACAAACTGAAGAACAACGTTCTCAACAATTACATAACATTATTGTTCGTGAAAATTTGTTTAATAAAGCAATGACATATCTTGCTGATTTTTATGAAATTGATGTTAATGAAGAAGATATTAAAGATTTAGCACCACGTATTAAACAAGCATTTGGTGTTGAAGATGAAAAGTTAGCTTATGAAATTGCACAAAAAATCATCGCTAAAGCATTAATTTTTCAAGATTTACAAAAAGAATTTACAATTGAAATTAAAGATGAAGAATTAACAAAAATCCTTGAATCATACTATGAAGAGACAAACTTATCAATTCGTGACTTTAAAGAAAACAAAGCACAATGA", "aa": "MNFKSTIVNKKPIDWKHTIVLEELNVDPKALEMHKERINTVFAKQTEEQRSQQLHNIIVRENLFNKAMTYLADFYEIDVNEEDIKDLAPRIKQAFGVEDEKLAYEIAQKIIAKALIFQDLQKEFTIEIKDEELTKILESYYEETNLSIRDFKENKAQ", "aa_hexdigest": "c07c5f175cf9262b65d5ee609ce2b8c7", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 91987, "stop": 92643, "observations": { "mutation": [ 92458 ], "stop": [ 92458 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAC9T382", "query_cov": 1.0, "subject_cov": 0.7201834862385321, "identity": 0.955, "score": 291.0, "evalue": 1.1e-98, "gene_start": 1, "gene_end": 157, "reference_start": 1, "reference_end": 157, "reference_sequence": "MNFKSTIINKKPIDWKHTIVLEELSVDPKALEMHKERINTVFAKQTEEQRAQQLHNIIVRENLFNKAMTYLADFYEIDVNEEDVKDLAPRIKQAFGVEDEKLAYEISQKIIAKALIFQDLQKEFNIEIKDDELTKILESYYEETNLSIRDFKENKAQWEAAKSTLLEEKTTAFIVDKFDRDLSILEANIRKKIAEQMELDKKIKEVQDNSKAKQNADK" }, "paralog": false, "identity": 0.9495412844036697, "score": 409.0, "evalue": 2.5e-147, "description": "Internal stop codon at 92458. Nonsense mutation around 92458.", "psc": { "uniref90_id": "UniRef90_A0AAC9T382", "product": "MPN555 family protein chaperone", "uniref50_id": "UniRef50_A0A9E2NXK8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAC9T382", "UniRef:UniRef50_A0A9E2NXK8" ] }, "pscc": { "uniref50_id": "UniRef50_A0A9E2NXK8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A9E2NXK8" ], "product": "Trigger factor C-terminal domain-containing protein" } }, "id": "NKIECMJPJD_174", "locus": "GCA000828735_00138" }, { "type": "cds", "sequence": "contig_1", "start": 92681, "stop": 93295, "strand": "+", "frame": 2, "gene": null, "product": "HTH gntR-type domain-containing protein", "db_xrefs": [ "RefSeq:WP_006689287.1", "SO:0001217", "UniParc:UPI00016BFF61", "UniRef:UniRef100_A0AAC9T052", "UniRef:UniRef50_A0A2C9DZ67", "UniRef:UniRef90_A0A2C9DZ67" ], "nt": "ATGGATCAATATAAAATAAATGAATTGATTCCTAGTGAGTATAAACTAGCAAGTTATTTTAATTGTGGACGAATCACTATTCATAATGCTTATGAAGTATTAAAAGTTTTAGGGATTGTAACAACAATCAAAGGAAGTGGTTATTATGTTTATTCAAGTATTGATCACTTTACTAATAAGGCTTTTGCTAATTTATACAAAACTATTGATAATATTAAATACAAACAACTTCTAATTGATTTTGAATATCATGGTAATAAGTTTAATTCAATCACTAAATTTAGTTTAATTAAAAATAAAAAAATAATTGCAGTATCATACTATATAACACCAAATAATATGGATTTAGAACAATTCGATACTAATAACATTAATATTAGTAAACAATTAATCCTCTCTGGTTTTGATGATTTTTTTAATGTAAAAACACAAATTAATTATGAAAATATTAATTTATTAAAATCAATTTTAGAAGATGAAAATATTAAATTTGACACACCAATTATTTTAAATAAATTAACTTCATCATCATTAAAAGTAGAATTATTAGTTTTAACACACATTAGCAAAGAATATTTTGATTATGAAGAGACTAATAAATTAATTATTAATTAA", "aa": "MDQYKINELIPSEYKLASYFNCGRITIHNAYEVLKVLGIVTTIKGSGYYVYSSIDHFTNKAFANLYKTIDNIKYKQLLIDFEYHGNKFNSITKFSLIKNKKIIAVSYYITPNNMDLEQFDTNNINISKQLILSGFDDFFNVKTQINYENINLLKSILEDENIKFDTPIILNKLTSSSLKVELLVLTHISKEYFDYEETNKLIIN", "aa_hexdigest": "48ed4285459781502c52ab05b10a0c5d", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00016BFF61", "ncbi_nrp_id": "WP_006689287.1", "uniref100_id": "UniRef100_A0AAC9T052", "db_xrefs": [ "SO:0001217", "UniParc:UPI00016BFF61", "RefSeq:WP_006689287.1", "UniRef:UniRef100_A0AAC9T052" ] }, "ips": { "uniref100_id": "UniRef100_A0AAC9T052", "uniref90_id": "UniRef90_A0A2C9DZ67" }, "psc": { "uniref90_id": "UniRef90_A0A2C9DZ67", "product": "HTH gntR-type domain-containing protein", "uniref50_id": "UniRef50_A0A2C9DZ67" }, "pscc": { "uniref50_id": "UniRef50_A0A2C9DZ67", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2C9DZ67" ], "product": "HTH gntR-type domain-containing protein" }, "genes": [], "id": "NKIECMJPJD_175", "locus": "GCA000828735_00139" }, { "type": "cds", "sequence": "contig_1", "start": 93342, "stop": 93914, "strand": "-", "frame": 1, "gene": "pta", "product": "Phosphotransacetylase", "db_xrefs": [ "COG:C", "COG:COG0280", "SO:0001217", "UniRef:UniRef50_Q9PR79", "UniRef:UniRef90_Q9PR79" ], "nt": "GTGGGTGCTAACTTGTCATCAAAAACTTGTTTTAGTAGTATCTTAAAGATTTTGAAAAATCCTAACGGTTCGATCGCATATAGTACAATGGTTATGATTAATAAACGAGAATTAATTTTTATGAGTGATTGTGCTTTAAATTTAAACTTGAATAAACAACAAATGATTGATGTAGCAAAATATTCATGCACAATTAGTAGAAATTATTTTGATGTTTTAAACAATCATGTGGGATATATTAGTTATTTAAGTCAAAATGATGTTATTTATCAAAATAAAAATTTTGATTTTATTGGCCCAATTCAATTCGATGCTGTAGTTGATTTAAAAGTTCGTAATAAAAAAGTACCTAATATTCATCAATACCATATTAATCATTATGTTTTTGATAATATAAGTATTGCAAATACTGTTTTTAAAATTTATCAAACAAAACTTAATTATTTAACAGTCGGTACATATATTAGTAATATCTTAACAAAAGTTAGTGTTATTAGTCGTTCAGCAAGTAGTGATGAAATTATTGCAACAACTTATTTATTATGCGTGGCTTTTTTAACAGATTTAACTTAA", "aa": "MGANLSSKTCFSSILKILKNPNGSIAYSTMVMINKRELIFMSDCALNLNLNKQQMIDVAKYSCTISRNYFDVLNNHVGYISYLSQNDVIYQNKNFDFIGPIQFDAVVDLKVRNKKVPNIHQYHINHYVFDNISIANTVFKIYQTKLNYLTVGTYISNILTKVSVISRSASSDEIIATTYLLCVAFLTDLT", "aa_hexdigest": "6a95add6d6f53735748717d5c11f3604", "start_type": "GTG", "rbs_motif": null, "genes": [ "pta" ], "truncated": "3-prime", "pseudogene": { "start": 93342, "stop": 94214, "observations": { "mutation": [ 94031 ], "stop": [ 94031 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PR79", "query_cov": 1.0, "subject_cov": 0.6484641638225256, "identity": 0.995, "score": 367.0, "evalue": 8.02e-127, "gene_start": 1, "gene_end": 190, "reference_start": 104, "reference_end": 293, "reference_sequence": "MLSSINKLKNKLKNLAKKPIKVLFIEQSLEIDKAKKMLSQDTLINIVDLSSFKTPEIINECEEIWYELRKLKGESRNDAQIAVNDNLNFAVCLSYLKKIDVLIVGANLSSKTCFSSILKILKNPNGSIAYSTMVMINKRELIFMSDCALNLNLNKQQMIDVAKYSCTISRNYFDVLNNHVGYISYLSQNDVIYQNKNFDFIGPIQFDAVVDLKVRNKKVPNIHQYHINHYVFDNISIANTVFKIYQTKLNYLTVGTYISNILTKVSVISRSASSDEIIATTYLLCVAFLTDLT" }, "paralog": false, "identity": 0.996551724137931, "score": 564.0, "evalue": 4.37e-207, "description": "Internal stop codon at 94031. Nonsense mutation around 94031.", "psc": { "uniref90_id": "UniRef90_Q9PR79", "gene": "Pta", "product": "Phosphotransacetylase", "uniref50_id": "UniRef50_Q9PR79", "cog_id": "COG0280", "cog_category": "C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PR79", "UniRef:UniRef50_Q9PR79", "COG:COG0280", "COG:C" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PR79", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR79" ], "product": "Phosphotransacetylase" } }, "id": "NKIECMJPJD_176", "locus": "GCA000828735_00140" }, { "type": "cds", "sequence": "contig_1", "start": 94029, "stop": 94223, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGAGTTCTATAAATAAGTTAAAAAATAAATTAAAAAATCTAGCAAAAAAACCAATAAAAGTTTTATTTATTGAACAAAGTTTAGAAATTGATAAAGCAAAAAAAATGTTAAGTCAAGATACTTTAATTAATATCGTAGATTTGTCATCTTTTAAAACACCAGAAATTATTAATGAATGTGAAGAGATTTGA", "aa": "MLSSINKLKNKLKNLAKKPIKVLFIEQSLEIDKAKKMLSQDTLINIVDLSSFKTPEIINECEEI", "aa_hexdigest": "ea7be21d7e0f121c2c5a4909c239ebf5", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7330.667299999998, "isoelectric_point": 8.787959480285647 }, "id": "NKIECMJPJD_177", "locus": "GCA000828735_00141" }, { "type": "cds", "sequence": "contig_1", "start": 94659, "stop": 95198, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGACAGATTCTCAATTAAATGTATATACACGCAAGTATAATGAAATTTTTCGTTATCAACCACACACATTAAATAATGAACAAAGTAAGTTATTTTCAACATTAATACGTGCTGATGAAGGTTTTTCAACAATTTTTTCAACATATACAAATAATGATATGAAATTTAGTGATGCTATTGATGCAAAAGGTAAAAAACACCCAATTAAGAATGAAGCAGAAGCTTTTGTTCATTTAAAATCAAAAGATCGTGTTTTGCGTAAAAGCGCTTATTTATCAATGTATAGAGCATATTATGATTCACGTGAATCAATTACAAAAATGTTGTACTACAATTATTTATCATTAAATCAACAAGCAAAAGCAAAAAACTTTGATGATTATATTGCTAAAGCAGCATTTGATGATGTTGTTGACAAATCATTAATTACTTTAATTTATGATCAAGTAAAACTTTATAAAGATACAAATGAAGATTATAAAAAAGTACGAAATACATATTTAAAAAAATTAATTAAAGTTTCAAAGATCGAACCTTGA", "aa": "MTDSQLNVYTRKYNEIFRYQPHTLNNEQSKLFSTLIRADEGFSTIFSTYTNNDMKFSDAIDAKGKKHPIKNEAEAFVHLKSKDRVLRKSAYLSMYRAYYDSRESITKMLYYNYLSLNQQAKAKNFDDYIAKAAFDDVVDKSLITLIYDQVKLYKDTNEDYKKVRNTYLKKLIKVSKIEP", "aa_hexdigest": "30e3c10bc508fa441a800f2599969a01", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 21161.81040000001, "isoelectric_point": 9.33909969329834 }, "id": "NKIECMJPJD_178", "locus": "GCA000828735_00142" }, { "type": "cds", "sequence": "contig_1", "start": 95418, "stop": 95696, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACTATACTAACTCATTACGTGATGTACAAACTATTGTTCATGAATTAGGCCACTCAATGCATTCGTTATATTCTAATCGCACACAAAAAATTTATAGTGATTACAAGATTTTCTATGCGGAAATTGCATCAATTTCAAATGAAGTTTATTTAAACTACTATTTATTAGAAAAATACAAGAACGATCTTGAAATGAAATTAATGATTTTAGATGAAATGATTAGTGGGTTCTTCGCAACAACAACAAGACAAGTAATTTTTTCAAATTTTGAATGA", "aa": "MNYTNSLRDVQTIVHELGHSMHSLYSNRTQKIYSDYKIFYAEIASISNEVYLNYYLLEKYKNDLEMKLMILDEMISGFFATTTRQVIFSNFE", "aa_hexdigest": "651f52f2e3b94c70759e559b1aa14ad4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10984.371599999991, "isoelectric_point": 5.526534843444824 }, "id": "NKIECMJPJD_179", "locus": "GCA000828735_00143" }, { "type": "cds", "sequence": "contig_1", "start": 95748, "stop": 96050, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGAATATCAAAAACTAGAACTTGAATATACTAACAAACCAATTGTAGAGGATCTAAATTCAATTTATTCATTATCAAGCATTACTCCACTTCGTATTCCTCATTTTTATGTAGGTAATTTTTATGTATATAAATATGCAGTAGGTCAAGTTGCAGCAATTATTTCAGGTCATCGTGTTTTCACTAAAGTAACAGGTGCAAAACAAAAAGTGTTTGATTTCTTATCAAGTGGCGGTTCAAAAGATCCATTAGATACAATTAAATTACTAGGTGTTGATTTAACAAAACCACAAGCATGA", "aa": "MKEYQKLELEYTNKPIVEDLNSIYSLSSITPLRIPHFYVGNFYVYKYAVGQVAAIISGHRVFTKVTGAKQKVFDFLSSGGSKDPLDTIKLLGVDLTKPQA", "aa_hexdigest": "75145978bccff77ab21c311f7f44c494", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11201.807899999989, "isoelectric_point": 9.126482963562015 }, "id": "NKIECMJPJD_180", "locus": "GCA000828735_00144" }, { "type": "cds", "sequence": "contig_1", "start": 96383, "stop": 96733, "strand": "+", "frame": 2, "gene": "rCL", "product": "putative protein UU063", "db_xrefs": [ "COG:COG3613", "COG:F", "SO:0001217", "UniRef:UniRef50_Q9PR82", "UniRef:UniRef90_Q9PR82" ], "nt": "ATGAGTAAAAAAATCAAAATTTATTTAGCTGGTCCTTTATTCACCTTAGCTGAAATTAATGATCGAAAACAACAAGCTAGTTTAATTAGAAAAACATTTAAAGATGAATTACCAAATTATGAACTTGATCTTTTTAATCCGATCGAAGTTAACGACGAATTAGGTGCTAATGCACATAAACCAAATATCTTTTTTTATGAATCAGATATTAAATTTATTGATCAAACTGATATCGCAATTATAGATATTGATAACACTGATGATGGCACCATGGCTGAAATGGGTTATTTTGTAGCACTTCAAAAACACGTTAAACCAACGTTAAAAATTTATATTTTAAATACTGATTGA", "aa": "MSKKIKIYLAGPLFTLAEINDRKQQASLIRKTFKDELPNYELDLFNPIEVNDELGANAHKPNIFFYESDIKFIDQTDIAIIDIDNTDDGTMAEMGYFVALQKHVKPTLKIYILNTD", "aa_hexdigest": "58832fd25ca5817917e7926d9c4ea843", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [ "rCL" ], "truncated": "3-prime", "pseudogene": { "start": 96383, "stop": 96859, "observations": { "mutation": [ 96731 ], "stop": [ 96731 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PR82", "query_cov": 1.0, "subject_cov": 0.7341772151898734, "identity": 1.0, "score": 233.0, "evalue": 5.5e-77, "gene_start": 1, "gene_end": 116, "reference_start": 1, "reference_end": 116, "reference_sequence": "MSKKIKIYLAGPLFTLAEINDRKQQASLIRKTFKDELPNYELDLFNPIEVNDELGANAHKPNIFFYESDIKFIDQTDIAIIDIDNTDDGTMAEMGYFVALQKHVKPTLKIYILNTDWRVHKHRNEVLNKFLDGMILSHCQYFTNFTDLLNHLLIELKK" }, "paralog": false, "identity": 0.9936708860759493, "score": 318.0, "evalue": 5.11e-113, "description": "Internal stop codon at 96731. Nonsense mutation around 96731.", "psc": { "uniref90_id": "UniRef90_Q9PR82", "gene": "RCL", "product": "Uncharacterized protein UU063", "uniref50_id": "UniRef50_Q9PR82", "cog_id": "COG3613", "cog_category": "F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PR82", "UniRef:UniRef50_Q9PR82", "COG:COG3613", "COG:F" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PR82", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR82" ], "product": "Uncharacterized protein UU063" } }, "id": "NKIECMJPJD_181", "locus": "GCA000828735_00145" }, { "type": "cds", "sequence": "contig_1", "start": 97142, "stop": 97789, "strand": "-", "frame": 2, "gene": null, "product": "lysine--tRNA ligase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3M1L5W2" ], "nt": "ATGCAACTAACTGAAGATTTAATTCGTCATATTGCTACTAAATTAAACTTATTAAATCCTACATTTAGAGGTTTTAGTATTGATTTAAATAAACCTTTTAAAAAAGCTCATATGGTTGATTTAATTAATGAATATGTTGGTGTTAATTTTTTTGAAGTTAAAAGCGATGAACAAGCAATTGAATTAGCAAAAAAACACCATGTTAAATTATTAGATCATCAAAAAAACTTTGGTCATATCGTTAATGCTTTTTTTGAAACTTTTGTTGAAGAAAAATTAGTTGAACCAACATTTGTATATGGTCATCCGTTACAAGTTTCTCCACTTACTAAAAAGAATCAGAGTGATCCTCGTTTTGTAGATCGTTTTGAATTATTTATTTGTCAAAAAGAATTTGCTAATGCTTATAGTGAAATTAATGATCCAATTGATCAATATGAACGTTTTATCGCACAGTTAGAAGAAGCTAAATTAGGAAATGACGAAGCAAGCGAATTAGATATGGAATTTATTGAGGCCTTAGAATATGGTATGCCTCCAACAGGTGGTTTGGGAATCGGTGTTGATCGTTTAGTAATGCTGTTAACAAGTAATGATAGTATTCGTAATGTTTTATTATTTCCTCACATGAAAGATAAAACAAAATAA", "aa": "MQLTEDLIRHIATKLNLLNPTFRGFSIDLNKPFKKAHMVDLINEYVGVNFFEVKSDEQAIELAKKHHVKLLDHQKNFGHIVNAFFETFVEEKLVEPTFVYGHPLQVSPLTKKNQSDPRFVDRFELFICQKEFANAYSEINDPIDQYERFIAQLEEAKLGNDEASELDMEFIEALEYGMPPTGGLGIGVDRLVMLLTSNDSIRNVLLFPHMKDKTK", "aa_hexdigest": "d9d91c773349180c4b753b797e2196a9", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002E2B0F01", "query_cov": 0.9953488372093023, "subject_cov": 0.8014981273408239, "identity": 0.575, "score": 270.0, "evalue": 1.87e-88, "valid": false, "product": "amino acid--tRNA ligase-related protein", "ec_ids": [ "6.1.1.6" ], "uniref50_id": "UniRef50_A0A3M1L5W2", "kegg_orthology_id": "K04567" }, "pscc": { "uniref50_id": "UniRef50_A0A3M1L5W2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3M1L5W2" ], "product": "lysine--tRNA ligase" }, "genes": [], "id": "NKIECMJPJD_182", "locus": "GCA000828735_00146" }, { "type": "cds", "sequence": "contig_1", "start": 97796, "stop": 98623, "strand": "-", "frame": 2, "gene": null, "product": "Lysyl-tRNA synthetase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A401FP47" ], "nt": "ATGGAAAGAAAATTTAGTGATCAAGAATTAATCCGTCGTACACACTTACAAGAATTAAAAAACCAAAATAAAAATCCTTTTTTAATAACAAAAGTTGATAGAAGTATGTTTTTAAAAGACTTTGCTGAAAAATATAAAAATTTTAGCAAAGAAGAATTACATAATATGGATTTAGAGAAATTGACACTTGCTGGTCGTTTAATCGGAATACGTCAAACCTTTGGGATTATTCAAGATTTTTCAGCAAAGTTACAAATTTATATTAATAAAAAAAATGTTGCTCCAGAGGTTTTTTCAACTTTTAAATCATTAGATATTGGTGATATTATTGAATTAGAAGGTGTAGCAATGAAAACTAATAGTGATGAAATTACTTTAAATGTTACAAACATTCGATTAGTTGCTAAAAGTCTAAAAGTTCTCCCTGAAAAGTATCATGGATTGGTTGATGAAGAAATTAAAGCACGTCAACGTTATTTAGATTTAATTGTTAACGATGAGACAAAAAATACTTTTATAAAACGATCTTTAATTATACGTGAAATGCGTAATTGGTTAGATAATAAAGGTTTTTTTGAAGTTGAAACGCCTGTTTTGCAAGATATTTTAAGTGGTGCTGCTGCTCGTCCTTTTATTACACATCATAACACTTTAAATAAAGAATATTATTTAAGAATTGCTACTGAAATTGCTTTAAAAAAGTGTATTATTGGGGGTTTTGAAAAAGTATATGAAATAGGTAGAATTTTTCGAAATGAGGGAATGGATAGTACTCATAATCCTGAATTTACTTCAGTTGAATTATATATTGCATATGTTGATTTATGA", "aa": "MERKFSDQELIRRTHLQELKNQNKNPFLITKVDRSMFLKDFAEKYKNFSKEELHNMDLEKLTLAGRLIGIRQTFGIIQDFSAKLQIYINKKNVAPEVFSTFKSLDIGDIIELEGVAMKTNSDEITLNVTNIRLVAKSLKVLPEKYHGLVDEEIKARQRYLDLIVNDETKNTFIKRSLIIREMRNWLDNKGFFEVETPVLQDILSGAAARPFITHHNTLNKEYYLRIATEIALKKCIIGGFEKVYEIGRIFRNEGMDSTHNPEFTSVELYIAYVDL", "aa_hexdigest": "2ddb4906a6cf4d4057c804f8402b3f39", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI0029082C91", "query_cov": 0.9781818181818182, "subject_cov": 0.9962962962962963, "identity": 0.513, "score": 245.0, "evalue": 1.35e-77, "valid": false, "product": "amino acid--tRNA ligase-related protein", "ec_ids": [ "6.1.1.6" ], "uniref50_id": "UniRef50_A0A401FP47", "kegg_orthology_id": "K04567" }, "pscc": { "uniref50_id": "UniRef50_A0A401FP47", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A401FP47" ], "product": "Lysyl-tRNA synthetase" }, "genes": [], "id": "NKIECMJPJD_183", "locus": "GCA000828735_00147" }, { "type": "cds", "sequence": "contig_1", "start": 98733, "stop": 98969, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTGCTCCAATCGATTATTTTATTGATCAAAATAATTTAAATAATGTTTTTGATCGTCAAAAACACGCTATTTATAAACTTTATTTGCTTGATTTGATTTTAAATGAATTGTGCCGTTCAATGAATAATGACCATCGTATTAATAATGAATTTAAGTATTTTGATTGCGAAGTTATTTATGTTGAAGATTTATATTTAAAAATTTTATATAAACTTTATTTTCAATTAAATTAA", "aa": "MLAPIDYFIDQNNLNNVFDRQKHAIYKLYLLDLILNELCRSMNNDHRINNEFKYFDCEVIYVEDLYLKILYKLYFQLN", "aa_hexdigest": "56007707b98f68c94b9cf89675339412", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9646.019599999992, "isoelectric_point": 5.127752494812011 }, "id": "NKIECMJPJD_184", "locus": "GCA000828735_00148" }, { "type": "cds", "sequence": "contig_1", "start": 99195, "stop": 99527, "strand": "-", "frame": 1, "gene": null, "product": "putative protein UU061", "db_xrefs": [ "GO:0016020", "SO:0001217", "UniRef:UniRef50_Q9PR84", "UniRef:UniRef90_Q9PR84" ], "nt": "ATGACATCGTTAGACATAACCACGTTAGTAATTAGTATACTTTCGTTAATTGCAACACTATCAATTTCTTTTAATATTTATTTTATTGAATTAAGAAAACAACGCGAACGTAGAATTGAACGTCTACAACAAGAAGCAAAACAATTTATTATTAACAACTTAGATGAAAAAGATTTTATTGTTTTGTGTCAATTTATTTATAAATTATATAAACATGATAAACATACAAGAAAGATTCATTATGAATTTGTTTTATTAAATGAACCTGTTCAAAAAGAGGTCTTTAAACAACTAGAAATTCTAAATATAGTTGATTTTAAAGATAATGAATGA", "aa": "MTSLDITTLVISILSLIATLSISFNIYFIELRKQRERRIERLQQEAKQFIINNLDEKDFIVLCQFIYKLYKHDKHTRKIHYEFVLLNEPVQKEVFKQLEILNIVDFKDNE", "aa_hexdigest": "8d7839f1030de0ad5602d69057029527", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 98892, "stop": 99527, "observations": { "mutation": [ 99197 ], "stop": [ 99197 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PR84", "query_cov": 1.0, "subject_cov": 0.4166666666666667, "identity": 0.991, "score": 203.0, "evalue": 4.73e-64, "gene_start": 1, "gene_end": 110, "reference_start": 1, "reference_end": 110, "reference_sequence": "MTSLDITTLVISILSLIATLSISFNIYFIELRKQRERKIERLQQEAKQFIINNLDEKDFIVLCQFIYKLYKHDKHTRKIHYEFVLLNEPVQKEVFKQLEILNIVDFKDNEWIANKFSILKEIVNDYQLGNWDDYKFYENFNFAYTLFREEKCDAVFEEIKQNKFGGKNLSFHEYLNEYAHRSKESDMLAPIDYFIDQNNLNNVFDRQKHAIYKLYLLDLILNELCRSMNNDHRINNEFKYFDCEVIYVEDLYLKILYKLYFQLN" }, "paralog": false, "identity": 0.985781990521327, "score": 421.0, "evalue": 2.96e-152, "description": "Internal stop codon at 99197. Nonsense mutation around 99197.", "psc": { "uniref90_id": "UniRef90_Q9PR84", "product": "Uncharacterized protein UU061", "uniref50_id": "UniRef50_Q9PR84", "go_ids": [ "GO:0016020" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PR84", "UniRef:UniRef50_Q9PR84", "GO:0016020" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PR84", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR84" ], "product": "Uncharacterized protein UU061" } }, "id": "NKIECMJPJD_185", "locus": "GCA000828735_00149" }, { "type": "cds", "sequence": "contig_1", "start": 99587, "stop": 100303, "strand": "-", "frame": 2, "gene": null, "product": "Mitochondrial potassium channel ATP-binding subunit", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A6J7TFN9" ], "nt": "GTGCGTTTTGAAAATGTTAATTTTAATTATTTAAAAAATACGCCTACTTTAAAAAATATTAATTTTGTTGCCAAAAAGAACCAAAGAATTGCTATTATTGGACCCACAGGTGCTGGAAAAACAACAATTACTAACTTAATTAATAAATTTTATGATATTAATAATGGCTTTATTTATTTTAACGATGTTGACATTACAAATAAATCCCGTGAAAGTGTGCGTGAACATATTTCTATTGTTTTACAAGATCCTTTTTTATTTAGTGAAAGTATTTATGAGAATATCAAAAAAGGTAAAATGAATGCAACAAAAGAAGAAATTATTGCAGCAGCTAAAAAAGCTCAAGTTCATGATTTGATCCTTTCATTTCCAAAGGATTATGCTACAGTTATTGATGAAAAACAAAACTTATCACAAGGTCAAAAACAATTAATAACTATCGCTCGTGCTATTATTTCTGATGCGAAAATTATTATTTTAGACGAAGCAACAAGCTCTGTGGATACGCAAACCGAACATAAATTACAATTAGCAATTAATAATTTACTAAAAGGACGAACATCATTTATTGTTGCCCATCGATTATCAACAATTATTAACTCTGATTTAATTCTTGTAATTAAAGATGGTGAAATTATTGCACAAGGGAATCATGATTATTTGATTAAGAATTCGCCTTTTTATCAAGAACTTTACTATGCAAATTTTAATGAATAA", "aa": "MRFENVNFNYLKNTPTLKNINFVAKKNQRIAIIGPTGAGKTTITNLINKFYDINNGFIYFNDVDITNKSRESVREHISIVLQDPFLFSESIYENIKKGKMNATKEEIIAAAKKAQVHDLILSFPKDYATVIDEKQNLSQGQKQLITIARAIISDAKIIILDEATSSVDTQTEHKLQLAINNLLKGRTSFIVAHRLSTIINSDLILVIKDGEIIAQGNHDYLIKNSPFYQELYYANFNE", "aa_hexdigest": "9e5f33d9b5c2d765e4c8ec11210c1421", "start_type": "GTG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI003217A8C9", "query_cov": 0.9873949579831933, "subject_cov": 0.8545454545454545, "identity": 0.523, "score": 241.0, "evalue": 1.92e-76, "valid": false, "product": "ABC transporter ATP-binding protein", "uniref50_id": "UniRef50_A0A6J7TFN9" }, "pscc": { "uniref50_id": "UniRef50_A0A6J7TFN9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A6J7TFN9" ], "product": "Mitochondrial potassium channel ATP-binding subunit" }, "genes": [], "id": "NKIECMJPJD_186", "locus": "GCA000828735_00150" }, { "type": "cds", "sequence": "contig_1", "start": 100343, "stop": 100603, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCGATTAATGAATGCCGTTTTAATTGTGTCTTATTTATTAATTTCTATTAAAGGAATTCATTTGCCAGGGCTTATAAATAATATTGATCCAACAACGAATATTATTTCTTTTGGTGGTTTAGTTAGTATTTCATTATTTGGTAATTTCTTTTGTGATAATTTTAGTCAATTATCAAATACTATTCCTATTTTTATCGTTGCTAGAACATCACTTGCTAAAATTGATGAAATTGTTAAAACTCCTAATGAGATAAATTGA", "aa": "MRLMNAVLIVSYLLISIKGIHLPGLINNIDPTTNIISFGGLVSISLFGNFFCDNFSQLSNTIPIFIVARTSLAKIDEIVKTPNEIN", "aa_hexdigest": "2c1b263ad88acae696c294dacf58a17d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9436.02049999999, "isoelectric_point": 6.518375205993652 }, "id": "NKIECMJPJD_187", "locus": "GCA000828735_00151" }, { "type": "cds", "sequence": "contig_1", "start": 100655, "stop": 101179, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTGTGCAGGATTGATAGGAATTTATTTTTTCTCATTTATATTTTTACTAATTATCAACGAATTTGCCATTCGAATATCTTTTAAGATTGAATCCCATTTATCAATGATGGTGATTAATCGTATTCGTTATTTACCAATGAAGTATTTTGATATTAATAAAAGCGGAGAAATTTTTACTAAAACACTAAGTGATCCAACAAGTGTACAAGATGGAATTGTTAATTTTTATATTGAGTTAAATAAAGCTATATTTGGCGCAATTGGTTTTGGAACTGCTTTATTAATAACAAGTCCTTATATTGCTTTAATTGGAATTGTCATTTATGTTCTAGTAATGCTATTTAATATTTTGATATTTAAAAAATCCCATCAATTAATGCGCATAAAACGGCAAAATTTTGGTGAAATGAACGGATATATTGAAGAAATGATTTATGGGCAAAACGTTGTAGCTAATTTTAATGAACAAGTTTTTTTCATAAAAAAATTAGATAATATGATTAAAAAATTATATAAAAATTGA", "aa": "MCAGLIGIYFFSFIFLLIINEFAIRISFKIESHLSMMVINRIRYLPMKYFDINKSGEIFTKTLSDPTSVQDGIVNFYIELNKAIFGAIGFGTALLITSPYIALIGIVIYVLVMLFNILIFKKSHQLMRIKRQNFGEMNGYIEEMIYGQNVVANFNEQVFFIKKLDNMIKKLYKN", "aa_hexdigest": "64a0ad80698c2573da5aeaf4ab42fef0", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 20187.907799999994, "isoelectric_point": 9.38364734649658 }, "id": "NKIECMJPJD_188", "locus": "GCA000828735_00152" }, { "type": "cds", "sequence": "contig_1", "start": 101270, "stop": 101383, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGCCAACAACTACAAACCAATAAACCTGTAAGTAAACTTTATTTAATTTATTATTATTTTAAGGATTTTAAAAAAGTATTTATTGGTTTGTCAATTGCCATTATTTTTTGA", "aa": "MSQQLQTNKPVSKLYLIYYYFKDFKKVFIGLSIAIIF", "aa_hexdigest": "2d3e448ff3697656ca7f55c7408d5c80", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4449.298999999999, "isoelectric_point": 9.69941349029541 }, "id": "NKIECMJPJD_189", "locus": "GCA000828735_00153" }, { "type": "cds", "sequence": "contig_1", "start": 101358, "stop": 101969, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00005.33" ], "nt": "ATGCATCAATTAGAACCAAATAGCGGAGAAATATTTATTGATAATATTCCTTTAAAAGATATTAAAAAAACAAGTTTGCAAGCTGCTTTTAGCCTTAATGAACAAGTCCCTTTGCTTTTTTATGGAACTATCCGTGATAACGTATTATTTGGTTTAGAGGATATTGATGAAAACAAAATCAACGAAGTTATTGAAATTGCACAAGCTAAGGATTTTATTGAATCTAAGAATGAAAAATTAGAATCTCTAATAGCACAAAGGGCTCAAAATCTATCTGGAGGACAAAAACAACGTCTAGCAATGGCACGTGCATTGTTGCGCGACGCAAGAACTTTAGTGCTAGATGATTCAACTAGCGCATTAGACATGATCACTGAAAAAAAGTTTTTAACTGCACTAAAACAACAAATTAAATTTAAGAATTCGATTATTATTGCTCAAAGAATTAATAATATTTTAGACTGTGACAAAATTATTGTAATGGATAAAGGTAAGATTGTTGGAATTGGTAATAATGAGACATTACTTAAAACTTGTGCAGTTTATAAAAATATCTATCAACATCAAATAAATAAAGGAGCAGAATATGAGCCAACAACTACAAACCAATAA", "aa": "MHQLEPNSGEIFIDNIPLKDIKKTSLQAAFSLNEQVPLLFYGTIRDNVLFGLEDIDENKINEVIEIAQAKDFIESKNEKLESLIAQRAQNLSGGQKQRLAMARALLRDARTLVLDDSTSALDMITEKKFLTALKQQIKFKNSIIIAQRINNILDCDKIIVMDKGKIVGIGNNETLLKTCAVYKNIYQHQINKGAEYEPTTTNQ", "aa_hexdigest": "9b03054764815099a35ca9552f6958e7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00005.33", "name": "ABC_tran", "length": 119, "aa_cov": 0.5812807881773399, "hmm_cov": 0.8823529411764706, "evalue": 1.4847980458929031e-15, "score": 53.59562301635742, "start": 2, "stop": 119 } ], "seq_stats": { "molecular_weight": 22921.125500000016, "isoelectric_point": 6.175238227844238 }, "id": "NKIECMJPJD_190", "locus": "GCA000828735_00154" }, { "type": "cds", "sequence": "contig_1", "start": 102066, "stop": 102299, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGCAACAAACATTTTATCAATCATGCAATCAACTAATTTATTAGTTTTAGGAATTGCGTTAATTGGTTTTGGAAGCGAACAGTATTTTAGATCTATAGTAAGTATTAAAAGAATTAATGATTTAATTTTTTATAAAAATCAAATTGTTTATAATGGTACAAAAGAAATTAATAACAATAATATTGAATTCAAAAATGTAAGTTTTAGGTATCATAATTCAAAAAAATGA", "aa": "MKATNILSIMQSTNLLVLGIALIGFGSEQYFRSIVSIKRINDLIFYKNQIVYNGTKEINNNNIEFKNVSFRYHNSKK", "aa_hexdigest": "4a736d690495bbe4eaf80b35ca1ee336", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8917.234199999995, "isoelectric_point": 9.917123222351073 }, "id": "NKIECMJPJD_191", "locus": "GCA000828735_00155" }, { "type": "cds", "sequence": "contig_1", "start": 102349, "stop": 102681, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATAAAAGTAACAATAGCTATGCTTACTTTACTAATTCGATTGGCAATAATAGATTTAGAAACATTATCTTTTCATTTAAAATCTAATTTTGTTAAAAAAAATGCATCTAATAAGAAAGAACGAATAACTTTTACTCCACTAACATTTTCTAATGACACTTTTGCTAATTCATCATTAGCAACACGCATTTTATTTCAATTTTTAGCATTAAATTTTGAAATAATCATAAAAATAATAATTGTTGATAAAGCCATCAAAACTACAGTTAAAGGGATTTGTCATAAATTACCACTAATAATGAATAATAATATAATTGCTCCAATGAAATAA", "aa": "MIKVTIAMLTLLIRLAIIDLETLSFHLKSNFVKKNASNKKERITFTPLTFSNDTFANSSLATRILFQFLALNFEIIIKIIIVDKAIKTTVKGICHKLPLIMNNNIIAPMK", "aa_hexdigest": "42f3aa718d3140b15216177aca694483", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12459.982799999982, "isoelectric_point": 10.149854087829588 }, "id": "NKIECMJPJD_192", "locus": "GCA000828735_00156" }, { "type": "cds", "sequence": "contig_1", "start": 102630, "stop": 102809, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTCTTAACATATGCAGATAAACAAAAATTTAATCAATCAATTTTATTAACAACTTCAATAAAAGACGTCGAAGAACTTTCAAAAACAATTATCTTTTTAACACGACCTGTAATTATGGGAATTTTTTATTTCATTGGAGCAATTATATTATTATTCATTATTAGTGGTAATTTATGA", "aa": "MFLTYADKQKFNQSILLTTSIKDVEELSKTIIFLTRPVIMGIFYFIGAIILLFIISGNL", "aa_hexdigest": "74790b5724ad9c75d59afe43aaa35c1d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6744.047799999995, "isoelectric_point": 8.09853458404541 }, "id": "NKIECMJPJD_193", "locus": "GCA000828735_00157" }, { "type": "cds", "sequence": "contig_1", "start": 102900, "stop": 103076, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTAATAAAACATAAATTTTTATTTTTCTTTATTTTATTTTTAAATTTAGTACAAGTTTTATTAGTAATTGGTTCTCCTCTTTTATTATCAATTCTTAATAATGGTAATTATGCATCAACAACCGCTGACGCTTTTATTTATGGCGGTGTGTTAATCGCCATGGCTATAGGATGA", "aa": "MLIKHKFLFFFILFLNLVQVLLVIGSPLLLSILNNGNYASTTADAFIYGGVLIAMAIG", "aa_hexdigest": "786b25340e8d71559d049fce064e74cd", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6330.626700000001, "isoelectric_point": 8.210645103454592 }, "id": "NKIECMJPJD_194", "locus": "GCA000828735_00158" }, { "type": "cds", "sequence": "contig_1", "start": 103117, "stop": 103542, "strand": "-", "frame": 3, "gene": "smpB", "product": "SsrA-binding protein SmpB", "db_xrefs": [ "COG:COG0691", "COG:O", "GO:0003723", "GO:0005829", "GO:0070929", "KEGG:K03664", "RefSeq:WP_006688578.1", "SO:0001217", "UniParc:UPI0000136037", "UniRef:UniRef100_Q9PR87", "UniRef:UniRef50_Q9PR87", "UniRef:UniRef90_Q9PR87" ], "nt": "ATGATTGTATCAAATAAACACGCACGTCGAAATTATGAATTACTAGATTTTTTTGAATGTGGAATCGTTTTAAAAGGAACTGAAGTTAAATCTATTTCACGTGCTAATTGCTCAATTAATGAAGCTTATGTCCAAATAATTAAAAATCAAGCTTTAATTTTAAATATGCATGTTGCAAATTTTTTTGAGGGTAATAACTTTAATCAAGATCCATACCGGAATCGAAAACTATTATTACACAAAAAAGAAATTATTAGACTTCAACATTTAGTGAAAACACAACATATGACAATTGTGCCAACCAAAATTTATTGGAAAAATAATAAACTAAAGATAGAGATTGCTTTAGGAAAAGGTAAACAGCTTCATGATAAACGTGAGGATATAAAAAAAAGAGATTTAGCGCGTGAAACGCGTTTATTTTAA", "aa": "MIVSNKHARRNYELLDFFECGIVLKGTEVKSISRANCSINEAYVQIIKNQALILNMHVANFFEGNNFNQDPYRNRKLLLHKKEIIRLQHLVKTQHMTIVPTKIYWKNNKLKIEIALGKGKQLHDKREDIKKRDLARETRLF", "aa_hexdigest": "86048fe7fb3a657f4dcce40194de455f", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000136037", "ncbi_nrp_id": "WP_006688578.1", "uniref100_id": "UniRef100_Q9PR87", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000136037", "RefSeq:WP_006688578.1", "UniRef:UniRef100_Q9PR87" ] }, "ips": { "uniref100_id": "UniRef100_Q9PR87", "uniref90_id": "UniRef90_Q9PR87" }, "psc": { "uniref90_id": "UniRef90_Q9PR87", "gene": "smpB", "product": "SsrA-binding protein SmpB", "uniref50_id": "UniRef50_Q9PR87", "cog_id": "COG0691", "cog_category": "O", "kegg_orthology_id": "K03664", "go_ids": [ "GO:0003723", "GO:0005829", "GO:0070929" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PR87", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR87" ], "product": "SsrA-binding protein" }, "genes": [ "smpB" ], "id": "NKIECMJPJD_195", "locus": "GCA000828735_00159" }, { "type": "cds", "sequence": "contig_1", "start": 103742, "stop": 104158, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00575.29" ], "nt": "ATGTTTATTTTTGATTCTCAAAGTTATACAGATAAACAACGAGTAGAATTAGTAAATAAACTGAAGTTAATTACTGAAGAATCAAATAAAAATGAAATTATAGCAGTTAAGACTGAACGTGATGTTAATGCTGCTAAATTTGCTGAATATATGAATTTACATATTGGTAAAGAATTTATTGGTGTGGTAACAACAGTTTCTAGTTTTGGTGTTTTTGTTGAACTCGAAAATACGATTGAAGGTTTAATTAGAATTAAAAATTTAAAAGATGATTTTTATGATTTTATTCCTGAAAACATGACTTTAGTTGGTCAAAAAAGAAAGAAAATTATTACTGTTGGCAATAAAGTTCGTGTACGTGTTATTGAAGCTAACAAACTTACAAGAAAAATTGATTTTGAACTAGTTGCACAATAA", "aa": "MFIFDSQSYTDKQRVELVNKLKLITEESNKNEIIAVKTERDVNAAKFAEYMNLHIGKEFIGVVTTVSSFGVFVELENTIEGLIRIKNLKDDFYDFIPENMTLVGQKRKKIITVGNKVRVRVIEANKLTRKIDFELVAQ", "aa_hexdigest": "ce78b5a220f0755ae06e9358bab16193", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00575.29", "name": "S1", "length": 80, "aa_cov": 0.5797101449275363, "hmm_cov": 0.8625, "evalue": 3.8287613248776476e-14, "score": 48.44105911254883, "start": 55, "stop": 134 } ], "seq_stats": { "molecular_weight": 15930.30679999999, "isoelectric_point": 8.776226234436034 }, "id": "NKIECMJPJD_196", "locus": "GCA000828735_00160" }, { "type": "cds", "sequence": "contig_1", "start": 104159, "stop": 104335, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00773.24" ], "nt": "ATGAATGATAATCATGTTAACTTACCATTAATTAGTAAATTATTATTAAGATCAATGGCTAAAGCTTCTTATGAAATTATTAATACGGGACATTTTGGTTTAGCATCTGATAACTATACACACTTTACATCACCAATACGGCGTTATCCAGATTTAATTGTACACCGTTTATTATGA", "aa": "MNDNHVNLPLISKLLLRSMAKASYEIINTGHFGLASDNYTHFTSPIRRYPDLIVHRLL", "aa_hexdigest": "6971f96e19a9ce6d74715c3be36ab6b2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00773.24", "name": "RNB", "length": 55, "aa_cov": 0.9482758620689655, "hmm_cov": 1.0, "evalue": 1.6230804366329469e-24, "score": 82.2816162109375, "start": 4, "stop": 58 } ], "seq_stats": { "molecular_weight": 6678.656799999999, "isoelectric_point": 9.395573997497557 }, "id": "NKIECMJPJD_197", "locus": "GCA000828735_00161" }, { "type": "cds", "sequence": "contig_1", "start": 104342, "stop": 104818, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00773.24" ], "nt": "ATGAAAAATTATCGTCAAATGTCATATGATGAAGTTAATGATTTTTATGAAGGAAAATCACGTTTTGAATCAGCAACATTAACTATGAAAGAAATGTTATTAGAAGCTAAAGAATTGCACCATATTTTAAGAAATAAAAAAATTAAAGATGGTTATGTTGATTTTGATATTAAAGAACCAAAAATTATTTTAGATGAAACTGGTGTCCCTATTGATATTAAAATTTACGAACGTAAAACTGCTCAAATGATGGTTGAAGATTTTATGATTGCAGCTAATGAGGCTGTAACTATGTTTGCTGAAGAACACATGGATAAAACACTAAAAGAATTTAATTTAGAAATGCCTTTTATCTATCGTGTTCATGATAAACCATCAATTATTAACTTACAAAAATTTGAAATTGAGGCAAAAAAATTGAGTTTCAATATCTCACATGATTTTGAGAACATTCAACCTAACACTATTTCAAATTGA", "aa": "MKNYRQMSYDEVNDFYEGKSRFESATLTMKEMLLEAKELHHILRNKKIKDGYVDFDIKEPKIILDETGVPIDIKIYERKTAQMMVEDFMIAANEAVTMFAEEHMDKTLKEFNLEMPFIYRVHDKPSIINLQKFEIEAKKLSFNISHDFENIQPNTISN", "aa_hexdigest": "77ac5994b74e8bdc545ce37bb204e193", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00773.24", "name": "RNB", "length": 161, "aa_cov": 0.9683544303797469, "hmm_cov": 0.9316770186335404, "evalue": 3.763772607198682e-30, "score": 100.7998275756836, "start": 1, "stop": 153 } ], "seq_stats": { "molecular_weight": 18749.36790000001, "isoelectric_point": 5.292926597595215 }, "id": "NKIECMJPJD_198", "locus": "GCA000828735_00162" }, { "type": "cds", "sequence": "contig_1", "start": 104840, "stop": 105907, "strand": "-", "frame": 2, "gene": "rnr", "product": "ribonuclease R", "db_xrefs": [ "COG:COG0557", "COG:K", "EC:3.1.13.1", "GO:0003723", "GO:0004518", "GO:0005829", "GO:0006402", "GO:0008859", "KEGG:K12573", "SO:0001217", "UniRef:UniRef50_Q9PR88", "UniRef:UniRef90_Q9PR88" ], "nt": "ATGAGTGATTTTACGAAACAAACTATTACCGAAGTAATTTCTAAGGAAGAGCGCCCAATACCCGCTGCTATTCTTGCTAAAAAAGTGTTAGAAAAAATACCCACTTTAAATAAAACTGATGTTTATAAACTAATTGATTTATTAATTCAAGAAAATACTATTAAAAAATTAGAAAATAATCGATTGGTTATTGGTTATCTAGATTATGAATTTGATCACGAGATCAAACAAGGAATAATTACTATCAATTCAAAAGGTGATGGCTTCATTAAAGAAGATAATACTGAAATTGAATATTACGTCAACAAAAAATATTTAAATGGGGCTTTAAAAAAAGATTCAGTTAAATTTGTAAAGTTAAAAAAAGAACCTAAAAACAACCTGCAAGATGCTGCTGTAATTGAAATTGTTGGGCATGCTAAAGACCATTATGTTGGTCAATTTATAACTCTTCCTAATGGTGGTTATTACATTTTTGTTGACGATCCTTTATTTTACTTAAATATAAATTTAAAAGATACTACTGGTTTAGTTAATGGTCACAAAATTCTTTTTAAAATTATTTCGCAAACAACAAAAGACGCTATTGCAGAATTAGTTCATATCATAGGTCATAAAAACGATGTTGGTTCTGATGTTTTATCAATCGTTTATGATAATGGTATTGATCCGACTTTTGATCCACAAGTTGTTGATTTGGCTTCAAAATTAGAATTTTATGTTGATGAACATCAAAATAAAATTCGACGTTCAATAATTGATCGTGAAATTATTTCTATTGATCCTGTTGGTTCTAAAGATATTGATGATGCTGTTTATGTTAAAAAATTAAATGATCAACGTTACTTTTTAGGTATTTCTATTGCTGATGTTAGTTTTTATGTACAACCTAACACCATTCTTGATGCTGATGCTTTTAAAAGGGGAACATCAACATATTTAGTTGATCGTGTTATTCCAATGCTTCCGCATAATATTTCTAATAATATTTGTTCATTAAACGAAGGTGAGTTTCGCATGTGTATAACATGTGATATGGTAATTGACAAAGATGGAAAAATATGTTGA", "aa": "MSDFTKQTITEVISKEERPIPAAILAKKVLEKIPTLNKTDVYKLIDLLIQENTIKKLENNRLVIGYLDYEFDHEIKQGIITINSKGDGFIKEDNTEIEYYVNKKYLNGALKKDSVKFVKLKKEPKNNLQDAAVIEIVGHAKDHYVGQFITLPNGGYYIFVDDPLFYLNINLKDTTGLVNGHKILFKIISQTTKDAIAELVHIIGHKNDVGSDVLSIVYDNGIDPTFDPQVVDLASKLEFYVDEHQNKIRRSIIDREIISIDPVGSKDIDDAVYVKKLNDQRYFLGISIADVSFYVQPNTILDADAFKRGTSTYLVDRVIPMLPHNISNNICSLNEGEFRMCITCDMVIDKDGKIC", "aa_hexdigest": "bae008414f5969d98d1a9b341a1fa5ec", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "rnr" ], "truncated": "5-prime", "pseudogene": { "start": 104537, "stop": 105907, "observations": { "mutation": [ 104842 ], "stop": [ 104842 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PR88", "query_cov": 1.0, "subject_cov": 0.492371705963939, "identity": 1.0, "score": 701.0, "evalue": 1.91e-249, "gene_start": 1, "gene_end": 355, "reference_start": 1, "reference_end": 355, "reference_sequence": "MSDFTKQTITEVISKEERPIPAAILAKKVLEKIPTLNKTDVYKLIDLLIQENTIKKLENNRLVIGYLDYEFDHEIKQGIITINSKGDGFIKEDNTEIEYYVNKKYLNGALKKDSVKFVKLKKEPKNNLQDAAVIEIVGHAKDHYVGQFITLPNGGYYIFVDDPLFYLNINLKDTTGLVNGHKILFKIISQTTKDAIAELVHIIGHKNDVGSDVLSIVYDNGIDPTFDPQVVDLASKLEFYVDEHQNKIRRSIIDREIISIDPVGSKDIDDAVYVKKLNDQRYFLGISIADVSFYVQPNTILDADAFKRGTSTYLVDRVIPMLPHNISNNICSLNEGEFRMCITCDMVIDKDGKICWKDVYPAIMKNYRQMSYDEVNDFYEGKSRFESATLTMKEMLLEAKELHHILRNKKIKDGYVDFDIKEPKIILDETGVPIDIKIYERKTAQMMVEDFMIAANEAVTMFAEEHMDKTLKEFNLEMPFIYRVHDKPSIINLQKFEIEAKKLSFNISHDFENIQPNTISNWLKMNDNHVNLPLISKLLLRSMAKASYEIINTGHFGLASDNYTHFTSPIRRYPDLIVHRLLWMFIFDSQSYTDKQRVELVNKLKLITEESNKNEIIAVKTERDVNAAKFAEYMNLHIGKEFIGVVTTVSSFGVFVELENTIEGLIRIKNLKDDFYDFIPENMTLVGQKRKKIITVGNKVRVRVIEANKLTRKIDFELVAQ" }, "paralog": false, "identity": 0.9978070175438597, "score": 906.0, "evalue": 0.0, "description": "Internal stop codon at 104842. Nonsense mutation around 104842.", "psc": { "uniref90_id": "UniRef90_Q9PR88", "gene": "rnr", "product": "ribonuclease R", "ec_ids": [ "3.1.13.1" ], "uniref50_id": "UniRef50_Q9PR88", "cog_id": "COG0557", "cog_category": "K", "kegg_orthology_id": "K12573", "go_ids": [ "GO:0003723", "GO:0004518", "GO:0005829", "GO:0006402", "GO:0008859" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PR88", "EC:3.1.13.1", "UniRef:UniRef50_Q9PR88", "COG:COG0557", "COG:K", "KEGG:K12573", "GO:0003723", "GO:0004518", "GO:0005829", "GO:0006402", "GO:0008859" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PR88", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR88" ], "product": "Ribonuclease R" } }, "id": "NKIECMJPJD_199", "locus": "GCA000828735_00163" }, { "type": "cds", "sequence": "contig_1", "start": 105986, "stop": 106132, "strand": "-", "frame": 2, "gene": "secG", "product": "preprotein translocase subunit SecG", "db_xrefs": [ "GO:0005886", "GO:0009306", "GO:0015136", "GO:0015450", "GO:0015739", "GO:0016020", "SO:0001217", "UniRef:UniRef50_P58061", "UniRef:UniRef90_Q9PR89" ], "nt": "ATGGCATTAACAATTGTTTTAATCTTGTTTAGTGTTCTAGCTTTAATAATAGGTTTATTATTATCAAGAACTTCACCATCTGGTGGATTGTCAAGTTTAAATGGACAAGATCTAGAAATTTTTAAAAAAACAAAAGACCGTGGTTGA", "aa": "MALTIVLILFSVLALIIGLLLSRTSPSGGLSSLNGQDLEIFKKTKDRG", "aa_hexdigest": "71e74320b93577c3a7cc384bef79344a", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [ "secG" ], "truncated": "5-prime", "pseudogene": { "start": 105908, "stop": 106132, "observations": { "mutation": [ 105988 ], "stop": [ 105988 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PR89", "query_cov": 1.0, "subject_cov": 0.6486486486486487, "identity": 1.0, "score": 85.5, "evalue": 5.97e-21, "gene_start": 1, "gene_end": 48, "reference_start": 1, "reference_end": 48, "reference_sequence": "MALTIVLILFSVLALIIGLLLSRTSPSGGLSSLNGQDLEIFKKTKDRGWIKGLQVFMFLLTIVMILIIIFYRVS" }, "paralog": false, "identity": 0.9864864864864865, "score": 135.0, "evalue": 3.38e-43, "description": "Internal stop codon at 105988. Nonsense mutation around 105988.", "psc": { "uniref90_id": "UniRef90_Q9PR89", "gene": "secG", "product": "preprotein translocase subunit SecG", "uniref50_id": "UniRef50_P58061", "go_ids": [ "GO:0005886", "GO:0009306", "GO:0015136", "GO:0015450", "GO:0015739", "GO:0016020" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PR89", "UniRef:UniRef50_P58061", "GO:0005886", "GO:0009306", "GO:0015136", "GO:0015450", "GO:0015739", "GO:0016020" ] }, "pscc": { "uniref50_id": "UniRef50_P58061", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P58061" ], "product": "Probable protein-export membrane protein SecG" } }, "id": "NKIECMJPJD_200", "locus": "GCA000828735_00164" }, { "type": "cds", "sequence": "contig_1", "start": 106227, "stop": 106343, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCGTAGTTTATACCCTAATGAATATCATGATAAATTATTTTATATTCGTAACGATATTTCTGATCATGCACCTACATTCTTTGATTTAGGTTTAAATAAAAACGACACTAAATAA", "aa": "MRSLYPNEYHDKLFYIRNDISDHAPTFFDLGLNKNDTK", "aa_hexdigest": "73625463e38a040807382eb00e526749", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4590.048200000001, "isoelectric_point": 5.981758308410644 }, "id": "NKIECMJPJD_201", "locus": "GCA000828735_00165" }, { "type": "cds", "sequence": "contig_1", "start": 106557, "stop": 107138, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATAGGAATCATTAAGTATTTAAATAAGTTAAGTGGCTCAGATGATTATGATTTTTTAATTAGTGATAATCTTAAAGGTAGTGAAAATGATAAATTAGCAACAAACGAACATACAAGTACTGAACGAATTGGGATAATTTATAATAAGAAAAAAGTTAAACCTATTCCGTTTGCAAATAATAAAATCGGTTATATTTATTCAAATCCATTACAACCTGGCAAATATGAAAAAGCAGAAACTGATTATTCACGTCCACCATTTGCTATTAAATTTGCTTCAATTGGTCAAATTAAAAATGATTTTACATTGGTTTTTGGTCATCTTGATAGTCCTGGAACTTATCCATCAAATACAGAATATCAAAAGAAAGAGCATCTACTAAAACGTACAGAATTAGTTAATAAAAAAGTAATTTCAACGAACCAAATGATTAATAAATATGAACAAGGAAGTCGTGAAGTGGACGATGCTGAACGATTAGTCAAAGTTATGGAAGAAATTAAAAAAATTGATAACAATAAGGATGATGATTATTTCTTTTTAGGTGATACTAATATTCGTTTACATAATCAACAATGA", "aa": "MIGIIKYLNKLSGSDDYDFLISDNLKGSENDKLATNEHTSTERIGIIYNKKKVKPIPFANNKIGYIYSNPLQPGKYEKAETDYSRPPFAIKFASIGQIKNDFTLVFGHLDSPGTYPSNTEYQKKEHLLKRTELVNKKVISTNQMINKYEQGSREVDDAERLVKVMEEIKKIDNNKDDDYFFLGDTNIRLHNQQ", "aa_hexdigest": "51704fd772184a11d46957566e20802f", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 22260.85490000001, "isoelectric_point": 7.790633583068848 }, "id": "NKIECMJPJD_202", "locus": "GCA000828735_00166" }, { "type": "cds", "sequence": "contig_1", "start": 107280, "stop": 109310, "strand": "-", "frame": 1, "gene": null, "product": "Membrane nuclease A-hypothetical", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B5ZAN4", "UniRef:UniRef90_Q9PR90" ], "nt": "GTGGATAAAAAATTTGTTTTTGACAGTTTAGATGAGAATGTTAAATATCTACTAAAACAAATTGATTTTTACGATCCAAAAGCGCCTAATCAAATTATTTATACAAAAAACAATCAGACATTTAGTTTTGAAACTAAAATGGATGATAGTATCGCTAGAACTGTTGAACTAATTATTATTAGTGGGTTTAATGGTTTTGTTGATGAACAAATTGTTCCAGATTATAATAAACAAACTACATATTTAAAAGCACCCGGTTTAATTCGATTATTAAATGAAGTTAATAAAATTAAAAAAGAACGAGGTGCGAATAATGTTGCTGTAGTTATGGGTGGAAATAATTATTATGAAAATAATATATCTTCATTAACTAGAGCTCAATATACAACAGCCGCATTAAAGTTTTTAAATCCAGTAGCTACTAGCATCGCTAATACTGATTTAAATTGGGGTTATGATTTAATTGATGAAAAAACAAAGCATTTTAAAGAATACGTCAAAGCTTTATATGGTAATGATGGTATTTTATCTGTTAACTCTTATCTAAAAGATGGTGATAAAAACATACAAATGACTAATAATTCAAAAATTATTAACTTTGGTGGTTTTAAATTAGGATTAATTGGAGCAATGAGTAATTTAATTAATTTTGAATCTAATCTTAATTTAACAAAAAATATTCAATGGTTTAGTAACGAACAAACAGCTAATTTGATTAATGATGAAGCCAATAAGCTTAAGGGTCAAGGTGTTAAAAATATTGTTTTATTATCAAGTTTTTTAGCAGATAAAGCAAAGGAAAATAACACTTTAACTAATGATGAAAAATCTTTTTATAAAGAAGCAACACGAATGAGTAAATTAATAGATAATAATGTCAATTTAATGAGTGTTATTAATCCTTACAAAGCACTTAATGTAATTGCTAATAATAAAAATAATCAACCTTTGAATGTTGTTCAATCAAAACCTAATAGTGGTTTTGTACGATATATGATCACACTAAATCCTTATAACCGTATTTTAAAAACAGAAATTAAGCTTGTTGATAAACTGTCAACAAGCGGTGATAATGCAGGATATCATTATATAACTCAACAAGATTTATTAAATCCACAAAAAAATCCTGATTTCAATCAAAATTTGATTACATTAAATAATTTTCTTAATAATTATAAAGATCATGAATACGAGCAATTAAGAGAAAAAATACTGATTAAAAATGATAAAAAAGTCAATTTATATTCTTCAACATATTCACTTAGTTCCTTAGGAGCGTTTATAAGTGATATATTAGCAAAATACTATCAAGCTCCTTTTTATAATAAACAAACACATACTTTTGAATTTAAACAAACGCAAGCTATCCTATTAGCTCCTCATGCTATTCGTTCTTTGGTTTCTGCTAAACCTATTAAACTAGAAAATTTAAATCAAATTTTACCTTTTAATGATACGTTAGTGTCAGGAGAAATTAGTGTTAAAAATCTTAAAGAATTTTTAATTAACAAGAATAAATCTCAAAATTACAACGAACCATATCAATTTAGTAATACGCTAAAAATTGAATATGAAAACTTACAAGAAACAAAAACAAAGAAAAAATCAAGAAAAAAACAATCAGAACAAAGTTATAGTGATAATGAAAAAAAAGATGGTCTAATTTTAAGTCATCAAAAAAGAGTTATTAAAGTCTTTTTGAAAAATTCAGATGGTACGTATACAGAATTAAATGATAATCAAAAAATTATAATAAGTGTTATGAATTCGATCTTAAAATTTAATAAAAAATTTAGTGAGGCTCTTTTAAATAAAACATACGCTGACCAAACTAATATTCGTGATTTACTAAGAACATACTTACCAAAAGAAACACAAATCGATCAAAAATATGATCAAAGTGTGTTTAGTAAAAATGATGATCGTTTTTATGAAAAAAATGAAACAGACCAAAATAAATCACTTATTTACAATCATATTAATGAAAGTATTAATAATAAACAAGCCGATCATTTAAGAATTGGGCACTGA", "aa": "MDKKFVFDSLDENVKYLLKQIDFYDPKAPNQIIYTKNNQTFSFETKMDDSIARTVELIIISGFNGFVDEQIVPDYNKQTTYLKAPGLIRLLNEVNKIKKERGANNVAVVMGGNNYYENNISSLTRAQYTTAALKFLNPVATSIANTDLNWGYDLIDEKTKHFKEYVKALYGNDGILSVNSYLKDGDKNIQMTNNSKIINFGGFKLGLIGAMSNLINFESNLNLTKNIQWFSNEQTANLINDEANKLKGQGVKNIVLLSSFLADKAKENNTLTNDEKSFYKEATRMSKLIDNNVNLMSVINPYKALNVIANNKNNQPLNVVQSKPNSGFVRYMITLNPYNRILKTEIKLVDKLSTSGDNAGYHYITQQDLLNPQKNPDFNQNLITLNNFLNNYKDHEYEQLREKILIKNDKKVNLYSSTYSLSSLGAFISDILAKYYQAPFYNKQTHTFEFKQTQAILLAPHAIRSLVSAKPIKLENLNQILPFNDTLVSGEISVKNLKEFLINKNKSQNYNEPYQFSNTLKIEYENLQETKTKKKSRKKQSEQSYSDNEKKDGLILSHQKRVIKVFLKNSDGTYTELNDNQKIIISVMNSILKFNKKFSEALLNKTYADQTNIRDLLRTYLPKETQIDQKYDQSVFSKNDDRFYEKNETDQNKSLIYNHINESINNKQADHLRIGH", "aa_hexdigest": "4e36ef42b9dd5d18ba7ce4955e698952", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 106977, "stop": 109610, "observations": { "mutation": [ 107282, 109346 ], "stop": [ 107282, 109346 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PR90", "query_cov": 1.0, "subject_cov": 0.5940246045694201, "identity": 0.996, "score": 1298.0, "evalue": 0.0, "gene_start": 1, "gene_end": 676, "reference_start": 112, "reference_end": 787, "reference_sequence": "MVCKKNKLKMFGFIAIGTVLTSAIIPFLVTCAKEKKIKQEVIKPKQISNLNLEAKNIDIVLKNNVFNKETAKFTLDFDINKELNLQMVAVFDVFDKKNNWLKTIKTAKTNLVDKKFVFDSLDENVKYLLKQIDFYDPKTPNQIIYTKNNQTFSFETKMDDSIARTVELIIISGFNGFVDEQIVPDYNKQTTYLKAPGLIRLLNEVNKIKKERGANNVAVVMGGNNYYENNISSLTRAQYTTAALKFLNPVATSIANTDLNWGYDLIDEKTKHFKEYVKALYGNDGILSVNSYLKDGDKNIQMTNNSKIINFGGFKLGLIGAMSNLINFESNLNLTKNIQWFSNEQTANLINDEANKLKGQGVKNIVLLSSFLADKAKENNTLTNDEKSFYKEATRMSKLIDNNVNLMSVINPYKALNVIANNKNNQPLNVVQSKPNSGFVRYMITLNPYNRILKTEIKLVDKLSTSGDNAGYHYITQQDLLNPQKNPDFNQNLITLNNFLNNYKDHEYEQLREKILIKNDKKVNLYSSTYSLSSLGAFISDILAKYYQAPFYNKQTHTFEFKQTQAILLAPHAIRSLVSAKPIKLENLNQILPFNDTLVSGEISVKNLKEFLINKNKSQNYNEPYQFSNTLKIEYENLQETKTKKKSRKKQSEQSYSDNEKKDGLILSHQKRIIKVFLKNSDGTYTELNDNQKIIISVMNSILKFNKKFSEALLNKTYADQTNIRDLLRTYLPKETQIDQKYDQSVFSKNDDRFYEKNETDQNKSLIYNHINESINNKQADHLRIGHWNVLHQTGLNDPKNYALAKTILFNKYDIIGLTEIWPAENNNQEQNNLPLIGIIKYLNKLSGSDDYDFLISDNLKGSENDKLATNEHTSTERIGIIYNKKKVKPIPFANNKIGYIYSNPLQPGKYEKAETDYSRPPFAIKFASIGQIKNDFTLVFGHLDSPGTYPSNTEYQKKEHLLKRTELVNKKVISTNQMINKYEQGSREVDDAERLVKVMEEIKKIDNNKDDDYFFLGDTNIRLHNQQWVFRTLIQNGYSSVFSDNDLFKSSLSVNPGEFSNPYDKIFSQVNGLTISNPSIYNLWNVYEDQIVDQEWLKKVRSLYPNEYHDKLFYIRNDISDHAPTFFDLGLNKNDTK" }, "paralog": false, "identity": 0.9954389965792474, "score": 1727.0, "evalue": 0.0, "description": "Internal stop codon at 107282, 109346. Nonsense mutation around 107282, 109346.", "psc": { "uniref90_id": "UniRef90_Q9PR90", "product": "Membrane nuclease A-hypothetical", "uniref50_id": "UniRef50_B5ZAN4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PR90", "UniRef:UniRef50_B5ZAN4" ] }, "pscc": { "uniref50_id": "UniRef50_B5ZAN4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B5ZAN4" ], "product": "Endonuclease/exonuclease/phosphatase family protein" } }, "id": "NKIECMJPJD_203", "locus": "GCA000828735_00167" }, { "type": "cds", "sequence": "contig_1", "start": 109344, "stop": 109643, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGTTTGTAAAAAAAATAAATTGAAAATGTTTGGTTTTATTGCTATTGGAACTGTTTTAACTAGTGCAATAATTCCTTTTTTAGTTACATGCGCAAAAGAGAAAAAAATAAAGCAAGAAGTTATAAAACCAAAACAAATAAGTAATTTAAATCTTGAAGCTAAAAATATTGATATTGTTTTAAAAAATAATGTCTTCAATAAAGAAACAGCAAAATTTACATTGGATTTTGACATAAATAAAGAGTTAAATTTACAAATGGTTGCTGTTTTTGATGTTTTTGATAAAAAAAATAACTGA", "aa": "MVCKKNKLKMFGFIAIGTVLTSAIIPFLVTCAKEKKIKQEVIKPKQISNLNLEAKNIDIVLKNNVFNKETAKFTLDFDINKELNLQMVAVFDVFDKKNN", "aa_hexdigest": "afa5ef12910c9643e0b230d4714468cf", "start_type": "GTG", "rbs_motif": "AGGAG/GGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11291.379699999987, "isoelectric_point": 9.548879814147949 }, "id": "NKIECMJPJD_204", "locus": "GCA000828735_00168" }, { "type": "cds", "sequence": "contig_1", "start": 109908, "stop": 111053, "strand": "-", "frame": 1, "gene": "atpD", "product": "MSC_0618 family F1-like ATPase beta subunit", "db_xrefs": [ "COG:C", "COG:COG0055", "SO:0001217", "UniRef:UniRef50_A0A449A4P1", "UniRef:UniRef90_Q9PR91" ], "nt": "ATGGTCCCAGTTGGTAATGATTCAAAAAATAATATTTATAATTTTTGGGGACATCCTTTATTAGAAACTAATAAAACACCTAAATATGTTGAAATGAATTCAACAATTTTAAACGAGCGATATGTTGATAAAAGTATAGAAATTGTAGAGACAGGAATTAAAGCAATTGATTTTTTTATGCCAATTCTAAAAGGATATAAATTAGGAATCTTTGGTGGTGCTGGAGTTGGTAAAACAGTTTTAATGAAAGAAATCATTTTTAACTTAAACAGACATAAACAAGCAAATTCAAATATTTTTATTGGTTCAGGAGAGCGTTCACGTGAAGCAATTGAATTATATAATGAATTGACTGCTTCTAATTTAATGCCTAATTCAGTAATGTTTATTTCTAAGATGAATGAAGCTCCTGGTGCACGTACTTCAATTGTACCAATTGGAATTACTGCTGCTGAATATTTACGTGATCAAAACAAAGAAAATGTTTTATTGTTTATTGATAATATTTATCGTTTTATTCAAGCTGAAAATGAAGTTTCTACTGCATTAGGTAAAAAACCGTCTGTTGGAGGTTATCAATCAACACTAGAAAGTGATGTTACACATATTCAAAATCGATTATTTAGAAATAAAAATGGATCAATTACCTCATTTCAAACAATTTTTTTGCCAATGGATGATTTAAGTGATCCATCAGCTGTCGCTGTATTTAATCATTTAGATGGAAAATTAGTATTATCGCGAGCACAAGCTGCTAAAAATATTTTTCCAGCATTTGATCCATTAGCAAGCTCAACAAACGCTATTGATCCTAAAATTATTGGTGAACGTCATTATAATGCAATTATCGAAACTAAAAAAGTTTTAAAAGCTTATAAAGATCTAGAAGATGTTATTTTAATTTTAGGTTTTGATGAACTAGATGCTGAAAGTAAAATTATTGTTAAAAAAGCTTTACAGCTAGAAATGTTCTTTACACAAAACTTTTTTATGACTGAACATTTCACAAAAGCTCCTGGTCAGTTTGTTCCATTAAAAGAGACTGTTGAAAGTGTAATTCGTATTTTAGAAGGTAAATATTTAAAACAAAGTCCAGATGTTTTTGCCTATATTGGTTCAAATAAGGATATTTCTCAAGATCATTAA", "aa": "MVPVGNDSKNNIYNFWGHPLLETNKTPKYVEMNSTILNERYVDKSIEIVETGIKAIDFFMPILKGYKLGIFGGAGVGKTVLMKEIIFNLNRHKQANSNIFIGSGERSREAIELYNELTASNLMPNSVMFISKMNEAPGARTSIVPIGITAAEYLRDQNKENVLLFIDNIYRFIQAENEVSTALGKKPSVGGYQSTLESDVTHIQNRLFRNKNGSITSFQTIFLPMDDLSDPSAVAVFNHLDGKLVLSRAQAAKNIFPAFDPLASSTNAIDPKIIGERHYNAIIETKKVLKAYKDLEDVILILGFDELDAESKIIVKKALQLEMFFTQNFFMTEHFTKAPGQFVPLKETVESVIRILEGKYLKQSPDVFAYIGSNKDISQDH", "aa_hexdigest": "4e2dcef1db19227d852b51c705f7db04", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_Q9PR91", "query_cov": 1.0, "subject_cov": 0.8392070484581498, "identity": 0.95, "score": 717.0, "evalue": 2.25e-259, "valid": true, "gene": "AtpD", "product": "MSC_0618 family F1-like ATPase beta subunit", "uniref50_id": "UniRef50_A0A449A4P1", "cog_id": "COG0055", "cog_category": "C" }, "pscc": { "uniref50_id": "UniRef50_A0A449A4P1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449A4P1" ], "product": "F0F1 ATP synthase subunit beta" }, "genes": [ "atpD" ], "id": "NKIECMJPJD_205", "locus": "GCA000828735_00169" }, { "type": "cds", "sequence": "contig_1", "start": 111353, "stop": 111502, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGTTAGATTATGCGATTAATAATTATGAAGATGCTCAAAAAGCATTCAATACTATTTCAACAACTCAAAATTATGATGATAAAATTATGAAAAACTACTTTGCTTTCATCCTAAAACAATACTCTGATTACTTAAATTTAAATTGA", "aa": "MLLDYAINNYEDAQKAFNTISTTQNYDDKIMKNYFAFILKQYSDYLNLN", "aa_hexdigest": "ea148a5c6c834445f126bb93b84b1267", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5881.510200000001, "isoelectric_point": 4.460405921936036 }, "id": "NKIECMJPJD_206", "locus": "GCA000828735_00170" }, { "type": "cds", "sequence": "contig_1", "start": 111542, "stop": 112441, "strand": "-", "frame": 2, "gene": "atpA", "product": "MSC_0619 family F1-like ATPase alpha subunit", "db_xrefs": [ "COG:C", "COG:COG0056", "SO:0001217", "UniRef:UniRef50_A0A449A4K5", "UniRef:UniRef90_Q9PR92" ], "nt": "ATGACAGTGAAAACTTTAAATGAGCAATTAAATACTGGAATTGTAGCAATTGATTTATTAATTCCGATTGGCAAAGGACAAAGGGAATTAATTATTGGTGATCGTCAAACTGGAAAAACGCATATTGCTTTAAATACTATTATTAATCAAAGCCGTGCTGGCACAAAATGTATTTATGTAGCAATTGGTCAAAAACGCGAATCATTAACTACTGTTTATGAAACTTTAAAGGCTCATGACGCATTAAAAAACACAATTATTATCGATGCTCCTTTAACAGCATATGAACAATATTTAGCACCATATATTGGAATGGCTCATGCTGAAAACTTATCGTATCAAGATGATGTATTAATTGTATTTGATGATCTAACAAAACACGCTAATATTATTCGTGAAATGGCTTTATTAACAAATAAACCAGTTGGTAAAGAAGCAATGCCTTCAGATGTATTCTTCTCACATTCATCATTACTTGAACGTGCTGGTTCATTCAAAAATCGTAAAACAATTACTGCTTTGCCGATTTTACAAACAGTTGATGGGGATATTACTTCATTAATCTCTTCAAATATTATTTCTATTACTGATGGTCAAATTGTAACTTCAACTGATTTATTTGCTGCTGGTAAAGTTCCTGCTATTAATATTGATTTATCAGTTTCAAGAACAGGTTCAAGTGTACAATCAAGAATGATTACTAAAGTAGCAGGTGAAATTAATAAAATTTTTAGACAATACAAACGTCATTTAAAATTAGCAATGTTAGATTATGAATTTAATAAAGAAACTAGCCTTTTATTGCATAAAGGTAAATTAATAGATAAAATGTTTTTACAAAAAGGTTTCTCTTTATTTTCATACCGTTTTATTGTTTTAAGTACTAAGCTAATTGCCTGA", "aa": "MTVKTLNEQLNTGIVAIDLLIPIGKGQRELIIGDRQTGKTHIALNTIINQSRAGTKCIYVAIGQKRESLTTVYETLKAHDALKNTIIIDAPLTAYEQYLAPYIGMAHAENLSYQDDVLIVFDDLTKHANIIREMALLTNKPVGKEAMPSDVFFSHSSLLERAGSFKNRKTITALPILQTVDGDITSLISSNIISITDGQIVTSTDLFAAGKVPAINIDLSVSRTGSSVQSRMITKVAGEINKIFRQYKRHLKLAMLDYEFNKETSLLLHKGKLIDKMFLQKGFSLFSYRFIVLSTKLIA", "aa_hexdigest": "0d018a92c2fdd0be822aa5dc72f499e2", "start_type": "ATG", "rbs_motif": null, "genes": [ "atpA" ], "truncated": "both", "pseudogene": { "start": 111272, "stop": 112741, "observations": { "mutation": [ 111355, 111544, 112519 ], "stop": [ 111355, 111544, 112519 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PR92", "query_cov": 1.0, "subject_cov": 0.5851272015655578, "identity": 0.993, "score": 565.0, "evalue": 8.54e-200, "gene_start": 1, "gene_end": 299, "reference_start": 123, "reference_end": 421, "reference_sequence": "MQNTKANPRIKSIFNYIVTVSGIYDYQHHQIFVLKNDPTVKLFVISAKEDVAYLLISNEHANINIGDEIIETNREENVVTDLNYFGKIIDINNNFIWPKPLNNLPSAKIAQTNPAFGLAHNLMTVKTLNEQLNTGIVAIDLLIPIGKGQRELIIGDRQTGKTHIALNTIINQSRAGTKCIYVAIGQKRESLTTVYETLKAHDALKNTIIIDAPLTAYEQYLAPYIGMAHAENLSYQHDVLIVFDDLTKHANIIREMALLTNKPVGKEAMPSDVFFSHSSLLERAGSFKNRKTITALPILQTVDGDITSLISSNIISITDGQIVTSTDLFAAGKVPAINIDLSVSRTGSSVQSRMITKIAGEINKIFRQYKRHLKLAMLDYEFNKETSLLLHKGKLIDKMFLQKGFSLFSYRFIVLSTKLIAWGILKGVKDEQKAFMLLDYAINNYEDAQKAFNTISTTQNYDDKIMKNYFAFILKQYSDYLNLNWEVELEHSFIPLQNEFLMNVAKFLGDK" }, "paralog": false, "identity": 0.9877300613496932, "score": 940.0, "evalue": 0.0, "description": "Internal stop codon at 111355, 111544, 112519. Nonsense mutation around 111355, 111544, 112519.", "psc": { "uniref90_id": "UniRef90_Q9PR92", "gene": "AtpA", "product": "MSC_0619 family F1-like ATPase alpha subunit", "uniref50_id": "UniRef50_A0A449A4K5", "cog_id": "COG0056", "cog_category": "C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PR92", "UniRef:UniRef50_A0A449A4K5", "COG:COG0056", "COG:C" ] }, "pscc": { "uniref50_id": "UniRef50_A0A449A4K5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449A4K5" ], "product": "ATP synthase F0F1 subunit alpha" } }, "id": "NKIECMJPJD_207", "locus": "GCA000828735_00171" }, { "type": "cds", "sequence": "contig_1", "start": 112517, "stop": 112807, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAAACACTAAGGCAAACCCTCGTATTAAATCGATTTTTAACTATATTGTAACTGTTAGTGGAATCTATGATTATCAACATCATCAAATATTTGTTTTAAAAAATGATCCAACAGTAAAATTATTTGTTATTTCAGCAAAAGAAGATCTTGCTTATTTATTAATTAGCAATGAACATGCTAATATTAATATCGGCGATGAAATCATTGAAACAAATCGTGAAGAAAATGTTGTTACTGATTTAAATTATTTCGGGAAGATTATAGATATTAATAATAATTTTATTTGA", "aa": "MQNTKANPRIKSIFNYIVTVSGIYDYQHHQIFVLKNDPTVKLFVISAKEDLAYLLISNEHANINIGDEIIETNREENVVTDLNYFGKIIDINNNFI", "aa_hexdigest": "f363e1cf2717b0b7fc56c0eb68d3a22b", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11100.450399999987, "isoelectric_point": 5.1671987533569315 }, "id": "NKIECMJPJD_208", "locus": "GCA000828735_00172" }, { "type": "cds", "sequence": "contig_1", "start": 112823, "stop": 113122, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCTATATTAACAAATCAAACCGATATTAAAACAAATAAGGACAAAGCTAAGGATTTTAAAAATGCATATGATAGTGCTTTAAAATCTCTTGAAAGACAAAAACAAGTAAATAAACAAATTCGTCGTAAGATTGGAATTGCGTTTATAGTAATTAGCTTACTGGTTTTAATAATTAATTTAATTATTTATGGATTGATTAAAAAACTAAAAAATAAAAAAGTTATTCTAATTATTAATAGTGTGATAATGGTGTTGACAATCATTGTTTTAATAATGGGAATTATTCTAATAATTTAG", "aa": "MSILTNQTDIKTNKDKAKDFKNAYDSALKSLERQKQVNKQIRRKIGIAFIVISLLVLIINLIIYGLIKKLKNKKVILIINSVIMVLTIIVLIMGIILII", "aa_hexdigest": "178e6260af7948fd57d2ce8d66668bd6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11258.823399999987, "isoelectric_point": 10.334491539001462 }, "id": "NKIECMJPJD_209", "locus": "GCA000828735_00173" }, { "type": "cds", "sequence": "contig_1", "start": 113375, "stop": 113842, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTAATATTAAAGTTAAAATTATTGATCAAGTTAATCCTAAACTACAACGTACATATATTATTAATAAATATGATCCAATTTTAGATATTAATTTTAATAGTCTAAAATTAAATGAAGTTAATGCTATTAAACAAATTTTTGTTAACTTGTATAAACATTTAGGACTAGATGAGAAAATTGATTATAAGAAATTACGTAACTTTTATATTAGAAATGCTTTATTTACAATGATTGAAGCTGCTCAAAAACTTATTTTGCGATTCAATCAAATTGATAAAGATAATAAAATTATTGGTCAAAAAACTTTTGCAACATTACAAAATGAATATTTAGAAAAATACAAACAAAAACTTGTAAACAACACCGATGATAAGGAAAGATTATTAAATGAGTTTAATAATTTAACAAAAGAAAATTTCTTTCGTTATTTAAATAACACTTTAATCAATAATGATTATTATTGA", "aa": "MINIKVKIIDQVNPKLQRTYIINKYDPILDINFNSLKLNEVNAIKQIFVNLYKHLGLDEKIDYKKLRNFYIRNALFTMIEAAQKLILRFNQIDKDNKIIGQKTFATLQNEYLEKYKQKLVNNTDDKERLLNEFNNLTKENFFRYLNNTLINNDYY", "aa_hexdigest": "d60bf1994ef0ad7afbc0c1cb7f8704c1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18720.423400000007, "isoelectric_point": 9.415365791320802 }, "id": "NKIECMJPJD_210", "locus": "GCA000828735_00174" }, { "type": "cds", "sequence": "contig_1", "start": 114227, "stop": 114988, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGAAATAAATTGAAAATAAAATTAAAATTTTTAGCTTCACTAGCAACAATACCAATTGTAGCTTCACCATTAATTTTTTCTGTTGCAGCTAATGAGGATGGTAAACAAGAAAACAATAATGGTAATAATCATGATCCTAATCAACAAAAAAAACCAAAAATTCCTAAAAACGATCCTAATTTTAATACTTTTAAAACTGATGCTGATAAAACTGTTAAAGATAGTTTAGAAAAAGGAATTAATGCTGCTATTGTTTATGTAAAATCACGTCAAGAAGAAATTTTAGAAAATAAAGAAATTGAGTTTAAGAAAAAAATTCAACAACTAATTTATTTAAAAAATCTGCAGTCATATCTTGAGAAAAATAAAGAAAACATCTTAAAAAATCCTAATGATTATGGTTTTTATTTAAATACTCCTCAAATTTTAGGAACTCTTAAAAATTATGATATTAAAGATATTGAGTTTAATGGAGAAACATATAAGCAAATCAAAGTTGGCAAAACTGATCCTTTAAATTATCAAAAAGCCGTTGCTCCAAAAGGAAAAATTAGTGATGTACAAAGTGATCAAATTAATGATGTTGAAGAAACAAAATACAAAGATACGTTAAAAAAATATGAATCTGAATTTTTAAAAGAAATTAATAAATTAATTTATGATGAAAATGATGTTCCACAAATTAACAAAGATGTTGAGTTAACACGTGATGAAAAAGGACAATTTAATACAACTTTACCTAAAGGTTATAATGATTGA", "aa": "MRNKLKIKLKFLASLATIPIVASPLIFSVAANEDGKQENNNGNNHDPNQQKKPKIPKNDPNFNTFKTDADKTVKDSLEKGINAAIVYVKSRQEEILENKEIEFKKKIQQLIYLKNLQSYLEKNKENILKNPNDYGFYLNTPQILGTLKNYDIKDIEFNGETYKQIKVGKTDPLNYQKAVAPKGKISDVQSDQINDVEETKYKDTLKKYESEFLKEINKLIYDENDVPQINKDVELTRDEKGQFNTTLPKGYND", "aa_hexdigest": "663c5652e33dc006c431a0e161a4c637", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 29181.673200000023, "isoelectric_point": 8.616795921325686 }, "id": "NKIECMJPJD_211", "locus": "GCA000828735_00175" }, { "type": "cds", "sequence": "contig_1", "start": 114978, "stop": 115151, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTAGATAAATATAAAAATATTACTAATAATATTTTAGAACTTGAAGCTAAACAACAATTAGGATTAACTCTAAGTGAATTTTTAGATCTTGATAATCTAAAACAAGAACAATATATAACTAATATGCAAATTAGATTAAATTTAGTGGAGTATAAAAAAGATGAGAAATAA", "aa": "MVDKYKNITNNILELEAKQQLGLTLSEFLDLDNLKQEQYITNMQIRLNLVEYKKDEK", "aa_hexdigest": "67df1e428446086b8e2527d75d5ee038", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6831.774300000001, "isoelectric_point": 4.927224540710449 }, "id": "NKIECMJPJD_212", "locus": "GCA000828735_00176" }, { "type": "cds", "sequence": "contig_1", "start": 115356, "stop": 115478, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATAACGATGGTGGTGTTTATGAATCATAATTATAATATTCAAATTACATTACTTGAAAACAAAAAGTTTAATATTGATAATGGTTTATTATATGTAAATGTTAATGAAGAAAATACATGA", "aa": "MITMVVFMNHNYNIQITLLENKKFNIDNGLLYVNVNEENT", "aa_hexdigest": "2ab2167f3ccc4ac95e20c6c6783d1c30", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4745.411000000002, "isoelectric_point": 4.830996131896971 }, "id": "NKIECMJPJD_213", "locus": "GCA000828735_00177" }, { "type": "cds", "sequence": "contig_1", "start": 115447, "stop": 116361, "strand": "-", "frame": 3, "gene": null, "product": "MSC_0622 family F1-like ATPase gamma subunit", "db_xrefs": [ "RefSeq:WP_006688472.1", "SO:0001217", "UniParc:UPI0000139197", "UniRef:UniRef100_Q9PR95", "UniRef:UniRef50_Q9PR95", "UniRef:UniRef90_Q9PR95" ], "nt": "ATGCAATTAAAAGATTTAATTAATAAAAAAAAGAATTTAAATAATATTAATTTAAAAGTGAGTAACGAACGTAATATTTTTTTAATTAATATTATGAAACTAAATCAGCGATTAACTTTTTTTAGTAAAAATGCTTTTGAAATAAAAGAATCAATTCTAAGTTTAAAGCGTATTTATAATATTAAACATGATATGTTAAGACATGAGGAACGAAAGATTTTTAAGTTTTTAAATAAAATTAATGATCGTGTTTTATGGATTTATTTAACTGAAGAGCAAAAATATAGTACTGATTCATATTCGCGTTATGAACAAAAAATCTTAAAAACGATTAAATCAAATCGTGATGATTTTATTTTGATCGGTCAAGGAGCAATTGAATTTGGTAAGAATCATAATTTAAATGTTCTGCAAACATTTAATGACTCAAATATTAAAAATTTAACTACACAATTAACTAAAATGATTATGATTTTATATACTTTTGATAATTATAAAAAAGTTAATTTTGTTATTAATTCTAATAAAAACTATGATGGACACTTTACTATATTACCAATGAATGAATTTAGTTTTGATAAATTTATTAATTTACAAAAATGCGATTCTAATATTATTGATTTTCAAAAAGTAAAAATTTATCCAAATTTAAATGAATTTATTAATGTCCAAATTAATGTTTTCTTAGTTAATATTATTAATACTTTAATTACTGAATCATCTTTTTATAAAACAAAAAATGGACTAGTAGCTACTAATAATATCCTAAAAGAACTAGATGATAACCTTTCAAAAATTCAACGTAAAATTACACGTGTAAAGACAGAGTTGCAAATTGAAGAAATAAATTTATTAGCAAGACAAAATATGAATGAAGATGATAATGATAACGATGGTGGTGTTTATGAATCATAA", "aa": "MQLKDLINKKKNLNNINLKVSNERNIFLINIMKLNQRLTFFSKNAFEIKESILSLKRIYNIKHDMLRHEERKIFKFLNKINDRVLWIYLTEEQKYSTDSYSRYEQKILKTIKSNRDDFILIGQGAIEFGKNHNLNVLQTFNDSNIKNLTTQLTKMIMILYTFDNYKKVNFVINSNKNYDGHFTILPMNEFSFDKFINLQKCDSNIIDFQKVKIYPNLNEFINVQINVFLVNIINTLITESSFYKTKNGLVATNNILKELDDNLSKIQRKITRVKTELQIEEINLLARQNMNEDDNDNDGGVYES", "aa_hexdigest": "c8d5dda3918a4bfc15fd5022402939ad", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0000139197", "ncbi_nrp_id": "WP_006688472.1", "uniref100_id": "UniRef100_Q9PR95", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139197", "RefSeq:WP_006688472.1", "UniRef:UniRef100_Q9PR95" ] }, "ips": { "uniref100_id": "UniRef100_Q9PR95", "uniref90_id": "UniRef90_Q9PR95" }, "psc": { "uniref90_id": "UniRef90_Q9PR95", "product": "MSC_0622 family F1-like ATPase gamma subunit", "uniref50_id": "UniRef50_Q9PR95" }, "pscc": { "uniref50_id": "UniRef50_Q9PR95", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR95" ], "product": "Uncharacterized protein UU050" }, "genes": [], "id": "NKIECMJPJD_214", "locus": "GCA000828735_00178" }, { "type": "cds", "sequence": "contig_1", "start": 116364, "stop": 116612, "strand": "-", "frame": 1, "gene": null, "product": "putative protein UU049", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR96", "UniRef:UniRef90_Q9PR96" ], "nt": "ATGATTCCAATATTAGTAACTAAAGCTAATTCTACAACTGATGCAATTAATTTTAGAAATGATCAAAATCCTGTTTATAATAATTTTTTAATTTCATATAACCAAAAAATTAAAAAATTATTACTTCAAAATCATCCTGTTCAAATTCTTCCTCATTTGATTTTATTTAAATCAAATCTTAATGGTAGTTTAGTGCTCGTATTTAGTGAAAAAATTATCGCTTCAATTGAACAAAAATCAGGAAATTAA", "aa": "MIPILVTKANSTTDAINFRNDQNPVYNNFLISYNQKIKKLLLQNHPVQILPHLILFKSNLNGSLVLVFSEKIIASIEQKSGN", "aa_hexdigest": "29ced18b53ff2d06fb560b1a4884a385", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 116364, "stop": 116879, "observations": { "mutation": [ 116669 ], "stop": [ 116669 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PR96", "query_cov": 1.0, "subject_cov": 0.47953216374269003, "identity": 1.0, "score": 157.0, "evalue": 1.02e-47, "gene_start": 1, "gene_end": 82, "reference_start": 90, "reference_end": 171, "reference_sequence": "MKKNKSNLTPTTNYFDVFNTYKEKKASVDLITYEELMASVLFDNKLGFESEVYLDFVKKFTLAFEKKLDIWFENFIINFNLNLKFSTTIMIPILVTKANSTTDAINFRNDQNPVYNNFLISYNQKIKKLLLQNHPVQILPHLILFKSNLNGSLVLVFSEKIIASIEQKSGN" }, "paralog": false, "identity": 0.9941520467836257, "score": 333.0, "evalue": 1.32e-119, "description": "Internal stop codon at 116669. Nonsense mutation around 116669.", "psc": { "uniref90_id": "UniRef90_Q9PR96", "product": "Uncharacterized protein UU049", "uniref50_id": "UniRef50_Q9PR96", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PR96", "UniRef:UniRef50_Q9PR96" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PR96", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR96" ], "product": "Uncharacterized protein UU049" } }, "id": "NKIECMJPJD_215", "locus": "GCA000828735_00179" }, { "type": "cds", "sequence": "contig_1", "start": 116667, "stop": 116879, "strand": "-", "frame": 1, "gene": null, "product": "putative protein UU049", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR96", "UniRef:UniRef90_Q9PR96" ], "nt": "ATGAAAAAAAATAAATCTAATCTGACACCTACAACAAATTATTTTGATGTATTTAATACTTATAAAGAGAAAAAAGCAAGTGTTGATTTAATTACATATGAAGAATTAATGGCATCAGTTTTATTTGATAATAAATTAGGTTTTGAATCAGAGGTTTATTTAGATTTTGTTAAAAAATTTACATTAGCTTTTGAAAAAAAACTAGATATTTGA", "aa": "MKKNKSNLTPTTNYFDVFNTYKEKKASVDLITYEELMASVLFDNKLGFESEVYLDFVKKFTLAFEKKLDI", "aa_hexdigest": "09f2186cc324674883ee130d6d4f00a0", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 116364, "stop": 116879, "observations": { "mutation": [ 116669 ], "stop": [ 116669 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PR96", "query_cov": 1.0, "subject_cov": 0.4093567251461988, "identity": 1.0, "score": 138.0, "evalue": 3.46e-40, "gene_start": 1, "gene_end": 70, "reference_start": 1, "reference_end": 70, "reference_sequence": "MKKNKSNLTPTTNYFDVFNTYKEKKASVDLITYEELMASVLFDNKLGFESEVYLDFVKKFTLAFEKKLDIWFENFIINFNLNLKFSTTIMIPILVTKANSTTDAINFRNDQNPVYNNFLISYNQKIKKLLLQNHPVQILPHLILFKSNLNGSLVLVFSEKIIASIEQKSGN" }, "paralog": false, "identity": 0.9941520467836257, "score": 333.0, "evalue": 8.38e-120, "description": "Internal stop codon at 116669. Nonsense mutation around 116669.", "psc": { "uniref90_id": "UniRef90_Q9PR96", "product": "Uncharacterized protein UU049", "uniref50_id": "UniRef50_Q9PR96", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PR96", "UniRef:UniRef50_Q9PR96" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PR96", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR96" ], "product": "Uncharacterized protein UU049" } }, "id": "NKIECMJPJD_216", "locus": "GCA000828735_00180" }, { "type": "cds", "sequence": "contig_1", "start": 116866, "stop": 117345, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTATTCACTTTAAAAAAACGCTTAAGCGTAAAGAGTAGTTTAAATACTTTATTAAATTTAGTAGTCACTCAAACAATTCTAAGTTTAAGCTTGTTTTTAGTAACATTATTTAATTCTAAGAGTGTGGTTTCTTTAATTAATGTTTTTATTACAATTACTGTACAAATGAGTGTGTTTGGAATCTACATATTTCAAAATAAAAACATTTCAACTAAATTATTGGTGCTATTAAAGGTTATTATGATTTTAATTATTTTAACCGCAGCAATTGTAGGTTTTGATTATTTATTAACATCTGATCATCATAATAACTATTTATTCTCAAACATTCAACCGAAAATGAATTTAGTACAAATCATGTTGTTATTAAATTTTAGTTTGAGTTTTACTTTAATTAGTTATTTAACAATTAAATTTGCAATGGTAATTTTTAAAATCAATAAGCTAAATAAGGAGTTAAACAATGAAAAAAAATAA", "aa": "MVFTLKKRLSVKSSLNTLLNLVVTQTILSLSLFLVTLFNSKSVVSLINVFITITVQMSVFGIYIFQNKNISTKLLVLLKVIMILIILTAAIVGFDYLLTSDHHNNYLFSNIQPKMNLVQIMLLLNFSLSFTLISYLTIKFAMVIFKINKLNKELNNEKK", "aa_hexdigest": "e2898c6010c385986f08a696b8a8c383", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18151.821600000007, "isoelectric_point": 10.095829582214353 }, "id": "NKIECMJPJD_217", "locus": "GCA000828735_00181" }, { "type": "cds", "sequence": "contig_1", "start": 117973, "stop": 118356, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATGAATACAAAAACATTTACTTCCGTTAATAGAGTAATTTATGATGATAACTATTCGCTAAAGCAACAACAAAAAAGTAGCTTTATCAATCAATTTTTAAAAGGATTGTTATCTGCTATTACTTTATTATTCTTTATTTTACTTTTAATCTTTGCTGAAAATACGTTGTTTGGTTTAGGTTTTGGTGATGAGAATAAATCAATGATGATTTCAAAATCGCTAAATGCTTTTTTTGATTTACACAGCCCTAAATATTTACAACTTAACTTTTTAATTGTATTTCGCTTTTTTATTTTAAGTTTTACACTGTTTTATGCCTTAATCAAAAATTTCACAAATCTTTATTGGCATCGAGCAACCATTAAAAAATACTTACCATGA", "aa": "MMNTKTFTSVNRVIYDDNYSLKQQQKSSFINQFLKGLLSAITLLFFILLLIFAENTLFGLGFGDENKSMMISKSLNAFFDLHSPKYLQLNFLIVFRFFILSFTLFYALIKNFTNLYWHRATIKKYLP", "aa_hexdigest": "d1b638dde52905d3f07f0d34ea1cc2a3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14997.519199999979, "isoelectric_point": 9.776324272155762 }, "id": "NKIECMJPJD_218", "locus": "GCA000828735_00182" }, { "type": "cds", "sequence": "contig_1", "start": 118386, "stop": 118892, "strand": "-", "frame": 1, "gene": null, "product": "MIP family Ig-specific serine endopeptidase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021DFAD80" ], "nt": "TTGTTTCCTCTTACACAAATAAATCGTGGTGATTTCTTGTCATATAATATTGGTTATCGTAGTTTTACTAATAAACCTGGATTGCTTGATGCTTTTATTGCTAGTCCTATTACTGGTAAAACTGTACATAAATCAACTGATAATCAAAATTATATTGGGTTTGGTTTAAATTATTTACCGATGCATTATTCTCCAATTGGGGGTTCATCAGGGACAAGTCTACGTAATCAAAAAAATGAATTAGTGGGAATTTGGCATGTTGGTAATGGTTTTGCCCAAACAGGTTTGGCTGCTGCTTTTCGATCTGAAGGCTATGATTATCATGGATTATATGGTAGTTATAACTTACCACAATATGATTTGATTTATGGTGGTGGAAAAGATCAAAAAACTTCCTATCGCCAAGCAATGATGGCGTTATATAAAAATAAAAATATTGCAACCGCTTTATTCCCTAATGGTTTTGGTGAAGATCAAATCCCCAGTGCTTTTAAATTTAAAAATTAA", "aa": "MFPLTQINRGDFLSYNIGYRSFTNKPGLLDAFIASPITGKTVHKSTDNQNYIGFGLNYLPMHYSPIGGSSGTSLRNQKNELVGIWHVGNGFAQTGLAAAFRSEGYDYHGLYGSYNLPQYDLIYGGGKDQKTSYRQAMMALYKNKNIATALFPNGFGEDQIPSAFKFKN", "aa_hexdigest": "efbe96d508f148e4ee720b29783b1ae2", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002AFE92B4", "query_cov": 0.9107142857142857, "subject_cov": 0.9444444444444444, "identity": 0.601, "score": 200.0, "evalue": 4.41e-63, "valid": false, "product": "MIP family Ig-specific serine endopeptidase", "uniref50_id": "UniRef50_UPI0021DFAD80" }, "pscc": { "uniref50_id": "UniRef50_UPI0021DFAD80", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021DFAD80" ], "product": "MIP family Ig-specific serine endopeptidase" }, "genes": [], "id": "NKIECMJPJD_219", "locus": "GCA000828735_00183" }, { "type": "cds", "sequence": "contig_1", "start": 118941, "stop": 119090, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGATTCAAAATACAATCAGGCTTTTGACACAAATAAATATGATGAATTATATGCTGTAGGTTGGCCAAGCTCTGCTTCTGATTATTTCTTAGACCCAATTAAAGATCAGAAACAATTTGAATCAAGACGTGAAAGTTATAGTTTATGA", "aa": "MDSKYNQAFDTNKYDELYAVGWPSSASDYFLDPIKDQKQFESRRESYSL", "aa_hexdigest": "12c003be40d8d66d56290ef5647cdbc6", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5835.2501999999995, "isoelectric_point": 4.518097496032715 }, "id": "NKIECMJPJD_220", "locus": "GCA000828735_00184" }, { "type": "cds", "sequence": "contig_1", "start": 119229, "stop": 119648, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01732.22" ], "nt": "ATGGATTATCAAAAAAGAAATGATAATAAATATCCTACTAAATGGTATTTTGGCACAAATTTACATGTTGCAGAAGCATTAAAGTCAACAACAACAGTTTTTGGTATTAATAAGATAATGCCAACAGTTAAGACTAAAACTACTTTGGGTTTAGCGAACGCTGATGATAATATTTATCGTTTTAGTTTAGTTAGTAAAGATAATCAACCAAATATGGATAAACCTATTAGTAATGGTATTAAAACAATCTATGATGGTCGTGACTTCTTAAAATTAAATCCTAGTGACTTATTAACATCAAAATTAAAAAATAAATACCATAACCTACAAGAATTTGTTGATTTTGCTGTTTTTGAAATTGATTTTGAAAAAATAAAACTCGGCTCTATAACGAAAAATTTTTATAGTGGATCAGATTGA", "aa": "MDYQKRNDNKYPTKWYFGTNLHVAEALKSTTTVFGINKIMPTVKTKTTLGLANADDNIYRFSLVSKDNQPNMDKPISNGIKTIYDGRDFLKLNPSDLLTSKLKNKYHNLQEFVDFAVFEIDFEKIKLGSITKNFYSGSD", "aa_hexdigest": "2ce66634d9ac1421a719d33b07bbccf7", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01732.22", "name": "Mycop_pep_DUF31", "length": 145, "aa_cov": 0.9064748201438849, "hmm_cov": 0.9310344827586207, "evalue": 8.358406518015442e-31, "score": 103.39796447753906, "start": 1, "stop": 126 } ], "seq_stats": { "molecular_weight": 15959.943499999985, "isoelectric_point": 9.155622673034667 }, "id": "NKIECMJPJD_221", "locus": "GCA000828735_00185" }, { "type": "cds", "sequence": "contig_1", "start": 119652, "stop": 119771, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGCTAGAACTTTGCCTAATAATATGTATAAGCAAATTGCTTTACAAACATTTGGCATTCAAATTCAAACGCCAAATGGTAAACCACGCGAATCTGATGTAGTAGCTGGAACAATGTGA", "aa": "MARTLPNNMYKQIALQTFGIQIQTPNGKPRESDVVAGTM", "aa_hexdigest": "f0c7defd02a18a783b1589aecbea20b4", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4320.9684000000025, "isoelectric_point": 9.693869209289552 }, "id": "NKIECMJPJD_222", "locus": "GCA000828735_00186" }, { "type": "cds", "sequence": "contig_1", "start": 120003, "stop": 120989, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAATTAAAAAACTTATTGCAAGCGTTAGTATAATCACACCAATTGTGTTTGTTTCAGCTTTAGCAGCGTCTTGTGTTAATAATAAACATGAAGATAAAAATATTGTGCATAATAGTAGTGACCATGGTAATAATAAAAAAAATAATTTTACTAATGATTTAAATCAAAATTTAACAACCCACGAATTTAAATCAAATTTAATAAGTACTTCCCAATTAGATAATATAGCCAAATTAATTAATTTTACTTATGAAAATAAAGCTAATACTTATTTAAAAAATATACTTATTAATCAATTAAAACATTCACCTATTCAAAATCAAGATTTTAAACTAGAAATTATAGGTTTATATCCTAAACAAAATTCCTTGCAGGATTTAATTATTTATTATAAGTTAACTAACCAAAAAACAAAGGAGATTAAAGGTTATTATTTTGAATTAAATGGTTTTAAAAAACCAGATAACACCATTTTTTCAAATCAATTAACGCCTGAGCTAAAAAAAATTATTGATACTATTCAATTCAAAAAAACTTTTGATCTCGTAATTAATAATCAACAAGTATTAAATTATGAAAATGTTTTACCTTCACAAATTAAAAATCAATTATTAATGGGTTTAAAAGTTATTCAAAAAGAGTATACAGACAAAATTAAACTAAGTGTACTTGATGTTTTATTCATTAACGAGGGTGGGCAAATTAACGCTAATAAGTTGGGGGCTTTTTCTTTATTATTAGAGGTTTTAGATCTTAAAAGTAAAAAAACTTACCAAATTCTTATTCCTGTTGATAAATTTAAAACTAATCCTTATGGTGCTGATGAATATGATTTATTGCCAACGCAAGTTAATAACGGATTTGCACCAACTAGCTTAGCTCAAATTAATCAATACAATAATGCTGATCAACAAACACGTTATTTATATGATAATGAAAATTATTTAAAATCATTAAAAGCATATCAAAGAAATGTAAATTGA", "aa": "MKIKKLIASVSIITPIVFVSALAASCVNNKHEDKNIVHNSSDHGNNKKNNFTNDLNQNLTTHEFKSNLISTSQLDNIAKLINFTYENKANTYLKNILINQLKHSPIQNQDFKLEIIGLYPKQNSLQDLIIYYKLTNQKTKEIKGYYFELNGFKKPDNTIFSNQLTPELKKIIDTIQFKKTFDLVINNQQVLNYENVLPSQIKNQLLMGLKVIQKEYTDKIKLSVLDVLFINEGGQINANKLGAFSLLLEVLDLKSKKTYQILIPVDKFKTNPYGADEYDLLPTQVNNGFAPTSLAQINQYNNADQQTRYLYDNENYLKSLKAYQRNVN", "aa_hexdigest": "7829d2447d660140864a35ccca6d94ba", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 37694.6596, "isoelectric_point": 9.200363731384279 }, "id": "NKIECMJPJD_223", "locus": "GCA000828735_00187" }, { "type": "cds", "sequence": "contig_1", "start": 121004, "stop": 121462, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGGAAATAGTTATCCCCAAGGTTTAGACTTTAGTCGTGTCCCTCAAATTCGCTCATTACGGGGTTTAATATTTAAAGACGAACAAAAAGCTTCAAACAATCGTGAACGTAAATTAAGAAGAGTTAATTTCTTTAATGATAAAGAAAATTATGAAATGTCAATTAATGATTTAAATGAAGCTGGTTTTAGTGAACATATTGTGACTAATGAACCAATGCCTCCTAAAAGTAAAATTACTTTTAGTAATGGTAATGCTACAAAACGTATTTACATTAAAGGCAATGGTAGTCTAACTGCAAGTGGTATTCAAAATTTAGCTACATTATTTAATCTAGCAGAAAGTTTAGATTCTAAATCTGTTGTTGTTGATTCTAATAATTCAGAATTAAAGTCACAATTAGAAGGTTTAGGATATAAAGTAAGTGATGCTTCTGACGCTAACTATATTGATATTTAA", "aa": "MGNSYPQGLDFSRVPQIRSLRGLIFKDEQKASNNRERKLRRVNFFNDKENYEMSINDLNEAGFSEHIVTNEPMPPKSKITFSNGNATKRIYIKGNGSLTASGIQNLATLFNLAESLDSKSVVVDSNNSELKSQLEGLGYKVSDASDANYIDI", "aa_hexdigest": "ddbc3f4adc5ee1667260f88898d309fe", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16955.687799999985, "isoelectric_point": 6.598859214782716 }, "id": "NKIECMJPJD_224", "locus": "GCA000828735_00188" }, { "type": "cds", "sequence": "contig_1", "start": 121736, "stop": 121921, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGGAAATCAGATTCAGGACAAAAGTTCTCTGATATTCTAAAAGCCTTACCAAATGAATTGCCATTGCTTGAATTGTTCTTTTCTGCTGGTTCACATAATACGTCAGCACTTTCTGCTCTTGAAACTAAACATATTAAAGAACTTGGGTTGTATACATTAGGTAATTCATTATTAGATGAATGA", "aa": "MGKSDSGQKFSDILKALPNELPLLELFFSAGSHNTSALSALETKHIKELGLYTLGNSLLDE", "aa_hexdigest": "60e10f4dcb7edc0a8b275996915f0ac2", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6591.407799999998, "isoelectric_point": 5.171461677551267 }, "id": "NKIECMJPJD_225", "locus": "GCA000828735_00189" }, { "type": "cds", "sequence": "contig_1", "start": 122534, "stop": 123379, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATTTATTAAACGTAAAACAAAATTATTAACGATTACAATTGGTGCAGTTGCTGTGAGTTCAATTTTATTAGGAGGAATTTTTTATGGTACAAGTCAAAAAAGTCCTTCAAGTTTTGGTATTGCTTCTATTGATCAAAAAGAAAATTTTATTAATAAAGACAATTTAGATTACCAAAAAGCAAGACCATCAATTAAAGATAATAACTTAAAAGAAATTCCCAAACCAAAACCTCAGCCAAAACCAGAACCACAACCAACACCATTTCCAGATCCCATCCCAACACCTCCAAAAAAAGAAGAGTTAAAAAAACCAGAGATTAAACCAGAAGAGCCTAAAAAGCCTGAAATTAAACCAGAACCAATTCCTAAACCAAAACCTCAGCCTATTCCTCAACCAACACCTCCTGTTGAAACTAAACCAAAAGAAGAATTATTACCACCAAGCCCACCACCTCCAAAAGAAGAACCAAAACCAGAACCAGATCCACAACCACAACCACAACAAATTCCTAACCAAAGTACTGTTAGAAAAATTGAATTGAATGGTGTTTTAGTGGACGCTGAAGTTGAAGTTCCACCCCCTCGTCAAACTTTTAAATATGATCAGGATAATGGATTATCAAATCTTAATCCCTATACAAATATAAGTGTTGGGAAAATTAAAAAAGTTTTTGTAACAGATGAGTTGCGGCACAAATCAGCAGATTTAGTGCGCGGGAATTTAAAGCGTGGCGATTATCAAAGTTTAGTTAAAGATTTATTAGATCCAAATATAAAACCAGAGGAAATTGATAGTTATATCGCAATGGTTGATAAAAGTGGATATCACGCTAAATTATGA", "aa": "MKFIKRKTKLLTITIGAVAVSSILLGGIFYGTSQKSPSSFGIASIDQKENFINKDNLDYQKARPSIKDNNLKEIPKPKPQPKPEPQPTPFPDPIPTPPKKEELKKPEIKPEEPKKPEIKPEPIPKPKPQPIPQPTPPVETKPKEELLPPSPPPPKEEPKPEPDPQPQPQQIPNQSTVRKIELNGVLVDAEVEVPPPRQTFKYDQDNGLSNLNPYTNISVGKIKKVFVTDELRHKSADLVRGNLKRGDYQSLVKDLLDPNIKPEEIDSYIAMVDKSGYHAKL", "aa_hexdigest": "e21cb4f4292f7f9f0b6f8bf0bd4b057c", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 31479.909499999987, "isoelectric_point": 9.186889839172363 }, "id": "NKIECMJPJD_226", "locus": "GCA000828735_00190" }, { "type": "cds", "sequence": "contig_1", "start": 123416, "stop": 123766, "strand": "-", "frame": 2, "gene": null, "product": "MIP family Ig-specific serine endopeptidase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021E090D9" ], "nt": "ATGGGACTAGAGTATTTACCCAAACATTTTGCACCAGCAGGTGGTGCTTCTGGAACAAGTGTACGCAATCAAAAAAATGAATTGGTTGCTATCTATCATGCTAAATATGATTCATCAAAAACAGGTCTAGCTGCTGCTTTTCGATCTGAAGGTTATGATTATCAAGGATTATATGGAAATTATAATTTACCACAATATGATTTAATTTATGGCGGTGGAAAAGATCAAACCGAAAAGAAATCATACCGAGAAGCGATGAAGGATATATATCAAAATAACAATATTAAAACAGCATTATTTCCTGATGGATTCGATAAGATTCCTGATGAATTCAAATTTAATAATAATTAA", "aa": "MGLEYLPKHFAPAGGASGTSVRNQKNELVAIYHAKYDSSKTGLAAAFRSEGYDYQGLYGNYNLPQYDLIYGGGKDQTEKKSYREAMKDIYQNNNIKTALFPDGFDKIPDEFKFNNN", "aa_hexdigest": "665688d29ae7118b7c75f104c8c1c451", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0021E090D9", "query_cov": 0.9137931034482759, "subject_cov": 0.8548387096774194, "identity": 0.66, "score": 149.0, "evalue": 3e-44, "valid": false, "product": "MIP family Ig-specific serine endopeptidase", "uniref50_id": "UniRef50_UPI0021E090D9" }, "pscc": { "uniref50_id": "UniRef50_UPI0021E090D9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021E090D9" ], "product": "MIP family Ig-specific serine endopeptidase" }, "genes": [], "id": "NKIECMJPJD_227", "locus": "GCA000828735_00191" }, { "type": "cds", "sequence": "contig_1", "start": 124106, "stop": 124240, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGCTAAAGTCATTACAAGTAATTATGCTAATAATAAAAATAACCAGATTAAATTCTTATCTAAATCATATTTAAGTGATTATTCTAAAATTGATGTTCCTTTAAAATATAGACAAGAAGACGCTAAAACATGA", "aa": "MAKVITSNYANNKNNQIKFLSKSYLSDYSKIDVPLKYRQEDAKT", "aa_hexdigest": "ddf295cd4fdfb96e6189deef5b52324a", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5128.768100000002, "isoelectric_point": 9.573829078674315 }, "id": "NKIECMJPJD_228", "locus": "GCA000828735_00192" }, { "type": "cds", "sequence": "contig_1", "start": 124304, "stop": 124579, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01732.22" ], "nt": "ATGCGATTAATGGATTCTGCACAAATTAAAACTACTTTTAGATTAAGTAATTTAGATGAGAATATTTATAATTTTGGTTTTCGAAGTAAAGAACATGGCAAAAATCTTTTAAACCATGGACTTAAAAAAATTTTTGATGGACGTGATTTTTTAAAAACAAAACCGGCTGAATATTTGATTAATTCACAAAAAGAAAAATATAAAGATGTTGGTAATTTTACAGATTTTGCTGTTTTTGAATTAGATTTTGAAAAATTAGAATTAGTTAATGTTTGA", "aa": "MRLMDSAQIKTTFRLSNLDENIYNFGFRSKEHGKNLLNHGLKKIFDGRDFLKTKPAEYLINSQKEKYKDVGNFTDFAVFELDFEKLELVNV", "aa_hexdigest": "db191f73479d1d5de5a570ec21e27b8f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01732.22", "name": "Mycop_pep_DUF31", "length": 87, "aa_cov": 0.9340659340659341, "hmm_cov": 0.9195402298850575, "evalue": 9.894484922089455e-18, "score": 60.40694046020508, "start": 2, "stop": 86 } ], "seq_stats": { "molecular_weight": 10724.114999999993, "isoelectric_point": 7.952191352844237 }, "id": "NKIECMJPJD_229", "locus": "GCA000828735_00193" }, { "type": "cds", "sequence": "contig_1", "start": 124691, "stop": 124801, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCAAATGAAAAATATCGACAAGAAGCAATGCAAACATTTAGTTTAGGAATTTTAAATAACGATTCACACAAGAACAATACTTATAATAAAACTGCAGGTACAACATGA", "aa": "MPNEKYRQEAMQTFSLGILNNDSHKNNTYNKTAGTT", "aa_hexdigest": "7f8453bea47e8aada05645d637e6f22e", "start_type": "TTG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4118.479300000002, "isoelectric_point": 8.161197853088382 }, "id": "NKIECMJPJD_230", "locus": "GCA000828735_00194" }, { "type": "cds", "sequence": "contig_1", "start": 124853, "stop": 125038, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCGCCCTGATTTAAATGCAACTGTTAGTGATATTAAACATTTTGATGAAAAAGCAAAAAACGTGGGTCAAGATTCTTATGAATCTGCTGCTTATAAAGGTTTTACATTACCTGTTTATGAAAGTGATGGCAAAATCAGTGGTTTAGCATTAGCAGGTAAAGATACACCAAAAGGGCCGTCATGA", "aa": "MRPDLNATVSDIKHFDEKAKNVGQDSYESAAYKGFTLPVYESDGKISGLALAGKDTPKGPS", "aa_hexdigest": "f345a6cfbd6793273f40c46a7e0f885b", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6517.160500000002, "isoelectric_point": 5.628390312194824 }, "id": "NKIECMJPJD_231", "locus": "GCA000828735_00195" }, { "type": "cds", "sequence": "contig_1", "start": 125045, "stop": 125920, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF04200.17" ], "nt": "ATGAAAAAAAATAAGTTAACTACTCTCGCTTTAATATTACCAATTACAATTTTAACTCCTATTGTTATTGCCTCATGTACAAATAAAACTAAAGTTAAAAAATCATCATCTTTAGATAAGATTGCTTCAAATTTAAAATTAGAGTATTTTAATAACAAAGCTAATACTAAAGCATCAAGTGTGCAAAAAGATGAAATTAAAAAACCACTAAATTTACCAAATGATGTTGTTTTTAGTGTTAAAGACGTTTTTGTTAGTCACAAAGATCAAAGTGTATTAATTGTCAAATACACTTTAAAAAAAGGGAATGAAATTCAAGAGTATACTTATGAAATTAAAGGTTTTAAATCTGTTTATGAAAAAGATAAAATCGTTAATGATTTAAGTCAAGCAAACGAAGATTTTAAAAAAATTGTTAATAATATCAGGTTAAAAGATACATTTGATTTTAAACTAGCAGCTTTTCCTAATCAAAATTATGACCAATTATTACCATCACAAATTTATAAAAATTATTATCAAGGTATTGAAATTCAACAACACAAATATCAAAATGAATTAGATATTAAAATTATTAATTTTTTATATCCTGATGGTGATTTTGGTTCGGCAAATAAAAATGGTACTTTAAAATTATCATTAATGTTAACTGATAAAAAAAATAATCAAGTTTACTATAAACTTTTAGAGGTTTCTGGCTTTAAATCTAATCCCTATGGTGTTGATGAAAATGGAACAATTCCTGGAATTGGTACAGAACGTTTAAAACCTAAAAATCAAGACGATTATTTTTCAAAAACTCAATTGCAACGTTATGAAATTGATAATGAAGGATATCTTCAAATTTTAAAACGCCAAAATAATGATAAAAATTGA", "aa": "MKKNKLTTLALILPITILTPIVIASCTNKTKVKKSSSLDKIASNLKLEYFNNKANTKASSVQKDEIKKPLNLPNDVVFSVKDVFVSHKDQSVLIVKYTLKKGNEIQEYTYEIKGFKSVYEKDKIVNDLSQANEDFKKIVNNIRLKDTFDFKLAAFPNQNYDQLLPSQIYKNYYQGIEIQQHKYQNELDIKIINFLYPDGDFGSANKNGTLKLSLMLTDKKNNQVYYKLLEVSGFKSNPYGVDENGTIPGIGTERLKPKNQDDYFSKTQLQRYEIDNEGYLQILKRQNNDKN", "aa_hexdigest": "e275eae927ca74b987174e8556dfe92e", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF04200.17", "name": "Lipoprotein_17", "length": 85, "aa_cov": 0.27491408934707906, "hmm_cov": 0.9764705882352941, "evalue": 8.788727042330109e-20, "score": 66.4494857788086, "start": 38, "stop": 117 } ], "seq_stats": { "molecular_weight": 33523.891500000005, "isoelectric_point": 9.283270072937015 }, "id": "NKIECMJPJD_232", "locus": "GCA000828735_00196" }, { "type": "cds", "sequence": "contig_1", "start": 125951, "stop": 126247, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCTATTGAGGACTTAAATGAAGCCGGATTTAACGAACATATTGTAAGTGGTGAACCTGGTGAAAAAAGTAAAATTACTTTTAGCAATGGTTCTGGTACAACTAAAATCCAAATTGATGGTGATCAAGAATTGTCAGCAAATGGAATTAGTAACTTATCAGCAATGTTTAATTTTGCTGAAAGTTTACAACGGACAATTGTTGTAAATAATACCAACTCACCACTTGCAAATCAACTAAGAAATGCGGGATATAGTGTTGAATCAACAACTAACGCTGGACTAATAGATATTTAA", "aa": "MSIEDLNEAGFNEHIVSGEPGEKSKITFSNGSGTTKIQIDGDQELSANGISNLSAMFNFAESLQRTIVVNNTNSPLANQLRNAGYSVESTTNAGLIDI", "aa_hexdigest": "db79684369f4fd386d4bd43f63c8de28", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10406.250799999989, "isoelectric_point": 4.336383247375489 }, "id": "NKIECMJPJD_233", "locus": "GCA000828735_00197" }, { "type": "cds", "sequence": "contig_1", "start": 126677, "stop": 126862, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGGAAATCAGATTCAAGTCAAAAATTTGTTGATATTCTAAAAACATTACCAAATCAATTGCCTTTATTAGAATTGTTTTTCTCCGCTGGTTCGCATAATACCTCTTCATTAATCGCACTGAAGGATAAAAAAATTAAAGAATTAGGTTTGTTCACACTAGGAAATTCATTATTAGATGAATGA", "aa": "MGKSDSSQKFVDILKTLPNQLPLLELFFSAGSHNTSSLIALKDKKIKELGLFTLGNSLLDE", "aa_hexdigest": "8d715ff41db20117057110802f6c0d87", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6692.683799999998, "isoelectric_point": 6.549522972106933 }, "id": "NKIECMJPJD_234", "locus": "GCA000828735_00198" }, { "type": "cds", "sequence": "contig_1", "start": 127166, "stop": 127396, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGTCTAAATTTGTTTCAAAAGACCATAAATATGCATGGCTTTATCAACATTTAGACTTAACAAAGTTTACACAATTATCAAATGAATCAGAATCTTATTTAAAAGAAGGCTATACACCTGATCCTGATAATGCATATGTTGATGAAAATGGAAAAATTAGTTCTCACGCATATTCACCTGCCAAAGGTTATAATTCGGTAACAAGTCGAATGGAAAATGATAATTGA", "aa": "MKSKFVSKDHKYAWLYQHLDLTKFTQLSNESESYLKEGYTPDPDNAYVDENGKISSHAYSPAKGYNSVTSRMENDN", "aa_hexdigest": "296006dccc7dc03af630901c9f4ff0d8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8771.4659, "isoelectric_point": 5.828179359436036 }, "id": "NKIECMJPJD_235", "locus": "GCA000828735_00199" }, { "type": "cds", "sequence": "contig_1", "start": 127574, "stop": 128299, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATTTATTAAACGTAAAACAAAATTATTAACGATTACAATTGGTGCAGTTGCTGTTAGTTCAATCTTATTAGGAGGAATTTTTTATGGTACAAGTCAAAAAAGTCCCTCAAGTTTTGGTATTGCTTCTATTGATCAAAAAGAAAATTTTATTAATAAAGACAATTTAGATTACCAAAAAGCAAGACCATCAATTAAAGATAGTAACTTAAAAGAAATTCCCAAACCTAAACCTCAACCAAAACCTAAGCCTCAACCAACTCCATTTCCAGATCCCATCCCAACACCTCCAAAAAAAGAAGAGCTAAAAAAACCAGATATTAAACCAGAAGAACCTAAAAAACCTGAAATTAAACCAGAACCAAAACCTGAGCCTATTCCTCAACCAGCCCCTCCTATTGAAACAAAACCAAAAGAAGAATTATTACCACCCAACCCTCCGCCTCCAAAAGAAGAACCAAAACCAGAACCAAATCCGCAACCACAGCCACAACAGGTTCCAAACAATAGTAATTCACGAATTATAGAAATTAATGGTGTAAGAGTGGAAGCTGAAGTTGAAGTCCCTCCCCCTCGTGATATTGCTGAATATGATAAACAAAATAATCTGGTTAACCCTAATCCATACATTAATGATAGTGTTGGTAAAATTAAAAACGTAAAGGTTACCGATGAATTGCGAAAAGCAACAGGTAAATTGGTCCAAGGAAATTTAGGACGTTGA", "aa": "MKFIKRKTKLLTITIGAVAVSSILLGGIFYGTSQKSPSSFGIASIDQKENFINKDNLDYQKARPSIKDSNLKEIPKPKPQPKPKPQPTPFPDPIPTPPKKEELKKPDIKPEEPKKPEIKPEPKPEPIPQPAPPIETKPKEELLPPNPPPPKEEPKPEPNPQPQPQQVPNNSNSRIIEINGVRVEAEVEVPPPRDIAEYDKQNNLVNPNPYINDSVGKIKNVKVTDELRKATGKLVQGNLGR", "aa_hexdigest": "daaa1aa9b0b4f871daeb346dca705889", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 26798.55, "isoelectric_point": 9.439605903625488 }, "id": "NKIECMJPJD_236", "locus": "GCA000828735_00200" }, { "type": "cds", "sequence": "contig_1", "start": 128322, "stop": 128813, "strand": "-", "frame": 1, "gene": null, "product": "MIP family Ig-specific serine endopeptidase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021DFAD80" ], "nt": "TTGTCTTATGCGATTGGTTACCGTAGTTTTATCGATAAACCTGGTGTTCTAGATGCCTTTATTTCTGCTCCTCATACTGGCAATGATTTATATAAATCTCTTGATGGAAAAAAATATATTAATATGGGTTTGGAATATATGCCTAGACATTATGCTCCAGCAGGAGGTGCATCTGGTTCAAGTGTGCGAAACCAAAACAATGAAATTGTAGGAATTTATCATGTAAGTAATGAATTTGCTAGCACCGGCTTAGCAACTGCTTTTCGTTCTGAAGGATATGATTATAAAGGCTTATATGGTGATAAATACAAAGAATTACCACAATATGACTTAATTTATGGCGGTGGAAAAGATCAAGCTACAGGTCAATCATATCGTGAAAAAATGAAAGAAATATATAAAAATAATAACAATATTAAAACAGCATTATTTCCAAAAGGTTTTGACCAATATGATGAAAAGTTTAAATTTAATAACAATCAAAGTAAATAA", "aa": "MSYAIGYRSFIDKPGVLDAFISAPHTGNDLYKSLDGKKYINMGLEYMPRHYAPAGGASGSSVRNQNNEIVGIYHVSNEFASTGLATAFRSEGYDYKGLYGDKYKELPQYDLIYGGGKDQATGQSYREKMKEIYKNNNNIKTALFPKGFDQYDEKFKFNNNQSK", "aa_hexdigest": "bca9556c9c8f17fd28b7243b45dc269d", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002AFE92B4", "query_cov": 0.9570552147239264, "subject_cov": 0.9629629629629629, "identity": 0.596, "score": 176.0, "evalue": 6.82e-54, "valid": false, "product": "MIP family Ig-specific serine endopeptidase", "uniref50_id": "UniRef50_UPI0021DFAD80" }, "pscc": { "uniref50_id": "UniRef50_UPI0021DFAD80", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021DFAD80" ], "product": "MIP family Ig-specific serine endopeptidase" }, "genes": [], "id": "NKIECMJPJD_237", "locus": "GCA000828735_00201" }, { "type": "cds", "sequence": "contig_1", "start": 129216, "stop": 129488, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01732.22" ], "nt": "ATGAAATTAATGGATAGTGTTAAAACTAAAACAACTTTACGTTTAAGTAATTTAGATGAAAATATTTATCGTTTTGGTTTTGCAAGTAAAAATAACGAATTTTTATTAAATCATGGTCTTAAAAAAATATATGATGGTCGAGATTTCTTAAAAACTAAACCTACAGAATACTTAACAGAACAACAAAAAGAAAAATACAAAGATGCAGGTAGTTTTGTTGATTTTGCAGTTTTTGAATTAGATTTCGAACAATTAAAATTACTTAATGTTTGA", "aa": "MKLMDSVKTKTTLRLSNLDENIYRFGFASKNNEFLLNHGLKKIYDGRDFLKTKPTEYLTEQQKEKYKDAGSFVDFAVFELDFEQLKLLNV", "aa_hexdigest": "0964c6dea1094469355d97bf0b276120", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01732.22", "name": "Mycop_pep_DUF31", "length": 87, "aa_cov": 0.9444444444444444, "hmm_cov": 0.9310344827586207, "evalue": 9.552903962701652e-18, "score": 60.457115173339844, "start": 2, "stop": 86 } ], "seq_stats": { "molecular_weight": 10620.042199999994, "isoelectric_point": 7.901583671569825 }, "id": "NKIECMJPJD_238", "locus": "GCA000828735_00202" }, { "type": "cds", "sequence": "contig_1", "start": 129762, "stop": 129947, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCGTCCTGAACTTGGTGATAATGAACAAAAAATTAAAGAATTTGATGAAAAAGCTAAAGGTGTAAGTCAAGATGCTTATGCGTCTGCTGCTTATAAAGGTTTTACACTCCCAGTGTATGATAAAAATGGTGATTTTAAAGGTTTATCAATTAATGAAAGTAGTTACGGCCAAGCTCGTTCATGA", "aa": "MRPELGDNEQKIKEFDEKAKGVSQDAYASAAYKGFTLPVYDKNGDFKGLSINESSYGQARS", "aa_hexdigest": "8ce0faeb40858169b8b1303249d40cc2", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6751.328700000001, "isoelectric_point": 5.2417715072631825 }, "id": "NKIECMJPJD_239", "locus": "GCA000828735_00203" }, { "type": "cds", "sequence": "contig_1", "start": 129954, "stop": 130838, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF04200.17" ], "nt": "ATGAAAAAGTTGAAATTAATTACTTTAAGTTTATCACCTATTCCTATTGTCGCGATCATGGCTACAGCATGTAGTACAACAAAAAAGGAAAAAGAAGTAGATCAAAAACAAGATGAATTAACTACTATAAATAGTAATTTACAATTAGAATATCCCCAAAACAAAGATATAGAAACATCAGCAGCAATAAAGGAAGAAATAAAGCCTTTAAATTTGCCGTCTGGTGTTGTTTTTAGCGTTAAAGAAATTAGTCCTAAAGTAGATGATGAAAGTACACTAATAGTTAAATACACCCTTAAAAAGGGAAACATAACTAAAGAATTTAAAAAAGAAATTAATGGATTTAAAAAAATTCGTAAAGATCAAAATATCGCTAATATAAATGACATGGATCATGATTTTAAAACGATTTTTGATAACATTAAATTAAAAGATACATTTGATTTTAAACTAAAAGGTCTTTTTAACTCAAATTTTGGTGAATACGATAAATTATTACCCTCACAAATTGTTAACAATTTTGCACAAGGTGTAGAAACTAGAATTAATACTAACCAAGATCGTTTAGGTATTGAAGTTATAGATGTTAGTTATCCAAACGAAAATTTTGGTTCTGCTAATCGTGAAGGTAATTTAAAAATGTCACTATTAGTAACTGATAAAAAAACAAAACAAGTTTTCATTAAATCAATAATTGCGTATGGTTTTAAAACTAATGCAATGGGTTTAGATGAAAATGGTTCAATTCCAGGTGGTGGTGCTGATCTTGTTAAACCTAAAGTTGATGAAAATAATTATTTTAATAAAACTCAATTAGAAAGATATGAAATTGATAATCAAGCTTATTTAAATGGTTTAAAAGGCCAACATATGGGTAAAACTTGA", "aa": "MKKLKLITLSLSPIPIVAIMATACSTTKKEKEVDQKQDELTTINSNLQLEYPQNKDIETSAAIKEEIKPLNLPSGVVFSVKEISPKVDDESTLIVKYTLKKGNITKEFKKEINGFKKIRKDQNIANINDMDHDFKTIFDNIKLKDTFDFKLKGLFNSNFGEYDKLLPSQIVNNFAQGVETRINTNQDRLGIEVIDVSYPNENFGSANREGNLKMSLLVTDKKTKQVFIKSIIAYGFKTNAMGLDENGSIPGGGADLVKPKVDENNYFNKTQLERYEIDNQAYLNGLKGQHMGKT", "aa_hexdigest": "33ba90295f09102d5d10022566d97744", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF04200.17", "name": "Lipoprotein_17", "length": 84, "aa_cov": 0.2653061224489796, "hmm_cov": 0.9880952380952381, "evalue": 7.319555745405658e-10, "score": 34.656959533691406, "start": 40, "stop": 117 } ], "seq_stats": { "molecular_weight": 33125.479799999994, "isoelectric_point": 8.596230506896976 }, "id": "NKIECMJPJD_240", "locus": "GCA000828735_00204" }, { "type": "cds", "sequence": "contig_1", "start": 131024, "stop": 131440, "strand": "-", "frame": 2, "gene": "sWEET", "product": "putative protein UU042", "db_xrefs": [ "COG:COG4095", "COG:G", "GO:0005886", "RefSeq:WP_006688624.1", "SO:0001217", "UniParc:UPI000169E457", "UniRef:UniRef100_A0AAC9X7R2", "UniRef:UniRef50_Q9PRA3", "UniRef:UniRef90_Q9PRA3" ], "nt": "ATGTCGCCAATTAGTATTGAATTAATAATTCAAATAAGTATTGGATTAAGTGCTAGCCTAATTTTATTGTTTGCTTTCTTACCACAAACATTTTTAACTATTAAAACTAAAAACACCGCTGCATTAACAATTAGTATGTTTATTATTTGTTTTATTGCACGTTTATGTTTTAGTTTAAGTGCTATTCTAACAATTATCATTTATATTCATAATCAGGATTATGGCTTATCTTTATACGCGCTTACACTTCCTGTTTTAATATGTCATGGCATTAATATGTTATTAAATTTAATTATTGCTTTTATAAAAATCAATAATGTTTATAAAGCTAAAATCCATAAAATGAATGAGAATGAATATATTGCTTTCGCTTATGCTCAAAAATTAAAGAAAAAAGTATTGATAAAAAACAAATAA", "aa": "MSPISIELIIQISIGLSASLILLFAFLPQTFLTIKTKNTAALTISMFIICFIARLCFSLSAILTIIIYIHNQDYGLSLYALTLPVLICHGINMLLNLIIAFIKINNVYKAKIHKMNENEYIAFAYAQKLKKKVLIKNK", "aa_hexdigest": "8c0e0507eba9c6d6401d3c1c5fe75e32", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000169E457", "ncbi_nrp_id": "WP_006688624.1", "uniref100_id": "UniRef100_A0AAC9X7R2", "db_xrefs": [ "SO:0001217", "UniParc:UPI000169E457", "RefSeq:WP_006688624.1", "UniRef:UniRef100_A0AAC9X7R2" ] }, "ips": { "uniref100_id": "UniRef100_A0AAC9X7R2", "uniref90_id": "UniRef90_Q9PRA3" }, "psc": { "uniref90_id": "UniRef90_Q9PRA3", "gene": "SWEET", "product": "Uncharacterized protein UU042", "uniref50_id": "UniRef50_Q9PRA3", "cog_id": "COG4095", "cog_category": "G", "go_ids": [ "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PRA3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PRA3" ], "product": "Uncharacterized protein UU042" }, "genes": [ "sWEET" ], "id": "NKIECMJPJD_241", "locus": "GCA000828735_00205" }, { "type": "cds", "sequence": "contig_1", "start": 131440, "stop": 131802, "strand": "-", "frame": 3, "gene": "sWEET", "product": "putative protein UU041", "db_xrefs": [ "COG:COG4095", "COG:G", "GO:0005886", "RefSeq:WP_006688549.1", "SO:0001217", "UniParc:UPI000169E458", "UniRef:UniRef100_A0AAC9X6W9", "UniRef:UniRef50_Q9PRA4", "UniRef:UniRef90_Q9PRA4" ], "nt": "ATGAGTTCATTACCAGTTATTATTTTAGTAATTGGCATTTTTGGGTCTATTTTTCTAGTAATAGGATATATTCCCCAAGTAATTAAAGTTATCAAAACAAAACGAACAGACGGAATCTCATTAACATTTTTAATTTCTTTAAACATTGCTTGTTTTCTATTTGTAATATACAGTATATTAGTAATGATTTTTAACAGACACAATGGTATTCCTACTGCTTTACCATTGTGTCTTGCAAACACCATAGTAGGAATTTTAGGTCTAGTTATTTTAATTTATAAAGTTAAAAACATTAAAAAAGCTAAATTATATTTAATAGATGAAAAGACATATTATGAAAAATATGTCTTAAATAATTTATAA", "aa": "MSSLPVIILVIGIFGSIFLVIGYIPQVIKVIKTKRTDGISLTFLISLNIACFLFVIYSILVMIFNRHNGIPTALPLCLANTIVGILGLVILIYKVKNIKKAKLYLIDEKTYYEKYVLNNL", "aa_hexdigest": "1db2475149f70bee016ef7e53ce547b5", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI000169E458", "ncbi_nrp_id": "WP_006688549.1", "uniref100_id": "UniRef100_A0AAC9X6W9", "db_xrefs": [ "SO:0001217", "UniParc:UPI000169E458", "RefSeq:WP_006688549.1", "UniRef:UniRef100_A0AAC9X6W9" ] }, "ips": { "uniref100_id": "UniRef100_A0AAC9X6W9", "uniref90_id": "UniRef90_Q9PRA4" }, "psc": { "uniref90_id": "UniRef90_Q9PRA4", "gene": "SWEET", "product": "Uncharacterized protein UU041", "uniref50_id": "UniRef50_Q9PRA4", "cog_id": "COG4095", "cog_category": "G", "go_ids": [ "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PRA4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PRA4" ], "product": "Uncharacterized protein UU041" }, "genes": [ "sWEET" ], "id": "NKIECMJPJD_242", "locus": "GCA000828735_00206" }, { "type": "cds", "sequence": "contig_1", "start": 131927, "stop": 132151, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCGTGCTGAAGATTTAACACAATCGATGCGTGAATCTTTTGATGTCGCAACTTCAAGAGCTGTTGCGTCATTAGATAAAATTTTAGAAATTTCAGCCGCATTTGTTAAAGTTAATGGTTATTTAATTCAACCAAAATCAACAAAATTTTATCAAGAAGAACTAAAAGCAAAAAATATTATAAAAACTTTGTATTTAGAAAGAATTGCTTTAAAAGATTTTTGA", "aa": "MRAEDLTQSMRESFDVATSRAVASLDKILEISAAFVKVNGYLIQPKSTKFYQEELKAKNIIKTLYLERIALKDF", "aa_hexdigest": "5d7af2a3b281eb1ab34c2d36909515f0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8486.769199999995, "isoelectric_point": 9.002897071838383 }, "id": "NKIECMJPJD_243", "locus": "GCA000828735_00207" }, { "type": "cds", "sequence": "contig_1", "start": 132155, "stop": 132415, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGACAAGACTTGATAGTGATGATAAGATTTACCAAAATTTCTTTTTAGATTCACTTGCGCCTTATAAAGAACTAGATTTTTTTACACAAAACACTAATCTTAAATTAATTGATATTGGTTCTGGTTCTGGAATTCCAGGGGTTGTTTTAAAGATTATTTTTAAAAATTTAAATCTAACATTATTAGAAGCTAATCAAAAACGATGTGAATTTCTTAAAATATTAACTCAAGAATTAGGTTTAAATGATGTTTTAATTTGA", "aa": "MTRLDSDDKIYQNFFLDSLAPYKELDFFTQNTNLKLIDIGSGSGIPGVVLKIIFKNLNLTLLEANQKRCEFLKILTQELGLNDVLI", "aa_hexdigest": "45ad68047371fd10ae618dd1a704d5d5", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9793.270499999991, "isoelectric_point": 4.886016273498535 }, "id": "NKIECMJPJD_244", "locus": "GCA000828735_00208" }, { "type": "cds", "sequence": "contig_1", "start": 132942, "stop": 134030, "strand": "-", "frame": 1, "gene": "mnmG", "product": "tRNA uridine-5-carboxymethylaminomethyl(34) synthesis enzyme MnmG", "db_xrefs": [ "COG:COG0445", "COG:J", "GO:0002098", "GO:0005829", "GO:0008033", "GO:0030488", "GO:0050660", "SO:0001217", "UniRef:UniRef50_Q6MRU5", "UniRef:UniRef90_Q9PRA6" ], "nt": "TTGGATTTAATTATTGCTGAAGTTAGTGATCTATTAGTAGAAAATAACATTATTAAAGGTGTTATTTTAAGTGATCAAAAAATCATTTATGCTAATTATGTGATTATTACAACAGGAACTTATTTAAAATCAATTACTCATCGAGGTTCAGTTTGTGTTGATGAAGGAGCAGATGGTACAAAAAATGCAAAATTTTTAAGTGATGCATTAGTAAAATTAGGATTTAAGTTAATTCGTTTAAAAACAGGGACTCCTGCAAGAATTAAAAAAGATTCGATTGATTTTACAAACATGATTCTTGAACCTGGCACAAATCAAAAAATTGCTTTTAGTCACTATCACCCTGTTTATAAACCTTATGACGAACAACTTCCTTGTCATATTATTTATACAAACGAACAAACTCACCAAATTATTCGAGAAAATTTAAATAAATCTGCAATGTATGGGGGTATGATTTCTGGAATTGGGCCCCGTTATTGCCCTTCAATTGAAGATAAAATTGTTAAATTTAGTGAAAAACCGCGTCACCAAATTTTTGTTGAACCAGAATCTTATGAATTAGATTCAATGTACTTAGGGGGTTTTTCTACTTCAATGCCAATCGATGTTCAAGAAAAAATGATCCGTAGTTTACCAGGATTAGAAAGTTGTAAAATTTTAAAATACGCTTATGCTATTGAATATGATGCTATTGATCCGACCCAACTATATCCTAGTTTAGAATCGAAATTAGTAAAAAATTTATTTTTTGCTGGTCAAATCAATGGAACTAGTGGATATGAAGAAGCCGCAGCACAAGGATTGATGGCAGCAATTAATGTTAATCAAAAATATCAAAATAAAGAACCTGTTATTTTAGGTCGTGATCAAGCATATATTGGTGTTATGATTGATGATATTGTTACTAAGGGCGTTGTCGAACCATATCGTTTACTAACAAGCCGTGCTGAACACCGGTTAGCATTAAGAAATGATAATGCTGATGATCGATTAATGAAAATTGGTTTTGAAATTGGTTTATTAAAATCTGAAGTATATGACCAATATTTAAATAATTTAAAACAAATTAAAGAAATCTTAAACTGA", "aa": "MDLIIAEVSDLLVENNIIKGVILSDQKIIYANYVIITTGTYLKSITHRGSVCVDEGADGTKNAKFLSDALVKLGFKLIRLKTGTPARIKKDSIDFTNMILEPGTNQKIAFSHYHPVYKPYDEQLPCHIIYTNEQTHQIIRENLNKSAMYGGMISGIGPRYCPSIEDKIVKFSEKPRHQIFVEPESYELDSMYLGGFSTSMPIDVQEKMIRSLPGLESCKILKYAYAIEYDAIDPTQLYPSLESKLVKNLFFAGQINGTSGYEEAAAQGLMAAINVNQKYQNKEPVILGRDQAYIGVMIDDIVTKGVVEPYRLLTSRAEHRLALRNDNADDRLMKIGFEIGLLKSEVYDQYLNNLKQIKEILN", "aa_hexdigest": "87c0b2114c8b8493756dbd7c197fbf3c", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A920QIU6", "query_cov": 0.8093922651933702, "subject_cov": 0.966996699669967, "identity": 0.5670000000000001, "score": 317.0, "evalue": 4.35e-104, "valid": false, "product": "tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG", "uniref50_id": "UniRef50_A0A800JDM4", "kegg_orthology_id": "K03495" }, "genes": [ "mnmG" ], "truncated": "both", "pseudogene": { "start": 132639, "stop": 134330, "observations": { "mutation": [ 132944, 134063, 134105 ], "stop": [ 132944, 134063, 134105 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PRA6", "query_cov": 1.0, "subject_cov": 0.5895765472312704, "identity": 0.972, "score": 698.0, "evalue": 9.98e-250, "gene_start": 1, "gene_end": 362, "reference_start": 115, "reference_end": 476, "reference_sequence": "MKKYDVIVIGAGHAGLEAAFATSNLNLQTALITLDEKGIGMMPCNPSIGGPAKGIVTREIDALGGIQGKAADATTMQMKILNSSKGPGVWAIRAQIDKIAYQRWFKQQIKQQKNLDLIIAEVSDLLVENNIVKGVVLSDQKIIQADYVIITTGTYLKSITHRGSVCVDEGADGTKNAKFLSDALVKLGFELIRLKTGTPARIKKDSIDFTNMILEPGTNQKIAFSHYHPVYKPYDEQLPCHIIYTNEQTHQIIRENLNKSAMYGGMISGIGPRYCPSIEDKIVKFSEKPRHQIFVEPESYELDSMYLGGFSTSMPIDVQEKMIRSLPGLENCEILKYAYAIEYDAIDPTQLYPSLESKLVNNLFFAGQINGTSGYEEAAAQGLMAAINVNQKYQNKEPVILGRDQAYIGVMIDDIVTKGVVEPYRLLTSRAEHRLALRNDNADDRLMKIGFEIGLLKPEVYDQYLNNLKQIKEILNWLKTTTVGQIDDLKFTTLKTNSYLIDYLKRPEIKLNDLLIYCPIKIEDEQIINKVQIQVKFEGYIKNQEENLKQLKRLNNIKLHAIVDYKEVPNISLETIDKLNKIKPLDLEQASRISGVNLTDIAMIKYYLERIKND" }, "paralog": false, "identity": 0.9769094138543517, "score": 1083.0, "evalue": 0.0, "description": "Internal stop codon at 132944, 134063, 134105. Nonsense mutation around 132944, 134063, 134105.", "psc": { "uniref90_id": "UniRef90_Q9PRA6", "gene": "mnmG", "product": "tRNA uridine-5-carboxymethylaminomethyl(34) synthesis enzyme MnmG", "uniref50_id": "UniRef50_Q6MRU5", "cog_id": "COG0445", "cog_category": "J", "go_ids": [ "GO:0002098", "GO:0005829", "GO:0008033", "GO:0030488", "GO:0050660" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PRA6", "UniRef:UniRef50_Q6MRU5", "COG:COG0445", "COG:J", "GO:0002098", "GO:0005829", "GO:0008033", "GO:0030488", "GO:0050660" ] }, "pscc": { "uniref50_id": "UniRef50_Q6MRU5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q6MRU5" ], "product": "tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG" } }, "id": "NKIECMJPJD_245", "locus": "GCA000828735_00209" }, { "type": "cds", "sequence": "contig_1", "start": 134103, "stop": 134372, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF07992.20" ], "nt": "GTGAAAAAATATGATGTAATTGTCATTGGTGCTGGACACGCAGGATTAGAAGCAGCTTTTGCAACTTCTAATCTTAATTTACAAACAGCATTAATTACATTAGATGAAAAAGGGATTGGTATGATGCCATGCAATCCTTCAATAGGAGGACCGGCTAAAGGAATTGTTACACGTGAAATTGATGCCCTTGGTGGTATTCAAGGTAAGGCAGCTGATGCTACAACAATGCAAATGAAAATCTTAAATTCATCAAAAGGACCTGGTGTTTGA", "aa": "MKKYDVIVIGAGHAGLEAAFATSNLNLQTALITLDEKGIGMMPCNPSIGGPAKGIVTREIDALGGIQGKAADATTMQMKILNSSKGPGV", "aa_hexdigest": "d6186cc091bff2597a6971512abb283a", "start_type": "GTG", "rbs_motif": "AGxAGG/AGGxGG", "psc": { "uniref90_id": "UniRef90_UPI002852D7CD", "query_cov": 0.9662921348314607, "subject_cov": 0.8269230769230769, "identity": 0.593, "score": 108.0, "evalue": 3.82e-29, "valid": false, "product": "FAD-dependent oxidoreductase", "uniref50_id": "UniRef50_A0A967GLJ3" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07992.20", "name": "Pyr_redox_2", "length": 50, "aa_cov": 0.5617977528089888, "hmm_cov": 0.98, "evalue": 1.0009572133034026e-07, "score": 27.2376708984375, "start": 4, "stop": 53 } ], "seq_stats": { "molecular_weight": 9040.468699999994, "isoelectric_point": 7.879728889465332 }, "id": "NKIECMJPJD_246", "locus": "GCA000828735_00210" }, { "type": "cds", "sequence": "contig_1", "start": 134468, "stop": 134827, "strand": "-", "frame": 2, "gene": null, "product": "HTH domain protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A133KCP2" ], "nt": "ATGAATTTAATGGAAAGAAGAGGTTCTGGTTTAAAAAATATTTTAGAAATTTATGCATCTAAAGAAAATTATCGAGAAGATTTAAAACCGGAGTTTCATTCAACCGAATCTGCTTTCTTTGTTGTTTTAAAAAATTTAAATTATCAAGAGCAAAATATATCAAAAAATGAAAATCAAAAAATATCCCCTAGCCAAAGACGACAAAAAATCGTGGAATTTATCGAACAAAATCCAAAAACAACAGCTTATGAATTAACAAAAGTATTTTTTGTTTCAATTAATACTATTGAACGTGATCTAGCTAAATTAGTACATGATGGATATGTTGAATTTGTGGGATCATCAAAAAATGGTGAATGA", "aa": "MNLMERRGSGLKNILEIYASKENYREDLKPEFHSTESAFFVVLKNLNYQEQNISKNENQKISPSQRRQKIVEFIEQNPKTTAYELTKVFFVSINTIERDLAKLVHDGYVEFVGSSKNGE", "aa_hexdigest": "8c7a58d5bb07b572bed01fe0a4fb0ee2", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI003398ADF9", "query_cov": 1.0252100840336134, "subject_cov": 0.8299319727891157, "identity": 0.5329999999999999, "score": 122.0, "evalue": 1.46e-33, "valid": false, "product": "ATP-binding protein", "uniref50_id": "UniRef50_A0A133KCP2" }, "pscc": { "uniref50_id": "UniRef50_A0A133KCP2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A133KCP2" ], "product": "HTH domain protein" }, "genes": [], "id": "NKIECMJPJD_247", "locus": "GCA000828735_00211" }, { "type": "cds", "sequence": "contig_1", "start": 134870, "stop": 135028, "strand": "-", "frame": 2, "gene": null, "product": "ATP-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00030D0A09" ], "nt": "GTGCAAGAAGTAATAGTTAATGCTTTAATTCATCGAGATTATTCAATTATTGGTAGTGAAGTCCATATTGACATGTATGATGATAGATTAGAAGTATATTCTCCTGGTGGTATGTTTGATGGGAATTTTGTCCAAAATATTGACCCTTATAATGTATGA", "aa": "MQEVIVNALIHRDYSIIGSEVHIDMYDDRLEVYSPGGMFDGNFVQNIDPYNV", "aa_hexdigest": "3e7d64eaafc05add4c85340bba0caf03", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00030D0A09", "query_cov": 1.0, "subject_cov": 0.8125, "identity": 0.519, "score": 65.9, "evalue": 3.29e-13, "valid": false, "product": "ATP-binding protein", "uniref50_id": "UniRef50_UPI00030D0A09" }, "pscc": { "uniref50_id": "UniRef50_UPI00030D0A09", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00030D0A09" ], "product": "ATP-binding protein" }, "genes": [], "id": "NKIECMJPJD_248", "locus": "GCA000828735_00212" }, { "type": "cds", "sequence": "contig_1", "start": 135203, "stop": 135364, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAAAAAATGAATATCAAAAACATACAGAAAATTTATGGGAGGATAAATTTTTAAAATCCTTTGAATTAATTGATAACAATAATTACTTAACAAATGCTGGAGCTTTATTTGCTGATGGATATCAAGTTTATCAATCACGAGTTTTTTGTACTCGTTGA", "aa": "MLKNEYQKHTENLWEDKFLKSFELIDNNNYLTNAGALFADGYQVYQSRVFCTR", "aa_hexdigest": "e7f3311f2611cf6530d6dee7915e1293", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6368.041200000002, "isoelectric_point": 5.61679515838623 }, "id": "NKIECMJPJD_249", "locus": "GCA000828735_00213" }, { "type": "oriC", "sequence": "contig_1", "start": 135839, "stop": 135930, "strand": "?", "product": "origin of replication", "nt": "CAAAATCTCCTTGTATTATTTACAAGTCATTATACTACTAATATTGTTATTAAAATTTTGGATAAATACTAAATTATTGTGACTAATAAAAT", "id": "NKIECMJPJD_1259" }, { "type": "cds", "sequence": "contig_1", "start": 135931, "stop": 136320, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01420.25" ], "nt": "ATGTATGATGGCGAATTTATTACAATAAGTGCTGATGGAGCTTATGCTGGAACTGTATTTTTACAAAATGGAAAATTTTCTATAACTAACGTTTGTTTTATACTAATAAAAAATAAATATATTGATTTTAAATTTAATAATAAATTTGTTTACTACATTCTTAAGAAAGAACAAGAAATAAATAGACTTAAATCACAAGTTGGTTCTTCTAGACCTGCTGTACGAGAATATTCATTAAAAGAAATTAAAATTAATCTACCTAATATGGAAATTCAAGAAGAATTTTCTAAAATTGTCGAACCACTACTAAATTTGTCTACAAAAGCTAATAAAATTGAAAAAATTTTAAATGACTCTTTATTAAAAATTACTAAAAAACTAATAGTTTAA", "aa": "MYDGEFITISADGAYAGTVFLQNGKFSITNVCFILIKNKYIDFKFNNKFVYYILKKEQEINRLKSQVGSSRPAVREYSLKEIKINLPNMEIQEEFSKIVEPLLNLSTKANKIEKILNDSLLKITKKLIV", "aa_hexdigest": "e01a9e0ea03a4b481b528d137eadc205", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01420.25", "name": "Methylase_S", "length": 115, "aa_cov": 0.875968992248062, "hmm_cov": 0.9478260869565217, "evalue": 2.101255739309448e-17, "score": 59.3997688293457, "start": 3, "stop": 115 } ], "seq_stats": { "molecular_weight": 14903.29519999999, "isoelectric_point": 9.3617280960083 }, "id": "NKIECMJPJD_250", "locus": "GCA000828735_00214" }, { "type": "cds", "sequence": "contig_1", "start": 136465, "stop": 136770, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGTACGTAAATTTAATGATTATGATTTTTCACAATTCAATGAGGATTTAGTAGGAAGAATTTATGAATATTTTTTAGGCGAATTCTTTCGTAAACAAGGGCAAAAAGGTGGAGAATTTTATACACCAAAAACAGTTGTAGAATTATTAATTGATATTTTGGATCCAAACGATAATATTAAAATGTACGATCCTGCTTGTGGAACAGGTGGTATGTTTGTTCAAGCGCGTAATTACTTACATGAACAAAACAAAGATTATAATAAATTAGTTATTTATGGCCAAGAATACCAATCACAAACTTGA", "aa": "MVRKFNDYDFSQFNEDLVGRIYEYFLGEFFRKQGQKGGEFYTPKTVVELLIDILDPNDNIKMYDPACGTGGMFVQARNYLHEQNKDYNKLVIYGQEYQSQT", "aa_hexdigest": "9c8584b3fd020069f651474a902dabda", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11946.243099999994, "isoelectric_point": 4.860381889343262 }, "id": "NKIECMJPJD_251", "locus": "GCA000828735_00215" }, { "type": "cds", "sequence": "contig_1", "start": 137245, "stop": 137568, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGCGAGTTAAAAACTAAAAAATTACGTTTTTTAACTAAAGATGACATTTTAAAAATTAAGAATGTTTATGATCAACATGAACAAGGTGAAGACGTCAATGTTGTAGGATTTGCTAAAACTTGTACAATTGATGAAATTATTGAAAATGATTATTCACTAGTTCCTGGACGTTATGTTGGTTTTGAACAAGAAGTGATTGACCATGAACAATTAAATCACGAAATTAAAGAATTAAAAGAAGAGTTATCAGTTTTATTTGATGAACTTGAAGATTTAATTCCTCAGGCCAAAGAGTCAATTAAAAAAGTATTAGAAAAATAA", "aa": "MGELKTKKLRFLTKDDILKIKNVYDQHEQGEDVNVVGFAKTCTIDEIIENDYSLVPGRYVGFEQEVIDHEQLNHEIKELKEELSVLFDELEDLIPQAKESIKKVLEK", "aa_hexdigest": "3cc9282b63a306e308f871b183b50224", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12475.043999999994, "isoelectric_point": 4.726526069641112 }, "id": "NKIECMJPJD_252", "locus": "GCA000828735_00216" }, { "type": "cds", "sequence": "contig_1", "start": 137904, "stop": 138017, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTTATAATGAATTCAAAGAACATAAATATAATAGTATTCCTAAAATAAATGATTTTAATGAACTAGAATATTTTAATTTAGATACTATTGATATATTGAATCAAGTTTGA", "aa": "MLYNEFKEHKYNSIPKINDFNELEYFNLDTIDILNQV", "aa_hexdigest": "91c27eee2901ca0c9b8db3996783b6f7", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4566.059700000002, "isoelectric_point": 4.433748435974121 }, "id": "NKIECMJPJD_253", "locus": "GCA000828735_00217" }, { "type": "cds", "sequence": "contig_1", "start": 138190, "stop": 138297, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCTAAGAAAATAAATAACAATAAAACTCCTCGTAATAAAGTTAAAAATAACAATGTTTCAAAAGATAATGATGACATTGTTATTTCTTTGCGTTATGAGAATTGA", "aa": "MSKKINNNKTPRNKVKNNNVSKDNDDIVISLRYEN", "aa_hexdigest": "142e6c0733274ab8e03bbab82b3baf06", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4104.563700000001, "isoelectric_point": 9.871673011779784 }, "id": "NKIECMJPJD_254", "locus": "GCA000828735_00218" }, { "type": "cds", "sequence": "contig_1", "start": 139096, "stop": 139488, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000320AE61" ], "nt": "TTGCGGTTTTCATTAATTAATAAAATTTTAGCAATTATACTATCTTATATTTTTTGCATAAGTATTTATCGTTTTCGTAAAAAATTAGTACAATATAAAAATTTTAATGAAATAAAATCGATTAAAAATAATGCGATTCTTATTGTAATCTCTAGTATTGTTACACTACTTTTATTAACACTATTTATTAATTTAATGATTGTTTTTATTAATCAAAATTACTATTACATTAAACGTATTCATAATTTACTTATACCAGTAAGAATTAATGAAATTAAAATTTTAGAATATAGTTACATTTATTTATTACGTTGTGGATTTTATATTTTATTAACAATATTTATAGCAAAATTTGTTCATGATAAAAGTTTTATCATTATATTTATTTATTAA", "aa": "MRFSLINKILAIILSYIFCISIYRFRKKLVQYKNFNEIKSIKNNAILIVISSIVTLLLLTLFINLMIVFINQNYYYIKRIHNLLIPVRINEIKILEYSYIYLLRCGFYILLTIFIAKFVHDKSFIIIFIY", "aa_hexdigest": "08440bf2062b5424062c751bac717492", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI000320AE61", "query_cov": 1.0, "subject_cov": 0.8441558441558441, "identity": 0.892, "score": 208.0, "evalue": 4.84e-67, "valid": false, "uniref50_id": "UniRef50_UPI000320AE61" }, "pscc": { "uniref50_id": "UniRef50_UPI000320AE61", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000320AE61" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15687.020799999986, "isoelectric_point": 9.890497779846193 }, "id": "NKIECMJPJD_255", "locus": "GCA000828735_00219" }, { "type": "cds", "sequence": "contig_1", "start": 139955, "stop": 140458, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01732.22" ], "nt": "TTGCCACAATTACAACTATTTAGTGGAGGATATCCTGGTGATGTTAATCCTAATAGTTCAGCGATTGTTTCATGGCGCGGTTCTAAATCTTATGGTTCACTAATTCAAGCATTTGATCGTGAAATTAAAAATGAATCAATTTTAGATTATTATGGTCCAAAGCAAATTAATAATATTGATGGTTATCAAAAGGTTGGTGAAGGTTATTTAAATAAATTATTTAATGTTGGAACACGAGTTATTACCTCTGATGAAATAGGTGATTTGGGTTCTGGTTCATCTGGTTCAATGATTATTGATTCTAACTTTAATTTAGTAGGTATTCATTTTGCTTCTTTAAATTCGCGTGCTTATGGTGCGCCAAATGATTCAATGATCGGTAATTTATTTGTTGCTCAATCCCAAGACTTAAGTGGTGATATTGATGTAAGAGCTGCGGTGATAAAAAAACTAAAAGCTCAAAATATTTACACTTATAAACTAAACCCTAAAGTTAGTTCTTAA", "aa": "MPQLQLFSGGYPGDVNPNSSAIVSWRGSKSYGSLIQAFDREIKNESILDYYGPKQINNIDGYQKVGEGYLNKLFNVGTRVITSDEIGDLGSGSSGSMIIDSNFNLVGIHFASLNSRAYGAPNDSMIGNLFVAQSQDLSGDIDVRAAVIKKLKAQNIYTYKLNPKVSS", "aa_hexdigest": "4b676479a210856c0965744190f73727", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01732.22", "name": "Mycop_pep_DUF31", "length": 128, "aa_cov": 0.6287425149700598, "hmm_cov": 0.9609375, "evalue": 3.005934362979611e-08, "score": 29.22376251220703, "start": 7, "stop": 111 } ], "seq_stats": { "molecular_weight": 18095.066499999994, "isoelectric_point": 6.560663414001465 }, "id": "NKIECMJPJD_256", "locus": "GCA000828735_00220" }, { "type": "cds", "sequence": "contig_1", "start": 140693, "stop": 140926, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATCACGCTGGTTTAATAAACAAGAATATATAAATTATTTAGAAAATAACGCTGGTTTAATATCCTCAAACATTTATGAAGATAAAGACCGTTATCAATATTTACTATGGGGGACTGCGCCTCTAAAATCTCCAGTAAGTAATAAATATAATTCATTAAGTGGTATTAGTTTTTCAAATTTAGCAAAGGTTTATAATATTACAAATCAAAATTTCATTAATCGAGCATGA", "aa": "MKSRWFNKQEYINYLENNAGLISSNIYEDKDRYQYLLWGTAPLKSPVSNKYNSLSGISFSNLAKVYNITNQNFINRA", "aa_hexdigest": "77c8eb31efb4fe845eaa8de39dc8c46f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9019.001599999994, "isoelectric_point": 9.476095008850098 }, "id": "NKIECMJPJD_257", "locus": "GCA000828735_00221" }, { "type": "cds", "sequence": "contig_1", "start": 141041, "stop": 141664, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCGTGCAAAAATTTATTTTAATCAAAATGAAGCTAATGTTGATGATTCTAAACTTTATATGAACAAAATCGGTTATTCTGAATTTGGTAGTGATGTCCAAAAAAGATTAGAAAACGCCTTTAAAATTCGTTATGATGAGCAAAATATTTATCAAATTAATTCTCCGCAAATTTTAGCTAAAGAAACAAAAACGCTTACATCTTATATTGATAAAAAAACAAACAACAATTATAGTATTAATATAGAATTAATCAAAAAATTAATCCAAATTAATCCTTTTGGTAAACTCCCCACAAATTTCGCTCAACTAATTAATTTAATTAAAAAAGAAGAATATCCTAAATTCTTGACAATTACTAAAAACGAATCTGTTGACAATATTGTTGTTAAAGATATTTATTATCGGATTATTGATCGTTATGCAAAATTAGAATTTATTTTAGAAATTTATAACAAAAAAACAAAACAAACCGTATATTTAAGTGCTAATTTTAATCAAAAGAATTCAGGATTATTAAAAAATGAAGATTATTTTCAATATATTTTTGATCGTACCATCTCACTTGATTTACTAACAACAAAAGATGGAAAAAATGTTGAATTAAATTCTGGAACTGGTTGA", "aa": "MRAKIYFNQNEANVDDSKLYMNKIGYSEFGSDVQKRLENAFKIRYDEQNIYQINSPQILAKETKTLTSYIDKKTNNNYSINIELIKKLIQINPFGKLPTNFAQLINLIKKEEYPKFLTITKNESVDNIVVKDIYYRIIDRYAKLEFILEIYNKKTKQTVYLSANFNQKNSGLLKNEDYFQYIFDRTISLDLLTTKDGKNVELNSGTG", "aa_hexdigest": "6c804d216660383fa28f34db037428ba", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 24310.450000000015, "isoelectric_point": 9.154784584045409 }, "id": "NKIECMJPJD_258", "locus": "GCA000828735_00222" }, { "type": "cds", "sequence": "contig_1", "start": 141893, "stop": 142198, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATCTAAAAACAAAATTTTTTTTAAAAGTTATAAGTGTTATTGCTCCAATTGTTATAATTCCAACTATTTTAGCTAATTGTGCACATATAAATTCTAATGAACTTGACAATGCTAAAACAAACTTGAAAGGAAATGTTGAAGTTGTTAATATTAATCCTTATAAAACAAAACAAATGTTAGCATCAAATATCAACAAAGAACAAATTAATTCTTATTTTATTTTTATTTTTAATTTAATTAAAACAAATCAAAAAATTGAAGAAAAAATTTTAGATAAAAATGATTATGAAATTCTTAATTGA", "aa": "MNLKTKFFLKVISVIAPIVIIPTILANCAHINSNELDNAKTNLKGNVEVVNINPYKTKQMLASNINKEQINSYFIFIFNLIKTNQKIEEKILDKNDYEILN", "aa_hexdigest": "dc3a1950a4e5412c9ea66a2330850034", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11595.462199999989, "isoelectric_point": 9.041320228576659 }, "id": "NKIECMJPJD_259", "locus": "GCA000828735_00223" }, { "type": "cds", "sequence": "contig_1", "start": 142308, "stop": 144428, "strand": "+", "frame": 3, "gene": "tex", "product": "S1 motif domain-containing protein", "db_xrefs": [ "COG:COG2183", "COG:K", "KEGG:K06959", "RefSeq:WP_045470338.1", "SO:0001217", "UniParc:UPI000456592F", "UniRef:UniRef100_UPI000456592F", "UniRef:UniRef50_Q9PRB6", "UniRef:UniRef90_Q9PRB6" ], "nt": "ATGATTGATTTAATTATTAAAACACTAGTTCAAAAATTAAAAATTGAGGCAAAATATATAACAAATGTTTTGAATTTATTAGCTGATAATAATACTATTGCATTCATTGCTAGATATCGTAAACATTTGACTAACAATATGGATGAATTTCAAATTCAAGCAATTGCACATGAATATGAATATCTTACAAAATTAAATAAACGTAAAGAAGTAATTATTAAAAATTTAGAACAAAAAGGTTTATTAACTAATGACTTATTAGAATTAATTAATAATTGTCAACGGCTTGTTGATCTTGAAAATCTTTATGAACCTTATGCTTCAAATAAAAAAACAAAAGCATCAATCGCCATTGAAAAAGGATTAGAACCATTAGCATTATTAATATTAAAAAATAATCCTAATTCTAATATTAAAGAAGTTGCAAAACAATATTTAAATGAACAAGTTTTAAGTGTTGATGAAGCTATTGCTGGAGCTTGTGATATTATTGCCCAAAAAGTTGCTAACGATACAACTTTAAGAGAAATGCTTTATGAAACAATTAACAAACATGCTAAATTAATCACAAGAATTAATAAAATAACAAATGACCCAACTAAAAACTTTGCTTTATATTATGAGTTTAGTTGCCCAATTAAATATTTAAAAGCATATCAATTAATGGCTATTGATCGAGCTAATGAATTAAAAATCATTGCATTTAAACTTGATTTTAAAAAAGAATTCCTTATTGATTTTGCTATCAATAAATATACTAGAAAAATCAAAAGTAACAGCTATGACTACATTAAATCATCTGTTAATGATGGTTTTGATCGTTTATTAATTCCTTCAGTTTCAAATGCTGTTTACAAAGAAAAATTAGAGGAAGCTCACCAACAATCAGCACAAATTTTTAGTAATAATTTACAACAATTATTATTACAAAAACCTTTAAAAGGCCATGTGGTTTTAGGATTTGATCCAGGATATGTGCATGGTTGCAAATTAGCAATTGTTAATAAAAACAATCAATTATTACATACTGATATTATTTACCCTCACAAACCACAAATATTAATTACACAAGCTAAAAATACGTTAATATCCTTAATTAATAAATACGAAGTTAATACAATAGTAATTGGCAATGGAACAGCATCAAATGAAAGTGTAATTTTTATTAGTGATTTAATTAAGGAATTCAAATTAAATCTTAATTATTGTGTAATTAGTGAAGATGGTGCTTCAATTTATTCAGCATCATCAATTGCAAGTGAAGAATTTCCTAACTTAAGTGTTGAAAAACGTTCGGCAATTAGTATTGCACGAAGAATCATTGATCCTTTAAGTGAATTAATAAAAATCGATCCAAAAAGTATTGGCGTAGGTCAATATCAACACGATATTAATAAAAACATTCTTCAACAAAAAGTTGATTTTTGTATTGATTATTGTGTTAATCAAGTTGGTGTTGATGTTAATACTGCTTCAATTCCTTTGTTATCTAAAGTATCAGGTTTAAATAAGCGAAGTGCAAAAAAAATTTTTGAATATGTTAAAAAACATCAATTTATTGAAAATCGTGAACAATTAAAAACCATTCCTTACATTACCGATAAAGTTTTTGAGCAAGCCGCTGGTTTTTTACGAATTAATAATGCTAATAATTTTTTAGATAGAACAAGTATTCATCCAGAAGCATATCCAGTTGTTAATGAATTATGTAAATATTTACAATTACCAATTAATAAATTAATAAATAACTATCAAGTTTTAAATCAATTAAATCCTAATGAATTAACTACTATTCTAAAAACAGATATTTATACTATTGAAAACATTATTAATAACTTAAAAAACCCTTTACAAGATGTTCGTGATGATTATGATATTCCTATTTTACGTTCACAAATGGTTGATATTAATAATTTGAAAATAGGGATGTTAGTACAAGGAACAATTCGTAACCAAACAGAGTTTGGTAGTTTTATTGATATTGGTTTAAAAAATGATGCATTGTTACATATTTCAAACTATTGTGAAGAAGATAATTTGCATGTTAATAAAAATATTAATGTTTATATTGATAAAATAGATACAATTACTCAAAAAATTTCATTAAAGTTAAAATTATAG", "aa": "MIDLIIKTLVQKLKIEAKYITNVLNLLADNNTIAFIARYRKHLTNNMDEFQIQAIAHEYEYLTKLNKRKEVIIKNLEQKGLLTNDLLELINNCQRLVDLENLYEPYASNKKTKASIAIEKGLEPLALLILKNNPNSNIKEVAKQYLNEQVLSVDEAIAGACDIIAQKVANDTTLREMLYETINKHAKLITRINKITNDPTKNFALYYEFSCPIKYLKAYQLMAIDRANELKIIAFKLDFKKEFLIDFAINKYTRKIKSNSYDYIKSSVNDGFDRLLIPSVSNAVYKEKLEEAHQQSAQIFSNNLQQLLLQKPLKGHVVLGFDPGYVHGCKLAIVNKNNQLLHTDIIYPHKPQILITQAKNTLISLINKYEVNTIVIGNGTASNESVIFISDLIKEFKLNLNYCVISEDGASIYSASSIASEEFPNLSVEKRSAISIARRIIDPLSELIKIDPKSIGVGQYQHDINKNILQQKVDFCIDYCVNQVGVDVNTASIPLLSKVSGLNKRSAKKIFEYVKKHQFIENREQLKTIPYITDKVFEQAAGFLRINNANNFLDRTSIHPEAYPVVNELCKYLQLPINKLINNYQVLNQLNPNELTTILKTDIYTIENIINNLKNPLQDVRDDYDIPILRSQMVDINNLKIGMLVQGTIRNQTEFGSFIDIGLKNDALLHISNYCEEDNLHVNKNINVYIDKIDTITQKISLKLKL", "aa_hexdigest": "9f127fe822e52a82b367bdfe6717213b", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI000456592F", "ncbi_nrp_id": "WP_045470338.1", "uniref100_id": "UniRef100_UPI000456592F", "db_xrefs": [ "SO:0001217", "UniParc:UPI000456592F", "RefSeq:WP_045470338.1", "UniRef:UniRef100_UPI000456592F" ] }, "ips": { "uniref100_id": "UniRef100_UPI000456592F", "uniref90_id": "UniRef90_Q9PRB6" }, "psc": { "uniref90_id": "UniRef90_Q9PRB6", "gene": "Tex", "product": "S1 motif domain-containing protein", "uniref50_id": "UniRef50_Q9PRB6", "cog_id": "COG2183", "cog_category": "K", "kegg_orthology_id": "K06959" }, "pscc": { "uniref50_id": "UniRef50_Q9PRB6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PRB6" ], "product": "S1 motif domain-containing protein" }, "genes": [ "tex" ], "id": "NKIECMJPJD_260", "locus": "GCA000828735_00224" }, { "type": "cds", "sequence": "contig_1", "start": 144434, "stop": 145180, "strand": "+", "frame": 2, "gene": null, "product": "Iron ABC transporter substrate-binding protein", "db_xrefs": [ "RefSeq:WP_006688534.1", "SO:0001217", "UniParc:UPI00000C1B71", "UniRef:UniRef100_A0AAC9T360", "UniRef:UniRef50_A0AAC9T360", "UniRef:UniRef90_A0AAC9T360" ], "nt": "ATGAAAATAAAATTAAAAAAAATAATCATGTCTTTTTTGGGATTGCCAATTTTGGCAATTCCCCTTATCGTTAGCGCTTGTTCGCAATTTAAAATTAACCAGGATGTTATTTTAACTTATCGTCCAACAGTTTCATTAGCAAAAGAATTTGCTAATGATAACAATACTATTGATGATGTTTTAAAAAAAGTAAAAATCAATAAAGACGGATCAAATAACCTACTAGTTTATGATTTATCAAGAAATCCAAAGAATGTCCAGTATAAAATTGTTGATATTAAAAAGGATTCAGAACAAATTCTTAAAGTTACGATTAATGCTAAAATTCAAAAATATAAAGAATCAATTAATTATGATTTTTATTTTCAACCTTTTTTAACTCCTAAACAAAAAGAAGATAAAACAATCCTTCAACAAAATTTAAATATTATTAAAAGTGCTTGGGATTATGATCAAAAACAAAAAATAGGTTTTTTTAATCTTAGAATAAAACCAGAATATCAAAAAAGAAGTTTGGTTGAAATTAAAACATTAGGTGAAAAAGCTTTTAATTTTGGTGAGGATTTAATTCCTCAATTAAAAGTAGACTCAAAATTTAAAGATATTAATATTAAAATAATAGACATTAACTATTTTGAACCATTAGATGAAAGACAACGTGAATTAGTTGTGGAAATAATGATTAGTAAAGGTAAAAATGAACAACAGGCTCAATTATTTAAAAAAATTAAAATTAATTTAGACTAA", "aa": "MKIKLKKIIMSFLGLPILAIPLIVSACSQFKINQDVILTYRPTVSLAKEFANDNNTIDDVLKKVKINKDGSNNLLVYDLSRNPKNVQYKIVDIKKDSEQILKVTINAKIQKYKESINYDFYFQPFLTPKQKEDKTILQQNLNIIKSAWDYDQKQKIGFFNLRIKPEYQKRSLVEIKTLGEKAFNFGEDLIPQLKVDSKFKDINIKIIDINYFEPLDERQRELVVEIMISKGKNEQQAQLFKKIKINLD", "aa_hexdigest": "684f7ff4d7e539a9270917291964e359", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00000C1B71", "ncbi_nrp_id": "WP_006688534.1", "uniref100_id": "UniRef100_A0AAC9T360", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1B71", "RefSeq:WP_006688534.1", "UniRef:UniRef100_A0AAC9T360" ] }, "ips": { "uniref100_id": "UniRef100_A0AAC9T360", "uniref90_id": "UniRef90_A0AAC9T360" }, "psc": { "uniref90_id": "UniRef90_A0AAC9T360", "product": "Iron ABC transporter substrate-binding protein", "uniref50_id": "UniRef50_A0AAC9T360" }, "pscc": { "uniref50_id": "UniRef50_A0AAC9T360", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T360" ], "product": "Iron ABC transporter substrate-binding protein" }, "genes": [], "id": "NKIECMJPJD_261", "locus": "GCA000828735_00225" }, { "type": "cds", "sequence": "contig_1", "start": 145195, "stop": 145686, "strand": "+", "frame": 1, "gene": null, "product": "Iron ABC transporter substrate-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B5ZAK0", "UniRef:UniRef90_A0AAC9T040" ], "nt": "GTGAATAAAAAAATTAAAACAGTTTTCATTAGTAGTTTAATTGTTGGACTATTAGGTTTGATAACAACAAGTGGTGTTGCGTGCAGCTCATCAATAAAATCAAAACAAGAGAATGAAATTTATCCAACCATTAAAGGTTATGATCAACATGATTTTTCAAAGATTTTAGCTTCAGAATTTATTAAGATTATTAAAACTAATATTCAATTTTTGGCACCAAATAAAGATATTAAATATGAATTATATGATGCACGATTGAATGAGTTAGATGAAGATGTAATTGATCTAATAATTCGTTATCATTTTAAAGGTCAAACGCACACTTACAAAAAAATCTTAAAAGGTTTTAAGAATGCTAAACAATATCGTTTATTTTTAGAAAATAAAAAATATTTAGAAGATGAAAAAAACCATAATACTCTTTATTTAATGCCCATAGTTAATTCAAGTATTAACATTAGTAAAACAACAGTAGATGAAGCATTAAAATGA", "aa": "MNKKIKTVFISSLIVGLLGLITTSGVACSSSIKSKQENEIYPTIKGYDQHDFSKILASEFIKIIKTNIQFLAPNKDIKYELYDARLNELDEDVIDLIIRYHFKGQTHTYKKILKGFKNAKQYRLFLENKKYLEDEKNHNTLYLMPIVNSSINISKTTVDEALK", "aa_hexdigest": "bd94d71da206b4e2e5872e2317c2995f", "start_type": "GTG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 145195, "stop": 145989, "observations": { "mutation": [ 145684 ], "stop": [ 145684 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAC9T040", "query_cov": 1.0, "subject_cov": 0.5046439628482973, "identity": 1.0, "score": 317.0, "evalue": 3.1e-107, "gene_start": 1, "gene_end": 163, "reference_start": 1, "reference_end": 163, "reference_sequence": "MNKKIKTVFISSLIVGLLGLITTSGVACSSSIKSKQENEIYPTIKGYDQHDFSKILASEFIKIIKTNIQFLAPNKDIKYELYDARLNELDEDVIDLIIRYHFKGQTHTYKKILKGFKNAKQYRLFLENKKYLEDEKNHNTLYLMPIVNSSINISKTTVDEALKWQNSYLNINNSNNDLKYEILKITKSKTANSIDIEIKISKGNGENLASITYNKTLNGFISEADKKIYLANLEAIKQDKNQLEPQLIDEKTKQKTIKEVIENYKTLFKLPFQTGFIYEIQSVEPDVNNKTAVVFKILIKKGNGSQEAVLEYLKTIKGFNQNK" }, "paralog": false, "identity": 0.9924242424242424, "score": 512.0, "evalue": 2.34e-186, "description": "Internal stop codon at 145684. Nonsense mutation around 145684.", "psc": { "uniref90_id": "UniRef90_A0AAC9T040", "product": "Iron ABC transporter substrate-binding protein", "uniref50_id": "UniRef50_B5ZAK0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAC9T040", "UniRef:UniRef50_B5ZAK0" ] }, "pscc": { "uniref50_id": "UniRef50_B5ZAK0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B5ZAK0" ], "product": "Putative ABC substrate-binding protein-iron" } }, "id": "NKIECMJPJD_262", "locus": "GCA000828735_00226" }, { "type": "cds", "sequence": "contig_1", "start": 146266, "stop": 146487, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCTCAAATGGAAAATTCGACGAAAAAAGTTGAATCGGTAGCTAACGTTGAAGTAGTTACTACTGAAAATGCAAAAGTAGAACAAAAACCTACATCACCGGCTGATGCAATTCAGCTTAAAGTCAATGCAAATTCAACTATAAAAAATCTTAAAACTTTAGTTTCAATTGTAAAATTAACTGAATCAGGTGCTCATATTGGGTTAAATCCTAAGAAATGA", "aa": "MSQMENSTKKVESVANVEVVTTENAKVEQKPTSPADAIQLKVNANSTIKNLKTLVSIVKLTESGAHIGLNPKK", "aa_hexdigest": "3e196d22ba640387d34dd4dc42cc75fc", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7831.9281999999985, "isoelectric_point": 9.39911975860596 }, "id": "NKIECMJPJD_263", "locus": "GCA000828735_00227" }, { "type": "cds", "sequence": "contig_1", "start": 146497, "stop": 146709, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTAGTTACATCCATGCTAAACGTTCAAATAACCATGTAATTGATATTTTAAAAACAATTCTATTTTTAGATCGTGCATACAAATTCTTACAAGAGGTTGCTCAAAACGGGGGCACTGTGATGTTTGTGGGTACAAGAGGTCGTGTTGTTAAAGAATTAATTAAAGCAGAAGCAGAACGTACTAATTCATTTTATGTAACTCAAAGATGA", "aa": "MASYIHAKRSNNHVIDILKTILFLDRAYKFLQEVAQNGGTVMFVGTRGRVVKELIKAEAERTNSFYVTQR", "aa_hexdigest": "e2f614f0e417e06b925df69340e0702f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8045.222199999999, "isoelectric_point": 9.9877161026001 }, "id": "NKIECMJPJD_264", "locus": "GCA000828735_00228" }, { "type": "cds", "sequence": "contig_1", "start": 146881, "stop": 147165, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAACAACGTCCAGATGTTTTAATTCTAGTAGATCCTGTTAATGATGTTAATGCTATTAAAGAAGCACGTAAATTAAACATTCCAGTAATTGCTTTAGCAAATACGAATGCTGATCCACAATTGATTGATTACATTATTCCAGTAAATAATTATTCAGTTAAATCAATTACTTTGATTTTAGGTGTGCTAGCAGATTCAATTGCTGAATTGCACGGTGAACCAACAAAAATTGTTGGACGACCAGATAGCGAAATTGTTTTACCTGAAACAAAATCAAATTGA", "aa": "MKQRPDVLILVDPVNDVNAIKEARKLNIPVIALANTNADPQLIDYIIPVNNYSVKSITLILGVLADSIAELHGEPTKIVGRPDSEIVLPETKSN", "aa_hexdigest": "9b4b263f85f3487695270e9222c0826d", "start_type": "ATG", "rbs_motif": "GGxGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10236.748999999993, "isoelectric_point": 4.932169532775878 }, "id": "NKIECMJPJD_265", "locus": "GCA000828735_00229" }, { "type": "cds", "sequence": "contig_1", "start": 147482, "stop": 147607, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAAATTATACCTTTCGTTTTAAACTATTTAAACAAATTAAAAATCAACAAGTTAAAAAATTAAAGCCACTCATTTTAATAGGAGCAACAGTTTTTTTAATTATTGCTATTTTTAGTATTTGA", "aa": "MQNYTFRFKLFKQIKNQQVKKLKPLILIGATVFLIIAIFSI", "aa_hexdigest": "8673c47d07bd900127bc08554dda3993", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4855.955599999998, "isoelectric_point": 10.751730918884277 }, "id": "NKIECMJPJD_266", "locus": "GCA000828735_00230" }, { "type": "cds", "sequence": "contig_1", "start": 147809, "stop": 148177, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGACACAAGCACTAACTAAAAATCCATTAGCTGATTCAAGTACATTAGGTATTGTACAATCAAGTGTATTTATGATTGTAATTGCATTCTCATATAACATGATTTCTTTTGGAGAACTTTTTGGTTTTGCCATTATTGGTGGAATAATTGCTAGCATTATTTTATTGATTCTAGTTTTTGCAACAAGAAGCAGATTATCATATATTAAAATTACTTTAGCAGGATTAGCTATAGGAGTTTTTTTTAATACAATTTCTTATTTTATTCGTATTCAAAGTGCTAAAGGTTCAAGTATTAATTTTAAATATGTTTTAGGGGGTAGTGAAAATATTTATACAACTCATATTGATCCTTTTTTAACATTATGA", "aa": "MTQALTKNPLADSSTLGIVQSSVFMIVIAFSYNMISFGELFGFAIIGGIIASIILLILVFATRSRLSYIKITLAGLAIGVFFNTISYFIRIQSAKGSSINFKYVLGGSENIYTTHIDPFLTL", "aa_hexdigest": "600e1741effd74e090a15876ba41e3f9", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13270.518699999975, "isoelectric_point": 9.45462703704834 }, "id": "NKIECMJPJD_267", "locus": "GCA000828735_00231" }, { "type": "cds", "sequence": "contig_1", "start": 148394, "stop": 148522, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCGTAAAATTTTTTCAATTAGAGATTATCGTTATGTTATGCCCTTATCAGGATTATTAGGTGCAATGATAACATCTTTAGGTTTAATACTATATCGTGAGACAATTTATATTAATTCATCGATTTGA", "aa": "MRKIFSIRDYRYVMPLSGLLGAMITSLGLILYRETIYINSSI", "aa_hexdigest": "04c56e621443a3607de131d51d150698", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4869.810099999999, "isoelectric_point": 9.815069770812986 }, "id": "NKIECMJPJD_268", "locus": "GCA000828735_00232" }, { "type": "cds", "sequence": "contig_1", "start": 148609, "stop": 148743, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTTTAATAATGAAAATTAGTTTTAAATTTAGATTAATTAATGTAATTTCAATGCTTGGATTAAGTATTATTTTTACAACATTAAGTTCATGTAAAAATCTTCAACAACAAAAACCGTATGATACTAATTGA", "aa": "MVLIMKISFKFRLINVISMLGLSIIFTTLSSCKNLQQQKPYDTN", "aa_hexdigest": "f4b9af083612899ba6ff3732aee7b71b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5079.116400000003, "isoelectric_point": 9.864903831481932 }, "id": "NKIECMJPJD_269", "locus": "GCA000828735_00233" }, { "type": "cds", "sequence": "contig_1", "start": 148768, "stop": 148902, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATAATTTTTTTCGTGATCCTTATAAAATTCATTTAGAATTTGCTCGCCAATTAGATAATGTTTTAAATTTTAATGAATTAAAAAAACGTCTACCAAAACCAATGCAAAATGTACATTCATTTACTGAATGA", "aa": "MNNFFRDPYKIHLEFARQLDNVLNFNELKKRLPKPMQNVHSFTE", "aa_hexdigest": "321dfe0f6068a8cbd6ba7632850a5bf9", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5421.177500000001, "isoelectric_point": 9.396541023254393 }, "id": "NKIECMJPJD_270", "locus": "GCA000828735_00234" }, { "type": "cds", "sequence": "contig_1", "start": 148972, "stop": 149433, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCGCAATCATAAAGCAACTGTTTTAGTTATAGAAAATCGTAATGAATTAGTTAATAATAAAGAAAATTATAATAAATTTTCTATTCTAACACCTTCAGATTTTCCACTAATCTATTCTAATCCTTATACAGAAGTTCCAGGATTGGGATTGAATTTTCCAAAACCAATTAATAATGATATTAGTGATATTATTGATCGTTATCAATCAATTGGTTATAATGCATTAAGAACAAACCCGCTCGAATCAGTCTTAAAACAAACATCTTCATTTTATAAAACAGCTGATTATGTTTTTTATATGTATGATTCTAATTTATTTACTAATAATAAATACAAAACAGATGGTCAAAAACGTGAAGATTTATTTAAAGAAATTTTATCAAACAAAAATTTTTATGCTCATAAATTCTTACGCAATTCTCGTTCAAAAATTATTTTTATTGATCGTTCATTGTTTTGA", "aa": "MRNHKATVLVIENRNELVNNKENYNKFSILTPSDFPLIYSNPYTEVPGLGLNFPKPINNDISDIIDRYQSIGYNALRTNPLESVLKQTSSFYKTADYVFYMYDSNLFTNNKYKTDGQKREDLFKEILSNKNFYAHKFLRNSRSKIIFIDRSLF", "aa_hexdigest": "2ae4db60647d84a2da83956365a0a467", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18046.192900000002, "isoelectric_point": 9.299773979187012 }, "id": "NKIECMJPJD_271", "locus": "GCA000828735_00235" }, { "type": "cds", "sequence": "contig_1", "start": 149785, "stop": 149940, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTAATTATGATAAATTAGAAAAAATTAATAAGGCTCCTAATGATAGTGAAAAAGCGGAATTTGTTGATAAAAATTTTGCAATTTATATGGGCCCATCCCATTTATTAAACTCAATGAATAAATTAATTATGATTAAAAATACAGGATCTTGA", "aa": "MINYDKLEKINKAPNDSEKAEFVDKNFAIYMGPSHLLNSMNKLIMIKNTGS", "aa_hexdigest": "d78386c7d6063e86cb10e86368b4df75", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5817.690299999999, "isoelectric_point": 8.029811286926268 }, "id": "NKIECMJPJD_272", "locus": "GCA000828735_00236" }, { "type": "cds", "sequence": "contig_1", "start": 150227, "stop": 150769, "strand": "+", "frame": 2, "gene": null, "product": "Ferrichrome ABC transporter", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PRC2", "UniRef:UniRef90_Q9PRC2" ], "nt": "TTGTTTTTTTATTCTCCAATTGTTTTATTAGTTTGTGGTGCAGGATTGGTTTGCAGTGGATTTAGTTTGCAATCTACAACGCGTAATGGTTTGGCAGGTCCATCAACATTAGGAATTTTTCCATTAGCAGCCTTAGGTAATATTTTGAGTCAGTTAATTACCAAAAGTTTTTCTAATATTTTTATTGGCTATGTTTTTGGAATTATTTTTAGCTTATTTGCTTTAGGAATCAATTTTTTATTTATTAGAATTTCACCACATAAGAAAACTTTTAAACCTATTATTTTTGGTTTTTCATTAGGTGCCATTATCACAGCTGTTAATATTTTAATTGCTAATTTTAATTCATCAATTGTCGCTTCCCCCATTCAATTATTAGGTAGTATTGGTGTTTACAATTCTATTGAGCGCTTTTATATTGGCACTCCAATAGTGTTTATAAGTACGATTATTTTGTTATGTTATGCTAAAAAAATCCAAATTTTAGATACGGATCATTATTTAGCTAAATCACTAGGAATCGATGTTAATAAAGTGTTTTGA", "aa": "MFFYSPIVLLVCGAGLVCSGFSLQSTTRNGLAGPSTLGIFPLAALGNILSQLITKSFSNIFIGYVFGIIFSLFALGINFLFIRISPHKKTFKPIIFGFSLGAIITAVNILIANFNSSIVASPIQLLGSIGVYNSIERFYIGTPIVFISTIILLCYAKKIQILDTDHYLAKSLGIDVNKVF", "aa_hexdigest": "351dd1df52d014bd5ac4049adff32a11", "start_type": "TTG", "rbs_motif": "GGAG/GAGG", "genes": [], "truncated": "both", "pseudogene": { "start": 150038, "stop": 151063, "observations": { "mutation": [ 150098, 150179, 150767, 151031 ], "stop": [ 150098, 150179, 150767, 151031 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PRC2", "query_cov": 1.0, "subject_cov": 0.5278592375366569, "identity": 0.9940000000000001, "score": 341.0, "evalue": 4.27e-116, "gene_start": 1, "gene_end": 180, "reference_start": 64, "reference_end": 243, "reference_sequence": "MKIKLKFENKLNFKKYLIYKWSVFVVLVLLTSFIFIFNIIYTNKTFIWESFYFQNASFEEVFLLFFYSPIVLLVCGAGLVCSGFSLQSTTRNGLAGPSTLGIFPLAALGNILSQLITKSFSNIFIGYVFGIIFSLFALGINFLFIRISPHKKTFKPIIFGFSLGAIITAVNILIANFNSSIVASPIQLLGSIGVYNSIERFYIGTPIVFISTIILLCYAKKIQILDTDHYLAKSLGIDVNKVFWITSTCSIFIAISTTFLIGGLSLIAIVMPHLVRLLFKRTNYLFQMIIAIFLTTFLLELLGIIVEFFSKFNIVFLVATVFAIPMIVLLKWELGWKVNKN" }, "paralog": false, "identity": 0.9882697947214076, "score": 642.0, "evalue": 2.1e-237, "description": "Internal stop codon at 150098, 150179, 150767, 151031. Nonsense mutation around 150098, 150179, 150767, 151031.", "psc": { "uniref90_id": "UniRef90_Q9PRC2", "product": "Ferrichrome ABC transporter", "uniref50_id": "UniRef50_Q9PRC2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PRC2", "UniRef:UniRef50_Q9PRC2" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PRC2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PRC2" ], "product": "Ferrichrome ABC transporter" } }, "id": "NKIECMJPJD_273", "locus": "GCA000828735_00237" }, { "type": "cds", "sequence": "contig_1", "start": 150848, "stop": 151033, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCCCATTTAGTTCGTTTACTTTTTAAAAGAACTAATTATTTATTTCAAATGATTATCGCCATTTTTCTAACTACTTTTTTATTAGAATTACTTGGAATAATTGTAGAATTTTTTTCAAAATTTAATATTGTTTTTTTAGTAGCAACAGTTTTTGCGATTCCAATGATTGTTTTGCTAAAATGA", "aa": "MPHLVRLLFKRTNYLFQMIIAIFLTTFLLELLGIIVEFFSKFNIVFLVATVFAIPMIVLLK", "aa_hexdigest": "47f6a8c1a55f84ed434469ee70f5ee6f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7161.876999999997, "isoelectric_point": 9.992035484313966 }, "id": "NKIECMJPJD_274", "locus": "GCA000828735_00238" }, { "type": "cds", "sequence": "contig_1", "start": 151064, "stop": 151249, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTATAAAAATTAAGCATGATCAAAATTTAAAACACCAATTTGAACAAAAATTATTTTTTCGTTTTAAAAAGAACAAATTTTTTCTTTCATTAAGTTTATTAATAATAACAATTATTTTAACGCTGATTGCTGTAGCTGGAGCCTCGCGTTTTATAAATGTTTTTAGTTATTTGAGCGATTGA", "aa": "MSIKIKHDQNLKHQFEQKLFFRFKKNKFFLSLSLLIITIILTLIAVAGASRFINVFSYLSD", "aa_hexdigest": "7794f2eca4d83913b3a1ffd7babad4fd", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7176.512999999997, "isoelectric_point": 10.214580345153806 }, "id": "NKIECMJPJD_275", "locus": "GCA000828735_00239" }, { "type": "cds", "sequence": "contig_1", "start": 151268, "stop": 152086, "strand": "+", "frame": 2, "gene": "fepD", "product": "Ferrichrome ABC transporter", "db_xrefs": [ "COG:COG0609", "COG:P", "SO:0001217", "UniRef:UniRef50_Q9PRC3", "UniRef:UniRef90_Q9PRC3" ], "nt": "TTGTTTTCTGGAATTGCATTAGGAATTAGTGGTTATATTCTTCAAAGATTAACAAAAAATCGTTTAGCAGATAGTTCTTTATTAGGAATGGGCAATATTAACTTAGTGATTTTAACAATACTTTTTTTAATATTTGATTTTGGGCAATTGCAAATCCAAAAACGAATGGAATATGTTCTACCATTTATTTATTTAATTGGGTCAACAATGATTTGTTTTTTTATTCATTTTTTATGCAATTCTTCTACTGGATACATTTTTAAAAGAATTATTGTTTCAGGTATTATTATTAATTTGATTACAATTGTTATTGCTCAGTCATTAAGAATTTTAATGAGTAAAGAATCAAGTATGTATTTAAAAACAATTTTATTAGGTAATATTGAATCACGCAGCGATTTTTGTTTTTATTTTTGTTTTGGAATGCTTACTATTAGTGTTATTTGGTTAATGTTAAATGCAACTAAATTTAAGATTATGGTAACAAATCAACAAATATCTGGACAGTTAGGCATTAATAATAAGTCCTTGACAATTCAAACTTTTATTTGCATTAGCTTATTAGTATCGGTAAGTTATAGTTTAAGTGGTAATGTTATTTTTGTTGGGATTGTTGCAGCTAATTTTTCTTTCAATTATGTAAAAAACAATATTTCTAATGGCATTATTAATGCTGGATTAATAGGCGGCATTGTATTATTGATTAGTTATATTTTTGTTGTTTTAATTTTAAATTTATCAACTGACCAGACTATTTTATTAGTGCCAATTATTTCAGGACCATATTTTTTATATCAAGTTATTTTATTGAAAAATTAA", "aa": "MFSGIALGISGYILQRLTKNRLADSSLLGMGNINLVILTILFLIFDFGQLQIQKRMEYVLPFIYLIGSTMICFFIHFLCNSSTGYIFKRIIVSGIIINLITIVIAQSLRILMSKESSMYLKTILLGNIESRSDFCFYFCFGMLTISVIWLMLNATKFKIMVTNQQISGQLGINNKSLTIQTFICISLLVSVSYSLSGNVIFVGIVAANFSFNYVKNNISNGIINAGLIGGIVLLISYIFVVLILNLSTDQTILLVPIISGPYFLYQVILLKN", "aa_hexdigest": "fd620f9f840d60df035b5eb3d3ac9b28", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_Q9PRC3", "query_cov": 1.0, "subject_cov": 0.8, "identity": 0.996, "score": 494.0, "evalue": 6.62e-175, "valid": true, "gene": "FepD", "product": "Ferrichrome ABC transporter", "uniref50_id": "UniRef50_Q9PRC3", "cog_id": "COG0609", "cog_category": "P" }, "pscc": { "uniref50_id": "UniRef50_Q9PRC3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PRC3" ], "product": "Ferrichrome ABC transporter" }, "genes": [ "fepD" ], "id": "NKIECMJPJD_276", "locus": "GCA000828735_00240" }, { "type": "cds", "sequence": "contig_1", "start": 152186, "stop": 152434, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTATGGAATTAATTAAGACTAACACACTTAATTTTTATTTTGAGCCATCTAAAAGCGAACTAAAAAAAGGTTCGATTGTTTTTATACACGGCCTTGATGCATCTCCACATTATTTTTTTTTAATTAATAAAGATTTATCTGATTATGATTGTTATTTTGTTGGATTACCTGCACATGGTTTAACACCTATTAATAACAAAAAAGATTTAAATATTAAAGTTTTTGCTGAGTTATTTATTAATTGA", "aa": "MIMELIKTNTLNFYFEPSKSELKKGSIVFIHGLDASPHYFFLINKDLSDYDCYFVGLPAHGLTPINNKKDLNIKVFAELFIN", "aa_hexdigest": "932181009e9fb87062aa1af959f1ebed", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9443.868599999993, "isoelectric_point": 6.244752311706543 }, "id": "NKIECMJPJD_277", "locus": "GCA000828735_00241" }, { "type": "cds", "sequence": "contig_1", "start": 152465, "stop": 153055, "strand": "+", "frame": 2, "gene": "menH", "product": "Triacylglycerol lipase", "db_xrefs": [ "COG:COG0596", "COG:HR", "SO:0001217", "UniRef:UniRef50_Q9PRC4", "UniRef:UniRef90_Q9PRC4" ], "nt": "TTGTTAGGTCATTCATTAGGAGCGGGGATTGCTAGTTTGGTTAGTTTTATTATCCCCCAACGCATTGAAAAATTAATTCTTGTTTGTCCATATCATTATCAATATTTAAATCCGTTTTTAAATAAAAAATTATTTAATGCTTGGGTTTTATTTCCCAATCCATTTTTAAAATTTAAAACAGATGTCGTTTTAAAAAAATTATATATTGATTATCGTAATAATTATAAAACCTTAATAGAAACTCGTTGGGAGTCAATTTCAAGAGAATATCCACGAATTGCACGCGATGTCTTATTTTTATGTTTATCGTTATTAAATATTAAAATCAATCATGAATTAAAAATGGCACAGCGTAATTTAATAATGCCAACATTAATTATGGTTTCAAGACATGATCAATTAATTGATTTTAATTTAGCTATTAAAGTGTTTAAAAACAACAATAAAGTAAATCAATATATTTTTAATAATTCTGGTCATATTCCTTTTATTGAAGAACCAAAATTATTTACTAATATATTACTAAGTTTTTTAGAGGATAGATTTGTAGAACAAGAGGAAAATGATAACAATGATATTAACGAAAAATAG", "aa": "MLGHSLGAGIASLVSFIIPQRIEKLILVCPYHYQYLNPFLNKKLFNAWVLFPNPFLKFKTDVVLKKLYIDYRNNYKTLIETRWESISREYPRIARDVLFLCLSLLNIKINHELKMAQRNLIMPTLIMVSRHDQLIDFNLAIKVFKNNNKVNQYIFNNSGHIPFIEEPKLFTNILLSFLEDRFVEQEENDNNDINEK", "aa_hexdigest": "a39a94b4203f097355b929297f938f0d", "start_type": "TTG", "rbs_motif": null, "genes": [ "menH" ], "truncated": "5-prime", "pseudogene": { "start": 152186, "stop": 153055, "observations": { "mutation": [ 152432 ], "stop": [ 152432 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PRC4", "query_cov": 1.0, "subject_cov": 0.6782006920415224, "identity": 0.995, "score": 384.0, "evalue": 1.78e-133, "gene_start": 1, "gene_end": 196, "reference_start": 94, "reference_end": 289, "reference_sequence": "MIMELIKTNTLNFYFEPSKSELKKGSIVFIHGLDASPHYFFLINKDLSDYDCYFVGLPAHGLTPINNKKDLNIKVFAELFINWINEIDLKEFHLLGHSLGAGIASLVSFIIPQRIEKLILVCPYHYQYLNPFLNKKLFNAWVLFPNPFLKFKTDVVLKKLYIDYRNNYKTLIETRWESISREYPRIARDVLFLCLSLLNIKINHELKMAQRNLIMPTLIMVSRHDQLIDFNLAIKVFKNNNKVNQYIFNNSGHIPFIEEPKLFTNILLSFLEDRFVEQEENDNNDINEK" }, "paralog": false, "identity": 0.9965397923875432, "score": 584.0, "evalue": 5.72e-215, "description": "Internal stop codon at 152432. Nonsense mutation around 152432.", "psc": { "uniref90_id": "UniRef90_Q9PRC4", "gene": "MenH", "product": "Triacylglycerol lipase", "uniref50_id": "UniRef50_Q9PRC4", "cog_id": "COG0596", "cog_category": "HR", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PRC4", "UniRef:UniRef50_Q9PRC4", "COG:COG0596", "COG:HR" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PRC4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PRC4" ], "product": "Triacylglycerol lipase" } }, "id": "NKIECMJPJD_278", "locus": "GCA000828735_00242" }, { "type": "cds", "sequence": "contig_1", "start": 153036, "stop": 153728, "strand": "+", "frame": 3, "gene": "tmk", "product": "dTMP kinase", "db_xrefs": [ "COG:COG0125", "COG:F", "EC:2.7.4.9", "GO:0004798", "GO:0005524", "GO:0005829", "GO:0006227", "GO:0006233", "GO:0006235", "KEGG:K00943", "RefSeq:WP_006688582.1", "SO:0001217", "UniParc:UPI000012E114", "UniRef:UniRef100_Q9PRC5", "UniRef:UniRef50_Q9PRC5", "UniRef:UniRef90_Q9PRC5" ], "nt": "ATGATATTAACGAAAAATAGTAATGAAAAAAAACCGCTTAAAAAAGGATTATTTATTGTTTTTGAAGGAATTGATGGTGCAGGGAAAACTTCAATCTTAAAACAACTTTTAGAGGTTTTAAAAGAACCTAAACTAGTTAATAAAATTTTTTTAACGCGTGAACCTGGTGGAAAAAACAATAATGCAGCAGAGATGATACGTGAGTTTTTTTTAAAAAATTTAGAAGTTTTTGATCCGTTAACACTAGCTTATTTATATGCAAGCAGTCGTGCTGAACACGTAAAAAAAACAATCAATCCTCATTTAGAAAAAGATCATATTGTAATTTCAGATCGTTTTGTTCATTCTTCATATATTTATCAAGGTATTGTTCAAAATCAAAGTTTAGATGTTATTTACCAAATTAATCAACAAGCAATTGGTGAATTAGAAATAGATTATGTTTTTTATTTTGATGTTAATGTTAATAATGCATTAAATCGAATGAAAAATCGTTTTGACAATACAAATGCATTCGATTCACAAAATAAACAATTTTATGAAAAGTTATTAAAACAATACCCTAGTGTTTTTAAAGTTTATAATCAACCTAAAAAAATTATTTTTATTGATGCCAATAAAAATGAAAATGAAGTATTATGTGAGGTTAAAGAACAATTATTAAAAATTTTTAAAGAACATAAATATATATAA", "aa": "MILTKNSNEKKPLKKGLFIVFEGIDGAGKTSILKQLLEVLKEPKLVNKIFLTREPGGKNNNAAEMIREFFLKNLEVFDPLTLAYLYASSRAEHVKKTINPHLEKDHIVISDRFVHSSYIYQGIVQNQSLDVIYQINQQAIGELEIDYVFYFDVNVNNALNRMKNRFDNTNAFDSQNKQFYEKLLKQYPSVFKVYNQPKKIIFIDANKNENEVLCEVKEQLLKIFKEHKYI", "aa_hexdigest": "c0003d9cf5fd74eb39b89aaf2a4b4f23", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "ups": { "uniparc_id": "UPI000012E114", "ncbi_nrp_id": "WP_006688582.1", "uniref100_id": "UniRef100_Q9PRC5", "db_xrefs": [ "SO:0001217", "UniParc:UPI000012E114", "RefSeq:WP_006688582.1", "UniRef:UniRef100_Q9PRC5" ] }, "ips": { "uniref100_id": "UniRef100_Q9PRC5", "uniref90_id": "UniRef90_Q9PRC5" }, "psc": { "uniref90_id": "UniRef90_Q9PRC5", "gene": "tmk", "product": "dTMP kinase", "ec_ids": [ "2.7.4.9" ], "uniref50_id": "UniRef50_Q9PRC5", "cog_id": "COG0125", "cog_category": "F", "kegg_orthology_id": "K00943", "go_ids": [ "GO:0004798", "GO:0005524", "GO:0005829", "GO:0006227", "GO:0006233", "GO:0006235" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PRC5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PRC5" ], "product": "Thymidylate kinase" }, "genes": [ "tmk" ], "id": "NKIECMJPJD_279", "locus": "GCA000828735_00243" }, { "type": "cds", "sequence": "contig_1", "start": 153729, "stop": 154523, "strand": "+", "frame": 3, "gene": "dnaX", "product": "DNA polymerase III delta subunit", "db_xrefs": [ "COG:COG2812", "COG:L", "RefSeq:WP_006688710.1", "SO:0001217", "UniParc:UPI00000C1B6A", "UniRef:UniRef100_Q9PRC6", "UniRef:UniRef50_Q9PRC6", "UniRef:UniRef90_Q9PRC6" ], "nt": "ATGAAATACAGTTTTGCTAATTTATTAATTCAATCACCTAAAACCTCACTAGAATCAGGTATTGAAGAAATTATGTTAGCTTTTATTTATGAGAAAAATCATAAAGAACAATCATTTTATGTTACTAAAGTTAAAAATAAACAATACTTTGATTTAAAAATTTATGATGGACTATCAATGAAAAAAAGCGATGTAATTGATTTACAAAACACTTTTTTATGTGATGGAGTAGAAGATATTAATTTAAAATTTTATCTTATTAAAAATATTGACTTAGCATCAAAATATGTTTTAAATGCATTATTAAAATTTATTGAAGAACCGCCAAAAAATACAATTGCAATTTTTAGCACTAAAAATTTAAATCAAGTTTTAAAAACTATTAAAAGTCGTTGTCAGCTTTTTTATTTACCTACTAATCATGATTTATATAAAAAATTAATTAACCAAATTAATCAACCAATTGATCTCGTGGAATGCGACTTAATGTTTGATGATTTGGATGAGCTAAAAATTTTATTAGAAAACAAGGAAATCAATGATGTTTTAACTTATTATGGAAAATTAAGTGATTTAAGAAGTTTTGAAATATTAAATGATTTAAAAGAAATATTTAAAAATTTATCTACCTTACAAATTCATTATCTATTAAAATTAATCTTCATTAGAATTAATAATGTAAATAGCAAAGAAATTATTTTGGATTTAATGCGTGCGAATTTAAAAATTAATATTAATAAAAATAATTTGTTTACTATTATTTATACAATTATTAATAAAAATAAAGGTGATTAA", "aa": "MKYSFANLLIQSPKTSLESGIEEIMLAFIYEKNHKEQSFYVTKVKNKQYFDLKIYDGLSMKKSDVIDLQNTFLCDGVEDINLKFYLIKNIDLASKYVLNALLKFIEEPPKNTIAIFSTKNLNQVLKTIKSRCQLFYLPTNHDLYKKLINQINQPIDLVECDLMFDDLDELKILLENKEINDVLTYYGKLSDLRSFEILNDLKEIFKNLSTLQIHYLLKLIFIRINNVNSKEIILDLMRANLKININKNNLFTIIYTIINKNKGD", "aa_hexdigest": "93a0e601525ade37897dc06432aafa6f", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00000C1B6A", "ncbi_nrp_id": "WP_006688710.1", "uniref100_id": "UniRef100_Q9PRC6", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1B6A", "RefSeq:WP_006688710.1", "UniRef:UniRef100_Q9PRC6" ] }, "ips": { "uniref100_id": "UniRef100_Q9PRC6", "uniref90_id": "UniRef90_Q9PRC6" }, "psc": { "uniref90_id": "UniRef90_Q9PRC6", "gene": "DnaX", "product": "DNA polymerase III delta subunit", "uniref50_id": "UniRef50_Q9PRC6", "cog_id": "COG2812", "cog_category": "L" }, "pscc": { "uniref50_id": "UniRef50_Q9PRC6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PRC6" ], "product": "DNA polymerase III delta subunit" }, "genes": [ "dnaX" ], "id": "NKIECMJPJD_280", "locus": "GCA000828735_00244" }, { "type": "cds", "sequence": "contig_1", "start": 154526, "stop": 155842, "strand": "+", "frame": 2, "gene": "mnmE", "product": "tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE", "db_xrefs": [ "COG:COG0486", "COG:J", "EC:3.6.-.-", "GO:0002098", "GO:0003924", "GO:0005525", "GO:0005829", "GO:0006400", "GO:0030488", "GO:0046872", "RefSeq:WP_006688774.1", "SO:0001217", "UniParc:UPI0000137467", "UniRef:UniRef100_Q9PRC7", "UniRef:UniRef50_Q9PRC7", "UniRef:UniRef90_Q9PRC7" ], "nt": "ATGTCAACAATTGTTGCTTTAGCAACCGCGCCTATGAATTGTGCCATTCACATTATTCGGGTTAGTGGTCCAAAAGCTTTTGAAATAATTAATAAAATTTCAACAATAAAAATAAAAAAAGAAACTTTTAAAATTTGGTATACAATTCTAAAAGATGATAATCAAATTTTAGATGAAGTTTTAATAAATACATTTGTAGCTCCAAAAACATTTACTGGAGAAGATTTAGTAGAAATTAATTGCCACGGAGGAATAGTGGTTGCTAATTTAATTATTAAAACATTAATTAAATATGGTTGTCAACCAGCACAACGTGGAGAATTTTCTCGCCGTGCTTTATTAAATAAAAAAATGGATTTATCAAAAATTGAAGCAATTAACAATTTGGTTAATGCTAAAAATGAATTAAGCGTTAAAGGTGTAATTGGCGCTCTATTAGGACGTGTTTCGCAATCAATTGCTGAATTTAGGCATGAGTTATTTATGATAATTGGGCAAATTGAAGTTAATATTGATTATCCTGAATACGATGATGTTGAACAAGTTGATGCTATTATTTTAAAACAACGTTTATTATCTTTAGATAAAAAAATTACAAAAATTATTGATCAATCAAAAAAATTTTTACCAATCAATAAAGGCATTAGGGTGTTAATAATTGGAAAACCAAATGTAGGTAAATCAACTCTATTAAACGCTTTATGCAATGAACAAAAAGCGATTGTTACAGATATTCCTGGGACAACACGTGATGTTATTGAATCCTCAATTAATATTGATAATATTACTTTAAATATCTTAGATACTGCAGGAATTCATTTAACAAATGATTTTGTTGAAAATTTAGGAATTAATAAAGCCAAAGCATTAATTGATAAAGTTGATTTAATTTTATATTTAATACCTGCAAACGAACAACAAGATTTGGAGTTATATGATTTAATTAAAAAACAAAAACATTTATTAGTTTATACAAAAAAAGATTTAGTTGATCAATACGATGATAAACAAATATATATTAATGCAAAAAATAATGATATTCAGTCATTGATTGATGAAATTAAAAAACTTTTTTATGTTCAAGAGTTTGACAATGCTAACATTGATGTTTTACAATCCCAACGTCAAATTGGTATTTTAGAAAATGTTCATTATTTAATTAATAACGCAATTCTTAATTTAGAAAAAGGGGATACACTTGATTTAATTGTCGCTGATTTAGAATTTTGCAATTTACGTTTAAATGAACTCTTAGGCATAAGTAGCGAATATGATTTTTTAGATGATTTATTTAAAAATTTTTGTATAGGTAAATAA", "aa": "MSTIVALATAPMNCAIHIIRVSGPKAFEIINKISTIKIKKETFKIWYTILKDDNQILDEVLINTFVAPKTFTGEDLVEINCHGGIVVANLIIKTLIKYGCQPAQRGEFSRRALLNKKMDLSKIEAINNLVNAKNELSVKGVIGALLGRVSQSIAEFRHELFMIIGQIEVNIDYPEYDDVEQVDAIILKQRLLSLDKKITKIIDQSKKFLPINKGIRVLIIGKPNVGKSTLLNALCNEQKAIVTDIPGTTRDVIESSINIDNITLNILDTAGIHLTNDFVENLGINKAKALIDKVDLILYLIPANEQQDLELYDLIKKQKHLLVYTKKDLVDQYDDKQIYINAKNNDIQSLIDEIKKLFYVQEFDNANIDVLQSQRQIGILENVHYLINNAILNLEKGDTLDLIVADLEFCNLRLNELLGISSEYDFLDDLFKNFCIGK", "aa_hexdigest": "9eb6fc52831024999b4e6c3ed851a25c", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI0000137467", "ncbi_nrp_id": "WP_006688774.1", "uniref100_id": "UniRef100_Q9PRC7", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000137467", "RefSeq:WP_006688774.1", "UniRef:UniRef100_Q9PRC7" ] }, "ips": { "uniref100_id": "UniRef100_Q9PRC7", "uniref90_id": "UniRef90_Q9PRC7" }, "psc": { "uniref90_id": "UniRef90_Q9PRC7", "gene": "mnmE", "product": "tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE", "ec_ids": [ "3.6.-.-" ], "uniref50_id": "UniRef50_Q9PRC7", "cog_id": "COG0486", "cog_category": "J", "go_ids": [ "GO:0002098", "GO:0003924", "GO:0005525", "GO:0005829", "GO:0006400", "GO:0030488", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PRC7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PRC7" ], "product": "tRNA modification GTPase MnmE" }, "genes": [ "mnmE" ], "id": "NKIECMJPJD_281", "locus": "GCA000828735_00245" }, { "type": "cds", "sequence": "contig_1", "start": 155844, "stop": 156641, "strand": "+", "frame": 3, "gene": "tatD", "product": "putative metal-dependent hydrolase UU017", "db_xrefs": [ "COG:COG0084", "COG:N", "EC:3.1.-.-", "GO:0004536", "GO:0005829", "GO:0046872", "KEGG:K03424", "RefSeq:WP_006688502.1", "SO:0001217", "UniParc:UPI0000139104", "UniRef:UniRef100_Q9PRC8", "UniRef:UniRef50_Q9PRC8", "UniRef:UniRef90_Q9PRC8" ], "nt": "ATGTTAGAAAAAATAAAAATTATTGATACTCATACTCATCCTAATATTGAACCATTAATTGAAGAATTTGATGACATTATCTTTAAATGTTATGAACAAGGTATTGGATTAAATATTGTTGGAGTAGATTTAAAAACTTCACAGACAGCCATTCAACAAGCTAAAAAATTTAAATTTTTGACTTGTAGTGTTGCAATCCATCCTAATGATTTACATAATATAGAAAATGATTTTGTTGCATTAGAAAAATTGATAGCAGAAAATATTGATTACATAAGTTGTATTGGTGAGTGTGGTTTAGACTATTATCATGAACAAAATTATGATCATGATTTTCAAAAACATTGGTTTAAAAAACATATTGAATTGGCTAAAAAGTATCATAAACCATTAATGTTACACATCCGTAATGCACACGATGACGCAATTGCCATCTTAAAAGAATTAGCTGTTCAAGATGTAATAATCCATTGTTTTACAGATAAAAAAGAGTATGCACAACAATATATTGAAATGGGTTATTATATTTCATATCCTGGTGTAATTACTTTTAAACCAACAAAAAATAATCAATTACATGAACTGTATGAGGCGATTAAAATAACCCCACTTGATAAGATTCTAGTAGAAACAGACGCCCCATATTTAACACCAGTTCCTAAACGAGGGCAAACTAACTATCCTTATTATGTATTATATACATCAGCATTTATTGCCAACTTATTAGGCATTACACATGAAAAAATGCAAAAAATCTTATTAGAAAATTCATTAAGAATTCTAAATTTTAAAAAATAA", "aa": "MLEKIKIIDTHTHPNIEPLIEEFDDIIFKCYEQGIGLNIVGVDLKTSQTAIQQAKKFKFLTCSVAIHPNDLHNIENDFVALEKLIAENIDYISCIGECGLDYYHEQNYDHDFQKHWFKKHIELAKKYHKPLMLHIRNAHDDAIAILKELAVQDVIIHCFTDKKEYAQQYIEMGYYISYPGVITFKPTKNNQLHELYEAIKITPLDKILVETDAPYLTPVPKRGQTNYPYYVLYTSAFIANLLGITHEKMQKILLENSLRILNFKK", "aa_hexdigest": "0f0eca39b24e68b9a515d191ebbb879e", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI0000139104", "ncbi_nrp_id": "WP_006688502.1", "uniref100_id": "UniRef100_Q9PRC8", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139104", "RefSeq:WP_006688502.1", "UniRef:UniRef100_Q9PRC8" ] }, "ips": { "uniref100_id": "UniRef100_Q9PRC8", "uniref90_id": "UniRef90_Q9PRC8" }, "psc": { "uniref90_id": "UniRef90_Q9PRC8", "gene": "TatD", "product": "Uncharacterized metal-dependent hydrolase UU017", "ec_ids": [ "3.1.-.-" ], "uniref50_id": "UniRef50_Q9PRC8", "cog_id": "COG0084", "cog_category": "N", "kegg_orthology_id": "K03424", "go_ids": [ "GO:0005829", "GO:0004536", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PRC8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PRC8" ], "product": "Uncharacterized metal-dependent hydrolase UU017" }, "genes": [ "tatD" ], "id": "NKIECMJPJD_282", "locus": "GCA000828735_00246" }, { "type": "cds", "sequence": "contig_1", "start": 156791, "stop": 157276, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAAGCAAAATCAATAAAAAAATCTTGTTTGCATCACTTGCTGGAGTGGTTACATTAACTTCAGTTGCAGCTATTGCTGCATCATGTAATGATAGTAATAAAGATGATGGGAAAACAAATAAAGATGGAAATTATATTTCTAAATTGTCTTTAGAAGATTTTTATGCAAAACCAGCTGGAGATGATAGTTTAGGATATCACAGAACATATAATTCATTATACGATGATGGTGCTCGTATGTTAGGCTTGATTTCATTTAGTCACTCAATTCCAATTAAAGAATATTTTGGTTCAAGCAGCGATAAAAAAGATTTAAGTGCAGTTTTAATTGATGATAAATTTAGTGGAACAGTGGGTAAAGACCGTATTGCGTCTGTCTCATATCGTGTAGATCAAGCAGCATTTTTAACAGGTATTGCTGCGGCGTACTATTTAAATGCTAACCAAAAAACTTTTGCAGCAGATGGAAAACTAACTTGA", "aa": "MKKSKINKKILFASLAGVVTLTSVAAIAASCNDSNKDDGKTNKDGNYISKLSLEDFYAKPAGDDSLGYHRTYNSLYDDGARMLGLISFSHSIPIKEYFGSSSDKKDLSAVLIDDKFSGTVGKDRIASVSYRVDQAAFLTGIAAAYYLNANQKTFAADGKLT", "aa_hexdigest": "5238c7ff71855df8bc27f2d6d6540aa3", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17331.307099999984, "isoelectric_point": 8.821547508239746 }, "id": "NKIECMJPJD_283", "locus": "GCA000828735_00247" }, { "type": "cds", "sequence": "contig_1", "start": 157415, "stop": 158032, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGTTGAACAAGTATTTGCTAGCAAATACGTTGCTGGTTCATTTAAACCAGATGAAGAAGGTGCAACAAACATTATCAATGATTTAATTACAAAAAAAGCTGATGTTATTTTACCTGTAGCTGGTCCACAAACAAACCTTGCAACTTCAATTGTTTCAAATGCAACAGATCCAAGTGTAATTATTGGTGTTGATACTGCACAAGAATTAGATGATGTAACAAACCGTAAAAGAATTACAAACAAAACTGTTAATGATGGAAAAACAATTTTATTCTCAATTGTTAAACGTGTTGATTTAGCAATGAAGGGAGCAATTGAAAATGCTTCAAAAGGTGCACAACTAACTAATGATATTAATAAAGATGCTTATAAATTAGGAACACATACTGAAGCAAGTTTAGATAAGAGCACATATGTAGATGATACTCCGCTTGTTGAATTATCAAATGCAGGTCGTGTTTATTTAGAACAAGCGGCTAAACTAGCTGGTTTAAAAGCAATTACATATGCTCAAATTGTTAATGTTATTCAAAATGAAGAATTATTTAAACTTCTTTCAACAAAAGGAACTACAAAATTAGAAGATCTTGCTACAAAAACAAGCGATGGATGA", "aa": "MNVEQVFASKYVAGSFKPDEEGATNIINDLITKKADVILPVAGPQTNLATSIVSNATDPSVIIGVDTAQELDDVTNRKRITNKTVNDGKTILFSIVKRVDLAMKGAIENASKGAQLTNDINKDAYKLGTHTEASLDKSTYVDDTPLVELSNAGRVYLEQAAKLAGLKAITYAQIVNVIQNEELFKLLSTKGTTKLEDLATKTSDG", "aa_hexdigest": "6e7b4fd16eff4a41be83f7d80204441e", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 22001.639900000002, "isoelectric_point": 5.132754325866698 }, "id": "NKIECMJPJD_284", "locus": "GCA000828735_00248" }, { "type": "cds", "sequence": "contig_1", "start": 158421, "stop": 160028, "strand": "+", "frame": 3, "gene": "nupO", "product": "Methylgalactoside permease ATP-binding protein", "db_xrefs": [ "COG:COG3845", "COG:F", "RefSeq:WP_006688670.1", "SO:0001217", "UniParc:UPI00000C1B68", "UniRef:UniRef100_Q9PRD0", "UniRef:UniRef50_P47365", "UniRef:UniRef90_Q9PRD0" ], "nt": "ATGGAAATTAAGACAAATCAATGTCCTTACGCCATAGAAATGCACGAGATTACAAAAACTTTCCTGAACGGGACTGTTATTGCTAATAAAGATGTTAATCTTTTTGTTAAAAAAAATGAAATTCATGCAATTATTGGTGAAAATGGAGCAGGTAAATCAACCTTAATGTCAATTTTATTTGGGATCTATAAACAAGATTCTGGATCAATTAAAATTAATGGTCGAATTGTCAATTTTAGTTCAGCTAAAGATGCTTCAAAAACCGGAATTGGAATGGTACATCAACATTTTAAATTAATTGACACCTTAAGTGTTTTAGATAATGTTATTTTAGGTTCTGAAGGTGCAACGCAACTAGGTATCATTAACCGTAAAAAAATTACTAAAGAACTTAAAAAAATCATTCAAGAATATGGATTAAATATCAATTTAAAAAGTAAAATTTCAAAAATTACAGTTGGTCAACAACAAAAAACGGAAATTTTAAAATTATTATATCGTGATATTGATATTTTAATTTTTGATGAACCAACTGCTGTTTTGAGTGAAGATGAAATTCAAGCATTTTTACAAATGCTAAAAGATTTTAAAGCAGCAGGCAAGACAATCATTATTATTACGCATAAACTAAATGAAATTAAAGAAGTTGCTGATTCTGCAACAGTTATTCGTCGCGGACATTATATTGATAGTTTTGATGTTAAACAAAAAACAATAGCTGAAATGGCTGAATTAATGGTTGGTCGAAAATTGGTTGAAGTTAAAAATATCGATCCAATCACAAGTAATGAAAAAGTTTTCGAAGTTCAAAATTTAGATATTCAATCAATTGTTAAAAAACAAAAAGATATTGTGAAAGTTAAATCATCACCAAAAGGTGATGATGTTTCATTGTTAAGTACTAAAAATAATAATTTTATTAATTTTAGTATCCGCAAGGGTGAAATTTTTGCTATTGCCGGTGTTGAAGGTAATGGTCAAAGTGAATTAGCGCAAATAATCTCTGGCTTATTAAAAGGAAATAAGGAAGCAAAAATTATGCTTGATAATCAAGAAATTGAACATGCAAGCATTAGAAAACGTTATCAGTTAGGATTATCACATGTACCAGAAGATCGTCATAAACATGGATTAGTTTTAGATGACACGATTGCTATGAATGCGATCTTACAACAAGTTGATGATAAACCATATAGTAATTTAGGATTTTTTAATAATAATGAAATTTCAAAACATGCAATTAATATTATTAAAAAATATGATGTTCGTGGTACAACACGTGGTACATCAGATGCACGAGGATTATCTGGTGGAAACCAACAAAAACTAATTATTGGTCGTGAATTTGAACGATCTCATAAATTAATTTTATTGGTTCAACCAACAAGAGGTTTAGATTTAGGTGCGATTGAATTTATTCATGAACAAACATTAGAAGAGAAACGAAAAGGTAATGCTATTTTATTAATTTCTTACGAATTAGACGAAATTTTATCACTTGCTGATACAATTGCTGTAATTCATAATGGATATTTCATTAGTGTTGGTGATCGTTTTTCAATGACAAGACAAAAAATTGGTGAATTAATGGCAGGTGAAAAAATATAA", "aa": "MEIKTNQCPYAIEMHEITKTFLNGTVIANKDVNLFVKKNEIHAIIGENGAGKSTLMSILFGIYKQDSGSIKINGRIVNFSSAKDASKTGIGMVHQHFKLIDTLSVLDNVILGSEGATQLGIINRKKITKELKKIIQEYGLNINLKSKISKITVGQQQKTEILKLLYRDIDILIFDEPTAVLSEDEIQAFLQMLKDFKAAGKTIIIITHKLNEIKEVADSATVIRRGHYIDSFDVKQKTIAEMAELMVGRKLVEVKNIDPITSNEKVFEVQNLDIQSIVKKQKDIVKVKSSPKGDDVSLLSTKNNNFINFSIRKGEIFAIAGVEGNGQSELAQIISGLLKGNKEAKIMLDNQEIEHASIRKRYQLGLSHVPEDRHKHGLVLDDTIAMNAILQQVDDKPYSNLGFFNNNEISKHAINIIKKYDVRGTTRGTSDARGLSGGNQQKLIIGREFERSHKLILLVQPTRGLDLGAIEFIHEQTLEEKRKGNAILLISYELDEILSLADTIAVIHNGYFISVGDRFSMTRQKIGELMAGEKI", "aa_hexdigest": "64fc131700809a1f1d080d1dd9eeaddc", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00000C1B68", "ncbi_nrp_id": "WP_006688670.1", "uniref100_id": "UniRef100_Q9PRD0", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1B68", "RefSeq:WP_006688670.1", "UniRef:UniRef100_Q9PRD0" ] }, "ips": { "uniref100_id": "UniRef100_Q9PRD0", "uniref90_id": "UniRef90_Q9PRD0" }, "psc": { "uniref90_id": "UniRef90_Q9PRD0", "gene": "NupO", "product": "Methylgalactoside permease ATP-binding protein", "uniref50_id": "UniRef50_P47365", "cog_id": "COG3845", "cog_category": "F" }, "pscc": { "uniref50_id": "UniRef50_P47365", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47365" ], "product": "Putative carbohydrate transport ATP-binding protein MG119" }, "genes": [ "nupO" ], "id": "NKIECMJPJD_285", "locus": "GCA000828735_00249" }, { "type": "cds", "sequence": "contig_1", "start": 160031, "stop": 160216, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGAAATTTAAAACTCTTTTAAGTTACGATAGCTTTCTTAAAGGTCAAGAGCGAAAAACAATCACTAAAAAATTCTTATCGACAGTTTTTGCCATTTTTGTAGCGATAGTAGTTTCAGCTATTTTGGTTGGAATATTAGGCTATAACATACGTGATTTCTTTATAAGATTATTTACTAAATGA", "aa": "MLKFKTLLSYDSFLKGQERKTITKKFLSTVFAIFVAIVVSAILVGILGYNIRDFFIRLFTK", "aa_hexdigest": "2a313d487e1ad3476f72e27e2206998f", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7051.467199999999, "isoelectric_point": 10.288783454895018 }, "id": "NKIECMJPJD_286", "locus": "GCA000828735_00250" }, { "type": "cds", "sequence": "contig_1", "start": 160328, "stop": 160525, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAGGTTCAGGTTTGATTATGTTATTAGTCGTAAAAAACATGCAAATTGCCAATATTAATATGCCTAATGTTTTAGGTCAATTTTTCTTATTAATCATTGCAATGTTAGCAGGAGCAATGTTTGCAGTTTTTATAGGAATTTTAAAAGTATTTTTAAAGATTAATGAAGTTGTTAGTTCAATTTTATTGAATTGA", "aa": "MLGSGLIMLLVVKNMQIANINMPNVLGQFFLLIIAMLAGAMFAVFIGILKVFLKINEVVSSILLN", "aa_hexdigest": "1b88a877773901de8b4c0f7c75d2489b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7064.784899999997, "isoelectric_point": 9.700251579284668 }, "id": "NKIECMJPJD_287", "locus": "GCA000828735_00251" }, { "type": "cds", "sequence": "contig_1", "start": 160529, "stop": 160711, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTCTATATTACGCGTTATGTTGTTTTTACTGCACAAAGTAAAATGTATAATCCAACACCAGATCAAGTTGGATCACGATCAATTCCTTTTGCTGATAATTATTCTTTATTTCATTTAGCATCTGGATATGGTTATTTATTTGCTTTATTGATCTTTATTTCTTTATCTATTATTATTTGA", "aa": "MFYITRYVVFTAQSKMYNPTPDQVGSRSIPFADNYSLFHLASGYGYLFALLIFISLSIII", "aa_hexdigest": "a6b0bddab2971659c7475cb9e5cd007a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6862.916399999996, "isoelectric_point": 8.084029197692871 }, "id": "NKIECMJPJD_288", "locus": "GCA000828735_00252" }, { "type": "cds", "sequence": "contig_1", "start": 160856, "stop": 161134, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTAATTATACTTCAACAACAACAAATGCGATTGTTATTAGTCAAGCTGCTGATGCCTTACCAACCCAAGGATATGATGGAATTGCGATGGGATTAATTTCTCTAGCACATCCTTTAGCAACAATTCCTGTCTCATTCTTAATGGGATTATTACAACAATCTGCTGATAATTTAGGAGGAAGTTTTCCACAAGATTTATCTGGAATTATTATTTCATTTGTAATGTTAGGGGCAGCAATGTTTATTTTATTTGAACGAATAAGTCCCGTTTATTGA", "aa": "MVNYTSTTTNAIVISQAADALPTQGYDGIAMGLISLAHPLATIPVSFLMGLLQQSADNLGGSFPQDLSGIIISFVMLGAAMFILFERISPVY", "aa_hexdigest": "46117efb0bc601b61f565408b36e4289", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9680.175399999996, "isoelectric_point": 4.0500284194946286 }, "id": "NKIECMJPJD_289", "locus": "GCA000828735_00253" }, { "type": "cds", "sequence": "contig_1", "start": 161432, "stop": 162178, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAACTTAAAAAACATTTAATTATTGAAAGAGCAACACTTGTTTATTATCCAGAAATTAACACAACAAGAAAAATTAAAAATAAACTAAAACATTTTGAAAACATAAGCGCTAAAAAACTAAGTCGTATTAAAGACAAAATTCATAATGAAGATTTTTTAATTAAAAATTTAGTGGCTAAAGAAATTAGTAAATATGCTCAATCAAAATCGTTTGATTATAATAAGCTAAAATATTTATTACAAGAATATGAAAATAACAATTCAGTGTTAACAACCGAGTTAGTTAATGAGTTATCTTTAGTTCTAAAAGATGAACAAGCACAATTAATGTTTAGTGAATTTAAACAGGCAAGTTTTGAGAATAATTTAGATCACATGATTGATTTAAAAAATCAAATTATGACATACATTAATTCTTTATTAGATTCTCGATTAGAACAATTGTCAATAACTTTAAAATCAAAGAATTTAGTTCATGATGAACAAATGGATAAGATTATTTTATTACTAAATCCAAATGATAAAATTCTTAATAAAATTATTATTTCGCGTAATAAACATTTAGAAAATTACACGAAAGAAATTACAAAATTAGAATTAAGAAATATTTCGCAAGATGATGAAATTAAAAAGAATACAAAACAACGCTCATCATCATACTTGACAAAACATTATGAAAAAACTAAACAACATATTAATAAAATTAATCTAGATCAAGAACAAAAATTATTATTAGAAGATTGA", "aa": "MQLKKHLIIERATLVYYPEINTTRKIKNKLKHFENISAKKLSRIKDKIHNEDFLIKNLVAKEISKYAQSKSFDYNKLKYLLQEYENNNSVLTTELVNELSLVLKDEQAQLMFSEFKQASFENNLDHMIDLKNQIMTYINSLLDSRLEQLSITLKSKNLVHDEQMDKIILLLNPNDKILNKIIISRNKHLENYTKEITKLELRNISQDDEIKKNTKQRSSSYLTKHYEKTKQHINKINLDQEQKLLLED", "aa_hexdigest": "2837a0d4742dbcae5988e39c05c6a6d5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 29505.681700000074, "isoelectric_point": 9.187212181091308 }, "id": "NKIECMJPJD_290", "locus": "GCA000828735_00254" }, { "type": "cds", "sequence": "contig_1", "start": 162241, "stop": 162840, "strand": "+", "frame": 1, "gene": "nupQ", "product": "Ribose/galactose ABC transporter", "db_xrefs": [ "COG:COG1079", "COG:F", "KEGG:K23536", "SO:0001217", "UniRef:UniRef50_Q9PRD2", "UniRef:UniRef90_Q9PRD2" ], "nt": "ATGCCTTCATTATCATTAATTAACGATGCCTCTATTTACGCTGCTATTTTAATTCTAGGTGCGTTAAGTGGTTTCTTTTGTGAACGTGTTGGGATTGCTAATATTTCAATTAATGGTCAAATGATTATTGGTGCCTTAGTCTTTACATTATTTTCAACAATTATTTATAAATATATGGGAACAAAAACGGAAACAGAATATACATTTATTGTATGTTTAATTATTGCTGGAATATTGACTATTCCAACTTCATTATTATTTGGTTTTTTAACTATAAAATTAAAAACCAATCAAATTATTGCTGGAACAGCTATTAATTTATTAGCTTCAGGAGTAGCAACCTTTGTAACTTATCCTTTGGGCGATAAAATTGCTGACAAAACAAGTCTATCTTCTGATTATTTAGGATTAATGAAAATTGGTGATGGAATCAACACTATTTATATTGGAAGTATTTTAATTTTAATTGCTGTTGTTTTAATTACCATTGGTTTAATGATAATGATGAAAAAAACTCCATTTGGTTTAAGATTATATGCAATTGGTGAAAACCCAAATGCAGCAGATGCACAAGGCATTAATGTTTATAAATACCAATGA", "aa": "MPSLSLINDASIYAAILILGALSGFFCERVGIANISINGQMIIGALVFTLFSTIIYKYMGTKTETEYTFIVCLIIAGILTIPTSLLFGFLTIKLKTNQIIAGTAINLLASGVATFVTYPLGDKIADKTSLSSDYLGLMKIGDGINTIYIGSILILIAVVLITIGLMIMMKKTPFGLRLYAIGENPNAADAQGINVYKYQ", "aa_hexdigest": "b9764402833ba191976f1276b8c97d6c", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "genes": [ "nupQ" ], "truncated": "3-prime", "pseudogene": { "start": 162241, "stop": 163143, "observations": { "mutation": [ 162838, 162973 ], "stop": [ 162838, 162973 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PRD2", "query_cov": 1.0, "subject_cov": 0.6440129449838188, "identity": 0.985, "score": 365.0, "evalue": 1.16e-125, "gene_start": 1, "gene_end": 199, "reference_start": 1, "reference_end": 199, "reference_sequence": "MPSLSLINDASIYAAILILGALSGFFCERVGIANISINGQMIIGALVFTLFSTIIYKYMGTKTETEYTFIVCLIIAGILTIPTSLLFGFLTIKLKTNQVIAGTAINLLASGVATFVTYPLGDKIANKTSLSSDYLGLMKIGDGINTIYIGSILILIAVVLITIGLIIMMKKTPFGLRLYAIGENPNAADAQGINVYKYQWIAVSISGFIAGIGGGLYMYASRSPFGGEVGGIGFLALAILIAGAWRIPLIVVVSIAFAVITKTFSQIDKIPQEIGKLIPYVITLIALISFSKYSVAPKNVGIPFDKSKR" }, "paralog": false, "identity": 0.9766666666666667, "score": 555.0, "evalue": 3.55e-203, "description": "Internal stop codon at 162838, 162973. Nonsense mutation around 162838, 162973.", "psc": { "uniref90_id": "UniRef90_Q9PRD2", "gene": "NupQ", "product": "Ribose/galactose ABC transporter", "uniref50_id": "UniRef50_Q9PRD2", "cog_id": "COG1079", "cog_category": "F", "kegg_orthology_id": "K23536", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PRD2", "UniRef:UniRef50_Q9PRD2", "COG:COG1079", "COG:F", "KEGG:K23536" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PRD2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PRD2" ], "product": "Ribose/galactose ABC transporter" } }, "id": "NKIECMJPJD_291", "locus": "GCA000828735_00255" }, { "type": "cds", "sequence": "contig_1", "start": 162825, "stop": 162956, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTAAAGCTAAGAACCCGATCCCACCAACCTCGCCTGCAAATGGAGAACGTGATGCATACATATATAATCCACCACCAATTCCAGCAATAAAACCAGAAATCGAAACAGCAATTCATTGGTATTTATAA", "aa": "MAKAKNPIPPTSPANGERDAYIYNPPPIPAIKPEIETAIHWYL", "aa_hexdigest": "94730e08232a40bf283c56f485bd36d9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4786.463699999999, "isoelectric_point": 6.523149681091309 }, "id": "NKIECMJPJD_292", "locus": "GCA000828735_00256" }, { "type": "cds", "sequence": "contig_1", "start": 163556, "stop": 164014, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAGTTAAATAAAAAAGTTCTATTTTTAGCAATTGGTAGCGTTTTTGCTTTAGGTACAATTATTACTGTAGCAACATCATGTACACAAAAATCTACATTAAACTATAGTCAATTTTATTGGACTTCACCAACAAGTGATGATGATGAAGGTTTTCAAACAAAATATAAAAGTATGGCTAGTGATGGTAAACGAGCTTTATTAATGCCTGGTTATCAACATCCTGAAAGATTACAAAATGCTTTAGTAAATGATAAATTTGATCCTAATTTAATCGCTTTAATTTTAGATGGAGTTTATAATAATGACAATAAAGCTGAGTTTTATAAGGGAGCAGATCGTGTTGCTGATGTTTATTTTAAAGTTGATGAAGCCGCTTTTTTAGGAGGTATTGCTGCAGCTTATATGTTAAACTCAAATCAAGCAGTTTTTGGTGCAGATAATAAATTAACTTGA", "aa": "MKKLNKKVLFLAIGSVFALGTIITVATSCTQKSTLNYSQFYWTSPTSDDDEGFQTKYKSMASDGKRALLMPGYQHPERLQNALVNDKFDPNLIALILDGVYNNDNKAEFYKGADRVADVYFKVDEAAFLGGIAAAYMLNSNQAVFGADNKLT", "aa_hexdigest": "270c2e645a2451bb70b32c12aa027ea8", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16784.86429999998, "isoelectric_point": 6.559412956237793 }, "id": "NKIECMJPJD_293", "locus": "GCA000828735_00257" }, { "type": "cds", "sequence": "contig_1", "start": 165331, "stop": 165696, "strand": "-", "frame": 3, "gene": "rplL", "product": "50S ribosomal protein L7/L12", "db_xrefs": [ "COG:COG0222", "COG:J", "GO:0003729", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625", "RefSeq:WP_006688489.1", "SO:0001217", "UniParc:UPI00001341D0", "UniRef:UniRef100_Q9PRD4", "UniRef:UniRef50_Q9PRD4", "UniRef:UniRef90_Q9PRD4" ], "nt": "ATGTCAAAATTAACAATTGAACAATTTATTGCTGCGATTAAAGAAATGTCAATGCTAGAATTAAACGATTTGGTTAAAGCTATCGAAACTGAATTTGGTGTGTCTGCTGCTGCTCCTGTTGCCGCTGCTGCTGCTCCTGTTGCCGCTGAAGCTCCAACTGAAGTAACTATTAAATTAGTTGAAGCTGGTACTAATAAAGTTGGTGTAATTAAATTAATTCGTGAGATTACAGGATTAGGATTAATGGAAGCTAAAACTGCTGCTGAAACTGCAGGCAGTGTAATTAAAGAAGATGTTAAAACAGAAGAAGCTAACGAAATCAAAAAGAAATTTGATGAACTTGGTGCAAAAGTACAACTTGTTTAA", "aa": "MSKLTIEQFIAAIKEMSMLELNDLVKAIETEFGVSAAAPVAAAAAPVAAEAPTEVTIKLVEAGTNKVGVIKLIREITGLGLMEAKTAAETAGSVIKEDVKTEEANEIKKKFDELGAKVQLV", "aa_hexdigest": "098fdee1bb231bf865286496741d2ff6", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI00001341D0", "ncbi_nrp_id": "WP_006688489.1", "uniref100_id": "UniRef100_Q9PRD4", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001341D0", "RefSeq:WP_006688489.1", "UniRef:UniRef100_Q9PRD4" ] }, "ips": { "uniref100_id": "UniRef100_Q9PRD4", "uniref90_id": "UniRef90_Q9PRD4" }, "psc": { "uniref90_id": "UniRef90_Q9PRD4", "gene": "rplL", "product": "50S ribosomal protein L7/L12", "uniref50_id": "UniRef50_Q9PRD4", "cog_id": "COG0222", "cog_category": "J", "go_ids": [ "GO:0003729", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PRD4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PRD4" ], "product": "Large ribosomal subunit protein bL12" }, "genes": [ "rplL" ], "id": "NKIECMJPJD_294", "locus": "GCA000828735_00258" }, { "type": "cds", "sequence": "contig_1", "start": 165721, "stop": 166221, "strand": "-", "frame": 3, "gene": "rplJ", "product": "50S ribosomal protein L10", "db_xrefs": [ "COG:COG0244", "COG:J", "GO:0003735", "GO:0006412", "GO:0015934", "GO:0070180", "RefSeq:WP_006688636.1", "SO:0001217", "UniParc:UPI0000133B67", "UniRef:UniRef100_Q9PRD5", "UniRef:UniRef50_Q9PRD5", "UniRef:UniRef90_Q9PRD5" ], "nt": "ATGGCAAATGTTAGACCAAGTGTTGTTTTTAAACAACAAGAAGTCAACCATATGGCAGATATACTAAAAAATTCAAAATCTTTTATTGTTTTTGAATATCATGGATTAACTGCTGCTAACATTTTAGCGTTAAGAAATGTTTTACATTCATCAAATTCTAAATTATATGTTTTAAAAAATAATATTACAGCACGTGCTTTTGAAAAAGCTGGTGTTAGTGGATTTGAAAATCAATTAACAGGACCTAACGCAGTTGCTGTTGCAATGGACGATGAAATTGCTGCGATTAAAGCAGTAAATGATGTAGCTAAAGAATTTGATTTTGTCAAAATTAAAGGTGCATATCTTGAAAACAAATTTGCAGATACTCACAAAATCGATCAACTTGCTGCAATTCCTGGCCGTGAAGGTCTATACTCAATGCTACTTTCTTGCTTCACAGCACCATTACGTAATGTGATGTATGGTTTAAAAGCTGTAGCTGAGCAAAAAGGTGAATAA", "aa": "MANVRPSVVFKQQEVNHMADILKNSKSFIVFEYHGLTAANILALRNVLHSSNSKLYVLKNNITARAFEKAGVSGFENQLTGPNAVAVAMDDEIAAIKAVNDVAKEFDFVKIKGAYLENKFADTHKIDQLAAIPGREGLYSMLLSCFTAPLRNVMYGLKAVAEQKGE", "aa_hexdigest": "1637497bdbaf949a27840b3c816cf6b6", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI0000133B67", "ncbi_nrp_id": "WP_006688636.1", "uniref100_id": "UniRef100_Q9PRD5", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000133B67", "RefSeq:WP_006688636.1", "UniRef:UniRef100_Q9PRD5" ] }, "ips": { "uniref100_id": "UniRef100_Q9PRD5", "uniref90_id": "UniRef90_Q9PRD5" }, "psc": { "uniref90_id": "UniRef90_Q9PRD5", "gene": "rplJ", "product": "50S ribosomal protein L10", "uniref50_id": "UniRef50_Q9PRD5", "cog_id": "COG0244", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015934", "GO:0070180" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PRD5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PRD5" ], "product": "Large ribosomal subunit protein uL10" }, "genes": [ "rplJ" ], "id": "NKIECMJPJD_295", "locus": "GCA000828735_00259" }, { "type": "cds", "sequence": "contig_1", "start": 166435, "stop": 167082, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTTCAGCTACATGTACTGGTTTTTTAATTGATAAATTTCAAGATGTAGGACAATTAGTCACAGCAGGTTTATCAACATTAGTTATCGTACCAATCATTCCAGCAATGACGGGAACAAGTGGAAATGCTGGATCACAAGCTGCCGCCTCTGTGATTCGTGCTTTATCAGTTGGTGAAATTACTTTAAAAGAATATAATAAAGTCCTTGCCAAAGAATTTTTGGTTGGTGCTTTAATTGGTTTAGTTTTAGCAATTATTAATTTTGCTCGTTTGATTATATATTTTGCTATTATTAAACCTGATTTACAACAATACAATGTTTTATACGAAAATATTACTAATAATCCACATACTAAAATGATTGTAGGAGCAATTGTTTCAGCTGGATCATCAGTCGCTTTGTTTTTTGCTATTGTAATTTCTAAATTGCTTGGTGGAACACTGCCACTTTTAGCAACAAAATTAAAAATTGATCCTACAATTATGTCAACACCAATTTTATCGACATTACTTGATATGGTTACAACTATTATCCTATTTGGTTTTGGTATTATATTTTTATTAATTATTGTTGATAAAGTTAAAGTCGAACAACTAAATAATATCCGCGAGCATATTCATGCTATTAAAAATGTTAATTCATGA", "aa": "MISATCTGFLIDKFQDVGQLVTAGLSTLVIVPIIPAMTGTSGNAGSQAAASVIRALSVGEITLKEYNKVLAKEFLVGALIGLVLAIINFARLIIYFAIIKPDLQQYNVLYENITNNPHTKMIVGAIVSAGSSVALFFAIVISKLLGGTLPLLATKLKIDPTIMSTPILSTLLDMVTTIILFGFGIIFLLIIVDKVKVEQLNNIREHIHAIKNVNS", "aa_hexdigest": "79e62f7196c9b2c48e1e908f66da3515", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 23004.234500000028, "isoelectric_point": 9.015017127990724 }, "id": "NKIECMJPJD_296", "locus": "GCA000828735_00260" }, { "type": "cds", "sequence": "contig_1", "start": 167095, "stop": 168000, "strand": "-", "frame": 3, "gene": "mgtE", "product": "magnesium transporter", "db_xrefs": [ "COG:COG2239", "COG:P", "GO:0015095", "GO:0015693", "GO:0016020", "SO:0001217", "UniRef:UniRef50_A0A084F1J3", "UniRef:UniRef90_Q9PRD6" ], "nt": "GTGGAAAATAAACAAAGTACATTAAATTTAAATAACAACACAAATATTAATCAAAATTTTCTAACACAAAATATTTTAAAAACTTACGAACATAAAGATATTACAGGACTTAAAAAATTACTAAAATCAAAACGGATTGATAAAATTGTTGAAAGTCTTGAAACTATTAATGATACACAAATTATTTTATTTGTATTAGTTGCCACAAAAGATGGAATTTGTGGTGATATTTTTAAATATTTAAATACTGAATTAAAATCAAAAATCATAAATGACGCAAGTTTACAGCAATTAAGAATCATTTTATTTGAACTTTATAATGATGATGTGATTATTTTAAAAAATGATTTTCCTATGTATACTAAAAAAATCTTACTTTCATTAGACAGCCAACAAAGAGCAGCTATTAAGCAATTATCTGAATTTGATGAAGATGAAGCTGGTTCAATCATCAATTCTGACTTTTTTACAATCAATCAAAATCTTTCTATCAAAGAAGCACTAATTGAAATAAAAAGAATGTATAATGACTTTGAACAAAGTAATATTATATATGTAGTAGATAATTATAATCGTTTAAAAGGATATGTAACAATTCATTCATTACTTTTTGCAGATTCTTTTGAATTGAAAATTTCTAATGTTATTCAAGAAGATGTTTTCTATGTTCGTAGTGATGAAGATATTGATGCTGTTTTAGATATTTTCAGAAAGTATCAAATTGAACAATTAGCAGTTGTTGATAAAGATAACCAATTAATTGGTTATATTAGTGATAATGATATTTTACCCGTAATTAATGCTGAAACAACTACTGATATTTATAAAATGTATGGGATTAGCGAATTAGATTTTCCTTACATTAAATCAAGTGTTTTTGTTTTATTTAAATCACGTCTATTATGA", "aa": "MENKQSTLNLNNNTNINQNFLTQNILKTYEHKDITGLKKLLKSKRIDKIVESLETINDTQIILFVLVATKDGICGDIFKYLNTELKSKIINDASLQQLRIILFELYNDDVIILKNDFPMYTKKILLSLDSQQRAAIKQLSEFDEDEAGSIINSDFFTINQNLSIKEALIEIKRMYNDFEQSNIIYVVDNYNRLKGYVTIHSLLFADSFELKISNVIQEDVFYVRSDEDIDAVLDIFRKYQIEQLAVVDKDNQLIGYISDNDILPVINAETTTDIYKMYGISELDFPYIKSSVFVLFKSRLL", "aa_hexdigest": "88aa72bf53caf8f0b46be4b30de05c60", "start_type": "GTG", "rbs_motif": "GGAGG", "genes": [ "mgtE" ], "truncated": "5-prime", "pseudogene": { "start": 166792, "stop": 168000, "observations": { "mutation": [ 167097 ], "stop": [ 167097 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PRD6", "query_cov": 1.0, "subject_cov": 0.5574074074074075, "identity": 0.924, "score": 534.0, "evalue": 3.61e-187, "gene_start": 1, "gene_end": 301, "reference_start": 1, "reference_end": 301, "reference_sequence": "MKNKQNTSNLNNNTNINQNFLSQNILKAYEHKDIAGLKKLLKSKRIDKIVESLEAIDDTQIILFVLVATKDGICGEIFKYLNTELKSKIIDDASLQQLRIILFELYNDDVTILKNDFPMHTKKILLSLDSQQRAAIKQLSEFDEDEAGSIVNSDFFTINQSISVKEALIEIKRMYNDFEQSNIIYVVDDYNRLKGYVTIHSLLFADSFDLKISSVVQEDVFYVRSDEDIDAVLDIFRKYQIEQLAVVDKNDQLIGYISDNDILPVINTETTTDIYKMYGISELDFPYIKSSVFVLFKSRLLWLAVLMISATCTGFLIDKFQNVGQLVTAGLSTLVIVPIIPAMTGTSGNAGSQAAASVIRALSIGEITTKEYNKVLAKEFLVGALIGLVLAVINFARLIIYFAIIKPDLQQYNVLYNNITNNPHSQMIVGAIVSAGSSVALFFAIVISKLLGGILPLLATKLKIDPTIMSTPILSTLLDMVTTIILFGFGILFLLIIVDKVKVEELNNMRESIHAVKNINSLLPKQNFLLNNSNYLNHLA" }, "paralog": false, "identity": 0.927860696517413, "score": 723.0, "evalue": 5.72e-264, "description": "Internal stop codon at 167097. Nonsense mutation around 167097.", "psc": { "uniref90_id": "UniRef90_Q9PRD6", "gene": "mgtE", "product": "magnesium transporter", "uniref50_id": "UniRef50_A0A084F1J3", "cog_id": "COG2239", "cog_category": "P", "go_ids": [ "GO:0015095", "GO:0015693", "GO:0016020" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PRD6", "UniRef:UniRef50_A0A084F1J3", "COG:COG2239", "COG:P", "GO:0015095", "GO:0015693", "GO:0016020" ] }, "pscc": { "uniref50_id": "UniRef50_A0A084F1J3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A084F1J3" ], "product": "Magnesium transporter MgtE" } }, "id": "NKIECMJPJD_297", "locus": "GCA000828735_00261" }, { "type": "cds", "sequence": "contig_1", "start": 168185, "stop": 168445, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAGTATTCTAATTCAGTCATACCAGCGGCTTTAGCTTTAGCTTTATTTGCAATTTTTAAAGCAAACACAATTATACTTGCAATGCAAGATAGTGTTTCACTTAGAGCTACAAGAATAAATCCTCCAGGGTTTACAGAAACACCTAATCAAGCAAATATCGCTAATAATCCTAAACCAGCAATTGAAATAATTCACATACCAATAGCCAAATTTTCAGTTCTTTTTGAAATTAGTACTTTAATAACAGCAGGTAAATAA", "aa": "MQYSNSVIPAALALALFAIFKANTIILAMQDSVSLRATRINPPGFTETPNQANIANNPKPAIEIIHIPIAKFSVLFEISTLITAGK", "aa_hexdigest": "523f5912c69bf3b70bb2504281847995", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9234.738499999994, "isoelectric_point": 9.397636985778806 }, "id": "NKIECMJPJD_298", "locus": "GCA000828735_00262" }, { "type": "cds", "sequence": "contig_1", "start": 168490, "stop": 168621, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATTTCACTTCATTATTGCAAGATGGTATATATGAAGTTGGTAATGGTGCAATCGTTACTGACCAATCACCATATTTAGGAATTACACCTGATTATCAGGGTGCATATGGTTTCCCAACACACCCTTGA", "aa": "MNFTSLLQDGIYEVGNGAIVTDQSPYLGITPDYQGAYGFPTHP", "aa_hexdigest": "1b8e452754c6341c71c69dbfde599fe4", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4649.062200000002, "isoelectric_point": 4.0500284194946286 }, "id": "NKIECMJPJD_299", "locus": "GCA000828735_00263" }, { "type": "cds", "sequence": "contig_1", "start": 168624, "stop": 168773, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGTGAAGCATTATGTTCAGTAACTTCATTTATTGTTCTATTCTGCAAAGTTAATAACATGATTAAAGCTAAAAAAATGGGAATGAGCGAAGAAGAATATTATGAAAAACAAATTAAACCATTCCTAAAAGTGAAAGGAGCTAACTAA", "aa": "MGEALCSVTSFIVLFCKVNNMIKAKKMGMSEEEYYEKQIKPFLKVKGAN", "aa_hexdigest": "8a1b0daa51d203c93f6cf16ae31a8441", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5577.646500000001, "isoelectric_point": 9.032230186462403 }, "id": "NKIECMJPJD_300", "locus": "GCA000828735_00264" }, { "type": "cds", "sequence": "contig_1", "start": 168810, "stop": 169184, "strand": "-", "frame": 1, "gene": null, "product": "putative protein UU007", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_Q9PRD8", "UniRef:UniRef90_Q9PRD8" ], "nt": "ATGTTAAATATTTTAAATAATGTATCTAATTCTTCACTATATTCAGCCGCTTCAAATGCTACTGCGCAAACTGGTTCAAATTTGATCAATGATCTAGTTCCAGAAACATTAACCGCTTCAGGAATTAGTATCGCTATTTCTGTTTTTAGTGTTATAGGTACGATCGTTATTGCTTTGAGTGTATTGCCACAAACAATTAAAACTTTACGTGAAAAAGATACAGCTTCATTAAGTTTATTACTATTCTTGCTTAATGGTATTGCTACAGCCTTTTTAACTCTATACGGTATTGGACTTGTTACAGTTCATCCAAATTCATTTTCATTCTTAGTGGATATTAAAAACGGAATGTTCATTTATAATAGAGAAGAGTGA", "aa": "MLNILNNVSNSSLYSAASNATAQTGSNLINDLVPETLTASGISIAISVFSVIGTIVIALSVLPQTIKTLREKDTASLSLLLFLLNGIATAFLTLYGIGLVTVHPNSFSFLVDIKNGMFIYNREE", "aa_hexdigest": "67735307c07979c082ca8673a87818bf", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 168624, "stop": 169184, "observations": { "mutation": [ 168812 ], "stop": [ 168812 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PRD8", "query_cov": 1.0, "subject_cov": 0.6666666666666666, "identity": 1.0, "score": 219.0, "evalue": 4.15e-71, "gene_start": 1, "gene_end": 124, "reference_start": 1, "reference_end": 124, "reference_sequence": "MLNILNNVSNSSLYSAASNATAQTGSNLINDLVPETLTASGISIAISVFSVIGTIVIALSVLPQTIKTLREKDTASLSLLLFLLNGIATAFLTLYGIGLVTVHPNSFSFLVDIKNGMFIYNREEWVAGYLICGIFLIMGEALCSVTSFIVLFCKVNNMIKAKKMGMSEEEYYEKQIKPFLKVKGAN" }, "paralog": false, "identity": 0.9946236559139785, "score": 353.0, "evalue": 1.87e-126, "description": "Internal stop codon at 168812. Nonsense mutation around 168812.", "psc": { "uniref90_id": "UniRef90_Q9PRD8", "product": "Uncharacterized protein UU007", "uniref50_id": "UniRef50_Q9PRD8", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PRD8", "UniRef:UniRef50_Q9PRD8", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PRD8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PRD8" ], "product": "Uncharacterized protein UU007" } }, "id": "NKIECMJPJD_301", "locus": "GCA000828735_00265" }, { "type": "cds", "sequence": "contig_1", "start": 169280, "stop": 169744, "strand": "-", "frame": 2, "gene": "rpiB", "product": "Galactoside O-acetyltransferase", "db_xrefs": [ "COG:COG0698", "COG:G", "EC:5.3.1.6", "KEGG:K01808", "RefSeq:WP_045470345.1", "SO:0001217", "UniParc:UPI0004565FB2", "UniRef:UniRef100_UPI0004565FB2", "UniRef:UniRef50_Q9PRD9", "UniRef:UniRef90_Q9PRD9" ], "nt": "ATGATAAAAAAAATTTATTTTGGTAATGATCATGCTGCTTATGAAATTAAAGATCAAATTATCACTCATTTAAAACAAAAAGGTTATCAAATTATTGATGAAGGTGCAAAAGCCGCATTAGGTTCTGTTGATTATAGTACATATGCATTAAAAGTTGCTAATGACGTTGTTAATGATACTAAAAATGATAGCATAGGTATTTTATTATGTGGCACGGGAATAGGTATGAATATAGCAGCAAGCAAAGTGCATGGAACGCGGGTTGCGTTAGTGTATAACGAAAGCAGTGCAAAACTAGCCAAGGAGCATAATAATGCTAACATAATTACTATAGGAGCACGTGAAAATAGTTTAAACCAAATTCTTAAAATGATTGATGATTTTTTAACAAGTAAATTTTTAGGCGAGCGTCACCAAAAACGTTTAGATATAATTGCAAAATATGAAAAAGAGCAAAATAGTTAA", "aa": "MIKKIYFGNDHAAYEIKDQIITHLKQKGYQIIDEGAKAALGSVDYSTYALKVANDVVNDTKNDSIGILLCGTGIGMNIAASKVHGTRVALVYNESSAKLAKEHNNANIITIGARENSLNQILKMIDDFLTSKFLGERHQKRLDIIAKYEKEQNS", "aa_hexdigest": "e4976cf6c04775188a74e7c6cac575c0", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI0004565FB2", "ncbi_nrp_id": "WP_045470345.1", "uniref100_id": "UniRef100_UPI0004565FB2", "db_xrefs": [ "SO:0001217", "UniParc:UPI0004565FB2", "RefSeq:WP_045470345.1", "UniRef:UniRef100_UPI0004565FB2" ] }, "ips": { "uniref100_id": "UniRef100_UPI0004565FB2", "uniref90_id": "UniRef90_Q9PRD9" }, "psc": { "uniref90_id": "UniRef90_Q9PRD9", "gene": "RpiB", "product": "Galactoside O-acetyltransferase", "ec_ids": [ "5.3.1.6" ], "uniref50_id": "UniRef50_Q9PRD9", "cog_id": "COG0698", "cog_category": "G", "kegg_orthology_id": "K01808" }, "pscc": { "uniref50_id": "UniRef50_Q9PRD9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PRD9" ], "product": "Galactoside O-acetyltransferase" }, "genes": [ "rpiB" ], "id": "NKIECMJPJD_302", "locus": "GCA000828735_00266" }, { "type": "cds", "sequence": "contig_1", "start": 169829, "stop": 170029, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCAAATAGCCCTTATATACTAAAATTAATTCAAAAGTTAGGTCCGTTATATTGTAGTAGTGCTAATATTTCAGGTGAAGAACCAATTAAAAATCATAATGAAGCAATTTTTAAATTTGGAGCAAATAGTAAACTAATTTATGTTGAAGCACAACAACAAATTGGTGTTCCATCAACTATTGTTGACATCGATAAATGA", "aa": "MPNSPYILKLIQKLGPLYCSSANISGEEPIKNHNEAIFKFGANSKLIYVEAQQQIGVPSTIVDIDK", "aa_hexdigest": "47826b765cb1b8f8bea52daade5f2eb6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7275.315000000001, "isoelectric_point": 6.531050300598144 }, "id": "NKIECMJPJD_303", "locus": "GCA000828735_00267" }, { "type": "cds", "sequence": "contig_1", "start": 170072, "stop": 170302, "strand": "-", "frame": 2, "gene": "tsaC", "product": "L-threonylcarbamoyladenylate synthase", "db_xrefs": [ "COG:COG0009", "COG:J", "SO:0001217", "UniRef:UniRef50_A0A084F1C9", "UniRef:UniRef90_A0AAC9X6K8" ], "nt": "ATGAAAATTTATAGAATAACTAATTTAAATGTGATTTATGATGCTTTAATGGCCAATAAATGTGTTCTAATCCCTACTGATACTATCATTGGTTTATTAGCAAAAAATCAAGATGTAATTTATGAAATTAAGCAACGTGATCGAAATAAGAAAATCATCCGCTTTGTAGCCGATTACAAATTACTTGGTGATTTAACTGTAGAACAAAAGCAATTTTTAGATCTTTTTTGA", "aa": "MKIYRITNLNVIYDALMANKCVLIPTDTIIGLLAKNQDVIYEIKQRDRNKKIIRFVADYKLLGDLTVEQKQFLDLF", "aa_hexdigest": "c60cada1a38a2fb179544b07032b1b4e", "start_type": "ATG", "rbs_motif": null, "genes": [ "tsaC" ], "truncated": "5-prime", "pseudogene": { "start": 169769, "stop": 170302, "observations": { "mutation": [ 169831, 170074 ], "stop": [ 169831, 170074 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAC9X6K8", "query_cov": 1.0, "subject_cov": 0.40860215053763443, "identity": 0.934, "score": 142.0, "evalue": 1.48e-41, "gene_start": 1, "gene_end": 76, "reference_start": 1, "reference_end": 76, "reference_sequence": "MKIYRITNLNAIYDALVANKCVLIPTDTIIGLLAKNQDVIYEIKRRDRNKKIVRFVADYKLLGDLTVEQEQFLDLFWPGSVTVIKNGVSYRMPNSPHILKLIQKLGPLYCSSANISGEEPVKNHNEAIFKFGANSKLIYVEAQQQIGVPSTIVDIDKWEYVRRGANIEMVDMFIKELKYNNTKEKE" }, "paralog": false, "identity": 0.9265536723163842, "score": 332.0, "evalue": 5.09e-119, "description": "Internal stop codon at 169831, 170074. Nonsense mutation around 169831, 170074.", "psc": { "uniref90_id": "UniRef90_A0AAC9X6K8", "gene": "TsaC", "product": "L-threonylcarbamoyladenylate synthase", "uniref50_id": "UniRef50_A0A084F1C9", "cog_id": "COG0009", "cog_category": "J", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAC9X6K8", "UniRef:UniRef50_A0A084F1C9", "COG:COG0009", "COG:J" ] }, "pscc": { "uniref50_id": "UniRef50_A0A084F1C9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A084F1C9" ], "product": "L-threonylcarbamoyladenylate synthase" } }, "id": "NKIECMJPJD_304", "locus": "GCA000828735_00268" }, { "type": "cds", "sequence": "contig_1", "start": 170289, "stop": 171161, "strand": "-", "frame": 1, "gene": "hemK", "product": "peptide chain release factor N(5)-glutamine methyltransferase", "db_xrefs": [ "COG:COG2890", "COG:J", "RefSeq:WP_006688499.1", "SO:0001217", "UniParc:UPI0000165FB4", "UniRef:UniRef100_A0A2C9DXZ5", "UniRef:UniRef50_B5ZAH7", "UniRef:UniRef90_A0A2C9DXZ5" ], "nt": "ATGACCTACCATCAATTAATTTTTCAAGCATACTCACTACTTGAAAAAAAATTACGTAATCCACAAGTAGCATTTCAGTTGCTGTACGGATTAGATAATAAAATTAACGATTCTTATAGTTTTTCAAATAACCGCTTAACAATTGTAAATTCTAATTTAGAATATACATATTTTAAACTATTAGATGAATTTATTAATGGAAAACCATTAGTGAGAATTTTAGGATATGGCTATTTTTGTGCAAAGCGCTTTTATGTTGATAAAAATGTTTTTGCTTTTCGTGTTGAAACTGAATTATTAATAGATGTTGTTAATAAAGTAATTCAACAATCCACTTATAAAATTAAAAATGTTATTGATGTTTGTTGTGGTAGTGGTGTTTTAGGGTTAAGTACAAAAATGAATTTTAATCAATTGAATGTTTCATTATTAGATATTTCTATTGATGCAATTAATAATTGCAAAAAAAATGCTAAATATCATAACTTAACAGATGTAAATTTTATTCATAAAAGTATGCAAGAATACTTTTTAAACACAAAAAAACGGTTTGATTTAATTATCTGTAATCCCCCATATATCAAAAGTAATTATGAATTAAATCGTGAAGTCTTAGATTATGACCCCATTAATGCACTTATTGATTTCGATCACAAAGATGGAATTAGTTTTTATTTATTTATAATAAATAACATTAAATCAATTGCTAATGAGAAATTCACTATTATTTTTGAAATTGGTTTTGATCAAAAAGAAATTTTAGAAAAAGTTATTAAAAAAAATGAATTTCCTCTTTTTTACTACTTTATAAATGATTATAATAATCTATGTAGAATTTTAATAATAAGTAATATTAAATATGAAAATTTATAG", "aa": "MTYHQLIFQAYSLLEKKLRNPQVAFQLLYGLDNKINDSYSFSNNRLTIVNSNLEYTYFKLLDEFINGKPLVRILGYGYFCAKRFYVDKNVFAFRVETELLIDVVNKVIQQSTYKIKNVIDVCCGSGVLGLSTKMNFNQLNVSLLDISIDAINNCKKNAKYHNLTDVNFIHKSMQEYFLNTKKRFDLIICNPPYIKSNYELNREVLDYDPINALIDFDHKDGISFYLFIINNIKSIANEKFTIIFEIGFDQKEILEKVIKKNEFPLFYYFINDYNNLCRILIISNIKYENL", "aa_hexdigest": "958d1714e732d299dbdb8603679da43a", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000165FB4", "ncbi_nrp_id": "WP_006688499.1", "uniref100_id": "UniRef100_A0A2C9DXZ5", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000165FB4", "RefSeq:WP_006688499.1", "UniRef:UniRef100_A0A2C9DXZ5" ] }, "ips": { "uniref100_id": "UniRef100_A0A2C9DXZ5", "uniref90_id": "UniRef90_A0A2C9DXZ5" }, "psc": { "uniref90_id": "UniRef90_A0A2C9DXZ5", "gene": "HemK", "product": "peptide chain release factor N(5)-glutamine methyltransferase", "uniref50_id": "UniRef50_B5ZAH7", "cog_id": "COG2890", "cog_category": "J" }, "pscc": { "uniref50_id": "UniRef50_B5ZAH7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B5ZAH7" ], "product": "peptide chain release factor N(5)-glutamine methyltransferase" }, "genes": [ "hemK" ], "id": "NKIECMJPJD_305", "locus": "GCA000828735_00269" }, { "type": "cds", "sequence": "contig_1", "start": 171401, "stop": 171796, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03462.23", "PFAM:PF00472.26" ], "nt": "ATGAAAATTATAGAAATGACTCCGAATGCTGTTGGTTTTAGTTTTATTTCTTTTATGATTTCTGGAGAAGAAGTTTATTCACGAATGAAATTTGAGTCAGGAGTTCATCGAGTACAACGTGTACCTGCTACTGAATCTAAAGGAAGAGTACATACGTCAACAATTACAGTTGCTGTTCTTCCAGAACAAGATGAAGTTGATGTTGTAATTAATCCAACAGAATTAAGAATTGATACTTACCGTGCAAGTGGGGCTGGAGGACAGCATGTTAATAGAACAGAATCAGCTGTAAGAATTACGCATATTCCAACAGGGGTTGTTGCTGCATGTCAAGAGGGAAAATCACAAATTGAAAACCGAGAAACAGCTATGAAAATGTTAAGAGCTAAATTATGA", "aa": "MKIIEMTPNAVGFSFISFMISGEEVYSRMKFESGVHRVQRVPATESKGRVHTSTITVAVLPEQDEVDVVINPTELRIDTYRASGAGGQHVNRTESAVRITHIPTGVVAACQEGKSQIENRETAMKMLRAKL", "aa_hexdigest": "1a9e1ba3fd7fc1c6b33f27c0ba5afd1b", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI000E5B535F", "query_cov": 0.9083969465648855, "subject_cov": 0.8263888888888888, "identity": 0.58, "score": 150.0, "evalue": 2.46e-44, "valid": false, "product": "PCRF domain-containing protein", "uniref50_id": "UniRef50_A0A7S1SZ85" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03462.23", "name": "PCRF", "length": 57, "aa_cov": 0.42748091603053434, "hmm_cov": 1.0, "evalue": 1.630208371909278e-23, "score": 79.05348205566406, "start": 2, "stop": 57 }, { "id": "PF00472.26", "name": "RF-1", "length": 69, "aa_cov": 0.5190839694656488, "hmm_cov": 1.0, "evalue": 4.104344452026056e-32, "score": 106.15489196777344, "start": 64, "stop": 131 } ], "seq_stats": { "molecular_weight": 14458.414399999989, "isoelectric_point": 7.940973854064941 }, "id": "NKIECMJPJD_306", "locus": "GCA000828735_00270" }, { "type": "cds", "sequence": "contig_1", "start": 171800, "stop": 172240, "strand": "-", "frame": 2, "gene": "prfA", "product": "peptide chain release factor 1", "db_xrefs": [ "COG:COG0216", "COG:J", "GO:0003747", "GO:0005737", "GO:0006415", "GO:0016149", "KEGG:K02835", "SO:0001217", "UniRef:UniRef50_Q9PRE0", "UniRef:UniRef90_Q9PRE0" ], "nt": "ATGGAATACAACAAAAAACTTTATGAAGCAATTGAACGAGTCGCTATTAAAAATGACGCTTTAAAAAAAGAATTAGAAACGGTTGTAACAGACTTTAAAAAAATTAAAGAGATTAATATACAGTTAAAAAAAACTACTAAAATTGCTGAAGCCTTTGCAAAATATAAACAAAAATTGGATACTGGTATAGCTGCTGAAAAAATATTAAATACAGAAAAAGATTTAGAATTAATCGAATTAGCACAGATGGATTTAGATGAAGCTAAAATTAATATTCCAATAATTGAAAATGATTTAAAAATTATGCTGTTGCCAACTGATCCTAATGATGATAAAAATGTTATTGTTGAAATGCGCCCTGCTGCTGGAGGGGATGAATCATCAATTTTTGTCGGCAATTTGTTTGATACATATCGTGCTTATGCTGAAAATAATAATTGA", "aa": "MEYNKKLYEAIERVAIKNDALKKELETVVTDFKKIKEINIQLKKTTKIAEAFAKYKQKLDTGIAAEKILNTEKDLELIELAQMDLDEAKINIPIIENDLKIMLLPTDPNDDKNVIVEMRPAAGGDESSIFVGNLFDTYRAYAENNN", "aa_hexdigest": "0fb5020b617e12e510ea6bb94ec89af5", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "genes": [ "prfA" ], "truncated": "5-prime", "pseudogene": { "start": 171497, "stop": 172240, "observations": { "mutation": [ 171802 ], "stop": [ 171802 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PRE0", "query_cov": 1.0, "subject_cov": 0.40668523676880225, "identity": 1.0, "score": 272.0, "evalue": 2.81e-89, "gene_start": 1, "gene_end": 146, "reference_start": 1, "reference_end": 146, "reference_sequence": "MEYNKKLYEAIERVAIKNDALKKELETVVTDFKKIKEINIQLKKTTKIAEAFAKYKQKLDTGIAAEKILNTEKDLELIELAQMDLDEAKINIPIIENDLKIMLLPTDPNDDKNVIVEMRPAAGGDESSIFVGNLFDTYRAYAENNNWKMKIIEMTPNAVGFSFISFMISGEEVYSRMKFESGVHRVQRVPATESKGRVHTSTITVAVLPEQDEVDVVINPTELRIDTYRASGAGGQHVNRTESAVRITHIPTGVVAACQEGKSQIENRETAMKMLRAKLWEAAQEQQNAEFANLRKNQVGTGDRSEKIRTYNYPQNRVTDHRINLTLNKLDQIMMGELDEIIDALIADEQTGLMANLDI" }, "paralog": false, "identity": 0.9959514170040485, "score": 476.0, "evalue": 8.84e-172, "description": "Internal stop codon at 171802. Nonsense mutation around 171802.", "psc": { "uniref90_id": "UniRef90_Q9PRE0", "gene": "prfA", "product": "peptide chain release factor 1", "uniref50_id": "UniRef50_Q9PRE0", "cog_id": "COG0216", "cog_category": "J", "kegg_orthology_id": "K02835", "go_ids": [ "GO:0003747", "GO:0005737", "GO:0006415", "GO:0016149" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PRE0", "UniRef:UniRef50_Q9PRE0", "COG:COG0216", "COG:J", "KEGG:K02835", "GO:0003747", "GO:0005737", "GO:0006415", "GO:0016149" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PRE0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PRE0" ], "product": "Peptide chain release factor 1" } }, "id": "NKIECMJPJD_307", "locus": "GCA000828735_00271" }, { "type": "cds", "sequence": "contig_1", "start": 172373, "stop": 172660, "strand": "-", "frame": 2, "gene": "rpmE", "product": "50S ribosomal protein L31", "db_xrefs": [ "COG:COG0254", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904", "RefSeq:WP_006688758.1", "SO:0001217", "UniParc:UPI0000133F35", "UniRef:UniRef100_Q9PRE1", "UniRef:UniRef50_Q9PRE1", "UniRef:UniRef90_Q9PRE1" ], "nt": "ATGAGAGATATTCATCCAGTAAGCAAACCATGCGTTTATAATTGTGTAACATGTAAAAAAGAATTTATAATTGATTCTGCAGCAAAAAATACAGAAGTTGCAATTGAAGTTTGTTCAAATTGCCATACATTTTTTATTGGTAAACAAAACGCAACTACAACATTACGTGGTCGTGCTGAAAAACTAAATAATCGTTTTGAAGCTGGTTTAAATAACATTAATAAAAAACCAGAAAAGAAAAAAATACAAGGTAAATCTGAACCAAGAAAAAGTTTAAACGAATTATAA", "aa": "MRDIHPVSKPCVYNCVTCKKEFIIDSAAKNTEVAIEVCSNCHTFFIGKQNATTTLRGRAEKLNNRFEAGLNNINKKPEKKKIQGKSEPRKSLNEL", "aa_hexdigest": "17dbc090192fce23910a07d4469078fb", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0000133F35", "ncbi_nrp_id": "WP_006688758.1", "uniref100_id": "UniRef100_Q9PRE1", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000133F35", "RefSeq:WP_006688758.1", "UniRef:UniRef100_Q9PRE1" ] }, "ips": { "uniref100_id": "UniRef100_Q9PRE1", "uniref90_id": "UniRef90_Q9PRE1" }, "psc": { "uniref90_id": "UniRef90_Q9PRE1", "gene": "rpmE", "product": "50S ribosomal protein L31", "uniref50_id": "UniRef50_Q9PRE1", "cog_id": "COG0254", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PRE1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PRE1" ], "product": "Large ribosomal subunit protein bL31" }, "genes": [ "rpmE" ], "id": "NKIECMJPJD_308", "locus": "GCA000828735_00272" }, { "type": "cds", "sequence": "contig_1", "start": 173185, "stop": 174174, "strand": "-", "frame": 3, "gene": "dnaA", "product": "chromosomal replication initiator protein DnaA", "db_xrefs": [ "COG:COG0593", "COG:L", "GO:0003677", "GO:0003688", "GO:0005524", "GO:0005737", "GO:0005886", "GO:0006270", "GO:0006275", "KEGG:K02313", "SO:0001217", "UniRef:UniRef50_Q9PRE2", "UniRef:UniRef90_Q9PRE2" ], "nt": "ATGGCTAATAATTATCAAACTTTATATGATTCAGCAATAAAAAGGATTCCATACGATCTTATTTCTGATCAAGCTTATGCAATTCTACAAAATGCTAAAACTCATAAAGTTTGCGATGGTGTTTTATATATAATTGTAGCCAATGCCTTTGAAAAAAGTATTATTAACGGTAATTTTATTAACATTATTTCTAAATATCTAAGCGAAGAATTCAAAAAGGAAAATATTGTTAATTTTGAATTTATTATAGACAATGAAAAATTATTAATTAATAGCAATTTTTTAATTAAAGAAACTAATATTAAAAATCGTTTTAATTTTAGTGATGAACTTTTACGTTACAATTTTAACAATTTAGTAATTAGTAATTTTAATCAAAAAGCGATTAAGGCGATTGAAAATTTATTTTCAAATAACTATGATAATAGTTCAATGTGTAACCCTTTATTTTTATTTGGTAAAGTTGGTGTTGGTAAAACGCATATCGTGGCTGCTGCTGGTAATCGTTTTGCTAATAGTAATCCTAATTTAAAAATTTATTATTATGAAGGGCAAGATTTTTTTCGAAAGTTTTGTTCTGCTTCGTTAAAAGGGACTAGTTATGTTGAAGAGTTTAAAAAAGAAATTGCTTCAGCAGATTTATTAATTTTTGAAGATATTCAAAATATCCAATCACGTGATTCAACGGCTGAATTGTTTTTTAATATCTTTAATGATATAAAATTAAATGGTGGAAAAATTATCTTAACATCTGACCGTACACCAAACGAACTTAATGGTTTTCATAATCGAATTATTTCGAGATTAGCGTCAGGTTTGCAGTGTAAAATTTCTCAACCCGACAAAAATGAAGCTATTAAAATTATTAATAATTGGTTTGAATTCAAAAAAAAATATCAAATTACTGACGAAGCTAAAGAATATATTGCTGAAGGTTTTCACACTGATATTAGACAGATGATTGGTAATCTAAAACAAATTTGTTTTTGA", "aa": "MANNYQTLYDSAIKRIPYDLISDQAYAILQNAKTHKVCDGVLYIIVANAFEKSIINGNFINIISKYLSEEFKKENIVNFEFIIDNEKLLINSNFLIKETNIKNRFNFSDELLRYNFNNLVISNFNQKAIKAIENLFSNNYDNSSMCNPLFLFGKVGVGKTHIVAAAGNRFANSNPNLKIYYYEGQDFFRKFCSASLKGTSYVEEFKKEIASADLLIFEDIQNIQSRDSTAELFFNIFNDIKLNGGKIILTSDRTPNELNGFHNRIISRLASGLQCKISQPDKNEAIKIINNWFEFKKKYQITDEAKEYIAEGFHTDIRQMIGNLKQICF", "aa_hexdigest": "37fdf62d77110ca8a53eab2cced72a7e", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "genes": [ "dnaA" ], "truncated": "5-prime", "pseudogene": { "start": 172882, "stop": 174174, "observations": { "mutation": [ 172989, 173187 ], "stop": [ 172989, 173187 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PRE2", "query_cov": 1.0, "subject_cov": 0.7199124726477024, "identity": 1.0, "score": 650.0, "evalue": 9.76e-234, "gene_start": 1, "gene_end": 329, "reference_start": 1, "reference_end": 329, "reference_sequence": "MANNYQTLYDSAIKRIPYDLISDQAYAILQNAKTHKVCDGVLYIIVANAFEKSIINGNFINIISKYLSEEFKKENIVNFEFIIDNEKLLINSNFLIKETNIKNRFNFSDELLRYNFNNLVISNFNQKAIKAIENLFSNNYDNSSMCNPLFLFGKVGVGKTHIVAAAGNRFANSNPNLKIYYYEGQDFFRKFCSASLKGTSYVEEFKKEIASADLLIFEDIQNIQSRDSTAELFFNIFNDIKLNGGKIILTSDRTPNELNGFHNRIISRLASGLQCKISQPDKNEAIKIINNWFEFKKKYQITDEAKEYIAEGFHTDIRQMIGNLKQICFWADNDTNKDLIITKDYVIECSVENEIPLNIVVKKQFKPEQIIEIVAKELNIKTDLIKSSIRKNNIVWARDIVCYILKNKLNLTLTDIGKLLNGREHTTISHSISKVQKILDDENSQEALQINLIINKF" }, "paralog": false, "identity": 0.9953488372093023, "score": 847.0, "evalue": 5.39e-314, "description": "Internal stop codon at 172989, 173187. Nonsense mutation around 172989, 173187.", "psc": { "uniref90_id": "UniRef90_Q9PRE2", "gene": "dnaA", "product": "chromosomal replication initiator protein DnaA", "uniref50_id": "UniRef50_Q9PRE2", "cog_id": "COG0593", "cog_category": "L", "kegg_orthology_id": "K02313", "go_ids": [ "GO:0003677", "GO:0003688", "GO:0005524", "GO:0005737", "GO:0005886", "GO:0006270", "GO:0006275" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PRE2", "UniRef:UniRef50_Q9PRE2", "COG:COG0593", "COG:L", "KEGG:K02313", "GO:0003677", "GO:0003688", "GO:0005524", "GO:0005737", "GO:0005886", "GO:0006270", "GO:0006275" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PRE2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PRE2" ], "product": "Chromosomal replication initiator protein DnaA" } }, "id": "NKIECMJPJD_309", "locus": "GCA000828735_00273" }, { "type": "cds", "sequence": "contig_1", "start": 174345, "stop": 174491, "strand": "+", "frame": 3, "gene": "rpmH", "product": "50S ribosomal protein L34", "db_xrefs": [ "COG:COG0230", "COG:J", "GO:0000315", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625", "GO:1990904", "RefSeq:WP_004026378.1", "SO:0001217", "UniParc:UPI0000133FDD", "UniRef:UniRef100_Q9PPN5", "UniRef:UniRef50_Q8EU89", "UniRef:UniRef90_Q9PPN5" ], "nt": "ATGAAAAGAACGTTTCAACCTAATAATCGTAAAAGAGCAAAAGTGCATGGTTTTCGTGCACGCATGAAAACAAAAAATGGGCGTAATGTTTTAGCTAGAAGAAGACTTAAAGGGCGTCATAGTCTAACAGTTTCTAGTGAAAAATAA", "aa": "MKRTFQPNNRKRAKVHGFRARMKTKNGRNVLARRRLKGRHSLTVSSEK", "aa_hexdigest": "2dfe555e2e7567f932d329b6ccd7168e", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "ups": { "uniparc_id": "UPI0000133FDD", "ncbi_nrp_id": "WP_004026378.1", "uniref100_id": "UniRef100_Q9PPN5", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000133FDD", "RefSeq:WP_004026378.1", "UniRef:UniRef100_Q9PPN5" ] }, "ips": { "uniref100_id": "UniRef100_Q9PPN5", "uniref90_id": "UniRef90_Q9PPN5" }, "psc": { "uniref90_id": "UniRef90_Q9PPN5", "gene": "rpmH", "product": "50S ribosomal protein L34", "uniref50_id": "UniRef50_Q8EU89", "cog_id": "COG0230", "cog_category": "J", "go_ids": [ "GO:0000315", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_Q8EU89", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q8EU89" ], "product": "Large ribosomal subunit protein bL34" }, "genes": [ "rpmH" ], "id": "NKIECMJPJD_310", "locus": "GCA000828735_00274" }, { "type": "cds", "sequence": "contig_1", "start": 174497, "stop": 174838, "strand": "+", "frame": 2, "gene": "rnpA", "product": "ribonuclease P protein component", "db_xrefs": [ "COG:COG0594", "COG:J", "EC:3.1.26.5", "GO:0000049", "GO:0001682", "GO:0004526", "GO:0008033", "GO:0030677", "GO:0042781", "RefSeq:WP_006688777.1", "SO:0001217", "UniParc:UPI0000134448", "UniRef:UniRef100_Q9PPN6", "UniRef:UniRef50_Q9PPN6", "UniRef:UniRef90_Q9PPN6" ], "nt": "ATGGCTAATTTTATTAGTCTAAAAAAAAATGAAGATATACTTGATACAATTAAGAAACAACAAAAAATTCATTCTAATCAAATCGTAGTTTATTTTCGTAAAACCAATCTAAAAAATGTTCGCTTAGCTATAAGCATTTCTAAAAAAAAGTTTAAATTAGCAACACAACGCAATCGAATTCGACGCCTGATTAAAGCATGGTTTATTGCTGCTGATATTCCCATTAAAAGTTATGATATTGTAGTCTTAGTGAAACCATCGTTTATTGATGGTTCTTTTGTTTTAAATTGTAATAATCTTAAAATAATTCTTCAACGAATTATTAACAAAGAAAAAAGATAA", "aa": "MANFISLKKNEDILDTIKKQQKIHSNQIVVYFRKTNLKNVRLAISISKKKFKLATQRNRIRRLIKAWFIAADIPIKSYDIVVLVKPSFIDGSFVLNCNNLKIILQRIINKEKR", "aa_hexdigest": "cd69982e3371440b5688026fbe3951f8", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000134448", "ncbi_nrp_id": "WP_006688777.1", "uniref100_id": "UniRef100_Q9PPN6", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000134448", "RefSeq:WP_006688777.1", "UniRef:UniRef100_Q9PPN6" ] }, "ips": { "uniref100_id": "UniRef100_Q9PPN6", "uniref90_id": "UniRef90_Q9PPN6" }, "psc": { "uniref90_id": "UniRef90_Q9PPN6", "gene": "rnpA", "product": "ribonuclease P protein component", "ec_ids": [ "3.1.26.5" ], "uniref50_id": "UniRef50_Q9PPN6", "cog_id": "COG0594", "cog_category": "J", "go_ids": [ "GO:0000049", "GO:0001682", "GO:0004526", "GO:0008033", "GO:0030677", "GO:0042781" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PPN6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PPN6" ], "product": "Ribonuclease P protein component" }, "genes": [ "rnpA" ], "id": "NKIECMJPJD_311", "locus": "GCA000828735_00275" }, { "type": "cds", "sequence": "contig_1", "start": 174877, "stop": 175083, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCATCTGTTGATAAACAAAATTTAACAAATAGAATGCGGATTTCTGTTTCACATTTTGCTGGAGCATCGAATGCAAATTCGATAAAAAAAGAACGTAAGAAAAAAATTTTTAATGTTTTATTAAAAGTTTTTAAAATCATCGTTTATGCATTTTTCTTAGGTATTGGCTTATATGGTTGTTTCCAAAATATGGCAAACCACTGA", "aa": "MSSVDKQNLTNRMRISVSHFAGASNANSIKKERKKKIFNVLLKVFKIIVYAFFLGIGLYGCFQNMANH", "aa_hexdigest": "100c5208ba58503446b82b1a41071a70", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7739.078499999997, "isoelectric_point": 10.362728691101072 }, "id": "NKIECMJPJD_312", "locus": "GCA000828735_00276" }, { "type": "cds", "sequence": "contig_1", "start": 175204, "stop": 175302, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCTGATTTTTCATTTGATTATGGTCCTTTCTATGCTTTATTTGTTTGGCCAATTGCACAAATTTTATTACATTTTATGTATGCAACGAGAGATTGA", "aa": "MSDFSFDYGPFYALFVWPIAQILLHFMYATRD", "aa_hexdigest": "464809b5a274e051a18d1194fe650034", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3863.4151, "isoelectric_point": 4.647690391540528 }, "id": "NKIECMJPJD_313", "locus": "GCA000828735_00277" }, { "type": "cds", "sequence": "contig_1", "start": 175366, "stop": 175638, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGATCTCAGCACGTGCAACTATCCAAACAGAAAGAATTTCTGAAATTCAAGGAAAAATTGCAGAAATTAATGCAAAATATAAAGACGCAAAAGATATGCAGTCTCGTCAAAAAAAACAAATGGAAACAAAAGAGTTATATCAAAAACATAACGTTAAACCTTTAGCTCCTTTTGAAAGTATGATTATTACATTGCCGATCTTTTTAATTATTTATCGTGTTGTTACAATTTTAAGACCACTTAAGTTTATTAGTATTTTTTATATTTGA", "aa": "MLISARATIQTERISEIQGKIAEINAKYKDAKDMQSRQKKQMETKELYQKHNVKPLAPFESMIITLPIFLIIYRVVTILRPLKFISIFYI", "aa_hexdigest": "56212c81309cbf1a998a80ca3fd18d64", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10585.562999999991, "isoelectric_point": 9.87057704925537 }, "id": "NKIECMJPJD_314", "locus": "GCA000828735_00278" }, { "type": "cds", "sequence": "contig_1", "start": 176030, "stop": 176191, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00398.26" ], "nt": "TTGAATAAATCATTCATTAAAAATAAACTCAAGCAAGAAAGTTTTGTTCCATCAAAAAAAATGGGGCAAAACTTCTTATTGTCTAATAACATAAAAAATAAAATTGTAGATGTGGCGAATATTAATAAAGATGATCTTATATTAGAAATTGGTCCAGGGTGA", "aa": "MNKSFIKNKLKQESFVPSKKMGQNFLLSNNIKNKIVDVANINKDDLILEIGPG", "aa_hexdigest": "06a7f9ce0264fe3f07b891cada4ddd8c", "start_type": "TTG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00398.26", "name": "RrnaAD", "length": 35, "aa_cov": 0.660377358490566, "hmm_cov": 1.0, "evalue": 1.0711867595590405e-09, "score": 33.356536865234375, "start": 19, "stop": 53 } ], "seq_stats": { "molecular_weight": 5974.9468000000015, "isoelectric_point": 9.69941349029541 }, "id": "NKIECMJPJD_315", "locus": "GCA000828735_00279" }, { "type": "cds", "sequence": "contig_1", "start": 176342, "stop": 176863, "strand": "+", "frame": 2, "gene": "rsmA", "product": "16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase RsmA", "db_xrefs": [ "EC:2.1.1.182", "GO:0000179", "GO:0006364", "SO:0001217", "UniRef:UniRef50_Q9PPN8", "UniRef:UniRef90_UPI001152E4FE" ], "nt": "TTGATTTTAGATTACAATAATACTCAAAAAATTCAAAAAATTAAAGTTGTTGCAAATCTTCCATATGCAATATCTTCAAAGATAGTTTTAAAAATTATTCAATCAAAATTAATTAATGATGCTTATATTATGGTTCAAAAAGAAATGGCTGAACGAATTGGTGCTAAAGTAAATACACGTGGTTATAATGCTTTTACTGTTTTAGTACAATTATTCTGTAAAACAAAAATTCTATTCGAAGTTAATGCTAAAGAATTTCATCCCCAACCTAAAGTTCAAAGTGCTGTCATTCATCTTGAAAATCTTCATAATAGTGTGAATTTTAATATTGAAGAATTAGGTAAATTTTTACGGATTTGTTTTTTAAATAAACGAAAAAAGTTAAAAAATAACTTATCTAATATTTATGATATTAAAATAATTAATCAAATGTTTATTGATTATAATTTAGATATGAATTTACGAGCTGAAAACATTGAACCAAAAATGTTTCTAAAACTCTTTAATTATTTGAATAGATAA", "aa": "MILDYNNTQKIQKIKVVANLPYAISSKIVLKIIQSKLINDAYIMVQKEMAERIGAKVNTRGYNAFTVLVQLFCKTKILFEVNAKEFHPQPKVQSAVIHLENLHNSVNFNIEELGKFLRICFLNKRKKLKNNLSNIYDIKIINQMFIDYNLDMNLRAENIEPKMFLKLFNYLNR", "aa_hexdigest": "14c8706d1ddfbaa9d5188368a92fd627", "start_type": "TTG", "rbs_motif": null, "genes": [ "rsmA" ], "truncated": "5-prime", "pseudogene": { "start": 176042, "stop": 176863, "observations": { "mutation": [ 176189 ], "stop": [ 176189 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001152E4FE", "query_cov": 1.0, "subject_cov": 0.6245487364620939, "identity": 0.9940000000000001, "score": 337.0, "evalue": 1.21e-115, "gene_start": 1, "gene_end": 173, "reference_start": 105, "reference_end": 277, "reference_sequence": "MNKSFIKNKLKQESFVPSKKMGQNFLLSNNIKNKIVDVANINKDDLILEIGPGWGAITEILVQKTNILIAIELDKRLYAHLKTYIKTSNFNIINNDVLCVDLDNLILDYNNTQKIQKIKVVANLPYAISSKIVLKIIQSKLINDAYIMVQKEMAERIGAKVNTRGYNAFTVLVQLFCKTKILFEVNAKEFHPQPKVQSAVIHLENLHNSVNFNIEELGKFLRICFLNKRKKLKNNLSNIYDIKIINQMFIDYNLDMNLRAENIEPKMFLKLFNYLNR" }, "paralog": false, "identity": 0.9926739926739927, "score": 530.0, "evalue": 3.15e-194, "description": "Internal stop codon at 176189. Nonsense mutation around 176189.", "psc": { "uniref90_id": "UniRef90_UPI001152E4FE", "gene": "rsmA", "product": "16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase RsmA", "ec_ids": [ "2.1.1.182" ], "uniref50_id": "UniRef50_Q9PPN8", "go_ids": [ "GO:0000179", "GO:0006364" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001152E4FE", "EC:2.1.1.182", "UniRef:UniRef50_Q9PPN8", "GO:0000179", "GO:0006364" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PPN8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PPN8" ], "product": "Ribosomal RNA small subunit methyltransferase A" } }, "id": "NKIECMJPJD_316", "locus": "GCA000828735_00280" }, { "type": "cds", "sequence": "contig_1", "start": 176897, "stop": 177463, "strand": "+", "frame": 2, "gene": "ispE", "product": "4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase", "db_xrefs": [ "COG:COG1947", "COG:I", "EC:2.7.1.148", "GO:0005524", "GO:0016114", "GO:0019288", "GO:0050515", "KEGG:K00919", "SO:0001217", "UniRef:UniRef50_Q9PPN9", "UniRef:UniRef90_Q9PPN9" ], "nt": "ATGAAAGTAAAGTCATTCGCAAAAATTGATTTAGGATATAGTGTTTATAAAAAACAAAAGAATTTTACAAAACACGATTTTGAATCAATTTTTATACTTGTTGAAAATATTTATGACGATATTGAAATTACTAAAATAGAAAAAAATATTGATGATGTTCATTACTACAATGAAACCAATGAAATTTATGTTTATAGCCGTTTAGTTCATAAGACATTAGAATGGATTCGTCATACTTATCATATTAAAAATCATTATCGAATTAATATTAAAAAAAGAATTCCAATTGGTGCAGGCTTAGGTGGTGGTTCATCAAACGCTGCAGCCATTATGAAATATATTCTAGAATTTGAGGGTATTAAAGAAATTAATTATAAAGATGTTGTTAATAAATTAGGTGCTGATATTCCCTTTTTTTTATCAGGCTATAAAACAGCTTATATAAGTGATTACGGAAGTGTTTTAGAGGATTTGACTGGTCAATTTAAATTAAATTATGAAGTTTATTTAATGAATGTTAATGTAAATACAAAAATTGTTTTTGAAAAATTTGATGATAATTCATGA", "aa": "MKVKSFAKIDLGYSVYKKQKNFTKHDFESIFILVENIYDDIEITKIEKNIDDVHYYNETNEIYVYSRLVHKTLEWIRHTYHIKNHYRINIKKRIPIGAGLGGGSSNAAAIMKYILEFEGIKEINYKDVVNKLGADIPFFLSGYKTAYISDYGSVLEDLTGQFKLNYEVYLMNVNVNTKIVFEKFDDNS", "aa_hexdigest": "fb3a3ad02f5ee6e62aead7acc36ccaa9", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "ispE" ], "truncated": "3-prime", "pseudogene": { "start": 176897, "stop": 177694, "observations": { "mutation": [ 177461 ], "stop": [ 177461 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PPN9", "query_cov": 1.0, "subject_cov": 0.7094339622641509, "identity": 1.0, "score": 377.0, "evalue": 4.32e-131, "gene_start": 1, "gene_end": 188, "reference_start": 1, "reference_end": 188, "reference_sequence": "MKVKSFAKIDLGYSVYKKQKNFTKHDFESIFILVENIYDDIEITKIEKNIDDVHYYNETNEIYVYSRLVHKTLEWIRHTYHIKNHYRINIKKRIPIGAGLGGGSSNAAAIMKYILEFEGIKEINYKDVVNKLGADIPFFLSGYKTAYISDYGSVLEDLTGQFKLNYEVYLMNVNVNTKIVFEKFDDNSWHVIKNNFKTIIKNLKENIVVNIHNDLQEYCFELYPNIKYKYNELLSDGFYTILSGAGSSFIRIKLKNKEDLIINEN" }, "paralog": false, "identity": 0.9962264150943396, "score": 534.0, "evalue": 1.08e-195, "description": "Internal stop codon at 177461. Nonsense mutation around 177461.", "psc": { "uniref90_id": "UniRef90_Q9PPN9", "gene": "ispE", "product": "4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase", "ec_ids": [ "2.7.1.148" ], "uniref50_id": "UniRef50_Q9PPN9", "cog_id": "COG1947", "cog_category": "I", "kegg_orthology_id": "K00919", "go_ids": [ "GO:0050515", "GO:0005524", "GO:0019288", "GO:0016114" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PPN9", "EC:2.7.1.148", "UniRef:UniRef50_Q9PPN9", "COG:COG1947", "COG:I", "KEGG:K00919", "GO:0050515", "GO:0005524", "GO:0019288", "GO:0016114" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PPN9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PPN9" ], "product": "4-diphosphocytidyl-2-C-methyl-D-erythritol kinase" } }, "id": "NKIECMJPJD_317", "locus": "GCA000828735_00281" }, { "type": "cds", "sequence": "contig_1", "start": 177684, "stop": 177872, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00749.27" ], "nt": "ATGAAAATTAGAACACGTTATGCTCCATCACCAACCGGTTATTTACATATTGGAGGAGCGCGAACAGCTTTATTTAATTATTTACTAGCAAAAGCTTATGGTGGGGATTTTATTATTCGTATTGAAGATACTGATATTGAAAGAAATGTGGAAGGGGGAATTAATTCCCAACTAGATTTTTTAGCATGA", "aa": "MKIRTRYAPSPTGYLHIGGARTALFNYLLAKAYGGDFIIRIEDTDIERNVEGGINSQLDFLA", "aa_hexdigest": "44203cadf53c21804e2d95111d2f1714", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "psc": { "uniref90_id": "UniRef90_UPI0016AA1FE4", "query_cov": 0.967741935483871, "subject_cov": 0.8333333333333334, "identity": 0.6829999999999999, "score": 85.1, "evalue": 1.46e-20, "valid": false, "product": "glutamate--tRNA ligase family protein", "uniref50_id": "UniRef50_A0A5J4P5N7" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00749.27", "name": "tRNA-synt_1c", "length": 60, "aa_cov": 0.967741935483871, "hmm_cov": 1.0, "evalue": 1.671119435734857e-29, "score": 98.28517150878906, "start": 2, "stop": 61 } ], "seq_stats": { "molecular_weight": 6904.773399999999, "isoelectric_point": 6.534062767028809 }, "id": "NKIECMJPJD_318", "locus": "GCA000828735_00282" }, { "type": "cds", "sequence": "contig_1", "start": 177873, "stop": 178541, "strand": "+", "frame": 3, "gene": "gltX", "product": "glutamate--tRNA ligase", "db_xrefs": [ "COG:COG0008", "COG:J", "EC:6.1.1.17", "GO:0000049", "GO:0000166", "GO:0004818", "GO:0005524", "GO:0005829", "GO:0006424", "GO:0008270", "SO:0001217", "UniRef:UniRef50_P47700", "UniRef:UniRef90_Q9PPP0" ], "nt": "ATGGGTATTATTCCAGACGAATCAATTCGTAATCCTAAAGCTTTTGGCCCATATATCCAATCTGAAAAATTAAAACATTATGAAAAATTAGCATTAGATCTAGTTGATCAAAAAAAAGCTTATTTTTGTTTTTGTAGTAAAGAACAATTAGATGCAGATCGCGAATTAGCTGAAAAATCACATCAAACACCTAAATACAAACGGCACTGTTTAAATTTAGATAAAAAAACAATTGAAAGTAACTTATTACAAAATAAAGAATACACAATTCGTTTAAAAATTAATGAAAATATGGAATATAGCTGGGATGATTTAATTCGGGGTAAAATTAGTATTCCAGGTAGTGCTTTAACTGATCCTGTCATTTTAAAATCAAATAAGATTGCAATGTATAATTTTGCAGTTGTAATTGACGATTATGAAATGCAAATTTCACATGTCATTCGTGGAGAAGAGCATATTTCTAATACACCTTATCAATTAGCGATTGCTCAGGCCTTAAATTATGATATAACAAAAATTAAATATGGTCATTTGTCAATTATTGTTGATGAGACAGGAAAAAAATTATCAAAGCGAAATTTATCTTTAAAACAGTTTGTGTCTGATTATGAAAAAGATGGTTATTGGCCACATGCTATTACAAACTTTGTTGCATTATTAGGTTGA", "aa": "MGIIPDESIRNPKAFGPYIQSEKLKHYEKLALDLVDQKKAYFCFCSKEQLDADRELAEKSHQTPKYKRHCLNLDKKTIESNLLQNKEYTIRLKINENMEYSWDDLIRGKISIPGSALTDPVILKSNKIAMYNFAVVIDDYEMQISHVIRGEEHISNTPYQLAIAQALNYDITKIKYGHLSIIVDETGKKLSKRNLSLKQFVSDYEKDGYWPHAITNFVALLG", "aa_hexdigest": "f886159273085b45536e7b62cf73271b", "start_type": "ATG", "rbs_motif": null, "genes": [ "gltX" ], "truncated": "both", "pseudogene": { "start": 177684, "stop": 178844, "observations": { "mutation": [ 177870, 178539 ], "stop": [ 177870, 178539 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PPP0", "query_cov": 1.0, "subject_cov": 0.4605809128630705, "identity": 1.0, "score": 446.0, "evalue": 8.84e-155, "gene_start": 1, "gene_end": 222, "reference_start": 64, "reference_end": 285, "reference_sequence": "MKIRTRYAPSPTGYLHIGGARTALFNYLLAKAYGGDFIIRIEDTDIERNVEGGINSQLDFLAWMGIIPDESIRNPKAFGPYIQSEKLKHYEKLALDLVDQKKAYFCFCSKEQLDADRELAEKSHQTPKYKRHCLNLDKKTIESNLLQNKEYTIRLKINENMEYSWDDLIRGKISIPGSALTDPVILKSNKIAMYNFAVVIDDYEMQISHVIRGEEHISNTPYQLAIAQALNYDITKIKYGHLSIIVDETGKKLSKRNLSLKQFVSDYEKDGYWPHAITNFVALLGWSPKNNDEIMSLETMIKNFDINNLSKSPAFFDINKMNWFSTQYFNNITQEEFINFIKKHSLTKELVLNDYTFINKCLLFKSHIINLKQLIDLVIEQFNCDKKVLASDVDYIKKNQLITVVRVFYEQLIINDEFNEEFIKEIIKKVQIITNNKGANLYMPIRIATTFSSHGPELAKTICYLGREKVLKNLINILKILD" }, "paralog": false, "identity": 0.9948186528497409, "score": 773.0, "evalue": 3.47e-286, "description": "Internal stop codon at 177870, 178539. Nonsense mutation around 177870, 178539.", "psc": { "uniref90_id": "UniRef90_Q9PPP0", "gene": "gltX", "product": "glutamate--tRNA ligase", "ec_ids": [ "6.1.1.17" ], "uniref50_id": "UniRef50_P47700", "cog_id": "COG0008", "cog_category": "J", "go_ids": [ "GO:0000049", "GO:0000166", "GO:0004818", "GO:0005524", "GO:0005829", "GO:0006424", "GO:0008270" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PPP0", "EC:6.1.1.17", "UniRef:UniRef50_P47700", "COG:COG0008", "COG:J", "GO:0000049", "GO:0000166", "GO:0004818", "GO:0005524", "GO:0005829", "GO:0006424", "GO:0008270" ] }, "pscc": { "uniref50_id": "UniRef50_P47700", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47700" ], "product": "Glutamate--tRNA ligase" } }, "id": "NKIECMJPJD_319", "locus": "GCA000828735_00283" }, { "type": "cds", "sequence": "contig_1", "start": 178581, "stop": 179132, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF19269.4" ], "nt": "ATGATTAAAAATTTTGATATCAATAATTTATCAAAATCCCCCGCCTTTTTTGATATCAATAAAATGAATTGGTTTTCAACACAATATTTTAATAATATTACCCAAGAAGAATTTATCAATTTTATAAAGAAACATTCATTAACTAAAGAATTAGTTTTGAATGATTATACTTTTATTAATAAATGTCTATTATTTAAATCTCATATTATTAATTTAAAACAACTAATTGATTTAGTAATTGAACAATTTAATTGTGATAAAAAAGTATTAGCATCAGATGTAGATTATATTAAAAAAAACCAATTAATAACTGTTGTTCGTGTTTTTTATGAACAATTAATAATTAATGATGAATTTAATGAAGAATTTATAAAAGAAATAATTAAAAAAGTTCAAATAATAACTAATAACAAAGGTGCTAATTTATACATGCCAATTCGTATTGCAACTACATTTAGTTCGCATGGTCCAGAATTAGCTAAAACAATTTGTTATTTAGGACGCGAAAAAGTATTAAAAAATTTAATAAATATTTTAAAAATTTTGGATTAA", "aa": "MIKNFDINNLSKSPAFFDINKMNWFSTQYFNNITQEEFINFIKKHSLTKELVLNDYTFINKCLLFKSHIINLKQLIDLVIEQFNCDKKVLASDVDYIKKNQLITVVRVFYEQLIINDEFNEEFIKEIIKKVQIITNNKGANLYMPIRIATTFSSHGPELAKTICYLGREKVLKNLINILKILD", "aa_hexdigest": "85bb9578639dac22ae07c288b0660363", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF19269.4", "name": "Anticodon_2", "length": 118, "aa_cov": 0.6229508196721312, "hmm_cov": 1.0, "evalue": 1.3854438181945573e-12, "score": 43.794464111328125, "start": 62, "stop": 175 } ], "seq_stats": { "molecular_weight": 21562.018100000023, "isoelectric_point": 8.847850608825684 }, "id": "NKIECMJPJD_320", "locus": "GCA000828735_00284" }, { "type": "cds", "sequence": "contig_1", "start": 179507, "stop": 179614, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTATTACTAATCCTAAAAGTGAAATTAATAAAATTTTAATTAAACATAATATCAATCCAGTTGATGTTGCTAATATAATTGATCATAAATCCAAAAAAATGTGA", "aa": "MIITNPKSEINKILIKHNINPVDVANIIDHKSKKM", "aa_hexdigest": "dde3bb1c436df29535245facd69c9010", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4010.7687000000005, "isoelectric_point": 9.699735832214355 }, "id": "NKIECMJPJD_321", "locus": "GCA000828735_00285" }, { "type": "cds", "sequence": "contig_1", "start": 179615, "stop": 179776, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAACAGGTTATTTCATCTCAAATTGATGCAGATCGTTTAGATTATTTGCCACGTGATTCTCATTTTACAGGAACCAAGTATGGAAATATAGACTATGATAATATCTTTAAAAAGGCAATAGTTTTTAACGATCAATTATGTTTTTCAAAACACTCTTGA", "aa": "MQQVISSQIDADRLDYLPRDSHFTGTKYGNIDYDNIFKKAIVFNDQLCFSKHS", "aa_hexdigest": "683adfa00052afb65cfa7f22b9a55e5f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6184.835300000001, "isoelectric_point": 5.977495384216309 }, "id": "NKIECMJPJD_322", "locus": "GCA000828735_00286" }, { "type": "cds", "sequence": "contig_1", "start": 179813, "stop": 179959, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTGAAAATGTTTATTTAAATAAAACACCAACAACCTATGATATTTTAATGAAAAAGACTTTAGCAAGAGTGCATTTTTTATACCAAAAACAAAAAAAATATATATTCAAAAATTCACAATTATTAACAATTTTAAAACCATGA", "aa": "MFENVYLNKTPTTYDILMKKTLARVHFLYQKQKKYIFKNSQLLTILKP", "aa_hexdigest": "53dd1193d445544611a7d0c8cc5dbcfa", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5851.964800000001, "isoelectric_point": 9.998740196228027 }, "id": "NKIECMJPJD_323", "locus": "GCA000828735_00287" }, { "type": "cds", "sequence": "contig_1", "start": 180026, "stop": 180331, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCGATCGTTTGCTAATGAACATGATTTTATTTTAAAAACTTTAGCAACAAATTGGTTACAAAATAAAGATTTTGTAACTTTTGAATATGAATATGGTAAAGATAAATCAAAAGATCAAAAGATTATTCATAAAAAATATTTTTATGAAACTATTACTTCAAATAAACAAATTTATGATTTTAATGAACCGGTTTTTTTATATACAGAAAACAAAAATAATGATATTAAATTATTAAAACTTGAAGATAAGAGTTTAATAATTGCTGCTTTAAAATCAATTAAACAACCACGAATAAAAAAATGA", "aa": "MRSFANEHDFILKTLATNWLQNKDFVTFEYEYGKDKSKDQKIIHKKYFYETITSNKQIYDFNEPVFLYTENKNNDIKLLKLEDKSLIIAALKSIKQPRIKK", "aa_hexdigest": "1854c131af622f0784a41367d18a3f37", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12170.863099999982, "isoelectric_point": 9.323498344421388 }, "id": "NKIECMJPJD_324", "locus": "GCA000828735_00288" }, { "type": "cds", "sequence": "contig_1", "start": 180406, "stop": 180612, "strand": "+", "frame": 1, "gene": "rpoE", "product": "DNA-directed RNA polymerase subunit delta", "db_xrefs": [ "COG:COG3343", "COG:K", "EC:2.7.7.6", "GO:0000428", "GO:0003899", "GO:0006351", "GO:0006355", "KEGG:K03048", "SO:0001217", "UniRef:UniRef50_Q9PPP2", "UniRef:UniRef90_Q9PPP2" ], "nt": "ATGAGCCGTCAATTAGTTGATATTGCTTATAATGCAATAAAAAATAATAAAATTTATAACAAAAAACCATTTACTTTTGATGATATTATTAATGAAATTGTCAAGAATAGTAATGTAAATTTAGTAGAAGTTAATTCACAATTAGGTGTTTTATATACAACTTTAATACAAGATACACGTTTTATTTCAATTGGAGATTTAGAATGA", "aa": "MSRQLVDIAYNAIKNNKIYNKKPFTFDDIINEIVKNSNVNLVEVNSQLGVLYTTLIQDTRFISIGDLE", "aa_hexdigest": "9703b99f5e6ae61f4301c6058f325428", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [ "rpoE" ], "truncated": "3-prime", "pseudogene": { "start": 180406, "stop": 180828, "observations": { "mutation": [ 180610 ], "stop": [ 180610 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PPP2", "query_cov": 1.0, "subject_cov": 0.4146341463414634, "identity": 1.0, "score": 135.0, "evalue": 4.25e-39, "gene_start": 1, "gene_end": 68, "reference_start": 1, "reference_end": 68, "reference_sequence": "MSRQLVDIAYNAIKNNKIYNKKPFTFDDIINEIVKNSNVNLVEVNSQLGVLYTTLIQDTRFISIGDLEWNLRERLSLDEITKINNAMYEVGLYKDSDREEDEHEIVKNDKLTQSKEQEESDEVSSLSDFVGYEDEDEEFKNNNTSNKDEIEDEEELEEELEEEE" }, "paralog": false, "identity": 0.9857142857142858, "score": 270.0, "evalue": 7.69e-95, "description": "Internal stop codon at 180610. Nonsense mutation around 180610.", "psc": { "uniref90_id": "UniRef90_Q9PPP2", "gene": "rpoE", "product": "DNA-directed RNA polymerase subunit delta", "ec_ids": [ "2.7.7.6" ], "uniref50_id": "UniRef50_Q9PPP2", "cog_id": "COG3343", "cog_category": "K", "kegg_orthology_id": "K03048", "go_ids": [ "GO:0000428", "GO:0003899", "GO:0006351", "GO:0006355" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PPP2", "EC:2.7.7.6", "UniRef:UniRef50_Q9PPP2", "COG:COG3343", "COG:K", "KEGG:K03048", "GO:0000428", "GO:0003899", "GO:0006351", "GO:0006355" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PPP2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PPP2" ], "product": "Probable DNA-directed RNA polymerase subunit delta" } }, "id": "NKIECMJPJD_325", "locus": "GCA000828735_00289" }, { "type": "cds", "sequence": "contig_1", "start": 180664, "stop": 180888, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00291355F0", "UniRef:UniRef90_UPI00291355F0" ], "nt": "ATGTATGAAGTTGGTTTATACAAAGATAGTGACCGTGAAGAAGATGAACATGAAATTGTTAAAAATGATAAATTAACACAATCAAAAGAACAAGAGGAAAGTGATGAAGTAAGCTCTTTATCTGATTTTGTTGGCTACGAAGATGAAGATGAAGAATTTAAAAATAATAATACTTCTAATAAAGATGAAATAGAAGACGAAGAGGAATTAGAAGAGGAAGAATAA", "aa": "MYEVGLYKDSDREEDEHEIVKNDKLTQSKEQEESDEVSSLSDFVGYEDEDEEFKNNNTSNKDEIEDEEELEEEE", "aa_hexdigest": "4c52f81b209b23671e051c28412918da", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00291355F0", "query_cov": 0.9459459459459459, "subject_cov": 0.8974358974358975, "identity": 0.914, "score": 82.8, "evalue": 2.29e-19, "valid": true, "uniref50_id": "UniRef50_UPI00291355F0" }, "pscc": { "uniref50_id": "UniRef50_UPI00291355F0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00291355F0" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8814.911800000002, "isoelectric_point": 4.0500284194946286 }, "id": "NKIECMJPJD_326", "locus": "GCA000828735_00290" }, { "type": "cds", "sequence": "contig_1", "start": 181064, "stop": 181450, "strand": "+", "frame": 2, "gene": null, "product": "class II fructose-bisphosphate aldolase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI003593948A" ], "nt": "ATGGGTGGTTATGATAATTGTTATGCAATGGTTGTTAATTTAATAAAACAAATGAATATTAAAACTCCTGTTTGTTTGCATTTAGATCATGGTACATATGAAGGATGTATAAAAGCTATTAATGCTGGTTATTCCTCAATTATGTATGATGGATCAAAACTTTCGATCGAAGAAAACATTAAAAACACTAAAGAACTTTTGACAATAACTAAATTAAAAGGTGTATCAGTAGAAGTTGAAGTTGGTTCAATTGGTGGGACAGAAGATGGGATTACTTCAGAAGGTGAATTAGCTAATATCGACGATTGTTATCAAATGTGTTTATTAGATATTGATATGTTAGCGTGTGGTATTGGAAATATTCACGGAATATATCCTAAAAATTGA", "aa": "MGGYDNCYAMVVNLIKQMNIKTPVCLHLDHGTYEGCIKAINAGYSSIMYDGSKLSIEENIKNTKELLTITKLKGVSVEVEVGSIGGTEDGITSEGELANIDDCYQMCLLDIDMLACGIGNIHGIYPKN", "aa_hexdigest": "bef560df32278171a6b639850d137462", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "psc": { "uniref90_id": "UniRef90_UPI003593948A", "query_cov": 0.8203125, "subject_cov": 0.8974358974358975, "identity": 0.514, "score": 112.0, "evalue": 7.48e-30, "valid": false, "product": "class II fructose-bisphosphate aldolase", "ec_ids": [ "4.1.2.13" ], "uniref50_id": "UniRef50_UPI003593948A", "kegg_orthology_id": "K01624" }, "pscc": { "uniref50_id": "UniRef50_UPI003593948A", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI003593948A" ], "product": "class II fructose-bisphosphate aldolase" }, "genes": [], "id": "NKIECMJPJD_327", "locus": "GCA000828735_00291" }, { "type": "cds", "sequence": "contig_1", "start": 181493, "stop": 181765, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01116.26" ], "nt": "GTGAATAAACCAATTGTTTTACACGGAGGTTCTGGTATTAGTGATGAACAAATTTTAAAAGCAATTAATTTGGGTGTAGCAAAAATTAATATTAATACAGAATGTCAAATTGCATTTTCAAATGCATTACAAGATCACTTAACAAAAGCAGGTGATTTAATTCTTTCTAAACAGTATGATCCACGAAAAATTTTGGCTTATGGTGTGGACGCAATTAAGAATACAATCATTGATAAATTCACTAAGTTTAATAGTTTAAATAAAATAAAATAA", "aa": "MNKPIVLHGGSGISDEQILKAINLGVAKININTECQIAFSNALQDHLTKAGDLILSKQYDPRKILAYGVDAIKNTIIDKFTKFNSLNKIK", "aa_hexdigest": "561444c70e1f6bca7382827cfff41777", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002BE51033", "query_cov": 0.9444444444444444, "subject_cov": 0.8854166666666666, "identity": 0.529, "score": 90.5, "evalue": 6.35e-22, "valid": false, "product": "class II fructose-bisphosphate aldolase", "ec_ids": [ "4.1.2.13" ], "uniref50_id": "UniRef50_A0A356BXS8", "kegg_orthology_id": "K01624" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01116.26", "name": "F_bP_aldolase", "length": 87, "aa_cov": 0.9666666666666667, "hmm_cov": 0.896551724137931, "evalue": 9.542551327476058e-23, "score": 76.60188293457031, "start": 2, "stop": 88 } ], "seq_stats": { "molecular_weight": 9895.41739999999, "isoelectric_point": 9.336649894714355 }, "id": "NKIECMJPJD_328", "locus": "GCA000828735_00292" }, { "type": "cds", "sequence": "contig_1", "start": 181776, "stop": 182663, "strand": "+", "frame": 3, "gene": "ychF", "product": "Ribosome-binding ATPase YchF", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P37518" ], "nt": "ATGTCGTTATCAGCAGGTCTTGTTGGTTTGCCAAACGTTGGTAAATCCACATTATTTAATACAATTACAAATTCTAGAGTTGAGGCAGCTAATTATCCTTTTGCAACTATCGAACCAAATATTGGAATTGTTAATATTAATGATCCGCGTTTAAAACGATTAGCAAGCTTAGTTGTGCCTGATAAAATTGTGCCGACCACTTTCAAATTTGTTGATATTGCAGGTCTTGTTAAAGGCGCTTCGCAAGGTGAAGGACTAGGAAACCAATTTTTAAATAATATTCGCGATGTTGATGCTATTTGTCATGTCGTTCGCTGTTTTGATGATAAATCTATTACTCATGTTCATAATAAAATTGACGCATTATCTGATATTCATGTTATTAATTATGAATTAATATTATCTGACCTTGATATTATTGAAAAACGAATTAGTCGTATTAAAAAAAAGGCTGATTCTGGCGATAAAGATGCTAGAAGAGAATATCTTATTTTATTTAACATTGAAGAAGCTTTAAAAAAGGGTCAATTAGCATCAAGTGTTAAATTAACCAAAGAAGAATTGAAAATCATTAATTCTTTCAATTTAATAACTTTAAAACCAATTTTATATATTGCAAATATTTCAGAAAATGAGATTTCAAATCCTGATTTAAATAAAGAATATTTAAAAGTAAAAAAGTATGCTCAAGATAATGATGCTAGATGCGTTGCTTTGTCAGCAAAAATTGAATATGAAATTAGCATTTTAGATGAAGAATCAAAATCAATATTTATTCAAGATTTAGGTATTAAAACAACAGGATTAGATGAAATTGTAAAGCAAACCTATGATTTATTAAATTTAAAAACTTATTTTACATATGGAAAACAAGAAGTACGTGCTTGA", "aa": "MSLSAGLVGLPNVGKSTLFNTITNSRVEAANYPFATIEPNIGIVNINDPRLKRLASLVVPDKIVPTTFKFVDIAGLVKGASQGEGLGNQFLNNIRDVDAICHVVRCFDDKSITHVHNKIDALSDIHVINYELILSDLDIIEKRISRIKKKADSGDKDARREYLILFNIEEALKKGQLASSVKLTKEELKIINSFNLITLKPILYIANISENEISNPDLNKEYLKVKKYAQDNDARCVALSAKIEYEISILDEESKSIFIQDLGIKTTGLDEIVKQTYDLLNLKTYFTYGKQEVRA", "aa_hexdigest": "4a9ae4ef85b5c7e2970944e1f0bd785e", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_Q9PPP4", "query_cov": 1.0, "subject_cov": 0.8016304347826086, "identity": 0.8170000000000001, "score": 475.0, "evalue": 1.3e-166, "valid": false, "gene": "ychF", "product": "redox-regulated ATPase YchF", "uniref50_id": "UniRef50_P37518", "cog_id": "COG0012", "cog_category": "J", "go_ids": [ "GO:0005524", "GO:0005525", "GO:0016887" ] }, "pscc": { "uniref50_id": "UniRef50_P37518", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P37518" ], "product": "Ribosome-binding ATPase YchF" }, "genes": [ "ychF" ], "id": "NKIECMJPJD_329", "locus": "GCA000828735_00293" }, { "type": "cds", "sequence": "contig_1", "start": 182679, "stop": 182882, "strand": "+", "frame": 3, "gene": null, "product": "Putative GTP-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0B7IGQ7" ], "nt": "ATGAGTGCGCCTGAATGTGCAGGAATTATTCATAGCGATTTTGAAAAAGGTTTTATTAAAGCTGAAATTATGCATTATGATGATTTAATATCATTAGGAAGTGAGCAAGCTGTGAAAGAAGCGGGAAAAATAAGAATGGAGGGAAAGTCATATTTAATGAAAGATGGCGATATTTGCAATTTTAGATTCAATGTGACAAAATAA", "aa": "MSAPECAGIIHSDFEKGFIKAEIMHYDDLISLGSEQAVKEAGKIRMEGKSYLMKDGDICNFRFNVTK", "aa_hexdigest": "a8244530075f4e53191e95f363c5a3f7", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A0B7IGQ7", "query_cov": 0.9552238805970149, "subject_cov": 0.8311688311688312, "identity": 0.672, "score": 97.8, "evalue": 1.9e-25, "valid": false, "product": "Putative GTP-binding protein", "uniref50_id": "UniRef50_A0A0B7IGQ7" }, "pscc": { "uniref50_id": "UniRef50_A0A0B7IGQ7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0B7IGQ7" ], "product": "Putative GTP-binding protein" }, "genes": [], "id": "NKIECMJPJD_330", "locus": "GCA000828735_00294" }, { "type": "cds", "sequence": "contig_1", "start": 182949, "stop": 183560, "strand": "+", "frame": 3, "gene": "tdk", "product": "thymidine kinase", "db_xrefs": [ "COG:COG1435", "COG:F", "EC:2.7.1.21", "GO:0004797", "GO:0005524", "GO:0005829", "GO:0008270", "GO:0046104", "GO:0071897", "KEGG:K00857", "SO:0001217", "UniRef:UniRef50_Q9PPP5", "UniRef:UniRef90_Q9PPP5" ], "nt": "ATGTTTGCTGGAAAAACAGCAGAGTTAATTCGTCGCTTACATCGTTTAGAATATGCAGATGTTAAATATTTGGTTTTTAAACCTAAAATTGATACTAGATCTATTAGAAATATCCAATCACGAACAGGAACGTCTTTACCATCAGTTGAAGTAGAAAGCGCACCAGAAATTTTAAATTATATTATGTCAAACAGTTTTAATGATGAAACAAAAGTTATTGGCATTGATGAGGTTCAATTTTTTGATGATCGGATTTGTGAAGTTGCAAATATTTTAGCAGAAAATGGTTTTGTTGTTATTATTTCAGGATTAGATAAAAATTTTAAAGGCGAGCCTTTTGGTCCAATTGCTAAACTTTTTACTTATGCTGATAAAATTACAAAACTAACAGCGATATGTAATGAGTGTGGCGCTGAAGCAACGCATTCATTGCGTAAGATTGATGGTAAACACGCTGACTATAATGATGATATTGTTAAAATTGGCTGTCAGGAATTCTATTCTGCTGTTTGTCGTCATCATCATAAGGTACCTAATCGTCCTTATTTAAATAGTAATTCTGAGGAATTTATTAAATTTTTTAAAAATAAAAAACGAAATAAAAATATATAA", "aa": "MFAGKTAELIRRLHRLEYADVKYLVFKPKIDTRSIRNIQSRTGTSLPSVEVESAPEILNYIMSNSFNDETKVIGIDEVQFFDDRICEVANILAENGFVVIISGLDKNFKGEPFGPIAKLFTYADKITKLTAICNECGAEATHSLRKIDGKHADYNDDIVKIGCQEFYSAVCRHHHKVPNRPYLNSNSEEFIKFFKNKKRNKNI", "aa_hexdigest": "89ef5dc5465ba9f0e2bb9d77f37d7a29", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_Q9PPP5", "query_cov": 1.0, "subject_cov": 0.8353909465020576, "identity": 1.0, "score": 412.0, "evalue": 4.96e-145, "valid": true, "gene": "tdk", "product": "thymidine kinase", "ec_ids": [ "2.7.1.21" ], "uniref50_id": "UniRef50_Q9PPP5", "cog_id": "COG1435", "cog_category": "F", "kegg_orthology_id": "K00857", "go_ids": [ "GO:0004797", "GO:0005524", "GO:0005829", "GO:0008270", "GO:0046104", "GO:0071897" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PPP5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PPP5" ], "product": "Thymidine kinase" }, "genes": [ "tdk" ], "id": "NKIECMJPJD_331", "locus": "GCA000828735_00295" }, { "type": "cds", "sequence": "contig_1", "start": 183813, "stop": 184037, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T0B4", "UniRef:UniRef90_A0AAC9T0B4" ], "nt": "ATGTTATTTATTACAATAATTTATCTTTTATGGATCAATTGTCATAATAGCACAGCAAGATATTTAATTTTAAAAATCAATAAATACCCACCGAAAAAACCGATCAAAAATCTTCCTTTTATTTTTTTTAGATTAGCTTATTATACATTTAATATAAGACAAAAAATTCAATATCAACAAAAACAAATTTATAAATATTTAATATCTTTTAATGAGTATGTGTAG", "aa": "MLFITIIYLLWINCHNSTARYLILKINKYPPKKPIKNLPFIFFRLAYYTFNIRQKIQYQQKQIYKYLISFNEYV", "aa_hexdigest": "9dc52ed2281ba2740fc7b24e17702657", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 183597, "stop": 184037, "observations": { "mutation": [ 183696 ], "stop": [ 183696 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAC9T0B4", "query_cov": 1.0, "subject_cov": 0.5068493150684932, "identity": 1.0, "score": 150.0, "evalue": 2.62e-45, "gene_start": 1, "gene_end": 74, "reference_start": 73, "reference_end": 146, "reference_sequence": "MKIVLEELKDYLIGKLYFKYKFIGVFISIFLAGWLISFITNLILALNYGYNEKLLNYKKYLDFTILTCISFLMLFITIIYLLWINCHNSTARYLILKINKYPPKKPIKNLPFIFFRLAYYTFNIRQKIQYQQKQIYKYLISFNEYV" }, "paralog": false, "identity": 0.9863013698630136, "score": 292.0, "evalue": 7.86e-104, "description": "Internal stop codon at 183696. Nonsense mutation around 183696.", "psc": { "uniref90_id": "UniRef90_A0AAC9T0B4", "uniref50_id": "UniRef50_A0AAC9T0B4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAC9T0B4", "UniRef:UniRef50_A0AAC9T0B4" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAC9T0B4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T0B4" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 9245.982, "isoelectric_point": 9.956448936462401 }, "id": "NKIECMJPJD_332", "locus": "GCA000828735_00296" }, { "type": "cds", "sequence": "contig_1", "start": 184091, "stop": 184258, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAGCAACAGATGAGATTTTAAAAAATCTTAAAAATGTTGATTTTTTTATTCAATTAGTTGATGCTAGATGTCCATTAACATCAAGTAATATTGAATTATTAAAAGAAGTTTCATCCAAACCAGTTATTAATTTGGCTAATAAGGCCGATTTAAGTGATTGA", "aa": "MKKATDEILKNLKNVDFFIQLVDARCPLTSSNIELLKEVSSKPVINLANKADLSD", "aa_hexdigest": "cff49dbbc4105b08a8b2bd5e21dfe2b5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6132.066299999999, "isoelectric_point": 6.143294715881347 }, "id": "NKIECMJPJD_333", "locus": "GCA000828735_00297" }, { "type": "cds", "sequence": "contig_1", "start": 184406, "stop": 184870, "strand": "+", "frame": 2, "gene": "ylqF", "product": "ribosome biogenesis GTPase YlqF", "db_xrefs": [ "COG:COG1161", "COG:J", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0042254", "SO:0001217", "UniRef:UniRef50_A0AAP9D7M6", "UniRef:UniRef90_Q9PPP7" ], "nt": "ATGATTATTGGTCTACCAAATATTGGTAAGTCTTCTTTGATTAATTTTTTAGCTCCCAAAAAAGTATTAAAAGTTGAAAATAGACCTGGAGTTACTAAGACTCAATGTATTCGTCAAATCAATCAACATTTTTATTTAATTGATACACCTGGAATTTTTTTAAAAAATATTCAAAATGAGCGTGATAGTTTTGTTTTGACTTTGATTAATTGTGTTAAGAAAGAAGTTTTGGAATTAGAATCAATTGTACATTTTGCATATGATTTTTATTTAAAAAATTATAGGAATGAATTATTTATAAAATATAAAATGAATAAGGTATTAAATTTTGATGATTTTATTCATCATATTTGTGAGTTATATAATTATAAATTAACAAATAATGAATTTGATCATGTAAGAGCTTATGAGAATTTATTTAATGATTTTTCTAATGGATTGATTTGTAAAGTTAATTATGATTAG", "aa": "MIIGLPNIGKSSLINFLAPKKVLKVENRPGVTKTQCIRQINQHFYLIDTPGIFLKNIQNERDSFVLTLINCVKKEVLELESIVHFAYDFYLKNYRNELFIKYKMNKVLNFDDFIHHICELYNYKLTNNEFDHVRAYENLFNDFSNGLICKVNYD", "aa_hexdigest": "3dc1376187c3eb336ce41b9d4ce99dd8", "start_type": "ATG", "rbs_motif": null, "genes": [ "ylqF" ], "truncated": "5-prime", "pseudogene": { "start": 184106, "stop": 184870, "observations": { "mutation": [ 184256 ], "stop": [ 184256 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PPP7", "query_cov": 1.0, "subject_cov": 0.5641025641025641, "identity": 1.0, "score": 314.0, "evalue": 6.6e-107, "gene_start": 1, "gene_end": 154, "reference_start": 120, "reference_end": 273, "reference_sequence": "MDIINKKINWFPGHMKKATDEILKNLKNVDFFIQLVDARCPLTSSNIELLKEVSSKPVINLANKADLSDWKKNFDNNFLLISTKKRNDKNKIIQHLYQLFDQKIKIYQKKGLINPKFVGMIIGLPNIGKSSLINFLAPKKVLKVENRPGVTKTQCIRQINQHFYLIDTPGIFLKNIQNERDSFVLTLINCVKKEVLELESIVHFAYDFYLKNYRNELFIKYKMNKVLNFDDFIHHICELYNYKLTNNEFDHVRAYENLFNDFSNGLICKVNYD" }, "paralog": false, "identity": 0.9960629921259843, "score": 512.0, "evalue": 2.65e-187, "description": "Internal stop codon at 184256. Nonsense mutation around 184256.", "psc": { "uniref90_id": "UniRef90_Q9PPP7", "gene": "ylqF", "product": "ribosome biogenesis GTPase YlqF", "uniref50_id": "UniRef50_A0AAP9D7M6", "cog_id": "COG1161", "cog_category": "J", "go_ids": [ "GO:0003924", "GO:0005525", "GO:0005737", "GO:0042254" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PPP7", "UniRef:UniRef50_A0AAP9D7M6", "COG:COG1161", "COG:J", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0042254" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAP9D7M6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAP9D7M6" ], "product": "Ribosome biogenesis GTPase A" } }, "id": "NKIECMJPJD_334", "locus": "GCA000828735_00298" }, { "type": "cds", "sequence": "contig_1", "start": 185212, "stop": 185631, "strand": "+", "frame": 1, "gene": "smf", "product": "Unique hypothetical", "db_xrefs": [ "COG:COG0758", "COG:L", "SO:0001217", "UniRef:UniRef50_Q9PPP8", "UniRef:UniRef90_Q9PPP8" ], "nt": "TTGATAAAAGCAGGATATGGATTTGTTATTTATTATGATAGTGTTGTTTTGGACACACTTATCAATCGTTTAATTTCTCTTAAAGCTAGAATATGTATCATCACAGATGAAGGAATCTATAGTTCTAAAAACATTAAATACTTAAGTTACTGCAATGTTAGTGTAATATCTGAAATTCCAAAAGATTACAATAGTAATAAAATTACACAGGATTTAAAACGTCTTGCATGCGCTTCAAGCGACCATATTTTATGTGTTAATGAGTTATATCAAATAGATGATTATCTTTTTAATAAATATAAAGATTGCCAATTAGATTTGGTGTTATTAGATAAAAAATTAGCTAAGCAAAACACAATTTATCCCAAATACATTAATCGTATTTATCAATTTATTAAAAATTCAAAATTAATAAATTAA", "aa": "MIKAGYGFVIYYDSVVLDTLINRLISLKARICIITDEGIYSSKNIKYLSYCNVSVISEIPKDYNSNKITQDLKRLACASSDHILCVNELYQIDDYLFNKYKDCQLDLVLLDKKLAKQNTIYPKYINRIYQFIKNSKLIN", "aa_hexdigest": "b5006b1b8bd604b3b8772e2041ee4285", "start_type": "TTG", "rbs_motif": null, "genes": [ "smf" ], "truncated": "5-prime", "pseudogene": { "start": 184912, "stop": 185631, "observations": { "mutation": [ 184966, 185143 ], "stop": [ 184966, 185143 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PPP8", "query_cov": 1.0, "subject_cov": 0.5815899581589958, "identity": 0.993, "score": 277.0, "evalue": 4.61e-93, "gene_start": 1, "gene_end": 139, "reference_start": 101, "reference_end": 239, "reference_sequence": "MIPLNYLTHYFSLKYKGDWSKIYLALINHEDQNEYLFLEKEDYEKVKAIDLTDEKYPNKYKKINQPPFILYYSTTVEWLDKKNIWLIDYENAISNYDILTLIKAGYGFVIYYDSVVLDTLINRLISLKARICIITDEGIYSSKNIKYLSYCNVSVISEIPKDYNSNKITQDLKRLACASSDHILCVNELYQIDDYLFNKYKDCQLDLVLLDKKLAKQNTIYPKYINRIYQFIKNSKLIN" }, "paralog": false, "identity": 0.9874476987447699, "score": 477.0, "evalue": 2.17e-174, "description": "Internal stop codon at 184966, 185143. Nonsense mutation around 184966, 185143.", "psc": { "uniref90_id": "UniRef90_Q9PPP8", "gene": "Smf", "product": "Unique hypothetical", "uniref50_id": "UniRef50_Q9PPP8", "cog_id": "COG0758", "cog_category": "L", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PPP8", "UniRef:UniRef50_Q9PPP8", "COG:COG0758", "COG:L" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PPP8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PPP8" ], "product": "Unique hypothetical" } }, "id": "NKIECMJPJD_335", "locus": "GCA000828735_00299" }, { "type": "cds", "sequence": "contig_1", "start": 185706, "stop": 185861, "strand": "+", "frame": 3, "gene": null, "product": "toprim domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000A54A148" ], "nt": "ATGCAAAAAGATTTAATAGTAGTAGAGTCACCAAATAAAATTAAATCAATTCAGTCTTATTTAGGTGATAGTTATAATGTAATAGCTACATGTGGACATTTACGTGAGCTCGCAAAAAAATCTGGTTATGATCCAAAAACTTATGATTTAAAATGA", "aa": "MQKDLIVVESPNKIKSIQSYLGDSYNVIATCGHLRELAKKSGYDPKTYDLK", "aa_hexdigest": "dbfadc68afcddc005a4213e31fd1059e", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_UPI000A54A148", "query_cov": 0.9411764705882353, "subject_cov": 0.8275862068965517, "identity": 0.7709999999999999, "score": 80.9, "evalue": 3.05e-19, "valid": false, "product": "toprim domain-containing protein", "uniref50_id": "UniRef50_UPI000A54A148" }, "pscc": { "uniref50_id": "UniRef50_UPI000A54A148", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000A54A148" ], "product": "toprim domain-containing protein" }, "genes": [], "id": "NKIECMJPJD_336", "locus": "GCA000828735_00300" }, { "type": "cds", "sequence": "contig_1", "start": 186114, "stop": 186287, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTAGATTCGCAATTTGCAAGACGAATTTTAGATCGAGTTATTGGTTATAAACTTTCTGGATTAGTTAAAAAAAACTTACATGGCTTATCTGCTGGGCGAGTACAATCGATCGCTTTATTATTTGTAGTTGAGAGAGAGTTAGAAAGACGTTCTTTTGTTAAAGAAGAATGA", "aa": "MVDSQFARRILDRVIGYKLSGLVKKNLHGLSAGRVQSIALLFVVERELERRSFVKEE", "aa_hexdigest": "6dc441dd1fd88b7668d5e0a64d06611a", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6558.616099999999, "isoelectric_point": 10.141988945007324 }, "id": "NKIECMJPJD_337", "locus": "GCA000828735_00301" }, { "type": "cds", "sequence": "contig_1", "start": 186423, "stop": 186551, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATTGATGATTTAAGTGATATTTTTAAAGTTTATAGTATTGATGATTTGAAAATCACCAAAGGCGATGTTCAATTACCATTAACAACTGATAAATTATTACAAATCGCATCCAATAATTTTGGTTGA", "aa": "MIDDLSDIFKVYSIDDLKITKGDVQLPLTTDKLLQIASNNFG", "aa_hexdigest": "82d4722100f64144a16398f96b305bda", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4699.3315999999995, "isoelectric_point": 4.219522285461426 }, "id": "NKIECMJPJD_338", "locus": "GCA000828735_00302" }, { "type": "cds", "sequence": "contig_1", "start": 186585, "stop": 186872, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTGAAGGATTAGAAATTAATAATCAGCAATTAGCTTTAATAACTTATCCAAGAACAGATAGCGAGCGATTAAGTGATGATTTTTTACAAGAAGCTAATGCCTTTATTGAAAATAATTTTGGTAAAGAATATCTAGAAAATTTTAAAAATAAAAAAATTAATAGAAAAGAAGTAAATATTCAAGACGCACATGAAGCAATTCGCCCTGTTGATATTAATTTAAAACCTGAAGAAATTAAAGATTATGTTTCATTAGATATTTATAAGCTGTATAACTTAGTTTGA", "aa": "MFEGLEINNQQLALITYPRTDSERLSDDFLQEANAFIENNFGKEYLENFKNKKINRKEVNIQDAHEAIRPVDINLKPEEIKDYVSLDIYKLYNLV", "aa_hexdigest": "51608af05319ad1fd7ea31989540cf77", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11252.513799999988, "isoelectric_point": 4.729879570007324 }, "id": "NKIECMJPJD_339", "locus": "GCA000828735_00303" }, { "type": "cds", "sequence": "contig_1", "start": 186894, "stop": 186992, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGTGCCAACTTTTAATAAGCAAGTAATTCGTTTTATTAATAATGAATATAAATTTTATACAGCTTATAAAACAATTCGAACTTTAGGATATTGA", "aa": "MKVPTFNKQVIRFINNEYKFYTAYKTIRTLGY", "aa_hexdigest": "213444f41ed683ceb3167793a0a392b3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3948.589500000001, "isoelectric_point": 9.9959680557251 }, "id": "NKIECMJPJD_340", "locus": "GCA000828735_00304" }, { "type": "cds", "sequence": "contig_1", "start": 187188, "stop": 187370, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAACTATTGGCACCACTAGGGGTTACATCAATAAAGATAAGAGTAAATTAATTCCAACAGAATTAGGGATTAAAGTCATCCAAGAGTTAAAAGAAGCCTTTCCAAAAGTGATTTCTATTAAATTTACATCTGAGATGGAAGAACGTTTAGATGTTATAGCTAATGGCAAATTATTTTGA", "aa": "MATIGTTRGYINKDKSKLIPTELGIKVIQELKEAFPKVISIKFTSEMEERLDVIANGKLF", "aa_hexdigest": "6271983b6bb0a7e320acfb890dc9b8ff", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6754.907399999998, "isoelectric_point": 9.099599647521973 }, "id": "NKIECMJPJD_341", "locus": "GCA000828735_00305" }, { "type": "cds", "sequence": "contig_1", "start": 187512, "stop": 187739, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01396.24" ], "nt": "ATGAACACTCGGTTCATTGCGTGTTCAAATTTCCCTACATGTCGTTTTACAGAATCGTTAGAGAAACCAGAAATTCTTAACGAATTATGTCCTAACTGTTCAAAATCATTGGTTAAACGTAAAAATCGTAAAAATAAATATTTTATTGGATGCACAGGTTATCCTGAATGTAATTTTATTCGTAATTTAGATGAATCAAACAATGATAAAAACGATTTTAATCAATAA", "aa": "MNTRFIACSNFPTCRFTESLEKPEILNELCPNCSKSLVKRKNRKNKYFIGCTGYPECNFIRNLDESNNDKNDFNQ", "aa_hexdigest": "6b7ecdc6eb6953975f3e12c1b94511b1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01396.24", "name": "Zn_ribbon_Top1", "length": 38, "aa_cov": 0.5066666666666667, "hmm_cov": 0.9736842105263158, "evalue": 8.084366344375403e-22, "score": 72.38121032714844, "start": 28, "stop": 65 } ], "seq_stats": { "molecular_weight": 8803.891899999995, "isoelectric_point": 8.591717720031738 }, "id": "NKIECMJPJD_342", "locus": "GCA000828735_00306" }, { "type": "cds", "sequence": "contig_1", "start": 187768, "stop": 187860, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAATTAAATTAGAAAGTAACCAAAATTTAAATGAATTATTAAAGGAAAATCATTCTAAACCAATATTAATTGATTTTTATGCGGATTGA", "aa": "MLIKLESNQNLNELLKENHSKPILIDFYAD", "aa_hexdigest": "fe0110f0c5f1e3eff85c0f822d21b168", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3544.0370000000003, "isoelectric_point": 5.0082201004028315 }, "id": "NKIECMJPJD_343", "locus": "GCA000828735_00307" }, { "type": "cds", "sequence": "contig_1", "start": 187876, "stop": 188088, "strand": "+", "frame": 1, "gene": "trxA", "product": "thioredoxin", "db_xrefs": [ "COG:COG3118", "COG:O", "GO:0015035", "SO:0001217", "UniRef:UniRef50_Q9PPQ0", "UniRef:UniRef90_Q9PPQ0" ], "nt": "ATGCTTATTCCAGTATTAGATTCAATTGAAAAAAAACATGGTGATGAATTTACTATTATTAAAATAAATGTTGATCATTTTCCAGAATTATCAACACAATATCAAGTCAAATCAATCCCTGCTTTATTTTACTTAAAAAATGGAGATATTAAAGCGACAAGTTTAGGATTTATTGATGAAAATTCTTTAGTTAATAAATTACGATCAATTTAA", "aa": "MLIPVLDSIEKKHGDEFTIIKINVDHFPELSTQYQVKSIPALFYLKNGDIKATSLGFIDENSLVNKLRSI", "aa_hexdigest": "22d1e93dd80a069156da51cb1e62d52e", "start_type": "ATG", "rbs_motif": null, "genes": [ "trxA" ], "truncated": "5-prime", "pseudogene": { "start": 187768, "stop": 188088, "observations": { "mutation": [ 187858 ], "stop": [ 187858 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PPQ0", "query_cov": 1.0, "subject_cov": 0.660377358490566, "identity": 1.0, "score": 140.0, "evalue": 4.17e-42, "gene_start": 1, "gene_end": 70, "reference_start": 37, "reference_end": 106, "reference_sequence": "MLIKLESNQNLNELLKENHSKPILIDFYADWCPPCRMLIPVLDSIEKKHGDEFTIIKINVDHFPELSTQYQVKSIPALFYLKNGDIKATSLGFIDENSLVNKLRSI" }, "paralog": false, "identity": 0.9905660377358491, "score": 211.0, "evalue": 1.45e-72, "description": "Internal stop codon at 187858. Nonsense mutation around 187858.", "psc": { "uniref90_id": "UniRef90_Q9PPQ0", "gene": "trxA", "product": "thioredoxin", "uniref50_id": "UniRef50_Q9PPQ0", "cog_id": "COG3118", "cog_category": "O", "go_ids": [ "GO:0015035" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PPQ0", "UniRef:UniRef50_Q9PPQ0", "COG:COG3118", "COG:O", "GO:0015035" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PPQ0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PPQ0" ], "product": "Thioredoxin" } }, "id": "NKIECMJPJD_344", "locus": "GCA000828735_00308" }, { "type": "tRNA", "sequence": "contig_1", "start": 188178, "stop": 188266, "strand": "+", "gene": "trnS", "product": "tRNA-Ser(gct)", "amino_acid": "Ser", "anti_codon": "gct", "score": 78.5, "nt": "GGAAACATACTCAAGTGGTTAAGAGGGCACCCTGCTAAGGTGTTAGGTCGTTCTGCGGCGCGTGGGTTCGAATCCCACTGTTTCCGCCA", "db_xrefs": [ "SO:0000269" ], "anti_codon_pos": [ 188211, 188213 ], "id": "NKIECMJPJD_1", "locus": "GCA000828735_00309" }, { "type": "cds", "sequence": "contig_1", "start": 188345, "stop": 188551, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAACGTATTTTAGCCAAATTCATCATAACATTTATTTGTGTATTATTAATAATTAATATTTGTTTAATATCAATATATTTTCACCATCGACCAACGGTTATTGTTACAGATATTTTAATATCATTTTATATTTTAAATACTAGTATTGGCTTATATATTTTATCTTCAAGACATCGGCAAGATCGTTCTAAAAAATCATGA", "aa": "MKKRILAKFIITFICVLLIINICLISIYFHHRPTVIVTDILISFYILNTSIGLYILSSRHRQDRSKKS", "aa_hexdigest": "b226293d2fb5740e67d5062b02735baa", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7992.647899999995, "isoelectric_point": 10.228569984436035 }, "id": "NKIECMJPJD_345", "locus": "GCA000828735_00310" }, { "type": "cds", "sequence": "contig_1", "start": 188579, "stop": 188911, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTCAATTATATTCTTTTGGATTTTTGGATCACGCCCATTTAAAGATAAAAAAATTAATGATATCATTACAAACCGTCGTGCACTTTTTAAATTTGAAGATTATGAATTTTATCATAGTTTTCAAAATATGATTATTAATAATAATGGACTTAATTTAATTCGTAAAATTGGTCAGTATAATTACCAACTTTTTCGTACACCTATTTATTGTGATAATCAAATAACAATTATTAAAGAGACAAACGAAATGTATGAACAAACTATTAAATTAATAAGAAAAGCAAAAAAAACAATTTATTTACAATATTATATTATTGAGAATGGTCGTTGA", "aa": "MSIIFFWIFGSRPFKDKKINDIITNRRALFKFEDYEFYHSFQNMIINNNGLNLIRKIGQYNYQLFRTPIYCDNQITIIKETNEMYEQTIKLIRKAKKTIYLQYYIIENGR", "aa_hexdigest": "efc83defcf7d662f53bc31e19dacca7a", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13526.565399999983, "isoelectric_point": 9.574667167663574 }, "id": "NKIECMJPJD_346", "locus": "GCA000828735_00311" }, { "type": "cds", "sequence": "contig_1", "start": 189329, "stop": 189604, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13091.12" ], "nt": "ATGCATCCTCAACCTTACTTATGTCAAGGAAAAGATGACTTAATAAATAATTCTATTAAAAATAAAACAATAACAACACTTTTGCATACAGGACCAGACCAATCAACAAGTTCTTTAAATGAAATTCTGGTTATGGCAATCGCTAATGCTAAAAAATCAATTACAATTTTTACGCCATATTTATTTCCTACTGAAGATATTTTAATAGGACTTAAAGCTGCAGCGAATACTGGAATAAAAGTTAAAATTATTTTACCTGGAAGGGTTGATTCTTGA", "aa": "MHPQPYLCQGKDDLINNSIKNKTITTLLHTGPDQSTSSLNEILVMAIANAKKSITIFTPYLFPTEDILIGLKAAANTGIKVKIILPGRVDS", "aa_hexdigest": "1bf27ebb30228a55fd74795d162bb131", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13091.12", "name": "PLDc_2", "length": 44, "aa_cov": 0.4835164835164835, "hmm_cov": 1.0, "evalue": 4.3851859545512816e-10, "score": 35.099185943603516, "start": 44, "stop": 87 } ], "seq_stats": { "molecular_weight": 9876.429299999989, "isoelectric_point": 8.819871330261233 }, "id": "NKIECMJPJD_347", "locus": "GCA000828735_00312" }, { "type": "cds", "sequence": "contig_1", "start": 189620, "stop": 189943, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13091.12" ], "nt": "ATGAATCGTTTAAGTTATGAATCATTAATTAAGTCCGGTGTACAAATTTATGAATATAGTGGTTTTTTACACACTAAAGCGATTTTAATAGATGATGATATTTCACTATTAGGTTCTTATAATCTTGATAATAGAGCATTAGTAATTAATTACGAATCATTATTAACAATTTATTCAATACAAGTAAATCACGACATTAACGAGATTTTTAATCAATATCTCCGTAATTCACAGTTAATTAATTTAGAAGATTTAGATAACAAATTCACATTCAACGAATTAGTTCAAACAGTATTTATTAATATTTTTCAATTACTTTTATAA", "aa": "MNRLSYESLIKSGVQIYEYSGFLHTKAILIDDDISLLGSYNLDNRALVINYESLLTIYSIQVNHDINEIFNQYLRNSQLINLEDLDNKFTFNELVQTVFINIFQLLL", "aa_hexdigest": "9dd3e8b9294ffea2edef682a9f8d8094", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13091.12", "name": "PLDc_2", "length": 72, "aa_cov": 0.6448598130841121, "hmm_cov": 1.0, "evalue": 4.026044534069161e-18, "score": 61.104087829589844, "start": 5, "stop": 73 } ], "seq_stats": { "molecular_weight": 12525.082899999987, "isoelectric_point": 4.456313514709472 }, "id": "NKIECMJPJD_348", "locus": "GCA000828735_00313" }, { "type": "cds", "sequence": "contig_1", "start": 190043, "stop": 190306, "strand": "+", "frame": 2, "gene": "ptsH", "product": "Phosphocarrier protein HPr", "db_xrefs": [ "COG:COG1925", "COG:TG", "RefSeq:WP_006688423.1", "SO:0001217", "UniParc:UPI00000C1CD4", "UniRef:UniRef100_Q9PPQ2", "UniRef:UniRef50_Q9PPQ2", "UniRef:UniRef90_Q9PPQ2" ], "nt": "ATGATATCGAAAGAATTTACCATCGTAGCAGAATTGTCAGCATTTATTAAGGCGATCACTTATTTTGTTAATGCAGCTAGTGATTTTAGCTCTTCAATTACTATTAAAGCAAATGGGCGTCAAGCAGATGCTAAATCAATTATCAACATTATGGCTTTAGGAATTAAACAAGGCACAAAAATCGAGCTGAGTGCCGTTGGAAATGATGCAAATAAAGCAATTAACAAATTAGAAGAAATTTTAATTGAACAGAAATTAATTTAA", "aa": "MISKEFTIVAELSAFIKAITYFVNAASDFSSSITIKANGRQADAKSIINIMALGIKQGTKIELSAVGNDANKAINKLEEILIEQKLI", "aa_hexdigest": "453cc491f882c2d70f0befdeb2de86ca", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI00000C1CD4", "ncbi_nrp_id": "WP_006688423.1", "uniref100_id": "UniRef100_Q9PPQ2", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1CD4", "RefSeq:WP_006688423.1", "UniRef:UniRef100_Q9PPQ2" ] }, "ips": { "uniref100_id": "UniRef100_Q9PPQ2", "uniref90_id": "UniRef90_Q9PPQ2" }, "psc": { "uniref90_id": "UniRef90_Q9PPQ2", "gene": "PtsH", "product": "Phosphocarrier protein HPr", "uniref50_id": "UniRef50_Q9PPQ2", "cog_id": "COG1925", "cog_category": "TG" }, "pscc": { "uniref50_id": "UniRef50_Q9PPQ2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PPQ2" ], "product": "Phosphocarrier protein HPr" }, "genes": [ "ptsH" ], "id": "NKIECMJPJD_349", "locus": "GCA000828735_00314" }, { "type": "cds", "sequence": "contig_1", "start": 190336, "stop": 190488, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00456.26" ], "nt": "ATGAATCGTTATGTTAACGCAATGCGTTCACTTGCTTTACAGGCTATTAATAAAGCAAAGCAAGGTCACAGTGGAATGAGTATTTCAGCTGCACCTATTGTTTATACCCTTTATAAGGGATTAATGACTATTTCAAAATCACATCCAAAGTGA", "aa": "MNRYVNAMRSLALQAINKAKQGHSGMSISAAPIVYTLYKGLMTISKSHPK", "aa_hexdigest": "45d8c5fe165557a647d5ccec723ef389", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00456.26", "name": "Transketolase_N", "length": 45, "aa_cov": 0.9, "hmm_cov": 1.0, "evalue": 2.3217552702069096e-09, "score": 32.19496536254883, "start": 2, "stop": 46 } ], "seq_stats": { "molecular_weight": 5478.4427, "isoelectric_point": 10.461107444763183 }, "id": "NKIECMJPJD_350", "locus": "GCA000828735_00315" }, { "type": "cds", "sequence": "contig_1", "start": 190504, "stop": 190950, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00456.26" ], "nt": "TTGGTTTTATCAGCTGGTCATGGATCGATGGCATTATATCCTGTTTTTTATTTTTCATCATTATTAACATTAGATGACATTAAAAATTTTCGCAATGATAATTATTTAACACCAGGTCATCCTGAAGTGTTGGCTAATAATTATATTGATGCATCAACAGGACCACTAGGGCAAGGGGTTGCTAATGCAGTTGGTATGGCAATTACTGAAAGTTATTTAAGAACTGAATTTGCTACTCTTAAAGGTGTTATTGATCATTATACTTATTGTATTGTTGGCGATGGGGACCTACAAGAGGGAATTTGTTATGAGGCAATGAGTATTGCTGGAAAATTAAAATTATCGAAATTAATTATTTTACATGATTCAAATGATTACCAATTAGATTCAGCAGTAAGTGATGTTAATATTGAAGATTTAAAAATGCGTGTTGAATCAATGGGTTGA", "aa": "MVLSAGHGSMALYPVFYFSSLLTLDDIKNFRNDNYLTPGHPEVLANNYIDASTGPLGQGVANAVGMAITESYLRTEFATLKGVIDHYTYCIVGDGDLQEGICYEAMSIAGKLKLSKLIILHDSNDYQLDSAVSDVNIEDLKMRVESMG", "aa_hexdigest": "2b7b40f0304056455a264faddd5146cb", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00456.26", "name": "Transketolase_N", "length": 148, "aa_cov": 0.9864864864864865, "hmm_cov": 1.0, "evalue": 2.3125339993116867e-47, "score": 157.1142578125, "start": 2, "stop": 147 } ], "seq_stats": { "molecular_weight": 16148.13909999999, "isoelectric_point": 4.542254066467286 }, "id": "NKIECMJPJD_351", "locus": "GCA000828735_00316" }, { "type": "cds", "sequence": "contig_1", "start": 191263, "stop": 192159, "strand": "+", "frame": 1, "gene": "tktA", "product": "transketolase", "db_xrefs": [ "COG:COG0021", "COG:G", "EC:2.2.1.1", "SO:0001217", "UniRef:UniRef50_A0A084EYA4", "UniRef:UniRef90_Q9PPQ3" ], "nt": "ATGCAAACTAATCCAGAACAAATGCAACGTTTATTAAATTATATTAATGGTAATTATGAAGATTTAAATAAATTATTAGATGAAAATAAAATTACTAATTTAAATGATTCTACTCGCAGTTATTTAAAACAATATTTTAATCAATTAAAAGATTTAAAATCAGCTCTTGTTTTATCTGCTGATTTAGCTAAATCAACTTTCACAAAAATAGGCGAAGGCGCTTTTAACGATAACCATAAAAATCCATATATTAAATTCGGTATTCGTGAATTTGCTATGGCAGGAGCGATGAATGGAATAAGTTTACATCAAGGCGTTAAAGCGATAGGAGGCACTTTTTTAGCTTTTTCTGATTATATGAAACCAGCAATTCGTTTAGCTGCCATTAGTAATTTAGCTAATTTATTTATTTTTAGTCACGATAGTTATGCAGTGGGTGGTGATGGACCTACACATCAACCTGTCGATCAATTACCAATGTTAAGAGCAATTCCAAATGTAGAAGTAATTCGTCCTGCTGATCATTATGAAGTTAAATATGCTCTTTCATATAGTTTTAAACAAAAACAAAAACCGATTTGTTTAATTACTTCACGTCAAACTATTAAACAAATTAATGATCAAAAACCACAAGATTTTAGTAAGGGAGCATATATTATTAATTCGCCTTTTTCTTTTTGTAAAGATGTAGATTACACGATTATTGCTAGTGGTAGTGAAGTTAGTTTAGCAAATGACGCAGCAAAAGAACTTTTTGAAAAACATAAACTTAAAATTAAAGTTATTTCTGCTTTTAACTTAAATTTATTTTTACGTCAAAAACCTGAAGATATTAAGAGCCTTTTAGCTTCAAAAAATGGTCTATTAGCAATTGAAGCATCAAGCGAAATGCTTTGA", "aa": "MQTNPEQMQRLLNYINGNYEDLNKLLDENKITNLNDSTRSYLKQYFNQLKDLKSALVLSADLAKSTFTKIGEGAFNDNHKNPYIKFGIREFAMAGAMNGISLHQGVKAIGGTFLAFSDYMKPAIRLAAISNLANLFIFSHDSYAVGGDGPTHQPVDQLPMLRAIPNVEVIRPADHYEVKYALSYSFKQKQKPICLITSRQTIKQINDQKPQDFSKGAYIINSPFSFCKDVDYTIIASGSEVSLANDAAKELFEKHKLKIKVISAFNLNLFLRQKPEDIKSLLASKNGLLAIEASSEML", "aa_hexdigest": "53b300d1a16cca878c730575d1fb3f64", "start_type": "ATG", "rbs_motif": null, "genes": [ "tktA" ], "truncated": "both", "pseudogene": { "start": 190963, "stop": 192297, "observations": { "mutation": [ 191011, 191239, 192157, 192160 ], "stop": [ 191011, 191239, 192157, 192160 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PPQ3", "query_cov": 1.0, "subject_cov": 0.45565749235474007, "identity": 0.9129999999999999, "score": 534.0, "evalue": 1.43e-185, "gene_start": 1, "gene_end": 298, "reference_start": 310, "reference_end": 607, "reference_sequence": "MNRYVNAMRSLALQAINKANQGHSGMSISAAPIIYTLYKGLMTISKSHPKWFNRDRLVLSAGHGSMALYPVFYFSSLLTLDDIKNFRNDNHLTPGHPEVLSNNYIDASTGPLGQGVANAVGMAITESYLRAEFASLKGVVDHYTYCIVGDGDLQEGISYEAMSIAGKLKLSKLIILHDSNDYQLDSAVSDVNIEDLKMRVESMGWNYLKTDNNPENIFKAIAEAKWKKNVKPTFIEVKTIIGEGTSFENSNEAHAAAISKEELEKFGKRFHTKTNNFEFHQEIFDHFFFNVVARGESAYNQWQQLVDQYMQTNPEQMQRLLNYINGNYEDLNKMLDENKIVNLSDSTRSYLKQYFAQLKDLKSALVLSADLAKSTFTKIGENAFNDDYKNPYIKFGIREFAMAGAMNGISLHQGAKAIGGTFLAFSDYMKPAIRLTAISNLANLFIFSHDSYAVGGDGPTHQPVDQLPMLRAIPNVEVIRPADHYEVKHALSYSFKQKQKPICLVTSRQAIKQINEQKPQDFTKGAYIINSPFSFSENPDYTIIASGSEVSLANDAAKEIFEKHQLKVKVISAFNLNLFLQQKPEVIKNLVSSKNGLLAIEASSEMLWWKLSVYTDKFMQIAANQFGRSADGNKLMHEFGFSVENIINQLLNKK" }, "paralog": false, "identity": 0.9076576576576577, "score": 801.0, "evalue": 3.02e-293, "description": "Internal stop codon at 191011, 191239, 192157, 192160. Nonsense mutation around 191011, 191239, 192157, 192160.", "psc": { "uniref90_id": "UniRef90_Q9PPQ3", "gene": "TktA", "product": "transketolase", "ec_ids": [ "2.2.1.1" ], "uniref50_id": "UniRef50_A0A084EYA4", "cog_id": "COG0021", "cog_category": "G", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PPQ3", "EC:2.2.1.1", "UniRef:UniRef50_A0A084EYA4", "COG:COG0021", "COG:G" ] }, "pscc": { "uniref50_id": "UniRef50_A0A084EYA4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A084EYA4" ], "product": "Transketolase" } }, "id": "NKIECMJPJD_352", "locus": "GCA000828735_00317" }, { "type": "cds", "sequence": "contig_1", "start": 192309, "stop": 192818, "strand": "+", "frame": 3, "gene": null, "product": "Deoxyribose-phosphate aldolase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_W7YYY5" ], "nt": "ATGAATAAGTATAATATTTATGTAGATCACACATTATTAAAAGCTGACGCAAGTTTAGAAGAGATTCATAAGTTGTGTGAAGAAGCAGAAGAAAATGAATTTTATTCAGTTTGTATTAATCCTTGTTTTGTTAAAGTTGCAAAACACTATTTATTAGAAACGTCTGTTAAAATTTGCACAGTTATTGGTTTTCCACTAGGCGCTAACACAATTGAAACTAAAGTTTTTGAAACAAAAAACGCTATAGATTTAGGTGCTGATGAAATTGATATGGTTATTAATATTAATCAATTAATCAATGGTAATAGAGAATATTGCTTAAATGAAATTAATCAAATTAAAGAAGTTTGTGGGGATAAAATTTTAAAAGTGATCGTTGAAACAGCATTTTTGAACAGGGAGCAAAAAGAATTTGCAGCTAAAATTATTCTAGAATCAAATGCTGATTTCATTAAAACATCAACTGGTTTTGCAAAAGAAGGTGCAAAACTTGAAGATATTATTCTATGA", "aa": "MNKYNIYVDHTLLKADASLEEIHKLCEEAEENEFYSVCINPCFVKVAKHYLLETSVKICTVIGFPLGANTIETKVFETKNAIDLGADEIDMVININQLINGNREYCLNEINQIKEVCGDKILKVIVETAFLNREQKEFAAKIILESNADFIKTSTGFAKEGAKLEDIIL", "aa_hexdigest": "f1ea2795a7cd8f375ed13cf93750f6b6", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_A0A0M0LDG4", "query_cov": 0.9940828402366864, "subject_cov": 0.8, "identity": 0.506, "score": 165.0, "evalue": 1.33e-48, "valid": false, "gene": "deoC", "product": "deoxyribose-phosphate aldolase", "ec_ids": [ "4.1.2.4" ], "uniref50_id": "UniRef50_W7YYY5", "kegg_orthology_id": "K01619", "go_ids": [ "GO:0004139", "GO:0005737", "GO:0009264" ] }, "pscc": { "uniref50_id": "UniRef50_W7YYY5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_W7YYY5" ], "product": "Deoxyribose-phosphate aldolase" }, "genes": [], "id": "NKIECMJPJD_353", "locus": "GCA000828735_00318" }, { "type": "cds", "sequence": "contig_1", "start": 193105, "stop": 193512, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTCTTATCAAATTGGTTTTAAACAAAAAAAATTGTCTTTTTTGAATTTTAAAAAAATATTTGGCGATAAACAATTTATCAAGAAAGCATTAAAATTATTTTTTCCAGCGATTATCCAAAATATTATCATTACAGCAATTGCTTATGTTGATAATTTTTTTCTAGCAACATATACCCCTCAATCACAAGGATTAGATGCTAAAACAGCAACAATTCTTGCAACTCAATTATTATATTTACCAACAATTTTTACCGTTGCTGTTTGTTATGCATGCAGTGTTTATAGCTCACAATATTTAGGAAATAAAAATTATCATAAATTTAAAGAAACAACAAATTACATGATTTTAGGATCTGTTATATTAAATATCCCTTTTTTAATTATTTATTTAACTTTTCCACATTGA", "aa": "MSYQIGFKQKKLSFLNFKKIFGDKQFIKKALKLFFPAIIQNIIITAIAYVDNFFLATYTPQSQGLDAKTATILATQLLYLPTIFTVAVCYACSVYSSQYLGNKNYHKFKETTNYMILGSVILNIPFLIIYLTFPH", "aa_hexdigest": "4f69db167290c3fb03b5b36b224e129b", "start_type": "TTG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15477.16709999999, "isoelectric_point": 9.587367439270018 }, "id": "NKIECMJPJD_354", "locus": "GCA000828735_00319" }, { "type": "cds", "sequence": "contig_1", "start": 193618, "stop": 193911, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATTTACAATACGCAATGATTGGACAAGTTTTTTATGCTTTTTCATATCCTTTTGCAATTGCACACCGTCAAGATGCACGTCCAGCAATTCCTTTATTAGGAAGTATTGCCGCACTTTGTCTAAATTGTATTTTAGATCCAGTATTAATGATTTTAGTTGCCAAAACTCCTAAACAAGCGATATTACTTGCAGCATTCTCAACAATCGCTTCTCGTGGTTTTGATTTATTAATTGTTTTGCTTTGTGTTTGTATCTTTAAAAAAAGGGCAACATATGTTATTAATTATTGA", "aa": "MYLQYAMIGQVFYAFSYPFAIAHRQDARPAIPLLGSIAALCLNCILDPVLMILVAKTPKQAILLAAFSTIASRGFDLLIVLLCVCIFKKRATYVINY", "aa_hexdigest": "1de597eb850f6d65ab4f8edd5318f4ce", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10773.979799999985, "isoelectric_point": 9.236014747619631 }, "id": "NKIECMJPJD_355", "locus": "GCA000828735_00320" }, { "type": "cds", "sequence": "contig_1", "start": 195141, "stop": 195632, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCGCTTCGATTTTTTTAAAAAATCACAGAAAATAAAACCAAATTGGTTTATAAATCAATTTGGTGATAAAATTTTCATGAAAACAGCTGCTAAATTATTTTTCCCAGCAATTCTTGAAGTTATCATTTTATCGTCTGTTAATTATTTTGATAGTCTTTTCATTGCTTTATTTACACCTGAGAATATGGGGACAGCAGCCAAAACTGCAACCGTGTTAGCGACACAACTAATGTTTTTGCCAACTATTTTTTTCTATTCTATTGTAGCAGCAGCGGGGATTTTAGCCGCTCAATATTATGGTAAAAAAGACTATTTAAAATTTAAAGAAACAATTAACTTTATGTTGTTGTTTAGTTTTATTGTTGTAATGATATTTATTGTTATTTATATGGCAGCACCATTACAAATGATTCAATTATATTCAGGAAATAATTTAGATGTTTCAGCTTCAGAAGAAGCAAAACGAATTTATGATTTAACAAATCATTGA", "aa": "MRFDFFKKSQKIKPNWFINQFGDKIFMKTAAKLFFPAILEVIILSSVNYFDSLFIALFTPENMGTAAKTATVLATQLMFLPTIFFYSIVAAAGILAAQYYGKKDYLKFKETINFMLLFSFIVVMIFIVIYMAAPLQMIQLYSGNNLDVSASEEAKRIYDLTNH", "aa_hexdigest": "3a7fd3e2068eb2c358d45c8262c0dd3e", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18688.958399999992, "isoelectric_point": 9.222411918640137 }, "id": "NKIECMJPJD_356", "locus": "GCA000828735_00321" }, { "type": "cds", "sequence": "contig_1", "start": 195678, "stop": 195977, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTTCATTTTCATTAGCCACTGCTTATCGTCAACATAATAATGCAATCATGCCTTTAATTTCAAGTTCAATTGCAGTAACTGTTAATGTTATTTTAGATCCAATTTTGATTAAATATTGTGCCGTAACACCATTTGAGGCAATTTTATTTGTGGCTATTGCTACAATTGTTGCAAGATCTATTGATGCTTTAATAATGTTAATACTAACATTGTTTCGTAAACAATACCCTTATTATTTTTTAAATTATTTGAAATTAAGCAAAAAGGTCGTTAAATTAATTTTTATTCATGGTTGA", "aa": "MFSFSLATAYRQHNNAIMPLISSSIAVTVNVILDPILIKYCAVTPFEAILFVAIATIVARSIDALIMLILTLFRKQYPYYFLNYLKLSKKVVKLIFIHG", "aa_hexdigest": "6ef4b5f5595621ff1cdbfb3e228afd59", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11227.48399999999, "isoelectric_point": 9.722493171691895 }, "id": "NKIECMJPJD_357", "locus": "GCA000828735_00322" }, { "type": "cds", "sequence": "contig_1", "start": 196029, "stop": 196358, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTTATACAAGATATAGTCAAGATCATCGTGATGCTATTTCAACTGTTGCTTTAATTATTCAATTTACTAACTTAATTTGGCCAGGTGCCGCATCAACAGTTGCTGTTTTAGTGTTAGCTAATTTAGGTGCTAACAAGCATGATTTAGCTAAGGAAAACACGCGCAAATTAATTAATTGGGGTATGATAGTTGGAATTAGTTTGGGAATTATTTTGTTAGTTTTATCATCGTTTGTAAATAAATTATTAAATCCACCGTTGGATTATACAGAACAAGGTTTAAAACATGCAGCATACACTGCGACAATTGCTATGTATTTAGAATGA", "aa": "MFYTRYSQDHRDAISTVALIIQFTNLIWPGAASTVAVLVLANLGANKHDLAKENTRKLINWGMIVGISLGIILLVLSSFVNKLLNPPLDYTEQGLKHAAYTATIAMYLE", "aa_hexdigest": "2dbc44f607e10c7c8c8c7967e7d903a8", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12046.94219999999, "isoelectric_point": 8.04489688873291 }, "id": "NKIECMJPJD_358", "locus": "GCA000828735_00323" }, { "type": "cds", "sequence": "contig_1", "start": 196482, "stop": 196619, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGATGTATAACACATATAAATGTTCCAGAAGATTATAATGATCGTTTACATGTTGTATTATTATTTTTTATCATGGAATCCCAAAATATTTCTAAATTAATTATGGCTATTATTATCTTTAAAAAGACAAAATGA", "aa": "MGCITHINVPEDYNDRLHVVLLFFIMESQNISKLIMAIIIFKKTK", "aa_hexdigest": "d6574aee93cdbf872c4e332e0af903f2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5278.325099999997, "isoelectric_point": 7.944197273254395 }, "id": "NKIECMJPJD_359", "locus": "GCA000828735_00324" }, { "type": "cds", "sequence": "contig_1", "start": 196836, "stop": 197420, "strand": "+", "frame": 3, "gene": "srmB", "product": "ATP-dependent RNA helicase", "db_xrefs": [ "COG:COG0513", "COG:L", "SO:0001217", "UniRef:UniRef50_A0A9E2KV37", "UniRef:UniRef90_Q9PPQ7" ], "nt": "ATGCCATTAATCGCTAAAAGAGAAAATATTATTGGCGTTGCACCAACAGGGACAGGGAAAACTTTAGCCTTTGTTTTGCCTATATTAAACAACTTAGATTTATCTCAAAAACTGCAAGTTATAATTATTACTCCCACTCGTGAGTTAGCTCGCCAAATTTTTAGTAAAATTATTGTTTTTAAAAAACATCAACCACTTTTACAAGTTAAAATGTTAATTGGTGGTGAAAGTATTGACCAGCAAATTAATTCACAATTAAATAAAAGCCAACTGCTTATTGCAACTCCAACGCGTCTAAAACAAATTTTAACACGACAAATTCTTGATTTACACCATGTTAATGCACTTGTTTTTGATGAAGCTGATATGTTAATGGATCTTGGTTTTATTAGTGATTTAGAGTTTATTAACTCACAATTTACAAATAAAGATGTACAGAAAATAAGTTTTAGCGCTACACTGCACGAAATGTTATCGATCCAATTAAAAAAATATTTTAAAAATACAAAGATTATTAATATTAGTAATTCAATTTATGAAAATAAAAAAATAACCCATTATGTTATTCATAATCACGACAAATGA", "aa": "MPLIAKRENIIGVAPTGTGKTLAFVLPILNNLDLSQKLQVIIITPTRELARQIFSKIIVFKKHQPLLQVKMLIGGESIDQQINSQLNKSQLLIATPTRLKQILTRQILDLHHVNALVFDEADMLMDLGFISDLEFINSQFTNKDVQKISFSATLHEMLSIQLKKYFKNTKIINISNSIYENKKITHYVIHNHDK", "aa_hexdigest": "a7074c7a4c564365a804b81427c54d2d", "start_type": "ATG", "rbs_motif": null, "genes": [ "srmB" ], "truncated": "both", "pseudogene": { "start": 196746, "stop": 197723, "observations": { "mutation": [ 196773, 197418, 197679, 197703 ], "stop": [ 196773, 197418, 197679, 197703 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PPQ7", "query_cov": 1.0, "subject_cov": 0.43792325056433407, "identity": 1.0, "score": 367.0, "evalue": 1.44e-124, "gene_start": 1, "gene_end": 194, "reference_start": 31, "reference_end": 224, "reference_sequence": "MKFNSNYQKWILDSLINQKIFEPTPIQLKTMPLIAKRENIIGVAPTGTGKTLAFVLPILNNLDLSQKLQVIIITPTRELARQIFSKIIVFKKHQPLLQVKMLIGGESIDQQINSQLNKSQLLIATPTRLKQILTRQILDLHHVNALVFDEADMLMDLGFISDLEFINSQFTNKDVQKISFSATLHEMLSIQLKKYFKNTKIINISNSIYENKKITHYVIHNHDKWHSLSIIMKTIKPYLCIIFANTKKEVEAIYQYLIQQNKNVCILHGSLLQRERKNNYRDIKNNKFQYVVASDIASRGLDIDGVSHIINWNLPTENEWYIHRAGRSGRGKYSGESYVLYDQKEHDKLLNLSKKGIVFHHKFIKENELFDKEFLFKKKNQFKDLKTQNEIQKIISTTSTKIKPGYKKKIKEAIQKINQKNKRSHIEKKMNAHRIRGYKLKNS" }, "paralog": false, "identity": 0.9876923076923076, "score": 622.0, "evalue": 1.3e-227, "description": "Internal stop codon at 196773, 197418, 197679, 197703. Nonsense mutation around 196773, 197418, 197679, 197703.", "psc": { "uniref90_id": "UniRef90_Q9PPQ7", "gene": "SrmB", "product": "ATP-dependent RNA helicase", "uniref50_id": "UniRef50_A0A9E2KV37", "cog_id": "COG0513", "cog_category": "L", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PPQ7", "UniRef:UniRef50_A0A9E2KV37", "COG:COG0513", "COG:L" ] }, "pscc": { "uniref50_id": "UniRef50_A0A9E2KV37", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A9E2KV37" ], "product": "DEAD/DEAH box helicase" } }, "id": "NKIECMJPJD_360", "locus": "GCA000828735_00325" }, { "type": "cds", "sequence": "contig_1", "start": 197439, "stop": 197681, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00271.37" ], "nt": "ATGAAGACTATTAAACCTTATTTATGTATCATTTTTGCAAATACAAAAAAAGAAGTTGAAGCAATTTATCAATATTTAATTCAACAAAATAAAAATGTTTGCATTTTGCATGGCTCACTACTTCAACGTGAACGAAAAAACAACTATCGTGATATTAAAAATAATAAATTTCAGTATGTTGTTGCCTCAGATATTGCAAGTCGTGGTTTAGATATTGATGGTGTAAGTCATATTATTAATTGA", "aa": "MKTIKPYLCIIFANTKKEVEAIYQYLIQQNKNVCILHGSLLQRERKNNYRDIKNNKFQYVVASDIASRGLDIDGVSHIIN", "aa_hexdigest": "0ff7dee521859e5dd86b957ed6d6a2c4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00271.37", "name": "Helicase_C", "length": 74, "aa_cov": 0.9125, "hmm_cov": 0.9864864864864865, "evalue": 1.6495657658149458e-18, "score": 62.52600860595703, "start": 8, "stop": 80 } ], "seq_stats": { "molecular_weight": 9313.700799999995, "isoelectric_point": 9.39209270477295 }, "id": "NKIECMJPJD_361", "locus": "GCA000828735_00326" }, { "type": "cds", "sequence": "contig_1", "start": 197793, "stop": 198077, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATTTATCAAAAAAAGGAATCATCTTTCACCATAAATTCATTAAAGAAAATGAGTTATTTGATAAAGAATTTCTTTTTAAGAAAAAAAATCAATTTAAAGATTTAAAAACACAAAATGAAATTCAAAAAATAATCTCAACAACAAGTACAAAAATAAAACCAGGATATAAAAAGAAAATTAAAGAAGCTATTCAAAAAATTAATCAAAAAAACAAACGCTCTCATATCGAAAAGAAAATGAATGCTCACCGAATTCGTGGATATAAATTAAAAAATAGTTAA", "aa": "MNLSKKGIIFHHKFIKENELFDKEFLFKKKNQFKDLKTQNEIQKIISTTSTKIKPGYKKKIKEAIQKINQKNKRSHIEKKMNAHRIRGYKLKNS", "aa_hexdigest": "3318d57a64abded0b5a2224ba74861f8", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11315.27779999999, "isoelectric_point": 10.347127342224116 }, "id": "NKIECMJPJD_362", "locus": "GCA000828735_00327" }, { "type": "cds", "sequence": "contig_1", "start": 198564, "stop": 198980, "strand": "-", "frame": 1, "gene": "nusG", "product": "transcription termination/antitermination protein NusG", "db_xrefs": [ "COG:COG0250", "COG:K", "GO:0006355", "KEGG:K02601", "SO:0001217", "UniRef:UniRef50_Q9PPQ8", "UniRef:UniRef90_Q9PPQ8" ], "nt": "ATGACCGATCAAATTTGGTTTTTAATTCGTAATACCCAAATGGTTACTGGTTTAGTTGGTTCATCTGGAAAAAATGTTAAACCAATTCCTGTACCTGAAGATAAGATCTTAAAATTAATTGCTGATAATGATGCTAAACGTGCTTTAATAAGTCTAAATGAAAAAGTTGACCATGAAGAAAATATTGAAGTTATTGAATTGCATGAAGACGAAAATTTTACGAACTTTGAAGTTGATCAAGAAGTTAAAATTATCGCAGATACTTTTTTTGGTGAAATTGCAAAAATTGCAAAAATTGATCAAAACAAGAAAATTGCCACTGTAGAATTTGAATTTTTTGGCCGAATTAACACACTAGATTTAAATTTTAATGATATTCAACTTTATGATGAAGAAAGTGATAAGTTAGAAAACTAA", "aa": "MTDQIWFLIRNTQMVTGLVGSSGKNVKPIPVPEDKILKLIADNDAKRALISLNEKVDHEENIEVIELHEDENFTNFEVDQEVKIIADTFFGEIAKIAKIDQNKKIATVEFEFFGRINTLDLNFNDIQLYDEESDKLEN", "aa_hexdigest": "7074678074fa8ec5a9cd83f603a7318c", "start_type": "ATG", "rbs_motif": null, "genes": [ "nusG" ], "truncated": "3-prime", "pseudogene": { "start": 198564, "stop": 199280, "observations": { "mutation": [ 199076, 199265 ], "stop": [ 199076, 199265 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PPQ8", "query_cov": 1.0, "subject_cov": 0.5328185328185329, "identity": 0.9570000000000001, "score": 256.0, "evalue": 2e-84, "gene_start": 1, "gene_end": 138, "reference_start": 121, "reference_end": 258, "reference_sequence": "MAYKIKDLDSKLLSDLKIDFNHKHQWYIVTVVSGNEQKVIENIKDKLNGYGYGDKLSDLKIIKEKIKEVKIYEPSEAPRSMKNRANTKWETIVVDGATKYRCTKIKEGNKFNGYIFLKAEMTDQIWFLIRNTQMVTGLVGSSGKNVKPIPVPEDKILKLIADNDAKRALISLNEKVNHEENIEVIKMHEDENFVNFEVGQEVKIIADTFFGEIAKIAKIDQNKKIATVEFEFFGRINTLDLNFNDIQLYDEENDKLENQ" }, "paralog": false, "identity": 0.9621848739495799, "score": 456.0, "evalue": 1.02e-165, "description": "Internal stop codon at 199076, 199265. Nonsense mutation around 199076, 199265.", "psc": { "uniref90_id": "UniRef90_Q9PPQ8", "gene": "nusG", "product": "transcription termination/antitermination protein NusG", "uniref50_id": "UniRef50_Q9PPQ8", "cog_id": "COG0250", "cog_category": "K", "kegg_orthology_id": "K02601", "go_ids": [ "GO:0006355" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PPQ8", "UniRef:UniRef50_Q9PPQ8", "COG:COG0250", "COG:K", "KEGG:K02601", "GO:0006355" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PPQ8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PPQ8" ], "product": "Transcription termination/antitermination protein NusG" } }, "id": "NKIECMJPJD_363", "locus": "GCA000828735_00328" }, { "type": "cds", "sequence": "contig_1", "start": 199366, "stop": 199866, "strand": "-", "frame": 3, "gene": "secE", "product": "preprotein translocase subunit SecE", "db_xrefs": [ "COG:COG0690", "COG:U", "GO:0005886", "GO:0009306", "GO:0015450", "GO:0043952", "RefSeq:WP_006688437.1", "SO:0001217", "UniParc:UPI00000C1CCE", "UniRef:UniRef100_A0AAC9T2W1", "UniRef:UniRef50_A0AAC9T2W1", "UniRef:UniRef90_A0AAC9T2W1" ], "nt": "ATGGTTAATGACAAAAAACAAAAATCAAATAAATATTTAACGTACCAAGATCAAGAATATGAACGTAATCTTTTATTAGAAAAAGAACGTAAACAACAAAAAAAGATTTTGGCACAACAAGCATATCAACAATTAGATCTTAATAAAAAATTAATTAAAAAAGCTAAAAATGATCAAAAACTTATTTTAAGACTACTAAAAGAAGATAAATCAATTTCAAAAACACAATTTAATACCCACCGTTTAGAATTAAAAAATACAATTGATCAAATGGTTGATGAATATTATGAATTATTAGAACAATATAGTGTAGATTTTGAAAAAATAAGTTTTAAATTAAAACGTTGGCTCTATGGCATTAATAAAGAAATCAGACGTACTACTTGGGCTTCTAAACGTTCTGTTATTATCAGTTTAATCATTGTAATTGTTATTGTTTTAATTCTTGCTGCAATATTTTTTGGTATTGATACAGGTTTTTATAAATTATCAGCAAAATAA", "aa": "MVNDKKQKSNKYLTYQDQEYERNLLLEKERKQQKKILAQQAYQQLDLNKKLIKKAKNDQKLILRLLKEDKSISKTQFNTHRLELKNTIDQMVDEYYELLEQYSVDFEKISFKLKRWLYGINKEIRRTTWASKRSVIISLIIVIVIVLILAAIFFGIDTGFYKLSAK", "aa_hexdigest": "50e39382d02a9cb95353940a49210f12", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "ups": { "uniparc_id": "UPI00000C1CCE", "ncbi_nrp_id": "WP_006688437.1", "uniref100_id": "UniRef100_A0AAC9T2W1", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1CCE", "RefSeq:WP_006688437.1", "UniRef:UniRef100_A0AAC9T2W1" ] }, "ips": { "uniref100_id": "UniRef100_A0AAC9T2W1", "uniref90_id": "UniRef90_A0AAC9T2W1" }, "psc": { "uniref90_id": "UniRef90_A0AAC9T2W1", "gene": "secE", "product": "preprotein translocase subunit SecE", "uniref50_id": "UniRef50_A0AAC9T2W1", "cog_id": "COG0690", "cog_category": "U", "go_ids": [ "GO:0005886", "GO:0009306", "GO:0015450", "GO:0043952" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAC9T2W1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T2W1" ], "product": "Preprotein translocase subunit SecE" }, "genes": [ "secE" ], "id": "NKIECMJPJD_364", "locus": "GCA000828735_00329" }, { "type": "cds", "sequence": "contig_1", "start": 200417, "stop": 200608, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATAATTAGTATTCCATTAAATACAGACGCGTATATTGATTTATTATCTAATTATCCAATTGTTTTCTTTTTTGCAGTTTATGGTTTAATAATTGCTTTTGCCTTTTATAAAAAAGTTAAAACAAAGCAAAAAATAAAAGGCATTAAAAAACAATATTATACAAGTGATGTTACAAAACGTCCTAAATGA", "aa": "MIISIPLNTDAYIDLLSNYPIVFFFAVYGLIIAFAFYKKVKTKQKIKGIKKQYYTSDVTKRPK", "aa_hexdigest": "13c2585bf9d956af03058eb0a977b76d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7354.7341000000015, "isoelectric_point": 9.890175437927244 }, "id": "NKIECMJPJD_365", "locus": "GCA000828735_00330" }, { "type": "cds", "sequence": "contig_1", "start": 200705, "stop": 201523, "strand": "-", "frame": 2, "gene": "potE", "product": "APC family permease", "db_xrefs": [ "COG:COG0531", "COG:E", "SO:0001217", "UniRef:UniRef50_A0AAC9T1F8", "UniRef:UniRef90_A0AAC9T1F8" ], "nt": "GTGGTGGCAAGTTTAATCGCCTTTTTTACAGCATTAAGTTTTGCATATATTACCTTTTCTAAAAAATCTGGTTCTGGAATAGCTGGTATAATAGATGAATTAAAAGCTCCTAAATGTGCTCGTTTTATTTCTGTTTTGCAAACTTTTTTTTATAATGGAATACTGATGCCTTCCATTTCCTTTTTCGCTGCAGAATCATTATTAATGACAATAATACCTAAAAATTCATCATCACCACAAATATATCAAATTTTTATTTTGGCTATCGGGTTGTTTTTATTTTTTTTGTTGCTTAATTTTATTTCATTTAAATTTAGTTCAATTTTACAAAACATTGCAACAATTATTAAATTTATTCCTATTATTGCTATTGCTATTATTGGTATTACATATGGTATCAACAATGCACAAAATTCATTGTTCAATCATAATAATCCTAAGCATGCGAATTTTAGTCTTTTAGGAATATTAGCTTCTCTTCCTTCGATCTTATTCTCATTCGATTCTTTTTTAGGCATTTCATCATTACAACACAAAATTAAAAATGCTCAAAAAAATGTTCCAATTACATTAATTGTTGGTATGTTTTTAGTCGCTATTGTTTATATTCTAATAACTATAGGGCAAGTTTTTGTAGGCGAAGGTTATGCTTATGGTGTAATTAATAAAGTATTTAAAAATAATATCAATCTCCTAAAAATTATTACTATTATTGTTAATGTTTTTATTACAATTTCAATTATTGGTGTTTTAAATTCATTTGTTATTTTTTCAATTCATAATGCTCAATATGTAATTAATGAAAAAATTATTTTTTGA", "aa": "MVASLIAFFTALSFAYITFSKKSGSGIAGIIDELKAPKCARFISVLQTFFYNGILMPSISFFAAESLLMTIIPKNSSSPQIYQIFILAIGLFLFFLLLNFISFKFSSILQNIATIIKFIPIIAIAIIGITYGINNAQNSLFNHNNPKHANFSLLGILASLPSILFSFDSFLGISSLQHKIKNAQKNVPITLIVGMFLVAIVYILITIGQVFVGEGYAYGVINKVFKNNINLLKIITIIVNVFITISIIGVLNSFVIFSIHNAQYVINEKIIF", "aa_hexdigest": "89b0d22ace38cbb649d59c4e1f26c228", "start_type": "GTG", "rbs_motif": "4Base/6BMM", "genes": [ "potE" ], "truncated": "both", "pseudogene": { "start": 200402, "stop": 201676, "observations": { "mutation": [ 200419, 200623, 200698, 200704, 200707, 201526 ], "stop": [ 200419, 200623, 200698, 200704, 200707, 201526 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAC9T1F8", "query_cov": 1.0, "subject_cov": 0.5065176908752328, "identity": 0.996, "score": 491.0, "evalue": 1.03e-170, "gene_start": 1, "gene_end": 272, "reference_start": 52, "reference_end": 323, "reference_sequence": "MKISVKKTKQIGLFTSIAIMISSVVGIGIFFKNGSIFRFNNFNEIGIIISWVVASLIAFFTALSFAYITFSKKSGSGIAGIIDELKAPKCARFISVLQTFFYNGILMPSISFFAAESLLMTIIPKNSSSPQIYQIFILAIGLFLFFLLLNFISFKFSSILQNIATIIKFIPIIAIAIIGITYGINNAQNSLFNHNNPKHANFSLLGILASLPSILFSFDSFLGISSLQHKIKNAQKNVPITLIVGMFLVAIVYILITIGQVFVGEGYAYGVINKVFKNNINLLKIITIIVNVFITISIIGVLNSFVIFSIHNAQYVINEKIIFWWSWIQRVQSKHFKEAQGLISVLFIFFTWITIFMIISIPLNTDAYIDLLSNYPIVFFFAVYGLIIAFAFYKKVKTKQKIKGIKKQYYTSDVTKRPKWFFNYQNSSLKNKNILNKELKIANNWILSVWIIGITSCLFVFLYQFLYGYTIYAWLDPYNLKLYTFGLFYANNVSVIPWIVSLLFFVMLIFFIGFPFLNDAILNLNHKKVNKQQKVFV" }, "paralog": false, "identity": 0.9858490566037735, "score": 789.0, "evalue": 1.12e-290, "description": "Internal stop codon at 200419, 200623, 200698, 200704, 200707, 201526. Nonsense mutation around 200419, 200623, 200698, 200704, 200707, 201526.", "psc": { "uniref90_id": "UniRef90_A0AAC9T1F8", "gene": "PotE", "product": "APC family permease", "uniref50_id": "UniRef50_A0AAC9T1F8", "cog_id": "COG0531", "cog_category": "E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAC9T1F8", "UniRef:UniRef50_A0AAC9T1F8", "COG:COG0531", "COG:E" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAC9T1F8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T1F8" ], "product": "APC family permease" } }, "id": "NKIECMJPJD_366", "locus": "GCA000828735_00331" }, { "type": "cds", "sequence": "contig_1", "start": 201524, "stop": 201676, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGATTAGTGTTAAAAAAACAAAGCAAATAGGTTTGTTTACATCTATTGCAATTATGATTAGTAGCGTTGTGGGAATTGGTATTTTTTTTAAAAATGGATCTATTTTTCGGTTTAATAACTTTAATGAAATAGGAATTATTATTTCTTGA", "aa": "MKISVKKTKQIGLFTSIAIMISSVVGIGIFFKNGSIFRFNNFNEIGIIIS", "aa_hexdigest": "d02b8bfe4a7c48c4c31bf444b86e990b", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5523.598699999997, "isoelectric_point": 10.582501411437985 }, "id": "NKIECMJPJD_367", "locus": "GCA000828735_00332" }, { "type": "cds", "sequence": "contig_1", "start": 201903, "stop": 202073, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGTGTGCATGGCTTAATTAAAAATTCAGCTTTTAAAAAAATGATGGATGAAGGTAAACAAATTAAAAATTGGGCCTTTACTGACCGCAGATTTAGCGATGAAACACGCCAATATGTTAAAAAAGGTGAAGAAGATTTATATAGCCAAAACGCATCAATTCTTGGTTGA", "aa": "MGVHGLIKNSAFKKMMDEGKQIKNWAFTDRRFSDETRQYVKKGEEDLYSQNASILG", "aa_hexdigest": "8da63d32a9ac6e1b4a6abbca29aebad8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6498.299300000001, "isoelectric_point": 9.099341773986819 }, "id": "NKIECMJPJD_368", "locus": "GCA000828735_00333" }, { "type": "cds", "sequence": "contig_1", "start": 202125, "stop": 202274, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGGTTTAAAACCAGATTTATTAGTTAAAGGCGAACTATCAACTAGTGTCCATGAAGATCCTAACTTAGCTTATTATTTAAATGATGTAATAAAAAACCAATTAAGTGATATAGAACAAATTAGTATTAAAAAAAACCACATTAATTGA", "aa": "MGLKPDLLVKGELSTSVHEDPNLAYYLNDVIKNQLSDIEQISIKKNHIN", "aa_hexdigest": "3c502a750c01c94f61c18ce6699d151b", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5549.268999999999, "isoelectric_point": 5.425247764587401 }, "id": "NKIECMJPJD_369", "locus": "GCA000828735_00334" }, { "type": "cds", "sequence": "contig_1", "start": 202320, "stop": 202466, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAGCACAAGAAAAATTTAAAATTCCAACTTTAAAAAAATTACTAACAAATGATGAATTAGAATTGAAAAATGGTATACAAACGATTAAAACAGGCATTGATTCGCCATTTAAATATCATCGCGATCAAAACCGTGATGATTGA", "aa": "MKAQEKFKIPTLKKLLTNDELELKNGIQTIKTGIDSPFKYHRDQNRDD", "aa_hexdigest": "5707428e5a071329e54c8941694037e8", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5660.417800000003, "isoelectric_point": 9.09998645782471 }, "id": "NKIECMJPJD_370", "locus": "GCA000828735_00335" }, { "type": "cds", "sequence": "contig_1", "start": 202554, "stop": 202649, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTAACCAAAATTATAAAGGTTATTTAGCTCGTGAATTTTTAAAAATAAATCAAAAAAATGATTTAATTCCTTTACCAATGAGTATTGTTTGA", "aa": "MINQNYKGYLAREFLKINQKNDLIPLPMSIV", "aa_hexdigest": "3f0786630f4e02cd5089fe1566378f43", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3665.3290000000015, "isoelectric_point": 9.398732948303223 }, "id": "NKIECMJPJD_371", "locus": "GCA000828735_00336" }, { "type": "cds", "sequence": "contig_1", "start": 203055, "stop": 203159, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTGATAAAAATGAATATAAGTCAACTTTTAGTGCACAAATTGAAAATGTAATTAGGATCATTAAAGATGATATTAAAATGTATGCTAATTCACCATTTTGA", "aa": "MIDKNEYKSTFSAQIENVIRIIKDDIKMYANSPF", "aa_hexdigest": "95c4ffd2309b1de4357a211f45ec2d9f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4023.586800000001, "isoelectric_point": 6.000344657897949 }, "id": "NKIECMJPJD_372", "locus": "GCA000828735_00337" }, { "type": "cds", "sequence": "contig_1", "start": 203256, "stop": 203450, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAGAAAGAATAAAATAATAACATCATCATTAGCTTTATTGTTTACAGGAGTAATAGCAACGATAAGTATTGCCGCTTGCTCAAAACAAAATAATCAAAAAACAAAAAATTTTAATTCACAAGAACAAGCAAATAAGATTATTGAGGCTATATTAAATAAAAAAGAAGGTAAGGATAATTTTATCGATTGA", "aa": "MKRKNKIITSSLALLFTGVIATISIAACSKQNNQKTKNFNSQEQANKIIEAILNKKEGKDNFID", "aa_hexdigest": "8dbfb4fe54a2568f58744c09e76010b4", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7125.190599999999, "isoelectric_point": 9.845434379577636 }, "id": "NKIECMJPJD_373", "locus": "GCA000828735_00338" }, { "type": "cds", "sequence": "contig_1", "start": 204046, "stop": 204210, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTAGCAAATATTCAGATGAACTTATTCGTCGTTCAGTTAAAGGAATGCTTCCAAAAAATAAATTAGCAAAACAAATTCTAAACAAATTATTCATTTATAAAAATGATAAACATCCCCATGAAGCACAACAACCAACTATTTTGGAATTAAAATTAAAATAG", "aa": "MISKYSDELIRRSVKGMLPKNKLAKQILNKLFIYKNDKHPHEAQQPTILELKLK", "aa_hexdigest": "80fa0cefb490f4a33a84fd4059de7dc9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6389.581, "isoelectric_point": 9.997321891784669 }, "id": "NKIECMJPJD_374", "locus": "GCA000828735_00339" }, { "type": "cds", "sequence": "contig_1", "start": 204230, "stop": 204631, "strand": "+", "frame": 2, "gene": "rpsI", "product": "30S ribosomal protein S9", "db_xrefs": [ "COG:COG0103", "COG:J", "GO:0003723", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022627", "KEGG:K02996", "RefSeq:WP_006688496.1", "SO:0001217", "UniParc:UPI00001351DB", "UniRef:UniRef100_Q9PPR3", "UniRef:UniRef50_Q8R7Y9", "UniRef:UniRef90_Q9PPR3" ], "nt": "ATGCAAAAAAGTAATATTGTTGAATACAAAGGTTTAGGTCGTCGTAAATCTTCAATTGCAAGAGTTAAATTAGTTCCAGGTTCTGGAAAGGTTCTTGTTAATGATCGTCAACCAGAAAATTATTTTCCAAATAAATTAGTTATCCAAGACATGATGCAACCATTGGTTTTAACAAAAACAGCTGAAACTTATGATGTTTATGTTAAAGTTATTGGTGGTGGATTTAATGGACAAGCTGGAGCAATTCGTTTAGGAATTACTCGTGCTTTAATTCAAACTCGCGAAGATTTAAAAACCGATTTACGTAAAGCTGGTTTAGTAACACGTGATTCACGTGTTAAAGAACGTAAGAAATTTGGGCTATATGGTGCACGTCGTGCTCCTCAATTTACAAAACGTTAA", "aa": "MQKSNIVEYKGLGRRKSSIARVKLVPGSGKVLVNDRQPENYFPNKLVIQDMMQPLVLTKTAETYDVYVKVIGGGFNGQAGAIRLGITRALIQTREDLKTDLRKAGLVTRDSRVKERKKFGLYGARRAPQFTKR", "aa_hexdigest": "638aa3579e05ba8655ad30a37b265650", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "ups": { "uniparc_id": "UPI00001351DB", "ncbi_nrp_id": "WP_006688496.1", "uniref100_id": "UniRef100_Q9PPR3", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001351DB", "RefSeq:WP_006688496.1", "UniRef:UniRef100_Q9PPR3" ] }, "ips": { "uniref100_id": "UniRef100_Q9PPR3", "uniref90_id": "UniRef90_Q9PPR3" }, "psc": { "uniref90_id": "UniRef90_Q9PPR3", "gene": "rpsI", "product": "30S ribosomal protein S9", "uniref50_id": "UniRef50_Q8R7Y9", "cog_id": "COG0103", "cog_category": "J", "kegg_orthology_id": "K02996", "go_ids": [ "GO:0003723", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022627" ] }, "pscc": { "uniref50_id": "UniRef50_Q8R7Y9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q8R7Y9" ], "product": "Small ribosomal subunit protein uS9" }, "genes": [ "rpsI" ], "id": "NKIECMJPJD_375", "locus": "GCA000828735_00340" }, { "type": "cds", "sequence": "contig_1", "start": 204983, "stop": 205114, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTCCAATTGCTTTAGAAGCATTAAAAACAAATTTTAAAAAAGAATTTTTATTAGCAATTAATGGAATACAAGATGAAAATTATTTAAATAATTTATCTCAATTTAATTTTGAATTAATTTATAGTTGA", "aa": "MIPIALEALKTNFKKEFLLAINGIQDENYLNNLSQFNFELIYS", "aa_hexdigest": "f0d91f32b17a928bde30d37e1a1e2390", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5021.7370999999985, "isoelectric_point": 4.584314918518066 }, "id": "NKIECMJPJD_376", "locus": "GCA000828735_00341" }, { "type": "cds", "sequence": "contig_1", "start": 205319, "stop": 205546, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTAGTTAATTCTAATTCTGATTTGTTATTAAACACACAAACTAATCCTTATTTTATGGGATCACTTTTAATAAAAAAATCAATAAATAAAATAGTTGGATTTACATTAGTTTATACTCGTCCAAATATTGCAGAAATTCTTATTGAGCAAACAGATCGTGAAATTAGAGGTATGTATCAATATTTATTATCACAAAATTTAATAATTAATAATATTAATAATTGA", "aa": "MLVNSNSDLLLNTQTNPYFMGSLLIKKSINKIVGFTLVYTRPNIAEILIEQTDREIRGMYQYLLSQNLIINNINN", "aa_hexdigest": "956265830766ff660b4a2aaf89fbfad3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8647.950699999994, "isoelectric_point": 8.063592720031735 }, "id": "NKIECMJPJD_377", "locus": "GCA000828735_00342" }, { "type": "cds", "sequence": "contig_1", "start": 205660, "stop": 205758, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATAACTGATAAATTAATTAAAGAATTAAATGAAATTACAAGATTATATTATAAAAATGATCGTAATCAATTTAAAAAATCTTTAATAAATATATGA", "aa": "MITDKLIKELNEITRLYYKNDRNQFKKSLINI", "aa_hexdigest": "db9393899d8223c0d0dac12865769362", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3956.6103000000003, "isoelectric_point": 9.600390052795408 }, "id": "NKIECMJPJD_378", "locus": "GCA000828735_00343" }, { "type": "cds", "sequence": "contig_1", "start": 205768, "stop": 205890, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGTTATACTCCGAATTTGCAAGACCCAAAAACTTATAATGAAAAACTACTTTATATGTATTTAAATACAGATCAATCGAAAATTTTAGAAATTGTCGATAAAATAAGTTTTAAAAAATGA", "aa": "MGYTPNLQDPKTYNEKLLYMYLNTDQSKILEIVDKISFKK", "aa_hexdigest": "99bd04080f1ed1cb38e57b825e787b65", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4784.5039, "isoelectric_point": 7.982813835144044 }, "id": "NKIECMJPJD_379", "locus": "GCA000828735_00344" }, { "type": "cds", "sequence": "contig_1", "start": 206615, "stop": 207154, "strand": "-", "frame": 2, "gene": null, "product": "Potassium channel protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PPR6", "UniRef:UniRef90_Q9PPR6" ], "nt": "TTGCCTGGTAATCCGTTGTACAAACTTAAAAATTTTGGTGAAACTTTGTGGTTTTCTATGGGAACAATTACCACGATAGCTTATGGTGATGTTACACCTGTTTCTCCAGCTGGTAAAGTATTTGCCGTTATTTTAGGTGTAATTGGTGTTACCTATTATGGTTTTCTGACTTCATTATTTGCTTCAGCTTTAATTTCAGTAATTAATGAATTTGGTAAACGCCGTGATAGCCGTAGATTGTTAGAATTTCAAAATGAAAACGAGCGATTATTGCATGAATTTTCTGAGCAAATTAAAGTAGATATTGTCCAAGAATTAATTAAAAATAATGTTATTCAGGATCCAGAAGAAATTAAACGAATTGAAGATCTTCAAAATCAAGAACGTGAAGCTTTAAAAATGAAAGCGAAACGCCGAAAGAAAATTTATTTTAGTGATGATTTATTTGATGATACAAAACGAATTAGTGTTTCAAACGTCAAAGTACGTAGTGCTTCAAATGTAACAAAAGTATTAGGTCGATCATCAACAAAAAAATAA", "aa": "MPGNPLYKLKNFGETLWFSMGTITTIAYGDVTPVSPAGKVFAVILGVIGVTYYGFLTSLFASALISVINEFGKRRDSRRLLEFQNENERLLHEFSEQIKVDIVQELIKNNVIQDPEEIKRIEDLQNQEREALKMKAKRRKKIYFSDDLFDDTKRISVSNVKVRSASNVTKVLGRSSTKK", "aa_hexdigest": "9430f8e1a47fbf8add4c5d4005dee0b6", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 206615, "stop": 207454, "observations": { "mutation": [ 207259, 207280 ], "stop": [ 207259, 207280 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PPR6", "query_cov": 1.0, "subject_cov": 0.4182242990654206, "identity": 0.9390000000000001, "score": 315.0, "evalue": 9.83e-105, "gene_start": 1, "gene_end": 179, "reference_start": 250, "reference_end": 428, "reference_sequence": "MTNDERKEFIKKNKAIYFNRIRYFFYEWTDMKAYQRMFVPETFFSFFYQLLVIMIAFCSVTIIIYNHVELKQANEAHLDFIAKYFEVYYAFNIFFIIDYLIRISYADYTYNSISRLKAFLIAPFRFVNLLDLVSFVAIIIIYNTGLLDLITKMNEATSINEKISCYYQIVNNNAYMNIVPCMFLIKILGAHSKIIKNIILSSEVNFFWQIFRRRWRVLLSSFLILVLFTFLLSFIIYRIEQNYYAINNILPGNPLYKHKNFGETLWFAMGTITTIAYGDITPVSPAGKVFAVILGVIGVTYYGFLTSLFASALISVINEYGKRRDNRRFLEFQSENERLLHEFSEQIKVDIVQELIKNNVIQDPEEIKRIENLQNQEREALKIKAKRRKKIYFSDDLFDDTKRISVSNVKVRSASNVTKVLGRSSSKK" }, "paralog": false, "identity": 0.899641577060932, "score": 495.0, "evalue": 9.03e-178, "description": "Internal stop codon at 207259, 207280. Nonsense mutation around 207259, 207280.", "psc": { "uniref90_id": "UniRef90_Q9PPR6", "product": "Potassium channel protein", "uniref50_id": "UniRef50_Q9PPR6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PPR6", "UniRef:UniRef50_Q9PPR6" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PPR6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PPR6" ], "product": "Potassium channel protein" } }, "id": "NKIECMJPJD_380", "locus": "GCA000828735_00345" }, { "type": "cds", "sequence": "contig_1", "start": 207278, "stop": 207811, "strand": "-", "frame": 2, "gene": null, "product": "Potassium channel protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PPR6", "UniRef:UniRef90_Q9PPR6" ], "nt": "ATGAAAGCATATCAACGCATGTTAGTGCCTGAAACTTTTTTCTCATTTTTTTATCAATTATTCGTCTTAATGATTGCTTTTTGTTCCGTTACAATTATTATTTATAATCAAATTGAATTAAAACAAGCTAATGAAGAGCACATAGACTTTATTGCTAAATATTTTGAAGTTTATTATGCTTTTAATATTTTTTTTATTATTGATTATTTAATTCGTATTTCATACGCTGATTACACATACAATTCAATTTCAAGACTAAAGGCTTTTTTAATAGCACCATTTCGTTTTGTTAATTTATTGGATCTAGTATCTTTTGTAGCAATTGTTATCATTTATAATACAGGATTATTAGATTTTGTAGCAAAAATGAATGAAACAACTTCATATTCACAAAAATTTGATTATTATTATCAAATCATCAATAATAATGCTTATATGAATGTTGTTCCTTGTATGTTTTTAATTAAAATCTTAGGTGCGCATTCTAAAATTATCAAAAATGTTATTTTGAGTAGTGAAGTTAATTTCTTTTGA", "aa": "MKAYQRMLVPETFFSFFYQLFVLMIAFCSVTIIIYNQIELKQANEEHIDFIAKYFEVYYAFNIFFIIDYLIRISYADYTYNSISRLKAFLIAPFRFVNLLDLVSFVAIVIIYNTGLLDFVAKMNETTSYSQKFDYYYQIINNNAYMNVVPCMFLIKILGAHSKIIKNVILSSEVNFF", "aa_hexdigest": "d72716abb700c472aaa7d2922dd4329e", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 206975, "stop": 207901, "observations": { "mutation": [ 207259, 207280, 207820 ], "stop": [ 207259, 207280, 207820 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PPR6", "query_cov": 1.0, "subject_cov": 0.4135514018691589, "identity": 0.8809999999999999, "score": 300.0, "evalue": 7.22e-99, "gene_start": 1, "gene_end": 177, "reference_start": 31, "reference_end": 207, "reference_sequence": "MTNDERKEFIKKNKAIYFNRIRYFFYEWTDMKAYQRMFVPETFFSFFYQLLVIMIAFCSVTIIIYNHVELKQANEAHLDFIAKYFEVYYAFNIFFIIDYLIRISYADYTYNSISRLKAFLIAPFRFVNLLDLVSFVAIIIIYNTGLLDLITKMNEATSINEKISCYYQIVNNNAYMNIVPCMFLIKILGAHSKIIKNIILSSEVNFFWQIFRRRWRVLLSSFLILVLFTFLLSFIIYRIEQNYYAINNILPGNPLYKHKNFGETLWFAMGTITTIAYGDITPVSPAGKVFAVILGVIGVTYYGFLTSLFASALISVINEYGKRRDNRRFLEFQSENERLLHEFSEQIKVDIVQELIKNNVIQDPEEIKRIENLQNQEREALKIKAKRRKKIYFSDDLFDDTKRISVSNVKVRSASNVTKVLGRSSSKK" }, "paralog": false, "identity": 0.8928571428571429, "score": 555.0, "evalue": 1.15e-201, "description": "Internal stop codon at 207259, 207280, 207820. Nonsense mutation around 207259, 207280, 207820.", "psc": { "uniref90_id": "UniRef90_Q9PPR6", "product": "Potassium channel protein", "uniref50_id": "UniRef50_Q9PPR6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PPR6", "UniRef:UniRef50_Q9PPR6" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PPR6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PPR6" ], "product": "Potassium channel protein" } }, "id": "NKIECMJPJD_381", "locus": "GCA000828735_00346" }, { "type": "cds", "sequence": "contig_1", "start": 207966, "stop": 208145, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTGTTAAAATTATTGAAATTAAAGATAATGTTGATATCTTAAATGTATTAGATGAAGTCTTTGTTCGTGCTCAAAATGATGGTGTTAAAAATTTATTAATTTATGTTTTTAATGTAAAAATGTTATATTCATTAATAGATAATGAATACGAGTTGGTTAATGAGTCAATTATTTAA", "aa": "MFVKIIEIKDNVDILNVLDEVFVRAQNDGVKNLLIYVFNVKMLYSLIDNEYELVNESII", "aa_hexdigest": "a60f1c3eea219165c78953c432f4b640", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6920.004399999996, "isoelectric_point": 4.278464317321778 }, "id": "NKIECMJPJD_382", "locus": "GCA000828735_00347" }, { "type": "cds", "sequence": "contig_1", "start": 208185, "stop": 209453, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2C9DYI4", "UniRef:UniRef90_A0A2C9DYI4" ], "nt": "TTGCCTTCTAATAACCCTTTAATTAATTGGTGGAAAAGACGCCGTTTAAATACTGTTGTTAAAAAGATTGAAGAAATCAACTCAGCGTTCGCAGACCGTGAAATAACATCTCGTTCAAAAATCTTTTTGATGCCTGTTTTAACAGCATTTAGTACTTTAGGAACTACACTAGCTGTTCCTTTAATATCTGTTACTTTTTTACGGGGTGATGCAATTATTAATTTGTGGGGTAAAGATGGATATATTGCTTTCTTAAGTTTACTATTTTCTTTAGTTGTTGCTGTTATTATTTCAGCTTTTGTCGCTTTATTTAGTAGTTTAAAAAATAATAAACGTAATTATATTGTTTCAAACATGACCGAAGGTCTTAAAAGAATATATGATAAATATTTTATCGAAAGTAATTCTCAAGAAAAGATTAATGCTAAAGTAACTTTTTATTCACGCTTTTTAGAACGTTCTAAATTCGTTGTTCAAAATAATTACACATTTTTTTATGATGTTGTAGATATTAATAGTGATCAATATCCTAAAATATTAAAATATTTCAAAACCTTAAACCAATTAAATAATACAGTAATCTTCGATGCTAGTGGTTTTAAATATTTAGACGAACGTAAAATTTTCCGTAACATTATCGATTTAGAGAAAACAAATGTTGTCCGTTTGGATCGTTATAAAACAAAAACAAGCGGACGTCGCTTAATGAGTTTTATTTTCTATCAATTATCAATCATTGCTAATGTTAATACACGAAAACTTTTACAAAAATTTCCTTTCTTTGTCAACTCTTTATATCGTTTTTTAGATTACAGCGAAAAAAATACTGAGTTATTAACACTTTTATTAGACATTAAAAAACACGCTTCAAAAACAGAAATTCCATTAAACGATGAATCACAATTATTTTTTGTAGACTTTTTTACATTCGTGGTTTTTAAAGCTTTGGATGAATTAGGATTTGAGACGCTAATGAATGATTTAACAGTCTATGGTCGTCCATCTGAAATCACTAAGAAAAATATAACTTATAATTCATTAAAATTGGATTACATCATTAATCGTAATGCGCGTAATTTTGGACAACAAGCATTATTGTTTAACTTATTAGATTATTTTGATGAGACAGTAAATAAAAATATCTTTAACGAATTAGGAAATAGTAATAAAAACATGATTTTTTCTAAAAATCATCAATTAAATTTAGCCAATGAAGCTTTAAGTAAAAAAGGTTTTACAAAGCGTGAAATTGATTTAAATAATCATTGA", "aa": "MPSNNPLINWWKRRRLNTVVKKIEEINSAFADREITSRSKIFLMPVLTAFSTLGTTLAVPLISVTFLRGDAIINLWGKDGYIAFLSLLFSLVVAVIISAFVALFSSLKNNKRNYIVSNMTEGLKRIYDKYFIESNSQEKINAKVTFYSRFLERSKFVVQNNYTFFYDVVDINSDQYPKILKYFKTLNQLNNTVIFDASGFKYLDERKIFRNIIDLEKTNVVRLDRYKTKTSGRRLMSFIFYQLSIIANVNTRKLLQKFPFFVNSLYRFLDYSEKNTELLTLLLDIKKHASKTEIPLNDESQLFFVDFFTFVVFKALDELGFETLMNDLTVYGRPSEITKKNITYNSLKLDYIINRNARNFGQQALLFNLLDYFDETVNKNIFNELGNSNKNMIFSKNHQLNLANEALSKKGFTKREIDLNNH", "aa_hexdigest": "378f20328be78506d7d7f0b769dec39d", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 207966, "stop": 209702, "observations": { "mutation": [ 208187, 209546 ], "stop": [ 208187, 209546 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A2C9DYI4", "query_cov": 1.0, "subject_cov": 0.7301038062283737, "identity": 0.919, "score": 748.0, "evalue": 5.13e-269, "gene_start": 1, "gene_end": 422, "reference_start": 84, "reference_end": 505, "reference_sequence": "MRNNLSDLEIEKLTKTLVSKNKRVVALYGVPENASYKLMNDLISLMDVEIPNWSSISSATEDQCINDIKNFLLSNASLENFKNLPSNSPLINWWKKRRLNTVVKKIEEINSAFADREITSRSKIFLMPVLTAFSTLGTTLAVPLISVTFLRGDAIINLWGKSGYIAFLSLLFSLVIAVIISAFVALFSSLKNNKRNYIVSNMTEGFKRIYDKYFIDGNSEERINAKVTFYSRFLERSKLVVQNNYSFFYDVVDINSEQYPKMLKYFKTLNQLNNTVIFDASGFKYLDERKIFRNIIDPEKTNVVRLDRYKTKTSGRRLMNFIFYQLSIIANVNTRKLLQKFPFFVNSLYRFLDYSEKNTELLTLLLDLKKHASKTQVPLDDESQLFFVDFFTFVVFKALDESGFETLINDLTVYGRPSEITKKNITYNSLKLDYIINRNARNFGQQALLFNLLDYFDETGNKNIFNELGNSNKNMIFSKNHQLSLANEALSKKGFTKREIDLNAHWCDALYSNLHDDEDMFVKVIEIKENIDVLSALDEVFVRAQNENVKNLLIYVFNVKMLYCLIDNEYELVNESII" }, "paralog": false, "identity": 0.9204152249134948, "score": 1049.0, "evalue": 0.0, "description": "Internal stop codon at 208187, 209546. Nonsense mutation around 208187, 209546.", "psc": { "uniref90_id": "UniRef90_A0A2C9DYI4", "uniref50_id": "UniRef50_A0A2C9DYI4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A2C9DYI4", "UniRef:UniRef50_A0A2C9DYI4" ] }, "pscc": { "uniref50_id": "UniRef50_A0A2C9DYI4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2C9DYI4" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 49360.3677, "isoelectric_point": 9.620955467224118 }, "id": "NKIECMJPJD_383", "locus": "GCA000828735_00348" }, { "type": "cds", "sequence": "contig_1", "start": 209544, "stop": 209702, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGAAATAATTTATCTGATTTGGAAATCGAAAAATTGACCAAAACATTAGTATCAAAAAACAAAAGAGTTGTAGCTTTATATGGCGTTCCCGAAAACACTTCTTATAAACTAATGAACGATCTAATTAGTTTAATGGATGTAGAAATTCCTAATTGA", "aa": "MRNNLSDLEIEKLTKTLVSKNKRVVALYGVPENTSYKLMNDLISLMDVEIPN", "aa_hexdigest": "c917723a9b1a2d83135e1039ff7186e4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5951.885499999999, "isoelectric_point": 6.108509254455566 }, "id": "NKIECMJPJD_384", "locus": "GCA000828735_00349" }, { "type": "cds", "sequence": "contig_1", "start": 209898, "stop": 210317, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAGCGATTAAACAAAAATTGATGAATCTTGTTAATTTAAATCCGCATGATTCAATTATTTTAACAAGACTTTCATCATCAAGAAATATAGACTTATTTAAGTATATTAAAAAAGAAGATCTAATTAGTTTTGTTAATGGCGAAATTAGTTATGTTGAATTAAATGTAGGTAAGAATTTAAATTTATTTGAAGAAAGTTTAAAAAATGCTTTAAATATTGGTGAATTTTTAGAAATCTTAGTAAATTACAATGTAAATTTAGCAGATAATAAAATTAATTTACTACAACGTGATTTTCATGCAAATAAGAAAAAAATTATTCCTTTAGCAATTGAACAATTGTTAGCATTTAGAAAAAAGTTTATTTCTATTAATAGACTAGCAAAAAATTATTTTGAAGATACAACTGTTTGA", "aa": "MKKAIKQKLMNLVNLNPHDSIILTRLSSSRNIDLFKYIKKEDLISFVNGEISYVELNVGKNLNLFEESLKNALNIGEFLEILVNYNVNLADNKINLLQRDFHANKKKIIPLAIEQLLAFRKKFISINRLAKNYFEDTTV", "aa_hexdigest": "a8f62a4b4606102e00b10e683103dbdf", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16137.689499999988, "isoelectric_point": 9.521416282653806 }, "id": "NKIECMJPJD_385", "locus": "GCA000828735_00350" }, { "type": "cds", "sequence": "contig_1", "start": 210342, "stop": 211238, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13086.12" ], "nt": "TTGAAAGGAAAATTATTACCAAACGATCCTTTTAAATCACCATTAACTTTATTTAAAGTTGAAATTCGAGAAGAAGCTGATAAAATTTTTATCGCTAAAATTCAAGATGAGCCAATTGTTAATGAAAAAATCCAAATCTTTTTAAAAAAAGGTTATCAAGATTTTAAGGTTGAAACAACAGAATTGTTGACTAATTATGAATTGTCTTCAACTATTAAAATGATTGAAGATATGACAGGTCAAAAAATTAATATTAACGAAAACGAATTTGTTCCTTTCATGAACGAAAATGAGACACAAATTTTAGATCGATATACAACTTTTGAAATTGAACCATCAGCTGTACTGGGGTTATTCGAACCCGATGGGGGCGCTTTAAAAGCTGATTTACATAAAATTATTGAAATGGATGTTGATCCATTTGAATCAGAAAACGATGGTTTGAATAAACCAGTTGAATATTATGAAGATAAAGTAATTAAAGAACAAATGGTGGTGGAAATTGGACGTCCTCTAAATATTTTTCAAAAATATGCTGTTGCATCATCACTTTCACAAAATACTTTGATTTATGGACCTCCTGGAACAGGAAAGTCTGAAGTAATTGCAAATATTATCTTTAATGTTTTATTAAAAGGAAAAAGTAGTTTATTAGTATCAGAAAAACGTGCGGCTTTAGATGTATTAACAGAAAGAATAGGTTCGCTTTCACAATTTGCTTTATATGTTTATGATTTAACAAACAAAGAAACTTTTTTTGAAAAAATTTCTAATTTAAATGATTTATTAGGTACACAATGGTATCGTGAACAATCTCGTTCTACAAAGATTAAAGAAATAGAACCAATTAAATTTACTCCAGAGGAATCAATGTTTTTTAAAAATTATGAGGATTGA", "aa": "MKGKLLPNDPFKSPLTLFKVEIREEADKIFIAKIQDEPIVNEKIQIFLKKGYQDFKVETTELLTNYELSSTIKMIEDMTGQKININENEFVPFMNENETQILDRYTTFEIEPSAVLGLFEPDGGALKADLHKIIEMDVDPFESENDGLNKPVEYYEDKVIKEQMVVEIGRPLNIFQKYAVASSLSQNTLIYGPPGTGKSEVIANIIFNVLLKGKSSLLVSEKRAALDVLTERIGSLSQFALYVYDLTNKETFFEKISNLNDLLGTQWYREQSRSTKIKEIEPIKFTPEESMFFKNYED", "aa_hexdigest": "b8d9a627180b8df46607b0c5315c51b4", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13086.12", "name": "AAA_11", "length": 73, "aa_cov": 0.21476510067114093, "hmm_cov": 1.0, "evalue": 2.981087697083803e-09, "score": 32.567447662353516, "start": 172, "stop": 235 } ], "seq_stats": { "molecular_weight": 34229.70400000003, "isoelectric_point": 4.764665031433105 }, "id": "NKIECMJPJD_386", "locus": "GCA000828735_00351" }, { "type": "cds", "sequence": "contig_1", "start": 211455, "stop": 211889, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTAAAAATTGAAGATTTATTTACAGCTTATCTAAGATTTACTTCTTTTATTAAAAAATATAAGCTAACAGATAATTATAGTTCTATAGAAATTTTAAAACATTTAAAAATGATTACCAATAAAATTCAAACCAATGATGAATTAGTGACAAAATTTTTGATGCATGCTAATAGAATCACCAAAGACATTGATAATTATTACAACTTTTTAGCTGAACATAATCTTGAATCGAACACTATTTTTATGAATAAATCAATTCGTGATAAACGTATTTTTATAGACAAAATTGGTGATTATTTAAAGTTCCGTAAAGATGTGATAGATAAAGATTTTAGTTTGCAATCAAAAACAACACAAGAACTAAATGAAATTATTGATTTATGTGATAATTTTTTTACAAAACACAAAAAATTATTAGCCAAAAATGAATGA", "aa": "MLKIEDLFTAYLRFTSFIKKYKLTDNYSSIEILKHLKMITNKIQTNDELVTKFLMHANRITKDIDNYYNFLAEHNLESNTIFMNKSIRDKRIFIDKIGDYLKFRKDVIDKDFSLQSKTTQELNEIIDLCDNFFTKHKKLLAKNE", "aa_hexdigest": "e00d3d1fadb8de5d70b17bab52f6bc31", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17301.843999999994, "isoelectric_point": 9.025589942932129 }, "id": "NKIECMJPJD_387", "locus": "GCA000828735_00352" }, { "type": "cds", "sequence": "contig_1", "start": 212142, "stop": 212258, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGACCAAATTGCTTCGTATCGTGAATTTATCAACCAGGATGTTGATTTTTTGTCAAAATTACATACGCTTGCTGAAATTTATACACCAACAATGCAAGATATTATTCGTGAATGA", "aa": "MDQIASYREFINQDVDFLSKLHTLAEIYTPTMQDIIRE", "aa_hexdigest": "c344a0b52433001abc7b5573b21cf4a9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4546.0952, "isoelectric_point": 4.516164970397949 }, "id": "NKIECMJPJD_388", "locus": "GCA000828735_00353" }, { "type": "cds", "sequence": "contig_1", "start": 212430, "stop": 212630, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTTCTGAAACTAAAGGGCGTTTATTGCAAGATATTATTATTCAATTAAGACGTGAATCAATGCGCTCCGCTAAAATTGTTGGTGAAATTGTTTTTAAAAAATATATTAATAATTTAAGAAATTATTTAACAAAACTACCTCAACAAGAAAAAGATGAAATTACTAACGCATTACGAATTGCTTCATCACGTTCTTGA", "aa": "MFSETKGRLLQDIIIQLRRESMRSAKIVGEIVFKKYINNLRNYLTKLPQQEKDEITNALRIASSRS", "aa_hexdigest": "ba752e63339fe9bf8fc12ddd260f6ed0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7753.959999999998, "isoelectric_point": 10.197044944763181 }, "id": "NKIECMJPJD_389", "locus": "GCA000828735_00354" }, { "type": "cds", "sequence": "contig_1", "start": 212778, "stop": 212954, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAATTGAACGATCATATCCTATTGTGTATCGTTGTAAAACTAAAGTTGTCTCTGGTGATGATAAACAATTAAAGCCAACATCATTCTTTATTAATAAGTTAGCTGATAGTGACTTTGAAATTGATGATTTTGATAAAGTAGATTCTTTATTAGAACGTGCTAAAACTTCATGA", "aa": "MTIERSYPIVYRCKTKVVSGDDKQLKPTSFFINKLADSDFEIDDFDKVDSLLERAKTS", "aa_hexdigest": "1e2d5c71d24398821a42b045a0df4e72", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6705.5337, "isoelectric_point": 5.194254112243651 }, "id": "NKIECMJPJD_390", "locus": "GCA000828735_00355" }, { "type": "cds", "sequence": "contig_1", "start": 213246, "stop": 213710, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13087.12" ], "nt": "ATGAATATATTTGAAAAGGGATTGTGTGACAAAATTGAAAATAATGAAATTGTGATAACTAATCTTGAAAATGTTCAGGGTAACGAAGGTGATTTAGTTATTTTATCAATTGCTTATGGCCCAAATCCTGAAGGAAATTTACGTAATAATTTTGGACCACTTAATGCTAAGGGCGGAATGAATCGTTTAAATGTAGCGATCACTCGTGCACGTAAAAAAATGATTGTTATAAAATCACTTTATGGTCATCAAATTCAAGTTTCAAATCTTAATAATCAAAATGCACTGACATTTAAACGCTTTATTGAATATGTTGATCGAATTAATGGTCAATTAAGTATTAATGATTCACTTGAAAGTTTAGAACAACAAACATATTTAGAATTTGATAATGATTTAGTTAAAGAAATCTATAGCGAACTAACAAAAAAATTATCAAATAAATACCAAATCTTTCCAAATTGA", "aa": "MNIFEKGLCDKIENNEIVITNLENVQGNEGDLVILSIAYGPNPEGNLRNNFGPLNAKGGMNRLNVAITRARKKMIVIKSLYGHQIQVSNLNNQNALTFKRFIEYVDRINGQLSINDSLESLEQQTYLEFDNDLVKEIYSELTKKLSNKYQIFPN", "aa_hexdigest": "4c2d1db31369bcd890d2666c2f0eabc2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13087.12", "name": "AAA_12", "length": 65, "aa_cov": 0.42207792207792205, "hmm_cov": 0.8769230769230769, "evalue": 3.4765111607925624e-10, "score": 35.326683044433594, "start": 13, "stop": 77 } ], "seq_stats": { "molecular_weight": 17566.7526, "isoelectric_point": 5.8835973739624015 }, "id": "NKIECMJPJD_391", "locus": "GCA000828735_00356" }, { "type": "cds", "sequence": "contig_1", "start": 214241, "stop": 215866, "strand": "+", "frame": 2, "gene": "rnjA", "product": "Ribonuclease J", "db_xrefs": [ "COG:COG0595", "COG:J", "SO:0001217", "UniRef:UniRef50_A0A084EWI6", "UniRef:UniRef90_A0AAC9T1X3" ], "nt": "ATGGAAAATACCAAAAAAAGTCCTACATATGTTTATGCTTTAGGGGGGCTAGAAGAAATTGGTAAAAACACTTATGTTGTTGAACATGAAGATGAAATTATTTTAATTGATGCGGGCATTAAATTTGCTAATGCATCTTTACCAGGATTTGATGGAACTGTTGCCAATTTTGATTATTTAATAAAAAACAATCATAAAATTCATTCATTAGTAGTTACTCATGGTCATGAAGACCATATTGGGGGGATTCCTCACATTTTGCGACATGTTAAAATTAAGACTATCTATGCACCAACATTAGCAGCAAAATTAATTGAACGTCGTTTAAGTGAATATAAAGATATTAAACCTCCGCGTATTATTGTCTTTGAAGATGAGTCAATGTATAAAACAAAATATTTTGAAGTTGATTTTTATCGTGTTTGTCATTCAATTCCTGATTCATTTGGAATTTGTGTAAAAACACCAAACGGATACATTGTAACAACTGGTGATTTTAGATTTGATTTTGCTACAGCTGGTGATGAGACTAATTTAGCTAAGATTTCACAAATTTCTAATCGTGGAATCTCAGTATTAATGTGCGAATCAACAAGTGCTGAAATTCCTGGATTTAGTGAAAGTGAACGATATGTTATTGATAACATTCGTGATTATATGGTAAATATTAAAGGACGAACTTTTATTTCAACATTTGCTTCTAATTTAGGTCGTGTTGAAGAAATTATTGCTATAGCTGTTGGTTTAAATAAAAAAATCTGTATCATTGGTAAATCAATGGAAGCTAATATTAAAACAAGTCGAAAATTGGGTTATTTAAATGTTCCTGAATCTTCATTTATCACACATAAAGAATTGCCATTTTATAAAGATCATGAAATTGTTGTAATTTTAACTGGTTCACAAGGTGAAAAAATGGCGGCTTTAAATGTTATGGCTAATAATAACCACTCAAAAATTACTTTAAAACCATCAGATACAATTATCTTATCATCAAATCCAATCCCAGGAAACTATGCTCAAGTCGAAGCAATGGTTAATAAACTTTATAAACTCGGATTAACAGTTTATGAAAATTCACCTAACAAAAAGATTCATGCTTCTGGTCATGCCACACGGAGTGAACACCAATTAATGATTAAAGCAATTAATCCATCATATTTATTTCCAATCCATGGGGAATATAAAATGTTTCGTGCTTTAAAACAAAATGCTGTTGACCAAGGTTTTGATAAAGACCATGTCATTATTGCACCAAATGGCCAAAAATTACAATTATTAGATGGTGTTTTAAGTCATACTAATATTTATGTTGATGCTGAACCTAAATTTATTAATGGTTATGAAATTAGTTCTAAAATTAGTAAATTATTAAGTGAACGTGTTGTTTTATCCAGTGATGGTATTTTAAATCTAGTTTTAAATGCTGATTTTAAAAAAGCTAAGTTAAACTCTGCTGTAAGTATTTCAACACGAGGATGTTTTTTTGCCAAAGAATCAACTAATTTAATTAATAAAATTTCTAATGTAGCAAAATCAAGTTTAGAAGAAGCGTTGGCAAAAAAAGAATTTGATGAAAAAAAACTAAAAGAAATAGTTAGTGGAAATGTTAAATCAATTGTTTGA", "aa": "MENTKKSPTYVYALGGLEEIGKNTYVVEHEDEIILIDAGIKFANASLPGFDGTVANFDYLIKNNHKIHSLVVTHGHEDHIGGIPHILRHVKIKTIYAPTLAAKLIERRLSEYKDIKPPRIIVFEDESMYKTKYFEVDFYRVCHSIPDSFGICVKTPNGYIVTTGDFRFDFATAGDETNLAKISQISNRGISVLMCESTSAEIPGFSESERYVIDNIRDYMVNIKGRTFISTFASNLGRVEEIIAIAVGLNKKICIIGKSMEANIKTSRKLGYLNVPESSFITHKELPFYKDHEIVVILTGSQGEKMAALNVMANNNHSKITLKPSDTIILSSNPIPGNYAQVEAMVNKLYKLGLTVYENSPNKKIHASGHATRSEHQLMIKAINPSYLFPIHGEYKMFRALKQNAVDQGFDKDHVIIAPNGQKLQLLDGVLSHTNIYVDAEPKFINGYEISSKISKLLSERVVLSSDGILNLVLNADFKKAKLNSAVSISTRGCFFAKESTNLINKISNVAKSSLEEALAKKEFDEKKLKEIVSGNVKSIV", "aa_hexdigest": "3cb4053117f8a2a41cb7f02cf84fc5f9", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_A0AAC9T1X3", "query_cov": 1.0, "subject_cov": 0.9077181208053692, "identity": 0.98, "score": 1040.0, "evalue": 0.0, "valid": true, "gene": "RnjA", "product": "Ribonuclease J", "uniref50_id": "UniRef50_A0A084EWI6", "cog_id": "COG0595", "cog_category": "J" }, "pscc": { "uniref50_id": "UniRef50_A0A084EWI6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A084EWI6" ], "product": "Ribonuclease J" }, "genes": [ "rnjA" ], "id": "NKIECMJPJD_392", "locus": "GCA000828735_00357" }, { "type": "cds", "sequence": "contig_1", "start": 215918, "stop": 216031, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTCGAACAGTTTAGAAAAGACAATAATTATGTTGAATTTATCAAACAAGCTGAAGTTGAAGAAATTGAACAAGAAGTTGATATTGATGATTTAATTTCAAACGGTTTATAA", "aa": "MVEQFRKDNNYVEFIKQAEVEEIEQEVDIDDLISNGL", "aa_hexdigest": "ca3ef550d8d6e37edbd9c874352a9dad", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4400.781499999999, "isoelectric_point": 4.0500284194946286 }, "id": "NKIECMJPJD_393", "locus": "GCA000828735_00358" }, { "type": "cds", "sequence": "contig_1", "start": 216062, "stop": 216391, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08282.18" ], "nt": "ATGCAATATAAGATGTTAGTTATTGATCTAGATGGTACTTTATTAACGAAAACAAAAAACATTAGTAAAGCTAATTTACAAGGATTAAAAAAATATATTAGTTTAGGCGGTAAGGTTGTACTAAGTACAGGACGTTCATTAGAAAATACTTTAAAGATTGTGCATTTAATTCATGATCAAATCAAGGAATTAATCCAATATATTTCATGTTTTAATGGCTCATATATTTATGATGTTATTAATGAAAAAGTATTGTTTGAATCAACAATTAATAAAGATATAGTGAATGAAATCTATAAATTTTCATTAAAACATGGATTAGGATTTTGA", "aa": "MQYKMLVIDLDGTLLTKTKNISKANLQGLKKYISLGGKVVLSTGRSLENTLKIVHLIHDQIKELIQYISCFNGSYIYDVINEKVLFESTINKDIVNEIYKFSLKHGLGF", "aa_hexdigest": "190b62690c46d74f3f500d7179ea3241", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08282.18", "name": "Hydrolase_3", "length": 102, "aa_cov": 0.9357798165137615, "hmm_cov": 0.9411764705882353, "evalue": 3.766371368086365e-25, "score": 84.6131820678711, "start": 6, "stop": 107 } ], "seq_stats": { "molecular_weight": 12410.42999999999, "isoelectric_point": 9.1631010055542 }, "id": "NKIECMJPJD_394", "locus": "GCA000828735_00359" }, { "type": "cds", "sequence": "contig_1", "start": 216410, "stop": 216895, "strand": "+", "frame": 2, "gene": "cof", "product": "Cof-type HAD-IIB family hydrolase", "db_xrefs": [ "COG:COG0561", "COG:HR", "SO:0001217", "UniRef:UniRef50_A0AAC9T2T4", "UniRef:UniRef90_A0AAC9T2T4" ], "nt": "ATGCAAACACATTTTTTAGATGTTTATAATATTAACTATAAATTTTTATTACAACTTCATCACACAAAAAGAAAAGTTTGTTTAAACCCAATTTTTAATAAAAATGATAAATTTTATAAAATTAATATTTTACCTTCAAATTTTGTTAAAAAATTAAAACACGAGGTTATAGATCAATTGATTAAAGAATTTAGTGATCAAGTAAATATTTCATTCACCTCAAAATACATTGTTGAAGTGACTAATAAAAATATTAATAAGGCATCATCATTACGATTTATTGCTAATTTATACCAGATTAATTTAGATAAGGTTGCAACGATTGGCGATTCGCCTAATGATATTCCAATGTTTAAAATTAGTGGTTTAGCAGCGGCTGCTAGAACTAAATCTAAAACAATTATTGAACATGTTGATGTGATTATTAATTATAAAAATAATTCTAATTCTGTTGCATTATTTATTAATAATTACTTACTAAAATAA", "aa": "MQTHFLDVYNINYKFLLQLHHTKRKVCLNPIFNKNDKFYKINILPSNFVKKLKHEVIDQLIKEFSDQVNISFTSKYIVEVTNKNINKASSLRFIANLYQINLDKVATIGDSPNDIPMFKISGLAAAARTKSKTIIEHVDVIINYKNNSNSVALFINNYLLK", "aa_hexdigest": "79090405daa840d8e9cb59be89908c90", "start_type": "ATG", "rbs_motif": null, "genes": [ "cof" ], "truncated": "5-prime", "pseudogene": { "start": 216110, "stop": 216895, "observations": { "mutation": [ 216389 ], "stop": [ 216389 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAC9T2T4", "query_cov": 1.0, "subject_cov": 0.5812274368231047, "identity": 1.0, "score": 315.0, "evalue": 5e-107, "gene_start": 1, "gene_end": 161, "reference_start": 117, "reference_end": 277, "reference_sequence": "MQYKMLVIDLDGTLLTKTKNISKANLQGLKKYISLGGKVVLSTGRSLENTLKIVHLIHDQIKELIQYISCFNGSYIYDVINEKVLFESTINKDIVNEIYKFSLKHGLGFWPYNQRFMQTHFLDVYNINYKFLLQLHHTKRKVCLNPIFNKNDKFYKINILPSNFVKKLKHEVIDQLIKEFSDQVNISFTSKYIVEVTNKNINKASSLRFIANLYQINLDKVATIGDSPNDIPMFKISGLAAAARTKSKTIIEHVDVIINYKNNSNSVALFINNYLLK" }, "paralog": false, "identity": 0.9961685823754789, "score": 514.0, "evalue": 3.46e-188, "description": "Internal stop codon at 216389. Nonsense mutation around 216389.", "psc": { "uniref90_id": "UniRef90_A0AAC9T2T4", "gene": "Cof", "product": "Cof-type HAD-IIB family hydrolase", "uniref50_id": "UniRef50_A0AAC9T2T4", "cog_id": "COG0561", "cog_category": "HR", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAC9T2T4", "UniRef:UniRef50_A0AAC9T2T4", "COG:COG0561", "COG:HR" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAC9T2T4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T2T4" ], "product": "Cof-type HAD-IIB family hydrolase" } }, "id": "NKIECMJPJD_395", "locus": "GCA000828735_00360" }, { "type": "cds", "sequence": "contig_1", "start": 216991, "stop": 217221, "strand": "+", "frame": 1, "gene": null, "product": "Small ribosomal subunit protein bS16", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A2RJS1" ], "nt": "TTGGTAAAGATTAGATTAACAAGAGTAGGAACACATAAAAAACCATTTTTTCGTATTGTAGTTATGGATGCTAAAACCAAAGCTAATGGTGCATATATTGAAAATTTAGGTCATTATGATCCTGTTTTAGGACAAGTTGTTTTAAAAAAAGAAGCTATTTTAGCTCAATTACAAAACGGAGCACAACCTAGTGAAACGGTAAAAAATATCTTATCACAAGAAGGTATTTGA", "aa": "MVKIRLTRVGTHKKPFFRIVVMDAKTKANGAYIENLGHYDPVLGQVVLKKEAILAQLQNGAQPSETVKNILSQEGI", "aa_hexdigest": "b2e9518415e3cee2360d06483aea9a4b", "start_type": "TTG", "rbs_motif": "GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_A0A841CAP4", "query_cov": 1.013157894736842, "subject_cov": 0.8461538461538461, "identity": 0.5579999999999999, "score": 89.0, "evalue": 1.29e-21, "valid": false, "gene": "rpsP", "product": "30S ribosomal protein S16", "uniref50_id": "UniRef50_A2RJS1", "go_ids": [ "GO:0000314", "GO:0003735", "GO:0006412", "GO:0022627" ] }, "pscc": { "uniref50_id": "UniRef50_A2RJS1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A2RJS1" ], "product": "Small ribosomal subunit protein bS16" }, "genes": [], "id": "NKIECMJPJD_396", "locus": "GCA000828735_00361" }, { "type": "cds", "sequence": "contig_1", "start": 217300, "stop": 217986, "strand": "+", "frame": 1, "gene": "trmD", "product": "tRNA (guanosine(37)-N1)-methyltransferase TrmD", "db_xrefs": [ "COG:COG0336", "COG:J", "EC:2.1.1.228", "GO:0002939", "GO:0005829", "GO:0030488", "GO:0052906", "KEGG:K00554", "RefSeq:WP_006688650.1", "SO:0001217", "UniParc:UPI0000137433", "UniRef:UniRef100_Q9PPS2", "UniRef:UniRef50_Q6F0S5", "UniRef:UniRef90_Q9PPS2" ], "nt": "ATGAAAATATCGATTTTATCTTTATTTCCTGAATTATATGAAACATGGATAAAGCATTCAATTATTAGTAATGCTATTAAAAATAACCAAGTTATAATAGAAGTTATCGATTTTCGTTTATATACAAATGATAAACATAAAAAAGTTGATGATTACCAATATGGTGGCGGAGCAGGTATGGTTTTAAGGATTGAACCAATTGTTGCTGCCATTCGTGCAATTCGTTCTTCTAATTCATATGTGATTTTAACAACACCAAAAGGTCAAGTTTTTAATCAAGAATTAGCAAACAATTTTGTTAGTAAATATGATCATATCATCATTATTGCTGGTCATTATGAAGGTTTTGATGAACGTATTAATTATTACATTGACGCCCAATATAGTATCGGTGATTTTGTCTTAACAGGGGGGGAATTACCAAGTATGGTAATTTCTGATGCTGTTATTCGTTTGTTAGATGGCGTTATTTCTCCTTCTAGTTTAGAAACTGAATCATTTAATAACTACTTATTAGATTATCCTGTTTACACACGCCCTCTTGTTTTTGAAGGGCATAAAGTACCGGATATACTATTATCTGGTCATCATAAAAATATTGCTGATTTTCGTAAACAGCAACAAGAGACGATTACTAAAAAGAATCGTCCTGATTTATATCAAAAATATTTAAATAACAAAAAGTAG", "aa": "MKISILSLFPELYETWIKHSIISNAIKNNQVIIEVIDFRLYTNDKHKKVDDYQYGGGAGMVLRIEPIVAAIRAIRSSNSYVILTTPKGQVFNQELANNFVSKYDHIIIIAGHYEGFDERINYYIDAQYSIGDFVLTGGELPSMVISDAVIRLLDGVISPSSLETESFNNYLLDYPVYTRPLVFEGHKVPDILLSGHHKNIADFRKQQQETITKKNRPDLYQKYLNNKK", "aa_hexdigest": "3fd9c8fd73f843c65de0b600f7534a25", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000137433", "ncbi_nrp_id": "WP_006688650.1", "uniref100_id": "UniRef100_Q9PPS2", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000137433", "RefSeq:WP_006688650.1", "UniRef:UniRef100_Q9PPS2" ] }, "ips": { "uniref100_id": "UniRef100_Q9PPS2", "uniref90_id": "UniRef90_Q9PPS2" }, "psc": { "uniref90_id": "UniRef90_Q9PPS2", "gene": "trmD", "product": "tRNA (guanosine(37)-N1)-methyltransferase TrmD", "ec_ids": [ "2.1.1.228" ], "uniref50_id": "UniRef50_Q6F0S5", "cog_id": "COG0336", "cog_category": "J", "kegg_orthology_id": "K00554", "go_ids": [ "GO:0002939", "GO:0005829", "GO:0030488", "GO:0052906" ] }, "pscc": { "uniref50_id": "UniRef50_Q6F0S5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q6F0S5" ], "product": "tRNA (guanine-N(1)-)-methyltransferase" }, "genes": [ "trmD" ], "id": "NKIECMJPJD_397", "locus": "GCA000828735_00362" }, { "type": "cds", "sequence": "contig_1", "start": 217996, "stop": 218367, "strand": "+", "frame": 1, "gene": "rplS", "product": "50S ribosomal protein L19", "db_xrefs": [ "GO:0000311", "GO:0003735", "GO:0006412", "GO:0022625", "RefSeq:WP_006688591.1", "SO:0001217", "UniParc:UPI0000133CCE", "UniRef:UniRef100_Q9PPS3", "UniRef:UniRef50_Q8EWU9", "UniRef:UniRef90_Q9PPS3" ], "nt": "ATGGCTTTATTTAAGATTAACAAAGGCGAAATTATGAATTTTGTTAATTCAACACAATTAAAAACAGATATTCCTAATTTTGATTCTGGTGATACAATTATCGTTCATAATCGAATTGTAGAAGGAAAAAAATCACGAATTCAAAAGTTTGAAGGCGTTGTTTTACGTCGTCGTGGTTCTGGTTCAAGTGAAACAGTTATTGTTCGTAAAGAATCAAGTGGTATAGGTGTTGAACAATCATTCAATATTCATTCTCCACTTGTTGAAAAAATTGAAGTTATCAAGTATGGTAAAGTAAGACGTGCTTATATTTCATACATGCGTAATCGTTCAGGCAAATCTGCAAGAATTAAAGAATTAAATAAACAATAA", "aa": "MALFKINKGEIMNFVNSTQLKTDIPNFDSGDTIIVHNRIVEGKKSRIQKFEGVVLRRRGSGSSETVIVRKESSGIGVEQSFNIHSPLVEKIEVIKYGKVRRAYISYMRNRSGKSARIKELNKQ", "aa_hexdigest": "134ea9784a49d661660ac92647524c7f", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "ups": { "uniparc_id": "UPI0000133CCE", "ncbi_nrp_id": "WP_006688591.1", "uniref100_id": "UniRef100_Q9PPS3", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000133CCE", "RefSeq:WP_006688591.1", "UniRef:UniRef100_Q9PPS3" ] }, "ips": { "uniref100_id": "UniRef100_Q9PPS3", "uniref90_id": "UniRef90_Q9PPS3" }, "psc": { "uniref90_id": "UniRef90_Q9PPS3", "gene": "rplS", "product": "50S ribosomal protein L19", "uniref50_id": "UniRef50_Q8EWU9", "go_ids": [ "GO:0000311", "GO:0003735", "GO:0006412", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_Q8EWU9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q8EWU9" ], "product": "Large ribosomal subunit protein bL19" }, "genes": [ "rplS" ], "id": "NKIECMJPJD_398", "locus": "GCA000828735_00363" }, { "type": "cds", "sequence": "contig_1", "start": 218811, "stop": 218912, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGAACATCACAAGGGATCGCACGAATTTTACAATCAATTATGATTTCAAATCATAGTTCACCAAAAGAAGCCAAATTAGCATACGATATTATGTTTTGA", "aa": "MGTSQGIARILQSIMISNHSSPKEAKLAYDIMF", "aa_hexdigest": "512c2bd4f28003a02a85091d37342755", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3639.2273999999998, "isoelectric_point": 8.275113487243654 }, "id": "NKIECMJPJD_399", "locus": "GCA000828735_00364" }, { "type": "cds", "sequence": "contig_1", "start": 218985, "stop": 219359, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACATTGACATATATTATTACTGTGCAAGTTTTTGGTTTTGCTTTAAGTCAAATTCCAAACGTAGAAAAAATTATGATTTTTGGCGACAACCGTTTAAGTTATCCACCTATTAATTTGTTTTTTACTAATGGTATTTTAGATGAAAAAATAGTTAAAAATATTGAAACTGGTGTCTTGAATTATTCTAATATTACTAATTCAAAAATTGCTGATGAATTTATGCATTATGTACAAGCAAATCCTAATGCGACAATTGGTGATTTATTTCATTTAAACCAAAGTTTGACTTATCGACAATCTTTGTATGAATTTGCTAAGCCCTTATATATTTTTCATGATCTTATTATTAATAAAGTACAAATTTTATCTTGA", "aa": "MTLTYIITVQVFGFALSQIPNVEKIMIFGDNRLSYPPINLFFTNGILDEKIVKNIETGVLNYSNITNSKIADEFMHYVQANPNATIGDLFHLNQSLTYRQSLYEFAKPLYIFHDLIINKVQILS", "aa_hexdigest": "52cccbf367c31b3ddc46bcdd5d7939a2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14250.298099999982, "isoelectric_point": 5.821529197692871 }, "id": "NKIECMJPJD_400", "locus": "GCA000828735_00365" }, { "type": "cds", "sequence": "contig_1", "start": 219648, "stop": 219986, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTAGCATCAATTCTTGCTTCAATTGTTTTAGGAATTGTTTTTAACATTTATTTTCCAAAACATAAAAGTTTGAAAATTCAAGTTTATTCAAAAAACGTTAATGCTATCATTGAAAATTTACGTGCTAATGATTTTAATAATTCAATTACTTTAAATAGTTTAAGTGATTCATTAACATTAAGAACAAATTATTCATTAGAAATTATTTCTCCATATATTGAGTTACCATCTTTAATTCATTTAGTGCGCGATATTGATAAGAATTGTTTAATAGTGATCTATCCAATTATTGATATTGATGGAGAAATGGTTGTTAAGAAATCTACCATTAGTTAA", "aa": "MVASILASIVLGIVFNIYFPKHKSLKIQVYSKNVNAIIENLRANDFNNSITLNSLSDSLTLRTNYSLEIISPYIELPSLIHLVRDIDKNCLIVIYPIIDIDGEMVVKKSTIS", "aa_hexdigest": "06f95f2e3122e8e17d355b794d00fc68", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12593.632699999987, "isoelectric_point": 6.709467887878417 }, "id": "NKIECMJPJD_401", "locus": "GCA000828735_00366" }, { "type": "cds", "sequence": "contig_1", "start": 220174, "stop": 220326, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T1F4" ], "nt": "ATGATTAATTTAAATAATAATCAAAATAAATTTTTTAATCATACCATTCGCATTTTTTATGGCAAACGTATTTTTTGCTCTCGTTTTATTATTATTTGCATTTCTATTATTTATAAGATGTATATTAATACAATTAAAATTAATAAGCGTTGA", "aa": "MINLNNNQNKFFNHTIRIFYGKRIFCSRFIIICISIIYKMYINTIKINKR", "aa_hexdigest": "025c6fa9b00d1c2804fe2c2beb7196b7", "start_type": "ATG", "rbs_motif": "AGGAG/GGAGG", "psc": { "uniref90_id": "UniRef90_A0AAC9T1F4", "query_cov": 1.1, "subject_cov": 0.859375, "identity": 0.818, "score": 82.8, "evalue": 5.86e-20, "valid": false, "uniref50_id": "UniRef50_A0AAC9T1F4" }, "pscc": { "uniref50_id": "UniRef50_A0AAC9T1F4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T1F4" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6173.395399999998, "isoelectric_point": 10.463815116882323 }, "id": "NKIECMJPJD_402", "locus": "GCA000828735_00367" }, { "type": "cds", "sequence": "contig_1", "start": 220416, "stop": 220970, "strand": "+", "frame": 3, "gene": null, "product": "Iron ABC transporter substrate-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T094", "UniRef:UniRef90_A0AAC9T094" ], "nt": "ATGAAAACAAAAAGTAATCTCAAAGCAAGATTAATAATCTTTATTAGTATATTATTAACAACTATTATTAGTTTAGTTTTATTAACTAGTTGTTCAACAAAAAAATCTAAAAAAGTAACAATTAATCAAGATACGCAAATCAAGTTTCAATCAATCACAAATAATAATATTAATCTTGCAAATGAAAAACAAATATTAAATCAATCTCAAAATAAAGAAACTAAAATTTTTCTAAACCTCTATGAGAGTTTAAAAGATTATTTTGATCAAACAATTAAACGCTCCTTGTATTTATATTATTCTTATAATTTTGCTTTACTAAATTTATATAATGCAAAATATCATGTTAAGTTAAATGATTTTAGTTTAATTTACAATACAGATAAAAATTCAAATAATGGTACTTTTGATATTAATTATAAATTAGGCTTAGTTATTGATGACATTGGTGATATTAATATACAAACTAAACCTCAATTTTATGAGCCAATTCCACCTGACAAAAAAGTGAGAAATGATGATTATCACTTTACTAAGAAAATTAAACGTTTTTGA", "aa": "MKTKSNLKARLIIFISILLTTIISLVLLTSCSTKKSKKVTINQDTQIKFQSITNNNINLANEKQILNQSQNKETKIFLNLYESLKDYFDQTIKRSLYLYYSYNFALLNLYNAKYHVKLNDFSLIYNTDKNSNNGTFDINYKLGLVIDDIGDINIQTKPQFYEPIPPDKKVRNDDYHFTKKIKRF", "aa_hexdigest": "9ebc434f305245b388680679887dae9b", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 220416, "stop": 221273, "observations": { "mutation": [ 220968, 221127 ], "stop": [ 220968, 221127 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAC9T094", "query_cov": 1.0, "subject_cov": 0.4634760705289673, "identity": 1.0, "score": 347.0, "evalue": 1.68e-117, "gene_start": 1, "gene_end": 184, "reference_start": 1, "reference_end": 184, "reference_sequence": "MKTKSNLKARLIIFISILLTTIISLVLLTSCSTKKSKKVTINQDTQIKFQSITNNNINLANEKQILNQSQNKETKIFLNLYESLKDYFDQTIKRSLYLYYSYNFALLNLYNAKYHVKLNDFSLIYNTDKNSNNGTFDINYKLGLVIDDIGDINIQTKPQFYEPIPPDKKVRNDDYHFTKKIKRFWFGSKKAIIDYFNEAISMQINYSNVKIYHSIIKYPDSKEHYEGYTITGGSLYRWVKQAIPTDDNQNKVRYMISKINDLNIDNDVKLFNLNDLVLKQIFIVDRPAQTSIKPERDLVYTNIWNNKLDDFYFSPIAFLAPTFIKKNGKKITPFNLKTNKEYLDNLYENLLIQGQKNNNALIKGMYVIYSMYSYFDLTLEPKKPGSIHHCHADGFCH" }, "paralog": false, "identity": 0.9929824561403509, "score": 562.0, "evalue": 1.28e-204, "description": "Internal stop codon at 220968, 221127. Nonsense mutation around 220968, 221127.", "psc": { "uniref90_id": "UniRef90_A0AAC9T094", "product": "Iron ABC transporter substrate-binding protein", "uniref50_id": "UniRef50_A0AAC9T094", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAC9T094", "UniRef:UniRef50_A0AAC9T094" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAC9T094", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T094" ], "product": "Iron ABC transporter substrate-binding protein" } }, "id": "NKIECMJPJD_403", "locus": "GCA000828735_00368" }, { "type": "cds", "sequence": "contig_1", "start": 221019, "stop": 221129, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAATTAATTATTCAAATGTCAAAATTTATCATTCGATTATTAAATATCCAGATAGCAAAGAACATTATGAAGGATATACAATAACTGGTGGTAGTTTATATCGTTGA", "aa": "MQINYSNVKIYHSIIKYPDSKEHYEGYTITGGSLYR", "aa_hexdigest": "71098ad6f2a02ec3f98db2fc0c9d6864", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4269.744200000001, "isoelectric_point": 8.048571586608887 }, "id": "NKIECMJPJD_404", "locus": "GCA000828735_00369" }, { "type": "cds", "sequence": "contig_1", "start": 221178, "stop": 221327, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTAGTAAAATTAATGATTTAAATATTGATAACGATGTTAAATTATTTAATTTAAATGATCTTGTTTTAAAACAAATTTTTATTGTTGATCGTCCTGCACAAACATCTATTAAACCCGAACGTGATCTTGTTTATACCAATATTTGA", "aa": "MISKINDLNIDNDVKLFNLNDLVLKQIFIVDRPAQTSIKPERDLVYTNI", "aa_hexdigest": "6040900ddc5eb75f043937c51dec7d0a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5701.548600000001, "isoelectric_point": 4.919437599182127 }, "id": "NKIECMJPJD_405", "locus": "GCA000828735_00370" }, { "type": "cds", "sequence": "contig_1", "start": 221508, "stop": 221609, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATGTTATTTATAGTATGTATAGTTATTTTGATTTAACACTTGAACCTAAAAAACCAGGTAGTATTCATCATTGCCATGCTGATGGTTTTTGTCATTAA", "aa": "MYVIYSMYSYFDLTLEPKKPGSIHHCHADGFCH", "aa_hexdigest": "a805e6a5fb9387a637bf1d75611b968e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3891.4321999999993, "isoelectric_point": 6.363432121276855 }, "id": "NKIECMJPJD_406", "locus": "GCA000828735_00371" }, { "type": "cds", "sequence": "contig_1", "start": 221644, "stop": 221865, "strand": "+", "frame": 1, "gene": null, "product": "DUF3899 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9X6J3", "UniRef:UniRef90_A0AAC9X6J3" ], "nt": "ATGAGTATAAAAAAACAATCGTTTCCGAATCGTTTATTAAATTATAAAAGCATTATTGTTTTAATTGCATTATTAATATTGATGGTTATTAGTCACTTAATTGTTGAATTAATTAAAGATTATCACCAATACCGTATGAATGTTTTTATAGCTTATACATGTATCTTTATGGTAGTTTTCTTATATATTATCTTCTCGCGCCAAGTTTATCGTCGCCGTTGA", "aa": "MSIKKQSFPNRLLNYKSIIVLIALLILMVISHLIVELIKDYHQYRMNVFIAYTCIFMVVFLYIIFSRQVYRRR", "aa_hexdigest": "5c5d7163bb41b6b7885bc2d2902f4ac0", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 221644, "stop": 222084, "observations": { "mutation": [ 221863, 221908 ], "stop": [ 221863, 221908 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAC9X6J3", "query_cov": 1.0, "subject_cov": 0.5, "identity": 1.0, "score": 134.0, "evalue": 4.36e-39, "gene_start": 1, "gene_end": 73, "reference_start": 1, "reference_end": 73, "reference_sequence": "MSIKKQSFPNRLLNYKSIIVLIALLILMVISHLIVELIKDYHQYRMNVFIAYTCIFMVVFLYIIFSRQVYRRRWAVKLRKYNEYKLSSWEMKLVDRFYINAKYELRSIDYATIRLNILITIIILPSFIYALACMIAYIIKTTQSIT" }, "paralog": false, "identity": 0.9863013698630136, "score": 275.0, "evalue": 5.28e-97, "description": "Internal stop codon at 221863, 221908. Nonsense mutation around 221863, 221908.", "psc": { "uniref90_id": "UniRef90_A0AAC9X6J3", "product": "DUF3899 domain-containing protein", "uniref50_id": "UniRef50_A0AAC9X6J3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAC9X6J3", "UniRef:UniRef50_A0AAC9X6J3" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAC9X6J3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9X6J3" ], "product": "DUF3899 domain-containing protein" } }, "id": "NKIECMJPJD_407", "locus": "GCA000828735_00372" }, { "type": "cds", "sequence": "contig_1", "start": 221914, "stop": 222084, "strand": "+", "frame": 1, "gene": null, "product": "DUF3899 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI000165F8B4", "UniRef:UniRef100_A0AAC9X6J3", "UniRef:UniRef50_A0AAC9X6J3", "UniRef:UniRef90_A0AAC9X6J3" ], "nt": "ATGAAATTAGTTGATCGTTTTTATATCAATGCTAAATATGAATTACGATCTATTGATTATGCCACAATTCGCTTAAATATTTTAATTACAATTATCATTTTACCTAGTTTTATTTATGCATTAGCTTGTATGATTGCTTATATTATTAAAACCACACAATCAATTACTTAA", "aa": "MKLVDRFYINAKYELRSIDYATIRLNILITIIILPSFIYALACMIAYIIKTTQSIT", "aa_hexdigest": "11590faf78dfaea745cd27b9518327af", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000165F8B4", "uniref100_id": "UniRef100_A0AAC9X6J3", "db_xrefs": [ "SO:0001217", "UniParc:UPI000165F8B4", "UniRef:UniRef100_A0AAC9X6J3" ] }, "ips": { "uniref100_id": "UniRef100_A0AAC9X6J3", "uniref90_id": "UniRef90_A0AAC9X6J3" }, "psc": { "uniref90_id": "UniRef90_A0AAC9X6J3", "product": "DUF3899 domain-containing protein", "uniref50_id": "UniRef50_A0AAC9X6J3" }, "pscc": { "uniref50_id": "UniRef50_A0AAC9X6J3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9X6J3" ], "product": "DUF3899 domain-containing protein" }, "genes": [], "id": "NKIECMJPJD_408", "locus": "GCA000828735_00373" }, { "type": "cds", "sequence": "contig_1", "start": 222099, "stop": 222359, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATCAGAAAAACAGCTTTGAAGCTAATTCATCGCAAAACAATTATGGTGAATTGCCAATATTAAACCAAAAAAAACGAGTTAATTATTCATTTAGTAATAATTTTATTATTTTTGGTGGTGGTCGTTCACTAAAAACAGCAGCAGATCGCGATCGTGTTGTTGATCCATTAATCGATTTTTTCACTTTTGATTCAAAGTTTATGACATTTATTATTAAATCTTTGAAATTAATTGCTGAATTTTTCTTATTAGCTTGA", "aa": "MNQKNSFEANSSQNNYGELPILNQKKRVNYSFSNNFIIFGGGRSLKTAADRDRVVDPLIDFFTFDSKFMTFIIKSLKLIAEFFLLA", "aa_hexdigest": "cb758b85525b7c9e7427f9945a589f39", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9894.216599999992, "isoelectric_point": 9.453724479675294 }, "id": "NKIECMJPJD_409", "locus": "GCA000828735_00374" }, { "type": "cds", "sequence": "contig_1", "start": 222759, "stop": 222965, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTATTAATTGGTAAAGAAGTTGGATTACCATTTACGTTTGATATTAATAATTTTGTGACTTATATTTTACCAGGGTTAGCATTATCACTTGGTTCAATTATTGTTTATGTGCAATATATTAAAGTTGAAATGAATCGTGAATTAAACTCAATGCATGCGAAATTTGCGTATTTAAAAGGTGCAACTGTTAACCGTTTTGTGTGA", "aa": "MLLIGKEVGLPFTFDINNFVTYILPGLALSLGSIIVYVQYIKVEMNRELNSMHAKFAYLKGATVNRFV", "aa_hexdigest": "f23e19575b840ff7aa752d036578ea99", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7683.059699999999, "isoelectric_point": 9.157298851013184 }, "id": "NKIECMJPJD_410", "locus": "GCA000828735_00375" }, { "type": "cds", "sequence": "contig_1", "start": 223044, "stop": 223232, "strand": "+", "frame": 3, "gene": "oppB", "product": "Oligopeptide transport system permease protein oppB", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P6S4" ], "nt": "TTGTTTATTGAACAAATTTTTCAAATTCCAGGTTCAGGTTCATTATTTTTTGAAGCAATTATCTCAAAAGATTACAACGTGATTTTATTATTAGTAATTATTTATTCATTAATTACAATTATTGGTTATACAATTCGAGATGCTTTATATCAAATTATTGATCCACGTATAAGAAGAGGAGGAAAATAA", "aa": "MFIEQIFQIPGSGSLFFEAIISKDYNVILLLVIIYSLITIIGYTIRDALYQIIDPRIRRGGK", "aa_hexdigest": "6a7cf37ebab260f9358652ee6476caba", "start_type": "TTG", "rbs_motif": "4Base/6BMM", "psc": { "uniref90_id": "UniRef90_A0A3B0P6S4", "query_cov": 0.9838709677419355, "subject_cov": 0.8472222222222222, "identity": 0.639, "score": 81.3, "evalue": 4.87e-19, "valid": false, "product": "Oligopeptide transport system permease protein oppB", "uniref50_id": "UniRef50_A0A3B0P6S4" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0P6S4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P6S4" ], "product": "Oligopeptide transport system permease protein oppB" }, "genes": [ "oppB" ], "id": "NKIECMJPJD_411", "locus": "GCA000828735_00376" }, { "type": "cds", "sequence": "contig_1", "start": 223237, "stop": 223356, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCGAAGTTTGGAGAATATTTAAAACAAAAATCAAAAAGTATTACTTCATCATTTATAGATGAATTAACATCTGATGCACCAAATAAGTTTTTACAACCTTTTCAACACCAACAATGA", "aa": "MAKFGEYLKQKSKSITSSFIDELTSDAPNKFLQPFQHQQ", "aa_hexdigest": "f96e3f3a8cdda05c754d4385fa8850a8", "start_type": "ATG", "rbs_motif": "AGGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4519.051700000001, "isoelectric_point": 8.120776176452637 }, "id": "NKIECMJPJD_412", "locus": "GCA000828735_00377" }, { "type": "cds", "sequence": "contig_1", "start": 223399, "stop": 223620, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGAGGGCGTCCTCGTCCTTTTCGTGAATTTGCTAACCGATACGGACGTTCTTTTTCTGGATTAATCGGATTAGCAATTATTATTATTTTATTTATTTTGGCAATTATTATTCCTTTTACAACAGGATCACCAACAGAATTACGACCAAATAAACGATATTTAGATTATTTTACAGATGGTTTTATTTTTGGAACTGACAATAATGGGCGTGATTTATGA", "aa": "MGGRPRPFREFANRYGRSFSGLIGLAIIIILFILAIIIPFTTGSPTELRPNKRYLDYFTDGFIFGTDNNGRDL", "aa_hexdigest": "b645ae506f8d1f1572e866f6c1fdcb54", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8271.469199999994, "isoelectric_point": 9.968440055847168 }, "id": "NKIECMJPJD_413", "locus": "GCA000828735_00378" }, { "type": "cds", "sequence": "contig_1", "start": 223714, "stop": 223863, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGTTATTTTGAAATGTTTGATAAAATCATGACATTCATTATTAAAATTTTAACAAACGTTCCAACAATCATCATTTTAATGATTTTAACAATTATTTTAAAACCATCATTTGGAGTTATGATTTTTGCTATTTCATTTTCTGGTTGA", "aa": "MGYFEMFDKIMTFIIKILTNVPTIIILMILTIILKPSFGVMIFAISFSG", "aa_hexdigest": "693a728eb6ea02449f5d571e9019bd72", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5573.9254999999985, "isoelectric_point": 8.189692878723147 }, "id": "NKIECMJPJD_414", "locus": "GCA000828735_00379" }, { "type": "cds", "sequence": "contig_1", "start": 223993, "stop": 224235, "strand": "+", "frame": 1, "gene": null, "product": "ABC transmembrane type-1 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PSU9" ], "nt": "ATGATGATTACTCAATTAGTATTTAGTATTCCAGGAGCTATTTTAGCTGAATCATCACTTGCTTTTATTGGTTTATCTTTACCAAATACACCAACTTTAGGTAATTTAATTGCAGATGGTTCATCAATCATTACTTTATATCCACGTTTTACTTTAATTCCATCATTTTTACTAGTTTGCTTAGTTGCTTCAATTCAATTAGTTGGGGGTGCCACACAAGACGCATTAAGACGTCAAAGATAA", "aa": "MMITQLVFSIPGAILAESSLAFIGLSLPNTPTLGNLIADGSSIITLYPRFTLIPSFLLVCLVASIQLVGGATQDALRRQR", "aa_hexdigest": "1e280d2254de147a4616b8aafa112213", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A432FAT0", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.563, "score": 95.5, "evalue": 2.89e-24, "valid": false, "product": "ABC transmembrane type-1 domain-containing protein", "uniref50_id": "UniRef50_A0A3B0PSU9" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0PSU9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PSU9" ], "product": "ABC transmembrane type-1 domain-containing protein" }, "genes": [], "id": "NKIECMJPJD_415", "locus": "GCA000828735_00380" }, { "type": "cds", "sequence": "contig_1", "start": 224239, "stop": 224964, "strand": "+", "frame": 1, "gene": "dppD", "product": "Oligopeptide transport system permease protein", "db_xrefs": [ "COG:COG0444", "COG:EP", "SO:0001217", "UniRef:UniRef50_A0A084F1H7", "UniRef:UniRef90_Q9PPT0" ], "nt": "ATGAAACAAAAAAACAACCGATATACTTATATTAACTATCTTCGTAGTCAACGCGCTTTTATTCAAACATTAAAACCACAAATTTTAAAATTAACTTCAAATAATGTTTGTTTAAATGAACAAGAGGTGGATTTAGAACCACAATTAAATAATTTAGAAGACATTAATATACAATTAACTAATGAAAAAGTTAATATTCAAGATATTGTTACTTTAGAAGAAGAAATTAATGTTAAAGATAATAATCAAAATCAAAATTTAAATGAAGAAATCTTAGCAACAACATTAATTAATGAACAGGCTAGTGGTATTAATGAAATTAGTGAACAAAAAGAAACTACAATAGCTAATAAAAAACATAAAAAACAAACAATGGCTTTTAAAGCTGCTAATTCACGAAAAAACTTTGATTTTGAAAAATTTGAAAAAAATGTTAAATATAAAAGTTTTAAAGATGGTACAGTTAAAAAAATTGCAGCTGAAATCGAAAATGTACGTTTAACTTTTACAAACCCATCAAAACCCAATGATCGTGCTTTAGTTTTACGAAATACATCTGTTCAATTTTATGAAGGTGAAGTCCATGCAATTATTGGTGAATCAGGATCTGGTAAATCAGTAATTACTTCTCTTTTGTATGGTTTGGCTGGAAAAAATGCCAATGTTGAAGAAGGTAAAATTTTATTATATAACAATGAAGTTCAAGATTTTAGCTTCAAAGATTGA", "aa": "MKQKNNRYTYINYLRSQRAFIQTLKPQILKLTSNNVCLNEQEVDLEPQLNNLEDINIQLTNEKVNIQDIVTLEEEINVKDNNQNQNLNEEILATTLINEQASGINEISEQKETTIANKKHKKQTMAFKAANSRKNFDFEKFEKNVKYKSFKDGTVKKIAAEIENVRLTFTNPSKPNDRALVLRNTSVQFYEGEVHAIIGESGSGKSVITSLLYGLAGKNANVEEGKILLYNNEVQDFSFKD", "aa_hexdigest": "285d366d0cbdae629983f10e614796ee", "start_type": "ATG", "rbs_motif": null, "genes": [ "dppD" ], "truncated": "3-prime", "pseudogene": { "start": 224239, "stop": 225267, "observations": { "mutation": [ 224962 ], "stop": [ 224962 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PPT0", "query_cov": 1.012448132780083, "subject_cov": 0.4793713163064833, "identity": 0.98, "score": 352.0, "evalue": 8.37e-117, "gene_start": 1, "gene_end": 241, "reference_start": 1, "reference_end": 244, "reference_sequence": "MKQKNNRYTYINYLRSQRAFIQTLKPQILKLTSNNVCLNEQEVDLEPQLNNLEDINIQLTNEKVNIQDIVTLEEEINVKDNNQNQNLNEEILATTLINEQASGINEISEQKETTIANKKHKKHKKQTMAFKAANSRKNFDFEKFEKNVKYKSFKDGTVKKIAAEIENVRLTFTNPSKPNDRALVLRNTSVQFYEGEVHAIIGESGSGKSVITSLLYGLAGKNANVEEGKILLYNNEVQDFSFKDWEKSRYLGKVISAVFQNPMSTLNPTMKIGKQIMEGMLINGIVKTRKEAYNKSIEYLKLTKINNPEEIMELYPHELSGGMIQRVVIASIVSLHPKILVLDEPTTALDPTVQALVLDVIRELQEKFKMCIIFITHDLGVVASIANYISIMYAGQIIEEGQRDEILWYPQHPYTWGLIMSMPDVNKGDRLATIKGSVPSRLNDIVGDAFAIRNDYALTRDFEHEPEMYHVSETHRVKSALLDERAEKYTPPQIILDKWNNFKKRQENN" }, "paralog": false, "identity": 0.9826086956521739, "score": 556.0, "evalue": 1.66e-199, "description": "Internal stop codon at 224962. Nonsense mutation around 224962.", "psc": { "uniref90_id": "UniRef90_Q9PPT0", "gene": "DppD", "product": "Oligopeptide transport system permease protein", "uniref50_id": "UniRef50_A0A084F1H7", "cog_id": "COG0444", "cog_category": "EP", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PPT0", "UniRef:UniRef50_A0A084F1H7", "COG:COG0444", "COG:EP" ] }, "pscc": { "uniref50_id": "UniRef50_A0A084F1H7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A084F1H7" ], "product": "Oligopeptide ABC transporter, ATP-binding protein" } }, "id": "NKIECMJPJD_416", "locus": "GCA000828735_00381" }, { "type": "cds", "sequence": "contig_1", "start": 225016, "stop": 225453, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13304.12", "PFAM:PF00005.33" ], "nt": "ATGTCAACATTAAATCCAACCATGAAGATTGGTAAGCAAATTATGGAAGGGATGTTAATTAATGGAATTGTTAAGACAAGAAAAGAAGCCTATAATAAATCAATTGAATATTTAAAATTAACAAAAATTAATAATCCTGAAGAAATTATGGAATTATACCCACACGAATTATCTGGTGGAATGATTCAAAGAGTTGTAATTGCCTCAATTGTTTCATTACATCCAAAAATTTTAGTTTTAGACGAACCAACAACAGCGCTTGATCCAACAGTGCAAGCTTTAGTGTTAGATGTTATTCGTGAGTTACAAGAGAAATTTAAAATGTGTATTATTTTTATTACTCACGATCTTGGAGTGGTTGCTTCAATTGCTAATTATATTTCTATTATGTATGCTGGACAAATTATTGAAGAAGGTCAGCGTGATGAAATTTTATGA", "aa": "MSTLNPTMKIGKQIMEGMLINGIVKTRKEAYNKSIEYLKLTKINNPEEIMELYPHELSGGMIQRVVIASIVSLHPKILVLDEPTTALDPTVQALVLDVIRELQEKFKMCIIFITHDLGVVASIANYISIMYAGQIIEEGQRDEIL", "aa_hexdigest": "6c4249f25e5fe1abb950fd38a5048a9d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13304.12", "name": "AAA_21", "length": 73, "aa_cov": 0.4827586206896552, "hmm_cov": 0.9863013698630136, "evalue": 9.557200375071093e-09, "score": 31.23350715637207, "start": 51, "stop": 120 }, { "id": "PF00005.33", "name": "ABC_tran", "length": 83, "aa_cov": 0.5655172413793104, "hmm_cov": 0.8192771084337349, "evalue": 2.7426711228179398e-11, "score": 39.77769470214844, "start": 4, "stop": 85 } ], "seq_stats": { "molecular_weight": 16326.136099999982, "isoelectric_point": 5.375229454040528 }, "id": "NKIECMJPJD_417", "locus": "GCA000828735_00382" }, { "type": "cds", "sequence": "contig_1", "start": 225487, "stop": 225726, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAATGCCTGACGTTAATAAAGGTGATCGTTTAGCAACAATTAAAGGTTCTGTTCCATCACGTTTAAATGACATAGTTGGTGATGCATTTGCTATTCGTAATGATTATGCATTAACTCGTGATTTTGAACATGAACCTGAAATGTATCATGTAAGTGAAACTCATCGTGTTAAATCAGCATTATTAGATGAACGTGCTGAAAAGTATACACCTCCACAAATAATTTTAGATAAATGA", "aa": "MSMPDVNKGDRLATIKGSVPSRLNDIVGDAFAIRNDYALTRDFEHEPEMYHVSETHRVKSALLDERAEKYTPPQIILDK", "aa_hexdigest": "00319eb634895b44977cfd238923625a", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9014.072299999993, "isoelectric_point": 5.499706840515136 }, "id": "NKIECMJPJD_418", "locus": "GCA000828735_00383" }, { "type": "cds", "sequence": "contig_1", "start": 225780, "stop": 226523, "strand": "+", "frame": 3, "gene": "dppD", "product": "Oligopeptide transport system permease protein", "db_xrefs": [ "COG:COG0444", "COG:EP", "SO:0001217", "UniRef:UniRef50_A0A449AZS4", "UniRef:UniRef90_Q9PPT1" ], "nt": "ATGAGTGATACAAACCAGAAAAAAGCCTTTTTTAATCACAAAAAACGTTTTTGTTTACCAGACAAAATTACTATTCGCCCAGTTACTGAAGGTTTAATAGAACGATTAAATGCAAATCCTGAAAAAAAGCGTTTAGTACAAGTACGTCACTTAGATGTTACTTATGGTCATGGATTTAAAAAATTTAGAGCGGTTAAAGATATTTCATTTAATATTTATCAAGGTGAAGTTTTAGGATTAGTTGGTGAATCAGGATCTGGTAAATCAACAACCGGATCTGCGTTAATTGGTTTAGCGCGTCATAGTTTTGGGGATATTATTATTGATGGTATTAAATTGCCTAAACAAGGTGAGAAAGTTACTAAAGAACTAACTGATTTTATGGTTAATAATGTGCAGATGATTTTTCAAGATCCATCGAATTCACTTAATCCTTATGTAAATATTGAAACAGTTGTTTCAGAAGGATTAAATAATATTAAAGATTTGAAAAAATCTTTCTTACGTTTAATTTATAAAGATTATTTACGAGGATTAAAAAAAGAACTTAAAGCAAAAAATTTTTTAAATAATGAACAAGTACAAATTTTTTTAGAAAAAGCACAAGATTTAAAGTTTGATTATTTAAATGAAACTGCTCAACAAGAATTAATCCAATTATTCAATGAATTAGAATTAAATCATTTATCATTAGATAATAATGATGAAAAATTACAAAAAGCTTTTAATGATATTAATACTTGA", "aa": "MSDTNQKKAFFNHKKRFCLPDKITIRPVTEGLIERLNANPEKKRLVQVRHLDVTYGHGFKKFRAVKDISFNIYQGEVLGLVGESGSGKSTTGSALIGLARHSFGDIIIDGIKLPKQGEKVTKELTDFMVNNVQMIFQDPSNSLNPYVNIETVVSEGLNNIKDLKKSFLRLIYKDYLRGLKKELKAKNFLNNEQVQIFLEKAQDLKFDYLNETAQQELIQLFNELELNHLSLDNNDEKLQKAFNDINT", "aa_hexdigest": "832cc5ac9099df727e6c83c49dc7894e", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [ "dppD" ], "truncated": "3-prime", "pseudogene": { "start": 225780, "stop": 226826, "observations": { "mutation": [ 226521 ], "stop": [ 226521 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PPT1", "query_cov": 1.0, "subject_cov": 0.5626423690205011, "identity": 0.951, "score": 458.0, "evalue": 1.58e-159, "gene_start": 1, "gene_end": 247, "reference_start": 1, "reference_end": 247, "reference_sequence": "MSDTNQKKAFFNHKKRFCLPDKITIRPVTEGLLERLNANPEKKRLVEVRHLDVTYGHGFKKFRAVKDISFNIYQGEVLGLVGESGSGKSTTGSALIGLARHSFGDIIIDGVKLPKQGEKVTKELTDFMVNNVQMIFQDPSNSLNPYVNIETVVSEGLNNIKDLKKSFLRLIYKDYLRGLKKELKAKNLANNEQVQTFLEKAQDLKFDYLNETNQQELIQLFNELELANLSLDNNNKKLEKAFNDINTWKSKTRTDITRHLILDVLKSVGLDETVLRRYPLEFSGGQQQRIGISRAVALRPKLLIADEPISALDVSIQAQVVNIFKELKAKYDLTILFIAHDLRMVEYISDRIAVMNKGVLLEIGPTKEIVKNFLHPYTRSLMEAVPSIDSDKKSLLGYVYDANMHGYTHEKQPKWIHLGNDHYILGTEEEVADWKAGKY" }, "paralog": false, "identity": 0.9597701149425287, "score": 654.0, "evalue": 2.97e-239, "description": "Internal stop codon at 226521. Nonsense mutation around 226521.", "psc": { "uniref90_id": "UniRef90_Q9PPT1", "gene": "DppD", "product": "Oligopeptide transport system permease protein", "uniref50_id": "UniRef50_A0A449AZS4", "cog_id": "COG0444", "cog_category": "EP", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PPT1", "UniRef:UniRef50_A0A449AZS4", "COG:COG0444", "COG:EP" ] }, "pscc": { "uniref50_id": "UniRef50_A0A449AZS4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449AZS4" ], "product": "Phosphate ABC transporter ATP-binding protein" } }, "id": "NKIECMJPJD_419", "locus": "GCA000828735_00384" }, { "type": "cds", "sequence": "contig_1", "start": 226740, "stop": 227024, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAATATTTTTAAAGAATTAAAAACTAAATATGATTTAACAATTTTATTTATTGCTCATGATCTACGAATGGTTGAATATATTTCAGATCGAATTGCAGTTATGAATAAGGGTGTGTTGTTAGAAATTGGTCCAACAAAAGAAATTGTTAATAATTTCTTACATCCATACACACGTTCTCTAATGGAAGCTGTACCTTCAATTGATTCAGAAAAAAAATCACTATTAGGTTATGTATATGATGCTAACATGCATGAATATACACCAACAAAACAACCAAAATGA", "aa": "MNIFKELKTKYDLTILFIAHDLRMVEYISDRIAVMNKGVLLEIGPTKEIVNNFLHPYTRSLMEAVPSIDSEKKSLLGYVYDANMHEYTPTKQPK", "aa_hexdigest": "88c80cdfd5bf80237bf1d442be897424", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10920.632999999993, "isoelectric_point": 6.826954078674317 }, "id": "NKIECMJPJD_420", "locus": "GCA000828735_00385" }, { "type": "cds", "sequence": "contig_1", "start": 227118, "stop": 227567, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAAGGTTAAAATTATCAGCTAAAATTGCAATTGCTTTTGCATCAGTTGGTGCATTTGCTGGTGCTGTTGTTGGTTTTATGAAATTATATGCTGTTTCTACTGGTGGACTTGGACGTCAAACATTACAAGTTAACAGTAGTTTTGATACGACAATTCCTAATCGTCAAGTAGATCGTGCTTTATTAATGGATCAATTTGGCAAACCAGTTGCTGATTATGATTTTAAAGCAAAAAAAGGTAATAATACACAAGTAACATTATTAATTGATTTTGGAAAATATAAAAAAGGTCAGAAAATTTCTTATCTTGAATTTTTAGATAATTTTGTTAATTTAAATAACAACAACTTACCAAATTTAAGATTAGAAGTGGGCCCAATTGTTTTCAATAACAATTATATCAATAGTATTTCTCCAGACGAATTCATTGAGTTTACTAATTGA", "aa": "MKKRLKLSAKIAIAFASVGAFAGAVVGFMKLYAVSTGGLGRQTLQVNSSFDTTIPNRQVDRALLMDQFGKPVADYDFKAKKGNNTQVTLLIDFGKYKKGQKISYLEFLDNFVNLNNNNLPNLRLEVGPIVFNNNYINSISPDEFIEFTN", "aa_hexdigest": "0abc0760f969275a117cf9f24997af43", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16577.86159999999, "isoelectric_point": 9.583048057556148 }, "id": "NKIECMJPJD_421", "locus": "GCA000828735_00386" }, { "type": "cds", "sequence": "contig_1", "start": 227598, "stop": 229358, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTGACTTTGAAAGAATTTAAATTAAGTCGTGGAATTCAACAAAATGGTAACGCAATTACACTAGGGTTACATAGTGGTACAAGAGAAAGAACAGTAATTGAATTCTTCCCAGATGCTTTTTTTGGTTCATTACCAATTTATAATATTAATGCTGGCCCCGGCAATGCTTATGATTCATTAGCACGACAAGTTAATAAAGATGCTATGACATTAGAAGAATTAGAAGAATATCATCAAAAAATTCCTTTAATGATTTCAGCATATAATAGCCATAGTTTTGGTGGTGTTAAATTAATTGATGTCCTAAAAAACGCAAAAAATACTTATTTATTTAATGCTGATAAGTACTTTGATTTAACAAAAGATCCACTAAATTTTCCTAATTTAAAAAATCTTAAAGCAAGTGATCGAACATTAAATTATCTTTTTTATGCTCAAAACGAACAACAAGCAAAACAAAAAGTACAAGCATATTTAAACGAATTACAATTTGTTAAAGGGTTAATTCCAAAAGATAAAAAATTTAATCCTAAAATCGACGTTAAAATGGATGAAATTAATAAAATTAAAATTAATGAATTTTTGGAATTTAATGCTAGTGCTTCATCTCCTTTTGGTGGTTCGCTTGATCTTGAATCAATTGGTAAATCAAGTGGTAATCAAAATTATTTACTAATTGATGCAACCATTGGTGGCCAACAACAAAAAATTAAATACTACATGTTTAAGGATGAATTCACTACTAAATCAAGTTCAAATGATGAATTTATTCAAAATGATAATAGTATTTTAGCATCAAGTTTACGTGAAATAATTACTAAAAATTTTAATGATTATATTGATCAAAAACAAAATTTTAAAAATGTTTATGATGTTGAATCTACATTTAAAAATAAACAATTTTATATTTATGATATTGATGGTAATGGTAAAAATCCCCATTTTTATAAATCAAAAGAATCATTATTAGAATCTGAATTAGTTAACTATGATGAGAAAAAAGTTTCATTGGTAATTATTACTAAAACAAAAGTCGAAGGTTTAAATAAACTAATTTTAACAACAAAAGATGATAAAGATTTAATTTTAGAATCACCTAAAAATAATCCAAAAGGTTATAATTATGATTCAAGTTTTGATGATGCTTTCAACACTCTTAAAGTTGCGGCTAATTATTTTGATGTTTTTACACCTCGTTTGATTAAACAAGGAAGTAAATTTGTTAACGGCAAAATTATTAAAACTTATACTATTTATGTTGATGCATATAGCGGTTTATTAGATAAGGTTTTAAATAAAAATCGTCATTTATTACAAAAAATTAATGGTATTCATACAGAAGTTGTTCAAGATAAATATGGTAAAAATACATATAAAGTTGTTAATGGTGAATACGAAGGTATTTATGTAACTGATCGAATTCCTTATTTATCATTAGTTGCTCAATCTGATCCTGCATTTAAAACAACAGGTATTAACTATTTAAAATATGTAAGTACACACGAATATGGACATCACCAAACATTACAAGACATGAAAGATATTAGTGATAGTAATGATTCAGTTATTGGTGGAGGAATTGATTCTAGATCGGGAGTTAGTGATGAATCATATGTAAATGGTAAGGCATTACAAGATTATTTAAATGCACGTTCTAGCGGAATTACCTTTAGAAAAACTGATGTTGATTATAATCCAACAAAAGATGGGTCATTCTTTAATTTTTCATTAAATAACGATCCTAAAAATCCAGTTTGA", "aa": "MLTLKEFKLSRGIQQNGNAITLGLHSGTRERTVIEFFPDAFFGSLPIYNINAGPGNAYDSLARQVNKDAMTLEELEEYHQKIPLMISAYNSHSFGGVKLIDVLKNAKNTYLFNADKYFDLTKDPLNFPNLKNLKASDRTLNYLFYAQNEQQAKQKVQAYLNELQFVKGLIPKDKKFNPKIDVKMDEINKIKINEFLEFNASASSPFGGSLDLESIGKSSGNQNYLLIDATIGGQQQKIKYYMFKDEFTTKSSSNDEFIQNDNSILASSLREIITKNFNDYIDQKQNFKNVYDVESTFKNKQFYIYDIDGNGKNPHFYKSKESLLESELVNYDEKKVSLVIITKTKVEGLNKLILTTKDDKDLILESPKNNPKGYNYDSSFDDAFNTLKVAANYFDVFTPRLIKQGSKFVNGKIIKTYTIYVDAYSGLLDKVLNKNRHLLQKINGIHTEVVQDKYGKNTYKVVNGEYEGIYVTDRIPYLSLVAQSDPAFKTTGINYLKYVSTHEYGHHQTLQDMKDISDSNDSVIGGGIDSRSGVSDESYVNGKALQDYLNARSSGITFRKTDVDYNPTKDGSFFNFSLNNDPKNPV", "aa_hexdigest": "6b7d2053da291a4d99036e1d55bc0cbf", "start_type": "TTG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 66588.03060000007, "isoelectric_point": 7.124619865417481 }, "id": "NKIECMJPJD_422", "locus": "GCA000828735_00387" }, { "type": "cds", "sequence": "contig_1", "start": 229506, "stop": 229955, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATTCATTTGATCATGAATCTGCAACTGTTAACCCATCATTTGGCCCTGACATTAAAGATCCAAGTCGTTTAAAAGCTGAATATTATTTTTATAATGATCAAAATAGTCAAGAACAAAAAAATGATAATTTTAAATTTGGTAGTGTTATTGAAAAACCAGGACTTTCAAAATATGATGGTATTTTAAAAGATGGTATGGGAACACCAATTAAATTTAGTAAAGATGGTAGAGCAATTGTTTATAAACTTCACAAGAAAAAACATCCATACAAAAAAGATGATATTGAAATTTTAATTAAAACCAAAAACAATACACCAGTAATTGATTTATCAACTTGTTTAAAATCAGATGGGACAATTAACACAAGAAAACTTAATGAAAAAGTGAGAGAGATACAAGATTCAATTAATAGCCTAATCGTTAAAAATTACTATAATGGTGGTTGA", "aa": "MNSFDHESATVNPSFGPDIKDPSRLKAEYYFYNDQNSQEQKNDNFKFGSVIEKPGLSKYDGILKDGMGTPIKFSKDGRAIVYKLHKKKHPYKKDDIEILIKTKNNTPVIDLSTCLKSDGTINTRKLNEKVREIQDSINSLIVKNYYNGG", "aa_hexdigest": "780b1eaaad5975263f59b3373ae32d8b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16996.00359999999, "isoelectric_point": 9.127772331237793 }, "id": "NKIECMJPJD_423", "locus": "GCA000828735_00388" }, { "type": "cds", "sequence": "contig_1", "start": 230067, "stop": 230252, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTAAATACCCTGATTTTAACATTAGCAAATCTTTAAAATTAGATGAAAAAGCGAATGCATCATCTGTTCCATATTATAAAAAAGTTCTTTCGCAAATTATTGGCGAAGATATTACAAATAAAACCTTTGACCAATTTAAATATGCTTTATTAACTTTACAATACAATGCATCAAAAATTTGA", "aa": "MFKYPDFNISKSLKLDEKANASSVPYYKKVLSQIIGEDITNKTFDQFKYALLTLQYNASKI", "aa_hexdigest": "61acc190ace28e118362c9259a1a8520", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7071.0632000000005, "isoelectric_point": 9.223378944396973 }, "id": "NKIECMJPJD_424", "locus": "GCA000828735_00389" }, { "type": "cds", "sequence": "contig_1", "start": 230571, "stop": 230909, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATAAATTTAAATGAATTTGGAACTATTAAGTTGATTGTTGATAGAGGTGATGGAAAAGATAATAAACATTTAAAAAAGCTTGACGAATTAACAGAAAAAGAAATTTACCCATTAATGATTTTAACGCCTGCTGAAGCACAAGCAATTAATTTTGATTATCTAAAATCAAATAATGGATCATTCACTCCTCTTACACTTGAAAATAATGGATCAAAATTCTACACAATTAAATTTAAAGATATTCCTTCTTTAATTGAATTTATGTCTGTGGATCCAGCTAAATATACAATTGTGGAAAATGCTTTAAGCTCAAGTCATGAAAATGTTCGTAAATGA", "aa": "MINLNEFGTIKLIVDRGDGKDNKHLKKLDELTEKEIYPLMILTPAEAQAINFDYLKSNNGSFTPLTLENNGSKFYTIKFKDIPSLIEFMSVDPAKYTIVENALSSSHENVRK", "aa_hexdigest": "4a54122dd0ba3d2b44e4b1bb16b3970e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12725.408399999984, "isoelectric_point": 5.662380027770996 }, "id": "NKIECMJPJD_425", "locus": "GCA000828735_00390" }, { "type": "cds", "sequence": "contig_1", "start": 231120, "stop": 231245, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTTGATGGCAAACTAGGTTTATATGGATTTAGAAGTTTTGGTAATAAATATGAAAGACCAGGCTCACCATATGGAGAGCCACTTGATACATATAATTTTGCTGGTAATCCACAGAATGTTTGA", "aa": "MFDGKLGLYGFRSFGNKYERPGSPYGEPLDTYNFAGNPQNV", "aa_hexdigest": "0d71b8cdae9bd9ed575a06bda2ff5ac3", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4605.017800000003, "isoelectric_point": 5.970674705505371 }, "id": "NKIECMJPJD_426", "locus": "GCA000828735_00391" }, { "type": "cds", "sequence": "contig_1", "start": 232296, "stop": 232451, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATGGTTTCTTTAAAAAAGAATATAAGGATAAAGTTAAAAAAATTGCAATAAAAAATAAAAATAATGGCAAAATAGAATATATCGATGTTCACACAGATAATACAAATAATTTATTTTATCTAAAACGTCAATCAGACATTAATTCTAAATGA", "aa": "MYGFFKKEYKDKVKKIAIKNKNNGKIEYIDVHTDNTNNLFYLKRQSDINSK", "aa_hexdigest": "c6df0d89cd1df0bc17f2a1e3a8c004eb", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6143.978900000002, "isoelectric_point": 9.698639869689941 }, "id": "NKIECMJPJD_427", "locus": "GCA000828735_00392" }, { "type": "cds", "sequence": "contig_1", "start": 232575, "stop": 232769, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGATAAAGATCTAAAAGAAGTTATGGATTTAAGTGATCCAAAAAACCCAAAAAGTTTAATGACTTTAGGTTCACGCAAATATGTTGCTGAAAACGGTAAATCATATACTGTTTCACCAGTTTATGCAAGAAACGAAAATACAAAAGATAAAAATCGTACAATTATTAGAATTTCAAACCAATTCTCAGTTTAG", "aa": "MDKDLKEVMDLSDPKNPKSLMTLGSRKYVAENGKSYTVSPVYARNENTKDKNRTIIRISNQFSV", "aa_hexdigest": "b9bf11321dccc8017dad89ff733e4889", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7323.260600000004, "isoelectric_point": 9.571637153625485 }, "id": "NKIECMJPJD_428", "locus": "GCA000828735_00393" }, { "type": "cds", "sequence": "contig_1", "start": 232788, "stop": 233246, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAATAAATAAGAAAATTATATTTTTATCTATTGGAATTGTTGGTTTAATTCCCGTTGCTGCGATTATTGCTTCTTGTGCTAGAACTAAGACCACTAAAGAACAAAATAAAATTAGTCCATATTTAGAAGATGGTAGTAATTTTGGTTTGGCAATGGATGCAATCAACACATTGAATTATGTAAAATTTGCATCGCTTCGTAAAATTGTATTTTCACTAGTAGATGGTTTAATAAAAGAAGCGCCATCAACAAATTCAACAATCGGTAACTTATTAGGAATATCAAAGTTGTTTTTAAATCAATATAAAAATCCAGTTGATGCAGGTTTTAATAATATGACTGATGCACAATTAGAAGGTATGAGAATATCACCATCATATGTTGATTTATCATCTTTTGGAATTAATTCTGGAGCGTTAGCACCAACAGTTAGTGAAGATGATCCAACATGA", "aa": "MKKINKKIIFLSIGIVGLIPVAAIIASCARTKTTKEQNKISPYLEDGSNFGLAMDAINTLNYVKFASLRKIVFSLVDGLIKEAPSTNSTIGNLLGISKLFLNQYKNPVDAGFNNMTDAQLEGMRISPSYVDLSSFGINSGALAPTVSEDDPT", "aa_hexdigest": "2cc4f61222aa0e45d6927523f05bdf37", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16332.697999999978, "isoelectric_point": 8.663599967956543 }, "id": "NKIECMJPJD_429", "locus": "GCA000828735_00394" }, { "type": "cds", "sequence": "contig_1", "start": 233865, "stop": 234104, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGCAAGTGATGATTTACAAACTTTAGCTAATTCAAAAGATTTATTTAAATTTAAATCATTACCAACTTCACGTTTTGATTTACTAATGGAATTTGAGACTTATGCTCCAAAACCTTCATTTAGTGTTTTATTGACTGATATTTTAGGAAACCAAGCTTTACTACCAGTTAATCGTCGTTTTATTGAAACACATGGTGGAATTGAAAAATTCGGTTCTACTCTTGAAAATTTTGTTTGA", "aa": "MASDDLQTLANSKDLFKFKSLPTSRFDLLMEFETYAPKPSFSVLLTDILGNQALLPVNRRFIETHGGIEKFGSTLENFV", "aa_hexdigest": "e1652d3ca7009132296449942dc1399b", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8913.103699999996, "isoelectric_point": 5.180385398864746 }, "id": "NKIECMJPJD_430", "locus": "GCA000828735_00395" }, { "type": "cds", "sequence": "contig_1", "start": 234327, "stop": 234524, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCGTGATTTAATGAAAAAACAAGCAGGGGCTGGAACGGTTGCAATGCAATTTAATTTAGATCGTCAATCACGTTATGATAGTTATCAAGAATATTTAAAAAATCCTAATAAGGACATTCCAATCCTAGATCCTGATTTACGACGTGCAATTGCTTTTGCTATTAACAGAGAAAACATATTAAAATTATCTGGTTGA", "aa": "MRDLMKKQAGAGTVAMQFNLDRQSRYDSYQEYLKNPNKDIPILDPDLRRAIAFAINRENILKLSG", "aa_hexdigest": "8fb3fa5523bd3238f0d78d94e14168fd", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7513.5533, "isoelectric_point": 9.518063926696776 }, "id": "NKIECMJPJD_431", "locus": "GCA000828735_00396" }, { "type": "cds", "sequence": "contig_1", "start": 234972, "stop": 235136, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGACATTTGCAAATAGTGGTAAATTTGATATTTCTTATCAAAACTTTGATAAATATGCTGGTAGTACTTATGACTCAGCATTAGGATTATTTTTAACACCTGATGGAATTGATTTAAAAAATAATAAAAGCTCAGGTTATGAATTAAATCCAACAGGATCTTGA", "aa": "MTFANSGKFDISYQNFDKYAGSTYDSALGLFLTPDGIDLKNNKSSGYELNPTGS", "aa_hexdigest": "2047728d194933a83f1a971e225e7797", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5862.3135, "isoelectric_point": 4.460405921936036 }, "id": "NKIECMJPJD_432", "locus": "GCA000828735_00397" }, { "type": "cds", "sequence": "contig_1", "start": 235191, "stop": 235463, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAACGTTTACATATTAGTGAAGAAGACGCTGATATTATTCGTGAATTGGTAACGCCAACAAATATTAAAACAAGTGAAGTTTATAATCCACAACACCTAACAAATAGAGCTGATTTACGTAAATTAAAATTAAAAGATAAAGCCATTCCAATGTTTGATGTTAATTATTCTGAATATGACGCTAAGATCATTCATGATTTAGAACACCAAATTAATAAATTAAATCAAAAAATTAAAAATGAGCCTACAAGTAGTTCGAAATCAACGTGA", "aa": "MKRLHISEEDADIIRELVTPTNIKTSEVYNPQHLTNRADLRKLKLKDKAIPMFDVNYSEYDAKIIHDLEHQINKLNQKIKNEPTSSSKST", "aa_hexdigest": "052f6527268b7bf283afc7feb7a29544", "start_type": "TTG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10502.820699999991, "isoelectric_point": 7.987520027160644 }, "id": "NKIECMJPJD_433", "locus": "GCA000828735_00398" }, { "type": "cds", "sequence": "contig_1", "start": 235503, "stop": 235961, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAGAACGTGAATTTTATTTATCAGAAGAGTTCTTAAATAAACACGATCCAAAACGTGAAAATAAACCGTTTTTACTAGTATTGCAAAATAATGTAACAGGTCAATTTACAACGCTACAAATTCAACCAGTTATCGATAAAAAAACAAATCAATATGTTCAAGCTACAAAAGAAGATTATGCAAAATATTATAATAAAGAAAATATTGATGAAATTAATTTCAAAAAACGATTTAAATACAAATTTTCTATTAAAAAATTACCAATTGGATCATATCAAGTAATCAACTTATCTTACATAAATGATTTAAAACCATTATTAAGTAAGTACTTAAGAAATGAGAATAATGAAATATCTGATAAAGTTAATAATGAAATTTATATGACAAAATTAGAAACAGTTGTTGATCAACGTAAAAGAATTATTGATTTCTTTTCAGGGAATCGTCCAGGTTGA", "aa": "MQEREFYLSEEFLNKHDPKRENKPFLLVLQNNVTGQFTTLQIQPVIDKKTNQYVQATKEDYAKYYNKENIDEINFKKRFKYKFSIKKLPIGSYQVINLSYINDLKPLLSKYLRNENNEISDKVNNEIYMTKLETVVDQRKRIIDFFSGNRPG", "aa_hexdigest": "03c38d2faca79dde99ae34009e1c2d55", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18243.596599999993, "isoelectric_point": 9.31782512664795 }, "id": "NKIECMJPJD_434", "locus": "GCA000828735_00399" }, { "type": "cds", "sequence": "contig_1", "start": 236082, "stop": 236183, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAATAACTTTACATATTATTTACAATTTGCTTATGATTATCGAAGACCTCCACGTCCTGGTTTACCAACATCACCACAAGCAGAAGGGGATAATTAA", "aa": "MKNNFTYYLQFAYDYRRPPRPGLPTSPQAEGDN", "aa_hexdigest": "d5487bdb482ceddcf82e6d31143b4651", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3908.270800000002, "isoelectric_point": 8.138182640075684 }, "id": "NKIECMJPJD_435", "locus": "GCA000828735_00400" }, { "type": "cds", "sequence": "contig_1", "start": 236303, "stop": 236452, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCGTCATAATTTATTTGATGCATATACCCTAAATCATGAAAAAGAATTAATTAACATGTTAAATCCTAAAGATTTAGAAGTTTATTTATCACAAAAAGAACAAATTCGTTTATCAATTGGTTTTTTTGGCCTATTTTTAGTTTTATGA", "aa": "MRHNLFDAYTLNHEKELINMLNPKDLEVYLSQKEQIRLSIGFFGLFLVL", "aa_hexdigest": "2559ecf46ff3a56e94ebf54f89a1b403", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5852.780399999998, "isoelectric_point": 6.012735557556151 }, "id": "NKIECMJPJD_436", "locus": "GCA000828735_00401" }, { "type": "cds", "sequence": "contig_1", "start": 236386, "stop": 236574, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGGAATTAATGAAATAATTAAAGCTTTTTTAACTTTCGTTTTATTTTCATTAAAATCATTTAAATACTTAATTAAAATGCGAATATTAACAACTAAGCTAATTAAATATGTTAATGTTCATAAAACTAAAAATAGGCCAAAAAAACCAATTGATAAACGAATTTGTTCTTTTTGTGATAAATAA", "aa": "MKGINEIIKAFLTFVLFSLKSFKYLIKMRILTTKLIKYVNVHKTKNRPKKPIDKRICSFCDK", "aa_hexdigest": "0e633f300074a2fa21ee93e91146b88b", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7392.0479000000005, "isoelectric_point": 10.305674171447752 }, "id": "NKIECMJPJD_437", "locus": "GCA000828735_00402" }, { "type": "tmRNA", "sequence": "contig_1", "start": 237009, "stop": 237421, "strand": "+", "gene": "ssrA", "product": "transfer-messenger RNA, SsrA", "db_xrefs": [ "SO:0000584" ], "tag": { "start": 237092, "stop": 237178, "aa": "AENKKSSEVELNPAFMASATNANYAFAY", "nt": "GCAGAAAATAAAAAATCTTCTGAAGTAGAATTAAACCCAGCGTTTATGGCTTCAGCTACTAATGCAAACTACGCTTTTGCGTACTAA" }, "nt": "GGGGATGTCACGGTTTCGACGTGACACATTAATTTTTAATTGCAGTGGGGTTAGCCCCTTATCGCTTTCGAGGCATTTTAAATGCAGAAAATAAAAAATCTTCTGAAGTAGAATTAAACCCAGCGTTTATGGCTTCAGCTACTAATGCAAACTACGCTTTTGCGTACTAATTAGTTATTAGTAGAAACGTTCATTAACATAATTACTATTGGTTGGTTTTTGGGCTTATTTTACAATAGTTTTAAATTTAAAATTCTTATTTGTTGTTTAAATTTAAATAGATTTAACAAATAGTTAGTTAATTTTAAATTTGTTTTATTAGTTATTAACTACACTATTTTTAATAAAACTAAACTGTAGATATTATTAATTATGTGTTGCGGAAAGGGGTTCGACTCCCCTCATCTCCACCA", "id": "NKIECMJPJD_31", "locus": "GCA000828735_00403" }, { "type": "cds", "sequence": "contig_1", "start": 237526, "stop": 238035, "strand": "-", "frame": 3, "gene": "argA", "product": "N-acetyltransferase", "db_xrefs": [ "COG:COG1246", "COG:E", "RefSeq:WP_006688484.1", "SO:0001217", "UniParc:UPI00000C1CB9", "UniRef:UniRef100_A0AAC9T1W4", "UniRef:UniRef50_A0AAP9D7L1", "UniRef:UniRef90_A0AAC9T1W4" ], "nt": "ATGGTTTTGATAGAAAAAATCAATGAACAAGATATTAATACCGTCGTAGCATATGTTGTTGCTTTCCGTAAAAAACTTTATCCCAAAGTTGATCATAATAACTTACCTAAGGAACTAGTTAATTTTAAACAAACTTACATTATTAATCCTTTAGGAATATGGTTTAGTGTTTTTTTAGAAGATACACATGAATTAGTTGGAACAATTTCATGTCATGAATACGATTATAGATATAATAATCTATTCTATCTAGATAGAAGTACTAAAACAAGTGAGGTTGGTAAATTATATATTGATCCTTTGATTCGACGTCAAGGAATTGCAACTCGTTTGTTTAACGCATTAAAAGACCAAGCTAAAAAACAAGGAATTAAATGTTTTTATTTACACACACACCATCACTTGCCAGGCGCTAGAGAATTTTGGTTAAAAATGGGATTTAAAATTCAAAAAGAAATGACTTTAGAAGATGATGGTCAAGACATTATTCATATGACTTTAGAATTATAA", "aa": "MVLIEKINEQDINTVVAYVVAFRKKLYPKVDHNNLPKELVNFKQTYIINPLGIWFSVFLEDTHELVGTISCHEYDYRYNNLFYLDRSTKTSEVGKLYIDPLIRRQGIATRLFNALKDQAKKQGIKCFYLHTHHHLPGAREFWLKMGFKIQKEMTLEDDGQDIIHMTLEL", "aa_hexdigest": "46a633c9b28f01cf8ae83d16da6620c4", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00000C1CB9", "ncbi_nrp_id": "WP_006688484.1", "uniref100_id": "UniRef100_A0AAC9T1W4", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1CB9", "RefSeq:WP_006688484.1", "UniRef:UniRef100_A0AAC9T1W4" ] }, "ips": { "uniref100_id": "UniRef100_A0AAC9T1W4", "uniref90_id": "UniRef90_A0AAC9T1W4" }, "psc": { "uniref90_id": "UniRef90_A0AAC9T1W4", "gene": "ArgA", "product": "N-acetyltransferase", "uniref50_id": "UniRef50_A0AAP9D7L1", "cog_id": "COG1246", "cog_category": "E" }, "pscc": { "uniref50_id": "UniRef50_A0AAP9D7L1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAP9D7L1" ], "product": "GNAT family N-acetyltransferase" }, "genes": [ "argA" ], "id": "NKIECMJPJD_438", "locus": "GCA000828735_00404" }, { "type": "cds", "sequence": "contig_1", "start": 238204, "stop": 238686, "strand": "+", "frame": 1, "gene": "rpsF", "product": "30S ribosomal protein S6", "db_xrefs": [ "COG:COG0360", "COG:J", "GO:0000314", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022627", "GO:1990904", "KEGG:K02990", "RefSeq:WP_006688782.1", "SO:0001217", "UniParc:UPI00001350CF", "UniRef:UniRef100_Q9PPT6", "UniRef:UniRef50_Q9PPT6", "UniRef:UniRef90_Q9PPT6" ], "nt": "ATGGCGAAATACGAAATTATGTTAGTTGTTCGTGGTGATTTAGATCAAGAACAAGCTAATAAAGTTGCAAATGAATTAAAAGCAACTTTAAAGAATACAGAAGTAAAAGAAAATAATTATGATGGCGTGCAACAATTAGCTTATGAGATTAATAAGTTAAAAACAGCTTATCGTTATGTTTATAATTTTGAAACTACTGATGTTAGCCTAATTAATGAATTTAGACGTTTAGCAATTATTAATAAAAATGTATTGAGACATATTATTATTAATTTAGAAAAAGATTACGGTTATAAAGCAACTGTTAATACTAAAAAAGTACAACGTAATGAAAAACGTGCTGAAGTTTATGCACGTCAAAAAGAAGAAGCAGAACGTCGTGCAGTAGAACGTCAAGCAGCTTATGAAGCAATGAAAGCCGAACGAGAAGCAGCTGGTTTGCCAGTTAAAGAATTTGTTAAAAACACAAATTCAAAACGTTAA", "aa": "MAKYEIMLVVRGDLDQEQANKVANELKATLKNTEVKENNYDGVQQLAYEINKLKTAYRYVYNFETTDVSLINEFRRLAIINKNVLRHIIINLEKDYGYKATVNTKKVQRNEKRAEVYARQKEEAERRAVERQAAYEAMKAEREAAGLPVKEFVKNTNSKR", "aa_hexdigest": "09e7ad96fc14648e81116574cd17fb7d", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI00001350CF", "ncbi_nrp_id": "WP_006688782.1", "uniref100_id": "UniRef100_Q9PPT6", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001350CF", "RefSeq:WP_006688782.1", "UniRef:UniRef100_Q9PPT6" ] }, "ips": { "uniref100_id": "UniRef100_Q9PPT6", "uniref90_id": "UniRef90_Q9PPT6" }, "psc": { "uniref90_id": "UniRef90_Q9PPT6", "gene": "rpsF", "product": "30S ribosomal protein S6", "uniref50_id": "UniRef50_Q9PPT6", "cog_id": "COG0360", "cog_category": "J", "kegg_orthology_id": "K02990", "go_ids": [ "GO:0000314", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022627", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PPT6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PPT6" ], "product": "Small ribosomal subunit protein bS6" }, "genes": [ "rpsF" ], "id": "NKIECMJPJD_439", "locus": "GCA000828735_00405" }, { "type": "cds", "sequence": "contig_1", "start": 238731, "stop": 238877, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00436.31" ], "nt": "ATGAATAAAGTAATATTGATTGGTAATTTAGTACGTGATCCAGAAGCTCGTCAAATACCAAGTGGGCGTTTAGTTACTAACTTTACAATCGCTGTAAATGACAATACACCTAATGCTAATGCAAATTTTATACGTTGTGTTGCTTGA", "aa": "MNKVILIGNLVRDPEARQIPSGRLVTNFTIAVNDNTPNANANFIRCVA", "aa_hexdigest": "2c5eb9c90507e308c61ffe649885def2", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00436.31", "name": "SSB", "length": 54, "aa_cov": 1.0, "hmm_cov": 1.0, "evalue": 2.8493028187202056e-15, "score": 51.83924865722656, "start": 1, "stop": 48 } ], "seq_stats": { "molecular_weight": 5251.998200000002, "isoelectric_point": 9.48924655914307 }, "id": "NKIECMJPJD_440", "locus": "GCA000828735_00406" }, { "type": "cds", "sequence": "contig_1", "start": 239076, "stop": 239204, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATGGAATCATATACAGGTATTAATACTGATGCAGCATTTAGTTCAAACAAACCTCAAACTACCCTTTCATCGACAACATCAAATTTAAATAAAAATAATGATGAAGAAGATGAAATTACAAGTTGA", "aa": "MMESYTGINTDAAFSSNKPQTTLSSTTSNLNKNNDEEDEITS", "aa_hexdigest": "50d2c2f26a4940a45bc3649525f9e719", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4587.783, "isoelectric_point": 4.0500284194946286 }, "id": "NKIECMJPJD_441", "locus": "GCA000828735_00407" }, { "type": "cds", "sequence": "contig_1", "start": 239240, "stop": 239479, "strand": "+", "frame": 2, "gene": "rpsR", "product": "30S ribosomal protein S18", "db_xrefs": [ "COG:COG0238", "COG:J", "GO:0000314", "GO:0003735", "GO:0006412", "GO:0022627", "GO:0070181", "KEGG:K02963", "RefSeq:WP_006688517.1", "SO:0001217", "UniParc:UPI0000134DBA", "UniRef:UniRef100_Q9PPT8", "UniRef:UniRef50_Q9PPT8", "UniRef:UniRef90_Q9PPT8" ], "nt": "ATGGCCAAAGTAACTAATAACCGTAATCGTAAACCACGTAAAAAAGTTTGTATTTTATCTGCTAAAGGAATCGAACATGTCGATTACAAAGATGTAGAATTACTACAACGTTTTATTAACAATAATAACAAAATTGCATCACGACGTGTAACAGGAGCAAGTGCTCGTATGCAAAGAAGAATTGCAAATGCAATTAAACGTGCACGTTTCGTAGGATTACTTCCATACGTTAAAGAATAA", "aa": "MAKVTNNRNRKPRKKVCILSAKGIEHVDYKDVELLQRFINNNNKIASRRVTGASARMQRRIANAIKRARFVGLLPYVKE", "aa_hexdigest": "ba659bfab0c8569ca624e2dba836e1fc", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0000134DBA", "ncbi_nrp_id": "WP_006688517.1", "uniref100_id": "UniRef100_Q9PPT8", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000134DBA", "RefSeq:WP_006688517.1", "UniRef:UniRef100_Q9PPT8" ] }, "ips": { "uniref100_id": "UniRef100_Q9PPT8", "uniref90_id": "UniRef90_Q9PPT8" }, "psc": { "uniref90_id": "UniRef90_Q9PPT8", "gene": "rpsR", "product": "30S ribosomal protein S18", "uniref50_id": "UniRef50_Q9PPT8", "cog_id": "COG0238", "cog_category": "J", "kegg_orthology_id": "K02963", "go_ids": [ "GO:0000314", "GO:0003735", "GO:0006412", "GO:0022627", "GO:0070181" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PPT8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PPT8" ], "product": "Small ribosomal subunit protein bS18" }, "genes": [ "rpsR" ], "id": "NKIECMJPJD_442", "locus": "GCA000828735_00408" }, { "type": "cds", "sequence": "contig_1", "start": 239563, "stop": 240000, "strand": "+", "frame": 1, "gene": "rplI", "product": "50S ribosomal protein L9", "db_xrefs": [ "COG:COG0359", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904", "RefSeq:WP_045470364.1", "SO:0001217", "UniParc:UPI00045640CE", "UniRef:UniRef100_UPI00045640CE", "UniRef:UniRef50_Q9PPT9", "UniRef:UniRef90_Q9PPT9" ], "nt": "ATGAAAGTTATTTTATTAGAAGATATTGCAAGTCTTGGTAAAAAAAATGAAATTATTGATGTTAGTGATGGTTATGCAAAAAATTTTTTAATTCGACAAAAAAAAGCAGTTGCATTAACAAATAAATCACAAGAAGTTTTAAATAAAGATCTTGCAATTTTAGAAGCTCAAGAACAACAAGCAATCTTAGACGCAACTTTACTAAAAGATAAACTAGAGCAAAAGCCTCTACAATTTTTTTTAAAAACAAATAATTTACAAACTTTTGGATCAATTAGTAATAAACAAATTATTGATGAAATTAATAAAGAACAAAGATTTATAACAAAGCATATGATAACAAAACCACATGCATTAGGTATTGGTGAACATATTGTTGAAATTTTATTGCATAAAAAGGTTACTGCTAAACTTCATGTTATTGTTAGCAAAGAATAA", "aa": "MKVILLEDIASLGKKNEIIDVSDGYAKNFLIRQKKAVALTNKSQEVLNKDLAILEAQEQQAILDATLLKDKLEQKPLQFFLKTNNLQTFGSISNKQIIDEINKEQRFITKHMITKPHALGIGEHIVEILLHKKVTAKLHVIVSKE", "aa_hexdigest": "45a1f3848f3e3864252473683c38fbbe", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI00045640CE", "ncbi_nrp_id": "WP_045470364.1", "uniref100_id": "UniRef100_UPI00045640CE", "db_xrefs": [ "SO:0001217", "UniParc:UPI00045640CE", "RefSeq:WP_045470364.1", "UniRef:UniRef100_UPI00045640CE" ] }, "ips": { "uniref100_id": "UniRef100_UPI00045640CE", "uniref90_id": "UniRef90_Q9PPT9" }, "psc": { "uniref90_id": "UniRef90_Q9PPT9", "gene": "rplI", "product": "50S ribosomal protein L9", "uniref50_id": "UniRef50_Q9PPT9", "cog_id": "COG0359", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PPT9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PPT9" ], "product": "Large ribosomal subunit protein bL9" }, "genes": [ "rplI" ], "id": "NKIECMJPJD_443", "locus": "GCA000828735_00409" }, { "type": "cds", "sequence": "contig_1", "start": 240216, "stop": 240902, "strand": "+", "frame": 3, "gene": "dnaB", "product": "DNA 5'-3' helicase", "db_xrefs": [ "COG:COG0305", "COG:L", "EC:5.6.2.3", "KEGG:K02314", "SO:0001217", "UniRef:UniRef50_Q9PPU0", "UniRef:UniRef90_Q9PPU0" ], "nt": "GTGATTATTGATGAACAAGATCAAAATTATTTATTACATAAGAATTATTTACTTCAAATTTTAGATCGTACTGATTATTTAGTAGATTTAAAAGATTGTATTGAAATTATTAAGAATGCTTCAATTAAAAATAAACTTGATGTTTTTGCAAATGAGATTTTAGCAACCCAAATTTCATTAATTAACGCTAAAGATCAATTTAAAGAAATGCATGAAAAATTTTTAGAAATTCTTGCATCACGAACCGAAGATACTATTGAAAATATGGAATTAATTGCTAATCGATATTTTGAGAAATTAAATAAAATAGGCAATAGCGGGATTATTCCTGGAGTTATTAAAACTAAATACGATAATATTGATAAATTCACTAATGGTTATAAACCTGGAGAATTAGTTGTTATTGCCGCTCGTCCTGGAATTGGAAAAACTACATTTTGTTTAAATGTTATGGTTAACAATGTTAATGAAATTATTGAATATAACGAAAATATTCAACCAAATCAAAAAGAAAAGATCATTGTAATGTTTTCGCTTGAAATTACTAAAGAACAAATTTTACAAAAGTTTATCTCAATTAAAACAGGAATTTCTAATCGTGAAGTTATAGAAAATAAATATCGTATAACTAAAGGATATGACACAAGGTCTTTTGCTATGCAAGCAATCAATGATATTAAAAGTTGA", "aa": "MIIDEQDQNYLLHKNYLLQILDRTDYLVDLKDCIEIIKNASIKNKLDVFANEILATQISLINAKDQFKEMHEKFLEILASRTEDTIENMELIANRYFEKLNKIGNSGIIPGVIKTKYDNIDKFTNGYKPGELVVIAARPGIGKTTFCLNVMVNNVNEIIEYNENIQPNQKEKIIVMFSLEITKEQILQKFISIKTGISNREVIENKYRITKGYDTRSFAMQAINDIKS", "aa_hexdigest": "3f46696a3c3eaf2f6b0202cb8ceae0d6", "start_type": "GTG", "rbs_motif": null, "genes": [ "dnaB" ], "truncated": "both", "pseudogene": { "start": 240006, "stop": 241205, "observations": { "mutation": [ 240075, 240900 ], "stop": [ 240075, 240900 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PPU0", "query_cov": 1.0, "subject_cov": 0.4691358024691358, "identity": 0.956, "score": 429.0, "evalue": 6.15e-148, "gene_start": 1, "gene_end": 228, "reference_start": 71, "reference_end": 298, "reference_sequence": "MSTKDLNDAILDLFCLVINNNDFWKDVILRLEAKDFPEKVQQNIFNTIANLNEQKYKISESNILNGLGNYVIVDEQDQNYLLHKNYLVQILERTDYLVDLKDCIEIIKNASIKNKLDLFANEILSTQISLTNAKDQFKEMHEKFLEILASRTEDTIENMELIANRYFEKLNKIGNSGIIPGVIKTKYDNIDKFTNGYKPGELVVIAARPGIGKTTFCLNVMVNNVNEIIEYNQNIQPNQKEKIIVMFSLEITKEQILQKFISIKTGISNREVIENKYRIAKGYDTRSFAMQAINEIKSWPIFVDDRPNISIVDIEAKLYDLKKRYDIALVVLDYLQLVSAGNANKNMTRTQEVGRVSSALKVIAKEINAPVIAIAQLSRKAEERDVSSNANMKNNPLVKTIDNSPKLSDLRESGSIEQDADVVAFLHWDRKQRNAMQNDNQETRMRDDLIEAKFIVEKNRNGSTGETDIIFSKLNSKFIRATTSKE" }, "paralog": false, "identity": 0.9398496240601504, "score": 740.0, "evalue": 6.39e-273, "description": "Internal stop codon at 240075, 240900. Nonsense mutation around 240075, 240900.", "psc": { "uniref90_id": "UniRef90_Q9PPU0", "gene": "DnaB", "product": "DNA 5'-3' helicase", "ec_ids": [ "5.6.2.3" ], "uniref50_id": "UniRef50_Q9PPU0", "cog_id": "COG0305", "cog_category": "L", "kegg_orthology_id": "K02314", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PPU0", "EC:5.6.2.3", "UniRef:UniRef50_Q9PPU0", "COG:COG0305", "COG:L", "KEGG:K02314" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PPU0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PPU0" ], "product": "DNA 5'-3' helicase" } }, "id": "NKIECMJPJD_444", "locus": "GCA000828735_00410" }, { "type": "cds", "sequence": "contig_1", "start": 241014, "stop": 241466, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03796.21" ], "nt": "TTGGTTTCAGGAGGCAATGCAAATAAAAATATGACAAGAACACAAGAGGTAGGTCGTGTTTCAAGTGCATTAAAAATCATTGCTAAAGAAATTAATGCACCAGTAATCGCTATCGCTCAACTTTCACGTAAAGCAGAAGAACGTGATGTAAGCAATAATGTTAATATGAAAAATAACCCTTTAGTAAAAACAATTGATAATTCCCCAAAATTGTCTGATTTGCGAGAATCTGGATCAATTGAACAAGATGCTGACGTTGTTGCTTTTTTACATTGGGATCGAAAACAACGCAATTTTATGCAAAATGATAATCAAGAAACCCGAATGCGCGATGATTTGATTGAAGCAAAATTTATTGTTGAAAAGAACCGAAACGGGTCAACAGGTGAAACTGATATTATTTTTTCTAAATTAAATAGTAAATTTATTCGTGCAACCACTTCAAAGGAGTAA", "aa": "MVSGGNANKNMTRTQEVGRVSSALKIIAKEINAPVIAIAQLSRKAEERDVSNNVNMKNNPLVKTIDNSPKLSDLRESGSIEQDADVVAFLHWDRKQRNFMQNDNQETRMRDDLIEAKFIVEKNRNGSTGETDIIFSKLNSKFIRATTSKE", "aa_hexdigest": "f3b7b5a7006f7426c6c5783386b56edc", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03796.21", "name": "DnaB_C", "length": 137, "aa_cov": 0.9, "hmm_cov": 0.7956204379562044, "evalue": 1.3417780749206338e-32, "score": 108.67457580566406, "start": 8, "stop": 142 } ], "seq_stats": { "molecular_weight": 16893.849299999998, "isoelectric_point": 9.256193351745605 }, "id": "NKIECMJPJD_445", "locus": "GCA000828735_00411" }, { "type": "cds", "sequence": "contig_1", "start": 241469, "stop": 241819, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAAACAAAAGTAAAAAGCTAATTTTTTCAATATTAGGAATTAGTTTTATTGGATTAACGCCAATTGTTGCTGCATCGTGTTCAGCAGCTGCTCATCCTCAAGTTAATGTTCATTATGTTAGTGCTAATGCTACAACTAATAATATTGGTAAAATTAAAATTCGAGCCTTTGAAGGTAAGAATCAAAAACATGAAATGATTTTTGAGAATCAAAATTTATTAGATGATGTTTATTATGCGGCAGGTGATTTAACTTATACGAGTGTTAAAGATAATAAACGCTTTGATAAAAACAATAAAGAAATCGATCAATTTGGTTATTTGGTTCCCAAAAAAGAAGTTTGA", "aa": "MKKNKSKKLIFSILGISFIGLTPIVAASCSAAAHPQVNVHYVSANATTNNIGKIKIRAFEGKNQKHEMIFENQNLLDDVYYAAGDLTYTSVKDNKRFDKNNKEIDQFGYLVPKKEV", "aa_hexdigest": "d4cbb779ac64bf7060776ff86308997a", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13008.792799999988, "isoelectric_point": 9.433545875549317 }, "id": "NKIECMJPJD_446", "locus": "GCA000828735_00412" }, { "type": "cds", "sequence": "contig_1", "start": 241862, "stop": 242110, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAGTATTTTGCAAAATGAATTGATTCGTTTAATTGGTAATAAGTTAAATTTTGATTCAACAAGTAAATATGCTAATAATTTAGAAATTCTTGACCAAACAAAAAAACAAACTGTTTTTAACCAAAAACTATATTCAAGTGATGAATTAACAAAAATAAATAAGGTTAGTGAATTAATCAACTCTTTAGTTATTAGTGATGGATTGTATACTTATATTACCTCTATGACAGATCAATTAATAAAATGA", "aa": "MSILQNELIRLIGNKLNFDSTSKYANNLEILDQTKKQTVFNQKLYSSDELTKINKVSELINSLVISDGLYTYITSMTDQLIK", "aa_hexdigest": "6ce614afe25045b7e8ce1c6bec5fbf30", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9404.677699999998, "isoelectric_point": 6.139941215515137 }, "id": "NKIECMJPJD_447", "locus": "GCA000828735_00413" }, { "type": "cds", "sequence": "contig_1", "start": 242095, "stop": 242427, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTGAAGAGTTATATCAAGCATATTGTAATTTAATATTTTTTAAACGTACAAGAACATTTTTTAAACTTGGATTAACTAATAATTCATCATTTACTTTAACACCAAAATTAGGTTTATTAATACTTAAACCTGTAATTGCATCTACATATTCTCAATCAAAACTAAAATTATATAAAGCAAGTTTGTATGTATGTTTACCTGCACCTACACCTTGTACTAAAGCTAATAAAAATTCTTTAGCAACTTCGTTTTTTAACCCTTTAAAAAGTTCAAAATCTTTATTTGAATGGTTATTAAATGCATGTTCTGCTAATCATTTTATTAATTGA", "aa": "MFEELYQAYCNLIFFKRTRTFFKLGLTNNSSFTLTPKLGLLILKPVIASTYSQSKLKLYKASLYVCLPAPTPCTKANKNSLATSFFNPLKSSKSLFEWLLNACSANHFIN", "aa_hexdigest": "dc3ae9fe768a38317f680e55d7ba59ed", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12473.53549999998, "isoelectric_point": 9.733001518249512 }, "id": "NKIECMJPJD_448", "locus": "GCA000828735_00414" }, { "type": "cds", "sequence": "contig_1", "start": 242444, "stop": 242872, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAGCTAGTTCTAATGGTAGTCAACTAAATGCACTTAGTTCAATTAACGCTGCTTTATCAAGTGTTGATAAAAAAACTTATGCTGAAATTCATGATGATCACAAACCAGAAATTCGTAATTTAGTTTATGAAATTCCATTAAAAGATATGGTAGTTAATTTTGCACCATCAACAATGCGTTCTGCTAATATTAATCAAAAAGCAACACAAACTCTTCAAGAAAATTTTTTAAAAATTAAAAATCAAAAAACAGGGTTAACTGAGACAGAGAAAAAACGTACAACTGTTTATAATGAATACTACACAGGTACATTATCTAAAATTCATGCGTTTAGTATTTTGGGCCGCGATTTAAAACAAGAATTAAAAAACGAAGCTAAAGTTAATAAAAAAGAGTATGTTGAAGATAAAAAACTTGAAGACTGA", "aa": "MEASSNGSQLNALSSINAALSSVDKKTYAEIHDDHKPEIRNLVYEIPLKDMVVNFAPSTMRSANINQKATQTLQENFLKIKNQKTGLTETEKKRTTVYNEYYTGTLSKIHAFSILGRDLKQELKNEAKVNKKEYVEDKKLED", "aa_hexdigest": "8d115398ba4d34f55b200d6eeaa24375", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16168.075799999988, "isoelectric_point": 8.650577354431153 }, "id": "NKIECMJPJD_449", "locus": "GCA000828735_00415" }, { "type": "cds", "sequence": "contig_1", "start": 243086, "stop": 243217, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTGCTTTAGAAAATAAGGATGCTAATATTCATAATTTGACTAGTTTTAAAGCTGCTAAGAATAATTTTAGAAAATATCTTTTTAACCTTAATGAAATTAAAAGTGTAAATGTTAACTATAGCAAATGA", "aa": "MIALENKDANIHNLTSFKAAKNNFRKYLFNLNEIKSVNVNYSK", "aa_hexdigest": "6b8507937fe3024ff3529f407df478eb", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5016.689300000002, "isoelectric_point": 9.777226829528807 }, "id": "NKIECMJPJD_450", "locus": "GCA000828735_00416" }, { "type": "cds", "sequence": "contig_1", "start": 243433, "stop": 243681, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGATTTAGGAAATAATTTACCAGGATCACTAATCAATATTTTTAAAGCTTTAGAATATGATTTACAAATAAAAAGAACAAACCCTGATTTGTCTGATTGGGCAAAACAAGGAGTTTTATTGTTGAATACTGTTTTAACTGTTAACGCTCATCAACCAAATTCGCATAAAAATTTTGGTTATGAAGAATTAATTAAAAATGTGTTTAACGAACTTAGAAAACAAAAGCATGTTGTTTATTTATTATGA", "aa": "MDLGNNLPGSLINIFKALEYDLQIKRTNPDLSDWAKQGVLLLNTVLTVNAHQPNSHKNFGYEELIKNVFNELRKQKHVVYLL", "aa_hexdigest": "72bbd58da2aad1582ea9d64ab4766bf8", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9446.756899999998, "isoelectric_point": 8.044703483581543 }, "id": "NKIECMJPJD_451", "locus": "GCA000828735_00417" }, { "type": "cds", "sequence": "contig_1", "start": 243694, "stop": 243849, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTTACATTAATTTGATTGATCAAAAACAAAATTTAATTTTATGTGCTTCACATCCTTCGCCATTAAGTGCACATCGTGGTTTTTTAACTTGTAAACATTTTAGTAAATGCAATGATTATTTAATTAAACATTTACGCACTCCAATAAAATGA", "aa": "MSYINLIDQKQNLILCASHPSPLSAHRGFLTCKHFSKCNDYLIKHLRTPIK", "aa_hexdigest": "b1b830235f3ee58402e16c842af1e7b5", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5895.903299999999, "isoelectric_point": 9.508716011047362 }, "id": "NKIECMJPJD_452", "locus": "GCA000828735_00418" }, { "type": "cds", "sequence": "contig_1", "start": 243972, "stop": 244169, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTGTTTTTATTATCTTTACAGTTATTAAAAAAATCTATTATTCAATTCGTTTTCAAAAGCGTTTTTATATTATTCCTAAAACTTCGGTTAAAGGGATTTCAAATATTGCTATGGTGATTTCGATTTCTGTTGCAGTGATTATATTATTAACAGTTTTATCAGCTAACACAGCTTCTGTAATTTTTCGTGCTTGA", "aa": "MIVFIIFTVIKKIYYSIRFQKRFYIIPKTSVKGISNIAMVISISVAVIILLTVLSANTASVIFRA", "aa_hexdigest": "f3e60583b3fd2f6615fa308ea2dc542d", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7324.946399999999, "isoelectric_point": 10.76797695159912 }, "id": "NKIECMJPJD_453", "locus": "GCA000828735_00419" }, { "type": "cds", "sequence": "contig_1", "start": 244257, "stop": 244604, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACTGATTTATTATCTGTGGCTATGACTGCTGGTGTATTTCATTATGGTTATTTAATTGCTGCTATGGCTTATGGATTAATTGGAGGACTAATTCGAATTATTCTAACTACTTCCAAAAAAAAGGATTTACCATTTGCAATTTATAGTTCAATGGCAACTATTTTAATTGGTGTAGGAATTGCCTTATTTTTATATTTTGCTCCAGGAATTGGTGATAAGGGTTTTAATATTCAATTATTTGGTTTTGATATTAAAATTGCACGAACATTGATGATTGGCATTATTTTAGGTTTTTTCTTATTATCAATTATCGTTGTTTGGTTTAGTTTATTGATAAAATATTGA", "aa": "MTDLLSVAMTAGVFHYGYLIAAMAYGLIGGLIRIILTTSKKKDLPFAIYSSMATILIGVGIALFLYFAPGIGDKGFNIQLFGFDIKIARTLMIGIILGFFLLSIIVVWFSLLIKY", "aa_hexdigest": "6357ca13e61cb2890a9b3c2ba04aaa36", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12577.200399999983, "isoelectric_point": 9.522254371643069 }, "id": "NKIECMJPJD_454", "locus": "GCA000828735_00420" }, { "type": "cds", "sequence": "contig_1", "start": 244788, "stop": 244907, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTGGTTTTAAACTTATTGATTATTTATCCAATTTTTAAAATTGTTGTTCCTTTAATTCGTTATGATTATGAAGATGATATTGTTGAGGATAAACATATTCCAATTCATGTTGACTAA", "aa": "MVVLNLLIIYPIFKIVVPLIRYDYEDDIVEDKHIPIHVD", "aa_hexdigest": "ce7815c80289df7ad8446bfc50f87c58", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4664.504000000001, "isoelectric_point": 4.684465217590332 }, "id": "NKIECMJPJD_455", "locus": "GCA000828735_00421" }, { "type": "cds", "sequence": "contig_1", "start": 244928, "stop": 245212, "strand": "+", "frame": 2, "gene": null, "product": "Glu-tRNAGln amidotransferase C subunit", "db_xrefs": [ "RefSeq:WP_006688729.1", "SO:0001217", "UniParc:UPI00000C1CB4", "UniRef:UniRef100_A0AAC9X7P1", "UniRef:UniRef50_A0AAP9AD84", "UniRef:UniRef90_A0AAC9X7P1" ], "nt": "ATGAGTTATAATTTACAAAAATTAATGTCATCATGCATGTTTATATTAACTGATGATGAAATTAAACAGGTTGAAAATCGTTTAAATAATTTATTTAATGAAATTAAAGTTTTTGAGCTTTTTAATTTACAAAATATAACACCTTTTGAAATTATTAATAAATCCTTTGATAATTTTTTACGAGAAGATGAAATTAATTCTTGTGTAGGTAATAATAGCGAAGAAGTTCTAAATAATTGTCTAAAAGTAATCGATCATTATGGGGTATTAAAAAATGAAAAATAA", "aa": "MSYNLQKLMSSCMFILTDDEIKQVENRLNNLFNEIKVFELFNLQNITPFEIINKSFDNFLREDEINSCVGNNSEEVLNNCLKVIDHYGVLKNEK", "aa_hexdigest": "157da448b653e48039d75fec505f2a22", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI00000C1CB4", "ncbi_nrp_id": "WP_006688729.1", "uniref100_id": "UniRef100_A0AAC9X7P1", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1CB4", "RefSeq:WP_006688729.1", "UniRef:UniRef100_A0AAC9X7P1" ] }, "ips": { "uniref100_id": "UniRef100_A0AAC9X7P1", "uniref90_id": "UniRef90_A0AAC9X7P1" }, "psc": { "uniref90_id": "UniRef90_A0AAC9X7P1", "product": "Glu-tRNAGln amidotransferase C subunit", "uniref50_id": "UniRef50_A0AAP9AD84" }, "pscc": { "uniref50_id": "UniRef50_A0AAP9AD84", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAP9AD84" ], "product": "Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatC" }, "genes": [], "id": "NKIECMJPJD_456", "locus": "GCA000828735_00422" }, { "type": "cds", "sequence": "contig_1", "start": 245202, "stop": 246557, "strand": "+", "frame": 3, "gene": "gatA", "product": "Glutamyl-tRNA amidotransferase subunit A", "db_xrefs": [ "COG:COG0154", "COG:J", "SO:0001217", "UniRef:UniRef50_A0AAP9ADD2", "UniRef:UniRef90_Q9PPU5" ], "nt": "ATGAAAAATAAAACTATTTTAGCGCTTCATAATCAAATTAAAAAACGTGAAGTTAAAATTTATGATTTGATTAAAAAATCGATTGATCATGATTTAAAGATTAAAAATTTAAATGCAACTTTAGCAAATAATTATGAACAGGCATTGAAACAAGCAGCTTTTTTAGATGAAAAGATTAGTAGTGATAATTTAGAAATTGATTATTTATTTGGAATTCCATATTCTTTAAAAGACAACATTTCAACTAAGAATATTATTACAACAGGTGGTTCATTGTTTTTAAAAAATTACAATCCTCCATATGATGCGACAGTTAAAACATTATTAGATCAAAAACAAGCAATTTTGTTAAATAAATCAAATTTAGATGAATTTGGTTTAGGAGGCACTGGTACTGATTCAGCATTTGGCCCTGTAATTAATCCATTAAATCCAAAACATGTTAGCGGAGGTTCTTCATCTGGTAGTGCTGTTTTAGTTCAACAAGGCGTTGTACCTTTTGCAATTGCTACAGATACAGGAGATTCTGTTCGTCGCCCCGCTTCAATTATGGGTATTGTTGGTTTTAAACCGTCATATGGTCTTATTTCGCGTTATGGCGTTTACCCCTTTGCTCCTTCTCTTGATCATGTTGGAATTTTTACAAATAATATTTTTGATACGCAAATTGTTTTTAATGCTCTTGCTAAGCGAGATTTTAAAGATTTTACAAGTCTTAGTTTTAAAACACAATCACTTTCAATGCTAAAAACAAAAAATAAATATAAGATTGGTGTTTTAAAACCTAGTTTTGATTTATTAGCATCAAAATATCAACAAGAATTAACTAATCTGATTGTTAAATTAAATGATGAGCATGAAATAATTAATGTTGATTATGATATTAATTTATTAAAGGCAATTAGTCCAGTTTATAAATTAATCGCTTTTAGTGAGGCTTATAGTTCATACTCTAATTTTACAAACATCACTTTTGGCAAACATGATATTGATTATGATAACTATGAGGATTTAATTATTAAAACGCGAACTTTATATTTAGGAACACAATTAAAACAACGTTTTATGATAGGTGCTTATGTTACTCAAGAAAGCCAGTTTGAAACTTTATTAGTTGCTGCTAAAAAAATGCGTACATTAATAGTTGATCAAGCTAAAAATTTATTTAAAAATTGTGATTTAATTTTAAATTTAGCAGTTCATGATATAAGTGAAACAATTGATGATGTTAACAACCAAAAGCCCACAACTAATTTAGTTGAAGATGTAATGCAAATTGCTAATTTTGGTGGTTTTCCATCAATTGTAATCCCCTTTTTAACAACTAAATTAGGAAATTTAGGTTTAAATTTGTGA", "aa": "MKNKTILALHNQIKKREVKIYDLIKKSIDHDLKIKNLNATLANNYEQALKQAAFLDEKISSDNLEIDYLFGIPYSLKDNISTKNIITTGGSLFLKNYNPPYDATVKTLLDQKQAILLNKSNLDEFGLGGTGTDSAFGPVINPLNPKHVSGGSSSGSAVLVQQGVVPFAIATDTGDSVRRPASIMGIVGFKPSYGLISRYGVYPFAPSLDHVGIFTNNIFDTQIVFNALAKRDFKDFTSLSFKTQSLSMLKTKNKYKIGVLKPSFDLLASKYQQELTNLIVKLNDEHEIINVDYDINLLKAISPVYKLIAFSEAYSSYSNFTNITFGKHDIDYDNYEDLIIKTRTLYLGTQLKQRFMIGAYVTQESQFETLLVAAKKMRTLIVDQAKNLFKNCDLILNLAVHDISETIDDVNNQKPTTNLVEDVMQIANFGGFPSIVIPFLTTKLGNLGLNL", "aa_hexdigest": "a703320b57be2f2836b7b529a0b799f8", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_Q9PPU5", "query_cov": 1.0, "subject_cov": 0.9454926624737946, "identity": 0.998, "score": 875.0, "evalue": 0.0, "valid": true, "gene": "GatA", "product": "Glutamyl-tRNA amidotransferase subunit A", "uniref50_id": "UniRef50_A0AAP9ADD2", "cog_id": "COG0154", "cog_category": "J" }, "pscc": { "uniref50_id": "UniRef50_A0AAP9ADD2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAP9ADD2" ], "product": "Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatA" }, "genes": [ "gatA" ], "id": "NKIECMJPJD_457", "locus": "GCA000828735_00423" }, { "type": "cds", "sequence": "contig_1", "start": 246638, "stop": 247084, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02934.20" ], "nt": "ATGCAAAATTTTGAAGTTATTATTGGTATTGAAGTGCATACTGCACTTAATACAAAAACCAAAATGTTTTCTAATGCAGCAACATCGCATAAGAGTATTCCTAACACTTTAATTAATGAAATTGATTTAGCATTGCCAGGTACATTACCAACTGTTAATCAAGAAGTTGTTCATAAAGGACTTTTTTTAGCAAATGCATTACACATGCGTACTAATCATCAATTTATTGCTTTTGATCGTAAGCATTATTATTATTTAGACTTACCTAAAGGATATCAAATTACACAAAATTATTTTCCAATAGGTCAGAATGGTTATATTCAAATCATTGATGAATATAATAATTTAAAACGAATACGTATCAAACAAATTCATTTAGAAGAAGATACAGCCAAACAAACTAATATAGGTAATCAAATATATTTAGATTATAACCGTGCAGGATGA", "aa": "MQNFEVIIGIEVHTALNTKTKMFSNAATSHKSIPNTLINEIDLALPGTLPTVNQEVVHKGLFLANALHMRTNHQFIAFDRKHYYYLDLPKGYQITQNYFPIGQNGYIQIIDEYNNLKRIRIKQIHLEEDTAKQTNIGNQIYLDYNRAG", "aa_hexdigest": "dbc55417259b7264cfe0ab47a4c457d2", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02934.20", "name": "GatB_N", "length": 145, "aa_cov": 0.9662162162162162, "hmm_cov": 0.9862068965517241, "evalue": 2.2805438823148427e-51, "score": 170.1444854736328, "start": 6, "stop": 148 } ], "seq_stats": { "molecular_weight": 17090.25789999999, "isoelectric_point": 7.9748842239379885 }, "id": "NKIECMJPJD_458", "locus": "GCA000828735_00424" }, { "type": "cds", "sequence": "contig_1", "start": 247154, "stop": 247672, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02934.20" ], "nt": "TTGCGAAAAATTTTATTGTTTAATGATATATCAGATGCAAAAATGGAAGATGGATCATTACGTGTTGATGTAAACGTTTCAATTAGACCTCAAGGGGCTAAAAAATTTGGCACAAAAGTAGAAATTAAAAATATTAATTCTATTTCAAATGTTGCTAAAGCTATTGATTATGAGATAAGACGTCAATTAAATTTAATTTTACTAAATCAGAATGTTGAACAACAAACACGACGTTTTGATGATAATACGAATACAACGGTTTTTATGCGTTCAAAAAATGATGCTATTAATTATCGCTATATTCGTGAAGCAAATATTGCACCAATTCATCTAAGTGATGATTATGTAAAAAAATTGTTTTTAACTAAATCATGTAGTATTAATGATTTGCGTCAACAACTAGCACAAAAAGGTTTAGTTAGTTCTGCTATCGAACAGTTATTAAGTGATGGACCGTTGTTTAAAGCATTTAAATATGTAGACAAAATTGTTAATAATCCTTTAAGTGTTTATAAATGA", "aa": "MRKILLFNDISDAKMEDGSLRVDVNVSIRPQGAKKFGTKVEIKNINSISNVAKAIDYEIRRQLNLILLNQNVEQQTRRFDDNTNTTVFMRSKNDAINYRYIREANIAPIHLSDDYVKKLFLTKSCSINDLRQQLAQKGLVSSAIEQLLSDGPLFKAFKYVDKIVNNPLSVYK", "aa_hexdigest": "a24b4f780c3f4bc8d898a3bdbff827fd", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02934.20", "name": "GatB_N", "length": 116, "aa_cov": 0.6744186046511628, "hmm_cov": 0.9396551724137931, "evalue": 2.6606907534706345e-40, "score": 133.8424072265625, "start": 1, "stop": 116 } ], "seq_stats": { "molecular_weight": 19726.462799999987, "isoelectric_point": 9.590268516540526 }, "id": "NKIECMJPJD_459", "locus": "GCA000828735_00425" }, { "type": "cds", "sequence": "contig_1", "start": 247679, "stop": 247912, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02637.23" ], "nt": "TTGGAATTTATTGGTCTAATTAATAAAAATACGCAAAACATTGAAGAAATTACACCAGAATTATTGCAAAAAATTGCTAGAATGATCTTATTATTTGATCAAACTTTGATTAATGGAAAACAAACAAAAATAATTTTAGAAAAAATTTATTTGACAAATAAAGATCCTCAAATTCTAATTAAGGAATTAGGTTTTGAGCAAATTACTAATGAAAATGAAATTACTAAACTTTGA", "aa": "MEFIGLINKNTQNIEEITPELLQKIARMILLFDQTLINGKQTKIILEKIYLTNKDPQILIKELGFEQITNENEITKL", "aa_hexdigest": "6be684dd56d29286213497a78edf8c05", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02637.23", "name": "GatB_Yqey", "length": 78, "aa_cov": 0.987012987012987, "hmm_cov": 0.9615384615384616, "evalue": 2.292151984509119e-13, "score": 45.83795928955078, "start": 2, "stop": 77 } ], "seq_stats": { "molecular_weight": 8999.489699999995, "isoelectric_point": 5.05215663909912 }, "id": "NKIECMJPJD_460", "locus": "GCA000828735_00426" }, { "type": "cds", "sequence": "contig_1", "start": 247940, "stop": 248071, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02637.23" ], "nt": "ATGTTATTACAATACAATGAACGCCCCGACCGTGTTGAAAAATTTTTTATGGGTGAAATAATGAAATTAACTAAAGCACAAGCGAATCCAACCATTTCTTTTAATGTTTTAAAAAAAATCTTACAAAAATAA", "aa": "MLLQYNERPDRVEKFFMGEIMKLTKAQANPTISFNVLKKILQK", "aa_hexdigest": "ebd6cc6780bc1ef791e1502f5518549e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02637.23", "name": "GatB_Yqey", "length": 40, "aa_cov": 0.9302325581395349, "hmm_cov": 1.0, "evalue": 5.2819163418672775e-11, "score": 38.17214584350586, "start": 2, "stop": 41 } ], "seq_stats": { "molecular_weight": 5113.073299999999, "isoelectric_point": 9.871673011779784 }, "id": "NKIECMJPJD_461", "locus": "GCA000828735_00427" }, { "type": "cds", "sequence": "contig_1", "start": 248475, "stop": 248732, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAAGTGATGGAATAATAGCAGTAATGATATTTAATAATAATGTAAATGATGTTTTATCAACTACTGAAGCAAATATTTCGTTTTCAATATTAGCATCAACTGCTTTAAATGATGGTAAATTAGCTTCTTTTCCAAAAATTTTTCCTATTTTCTTTAATAATTTATCTAAATTAATTTCTGAAAAAGGTTCTCAAGTAATTTTATTTTTATCGAAACTAGCATTTAAAATCGAATTAGCTAAATCTCAACTGTAA", "aa": "MTSDGIIAVMIFNNNVNDVLSTTEANISFSILASTALNDGKLASFPKIFPIFFNNLSKLISEKGSQVILFLSKLAFKIELAKSQL", "aa_hexdigest": "1b881d439b7343f32eedc804b5aa7c3d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9254.716299999991, "isoelectric_point": 8.064882087707517 }, "id": "NKIECMJPJD_462", "locus": "GCA000828735_00428" }, { "type": "cds", "sequence": "contig_1", "start": 248798, "stop": 249520, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTAACAGAGCAGGGCGCCGCAGAATTCTTACCAGAATTTTTACGTTTAATTACTCAAATGTACACCTTCAAAACAACTCGTTATTTACAATATAAAGCCGGTTTAAAATTTGTTAATATTGAAGATGAAAAAGTACAACAACCATATACATACTTATTAGGTAAAATGCAAACAAAAACAAAAAGATTTGCTAATGAAATTAAAATATTGGGTATTAATTATGAAAAAAATAAATTTATTAATGGAAAACCAAGTCCTACTACTCAACTAAGTTTGATAAATGATCAGGGTGTTAATTTAATTGATCTAATTAAAAATGATGATCCTAATAATGAGATTCACAACATTATTATTAATAAAGTTGTCGCCCAAAAATATCATTTAAAGATTAATGATGTTTTTGAATATCAAATTAATAATCACATCAAACGTCTATCAAATAAATTTAATAACACACCAGAGAATTATAAAACTAAGTTTAAAGTTGTTGGGATTAATGATAGTTTAATGGATGAGCAATTAATTATTAATCAAAAAGTTGCAAATAAACTAATTGGTTTTGATGATTATCGCCGTCGGATTCAAATCGAAAATGGTTATCCAATCATTGAAGAACGCGAGTTCAATGGATTCTTTACAAAAGAAAAAGAACCTATTTTCTTTAATAATATGGCTTCATTTTATTCACCTACTGGACTATCACCAGCAATTGATTCATGA", "aa": "MLTEQGAAEFLPEFLRLITQMYTFKTTRYLQYKAGLKFVNIEDEKVQQPYTYLLGKMQTKTKRFANEIKILGINYEKNKFINGKPSPTTQLSLINDQGVNLIDLIKNDDPNNEIHNIIINKVVAQKYHLKINDVFEYQINNHIKRLSNKFNNTPENYKTKFKVVGINDSLMDEQLIINQKVANKLIGFDDYRRRIQIENGYPIIEEREFNGFFTKEKEPIFFNNMASFYSPTGLSPAIDS", "aa_hexdigest": "fb059554fb6f015b99b10ab7ef9b70b4", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 28080.82330000002, "isoelectric_point": 8.959767723083495 }, "id": "NKIECMJPJD_463", "locus": "GCA000828735_00429" }, { "type": "cds", "sequence": "contig_1", "start": 249797, "stop": 250642, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF07198.17" ], "nt": "TTGGATTATTCCAAAAATGGACGTTATTTATATGATTATTATGGAAGATTACCATATTTATACTTTAATCATTTACCACAAAATCTAATTAACCAAAAAGTTTATTTGACTTATACAATTAACCAAAATGATGAATTTCGAATTTATGGTATTGTTAATAAAGAAAAAGAAGTAGTTTTTGACTTAACAAAATTAAAAATTAATACCCAACAAAGTTATCAATTACGCGATATGCGAATCGCAAATAGTGATTTATTAATTTATGATAATAAACAACATGAATTTAATAATAATATTTTATATTTTGATAACAATTATTACGGTTTAAACAATAATAATCAAAAGCAAATTAATATTGATTACTTAACTCCTAATCGTAATCTTTATTTAACTTTAAAAGCAATGGGTAATGAATTTGTTAATAAGAAAAAAATACCTTTTATAATGACAAAAGCAATGGCTTCTTTAGTTGAACCTGATACTGCAATTTTAGATTATAATGAATTAGAAAAGGGTTATGTTTATCAAATTAGTACAGCAACACTAGAGGGTAGTGATACAAACTTATACTTAGATAACGTTAATTCTATTGATAAAACAAAAGATAATGGCTTACCTTATTCGTCTCTAAAATACAATAACGCAATGCGTGAAATTAAAAATTTAGGTACTGATCATCCAGTCTATGGCAAAATGTTATCAAATGTTTATTATCCATCAATTAGTACCCAACCTGAAATCTTACAAAATATTCGTTTTTTTGATAGTAAAGTAATTTCAAAAAGTTCTTTAGATGGAAATATTAGTGTTAAAGCAACGGATGTTAACATTGAATTTAACCCTTGA", "aa": "MDYSKNGRYLYDYYGRLPYLYFNHLPQNLINQKVYLTYTINQNDEFRIYGIVNKEKEVVFDLTKLKINTQQSYQLRDMRIANSDLLIYDNKQHEFNNNILYFDNNYYGLNNNNQKQINIDYLTPNRNLYLTLKAMGNEFVNKKKIPFIMTKAMASLVEPDTAILDYNELEKGYVYQISTATLEGSDTNLYLDNVNSIDKTKDNGLPYSSLKYNNAMREIKNLGTDHPVYGKMLSNVYYPSISTQPEILQNIRFFDSKVISKSSLDGNISVKATDVNIEFNP", "aa_hexdigest": "9b525332d887912be1b10ec0283129da", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07198.17", "name": "DUF1410", "length": 59, "aa_cov": 0.2099644128113879, "hmm_cov": 0.9661016949152542, "evalue": 1.543231619894022e-12, "score": 43.27106475830078, "start": 26, "stop": 84 } ], "seq_stats": { "molecular_weight": 32850.65050000005, "isoelectric_point": 6.328703498840333 }, "id": "NKIECMJPJD_464", "locus": "GCA000828735_00430" }, { "type": "cds", "sequence": "contig_1", "start": 250679, "stop": 251542, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF07198.17" ], "nt": "GTGTTAATTTATTATATTTTAAAACATTCAACCGCAGAGACAATGAAAGGCGATGCAGGTTTTAAAATCAATAAATTTATCATTAAAACAAAAGCAATCTTTTTAAAATTTGGTGCAATTCGAGTTTTAAAAGCTACTTATGTTGTTGCCAATTTAGCAAGAATGTTGATTTTATTAGTAATCGCTTCCTCTTTTTCTATTATTGCTTCTATTATTGCAACAACAAAAAATAATTTTAGTCAGTCAGCAAGTATTACTAATGCTAAACGTGAATATCAATATGCAGTTGATTTATATTCACCAACAGAACAGGGAGGATTTTATTACATTTCAAAGTTTGATGAAATGGGTGTTTCTAATGAATATGCTCCTTTAAAACAAATTGTTAAAAATGTTAAGATTGTAATTAAAATTAAAAATTTAGTTAATAAAAAAGTCAATGTTGTATATATCAATAATCAAACAAAGCAAAAAGTAATTATTGAAGCACTTATTAATGATAAGGGAATAATCACTTTTAAAACTAATCAGCTTGTTAAAGGTAGTTTTCAATTTGAAGGTATTTATGAACAAGAAACAAATAGAGTTTTAATTAATTCACAAAATTTAAATCCATCATTATTAAATATAGATAATCGAAAGATAGGCCATTTTATTTACTCTAATCAAAAACAATATATCTATTTACTAAAATCATACTTTAATGACAATGATGTAATTTTAGATTATGTTGATGCTAATAATCAACATACTTATTTAAAAGCAAAAACTCTAGGTCAATACTTGGTATTTGATCCTTCACAATTAAAACAAAAACAAAAATATCAATTAGTTAATGTTATAAACCATAAAGGAAATACATGA", "aa": "MLIYYILKHSTAETMKGDAGFKINKFIIKTKAIFLKFGAIRVLKATYVVANLARMLILLVIASSFSIIASIIATTKNNFSQSASITNAKREYQYAVDLYSPTEQGGFYYISKFDEMGVSNEYAPLKQIVKNVKIVIKIKNLVNKKVNVVYINNQTKQKVIIEALINDKGIITFKTNQLVKGSFQFEGIYEQETNRVLINSQNLNPSLLNIDNRKIGHFIYSNQKQYIYLLKSYFNDNDVILDYVDANNQHTYLKAKTLGQYLVFDPSQLKQKQKYQLVNVINHKGNT", "aa_hexdigest": "6681d4d1855ceb8d6a92e6b89934a892", "start_type": "GTG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07198.17", "name": "DUF1410", "length": 57, "aa_cov": 0.1951219512195122, "hmm_cov": 1.0, "evalue": 3.316852428654714e-10, "score": 35.800994873046875, "start": 231, "stop": 286 } ], "seq_stats": { "molecular_weight": 32871.78610000003, "isoelectric_point": 9.698124122619632 }, "id": "NKIECMJPJD_465", "locus": "GCA000828735_00431" }, { "type": "cds", "sequence": "contig_1", "start": 251753, "stop": 252088, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCCAATTTTAGAGTATTACACACTAAAAGTTAATACTAATGATCTTAATTTATTACAAAAAAATAATGTTTATAATGAAATAAAATCAGAATTATTAGATTCTAAAAAAGGTAACATATATAAGAGAACTGATAAGGATAACCCTTATCCAATTTATGTTAAACGAACCTTTTTTTTAACACAATTGAGGAATTTAATTATTGCAATTGCACTTAGTTTGATTGTTTTAATCTTATTGTTAGGAATCTACTTTGTTACCTCAAGTATTAAAAATATGATTAATAAAAACAAAGTCATGTTCGGTGCTATGCAAGCTCAAGGCGTATCAAGATGA", "aa": "MPILEYYTLKVNTNDLNLLQKNNVYNEIKSELLDSKKGNIYKRTDKDNPYPIYVKRTFFLTQLRNLIIAIALSLIVLILLLGIYFVTSSIKNMINKNKVMFGAMQAQGVSR", "aa_hexdigest": "54f1f352651a3ef0b962abbbba68d02d", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12833.063999999988, "isoelectric_point": 9.801338005065915 }, "id": "NKIECMJPJD_466", "locus": "GCA000828735_00432" }, { "type": "cds", "sequence": "contig_1", "start": 252284, "stop": 252532, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTATTGCCGTTTACAATTAATATTATTAATCGTAGTTCAGATCTAGCATACGTAAGCACATCTTATATTAAAGCTAATCAAAGAACAACAAAAAATATAAATAATTATAAAGAAATTGGTAATTATGAAGATTTTTTACAAGCACTAAAACTTCCATATCGTATAATCGATGATCAACCAAACTATCGTGAAATTACTTTGCCAAACGGTCGTAAAAAAAGAATTGCTAAAGATTATTATAGTTGA", "aa": "MLLPFTINIINRSSDLAYVSTSYIKANQRTTKNINNYKEIGNYEDFLQALKLPYRIIDDQPNYREITLPNGRKKRIAKDYYS", "aa_hexdigest": "5d304510b3cd2f786df27bc82b327412", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9707.94599999999, "isoelectric_point": 9.557905387878417 }, "id": "NKIECMJPJD_467", "locus": "GCA000828735_00433" }, { "type": "cds", "sequence": "contig_1", "start": 252668, "stop": 252949, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGTTGCTATTTATAAAAATGCAGATAGTAATAGCGACCAAAAAGAATATTATTTTAACGGCTATGTTAAAAATCAACGAGCTTATATTAATGTTAAATATAATAAAAATCTGCCAACACGTTTAAAATTAATTAATATTATTAAACAAAATCAAATAAATCAAAAAAACAATTTAGTTAATGTTTTAGATCAAGATTTAAATGATTATGCAAAGATTATTAATTTTAAAAAAGGTATTAAAGATATTAATACAAACCAGCAATCAGTTTATGATCAGTGA", "aa": "MVAIYKNADSNSDQKEYYFNGYVKNQRAYINVKYNKNLPTRLKLINIIKQNQINQKNNLVNVLDQDLNDYAKIINFKKGIKDINTNQQSVYDQ", "aa_hexdigest": "ab041729b83b96d8f53d3b5ff45f62b3", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10991.292999999989, "isoelectric_point": 9.522447776794433 }, "id": "NKIECMJPJD_468", "locus": "GCA000828735_00434" }, { "type": "cds", "sequence": "contig_1", "start": 253151, "stop": 253366, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTGCAAATTAATGAAAAAAATAAAAATAGTCAAAATCCAAATAAAAAATTTAAGCGAATTTTTATTAATGATGATAATTCAAATAAAATTAATAACTTTTATGACCTTTATTCAAATTACATTAAAGTCATTCAAATTTTAAAAAATAGTTATGAAAAAGATCCAGATACTGATAAAGATTTACTAGTTGAAGAGCGTGATTATACTCCTTGA", "aa": "MVQINEKNKNSQNPNKKFKRIFINDDNSNKINNFYDLYSNYIKVIQILKNSYEKDPDTDKDLLVEERDYTP", "aa_hexdigest": "f9025ea7460b2dd651f8f36f57c1c660", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8575.456299999998, "isoelectric_point": 6.181035804748535 }, "id": "NKIECMJPJD_469", "locus": "GCA000828735_00435" }, { "type": "cds", "sequence": "contig_1", "start": 253376, "stop": 254395, "strand": "-", "frame": 2, "gene": null, "product": "Unique hypothetical", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PPU8", "UniRef:UniRef90_Q9PPU8" ], "nt": "TTGGTTTATGAATATCATAATTCTAAAAATCAATTAGTAGGATTTCAAAATTTAGATGGTGGGCATATTTTATTTAAAACACCGGCGTTAAAATCAGCAAACGGTAATTATGAAACAACATTTAAAATTAATGCTGCAATTGTCCAAAAAAATAAACCATTTAAATCTTTTTTTAACTTTGATAAATATAAAGAATTAGGATTTAAGAATAAATCTTATTTTACAAATCCTGTATTAATTAAAAATATAGATCTTTATTCAAAACAAGTTGTTCAAACACTAAACGATCGTTCAAATCAATCAATTGATTTTAAAGACTATCAAAGCACATTTCAAGCCATTGTTGATACACTTGAAACTAACACGATAAATAAAGATTTTTATAGTGTGAATTTACATGTTGATTTTAACACACTATCAACAAAATACCTACTATTTGCTAATTATTTAAAAAATGAAGGTATTGATAAAAACCGTTATATAAATGAATTAAAAATCGATAACTATAAACAGAAGTATTTAGATTTATTTCAAAACCAAGTTTTAATTCCAAAAGAATTAATTGACGATCCAAAAAAAATAGAAGAAGTGCAAAAAATTATTGCAAAAGCCAAAGCCTTGTTAATTGCTAAAATTCGTGAAGAAACAAATATTTTTTATGAAAACTCACAAAAATCAGCTCTTGAAAATAAAAATAAGATTCGTGATTATGAGATGCAATTAAAACAAATAAATATTGCTCTACAAAATTCGAATTATTCTAATAACGAACTTATTCAATTAAAAAATGAACAAAAAGTATTAGCAGCAAATCTCGATCAAGCAAAATTTAAACACTTTTTATATAATGATTTAATTAAACAAGATTTCCGTTCGATTTTAAATAATTATAATATTTATTATCGCAAACATGAATCAGCAATTTATAATGATGTTAAAAGTGATTCAAATTTTATTGTCACAAATGCAAGCAATCCAACATATACACAAAACGGTTTAATTGCACCAAGAAATCAATGA", "aa": "MVYEYHNSKNQLVGFQNLDGGHILFKTPALKSANGNYETTFKINAAIVQKNKPFKSFFNFDKYKELGFKNKSYFTNPVLIKNIDLYSKQVVQTLNDRSNQSIDFKDYQSTFQAIVDTLETNTINKDFYSVNLHVDFNTLSTKYLLFANYLKNEGIDKNRYINELKIDNYKQKYLDLFQNQVLIPKELIDDPKKIEEVQKIIAKAKALLIAKIREETNIFYENSQKSALENKNKIRDYEMQLKQINIALQNSNYSNNELIQLKNEQKVLAANLDQAKFKHFLYNDLIKQDFRSILNNYNIYYRKHESAIYNDVKSDSNFIVTNASNPTYTQNGLIAPRNQ", "aa_hexdigest": "1196aa99424c222e2c0ec8b59bc41020", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 253079, "stop": 254695, "observations": { "mutation": [ 253153, 253378, 254449, 254488 ], "stop": [ 253153, 253378, 254449, 254488 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PPU8", "query_cov": 1.0, "subject_cov": 0.41955445544554454, "identity": 0.997, "score": 650.0, "evalue": 3.89e-228, "gene_start": 1, "gene_end": 339, "reference_start": 173, "reference_end": 511, "reference_sequence": "MKKMFLLMKNMMRLFFKNKTLVTQLSILMIVSTVVIVATTITVQRLQKSQSDIKKIGLQSDFLIDTKEQDNINFNEIDVSNNLVKHNEKLIQLATFNQNILKNSNNYLQLVDNAFHNHNLFWPTNVNNQKIDVLNLEGNIDWNKINQLKLQTVFWFENNKENNQNFANNYDDLVYEYHNSKNQLVGFQNLDGGHILFKTPALKSANGNYETTFKINAAIVQKNKPFKSFFNFDKYKELGFKNKSYFTNPVLIKNIDLYSKQVVQTLNDRSNQSIDFKDYQSTFQAIVDTLETNTINKDFYSVNLHVDFNTLSTKYLLFANYLKNEGIDKNRYINELKIDNYKQKYLDLFQNQVLIPKELIDDPKKIEEVQKIIAKAKALLIAKIREETNIFYENSQKSALENKNKIRDYEMQLKQINIALQNSNYSNNELIQLKNEQKVLAANLDQAKFKHFLYNDLIKQDFRSILNNYNIYYRKHESAIYNDVKSDSNFIVTNASNPTYTQNGLIAPRNQWIDNMVQINEKNKNSQNPNKKFKRIFINDDNSNKINNFYDLYSNYIKVIQILKNSYEKDPDTDKDLLVEERDYTPWINTSLINIYQLPQFFNILFNQSEWLLSIQYLKEMSDYKKYLLQIFEPYQDKEWIFNLKHKDWQDGEQVVAIYKNADSNSDQKEYYFNGYVKNQRAYINVKYNKNLPTRLKLINIIKQNQINQKNNLVNVLDQDLNDYAKIINFKKGIKDINTNQQSVYDQWALNNPQDYAMFKIWLSKIIKRYYFLRFSNHLMDIQFKHKTFYQKLCYCRLQLILLIVVQI" }, "paralog": false, "identity": 0.9925650557620818, "score": 1068.0, "evalue": 0.0, "description": "Internal stop codon at 253153, 253378, 254449, 254488. Nonsense mutation around 253153, 253378, 254449, 254488.", "psc": { "uniref90_id": "UniRef90_Q9PPU8", "product": "Unique hypothetical", "uniref50_id": "UniRef50_Q9PPU8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PPU8", "UniRef:UniRef50_Q9PPU8" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PPU8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PPU8" ], "product": "Unique hypothetical" } }, "id": "NKIECMJPJD_470", "locus": "GCA000828735_00436" }, { "type": "cds", "sequence": "contig_1", "start": 254486, "stop": 254911, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002D2A8F1", "UniRef:UniRef50_UPI0029075D7D", "UniRef:UniRef90_UPI0029075D7D" ], "nt": "ATGAAAAAAATGTTTCTATTAATGAAGAATATGATGCGTTTATTCTTTAAAAATAAAACTTTAGTAACTCAACTTTCAATTTTAATGATTGTTTCAACAGTTGTTATTGTTGCAACAACGATTACAGTACAACGTTTGCAAAAATCACAAAGCGATATTAAAAAAATTGGTTTACAAAGTGATTTTTTAATTGATACAAAAGAGCAAGATAATATTAATTTCAACGAAATTGACGTTAGCAACAATCTTGTAAAACATAATGAAAAATTAATTCAATTAGCAACTTTTAATCAAAATATTTTAAAAAATTCAAATAATTATTTACAACTAGTTGATAATGCATTTCATAATCATAACCTTTTTTGGCCAACTAATGTAAACAATCAAAAAATAGATGTACTAAATCTTGAAGGAAATATCGATTGA", "aa": "MKKMFLLMKNMMRLFFKNKTLVTQLSILMIVSTVVIVATTITVQRLQKSQSDIKKIGLQSDFLIDTKEQDNINFNEIDVSNNLVKHNEKLIQLATFNQNILKNSNNYLQLVDNAFHNHNLFWPTNVNNQKIDVLNLEGNID", "aa_hexdigest": "1c16f4d5b28f53c2875b6cf593ed75b6", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_UPI0002D2A8F1", "query_cov": 1.0, "subject_cov": 0.844311377245509, "identity": 0.872, "score": 228.0, "evalue": 1.97e-74, "valid": false, "uniref50_id": "UniRef50_UPI0002D2A8F1" }, "pscc": { "uniref50_id": "UniRef50_UPI0002D2A8F1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002D2A8F1" ] }, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 254267, "stop": 254911, "observations": { "mutation": [ 254449, 254488 ], "stop": [ 254449, 254488 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0029075D7D", "query_cov": 1.0, "subject_cov": 0.618421052631579, "identity": 1.0, "score": 257.0, "evalue": 2.85e-85, "gene_start": 1, "gene_end": 141, "reference_start": 1, "reference_end": 141, "reference_sequence": "MKKMFLLMKNMMRLFFKNKTLVTQLSILMIVSTVVIVATTITVQRLQKSQSDIKKIGLQSDFLIDTKEQDNINFNEIDVSNNLVKHNEKLIQLATFNQNILKNSNNYLQLVDNAFHNHNLFWPTNVNNQKIDVLNLEGNIDWNKINQLKLQTVFWFENNKENNQNFANNYDDLVYEYHNSKNQLVGFQNLDGGHILFKTPALKSANGNYETTFKFWNPTNWFLELWYS" }, "paralog": false, "identity": 0.9906542056074766, "score": 422.0, "evalue": 1.32e-152, "description": "Internal stop codon at 254449, 254488. Nonsense mutation around 254449, 254488.", "psc": { "uniref90_id": "UniRef90_UPI0029075D7D", "uniref50_id": "UniRef50_UPI0029075D7D", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0029075D7D", "UniRef:UniRef50_UPI0029075D7D" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0029075D7D", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0029075D7D" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 16378.850099999989, "isoelectric_point": 9.222992134094238 }, "id": "NKIECMJPJD_471", "locus": "GCA000828735_00437" }, { "type": "cds", "sequence": "contig_1", "start": 255190, "stop": 255642, "strand": "+", "frame": 1, "gene": "rplK", "product": "50S ribosomal protein L11", "db_xrefs": [ "COG:COG0080", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625", "GO:0070180", "KEGG:K02867", "SO:0001217", "UniParc:UPI000169E58A", "UniRef:UniRef100_A0AAC9X6B8", "UniRef:UniRef50_P47327", "UniRef:UniRef90_Q9PPU9" ], "nt": "GTGGCTCCTAAAAAGAAAGAAGTTACAAGAATCGCAAAGTTAAATCTAATTGGTGGACAAGCTAAACCTGGTCCAGCATTAGCATCAGTTGGTATTAATATGGCTGAGTTTACTAAGTCATTTAATGATAAAACTAAAGACCAAAACGGTAAGGTTATCCCAGTAATTATTACAGCTTATAAAGATAAATCATTTGATTATGTTATCAAAACTACTCCCGTGACTTTTTTACTAAAAGATGTTGCTAAAATCAAAAGTGGTGCCAAAGATCCTAAAAAGCAAACTGTTGCAACAATTTCAAAAGAACAAGCTTTAGAAATAGCTCGTTATAAATTGATTGATATGACAGCATATGATGAAGAAGCCGCTTTAAGAATGATTGCTGGTTCTGCAAAACAAATGGGAATTGTAATTGAAGGCGTAAGTGCATATAAAGAAAAGAAAGGTAACTAA", "aa": "MAPKKKEVTRIAKLNLIGGQAKPGPALASVGINMAEFTKSFNDKTKDQNGKVIPVIITAYKDKSFDYVIKTTPVTFLLKDVAKIKSGAKDPKKQTVATISKEQALEIARYKLIDMTAYDEEAALRMIAGSAKQMGIVIEGVSAYKEKKGN", "aa_hexdigest": "bdb8f6ef42a1f6941b463d1331e6de84", "start_type": "GTG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI000169E58A", "uniref100_id": "UniRef100_A0AAC9X6B8", "db_xrefs": [ "SO:0001217", "UniParc:UPI000169E58A", "UniRef:UniRef100_A0AAC9X6B8" ] }, "ips": { "uniref100_id": "UniRef100_A0AAC9X6B8", "uniref90_id": "UniRef90_Q9PPU9" }, "psc": { "uniref90_id": "UniRef90_Q9PPU9", "gene": "rplK", "product": "50S ribosomal protein L11", "uniref50_id": "UniRef50_P47327", "cog_id": "COG0080", "cog_category": "J", "kegg_orthology_id": "K02867", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625", "GO:0070180" ] }, "pscc": { "uniref50_id": "UniRef50_P47327", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47327" ], "product": "Large ribosomal subunit protein uL11" }, "genes": [ "rplK" ], "id": "NKIECMJPJD_472", "locus": "GCA000828735_00438" }, { "type": "cds", "sequence": "contig_1", "start": 255645, "stop": 256334, "strand": "+", "frame": 3, "gene": "rplA", "product": "50S ribosomal protein L1", "db_xrefs": [ "COG:COG0081", "COG:J", "GO:0000049", "GO:0003723", "GO:0003735", "GO:0006412", "GO:0006417", "GO:0015934", "GO:0019843", "KEGG:K02863", "RefSeq:WP_006688527.1", "SO:0001217", "UniParc:UPI00000C1CAF", "UniRef:UniRef100_Q9PPV0", "UniRef:UniRef50_P47328", "UniRef:UniRef90_Q9PPV0" ], "nt": "ATGGCTAAAATTTCTAAAAAATTATTTGCTGCTTATGAAGGAATTGATAAGCAAAAAGCTTACCCATTATTTGATGCAATTAAATTAGCACAAGAGAAGTCAATTACTAAATTTGATGGATCAATTAATATCGCTATTAAATTGAATTTAGATACTACTAAAGTTGAACAACAATTACGTGGGTCAATTTCATTGCCAAACGGTAATGGTAAAAATGTACGAGTTCTTGTTTTAAGTGAAGATATTACCAAAGAAGAGGCTGCTTCAGTTGGTGCTGATTATTTTGGTGGAGCAGATTACATTCAAAATATTGAAAAGATGTTAAACCAAATTGATGTAATTATTACTAACCAAAAAATGATGCCATTATTAGCTAAATTAGGAAAAGTATTGGGGCCTCGTGGACTAATGCCTAATCCAAAAATTGGAACAGTTACAAATGATGTTCTAAAAGCTGTAGAAGAATTTAAAAGAGGTCGAATTGAATACCGTACAGATACTTATGGAAATATCCATATGAGTATTGGTCGTGTTTCATTTGAAACAACTAAAATTGAAGAAAATGCTAATGCATTATTAAACTTAATTAAATCAAAAAAACCAGCGACTGTAAAGGGTCAATATATTCAAAATATTGCTGTTTCACCTACAATGGGTCCTGGAATAAAAGTTGTTATTAATAATAATTAA", "aa": "MAKISKKLFAAYEGIDKQKAYPLFDAIKLAQEKSITKFDGSINIAIKLNLDTTKVEQQLRGSISLPNGNGKNVRVLVLSEDITKEEAASVGADYFGGADYIQNIEKMLNQIDVIITNQKMMPLLAKLGKVLGPRGLMPNPKIGTVTNDVLKAVEEFKRGRIEYRTDTYGNIHMSIGRVSFETTKIEENANALLNLIKSKKPATVKGQYIQNIAVSPTMGPGIKVVINNN", "aa_hexdigest": "f400fd6cccec882aa3214c5ee0845822", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI00000C1CAF", "ncbi_nrp_id": "WP_006688527.1", "uniref100_id": "UniRef100_Q9PPV0", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1CAF", "RefSeq:WP_006688527.1", "UniRef:UniRef100_Q9PPV0" ] }, "ips": { "uniref100_id": "UniRef100_Q9PPV0", "uniref90_id": "UniRef90_Q9PPV0" }, "psc": { "uniref90_id": "UniRef90_Q9PPV0", "gene": "rplA", "product": "50S ribosomal protein L1", "uniref50_id": "UniRef50_P47328", "cog_id": "COG0081", "cog_category": "J", "kegg_orthology_id": "K02863", "go_ids": [ "GO:0000049", "GO:0003723", "GO:0003735", "GO:0006412", "GO:0006417", "GO:0015934", "GO:0019843" ] }, "pscc": { "uniref50_id": "UniRef50_P47328", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47328" ], "product": "Large ribosomal subunit protein uL1" }, "genes": [ "rplA" ], "id": "NKIECMJPJD_473", "locus": "GCA000828735_00439" }, { "type": "cds", "sequence": "contig_1", "start": 256474, "stop": 256887, "strand": "+", "frame": 1, "gene": "ecfA1", "product": "energy-coupling factor transporter ATPase", "db_xrefs": [ "EC:7.-.-.-", "GO:0005524", "GO:0006810", "GO:0009898", "GO:0016887", "GO:0042626", "GO:0043190", "SO:0001217", "UniRef:UniRef50_P47425", "UniRef:UniRef90_Q9PPV1" ], "nt": "ATGAGTCATAATGATAAAAATACTCATAATTATCAAATTTCATCATTAGATAAGATTATTAATGATTCAAGTGTTGCCATTGAATTTGAAAATGTTTATTTTGCTTATACAGAAGAACGAATGATTTTAAAAAATGTTTCATTTACAATTAATGACAATGAATATGTTTGTGTTATTGGTCATAATGGTTCTGGAAAATCAACGATATCTAAGGTTTTAACAGGATTATTAAAACCTAAAAGCGGTGTAATTAAACTTTTTGGAATTGAGATAAGTGCTGCTAATTTAAAATATTTGCGAAATAATATCGGAATTGTTTTTCAGAATCCTGATAATCAATTTGTAGGTATTACGGCTGAAGATGATATTGCTTTTGGTTTAGAAAATCGTAAGGTTCCGCCAAATAAAATGTGA", "aa": "MSHNDKNTHNYQISSLDKIINDSSVAIEFENVYFAYTEERMILKNVSFTINDNEYVCVIGHNGSGKSTISKVLTGLLKPKSGVIKLFGIEISAANLKYLRNNIGIVFQNPDNQFVGITAEDDIAFGLENRKVPPNKM", "aa_hexdigest": "97849929e4a480558733cd3aa47c7cdc", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [ "ecfA1" ], "truncated": "3-prime", "pseudogene": { "start": 256474, "stop": 257190, "observations": { "mutation": [ 256885 ], "stop": [ 256885 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PPV1", "query_cov": 1.0, "subject_cov": 0.46598639455782315, "identity": 1.0, "score": 273.0, "evalue": 1.75e-90, "gene_start": 1, "gene_end": 137, "reference_start": 1, "reference_end": 137, "reference_sequence": "MSHNDKNTHNYQISSLDKIINDSSVAIEFENVYFAYTEERMILKNVSFTINDNEYVCVIGHNGSGKSTISKVLTGLLKPKSGVIKLFGIEISAANLKYLRNNIGIVFQNPDNQFVGITAEDDIAFGLENRKVPPNKMWDIINDAAVATGIEDLLKKESLELSGGQKQRVAIASVLAINPKVIIFDESTSMLDPKGKNELKELMVSLRDVAKKTIISITHDMEEVVKADKVIVMSNGEVQYIGTPQEIFANEERLLKMQLDIPFTLKLAKTLKEKGLKIDLTLNNEELIEKICKN" }, "paralog": false, "identity": 0.9957983193277311, "score": 458.0, "evalue": 6.05e-166, "description": "Internal stop codon at 256885. Nonsense mutation around 256885.", "psc": { "uniref90_id": "UniRef90_Q9PPV1", "gene": "ecfA1", "product": "energy-coupling factor transporter ATPase", "ec_ids": [ "7.-.-.-" ], "uniref50_id": "UniRef50_P47425", "go_ids": [ "GO:0005524", "GO:0006810", "GO:0009898", "GO:0016887", "GO:0042626", "GO:0043190" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PPV1", "EC:7.-.-.-", "UniRef:UniRef50_P47425", "GO:0005524", "GO:0006810", "GO:0009898", "GO:0016887", "GO:0042626", "GO:0043190" ] }, "pscc": { "uniref50_id": "UniRef50_P47425", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47425" ], "product": "Energy-coupling factor transporter ATP-binding protein EcfA1" } }, "id": "NKIECMJPJD_474", "locus": "GCA000828735_00440" }, { "type": "cds", "sequence": "contig_1", "start": 257014, "stop": 257358, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATTATTTTTGATGAATCCACAAGTATGTTAGATCCCAAAGGAAAAAATGAATTAAAAGAATTAATGGTTTCATTGCGTGATGTTGCAAAAAAAACAATTATTTCTATTACTCATGATATGGAAGAAGTAGTTAAAGCTGATAAAGTTATTGTCATGTCAAATGGCGAAGTTCAATATATAGGTACACCACAAGAAATTTTTGCTAATGAAGAACGTTTATTAAAAATGCAATTAGATATTCCATTTACATTAAAATTAGCAAAAACCTTAAAGGAAAAGGGGTTAAAAATTGATTTAACTTTGAATAATGAGGAGTTAATTGAAAAAATATGCAAAAATTAA", "aa": "MIIFDESTSMLDPKGKNELKELMVSLRDVAKKTIISITHDMEEVVKADKVIVMSNGEVQYIGTPQEIFANEERLLKMQLDIPFTLKLAKTLKEKGLKIDLTLNNEELIEKICKN", "aa_hexdigest": "59522340d2745721e0c642bf2be8dea8", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13021.211799999983, "isoelectric_point": 5.258027458190917 }, "id": "NKIECMJPJD_475", "locus": "GCA000828735_00441" }, { "type": "cds", "sequence": "contig_1", "start": 257346, "stop": 257702, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00005.33" ], "nt": "ATGCAAAAATTAATGGATTATTTTTTCAAAAGAAAACTTAAAGTACCACGACCAATATCTAATGATATTAGTGTTCGTGTAAAAAATTTATATGCTGTTTATGATGAAAAACAAGAAAATCAATTAGTCGCATTAAATAATATTTCGTATGATTTTAAAAAAAATAAAATCTATTTTATTATTGGAAATTCTGGTTCTGGTAAATCTACGCTAGTTACTCATTTTAATGGATTAATGATCTCGCGTTATGGTTTCGTTCAAGTCGGTGATATTGTTTCTGGTGATCATTTTGATTTTGAACGCCAATTATTAGGAGTGATTGATTCATATGATAAAAAAATTATTAATTTATTATGA", "aa": "MQKLMDYFFKRKLKVPRPISNDISVRVKNLYAVYDEKQENQLVALNNISYDFKKNKIYFIIGNSGSGKSTLVTHFNGLMISRYGFVQVGDIVSGDHFDFERQLLGVIDSYDKKIINLL", "aa_hexdigest": "f4bd74347905888e95181584bc97e125", "start_type": "ATG", "rbs_motif": "AGGAG(G)/GGAGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00005.33", "name": "ABC_tran", "length": 46, "aa_cov": 0.3898305084745763, "hmm_cov": 1.0, "evalue": 7.499424457606516e-10, "score": 35.1241569519043, "start": 45, "stop": 90 } ], "seq_stats": { "molecular_weight": 13674.65609999999, "isoelectric_point": 9.424778175354007 }, "id": "NKIECMJPJD_476", "locus": "GCA000828735_00442" }, { "type": "cds", "sequence": "contig_1", "start": 257775, "stop": 258647, "strand": "+", "frame": 3, "gene": "ecfA2", "product": "Energy-coupling factor transporter ATP-binding protein EcfA2", "db_xrefs": [ "COG:COG1122", "COG:PR", "EC:3.6.3.-", "GO:0005524", "GO:0016887", "GO:0042626", "GO:0043190", "SO:0001217", "UniRef:UniRef50_Q9PPV2", "UniRef:UniRef90_Q9PPV2" ], "nt": "TTGTTTGAAGCAAATTTTAAACAAAAACCAATTAGTTTAAAATTTATTAAAACAAAAAATAATCATGAATTAATTACAAATCCTTATGTAAGAGAAAACGCTAAAATAGCAGTAGTTCGTGTTGATAAAAATGTTATATTAGAAATTAATGATAAGATGAATTATGAAGAGTTACAACGTTTTGAATTTATTAAAAAAGAAATTAAGACGAACTATCATTTATCAAAAAAAATAAAGCGGTTTAAAGAATTACGTAGACGTGTTGGTTTTGTATTCCAATTCCCTGAATACCAATTGTTTAAGGATACTATTGAAAAAGACATCATGTTTGGTCCAGTTAATTTAGGTGTAAAAAAAAGCGAAGCTAAAAAACGAGCTAAATTTTATTTAAATAAATTAGGTTTAGGAGATGATTATTTAGAACGCTCACCTTTTGGATTATCTGGAGGCCAAAAACGCCGAGTAGCAATTGCTGGAATTTTAGCAATCCAAAATGATATTTTAGTTTTTGATGAACCAACAGCTGGTTTAGATCCTGCAGGTGAACACGAAATGATGCAAATTATTTTAGATGCGAAAGCAAATAATAAAACAGTTTTTGTTATTACCCATACTATGGAACATGTGCTTGAAGTTGCTGATGAAGTGATTGTAATGGATGAAGGTGAAATTATTAAAACAGGGACACCTTATGAAATTTTTTTTGATCAACACATTATTAATTCAACTTCAATTCAAGTTCCTCGTGTAATTGCAGTTATTAATGAATTGATTAAAAAAGATCTTAAATATGAAATTCTAAAACAAAAACAACCACGCACAATTGAAGAGTTAGCAGATGCTATCATCGAATTTAAAAAAGGAGAAAAATAA", "aa": "MFEANFKQKPISLKFIKTKNNHELITNPYVRENAKIAVVRVDKNVILEINDKMNYEELQRFEFIKKEIKTNYHLSKKIKRFKELRRRVGFVFQFPEYQLFKDTIEKDIMFGPVNLGVKKSEAKKRAKFYLNKLGLGDDYLERSPFGLSGGQKRRVAIAGILAIQNDILVFDEPTAGLDPAGEHEMMQIILDAKANNKTVFVITHTMEHVLEVADEVIVMDEGEIIKTGTPYEIFFDQHIINSTSIQVPRVIAVINELIKKDLKYEILKQKQPRTIEELADAIIEFKKGEK", "aa_hexdigest": "a4c425ea8a26c6be9fbb164e1eba8262", "start_type": "TTG", "rbs_motif": null, "genes": [ "ecfA2" ], "truncated": "5-prime", "pseudogene": { "start": 257475, "stop": 258647, "observations": { "mutation": [ 257700 ], "stop": [ 257700 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PPV2", "query_cov": 1.0, "subject_cov": 0.6697459584295612, "identity": 0.993, "score": 552.0, "evalue": 3.95e-196, "gene_start": 1, "gene_end": 290, "reference_start": 144, "reference_end": 433, "reference_sequence": "MQKLMDYFFKRKLKVPRPISNDISVRVKNLYAVYDEKQENQLVALNNISYDFKKNKIYFIIGNSGSGKSTLVTHFNGLMISRYGFVQVGDIVSGDHFDFERQLLGVIDSYDKKIINLLWRNQLDQWTFLVLFSNEVNIQQARILFEANFKQKPISLKFIKTKNNHELITNPYVRENTKIAVVRVDKNVILEINDKMNYEELQRFEFIKKEIKTNYHLSKKIKRFKELRRRVGFVFQFPEYQLFKDTIEKDIMFGPVNLGVKKSEAKKRAKFYLNKLGLGDDYLERSPFGLSGGQKRRVAIAGILAIQNDILVFDEPTAGLDPAGEHEMMQIILDAKANNKTVFVITHTMEHVLEVADEVIVMDEGEIIKTGTPYEIFFDQHIINSTSIQVPRVIAVINELIKKDLKYEILKQKQPRTIEELADAIIEFKKGEK" }, "paralog": false, "identity": 0.9948717948717949, "score": 763.0, "evalue": 1.06e-281, "description": "Internal stop codon at 257700. Nonsense mutation around 257700.", "psc": { "uniref90_id": "UniRef90_Q9PPV2", "gene": "ecfA2", "product": "Energy-coupling factor transporter ATP-binding protein EcfA2", "ec_ids": [ "3.6.3.-" ], "uniref50_id": "UniRef50_Q9PPV2", "cog_id": "COG1122", "cog_category": "PR", "go_ids": [ "GO:0043190", "GO:0005524", "GO:0016887", "GO:0042626" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PPV2", "EC:3.6.3.-", "UniRef:UniRef50_Q9PPV2", "COG:COG1122", "COG:PR", "GO:0043190", "GO:0005524", "GO:0016887", "GO:0042626" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PPV2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PPV2" ], "product": "Energy-coupling factor transporter ATP-binding protein EcfA2" } }, "id": "NKIECMJPJD_477", "locus": "GCA000828735_00443" }, { "type": "cds", "sequence": "contig_1", "start": 258648, "stop": 258836, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTGCTAATGCTTATGTGTTTCGACGTTCACCAATTCATCGTTTAAATCCAGCAATTAAATTTATTTCTTTTATTTTATTAATTGCTATGATTTTTTTACCACTTGGCTTTTTTGCGCAAATGATTATTGGAATTTTTATTTTAATCGTCTTTTTTGTAGCTAAATTACCTAAGCGTACTTTATGA", "aa": "MSANAYVFRRSPIHRLNPAIKFISFILLIAMIFLPLGFFAQMIIGIFILIVFFVAKLPKRTL", "aa_hexdigest": "9960f0a70270e55dd54b2cd764d647ce", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7140.828099999996, "isoelectric_point": 11.999967765808105 }, "id": "NKIECMJPJD_478", "locus": "GCA000828735_00444" }, { "type": "cds", "sequence": "contig_1", "start": 259344, "stop": 259964, "strand": "+", "frame": 3, "gene": "ecfT", "product": "Energy-coupling factor transporter transmembrane protein EcfT", "db_xrefs": [ "COG:COG0619", "COG:H", "KEGG:K16785", "SO:0001217", "UniRef:UniRef50_A0A0C5RLA6", "UniRef:UniRef90_A0AAC9T2R8" ], "nt": "TTGTCAGGTGTAGGTGATCAATTAGGTAAGGCTCAAACCGCTTTATTTTATACACGAAGTCCTTTTGCTTTAAGTCCTATTGCTATTCAATTAGCTGTTTATATATCAATAAAAATTTTTTTAATGATAACCTTATCATCAATTCTAACAGCTACGACTTCATCTATTGAATTGACAAATGGTTTGGAAGATTTGTTAAGTCCTTTTAAAATTTTACGATTGCCTGTTGCTGAGGCTTCAATGATGATTTCAATTGCATTACGTTTTATTCCGTCATTATTAGATGAATCAAAAAGAATATTAAATGCACAAGCTTCACGTGGTGTTGATTTTAATAATGGCGGACCTATTCAAAAATTAAAATCATTAATTTCACTAGTTGTACCATTATTTTCAATTGCTTTTAAAAAAGCAGAAGATTTAGCAAATGCTATGGAAGCACGTTCTTATAACCCTCGTTATGCACGAACACGTTATCGTACTTTTCCATTAAACCTAGCAGATTATGCTTTATTTGGAATTTTGTGTATTCTCGTTGGATTTTTAATATCATTGGCCGTGATTAAATTTTATTTCACTCCATTTGGTGTATTTGAAGCAAGTGCATTGTTTGCTAAGTAA", "aa": "MSGVGDQLGKAQTALFYTRSPFALSPIAIQLAVYISIKIFLMITLSSILTATTSSIELTNGLEDLLSPFKILRLPVAEASMMISIALRFIPSLLDESKRILNAQASRGVDFNNGGPIQKLKSLISLVVPLFSIAFKKAEDLANAMEARSYNPRYARTRYRTFPLNLADYALFGILCILVGFLISLAVIKFYFTPFGVFEASALFAK", "aa_hexdigest": "d6e19310319db4f219e8adae4d14aa4f", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "ecfT" ], "truncated": "5-prime", "pseudogene": { "start": 259044, "stop": 259964, "observations": { "mutation": [ 259056, 259308 ], "stop": [ 259056, 259308 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAC9T2R8", "query_cov": 1.0, "subject_cov": 0.4703196347031963, "identity": 0.951, "score": 365.0, "evalue": 8.04e-124, "gene_start": 1, "gene_end": 206, "reference_start": 233, "reference_end": 438, "reference_sequence": "MSANAYVFRRSPIHRLNPAIKFISFILLIAMIFLPLGFFAQMIIGVFILIIFFVAKLPKKTLWNVFKSVIMLFVILLLINWMTYKDPIAIYNITDQAKVILGDKDWINGPINKNLSFSLIYNDISSTHVQNLVSNIWGGEIKNYISPEIIKKLIDKPDYNVAKFLSENNITVKKLASTFNALNQDVRLNNYYPIYGDAVLRSGKVEVPLSHLSYYMSTNLWKIEGVKYQGLILSGVGDQLGKAETALFYTRSPFALSPVAIQLAIYISIKIFLMITLSSILTATTSSIELTNGLEDLLSPFKILRLPVAEASMMISIALRFIPSLLDESKRILNAQASRGVDFNNGGMLQKLKSLISLVVPLFSIAFKKAEDLANAMEARSYNPRYARTRYRAFPLNLTDYVLFGILCILVGFLISLAVIKFYFTPFGAFEASALFAK" }, "paralog": false, "identity": 0.9281045751633987, "score": 553.0, "evalue": 4.12e-200, "description": "Internal stop codon at 259056, 259308. Nonsense mutation around 259056, 259308.", "psc": { "uniref90_id": "UniRef90_A0AAC9T2R8", "gene": "EcfT", "product": "Energy-coupling factor transporter transmembrane protein EcfT", "uniref50_id": "UniRef50_A0A0C5RLA6", "cog_id": "COG0619", "cog_category": "H", "kegg_orthology_id": "K16785", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAC9T2R8", "UniRef:UniRef50_A0A0C5RLA6", "COG:COG0619", "COG:H", "KEGG:K16785" ] }, "pscc": { "uniref50_id": "UniRef50_A0A0C5RLA6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0C5RLA6" ], "product": "Cobalt transporter" } }, "id": "NKIECMJPJD_479", "locus": "GCA000828735_00445" }, { "type": "cds", "sequence": "contig_1", "start": 260302, "stop": 260535, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCAAAAGGTTGATGATTCATTTCATGCTCAATATAATGCTACTTCTAAAACATATCAATATGTCATTAATGATTATGAATTTGATTTGTTTAGAAATAATTATGAACTTTTTGTTAATCAAAAAATTAATGATCAAAAAATATTAGAAGCATTAGAATTGTTTGTTGGCGAACATGATTTTAAATCGTTTTCAACTAGTGAATTAACAATGACAATACGTCGCATTAATTGA", "aa": "MQKVDDSFHAQYNATSKTYQYVINDYEFDLFRNNYELFVNQKINDQKILEALELFVGEHDFKSFSTSELTMTIRRIN", "aa_hexdigest": "5512a02d0d1258140d2635cae00c63f5", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9267.208299999993, "isoelectric_point": 4.943366813659667 }, "id": "NKIECMJPJD_480", "locus": "GCA000828735_00446" }, { "type": "cds", "sequence": "contig_1", "start": 260599, "stop": 260751, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01416.25" ], "nt": "ATGGTTCGTATGATCGTTGCTTCTTGTTTAGATTATGCTTTTAATAAAATAAGTTTAGCAAAAATTCATGAATTATTAATATTCCCCAAAAAGGGTGCTAGTATTAAATTAGCACCCGCTTGTGGATTATATTTGTATAAAGTCTATTATTAA", "aa": "MVRMIVASCLDYAFNKISLAKIHELLIFPKKGASIKLAPACGLYLYKVYY", "aa_hexdigest": "a3f9bc7c38e25367b1080dc57ea822bf", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01416.25", "name": "PseudoU_synth_1", "length": 51, "aa_cov": 1.0, "hmm_cov": 1.0, "evalue": 3.029147689925375e-07, "score": 26.47565269470215, "start": 1, "stop": 50 } ], "seq_stats": { "molecular_weight": 5668.886000000001, "isoelectric_point": 9.470099449157715 }, "id": "NKIECMJPJD_481", "locus": "GCA000828735_00447" }, { "type": "cds", "sequence": "contig_1", "start": 260814, "stop": 261041, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCATTAAATATTCGTTCTGCACCCCATAAAATTCTTGAAAATATTAACCATGTTTTTGATAATCAACTAAATAATCATACAATTGAAAAAGTATGTATTCTACAAGCGGGATTATCAAGTGAAAGAATTGATGAACTAAAAAATGATGTAATTAACCATTTTCATGTTGATAAGGAAAAGATTGTAATTAGATCTGGTCCTCCAATCTTTTTAGTATATGTTTGA", "aa": "MALNIRSAPHKILENINHVFDNQLNNHTIEKVCILQAGLSSERIDELKNDVINHFHVDKEKIVIRSGPPIFLVYV", "aa_hexdigest": "332312d60edbd35b9a851b5f47dbc10e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8612.853899999996, "isoelectric_point": 6.490069389343262 }, "id": "NKIECMJPJD_482", "locus": "GCA000828735_00448" }, { "type": "cds", "sequence": "contig_1", "start": 261060, "stop": 261569, "strand": "-", "frame": 1, "gene": "degV", "product": "DegV domain-containing protein UU535", "db_xrefs": [ "COG:COG1307", "COG:I", "GO:0008289", "SO:0001217", "UniRef:UniRef50_Q9PPV5", "UniRef:UniRef90_Q9PPV5" ], "nt": "ATGATTCTGCCGCTTTCTATTTTACGAAGTGATCATACTTTAATTGTAGATGATGGTATAGAATCAAAGCCAGAGCGAATTTATGAAGACATTGATAATGGATACACCTTTCAAACAAGTTGTACACCATATGGTGTTTTAATTGAAGCTATTGAACAAAAACTACAAGAATATGAAAAAATTATTTTTATTGGCATCTCTAGTGGATTTAGTAGTCAATTCAATAATGCAAAAAATTTAGAGAAAGAATATCAAGACAAATTATTTGTTGTAGACACAGAAGACTTTGGCTATTCCTTAGAACATCTAGTTTACAAAATTAAAGCAATGTTAAGCAATAATATTAGTTTTGGTGATATTTTAAAAATGATTAACAAACACCATGATTATACAAGTAGTTTTTTAGCATGTGAAAATATTACTGGCCTTGTTCGTTCTGGTCGAATCCCTAAAATCATTGGTACTATGTTAAAATTAAGTAAAGTAACACCCATAATTAAAGCTGAATGA", "aa": "MILPLSILRSDHTLIVDDGIESKPERIYEDIDNGYTFQTSCTPYGVLIEAIEQKLQEYEKIIFIGISSGFSSQFNNAKNLEKEYQDKLFVVDTEDFGYSLEHLVYKIKAMLSNNISFGDILKMINKHHDYTSSFLACENITGLVRSGRIPKIIGTMLKLSKVTPIIKAE", "aa_hexdigest": "19c0d65a05ce72f42be7037cb889438d", "start_type": "ATG", "rbs_motif": null, "genes": [ "degV" ], "truncated": "both", "pseudogene": { "start": 260757, "stop": 261644, "observations": { "mutation": [ 260816, 261062, 261590 ], "stop": [ 260816, 261062, 261590 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PPV5", "query_cov": 1.0, "subject_cov": 0.5399361022364217, "identity": 0.976, "score": 332.0, "evalue": 4.74e-113, "gene_start": 1, "gene_end": 169, "reference_start": 26, "reference_end": 194, "reference_sequence": "MKKSFLIMTDSSTTLDREWAKNNDVMILPLSILRSDHTLIVDDGIESKPERIYEDIDNGYTFQTSCTPYGALIEAIEQKLQEYEKIIFIGISSGFSSQFSNAKNLEKEYQDKLFVVDTEDFGYSLEHLVYKIKVMLTNNISFGDILKMINKHHDYTSSFLACENITGLVRSGRIPKIIGTMLKLSKVTPIIKAEWKNHRAGMALNIRSAPHKILENISHVFDNQLNNHTIEKVCILQAGLSNERIDELKNDVINHFHIDKEKIVIRSGPPIFLVYVWKGALGIQVIANIPKKHVEKNINNKLYTDIIHKQGLI" }, "paralog": false, "identity": 0.9661016949152542, "score": 565.0, "evalue": 2.84e-207, "description": "Internal stop codon at 260816, 261062, 261590. Nonsense mutation around 260816, 261062, 261590.", "psc": { "uniref90_id": "UniRef90_Q9PPV5", "gene": "DegV", "product": "DegV domain-containing protein UU535", "uniref50_id": "UniRef50_Q9PPV5", "cog_id": "COG1307", "cog_category": "I", "go_ids": [ "GO:0008289" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PPV5", "UniRef:UniRef50_Q9PPV5", "COG:COG1307", "COG:I", "GO:0008289" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PPV5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PPV5" ], "product": "DegV domain-containing protein UU535" } }, "id": "NKIECMJPJD_483", "locus": "GCA000828735_00449" }, { "type": "cds", "sequence": "contig_1", "start": 261797, "stop": 262222, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF07973.19" ], "nt": "ATGTATAAGTTTGATCAAAAACTAAATCACAGTGCTGCACATCTTTTAGCAATGGCACTAACAAAATTTTATCCTAACTTAAGCCTTGCGATTGGGCCAACGATTGATGAGGGGTTTTATTATGATTTTAATCTAAATGATCCAAATACCTCAATTACCCCTTTAGATTTATTAAAAATTGAAAAAGAAATGAAGAAAATTACTACTCAAGCTTTAACGTTTGATTATGAACAAGTTACTTATGAAAAAGCAAAGGAATTGTTTAAACACAATAAATATAAATTAGATATTATTGAACAAAATAAAAATAATTCACTTTCAATTTATCACTCAGGTAAATGGTTTGACTTATGTAAAGGCCCGCATGTACAAAACACAAAAGAGATCAAGGCAATTAAATTATTAAATATTGCAGGTTCATATTGA", "aa": "MYKFDQKLNHSAAHLLAMALTKFYPNLSLAIGPTIDEGFYYDFNLNDPNTSITPLDLLKIEKEMKKITTQALTFDYEQVTYEKAKELFKHNKYKLDIIEQNKNNSLSIYHSGKWFDLCKGPHVQNTKEIKAIKLLNIAGSY", "aa_hexdigest": "e4ee9bfdcd1cc245a42009444f54881b", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07973.19", "name": "tRNA_SAD", "length": 31, "aa_cov": 0.2127659574468085, "hmm_cov": 1.0, "evalue": 6.986476793400913e-13, "score": 43.974327087402344, "start": 107, "stop": 136 } ], "seq_stats": { "molecular_weight": 16338.599799999993, "isoelectric_point": 8.491018104553223 }, "id": "NKIECMJPJD_484", "locus": "GCA000828735_00450" }, { "type": "cds", "sequence": "contig_1", "start": 262604, "stop": 262924, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00587.30" ], "nt": "ATGACATGTCCTCATCATACATTAGTTTACTCTAATGAACTTCGTTCATATCGAAGTTTACCAATTCGTTTAAGTGAACATTCAATTTTACATCGTTATGAATCATCAGGTGGATTAACAGGCTTTGAGCGGGTTCGTGAGATGATTTTAGAAGATTGTCATGTTTTTTGTCGCTTTGATCAAATTGAACATGAAGTTATTAATGCTTTTAAAATGATTCAAGAAGCTCAAGAAGGATTGGGTATTAAAACATTTGAAATTCATTTATCTTTAAATGATCCTAATGATAAAAAAAAATATTATGATGATCCTCAAATGTGA", "aa": "MTCPHHTLVYSNELRSYRSLPIRLSEHSILHRYESSGGLTGFERVREMILEDCHVFCRFDQIEHEVINAFKMIQEAQEGLGIKTFEIHLSLNDPNDKKKYYDDPQM", "aa_hexdigest": "c361588c12017c1eb60a228f751ac608", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00587.30", "name": "tRNA-synt_2b", "length": 95, "aa_cov": 0.8773584905660378, "hmm_cov": 0.968421052631579, "evalue": 7.54065826786268e-19, "score": 64.01614379882812, "start": 1, "stop": 93 } ], "seq_stats": { "molecular_weight": 12528.072999999988, "isoelectric_point": 5.749798393249511 }, "id": "NKIECMJPJD_485", "locus": "GCA000828735_00451" }, { "type": "cds", "sequence": "contig_1", "start": 262952, "stop": 263230, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00587.30" ], "nt": "ATGTTAAAAGATCACAATATTCCTTATAAGGAGATGGTAGGAGAAGCGGCATTTTATGGTCCAAAAATTGATTTTCAGGTTAAAACTGTTTTAAATCGAATTATTACAGTTTCAACTATCCAATTAGATTTTTTATTACCAAACCGTTTTAATTTAACTTATATTAATGAAAGTAATGAACAAAGCGTACCGGTAATGATTCATATTGGTATTATTGGAACTTATGAACGTTTATTAGCAATTTTATTAGAACAAACTAAAGGTATTTTACCGCTTTGA", "aa": "MLKDHNIPYKEMVGEAAFYGPKIDFQVKTVLNRIITVSTIQLDFLLPNRFNLTYINESNEQSVPVMIHIGIIGTYERLLAILLEQTKGILPL", "aa_hexdigest": "a0975fb11fd9de7f1a799046f5c8619f", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0029CA135A", "query_cov": 0.9021739130434783, "subject_cov": 0.8217821782178217, "identity": 0.614, "score": 100.0, "evalue": 6.18e-26, "valid": false, "product": "aminoacyl--tRNA ligase-related protein", "ec_ids": [ "6.1.1.3" ], "uniref50_id": "UniRef50_Q71HR4", "kegg_orthology_id": "K01868" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00587.30", "name": "tRNA-synt_2b", "length": 73, "aa_cov": 0.7717391304347826, "hmm_cov": 0.9726027397260274, "evalue": 3.975204979724203e-16, "score": 55.148681640625, "start": 15, "stop": 85 } ], "seq_stats": { "molecular_weight": 10516.25699999999, "isoelectric_point": 6.048600959777832 }, "id": "NKIECMJPJD_486", "locus": "GCA000828735_00452" }, { "type": "cds", "sequence": "contig_1", "start": 263336, "stop": 263539, "strand": "+", "frame": 2, "gene": null, "product": "His/Gly/Thr/Pro-type tRNA ligase C-terminal domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021B27EE6" ], "nt": "GTGGATTTACGTAATGAGCGTTTATCAAAAAAAATTCGTGAAGCTCAAATTCAAAAAATCCCTTATCAAATTGTTATTGGTGATGAAGAAATTAAAAACAACAAAATGGTTACTTACCGCTGCTATGGTAGTGAAAAAACGACAACAGTTTCTATTACAGATTTTATAAATATGTTAGAAAACAAGATTAGATTAAAAAAATAA", "aa": "MDLRNERLSKKIREAQIQKIPYQIVIGDEEIKNNKMVTYRCYGSEKTTTVSITDFINMLENKIRLKK", "aa_hexdigest": "47ab5125e9666fbcb19db630a6080bb0", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001A05B1B9", "query_cov": 1.0, "subject_cov": 0.9178082191780822, "identity": 0.522, "score": 65.9, "evalue": 7.61e-13, "valid": false, "product": "His/Gly/Thr/Pro-type tRNA ligase C-terminal domain-containing protein", "uniref50_id": "UniRef50_UPI0021B27EE6" }, "pscc": { "uniref50_id": "UniRef50_UPI0021B27EE6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021B27EE6" ], "product": "His/Gly/Thr/Pro-type tRNA ligase C-terminal domain-containing protein" }, "genes": [], "id": "NKIECMJPJD_487", "locus": "GCA000828735_00453" }, { "type": "cds", "sequence": "contig_1", "start": 263600, "stop": 263761, "strand": "+", "frame": 2, "gene": "rpmG", "product": "50S ribosomal protein L33", "db_xrefs": [ "COG:COG0267", "COG:J", "GO:0003735", "GO:0005737", "GO:0005840", "GO:0006412", "GO:1990904", "RefSeq:WP_006688661.1", "SO:0001217", "UniParc:UPI0000133F9B", "UniRef:UniRef100_Q9PPV7", "UniRef:UniRef50_P47567", "UniRef:UniRef90_Q9PPV7" ], "nt": "ATGGCGATTAAACGAGGCGTTAGATTACAATGTAATGAGAGTAAAAGTATTAATTACATTACTACAAAAAATGCAAAAAATAATCCAGACAGACTTTCTTTAAATAAATTTTGTCCTAAATGTCGAAAAGTAACAACTCACGTTGAAATTAAGAAAAAATAG", "aa": "MAIKRGVRLQCNESKSINYITTKNAKNNPDRLSLNKFCPKCRKVTTHVEIKKK", "aa_hexdigest": "4e79c0d409751519124c9bf14d44291d", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI0000133F9B", "ncbi_nrp_id": "WP_006688661.1", "uniref100_id": "UniRef100_Q9PPV7", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000133F9B", "RefSeq:WP_006688661.1", "UniRef:UniRef100_Q9PPV7" ] }, "ips": { "uniref100_id": "UniRef100_Q9PPV7", "uniref90_id": "UniRef90_Q9PPV7" }, "psc": { "uniref90_id": "UniRef90_Q9PPV7", "gene": "rpmG", "product": "50S ribosomal protein L33", "uniref50_id": "UniRef50_P47567", "cog_id": "COG0267", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005737", "GO:0005840", "GO:0006412", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_P47567", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47567" ], "product": "Large ribosomal subunit protein bL33A" }, "genes": [ "rpmG" ], "id": "NKIECMJPJD_488", "locus": "GCA000828735_00454" }, { "type": "cds", "sequence": "contig_1", "start": 263772, "stop": 263873, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTGATTTCAAATTACAACAAGTTTTAAAAACAATTAAAGAACATGAAACACAAGGCGCTGACGGTATGATTTTATTTTCTAATTATAACCGTTATTGA", "aa": "MSDFKLQQVLKTIKEHETQGADGMILFSNYNRY", "aa_hexdigest": "2c521b8b6b5c6753ca72e0be4afdf170", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3906.400700000001, "isoelectric_point": 6.517920494079591 }, "id": "NKIECMJPJD_489", "locus": "GCA000828735_00455" }, { "type": "cds", "sequence": "contig_1", "start": 263904, "stop": 264239, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01321.24" ], "nt": "GTGTTTATTAATAAAGATGGTAAAGCAATTTATCTAGTGGATGCTCGTTATTATACAGCAGCGTCTGAAACTGTAAAAAACGCAAAGGTAATTTTATTAGCTCGCACACCACAAAAATCAACTTTTGATCTACTAAAAGACGCAATGGTTGAATTAAATATTACTAATGCATTAGTTGAAGCTGATTATGTAACATTAGATATTCATGAAATGTTAAAAAAATTAGTAAAAAATACTACTCCTTTTACATCAGCTACATTACGTGCTATTAAAACTGAAAAAGAACTTGAATATTTACAAAAAGCTGCTGATATTGCAGCTTTAACATGTAATTGA", "aa": "MFINKDGKAIYLVDARYYTAASETVKNAKVILLARTPQKSTFDLLKDAMVELNITNALVEADYVTLDIHEMLKKLVKNTTPFTSATLRAIKTEKELEYLQKAADIAALTCN", "aa_hexdigest": "04930c3a9d4486400493478862b1b50a", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01321.24", "name": "Creatinase_N", "length": 90, "aa_cov": 0.7657657657657657, "hmm_cov": 0.9444444444444444, "evalue": 3.381486422586493e-11, "score": 39.37331008911133, "start": 2, "stop": 86 } ], "seq_stats": { "molecular_weight": 12428.33689999998, "isoelectric_point": 7.785476112365723 }, "id": "NKIECMJPJD_490", "locus": "GCA000828735_00456" }, { "type": "cds", "sequence": "contig_1", "start": 264303, "stop": 264845, "strand": "+", "frame": 3, "gene": "pepP", "product": "XAA-PRO aminopeptidase", "db_xrefs": [ "COG:COG0006", "COG:E", "SO:0001217", "UniRef:UniRef50_Q9PPV8", "UniRef:UniRef90_Q9PPV8" ], "nt": "ATGTTAGAATTAGGTGGAGAATTAAATTCATTTGATCCAATTATTGCTTCAGGTCCAAATGGCGGATCACCACATCATCATCCAGGAAATCGTGTAATTGAAGATGGAGATATGGTAACTGTTGACATTGGTTGTACTTATAAAGGTTATTGTTCTGATATTACAAGATCTTTTATTGTTGGAAATAAAGCAAATCCACAAATGCAAGAAATTTATGATAAAGTTCTTGAATCTCAAACAGCAGGGATTAATCTATTGAGTACAAAAGTAACAGGTCAAGAAGTTGATAAAGTATGTCGCGACATTGTTGATAATTCTAAATTTAAGGGCTATTTCACACATGGAACAGGTCATGGTGTAGGTTTACAAGTACATGAATTACCAAATACTAATGCTGGTAATCCTAATAAATTGCCATTAAATGCAGTTGTTACTGTTGAACCAGGAATTTATATCCCAAATGTTGGTGGTGTAAGAATTGAAGATACAATTGTAGTAAAAGACGGACAAGCCCTGGTTTTAACAAGGTTAGCATATAAATAA", "aa": "MLELGGELNSFDPIIASGPNGGSPHHHPGNRVIEDGDMVTVDIGCTYKGYCSDITRSFIVGNKANPQMQEIYDKVLESQTAGINLLSTKVTGQEVDKVCRDIVDNSKFKGYFTHGTGHGVGLQVHELPNTNAGNPNKLPLNAVVTVEPGIYIPNVGGVRIEDTIVVKDGQALVLTRLAYK", "aa_hexdigest": "5fce2ecf2ba68143ace63825cc0f6e8a", "start_type": "ATG", "rbs_motif": null, "genes": [ "pepP" ], "truncated": "5-prime", "pseudogene": { "start": 264003, "stop": 264845, "observations": { "mutation": [ 264237 ], "stop": [ 264237 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PPV8", "query_cov": 1.0, "subject_cov": 0.5042016806722689, "identity": 0.961, "score": 362.0, "evalue": 4.52e-124, "gene_start": 1, "gene_end": 180, "reference_start": 178, "reference_end": 357, "reference_sequence": "MSDFKLQQVLKTIKEHEAQGVDGMILFCPYNRYWFLEFASSDGFVFINKDGKAIYLVDARYYTAASEAVKNAKVILLARTPQKSTFDLLKDAMVELNITNALVEADYVTLNVHEMLQKLVRKTTLFTSAALRAIKTEKELEYLQKAADIAALTCNWIREQDIIGRTELEVAMLVSKHMLELGGELNSFDPIIASGPNGGSPHHHPGNRVIEDGDMVTVDIGCTYKGYCSDITRSFIVGNKANPQMQEIYDKVLESQTAGIDLVSTKVTGQEVDKLCRDIIDNSKFNGYFTHGTGHGVGLEVHELPNTNAGNPNKLPLNAVVTVEPGIYIPNVGGVRIEDTVVVKDGQALVLTRLAYK" }, "paralog": false, "identity": 0.9392857142857143, "score": 533.0, "evalue": 9.41e-194, "description": "Internal stop codon at 264237. Nonsense mutation around 264237.", "psc": { "uniref90_id": "UniRef90_Q9PPV8", "gene": "PepP", "product": "XAA-PRO aminopeptidase", "uniref50_id": "UniRef50_Q9PPV8", "cog_id": "COG0006", "cog_category": "E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PPV8", "UniRef:UniRef50_Q9PPV8", "COG:COG0006", "COG:E" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PPV8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PPV8" ], "product": "XAA-PRO aminopeptidase" } }, "id": "NKIECMJPJD_491", "locus": "GCA000828735_00457" }, { "type": "tRNA", "sequence": "contig_1", "start": 264856, "stop": 264931, "strand": "+", "gene": "trnW", "product": "tRNA-Trp(cca)", "amino_acid": "Trp", "anti_codon": "cca", "score": 77.3, "nt": "AAGGGTATAGTTCAATTGGTAGAACAGCAGACTCCAAATCTGCGTGTTGCGGGTTCGAGTCCTGTTACCCTTGCCA", "db_xrefs": [ "SO:0000271" ], "anti_codon_pos": [ 264889, 264891 ], "id": "NKIECMJPJD_2", "locus": "GCA000828735_00458" }, { "type": "cds", "sequence": "contig_1", "start": 265056, "stop": 265430, "strand": "+", "frame": 3, "gene": "cdd", "product": "cytidine deaminase", "db_xrefs": [ "COG:COG0295", "COG:F", "EC:3.5.4.5", "GO:0004126", "GO:0005737", "GO:0008270", "GO:0008655", "GO:0009972", "KEGG:K01489", "SO:0001217", "UniRef:UniRef50_Q9PPV9", "UniRef:UniRef90_Q9PPV9" ], "nt": "ATGAAATATAATGATATTTATATGGGATTATTAGAGTTATTAAAAAAAAGTTATAGTCCTTATTCAAATTATCCAGTTGCTGCTTATGTTGATACTGATTTAGGATTTATCTCTGGTGTGAATGTTGAAAATGGATCATTTGGATTAACTTCTTGTGCTGAAAGAAATGCAATCTTTAATGCAATTGCAAATGGTGCAAAAACATTTAAAACAGTTTATATAATTACAAAAAATAATGGTGATATTGCGAGTCCTTGTGGTGCATGTCGTCAAGTGATTTTTGAATTTTTTAAAAAGGATGCAAAAATCGTCGTTTTTAACAATGATGGTACTTATAAAGAATATAATGTTGAACAATTATTACCATTTGGATGA", "aa": "MKYNDIYMGLLELLKKSYSPYSNYPVAAYVDTDLGFISGVNVENGSFGLTSCAERNAIFNAIANGAKTFKTVYIITKNNGDIASPCGACRQVIFEFFKKDAKIVVFNNDGTYKEYNVEQLLPFG", "aa_hexdigest": "720cd4c5b33b51fbfa42b29aec2ee70c", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_Q9PPV9", "query_cov": 1.0, "subject_cov": 0.9465648854961832, "identity": 1.0, "score": 253.0, "evalue": 3.65e-85, "valid": true, "gene": "cdd", "product": "cytidine deaminase", "ec_ids": [ "3.5.4.5" ], "uniref50_id": "UniRef50_Q9PPV9", "cog_id": "COG0295", "cog_category": "F", "kegg_orthology_id": "K01489", "go_ids": [ "GO:0004126", "GO:0005737", "GO:0008270", "GO:0008655", "GO:0009972" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PPV9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PPV9" ], "product": "Cytidine deaminase" }, "genes": [ "cdd" ], "id": "NKIECMJPJD_492", "locus": "GCA000828735_00459" }, { "type": "cds", "sequence": "contig_1", "start": 265690, "stop": 266427, "strand": "+", "frame": 1, "gene": "manB", "product": "Phosphomannomutase", "db_xrefs": [ "COG:COG1109", "COG:G", "SO:0001217", "UniRef:UniRef50_Q9PPW0", "UniRef:UniRef90_Q9PPW0" ], "nt": "TTGACATTAGGTTTTATTAAATATTTAGAAACGAAAAAGAATTTTAATAAAATTATTGTTATTGGCCGAGACAACCGCTTTGGCTCACGTGAAAATTTGAAACTAGTTGCTGAAATTTTTAGTAGTTTTGATTATACTGTTTATATTAATGAAGATTATGGGATGTTATCAACTCCAATTACTTCATTTTTAATTAATCAATTACAAGCAGGAGCTGGAATTATGATCACCGCTTCTCATAATCCTAAAAATTATAATGGTTTTAAAGTTTATAATGCTAATGGTGCTCAGCCATTAGTTGATGATACTAATCTCATTGAATCTTTAATGCCATCATATATAGAAGCATTGGATTTTAACTTTGAATTTAAACAAGATAATATTAGATTTTTAACAAAAGAACAAATTCAAGGTTATTTTGATGCAGTTAAAGCACAACTAATTAATACTGATCCTAATATTAAAAAACCCTTCAAAATTGTTTTTAGTGGTCATCATGGAACAACAACAAAGGACATGATTCCTTTTTTAGAATCACTTGGTTATGATATGGTTAGCGTTAGCGAACAAAATTTTGAAGATCCTAATTTTAATGATGACCCAAGTTCTAATCCAGAAGAACAATGTTCATTTGATTTAAGTGTTGAATATGCAGACAATACAAACGCAGAAATAATGATTGCATCTGATCCTGATGGAGATCGAATGGCCATTGCTGTACGTCATGAAAATTATTGA", "aa": "MTLGFIKYLETKKNFNKIIVIGRDNRFGSRENLKLVAEIFSSFDYTVYINEDYGMLSTPITSFLINQLQAGAGIMITASHNPKNYNGFKVYNANGAQPLVDDTNLIESLMPSYIEALDFNFEFKQDNIRFLTKEQIQGYFDAVKAQLINTDPNIKKPFKIVFSGHHGTTTKDMIPFLESLGYDMVSVSEQNFEDPNFNDDPSSNPEEQCSFDLSVEYADNTNAEIMIASDPDGDRMAIAVRHENY", "aa_hexdigest": "62a19b6e3e67fb18d8c69c45b9df373a", "start_type": "TTG", "rbs_motif": null, "genes": [ "manB" ], "truncated": "both", "pseudogene": { "start": 265486, "stop": 266730, "observations": { "mutation": [ 265516, 266425 ], "stop": [ 265516, 266425 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PPW0", "query_cov": 1.0, "subject_cov": 0.4438405797101449, "identity": 0.988, "score": 486.0, "evalue": 5.35e-169, "gene_start": 1, "gene_end": 245, "reference_start": 69, "reference_end": 313, "reference_sequence": "MENTNKIFNCWLNSPKIPQVIKEEYKNKTQKEIDHIFDENYRFKFGTSGVRCIYEEGTRYLNAITYTQLTLGFIEYLETKKNFNKIIVIGRDNRFGSRENLKLVAEIFSSFDYTVYINEDYGMLSTPITSFLINQLQAGAGIMITASHNPKNYNGFKVYNANGAQPLVDDTNLIESLMPSYIEALDFNFEFKQDNIRFLTKEQIQSYFDAVKAQLINTDPNIKKPFKIVFSGHHGTTTKDMIPFLESLGYDMVSVSEQNFEDPNFNDDPSSNPEEQCSFDLSVEYADNTNAEIMIASDPDGDRMAIAVRHENYWKFLTGNQTGVLIAHYLLEHKKFTKPTYIISTFISTRYPELFAKNFDCDVLYVDVGFKNHGNLIANRKKTHDLVVGFEEAIGSLPSDINNDKDSYQTASILLEMINYYYEQKLDLFTVLKKEIFAKYGNWYSKTTQFVIDGDNWKQKALIILNNLRNFEFKTVTQYTIHNIIFHEQGSYLEWKLDEYSTIKFRLSGTEPKFKVYIDLYDFSKDQKRDFYQELKIKAEDILDFLKNYLGL" }, "paralog": false, "identity": 0.9903381642512077, "score": 831.0, "evalue": 2.4e-307, "description": "Internal stop codon at 265516, 266425. Nonsense mutation around 265516, 266425.", "psc": { "uniref90_id": "UniRef90_Q9PPW0", "gene": "ManB", "product": "Phosphomannomutase", "uniref50_id": "UniRef50_Q9PPW0", "cog_id": "COG1109", "cog_category": "G", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PPW0", "UniRef:UniRef50_Q9PPW0", "COG:COG1109", "COG:G" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PPW0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PPW0" ], "product": "Phosphomannomutase" } }, "id": "NKIECMJPJD_493", "locus": "GCA000828735_00460" }, { "type": "cds", "sequence": "contig_1", "start": 266434, "stop": 266814, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02880.22" ], "nt": "TTGACTGGTAATCAAACAGGTGTTTTAATTGCACATTATTTATTAGAACATAAAAAATTTACTAAACCTACATATATTATTTCAACATTTATTTCAACACGCTATCCAGAATTATTTGCTAAAAATTTTGATTGTGATGTTTTATATGTAGATGTAGGTTTTAAAAATCATGGTAATTTAATTGCCAATCGTAAAAAAACACATGATTTGGTTGTTGGTTTTGAAGAAGCAATTGGTTCATTACCATCTGATATTAATAATGATAAAGATTCATATCAAACTGCGTCTATTTTGTTAGAAATGATTAATTATTATTATGAACAAAAACTTGATTTATTTACCGTTTTAAAAAAAGAGATTTTTGCTAAGTATGGGAATTGA", "aa": "MTGNQTGVLIAHYLLEHKKFTKPTYIISTFISTRYPELFAKNFDCDVLYVDVGFKNHGNLIANRKKTHDLVVGFEEAIGSLPSDINNDKDSYQTASILLEMINYYYEQKLDLFTVLKKEIFAKYGN", "aa_hexdigest": "e97096493f08d155c62b2f63950ee70b", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02880.22", "name": "PGM_PMM_III", "length": 106, "aa_cov": 0.8412698412698413, "hmm_cov": 0.9528301886792453, "evalue": 2.869160147135418e-14, "score": 48.79087448120117, "start": 3, "stop": 108 } ], "seq_stats": { "molecular_weight": 14510.416599999984, "isoelectric_point": 6.403219413757324 }, "id": "NKIECMJPJD_494", "locus": "GCA000828735_00461" }, { "type": "cds", "sequence": "contig_1", "start": 267363, "stop": 267548, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGAAAAAAACAAAAATATTAGTTTCAATATTAACAATCTTTTTAGCTAGTTTAAGTATGGTTGTCCTAACAACATGTTCAATGAATAAAAAAAACTATAAGAAATTTGATGAATCTAAGCAAAAATTACTTACTTTAATTAATGATTTAGATTTAGAAACTCAGAATGATATTAAAAAATGA", "aa": "MKKKTKILVSILTIFLASLSMVVLTTCSMNKKNYKKFDESKQKLLTLINDLDLETQNDIKK", "aa_hexdigest": "980341478cccddc57ef5ef7ff2153b0f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7049.4258, "isoelectric_point": 9.675173377990724 }, "id": "NKIECMJPJD_495", "locus": "GCA000828735_00462" }, { "type": "cds", "sequence": "contig_1", "start": 267597, "stop": 267857, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000456616F", "UniRef:UniRef90_UPI000456616F" ], "nt": "ATGATCATTAAGGATATAGAACAATATACTAAAGAAGTTTATGAGAAATTAGAACATTTAAAAGAAATTAAAAAAGAAAATCTAACTGAATTTAAGAAAAATAAAGATGCATTAAAAAAAATACTTCAAAACTCTGATGCTAAAGAAGTTGATTCTAATGAAATTAATAGGGCATTAAATAACATTCAAATAACAGATTTATCAACAATTATAGAAATAAAATCAGCACTTAAACGAGTTAATAATTTAAAAAAATATTAG", "aa": "MIIKDIEQYTKEVYEKLEHLKEIKKENLTEFKKNKDALKKILQNSDAKEVDSNEINRALNNIQITDLSTIIEIKSALKRVNNLKKY", "aa_hexdigest": "df1d70fef54f5261c8de518cb1abb615", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 267363, "stop": 267857, "observations": { "mutation": [ 267546 ], "stop": [ 267546 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000456616F", "query_cov": 1.0, "subject_cov": 0.524390243902439, "identity": 1.0, "score": 156.0, "evalue": 3.93e-47, "gene_start": 1, "gene_end": 86, "reference_start": 79, "reference_end": 164, "reference_sequence": "MKKKTKILVSILTIFLASLSMVVLTTCSMNKKNYKKFDESKQKLLTLINDLDLETQNDIKKWLIEQEKKFNTNYSDTEMIIKDIEQYTKEVYEKLEHLKEIKKENLTEFKKNKDALKKILQNSDAKEVDSNEINRALNNIQITDLSTIIEIKSALKRVNNLKKY" }, "paralog": false, "identity": 0.9939024390243902, "score": 312.0, "evalue": 3.99e-111, "description": "Internal stop codon at 267546. Nonsense mutation around 267546.", "psc": { "uniref90_id": "UniRef90_UPI000456616F", "uniref50_id": "UniRef50_UPI000456616F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000456616F", "UniRef:UniRef50_UPI000456616F" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000456616F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000456616F" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 10161.641599999992, "isoelectric_point": 9.0723295211792 }, "id": "NKIECMJPJD_496", "locus": "GCA000828735_00463" }, { "type": "cds", "sequence": "contig_1", "start": 267980, "stop": 268879, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0005EE675E", "UniRef:UniRef90_UPI0005EE675E" ], "nt": "TTGTTTAATAATAGTGAAATTAATAATCATTCAACGATTAAAGACATTAGAACTCAAAGAAAACAAATTGAAGATGCAATTATTAGTTTAAATCATAAAATTAAGATTTTTAAAGTGTTACAAAATAAAGAAAAAAAAGCTAAATTTGAAGAATTTAAAAGTATAAAATTGCAAATAGATAAAATTAAACGAACACAATTTAATGGCAATAGATATATAGAAATAATAAAAGAAAAAAGCGAAAAATATACGTATATTAGCCAAGATTCTGATCTAGTTGATATTCAAAATGCAATTAAGGAATTAAATAGAATAATTCAAATAAATAAAATAAAAATAAGTATTGATAATTCAACTAAATTAGACGAAATAAAAAAACAAGAATTTATAAAAGAGTGTGAGAATTTACTTGATATTAATAATTACTCAAATACATCATTAAATAATCTAAAAATAAAGATTAAAACAGCTGAAAATACAAATAATAATACAGTCAATAAAGATTTAGATAACCTTCAAAAAGAGTATGAAATAGCAAAAAATAATGTTAAAACTAAATTAATTATTCTTAAAAATAAAACTGATGCTGATTCTAAGCATGTTTTAGTTAATTCCAATAAAGTAATTAATAGTAAAATAGAAAGTATAATTAGTCAAATTGATTCTGATTATCATAAAACATACAATAATCAAAAAAAGAATATAGATGAGTGCAAAAAATATATTGATCAACTTAATAATTTGTATAAAATTAGTGCTTTATACATTGATTATTTTAGTGAAGTTAATGAAAATATTAACGCTAAAATTGATGATCAAATTATTAAAGATCAAATTTATAATTCACAAGTAATTAATCCTACAGAAGAAGTACGTAGCAAACTAGAATATTTAAATTGA", "aa": "MFNNSEINNHSTIKDIRTQRKQIEDAIISLNHKIKIFKVLQNKEKKAKFEEFKSIKLQIDKIKRTQFNGNRYIEIIKEKSEKYTYISQDSDLVDIQNAIKELNRIIQINKIKISIDNSTKLDEIKKQEFIKECENLLDINNYSNTSLNNLKIKIKTAENTNNNTVNKDLDNLQKEYEIAKNNVKTKLIILKNKTDADSKHVLVNSNKVINSKIESIISQIDSDYHKTYNNQKKNIDECKKYIDQLNNLYKISALYIDYFSEVNENINAKIDDQIIKDQIYNSQVINPTEEVRSKLEYLN", "aa_hexdigest": "0682fe097cfd69adbfeae5887eeed2e4", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 267980, "stop": 269182, "observations": { "mutation": [ 268877 ], "stop": [ 268877 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0005EE675E", "query_cov": 1.0, "subject_cov": 0.6780045351473923, "identity": 1.0, "score": 554.0, "evalue": 1.31e-196, "gene_start": 1, "gene_end": 299, "reference_start": 1, "reference_end": 299, "reference_sequence": "MFNNSEINNHSTIKDIRTQRKQIEDAIISLNHKIKIFKVLQNKEKKAKFEEFKSIKLQIDKIKRTQFNGNRYIEIIKEKSEKYTYISQDSDLVDIQNAIKELNRIIQINKIKISIDNSTKLDEIKKQEFIKECENLLDINNYSNTSLNNLKIKIKTAENTNNNTVNKDLDNLQKEYEIAKNNVKTKLIILKNKTDADSKHVLVNSNKVINSKIESIISQIDSDYHKTYNNQKKNIDECKKYIDQLNNLYKISALYIDYFSEVNENINAKIDDQIIKDQIYNSQVINPTEEVRSKLEYLNWSYYTINSQTDQFIQTYKQTLNSSLRQIKLKLFNTVMDKVFQTAREPHSNKYNLTAVLTKLNGTESDLIFKNQLTIFKGYEKLIIKFKKQNEYNETAKGIYQENGKWHFEGWIVIWNSKDNIYYYLGHSYPIVINMVEKPSN" }, "paralog": false, "identity": 0.995, "score": 779.0, "evalue": 1.16e-287, "description": "Internal stop codon at 268877. Nonsense mutation around 268877.", "psc": { "uniref90_id": "UniRef90_UPI0005EE675E", "uniref50_id": "UniRef50_UPI0005EE675E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0005EE675E", "UniRef:UniRef50_UPI0005EE675E" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0005EE675E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0005EE675E" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 35200.59670000004, "isoelectric_point": 8.9780122756958 }, "id": "NKIECMJPJD_497", "locus": "GCA000828735_00464" }, { "type": "cds", "sequence": "contig_1", "start": 268985, "stop": 269197, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATAAAGTATTTCAAACTGCACGAGAACCACATTCTAATAAATATAATTTAACAGCTGTTTTAACAAAATTGAATGGTACAGAAAGTGACTTAATTTTTAAAAACCAATTAACAATTTTTAAGGGTTATGAAAAACTAATTATTAAATTTAAAAAACAAAACGAATATAATGAGACTGCAAAAGGAATTTATCAAGAAAACGGAAAATGA", "aa": "MDKVFQTAREPHSNKYNLTAVLTKLNGTESDLIFKNQLTIFKGYEKLIIKFKKQNEYNETAKGIYQENGK", "aa_hexdigest": "f2572152491b365e7d2f7903b64d35ea", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8172.241099999997, "isoelectric_point": 9.3982816696167 }, "id": "NKIECMJPJD_498", "locus": "GCA000828735_00465" }, { "type": "cds", "sequence": "contig_1", "start": 270187, "stop": 270438, "strand": "+", "frame": 1, "gene": null, "product": "HAD family acid phosphatase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021E042A4" ], "nt": "ATGCTATTAACAGAGTATAATAGTGCAAAACACCAATTTGATGCAATGATTAAACAAAGTGGTTTTGATACAAAAAAAGTAGAAGTAAAAAAAGATTCAAATGGTAATATCACAAAGGTAACAGTTTCTAATCCTGAAAGTAATAAGGCAATTCCTGTAGTATTTATGGATATTGATGAAACAATTTTAAATAATTACGCTAATCAAAACTATCAATTATTAAATAATAAAGCTTATAATCCAAAGGATTGA", "aa": "MLLTEYNSAKHQFDAMIKQSGFDTKKVEVKKDSNGNITKVTVSNPESNKAIPVVFMDIDETILNNYANQNYQLLNNKAYNPKD", "aa_hexdigest": "e1f00a7f182acfbd779059da6dd3fe56", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0021E042A4", "query_cov": 0.8795180722891566, "subject_cov": 1.0579710144927537, "identity": 0.589, "score": 70.9, "evalue": 1.35e-14, "valid": false, "product": "HAD family acid phosphatase", "uniref50_id": "UniRef50_UPI0021E042A4" }, "pscc": { "uniref50_id": "UniRef50_UPI0021E042A4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021E042A4" ], "product": "HAD family acid phosphatase" }, "genes": [], "id": "NKIECMJPJD_499", "locus": "GCA000828735_00466" }, { "type": "cds", "sequence": "contig_1", "start": 270526, "stop": 270621, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTAACTCAAACCGTGAACAAGAAAACCAAGTCGATCCAACTATTGAAAACTTAGTTAGTGAAGGTTTAGAACGTAGTTTATTACCTAAATGA", "aa": "MFNSNREQENQVDPTIENLVSEGLERSLLPK", "aa_hexdigest": "e247dbb06042c7c4c6acca14559dd23e", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3587.9225000000006, "isoelectric_point": 4.420448112487794 }, "id": "NKIECMJPJD_500", "locus": "GCA000828735_00467" }, { "type": "cds", "sequence": "contig_1", "start": 270724, "stop": 270987, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGCCATGAATGAGCATAAAAAAGGTTATGATTTAAGTGAATTCGGTAGTGGTAATGCTGTAGTTTTAAAAACTGTAATGCGTATTGGTGATAATTTTGATGACTTTAATGATAATGCTTCAAAAGGTAAACTTAATAGTGAACGTGTAGCATTATTAAAAGAATATGGTAAATTATTTGGTAATTTTGATATAAATAATAAAGGTGTTAAATACGAAAAAAATCAAAACGGTCAAATGGTGAAATCTGATGAAACTTGA", "aa": "MNAMNEHKKGYDLSEFGSGNAVVLKTVMRIGDNFDDFNDNASKGKLNSERVALLKEYGKLFGNFDINNKGVKYEKNQNGQMVKSDET", "aa_hexdigest": "ff6469efa4e5557da389c2b1f9ffa00c", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9768.793699999995, "isoelectric_point": 6.5655515670776365 }, "id": "NKIECMJPJD_501", "locus": "GCA000828735_00468" }, { "type": "cds", "sequence": "contig_1", "start": 271326, "stop": 271751, "strand": "+", "frame": 3, "gene": "rpsL", "product": "30S ribosomal protein S12", "db_xrefs": [ "COG:COG0048", "COG:J", "GO:0000049", "GO:0003735", "GO:0006412", "GO:0015935", "GO:0019843", "KEGG:K02950", "RefSeq:WP_004026220.1", "SO:0001217", "UniParc:UPI0000134C86", "UniRef:UniRef100_Q9PPW5", "UniRef:UniRef50_P0A0H0", "UniRef:UniRef90_Q9PPW5" ], "nt": "ATGCCTACAATTGCGCAATTGATTCGTAATAAACGAGCACCTAAAGTAAAAAAAACTAAATCACCTGCTCTTTTATTTACATACAACTCATTACATAAAAAAACAACAAAAAACCCATCACCTCTAAAATCTGGTGTTTGTACTAGAGTTGGTACAATGACACCTAAAAAACCTAACTCAGCTTTACGTAAATATGCTAAAGTTCGTTTAAGTAATGGTTTTGAAGTTTTAGCTTACATTCCAGGTGAAGGACATAACTTACAAGAACACAGTGTCGTAGTTATTCGTGGGGGAAGAGTTAAAGACTTACCAGGGGTTCGTTATCATATTGTTCGTGGTGCTGGCGACGCATCAGGTGTTGAAAAACGTCGTCAACAACGTTCACTATACGGTGCTAAACGTCCTAAAAAAGAAGCATCTAAATAA", "aa": "MPTIAQLIRNKRAPKVKKTKSPALLFTYNSLHKKTTKNPSPLKSGVCTRVGTMTPKKPNSALRKYAKVRLSNGFEVLAYIPGEGHNLQEHSVVVIRGGRVKDLPGVRYHIVRGAGDASGVEKRRQQRSLYGAKRPKKEASK", "aa_hexdigest": "f39210c6f128ba87d0c2b122ab4ae94f", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0000134C86", "ncbi_nrp_id": "WP_004026220.1", "uniref100_id": "UniRef100_Q9PPW5", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000134C86", "RefSeq:WP_004026220.1", "UniRef:UniRef100_Q9PPW5" ] }, "ips": { "uniref100_id": "UniRef100_Q9PPW5", "uniref90_id": "UniRef90_Q9PPW5" }, "psc": { "uniref90_id": "UniRef90_Q9PPW5", "gene": "rpsL", "product": "30S ribosomal protein S12", "uniref50_id": "UniRef50_P0A0H0", "cog_id": "COG0048", "cog_category": "J", "kegg_orthology_id": "K02950", "go_ids": [ "GO:0000049", "GO:0003735", "GO:0006412", "GO:0015935", "GO:0019843" ] }, "pscc": { "uniref50_id": "UniRef50_P0A0H0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P0A0H0" ], "product": "Small ribosomal subunit protein uS12" }, "genes": [ "rpsL" ], "id": "NKIECMJPJD_502", "locus": "GCA000828735_00469" }, { "type": "cds", "sequence": "contig_1", "start": 271779, "stop": 272084, "strand": "+", "frame": 3, "gene": "rpsG", "product": "30S ribosomal protein S7", "db_xrefs": [ "COG:COG0049", "COG:J", "GO:0000049", "GO:0003735", "GO:0006412", "GO:0015935", "GO:0019843", "KEGG:K02992", "SO:0001217", "UniRef:UniRef50_P02359", "UniRef:UniRef90_Q9PPW6" ], "nt": "ATGAGAAAATTAAAACCACAAAAAAGACAAGTTTTAGCTGATCCAGTATATAACTCTCGTCTTGTAACTAAACTAATCAATGCTATTATGTATGATGGTAAAAAAGGATTAGCACAATCTATTATTTATTCAGCCTTTGAGATTGTTGAACAAAAGACTGGCAAACCAGCTTTAGAAGTTTTTAATAAGGCGATTGATAATGTAATGCCAATTATTGAACTTAAAGTAAGACGTGTTGGCGGTTCAAACTTTCAAGTACCAACTGAAGTAACGCCAGAACGTCGTCAGACTTTAGGTTTAAGATGA", "aa": "MRKLKPQKRQVLADPVYNSRLVTKLINAIMYDGKKGLAQSIIYSAFEIVEQKTGKPALEVFNKAIDNVMPIIELKVRRVGGSNFQVPTEVTPERRQTLGLR", "aa_hexdigest": "1f53bde9036a0009f1d11af9f19cc737", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_A0A519VYV2", "query_cov": 1.0, "subject_cov": 0.8278688524590164, "identity": 0.574, "score": 123.0, "evalue": 2.44e-34, "valid": false, "gene": "rpsG", "product": "30S ribosomal protein S7", "uniref50_id": "UniRef50_A0A4Q7IH49", "kegg_orthology_id": "K02992", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015935" ] }, "genes": [ "rpsG" ], "truncated": "3-prime", "pseudogene": { "start": 271779, "stop": 272243, "observations": { "mutation": [ 272082 ], "stop": [ 272082 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PPW6", "query_cov": 1.0, "subject_cov": 0.6516129032258065, "identity": 1.0, "score": 196.0, "evalue": 5.67e-63, "gene_start": 1, "gene_end": 101, "reference_start": 1, "reference_end": 101, "reference_sequence": "MRKLKPQKRQVLADPVYNSRLVTKLINAIMYDGKKGLAQSIIYSAFEIVEQKTGKPALEVFNKAIDNVMPIIELKVRRVGGSNFQVPTEVTPERRQTLGLRWITLYARLRHEHTMIEKLAHEIIDASNNVGAAIKKKEDTHKMAEANKAFAHLRW" }, "paralog": false, "identity": 0.9935064935064936, "score": 298.0, "evalue": 1.51e-105, "description": "Internal stop codon at 272082. Nonsense mutation around 272082.", "psc": { "uniref90_id": "UniRef90_Q9PPW6", "gene": "rpsG", "product": "30S ribosomal protein S7", "uniref50_id": "UniRef50_P02359", "cog_id": "COG0049", "cog_category": "J", "kegg_orthology_id": "K02992", "go_ids": [ "GO:0000049", "GO:0003735", "GO:0006412", "GO:0015935", "GO:0019843" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PPW6", "UniRef:UniRef50_P02359", "COG:COG0049", "COG:J", "KEGG:K02992", "GO:0000049", "GO:0003735", "GO:0006412", "GO:0015935", "GO:0019843" ] }, "pscc": { "uniref50_id": "UniRef50_P02359", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P02359" ], "product": "Small ribosomal subunit protein uS7" } }, "id": "NKIECMJPJD_503", "locus": "GCA000828735_00470" }, { "type": "cds", "sequence": "contig_1", "start": 272121, "stop": 272243, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00177.27" ], "nt": "ATGATTGAGAAATTAGCACATGAAATTATTGATGCTTCAAATAATGTAGGAGCAGCAATTAAGAAAAAAGAAGATACTCATAAAATGGCAGAAGCAAATAAAGCTTTTGCACACCTACGTTGA", "aa": "MIEKLAHEIIDASNNVGAAIKKKEDTHKMAEANKAFAHLR", "aa_hexdigest": "28c15e008dc86b5208bec9f4df89b165", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A380FBG9", "query_cov": 1.0, "subject_cov": 0.8, "identity": 0.75, "score": 64.7, "evalue": 4.05e-13, "valid": false, "product": "30S ribosomalprotein S7p (S5e)", "uniref50_id": "UniRef50_A0A645CV26" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00177.27", "name": "Ribosomal_S7", "length": 34, "aa_cov": 0.85, "hmm_cov": 1.0, "evalue": 1.0990694622670916e-14, "score": 49.83702850341797, "start": 1, "stop": 34 } ], "seq_stats": { "molecular_weight": 4445.0894, "isoelectric_point": 8.15926380157471 }, "id": "NKIECMJPJD_504", "locus": "GCA000828735_00471" }, { "type": "cds", "sequence": "contig_1", "start": 272273, "stop": 272422, "strand": "+", "frame": 2, "gene": null, "product": "GTP-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3D1ZAS3", "UniRef:UniRef90_UPI002E75BBB3" ], "nt": "ATGTCTAAAGAATTAAAATTATTTCGTAACTTTGGAATCATGGCACACATTGATGCTGGTAAAACAACAACCTCTGAACGGATTTTATATCATACAGGAAAGAATCATAAAATCGGAGAGACTCACGATGGTGCTGCAACAATGGATTGA", "aa": "MSKELKLFRNFGIMAHIDAGKTTTSERILYHTGKNHKIGETHDGAATMD", "aa_hexdigest": "9cc34fff7773c9f9f56edc1c04410a7b", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_UPI0025E4693E", "query_cov": 0.9183673469387755, "subject_cov": 0.8181818181818182, "identity": 0.8220000000000001, "score": 80.5, "evalue": 3.7e-19, "valid": false, "product": "GTP-binding protein", "uniref50_id": "UniRef50_A0A292SDT6" }, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 272276, "stop": 272521, "observations": { "mutation": [ 272420, 272480 ], "stop": [ 272420, 272480 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI002E75BBB3", "query_cov": 0.9795918367346939, "subject_cov": 0.5161290322580645, "identity": 0.833, "score": 87.4, "evalue": 1.8e-21, "gene_start": 2, "gene_end": 49, "reference_start": 4, "reference_end": 51, "reference_sequence": "MAKTNDLKLTRNFGIMAHIDAGKTTTTERILYYTGKTHKIGEVHDGAATMDWMVQEQERGVTITAAATTCFWKYPGGADNGKEYRFQIIDTPG" }, "paralog": false, "identity": 0.6444444444444445, "score": 112.0, "evalue": 3.06e-34, "description": "Internal stop codon at 272420, 272480. Nonsense mutation around 272420, 272480.", "psc": { "uniref90_id": "UniRef90_UPI002E75BBB3", "product": "GTP-binding protein", "uniref50_id": "UniRef50_A0A3D1ZAS3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI002E75BBB3", "UniRef:UniRef50_A0A3D1ZAS3" ] } }, "id": "NKIECMJPJD_505", "locus": "GCA000828735_00472" }, { "type": "cds", "sequence": "contig_1", "start": 272675, "stop": 273790, "strand": "+", "frame": 2, "gene": null, "product": "GTP dependent elongation factor G", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A346FYQ4" ], "nt": "ATGGATAAAACAGGTGCTGATTTTTATTACTCAATTGAGACAATGAAGAATCGTTTAGGTGTTAAAGCCACAGCTATTCAAATTCCAATCGGTGCTGAAGCCGATTTTGTTGGAAGTATTGATTTAATTGAAATGAAAGCTTATATTTATGATGGAAAAGCTGATGAAGAATATAAAATTGAAGATATTCCAGCTGATTACTTAACAAAAGCACAAGTAATGCGTTCACAAATGATCGATGATGTAGCTGTTTTTGATGATGAAGTGATGGAAAAATACTTATCTGGTGAAGAACTAAGTCATGAAGATATTAAAAAATGTATTCGTAAAGGAGTAATTTCAACTGAATTATACCCAGTATTATGTGGTACAGCTTTTAAAAACAAAGGTGTTAAAAAATTATTAGACGCAGTTGTTGATTTCTTACCTTCACCAATTGATGTTCCACCAATTAAAGGTGTTGATGATCATGGTAATCCAATTGAATATCATAATGATCCAAATGAACCATTTGCTGCCCTTGCCTTCAAAGTCGCAACTGATCCTTTTGTTGGACGTTTAACATATATTCGTGTATATAGTGGTAAACTTGACAAAGGTACTTATATTTACAATGCAACAAAAGATAAAAAAGAAAGAATTTCTCGTCTTGTAAAAATGCATTCAAACAACCGTGATGAGATTGATTCAATTAGTGCTGGTGATATTTGTGCTGTAATTGGATTAAAAGACACTACTACTGGTGATACAATTTGTGATGAAAAAAAACCAGTTATTTTAGAACAAATGGTATTTGCAGAACCTGTTATTTCTTTATCTGTTGAACCAAAAACTAAAGCAGATCAAGAAAAGATGAGTTTAGCTTTATCTAAATTAGCTGAAGAAGATCCAACTTTTAGAACATACACAAATGAAGAAACAGGTCAAACTATTATTGCTGGAATGGGTGAATTACATTTAGATGTTTTGGTTGATCGTATGCGTCGTGAATTTAATGTTCAAGTTAATGTAGGTGCTCCTCAAGTTAGTTATCGTGAGACATTTACTGAAGTTGCTGATGCTGAAGGTAAGTATATTAAGCAATCTGGTGGACGTGGTCAATATGGGCATGTCTGA", "aa": "MDKTGADFYYSIETMKNRLGVKATAIQIPIGAEADFVGSIDLIEMKAYIYDGKADEEYKIEDIPADYLTKAQVMRSQMIDDVAVFDDEVMEKYLSGEELSHEDIKKCIRKGVISTELYPVLCGTAFKNKGVKKLLDAVVDFLPSPIDVPPIKGVDDHGNPIEYHNDPNEPFAALAFKVATDPFVGRLTYIRVYSGKLDKGTYIYNATKDKKERISRLVKMHSNNRDEIDSISAGDICAVIGLKDTTTGDTICDEKKPVILEQMVFAEPVISLSVEPKTKADQEKMSLALSKLAEEDPTFRTYTNEETGQTIIAGMGELHLDVLVDRMRREFNVQVNVGAPQVSYRETFTEVADAEGKYIKQSGGRGQYGHV", "aa_hexdigest": "6c002e8bbb22673bb1295ca0dd903080", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI000DB1047A", "query_cov": 0.9622641509433962, "subject_cov": 0.8283062645011601, "identity": 0.555, "score": 395.0, "evalue": 1.12e-132, "valid": false, "product": "translation factor GTPase family protein", "uniref50_id": "UniRef50_A0A346FYQ4", "kegg_orthology_id": "K02355" }, "pscc": { "uniref50_id": "UniRef50_A0A346FYQ4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A346FYQ4" ], "product": "GTP dependent elongation factor G" }, "genes": [], "id": "NKIECMJPJD_506", "locus": "GCA000828735_00473" }, { "type": "cds", "sequence": "contig_1", "start": 273890, "stop": 274339, "strand": "+", "frame": 2, "gene": null, "product": "Translation elongation factor G, elongation factor G", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0F9ZJJ1" ], "nt": "TTGATTGAGGCATTAACATCAGGGCCAATTGCTGGTTATCAAACAATTGACGTTAAAGCAACTATTTTTGATGGTTCATATCATGATGTTGATTCATCTGGTATGGCTTATAAAATTGCTGCATCTCTTGCTTTTAAAGAAGCAGCAAAAGTATGCAAACCAGTTTTATTAGAACCTATTATGTCAGTTGATGTAACTACACCTGATGATTATTTTGGGACGGTAATGGGCGATATTTCAAAACGTCGTGGTGTAATTGAAGGACAAGAACAACGTGGTAATGCACAAGCTATTAAAGCTAAAGTTCCGCTATCAGAAATGTTTGGATATGCAACGGATTTACGTTCAAATACACAAGGACGTGGTCAATATATTATGCAATTCAGTCACTATGCACAAGCACCTAAGTCTGTTACTGAAGAAGTAATGGTAGCACGTGCAAAAAAATAA", "aa": "MIEALTSGPIAGYQTIDVKATIFDGSYHDVDSSGMAYKIAASLAFKEAAKVCKPVLLEPIMSVDVTTPDDYFGTVMGDISKRRGVIEGQEQRGNAQAIKAKVPLSEMFGYATDLRSNTQGRGQYIMQFSHYAQAPKSVTEEVMVARAKK", "aa_hexdigest": "d91232521edf4792bd164ecb0ea220ff", "start_type": "TTG", "rbs_motif": "AGxAG", "psc": { "uniref90_id": "UniRef90_A0A0F9ZJJ1", "query_cov": 0.9865771812080537, "subject_cov": 0.8855421686746988, "identity": 0.605, "score": 181.0, "evalue": 4.79e-56, "valid": false, "product": "Translation elongation factor G, elongation factor G", "uniref50_id": "UniRef50_A0A0F9ZJJ1" }, "pscc": { "uniref50_id": "UniRef50_A0A0F9ZJJ1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0F9ZJJ1" ], "product": "Translation elongation factor G, elongation factor G" }, "genes": [], "id": "NKIECMJPJD_507", "locus": "GCA000828735_00474" }, { "type": "cds", "sequence": "contig_1", "start": 274476, "stop": 275030, "strand": "+", "frame": 3, "gene": null, "product": "Elongation factor Tu", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0G0Z5D4" ], "nt": "ATGGCTAAAGCTAAATTTGAAAGAACAAAACCTCACGTTAACATTGGTACTATAGGCCACGTTGACCATGGTAAAACTACTTTAACAGCTGCAATTAGTACTGTACTTGCCAAAAAAGGACAAGCTATTGCACAATCATATGCTGATGTTGATAAAACACCAGAAGAAAGAGAACGTGGTATTACAATTAACGCTTCTCACGTTGAATATGAAACAAAAACACGTCACTATGCACACGTTGACTGTCCAGGACATGCCGATTATGTAAAAAATATGATTACAGGGGCAGCACAAATGGATGGAGCAATTTTAGTTATTGCTGCATCTGATGGGGTTATGGCTCAAACTAAAGAACATATTTTATTAGCACGTCAAGTTGGTGTTCCAAAAATCGTTGTTTTCTTAAACAAATGTGATTTCATGACAGATCCAGATATGCAAGATCTTGTTGAAATGGAAGTTCGTGAATTATTATCTAAATATGGATTTGATGGCGATAACACACCAGTTATTCGTGGTTCAGGTCTTAAGGCTTTAGAAGGAGATCCAGTTTGA", "aa": "MAKAKFERTKPHVNIGTIGHVDHGKTTLTAAISTVLAKKGQAIAQSYADVDKTPEERERGITINASHVEYETKTRHYAHVDCPGHADYVKNMITGAAQMDGAILVIAASDGVMAQTKEHILLARQVGVPKIVVFLNKCDFMTDPDMQDLVEMEVRELLSKYGFDGDNTPVIRGSGLKALEGDPV", "aa_hexdigest": "5766f16942088d67ef841c651a1648d5", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_UPI002A75969B", "query_cov": 0.9945652173913043, "subject_cov": 0.8883495145631068, "identity": 0.7809999999999999, "score": 294.0, "evalue": 1.86e-99, "valid": false, "product": "GTP-binding protein", "uniref50_id": "UniRef50_A0A0G0Z5D4" }, "pscc": { "uniref50_id": "UniRef50_A0A0G0Z5D4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0G0Z5D4" ], "product": "Elongation factor Tu" }, "genes": [], "id": "NKIECMJPJD_508", "locus": "GCA000828735_00475" }, { "type": "cds", "sequence": "contig_1", "start": 274847, "stop": 275227, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTCTTTTAGACCAACAATTTCAACCTCATCATTAACTTTTAATACACCACGTTCAACACGTCCAGTTACTACTGTACCACGTCCTGAAATTGTGAATACATCTTCAATTGCTAATAAGAATGGTTTGTCAGTACTACGTTCTGGTAATGGAATTCATGAATCAACTGCGTCCATTAATTCATCAATTTTTGCTTCTCAAACTGGATCTCCTTCTAAAGCCTTAAGACCTGAACCACGAATAACTGGTGTGTTATCGCCATCAAATCCATATTTAGATAATAATTCACGAACTTCCATTTCAACAAGATCTTGCATATCTGGATCTGTCATGAAATCACATTTGTTTAAGAAAACAACGATTTTTGGAACACCAACTTGA", "aa": "MSFRPTISTSSLTFNTPRSTRPVTTVPRPEIVNTSSIANKNGLSVLRSGNGIHESTASINSSIFASQTGSPSKALRPEPRITGVLSPSNPYLDNNSRTSISTRSCISGSVMKSHLFKKTTIFGTPT", "aa_hexdigest": "656585c553c3dfd335afe55298d7705b", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13426.987699999976, "isoelectric_point": 11.505882072448731 }, "id": "NKIECMJPJD_509", "locus": "GCA000828735_00476" }, { "type": "cds", "sequence": "contig_1", "start": 275256, "stop": 275660, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03144.31", "PFAM:PF03143.23" ], "nt": "ATGTTTAGAAAATCATTAGATCAAGCTGAAGCTGGTGATAATGCTGGTATTTTATTACGTGGTATTAAAAAAGAAGATGTTGAACGTGGTCAAGTACTTGTAAAACCAGGATCAATTAAACCTCACCGTACTTTTACTGCTAAAGTTTATATTCTTAAAAAAGAAGAAGGTGGACGTCATACACCTATTGTTTCAGGATACCGTCCACAATTCTATTTTAGAACAACAGATGTAACAGGTGCTATTTCATTACCTGCTGGTGTTGATTTGGTTATGCCAGGTGATGACGTTGAAATGACTGTAGAATTAATTGCTCCAGTTGCGATTGAAGATGGATCTAAATTCTCAATCCGTGAAGGTGGTAAAACTGTAGGTCATGGTAGCGTAATTAAAACAAGTAATTAA", "aa": "MFRKSLDQAEAGDNAGILLRGIKKEDVERGQVLVKPGSIKPHRTFTAKVYILKKEEGGRHTPIVSGYRPQFYFRTTDVTGAISLPAGVDLVMPGDDVEMTVELIAPVAIEDGSKFSIREGGKTVGHGSVIKTSN", "aa_hexdigest": "b9d779149b8642ada7b9e4f029200e83", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_UPI001EFCDF7C", "query_cov": 0.9776119402985075, "subject_cov": 0.8136645962732919, "identity": 0.71, "score": 201.0, "evalue": 5.59e-64, "valid": false, "product": "EF-Tu/IF-2/RF-3 family GTPase", "uniref50_id": "UniRef50_A0A966IFR0" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03144.31", "name": "GTP_EFTU_D2", "length": 33, "aa_cov": 0.2462686567164179, "hmm_cov": 1.0, "evalue": 3.0091136919835194e-09, "score": 32.804351806640625, "start": 1, "stop": 33 }, { "id": "PF03143.23", "name": "GTP_EFTU_D3", "length": 104, "aa_cov": 0.7014925373134329, "hmm_cov": 1.0, "evalue": 3.072838070112158e-39, "score": 129.16432189941406, "start": 38, "stop": 131 } ], "seq_stats": { "molecular_weight": 14496.45239999999, "isoelectric_point": 8.89097995758057 }, "id": "NKIECMJPJD_510", "locus": "GCA000828735_00477" }, { "type": "cds", "sequence": "contig_1", "start": 275826, "stop": 276161, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTCAATGAATTTGTTGAATCAAATAATGAATTAATAAAAGCTTTTGAAAAGTGGATTAATAGTTTAAATGATTTTATTAATTTTCACGAAGTTTCAGAAAAAAATTTATTAATTAGTAATCGAATTAACAATTATGTTTCAAATAAATTGCAAACAAATTTAATCGATAATGAAATGCTTGCTTGGTCTCAAAAAATTGAACATGAACAACATCGTGTAGCTAAAATTTTTATTAATTTTGAAAATTTAGCAATAAAAAATAAAGATTTAATTAATTCATATTTAAAAAATTCTTCATTAATTAAATACCAATTAGAATATGAAGAATTATGA", "aa": "MFNEFVESNNELIKAFEKWINSLNDFINFHEVSEKNLLISNRINNYVSNKLQTNLIDNEMLAWSQKIEHEQHRVAKIFINFENLAIKNKDLINSYLKNSSLIKYQLEYEEL", "aa_hexdigest": "2c45206020c139e548801ff105587d80", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13325.931799999988, "isoelectric_point": 5.354596900939941 }, "id": "NKIECMJPJD_511", "locus": "GCA000828735_00478" }, { "type": "cds", "sequence": "contig_1", "start": 276270, "stop": 276650, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAGTATCAGGATGGTATAAACTATAAAAAACAAAAAGTTTGTTTTAAAAATCAAACTGATCTTTATGTTGCATCTAAATCAAATGATCGTGCTTTACGAAAATCAGCTTATGAATCTCATTTTAAAGCGATATATGATTTACGTAATACTTTTAGTAAATTATTATATTATGAATATGTTAAACAAAACGAGTTAGCAAAATTACATAATTTTAAGGATTATATTTCTGCGGATGCTTTTAGTGATAAGGTTGATAAAAATTTTATAAATCATATTTATACACAAACAAAAAAATTTGCTAAAGGAATTAATAGATATACAAAATATCGAACATTATTTCTAAAACAACAATATCAATTAACTAAAGTTGAACCATGA", "aa": "MQYQDGINYKKQKVCFKNQTDLYVASKSNDRALRKSAYESHFKAIYDLRNTFSKLLYYEYVKQNELAKLHNFKDYISADAFSDKVDKNFINHIYTQTKKFAKGINRYTKYRTLFLKQQYQLTKVEP", "aa_hexdigest": "43a98bfe440b008153b9039c291b589d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15199.143699999977, "isoelectric_point": 9.667437171936037 }, "id": "NKIECMJPJD_512", "locus": "GCA000828735_00479" }, { "type": "cds", "sequence": "contig_1", "start": 276684, "stop": 276785, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTAGTATTGAATCTGCTAAAAACTTAACTTTAGAAGCTTTGGCATTATTAGGTAGTGAATATATAAATGTTGTACAAAGAGCGTTCAATGAGCAATGA", "aa": "MFSIESAKNLTLEALALLGSEYINVVQRAFNEQ", "aa_hexdigest": "95632836096c4ae27ffc5bc86964ba95", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3700.176200000001, "isoelectric_point": 4.493145179748535 }, "id": "NKIECMJPJD_513", "locus": "GCA000828735_00480" }, { "type": "cds", "sequence": "contig_1", "start": 276795, "stop": 277148, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCAAATAATAATAAAATTTCTGGAGCATATTCTATAAGTAACACTAAAGGCTTAGATAAAATTTTTATTTTAATGAATTATGATGAAACTTATAATTCTATTTTAACATTAGTTCATGAATTAGGGCATTCTGTACACACTTATTTTGCTAATCAATCACAAGAAGTATATAATGAATATGAAACCTTTTATGCAGAAATTGCTTCCATTACTAATGAGATCTTAATGAATTACCATTTATTAAAGAAATACGAAAATGATGATTTAATGCGTCTTTATATTTTAGATGAAATGATTAGTGGTTTTATTGCTACAACTACACGCCAAGCGATTTTTTCAAATTTTGAGTGA", "aa": "MPNNNKISGAYSISNTKGLDKIFILMNYDETYNSILTLVHELGHSVHTYFANQSQEVYNEYETFYAEIASITNEILMNYHLLKKYENDDLMRLYILDEMISGFIATTTRQAIFSNFE", "aa_hexdigest": "e2b613b70d7bf8bd1c67206ae87f0d46", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13656.192399999985, "isoelectric_point": 4.807919502258301 }, "id": "NKIECMJPJD_514", "locus": "GCA000828735_00481" }, { "type": "cds", "sequence": "contig_1", "start": 277652, "stop": 278242, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAAGAACAGAAACAAATGAAAGTTATTAAAAAATCCGATTCTTATTTTAAAAAAAATCTTACCGTTTTTAGAACACTTTTAGAACTTTTAAAAGAAAGCTCGCCATTTATCGCTGCAAGTGTTTTTACAGGACTATCATTAATTTTAATTGTTGTAATTGTTGCTCATCATAAAGATATTGGTGCTTATGCAGCAACTAGTATTAGTTATTTAACTATCTTTCAGTTAAGTTTTTTACAATTAGGAACAACCTTTGGCATTGTTTTTGCGATTTGGTCAAAAAGATCATTTAAAGATGGTAAGTATAAGTTTGAACCTACAAATGATACAGTAAATGCTGCTAGTTTTTATTCATTTATCTTTGGTTTAGTAGTTTTATTAATTTATTTTACAACCTCTTACATTTATAATCATTTTGCAAATGATCACCAAAATACAGTGATTAATCAAAGTGTAGGTAGTGCATATATTGTTTCAGGATTAGCAGTTGTTGGTTTAGCACCTTTGCGAAACTATTTATTAATGTTAATAAGATCAACCAAGTCCATTAATGTTTTATTAGCAATTGTTTTAGATTTTTGTACATGA", "aa": "MEEQKQMKVIKKSDSYFKKNLTVFRTLLELLKESSPFIAASVFTGLSLILIVVIVAHHKDIGAYAATSISYLTIFQLSFLQLGTTFGIVFAIWSKRSFKDGKYKFEPTNDTVNAASFYSFIFGLVVLLIYFTTSYIYNHFANDHQNTVINQSVGSAYIVSGLAVVGLAPLRNYLLMLIRSTKSINVLLAIVLDFCT", "aa_hexdigest": "10f5d74d56254ae19116c34b0f0234d2", "start_type": "TTG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 21966.469200000014, "isoelectric_point": 9.361599159240722 }, "id": "NKIECMJPJD_515", "locus": "GCA000828735_00482" }, { "type": "cds", "sequence": "contig_1", "start": 278480, "stop": 278689, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTATTTTGTTAATTACATTTGTGGTAATTAACTCACGTGTAGTTGGTTCATCATTATTAGATTTTCAATCAACGAGAATTCTAATGTACCAATCAATGATTTTCATACAAATGTTAAATTTAGGATTGTCAGATTTTTTATTTTATTTATATCAAAAACAAGAAGTCAGAGACTGTCGTTATCATTCAAGACAATTATTTTTTTGA", "aa": "MFILLITFVVINSRVVGSSLLDFQSTRILMYQSMIFIQMLNLGLSDFLFYLYQKQEVRDCRYHSRQLFF", "aa_hexdigest": "cb016cb9f81d4b455d57d835f32733ee", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8338.807799999997, "isoelectric_point": 9.032294654846194 }, "id": "NKIECMJPJD_516", "locus": "GCA000828735_00483" }, { "type": "cds", "sequence": "contig_1", "start": 278765, "stop": 278950, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTATACAAGTGAACAAAGTCCATCTTATATATTTTTAGAAAAGCAAGTTCCCGAACATTTCTATCAATCTTTAAGAAAAATTTTAATTAATGATGAAAAATTATTAGATATTATTACTAGTCATGTGAATATTGGTCGCCAAGAAGTTGTTAATGCTTTATCATCAAATGATAAAAATATTTGA", "aa": "MYTSEQSPSYIFLEKQVPEHFYQSLRKILINDEKLLDIITSHVNIGRQEVVNALSSNDKNI", "aa_hexdigest": "75b59ca46c62d1be09a2042c2cd66623", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7107.961000000001, "isoelectric_point": 5.4973764419555655 }, "id": "NKIECMJPJD_517", "locus": "GCA000828735_00484" }, { "type": "cds", "sequence": "contig_1", "start": 279020, "stop": 279289, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAATCCTTTTACACATCGATTTGTTGATATTTCAGCTTCAAATATTTATAGTTATTTAATTAAAAATAATGCTTATATTTTATTATCATTCTTTTGTACATTCTTTTCTTTTTCTTCTATTTTAAGTCGTTATGTAGGATTAATTGCCCGACGACAACGTCCTCCATATGTAATGATTGTTATTCAATTAGTTATGGTTGCGTTTACTTCTGGATTTGGGTTAACACACCAATCAGATCCACATTTCATTGGTTTAATGGCTTGA", "aa": "MKNPFTHRFVDISASNIYSYLIKNNAYILLSFFCTFFSFSSILSRYVGLIARRQRPPYVMIVIQLVMVAFTSGFGLTHQSDPHFIGLMA", "aa_hexdigest": "9043dbf0c57f3e119949083a14a08e28", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10218.919299999994, "isoelectric_point": 9.89423694610596 }, "id": "NKIECMJPJD_518", "locus": "GCA000828735_00485" }, { "type": "cds", "sequence": "contig_1", "start": 280146, "stop": 280268, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGACAAGTTTTTTATTAGGTAAATATAGTATTTTAGCTTATAGTGGGTATGGTTTAGGATTGTCAATTGGTTATATTATTTCAACAATTGCCATTAGTATTATTAAATTTAGTAAAGATTGA", "aa": "MTSFLLGKYSILAYSGYGLGLSIGYIISTIAISIIKFSKD", "aa_hexdigest": "361525e7f541dafe8d163a9d112d133f", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4306.066000000001, "isoelectric_point": 9.224861717224119 }, "id": "NKIECMJPJD_519", "locus": "GCA000828735_00486" }, { "type": "cds", "sequence": "contig_1", "start": 280467, "stop": 280838, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGTTTATTCAAGGGATTGGATTAGGTTTTACTGATTACCTATTTTATATTTTTCAAAAACAAACTATTCGTGATCGTCGTTACCATTCGCGCCAATTATTTATTTGTGTTTTTGGTTTATTGTTTGTTTATTTAATGATTGCAGCAATTATTTTTGGTTTTTCAATCAAACCTCTATCAGTCGTTTATGCTAAAGAACAAAATGAAGCATATATTAATTTAGATGCAGAAATACCAGATCATTTTTATATGTCAATTCGTCAAGGAATTTTAAATAATCCTCGATTAGTATACTTTTTAGGGGCACAAGCCCATATTGATCCAAGATTGATTTTAGCACACTTAACAAACCATAATCCTGAGGTATGA", "aa": "MLFIQGIGLGFTDYLFYIFQKQTIRDRRYHSRQLFICVFGLLFVYLMIAAIIFGFSIKPLSVVYAKEQNEAYINLDAEIPDHFYMSIRQGILNNPRLVYFLGAQAHIDPRLILAHLTNHNPEV", "aa_hexdigest": "a0048af0d4973416a137d6b89546aa3e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14370.663099999978, "isoelectric_point": 7.917313957214356 }, "id": "NKIECMJPJD_520", "locus": "GCA000828735_00487" }, { "type": "cds", "sequence": "contig_1", "start": 280935, "stop": 281177, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCAAATTTCAGCAGAGGTAGTTAAAAAATTATTAATTGGTGATAATACATATATTCATTTAACAATATTTGCAATTTTTTACTCATTGTCATTAACATTAATGCGTTATCGTGATCTAATTGCTAAAAAGCAACAATCACCTTTTCCAACATTATTATTGCAAGTTTTAGTAATTGCTTTTGTTGTTGGTTTTGGTGTTGATTACCAAAGTAGTGTTAAGTTTGCTGGATTATTGGCGTGA", "aa": "MQISAEVVKKLLIGDNTYIHLTIFAIFYSLSLTLMRYRDLIAKKQQSPFPTLLLQVLVIAFVVGFGVDYQSSVKFAGLLA", "aa_hexdigest": "5b26f62b5ffb1b55a7a6b8d1b0a31163", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8967.622299999997, "isoelectric_point": 9.455400657653808 }, "id": "NKIECMJPJD_521", "locus": "GCA000828735_00488" }, { "type": "cds", "sequence": "contig_1", "start": 281181, "stop": 281312, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCATTATCAATATCTAGTGCTGTAATTTTAATTATTTCTTTATTAATGTTTACGATTGTCTACTACAAGTATTTAAATAAAAACGATTATAAAGATAATCAAATTACTAATCAATATTCATTCAAATGA", "aa": "MPLSISSAVILIISLLMFTIVYYKYLNKNDYKDNQITNQYSFK", "aa_hexdigest": "76eaa0872b7fb290b79561f04429be36", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5094.936700000002, "isoelectric_point": 9.100760078430177 }, "id": "NKIECMJPJD_522", "locus": "GCA000828735_00489" }, { "type": "cds", "sequence": "contig_1", "start": 282323, "stop": 282472, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01732.22" ], "nt": "ATGACAGTTAGTAACCGTAACACAATAGGTAAAAATAATCCTACTAAATATGAAGCTTATGACTATGCAACAAAAAATTTTGATTTACATTCAGGAGCATCAGGAAGTCCAATAATTGATGACAATAATAACTATGTTGGAATTTATTGA", "aa": "MTVSNRNTIGKNNPTKYEAYDYATKNFDLHSGASGSPIIDDNNNYVGIY", "aa_hexdigest": "bc2c400d684ad381fbedb897826651b0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01732.22", "name": "Mycop_pep_DUF31", "length": 38, "aa_cov": 0.7755102040816326, "hmm_cov": 1.0, "evalue": 9.460213811858298e-12, "score": 40.740234375, "start": 12, "stop": 49 } ], "seq_stats": { "molecular_weight": 5444.821500000002, "isoelectric_point": 5.4509389877319325 }, "id": "NKIECMJPJD_523", "locus": "GCA000828735_00490" }, { "type": "cds", "sequence": "contig_1", "start": 282572, "stop": 283000, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01732.22" ], "nt": "TTGATTTCAGCAGCAAAACAAAATGCTTCTTTAACGCTTGGTCAATACGATTTAGAATCCCAATCCCAAGATACAAGAGCTTTTTTTAATGAAAATTTATCATTTAAATCCGCTAAAAAAACTTATAATCCACAATACTATGTAAATTATAATCCAGTAGGCACTTTTTCAATTTTGCCTGTTGGATTAAAAAACCAACTAAAAAATTATGGTATTAAAAACCCAGCCGCTTTAGCTTCTTTAAATAATTTAACAACTGCTGTTGATTTAGCTATTATCAAAATCACACCAGATATCTTACAAAAAAATAATATTGATGCTTTGAGAAATTTTGATAAAGAAGTAGGTATTGGTTTTGGTAATACTATTAGCAATCATCAAAGGTTTTATGCCGCAGGATATCCACATGATGAAAAATATAGTCGTTGA", "aa": "MISAAKQNASLTLGQYDLESQSQDTRAFFNENLSFKSAKKTYNPQYYVNYNPVGTFSILPVGLKNQLKNYGIKNPAALASLNNLTTAVDLAIIKITPDILQKNNIDALRNFDKEVGIGFGNTISNHQRFYAAGYPHDEKYSR", "aa_hexdigest": "d873a9961a3f7dbdcac5d8407ade353e", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01732.22", "name": "Mycop_pep_DUF31", "length": 138, "aa_cov": 0.9014084507042254, "hmm_cov": 0.8913043478260869, "evalue": 5.3589077251577784e-08, "score": 28.398038864135742, "start": 6, "stop": 133 } ], "seq_stats": { "molecular_weight": 15870.644699999975, "isoelectric_point": 9.246652030944823 }, "id": "NKIECMJPJD_524", "locus": "GCA000828735_00491" }, { "type": "cds", "sequence": "contig_1", "start": 283079, "stop": 283306, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTCTTATTACAGGAATTAGTATACCTGTAATAACAACAAGTTGTTCTCTAATTGATTTGTTCAAAAGTAATAATGAGAAACAAAATGAATTTACTCATGGTAAACTAAAATCTAATCTTGATTACAATGTAAGTACTGCTTATTTAAATAAAAGATCTATTGCTTTAAAATTCACAATACCTTTAGATAAAAATCAAGCTATTAATATTTTTGGAACAGGATGA", "aa": "MILITGISIPVITTSCSLIDLFKSNNEKQNEFTHGKLKSNLDYNVSTAYLNKRSIALKFTIPLDKNQAINIFGTG", "aa_hexdigest": "e8f7a4ba1b528aee928785f56c5757e0", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8318.532799999995, "isoelectric_point": 9.251745033264157 }, "id": "NKIECMJPJD_525", "locus": "GCA000828735_00492" }, { "type": "cds", "sequence": "contig_1", "start": 283456, "stop": 284217, "strand": "-", "frame": 3, "gene": "fepD", "product": "Ferric anguibactin transport system permerase protein", "db_xrefs": [ "COG:COG0609", "COG:P", "SO:0001217", "UniRef:UniRef50_Q9PPX3", "UniRef:UniRef90_Q9PPX3" ], "nt": "ATGCAAATTTTTTTATCAGGTTTTATTTTAGGATTTTGTGCATATTTAATTCAAAAATTAACAAAAAACCGCTTCAGTGATACATCAATTATGGGAATTAGTAGTGTTAATATTTTAGGAGTAATTATTATTGCATTTCAGGTTGATTTTAATAATTTTAGTGCTGATAAATTATCAACATTAGAGCATACTGAACCAATAATATTTTTTATAGCACCAATTATTTTATGTATGCTCTATTATTTTACATGCAAAGAACAAAGTAGTTTTAATTATAAAAAAATGCTGATTTCAGGAGTTATTGTCAATTTTTTAAGCGTTGCATTAGGAGCAAGTATTGCTAAAAATTTACCTAAATTAGCTAATGCTTACGTTTCAAGATACACATATGGTAGTATTGAACCACAACAAGAGTCTTTTTTTATCGCTTTAGTTTTAATTATTATAGGTCTATTAATAGTTTTATTTAATTTTAAAAAAATTCAAATAGTTTCATCTAATCAAGACTTAGCTAATCAATTAGGAATCAACGTTAAATTAACTTCTGGACTTCTTTTAGTTGCAATTTGTTTAATGGTAGGAGCATCATATAGTTTGAATGGAAATTTGATATTTATAGGATTAATGGCTGGAAACATGGGTTCTGTTATTAGTAACAACCGATTTAAATCCGCTGCTATTAGTTCAGGATGTTGTGGGTCATTAATATATATGTTGTCTTTTTTATTATTTATTAAAATTTTAAATTTTGATAGTCAATGA", "aa": "MQIFLSGFILGFCAYLIQKLTKNRFSDTSIMGISSVNILGVIIIAFQVDFNNFSADKLSTLEHTEPIIFFIAPIILCMLYYFTCKEQSSFNYKKMLISGVIVNFLSVALGASIAKNLPKLANAYVSRYTYGSIEPQQESFFIALVLIIIGLLIVLFNFKKIQIVSSNQDLANQLGINVKLTSGLLLVAICLMVGASYSLNGNLIFIGLMAGNMGSVISNNRFKSAAISSGCCGSLIYMLSFLLFIKILNFDSQ", "aa_hexdigest": "ebbdaa313737822ba2d5d3311c1fad52", "start_type": "ATG", "rbs_motif": null, "genes": [ "fepD" ], "truncated": "both", "pseudogene": { "start": 283378, "stop": 284403, "observations": { "mutation": [ 283458, 284271, 284385 ], "stop": [ 283458, 284271, 284385 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PPX3", "query_cov": 1.0, "subject_cov": 0.7419354838709677, "identity": 0.9209999999999999, "score": 446.0, "evalue": 4.87e-156, "gene_start": 1, "gene_end": 253, "reference_start": 63, "reference_end": 315, "reference_sequence": "MINLSTWNKTYSFKNPLKYQNKLAYLKTIITVAISIIVFIITLAWTYDFNFKQNNIALVYIVMQIFLSGFVLGFCAYFIQKLTKNRFGDTSIMGISSVNILGVIIIAFQVDFNNFSIDKLTTLQRTEPLIFFIAPIILCSIYYFTCKEESNFNYKKMLISGVIVNFLSVALGSSIAKNLPKLANAYISRYTYGSIEPQQESFFIALVLIIIGFLIIMFNFKKIQIVSSNQDLANQLGINVKLISGLLLVAICLMVGASYSLNGNLIFIGLMAGNMGSVISNNRFKSAAISSGSCGALIYMLSFLLFIKILNFDSQWLNLAIPLLISPYFIYIIVKKNKSDL" }, "paralog": false, "identity": 0.9032258064516129, "score": 603.0, "evalue": 8.68e-221, "description": "Internal stop codon at 283458, 284271, 284385. Nonsense mutation around 283458, 284271, 284385.", "psc": { "uniref90_id": "UniRef90_Q9PPX3", "gene": "FepD", "product": "Ferric anguibactin transport system permerase protein", "uniref50_id": "UniRef50_Q9PPX3", "cog_id": "COG0609", "cog_category": "P", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PPX3", "UniRef:UniRef50_Q9PPX3", "COG:COG0609", "COG:P" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PPX3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PPX3" ], "product": "Ferric anguibactin transport system permerase protein" } }, "id": "NKIECMJPJD_526", "locus": "GCA000828735_00493" }, { "type": "cds", "sequence": "contig_1", "start": 284405, "stop": 284818, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTGTTTCAAGTGTTCTAATGATTATTTCTACTATTATTTTATTATTTTTATCTCCTTATTTAAATATTATTAGAAAAGATTATATACTTGCTAAATCATTGGGGATTAAAGTCAATCTAATTTATTGGTTAGTGGCTATTTGTGTGGTAACTATTACTATATCATCATCTATATTATTGGGAATAATTGCTTTATTAGGAGTAATTGTTGGAATTATTATTCAAACAATTTTTAAAAAAACACACGTTTTATTACTAATGATACTAGCAGGACTTTTTGGTAGTGGAATCTTATCATTTTCAAGTTACATTAATGAATATATTCCTAGTGCCCGTGAAATGATTATTTGTGTTTTTGCTATACCAGTATTTGCTTACATTCTAAGTAAGAGAAAAGGATTTATTAAATAA", "aa": "MIVSSVLMIISTIILLFLSPYLNIIRKDYILAKSLGIKVNLIYWLVAICVVTITISSSILLGIIALLGVIVGIIIQTIFKKTHVLLLMILAGLFGSGILSFSSYINEYIPSAREMIICVFAIPVFAYILSKRKGFIK", "aa_hexdigest": "08dd53b31024b839991b78d2f9fb92f4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15137.601999999988, "isoelectric_point": 9.780579185485838 }, "id": "NKIECMJPJD_527", "locus": "GCA000828735_00494" }, { "type": "cds", "sequence": "contig_1", "start": 284864, "stop": 285460, "strand": "-", "frame": 2, "gene": "fepD", "product": "Iron ABC transporter permease", "db_xrefs": [ "COG:COG0609", "COG:P", "SO:0001217", "UniRef:UniRef50_A0AAC9T065", "UniRef:UniRef90_A0AAC9T065" ], "nt": "ATGAATAAAATAAAAAATGTTAAATCTTCGTTTCTATATTTTAAAAAAATGCATAATTTAAAATCTTTTAACAAAAAAAGAAATAGATTAAAAGGCATACAAATCATATTTATTGTTTTTCTAATAATCTTTTGTTCATTATTTTTCTTAATTGATTTATTTTTTACAGGTAATCATTTGAACGTCATTAAAAGAATGTTTGAAAATAGTTCTCTTTCAAATTCAACTTATATTTTTGTTCCTGTTGCTAATATAATTACAGGATTTAGTTTAGGAATTGGTTCAATTTCTATCCAAATTACTTCTAAAAATATTTTAGCTGGACCGTCAACACTTGGTTTTACACCAATGGCAATATTAGCATCAACCATTTCATTTATTATTACTTCAAGTGGTATTTTTTCAACAATTTTAATTTACTGTTTAGGTTTAATTTTTTCATTTATTATAATAGGTGTTAATTTTATATTAGTGAGAAATAATTTTTTAGAAAATAGTTTTAAACCTATTTTAGTTGCTTTTGGGATTGGAGCATTAGTAACGGGAATTAATATTGTTTTAATTGCAACACATGACAATTTAAAAATTGTGGGATGA", "aa": "MNKIKNVKSSFLYFKKMHNLKSFNKKRNRLKGIQIIFIVFLIIFCSLFFLIDLFFTGNHLNVIKRMFENSSLSNSTYIFVPVANIITGFSLGIGSISIQITSKNILAGPSTLGFTPMAILASTISFIITSSGIFSTILIYCLGLIFSFIIIGVNFILVRNNFLENSFKPILVAFGIGALVTGINIVLIATHDNLKIVG", "aa_hexdigest": "f617b5365954100dc95772a3e5fe86de", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [ "fepD" ], "truncated": "5-prime", "pseudogene": { "start": 284561, "stop": 285460, "observations": { "mutation": [ 284866 ], "stop": [ 284866 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAC9T065", "query_cov": 1.0, "subject_cov": 0.5641025641025641, "identity": 0.995, "score": 358.0, "evalue": 2.54e-122, "gene_start": 1, "gene_end": 198, "reference_start": 1, "reference_end": 198, "reference_sequence": "MNKIKNAKSSFLYFKKMHNLKSFNKKRNRLKGIQIIFIVFLIIFCSLFFLIDLFFTGNHLNVIKRMFENSSLSNSTYIFVPVANIITGFSLGIGSISIQITSKNILAGPSTLGFTPMAILASTISFIITSSGIFSTILIYCLGLIFSFIIIGVNFILVRNNFLENSFKPILVAFGIGALVTGINIVLIATHDNLKIVGWWRFITINNTLINNYRMIVSSVLMIISTIILLFLSPYLNIIRKDYILAKSLGIKVNLIYWLVAICVVTITISSSILLGIIALLGVIVGIIIQTIFKKTHVLLLMILAGLFGSGILSFSSYINEYIPSAREMIICVFAIPVFAYILSKRKGFIK" }, "paralog": false, "identity": 0.9933110367892977, "score": 565.0, "evalue": 1.76e-206, "description": "Internal stop codon at 284866. Nonsense mutation around 284866.", "psc": { "uniref90_id": "UniRef90_A0AAC9T065", "gene": "FepD", "product": "Iron ABC transporter permease", "uniref50_id": "UniRef50_A0AAC9T065", "cog_id": "COG0609", "cog_category": "P", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAC9T065", "UniRef:UniRef50_A0AAC9T065", "COG:COG0609", "COG:P" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAC9T065", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T065" ], "product": "Iron ABC transporter permease" } }, "id": "NKIECMJPJD_528", "locus": "GCA000828735_00495" }, { "type": "tRNA", "sequence": "contig_1", "start": 285654, "stop": 285728, "strand": "+", "gene": "trnN", "product": "tRNA-Asn(gtt)", "amino_acid": "Asn", "anti_codon": "gtt", "score": 83.5, "nt": "GGCTACATAGCTCAGCGGTAGAGCAACCGGCTGTTAACCGGTTGGTCGTAGGTTCGATCCCTACTGTAGCCGCCA", "db_xrefs": [ "SO:0000257" ], "anti_codon_pos": [ 285686, 285688 ], "id": "NKIECMJPJD_3", "locus": "GCA000828735_00496" }, { "type": "tRNA", "sequence": "contig_1", "start": 285730, "stop": 285806, "strand": "+", "gene": "trnE", "product": "tRNA-Glu(ttc)", "amino_acid": "Glu", "anti_codon": "ttc", "score": 59.0, "nt": "GGCCTGTTGGTGAAGTGACTGAACACACACGCCTTTCACGCGTGCATTCACGGGTTTGAATCCCGTACAGGTCACCA", "db_xrefs": [ "SO:0000259" ], "anti_codon_pos": [ 285764, 285766 ], "id": "NKIECMJPJD_4", "locus": "GCA000828735_00497" }, { "type": "tRNA", "sequence": "contig_1", "start": 285853, "stop": 285928, "strand": "+", "gene": "trnV", "product": "tRNA-Val(tac)", "amino_acid": "Val", "anti_codon": "tac", "score": 85.0, "nt": "GGAGTGCTAGCTCAGTTGGGAGAGCATTTGCCTTACAAGCAAAGGGTCAGGGGTTCGAGTCCCTTGCACTCCACCA", "db_xrefs": [ "SO:0000273" ], "anti_codon_pos": [ 285886, 285888 ], "id": "NKIECMJPJD_5", "locus": "GCA000828735_00498" }, { "type": "tRNA", "sequence": "contig_1", "start": 285930, "stop": 286005, "strand": "+", "gene": "trnT", "product": "tRNA-Thr(tgt)", "amino_acid": "Thr", "anti_codon": "tgt", "score": 82.3, "nt": "GCCGACTTAGCTCAATAGGTAGAGCAACTGACTTGTAATCAGTAGGTTGTGGGTTCAATTCCTATAGTCGGCACCA", "db_xrefs": [ "SO:0000270" ], "anti_codon_pos": [ 285963, 285965 ], "id": "NKIECMJPJD_6", "locus": "GCA000828735_00499" }, { "type": "tRNA", "sequence": "contig_1", "start": 286077, "stop": 286152, "strand": "+", "gene": "trnK", "product": "tRNA-Lys(ctt)", "amino_acid": "Lys", "anti_codon": "ctt", "score": 72.1, "nt": "GCTCTGTTAGCTCAGTAGGTAGAGCAACTGACTCTTAATCAGTGGGTCGCGGGTTCGAAACCCTCACAGAGCACCA", "db_xrefs": [ "SO:0000265" ], "anti_codon_pos": [ 286110, 286112 ], "id": "NKIECMJPJD_7", "locus": "GCA000828735_00500" }, { "type": "tRNA", "sequence": "contig_1", "start": 286157, "stop": 286240, "strand": "+", "gene": "trnL", "product": "tRNA-Leu(tag)", "amino_acid": "Leu", "anti_codon": "tag", "score": 63.9, "nt": "GCGCTTGTGGCGGAATGGCAGACGCGCTAGACTTAGGATCTAGTACTTTGCGGTGTAAGAGTTCAAGTCTCTTCAAGCGCACCA", "db_xrefs": [ "SO:0000264" ], "anti_codon_pos": [ 286184, 286186 ], "id": "NKIECMJPJD_8", "locus": "GCA000828735_00501" }, { "type": "cds", "sequence": "contig_1", "start": 286388, "stop": 286501, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAAAAGCTGAATTAGTTAAAGAATTAAGAATAAGAACACAAGCTAGTATGAGTGAATGTATTAAAGCTTTAGATGCGTCAGAAAATGATATTGAAAAAGCAATAGTATGA", "aa": "MTKAELVKELRIRTQASMSECIKALDASENDIEKAIV", "aa_hexdigest": "acf2e89877ede61234ba62f87fe98da7", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4135.7818, "isoelectric_point": 5.1446336746215815 }, "id": "NKIECMJPJD_529", "locus": "GCA000828735_00502" }, { "type": "cds", "sequence": "contig_1", "start": 286568, "stop": 286981, "strand": "+", "frame": 2, "gene": "tsf", "product": "translation elongation factor Ts", "db_xrefs": [ "COG:COG0264", "COG:J", "GO:0003746", "GO:0005737", "GO:0006414", "SO:0001217", "UniRef:UniRef50_Q9PPX5", "UniRef:UniRef90_Q9PPX5" ], "nt": "TTGGCCAAAAAAATAAATAATAAAGCAATTCTTATTGAGGTTAATTGTCAAACTGACTTTGTTGCTAAGAATGAAAATTTTTTAGCTTATGCTAATCAAATTTTAGAAGAAGCATTAGCTAAGGTTGAAAATAAAGAAGATTTTGATAAATTAATTATAAATGGAAAACCAATTGCTGAATCAGGATTAGATTTAACAGCATATATTGGTGAAAAAATCGTTTTTAGACGTGGAGAAATTTTAAAAGCTAATGATGACCAAACTTTAGGTGTTTATACTCACAATAACAACAGAGTTGCTGCTATTATTTTAGTTGATGGAAAAGTTGAAGATGAAGTTGTTCGAAATGTAGCAATGCATGCTGCAGCAATGCGTCCACGTTATTTAAATGAAAAAGTTGTTGATAAGTTATGA", "aa": "MAKKINNKAILIEVNCQTDFVAKNENFLAYANQILEEALAKVENKEDFDKLIINGKPIAESGLDLTAYIGEKIVFRRGEILKANDDQTLGVYTHNNNRVAAIILVDGKVEDEVVRNVAMHAAAMRPRYLNEKVVDKL", "aa_hexdigest": "0d771586994ba3e658224ddfeb242752", "start_type": "TTG", "rbs_motif": null, "genes": [ "tsf" ], "truncated": "both", "pseudogene": { "start": 286388, "stop": 287263, "observations": { "mutation": [ 286499, 286979 ], "stop": [ 286499, 286979 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PPX5", "query_cov": 1.0, "subject_cov": 0.47079037800687284, "identity": 0.993, "score": 265.0, "evalue": 1.73e-87, "gene_start": 1, "gene_end": 137, "reference_start": 61, "reference_end": 197, "reference_sequence": "MTKAELVKELRIRTQASMSECIKALDASENDIEKAIVWLRENGAIKAANKLKNAATDGVVLAKKINNKAILIEVNCQTDFVAKNENFLAYANQILEEALAKVENKEDFDKLIINGKPIAESGLDLTAYIGEKIVFRRGEILKANDDQTLGVYTHNNNRVAAIILVDGKVEDEVVRNVAMHAAAMRPRYLNEKVVDKLWLAKEREIIVNQLEHEGKPAAFAAKIIDGRLNKILKENCLVDQSYFKQPELTIEKYLKNNNAVAVGYFSYEVGEGIEKAPQMSFADEVAAQMKK" }, "paralog": false, "identity": 0.993127147766323, "score": 554.0, "evalue": 4.63e-204, "description": "Internal stop codon at 286499, 286979. Nonsense mutation around 286499, 286979.", "psc": { "uniref90_id": "UniRef90_Q9PPX5", "gene": "tsf", "product": "translation elongation factor Ts", "uniref50_id": "UniRef50_Q9PPX5", "cog_id": "COG0264", "cog_category": "J", "go_ids": [ "GO:0003746", "GO:0005737", "GO:0006414" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PPX5", "UniRef:UniRef50_Q9PPX5", "COG:COG0264", "COG:J", "GO:0003746", "GO:0005737", "GO:0006414" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PPX5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PPX5" ], "product": "Elongation factor Ts" } }, "id": "NKIECMJPJD_530", "locus": "GCA000828735_00503" }, { "type": "cds", "sequence": "contig_1", "start": 287347, "stop": 287508, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCGAAAACAACGAATTGTAATTAAAATTTCTGGAGCTTGCTTAAAACAAAATGATAGTTCAATAATTGATTTTATAAAGATTAATGATTTAGCAGAACAAATTGAAAAAATTTCTAAGAAATATATTGTATCAATTGTTTTAGGTGGTGGCAATATTTGA", "aa": "MRKQRIVIKISGACLKQNDSSIIDFIKINDLAEQIEKISKKYIVSIVLGGGNI", "aa_hexdigest": "e2ba862d4e64e41de171261b71c8e887", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5902.968799999999, "isoelectric_point": 9.579889106750485 }, "id": "NKIECMJPJD_531", "locus": "GCA000828735_00504" }, { "type": "cds", "sequence": "contig_1", "start": 287536, "stop": 288054, "strand": "+", "frame": 1, "gene": "pyrH", "product": "UMP kinase", "db_xrefs": [ "COG:COG0528", "COG:F", "EC:2.7.4.22", "GO:0005524", "GO:0005737", "GO:0006221", "GO:0006225", "GO:0015949", "GO:0033862", "GO:0044210", "KEGG:K09903", "SO:0001217", "UniRef:UniRef50_Q9PPX6", "UniRef:UniRef90_Q9PPX6" ], "nt": "ATGGATCGTAATTTAGCTGATAATATGGGTATGATGGCAACTATTATAAATGGGTTAGCTTTAGAAAATGCTTTAAATCATTTGAATGTTAACACTATTGTTTTATCTGCAATTAAATGTGATAAATTAGTTCATGAATCATCTGCTAATAATATAAAAAAAGCTATTGAAAAAGAACAAGTAATGATTTTTGTGGCTGGAACAGGATTTCCATATTTTACAACTGATTCGTGTGCAGCAATTAGAGCTGCTGAAACAGAATCTAGTATAATTTTAATGGGCAAAAATGGTGTTGATGGTGTCTATGATAGTGATCCAAAAATTAATCCTAATGCACAATTTTATGAACATATTACATTTAATATGGCCCTTACTCAAAATTTAAAAGTTATGGATGCAACAGCTTTAGCTTTGTGTCAAGAAAATAATATAAATTTATTAGTTTTTAATATTGATAAACCAAATGCAATTGTTGATGTTTTAGAGAAAAAAAATAAATACACAATTGTTTCAAAATAA", "aa": "MDRNLADNMGMMATIINGLALENALNHLNVNTIVLSAIKCDKLVHESSANNIKKAIEKEQVMIFVAGTGFPYFTTDSCAAIRAAETESSIILMGKNGVDGVYDSDPKINPNAQFYEHITFNMALTQNLKVMDATALALCQENNINLLVFNIDKPNAIVDVLEKKNKYTIVSK", "aa_hexdigest": "ffcfdefa481a42ef37457fc7531f6d56", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "pyrH" ], "truncated": "5-prime", "pseudogene": { "start": 287347, "stop": 288054, "observations": { "mutation": [ 287506 ], "stop": [ 287506 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PPX6", "query_cov": 1.0, "subject_cov": 0.671875, "identity": 1.0, "score": 338.0, "evalue": 3.97e-116, "gene_start": 1, "gene_end": 172, "reference_start": 85, "reference_end": 256, "reference_sequence": "MGSSHHHHHHSSGLVPRGSHMMRKQRIVIKISGACLKQNDSSIIDFIKINDLAEQIEKISKKYIVSIVLGGGNIWRGSIAKELDMDRNLADNMGMMATIINGLALENALNHLNVNTIVLSAIKCDKLVHESSANNIKKAIEKEQVMIFVAGTGFPYFTTDSCAAIRAAETESSIILMGKNGVDGVYDSDPKINPNAQFYEHITFNMALTQNLKVMDATALALCQENNINLLVFNIDKPNAIVDVLEKKNKYTIVSK" }, "paralog": false, "identity": 0.9957446808510638, "score": 454.0, "evalue": 2.75e-164, "description": "Internal stop codon at 287506. Nonsense mutation around 287506.", "psc": { "uniref90_id": "UniRef90_Q9PPX6", "gene": "pyrH", "product": "UMP kinase", "ec_ids": [ "2.7.4.22" ], "uniref50_id": "UniRef50_Q9PPX6", "cog_id": "COG0528", "cog_category": "F", "kegg_orthology_id": "K09903", "go_ids": [ "GO:0005524", "GO:0005737", "GO:0006221", "GO:0006225", "GO:0015949", "GO:0033862", "GO:0044210" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PPX6", "EC:2.7.4.22", "UniRef:UniRef50_Q9PPX6", "COG:COG0528", "COG:F", "KEGG:K09903", "GO:0005524", "GO:0005737", "GO:0006221", "GO:0006225", "GO:0015949", "GO:0033862", "GO:0044210" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PPX6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PPX6" ], "product": "Uridylate kinase" } }, "id": "NKIECMJPJD_532", "locus": "GCA000828735_00505" }, { "type": "cds", "sequence": "contig_1", "start": 288132, "stop": 288623, "strand": "+", "frame": 3, "gene": "frr", "product": "ribosome recycling factor", "db_xrefs": [ "COG:COG0233", "COG:J", "GO:0005737", "GO:0006412", "GO:0006415", "GO:0008135", "GO:0043023", "SO:0001217", "UniRef:UniRef50_Q9PPX7", "UniRef:UniRef90_Q9PPX7" ], "nt": "ATGCACAATGAATTTATTAAATTGCGAACAGGAAGAGCTACGCCTGCTATTTTAGATGGTATTTTAGTTAATTATTATGGTTCAATGATGCCAATTAATCAATTAGCAAATATTTCAGTTCCAGAACCACGTGTTTTAGCAATTAAACCTTATGATAGAAGTAGTATTAAAGATATTGCTACTGCAATTAACGCATCAAATTTAGGTGTTAATCCTCAAGTAGATGTTGATATTATTCGTTTAACGTTCGCTGCTCCAACAGAAGAAGTAAGAAAAAATTTAGTTAAAAAAGCAAAACAGGTTGGCGAAGAAGCTAAAATTCGTGTACGTCATATCCGTCAAGAAGCACAAGATTTATTCAAAAAAGATTCAACAACTATTGAAGACGATAAAAAGTTTTTTCAAACAGAATTAGATAATTTAACAAAAGAATTAAATAAGGAAATCGAAACAGTTGTAAGTCATAAAGAAAAAGACATAATGACTGTTTAA", "aa": "MHNEFIKLRTGRATPAILDGILVNYYGSMMPINQLANISVPEPRVLAIKPYDRSSIKDIATAINASNLGVNPQVDVDIIRLTFAAPTEEVRKNLVKKAKQVGEEAKIRVRHIRQEAQDLFKKDSTTIEDDKKFFQTELDNLTKELNKEIETVVSHKEKDIMTV", "aa_hexdigest": "5e7fb3641b32aa1cbb6316fba742bf16", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_Q9PPX7", "query_cov": 1.0, "subject_cov": 0.8907103825136612, "identity": 1.0, "score": 307.0, "evalue": 3.37e-105, "valid": true, "gene": "frr", "product": "ribosome recycling factor", "uniref50_id": "UniRef50_Q9PPX7", "cog_id": "COG0233", "cog_category": "J", "go_ids": [ "GO:0005737", "GO:0006412", "GO:0006415", "GO:0008135", "GO:0043023" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PPX7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PPX7" ], "product": "Ribosome-recycling factor" }, "genes": [ "frr" ], "id": "NKIECMJPJD_533", "locus": "GCA000828735_00506" }, { "type": "cds", "sequence": "contig_1", "start": 288795, "stop": 288932, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAATATTAAAAACAATTGTTAAGATGATTAATGTTATCTTATTATTATCAAAAATGACTTTATTCATTTCATACGAACCAATACATATTAATGGTAATAATCATATTAATAAAACAATTGCAATCCCTTGTTGA", "aa": "MQILKTIVKMINVILLLSKMTLFISYEPIHINGNNHINKTIAIPC", "aa_hexdigest": "f1f0646574bb5c3101209075460887e6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5135.2691, "isoelectric_point": 9.516774559020995 }, "id": "NKIECMJPJD_534", "locus": "GCA000828735_00507" }, { "type": "cds", "sequence": "contig_1", "start": 289216, "stop": 289506, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATGATTGTTTTTTTAAGTGATACATTTGCATATGTTGGTGGTGTTTTATTTGGTAAAACAAAATTAGCACCAAAAATAAGTCCTAACAAAACAGTTGAAGGATTAATTTTTGGTTTAGTAATTGCAACAATTGTTATTATGTTAATAATTTTTGGTTTGTCATATGTGCCAATTAAACCTAAAAACGGCATAGTGTTAAAATCACAAAATGTTTTATATAATATTTTTGGTTTAGATTTTTCTAGTTCAAATGAAAAAAATCTTACATTGCATAAACAAGCATTATGA", "aa": "MMIVFLSDTFAYVGGVLFGKTKLAPKISPNKTVEGLIFGLVIATIVIMLIIFGLSYVPIKPKNGIVLKSQNVLYNIFGLDFSSSNEKNLTLHKQAL", "aa_hexdigest": "dafc4041d9a622f31fc8a551c4e55304", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10503.511099999989, "isoelectric_point": 9.699349021911619 }, "id": "NKIECMJPJD_535", "locus": "GCA000828735_00508" }, { "type": "cds", "sequence": "contig_1", "start": 289537, "stop": 289770, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAGTTTAATAAGCACTAGTGGGGACTTAGTATTTAGTGCGATTAAACGTAATTATCATACCAAAGATTATAGTAATTTAATTCCTGGCCATGGTGGATTATTAGATCGTATAGATTCACATTCATTTGTAATATCAATCATGTTCATTTTTACACTTCTTATTACAGGCAGTTTACAAGATGAAAATTTATTTCCTAACATTCAAAAATTTTTTATTCAATTAATTGTATAA", "aa": "MSLISTSGDLVFSAIKRNYHTKDYSNLIPGHGGLLDRIDSHSFVISIMFIFTLLITGSLQDENLFPNIQKFFIQLIV", "aa_hexdigest": "669fee5b4b6a45081dc7a0cb6f83a3c4", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8703.004199999994, "isoelectric_point": 6.222528266906737 }, "id": "NKIECMJPJD_536", "locus": "GCA000828735_00509" }, { "type": "cds", "sequence": "contig_1", "start": 289759, "stop": 290094, "strand": "-", "frame": 3, "gene": null, "product": "Transmembrane protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI000165F8A4", "UniRef:UniRef100_A0A2C9DZ10", "UniRef:UniRef50_A0AAC9T2J2", "UniRef:UniRef90_A0AAC9T2J2" ], "nt": "TTGGGTTTTTTATTATCTTTATTAATAGCAATTGGTGTAATGTTTGGATTTAAAAATAAAAAATATTTTAAAACGACATTAAACATTCAAACAAAATTATCTATAATCACTAAAAACAATTATGAAAAAATTAAAACCAAAAAACAACTAGAAATTAAAATTAAAAATTTAACTTATTATGTTAAATTTAAATTTTTAAAACAAACTCATGATTATTGCCTAATAAAAATCTTTCTTAGTTTAAAAACAGATCAAAAAATGATTGATATTAGTATATACACTAATACTCAACCATTAATTGAATCTATAATTAATAATTTCAAATTATACAATTAA", "aa": "MGFLLSLLIAIGVMFGFKNKKYFKTTLNIQTKLSIITKNNYEKIKTKKQLEIKIKNLTYYVKFKFLKQTHDYCLIKIFLSLKTDQKMIDISIYTNTQPLIESIINNFKLYN", "aa_hexdigest": "515044caf104f3d41f158096ebd06b60", "start_type": "TTG", "rbs_motif": "3Base/5BMM", "ups": { "uniparc_id": "UPI000165F8A4", "uniref100_id": "UniRef100_A0A2C9DZ10", "db_xrefs": [ "SO:0001217", "UniParc:UPI000165F8A4", "UniRef:UniRef100_A0A2C9DZ10" ] }, "ips": { "uniref100_id": "UniRef100_A0A2C9DZ10", "uniref90_id": "UniRef90_A0AAC9T2J2" }, "psc": { "uniref90_id": "UniRef90_A0AAC9T2J2", "product": "Transmembrane protein", "uniref50_id": "UniRef50_A0AAC9T2J2" }, "pscc": { "uniref50_id": "UniRef50_A0AAC9T2J2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T2J2" ], "product": "Transmembrane protein" }, "genes": [], "id": "NKIECMJPJD_537", "locus": "GCA000828735_00510" }, { "type": "cds", "sequence": "contig_1", "start": 290375, "stop": 290902, "strand": "-", "frame": 2, "gene": null, "product": "ATP-binding cassette domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00307F29B9", "UniRef:UniRef90_UPI00307F29B9" ], "nt": "TTGATTTTATTAACTTATCTAATCATTAATACCAATTATGATTATAAAGTTATTAGCGTAATCACTTATATAGTTTTTTTACATCAAAATCTAAATAATTTGTTTTTTGGTATCTTTAATTTTTTTAATGAATTATTAAATTTTAAAAATGGAAAACAAATTGTTGAAAAAATTTTGTATATTGATAATTATGAAGAACATGATGGTATACAAATTAATAAAATTACATCATGTAAAATTACCGAAAACCAAAAGGAATTTGAATTTAGCAAATCTTGTTTAATCATAGGAAAAAGTGGTGTTGGAAAAACAACATTATTAAAAAATTTTTTAAACTTTAATAATAAAAATTTATTTTTTAATAACTTAAATGTAAATAATATAAATTTAAATCAACTTAAAGAATTAATTATCTATCATCCAACAAATCCTTTAATAAATGATTTTGATATAAATTGGTTTATAGATAAAAATGAAGAAATTATTAATGCTTTAAAAATAGTTATTTTTATAACAAAGATCGATTGA", "aa": "MILLTYLIINTNYDYKVISVITYIVFLHQNLNNLFFGIFNFFNELLNFKNGKQIVEKILYIDNYEEHDGIQINKITSCKITENQKEFEFSKSCLIIGKSGVGKTTLLKNFLNFNNKNLFFNNLNVNNINLNQLKELIIYHPTNPLINDFDINWFIDKNEEIINALKIVIFITKID", "aa_hexdigest": "e33951c9a00d8a0cac1833f71bae1ed9", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 290099, "stop": 291202, "observations": { "mutation": [ 290377, 291136, 291199 ], "stop": [ 290377, 291136, 291199 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00307F29B9", "query_cov": 1.0, "subject_cov": 0.43859649122807015, "identity": 0.9940000000000001, "score": 306.0, "evalue": 1.47e-101, "gene_start": 1, "gene_end": 175, "reference_start": 133, "reference_end": 307, "reference_sequence": "MNNIIHFLLVSRIKLLVNTLFAICIICLLGLQNWSLLLISLGFSLLNFIIGLINWKYFKIHSLKIINIENSLFIKYQNFIKLINLEYNIDKYNNYINSYKKQFDELIIFKNKITIFKSNVNFYLSIINNIGLLILLTYLIINTNYDYKVISVITYIVFLHQNLNNLFFGIFNFFNELLNFKNGKQIVEKILYIDNYEEHDGIQINKITSCKITENQKEFEFSKSCLIIGKSGVGKTTLLKNFLNFNNKNLFFNNLNVNNINLNQLKELIIYHPTNPLINDFDINWFIDKNEEIINALKIVIFITKIDWSLNNNFEHNINLSTGQQQILAFLNLLKYKNKLLLLDEPLAHVDNDNKKIILNSILPIILKNNFVIYVSHDHYLKKYFDQIINLNDEKMDKY" }, "paralog": false, "identity": 0.9918256130790191, "score": 686.0, "evalue": 6.4e-254, "description": "Internal stop codon at 290377, 291136, 291199. Nonsense mutation around 290377, 291136, 291199.", "psc": { "uniref90_id": "UniRef90_UPI00307F29B9", "product": "ATP-binding cassette domain-containing protein", "uniref50_id": "UniRef50_UPI00307F29B9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00307F29B9", "UniRef:UniRef50_UPI00307F29B9" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00307F29B9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00307F29B9" ], "product": "ATP-binding cassette domain-containing protein" } }, "id": "NKIECMJPJD_538", "locus": "GCA000828735_00511" }, { "type": "cds", "sequence": "contig_1", "start": 291197, "stop": 292075, "strand": "-", "frame": 2, "gene": "sunT", "product": "ABC Transporter", "db_xrefs": [ "COG:COG2274", "COG:V", "SO:0001217", "UniRef:UniRef50_Q9PPY0", "UniRef:UniRef90_Q9PPY0" ], "nt": "ATGAAAATTGTTCAACAAACTTCTGATAATGAATGTGGTGTGTGTGTTATTAACATGTTAGCAAATTATTATCATAATAAAACAATTGATAAAAACATCATTCTTCAAAAAGCAAATTTAACAAAAAATGGTTTGAGTTTGCAAGAACTTGAAAACTTAGCAAATGAATTTAATTTAGATGCACAATCATTTGAATGTAGTTTTGAGGAGTTAATTGATGAAAAAATCAATGACTATTTTATAGCTTTAATTAACAAAAACGGTTTGAATCATTTTGTTATTGTTAAAAATTATAACAAAAATTTTTTTCGTATTTATGATCCAGAAAATAAAATATATGAATTAAATAATGAAGAATTTAAAAAAATATTTTTAAATATAATTGTGCGTGTTTCTAAAAATCATCAAATTTTTGACCTTCCCATATTTAAAGAATCTTATTTAAAAAATATTAAACTCTCATCGTTAATTATCATATTATTTATAGAGTTATTAAATATTCCTTTAAATATTTTTTTAAGTAAAATTGTAAACTTACTAATAGATTTAGTTTTAATTGACACACAAATTAAAAACTTAACATACTTATTTATTGTTTTTAGTTTATTTTATATTAGTAATGCTTTCAAAGATTTACTAATTACTATATACATAAATAAAATTAGTAATAATATTTTTTCTAATATTAATAATAAAATAGTTAATAATTTAGCTAATAAATCAATTATTTTTTTTAATAAAACAAATTTAGGTGAATTAAGCTCGATTCAGATTCATTTGAATAATATAATTCATTTTCTATTAGTTAGTCGTATAAAATTATTAGTTAATACTTTATTTGCAATTTGTATTATTTGTTTATTAGGTTTACAAAATTGA", "aa": "MKIVQQTSDNECGVCVINMLANYYHNKTIDKNIILQKANLTKNGLSLQELENLANEFNLDAQSFECSFEELIDEKINDYFIALINKNGLNHFVIVKNYNKNFFRIYDPENKIYELNNEEFKKIFLNIIVRVSKNHQIFDLPIFKESYLKNIKLSSLIIILFIELLNIPLNIFLSKIVNLLIDLVLIDTQIKNLTYLFIVFSLFYISNAFKDLLITIYINKISNNIFSNINNKIVNNLANKSIIFFNKTNLGELSSIQIHLNNIIHFLLVSRIKLLVNTLFAICIICLLGLQN", "aa_hexdigest": "904fe3950a25a1156ef07109a1dbb466", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "genes": [ "sunT" ], "truncated": "5-prime", "pseudogene": { "start": 290894, "stop": 292075, "observations": { "mutation": [ 291136, 291199 ], "stop": [ 291136, 291199 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PPY0", "query_cov": 1.0, "subject_cov": 0.44376899696048633, "identity": 1.0, "score": 530.0, "evalue": 8.41e-184, "gene_start": 1, "gene_end": 292, "reference_start": 1, "reference_end": 292, "reference_sequence": "MKIVQQTSDNECGVCVINMLANYYHNKTIDKNIILQKANLTKNGLSLQELENLANEFNLDAQSFECSFEELIDEKINDYFIALINKNGLNHFVIVKNYNKNFFRIYDPENKIYELNNEEFKKIFLNIIVRVSKNHQIFDLPIFKESYLKNIKLSSLIIILFIELLNIPLNIFLSKIVNLLIDLVLIDTQIKNLTYLFIVFSLFYISNAFKDLLITIYINKISNNIFSNINNKIVNNLANKSIIFFNKTNLGELSSIQIHLNNIIHFLLVSRIKLLVNTLFAICIICLLGLQNWSLLLISLGFSLLNFIIGLINWKYFKIHSLKIINIENSLFIKYQNFIKLINLEYNIDKYNNYINSYKKQFDELIIFKNKITIFKSNVNFYLSIINNIGLLILLTYLIINTNYDYKVISVITYIVFLHQNLNNLFFGIFNFFNELLNFKNGKQIVEKILYIDNYEEHDGIQINKITSCKITENQKEFEFSKSCLIIGKSGVGKTTLLKNFLNFNNKNLFFNNLNVNNINLNQLKELIIYHPTNPLINDFDINWFIDKNEEIINALKIVIFITKIDWSLNNNFEHNINLSTGQQQILAFLNLLKYKNKLLLLDEPLAHVDNDNKKIILNSILPIILKNNFVIYVSHDHYLKKYFDQIINLNDEKMDKY" }, "paralog": false, "identity": 0.9949109414758269, "score": 748.0, "evalue": 3.69e-272, "description": "Internal stop codon at 291136, 291199. Nonsense mutation around 291136, 291199.", "psc": { "uniref90_id": "UniRef90_Q9PPY0", "gene": "SunT", "product": "ABC Transporter", "uniref50_id": "UniRef50_Q9PPY0", "cog_id": "COG2274", "cog_category": "V", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PPY0", "UniRef:UniRef50_Q9PPY0", "COG:COG2274", "COG:V" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PPY0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PPY0" ], "product": "ABC Transporter" } }, "id": "NKIECMJPJD_539", "locus": "GCA000828735_00512" }, { "type": "cds", "sequence": "contig_1", "start": 292215, "stop": 293423, "strand": "+", "frame": 3, "gene": "rnjA", "product": "Ribonuclease J C-terminal domain-containing protein", "db_xrefs": [ "COG:COG0595", "COG:J", "SO:0001217", "UniRef:UniRef50_A0A2C9DYT9", "UniRef:UniRef90_A0A2C9DYT9" ], "nt": "ATGGCTAAAATTAATTTTTTATCGTTAGGAGGACAAGATGAAAGAGGAAAATCTTGCTTTGTTCTTGAAGTGAACGATGATATTTTTATTTTTAATGCTGGTGCGAAAATTCCAACAAGTGATGTTTTTGGCGTAAATATGATTGTTTGTGATTATTCTTATTTAGAAAAAAACGCAAAACGCGTTAAGGGAATTTTTATTGGTACACCAACATTTAATAATGTTATGGGAATTAAATTATTACTAGGACAAGTAGGTTATAAAATCCCAATTTACACAAGCCCAATAGGAGCAATTGTTATTAAAAAAATTTTTGAACAAAAAGTAAACAATAAAAAAATTGAACCTAATATTATTGAGCTAGATCCAATTTCAGATAAGAAAATTGGATCAATTTATGTTACATCTTTTAAAGTTTCAAACTCAATGCCTCATTCATATGGATTTGTATTAAAAACAAGCGATGGAGCAATTGTTTATGTTGATGAGTTTATTATTTCAAATGATAAAAATAAAACCTTTGATTCACAAATTAATCTTTTAAATAACATTACTAAAAATAATACTCTAGCTTTGATTGTAGGAATGGGTCAAGCAGGAAATCCTTATTTTACTGCTCCCAATCATAAAAATAAGGCTTTTTATGAAGCTGCATTGCAAAATACTAAAAATCGTTTAATTGTTGGTTGTTATAGCAATGATGCATATAGTATTTTTACTTTGGCAACAATTGCAAAACAACAAAATCGCCCCTTTATTGTTTATAGTAATAACTTCATTAACACTTTTATTGGTGTATTGAAATTGAAATTATTTAATAGTAAAAATTTAATTTCTTTACCGGTTTCTGAAATTAATAATTCAAAAAATGCAATTATTGTGGTAATTGAAAATCAAGATACTCTTTTTTCAAAATTAAATAAAATTCTACATAACGAAGATAAATATGTTAATTTGTCTTCTGATGATCAACTAATATTAGGCGTTGTTATTACACCAGGTTTTGAAATGTTAGCAGCTGAACTAAGTGATGAAGTAGGTCGTTTAGACATTCCTTATAAAGCATTACCTAAAACAGTTTTACCAATGACACAATCTGATGAAGATTTAAAACATCTAATTAATTTTTTACAACCAAAATTTTTAATTCCAATTAATGGATTATATAAAACTGAGGTTAAATTTAGTTCTACAGTTACAACATCTTGA", "aa": "MAKINFLSLGGQDERGKSCFVLEVNDDIFIFNAGAKIPTSDVFGVNMIVCDYSYLEKNAKRVKGIFIGTPTFNNVMGIKLLLGQVGYKIPIYTSPIGAIVIKKIFEQKVNNKKIEPNIIELDPISDKKIGSIYVTSFKVSNSMPHSYGFVLKTSDGAIVYVDEFIISNDKNKTFDSQINLLNNITKNNTLALIVGMGQAGNPYFTAPNHKNKAFYEAALQNTKNRLIVGCYSNDAYSIFTLATIAKQQNRPFIVYSNNFINTFIGVLKLKLFNSKNLISLPVSEINNSKNAIIVVIENQDTLFSKLNKILHNEDKYVNLSSDDQLILGVVITPGFEMLAAELSDEVGRLDIPYKALPKTVLPMTQSDEDLKHLINFLQPKFLIPINGLYKTEVKFSSTVTTS", "aa_hexdigest": "f16099ec92d1fea85d01cca8bc071e34", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [ "rnjA" ], "truncated": "3-prime", "pseudogene": { "start": 292215, "stop": 293726, "observations": { "mutation": [ 293421 ], "stop": [ 293421 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A2C9DYT9", "query_cov": 1.0, "subject_cov": 0.7178571428571429, "identity": 0.948, "score": 758.0, "evalue": 1.88e-273, "gene_start": 1, "gene_end": 402, "reference_start": 1, "reference_end": 402, "reference_sequence": "MAKINFLSLGGQDERGKSCFVLEVNDDIFIFNAGAKIPTSDVFGVNMIVCDYSYLEKNAKRVKGIFIGTPTFNNVMGIKLLLAQVGYKIPIYTSPIGAIVVKKIFEQKVNNKKIEPNIIELDPISDKKIGSIYVTAFKVSNSMPHSYGFVLKTSDGAIVYIDEFIISNDKNKTFDSQINALNNITKNNTLALIVGMGQAGNPCFTAPNHKNKGFYESILQNTKNRLIVGCYSNDAYSIFTLATIAKQQNRPFIVYSNNFINTFVGVLKLKLFNSKNLISLPVSEINNSKNAIIVVIENQDTLFPRLNKILHNEDKHITLTSEDQLVLGVVITPGFEMLAAQLSDEVGRLDIPYKALPKTVLPMTQSDEDLKHLINFLQPKYLIPINGLYKTEVKFTSTVTTSWIKSDQIISISNGELFSIEDKVLNPKPQVIELEDKYISSFDALDVGANILFERAQMGENGVINLIVIFDKQFQKLFNYVEFDYCGVVNDDSQVRAQVKEIEETFKKRMGECLVYDDRKRLVLKDTKASLKRLLTKLFEKKFNKRPLVLPTVVDCYKTK" }, "paralog": false, "identity": 0.9368836291913215, "score": 945.0, "evalue": 0.0, "description": "Internal stop codon at 293421. Nonsense mutation around 293421.", "psc": { "uniref90_id": "UniRef90_A0A2C9DYT9", "gene": "RnjA", "product": "Ribonuclease J C-terminal domain-containing protein", "uniref50_id": "UniRef50_A0A2C9DYT9", "cog_id": "COG0595", "cog_category": "J", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A2C9DYT9", "UniRef:UniRef50_A0A2C9DYT9", "COG:COG0595", "COG:J" ] }, "pscc": { "uniref50_id": "UniRef50_A0A2C9DYT9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2C9DYT9" ], "product": "Ribonuclease J C-terminal domain-containing protein" } }, "id": "NKIECMJPJD_540", "locus": "GCA000828735_00513" }, { "type": "cds", "sequence": "contig_1", "start": 293586, "stop": 293885, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF17770.6" ], "nt": "ATGGGCGAGAATGGTGTTATTAATTTAATTGTTATTTTTGATAAACAATTCCAAAAACTATTTAATTATGTTGAATTTGATTATTGTGGAGTTGTTAATAATGACGCTCAAATTAAAGAAATTGAAGAAACATTTAAAAAACGTATGGGTGAATGTTTAGTTTATGATGAACGTAAACGCTTAATACTAAAAGATACCAAAGCAAATTTAAAACGTTTATTAACTAAATTATTTGAAAAGAAATTTAATAAGCGTCCATTAGTTTTGCCAACAGTAGTTGACTGTTATAAAACAAAATAA", "aa": "MGENGVINLIVIFDKQFQKLFNYVEFDYCGVVNNDAQIKEIEETFKKRMGECLVYDERKRLILKDTKANLKRLLTKLFEKKFNKRPLVLPTVVDCYKTK", "aa_hexdigest": "a4dc15b57a17e34d1f3bb87ed91bdadd", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF17770.6", "name": "RNase_J_C", "length": 95, "aa_cov": 0.9494949494949495, "hmm_cov": 0.9894736842105263, "evalue": 8.133760496507858e-10, "score": 35.219783782958984, "start": 1, "stop": 94 } ], "seq_stats": { "molecular_weight": 11768.76969999998, "isoelectric_point": 9.278306007385254 }, "id": "NKIECMJPJD_541", "locus": "GCA000828735_00514" }, { "type": "cds", "sequence": "contig_1", "start": 293902, "stop": 294186, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E4F2E94", "UniRef:UniRef90_UPI001E4F2E94" ], "nt": "ATGGTTAGTGACAATAAAAACACATTAAATAAGGAAACAATTAGTTTTTATAGTTATGACAATCAACTAGATGCACAAAAAACAGAATCAAACGGAAAAGAACAAGAAAAAAGCAATTTAAAGACAAAAAAATTCAAAGTTCAAAAAAATTATTTTTTTAATAAAAAAAAGCTTATTGGAATTTGTTTTTTTATATTAAGCTTTTTAATTTTAATACTTGCTTTTTTAAAAGCCCCATATACAGGAGCGATTTTAGACAATGTTTTAGAATTTTTTTTTGGATGA", "aa": "MVSDNKNTLNKETISFYSYDNQLDAQKTESNGKEQEKSNLKTKKFKVQKNYFFNKKKLIGICFFILSFLILILAFLKAPYTGAILDNVLEFFFG", "aa_hexdigest": "8236c1a2ddbe1d88fc14d414e4f0e993", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_UPI001E4F2E94", "query_cov": 0.9893617021276596, "subject_cov": 0.9117647058823529, "identity": 1.0, "score": 174.0, "evalue": 5.41e-55, "valid": true, "uniref50_id": "UniRef50_UPI001E4F2E94" }, "pscc": { "uniref50_id": "UniRef50_UPI001E4F2E94", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E4F2E94" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10935.575899999989, "isoelectric_point": 9.353089332580566 }, "id": "NKIECMJPJD_542", "locus": "GCA000828735_00515" }, { "type": "cds", "sequence": "contig_1", "start": 294274, "stop": 294489, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTGTTTTTTATATTATTACACTAATTTTATTTGCTTTAATTATTGGTGCAGTTGGTTATGGAATTATCACAAATTCATTAACTTATAAATTCATTATTAATACAAATCAACAAATGATTAATAAGGAAATACTTAATTTACATAAAGATAATTTCGAATATATTAGTTTAGTAAATTTACACTCAAAAAAATTACATTTATGTGAGTGGTGA", "aa": "MIVFYIITLILFALIIGAVGYGIITNSLTYKFIINTNQQMINKEILNLHKDNFEYISLVNLHSKKLHLCEW", "aa_hexdigest": "154561600ba5e9597d96d9755216c956", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8299.808499999997, "isoelectric_point": 7.906612205505372 }, "id": "NKIECMJPJD_543", "locus": "GCA000828735_00516" }, { "type": "cds", "sequence": "contig_1", "start": 294835, "stop": 295431, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAGCAAAAAGAAGTTATCCAAGAACAAAATTTACAAATTAAAAATTTTAATGATCGGCAAACTAATTCTTCATGTATCACAAAAAATGATAAACTAAAAACCACTATTTTAGAACCAATTGTTAATGATATTAACCAATTCCGAGAAAATCCATCAACAATCAAGAATAACAAGTACAATTTTTATCCACGAATTGATATTATAGATGGCAAAAGTCAAGATTATTTGCATGAACTTAAATCAAAAGGTGAAAATTTAAAATTAATTATCGATAAATTTTTAGAAAATAACCAATTAGAATATCATTTTAATGCAATTAATCCTTATTTTTCAAATGTTGAAATTGTTTATCAGTTTACTCGTTCATCATTAAACAATTTTTTAAAAAATTTTTTAAATTTAATGAAACAAGCAGTTAATGATACAGATGATTATGAAATCAATATTTATAATGACAATGACTTTTTAATCATTCAAGCCAAAGTCAAAGATTTAAATCCTCAAATTAATATTAAAGATATTCTAACTAATTTAGAATACAATGATAAATTATGTTTAGGAATTGGAAAATTAAAAGAAAGAAAAGTAGTTTGA", "aa": "MEQKEVIQEQNLQIKNFNDRQTNSSCITKNDKLKTTILEPIVNDINQFRENPSTIKNNKYNFYPRIDIIDGKSQDYLHELKSKGENLKLIIDKFLENNQLEYHFNAINPYFSNVEIVYQFTRSSLNNFLKNFLNLMKQAVNDTDDYEINIYNDNDFLIIQAKVKDLNPQINIKDILTNLEYNDKLCLGIGKLKERKVV", "aa_hexdigest": "da247066df931da7b5178d7500c20e6f", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 23403.26580000003, "isoelectric_point": 5.788789939880371 }, "id": "NKIECMJPJD_544", "locus": "GCA000828735_00517" }, { "type": "cds", "sequence": "contig_1", "start": 295495, "stop": 296004, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01580.24" ], "nt": "ATGTTAATTTCTAACATTATTATTAGTGCTTTATATACTAAATCACCTAATGAATTAGAATTATTTATTATTAATAATGGTTCAAAATCATTAAAAGAATTTGCTAAATTAAAGCATACAAAAAAAAGCGTTGATCATGATGATTTTGAAAATGTTATTAATTTATTAAGGGAAATTATGAATGATATTAACAAGCAAAATACATTATTTGCTGATAATAATGTTGATAATTTAGATGAATATAATCTAAAAAATCAAAATCAAAAATTACCCAAAAAATTAATCATTATTTCTGAATATGTTGAGATAGTAAGTAGTCAATTTAATACACGTTTTGATACCTTAATTCGCAATATAGCAAATATTGCTAAAAAACACGGAATCGTTTTAATAATTTCATCAAATATAACTAACGAAAGCACAGTTTCTTTTAAAAATGTATTTGATTATACCATTGTATTAAAATTAAATAATCCGTATGAAAGTATTTTATTAACTGATCGTAATTGA", "aa": "MLISNIIISALYTKSPNELELFIINNGSKSLKEFAKLKHTKKSVDHDDFENVINLLREIMNDINKQNTLFADNNVDNLDEYNLKNQNQKLPKKLIIISEYVEIVSSQFNTRFDTLIRNIANIAKKHGIVLIISSNITNESTVSFKNVFDYTIVLKLNNPYESILLTDRN", "aa_hexdigest": "716bf21fb02d96fe61cc041a66699506", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01580.24", "name": "FtsK_SpoIIIE", "length": 163, "aa_cov": 0.9408284023668639, "hmm_cov": 0.8220858895705522, "evalue": 1.976225925631386e-12, "score": 42.594505310058594, "start": 3, "stop": 161 } ], "seq_stats": { "molecular_weight": 19475.070000000007, "isoelectric_point": 6.838435554504395 }, "id": "NKIECMJPJD_545", "locus": "GCA000828735_00518" }, { "type": "cds", "sequence": "contig_1", "start": 296032, "stop": 296205, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTATTAATTCGCAATTTTGATAATTTGCCACTACGATTGCAAACAGCAAAAATTACAAATGAACAGTTTGCTAATATTATTAATGAAATCAATAGTGCTGATTATGATATTGATGAATATCGTCATGAAACAAGATTAACATCAAGAACCCAAAATTTTTTTAATAAATAA", "aa": "MLLIRNFDNLPLRLQTAKITNEQFANIINEINSADYDIDEYRHETRLTSRTQNFFNK", "aa_hexdigest": "72b085683ba5c6c51732b7c19ddf70cb", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6860.593500000001, "isoelectric_point": 5.636461448669433 }, "id": "NKIECMJPJD_546", "locus": "GCA000828735_00519" }, { "type": "cds", "sequence": "contig_1", "start": 296318, "stop": 297055, "strand": "+", "frame": 2, "gene": "pepB", "product": "leucyl aminopeptidase", "db_xrefs": [ "COG:COG0260", "COG:E", "EC:3.4.11.1", "SO:0001217", "UniRef:UniRef50_A0AAP9ACA2", "UniRef:UniRef90_A0AAP9ACA2" ], "nt": "ATGACATTAAATGAAAAAAAAGAATTTGTTTATGAATTAAAAGCTGTTGAAAAAAATCACAACCTAAAAGGTTTTGAAGAAAATACAACTGAACATGTTGTTTTTGGAGGTTTATATCAAAAACAACAATACTTAATTTTAACACCAGATTTTGATTCTAATGAACTATCAAGATCATTGATTAGTTTTTTAGAAAAATCACCTAAATCTGTTAGTGTTGATTTAAATTCATTTCTATCACTTGTCCCAGAATCAAGACATGCATCTTTATTAAATGTTGTTATTAGTGCATTAGAATATGTTGAAGTAACACCATTTTCACTAAAATCTAAAATTGAAACAAAAAATATACATAATTTAGTAGTTGATCAAAAATATCATGATTTAATTAATAAATTACATGTAATTGCTCAATCACAAACAATTACAAGAACATTACAAGATACGCCAGCTAATTTAATGACACCAGGTGATTTTGAAGAACGAATTAAAGAATTATTTAAAGACTTGCCAGAAGTTAAAGTAAGTGTTTTATATCGTAAAGATTTAGAAGCTAAAGGAATGAACGCTCATGTTGGTGTTGGTAAAGCTGCAGTAAGTGATAAAGCACAGCCTCGTTTAGTTGTTGTTGAATATAATAATAATCCAGAAACAAATGAAAAATATGCTTTTGTTGGTAAGGGTGTATGTTTTGATTCAGGTGGATATAATGTAAAAACGGGATCACATATGCGTTGA", "aa": "MTLNEKKEFVYELKAVEKNHNLKGFEENTTEHVVFGGLYQKQQYLILTPDFDSNELSRSLISFLEKSPKSVSVDLNSFLSLVPESRHASLLNVVISALEYVEVTPFSLKSKIETKNIHNLVVDQKYHDLINKLHVIAQSQTITRTLQDTPANLMTPGDFEERIKELFKDLPEVKVSVLYRKDLEAKGMNAHVGVGKAAVSDKAQPRLVVVEYNNNPETNEKYAFVGKGVCFDSGGYNVKTGSHMR", "aa_hexdigest": "88ef1d847b061d2a66aed0354a6aaf2b", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "genes": [ "pepB" ], "truncated": "3-prime", "pseudogene": { "start": 296318, "stop": 297358, "observations": { "mutation": [ 297053 ], "stop": [ 297053 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAP9ACA2", "query_cov": 1.0, "subject_cov": 0.5384615384615384, "identity": 0.927, "score": 449.0, "evalue": 7.84e-156, "gene_start": 1, "gene_end": 245, "reference_start": 1, "reference_end": 245, "reference_sequence": "MTLNEKKEFVYELKAVKKDHNLKGFEKNTAEHVVFGEVYQKQHYLILTPDFDSNELSRSLVSFLEKSPKPVSVDLNSFLDLVPESRHASLLNVVVSALEYVEVTPFSLKSKVEPKNIHNLVVDAKYHDLIAKLQVIAQSQTITRTLQDTPANLMTPGDFEERIKELFKDLPEVKVSVLYRKDLEAKGMNAHVGVGKAAVSDKAQPRLVVVEYNNNPDTDEKYAFVGKGVCFDSGGYNVKTGSHMRWMKFDMSGSAIVSMTVRALALNKEKVNVVAVCPLVLNLLAPEGQKPDDIIKSYNGKTIELDNTDAEGRLILADALTYAVRDLKASKLFDIATLTGAMIFALGDTYSGVWATNNDIWNEVVVAADYAGELVWRLPFHNDFLKMLNSNVADIANSVTDPRGGSSRAACFLKEFTEGVPYAHFDIAITADVGHKGTGVMLRTFYRIAQNQKFN" }, "paralog": false, "identity": 0.9364161849710982, "score": 638.0, "evalue": 8.52e-233, "description": "Internal stop codon at 297053. Nonsense mutation around 297053.", "psc": { "uniref90_id": "UniRef90_A0AAP9ACA2", "gene": "PepB", "product": "leucyl aminopeptidase", "ec_ids": [ "3.4.11.1" ], "uniref50_id": "UniRef50_A0AAP9ACA2", "cog_id": "COG0260", "cog_category": "E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAP9ACA2", "EC:3.4.11.1", "UniRef:UniRef50_A0AAP9ACA2", "COG:COG0260", "COG:E" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAP9ACA2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAP9ACA2" ], "product": "Leucyl aminopeptidase" } }, "id": "NKIECMJPJD_547", "locus": "GCA000828735_00520" }, { "type": "cds", "sequence": "contig_1", "start": 297056, "stop": 297379, "strand": "+", "frame": 2, "gene": null, "product": "Cytosol aminopeptidase domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A5A7N3P8" ], "nt": "ATGAAATTTGATATGTCTGGTTCTGCTATTGTAAGTATGACAGTGAGAGCTTTAGCTTTAAATAAAGAAAAAGTTAATGTTGTGGCTGTATGTCCATTAGTATTAAATTTATTAGCTCCAGAAGGTCAAAAACCAGATGATATAGTGAAATCATATAATGGTAAAACAATTGAATTAGATAACACTGATGCAGAAGGACGTTTAATTTTAGCAGATGCACTAACATATGCTGTTCGTGATTTAAAAGCTTCTAAATTATTTGATGTTGCAACTTTAACAGGTGCTATGATTTATGCTTTAGGTGATACATATTCTGGTGTATGA", "aa": "MKFDMSGSAIVSMTVRALALNKEKVNVVAVCPLVLNLLAPEGQKPDDIVKSYNGKTIELDNTDAEGRLILADALTYAVRDLKASKLFDVATLTGAMIYALGDTYSGV", "aa_hexdigest": "3c31fce772513910ea5efcc358a72d89", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "psc": { "uniref90_id": "UniRef90_A0A5A7N3P8", "query_cov": 0.8504672897196262, "subject_cov": 0.8348623853211009, "identity": 0.516, "score": 85.5, "evalue": 1.61e-19, "valid": false, "product": "Cytosol aminopeptidase domain-containing protein", "uniref50_id": "UniRef50_A0A5A7N3P8" }, "pscc": { "uniref50_id": "UniRef50_A0A5A7N3P8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A5A7N3P8" ], "product": "Cytosol aminopeptidase domain-containing protein" }, "genes": [], "id": "NKIECMJPJD_548", "locus": "GCA000828735_00521" }, { "type": "cds", "sequence": "contig_1", "start": 297476, "stop": 297685, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00883.26" ], "nt": "ATGTTAAATTCAAATGTTGCTGATATTGCTAACTCAGTATCTGATCCTCGTGGTGGTTCTTCACGTGCAGCATGTTTCTTAAAAGAATTTACTGAAGGCGTTCCATATGCTCATTTTGACATTGCTATTACTGCAGATGTTGGTCATAAAGGAACTGGTGTAATGTTAAGAACATTTTATCGTATTGCTCAAAATCAAAAATTTAATTAG", "aa": "MLNSNVADIANSVSDPRGGSSRAACFLKEFTEGVPYAHFDIAITADVGHKGTGVMLRTFYRIAQNQKFN", "aa_hexdigest": "33932f43f0753e41d32c6bc2e60dcd24", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00883.26", "name": "Peptidase_M17", "length": 66, "aa_cov": 0.8405797101449275, "hmm_cov": 0.9848484848484849, "evalue": 3.447944710586185e-13, "score": 45.05320358276367, "start": 2, "stop": 59 } ], "seq_stats": { "molecular_weight": 7507.374599999999, "isoelectric_point": 7.944197273254395 }, "id": "NKIECMJPJD_549", "locus": "GCA000828735_00522" }, { "type": "tRNA", "sequence": "contig_1", "start": 298653, "stop": 298727, "strand": "-", "gene": null, "product": "tRNA-Xxx", "score": 73.7, "nt": "AGGGGTATAGTTCAATGGTAGAACTGCGGACTTCAAATCCGCGTGTTGTGGGTTCGAGTCCTGCTACCCCTGCCA", "db_xrefs": [], "id": "NKIECMJPJD_29", "locus": "GCA000828735_00523" }, { "type": "cds", "sequence": "contig_1", "start": 298791, "stop": 299024, "strand": "-", "frame": 1, "gene": "acpP", "product": "Acyl carrier -like protein", "db_xrefs": [ "COG:COG0236", "COG:I", "GO:0006633", "RefSeq:WP_006688580.1", "SO:0001217", "UniParc:UPI000012531D", "UniRef:UniRef100_Q9PPY4", "UniRef:UniRef50_Q9PPY4", "UniRef:UniRef90_Q9PPY4" ], "nt": "ATGTCTATTAATATTAAAGATTTAATCATGAAAATCGCTAAGGAAAATAAAATTGCTTTAAATATGGATAATTTAAATATCGAATTAAAATCATTAGGAATTGATTCGCTAAGTGCTATGAATTTAATAATGAAAATTGAGGATCAAATTGGCGTTCAATTGCCTGATGAAAAATTATTAAAAATCAAAAATTTACGTGATTTAATTAACGCGTTTGAAGATGTATTAAAATAA", "aa": "MSINIKDLIMKIAKENKIALNMDNLNIELKSLGIDSLSAMNLIMKIEDQIGVQLPDEKLLKIKNLRDLINAFEDVLK", "aa_hexdigest": "3679fe4270a1d663e5c2b0ff64e2bb56", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000012531D", "ncbi_nrp_id": "WP_006688580.1", "uniref100_id": "UniRef100_Q9PPY4", "db_xrefs": [ "SO:0001217", "UniParc:UPI000012531D", "RefSeq:WP_006688580.1", "UniRef:UniRef100_Q9PPY4" ] }, "ips": { "uniref100_id": "UniRef100_Q9PPY4", "uniref90_id": "UniRef90_Q9PPY4" }, "psc": { "uniref90_id": "UniRef90_Q9PPY4", "gene": "AcpP", "product": "Acyl carrier protein homolog", "uniref50_id": "UniRef50_Q9PPY4", "cog_id": "COG0236", "cog_category": "I", "go_ids": [ "GO:0006633" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PPY4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PPY4" ], "product": "Acyl carrier protein homolog" }, "genes": [ "acpP" ], "id": "NKIECMJPJD_550", "locus": "GCA000828735_00524" }, { "type": "cds", "sequence": "contig_1", "start": 299110, "stop": 299901, "strand": "-", "frame": 3, "gene": null, "product": "Lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2C9DY82", "UniRef:UniRef90_A0A2C9DY82" ], "nt": "ATGAAAATTTTAAATCACTTGAAAATTAATTGGCGTAGTCATAAAATAAAGGTGATTGTTGGTGGAATTATTAGTTTATTAATTGTAATTACTGCTATTATTTTAGGTGTTATTCTATCAACTCAAAATACAAATAAAATTAGTAAAAAAGAACCCTTAACTCAAATTGAAGATAATATTAATAATAAAAAACAAGATGCACATAAGGTTAATGAAAATATAACAATTATTGATAAAAAAGGTGGTATAATCCATAAAAAGACAGACATTACAAAAATTAACCAAATAAATCAACAAAAAGAAATTTTAAAGGATTATTTAAAAAATAAAGAAGAAAAAAATAAGGATCAAATAAATAATACTAACGAAAATCTAAATAAACCAATAATTAATGTTAAAAATGTAGATGATAAAAACAAACAAGAAAATTCAACTAAATTAAAAAATAATGATTTTATTTCTAATAATGATAAAAACAATAAAATCAACGAAAATAATAATATAAGTTATGAAGAAAAACCTTTTAAACTAAAACGCTTCAATATTATTAATGTAATTGCGAAAAATAAAGATTTATACCAATTATTAGATTTCAAAACTTATTTTTCTAATGAAAACATTAATAATGATATTAAATTTAATGAAAAGTTGTTTATTAAAAATATCTATGAAATAGTCAAATCTGCTATAAGTAGTTTTCAAGAATTTTGTAATATCATGCAGTATATTAAAATTGATATCAAATATAAATTTAATAAAGATGCAAAAAACATAATTGTTATTGCTAATTGA", "aa": "MKILNHLKINWRSHKIKVIVGGIISLLIVITAIILGVILSTQNTNKISKKEPLTQIEDNINNKKQDAHKVNENITIIDKKGGIIHKKTDITKINQINQQKEILKDYLKNKEEKNKDQINNTNENLNKPIINVKNVDDKNKQENSTKLKNNDFISNNDKNNKINENNNISYEEKPFKLKRFNIINVIAKNKDLYQLLDFKTYFSNENINNDIKFNEKLFIKNIYEIVKSAISSFQEFCNIMQYIKIDIKYKFNKDAKNIIVIAN", "aa_hexdigest": "7ca3f3936526376426828e90952f11d4", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A2C9DY82", "query_cov": 1.0, "subject_cov": 0.9195804195804196, "identity": 1.0, "score": 444.0, "evalue": 2.93e-156, "valid": true, "product": "Lipoprotein", "uniref50_id": "UniRef50_A0A2C9DY82" }, "pscc": { "uniref50_id": "UniRef50_A0A2C9DY82", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2C9DY82" ], "product": "Lipoprotein" }, "genes": [], "id": "NKIECMJPJD_551", "locus": "GCA000828735_00525" }, { "type": "cds", "sequence": "contig_1", "start": 300413, "stop": 301015, "strand": "-", "frame": 2, "gene": null, "product": "DUF1410 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T0I9", "UniRef:UniRef90_A0AAC9T0I9" ], "nt": "ATGATCAATAATTCACCAAACATTAATTATTATTTACAAGATAAAAAATATATTCCAAAATATGCTTACTATGTTAATAAAAAACAAAAACCTATCGAGCTATTTGCACGATATAAAGGACGTCCTGCTCTTTTTATTCGTGAAAATACCATGATTAATACTTCATTAAATTCAAATGTATTAAATTTAGACTTTGAATTTGAAGTTGCAGGAGATTTTAAATTACCTATTAATTACTTTTTTATGGATATAAAAAGTGATATAGGTGAATTTCGTAATTTAGAAGTTTATGAAACTAAACACTCTTTGTATGAAATCGGAGGAGTGATTTATGACTTATACACTTATAAAACCAATTTAAAAATTAATCTTAATCAAGCTGGTTTTGTTAATAAGAACACTTTAAGTAAAAAAATTAATTTTGCTTTTTTTCTTCGTATTAATGATGATAAACGAATTCATGAACAAATTAATTATACATGTGAAAAAAGTTTGTTTTTTGAGGCAGTACAAAAAGAATTTCAAGATCTTAATTTTGATTATTTTTACAAAAATACATTTTTATTAAATAAACACGAAAAAGTTAATCCTACTAATTTTTGA", "aa": "MINNSPNINYYLQDKKYIPKYAYYVNKKQKPIELFARYKGRPALFIRENTMINTSLNSNVLNLDFEFEVAGDFKLPINYFFMDIKSDIGEFRNLEVYETKHSLYEIGGVIYDLYTYKTNLKINLNQAGFVNKNTLSKKINFAFFLRINDDKRIHEQINYTCEKSLFFEAVQKEFQDLNFDYFYKNTFLLNKHEKVNPTNF", "aa_hexdigest": "b498cecc261f1848b2caad9cb80f8518", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 300110, "stop": 301036, "observations": { "mutation": [ 300208, 300298, 300394, 300415 ], "stop": [ 300208, 300298, 300394, 300415 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAC9T0I9", "query_cov": 1.0, "subject_cov": 0.5167958656330749, "identity": 1.0, "score": 403.0, "evalue": 1.53e-139, "gene_start": 1, "gene_end": 200, "reference_start": 8, "reference_end": 207, "reference_sequence": "MKKKVIKMINNSPNINYYLQDKKYIPKYAYYVNKKQKPIELFARYKGRPALFIRENTMINTSLNSNVLNLDFEFEVAGDFKLPINYFFMDIKSDIGEFRNLEVYETKHSLYEIGGVIYDLYTYKTNLKINLNQAGFVNKNTLSKKINFAFFLRINDDKRIHEQINYTCEKSLFFEAVQKEFQDLNFDYFYKNTFLLNKHEKVNPTNFWETIPLFWIQDKELDNEIKIKNNKRYIFNTKFSLNDDNSWNNRPTKETFELIKYSLEYVNLAKENKKIEWIEDYQNINITYDYYQRPIDFLLKMKTKFLYDFKNDQLSINNNYGFNGIWLPNKQIAKLKITVINNYLTPQKFVINQLINIENDFNSINKQKISVQKAIIENYENFKSLEN" }, "paralog": false, "identity": 0.987012987012987, "score": 615.0, "evalue": 1.65e-225, "description": "Internal stop codon at 300208, 300298, 300394, 300415. Nonsense mutation around 300208, 300298, 300394, 300415.", "psc": { "uniref90_id": "UniRef90_A0AAC9T0I9", "product": "DUF1410 domain-containing protein", "uniref50_id": "UniRef50_A0AAC9T0I9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAC9T0I9", "UniRef:UniRef50_A0AAC9T0I9" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAC9T0I9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T0I9" ], "product": "DUF1410 domain-containing protein" } }, "id": "NKIECMJPJD_552", "locus": "GCA000828735_00526" }, { "type": "tRNA", "sequence": "contig_1", "start": 301306, "stop": 301382, "strand": "+", "gene": "trnR", "product": "tRNA-Arg(tct)", "amino_acid": "Arg", "anti_codon": "tct", "score": 78.7, "nt": "GCACCTGTAGCTCAGTTGGATAGAGCATACGCCTTCTAAGCGTACGGTCTGGGGTTCGAATCCCTTCAGGTGCGCCA", "db_xrefs": [ "SO:0001036" ], "anti_codon_pos": [ 301340, 301342 ], "id": "NKIECMJPJD_9", "locus": "GCA000828735_00527" }, { "type": "tRNA", "sequence": "contig_1", "start": 301466, "stop": 301542, "strand": "+", "gene": "trnR", "product": "tRNA-Arg(tcg)", "amino_acid": "Arg", "anti_codon": "tcg", "score": 77.8, "nt": "GCACCCATAGTTCAATTGGTTAGAATATATGCCTTCGAAGCATAGGGTTAGGAGTTCGAGTCTCTTTGGGTGCGCCA", "db_xrefs": [ "SO:0001036" ], "anti_codon_pos": [ 301500, 301502 ], "id": "NKIECMJPJD_10", "locus": "GCA000828735_00528" }, { "type": "cds", "sequence": "contig_1", "start": 301629, "stop": 301898, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00535.33" ], "nt": "ATGTTATTAACAATTGCTTTTTATTTATCAAAGACAACTCATTATCTAAAAAAAAATTTTAATTATTTTTTAGATTTATTAAATCAAAATCAAAAAAATATTGAATTAATTATTATTGATGATGCTAGTGATTATAATTTATTTAAAACATTAAAACCATTAATTGAAAATACTAATGCTAAGATTAAATATTTTTATTTAAATGAAACACAAGGCAATGCTTATGCATATAATTTAGCAACGAAATATGCTCACGGTAAATACATTTGA", "aa": "MLLTIAFYLSKTTHYLKKNFNYFLDLLNQNQKNIELIIIDDASDYNLFKTLKPLIENTNAKIKYFYLNETQGNAYAYNLATKYAHGKYI", "aa_hexdigest": "f0d60c60e7711154fda364d3fcc711b8", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00535.33", "name": "Glycos_transf_2", "length": 65, "aa_cov": 0.7303370786516854, "hmm_cov": 0.9846153846153847, "evalue": 2.083271666487809e-11, "score": 39.6137580871582, "start": 25, "stop": 89 } ], "seq_stats": { "molecular_weight": 10526.976299999991, "isoelectric_point": 9.048347282409669 }, "id": "NKIECMJPJD_553", "locus": "GCA000828735_00529" }, { "type": "cds", "sequence": "contig_1", "start": 302361, "stop": 302567, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAAAAATTTATGAATTATACACAATTAATATTAATTCTAAAAAACAAATTAAAGAACGAACAATGATTATTAGTCATGCCTTATCAAATGCTAAAAAGTATTTAGAGATTTATTTTGCTAATTTTGAGAATAATTTATATGTTCGCAAGTTTAAAATTAATATTTTAAAATATTATTTAAAATCAAAACAGGGATTAAATTAA", "aa": "MKKIYELYTININSKKQIKERTMIISHALSNAKKYLEIYFANFENNLYVRKFKINILKYYLKSKQGLN", "aa_hexdigest": "c1ca994b2b1a2a3d7078282e7589c90d", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8267.709199999996, "isoelectric_point": 9.952709770202638 }, "id": "NKIECMJPJD_554", "locus": "GCA000828735_00530" }, { "type": "cds", "sequence": "contig_1", "start": 302568, "stop": 302771, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTGATAAGTCAACATTAAAACAAGAAAATACGATCGAATATCCCGTTGTTGGTGCACTAAGGCGTGCTAGCGCTAGTGTTTTAGATTATATTTTTATTTCCATTATTGCTTTTTTATTATCTTTATTAGTTTTTATTAATAGTAATTTTAAAATTAGTGATATTTTTGTTAATAATGATGAGCTTGCTCCTGAACCTTGA", "aa": "MSDKSTLKQENTIEYPVVGALRRASASVLDYIFISIIAFLLSLLVFINSNFKISDIFVNNDELAPEP", "aa_hexdigest": "e79cb96ff433d6ab6caca331ea5a8973", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7492.551999999994, "isoelectric_point": 4.507070732116699 }, "id": "NKIECMJPJD_555", "locus": "GCA000828735_00531" }, { "type": "cds", "sequence": "contig_1", "start": 302988, "stop": 303389, "strand": "+", "frame": 3, "gene": null, "product": "RDD family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9X6Z9", "UniRef:UniRef90_A0AAC9X6Z9" ], "nt": "ATGGGATTTTGTTTTGTTTTTGCTGATGCAAGTTTTAAATCAATTCAAGCTATGAATGAGACTGAGCTAAAAGAGTATTATCATCAAATTAAAGATTTAAAAGCTTATGTGCAGTCAATTTTATCTTTAAAAATTAATGATAATTTCACCAATACTACAACTATGGCTATTTTAAGTATTTTAGTTAAAAGTCTATATTCAATTATGGGTTTAGTTAGTTGTATTATTGTAATTCACATGTTTATGAATTCAAAAAAACGTGCTTTACATGATCAAGTAGCAAAAGTTGTTTTATTATGGATGCATTATGAGAAACCAAAACAAGCGCCTAAACATAAAACAAAACGTGAAGTTGAGATTCAAAAAATCGATAATTTAATTCAAGAACTAGATAAAATATAA", "aa": "MGFCFVFADASFKSIQAMNETELKEYYHQIKDLKAYVQSILSLKINDNFTNTTTMAILSILVKSLYSIMGLVSCIIVIHMFMNSKKRALHDQVAKVVLLWMHYEKPKQAPKHKTKREVEIQKIDNLIQELDKI", "aa_hexdigest": "759059ab575e854e489d5f5dcfb839ac", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 302688, "stop": 303389, "observations": { "mutation": [ 302769, 302865, 302955 ], "stop": [ 302769, 302865, 302955 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAC9X6Z9", "query_cov": 1.0, "subject_cov": 0.48717948717948717, "identity": 0.94, "score": 239.0, "evalue": 1.24e-77, "gene_start": 1, "gene_end": 133, "reference_start": 141, "reference_end": 273, "reference_sequence": "MSDKTTLKQENTIEYPVVSALRRAGAGVLDYIFVSILAFLLSLLVFINSNFKISDVFVNNDELAPEPWRLFLMAIIAFFVYCTYYIIIPVFCKSYTLFRWAFSIRMISLVKQNDKKILFVALIKHSLLTWLIFIMINLLAMGFCFAFADASFKSIEAMNEIELKEHYRQIKDLKAYVQSILSLKINDNFTSTTTMAILSILVKSLYSIMGLISCIIVIHMFMNSKKRTLHDQVAKVVLLWMHYEKPKQAPKHKTKREVEIQKIDNLIQELDKI" }, "paralog": false, "identity": 0.9227467811158798, "score": 420.0, "evalue": 1.99e-151, "description": "Internal stop codon at 302769, 302865, 302955. Nonsense mutation around 302769, 302865, 302955.", "psc": { "uniref90_id": "UniRef90_A0AAC9X6Z9", "product": "RDD family protein", "uniref50_id": "UniRef50_A0AAC9X6Z9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAC9X6Z9", "UniRef:UniRef50_A0AAC9X6Z9" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAC9X6Z9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9X6Z9" ], "product": "RDD family protein" } }, "id": "NKIECMJPJD_556", "locus": "GCA000828735_00532" }, { "type": "cds", "sequence": "contig_1", "start": 303414, "stop": 304064, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13086.12", "PFAM:PF13245.12", "PFAM:PF13604.12", "PFAM:PF00580.27" ], "nt": "ATGGGATTAGGAATTGATTATTCACGCTTAAATCAAGAACAAAAAGAAGCAGTTACAGCAGACTTAGGACCGCAATTAGTAATTGCAGGGGCAGGGACAGGAAAAACATCTGTTTTAACTTTACGTATTGCTTATTTAATTACTGAAAAAAATATTCATCCATCACGGATTTTAGGTTTTACTTTTACTAATAAGGCTGCTGATGAAATGAAAGAACGTGTTGGAAAAACAATTGGCGTTTCTATTCCTTATTTAAGTACTTTTCATTCAATGTGTGTAAAAATTTTACAACAAGATATTCATTATTTAAATTATAATAACAATATTAAAATTATTGATACAGACGATCAAGAAGTTTTGCTTAAAGAAATTTTTGATCAATTAAACATTGAAAAAAAATCGCAAGTTATTAAGAAAATAATTAAAACGATTAGTAAAGTTAAAAATAAATTTTTTGATCAAAACGATATGCTAAATGAAAAAAATCACAAGTATTTAGAATTGGTTGATTTAAATGATGCACAAAGATTAGTTGATATTTATAAGATTTATTGTGATCGATGTTTTAAATTAAATTATGTTGATTTTGATGATTTGATCAATTTAACTCATAAGTTATTTATTGAGTTTCCAGAAGTTTTAGAAAAATGA", "aa": "MGLGIDYSRLNQEQKEAVTADLGPQLVIAGAGTGKTSVLTLRIAYLITEKNIHPSRILGFTFTNKAADEMKERVGKTIGVSIPYLSTFHSMCVKILQQDIHYLNYNNNIKIIDTDDQEVLLKEIFDQLNIEKKSQVIKKIIKTISKVKNKFFDQNDMLNEKNHKYLELVDLNDAQRLVDIYKIYCDRCFKLNYVDFDDLINLTHKLFIEFPEVLEK", "aa_hexdigest": "9ae8560e2af1200e759f7441ebf6bc47", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13086.12", "name": "AAA_11", "length": 158, "aa_cov": 0.6990740740740741, "hmm_cov": 0.9177215189873418, "evalue": 1.7126302817105776e-09, "score": 33.356258392333984, "start": 10, "stop": 160 }, { "id": "PF13245.12", "name": "AAA_19", "length": 101, "aa_cov": 0.4583333333333333, "hmm_cov": 0.7128712871287128, "evalue": 1.7522757322953586e-15, "score": 52.91849899291992, "start": 14, "stop": 112 }, { "id": "PF13604.12", "name": "AAA_30", "length": 70, "aa_cov": 0.3055555555555556, "hmm_cov": 0.9428571428571428, "evalue": 1.8557898284312006e-08, "score": 29.855323791503906, "start": 10, "stop": 75 }, { "id": "PF00580.27", "name": "UvrD-helicase", "length": 213, "aa_cov": 0.9537037037037037, "hmm_cov": 0.971830985915493, "evalue": 9.304799194189446e-54, "score": 178.43719482421875, "start": 10, "stop": 215 } ], "seq_stats": { "molecular_weight": 24978.61830000004, "isoelectric_point": 6.526332664489746 }, "id": "NKIECMJPJD_557", "locus": "GCA000828735_00533" }, { "type": "cds", "sequence": "contig_1", "start": 304455, "stop": 305645, "strand": "+", "frame": 3, "gene": "uvrD", "product": "DNA 3'-5' helicase", "db_xrefs": [ "COG:COG0210", "COG:L", "SO:0001217", "UniRef:UniRef50_Q9PPY9", "UniRef:UniRef90_Q9PPY9" ], "nt": "TTGTTAGAAGAAGGAACGCCAGCTAACCAAATCGCTGTTTTGTTCCGAAGCAATCACTATTCTCGTACGATTGAACAATCAATGATGCGCGAGAGCATTCCTTATACAATTCTTGGCAGTAAAAAGTTTTATGAACGTGCTGAAATTAAGGACATGATTGCTTATTTAAAAGTAGTTAATGATTTAGATGAACTATCTTTTTTAAGGATTATTAATACACCACGTCGTGCGATTGGTCCAACTACTTTTGAACATGTTAAACATTATGCAATAAATAATAATTTATTTTTATTTGAAGCATTAGCTGAAGTTGAAAAAAACCATTTAATAAATAACACACAAAAAAAGAATATTTTAAATTTTGTTAATTTAATTAAAGAAATCCGTGATGAAATGGAAGATTTAAAAATTCATGAGATTTTAGAATTAATTTACAAAAAAGTTAACTATGAAGCTTATTTATTAGAAAACGAAAAAGCAGAAGATAAAATCGATAATGTTTATGAATTAAAACGCGCTATGAAAATGTATGTTGATCGTCATCCAGATGACACGATTAATGATTATTTAAATTCGATTGCTTTATATTTGAATAAGGATGGTAAAGATAGTAAAGAAAATGTTTTATTAATGACAGTTCATAATTCTAAAGGTCTAGAATATGAAAACGTTTTCGTTGCTGGTATGAACGAAGGACTTCTTCCTTCTGATCGAGCAATTAATGATGATCCAATTAAAGGAGTTGAAGAAGAACGAAGAATTGCTTATGTAGCCTTAACACGAGCAAAAAAAAATCTTTATATTTCATCTGCTTGTTGTTATGATCCATTAGCAAGAAGACAAGTATTTGAAAGTCGTTTTATTAATGAAATTGGTTTTAATAATTTAAAGATTATAAATAGTAGTTTTAAAAATAATAAACCAGAGGATATGCCATTAAAATCATTCTTAAAACAACAAGAAGAACGTTCATGGTTCGATTCAAAACAAAAACAAAAAGAAGTTGAAGATAATTTTTATCAAACAATGAAAAACGATTTTGAAATTGGTGAAAGAATTGTTCACACAAGTTTTGGTGATGGTGTTATTATTGGAATTGATGGTGATATCTTAACAATTGCTTTCAGTAAATATCGTCAACCAAAACAAATTAATTTTAATCATAAAACAATCGTAAGAAGAATAGGTTAA", "aa": "MLEEGTPANQIAVLFRSNHYSRTIEQSMMRESIPYTILGSKKFYERAEIKDMIAYLKVVNDLDELSFLRIINTPRRAIGPTTFEHVKHYAINNNLFLFEALAEVEKNHLINNTQKKNILNFVNLIKEIRDEMEDLKIHEILELIYKKVNYEAYLLENEKAEDKIDNVYELKRAMKMYVDRHPDDTINDYLNSIALYLNKDGKDSKENVLLMTVHNSKGLEYENVFVAGMNEGLLPSDRAINDDPIKGVEEERRIAYVALTRAKKNLYISSACCYDPLARRQVFESRFINEIGFNNLKIINSSFKNNKPEDMPLKSFLKQQEERSWFDSKQKQKEVEDNFYQTMKNDFEIGERIVHTSFGDGVIIGIDGDILTIAFSKYRQPKQINFNHKTIVRRIG", "aa_hexdigest": "258d64f50209ebbddeac128b4c1a4cb3", "start_type": "TTG", "rbs_motif": null, "genes": [ "uvrD" ], "truncated": "5-prime", "pseudogene": { "start": 304155, "stop": 305645, "observations": { "mutation": [ 304218, 304431 ], "stop": [ 304218, 304431 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PPY9", "query_cov": 1.0, "subject_cov": 0.5329744279946165, "identity": 0.9570000000000001, "score": 744.0, "evalue": 2.95e-265, "gene_start": 1, "gene_end": 396, "reference_start": 348, "reference_end": 743, "reference_sequence": "MGVGIDYSRLNQEQKEAVTADLGPQLVIAGAGTGKTSVLTLRIAHLITEKHIHPSRILGFTFTNKAADEMKERVGKTIGVSIPYLSTFHSMCVKILQQDIHYLNYHNNIKIIDADDQEVLLKEIFDQLSIEKKSPIIKKTIKMISKVKNKFFDQSDIMNEKNRKYLEIVDRNDAQKLADIYTIYCDRCFKLNYVDFDDLINLTYKLFAEFPEVLEKWQNKFDYILVDEFQDTNKIQYDLISLLAAKHQNLFVVGDPDQMIYSFRGAEQWIINNFNQNFKNTKVTILKTNYRSTQPILDTANRLIDANNNSYKKNLTAFNTNDHNIPIYLRGQNQIDEANWIARTVKELLEEGTPANQIAILFRSNHYSRTIEQSMMRESIPYTILGSKKFYERSEIKDMIAYLKVVNDLDELSFLRIINTPRRAIGPTTFEHVKHYAINNNLFLFEALAEVEKNHLINNTQKKNILNFVNLIKEIRDEMEDLKLHEVLELIYKKVNYEAYLLENERAEDKIDNVSELKRAMKMYVDRHPDDTINDYLNSIALYLNKDSKDSKENVLLMTVHNSKGLEYENVFVVGMNEGHLPSDRAIDDDPIKGVEEERRIAYVALTRAKKNLYISSACCYDPLARRQVFESRFINEIGFNNLKIINSSFKNNKPEDMSLKSFLKQQEERSWFDSQQKQKEAEDNFYQTMKNDFEIGERIVHTSFGNGVIIGIDGDILTIAFSKYRQPKQISFNHKTIVRRMG" }, "paralog": false, "identity": 0.9455645161290323, "score": 941.0, "evalue": 0.0, "description": "Internal stop codon at 304218, 304431. Nonsense mutation around 304218, 304431.", "psc": { "uniref90_id": "UniRef90_Q9PPY9", "gene": "UvrD", "product": "DNA 3'-5' helicase", "uniref50_id": "UniRef50_Q9PPY9", "cog_id": "COG0210", "cog_category": "L", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PPY9", "UniRef:UniRef50_Q9PPY9", "COG:COG0210", "COG:L" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PPY9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PPY9" ], "product": "DNA 3'-5' helicase" } }, "id": "NKIECMJPJD_558", "locus": "GCA000828735_00534" }, { "type": "cds", "sequence": "contig_1", "start": 305649, "stop": 305795, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13277.11" ], "nt": "ATGAAAATTTTAGCTATTGGAGATATTTTTTCAAAACAAGGAAGAAAAGCAGTGGCCAATGAATTGCCCAAGCTAAAACAAGAGTATCAAATTGATTTTGTAATTGCTAATGCTGAGAATACAACACATGGTCGCGGTTTGTGTTGA", "aa": "MKILAIGDIFSKQGRKAVANELPKLKQEYQIDFVIANAENTTHGRGLC", "aa_hexdigest": "9b76044b46dbd0a4ca3561f7101ebe28", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13277.11", "name": "YmdB", "length": 44, "aa_cov": 0.9166666666666666, "hmm_cov": 1.0, "evalue": 1.1176827222930704e-16, "score": 56.39116668701172, "start": 4, "stop": 47 } ], "seq_stats": { "molecular_weight": 5331.134800000001, "isoelectric_point": 8.978850364685059 }, "id": "NKIECMJPJD_559", "locus": "GCA000828735_00535" }, { "type": "cds", "sequence": "contig_1", "start": 305985, "stop": 306440, "strand": "+", "frame": 3, "gene": "ymdB", "product": "Metallophosphoesterase", "db_xrefs": [ "COG:COG1692", "COG:T", "EC:3.1.4.16", "KEGG:K09769", "SO:0001217", "UniRef:UniRef50_A0A2C9DYY2", "UniRef:UniRef90_A0A2C9DYY2" ], "nt": "ATGATTCGTGTAACTAATTTATTAGGTAATTCTATTGATATGAAAGGTTTACAAACAAATCCTTTTGAATCTTTAGATAAAATTATTGCTTTTAATGAAGCACCGATTCATATTGTTGATTTTCATGCTGAAACAACAAGTGAAAAAAATGCACTTTTTTTAGATTTTAAAAGTAAATTGTCTTTAGTTTATGGTACTCATACACATATTCCAACAAATGATGCTAGAATTGTCGATCATACGGCTTTTCAAACTGATTTAGGAATGTGTGGTGCTGCTAATAGCGTGATTGGGGCAAATCCAAGTAGTATTATTCAACGATTTCGTGATCCAAATAAACGTTTTATATTAGAACCAAGTGATGAAAAATATGTTTTTTGTGCTAGTTTAGTTGAAATTGATGATGAAACAAATTTTCCAACTAATATTACACCTATTGTAATTTATGAAAAATAA", "aa": "MIRVTNLLGNSIDMKGLQTNPFESLDKIIAFNEAPIHIVDFHAETTSEKNALFLDFKSKLSLVYGTHTHIPTNDARIVDHTAFQTDLGMCGAANSVIGANPSSIIQRFRDPNKRFILEPSDEKYVFCASLVEIDDETNFPTNITPIVIYEK", "aa_hexdigest": "da17ba31d4e02903caca940f7409a080", "start_type": "ATG", "rbs_motif": null, "genes": [ "ymdB" ], "truncated": "5-prime", "pseudogene": { "start": 305685, "stop": 306440, "observations": { "mutation": [ 305793, 305856 ], "stop": [ 305793, 305856 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A2C9DYY2", "query_cov": 1.0, "subject_cov": 0.5741444866920152, "identity": 0.96, "score": 291.0, "evalue": 7.97e-98, "gene_start": 1, "gene_end": 151, "reference_start": 113, "reference_end": 263, "reference_sequence": "MKILAIGDIFSKQGRKAVANELPKLKQEHQIDFVIANAENTTHGRGLCWKHYEELVGYGIDCITMGNHTWDHPDIFEILTTKTNIIRPYNIINTHQYHLVGSGSRVFYCNKKMIRVTNLLGNSIDMKGLQTNPFESLDKIIACNEAPIHIVDFHAETTSEKNALFLDFKGKLSLVYGTHTHIPTNDARIVDRTAFQTDLGMCGAANSVIGANPSSIIQRFRDPNKRFILEPSDEKYVFCASLVEIDDETNQPIKITPIVIYEK" }, "paralog": false, "identity": 0.9601593625498008, "score": 489.0, "evalue": 1.11e-178, "description": "Internal stop codon at 305793, 305856. Nonsense mutation around 305793, 305856.", "psc": { "uniref90_id": "UniRef90_A0A2C9DYY2", "gene": "YmdB", "product": "Metallophosphoesterase", "ec_ids": [ "3.1.4.16" ], "uniref50_id": "UniRef50_A0A2C9DYY2", "cog_id": "COG1692", "cog_category": "T", "kegg_orthology_id": "K09769", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A2C9DYY2", "EC:3.1.4.16", "UniRef:UniRef50_A0A2C9DYY2", "COG:COG1692", "COG:T", "KEGG:K09769" ] }, "pscc": { "uniref50_id": "UniRef50_A0A2C9DYY2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2C9DYY2" ], "product": "Metallophosphoesterase" } }, "id": "NKIECMJPJD_560", "locus": "GCA000828735_00536" }, { "type": "tRNA", "sequence": "contig_1", "start": 306561, "stop": 306635, "strand": "+", "gene": "trnC", "product": "tRNA-Cys(gca)", "amino_acid": "Cys", "anti_codon": "gca", "score": 62.3, "nt": "GGTGCCATAGCCAAGTGGTAAGGTCAGGAGCTGCAACCTCCTTATTCGTCAGTTCGAATCTGACTGGCACCTCCA", "db_xrefs": [ "SO:0000258" ], "anti_codon_pos": [ 306593, 306595 ], "id": "NKIECMJPJD_11", "locus": "GCA000828735_00537" }, { "type": "tRNA", "sequence": "contig_1", "start": 306639, "stop": 306715, "strand": "+", "gene": "trnR", "product": "tRNA-Arg(acg)", "amino_acid": "Arg", "anti_codon": "acg", "score": 74.1, "nt": "GCATCCTTAGCTCAATTGGACAGAGCGTCTGGCTACGGACCAGAAGGTTAAGGGTTCGACTCCTTTAGGGTGCGCCA", "db_xrefs": [ "SO:0001036" ], "anti_codon_pos": [ 306673, 306675 ], "id": "NKIECMJPJD_12", "locus": "GCA000828735_00538" }, { "type": "tRNA", "sequence": "contig_1", "start": 306763, "stop": 306839, "strand": "+", "gene": "trnP", "product": "tRNA-Pro(tgg)", "amino_acid": "Pro", "anti_codon": "tgg", "score": 83.2, "nt": "CGGGAAGTAGCACAGCTTGGTAGTGCACTTGGTTTGGGACCAAGGGGTCGCAGGTTCGAATCCTGTCTTCCCGACCA", "db_xrefs": [ "SO:0000268" ], "anti_codon_pos": [ 306780, 306782 ], "id": "NKIECMJPJD_13", "locus": "GCA000828735_00539" }, { "type": "tRNA", "sequence": "contig_1", "start": 306844, "stop": 306920, "strand": "+", "gene": "trnM", "product": "tRNA-Met(cat)", "amino_acid": "Met", "anti_codon": "cat", "score": 75.1, "nt": "GGCTGGATAGCTCAACTGGTTAGAGCGCTCGGTTCATACCCGGGAGGTTCAGAGTTCAAATCTCTGTCCAGCCACCA", "db_xrefs": [ "SO:0000266" ], "anti_codon_pos": [ 306878, 306880 ], "id": "NKIECMJPJD_14", "locus": "GCA000828735_00540" }, { "type": "tRNA", "sequence": "contig_1", "start": 306923, "stop": 307000, "strand": "+", "gene": "trnI", "product": "tRNA-Ile2(cat)", "amino_acid": "Ile2", "anti_codon": "cat", "score": 72.5, "nt": "GCTTCTATAGCTCAATTTGGTTAGAGCCCCCGACTCATAATCGGTTGGTTACAGGTTCAAGTCCTGTTAGAAGCACCA", "db_xrefs": [ "SO:0000263" ], "id": "NKIECMJPJD_15", "locus": "GCA000828735_00541" }, { "type": "tRNA", "sequence": "contig_1", "start": 307069, "stop": 307161, "strand": "+", "gene": "trnS", "product": "tRNA-Ser(tga)", "amino_acid": "Ser", "anti_codon": "tga", "score": 81.0, "nt": "GGAGAAATACCCAAGTCCGGCTGAAGGGATCAGTCTTGAAAACTGAGAGGCGATGCAAGTCGCGCGGGGGTTCGAATCCCTCTTTCTCCGCCA", "db_xrefs": [ "SO:0000269" ], "anti_codon_pos": [ 307090, 307092 ], "id": "NKIECMJPJD_16", "locus": "GCA000828735_00542" }, { "type": "tRNA", "sequence": "contig_1", "start": 307219, "stop": 307295, "strand": "+", "gene": "trnfM", "product": "tRNA-fMet(cat)", "amino_acid": "fMet", "anti_codon": "cat", "score": 71.8, "nt": "CGCGGGATAGAGCAGCTTGGTAGCTCGTCAGGCTCATAATCTGGAGGTCGGGGGTTCAAATCCCTCTCCCGCAACCA", "db_xrefs": [ "SO:0000266" ], "id": "NKIECMJPJD_17", "locus": "GCA000828735_00543" }, { "type": "tRNA", "sequence": "contig_1", "start": 307298, "stop": 307373, "strand": "+", "gene": "trnD", "product": "tRNA-Asp(gtc)", "amino_acid": "Asp", "anti_codon": "gtc", "score": 58.3, "nt": "GGTTCTGTGGTGTAGTGATTAACATGCCTCTCTGTCACAGAGGAGATCGCGGGTTTGACTCCCGTCAGGACCGCCA", "db_xrefs": [ "SO:0000256" ], "anti_codon_pos": [ 307331, 307333 ], "id": "NKIECMJPJD_18", "locus": "GCA000828735_00544" }, { "type": "tRNA", "sequence": "contig_1", "start": 307375, "stop": 307450, "strand": "+", "gene": "trnF", "product": "tRNA-Phe(gaa)", "amino_acid": "Phe", "anti_codon": "gaa", "score": 79.0, "nt": "GGTTTCGTAGCTCAGTCGGTAGAGCAACGGACTGAAGATCCGTGTGTCGACAGTTCGATTCTGTCCGAAACCACCA", "db_xrefs": [ "SO:0000267" ], "anti_codon_pos": [ 307408, 307410 ], "id": "NKIECMJPJD_19", "locus": "GCA000828735_00545" }, { "type": "cds", "sequence": "contig_1", "start": 308302, "stop": 308613, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATTATTCAAAGTATAATTATTTTTTAGTTCAATTAATTCATCGTATTAAAAATAAAGTTCATTTATTAAAAAATATCATTGTAGTTTTAAATTCAATCAAAATTATTGAAGAAGCTAATAAAAATAGCTTACAAAAAGTGCAAGAAATCACCTTTTTGACTGATGACAAAATTAAAGAATTAAGACAATTTAACCATGATTTTAATGATTTTATTAATAACCAATTAGCTGATTTTATTTATAATTCAAAATTTGTTAATTTAAATAACCTTTATTTAAATAGTTATTATCAACAAGCCTTAAATTGA", "aa": "MNYSKYNYFLVQLIHRIKNKVHLLKNIIVVLNSIKIIEEANKNSLQKVQEITFLTDDKIKELRQFNHDFNDFINNQLADFIYNSKFVNLNNLYLNSYYQQALN", "aa_hexdigest": "e0b1375ce7e995a377de07ae7b23b914", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12417.090599999985, "isoelectric_point": 9.126676368713383 }, "id": "NKIECMJPJD_561", "locus": "GCA000828735_00546" }, { "type": "cds", "sequence": "contig_1", "start": 309455, "stop": 310045, "strand": "+", "frame": 2, "gene": "rpsD", "product": "30S ribosomal protein S4", "db_xrefs": [ "COG:COG0522", "COG:J", "GO:0003735", "GO:0006412", "GO:0015935", "GO:0019843", "GO:0042274", "SO:0001217", "UniRef:UniRef50_Q9PPZ2", "UniRef:UniRef90_Q9PPZ2" ], "nt": "ATGAGTCGTTATACAGGTAGTATTTATAAAAAATCACGCCGTTTAGGATTTTCTTTATTAGAAAATAATAAAGAATTTAATTCTGGTAAAAAAAGAACATACGGTCCAGGACAACACGGTAATAAAAAAGTTAAATTATCAAATTATGGCCAACAATTAGTTGAAAAACAAAAGTTAATGTTTTTATATGGATTGAATGATCGTCAATTTAGACGTTTATATAGAGTTGCACTAGGCCGTCCAGGCGTACTTACTTTAAATTTATTACAAGTTCTTGAATCACGTTTAGATTCATTAGTTTATCGTGCTGGATTTGCTCCAACACGTCGTGCTGCACGTCAATTAGTAAATCACTCACACGTTTTGGTTAACAATAAAAAGGTTAACATCCCTTCAGCACTAGTTGAAGTAGGTAGTACAATTGCTCTTAAGGCTAAATCATTAGAAATACCTTTAATTAAAAATACTTTAAACAAACCAGCTGATTTTATTGAATTAATCGATAAAGAAAAAAAAGTTGCAAAATTAGCACGTTTACCAGAACGTAATGAATTACCAGCAGATGTAAATGAAGCTTACGTTGTTGAATGA", "aa": "MSRYTGSIYKKSRRLGFSLLENNKEFNSGKKRTYGPGQHGNKKVKLSNYGQQLVEKQKLMFLYGLNDRQFRRLYRVALGRPGVLTLNLLQVLESRLDSLVYRAGFAPTRRAARQLVNHSHVLVNNKKVNIPSALVEVGSTIALKAKSLEIPLIKNTLNKPADFIELIDKEKKVAKLARLPERNELPADVNEAYVVE", "aa_hexdigest": "d998240b216863feebdae9c3ed1f3667", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_Q9PPZ2", "query_cov": 1.0, "subject_cov": 0.9702970297029703, "identity": 1.0, "score": 375.0, "evalue": 2.61e-131, "valid": true, "gene": "rpsD", "product": "30S ribosomal protein S4", "uniref50_id": "UniRef50_Q9PPZ2", "cog_id": "COG0522", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015935", "GO:0019843", "GO:0042274" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PPZ2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PPZ2" ], "product": "Small ribosomal subunit protein uS4" }, "genes": [ "rpsD" ], "id": "NKIECMJPJD_562", "locus": "GCA000828735_00547" }, { "type": "cds", "sequence": "contig_1", "start": 310161, "stop": 310430, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAGTTTAATGATAGTGAGAATAATTTAAAAGAAAATGATATTTTAAAAATTAAATATAAAAAAGTAGGACCAAATGAAGTCATCTTTGAAAAAACAGTTCGATTAGCTAATAATTTTGAACTATTATTTGAAATTGAAAATACAAAAAAGAATGAAAAATATGAAATCTTAAGTTTAGAATCAAACAGTAAACATGGATATAATGTTGCGCCAAGTATATTTAATTTTATTTCTAATGATTTACGCACTTTTGAAATCAAAGGTTAA", "aa": "MKFNDSENNLKENDILKIKYKKVGPNEVIFEKTVRLANNFELLFEIENTKKNEKYEILSLESNSKHGYNVAPSIFNFISNDLRTFEIKG", "aa_hexdigest": "ae0b0c2ca60af467fe91e0aafa1c9eb8", "start_type": "TTG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10445.736999999988, "isoelectric_point": 6.589821815490722 }, "id": "NKIECMJPJD_563", "locus": "GCA000828735_00548" }, { "type": "cds", "sequence": "contig_1", "start": 310510, "stop": 310707, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAAACTTAGCTCATTATTAGTAGTTATTTTAGTTTGCGCATTAGTTAAATTTAAATCACTTTGGCTTCCAATAATTAATTCTTTAAAAAGATATTTACGGTTTGATATTAAACCATCTAAAACAAAATTACTATTTTTAATTTCAGCAATTTTTAAAGTATTTGATAGCTTTCAAGAATTATCATTGCTTTTATAA", "aa": "MKLSSLLVVILVCALVKFKSLWLPIINSLKRYLRFDIKPSKTKLLFLISAIFKVFDSFQELSLLL", "aa_hexdigest": "94d24ef72d34d2989e38d4af563c2e5a", "start_type": "GTG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7543.281199999998, "isoelectric_point": 10.106337928771971 }, "id": "NKIECMJPJD_564", "locus": "GCA000828735_00549" }, { "type": "cds", "sequence": "contig_1", "start": 310683, "stop": 311294, "strand": "-", "frame": 1, "gene": null, "product": "DUF1410 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B5ZBZ1", "UniRef:UniRef90_A0A2C9DY09" ], "nt": "ATGAAAAAAAACACACATAAGAAACTTTTGCCTTTAATTTCTATTTTTGTTTTAATCCCTTTGAGTTCACTTGTTGTAATGTGTTCAAAAACTAAAACAAAAATAGTAAAAGAACAAAAGCAAATTACTGCTTCATCGTTAAGATCTACTGATATTGATTTAACATCAATTAACCTTGAAATCTATTTTGCTAAATTTGATGTTAATGATATTTTAGTTAAAAAATTCAGCATTGAATTAGAAGATGATAAGGGAAATAAGATAAATGTTGATATAAATCCTGAATATAACAAAAATTTACAAATGCTAAGTTTTAAATTAGAAAACTTAAAACCAAACACTACATATAAAATTACAAAGTTTAATATCACAGGACATGAGGTTGATCTAACTCAAATAAAAGATTCATTATTCACTACTAAAGCTGAGAATCTTTTACCTGATATCCCTAATTTACCTAATAGTGTTGGTATTGAAATTAAATATATTAAAACTAATTCACTTAGCAATGATGCAACAAAAGTGAATGTTAATGTCAATTTAGAAATTAACCAATCAACATTAGAAAATCAATATGCCCGATTAGTTTATAAAAGCAATGATAATTCTTGA", "aa": "MKKNTHKKLLPLISIFVLIPLSSLVVMCSKTKTKIVKEQKQITASSLRSTDIDLTSINLEIYFAKFDVNDILVKKFSIELEDDKGNKINVDINPEYNKNLQMLSFKLENLKPNTTYKITKFNITGHEVDLTQIKDSLFTTKAENLLPDIPNLPNSVGIEIKYIKTNSLSNDATKVNVNVNLEINQSTLENQYARLVYKSNDNS", "aa_hexdigest": "55caca47731c7371ad230d87599d1485", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 310380, "stop": 311294, "observations": { "mutation": [ 310685 ], "stop": [ 310685 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A2C9DY09", "query_cov": 1.0, "subject_cov": 0.5384615384615384, "identity": 0.995, "score": 377.0, "evalue": 1.88e-129, "gene_start": 1, "gene_end": 203, "reference_start": 1, "reference_end": 203, "reference_sequence": "MKKNTHKKLLPLISIFVLIPLSSLVVMCSKTKTKIVKEQKQITASSLRSTDIDLTSINLEIYFAKFDVNDILVKKFSIELEDDKGNKINVDINPEYNKNLQMLSFKLENLKPNTTYKITKFNITGHEVDLTQIKDSLFTTKAENLLPDIPNLPNSVGIEIKDIKTNSLSNDATKVNVNVNLEINQSTLENQYARLVYKSNDNSWKLSNTLKITEIKNSNFVLDGLISNRKYLFKELIIGSQSDLNLTNAQTKITTNNELSFTTAPKPVEIKSVLIDTKFDNHPSSLINLKFNDSENNLKENDILKIKYKKVGPNEVIFEKTVRLANNFELLFEIENTKKNEKYEILSLESNSKHGYNVAPSIFNFISNDLRTFEIKG" }, "paralog": false, "identity": 0.9901315789473685, "score": 581.0, "evalue": 4.53e-212, "description": "Internal stop codon at 310685. Nonsense mutation around 310685.", "psc": { "uniref90_id": "UniRef90_A0A2C9DY09", "product": "DUF1410 domain-containing protein", "uniref50_id": "UniRef50_B5ZBZ1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A2C9DY09", "UniRef:UniRef50_B5ZBZ1" ] }, "pscc": { "uniref50_id": "UniRef50_B5ZBZ1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B5ZBZ1" ], "product": "DUF1410 domain-containing protein" } }, "id": "NKIECMJPJD_565", "locus": "GCA000828735_00550" }, { "type": "cds", "sequence": "contig_1", "start": 311902, "stop": 315432, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF07198.17" ], "nt": "TTGGTATGGCAACAAAAAATACTTGTTGATCGTCCTAATGTCTTTAAAGTTAATTTTAATAAAGAAGGCGATAAATTTTTTGAAATTAAATTAAACCATGAATTGAGTAATCAGTATTTGACTGCGCATTTTATTAATCAAAATAATGCTAAATATATTCAAAATATTCAGGTTGATCATAATGGTAATGTAATTGTTGGAACTAAACAACTACCTTTAGGAAATTTTTATAAATTCGTAGGTTTAACTTTAAAAGAAGATAATAAAGACACAAAAATTGTTAATTTAGATGAAATTAGTGAACCCATTCGTGGTATTGATAAAACACGCAGTTTTGATGATCAAGGAAACGTTATTATTAACGCACGTGTTTCACAAAATTTAGCTAATCAAAATATTAATGCCTTATTTGAAGATGAAAAACACCAATTACACACTTTAAAGGCAATAGTTGGTACTGATGGTCTTGTGCGTTTATTAACAAAAGATTTAGAAAATCACCATGATTATAAACTAATTCAAATTGTTGATAAGGATAATCATGATGTTATAAAACCTAATAAATTAGATGCGGCACATCAAAATATTGTTCATAAACCAATAAAAATAAAAATTGGAACTGATAAAAATGGTTTACAAAAAGCACTTCATATTCAAGTTGATTCAAGTTTAAATGATCGATTATTAACACTTAGTTTTGAAAACGAAAACCACGATAAAACCAATATTATTGCTAAGGTTAATAATGGTGAAATTACTGTAGATTTAAACAATTTAAAAGATGGACACTTATATAAACTAGATAATATTAGCATTAATAATACCTCTCCAGCAAAACGCGTTGCTAACTTAGATGATTTAAATGAAAGTGCTAAAACTATTAATAAATTAAACACTAATGCATTAAATGATCCATATCATTATGATGATAAAGGGAATTTAATTTTTAATGCTAAAGTAGATTCTAAACAATTCGCTAATGATCAAGTTGAAGCCGTGTTTATTGATGAAAAAAATAATGAACATAAAGTGTTGACAAAGGTTGATCTAAATGGTAATATTGTAATTAACACTTCTGATTTAAACTTAAATCATACATATAAACTAAAACATGTTATTAGTTTAGAAGAGCCAGAAACAAATGTTTTAAATGATTATGAACTTGCAATTAATCAAAAAACAAAGTTTAGTAAACCAGCTGCGATTGTTGGGACATTAGAAGATAATAATTATAAAATTCAACTTTTAAACCCTGATTTAGCTAATAAGTCTGTTGTCTTAACTTTTGAAGATGAAAATCATCATCCTTATCAATTAACTGGTAAATTAAATGATCAACAAGAATTAATTTTAAGAACAAATGATGATCATTTACTACCTAATAATCACACTTATACATTGACTAAAATTACTAATGTTAATAATCAACCAATCGTTAATGTTAATGATTTAGATAGAAAAGATAGATTATTAGAAAAAGTCGATGTAAATAAATTACAACAATTAAATACATACACCTATGATGATTTAGGAAATTTAATTATTGACATTAATGATAATTTAAATAAAATAACAACTCAAGATGCTGTTGCTCATTTTATTGATGAAAATAATAAAGATTATTTTGTTCCTACTAAAGTTGATTTAAATAAGAATAAAATAAGTATTAACACAAAAGATTTAGATGCAAATCACCACTATCAATTGTTAAATATTGTTGATAAAAACAAGCATGAGCAAATTTTATTAAATTCAAACGAAATTAATCCTTTAAACAGAATTAGCATTATTAAACCACTTGCAATTATTAAACAAGATGCAAATAAAAATTTAAACCTACTAACTTTTGATAAGAATTTGTTTAATCGTTCATTAGATGCAACTTTTATTGATGAAAATCAAAAAGAATATCATGTTAAAGCAAAAACTATTATTGATTCATTAGGTCAAACACGATTTATAACTAGTGATGAGGTTTTACCTAACCATCATAAATATCAACTAATTTCTCTTAAATATGATGGTCAAAAAGTAATTAATGTTGCTGATTTGGATCAAAAACAAATTATTGTTAACAAACTAAATATTGGTAAAATTGTTAATAACCAATTAGTTTTTGATGTGCCAAAAAATGTTGAAGAAGGCGAAGATGTTTTTGTTAATTACCAAGATGTTGATGGTAATTTGATAAAAATTCCTGCAATTGTTAATAATAAACACCAACTACAAATTGATCTATCAAACAACCCTAATTTTACAAAAGATAAGATTTATCATTTTGATTCAATTAGCATAGAAGATGAATATCACATTCCTAAAGAAACAATTATTAGTTCAGATGATGTTGATAATTCAGTTAAAACATTAAATAATGAAGTTGATCTGGGCCGTAAAATTATTGTTAAAAATCCTAGTGTTATTCAAAACAAACCCACAACTTTAATTTTAACAGTTAATGACCCTAATAAATTAGTTGCAAATGAACCATTAAAATTAACACTTGCTTTAAAAAACGATCCAAAACATTGTGTTAGTGCTTATGGCTTAATTGATTATAAAAATAATAGTTTAGTTTTTGATTTTAATCATTTACAAACAGATACTGCTTATTACATTAAAAATTTAGTTTTTAAAAATAATAATTTAAATCTTACTGATTTAAACGCTGAAGCTCATAGTCAAACTAAAATCAATATTATTAAAGATCAAGTTATTGGTTATACATTTAGAATTTTTTCTACTAATACATTGGTATTAGAGGATTATCATCAATATGATACTGATCCTAATGCAACTAAATTAAATTTAAATATTAGTACAACTAATAAGCACCCCAAAACAATTACTTATCGTTTATTAGATGAAAATAATAACGCATTAGAACCAGATTTAGTTGCTAACCTAAATTATGATTCAGCTAAAAAAATTTATGTAACACATTTACAAAATTTAAAAGGTAATAGTAAATATTTATTGTTGGGTTTTAATGATGAAAACAACAATAAAATTCATTACTCAACTCCAATTAAAACTCCATTAATTTTTGGCACAACTCCACATGCTACTACAATTAATTTTGATGTTAACAATCCTAAACAAATTATTAAATCACAAGATGCACGAATAGCAATTGATTTTGTAGATCAAGATCAAAAATTAAGCATTGGTAACCCAGTTGAATTAACTTATTTAAATAAGAATAATCAACAAGTTTATAAAACAATAGGGTATGTTAAAGGAATCAACAAAACTAACAAAAGTGGTACGTTAGAATTTGAACTTAATAATTTAGTATCTAATAATAATTATGAATTGTTAGAATTAAAGGCCTTGGGTTTAGCAAATGATGCTTATCAAAAGGTTGTATTTGCTCCTTTTGATCAAGAACATAATCAATTTAGTACGAAATTAATTGGAACATATATTGAATCAATTAGTTCAAATGAAGTAATTAAAAAAGATGAAAATAAACTTGATGTTAATGTAAAAATTGCTTTCTCACAAAAAGATAAAACATTAAATAACGTTTGA", "aa": "MVWQQKILVDRPNVFKVNFNKEGDKFFEIKLNHELSNQYLTAHFINQNNAKYIQNIQVDHNGNVIVGTKQLPLGNFYKFVGLTLKEDNKDTKIVNLDEISEPIRGIDKTRSFDDQGNVIINARVSQNLANQNINALFEDEKHQLHTLKAIVGTDGLVRLLTKDLENHHDYKLIQIVDKDNHDVIKPNKLDAAHQNIVHKPIKIKIGTDKNGLQKALHIQVDSSLNDRLLTLSFENENHDKTNIIAKVNNGEITVDLNNLKDGHLYKLDNISINNTSPAKRVANLDDLNESAKTINKLNTNALNDPYHYDDKGNLIFNAKVDSKQFANDQVEAVFIDEKNNEHKVLTKVDLNGNIVINTSDLNLNHTYKLKHVISLEEPETNVLNDYELAINQKTKFSKPAAIVGTLEDNNYKIQLLNPDLANKSVVLTFEDENHHPYQLTGKLNDQQELILRTNDDHLLPNNHTYTLTKITNVNNQPIVNVNDLDRKDRLLEKVDVNKLQQLNTYTYDDLGNLIIDINDNLNKITTQDAVAHFIDENNKDYFVPTKVDLNKNKISINTKDLDANHHYQLLNIVDKNKHEQILLNSNEINPLNRISIIKPLAIIKQDANKNLNLLTFDKNLFNRSLDATFIDENQKEYHVKAKTIIDSLGQTRFITSDEVLPNHHKYQLISLKYDGQKVINVADLDQKQIIVNKLNIGKIVNNQLVFDVPKNVEEGEDVFVNYQDVDGNLIKIPAIVNNKHQLQIDLSNNPNFTKDKIYHFDSISIEDEYHIPKETIISSDDVDNSVKTLNNEVDLGRKIIVKNPSVIQNKPTTLILTVNDPNKLVANEPLKLTLALKNDPKHCVSAYGLIDYKNNSLVFDFNHLQTDTAYYIKNLVFKNNNLNLTDLNAEAHSQTKINIIKDQVIGYTFRIFSTNTLVLEDYHQYDTDPNATKLNLNISTTNKHPKTITYRLLDENNNALEPDLVANLNYDSAKKIYVTHLQNLKGNSKYLLLGFNDENNNKIHYSTPIKTPLIFGTTPHATTINFDVNNPKQIIKSQDARIAIDFVDQDQKLSIGNPVELTYLNKNNQQVYKTIGYVKGINKTNKSGTLEFELNNLVSNNNYELLELKALGLANDAYQKVVFAPFDQEHNQFSTKLIGTYIESISSNEVIKKDENKLDVNVKIAFSQKDKTLNNV", "aa_hexdigest": "38cf49038243777e022a2b22228f7b1f", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07198.17", "name": "DUF1410", "length": 59, "aa_cov": 0.050170068027210885, "hmm_cov": 1.0, "evalue": 1.4954836565397836e-88, "score": 286.73394775390625, "start": 126, "stop": 184 } ], "seq_stats": { "molecular_weight": 134202.44750000056, "isoelectric_point": 6.00028781890869 }, "id": "NKIECMJPJD_566", "locus": "GCA000828735_00551" }, { "type": "cds", "sequence": "contig_1", "start": 315625, "stop": 316608, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATAAAAAATGGCACTGATTTAAGTCATGGTTTTAAAACAGAACCAACAGGAAGTATTAGTTTAAAAAATAATAGTTTACAAGTAATTAAACGTACTAATGATAGTATTAGATTGTCAATTCCATTAGTTGATAATGATCATGTCTTAGTTGATAGCGATGGTAGTTTATTTACTATTAAAGTTAGTGATTTAGATGATCCTAATAATCTTTTTGATGCAGATAATCAAAAAATAGTGATTGATCAAAACGGAAATCGTACTTTAGAGTTTGATGTTCCTAATGTTTTATTAAATCATAAATATCATATTAACAATATCACATTGACTAATAAACCAATTAATGCTGCTTTTAATCAAAATAAAAATCATGAACAAGTTTATGAGCTAAATAATTCTCAACCACAATTAAGTGAATTCGGTAATGAATTATTAGTTAATACTTATAATTATGAGCCAAATATAACAGATGCAAAAACAAATGTTTTAAATTATAAAGTTAAATTAACCCTTAAACGTTCATTGAATTTAATGAATGAATACTATTTAAAACTAAAGTATATTGATAATAATGGTGATGTTGTTTACTCTGATCCTGTTTCAATCAATAATGCTAATAATGAATATGAATTTAATTTACCGGACAAAAACGCATTAAAATCAAACCGTATTTATAAATTTGATGGGTTATATTATTTTAAAGATCTTAATAATGTTAAGATAACATCAACTAATAAGGTTAATATGAACAATATAACACCTTTACAAATTCAAACAGCTTCAAAAATCAATCTTGATTCAGCAAAAGTAACTATTAATAACATCACCCCTGCTAGTGCAGATTTATACTTACCATTAGTATCAACAGATGATATTTTTGTAAAAGATCAAGTTATTGATGTTACTATTGGTAATAAAGATAATGCGAAAAATGATTTAATGTTTACATCTAATTTAGAATATGATAATATAACAAAAACATGA", "aa": "MIKNGTDLSHGFKTEPTGSISLKNNSLQVIKRTNDSIRLSIPLVDNDHVLVDSDGSLFTIKVSDLDDPNNLFDADNQKIVIDQNGNRTLEFDVPNVLLNHKYHINNITLTNKPINAAFNQNKNHEQVYELNNSQPQLSEFGNELLVNTYNYEPNITDAKTNVLNYKVKLTLKRSLNLMNEYYLKLKYIDNNGDVVYSDPVSINNANNEYEFNLPDKNALKSNRIYKFDGLYYFKDLNNVKITSTNKVNMNNITPLQIQTASKINLDSAKVTINNITPASADLYLPLVSTDDIFVKDQVIDVTIGNKDNAKNDLMFTSNLEYDNITKT", "aa_hexdigest": "afc3d48c9a193c46598c80c5750aa27d", "start_type": "GTG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 37094.05400000004, "isoelectric_point": 5.185671424865721 }, "id": "NKIECMJPJD_567", "locus": "GCA000828735_00552" }, { "type": "cds", "sequence": "contig_1", "start": 317950, "stop": 318627, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF07198.17" ], "nt": "ATGCTTGTTGAATTATGTTTTGAAAATTTAACAGATAAAAACCAACCGGTTTTAAAAACACAAACAGCATTAGTTAAAAAAGATGAACATAATTTTGGCGCTTCAGGGGTAATTACTAATTTATTAGCGGATCATACTTATCGTTTAACAAGTGTTAGTTTAAAACAAAAACCACAATTAGCTAATGTTAATATTAATAACAACAATAATAATGAAATTCTTTTAAACAATATTGAAGATAATCAAAAAACTATTCATACATTATCAAAATCAATTGTTAATGAAATTAGTAATTTAGCAGATGCTTATCCAAAAAATAATAATGGCATATATGATGTTAAATTCAATATTAATATTATTAAGAATAGCAATAAACTTAATAATAAATATGTTAAAGTAGTATTTGAGGACAATGAACACCAACTAATAAGTACAAACGGTCTTTTAGTTAATAAATTAGACCAAACTAATTTAATTAGTTTACAAAATTTTAGTTTTAGTAATTTAAAACCAAATCACCTTTATCGTTTATTAAAAGTTGTTTATGGTGATGAACAAAATTTTGATGCTATTAATGAACAAAAAAATATTTTAGCTTTAAATCCCTCTTTAGTTAATTCAAGCTTTAGTACTACGCCAGCAAAAATTAAAGTTAGCAAAAACGCTATTGATGTATGA", "aa": "MLVELCFENLTDKNQPVLKTQTALVKKDEHNFGASGVITNLLADHTYRLTSVSLKQKPQLANVNINNNNNNEILLNNIEDNQKTIHTLSKSIVNEISNLADAYPKNNNGIYDVKFNINIIKNSNKLNNKYVKVVFEDNEHQLISTNGLLVNKLDQTNLISLQNFSFSNLKPNHLYRLLKVVYGDEQNFDAINEQKNILALNPSLVNSSFSTTPAKIKVSKNAIDV", "aa_hexdigest": "13c3850e0f9e9d57820e8a713932997e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07198.17", "name": "DUF1410", "length": 64, "aa_cov": 0.28444444444444444, "hmm_cov": 0.890625, "evalue": 3.134587555814553e-08, "score": 29.473844528198242, "start": 124, "stop": 187 } ], "seq_stats": { "molecular_weight": 25349.39410000002, "isoelectric_point": 8.490889167785646 }, "id": "NKIECMJPJD_568", "locus": "GCA000828735_00553" }, { "type": "cds", "sequence": "contig_1", "start": 318640, "stop": 319125, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTAAATTAATTTTAGATGATAGTGATAATCAACTACATAGCGGTGATGAAATTAATATCAATTATCGTATTAAAGGAACACAAAATATTATTTCAACTCCAGCTACAATTAGTGATCAAGATAAAAGATATATAAGATGCATGGCAACTAATTTAATTGCTGGTCTTGATTATGAAATTGTGAGTGTTACAATTAAGAATCAAAAAACCAAGAATGTTTCTCCTATCATTTTTGAACTACCATCAGGACCAATTGGTTTTTATACTCTTGCCCCTGTTTTTAAAATAACAAGTCTAAGTATGGATCCTGTTTATCATGAAAATAAAAACACAGCAGATTTAAAAATAAATTTACGAATCGAAAACATTGGCGCTTCTTTATTTAATAAAGATATCAAATTTATTTTCAAACGTAAAAATAATGGTAAGCAAATTTCATTTATTCATAAAGTTACATCAGCAAATGACGCAAATTATGAATGA", "aa": "MIKLILDDSDNQLHSGDEININYRIKGTQNIISTPATISDQDKRYIRCMATNLIAGLDYEIVSVTIKNQKTKNVSPIIFELPSGPIGFYTLAPVFKITSLSMDPVYHENKNTADLKINLRIENIGASLFNKDIKFIFKRKNNGKQISFIHKVTSANDANYE", "aa_hexdigest": "93f8ed2ce1eea98b575b253079c8c293", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18194.650399999995, "isoelectric_point": 8.906194496154786 }, "id": "NKIECMJPJD_569", "locus": "GCA000828735_00554" }, { "type": "cds", "sequence": "contig_1", "start": 319993, "stop": 320259, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTGGTTATTAAAAATAATGTAAACCCACAAACAATAAACACTAAACCATCAAGTACGTATGTTGATTTAAAAGCACAAAATGCAGATGAAAATAAAATCACTTTAAGTTTATTGTTACATTCAAATGACCAAATTTTTGAAGATAAGAATAACCATCTAAAAATCGCTAAAATCAGTATTGATGAACTTGATGCACATGATCAAATAATTAATTCAACAACGCATGATTATGATTTAACATTAGAAAAAGAAAATAATGAATGA", "aa": "MVVIKNNVNPQTINTKPSSTYVDLKAQNADENKITLSLLLHSNDQIFEDKNNHLKIAKISIDELDAHDQIINSTTHDYDLTLEKENNE", "aa_hexdigest": "8824bc062b30186d07fc455d93b2933d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10030.97959999999, "isoelectric_point": 4.865554237365722 }, "id": "NKIECMJPJD_570", "locus": "GCA000828735_00555" }, { "type": "cds", "sequence": "contig_1", "start": 320308, "stop": 320940, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAAAAAAGTTTGGTTCACAAGCAAACCAAGTGATACTATCTATAATGGCGTTAATAAGGACAATATAGTTTATCCAATTAATAATCATAATACTAATATTGATTTAAAAACTTTAGAAAAATCAACTTTAAATAGTGTTAAACCAACAAAAACAAACTTTAACAAGAATGAATCAATTAAGTTAATGGTTGGTTTTAATAAAACAGGTTCTAGTTTAGAAAACAAATATGCGAAATTAGTTTATAAAGATAATAATAACCAACAAATTGAATCAGATCCTGTATTGTTGAATTCAGATACAACTACAAAACCATTTAATAAAGAATTTAGTTTTAATAATAATCAAACTAGCTTAGTTGCTAATCGTGATTTTGAATTTGTTAAGTTAATTATTAGTGATACTGCTGATTTTAAAAACGCTAATACACTTGATTTAACACCTAATTTTAATAAAGATGATGCTAAATTTAGTATCGAACCTACACCTATTAGTGTTAATAATGTTATTCAAAGTGGATCAAATACGTATGATGAAATCCATCTATCATTCGATTATGATGATCAAGATCATAATTTAGTTGATAATGACCAAATTACAATTACATATCGCAAAAAAGGTGAACAAGCTTGA", "aa": "MKKVWFTSKPSDTIYNGVNKDNIVYPINNHNTNIDLKTLEKSTLNSVKPTKTNFNKNESIKLMVGFNKTGSSLENKYAKLVYKDNNNQQIESDPVLLNSDTTTKPFNKEFSFNNNQTSLVANRDFEFVKLIISDTADFKNANTLDLTPNFNKDDAKFSIEPTPISVNNVIQSGSNTYDEIHLSFDYDDQDHNLVDNDQITITYRKKGEQA", "aa_hexdigest": "b39b5bb386819f2328d928f4e80a0bc6", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 23949.185200000007, "isoelectric_point": 5.417403984069824 }, "id": "NKIECMJPJD_571", "locus": "GCA000828735_00556" }, { "type": "cds", "sequence": "contig_1", "start": 321385, "stop": 321993, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTAAAATCACAAATTACACCTAATCATCAATATGAGTTGAGTGAAATTTGTGCAATTAAAAACAAGGGTGCAAACAATGAAATTAAAAAAGATTTAAATGAAAATCATCTATCATTAGCTTTAGGGTTAGCGCCAAATAAAATGGATGTGCAAAAACCAACAATTACAATTACAAAAGTTCAAGATAATCAATCTGTTAGTGCACATGTTAAATATATAATTAATGATCCTGATGGTATTTTAAATTCAACTGATTGCTCAAAAGAATTATTAAATGTTAAATATGCTTTATTTAATCAAACGCAAAATGCTAATTTAATTAACAATATTACAATTAAATATGATCAACAAAATAAACAATTCTATTGTGAATTTGATATTGATAATTTAACTTTAAATCAAGATTATGGTATTTTTGAAATTAGTTTTGCTAATAAACCAATTCATGCCGCATTTGCTAAAATTAATGATACCCAACATCCTTTAGCTCTTTTAAGTACTAATAATCAAGATATAAATCAAAAAATTGCTTTAGATTGTGAAGTTAATAAAGTTACAATTAATAAACAAGATCTTGATTATGATCAAAACACCAAGATTAGTTGA", "aa": "MLKSQITPNHQYELSEICAIKNKGANNEIKKDLNENHLSLALGLAPNKMDVQKPTITITKVQDNQSVSAHVKYIINDPDGILNSTDCSKELLNVKYALFNQTQNANLINNITIKYDQQNKQFYCEFDIDNLTLNQDYGIFEISFANKPIHAAFAKINDTQHPLALLSTNNQDINQKIALDCEVNKVTINKQDLDYDQNTKIS", "aa_hexdigest": "05475d606c6c0aca030b1aec0f2eef55", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 22883.50200000001, "isoelectric_point": 5.487145423889159 }, "id": "NKIECMJPJD_572", "locus": "GCA000828735_00557" }, { "type": "cds", "sequence": "contig_1", "start": 322123, "stop": 323145, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGAGATTGGTCATGACAAATTAATTGCTAATAAGGCTTATGAAATCCAAGCTATTTATTTCTCAGACCAAAATAATGATTTTGCATGCACAAACACAAATCAAATTCCAATTAATTCATCAATTACAAATAAAAAAATAGTTATAAATCCATCAGCAACAACAATTAGTTCACATGAAGTTAAAAATATTACTTCAACATCAGCTGATATTAATTGTTCTATAAGTACAAATGATGGTGATATTTTTAAACCAAATTCACAAATTATAGGTCATTTTGCTAAAGGTGATGATTTAACTAAGACTTTTGTAGCTAATGGTGTTGTTGCGAATGTAAATAACCAAACAAAACTTAGCTTTACGTTAAATAATTTAGAACCAAATACTAAATATACATTAGTTAGTGTTGAATTTAAACAACAACCAAATAATGCTTATAAACATATTAATAACAAATATAATGATCAGTTTTATTCAAATGATCAAACACATTCAATTATTTTTACAACAAGTACACCTATTTTTGAAATTAATAGTATTAAAACTATAAAAAATACTTACGCATCAAATAAAGAAGAAATTAAAGTTAACGCTAAATTTAATAAAATTGATCCATATTTAAATGCTAAAAAACTAAAATTAGTTTATCAATCACAAAATAATAACGAAACCTTTGAAACTCAACCATTATTAGTTAGCTCAAATACACATGATTATGTGTTTAGTTTTGATAAAAACGCTTCAAATTCAATTATTGGTAACCGTGAATATCATTTAGATAAAATTTTATATTTAAATGAACATAACCAATGGTGTGAATTAAATAAGAATAATCTTGTTGACCAATTTAATGTTGAACCTACACCAATTAGTATTGCAAAAATTGTTGAAGATGATTCAAAAAGAACACAAAACGATGTTAAATTTAGAATTTATTTTAATGACTTAAATCAAGATCAAATCTTTAATGATAATGATAATGTTAATGTTAAGTTAGTTTATCCAAATACAAACAATGTATGA", "aa": "MEIGHDKLIANKAYEIQAIYFSDQNNDFACTNTNQIPINSSITNKKIVINPSATTISSHEVKNITSTSADINCSISTNDGDIFKPNSQIIGHFAKGDDLTKTFVANGVVANVNNQTKLSFTLNNLEPNTKYTLVSVEFKQQPNNAYKHINNKYNDQFYSNDQTHSIIFTTSTPIFEINSIKTIKNTYASNKEEIKVNAKFNKIDPYLNAKKLKLVYQSQNNNETFETQPLLVSSNTHDYVFSFDKNASNSIIGNREYHLDKILYLNEHNQWCELNKNNLVDQFNVEPTPISIAKIVEDDSKRTQNDVKFRIYFNDLNQDQIFNDNDNVNVKLVYPNTNNV", "aa_hexdigest": "0042052b89c26963ca7b68972a9a1158", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 38792.5532, "isoelectric_point": 5.917189216613769 }, "id": "NKIECMJPJD_573", "locus": "GCA000828735_00558" }, { "type": "cds", "sequence": "contig_1", "start": 323845, "stop": 324012, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTTAATACAAAAATAAATCCAACTACTCCAAAACAAAAAAACATACGTGTTAGTGTTAAAACAATTCAAAATAATGAAGTAATTTTAAATGATGTCGATCTTAGCGGATTAAGTGAAAATACAGAATATGAACTAAAAACGGTTAGTTTAAATCAAGAAAATTGA", "aa": "MFNTKINPTTPKQKNIRVSVKTIQNNEVILNDVDLSGLSENTEYELKTVSLNQEN", "aa_hexdigest": "657922616a265c87993393e446c497f5", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6263.9490000000005, "isoelectric_point": 5.144690513610839 }, "id": "NKIECMJPJD_574", "locus": "GCA000828735_00559" }, { "type": "cds", "sequence": "contig_1", "start": 324079, "stop": 324345, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAACACTTATTTATTAATAAAATTGATATTAACAAAAAAGCCCAAGAAAACAGTTATACCCTAAAATTAACTTTAGATGGTGATTATTCTGAATTTATTAAACAAGGTTATTTACGTTTATTATTAGTAAATAATAAAAACGAAGAGTTTAATTATACAAAATCACATAAAGATACAGATAAAAATCAAGATGATAATGCTGTTAAATACTTTACTTTTGACCTTAATAATTTAACATGGGGAATGCAATATACAATTAAATGA", "aa": "MKHLFINKIDINKKAQENSYTLKLTLDGDYSEFIKQGYLRLLLVNNKNEEFNYTKSHKDTDKNQDDNAVKYFTFDLNNLTWGMQYTIK", "aa_hexdigest": "5ddae78c1019751c9cb981c8b266af98", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10532.752799999991, "isoelectric_point": 7.893847465515138 }, "id": "NKIECMJPJD_575", "locus": "GCA000828735_00560" }, { "type": "cds", "sequence": "contig_1", "start": 324346, "stop": 324621, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00331586B6", "UniRef:UniRef90_UPI00331586B6" ], "nt": "GTGGGTTTAGCAAAAAATAATGAGAGTGATAATACTGATTTTAAAAAATACAAATTAGAAAATGGTTATCTAACAAATAAAAAAGACGATTTAGATCAATTTACAGTCCGTGCAAGTAAATCTTTAAAAATTAAGCAAGTAAATGTTAGTGAAATCAAAGCACATCATGCAAAACTAAATCTAAAACTTGAAGACCAAGATAATGTTTTAAAAAATCTAAATTATGAGCTTGAATTACGTTTTGGAGCAAAAAATAATCAATCATCTAATTCATGA", "aa": "MGLAKNNESDNTDFKKYKLENGYLTNKKDDLDQFTVRASKSLKIKQVNVSEIKAHHAKLNLKLEDQDNVLKNLNYELELRFGAKNNQSSNS", "aa_hexdigest": "9dc23aa4c53edbbbee324ef0857c5a27", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00331586B6", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.9890000000000001, "score": 170.0, "evalue": 1.62e-53, "valid": true, "uniref50_id": "UniRef50_UPI00331586B6" }, "pscc": { "uniref50_id": "UniRef50_UPI00331586B6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00331586B6" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10457.587499999992, "isoelectric_point": 9.126482963562015 }, "id": "NKIECMJPJD_576", "locus": "GCA000828735_00561" }, { "type": "cds", "sequence": "contig_1", "start": 324703, "stop": 324951, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAATATGAACTAAAAGATGCTAAATTAATATTTACTGAAGAAGAATTGAAGAAATTTAACAAAGTAACAGCAGTTAATAATTTAAATGTTGATTCTGATCAAGTAATGTTTAAAACAATGAAAAGTGCATATGTTGAAACTGCAACAATTAATTTTGTAAATAATACTCCAAACGGTACAATTGATCTTAAACTAAATGGGGATTTAAGTGTATTTAGTAAAAGTCAATATATTCGTTTTGAGTGA", "aa": "MQYELKDAKLIFTEEELKKFNKVTAVNNLNVDSDQVMFKTMKSAYVETATINFVNNTPNGTIDLKLNGDLSVFSKSQYIRFE", "aa_hexdigest": "d6c817d0d3fb1f2c1b22a0a8e9125056", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9432.6279, "isoelectric_point": 5.389382362365722 }, "id": "NKIECMJPJD_577", "locus": "GCA000828735_00562" }, { "type": "cds", "sequence": "contig_1", "start": 325207, "stop": 325728, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAACTCCAACTAATTATTCACTATTAACACAAGAAGTTAATGAGAATGCAAATAAAGTTAAAAAAGCATTAGGGGCTTTAGACTTATGTTTTGAGTTGAATGATCCAAGTAATATATTAACTAATCAAGATCAATTTAGTATTAAATACGCAATGTTAAACAATCTAAAACAAAGCGTTTCAGCGACTGGACAAGTTGAAATTAAAGAAAATAAAAAAATAGTTAAATTCCATATTAGTGGAATTGATCTAAATCAAGATTATAAAGTTTGCGAGTTAATGTTGTTGACAAAACCAGCTAGAATGGGTGAAAAAATCAATAATAATGATAAAAATAACTATTCATTTATTTATCAAAATTCAACAAACGATGATAATCAATATAAATTTAAAATTTTATTAGAAAATAATGATTTATCAACTAAAGAACGTCCATCAAAAACAGGTGATATTGTTAATAATAAAGGTAAGGTTGATATTACAATTACCCAAGCTCCAAAATTATTAGCTGATAAATGA", "aa": "MQTPTNYSLLTQEVNENANKVKKALGALDLCFELNDPSNILTNQDQFSIKYAMLNNLKQSVSATGQVEIKENKKIVKFHISGIDLNQDYKVCELMLLTKPARMGEKINNNDKNNYSFIYQNSTNDDNQYKFKILLENNDLSTKERPSKTGDIVNNKGKVDITITQAPKLLADK", "aa_hexdigest": "045f9bfcfb04b0ddd95927899f38c05f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19621.047800000004, "isoelectric_point": 8.391349983215335 }, "id": "NKIECMJPJD_578", "locus": "GCA000828735_00563" }, { "type": "cds", "sequence": "contig_1", "start": 325837, "stop": 326208, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGACTACAAATCGTTATTATAATTTAAATGGTTTATATTTTGCAAATCAACAAAGTGATTTTGAACAAGGTAATGCTACATATTTACTAAGTTTATACCCAGCAAATTTATTAGTGCCAACTGATGGTGTACGAATTAAAAATATTACAACGCCAGCCACAACTGATGCTGGATCAATTTCATTTAAATTAGAATCAAAAGATCAAGTATTTGACAAACAAGTATTACATATTCAAATTACACCATCTAATCAATATATTCCAGTATTTGAACGCACATACACATTAAGTGAAAATTCTAATAGTGCAAATGTATTTTTAACTTTACACAATTTAGAGCCTAATACTGAATATAAAATTACTAAAATCTGA", "aa": "MTTNRYYNLNGLYFANQQSDFEQGNATYLLSLYPANLLVPTDGVRIKNITTPATTDAGSISFKLESKDQVFDKQVLHIQITPSNQYIPVFERTYTLSENSNSANVFLTLHNLEPNTEYKITKI", "aa_hexdigest": "90c654fea1e5f0c9ae50dfd36462ddec", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13987.478099999986, "isoelectric_point": 5.562343406677246 }, "id": "NKIECMJPJD_579", "locus": "GCA000828735_00564" }, { "type": "cds", "sequence": "contig_1", "start": 327082, "stop": 327330, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATGAAAATCCACAAACAAAAATGTATAAAGATAAAGTAACTTTTGAATTAGATAATAGTAAATATGCAAGTATTAAAATTGAAGAAAACCAAAATCCTTATGAAGTGAAAAAAGTGAAAAATAAATTAGATAATGAAAATTTAACACTAAAATCAACTGTGATATTTAACAATAAAAATGAACATCTTTATAATAAATACATGTTTGCTTTATTTACTAATACTAAAACAAATGCAGAAGTTTGA", "aa": "MYENPQTKMYKDKVTFELDNSKYASIKIEENQNPYEVKKVKNKLDNENLTLKSTVIFNNKNEHLYNKYMFALFTNTKTNAEV", "aa_hexdigest": "de1ace2ccf8ea8ac8e0891c221a11fef", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9790.979999999996, "isoelectric_point": 8.753597831726076 }, "id": "NKIECMJPJD_580", "locus": "GCA000828735_00565" }, { "type": "cds", "sequence": "contig_1", "start": 327931, "stop": 328101, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGGAGCTCAAATTGCATTTGATCAAACTACAGTTGTTAAAAATGATATTGTCTTTACAAAAGGTAGTTCTGGTGGTTACCCAGCATCAGTGATTAATTTTGAATTAAAAGGTAAAGAAGCAAATACTTTTGTTGAAGGACAAACTTATAATGTAGTTTTATCAACATGA", "aa": "MGAQIAFDQTTVVKNDIVFTKGSSGGYPASVINFELKGKEANTFVEGQTYNVVLST", "aa_hexdigest": "1f28c57dc43b9670c03762c982e8c13c", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5973.629100000002, "isoelectric_point": 4.93575038909912 }, "id": "NKIECMJPJD_581", "locus": "GCA000828735_00566" }, { "type": "cds", "sequence": "contig_1", "start": 328177, "stop": 328353, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATTGTTTCAATTGTTGCGCATAATAATCGTTTAGAATTTAAAAATATTCAATTAATTCCAAACCAAAATTATAATATAGTTAAAGTCTTACACTATCAAGCATCAAATACAATTTCAGCTAAAGATATGGGATATAGTTTAAAAAATGCATTTAGTACCAAATCAGTTGATTGA", "aa": "MIVSIVAHNNRLEFKNIQLIPNQNYNIVKVLHYQASNTISAKDMGYSLKNAFSTKSVD", "aa_hexdigest": "4dd300b03615c9fc73e2dc4e1cfd4bcb", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6582.478200000003, "isoelectric_point": 9.522963523864746 }, "id": "NKIECMJPJD_582", "locus": "GCA000828735_00567" }, { "type": "cds", "sequence": "contig_1", "start": 329132, "stop": 330310, "strand": "-", "frame": 2, "gene": null, "product": "toprim domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002100652A", "UniRef:UniRef90_UPI002100652A" ], "nt": "TTGGTAATTGATAAGAATGGTAATTACCATGATTTTTTTAATGATCGTATTATTTTTAGTATTTATGATCATCTCAATAACGTTGTTGGTTTTAGCGGACGTATAATTACAAATGAGAAAACACCAAAATATTTAAATACTAAAACCACTTCTGTATTTAAAAAAGATGAAGTTCTTTATAATTTTCATAATGTTATCACCATTGAAAATAATCATCAATTATTTATAGTTGAAGGTTTTATGGATGTCATTACAATTCATAAATCTAACAAACCAAACGTTGTAGCAACAATGGGTGTTGAATTAACTAATAAACATTTAGAATTAATTAAAAGCTATGGAATTAAAGATTTAATTTTATGTTTTGATAACGATCAAGCAGGTTTTTTAGCTACTAAAAAACAAATTATGAAATTAATTAATTCCCCATTCAACATTTTTATTGTTAATCATAAAGATAATGATTGTAAAGACATGGATGAATATGCAAATAAGCACGGTTATAATGCAACAAGTAAGTTATTAGACCAACAACTTCATATTAGTGAATTTTTATTATTAGAAATTAGTAATACAAACAAATCTACCCCCTCACTAAAACACAGTTATAAACAAGAGTGTTTTACAATCATTAAAAAATATGGTGATATTTTTTATGTAGATAAATATGTTGATTTCATGAAACAAACATTTTCAATTGAAGAAAATTTATTAAAAAAAGTTATTGAAGAATTATTAATTAACAAACAATGCAATTCTATTCAAAACAAAAAGAACATTCAATCATATCAAAAACAAACACAAGTAACTAATAAACTTACTACTTCAAACCCAAAAGTTATCCTAACAAAAATTGATAAACATTTTAACCACTTAGTAGTAATTATATTAATGAATCGGGTTTTCTTAAACCGTCTTGATACGCAAGTTCGTAACCGGTTAACTAAAGAGCAAAGTTTAGTGATTAATATTATAGAAGACTTTTATTTGTACCATTTTAAGATTAATAATATTTTAAAAAACACTGATTCTTTTATTGAATTTATTAATAAAAACTACAGTAAATATACTAATGTTATTAACCAACTTACAAAAATGGTGCAGATTATTAAATATAACCCTAAGTTTGATAATATAGTAGCATTTGAACTAGCTAAAAATAATTTTATATATGAATGA", "aa": "MVIDKNGNYHDFFNDRIIFSIYDHLNNVVGFSGRIITNEKTPKYLNTKTTSVFKKDEVLYNFHNVITIENNHQLFIVEGFMDVITIHKSNKPNVVATMGVELTNKHLELIKSYGIKDLILCFDNDQAGFLATKKQIMKLINSPFNIFIVNHKDNDCKDMDEYANKHGYNATSKLLDQQLHISEFLLLEISNTNKSTPSLKHSYKQECFTIIKKYGDIFYVDKYVDFMKQTFSIEENLLKKVIEELLINKQCNSIQNKKNIQSYQKQTQVTNKLTTSNPKVILTKIDKHFNHLVVIILMNRVFLNRLDTQVRNRLTKEQSLVINIIEDFYLYHFKINNILKNTDSFIEFINKNYSKYTNVINQLTKMVQIIKYNPKFDNIVAFELAKNNFIYE", "aa_hexdigest": "28afb998837badab902c2cb57c0bef35", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002100652A", "query_cov": 0.8086734693877551, "subject_cov": 0.8521505376344086, "identity": 0.943, "score": 591.0, "evalue": 1.05e-210, "valid": true, "product": "toprim domain-containing protein", "uniref50_id": "UniRef50_UPI002100652A" }, "pscc": { "uniref50_id": "UniRef50_UPI002100652A", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002100652A" ], "product": "toprim domain-containing protein" }, "genes": [], "id": "NKIECMJPJD_583", "locus": "GCA000828735_00568" }, { "type": "cds", "sequence": "contig_1", "start": 330518, "stop": 330688, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATTAAATTTGTTGCTGAATATAAAAAGATCTCTTATACTTTAGCAGCACAAGAGATTGTTCAATTAATGAATTTAGATTTAAATTTATTGAATCATTTACAAAAAATTTCGCCACAAGAAATTAAAAAAAATAAGGTTTTTGATTTAAACAAACAAATTCAAAAATGA", "aa": "MIKFVAEYKKISYTLAAQEIVQLMNLDLNLLNHLQKISPQEIKKNKVFDLNKQIQK", "aa_hexdigest": "3508f9785f943f742700fb1b584eee54", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6615.803100000001, "isoelectric_point": 9.574602699279783 }, "id": "NKIECMJPJD_584", "locus": "GCA000828735_00569" }, { "type": "cds", "sequence": "contig_1", "start": 330785, "stop": 330892, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAAAAATAACTAATAATGTTTTTTTAACAATTCGTAATGCTGTAAATATTTCTGATGTTATTCAAAATTACATTAAAGTAATTAAAAAGGGTAATAATTATTGA", "aa": "MSKITNNVFLTIRNAVNISDVIQNYIKVIKKGNNY", "aa_hexdigest": "290b1b5f3c0ca77ac7741defab21b7c7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4026.6600000000017, "isoelectric_point": 9.997450828552246 }, "id": "NKIECMJPJD_585", "locus": "GCA000828735_00570" }, { "type": "cds", "sequence": "contig_1", "start": 330880, "stop": 331389, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03129.25" ], "nt": "ATGAATTTATCAAAAAAAGATTTAACATATTTTGATGAAGAAACTAAGCAAAAATATATTCCTCATGTTATTGAACCATCAGTCGGTGTAGAAAGATTATTATATGCTATTTTGACTGAGGCTACATTTATTGAAAAACTAGCAAATGATGAAGAACGAATTTTAATGAATTTAAAATATGATCTAGCCCCTTATAAAATTGCTGTAATGCCGTTAGTTAATAAGTTAAAAGAAAAAGCAGAAGCAGTTTATAATAAAATTTTGGATTTAAATGTTTCAGTAACTTTTGATAATAGCGGAAGTATAGGAAAACGTTACCGCCGTCAAGATGCTATAGGAACAATTTATTGTATAACTATTGATTATGATTCATTTGGTGATGAACATGACCCTACTTTTACTATTCGTGAACGAAACACAATGGCTCAAAAAAGAATTAAATTATCAGAATTATCTTTATATTTAAGCCAAAAAGCTCATGAAAACTTTCAAAAGCAATGTCAAAAATAA", "aa": "MNLSKKDLTYFDEETKQKYIPHVIEPSVGVERLLYAILTEATFIEKLANDEERILMNLKYDLAPYKIAVMPLVNKLKEKAEAVYNKILDLNVSVTFDNSGSIGKRYRRQDAIGTIYCITIDYDSFGDEHDPTFTIRERNTMAQKRIKLSELSLYLSQKAHENFQKQCQK", "aa_hexdigest": "fc235b1c64efcf97487a45cab37f4e86", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03129.25", "name": "HGTP_anticodon", "length": 96, "aa_cov": 0.5443786982248521, "hmm_cov": 0.96875, "evalue": 4.9124622679641325e-18, "score": 60.70277786254883, "start": 66, "stop": 157 } ], "seq_stats": { "molecular_weight": 19678.35749999999, "isoelectric_point": 6.838435554504395 }, "id": "NKIECMJPJD_586", "locus": "GCA000828735_00571" }, { "type": "cds", "sequence": "contig_1", "start": 331561, "stop": 331848, "strand": "-", "frame": 3, "gene": null, "product": "Aminoacyl-transfer RNA synthetases class-II family profile domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_X1KVU4" ], "nt": "ATGTTTAAAACACACCAAGGAGTAATTGAAGATCCTAAAAACATCGTTTATTTAAGACCAGAAACCGCTCAAGGTATTTTTGTTAATTTCAAAAATGTTCAGCGATCAATGCGTTTACATTTACCATTTGGGATTGCACAAATAGGTAAATCATTTAGAAACGAAATAACTCCTGGTAATTTCATTTTTAGAACTCGTGAATTTGAACAAATGGAAATTGAATTTTTCTTAAAAGAAGAAACTGCATATGATGTTTTTGATAAATATTTAAACCAAATTCAAAATTGA", "aa": "MFKTHQGVIEDPKNIVYLRPETAQGIFVNFKNVQRSMRLHLPFGIAQIGKSFRNEITPGNFIFRTREFEQMEIEFFLKEETAYDVFDKYLNQIQN", "aa_hexdigest": "f19a65ba8d2743799bfd7c1be8c4d3e6", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI003241E146", "query_cov": 0.8210526315789474, "subject_cov": 0.8478260869565217, "identity": 0.795, "score": 125.0, "evalue": 6.79e-36, "valid": false, "product": "aminoacyl--tRNA ligase-related protein", "uniref50_id": "UniRef50_X1KVU4" }, "pscc": { "uniref50_id": "UniRef50_X1KVU4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_X1KVU4" ], "product": "Aminoacyl-transfer RNA synthetases class-II family profile domain-containing protein" }, "genes": [], "id": "NKIECMJPJD_587", "locus": "GCA000828735_00572" }, { "type": "cds", "sequence": "contig_1", "start": 332197, "stop": 332301, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGATATAAATTTAAAACACAAGAAGAATTAGTAAATCATTTAAAATCTGTTGGTTTTGTTTTTGCGAATTCAGAAATATATAATGGGTTAGCTAACGCATGA", "aa": "MRYKFKTQEELVNHLKSVGFVFANSEIYNGLANA", "aa_hexdigest": "34d15d749e3e91c54b3d938d77ee70e4", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3919.4208000000017, "isoelectric_point": 8.161520195007324 }, "id": "NKIECMJPJD_588", "locus": "GCA000828735_00573" }, { "type": "cds", "sequence": "contig_1", "start": 332478, "stop": 333053, "strand": "-", "frame": 1, "gene": "recO", "product": "DNA repair protein RecO", "db_xrefs": [ "COG:COG1381", "COG:L", "GO:0003677", "GO:0006281", "GO:0006310", "KEGG:K03584", "SO:0001217", "UniRef:UniRef50_A0A084F1G9", "UniRef:UniRef90_A0A2C9DYJ4" ], "nt": "ATGGCTGAAATAATAACTAGAGGATATTTAGTTGCACGCCAAGATTATGATATTTTTGATGAAATATTAACATTCATTAATGAATATGGCAATCGTTTTATTATGTTTGCACCTGGAACAAAAAGAATTACTTCTAAAAACGCAAGAGCTTTGTTTTTTGGTAATTTTTTAGAAATACAATTTTTTTATGCAAGAAATGATACAAAACTTTCAAAATTAAAAAAAGTTATTTCCTTAGATCAAATTAATTATAAGTACGAAAATACATATTCAATGCTAATATTAAGCGAATTAATGACTAAAGTTGTTAGTTTTAATGTTGAATTCTACCAATTCTATCAACTAATACTAGAATATATCATTTTAGAATATAATGATTATTATATAAGTTGTTTTTTATTGGTAAAATTTTTAATAATGAATGGAGTTAATTTTAATTTTCAAGCATGTGTTCATTGTAATAGCTCAAAAAATATTAAAACTTTTTCTTTGGCTGATCATGGTCTTATATGTGTTAACTGTGAGTCTAAGATTGTTAACAAAATAGATTATGAACCAAAGGCGTTAAAATTGTGA", "aa": "MAEIITRGYLVARQDYDIFDEILTFINEYGNRFIMFAPGTKRITSKNARALFFGNFLEIQFFYARNDTKLSKLKKVISLDQINYKYENTYSMLILSELMTKVVSFNVEFYQFYQLILEYIILEYNDYYISCFLLVKFLIMNGVNFNFQACVHCNSSKNIKTFSLADHGLICVNCESKIVNKIDYEPKALKL", "aa_hexdigest": "d4d3c46e96651fcfec372b6df69f40aa", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A2C9DYJ4", "query_cov": 1.0, "subject_cov": 0.8025210084033614, "identity": 0.995, "score": 376.0, "evalue": 2.67e-131, "valid": true, "gene": "recO", "product": "DNA repair protein RecO", "uniref50_id": "UniRef50_A0A084F1G9", "cog_id": "COG1381", "cog_category": "L", "kegg_orthology_id": "K03584", "go_ids": [ "GO:0003677", "GO:0006281", "GO:0006310" ] }, "pscc": { "uniref50_id": "UniRef50_A0A084F1G9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A084F1G9" ], "product": "DNA repair protein RecO" }, "genes": [ "recO" ], "id": "NKIECMJPJD_589", "locus": "GCA000828735_00574" }, { "type": "cds", "sequence": "contig_1", "start": 333104, "stop": 333955, "strand": "-", "frame": 2, "gene": "era", "product": "GTPase Era", "db_xrefs": [ "COG:COG1159", "COG:J", "GO:0000028", "GO:0003723", "GO:0003924", "GO:0005525", "GO:0005829", "GO:0005886", "GO:0043024", "GO:0070181", "SO:0001217", "UniRef:UniRef50_Q9PPZ9", "UniRef:UniRef90_Q9PPZ9" ], "nt": "ATGATTAAAAAATACGGAATCGTAGCTATTGTTGGAAAACCAAATGTTGGTAAATCAACATTAATTAATGCAATTATGAAAAAAAAAGTCTCAATCATTTCAAATAAACCACAAACAACACGCAATGCTGTTAAAGAGATTTATGAAGATGATGAATCAGCAATTATTTTTACTGATACACCGGGGTTTCATGAACCATCAAATAAACTAGATTTATTTTTAAATCATGAAATTGAAATTTCATATAAAGAAGCAAATATAATCTTATTCGTGACAACTATGGACAAGGAATTAGATGCCAATGATTTTGAAATTATTAATTTAATTAAAGAAGCAAACAAAGAAAATGTTATTTTAGTCATTTCAAAAGCTGAAATGGCTAAGAATCAGGATCAAATTGATGAGAGAATCCACTTTTTAAAAAAACATCTAGTATTTAAAGATGTTGTTCAGATAAGTGCTTTACATGCTATTAACATTGATAAATTAATTAGTACAATCAAAAATTACCTTCACAAAGATGTGGTAACAGATTATTTTCGTCAAAAAGCCGAAAAAGAAGATAAATTTGTTATTACTGAAATTATTCGTGAACAATGCTTATTAAATTTAAACCATGAAGTTCCTCACGGTGTTGGTGTAGAAATTGATGAAAGTAAATACAACCAAGAAGCAAATCATTGGATAATTAAAGCTTCAATAATAATTGAAAAAAATTCTCATAAACCTATTATTATTGGCCAAAATGGTACGATGATTAAAAAAATTAGCATGGGAGCAAGAAAACAGTTGCATGAAATTTATGATTGTCATATTTCATTAACAATTTTTGTTAAAGTTGAAAACAATTGA", "aa": "MIKKYGIVAIVGKPNVGKSTLINAIMKKKVSIISNKPQTTRNAVKEIYEDDESAIIFTDTPGFHEPSNKLDLFLNHEIEISYKEANIILFVTTMDKELDANDFEIINLIKEANKENVILVISKAEMAKNQDQIDERIHFLKKHLVFKDVVQISALHAINIDKLISTIKNYLHKDVVTDYFRQKAEKEDKFVITEIIREQCLLNLNHEVPHGVGVEIDESKYNQEANHWIIKASIIIEKNSHKPIIIGQNGTMIKKISMGARKQLHEIYDCHISLTIFVKVENN", "aa_hexdigest": "ec7ac9b69363bf2046e4efcb52064860", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_Q9PPZ9", "query_cov": 1.0, "subject_cov": 0.9433333333333334, "identity": 0.9790000000000001, "score": 544.0, "evalue": 5.33e-195, "valid": true, "gene": "era", "product": "GTPase Era", "uniref50_id": "UniRef50_Q9PPZ9", "cog_id": "COG1159", "cog_category": "J", "go_ids": [ "GO:0000028", "GO:0003723", "GO:0003924", "GO:0005525", "GO:0005829", "GO:0005886", "GO:0043024", "GO:0070181" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PPZ9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PPZ9" ], "product": "GTPase Era" }, "genes": [ "era" ], "id": "NKIECMJPJD_590", "locus": "GCA000828735_00575" }, { "type": "cds", "sequence": "contig_1", "start": 333948, "stop": 334415, "strand": "-", "frame": 1, "gene": "ybeY", "product": "rRNA maturation RNase YbeY", "db_xrefs": [ "COG:COG0319", "COG:J", "EC:3.1.-.-", "GO:0004222", "GO:0004521", "GO:0004540", "GO:0005737", "GO:0006364", "GO:0008270", "GO:0042254", "GO:0046872", "RefSeq:WP_006689014.1", "SO:0001217", "UniParc:UPI00016C0F41", "UniRef:UniRef100_UPI00016C0F41", "UniRef:UniRef50_Q9PQ00", "UniRef:UniRef90_Q9PQ00" ], "nt": "ATGCATTTTTTAATAACGAATGAAGTTAAAAAAATCTTTAATCATAAATTATATTCACAACGTTTTAAACAAATAACCGATTTGGTTTCCACGAAATTAAATATTGATAAAAAGCGATTTTTTGAATGTCATTTTGTTGATGAAAAAACGATACAAGAAATTAATAGAAACTATCGTAATAAAGACTATATTACAGATGTTATTAGTTTTGCATTTGATGATGGTGAAATTATTACTCCTTTGTTAGGAGAAATGTATATTTGCTATCAAAAAGTTGTTGATCAAGCAAATCAATTTGCTCATTCATTTGAACGTGAGCTATGCTTTTTATTTACACACGGCCTACTTCATTTATTAGGTTATGATCATATGAAAATTGAAGATGAAAAAATCATGTTTGCTTTACAAGACGAAATTTTAAATGAATTAAAAATTACAAGAAATATAAATGGTGCTAAAAATGATTAA", "aa": "MHFLITNEVKKIFNHKLYSQRFKQITDLVSTKLNIDKKRFFECHFVDEKTIQEINRNYRNKDYITDVISFAFDDGEIITPLLGEMYICYQKVVDQANQFAHSFERELCFLFTHGLLHLLGYDHMKIEDEKIMFALQDEILNELKITRNINGAKND", "aa_hexdigest": "bf4fba258a9d67556f7118ffdf0b1072", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI00016C0F41", "ncbi_nrp_id": "WP_006689014.1", "uniref100_id": "UniRef100_UPI00016C0F41", "db_xrefs": [ "SO:0001217", "UniParc:UPI00016C0F41", "RefSeq:WP_006689014.1", "UniRef:UniRef100_UPI00016C0F41" ] }, "ips": { "uniref100_id": "UniRef100_UPI00016C0F41", "uniref90_id": "UniRef90_Q9PQ00" }, "psc": { "uniref90_id": "UniRef90_Q9PQ00", "gene": "ybeY", "product": "rRNA maturation RNase YbeY", "ec_ids": [ "3.1.-.-" ], "uniref50_id": "UniRef50_Q9PQ00", "cog_id": "COG0319", "cog_category": "J", "go_ids": [ "GO:0004222", "GO:0004521", "GO:0004540", "GO:0005737", "GO:0006364", "GO:0008270", "GO:0042254", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQ00", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQ00" ], "product": "Endoribonuclease YbeY" }, "genes": [ "ybeY" ], "id": "NKIECMJPJD_591", "locus": "GCA000828735_00576" }, { "type": "cds", "sequence": "contig_1", "start": 334920, "stop": 335528, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATGATTTTCAAACAGAAGCAGAACAGTTAAATTTATTAAAAACTACAGCGAATCTAAATCAAAAACATTTACAAGCATTAGGTGTAAATAATGTTAGTGCTAAATATGCTATTTATGAACGTATTAATAAGAATACAAAAAAACACGAAAAATTTGGGGAAATTGTTTATGAATTTTATCCCACAAATTCAAAATTAGATATCGTTAATTTTCTAACTAAAACAAAACCATACGAAAAAATTAATTCAAAAATCCAACTTTTAAGCTTATTTACTACGGCTAACCGTTTAGAACTACAAAACATTTCTTCACTTTTTTCACCAAAACCTGAACTTTCACAAAACAGGGATTTTGATAATACAAATTGGTTAACTTTAAATAATGATCCTAAAAAAATCAGTGTTAAAACGACTAATGATGGTCAACAAGTTGAAATTTTATTACCAAAAACTAGTTTTGATCATCAAAAATTTAATTTAGATTTTTTTGCTAAAGAGTTTGATGCTAATTTTAATTTTAATCCAATGGGTAATAAAACAAGAGCTCAAGCTGTAGCGACTTTTGATAGAACAAAACAAAAACGAAAAGCTCCTGTTAATTCTTGA", "aa": "MNDFQTEAEQLNLLKTTANLNQKHLQALGVNNVSAKYAIYERINKNTKKHEKFGEIVYEFYPTNSKLDIVNFLTKTKPYEKINSKIQLLSLFTTANRLELQNISSLFSPKPELSQNRDFDNTNWLTLNNDPKKISVKTTNDGQQVEILLPKTSFDHQKFNLDFFAKEFDANFNFNPMGNKTRAQAVATFDRTKQKRKAPVNS", "aa_hexdigest": "a8c91cff0aebc749b4f197cba2e5ff9b", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 23357.125199999995, "isoelectric_point": 9.521545219421387 }, "id": "NKIECMJPJD_592", "locus": "GCA000828735_00577" }, { "type": "cds", "sequence": "contig_1", "start": 336240, "stop": 336437, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F37C014" ], "nt": "ATGGATGCGGTTTTGATTGGAATTGCAGTTTTAATCTTAATCATTGCCATTATTATTAGTCTTTTATATAAAATTCCAGGTTTAATTTATAGTTTATTAATGGCTTTTAGTTTTGTTATTAGTTTGTTACTAATAAAATCAAGTAATTTAGGGTTTAGTACAGAAACTTATAGTGCATTAATTATTGGTATTTGTTGA", "aa": "MDAVLIGIAVLILIIAIIISLLYKIPGLIYSLLMAFSFVISLLLIKSSNLGFSTETYSALIIGIC", "aa_hexdigest": "946c04a5884aff0241818de08d23ce87", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001F37C014", "query_cov": 1.0307692307692307, "subject_cov": 1.0, "identity": 0.8809999999999999, "score": 89.7, "evalue": 2.14e-22, "valid": false, "uniref50_id": "UniRef50_UPI001F37C014" }, "pscc": { "uniref50_id": "UniRef50_UPI001F37C014", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F37C014" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6974.581699999996, "isoelectric_point": 5.819937705993653 }, "id": "NKIECMJPJD_593", "locus": "GCA000828735_00578" }, { "type": "cds", "sequence": "contig_1", "start": 336843, "stop": 337223, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATCAAAAGGTTTATAGAATTAGCTTTTTAATTTTTATCTTTATTTTAGGTTTTATTGGATTATTTATTTTAGGTTTTATTGTTCCAAACCATAGTTTTAGTTTTGGTTCTGTATATGAGTTAATTATGAAAAGTGACTTAAAAGACTTTAATTCTCATGAGTTCAAAACCATTTTAGATTATAAAATTAATGATGGAGTAGTATCAATGCAATTTGATGCTAAAAAACCAAATGTTTTAAGTGAAATAGATTTGATCAGTTTAAAGCACCGCTTTAGTGATGCAATTGTTTATAGAAGTAGTAAATATCATTTAATTAATAATATTACAAATTCAATTGCTGCGTATTTTATTATCTTTGCTATTATTTTAATTTGA", "aa": "MNQKVYRISFLIFIFILGFIGLFILGFIVPNHSFSFGSVYELIMKSDLKDFNSHEFKTILDYKINDGVVSMQFDAKKPNVLSEIDLISLKHRFSDAIVYRSSKYHLINNITNSIAAYFIIFAIILI", "aa_hexdigest": "368e3b1e9df6f4f5ac533e162d3da6b5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14580.980599999983, "isoelectric_point": 8.861646842956542 }, "id": "NKIECMJPJD_594", "locus": "GCA000828735_00579" }, { "type": "cds", "sequence": "contig_1", "start": 337371, "stop": 337535, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACTAGTTTTAGTTTACATATTTGTTTGAATTTAAAACAAACTCTGGATTTAATGAAGCATGTAACAAAAAAACAATTACAATTTCAAATTCAAGATTCTTTAATTAAATACTTTAATACTTATAATATAATATACGTTGTGATGTTATTTGCATTATTATGA", "aa": "MTSFSLHICLNLKQTLDLMKHVTKKQLQFQIQDSLIKYFNTYNIIYVVMLFALL", "aa_hexdigest": "ad473f0ad218d6fe83b917e3cb9f0300", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6452.712099999999, "isoelectric_point": 9.302932929992675 }, "id": "NKIECMJPJD_595", "locus": "GCA000828735_00580" }, { "type": "cds", "sequence": "contig_1", "start": 337641, "stop": 337748, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAATGGTTTATATTAGTAAAGGTTTATTAGCTAAGAATGATAATGCAAATAATGTTTATGATAAATTTGATGAACAAGAAATTATGAATATAAATAAATTTTAA", "aa": "MKMVYISKGLLAKNDNANNVYDKFDEQEIMNINKF", "aa_hexdigest": "8f8ce4b0e12e51ca8240cac9cfe8fdeb", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4139.7282, "isoelectric_point": 6.000117301940917 }, "id": "NKIECMJPJD_596", "locus": "GCA000828735_00581" }, { "type": "cds", "sequence": "contig_1", "start": 337769, "stop": 338290, "strand": "+", "frame": 2, "gene": "apt", "product": "adenine phosphoribosyltransferase", "db_xrefs": [ "COG:COG0503", "COG:F", "EC:2.4.2.7", "GO:0002055", "GO:0003999", "GO:0005737", "GO:0006166", "GO:0006168", "GO:0009116", "GO:0016208", "GO:0044209", "KEGG:K00759", "RefSeq:WP_004026209.1", "SO:0001217", "UniParc:UPI000169D16F", "UniRef:UniRef100_A0AAP9D7M0", "UniRef:UniRef50_Q9PQ02", "UniRef:UniRef90_Q9PQ02" ], "nt": "ATGATCAATATTGATTACATTAAATCAAAAATCAGGGATGTACCAGACTTCCCTAAAAAAGGAATCGTTTTTAAGGATATTACTCCTTTATTTTTAGAACCAAAAATTATTGAAAAGATTGTTGATGATTTTGCTGACTTTGCTAAATCATTAAATATAGATGCTATAATAGGTGCAGAGTCGCGTGGTTTTTTGTTTGCAGCACCATTATCAATCAAATTAAATAAGCCATTTATTTTGGTACGTAAACCTAATAAGTTACCAAATGATGTTTATAGTGCTGAATATACATTAGAATATGGTAGTTCTCGTGTGGAGATGCACAAAGATGCACTAAAACCAAACCAGCGCGTTTTAATTGTCGATGATTTATTAGCAACAGGAGGGACTGTTGCTGCCATTGAAAATCTTGTTCATCAAGCTAAGGGAATTGTAGCAGGGAGCGTTTATTTAATTCGCTTAGGATTTCTAAAAGGTGAAGAAAAACTAAGTGGAAAAGTTCATGCTTTAATTAATTATTAA", "aa": "MINIDYIKSKIRDVPDFPKKGIVFKDITPLFLEPKIIEKIVDDFADFAKSLNIDAIIGAESRGFLFAAPLSIKLNKPFILVRKPNKLPNDVYSAEYTLEYGSSRVEMHKDALKPNQRVLIVDDLLATGGTVAAIENLVHQAKGIVAGSVYLIRLGFLKGEEKLSGKVHALINY", "aa_hexdigest": "fba6a11753cdd6b8c54aef04230f8352", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000169D16F", "ncbi_nrp_id": "WP_004026209.1", "uniref100_id": "UniRef100_A0AAP9D7M0", "db_xrefs": [ "SO:0001217", "UniParc:UPI000169D16F", "RefSeq:WP_004026209.1", "UniRef:UniRef100_A0AAP9D7M0" ] }, "ips": { "uniref100_id": "UniRef100_A0AAP9D7M0", "uniref90_id": "UniRef90_Q9PQ02" }, "psc": { "uniref90_id": "UniRef90_Q9PQ02", "gene": "apt", "product": "adenine phosphoribosyltransferase", "ec_ids": [ "2.4.2.7" ], "uniref50_id": "UniRef50_Q9PQ02", "cog_id": "COG0503", "cog_category": "F", "kegg_orthology_id": "K00759", "go_ids": [ "GO:0002055", "GO:0003999", "GO:0005737", "GO:0006166", "GO:0006168", "GO:0009116", "GO:0016208", "GO:0044209" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQ02", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQ02" ], "product": "Adenine phosphoribosyltransferase" }, "genes": [ "apt" ], "id": "NKIECMJPJD_597", "locus": "GCA000828735_00582" }, { "type": "cds", "sequence": "contig_1", "start": 338884, "stop": 339354, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATTCAAACTAATAAAATGTTAACTAAATCCATTAATTTTAATAAAGAAGATCATTTAATTAAAGTCGTTGATCACAGCGCACAATTTACTAAAATTAAAAAAACAGAAGTTGAATTTGAAATTAAGCTTTTAGATCTAAATCTTAATGATATTAAAAATAAAAATATTGAAATAGAATTAACAAATAAAAAAACAAATAAAACCTTTAAATTGCATTTTCTAAAATATCAATTTAATCACTTAACACAACAAAATAAAAAACCAGTATTAACTATTACTGCTAATTTAAATGCTGATGCAAATAACCAATTAGATGATGGCGAATATTTTATTTCTAAATTAGTTTTAGACCAAAAAAAACTTGAAATTAACCAAAGCGTTTATAAACCAAGTGGGTTACAGTTAGATGGAAGTATTAATAATCAAAGACCAACTTATTTATTAATTCCATCTGTTTTTTCAAAATGA", "aa": "MIQTNKMLTKSINFNKEDHLIKVVDHSAQFTKIKKTEVEFEIKLLDLNLNDIKNKNIEIELTNKKTNKTFKLHFLKYQFNHLTQQNKKPVLTITANLNADANNQLDDGEYFISKLVLDQKKLEINQSVYKPSGLQLDGSINNQRPTYLLIPSVFSK", "aa_hexdigest": "44153f8cb2737ce58f9d731f20ecdd8c", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18119.666799999977, "isoelectric_point": 9.377136039733887 }, "id": "NKIECMJPJD_598", "locus": "GCA000828735_00583" }, { "type": "cds", "sequence": "contig_1", "start": 339382, "stop": 340452, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATTAAAAATGATGGAATTCCACAACAATTACAACTTTCATATGATCATTTTATTAACCCATATAATTTTAAAGATAATATGTTAAGTGCAGATATTGAACTTATATCAACTAAAAATAATAAAACTATTATTTTAAAATCATTAAAGTTTAATCACGATAAAAACAATATTGTTTTTGATTTAGTCAATAATTTAGAATTACAAACTCTTATTTTAAAAACTCCTTTTTTTAAAATTAAATCATTAACAATAATGAATGATAAGCAAACTTTGCCATTAAGTATTAGAGAAAAACAGCTTGATTTAACTAATAAACATCTTTCTAAAATCATTAAATTTACATTTGATGATACTGATCCACAAAATATGCTTATGAAATTTCAATTAGATAGTGATTCGCCAATAGTGCAAAATACTAAAAAAGTTTTATTAACAATTAAAAAAATAAATACAGATCAGCAAGAAGATATAAAGACCTTTAGTTTTGATATTTTAGAAAACAAAATAACAACAGGTTCTTTTTATGATGTAAAAAATAAGTATTTTCTATTAAATCTAAATAAAACTTTGAATGCTAAATCGATAGGTCAAATTCTTGAAAATGATTCTAATTATATAATTACTAAATTAACTGTGGTTAATAATGAAGCATCAATCAATGAAATACCAATTGAAATTGATGATTTACAAAATAAACAATTTACGTTAAAATTTGCTGAATACCAATCAAATGTAATTAAATTTAATGCTAAGAATGATCCTGAATTACATCTTAATATCAAACATTTTAAACAACTTAATCTTACAAAAATTAATAAAATTAAATTGACTTTAAAAGACGGTAAGCAAATTATTTTTAATCGTTTAAATAATGAATTTAGTTTCCAACAAGCAGGATTTAAATTAAAAATTAACCAAAACACATTTCCAAATTATTTAAAACAACATCAATATGTAATTAAAAGTATTAGTTTAATAAACGATGATCAGTCTGTGATTGAGTTATTATCAAAACACAATCATGAAGCTATTTTTGAGATTCCTATTACAGCATCTCTTGATACTTGA", "aa": "MIKNDGIPQQLQLSYDHFINPYNFKDNMLSADIELISTKNNKTIILKSLKFNHDKNNIVFDLVNNLELQTLILKTPFFKIKSLTIMNDKQTLPLSIREKQLDLTNKHLSKIIKFTFDDTDPQNMLMKFQLDSDSPIVQNTKKVLLTIKKINTDQQEDIKTFSFDILENKITTGSFYDVKNKYFLLNLNKTLNAKSIGQILENDSNYIITKLTVVNNEASINEIPIEIDDLQNKQFTLKFAEYQSNVIKFNAKNDPELHLNIKHFKQLNLTKINKIKLTLKDGKQIIFNRLNNEFSFQQAGFKLKINQNTFPNYLKQHQYVIKSISLINDDQSVIELLSKHNHEAIFEIPITASLDT", "aa_hexdigest": "5ffdff880481187b0f375e9de5af31ea", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 41414.2008, "isoelectric_point": 8.93223972320557 }, "id": "NKIECMJPJD_599", "locus": "GCA000828735_00584" }, { "type": "cds", "sequence": "contig_1", "start": 340489, "stop": 340869, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATTAGATAGTTTAAGTTTACATCAAAGCTTAAATCCATCAGATTATGCTTTAAATTTTTATAATCTTGATACTAATATTATTAACCGAAAATTTACACTTAGTCCCCAAACTGTTTTATCTTCAAAAGTTAATAATTCAAATGTAATTGAATTAATTTATGATTTAGATAATGATGAAAATATTTATGGAGGTTTAGAACCTGAGAGCACTTATCAAATTAGTTCGTTAATGATAAATGCTAAACCAGTGATTATTAATACTTCTATAACAATGGTTCAAACTAACGCTCCAGCTTCTAAGGTAGAAAAAATTGTTGCTAGTTATGATTCAGTAACCAAAAAAACAAAAATATCATTGCAGTTTTCTAATAATTGA", "aa": "MKLDSLSLHQSLNPSDYALNFYNLDTNIINRKFTLSPQTVLSSKVNNSNVIELIYDLDNDENIYGGLEPESTYQISSLMINAKPVIINTSITMVQTNAPASKVEKIVASYDSVTKKTKISLQFSNN", "aa_hexdigest": "ad3a544fec90273be94812ba97a39efd", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14049.711699999983, "isoelectric_point": 5.229266929626463 }, "id": "NKIECMJPJD_600", "locus": "GCA000828735_00585" }, { "type": "cds", "sequence": "contig_1", "start": 341434, "stop": 341619, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTAATCATTTTAATAGCAAGTGCAGTTATTGGCGTTGTTTTTTATTTAAAAAATAAAAAAAAGAATGATACAAGTGTAAATAATGATAGTTATATAATTCAAAATAATAATAATTCTTTAAAAGAACAACCTAACTCAACAAGATCATTTTTAAACCAAAAAACAATTTGTGTAGGTCATTGA", "aa": "MLIILIASAVIGVVFYLKNKKKNDTSVNNDSYIIQNNNNSLKEQPNSTRSFLNQKTICVGH", "aa_hexdigest": "a56d22efd559ac0d1495b6126dfbdd78", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6852.8047000000015, "isoelectric_point": 9.580920600891112 }, "id": "NKIECMJPJD_601", "locus": "GCA000828735_00586" }, { "type": "cds", "sequence": "contig_1", "start": 341674, "stop": 341817, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATTCTAAAAAGTAATGTTGATGTAATTGCTTTAACAGAAATTAATAGTGCAAAAAATAATGAACACGATGATTTACCAATGCGTGCCATCTTAAAAGAATTAAATTTACACAAAAATCAATTTGATCAAACAGCTAATTGA", "aa": "MILKSNVDVIALTEINSAKNNEHDDLPMRAILKELNLHKNQFDQTAN", "aa_hexdigest": "23695ef5618344d21c808dcaf0020923", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5361.030000000001, "isoelectric_point": 5.425304603576659 }, "id": "NKIECMJPJD_602", "locus": "GCA000828735_00587" }, { "type": "cds", "sequence": "contig_1", "start": 341824, "stop": 342459, "strand": "+", "frame": 1, "gene": null, "product": "Membrane nuclease A-hypothetical", "db_xrefs": [ "COG:COG2374", "COG:R", "SO:0001217", "UniRef:UniRef50_Q9PQ04", "UniRef:UniRef90_Q9PQ04" ], "nt": "GTGCTATCAAAAAACTTATTTTCAAAAGGCAATGAATCACAAACTGAACGAATTGGCTTCTTTTATAAAGCTAATATAATAAAACCAGTTCAAGAATATATTTACAAAAACAATGATAATAAAGATATTTTGTTTGAACAATATTTTAAGAACTGGAATGATAATACTAAAATTACTTATTCACGTCCGCCTTATGCCTATGAATTTCAAACTTTAGATAACAAATTTAATTTTACAATTGTCGCTAGTCATTTAGATTCACCAGGAGCAAACGAACATAAAACTAGCAAACATGATCATACTTATGGTAATAAAAATTTAAAAATTAATATTAAACAAGGCTCAAAGGAATTAAATGAGGCATATCAATTAGGAAATGTGATGGACTATATTGATAGATTAGATGGAAATAATGATGATTTAATTTTTGTTGGTGATACTAATATTAAAACAAAAAACGAATTAGGAGCCTTTGCTTCACTTTATAAACGTAATTATTTATCCAATTTTAAGGATGATAATGAAAATAACAAAACTACATTAGCAAAAACTTATTTAAAATACGCACAACACTATGACAAAATTTTTACTTCACCATCAATTAAAGTTTTAAATACTTATAAGTATGATTTATGA", "aa": "MLSKNLFSKGNESQTERIGFFYKANIIKPVQEYIYKNNDNKDILFEQYFKNWNDNTKITYSRPPYAYEFQTLDNKFNFTIVASHLDSPGANEHKTSKHDHTYGNKNLKINIKQGSKELNEAYQLGNVMDYIDRLDGNNDDLIFVGDTNIKTKNELGAFASLYKRNYLSNFKDDNENNKTTLAKTYLKYAQHYDKIFTSPSIKVLNTYKYDL", "aa_hexdigest": "1d6dd664ad380514556269f7ed3c8f3a", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 341551, "stop": 342690, "observations": { "mutation": [ 341617, 341815, 342457, 342496 ], "stop": [ 341617, 341815, 342457, 342496 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQ04", "query_cov": 1.0, "subject_cov": 0.47845804988662133, "identity": 0.991, "score": 418.0, "evalue": 1.75e-144, "gene_start": 1, "gene_end": 211, "reference_start": 154, "reference_end": 364, "reference_sequence": "MKNNKSRKWVITISLTLLIVLITSAVIGVVFYLKNKKKNDTSVNNDSYIIQNNNNNNNNNNNSLKEQPNSTRSFLNQKTICVGHWNVLNQGGINQLKNEALAKVILKSNVDVIALTEINSAKNNEHDDLPMRAILKELNLHKNQFDQTANWNYVLSKNLFSKGNESQTERIGFFYKANIIKPVQEYIYKNNDNKDILFEQYFKNSNDNTKITYSRPPYAYEFQTLDNKFNFTIVASHLDSPGANEHKTSKHDHTYGNKNLKINIKQGSKELNEAYQLGNVMDYIDRLDGNNDDLIFVGDTNIKTKNELGAFASLYKRNYLSNFKDDNENNKTTLAKTYLKYAQHYDKIFTSPSIKVLNTYKYDLWSVSLTDTLLDKNWKLAFLKTYFRSLSNQQMQNIVDNWDEIAKSNDVYIKKATSIISDHTLVAVNIKIDENDIIDEN" }, "paralog": false, "identity": 0.9841688654353562, "score": 744.0, "evalue": 2.59e-275, "description": "Internal stop codon at 341617, 341815, 342457, 342496. Nonsense mutation around 341617, 341815, 342457, 342496.", "psc": { "uniref90_id": "UniRef90_Q9PQ04", "product": "Membrane nuclease A-hypothetical", "uniref50_id": "UniRef50_Q9PQ04", "cog_id": "COG2374", "cog_category": "R", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQ04", "UniRef:UniRef50_Q9PQ04", "COG:COG2374", "COG:R" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQ04", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQ04" ], "product": "Membrane nuclease A-hypothetical" } }, "id": "NKIECMJPJD_603", "locus": "GCA000828735_00588" }, { "type": "cds", "sequence": "contig_1", "start": 342547, "stop": 342690, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAAATATTGTTGATAATTGGGATGAAATAGCAAAATCAAATGATGTATACATTAAAAAAGCTACTTCTATTATCTCAGATCATACTTTAGTTGCTGTTAATATTAAAATCGATGAAAATGATATTATTGATGAAAATTAA", "aa": "MQNIVDNWDEIAKSNDVYIKKATSIISDHTLVAVNIKIDENDIIDEN", "aa_hexdigest": "61a0e83a2488d8e34be2cb22b3dcfabe", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5358.8949, "isoelectric_point": 4.197752952575684 }, "id": "NKIECMJPJD_604", "locus": "GCA000828735_00589" }, { "type": "cds", "sequence": "contig_1", "start": 342875, "stop": 343324, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATAACTAAAAAACATTTAACTAAAATAATTACAATTTTAACTTGTAGTACTTTAATTATTGGTTTATCTTGCTTAATTGGTGCATGCACTAAATCAAAAAGTAAGGTTGATGATAAACAAGTGCTTTTTAAAATAAAAAAATTTAAAACAACTACTAATAGTATTGAGTTATTTTTAGAAGGTAGTGCAGTTTTAGCATCACATTCATACAGTGTTGTTTTAAAAGAAAAAGCAACTGGTGATTTAAAAACAATTAATAATTTAAAATTAGTTAAGGATAATGATCTTAATAAATTAATTATTAATGAATTAAAACCAAATATGGGTTATGAATTAGTAGATTTATTAATTAATAATCAAAAACAGCTAATAACTAAACTAGATTTTATAACAAGTACATTAACACAAGAAAATGTAGATCAATTTAGTATAAAAAAAATTGATTGA", "aa": "MITKKHLTKIITILTCSTLIIGLSCLIGACTKSKSKVDDKQVLFKIKKFKTTTNSIELFLEGSAVLASHSYSVVLKEKATGDLKTINNLKLVKDNDLNKLIINELKPNMGYELVDLLINNQKQLITKLDFITSTLTQENVDQFSIKKID", "aa_hexdigest": "665fd401df4a6a8f5f292f724b4595fe", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16685.54969999999, "isoelectric_point": 9.30170803070068 }, "id": "NKIECMJPJD_605", "locus": "GCA000828735_00590" }, { "type": "cds", "sequence": "contig_1", "start": 343676, "stop": 343984, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF07198.17" ], "nt": "TTGATTTTTTATTTTAAAGATAATAAGTTAGATGATTTAAATAATCAAAAATTTGTTTTAAAATTAGTTTCAGCACACCAACCATCAAATACTTTTAGTTTTGAAAATTATGTTTACAATAACTCTAAAAAGACCTTATCATTTAATTTAACGGGTTTACAAACTAGTACAAAATACTCATTAAATTCATTAACTCTTAATGGACAGCCAATATCATTAGCTAATTTAGATTTAAATATTTTAACCTCTACAAAATTAACTACTGTTCAAAGATTAGAGAACTTAGAACATGAAAGCAGTGATTTTTGA", "aa": "MIFYFKDNKLDDLNNQKFVLKLVSAHQPSNTFSFENYVYNNSKKTLSFNLTGLQTSTKYSLNSLTLNGQPISLANLDLNILTSTKLTTVQRLENLEHESSDF", "aa_hexdigest": "ebaf47bbca96fd8ac57022b462b60a91", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07198.17", "name": "DUF1410", "length": 65, "aa_cov": 0.6372549019607843, "hmm_cov": 0.7538461538461538, "evalue": 7.669435148914151e-11, "score": 37.83790969848633, "start": 9, "stop": 73 } ], "seq_stats": { "molecular_weight": 11669.967599999985, "isoelectric_point": 6.711855125427246 }, "id": "NKIECMJPJD_606", "locus": "GCA000828735_00591" }, { "type": "cds", "sequence": "contig_1", "start": 344000, "stop": 344992, "strand": "+", "frame": 2, "gene": null, "product": "DUF1410 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9X6Z3", "UniRef:UniRef90_A0AAC9X6Z3" ], "nt": "TTGAGTGTTAAGATTAAATTATATTTTGAAAATAACGTTAATTATTTAAAAAATAAATATTTAAAATTAGTATACATGGATAATGCAAATAACATCATTGTATCAAAACCTGTTTTATTTGATCCCACAAAAAAAGAGTACGAATTTAGTTTCGATAAAGTTTTATCAAACCGAAAATACACTTTTTCTAAACTAGTTTTTGATGATCTACAAGATTTTAGTGAAAAACAAGCTTTAGAAGTAAATACTCAAGGATTAAATGGTAGTTTTAATACACCTCAAGCAAAAATTAAAATCAAAAAGATTTCTTATAGTTCAACCGATAAATCCGCTAAATTAGAGTTTCATTTAGAAGATGATTTTGATTTAGTTGATGGTGATAAAGTTAGTGTCAAATATAGACTAAAACCATCATCTAATAGTATTGATACTACTAATAATACTGTTGAGGGTTTGGTTCATAACCACATATTGAATGTAACAATTAATGATTTAGAATCAAATAAATCATATGAAGTTATTGACTTTGTTATTAATGATTATAAAAATAAACAATTATTAAACAAACCTGAATTTTTAAAAAATAATACAAATCATCATAACCATCCTTTTAATATCCCGCTTGATTTTGATACTAAAAATAGTGATTATAAAACTAAATTTAATGTAAATGAAATTAGTAAATCTTATAATAAAGAAACTAAATTAACAACAATCAAATTAAAATTTGATAATATTCCCACAAATCCCACTTCATCTAATAATTTTACATTTACAATTAAAAAACGAACAGGATACACTACTAATCTGATAAGCCCAAAAACTTTGGAATATGATGTAAATAAGAAAACTTTAATAGCAAAATTTGAAAATATTGAGGACCAAATTGTTTATGATTTTGTTGATGTTAAAGTAAATAATCAAAATATTGAATTTAACAAATCATTAGATAAGTATTTTTCTATTATTCATAGTGCTGACTATCATGAATAA", "aa": "MSVKIKLYFENNVNYLKNKYLKLVYMDNANNIIVSKPVLFDPTKKEYEFSFDKVLSNRKYTFSKLVFDDLQDFSEKQALEVNTQGLNGSFNTPQAKIKIKKISYSSTDKSAKLEFHLEDDFDLVDGDKVSVKYRLKPSSNSIDTTNNTVEGLVHNHILNVTINDLESNKSYEVIDFVINDYKNKQLLNKPEFLKNNTNHHNHPFNIPLDFDTKNSDYKTKFNVNEISKSYNKETKLTTIKLKFDNIPTNPTSSNNFTFTIKKRTGYTTNLISPKTLEYDVNKKTLIAKFENIEDQIVYDFVDVKVNNQNIEFNKSLDKYFSIIHSADYHE", "aa_hexdigest": "efd6ac68426a0ddb60cda6711ddf2a34", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 343700, "stop": 344992, "observations": { "mutation": [ 343982 ], "stop": [ 343982 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAC9X6Z3", "query_cov": 1.006060606060606, "subject_cov": 0.4695898161244696, "identity": 0.958, "score": 611.0, "evalue": 1.41e-214, "gene_start": 1, "gene_end": 330, "reference_start": 376, "reference_end": 707, "reference_sequence": "MIIKKHLNKIITILTCSTLIIGLSCLIGACTKSKSKVNDKQVLFKIKKFKTTTNSIELFLEGSAVLASHSYSVVLKEKASGVLKTINNLKLVKDNDLNKLIINELKPNMGYELVDLLINNQKQLITKLDFITSTLAQENVDQFSIKKIDWKNITNNSADLNLEFTKYFLANENQNIFEISLLNLQTQQITKFKFNYQPNSPSITFNFTQLENNAKYQIHRIVLNNKELVFSNQNLVLSNTNKQNSNPIIKDLNITKIEKEIYDTSAKLIFYFKDNKLGDLNNQKFVLRLVSAHQPSNTFSFENYVYNNSKKTLSFNLTGLQTSTKYSLNSLTLNEQPISLANLDLNILTSTKLTTVQRLENLEHESSDFWFNDENLSVKIKLYFENNVNYLKNKYLKLVYMDNANNIIVSKPVLFDPTKKEYEFSFDKVLSNRKYTFFKLVFDDLQDFSEKQALEVNTQGLNNSFNTPQAKIKIKKISYSSTDKSAKLEFHLEDDFDLVDGDKVSVKYRLKPSSNSIDTTNNTIEGFVHNHILNVTINNLESNKSYEVIDFVINDYKNKQLLKEPEFLKDNTNHHNHPFNIPLDFDTKNSDYKTKFNVNEISKSYNKETKLTTIKLKFDNIPTNPTSSNNFTFTFTIKKRTGYKTNPISPKTLEYDVNKKTLIAKFENIEDQIVYEFVDVKVNNQNIEFNKSLDKYFSIIHSADYHE" }, "paralog": false, "identity": 0.9606481481481481, "score": 824.0, "evalue": 7.64e-301, "description": "Internal stop codon at 343982. Nonsense mutation around 343982.", "psc": { "uniref90_id": "UniRef90_A0AAC9X6Z3", "product": "DUF1410 domain-containing protein", "uniref50_id": "UniRef50_A0AAC9X6Z3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAC9X6Z3", "UniRef:UniRef50_A0AAC9X6Z3" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAC9X6Z3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9X6Z3" ], "product": "DUF1410 domain-containing protein" } }, "id": "NKIECMJPJD_607", "locus": "GCA000828735_00592" }, { "type": "cds", "sequence": "contig_1", "start": 344996, "stop": 346105, "strand": "-", "frame": 2, "gene": "ackA", "product": "Acetate kinase", "db_xrefs": [ "COG:C", "COG:COG0282", "EC:2.7.2.1", "KEGG:K00925", "SO:0001217", "UniRef:UniRef50_Q9PQ06", "UniRef:UniRef90_Q9PQ06" ], "nt": "ATGAATTTAAAACAAATATTTAGTGGCAAAATTCGCTTAGACGTTAGCCCACACCAACTAAAATATATCTTTGATAAAAATGAATATGTTTATTTACTTGATGATGATCACAACAATCACGTTGATTTAATCATTAAATTTTTAATTAACAAAAAAATTATTAAAAAATTAGAAGATATCACTTATTTTGGAATTCGAGCTGTTTATGTTAAAAATTTTTATGGAGCAAGTGCTTTTTTAAATAATGAAATTTATAATGATCTTAACCAACACACAGATTTATGTCCTATTCACAACCAAAATACTATCATGTTAATTAATGACATTAAGCATTTAAACACCTCTGCAAAAATTATTATTATTTTTGATAATTGGTTTCATCGTAGTATTAATAAAAATAAATACACTATTGCTATTGATCAAACAATAGCAATAAAACATAAAATTCGTAAATATGGTTTTCATGGAATTTCTTTTAGTTATAGTAATAAAATAATGCAAAATTATTTAGGAAAACAAGATGTTAATTTAATTATTTTACACTTAGGTTCTGGATCGTCAGTTTGTGCAATTAAAAATGGAAAATCATTTGATACCTCAATGTCTTACTCTCCTCTTTCAGGAATTATTATGAATACAAGAAGTGGTGATATAGACCCTAGTGTTTTACTTACTTTAAAAAAAGTAAAAAACCAATCACTGAAGCAATTGATCTTATTTTTAAATCAGCAATCAGGTCTTTATGGATTAACAAAACATCATAGTATTAGTGAAGTTGCTAATAATTTGCACAATAAAAATAATGAACTTGCCCTTGAGATGTACACTGATAGCATTGTTGATTATTTTATTAAGTATTTAAACCATTTACAAAAGATTGATGCAATTGTATTTTCGGGAGGAATTAGTGAATTTGAGCATCTTGTTTTAGAAAAAATTATTAGTAAAATTCATCTAATAAACCTTGAAATTAACGATCATTTAATACTAACAAACACGTTAAATAAAATTTCAACATCTAAATCACAAATTCCTATATATTTATGTTTAGTGAATGAAGAGCACGAAATTGCTTATGAAATGCTAAATTTAATTAATAAGACAAAATAA", "aa": "MNLKQIFSGKIRLDVSPHQLKYIFDKNEYVYLLDDDHNNHVDLIIKFLINKKIIKKLEDITYFGIRAVYVKNFYGASAFLNNEIYNDLNQHTDLCPIHNQNTIMLINDIKHLNTSAKIIIIFDNWFHRSINKNKYTIAIDQTIAIKHKIRKYGFHGISFSYSNKIMQNYLGKQDVNLIILHLGSGSSVCAIKNGKSFDTSMSYSPLSGIIMNTRSGDIDPSVLLTLKKVKNQSLKQLILFLNQQSGLYGLTKHHSISEVANNLHNKNNELALEMYTDSIVDYFIKYLNHLQKIDAIVFSGGISEFEHLVLEKIISKIHLINLEINDHLILTNTLNKISTSKSQIPIYLCLVNEEHEIAYEMLNLINKTK", "aa_hexdigest": "54d8ae14ddcbbb03fddbd9065c5cd6c6", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_Q9PQ06", "query_cov": 1.0, "subject_cov": 0.9534883720930233, "identity": 0.978, "score": 663.0, "evalue": 3.48e-239, "valid": true, "gene": "AckA", "product": "Acetate kinase", "ec_ids": [ "2.7.2.1" ], "uniref50_id": "UniRef50_Q9PQ06", "cog_id": "COG0282", "cog_category": "C", "kegg_orthology_id": "K00925" }, "pscc": { "uniref50_id": "UniRef50_Q9PQ06", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQ06" ], "product": "Acetate kinase" }, "genes": [ "ackA" ], "id": "NKIECMJPJD_608", "locus": "GCA000828735_00593" }, { "type": "cds", "sequence": "contig_1", "start": 346431, "stop": 347963, "strand": "+", "frame": 3, "gene": null, "product": "DUF1410 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9X823", "UniRef:UniRef90_A0AAC9X823" ], "nt": "ATGAAATTAATTAAGAAAAAGTTATTATTTTTATTTACAGCTGCTTTAGTTCCTATTGTTGCTACAGCACTTGCATCTTGTAGCAATAAATCAAAGTTAAATGTTAATGTTTTAATGAAAGATATAATTCATACCAAGAGTGACCATTATTATGTTGTTTATGAAGTTAATGAACTAAATACTTCGCAAGGTATTTATAAGAATTTAACTTTAAACACCAATCTTTTTAATCCATTGTTAAATAAAAAAGTTGACCGTTTAGAAAATATTCATCCTTTGATTATTAATAACAAAATTTATTTAAGACTAGCTCGTAAACCAAAAATTAATGATCATTTTATCACTAATTTTTCAAATGATGCATTTCCTGCACTACATTTAATTTTTGATCATAATTTAAATTTTATCGAACAAGATATTAATGAAGATTTAAATTTACAACCATCACTTCCTAATACACCAAAGATTGATCATTTAGAAATTAATAATATTTCGTTTACAAATATTAAACAAAACCAAGTTGATATAAAGATTAAGTTAAAAACAGTTTCTTTTAAAGACGATGCTATTCATAATTTTAGTTTAAAAATTATCAAAAAAACTAATCACCAAATCATTACTAGTGATGCTGTTTTCAATAAGCAAAAAAATGAAATTGATGCTACCGTTTATAATTTAACATCTGATTGTGAATATTTATTAGATAAACTTAATTTTAATAATCAAAAAATTCAATTAACTTCAATTCCAAACCACACTTTTAAAACCCTTGATGATAGTGATAATCTTTTAAAAAATCTAGAAATTACAAAGATTAATGATAATGGTGCTAATTTAACTTTAAATTTTAAAGAGTTAAAAATAAAAGATGATAGTGATGAAGCTTTAAATAATATCCACATTAGTTTAGCATATAATAGAGTTTATAAAAAAGGTCAATTAGAGCTTAATAAGAAGCATTTTTTTATTGATAAATTAAACAAGAGAATTATTGTTTATGTTGATGAATTAGAAAGAGGAACTCATTATGATATTAAACAAATTAAGATTAATGATCAAGTTTTAAATTTAAATAATCAAACACATGATTTTACAACAAAAGGCGATTATGCTAAAGTTGAAGCTATTGATTATGAAACAGCTGTTGATAAAATTACAAAAGCAGTTACAATTAAAGTAAAATTATCTGAAGATCTTGCTTTCTACAATCCTAATATTCATAATTTTAAATTTTTAATTAGAAAAGAAAATGATCAAAACCCAATTGAATTCTCAGAAGTTAAATACGATGAACAAGAACGTTTATTAACTGCTGTAATTAATGGTTTAGATGTTGACAAAACTTATGAAATTGATAATATTTTACTAAACGGTAAACCAGTATCTTTCCCTAATGATGATGACTATGCAGGTGGATATTATGGACCTATTAACAAAACATTTAAAATACGTCCTGATCGATCAAGTATTGAAATTTTTAAAGATAAATCATTCTATAATTCTTACTATAGTGCTTTTAATAGTTGGTTTTGA", "aa": "MKLIKKKLLFLFTAALVPIVATALASCSNKSKLNVNVLMKDIIHTKSDHYYVVYEVNELNTSQGIYKNLTLNTNLFNPLLNKKVDRLENIHPLIINNKIYLRLARKPKINDHFITNFSNDAFPALHLIFDHNLNFIEQDINEDLNLQPSLPNTPKIDHLEINNISFTNIKQNQVDIKIKLKTVSFKDDAIHNFSLKIIKKTNHQIITSDAVFNKQKNEIDATVYNLTSDCEYLLDKLNFNNQKIQLTSIPNHTFKTLDDSDNLLKNLEITKINDNGANLTLNFKELKIKDDSDEALNNIHISLAYNRVYKKGQLELNKKHFFIDKLNKRIIVYVDELERGTHYDIKQIKINDQVLNLNNQTHDFTTKGDYAKVEAIDYETAVDKITKAVTIKVKLSEDLAFYNPNIHNFKFLIRKENDQNPIEFSEVKYDEQERLLTAVINGLDVDKTYEIDNILLNGKPVSFPNDDDYAGGYYGPINKTFKIRPDRSSIEIFKDKSFYNSYYSAFNSWF", "aa_hexdigest": "77e57e0665c90af42b94a41a63453e9c", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 346431, "stop": 348266, "observations": { "mutation": [ 347961, 348165 ], "stop": [ 347961, 348165 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAC9X823", "query_cov": 1.0, "subject_cov": 0.46195652173913043, "identity": 0.996, "score": 993.0, "evalue": 0.0, "gene_start": 1, "gene_end": 510, "reference_start": 1, "reference_end": 510, "reference_sequence": "MKLIKKKLLFLFTAALVPIVATALASCSNKSKLNVNVLMKDIIHTKSDHYYVVYEVNELNTSQGIYKNLTLNTNLFNPLLNKKVDRLENIHPLIINNKIYLRLARKPKINDHFITNFSNDAFPALHLIFDHNLNFIEQDINEDLNLQASLPNTPKIDHLEINNISFTNIKQNQVDIKIKLKTVSFKDDAIHNFSLKIIKKTNHQIITSDAVFNKQKNEIDATVYNLTSDCEYLLDKLNFNNQKIQLTSIPNHTFKTLDDSDNLLKNLEITKINDNGANLTLNFKELKIKDDSDEALNNIHISLAYNRVYKKGQLELNKKHFFIDKLNKRIIVYVDELERGTHYDIKQIKINDQVLNLNNQTHDFTTKGDYAKVEAIDYETAVDKITKAVTIKVKLSEDLAFYNPNIHNFKFLIRKENDQNPIEFSEVKYDEQERLLTAVINGLDVDKTYEIDNILLNGKPVSFPNDDDYAGGYYGPINKTFKIRPDRSSIEIFKDKSFYNSYYSVFNSWFWYLSVSKKDDNERSKEVKKDYKEHLFISDITKNSAIINIKCEKLLLRKENSLKPKIKLRYHPLNNPNEWAYKEFSFEDKNCFMSIDEDQKTIKIKLLNLDNHINYEFVSFILEDVDGNYHSDNFNVNVYIGDFFDSHPFHLDLSFLKFKTNEFKKAVFSFTNTKSTSTDLNVDLQDNQYADSIEHKIKYTLEDENHNKYKFVKNLSKSETTQLNATKNIILHLFALDKNQTFKLISFEIDDDNYLTNNITVKTLNESSPHLLLNLEFNDIKNTNSTLKLTLNQAVINMPKRVDVILKNKKTNKLLYLFKTDFYFDKKTHQILTKLDNLNINTDYEISELIIDKQQINLKNINNKTFKTLDEGFDQFYLDKIDHEINQQQKNIKLKLHFSDFIFKDKNAHKMSLVLEDENNQQESIDLNTDNQLKIDYEHQTIEFELKNLKPDIQYRINSIKLNNKNLDLTWVDNQRYFNFPGVLNPNLKWKFDNIHYESVSESNVKINITFDQDIFLDNLPQDKDCNIELNYVYNTNGGDTLEQKIIAKIVHKNHVEITLTKQNCFWLDDLVYNYNHNSASDFERLLKSIKINGHKITKVNEGN" }, "paralog": false, "identity": 0.9934533551554828, "score": 1213.0, "evalue": 0.0, "description": "Internal stop codon at 347961, 348165. Nonsense mutation around 347961, 348165.", "psc": { "uniref90_id": "UniRef90_A0AAC9X823", "product": "DUF1410 domain-containing protein", "uniref50_id": "UniRef50_A0AAC9X823", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAC9X823", "UniRef:UniRef50_A0AAC9X823" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAC9X823", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9X823" ], "product": "DUF1410 domain-containing protein" } }, "id": "NKIECMJPJD_609", "locus": "GCA000828735_00594" }, { "type": "cds", "sequence": "contig_1", "start": 348207, "stop": 349343, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF07198.17" ], "nt": "ATGAGTATTGATGAAGATCAAAAAACAATTAAAATTAAATTATTAAATTTAGATAATCATATTAATTATGAATTTGTAAGTTTTATCTTAGAAGATGTTGATGGTAATTACCATAGTGATAATTTTAATGTAAATGTTTATATAGGAGATTTTTTTGATTCTCATCCATTTCACTTGGATCTTTCATTTCTTAAATTTAAAACAAATGAGTTCAAAAAAGCTGTTTTTTCATTCACAAATACAAAATCAACTTCAACCGATCTAAATGTTGATTTACAAGACAATCAATATGCTGATAGTATTGAGCATAAAATAAAATATACTTTAGAAGATGAAAATCATAATAAATATAAGTTTGTTAAAAACTTATCAAAATCAGAAACTACTCAACTTAATGCAACAAAAAACATTATTTTACATCTTTTTGCTTTAGATAAAAATCAAACATTTAAACTTATTTCTTTTGAAATAGATGATGACAATTATTTAACTAATAATATTACAGTTAAAACACTTAATGAATCATCTCCACATTTATTATTAAATTTAGAATTTAATGATATTAAAAATACAAATAGTACTTTAAAATTAACACTAAACCAAGCAGTTATAAATATGCCAAAAAGAGTTGATGTTATTTTAAAAAATAAAAAAACAAACAAGCTGTTGTATTTATTTAAAACAGATTTTTATTTTGATAAAAAAACACACCAAATCTCAACTAAATTAGATAACTTAAACATTAATACTGATTATGAAATTAGTGAACTTTTTATTGATAAGCAACAGATTAATTTAAAAAATATCAATAATAAGACTTTTAAAACTTTAGATTACGGCTTTGATCAATTTTACTTAGATAAAATTGATCATGAAATTAACCAACAACAAAAAAACATTAAATTCAAGTTGCATTTTAGCGATTTCATCTTTAAAGATAAAAACGCCCATAAAATGTCATTAGTGTTAGAAGATGAAAACAACCAACAAGAATGTATTGATTTAAATGCTCATAACCAATTAAAAATCGACTATGAGCACCAAACAATTGAATTTGAATTAAAATATTTAAAGCCTGATATTAAATATCGAATTAATTCAATTCAATTGAATGATAAAAATTTAGATTTAACTTGA", "aa": "MSIDEDQKTIKIKLLNLDNHINYEFVSFILEDVDGNYHSDNFNVNVYIGDFFDSHPFHLDLSFLKFKTNEFKKAVFSFTNTKSTSTDLNVDLQDNQYADSIEHKIKYTLEDENHNKYKFVKNLSKSETTQLNATKNIILHLFALDKNQTFKLISFEIDDDNYLTNNITVKTLNESSPHLLLNLEFNDIKNTNSTLKLTLNQAVINMPKRVDVILKNKKTNKLLYLFKTDFYFDKKTHQISTKLDNLNINTDYEISELFIDKQQINLKNINNKTFKTLDYGFDQFYLDKIDHEINQQQKNIKFKLHFSDFIFKDKNAHKMSLVLEDENNQQECIDLNAHNQLKIDYEHQTIEFELKYLKPDIKYRINSIQLNDKNLDLT", "aa_hexdigest": "ef3ab30f2394b3220d39239f1defac22", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07198.17", "name": "DUF1410", "length": 80, "aa_cov": 0.21164021164021163, "hmm_cov": 0.7, "evalue": 5.416894932056358e-09, "score": 31.91581916809082, "start": 298, "stop": 377 } ], "seq_stats": { "molecular_weight": 44808.959300000024, "isoelectric_point": 5.788733100891113 }, "id": "NKIECMJPJD_610", "locus": "GCA000828735_00595" }, { "type": "cds", "sequence": "contig_1", "start": 350777, "stop": 353149, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF07198.17" ], "nt": "TTGCCAACAGCAGTTTTTAAAGATGAAAATAATAAAGAATATTTAATACCAGCGTTGAGTGTAAAAAATAATGTTGGGTATTTTAATACTAAAAAACTACCTTTAAATCACAAATATGAATTAAACCGAATTGTTAACAATAATGAATTAAATGATGTTTTAGTGCCAAATAATGAATTAATGCTTGAACATAAAGTTAGTGTAAATAAACCAACAAAAGCTAAAGTTAATTTTATTGATAACAAAAAAGTATATGAAGTTGATTTAGGTGTTCAATTAAAAAACACTCCTCTTGAACTTACCTTAGAAGATCTAAATCATCAAACATATAAAATTAATGCAAAAACAGATGAAAAAGGACGAGCTGTTTTTGATATTTCATCTTTAGGTGATAATAATTTATATGAAGTTATTGGTATTAAAAAAACTAATGAAGTTGATGTTGTTAATCTTAAACAGATTCCATACCATAATCGATCAATTAATAATTTAAATTCAAACGCATTAAATACCCCATATCAATATACAAAAAATGGTGATATTAATTTAATTGCTAAAGTAGCGCCATATTATGTTAACCAACAAGTATATGGAATTTTTAAAGATCAAAATAATCAAGAACACCAAATTTTAGCAAAAGTTAAAAAAGATGGAACCATAGCATTTGATACAGGTGCATTAAATAATAACAGTAATTATTCACTAGACAAAATTGTTTCAGTTTCAAACCCACAAAATGTTTTGGTTTCAAATTTTGATTTAACTTCAAAACAAAAACAATTAATTAAAAAGCCAGCTGCTAATGCAAGTGTTGATTCAACTAAAAAAACCCAAATTTTAGAAAATTTAAATAATGATTTAATTAATCAGAAATTAGTAGCAACTTTTGTTGATAATAATGATAAAGAATATAAAGTAGTTGCTAATGTGGATCAAAATAATAAAATTATTTTTGATAGTAATGATTTACCAAAAGGATATATTTATCATTTAGCTAAAGTTGAAAATAACGATTTAAATAAAGTCATTAATCTTAATGATTTTGAACTAAAAGATAAAATAATTGATAAACGAGATTTGAATTTATTAGATTCACATCCAGATTTTACGTACGATAATGATGGAAATTTAGAAATTCACACTCAATTAGCAAATGATTTAAATGATGATTTAAAACAAAAAGCTTTAAATAATGCAAATGTCAAAGGAATTGTTGTTGATCAAGATGGTATAGAACATGAGATAGATGTAAGTATTGATGCTAATGGCAAGGTTATTATTCCAACAAAAAATTTAGCAAATAATGACCCAACTAAACCTAATATATACACACTTAAAAAAGTCGTTTTAAAACAAAATAATCAACCAAATATTGATCTAATTAGTGAAGAGCAATTAAGTGGTGATAATCATATTTCATTTAAAAAACCAACAATAACTGCTAAAACTAAAGAAAATGATGATTATGAAATTAGTTTTTCAAATCCTAGTTTAGCAAATAAAAAAATTAAATTAACATTTAAAACCGATGATAATAATACTAATACAAAAACGGTTGAAGCTAGTATTGGTTTAGATGGAAAAGCTATTTTTAAAACAAGTGATGATGCAATTTTTGCACCAGATCATAAATATACACTAACAAAAATTGAAGCTGATAATAAAAAAGTTGCTAATATTGATGAAATTTCACCATTAGATCGAATAATTAATAAACAAAAAAATGGTAATGTTAATGCTGATAATAAGCATGAGTTTAAAATACCAGATCAAAAAAATAAAAATTTAATTGCTATTTATAAAGATAAAAATAACAATGAAATTCATGTTCCAATCAAAACTGATGATAAGGGAAAAGTTATTGTTGATCCAAACAATAATTTATTTGATCCTAACAAAATTTATGATTTTGATAAAATTGTAGATTTAAATGAATATCCAAACAAAACAATATTAGATAGAAATAGTATTAACAAAGATGTTAGTGCAATTAATGATGGTGTCGATCAAGCTCGTAAGCTTGTTTTAAAAGCACCAGCAGTTTCTAACATAACAGCAAATGCTATTAATTTCCAAGTAAACTTATTTGATTTAAATAAACTATCTTATAATCGTCAATTTGCACTAACAATTGCTAAAGTTAATGATTTAAATGATACACAAAAATACATTGCAACTTATGATCCAAAAACTAACAACTACAAACTAAATTTTGATTTTACACACTTAGATGCAAACACAAAATATAAAGTTATTGATGTTGAATTATTAAAATTAAACAACAAGGAAAAGCCAATAAAATTAGTTAAAGATGATGTTTTAAATTTTGAATTCACAACAAGCCCAGCAACTACTATTAATCCACCAATTTGA", "aa": "MPTAVFKDENNKEYLIPALSVKNNVGYFNTKKLPLNHKYELNRIVNNNELNDVLVPNNELMLEHKVSVNKPTKAKVNFIDNKKVYEVDLGVQLKNTPLELTLEDLNHQTYKINAKTDEKGRAVFDISSLGDNNLYEVIGIKKTNEVDVVNLKQIPYHNRSINNLNSNALNTPYQYTKNGDINLIAKVAPYYVNQQVYGIFKDQNNQEHQILAKVKKDGTIAFDTGALNNNSNYSLDKIVSVSNPQNVLVSNFDLTSKQKQLIKKPAANASVDSTKKTQILENLNNDLINQKLVATFVDNNDKEYKVVANVDQNNKIIFDSNDLPKGYIYHLAKVENNDLNKVINLNDFELKDKIIDKRDLNLLDSHPDFTYDNDGNLEIHTQLANDLNDDLKQKALNNANVKGIVVDQDGIEHEIDVSIDANGKVIIPTKNLANNDPTKPNIYTLKKVVLKQNNQPNIDLISEEQLSGDNHISFKKPTITAKTKENDDYEISFSNPSLANKKIKLTFKTDDNNTNTKTVEASIGLDGKAIFKTSDDAIFAPDHKYTLTKIEADNKKVANIDEISPLDRIINKQKNGNVNADNKHEFKIPDQKNKNLIAIYKDKNNNEIHVPIKTDDKGKVIVDPNNNLFDPNKIYDFDKIVDLNEYPNKTILDRNSINKDVSAINDGVDQARKLVLKAPAVSNITANAINFQVNLFDLNKLSYNRQFALTIAKVNDLNDTQKYIATYDPKTNNYKLNFDFTHLDANTKYKVIDVELLKLNNKEKPIKLVKDDVLNFEFTTSPATTINPPI", "aa_hexdigest": "d2ad901a87a1a6bb64d848117c99fd35", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07198.17", "name": "DUF1410", "length": 60, "aa_cov": 0.07468354430379746, "hmm_cov": 0.9833333333333333, "evalue": 1.8228420819083436e-77, "score": 251.2268829345703, "start": 189, "stop": 247 } ], "seq_stats": { "molecular_weight": 89430.58170000045, "isoelectric_point": 6.323758506774904 }, "id": "NKIECMJPJD_611", "locus": "GCA000828735_00596" }, { "type": "cds", "sequence": "contig_1", "start": 353264, "stop": 353854, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATGATGATTTATTAGATACAGATGTTATTAACCCTTTAGTTAATTTTAGTAAAACTAATAAAATCGCTTCAATTAATGGTCTTGATTTAAAAGGAAATAGTAAATATAGTATTAAAAACTTATATTATTTAAATGACCAAAACCAAAAGGTGTATTTATTTAAAAATGATGTTACAAAATATGAACAATATTTTATTACAAATCCTCATAAGATCAATTTATCATTTAATAAAAGCGCTGCAGAACAAGATATTTTTGCAGACCATGCTAATTTATTTATTGATTATAAAGATCATGATCAAAAATTACGAATTAATGATGATGTTAAAATTTATTATCAAAATATTGATAACATTAAAAATGAACTACAAATTGGATATGGAAAAGTTGTTGTAAATAATAAAATTAAATTCAATTTGGTTGGTTTAAAAGAAAAAACAACATATGTTATTAAAAAACTTGAAGCTTTAAATAAGAGTGCAAGCAGTATTGTTAATAGTGAATTTGATTTACTAGATCCAAATACTAATTTTAGTACATCTAATAAAAACACAACCCTAGTGGGTCTAAATTCAATTGATAATTGA", "aa": "MDDDLLDTDVINPLVNFSKTNKIASINGLDLKGNSKYSIKNLYYLNDQNQKVYLFKNDVTKYEQYFITNPHKINLSFNKSAAEQDIFADHANLFIDYKDHDQKLRINDDVKIYYQNIDNIKNELQIGYGKVVVNNKIKFNLVGLKEKTTYVIKKLEALNKSASSIVNSEFDLLDPNTNFSTSNKNTTLVGLNSIDN", "aa_hexdigest": "eaf7d9e31badd70e4d200ebadcd6ed09", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 22488.014800000023, "isoelectric_point": 6.295452690124511 }, "id": "NKIECMJPJD_612", "locus": "GCA000828735_00597" }, { "type": "cds", "sequence": "contig_1", "start": 354407, "stop": 354679, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATTAAACAAATTAGTTTAACTCAAAAACCAACTAATGCTCATTTCAATTTTGCAAATAATAAAACTAATAACATTGTTTATTCATATGATGAACAAAATAAAATTAGTTTATCAAACAATATTATTGCAACATCTTATAGTCTACAAACGATTAATAATAATAAAAAATCAGTTGATATTAAGGTTGGTTTACAAGCTGATAATCAACTTTTATTAAATCAATATTTACGTTTAAAATTCAAACGTTTAAATGATAATGCAATTGTTTGA", "aa": "MIKQISLTQKPTNAHFNFANNKTNNIVYSYDEQNKISLSNNIIATSYSLQTINNNKKSVDIKVGLQADNQLLLNQYLRLKFKRLNDNAIV", "aa_hexdigest": "74d4ef5e0d0a9a775b34942deb8609ca", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10329.631499999992, "isoelectric_point": 9.7943754196167 }, "id": "NKIECMJPJD_613", "locus": "GCA000828735_00598" }, { "type": "cds", "sequence": "contig_1", "start": 355151, "stop": 355351, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTTAAAAATAAACCAAATAAGGCATACAAACCATTAAACAACAATAACATTATTTTTGAACATAAAGACAATACTCAAGCTAAAGAGTTCTCTACAACAAAACAAGATCACAAAGTGATTAGTGTTACATCAAGTGTTTCAACCAATACAACACAACAAGAAATAACTGTTAAAATAGATGGTATTCAACGTGCATGA", "aa": "MFKNKPNKAYKPLNNNNIIFEHKDNTQAKEFSTTKQDHKVISVTSSVSTNTTQQEITVKIDGIQRA", "aa_hexdigest": "3721447e7a618d39a0c067c7c01e1dd9", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7521.352200000002, "isoelectric_point": 9.631721687316894 }, "id": "NKIECMJPJD_614", "locus": "GCA000828735_00599" }, { "type": "cds", "sequence": "contig_1", "start": 355772, "stop": 356359, "strand": "+", "frame": 2, "gene": null, "product": "DUF1410 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215CB5C8", "UniRef:UniRef90_UPI00215CB5C8" ], "nt": "ATGGTTGATTCACAAAATCAAAAATACTTACAAGCAACATTAACAAATTTAGTTTTTAATCAAGATACTATTATTAAAAAAATTGAATTCAAAAATTTAAATCAAACTTTTACTAAAGTAGGTAAAAATAACACTAATGTAATCTATGATGAAAATGATGTAAATAAATTAAAAATTAATAATGATTTTCAAATTGTGGGACCACTATCAACTGATACTAATAACACACAAAATATAGTGGCTAATAACAAACATGTAATTTCTTCAACACTAGATTTTAAAGTTAATCCACATATTAGTAAGAATTTGAAATTTAAACTTAAATTTCAAAATATGAATGGTGAAGTTGTTTATAGTCCAATTTTAACTAATAGTTCAATTATAGTTAATAACAATAAAAACGTAATTAATTTTACTTTAGCTAATTTAAAATCAAATCAATTATATAGATTAGTTGATGTTTATTATATTGATGATAATAATGATACTATTAATGACAAAAACAAAGTACCAAAAGCAAATGATGTAACTCGCATAATTAATATTGCAGCTGATAAAACAACAATTACTAGATCAAACAATAATTGA", "aa": "MVDSQNQKYLQATLTNLVFNQDTIIKKIEFKNLNQTFTKVGKNNTNVIYDENDVNKLKINNDFQIVGPLSTDTNNTQNIVANNKHVISSTLDFKVNPHISKNLKFKLKFQNMNGEVVYSPILTNSSIIVNNNKNVINFTLANLKSNQLYRLVDVYYIDDNNDTINDKNKVPKANDVTRIINIAADKTTITRSNNN", "aa_hexdigest": "fcba299afa3780c3f461d9b8a7027ec9", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 355880, "stop": 356605, "observations": { "mutation": [ 356357 ], "stop": [ 356357 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00215CB5C8", "query_cov": 0.8153846153846154, "subject_cov": 0.6597510373443983, "identity": 0.975, "score": 293.0, "evalue": 2.72e-98, "gene_start": 37, "gene_end": 195, "reference_start": 1, "reference_end": 159, "reference_sequence": "FTKVGKNNTNVIYDENNLNKLIINNDFQIVGPLSTDANNTQNIVANNKHVISSTLDFKVNPHISKNLKFKLKFQNMNGEVVYSPILTNSSIIVNNNKNVINFTLANLKSNQLYRLVDVYYIDDNNDTINDKNKVPKANDVTRIINIAADKTTITRSNNNWNSTATSSQFEFEVSSEDGNDVLENLKATIIFKKDQTLLTPIEVNITKENDKYLIKGEITNLEPENTYTLESIVLAKPSKTN" }, "paralog": false, "identity": 0.979253112033195, "score": 465.0, "evalue": 1.47e-168, "description": "Internal stop codon at 356357. Nonsense mutation around 356357.", "psc": { "uniref90_id": "UniRef90_UPI00215CB5C8", "product": "DUF1410 domain-containing protein", "uniref50_id": "UniRef50_UPI00215CB5C8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00215CB5C8", "UniRef:UniRef50_UPI00215CB5C8" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00215CB5C8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215CB5C8" ], "product": "DUF1410 domain-containing protein" } }, "id": "NKIECMJPJD_615", "locus": "GCA000828735_00600" }, { "type": "cds", "sequence": "contig_1", "start": 356516, "stop": 356758, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTAAAGGTGAAATTACTAATTTAGAACCAGAAAATACGTATACACTTGAGTCAATTGTCTTAGCTAAACCAAGCAAAACAAATGGCCCATTAAATGTTGAAATCACAAATAAAGATAACATTTCATTTGAAACAAAAGCTGGTAATTATAAAGTTATTCAAATTAAATCACAAAATCCATCCACAGCAAATACTAAACAAAAAATTACTTTAAAAGTTGATGGTGTGCAAAATGCTTGA", "aa": "MIKGEITNLEPENTYTLESIVLAKPSKTNGPLNVEITNKDNISFETKAGNYKVIQIKSQNPSTANTKQKITLKVDGVQNA", "aa_hexdigest": "1e5da9317a4f3e0374246ebd566df41f", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8775.859099999998, "isoelectric_point": 8.96376476287842 }, "id": "NKIECMJPJD_616", "locus": "GCA000828735_00601" }, { "type": "cds", "sequence": "contig_1", "start": 358661, "stop": 358855, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCGAAAACCTACACTAGCTCATACTAATATTAATAGTAATGAAAACAATGTTGTTTTTGAAGATGATAATTCAAATCATAATTTTAAAACATTAACTGTTGATCATAAGGTTACAAGCATTAATTCTAGTGATAATATAAGCACAACAACACAAACAGTAAATATTAATATTGATGGTATTCAACGAACTTGA", "aa": "MRKPTLAHTNINSNENNVVFEDDNSNHNFKTLTVDHKVTSINSSDNISTTTQTVNINIDGIQRT", "aa_hexdigest": "3b9335f70d5b81e6b3e7871d0f44262f", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7170.682700000002, "isoelectric_point": 5.765770149230956 }, "id": "NKIECMJPJD_617", "locus": "GCA000828735_00602" }, { "type": "cds", "sequence": "contig_1", "start": 359726, "stop": 360019, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTATTATTTTATTGATGCAAATAATACAAATGTTGTTGAAAACCAAAACTTTGCAAAAGCTAATAATGTTAAACGAACAATTAATATTACACCTGGTGATACAACAATCACGAAAGCAGATGATTTTAGTAGTAATATAACAGCAAATAGTGCAAAAATTAAATTCGAAGTTAGAAGTGGAGATGAAGGAGCTTTAAGCAATACAATTGAAGCAACTGTTGTTTTTAAAAATCAAAACAATGAACAAACAACTCCTATTAAAACATCTTTAAAACAAATTAATGGTAAATGA", "aa": "MYYFIDANNTNVVENQNFAKANNVKRTINITPGDTTITKADDFSSNITANSAKIKFEVRSGDEGALSNTIEATVVFKNQNNEQTTPIKTSLKQINGK", "aa_hexdigest": "946016616faa424a253db3ce250eadec", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10688.70059999999, "isoelectric_point": 7.963215446472168 }, "id": "NKIECMJPJD_618", "locus": "GCA000828735_00603" }, { "type": "cds", "sequence": "contig_1", "start": 360061, "stop": 360186, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAGTAATTTTAAAATTTCCAGCAGCAGTTTTAAAATTAATATTTGCTTTATTATTAATATTAACTTTAAGGTTGTTAGCAGTATTTGTTGGTTTTGATAAACTAATACTATCTAATGTATAA", "aa": "MLVILKFPAAVLKLIFALLLILTLRLLAVFVGFDKLILSNV", "aa_hexdigest": "f803b855a1beb310aec0a57e52b1fee0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4553.792599999999, "isoelectric_point": 10.288976860046386 }, "id": "NKIECMJPJD_619", "locus": "GCA000828735_00604" }, { "type": "cds", "sequence": "contig_1", "start": 360644, "stop": 361036, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGCAAAATTATTGTTGATAGTAGTAAAAATAAAAAATATCTTGAAGCAACACTTAATGATTTAGTATTAAATGAAGACACTATTATTAATAAAATTGAATTTATTACAAGACCAAAGAAGGCTGCTGATTTTGTTGGTGCAAATAGTTCTAATACAATTTATGAATTAACAAATAATACCCAAAATAATCAAAAACTTAAAGTTAATAATGATTTTATTATTAGTGGTCCTCTTGCAAGTGATAATAATGCAATTGAGACAAAAGAATATGAACAAAAAAATAATGTTAGTTCATCAATTACACTAAAAGCAAATCCATACATTATTAAAAATTTACAATTCAAATTAAAATTCACAAATCAAAATGATGAAAGTGTAGAAAGCGATTGA", "aa": "MGKIIVDSSKNKKYLEATLNDLVLNEDTIINKIEFITRPKKAADFVGANSSNTIYELTNNTQNNQKLKVNNDFIISGPLASDNNAIETKEYEQKNNVSSSITLKANPYIIKNLQFKLKFTNQNDESVESD", "aa_hexdigest": "b293da6408f1f88c8c6f68d01a040f6c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14647.20749999998, "isoelectric_point": 5.533867073059081 }, "id": "NKIECMJPJD_620", "locus": "GCA000828735_00605" }, { "type": "cds", "sequence": "contig_1", "start": 361366, "stop": 361539, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTTTGTCTGTAATTTCAACAATAAGATTATTAGTTGTTTTAGTTGGTTTGCTTAATTCAATAGTTTCTAAACTATATTTTGTATTTGGGTCTAAATTAGATATTTTTCCTTTAATTAATCACTTATCTTTTTCTTTAGTGATTGTTGCTATTGTTGAAATAATTTGTTGA", "aa": "MFLSVISTIRLLVVLVGLLNSIVSKLYFVFGSKLDIFPLINHLSFSLVIVAIVEIIC", "aa_hexdigest": "71341e7ee9f0026bb0f9cc568d374f2c", "start_type": "TTG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6321.744299999997, "isoelectric_point": 7.95754222869873 }, "id": "NKIECMJPJD_621", "locus": "GCA000828735_00606" }, { "type": "cds", "sequence": "contig_1", "start": 362399, "stop": 363352, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATTAACATATCGTGGAAGCGATAATGAACTTATTTCATCAACAACTAATATAGTAAAATCAAAAACTTCATATACATTTACTTTAAATAATTTAAAACATAATCGTGTTTATCATTTAGAAAAAGTAGAATTAGTTGATAATAATACAACAACTCCTTTCGCTAAATTAGATAATATAACTAATTCATTTATTGTAGAACGAACTGAAGCTGTAAATATTAGTGGTATTGAAGAGTTAGAAAATCGTAATCCAAATAATTTAAATAGCACAGTAGTTAAAATTAGTTTAATTGACACAGATAATGTATTAGATGCTAATGAAGTTGTAAATATTAGTTATAACAATAATAAAACAGTTCATGGAACTGTTAAAATGCAAAACAAAAATAAATATATTGAAGCAGTATTTAATGATTTAGTTTTAAATGAAGACACAGTTATTAAAAGTATTAATTTTGCTAATAAACCATCAAAAGCTGGCGAAAACATTGGTATTAATAAAACTGATAACAAGATTTATGATAATGTAATAAACACAAACAAATCATTAATAATTAACAATAATTTTAAATTGGTTGGTCCAACTGTAAATAGACAGATTACTAAAGAAGCAAATCAAAAAACAAATATTACGTTTGATTTAGATGTGAATATAAATCCAAAAATTGCCAATAATTTAAGATTTAAAGCTAAATTTAAAGCTACAAATGGTGAATTTGTTGAGACAAATATTGCTACTATTACATCAATTAGTAATAAACAAAAAATTAATTTTACATTGACTAATTTAAAAGCTAATCAAATGTATACTTTAAGTGAGCTTTATTACCTAACAAATCCTAATGATAATACGATTGTTAATAATAATAAAATTCAAAAAGAACCAAATGTTTCAAGAATGATTTTGGTTAATAAGGGATCAACATTAATTAGCAAGTTTGGTGAATGA", "aa": "MKLTYRGSDNELISSTTNIVKSKTSYTFTLNNLKHNRVYHLEKVELVDNNTTTPFAKLDNITNSFIVERTEAVNISGIEELENRNPNNLNSTVVKISLIDTDNVLDANEVVNISYNNNKTVHGTVKMQNKNKYIEAVFNDLVLNEDTVIKSINFANKPSKAGENIGINKTDNKIYDNVINTNKSLIINNNFKLVGPTVNRQITKEANQKTNITFDLDVNINPKIANNLRFKAKFKATNGEFVETNIATITSISNKQKINFTLTNLKANQMYTLSELYYLTNPNDNTIVNNNKIQKEPNVSRMILVNKGSTLISKFGE", "aa_hexdigest": "6a6355c955de20629d20948d12ee67da", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 35773.96520000001, "isoelectric_point": 9.24626522064209 }, "id": "NKIECMJPJD_622", "locus": "GCA000828735_00607" }, { "type": "cds", "sequence": "contig_1", "start": 363671, "stop": 364141, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGACTGCTTTAAATGTAGATAATAATGCAATTAGTACTGATACACAAACAATTCATGCAACTTTTTCTGGAGTTAATAACAAATATCAAAATCGTAAAGTTAAGTTGATTTATAAAGATAATAATGATCAAATTTATGAAACAAATTCAATCACTTTATCTAAGAAAAACAAAAATTATGATTTTACTTTAAATAATTTAATCGCGAACCGAAATTATATTTTTTACAAAATACAAATCACTGATATAGCTAATGATACAACTTATGTTGATTTGAAAAAAATCGATAGTTTAACTAATAGTTTTAAACGTAATCCATCATTAACAAGTATTGAATTAAATCAACCAACTAATGTTACACATAATAGTGCTATTTTAAATATCACACTAAAATCAGTTGATAAAGTATTTGAAGTTAACCAACAAGTTATAGTTTATATTACAAGTTCAGATGGACAAATACTTCATTGA", "aa": "MTALNVDNNAISTDTQTIHATFSGVNNKYQNRKVKLIYKDNNDQIYETNSITLSKKNKNYDFTLNNLIANRNYIFYKIQITDIANDTTYVDLKKIDSLTNSFKRNPSLTSIELNQPTNVTHNSAILNITLKSVDKVFEVNQQVIVYITSSDGQILH", "aa_hexdigest": "002115bf4eac2115f1cbdf0bedaea515", "start_type": "TTG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17810.75229999999, "isoelectric_point": 8.709630393981932 }, "id": "NKIECMJPJD_623", "locus": "GCA000828735_00608" }, { "type": "cds", "sequence": "contig_1", "start": 364205, "stop": 364396, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCGTTAAACGAACAAGTTGAGTATAAATTACTAAAAGTTGAATTTGCTCAAAAACCTAATTTAGCATATGAGAAATTAAATAACAATAACATAATTCAAAATAGTAATAATAATGCGAAGTTCACTACAAATATTGGACCAACAAAACTTATTAGTCTTTCATCAGTAAGTACAAATAACACATCTTGA", "aa": "MSLNEQVEYKLLKVEFAQKPNLAYEKLNNNNIIQNSNNNAKFTTNIGPTKLISLSSVSTNNTS", "aa_hexdigest": "578c1f6160a521d9e545ba8414ebe09b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7044.8191000000015, "isoelectric_point": 9.158330345153807 }, "id": "NKIECMJPJD_624", "locus": "GCA000828735_00609" }, { "type": "cds", "sequence": "contig_1", "start": 364499, "stop": 364894, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTATTAGAAGAAAGAAATAAAAAGAATTATCAATTTAATTTTAATAACTCATTAATTACTAATCGTGAATATAGATTCTATAAAGCAGAAATTACAAATGCAAAAAATTCAAATACTTTTATTGATTTAGAAACTAATAACGGTGTTAACAATAGTTTTACAATTAACCCAGGTAATACACATTTAATAATTGATAATAGTAAATTTGCTACAAATATCTCAGAAAATAGTGCAACAGTTTCAGTTAATATTACTTCGACTGATGGTGCTTTTGAGAATAATCAAGAAGTTGAAGCCAGATTTGAATCAATTGAAAATCCATCAGAAACTAAAGTATTTAATGGTGTATTAACTAATGTTTCAAATGACAAAAATAATGCTACAATCACTTGA", "aa": "MLLEERNKKNYQFNFNNSLITNREYRFYKAEITNAKNSNTFIDLETNNGVNNSFTINPGNTHLIIDNSKFATNISENSATVSVNITSTDGAFENNQEVEARFESIENPSETKVFNGVLTNVSNDKNNATIT", "aa_hexdigest": "804a3be2628bb42dbd3e83b049ede33a", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14766.85389999999, "isoelectric_point": 4.840829277038574 }, "id": "NKIECMJPJD_625", "locus": "GCA000828735_00610" }, { "type": "cds", "sequence": "contig_1", "start": 365039, "stop": 365707, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTAAAAAACCTAGATTAATTAAATTAGAAAGCCATAATATTGATTTTAATCTTGAAAATCTTTTAATGCCAAACAAAGAATTAGGAAAAATTAAAGCTACTATTTCTGATTTCAATGATAGTTATTTAAATAAAAGAATTAGGTTAATTTATGAATCTAATGATCACAAAGAATTATCAACTAATGAATTGGTTTTATCTAAGAATAAAGTTGAATATGAATTTGATGTTAAAAAGATTACATCAAATAGAAAATATACATTTAGAAGAATTGAAATTTACAATAATAAATTTGAAGTATTGGATAATGTCAATAATATTACTAATGAATTTATCTATTATAATGCACCTACAACTATTACAGTTCCAGGTGATTTAGCTTCAAATATTAGTAATAATAAGGCAACATTTAAATTAGGTTTGAATTCAATAGATAAAATTCTTGAAGATGGACAAGCAATTAGTGTTGTTTTAGCTAATAAAAATTATCCAAACGATACAAGCAAATACAAAACATATACAACATTTGTGAGAGAAACTAGAGATGATGGAATGCACGCAAATATTGTTCATGATTTAAATAATTTAGATGAACATAGTACATATTTTATTAAAGAAGTTAAATTTATAAATAAACCAAATAAAATTATTGAATATCCAAATTGA", "aa": "MFKKPRLIKLESHNIDFNLENLLMPNKELGKIKATISDFNDSYLNKRIRLIYESNDHKELSTNELVLSKNKVEYEFDVKKITSNRKYTFRRIEIYNNKFEVLDNVNNITNEFIYYNAPTTITVPGDLASNISNNKATFKLGLNSIDKILEDGQAISVVLANKNYPNDTSKYKTYTTFVRETRDDGMHANIVHDLNNLDEHSTYFIKEVKFINKPNKIIEYPN", "aa_hexdigest": "a82633fa6c44d04cadf25055bf160dc8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 25956.08220000003, "isoelectric_point": 8.476835060119633 }, "id": "NKIECMJPJD_626", "locus": "GCA000828735_00611" }, { "type": "cds", "sequence": "contig_1", "start": 365858, "stop": 365965, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAAATACAAATACACATTTTAAAATAACTTTTAGAAAAGACAATGATCAAAATCATCAAGAGAAAATTTATACTGGAAATTTATCTCTAGAAGGTAATGAATGA", "aa": "MLNTNTHFKITFRKDNDQNHQEKIYTGNLSLEGNE", "aa_hexdigest": "76d0886ec974787c3b3f548f024f925f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4151.4876, "isoelectric_point": 6.025694847106934 }, "id": "NKIECMJPJD_627", "locus": "GCA000828735_00612" }, { "type": "cds", "sequence": "contig_1", "start": 366263, "stop": 366544, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAAGTTCATTAACTTTGGATAATAACAAAAAAGACTATGAAGTTACAATTAGTAATTTAACTTATAATCGTGAATATACATTAAAAAATATTATTAAGATTAAAAATGGCCAAACACCTAAAGTATTAAATATTAGTAATAGTATAAATAAAAAATTTGTTGTAGACCCAAGTGTTACAAAAGTTTCAAACTTTAAAAATGACAAGTATGAAATAAATTATGCTAATTTAACTTTTACAAGAACTTTAGAATTTGATGTCAATAATTCTGATGAATGA", "aa": "MTSSLTLDNNKKDYEVTISNLTYNREYTLKNIIKIKNGQTPKVLNISNSINKKFVVDPSVTKVSNFKNDKYEINYANLTFTRTLEFDVNNSDE", "aa_hexdigest": "31c66768a2ae69d2ad8efeb86ff56c20", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10759.94219999999, "isoelectric_point": 8.830186271667483 }, "id": "NKIECMJPJD_628", "locus": "GCA000828735_00613" }, { "type": "cds", "sequence": "contig_1", "start": 366680, "stop": 366808, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAATTTAAGTTTAAATGAATTTAATCAAAAATATCAAATTAGATCAATTTATTTCTCAGGGGAAGTTAATCATAATTTAAATCAATTATTAAATAATGAAGTTCAAAATTCTGTTTATTTATGA", "aa": "MKNLSLNEFNQKYQIRSIYFSGEVNHNLNQLLNNEVQNSVYL", "aa_hexdigest": "1b5dfb9fc0f22c40362617e00bee561c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5017.544600000001, "isoelectric_point": 6.5197961807250975 }, "id": "NKIECMJPJD_629", "locus": "GCA000828735_00614" }, { "type": "cds", "sequence": "contig_1", "start": 366938, "stop": 367138, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAAGTTTAATGGTTTATTAAAAGATTTACAAAAATTCTCACCGCAAGGAAACAATAACCAAACAATTTTTGCTAAGTTACTTTTTGTTAATCTAAATAACAATAAACTTTATCAGACAGGTTTAGGAATTATTAAAGATGATAATAAAGTCAATAATTATGACAATTCTTCAAACTTCATTGTTCCAGCTAATGAATGA", "aa": "MKFNGLLKDLQKFSPQGNNNQTIFAKLLFVNLNNNKLYQTGLGIIKDDNKVNNYDNSSNFIVPANE", "aa_hexdigest": "2a59e1265cf8e9521182540edf8b96d2", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7491.364900000001, "isoelectric_point": 9.100760078430177 }, "id": "NKIECMJPJD_630", "locus": "GCA000828735_00615" }, { "type": "cds", "sequence": "contig_1", "start": 367596, "stop": 368264, "strand": "-", "frame": 1, "gene": "med", "product": "Conserved hypothetical ATP/GTP-binding protein", "db_xrefs": [ "COG:COG1744", "COG:T", "SO:0001217", "UniRef:UniRef50_Q9PQ10", "UniRef:UniRef90_Q9PQ10" ], "nt": "ATGAGTTTAGCAGTTAATGAAGCAATTAGTACAACTTCACACAAAGTAGCAATAATAGGCGTAGATACTGAACAAGAAAACGATAAGACAATTAATAAAGAAGCAAATACTTTTAAAAATACTAATATAAGCGGAAATAAAAACGGTGTTATTCGTTTTTCAATTGTTAAACGATTAGGAGTAGCAATTGAAAAATTACTAGAAAACGCTGTAAATGGTACTCAATTTCAAGGACATGTTAACAAAATTGGTGATGAAATTGATGTTCACGATAAATATAAATTAGGTGTAAACACTGTAGGTTCACTAGTTGATGGTGTAGTAGGAATCTCAGAATCTGCTTATCACTATTTAATTGATGCTTTTAATCTAGCACAAACAGATGAAACTAATAAAGTAAGTACATATGACCAATTAGTTCAAAAAATGATCCAAGATCCTTTATTTAAAAGTTTAAACCAAGATCCTAAAATTGATGGATATATAAACCCTAAAAATCTTGCTAACGCTAGGGGTAATGAAGGTGAATTAATAAATGGAAAAACAATTAGTCCAGAAGTAAATGGGGGAACCATTTATAAAGCAGCAAATGGTTCATATTATTACTATCCTGTTGCTAAATCAACATATACTGCACAAAATGCCTCAACTATTTTTAAAGAAATATGA", "aa": "MSLAVNEAISTTSHKVAIIGVDTEQENDKTINKEANTFKNTNISGNKNGVIRFSIVKRLGVAIEKLLENAVNGTQFQGHVNKIGDEIDVHDKYKLGVNTVGSLVDGVVGISESAYHYLIDAFNLAQTDETNKVSTYDQLVQKMIQDPLFKSLNQDPKIDGYINPKNLANARGNEGELINGKTISPEVNGGTIYKAANGSYYYYPVAKSTYTAQNASTIFKEI", "aa_hexdigest": "f707d00bfecb8ff83906053f721a0976", "start_type": "ATG", "rbs_motif": "AGxAG", "genes": [ "med" ], "truncated": "both", "pseudogene": { "start": 367440, "stop": 368564, "observations": { "mutation": [ 367598, 368402, 368465 ], "stop": [ 367598, 368402, 368465 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQ10", "query_cov": 1.0, "subject_cov": 0.41187384044526903, "identity": 0.991, "score": 428.0, "evalue": 7.68e-147, "gene_start": 1, "gene_end": 222, "reference_start": 266, "reference_end": 487, "reference_sequence": "MIKKHIKKIVIGALSTLAFITTTVVTTACAKHNNSVTKNNEPNVNVKNEISSSNSELNDSHYYWKAPKSDTSEGFQTIHKSMVEDGKRAFLLPGFSQSNYLQDAIKNAKFDKNAVGFLLDTVYNANNDPSLFDGANRVASVYFKVDDAAFLGGIAAAYMLNANQNIFATDGQLNWGGYVALNAKNTTNYLAGFALGIEWANKHLANKKVLQEGSSESKTWIKVNEVQASQSSAGGFDENNENAKKILKELISKKADLILPVAITQMSLAVNEAISTTSHKVAIIGVDTEQENDKTINKEANTFKNTNISGNKNGVIRFSIVKRLGVAIEKLLENAVNGAQFQGHVNKIGDEIDVHDKYKLGVNTVGGLVDGVVGISESAYHYLIDAFNLAQTDETNKVSTYDQLVQKMIQDPLFKSLNQDPKIDGYINPKNLANARGNEGELINGKTISPEVNGGTIYKAANGSYYYYPVAKSTYTAQNASTIFKEIWDKAKNDDDKRRLVGLILSFAGANVKDGGYSEISYDGMLGFYAKHGVKIPKL" }, "paralog": false, "identity": 0.9812834224598931, "score": 719.0, "evalue": 8.27e-264, "description": "Internal stop codon at 367598, 368402, 368465. Nonsense mutation around 367598, 368402, 368465.", "psc": { "uniref90_id": "UniRef90_Q9PQ10", "gene": "Med", "product": "Conserved hypothetical ATP/GTP-binding protein", "uniref50_id": "UniRef50_Q9PQ10", "cog_id": "COG1744", "cog_category": "T", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQ10", "UniRef:UniRef50_Q9PQ10", "COG:COG1744", "COG:T" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQ10", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQ10" ], "product": "Conserved hypothetical ATP/GTP-binding protein" } }, "id": "NKIECMJPJD_631", "locus": "GCA000828735_00616" }, { "type": "cds", "sequence": "contig_1", "start": 368463, "stop": 368816, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTCGAAGATGGAAAACGTGCATTTTTATTACCAGGGTTTTCACAAAGCAATTATCTACAAGACGCAATAAAAAACGCTAAATTTGATAAAAATGCTGTTGGTTTTTTATTAGATACAGTTTATAATGCAAATAATGATCCTTCTTTATTTGATGGTGCAAATCGTGTTGCAAGTGTTTATTTTAAAGTTGATGATGCTGCTTTTTTAGGTGGAATTGCTGCCGCTTATATGCTAAATGCAAACCAAAACATTTTTGCAACAGATGGCCAACTTAACTGGGGAGGATATGTTGCTTTAAATGCTAAAAACACTACTAATTATTTAGCAGGATTTGCCTTGGGTATTGAATGA", "aa": "MVEDGKRAFLLPGFSQSNYLQDAIKNAKFDKNAVGFLLDTVYNANNDPSLFDGANRVASVYFKVDDAAFLGGIAAAYMLNANQNIFATDGQLNWGGYVALNAKNTTNYLAGFALGIE", "aa_hexdigest": "9fdfdb1799e6fe29e4cbab56f882e960", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12613.953899999986, "isoelectric_point": 4.677076148986816 }, "id": "NKIECMJPJD_632", "locus": "GCA000828735_00617" }, { "type": "cds", "sequence": "contig_1", "start": 368868, "stop": 369059, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATAAAAAAACATATTAAAAAAATAGTAATTGGAGCTTTAAGTACACTAGCGTTCATCACAACAACAGTTGTAGCAACAGCTTGTGCAAAACACAATAATTCTGTTACTAAAAATAATGAACCAAATGTTAATGTTAAAAATGAAATAAGTTCTTCTAATAGTGAATTAAATGATTCACATTATTATTGA", "aa": "MIKKHIKKIVIGALSTLAFITTTVVATACAKHNNSVTKNNEPNVNVKNEISSSNSELNDSHYY", "aa_hexdigest": "cd84d166bb23d45187c5663627d86e5d", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6902.776100000001, "isoelectric_point": 9.252583122253416 }, "id": "NKIECMJPJD_633", "locus": "GCA000828735_00618" }, { "type": "cds", "sequence": "contig_1", "start": 369620, "stop": 370012, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF07198.17" ], "nt": "TTGTCTAATTCCATAATTGGTTTAACTTTATCTAAAATAGATAAAACCAATGAATATTCAAATACTGGTGATATTGTTATTAAAGTTAATTTAAAAAACAAAGCTAAAAGGAATCCGCAAATTATGGGTGTTTTTGTTGATGAAAAGCATGAATATCATTACATACAACCAACTATTAAAGATAAAGTTGCTTATTTTAATACATCAAAATTAGTAAATAATCATGATTATCGTTTAGTAAAAATTTTTGACAAAACTGATTATAATCACGTTTTAATTCAACAAAATGAGTTAGCGATTGAACATCAAATTATGATTAGTAAACCACCTAAAACAGCTTCTTTTATTAACCAAAATAATCAAAAAATATACCGAATAAATGTAGGAAAGTGA", "aa": "MSNSIIGLTLSKIDKTNEYSNTGDIVIKVNLKNKAKRNPQIMGVFVDEKHEYHYIQPTIKDKVAYFNTSKLVNNHDYRLVKIFDKTDYNHVLIQQNELAIEHQIMISKPPKTASFINQNNQKIYRINVGK", "aa_hexdigest": "b5d93b5a9bdb256d73d775356214f40e", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07198.17", "name": "DUF1410", "length": 56, "aa_cov": 0.4230769230769231, "hmm_cov": 1.0, "evalue": 7.587501208304705e-13, "score": 44.25861740112305, "start": 37, "stop": 91 } ], "seq_stats": { "molecular_weight": 15120.196299999987, "isoelectric_point": 9.547074699401854 }, "id": "NKIECMJPJD_634", "locus": "GCA000828735_00619" }, { "type": "cds", "sequence": "contig_1", "start": 370074, "stop": 370238, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF07198.17" ], "nt": "ATGGCTAAAGTAGATGTAGAAGGAAATATTGATTTTGATGTAAGCAAATTAGCAAATAATAACTTCTATGAATTAGTTAACATTTATAAAAATAACACGCATCCTTTAGTTAAAGTTATTAATCCAATTAATATCGCATATCATAATAAAACTATTAACATTTAA", "aa": "MAKVDVEGNIDFDVSKLANNNFYELVNIYKNNTHPLVKVINPINIAYHNKTINI", "aa_hexdigest": "52ae32b8b1693ddbb47df858967bc0ff", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07198.17", "name": "DUF1410", "length": 37, "aa_cov": 0.6666666666666666, "hmm_cov": 0.972972972972973, "evalue": 1.6948137019763272e-09, "score": 33.53208541870117, "start": 2, "stop": 37 } ], "seq_stats": { "molecular_weight": 6186.999900000001, "isoelectric_point": 6.700714683532715 }, "id": "NKIECMJPJD_635", "locus": "GCA000828735_00620" }, { "type": "cds", "sequence": "contig_1", "start": 370770, "stop": 370982, "strand": "+", "frame": 3, "gene": null, "product": "MAGa4850 family ICE element protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0011851DAE", "UniRef:UniRef90_UPI0011851DAE" ], "nt": "GTGTTGATTGAAGTTTTTATGGTTATTAAATCCTTTAAAAATAAAGAAATTAAAATCGTTGATTTAATAAATTTAGGATTTAAAAAAACTTCTATAAAAATGGCTATAAAAAGACTTATTGAAATAAAGGTTTTAGATGGTAAGTTTTATAAAAAATATAAGCTTGAAAAATTAAAAAAAATAGTACTAAAAAACTCTAAAGAAAATTACTGA", "aa": "MLIEVFMVIKSFKNKEIKIVDLINLGFKKTSIKMAIKRLIEIKVLDGKFYKKYKLEKLKKIVLKNSKENY", "aa_hexdigest": "491828d1c265a2cba8e6c4e625ccafb6", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 370773, "stop": 371051, "observations": { "mutation": [ 370980 ], "stop": [ 370980 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0011851DAE", "query_cov": 0.9857142857142858, "subject_cov": 0.75, "identity": 0.986, "score": 123.0, "evalue": 2.84e-35, "gene_start": 2, "gene_end": 70, "reference_start": 1, "reference_end": 69, "reference_sequence": "MIEVFMVIKSFKNKEIKIVDLINLGFKKTSIKMAIKRLIEIKVLDGKFYKKYKLEKLKKIVLKNSKENYWILNGYDALKIFLLNGLSNAVYM" }, "paralog": false, "identity": 0.9782608695652174, "score": 174.0, "evalue": 6.48e-58, "description": "Internal stop codon at 370980. Nonsense mutation around 370980.", "psc": { "uniref90_id": "UniRef90_UPI0011851DAE", "product": "MAGa4850 family ICE element protein", "uniref50_id": "UniRef50_UPI0011851DAE", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0011851DAE", "UniRef:UniRef50_UPI0011851DAE" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0011851DAE", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0011851DAE" ], "product": "MAGa4850 family ICE element protein" } }, "id": "NKIECMJPJD_636", "locus": "GCA000828735_00621" }, { "type": "cds", "sequence": "contig_1", "start": 372392, "stop": 372580, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTACCAAGAATTCATTAAATCTTCAAAAGGTATTTTAGATGAAGTTTTAAACAAAAAAAATATTTCATATTCTCGTATTTCTGATGAAAATAAAGAAAATATTGAAATAATAAAAAAATTCTTATTAAATTTTGACAATGATTATTCAAAGCTCTTCAATTCTTATCAAAACCAAATTAAGGATTAA", "aa": "MYQEFIKSSKGILDEVLNKKNISYSRISDENKENIEIIKKFLLNFDNDYSKLFNSYQNQIKD", "aa_hexdigest": "c8e0da68f756fd0f10b2789f56da8896", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7455.323299999999, "isoelectric_point": 6.209171104431152 }, "id": "NKIECMJPJD_637", "locus": "GCA000828735_00622" }, { "type": "cds", "sequence": "contig_1", "start": 373389, "stop": 373538, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAGAACTTTATTATTCAAAAAAGCAAAACTTAATAATTAGTGATCCCAAGAAGGAAATTATCAAATTTACAGGAGGCTTGTTAACTGAAAACGATTACAAAATTTATGTAATTGATTTCTCTGAGCCTAAATTGTCTCTAAAATGA", "aa": "MAELYYSKKQNLIISDPKKEIIKFTGGLLTENDYKIYVIDFSEPKLSLK", "aa_hexdigest": "4df5ecb6b742fe78231a3504be397b2a", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5726.632800000001, "isoelectric_point": 7.942392158508301 }, "id": "NKIECMJPJD_638", "locus": "GCA000828735_00623" }, { "type": "cds", "sequence": "contig_1", "start": 374225, "stop": 374521, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATTTTTAAAGTTAATGAATATTACATATTTTTAGAAGCAAAACACATAAAAGAATCAGGAGGTGCTCAGGATAAACAAATTAAGGAATTAATTGAATTATTAAATATCGATTTACCTAAAAATATTTTTTTAATTTCATATCTTGATGGAGTATATTCTAATTATTTACTAGATCTAACAACCCAAGAAATAGTTAATCCTGAGGTTATAAAACAACAAAATTTAAAATCGAAGATTCTTCAACAAAGATATGAAATTATTTTGAGTCTAAAAAAGAATAAAAGATGTTTTTGA", "aa": "MIFKVNEYYIFLEAKHIKESGGAQDKQIKELIELLNIDLPKNIFLISYLDGVYSNYLLDLTTQEIVNPEVIKQQNLKSKILQQRYEIILSLKKNKRCF", "aa_hexdigest": "c1b111d02bde4c2049a6cd943ae411ad", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11593.464699999988, "isoelectric_point": 8.625112342834473 }, "id": "NKIECMJPJD_639", "locus": "GCA000828735_00624" }, { "type": "cds", "sequence": "contig_1", "start": 374735, "stop": 375103, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATTAAAGTCGAATTTAGAATATTTAATCAAATCAGTCGAAAATAAAGAATCTTTAGATTCTAGATATATGTCCCTTAAAATGCACGAATCGTTAAACGAAATGGTTGAAACAACCAAGCAAATAAAAGAATTGTTAATAACAATTAAGACTTTAAATAAAAATAACATGAGTTCAGAAAAAATATATACTAAATTAAAAGAATTGGTTTTAAATGAATTAGGAAATAAAAGTGAATTTAATTGTTTTATTAATGCTTGTGACTCCGCCACTTCCACCATAAAATCAAATCCAGAGACATTAAAATCAATTGTTAATTTATATTTACAATATAGAGATATAAGTGAATATACTCCTAAAGAATGA", "aa": "MKLKSNLEYLIKSVENKESLDSRYMSLKMHESLNEMVETTKQIKELLITIKTLNKNNMSSEKIYTKLKELVLNELGNKSEFNCFINACDSATSTIKSNPETLKSIVNLYLQYRDISEYTPKE", "aa_hexdigest": "8d7f48f3a904954bf12a69bfa85758c5", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14180.20199999998, "isoelectric_point": 7.68743953704834 }, "id": "NKIECMJPJD_640", "locus": "GCA000828735_00625" }, { "type": "cds", "sequence": "contig_1", "start": 375375, "stop": 375893, "strand": "-", "frame": 1, "gene": null, "product": "site-specific DNA-methyltransferase (adenine-specific)", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A097ST28", "UniRef:UniRef90_A0A097ST28" ], "nt": "TTGCTAAAAATTCCTAGTATAAATAATGCGATTATAATAACTAATCCGCCATATTTAGCTAAGCATTCAGCAACAAGAAAAAAAATTGATTTATCAAAATATTTTAATAATAGTATATATGATGATTTGTATTTAATAGCATTAGAGAGAATGATAGAAGCGCAAAAATATATAGTCGCAATTATTCCTGAATCTTTTGTAAATTCTTCGTTTAAAAATAAAGATCTCTTATCATCGATTACCATTCTTGAAGAAAATCCTTTTGATGATACTCACACACCAATTTGTGTTGCTTGTTTTGATGGAATACCAAAGACTTATGACAAAATTAAAATTTATAAAAACAATAATTATATAAATACATTGTCGTTTCTTCAATCCTTAAAAATATCTCCCAAAAAAACTATGAAAATTTTATTCAATGATTTGCACGGTTGGCTAGGAATAAGAGCTATTGACGGCACAGATGATAAAAAATCAATTCATTTTGATTTTAAAGAAAAAATACCATATAATTGA", "aa": "MLKIPSINNAIIITNPPYLAKHSATRKKIDLSKYFNNSIYDDLYLIALERMIEAQKYIVAIIPESFVNSSFKNKDLLSSITILEENPFDDTHTPICVACFDGIPKTYDKIKIYKNNNYINTLSFLQSLKISPKKTMKILFNDLHGWLGIRAIDGTDDKKSIHFDFKEKIPYN", "aa_hexdigest": "3591867280bb50b7a4e8e58f56d72508", "start_type": "TTG", "rbs_motif": "3Base/5BMM", "genes": [], "truncated": "both", "pseudogene": { "start": 375132, "stop": 376130, "observations": { "mutation": [ 375377, 375905, 375911 ], "stop": [ 375377, 375905, 375911 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A097ST28", "query_cov": 1.0, "subject_cov": 0.5180722891566265, "identity": 0.924, "score": 329.0, "evalue": 1.17e-111, "gene_start": 1, "gene_end": 172, "reference_start": 80, "reference_end": 251, "reference_sequence": "MKKDINANEQKIKLGQFFTRQKFWLKPQIVDFILNSKCSIAYDPFAGAGNLINISKLYGINKIIGLDIDNHLGWEWNDSLLKIPSINNAIIITNPPYLAKHSATRKKIDLSKYFNNSIYNDLYLIALEKMIEAQQYIVAIIPESFINSSFKNKNLLSSITILEENPFDDTDTPICVACFDGIPKTYDKIKIYKNNNFINTLAFLYSLKISPKKTIKILFNDLHGWLGIRAIDGADDKKLIHFDFKEKIPYNWETKINHSSRHYTLVNIDIKQKNRDSFINKCNSILQELRNKSTDTILTPFMGNTKSGIRRRRMDFKLARAIMEQASDYFDK" }, "paralog": false, "identity": 0.9216867469879518, "score": 618.0, "evalue": 6.16e-228, "description": "Internal stop codon at 375377, 375905, 375911. Nonsense mutation around 375377, 375905, 375911.", "psc": { "uniref90_id": "UniRef90_A0A097ST28", "product": "site-specific DNA-methyltransferase (adenine-specific)", "uniref50_id": "UniRef50_A0A097ST28", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A097ST28", "UniRef:UniRef50_A0A097ST28" ] }, "pscc": { "uniref50_id": "UniRef50_A0A097ST28", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A097ST28" ], "product": "site-specific DNA-methyltransferase (adenine-specific)" } }, "id": "NKIECMJPJD_641", "locus": "GCA000828735_00626" }, { "type": "cds", "sequence": "contig_1", "start": 375909, "stop": 376130, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGAAAGATATCAAAGCAAATGAACAAAAAATAAAACTAGGCCAATTTTTTACAAGACAAAAATTTTGGTTAAAACCACAAATTATTGATTTTATATTAAATTCTAAGTGTTCAATTGCTTATGATCCCTTTGCAGGCGCTGGCAATTTGATTAATATATCAAAATTATATGGAATTAATAAAATCATTGGACTAGATATTGATAATCATCTAGGTTGA", "aa": "MKKDIKANEQKIKLGQFFTRQKFWLKPQIIDFILNSKCSIAYDPFAGAGNLINISKLYGINKIIGLDIDNHLG", "aa_hexdigest": "7e28c452fc9acb14fe752bee826be693", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8310.690899999994, "isoelectric_point": 9.56325626373291 }, "id": "NKIECMJPJD_642", "locus": "GCA000828735_00627" }, { "type": "cds", "sequence": "contig_1", "start": 377288, "stop": 377437, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00145.23" ], "nt": "ATGCTATTAATGGGCTACGACCAACAAGATTATGAAAGAATTAAGAATTTCTGTGATAGTCAAATTTATAAGTTTGCCGGAAATTCTATTGTTGTTAATGTATTACAAGAAATAATTAAACACATTCTTAAACTAAATAAAAAGGAGTAA", "aa": "MLLMGYDQQDYERIKNFCDSQIYKFAGNSIVVNVLQEIIKHILKLNKKE", "aa_hexdigest": "b708815929089471c4cb9ba0fbcad629", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00145.23", "name": "DNA_methylase", "length": 43, "aa_cov": 0.8775510204081632, "hmm_cov": 0.9302325581395349, "evalue": 2.438197518679597e-06, "score": 22.703420639038086, "start": 2, "stop": 44 } ], "seq_stats": { "molecular_weight": 5832.769299999999, "isoelectric_point": 7.867608833312988 }, "id": "NKIECMJPJD_643", "locus": "GCA000828735_00628" }, { "type": "cds", "sequence": "contig_1", "start": 378575, "stop": 378820, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00307F40E4", "UniRef:UniRef90_UPI00307F40E4" ], "nt": "ATGTTTATACAAAAAAATGAAAGTAAGCTACTAACTAATGCGGATTTTGAATTGTTTGATATTAGAAGTTTAGAAAAACTTATTAAATTTGATAATTCTAAACACAAAAACCAAAATAATAAAAACCCAAAAGTTTTAAATGATAACAATTTAACTTATTTAGAAGATGTTGAAAATCCTGAGGATTTATCCCAAGAACAAAAAATTCAAAGAATGAAGATAGTCTTTGTAAAACTAGATCTTTGA", "aa": "MFIQKNESKLLTNADFELFDIRSLEKLIKFDNSKHKNQNNKNPKVLNDNNLTYLEDVENPEDLSQEQKIQRMKIVFVKLDL", "aa_hexdigest": "0d3e63c9a5be96d45b9365c75fb95c9c", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 378308, "stop": 378820, "observations": { "mutation": [ 378424, 378475, 378577 ], "stop": [ 378424, 378475, 378577 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00307F40E4", "query_cov": 1.0, "subject_cov": 0.4764705882352941, "identity": 1.0, "score": 155.0, "evalue": 1.1e-46, "gene_start": 1, "gene_end": 81, "reference_start": 1, "reference_end": 81, "reference_sequence": "MFIQKNESKLLTNADFELFDIRSLEKLIKFDNSKHKNQNNKNPKVLNDNNLTYLEDVENPEDLSQEQKIQRMKIVFVKLDLWNNYLNLKNDYKRTLIPHKKNQIKEKIEETLSLMWDQYVAKDLESDLLNILWSKAKEHKIPNYHDLNVDILLYLLDPPEDISKLIEKFN" }, "paralog": false, "identity": 0.9764705882352941, "score": 326.0, "evalue": 9.57e-117, "description": "Internal stop codon at 378424, 378475, 378577. Nonsense mutation around 378424, 378475, 378577.", "psc": { "uniref90_id": "UniRef90_UPI00307F40E4", "uniref50_id": "UniRef50_UPI00307F40E4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00307F40E4", "UniRef:UniRef50_UPI00307F40E4" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00307F40E4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00307F40E4" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 9666.9108, "isoelectric_point": 5.781287193298339 }, "id": "NKIECMJPJD_644", "locus": "GCA000828735_00629" }, { "type": "cds", "sequence": "contig_1", "start": 379442, "stop": 379690, "strand": "+", "frame": 2, "gene": null, "product": "DNA cytosine methyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_H3NC05", "UniRef:UniRef50_UPI001DF917AA", "UniRef:UniRef90_UPI002630C601" ], "nt": "ATGAGAGTACTTAATTTTGGAATAATAGATATTGGAAACGGTAAATATAGGTATTTAACAGAAAGAGAATGCCTAAGACTTATAGGTTTTGATGATAGCGATATAGATAAATTAGAGGAAGCACATCCAAGAAGAAAAAACTATATCTCAAGTAAACTATATAAGCAGACTGGAAATTCTATTATGGTTGATATTTTAACATGCATTATTATTTCAATAATGCATGTTAGTGCAAACTTATTATTGTAA", "aa": "MRVLNFGIIDIGNGKYRYLTERECLRLIGFDDSDIDKLEEAHPRRKNYISSKLYKQTGNSIMVDILTCIIISIMHVSANLLL", "aa_hexdigest": "752248a480c19dc54822276f6cee31b4", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_H3NC05", "query_cov": 0.8536585365853658, "subject_cov": 0.8045977011494253, "identity": 0.8, "score": 112.0, "evalue": 7.5e-31, "valid": false, "uniref50_id": "UniRef50_H3NC05" }, "pscc": { "uniref50_id": "UniRef50_H3NC05", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_H3NC05" ] }, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 379374, "stop": 379663, "observations": { "deletion": [ 379416 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI002630C601", "query_cov": 0.8536585365853658, "subject_cov": 0.6730769230769231, "identity": 0.8290000000000001, "score": 119.0, "evalue": 1.57e-33, "gene_start": 1, "gene_end": 70, "reference_start": 24, "reference_end": 93, "reference_sequence": "IDNSFRGRLKVIKDFSYTISTKQMRVPNSGIIDIGNGQYRYLTERECLRLMGFDDSDIDKLEKVHPRRKNCISSKLYKQAGNSIVVDVLMVIIKEIYENDLTKK" }, "paralog": false, "identity": 0.8333333333333334, "score": 155.0, "evalue": 3.35e-50, "description": "Frameshift due to deletion around 379416.", "psc": { "uniref90_id": "UniRef90_UPI002630C601", "product": "DNA cytosine methyltransferase", "uniref50_id": "UniRef50_UPI001DF917AA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI002630C601", "UniRef:UniRef50_UPI001DF917AA" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001DF917AA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001DF917AA" ], "product": "DNA cytosine methyltransferase" } }, "id": "NKIECMJPJD_645", "locus": "GCA000828735_00630" }, { "type": "cds", "sequence": "contig_1", "start": 379726, "stop": 380685, "strand": "+", "frame": 1, "gene": null, "product": "Rad50/SbcC-type AAA domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A134AXN1", "UniRef:UniRef90_A0A134AXN1" ], "nt": "ATGGATATTCTAAAATGTAAATTAAAGAATTGTTATGGAATAGACGAGTTTAACCATGAATTTGATTTTACAAATACAAATGTGATTACAATATATGCAAAAAATGGATTGATGAAAACTTCTTTTGCAAAAACTTTTAAAAAAATTCAAGATGGTAAAGCTGACGAAATACGTGATGAAATATTCGATATAAAAGCAGAAGTTAATGTGAGTATAGATGGACAGGATATAAGAAAAGAGCAAGTGTTTGTAATAAAAAGCTTTGAAAATTATTATGAGTCTAGTAGTGTAGCGGATTTATTAGTAGACGAAAAAATAAAAAAATCTATCACAACTTTATTAAATCAAAAAAATAATTTTTTAAAAAAGTTAGTGCAATATTCAGGAATGAAAATTGAAAAAACACAACAAGGAAGAAAAATTTATGAATTAGAGCCTACAATAGTTTCTGACATGAATTTAAGTGAAAAAAGTTTTTTATTAAGCTTGAAAGAATTAGATGAAGCTGAAAATGAAACGTATTTACCTAATGTTAAATACTCTACAATTTTTGATGATAGTGTAATTAAAAAAATAAAAGACGATAGTTTTCAAAAAAAAATAAAAGAATTTTGTGAAGCTGCTGAAACTATATATAGTAGTTATACTTTTTTTGAAAAAGGTAAATTCACATTTCCAAAATTGAAAAATGTTGCAAAAAAATTATATTCAGATAATTTTTTTGTAAGAGATAATAAACTTAATTTAAATAATAGCATAGTAATTAGTAATAATGATGAATTACAAGAGAAAATATCTGAAATAGAAGGTAAAATTAAAGATATTCCAGAATTTCAACAGATAGAAAACCTACTAAGTGATACAAAAGGTATATTATTAAGGGATACAATAGAAAATAACCCTGAACTAATAGAGTTTTTAAAAGAAGAAAATTTAAAGAATTTGAAAAAAGAATTATGA", "aa": "MDILKCKLKNCYGIDEFNHEFDFTNTNVITIYAKNGLMKTSFAKTFKKIQDGKADEIRDEIFDIKAEVNVSIDGQDIRKEQVFVIKSFENYYESSSVADLLVDEKIKKSITTLLNQKNNFLKKLVQYSGMKIEKTQQGRKIYELEPTIVSDMNLSEKSFLLSLKELDEAENETYLPNVKYSTIFDDSVIKKIKDDSFQKKIKEFCEAAETIYSSYTFFEKGKFTFPKLKNVAKKLYSDNFFVRDNKLNLNNSIVISNNDELQEKISEIEGKIKDIPEFQQIENLLSDTKGILLRDTIENNPELIEFLKEENLKNLKKEL", "aa_hexdigest": "1f23810b1d8d577fc91eb2e3ef950021", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 379726, "stop": 380988, "observations": { "mutation": [ 380683 ], "stop": [ 380683 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A134AXN1", "query_cov": 1.0, "subject_cov": 0.7088888888888889, "identity": 0.937, "score": 566.0, "evalue": 7.73e-201, "gene_start": 1, "gene_end": 319, "reference_start": 1, "reference_end": 319, "reference_sequence": "MDILECKLKNCYGIDEFNHEFDFTNTNVITVYAKNGLMKTSFAKTFKKIQDGKADEIRDEIFDIKAEVNVSVDGQDIRKEQVFVIKSFENYYESSSVADLLVDEKTKKSITTLLKQKNNFLKKLVQYSGLKIEKTQQGRKIYELEPTIVSDMNLSEKSFLLSLKELGEVENKTYLPNVKYSTIFDNSVIKKIKDSSFQNKIKEFCEAAETIYSSYTFFEKGKFTFPKLKNVAKNLDSDNFFVRDNKLNLNNSIVIGNNDELQEKLYEIEGKIKDIPEFQQIEKLLSDTKGILLRDTIENNPELIEFLKIENLDNLKKELWTSYIKKEKKSFDDLLEIYKELEENVKNLNIDNTLWNRSLEIFEDRFTVPYKMKISNLKGAIIGENLPRVEFIFEKGENQASLDRSNLEKLDVLSQGEKRSLYLLNIIFDIEKIRKDGFESISWTHFSRDG" }, "paralog": false, "identity": 0.9285714285714286, "score": 769.0, "evalue": 3.24e-283, "description": "Internal stop codon at 380683. Nonsense mutation around 380683.", "psc": { "uniref90_id": "UniRef90_A0A134AXN1", "product": "Rad50/SbcC-type AAA domain-containing protein", "uniref50_id": "UniRef50_A0A134AXN1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A134AXN1", "UniRef:UniRef50_A0A134AXN1" ] }, "pscc": { "uniref50_id": "UniRef50_A0A134AXN1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A134AXN1" ], "product": "Rad50/SbcC-type AAA domain-containing protein" } }, "id": "NKIECMJPJD_646", "locus": "GCA000828735_00631" }, { "type": "cds", "sequence": "contig_1", "start": 380839, "stop": 381291, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAATATCAAATTTAAAGGGAGCTATAATAGGAGAAAACCTACCACGTATGGAGTTTATATTTGAAAAGGGTGAAAATCACGAAAGTTTAGATAGATCAAATTTAGAAGAATTAGACGTATTAAGTCAAGGAGAGAAAAGATCTTTATATTTGTTAAATATAATATTTGACATAGAAAAAATAAAAAAAGAAAATAATGAAGTCCTATTTATAGTAGACGATATAGCAGATTCTTTTGATTATAAAAATAAGTATGCTATTGTAGAATATTTGTATGAAATGGCAACAGTTGACAATTTTAGAATGATAATTTTATCTCATAATTTTGATTTTTATAGAACAATTTCTTCTAGACTAAATATACCTCGTACATCTAGATTTATCGTTGAAAACAAACATGATTTGAATTTGATAGAAGAAAAATATCAAAAACAACCTTTTATAACATGA", "aa": "MKISNLKGAIIGENLPRMEFIFEKGENHESLDRSNLEELDVLSQGEKRSLYLLNIIFDIEKIKKENNEVLFIVDDIADSFDYKNKYAIVEYLYEMATVDNFRMIILSHNFDFYRTISSRLNIPRTSRFIVENKHDLNLIEEKYQKQPFIT", "aa_hexdigest": "b075478b925a809f501ce893fec39693", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17848.177999999996, "isoelectric_point": 5.152818489074705 }, "id": "NKIECMJPJD_647", "locus": "GCA000828735_00632" }, { "type": "cds", "sequence": "contig_1", "start": 381643, "stop": 381939, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAGACAAAATTAACAATTACAAAGGGATGCTCAATTGGAAAGAGAAAAATAATTTAAAAACAGGAGATAAAGTAAAATTTCTAAATCATGTATATAGAAGTAATAATATGACCATGGGATTATTTGGTGGATATAAGCAGATTGCGAATGAAGAACAAATTAATATAGTTAATGAAGTTAATATAATGACTCCAGAGAATATTCATATAAACTCTTTCATGTATGAACCTATAATGGATATGGATATTAACGAATTATTGAATTTATATGCGAAACTTAAAGAGTTAATATAG", "aa": "MLDKINNYKGMLNWKEKNNLKTGDKVKFLNHVYRSNNMTMGLFGGYKQIANEEQINIVNEVNIMTPENIHINSFMYEPIMDMDINELLNLYAKLKELI", "aa_hexdigest": "80a5dda744ff515525461c9bdaa13553", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11557.314499999993, "isoelectric_point": 6.079691886901855 }, "id": "NKIECMJPJD_648", "locus": "GCA000828735_00633" }, { "type": "cds", "sequence": "contig_1", "start": 382022, "stop": 382261, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A017H7K2", "UniRef:UniRef50_A0A930A405", "UniRef:UniRef90_UPI001D158681", "UniRef:UniRef90_UPI0023F10613" ], "nt": "ATGCAAGTAAATGATTTTAAGAATATACAAGAGGCTATAAAGTATGAAGTGTTACAAGATGAAAAAGAATATTTAAAACTCTTAAAAGCTATAGGTAATAATCAGAAATATGATTTTTCTAGCCAACTAAGTATATATAACAAGGAGCCTGAAGTTAGAGCTTGTGCAACTTTTGATATGTGGAAGAAATATTTTGGCAGAGTTGTTATGAGGGGGTGCAAAAGGGTATTCCAATTTTAG", "aa": "MQVNDFKNIQEAIKYEVLQDEKEYLKLLKAIGNNQKYDFSSQLSIYNKEPEVRACATFDMWKKYFGRVVMRGCKRVFQF", "aa_hexdigest": "ad2064cbc56dd1221120f67de7dcf030", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "psc": { "uniref90_id": "UniRef90_UPI001D158681", "query_cov": 0.8481012658227848, "subject_cov": 0.8170731707317073, "identity": 0.94, "score": 129.0, "evalue": 7.88e-38, "valid": true, "uniref50_id": "UniRef50_A0A017H7K2" }, "pscc": { "uniref50_id": "UniRef50_A0A017H7K2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A017H7K2" ] }, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 382022, "stop": 382509, "observations": { "deletion": [ 382235 ], "stop": [ 382415 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0023F10613", "query_cov": 0.9620253164556962, "subject_cov": 0.4, "identity": 0.9209999999999999, "score": 140.0, "evalue": 7.61e-41, "gene_start": 1, "gene_end": 76, "reference_start": 1, "reference_end": 76, "reference_sequence": "MQVNDFKNIQEAIKYEVLQDEKEYLKLLKVIGNNQKYDFSSQLSIYNKEPEARACAIFDMWKKYFGRVVMRGQKGIPILVGSDVNQRISYIFDISQTTSMDRNINEVSLWQFDHENHNEALKEIINNSSFEASDSLNENIFSLSRIYGDEYINLALADLRIDIEDRLSFEKCTDLPIVRFWSNFWERYSF" }, "paralog": false, "identity": 0.9135802469135802, "score": 283.0, "evalue": 1.34e-99, "description": "Internal stop codon at 382415. Frameshift due to deletion around 382235.", "psc": { "uniref90_id": "UniRef90_UPI0023F10613", "uniref50_id": "UniRef50_A0A930A405", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0023F10613", "UniRef:UniRef50_A0A930A405" ] }, "pscc": { "uniref50_id": "UniRef50_A0A930A405", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A930A405" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 9511.916299999999, "isoelectric_point": 9.144663047790527 }, "id": "NKIECMJPJD_649", "locus": "GCA000828735_00634" }, { "type": "cds", "sequence": "contig_1", "start": 382237, "stop": 382416, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_H3NC03", "UniRef:UniRef90_H3NC03" ], "nt": "GTGCAAAAGGGTATTCCAATTTTAGTTGGAAGTGATGTAAATCAAAGAATTTTATATATTTTTGATATTAGTCAGACCACATCAATGAATAAGAATATTAATGAGGTTAGTTTATGTCAGTTTGACCACGAAAACCATAACGAGGCTTTAAAAGAAATAATAAGAGATTTTTCATTTTAA", "aa": "MQKGIPILVGSDVNQRILYIFDISQTTSMNKNINEVSLCQFDHENHNEALKEIIRDFSF", "aa_hexdigest": "a9a32dc57f81f4bf740a5b84b308fe8e", "start_type": "GTG", "rbs_motif": "3Base/5BMM", "genes": [], "truncated": "both", "pseudogene": { "start": 382070, "stop": 382458, "observations": { "deletion": [ 382235 ], "stop": [ 382415 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_H3NC03", "query_cov": 0.9830508474576272, "subject_cov": 0.453125, "identity": 0.845, "score": 99.8, "evalue": 9.86e-26, "gene_start": 2, "gene_end": 59, "reference_start": 57, "reference_end": 114, "reference_sequence": "MLQDEKEYLKLLKVIGNNQKYDFYSQLSIYNKEPEDRDYATFDMWKKYFGRVVMRGQKSIPILVGSDINQRVSYIFDISQTTSMDRNINEVSLWQFDHENHNEDLKEIIRDSSFEASDSLNENIFSLS" }, "paralog": false, "identity": 0.8682170542635659, "score": 212.0, "evalue": 1e-72, "description": "Internal stop codon at 382415. Frameshift due to deletion around 382235.", "psc": { "uniref90_id": "UniRef90_H3NC03", "uniref50_id": "UniRef50_H3NC03", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_H3NC03", "UniRef:UniRef50_H3NC03" ] }, "pscc": { "uniref50_id": "UniRef50_H3NC03", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_H3NC03" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 6855.697699999997, "isoelectric_point": 5.0413003921508786 }, "id": "NKIECMJPJD_650", "locus": "GCA000828735_00635" }, { "type": "cds", "sequence": "contig_1", "start": 382621, "stop": 382833, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATATAGAGAATTTAAAAAATAATTTTTCTAGGATTAATACAATTTCTTTAGAACAGATAGGTCTTGTAATATCAAGGGTTAGCGAAGAGATTATAGATAGCACAATAGAAAAATCTAAAGAAATAGATCGTGCTAGGTTGCTTACAGAAAGGGCCACTGCCGACTATAATAGATATATAGAAAATACAAATGAAGATAGAGGAGGTTAA", "aa": "MDIENLKNNFSRINTISLEQIGLVISRVSEEIIDSTIEKSKEIDRARLLTERATADYNRYIENTNEDRGG", "aa_hexdigest": "4c0524ceca7a4588d7ed2e2224cc7323", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8072.855599999996, "isoelectric_point": 4.732835197448731 }, "id": "NKIECMJPJD_651", "locus": "GCA000828735_00636" }, { "type": "cds", "sequence": "contig_1", "start": 382892, "stop": 383059, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAGCGACAAAAGAAATAGCTATGAAGATCGAAGAAAAAACCTTGGACATGATGGGGAATGATCTGGAGTTTATGGAGAAATTTCCAAATCCGACTTATGCAGTTCTAAGACTGTCTTACCTAGTAGGGAGTAAGAGTATGGAGAATTGGAAGAAACTTCAGTAA", "aa": "MEATKEIAMKIEEKTLDMMGNDLEFMEKFPNPTYAVLRLSYLVGSKSMENWKKLQ", "aa_hexdigest": "a7878714ee9b0b7087cc7016de477baa", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6459.529900000001, "isoelectric_point": 5.285935401916503 }, "id": "NKIECMJPJD_652", "locus": "GCA000828735_00637" }, { "type": "cds", "sequence": "contig_1", "start": 383197, "stop": 383316, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCAAGGATAGATGAACAATTTGATGTCAACAGTGAAGAAAATGGCAATCAAGGAATACATGGAAGCTTAGAAAATGAAATAAGCCAAGAAAAGGAAGTTGATGAAGCTTTTTTTTGA", "aa": "MPRIDEQFDVNSEENGNQGIHGSLENEISQEKEVDEAFF", "aa_hexdigest": "ae5ee151a3d02b2d7963f6a4cfb7aad3", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4482.6735, "isoelectric_point": 4.0500284194946286 }, "id": "NKIECMJPJD_653", "locus": "GCA000828735_00638" }, { "type": "cds", "sequence": "contig_1", "start": 383300, "stop": 383524, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGCTTTTTTTTGATGGCAAAAACATTGAAAATAGAAAAGATTATTGGATTGTAGAATTTAATGAAAACCACGAATTAGTTCTTGATTATAGTGGACGGATAGTAACTAAAGATTTGATTAATGTCCTAAGACAAAAGGATATAGATGTTAAAGTCCATAATCAAACTCATGGAGAAAATGAATTTGGAAAAATGACCGATGATTATATCGAATATTTCTAA", "aa": "MKLFFDGKNIENRKDYWIVEFNENHELVLDYSGRIVTKDLINVLRQKDIDVKVHNQTHGENEFGKMTDDYIEYF", "aa_hexdigest": "31d126052ecb2322fc2d6293638b7b6c", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8969.9477, "isoelectric_point": 5.0169164657592775 }, "id": "NKIECMJPJD_654", "locus": "GCA000828735_00639" }, { "type": "cds", "sequence": "contig_1", "start": 383796, "stop": 383954, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAATATATGGGAGGTATGATTAGAGGTTCGGAAGTAATTCCATATAGAAACGATTCATACCTTGAAGAAATCTTTGAAAATTTAAGTCAAACATCAGAAAAACTGGCAGTAAAAGATTATGATCTTAAAAAAAAAAGAACAAAAACACTATTTTGA", "aa": "MEYMGGMIRGSEVIPYRNDSYLEEIFENLSQTSEKLAVKDYDLKKKRTKTLF", "aa_hexdigest": "f3283739ad5b4c343c9c1c8c8fcc37a1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6167.005900000001, "isoelectric_point": 6.18194522857666 }, "id": "NKIECMJPJD_655", "locus": "GCA000828735_00640" }, { "type": "cds", "sequence": "contig_1", "start": 384218, "stop": 384499, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_045470449.1", "SO:0001217", "UniParc:UPI0005EEBE22", "UniRef:UniRef100_UPI0005EEBE22", "UniRef:UniRef50_A0AAC9X6Z6", "UniRef:UniRef90_UPI0005EEBE22" ], "nt": "TTGATTGAATCGTACCAAAGTTACATTAAAAATCACAAACATTATTTAAAGATAAAAGAATTAGATGATGCTGAAATTACAATTTTAGTATGTTATATTAATTTAAATGAACAAAAAGATTTTTATTTTTATGGTGCTAGTTCAAATTCTACTTTAGATGAAAAAATTAATTTTGATATTTTAAAAAATACAAATAATGATAAGACTTTAAAATCACTAATTTCAAATAAAGCTAAATTTATTACTGATATTAAACAATTATTAGATTTATTAATTTTATAA", "aa": "MIESYQSYIKNHKHYLKIKELDDAEITILVCYINLNEQKDFYFYGASSNSTLDEKINFDILKNTNNDKTLKSLISNKAKFITDIKQLLDLLIL", "aa_hexdigest": "6b23e4ed02d223bce246895467295ab8", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0005EEBE22", "ncbi_nrp_id": "WP_045470449.1", "uniref100_id": "UniRef100_UPI0005EEBE22", "db_xrefs": [ "SO:0001217", "UniParc:UPI0005EEBE22", "RefSeq:WP_045470449.1", "UniRef:UniRef100_UPI0005EEBE22" ] }, "ips": { "uniref100_id": "UniRef100_UPI0005EEBE22", "uniref90_id": "UniRef90_UPI0005EEBE22" }, "psc": { "uniref90_id": "UniRef90_UPI0005EEBE22", "uniref50_id": "UniRef50_A0AAC9X6Z6" }, "pscc": { "uniref50_id": "UniRef50_A0AAC9X6Z6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9X6Z6" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10923.431599999984, "isoelectric_point": 6.061219215393066 }, "id": "NKIECMJPJD_656", "locus": "GCA000828735_00641" }, { "type": "cds", "sequence": "contig_1", "start": 384642, "stop": 384743, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAAACAAAATACTATTAGCAGAACACGAAAATTCAACGGTTGTTGCTAAATATATGATTAACCTTAGTAAAAATTCTAAAGTGATTCAATCCTTATGA", "aa": "MENKILLAEHENSTVVAKYMINLSKNSKVIQSL", "aa_hexdigest": "7c856d782837eba88f7871670cf02785", "start_type": "ATG", "rbs_motif": "AGGAG(G)/GGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3746.355700000001, "isoelectric_point": 8.162035942077637 }, "id": "NKIECMJPJD_657", "locus": "GCA000828735_00642" }, { "type": "cds", "sequence": "contig_1", "start": 384764, "stop": 385141, "strand": "-", "frame": 2, "gene": null, "product": "restriction endonuclease subunit S", "db_xrefs": [ "EC:3.1.21.3", "KEGG:K01154", "RefSeq:WP_171815249.1", "SO:0001217", "UniParc:UPI00016C0799", "UniRef:UniRef100_UPI00016C0799", "UniRef:UniRef50_UPI002FE3A13A", "UniRef:UniRef90_UPI002FE3A13A" ], "nt": "GTGCCTGGAAATGGAGATTTAGGAAATGTTAACTATTTTAAAGGTAAATTTGATGCTTATCAAAGAACATATGTTTTGATGGAACCAAATGAAAATATAAACATCAAATTTGTATATCATTTGCTTAAAAATTCTCTAAAAAATTATTTTTCAACAAACCAAAAAAATTCTGTTATTTCTTATATAAAGCTCCCAATTTTAGAAAATTTTACTATTCCTATCCCTCACATTTCAATCCAAAATAAAATTGTAGAAATTCTAGATAAATTAGAAGCATATACAAAAGATATTGGCGTAGGATTGCCTTTTGAAATCAAACAACGCAAAAAACAATATGAATATTATCGCAATAAATTATTAGATTTTAAAAAATATTAA", "aa": "MPGNGDLGNVNYFKGKFDAYQRTYVLMEPNENINIKFVYHLLKNSLKNYFSTNQKNSVISYIKLPILENFTIPIPHISIQNKIVEILDKLEAYTKDIGVGLPFEIKQRKKQYEYYRNKLLDFKKY", "aa_hexdigest": "9c302ded6cd56524795d2c0eb1a5a148", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00016C0799", "ncbi_nrp_id": "WP_171815249.1", "uniref100_id": "UniRef100_UPI00016C0799", "db_xrefs": [ "SO:0001217", "UniParc:UPI00016C0799", "RefSeq:WP_171815249.1", "UniRef:UniRef100_UPI00016C0799" ] }, "ips": { "uniref100_id": "UniRef100_UPI00016C0799", "uniref90_id": "UniRef90_UPI002FE3A13A" }, "psc": { "uniref90_id": "UniRef90_UPI002FE3A13A", "product": "restriction endonuclease subunit S", "ec_ids": [ "3.1.21.3" ], "uniref50_id": "UniRef50_UPI002FE3A13A", "kegg_orthology_id": "K01154" }, "pscc": { "uniref50_id": "UniRef50_UPI002FE3A13A", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FE3A13A" ], "product": "restriction endonuclease subunit S" }, "genes": [], "id": "NKIECMJPJD_658", "locus": "GCA000828735_00643" }, { "type": "cds", "sequence": "contig_1", "start": 385793, "stop": 386536, "strand": "-", "frame": 2, "gene": null, "product": "restriction endonuclease subunit S", "db_xrefs": [ "EC:3.1.21.3", "KEGG:K01154", "SO:0001217", "UniRef:UniRef50_UPI00045651C7", "UniRef:UniRef90_UPI00045651C7" ], "nt": "ATGAATAAAATTGAAAAATTAATTAATGAGTTCTGCCCTAATGGAGTGGAGTTTAAAAAATTAAAAAATATAATTACAGTGGCTCCTAAATCACCTTTTGGAGTTACAAAACTTTTAAAAATGGAAAAGGGAAATTATTTAACAATAACATCAGGGAAGAAATCATTTTATGTTGACAATTTTTTAGTTGATGGAGAATATATATTTGTTAATGATGGCGGTCAAGCAGATATAAAATATAATTTTGGAAAAACAATGTATTCTGATCATATATTCGCATTCAAAGTAAATGAATATAACACAAAATATCTTTACTTCTATCTATTAAATATTTCTAATTTTATTAATAAAAAATTGTTTATAGGTAGTACATTAAAAAATTTAAATAAAAAAGAATTTTTAAATCTTGCAATCCCTATTCCGCCCATTTCAATCCAAAATAAAATTGTTGAGATTTTAGATAAATATACAGAATTAGAAACAGAATTAGAAACAGAATTAGAACTTAGAAATAAGCAGTATATATATTATCGCAATGAATTATTAGATTTTAATAAAAATCAAGCATTATTAAAGAAAATAATGTGCAGTGATGATATTGAATCTATTGATAGTAAAATTGAGTTTAAAAAAATGTCAGAAGTAGTATTAAAAAGCTCTTTCAAACAAATTGATGCAGATTCATTATTTTCTTTAAATGAAGGATTTGGCGAAGTTAAATTATTACCAAGTTCTAAAAATTGA", "aa": "MNKIEKLINEFCPNGVEFKKLKNIITVAPKSPFGVTKLLKMEKGNYLTITSGKKSFYVDNFLVDGEYIFVNDGGQADIKYNFGKTMYSDHIFAFKVNEYNTKYLYFYLLNISNFINKKLFIGSTLKNLNKKEFLNLAIPIPPISIQNKIVEILDKYTELETELETELELRNKQYIYYRNELLDFNKNQALLKKIMCSDDIESIDSKIEFKKMSEVVLKSSFKQIDADSLFSLNEGFGEVKLLPSSKN", "aa_hexdigest": "16033043b3baea20d3345dcf9ced7d2d", "start_type": "ATG", "rbs_motif": "AGxAG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 385490, "stop": 386536, "observations": { "mutation": [ 385696, 385789, 385795 ], "stop": [ 385696, 385789, 385795 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00045651C7", "query_cov": 1.0, "subject_cov": 0.6237373737373737, "identity": 1.0, "score": 470.0, "evalue": 4.84e-165, "gene_start": 1, "gene_end": 247, "reference_start": 1, "reference_end": 247, "reference_sequence": "MNKIEKLINEFCPNGVEFKKLKNIITVAPKSPFGVTKLLKMEKGNYLTITSGKKSFYVDNFLVDGEYIFVNDGGQADIKYNFGKTMYSDHIFAFKVNEYNTKYLYFYLLNISNFINKKLFIGSTLKNLNKKEFLNLAIPIPPISIQNKIVEILDKYTELETELETELELRNKQYIYYRNELLDFNKNQALLKKIMCSDDIESIDSKIEFKKMSEVVLKSSFKQIDADSLFSLNEGFGEVKLLPSSKNWDWFCNIKNVNNTYLNKGEVITFGRARYANIKYWNGYFVSSNNITITSKDSSFLLNKFLYYFLVLHNEKFYIETSTYPKFENKIFDDFIIPIPPISIQNKIVEVLDKLETYTKDINTGLPLEIEQRKKQYEYYRNKLLDFDNIARERAK" }, "paralog": false, "identity": 0.9913793103448276, "score": 671.0, "evalue": 8.59e-247, "description": "Internal stop codon at 385696, 385789, 385795. Nonsense mutation around 385696, 385789, 385795.", "psc": { "uniref90_id": "UniRef90_UPI00045651C7", "product": "restriction endonuclease subunit S", "ec_ids": [ "3.1.21.3" ], "uniref50_id": "UniRef50_UPI00045651C7", "kegg_orthology_id": "K01154", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00045651C7", "EC:3.1.21.3", "UniRef:UniRef50_UPI00045651C7", "KEGG:K01154" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00045651C7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00045651C7" ], "product": "restriction endonuclease subunit S" } }, "id": "NKIECMJPJD_659", "locus": "GCA000828735_00644" }, { "type": "cds", "sequence": "contig_1", "start": 386585, "stop": 386746, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATAGATTCAAATGGACAAAAAGCAAAATTAGTCCAAAAAATGATAAATAAAAATAGTAGAGTTAATACTTTTAGTTATTGGCATATTGAAAGAAAAAATACTTTAGTTTCAATAGAAGATGTAAGAGTTTCTTATAGAAAATATATAATGCATAGATAA", "aa": "MIDSNGQKAKLVQKMINKNSRVNTFSYWHIERKNTLVSIEDVRVSYRKYIMHR", "aa_hexdigest": "220a369ca4f8fd8a450639e9bb2f9ca7", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6427.4034, "isoelectric_point": 10.28098278045654 }, "id": "NKIECMJPJD_660", "locus": "GCA000828735_00645" }, { "type": "cds", "sequence": "contig_1", "start": 387254, "stop": 388045, "strand": "+", "frame": 2, "gene": null, "product": "Lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9X6L2", "UniRef:UniRef90_UPI0001794148" ], "nt": "ATGAAATATAAAAAATATCAATCAATTTGGTTAAAAATAAGTGCTGTTATTGCGATTCCCTTATTAATAACAACGATTACAGCATGTGCGAGGATTGATAAGAAACAAAAAAGCAAAGAAGAATTACAATCAAGTTTAAATCAAGCTGTTTTATTAAAAGAAATTGTTGATCAATCATCATATGAAGAATTTAAAAAAAGATATAACCAAACACTTAAATTAAATTTAGCAAATATTGATCAATTATCAGAATCAAAATTAAACCAACAAAAAACAATAATTAACAACTTAATATCACAAGTTGATATATTATTTGAATTAGATGCTATCAAAAGGATTAATAATTTGATAGATCTTAATGGATTAGAAAATATTAAAAAACAATTAGAAGATTTTTTGAACCAATATGAAAAACTACTTTACCAAACAAATGTTGATATGACAATAATAAATAGTTTAAAAACAGAAGCTAATCAAAAAAAATTAGATACTAAAGCAATAATGCAAAAAACTATTTTAGATTTAGTTCATCAAATCAAAAATTTAATAAAGAAATCAGGATCTACTAAAAAGATTGATGAACTCGAAGCTGATATTCAAAATTTAATTAGTGATACTCCAAAAGATGATCATTTAAAATTTTATAAAGATTTTAAAATCATTTATAAGAAGTTAAATGATTTTAATTATTTGATCAACAAAGAAATCACGGCCCAACCTTTTATTAAAAAATTAGACGATATAGTTAATGTTATTAATACTATTTATGAACAAAATCATCATTTTCTATGA", "aa": "MKYKKYQSIWLKISAVIAIPLLITTITACARIDKKQKSKEELQSSLNQAVLLKEIVDQSSYEEFKKRYNQTLKLNLANIDQLSESKLNQQKTIINNLISQVDILFELDAIKRINNLIDLNGLENIKKQLEDFLNQYEKLLYQTNVDMTIINSLKTEANQKKLDTKAIMQKTILDLVHQIKNLIKKSGSTKKIDELEADIQNLISDTPKDDHLKFYKDFKIIYKKLNDFNYLINKEITAQPFIKKLDDIVNVINTIYEQNHHFL", "aa_hexdigest": "e44665b1911025a2bd0ef702e8cbc6c4", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 387254, "stop": 388348, "observations": { "mutation": [ 388043 ], "stop": [ 388043 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0001794148", "query_cov": 1.0, "subject_cov": 0.42694805194805197, "identity": 0.973, "score": 484.0, "evalue": 5.09e-167, "gene_start": 1, "gene_end": 263, "reference_start": 1, "reference_end": 263, "reference_sequence": "MKYKKYQSIWLKISAVIVIPLLITMITACARIDKKQKSKEELQSSLNQAVLLKEIVDQSSYEEFKKRYNQTLKLNLANIDQLSESKLKEQKTIINNLISQVDILFELDAIKRINNLIDLNGLENVKKQLEDFLNQYEKLLYQTNVDMTIINSLKTEANQKKLDTKAIMQKTILDLVHQIKNLIKKSGSTKKIDELEADIQNLISDTPKDDHLKFYKDFKIIYKKLNDFNYWINKEIKAQPFIKKLDDIVNVINTIYEQNHHFLWFDQTKLTNLRQILIKNINHVLNVKKTLEIKEIKTLTNQLLLDLQNYLEGLKMIALESENSINKLISIIESLQPDDYNPYYSIEKQENKQKDENNSQATYNGLEHQIKSLKSIINLFNFNITNLSIDAIYNLNDELIKKFQKANSQINEVVIRIKQTILSNFKQTYLFKDFIKHLKEFNIALNNENDPIKKANRIQTIQEVLKSYEKDLFLNTKINLKLAPNEVLTVNNLEKIDLTYSSDLLQKPQELIAYLELNNNQEREFSKELENLSVRVFTIIDKKLYIPKAKELINESKKIIQTKNEHTNNKTKLQRLEKFTSFVEQGISNENVSSYTLNLYIQRLTKLIQEIKKPQH" }, "paralog": false, "identity": 0.9642857142857143, "score": 680.0, "evalue": 2.09e-246, "description": "Internal stop codon at 388043. Nonsense mutation around 388043.", "psc": { "uniref90_id": "UniRef90_UPI0001794148", "uniref50_id": "UniRef50_A0AAC9X6L2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0001794148", "UniRef:UniRef50_A0AAC9X6L2" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAC9X6L2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9X6L2" ], "product": "Lipoprotein" } }, "id": "NKIECMJPJD_661", "locus": "GCA000828735_00646" }, { "type": "cds", "sequence": "contig_1", "start": 388103, "stop": 389104, "strand": "+", "frame": 2, "gene": null, "product": "Unique hypothetical", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9X6L2", "UniRef:UniRef90_Q9PQ15" ], "nt": "GTGTTAAATGTTAAAAAAACATTAGAGATCAAAGAAATCAAAGCTTTAACTAATCAACTTTTATTTGACCTTCAAAATTACTTAGAAGGTTTAAAAGCGATTGCGTTAGAAAGTGAAAATAGTATTAATAAATTAATTAGTATTATTGAATCATTACAACCAGATGATTATAATCCCTACTATAGTATTGAAAAACAAGAAAATAAGCAAAAAAATGAAAACAATTCTCAAGCAACATATAATGGTTTAGAACACCAAATTAAAAGTTTAAAATCAATCATTAACTTATTTGATTTTAACATTACTAATTTATCAATTGATGCAATCTATAATCTTAATGATGAATTAATTAAAAAATTTCAAAAAGCTAATTCACAAATTAATGATGTTGTGATTAAAATTAAACAAACCATTTTATCAAATTTTAAACAAATTTATTTATTTAAGGACTTCATTAAACATTTAAAAGAGTTTAATATTGAATTAAATAATGAAAACGATCCAATAAAAAAAGCTAATCGAATTCAAACCATTCAAGAAGTCTTAAAAAGTTATGAAAAGGATTTATTTTTAAATACTAAAATTAATTTAAAACTTGCTCCAAACGAAGTATTAACAGTAAATAATCTTGAAAAAATTGATTTAACATATGCTTCTGATTCGCTGCAAAAACCACAAGAATTAATCGCTTATTTAGAATTAAATAATAATCAAGAAAGAGAATTTAGCAAAGAACTTGAAAATCTTAGTGTTAGAGTTTTTACTATCATTGATAAAAAACTTTATATTCCCAAAGCTAAAGAATTGATTGATGAAAGCAAAAAAATTATTCAAACAAAAGATGAGCACGCAAATAATAAAACAAAATTACAACGATTAGAAAAATTCACTAGTTTTGTTGAACAAGGAATAAGTAATGAAAATGTTTCTTCTTATACTCTTAATCTTTATATTCAAAGATTAACAAAACTAATTCAAGAAATTAAAAAACCACAGCATTAA", "aa": "MLNVKKTLEIKEIKALTNQLLFDLQNYLEGLKAIALESENSINKLISIIESLQPDDYNPYYSIEKQENKQKNENNSQATYNGLEHQIKSLKSIINLFDFNITNLSIDAIYNLNDELIKKFQKANSQINDVVIKIKQTILSNFKQIYLFKDFIKHLKEFNIELNNENDPIKKANRIQTIQEVLKSYEKDLFLNTKINLKLAPNEVLTVNNLEKIDLTYASDSLQKPQELIAYLELNNNQEREFSKELENLSVRVFTIIDKKLYIPKAKELIDESKKIIQTKDEHANNKTKLQRLEKFTSFVEQGISNENVSSYTLNLYIQRLTKLIQEIKKPQH", "aa_hexdigest": "6aa7a244eaf161245ced5ac922d940c4", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 387803, "stop": 389104, "observations": { "mutation": [ 388043 ], "stop": [ 388043 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQ15", "query_cov": 0.996996996996997, "subject_cov": 0.538961038961039, "identity": 0.961, "score": 599.0, "evalue": 5.35e-211, "gene_start": 2, "gene_end": 333, "reference_start": 285, "reference_end": 616, "reference_sequence": "MKHKKYKSIWLKLSMISAIPLLATIITACAKVDTKQKAKEELQSSLNKAVLLKEIVDQSSYEDFKKIYNQTLKSNLENINKLSEQKLKQEQQTIKNLITQVSVLNELDSIKNMSDLIDVVELENTKKQLQDFLNQYEKLLNQASVDTTIIDKLKSDAHQKRLDTKALIQKTILDLANKIKSLIKTSGLNKNIVELENDIQKLVNNDTKANFKKLYEDFKLIYTKLNDINDLINKELKALPFIKKLDDIVNVINTIYEQNHHFLWFDQTKLTNLRQILIKNINHVLNAKKTLEIKEIKTLTNQLLFDLQNYLEGLKMIALESENSVNKLISIIESLQPDDYNPYYSIEKQENKQKDENNSQATYNGLEHQIKSLKSIINLFDFNIANLSIDAIYNLNDELIKKFQKANSQINEVVIQIKQTILSNFKQTYLFKDFIKHLKEFNIELNNENDPIKKANRIQTIQEVLKSYEKDLFLNTKINLKLAPNEVLTVNNLEKIDLTYASDLLQKPQELIAYLELNNNQEREFSKELENLSVRVFTIIDKKLYIPKAKELINESKKIIQTKDEYTNNKTKLQRLEKFTSFVEQGISNENVSSYTLNLYIQRLTKLIQEIKKPQH" }, "paralog": false, "identity": 0.9076212471131639, "score": 761.0, "evalue": 1.94e-277, "description": "Internal stop codon at 388043. Nonsense mutation around 388043.", "psc": { "uniref90_id": "UniRef90_Q9PQ15", "product": "Unique hypothetical", "uniref50_id": "UniRef50_A0AAC9X6L2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQ15", "UniRef:UniRef50_A0AAC9X6L2" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAC9X6L2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9X6L2" ], "product": "Lipoprotein" } }, "id": "NKIECMJPJD_662", "locus": "GCA000828735_00647" }, { "type": "cds", "sequence": "contig_1", "start": 389214, "stop": 389573, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATATAAAAGAAAGAAATAATAATAATGTAGATTTTGAACTTCATTTAGGTTCAAGAGACTATGCATTTGAAAATAAGCAAAAATATCGTGTTACACTAGCGTCAGTAAACACACCCCAAACCGAAATTTCTAAGGAATTTTTGCTTTCTGATGTTAAATTAGATTATCAAGATGGTAATATTGGCATATTAAAATGCCATTTTGATAACTTAACACCAAACACTGCATACACATTAAAAGAAATTAAATTTCTTGCTAACTCAAATGAACAACAAAGCACAAAACCACATAAGGTAATTAAACCTTTTAATGATGATAACAATGTTATTATCGATAAAAATTATCCTCAAAAATGA", "aa": "MDIKERNNNNVDFELHLGSRDYAFENKQKYRVTLASVNTPQTEISKEFLLSDVKLDYQDGNIGILKCHFDNLTPNTAYTLKEIKFLANSNEQQSTKPHKVIKPFNDDNNVIIDKNYPQK", "aa_hexdigest": "9631798eac7607e99ff4c932d3245d96", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13835.319499999976, "isoelectric_point": 6.279367256164551 }, "id": "NKIECMJPJD_663", "locus": "GCA000828735_00648" }, { "type": "cds", "sequence": "contig_1", "start": 389718, "stop": 389954, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAATGCACCAACAGTTTTACAATCAATAAAATTTGAAAATTGTGATGCTAATGGTGTAATTAATAATGAAATGGAAGGAAATGTAAATTTTTCAATAACAACAAATAAGGTTAATGATGATTTTGCTAACCGAAAAGTTAAATTCATATATCGTGACAATAACAATGTTGCTTATGAAACTGATGGTATTAAATTAATTGCAACAGACACAAGTTATCATTTTTCTTTATGA", "aa": "MKNAPTVLQSIKFENCDANGVINNEMEGNVNFSITTNKVNDDFANRKVKFIYRDNNNVAYETDGIKLIATDTSYHFSL", "aa_hexdigest": "cf27fbf4f701bec2ccb8353dd28ac1a1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8837.722299999998, "isoelectric_point": 5.145486259460449 }, "id": "NKIECMJPJD_664", "locus": "GCA000828735_00649" }, { "type": "cds", "sequence": "contig_1", "start": 390309, "stop": 390551, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTTTATGAATATAATTATCGGGGTGATATCAAAGCAGTTGAATCTGATTTAATTACTCTTAAAAAAGACCGAAATGTTTATCAAATAAATTTACCAATCAGCGTTCCTAACCGAAAATATACTTTTAAGAAAGTTTTAATTCAAGAACGCAATAATTTAAACGACTATGTGGATTTAAATAAAAAGATAAACTTAACAGATTCATTTGTAGTTAGCCCTGGAAAAACAACATTTGAATGA", "aa": "MVYEYNYRGDIKAVESDLITLKKDRNVYQINLPISVPNRKYTFKKVLIQERNNLNDYVDLNKKINLTDSFVVSPGKTTFE", "aa_hexdigest": "94811ed51d1908017a0bc0a54ea32600", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9432.679199999995, "isoelectric_point": 9.187212181091308 }, "id": "NKIECMJPJD_665", "locus": "GCA000828735_00650" }, { "type": "cds", "sequence": "contig_1", "start": 390642, "stop": 390830, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATTGATTTTAATTTAAATAATTTAAAACCACAAACCACTTACCATTTAAAATCAATAACAATAGCAAAACCAAATAAAGCATATACTAATTTAAAAATTCAATCACTTCCACAAGTGGATATTAAAACGCAATTACCTAATTTAATCTTACATGATTTAAAAGCCACAAGTGCGGTTTTAAAATGA", "aa": "MIDFNLNNLKPQTTYHLKSITIAKPNKAYTNLKIQSLPQVDIKTQLPNLILHDLKATSAVLK", "aa_hexdigest": "40b21dabf71f8f7407b7c96e5cd1fbef", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7014.194400000001, "isoelectric_point": 9.824224281311036 }, "id": "NKIECMJPJD_666", "locus": "GCA000828735_00651" }, { "type": "cds", "sequence": "contig_1", "start": 390996, "stop": 391796, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTGAAAGAACTAGTGCTGTATTAGCAACAAGTATTAGTGAAATAAGTAATCGTTTGGGAACTGCTTTAACGAATGCACAAGTTAAAATCACTTTAAAAGATGTGGATAATGTTTTATTAGCTAATCAAAAAGCGATTATTAACTATGGTAATAATCAAAAGAGTGAAGCAACTGTTATTGTTAACAATGGTGTTAAGTATCTAATAGCAACCTTAACTAAATTAACATTAAATACGCCTACAATAATTAAATCCATTACATTTGAACAAAAACCAACATGTGCTATTGAAAATATTGGGTTAGATAAAACAAATAACATTATTTATCAAAATACACATGAAAAAGCAATTCCTTTGGTAATTAATAATAATTTTGAAATTAATGGGCCATTAGCATCAAGTCATGAAAGTTTAAAAAGTATTAATGCATCTGATGCTAAAAAGGTAAATGCAATTAATTTAACCCTTGACTTTAATACCAATGAACATATTTATAAAAATTTATTTTTTAAACTAAAATATGTCCCTATTGACAATCAAGGTAATGAAAATATTTATGACGCATCAGAAGCAATTTATAGTGATGTTTTAAGTGCAAATAATATAGTTGCTAATAAAATCCAATTTAGTTTAGGTAATTTACAAACTAATCGTAAATATAAATTAAAAACAATTTATTATTTGACCTCAAAAACAGCACCTTTAGATGATCATAACGTTGTAATGATCAAACCAAATGTTAAATCCGAAATTATTGTTGCCCCTAATGATAGTACGATTGTAAAATATGGTAATTGA", "aa": "MVERTSAVLATSISEISNRLGTALTNAQVKITLKDVDNVLLANQKAIINYGNNQKSEATVIVNNGVKYLIATLTKLTLNTPTIIKSITFEQKPTCAIENIGLDKTNNIIYQNTHEKAIPLVINNNFEINGPLASSHESLKSINASDAKKVNAINLTLDFNTNEHIYKNLFFKLKYVPIDNQGNENIYDASEAIYSDVLSANNIVANKIQFSLGNLQTNRKYKLKTIYYLTSKTAPLDDHNVVMIKPNVKSEIIVAPNDSTIVKYGN", "aa_hexdigest": "54e0125e96f5ee67279a5b03b892ff7d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 29444.23990000003, "isoelectric_point": 8.899554252624515 }, "id": "NKIECMJPJD_667", "locus": "GCA000828735_00652" }, { "type": "cds", "sequence": "contig_1", "start": 392007, "stop": 392549, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF07198.17" ], "nt": "ATGGGAGAAGTTAGTTTTGATGTTTCACAATTAAAAAATAATAATTTATATGAGGTAATTGCTTTACAAACACAAGACCAAACTAAAATCGTTAATGTTAATGAAATTGAATACAGCAATAAGATCATAAATAATTTAAATATTGATGAATTAAATACACCATACCAATATAGCAAAAATGGTGATCTTAACTTAATTGCTAAGATACCATTACGCTATGCTAATGAACAAGTCTATGGTGTTTTTGAAGACCAAAATAACCAAAAACATTTAATTCCTGCTGTAAATAAAAATGATGGAACAATAATGTTTGATACTCAAGTTTTAACTAAAAATCCCAATAAAAAATATCATTTACAAAAAGTTGTTTTAGCAAAAAATCAAAATCAAATTTTAATACATAATTTTGATTTAGTAAGTAATCAAAAACAAGATATCTTCTTAAAAGAATATAAAGTTATTAGTATTAGTGCTCTAGAAAATGATGCTGATAAAACTAAAAGAGAAATCAATTTAAAACTATCTAACATAAAAAATGACTGA", "aa": "MGEVSFDVSQLKNNNLYEVIALQTQDQTKIVNVNEIEYSNKIINNLNIDELNTPYQYSKNGDLNLIAKIPLRYANEQVYGVFEDQNNQKHLIPAVNKNDGTIMFDTQVLTKNPNKKYHLQKVVLAKNQNQILIHNFDLVSNQKQDIFLKEYKVISISALENDADKTKREINLKLSNIKND", "aa_hexdigest": "cfc14130079a72e0f47fb489768add7b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07198.17", "name": "DUF1410", "length": 60, "aa_cov": 0.3277777777777778, "hmm_cov": 0.95, "evalue": 6.704033769674639e-17, "score": 57.242347717285156, "start": 73, "stop": 131 } ], "seq_stats": { "molecular_weight": 20795.268700000015, "isoelectric_point": 6.309889793395997 }, "id": "NKIECMJPJD_668", "locus": "GCA000828735_00653" }, { "type": "cds", "sequence": "contig_1", "start": 393176, "stop": 393502, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGACATTAGTTGAAGCTGAAAATCAAAACAATGTTGTTGTTGGTGACTTTTTACTTGCTGGCACTTCTATTAATAAAAATGATAGCCCAATTGCTGATAATAATGATAAGAACAAAGATATAGCAACTTTGAGCTGTAATTTTGAAAACAAACTAAAAAAAGGAACTAAATACCTATTAAAAGAAGTTCGATTTATTGCTAATCCAAATGAAGAAATTAGTGCTAAACCTACTAGAGCTGCTAGACCATTTAATAATGATAATGTTATTTATAGTTTGAATCTACAAATAAATGATCCTTATGACTTTACAACTTTAAAAGACTAG", "aa": "MTLVEAENQNNVVVGDFLLAGTSINKNDSPIADNNDKNKDIATLSCNFENKLKKGTKYLLKEVRFIANPNEEISAKPTRAARPFNNDNVIYSLNLQINDPYDFTTLKD", "aa_hexdigest": "3e625499dfac96c7e80849e447a8241e", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12121.42819999998, "isoelectric_point": 5.110246086120605 }, "id": "NKIECMJPJD_669", "locus": "GCA000828735_00654" }, { "type": "cds", "sequence": "contig_1", "start": 393761, "stop": 394105, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGAGCAAACAGAGTATTACATCTATAAAATTCAGTTTATAGATGAATTAGAATATTCAAAATTACCACCAAATCGTAATAACATTGTTTATGAATATAATAAAAATATTGCACGTAATTATACATTCAGAACTAAAACTGCCCCTTTAATTTTAGAAAAAATAGAAAAACATGATAATGGTGGCGTTGGAGGACAAAATTTTTATTTTTATCTAAATAAAGCTAATGATGATCCTAGTTCAATCAAACAAATTACCATATATTGTCGTGATAATAACAATGAATTATATACTTCTGATAATATAGAAATAGGTTTTGATGATCTTATATATCATTCTTGA", "aa": "MKEQTEYYIYKIQFIDELEYSKLPPNRNNIVYEYNKNIARNYTFRTKTAPLILEKIEKHDNGGVGGQNFYFYLNKANDDPSSIKQITIYCRDNNNELYTSDNIEIGFDDLIYHS", "aa_hexdigest": "f5e2852a8a3195dfcb6ebe21ced96a3c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13581.926999999983, "isoelectric_point": 5.116555213928222 }, "id": "NKIECMJPJD_670", "locus": "GCA000828735_00655" }, { "type": "cds", "sequence": "contig_1", "start": 394328, "stop": 394543, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGAAATTGCATCACAACCTTTCAAATTAGAAAAAAACAAAACAACATATAACAATATTCTTTTACCAATTAGTATTCCAAATCGCTTATATCATTTTAAAAAAGTAGTAATTCAAAAATTAAATCAACCAAATAAATCTATTGATTTAGATCATGATCCAAATTTAAAATCTTTTTTTATTGTTGGTCCTGGTAAAACAGATTTTGTATGA", "aa": "MKEIASQPFKLEKNKTTYNNILLPISIPNRLYHFKKVVIQKLNQPNKSIDLDHDPNLKSFFIVGPGKTDFV", "aa_hexdigest": "456a4587ed23f3180e8ad8c19351636a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8225.5204, "isoelectric_point": 9.698575401306151 }, "id": "NKIECMJPJD_671", "locus": "GCA000828735_00656" }, { "type": "cds", "sequence": "contig_1", "start": 394652, "stop": 394993, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF07198.17" ], "nt": "GTGGTTGCTCAAATTAATGATAACAATGATTATATAATGCATTTTACGAATCCTAATTTAGTTAATAAGAAAGTTAAATTAACATTCAAAGTTGATGATCAAGATGATCATACAAAAACCATTGAAGCTATCATTGGATTAGATGGAAAGGCTATTTTTAAAACAAGTGATAATACAATTTTTGCACCAGATCATAAATATACACTAACAAAAATTGAAGCTAATAACAAAAAAGTTGCTAATATTAATGAAATTCCATTAAAAAATAAAATCGTTGATAAAAATCCAATTAAAAGAATTAACTTAACACAACTAAAAGTTGCTAAAGATTTAATTCTTTGA", "aa": "MVAQINDNNDYIMHFTNPNLVNKKVKLTFKVDDQDDHTKTIEAIIGLDGKAIFKTSDNTIFAPDHKYTLTKIEANNKKVANINEIPLKNKIVDKNPIKRINLTQLKVAKDLIL", "aa_hexdigest": "116d4089399fc5d6ea3b8251380ffdd1", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07198.17", "name": "DUF1410", "length": 65, "aa_cov": 0.5752212389380531, "hmm_cov": 0.9230769230769231, "evalue": 1.8644781351552376e-14, "score": 49.41379165649414, "start": 17, "stop": 81 } ], "seq_stats": { "molecular_weight": 12870.771899999978, "isoelectric_point": 9.273857688903806 }, "id": "NKIECMJPJD_672", "locus": "GCA000828735_00657" }, { "type": "cds", "sequence": "contig_1", "start": 395807, "stop": 396058, "strand": "-", "frame": 2, "gene": "ureE", "product": "UreE urease accessory N-terminal domain-containing protein", "db_xrefs": [ "RefSeq:WP_004026601.1", "SO:0001217", "UniParc:UPI00000C1C80", "UniRef:UniRef100_A0AAX1QXP8", "UniRef:UniRef50_A0AAP9ADA3", "UniRef:UniRef90_A0AAP9ADA3" ], "nt": "ATGATTTATAAAGAAATTGTTCGTAATATTATCAAGCATCCATTAATCTTACCAGAAGGAAAACATTACCATTTACATAAAATTCTAACTTCAAAAGATGAATTACGTAAGGGTGAAGTTAGTTTAGTTGCAGAAAATGGCAAAGAATATACTGTTGATTTATCTGCATTTGTTGACTTGTTAGTTGATGGTGATTGCATTATTGATGATAATCATTCACTTGTTGCTTTATACTTTGATGAAAGTAAATAA", "aa": "MIYKEIVRNIIKHPLILPEGKHYHLHKILTSKDELRKGEVSLVAENGKEYTVDLSAFVDLLVDGDCIIDDNHSLVALYFDESK", "aa_hexdigest": "bb146b024a5a55c70ea90580491ab26b", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI00000C1C80", "ncbi_nrp_id": "WP_004026601.1", "uniref100_id": "UniRef100_A0AAX1QXP8", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1C80", "RefSeq:WP_004026601.1", "UniRef:UniRef100_A0AAX1QXP8" ] }, "ips": { "uniref100_id": "UniRef100_A0AAX1QXP8", "uniref90_id": "UniRef90_A0AAP9ADA3" }, "psc": { "uniref90_id": "UniRef90_A0AAP9ADA3", "product": "UreE urease accessory N-terminal domain-containing protein", "uniref50_id": "UniRef50_A0AAP9ADA3" }, "pscc": { "uniref50_id": "UniRef50_A0AAP9ADA3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAP9ADA3" ], "product": "UreE urease accessory N-terminal domain-containing protein" }, "genes": [ "ureE" ], "id": "NKIECMJPJD_673", "locus": "GCA000828735_00658" }, { "type": "cds", "sequence": "contig_1", "start": 396216, "stop": 396356, "strand": "-", "frame": 1, "gene": null, "product": "MTA/SAH nucleosidase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B5ZBW7", "UniRef:UniRef90_B5ZBW7" ], "nt": "ATGGAATTAGCAGCTATTGCTCATGTATGCACGCGTATGTTAACACCACTAGTAAGTATTAAATTAGTTAGTGATCATATTACTCTTCCCAATTCCAATCAAGAACAGTTTAATAAAAACTTATCACTAATTGATAAATGA", "aa": "MELAAIAHVCTRMLTPLVSIKLVSDHITLPNSNQEQFNKNLSLIDK", "aa_hexdigest": "629b629f4f97bfaa7deb8854afa07138", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 396198, "stop": 396371, "observations": { "mutation": [ 396218 ], "stop": [ 396218 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_B5ZBW7", "query_cov": 1.0, "subject_cov": 0.7666666666666667, "identity": 0.87, "score": 79.3, "evalue": 1.05e-18, "gene_start": 1, "gene_end": 46, "reference_start": 6, "reference_end": 51, "reference_sequence": "MDLVDMELAAIAHVCTRMLTPLVSIKMVSEHIMHPSSNQEQFNKNLVLIDKWFNKYLLEC" }, "paralog": false, "identity": 0.8245614035087719, "score": 95.5, "evalue": 5.84e-28, "description": "Internal stop codon at 396218. Nonsense mutation around 396218.", "psc": { "uniref90_id": "UniRef90_B5ZBW7", "product": "MTA/SAH nucleosidase", "uniref50_id": "UniRef50_B5ZBW7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_B5ZBW7", "UniRef:UniRef50_B5ZBW7" ] }, "pscc": { "uniref50_id": "UniRef50_B5ZBW7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B5ZBW7" ], "product": "MTA/SAH nucleosidase" } }, "id": "NKIECMJPJD_674", "locus": "GCA000828735_00659" }, { "type": "cds", "sequence": "contig_1", "start": 396441, "stop": 396857, "strand": "-", "frame": 1, "gene": null, "product": "Purine nucleoside phosphorylase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQ20", "UniRef:UniRef90_Q9PQ20" ], "nt": "ATGATCGGTTTAATTGTGGCATTAAATAGTGAAATTAAAACCTTTTTTAAACAAATTAAAAAGAAGGTTTATCAAATTAATAATATTGATTTTTACTTGTGCACTCATCAAAATATAGAATTTGTTTTAGTTTTTACGGATGTAGGAAAAACTAATGCGAGTTTTATTACTGCACTATTAATTAATAATTTTAAACCTAAAGTGATTTTAAATGTTGGTAGTTGTGGTGCACTAAATGATCAGTTACAGGTTTTAGATATTGCCATTATTGATCAATGCCAATACTTAGATGTTAACGTTAGCGCTTTTGGTTATTTAAAAAATCAAATACCGCGTTTAGATAAATTTTTTATTTTAGATAAAAATTATAATCAACAAATCAAAAACCAGTTAATTAAGAAAAAACTAAAATGTTGA", "aa": "MIGLIVALNSEIKTFFKQIKKKVYQINNIDFYLCTHQNIEFVLVFTDVGKTNASFITALLINNFKPKVILNVGSCGALNDQLQVLDIAIIDQCQYLDVNVSAFGYLKNQIPRLDKFFILDKNYNQQIKNQLIKKKLKC", "aa_hexdigest": "08823b62ca1a8a7d83a109ff94af2db0", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_Q9PQ20", "query_cov": 0.8405797101449275, "subject_cov": 0.9830508474576272, "identity": 0.991, "score": 231.0, "evalue": 2.08e-76, "valid": true, "product": "Purine nucleoside phosphorylase", "uniref50_id": "UniRef50_Q9PQ20" }, "pscc": { "uniref50_id": "UniRef50_Q9PQ20", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQ20" ], "product": "Purine nucleoside phosphorylase" }, "genes": [], "id": "NKIECMJPJD_675", "locus": "GCA000828735_00660" }, { "type": "cds", "sequence": "contig_1", "start": 397158, "stop": 397322, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTGTGTTGATTTAATGAATGCCTATGATAAAAAACTAATTGATCAAGCTTATTGTGCGGGAATTTATCATGACATACTAAAATGTTTAGATGAACAAGAAAGTGTTGCATATTTTAATGAACATAAAAGTGAATTAAATATTGGTGATGATTTTATCTCTTGA", "aa": "MCVDLMNAYDKKLIDQAYCAGIYHDILKCLDEQESVAYFNEHKSELNIGDDFIS", "aa_hexdigest": "dc8c8093ff8856a68e99ed1c18fa25b9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6233.962299999999, "isoelectric_point": 4.295174980163575 }, "id": "NKIECMJPJD_676", "locus": "GCA000828735_00661" }, { "type": "cds", "sequence": "contig_1", "start": 397668, "stop": 398045, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01467.32" ], "nt": "ATGAAAATCATTTTGTTTTGTGGAGCTTTTGATATGGTTCACAATGCTCATATTGCAATGGCTAAACAAGCCATGAAGTTAGTTAATGCTGACAAACTAATTTTTTTACCATCAAATTTTAAATTTTTTAAAGCAATTAATAAAAATGACAATTTAGAGTATGAAAAAACAAAATTAACACCTGGACATCATCGTATAGCCATGTTAAAAATTGCAACCAAAAATTTAGTAAATATTGAAGTAAGTGACTACGAATTAAAACAAATTAATAAATCTTATACAATCAATACTATTGAGCATTTTAAACAAATATATGGTAGTGAACATGAATATTATTTTATTATGGGTAGTGATAACTTAGAACGTTTTAAACAATGA", "aa": "MKIILFCGAFDMVHNAHIAMAKQAMKLVNADKLIFLPSNFKFFKAINKNDNLEYEKTKLTPGHHRIAMLKIATKNLVNIEVSDYELKQINKSYTINTIEHFKQIYGSEHEYYFIMGSDNLERFKQ", "aa_hexdigest": "f0a820a00ea8809dfd90026957deaffd", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01467.32", "name": "CTP_transf_like", "length": 119, "aa_cov": 0.952, "hmm_cov": 0.7899159663865546, "evalue": 1.690917383125233e-16, "score": 56.22269821166992, "start": 5, "stop": 123 } ], "seq_stats": { "molecular_weight": 14647.915199999985, "isoelectric_point": 9.11906909942627 }, "id": "NKIECMJPJD_677", "locus": "GCA000828735_00662" }, { "type": "cds", "sequence": "contig_1", "start": 398072, "stop": 399157, "strand": "-", "frame": 2, "gene": "yqeH", "product": "ribosome biogenesis GTPase YqeH", "db_xrefs": [ "COG:COG1161", "COG:J", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0042254", "RefSeq:WP_045470465.1", "SO:0001217", "UniParc:UPI0004563C3B", "UniRef:UniRef100_UPI0004563C3B", "UniRef:UniRef50_B5ZBX1", "UniRef:UniRef90_A0AAC9T0H0" ], "nt": "ATGATAACAAATAAAAAATGTAAAGGCTGTGGAGCTTTTTTGAATGATCAAATCAATACAATTGGATATACCCCAAAAATAAATGAAACAAAAACCAATTTATGCCAACGTTGTTTTAAATTAATACACTATAATAAACTTGAAAAAGTAAGTACTAATTTAAATCAAAGTATTAAAGAGACAATTAACACTTTAAATTTTAGTAATTTACAGATTTTTGCTGTATTAGATATTTTAGATTTAGAACACACAATTATTGATGAACTAAAAGAATATCAAAATAAAATTATTTTTTTAGTTAATAAAATTGATTTATTACCTCAGCGCTACAATAGTGAATTAGTTAATGAAAATGTTGTTAAAACACTTATTAATCATGGATTTATTGATCCTAAAATTATCTATGTTTCCACACATTCTAATACCAGTTTAAAAAAAGTTATGGATTATATTAAAAATGCAACTAATAAAAAACAAAAATCCATTTTTTTTGGAAAATCAAATGTTGGTAAATCAAGTTTAATCAATGCTTTATTAGCGCTAAATAAAATTAAAACTAAACTTACAATTAGTAGTTATACTAATACAACAATTAATTTAAATAAAATTAATATACAAGAACATCAAATACTTGATGCTCCCGGAGTTTGTTTTGATGAAAACATTTTAAATTATGTACGTGATGAAGATAATAAATCAATCATGATTTCTTATGGTGCTAAAGCAATTAATTACCAAATTAATCCCCAACAAGCTCTTATGATTTCTGGATTGGTTGGAATTCAGTATTTAAAGGGTCAAAAAACAACTTTTACATTATATGTTTCATTCCAATTAGATATTCATCGTTGTAAATTAGAAAATTTTATAATGAATTTTAATAAACGATATTTATTAGCGAAGTTCAATTATCTAAATCAAGATCTCGAATTTGTTGACCATCTAATAACTTTAAATAAGGATCAAAAAACCAATATTTGTATTGCAGGCCTAGGTTTGTTAGTTATTAATGCTCATGCTGAACAAATATGTATCCGTTTACCAAAAAGTGTTGGTCTAAAAGTAGCAAAATATGCGATTATTTAA", "aa": "MITNKKCKGCGAFLNDQINTIGYTPKINETKTNLCQRCFKLIHYNKLEKVSTNLNQSIKETINTLNFSNLQIFAVLDILDLEHTIIDELKEYQNKIIFLVNKIDLLPQRYNSELVNENVVKTLINHGFIDPKIIYVSTHSNTSLKKVMDYIKNATNKKQKSIFFGKSNVGKSSLINALLALNKIKTKLTISSYTNTTINLNKINIQEHQILDAPGVCFDENILNYVRDEDNKSIMISYGAKAINYQINPQQALMISGLVGIQYLKGQKTTFTLYVSFQLDIHRCKLENFIMNFNKRYLLAKFNYLNQDLEFVDHLITLNKDQKTNICIAGLGLLVINAHAEQICIRLPKSVGLKVAKYAII", "aa_hexdigest": "6fa6dd3c7b1136afda7f07d3f83d56ae", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "ups": { "uniparc_id": "UPI0004563C3B", "ncbi_nrp_id": "WP_045470465.1", "uniref100_id": "UniRef100_UPI0004563C3B", "db_xrefs": [ "SO:0001217", "UniParc:UPI0004563C3B", "RefSeq:WP_045470465.1", "UniRef:UniRef100_UPI0004563C3B" ] }, "ips": { "uniref100_id": "UniRef100_UPI0004563C3B", "uniref90_id": "UniRef90_A0AAC9T0H0" }, "psc": { "uniref90_id": "UniRef90_A0AAC9T0H0", "gene": "yqeH", "product": "ribosome biogenesis GTPase YqeH", "uniref50_id": "UniRef50_B5ZBX1", "cog_id": "COG1161", "cog_category": "J", "go_ids": [ "GO:0003924", "GO:0005525", "GO:0005737", "GO:0042254" ] }, "pscc": { "uniref50_id": "UniRef50_B5ZBX1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B5ZBX1" ], "product": "Ribosome biogenesis GTPase YqeH" }, "genes": [ "yqeH" ], "id": "NKIECMJPJD_678", "locus": "GCA000828735_00663" }, { "type": "cds", "sequence": "contig_1", "start": 399157, "stop": 399876, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03989.18" ], "nt": "TTGGCTTCTAGATTAAATCTAATTAAAAGAATTGAATTAAGCGAGTTGGATATAAATAAAAATAGTAAACAAATTAGCATTATGAAATTAAATGATAATGATAATTTAATCAGTGCTAATTTAATCAAAAAAGGTCATAATCAATTTATTATTGCAATTTCTAAATTAGGATTAGCCCTGCTATTTTTAGTTCATGAAATTAATTGCTTAAATCGTTTAGCTAAAGGAATTAAAATTATGAAATTAAAACCAAATGATGAGGTTAGTGCTATTTTAATTACTCCTAATAATGGTTATAATGTTCAACTTTTTTTAGAACGGGGAAGTAAGTGTTTTAATATTAGTGAATTAAAATTATCAAAACGTGCAACTACACCAACAAATTTATATCCAATAACAAAAAAAGTGCAAAATGTTTTAGCAGCTTTTTTAGTTGCTCATGAAAATGTTTTTTATCTTTTAGATCAACAACAAAAAATAAATCCATATTACTTATCAAACCCCAAACCAACAAAACTAGATACTAAAATTAGTATCTATGAAAACGATCAAATGATCACTGATGTAGTTAAAGATTGCTTTTTAAGTGATGAAGCAATTAGTGATTTTAAAAAAATTAATATGTACGCAAATGAATTTGATAATGAAATGCTAAAAATTAATAAAAATCACGATGATTCACAATTAGAATTAATTGATGAAGAGGAAGAAAAGAATTAA", "aa": "MASRLNLIKRIELSELDINKNSKQISIMKLNDNDNLISANLIKKGHNQFIIAISKLGLALLFLVHEINCLNRLAKGIKIMKLKPNDEVSAILITPNNGYNVQLFLERGSKCFNISELKLSKRATTPTNLYPITKKVQNVLAAFLVAHENVFYLLDQQQKINPYYLSNPKPTKLDTKISIYENDQMITDVVKDCFLSDEAISDFKKINMYANEFDNEMLKINKNHDDSQLELIDEEEEKN", "aa_hexdigest": "0a7b74dfc34bc77eb9b0b893ebdaf8ae", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03989.18", "name": "DNA_gyraseA_C", "length": 46, "aa_cov": 0.19246861924686193, "hmm_cov": 1.0, "evalue": 1.706957922379526e-18, "score": 61.40036392211914, "start": 49, "stop": 94 } ], "seq_stats": { "molecular_weight": 27431.42310000002, "isoelectric_point": 6.908574867248535 }, "id": "NKIECMJPJD_679", "locus": "GCA000828735_00664" }, { "type": "cds", "sequence": "contig_1", "start": 399988, "stop": 400299, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTGAATTTAAAAAAACTTTTGGTTTCGAACGCAAAACAAAATTATTTGATGAATTAGTAAAAGTTGAAATAAACGAAGATCAAATGATCGAAAAACAAAATTTGAATTTAGTAATAAGTCGTGATGGATACATTAAAACTGTTTCTAAAAAGTCATTTGAATCATCTAAATATGATGAATTAGGATTAAAAACTAATGATATTCTTTTTTATCATAATATCATTAATTCTCATGATAAAATCTTAATCATTACATCAAAAGCTAAATTAATTAATTTAGTTGCTCATAAAATCACTTCTATGCGGTGA", "aa": "MIEFKKTFGFERKTKLFDELVKVEINEDQMIEKQNLNLVISRDGYIKTVSKKSFESSKYDELGLKTNDILFYHNIINSHDKILIITSKAKLINLVAHKITSMR", "aa_hexdigest": "87b32cc6154cec7ffd92bec03b9fdad4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12079.969499999983, "isoelectric_point": 9.30112781524658 }, "id": "NKIECMJPJD_680", "locus": "GCA000828735_00665" }, { "type": "cds", "sequence": "contig_1", "start": 400381, "stop": 401397, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00521.26" ], "nt": "ATGCATGGTAATAAAGGTTCAATTGATGGTGATAATGCTGCTGCAATGCGTTATACAGAAGCCCGATTATCAAAAATTGCTAGTGTAATGTTAGCTAACTTAAAAAAAGATGTTGTTAAATTTAGTCCAAATTTTGATGATAGTGAAAAAGAGCCATCAATATTACCATCTCTTTTTCCTAATTTATTAATCAATGGAGCGACAGGAATTGCTTCTGGTTATGCAACAAATATTCCTCCTCATAACCCTAATGAAGTCTTTGATGCTTTAATTTATCGTATTGATCATCCAGATTGTAGTGTAGAAAAACTAATTAAAATCTGTCCTGCGCCAGATTTTCCTACAGGTGGGGAAATCCACGATTTAAATGGATGTGCTAATGCTCACAAAACTGGTGAAGGTAAATTTATAATTCGTGCTTGCATTGATTTTAAAATCAATGATAATAAGATTAATCAAATTATTATTAGTTCAATCCCTTATGAAACAAACAAGGCTTTAATCATTAAAGAAATTGAAGATATTATTTATAATAAAGAAGTTGCTGGATTAATCGAAGTTCGTGATGAATCTGATGCTAAGGGAATTAGTATAATTATTGATACTAAAAAAGATATTAATTTAGAAAATGTTAAAAACTATTTATATAAAAAAACCAACTTGGAAATCAGCTATAATACAAAATTTATTGCAATTGTTCACCGAACTCCTACACTTGTAAATCTATCTACATATTTAGATGCTCAAATTAATCATAGTTTAGATGTAATTAATAAAGTCGATTTATATGATCTAAACAAAATTTTGTTACGTATTGAAATTGTTGAAGGCTTAATTAAATGCGTTGATTTAATTGATGAAATTATTAAAATTATTCGTGCTAGTGAATCGCGCCAAGACGCAAAAAATACTTTAATTCAAGTTTTTAATTTTACAAATAATCAAGCTGAAGCGATTATTATGATGCGTTTGCATAATTTGACACGTACTGATATATTTGATTTAAAAAATGAATGA", "aa": "MHGNKGSIDGDNAAAMRYTEARLSKIASVMLANLKKDVVKFSPNFDDSEKEPSILPSLFPNLLINGATGIASGYATNIPPHNPNEVFDALIYRIDHPDCSVEKLIKICPAPDFPTGGEIHDLNGCANAHKTGEGKFIIRACIDFKINDNKINQIIISSIPYETNKALIIKEIEDIIYNKEVAGLIEVRDESDAKGISIIIDTKKDINLENVKNYLYKKTNLEISYNTKFIAIVHRTPTLVNLSTYLDAQINHSLDVINKVDLYDLNKILLRIEIVEGLIKCVDLIDEIIKIIRASESRQDAKNTLIQVFNFTNNQAEAIIMMRLHNLTRTDIFDLKNE", "aa_hexdigest": "227192fe0229e5d95e7a1128854a3e9f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00521.26", "name": "DNA_topoisoIV", "length": 339, "aa_cov": 1.0, "hmm_cov": 0.9734513274336283, "evalue": 3.4894279617075204e-96, "score": 318.2030029296875, "start": 1, "stop": 338 } ], "seq_stats": { "molecular_weight": 37920.14720000003, "isoelectric_point": 5.580645561218262 }, "id": "NKIECMJPJD_681", "locus": "GCA000828735_00666" }, { "type": "cds", "sequence": "contig_1", "start": 401422, "stop": 401709, "strand": "-", "frame": 3, "gene": null, "product": "DNA gyrase subunit A", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9XCU2" ], "nt": "ATGAGTGATAATCATCAAAAAATTATTAATACACCTTTAGACAACATTGTTGGTGAAAGTTATGCAAAATATGCAAAATACATTATTCAAGATCGTGCTTTACCTGATATTCGTGATGGTTTAAAACCTGTTCAACGTCGAATTTTATACGCAATGAGTGAATTAGGAATCTTTTATGATAAACCTTATAAAAAATCTGCACGTACAGTAGGAGAAGTAATTGGTAAATATCATCCACACGGAGATTCATCAATTTATGAAGCAATGGTGAGAATGAGTCAAGATTGA", "aa": "MSDNHQKIINTPLDNIVGESYAKYAKYIIQDRALPDIRDGLKPVQRRILYAMSELGIFYDKPYKKSARTVGEVIGKYHPHGDSSIYEAMVRMSQD", "aa_hexdigest": "ea5684a0891746ef6ff46789ec40ca4c", "start_type": "ATG", "rbs_motif": "AGGAG(G)/GGAGG", "psc": { "uniref90_id": "UniRef90_UPI0028540D82", "query_cov": 0.8736842105263158, "subject_cov": 0.83, "identity": 0.6990000000000001, "score": 130.0, "evalue": 1.28e-37, "valid": false, "product": "DNA gyrase subunit A", "uniref50_id": "UniRef50_Q9XCU2" }, "pscc": { "uniref50_id": "UniRef50_Q9XCU2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9XCU2" ], "product": "DNA gyrase subunit A" }, "genes": [], "id": "NKIECMJPJD_682", "locus": "GCA000828735_00667" }, { "type": "cds", "sequence": "contig_1", "start": 401781, "stop": 402497, "strand": "-", "frame": 1, "gene": null, "product": "DNA topoisomerase (ATP-hydrolyzing)", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A971IXM9", "UniRef:UniRef90_A0AA86Z7L8" ], "nt": "TTGGCTGCAAAGCGTGCTCGTGAAGAAACTAAAAAAGTTAAAAAAATTAAAGAAGAACGAGGAATGGGCGGAAAATTAACACCAGCACAAAGTAAAGATCCAACTTTAAATGAATTATTTTTGGTTGAAGGTGATTCAGCTGGTGGATCAGCTAAACTAGGACGAAATAAAAAATACCAGGCTATTTTGCCCTTAAGAGGTAAGGTTCTTAATGTTTTAAAAGCTCGTTTAGTTGATGTTTTAAAAAATGAAGAAATAGCATCAATCTTTACTTGTTTAGGAACAGGAATTGGTGCAGAATTTGATTTAAAAAAATTAAAATATCACAAAATTATTATTATGACCGATGCAGATACCGACGGTAGTCACATCCAAGTTTTATTATTAACTTTATTTTATCGTTTTATGCGGCCTTTAATTGAAAATGGTAATATTTACATAGCTTTGCCTCCACTTTATAAATTAACAAATAAAAATAACAAAAAATTCTTTTATGCTTGGGATGATGTTGAATTAGACCAACTAAAAAAAGAACAAAAAAATTATGAAATCCAACGTTACAAAGGTCTTGGTGAAATGAATGCTGATCAATTATTTGAAACAACAATGGATCCAAACAATCGTTTATTATTAAAAATTAATATTAATGATATTTTACAAGCCGAACGGCAAATCAATACTTTAATGGGTAATGACGTAAGTATTCGAAAGCAGTGA", "aa": "MAAKRAREETKKVKKIKEERGMGGKLTPAQSKDPTLNELFLVEGDSAGGSAKLGRNKKYQAILPLRGKVLNVLKARLVDVLKNEEIASIFTCLGTGIGAEFDLKKLKYHKIIIMTDADTDGSHIQVLLLTLFYRFMRPLIENGNIYIALPPLYKLTNKNNKKFFYAWDDVELDQLKKEQKNYEIQRYKGLGEMNADQLFETTMDPNNRLLLKININDILQAERQINTLMGNDVSIRKQ", "aa_hexdigest": "4af84aae3bb972526f297e447ed66d91", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0AA86Z7L8", "query_cov": 0.9117647058823529, "subject_cov": 0.9079497907949791, "identity": 0.963, "score": 414.0, "evalue": 1.52e-145, "valid": true, "product": "DNA topoisomerase (ATP-hydrolyzing)", "uniref50_id": "UniRef50_A0A971IXM9" }, "pscc": { "uniref50_id": "UniRef50_A0A971IXM9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A971IXM9" ], "product": "DNA topoisomerase (ATP-hydrolyzing)" }, "genes": [], "id": "NKIECMJPJD_683", "locus": "GCA000828735_00668" }, { "type": "cds", "sequence": "contig_1", "start": 402564, "stop": 402854, "strand": "-", "frame": 1, "gene": null, "product": "DNA topoisomerase (ATP-hydrolyzing)", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AA87CUZ8", "UniRef:UniRef90_A0AA87CUZ8" ], "nt": "ATGGTTTCATTTGCTAATTCTGTGAAAACTAATGAAGGCGGTGTACATGAAAATGCCTTTAAAAATGCTTTAACAAGTGTTGTTAATAATTACGCAAGAAAACATAATTTACTTAAGGAAAAAGATAAAAATTTAGAAGGTGATGATATACGTGAGGGTTTATCAAGTGTAATTTCACTTCGAATCCCCGAAAGCTTAATTAGCTATGAGGGTCAAACTAAAAACAAATTATTCACACCAGAAGCAAATGAAGCAGTAAAAAAAACAATTGAAGATAACTTTAGTTTTTGA", "aa": "MVSFANSVKTNEGGVHENAFKNALTSVVNNYARKHNLLKEKDKNLEGDDIREGLSSVISLRIPESLISYEGQTKNKLFTPEANEAVKKTIEDNFSF", "aa_hexdigest": "61e2403873329b64d3e765f7e41902e8", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0AA87CUZ8", "query_cov": 0.9166666666666666, "subject_cov": 0.9072164948453608, "identity": 0.9890000000000001, "score": 167.0, "evalue": 1.97e-52, "valid": true, "product": "DNA topoisomerase (ATP-hydrolyzing)", "uniref50_id": "UniRef50_A0AA87CUZ8" }, "pscc": { "uniref50_id": "UniRef50_A0AA87CUZ8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AA87CUZ8" ], "product": "DNA topoisomerase (ATP-hydrolyzing)" }, "genes": [], "id": "NKIECMJPJD_684", "locus": "GCA000828735_00669" }, { "type": "cds", "sequence": "contig_1", "start": 403260, "stop": 403493, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02518.31" ], "nt": "ATGAATGCTAACGCAAAAAACATTAATGTTATTTTGCATGAAGATAATTCAATTAGTGTTTTAGATGATGGGCGTGGTATACCAGTTGACATTAATTCTCAAACAAAAATTTCTACTGTTGAAACTGTTCTAACAGTTTTGCATGCTGGAGGTAAGTTTGATGAAAGTGCATACAAAACTGCTGGTGGTTTACATGGTGTTGGTTCATCAGTAGTTAATGCTTTAAGTTCTTGA", "aa": "MNANAKNINVILHEDNSISVLDDGRGIPVDINSQTKISTVETVLTVLHAGGKFDESAYKTAGGLHGVGSSVVNALSS", "aa_hexdigest": "ef9bf87c2282da8228433799e10a423d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02518.31", "name": "HATPase_c", "length": 76, "aa_cov": 0.948051948051948, "hmm_cov": 0.8157894736842105, "evalue": 2.1443526594276934e-09, "score": 33.37578201293945, "start": 4, "stop": 76 } ], "seq_stats": { "molecular_weight": 7948.7559, "isoelectric_point": 5.349140357971191 }, "id": "NKIECMJPJD_685", "locus": "GCA000828735_00670" }, { "type": "cds", "sequence": "contig_1", "start": 403524, "stop": 403643, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAAATAAATACGATGGGAATGCTATTAAAATCTTAGAAGGGTTGGAAGCTGTTCGTAAGCGTCCTGGAATGTATATTGGCTCTACAAGTAGCACTGGATTACATCATTTAATTTGA", "aa": "MANKYDGNAIKILEGLEAVRKRPGMYIGSTSSTGLHHLI", "aa_hexdigest": "cc63cf1ba3b673bd2d1eadb1783cf925", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4242.878099999999, "isoelectric_point": 9.397830390930174 }, "id": "NKIECMJPJD_686", "locus": "GCA000828735_00671" }, { "type": "cds", "sequence": "contig_1", "start": 404001, "stop": 404093, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTCCGTTATATTGATGTTTGTTATCATAATCAAGCCAAAAAATATAAAATTCGTTCAGGAATTGCAATCGCTGCAAATCAAGTTGGTTGA", "aa": "MVRYIDVCYHNQAKKYKIRSGIAIAANQVG", "aa_hexdigest": "76299d926cea718333e551350547782c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3410.9673000000003, "isoelectric_point": 9.744928169250485 }, "id": "NKIECMJPJD_687", "locus": "GCA000828735_00672" }, { "type": "cds", "sequence": "contig_1", "start": 404495, "stop": 404698, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTGATAACCAAGAAATAATAGAATCTATTTTACAAGCAGATAACGCAAAAGGTGGTAATGGCAACTTTTTAATGATACTAATCATTATCATTCCAATTGTATTAGTTGTAGTATTTATTATGCGTAAAAAACGTAAACAAAATGGTGAAAATCAAACTAGTGTTAATAACAAAGATAAGTCAGATACAAATGAAGTTTGA", "aa": "MLDNQEIIESILQADNAKGGNGNFLMILIIIIPIVLVVVFIMRKKRKQNGENQTSVNNKDKSDTNEV", "aa_hexdigest": "4b6a2a88e06275280f451890e24368c6", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7526.667800000003, "isoelectric_point": 6.125674629211424 }, "id": "NKIECMJPJD_688", "locus": "GCA000828735_00673" }, { "type": "cds", "sequence": "contig_1", "start": 404798, "stop": 405091, "strand": "+", "frame": 2, "gene": null, "product": "DUF5385 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2C9DYK1", "UniRef:UniRef90_UPI00290991E4" ], "nt": "ATGACAAAACAACAAAGAATTGATTATAAAAATGAACAAAAAGCAATCAAAACACTTAAAACAACGAATCCAGAGCAATATAAAATTGAAATGGAACGAATTAAAAAAGAAAAACGTGCTAAACCTAAAGAACTATATGTAGTTTTGTTTACTACAAGAAATGCTAAAACTTTAATTATAGATGAACCTCGTGCGATTGAGTGTGAAGTTCGTTTGGTAAAAATTAATAAAAAAGAAAACCGACGCGAAATTGATGTTGTGCGTGCTTTAGATTATGAAGAAGAAATGTTATGA", "aa": "MTKQQRIDYKNEQKAIKTLKTTNPEQYKIEMERIKKEKRAKPKELYVVLFTTRNAKTLIIDEPRAIECEVRLVKINKKENRREIDVVRALDYEEEML", "aa_hexdigest": "cb925ef91c8b59ce88ce616e4037ed0b", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 404549, "stop": 405211, "observations": { "mutation": [ 404696, 405089 ], "stop": [ 404696, 405089 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00290991E4", "query_cov": 1.0, "subject_cov": 0.4092827004219409, "identity": 1.0, "score": 180.0, "evalue": 2.18e-55, "gene_start": 1, "gene_end": 97, "reference_start": 101, "reference_end": 197, "reference_sequence": "MGVRGWGLGECFSGVSDGGNGNFLMILIIIIPIVLVVVFIMRKKRKQNGENQTSVNNKDKSDTNEVWTTIKKYLRSIDDKGKEVIDSYVVKRAEPHNLAQMTKQQRIDYKNEQKAIKTLKTTNPEQYKIEMERIKKEKRAKPKELYVVLFTTRNAKTLIIDEPRAIECEVRLVKINKKENRREIDVVRALDYEEEMLWIEPIKAKDDEIYNKRLEADKKKQQKAAQRRQKQLEKQKK" }, "paralog": false, "identity": 0.990909090909091, "score": 377.0, "evalue": 2.17e-135, "description": "Internal stop codon at 404696, 405089. Nonsense mutation around 404696, 405089.", "psc": { "uniref90_id": "UniRef90_UPI00290991E4", "product": "DUF5385 domain-containing protein", "uniref50_id": "UniRef50_A0A2C9DYK1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00290991E4", "UniRef:UniRef50_A0A2C9DYK1" ] }, "pscc": { "uniref50_id": "UniRef50_A0A2C9DYK1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2C9DYK1" ] } }, "id": "NKIECMJPJD_689", "locus": "GCA000828735_00674" }, { "type": "cds", "sequence": "contig_1", "start": 405295, "stop": 406212, "strand": "+", "frame": 1, "gene": "fmt", "product": "methionyl-tRNA formyltransferase", "db_xrefs": [ "COG:COG0223", "COG:J", "EC:2.1.2.9", "GO:0004479", "GO:0005737", "GO:0005829", "GO:0071951", "RefSeq:WP_006688740.1", "SO:0001217", "UniParc:UPI000169E234", "UniRef:UniRef100_A0AAC9X757", "UniRef:UniRef50_Q9PQ27", "UniRef:UniRef90_Q9PQ27" ], "nt": "ATGAAATATAAAGTAATGTTTTTTGGTACACCAGAAATCGCTAAAATTGTTTTGGAAACATTATTTAACATGCATGAAGTTGATTTAATCGCTGTTGTTAGTCAACCAGATGCACATTTTGATCGAAAAAAAAATGTCGTCTACTCTCCTGTTAAACAGTTTTGTTTAGATCATAATATTAAGTTATTTCAACCAGAAAAAATCAAAGAAATTGAAGAAGAAATCCGTATTTTAGGACCAGATATTATCATTACTTGTGCTTTTGGTCAATTTATTAATCAAGGGATAATTGATATTCCTAAATACAAAATTGTTAATATTCATGCTTCACTTTTACCAAAATTACGTGGCGGAGCACCAATTCATTATGCCATTTTAAATGGTGAATTAAAAACAGGAATCACTTTAATGCATACGATTAAAAAAATGGATGCAGGTAATATTTTATTTCAAAGAAGTTTAGAAATAAATGATTGTACAACAACTAAATCATTAACTTTAGAGTTAGCTAATCTTAGTGCGTTGATGATTAAAGAACATTTTTTAGAATTAGTAAAACCAAATTTAGTTGGAATTCAACAAGATGAAAATTCTGTTAGTTTTGCTTATAATATTCAAAAGGATCAAAATATTATTAATTTTGACCAACCAGCTTTTTTTATTAATCGTTTTGTTAATGCTATGTATGATAAACCAATCGCTATTATGCAATATAATGGCGTTTCAATTAAAGTATATCAAGTTAAAATCACTAATCAAAAGTCATGTCAAAAACCTGGAACAATCGTGATTTTTAAAAATCAACTTTTTGTAAGTACGCAAGATTTTGATATTGAACTATTATTGATTCAACTTCCAAATAAAAAACCTTTATCTCCAAAAGTTTTATTGAATGGGAGAAACCCTTTTATTAATTAA", "aa": "MKYKVMFFGTPEIAKIVLETLFNMHEVDLIAVVSQPDAHFDRKKNVVYSPVKQFCLDHNIKLFQPEKIKEIEEEIRILGPDIIITCAFGQFINQGIIDIPKYKIVNIHASLLPKLRGGAPIHYAILNGELKTGITLMHTIKKMDAGNILFQRSLEINDCTTTKSLTLELANLSALMIKEHFLELVKPNLVGIQQDENSVSFAYNIQKDQNIINFDQPAFFINRFVNAMYDKPIAIMQYNGVSIKVYQVKITNQKSCQKPGTIVIFKNQLFVSTQDFDIELLLIQLPNKKPLSPKVLLNGRNPFIN", "aa_hexdigest": "7d9f1f67886859c36c95ff7041299845", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI000169E234", "ncbi_nrp_id": "WP_006688740.1", "uniref100_id": "UniRef100_A0AAC9X757", "db_xrefs": [ "SO:0001217", "UniParc:UPI000169E234", "RefSeq:WP_006688740.1", "UniRef:UniRef100_A0AAC9X757" ] }, "ips": { "uniref100_id": "UniRef100_A0AAC9X757", "uniref90_id": "UniRef90_Q9PQ27" }, "psc": { "uniref90_id": "UniRef90_Q9PQ27", "gene": "fmt", "product": "methionyl-tRNA formyltransferase", "ec_ids": [ "2.1.2.9" ], "uniref50_id": "UniRef50_Q9PQ27", "cog_id": "COG0223", "cog_category": "J", "go_ids": [ "GO:0004479", "GO:0005737", "GO:0005829", "GO:0071951" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQ27", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQ27" ], "product": "Methionyl-tRNA formyltransferase" }, "genes": [ "fmt" ], "id": "NKIECMJPJD_690", "locus": "GCA000828735_00675" }, { "type": "cds", "sequence": "contig_1", "start": 406396, "stop": 406512, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTGTTTTTTTTATTTTATATGGTGAAATCAATCTTTTAGGTTTTAATGGAATAATTGGTGTTAATGGTCGTTGGTATGGAGAAGGTTTTAAATTAGATGAAAACCAACAATGA", "aa": "MIVFFILYGEINLLGFNGIIGVNGRWYGEGFKLDENQQ", "aa_hexdigest": "f47de34759327f0c4362dc4dc432e99e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4365.958500000002, "isoelectric_point": 4.406579399108887 }, "id": "NKIECMJPJD_691", "locus": "GCA000828735_00676" }, { "type": "cds", "sequence": "contig_1", "start": 406588, "stop": 406686, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATAGAAAAAATGCAGGCATTAGTTAATAAAATTACTAATCAATACAATCATGGTATTGAATTTGGTACAAATATAATTAGTTCATATCCAGTTTGA", "aa": "MIEKMQALVNKITNQYNHGIEFGTNIISSYPV", "aa_hexdigest": "df9b88035acfe7dc86daa5598f25fdff", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3654.1739000000016, "isoelectric_point": 6.510531425476074 }, "id": "NKIECMJPJD_692", "locus": "GCA000828735_00677" }, { "type": "cds", "sequence": "contig_1", "start": 406638, "stop": 406769, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCTCAACGAATCTTTGGCGTACTAAAAGCAAATATAATACATACAATAACTGCTAATCCTAAAGTTGAAAACATTGCAACTCAAACTGGATATGAACTAATTATATTTGTACCAAATTCAATACCATGA", "aa": "MSQRIFGVLKANIIHTITANPKVENIATQTGYELIIFVPNSIP", "aa_hexdigest": "a8bbf60077c5f099ccdc23805294220a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4753.5196, "isoelectric_point": 8.27550029754639 }, "id": "NKIECMJPJD_693", "locus": "GCA000828735_00678" }, { "type": "cds", "sequence": "contig_1", "start": 406903, "stop": 407097, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAAAAATTACAAATGCTATTATGAATCGCTCAAAACATTTTGAGACATATGCTGCAGATTTATACAATGAACTTAAAGATTCAGAACAATATTATAATGAATTTAACAAAAAACGTTTAGAATTAAAAAAGCGTAATAAAGCGAATGAACGAATAAAATATAAAATTGAAGATGATCAAAAAGGAAAATAA", "aa": "MTKITNAIMNRSKHFETYAADLYNELKDSEQYYNEFNKKRLELKKRNKANERIKYKIEDDQKGK", "aa_hexdigest": "102b1be6e4fdca714e7b3f9f6d3ce8e5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7833.7847999999985, "isoelectric_point": 9.486925697326662 }, "id": "NKIECMJPJD_694", "locus": "GCA000828735_00679" }, { "type": "cds", "sequence": "contig_1", "start": 407367, "stop": 408152, "strand": "+", "frame": 3, "gene": "obgE", "product": "GTPase ObgE", "db_xrefs": [ "COG:COG0536", "COG:DL", "EC:3.6.5.-", "GO:0000287", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0042254", "SO:0001217", "UniRef:UniRef50_Q9PQ29", "UniRef:UniRef90_Q9PQ29" ], "nt": "GTGCCACTAGGAACAGTTGTTTATAATCCTATCACTAATGAAATTTTGGCAGATATTAATATTGATCAACAAAAATATTTAGTTGCCCAAGGTGGTTTAGGCGGTCATGGAAATACACATTTTAAATCGCCTTTTAATAAAGCACCAAATTTATATGAATTAGGAGAATTAGGTGAAAATGTTGAAGTTTTATTAGAATTAAAAACTATTGCTGATATTGGAATTATAGGATTACCAAATGCAGGAAAATCAACATTAATTTCTACATTTACAAATGCTAAACCAAAAACGGCAAATTACATGTTTACAACTTTAAATCCCGTTTTAGGTACAATTTATCGTGATCAAAATCGTATCATCTTTGCGGATATTCCTGGTTTAATTGAAGGAGCACATACAGGAGTTGGCTTGGGGCATGATTTTTTAAAACATATCGAACGTTGTTTTTTATTAATTCATTTAATTTCTTTAGATCCTAATGATAATCCTGATATTATTAATGCTTATGAAACGATTGTTAATGAATTAAAACAATATAAACAAAATTTAGTTAACAAACCAATTGTATTGGTTGCTAATAAAATAGATCAAATTGGAGCATTAGAAAATTTGCAGATTCTAAAAGAATATTTAAAAAATAATCAAGAAATTAAAATTATATCTGCGCTAACAAATTTACATGTAGATAATATGTTAGATGATGTTATTAAAATTTACTTTGCACAAAAAAAGATTTATGAGCAACGACTTAAAGAACAATTACCAGTTGATCAAATTTTAAAATGA", "aa": "MPLGTVVYNPITNEILADINIDQQKYLVAQGGLGGHGNTHFKSPFNKAPNLYELGELGENVEVLLELKTIADIGIIGLPNAGKSTLISTFTNAKPKTANYMFTTLNPVLGTIYRDQNRIIFADIPGLIEGAHTGVGLGHDFLKHIERCFLLIHLISLDPNDNPDIINAYETIVNELKQYKQNLVNKPIVLVANKIDQIGALENLQILKEYLKNNQEIKIISALTNLHVDNMLDDVIKIYFAQKKIYEQRLKEQLPVDQILK", "aa_hexdigest": "035d742d431377a115ecb2e19057981b", "start_type": "GTG", "rbs_motif": null, "genes": [ "obgE" ], "truncated": "both", "pseudogene": { "start": 407100, "stop": 408407, "observations": { "mutation": [ 407166, 407235, 408150, 408252 ], "stop": [ 407166, 407235, 408150, 408252 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQ29", "query_cov": 1.0, "subject_cov": 0.6, "identity": 0.996, "score": 516.0, "evalue": 1.86e-182, "gene_start": 1, "gene_end": 261, "reference_start": 90, "reference_end": 350, "reference_sequence": "MAFIDKCKIVLIAGNGGDGIVSWRRETHVPEGGPAGGNGGNGGSIWFVGNHNETSLEFLKYKKIIRAKHGEKGDIKNQHGANAEDVFINVPLGTVVYNPITNEILADINIDQQKYLVAQGGLGGHGNTHFKSPFNKAPNLYELGELGENVEVLLELKTIADIGIIGLPNAGKSTLISTFTNAKPKTANYMFTTLNPVLGTIYRDQNRIIFADIPGLIEGAHTGVGLGHDFLKHIERCFLLIHLISLDPNDNPDIINAYETIVNELKQYKQNLVNKPIVLVANKIDQIGALENLQILKEYLKNNQEIKIISALTNLHVDNMLDDVIKIYFAQKKIYEQRLKEQLPVDQILKWTSDIPKSKELDKTIEIIKVDDHIFEVFGEYLKYWAHRIPLKTQDNLIRFNQKLQSINFNQQLLQAGAVAGDSIKIYDITLEFEE" }, "paralog": false, "identity": 0.9885057471264368, "score": 851.0, "evalue": 4.72e-317, "description": "Internal stop codon at 407166, 407235, 408150, 408252. Nonsense mutation around 407166, 407235, 408150, 408252.", "psc": { "uniref90_id": "UniRef90_Q9PQ29", "gene": "obgE", "product": "GTPase ObgE", "ec_ids": [ "3.6.5.-" ], "uniref50_id": "UniRef50_Q9PQ29", "cog_id": "COG0536", "cog_category": "DL", "go_ids": [ "GO:0000287", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0042254" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQ29", "EC:3.6.5.-", "UniRef:UniRef50_Q9PQ29", "COG:COG0536", "COG:DL", "GO:0000287", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0042254" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQ29", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQ29" ], "product": "GTPase Obg" } }, "id": "NKIECMJPJD_695", "locus": "GCA000828735_00680" }, { "type": "cds", "sequence": "contig_1", "start": 408415, "stop": 409182, "strand": "+", "frame": 1, "gene": "nadE", "product": "NH(3)-dependent NAD(+) synthetase", "db_xrefs": [ "COG:COG0171", "COG:H", "RefSeq:WP_006689045.1", "SO:0001217", "UniParc:UPI00000C1C77", "UniRef:UniRef100_Q9PQ30", "UniRef:UniRef50_Q9PQ30", "UniRef:UniRef90_Q9PQ30" ], "nt": "ATGAATAATGATTTGATTAAATATCTACAATGGTTGAAAAATGATTTTATAAAATTTTTAAATGACGCACAAACGAATAATGTTATTTTGGGCATTTCAGGTGGTATTGATAGCACTTTAACATTAGCTATTTTAAATGATTTAAAAAAAGAGTATGATCTTAATATTTATGCTTATTTTTTAGATATTCATAATTCGAGTTTAGATTATGAATGTATTAATGAATTAAAAAATATTTATCCTAACATTGAAGTTATCAATCTTGTTGATATCTATGATAGCTATTGTAAAATGATTAATAAAAAAACAAATGATAAATATGTTCTTTATAATTTAAAGCCTAAAATTCGTACAAATTATTTATATGCAATGGCGAATGCATACAAGGGTGTTGTTGTGAGTAATTTAAACTATGATGAGTACATTTTAGGTTTTTTTACTAAATATGGCGATAGTGCCGCTGATTATTATATGTTAATTGGTTTACTAAAAAAACATATTTATGAATTAGGTGCTTATTATCATTTACCAAATAAAATTTTAAACCGTGCGCCTACTCCAGCAAATGAAGATGATGAACACAAAACTGATGAGAGTTTTTTTGGTTTTACATATAACGATCTTGATCAATTTTTACTTTATCGTAAAATTAATCCTAAAATTGTTAGCATGATTAAAAAACGTTATGAGACTAATGCTCATAAACATTTTGTTTTTGATAAAAAGAAATTATTTTTAAATTATAAATTAGGAAATAAACATGAATAA", "aa": "MNNDLIKYLQWLKNDFIKFLNDAQTNNVILGISGGIDSTLTLAILNDLKKEYDLNIYAYFLDIHNSSLDYECINELKNIYPNIEVINLVDIYDSYCKMINKKTNDKYVLYNLKPKIRTNYLYAMANAYKGVVVSNLNYDEYILGFFTKYGDSAADYYMLIGLLKKHIYELGAYYHLPNKILNRAPTPANEDDEHKTDESFFGFTYNDLDQFLLYRKINPKIVSMIKKRYETNAHKHFVFDKKKLFLNYKLGNKHE", "aa_hexdigest": "8a5ed024897ce9cc5ce2bb20a55110fa", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI00000C1C77", "ncbi_nrp_id": "WP_006689045.1", "uniref100_id": "UniRef100_Q9PQ30", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1C77", "RefSeq:WP_006689045.1", "UniRef:UniRef100_Q9PQ30" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQ30", "uniref90_id": "UniRef90_Q9PQ30" }, "psc": { "uniref90_id": "UniRef90_Q9PQ30", "gene": "NadE", "product": "NH(3)-dependent NAD(+) synthetase", "uniref50_id": "UniRef50_Q9PQ30", "cog_id": "COG0171", "cog_category": "H" }, "pscc": { "uniref50_id": "UniRef50_Q9PQ30", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQ30" ], "product": "NH(3)-dependent NAD(+) synthetase" }, "genes": [ "nadE" ], "id": "NKIECMJPJD_696", "locus": "GCA000828735_00681" }, { "type": "cds", "sequence": "contig_1", "start": 409175, "stop": 409291, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAATAAAAAAGGTAAAAAATTTTATGATTTTTTAAAAACAATCGAGGGTTTTAAAATTATTACGATTATTTCATATGTTTTTGTAGCAATTTTATTAGCAATTTTAATCTGA", "aa": "MNNKKGKKFYDFLKTIEGFKIITIISYVFVAILLAILI", "aa_hexdigest": "c8e9157b3cd742ff30ea25d516f2980a", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4407.3899999999985, "isoelectric_point": 9.778516197204588 }, "id": "NKIECMJPJD_697", "locus": "GCA000828735_00682" }, { "type": "cds", "sequence": "contig_1", "start": 409652, "stop": 409906, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATGTTATTAATCTAATTAAAAATTTAAAACAAATTTTACGAACAGCAAATAATGAACGTCATTTGATTGAATTAAAAAATATTTTTGTTAAACAACATTTATTACCACTATATGATGAATTAAAAAAAAGCGCTAATAAAAAAGAAATGGGTTTATTAATTAATAATTTCAAACAACAAATTGAATTTGTTACTGATCAAATTTTAAAAGAATTAAATGATAAAGATGATCAAGTTGATTTAAAAAAATGA", "aa": "MDVINLIKNLKQILRTANNERHLIELKNIFVKQHLLPLYDELKKSANKKEMGLLINNFKQQIEFVTDQILKELNDKDDQVDLKK", "aa_hexdigest": "ff00212cf1918553240dc94b37b96c90", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9985.584799999993, "isoelectric_point": 8.892462730407715 }, "id": "NKIECMJPJD_698", "locus": "GCA000828735_00683" }, { "type": "cds", "sequence": "contig_1", "start": 409919, "stop": 410278, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01409.25" ], "nt": "TTGTTTGCTCCTTTTATTAATAATGGGCATCATCATATTCTCAATAGTATTATTGATGATATTGCATCTTTTTTTAAAAAATTAAATTTTGAAATTGTAAGTGGTTCAGAAGTTGTTTCACCTATTTATAATTTTGATCATTTAAATATTGATGAAAATCATCCAGCAAGAGCAAGTGCTGATTCTTTTTTTATTAATTCAATTAAAATGTTACGTACGCATTGCACAACTACAACCGCACAATTTTTAGAAAATAACGTAAACAAGGACATTAGAATTATGTCTTTTGGTAATGTTTATCGAAAAGATGATGATGATGCAACACATAGCCACCAATTCAATCAAGTTGATTTTGTATGA", "aa": "MFAPFINNGHHHILNSIIDDIASFFKKLNFEIVSGSEVVSPIYNFDHLNIDENHPARASADSFFINSIKMLRTHCTTTTAQFLENNVNKDIRIMSFGNVYRKDDDDATHSHQFNQVDFV", "aa_hexdigest": "8f7e10288f831c7756f96bb3c8527195", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01409.25", "name": "tRNA-synt_2d", "length": 123, "aa_cov": 0.9747899159663865, "hmm_cov": 1.0, "evalue": 1.4426155148203387e-38, "score": 128.2692413330078, "start": 4, "stop": 119 } ], "seq_stats": { "molecular_weight": 13675.073099999983, "isoelectric_point": 5.635040473937988 }, "id": "NKIECMJPJD_699", "locus": "GCA000828735_00684" }, { "type": "cds", "sequence": "contig_1", "start": 410492, "stop": 410653, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01409.25" ], "nt": "ATGCTACATGAAAATGTTCTAAAAGCTGCGAATATTAACGATATTAGAACAGGGATGGCTTTTGGTGTGGGTATTGATCGAATTGCAATGTTAAAATATGAAATTGATGATATTCGTTATTTATATTCAAATAATTTTAAATTTAATGCCCAAATTAAATAA", "aa": "MLHENVLKAANINDIRTGMAFGVGIDRIAMLKYEIDDIRYLYSNNFKFNAQIK", "aa_hexdigest": "abb01eab37d281a2739143d251a43dce", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01409.25", "name": "tRNA-synt_2d", "length": 51, "aa_cov": 0.9622641509433962, "hmm_cov": 1.0, "evalue": 3.7282181727737563e-19, "score": 64.69293975830078, "start": 1, "stop": 51 } ], "seq_stats": { "molecular_weight": 6150.072499999999, "isoelectric_point": 8.085383033752443 }, "id": "NKIECMJPJD_700", "locus": "GCA000828735_00685" }, { "type": "cds", "sequence": "contig_1", "start": 410685, "stop": 410951, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01588.26" ], "nt": "ATGATTTTAAGTTTAAATTTATTACATAAGATTAGTCCAAAACTAAAAAAGATTCCTTTAAATCAATTGTGCACAGCTTTAATGGATTTAGGTTGTGAAGTTGAAACAATTAATAGCATTAAACCATCTACTAATTTAGTTTTTGCTAAAGTATTAGAAAAAACTAGACATCCTAATGCTAATCATTTAAATTTAGTTAAAGTTAAAGCAAACCAAAAAGTTTATGAGATTGTTTGTGGTGCAAATAATTTTAGTGTTCATAATTGA", "aa": "MILSLNLLHKISPKLKKIPLNQLCTALMDLGCEVETINSIKPSTNLVFAKVLEKTRHPNANHLNLVKVKANQKVYEIVCGANNFSVHN", "aa_hexdigest": "2672d3ffc63b3ab1f196bcc06318c1c8", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01588.26", "name": "tRNA_bind", "length": 41, "aa_cov": 0.45454545454545453, "hmm_cov": 1.0, "evalue": 3.588484340204411e-08, "score": 29.026796340942383, "start": 46, "stop": 85 } ], "seq_stats": { "molecular_weight": 9817.550499999996, "isoelectric_point": 9.513357734680177 }, "id": "NKIECMJPJD_701", "locus": "GCA000828735_00686" }, { "type": "cds", "sequence": "contig_1", "start": 411036, "stop": 411227, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTATGTGCTTACAGTGAAATTAATCCACAAGCTACTAGTTTTTTAACATCAACAGATTTAGATGGAATTTTAGTTTTAGATGACAATTATGACCATTATAAAACCCCAAATCAAATTTTTAATTTAGATGATGTTATTTTAGATTTATCAATTCCATCAAATCGGAATGATTTAAATGGTTATTTTTGA", "aa": "MLCAYSEINPQATSFLTSTDLDGILVLDDNYDHYKTPNQIFNLDDVILDLSIPSNRNDLNGYF", "aa_hexdigest": "657086520ff1e5595b1c739210a070e8", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7157.817400000002, "isoelectric_point": 4.0500284194946286 }, "id": "NKIECMJPJD_702", "locus": "GCA000828735_00687" }, { "type": "cds", "sequence": "contig_1", "start": 411315, "stop": 413003, "strand": "+", "frame": 3, "gene": "pheT", "product": "phenylalanine--tRNA ligase subunit beta", "db_xrefs": [ "COG:COG0073", "COG:J", "EC:6.1.1.20", "GO:0000049", "GO:0000287", "GO:0004826", "GO:0005524", "GO:0006432", "GO:0009328", "KEGG:K01890", "SO:0001217", "UniRef:UniRef50_Q9PQ33", "UniRef:UniRef90_Q9PQ33" ], "nt": "GTGCGGATTTTATCTGATGATGTTAATTCGTATGGAATAATCGAGGTTAAAAATATTCAAAATTATATTTTAAAATGGAATACTAAAAGTATATTGATTAATAATCAAATTAAAGTTCTTAATAATTTCGCTGATAATATGAATTTTTTAACATTATTAACTGCTAATCCACTTCATGCATTTGATGCACGAAAAATTAGTGGTCAAATTGTTGTTAAAAATGCTGAAGAAGATGCAATTTTATTGGGTTTAGATCAAAAAGAATATTTAATTAAAAAAGGGGATCTAATTATCGTTGATGATCAAAAAATTTTAGCACTTGCTGGAATTATTGGTTCTAATGATTCTAAAATTGATGCAACAAGCACAACTGCTTATATTGAATGTGCAAATTTTAATCCTATGTTGATTGCAAATACAGCGCGTCGTTTAAAAATCAATACCGCAGCGGCTATGCGTTTTAGCAAACCCTTAACTAATTATGTTACAAAAGTAACATTAAAAAAATTGTTAGCTAATTTTAAATCAGATGCAAAATTAATTTATTATTTTAAACATTCAGTTCATAATGTCATTAAAAATAAAATTAATCAAGTAAGTGATTTTGTGGGTGCAGAAATTGATTTAGACACTGCTCAAACATTTTTAAAACGCTTAGGTTATAAAATTAATAAGTCTAATTTAATTACTCCAAGTCATCGATATGATGTATTAAATGAGTTTGATGTTTATGAAGATATTATGAAAAAAATTAGTATTCAAGAAATCAAACCACAACCAATCAGTTTTGATATTTTAAATTTTGAAAATAATCTAGCTTATGATTTTGAAAAAAAAGTTAGTGATTTTTTAGTTGATCAAGGTTTGTTTGAGTGTAAAACTTATAATTTAAAAAATCAAACACAAGCACATGAGTTTAATTTTTTTAATTTTAAACAAGCTTATGAAATTAATAATCCAATTTCAAATATGCGTTCGCATTTAAAATTAAATAACCTTAACTCCTTACTTGAAGTATTAGAATATAACCAAAACCAAAAAAATGAATTAGAAAATATCTTTGAGATTTCAAAGATTAATCCAGTTGATTCAAGTCAACAAACAGTTTTATCAATCATTTTATGTAAACCATTAATTAATTCAAAAATTAATGATTCATTAATAGTTAATAATTTTGTGACAACAAAAGCTTTACTACATGCTTTATTAACAAAATTAAATATTGATTATGCATATGATAAAAATCATGTTGTTAATGAATTATATGATAATAACCAATTAGCTTTAATTAATGCAAACAAACAAGTATTTGGTTTTATTGGTCAACTAAAAAATCAAGTCAAAAAAACATATGGTTTAAGTAATGATATTTTTATTATTAATTTAAATTTAACTAGTTATTTAAATCAAAAACAAGTAATCACTAAAGTTATTAAGCCAAGTATGTATCATGATGTCATACGTGATATTAGTGTTAAATTAGCTTCTGACGTTGACTTAAATAATATAATTACTAATATTAAAAAAATTAAAAATATTAGAAAAGTTGAAATCAGCGATCTTTATATAAAAGATGATGGAATTATTTATACTTTTAAATATTATATTAATGATCATTTATCTAATTTAAGTAGTGAACAAATTATAATCATTGAACAAGAAGTAAATAATTATTTGAAACAATTTTAA", "aa": "MRILSDDVNSYGIIEVKNIQNYILKWNTKSILINNQIKVLNNFADNMNFLTLLTANPLHAFDARKISGQIVVKNAEEDAILLGLDQKEYLIKKGDLIIVDDQKILALAGIIGSNDSKIDATSTTAYIECANFNPMLIANTARRLKINTAAAMRFSKPLTNYVTKVTLKKLLANFKSDAKLIYYFKHSVHNVIKNKINQVSDFVGAEIDLDTAQTFLKRLGYKINKSNLITPSHRYDVLNEFDVYEDIMKKISIQEIKPQPISFDILNFENNLAYDFEKKVSDFLVDQGLFECKTYNLKNQTQAHEFNFFNFKQAYEINNPISNMRSHLKLNNLNSLLEVLEYNQNQKNELENIFEISKINPVDSSQQTVLSIILCKPLINSKINDSLIVNNFVTTKALLHALLTKLNIDYAYDKNHVVNELYDNNQLALINANKQVFGFIGQLKNQVKKTYGLSNDIFIINLNLTSYLNQKQVITKVIKPSMYHDVIRDISVKLASDVDLNNIITNIKKIKNIRKVEISDLYIKDDGIIYTFKYYINDHLSNLSSEQIIIIEQEVNNYLKQF", "aa_hexdigest": "aabf00c23dab1d26fa4abdc5438e2345", "start_type": "GTG", "rbs_motif": null, "genes": [ "pheT" ], "truncated": "5-prime", "pseudogene": { "start": 411015, "stop": 413003, "observations": { "mutation": [ 411225 ], "stop": [ 411225 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQ33", "query_cov": 1.0, "subject_cov": 0.727979274611399, "identity": 0.995, "score": 1074.0, "evalue": 0.0, "gene_start": 1, "gene_end": 562, "reference_start": 211, "reference_end": 772, "reference_sequence": "MILSLNLLHKISPKLKKIPLNELCAALMDLGCEVETINSIKPSTNLVFAKVLEKTRHPNANHLNLVKVKANQKVYEIVCGANNFSVHNWVVLAKLNAELANGLKITPRELRGYVSNGMLCAYSEINPQATSFLTSTDLDGILVLDDNYDHYKTPNQIFNLDDVILDLSIPSNRNDLNGYFWIAKELCAYFDFEYVVDATINHRPHKEIIDVRILSDDVNSYGIIEVKNIQNYILKWNTKSILINNQIKVLNNFADNMNFLTLLTANPLHAFDARKISGQIVVKNAEEDAILLGLDQKEYLIKKGDLIIVDDQKILALAGIIGSNDSKIDATSTTAYIECANFNPMLIANTARRLKINTAAAMRFSKPLTNYVTKVTLKKLLANFKSDAKLIYYFKHSVHNVIKNKINQVSDFVGAEIDLDTAQTFLKRLGYKINKSNLITPSHRYDVLNEFDVYEDIMKKISIQEIKPQPISFDILNFENNLAYDFEKKVSDFLVDQGLFECKTYNLKNQTQAHEFNFFNFKQAYEINNPTSNMRSHLKLNNLNSLLEVLEYNQNQKNELENIFEISKINPVDSSQQTVLSIILCKPLINSKINDSLIVNNFVTTKALLHALLTKLNIDYAYDKNHVVNELYDNNQLALINANKQVFGFIGQLKNQVKKTYGLSNDIFIINLNLTSYLNQKQVITKVIKPSMYHDVIRDISVKLASDVDLNNIIANIKKIKNIRKVEISDLYIKDDGIIYTFKYYINDHLSNLSSEQIIIIEQEVNNYLKQF" }, "paralog": false, "identity": 0.9954682779456193, "score": 1298.0, "evalue": 0.0, "description": "Internal stop codon at 411225. Nonsense mutation around 411225.", "psc": { "uniref90_id": "UniRef90_Q9PQ33", "gene": "pheT", "product": "phenylalanine--tRNA ligase subunit beta", "ec_ids": [ "6.1.1.20" ], "uniref50_id": "UniRef50_Q9PQ33", "cog_id": "COG0073", "cog_category": "J", "kegg_orthology_id": "K01890", "go_ids": [ "GO:0000049", "GO:0000287", "GO:0004826", "GO:0005524", "GO:0006432", "GO:0009328" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQ33", "EC:6.1.1.20", "UniRef:UniRef50_Q9PQ33", "COG:COG0073", "COG:J", "KEGG:K01890", "GO:0000049", "GO:0000287", "GO:0004826", "GO:0005524", "GO:0006432", "GO:0009328" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQ33", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQ33" ], "product": "Phenylalanine--tRNA ligase beta subunit" } }, "id": "NKIECMJPJD_703", "locus": "GCA000828735_00688" }, { "type": "cds", "sequence": "contig_1", "start": 413131, "stop": 413790, "strand": "-", "frame": 3, "gene": "rpe", "product": "D-ribulose-5-phosphate 3 epimerase", "db_xrefs": [ "COG:COG0036", "COG:G", "RefSeq:WP_006688574.1", "SO:0001217", "UniParc:UPI00000C1C75", "UniRef:UniRef100_Q9PQ34", "UniRef:UniRef50_A0AAP9D7K6", "UniRef:UniRef90_Q9PQ34" ], "nt": "ATGAATAAGCAACTAGTTCCATCCCTTTTAGCATTTGATAAAAAATATCCTTATGAACAATTAAAGATCTTTAAAAAGCATCATCTTCAAACAATACATTATGATGTTATGGATCTAACATATGTTAATAACACTGCTTTTGAAACTGAACATTTAGAATGGCTACTAAAAAATGATTTTTTAGTACACATTCATCTAATGGTTATTAACCCCTTAGAAAAAATTAAACAATATTTTATATATAAACCAACTCGCATATCTTTTCATTTTGAAATGAATGATTATCAAACTAATTTATTAATCATTAATGAAATTAAAAAACATCATGTTTTAGCAGGGATTGCAATTCATCCTTGTTTATCACTAAACGATTATGCTTCTTATTTAAAAGAAGTTGATTATATTACTTTTATGTCGGTTATTCCAGGTAAGGGTGGACAAATTTTTTTAGAAGAAAGTTTATTAAAACTAAAAAAATTATATGAATATAAAATTAAAAATAATTTCAAATTTAAGATTGAAATTGATGGTGGTATTACTTTAGAAGTTATTAAAAAAATTAATATTCCAATTGATTTTTTTGTAAGCGGTTCTTATTTAGTGAAAAATATTGAAAAACTAGAGCAAACTGTTAACGAATTTAATCAACTTGTTAATTAA", "aa": "MNKQLVPSLLAFDKKYPYEQLKIFKKHHLQTIHYDVMDLTYVNNTAFETEHLEWLLKNDFLVHIHLMVINPLEKIKQYFIYKPTRISFHFEMNDYQTNLLIINEIKKHHVLAGIAIHPCLSLNDYASYLKEVDYITFMSVIPGKGGQIFLEESLLKLKKLYEYKIKNNFKFKIEIDGGITLEVIKKINIPIDFFVSGSYLVKNIEKLEQTVNEFNQLVN", "aa_hexdigest": "c5ed277c2cd4fa9a73e2cc8924282608", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00000C1C75", "ncbi_nrp_id": "WP_006688574.1", "uniref100_id": "UniRef100_Q9PQ34", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1C75", "RefSeq:WP_006688574.1", "UniRef:UniRef100_Q9PQ34" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQ34", "uniref90_id": "UniRef90_Q9PQ34" }, "psc": { "uniref90_id": "UniRef90_Q9PQ34", "gene": "Rpe", "product": "D-ribulose-5-phosphate 3 epimerase", "uniref50_id": "UniRef50_A0AAP9D7K6", "cog_id": "COG0036", "cog_category": "G" }, "pscc": { "uniref50_id": "UniRef50_A0AAP9D7K6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAP9D7K6" ], "product": "Ribulose-phosphate 3-epimerase" }, "genes": [ "rpe" ], "id": "NKIECMJPJD_704", "locus": "GCA000828735_00689" }, { "type": "cds", "sequence": "contig_1", "start": 413808, "stop": 414458, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTGATAAATTAGTTTACGCTAATAACCCAAGTAAACAATTTAAAGGTGCACTATATGGTTATGGTAGTAGAGAATTATTAATTAAAACATTAGATGGTTTTGTGTCTTATCATGACTACCAAACACTTGCACAATTACTTTATGATCTAAATTTTAATGCAGAAGCTAATGCTGAATTTAAAACTATTTTAGATAACCAAAAACAAATTATTCCTGGTAATCAAAAAGATAGTAAAATAAAAAATATTGATTTAGCAACTAATGAAATTAAAACAATTATGATTAAAGGAATCAATAAAATCCCACAAACTGCCTTTCAAAGATTAGATTTAGATTTAGTTTATAACAAATTAGACAAAAATGCTACACCATTAGTTAATAAAGAAGATAATGCTAATTTAAAATATATTTTATTGGTTTCACAATTTAATGCAAATGATGTGCAAAATTTAGTTATTAACAATCAGTTAAATCAAAGCGCAACAGGATATTTAGGATTGTCAAAAAGCCAATTTTATAACTTATTATTTGATTTAGCATTAAATGATCAAACTTTAAAAATTGAAGCTTTTAGTCAAATTGAAAACGAACATAAAATTAAAGTTTACGATAAACGTTTAGCTAATAATAGTAATTTAAAAGGTTGA", "aa": "MFDKLVYANNPSKQFKGALYGYGSRELLIKTLDGFVSYHDYQTLAQLLYDLNFNAEANAEFKTILDNQKQIIPGNQKDSKIKNIDLATNEIKTIMIKGINKIPQTAFQRLDLDLVYNKLDKNATPLVNKEDNANLKYILLVSQFNANDVQNLVINNQLNQSATGYLGLSKSQFYNLLFDLALNDQTLKIEAFSQIENEHKIKVYDKRLANNSNLKG", "aa_hexdigest": "710ce48c3f9f75190c4449b596ea19b5", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 24618.704700000013, "isoelectric_point": 8.510745429992678 }, "id": "NKIECMJPJD_705", "locus": "GCA000828735_00690" }, { "type": "cds", "sequence": "contig_1", "start": 414972, "stop": 415838, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGTTTGAACGCTCTATTAACAGAGTTAAAAAATGATTTTTTATACAATGGTGCACTATACTTAAATTCACGCAAATCAAATAGTGTTAAAAAACAAGGCTATGATATTAAAGCTAAAATTAAAGATTACTTTAATAACAATAAAGAGCGCTTATTAGCACTATATATTGCTACACATCAAAACGATAGTGATTTTTTGTTTAAATCAAACTTAAAAAATGATTCAAAAGTTTTTGCATTTGATCAAAGAACAATTAATATTATTAAAGAAAACAATGAAATTCATTTATTAAATTACTATGATAATTTAATTGATAAGTTAAATGATAAATTAATTAAATTTAATAAAAAAACATTTATTATTCCTAGTGTTGATAGTTTATATGATAATGGAATTAATACACCAATTTACTTAATTAAAGACCAAAATCAACAAATTAGTTTATTTCAAACTTTAAAAATAAAACTAGTGGGTAATAAAAATCTTATAGATGAAAATCAAAAATTATCTACAATGATTAATGATTATGTTGAAAACTTTGATATAAAAAAACACCTTGTTGCTAATAATAAAAACTCCCAATCAATTGTTTTTGACGATTATGTTATAAATAAAGTATTTAGTAAAGGAATAAACAGTGTTTTTGCAAATATTATTAAACAAGAACAAATTAAAAAAAGTGTAGCAAAATTCTTTGAATTTAATGATTTTAATTTTAAAAATAATTTTCTTGAAAACCAAGAATTATTAGCAGCATTTAACCAAATTATTAAGAATACAAGTGTTTTGGAAACAACACAAACTAATAATTTTAGTAATTTTGATAACTTAAAAAAAGTTTATGAACAAGCTTTGAAATTATGA", "aa": "MGLNALLTELKNDFLYNGALYLNSRKSNSVKKQGYDIKAKIKDYFNNNKERLLALYIATHQNDSDFLFKSNLKNDSKVFAFDQRTINIIKENNEIHLLNYYDNLIDKLNDKLIKFNKKTFIIPSVDSLYDNGINTPIYLIKDQNQQISLFQTLKIKLVGNKNLIDENQKLSTMINDYVENFDIKKHLVANNKNSQSIVFDDYVINKVFSKGINSVFANIIKQEQIKKSVAKFFEFNDFNFKNNFLENQELLAAFNQIIKNTSVLETTQTNNFSNFDNLKKVYEQALKL", "aa_hexdigest": "a23c5b3e2ef44b11fe902e5bcf87db9d", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 33596.88430000002, "isoelectric_point": 9.155300331115722 }, "id": "NKIECMJPJD_706", "locus": "GCA000828735_00691" }, { "type": "cds", "sequence": "contig_1", "start": 416361, "stop": 416618, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTAGAGAATGAAAGTACTTTTAAATCAACTAAAATTGGAGATAGAAACAAAATTGTTGCTAAGACAAATAATTTTTTACAAAAAGGATACGCTAATTTATTTAATTTCTTAATTGATGATTATATTAAATACAACCTGCCTTTATCATTTGCCAACATTGAATTTAAATATGATGATTTAAAAAATCAAAACATTTATAATAAAGATTTTTTTACAAAAATACCAACTTCTGGCACATCACCTAATTTTCCTTGA", "aa": "MLENESTFKSTKIGDRNKIVAKTNNFLQKGYANLFNFLIDDYIKYNLPLSFANIEFKYDDLKNQNIYNKDFFTKIPTSGTSPNFP", "aa_hexdigest": "0b921d1e937cc6a1668754bfd380173c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9929.101899999989, "isoelectric_point": 8.859906196594238 }, "id": "NKIECMJPJD_707", "locus": "GCA000828735_00692" }, { "type": "cds", "sequence": "contig_1", "start": 416925, "stop": 417176, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATTTAAAAAAATTCTTTTAACAAGCACATTAAGTGTTTTAGGGGTTATTTCAATTGCAAGTTTAGCTACAAATTGTTCACAAACGAAAACAAAAATTAACCAGTATAAAATAACTATAAATAAATTAGATAGTCTAAATTTAACAAGTAATTTAACTACAAAACAAGCTTTAAAAGATGTTTTATTCTCAAAAGAAGGACAAAATGAATTTTTATTATCAAATGTTAAAAAAGTGGTTTATGATTGA", "aa": "MKFKKILLTSTLSVLGVISIASLATNCSQTKTKINQYKITINKLDSLNLTSNLTTKQALKDVLFSKEGQNEFLLSNVKKVVYD", "aa_hexdigest": "27b7cee8f791b52476c58fecb614c1c2", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9238.762299999997, "isoelectric_point": 9.76465549468994 }, "id": "NKIECMJPJD_708", "locus": "GCA000828735_00693" }, { "type": "cds", "sequence": "contig_1", "start": 417571, "stop": 417750, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00266.25" ], "nt": "ATGAATGAAACAAGAGCAAATGTTGCCAAATTCATCAATGCTAATTTTGATGAAATCATTTTTACAAGTGGTGCTACAGAATCTCTAAATTTGATTGCAAACGGTTTAAGACCTCATTTAAAAAAGGGTGATGAAATTATTTTAACTTATATTGAACATGCTTCTAATTTATTACCATGA", "aa": "MNETRANVAKFINANFDEIIFTSGATESLNLIANGLRPHLKKGDEIILTYIEHASNLLP", "aa_hexdigest": "8a627341db8848e9d6a827f9c621fb2c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00266.25", "name": "Aminotran_5", "length": 60, "aa_cov": 1.0, "hmm_cov": 1.0, "evalue": 5.685603135050243e-23, "score": 76.99227142333984, "start": 1, "stop": 59 } ], "seq_stats": { "molecular_weight": 6540.372399999998, "isoelectric_point": 5.480040550231934 }, "id": "NKIECMJPJD_709", "locus": "GCA000828735_00694" }, { "type": "cds", "sequence": "contig_1", "start": 417856, "stop": 418596, "strand": "+", "frame": 1, "gene": "csd", "product": "putative cysteine desulfurase", "db_xrefs": [ "COG:COG0520", "COG:E", "EC:2.8.1.7", "GO:0031071", "SO:0001217", "UniRef:UniRef50_Q9PQ36", "UniRef:UniRef90_Q9PQ36" ], "nt": "GTGGTGTCGTTTGCTTCTGGTGGTAATTTAATTGGTAATGTTTTAGATGAAAATCTTATTATTAAAAATATTAAAAAAATTAAACCAGATGTATTAGTGTGCGTTGATGCAACTCAAAGTATTCAACACCGTATGTTTGATGTGAAAAAATGCAAAAGTGATTTTATGGTCTTTTCAGCACATAAATTATTAGGACCTACAGGAATTGGTGTTGCTTATATTAAAAATGACTTGATTAAAAAAATGCAACCATTAAAATATGGTGGTGGAATGAATTTTAGCATTGATTTAAATAGTTATCAGCTCTATGATAATTACATGAAATTTGAAGGTGGAACTCCGCATGTTGCTGGTTTTTATGGTTTTAATGCTGCTTTAAAATTTTTAATGGATATAGGTTATAATAAAATTCATGAACATGAATTAAAGATTACTAAATATGCACGTGACCAATTAGCTTTAATTCCTGAAATTAAAACATATGTGCAAGATATTACTTCAAGTACTATAGCTTTTAATTATAATGGTGTTTTTTGCCAAGATTTTGCTAATTATTTAGGATCAAAAAATATTATTGTGCGAAGTGGATTATCATGTGCAAAAATTATTAATAACGTGATTCAAACCGAATGTGCTATTCGTGCATCTTTTTACATATATAATGATTTTAATGATGTTGATAGATTAATTAAGGCAATTAAAGAATATCAAAAAGGAGATGAATTAAATGGCATCTTATAA", "aa": "MVSFASGGNLIGNVLDENLIIKNIKKIKPDVLVCVDATQSIQHRMFDVKKCKSDFMVFSAHKLLGPTGIGVAYIKNDLIKKMQPLKYGGGMNFSIDLNSYQLYDNYMKFEGGTPHVAGFYGFNAALKFLMDIGYNKIHEHELKITKYARDQLALIPEIKTYVQDITSSTIAFNYNGVFCQDFANYLGSKNIIVRSGLSCAKIINNVIQTECAIRASFYIYNDFNDVDRLIKAIKEYQKGDELNGIL", "aa_hexdigest": "7dd454690cd36c097df22a414e9d4a4d", "start_type": "GTG", "rbs_motif": null, "genes": [ "csd" ], "truncated": "5-prime", "pseudogene": { "start": 417556, "stop": 418596, "observations": { "mutation": [ 417748 ], "stop": [ 417748 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQ36", "query_cov": 1.0, "subject_cov": 0.6119402985074627, "identity": 0.9840000000000001, "score": 489.0, "evalue": 1.41e-172, "gene_start": 1, "gene_end": 246, "reference_start": 157, "reference_end": 402, "reference_sequence": "MNDYKQFFPWFKNNKDVIYLDSSATSLKPQVVIDAIVDYYTKYSTNPHNTDSNFTFHTHEIMNETRANVAKFINANFDEIIFTSGATESLNLIANGLRPHLKKGDEIILTYIEHASNLLPWYKLRDDLGVKIIFANQKNQFPQLSDFLKVISPKTKVVSFASGGNLIGNVLDENLIIKNIKKIKPDVLVCVDATQSIQHRMFDVKKCKSDFMVFSAHKLLGPTGIGVAYIKNDLIKKMQPLKYGGGMNFSIDLNSYQLYDNYMKFEGGTPHVAGFYGFNAALKFLMDIGYNKIHEHELKITKYARDQLALIPEIKTYVQDATSSTITFSYNGVFCQDFANYLGSKNIIVRSGLSCAKIINNVIQTECAIRASFYIYNDFNDVDRLIKAIKEYQKGDELNGIL" }, "paralog": false, "identity": 0.9884393063583815, "score": 686.0, "evalue": 2.4e-252, "description": "Internal stop codon at 417748. Nonsense mutation around 417748.", "psc": { "uniref90_id": "UniRef90_Q9PQ36", "gene": "csd", "product": "Probable cysteine desulfurase", "ec_ids": [ "2.8.1.7" ], "uniref50_id": "UniRef50_Q9PQ36", "cog_id": "COG0520", "cog_category": "E", "go_ids": [ "GO:0031071" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQ36", "EC:2.8.1.7", "UniRef:UniRef50_Q9PQ36", "COG:COG0520", "COG:E", "GO:0031071" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQ36", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQ36" ], "product": "Probable cysteine desulfurase" } }, "id": "NKIECMJPJD_710", "locus": "GCA000828735_00695" }, { "type": "cds", "sequence": "contig_1", "start": 418583, "stop": 419050, "strand": "+", "frame": 2, "gene": "iscU", "product": "Iron-sulfur cluster assembly scaffold protein", "db_xrefs": [ "COG:COG0822", "COG:O", "RefSeq:WP_045470481.1", "SO:0001217", "UniParc:UPI0004563D34", "UniRef:UniRef100_UPI0004563D34", "UniRef:UniRef50_A0A084F069", "UniRef:UniRef90_A0AAC9T0G2" ], "nt": "ATGGCATCTTATAATATCAATGACAATCTTTTATTACGTAAGATTATCATGCAACACTATGAACACCCTAAAAATAAAGTTTGTTTTGTACAAGATGAAATAAATTATTTGTCATATCATAATACAACAGAAGGATGTGGAGATGACATTACAGTTTATGTTAAATTAAATCATCAAAAAATAATTGATGTCGTTTTTTTAGGTACAGGCTGTGCAATTTCCATATCATCCACTGATATTATTTGTGAACTTGTAAAAAATCTAGATCTTAATAATGCGTTATTATTAATTAATAATTATTTAAATATGATTGAAGGGCTAGATTACAATAAAGATATTATGCAAGAATTAATTGCTTTTTATAATGTAAAAAAACAAATGAATCGTATTCGTTGTGCCCGTATTGGCGCAAGTGCTTTAAAAATGTGTTTAAAGCAATATACAGATAAACACAAAACAATTGTTTAA", "aa": "MASYNINDNLLLRKIIMQHYEHPKNKVCFVQDEINYLSYHNTTEGCGDDITVYVKLNHQKIIDVVFLGTGCAISISSTDIICELVKNLDLNNALLLINNYLNMIEGLDYNKDIMQELIAFYNVKKQMNRIRCARIGASALKMCLKQYTDKHKTIV", "aa_hexdigest": "d0e182162fc322bfb6a5dd46c00e41b2", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0004563D34", "ncbi_nrp_id": "WP_045470481.1", "uniref100_id": "UniRef100_UPI0004563D34", "db_xrefs": [ "SO:0001217", "UniParc:UPI0004563D34", "RefSeq:WP_045470481.1", "UniRef:UniRef100_UPI0004563D34" ] }, "ips": { "uniref100_id": "UniRef100_UPI0004563D34", "uniref90_id": "UniRef90_A0AAC9T0G2" }, "psc": { "uniref90_id": "UniRef90_A0AAC9T0G2", "gene": "IscU", "product": "Iron-sulfur cluster assembly scaffold protein", "uniref50_id": "UniRef50_A0A084F069", "cog_id": "COG0822", "cog_category": "O" }, "pscc": { "uniref50_id": "UniRef50_A0A084F069", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A084F069" ], "product": "Nitrogen fixation protein NifU" }, "genes": [ "iscU" ], "id": "NKIECMJPJD_711", "locus": "GCA000828735_00696" }, { "type": "cds", "sequence": "contig_1", "start": 419280, "stop": 419552, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00587.30" ], "nt": "ATGCCAACTTTAATTCCTTTAAGTGAATTTCAAAAAGAAAAAGACCATATTGAAGGTTTTGCACCTGAATTATTTATGGTTAATCAAATTGGTGATAAAAAATTAGATAATTCTTATGCAATACGTCCTACGAGTGAAATTTTATTTTGTAATTATTTTAAAAATATTGTTAATAGTTATAATGATTTACCGATTAAGAATAATCAATGGTGTTCAGTAATGCGTGCTGAAAAAACAACACGTCCATTTCTTCGAAATGCTGAGTTTCATTGA", "aa": "MPTLIPLSEFQKEKDHIEGFAPELFMVNQIGDKKLDNSYAIRPTSEILFCNYFKNIVNSYNDLPIKNNQWCSVMRAEKTTRPFLRNAEFH", "aa_hexdigest": "cdbb67b52f171035ed67227a914633a6", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00587.30", "name": "tRNA-synt_2b", "length": 59, "aa_cov": 0.6333333333333333, "hmm_cov": 0.9830508474576272, "evalue": 6.526475818180162e-11, "score": 38.15842056274414, "start": 34, "stop": 90 } ], "seq_stats": { "molecular_weight": 10598.02819999999, "isoelectric_point": 6.715720176696778 }, "id": "NKIECMJPJD_712", "locus": "GCA000828735_00697" }, { "type": "cds", "sequence": "contig_1", "start": 419727, "stop": 420494, "strand": "+", "frame": 3, "gene": "proS", "product": "proline--tRNA ligase", "db_xrefs": [ "COG:COG0442", "COG:J", "EC:6.1.1.15", "GO:0004827", "GO:0005524", "GO:0005737", "GO:0006433", "GO:0017101", "SO:0001217", "UniRef:UniRef50_Q9PQ38", "UniRef:UniRef90_Q9PQ38" ], "nt": "ATGATGCAAGATGGCCAAGCATTACAATCTGCAACAAGTCATTATTTAGGGCAGTTTTTTGCAAAAGCTTATGATATTAAGTTTCAAGGTCAAGATAATCAAATGCATTATGTACATCAAATGTCTGCTGGATTATCAACACGAATTATTGGAGCATTAATTATGGTTCATGCTGATGATCAGGGTTTGATTTTGCCCCCTGATATTGCTTTTAATCAAATTGCTATTTTAACGATTTTTTCTAATAAAAATCCCCAATTATTAAAAATTAGTCAACAGATTTGTGAACAATTAAATGGTTATCGTTTGTTTGAAGATCACAGTGATAAAGGAATTGGTTATAAATTAGCACAACAAGAAATTGAGGGAACGCCTATTTGTATATTAGTTGGTGCTAAAGAATTAGTTAATCAACAAGTTGTTATTGTACGTCGTGATACTCATGAAAAAATTAATGTTGATTTAACTGATTTGAAACTAATTATCCCAAAATTATTAGCAGATATTAAAAGAAATATTTATGAAAAAGCAAAAAAAGATTTAGAGGATTCGATTGTTTTTGTGAATAATATCGAGGAATTAAAACAAGTTATTGCACAAAATAAAATGGCTAAGGCATTCTTTGATGGTACAAAAGAAGATGATGAACAAATTAAATTATTAACTAATGCTTCAACACGTTGTATTTTTGATGAAACTCAAAACGGGCAGTGTTTTTATACAAATAAAAAAACAAACAAATTGACTTTATTTGCACGTGCTTATTAG", "aa": "MMQDGQALQSATSHYLGQFFAKAYDIKFQGQDNQMHYVHQMSAGLSTRIIGALIMVHADDQGLILPPDIAFNQIAILTIFSNKNPQLLKISQQICEQLNGYRLFEDHSDKGIGYKLAQQEIEGTPICILVGAKELVNQQVVIVRRDTHEKINVDLTDLKLIIPKLLADIKRNIYEKAKKDLEDSIVFVNNIEELKQVIAQNKMAKAFFDGTKEDDEQIKLLTNASTRCIFDETQNGQCFYTNKKTNKLTLFARAY", "aa_hexdigest": "6be2b23535b2afe708a4c57e1f9450bd", "start_type": "ATG", "rbs_motif": null, "genes": [ "proS" ], "truncated": "5-prime", "pseudogene": { "start": 419427, "stop": 420494, "observations": { "mutation": [ 419550, 419682 ], "stop": [ 419550, 419682 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQ38", "query_cov": 1.0, "subject_cov": 0.5402542372881356, "identity": 0.996, "score": 500.0, "evalue": 1.29e-175, "gene_start": 1, "gene_end": 255, "reference_start": 218, "reference_end": 472, "reference_sequence": "MSKKLEKITTRKENFADWYTSIVNNAKLIQYTDIKGMMVFQPNAWAIWEAIKNQIDVEFKKHGVRNLAMPTLIPLSEFQKEKDHIEGFAPELFMVNQIGDKKLDNSYAIRPTSEILFCNYFKNIVNSYNDLPIKNNQWCSVMRAEKTTRPFLRNAEFHWQELHAIFASENEADSFAKVILDVYTDFVQNYLCIPVIKGLKTPWERFAGAQKTYTIEAMMQDGQALQSATSHYLGQFFAKAYDIKFQGQDNQMHYVHQMSAGLSTRIIGALIMVHADDQGLILPPDIAFNQIAILTIFSNKNPQLLKISQQICEQLNNYRLFEDHSDKGIGYKLAQQEIEGTPICILVGAKELVNQQVVIVRRDTHEKINVDLTDLKLIIPKLLADIKRNIYEKAKKDLEDSIVFVNNIEELKQVIAQNKMAKAFFDGTKEDDEQIKLLTNASTRCIFDETQNGQCFYTNKKTNKLTLFARAY" }, "paralog": false, "identity": 0.9915492957746479, "score": 702.0, "evalue": 1.84e-257, "description": "Internal stop codon at 419550, 419682. Nonsense mutation around 419550, 419682.", "psc": { "uniref90_id": "UniRef90_Q9PQ38", "gene": "proS", "product": "proline--tRNA ligase", "ec_ids": [ "6.1.1.15" ], "uniref50_id": "UniRef50_Q9PQ38", "cog_id": "COG0442", "cog_category": "J", "go_ids": [ "GO:0004827", "GO:0005524", "GO:0005737", "GO:0006433", "GO:0017101" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQ38", "EC:6.1.1.15", "UniRef:UniRef50_Q9PQ38", "COG:COG0442", "COG:J", "GO:0004827", "GO:0005524", "GO:0005737", "GO:0006433", "GO:0017101" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQ38", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQ38" ], "product": "Proline--tRNA ligase" } }, "id": "NKIECMJPJD_713", "locus": "GCA000828735_00698" }, { "type": "cds", "sequence": "contig_1", "start": 420592, "stop": 420762, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGATTATTTAGTAAGAAAAATAAATTAAATCAAAAGTTAACTGAAGATAAAAATATAAATATTACTGATGTTTTAGATAATAACAAAGATATTTATCAATTATATGATGAAACTATTGTTACTAAAAAAGTTAAAGTAAGATGCAAAAATTGTGGCGTTTTTATGTGA", "aa": "MGLFSKKNKLNQKLTEDKNINITDVLDNNKDIYQLYDETIVTKKVKVRCKNCGVFM", "aa_hexdigest": "f6db3a32dd4346950941d6a8792743d3", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6553.606300000001, "isoelectric_point": 9.230728340148925 }, "id": "NKIECMJPJD_714", "locus": "GCA000828735_00699" }, { "type": "cds", "sequence": "contig_1", "start": 420838, "stop": 421239, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATGAACCACAAATTTTACAAGATTGCAATGATCTATCATTAGCTCCAGTTAATAACCAAGAAGATGATATTGTTAACGAACATGAATCTACACCTATTGAACCATTACAAGAAGAAGTTGAACCTGAGCCTATAAAGAACGAAAATCAAAGCAATGAAGAATTTATACAAGAGAATATTGTAGCTCCAGTTGATGAAGTTGATAATTTAGTAAAAGAACATACACCTTTTACTCCTGTTTCTTTTGAATTTGAAGAACCTGTTTTTGAAAATATTTATTCAAACCAAAAAGTGTATGCTGAAAATTTATTTTTAGAATCTTTATTTTCTGAAAAAGAAAGTTTAATTAATGATGATCAAAATGATTTTAGTTGGTTATCAAAGTATGTGAGTGTTTGA", "aa": "MDEPQILQDCNDLSLAPVNNQEDDIVNEHESTPIEPLQEEVEPEPIKNENQSNEEFIQENIVAPVDEVDNLVKEHTPFTPVSFEFEEPVFENIYSNQKVYAENLFLESLFSEKESLINDDQNDFSWLSKYVSV", "aa_hexdigest": "ff2c598b552dcf9bf0ef3af8f9f7825e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15435.53669999999, "isoelectric_point": 4.0500284194946286 }, "id": "NKIECMJPJD_715", "locus": "GCA000828735_00700" }, { "type": "cds", "sequence": "contig_1", "start": 422136, "stop": 422342, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGGCCAAGTTATAGTAACTATACCATCTTTTATTTATATTAATTATGAATTAATAACTTATGCAGTTAATAATAAAACAAGTTTACCCTTTTATTATTATTTGGCTGTTGTTGGTATAATTGTTGATTTATGTATGACAGCTATTTATTTTGTTTTAGGCTTCTCTAAAAAATTGCATTTTCGTTTAGTTTTAATTTATAATTGA", "aa": "MGQVIVTIPSFIYINYELITYAVNNKTSLPFYYYLAVVGIIVDLCMTAIYFVLGFSKKLHFRLVLIYN", "aa_hexdigest": "f51044952080a3552f4651a75160fd7b", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7892.380399999999, "isoelectric_point": 8.788281822204592 }, "id": "NKIECMJPJD_716", "locus": "GCA000828735_00701" }, { "type": "cds", "sequence": "contig_1", "start": 422430, "stop": 422699, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTATTATTTTAAAACACTGCGGGTTTCATTATTGGCTTTTGTTATTGGAGTAGTCTATAACATTGGTATTTATGCACTAATGTATATTTCATATCGTTTAGTAGAACCAAAACCACATAACATCTTTTTTGATTTATTTAATTATACAAATATTGCTGTCACAGCAACTAATTTTGTCCCTTTGCCTGGATCTGAGGGTTCAATTCAATTTATGTTACGTGTTTTTTTAACAAATGATCAAAATCAAATTCGTTACGCAATGAATTGA", "aa": "MYYFKTLRVSLLAFVIGVVYNIGIYALMYISYRLVEPKPHNIFFDLFNYTNIAVTATNFVPLPGSEGSIQFMLRVFLTNDQNQIRYAMN", "aa_hexdigest": "c2125aedb287350c025bb165cbf9437b", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10372.024599999995, "isoelectric_point": 9.048347282409669 }, "id": "NKIECMJPJD_717", "locus": "GCA000828735_00702" }, { "type": "cds", "sequence": "contig_1", "start": 422742, "stop": 422852, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTAGTATTTATTTAGGTAGTTTAATTGGGATGTTTGGGTTTGGTTATTTTTTAATTTTTGATTTATATAAATTTATTAAAAAAGCTAAAGTTGATAAAAAAAGATAA", "aa": "MFSIYLGSLIGMFGFGYFLIFDLYKFIKKAKVDKKR", "aa_hexdigest": "c51d02e559d8b40f1a87c787efd16cb0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4286.193500000002, "isoelectric_point": 9.901779747009275 }, "id": "NKIECMJPJD_718", "locus": "GCA000828735_00703" }, { "type": "cds", "sequence": "contig_1", "start": 422894, "stop": 423322, "strand": "+", "frame": 2, "gene": "ruvA", "product": "Holliday junction branch migration protein RuvA", "db_xrefs": [ "COG:COG0632", "COG:L", "EC:3.6.4.12", "GO:0000400", "GO:0003678", "GO:0005524", "GO:0005737", "GO:0006281", "GO:0006310", "GO:0009378", "GO:0009379", "GO:0036121", "GO:0048476", "GO:0061749", "GO:1990518", "SO:0001217", "UniRef:UniRef50_Q9PQ41", "UniRef:UniRef90_Q9PQ41" ], "nt": "ATGAACTATTTAATTTTTAAAGTTATTTATGCAAATGCAAATATTGTTGTTGGTGAACATAATTTTATTGGTTATCAAATACGTATCCCAAAAGATTATGAATTAGAAATTAATAAATTTTGCAAATTATATTTGTATGAATATATTAGCATAATGCCTAATAAAAATTTAATTATTAAAGATTTATATGGTTTTCGTACTTATAATGAACGGTTATTATTTATAGACTTGATTAGTACTAATTCAATTGGCCCAAAAACAGCTATTAATATTCTAAAATATGATATTGATACAATTATTGATGCAATTGCTACTAAGAATATAGATTATTTAATAACAATTAAAGGAATTAACCAACGAAATGCTAATTTGATTTGTGATCAATTAAATTATAAATACATAAATAAAGTAAATGATAAAAATAACTGA", "aa": "MNYLIFKVIYANANIVVGEHNFIGYQIRIPKDYELEINKFCKLYLYEYISIMPNKNLIIKDLYGFRTYNERLLFIDLISTNSIGPKTAINILKYDIDTIIDAIATKNIDYLITIKGINQRNANLICDQLNYKYINKVNDKNN", "aa_hexdigest": "fd2ea976cf7927ece6ecb674431013a0", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "genes": [ "ruvA" ], "truncated": "3-prime", "pseudogene": { "start": 422894, "stop": 423482, "observations": { "insertion": [ 423338 ], "mutation": [ 423320 ], "stop": [ 423320 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQ41", "query_cov": 1.0, "subject_cov": 0.7282051282051282, "identity": 0.993, "score": 280.0, "evalue": 1.43e-94, "gene_start": 1, "gene_end": 142, "reference_start": 1, "reference_end": 142, "reference_sequence": "MNYLIFKVIYANANIVVGEHNFIGYQIRIPKDYELEINKFCKLYLYEYISIMPNKNLIIKDLYGFRTYNERLLFIDLISINSIGPKTAINILKYDIDTIIDAIATKNIDYLITIKGINQRNANLICDQLNYKYINKVNDKNNWAKELSIGLENLGYTKKDIEYAITKVKINSQQDIDISEIISSAIKEISLRHEN" }, "paralog": false, "identity": 0.9897435897435898, "score": 376.0, "evalue": 5.34e-135, "description": "Internal stop codon at 423320. Frameshift due to insertion around 423338. Nonsense mutation around 423320.", "psc": { "uniref90_id": "UniRef90_Q9PQ41", "gene": "ruvA", "product": "Holliday junction branch migration protein RuvA", "ec_ids": [ "3.6.4.12" ], "uniref50_id": "UniRef50_Q9PQ41", "cog_id": "COG0632", "cog_category": "L", "go_ids": [ "GO:0000400", "GO:0003678", "GO:0005524", "GO:0005737", "GO:0006281", "GO:0006310", "GO:0009378", "GO:0009379", "GO:0036121", "GO:0048476", "GO:0061749", "GO:1990518" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQ41", "EC:3.6.4.12", "UniRef:UniRef50_Q9PQ41", "COG:COG0632", "COG:L", "GO:0000400", "GO:0003678", "GO:0005524", "GO:0005737", "GO:0006281", "GO:0006310", "GO:0009378", "GO:0009379", "GO:0036121", "GO:0048476", "GO:0061749", "GO:1990518" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQ41", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQ41" ], "product": "Holliday junction branch migration complex subunit RuvA" } }, "id": "NKIECMJPJD_719", "locus": "GCA000828735_00704" }, { "type": "cds", "sequence": "contig_1", "start": 423657, "stop": 424385, "strand": "+", "frame": 3, "gene": "ruvB", "product": "Holliday junction branch migration DNA helicase RuvB", "db_xrefs": [ "COG:COG2255", "COG:L", "EC:3.6.4.-", "EC:3.6.4.12", "GO:0000400", "GO:0003677", "GO:0003689", "GO:0005524", "GO:0005737", "GO:0006281", "GO:0006310", "GO:0009378", "GO:0016887", "GO:0036121", "GO:0048476", "GO:0061749", "GO:0061775", "GO:0140584", "GO:0140665", "GO:0140849", "GO:1990518", "SO:0001217", "UniRef:UniRef50_Q9PQ42", "UniRef:UniRef90_Q9PQ42" ], "nt": "ATGAATGTTAATTGTCACATAACACAAGGGAACTTGTTAAATAAACCTATTGATATTATTAATTTATTATCTTTAATTAAAGAAAATGACGTTGTTTTTATTGATGAAATTCACGCTTGTGGTTTAGGTGCATTTGAAACACTTTATTCTGTATTAGAAGATTTTTGTATTGATATTAATATAGGTAAAGATTTTAATTCAAAAATGACGAGGTTAAAGATTCCTCATTTTACACTTATTGGTGCAACAACAATTTTTGGAAAAATCCCAAAACCTTTAGAAGAACGCTTCGGACACATCTTTCATTTAAATGAATATGAACCATCAGAAATTAGTGCAATTATTTTAAAGAATAATCAGATGCATTTTCAGATTGATTTAAACGAAGAAGAAATTGATTTGATTGCTAATAATGCTAAAGGAATCCCACGATTAGCAAATCGATTATTAAAACGAGTTGTAGATTTTAAAATTAATGGGTTTAATGATATTAAAAATATTTTTAAAAAAATTCAAATTTATGAGTTTGGATTAGATGAACAAGATATTAATTATCTAAATGTTTTATATCGACAAGATAATGAAATTGGTTTAAAATCAATTGCACAGATTTTACGTTTAGATCAATATACAATTGAAACAAAAATAGAACCTTATTTAATTCAACATCATTTTATAAATAAGAATTTAAGAGGTCGAAAAATTACAATTGAAGGAATAGATTTTTAA", "aa": "MNVNCHITQGNLLNKPIDIINLLSLIKENDVVFIDEIHACGLGAFETLYSVLEDFCIDINIGKDFNSKMTRLKIPHFTLIGATTIFGKIPKPLEERFGHIFHLNEYEPSEISAIILKNNQMHFQIDLNEEEIDLIANNAKGIPRLANRLLKRVVDFKINGFNDIKNIFKKIQIYEFGLDEQDINYLNVLYRQDNEIGLKSIAQILRLDQYTIETKIEPYLIQHHFINKNLRGRKITIEGIDF", "aa_hexdigest": "71c4674ca1d72fba844688f9216ea4cb", "start_type": "ATG", "rbs_motif": null, "genes": [ "ruvB" ], "truncated": "both", "pseudogene": { "start": 423472, "stop": 424408, "observations": { "deletion": [ 423505, 424381 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQ42", "query_cov": 1.0, "subject_cov": 0.7756410256410257, "identity": 0.996, "score": 483.0, "evalue": 2.66e-171, "gene_start": 1, "gene_end": 242, "reference_start": 63, "reference_end": 304, "reference_sequence": "MKTNYEFRPQYLKDFIGKEQLKNNLKVYLTASKRLENSFDHTLLHGLSGTGKTTLALIIANEMNVNCHITQGNLLNKPIDIINLLSLIKENDVVFIDEIHACGLGAFETLYSVLEDFCIDINIGKDFNSKMTRLKIPHFTLIGATTIFGKIPKSLEERFGHIFHLNEYEPSEISAIILKNNQMHFQIDLNEEEIDLIANNAKGIPRLANRLLKRVVDFKINGFNDIKNIFKKIQIYEFGLDEQDINYLNVLYRQDNEIGLKSIAQILRLDQYTIETKIEPYLIQHHFINKNLRGRKITIEGIDFLKNNQLIK" }, "paralog": false, "identity": 0.9935897435897436, "score": 607.0, "evalue": 5.82e-223, "description": "Frameshift due to deletion around 423505, 424381.", "psc": { "uniref90_id": "UniRef90_Q9PQ42", "gene": "ruvB", "product": "Holliday junction branch migration DNA helicase RuvB", "ec_ids": [ "3.6.4.-", "3.6.4.12" ], "uniref50_id": "UniRef50_Q9PQ42", "cog_id": "COG2255", "cog_category": "L", "go_ids": [ "GO:0000400", "GO:0003677", "GO:0003689", "GO:0005524", "GO:0005737", "GO:0006281", "GO:0006310", "GO:0009378", "GO:0016887", "GO:0036121", "GO:0048476", "GO:0061749", "GO:0061775", "GO:0140584", "GO:0140665", "GO:0140849", "GO:1990518" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQ42", "EC:3.6.4.-", "EC:3.6.4.12", "UniRef:UniRef50_Q9PQ42", "COG:COG2255", "COG:L", "GO:0000400", "GO:0003677", "GO:0003689", "GO:0005524", "GO:0005737", "GO:0006281", "GO:0006310", "GO:0009378", "GO:0016887", "GO:0036121", "GO:0048476", "GO:0061749", "GO:0061775", "GO:0140584", "GO:0140665", "GO:0140849", "GO:1990518" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQ42", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQ42" ], "product": "Holliday junction branch migration complex subunit RuvB" } }, "id": "NKIECMJPJD_720", "locus": "GCA000828735_00705" }, { "type": "cds", "sequence": "contig_1", "start": 424397, "stop": 425479, "strand": "-", "frame": 2, "gene": "dinB", "product": "DNA polymerase IV", "db_xrefs": [ "COG:COG0389", "COG:L", "EC:2.7.7.7", "GO:0000287", "GO:0003684", "GO:0003887", "GO:0005829", "GO:0006261", "GO:0006281", "GO:0009432", "GO:0042276", "RefSeq:WP_006689302.1", "SO:0001217", "UniParc:UPI00016C04D8", "UniRef:UniRef100_UPI00016C04D8", "UniRef:UniRef50_Q9PQ53", "UniRef:UniRef90_Q9PQ53" ], "nt": "ATGAAAAATAATCATCAAAAAATCATCATGCATCTTGATATCGATGCTTTTTATGCTACTGTTAGTGAACTATTACATCCTGAATATAAAAATTTTCCAATTGCTGTTGGTTCATTAAATTCACGCACAGGTATTATTTCTTCACCTAACTATTTAGCACGTTCTTATGGCGTTAAAGCTGCAATGCCTATCTTTTTAGCTAAAGAATTATGTCCAAATTTAATTATTTTACCTTCTGAACACAATATTTATCAATCTTATTCAAAACATTTTTTTCAAATCATTAATAAATATGCAAATAAGATTGAAATAACTTCAATTGATGAATGCTTTATTGATGCAACAGATTTAGTTAAAAAATATCATAATAATATCCGCTTGTTAGCAACAAAAATACAAAAAGAAATCAAAAACAAACTAAACCTTAGTATTTCGATTGGAATTTCATATAATAAAACAATTGCAAAAATGGCAACGGAATTAAATAAACCTTCTGGTATATCAATTATTGATGAAAATAAAATTATTAATTTAATTTATGAATTAAATATCAATAAAATTCCCTATATTGGCGAAATAAAATCACAAGAATTATATGCGATTAACATTTTTAAAATTAAAGAATTAATTGCAACTGAAAATAAACAAAAAATTTCATTAGTTTTAGGTTCAATATATCAAAATTTAGTCAATGATTTAAAAGGTTTAAGTGAAATTAAAATAATTGATGAAGATATTTATAAAACAATTTCACATTCGAAAACATTTAACGAAGATTTAAATGATTTTTATGAAATTTCTAATGAAATGAACGATTTAATTTTAACTGTTACTAATCGATTAAAAAAATGTAATTTAATGACTAATAACATCAGTATTTATATTAAATATCCTAGTTTTAAAACAAAAATTAAGCAAAAACAATTAACTTATTACACTGATGATTATCAAACCATTTTTCTAGCAATTAAAAACTTATTTAAAATAATGTATAAAGATGAAACAATACGTTTAATTGGTATCTCACTAAATAAATTAGTAAAAAAAGAAAATGTTAAAAAACAATTATTTTTATTTGATTAA", "aa": "MKNNHQKIIMHLDIDAFYATVSELLHPEYKNFPIAVGSLNSRTGIISSPNYLARSYGVKAAMPIFLAKELCPNLIILPSEHNIYQSYSKHFFQIINKYANKIEITSIDECFIDATDLVKKYHNNIRLLATKIQKEIKNKLNLSISIGISYNKTIAKMATELNKPSGISIIDENKIINLIYELNINKIPYIGEIKSQELYAINIFKIKELIATENKQKISLVLGSIYQNLVNDLKGLSEIKIIDEDIYKTISHSKTFNEDLNDFYEISNEMNDLILTVTNRLKKCNLMTNNISIYIKYPSFKTKIKQKQLTYYTDDYQTIFLAIKNLFKIMYKDETIRLIGISLNKLVKKENVKKQLFLFD", "aa_hexdigest": "41dea16b4c1fba979286e89bcef6c8b6", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00016C04D8", "ncbi_nrp_id": "WP_006689302.1", "uniref100_id": "UniRef100_UPI00016C04D8", "db_xrefs": [ "SO:0001217", "UniParc:UPI00016C04D8", "RefSeq:WP_006689302.1", "UniRef:UniRef100_UPI00016C04D8" ] }, "ips": { "uniref100_id": "UniRef100_UPI00016C04D8", "uniref90_id": "UniRef90_Q9PQ53" }, "psc": { "uniref90_id": "UniRef90_Q9PQ53", "gene": "dinB", "product": "DNA polymerase IV", "ec_ids": [ "2.7.7.7" ], "uniref50_id": "UniRef50_Q9PQ53", "cog_id": "COG0389", "cog_category": "L", "go_ids": [ "GO:0000287", "GO:0003684", "GO:0003887", "GO:0005829", "GO:0006261", "GO:0006281", "GO:0009432", "GO:0042276" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQ53", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQ53" ], "product": "DNA polymerase IV" }, "genes": [ "dinB" ], "id": "NKIECMJPJD_721", "locus": "GCA000828735_00706" }, { "type": "cds", "sequence": "contig_1", "start": 425469, "stop": 426212, "strand": "-", "frame": 1, "gene": "yqxC", "product": "Hemolysin", "db_xrefs": [ "COG:COG1189", "COG:J", "EC:2.1.1.226", "EC:2.1.1.227", "KEGG:K06442", "RefSeq:WP_006689051.1", "SO:0001217", "UniParc:UPI00000C1C68", "UniRef:UniRef100_Q9PQ54", "UniRef:UniRef50_Q9PQ54", "UniRef:UniRef90_Q9PQ54" ], "nt": "ATGAATCAAAGGTTAGATCATTATCTGAAAAACACTAATCAATGTGAAACACGATCCAAAGCAATTGATTTGATAAAACGCGGACGTGTGTTTGTTAATAATTTTCAAGTATTAAAACCCTCATTTTTAGTTAAACAGACTGATTCTGTAGAAATTAAAAATGATGAAATGTATTTCGTTTCTAAAGGTGGATATAAGCTTTTAAAAATTATTAAAGAAATTAATTTAATAATTAAAAATTTTGTTATAGCTGATTTAGGGAGCTCAACAGGAGGTTTTACTGATTGTTGTTTACAATTGGGTGCTAAAAAAATTTACGCAATCGATGTTGGTGTTGACTTATTACACCCTAGTTTAAAGAAAAGTTATAAAATTATTAATTATGAAAAAACTAATGTTAAAAATTTAGATAAATCTTATTTTTTAGAAGATTTAGATTTAATTGTTGGTGATTTATCTTTCATTTCTTTAGAGCAAATTTTTCCAATAATTAAAAAAATTTCATCCCCTAAAACTTTGCTTTTATTGTTGATAAAACCCCAATTTGAATTAGGAAAGGACGTAGCTAGTAAATATAAAGGTCTAATTCAAAATCAAAAATTACAACAATTAGCAATAAACAAAATAATCAATTTAGCAAAAAATTATGACTTTGAATGTAAACATTTAACTCCTACTGATATTTTTGATGAAAAAAAACAAAATCAAGAATATATGATATTTTTACAAAAGCATGAAAAATAA", "aa": "MNQRLDHYLKNTNQCETRSKAIDLIKRGRVFVNNFQVLKPSFLVKQTDSVEIKNDEMYFVSKGGYKLLKIIKEINLIIKNFVIADLGSSTGGFTDCCLQLGAKKIYAIDVGVDLLHPSLKKSYKIINYEKTNVKNLDKSYFLEDLDLIVGDLSFISLEQIFPIIKKISSPKTLLLLLIKPQFELGKDVASKYKGLIQNQKLQQLAINKIINLAKNYDFECKHLTPTDIFDEKKQNQEYMIFLQKHEK", "aa_hexdigest": "13d1350d7cb3675727249f79e4292af5", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI00000C1C68", "ncbi_nrp_id": "WP_006689051.1", "uniref100_id": "UniRef100_Q9PQ54", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1C68", "RefSeq:WP_006689051.1", "UniRef:UniRef100_Q9PQ54" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQ54", "uniref90_id": "UniRef90_Q9PQ54" }, "psc": { "uniref90_id": "UniRef90_Q9PQ54", "gene": "YqxC", "product": "Hemolysin", "ec_ids": [ "2.1.1.226", "2.1.1.227" ], "uniref50_id": "UniRef50_Q9PQ54", "cog_id": "COG1189", "cog_category": "J", "kegg_orthology_id": "K06442" }, "pscc": { "uniref50_id": "UniRef50_Q9PQ54", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQ54" ], "product": "Hemolysin" }, "genes": [ "yqxC" ], "id": "NKIECMJPJD_722", "locus": "GCA000828735_00707" }, { "type": "cds", "sequence": "contig_1", "start": 426295, "stop": 426906, "strand": "-", "frame": 3, "gene": "fepC", "product": "Ferrichrome transport ATP-binding protein", "db_xrefs": [ "COG:COG1120", "COG:PH", "SO:0001217", "UniRef:UniRef50_A0A084EXZ1", "UniRef:UniRef90_Q9PQ55" ], "nt": "ATGGGTCGTTTTCCAAGTTCAAATGCTTTGGGAATTAATACAAATACAGAACGTGAAAAACAAATTATTGATGAAGCTTTGAAAAATGTTGGTATTTATGAATTCCGCCACAAAAATCTTGAAGATTTATCAGGTGGTCAAAAACAAAAAGCATTAATTGCTTTAGCACTTGCTCAAGATACTGAAACAATTGTTTTAGATGAACCTACTAACCACTTAGATATAAGAAGTCAATTAGAAATTATTGAACTTTTACATAAATTACACCACGAAATGAAAAAAACAATTGTTTTAGTTATTCATGATATTAATAATGGTTTAAAATATGCACATAAAGTCATGATTATGAAAAATGGTGAAATGGTTCGTTATGGAAAATTAAAAGAAACAATCGATCATGAAATTTTATTAGATGTTTTTGGTGTTGAATCAATTATTGTGAAAAATGAATCGACATATCCACAAGTTTCTGTAACAGATTTTAGTTTACCAAAAGACTATAAAATTAATGAAATCAAAGAAAACGAAAAAACTAATTCAATTTATAATGATGGTGATGAAGTAGAAATTGAAAATGAATTAGAAAAAGAAGTTGATAAAACAAAAAAATAA", "aa": "MGRFPSSNALGINTNTEREKQIIDEALKNVGIYEFRHKNLEDLSGGQKQKALIALALAQDTETIVLDEPTNHLDIRSQLEIIELLHKLHHEMKKTIVLVIHDINNGLKYAHKVMIMKNGEMVRYGKLKETIDHEILLDVFGVESIIVKNESTYPQVSVTDFSLPKDYKINEIKENEKTNSIYNDGDEVEIENELEKEVDKTKK", "aa_hexdigest": "b29b32418c5f0c8efa145c4c70ca7496", "start_type": "ATG", "rbs_motif": null, "genes": [ "fepC" ], "truncated": "3-prime", "pseudogene": { "start": 426295, "stop": 427206, "observations": { "mutation": [ 427029, 427089, 427104 ], "stop": [ 427029, 427089, 427104 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQ55", "query_cov": 1.0, "subject_cov": 0.5218508997429306, "identity": 1.0, "score": 391.0, "evalue": 9.53e-135, "gene_start": 1, "gene_end": 203, "reference_start": 185, "reference_end": 387, "reference_sequence": "MSFFKKKNDLQKEEHKHVSVVDMNEILSYEDSEEFKNDEIDSNEPIFKLNNVSLGYGKKMIIKNLTATIHRNDFVVVLGPNGSGKSTLIKGLCRINNPSSGYIMYRDKLISRPLLPFLWLEKAWSSIVNIFTKDRKEVIETIDWHIQNYKKIHAYKSKELALNLAYVPQLAVFPEATSIYDFVKMGRFPSSNALGINTNTEREKQIIDEALKNVGIYEFRHKNLEDLSGGQKQKALIALALAQDTETIVLDEPTNHLDIRSQLEIIELLHKLHHEMKKTIVLVIHDINNGLKYAHKVMIMKNGEMVRYGKLKETIDHEILLDVFGVESIIVKNESTYPQVSVTDFSLPKDYKINEIKENEKTNSIYNDGDEVEIENELEKEVDKTKKIN" }, "paralog": false, "identity": 0.9900990099009901, "score": 586.0, "evalue": 7.39e-214, "description": "Internal stop codon at 427029, 427089, 427104. Nonsense mutation around 427029, 427089, 427104.", "psc": { "uniref90_id": "UniRef90_Q9PQ55", "gene": "FepC", "product": "Ferrichrome transport ATP-binding protein", "uniref50_id": "UniRef50_A0A084EXZ1", "cog_id": "COG1120", "cog_category": "PH", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQ55", "UniRef:UniRef50_A0A084EXZ1", "COG:COG1120", "COG:PH" ] }, "pscc": { "uniref50_id": "UniRef50_A0A084EXZ1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A084EXZ1" ], "product": "ABC transporter ATP-binding protein" } }, "id": "NKIECMJPJD_723", "locus": "GCA000828735_00708" }, { "type": "cds", "sequence": "contig_1", "start": 427102, "stop": 427458, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00005.33" ], "nt": "ATGAGTTTTTTTAAAAAAAAGAATGATTTACAAAAAGAAGAACACAAACACGTTAGTGTTGTAGATATGAATGAAATTTTAAGCTATGAAGATAGTGAAGAATTTAAAAACGATGAAATTGATTCTAATGAGCCAATTTTTAAACTTAATAATGTTTCATTAGGTTACGGAAAAAAAATGATTATTAAAAACCTAACGGCAACAATTCATCGCAACGATTTTGTTGTTGTATTAGGGCCTAATGGTTCTGGTAAATCCACATTAATCAAAGGATTGTGTCGTATCAATAATCCTTCAAGTGGTTATATAATGTATAGAGATAAATTGATATCAAGACCATTATTACCATTTCTTTGA", "aa": "MSFFKKKNDLQKEEHKHVSVVDMNEILSYEDSEEFKNDEIDSNEPIFKLNNVSLGYGKKMIIKNLTATIHRNDFVVVLGPNGSGKSTLIKGLCRINNPSSGYIMYRDKLISRPLLPFL", "aa_hexdigest": "d4db79aba6596f8fd6505ad150503235", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00005.33", "name": "ABC_tran", "length": 49, "aa_cov": 0.4152542372881356, "hmm_cov": 1.0, "evalue": 1.2866897130256823e-11, "score": 40.842254638671875, "start": 63, "stop": 111 } ], "seq_stats": { "molecular_weight": 13506.419199999977, "isoelectric_point": 8.646064567565919 }, "id": "NKIECMJPJD_724", "locus": "GCA000828735_00709" }, { "type": "cds", "sequence": "contig_1", "start": 427760, "stop": 428065, "strand": "+", "frame": 2, "gene": "ureA", "product": "urease subunit gamma", "db_xrefs": [ "COG:COG0831", "COG:E", "EC:3.5.1.5", "GO:0005737", "GO:0009039", "GO:0016151", "GO:0043419", "RefSeq:WP_010891770.1", "SO:0001217", "UniParc:UPI0000137DA3", "UniRef:UniRef100_P0C7K9", "UniRef:UniRef50_Q8UCS6", "UniRef:UniRef90_P0C7K9" ], "nt": "ATGAATCTATCATTAAGAGAAGTCCAAAAATTATTGATAACAGTTGCTGCTGACGTTGCAAGAAGACGTTTAGCTAGAGGTTTAAAATTAAACTATTCAGAAGCTGTTGCTTTAATTACTGATCATGTAATGGAAGGGGCAAGAGATGGTAAGCTAGTTGCTGACCTAATGCAATCTGCTCGTGAAGTACTACGTGTTGATCAAGTTATGGAAGGTGTAGATACAATGGTTAGTATAATTCAAGTTGAAGTTACTTTCCCTGATGGTACTAAACTAGTTTCTGTACACGATCCAATTTACAAATAA", "aa": "MNLSLREVQKLLITVAADVARRRLARGLKLNYSEAVALITDHVMEGARDGKLVADLMQSAREVLRVDQVMEGVDTMVSIIQVEVTFPDGTKLVSVHDPIYK", "aa_hexdigest": "d4c1bee26cc79ea600110a2e09b83079", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI0000137DA3", "ncbi_nrp_id": "WP_010891770.1", "uniref100_id": "UniRef100_P0C7K9", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000137DA3", "RefSeq:WP_010891770.1", "UniRef:UniRef100_P0C7K9" ] }, "ips": { "uniref100_id": "UniRef100_P0C7K9", "uniref90_id": "UniRef90_P0C7K9" }, "psc": { "uniref90_id": "UniRef90_P0C7K9", "gene": "ureA", "product": "urease subunit gamma", "ec_ids": [ "3.5.1.5" ], "uniref50_id": "UniRef50_Q8UCS6", "cog_id": "COG0831", "cog_category": "E", "go_ids": [ "GO:0005737", "GO:0009039", "GO:0016151", "GO:0043419" ] }, "pscc": { "uniref50_id": "UniRef50_Q8UCS6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q8UCS6" ], "product": "Urease subunit gamma" }, "genes": [ "ureA" ], "id": "NKIECMJPJD_725", "locus": "GCA000828735_00710" }, { "type": "cds", "sequence": "contig_1", "start": 428112, "stop": 428447, "strand": "+", "frame": 3, "gene": "ureB", "product": "urease subunit beta", "db_xrefs": [ "COG:COG0832", "COG:E", "EC:3.5.1.5", "GO:0009039", "GO:0035550", "GO:0043419", "SO:0001217", "UniRef:UniRef50_P0C7K8", "UniRef:UniRef90_P0C7K8" ], "nt": "ATGTCAGGATCATCAAGTCAATTTAGTCCAGGTAAATTAGTACCAGGGGCAATTAATTTCGCTAGTGGTGAAATTGTGATGAATGAAGGTAGAGAGGCAAAAGTAATTAGTATTAAAAATACTGGGGACCGTCCTATACAAGTTGGATCACATTTTCACTTGTTTGAAGTGAATAGTGCATTAGTATTTTTTGATGAAAAAGGAAATGAAGATAAAGAACGCAAAGTTGCTTATGGACGACGTTTCGATATTCCATCAGGTACTGCTATTCGTTTTGAACCAGGAGATAAAAAAGAAGTTTCAATTATTGATTTAGCCGGAACACGCGAAGTTTGA", "aa": "MSGSSSQFSPGKLVPGAINFASGEIVMNEGREAKVISIKNTGDRPIQVGSHFHLFEVNSALVFFDEKGNEDKERKVAYGRRFDIPSGTAIRFEPGDKKEVSIIDLAGTREV", "aa_hexdigest": "af1e5422020534dcb24248128f748aeb", "start_type": "ATG", "rbs_motif": "AGGAG/GGAGG", "psc": { "uniref90_id": "UniRef90_P0C7K8", "query_cov": 1.0, "subject_cov": 0.8951612903225806, "identity": 1.0, "score": 220.0, "evalue": 1.1e-72, "valid": true, "gene": "ureB", "product": "urease subunit beta", "ec_ids": [ "3.5.1.5" ], "uniref50_id": "UniRef50_P0C7K8", "cog_id": "COG0832", "cog_category": "E", "go_ids": [ "GO:0009039", "GO:0035550", "GO:0043419" ] }, "pscc": { "uniref50_id": "UniRef50_P0C7K8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P0C7K8" ], "product": "Urease subunit beta" }, "genes": [ "ureB" ], "id": "NKIECMJPJD_726", "locus": "GCA000828735_00711" }, { "type": "cds", "sequence": "contig_1", "start": 428700, "stop": 428963, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00449.26" ], "nt": "ATGAATTCTACTATGAAGTTAGATGATAAATTAGGTAATGCTGAAGTAATGGATTTAGTTATTACAAATGCACTAATTGTTGATTATACAGGTATTTATAAAGCAGATATTGGTATTAAAAATGGAAAAATTGCTGCGATTGGTAAATCTGGAAATCCACATTTAACAGATAATGTTGATATGATTGTGGGTATCTCAACTGAAATTTCAGCTGGTGAGGGTAAAATTTATACAGCTGGTGGTTTAGATACTCACGTTCACTGA", "aa": "MNSTMKLDDKLGNAEVMDLVITNALIVDYTGIYKADIGIKNGKIAAIGKSGNPHLTDNVDMIVGISTEISAGEGKIYTAGGLDTHVH", "aa_hexdigest": "8d67e1be2709cae77a8eee147e5cc334", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00449.26", "name": "Urease_alpha", "length": 67, "aa_cov": 0.7471264367816092, "hmm_cov": 1.0, "evalue": 2.201125048169909e-25, "score": 84.91765594482422, "start": 5, "stop": 69 } ], "seq_stats": { "molecular_weight": 9130.326199999998, "isoelectric_point": 5.060000419616698 }, "id": "NKIECMJPJD_727", "locus": "GCA000828735_00712" }, { "type": "ncRNA", "class": null, "sequence": "contig_1", "start": 428710, "stop": 428979, "strand": "-", "gene": "5_ureB_sRNA", "product": "5' ureB small RNA", "score": 62.9, "evalue": 1.7e-07, "db_xrefs": [ "RFAM:RF02514", "SO:0000655" ], "nt": "CTATTTCTGGTTCTAGTCAGTGAACGTGAGTATCTAAACCACCAGCTGTATAAATTTTACCCTCACCAGCTGAAATTTCAGTTGAGATACCCACAATCATATCAACATTATCTGTTAAATGTGGATTTCCAGATTTACCAATCGCAGCAATTTTTCCATTTTTAATACCAATATCTGCTTTATAAATACCTGTATAATCAACAATTAGTGCATTTGTAATAACTAAATCCATTACTTCAGCATTACCTAATTTATCATCTAACTTCATAG", "id": "NKIECMJPJD_39", "locus": "GCA000828735_00713" }, { "type": "cds", "sequence": "contig_1", "start": 429336, "stop": 429674, "strand": "+", "frame": 3, "gene": null, "product": "amidohydrolase family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021476868" ], "nt": "ATGAAAGGGCGAACAATTCATGCTTATCATACAGAAGGTGCTGGTGGAGGGCATGCTCCAGATATTCTAGAAACTGTTAAATATGCCCATATTTTACCAGCTTCTACAAACCCAACAATTCCTTATACAGTAAATACAATTGCTGAACATTTAGATATGTTAATGGTATGTCACCACTTAAATCCTAAGGTTCCAGAAGATGTTGCTTTTGCTGATTCACGTATTCGTAGCCAAACAATTGCAGCTGAAGACTTATTGCACGATATGGGTGCAATTTCAATTATGTCATCAGATACATTAGCTATGGGACGTATTGGCGAAGTTGCAACTCGTACATGA", "aa": "MKGRTIHAYHTEGAGGGHAPDILETVKYAHILPASTNPTIPYTVNTIAEHLDMLMVCHHLNPKVPEDVAFADSRIRSQTIAAEDLLHDMGAISIMSSDTLAMGRIGEVATRT", "aa_hexdigest": "93f1bdfc3f78033ad4d79aeed2e028a3", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0021476868", "query_cov": 1.0, "subject_cov": 0.8175182481751825, "identity": 0.705, "score": 173.0, "evalue": 9.8e-54, "valid": false, "product": "amidohydrolase family protein", "uniref50_id": "UniRef50_UPI0021476868" }, "pscc": { "uniref50_id": "UniRef50_UPI0021476868", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021476868" ], "product": "amidohydrolase family protein" }, "genes": [], "id": "NKIECMJPJD_728", "locus": "GCA000828735_00714" }, { "type": "cds", "sequence": "contig_1", "start": 429678, "stop": 429854, "strand": "+", "frame": 3, "gene": null, "product": "amidohydrolase family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00374938FA" ], "nt": "ATGGCTCACAAAATGAAAGCACAATTTGGATCATTAAAAGGTGATAGTGAATTCAGTGATAACAATCGTGTAAAACGTTATATTTCTAAATATACAATTAACCCAGCTATTGCACATGGTGTTGATTCTTATATTGGTTCACTAGAAGTTGGTAAATTAGCTGATATTGTTGCTTGA", "aa": "MAHKMKAQFGSLKGDSEFSDNNRVKRYISKYTINPAIAHGVDSYIGSLEVGKLADIVA", "aa_hexdigest": "3d2094ac8b60e40703e157618b050ae4", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00374938FA", "query_cov": 0.9137931034482759, "subject_cov": 0.9814814814814815, "identity": 0.66, "score": 72.0, "evalue": 1.2e-15, "valid": false, "product": "amidohydrolase family protein", "uniref50_id": "UniRef50_UPI00374938FA" }, "pscc": { "uniref50_id": "UniRef50_UPI00374938FA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00374938FA" ], "product": "amidohydrolase family protein" }, "genes": [], "id": "NKIECMJPJD_729", "locus": "GCA000828735_00715" }, { "type": "cds", "sequence": "contig_1", "start": 429897, "stop": 430130, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGTGTAATCGCTCGTTGTGTAGCAGGTGATCCAAATGCTTCAATTCCAACATGCGAACCTGTAATTATGCGTGACCAATTTGGAACTTATGGACGTTTGTTAACTAATACATCAGTAAGTTTTGTTTCAAAAATTGGGTTAGAAAATGGCATTAAAGAGGAATATAAATTAGAAAAAGAATTATTACCAGTTAAAAATTGCCGTTCAGTAAATAAAAAGAGTATGAAATGA", "aa": "MGVIARCVAGDPNASIPTCEPVIMRDQFGTYGRLLTNTSVSFVSKIGLENGIKEEYKLEKELLPVKNCRSVNKKSMK", "aa_hexdigest": "2da4a626ff70b4370d195d9b22e46ec1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8504.880499999992, "isoelectric_point": 9.151754570007324 }, "id": "NKIECMJPJD_730", "locus": "GCA000828735_00716" }, { "type": "cds", "sequence": "contig_1", "start": 430393, "stop": 430842, "strand": "+", "frame": 1, "gene": "ureE", "product": "Urease accessory protein UreE", "db_xrefs": [ "COG:COG2371", "COG:O", "GO:0005737", "GO:0006457", "GO:0016151", "GO:0019627", "GO:0051082", "GO:0065003", "RefSeq:WP_006688775.1", "SO:0001217", "UniParc:UPI000000090C", "UniRef:UniRef100_Q56559", "UniRef:UniRef50_Q56559", "UniRef:UniRef90_Q56559" ], "nt": "TTGACTGTATTTAAAGAAATTTTAGATAATATTGCTAACATTAAAAATGTTGAAAGTTATCAAATTGAGAACATTCATTTAACAAGCGACGACGTTTTAAAACGTGTAATTATCATTTCATCAGATCAAAATGTTGAATATGGAATTCGTTTAGAAGAGGACAAAAAATTAATGGATGGTGACATCTTATACAAGGATGATTATAAACTAGTTGTTATTAGATTAGAGTTATCAGATGTATTAATTATTACAGCACACACAATTGGTGAAATGGCAAAAATTGCCCATAATTTAGGTAATCGTCATATGCCGGCTCAATTTACTGAAACACAAATGATCGTTCCCTACGACTATTTAGTAGAAGAATACCTTCAAGATAATAAAGCCCTATATGAAAGAAAAAAAATTAAACTTAAAGAAGCATTTAAACACTGTAGTGACGCTAAATAG", "aa": "MTVFKEILDNIANIKNVESYQIENIHLTSDDVLKRVIIISSDQNVEYGIRLEEDKKLMDGDILYKDDYKLVVIRLELSDVLIITAHTIGEMAKIAHNLGNRHMPAQFTETQMIVPYDYLVEEYLQDNKALYERKKIKLKEAFKHCSDAK", "aa_hexdigest": "173e1a5c166619171c4642ed5e321a09", "start_type": "TTG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI000000090C", "ncbi_nrp_id": "WP_006688775.1", "uniref100_id": "UniRef100_Q56559", "db_xrefs": [ "SO:0001217", "UniParc:UPI000000090C", "RefSeq:WP_006688775.1", "UniRef:UniRef100_Q56559" ] }, "ips": { "uniref100_id": "UniRef100_Q56559", "uniref90_id": "UniRef90_Q56559" }, "psc": { "uniref90_id": "UniRef90_Q56559", "gene": "ureE", "product": "Urease accessory protein UreE", "uniref50_id": "UniRef50_Q56559", "cog_id": "COG2371", "cog_category": "O", "go_ids": [ "GO:0005737", "GO:0016151", "GO:0051082", "GO:0006457", "GO:0065003", "GO:0019627" ] }, "pscc": { "uniref50_id": "UniRef50_Q56559", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q56559" ], "product": "Urease accessory protein UreE" }, "genes": [ "ureE" ], "id": "NKIECMJPJD_731", "locus": "GCA000828735_00717" }, { "type": "cds", "sequence": "contig_1", "start": 430784, "stop": 431077, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGAAAAAAAATTAAACTTAAAGAAGCATTTAAACACTGTAGTGACGCTAAATAGTGACTATTTAAATTTATTAGACCTAATGCAGATCACTAACGCAAACTTTCCAATTGGAACATTTAGTCATTCTTTTGGAGTTGAAACTTATATTAGAAAAGATATTGTTTTTGATGGAGAATCATTAATTAAAGCGTTACTTCTGTATATGAATGAACAATTGTTACATGGTGATTTATTGGCAATTTATCAAATTTTTAAACTATTGCCAAAACAAAAAATAAATGCAATTTGA", "aa": "MKEKKLNLKKHLNTVVTLNSDYLNLLDLMQITNANFPIGTFSHSFGVETYIRKDIVFDGESLIKALLLYMNEQLLHGDLLAIYQIFKLLPKQKINAI", "aa_hexdigest": "33df8464cf85b9a707be397b1943738c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11179.077399999987, "isoelectric_point": 8.893365287780764 }, "id": "NKIECMJPJD_732", "locus": "GCA000828735_00718" }, { "type": "cds", "sequence": "contig_1", "start": 431090, "stop": 431536, "strand": "+", "frame": 2, "gene": "ureF", "product": "Urease accessory protein UreF", "db_xrefs": [ "GO:0005737", "GO:0016151", "KEGG:K03188", "SO:0001217", "UniRef:UniRef50_Q56560", "UniRef:UniRef90_Q56560" ], "nt": "ATGATAAACTTTCAAGGTTTAGCAAGAGAGACTCGTGAAGGTCAACGTCGAATTGGACAACAAATGGTAAAGATATATAATGAACTTTTTAATTGTGAACTTTTAGTTGAATATGCTGAAAGAATAAAAAATAAAAAATCTTATGGTAATCCTGCTGTTGCGTTTGCTTTATTAGCTATGCATTTAAAAATAGATTTAAAAACTGCTTTATATACGCATCTTTACTCTACAGTTGCTGCATTAACGCAAAACTGTGTACGTGCAATTCCATTAGGACAAGTTAAGGGACAAAAAATAATTTATCAACTTAAACATGTTTATTTTGATGATATTGTTAATAAAGTCTTTACATTGGATTTTAAAACAGATTTTTGCAAAAATATACCAGGCCTTGAAATTGCACAAATGGAACACGAAGACACACCTGTTCGATTATTCATGTCATAA", "aa": "MINFQGLARETREGQRRIGQQMVKIYNELFNCELLVEYAERIKNKKSYGNPAVAFALLAMHLKIDLKTALYTHLYSTVAALTQNCVRAIPLGQVKGQKIIYQLKHVYFDDIVNKVFTLDFKTDFCKNIPGLEIAQMEHEDTPVRLFMS", "aa_hexdigest": "b0b677bfc303a8984861792e180cbc90", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "ureF" ], "truncated": "5-prime", "pseudogene": { "start": 430790, "stop": 431536, "observations": { "mutation": [ 431075 ], "stop": [ 431075 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q56560", "query_cov": 1.0, "subject_cov": 0.592, "identity": 1.0, "score": 299.0, "evalue": 2.89e-101, "gene_start": 1, "gene_end": 148, "reference_start": 103, "reference_end": 250, "reference_sequence": "MKEKKLNLKKHLNTVVTLNSDYLNLLDLMQITNANFPIGTFSHSFGVETYIRKDIVFDGESLIKALLLYMNEQLLHGDLLAIYQIFKLLPKQKINAIWEIDQMINFQGLARETREGQRRIGQQMVKIYNELFNCELLVEYAERIKNKKSYGNPAVAFALLAMHLKIDLKTALYTHLYSTVAALTQNCVRAIPLGQVKGQKIIYQLKHVYFDDIVNKVFTLDFKTDFCKNIPGLEIAQMEHEDTPVRLFMS" }, "paralog": false, "identity": 0.9959677419354839, "score": 489.0, "evalue": 8.74e-179, "description": "Internal stop codon at 431075. Nonsense mutation around 431075.", "psc": { "uniref90_id": "UniRef90_Q56560", "gene": "ureF", "product": "Urease accessory protein UreF", "uniref50_id": "UniRef50_Q56560", "kegg_orthology_id": "K03188", "go_ids": [ "GO:0005737", "GO:0016151" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q56560", "UniRef:UniRef50_Q56560", "KEGG:K03188", "GO:0005737", "GO:0016151" ] }, "pscc": { "uniref50_id": "UniRef50_Q56560", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q56560" ], "product": "Urease accessory protein UreF" } }, "id": "NKIECMJPJD_733", "locus": "GCA000828735_00719" }, { "type": "cds", "sequence": "contig_1", "start": 431618, "stop": 432199, "strand": "+", "frame": 2, "gene": "ureG", "product": "urease accessory protein UreG", "db_xrefs": [ "COG:COG0378", "COG:O", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0016151", "GO:0016530", "GO:0019627", "GO:0043419", "GO:0046872", "KEGG:K03189", "SO:0001217", "UniRef:UniRef50_B1XYX6", "UniRef:UniRef90_Q56561" ], "nt": "ATGAAAAGACCATTAATTATTGGTGTAGGTGGACCTGTAGGAGCAGGTAAAACAATGCTAATCGAAAGATTAACAAGATACCTTTCAACAAAAGGTTATAGTATGGCAGCAATTACTAATGATATTTACACTAAAGAAGATGCTAGAATTTTATTAAATACTTCTGTTTTACCAGCTGATCGTATTGCTGGTGTTGAAACAGGAGGATGTCCACATACAGCAATTCGTGAAGATGCTTCAATGAACTTTGCTGCAATTGAAGAAATGTGTGATAAACACCCTGATTTACAATTATTATTTTTAGAATCTGGTGGGGATAATTTATCTGCAACATTTAGTCCAGATTTAGTTGATTTTTCAATTTATATCATTGATGTTGCACAAGGAGAAAAAATTCCTCGTAAGGGTGGTCAAGGAATGATTAAATCAGACTTATTTATTATTAATAAAGTTGATTTAGCTCCTTATGTTGGTGCTAATGTAGAAGTAATGAAAGCCGATACATTAAAATCACGTGGTAATAAAGATTTCTTTGTAACGAATTTAAAAACAGATGAAGGTTTAAAATCTGTTGCTGATTGA", "aa": "MKRPLIIGVGGPVGAGKTMLIERLTRYLSTKGYSMAAITNDIYTKEDARILLNTSVLPADRIAGVETGGCPHTAIREDASMNFAAIEEMCDKHPDLQLLFLESGGDNLSATFSPDLVDFSIYIIDVAQGEKIPRKGGQGMIKSDLFIINKVDLAPYVGANVEVMKADTLKSRGNKDFFVTNLKTDEGLKSVAD", "aa_hexdigest": "376ee03b3e0b6332264fba5f0245eb77", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_Q56561", "query_cov": 1.0, "subject_cov": 0.9368932038834952, "identity": 1.0, "score": 384.0, "evalue": 8.3e-135, "valid": true, "gene": "ureG", "product": "urease accessory protein UreG", "uniref50_id": "UniRef50_B1XYX6", "cog_id": "COG0378", "cog_category": "O", "kegg_orthology_id": "K03189", "go_ids": [ "GO:0003924", "GO:0005525", "GO:0005737", "GO:0016151", "GO:0016530", "GO:0019627", "GO:0043419", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_B1XYX6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B1XYX6" ], "product": "Urease accessory protein UreG" }, "genes": [ "ureG" ], "id": "NKIECMJPJD_734", "locus": "GCA000828735_00720" }, { "type": "cds", "sequence": "contig_1", "start": 432248, "stop": 432712, "strand": "+", "frame": 2, "gene": "ureD", "product": "Urease accessory protein UreD", "db_xrefs": [ "COG:COG0829", "COG:O", "GO:0005737", "GO:0016151", "KEGG:K03190", "SO:0001217", "UniRef:UniRef50_Q56562", "UniRef:UniRef90_Q56562" ], "nt": "ATGATTTTAAATAAAGAAAAAATTAAAAATTATGCTGCTTATTTATACATTAAAGTAGCATATGATCAAGCTCATTCAAAAATGGCACATACTGTCTATTTTACGAATTTTTATCGTTCATCTAAACCTTTATTTTTAGATGAAGAAGATCCAATCAACCCCTGTTTTCAAACAATTAGTATGGGTGGTGGATATGTTTCAGGTGAAATTTATCGTTCTGATTTTGAAATTAATGATGATGCTCGTTGTATCATTACAACACAATCCTCAGCTAAAGCTTATAAAACGGTTGATGGAAAAACTTCTGAGCAACATACAAATATCACGTTAGGAAAAAATAGTATTTTAGAATATATAAGTGATAACGTAATTGTTTATGAAGATGGAAAGTTCGCACAATTTAATAATTTTAAAATGGATTCAAGTGCTACTTTAATTTACACAGAATGTTTTGGCCCTGGTTGA", "aa": "MILNKEKIKNYAAYLYIKVAYDQAHSKMAHTVYFTNFYRSSKPLFLDEEDPINPCFQTISMGGGYVSGEIYRSDFEINDDARCIITTQSSAKAYKTVDGKTSEQHTNITLGKNSILEYISDNVIVYEDGKFAQFNNFKMDSSATLIYTECFGPG", "aa_hexdigest": "207b417fe4ca05f1d3e94d704259cfcf", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "genes": [ "ureD" ], "truncated": "3-prime", "pseudogene": { "start": 432248, "stop": 433015, "observations": { "mutation": [ 432710 ], "stop": [ 432710 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q56562", "query_cov": 1.0, "subject_cov": 0.5365853658536586, "identity": 1.0, "score": 315.0, "evalue": 5.26e-107, "gene_start": 1, "gene_end": 154, "reference_start": 1, "reference_end": 154, "reference_sequence": "MILNKEKIKNYAAYLYIKVAYDQAHSKMAHTVYFTNFYRSSKPLFLDEEDPINPCFQTISMGGGYVSGEIYRSDFEINDDARCIITTQSSAKAYKTVDGKTSEQHTNITLGKNSILEYISDNVIVYEDGKFAQFNNFKMDSSATLIYTECFGPGWSPHGSAYQYEKMYLNTKIYYDDKLVLFDNLKFQPRKNDESAFGIMDGYHYCGTMIVINQQVIEDDVIKIRDLVKEKYPDMDMIFGVSRMDIPGLGLRVLANTYYHVEKINAVAHDYFRRKLFNKKPLILRKP" }, "paralog": false, "identity": 0.996078431372549, "score": 519.0, "evalue": 8.04e-190, "description": "Internal stop codon at 432710. Nonsense mutation around 432710.", "psc": { "uniref90_id": "UniRef90_Q56562", "gene": "ureD", "product": "Urease accessory protein UreD", "uniref50_id": "UniRef50_Q56562", "cog_id": "COG0829", "cog_category": "O", "kegg_orthology_id": "K03190", "go_ids": [ "GO:0005737", "GO:0016151" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q56562", "UniRef:UniRef50_Q56562", "COG:COG0829", "COG:O", "KEGG:K03190", "GO:0005737", "GO:0016151" ] }, "pscc": { "uniref50_id": "UniRef50_Q56562", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q56562" ], "product": "Urease accessory protein UreD" } }, "id": "NKIECMJPJD_735", "locus": "GCA000828735_00721" }, { "type": "cds", "sequence": "contig_1", "start": 432746, "stop": 433111, "strand": "+", "frame": 2, "gene": "ureD", "product": "Urease accessory protein UreD", "db_xrefs": [ "COG:COG0829", "COG:O", "GO:0005737", "GO:0016151", "KEGG:K03190", "SO:0001217", "UniRef:UniRef50_Q56562", "UniRef:UniRef90_Q56562" ], "nt": "ATGTATTTAAATACTAAAATATATTATGATGATAAATTGGTTTTATTTGATAATTTAAAATTTCAACCACGTAAAAATGATGAATCAGCATTTGGAATCATGGATGGCTATCATTATTGTGGAACCATGATTGTAATTAATCAACAAGTTATTGAAGATGATGTTATTAAAATTCGTGATTTAGTTAAGGAAAAATATCCTGACATGGATATGATATTTGGTGTATCACGTATGGATATTCCTGGTTTGGGATTACGAGTTTTAGCTAATACTTATTATCATGTTGAAAAAATTAATGCTGTTGCTCATGATTATTTTAGAAGAAAATTATTCAATAAAAAACCTTTAATTTTACGAAAACCATAA", "aa": "MYLNTKIYYDDKLVLFDNLKFQPRKNDESAFGIMDGYHYCGTMIVINQQVIEDDVIKIRDLVKEKYPDMDMIFGVSRMDIPGLGLRVLANTYYHVEKINAVAHDYFRRKLFNKKPLILRKP", "aa_hexdigest": "04e6261a7ffed0f5bcd48cbd34a35b2d", "start_type": "ATG", "rbs_motif": null, "genes": [ "ureD" ], "truncated": "5-prime", "pseudogene": { "start": 432446, "stop": 433111, "observations": { "mutation": [ 432710 ], "stop": [ 432710 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q56562", "query_cov": 1.0, "subject_cov": 0.42160278745644597, "identity": 1.0, "score": 251.0, "evalue": 2.32e-82, "gene_start": 1, "gene_end": 121, "reference_start": 167, "reference_end": 287, "reference_sequence": "MILNKEKIKNYAAYLYIKVAYDQAHSKMAHTVYFTNFYRSSKPLFLDEEDPINPCFQTISMGGGYVSGEIYRSDFEINDDARCIITTQSSAKAYKTVDGKTSEQHTNITLGKNSILEYISDNVIVYEDGKFAQFNNFKMDSSATLIYTECFGPGWSPHGSAYQYEKMYLNTKIYYDDKLVLFDNLKFQPRKNDESAFGIMDGYHYCGTMIVINQQVIEDDVIKIRDLVKEKYPDMDMIFGVSRMDIPGLGLRVLANTYYHVEKINAVAHDYFRRKLFNKKPLILRKP" }, "paralog": false, "identity": 0.995475113122172, "score": 449.0, "evalue": 5.65e-163, "description": "Internal stop codon at 432710. Nonsense mutation around 432710.", "psc": { "uniref90_id": "UniRef90_Q56562", "gene": "ureD", "product": "Urease accessory protein UreD", "uniref50_id": "UniRef50_Q56562", "cog_id": "COG0829", "cog_category": "O", "kegg_orthology_id": "K03190", "go_ids": [ "GO:0005737", "GO:0016151" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q56562", "UniRef:UniRef50_Q56562", "COG:COG0829", "COG:O", "KEGG:K03190", "GO:0005737", "GO:0016151" ] }, "pscc": { "uniref50_id": "UniRef50_Q56562", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q56562" ], "product": "Urease accessory protein UreD" } }, "id": "NKIECMJPJD_736", "locus": "GCA000828735_00722" }, { "type": "cds", "sequence": "contig_1", "start": 433218, "stop": 433592, "strand": "+", "frame": 3, "gene": "ftnA", "product": "putative protein UU427", "db_xrefs": [ "COG:COG1528", "COG:P", "GO:0008199", "SO:0001217", "UniRef:UniRef50_Q56564", "UniRef:UniRef90_Q9PQ61" ], "nt": "ATGGTTAAATCTCAAAAAGTTATAGATGTTTTAAATACACATTATAATTTAAATTTAGAACTAGGTAGTATTTATGCACAATATGCTCATATTGCTGATGACCAATTCAGTATGCCTTTTTTAGCAAAATTTATTGCTGATTTAAGCAATGATAAATTAGGTGTTCACAAGAATTTAATTTCTGAATATGCTCGTAAAGTTGAAATTCCATTACACACTAAATTTAGTGTAGATGTTAATTTTAAGCCAGCGAATCCTAAGGAATTAATAAAACACATTTTAGATACAGAATTAAAAGTTCGTAAGCATGTAGCCAATATGGCAAAAGTATGTTTGGAAGAAAATGATTTTGAAACATTTAGTTTTGTAAAATGA", "aa": "MVKSQKVIDVLNTHYNLNLELGSIYAQYAHIADDQFSMPFLAKFIADLSNDKLGVHKNLISEYARKVEIPLHTKFSVDVNFKPANPKELIKHILDTELKVRKHVANMAKVCLEENDFETFSFVK", "aa_hexdigest": "54b29358093e8bc9698bc185ad99919b", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [ "ftnA" ], "truncated": "3-prime", "pseudogene": { "start": 433218, "stop": 433730, "observations": { "mutation": [ 433590 ], "stop": [ 433590 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQ61", "query_cov": 1.0, "subject_cov": 0.7294117647058823, "identity": 1.0, "score": 249.0, "evalue": 4.54e-83, "gene_start": 1, "gene_end": 124, "reference_start": 1, "reference_end": 124, "reference_sequence": "MVKSQKVIDVLNTHYNLNLELGSIYAQYAHIADDQFSMPFLAKFIADLSNDKLGVHKNLISEYARKVEIPLHTKFSVDVNFKPANPKELIKHILDTELKVRKHVANMAKVCLEENDFETFSFVKWFVDDGIKDFDDVRTIHDFFENATNNLQVEYAIRKYLKQMKVEEEK" }, "paralog": false, "identity": 0.9941176470588236, "score": 338.0, "evalue": 6.41e-121, "description": "Internal stop codon at 433590. Nonsense mutation around 433590.", "psc": { "uniref90_id": "UniRef90_Q9PQ61", "gene": "FtnA", "product": "Uncharacterized protein UU427", "uniref50_id": "UniRef50_Q56564", "cog_id": "COG1528", "cog_category": "P", "go_ids": [ "GO:0008199" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQ61", "UniRef:UniRef50_Q56564", "COG:COG1528", "COG:P", "GO:0008199" ] }, "pscc": { "uniref50_id": "UniRef50_Q56564", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q56564" ], "product": "Uncharacterized protein UU427 homolog" } }, "id": "NKIECMJPJD_737", "locus": "GCA000828735_00723" }, { "type": "cds", "sequence": "contig_1", "start": 434422, "stop": 434682, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCTAAATCTTATAAAATTCGCACAAATTATGCAAGCGTACTAAATTCACTAAATTTATCATTAGATGCTAAAAATCAATTTAAAAAAAATTTTGAGCACACTTTTAGAATATACAATAAAACATTTTTAGATTTTATTATTGATGGTAAACAATACTATGATCCTAAAAAGCAAAACTTAATTTTTATGGGTTCTTCACTATTTAAAAATAATGTTTTAAAAGATCGTAAAATTGCTAAACAAGAATTACAACAATGA", "aa": "MPKSYKIRTNYASVLNSLNLSLDAKNQFKKNFEHTFRIYNKTFLDFIIDGKQYYDPKKQNLIFMGSSLFKNNVLKDRKIAKQELQQ", "aa_hexdigest": "21364eebe515d6bc75cee02040553687", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10270.757999999994, "isoelectric_point": 9.900941658020017 }, "id": "NKIECMJPJD_738", "locus": "GCA000828735_00724" }, { "type": "cds", "sequence": "contig_1", "start": 435118, "stop": 435225, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAACTAGCAATGATTTACTAATTTATTCTAATTTAAAACAAACACCTAAATACCAAATAGATAATAAAAAACTAAAAGAAGATTTTGTTAAAAATAATAATTGA", "aa": "MATSNDLLIYSNLKQTPKYQIDNKKLKEDFVKNNN", "aa_hexdigest": "7b358d5cce3fb963ad7a649d9c32e178", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4129.647600000002, "isoelectric_point": 9.158330345153807 }, "id": "NKIECMJPJD_739", "locus": "GCA000828735_00725" }, { "type": "cds", "sequence": "contig_1", "start": 435831, "stop": 436640, "strand": "-", "frame": 1, "gene": null, "product": "DUF3196 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1C63", "UniRef:UniRef100_A0A2C9DYK7", "UniRef:UniRef50_A0A2C9DYK7", "UniRef:UniRef90_A0A2C9DYK7" ], "nt": "ATGGTTTGCATCATTTTAAAAATAAAAAAGGGAATGTTAAAGATAATGAATAATAATAAAAGCTACATTAAAAAATATTTTGATGATATTTTAATACAGGCTAAACAGGTTTTAAAAAAACATGATTATGCGTATGCATATGATTTAATTTCTAATGAATTTAATAATCCATTAATTGATTTAAAAACTTTACAAGAATTTGAAGCATTTGCTATAGAAATTAAAAAAAGTGCCGAACTTAACTTTATTGATGAAAATGAGGCAAAATTAGCTAAAACAGATTTTTATTGTAAAATTCATGATCCAAAAACAACTTATGTTAGTTTAGCATATTTAGAAACTTTTTTAATGCGTTTCATTAATGAAATGGATGAATTAGATATTACATTTTTAAATAGCTTGCTAACAAATAAATTAATTAATGGATCAACAAAATTAGATATTTTAGATTTATTAGCAACTAATAATATTGATCAAAATTTTAATTTTTACAATAAATATTTAAAACAAACAAAAAATGTTAACCCTGTTAATTTTAATAACCATTACCAAGAAATTGACCAAATTCAAAATTATCTAAATAAGTATTTAGCTAAAAATCCTTCTTTATTACATTTAGCCAATAAATTATTAATGATGTACATTACATATTATTTTCCATTTAAATTTGCACATAATCATAATTTAATTGCTAAAACAATTATTGATTATGTAAAAAATGCTATGGAAAATCATGTTTGTCAGTATAGTGAAGAACAAAAAGCTATTGTGGCTTGTATTAATAAAATTTTAGAAGAAGAGCAAGTATAA", "aa": "MVCIILKIKKGMLKIMNNNKSYIKKYFDDILIQAKQVLKKHDYAYAYDLISNEFNNPLIDLKTLQEFEAFAIEIKKSAELNFIDENEAKLAKTDFYCKIHDPKTTYVSLAYLETFLMRFINEMDELDITFLNSLLTNKLINGSTKLDILDLLATNNIDQNFNFYNKYLKQTKNVNPVNFNNHYQEIDQIQNYLNKYLAKNPSLLHLANKLLMMYITYYFPFKFAHNHNLIAKTIIDYVKNAMENHVCQYSEEQKAIVACINKILEEEQV", "aa_hexdigest": "57bb5c20ea82b7dc40b1890d00d04094", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00000C1C63", "uniref100_id": "UniRef100_A0A2C9DYK7", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1C63", "UniRef:UniRef100_A0A2C9DYK7" ] }, "ips": { "uniref100_id": "UniRef100_A0A2C9DYK7", "uniref90_id": "UniRef90_A0A2C9DYK7" }, "psc": { "uniref90_id": "UniRef90_A0A2C9DYK7", "product": "DUF3196 domain-containing protein", "uniref50_id": "UniRef50_A0A2C9DYK7" }, "pscc": { "uniref50_id": "UniRef50_A0A2C9DYK7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2C9DYK7" ], "product": "DUF3196 domain-containing protein" }, "genes": [], "id": "NKIECMJPJD_740", "locus": "GCA000828735_00726" }, { "type": "cds", "sequence": "contig_1", "start": 436675, "stop": 436833, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTCAAAAAATTTTAAGCGTTGAAACTAAAATTGAAAATTATATTAAAGAAGGTTATTTTCGTAAACTTGTCACTCGTCAATTATTGATTAAACTACACGTTTTAGATGATATTGATGATTTTAAAACTAATTTTAATAAACACACTAATGAATGA", "aa": "MIQKILSVETKIENYIKEGYFRKLVTRQLLIKLHVLDDIDDFKTNFNKHTNE", "aa_hexdigest": "6510b2cf80313e00900e051fa8267aab", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6294.238, "isoelectric_point": 8.051214790344236 }, "id": "NKIECMJPJD_741", "locus": "GCA000828735_00727" }, { "type": "cds", "sequence": "contig_1", "start": 436894, "stop": 437064, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTTATTGGTTCTTTTTTAAAAATGAGTGGTTTAATTTAATTGCAACGGTTGAAAATGATGGTATCAAGTTTTATATTAATGTTGCATCTCCTTTTATTTATGAAGAGCAAGCAGTTAAGTATTATGATTTTGATTTAGATTTTAAAATTTCATCAGATGATAAATGA", "aa": "MSYWFFFKNEWFNLIATVENDGIKFYINVASPFIYEEQAVKYYDFDLDFKISSDDK", "aa_hexdigest": "18d0871c2a9c9dd31661d92781ebbe2c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6853.578500000001, "isoelectric_point": 4.2393590927124025 }, "id": "NKIECMJPJD_742", "locus": "GCA000828735_00728" }, { "type": "cds", "sequence": "contig_1", "start": 437197, "stop": 437298, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAGAAAAAAAACAAACAAAGAATGAAAATTTTCAAGATCTAGATCAATTAAAAATTGGTGATGAACTAATGGTGCATGCTTATAAACTTAATGGTTGA", "aa": "MQEKKQTKNENFQDLDQLKIGDELMVHAYKLNG", "aa_hexdigest": "f6c1f542bd7ac05f70837bfd1a1aae8a", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3907.3872999999994, "isoelectric_point": 5.564105415344237 }, "id": "NKIECMJPJD_743", "locus": "GCA000828735_00729" }, { "type": "cds", "sequence": "contig_1", "start": 437310, "stop": 437516, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T3H6", "UniRef:UniRef90_A0AAC9T3H6" ], "nt": "TTGAATAAGCAATTGGAACAAATATATTTGAAATACATAATTTTTTATTGGTATAACATTAATATTCCAATAAATTATGAAATTGATGATTTTAATATTATTTGTTTACGCAATAAAGCATTTGTAGATTGTATGCAAATTTTACAAGAATATTATCATATTAATGTTGATATTTTAATTAATTTAGTTATCAAACAAATTAGATAA", "aa": "MNKQLEQIYLKYIIFYWYNINIPINYEIDDFNIICLRNKAFVDCMQILQEYYHINVDILINLVIKQIR", "aa_hexdigest": "a48b86ed9aa72feef5c4ead13195e46a", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 437310, "stop": 437813, "observations": { "mutation": [ 437570, 437654 ], "stop": [ 437570, 437654 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAC9T3H6", "query_cov": 1.0, "subject_cov": 0.40718562874251496, "identity": 0.985, "score": 136.0, "evalue": 1.15e-39, "gene_start": 1, "gene_end": 68, "reference_start": 100, "reference_end": 167, "reference_sequence": "MNIKKIKREFNTHYELIKKEIKNLNEYKKELEFLIATIYFNSKMVFKEISLDSWKKFSDYVYRFFLIINKNNQSQNFDLDCWYLEIKELIKVLNISNFLLNKQLEQIYLKYIIFYWYNINIPINYEIDDFNIICLRNKAFVDCMQILQEYYHINVDILINLVIKQIR" }, "paralog": false, "identity": 0.9880239520958084, "score": 331.0, "evalue": 5.53e-119, "description": "Internal stop codon at 437570, 437654. Nonsense mutation around 437570, 437654.", "psc": { "uniref90_id": "UniRef90_A0AAC9T3H6", "uniref50_id": "UniRef50_A0AAC9T3H6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAC9T3H6", "UniRef:UniRef50_A0AAC9T3H6" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAC9T3H6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T3H6" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 8461.890799999997, "isoelectric_point": 5.586613655090332 }, "id": "NKIECMJPJD_744", "locus": "GCA000828735_00730" }, { "type": "cds", "sequence": "contig_1", "start": 437652, "stop": 437813, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACATTAAAAAAATTAAACGTGAATTTAATACTCATTACGAATTGATTAAAAAAGAAATTAAAAACTTAAATGAATATAAAAAAGAATTAGAATTCTTAATTGCAACAATTTATTTTAATTCTAAAATGGTTTTTAAAGAAATATCATTAGATAGTTGA", "aa": "MNIKKIKREFNTHYELIKKEIKNLNEYKKELEFLIATIYFNSKMVFKEISLDS", "aa_hexdigest": "de1a08700e112986ac3bba80e86550e0", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6514.608299999998, "isoelectric_point": 9.26070613861084 }, "id": "NKIECMJPJD_745", "locus": "GCA000828735_00731" }, { "type": "cds", "sequence": "contig_1", "start": 437803, "stop": 437988, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTAGGAAATGAAATTGGCCACGAACATTTAGCCATTGCAACAACTCAAATTAATTTAGCAAACACTAATCATCAAATTACCGTTGTAGGTCCAACAAGAATGGATTATGCAAAAATCAAAGCTTTATTAGATTTTTTAAAAATTGAAATTGAAAAAATTTTAGGATCAACAAATGAACATTAA", "aa": "MLGNEIGHEHLAIATTQINLANTNHQITVVGPTRMDYAKIKALLDFLKIEIEKILGSTNEH", "aa_hexdigest": "29e3d29cbd33c1a35078f474d9531765", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6780.739199999998, "isoelectric_point": 6.008870506286621 }, "id": "NKIECMJPJD_746", "locus": "GCA000828735_00732" }, { "type": "cds", "sequence": "contig_1", "start": 438034, "stop": 438876, "strand": "-", "frame": 3, "gene": "hrcA", "product": "heat-inducible transcriptional repressor HrcA", "db_xrefs": [ "COG:COG1420", "COG:K", "GO:0003677", "GO:0006355", "GO:0045892", "KEGG:K03705", "SO:0001217", "UniRef:UniRef50_Q9PQ68", "UniRef:UniRef90_Q9PQ68" ], "nt": "ATGAAGAATACTAAAACATCGCAAGAACAAGTAATAGAATTAAAATTAAGTGATCGGCAAAAAAAAGTATTAAAATCTATCGTCGATGAATACACTATTAGTGCAACACCGGTCTCTTCAAAACTTTTAGTACAAAAAGAATTCAAAGATCAATCTTCTGCTACAATTCGTAACGAAATGATGTTTCTTGAAAAAAACGGATTAATTGAAAAACAGCACATTAGTGGTGGGCGTGTTCCATCACTAAAAGGTTATGATTTTTATAATAAAAATTTAATTAATAAAAATAATAATGTTTCTGATAATTTTAAAATGCGTTTACATAAAATCCTTAGTAAACGATATTCCAATATTGATGAAATTTTAAATGCTGCTGTAAGTATCATTAATGAAACAACCCAATTACCAGCTGTTGTTACAAAAACAAGTAGTGATGAATTATTAAAACGAATTGATTTAGTAAAAATCAATGATAATTCGGCGTTAGTGTTAATTGTAACTTCAAGCGAAAACATTTTAACTCATTCAATTAAGCTTGATAAAAACACAAAATTTAATGATTTACAAACGTGTTTTAGTGTTTTAGATGAACGATTAGTTGATACTAAACTTAGTTTAATCAGTTCTAAATTAGATTTAATTGTCGATATAGTTCGTAATAAACTTGAAGAAGTAGAATACTATTTTAGTAAAATTGTACATCGTGTTTTTGATTTTTACGCTTATAAACCTAACATTGATTCTAAAACTTATGGAGTTAAATACTTAACTAAACATTCTGAATTTGAAGATCAACAAAAACTAAATGATTTACTAAATTTATTAGAAGACAGTACAATTTGA", "aa": "MKNTKTSQEQVIELKLSDRQKKVLKSIVDEYTISATPVSSKLLVQKEFKDQSSATIRNEMMFLEKNGLIEKQHISGGRVPSLKGYDFYNKNLINKNNNVSDNFKMRLHKILSKRYSNIDEILNAAVSIINETTQLPAVVTKTSSDELLKRIDLVKINDNSALVLIVTSSENILTHSIKLDKNTKFNDLQTCFSVLDERLVDTKLSLISSKLDLIVDIVRNKLEEVEYYFSKIVHRVFDFYAYKPNIDSKTYGVKYLTKHSEFEDQQKLNDLLNLLEDSTI", "aa_hexdigest": "d3959ce7a6fe1ad2a50335f7d751f86f", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "genes": [ "hrcA" ], "truncated": "5-prime", "pseudogene": { "start": 437803, "stop": 438876, "observations": { "mutation": [ 438036 ], "stop": [ 438036 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQ68", "query_cov": 1.0, "subject_cov": 0.7843137254901961, "identity": 1.0, "score": 527.0, "evalue": 1.35e-187, "gene_start": 1, "gene_end": 280, "reference_start": 1, "reference_end": 280, "reference_sequence": "MKNTKTSQEQVIELKLSDRQKKVLKSIVDEYTISATPVSSKLLVQKEFKDQSSATIRNEMMFLEKNGLIEKQHISGGRVPSLKGYDFYNKNLINKNNNVSDNFKMRLHKILSKRYSNIDEILNAAVSIINETTQLPAVVTKTSSDELLKRIDLVKINDNSALVLIVTSSENILTHSIKLDKNTKFNDLQTCFSVLDERLVDTKLSLISSKLDLIVDIVRNKLEEVEYYFSKIVHRVFDFYAYKPNIDSKTYGVKYLTKHSEFEDQQKLNDLLNLLEDSTIWQQIALNKKTDGLAKIMLGNEIGHEHLAIATTQINLANTNHQITVVGPTRMDYAKIKALLDFLKIEIEKILGSTNEH" }, "paralog": false, "identity": 0.9971988795518207, "score": 686.0, "evalue": 1.68e-252, "description": "Internal stop codon at 438036. Nonsense mutation around 438036.", "psc": { "uniref90_id": "UniRef90_Q9PQ68", "gene": "hrcA", "product": "heat-inducible transcriptional repressor HrcA", "uniref50_id": "UniRef50_Q9PQ68", "cog_id": "COG1420", "cog_category": "K", "kegg_orthology_id": "K03705", "go_ids": [ "GO:0003677", "GO:0006355", "GO:0045892" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQ68", "UniRef:UniRef50_Q9PQ68", "COG:COG1420", "COG:K", "KEGG:K03705", "GO:0003677", "GO:0006355", "GO:0045892" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQ68", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQ68" ], "product": "Heat-inducible transcription repressor HrcA" } }, "id": "NKIECMJPJD_747", "locus": "GCA000828735_00733" }, { "type": "cds", "sequence": "contig_1", "start": 439097, "stop": 439609, "strand": "-", "frame": 2, "gene": null, "product": "DUF3137 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T0Q2", "UniRef:UniRef90_A0AAC9T0Q2" ], "nt": "ATGAAAACAATAAATTTAAAAAAAGAATTTAATCAATATTGTATTAGTTTAAAAACTCAAGATCTTATTAAACAATACTTTAATGATGAAAAATTAAAAGAAAAATTATTAAAAAATCGTTTAATTGGATTAATTAGTTTAATTATCATTTTTATAATTGGTGTGGGAATTTTAATTCTTGGTGCTTTTATTAATAAAGGTAATGGCGCTAATTTAAATAATGGGGCAATAACTTGTGTTATCATAGGAATTTTCTTTTTGTTATGTTTATTAATTCCTTACACTACTGTTGCAAATCAAAATAAAAAAATTATGCATAAATATATTGAATATCATTTTAATGAAGTTAATGTTGGACAACTAATTAAAAAAATAAGTACTTTTTTTCAAGAATTTAGGTTATTAACAGAAGGTTTTTTAAATTTTAAACAAAGTAATCAAGTTATTGATATCAATTATTGTAATGAAAAATATAGTTTAATTTATTCATTCCAACAAAAACCACAAGTATGA", "aa": "MKTINLKKEFNQYCISLKTQDLIKQYFNDEKLKEKLLKNRLIGLISLIIIFIIGVGILILGAFINKGNGANLNNGAITCVIIGIFFLLCLLIPYTTVANQNKKIMHKYIEYHFNEVNVGQLIKKISTFFQEFRLLTEGFLNFKQSNQVIDINYCNEKYSLIYSFQQKPQV", "aa_hexdigest": "3fd4d6a4509552848c764b9d90a2e8b3", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 438863, "stop": 439609, "observations": { "mutation": [ 438901, 439006, 439054, 439099 ], "stop": [ 438901, 439006, 439054, 439099 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAC9T0Q2", "query_cov": 1.0, "subject_cov": 0.6854838709677419, "identity": 0.9940000000000001, "score": 327.0, "evalue": 5.1e-112, "gene_start": 1, "gene_end": 170, "reference_start": 1, "reference_end": 170, "reference_sequence": "MKTINLKKEFNQYCISLKTQDLIKQYFNDEKLKEKLLKNRLIGLISLIIIFIIGVGILILGAFINKGNGANLNNGAITCVIIGIFFLLCLLIPYTIVANQNKKIMHKYIEYHFNEVNVGQLIKKISTFFQEFRLLTEGFLNFKQSNQVIDINYCNEKYSLIYSFQQKPQVWLIKSTNGNKISSKEWDLKGNYDLKIYELEKWNAGFSKFYLENNIESIAFQIESKLSTIINDFEELWIKGGGFLNEEY" }, "paralog": false, "identity": 0.967741935483871, "score": 469.0, "evalue": 1.1e-170, "description": "Internal stop codon at 438901, 439006, 439054, 439099. Nonsense mutation around 438901, 439006, 439054, 439099.", "psc": { "uniref90_id": "UniRef90_A0AAC9T0Q2", "product": "DUF3137 domain-containing protein", "uniref50_id": "UniRef50_A0AAC9T0Q2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAC9T0Q2", "UniRef:UniRef50_A0AAC9T0Q2" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAC9T0Q2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T0Q2" ], "product": "DUF3137 domain-containing protein" } }, "id": "NKIECMJPJD_748", "locus": "GCA000828735_00734" }, { "type": "cds", "sequence": "contig_1", "start": 439724, "stop": 439891, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCATTTATTAAAACGCCAAATAAAGATTTTGAATTAGTTGATAACCAAATTGTTTTATCAAGCGATTATAAAAAACGTTATCAACGATATTTTGCAAAAATTACAGGTAGTAGATTAGGTTCTATTTTAAATATTGGTGATTTTACTAATCCAGTTCGTACCTGA", "aa": "MPFIKTPNKDFELVDNQIVLSSDYKKRYQRYFAKITGSRLGSILNIGDFTNPVRT", "aa_hexdigest": "617f674ff9601dd519bd78d4d9e34afa", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6397.276400000001, "isoelectric_point": 9.773100852966305 }, "id": "NKIECMJPJD_749", "locus": "GCA000828735_00735" }, { "type": "cds", "sequence": "contig_1", "start": 439898, "stop": 440254, "strand": "+", "frame": 2, "gene": null, "product": "MPN551 family DNA-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0C5RMD4", "UniRef:UniRef90_A0A2C9DZ60" ], "nt": "ATGGTTAAAATTTATAATGAACCAATCGATCCAATATACGCTCAAGCCGGTGTTGTAATTGAACCAAAAATCAAAGATTATGTTGAGACTGTTTTAAAAGTTAAATATAAACAATACGAACCTGCGCAAATTGGTTATGATGTGTTTAAAGATAATCAAATTTTTGGTGGAATTCCGGATGGTGAACCAATTGATGAAAATGGTCAATTATTGTACCCAAATCAACCAATGTTAGAAATTAAAACAACATCTATTGATAGTTTTTCATTTAAAACAATTGATTATGTTCTTGTGCTACAAAAAGATGAACATAATCATCCGATTATAAAAAAGAAAGGCGATAAACGTGCAAAATGA", "aa": "MVKIYNEPIDPIYAQAGVVIEPKIKDYVETVLKVKYKQYEPAQIGYDVFKDNQIFGGIPDGEPIDENGQLLYPNQPMLEIKTTSIDSFSFKTIDYVLVLQKDEHNHPIIKKKGDKRAK", "aa_hexdigest": "92bc89bd88d61187953e66aed1ecbabe", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "both", "pseudogene": { "start": 439724, "stop": 440557, "observations": { "mutation": [ 439889, 440252, 440483, 440543 ], "stop": [ 439889, 440252, 440483, 440543 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A2C9DZ60", "query_cov": 1.0, "subject_cov": 0.4097222222222222, "identity": 1.0, "score": 241.0, "evalue": 1.87e-78, "gene_start": 1, "gene_end": 118, "reference_start": 59, "reference_end": 176, "reference_sequence": "MPFIKTPNKDFGLVDNQIVLSSDYKKRYQRYFAKITGSRLGSILNIGDFTNPVRTWAMMVKIYNEPIDPIYAQAGVVIEPKIKDYVETVLKVKYKQYEPAQIGYDVFKDNQIFGGIPDGEPIDENGQLLYPNQPMLEIKTTSIDSFSFKTIDYVLVLQKDEHNHPIIKKKGDKRAKWFNNDESEVIISDEYQLQLGLYCYLRKISKGIFAIAFLTSEDYINPQAFDINKNEIILVNYELDLNEFEKVISKAKTWYEEYIIKGISPKLTKEDLEWYKVWINKYETNNLY" }, "paralog": false, "identity": 0.9783393501805054, "score": 538.0, "evalue": 4.89e-198, "description": "Internal stop codon at 439889, 440252, 440483, 440543. Nonsense mutation around 439889, 440252, 440483, 440543.", "psc": { "uniref90_id": "UniRef90_A0A2C9DZ60", "product": "MPN551 family DNA-binding protein", "uniref50_id": "UniRef50_A0A0C5RMD4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A2C9DZ60", "UniRef:UniRef50_A0A0C5RMD4" ] }, "pscc": { "uniref50_id": "UniRef50_A0A0C5RMD4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0C5RMD4" ], "product": "YqaJ viral recombinase domain-containing protein" } }, "id": "NKIECMJPJD_750", "locus": "GCA000828735_00736" }, { "type": "cds", "sequence": "contig_1", "start": 440312, "stop": 440485, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTATTGTTATTTAAGAAAAATTAATAAAGGAATTTTTGCTATTGCTTTTTTAACAAGTGAAGATTATATTAATCCGCAAGCATTTGATATTAATAAAAATGAAATTATTTTAGTTAATTATGAGCTTGATTTAAATGAATTTGAAAAAGTTATTTCAAAAGCAAAAACATGA", "aa": "MYCYLRKINKGIFAIAFLTSEDYINPQAFDINKNEIILVNYELDLNEFEKVISKAKT", "aa_hexdigest": "d308c6d778a7e6125f11f7e048d6f676", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6706.689999999999, "isoelectric_point": 5.179817008972167 }, "id": "NKIECMJPJD_751", "locus": "GCA000828735_00737" }, { "type": "cds", "sequence": "contig_1", "start": 440568, "stop": 441740, "strand": "+", "frame": 3, "gene": "thiI", "product": "putative tRNA sulfurtransferase", "db_xrefs": [ "COG:COG0301", "COG:HJ", "EC:2.8.1.4", "GO:0000049", "GO:0002937", "GO:0004810", "GO:0005524", "GO:0005829", "GO:0009228", "GO:0009229", "GO:0052837", "GO:0140741", "RefSeq:WP_006689284.1", "SO:0001217", "UniParc:UPI00016C036A", "UniRef:UniRef100_UPI00016C036A", "UniRef:UniRef50_Q9PQ71", "UniRef:UniRef90_Q9PQ71" ], "nt": "ATGAAACCAATAATTTATATTAAATATGGGGAATTAACACTAAAAGGTAAAAATCGAGCACAATTTATAAAAGTTTTAGTGCACAATATCAAACAAATATTAATACAATATCAAAATTTAACATACTGTGTTGGTTATGATAATTTGAAGATTATCAATTTAGAAAATTATAATTTAAAACAAATTATTGATGATTTGAAAGAAGTTTATGGAATTGCTTTTATTTGTATTGCTTATCAAGTTGATAAAGAACTTAGTGAAATTCAATTAGCTTGTAAAGAATTTATAAATGATTATGAACAAACATTTAAAATTGAAGCCCGTCGTAGTGATAAAAGTTTTAAATACAATTCAATGGAAATTAAACAAATGTGCGCTGCTTATTTATTGCAAAATTCACCCCTTTTAAAAGTTGATGTTCATTGTCCACAACTATTAATTAATATTGAAATAAAATATGATTGTGCTATAGTTTATGGTCATAAAATTATGGGTGCTAAAGGATTGCCGGTAGGTATTAATGGTAAGGCCTTAGTGTTACTGTCTGGAGGAATCGATTCTCCTGTTGCTTCTCGTTTAATTATGAAACGAGGAATTAGTATTGATTTTATTACATTTATTACTCCGCCGCACACCTCACAAAAAGCTTTAGATAAAACAATTGCATTAGCAAAACAAATAACATTAGATAATAAATTAACAAAAGCGAATCTATATGTATGCAATTTCACTAAATTACAAGACGAAATTGCGCACATTTCTAAAGAATCTTATCGAATTACACTTATGCGTCGTTATTTTATGCGTATTGCTAAACGTTTAGCAATAAGTGTTAAAGCAAATGCTTTAGTAACTGGGGAGGCACTTGGACAAGTTGCTTCACAAACTTTAAATAGCATGCAAACAATCAGTAGTGTTTTAGATAATTTTTTAGTTTTAAGACCTTTAATAGCTTACGATAAAGAAGAAATAATTAGTTTAGCTAAACGGTTTAATACTTATGAATTATCGATTTTACCATACGATGATTCATGTAGCTTGTTTGCACCTAAGAATCCAACAACCAATCCTAATGTTCAAACAGCTGCTAAATTAGAAGAAGAATCATTAGTTTTAGATGCAATTTATGAATTAGTTTACACTAAAGAAATCACTAAAATTAATTTATCATAA", "aa": "MKPIIYIKYGELTLKGKNRAQFIKVLVHNIKQILIQYQNLTYCVGYDNLKIINLENYNLKQIIDDLKEVYGIAFICIAYQVDKELSEIQLACKEFINDYEQTFKIEARRSDKSFKYNSMEIKQMCAAYLLQNSPLLKVDVHCPQLLINIEIKYDCAIVYGHKIMGAKGLPVGINGKALVLLSGGIDSPVASRLIMKRGISIDFITFITPPHTSQKALDKTIALAKQITLDNKLTKANLYVCNFTKLQDEIAHISKESYRITLMRRYFMRIAKRLAISVKANALVTGEALGQVASQTLNSMQTISSVLDNFLVLRPLIAYDKEEIISLAKRFNTYELSILPYDDSCSLFAPKNPTTNPNVQTAAKLEEESLVLDAIYELVYTKEITKINLS", "aa_hexdigest": "51e5587e6d3d4bfaaa8a2131e1ec7934", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "ups": { "uniparc_id": "UPI00016C036A", "ncbi_nrp_id": "WP_006689284.1", "uniref100_id": "UniRef100_UPI00016C036A", "db_xrefs": [ "SO:0001217", "UniParc:UPI00016C036A", "RefSeq:WP_006689284.1", "UniRef:UniRef100_UPI00016C036A" ] }, "ips": { "uniref100_id": "UniRef100_UPI00016C036A", "uniref90_id": "UniRef90_Q9PQ71" }, "psc": { "uniref90_id": "UniRef90_Q9PQ71", "gene": "thiI", "product": "Probable tRNA sulfurtransferase", "ec_ids": [ "2.8.1.4" ], "uniref50_id": "UniRef50_Q9PQ71", "cog_id": "COG0301", "cog_category": "HJ", "go_ids": [ "GO:0005829", "GO:0005524", "GO:0004810", "GO:0000049", "GO:0140741", "GO:0009228", "GO:0009229", "GO:0052837", "GO:0002937" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQ71", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQ71" ], "product": "Probable tRNA sulfurtransferase" }, "genes": [ "thiI" ], "id": "NKIECMJPJD_752", "locus": "GCA000828735_00738" }, { "type": "cds", "sequence": "contig_1", "start": 441758, "stop": 442300, "strand": "+", "frame": 2, "gene": "nrnA", "product": "Bifunctional oligoribonuclease/PAP phosphatase NrnA", "db_xrefs": [ "COG:COG0618", "COG:FT", "EC:3.1.13.3", "EC:3.1.3.7", "KEGG:K06881", "SO:0001217", "UniRef:UniRef50_A0A084EYE9", "UniRef:UniRef90_A0AAC9T261" ], "nt": "ATGCAAAAAGATTTGTTAACAAAATTAATTGAACAAACATATGGTTTTAATAAAATCACTATTTTTGTTCATACAAATCCTGATTGTGATGCTTTAGGTTCTGCTTTCGCATTAGCACGAATTTTAAAATTAAATACTTTTGGAACAAGAGTTAAAATTGTTGGGGTTAACGCATTAAATCCTAATGATTTTAAAAATTTTTTTACATTTGATCCCAATGAAGTTGATGATGAATTTATTAAAGATTCAATAGCTTTTATTGTTGATACAGCTAATCAAGAAAGAGTTTTATCACAAAAACATAGATTAGCAAAAAAAACAATTTTAGTTGATCATCATGTAAAAACAATAACTTATACTGATCTTGAATACATTAATGATCAATCAATTGCAGCTTGTGAAATGATAGCATATTCATTAATGCATACTAATTTAAATTTTGATTTAAAAACTTTAAATTATCTTTTATTAGGTATTACTACTGATTCAAATCGTTTGATGTACGATAAAGTAAGTGATTTTACATATGAGATTATGTCTTGA", "aa": "MQKDLLTKLIEQTYGFNKITIFVHTNPDCDALGSAFALARILKLNTFGTRVKIVGVNALNPNDFKNFFTFDPNEVDDEFIKDSIAFIVDTANQERVLSQKHRLAKKTILVDHHVKTITYTDLEYINDQSIAACEMIAYSLMHTNLNFDLKTLNYLLLGITTDSNRLMYDKVSDFTYEIMS", "aa_hexdigest": "0e76b0b633066f5db39864654bb2ecd2", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "genes": [ "nrnA" ], "truncated": "3-prime", "pseudogene": { "start": 441758, "stop": 442603, "observations": { "mutation": [ 442298, 442442, 442469, 442517 ], "stop": [ 442298, 442442, 442469, 442517 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAC9T261", "query_cov": 1.0, "subject_cov": 0.5607476635514018, "identity": 1.0, "score": 357.0, "evalue": 8.91e-123, "gene_start": 1, "gene_end": 180, "reference_start": 1, "reference_end": 180, "reference_sequence": "MQKDLLTKLIEQTYGFNKITIFVHTNPDCDALGSAFALARILKLNTFGTRVKIVGVNALNPNDFKNFFTFDPNEVDDEFIKDSIAFIVDTANQERVLSQKHRLAKKTILVDHHVKTITYTDLEYINDQSIAACEMIAYSLMHTNLNFDLKTLNYLLLGITTDSNRLMYDKVSDFTYEIMSWFFKNNVKHYQIYQQLYERNLNDILFDNELVKTIKTQGQVAYLNIDPQWNQKYHFTRWGDKVYLLSNIKNYPIWFIVYFDENTNTYKVSLRSNKYKVRLVASQFNGGGHDLAAGCSLTNIDQLENLLSALDALIKNKIVVD" }, "paralog": false, "identity": 0.9857651245551602, "score": 551.0, "evalue": 8.8e-202, "description": "Internal stop codon at 442298, 442442, 442469, 442517. Nonsense mutation around 442298, 442442, 442469, 442517.", "psc": { "uniref90_id": "UniRef90_A0AAC9T261", "gene": "NrnA", "product": "Bifunctional oligoribonuclease/PAP phosphatase NrnA", "ec_ids": [ "3.1.13.3", "3.1.3.7" ], "uniref50_id": "UniRef50_A0A084EYE9", "cog_id": "COG0618", "cog_category": "FT", "kegg_orthology_id": "K06881", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAC9T261", "EC:3.1.13.3", "EC:3.1.3.7", "UniRef:UniRef50_A0A084EYE9", "COG:COG0618", "COG:FT", "KEGG:K06881" ] }, "pscc": { "uniref50_id": "UniRef50_A0A084EYE9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A084EYE9" ], "product": "Phosphoesterase DHH family protein" } }, "id": "NKIECMJPJD_753", "locus": "GCA000828735_00739" }, { "type": "cds", "sequence": "contig_1", "start": 442592, "stop": 442723, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02272.25" ], "nt": "TTGGTTGCTAGTCAATTTAATGGAGGCGGACATGATTTGGCAGCAGGTTGTAGTTTAACTAATATAGATCAATTAGAAAATTTATTAAGTGCTCTTGATGCACTAATTAAAAATAAAATAGTTGTTGATTAA", "aa": "MVASQFNGGGHDLAAGCSLTNIDQLENLLSALDALIKNKIVVD", "aa_hexdigest": "74e03cff854949803a508fe35bf5c2d5", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02272.25", "name": "DHHA1", "length": 42, "aa_cov": 0.8372093023255814, "hmm_cov": 1.0, "evalue": 5.799727447315347e-09, "score": 31.958206176757812, "start": 3, "stop": 38 } ], "seq_stats": { "molecular_weight": 4470.0438, "isoelectric_point": 4.504115104675294 }, "id": "NKIECMJPJD_754", "locus": "GCA000828735_00740" }, { "type": "cds", "sequence": "contig_1", "start": 442808, "stop": 443020, "strand": "+", "frame": 2, "gene": null, "product": "putative protein UU416", "db_xrefs": [ "RefSeq:WP_006688799.1", "SO:0001217", "UniParc:UPI0000139799", "UniRef:UniRef100_Q9PQ73", "UniRef:UniRef50_Q9PQ73", "UniRef:UniRef90_Q9PQ73" ], "nt": "ATGAGTGATAAAAAAAGTGATTTTGAAAAGTTATTTGAGCGTTCAAAAGTACAAGAAACATCTATTTTCGATATTAATGATATTGATGAACAAAAACCGTACGAAGAACATTCGATTGAAGAATTATCATATATCTTAGAATATGAAAAAATTGATAAGAAAACACGTGAAAAGATCAAAAAAATTATTAAAGAGAAACAACAAAATTTATAA", "aa": "MSDKKSDFEKLFERSKVQETSIFDINDIDEQKPYEEHSIEELSYILEYEKIDKKTREKIKKIIKEKQQNL", "aa_hexdigest": "e4bbf817419d2e96c3bdfe34fbf7e781", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "ups": { "uniparc_id": "UPI0000139799", "ncbi_nrp_id": "WP_006688799.1", "uniref100_id": "UniRef100_Q9PQ73", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139799", "RefSeq:WP_006688799.1", "UniRef:UniRef100_Q9PQ73" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQ73", "uniref90_id": "UniRef90_Q9PQ73" }, "psc": { "uniref90_id": "UniRef90_Q9PQ73", "product": "Uncharacterized protein UU416", "uniref50_id": "UniRef50_Q9PQ73" }, "pscc": { "uniref50_id": "UniRef50_Q9PQ73", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQ73" ], "product": "Uncharacterized protein UU416" }, "genes": [], "id": "NKIECMJPJD_755", "locus": "GCA000828735_00741" }, { "type": "cds", "sequence": "contig_1", "start": 443020, "stop": 443286, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTATTAATTTAAATGTGCATTCACATTATTCTTTACTTAATAGTACTTTAAGTATTGATGATTTAATTAAATACGCATTAGATAACAAACAACCATACGTTTGCTTAACTGATTTAAATAATATGTATGGCTGTATCGAATTTTATGATAAAGCAAAAGCACATAATTTAACTCCTATTATCGGATTAGAATTTGAATATCAAAATGCTACTTTAGTAGCTTATGCTAAAAATTATAATGGCTATTTAAAATTAATCAAATGA", "aa": "MFINLNVHSHYSLLNSTLSIDDLIKYALDNKQPYVCLTDLNNMYGCIEFYDKAKAHNLTPIIGLEFEYQNATLVAYAKNYNGYLKLIK", "aa_hexdigest": "7af14f2924425a8c85c716e7c2011026", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10170.582199999995, "isoelectric_point": 6.2359422683715815 }, "id": "NKIECMJPJD_756", "locus": "GCA000828735_00742" }, { "type": "cds", "sequence": "contig_1", "start": 443299, "stop": 444774, "strand": "+", "frame": 1, "gene": "dnaE", "product": "DNA polymerase III subunit alpha", "db_xrefs": [ "COG:COG0587", "COG:L", "EC:2.7.7.7", "GO:0003887", "GO:0005737", "GO:0006260", "GO:0008408", "SO:0001217", "UniRef:UniRef50_Q9PQ74", "UniRef:UniRef90_Q9PQ74" ], "nt": "ATGACAAGTAAAGAATTTGAAATTCAAGATGATTTTGATGACTTAATTATTGTTTGTAAAAAAGGAACTATAGTTTTTAAGAGTCCTAATTTTTATCAAACGCATGATCAAAATGCTCCTAATGCGATTGCATTACAAAGTGTTTTCTATGCTAACAAAAATGATAAAATTGTTTTTTTAGCAATGTTAGCAATCAAAAATGATTTAAAGTTAGAAGATTTTAAAAATTGTTGCGATTTTGATAATAATCATTTTCTAAATGATAATCTAGCACAATCATTTTTTTCACCAATTGCTTTAAATAATTTAAATAAAGTTTTAAATGAATTAAAAGTACAAATTCATGATTTACCAATTAATATTCCGGTTTATGATAAACAAAATTCAATTATTTCAAGTGAAATTTTAAAACAATTATGTATTAGCGGATTAAAAAAACGCTTAAATGCTAACGATGGCCAAGTTAATAAAATTTATGTTCAAAGATTAAAATATGAATTAGATGTTATTAATGAAAAACAATTTGATGATTATTTTTTAATTGTTTATGATTTTATTAATTTTGCTAAAAGTAATGGTATCATTGTAGGGCCAGGACGTGGGAGTGCTGCTGGTTCGTTAGTAGCTTATTGTTTACATATTACAGATATTGATCCTATTAAACATAATTTAATTTTTGAACGTTTTTTAAATCCTACCAGAAAAAGTATGCCAGATATTGATACTGATATTATGGATGAAAAACGCGATCTAGTTATTGAATATTTATTTGAAAAATATGGCAATGATCATGTTGCTTACATTTTAACGTTTCAACGTTTAAAAGCGAAGATGGCTATTCGAGATGTAGGGCGGATTTTGGGAATTGATTTAAAAGTAATTGATAAAATTTGTAAAAATATTAAACCTGAATATGATGAAGATCTTGATTTAGCAATTAAAAAAAATACGATTTTAAAAGAAATGTATCTTTTGCATAAAGAGTTGTTTGAAATTTCTAAAAAATTAATTAATGCTCCTCGCCAAATAGGCACACACGCTGCAGGGATAATTTTAAGTGATAGTTTAATTTCAAATATTATTCCAATTCAACTAGGTATTAATGACCGTCCTTTATCACAATATTCAATGGAATATTTAGAGCGTTTTGGTTTAATTAAAATGGATTTATTAGGTTTGAAAAATTTAACAATTATTGATAATGTTTTAAAAATGATTTATGAAAATCAAAATAAAAAAATTGATCTCTTTAACATTAACTATAATGATAAATTTGTCTTTGAAGATTTAGCTAAAGCAAGGACAAACGGAATATTTCAACTAGAATCGCCCGGAATGAAAAAAGTACTATTAAAGGTCAAACCTCAGAATATTGAAGATATTTCTATTGTTTCAGCTCTTTTTCGTCCTGGTCCACAACAAAATATTAAAACATTTGTTGAACGTCGTTTTAAACGTGAAGAGTTTAGTTATTGA", "aa": "MTSKEFEIQDDFDDLIIVCKKGTIVFKSPNFYQTHDQNAPNAIALQSVFYANKNDKIVFLAMLAIKNDLKLEDFKNCCDFDNNHFLNDNLAQSFFSPIALNNLNKVLNELKVQIHDLPINIPVYDKQNSIISSEILKQLCISGLKKRLNANDGQVNKIYVQRLKYELDVINEKQFDDYFLIVYDFINFAKSNGIIVGPGRGSAAGSLVAYCLHITDIDPIKHNLIFERFLNPTRKSMPDIDTDIMDEKRDLVIEYLFEKYGNDHVAYILTFQRLKAKMAIRDVGRILGIDLKVIDKICKNIKPEYDEDLDLAIKKNTILKEMYLLHKELFEISKKLINAPRQIGTHAAGIILSDSLISNIIPIQLGINDRPLSQYSMEYLERFGLIKMDLLGLKNLTIIDNVLKMIYENQNKKIDLFNINYNDKFVFEDLAKARTNGIFQLESPGMKKVLLKVKPQNIEDISIVSALFRPGPQQNIKTFVERRFKREEFSY", "aa_hexdigest": "452ecd69a33f5fe94cf8030b56c9892b", "start_type": "ATG", "rbs_motif": null, "genes": [ "dnaE" ], "truncated": "both", "pseudogene": { "start": 443020, "stop": 445077, "observations": { "mutation": [ 443284, 443293, 444772 ], "stop": [ 443284, 443293, 444772 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQ74", "query_cov": 1.0, "subject_cov": 0.5067079463364293, "identity": 1.0, "score": 968.0, "evalue": 0.0, "gene_start": 1, "gene_end": 491, "reference_start": 94, "reference_end": 584, "reference_sequence": "MFINLNVHSHYSLLNSTLSIDDLIKYALDNKQPYVCLTDLNNMYGCIEFYDKAKAHNLTPIIGLEFEYQNATLVAYAKNYNGYLKLIKWSSWIMTSKEFEIQDDFDDLIIVCKKGTIVFKSPNFYQTHDQNAPNAIALQSVFYANKNDKIVFLAMLAIKNDLKLEDFKNCCDFDNNHFLNDNLAQSFFSPIALNNLNKVLNELKVQIHDLPINIPVYDKQNSIISSEILKQLCISGLKKRLNANDGQVNKIYVQRLKYELDVINEKQFDDYFLIVYDFINFAKSNGIIVGPGRGSAAGSLVAYCLHITDIDPIKHNLIFERFLNPTRKSMPDIDTDIMDEKRDLVIEYLFEKYGNDHVAYILTFQRLKAKMAIRDVGRILGIDLKVIDKICKNIKPEYDEDLDLAIKKNTILKEMYLLHKELFEISKKLINAPRQIGTHAAGIILSDSLISNIIPIQLGINDRPLSQYSMEYLERFGLIKMDLLGLKNLTIIDNVLKMIYENQNKKIDLFNINYNDKFVFEDLAKARTNGIFQLESPGMKKVLLKVKPQNIEDISIVSALFRPGPQQNIKTFVERRFKREEFSYWNEATRKILEPTYGIIVYQEQVIELVKTIANFDIATSDNFRRAISKKDEKILIQLKDDFINGALNNNYKQPLVNQIFEYIFSFAHYGFNHSHSLAYSYISYWLAYLKHYYTLEFLSVLLSHTSASKDKLLSYLNEAKEFNISIKGPDIQYFSNDFVIDTQKQIIRFGFKTIKGFGDELLKKIKSALQKSTLSDYISYIDALKKGNVSLKNIEILIRVGTFDSFGINRLFLLNNLNEIFEKTGLNGHFFDLNLVGLDYAKDMSVNDRYLDDEIQYLGIDLNSLNYANYKTEIDYNKLKYTIKDFYEINTNYETNILAQVLNIKQSKTKNGNDIFYLETLIDNKKQTLTIFQNSKYLIDEISIGGIYVFGVKLLNHFNFIVNIKQRI" }, "paralog": false, "identity": 0.9956204379562044, "score": 1353.0, "evalue": 0.0, "description": "Internal stop codon at 443284, 443293, 444772. Nonsense mutation around 443284, 443293, 444772.", "psc": { "uniref90_id": "UniRef90_Q9PQ74", "gene": "dnaE", "product": "DNA polymerase III subunit alpha", "ec_ids": [ "2.7.7.7" ], "uniref50_id": "UniRef50_Q9PQ74", "cog_id": "COG0587", "cog_category": "L", "go_ids": [ "GO:0005737", "GO:0008408", "GO:0003887", "GO:0006260" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQ74", "EC:2.7.7.7", "UniRef:UniRef50_Q9PQ74", "COG:COG0587", "COG:L", "GO:0005737", "GO:0008408", "GO:0003887", "GO:0006260" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQ74", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQ74" ], "product": "DNA polymerase III subunit alpha" } }, "id": "NKIECMJPJD_757", "locus": "GCA000828735_00743" }, { "type": "cds", "sequence": "contig_1", "start": 444835, "stop": 445077, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF17657.6" ], "nt": "GTGATTGAATTAGTTAAAACTATTGCAAATTTTGATATTGCTACTTCCGATAATTTTCGCCGTGCAATATCAAAAAAAGATGAAAAAATTTTAATCCAATTAAAAGATGATTTTATCAATGGTGCTTTGAATAATAATTATAAACAACCATTGGTTAATCAAATTTTTGAATATATTTTTTCGTTTGCACATTATGGTTTTAACCATTCACATTCACTAGCATATTCATATATTAGTTATTGA", "aa": "MIELVKTIANFDIATSDNFRRAISKKDEKILIQLKDDFINGALNNNYKQPLVNQIFEYIFSFAHYGFNHSHSLAYSYISY", "aa_hexdigest": "def5f52f07645ad8ece49ffa233e6fdc", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF17657.6", "name": "DNA_pol3_finger", "length": 45, "aa_cov": 0.5625, "hmm_cov": 1.0, "evalue": 4.804748772715431e-09, "score": 31.325239181518555, "start": 2, "stop": 46 } ], "seq_stats": { "molecular_weight": 9369.495299999999, "isoelectric_point": 6.816268348693847 }, "id": "NKIECMJPJD_758", "locus": "GCA000828735_00744" }, { "type": "cds", "sequence": "contig_1", "start": 445411, "stop": 445929, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATACGAGTCGGCACTTTTGATAGTTTTGGAATTAATCGTTTATTTTTATTAAATAATTTGAATGAAATTTTTGAAAAAACAGGATTGAATGGTCATTTTTTTGATTTAAATTTAGTGGGTTTAGATTATGCAAAAGATATGAGTGTTAATGATCGTTATTTAGATGATGAAATTCAGTATTTAGGAATTGATTTGAATAGTTTAAATTACGCAAATTATAAAACTGAAATTGATTATAATAAACTAAAATATACAATTAAAGACTTTTATGAAATAAACACAAATTATGAAACAAACATTCTTGCACAAGTTTTAAACATTAAGCAATCAAAAACTAAAAACGGGAATGATATCTTTTATTTAGAAACACTAATTGATAATAAAAAACAAACACTAACAATTTTTCAAAATAGTAAATATTTAATTGATGAAATTAGTATTGGAGGTATTTATGTTTTTGGAGTTAAATTATTAAATCATTTTAATTTTATTGTAAATATTAAGCAAAGGATTTAG", "aa": "MIRVGTFDSFGINRLFLLNNLNEIFEKTGLNGHFFDLNLVGLDYAKDMSVNDRYLDDEIQYLGIDLNSLNYANYKTEIDYNKLKYTIKDFYEINTNYETNILAQVLNIKQSKTKNGNDIFYLETLIDNKKQTLTIFQNSKYLIDEISIGGIYVFGVKLLNHFNFIVNIKQRI", "aa_hexdigest": "8ec47a197fdc19faae0158f3e29a79a7", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 20156.713600000006, "isoelectric_point": 5.382845878601075 }, "id": "NKIECMJPJD_759", "locus": "GCA000828735_00745" }, { "type": "cds", "sequence": "contig_1", "start": 446029, "stop": 446802, "strand": "+", "frame": 1, "gene": "polA", "product": "5'-3' exonuclease", "db_xrefs": [ "COG:COG0258", "COG:L", "EC:3.1.11.-", "GO:0003677", "GO:0008409", "GO:0017108", "GO:0033567", "SO:0001217", "UniRef:UniRef50_Q9PQ75", "UniRef:UniRef90_Q9PQ75" ], "nt": "ATGCCTACTAATGCCATTAAATTAGTAGCCTCAATGATTTTTAAGATTTTAAATCAAGAACAATTTACTTATGGTTTAATTGCTATGGACGCTTCTAAACAAACTTTTCGTGCTCAAGAATATGCTGCTTACAAAGCGACACGTAAACCTATGGATGCAAAACTAATTGTTCAATTACCCTATTTAAAAAAACTATTCGTTGCTATGGGTTTTCGAATAATTTCACAACCTGGAATTGAAGCTGACGATTTTGTTGGTTCTTTTAGTAATTTAATGAATAAGTTTGATGTTGATACAACTATTTATAGTTCAGATCGTGATATGTTGCAATTAATTAACAAAAACACAAAACTTAAACTCTTAAAAACAGGGACAAGTGTTATGCAAGAAATTAATTTAACAAATTTTTCATTATTAAATAATGGCATTTTACCGTATCAAATTATTGAATATAAGGGTTTAGTGGGTGATAGTAGTGATAATTTAATTGGCGTGAAGGGAATCGGCCCTAAAACAGCTATTAATTTACTTTTAAAATATGATAATATAGAAAATATTTATACTAATTTAGAAAAAATAGCACCTAGTGTTAAAAATAAATTAATTGAACACAAAGATATGGCTTTTTTATCAAAAAAATTAGCGGCGATTCAAACGGATTTATTGTTTGATGAAAAATTAGAAAATTTTGTTTTAAAACCATATAATACTCAAGAATTAGATGAGTTATTCACATCTTTAAAAATAAATAATATGCAAAATTATTATAAATAA", "aa": "MPTNAIKLVASMIFKILNQEQFTYGLIAMDASKQTFRAQEYAAYKATRKPMDAKLIVQLPYLKKLFVAMGFRIISQPGIEADDFVGSFSNLMNKFDVDTTIYSSDRDMLQLINKNTKLKLLKTGTSVMQEINLTNFSLLNNGILPYQIIEYKGLVGDSSDNLIGVKGIGPKTAINLLLKYDNIENIYTNLEKIAPSVKNKLIEHKDMAFLSKKLAAIQTDLLFDEKLENFVLKPYNTQELDELFTSLKINNMQNYYK", "aa_hexdigest": "d55ccb62a745d43abc2057cfec32771f", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "psc": { "uniref90_id": "UniRef90_Q9PQ75", "query_cov": 1.0, "subject_cov": 0.889273356401384, "identity": 1.0, "score": 498.0, "evalue": 1.7e-177, "valid": true, "gene": "polA", "product": "5'-3' exonuclease", "ec_ids": [ "3.1.11.-" ], "uniref50_id": "UniRef50_Q9PQ75", "cog_id": "COG0258", "cog_category": "L", "go_ids": [ "GO:0008409", "GO:0017108", "GO:0003677", "GO:0033567" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQ75", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQ75" ], "product": "5'-3' exonuclease" }, "genes": [ "polA" ], "id": "NKIECMJPJD_760", "locus": "GCA000828735_00746" }, { "type": "cds", "sequence": "contig_1", "start": 446844, "stop": 447110, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01149.29" ], "nt": "ATGCCAGAGCTACCAGAAGTACAAACGATTGTTGATTATTTAAATCATCATGTTCTCGATATTTTTATTAAAAAAACTATTGTTCATTTACCTAAAATTTTAAAAAATAAAACACCACAAGAATTTGAAAAACTGTTAATTAATCATAAAATAGTCAAAATTAAACGTCTTGGTAAATATTTATTATTTTTTTTAAGTAATAATTTAGTATTAAGTGTACATTTGCGGATGGAGGGAAAATTTTATTATCAAGCAAAAGAGGAATGA", "aa": "MPELPEVQTIVDYLNHHVLDIFIKKTIVHLPKILKNKTPQEFEKLLINHKIVKIKRLGKYLLFFLSNNLVLSVHLRMEGKFYYQAKEE", "aa_hexdigest": "7156524dd7718dbd48a55d37088c74e6", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01149.29", "name": "Fapy_DNA_glyco", "length": 87, "aa_cov": 0.9772727272727273, "hmm_cov": 1.0, "evalue": 2.244315812229648e-25, "score": 85.1330337524414, "start": 1, "stop": 86 } ], "seq_stats": { "molecular_weight": 10528.469799999993, "isoelectric_point": 9.522576713562014 }, "id": "NKIECMJPJD_761", "locus": "GCA000828735_00747" }, { "type": "cds", "sequence": "contig_1", "start": 447156, "stop": 447671, "strand": "+", "frame": 3, "gene": "mutM", "product": "DNA-formamidopyrimidine glycosylase", "db_xrefs": [ "COG:COG0266", "COG:L", "EC:3.2.2.23", "GO:0003684", "GO:0005737", "GO:0006281", "GO:0006284", "GO:0008270", "GO:0008534", "GO:0034039", "GO:0046872", "GO:0140078", "KEGG:K10563", "SO:0001217", "UniRef:UniRef50_Q9PQ76", "UniRef:UniRef90_Q9PQ76" ], "nt": "ATGCAATTAAGATATAATGATACGCGTCAATTTGGAACTTTTCACATTTATGAGCAACAATCTTTTTTAGATTCAAAAGAATTAAAAAAGATTGCTTTAGATCCGCTAGATAATAATTTTTCAGCGCAGTATTTGTATGAAAAATTAAAAAAAAGTAATAAAGCCATTAAAACAGCCTTGTTAGATCAATCTGTTGTTTCAGGAATTGGTAACATTTATGCTGATGAAATTTTATTTGCAGCTAAAATTTTTCCTACAATATTAGCTAAAAATCTTACTTTAAAAAATTATGAAAAGATCACAAAAGAAGCACAACGAATCTTATTATTATCAATTAAAAATAAAGGAACAACAATCCACACTTATAAATTTGGTAATGATGAAACAGGTTTGTTTCAAAAGATGTTATTAGTTCACACACACGCTAAAGAACCATGCCAAATTTGTGGAACAATTATTCAAAAAACAAAGGTCAACGGTCGGGGAACTTATTATTGTCCAAATTGTCAAAACTAA", "aa": "MQLRYNDTRQFGTFHIYEQQSFLDSKELKKIALDPLDNNFSAQYLYEKLKKSNKAIKTALLDQSVVSGIGNIYADEILFAAKIFPTILAKNLTLKNYEKITKEAQRILLLSIKNKGTTIHTYKFGNDETGLFQKMLLVHTHAKEPCQICGTIIQKTKVNGRGTYYCPNCQN", "aa_hexdigest": "51d8ee29228632c88ad19a7c586fe634", "start_type": "ATG", "rbs_motif": null, "genes": [ "mutM" ], "truncated": "both", "pseudogene": { "start": 446856, "stop": 447677, "observations": { "mutation": [ 447108, 447669 ], "stop": [ 447108, 447669 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQ76", "query_cov": 1.0, "subject_cov": 0.6173285198555957, "identity": 0.953, "score": 327.0, "evalue": 1.44e-111, "gene_start": 1, "gene_end": 171, "reference_start": 105, "reference_end": 275, "reference_sequence": "MPELPEVQTIVDYLNHHVLDIFIKKTIVHLPKILKNKTAQEFENLLINHKIVKIERLGKYLLFFLSNNLVLCVHLRMEGKFYYQAKDEWFNLAHTHIIIEFNNGMQLRYNDTRQFGTFHIYEQESFLDSKELKKIALDPLDNNFSAQYLYGKLKKSNKAIKTALLDQSNVSGIGNIYADEILFAAKIFPTILAKNLTLKDYEKIAKEARRILLLSIENKGTTIHTYKFVNDETGLFQKMLLVHTHAKEPCQICGTIIQKTKVNGRGTYYCPNCQNQN" }, "paralog": false, "identity": 0.945054945054945, "score": 518.0, "evalue": 3.09e-189, "description": "Internal stop codon at 447108, 447669. Nonsense mutation around 447108, 447669.", "psc": { "uniref90_id": "UniRef90_Q9PQ76", "gene": "mutM", "product": "DNA-formamidopyrimidine glycosylase", "ec_ids": [ "3.2.2.23" ], "uniref50_id": "UniRef50_Q9PQ76", "cog_id": "COG0266", "cog_category": "L", "kegg_orthology_id": "K10563", "go_ids": [ "GO:0003684", "GO:0005737", "GO:0006281", "GO:0006284", "GO:0008270", "GO:0008534", "GO:0034039", "GO:0046872", "GO:0140078" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQ76", "EC:3.2.2.23", "UniRef:UniRef50_Q9PQ76", "COG:COG0266", "COG:L", "KEGG:K10563", "GO:0003684", "GO:0005737", "GO:0006281", "GO:0006284", "GO:0008270", "GO:0008534", "GO:0034039", "GO:0046872", "GO:0140078" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQ76", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQ76" ], "product": "Formamidopyrimidine-DNA glycosylase" } }, "id": "NKIECMJPJD_762", "locus": "GCA000828735_00748" }, { "type": "tRNA", "sequence": "contig_1", "start": 447735, "stop": 447816, "strand": "+", "gene": "trnL", "product": "tRNA-Leu(caa)", "amino_acid": "Leu", "anti_codon": "caa", "score": 66.2, "nt": "GCTCAAGTGGCGAAATGGCAGACGCAGTTGACTCAAAATCAACCGAGAAATCATAAGGGTTCGAGTCCCTTCTTGAGCACCA", "db_xrefs": [ "SO:0000264" ], "anti_codon_pos": [ 447738, 447740 ], "id": "NKIECMJPJD_20", "locus": "GCA000828735_00749" }, { "type": "cds", "sequence": "contig_1", "start": 447985, "stop": 448104, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGCAATGTTTGTTAATACATTCAAGACTAATTTGGTTGATGAAAAGAAAATTTTTGAAGCAATTAATAAAAGTTTTAATTTTGATATCAAAACGTTTATTAATGATTTAGAATTGTGA", "aa": "MAMFVNTFKTNLVDEKKIFEAINKSFNFDIKTFINDLEL", "aa_hexdigest": "114efbf6cc858f86d42246b54e502a16", "start_type": "GTG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4630.3387999999995, "isoelectric_point": 4.992987251281736 }, "id": "NKIECMJPJD_763", "locus": "GCA000828735_00750" }, { "type": "cds", "sequence": "contig_1", "start": 448186, "stop": 448620, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02773.21", "PFAM:PF02772.21" ], "nt": "ATGCCATTAACAACAGTGTTAGCTCATGAATTACTAAAAGAAATCGAAAAACAAAGACGTAATAAAGAATTTGTTAAAATTCAAGCTGATATGAAATCACAAGTAAGTATTGATTATTCTAATTGTACGCCTGTTATTGAGACAATGTTGGTAAGTATTCAACATGATGAAGATTATAACATTGAATATTTTAATAAAAAAGTTAGCACAATTATGGATCAAATTGCTATAAAATATGGTTTAAATACCGATTTTAAAAAAATTATTAATTCCTCAGGGAGATTTGTAATTGGTGGTCCAATTGGTGACACTGGTTTAACTGGAAGAAAAATTATCGTAGATACTTATGGAGGAATAGGTCATCATGGTGGTGGTGCTTTTAGTGGCAAAGACCCTACTAAAGTTGATCGTAGTGCTTCATACTTTGCACGTTGA", "aa": "MPLTTVLAHELLKEIEKQRRNKEFVKIQADMKSQVSIDYSNCTPVIETMLVSIQHDEDYNIEYFNKKVSTIMDQIAIKYGLNTDFKKIINSSGRFVIGGPIGDTGLTGRKIIVDTYGGIGHHGGGAFSGKDPTKVDRSASYFAR", "aa_hexdigest": "97b25a522ab36d4664c191a65a84dd5b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02773.21", "name": "S-AdoMet_synt_C", "length": 48, "aa_cov": 0.3333333333333333, "hmm_cov": 1.0, "evalue": 4.884255718481181e-26, "score": 86.8976821899414, "start": 97, "stop": 144 }, { "id": "PF02772.21", "name": "S-AdoMet_synt_M", "length": 99, "aa_cov": 0.6597222222222222, "hmm_cov": 1.0, "evalue": 4.157189663846437e-19, "score": 64.4543228149414, "start": 1, "stop": 95 } ], "seq_stats": { "molecular_weight": 16030.131299999983, "isoelectric_point": 8.609317588806153 }, "id": "NKIECMJPJD_764", "locus": "GCA000828735_00751" }, { "type": "cds", "sequence": "contig_1", "start": 448792, "stop": 449028, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00438.25" ], "nt": "ATGGTAGAAAGAAAAGAATTTAGTATGAAATATAAAAAAATTATAACGAGCGAATCTGTTGGTGCAGGCCATCCAGATAAAATATGTGATCAGATTAGTGATGCAATTTTAGATGAATGCTTAGCACAAGATAAAAATTCACGTGTAGCTTGTGAAGTTTTAGCTTGTAATCGTTTAATTGTTATTGCTGGGGAAATTACCACGCATGCATATGTTGATGTAGTAAAAACCGCTTGA", "aa": "MVERKEFSMKYKKIITSESVGAGHPDKICDQISDAILDECLAQDKNSRVACEVLACNRLIVIAGEITTHAYVDVVKTA", "aa_hexdigest": "c838d2f0fb37490525d36f3ca0b3810f", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0035AE390E", "query_cov": 0.8333333333333334, "subject_cov": 0.8441558441558441, "identity": 0.631, "score": 80.9, "evalue": 1.51e-18, "valid": false, "product": "S-adenosylmethionine synthetase N-terminal domain-containing protein", "ec_ids": [ "2.5.1.6" ], "uniref50_id": "UniRef50_A0A355EM24", "kegg_orthology_id": "K00789" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00438.25", "name": "S-AdoMet_synt_N", "length": 65, "aa_cov": 0.8333333333333334, "hmm_cov": 1.0, "evalue": 2.4841345836442082e-30, "score": 100.57001495361328, "start": 13, "stop": 77 } ], "seq_stats": { "molecular_weight": 8583.846499999996, "isoelectric_point": 5.574563789367674 }, "id": "NKIECMJPJD_765", "locus": "GCA000828735_00752" }, { "type": "cds", "sequence": "contig_1", "start": 449028, "stop": 449117, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTTATACAGGTGATAATGCTGCTATGATTGGGGCTTATGCTTATGCTTTAATTAAAAATCATAAGAAATCAATATTAATAAAATAA", "aa": "MIYTGDNAAMIGAYAYALIKNHKKSILIK", "aa_hexdigest": "efc1a6022e29d67b96240f954ef4ec30", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3212.8245000000006, "isoelectric_point": 9.602904319763184 }, "id": "NKIECMJPJD_766", "locus": "GCA000828735_00753" }, { "type": "cds", "sequence": "contig_1", "start": 449460, "stop": 449990, "strand": "-", "frame": 1, "gene": "tsaD", "product": "tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD", "db_xrefs": [ "COG:COG0533", "COG:J", "EC:2.3.1.234", "GO:0002949", "GO:0005506", "GO:0005737", "GO:0061711", "SO:0001217", "UniRef:UniRef50_Q9PQ78", "UniRef:UniRef90_Q9PQ78" ], "nt": "ATGAATGATAATTATTTAATTTTAAGCATTGAATCAAGTTGTGATGAAACAAGTTTAGCGTTATTTGAAAATAATAAATTAATTGCTCATAAAATTAGTAGTTCAGCATCTATACAATCACTTCATGGCGGTGTTGTTCCTGAATTAGCAAGTCGTTATCATGAACAAAATATTAATCACCTATTTAATGAAATTTTAAATGAAACAAAAATCAACCCTTTAACTATCACTCACGTTGCTTACACTGCAATGCCTGGTTTGCCAGGTTGTTTACATGTAGGAAAAGTTTTTGCGAAACAATTAGCTGTTTTAATTAATGCTGAATTAGTGCCAATCAATCATTTACATGCGCATGTTTTTAGTGCAAGTATTAATCAAAATTTGACTTTTCCATTTTTGGGATTAGTGGTTTCAGGAGGGGAAAGTTGTATATATTTAGTAAATGATTATGATGAAATCAAAGTTTTAAATCAAACACACGATGATGCAATTGGTGAATGTTATGATAAAATTGCTCGTGTTCTTGGTTGA", "aa": "MNDNYLILSIESSCDETSLALFENNKLIAHKISSSASIQSLHGGVVPELASRYHEQNINHLFNEILNETKINPLTITHVAYTAMPGLPGCLHVGKVFAKQLAVLINAELVPINHLHAHVFSASINQNLTFPFLGLVVSGGESCIYLVNDYDEIKVLNQTHDDAIGECYDKIARVLG", "aa_hexdigest": "a58b58c071320208a689d5b98d05db4a", "start_type": "ATG", "rbs_motif": null, "genes": [ "tsaD" ], "truncated": "5-prime", "pseudogene": { "start": 449157, "stop": 449990, "observations": { "mutation": [ 449462 ], "stop": [ 449462 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQ78", "query_cov": 1.0, "subject_cov": 0.55, "identity": 1.0, "score": 353.0, "evalue": 4.89e-121, "gene_start": 1, "gene_end": 176, "reference_start": 1, "reference_end": 176, "reference_sequence": "MNDNYLILSIESSCDETSLALFENNKLIAHKISSSASIQSLHGGVVPELASRYHEQNINHLFNEILNETKINPLTITHVAYTAMPGLPGCLHVGKVFAKQLAVLINAELVPINHLHAHVFSASINQNLTFPFLGLVVSGGESCIYLVNDYDEIKVLNQTHDDAIGECYDKIARVLGWKYPGGPIIDKNYQENLATLEFIKSQPAAKDFSFSGLKTAVINYIHNAKQKKISFDPVVVASSFQKFAINEIIKKIKYYLNLYKLNHLAIGGGVSANSLLRKKIQSLDVISYIPEMIYTGDNAAMIGAYAYALIKNHKKSILIK" }, "paralog": false, "identity": 0.9963898916967509, "score": 548.0, "evalue": 1.2e-200, "description": "Internal stop codon at 449462. Nonsense mutation around 449462.", "psc": { "uniref90_id": "UniRef90_Q9PQ78", "gene": "tsaD", "product": "tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD", "ec_ids": [ "2.3.1.234" ], "uniref50_id": "UniRef50_Q9PQ78", "cog_id": "COG0533", "cog_category": "J", "go_ids": [ "GO:0002949", "GO:0005506", "GO:0005737", "GO:0061711" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQ78", "EC:2.3.1.234", "UniRef:UniRef50_Q9PQ78", "COG:COG0533", "COG:J", "GO:0002949", "GO:0005506", "GO:0005737", "GO:0061711" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQ78", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQ78" ], "product": "tRNA N6-adenosine threonylcarbamoyltransferase" } }, "id": "NKIECMJPJD_767", "locus": "GCA000828735_00754" }, { "type": "cds", "sequence": "contig_1", "start": 450840, "stop": 450944, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00133.28" ], "nt": "ATGGTTCTAGATGGTGAAGGATATAAGATGTCAAAATCAAAAGGAAATGTTGTTGATCCATTAGATGTATGTAAGATTTATGGAGCAGATGTTTTAAGATTATGA", "aa": "MVLDGEGYKMSKSKGNVVDPLDVCKIYGADVLRL", "aa_hexdigest": "d98753bcee388e2b6d0da9490fd3d33f", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00133.28", "name": "tRNA-synt_1", "length": 34, "aa_cov": 1.0, "hmm_cov": 1.0, "evalue": 1.2176721714470994e-12, "score": 42.81079864501953, "start": 1, "stop": 34 } ], "seq_stats": { "molecular_weight": 3714.333599999999, "isoelectric_point": 5.952997779846191 }, "id": "NKIECMJPJD_768", "locus": "GCA000828735_00755" }, { "type": "cds", "sequence": "contig_1", "start": 451206, "stop": 451298, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTTTTGATGAGAATCATAATGCAATTATGGATCATGAATTAGTAGAAAATATCATTAATGTTTTAAATGAAAAAGGTGTTAATGCATGA", "aa": "MIFDENHNAIMDHELVENIINVLNEKGVNA", "aa_hexdigest": "266c18d9a7b233b9d2655185bf5e2e3e", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3436.8241000000007, "isoelectric_point": 4.346102714538575 }, "id": "NKIECMJPJD_769", "locus": "GCA000828735_00756" }, { "type": "cds", "sequence": "contig_1", "start": 451338, "stop": 451481, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATCGTGCCACAAAACAATGGTTTGTTTCTATTGAAAAAATTTTACCTAACATTTTGCAGACCCTAAATAACGATGTTAAATCAACTAGTTTTAGAGGAATTGAAAGAATGCATGAAATGATTGTTAATCGTAAGGAATGA", "aa": "MYRATKQWFVSIEKILPNILQTLNNDVKSTSFRGIERMHEMIVNRKE", "aa_hexdigest": "376766da85e16d5981cbb307ba0dce18", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5666.559299999999, "isoelectric_point": 9.815134239196777 }, "id": "NKIECMJPJD_770", "locus": "GCA000828735_00757" }, { "type": "cds", "sequence": "contig_1", "start": 451500, "stop": 451769, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATGAGTATGTTAGTGCTAATGACGGAACGGGTTTAGTTCATAATGCGCCTGCTTTTGGTTTAGAAGACTATTATGCATGTAAAAAATATGGTATTGAAACAGTAGTTATGATAGACCAATTTGGTAAATACAATGCATTAGTAAATGATTTAGAATTAGAAAATATGTTTTATGAAGATGCTAATCAAGTTATTTTAAATCGTTTGATGAATGAACATTTATTAATCCATCATGAAGTTATCACTCATAGTGTTGCACATGATTGA", "aa": "MDEYVSANDGTGLVHNAPAFGLEDYYACKKYGIETVVMIDQFGKYNALVNDLELENMFYEDANQVILNRLMNEHLLIHHEVITHSVAHD", "aa_hexdigest": "a8fdf16c57351654cbe27d9395c240fd", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10172.281899999994, "isoelectric_point": 4.609892463684082 }, "id": "NKIECMJPJD_771", "locus": "GCA000828735_00758" }, { "type": "cds", "sequence": "contig_1", "start": 452412, "stop": 452540, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00133.28" ], "nt": "TTGCATGATGGGCCGCCATATGCAAATGGAAATATTCATATTGGTCACGCTTTAAATAAAATACTAAAAGATTTTGTTGTTTCATATCACAATATGAATAATTATTACTCTCCTTATATTCCAGGATGA", "aa": "MHDGPPYANGNIHIGHALNKILKDFVVSYHNMNNYYSPYIPG", "aa_hexdigest": "a46f5e389b03704a67865bef39fd7e6b", "start_type": "TTG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00133.28", "name": "tRNA-synt_1", "length": 42, "aa_cov": 1.0, "hmm_cov": 1.0, "evalue": 1.2116952947081479e-15, "score": 52.69961166381836, "start": 1, "stop": 42 } ], "seq_stats": { "molecular_weight": 4773.323300000002, "isoelectric_point": 6.889363288879395 }, "id": "NKIECMJPJD_772", "locus": "GCA000828735_00759" }, { "type": "cds", "sequence": "contig_1", "start": 452601, "stop": 452696, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGATTATAAAAGCACCCTTAATATGCCTACTACAGGTTTTGAAATGCGTGCTAATTTAAACGTTAAAGAACCAAAAATTCAACAATTTTGA", "aa": "MKDYKSTLNMPTTGFEMRANLNVKEPKIQQF", "aa_hexdigest": "e9edfaab35366b058394bbce546b5a53", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3661.233, "isoelectric_point": 9.398732948303223 }, "id": "NKIECMJPJD_773", "locus": "GCA000828735_00760" }, { "type": "cds", "sequence": "contig_1", "start": 453073, "stop": 453210, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTACACATGAAATTAATACTGAAAAACAAGGACATCAATCTAATAAAAATGTGCGTGAAATTGTAATAGATGCTTCATATAAATCAATATCATCACTAAATTTATCCCTTAAAACATTGATTATTTCAATATGA", "aa": "MATHEINTEKQGHQSNKNVREIVIDASYKSISSLNLSLKTLIISI", "aa_hexdigest": "819b92c20732005434e99cd6e68995ad", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5025.6916999999985, "isoelectric_point": 8.181376457214355 }, "id": "NKIECMJPJD_774", "locus": "GCA000828735_00761" }, { "type": "cds", "sequence": "contig_1", "start": 454020, "stop": 454199, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13276.12" ], "nt": "ATGGATTTAATTGAAAAAATTTGTAAAAGTCATAAGCATTATATTGGTTGTCGTAAAATTCAAAAAATTCTTGAAACACAATATAATGTTAAATTAAATTACAAAACAATTAATTTATATATGAATAAAATGGATTTATGCAAACCTTGTCATCATGAACATCATGAAAAAAAGGAATAA", "aa": "MDLIEKICKSHKHYIGCRKIQKILETQYNVKLNYKTINLYMNKMDLCKPCHHEHHEKKE", "aa_hexdigest": "fcc0b0bc3699ff416b7fd6afc5305fa6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13276.12", "name": "HTH_21", "length": 52, "aa_cov": 0.8813559322033898, "hmm_cov": 1.0, "evalue": 1.068459267303221e-13, "score": 46.85664749145508, "start": 1, "stop": 52 } ], "seq_stats": { "molecular_weight": 7241.514300000001, "isoelectric_point": 9.118102073669434 }, "id": "NKIECMJPJD_775", "locus": "GCA000828735_00762" }, { "type": "cds", "sequence": "contig_1", "start": 454327, "stop": 455454, "strand": "+", "frame": 1, "gene": "dnaJ", "product": "molecular chaperone DnaJ", "db_xrefs": [ "COG:COG0484", "COG:O", "EC:1.8.4.-", "GO:0005515", "GO:0005524", "GO:0005737", "GO:0006260", "GO:0006457", "GO:0008270", "GO:0009408", "GO:0031072", "GO:0042026", "GO:0051082", "GO:0051085", "RefSeq:WP_006689084.1", "SO:0001217", "UniParc:UPI00001295A0", "UniRef:UniRef100_Q9PQ82", "UniRef:UniRef50_Q9PQ82", "UniRef:UniRef90_Q9PQ82" ], "nt": "ATGGCAAAACGTGATTATTATGAAATTTTAGGTGTTAGTAAATCAGCAACTCCTGAAGAGATTAAAGCGGCATTTCGTAAATTAGCAAAAGAACACCATCCTGATCGTAATAAATCTGCTGATGATACTCTTTTTAAAGAAATTAATGAAGCATATGAGGTTTTATCTGATTCTAAAAAACGTGCACAATACGACCAATTTGGCCATGATGGTCCACAAGGTTTTAGCAGTGCTAGTGGGTTTTCTGGGTTTAGTGGAGGATTTGGTGGTGTTGATTTTGATATTAATGACATTTTTGGCAGTTTTTTTAATAATGCTTCTAGTTCACATAATTCATCAAACCAATATGAAACATATGATATTCACTTACGTTTACATTTAGATTTTATGGAAGCTGTTAATGGTATTTCTAAAAATATTAATTATGATCGTAAAATTACGTGTCATAAATGTCAAGGAACAGGTGCAAAAGATCCTAAAGATGTCAAAATTTGCACTAAATGTCACGGTAGAGGAACTAGCATTGAAAATGTTCATTCCTTATTTGGAACAATTCAACAAGAAGTTGAATGTCATGAGTGTGAAGGAACAGGTAAAGTTGCTAGTAGCAAATGTGAACAATGTTATGGTAAAAAAGTTATCAATGAACGTGTTAATTTAACTGTTGAAATCCCAGCAGGTACACGAGAAGGTGAAAAATTATTAGTAAGCAAAAAAGGTAATATTGTCAATAATCAAGAATTTGATCTTTATTTACACATTAGTGTTAAACCATCAAAGTATTTTGCATTAGATGGTTTAGATATTTATTCAGAAACTTACATTGATCCCATCAAAGCAATTGTTGGAGGAATAATTGAAGTAGTAACAATTAATGGTATTAAAACCATTGAAATTCCTTCAAATACTCCTGAAGGTAAAAAATTTAGAATTAGTGGATCAGGAATCGTTAATAAAAAATCAAATATTTTTGGTAAAAAGAATGGAGATTTTTATACTACAATTCGTTATGCAAAGCCAGTTGAATTATCCAAAGATGAAATTGCTTATTTAAAAAATATTTCTACTCGTACAAACCAAAATGTTGAGTATTATAAAAATAAAGTATTAAAGGAGATTAGTAAATAA", "aa": "MAKRDYYEILGVSKSATPEEIKAAFRKLAKEHHPDRNKSADDTLFKEINEAYEVLSDSKKRAQYDQFGHDGPQGFSSASGFSGFSGGFGGVDFDINDIFGSFFNNASSSHNSSNQYETYDIHLRLHLDFMEAVNGISKNINYDRKITCHKCQGTGAKDPKDVKICTKCHGRGTSIENVHSLFGTIQQEVECHECEGTGKVASSKCEQCYGKKVINERVNLTVEIPAGTREGEKLLVSKKGNIVNNQEFDLYLHISVKPSKYFALDGLDIYSETYIDPIKAIVGGIIEVVTINGIKTIEIPSNTPEGKKFRISGSGIVNKKSNIFGKKNGDFYTTIRYAKPVELSKDEIAYLKNISTRTNQNVEYYKNKVLKEISK", "aa_hexdigest": "c17f3981138183607df0f8e99bec84c6", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "ups": { "uniparc_id": "UPI00001295A0", "ncbi_nrp_id": "WP_006689084.1", "uniref100_id": "UniRef100_Q9PQ82", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001295A0", "RefSeq:WP_006689084.1", "UniRef:UniRef100_Q9PQ82" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQ82", "uniref90_id": "UniRef90_Q9PQ82" }, "psc": { "uniref90_id": "UniRef90_Q9PQ82", "gene": "dnaJ", "product": "molecular chaperone DnaJ", "ec_ids": [ "1.8.4.-" ], "uniref50_id": "UniRef50_Q9PQ82", "cog_id": "COG0484", "cog_category": "O", "go_ids": [ "GO:0005515", "GO:0005524", "GO:0005737", "GO:0006260", "GO:0006457", "GO:0008270", "GO:0009408", "GO:0031072", "GO:0042026", "GO:0051082", "GO:0051085" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQ82", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQ82" ], "product": "Chaperone protein DnaJ" }, "genes": [ "dnaJ" ], "id": "NKIECMJPJD_776", "locus": "GCA000828735_00763" }, { "type": "cds", "sequence": "contig_1", "start": 455454, "stop": 456110, "strand": "+", "frame": 3, "gene": "grpE", "product": "Protein GrpE", "db_xrefs": [ "COG:COG0576", "COG:O", "GO:0000774", "GO:0005737", "GO:0006457", "GO:0042803", "GO:0051087", "KEGG:K03687", "RefSeq:WP_006688590.1", "SO:0001217", "UniParc:UPI00000C1C59", "UniRef:UniRef100_Q9PQ83", "UniRef:UniRef50_Q9PQ83", "UniRef:UniRef90_Q9PQ83" ], "nt": "ATGAGCAAAAATAATGAAAACATCAAACACCAAAATAATGACAAAGTTAACAACCAAGTTGATAAAAAAGAAACTAAAAATCATAATAAGCAAGAATTTAAATATAAAGAATTATATGAACATGAATTAAAGAAAAATAAAGAATTACAAAATATCAATACACTTTTAAAAGATAAAAATCAACAGTTAGAAGAGCAAATTAGTCAATTAAATCAAGATTTTATCAAACAATTAGAAACAAAAGCAAAACAAGCACAACAAATTCTAGAACAAAAAGTTAATGAACTAGAAGCACGCCATGAAGCCAAAGTAAATGATGCTGTCTTTAAAATTTTCAAATTTAAAATGGAACCTTTATTAGATGCAATTAATCATTTTACGAAAATTGTAAATCAAAACTATGATGATCCTAAAATCCAAGCTTTCATTGAGGGGTTTAAAATGTTTTCTCAAAACATGATTGATGGTTTAGATAATCTAAAAATTACAAAAATATCTCCACAGGTTAATGATAGTTTAAATGACGAAATAATGGAAGTTTTTGAAGTTGTTGAAAACACAAACAAACCATCAATGCATGTAGTTGAAGTTATTAGTGATGGTTTTAAATATAATGATAAAGTTATTAAATTTGCAGTTGTAAAAGTTGCTAAATAA", "aa": "MSKNNENIKHQNNDKVNNQVDKKETKNHNKQEFKYKELYEHELKKNKELQNINTLLKDKNQQLEEQISQLNQDFIKQLETKAKQAQQILEQKVNELEARHEAKVNDAVFKIFKFKMEPLLDAINHFTKIVNQNYDDPKIQAFIEGFKMFSQNMIDGLDNLKITKISPQVNDSLNDEIMEVFEVVENTNKPSMHVVEVISDGFKYNDKVIKFAVVKVAK", "aa_hexdigest": "1cc3739bdd518632e128a3134d757d1e", "start_type": "ATG", "rbs_motif": "AGGAG/GGAGG", "ups": { "uniparc_id": "UPI00000C1C59", "ncbi_nrp_id": "WP_006688590.1", "uniref100_id": "UniRef100_Q9PQ83", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1C59", "RefSeq:WP_006688590.1", "UniRef:UniRef100_Q9PQ83" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQ83", "uniref90_id": "UniRef90_Q9PQ83" }, "psc": { "uniref90_id": "UniRef90_Q9PQ83", "gene": "grpE", "product": "Protein GrpE", "uniref50_id": "UniRef50_Q9PQ83", "cog_id": "COG0576", "cog_category": "O", "kegg_orthology_id": "K03687", "go_ids": [ "GO:0005737", "GO:0000774", "GO:0042803", "GO:0051087", "GO:0006457" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQ83", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQ83" ], "product": "Protein GrpE" }, "genes": [ "grpE" ], "id": "NKIECMJPJD_777", "locus": "GCA000828735_00764" }, { "type": "cds", "sequence": "contig_1", "start": 456608, "stop": 456721, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTGTATTGGAATTGCATTCTTATATTTTATTAATCCAAAAGCACGTTGTACAAAAAACAATCGTGTTTTATTAATTGTTTTGTCTTACATTTTAATTATTAGTTCAATTTGA", "aa": "MCIGIAFLYFINPKARCTKNNRVLLIVLSYILIISSI", "aa_hexdigest": "a0c5bcfba06033e2e47b15a2eaf4f541", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4215.183799999999, "isoelectric_point": 9.696061134338382 }, "id": "NKIECMJPJD_778", "locus": "GCA000828735_00765" }, { "type": "cds", "sequence": "contig_1", "start": 456995, "stop": 457123, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTGAATTTTTTCTTCTTTGGCTATTTATTAGAATATGTAAAATTAAATAAATTTGAAACATTTAATTTTGCATTAGCGTCAATTTTATTTAATCCTTATGCTTATGTTATTATTGTTAAAAATTGA", "aa": "MVNFFFFGYLLEYVKLNKFETFNFALASILFNPYAYVIIVKN", "aa_hexdigest": "fd80da2b9f13548249a4b0b022ce99e3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5049.918600000002, "isoelectric_point": 8.06333484649658 }, "id": "NKIECMJPJD_779", "locus": "GCA000828735_00766" }, { "type": "cds", "sequence": "contig_1", "start": 457130, "stop": 457399, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTAATAATAAGAAATTTATTAATAAAACATTTGATAATACTAACCAAATACGATTGCAAAATAATTTAAAAACTTATGCTCCTAAATTTTTAAATTTAATTATTATTAATAATATTTTTGTATTAATTATGATTATTAGTTTGTGTATTTTCTTTTCTGATCCACATTTTTTATATCGAAACGATGTCATTTCGAACCAATATCGCCAATTTATATTTACATATCAGCAAACAGCAATCGCAATTGTTTTATATGTTTTAGCATGA", "aa": "MINNKKFINKTFDNTNQIRLQNNLKTYAPKFLNLIIINNIFVLIMIISLCIFFSDPHFLYRNDVISNQYRQFIFTYQQTAIAIVLYVLA", "aa_hexdigest": "e30e35134d54b9eaa06e30c8a28485a3", "start_type": "TTG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10611.406699999985, "isoelectric_point": 9.66582546234131 }, "id": "NKIECMJPJD_780", "locus": "GCA000828735_00767" }, { "type": "cds", "sequence": "contig_1", "start": 457614, "stop": 458240, "strand": "+", "frame": 3, "gene": "plsY", "product": "glycerol-3-phosphate 1-O-acyltransferase PlsY", "db_xrefs": [ "COG:COG0344", "COG:I", "EC:2.3.1.15", "GO:0005886", "GO:0006654", "GO:0008374", "GO:0008654", "GO:0043772", "SO:0001217", "UniRef:UniRef50_Q9PQ85", "UniRef:UniRef90_Q9PQ85" ], "nt": "ATGGATCAAGTATATAACATTGTCATGGCTTATATTCTTACATTAATCATATCTCCTTTATATTCGTATTTAATTGGTAGTTTAAATGCAAGCATCATTTTATCCTTAGTTTTTAAAAAACAAGATGTTCGTCTTTTTGCTTCTAAAAATGCAGGCATGACTAATATGATACGTGTTCACGGAAAAAAATTAGGTATATTAACGCTTTTTTTAGATATTATTAAACCAATCACCACAGTTAGTTTAACTTACATTATTTACAAATATGCTTTAGACGCTCCATTTGATTTAAGTAATGGTTTTAATCAAGCCATTTTAGTTTATTTTGGTGGTATTTTCACCATTATTGGTCATTGTTACCCAATCTTCTTTAAATTTCAAGGCGGGAAGGGAGTTGCATGTTATGGCGGTTTTTTAATTACAGTTGATCCAATAGTAGCGCTAATTGGAATCATTACACTATTGGTCATTTTACTAATTACTAAATATATGTCTTTATCTGCTATGATTACTGCAACTTTCACTTGTTTTTTAGTATTAGTTCCAGGAATTAATTACATACCATACTATAATCAAAATTTCACTACGTATTTTTTTGATTTAAATTATGTTATAAAAGGAATCTGA", "aa": "MDQVYNIVMAYILTLIISPLYSYLIGSLNASIILSLVFKKQDVRLFASKNAGMTNMIRVHGKKLGILTLFLDIIKPITTVSLTYIIYKYALDAPFDLSNGFNQAILVYFGGIFTIIGHCYPIFFKFQGGKGVACYGGFLITVDPIVALIGIITLLVILLITKYMSLSAMITATFTCFLVLVPGINYIPYYNQNFTTYFFDLNYVIKGI", "aa_hexdigest": "7be9138216a9cf234d3a167fec29b3f1", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_Q9PQ85", "query_cov": 1.0, "subject_cov": 0.8093385214007782, "identity": 1.0, "score": 393.0, "evalue": 2.94e-137, "valid": true, "gene": "plsY", "product": "glycerol-3-phosphate 1-O-acyltransferase PlsY", "ec_ids": [ "2.3.1.15" ], "uniref50_id": "UniRef50_Q9PQ85", "cog_id": "COG0344", "cog_category": "I", "go_ids": [ "GO:0005886", "GO:0006654", "GO:0008374", "GO:0008654", "GO:0043772" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQ85", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQ85" ], "product": "Glycerol-3-phosphate acyltransferase" }, "genes": [ "plsY" ], "id": "NKIECMJPJD_781", "locus": "GCA000828735_00768" }, { "type": "cds", "sequence": "contig_1", "start": 458448, "stop": 458996, "strand": "+", "frame": 3, "gene": "iS285", "product": "Mutator family transposase", "db_xrefs": [ "COG:COG3328", "COG:X", "SO:0001217", "UniRef:UniRef50_Q9PQ86", "UniRef:UniRef90_Q9PQ86" ], "nt": "ATGGACAAAAAGAATATTTTTACAATTCTAGAGAAGTTAGTTAAAAAATACAAGTATTTGCACAAACCAATTCTAGATGACTATATTAGTACTTTTACAAGAACCTTTTTAGAATATATATTGACAATCGAAATGAAATATCATTTAAATTATGAACGTTTTGAACGTTATTTAAGTAGTAATTCTAAAAACTATCGTAATGGTTTTTCTAAAAAACAACTGTATATGAACGATTTAAAAGTTTCAATTAATATTCCACGTGATCGTAATGGAGCTTTTGAATCAAAAATTATTTCAAAATACCAAGTTGATATTTCACATTTTGAATATCGCTTATTATCGTTGGGAATCAATAATTTACAATTAAATGAATATGAAAAAATATTAGAAGATATTTATAATATTTATAATGACAAAATGGTTGATAAACTATGTAATGAAATTGCTTTAAAAATTTTTGATTATTTAGAGAATAAAAAACAAGAACTAAATAATATAGATGTTGATCAAATTTTTATCGATGGTTTTAATTTATCATCAAATAAATGA", "aa": "MDKKNIFTILEKLVKKYKYLHKPILDDYISTFTRTFLEYILTIEMKYHLNYERFERYLSSNSKNYRNGFSKKQLYMNDLKVSINIPRDRNGAFESKIISKYQVDISHFEYRLLSLGINNLQLNEYEKILEDIYNIYNDKMVDKLCNEIALKIFDYLENKKQELNNIDVDQIFIDGFNLSSNK", "aa_hexdigest": "ad152dbc0db9884a63a0f6e41332e6d5", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "genes": [ "iS285" ], "truncated": "3-prime", "pseudogene": { "start": 458448, "stop": 459254, "observations": { "mutation": [ 458994, 459051, 459078, 459081 ], "stop": [ 458994, 459051, 459078, 459081 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQ86", "query_cov": 1.0, "subject_cov": 0.6791044776119403, "identity": 1.0, "score": 361.0, "evalue": 4.65e-125, "gene_start": 1, "gene_end": 182, "reference_start": 1, "reference_end": 182, "reference_sequence": "MDKKNIFTILEKLVKKYKYLHKPILDDYISTFTRTFLEYILTIEMKYHLNYERFERYLSSNSKNYRNGFSKKQLYMNDLKVSINIPRDRNGAFESKIISKYQVDISHFEYRLLSLGINNLQLNEYEKILEDIYNIYNDKMVDKLCNEIALKIFDYLENKKQELNNIDVDQIFIDGFNLSSNKWLTIVITKDLEKRLTILGFWVINNLEDKWWNDIIIKISKYHPQVINYKVIFKDNDELLISLKNHTSNNFERARFVFINKSILNQKN" }, "paralog": false, "identity": 0.9813432835820896, "score": 519.0, "evalue": 1.18e-189, "description": "Internal stop codon at 458994, 459051, 459078, 459081. Nonsense mutation around 458994, 459051, 459078, 459081.", "psc": { "uniref90_id": "UniRef90_Q9PQ86", "gene": "IS285", "product": "Mutator family transposase", "uniref50_id": "UniRef50_Q9PQ86", "cog_id": "COG3328", "cog_category": "X", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQ86", "UniRef:UniRef50_Q9PQ86", "COG:COG3328", "COG:X" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQ86", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQ86" ], "product": "Mutator family transposase" } }, "id": "NKIECMJPJD_782", "locus": "GCA000828735_00769" }, { "type": "cds", "sequence": "contig_1", "start": 459483, "stop": 459668, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTTATTCTCAAGACAATCTTAGCAAGGGTCCACAAGTTTATGCTAGTTCAAAAATATATGACAAATCAAAAAATGAACGTAAAGAATCATATGTTTCTGGAGACAACGTCTATAATTCATCACGTTTTGAATTTGGTGGAGATTTAAACGCTGCTATTTATAGTGTCTATGGTAAAAAATAA", "aa": "MFYSQDNLSKGPQVYASSKIYDKSKNERKESYVSGDNVYNSSRFEFGGDLNAAIYSVYGKK", "aa_hexdigest": "95d0bbfbfecc6f33c7230434f1d155b7", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6932.518400000004, "isoelectric_point": 8.891302299499515 }, "id": "NKIECMJPJD_783", "locus": "GCA000828735_00770" }, { "type": "cds", "sequence": "contig_1", "start": 459858, "stop": 460379, "strand": "-", "frame": 1, "gene": null, "product": "Lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T243", "UniRef:UniRef90_A0AAC9T243" ], "nt": "ATGAAAAAAGCAATTTTGATTTCCATTTCTTCATTAATTGGGATTAGTGCGATTGTCGCAACTAGTACTTTTTTGTCATTATCAGCCAAAACAAATATTTATGCAAAATTAATAAAATCAAGCGATCTTAATCAAAGTTTAAATTACACTAATTTATCAGATGCTAATATTGGAATTAAAGAAATGCTTTTAGGTACTAATAAAATTAATAATGGAAATTATGTTTTATATATTGGAACACAATCAAACCTTGATAATTTAAATTTTGTTTATGATAATCAAACTAACCATATTAATTCGATTAGTGATTTAAAATATAATGATAATTTGAAGTTTAATGGATCATTAGCAAAATCAATTAATAATGTCAAAAATTATGCAGATAAAGGTGTTTATGAGCATGTCCCAGAATTCTATAGTTTTATTGATTTAATAGATACTAATGTTTTTAAACAAAAACAAGATTATGAAAATTTAATTAAACAAAATAAATCAAGCACTATTAAAGAAGATCAAAATTGA", "aa": "MKKAILISISSLIGISAIVATSTFLSLSAKTNIYAKLIKSSDLNQSLNYTNLSDANIGIKEMLLGTNKINNGNYVLYIGTQSNLDNLNFVYDNQTNHINSISDLKYNDNLKFNGSLAKSINNVKNYADKGVYEHVPEFYSFIDLIDTNVFKQKQDYENLIKQNKSSTIKEDQN", "aa_hexdigest": "4f2860afda37cc0003b20b66e974f848", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 459555, "stop": 460379, "observations": { "mutation": [ 459860 ], "stop": [ 459860 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAC9T243", "query_cov": 1.0, "subject_cov": 0.5805369127516778, "identity": 0.9940000000000001, "score": 318.0, "evalue": 1.43e-107, "gene_start": 1, "gene_end": 173, "reference_start": 1, "reference_end": 173, "reference_sequence": "MKKAILISISSLIGISAIVATSTFLSLSAKTNIYAKLIKSSDLNQSLNYTNLSDANIGIKEMLLGTNKINNGNYVLYIGTQSNLDNLNFVYDNQTNHINSISDLKYNDNLKFNGSLAKSINNVKNYADKGVYEHVPEFYSFIDLMDTNVFKQKQDYENLIKQNKSSTIKEDQNWANNAPTDYSFDVNKKYKDKSGREVYFRNDTQAVKFREILNFLKKYLSKEKLIELSTSQTPGIVLFYSQDNLSKGPQVYASSKIYDKSKNERKESYVSGDNVYNSSRFEFGGDLNAAIYSVYGKK" }, "paralog": false, "identity": 0.9927007299270073, "score": 535.0, "evalue": 1.44e-195, "description": "Internal stop codon at 459860. Nonsense mutation around 459860.", "psc": { "uniref90_id": "UniRef90_A0AAC9T243", "product": "Lipoprotein", "uniref50_id": "UniRef50_A0AAC9T243", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAC9T243", "UniRef:UniRef50_A0AAC9T243" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAC9T243", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T243" ], "product": "Lipoprotein" } }, "id": "NKIECMJPJD_784", "locus": "GCA000828735_00771" }, { "type": "cds", "sequence": "contig_1", "start": 460488, "stop": 460757, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02540.23", "PFAM:PF03054.22" ], "nt": "ATGGAAGTTAAAGTAAAAAAACGTGTCGTTGTTGGCTTGTCTGGTGGCGTAGATTCTTCTGTTAGTGCTTTATTATTAAAACAAGAAGGCTATGAAGTGATTGGTTTATTTATGAGTAATTGGGATACAATTGCAAATTTTGAAAATAACCATGAATTTAATAAAACGCATCAGGGTTGTGAGAGTGAATTAGATTACCAAGATGCTCAGGCTGTTGCTCAAAAGATCGGTATTCCATTATATCGTGTTGAATTTATTAAAGAATATTGA", "aa": "MEVKVKKRVVVGLSGGVDSSVSALLLKQEGYEVIGLFMSNWDTIANFENNHEFNKTHQGCESELDYQDAQAVAQKIGIPLYRVEFIKEY", "aa_hexdigest": "360c470393423fc673e3bf97fd191c01", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A4R2K1R8", "query_cov": 0.9325842696629213, "subject_cov": 0.9120879120879121, "identity": 0.506, "score": 70.5, "evalue": 4.21e-14, "valid": false, "product": "tRNA methyltransferase", "uniref50_id": "UniRef50_J9FDV7" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02540.23", "name": "NAD_synthase", "length": 79, "aa_cov": 0.8876404494382022, "hmm_cov": 0.7974683544303798, "evalue": 4.470670479935845e-09, "score": 31.331361770629883, "start": 6, "stop": 84 }, { "id": "PF03054.22", "name": "tRNA_Me_trans", "length": 83, "aa_cov": 0.9325842696629213, "hmm_cov": 0.9156626506024096, "evalue": 2.3396170533019327e-23, "score": 78.44087219238281, "start": 7, "stop": 89 } ], "seq_stats": { "molecular_weight": 10054.275399999993, "isoelectric_point": 5.045563316345214 }, "id": "NKIECMJPJD_785", "locus": "GCA000828735_00772" }, { "type": "cds", "sequence": "contig_1", "start": 460896, "stop": 461645, "strand": "+", "frame": 3, "gene": "mnmA", "product": "tRNA 2-thiouridine(34) synthase MnmA", "db_xrefs": [ "COG:COG0482", "COG:J", "EC:2.8.1.13", "GO:0000049", "GO:0002143", "GO:0005524", "GO:0005737", "GO:0008033", "GO:0016740", "GO:0016783", "GO:0103016", "KEGG:K00566", "SO:0001217", "UniRef:UniRef50_Q9PQ88", "UniRef:UniRef90_Q9PQ88" ], "nt": "ATGGGTCATTATGCAAGAGTTAAGCATGATAGGAATTCATCTTTTTTACTAAAAGCTATTGATACAAATAAGGATCAAACTTATTTTTTGTGTAATTTAAACCAAAATCAACTACAGAATGTGCTATTTCCTATTGGGCATTTAACTAAACTACAGGTGCGCGCTATTGCTAAAAAACACGGTTTGATTACAGCTAATAAGAAAGATTCAACAGGGATTTGTTTTATTGGTGAACGTAATTTTAAAACTTTTTTACAAAATTATATTCCTAACCAACCAGGGCAAATTATAAATATTGTCAATAATCAAATTATTGGTCATCACATAGGAACAATGTATTATACAATTGGACAACGAAAAGGATTGAATCTAGGTGGCATGAATGAGAGAATGTTTGTTTGTGATAAAGATATCGATAAAAAAATAATCTACGTTGCCCCTTCAAGTTTTGAAAAACAATATTTAATTTCAACTCAAGCATTAATTGAAAATATAAATTTTATTGAACCATATAATCCTCAAATTCCAATTATGGTACGCTTTCGTCACCGACAAGATTTAATAATAGTAAACGATTTTTTACCTATTAAAAATACCAAAAATGTACTAATAAATTATGAGTCAGCTAGAGCTATTACTCCTGGGCAATATGCTGTCTTTTACCAAAATGATCATTGCATTGGCGGTGGAATTGTTTCTAAAACTAATATTGGACATCAAAAAGTGGATTTTTTGGTATATAAATCATAG", "aa": "MGHYARVKHDRNSSFLLKAIDTNKDQTYFLCNLNQNQLQNVLFPIGHLTKLQVRAIAKKHGLITANKKDSTGICFIGERNFKTFLQNYIPNQPGQIINIVNNQIIGHHIGTMYYTIGQRKGLNLGGMNERMFVCDKDIDKKIIYVAPSSFEKQYLISTQALIENINFIEPYNPQIPIMVRFRHRQDLIIVNDFLPIKNTKNVLINYESARAITPGQYAVFYQNDHCIGGGIVSKTNIGHQKVDFLVYKS", "aa_hexdigest": "4a2d77648f55814326e30c0f3af420ae", "start_type": "ATG", "rbs_motif": null, "genes": [ "mnmA" ], "truncated": "5-prime", "pseudogene": { "start": 460596, "stop": 461645, "observations": { "mutation": [ 460755 ], "stop": [ 460755 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQ88", "query_cov": 1.0, "subject_cov": 0.6467532467532467, "identity": 1.0, "score": 509.0, "evalue": 1.06e-180, "gene_start": 1, "gene_end": 249, "reference_start": 137, "reference_end": 385, "reference_sequence": "MEVKVKKRVVVGLSGGVDSSVSALLLKQEGYEVIGLFMSNWDTIANFENNHEFNKTHQGCESELDYQDAQAVAQKIGIPLYRVEFIKEYWDNVFEYFLSEYQKNRTPNPDILCNQFIKFDSFLNYAKNELQADYIAMGHYARVKHDRNSSFLLKAIDTNKDQTYFLCNLNQNQLQNVLFPIGHLTKLQVRAIAKKHGLITANKKDSTGICFIGERNFKTFLQNYIPNQPGQIINIVNNQIIGHHIGTMYYTIGQRKGLNLGGMNERMFVCDKDIDKKIIYVAPSSFEKQYLISTQALIENINFIEPYNPQIPIMVRFRHRQDLIIVNDFLPIKNTKNVLINYESARAITPGQYAVFYQNDHCIGGGIVSKTNIGHQKVDFLVYKS" }, "paralog": false, "identity": 0.997134670487106, "score": 716.0, "evalue": 1.89e-264, "description": "Internal stop codon at 460755. Nonsense mutation around 460755.", "psc": { "uniref90_id": "UniRef90_Q9PQ88", "gene": "mnmA", "product": "tRNA 2-thiouridine(34) synthase MnmA", "ec_ids": [ "2.8.1.13" ], "uniref50_id": "UniRef50_Q9PQ88", "cog_id": "COG0482", "cog_category": "J", "kegg_orthology_id": "K00566", "go_ids": [ "GO:0000049", "GO:0002143", "GO:0005524", "GO:0005737", "GO:0008033", "GO:0016740", "GO:0016783", "GO:0103016" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQ88", "EC:2.8.1.13", "UniRef:UniRef50_Q9PQ88", "COG:COG0482", "COG:J", "KEGG:K00566", "GO:0000049", "GO:0002143", "GO:0005524", "GO:0005737", "GO:0008033", "GO:0016740", "GO:0016783", "GO:0103016" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQ88", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQ88" ], "product": "tRNA-specific 2-thiouridylase MnmA" } }, "id": "NKIECMJPJD_786", "locus": "GCA000828735_00773" }, { "type": "cds", "sequence": "contig_1", "start": 461669, "stop": 462181, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATTGTTTGTCAAAACAAAACAATATTATTTTAAAAATGAGGATAATAAAAATATGTTTAAGAAAAATAAAAAATCTTTAGCAGTATTGCTAGTAGGTTTTTCAACTGTTTTAATTTCAAGTATTGCGGTTGCTGCTACAGCATGTGCAAGAAAAGACAATAACGTTAAAGAGGAAGGAAAAACAACACTTAATAAGTTAATTTCAAAAAAAGCTCCAAAACAAAATGCAACTAAATCTAGTGAAAAATATATCTTAAGTGATGTTAATAATAGTAGTACTTTTTTTAATTCACTAACAGACCCTTATAAATGTTTTGAAATGTTTGCAAAATCATTGGATGATGCAGGTTATGAAATTACATCACATGAACCTTTGAAAAATAGCGGTAAAACAAGTTTTTTAGAGTATGCTAAAGAACTTAATAATAAGATTCGTGAATATGTTAGAAAAGTTGCTAATTTACCAACTATTAAAAAATTAAGAGATAATAATACAACTGTTTTATGA", "aa": "MKLFVKTKQYYFKNEDNKNMFKKNKKSLAVLLVGFSTVLISSIAVAATACARKDNNVKEEGKTTLNKLISKKAPKQNATKSSEKYILSDVNNSSTFFNSLTDPYKCFEMFAKSLDDAGYEITSHEPLKNSGKTSFLEYAKELNNKIREYVRKVANLPTIKKLRDNNTTVL", "aa_hexdigest": "631ad42b67df289ed960695f42fd8e63", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19284.000100000012, "isoelectric_point": 9.69799518585205 }, "id": "NKIECMJPJD_787", "locus": "GCA000828735_00774" }, { "type": "cds", "sequence": "contig_1", "start": 462413, "stop": 462601, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTATCAATGCAAGAAGCTTTTTATGGAACTGCGGATTATGTTTTCTTTAATTATGACTCAAACAATCTTGAAGGCAATGAAGATGGAGAGATTTTAAAATCAAATTTTGAAAAATTCTTTAAGAAAACTGTTAATAATAACAAAATTATTCCAGTTGATTTTCAATTTGAATATTCTTCAATTTGA", "aa": "MLSMQEAFYGTADYVFFNYDSNNLEGNEDGEILKSNFEKFFKKTVNNNKIIPVDFQFEYSSI", "aa_hexdigest": "b257a0a80c8681b5415a649de8f46730", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7292.980200000001, "isoelectric_point": 4.31762638092041 }, "id": "NKIECMJPJD_788", "locus": "GCA000828735_00775" }, { "type": "cds", "sequence": "contig_1", "start": 462794, "stop": 463141, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGCATTGGGAATTCAACCAGACTATGCTTCATTCGTTAAAGAAAAAGGAAAAACATATACTAAACTACCAGGATTTTTAGCTGATTTAGTAGATGAAACAAAAACCGAATTTAAAGATTTCACTGGTGATGCTCAAAAAGTTGCTGGTATGAATTTATATGCTCTAGGTGGTAATAATTCTCATATTAACCGTTCTTTTAAAACTCTTATTGGCAATGCAATACCAAAAGCTGTTCCTACTGAACGTGGCGATTCAAATAATTCTCGTTCACCAATTAATTTTGAATTCTATGATCGTACAACAAACACATTACAATCTGTTGAAGGAACAAAAGTTGTAAGTTGA", "aa": "MALGIQPDYASFVKEKGKTYTKLPGFLADLVDETKTEFKDFTGDAQKVAGMNLYALGGNNSHINRSFKTLIGNAIPKAVPTERGDSNNSRSPINFEFYDRTTNTLQSVEGTKVVS", "aa_hexdigest": "bf6f46aba7dd3528870c302ea6b1cb1d", "start_type": "GTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12596.967899999985, "isoelectric_point": 7.931303596496583 }, "id": "NKIECMJPJD_789", "locus": "GCA000828735_00776" }, { "type": "cds", "sequence": "contig_1", "start": 463284, "stop": 463775, "strand": "+", "frame": 3, "gene": "fepD", "product": "Ferrichrome ABC transporter (Permease)", "db_xrefs": [ "COG:COG0609", "COG:P", "SO:0001217", "UniRef:UniRef50_Q9PQ90", "UniRef:UniRef90_Q9PQ90" ], "nt": "GTGCTAATTTGTTATTGTCTTTTTGATGGTAAAGAGTTAATGAATTTTAAAAATGTAGGTGAAGAAATAAATGAGGGAAGTGCCCCTTATATTCGTAAATTTATTGCTACTCCAATTGCAATCTTTTTATCTGCATTAACATTATCATTTAGTGGGTACAGTATGCAAGTAGTTTCACGAAACCCACTAGCTTCACCAACTACATTAGGATATTTACCTGCTGCAATTTTAGGTTTAGCAATTAGTAAACTTGCTATTAATCAAATTTTATATCTTCCTTTCATTATTGGTATTGTTTTTGCTTCATGTCTAATTGTAATTAATTTTTTCTTGGTAAAAGGAAATGCTTTAGAAGCAAGTTTTAAACCAATTTTAGTAGGATTTGCAATTGGAGGTATCATTACTGGTATTAATGTATTATTAGAAGACCTTGTAAAAGATATAAACATTAAAATTACAGGTTTCGTTGAACCTCCAATTAATTTTCAATGA", "aa": "MLICYCLFDGKELMNFKNVGEEINEGSAPYIRKFIATPIAIFLSALTLSFSGYSMQVVSRNPLASPTTLGYLPAAILGLAISKLAINQILYLPFIIGIVFASCLIVINFFLVKGNALEASFKPILVGFAIGGIITGINVLLEDLVKDINIKITGFVEPPINFQ", "aa_hexdigest": "46a47780a0bf48e601e74b2bc7b1be1e", "start_type": "GTG", "rbs_motif": null, "genes": [ "fepD" ], "truncated": "both", "pseudogene": { "start": 463185, "stop": 464078, "observations": { "mutation": [ 463227, 463773 ], "stop": [ 463227, 463773 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQ90", "query_cov": 1.0, "subject_cov": 0.4808259587020649, "identity": 0.9940000000000001, "score": 310.0, "evalue": 5.74e-104, "gene_start": 1, "gene_end": 163, "reference_start": 34, "reference_end": 196, "reference_sequence": "MFKNKSLKFKNKLDWKTHFRRLITSLLIILSICVLICYCLFDGKELMNFKNVGEEINEGSAPYIRKFIATPIAIFLSALTLSFSGYSMQVVSRNPLASPTTLGYLPAAILGLAISKLAINQILYLPFIIGIVFASCLIVINFFLVKGNALEASFKPILVGFAIGGIITGINVLLEDLVKDINIKITGFVEPPINFQWQQLCIGGPLIIISSLANLFMAPYYTIIAKDYLLAKSLGIKVNLIFWLTAFFAVVATVSSIILIGVLTLLGMIAPHVARILNPKGNSFKQLLLSFVISLLLLTSSRWLINVYNQFDINLFSAIAALPVFAYIFVSKQYRRNVE" }, "paralog": false, "identity": 0.9932659932659933, "score": 564.0, "evalue": 1.18e-206, "description": "Internal stop codon at 463227, 463773. Nonsense mutation around 463227, 463773.", "psc": { "uniref90_id": "UniRef90_Q9PQ90", "gene": "FepD", "product": "Ferrichrome ABC transporter (Permease)", "uniref50_id": "UniRef50_Q9PQ90", "cog_id": "COG0609", "cog_category": "P", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQ90", "UniRef:UniRef50_Q9PQ90", "COG:COG0609", "COG:P" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQ90", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQ90" ], "product": "Ferrichrome ABC transporter (Permease)" } }, "id": "NKIECMJPJD_790", "locus": "GCA000828735_00777" }, { "type": "cds", "sequence": "contig_1", "start": 463833, "stop": 464093, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCGCCATATTATACAATCATTGCTAAAGATTATTTATTAGCAAAATCTTTAGGAATTAAAGTTAATTTGATTTTTTGGCTAACAGCTTTTTTTGCAGTTGTCGCAACTGTTTCAAGCATCATTTTAATTGGTGTTTTAACACTATTAGGAATGATAGCGCCTCATGTGGCACGAATATTAAATCCAAAAGGCAATAGTTTTAAACAATTATTATTATCATTTGTAATTTCATTATTACTATTAACATCTTCACGTTGA", "aa": "MAPYYTIIAKDYLLAKSLGIKVNLIFWLTAFFAVVATVSSIILIGVLTLLGMIAPHVARILNPKGNSFKQLLLSFVISLLLLTSSR", "aa_hexdigest": "23429a59a8a72dbded9f730da4c9bab2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9407.386499999993, "isoelectric_point": 10.214580345153806 }, "id": "NKIECMJPJD_791", "locus": "GCA000828735_00778" }, { "type": "cds", "sequence": "contig_1", "start": 464207, "stop": 464422, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAATGAAATTTAATTTTTTTAAAGATAAACAAAATATATTAGGACAAAATCACATCTTAGAATTAAATAAAAAAAGTTTTATTCCATATCATTTTAAACCTTTGTTAATAACAATAACTTTATTGATTGCTTTATTAGTTGTCATTATTTTAATTTTTTCTGTTACCTTATCTAATAAGTTAGGCTTTAGTGAATTAGCTAAAGTAATATGA", "aa": "MKMKFNFFKDKQNILGQNHILELNKKSFIPYHFKPLLITITLLIALLVVIILIFSVTLSNKLGFSELAKVI", "aa_hexdigest": "19a54b5e00e7a8a90e63c90bb80eaa98", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8202.971699999993, "isoelectric_point": 10.000222969055176 }, "id": "NKIECMJPJD_792", "locus": "GCA000828735_00779" }, { "type": "cds", "sequence": "contig_1", "start": 464654, "stop": 464773, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTTTATATTTCTTTGTTTCACGTCAAAAAGAAAACTTTAATTACAAAAAACTAATTTTAATAGGAGTTATTTTAACATTCTTTTTAATTGCAATTGCACAAAGTATTCGTGGTTGA", "aa": "MVLYFFVSRQKENFNYKKLILIGVILTFFLIAIAQSIRG", "aa_hexdigest": "731c41a2692460990a13f3257a234202", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4595.5361, "isoelectric_point": 10.165906715393067 }, "id": "NKIECMJPJD_793", "locus": "GCA000828735_00780" }, { "type": "cds", "sequence": "contig_1", "start": 464794, "stop": 464916, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTAGTTTGTATGAGTTAAATAATAATCATAATAAGCCTAAAATAACAAAGCTTGCTGCAATAATTAATGAATTTAATGGTGCTTTTTGTACTGACCCTACAATATGGCCAACAACATAA", "aa": "MFSLYELNNNHNKPKITKLAAIINEFNGAFCTDPTIWPTT", "aa_hexdigest": "8f8d397a2d52e06897065f2980760b22", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4569.176500000001, "isoelectric_point": 6.510758781433105 }, "id": "NKIECMJPJD_794", "locus": "GCA000828735_00781" }, { "type": "cds", "sequence": "contig_1", "start": 465002, "stop": 465154, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTAGGTGCAAGTTATTCAATATCAGGCGATTTTGTTTATGTTGGTCTATTAGCGGGTAATGCAGCAATGCGTCGTCGTAATAATAGTTTCACATACGGAATATTAAATTCAGGGATATATGGAATGTTGTCAACATTAATTACTTATTGA", "aa": "MVGASYSISGDFVYVGLLAGNAAMRRRNNSFTYGILNSGIYGMLSTLITY", "aa_hexdigest": "a4b25d1b663b30269ebb40567f688b7a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5387.129399999998, "isoelectric_point": 9.39608974456787 }, "id": "NKIECMJPJD_795", "locus": "GCA000828735_00782" }, { "type": "cds", "sequence": "contig_1", "start": 465346, "stop": 465951, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_006688702.1", "SO:0001217", "UniParc:UPI00000C1C53", "UniRef:UniRef100_A0AAC9X6Y3", "UniRef:UniRef50_A0AAC9X6Y3", "UniRef:UniRef90_A0AAC9X6Y3" ], "nt": "ATGAAATTTAAGAAGTTTGATAGTAAAAAATCGATTTTACGTTTTTTACAAGTTGATGAAACAAAACGTGAAATTAATTTGTCTTTTAATGACACAACTGATTTTATTTTTTTTCTAAAAGAAATTGCTGAAGATCCTAATATTGCTAAAAAGAATGCTAAAAAATTAACTAGCTTATACATTGAAATTAGTAATAAACTGGGTAATGATGACAACACTGATTTAAGCGTTTATGAAACAAAATGTAATAATTTAATTGAAGCCTGTGTTATTGATGAAGCAATCTATTTAGTTAATATGGCAAAGAACATAGTCATTTTCTTAGCTCATAACAAACTTGATCTTTTACAAAAAGATTTTTTTGATAAATTATTATTAATAGAATCGCATTTTAATTTTGATCAAAATCTTACTTATTCATATATTAAAAACTCATCAATTGCGTTATTGATATTTCATTTAAAGCAATTTATTAACATTTATGATGAATTTTTAAACAATTTTGAAAATGTTATTAATCATAGTTTAGAAAATTCAATTTCAACTAAAAATAAATTAACCCAACAAATCAATCATTTTAATACTATAAAACGTCGTCCTTCATAA", "aa": "MKFKKFDSKKSILRFLQVDETKREINLSFNDTTDFIFFLKEIAEDPNIAKKNAKKLTSLYIEISNKLGNDDNTDLSVYETKCNNLIEACVIDEAIYLVNMAKNIVIFLAHNKLDLLQKDFFDKLLLIESHFNFDQNLTYSYIKNSSIALLIFHLKQFINIYDEFLNNFENVINHSLENSISTKNKLTQQINHFNTIKRRPS", "aa_hexdigest": "2757d3ca09721a74467c4db4b5d42efa", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI00000C1C53", "ncbi_nrp_id": "WP_006688702.1", "uniref100_id": "UniRef100_A0AAC9X6Y3", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1C53", "RefSeq:WP_006688702.1", "UniRef:UniRef100_A0AAC9X6Y3" ] }, "ips": { "uniref100_id": "UniRef100_A0AAC9X6Y3", "uniref90_id": "UniRef90_A0AAC9X6Y3" }, "psc": { "uniref90_id": "UniRef90_A0AAC9X6Y3", "uniref50_id": "UniRef50_A0AAC9X6Y3" }, "pscc": { "uniref50_id": "UniRef50_A0AAC9X6Y3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9X6Y3" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 23615.80770000003, "isoelectric_point": 6.964447593688964 }, "id": "NKIECMJPJD_796", "locus": "GCA000828735_00783" }, { "type": "cds", "sequence": "contig_1", "start": 466321, "stop": 466707, "strand": "+", "frame": 1, "gene": "rnhC", "product": "Ribonuclease HIII", "db_xrefs": [ "COG:COG1039", "COG:L", "EC:3.1.26.4", "GO:0000287", "GO:0003723", "GO:0004523", "GO:0005737", "GO:0006298", "GO:0032299", "GO:0043137", "SO:0001217", "UniRef:UniRef50_Q9PQ93", "UniRef:UniRef90_Q9PQ93" ], "nt": "ATGAGCCAATCAGAAATTTATGAAAGTGCCCAAGTAATTATTGGGTCTGATGAGGTTGGAGTTGGCGATTTATTTGGAGGTATAGTTGTTTGCGCAGTGAGTTTAAAAAAAAGTGATTTTAAAAAAATAAATCATTTAAAAATCATAGATTCTAAAAAACTCAATGATCAGCAAATGCAAGAAATATATCAACAAATTAAAAATCAGATTTCATATACAATTGTTTCTTATAATCCTAAAGAATACAATGAATTAATTAAAGAATACAATAATGCACATATTCTTAAAACTATTTTACATTATAAAGCATTACAAAGTGAAATTCACAAACACGCTAAATATTCAATTTTTAGTGTTGTTGATGCTTTTAGTTCATTAAAAAACTGA", "aa": "MSQSEIYESAQVIIGSDEVGVGDLFGGIVVCAVSLKKSDFKKINHLKIIDSKKLNDQQMQEIYQQIKNQISYTIVSYNPKEYNELIKEYNNAHILKTILHYKALQSEIHKHAKYSIFSVVDAFSSLKN", "aa_hexdigest": "a0ca1849fa8ae7a5c8643cd670a0477f", "start_type": "ATG", "rbs_motif": null, "genes": [ "rnhC" ], "truncated": "both", "pseudogene": { "start": 466051, "stop": 467001, "observations": { "mutation": [ 466108, 466705 ], "stop": [ 466108, 466705 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQ93", "query_cov": 1.0, "subject_cov": 0.4050632911392405, "identity": 1.0, "score": 246.0, "evalue": 1.02e-79, "gene_start": 1, "gene_end": 128, "reference_start": 91, "reference_end": 218, "reference_sequence": "MAIVSVKLTSNQVDKFLILWRPYLVHNNHNYAKYTFKIDGTLIIIYNTNTCTINTHDINSFLNTFLKREDLTLFNVIKQQVNTKKNNQNVMSQSEIYESAQVIIGSDEVGVGDLFGGIVVCAVSLKKSDFKKINHLKIIDSKKLNDQQMQEIYQQIKNQISYTIVSYNPKEYNELIKEYNNAHILKTILHYKALQSEIHKHAKYSIFSVVDAFSSLKNWNQYLQKVNFQPYEPNLLIPKAESIYTSVALASIIARVMFLKMIAIIEEQFNVKIPLGSSNPLVNDVASNIFQKFGLKILKQVAKEHFSNFQQIIKNK" }, "paralog": false, "identity": 0.9936708860759493, "score": 613.0, "evalue": 3.95e-227, "description": "Internal stop codon at 466108, 466705. Nonsense mutation around 466108, 466705.", "psc": { "uniref90_id": "UniRef90_Q9PQ93", "gene": "rnhC", "product": "Ribonuclease HIII", "ec_ids": [ "3.1.26.4" ], "uniref50_id": "UniRef50_Q9PQ93", "cog_id": "COG1039", "cog_category": "L", "go_ids": [ "GO:0005737", "GO:0032299", "GO:0000287", "GO:0003723", "GO:0004523", "GO:0043137", "GO:0006298" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQ93", "EC:3.1.26.4", "UniRef:UniRef50_Q9PQ93", "COG:COG1039", "COG:L", "GO:0005737", "GO:0032299", "GO:0000287", "GO:0003723", "GO:0004523", "GO:0043137", "GO:0006298" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQ93", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQ93" ], "product": "Ribonuclease HIII" } }, "id": "NKIECMJPJD_797", "locus": "GCA000828735_00784" }, { "type": "cds", "sequence": "contig_1", "start": 466819, "stop": 467001, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTTTAAAAATGATTGCAATTATTGAAGAACAATTTAACGTTAAAATCCCTTTAGGAAGTTCAAATCCTTTAGTTAATGACGTCGCTAGTAATATTTTTCAAAAATTTGGCTTAAAAATTTTAAAACAAGTCGCTAAAGAACACTTTAGTAACTTTCAACAAATTATTAAAAATAAATAA", "aa": "MFLKMIAIIEEQFNVKIPLGSSNPLVNDVASNIFQKFGLKILKQVAKEHFSNFQQIIKNK", "aa_hexdigest": "ec0198ac00232ad2a49ef8d5f54eb74e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6891.1075, "isoelectric_point": 9.778580665588379 }, "id": "NKIECMJPJD_798", "locus": "GCA000828735_00785" }, { "type": "cds", "sequence": "contig_1", "start": 467150, "stop": 467329, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T107", "UniRef:UniRef90_A0AAC9T107" ], "nt": "ATGTTAGGTCAAACAGTTGTTGGATTCTTAGGAATGATTTCACAATTAGTTTATGCTTTTAACCCTCAACCAAAATTTAATCCTCAACAAATGATCGATAACTCTGTTGCTCGCACTTATGCAAGAATCGCACAAAAACCAATGAATTCAACGATTAGTTTAGGAACTCCATTTTTTTAA", "aa": "MLGQTVVGFLGMISQLVYAFNPQPKFNPQQMIDNSVARTYARIAQKPMNSTISLGTPFF", "aa_hexdigest": "bea4dc0a718c88a5afcf32014c28ca8c", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 467051, "stop": 467343, "observations": { "deletion": [ 467324 ], "mutation": [ 467126 ], "stop": [ 467126 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAC9T107", "query_cov": 1.0, "subject_cov": 0.6082474226804123, "identity": 0.966, "score": 116.0, "evalue": 1.15e-32, "gene_start": 1, "gene_end": 59, "reference_start": 34, "reference_end": 92, "reference_sequence": "MQKISNNFKKVYYGGFSTSNSFNYMWNNALISSMLGQTVVGFLGMISQLVYAFNPQPKFRPQQMIDNSVAHTYARIAQKPMNSTISLGTPFFLICKE" }, "paralog": false, "identity": 0.9587628865979382, "score": 182.0, "evalue": 3.51e-61, "description": "Internal stop codon at 467126. Frameshift due to deletion around 467324. Nonsense mutation around 467126.", "psc": { "uniref90_id": "UniRef90_A0AAC9T107", "uniref50_id": "UniRef50_A0AAC9T107", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAC9T107", "UniRef:UniRef50_A0AAC9T107" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAC9T107", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T107" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 6566.606100000001, "isoelectric_point": 9.991971015930176 }, "id": "NKIECMJPJD_799", "locus": "GCA000828735_00786" }, { "type": "cds", "sequence": "contig_1", "start": 467412, "stop": 467612, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T3G4", "UniRef:UniRef50_A0AAP9ACM2", "UniRef:UniRef90_A0AAC9T3G4" ], "nt": "ATGTTATTTGGGACAATTATTGCTAATACAGCTATTTCTATGATTGGAGGCATAACAAATATTGTAATGGCTAGTTCATCACAACAAAACCACGATTCACAAGATATAGATTCGATTAAAATAGAAACAATTAATGTAGATATGAGTACAATAGTTGGTCCTAAAATTCCCTATATGAATATTTATTATTTAAATTCTTAA", "aa": "MLFGTIIANTAISMIGGITNIVMASSSQQNHDSQDIDSIKIETINVDMSTIVGPKIPYMNIYYLNS", "aa_hexdigest": "312a6d05e095fe282c6a98739d223e4f", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "psc": { "uniref90_id": "UniRef90_A0AAP9ACM2", "query_cov": 1.0, "subject_cov": 0.8918918918918919, "identity": 0.636, "score": 75.9, "evalue": 8.24e-17, "valid": false, "uniref50_id": "UniRef50_A0AAP9ACM2" }, "pscc": { "uniref50_id": "UniRef50_A0AAP9ACM2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAP9ACM2" ] }, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 467331, "stop": 467612, "observations": { "mutation": [ 467403 ], "stop": [ 467403 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAC9T3G4", "query_cov": 1.0, "subject_cov": 0.7096774193548387, "identity": 1.0, "score": 125.0, "evalue": 3.01e-36, "gene_start": 1, "gene_end": 66, "reference_start": 28, "reference_end": 93, "reference_sequence": "MQRIINKNMIYGGFMPLVSGALGFWNKMLFGTIIANTAISMIGGITNIVMASSSQQNHDSQDIDSIKIETINVDMSTIVGPKIPYMNIYYLNS" }, "paralog": false, "identity": 0.989247311827957, "score": 181.0, "evalue": 5.54e-61, "description": "Internal stop codon at 467403. Nonsense mutation around 467403.", "psc": { "uniref90_id": "UniRef90_A0AAC9T3G4", "uniref50_id": "UniRef50_A0AAC9T3G4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAC9T3G4", "UniRef:UniRef50_A0AAC9T3G4" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAC9T3G4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T3G4" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 7195.2036, "isoelectric_point": 4.351502418518066 }, "id": "NKIECMJPJD_800", "locus": "GCA000828735_00787" }, { "type": "cds", "sequence": "contig_1", "start": 467663, "stop": 467938, "strand": "+", "frame": 2, "gene": null, "product": "DivIVA domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T2D9", "UniRef:UniRef90_A0AAC9T2D9" ], "nt": "ATGGTAAATGATAATAAAATTAATGAGTATAAAAATAAAAAATTTGTTGCTAACAAAGTTGGTTACGATCCTGAAAGTGTAGATGTTTTTTTAGACGAATTAATTAATGAATTAGAAATATTTATAAATGAACATAAAAATGCAATATTAAAAATAGAAGAACTTAATGAATATAAAAGAAAATTCGAAAAAGAACGTGAATACACAACATCACTAAATAATTTTATTGATCTTTTAGAAAAAGTTGTTGATAAAAAAAGCACTTTTGATGTTTGA", "aa": "MVNDNKINEYKNKKFVANKVGYDPESVDVFLDELINELEIFINEHKNAILKIEELNEYKRKFEKEREYTTSLNNFIDLLEKVVDKKSTFDV", "aa_hexdigest": "2d237ba36cc1ee24ababcde319dde9e5", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_A0AAC9T2D9", "query_cov": 1.0, "subject_cov": 0.9191919191919192, "identity": 1.0, "score": 174.0, "evalue": 3.06e-55, "valid": true, "product": "DivIVA domain-containing protein", "uniref50_id": "UniRef50_A0AAC9T2D9" }, "pscc": { "uniref50_id": "UniRef50_A0AAC9T2D9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T2D9" ], "product": "DivIVA domain-containing protein" }, "genes": [], "id": "NKIECMJPJD_801", "locus": "GCA000828735_00788" }, { "type": "cds", "sequence": "contig_1", "start": 468141, "stop": 468314, "strand": "+", "frame": 3, "gene": "acpS", "product": "holo-ACP synthase", "db_xrefs": [ "COG:COG0736", "COG:I", "EC:2.7.8.7", "GO:0000287", "GO:0005737", "GO:0006633", "GO:0008897", "SO:0001217", "UniRef:UniRef50_Q9PQ97", "UniRef:UniRef90_Q9PQ97" ], "nt": "ATGAAAGCTTTAAAATTAAAATATGGATATGGAGATATTTTAATTCTGAAAACAGAGAATCAACGCCAAGTTTATCTTAATAAAATTTTAATAAAAGAGTTAGAACTTTCAATTTCATACACAGAAACTTATATAGTTGCTTCTGTAGTAGGTTTAATTAATAATATGAACTAA", "aa": "MKALKLKYGYGDILILKTENQRQVYLNKILIKELELSISYTETYIVASVVGLINNMN", "aa_hexdigest": "721a45fb5f9ccbeabf0f97529595a1fc", "start_type": "ATG", "rbs_motif": "AGxAG", "genes": [ "acpS" ], "truncated": "5-prime", "pseudogene": { "start": 467967, "stop": 468314, "observations": { "mutation": [ 468000 ], "stop": [ 468000 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQ97", "query_cov": 1.0, "subject_cov": 0.4956521739130435, "identity": 1.0, "score": 108.0, "evalue": 1.4e-29, "gene_start": 1, "gene_end": 57, "reference_start": 59, "reference_end": 115, "reference_sequence": "MKLVHGIDIIEWNREELNNPLFAKRILIDNELEYYFQLNSSREKKRYLASVFACKEAVMKALKLKYGYGDILILKTENQRQVYLNKILIKELELSISYTETYIVASVVGLINNMN" }, "paralog": false, "identity": 0.991304347826087, "score": 221.0, "evalue": 1.4e-76, "description": "Internal stop codon at 468000. Nonsense mutation around 468000.", "psc": { "uniref90_id": "UniRef90_Q9PQ97", "gene": "acpS", "product": "holo-ACP synthase", "ec_ids": [ "2.7.8.7" ], "uniref50_id": "UniRef50_Q9PQ97", "cog_id": "COG0736", "cog_category": "I", "go_ids": [ "GO:0005737", "GO:0008897", "GO:0000287", "GO:0006633" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQ97", "EC:2.7.8.7", "UniRef:UniRef50_Q9PQ97", "COG:COG0736", "COG:I", "GO:0005737", "GO:0008897", "GO:0000287", "GO:0006633" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQ97", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQ97" ], "product": "Holo-[acyl-carrier-protein] synthase" } }, "id": "NKIECMJPJD_802", "locus": "GCA000828735_00789" }, { "type": "cds", "sequence": "contig_1", "start": 468419, "stop": 468553, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAGTAGTTAATAACAAAACTTTATATCGTTACCAACTACAAAAAAGTAATAATTATGTTTTTCAAACAGCAAAAGAAGTTTATTCTATTAAACGTGCTGATCAAATTGTTAATAAAAATTATTATTGA", "aa": "MKKVVNNKTLYRYQLQKSNNYVFQTAKEVYSIKRADQIVNKNYY", "aa_hexdigest": "c858ba5c6254e0a6b1155aa46a55a905", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5393.139200000002, "isoelectric_point": 9.93091945648193 }, "id": "NKIECMJPJD_803", "locus": "GCA000828735_00790" }, { "type": "cds", "sequence": "contig_1", "start": 469307, "stop": 469603, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTATTCCAATTAGTTGTTTTCTCCCCTTTTTAATTGCTGGTGGTGTTGCTAATTTGTATTCAACATCTAAAATTAATCAAGTAGCCAAATACATGAATATTAATTATGATAAATATGATAGTAATACAATTTTAGATGTAGAAAAATTTTATTTAAATAATCATAATGATGAAGTTTATTTAATCACTAAAAATTTAAATAATCCACAATTTAGTAAACGTCAGAATTATTTTTTGAAAGCAGCGTTTGATCACGCAAAAAATGCATCGACATTTTTTCATGTCTTATCTTGA", "aa": "MIIPISCFLPFLIAGGVANLYSTSKINQVAKYMNINYDKYDSNTILDVEKFYLNNHNDEVYLITKNLNNPQFSKRQNYFLKAAFDHAKNASTFFHVLS", "aa_hexdigest": "abe39ac1ebc38427526e3fea5375d7d4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11333.805799999987, "isoelectric_point": 8.688291358947755 }, "id": "NKIECMJPJD_804", "locus": "GCA000828735_00791" }, { "type": "cds", "sequence": "contig_1", "start": 469772, "stop": 470074, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTACTTTTTAAAAAAAGCACATATTTTTTAGTAGGAATTAGCTTATTTGTTAATTTAATTTTTATAATTATAAATGGTTTAAATATTGCTAATAATATAAAAACGTATTTGATTTTATTTTATCTTTTTACTAGTATTAATTCACTTTTAACAATAGTCTTTAGCACTATTAAAGCTATTAATTTATTCAAAGATTTAAAGGATGAAGGAATTGAAATTTTAGTCTTTTCAAAAAGTATTTCACGTAAAAACATTATTTTTACTAAAATAGGTTTTTTAATATTAAATATTGTTTTTTGA", "aa": "MVLFKKSTYFLVGISLFVNLIFIIINGLNIANNIKTYLILFYLFTSINSLLTIVFSTIKAINLFKDLKDEGIEILVFSKSISRKNIIFTKIGFLILNIVF", "aa_hexdigest": "0adbe596c64db1ad88c5a42c1bee9979", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11458.783899999984, "isoelectric_point": 9.853041648864746 }, "id": "NKIECMJPJD_805", "locus": "GCA000828735_00792" }, { "type": "cds", "sequence": "contig_1", "start": 470399, "stop": 471685, "strand": "-", "frame": 2, "gene": "clpA", "product": "ATP-dependent serine proteinase-heat shock protein", "db_xrefs": [ "COG:COG0542", "COG:O", "SO:0001217", "UniRef:UniRef50_V5RI22", "UniRef:UniRef90_Q9PQ99" ], "nt": "TTGCCAGATAAAGCTATTGATTTAATTGATGAAGCAGCTGCAAAGATTAAAACGCAAATTAATTCACAACCAATATATTTAGATGAAATTAAACGTGATTTACAACATTTACAAACTGAAAAAGCCGCTTTAGAACCAGAAAAAGATGAAAAATCAATTAAACGTTTAAATGAAATTTTAGAAAAAATTAAATTAAAACAAGATGAATTTAACAATTTAAATGATTTGTATTTAAAAGAAAAAAAGCAAATTGACGATTTAAAAAATTTACGGCAAAAAATTGAAAGAATTCAACATGATATTGAATTTTATCAATCTGAGGGTAAGTATGAAAAAGCATCAAGATTACTTTATTCAGATTTACCATTATTTGAAAAACAACGAGAAGAATTAGAAAATAAAATTAATGAGAATAATAATCAAAAAATGATTATTGATTCATTAACAGAGAATGAAATTGCTGATGTTATTGCGCGTGCTACAGGAATTCCATTAAGTCATTTATTAGCTGATGAAAAAACAAAATTATTATCGCTAAACAAACGAATTGCTAGTCATGTTATTGGTCAAGATGAAGCAGTTAAATTAATAAGTGATGCGGTAATCCGTGGTCGTGCAGGTATTAATAATCCTAACCAACCTATTGGTTCTTTCTTATTTTTAGGACCTACGGGCGTTGGTAAAACAGAATTAGCAAAAACTTTAGCAAAAGAATTGTTCAATTCAGAAAAAGCCTTAATTAGATTTGATATGAGTGAATATATGGAAAAACATAGTGTATCAAAATTAGTTGGTGCTCCACCTGGTTATATTGGTTATGAAAATGCTGGAGTTTTAACTGAATCTGTTAAACGCAAACCATATAGTATTTTGTTATTTGATGAAATTGAAAAAGCACACCCTGATATTTTAAATATTTTATTACAAATATTGGATGAAGGTAGCATTAAGGATGCTAAAAATAATGAAATTAATTTTAAAAATACAATTATTATTATGACATCTAATATTGGAGCAGAAGCTTTATTAGAAAATAATAAAACAAAGGCATTACTTGATTTACAAAAAACTTTTAAACCAGAAATTTTAAACCGAATTTCTGAAATTATTTTCTTTAATAAGTTATCAAAAGAAATCATTTTTAAGATTAGTGAAAATTTACTTAAAGAATTAGAAAATTTATTAGCTAAGCAAGATTATTTAATTAAATTTGATGATAATATTGCTCAAATCATGGTTGAAGATGCTTATAGTTCAAATTATGGTGCAAGACCTTTAAAACGATGA", "aa": "MPDKAIDLIDEAAAKIKTQINSQPIYLDEIKRDLQHLQTEKAALEPEKDEKSIKRLNEILEKIKLKQDEFNNLNDLYLKEKKQIDDLKNLRQKIERIQHDIEFYQSEGKYEKASRLLYSDLPLFEKQREELENKINENNNQKMIIDSLTENEIADVIARATGIPLSHLLADEKTKLLSLNKRIASHVIGQDEAVKLISDAVIRGRAGINNPNQPIGSFLFLGPTGVGKTELAKTLAKELFNSEKALIRFDMSEYMEKHSVSKLVGAPPGYIGYENAGVLTESVKRKPYSILLFDEIEKAHPDILNILLQILDEGSIKDAKNNEINFKNTIIIMTSNIGAEALLENNKTKALLDLQKTFKPEILNRISEIIFFNKLSKEIIFKISENLLKELENLLAKQDYLIKFDDNIAQIMVEDAYSSNYGARPLKR", "aa_hexdigest": "d16e9756dbebe911d55f71031d542c3f", "start_type": "TTG", "rbs_motif": null, "genes": [ "clpA" ], "truncated": "both", "pseudogene": { "start": 470282, "stop": 471985, "observations": { "mutation": [ 470401, 471772 ], "stop": [ 470401, 471772 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQ99", "query_cov": 1.0, "subject_cov": 0.6079545454545454, "identity": 0.897, "score": 725.0, "evalue": 7.58e-258, "gene_start": 1, "gene_end": 428, "reference_start": 238, "reference_end": 665, "reference_sequence": "MEFKQKNTSNPLEEFGRNLNQEILDNKIDPIIGRDEEIRRTIEILSRKTKNNPVLIGEPGVGKTAIVEGLAYRIVHKDVPSNLLDKTIIELSLSSLIAGASYQGQFEERINAILKEVKKSNGNIILFIDEIHQIVGMGRNQGSNMDVANILKPMMARGEIKLIGATTLDEYRLYIEKDQALERRFTKVLVNKPTIQETLTIMRGLKPRWEAFHGIKIHDNALIAAVNLSERYINDRNLPDKAIDLIDEAAAKIKTQINSQPIYLDEIKRDLQHLQTEKAALESEKDEKSIKRLNDILEKIKLKQDEFNNLNDIYLKEKKQIDDLKNLRQKIERIQHDIEFYQSEGKYEKASRLLYSDLPALEKQRGELENKINENSSQKMIVDSLTENEIADVIARATGIPLNHLLADEKTKLLSLNKRIAAHVIGQDEAVKLISDAVIRGRAGINNPNQPIGSFLFLGPTGVGKTELAKTLAKELFDSQKALIRFDMSEYMEKHSVSKLVGAPPGYVGYENAGLLTESVKRKPYSILLFDEIEKAHPDVLNILLQILDEGSVKDAKNNEINFKNTIIIMTSNVGAEALLENNKTNALMELQKTFKPEILNRISEIIFFNKLSKEVIFKISENLLKELSDLLAKQDYSIKFNDNIAKIMVDEAYSSNYGARPLKRWITKHLENEIAKLIIENRITKNRNYEINYDREADQVLIK" }, "paralog": false, "identity": 0.9153439153439153, "score": 1006.0, "evalue": 0.0, "description": "Internal stop codon at 470401, 471772. Nonsense mutation around 470401, 471772.", "psc": { "uniref90_id": "UniRef90_Q9PQ99", "gene": "ClpA", "product": "ATP-dependent serine proteinase-heat shock protein", "uniref50_id": "UniRef50_V5RI22", "cog_id": "COG0542", "cog_category": "O", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQ99", "UniRef:UniRef50_V5RI22", "COG:COG0542", "COG:O" ] }, "pscc": { "uniref50_id": "UniRef50_V5RI22", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_V5RI22" ], "product": "ATP-dependent Clp protease, ATPase subunit ClpB" } }, "id": "NKIECMJPJD_806", "locus": "GCA000828735_00793" }, { "type": "cds", "sequence": "contig_1", "start": 471770, "stop": 472396, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00004.35", "PFAM:PF13191.12", "PFAM:PF07728.20", "PFAM:PF05621.17" ], "nt": "ATGGAATTTAAACAAAAAAACTCAAACAATCCATTAGAGGAATTTGGACGTAATTTAAATAAGGAAATTTTAGATAATAAAATTGACCCCATTATCGGTCGAGATGAAGAAATTAGAAGAACCATTGAAATTCTAAGTCGTAAAACTAAGAATAATCCTGTCTTAATTGGTGAACCTGGTGTTGGTAAAACAGCAATTGTTGAGGGGCTTGCATACCGAATTGTTCATAAAGATGTTCCTTCAAACTTATTAGATAAAACAATTATTGAATTATCATTATCATCTTTAATTGCAGGCGCTAGTTATCAAGGCCAGTTTGAGGAACGAATTAATAGTATTTTAAAAGAAGTCAAAAAAGCAGATGGTAATATCATTTTGTTTATTGATGAAATACATCAAATTGTTGGTATGGGTAGAAATCAAGGTTCAAATATGGATGTTGCTAATATTTTAAAACCAATGATGGCAAGAGGAGAAATCAAATTAATTGGCGCCACAACGCTTGATGAGTATCGTTTATATATTGAAAAAGATCAGGCATTAGAACGTCGTTTTACAAAAGTTTTAGTTAACAAACCAACAATTCAAGAAACTTTAACAATAATGCGAGGTTTAAAGCCACGGTGA", "aa": "MEFKQKNSNNPLEEFGRNLNKEILDNKIDPIIGRDEEIRRTIEILSRKTKNNPVLIGEPGVGKTAIVEGLAYRIVHKDVPSNLLDKTIIELSLSSLIAGASYQGQFEERINSILKEVKKADGNIILFIDEIHQIVGMGRNQGSNMDVANILKPMMARGEIKLIGATTLDEYRLYIEKDQALERRFTKVLVNKPTIQETLTIMRGLKPR", "aa_hexdigest": "f0b7490c62b8d24956fbc219301f0976", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "psc": { "uniref90_id": "UniRef90_A0A2X1PP87", "query_cov": 0.9663461538461539, "subject_cov": 0.8375, "identity": 0.5870000000000001, "score": 243.0, "evalue": 1.76e-78, "valid": false, "product": "Protein disaggregation chaperone", "uniref50_id": "UniRef50_A0A453E693" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00004.35", "name": "AAA", "length": 141, "aa_cov": 0.6394230769230769, "hmm_cov": 0.8723404255319149, "evalue": 3.01465235440208e-18, "score": 62.11042022705078, "start": 54, "stop": 186 }, { "id": "PF13191.12", "name": "AAA_16", "length": 51, "aa_cov": 0.23076923076923078, "hmm_cov": 1.0, "evalue": 2.8847787868248422e-09, "score": 33.20856857299805, "start": 31, "stop": 78 }, { "id": "PF07728.20", "name": "AAA_5", "length": 81, "aa_cov": 0.38461538461538464, "hmm_cov": 0.9259259259259259, "evalue": 1.9185877893449065e-06, "score": 23.49312400817871, "start": 54, "stop": 133 }, { "id": "PF05621.17", "name": "TniB", "length": 152, "aa_cov": 0.6634615384615384, "hmm_cov": 0.9671052631578947, "evalue": 5.4314186249038856e-09, "score": 31.295740127563477, "start": 53, "stop": 190 } ], "seq_stats": { "molecular_weight": 23551.060400000006, "isoelectric_point": 8.99960918426514 }, "id": "NKIECMJPJD_807", "locus": "GCA000828735_00794" }, { "type": "cds", "sequence": "contig_1", "start": 472692, "stop": 472994, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTATCTATTGGATGGTATTACAATGTGTTTTATGATTAGTTATGTAGCGGCGATTGTAGGATTAGATTTGACGTTAATAGCCATTGGAATTAGTTTATCAATCATTGTCGCAATGGGAATACTAGGTTTTATTATGAATCATAAAATTGCTTTTAAATTAGGCATTGCTAATATCGTTTTATTTACTATTGCTACAATTGGTTCAATTATTTTATTGATTGTATTTTTTACAACGAGATATAACCATACTGGTTTTAACAAAGGTGTTATTATTGCTGACATTGTATTAAGTATTATTTGA", "aa": "MYLLDGITMCFMISYVAAIVGLDLTLIAIGISLSIIVAMGILGFIMNHKIAFKLGIANIVLFTIATIGSIILLIVFFTTRYNHTGFNKGVIIADIVLSII", "aa_hexdigest": "dd189659146490fa8df15f202963e56e", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10793.159899999984, "isoelectric_point": 7.927435493469239 }, "id": "NKIECMJPJD_808", "locus": "GCA000828735_00795" }, { "type": "cds", "sequence": "contig_1", "start": 473004, "stop": 473318, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATTTAAAGTCATTTGAATTTTTAAAAGGTGATGCAATTGTTGATAACCAAATAAAAAAAGCTATTAATACTAGTTTGATTAAAGCATCATTTTCATGTGCAATTATGTGTATATTAGTTCTTATTTTTGGTTTTTTAATGCGAAAATATTTATTTTATTTTTCAGATGCATCTAGTTTAATTATTAGCTCTCGTTTGATTTTTCAAATAATTACAATCATTGTTGCTACTTTATCAATTGTCTATTTTATTGTTCATTTAATCATTTACTTTAGACAAAGAAATAATTTTTTAGCTCCTAAATTTGCATGA", "aa": "MNLKSFEFLKGDAIVDNQIKKAINTSLIKASFSCAIMCILVLIFGFLMRKYLFYFSDASSLIISSRLIFQIITIIVATLSIVYFIVHLIIYFRQRNNFLAPKFA", "aa_hexdigest": "0854ca84e862b1aaec9a068c48696db5", "start_type": "TTG", "rbs_motif": "GGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11969.341199999983, "isoelectric_point": 9.799210548400879 }, "id": "NKIECMJPJD_809", "locus": "GCA000828735_00796" }, { "type": "cds", "sequence": "contig_1", "start": 473379, "stop": 473474, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATCGTGGTTATAAAACAATCGTTATTAATGATTATGAAATTTGTTTAAACTATAATCAAGTTAAAAATAATATTATTAAATTAATTGATTAA", "aa": "MDRGYKTIVINDYEICLNYNQVKNNIIKLID", "aa_hexdigest": "3c66fbb2fa8074782631b0e3ef4e13c6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3717.2728000000016, "isoelectric_point": 6.006028556823729 }, "id": "NKIECMJPJD_810", "locus": "GCA000828735_00797" }, { "type": "cds", "sequence": "contig_1", "start": 473520, "stop": 474596, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13087.12" ], "nt": "ATGTTACCATTAATAAGTAAGAGTTTTGATTATGGAATTTTTGATGAGGCTTCTCAGATGTTTTTAGAACGTTCTTATCCTTCAATCTATCGTTGTAATATTAATATTGTTGCAGGCGATGATAAACAATTATGCCCTACTAATTTTTTTATTAATCGTCAAGAAAATGAAAACGATGAGTTTGAATTAGCAGATGTAGATGTTGCAGAAAGTTTATTAGATCGTGCTAAAACTGCTCTATGGTCAGAATATTTATTAAAAAACCACTATCGTTCTAATCGAGAAGATTTAATTAGTTTTAGTAACGATAATATTTATAATGGCGATTTACATTTTGCATCTAAAAATGGGTTATTTGATCCAGGAATGGAAATTATAGATGTTGAAGGTGTATTTGAAAATGAAAATGTTATTGAAGCACAAAAAATTATGGAGGTTTTAAAAGAACGAGCAGCCAATTATCAAAAAATTATTGTTATAACTTTTAATATCAAACAAAGTGAATTAATTGAAAATTTAATACTACAAACTTTTAGTTTTAATGATGTTGTTTATCAACGTTTTGAGAATGATGAAATTATTGTTACTAATTTAGAAAATGTTCAAGGTAATGAAGCTGATTTAGTAATTTTATCGGTTACTTATGCCAAAAATAAAGAAGGAATTTTACGCAATAATTATGGCCCATTAATGAAAAAAGGTGGTGCTAATCGGTTAAATGTAGCGATTACAAGAGCAGCAGATAAAATGATTGTTGTTAAATCAATTAAAGCTGTGGATATGATGGTTAATATTAATAATGATAATATTAGGACTTTTAAAAATTTTATTGGTTATTGTGATGAAATTGTTCATTTGACTAATCAAAATAAATTTAATGATTGTAGTCATTTTGATGAAGAATTTTTAACAAGTTTAGAAAAAACCATATTGAAAATTTTAAAAGATTATAAAAAAACAAATCAACAAATTTTATATAAACTAAAAATTGGTAATTTTAAAATCACTTTTGCTTTATATAATGAAGAAAAACAAAAAATCGATTTACTTATTTTTATAGAAGAGTTTAATGAATGA", "aa": "MLPLISKSFDYGIFDEASQMFLERSYPSIYRCNINIVAGDDKQLCPTNFFINRQENENDEFELADVDVAESLLDRAKTALWSEYLLKNHYRSNREDLISFSNDNIYNGDLHFASKNGLFDPGMEIIDVEGVFENENVIEAQKIMEVLKERAANYQKIIVITFNIKQSELIENLILQTFSFNDVVYQRFENDEIIVTNLENVQGNEADLVILSVTYAKNKEGILRNNYGPLMKKGGANRLNVAITRAADKMIVVKSIKAVDMMVNINNDNIRTFKNFIGYCDEIVHLTNQNKFNDCSHFDEEFLTSLEKTILKILKDYKKTNQQILYKLKIGNFKITFALYNEEKQKIDLLIFIEEFNE", "aa_hexdigest": "b317551be91cddb801aa89ff48150ccc", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13087.12", "name": "AAA_12", "length": 209, "aa_cov": 0.5167597765363129, "hmm_cov": 0.9090909090909091, "evalue": 3.0887113853176154e-18, "score": 61.598480224609375, "start": 69, "stop": 253 } ], "seq_stats": { "molecular_weight": 41561.78549999999, "isoelectric_point": 4.874762153625488 }, "id": "NKIECMJPJD_811", "locus": "GCA000828735_00798" }, { "type": "cds", "sequence": "contig_1", "start": 474624, "stop": 474851, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTGTAAAAAATTAAGAGATGCAAATATTCAATTTTCTAAAAATGCTGATGAAATTATTTACAATAACTATTTAGATTATTTGCAAATTAAATTAGTTACTGCTCCTAAACAAATCCAAACAAAAATTAAAGAAATTGCGCAAATTTGTCGTTTAGCAAAAAAACCTAATATTGTGAAATTTATTTTAAAATATTATGAAATTTTACGTTTTCTTTTCCCTATTTGA", "aa": "MCKKLRDANIQFSKNADEIIYNNYLDYLQIKLVTAPKQIQTKIKEIAQICRLAKKPNIVKFILKYYEILRFLFPI", "aa_hexdigest": "d7022c60d316f8b67be0c9762e3ad73f", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8979.708099999996, "isoelectric_point": 9.697543907165528 }, "id": "NKIECMJPJD_812", "locus": "GCA000828735_00799" }, { "type": "cds", "sequence": "contig_1", "start": 475275, "stop": 475721, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATCAATGAAATTAATAATTTAATTGAGAATTATTCTTATAATTTTGATGAAATTATTACACACGTTAAAGCTAGTTATGATTTTTTTAAAAAATATAATTATGAATTTTCTAAAAATAATTTTTTAAAATTTAAAGAAGAAAGCGAACAATTAAATAAATTACAAATTGATTTTAATTTAATTACAAAATCTTTATATCAATCACAAACTTTTCTACCACGTATTAAGTTGTTAGAAAGCTTTTTAGTTAAAAATAATATGACTTTAACAAGTCTTATATATGATGATGTTAACTTGTTAAGTAATACATATGTTGAAGCTAATGAGTTTTATACAAACTTTAATCATCTTTTAAAAGATGATTTTAAAAAATTTATTGAAGATAATATAAAAAGTCATTCATTGTTTTTTAGTCATTTAAATACTTTAAAAAATAATGAATGA", "aa": "MINEINNLIENYSYNFDEIITHVKASYDFFKKYNYEFSKNNFLKFKEESEQLNKLQIDFNLITKSLYQSQTFLPRIKLLESFLVKNNMTLTSLIYDDVNLLSNTYVEANEFYTNFNHLLKDDFKKFIEDNIKSHSLFFSHLNTLKNNE", "aa_hexdigest": "0b028ebe6cbbd9864b272b9a0a43f5ce", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17846.920899999997, "isoelectric_point": 5.582180213928221 }, "id": "NKIECMJPJD_813", "locus": "GCA000828735_00800" }, { "type": "cds", "sequence": "contig_1", "start": 475770, "stop": 476762, "strand": "-", "frame": 1, "gene": null, "product": "DUF4011 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00242D11F6", "UniRef:UniRef90_UPI00242D11F6" ], "nt": "TTGATTGGTACAACACCTAAAGCTTCAAATATTAACGCGCCCTTAATTTTTGTTGATGTTGAGATTAAAAAATATAATGGTGAAATTTTTTTAAAACGAAAAAATACTAATTTTATTGTTAACGAAAAAATACTATCTTTTTTAAAAAATGATTATAAAAATAATATCGTTTCTTTAAATGAAATTAAAAACTTTAGTTATTTAGAAATTCAAACTCTAATTAAAAAAATAATACTAAAAGATCATTTAGAATCGATTATTAATAATAATGAATTTCTAAACTTAAAACAAAATGAAATTAATGAGCAATTTAATAACTTTTTTTTATATGATGCATATGTTTTAGGAATTTTTGAGCCCCATGGAGGAGCGATTAAAAATGACTTAGAAGTGATTATTAGTAATAATATTGATCCTTTTGACGAAGAAATAAATAATGTTAATCAACAAATTTATTATAAAAACTATCATTTTTTAGCAGAAGAAAATAAGCTTCCTTTAATCCAAATTAATCGTCCTTTAAATATTTTTCAAAAGTATGCTATTCATTCAGGATTAGTTAAAAATACTTTAATATATGGACCGCCTGGAACGGGAAAATCAGAGGTGATTGCAAGTATTATTGCAAACGTAATTAATGATTTAAAATCGATTTTAATTATTTCGGAGAAAAAAGCTGCTTTAGATGTATTACATGAAAGATTAGCTAAAATTAATTTTTTCACACTTTTTATTTATGATACAAATAATGATGATCAATTTTATAATAACATTTTTAATTTAATTGAAAAAATAGGAGGATTGCGTTTTATTGAGAATCAAGATTTAAACCAATTCAATGATTTTAAATATAAAAATAATATTATTTCAAACATTTATTATAAAAAGATTCATGAATTAAAAAAAGAAATTATTAATATTACATCTTTAATTAAAAATTTACATAATGAAAAAGATAATAATGATAATAATTTTGCTAATTATTTGTATTGA", "aa": "MIGTTPKASNINAPLIFVDVEIKKYNGEIFLKRKNTNFIVNEKILSFLKNDYKNNIVSLNEIKNFSYLEIQTLIKKIILKDHLESIINNNEFLNLKQNEINEQFNNFFLYDAYVLGIFEPHGGAIKNDLEVIISNNIDPFDEEINNVNQQIYYKNYHFLAEENKLPLIQINRPLNIFQKYAIHSGLVKNTLIYGPPGTGKSEVIASIIANVINDLKSILIISEKKAALDVLHERLAKINFFTLFIYDTNNDDQFYNNIFNLIEKIGGLRFIENQDLNQFNDFKYKNNIISNIYYKKIHELKKEIINITSLIKNLHNEKDNNDNNFANYLY", "aa_hexdigest": "fc39b2fd1ba2499628a7d157ba950fcf", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 475836, "stop": 477062, "observations": { "mutation": [ 476789 ], "stop": [ 476789 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00242D11F6", "query_cov": 0.9333333333333333, "subject_cov": 0.6623655913978495, "identity": 0.99, "score": 593.0, "evalue": 3.23e-211, "gene_start": 1, "gene_end": 308, "reference_start": 158, "reference_end": 465, "reference_sequence": "MQDQGVNNKAIKQKVLNLLSTDTRDSCIRTRLTKYHLDISHTFNKYELQDFIEGKINILKIRNFININTFENDVKNINSMDELIDYLQDFNLKLKDLLSTRKINEWSQDFVFYKNERIADMIKAIESQPKKFEYLSREARNIYAQTGVWPLYIAKNFLIGTTPKASNINAPLIFVDVEIKKYNGEIFLKRKNTNFIVNEKILSFLKNDYKNNIVSLNEIKNFSYLEIQTLIKKIILKDHLESIINNNEFLNLKQNEINEQFNNFFLYDAYVLGIFEPHGGAIKNDLEVIISNNIDPFDEEINNVNQQIYYKNYHFLAEENKLPLIQINRPLNIFQKYAIHSGLVKNTLIYGPPGTGKSEVIASIIANVINDLKSILIISEKKAALDVLHERLAKINFFTLFIYDTNNDDQFYNNIFNLIEKIGGLRFIENQDLNQFNDFKYKNNIISNIYYKRIHELKKEIIDIT" }, "paralog": false, "identity": 0.9926470588235294, "score": 804.0, "evalue": 8.61e-297, "description": "Internal stop codon at 476789. Nonsense mutation around 476789.", "psc": { "uniref90_id": "UniRef90_UPI00242D11F6", "product": "DUF4011 domain-containing protein", "uniref50_id": "UniRef50_UPI00242D11F6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00242D11F6", "UniRef:UniRef50_UPI00242D11F6" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00242D11F6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00242D11F6" ], "product": "DUF4011 domain-containing protein" } }, "id": "NKIECMJPJD_814", "locus": "GCA000828735_00801" }, { "type": "cds", "sequence": "contig_1", "start": 476787, "stop": 477233, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAGATCAAGGAGTAAATAACAAGGCAATTAAGCAAAAAGTCTTAAATTTACTATCAACTGATACGCGTGATAGTTGTATAAGAACACGCTTAACAAAATACCATTTAGATATTAGTCACACTTTTAATAAATATGAACTTCAAGATTTTATTGAAGGTAAAATTAACATTCTTAAAATTAGAAATTTTATTAACATCAATACGTTTGAAAATGATGTTAAAAATATAAATTCAATGGATGAATTGATTGATTATTTACAGGATTTCAATTTAAAACTAAAAGATCTTTTATCGACACGTAAAATTAATGAATGGAGCCAAGACTTTGTATTTTATAAAAATGAACGAATTGCTGATATGATTAAAGCGATTGAAAGTCAACCAAAAAAATTTGAATATTTAAGTCGTGAAGCACGCAATATTTATGCGCAAACAGGTGTTTGA", "aa": "MQDQGVNNKAIKQKVLNLLSTDTRDSCIRTRLTKYHLDISHTFNKYELQDFIEGKINILKIRNFININTFENDVKNINSMDELIDYLQDFNLKLKDLLSTRKINEWSQDFVFYKNERIADMIKAIESQPKKFEYLSREARNIYAQTGV", "aa_hexdigest": "bef0e5f63cb1ac873d906a4b20ba3128", "start_type": "ATG", "rbs_motif": "AGGAG(G)/GGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17602.872999999985, "isoelectric_point": 8.572828483581542 }, "id": "NKIECMJPJD_815", "locus": "GCA000828735_00802" }, { "type": "cds", "sequence": "contig_1", "start": 477238, "stop": 477480, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTAATTACTAATAGTTTCAATGATAACGTAGGTGCATTTGCTAAATCATTAAAATTAAAAAGTATTAAAAAAGTAGTGGGATTTTTAGATGTTGCGGCTTTTTTAACGGCGTTTACAAACGATAAATTTCATGTTTTAAATGGTAATTTACACATCAAAATTTTACAAGCAATTAAAAAAGCAAAATTCAAAGGCGTTGTTTTAGAACATATTAATAATTTATTAGGAGTTGAAGATTAA", "aa": "MVITNSFNDNVGAFAKSLKLKSIKKVVGFLDVAAFLTAFTNDKFHVLNGNLHIKILQAIKKAKFKGVVLEHINNLLGVED", "aa_hexdigest": "5aef93447298862e966d05e1a95e965a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8810.314699999999, "isoelectric_point": 9.757370567321775 }, "id": "NKIECMJPJD_816", "locus": "GCA000828735_00803" }, { "type": "cds", "sequence": "contig_1", "start": 477637, "stop": 478113, "strand": "-", "frame": 3, "gene": "abiF", "product": "Abi family protein", "db_xrefs": [ "COG:COG4823", "COG:V", "SO:0001217", "UniRef:UniRef50_A0AAC9T0D9", "UniRef:UniRef90_A0AAC9T0D9" ], "nt": "ATGGCGTTAAGTAAACACAGTATTGGTTTGATTAAAAATTTAAAGAATAAAGGATTGACTATTAAAAATAAAGAAAAATTCATTAGTTTCTTGTTTAATTATGGAGTCATAAGAGTTATAGATGGTTATTCATTTTTTTTAAAAAAAGATAATCAATATGTAAAAGGCTTAAAAAGTAAGGATTTAATTAATTTTGTTTTATTAGATCGTAAAATTGCTAATTTAATGGGTGATTGTATTATGCTATTTGAACAACAATTAAATGCTATTGTTGTTCAAAATTTTTTAAAGATCAATAATTTAAATTCAAAATATGTTATAGATATGCGAACAGATCCTTTTATAAAATTTGAATCACCAAAACATTATGAAATATTATCAGAAAATATTTACACATATGCTAATTCTTCTAATTTGTTAAGTCAATTTAATACTAACGCACAAGAAATTCCTTTAATTAATTTAGCATTATCATGA", "aa": "MALSKHSIGLIKNLKNKGLTIKNKEKFISFLFNYGVIRVIDGYSFFLKKDNQYVKGLKSKDLINFVLLDRKIANLMGDCIMLFEQQLNAIVVQNFLKINNLNSKYVIDMRTDPFIKFESPKHYEILSENIYTYANSSNLLSQFNTNAQEIPLINLALS", "aa_hexdigest": "90aceaedd8dce4060193e0fae39685e3", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "genes": [ "abiF" ], "truncated": "5-prime", "pseudogene": { "start": 477334, "stop": 478113, "observations": { "mutation": [ 477639 ], "stop": [ 477639 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAC9T0D9", "query_cov": 1.0, "subject_cov": 0.5429553264604811, "identity": 1.0, "score": 306.0, "evalue": 3.17e-103, "gene_start": 1, "gene_end": 158, "reference_start": 1, "reference_end": 158, "reference_sequence": "MALSKHSIGLIKNLKNKGLTIKNKEKFISFLFNYGVIRVIDGYSFFLKKDNQYVKGLKSKDLINFVLLDRKIANLMGDCIMLFEQQLNAIVVQNFLKINNLNSKYVIDMRTDPFIKFESPKHYEILSENIYTYANSSNLLSQFNTNAQEIPLINLALSWTLNTTIGFYRAVDNNVQISVLEYFQLNHITPLEFIDILHIIKHFRNSISHNDMVITNSFNDNVGAFAKSLKLKSIKKVVGFLDVAAFLTAFTNDKFHVLNGNLHIKILQAIKKAKFKGVVLEHINNLLGVED" }, "paralog": false, "identity": 0.9961389961389961, "score": 507.0, "evalue": 5.71e-185, "description": "Internal stop codon at 477639. Nonsense mutation around 477639.", "psc": { "uniref90_id": "UniRef90_A0AAC9T0D9", "gene": "AbiF", "product": "Abi family protein", "uniref50_id": "UniRef50_A0AAC9T0D9", "cog_id": "COG4823", "cog_category": "V", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAC9T0D9", "UniRef:UniRef50_A0AAC9T0D9", "COG:COG4823", "COG:V" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAC9T0D9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T0D9" ], "product": "Abi family protein" } }, "id": "NKIECMJPJD_817", "locus": "GCA000828735_00804" }, { "type": "cds", "sequence": "contig_1", "start": 478209, "stop": 479390, "strand": "-", "frame": 1, "gene": null, "product": "SHS2 domain-containing protein", "db_xrefs": [ "RefSeq:WP_006688645.1", "SO:0001217", "UniParc:UPI00000C1C4A", "UniRef:UniRef100_A0AAC9T230", "UniRef:UniRef50_A0AAP9D7J0", "UniRef:UniRef90_A0AAC9T230" ], "nt": "ATGAACAAACCAATGTGTTTGCTTCGTTTAGATAATTATTATTTTACATTGTTGGTATTTATTTATAATGATGTAACTAAAGTTAATGATTTAATTTTTATAAAATCTATTCCAAACAATAATCAAAATAATTATGAATCATCAAATTTTTTGCAAACAGATTTAAAAATCCAACTTAATACATTGATTAACTGTTTTAAAAAAGAATTGCACATTAATTTTATTGATGAAATTATTGTTAGTTATGGTTTTAGTAGCTTTCAAACTACACATACTACTTTTTATAACTATAATGAATTTATGAAATTAAGATCAAATTTTAATAATGAACACAGTGAATTATTAAGTTCAGATCAAAAAAGTTATATAGCAGTTGATTTTGGAATAAGCCACTATTCTAAAGAAAATAGTTATAAGTTGATTTCATATTATATTTTGAAATATGATTATCAATTAATAATTGATTTGTGCCAATCATTTAATTTAAAAATTAAGCAATTTATAGTTTCACAAGCTCATTTAAACGGTTTAAAATTTGATAATGAAGATAATAAAACTTCATTATTATTAGAATTAGAAAATTTTAAAATAAATTTTAGAACTTATAATGAATATAATTTAATTAGTAATGTTATTAGTGAGACAAAAAACCACTTTTGCCTGGCTGATATCATTACTCAAACAGCACAATCATTAAATATTAATCCTAAAAAGATTGAGAGCTACTTTAATGATATTAATTATAATAATAAAAAAGTTATTGAAATTAATATTGATCCTAAAGAGTTGTTTACTTATAGTTTTATTTGTGGTGCGGATATTGAAAATACTTTTTTAAAAATTTTTAAAGAACATTTTGAAAAAAGTATTTTATCTCAATTTGATCATCCATTACTAGATTTTAATCCAAAGAATTTAGCCTTTCTTTTTATTAACACTCCTTTCAATAATTTAAATAAGATATTTTATTATTTTTTTAGTTCATATTTTAAAGAAGTTAAAATTGAAGTTTTTCAAAATAAATTTTTTATTTATTCAAATTGTTATGAATATAGTAATAGTCAAGCCCTAGCTAAGCAAGATTTTTACAATGTACATAACATGAAAAATTTTGATAGCTTTAGCAATGAAAATATCCTAACAAAGATGCATGATCAAACAATAAACATAAAAAATTTTTAA", "aa": "MNKPMCLLRLDNYYFTLLVFIYNDVTKVNDLIFIKSIPNNNQNNYESSNFLQTDLKIQLNTLINCFKKELHINFIDEIIVSYGFSSFQTTHTTFYNYNEFMKLRSNFNNEHSELLSSDQKSYIAVDFGISHYSKENSYKLISYYILKYDYQLIIDLCQSFNLKIKQFIVSQAHLNGLKFDNEDNKTSLLLELENFKINFRTYNEYNLISNVISETKNHFCLADIITQTAQSLNINPKKIESYFNDINYNNKKVIEINIDPKELFTYSFICGADIENTFLKIFKEHFEKSILSQFDHPLLDFNPKNLAFLFINTPFNNLNKIFYYFFSSYFKEVKIEVFQNKFFIYSNCYEYSNSQALAKQDFYNVHNMKNFDSFSNENILTKMHDQTINIKNF", "aa_hexdigest": "9bcdb318c74dce0b1a678f52b97260b0", "start_type": "ATG", "rbs_motif": "GGAGG", "ups": { "uniparc_id": "UPI00000C1C4A", "ncbi_nrp_id": "WP_006688645.1", "uniref100_id": "UniRef100_A0AAC9T230", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1C4A", "RefSeq:WP_006688645.1", "UniRef:UniRef100_A0AAC9T230" ] }, "ips": { "uniref100_id": "UniRef100_A0AAC9T230", "uniref90_id": "UniRef90_A0AAC9T230" }, "psc": { "uniref90_id": "UniRef90_A0AAC9T230", "uniref50_id": "UniRef50_A0AAP9D7J0" }, "pscc": { "uniref50_id": "UniRef50_A0AAP9D7J0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAP9D7J0" ], "product": "SHS2 domain-containing protein" }, "genes": [], "id": "NKIECMJPJD_818", "locus": "GCA000828735_00805" }, { "type": "cds", "sequence": "contig_1", "start": 479409, "stop": 479960, "strand": "-", "frame": 1, "gene": "rsmH", "product": "16S rRNA (cytosine(1402)-N(4))-methyltransferase RsmH", "db_xrefs": [ "COG:COG0275", "COG:J", "EC:2.1.1.199", "GO:0005737", "GO:0016434", "GO:0070475", "GO:0071424", "SO:0001217", "UniRef:UniRef50_Q9PQA4", "UniRef:UniRef90_Q9PQA4" ], "nt": "ATGCGAATGGATCAATCGCAAAACTTAAATGCTCACTATATTGTTAATAATTATTCTTTTGCAAAATTAGTAAGTATTTTTAAACGATATGGAGAAATAAAATATCCAAAAATTATTGCTGATGCAATTGTTAAAGAACGCAGTATTAAAGAGATTAATACAACATTGGAATTAGTTGAAATCATTAAAAAATATTCTCCTAAAAAAAATTTATTTGAAAAAAAACATCCTGCACGTTTATTTTTTCAAGCAATTAGAATAGAAGTTAATGATGAATTAAACATTTTAGAAAAAGCATTTAATGATGCTATTTCAATGTTAAATCCCTTAGGTGTTGTGGCAATTATTAGCTTTCATAGTCTTGAAGATAAAATTGTTAAAAAGGTCTTTAATAATTATGCAAAAATTAAATTACCTAAAGAAGTCCCAATAAATAATTATGTAAATAAATATAGTTTATTGAATCAAAAAATTATGCCAAGCACCCAGGAACTAAATGATAACAATCGTTCACGATCATCTATTTTAAGGGGATTAATTAGAAATTATTAA", "aa": "MRMDQSQNLNAHYIVNNYSFAKLVSIFKRYGEIKYPKIIADAIVKERSIKEINTTLELVEIIKKYSPKKNLFEKKHPARLFFQAIRIEVNDELNILEKAFNDAISMLNPLGVVAIISFHSLEDKIVKKVFNNYAKIKLPKEVPINNYVNKYSLLNQKIMPSTQELNDNNRSRSSILRGLIRNY", "aa_hexdigest": "9a02f6845db6968554e957312940c3c2", "start_type": "ATG", "rbs_motif": null, "genes": [ "rsmH" ], "truncated": "3-prime", "pseudogene": { "start": 479409, "stop": 480260, "observations": { "mutation": [ 479969 ], "stop": [ 479969 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQA4", "query_cov": 1.0, "subject_cov": 0.5941558441558441, "identity": 1.0, "score": 351.0, "evalue": 2.49e-120, "gene_start": 1, "gene_end": 183, "reference_start": 126, "reference_end": 308, "reference_sequence": "MEFNQHITVLLNETIELLNIKPDGIYVDCTFGRGGHSQLILKKLSKKGKLICLDQDQEAINFANNLFKNNTNVIVIKTNFKNLKSALSAHKIFYVDGFIFDLGLSSPQLDDPKRGFSYHKNAWLDMRMDQSQNLNAHYIVNNYSFAKLVSIFKRYGEIKYPKIIADAIVKERSIKEINTTLELVEIIKKYSPKKNLFEKKHPARLFFQAIRIEVNDELNILEKAFNDAISMLNPLGVVAIISFHSLEDKIVKKVFNNYAKIKLPKEVPINNYVNKYSLLNQKIMPSTQELNDNNRSRSSILRGLIRNY" }, "paralog": false, "identity": 0.9964664310954063, "score": 557.0, "evalue": 4.53e-204, "description": "Internal stop codon at 479969. Nonsense mutation around 479969.", "psc": { "uniref90_id": "UniRef90_Q9PQA4", "gene": "rsmH", "product": "16S rRNA (cytosine(1402)-N(4))-methyltransferase RsmH", "ec_ids": [ "2.1.1.199" ], "uniref50_id": "UniRef50_Q9PQA4", "cog_id": "COG0275", "cog_category": "J", "go_ids": [ "GO:0005737", "GO:0016434", "GO:0070475", "GO:0071424" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQA4", "EC:2.1.1.199", "UniRef:UniRef50_Q9PQA4", "COG:COG0275", "COG:J", "GO:0005737", "GO:0016434", "GO:0070475", "GO:0071424" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQA4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQA4" ], "product": "Ribosomal RNA small subunit methyltransferase H" } }, "id": "NKIECMJPJD_819", "locus": "GCA000828735_00806" }, { "type": "cds", "sequence": "contig_1", "start": 479967, "stop": 480335, "strand": "-", "frame": 1, "gene": "mraW", "product": "MraW methylase family", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D4DTB8" ], "nt": "ATGGAATTTAATCAACACATTACCGTTTTATTAAACGAAACAATCGAATTATTAAATATTAAACCAGATGGCATTTATGTTGATTGTACATTTGGTCGTGGCGGACATTCACAACTCATCTTAAAAAAACTCTCAAAAAAAGGTAAATTAATTTGTTTAGATCAAGATCAAGAAGCAATTAATTTTGCAAATAATTTATTTAAAAATAATACAAATGTAATTGTTATTAAAACAAACTTTAAAAATTTAAAATCAGCATTAAGTGCACATAAAATTTTTTATGTTGATGGCTTTATCTTTGATTTAGGTTTATCAAGTCCACAACTTGATGATCCAAAAAGAGGTTTTAGTTATCATAAGAATGCTTGA", "aa": "MEFNQHITVLLNETIELLNIKPDGIYVDCTFGRGGHSQLILKKLSKKGKLICLDQDQEAINFANNLFKNNTNVIVIKTNFKNLKSALSAHKIFYVDGFIFDLGLSSPQLDDPKRGFSYHKNA", "aa_hexdigest": "c84b2a1e13ca361dba814d2f1a981f61", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_D4DTB8", "query_cov": 0.9672131147540983, "subject_cov": 0.8082191780821918, "identity": 0.5, "score": 119.0, "evalue": 2.59e-32, "valid": false, "gene": "rsmH", "product": "16S rRNA (cytosine(1402)-N(4))-methyltransferase RsmH", "ec_ids": [ "2.1.1.199" ], "uniref50_id": "UniRef50_D4DTB8", "go_ids": [ "GO:0016434" ] }, "pscc": { "uniref50_id": "UniRef50_D4DTB8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D4DTB8" ], "product": "MraW methylase family" }, "genes": [ "mraW" ], "id": "NKIECMJPJD_820", "locus": "GCA000828735_00807" }, { "type": "cds", "sequence": "contig_1", "start": 480325, "stop": 480762, "strand": "-", "frame": 3, "gene": "mraZ", "product": "division/cell wall cluster transcriptional repressor MraZ", "db_xrefs": [ "COG:COG2001", "COG:J", "GO:0000976", "GO:0003700", "GO:0005737", "GO:0006355", "GO:0009295", "GO:2000143", "RefSeq:WP_006688768.1", "SO:0001217", "UniParc:UPI000169E335", "UniRef:UniRef100_A0AAC9X6W2", "UniRef:UniRef50_Q9PQA5", "UniRef:UniRef90_Q9PQA5" ], "nt": "ATGTTTATTGGTACTTATAATCATTCAATTGATTCAAAGAATCGTATGTTAGTTCCATCAAAAGTTAAAGCAACATTAGGTGAAGCCATTTTTGTTTATTTATCACTTGGTTTTGATGGAAATATAGATATGAGATTAGAGAGTGAATTTAATCAATTCGTTAACAATATTAATAATTTATCAATTGGTTCAAAAGAAGCGCGAAATCTTACAAGATTAATATTAAGCCAAACTTATAAAATAGAAATCGATAGTGCGTCAAGAATTTTAATACCTCAAAACCTTATTGATAAAGCTAAAATTAAAAAAGATATTTATATTATTGGAACAAATGATCGATATGAAATTTGGGCTAAAGAAGTTTATGATGATTTTAGCTTAAATCAAGAAAGTACGCTAAGTGATTTAGCTGAAAAATTATTAATTAATGGAATTTAA", "aa": "MFIGTYNHSIDSKNRMLVPSKVKATLGEAIFVYLSLGFDGNIDMRLESEFNQFVNNINNLSIGSKEARNLTRLILSQTYKIEIDSASRILIPQNLIDKAKIKKDIYIIGTNDRYEIWAKEVYDDFSLNQESTLSDLAEKLLINGI", "aa_hexdigest": "9cf7d16b9ab88862a2b7822613ce5a2c", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "ups": { "uniparc_id": "UPI000169E335", "ncbi_nrp_id": "WP_006688768.1", "uniref100_id": "UniRef100_A0AAC9X6W2", "db_xrefs": [ "SO:0001217", "UniParc:UPI000169E335", "RefSeq:WP_006688768.1", "UniRef:UniRef100_A0AAC9X6W2" ] }, "ips": { "uniref100_id": "UniRef100_A0AAC9X6W2", "uniref90_id": "UniRef90_Q9PQA5" }, "psc": { "uniref90_id": "UniRef90_Q9PQA5", "gene": "mraZ", "product": "division/cell wall cluster transcriptional repressor MraZ", "uniref50_id": "UniRef50_Q9PQA5", "cog_id": "COG2001", "cog_category": "J", "go_ids": [ "GO:0000976", "GO:0003700", "GO:0005737", "GO:0006355", "GO:0009295", "GO:2000143" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQA5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQA5" ], "product": "Transcriptional regulator MraZ" }, "genes": [ "mraZ" ], "id": "NKIECMJPJD_821", "locus": "GCA000828735_00808" }, { "type": "cds", "sequence": "contig_1", "start": 480889, "stop": 481032, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTATTCCTCATTTTGAATTAATTTTAGATTTATTTACTAATACATTAGTAAAATCTATTTATTTTATTAGTGAAATTGATGCGAGTATTCTAATTAAAGCTTACGTAAAAATTGTTACGATTTTTTATACGACTATTTGA", "aa": "MFIPHFELILDLFTNTLVKSIYFISEIDASILIKAYVKIVTIFYTTI", "aa_hexdigest": "0f807bfa75b0d4738f38a744bc43ad46", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5499.540899999999, "isoelectric_point": 5.464750862121583 }, "id": "NKIECMJPJD_822", "locus": "GCA000828735_00809" }, { "type": "cds", "sequence": "contig_1", "start": 481036, "stop": 481362, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCATATTTTATCAATTAAATTTTTTCAAAAAATTTCTAAAATGGATAAATTGGCAATTAGCGGAATGTTAATTGGTTGTTTAAGTATTTATAATTTATCGATTTTGAGTTTTATTTTTGCATATTTAGCTTTATTTTGCATTTACCTTATTAATATTTTTATAAAGAACAATTCGGTATTAAAAAATATTTACATAAATTTGTATATTTTATCAATCACTTTTGTCATTTCAATCAATTTAAACCAAAAAATCAATTTTTTAAGTATTGTTTTTGGTTATGTAATGAATTTACCAATTCTTTTTGTATATCAAATACTTTTTTGA", "aa": "MHILSIKFFQKISKMDKLAISGMLIGCLSIYNLSILSFIFAYLALFCIYLINIFIKNNSVLKNIYINLYILSITFVISINLNQKINFLSIVFGYVMNLPILFVYQILF", "aa_hexdigest": "eb7f2dab7e1569cfe694003caaf98452", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12547.236799999982, "isoelectric_point": 9.51774158477783 }, "id": "NKIECMJPJD_823", "locus": "GCA000828735_00810" }, { "type": "cds", "sequence": "contig_1", "start": 481453, "stop": 481737, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTGTTTATTAATTCACAAAGATTTTAATATACATTTTTTAGTTATAAATATCGAAAAAAAATATAATTACTCGTTACGAACACAAATTTTAATATATCTTAGTAAAAAGTACTCAAATGAAAATACTTTAATGTTAATTAAATTAATGATTTTTAATGAAAAGATTAACAGTGATTTTAAGCAAATTTTATATGATCTTAATATCGCTCATTTATTTATTGTTTCAGGATTGCACTTGAATTTATTTATTCTAATCATTAATAAGATTTTTTCTAAACGATGA", "aa": "MCLLIHKDFNIHFLVINIEKKYNYSLRTQILIYLSKKYSNENTLMLIKLMIFNEKINSDFKQILYDLNIAHLFIVSGLHLNLFILIINKIFSKR", "aa_hexdigest": "7675bde361b9632d92c8229e549d8365", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11305.474999999986, "isoelectric_point": 9.58988170623779 }, "id": "NKIECMJPJD_824", "locus": "GCA000828735_00811" }, { "type": "cds", "sequence": "contig_1", "start": 482071, "stop": 482208, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02421.24" ], "nt": "ATGCGAACTATTGCTATTGTCGGAAAGCCAAATGTTGGTAAATCATCATTATTTAATCGTATTTTAATGCGCCGTAAATCAATTGTTGACGATCAGCCAGGAGTAACAAGAGATCGAATATATGATATTGGTAATTGA", "aa": "MRTIAIVGKPNVGKSSLFNRILMRRKSIVDDQPGVTRDRIYDIGN", "aa_hexdigest": "f2b87e50613dbd2af1d693c6f5bc44c1", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_UPI00293EF8CC", "query_cov": 0.9555555555555556, "subject_cov": 0.8113207547169812, "identity": 0.674, "score": 65.5, "evalue": 2.67e-13, "valid": false, "product": "GTPase", "uniref50_id": "UniRef50_A0A533SGT9" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02421.24", "name": "FeoB_N", "length": 37, "aa_cov": 0.8222222222222222, "hmm_cov": 0.972972972972973, "evalue": 1.5116309012052555e-08, "score": 29.869476318359375, "start": 3, "stop": 39 } ], "seq_stats": { "molecular_weight": 5101.910000000001, "isoelectric_point": 10.903167152404784 }, "id": "NKIECMJPJD_825", "locus": "GCA000828735_00812" }, { "type": "cds", "sequence": "contig_1", "start": 482224, "stop": 482955, "strand": "+", "frame": 1, "gene": "der", "product": "ribosome biogenesis GTPase Der", "db_xrefs": [ "COG:COG1160", "COG:J", "EC:3.6.5.-", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0042254", "GO:0043022", "KEGG:K03977", "SO:0001217", "UniRef:UniRef50_P75309", "UniRef:UniRef90_Q9PQA7" ], "nt": "ATGTTGATTGATACAGGAGGTATAATTAGCTCAAAAGATACTTATCAAGATAACATCAATGAACAAGTTTTATTTGCGATTAATGAAGCAAATACAATTATTTTTTTAGTCTCAGCAAAAGATGGAATTAATAATGATGATAAAAAAATAGCTAAAATGTTAAAAGAAAAAGCTAAAGATAAAAAAATTATTTTAGTTATAAATAAGATCGAATCAGAAAAATATTATCTTAATGAAGGAGAACTATATTCGTTTGGGTTTGGTAAATTTTTTAAAATCTCAGCTGAGCATGGAATAGGAATGGGTGATCTTTTAGATGAATTAGTAAAAGATATGCCTATACAAAATAATTTAGAAAAACAAGAACGCTTTAAATTTTGCATTATTGGAAGACCAAATGTTGGTAAAAGTTCACTAACAAATACCATCTTAGGTGAGCAAAGAGTAATTGTTAATGCTGAAGCAGGTTCAACACGTGATTCAATCGATAATGATTTTAATTACTATAATAAAAAATATACAATCATTGACACTGCAGGAATACGACGAAAAGGTAAAATTGTCGAATCTGTTGAAAAATATGCTGTATTACGAACTAAAAAAGCAATTGAAAGATCCCAATTAATTTTATTAGTACTTGATGGATCTGAACCTTTTAAAGAACAAGATGAAGTTGTTGGTGGTTTAGCATATAATGCAAATATTCCAACAATTATTATTGTTAATAAATGA", "aa": "MLIDTGGIISSKDTYQDNINEQVLFAINEANTIIFLVSAKDGINNDDKKIAKMLKEKAKDKKIILVINKIESEKYYLNEGELYSFGFGKFFKISAEHGIGMGDLLDELVKDMPIQNNLEKQERFKFCIIGRPNVGKSSLTNTILGEQRVIVNAEAGSTRDSIDNDFNYYNKKYTIIDTAGIRRKGKIVESVEKYAVLRTKKAIERSQLILLVLDGSEPFKEQDEVVGGLAYNANIPTIIIVNK", "aa_hexdigest": "02a0a1d136977c5a2ca769740a9a05a0", "start_type": "ATG", "rbs_motif": null, "genes": [ "der" ], "truncated": "both", "pseudogene": { "start": 482071, "stop": 483258, "observations": { "mutation": [ 482206, 482953, 483034 ], "stop": [ 482206, 482953, 483034 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQA7", "query_cov": 1.0, "subject_cov": 0.5497737556561086, "identity": 1.0, "score": 467.0, "evalue": 3.35e-163, "gene_start": 1, "gene_end": 243, "reference_start": 52, "reference_end": 294, "reference_sequence": "MRTIAIVGKPNVGKSSLFNRILMRRKSIVDDQPGVTRDRIYDIGNWLTRSFMLIDTGGIISSKDTYQDNINEQVLFAINEANTIIFLVSAKDGINNDDKKIAKMLKEKAKDKKIILVINKIESEKYYLNEGELYSFGFGKFFKISAEHGIGMGDLLDELVKDMPIQNNLEKQERFKFCIIGRPNVGKSSLTNTILGEQRVIVNAEAGSTRDSIDNDFNYYNKKYTIIDTAGIRRKGKIVESVEKYAVLRTKKAIERSQLILLVLDGSEPFKEQDEVVGGLAYNANIPTIIIVNKWDNIINKNSHTMEMVKKQIRSQFKYLSWAPIVFVSALDNKRIHTIFEAIEFVREQAMRKIATSLLNDVVIKANAFQEPPPFKGGRISISYIVQVQSQIPTFVLKCNNPKFLHFSYARYIENEIRKAFGFDSVPITLYWQDKNKKLRGE" }, "paralog": false, "identity": 0.9924050632911392, "score": 756.0, "evalue": 9.06e-280, "description": "Internal stop codon at 482206, 482953, 483034. Nonsense mutation around 482206, 482953, 483034.", "psc": { "uniref90_id": "UniRef90_Q9PQA7", "gene": "der", "product": "ribosome biogenesis GTPase Der", "ec_ids": [ "3.6.5.-" ], "uniref50_id": "UniRef50_P75309", "cog_id": "COG1160", "cog_category": "J", "kegg_orthology_id": "K03977", "go_ids": [ "GO:0003924", "GO:0005525", "GO:0005737", "GO:0042254", "GO:0043022" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQA7", "EC:3.6.5.-", "UniRef:UniRef50_P75309", "COG:COG1160", "COG:J", "KEGG:K03977", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0042254", "GO:0043022" ] }, "pscc": { "uniref50_id": "UniRef50_P75309", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75309" ], "product": "GTPase Der" } }, "id": "NKIECMJPJD_826", "locus": "GCA000828735_00813" }, { "type": "cds", "sequence": "contig_1", "start": 483121, "stop": 483366, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF14714.11" ], "nt": "ATGCGCAAAATTGCAACTTCATTATTAAATGATGTTGTTATTAAAGCAAATGCTTTTCAAGAACCACCACCATTTAAAGGAGGGAGAATCAGTATTTCATATATCGTTCAAGTACAATCACAAATCCCTACTTTTGTTTTAAAATGTAATAACCCTAAATTTTTGCACTTTAGCTATGCTCGATATATTGAAAATGAAATTCGTAAAGCTTTTGGATTTGATAGTGTACCAATAACATTATATTGA", "aa": "MRKIATSLLNDVVIKANAFQEPPPFKGGRISISYIVQVQSQIPTFVLKCNNPKFLHFSYARYIENEIRKAFGFDSVPITLY", "aa_hexdigest": "c2452a01fbd26ed9438cef0d62e8a230", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF14714.11", "name": "KH_dom-like", "length": 78, "aa_cov": 0.9629629629629629, "hmm_cov": 1.0, "evalue": 7.769370298962424e-29, "score": 95.4348373413086, "start": 3, "stop": 80 } ], "seq_stats": { "molecular_weight": 9280.728599999997, "isoelectric_point": 9.664987373352051 }, "id": "NKIECMJPJD_827", "locus": "GCA000828735_00814" }, { "type": "cds", "sequence": "contig_1", "start": 483407, "stop": 483922, "strand": "+", "frame": 2, "gene": "gpsA", "product": "Glycerol-3-phosphate dehydrogenase", "db_xrefs": [ "COG:C", "COG:COG0240", "SO:0001217", "UniRef:UniRef50_Q9PQA8", "UniRef:UniRef90_Q9PQA8" ], "nt": "ATGAGTAAAATTTTAATTGTTGGAAGCGGAGCTTTTGGAAGTGCTTTAACACAAGTTTTAGTTTCAAATTCTCATATTGTTGATGTTTATGGAATTAATGAAAATGAGCTTAATGATTTGAAAGAAAATCAAAAAAATAAGAAATATTTTCAAGATCAAAAATTAGATTTACCAATTAATAATACATATTTGGATATTAATTTAGCACTTCAGAATCAATATGATTTTATCATCATTGCTATTCCTTCATTTGCTATTAAAAACTTTGTTGATAATATCAAAATTTTTAATTTATCAAAAACTATTGTTGTTAATGCAGCGAAAGGATTAAATCTTGAAACAAAATCATCATGGTGTGATTATATTAAACAAAATTTAAAAATTAAAGCCTTAGTTGGATTAGTTGGCCCTTCATTTGCAATTGATGTTTTTTTAAAAAAACCAACAGTCGTTAATTTAGTAGGCAATAATTCAAACGCCCTTATAAAAGTTAAAGAAGAATTTGAAAACAATTGA", "aa": "MSKILIVGSGAFGSALTQVLVSNSHIVDVYGINENELNDLKENQKNKKYFQDQKLDLPINNTYLDINLALQNQYDFIIIAIPSFAIKNFVDNIKIFNLSKTIVVNAAKGLNLETKSSWCDYIKQNLKIKALVGLVGPSFAIDVFLKKPTVVNLVGNNSNALIKVKEEFENN", "aa_hexdigest": "91e3b947e82abdc7a08ffaeb7e3dfc8b", "start_type": "ATG", "rbs_motif": "AGGAG/GGAGG", "genes": [ "gpsA" ], "truncated": "3-prime", "pseudogene": { "start": 483407, "stop": 484225, "observations": { "mutation": [ 483920 ], "stop": [ 483920 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQA8", "query_cov": 1.0, "subject_cov": 0.5277777777777778, "identity": 1.0, "score": 327.0, "evalue": 7.05e-111, "gene_start": 1, "gene_end": 171, "reference_start": 1, "reference_end": 171, "reference_sequence": "MSKILIVGSGAFGSALTQVLVSNSHIVDVYGINENELNDLKENQKNKKYFQDQKLDLPINNTYLDINLALQNQYDFIIIAIPSFAIKNFVDNIKIFNLSKTIVVNAAKGLNLETKSSWCDYIKQNLKIKALVGLVGPSFAIDVFLKKPTVVNLVGNNSNALIKVKEEFENNWFKCILSKQFDAANYISCFKNALAIGCGIIYGLQNSNNSLVAFLTKGINEMQLILEKIFHKKVNPLEYFFIGDTILTCTDQKSRNFSFGLLIAQQGVQEALVNKQQTIEGLNNIKVIYEIIKTNQISAPLFQNLYKVIINALNPQDLFDKSFC" }, "paralog": false, "identity": 0.9963235294117647, "score": 533.0, "evalue": 2e-194, "description": "Internal stop codon at 483920. Nonsense mutation around 483920.", "psc": { "uniref90_id": "UniRef90_Q9PQA8", "gene": "GpsA", "product": "Glycerol-3-phosphate dehydrogenase", "uniref50_id": "UniRef50_Q9PQA8", "cog_id": "COG0240", "cog_category": "C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQA8", "UniRef:UniRef50_Q9PQA8", "COG:COG0240", "COG:C" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQA8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQA8" ], "product": "Glycerol-3-phosphate dehydrogenase" } }, "id": "NKIECMJPJD_828", "locus": "GCA000828735_00815" }, { "type": "cds", "sequence": "contig_1", "start": 484070, "stop": 484381, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF20618.3", "PFAM:PF07479.19" ], "nt": "ATGCAACTAATTTTAGAGAAAATTTTTCACAAAAAAGTCAATCCGTTAGAATACTTTTTTATTGGTGATACAATTTTAACTTGTACTGATCAAAAATCACGTAATTTTAGTTTTGGTTTACTGATTGCACAACAAGGCGTACAAGAAGCTTTAGTAAATAAACAACAGACTATTGAAGGTTTAAATAACATCAAAGTTATTTATGAAATTATTAAAACAAATCAAATTAGTGCTCCACTTTTCCAAAATTTATATAAGGTAATTATTAACGCCTTAAATCCCCAGGATTTATTTGATAAAAGTTTTTGTTAA", "aa": "MQLILEKIFHKKVNPLEYFFIGDTILTCTDQKSRNFSFGLLIAQQGVQEALVNKQQTIEGLNNIKVIYEIIKTNQISAPLFQNLYKVIINALNPQDLFDKSFC", "aa_hexdigest": "002fc72abc73396cfd709e30ac8b49e3", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF20618.3", "name": "GPD_NAD_C_bact", "length": 64, "aa_cov": 0.6116504854368932, "hmm_cov": 0.984375, "evalue": 8.749892792127967e-18, "score": 60.26945114135742, "start": 21, "stop": 83 }, { "id": "PF07479.19", "name": "NAD_Gly3P_dh_C", "length": 97, "aa_cov": 0.9320388349514563, "hmm_cov": 1.0, "evalue": 5.484004130403396e-22, "score": 74.07715606689453, "start": 3, "stop": 98 } ], "seq_stats": { "molecular_weight": 11865.703899999984, "isoelectric_point": 7.74643840789795 }, "id": "NKIECMJPJD_829", "locus": "GCA000828735_00816" }, { "type": "cds", "sequence": "contig_1", "start": 484687, "stop": 486135, "strand": "+", "frame": 1, "gene": "uvrC", "product": "excinuclease ABC subunit UvrC", "db_xrefs": [ "COG:COG0322", "COG:L", "GO:0003677", "GO:0005737", "GO:0006289", "GO:0009380", "GO:0009381", "GO:0009432", "SO:0001217", "UniRef:UniRef50_Q9PQA9", "UniRef:UniRef90_Q9PQA9" ], "nt": "ATGATTACAAATGAACAATATCCCCGTTTAAAATATGTTCGTACTTATGATAAAAATAAAGGAATTTATTTTGGGCCATTAGCTGATAGCACAAATAAATATCAATTATTTAATTTATTAAATAGTATTTTTCCATTTAATAAATGTAATCATCAACCATATAAAAAATGTATTTATTATGACCTGCATCAATGTATTAATCAAGTTCAACAATCAACATATCAAGAAGCAATCAGTGAAGTTAAAGAAATCTTTAAAGGTAATTTAGATCACATTTTAATGATTCTACAAACTAAAGAACAACATGCAGTTACTAAATTAGATTTTGAAAATGCTCAAAAATACGCTGAGCAACAAAAAGCACTTACTTCAATTATTAATTCAGGATTAGTGCAATTGGATAATAATGAAAGTTTCGATATCATCGGTTTTTATGAAAAAAATAATTATTTAGTAATTATAATTTTTAATTATGTTAAAGGCAAATTATTAAATAAAAGTGCTGATACATTTGCCATTTATGATTATGAAATTAATGAATTAATAACCTCATTTTTAATGCAATACTATTCACAAAATAAAATTAGTTCAAAAATTATTGTAAGTTTAGATGATGATAATTTATTAGCTTTATCACAACGTTTTCACACAAAATTTATTAATGCGCAAACAAAATTTCATAAACGAATTCTTAAATTAGCTCTTGATAATGCTATTTTGTATTTTGAATCTAATATTAAAAATGTAATTAATAAACAAAATGAATTAGATGATGCTTTAAATCAATTAAAACAAATTTTAAAATTACCAGATTTAAATATCATTGAGTGTTTTGATAATTCTAATATTAATTTAAGTTTACCAATTGCTGGGATGATTGTTTATCAAAACGGTAAATTAAATAACAAATTAAATCGTAAATATAATTTAATGACAACTAAAAATGCTTCTGATTATCATTTTATGATTGAAGTTATCACACGACGTTATCAACGATTAGTTTCACAACATCAAAAATTACCTAATCTAATTGTAGTAGATGGGGGAAAGTTACAAGTTAATGCAGCTTTATATGCGTTAGCACAGTTACAAATAAACATTCCATTAATAGGACTAAAAAAAGATCAAAAACACAAAACTGATGCCATTGTTTTAACTAATGGCGATGAAATTGTTTTAGATCGTAAAAGTATTTTATATAAATTCTTAGCAAATATGCAAAATGATGTTCACAATTATGCAATTTCGTTTTTAAGAGATAAACACACAAAATCTATTTTTAATTCTTTATTAGATAATGTTCAAGGTCTAGGTAAAAAACGTTTTAATGAACTTTTAAAATATTATGATAGCATTAATGATTTAAAAAGTGCATCTGATCAAGAATTATTACAATTTTTACCTAAAAATGTCTTAGTTAATTTACGTGAAAAACTAAATAAAATATAA", "aa": "MITNEQYPRLKYVRTYDKNKGIYFGPLADSTNKYQLFNLLNSIFPFNKCNHQPYKKCIYYDLHQCINQVQQSTYQEAISEVKEIFKGNLDHILMILQTKEQHAVTKLDFENAQKYAEQQKALTSIINSGLVQLDNNESFDIIGFYEKNNYLVIIIFNYVKGKLLNKSADTFAIYDYEINELITSFLMQYYSQNKISSKIIVSLDDDNLLALSQRFHTKFINAQTKFHKRILKLALDNAILYFESNIKNVINKQNELDDALNQLKQILKLPDLNIIECFDNSNINLSLPIAGMIVYQNGKLNNKLNRKYNLMTTKNASDYHFMIEVITRRYQRLVSQHQKLPNLIVVDGGKLQVNAALYALAQLQINIPLIGLKKDQKHKTDAIVLTNGDEIVLDRKSILYKFLANMQNDVHNYAISFLRDKHTKSIFNSLLDNVQGLGKKRFNELLKYYDSINDLKSASDQELLQFLPKNVLVNLREKLNKI", "aa_hexdigest": "abc80b6fe4be013572bb0e083bc7ff77", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_Q9PQA9", "query_cov": 1.0, "subject_cov": 0.8253424657534246, "identity": 1.0, "score": 946.0, "evalue": 0.0, "valid": true, "gene": "uvrC", "product": "excinuclease ABC subunit UvrC", "uniref50_id": "UniRef50_Q9PQA9", "cog_id": "COG0322", "cog_category": "L", "go_ids": [ "GO:0003677", "GO:0005737", "GO:0006289", "GO:0009380", "GO:0009381", "GO:0009432" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQA9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQA9" ], "product": "UvrABC system protein C" }, "genes": [ "uvrC" ], "id": "NKIECMJPJD_830", "locus": "GCA000828735_00817" }, { "type": "cds", "sequence": "contig_1", "start": 486303, "stop": 486500, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAACATTAGTTTTATACGCACGCGTTTGTCCAACTAAATCGTTTCATACACAACAAATTTTTGAAGCTTTTCAAAAACAAACAAATTTTGTTTGTGTTAATCTTGATGCAAATCAACCTTTTGATGTTTCAAAAGAACAAGAATTTATTTTAGGGTTTGATCGAATTATTTTATTGTTTACGCTAAATTGA", "aa": "MKKTLVLYARVCPTKSFHTQQIFEAFQKQTNFVCVNLDANQPFDVSKEQEFILGFDRIILLFTLN", "aa_hexdigest": "5539e9c4a2641ce28a59d447372f8258", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7628.800799999997, "isoelectric_point": 7.795726585388184 }, "id": "NKIECMJPJD_831", "locus": "GCA000828735_00818" }, { "type": "cds", "sequence": "contig_1", "start": 486666, "stop": 486809, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATAATGTAAATGGAATGCTGAAAAAATTACGAGCAAGTATTCAAAAAAGTTTTTATTATTATGGTGCAATTAATCAAGATCAAATGCGTTTAAATCAATTTGTTAATGAATTAATAAAATATTTTAAAAATCAAAATTAA", "aa": "MNNVNGMLKKLRASIQKSFYYYGAINQDQMRLNQFVNELIKYFKNQN", "aa_hexdigest": "bd4753f8c72a90616a269d751e84a41c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5677.4968, "isoelectric_point": 9.90023250579834 }, "id": "NKIECMJPJD_832", "locus": "GCA000828735_00819" }, { "type": "cds", "sequence": "contig_1", "start": 487097, "stop": 487372, "strand": "+", "frame": 2, "gene": null, "product": "Conserved hypothetical", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQB2", "UniRef:UniRef90_Q9PQB2" ], "nt": "ATGGCTAACGTGATTCATAATATCAAAGAATTACAAACGTTAACCATTAACAAAAAAATTAAAATTAAAGATTTTGATTACTTATCTTATTTACCTTTGGTAAAAGATGATCCATTAGCTTCATATTTTAAATCTATTTATGAACAAGAAGTAAAAAGAACTAAACTTGTTTTGGCTCACAATGATTTTTCAATTAATAACATTTTAGCAAATGAATGCGAAAATCTTTTTATATTAATTGATTTTGAATATAGTAGTAGAAATTTAAAAGATTGA", "aa": "MANVIHNIKELQTLTINKKIKIKDFDYLSYLPLVKDDPLASYFKSIYEQEVKRTKLVLAHNDFSINNILANECENLFILIDFEYSSRNLKD", "aa_hexdigest": "b709704140ecf10c0490a1f21c0d87b2", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 487067, "stop": 487612, "observations": { "mutation": [ 487091, 487370, 487508 ], "stop": [ 487091, 487370, 487508 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQB2", "query_cov": 1.0, "subject_cov": 0.4972677595628415, "identity": 1.0, "score": 182.0, "evalue": 3.95e-57, "gene_start": 1, "gene_end": 91, "reference_start": 13, "reference_end": 103, "reference_sequence": "MSKNPYVINEWFMANVIHNIKELQTLTINKKIKIKDFDYLSYLPLVKDDPLASYFKSIYEQEVKRTKLVLAHNDFSINNILANECENLFILIDFEYSSRNLKDWDLFNFLRDLNDVQLNFGISFIKEKLGYDECLIYKYLFLTSYYGFLWATNAFLKTNELKIKIYGENAYQKADFYLQKLNK" }, "paralog": false, "identity": 0.9779005524861878, "score": 353.0, "evalue": 6.95e-127, "description": "Internal stop codon at 487091, 487370, 487508. Nonsense mutation around 487091, 487370, 487508.", "psc": { "uniref90_id": "UniRef90_Q9PQB2", "product": "Conserved hypothetical", "uniref50_id": "UniRef50_Q9PQB2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQB2", "UniRef:UniRef50_Q9PQB2" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQB2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQB2" ], "product": "Conserved hypothetical" } }, "id": "NKIECMJPJD_833", "locus": "GCA000828735_00820" }, { "type": "cds", "sequence": "contig_1", "start": 487725, "stop": 487904, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTCTAGCTGGAATTCATGGGGAAGCTGCAACAAAAATGTTTGGTGCATTAATGAAAGAACATAAAGAAATTGCCAATTTTGCAATTACTAACCGCCGATTTAATGATGAAACAAGAAAAGTAGTTGCTCCTCAAGATCGCGATAAATACTCTCAAAATGCAGCTCTTGTTGATTGA", "aa": "MILAGIHGEAATKMFGALMKEHKEIANFAITNRRFNDETRKVVAPQDRDKYSQNAALVD", "aa_hexdigest": "11a7d099d408d39e880d15345292e4f9", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6633.510600000003, "isoelectric_point": 8.141212654113772 }, "id": "NKIECMJPJD_834", "locus": "GCA000828735_00821" }, { "type": "cds", "sequence": "contig_1", "start": 488139, "stop": 488309, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCAAAATCTGAATTAGATGCTTTAAAAGCTAAAGAAGAATTTAAAGTGCCAAAACAATTAGTTACATTAGTTAACCAAGCTACAGATTTAAAAGAAGATAAACAAACAATTAAAGATGAATGCGATCCTTTTAAATCACACCGTGATCCAAATCATAAAATTGACTGA", "aa": "MPKSELDALKAKEEFKVPKQLVTLVNQATDLKEDKQTIKDECDPFKSHRDPNHKID", "aa_hexdigest": "99e94d505b94ff4f1ff39ba4d34567a5", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6491.320700000002, "isoelectric_point": 6.071052360534667 }, "id": "NKIECMJPJD_835", "locus": "GCA000828735_00822" }, { "type": "cds", "sequence": "contig_1", "start": 488373, "stop": 488471, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGAGTCAAAATGCTAATGATTTCGTTGCAAAACGCATGTTAAAAAATCCAAATAATAAGGACGATTTAATTGTTATGCCAATGAGTAGTTTATGA", "aa": "MKSQNANDFVAKRMLKNPNNKDDLIVMPMSSL", "aa_hexdigest": "29695fa9393432f717efd3689f2669b8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3651.2629000000006, "isoelectric_point": 9.522705650329591 }, "id": "NKIECMJPJD_836", "locus": "GCA000828735_00823" }, { "type": "cds", "sequence": "contig_1", "start": 488472, "stop": 488801, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGTAACATCAATTGATTTACCTGCTGATTTTCCAATTATTTATTCAAAACCTGATTATGATGGTATTCCTGGATTAGGTGCACGTTTTCCAACACCTAAATCAAAAGTAGCTCCTGAAAAATTATTAGATGATGGTTTCTCATTTGGAGATATTATTGTTGAAGATGGTTCAGATACTCAAAAAGCAAATCTACCTGGTCAATTAATTGAATCATTTGAAAAGACAGCTGATAAAGTAATTTACTTATACTATGATTCAGGTTTACCAGAAGCGTTTAAAAACAATCAACGTCAACCTGAAAAAATTAAACAATTTGAAGACTGA", "aa": "MNVTSIDLPADFPIIYSKPDYDGIPGLGARFPTPKSKVAPEKLLDDGFSFGDIIVEDGSDTQKANLPGQLIESFEKTADKVIYLYYDSGLPEAFKNNQRQPEKIKQFED", "aa_hexdigest": "5c1ad5b4f5a24e83ccdf18432d25a4dc", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12159.481199999991, "isoelectric_point": 4.49666919708252 }, "id": "NKIECMJPJD_837", "locus": "GCA000828735_00824" }, { "type": "cds", "sequence": "contig_1", "start": 488877, "stop": 489026, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATAAAGCAGTTGATTTAACAAAATCTTCAAATTTTAAAAAATTAATTGCTGATGGAAAATACAAATCTACATTTAGTGCACAAATTGAAGATATTATTACTAAAGTTAAACATGATATTAAAATGTATGCAGAATCACCATTTTGA", "aa": "MDKAVDLTKSSNFKKLIADGKYKSTFSAQIEDIITKVKHDIKMYAESPF", "aa_hexdigest": "617f39365e40223e8e342c26f6fa5421", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5582.403399999999, "isoelectric_point": 9.005153465270997 }, "id": "NKIECMJPJD_838", "locus": "GCA000828735_00825" }, { "type": "cds", "sequence": "contig_1", "start": 489078, "stop": 489332, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAATAAATAAAAAGATTTTGTTTTCTTCATTGTTATTTGGAACTATAGCTATTGGAACTGTTGCAGTTGCAACTGCATGTAGTGACAATAAGAAAACTAAAAAAACAATCAACACAGGCACTACTCAACCCGGAAGTAGTACAGGAACAACAAGTAAATCATTAACGCAAGAAAAAGTGTTAAGAAATGAAATCATTTCTGAAATTTTGAATGCTAAATCTAAAAAACCAGATCGTGATAAGATGTGA", "aa": "MKKINKKILFSSLLFGTIAIGTVAVATACSDNKKTKKTINTGTTQPGSSTGTTSKSLTQEKVLRNEIISEILNAKSKKPDRDKM", "aa_hexdigest": "47df60c03fce163be1d220702cad54bf", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9057.475299999995, "isoelectric_point": 10.130384635925292 }, "id": "NKIECMJPJD_839", "locus": "GCA000828735_00826" }, { "type": "cds", "sequence": "contig_1", "start": 489447, "stop": 489743, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF14579.12" ], "nt": "GTGATTGAATTATTAGCTCGAGGTTTTAAAATTTCAAATATTAGTATTGAACAATCACAGGCGACTAGTTATGTAATTGATAAAGAAAACAATGCTATTATTCCACCATTTATTGCGATTCAAGGATTGGGTGAAACAGTAGCTAATTCAATCATTGAAGCTCGTAATCAAAAAGTTTTTTCAACAATTGAGGATTTAAAAAACCGTACAAAAATTTCGCGTACAGATTTAAAAAATTTACGAGTATTAGGCGTTTTAGATCATTTAAGTGAAACTGAACAACTAACATTATTTTAA", "aa": "MIELLARGFKISNISIEQSQATSYVIDKENNAIIPPFIAIQGLGETVANSIIEARNQKVFSTIEDLKNRTKISRTDLKNLRVLGVLDHLSETEQLTLF", "aa_hexdigest": "d552a72f953817a53c4397f038bb1022", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF14579.12", "name": "HHH_6", "length": 89, "aa_cov": 0.8673469387755102, "hmm_cov": 0.9662921348314607, "evalue": 1.517413420417995e-16, "score": 55.97451400756836, "start": 8, "stop": 92 } ], "seq_stats": { "molecular_weight": 10985.493199999986, "isoelectric_point": 5.7753190994262695 }, "id": "NKIECMJPJD_840", "locus": "GCA000828735_00827" }, { "type": "cds", "sequence": "contig_1", "start": 490029, "stop": 490193, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF17657.6" ], "nt": "TTGCTAAAGGATGTTATTGCTTGTCGTGATGATATTATGACATATTTAATTCGTCAAAATGTTGAACCAAAAACTGCCTTTTTAATTATGGAAGATGTTAGAAAAGGTAAAAAGATCAAACCAGAACACCAAATCATTTTAAAAGAATTAAAGGTTCCTGAATGA", "aa": "MLKDVIACRDDIMTYLIRQNVEPKTAFLIMEDVRKGKKIKPEHQIILKELKVPE", "aa_hexdigest": "078d8c3ba034593f920e08e45a29ee70", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF17657.6", "name": "DNA_pol3_finger", "length": 54, "aa_cov": 0.7962962962962963, "hmm_cov": 0.9814814814814815, "evalue": 8.284354734682347e-07, "score": 24.0496768951416, "start": 2, "stop": 44 } ], "seq_stats": { "molecular_weight": 6379.6242999999995, "isoelectric_point": 8.852105522155764 }, "id": "NKIECMJPJD_841", "locus": "GCA000828735_00828" }, { "type": "cds", "sequence": "contig_1", "start": 490230, "stop": 490436, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF17657.6" ], "nt": "ATGTATGATCCATTAGTTATGAAATCATTTAGCGATATTAGTGCGCTAAATATTAAACCGTCTGATGTTTTAAATGAAACAACAGGAGCAATTTCTATTCCTGAATTTGGGACACGCTTTGTACGTGGTATGCTAGTGGATACTAAACCAAAATCATTTGCTGACTTAATTCGTATTTCAGGATTATCACATGGAGAAAGTGTTTGA", "aa": "MYDPLVMKSFSDISALNIKPSDVLNETTGAISIPEFGTRFVRGMLVDTKPKSFADLIRISGLSHGESV", "aa_hexdigest": "fc2ba38691c2d0004987e5fb1118893d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF17657.6", "name": "DNA_pol3_finger", "length": 65, "aa_cov": 0.9558823529411765, "hmm_cov": 0.7692307692307693, "evalue": 4.908438298466176e-12, "score": 41.05399703979492, "start": 3, "stop": 67 } ], "seq_stats": { "molecular_weight": 7401.45, "isoelectric_point": 5.608780860900879 }, "id": "NKIECMJPJD_842", "locus": "GCA000828735_00829" }, { "type": "cds", "sequence": "contig_1", "start": 490566, "stop": 491159, "strand": "-", "frame": 1, "gene": null, "product": "DNA polymerase III polC-type", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A180EXR3" ], "nt": "ATGCAAGATGGTTATGGTGTTGGTAGTCGTGGTTCGGTTGGCTCATCACTTGTCGCAACGTTTTTAAATATTACTGACGTTAATCCGCTTACACCACATTATTTATGTCCTAATTGTAAAAAATGTGAATTTATAACAAACGCTGATGATGGATTTGATTTAGCTCCTAAAAGTTGTGAGCAGTGTCAAACTCCAATGTTAACTGATGGACATAATATTCCTTTTGAAACTTTTTTAGGTTTTGATGGGGATAAAGTACCAGATATTGATCTTAATTTTTCTGGAGTTTATCAAGCAGTTGCACATAATTTTATTAAAAGTATTTTTGGAGAAACACATTCTTATCGTGCTGGTACAATTGGTACTATGGCCCAAACAAGCGCTGAAAATACGGTTAAGAAGTATTTTGAAAACCGTTTTAATGAAAATAAAATTATTCGTGATTCAACGGTTAGTTTATATGTACAAAAGTGCATTGATTCTAAACGCACAACTGGTCAACATCCTGGGGGTATTATTATTGTTCCTAAAGAATATAGTATTTGGGATTTTTCACCTTATAATTTTCCAGCTAATGATATTAATGAAACTTGA", "aa": "MQDGYGVGSRGSVGSSLVATFLNITDVNPLTPHYLCPNCKKCEFITNADDGFDLAPKSCEQCQTPMLTDGHNIPFETFLGFDGDKVPDIDLNFSGVYQAVAHNFIKSIFGETHSYRAGTIGTMAQTSAENTVKKYFENRFNENKIIRDSTVSLYVQKCIDSKRTTGQHPGGIIIVPKEYSIWDFSPYNFPANDINET", "aa_hexdigest": "13ba336ba48f4c53f2d3fe8615352afd", "start_type": "ATG", "rbs_motif": "AGxAG", "psc": { "uniref90_id": "UniRef90_A0A180EXR3", "query_cov": 0.9847715736040609, "subject_cov": 0.8326180257510729, "identity": 0.619, "score": 248.0, "evalue": 1.38e-80, "valid": false, "product": "DNA polymerase III polC-type", "uniref50_id": "UniRef50_A0A180EXR3" }, "pscc": { "uniref50_id": "UniRef50_A0A180EXR3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A180EXR3" ], "product": "DNA polymerase III polC-type" }, "genes": [], "id": "NKIECMJPJD_843", "locus": "GCA000828735_00830" }, { "type": "cds", "sequence": "contig_1", "start": 491187, "stop": 491333, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF07733.18" ], "nt": "ATGGAAGGTGTTGATCAAAAAACAATCGATTATGTTTATCATACTGCTAAAAAAATATATGGAGAGAATTTACCAACAATTGTTGAACAGCGCATTAAAAAAGAATTAAATTCAATTATTAAACATGGTTTTAGTGTTGTTTATTGA", "aa": "MEGVDQKTIDYVYHTAKKIYGENLPTIVEQRIKKELNSIIKHGFSVVY", "aa_hexdigest": "3a1dab87c775e6a09ded7d963af2442e", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07733.18", "name": "DNA_pol3_alpha", "length": 40, "aa_cov": 0.8333333333333334, "hmm_cov": 1.0, "evalue": 2.2086417477977385e-07, "score": 26.284317016601562, "start": 9, "stop": 48 } ], "seq_stats": { "molecular_weight": 5597.3994999999995, "isoelectric_point": 8.026136589050292 }, "id": "NKIECMJPJD_844", "locus": "GCA000828735_00831" }, { "type": "cds", "sequence": "contig_1", "start": 491382, "stop": 491924, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATGAATTAGTGTCTATATCTCATACTGATATGTATTATACAAGACCAACAATTACGACAAGCTTTTTAGCAAATAAAAAAGATAAATTAATTATTTCTAACTCGATTCACGAATCAGATTTAATCAATGCTTTATATTCAAAAAACGATGAAGAAATTAAAAGGTTAATTCAACGTTATGATTTTATAACATTACCATCATTAGGTTCACAAAAACACTTAGTATATGCTAAAAAAATTACAATTGAAAATGTACAAAAAGCTTTTAAAAAATTGATCTATTTAGCTTTAGAATTAAACAAGATTATTATTTATTCAAGCTCGCCTTATTATTTCTTTAAAGATGATAAAAAATTTTATGATGTTTATGTTAATACGAAAGGTCTCGAAGGTAAAGCACACCGATTTGCTAATGAAGTTTATGTTCCTGATTTAGAATATATTGATCAAAAAAATGCCATTGATGAATTAGCTTATTTAGAAGATGAAAAGTTAATTAACTTGATTATTAATGAGAATCCTGTGCATATTAATAGTTGA", "aa": "MYELVSISHTDMYYTRPTITTSFLANKKDKLIISNSIHESDLINALYSKNDEEIKRLIQRYDFITLPSLGSQKHLVYAKKITIENVQKAFKKLIYLALELNKIIIYSSSPYYFFKDDKKFYDVYVNTKGLEGKAHRFANEVYVPDLEYIDQKNAIDELAYLEDEKLINLIINENPVHINS", "aa_hexdigest": "547ec0a38ed7205273fb3ff6557502fc", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 21115.935400000002, "isoelectric_point": 6.214002418518065 }, "id": "NKIECMJPJD_845", "locus": "GCA000828735_00832" }, { "type": "cds", "sequence": "contig_1", "start": 491964, "stop": 492203, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATATCACGAGCAATAAATCCATTTAAAAGTCATCGATTAGGAGCGATTTGTAAAAAATATGAAGTTGATTATAATGATGAAAGTGCACACCGTGCAGACTATGATGCCATAGTTTTAGCTGATGTTTTTAAAGTTATGAAAAATAACTTATTTAATGATTTTGGAATTACTAATTTAAGTGAAATTAATACTAAATTACAAACAACAATGTTAAAAAATCGTAGTTTTGGTAATTGA", "aa": "MISRAINPFKSHRLGAICKKYEVDYNDESAHRADYDAIVLADVFKVMKNNLFNDFGITNLSEINTKLQTTMLKNRSFGN", "aa_hexdigest": "18814a64b70ab0afbfadc870a9b72c61", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9058.235099999994, "isoelectric_point": 8.818839836120606 }, "id": "NKIECMJPJD_846", "locus": "GCA000828735_00833" }, { "type": "cds", "sequence": "contig_1", "start": 492243, "stop": 493115, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13482.12", "PFAM:PF00929.31" ], "nt": "ATGAATAATGCAAAAATTCGAGCTTATGTTTTTTTAAAAAAGATTGATATATTAAAAAGTGGAGCAATAGCTTATAAACTAAATGTTATTGATGATAGTGAAACCTTAACAATTATGACTTATTTACCAAGCGGTGAACATCCTTTAAAAAAGTTTTTAGATGAACTGAAAATTGATCAATTAATCGAGGCTGAAATTGATATTGTTTTAGATAATATGAGTAAGAGTGGTCAAGTTCCTATTGGTAAAATTAAAAAAATTTGTTGCGTTGAAGATAAACATGTAAAAAAACAAATCACACCTCGTTTAGAACTAAATTTTCATACAAAAATGTCATCACTTGATGCAATTATTTCGACACAAGAATTAATTGATTTTGCGGTTAAGAATCAATTAAAAACAATTGGCATCACTGATCGAAATGTTGTACAAGCATATCCTGAAATCGCTAAATTTTCTAAAAAACAAGATTTAAAAATTATTTATGGTTTAGAAACAGAAGAATTAGAAGATCAAATCCCATTAGTTTTAAATGTTCGGGATCAAAATTTAGATAATGCTACATATGTTATTTTTGATATTGAAACAACAGGATTATTTCCTAATTTTGATGAAATTATTGAATTTGGGGCCGTGATTATGCAAAATAATAAGCAAATTGGGGAAAAAATTCAATTTTTTATAAAACCTATTCAACAAATTAATGAGAATGTTACTAATCTAACTAATATTAGTCAAGAAATGGTCAATAATGCAATTGATGAAAAAACAGCATTATTAAAAATTAAAGAAATTTTTGATGACCATATTTTAGTTGCACATAATGGAATTAATTTTGATATTAATTTTATTAATCAGAGACTGTTAAAATGA", "aa": "MNNAKIRAYVFLKKIDILKSGAIAYKLNVIDDSETLTIMTYLPSGEHPLKKFLDELKIDQLIEAEIDIVLDNMSKSGQVPIGKIKKICCVEDKHVKKQITPRLELNFHTKMSSLDAIISTQELIDFAVKNQLKTIGITDRNVVQAYPEIAKFSKKQDLKIIYGLETEELEDQIPLVLNVRDQNLDNATYVIFDIETTGLFPNFDEIIEFGAVIMQNNKQIGEKIQFFIKPIQQINENVTNLTNISQEMVNNAIDEKTALLKIKEIFDDHILVAHNGINFDINFINQRLLK", "aa_hexdigest": "66e15c1c44162f688a8f715a381c6a2a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13482.12", "name": "RNase_H_2", "length": 99, "aa_cov": 0.3413793103448276, "hmm_cov": 0.7878787878787878, "evalue": 1.2439270980430532e-07, "score": 27.336830139160156, "start": 190, "stop": 288 }, { "id": "PF00929.31", "name": "RNase_T", "length": 104, "aa_cov": 0.3413793103448276, "hmm_cov": 0.9903846153846154, "evalue": 2.3575244078932834e-25, "score": 85.56636810302734, "start": 190, "stop": 288 } ], "seq_stats": { "molecular_weight": 33161.05470000001, "isoelectric_point": 5.284230232238768 }, "id": "NKIECMJPJD_847", "locus": "GCA000828735_00834" }, { "type": "cds", "sequence": "contig_1", "start": 493578, "stop": 493775, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAACAAAGAATGCATTATTTAAAAAGATTGTAAAAATTTCTGATCAATTATTAAATAAAATTAGTATCAAAAAATTAACTAAAGATAAAAAAAATAATTTATTTGTTTATTTTGACGAATTTGTTGATGTAACAATTATTGATGAATTACATCAATTATCAAAAACAACATTAATGCATGATTTACAAATTTGA", "aa": "METKNALFKKIVKISDQLLNKISIKKLTKDKKNNLFVYFDEFVDVTIIDELHQLSKTTLMHDLQI", "aa_hexdigest": "424a6f1cb1ad27ce5e6be2c81619bea2", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7679.9888999999985, "isoelectric_point": 8.964731788635255 }, "id": "NKIECMJPJD_848", "locus": "GCA000828735_00835" }, { "type": "cds", "sequence": "contig_1", "start": 493924, "stop": 494157, "strand": "+", "frame": 1, "gene": null, "product": "Secreted protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI0005A51F2F", "UniRef:UniRef100_A0AAC9T3N0", "UniRef:UniRef50_A0A2C9DY03", "UniRef:UniRef90_A0A2C9DY03" ], "nt": "TTGATATTTTATATTTTTAATTTGAATTATCAAACAGAAAAAGAATTAACAACTGCTAAAACTGATGTTGAAAGCAAACAAAAAGTTGCTGATGCTGCTAAAACAAAATTAGATGAAGCAACTAAAGAATCAAAAAACGCAAATCAATTATTAGAAACAGCTAAAACTAATCAAACAACAAAACAAGAAGCAGTTGATAAAGCTAAAGAAGAATTAGAAGAAGCTAAAAAATAA", "aa": "MIFYIFNLNYQTEKELTTAKTDVESKQKVADAAKTKLDEATKESKNANQLLETAKTNQTTKQEAVDKAKEELEEAKK", "aa_hexdigest": "6834c384421d571a43e7b3bc1503ec19", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0005A51F2F", "uniref100_id": "UniRef100_A0AAC9T3N0", "db_xrefs": [ "SO:0001217", "UniParc:UPI0005A51F2F", "UniRef:UniRef100_A0AAC9T3N0" ] }, "ips": { "uniref100_id": "UniRef100_A0AAC9T3N0", "uniref90_id": "UniRef90_A0A2C9DY03" }, "psc": { "uniref90_id": "UniRef90_A0A2C9DY03", "product": "Secreted protein", "uniref50_id": "UniRef50_A0A2C9DY03" }, "pscc": { "uniref50_id": "UniRef50_A0A2C9DY03", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2C9DY03" ], "product": "Secreted protein" }, "genes": [], "id": "NKIECMJPJD_849", "locus": "GCA000828735_00836" }, { "type": "cds", "sequence": "contig_1", "start": 494299, "stop": 495048, "strand": "-", "frame": 3, "gene": null, "product": "putative protein UU376", "db_xrefs": [ "RefSeq:WP_012316982.1", "SO:0001217", "UniParc:UPI000165F8D0", "UniRef:UniRef100_A0A2C9DXY4", "UniRef:UniRef50_Q9PQB5", "UniRef:UniRef90_Q9PQB5" ], "nt": "TTGAATTATCAAACAGAAAAAGTGAACTTTGAAGCAGCTTCAGCTCCAAGCCAACAACCATCTCCGACTCCAGCTCCAAAAACTGAAGAACCAAAAGAAAATGGTGGTAGCGAACAGCAAGCTGATCAACCAGAAAATTCTAAAAAAGATGATGTTGTAGCTAGTGGTGTAAAAATTAGTGAAGTCAAAAATAATACTGCTACTATTGAAGTTACATTTAGTAAATTTGAATTAGCAGATGCTAATAAAAAAGATTTTGTTTTAGAAGTTATTAAGAAAGCTGATACAAATCCAATTCAAGCAAGTGATTTAAAATATGATGAAGCATCTAAAACATTAAGCGGTAAACTAAGTGGTTTAAATTCAAATGTTGACTATGAAATTTCAAAACTAACTTTAAATGGTAAAGAAGTCAAATTTAATGAAGAAGAGTTGTTAAAATCATATGTTAAAAATGCAAAATTATTTATGACATTCAACAAAGAAAATAAGAAAATTAATGTTAAATTACAAAATTTTGATATTTTAAATTCTTTAGAAGAAAATCAACCTATATTAACATTTGATATTGAAATTAAAAAAGATAGTAGTTTACAAGTAGTTCATAAATCATTAACAAAGAATCAATTATCAAATCTTAATGGTTTAGAAATTGATTTAAAAGATAAAATGAATGGAAATAATGCAAATTATGATGTTAAATTAACCAATGCTAAATTGTTAAATGTTAACATAGAAACACAAAAATAA", "aa": "MNYQTEKVNFEAASAPSQQPSPTPAPKTEEPKENGGSEQQADQPENSKKDDVVASGVKISEVKNNTATIEVTFSKFELADANKKDFVLEVIKKADTNPIQASDLKYDEASKTLSGKLSGLNSNVDYEISKLTLNGKEVKFNEEELLKSYVKNAKLFMTFNKENKKINVKLQNFDILNSLEENQPILTFDIEIKKDSSLQVVHKSLTKNQLSNLNGLEIDLKDKMNGNNANYDVKLTNAKLLNVNIETQK", "aa_hexdigest": "8cf01ecd9319052bc39a6c69c35a265e", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000165F8D0", "ncbi_nrp_id": "WP_012316982.1", "uniref100_id": "UniRef100_A0A2C9DXY4", "db_xrefs": [ "SO:0001217", "UniParc:UPI000165F8D0", "RefSeq:WP_012316982.1", "UniRef:UniRef100_A0A2C9DXY4" ] }, "ips": { "uniref100_id": "UniRef100_A0A2C9DXY4", "uniref90_id": "UniRef90_Q9PQB5" }, "psc": { "uniref90_id": "UniRef90_Q9PQB5", "product": "Uncharacterized protein UU376", "uniref50_id": "UniRef50_Q9PQB5" }, "pscc": { "uniref50_id": "UniRef50_Q9PQB5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQB5" ], "product": "Uncharacterized protein UU376" }, "genes": [], "id": "NKIECMJPJD_850", "locus": "GCA000828735_00837" }, { "type": "cds", "sequence": "contig_1", "start": 495392, "stop": 496228, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACATTAGGTGTTACCTTAGTTGGAGCTGGAATAGTTGCTATAGCAGCTTCATGTTCTAATTCAACTGTTAAATCTAAGTTAAGTAACCAATTTGCTAAATCAACAGACGGTAAAAGTTTTTATGCGGTTTACGAAATTGAAAACTTTAAAGATCTAAGTAATGATGATAAAAAATCATTAAGTAACATTGAATTTAATGCTGCACTTACATCAGCTGAAAACAAAACAGAAAGTACACTTGAAAAAGGTCATTTAGTTGGTGAAAAAATTTACGTTAAATTACCTCGTGAACCAAAACCTAATGAACAATTAACTATTATTAGTAAAAGTGGATTAATCAAGACTTCAGGTTTGTTAATATCTGATAATTTGAATTATCAAACAGAAAAAGTGAACTTTGAAACTACACAACCAGGTAAAGAACAACCAGCAGGTAAAGAACAACCAGCAGGTAAAGAACAACCAGCAGGTAAAGAACAACCAGCAGGTAAAGAACAACCAGCAGGTAAAGAACAACCAGCAGGTAAAGAACAACCAGCAGGTAAAGAACAACCAGCAGGTAAAGAACAACCAGCAGGTAAAGAACAACCAGCAGGTAAAGAACAACCAGCAGGTAAAGAACAACCAGCAGGTAAAGAACAACCAGCAGGTAAAGAACAACCAGCAGGTAAAGAACAACCAGCAGGTAAAGAACAACCAGCAGGTAAAGAACAACCAGCAGGTAAAGAACAACCAGCAGGTAAAGAACAACCAGCAGGTAAAGAACAACCAGCAGGTAAAGAACAACCAGCAGGTAAAGAACAACCAGCAGGTAAAGAAACTACTGGAAAATAA", "aa": "MTLGVTLVGAGIVAIAASCSNSTVKSKLSNQFAKSTDGKSFYAVYEIENFKDLSNDDKKSLSNIEFNAALTSAENKTESTLEKGHLVGEKIYVKLPREPKPNEQLTIISKSGLIKTSGLLISDNLNYQTEKVNFETTQPGKEQPAGKEQPAGKEQPAGKEQPAGKEQPAGKEQPAGKEQPAGKEQPAGKEQPAGKEQPAGKEQPAGKEQPAGKEQPAGKEQPAGKEQPAGKEQPAGKEQPAGKEQPAGKEQPAGKEQPAGKEQPAGKEQPAGKETTGK", "aa_hexdigest": "6c3a49f184a997155a9f95b34d2349b4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 29243.177399999917, "isoelectric_point": 7.642423057556152 }, "id": "NKIECMJPJD_851", "locus": "GCA000828735_00838" }, { "type": "cds", "sequence": "contig_1", "start": 496726, "stop": 496830, "strand": "+", "frame": 1, "gene": null, "product": "Unique hypothetical", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1C41", "UniRef:UniRef100_Q9PQB8", "UniRef:UniRef50_Q9PQB8", "UniRef:UniRef90_Q9PQB8" ], "nt": "ATGTCAAGAGAACAACTTGAATATCTCATTACTGATATTTTTTATGAAGAAATTTTAAAACGCAACAATAGCAAAAATTTAGAAGAAGTTGTTGAAAAAAATTAA", "aa": "MSREQLEYLITDIFYEEILKRNNSKNLEEVVEKN", "aa_hexdigest": "09e3cdbab7465aa002f7715dd52c6690", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00000C1C41", "uniref100_id": "UniRef100_Q9PQB8", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1C41", "UniRef:UniRef100_Q9PQB8" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQB8", "uniref90_id": "UniRef90_Q9PQB8" }, "psc": { "uniref90_id": "UniRef90_Q9PQB8", "product": "Unique hypothetical", "uniref50_id": "UniRef50_Q9PQB8" }, "pscc": { "uniref50_id": "UniRef50_Q9PQB8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQB8" ], "product": "Unique hypothetical" }, "genes": [], "id": "NKIECMJPJD_852", "locus": "GCA000828735_00839" }, { "type": "cds", "sequence": "contig_1", "start": 496899, "stop": 497666, "strand": "+", "frame": 3, "gene": null, "product": "DDE-type integrase/transposase/recombinase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XED4", "UniRef:UniRef90_A0A6A8PZ83" ], "nt": "TTGCCAGCCAAAAACAAGGCAATTCAATATGAAAAAGAAATAAAAGAGGCTTTTATTGAATGCAAAGGAAGATTTGGAAGGGAAAGACTTTCACTTTTTCTTAAGAAAAAGTACCAAATCGATATAAACCCAAGGACTCTGGGCAGAATTATGAATAAATTGAGTTTGCTATGCAAAATAAGAAGAAGCAAAAGAAAAAGAGAAATAAAGAATGTTAATGCAACATTCAAAAACTTAGCCAATAGAGATTATGATGGCCAAATGAATGATATATATGCTACCGATGTGACATATATTCCTTCACCCACTGATGTTATAGAAAATCATGTATATTTATCGGTTTTATTGCATCATAAAACAAAGAAAATTGTGAGCTGGAATTTATCAAAATATAATGATACTAATCTTGTTTTAAAACATTTTAAACTAACTAAATTTCCTAAAAAATTCATTGTACATTCAGATCACGGATATCAATATTCCTCTAAGGAATTCCTTGATTACATAGATTCAATTAATGGCACTATTTCTATGTCAAGAGTTGGAAATTCTTTGGATAATAGAGAAGTAGAATATTTCTTTTCTATTCTAAAAACTGAAATATTTTCAAATTTTTTTCAAAAAGCAAAAAGTTTAACATTTAATGAATTGCAAATAGAAATAGACAAATTTATTAATTGGTATAATAATGAAAGATTTATCAAAAAATTTGATTTAAAAACACCCCAACAAATGTGGGATGTTTATATGAACAATAAATCTATTTGA", "aa": "MPAKNKAIQYEKEIKEAFIECKGRFGRERLSLFLKKKYQIDINPRTLGRIMNKLSLLCKIRRSKRKREIKNVNATFKNLANRDYDGQMNDIYATDVTYIPSPTDVIENHVYLSVLLHHKTKKIVSWNLSKYNDTNLVLKHFKLTKFPKKFIVHSDHGYQYSSKEFLDYIDSINGTISMSRVGNSLDNREVEYFFSILKTEIFSNFFQKAKSLTFNELQIEIDKFINWYNNERFIKKFDLKTPQQMWDVYMNNKSI", "aa_hexdigest": "7497545546a9c87e72490047962a3681", "start_type": "TTG", "rbs_motif": "4Base/6BMM", "psc": { "uniref90_id": "UniRef90_A0A6A8PZ83", "query_cov": 1.0, "subject_cov": 0.9922178988326849, "identity": 0.976, "score": 499.0, "evalue": 2.41e-178, "valid": true, "product": "DDE-type integrase/transposase/recombinase", "uniref50_id": "UniRef50_C4XED4" }, "pscc": { "uniref50_id": "UniRef50_C4XED4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XED4" ], "product": "Integrase catalytic domain-containing protein" }, "genes": [], "id": "NKIECMJPJD_853", "locus": "GCA000828735_00840" }, { "type": "cds", "sequence": "contig_1", "start": 497866, "stop": 498237, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08264.19" ], "nt": "ATGATGGTTTTTGTTAATGTTTTATACAAAAATAAAGTTATTAATTATGAATTATTAGATAATTTTTTAATTTTACTTTCATGCTATGCACCACATTTAGCGGAGGAATTATATAGTTTAAACCATTCAGAATCTGTTTGTTTACAAAAAATGCCTATTTATGATGAACAAAAAATTATTGCTCAAAATATTACAATTCCAATTCAAATTAATGGTAAATTAAAGCACACAATCAATGTTTTAAGAGATACAAATGCTGAAGAGTTAGTTAATTTAGCATTGGCTTGTGAACAAGTTAAACAAGCAATTGGGGATCAACCAATTAAAAAACAAATCGTTGTTGTTAATAAAATTATTAATTTTGTTATTTAA", "aa": "MMVFVNVLYKNKVINYELLDNFLILLSCYAPHLAEELYSLNHSESVCLQKMPIYDEQKIIAQNITIPIQINGKLKHTINVLRDTNAEELVNLALACEQVKQAIGDQPIKKQIVVVNKIINFVI", "aa_hexdigest": "2d60350544ec806e064b1c918717391a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08264.19", "name": "Anticodon_1", "length": 83, "aa_cov": 0.5934959349593496, "hmm_cov": 0.9879518072289156, "evalue": 1.547387745282043e-13, "score": 46.39335250854492, "start": 15, "stop": 87 } ], "seq_stats": { "molecular_weight": 14064.45959999998, "isoelectric_point": 6.268567848205566 }, "id": "NKIECMJPJD_854", "locus": "GCA000828735_00841" }, { "type": "cds", "sequence": "contig_1", "start": 498430, "stop": 498633, "strand": "-", "frame": 3, "gene": null, "product": "class I tRNA ligase family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A359BCU9", "UniRef:UniRef90_UPI0013027DF7" ], "nt": "TTGTATGATATTGGTGTAGTGCCAACAAAAGAACCATTTTATAAAGTAATTAATCAGGGAATGATTTTAGGCGAAAATAATGAAAAAATGTCTAAATCAAAAGGGAATGTTATTAATCCTGATGATATTATTGCTTCGCATGGTGCTGATACATTAAGAATTTATGAAATGTTTATGGGACCATTAACAGCTTCATTACCATGA", "aa": "MYDIGVVPTKEPFYKVINQGMILGENNEKMSKSKGNVINPDDIIASHGADTLRIYEMFMGPLTASLP", "aa_hexdigest": "465a1d6a46a0429b3dc5455b6205cba3", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 498364, "stop": 498729, "observations": { "mutation": [ 498399, 498432, 498645, 498714 ], "stop": [ 498399, 498432, 498645, 498714 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0013027DF7", "query_cov": 0.9850746268656716, "subject_cov": 0.4520547945205479, "identity": 0.848, "score": 120.0, "evalue": 1.01e-33, "gene_start": 1, "gene_end": 66, "reference_start": 37, "reference_end": 102, "reference_sequence": "LASPEKLKKWLPVDVYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFMKLFNQGMILGENNEKMSKSKGNVDNPDDIVESHGADTLRLYEMFMGPLDASIAWSETGLDGARRFLDRVWRLFTNEDGSLSDKVTEQSGGALERSY" }, "paralog": false, "identity": 0.7520661157024794, "score": 187.0, "evalue": 2.36e-62, "description": "Internal stop codon at 498399, 498432, 498645, 498714. Nonsense mutation around 498399, 498432, 498645, 498714.", "psc": { "uniref90_id": "UniRef90_UPI0013027DF7", "product": "class I tRNA ligase family protein", "uniref50_id": "UniRef50_A0A359BCU9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0013027DF7", "UniRef:UniRef50_A0A359BCU9" ] } }, "id": "NKIECMJPJD_855", "locus": "GCA000828735_00842" }, { "type": "cds", "sequence": "contig_1", "start": 499036, "stop": 499293, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13603.12" ], "nt": "ATGAATTTTGCAACTGGAGCTGTTATGGGTGTACCAGCACATGATGAACGTGATTATGCTTTTGCTAAAAAATATGCATTACCAATTAAAAGTGTAATTGATACAAAACAAAAATTACCATATGCAGGTGATGGTTTACATATTAACTCAGCAATGATTAATGGTTTAAATATTAAACAATCTCAAAATGTTTTAAATGATTATTTAATTAAAAATCATCTAGGAAAAAAAGTTGCAAACTATAAATTGCGTAATTGA", "aa": "MNFATGAVMGVPAHDERDYAFAKKYALPIKSVIDTKQKLPYAGDGLHINSAMINGLNIKQSQNVLNDYLIKNHLGKKVANYKLRN", "aa_hexdigest": "b3dd206d459e8ed854000a70cf842c9a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13603.12", "name": "tRNA-synt_1_2", "length": 76, "aa_cov": 0.8, "hmm_cov": 1.0, "evalue": 1.249442374833758e-19, "score": 66.01909637451172, "start": 1, "stop": 68 } ], "seq_stats": { "molecular_weight": 9459.846799999992, "isoelectric_point": 9.69799518585205 }, "id": "NKIECMJPJD_856", "locus": "GCA000828735_00843" }, { "type": "cds", "sequence": "contig_1", "start": 499312, "stop": 499584, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13603.12" ], "nt": "GTGCAATTTAAGATTAAAGATTCACTTTTAACACTTGATGTGTTCACTACACGAATTGATACTATTTATGGGGTACAATACTTAGTAGTTGCACCAGAACATCCAATTCTTAAATCTATTACTAGTGAGCAACAAATTAATGTTGTTCAATCATATATTGAACAAACAAAGAAAATAAGCGATTTAGATCGCATTGCTGATACTAATAAAACTGGTGTTTTTAGTGGTGCATATGCTATTAATCCAATCAATCAAGAAATCATTCCAATTTGA", "aa": "MQFKIKDSLLTLDVFTTRIDTIYGVQYLVVAPEHPILKSITSEQQINVVQSYIEQTKKISDLDRIADTNKTGVFSGAYAINPINQEIIPI", "aa_hexdigest": "f0f91ebcae212ebd6b88e0458c2491e7", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13603.12", "name": "tRNA-synt_1_2", "length": 91, "aa_cov": 1.0, "hmm_cov": 0.978021978021978, "evalue": 1.0736983007128378e-25, "score": 85.79299926757812, "start": 1, "stop": 90 } ], "seq_stats": { "molecular_weight": 10154.55549999999, "isoelectric_point": 5.145486259460449 }, "id": "NKIECMJPJD_857", "locus": "GCA000828735_00844" }, { "type": "cds", "sequence": "contig_1", "start": 500053, "stop": 500175, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTCCATATCCTAGTGGAAAAGGATTGCATGTTGGTCATCCCAAAGGTTATACAGCAACTGATGTAATTAGTCGTTTTAAACGTTTGAATGGTTATGATGTTTTACATCCAATTGGTTGA", "aa": "MFPYPSGKGLHVGHPKGYTATDVISRFKRLNGYDVLHPIG", "aa_hexdigest": "64ecca8a855b65eb3eeedf0a466144ff", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4427.052000000001, "isoelectric_point": 9.696576881408692 }, "id": "NKIECMJPJD_858", "locus": "GCA000828735_00845" }, { "type": "cds", "sequence": "contig_1", "start": 500302, "stop": 500745, "strand": "-", "frame": 3, "gene": "ruvX", "product": "Holliday junction resolvase RuvX", "db_xrefs": [ "COG:COG0816", "COG:JLK", "EC:3.1.-.-", "EC:3.1.21.10", "GO:0000967", "GO:0004518", "GO:0005829", "GO:0006364", "RefSeq:WP_006688766.1", "SO:0001217", "UniParc:UPI00000C1C3F", "UniRef:UniRef100_Q9PQC1", "UniRef:UniRef50_Q9PQC1", "UniRef:UniRef90_Q9PQC1" ], "nt": "ATGCGTAAATTAGCACTTGATTTAGGAACAAAATCATGTGGTTTTGCAATTAGTGATTTATTAGGAATAATTGCTAGCGGACTTGATAATTTTATTTATGAAGAAAATGATTTTACAGCTGTTTTAGCTAAAATTGATGAAATCATGATTAATTATCATCATGAAATTGACACAATTGTTTTAGGTTATCCAACTAATGTTTATGATGGATCAAAAAATGAACGAACATATTTAATCGAATCATTTTATGCATTATTAAAACAACACTTTTTAAACCATGAAAAAATTAAAATTGTTTATGAGGATGAACGTTTTAGCACTAAAATTGCAACTCAACGTTTAAAAAATAGTTGTGTTAAAGCAGCTAAAATCAAAAAAGTTAAAGATAAAATGAGTGCTGTTGTTATTTTAGAAAGTTATTTAAGTAAAAATCATTTTAATTAA", "aa": "MRKLALDLGTKSCGFAISDLLGIIASGLDNFIYEENDFTAVLAKIDEIMINYHHEIDTIVLGYPTNVYDGSKNERTYLIESFYALLKQHFLNHEKIKIVYEDERFSTKIATQRLKNSCVKAAKIKKVKDKMSAVVILESYLSKNHFN", "aa_hexdigest": "9595dd16b96bdfcbab5ef58b05874f83", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI00000C1C3F", "ncbi_nrp_id": "WP_006688766.1", "uniref100_id": "UniRef100_Q9PQC1", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1C3F", "RefSeq:WP_006688766.1", "UniRef:UniRef100_Q9PQC1" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQC1", "uniref90_id": "UniRef90_Q9PQC1" }, "psc": { "uniref90_id": "UniRef90_Q9PQC1", "gene": "ruvX", "product": "Holliday junction resolvase RuvX", "ec_ids": [ "3.1.-.-", "3.1.21.10" ], "uniref50_id": "UniRef50_Q9PQC1", "cog_id": "COG0816", "cog_category": "JLK", "go_ids": [ "GO:0000967", "GO:0004518", "GO:0005829", "GO:0006364" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQC1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQC1" ], "product": "Putative pre-16S rRNA nuclease" }, "genes": [ "ruvX" ], "id": "NKIECMJPJD_859", "locus": "GCA000828735_00846" }, { "type": "cds", "sequence": "contig_1", "start": 501206, "stop": 501346, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTAACAGGTCATGATAATTTAGCAAAATACATTCATGATTTATATGTAGACTACTTAAATGAAATCAATCACTTAAAAGTTAATTTAGATATTAATGATCATAAATTAAATGATTTATATAATGCTTTTGCAAATTGA", "aa": "MVTGHDNLAKYIHDLYVDYLNEINHLKVNLDINDHKLNDLYNAFAN", "aa_hexdigest": "b89af7ef66a012b7bd590b0ccc1474f2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5414.969100000001, "isoelectric_point": 5.193401527404784 }, "id": "NKIECMJPJD_860", "locus": "GCA000828735_00847" }, { "type": "cds", "sequence": "contig_1", "start": 501356, "stop": 502000, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF07973.19" ], "nt": "TTGGTTGATGGTTTAGATCATGAAGATGGTTATGTTGTTTTTGATAACACATGTTTTTATGCAACATCAGGTGGTCAACAACATGACACAGGTTATATTATTAAAAATGATCAACAATTTTTTGTTGATGATGTTTTTAAGGCACCTAATCGTCAACACGTTCATCATGTTAAAAATGCTAGTTTATCAATGAATGAGCATGTTATTTTACAAATCAACGAGCAAGATCGTAAATCAATTACAGCAAACCATACAGCCGAACACTTATTACACTATTGCTTAAAACATGTTTTAAGTCCTGATATTAAACAAGAAGGAGCTGCTAAATATCCACATAAAGTAACATTTGATTTTACTTATCATGCTCAACCAACAAAAGCACAATTAGATAAGTTAGAAAATGTATTAAATGAAATGGTACAAAGTAATTTTGATGTACAAGAATTACACATGGATTTAGATGAAGCTAAAGCTGTTGGTGCTGCTGCTTATTTTGAAGATGTTTATAAAAAATTAAAAGGCAAATTACGTGTAATTAAAATGGGACCTTCAATTGAGTTATGTGGGGGTACACACGCACATCATACATCAGAAATCGAGCGAATTAAAATTGTTGAATGTGCTTCTAAAGGGGCTGGCTCTTGA", "aa": "MVDGLDHEDGYVVFDNTCFYATSGGQQHDTGYIIKNDQQFFVDDVFKAPNRQHVHHVKNASLSMNEHVILQINEQDRKSITANHTAEHLLHYCLKHVLSPDIKQEGAAKYPHKVTFDFTYHAQPTKAQLDKLENVLNEMVQSNFDVQELHMDLDEAKAVGAAAYFEDVYKKLKGKLRVIKMGPSIELCGGTHAHHTSEIERIKIVECASKGAGS", "aa_hexdigest": "5b0f9839d6b2a43fb8b460d4a426e744", "start_type": "TTG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07973.19", "name": "tRNA_SAD", "length": 39, "aa_cov": 0.17757009345794392, "hmm_cov": 1.0, "evalue": 3.9244508829845785e-13, "score": 44.776092529296875, "start": 176, "stop": 213 } ], "seq_stats": { "molecular_weight": 24114.864300000012, "isoelectric_point": 6.026604270935058 }, "id": "NKIECMJPJD_861", "locus": "GCA000828735_00848" }, { "type": "cds", "sequence": "contig_1", "start": 502043, "stop": 502423, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGTGATTTTTATACTTATTTAAAAGATGAAAAAACACTTACTAAAATTAAAATGATTTTAAATAAAGAAGTGCAATTATTTGAAAAAACTTTACAATTAGGTTTAAATATTTTTGAAAATTCTATTCGTAATCAAGAATTAGATAAAGAAATTACTTTTAAACTAGTTGATACATATGGTTTTCCAATTGAATTAATTAAAGAAATTTGTGAACAACGTAATGTTAAAGTTGATTTAGAAGCTTTTGATGCTATGTTTAAACATCACCAATTAATTTCAAAAGCAAACAAAGCTAATTTAAAAGTAATGGAATCACAAAACGAATCACTAATGCAATTAGATGTTGATTCAACCTTTCACTATGAAATATTTAGATGA", "aa": "MGDFYTYLKDEKTLTKIKMILNKEVQLFEKTLQLGLNIFENSIRNQELDKEITFKLVDTYGFPIELIKEICEQRNVKVDLEAFDAMFKHHQLISKANKANLKVMESQNESLMQLDVDSTFHYEIFR", "aa_hexdigest": "0f0e16c7f6e80f4a963438e17e5c6a73", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14957.109299999987, "isoelectric_point": 5.403762626647949 }, "id": "NKIECMJPJD_862", "locus": "GCA000828735_00849" }, { "type": "cds", "sequence": "contig_1", "start": 502457, "stop": 502768, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAAAGAATTGCTTGTATTTCACAAGATGTACCAACTAACTTTGATAGCGATGTTTTTATGCGAATTACAAAAAGTGTTGAACAGTTTTCAGAATATAAATATGACATGAATGAATACTTTCATCCCAATGTTACTCAAAATAAAATTAATTTCGCTTATAAAGTAATTGCAGATCATATGCGTGCAACTGTTTTTGCGATTGCAGATGGTGCAATTCCTTCAAATAAAGAACGAGGTTACATTTTACGTCGTTTAATTCGTCGAACTATGGTTTTAGTGCGTCGTTTAAATATTAACAATCTTTTATGA", "aa": "MERIACISQDVPTNFDSDVFMRITKSVEQFSEYKYDMNEYFHPNVTQNKINFAYKVIADHMRATVFAIADGAIPSNKERGYILRRLIRRTMVLVRRLNINNLL", "aa_hexdigest": "74994e3e4e343d0dcb6d545516f370c3", "start_type": "TTG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12147.918599999985, "isoelectric_point": 9.458753013610842 }, "id": "NKIECMJPJD_863", "locus": "GCA000828735_00850" }, { "type": "cds", "sequence": "contig_1", "start": 503489, "stop": 503725, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF07831.19" ], "nt": "ATGATTGCTGTTGATTTAAAAGCAGGACGTAAAGTTAAAACTGATCAATTAGATTTTCAAGCTGGAATTTATTTAAATAAGAAAAATAATGATTTCGTTAAAACAAATGAAGTTATTGCTACACTTTATGCTAGCGAGCCAATTAATGATGAAGTTGTTAATAAATACCATAACAATGTAGTTTATTTAAAAACACCAAAACAAATTGCACCATCAATTATTAAAGTAATGCGTTAA", "aa": "MIAVDLKAGRKVKTDQLDFQAGIYLNKKNNDFVKTNEVIATLYASEPINDEVVNKYHNNVVYLKTPKQIAPSIIKVMR", "aa_hexdigest": "998af4fca64e36ae0c62e02bc39e1849", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07831.19", "name": "PYNP_C", "length": 63, "aa_cov": 0.7948717948717948, "hmm_cov": 1.0, "evalue": 3.014899175599247e-23, "score": 76.89330291748047, "start": 1, "stop": 62 } ], "seq_stats": { "molecular_weight": 8879.224799999996, "isoelectric_point": 9.435479927062985 }, "id": "NKIECMJPJD_864", "locus": "GCA000828735_00851" }, { "type": "cds", "sequence": "contig_1", "start": 503852, "stop": 504790, "strand": "-", "frame": 2, "gene": "deoA", "product": "thymidine phosphorylase", "db_xrefs": [ "COG:COG0213", "COG:F", "EC:2.4.2.4", "GO:0006206", "SO:0001217", "UniRef:UniRef50_A0AAP9AA50", "UniRef:UniRef90_A0AAP9AA50" ], "nt": "ATGTTAAATGTTGTAGAAATTATTAGCAAGAAAAAAAATAATATTGAATTAAGTGAACAAGAAATTAAATTTGTTTATGATGGTTTTGTTAATAAGACAATTCCAGATTATCAAATGGCAGCTTTTTTAATGGCTGTTAATTTTTGTGGATATAGTGAAAATGAACAATACTATGCAACTAAAGCAATGGTTGAATCTGGTAAATTATTAGATTTGCGTGTTGAAAATAAAATTGTTGTTGACAAACATTCATCTGGTGGTGTGGGAGATAAAGTTTCAATTATTTTAACTCCTTTACTATCAGCTTTAGGATTATACGTTGGAAAAATGTCTGGTCGAGGATTAGGGCATACTGGTGGTACAGTTGATAAATTAGAATCATTGAATTTAAATTTAGATTTTGATTTAAAAACATACATGAATCAGCTAAAAGATAATGGTTTATTATTAACTGGTCAATCAGATGATATGGTTGTGGCTGATAAATATATTTATGCATTACGAGATGTAACAGCAACAAGTGATGTGTTTGATCTTATGATAGGATCAATTATGGCTAAAAAATTAGCTTTAATTACTGATTATATTTTCTTAGATGTTAAAGTTGGTGAAGGAGCATTTTGTAAAAATGTTGCACAAGCAGAATCTTTAGCAACAAAGATGTTAAAATTATCAAAGAAATTTAATCGTAATACAATTATCCATTTAACAAATATGGACCAACCTTTAGGAAAAGCAATTGGTAATGCTATTGAAATTAAAGAAAGTATGGATTATTTATTAGGTAAACCAGTGCCCCAAGATTTATATGATTTAATTAATCAGTTTGCTTTAGACATCTTAATTGATACAAAATTCGCTAAAAACAAGGAAGATGCTCAATCTAAGATTGATAACGTCATTAAAAATGGTTTAGCTTATCAAAAATTTGTGAATTGA", "aa": "MLNVVEIISKKKNNIELSEQEIKFVYDGFVNKTIPDYQMAAFLMAVNFCGYSENEQYYATKAMVESGKLLDLRVENKIVVDKHSSGGVGDKVSIILTPLLSALGLYVGKMSGRGLGHTGGTVDKLESLNLNLDFDLKTYMNQLKDNGLLLTGQSDDMVVADKYIYALRDVTATSDVFDLMIGSIMAKKLALITDYIFLDVKVGEGAFCKNVAQAESLATKMLKLSKKFNRNTIIHLTNMDQPLGKAIGNAIEIKESMDYLLGKPVPQDLYDLINQFALDILIDTKFAKNKEDAQSKIDNVIKNGLAYQKFVN", "aa_hexdigest": "297fef16f8dd0db89473250e1404dfd8", "start_type": "ATG", "rbs_motif": null, "genes": [ "deoA" ], "truncated": "5-prime", "pseudogene": { "start": 503549, "stop": 504790, "observations": { "mutation": [ 503845, 503854 ], "stop": [ 503845, 503854 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAP9AA50", "query_cov": 1.0, "subject_cov": 0.7188940092165899, "identity": 0.9740000000000001, "score": 595.0, "evalue": 1.26e-212, "gene_start": 1, "gene_end": 312, "reference_start": 1, "reference_end": 312, "reference_sequence": "MLNVVEIISKKKNNIELSEQEIKFVYDGFVNKTIPDYQMAAFLMAVNFCGYSENEQYYATKAMVESGKVLDLRVENKIVVDKHSSGGVGDKVSIILTPLLSALGLYVGKMSGRGLGHTGGTVDKLESLNLNLDFDLKTYMDQLKDNGLLLTGQSDDMVLADKYIYALRDVTATSDVFDLMVGSIMAKKLALITDYIFLDVKVGEGAFCKNVEQAESLATKMLKLSKKFNRNAIIHLTNMDKPLGKAIGNAIEIKESMDYLLGKPVPQDLYDLINQFALDILIDTKFAKNKEDAQAKIDNVIKNGLAYQKFVNWVRWWYKGDYISLENNTYFNPKYKLEILAKQDGYLDFKSTKELGMIAVDLKAGRKVKTDQLDFQAGIYLNKKNNDFVKTNEVIATLYANQPISDEVVNKYHNNVVYLKTPKQIAPSIIKVMR" }, "paralog": false, "identity": 0.961352657004831, "score": 781.0, "evalue": 1.82e-288, "description": "Internal stop codon at 503845, 503854. Nonsense mutation around 503845, 503854.", "psc": { "uniref90_id": "UniRef90_A0AAP9AA50", "gene": "DeoA", "product": "thymidine phosphorylase", "ec_ids": [ "2.4.2.4" ], "uniref50_id": "UniRef50_A0AAP9AA50", "cog_id": "COG0213", "cog_category": "F", "go_ids": [ "GO:0006206" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAP9AA50", "EC:2.4.2.4", "UniRef:UniRef50_A0AAP9AA50", "COG:COG0213", "COG:F", "GO:0006206" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAP9AA50", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAP9AA50" ], "product": "Thymidine phosphorylase" } }, "id": "NKIECMJPJD_865", "locus": "GCA000828735_00852" }, { "type": "cds", "sequence": "contig_1", "start": 505032, "stop": 505232, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAACTACAGCTAATAAAAAAATTAAATTAATTAATATTGAAAAATTAGAATTTGAAATTCTTGAGCCGATTAATACCAATGAGATTTTGGTTTTCAAAGATGAAAATTCAACTATTTATGAATCTTTTAATCAAAATTTAGAAAAACAACGTGATGAATTTATCAAAAAAATTCGTGAAGAAGAAGAACAAAATTGA", "aa": "MKTTANKKIKLINIEKLEFEILEPINTNEILVFKDENSTIYESFNQNLEKQRDEFIKKIREEEEQN", "aa_hexdigest": "933471465171ce7e97823a717c86c35d", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8002.967700000002, "isoelectric_point": 4.852367591857909 }, "id": "NKIECMJPJD_866", "locus": "GCA000828735_00853" }, { "type": "cds", "sequence": "contig_1", "start": 505566, "stop": 505706, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAATTATTAATTTTAAAGAAAGTAATGAATATCAAAAAATTATTAAAGATAAAATAGATTTAGATATTGAAATAGAAAAATTAAAATTTGCTATTCAAGCTCATGAAGATAATATGAAGGCTGCTAAAGAAAATTGA", "aa": "MAIINFKESNEYQKIIKDKIDLDIEIEKLKFAIQAHEDNMKAAKEN", "aa_hexdigest": "2f9c1b1d7265b184e74796f710546256", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5422.191000000001, "isoelectric_point": 5.21272678375244 }, "id": "NKIECMJPJD_867", "locus": "GCA000828735_00854" }, { "type": "cds", "sequence": "contig_1", "start": 505893, "stop": 506555, "strand": "+", "frame": 3, "gene": null, "product": "DUF2130 domain-containing protein", "db_xrefs": [ "COG:COG4487", "COG:S", "SO:0001217", "UniRef:UniRef50_A0A2C9DYH1", "UniRef:UniRef90_A0A2C9DYH1" ], "nt": "ATGACATTCACTAAAATTAATGAAGCAATTGATGGTAAAAAAGCTGACTTTTTATTAGAATTTTTTGATTTTGGTAAAGAAATGTCAAATGATGATAAAAAATTAATTTTTAGTGCGACAATTGAAGCGAAAACAGAATTTTTTGACAATCAAAAAGGTACAAAAAATAGTGCCCACTATAAAAAATTAGATCAAGATCGTATTAATCAAAAATCTGAATATGCAATTTTAGTAACTGAATTAGAACCAGAAGATCATTTTGTAATTAAAAAAATTAATGAATACAAAAATATGTTTGCTGTACGGCCACAATACTTCATTCCTTTAGTTGATATGATTCGAAATTTTGCAACCCTTAAAGCAAAAATTAATTCACAAATCATAAGATATGAAGATCGTGCAAAAATTGAAGAAAATCTTGATGAATTAAAAAAAGATATTGTTGATAACACTCTAAAATACATCAATGATAAAACTAAAAAAATAATCGATGATTCAAAAGCTATTATTAAAAAAGCAGAAAGTATTGAAGAAAGTGCTGAAGATATTATTAACAAAAAACTAAATACTTTAAAAAAGAAAATCAATGAATTAACAATTAGAAAAATTGATCCAAATAAACTTTTGTTAGAAGTTAGTGATGATGAAAATGATGAAGAATAA", "aa": "MTFTKINEAIDGKKADFLLEFFDFGKEMSNDDKKLIFSATIEAKTEFFDNQKGTKNSAHYKKLDQDRINQKSEYAILVTELEPEDHFVIKKINEYKNMFAVRPQYFIPLVDMIRNFATLKAKINSQIIRYEDRAKIEENLDELKKDIVDNTLKYINDKTKKIIDDSKAIIKKAESIEESAEDIINKKLNTLKKKINELTIRKIDPNKLLLEVSDDENDEE", "aa_hexdigest": "7457f97cfe341da8f41356e348b7f19d", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 505593, "stop": 506555, "observations": { "mutation": [ 505704, 505845 ], "stop": [ 505704, 505845 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A2C9DYH1", "query_cov": 1.0, "subject_cov": 0.4339250493096647, "identity": 1.0, "score": 335.0, "evalue": 1.08e-110, "gene_start": 1, "gene_end": 220, "reference_start": 288, "reference_end": 507, "reference_sequence": "MKTTANKKIKLINIEKLEFEILEPINTNEILVFKDENSTIYESFNQNLEKQRDEFIKKIREEEEQNWRNNFLANDRDYNELVKQRYDLEKQRDELKEKLKNEGNKAIAHFKDSDEYKNLIKAQEKINSLNKTIESNEQNYKKEIENIELKLKSQFDEETKSLKNTIAKQEIKLDNAEKMAIINFKESNEYQKIIKDKIDLDIEIEKLKFAIQAHEDNMKAAKENWESKKIVEIKELESKKDKEIHKLTESIEQLKREKSSNVKLVGEELEQWLKNKFDETYSFSCPDMTFTKINEAIDGKKADFLLEFFDFGKEMSNDDKKLIFSATIEAKTEFFDNQKGTKNSAHYKKLDQDRINQKSEYAILVTELEPEDHFVIKKINEYKNMFAVRPQYFIPLVDMIRNFATLKAKINSQIIRYEDRAKIEENLDELKKDIVDNTLKYINDKTKKIIDDSKAIIKKAESIEESAEDIINKKLNTLKKKINELTIRKIDPNKLLLEVSDDENDEE" }, "paralog": false, "identity": 0.99375, "score": 535.0, "evalue": 7.7e-192, "description": "Internal stop codon at 505704, 505845. Nonsense mutation around 505704, 505845.", "psc": { "uniref90_id": "UniRef90_A0A2C9DYH1", "product": "DUF2130 domain-containing protein", "uniref50_id": "UniRef50_A0A2C9DYH1", "cog_id": "COG4487", "cog_category": "S", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A2C9DYH1", "UniRef:UniRef50_A0A2C9DYH1", "COG:COG4487", "COG:S" ] }, "pscc": { "uniref50_id": "UniRef50_A0A2C9DYH1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2C9DYH1" ], "product": "DUF2130 domain-containing protein" } }, "id": "NKIECMJPJD_868", "locus": "GCA000828735_00855" }, { "type": "cds", "sequence": "contig_1", "start": 506695, "stop": 507171, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9X6V9", "UniRef:UniRef90_A0AAC9X6V9" ], "nt": "ATGTCTGCAACTAATAAAAAATATGATCCACAAATTAGCGAATTATTAGAGCTTCGTGATTTAGCTAATAAAGTTGATAATTTAAAAAGAAAATTAAAAACAATTGAATATAACCATTATTGTGAACACGAAAATTATGATAGTGATGATGGTGAAACTAATAATGTTTATGAACAAGAAGTGTCATTAAATCATGAAACACAAATTGTTAAATTACGTGTTGATTTAAATGATTTAACAAACCTAAGCAATACAAAAATTGTTGAAGTTGAAAAACCTGTTTATATTGAAAAAGTTATTGAAGTTGAAAAGCCAATCGAAAAAATTGTTGAGAAAATTGTTTATGTTAACCAAGATAATCAAGTTAATAAACAAGAAAGTATTAAAGAAGAAAAAGAACCAAAAACACCTGTTAAATCTGGCAAATACGTTAAATATCGTCGTTTAAAAAATAATGGTATGTCAGATGCTGGTTGA", "aa": "MSATNKKYDPQISELLELRDLANKVDNLKRKLKTIEYNHYCEHENYDSDDGETNNVYEQEVSLNHETQIVKLRVDLNDLTNLSNTKIVEVEKPVYIEKVIEVEKPIEKIVEKIVYVNQDNQVNKQESIKEEKEPKTPVKSGKYVKYRRLKNNGMSDAG", "aa_hexdigest": "6ec7f4ec6de758d34ea9cecd8df9c08c", "start_type": "ATG", "rbs_motif": "GGAGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 506683, "stop": 507318, "observations": { "mutation": [ 507169 ], "stop": [ 507169 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAC9X6V9", "query_cov": 1.0, "subject_cov": 0.7488151658767772, "identity": 1.0, "score": 295.0, "evalue": 3.83e-100, "gene_start": 1, "gene_end": 158, "reference_start": 5, "reference_end": 162, "reference_sequence": "MEEQMSATNKKYDPQISELLELRDLANKVDNLKRKLKTIEYNHYCEHENYDSDDGETNNVYEQEVSLNHETQIVKLRVDLNDLTNLSNTKIVEVEKPVYIEKVIEVEKPIEKIVEKIVYVNQDNQVNKQESIKEEKEPKTPVKSGKYVKYRRLKNNGMSDAGWKIFELVKQTQQDKVAAEKRFDEQQEEKKRQREQKRLEKQQKLEAKNEQ" }, "paralog": false, "identity": 0.990521327014218, "score": 377.0, "evalue": 8.46e-135, "description": "Internal stop codon at 507169. Nonsense mutation around 507169.", "psc": { "uniref90_id": "UniRef90_A0AAC9X6V9", "uniref50_id": "UniRef50_A0AAC9X6V9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAC9X6V9", "UniRef:UniRef50_A0AAC9X6V9" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAC9X6V9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9X6V9" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 18470.5683, "isoelectric_point": 5.5386983871459945 }, "id": "NKIECMJPJD_869", "locus": "GCA000828735_00856" }, { "type": "cds", "sequence": "contig_1", "start": 507894, "stop": 508106, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00152.25" ], "nt": "TTGTTTACAGGAAATGATGCTGAAGGAGCTGGCGAAACTTTTAGTATTCAAAAATTTGATAATGAAGAATTTTTTGGTAAACAAACTCATTTATCTGTAACAGGTCAATTACAAGCTGAAGCATACGCACAAGCTTTTGGTAATGTTTATACATTTGGTCCTACTTTTAGAGCTGAAAAATCTCACACAAATCGTCATTTAGCAGAATTTTGA", "aa": "MFTGNDAEGAGETFSIQKFDNEEFFGKQTHLSVTGQLQAEAYAQAFGNVYTFGPTFRAEKSHTNRHLAEF", "aa_hexdigest": "fda3433bcbf0d06af3dc5d60d28b2154", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00152.25", "name": "tRNA-synt_2", "length": 70, "aa_cov": 0.9714285714285714, "hmm_cov": 0.9285714285714286, "evalue": 7.563518839462494e-09, "score": 30.57632827758789, "start": 3, "stop": 70 } ], "seq_stats": { "molecular_weight": 7824.406400000001, "isoelectric_point": 5.131219673156737 }, "id": "NKIECMJPJD_870", "locus": "GCA000828735_00857" }, { "type": "cds", "sequence": "contig_1", "start": 508107, "stop": 508631, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00152.25" ], "nt": "ATGATTGAACCTGAAATGGCTTTTGTTGATTTAAAAGGTATGCAAGATATTGTTGAAGATTTAGTTAAACATGTTATTAAGGCTGTTTTAGAAAAAAATCAACAAGAATTAGAATTTTTAGCTCAAAGAAATGATGAAAATTTAATTAAAAAACTACAAAAAGTTGTTGAGTCTAAATTTGAACGTATTGAATATAAAGATGCAGTTAAAATCCTAGCTGATGCTGTTAAAAATGGTCATCAATTCGAAAATAATGAAATCTTTTTTGGAATGGATTTAGGATCAGAGCACGAAAGATACATGTGTGAAACATATCATCAAGGTCCTGTGTTCTTACAAAATTATCCAAAGGATATTAAAGCATTTTATATGAAATTAAACGATGATCAACAAACTGTAGCTTCAACAGATTTATTAATTCCAGGCGTTGGCGAATTAGTGGGTGGATCACAACGTGAAGATAGTTATGAGAAACTATTAAAACGATGTCAAGAATTAAAAATGCCAATTGAAAGTTTACAATGA", "aa": "MIEPEMAFVDLKGMQDIVEDLVKHVIKAVLEKNQQELEFLAQRNDENLIKKLQKVVESKFERIEYKDAVKILADAVKNGHQFENNEIFFGMDLGSEHERYMCETYHQGPVFLQNYPKDIKAFYMKLNDDQQTVASTDLLIPGVGELVGGSQREDSYEKLLKRCQELKMPIESLQ", "aa_hexdigest": "a64464f5150cb50f2f10fd3f2e8561a5", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00152.25", "name": "tRNA-synt_2", "length": 177, "aa_cov": 0.9655172413793104, "hmm_cov": 0.7909604519774012, "evalue": 1.3919136002115223e-26, "score": 88.824462890625, "start": 1, "stop": 168 } ], "seq_stats": { "molecular_weight": 20199.898899999997, "isoelectric_point": 4.9362619400024395 }, "id": "NKIECMJPJD_871", "locus": "GCA000828735_00858" }, { "type": "cds", "sequence": "contig_1", "start": 508662, "stop": 508775, "strand": "+", "frame": 3, "gene": null, "product": "Aminoacyl-tRNA synthetase class II (D/K/N) domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A4Q3ETT1" ], "nt": "ATGTCATCAGGTTTTGGAATTGGTTTTGAGCGTTTAGTGATGTATGTAACAGGAGTTAATAATATTAAAGATACAATCCCATTCCCACGAAGTCATGGCCAAATTGAATTTTAA", "aa": "MSSGFGIGFERLVMYVTGVNNIKDTIPFPRSHGQIEF", "aa_hexdigest": "7edc3b4addcd3396cfc476fa07e55789", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_A0A7X8F0W0", "query_cov": 0.9459459459459459, "subject_cov": 0.813953488372093, "identity": 0.6, "score": 53.1, "evalue": 1.17e-08, "valid": false, "product": "Aminoacyl-tRNA synthetase class II (D/K/N) domain-containing protein", "uniref50_id": "UniRef50_A0A4Q3ETT1" }, "pscc": { "uniref50_id": "UniRef50_A0A4Q3ETT1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A4Q3ETT1" ], "product": "Aminoacyl-tRNA synthetase class II (D/K/N) domain-containing protein" }, "genes": [], "id": "NKIECMJPJD_872", "locus": "GCA000828735_00859" }, { "type": "cds", "sequence": "contig_1", "start": 508756, "stop": 509286, "strand": "+", "frame": 1, "gene": "pgsA", "product": "CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase", "db_xrefs": [ "EC:2.7.8.5", "GO:0008444", "GO:0008654", "GO:0016020", "KEGG:K00995", "SO:0001217", "UniRef:UniRef50_Q9PQC7", "UniRef:UniRef90_Q9PQC7" ], "nt": "ATGGCCAAATTGAATTTTAAAAAAAACATCTCATCATCACCTTTTTTTAGAAACATTCCTAATATTATCACTATCTTTAGAATTTTTTTAGCACTTATTTGCATCATCTTATTATTAGTTGATTTTTACACAAAAAATTTAAATATTGTCTATGAAGTTTTAGATGCCAATATTAGTGCATTGCGTTTAAGTGCAACAATTATTTTTATTATTGCTGCTTTTTCTGATTTCTTAGATGGATATATAGCTCGTAAATATAATTTAGTTTCAAATTTAGGGAAAATCTTAGATCCTATTTCTGATAAAATCCTTGTTAATGGTGTTTTAATTTGTTTAACACTAGATCATACAGCACTAGCTTATTTAACAATTATTAATATCTTACGAGATATTTTTATTGATGGTTTACGAATGTTTGCTTCAAGTAAGAAAATTATTATTCCAGCAAATATTTTTGGCAAAATTAAATCCATTCTACTATTTATTAGTATTTGCTTTATTTTATTTTTACTAAGCTTAACCTCATCATGA", "aa": "MAKLNFKKNISSSPFFRNIPNIITIFRIFLALICIILLLVDFYTKNLNIVYEVLDANISALRLSATIIFIIAAFSDFLDGYIARKYNLVSNLGKILDPISDKILVNGVLICLTLDHTALAYLTIINILRDIFIDGLRMFASSKKIIIPANIFGKIKSILLFISICFILFLLSLTSS", "aa_hexdigest": "0e5a90ebcfb42603dac8c9e1b6d3a23d", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_Q9PQC7", "query_cov": 1.0, "subject_cov": 0.8073394495412844, "identity": 0.983, "score": 312.0, "evalue": 2.01e-106, "valid": true, "gene": "pgsA", "product": "CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase", "ec_ids": [ "2.7.8.5" ], "uniref50_id": "UniRef50_Q9PQC7", "kegg_orthology_id": "K00995", "go_ids": [ "GO:0008444", "GO:0008654", "GO:0016020" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQC7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQC7" ], "product": "CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase" }, "genes": [ "pgsA" ], "id": "NKIECMJPJD_873", "locus": "GCA000828735_00860" }, { "type": "cds", "sequence": "contig_1", "start": 509390, "stop": 509626, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03590.20" ], "nt": "GTGGATCAATTACAAAAAGCTAAAATTAATCAAACACAAAAAGCAATTGTAGAGATTAAAAATTCTTTTCAAAAACATTTTGCAAAAAATTTAAATCTTTCACGTGTAACAGCACCATTATTTGTTGAAGGACAATCAGGTCTAAATGATCATTTAGACCATAAACAAAAAGCAGTAAGTTTTTATGCTAAAAAATTAGATAAAACACTTGAAATTGTTCAATCACTAGCAAAATGA", "aa": "MDQLQKAKINQTQKAIVEIKNSFQKHFAKNLNLSRVTAPLFVEGQSGLNDHLDHKQKAVSFYAKKLDKTLEIVQSLAK", "aa_hexdigest": "ea6671d2eba639b41fd7de58d1fe233c", "start_type": "GTG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03590.20", "name": "AsnA", "length": 67, "aa_cov": 0.8461538461538461, "hmm_cov": 1.0, "evalue": 5.704248876212226e-21, "score": 70.86546325683594, "start": 13, "stop": 78 } ], "seq_stats": { "molecular_weight": 8864.154199999994, "isoelectric_point": 9.795277976989745 }, "id": "NKIECMJPJD_874", "locus": "GCA000828735_00861" }, { "type": "cds", "sequence": "contig_1", "start": 510125, "stop": 510328, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGAATTCGTGTTAGCAAACAAGATTTTATTAAGCAAACAAATTTTGCTAATTTAAAAAATGATCAAGAAAATAATTTTTATCATCAAATGATTTTAAATAATCAACTACCCCAAACAATTGGTGGGGGAATCGGTCAAAGTCGTTTATGTATGTTTTTATTAAACAAAAAACACATTGGTGAGGTACAAGTTTCAGTTTGA", "aa": "MGIRVSKQDFIKQTNFANLKNDQENNFYHQMILNNQLPQTIGGGIGQSRLCMFLLNKKHIGEVQVSV", "aa_hexdigest": "edb95d7efde8b4eaaecf5bcfae9c6cb6", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI000314F64C", "query_cov": 1.0, "subject_cov": 0.8170731707317073, "identity": 0.552, "score": 69.3, "evalue": 4.09e-14, "valid": false, "uniref50_id": "UniRef50_A0A352WUX0" }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7648.739100000001, "isoelectric_point": 9.42909755706787 }, "id": "NKIECMJPJD_875", "locus": "GCA000828735_00862" }, { "type": "cds", "sequence": "contig_1", "start": 510411, "stop": 511538, "strand": "+", "frame": 3, "gene": "adhE", "product": "NADP-dependent glyceraldehyde-3-phosphate dehydrogenase", "db_xrefs": [ "COG:COG1012", "COG:I", "SO:0001217", "UniRef:UniRef50_Q9PQC9", "UniRef:UniRef90_Q9PQC9" ], "nt": "TTGAACTATAAAACATTAATTAATGGAGCATTTGTTGATGCTAAAGAAAAACTACCAGTTTATAATCCATCTAATAATCAAATTATTGCTTATGTTCCTAACATTCATAATGAAAATGAAATTAATACTATTTTTGAAAATGCTCATATAGCATATCTAAAATTTAAGGACACACCAATTAAATATCGTTGTGATCTACTTTTAAAATTAGCCGATAAACTAGATGAACATAAACAAGAATTAGCACAAATTATTAGTACTGAAATTGCAAAAGGATTAAAAGATAGCCTAATTGAAGTTGAACGAAGTGCTGATTATTTACGCGAAACAGTTTTTGAGTATCAAAAATTAATGCAAAAACCAATTATTTTTGATGAAACAGTTCACCATGTTAAAAACAAAGTGGCAACATATTATCGTATTCCAGTTGGTGTTGTTTTAGCAATTTGTCCTTTTAATTATCCTATTAACTTATTGATTTCAAAATTAGCACCAGCATTAGTTTCTGGTAATAGTTTAGTTTATAAACCATCAACACAAGGTTCATTAATTGGTATTCGAATTAGTGAATTAGTTCATGAAGTAGGTTTTCCAAAAGGAGTTGTTAACTGCTTAACAACTGAAGCACGTATAACAGGTGATCTACTAGTTACTAATAAATATGTTAAAGCGATTAGCTTTACGGGCGGACCTAAAGTAGGCAATCATATTGCTGAAATAACATCAAAGATTTCTTTAGTTTTAGAATTAGGAGGAAAAGATCCAGCCCTTGTTTTAGATGATGCGGATTTTGAACTAGCTGCTAATGAAATAGTTAAAGGTGCTTATGGCTTTAGCGGACAAAGATGTACTGCAATCAAACGAGTATTTGTTTCGCATAAAAATCATGATTTATTAGTTAATTTAATTAACAAAAAAGTTGACGCTTTAACAGTCGGTTTACCACAACAAAATCCCATAATTACTCCATTAATTAATTCTAATTCATTAAAATATAACTTATCATTAGTTGAGGATGCAATCAAAAAAGGAGCAATAGTACACCAAAAAATCGTTTATAATGAAAAAAATAACCTTTTGCATCCTTTAGTAATTGATAATGTTACAAAAGAAATGCGCGTAGCTTGA", "aa": "MNYKTLINGAFVDAKEKLPVYNPSNNQIIAYVPNIHNENEINTIFENAHIAYLKFKDTPIKYRCDLLLKLADKLDEHKQELAQIISTEIAKGLKDSLIEVERSADYLRETVFEYQKLMQKPIIFDETVHHVKNKVATYYRIPVGVVLAICPFNYPINLLISKLAPALVSGNSLVYKPSTQGSLIGIRISELVHEVGFPKGVVNCLTTEARITGDLLVTNKYVKAISFTGGPKVGNHIAEITSKISLVLELGGKDPALVLDDADFELAANEIVKGAYGFSGQRCTAIKRVFVSHKNHDLLVNLINKKVDALTVGLPQQNPIITPLINSNSLKYNLSLVEDAIKKGAIVHQKIVYNEKNNLLHPLVIDNVTKEMRVA", "aa_hexdigest": "0b4a759e40e44648432dada4dcf8caa6", "start_type": "TTG", "rbs_motif": "AGGAG", "genes": [ "adhE" ], "truncated": "3-prime", "pseudogene": { "start": 510411, "stop": 511838, "observations": { "mutation": [ 511536 ], "stop": [ 511536 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQC9", "query_cov": 1.0, "subject_cov": 0.7894736842105263, "identity": 0.981, "score": 717.0, "evalue": 5.47e-259, "gene_start": 1, "gene_end": 375, "reference_start": 1, "reference_end": 375, "reference_sequence": "MNYKTLINGAFVDAKEKLAVYNPSNNQIIAYVPNIHDENEINTIFENAHKAYLKFKDTTIECRCDLLLKLADKLDKHKQELAQIISTEIAKGLKDSLIEVERSADYLRETVFEYQKLMQKPIIFDETVHHVKNKVATYYRIPVGVVLAICPFNYPINLLISKLAPALVSGNSLVYKPSTQGSLIGIRISELVHEVGFPKGVVNCLTTEARITGDLLVTNKYVKAISFTGGPKVGNHIAEITSKISLVLELGGKDPALVLDDADFELAANEIVKGAYGFSGQRCTAIKRVFVSHKNHDLLVNLINKKVDALTVGLPQQNPIITPLINSNSLKYNLSLVEDAIKKGAIVHQKIVYNEKNNLLHPLVIDNVTKEMRVAWEEPFGPILPIITYNSIQEAIDLINQSQYGLQACIFTTNYASIEQLALQIESGTININKSSSRGPDILPFFGVKDSGFGIQGIVDAILSMTTIKGIIINK" }, "paralog": false, "identity": 0.9789473684210527, "score": 910.0, "evalue": 0.0, "description": "Internal stop codon at 511536. Nonsense mutation around 511536.", "psc": { "uniref90_id": "UniRef90_Q9PQC9", "gene": "AdhE", "product": "NADP-dependent glyceraldehyde-3-phosphate dehydrogenase", "uniref50_id": "UniRef50_Q9PQC9", "cog_id": "COG1012", "cog_category": "I", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQC9", "UniRef:UniRef50_Q9PQC9", "COG:COG1012", "COG:I" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQC9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQC9" ], "product": "NADP-dependent glyceraldehyde-3-phosphate dehydrogenase" } }, "id": "NKIECMJPJD_876", "locus": "GCA000828735_00863" }, { "type": "cds", "sequence": "contig_1", "start": 511880, "stop": 512071, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCGCGCATTACCTGTACAAGTTTTAAGTAATAATAAATATGTTGATATTTACGTAAAGATTCCTGTTTTATTAGCTAATACTAAAATGTTAGTTGTTGGTGATGATTTACTAAAATCATTTTATTTTGATACAGATAACTTAATAATGAATGCTAAACATTTTCAACCAATTCCTATCAAAATGCTTTAG", "aa": "MRALPVQVLSNNKYVDIYVKIPVLLANTKMLVVGDDLLKSFYFDTDNLIMNAKHFQPIPIKML", "aa_hexdigest": "01708b12d56e12c70f4df3142a577e7b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7251.6391, "isoelectric_point": 9.100502204895019 }, "id": "NKIECMJPJD_877", "locus": "GCA000828735_00864" }, { "type": "cds", "sequence": "contig_1", "start": 512171, "stop": 512404, "strand": "-", "frame": 2, "gene": null, "product": "Lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T0Z0", "UniRef:UniRef90_A0AAC9T0Z0" ], "nt": "ATGAAAAATAAGTATTTTAGAAAAAATAAATTTTGGTTATTAATACCAATAAGTTTTGTAAGTCTTGGAAGTATTACATTAGTTGCTACTGCTTGTGTAAAGAAAAATTTTTATCCTAATACATTTGCAGATGATCAGATTTATATCACTAAGAATAATGAAAAAACTGTTAAGTTTCGTATTAGTCGTAATGATGAAGAAAATAAAAAGTATTTTAAAGATGATCTTAAATGA", "aa": "MKNKYFRKNKFWLLIPISFVSLGSITLVATACVKKNFYPNTFADDQIYITKNNEKTVKFRISRNDEENKKYFKDDLK", "aa_hexdigest": "20837a7943271dc80082b53a6b299f09", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 511880, "stop": 512404, "observations": { "mutation": [ 512173 ], "stop": [ 512173 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAC9T0Z0", "query_cov": 1.0, "subject_cov": 0.4425287356321839, "identity": 0.935, "score": 146.0, "evalue": 2.31e-43, "gene_start": 1, "gene_end": 77, "reference_start": 1, "reference_end": 77, "reference_sequence": "MKNKYFRKSKFWLLIPISFIXXXSITLVATACVKKNFYPNTFADDQIYITKNNEKTVKFRISRNDEENKKYFKDDLKWNTFINKLKKTYINVETVIPKENAKQNIKQQDLCMRALPVQVLSNNKYVDIYVKIPVLLANTKMLVVGDDLLKSFYFDTDNLIMNAKHFQPIPIKML" }, "paralog": false, "identity": 0.9655172413793104, "score": 339.0, "evalue": 6.32e-122, "description": "Internal stop codon at 512173. Nonsense mutation around 512173.", "psc": { "uniref90_id": "UniRef90_A0AAC9T0Z0", "product": "Lipoprotein", "uniref50_id": "UniRef50_A0AAC9T0Z0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAC9T0Z0", "UniRef:UniRef50_A0AAC9T0Z0" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAC9T0Z0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T0Z0" ], "product": "Lipoprotein" } }, "id": "NKIECMJPJD_878", "locus": "GCA000828735_00865" }, { "type": "cds", "sequence": "contig_1", "start": 512562, "stop": 512678, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGGTGAAAATAGTCCAGAAGTTGATTTGGGTACTCCTAATTTTACTAAGGATAATGTTAAACATTTACGTGCTTTTAGAGATGAATTAAATGTTAAAAATACTGATGAATAA", "aa": "MNGENSPEVDLGTPNFTKDNVKHLRAFRDELNVKNTDE", "aa_hexdigest": "7b04eebe2de077b3f1328567f83a9b9a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4345.6734000000015, "isoelectric_point": 4.864417457580566 }, "id": "NKIECMJPJD_879", "locus": "GCA000828735_00866" }, { "type": "cds", "sequence": "contig_1", "start": 512679, "stop": 512981, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGGTGAAGCTTTTGAAGGAACGGTTGATAAAGTAATTTATTCATATAATGAATATTTAATTAAAGGATATAAACCAGGAACAGCAGAAGGTGAAGCTGTAATTAAAAAATTCGAAGCTAATTTAGCAAAATATGTAAATAGTTTAGATATAAATACAAAAAATAGTTTTAATCCTACAAGGATTTTAAAAGAAAAGCCAGTTGTTGGTAAAAATTTCTTTATTGAACGTGAAGGAACATTCTGCGATGGTTGTTATGGTTTTATGGGTCAAAAAACAATTGTTAATATTTTAAATCGTTGA", "aa": "MGEAFEGTVDKVIYSYNEYLIKGYKPGTAEGEAVIKKFEANLAKYVNSLDINTKNSFNPTRILKEKPVVGKNFFIEREGTFCDGCYGFMGQKTIVNILNR", "aa_hexdigest": "96237c3c2d659c97e8df4518dd6d31c5", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11286.811299999994, "isoelectric_point": 8.51383991241455 }, "id": "NKIECMJPJD_880", "locus": "GCA000828735_00867" }, { "type": "cds", "sequence": "contig_1", "start": 513054, "stop": 513269, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTAGTCCTGATGTAATGCCAAATGGTCGAATTGTAGATCCAAACATTACTAAAGAAGATTTATTACATTTAAGTAATTTTTTTATTACCCAACCCGTTTATTTACCAATTTTATATGCTGATCAAAATCATCCTTATACACCTGGAATGGGTTGTAAATTCCCTATTCCTAAAAAACACTTAGAAGATGTACAAAAATATATTGATAATTGA", "aa": "MISPDVMPNGRIVDPNITKEDLLHLSNFFITQPVYLPILYADQNHPYTPGMGCKFPIPKKHLEDVQKYIDN", "aa_hexdigest": "305fa4d6dffa1b9915d154a83a15dbd2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8182.386199999999, "isoelectric_point": 5.773841285705566 }, "id": "NKIECMJPJD_881", "locus": "GCA000828735_00868" }, { "type": "cds", "sequence": "contig_1", "start": 513309, "stop": 513746, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATTTCACAGGTAAAGGAGCATTACCAGAGAATTTTGCTAAATTAAATGTGGGTACATTATTTATTGCTGATTTTAAATTCAATAAGTATAAAGAAAATTTTTTAGATAAAAATGAAATTGGTGGTGTAGCAATGCAATCACGATCATCAGGTTTGCCTTCAAAAACACCAGCCTTATTCATGCCTAGCTGGTTGTTTGGTAAAAATAATCCTTATTTAGAAACTGATGTTAATGCTTCAGCTGGAATTACAGATTTTTTTGTTGATCCATATGATGGCTTAATCTTTACAGGTAAAACCTTAGATCGCATTTATGATGCAAAAAAATTTGATAATGTTAATAAAGCGGTTATTGATGGTAAAAGCAATTTTCAAACATTTACAGATTATGCTAATGTTGCTGTAAAAGCTGTTCGAAAAAGAGTTTCACAATGA", "aa": "MNFTGKGALPENFAKLNVGTLFIADFKFNKYKENFLDKNEIGGVAMQSRSSGLPSKTPALFMPSWLFGKNNPYLETDVNASAGITDFFVDPYDGLIFTGKTLDRIYDAKKFDNVNKAVIDGKSNFQTFTDYANVAVKAVRKRVSQ", "aa_hexdigest": "679c2e3fa5ba039b8d5b8cf6c9c6719e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16143.15389999998, "isoelectric_point": 9.246458625793458 }, "id": "NKIECMJPJD_882", "locus": "GCA000828735_00869" }, { "type": "cds", "sequence": "contig_1", "start": 514023, "stop": 514196, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGTTGAAGTTTAAAAAAATCTTATTATCTATATCAACTAGTTTAATAATTTTATCATCAATTATTACAATTGCTGCATGTAGCAATCAAGTAAAATCTAAAACAAATTTTTCAAAAACAACACCAACTTTAGAAGAATTGACTGAGCTTAAAAAAAGAGAAGAAGCATGA", "aa": "MKLKFKKILLSISTSLIILSSIITIAACSNQVKSKTNFSKTTPTLEELTELKKREEA", "aa_hexdigest": "99ba6db3745f8d2b7a036199857b7604", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6369.532199999998, "isoelectric_point": 9.731776618957518 }, "id": "NKIECMJPJD_883", "locus": "GCA000828735_00870" }, { "type": "cds", "sequence": "contig_1", "start": 514289, "stop": 514405, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGGTGAAAATAGTCCAGAAGTTGATTTGGGTATTCCTAATTTTACTAAGGATAATGTTAAACATTTACGTGCTTTTAAAGATGAATTAAATGTTAAAAATACTGATGAATAA", "aa": "MNGENSPEVDLGIPNFTKDNVKHLRAFKDELNVKNTDE", "aa_hexdigest": "55e052b34c7ff6e3ace59dff2e0ac26d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4329.713700000001, "isoelectric_point": 4.864417457580566 }, "id": "NKIECMJPJD_884", "locus": "GCA000828735_00871" }, { "type": "cds", "sequence": "contig_1", "start": 514406, "stop": 514708, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGGTGAAGCTTTTGAAGGAACGGTTGATAAAGTAATTTATTCATATAATGAATATTTAATTAAAGGATATAAACCAGGAACAGCAGAAGGTGAAGCTATAATTAAAAAATTCGAAGCTAATTTAGCAAAATATGTAAATAGTTTAGATATAAATACAAAAAATAGTTTTAATCCTACAAGGATTTTAAAAGAAAAGCCAGTGGTAGGTAAAAACTTTTTTATCGAAAGAAAAGGTAATATTTACGATGCTAATTATGGATTTATAGGTCAAAAAACTATGGTTAATATTTTAAATCGTTGA", "aa": "MGEAFEGTVDKVIYSYNEYLIKGYKPGTAEGEAIIKKFEANLAKYVNSLDINTKNSFNPTRILKEKPVVGKNFFIERKGNIYDANYGFIGQKTMVNILNR", "aa_hexdigest": "13aee8182c833eb50b9cd42c2f6a97f5", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11363.895299999991, "isoelectric_point": 9.272955131530761 }, "id": "NKIECMJPJD_885", "locus": "GCA000828735_00872" }, { "type": "cds", "sequence": "contig_1", "start": 514778, "stop": 514993, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAATGCCAAACGTAATGAAAGGAGCACGAATTATTGATCCTAATTTTAGTAAAGATGATTTACTATTCTTAGGTAATATTTTTATTCCACAACCTGTTTATTTTCCAATTTTATATGCAGACCAAAATGATGAATATACACCTGGATTAGGAGCTAAATTTCCTATTCCTAAAAAAGATATAAATGAAGTTCAACGCTATGTAGATAATTGA", "aa": "MTMPNVMKGARIIDPNFSKDDLLFLGNIFIPQPVYFPILYADQNDEYTPGLGAKFPIPKKDINEVQRYVDN", "aa_hexdigest": "3e0284f79302b7f13b5f79c4c7f10c41", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8148.301800000003, "isoelectric_point": 4.717147636413574 }, "id": "NKIECMJPJD_886", "locus": "GCA000828735_00873" }, { "type": "cds", "sequence": "contig_1", "start": 515033, "stop": 515335, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAGTCACGATCATCAGGTTTGCCTTCAAAAACACCAGCTTTATTCATGCCTAATTGGTTATTTGGTAAAAATAACCCTTATTTAGAGACTGACGTTAACGCTTCAGCTGGAATTACAGATTTTTTTGTTGATCCATATGATGGCTTAATCTTTACAGGTAAAACCTTAGATCGCATTTATGATGCAAAAAAATTTGATAATGTTAATAAAGCGGTTATTGATGGTAAAAGCAATTTTCAAACATTTACAGATTATGCAAAAGCAATTGTTAGTGTTGTACGAAAGAATGTTTCGAATTGA", "aa": "MQSRSSGLPSKTPALFMPNWLFGKNNPYLETDVNASAGITDFFVDPYDGLIFTGKTLDRIYDAKKFDNVNKAVIDGKSNFQTFTDYAKAIVSVVRKNVSN", "aa_hexdigest": "9528ec6722b35e77d9e4ee6b6fa00c8b", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11134.446999999991, "isoelectric_point": 8.92521266937256 }, "id": "NKIECMJPJD_887", "locus": "GCA000828735_00874" }, { "type": "cds", "sequence": "contig_1", "start": 515362, "stop": 515469, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAACTTAAAGGTGTTAAAGCAGAAACATTAGCTGCTGAGAATGTAGGTACTTTATTTAATTTATTGCAGATTTTAAATTTGGAAATTATAAAACTAATTTTTTAG", "aa": "MELKGVKAETLAAENVGTLFNLLQILNLEIIKLIF", "aa_hexdigest": "5b4fd67fa4d0de55e48430d695466c04", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3900.6661000000004, "isoelectric_point": 4.944674110412596 }, "id": "NKIECMJPJD_888", "locus": "GCA000828735_00875" }, { "type": "cds", "sequence": "contig_1", "start": 515810, "stop": 515998, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCATTTGAAGACTTAACTTCATTATTAACTTTACTACAAGCTGTAGTTGTACTTACAATAGTTGTTATAAAAGCAATAGAAGCTATTGAAGTAATTCAAATTTTTTTATATTTCATAACAATATTCTTTCTTTATTTTTTATTTAAAAAAATTATTAATACCTTTAATTTTAATACACAAAAATAA", "aa": "MSFEDLTSLLTLLQAVVVLTIVVIKAIEAIEVIQIFLYFITIFFLYFLFKKIINTFNFNTQK", "aa_hexdigest": "65b8a3374a31e4d7a68460a9e3ed6c2a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7280.727999999995, "isoelectric_point": 6.013133430480956 }, "id": "NKIECMJPJD_889", "locus": "GCA000828735_00876" }, { "type": "cds", "sequence": "contig_1", "start": 516078, "stop": 516455, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCGTTCTGCTAAATTTAAAGTTGTTATTACAGATACAATGATTGCTGAACAATTAGGATTAAAAGTAAAATTATTGAATTTAGAAATGATTTTTATCTCATCTTGTTTAACAGCTGTTTCATATATTATGGGAGGAAATGTATTATTTTTAGGAATTGCTGCAGCAAATTTAGGGTATGCGGTTTTTGGTAATCGCTTTAGTTCATCAATAATTTTTTCAAGCTTCATCACCATTATCTTTTTAATTTTTGGTCAATTTATTTTAGATAATATCGTTCAAAATTTAACTAGTTTGGTATTTGACGCCCCTATTGTAACTCCTTTATTAGTAGCTCCAATATTTACATTTATTATCTTATTTAAAAATAATATTTAA", "aa": "MRSAKFKVVITDTMIAEQLGLKVKLLNLEMIFISSCLTAVSYIMGGNVLFLGIAAANLGYAVFGNRFSSSIIFSSFITIIFLIFGQFILDNIVQNLTSLVFDAPIVTPLLVAPIFTFIILFKNNI", "aa_hexdigest": "2458d155957c8089e6e453be5cc43da2", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13747.385399999977, "isoelectric_point": 8.930628013610843 }, "id": "NKIECMJPJD_890", "locus": "GCA000828735_00877" }, { "type": "cds", "sequence": "contig_1", "start": 516577, "stop": 516753, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTCTTTTAAGCTAATTGATAAAGCAATAGCAATAAAATTAATGAATACACCAGAGACAATTAACTTTGTATGGCTAAAACCGGTTCGTTTTTTTGAAAATCAATACAAAATTAATCCGGCACATACTCCTGATATTATAAAAATAAAAGGTAATAAATTTTCAAAAAGTTTTTGA", "aa": "MSFKLIDKAIAIKLMNTPETINFVWLKPVRFFENQYKINPAHTPDIIKIKGNKFSKSF", "aa_hexdigest": "01e2e81074753b1df8607953180071f6", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6813.038900000001, "isoelectric_point": 9.998482322692869 }, "id": "NKIECMJPJD_891", "locus": "GCA000828735_00878" }, { "type": "cds", "sequence": "contig_1", "start": 516678, "stop": 516872, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTCCAACGGATTACTAAAAATCGATTAGTTGATACATCAACTCTTGGGATAGGTAATGTTTGTTTGATTGGGTTATTTTTTTTAGCAATATCAATTAATTTTGAAAATTATGGTTCTCAAAAACTTTTTGAAAATTTATTACCTTTTATTTTTATAATATCAGGAGTATGTGCCGGATTAATTTTGTATTGA", "aa": "MFQRITKNRLVDTSTLGIGNVCLIGLFFLAISINFENYGSQKLFENLLPFIFIISGVCAGLILY", "aa_hexdigest": "e55ef750bd793d1e1cd7e992170cafd4", "start_type": "ATG", "rbs_motif": "AGGAG/GGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7161.449999999996, "isoelectric_point": 7.789859962463378 }, "id": "NKIECMJPJD_892", "locus": "GCA000828735_00879" }, { "type": "cds", "sequence": "contig_1", "start": 516906, "stop": 517001, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTATTAAAATTAATAACCAAGGTCAATTATTAAATAATCGGACTCCTTTAGATTTGTTTAAAGTAAATTATGAAACAATCTTACAAATTTGA", "aa": "MFIKINNQGQLLNNRTPLDLFKVNYETILQI", "aa_hexdigest": "63b31c385971f9c88c5886832a9acb23", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3692.2881000000007, "isoelectric_point": 8.253774452209473 }, "id": "NKIECMJPJD_893", "locus": "GCA000828735_00880" }, { "type": "cds", "sequence": "contig_1", "start": 517044, "stop": 517181, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAATCCATCACGATTTATATTTATAATAAAAAATAATATTTTAAATACCATTAACAATGCATCATTTATTGAAAATGGAAATTTCAAGAAAAAATTTCGGGTTTATGCACGCAAGCAACATTTAGCAGCTTGA", "aa": "MKNPSRFIFIIKNNILNTINNASFIENGNFKKKFRVYARKQHLAA", "aa_hexdigest": "772b7f820bc7300daff0f5508763fe41", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5311.176100000002, "isoelectric_point": 11.202945137023924 }, "id": "NKIECMJPJD_894", "locus": "GCA000828735_00881" }, { "type": "cds", "sequence": "contig_1", "start": 517187, "stop": 517453, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGACAGTTTTAACAGCAATTGTAACAATTGCAACTGTTAATATTACAAGTCCCATGATTCTATTAGGTTTAATTATTCCAAATATAGTTAGGGCAACATTTAATAAACATGAACCATTATTTGTATTTTTTGTATCTATTGTATTTTCATTAGCATTGTTAGAAGTTTCATTATTTTTATCACTAAATTATCGTTTTGGTCCTAACTTTTTAATGGCTATTGTTAGTGCATTTGTGTTAATTTTTATTATGCGAAAACACGGATAA", "aa": "MTVLTAIVTIATVNITSPMILLGLIIPNIVRATFNKHEPLFVFFVSIVFSLALLEVSLFLSLNYRFGPNFLMAIVSAFVLIFIMRKHG", "aa_hexdigest": "78af84c2b2b4e56b0210ef0e94320ad2", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9827.89349999999, "isoelectric_point": 10.266477394104005 }, "id": "NKIECMJPJD_895", "locus": "GCA000828735_00882" }, { "type": "cds", "sequence": "contig_1", "start": 517457, "stop": 517576, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCTTTAATTTTGATTTGTTTATTTGCTTTTTTAGTATTATCACCAAAATTAAAAATTATGCAACGCGATTTTGCTTTGGCAAAATCTTTAGGAATCAAAGTTAATTTAATTTTTTGA", "aa": "MPLILICLFAFLVLSPKLKIMQRDFALAKSLGIKVNLIF", "aa_hexdigest": "d28722996d0aa30185c04702bbae5206", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4419.577899999999, "isoelectric_point": 10.032650566101072 }, "id": "NKIECMJPJD_896", "locus": "GCA000828735_00883" }, { "type": "cds", "sequence": "contig_1", "start": 517817, "stop": 517993, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTATTGGTATCCACCTATCGTTAAATTATTTGTAGCAATTTCTATGCCGGTTGCTGGATATGCCATTCAGATTACAACTCAGAATCGTTTATCATCACCTTCAACATTAGGATACATTCCAGTTTCAATTTTGGCTTATGTAGCAATGTTGATGATTGATCAAGGTAAAAGCTGA", "aa": "MYWYPPIVKLFVAISMPVAGYAIQITTQNRLSSPSTLGYIPVSILAYVAMLMIDQGKS", "aa_hexdigest": "ec76729dc423d6d0f51f4942d5c3ac61", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6409.598, "isoelectric_point": 9.22402362823486 }, "id": "NKIECMJPJD_897", "locus": "GCA000828735_00884" }, { "type": "cds", "sequence": "contig_1", "start": 518027, "stop": 518257, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAATAAAACAAGTAAATATTAATAAAAAAGATCTTTCTATTTATAAAAAACTCATTGAAGCAAAAAAATTTGTTAGTACACAAATTCGTCGTCCTTATATGATAACGATTATTGTATTAACATTTATTATCTTAGCTGTTGGTATGTTTTTTATGAATGTTTTTTATGAAAAATCTACATATTTTGATGCGAATTATAATACTCATGTAACCTATTTTCGAACTTGA", "aa": "MQIKQVNINKKDLSIYKKLIEAKKFVSTQIRRPYMITIIVLTFIILAVGMFFMNVFYEKSTYFDANYNTHVTYFRT", "aa_hexdigest": "2a4217d5cfa7c76ef4aacd9d4584236d", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9141.78509999999, "isoelectric_point": 9.872446632385255 }, "id": "NKIECMJPJD_898", "locus": "GCA000828735_00885" }, { "type": "cds", "sequence": "contig_1", "start": 518395, "stop": 518640, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTGAAGGCTTTGAACCAGGCACTGCTAAAGGTGAAGAAGTTTTAAAAAAATTTGAAACTAATTTAGCTAATTATGTTAATGGTTTAACAAAAGAAACAAAAAATCAATTTGTGCCAACAAAAGTTTTAAAAGAAAAACCAGTCGTGGGTAAGAATTTCTTTATTGAACGTAAAGGTAAGTTCTATGATGCATGTTATGGTTTTGCTGGTCAAGATTTAGTAGTTAATACATTAAATAAATGA", "aa": "MIEGFEPGTAKGEEVLKKFETNLANYVNGLTKETKNQFVPTKVLKEKPVVGKNFFIERKGKFYDACYGFAGQDLVVNTLNK", "aa_hexdigest": "bb77a0b9015a3894e2584ba47929564d", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9121.406899999996, "isoelectric_point": 9.040804481506349 }, "id": "NKIECMJPJD_899", "locus": "GCA000828735_00886" }, { "type": "cds", "sequence": "contig_1", "start": 518761, "stop": 518985, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATGTCTAACCCTCAAGATTATGCAGGTTTCCTTATTAATCCAGAAATTAAAACTGATGTGGATAAATTTAATGTAGGTAACGTATTCATCCCTCAACCAGTTTATTTCCCAGGAATTTACGCTTCGCAAGATGACCCATTTACTCCTGGCCTAGGTGCAAAATTTCCGCTTCCATTAAAAAATATTAATCAGATTCAAAGTTATGTTGATGATTATGGTTGA", "aa": "MMSNPQDYAGFLINPEIKTDVDKFNVGNVFIPQPVYFPGIYASQDDPFTPGLGAKFPLPLKNINQIQSYVDDYG", "aa_hexdigest": "ad9546d89d01679ed69091710c77eff3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8272.241700000002, "isoelectric_point": 4.142675971984862 }, "id": "NKIECMJPJD_900", "locus": "GCA000828735_00887" }, { "type": "cds", "sequence": "contig_1", "start": 519007, "stop": 519510, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCCTGAATATTTACGATCATATTATAATGAGAATTTAACAAAAGGTGATAGTTTTAAAAATGCGACTGCTGATAAATTTGCAAATTATAATATTGGTGTTTTATTAGTTGCAGATTCAAAAGTTGCAAGTCATGGTAAACTTTTAGAAGCAAGTGGCGGATCAGTTGGTGGATTATTAATTCAATCACGATCAACAGGTTATACTTCAAAAGCTCCGGCTGATATTGAACCAAATTTTTTATTTGGTAATCAAAATCCATATGTAGCAGAAAAAGAATCAGATCATGCTGGACGACCAAATTATTTTGTTGATCCTTTTAGTGGTTTAATTACAACAGGTAAAACATTGGATCGAATTTATGATGCTAAAAAATTTGACAATGTTCATAATGCTACAATTAATGGTAAAACTGGTTTTAACTCTTTTGAAGAATATGCACAAGTTGCATCTGATATAGCTCGTAAAAACTTTTCAAATTGGGCAAAATCTAATAATGAATGA", "aa": "MPEYLRSYYNENLTKGDSFKNATADKFANYNIGVLLVADSKVASHGKLLEASGGSVGGLLIQSRSTGYTSKAPADIEPNFLFGNQNPYVAEKESDHAGRPNYFVDPFSGLITTGKTLDRIYDAKKFDNVHNATINGKTGFNSFEEYAQVASDIARKNFSNWAKSNNE", "aa_hexdigest": "cb72d29e4ce8aee4a7493ffa1286c758", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18335.97129999999, "isoelectric_point": 6.303921699523926 }, "id": "NKIECMJPJD_901", "locus": "GCA000828735_00888" }, { "type": "cds", "sequence": "contig_1", "start": 519718, "stop": 519897, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATTAAAATATAAAAAAATATTATTAGGTGGATTAGTTGCTGCTATTAGTTTAGCATCAGCTACTGCAATAATTACCTCTTGTAAAGATACTAAAAAAAATAATTCAAATAATCCAACTACTAAATTTGATTTACAATCACGCCGTAAAGATTATGTTGAAGGTTTAAAGTTTTGA", "aa": "MKLKYKKILLGGLVAAISLASATAIITSCKDTKKNNSNNPTTKFDLQSRRKDYVEGLKF", "aa_hexdigest": "5f5736c2e83882e553a6402494869bed", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6533.618600000002, "isoelectric_point": 10.0100866317749 }, "id": "NKIECMJPJD_902", "locus": "GCA000828735_00889" }, { "type": "cds", "sequence": "contig_1", "start": 519987, "stop": 520280, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTTTAATCACTTCAGTATTAACAGGTGTTTCATATATTATGTCAGGGAATGTTTTATTTTTAGGTGTTGCATCCGCTAATATCGCTTATTCATTCCATGGTAAAAAAGTTATGCGTTCAATGCTTTTTTCAGGACTAATTACAACTTTATTTTTATTAATTGGGCAATTTTTCTTAAGCAATATTATTTTTAATTTGACACAACTATATTTTGAAACACCGATGATTACAACATTATTAGTAACACCAATCTTTATTGCCGTTATCTTATTAAAAAAACAAAAAGTTTAA", "aa": "MILITSVLTGVSYIMSGNVLFLGVASANIAYSFHGKKVMRSMLFSGLITTLFLLIGQFFLSNIIFNLTQLYFETPMITTLLVTPIFIAVILLKKQKV", "aa_hexdigest": "27ff01fbce6bb75db8f5e22d891e6177", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10781.041699999983, "isoelectric_point": 9.998095512390137 }, "id": "NKIECMJPJD_903", "locus": "GCA000828735_00890" }, { "type": "cds", "sequence": "contig_1", "start": 520374, "stop": 520811, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCCATAGGTATTTCTGCGTTTTTCTTACAAAGAATGACTAATAATCGTCTTGTTGATACATCTACTATTGGAATTGGTAATATTTGCTTAATTGGTCTAATAATCTTAGTATTATCATTAAGTTTTGAAAGTGGTTTTGAAGCACAGAATTTATTTCAAAAAATTGTGCCATTCGTTTTTATAATTTCTTCGATTATAGCTGGTTTATGCTTGTACTTTTTTTCAAAACGACGTCATGGTTTTAATAACCGTAAATTAATTATTTGTGGTGTTTTCATCAATTGTATATCAATTGCTTTGGCGATTAGTTTAAAAAGTAATTTAACAAAAGTAGGAAGTGAATATTTAGATTCACGAATTGTTGGGAGTTTTGATACAAGAAGCTATTTAGAAGAGTATTTAAGTTACGCAATATTTTTATTCATTATTTTATGA", "aa": "MSIGISAFFLQRMTNNRLVDTSTIGIGNICLIGLIILVLSLSFESGFEAQNLFQKIVPFVFIISSIIAGLCLYFFSKRRHGFNNRKLIICGVFINCISIALAISLKSNLTKVGSEYLDSRIVGSFDTRSYLEEYLSYAIFLFIIL", "aa_hexdigest": "cf96a610875687ddb26b092cc29f9a67", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16255.084799999986, "isoelectric_point": 9.022173118591308 }, "id": "NKIECMJPJD_904", "locus": "GCA000828735_00891" }, { "type": "cds", "sequence": "contig_1", "start": 521165, "stop": 521269, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACGGGGAAAATGCTAGACCTATTGAATTAAATATTCCCAAATTTAATAAAGATAATGTTCAACATATTCGTGCTTTTAAAGATGATTTTAAAAACAAATAA", "aa": "MNGENARPIELNIPKFNKDNVQHIRAFKDDFKNK", "aa_hexdigest": "0b61304d82108d39faba61349fb7bfc2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4042.5388000000003, "isoelectric_point": 9.397636985778806 }, "id": "NKIECMJPJD_905", "locus": "GCA000828735_00892" }, { "type": "cds", "sequence": "contig_1", "start": 521270, "stop": 521857, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTATGCCTAACTTTACTCCAACAGCTAAAATGGTAGATGTTAATTTTAAACCAGAAGACTTAGAATTCTTGAGTAATGTTTGCATTAATGAACCTATGTATTGTCCAACAATATATAGTGATCCAAATGATAAATATTTACCTGGATTAGGTGCAAAATTCCCTATTCCAGTAAATCATATTTCACAAGTGCAAAATTATATTGATGATTATGGACAAATTGGTGGAAAAGTATTAAATGGTACTGGTAATCCAGAAGCATCAAAACAAGTTGGCCCAACTTTAGGTGATGCTTTTAAAGGTACAAGTGACGTTGTAGTTTTCTGTTATAACGAATACGGTTTATTAAATTACAAACCCGGAACTGAAGAAGGCGAAGCTTTAGTACAACAATTTGAAAAGAATTTAACAAATTATGTTAATGGTTTAGATATTAACACAAAAAATAATTTTAATCCTACAAAAATGTTAAAAGAAAAACCGGTTGTTGGTAAAAATTTCTTTATTATTCGTAAAAGTTCGTTCTATGATGCATTTTTAGGTTTTTTAGGAACAATTCACAATTTTAATACATTTAATAAATGA", "aa": "MVMPNFTPTAKMVDVNFKPEDLEFLSNVCINEPMYCPTIYSDPNDKYLPGLGAKFPIPVNHISQVQNYIDDYGQIGGKVLNGTGNPEASKQVGPTLGDAFKGTSDVVVFCYNEYGLLNYKPGTEEGEALVQQFEKNLTNYVNGLDINTKNNFNPTKMLKEKPVVGKNFFIIRKSSFYDAFLGFLGTIHNFNTFNK", "aa_hexdigest": "324809a795e8ba97323c2a162dc2294a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 21806.51810000002, "isoelectric_point": 5.402057456970215 }, "id": "NKIECMJPJD_906", "locus": "GCA000828735_00893" }, { "type": "cds", "sequence": "contig_1", "start": 521897, "stop": 522202, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAATCACGAGTTTCTGGTTTGCCATCACACTATCCTGCTGTTCTAACACCAAAATTTTTATTTGATCCTAATTTAAATCCAAATTTAAAAAAATATGCTTCTGCTAATTATGCGTTACCTAATTATTACTCTGACCCACTAGATGGGATTATTTTAACAGGAAAAACTTTAGATCGTATTTATGATGCTAAAAAGTTTTCTAATGTTTATAATGCACATGTAGCTGGCAAAACACATTTTGAGACATTCACAGATTTTGCAAAGGCAATGGTTGATGAAACGCGTCGTGGAATTTCACAATGA", "aa": "MQSRVSGLPSHYPAVLTPKFLFDPNLNPNLKKYASANYALPNYYSDPLDGIILTGKTLDRIYDAKKFSNVYNAHVAGKTHFETFTDFAKAMVDETRRGISQ", "aa_hexdigest": "0f58190332ed439183843845d1fdf313", "start_type": "ATG", "rbs_motif": "GGxGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11374.75419999999, "isoelectric_point": 9.18766345977783 }, "id": "NKIECMJPJD_907", "locus": "GCA000828735_00894" }, { "type": "cds", "sequence": "contig_1", "start": 522608, "stop": 522814, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGATGAAAGCTAAGAAATTTTTAATTACAGGATTATTAGGTGTAGTTAGTTTAGCAACTATTACCACAGTAGCTACAGCTTGTACAAATGGCAATAATCAAAAGGCTAATACAGTAAATAATAAGTCTGATTTAAAAACAAATCCAAATATTAATTTAACTCAACAAGATGTAGAAAATTTGGAAAAACAAAGACAACAATGA", "aa": "MKMKAKKFLITGLLGVVSLATITTVATACTNGNNQKANTVNNKSDLKTNPNINLTQQDVENLEKQRQQ", "aa_hexdigest": "1e4a30e5ac40e86ac7c58d6aeb731d31", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7445.471400000003, "isoelectric_point": 9.807978248596193 }, "id": "NKIECMJPJD_908", "locus": "GCA000828735_00895" }, { "type": "cds", "sequence": "contig_1", "start": 522957, "stop": 523832, "strand": "-", "frame": 1, "gene": "ribF", "product": "riboflavin biosynthesis protein RibF", "db_xrefs": [ "EC:2.7.1.26", "EC:2.7.7.2", "GO:0003919", "GO:0005737", "GO:0006747", "GO:0009398", "KEGG:K11753", "SO:0001217", "UniRef:UniRef50_Q9PQD6", "UniRef:UniRef90_Q9PQD6" ], "nt": "ATGCAATTGAAATTGAACAAGTCTATGATTATTGAAATAACATCAACAAATATTTATAAAATTAAGCAACAATTCGTCATTAATGAATTAATTATTGGTTTTTTTGATGGAATTCATTTAGGTCATATGAATTTATTATCAGATCCTAATAACCAAACTATTTTAACTTTTAAAAATATCCCACGTAAAATAAAAAAATTATATGATTTTAATGAGCGAATTCAACAATTAGAAGATTTAGGATTTAAAAGGATTTTTATTTATGATATTGATCAAAACAATTTAAGTGGGGAAGAATTTATTGATCAAATTTTAAAGCCACTAACTCCAAAAAAAATTATTGTTGGTGCTAATTTTACTTATGGCAATAATTTTTGTAATGCATCTAGTCTAAAACAATATTTTAATGTTGAAATTAAAATTATTACTAATGATGTTAGTACAACTAAAATTAAAGAATTAATTATAAATAAACAGGTTGAAATAGCTAATAAATTATTAATTAAACCATATTATCGTGTGGGAAATGTTGTACGTGGCGATCAAATAGCTCGTAATATTGGTTTTAATACTGCTAATATTTTATGTGATAATAATTTAATTGATATTGCTGAAGGTGTTTACAAAGCACAAGTAATTTTTAATAATAAAAAGTATGATAGTGTAGTTTATTTAGGAATCCCTAAAACAATTAATACTCGCTCTTTTTCAATGATTGAAGCACATATTTTAGACTTTAATCAAAATATTTATGACGAAAGAATTAAGATTGTTTTTTTGAAATATTTAGCTCCTAATTTAAAATTTAATAATATAGATGAATTAATCACTGCAATTAAAAATTATATTAAATTGGTTCTTGATAAAACAAATTAG", "aa": "MQLKLNKSMIIEITSTNIYKIKQQFVINELIIGFFDGIHLGHMNLLSDPNNQTILTFKNIPRKIKKLYDFNERIQQLEDLGFKRIFIYDIDQNNLSGEEFIDQILKPLTPKKIIVGANFTYGNNFCNASSLKQYFNVEIKIITNDVSTTKIKELIINKQVEIANKLLIKPYYRVGNVVRGDQIARNIGFNTANILCDNNLIDIAEGVYKAQVIFNNKKYDSVVYLGIPKTINTRSFSMIEAHILDFNQNIYDERIKIVFLKYLAPNLKFNNIDELITAIKNYIKLVLDKTN", "aa_hexdigest": "42f3b7dd4054e4665ae0ad4d9d6dba47", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_Q9PQD6", "query_cov": 0.9725085910652921, "subject_cov": 1.0, "identity": 0.975, "score": 545.0, "evalue": 1.37e-195, "valid": true, "gene": "ribF", "product": "riboflavin biosynthesis protein RibF", "ec_ids": [ "2.7.1.26", "2.7.7.2" ], "uniref50_id": "UniRef50_Q9PQD6", "kegg_orthology_id": "K11753", "go_ids": [ "GO:0003919", "GO:0005737", "GO:0006747", "GO:0009398" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQD6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQD6" ], "product": "Riboflavin biosynthesis protein" }, "genes": [ "ribF" ], "id": "NKIECMJPJD_909", "locus": "GCA000828735_00896" }, { "type": "cds", "sequence": "contig_1", "start": 523798, "stop": 523992, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATTTCAATTATGAAAAACAAATTTTAAAAATTTCTTTACATGTTTCTAAAGGTTTTTATGTTCGTTCTTTTGCTGTTGATTTAGCTACTAAAATTAATAATCTAGCTCATTTATTGACATTAATAAGGACGCAAAGTGGACCTTTTGAGATTAAAGATGCAATTGAAATTGAACAAGTCTATGATTATTGA", "aa": "MDFNYEKQILKISLHVSKGFYVRSFAVDLATKINNLAHLLTLIRTQSGPFEIKDAIEIEQVYDY", "aa_hexdigest": "0eea71d54a267a4df4ca5b9686e6f0dc", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7449.492499999998, "isoelectric_point": 6.025240135192871 }, "id": "NKIECMJPJD_910", "locus": "GCA000828735_00897" }, { "type": "cds", "sequence": "contig_1", "start": 524113, "stop": 524307, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGGAATTAATGAAGGAACAAAACTATTAACAAAATTAATACTAGATAATAAATCATATATTGCTGAAATTAAATTTGGAACATCAACAAATACTTATGATGCAGCAGGTGAAATTGTTTCATCTACAAACCGAATGGTTACATTAACTGAGGTTACTAAAATAGTTAAAGATTTTTATAAAAATGATTATTGA", "aa": "MGINEGTKLLTKLILDNKSYIAEIKFGTSTNTYDAAGEIVSSTNRMVTLTEVTKIVKDFYKNDY", "aa_hexdigest": "60512212ac0e2850146302164323f9ed", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7169.098900000004, "isoelectric_point": 6.124367332458495 }, "id": "NKIECMJPJD_911", "locus": "GCA000828735_00898" }, { "type": "cds", "sequence": "contig_1", "start": 524563, "stop": 524739, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAACAATTTTAATTGTAAATAAATCATTTCGTAAAAAGCGCAGTTTTGACACTCGTTTTTTGCATGATAGTGGAACGATTTATAGTAATGATCGTTTTTTAAAAATTGTGCGTGAAAGCTTTGATGCTCATATTAAGAAAAAAAGTAAAATTCTATTTTATTTCATGGAATGA", "aa": "MKTILIVNKSFRKKRSFDTRFLHDSGTIYSNDRFLKIVRESFDAHIKKKSKILFYFME", "aa_hexdigest": "aee2a06cb70b68d9d43acd9e35eeac21", "start_type": "TTG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7076.253599999998, "isoelectric_point": 10.229021263122558 }, "id": "NKIECMJPJD_912", "locus": "GCA000828735_00899" }, { "type": "cds", "sequence": "contig_1", "start": 525139, "stop": 525588, "strand": "+", "frame": 1, "gene": null, "product": "Unique hypothetical membrane lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQD8", "UniRef:UniRef90_Q9PQD8" ], "nt": "ATGATTAAAAAAATTAGACCTAGAACTAATAAAATGAGCTTAGAAGATTATTTGATCGCAAGGATGAATTTAATTAATTCATTCAAATTTATACTAAAACGTAAAGTATTATTTATTGAAGGGATCAAAGAATATAACCATCGTTTTGTTTTTAATATGAATGCTGATTATAATTTAATGAGAATAATGAATTATATTTATTCTGGTTTGGCAGAATTAAACATCATTATGGTTATTGATATGGATAGTGATGAAAAAATTTTTGAATTACAAAAAGTAATTAAACAAGATTTTGATAATTTAGATTTAATCATTATAGAAAAAGAAATAGCAGAGTCACTAAAATCAAAATACCAAAATGGTTGTTTAAATAAAAAAACTAATTTAATTAACATTAATAATAAAGAAATTGATCAAAGTCAACAAACTCAAGATATTAACATTTACTAA", "aa": "MIKKIRPRTNKMSLEDYLIARMNLINSFKFILKRKVLFIEGIKEYNHRFVFNMNADYNLMRIMNYIYSGLAELNIIMVIDMDSDEKIFELQKVIKQDFDNLDLIIIEKEIAESLKSKYQNGCLNKKTNLININNKEIDQSQQTQDINIY", "aa_hexdigest": "33fdc0ccb4f2f782b7db014cca8506d6", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 524839, "stop": 525588, "observations": { "mutation": [ 525031, 525052 ], "stop": [ 525031, 525052 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQD8", "query_cov": 1.0, "subject_cov": 0.436950146627566, "identity": 1.0, "score": 284.0, "evalue": 5.11e-94, "gene_start": 1, "gene_end": 149, "reference_start": 193, "reference_end": 341, "reference_sequence": "MKTILIVNKSFRKKRSFDTRFLHDSGTIYSNDRFLKIVRESFDAHIKKKSKILFYFMEWWFQTTNYKLNPEISHAKRSLRSWLIEIFFLFCIVALIVCILAGVARPIIDCVIKSITLSRGQFKGDLIIDGQIIMKNLSPSNINGLTGGSSILNVDQWELIPKVWKAYFSSPSVVVLILLVLFFLFFSCLYIYMIKKIRPRTNKMSLEDYLIARMNLINSFKFILKRKVLFIEGIKEYNHRFVFNMNADYNLMRIMNYIYSGLAELNIIMVIDMDSDEKIFELQKVIKQDFDNLDLIIIEKEIAESLKSKYQNGCLNKKTNLININNKEIDQSQQTQDINIY" }, "paralog": false, "identity": 0.9919678714859438, "score": 466.0, "evalue": 2.33e-168, "description": "Internal stop codon at 525031, 525052. Nonsense mutation around 525031, 525052.", "psc": { "uniref90_id": "UniRef90_Q9PQD8", "product": "Unique hypothetical membrane lipoprotein", "uniref50_id": "UniRef50_Q9PQD8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQD8", "UniRef:UniRef50_Q9PQD8" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQD8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQD8" ], "product": "Unique hypothetical membrane lipoprotein" } }, "id": "NKIECMJPJD_913", "locus": "GCA000828735_00900" }, { "type": "cds", "sequence": "contig_1", "start": 525815, "stop": 526144, "strand": "+", "frame": 2, "gene": null, "product": "Sigma-70 family RNA polymerase sigma factor", "db_xrefs": [ "RefSeq:WP_006688721.1", "SO:0001217", "UniParc:UPI00000C1C30", "UniRef:UniRef100_A0AAC9T0X2", "UniRef:UniRef50_A0AAC9T0X2", "UniRef:UniRef90_A0AAC9T0X2" ], "nt": "ATGTGTATTAGTAAAATAGATATGGCTAAGGTAAAAAAATTTTTTAAAGAATATTTATTTGCTAAATTTCAATGCAAAAATCGAGAACTATATCGTCAATTAAAAGATTATGATCCAAAAGATGATCAAAAATATTTAAAATGGGAGCATTTTGTTGAATATGTTGAGCAAGTTCTAGAAGCATTAGATAAAACTAGTGCTCAAATTATTAAAGAAATTTATATTCAAAATAAAAGAATTTGTGAATTACCTTATAGTTATTCAACATATTATGCATATCGTAAAAAAGCAATTATTGAATTACTTGCATATCTTGATTTAAAAATTTAA", "aa": "MCISKIDMAKVKKFFKEYLFAKFQCKNRELYRQLKDYDPKDDQKYLKWEHFVEYVEQVLEALDKTSAQIIKEIYIQNKRICELPYSYSTYYAYRKKAIIELLAYLDLKI", "aa_hexdigest": "2efdadbda6483303041e757dd1861977", "start_type": "ATG", "rbs_motif": "GGAGG", "ups": { "uniparc_id": "UPI00000C1C30", "ncbi_nrp_id": "WP_006688721.1", "uniref100_id": "UniRef100_A0AAC9T0X2", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1C30", "RefSeq:WP_006688721.1", "UniRef:UniRef100_A0AAC9T0X2" ] }, "ips": { "uniref100_id": "UniRef100_A0AAC9T0X2", "uniref90_id": "UniRef90_A0AAC9T0X2" }, "psc": { "uniref90_id": "UniRef90_A0AAC9T0X2", "product": "Sigma-70 family RNA polymerase sigma factor", "uniref50_id": "UniRef50_A0AAC9T0X2" }, "pscc": { "uniref50_id": "UniRef50_A0AAC9T0X2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T0X2" ], "product": "Sigma-70 family RNA polymerase sigma factor" }, "genes": [], "id": "NKIECMJPJD_914", "locus": "GCA000828735_00901" }, { "type": "cds", "sequence": "contig_1", "start": 526174, "stop": 526293, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACCATTTGTCTGAATCAATTTTTATAGATGTAATTTATGATGAATTAACAAAATTTTATGGAAATGATGCAAATTTAAATAAATCTTTATCAATTATAAAAATTAACTATATTTAA", "aa": "MNHLSESIFIDVIYDELTKFYGNDANLNKSLSIIKINYI", "aa_hexdigest": "021b7b22d06eb76dfdab67e4e300a2b8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4550.1448, "isoelectric_point": 4.8263353347778315 }, "id": "NKIECMJPJD_915", "locus": "GCA000828735_00902" }, { "type": "cds", "sequence": "contig_1", "start": 526330, "stop": 526611, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTAAACGTTTAGGCTTATTGAATCTTAAGCAAGATGAACAGAATCCATATCTTGTTGTAGGAGAATTAAAATTAGGTGTTAGTGTTGATTACATTAGTCGGATTATTAAACAAAAATTTTTATCACCTATTATTAAATATAACAACGTTTTTAGACTAGAAACAAATCTTAAAAAAATTGGTATTATAGGAGGTAGTGGTTATAAATTTGCTGATGACGCATTTAATCGTTATCAACTAGATATGTTAATTACAAGTGATTTAAAATATCATAATTGA", "aa": "MAKRLGLLNLKQDEQNPYLVVGELKLGVSVDYISRIIKQKFLSPIIKYNNVFRLETNLKKIGIIGGSGYKFADDAFNRYQLDMLITSDLKYHN", "aa_hexdigest": "a4c99b7f7ba938b5def71fa329dff4c8", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10710.387899999987, "isoelectric_point": 9.521416282653806 }, "id": "NKIECMJPJD_916", "locus": "GCA000828735_00903" }, { "type": "cds", "sequence": "contig_1", "start": 526943, "stop": 527608, "strand": "-", "frame": 2, "gene": "trmK", "product": "SAM-dependent methyltransferase", "db_xrefs": [ "COG:COG2384", "COG:J", "EC:2.1.1.217", "KEGG:K06967", "RefSeq:WP_006688536.1", "SO:0001217", "UniParc:UPI00000C1C2F", "UniRef:UniRef100_A0A2C9DZ44", "UniRef:UniRef50_A0A2C9DZ44", "UniRef:UniRef90_A0A2C9DZ44" ], "nt": "GTGAATCCGAAAACTAGAATTGCTTTTATTGCTGATTTAATTAATGACGCTCAAAATGTTGTCGATGTTGGTAGTGATCACGCATATTTAGCTAAAATCTTATTGGTTAACAATAAAGCACAACATGTTACTAATATAGAAGTTAATCAAGGTCCATTAGAAAACGGAATCAATAATTTGACTAAAAATAATCTTTTAAAACGAACTACTAATATTTTGAATGATGGTTTCAAGGGATTAATTTTGGAACAAACATTTGATTATTGTGTTATTGCAGGGATGGGAGCAACAAGTATAATAGAAATTTTAGAACAGAATAAAAATCAAATTAAAAATTATATTTTACAACCAAATACTCAATCATATAAAATACGTAAATATCTAAATAATCACTCATATCAAATTAAAGTTGAACACATTTTTGTTGAAAAAAAAATTTTTTATGAAATAATCATTTGTTCTAAAACTATTAATGTACCAAAATTAATAGAAAAAGATTATTATATAAGTAGCCAGATGCATAAGGATTCATTGAATTTATATTTTAAATTTTTAAAGAGACGTTATGAATACTTAAAAGCTTTGGATATTTCGCTAGTTAGTGAAAATATAGTTAAAGAATATGAATATATAAAGGAATTTATTAATGAAAAAAATGGATATTAA", "aa": "MNPKTRIAFIADLINDAQNVVDVGSDHAYLAKILLVNNKAQHVTNIEVNQGPLENGINNLTKNNLLKRTTNILNDGFKGLILEQTFDYCVIAGMGATSIIEILEQNKNQIKNYILQPNTQSYKIRKYLNNHSYQIKVEHIFVEKKIFYEIIICSKTINVPKLIEKDYYISSQMHKDSLNLYFKFLKRRYEYLKALDISLVSENIVKEYEYIKEFINEKNGY", "aa_hexdigest": "921fe2f18e6d404483d559f1d2274f36", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00000C1C2F", "ncbi_nrp_id": "WP_006688536.1", "uniref100_id": "UniRef100_A0A2C9DZ44", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1C2F", "RefSeq:WP_006688536.1", "UniRef:UniRef100_A0A2C9DZ44" ] }, "ips": { "uniref100_id": "UniRef100_A0A2C9DZ44", "uniref90_id": "UniRef90_A0A2C9DZ44" }, "psc": { "uniref90_id": "UniRef90_A0A2C9DZ44", "gene": "TrmK", "product": "SAM-dependent methyltransferase", "ec_ids": [ "2.1.1.217" ], "uniref50_id": "UniRef50_A0A2C9DZ44", "cog_id": "COG2384", "cog_category": "J", "kegg_orthology_id": "K06967" }, "pscc": { "uniref50_id": "UniRef50_A0A2C9DZ44", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2C9DZ44" ], "product": "SAM-dependent methyltransferase" }, "genes": [ "trmK" ], "id": "NKIECMJPJD_917", "locus": "GCA000828735_00904" }, { "type": "cds", "sequence": "contig_1", "start": 527592, "stop": 528119, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF04545.22" ], "nt": "ATGGTTGAAACAATTAACAAATTAACTAAAGCCGAGCGAAATTTAATTCAAGAATTAGGAAGGGATCCAACGGCTGAAGAAATTGCGCAAGCTATGAACAAAGTTTCACAAGCAAAAAATCAAAAAGAACAGTTAATCACTGCACAAAAAGTTGTTGAGATTAAAAAATTAAATGTTGACCCAGTTTCATTAGATAAACAAATTGGTCATGATGAAGAATCACAATTTTCAGATTTCATTTCTGATGATGAAATTATTTCTCCAGAAAAATATACTGAAAAAAAAGCATTAAATGATCAGATTAATGAAATGTTTGAAAAAGTTTTAAACGATAATGAACAACGTGTTATTAAAATGCGGTATGGTTTATTACCCTTCGAACGTCCTTATACACTTGAAGAAGTTGGTGAATATTTAGGAGTTACACGCGAACGTGCACGTCAAATTGAATCAAAAGCAATTCGTAAATTAAAACATCCATCAAAAACCGCAAAACTACGTTCATTTATTGGTGAATCCGAAAACTAG", "aa": "MVETINKLTKAERNLIQELGRDPTAEEIAQAMNKVSQAKNQKEQLITAQKVVEIKKLNVDPVSLDKQIGHDEESQFSDFISDDEIISPEKYTEKKALNDQINEMFEKVLNDNEQRVIKMRYGLLPFERPYTLEEVGEYLGVTRERARQIESKAIRKLKHPSKTAKLRSFIGESEN", "aa_hexdigest": "cccd2081e377d6d921486d29701a108d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF04545.22", "name": "Sigma70_r4", "length": 52, "aa_cov": 0.29714285714285715, "hmm_cov": 0.9038461538461539, "evalue": 3.764474066225992e-21, "score": 69.9921875, "start": 108, "stop": 159 } ], "seq_stats": { "molecular_weight": 20233.731200000017, "isoelectric_point": 5.631061744689941 }, "id": "NKIECMJPJD_918", "locus": "GCA000828735_00905" }, { "type": "cds", "sequence": "contig_1", "start": 528186, "stop": 528938, "strand": "-", "frame": 1, "gene": "rpoD", "product": "RNA polymerase sigma factor", "db_xrefs": [ "COG:COG0568", "COG:K", "SO:0001217", "UniRef:UniRef50_Q9PQE2", "UniRef:UniRef90_Q9PQE2" ], "nt": "ATGTCAACAAAACAAAATGAACCATTATTTGTAAATCTAGAGGATTTAAAACAAAAAGTTAAATTATCATTTACTGAAGAATTCTCATTTTCGTATGCACTTTCTGAACTTGAAGCAGGAATTTTTGAAAGTCGTAATTTAAAAGGTGCAGATGCTGGTGATCCTGAAGAAATTTTATTAAATGTGGTTTTAGATGTTAGTAAGAAAAAACGTTCTCGAAATGAAATTAAATTTAATAAACTTCAAAATTATTTTATTCATATGAATTTACGTGAAGAACACTTTAGTGAAATTATTGATGTTCTAGAAGCTATTGGAATTCGTGTGCCTGATTATGATTTGGTGATGCAATCAAAATCAAAGACAAACACCAAAAAAAAGGATGAATATGGTATTGATGATACTTTAGAAATTAGCACATCAAAAATTGGTTTTTCATCAACAACAACCGAAAAAGTTGATGATGGTATTAAAGCTTATTTAGGAGTACTTGGAGAATCAAAAATGTTACGCTCTGATGAGGAAACTGAATATGCTAAAATGGTTATTAGTGACGATCCATCTCTAGTTAAAATTGGTAAAAATCAACTTTATACATCAAATATGCGTTTAGTTACTTCTATTGCTAAAAAATATTTAAATCGTGGATTAGACTTAGAAGATTTAATTCAAGAAGGGTCATCCGGTTTATTAAAAGCAATTGATAAATTTGATCATGAAAAAGGACATAAATTTAGTACATATGCAACATGA", "aa": "MSTKQNEPLFVNLEDLKQKVKLSFTEEFSFSYALSELEAGIFESRNLKGADAGDPEEILLNVVLDVSKKKRSRNEIKFNKLQNYFIHMNLREEHFSEIIDVLEAIGIRVPDYDLVMQSKSKTNTKKKDEYGIDDTLEISTSKIGFSSTTTEKVDDGIKAYLGVLGESKMLRSDEETEYAKMVISDDPSLVKIGKNQLYTSNMRLVTSIAKKYLNRGLDLEDLIQEGSSGLLKAIDKFDHEKGHKFSTYAT", "aa_hexdigest": "42b842bed76bb46208c05447051f0b43", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "genes": [ "rpoD" ], "truncated": "5-prime", "pseudogene": { "start": 527883, "stop": 528938, "observations": { "mutation": [ 528185, 528188 ], "stop": [ 528185, 528188 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQE2", "query_cov": 1.0, "subject_cov": 0.5580357142857143, "identity": 0.94, "score": 456.0, "evalue": 1.39e-158, "gene_start": 1, "gene_end": 250, "reference_start": 1, "reference_end": 250, "reference_sequence": "MSTKQNEPLFENLEDLKQKVKLSFTEEFSFSYALSEREAGIFESRNLKGADASNPEEILLNVVLDVSKRKRSRNEIKFNKLQNYFIHMNLRDEHFSEIVDVLENIGIRVPDYELVMQSKSKSTAKKKDEYGIDDTLEISTSKIGFSSTTTEKVDDGIKAYLGVLGESKMLRSDEETEYAKMVISNDPALIKIGKNQLYTSNMRLVTSIAKKYLNRGLDLEDLIQEGSSGLLKAIDKFDHEKGHKFSTYATWWIRQSITRAIADQARQIRIPVHMVETINKLTKAERSLIQELGRDPTAEEIAQAMNKASQAKNQKEQLITAQKVVEIKKLNVDPVSLDKQIGHDEESQFSDFISDDEIISPEKYTEKKALNDQINEMFEKVLNDNEQRVIKMRYGLLPFERPYTLEEVGEHLGVTRERARQIESKAIRKLKHPSKTAKLRSFIGESEN" }, "paralog": false, "identity": 0.9458689458689459, "score": 642.0, "evalue": 3.42e-234, "description": "Internal stop codon at 528185, 528188. Nonsense mutation around 528185, 528188.", "psc": { "uniref90_id": "UniRef90_Q9PQE2", "gene": "RpoD", "product": "RNA polymerase sigma factor", "uniref50_id": "UniRef50_Q9PQE2", "cog_id": "COG0568", "cog_category": "K", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQE2", "UniRef:UniRef50_Q9PQE2", "COG:COG0568", "COG:K" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQE2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQE2" ], "product": "RNA polymerase sigma factor SigA" } }, "id": "NKIECMJPJD_919", "locus": "GCA000828735_00906" }, { "type": "cds", "sequence": "contig_1", "start": 529024, "stop": 530433, "strand": "-", "frame": 3, "gene": "lon", "product": "endopeptidase La", "db_xrefs": [ "COG:COG0466", "COG:O", "EC:3.4.21.53", "GO:0004176", "GO:0004252", "GO:0005524", "GO:0005737", "GO:0006508", "GO:0006515", "GO:0016887", "GO:0034605", "GO:0043565", "SO:0001217", "UniRef:UniRef50_B1AIY7", "UniRef:UniRef90_B1AIY7" ], "nt": "ATGTCTGTAAGAACTCATTTAGATGATAATCACTATGGTATTGAAAAGGTAAAAGAAAGAATTTTAGAGTATTTAGCATTACGTATGCGTAACCCTAATCTTAAAGGTCCAATCATTTGTTTAGTTGGCCCTCCAGGTGTTGGTAAAACATCACTTGTTACTTCGATTGCACAAGCTTTAAATAAAAAATTTGTTAAAGTTAGTTTAGGTGGTGTGCGAGATGAATCTGAAATTCGAGGTCATCGTAAAACTTATGTTGGAGCTATGCCCGGACGTATTATTAAGGGTATGAAAAAGGCTGGCGTTATCAATCCTTTATTCCTATTAGATGAAATTGATAAAATGACATCTGATCAACGAGGAGATCCTGCTGCCGCAATGCTTGAAGTATTAGATCCTGAACAAAATAAAAATTTTTCAGATAATTACATTGAAGAAGAATATGATTTGAGCAAAGTGATGTTTATGGCAACAGCTAATTATTATCAACAAATTCCTTATGCTTTGATTGATCGTTTAGAAGTAATTGAACTATCGAGTTATACAGCAATTGAAAAACGTGAAATAGCAAAATCACATCTATTAAAAAGAATTTTTATGGATGCTAAATTAAATGAAAATGAACTTATTTTTACTGATGATGCTTTAGATTTTATTATTAATCATTATACTAAAGAAGCTGGAGTACGAGAATTAGATCGTCAATTAGGTCATATTGTACGCAAGTACATTGTTGAAACTTATAAGAATAAAAATAATCAATCACCTTTGAATTTAAAAGTTGATGAAGCCATGATTATTAAATATTTAGGTAAAATTAAGTTTGATTTTAATAAAAAAGAAGAGACAACAATTCCAGGAATTGTTAATGGAATGGCTTATACAGCGGCTGGTGGCGATTTATTGCCTATTGAAGTTAATCATTCAACCAATGGAAAAGGTGGAAATGTAACTATTACTGGAAATCTTGAAAAAACAATGAATGAATCTGTATCTGTTGCTTTAGGTTTTGTTAAAGCAAATGCAGATAAATATGGCATTGATACTAAAAAAGTTTCTTTTAAAGAAATTGATATTCATGTTCATGTTCCAAGCGGAGGAATTCCTAAAGATGGACCGTCTGCTGGAATTGCGATTACAACAGCAATTATTAGTTCATTAAGTCAAAGACCAGTGCGTACAACACTATCAATGACAGGAGAAATTATGCTACGTGGTAATGTTGGAATTATTGGTGGTGTTAAAGAAAAAGTTATTTCAGCATATCGTGCTGGAGTTCGTGAAATTATTTTACCAATTGATGACGAACGTTACTTAGAAGATGTTCCAAAGTATATATTAGATGATATTAAAATTCATTTGGTAAAACACTATGATGAAGTTTATAATATTGTTTTTGGAGAAAAATAA", "aa": "MSVRTHLDDNHYGIEKVKERILEYLALRMRNPNLKGPIICLVGPPGVGKTSLVTSIAQALNKKFVKVSLGGVRDESEIRGHRKTYVGAMPGRIIKGMKKAGVINPLFLLDEIDKMTSDQRGDPAAAMLEVLDPEQNKNFSDNYIEEEYDLSKVMFMATANYYQQIPYALIDRLEVIELSSYTAIEKREIAKSHLLKRIFMDAKLNENELIFTDDALDFIINHYTKEAGVRELDRQLGHIVRKYIVETYKNKNNQSPLNLKVDEAMIIKYLGKIKFDFNKKEETTIPGIVNGMAYTAAGGDLLPIEVNHSTNGKGGNVTITGNLEKTMNESVSVALGFVKANADKYGIDTKKVSFKEIDIHVHVPSGGIPKDGPSAGIAITTAIISSLSQRPVRTTLSMTGEIMLRGNVGIIGGVKEKVISAYRAGVREIILPIDDERYLEDVPKYILDDIKIHLVKHYDEVYNIVFGEK", "aa_hexdigest": "802b673f74080daba6e9994be1b061ce", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0025901479", "query_cov": 0.9701492537313433, "subject_cov": 0.8956692913385826, "identity": 0.5489999999999999, "score": 489.0, "evalue": 4.25e-167, "valid": false, "gene": "lon", "product": "endopeptidase La", "ec_ids": [ "3.4.21.53" ], "uniref50_id": "UniRef50_A0A7C6Y0Z4", "go_ids": [ "GO:0004252", "GO:0005524", "GO:0006508", "GO:0016887" ] }, "genes": [ "lon" ], "truncated": "3-prime", "pseudogene": { "start": 529024, "stop": 530733, "observations": { "mutation": [ 530466, 530484 ], "stop": [ 530466, 530484 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_B1AIY7", "query_cov": 1.0, "subject_cov": 0.5929203539823009, "identity": 1.0, "score": 918.0, "evalue": 0.0, "gene_start": 1, "gene_end": 469, "reference_start": 323, "reference_end": 791, "reference_sequence": "MKKPILISRAIVVLPYETTTIEVGRPKSIQAIDLAKQSSSKEIIIISQKDIDTDEVVNFDELYKVGTLVKIKSIIDNFDEGYSIEVEGLKAVYINNENDVIDAIEYEYEDVITNPILSNKDEIAINGINSEIFNIINKRSRHKNINFDNMHALISLEKEKFAYLAAATYINDYDNEISEKTIEDRINILLQPNLLLVHETILHLLFDQLVDKRVIEDEVEKTITDKINNNFQKQQREFYLREKLKVVKEQLGELSSREEDADKIRAKIEDLELPPNVKERALAELNRFENAMSSNESSVIKSYLDWLLDLPWTQQGVDNTDLMSVRTHLDDNHYGIEKVKERILEYLALRMRNPNLKGPIICLVGPPGVGKTSLVTSIAQALNKKFVKVSLGGVRDESEIRGHRKTYVGAMPGRIIKGMKKAGVINPLFLLDEIDKMTSDQRGDPAAAMLEVLDPEQNKNFSDNYIEEEYDLSKVMFMATANYYQQIPYALIDRLEVIELSSYTAIEKREIAKSHLLKRIFMDAKLNENELIFTDDALDFIINHYTKEAGVRELDRQLGHIVRKYIVETYKNKNNQSPLNLKVDEAMIIKYLGKIKFDFNKKEETTIPGIVNGMAYTAAGGDLLPIEVNHSTNGKGGNVTITGNLEKTMNESVSVALGFVKANADKYGIDTKKVSFKEIDIHVHVPSGGIPKDGPSAGIAITTAIISSLSQRPVRTTLSMTGEIMLRGNVGIIGGVKEKVISAYRAGVREIILPIDDERYLEDVPKYILDDIKIHLVKHYDEVYNIVFGEK" }, "paralog": false, "identity": 0.9964850615114236, "score": 1108.0, "evalue": 0.0, "description": "Internal stop codon at 530466, 530484. Nonsense mutation around 530466, 530484.", "psc": { "uniref90_id": "UniRef90_B1AIY7", "gene": "lon", "product": "endopeptidase La", "ec_ids": [ "3.4.21.53" ], "uniref50_id": "UniRef50_B1AIY7", "cog_id": "COG0466", "cog_category": "O", "go_ids": [ "GO:0004176", "GO:0004252", "GO:0005524", "GO:0005737", "GO:0006508", "GO:0006515", "GO:0016887", "GO:0034605", "GO:0043565" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_B1AIY7", "EC:3.4.21.53", "UniRef:UniRef50_B1AIY7", "COG:COG0466", "COG:O", "GO:0004176", "GO:0004252", "GO:0005524", "GO:0005737", "GO:0006508", "GO:0006515", "GO:0016887", "GO:0034605", "GO:0043565" ] }, "pscc": { "uniref50_id": "UniRef50_B1AIY7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B1AIY7" ], "product": "Lon protease" } }, "id": "NKIECMJPJD_920", "locus": "GCA000828735_00907" }, { "type": "cds", "sequence": "contig_1", "start": 530482, "stop": 531399, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAACCTATCTTAATATCACGAGCAATTGTTGTTTTACCTTATGAAACAACAACAATTGAAGTAGGACGCCCTAAATCAATCCAAGCGATTGATTTAGCTAAACAATCTTCAAGTAAAGAAATTATTATTATTTCACAAAAAGATATTGATACTGATGAAGTTGTTAATTTTGATGAATTGTATAAAGTAGGAACGTTAGTTAAAATTAAATCTATTATTGATAATTTTGATGAAGGTTATTCAATTGAAGTTGAAGGATTAAAAGCTGTTTACATTAATAATGAGAATGATGTTATTGATGCTATTGAATATGAATATGAAGATGTCATTACAAACCCTATTTTATCAAATAAAGATGAAATAGCAATCAATGGTATTAATTCTGAAATTTTCAATATAATTAACAAGCGTTCAAGACATAAAAATATCAATTTTGATAATATGCACGCTTTAATTTCACTTGAAAAAGAAAAGTTTGCTTATTTAGCTGCGGCAACTTATATTAATGATTATGATAATGAAATTAGTGAAAAAACTATTGAAGATCGAATTAATATCTTATTGCAACCTAATTTATTATTAGTTCATGAGACAATTTTACATTTATTATTTGATCAATTAGTAGATAAGCGCGTTATTGAAGATGAAGTTGAAAAAACTATTACTGATAAAATTAATAATAATTTTCAAAAACAACAACGTGAGTTCTATTTACGCGAAAAATTAAAAGTTGTTAAAGAACAATTAGGTGAACTAAGTAGTCGTGAAGAAGACGCTGATAAAATTCGTGCAAAAATTGAAGATTTAGAATTACCACCAAATGTTAAAGAACGTGCTTTAGCTGAATTAAATCGTTTTGAGAATGCTATGTCATCAAATGAATCATCAGTTATTAAATCATATCTTGATTGA", "aa": "MKKPILISRAIVVLPYETTTIEVGRPKSIQAIDLAKQSSSKEIIIISQKDIDTDEVVNFDELYKVGTLVKIKSIIDNFDEGYSIEVEGLKAVYINNENDVIDAIEYEYEDVITNPILSNKDEIAINGINSEIFNIINKRSRHKNINFDNMHALISLEKEKFAYLAAATYINDYDNEISEKTIEDRINILLQPNLLLVHETILHLLFDQLVDKRVIEDEVEKTITDKINNNFQKQQREFYLREKLKVVKEQLGELSSREEDADKIRAKIEDLELPPNVKERALAELNRFENAMSSNESSVIKSYLD", "aa_hexdigest": "a9fd54c3e14425bf78ad42a309ab7912", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 35177.49370000007, "isoelectric_point": 4.775748634338378 }, "id": "NKIECMJPJD_921", "locus": "GCA000828735_00908" }, { "type": "cds", "sequence": "contig_1", "start": 531546, "stop": 531860, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAATTTAGTAAAAAATCAGAAGCACAATTTCAAGAAGACTTGATAAGACAAGCTAAAGAAACTATAACTTTAGCACTGTTAGTTGATGATATTGCTGAAACACAAAATATTGTTGTATCTGATGAAGAAGTTAAAGAACGTGTTGCGGAAATGGCTAAACTATACCAAGGGGAAGAACAAGCTGTTATTGAACAATTATCAAAAAATCCTGATGCTGTTAAAGAGTTCTTGTTACATAAAAAAGTTGTTAATTATTTAATTGACTTAAACAAAAATAATCAGCCAAAAAATACAACTTTATCTTCAAAATAA", "aa": "MEFSKKSEAQFQEDLIRQAKETITLALLVDDIAETQNIVVSDEEVKERVAEMAKLYQGEEQAVIEQLSKNPDAVKEFLLHKKVVNYLIDLNKNNQPKNTTLSSK", "aa_hexdigest": "bcde3a53e28c2ae25ef66976e6ac2c7b", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11892.357999999986, "isoelectric_point": 4.914037895202636 }, "id": "NKIECMJPJD_922", "locus": "GCA000828735_00909" }, { "type": "cds", "sequence": "contig_1", "start": 531999, "stop": 532484, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00254.34" ], "nt": "GTGAATGAAGCTTTTGAACATGAAGTTAATGCTGAAATTGAACATGCATTAAATTCTAAATCACAAAGACGTGTTAAAGATGATAATGAATTCATTGAAAAAGGGGATGAAGTTCGATTTGATTTTAAAGGGATGATTGATAGTGTGCCTTTTGAAGGTGGAAGTGCTAAAGATCATTTATTAACAATTGGATCTAATCAATTTATTCCAGGATTTGAAGATCAAATGATAGGTTTAAAAAAAGATGAACGTAAAAATATTAATGTTAAATTTCCAGATGATTATCATGCAACCGATTTAGCAGGCAAAGCAGCAGTATTTGAAGTTTTTATCAAAGAAATTACAAATGTAAAGCCACAAGAATTAAATGATGAATTTGCTAAATCATTTAATTTGCCTAATGTTAATACAGTTCAAGAATTAAAAGATTATATTCACAATCAAATTGTTTTAGCTAAACAAGAAAAGAATAGTGAACGTGCCTGA", "aa": "MNEAFEHEVNAEIEHALNSKSQRRVKDDNEFIEKGDEVRFDFKGMIDSVPFEGGSAKDHLLTIGSNQFIPGFEDQMIGLKKDERKNINVKFPDDYHATDLAGKAAVFEVFIKEITNVKPQELNDEFAKSFNLPNVNTVQELKDYIHNQIVLAKQEKNSERA", "aa_hexdigest": "ec5e02afec53aba7ee3c2dff2b354668", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00254.34", "name": "FKBP_C", "length": 91, "aa_cov": 0.5093167701863354, "hmm_cov": 1.0, "evalue": 1.743388008318449e-18, "score": 62.31227493286133, "start": 30, "stop": 111 } ], "seq_stats": { "molecular_weight": 18427.339099999994, "isoelectric_point": 4.983608818054199 }, "id": "NKIECMJPJD_923", "locus": "GCA000828735_00910" }, { "type": "cds", "sequence": "contig_1", "start": 532600, "stop": 532851, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTCAACAGTTTCTGAATCATCATTTATAAATGATTCATCTTGTTCTAAAAAAGTTAAAACTTGCGGTATTAAATCATTTGTTGTTGTTTCAAAAACTTCAATTTCATTAACGTGTTTTTTAGCTTCTTCAACCGGAACTTTTCCTGGACGAAAACCAGGAATTTTTAATTTTGAAGCTGTTTTTTTTGCAATTTTTTCAAATGATTCTTTGTGTTTTTTCTCTCATTCTGATTTTTCAATTAAAATTTGA", "aa": "MSTVSESSFINDSSCSKKVKTCGIKSFVVVSKTSISLTCFLASSTGTFPGRKPGIFNFEAVFFAIFSNDSLCFFSHSDFSIKI", "aa_hexdigest": "49b981f263575ab226877fdde041b336", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9000.243699999992, "isoelectric_point": 8.817034721374515 }, "id": "NKIECMJPJD_924", "locus": "GCA000828735_00911" }, { "type": "cds", "sequence": "contig_1", "start": 532990, "stop": 533724, "strand": "-", "frame": 3, "gene": "scpB", "product": "SMC-Scp complex subunit ScpB, long form", "db_xrefs": [ "COG:COG1386", "COG:K", "GO:0005737", "GO:0007059", "GO:0051301", "GO:0051304", "KEGG:K06024", "SO:0001217", "UniRef:UniRef50_Q9PQE5", "UniRef:UniRef90_Q9PQE5" ], "nt": "TTGCAGAATCAATTAACCAATCATGAATTATTACGTGAAAAATTAAAACCACAAGAAGATTTTAACAAGATATTTAAAACAAAAGATATTAATGTTACTGAAGAAATTAAAATTAAGGATGTTAAAAAAAATAGTAGTGTTTCTTTTCGAAATTCATTTAATATAAATGATTTGAATCAAAATCAAATTACAAACATTAAATCAATTATTGATTCTACACTGTTTTTAGCTGGTGAAGAAGGTGTTTCTTTGCAAGATTTAAAAAGAACAACAGGATTAAATGAGAGTTCTCAACTTAAAACAATTTTAAATCAATTACAAAAAGACTATGACGTTGATGATTCGGGTTTAGTTTTAGTACAATTTGGTGAAAAATTTAAATTATTAACTCAATCCAAAAATAAAGATGCTTTATCAAAATTTGTTACAACAAGTTTTAGAACTCCCTTAAGTCAAAGAAATTTAGAAACGCTTGCTATTATTGCTTATAATCAACCAACAACTCGTGCAAAAATTCAAGCAATTCGTGATCGTGATCCCAAACCTGCCATTGACGCTCTTTTAAAATTGAATTTGATTATAGAAGCAGGCCGTCAAGACACACCAGGTCACCCAATTTTATATACTGTTAGTCAAAAATTTTATGATTTATTTGGTATAAGAAATTTAACAGAATTACCAAGATTAAATAAAGAAATAAATGAATTTAGTCCTATTGATGAATCAACTAACTAA", "aa": "MQNQLTNHELLREKLKPQEDFNKIFKTKDINVTEEIKIKDVKKNSSVSFRNSFNINDLNQNQITNIKSIIDSTLFLAGEEGVSLQDLKRTTGLNESSQLKTILNQLQKDYDVDDSGLVLVQFGEKFKLLTQSKNKDALSKFVTTSFRTPLSQRNLETLAIIAYNQPTTRAKIQAIRDRDPKPAIDALLKLNLIIEAGRQDTPGHPILYTVSQKFYDLFGIRNLTELPRLNKEINEFSPIDESTN", "aa_hexdigest": "60b4dc6c7e6eeb049eb91793265d0bce", "start_type": "TTG", "rbs_motif": null, "genes": [ "scpB" ], "truncated": "3-prime", "pseudogene": { "start": 532990, "stop": 534024, "observations": { "mutation": [ 533964 ], "stop": [ 533964 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQE5", "query_cov": 1.0, "subject_cov": 0.6703296703296703, "identity": 0.996, "score": 462.0, "evalue": 2.18e-162, "gene_start": 1, "gene_end": 244, "reference_start": 121, "reference_end": 364, "reference_sequence": "MNNDKKNKKNLLNNELDLAKYTLHNLSADNNDDENELELDWDGIDDEIIDNNQQIYQKKHINHKTRLFIEKDDKLNNHEQIESGNEIINQPKALSGQDFLEFVNSKLVQKNNNFKQNSKFLQNQLTNHELLREKLKPQEDFNKIFKTKDINVTEEIKIKDVKKNSSVSFRNSFNINDLNQNQITNIKSIIDSTLFLAGEEGVSLQDLKRTTGLNESSQLKTILNQLQKDYDVDDSGLVLVQFGEKFKLLTQSKNKDALSKFVTTSFRTPLSQRNLETLAIIAYNQPTTRAKIQAIRDRDPKPAIDALLKLNLIIEAGRQDTPGHPILYTVSQKFYDLFGIRNLTELPRLNKEINEFSPIDESTN" }, "paralog": false, "identity": 0.997093023255814, "score": 674.0, "evalue": 1.4e-248, "description": "Internal stop codon at 533964. Nonsense mutation around 533964.", "psc": { "uniref90_id": "UniRef90_Q9PQE5", "gene": "scpB", "product": "SMC-Scp complex subunit ScpB, long form", "uniref50_id": "UniRef50_Q9PQE5", "cog_id": "COG1386", "cog_category": "K", "kegg_orthology_id": "K06024", "go_ids": [ "GO:0005737", "GO:0007059", "GO:0051301", "GO:0051304" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQE5", "UniRef:UniRef50_Q9PQE5", "COG:COG1386", "COG:K", "KEGG:K06024", "GO:0005737", "GO:0007059", "GO:0051301", "GO:0051304" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQE5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQE5" ], "product": "Segregation and condensation protein B" } }, "id": "NKIECMJPJD_925", "locus": "GCA000828735_00912" }, { "type": "cds", "sequence": "contig_1", "start": 533962, "stop": 534084, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAATGATAAAAAAAATAAAAAGAATTTATTAAATAATGAATTAGATTTAGCTAAATATACATTACATAATCTATCCGCAGATAACAATGATGATGAAAATGAATTAGAATTAGATTGA", "aa": "MNNDKKNKKNLLNNELDLAKYTLHNLSADNNDDENELELD", "aa_hexdigest": "88782692e0aac2390d0fc069921e3f75", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4673.989499999999, "isoelectric_point": 4.491098976135253 }, "id": "NKIECMJPJD_926", "locus": "GCA000828735_00913" }, { "type": "cds", "sequence": "contig_1", "start": 534077, "stop": 534952, "strand": "-", "frame": 2, "gene": "scpA", "product": "Segregation and condensation protein A", "db_xrefs": [ "COG:COG1354", "COG:L", "GO:0005737", "GO:0006260", "GO:0007059", "GO:0051301", "KEGG:K05896", "RefSeq:WP_006688570.1", "SO:0001217", "UniParc:UPI000165F8C6", "UniRef:UniRef100_Q9PQE6", "UniRef:UniRef50_Q9PQE6", "UniRef:UniRef90_Q9PQE6" ], "nt": "ATGAGTGAAAAGCAAATCCAAAATAGTTTATTTGAATATAAGTTTATTGATTTTAACGGTCCATTAGATACATTGTGTGTTTTAATTAAACAAAAACGTTTAGATATTAATAATTTAGATATTTTAGAGTTGTCAAAACAATATGTTAATTTTGTAAATCAATTAATTAAAACGATCGATATTGATATTTTAGGTGACCATTTAGCAATGGCTTCTTATTTACTAGAACTTAAGACTCGAATGTTGATGCCAACTGTTGATGAAAAACAAATTATGAGTATTGAAGAAGATCGCCAAAATTTAATTGATCGTTTAATTGAATACAATGGTTATAAAAATTTAAGCGAACATTTAAAACAACGTTTTGAATTTAGAGCAACTATGATGGATTTACCACAACAAGATTATGAGCAATTTTATTTAAAAGATGCAATTTATAAGCCTTTGCCAAATCATTTAGACTCCATGATCTTAAAAAATATTATGGATAAAATCATTTATGAAAATGAATTAAAAAATTATAAAATTAATAAGATTAAAGTTCATGAATATGATGTTAAACAATTAGAACAATTGTTATTAAATTATTTAAAACAATCGCCTAATCAACAAGCAAGTATGCTTAGTTTTTTTGATATACAAGCAGTGATTGATAAAAATAAACGTTTTTTTGCAATAATGTTTTTAATTATTTTAATTTTAATTAATCGTAATGAAATTTTATTTGAAGAATTAGATAATGATTATTTATTAAAAATTAATATTGAAAGAGTTGTTGATGATTATAATTCTAGTAGTGTAGAACAAGCTAAAAATTTAACAAGTAATTTAACAAAAGATACAATTATTAATTTTAAAGGTGAAGACAATGAATAA", "aa": "MSEKQIQNSLFEYKFIDFNGPLDTLCVLIKQKRLDINNLDILELSKQYVNFVNQLIKTIDIDILGDHLAMASYLLELKTRMLMPTVDEKQIMSIEEDRQNLIDRLIEYNGYKNLSEHLKQRFEFRATMMDLPQQDYEQFYLKDAIYKPLPNHLDSMILKNIMDKIIYENELKNYKINKIKVHEYDVKQLEQLLLNYLKQSPNQQASMLSFFDIQAVIDKNKRFFAIMFLIILILINRNEILFEELDNDYLLKINIERVVDDYNSSSVEQAKNLTSNLTKDTIINFKGEDNE", "aa_hexdigest": "0839aff554c5805ce513161be21f1904", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000165F8C6", "ncbi_nrp_id": "WP_006688570.1", "uniref100_id": "UniRef100_Q9PQE6", "db_xrefs": [ "SO:0001217", "UniParc:UPI000165F8C6", "RefSeq:WP_006688570.1", "UniRef:UniRef100_Q9PQE6" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQE6", "uniref90_id": "UniRef90_Q9PQE6" }, "psc": { "uniref90_id": "UniRef90_Q9PQE6", "gene": "scpA", "product": "Segregation and condensation protein A", "uniref50_id": "UniRef50_Q9PQE6", "cog_id": "COG1354", "cog_category": "L", "kegg_orthology_id": "K05896", "go_ids": [ "GO:0005737", "GO:0051301", "GO:0007059", "GO:0006260" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQE6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQE6" ], "product": "Segregation and condensation protein A" }, "genes": [ "scpA" ], "id": "NKIECMJPJD_927", "locus": "GCA000828735_00914" }, { "type": "cds", "sequence": "contig_1", "start": 535093, "stop": 535818, "strand": "-", "frame": 3, "gene": "plsC", "product": "1-acyl-sn-glycerol-3-phosphate acyltransferase", "db_xrefs": [ "COG:COG0204", "COG:I", "SO:0001217", "UniRef:UniRef50_Q9PQE7", "UniRef:UniRef90_Q9PQE7" ], "nt": "ATGATTTTAGCGGCTATGATGGCAAATAAATATAAAAATGATCCAGAGTCTGTTAGCGTTAATGAGCGCTATAAAAAAATAATGGGTATTATTGATGCTATTTTATATTTAAAAAATATTAAAGTAGATTTGATTGGTAAAGAAAATGTTAGTATAAAAAGCATGTTATACATAGGTAATCACAAATCTAATGGTGATCCAATTGTTTTATTAAAGGCTCTTTATCAAATTAGCCCTCATAATGTGCGTTTTGTTGCTAAAATTGAATTAAAAAAATCATTTTTAAAATTTTTATTTAATTTAATTGATGTAATTTACATTGACCGTAATAATTTAAGACAATCAATTTCAACAATTAATGAACAAGTTAATGCTTTAAAACAAGATTATCGTGGATTAGTTATTTTTCCAGAAGGAACACGAAATTTTAATCATGAATTTAATGAGTTTAAATCAGGCGCTTTAACAGCTGCTTATAATACATTATCTTCGATTCAACCTTTTGTGATTTTTAACTCACAAGGTTCGTTTGAAATGAAAAACGATCAAAAGCAAAAGTTAAGATCAAATCCTTTTAAAACCCAACATATTAAAGTTAGTTTTTTAGAACCAATTCATTCTTTTAATTACATTAATATTAATAAAGATATTTTAATGCGTAAGATTCAATCAAACATTAAAAAAAATTATGATGAATTGTTAAAATCAACAATTAAAACAAATTAA", "aa": "MILAAMMANKYKNDPESVSVNERYKKIMGIIDAILYLKNIKVDLIGKENVSIKSMLYIGNHKSNGDPIVLLKALYQISPHNVRFVAKIELKKSFLKFLFNLIDVIYIDRNNLRQSISTINEQVNALKQDYRGLVIFPEGTRNFNHEFNEFKSGALTAAYNTLSSIQPFVIFNSQGSFEMKNDQKQKLRSNPFKTQHIKVSFLEPIHSFNYININKDILMRKIQSNIKKNYDELLKSTIKTN", "aa_hexdigest": "9b38e1a2f8bbaa0d31cd63a16d679f7b", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_Q9PQE7", "query_cov": 1.0, "subject_cov": 0.9128787878787878, "identity": 0.9129999999999999, "score": 437.0, "evalue": 4.3e-154, "valid": true, "gene": "PlsC", "product": "1-acyl-sn-glycerol-3-phosphate acyltransferase", "uniref50_id": "UniRef50_Q9PQE7", "cog_id": "COG0204", "cog_category": "I" }, "pscc": { "uniref50_id": "UniRef50_Q9PQE7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQE7" ], "product": "1-acyl-sn-glycerol-3-phosphate acyltransferase" }, "genes": [ "plsC" ], "id": "NKIECMJPJD_928", "locus": "GCA000828735_00915" }, { "type": "cds", "sequence": "contig_1", "start": 535939, "stop": 536226, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_006688435.1", "SO:0001217", "UniParc:UPI00000C1C29", "UniRef:UniRef100_A0AAC9T2B9", "UniRef:UniRef50_A0AAC9T2B9", "UniRef:UniRef90_A0AAC9T2B9" ], "nt": "ATGAATTTTGATATTAATTTAATTAATGAAAATGATTATGTTTTTAGTGGATACATTATTTTAAAAAAACACAATGAAGAAATTATCGAATTACTAACTTATTTAGGCGATGATCCTATTAATGATGAAGCCAAAATCATAACTTTATATAATAATGATCATATTGATCTAAAAAATCTTAATGATGGAGATTTAATTATTGTAAAAGCAAGTTTAATTATAAATAATAAAAATAACAAACAATTATTGCTTAAAGATATTAATAAACTAGAGTTAAGTATTAACTAA", "aa": "MNFDINLINENDYVFSGYIILKKHNEEIIELLTYLGDDPINDEAKIITLYNNDHIDLKNLNDGDLIIVKASLIINNKNNKQLLLKDINKLELSIN", "aa_hexdigest": "87ab8470e28c9fbdc23c837ccd297448", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "ups": { "uniparc_id": "UPI00000C1C29", "ncbi_nrp_id": "WP_006688435.1", "uniref100_id": "UniRef100_A0AAC9T2B9", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1C29", "RefSeq:WP_006688435.1", "UniRef:UniRef100_A0AAC9T2B9" ] }, "ips": { "uniref100_id": "UniRef100_A0AAC9T2B9", "uniref90_id": "UniRef90_A0AAC9T2B9" }, "psc": { "uniref90_id": "UniRef90_A0AAC9T2B9", "uniref50_id": "UniRef50_A0AAC9T2B9" }, "pscc": { "uniref50_id": "UniRef50_A0AAC9T2B9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T2B9" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10983.421999999984, "isoelectric_point": 4.559362602233888 }, "id": "NKIECMJPJD_929", "locus": "GCA000828735_00916" }, { "type": "cds", "sequence": "contig_1", "start": 536303, "stop": 536977, "strand": "-", "frame": 2, "gene": "cmk", "product": "(d)CMP kinase", "db_xrefs": [ "COG:COG0283", "COG:F", "EC:2.7.4.25", "GO:0004127", "GO:0005524", "GO:0005737", "GO:0006220", "GO:0015949", "GO:0036430", "GO:0036431", "RefSeq:WP_006688521.1", "SO:0001217", "UniParc:UPI000012DD0E", "UniRef:UniRef100_Q9PQE9", "UniRef:UniRef50_Q9PQE9", "UniRef:UniRef90_Q9PQE9" ], "nt": "ATGAAAAAATATATCAGTATCGCTATTGATGGACCTTCAGGTTCTGGAAAAAGTACAGCAGCAAAAGAATTAGCCAAGAAATTGGGATTTTTATATATTAACACTGGTTTAATGTATCGTGCTTATGCTTATTTTTTATCTATTAATAAATTAGATATTAATGTTAACGAAATGGATTGTATTAATGCCATTAAAAATGCTAATTTTATTTTTAATGGTGATAATGTGAAAATTAATGACAACGATGTTAGTCTTATCTTAAGAAGTAATGATGTGGCAATGATTGCTAGTGTTGTATCTGCTAATGAAAAAATTCGAGATTTAGCAACTAATGAACAACGCAGAATTGCTAGTGAAAACAACGTTGTTATGGATGGGCGCGATATTGGAAGTATTGTTTTAACTAATGCTGATCTTAAATTTTATTTAAATACGAGCATTCAAACACGCGCTAAACGACGTTTGTTTCAGAATAAAGATATTGAAAATTTGGATTATGAATCAATATATAACGATATTAAAGAACGTGATCATCGTGATATGACTCGCAAAATTGCTCCACTTAAAAAAGCAAACGATGCTGTTGAAATTTTTAACGATAACATGAAATTAGATGAATGTGTACAACATTTGTATGAAATTTATTTAAAAAAAATAAAAAAGCTAAATTTTTAA", "aa": "MKKYISIAIDGPSGSGKSTAAKELAKKLGFLYINTGLMYRAYAYFLSINKLDINVNEMDCINAIKNANFIFNGDNVKINDNDVSLILRSNDVAMIASVVSANEKIRDLATNEQRRIASENNVVMDGRDIGSIVLTNADLKFYLNTSIQTRAKRRLFQNKDIENLDYESIYNDIKERDHRDMTRKIAPLKKANDAVEIFNDNMKLDECVQHLYEIYLKKIKKLNF", "aa_hexdigest": "82b475fa51afafc767ee13b88fc73df0", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI000012DD0E", "ncbi_nrp_id": "WP_006688521.1", "uniref100_id": "UniRef100_Q9PQE9", "db_xrefs": [ "SO:0001217", "UniParc:UPI000012DD0E", "RefSeq:WP_006688521.1", "UniRef:UniRef100_Q9PQE9" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQE9", "uniref90_id": "UniRef90_Q9PQE9" }, "psc": { "uniref90_id": "UniRef90_Q9PQE9", "gene": "cmk", "product": "(d)CMP kinase", "ec_ids": [ "2.7.4.25" ], "uniref50_id": "UniRef50_Q9PQE9", "cog_id": "COG0283", "cog_category": "F", "go_ids": [ "GO:0004127", "GO:0005524", "GO:0005737", "GO:0006220", "GO:0015949", "GO:0036430", "GO:0036431" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQE9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQE9" ], "product": "Cytidylate kinase" }, "genes": [ "cmk" ], "id": "NKIECMJPJD_930", "locus": "GCA000828735_00917" }, { "type": "cds", "sequence": "contig_1", "start": 536977, "stop": 537357, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAP9AD06", "UniRef:UniRef90_A0AAC9T3L7" ], "nt": "ATGACACCATATGTTATTTCACAAGTTCAAGATCTGGTTAGTGTTTATGATGTAAGACAAATTAGTGAAATAATTCTTGAATTAAATCAAGTTAATATTCAAATTCAAACTAAAGATAGTGAGTTTTATATTAAAAATAATAAAAAATTAAGTTTAATTAGTAGTGAATTTGAAGATTATCAATTAAAAAATTATGATGCATATGAACATTTTAAAATTACAATGGATGAACCTAAAATTCATACTTTAAATCATCAAATGCCATTTGATTCAAAATTAAACATCAAAACCATCATTTTTATAGTTTTAACTTCTTGTTTATTATGTTTATTAATCGTCATAATTATATGTGCATTATTAATTGCAGGAGTTATTAATTAA", "aa": "MTPYVISQVQDLVSVYDVRQISEIILELNQVNIQIQTKDSEFYIKNNKKLSLISSEFEDYQLKNYDAYEHFKITMDEPKIHTLNHQMPFDSKLNIKTIIFIVLTSCLLCLLIVIIICALLIAGVIN", "aa_hexdigest": "b959a57a1b0809b8595573e7342cdfd3", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 536977, "stop": 537552, "observations": { "mutation": [ 537423 ], "stop": [ 537423 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAC9T3L7", "query_cov": 1.0, "subject_cov": 0.6596858638743456, "identity": 1.0, "score": 229.0, "evalue": 5.85e-75, "gene_start": 1, "gene_end": 126, "reference_start": 66, "reference_end": 191, "reference_sequence": "MIDRKQIHVKTRLTINELNKFYTLIRVQKQSLKEIEDKIKKLDWINFSEIKNQNLFDEFKFDYKNMTPYVISQVQDLVSVYDVRQISEIILELNQVNIQIQTKDSEFYIKNNKKLSLISSEFEDYQLKNYDAYEHFKITMDEPKIHTLNHQMPFDSKLNIKTIIFIVLTSCLLCLLIVIIICALLIAGVIN" }, "paralog": false, "identity": 0.9947643979057592, "score": 372.0, "evalue": 8.48e-134, "description": "Internal stop codon at 537423. Nonsense mutation around 537423.", "psc": { "uniref90_id": "UniRef90_A0AAC9T3L7", "uniref50_id": "UniRef50_A0AAP9AD06", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAC9T3L7", "UniRef:UniRef50_A0AAP9AD06" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAP9AD06", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAP9AD06" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 14609.003399999978, "isoelectric_point": 5.224890327453611 }, "id": "NKIECMJPJD_931", "locus": "GCA000828735_00918" }, { "type": "cds", "sequence": "contig_1", "start": 537421, "stop": 537552, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATAGATCGTAAACAAATTCATGTTAAAACTAGATTAACAATTAATGAGTTAAATAAGTTTTATACTTTAATTAGAGTCCAAAAACAAAGCTTAAAAGAGATTGAAGATAAAATCAAAAAACTTGATTGA", "aa": "MIDRKQIHVKTRLTINELNKFYTLIRVQKQSLKEIEDKIKKLD", "aa_hexdigest": "aab7a97437fe4eb51db0e7868d81fb01", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5269.2147, "isoelectric_point": 9.89874973297119 }, "id": "NKIECMJPJD_932", "locus": "GCA000828735_00919" }, { "type": "cds", "sequence": "contig_1", "start": 537589, "stop": 538275, "strand": "-", "frame": 3, "gene": "ybhB", "product": "YbhB/YbcL family Raf kinase inhibitor-like protein", "db_xrefs": [ "COG:COG1881", "COG:R", "SO:0001217", "UniRef:UniRef50_B5ZBF0", "UniRef:UniRef90_A0AAC9T0J3" ], "nt": "GTGCATCATAAGCTATCAACAAACCGAAACAATATTTTATATTTAGACGATTTTGAAGCGAAATTAAATGAAGCAAAAATTATTGCCACAGGTTCTACTTATGTTTGTGCTCCACAGATTAGTTTTGAAGATGATAAATTAATTATTGGTTCATTAGAGCATAAACATCCTTATTTGTTTGCTAATTGTGAAAATGAATATCATGTAATAGAAGATGTAATTGTTACAGATATTAATAAGAATATTTATAGTGAAAATCAACTAGATAATTTTAAAGATCTAGAACAAGCAAAAATTAAAATTATTGATTCTAGTGATGCTTTATCGTATGCAGTTATAATTACTTCAAATCATCTATTTAAAAAATTTGGTCGTCCAGTTGTTAATTATGCACACGTTATCGAGAAAAAAACTAATAAACGAATTAAGTTCAATAATTCCTACAAATTAAATCATGCCTTTAGCGAAATTAGTCCTTCAAAATATGATCATTTAGGTAATGAATTATTGATTTTAGAAGATAAAGATGCTAATTTTGAAAGTGGACTATTCACAATTCATGTGTTTGGATTAAATAAAAAAATTAAGTATTTAAATAAGATTGATAGTGTTGCAAATTCAATTAGTGATTTATTTGAAATGATTAGCGGTAGTGTTATTTCTTATAAAGCGAAGTTATTTAAGTAA", "aa": "MHHKLSTNRNNILYLDDFEAKLNEAKIIATGSTYVCAPQISFEDDKLIIGSLEHKHPYLFANCENEYHVIEDVIVTDINKNIYSENQLDNFKDLEQAKIKIIDSSDALSYAVIITSNHLFKKFGRPVVNYAHVIEKKTNKRIKFNNSYKLNHAFSEISPSKYDHLGNELLILEDKDANFESGLFTIHVFGLNKKIKYLNKIDSVANSISDLFEMISGSVISYKAKLFK", "aa_hexdigest": "5a29b481eced82c425a4d4690d734c04", "start_type": "GTG", "rbs_motif": null, "genes": [ "ybhB" ], "truncated": "3-prime", "pseudogene": { "start": 537589, "stop": 538575, "observations": { "mutation": [ 538482, 538563 ], "stop": [ 538482, 538563 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAC9T0J3", "query_cov": 1.0, "subject_cov": 0.5876288659793815, "identity": 0.996, "score": 448.0, "evalue": 7.95e-157, "gene_start": 1, "gene_end": 228, "reference_start": 161, "reference_end": 388, "reference_sequence": "MYKLIFNLCIYIKNKGKFEYMVENKFLKQIKEGEQIKIEIKGLDKFNNFDEQNYGLNNISPQIRWSPVLETASYAVAIIDYEAVGGAPFVHWYALNIFEPELALNVANLGYANLYQGENSTSKHFSHSILDANYQTDIANNYFPPCPPNKSHKYEIKVYAVHHKLSTNRNNILYLDDFEAKLNEAKIIATGSTYVCAPQISFEDDKLIIGSLEHKHPYLFANCENEYHVIEDVIVTDINKNIYSENQLDNFKDLEQAKIKIIDSSDALSYAVIITSNHLFKKFGRPVVNYAHVIEKKTNKRIKFNNSYKLNHAFSEISPSKYDHLGNELLILEDKDANFESGLFTIHVFGLNKKIKYLNKIDSVANSISDLFEMISGSVISYKAKLFK" }, "paralog": false, "identity": 0.9939024390243902, "score": 654.0, "evalue": 3.06e-240, "description": "Internal stop codon at 538482, 538563. Nonsense mutation around 538482, 538563.", "psc": { "uniref90_id": "UniRef90_A0AAC9T0J3", "gene": "YbhB", "product": "YbhB/YbcL family Raf kinase inhibitor-like protein", "uniref50_id": "UniRef50_B5ZBF0", "cog_id": "COG1881", "cog_category": "R", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAC9T0J3", "UniRef:UniRef50_B5ZBF0", "COG:COG1881", "COG:R" ] }, "pscc": { "uniref50_id": "UniRef50_B5ZBF0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B5ZBF0" ], "product": "YbhB/YbcL family Raf kinase inhibitor-like protein" } }, "id": "NKIECMJPJD_933", "locus": "GCA000828735_00920" }, { "type": "cds", "sequence": "contig_1", "start": 538561, "stop": 538695, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTAGAAAATAAATTTTTAAAGCAAATTAAAGAGGGAGAGCAAATTAAAATTGAAATTAAAGGTTTGGATAAATTTAATAATTTTGACGAACAAAATTATGGTTTAAATAATATTTCACCTCAAATTCGTTGA", "aa": "MVENKFLKQIKEGEQIKIEIKGLDKFNNFDEQNYGLNNISPQIR", "aa_hexdigest": "d2ff669671335d8fb1d0a1f2bbed7076", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5224.898400000001, "isoelectric_point": 6.133177375793457 }, "id": "NKIECMJPJD_934", "locus": "GCA000828735_00921" }, { "type": "cds", "sequence": "contig_1", "start": 538805, "stop": 539932, "strand": "-", "frame": 2, "gene": "dnaK", "product": "molecular chaperone DnaK", "db_xrefs": [ "COG:COG0443", "COG:O", "GO:0005524", "GO:0006457", "GO:0016887", "GO:0051082", "GO:0140662", "SO:0001217", "UniRef:UniRef50_O87777", "UniRef:UniRef90_Q9PQF2" ], "nt": "ATGGCTATGCAACGTTTAAAAGATGCTGCTGAAAAAGCTAAAATTGAATTATCAGGTATAAACACAACAACTATTTCATTACCTTTTATTGCAATGGATAGTTCTGGTCAGCCAATTAATTTTGAAAAAGAATTAAATCGAGCGACATTTGATAATCTAACTAAAAATTTAATTGAAAGATTAAAAAAACCTGTTTTAGATGCAATGAAAGAATCTAAATTATCATTGGTTGATATTGATCAAGTTTTGATGGTAGGTGGTTCAACACGTATGCCTGCAGTACAAAATTTAGTGAAAGAATTAACAGGTAAAGAACCAAACCATTCACTAAATCCTGATGAAGTTGTTGCAATTGGTGCAGCTATTCAAGGTGGAGTATTAGCAGGAGAAATTGATGATATTCTATTACTTGATGTAACACCACTTACTTTATCAATTGAAACAATGGGAGGTGTAGCAACTCCTTTAATTCCAAGAAACACAAAGATTCCAGTAAGTAAATCACAAATTTTTTCAACAGCTGCTGATAATCAACCAAGTGTTGATATAAGAATTGTGCAAGGTGAACGTTCATTAGCTGCTGATAATAAATTATTAGGTAATTTTGAATTAAGCGGAATTGAACCAGCTCCACGTGGTGTTCCGCAAATTGAAATTAAGTTTAATATTGATGCTAATGGGATCATGAGTGTTAACGCTAAAGATTTAAAAACACAAAAAGAAACATCAATCACAATTAAAGATTCACAAGGATTAAGTCAAGATGAAATTGACAAAATGATTAAGGAAGCTGAGGAAAACAAAGAAAAAGATGCTAAAGTAAAACATGAACGTGAACTTGTAAATCGTGCTGATAGTCTAATTAACCAATTAGAACAAGTGTCAAAAACTGAAAATGTTCCACAAGAACAAAAAGATGTATTTAACAAACAAATTGAAGATTTAACAAACGCACGCGATGCTCAAGATTATGTAAAACTAGAAGCTGAAGTTAAAAAAGTTGAAGATTTATTAACTAATGCTGCTAAATTTGCTCAACAAGCTCAACAGCAAAATCCAGATAATCAAAACAATAATAAAGATGATGTTACTGAAGCAACTGTAACTGATGATTCAACAAAGAAATAA", "aa": "MAMQRLKDAAEKAKIELSGINTTTISLPFIAMDSSGQPINFEKELNRATFDNLTKNLIERLKKPVLDAMKESKLSLVDIDQVLMVGGSTRMPAVQNLVKELTGKEPNHSLNPDEVVAIGAAIQGGVLAGEIDDILLLDVTPLTLSIETMGGVATPLIPRNTKIPVSKSQIFSTAADNQPSVDIRIVQGERSLAADNKLLGNFELSGIEPAPRGVPQIEIKFNIDANGIMSVNAKDLKTQKETSITIKDSQGLSQDEIDKMIKEAEENKEKDAKVKHERELVNRADSLINQLEQVSKTENVPQEQKDVFNKQIEDLTNARDAQDYVKLEAEVKKVEDLLTNAAKFAQQAQQQNPDNQNNNKDDVTEATVTDDSTKK", "aa_hexdigest": "a2cf852c5ca23bc4682c8ea4d86a3e82", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_R6RJX7", "query_cov": 0.9706666666666667, "subject_cov": 0.9732620320855615, "identity": 0.569, "score": 365.0, "evalue": 1.25e-121, "valid": false, "product": "Chaperone protein DnaK", "uniref50_id": "UniRef50_R6RJX7" }, "genes": [ "dnaK" ], "truncated": "both", "pseudogene": { "start": 538794, "stop": 540232, "observations": { "deletion": [ 538816 ], "mutation": [ 539986, 540007 ], "stop": [ 539986, 540007 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQF2", "query_cov": 1.0, "subject_cov": 0.6208609271523179, "identity": 0.9570000000000001, "score": 660.0, "evalue": 1.33e-234, "gene_start": 1, "gene_end": 375, "reference_start": 229, "reference_end": 600, "reference_sequence": "MAKEIILGIDLGTTNSCVAVIENKKPIVLENPEGKRTVPSVVSFNGDEVLVGDAAKRKQITNPNTVSSIKRLMGTKEKVTILNKEYTPEEISAKILSYIKDYAEKKLGTKINKAVITVPAYFDDAQRQATKNAGIIAGLTVERIINEPTAAALAYGIDKLDKEQKILVFDLGGGTFDVSVLDMADGTFEVLSTSGDNHLGGDDWDQVIINWLLKSIADEFNIDLSKNKMAMQRLKDAAEKAKIELSGVNTTTISLPFIAMDSSGQPINFEKELNRATFDNLTKNLIERLKKPVLDAMKESKLSLADIDQVLMVGGSTRMPAVQNLVKELTGKEPNHSLNPDEVVAIGAAIQGGVLAGEIDDILLLDVTPLTLSIETMGGVATPLIPRNTKIPVSKSQVFSTAADNQPSVDIRIVQGERSLAADNKLLGNFELSGIEPAPRGVPQIEIKFNIDANGIMSVNAKDLKTQKETSITIKDSQGLSQEEIDKMIKEAEENKEKDAKVKHERELVNRADSLINQLEQVVKTENVPQEQKDAFNKQIEELTNARDAQDYTKLEAEVKKVEDLLANAAKFAQQTQQQDPNNQKDDVTEATVTDDSNKKIINF" }, "paralog": false, "identity": 0.9561586638830898, "score": 868.0, "evalue": 4.83e-319, "description": "Internal stop codon at 539986, 540007. Frameshift due to deletion around 538816. Nonsense mutation around 539986, 540007.", "psc": { "uniref90_id": "UniRef90_Q9PQF2", "gene": "dnaK", "product": "molecular chaperone DnaK", "uniref50_id": "UniRef50_O87777", "cog_id": "COG0443", "cog_category": "O", "go_ids": [ "GO:0005524", "GO:0006457", "GO:0016887", "GO:0051082", "GO:0140662" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQF2", "UniRef:UniRef50_O87777", "COG:COG0443", "COG:O", "GO:0005524", "GO:0006457", "GO:0016887", "GO:0051082", "GO:0140662" ] }, "pscc": { "uniref50_id": "UniRef50_O87777", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_O87777" ], "product": "Chaperone protein DnaK" } }, "id": "NKIECMJPJD_935", "locus": "GCA000828735_00922" }, { "type": "cds", "sequence": "contig_1", "start": 540005, "stop": 540616, "strand": "-", "frame": 2, "gene": "dnaK", "product": "Chaperone protein DnaK", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_W7C0L0" ], "nt": "ATGGCAAAAGAAATTATTTTAGGAATTGATTTGGGTACAACAAATTCATGTGTAGCCGTAATTGAAAATAAAAAACCAATTGTTTTAGAGAATCCAGAAGGAAAACGAACAGTTCCATCTGTAGTTTCATTTAACGGCGATGAAGTTTTAGTAGGTGATGCTGCAAAACGTAAACAAATAACTAATCCAAATACTATTTCATCAATCAAACGTTTAATGGGTACAAAAGAAAAAGTAACTGTTTTAAATAAAGATTATACTCCAGAAGAGATTTCAGCAAAAATTTTAACTTATATTAAAGAGTATGCAGAAAAGAAAATTGGAGCAAAAGTTAATAAGGCTGTAATTACAGTTCCAGCATATTTTGATGATGCACAACGTCAAGCTACAAAAAATGCAGGAATTATTGCAGGATTGAGCGTTGAAAGAATTATTAATGAACCAACAGCTGCAGCTTTAGCTTATGGAATTGATAAATTAGATAAAGAACAAAAAATATTGGTATTTGATTTAGGTGGTGGTACATTTGATGTATCAGTTTTAGATATGGCAGATGGTACATTTGAAGTATTATCAACAAGTGGTGATAACCATTTAGGTGGTGATGATTGA", "aa": "MAKEIILGIDLGTTNSCVAVIENKKPIVLENPEGKRTVPSVVSFNGDEVLVGDAAKRKQITNPNTISSIKRLMGTKEKVTVLNKDYTPEEISAKILTYIKEYAEKKIGAKVNKAVITVPAYFDDAQRQATKNAGIIAGLSVERIINEPTAAALAYGIDKLDKEQKILVFDLGGGTFDVSVLDMADGTFEVLSTSGDNHLGGDD", "aa_hexdigest": "6610a457ef2d51b84259f29e0a80181a", "start_type": "ATG", "rbs_motif": "AGGAG/GGAGG", "psc": { "uniref90_id": "UniRef90_A0A7Y0YRM2", "query_cov": 0.9753694581280788, "subject_cov": 0.8181818181818182, "identity": 0.6970000000000001, "score": 275.0, "evalue": 5.42e-91, "valid": false, "product": "Chaperone protein DnaK", "uniref50_id": "UniRef50_W7C0L0" }, "pscc": { "uniref50_id": "UniRef50_W7C0L0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_W7C0L0" ], "product": "Chaperone protein DnaK" }, "genes": [ "dnaK" ], "id": "NKIECMJPJD_936", "locus": "GCA000828735_00923" }, { "type": "cds", "sequence": "contig_1", "start": 541467, "stop": 542678, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B5ZBE8", "UniRef:UniRef90_A0A2C9DXX8" ], "nt": "ATGATTGACAAGCAAAAAATTAAACAACTAATCCAAAATCCTGTTTATGGATTTTTTGATGAATTTAAAAAAAATTTAAACAGCGATCTTGATCATGTAATTTTAGAACAATTAAAAGATCAAGATAATAATATTGATAAAATTAATCATTTAGTAACCGATATTAATAATTTAAAAAATGATATTAAACAACGCAAAAAAGATGAAAAAATTAAACAACGGACTTTTTGTAATATTCTAATCGCAGTTTGTTTTGTTATTATTATTGGTTTGTTTTTTTTACATTTTTATTTGAAAAATTCCAAATTTATTAAAAATTTTAAAAAGTATGAAATTGAAAAAAATAATCAAATTAATATATGTAACAGCCAAAAAAATAAATATATTTATAATGTTTTATCACAAATTAATTCTTATAAAATTTCGCAAGAAATTTTTGCTAAGAATGGTTTATATTTAATGCCAGAAATTCAATATGAAAATATTAAACAAACCGATTTATTAATTTCACGAAACGCAAAATTTGTTGGATTTTTTGGTGGTTTAGAAGTTAAATTTAAAAGTACGGATACTTTTTTAGTGTATTATAAGGAATTTTCAATTAAAGATGTTGTAACAAGTGGTTGTATTCAAATTTCTTATCGAAAAGGAGATCAAATGGTTAGTGAAACGATCGTTGCTCATCATAAGGAACCAACACCGTTTGTTGATCTTCACAGTAATTTTGTTCATAAAACTAATTACTCTCCAAACTTTAACTTTTCAACGTACGCTCAAACTGTTAATTCTCGTTCAAAAACAATTTTTTCTAATTTAGAATTTTCTAAGTATTATGGCTTAGGAATTGCTACTCAAAATTTAGAATTTGATACAAAAATGCTAGAATTTTTTACGCCTTTTAGTCAAGAAAACTATGAAAATTTTGCTAAATATTTAAAACAAGATGATATCACAGTTCCTATGTTTACAAAAACTGCGACATCCCTTATTATTGATGATCAATTATTAAAAGATAATAGAAAACCATTCTTTATAAATTTATTAACAAATAATAGTTATGTAAAAACTTGTGATTTTCTAATTGATACAGATATTAATGCGGACCTACAAACGAATTTAAATATTTTAAAATATGAATTAGTAAAATTATTTGAAAAATATATTTTATATTTAACTACAACAAGTTTATCAAGTGTAATTGCCCGAGAATGA", "aa": "MIDKQKIKQLIQNPVYGFFDEFKKNLNSDLDHVILEQLKDQDNNIDKINHLVTDINNLKNDIKQRKKDEKIKQRTFCNILIAVCFVIIIGLFFLHFYLKNSKFIKNFKKYEIEKNNQINICNSQKNKYIYNVLSQINSYKISQEIFAKNGLYLMPEIQYENIKQTDLLISRNAKFVGFFGGLEVKFKSTDTFLVYYKEFSIKDVVTSGCIQISYRKGDQMVSETIVAHHKEPTPFVDLHSNFVHKTNYSPNFNFSTYAQTVNSRSKTIFSNLEFSKYYGLGIATQNLEFDTKMLEFFTPFSQENYENFAKYLKQDDITVPMFTKTATSLIIDDQLLKDNRKPFFINLLTNNSYVKTCDFLIDTDINADLQTNLNILKYELVKLFEKYILYLTTTSLSSVIARE", "aa_hexdigest": "366fc645c38ab254ee94c3afaa4e9de7", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 541164, "stop": 542678, "observations": { "mutation": [ 541469 ], "stop": [ 541469 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A2C9DXX8", "query_cov": 1.0, "subject_cov": 0.64171974522293, "identity": 1.0, "score": 755.0, "evalue": 3.77e-271, "gene_start": 1, "gene_end": 403, "reference_start": 1, "reference_end": 403, "reference_sequence": "MIDKQKIKQLIQNPVYGFFDEFKKNLNSDLDHVILEQLKDQDNNIDKINHLVTDINNLKNDIKQRKKDEKIKQRTFCNILIAVCFVIIIGLFFLHFYLKNSKFIKNFKKYEIEKNNQINICNSQKNKYIYNVLSQINSYKISQEIFAKNGLYLMPEIQYENIKQTDLLISRNAKFVGFFGGLEVKFKSTDTFLVYYKEFSIKDVVTSGCIQISYRKGDQMVSETIVAHHKEPTPFVDLHSNFVHKTNYSPNFNFSTYAQTVNSRSKTIFSNLEFSKYYGLGIATQNLEFDTKMLEFFTPFSQENYENFAKYLKQDDITVPMFTKTATSLIIDDQLLKDNRKPFFINLLTNNSYVKTCDFLIDTDINADLQTNLNILKYELVKLFEKYILYLTTTSLSSVIAREWYINNDTYKIGDNYDYESYYYTNQNQLTPLSVSTKLFLKNQIAFVNDCEVIPFAELVNNQTRFGINKAQFNIKSYHVYNRVDNVPVYAHGRTHIVPVQYKEYIEYEYPFICLYVLKYKQLTEEIGLKYFTRPFNNILLTNFEDHSACSIDEILDFNDNESKNNSDNDFKIYLKELDTIVTNANLNKNEFSFLKDNDGYFITISNKIELDEETEQELSTWLRKVSL" }, "paralog": false, "identity": 0.998015873015873, "score": 983.0, "evalue": 0.0, "description": "Internal stop codon at 541469. Nonsense mutation around 541469.", "psc": { "uniref90_id": "UniRef90_A0A2C9DXX8", "uniref50_id": "UniRef50_B5ZBE8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A2C9DXX8", "UniRef:UniRef50_B5ZBE8" ] }, "pscc": { "uniref50_id": "UniRef50_B5ZBE8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B5ZBE8" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 47324.77590000002, "isoelectric_point": 8.726843452453611 }, "id": "NKIECMJPJD_937", "locus": "GCA000828735_00924" }, { "type": "cds", "sequence": "contig_1", "start": 542755, "stop": 543564, "strand": "-", "frame": 3, "gene": "folD", "product": "methenyltetrahydrofolate cyclohydrolase", "db_xrefs": [ "COG:COG0190", "COG:H", "RefSeq:WP_006688679.1", "SO:0001217", "UniParc:UPI00000C1C25", "UniRef:UniRef100_Q9PQF4", "UniRef:UniRef50_Q9PQF4", "UniRef:UniRef90_Q9PQF4" ], "nt": "ATGAAACTAAAAATTGATTTTTTATACGAAAAAGTCTTAACAGAATTATTAACTAAAAAAAACAAAACTAAAAAACGTATTTATGTTTTTTATGATTCAGATTTAGTAAATTTTAATAGTGCATATATTCAATCAAAATTAAAATATGCTAAATTATTTTCTGTTAAACTTATTATTTATGATTTAAATGCATACCAAATTCAAGAAAATCATCATTTTTTATCAAAACTTAAACAAGCAAATAAGGATTTTTTGTTTTTTGAATTCCCTTTGTCAAAAAAACATTTGGATCAAAATTATTTTCAATATTTAACTTTTCAAAATGATCTCGATGGAATTAATTCACATTTGTTTTTAAATAAAAAACAAGAAAATCAAATTATTTACCATCCAGCAACAATTAATGCAATTTTAACATTAATTAAAGAATTAGATGTAAATTTAAATGACTATCGAATTTGCATTATTGGTCGTTCACCCTATTTAGGGGATTATTTATATAAGTTATTAAATCCTAAAAATAATAAAATTAATATTATTCATTCACAAACTAAAAATCCATTAAGAATTATTAAAGAATCTAATTTTATTATTAGCTGTGTGAATAAAGCACACATTTTTAATTCTAATGCATTAAGTGATAATTCTTATTTAATTGATGTTACTACAGAATACATCAATAATAAGTTATGTGGTTCTTTTTTAATTAATAAAAATGATGCATTAAATCGAAACATTAAATACACTCCTGTACCAGGTGGAATCGGTAAATTAACGACGTTATTTTTATTTTTGAATTATTTTAAATAA", "aa": "MKLKIDFLYEKVLTELLTKKNKTKKRIYVFYDSDLVNFNSAYIQSKLKYAKLFSVKLIIYDLNAYQIQENHHFLSKLKQANKDFLFFEFPLSKKHLDQNYFQYLTFQNDLDGINSHLFLNKKQENQIIYHPATINAILTLIKELDVNLNDYRICIIGRSPYLGDYLYKLLNPKNNKINIIHSQTKNPLRIIKESNFIISCVNKAHIFNSNALSDNSYLIDVTTEYINNKLCGSFLINKNDALNRNIKYTPVPGGIGKLTTLFLFLNYFK", "aa_hexdigest": "ab997608c4967d36cd2413dd58cc9ac7", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00000C1C25", "ncbi_nrp_id": "WP_006688679.1", "uniref100_id": "UniRef100_Q9PQF4", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1C25", "RefSeq:WP_006688679.1", "UniRef:UniRef100_Q9PQF4" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQF4", "uniref90_id": "UniRef90_Q9PQF4" }, "psc": { "uniref90_id": "UniRef90_Q9PQF4", "gene": "FolD", "product": "methenyltetrahydrofolate cyclohydrolase", "uniref50_id": "UniRef50_Q9PQF4", "cog_id": "COG0190", "cog_category": "H" }, "pscc": { "uniref50_id": "UniRef50_Q9PQF4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQF4" ], "product": "methenyltetrahydrofolate cyclohydrolase" }, "genes": [ "folD" ], "id": "NKIECMJPJD_938", "locus": "GCA000828735_00925" }, { "type": "cds", "sequence": "contig_1", "start": 543622, "stop": 544050, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTATGATTTACGTGTTAAATTTATGTATGGTTTAGGTTCAGAAATTGTTATTTTAAAATTTTTAATTGAGCATTATAAAAAATTAAATAGTTTACGTTTAAGATTAGAAACATTAAACCACCCTCTTTCAAATTATGCTGTTTATTTTGATATTTTAAACCGAATTGAAAAACAAACACAATTGTTAGCAAAAATTGAAACTTTAAAATATGATAATTATGATTTTAATGCTGATGTTATTTTAGATGATTTAGAAAGTAATATCACAAAAATAAATGAGATTAAACATAATTCTAATGTTTTAAATAAAACAAAAGTTATTAAAACGATTATTGAGCCAAAATATGAATTAGAATTAATTTCAAAAGATGATAAAGTTGATGGTGTTAAGGTTTACGTTAACGATCAAGAAATTCTTTCTAAATAA", "aa": "MYDLRVKFMYGLGSEIVILKFLIEHYKKLNSLRLRLETLNHPLSNYAVYFDILNRIEKQTQLLAKIETLKYDNYDFNADVILDDLESNITKINEIKHNSNVLNKTKVIKTIIEPKYELELISKDDKVDGVKVYVNDQEILSK", "aa_hexdigest": "6e2a042f58e862089ebddd98717f24f2", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16739.193799999986, "isoelectric_point": 6.306877326965333 }, "id": "NKIECMJPJD_939", "locus": "GCA000828735_00926" }, { "type": "cds", "sequence": "contig_1", "start": 544228, "stop": 544434, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAGTAAAATCATTAATCAATGAAGATAGTTTTTTAACAAAAAGACAAGTTAAAAACAAAAATAAAATTCTTACTGAATTTGAAAAACATCGTTCACAAATTACACAAAATTATTTAAATAAACGTAGTGATATTCTCAAGAAAGAATATGATGAATTATTAAAAGATGATAAATTATTTGAGTTAAGCTCAGGCGATAATTGA", "aa": "MKVKSLINEDSFLTKRQVKNKNKILTEFEKHRSQITQNYLNKRSDILKKEYDELLKDDKLFELSSGDN", "aa_hexdigest": "60a422ef7354573f8436d632154b2f43", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8147.1923, "isoelectric_point": 9.222282981872556 }, "id": "NKIECMJPJD_940", "locus": "GCA000828735_00927" }, { "type": "cds", "sequence": "contig_1", "start": 544513, "stop": 544929, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAAAACTAATCAAATTGTGGATTTATTAACCAATAAACAAAATAATTCAATTCAAAGACGCGATAATCTAGTTTTTGTTAAAAATGAACAAGAATTCAAAGATATTTTAGATTCTACTATTAGCTTAAGTAATGTTTATGATCCTTTGTTAGGTAATGGTTGTGAACAAGAAAAAATAAAAGAATTAACTATTTTAAATAATAGTGTTGATATTAGCAAAATTGAAGAGTATACTAAAACATTAGAAAGTAAAGTAGATGATTTATACAATAATGTTTCTAACCAACTAATTCAAGATTATCAAAAAAATGAGAATACAAGTGCGAATAATATAATTATGAAAAATGAAGAATTATTAAATTCTCATTTAAAAGAAGTCGAAAAAATTTGTGAACAGCTAATTGATGAATGA", "aa": "MNKTNQIVDLLTNKQNNSIQRRDNLVFVKNEQEFKDILDSTISLSNVYDPLLGNGCEQEKIKELTILNNSVDISKIEEYTKTLESKVDDLYNNVSNQLIQDYQKNENTSANNIIMKNEELLNSHLKEVEKICEQLIDE", "aa_hexdigest": "6db41be1dbdb4d8fcd24e404faae96b1", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16007.697299999994, "isoelectric_point": 4.596762657165528 }, "id": "NKIECMJPJD_941", "locus": "GCA000828735_00928" }, { "type": "cds", "sequence": "contig_1", "start": 544975, "stop": 546285, "strand": "-", "frame": 3, "gene": null, "product": "DUF31 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T0W6", "UniRef:UniRef90_A0AAC9T0W6" ], "nt": "TTGCCGTTTTCATTTCAACGTTATGATTATGAATCAAGACGTAATTTCATTAGAAACTCACGTCAATTAGTTGATACCTCAATTGATTTTGACGATCTTGAATATGATTTTTTGCACAATAAGAAACCAAAACAAGAACTAGAAAAAAATAATGAATCAATTAAAATAGCTGAATTAAAAAAATTTGAAAATTCACAATTAAGTGATGAACAACAATCTAATTTAGAAAAAGAGTTCTATAGTATTGGAAGAATTAACTGTTTTGACGAAGTTAGCGAAAATAAACAAGAATTAAACAAAGAACAATTAGTAATCGATAAAAAAAATGGGTATTTGAAGCCACGTAGTTACTTACGTCATCATGAAACTCCATTTATGGTTGACATTGAGAACCGGGTAGACTTTTTAATGGCAGGATATGAAATTATTAAAAATGATGATCCATATGCAATTAAACAAGAAATATTAAAAAATACCATTTTAGAACAACAAAAGCTTGTCGAACAAGATGATCATGATTTTAAAGTTAAAAATGATGATCAAGAAGCAAATGAAGAATTATTGGACAATACTGATAAATTAGAAAATCAAGATTCTACAAGTGCTGTTGAATTTAATGATTCATCTGAAGTTACTCAAGTAGTAAAAACGGATGAAAATGTTGATAATTTAATTAAAACTAAGCAAATAACTAGTTTTAACGATCTTAAACAAGAGCAATCATCTCAAGAAAATATTATTAAAAATGATCTTTCATCAATTCAAATTCGTTTAGATGAAATTATTAATGTACATGAAAAAGATACTAAATTACAAACCGAACTAAATTCAATTATGATAGATAGTAATGAAAAAAATATTGATGATAGTTTAAATAATTTAAATGAACAAATACGAATTCAAGATCAAGAATTAGAATCATCAGATAATCAAAATGTAATTATTGAAGAAGAAATTAATACCAAAAATTTAAATAATAAAGCTGATGTTGGTGTTATGAGTGTTGAAAAAAAATATGAACCTGAAGAGGAAATGGTTAATTTTGAATTTGAAATACCTGAAAACCAAAAAGAATCAGTTAACCAAGATGTTTTAAATAGTAATAGTATTGATAATTTTAAAGAAGAAAAAGCTAAAGAAAATACTTTCTTAACACCAAAAGAAATAGAAAATAATGATTTATCATCACAAATTGAATTAACACCTGCTGATTTTGAACTTAAGACACAAGAAATTAATGACGAAACTGAACGTTTATTGGATGAATTAAATAATAATAAACCTAAAGAAAAGAAGAAATTTTGGACATGA", "aa": "MPFSFQRYDYESRRNFIRNSRQLVDTSIDFDDLEYDFLHNKKPKQELEKNNESIKIAELKKFENSQLSDEQQSNLEKEFYSIGRINCFDEVSENKQELNKEQLVIDKKNGYLKPRSYLRHHETPFMVDIENRVDFLMAGYEIIKNDDPYAIKQEILKNTILEQQKLVEQDDHDFKVKNDDQEANEELLDNTDKLENQDSTSAVEFNDSSEVTQVVKTDENVDNLIKTKQITSFNDLKQEQSSQENIIKNDLSSIQIRLDEIINVHEKDTKLQTELNSIMIDSNEKNIDDSLNNLNEQIRIQDQELESSDNQNVIIEEEINTKNLNNKADVGVMSVEKKYEPEEEMVNFEFEIPENQKESVNQDVLNSNSIDNFKEEKAKENTFLTPKEIENNDLSSQIELTPADFELKTQEINDETERLLDELNNNKPKEKKKFWT", "aa_hexdigest": "dfc5b22a0306bf60b346521c5a82b96b", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 544954, "stop": 546585, "observations": { "mutation": [ 544977, 546537 ], "stop": [ 544977, 546537 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAC9T0W6", "query_cov": 1.0, "subject_cov": 0.6411764705882353, "identity": 0.998, "score": 803.0, "evalue": 8.91e-289, "gene_start": 1, "gene_end": 436, "reference_start": 238, "reference_end": 673, "reference_sequence": "MFYINLRVKTMMEKLKNNKTLFLNVEQAQKLGINNPLTQEIFLDDQKLKVHLINSIKDEFYLKKSISKGYAYFNSLEQFKTMELDVLDNYQFAKIAATNLDEYDQLVYTKTRQYLINKEFWTNDGLDAFIGDDFKRKQLTSKEIVNLKTKYDLWIQELHFNNARISKQIHNLSIHGQNKLDYEKFYDKKATLESNLDILQILIYLRSSLLELDFDDLFKDDQNKISIRQLLVNDLEYLPFSFQRYDYESRRNFIRNSRQLVDTSIDFDDLEYDFLHNKKPKQELEKNNESIKIAELKKFENSQLSDEQQSNLEKEFYSIGRINCFDEVSENKQELNKEQLVIDKKNGYLKPRSYLRHHETPFMVDIENRVDFLMAGYEIIKNDDPYAIKQEILKNTILEQQKLVEQDDHDFKVKNDDQEANEELLDNTDKLENQDSTSAVEFNDSSEVTQVVKTDENVDNLIKTKQITSFNDLKQEQSSQENIIKNDLSSIQIRLDEIINVHEKDTKLQTELNSIMIDSNEKNIDDSLNNLNEQIRIQDQELESSDNQNVIIEEEINTKNLNNKADVGVMSVEKKYEPEEEMVNFEFEIPENQKESVNQDVLNSNSIDNFKEEKAKENTFLTPKEIENNDLSSQIELTPADFELKTQEINDETERLLDELNNNKPKEKKKFWTWFNSKKR" }, "paralog": false, "identity": 0.996316758747698, "score": 1062.0, "evalue": 0.0, "description": "Internal stop codon at 544977, 546537. Nonsense mutation around 544977, 546537.", "psc": { "uniref90_id": "UniRef90_A0AAC9T0W6", "product": "DUF31 domain-containing protein", "uniref50_id": "UniRef50_A0AAC9T0W6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAC9T0W6", "UniRef:UniRef50_A0AAC9T0W6" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAC9T0W6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T0W6" ], "product": "DUF31 domain-containing protein" } }, "id": "NKIECMJPJD_942", "locus": "GCA000828735_00929" }, { "type": "cds", "sequence": "contig_1", "start": 546372, "stop": 546503, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGATCTTAAATAAATTAAAATTTGAAGAATATCTAAATTTGATTCAAGGGTTGCTTTTTTATCATAAAATTTCTCATAATCTAATTTATTTTGACCATGAATACTTAAATTATGAATTTGTTTACTAA", "aa": "MKILNKLKFEEYLNLIQGLLFYHKISHNLIYFDHEYLNYEFVY", "aa_hexdigest": "767510cd1581da8a0e44cba2ca33adba", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5458.286599999999, "isoelectric_point": 6.233384513854981 }, "id": "NKIECMJPJD_943", "locus": "GCA000828735_00930" }, { "type": "cds", "sequence": "contig_1", "start": 546634, "stop": 546996, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTTATATTAATTTAAGGGTGAAAACAATGATGGAAAAATTAAAAAACAACAAGACTTTGTTTTTGAATGTAGAACAAGCTCAAAAACTAGGGATTAATAACCCTTTAACACAAGAGATTTTTTTAGATGATCAAAAGTTAAAAGTTCATTTAATTAATAGTATAAAAGATGAGTTTTACTTAAAAAAAAGTATTAGTAAAGGTTATGCTTATTTTAATAGTTTAGAACAATTTAAGACTATGGAGCTTGATGTTTTAGATAACTATCAATTTGCAAAAATAGCAGCTACTAATTTAGATGAGTATGATCAATTAGTTTACACAAAAACTCGTCAATATTTAATTAATAAAGAATTTTGA", "aa": "MFYINLRVKTMMEKLKNNKTLFLNVEQAQKLGINNPLTQEIFLDDQKLKVHLINSIKDEFYLKKSISKGYAYFNSLEQFKTMELDVLDNYQFAKIAATNLDEYDQLVYTKTRQYLINKEF", "aa_hexdigest": "cc1f27f7b6ef7a65585ca6fc745f99e0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14350.437699999984, "isoelectric_point": 8.667854881286619 }, "id": "NKIECMJPJD_944", "locus": "GCA000828735_00931" }, { "type": "cds", "sequence": "contig_1", "start": 547082, "stop": 547339, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_006688677.1", "SO:0001217", "UniParc:UPI000165F8C3", "UniRef:UniRef100_A0AAC9X6U7", "UniRef:UniRef50_A0AAC9X6U7", "UniRef:UniRef90_A0AAC9X6U7" ], "nt": "ATGAATGATAGTGTTAAACAAACCAATAATATTGATGAAAATCAATTAAAACCAACTACAAAAAGCTTTTTTGATGATAAAAACAACCAATTAACAAAAGTTTTAAATATTCCTAGCGTTGTTAAATATACGGAATCAAAAATTAAAAAAAATGGTAAAAATTTTTCAATTAATTTAATCATTACAATTACAACAATGTTTATTGTAATTGGGATTATTATAACTATTGCTATTGCTATTGGTATTAAATTAAGTTAG", "aa": "MNDSVKQTNNIDENQLKPTTKSFFDDKNNQLTKVLNIPSVVKYTESKIKKNGKNFSINLIITITTMFIVIGIIITIAIAIGIKLS", "aa_hexdigest": "87b2722beb4702ccb8a3049dac005790", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000165F8C3", "ncbi_nrp_id": "WP_006688677.1", "uniref100_id": "UniRef100_A0AAC9X6U7", "db_xrefs": [ "SO:0001217", "UniParc:UPI000165F8C3", "RefSeq:WP_006688677.1", "UniRef:UniRef100_A0AAC9X6U7" ] }, "ips": { "uniref100_id": "UniRef100_A0AAC9X6U7", "uniref90_id": "UniRef90_A0AAC9X6U7" }, "psc": { "uniref90_id": "UniRef90_A0AAC9X6U7", "uniref50_id": "UniRef50_A0AAC9X6U7" }, "pscc": { "uniref50_id": "UniRef50_A0AAC9X6U7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9X6U7" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9498.065799999991, "isoelectric_point": 9.699284553527832 }, "id": "NKIECMJPJD_945", "locus": "GCA000828735_00932" }, { "type": "cds", "sequence": "contig_1", "start": 547348, "stop": 547521, "strand": "-", "frame": 3, "gene": "rpmF", "product": "50S ribosomal protein L32", "db_xrefs": [ "COG:COG0333", "COG:J", "GO:0003735", "GO:0006412", "GO:0015934", "RefSeq:WP_006688693.1", "SO:0001217", "UniParc:UPI0000165FAF", "UniRef:UniRef100_Q9PQF8", "UniRef:UniRef50_Q9PQF8", "UniRef:UniRef90_Q9PQF8" ], "nt": "ATGGCTGTACAACAAAGAAGAGTAAGTAAATCAAGAAAAGGAATGCGTCGTAGTCACGATCACCTTACAATTTCTAATACAGTAGCATGTAATGAATGTGGTAAAGCATTACTACCTCATAGAGCTTGTCGTGATTGCAAAACTTACAGAAGTATTAAGTTATCAATCAAATAA", "aa": "MAVQQRRVSKSRKGMRRSHDHLTISNTVACNECGKALLPHRACRDCKTYRSIKLSIK", "aa_hexdigest": "6a0937f7e2a46bfe60dcf907b1e2e355", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI0000165FAF", "ncbi_nrp_id": "WP_006688693.1", "uniref100_id": "UniRef100_Q9PQF8", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000165FAF", "RefSeq:WP_006688693.1", "UniRef:UniRef100_Q9PQF8" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQF8", "uniref90_id": "UniRef90_Q9PQF8" }, "psc": { "uniref90_id": "UniRef90_Q9PQF8", "gene": "rpmF", "product": "50S ribosomal protein L32", "uniref50_id": "UniRef50_Q9PQF8", "cog_id": "COG0333", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015934" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQF8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQF8" ], "product": "Large ribosomal subunit protein bL32" }, "genes": [ "rpmF" ], "id": "NKIECMJPJD_946", "locus": "GCA000828735_00933" }, { "type": "cds", "sequence": "contig_1", "start": 547707, "stop": 547913, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF07693.20", "PFAM:PF00485.24", "PFAM:PF06414.18" ], "nt": "ATGAATACTAAATCCCCAATTCTTATACTAATTGCAGGAGCTTCTGGTTCTGGAAAAACAACCTTTGCTAATGAAATTATTGCAAGAATTCCTAAAAATACAACAAGTGTTGTTATTTGTCAAGATTCTTACTATATTTCTAATTCACAACTAAATAAAAAAGAACGCCGCTTAATTAATTATGATCATCCAAGTAGTTTTGAATGA", "aa": "MNTKSPILILIAGASGSGKTTFANEIIARIPKNTTSVVICQDSYYISNSQLNKKERRLINYDHPSSFE", "aa_hexdigest": "c8804014d727ea3dd4bc388c8bece399", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07693.20", "name": "KAP_NTPase", "length": 45, "aa_cov": 0.6617647058823529, "hmm_cov": 1.0, "evalue": 1.247692420208048e-05, "score": 20.196245193481445, "start": 3, "stop": 47 }, { "id": "PF00485.24", "name": "PRK", "length": 46, "aa_cov": 0.6617647058823529, "hmm_cov": 1.0, "evalue": 5.030288441321382e-06, "score": 22.001033782958984, "start": 9, "stop": 53 }, { "id": "PF06414.18", "name": "Zeta_toxin", "length": 42, "aa_cov": 0.6176470588235294, "hmm_cov": 1.0, "evalue": 1.5745337281597003e-07, "score": 26.538028717041016, "start": 3, "stop": 44 } ], "seq_stats": { "molecular_weight": 7546.507200000001, "isoelectric_point": 9.300869941711426 }, "id": "NKIECMJPJD_947", "locus": "GCA000828735_00934" }, { "type": "cds", "sequence": "contig_1", "start": 547920, "stop": 548195, "strand": "+", "frame": 3, "gene": "udk", "product": "uridine kinase", "db_xrefs": [ "COG:COG0572", "COG:F", "EC:2.7.1.48", "GO:0004849", "GO:0005524", "GO:0005737", "GO:0043771", "GO:0044206", "GO:0044211", "KEGG:K00876", "SO:0001217", "UniRef:UniRef50_Q9PQF9", "UniRef:UniRef90_Q9PQF9" ], "nt": "ATGCGAGAGCAATTAAGTGACATTAAAAAACGTAAAAAAATTAAAGTACCAATCTATGATTATAAAACTGAGATTCGTTTAGATAAAACGATTGATATTAGTGATGTTGATGTTATAGTTCTTGAAGGTATTTATGCAATTTATGATGATGTCATTAATCAAATAGCTGATTTGAAAATTTTTATTGAAACTCCAAAAGATGAATGTTTAATTCGACGAATTCTTCGAGATGTTAATGAACGTAATCGATCATTTGAATCTGTAATTACTCAATGA", "aa": "MREQLSDIKKRKKIKVPIYDYKTEIRLDKTIDISDVDVIVLEGIYAIYDDVINQIADLKIFIETPKDECLIRRILRDVNERNRSFESVITQ", "aa_hexdigest": "45b2f025b49765d14a63862a862ba196", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "udk" ], "truncated": "both", "pseudogene": { "start": 547707, "stop": 548330, "observations": { "mutation": [ 547911, 548193, 548268, 548310 ], "stop": [ 547911, 548193, 548268, 548310 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQF9", "query_cov": 1.0, "subject_cov": 0.4396135265700483, "identity": 1.0, "score": 171.0, "evalue": 3.02e-52, "gene_start": 1, "gene_end": 91, "reference_start": 72, "reference_end": 162, "reference_sequence": "MNTKSPILILIAGASGSGKTTFANEIIARIPKNTTSVVICQDSYYISNSQLNKKERRLINYDHPSSFEWELMREQLSDIKKRKKIKVPIYDYKTEIRLDKTIDISDVDVIVLEGIYAIYDDVINQIADLKIFIETPKDECLIRRILRDVNERNRSFESVITQWRSTVSPMYDQFVEPSKKNANVSVLWNEHNRVALHLIHKWINNIH" }, "paralog": false, "identity": 0.9806763285024155, "score": 393.0, "evalue": 2.39e-142, "description": "Internal stop codon at 547911, 548193, 548268, 548310. Nonsense mutation around 547911, 548193, 548268, 548310.", "psc": { "uniref90_id": "UniRef90_Q9PQF9", "gene": "udk", "product": "uridine kinase", "ec_ids": [ "2.7.1.48" ], "uniref50_id": "UniRef50_Q9PQF9", "cog_id": "COG0572", "cog_category": "F", "kegg_orthology_id": "K00876", "go_ids": [ "GO:0004849", "GO:0005524", "GO:0005737", "GO:0043771", "GO:0044206", "GO:0044211" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQF9", "EC:2.7.1.48", "UniRef:UniRef50_Q9PQF9", "COG:COG0572", "COG:F", "KEGG:K00876", "GO:0004849", "GO:0005524", "GO:0005737", "GO:0043771", "GO:0044206", "GO:0044211" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQF9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQF9" ], "product": "Uridine kinase" } }, "id": "NKIECMJPJD_948", "locus": "GCA000828735_00935" }, { "type": "cds", "sequence": "contig_1", "start": 548545, "stop": 548661, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGACCCTAAAAATAAAGGTTATTATTTTTTAGTTGATAAATACACAAATACAATTATTTCTTACACTAAACGAACTAATAAAAATGATTTTTACCATATTAAAAAAATTAAATAA", "aa": "MDPKNKGYYFLVDKYTNTIISYTKRTNKNDFYHIKKIK", "aa_hexdigest": "e711bf0ab10ee7e4ef3530092c3256a5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4690.3793000000005, "isoelectric_point": 9.795277976989745 }, "id": "NKIECMJPJD_949", "locus": "GCA000828735_00936" }, { "type": "cds", "sequence": "contig_1", "start": 549399, "stop": 549731, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTTGTTGAAGCTTTTGTTATCATTTATTATTTACGTAATAGAAATCGTTCAAAAGTACAGTTAATTAATTTTATTTATTGCTCATTATTAGTAATTTTTTCAACAATGTTATTTTCGTTTGCACTAGGACCTTACGTAAGTGTAGCGTATTTAAAATATATAAAAGTCCCAATTACGAATTATTTAAAATACGGAGTTAATTACTATTTATTACCACGCATTATTAAAGAAAGCATTAAAACACCAATTTATATCATTTTATTATCTTCAATAATTAGTGCATTTGATTATGTCTTTCTAAATATTAAGCAAATTAATATGCACAAATGA", "aa": "MLVEAFVIIYYLRNRNRSKVQLINFIYCSLLVIFSTMLFSFALGPYVSVAYLKYIKVPITNYLKYGVNYYLLPRIIKESIKTPIYIILLSSIISAFDYVFLNIKQINMHK", "aa_hexdigest": "5ddd7168fabfa81d7b7e657b4907d71f", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12992.540699999985, "isoelectric_point": 9.743767738342285 }, "id": "NKIECMJPJD_950", "locus": "GCA000828735_00937" }, { "type": "cds", "sequence": "contig_1", "start": 549780, "stop": 550010, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATGCTATTCAAGAACCTATTGTAGGCTTTATTGCTGGATTTCTAGGATCTGTAGCTACATTAAGAAAAAAAACTAAACGACCAATAATTGATCTAATAGTTAATCAAATCTTTATTTTATTTTTTAGTATATTAACATTGTTTATGATTTTGACCTTAGTTAATGATGGCAAAAACTCCTTATTTAGCATTCTTAAAAAACGTGATAAAGAAATTAATGAAGATTGA", "aa": "MYAIQEPIVGFIAGFLGSVATLRKKTKRPIIDLIVNQIFILFFSILTLFMILTLVNDGKNSLFSILKKRDKEINED", "aa_hexdigest": "7123f0a9ecae0af4597d74d6d8c99d38", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8674.305999999993, "isoelectric_point": 9.520900535583493 }, "id": "NKIECMJPJD_951", "locus": "GCA000828735_00938" }, { "type": "cds", "sequence": "contig_1", "start": 550119, "stop": 550373, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATTCAAGATTTAAATTTAACTATGATATAAATAATAAATTAGCATTATCCTTTACAAAGGATTTAATTGCTAATAAAAAGCAAAAAAATAAAAAAAAATTTTTGCTTTTTTTATTTCCCTTTTATCCTTTAAAACTTTTTAAAACGATTAATTTAATCGTGGTTTTTGCTGTTTTAGTTGCAATTCGTTTAGGAATGGGATTTTTACATATTAAAATTCCTGGTTTTAATATGGTTATTTCTTTTACATGA", "aa": "MNSRFKFNYDINNKLALSFTKDLIANKKQKNKKKFLLFLFPFYPLKLFKTINLIVVFAVLVAIRLGMGFLHIKIPGFNMVISFT", "aa_hexdigest": "62db853b511d16d04ca22ba8fc0cbb54", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9832.900099999992, "isoelectric_point": 10.551749992370606 }, "id": "NKIECMJPJD_952", "locus": "GCA000828735_00939" }, { "type": "cds", "sequence": "contig_1", "start": 550374, "stop": 550760, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTGGAGCATTATTAGTAGTTGCATTAGCAAATATTTTTACTGGAAATTTAAGATTTTCAAGTAGTTCATTTGATGAAATGTTTCGTCAAGCACAAATTAATGATGTATTAGCTAACTTAGCAAGAAATAATAGTTTTAGTTTTGATTATTCGTATTTACAAAGTATTTTTTATGGCTATAGTATCGCCCAAATTTTAGTATTTGTAGTTATTATTTTAGGAACAATTTTAATTATTAGTACTTTTATTTTAAATCCTAAACGTGATATTCAAAGAATAAAATTATTATTACAAGAAATTCATGATGATATTTTAACAGGTAAAATTGATCCTAATACATATTTAAGAACAAAAAATCCCTTTAGAGACTTGTTTGGTTTTTAG", "aa": "MFGALLVVALANIFTGNLRFSSSSFDEMFRQAQINDVLANLARNNSFSFDYSYLQSIFYGYSIAQILVFVVIILGTILIISTFILNPKRDIQRIKLLLQEIHDDILTGKIDPNTYLRTKNPFRDLFGF", "aa_hexdigest": "eee3a781a1bdf064dcd012e7ab8623c3", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14664.868799999975, "isoelectric_point": 8.028650856018068 }, "id": "NKIECMJPJD_953", "locus": "GCA000828735_00940" }, { "type": "cds", "sequence": "contig_1", "start": 550767, "stop": 551249, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGTTTTTATACTCCAAGAAATCAAGAAAATAATACTAGTACAGAATATGAAAAGGTAAGTGCTGAATCTATTAGTGATGAAGATAGTATGAATCATACTTATCAACCAACTTCTGATTTTACAACAGCAAATATCGATAATTTAACAAATATGGTATTTAGTGCTCGGGTTATGAATGGTGAAATTTATTTATATGAAACGAAACCAAAAGTTATTCCTTTCTTAAAATATGTTTTTTTTGCTGTTAGTTTAGCTTTGGTTTTTTTTACAACATTAACGTATGCTTTTCTAATGGATTTTGGCACACATATGTCAAGTGTTATTAATGTTAATGGTATTACTCATATTGTTCGTTTAGTTTCGCCAACATTTCCAACCCAGTTAGTGATATCAATATTCATTATGATTTATGCTGGAATAAATGCTTTTAAAAGAAAAATTAGTGAAAATGAGAAGTTTCATTTTAAAAATTTTATTTGA", "aa": "MGFYTPRNQENNTSTEYEKVSAESISDEDSMNHTYQPTSDFTTANIDNLTNMVFSARVMNGEIYLYETKPKVIPFLKYVFFAVSLALVFFTTLTYAFLMDFGTHMSSVINVNGITHIVRLVSPTFPTQLVISIFIMIYAGINAFKRKISENEKFHFKNFI", "aa_hexdigest": "fd124882352c52d83ad91ce32150efdc", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18378.8515, "isoelectric_point": 6.053091239929199 }, "id": "NKIECMJPJD_954", "locus": "GCA000828735_00941" }, { "type": "cds", "sequence": "contig_1", "start": 551436, "stop": 551642, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTAAATGCAGAATTGTCAAGTGCACAAAAAGATTTATATAAATCAATTAAATTATATTTAAACACAATTTTTTTGAATCAAAAACTAAATTATGCTATTAAAAATGATAATGATTATCGAAATCAATTAAATAAAGTAATGAATATTTTTGAATATTATGAAGATAATTATGAAATAGCAAATAAAAATTTTCATAGTTATTGA", "aa": "MLNAELSSAQKDLYKSIKLYLNTIFLNQKLNYAIKNDNDYRNQLNKVMNIFEYYEDNYEIANKNFHSY", "aa_hexdigest": "49e2e79e23d6a63b11a4a31a89db7f21", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8252.174299999999, "isoelectric_point": 6.539405632019044 }, "id": "NKIECMJPJD_955", "locus": "GCA000828735_00942" }, { "type": "cds", "sequence": "contig_1", "start": 551814, "stop": 552380, "strand": "-", "frame": 1, "gene": "holA", "product": "DNA polymerase III subunit delta", "db_xrefs": [ "COG:COG1466", "COG:L", "EC:2.7.7.7", "GO:0003677", "GO:0003887", "GO:0006260", "SO:0001217", "UniRef:UniRef50_Q9PQG3", "UniRef:UniRef90_Q9PQG3" ], "nt": "ATGACATATACAAATGAAAATGCTATGGCTTTCTTAAAAGAAGAGTTAAATCAGCGTAATTTATCATTAAATAAATCTATAATGCAAACGATTATTCAAAAAACAAATTTTAATATTAATTTTCTTAATAATGAATTAGATAAAATAGAATTAATTAATGATTTTTTAAAAAATCATGGTATGTTTGATATTAATAATTTTATATGTGATTATGGTGAATATCAAATTTTTAGTTTATTAAATCTTTTGTATCAACATAAAATAAATGAATCAATAAATTTAATTAATAAAATGCTCATTGACAAAATTGATGAACTCACAATAATTAATATGTTAGCGACAATAATGAGTACACATTACTTAATTAAATTATTACATGAAAAAAAATATAACAAAAACGTTATATCTAGCTCTTTAAATCAAAAACCTTATATTATTGATTTAAATTTAAAATTAATTAGAAATTATTCAGCTAAATTTTTACTTAAAAAAATGTATGATTTATTACAATTAGAAATTAAAGTCAAGGAAAATAAAATTGATAAATATTTTGGATTAATTAATTGA", "aa": "MTYTNENAMAFLKEELNQRNLSLNKSIMQTIIQKTNFNINFLNNELDKIELINDFLKNHGMFDINNFICDYGEYQIFSLLNLLYQHKINESINLINKMLIDKIDELTIINMLATIMSTHYLIKLLHEKKYNKNVISSSLNQKPYIIDLNLKLIRNYSAKFLLKKMYDLLQLEIKVKENKIDKYFGLIN", "aa_hexdigest": "47fd851bec0485ec8b2d5cc6505c21ea", "start_type": "ATG", "rbs_motif": null, "genes": [ "holA" ], "truncated": "both", "pseudogene": { "start": 551799, "stop": 552680, "observations": { "mutation": [ 551816, 552539 ], "stop": [ 551816, 552539 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQG3", "query_cov": 1.0, "subject_cov": 0.5987261146496815, "identity": 1.0, "score": 357.0, "evalue": 9.72e-123, "gene_start": 1, "gene_end": 188, "reference_start": 122, "reference_end": 309, "reference_sequence": "MNLINKELFLITGDNRLKMDQKINEIATSFDELVKINDQEISLISFKNLIEQDDLFNSNKIYLFKNVNWFENLEHLKNVSDLIDYFFNNHVAIIITIESTKISTAKKIQETIAKFNHQITFMTYTNENAMAFLKEELNQRNLSLNKSIMQTIIQKTNFNINFLNNELDKIELINDFLKNHGMFDINNFICDYGEYQIFSLLNLLYQHKINESINLINKMLIDKIDELTIINMLATIMSTHYLIKLLHEKKYNKNVISSSLNQKPYIIDLNLKLIRNYSAKFLLKKMYDLLQLEIKVKENKIDKYFGLINWVLNF" }, "paralog": false, "identity": 0.9897610921501706, "score": 565.0, "evalue": 3.07e-207, "description": "Internal stop codon at 551816, 552539. Nonsense mutation around 551816, 552539.", "psc": { "uniref90_id": "UniRef90_Q9PQG3", "gene": "holA", "product": "DNA polymerase III subunit delta", "ec_ids": [ "2.7.7.7" ], "uniref50_id": "UniRef50_Q9PQG3", "cog_id": "COG1466", "cog_category": "L", "go_ids": [ "GO:0003677", "GO:0003887", "GO:0006260" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQG3", "EC:2.7.7.7", "UniRef:UniRef50_Q9PQG3", "COG:COG1466", "COG:L", "GO:0003677", "GO:0003887", "GO:0006260" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQG3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQG3" ], "product": "DNA-directed DNA polymerase" } }, "id": "NKIECMJPJD_956", "locus": "GCA000828735_00943" }, { "type": "cds", "sequence": "contig_1", "start": 552537, "stop": 552743, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACTTAATAAATAAAGAACTTTTTTTAATTACTGGTGACAATCGTTTAAAAATGGATCAAAAAATTAATGAAATTGCGACCTCTTTTGATGAATTAGTTAAAATTAATGATCAAGAAATTTCATTAATTTCTTTTAAAAATCTTATTGAACAAGGTGATTTATTCAATAGTAATAAGATCTACTTATTTAAAAACGTTAACTGA", "aa": "MNLINKELFLITGDNRLKMDQKINEIATSFDELVKINDQEISLISFKNLIEQGDLFNSNKIYLFKNVN", "aa_hexdigest": "694b9fb59afbd120b88db35a1db10b2a", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7950.058199999995, "isoelectric_point": 4.88976764678955 }, "id": "NKIECMJPJD_957", "locus": "GCA000828735_00944" }, { "type": "cds", "sequence": "contig_1", "start": 552727, "stop": 553500, "strand": "-", "frame": 3, "gene": "spoU", "product": "rRNA methylase", "db_xrefs": [ "COG:COG0566", "COG:J", "RefSeq:WP_006688821.1", "SO:0001217", "UniParc:UPI00000C1C1D", "UniRef:UniRef100_Q9PQG4", "UniRef:UniRef50_A0AAP9AA20", "UniRef:UniRef90_Q9PQG4" ], "nt": "ATGTTAAAAATTATTACATCTAATAAAAATGAAGAAATTATTCATTTTTATCGTTTAATTCATGATAAAAAATATCGCGAAGCTAATAAATTATTCATAATTGAGGGTTTTAAATTATTAGATGAAGCTTTGCAAAATCAACAAACAATAGTTTCAATTATTATTAATGAACATGTTTATAATAAAGAATTTGAAACGATTTTTAAATATACATTATATGACAATATTCAAATTTATAAAGTTAGTGGAAATATTATTAAAAAACTATCAGTTAATATCGAACCAAGTCCAATTTTAGCAGTTGTTAAAATAAAACAAGAAATGTGTGATATTAACGATAATATTTTATTGTTAGATAATATTCAAGATCCAGGTAATTTAGGAACCATTTTAAGAACCTGCTTTGCTTTCAATATTAAACAAGTTATTTTTAATAATTGTGTTGATTTATATAATCCTAAAACAATTAAAGCAAGTATGGGGGCCATTTTTAAAATTAATTTTTTACGTTTTAATGATTCTAAACTAGCATTAAATTTTTTATTAAAAAATAAATTCTATTTAATTGCTACTATTTTAGATAAAAACGCAATTTCTTTAAACGAAATAACGATTAAACAAAAATATTGTTTAATGGTAGGTAATGAAGGTCATGGTTTGGTACAAACTTTTATTGATCATGCTCACATTAAAACAATGATTAAAATGAATCAAACAGAATCATTAAATGTTGCAGTAGCAGCAGCAATTTTTTTATATGAACTTAATAAATAA", "aa": "MLKIITSNKNEEIIHFYRLIHDKKYREANKLFIIEGFKLLDEALQNQQTIVSIIINEHVYNKEFETIFKYTLYDNIQIYKVSGNIIKKLSVNIEPSPILAVVKIKQEMCDINDNILLLDNIQDPGNLGTILRTCFAFNIKQVIFNNCVDLYNPKTIKASMGAIFKINFLRFNDSKLALNFLLKNKFYLIATILDKNAISLNEITIKQKYCLMVGNEGHGLVQTFIDHAHIKTMIKMNQTESLNVAVAAAIFLYELNK", "aa_hexdigest": "dcb907d59f6fae2badf92eb3ec8ab0f5", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00000C1C1D", "ncbi_nrp_id": "WP_006688821.1", "uniref100_id": "UniRef100_Q9PQG4", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1C1D", "RefSeq:WP_006688821.1", "UniRef:UniRef100_Q9PQG4" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQG4", "uniref90_id": "UniRef90_Q9PQG4" }, "psc": { "uniref90_id": "UniRef90_Q9PQG4", "gene": "SpoU", "product": "rRNA methylase", "uniref50_id": "UniRef50_A0AAP9AA20", "cog_id": "COG0566", "cog_category": "J" }, "pscc": { "uniref50_id": "UniRef50_A0AAP9AA20", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAP9AA20" ], "product": "RNA methyltransferase" }, "genes": [ "spoU" ], "id": "NKIECMJPJD_958", "locus": "GCA000828735_00945" }, { "type": "cds", "sequence": "contig_1", "start": 553504, "stop": 553863, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_006688471.1", "SO:0001217", "UniParc:UPI00000C1C1C", "UniRef:UniRef100_A0AAC9T0Y0", "UniRef:UniRef50_B5ZBD6", "UniRef:UniRef90_A0AAC9T0Y0" ], "nt": "ATGGAAAAGACAATAATTCAATTGGAATTTAATGAAGAAGAATTAGCATCAATAAAAAAAATTAAAGATAAAATGGATCCTAATAATAATTTAGAATTAAATGTATTTTTAAAAGATTTAATTAAGGATTTAGCGCGTGATTATTTAAATTTTTCAAACCAATCATTTGAAAGTATTGCAAAGCAAATGAATGATTTAAAAGATCTAATAGGAAACGTAGCGAACGATCCATCTAGTTTCGATTTTAATTCAGTAATGAATGAATTCCAAAAGTTTAGTAAATCACAAAAGGATGAAGAAAAAGAAATAAAAAACGATACTGAAATAAAAACTGCGACTAAACCTACTAAAAAATCTTAA", "aa": "MEKTIIQLEFNEEELASIKKIKDKMDPNNNLELNVFLKDLIKDLARDYLNFSNQSFESIAKQMNDLKDLIGNVANDPSSFDFNSVMNEFQKFSKSQKDEEKEIKNDTEIKTATKPTKKS", "aa_hexdigest": "a322ace84484d5ffeda17d8bbe431290", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI00000C1C1C", "ncbi_nrp_id": "WP_006688471.1", "uniref100_id": "UniRef100_A0AAC9T0Y0", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1C1C", "RefSeq:WP_006688471.1", "UniRef:UniRef100_A0AAC9T0Y0" ] }, "ips": { "uniref100_id": "UniRef100_A0AAC9T0Y0", "uniref90_id": "UniRef90_A0AAC9T0Y0" }, "psc": { "uniref90_id": "UniRef90_A0AAC9T0Y0", "uniref50_id": "UniRef50_B5ZBD6" }, "pscc": { "uniref50_id": "UniRef50_B5ZBD6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B5ZBD6" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13813.42549999998, "isoelectric_point": 4.968830680847168 }, "id": "NKIECMJPJD_959", "locus": "GCA000828735_00946" }, { "type": "cds", "sequence": "contig_1", "start": 554273, "stop": 554539, "strand": "+", "frame": 2, "gene": "lemA", "product": "LemA family protein", "db_xrefs": [ "COG:COG1704", "COG:M", "KEGG:K03744", "SO:0001217", "UniRef:UniRef50_A0A084F1M3", "UniRef:UniRef90_A0AAC9T3D1" ], "nt": "ATGATTGAACAAACTAAATCATATTACAAATATGAAGAAAAAGTATTAGATGAAATAACTAAAAATCGAAGTATTCAAATGTCAAATGATATTAACAAAAATGAAGAAGTATTAAATAAACTTCAAAATTTAGTTAATGTTCAATTCGAACGTTATCCAGATTTGAAATCATCCAATATTTTAATGGAATTGATGAGTACATCAAGTTATTTAGAAAATGAAATTGCTTCATCACGACGTGCTTATAACTCACGTGCAACTGATTGA", "aa": "MIEQTKSYYKYEEKVLDEITKNRSIQMSNDINKNEEVLNKLQNLVNVQFERYPDLKSSNILMELMSTSSYLENEIASSRRAYNSRATD", "aa_hexdigest": "2bd33dda5aad92faee38fb26030790fb", "start_type": "ATG", "rbs_motif": null, "genes": [ "lemA" ], "truncated": "both", "pseudogene": { "start": 554009, "stop": 554668, "observations": { "mutation": [ 554018, 554147, 554165, 554168, 554537 ], "stop": [ 554018, 554147, 554165, 554168, 554537 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAC9T3D1", "query_cov": 1.0, "subject_cov": 0.4018264840182648, "identity": 1.0, "score": 159.0, "evalue": 9.57e-48, "gene_start": 1, "gene_end": 88, "reference_start": 89, "reference_end": 176, "reference_sequence": "MDLWKEQDENGISPNVSNERKVVVASNGEKTLYIFIIVISIISIIGWIFLLVWWFNTKNHLIQTKNSVNEASSSIQVAQTKRFDLLNKMIEQTKSYYKYEEKVLDEITKNRSIQMSNDINKNEEVLNKLQNLVNVQFERYPDLKSSNILMELMSTSSYLENEIASSRRAYNSRATDWNIMIFQFMTVIVAEKLKLETFPIYAANKQERKDVDMKSLSDF" }, "paralog": false, "identity": 0.9771689497716894, "score": 402.0, "evalue": 1.03e-145, "description": "Internal stop codon at 554018, 554147, 554165, 554168, 554537. Nonsense mutation around 554018, 554147, 554165, 554168, 554537.", "psc": { "uniref90_id": "UniRef90_A0AAC9T3D1", "gene": "LemA", "product": "LemA family protein", "uniref50_id": "UniRef50_A0A084F1M3", "cog_id": "COG1704", "cog_category": "M", "kegg_orthology_id": "K03744", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAC9T3D1", "UniRef:UniRef50_A0A084F1M3", "COG:COG1704", "COG:M", "KEGG:K03744" ] }, "pscc": { "uniref50_id": "UniRef50_A0A084F1M3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A084F1M3" ], "product": "LemA family protein" } }, "id": "NKIECMJPJD_960", "locus": "GCA000828735_00947" }, { "type": "cds", "sequence": "contig_1", "start": 554546, "stop": 554668, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTTTTCAATTTATGACTGTTATCGTTGCTGAAAAATTAAAACTTGAAACTTTCCCAATTTATGCTGCAAACAAACAAGAGCGTAAAGATGTAGATATGAAATCACTGTCAGATTTTTAA", "aa": "MIFQFMTVIVAEKLKLETFPIYAANKQERKDVDMKSLSDF", "aa_hexdigest": "aff05fe5fd67c25591fded06cdc847e1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4726.5340000000015, "isoelectric_point": 6.060707664489747 }, "id": "NKIECMJPJD_961", "locus": "GCA000828735_00948" }, { "type": "cds", "sequence": "contig_1", "start": 554786, "stop": 556585, "strand": "+", "frame": 2, "gene": "lepA", "product": "translation elongation factor 4", "db_xrefs": [ "COG:COG0481", "COG:J", "EC:3.6.5.n1", "GO:0003746", "GO:0003924", "GO:0005525", "GO:0005886", "GO:0043022", "GO:0045727", "GO:1990606", "RefSeq:WP_006688644.1", "SO:0001217", "UniParc:UPI000012E493", "UniRef:UniRef100_Q9PQG7", "UniRef:UniRef50_Q9ZHZ8", "UniRef:UniRef90_Q9PQG7" ], "nt": "ATGGATAAAAAATTCATCCGTAATTTTAGTATTATTGCCCATATTGATCATGGGAAGTCAACATTATCTGATCGAATTATTGAATTTACAAATACTTTGAGTAAACGTGAAATGACAAACCAAATTTTAGATTCTATGGATATAGAACGTGAACGTGGAATTACTATTAAATTAAATGCTGTACAAATTAAATATCATGCTAAAGATAAACATGAATATTTAATTCACTTAATTGACACTCCCGGTCACGTCGATTTTACTTACGAAGTTTCACGAAGTTTAGCAGCATGTGAGGGAGCAATTCTAGTTGTTGATGCTGCACAAGGAATTGAAGCCCAAACTTTATCTAATGTTTACTTAGCATTAGAAAACAATTTAGAGATTGTTCCAACAATTAATAAAATTGATTTACCATCAGCAGACCCTAATCGTGTTAAAAAAGAAATCGAAGACGTTATTGGTTTAGATACAGCGGATGTGCCTTTAATTTCAGCAAAAACGGGGTTAAACATTCAAGATGTATTAGAAGCAATAATTAAACACGTTCCACCTCCATTAGATGCTAAAGATGATGCTAAATTACAAGCCTTAATATTTGATTCTTTTTATGATTCTTATAAAGGTGTTGTTTGTTTAGTACGTGTAAAACAAGGAACAATTAAAGTTGGAGATAAAATTCGTATGATGGCTAACAATAAAGATTATATTGTTAGTGAATTAGGAATTAGAACACCAAAAATTGTTAATAAAACAGAATTGGTAGCCGGAGAAGTTGGTTGGGTAGCTGCAGCTATCAAAACAGTCAAAGATATTAATGTGGGTGATACTATTACTCATACCAATAATCCTGCTGATAAACCACTACCAGGTTATAAAAAAATTTTACCAATGGTTTATTGTGGCTTATACCCAATTGATACTAGTCAATATGATGATTTAAAAGAAGCTATGGCTAAAATCTCCTTATCTGATGCTGCATTAACTTATGAATATGAAACATCGCAAGCACTAGGATTTGGAATTCGTTGTGGATTTTTAGGTTTATTACATATGGATGTTATTCGTGAACGAATTGCTCGTGAATTTAATATTGAATTAATTTTAACTGCCCCTTCAGTAATATATAAAATTGAATTAACAAATAATCAAGAAATTAGTATTGATAGTCCTGCTAAAATGCCTGAACCAACTAGTATTAAACTTATTAAAGAACCATTTGTTAAATTAGCAATCATTACACCAGATAATTATGTTGGTGCAATTATGGAATTATGTCAGTCCCGAAGAGGAATATATGATGATCTAGAAGTTATTGACGGTACAAGACGAAAATTAATTTATAAAATGCCATTAGCTGAAATTATGTACAGTTTTTTTGATTCACTAAAATCAATTACTAAAGGATATGCAACAATGGACTATGAACTAATTGGTTACCAAGCTGAAAAATTAGTCAAAATCGATATTATGTTAAATGGTAATAAAGTTGATGCTTTAAGTATTATTGCTCATCGTGATTTTGCTTATGGGAAGTCAAAAATTATTTGTGAACGTCTAAAAGAGGTTATTCCAAAACATCAATTTGAAATTCCTATTCAAGCTTCAATTGGTTCAAAAATTATTGCTCGTGAAACTATTAAAGCAGTACGCAAGGATGTGATTGCTAAATGCTATGGTGGAGATGTTTCAAGAAAGAAAAAACTTCTAGAACAACAAAAAGAAGGTAAAAAACGTCTAAAAGCGATTGGTAATGTTGATGTTCCTCAAGATGCTTTTGTAAAAGTTTTAAGTGAAAACTAA", "aa": "MDKKFIRNFSIIAHIDHGKSTLSDRIIEFTNTLSKREMTNQILDSMDIERERGITIKLNAVQIKYHAKDKHEYLIHLIDTPGHVDFTYEVSRSLAACEGAILVVDAAQGIEAQTLSNVYLALENNLEIVPTINKIDLPSADPNRVKKEIEDVIGLDTADVPLISAKTGLNIQDVLEAIIKHVPPPLDAKDDAKLQALIFDSFYDSYKGVVCLVRVKQGTIKVGDKIRMMANNKDYIVSELGIRTPKIVNKTELVAGEVGWVAAAIKTVKDINVGDTITHTNNPADKPLPGYKKILPMVYCGLYPIDTSQYDDLKEAMAKISLSDAALTYEYETSQALGFGIRCGFLGLLHMDVIRERIAREFNIELILTAPSVIYKIELTNNQEISIDSPAKMPEPTSIKLIKEPFVKLAIITPDNYVGAIMELCQSRRGIYDDLEVIDGTRRKLIYKMPLAEIMYSFFDSLKSITKGYATMDYELIGYQAEKLVKIDIMLNGNKVDALSIIAHRDFAYGKSKIICERLKEVIPKHQFEIPIQASIGSKIIARETIKAVRKDVIAKCYGGDVSRKKKLLEQQKEGKKRLKAIGNVDVPQDAFVKVLSEN", "aa_hexdigest": "efba3de470c87a405a530d1dbd673c59", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI000012E493", "ncbi_nrp_id": "WP_006688644.1", "uniref100_id": "UniRef100_Q9PQG7", "db_xrefs": [ "SO:0001217", "UniParc:UPI000012E493", "RefSeq:WP_006688644.1", "UniRef:UniRef100_Q9PQG7" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQG7", "uniref90_id": "UniRef90_Q9PQG7" }, "psc": { "uniref90_id": "UniRef90_Q9PQG7", "gene": "lepA", "product": "translation elongation factor 4", "ec_ids": [ "3.6.5.n1" ], "uniref50_id": "UniRef50_Q9ZHZ8", "cog_id": "COG0481", "cog_category": "J", "go_ids": [ "GO:0003746", "GO:0003924", "GO:0005525", "GO:0005886", "GO:0043022", "GO:0045727", "GO:1990606" ] }, "pscc": { "uniref50_id": "UniRef50_Q9ZHZ8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9ZHZ8" ], "product": "Elongation factor 4" }, "genes": [ "lepA" ], "id": "NKIECMJPJD_962", "locus": "GCA000828735_00949" }, { "type": "cds", "sequence": "contig_1", "start": 556598, "stop": 556846, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAAGTTTTGTTAAAGAATTAAATTTTGAAATTAATCAAAAAAAATATTTTATTAAAATTTATTTACAACCAAGAATTAAATATATTAAAATCAAAATCATTAATCATCAAATTGTTTGTTTGATCAATCACTTAAAATTAATTAATATTGCTGAAGAATTTATTAAAAATAATAAGTTAAAAATTTTAAAAATATATGAAAATGACTTAAAGAAAATTAAGTATGATGAACAATCTTTAGATTGA", "aa": "MLSFVKELNFEINQKKYFIKIYLQPRIKYIKIKIINHQIVCLINHLKLINIAEEFIKNNKLKILKIYENDLKKIKYDEQSLD", "aa_hexdigest": "0915a95fad009a602945605414581ed1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10037.95529999999, "isoelectric_point": 9.54804172515869 }, "id": "NKIECMJPJD_963", "locus": "GCA000828735_00950" }, { "type": "cds", "sequence": "contig_1", "start": 557896, "stop": 558141, "strand": "-", "frame": 3, "gene": "rbfA", "product": "30S ribosome-binding factor RbfA", "db_xrefs": [ "COG:COG0858", "COG:J", "GO:0005829", "GO:0006364", "GO:0030490", "GO:0043024", "KEGG:K02834", "SO:0001217", "UniRef:UniRef50_Q9PQH0", "UniRef:UniRef90_Q9PQH0" ], "nt": "ATGGCAAACGAAGTTCGAGTTGCAAGGTTAGAATCTTTAATTAAAGATGTTATTAATAATGCGTTAGCGAATGAAATTAATGATAAAATTGCAAAATTAGCACGTGTAACTGCAGTGCGTTTAAGTAATGATTTATCAGTAGCAAAGATTTTTTTAGACGCTCATAAACGCGAAAGTATGCTTAAAGTTTTAGAAAATGTTAATAAAGTTAGTGGCTTATTGCGTTCAAAATTAGCTGCGGAATGA", "aa": "MANEVRVARLESLIKDVINNALANEINDKIAKLARVTAVRLSNDLSVAKIFLDAHKRESMLKVLENVNKVSGLLRSKLAAE", "aa_hexdigest": "283c59ce929c0fa36c07a2d3f257d6e9", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "genes": [ "rbfA" ], "truncated": "5-prime", "pseudogene": { "start": 557791, "stop": 558141, "observations": { "mutation": [ 557898 ], "stop": [ 557898 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQH0", "query_cov": 1.0, "subject_cov": 0.6982758620689655, "identity": 1.0, "score": 148.0, "evalue": 8.03e-45, "gene_start": 1, "gene_end": 81, "reference_start": 1, "reference_end": 81, "reference_sequence": "MANEVRVARLESLIKDVINNALANEINDKIAKLARVTAVRLSNDLSVAKIFLDAHKRESMLKVLENVNKVSGLLRSKLAAEWTSYKVPELRFVIDETIDYANHIDELFKKIKQQEN" }, "paralog": false, "identity": 0.9913793103448276, "score": 216.0, "evalue": 3.11e-74, "description": "Internal stop codon at 557898. Nonsense mutation around 557898.", "psc": { "uniref90_id": "UniRef90_Q9PQH0", "gene": "rbfA", "product": "30S ribosome-binding factor RbfA", "uniref50_id": "UniRef50_Q9PQH0", "cog_id": "COG0858", "cog_category": "J", "kegg_orthology_id": "K02834", "go_ids": [ "GO:0005829", "GO:0006364", "GO:0030490", "GO:0043024" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQH0", "UniRef:UniRef50_Q9PQH0", "COG:COG0858", "COG:J", "KEGG:K02834", "GO:0005829", "GO:0006364", "GO:0030490", "GO:0043024" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQH0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQH0" ], "product": "Ribosome-binding factor A" } }, "id": "NKIECMJPJD_964", "locus": "GCA000828735_00951" }, { "type": "cds", "sequence": "contig_1", "start": 558149, "stop": 558481, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03144.31" ], "nt": "ATGAAAGGTACATTAGACCCAATTATTATTGAAGAAGTGATTGGCGAAGCCAAAGTTCTTAAATTATTTAAACACAGTCAAGTTGGAACAATTTGTGGTTGTCGAGTAATTAATGGTAAAATTAAAAGAAATGCTTTGGTTCGTGTTTTACGTGATGGTATTGTTATATATAATTCAAAAATTGCGACATTACAACACAATAAAGATAGTGTTAATGAAGTTATTGCTGATAAAGAATGCGGATTAACAATTGCAAACTTTAATGATATTAAAGAGAACGATATTATTGAAGTTTATATTAAAGTTGAAAAGAAACATGATGAGGTTAAATAA", "aa": "MKGTLDPIIIEEVIGEAKVLKLFKHSQVGTICGCRVINGKIKRNALVRVLRDGIVIYNSKIATLQHNKDSVNEVIADKECGLTIANFNDIKENDIIEVYIKVEKKHDEVK", "aa_hexdigest": "c35aae2f9064afb53beb8df58b8a6420", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03144.31", "name": "GTP_EFTU_D2", "length": 66, "aa_cov": 0.5545454545454546, "hmm_cov": 0.9848484848484849, "evalue": 1.6705004112239985e-09, "score": 33.62324523925781, "start": 29, "stop": 89 } ], "seq_stats": { "molecular_weight": 12356.331999999988, "isoelectric_point": 8.42590503692627 }, "id": "NKIECMJPJD_965", "locus": "GCA000828735_00952" }, { "type": "cds", "sequence": "contig_1", "start": 558482, "stop": 559993, "strand": "-", "frame": 2, "gene": "infB", "product": "translation initiation factor IF-2", "db_xrefs": [ "COG:COG0532", "COG:J", "GO:0003743", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0005829", "GO:0006413", "KEGG:K02519", "SO:0001217", "UniRef:UniRef50_Q9PQH1", "UniRef:UniRef90_Q9PQH1" ], "nt": "ATGGCGAAAAAAAATATCAAACAAAAGAAAGATAATCGGATAGCAATCGATGTAAAAAAACACATTAAAAAAGTTGATGTTGGGGTTTTTGATGGTACATTTGTTTTCACATCACCTCTTAGTATTTCAGAATTAGCACCAAAACTAAATAAAAGTCCAAACGAAATTATTATGCGTTATTTCAAAAAAGGTGTTGTTTATAACTTAAACACTATTTTAGATGAAGAACAAATTGGTGAGTTATGTTTAGAATATGATTTAGATTTTAAAATTGAAAAGAATGTTAACACTGAAAATCTTTTAGAAAATATTTATTTTGATGATCTAGAAATTGATTTGGTTGCTCGTGCTCCAATTGTAACAATTATGGGTCATGTTGATCATGGTAAAACAACACTTTTAGATACTATTCGTAAATCATCAATAACAGCATCAGAAGCTGGAGGAATTACTCAACATATTGGAGCTTATCAAATTATAAAAGACAATAGAGCAATTACTTTTATTGATACACCCGGACATGAAGCCTTTACTGAAATGCGTGCTCGCGGAGCAAATTTAACAGATATTGTGATTTTAGTTGTTGCTGCTGATGATGGTATTAAAATGCAAACTGAAGAAGCAATTGATCATGCTAAAGCTGCTAATGTTCCTATTATTGTTTTTGTTAATAAAATGGATAAATACGAAGCAAATCCTGAAAAAGTTTTAAATCAATTAAGTGCTAAAGAAATTGTTGCTGAAGAGCTTGGTGGTGATGTTGTTTTTGTTAAAGGCTCAGCATTGAAAAATGAAGGAATTTCTGAATTATTAGATTCAATTTTATTGATTGCTGAATTAAATAATTACAAAGCTAATCCTAATCGTTTAGCTTATGGAACTACAATTGAAGCAAATTTGGATAAAGGTCATGGTCCTCTTGCTACATTGTTAGTTCAAAATGGCACATTACGAAAAGGTGATTATTTAGTTGTTGGTTCAACATATGGAAAAATTCGTAATATGTTTGATGAATATGACAACGAAATTGAAATAGCATTACCTTCAAAACCTGTTAAAGTTTCTGGATTTGAAGAAGTACCAACAGCCGGTGATAAATTTTTAGCTTTAGCTGATGAAAAACAAGCACGTGCAATAGCAAATGATGTTAAACAAAAGAAAATGCGTTTAGAACGTGCAATGTTACAAAGTTCGGATATTCGAACAAAAATTGCTAATGGTGAATTAAAAAATATTAATTTAATTATTAAAGCTGATGTTCAAGGTTCACTTGAAGCTTTAAAAGGAATTTTTAGTAGCATTAACATTGAAGGTGTGACTACGACACTAATACGGAGTGCAATTGGAACAATTAGTGAAAGTGATGTGCGTTTAGCACAGACTTCTGATGCAATAATTATTGGTTTTAATGTCCGTGCTAGTCGAATTATTAAAGATTTAGCTGATTCAGTCGGTGTACAAATTATGAATTATGACATTATTTATAAATTTAAAGAAGATTTAGAATTATGA", "aa": "MAKKNIKQKKDNRIAIDVKKHIKKVDVGVFDGTFVFTSPLSISELAPKLNKSPNEIIMRYFKKGVVYNLNTILDEEQIGELCLEYDLDFKIEKNVNTENLLENIYFDDLEIDLVARAPIVTIMGHVDHGKTTLLDTIRKSSITASEAGGITQHIGAYQIIKDNRAITFIDTPGHEAFTEMRARGANLTDIVILVVAADDGIKMQTEEAIDHAKAANVPIIVFVNKMDKYEANPEKVLNQLSAKEIVAEELGGDVVFVKGSALKNEGISELLDSILLIAELNNYKANPNRLAYGTTIEANLDKGHGPLATLLVQNGTLRKGDYLVVGSTYGKIRNMFDEYDNEIEIALPSKPVKVSGFEEVPTAGDKFLALADEKQARAIANDVKQKKMRLERAMLQSSDIRTKIANGELKNINLIIKADVQGSLEALKGIFSSINIEGVTTTLIRSAIGTISESDVRLAQTSDAIIIGFNVRASRIIKDLADSVGVQIMNYDIIYKFKEDLEL", "aa_hexdigest": "c05a49accf48ea7381c1682643a79e02", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_Q9PQH1", "query_cov": 1.0, "subject_cov": 0.8192182410423453, "identity": 1.0, "score": 954.0, "evalue": 0.0, "valid": true, "gene": "infB", "product": "translation initiation factor IF-2", "uniref50_id": "UniRef50_Q9PQH1", "cog_id": "COG0532", "cog_category": "J", "kegg_orthology_id": "K02519", "go_ids": [ "GO:0003743", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0005829", "GO:0006413" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQH1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQH1" ], "product": "Translation initiation factor IF-2" }, "genes": [ "infB" ], "id": "NKIECMJPJD_966", "locus": "GCA000828735_00953" }, { "type": "cds", "sequence": "contig_1", "start": 560028, "stop": 560279, "strand": "-", "frame": 1, "gene": "ylxR", "product": "YlxR domain-containing protein", "db_xrefs": [ "RefSeq:WP_006688432.1", "SO:0001217", "UniParc:UPI00000C1C18", "UniRef:UniRef100_A0AAC9X6P2", "UniRef:UniRef50_A0AAC9X6P2", "UniRef:UniRef90_A0AAC9X6P2" ], "nt": "ATGGCAATGAAAGTTAATGAAAGAACTTGTGTTTTTAGTAAAGAAAAATATCCTAAAGATCATTTAGTTCGCTTTGTTATTATTAACGGTGAGTTAATTTTTAGTTTGAATTATCATCGTGGGTATTATTTAATGATTCAAAAGAATACAAACATTCAAAAGGTGTGTCAATTTCTAAAAAAACGTTTTATGATTAAAAACGAAGAACAAGTTTATCGGATTTTAGAGCAATTAATTCAATCATTAATTTAA", "aa": "MAMKVNERTCVFSKEKYPKDHLVRFVIINGELIFSLNYHRGYYLMIQKNTNIQKVCQFLKKRFMIKNEEQVYRILEQLIQSLI", "aa_hexdigest": "05789dfa2d41348fecc04d807132c00a", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00000C1C18", "ncbi_nrp_id": "WP_006688432.1", "uniref100_id": "UniRef100_A0AAC9X6P2", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1C18", "RefSeq:WP_006688432.1", "UniRef:UniRef100_A0AAC9X6P2" ] }, "ips": { "uniref100_id": "UniRef100_A0AAC9X6P2", "uniref90_id": "UniRef90_A0AAC9X6P2" }, "psc": { "uniref90_id": "UniRef90_A0AAC9X6P2", "product": "YlxR domain-containing protein", "uniref50_id": "UniRef50_A0AAC9X6P2" }, "pscc": { "uniref50_id": "UniRef50_A0AAC9X6P2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9X6P2" ], "product": "YlxR domain-containing protein" }, "genes": [ "ylxR" ], "id": "NKIECMJPJD_967", "locus": "GCA000828735_00954" }, { "type": "cds", "sequence": "contig_1", "start": 560290, "stop": 560460, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAGCTTTAATTGATAGTATAGAAGATGATAAAATTGAACAAGATGTCCAATTAAATCACCCATTAAAACAACAAGAAAAAAAAGTTGTTTCTTCAAATGATGAAGATGGTGAAAATGATGAAACACAATTAGAATATCTTGAAGGATTCGAAGATTTTAAATTCTAA", "aa": "MLALIDSIEDDKIEQDVQLNHPLKQQEKKVVSSNDEDGENDETQLEYLEGFEDFKF", "aa_hexdigest": "7b912fa161d2d0e720f1de2e653d9474", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6558.0286, "isoelectric_point": 4.0500284194946286 }, "id": "NKIECMJPJD_968", "locus": "GCA000828735_00955" }, { "type": "cds", "sequence": "contig_1", "start": 561130, "stop": 561222, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAATGATTCGTGCAAAAGTTGCAGAAGTTAATTATAACAAACAACGTAATGAAATTACTGGAGTAAAAGTTGAACTAGATGACCAATGA", "aa": "MKMIRAKVAEVNYNKQRNEITGVKVELDDQ", "aa_hexdigest": "4c024f2fd3a20a93f8704a1cae5ebe31", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3521.0319, "isoelectric_point": 8.139987754821778 }, "id": "NKIECMJPJD_969", "locus": "GCA000828735_00956" }, { "type": "cds", "sequence": "contig_1", "start": 561235, "stop": 561666, "strand": "-", "frame": 3, "gene": "nusA", "product": "NusA N-terminal domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00291363D4", "UniRef:UniRef90_UPI00291363D4" ], "nt": "ATGTCTAACTCATTTAAATCAAAAGAATTTATTGAATACTTTAAAGATACAGCAAAACAAAATGAAATTGAATTTGAAGTTTTATCTTCAATTATTAAAGAAGCATTTGAAAAAGCTTATTCACGAACACATCCTGGTGAAAATTTTGAAACAAATATTGATCTTAAACAAGGAACAATTAATTGTTTTCGAAATTTAGTGGTCGTTGAGAATGAAAAAGTACATAATGAAGATTTAGAAACATGTTTGGATGATGCTGTTGAAATTTTATTAGATGATGCTCGTAAAATCAATGCAAATGCTCAAATTGGCGATCTTATTAAACAACATATTTCAATTGATAATTTTAAAAGTATTGAAGTAGGTCAAATTGGAAGTTTATTGCGTCAAAAAATTACTGAAATTCATAATAAAAGAGTAGCTGATTTTTGA", "aa": "MSNSFKSKEFIEYFKDTAKQNEIEFEVLSSIIKEAFEKAYSRTHPGENFETNIDLKQGTINCFRNLVVVENEKVHNEDLETCLDDAVEILLDDARKINANAQIGDLIKQHISIDNFKSIEVGQIGSLLRQKITEIHNKRVADF", "aa_hexdigest": "d098f2ce75d721084ca9a4ea3d5ac0db", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI00291363D4", "query_cov": 0.8531468531468531, "subject_cov": 0.8356164383561644, "identity": 0.992, "score": 236.0, "evalue": 6.7e-78, "valid": true, "product": "NusA N-terminal domain-containing protein", "uniref50_id": "UniRef50_UPI00291363D4" }, "pscc": { "uniref50_id": "UniRef50_UPI00291363D4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00291363D4" ], "product": "NusA N-terminal domain-containing protein" }, "genes": [ "nusA" ], "id": "NKIECMJPJD_970", "locus": "GCA000828735_00957" }, { "type": "cds", "sequence": "contig_1", "start": 561890, "stop": 562159, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATCTTTTTAATATTATTTATTATTATATTAGTACTTGTTTTAATTTCTATTAATTCATTGTCTGATACATTAAAAGTCGCTAAATTACTAAAAAATATTGGTTATAGTGATTTAAAGAATGCTAAGTTATTTTTAATGGCTTTTTTACCATCATTAATAATTGGATCAATTATTTCAATTCCACTAGTTATTGTAGTAATGCAAGTATTTAGCGAAATAATATTTAATAGTTTAAATGTTTTATTAACAACTAGCTTTATTTGGTGA", "aa": "MIFLILFIIILVLVLISINSLSDTLKVAKLLKNIGYSDLKNAKLFLMAFLPSLIIGSIISIPLVIVVMQVFSEIIFNSLNVLLTTSFIW", "aa_hexdigest": "9d44ae97767b19be74f2c33f6f205ec0", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9939.210899999993, "isoelectric_point": 9.303642082214353 }, "id": "NKIECMJPJD_971", "locus": "GCA000828735_00958" }, { "type": "cds", "sequence": "contig_1", "start": 562526, "stop": 562945, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCATTTATTATCCAATAAAAAACAGATTAATAATTTATTATTTAATAAAAATGATGAGCAAGTAGCAATTATTAATCGAGTAGCTGCCAAATATTACAATTTAAAAATTGGTGATCATTTTAGTTTTGTTGCTAATAATCAAACAAATCGTTATACCAAAGATTACATAAAAAAACAAATCACTTTAAAAGTAGTTGGTATAATTGATACTTATCAGGGGATCGAAATTTATACAGGGATTAAAAATGCCGCTGAAATTTTAGGAATGCATAGTTCGCCATATGCTGATAAATCACTTTATGATGAATACGATGGTGCTTTCAATGGAATTTTTACAAATAAAAATAATCCAGCAATGTTAACACGTATTATTTCTATTTACTCACCTTCGGGACTATATCCTATTTCTGAAACATGA", "aa": "MHLLSNKKQINNLLFNKNDEQVAIINRVAAKYYNLKIGDHFSFVANNQTNRYTKDYIKKQITLKVVGIIDTYQGIEIYTGIKNAAEILGMHSSPYADKSLYDEYDGAFNGIFTNKNNPAMLTRIISIYSPSGLYPISET", "aa_hexdigest": "c7b6345620339e49f1316c49dd8f7e24", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15791.796299999989, "isoelectric_point": 8.981364631652834 }, "id": "NKIECMJPJD_972", "locus": "GCA000828735_00959" }, { "type": "cds", "sequence": "contig_1", "start": 563036, "stop": 563614, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTTATGATGAAGTTTATTTTGAACGTAATAGCAAACAAAATTATCATAAACTAACTTATCAAACTTTTAGTTCTTATTTTAGAGCACGTTTTTTAAAATTTGATCCAGAAGGGGATTTTGATTATTATTTACCATTAAAATATTTTAATGCAACAATTGTTAGAAAAGAATTATTAGAGAATGCAATTAATAATGAAATTCTTTTTAAAAATGACGAAAATAAAATCATTAAAAAAATTAATTTTAAAAGTGATAATGATATAGTTGTTACTAAAATCAATGATTTAACTTATCAAGTTAAAAATATTATTAATCAACAGATTTATCTTTATTCTCCAAAACCCATTAAACGTTCTTATAGAATTGATCCAGCAAAAGCTACAAATGCTATTAAAACAGGATTATCTATTAAATTGGATAATGATTTTATTAATTTAGTATTATTAACTCATCCATCAATTAATCCATCGTATGCAGATGTTTGGTCAGGTATCACATTTAATATGGTAGGTTTTGATTATTTAACAAAAAATGATAAAACACAGTTAAACGAATTTGATGAACCTTATATTTGA", "aa": "MIYDEVYFERNSKQNYHKLTYQTFSSYFRARFLKFDPEGDFDYYLPLKYFNATIVRKELLENAINNEILFKNDENKIIKKINFKSDNDIVVTKINDLTYQVKNIINQQIYLYSPKPIKRSYRIDPAKATNAIKTGLSIKLDNDFINLVLLTHPSINPSYADVWSGITFNMVGFDYLTKNDKTQLNEFDEPYI", "aa_hexdigest": "292774c0d9e68b64d23f1d7caf7b57a3", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 22765.614900000008, "isoelectric_point": 7.751781272888183 }, "id": "NKIECMJPJD_973", "locus": "GCA000828735_00960" }, { "type": "cds", "sequence": "contig_1", "start": 564086, "stop": 564637, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCGTGGAACTGAAACATTTAAAATTTCTAAAATTGTTTATGTAATTAAATTTCCATTTAAATATATTAGTGGTTTTAATAAGTATTTAATTACACTATCTTTTTCATCAATTACAAGAATTATTATTTTTTCAATTATGATTGCTTTATCATTTGGATCGATTATGTTTTTAACTGGAACAAGAGGTAAAATTAAACAAACTATAGATGCAACATTACAAACAAAAAAATATCGTTATGGAATTGATTTAATAACACCAACAGATCAAGGTGGTCAATATATTTCTTTAAGTACAAATCAAATTGGTTCTACATTAAATGATAATGATAATCATGAATTGACACAGAAATATGAAAGTAGTGATTATAAAGATTTTATTAATTCACCGTTATTTAAAAATTTACAGAATTTAAAGTTAATTGGTGCAAAAGATGACCAGCGTCGAATTTATGATTTAAACTATATTGCTAATGCCTTTCAAGTTAAGCAATTTTTAGATTTTAATTTTGGCATTGGTGATGTTAATAGTGGTAGTTCAAGTAATCCATGA", "aa": "MRGTETFKISKIVYVIKFPFKYISGFNKYLITLSFSSITRIIIFSIMIALSFGSIMFLTGTRGKIKQTIDATLQTKKYRYGIDLITPTDQGGQYISLSTNQIGSTLNDNDNHELTQKYESSDYKDFINSPLFKNLQNLKLIGAKDDQRRIYDLNYIANAFQVKQFLDFNFGIGDVNSGSSSNP", "aa_hexdigest": "f498af710d3052de500991a5294bee9e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 20798.499099999986, "isoelectric_point": 9.396798896789551 }, "id": "NKIECMJPJD_974", "locus": "GCA000828735_00961" }, { "type": "cds", "sequence": "contig_1", "start": 564882, "stop": 565082, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTTTTCAACGAGGAACACCATTAGCAATAGAAATACCAATATTAATTTTATTTTGTGCTAAAAAACGGTTGATGATGATAACAATTACAAAAATTGTTAGAATCAAAATAAATAAAGTTATTAAAACACTAAAAGTTTGTATTGTGTTCGTAATTTTGTTAATGAAAACTAATCTTAATGGGCTTGGTGTAATATGA", "aa": "MIFQRGTPLAIEIPILILFCAKKRLMMITITKIVRIKINKVIKTLKVCIVFVILLMKTNLNGLGVI", "aa_hexdigest": "98d4db916aef814cf9cb89c9195637a6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7494.563399999998, "isoelectric_point": 10.78035488128662 }, "id": "NKIECMJPJD_975", "locus": "GCA000828735_00962" }, { "type": "cds", "sequence": "contig_1", "start": 565127, "stop": 565438, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGATGAAAAATATATTATTCATATTGATAACACACCTTATTTAATTTTAGATAGTGGTATTACTCCTGATTTTATGTATCCGATTATCTCATTCACTTCTGTTATTCCTAATCCAAAAAAAGAAGGACTAGTATATGTTAACTTAAATGGATATGCCCGTATTTACGATGCTTTTAGATCTAATCCAATTGAAAATAATTTACAAATTCGTATCACAAATCAAAAGCATCAATCCGTTGATATCAGTATTTTAAAAAAAATTGTTAAAAATTTAAATGATCAAATTAAAAAAGATGCTATCATGAACTGA", "aa": "MDEKYIIHIDNTPYLILDSGITPDFMYPIISFTSVIPNPKKEGLVYVNLNGYARIYDAFRSNPIENNLQIRITNQKHQSVDISILKKIVKNLNDQIKKDAIMN", "aa_hexdigest": "627b7564679e7924bdf52dd3ae63ebb3", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11909.632499999987, "isoelectric_point": 7.934591484069824 }, "id": "NKIECMJPJD_976", "locus": "GCA000828735_00963" }, { "type": "cds", "sequence": "contig_1", "start": 565496, "stop": 565696, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTATATCCATCAAAAAAAGGTATAAGTATAAATGGTAATGTTAATACTATTGTTAAAGGGCATCGAATAACTTTTAGTTTTGAACCCGCAGGGATTCCTATTCCAGTCTATTTTGATGTTAAATCTGCATACGCATTAGTTGGTTTAGGTGCTTTTTTGTCTAATCATAACAAACAAAAATACCCAATGTATTTATGA", "aa": "MLYPSKKGISINGNVNTIVKGHRITFSFEPAGIPIPVYFDVKSAYALVGLGAFLSNHNKQKYPMYL", "aa_hexdigest": "2211a27bf1f2a44160ced0e4be047f18", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7317.488500000002, "isoelectric_point": 9.756339073181149 }, "id": "NKIECMJPJD_977", "locus": "GCA000828735_00964" }, { "type": "cds", "sequence": "contig_1", "start": 565856, "stop": 566197, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTGTTAATTCGACAAAAGATGATATTACTTATAAAATTGTTCAGTATTCAACAAGTAAAACGATTAATACATTCGATAAACAAACAAGTGAACGTTTGATTAAAAATAGTTTTTCTGATATTGATTTATATGGGATTTTAAATAATGCTAAAGTAGATGTTATTGATTTAATCAAAACTAAAACTTTAAATGCCAGTTTTGTCATTAATAATTTAAAAGAATTAGACAATAAACAAAAAAGTAATTTGGTAAAATTAGCTAAAGTTGATAATTACGATGTTAGTATTAGTCAACAAGCACGACTTTATTTATTAAAAAATATTGTTTATGGTGCATGA", "aa": "MIVNSTKDDITYKIVQYSTSKTINTFDKQTSERLIKNSFSDIDLYGILNNAKVDVIDLIKTKTLNASFVINNLKELDNKQKSNLVKLAKVDNYDVSISQQARLYLLKNIVYGA", "aa_hexdigest": "b80b313ba73e87508e3221032816c22f", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12852.592399999983, "isoelectric_point": 9.247038841247559 }, "id": "NKIECMJPJD_978", "locus": "GCA000828735_00965" }, { "type": "cds", "sequence": "contig_1", "start": 566213, "stop": 566581, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAAGTATAATTTTAAACAATGAAAACAAAAAAAGAGAAGATAAAAACATTTTTTTGCTTAAAATTATTATTATGTTTAGAAATTTAATTAAGAATGTCTATCGTTATTTGCTAAAAAGCAAAGCCTCTTTTATAGGTCTTTGCCTTTTATTGTTTGTGTCAATCTTTAGTTTTAGTATGTTAACAAATCTTTCTAAAACATTAACAAGATCATATACAAACTTAGTCAATAGACAAAAATTACATGATATAACTGTTAATGAAAGTTATATTGAAGATCAAAAAGATATTAAACAAGCTCAATTCAATAAGCTTTTAGAAGAATTAAAAGTTTTTTATGCACAAAAAGGTTTGAATTTTGATTGA", "aa": "MKSIILNNENKKREDKNIFLLKIIIMFRNLIKNVYRYLLKSKASFIGLCLLLFVSIFSFSMLTNLSKTLTRSYTNLVNRQKLHDITVNESYIEDQKDIKQAQFNKLLEELKVFYAQKGLNFD", "aa_hexdigest": "14944b4233addfb9d0a4d1d88edbd772", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14475.918999999978, "isoelectric_point": 9.743251991271972 }, "id": "NKIECMJPJD_979", "locus": "GCA000828735_00966" }, { "type": "cds", "sequence": "contig_1", "start": 566931, "stop": 567095, "strand": "-", "frame": 1, "gene": null, "product": "DUF3627 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T0B9", "UniRef:UniRef90_A0AAC9T0B9" ], "nt": "ATGAAAAATTTTTCAAAAAATAGAAATATTAATAATTTTAAAAACCCTTTAAGAGTTATTGAAGGAATTGTTCGTGAAATTAAAAATAATGAACTTCAAATTGATTTTGTTAATAGTCATAAAAAAGGAGTTTGTAAGTTTATTAATTTGACAGATTATCATTGA", "aa": "MKNFSKNRNINNFKNPLRVIEGIVREIKNNELQIDFVNSHKKGVCKFINLTDYH", "aa_hexdigest": "d36d07e1c8d0cba4f851955e7db45ee0", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 566715, "stop": 567095, "observations": { "mutation": [ 566849, 566933 ], "stop": [ 566849, 566933 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAC9T0B9", "query_cov": 1.0, "subject_cov": 0.42857142857142855, "identity": 1.0, "score": 111.0, "evalue": 2.06e-30, "gene_start": 1, "gene_end": 54, "reference_start": 1, "reference_end": 54, "reference_sequence": "MKNFSKNRNINNFKNPLRVIEGIVREIKNNELQIDFVNSHKKGVCKFINLTDYHWNTISSRFLINSKHLFLVSKFDPIRHVYWLNYKIIHPIEIKNKRRSYPTLSHDRNLRIFLNNLLEKENELKN" }, "paralog": false, "identity": 0.9841269841269841, "score": 251.0, "evalue": 2.53e-88, "description": "Internal stop codon at 566849, 566933. Nonsense mutation around 566849, 566933.", "psc": { "uniref90_id": "UniRef90_A0AAC9T0B9", "product": "DUF3627 domain-containing protein", "uniref50_id": "UniRef50_A0AAC9T0B9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAC9T0B9", "UniRef:UniRef50_A0AAC9T0B9" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAC9T0B9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T0B9" ], "product": "DUF3627 domain-containing protein" } }, "id": "NKIECMJPJD_980", "locus": "GCA000828735_00967" }, { "type": "cds", "sequence": "contig_1", "start": 567261, "stop": 567605, "strand": "+", "frame": 3, "gene": null, "product": "inorganic diphosphatase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021B06C44" ], "nt": "ATGAAATTAAATGTAACTATTGAAATTCCAAAGAACTCAAATATTAAATATGAATATGATCGTGCAACTAAAGAAATCATGGTTGATCGTATCTTACACGGTTCAATGGTTTATCCTCATAATTATGGTTTTTTAAAAGAAGCATTAGATTATGATGGTGACGAATTGGATGTTTTGGTTTTTGCTGATCAAGCATTCCAACCAGGAATTAAAGTACCTGCACGTGTTTTGGGCGCCATGAAAATGATTGATGGAGGAGAAACAGATACTAAATTATTGGCAGTTATTGATGTTGATCCTCGTTATAAACACATTAATAATTTCAAAGATATTCCATTACATTGA", "aa": "MKLNVTIEIPKNSNIKYEYDRATKEIMVDRILHGSMVYPHNYGFLKEALDYDGDELDVLVFADQAFQPGIKVPARVLGAMKMIDGGETDTKLLAVIDVDPRYKHINNFKDIPLH", "aa_hexdigest": "c64f7c44daa3219150d1c25282f87cde", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_UPI0021B06C44", "query_cov": 0.9824561403508771, "subject_cov": 0.8549618320610687, "identity": 0.509, "score": 103.0, "evalue": 2.05e-26, "valid": false, "product": "inorganic diphosphatase", "uniref50_id": "UniRef50_UPI0021B06C44" }, "pscc": { "uniref50_id": "UniRef50_UPI0021B06C44", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021B06C44" ], "product": "inorganic diphosphatase" }, "genes": [], "id": "NKIECMJPJD_981", "locus": "GCA000828735_00968" }, { "type": "cds", "sequence": "contig_1", "start": 567806, "stop": 568033, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTCAAAAAATCAAAAATTCAAATTTTTATATAATAATTAAAAAATTTAATCAATCACTTAGAAAAATAAATTTTTTTGAGAAAATTATTTTAAAAATAAACAATAAAAGCATTCGTGATTACTTTTTATCAATAACATCATTAGATGTAATTTTTTATAAAATAATTAATTTTATTATTGTTGCTTTAATTGTGCTATTATCAGGTTTTTTGGTTCGTGAATGA", "aa": "MLQKIKNSNFYIIIKKFNQSLRKINFFEKIILKINNKSIRDYFLSITSLDVIFYKIINFIIVALIVLLSGFLVRE", "aa_hexdigest": "ae729c869640f11e0e2caabe2687866a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8958.769399999996, "isoelectric_point": 10.140183830261229 }, "id": "NKIECMJPJD_982", "locus": "GCA000828735_00969" }, { "type": "cds", "sequence": "contig_1", "start": 568223, "stop": 568384, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTTTTTCTATTATTTGGCGGTTTAGGAAATATTATTGATCGCTCAATTCCAGAATTTGAATTATTCAATGCTTTACCAAATATATTTCACAATAGCCTTATAAATCCTAATAGTGTTGGTGGAGGCGATGATGTTATTAATGGTGTTGTTGATTATTGA", "aa": "MLFLLFGGLGNIIDRSIPEFELFNALPNIFHNSLINPNSVGGGDDVINGVVDY", "aa_hexdigest": "1bba94d8d9df5ebb72ab8efc60b7fd94", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5749.461600000002, "isoelectric_point": 4.0500284194946286 }, "id": "NKIECMJPJD_983", "locus": "GCA000828735_00970" }, { "type": "cds", "sequence": "contig_1", "start": 568621, "stop": 569499, "strand": "+", "frame": 1, "gene": null, "product": "Pseudouridine synthase", "db_xrefs": [ "EC:5.4.99.23", "KEGG:K06180", "SO:0001217", "UniParc:UPI000165F8BB", "UniRef:UniRef100_Q9PQH8", "UniRef:UniRef50_Q9PQH8", "UniRef:UniRef90_Q9PQH8" ], "nt": "ATGTCGTCTCAACGCATCGATAAATTTTTAGTAGAAAATACTAATTATTCACGAACATTAATTAAAAAAATGTTAGACCAAAACCTAATATTAGTTAATGACAATTTTATTAGTAAAGCAAGTTATTTATTAAAAAAGAATGATTATATTCAAATCAATAACGAACTACCTATAGTGCAAGAGCAAAAAAAATTGTTACCGTTTGTATATTCATTAGAAATTATCTTTGAAGATCAGGATCTATTGATCGTTAACAAACCTTCTGGTATGTTAGTTCATCCAACTAGTTTTCATGAGCAAAATACATTAGCAAACGCTTTAAAATATTATTTAAAAACTGATAATTTTTATATTACACATCGTTTGGATAAAAATACATCTGGATTAATTATAATTTGTAAAAATCAAATAGCGCTTAATTCTTTGCAACAACAATTTATGCAAAAAACCATAAGAAAAAGTTATTATGCTATTGTTCATAATCGTTTTGATATGCAGCATTTGCATTTTATGATCAATGAGCCTATTGGCCATAGTTATCAAGATAAATTAAGAATGAAAACAGGCAATTCTAAAAATACTAAAGAGGCTATCAGTATCGTTAAAGTACTTGAGCAATATAAAAATACCGCCCTTGTTGAAGTAAGTATTATTACTGGTAGAACTCATCAAATACGGGTTCATATGCGTTATATTAATCACCCTGTTTATAATGATCCTTTATATGGGATTAGTAAACATACCACAGAATACGAGCAATACTTGCATAGTTTTTATATTAGTTTTAATCATCCATCCACTAATAAAAAAGTAGAATTTAAAATCAATATGCCAAAAGAATTTATGGAATTAATTAGAGAGTTAAAAGCAAATGAATAG", "aa": "MSSQRIDKFLVENTNYSRTLIKKMLDQNLILVNDNFISKASYLLKKNDYIQINNELPIVQEQKKLLPFVYSLEIIFEDQDLLIVNKPSGMLVHPTSFHEQNTLANALKYYLKTDNFYITHRLDKNTSGLIIICKNQIALNSLQQQFMQKTIRKSYYAIVHNRFDMQHLHFMINEPIGHSYQDKLRMKTGNSKNTKEAISIVKVLEQYKNTALVEVSIITGRTHQIRVHMRYINHPVYNDPLYGISKHTTEYEQYLHSFYISFNHPSTNKKVEFKINMPKEFMELIRELKANE", "aa_hexdigest": "0b6d746320ae0bbfb5397df7a095b08b", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000165F8BB", "uniref100_id": "UniRef100_Q9PQH8", "db_xrefs": [ "SO:0001217", "UniParc:UPI000165F8BB", "UniRef:UniRef100_Q9PQH8" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQH8", "uniref90_id": "UniRef90_Q9PQH8" }, "psc": { "uniref90_id": "UniRef90_Q9PQH8", "product": "Pseudouridine synthase", "ec_ids": [ "5.4.99.23" ], "uniref50_id": "UniRef50_Q9PQH8", "kegg_orthology_id": "K06180" }, "pscc": { "uniref50_id": "UniRef50_Q9PQH8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQH8" ], "product": "Pseudouridine synthase" }, "genes": [], "id": "NKIECMJPJD_984", "locus": "GCA000828735_00971" }, { "type": "cds", "sequence": "contig_1", "start": 569492, "stop": 570067, "strand": "+", "frame": 2, "gene": "tsaB", "product": "tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex dimerization subunit type 1 TsaB", "db_xrefs": [ "COG:COG1214", "COG:J", "EC:2.3.1.234", "GO:0002949", "RefSeq:WP_006689124.1", "SO:0001217", "UniParc:UPI00000C1C14", "UniRef:UniRef100_A0AAC9X7X4", "UniRef:UniRef50_A0AAC9X7X4", "UniRef:UniRef90_A0AAC9X7X4" ], "nt": "ATGAATAGTACATACCAATTGTTTATTGATGTTACATCAAGAAAATGTATTTTAGCAATTTATAAAAACTTTAAAATCCATACTAGCATTATTGTTGATACAAACAATAATCTTACAGATATTATTGTTGAACACCTTAATAATTTATTAAAAATCACTAACTTGCAATATCAAGATCTAGAAGCAATATATTTAAATACTGGTCCAGGATCATTTACTGGAATACGTGTTGGTGCTATTGTTGCTAAAACGATTTGTACAATTTTTAATAAAATTAAACTTTTTATTAATGATAGTTTAAATATTATAGCCAATGGTAAGAATGATGTTTTTGTTTATTTAGATGCTAAAGGTAATAAATCGTATGTAATAACTATTACTAATAATATTCAAAACGAATATAAAATTATTACTAATAAAGAATTACAAAATGAATTAAAAAATACATCATTAACAATAATTGATGCAAATCAAATTGACTATCATGATCTCATATATAACTTAAAATTTGATAATTTTAAGTTAACTAATATCTTAGATTTTAGATTAAATTATGTTAAAAAACCTCTTAATTAG", "aa": "MNSTYQLFIDVTSRKCILAIYKNFKIHTSIIVDTNNNLTDIIVEHLNNLLKITNLQYQDLEAIYLNTGPGSFTGIRVGAIVAKTICTIFNKIKLFINDSLNIIANGKNDVFVYLDAKGNKSYVITITNNIQNEYKIITNKELQNELKNTSLTIIDANQIDYHDLIYNLKFDNFKLTNILDFRLNYVKKPLN", "aa_hexdigest": "34fecd2a37d32594dddd2b7bb90ca4db", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI00000C1C14", "ncbi_nrp_id": "WP_006689124.1", "uniref100_id": "UniRef100_A0AAC9X7X4", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1C14", "RefSeq:WP_006689124.1", "UniRef:UniRef100_A0AAC9X7X4" ] }, "ips": { "uniref100_id": "UniRef100_A0AAC9X7X4", "uniref90_id": "UniRef90_A0AAC9X7X4" }, "psc": { "uniref90_id": "UniRef90_A0AAC9X7X4", "gene": "tsaB", "product": "tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex dimerization subunit type 1 TsaB", "ec_ids": [ "2.3.1.234" ], "uniref50_id": "UniRef50_A0AAC9X7X4", "cog_id": "COG1214", "cog_category": "J", "go_ids": [ "GO:0002949" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAC9X7X4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9X7X4" ], "product": "tRNA (Adenosine(37)-N6)-threonylcarbamoyltransferase complex dimerization subunit type 1 TsaB" }, "genes": [ "tsaB" ], "id": "NKIECMJPJD_985", "locus": "GCA000828735_00972" }, { "type": "cds", "sequence": "contig_1", "start": 570045, "stop": 570467, "strand": "+", "frame": 3, "gene": "rimI", "product": "N-acetyltransferase", "db_xrefs": [ "COG:COG0456", "COG:J", "SO:0001217", "UniRef:UniRef50_A0AAC9X727", "UniRef:UniRef90_A0AAC9X727" ], "nt": "ATGTTAAAAAACCTCTTAATTAGACCTGCTGATCAAAAAGATTTGAATGATATTGCTATTTTTGAAAATAGATTTTTCTTAAATGATTGTTATTCGATGAGAAATTTAGAAGAAATTTTTCATAATTCAAATTATCAAATAATAATTGTTGAATATGAGCAAAAAAACATTGCTTATTTAATTTATATGAAGAGTGTTGATTTTAATGAAATTTTAAAAATTGGTGTTGATGAAAATTATCGTAATTTAAAAATTGGTAGTTTATTAATTGATTTATTAAAACAAAAAAAGCAAAACATATTATTAGAAGTAAGCGATCTTAATACAAATGCATTAAATTTTTATTTAAAGCATGGTTTTATAAAACAAAACATTCGCAAAAGATATTATGCCAATGGTTCTGATGCTATTTTAATGATTTGA", "aa": "MLKNLLIRPADQKDLNDIAIFENRFFLNDCYSMRNLEEIFHNSNYQIIIVEYEQKNIAYLIYMKSVDFNEILKIGVDENYRNLKIGSLLIDLLKQKKQNILLEVSDLNTNALNFYLKHGFIKQNIRKRYYANGSDAILMI", "aa_hexdigest": "1bbc0d30d0733eb8c18d23fa86f72ae7", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0AAC9X727", "query_cov": 1.0, "subject_cov": 0.958904109589041, "identity": 1.0, "score": 278.0, "evalue": 1.42e-94, "valid": true, "gene": "RimI", "product": "N-acetyltransferase", "uniref50_id": "UniRef50_A0AAC9X727", "cog_id": "COG0456", "cog_category": "J" }, "pscc": { "uniref50_id": "UniRef50_A0AAC9X727", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9X727" ], "product": "N-acetyltransferase" }, "genes": [ "rimI" ], "id": "NKIECMJPJD_986", "locus": "GCA000828735_00973" }, { "type": "ncRNA", "class": [ "RNase_P_RNA", "SO:0000386" ], "sequence": "contig_1", "start": 570529, "stop": 570891, "strand": "-", "gene": "RNaseP_bact_b", "product": "Bacterial RNase P class B", "score": 243.3, "evalue": 2.2e-26, "db_xrefs": [ "RFAM:RF00011", "SO:0000386" ], "nt": "GTAAAAAGCTAATTGCTGCATTTTTTAAAAAATGTAGAGGAAAGTCCGTGCTCACACTAACTGTGATGTTAGTAGTGTTTGTGTATGTATAATTAATAAGGCATAGCATAGGCATATGACGGCAAATTTTTAAGCTAAGTGATTTAAATTATATGTTTAAAAATTTATAAAAGTGCCACAGAGACGATTAATCAAGAAATTGATGAGATGAAACGTTGGTAAACCCCACAAGTGAGAAACCTAAATTTCGGTAAGGGAATCTAATTTAGTAAATTAAGAAATTAAATTAGGATAGAATTGTTTACATTCTATAGATAAATAATTAGCGCTACATTTTTGTAGAACAGAACACGGCTTATTTTT", "id": "NKIECMJPJD_38", "locus": "GCA000828735_00974" }, { "type": "cds", "sequence": "contig_1", "start": 571074, "stop": 571328, "strand": "+", "frame": 3, "gene": "rpsT", "product": "30S ribosomal protein S20", "db_xrefs": [ "COG:COG0268", "COG:J", "GO:0003723", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0015935", "GO:0070181", "KEGG:K02968", "RefSeq:WP_006688746.1", "SO:0001217", "UniParc:UPI0000134E5E", "UniRef:UniRef100_Q9PQI1", "UniRef:UniRef50_Q9PQI1", "UniRef:UniRef90_Q9PQI1" ], "nt": "ATGGCAAATATTGTTTCAAATGAAAAAACATACAGACATACGCAAAAAGTACGTAAGGAAAATCATGCTAAAATGTCTAAATTAAGAACAATTGTTAAAAAAACAAGAAGTTCAAATGAACAAGCTCAACTTAATGAAGCTTACAAAGTCATTGATACAACAGCTTCAAAAGGTGTTATTCACAAAAATAAAGCAAATCGTTTAAAATCAAGAACAGCAAAAGCTTTTAAAACAAACTTAGAAGTCACAGCATAA", "aa": "MANIVSNEKTYRHTQKVRKENHAKMSKLRTIVKKTRSSNEQAQLNEAYKVIDTTASKGVIHKNKANRLKSRTAKAFKTNLEVTA", "aa_hexdigest": "8a65af6ef039e1d1e11a57ddf0b133fa", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0000134E5E", "ncbi_nrp_id": "WP_006688746.1", "uniref100_id": "UniRef100_Q9PQI1", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000134E5E", "RefSeq:WP_006688746.1", "UniRef:UniRef100_Q9PQI1" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQI1", "uniref90_id": "UniRef90_Q9PQI1" }, "psc": { "uniref90_id": "UniRef90_Q9PQI1", "gene": "rpsT", "product": "30S ribosomal protein S20", "uniref50_id": "UniRef50_Q9PQI1", "cog_id": "COG0268", "cog_category": "J", "kegg_orthology_id": "K02968", "go_ids": [ "GO:0003723", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0015935", "GO:0070181" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQI1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQI1" ], "product": "Small ribosomal subunit protein bS20" }, "genes": [ "rpsT" ], "id": "NKIECMJPJD_987", "locus": "GCA000828735_00975" }, { "type": "cds", "sequence": "contig_1", "start": 571396, "stop": 571851, "strand": "-", "frame": 3, "gene": null, "product": "Sigma-70 family RNA polymerase sigma factor", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9X6I6", "UniRef:UniRef90_A0AAC9X6I6" ], "nt": "TTGAATTTTAAATTTTATACTTTAGAGATTGAAGCAAATGATTTAAAAACTATTATTTATGAAATATTTTTAAAAATATTTCAAGTAACTAGAATTAAAAGTTTAAATCAATTTATAGGATTTGTTAAACGGCAAATTTATTTTTATTTAGTATATTTATTACGTAAATCTTTATCTAATAAAAATTTAGTTAATCATTTAAATAATAATAATTTTGAAAATTATGAATTAATTAGTACCAATTCAGATGAAGTTGGTGCTGAATTAAACAACAGATTTTTAATTTATGAATTTTTAAGAAGTATTCAGAAAGAAAATTTATTTTTATATCATTTTACTATTTTAATTATTAAAGGATATAATACAAAGGAAATTAGTGAAATTTTAAAAATTAATTCTGCAAAAATTTATTATTATAAAGCACTTTTAAAAAAATATATAAAAAAATTATTATAA", "aa": "MNFKFYTLEIEANDLKTIIYEIFLKIFQVTRIKSLNQFIGFVKRQIYFYLVYLLRKSLSNKNLVNHLNNNNFENYELISTNSDEVGAELNNRFLIYEFLRSIQKENLFLYHFTILIIKGYNTKEISEILKINSAKIYYYKALLKKYIKKLL", "aa_hexdigest": "93fedae9361fa4c7765900a45c69163b", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0AAC9X6I6", "query_cov": 1.0, "subject_cov": 0.8118279569892473, "identity": 0.993, "score": 280.0, "evalue": 1.52e-94, "valid": true, "product": "Sigma-70 family RNA polymerase sigma factor", "uniref50_id": "UniRef50_A0AAC9X6I6" }, "pscc": { "uniref50_id": "UniRef50_A0AAC9X6I6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9X6I6" ], "product": "Sigma-70 family RNA polymerase sigma factor" }, "genes": [], "id": "NKIECMJPJD_988", "locus": "GCA000828735_00976" }, { "type": "cds", "sequence": "contig_1", "start": 572293, "stop": 572400, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATATAGTAATTAAGAAAAATTGTAATTTTGTTAAAAATGATGACCATTTATATTCTTTTTATACACCACTAATTGGTATGCAAGCAGTAGCTTTATATTCTTGA", "aa": "MDIVIKKNCNFVKNDDHLYSFYTPLIGMQAVALYS", "aa_hexdigest": "038fd05ed8d7fdb8e46125d83ad2e941", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4052.6922000000004, "isoelectric_point": 6.498140525817871 }, "id": "NKIECMJPJD_989", "locus": "GCA000828735_00977" }, { "type": "cds", "sequence": "contig_1", "start": 572887, "stop": 573438, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAAATTTAATCAAAAATAATTTAATTAATAATATTGATTTTTTAACAATTGATTTAAATAAATTAGAATTAACAATTTTTTCAAATATGGAATTCGAAAAAAATAAAACAAAATTTCAATATTACAATATTGAACGTGATTTTAATATTTTTAATAAAAACAGTAATTTAGATCGATTCAAAAATGTAATTAATGATTATAATAATTTTAAACCTTCAGATTTTTTAAAACATATTCGTAAAGAAAAATTAAGTTTAAAAGATAAAGAGGCTATCAATTTATTAACGTATGACAAATCATTAAGTGATCCAATTGTTAATGTTATCTTAGATTTTGTTTTATTTAAAAATTTAGGGCGTTTAAATGTAAAATATTTAAAACGTATTAAAGAAACAGTACTGGGAATCAATATTAAAGATGAGTATGAAATGATTAAATTTTTTCAAAATAAAAAAAATGATCATAAAGAATTAATTTGTGAAGATAGTGATAATGCTAATACTTTCAAAAATACTAATTTTATTGAAGTAAAATTTGGAGATTTATAA", "aa": "MLNLIKNNLINNIDFLTIDLNKLELTIFSNMEFEKNKTKFQYYNIERDFNIFNKNSNLDRFKNVINDYNNFKPSDFLKHIRKEKLSLKDKEAINLLTYDKSLSDPIVNVILDFVLFKNLGRLNVKYLKRIKETVLGINIKDEYEMIKFFQNKKNDHKELICEDSDNANTFKNTNFIEVKFGDL", "aa_hexdigest": "c600f5afd5a08cbc6175c9d3695269f5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 21879.92050000001, "isoelectric_point": 8.75205059051514 }, "id": "NKIECMJPJD_990", "locus": "GCA000828735_00978" }, { "type": "cds", "sequence": "contig_1", "start": 573441, "stop": 573563, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACAATAATCAAGTTGATGATGAATTAGATTTAGAGAATTTTAATTATCAAAAAGCATTAGAAATTCCGAATATTAAAGCAATTAATTTAACAGAAGAAGAATTTAATTTATACTTTTGA", "aa": "MNNNQVDDELDLENFNYQKALEIPNIKAINLTEEEFNLYF", "aa_hexdigest": "f562db6573753fa41b5c8bcf04baee46", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4807.215799999999, "isoelectric_point": 4.0500284194946286 }, "id": "NKIECMJPJD_991", "locus": "GCA000828735_00979" }, { "type": "cds", "sequence": "contig_1", "start": 573591, "stop": 574157, "strand": "+", "frame": 3, "gene": "dnaC", "product": "ATP-binding protein", "db_xrefs": [ "COG:COG1484", "COG:L", "SO:0001217", "UniRef:UniRef50_A0AA86Z0S2", "UniRef:UniRef90_A0AAC9T3C2" ], "nt": "TTGAATAATTTAAACAATCAAAATGATAGTTATTACATTTATCAATTAAATCGTAATGAATATAATCACTTATGTTTAGTAGTAGTTAAAAAAGAAAATAAAGTAGATAAAATTAAAAGAAATTATATTTTAAATACGATCAAAAATATGGATTATAATATCTCATTAGCAGATGATAGTCAAATATTTTCTAAAAAAACTGAAATATTAGATGCTAATTTATTAGCAGAACGAAATAAATTAATTAATTTCTTTTTAGAAGAAGCACGAAAAAATAAAAAAAATACGTTAATTAAAAATAATAATCAACAACCAAAATCTGCTTTTATTTATGGTGATTTTAGTGTAGGCAAATCAATAATTACTCAAGCTTATACCAATACTATTTCATTAAAATATAACTTAAAGATAGCTTATATTACTTTAAATGATCTATTTAAAAATGTAATCCAATTTTTTAACTACAAAGATACATCAGATTTAGTAATTAATGAATTAGTTAATCTACTTTCAAATGTTGATGTTTTAGTAATTGATGATTTTTCTAGCGTTAATTTAAATTACTGA", "aa": "MNNLNNQNDSYYIYQLNRNEYNHLCLVVVKKENKVDKIKRNYILNTIKNMDYNISLADDSQIFSKKTEILDANLLAERNKLINFFLEEARKNKKNTLIKNNNQQPKSAFIYGDFSVGKSIITQAYTNTISLKYNLKIAYITLNDLFKNVIQFFNYKDTSDLVINELVNLLSNVDVLVIDDFSSVNLNY", "aa_hexdigest": "986bc07de6293bfd76cb2985e122cd41", "start_type": "TTG", "rbs_motif": null, "genes": [ "dnaC" ], "truncated": "both", "pseudogene": { "start": 573441, "stop": 574391, "observations": { "mutation": [ 573561, 574155 ], "stop": [ 573561, 574155 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAC9T3C2", "query_cov": 1.0, "subject_cov": 0.5949367088607594, "identity": 0.995, "score": 356.0, "evalue": 2.98e-122, "gene_start": 1, "gene_end": 188, "reference_start": 51, "reference_end": 238, "reference_sequence": "MNNNQVDDELDLENFNYQKALEIPNIKAINLTEEEFNLYFWDIVEVYRSYLNNLNNQNDSYYIYQLNRNEYNHLCLVVVKKENKVDKIKRNYILNTIKNMDYNISLADDSQIFSKKTEILDANLLAERNKLINFFLEEARKNKKNTLIKNNNQQPKSAFIYGDFSVGKSIITQAYTNTISLKYNLKIAYITLNDLFKNVIQFFNYKDTSDLVINELVNLLSNVDVLVIDDFSSVNLNYWSISTILMPIIENRLKSTRQTIFISNFSIEQLINSTKNTTNIEEQKTKLRLFNRIEYLTYGNIFKITGPSVFKVVNNS" }, "paralog": false, "identity": 0.9936708860759493, "score": 614.0, "evalue": 2.13e-226, "description": "Internal stop codon at 573561, 574155. Nonsense mutation around 573561, 574155.", "psc": { "uniref90_id": "UniRef90_A0AAC9T3C2", "gene": "DnaC", "product": "ATP-binding protein", "uniref50_id": "UniRef50_A0AA86Z0S2", "cog_id": "COG1484", "cog_category": "L", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAC9T3C2", "UniRef:UniRef50_A0AA86Z0S2", "COG:COG1484", "COG:L" ] }, "pscc": { "uniref50_id": "UniRef50_A0AA86Z0S2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AA86Z0S2" ], "product": "Chromosomal replication initiator protein DnaA ATPAse domain-containing protein" } }, "id": "NKIECMJPJD_992", "locus": "GCA000828735_00980" }, { "type": "cds", "sequence": "contig_1", "start": 574176, "stop": 574391, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCTATTATTGAAAATAGATTAAAATCAACAAGACAAACCATTTTTATTTCTAATTTTTCAATTGAACAATTAATTAATTCAACAAAAAATACAACAAATATTGAAGAACAAAAAACTAAATTACGCTTATTTAATCGTATCGAATATCTCACATATGGTAATATTTTTAAAATAACAGGGCCAAGTGTTTTTAAAGTTGTAAATAATTCATAA", "aa": "MPIIENRLKSTRQTIFISNFSIEQLINSTKNTTNIEEQKTKLRLFNRIEYLTYGNIFKITGPSVFKVVNNS", "aa_hexdigest": "50c44174bcf7dd58591b2e4841215fb3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8296.469299999995, "isoelectric_point": 9.991777610778808 }, "id": "NKIECMJPJD_993", "locus": "GCA000828735_00981" }, { "type": "cds", "sequence": "contig_1", "start": 574623, "stop": 575543, "strand": "-", "frame": 1, "gene": "nfo", "product": "deoxyribonuclease IV", "db_xrefs": [ "EC:3.1.21.2", "GO:0003677", "GO:0003906", "GO:0006281", "GO:0006284", "GO:0008081", "GO:0008270", "GO:0008833", "SO:0001217", "UniParc:UPI000165F8B3", "UniRef:UniRef100_Q9PQI5", "UniRef:UniRef50_Q9PQI5", "UniRef:UniRef90_Q9PQI5" ], "nt": "ATGAATAATATGGATAAATATAATTTAATTATAGGTAGTCATGTAAGTTTAAAAGCTAAAGATTTCTTTTATGGTAGTGTAAAAGAAGCATTATCTTATGGTTCTAACACGTTTATGGTTTATACTGGAGCTCCACAAAATACAAAACGTCAACCGATTAAATCATTTAAAATTGAAGAAGCACATAATTTGTTAAAAAAACATAATATTAATTTGGATGATTTAATTGTTCACGCTCCATATATTATCAACCCTTGTTCTTCTAAAAAGAATGTTCGTGAACTTGCTAAAGAATTTTTAATTCAGGAAATCCAACGAACAGAATCTATGGGGATCACTAAACTTGTTTTACATCCAGGATCACGTTTAGAACAAAATGAAGATATAGCTTTAGAACAAGTTTATACAATGTTAAATGATATTTTTTCAACTATTAATACTAATGTAATTGTTTGCTTAGAGACCATGGCTGGTAAAGGTTCTGAAATTGGGGTAAATATAAAACAATTAAAAACCATTATAGACAATGTTCATTCTAAAAAAAATATTGGCGTTTGTTTAGATACTTGCCATATGAATGATAGCGGTTTGATTTTAGATTATTATAATTTTAATCAATATTTAAAAGAATTTGATGCACAAATTGGTATTAATTATATTAAAGTATTACATATTAACGATTCTAAAAACCCTTGTGGTGCAAACAAAGACCGCCACGAAAATTTAGGATATGGAACGATAGGTTTTGCAAATTTAATTAATATTATTTACCATCCCTTATTAAATAACATCCCTAAAATTTTAGAAACGCCTTGGTTTAATGTTAATGATGAATTAATTCCTTTATATAAGCATGAAATAAAAATGATTCGCGACTGCAAGTGGTATGACATTAAAAACAAATTATTATCGAAGAAATAA", "aa": "MNNMDKYNLIIGSHVSLKAKDFFYGSVKEALSYGSNTFMVYTGAPQNTKRQPIKSFKIEEAHNLLKKHNINLDDLIVHAPYIINPCSSKKNVRELAKEFLIQEIQRTESMGITKLVLHPGSRLEQNEDIALEQVYTMLNDIFSTINTNVIVCLETMAGKGSEIGVNIKQLKTIIDNVHSKKNIGVCLDTCHMNDSGLILDYYNFNQYLKEFDAQIGINYIKVLHINDSKNPCGANKDRHENLGYGTIGFANLINIIYHPLLNNIPKILETPWFNVNDELIPLYKHEIKMIRDCKWYDIKNKLLSKK", "aa_hexdigest": "e93d1610e41203a65ca6c7570ebc14ed", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI000165F8B3", "uniref100_id": "UniRef100_Q9PQI5", "db_xrefs": [ "SO:0001217", "UniParc:UPI000165F8B3", "UniRef:UniRef100_Q9PQI5" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQI5", "uniref90_id": "UniRef90_Q9PQI5" }, "psc": { "uniref90_id": "UniRef90_Q9PQI5", "gene": "nfo", "product": "deoxyribonuclease IV", "ec_ids": [ "3.1.21.2" ], "uniref50_id": "UniRef50_Q9PQI5", "go_ids": [ "GO:0003677", "GO:0003906", "GO:0006281", "GO:0006284", "GO:0008081", "GO:0008270", "GO:0008833" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQI5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQI5" ], "product": "Probable endonuclease 4" }, "genes": [ "nfo" ], "id": "NKIECMJPJD_994", "locus": "GCA000828735_00982" }, { "type": "cds", "sequence": "contig_1", "start": 575695, "stop": 576354, "strand": "-", "frame": 3, "gene": "trmN6", "product": "tRNA1(Val) (Adenine(37)-N6)-methyltransferase", "db_xrefs": [ "COG:COG4123", "COG:J", "SO:0001217", "UniRef:UniRef50_A0A448ZXG4", "UniRef:UniRef90_A0AAC9T2A9" ], "nt": "ATGCAAAAAAAATACATTAAAAATACTTTAGGCTTTGATACAAATTTATTTATATATCAAGATAAAAAAATGTTCAATTATTCAATTGATACTATTCTGTTAGCAAATTTTATAAGCTTATCTTCTAAGACGAAAAAAATTTTAGAAATAGGAACAAATAATGCTGCATTGAGTATTTTTTTAGCTTTGCGCAAAGAGGATATAAAAATTGATGCGATTGAAATTCAAATAGAAGCAATTAACCTTGCATTATTAAATGTTAGTCAAAATCATTTTGAAAAACAAATTAATATTATACATGCTGATTTTAATCAATATTGGAAAGATTTTGATAAAAGCGAAAATAATAAATATGATGCAATAATCTGTAACCCCCCTTTTTATAAACAAGATAAAATTATTCCATCAACTAAAAATCCATTAAAAACTTTAGCTTTATATGAAATCACGCTTAATTTTGAACAAATTATGCAAGGGTGTGCTAAGATAATTAAACAAAAAGGAAATTTAGCAATGGTTATTCCTACAACGCGTTTAGTTGATCTTTTAGAAATTATGCGTAAATATCAATTTGAACCAAAACGAATTAAAATGATTTATCCACGAATCTATGAACAATCAAATTTAGTTTTAATAGAAGCGCGTTACAAAAGTGGTTGA", "aa": "MQKKYIKNTLGFDTNLFIYQDKKMFNYSIDTILLANFISLSSKTKKILEIGTNNAALSIFLALRKEDIKIDAIEIQIEAINLALLNVSQNHFEKQINIIHADFNQYWKDFDKSENNKYDAIICNPPFYKQDKIIPSTKNPLKTLALYEITLNFEQIMQGCAKIIKQKGNLAMVIPTTRLVDLLEIMRKYQFEPKRIKMIYPRIYEQSNLVLIEARYKSG", "aa_hexdigest": "1bff32caaceb9e5678dc71c5c71ffb15", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0AAC9T2A9", "query_cov": 1.0, "subject_cov": 0.8588235294117647, "identity": 1.0, "score": 431.0, "evalue": 2.47e-152, "valid": true, "gene": "TrmN6", "product": "tRNA1(Val) (Adenine(37)-N6)-methyltransferase", "uniref50_id": "UniRef50_A0A448ZXG4", "cog_id": "COG4123", "cog_category": "J" }, "pscc": { "uniref50_id": "UniRef50_A0A448ZXG4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZXG4" ], "product": "DNA methylase" }, "genes": [ "trmN6" ], "id": "NKIECMJPJD_995", "locus": "GCA000828735_00983" }, { "type": "tRNA", "sequence": "contig_1", "start": 576417, "stop": 576490, "strand": "-", "gene": "trnG", "product": "tRNA-Gly(tcc)", "amino_acid": "Gly", "anti_codon": "tcc", "score": 79.9, "nt": "GCGGGTGTAGTTCAATGGTAGAACTATAGCCTTCCAAGCTATTAGTGTGGGTTCGATTCCCATCACCCGCTCCA", "db_xrefs": [ "SO:0000260" ], "anti_codon_pos": [ 576456, 576458 ], "id": "NKIECMJPJD_28", "locus": "GCA000828735_00984" }, { "type": "tRNA", "sequence": "contig_1", "start": 576494, "stop": 576579, "strand": "-", "gene": "trnL", "product": "tRNA-Leu(taa)", "amino_acid": "Leu", "anti_codon": "taa", "score": 74.9, "nt": "GCTGGAGTGGCGGAATGGTAGACGCACTGGACTTAAAATCCAGTGACTTCACGGTCGTGAGGGTTCAAGTCCCTCTTCCAGCACCA", "db_xrefs": [ "SO:0000264" ], "anti_codon_pos": [ 576544, 576546 ], "id": "NKIECMJPJD_27", "locus": "GCA000828735_00985" }, { "type": "tRNA", "sequence": "contig_1", "start": 576640, "stop": 576714, "strand": "-", "gene": "trnK", "product": "tRNA-Lys(ttt)", "amino_acid": "Lys", "anti_codon": "ttt", "score": 75.6, "nt": "GCCTCATTAGCTCAGTGGTAGAGCATTTCACTTTTAATGAAGGGGTCAAAGGTTCGAAACCTTTATGGGGCACCA", "db_xrefs": [ "SO:0000265" ], "anti_codon_pos": [ 576687, 576689 ], "id": "NKIECMJPJD_26", "locus": "GCA000828735_00986" }, { "type": "tRNA", "sequence": "contig_1", "start": 576782, "stop": 576856, "strand": "-", "gene": "trnQ", "product": "tRNA-Gln(ttg)", "amino_acid": "Gln", "anti_codon": "ttg", "score": 65.9, "nt": "TGGCCTGTAGCCAAGAGGTAAGGCAGAAGATTTTGATTCTTCCATGCGTAGGTTCGATCCCTACCAGGCCAGCCA", "db_xrefs": [ "SO:0000261" ], "anti_codon_pos": [ 576822, 576824 ], "id": "NKIECMJPJD_25", "locus": "GCA000828735_00987" }, { "type": "tRNA", "sequence": "contig_1", "start": 576862, "stop": 576945, "strand": "-", "gene": "trnY", "product": "tRNA-Tyr(gta)", "amino_acid": "Tyr", "anti_codon": "gta", "score": 67.4, "nt": "GGACAGATAGCGAAGTGGCCAAACGCATCCGGCTGTAACCCGGCTCCGAGAGGTTCGTAGGTTCGAATCCTACTCTGTCCACCA", "db_xrefs": [ "SO:0000272" ], "anti_codon_pos": [ 576909, 576911 ], "id": "NKIECMJPJD_24", "locus": "GCA000828735_00988" }, { "type": "cds", "sequence": "contig_1", "start": 577025, "stop": 577168, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01272.25" ], "nt": "ATGGATTCAGATCCTTTTGCAAACAAAATTTCAATAGACACACCACTTGGAAAAGCTCTTGTAAAACAAAAAAAAGGTACTGAAGTTACAGTTCATACTTTAGCATCACCTTATAAAGTAAAAATTATAAAAATTATTGATTAA", "aa": "MDSDPFANKISIDTPLGKALVKQKKGTEVTVHTLASPYKVKIIKIID", "aa_hexdigest": "6de173cc12348190b0bd6014e9d35bb1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01272.25", "name": "GreA_GreB", "length": 44, "aa_cov": 0.9361702127659575, "hmm_cov": 1.0, "evalue": 8.636237602596197e-15, "score": 50.11517333984375, "start": 2, "stop": 45 } ], "seq_stats": { "molecular_weight": 5153.0432, "isoelectric_point": 9.457012367248538 }, "id": "NKIECMJPJD_996", "locus": "GCA000828735_00989" }, { "type": "rRNA", "sequence": "contig_1", "start": 577607, "stop": 577710, "strand": "-", "gene": "rrf", "product": "5S ribosomal RNA", "coverage": 0.8739495798319328, "score": 82.2, "evalue": 1.1e-13, "db_xrefs": [ "GO:0005840", "GO:0003735", "RFAM:RF00001", "KEGG:K01985", "SO:0000652" ], "nt": "TGTAGTGATTATATCAGAGTGGAAATACCTGTTCCCATCCCGAACACAGAAGTCAAGCACTCTAGAGCCGAAAATAGCGCAAGTAAAATAGGTCATCGCTACGC", "id": "NKIECMJPJD_35", "locus": "GCA000828735_00990" }, { "type": "rRNA", "sequence": "contig_1", "start": 577775, "stop": 580685, "strand": "-", "gene": "rrl", "product": "23S ribosomal RNA", "coverage": 0.9952136752136752, "score": 2800.6, "evalue": 3.9e-06, "db_xrefs": [ "GO:0005840", "GO:0003735", "RFAM:RF02541", "KEGG:K01980", "SO:0001001" ], "nt": "TAATAAGTTACTAAGAGCTTATGGTGAATGCCTTGGGACAAACAGGCGATGAAGGACGTGCTAATCTGCGATAAGCAACGGGTAGCTGATAAGAGGCTTTAATCCGTTGATCTCCGAATGAGGAAACTCAATCTAATGAGAATTAGATTACTGTATAAATGAATTCATAGTTTAACAGAGCGATACCTGGTGAAGTGAAACATCTCAGTAACCAGAGGAAAAGAAAACGAAGTGATTCCCTGTGTAGCGGCGAGCGAAAGGGGAGCAGGCCAAACCGAATTTCGATTCGGGGTTGTAGGACTACAATATGGAATAGAGAATGATAGTAGAATTGGTTGGGAAGCCAAATCATAGAGGGTGATAATCCCGTATACGAAATTATTCTTTTACCTAGTAGGATCCTGAGTAGGGCGGGACACGTGAAATCCTGTCTGAATCCACCCAGACCATTGGGTAAGCCTAAATACTAGTTTGTCACCGATAGAGCATAGTACCGTGAGGGAACGGTGAAAAGAACCCAGAGATGGGAGTGAAATAGAACCTGAAACCATAAGCTTACAAGGTGTTAGAGCACATTAATGTGTGATAGCGTGCCTTTTGAAGTATGAGCCAGCGAGTTATTATAGCATGCGAGGTTAAATCGTAGAGATGGAGCCGTAGGGAAACCGAGTCTTAATAGGGCGTTTTAGTATGTTATAATAGACGCGAAACGGGGTGATCTATCCATGGGCAGGTTGAAGGTGAAGTAACATTCACTGGAGGACCGAACCCACTTTCGTTGAAACGACAGGGGATGACCTGTGGATAGTGGTGAAATTCCAATCGAACTCCGTGATAGCTCGTTCTCGTCGAAATATTTTTAGGAATAGCGTTAGATTATTGGGAATATGGGGGTAAAGCACTGAATCTATGATGGCGCCACCTCGGTGTACTGAATAGAATCAAACTCTGAATACCATATTACCTATTCTAGCAGTCAGACAGTGGGGGATAAGCTTCATTGTCGCGAGGGAAACAGCCCAGATCATTAACTAAGGTCCCTAATATATGCTAAGTGGAAAACGATGTTGAGTTTCATAAACAGCAAGGATGTTGGCTTAGAAGCAGCCACCGTTTAAAGAGTGCGTAACAGCTCACTTGTCGAGAGACTCTGCGCGGAAGATGTAACGGGGCTAAGCATATAACCGAAGTTATGGGTTACATATTATATATGTAGCGGTAGACGAGTGTTGTATATGGGGCGAAGGTAGACTGTGAAGACTACTGGACTTTATACAAGTAAGAATGCTGGCGTGAGTAACGAATGAGAGTGAGAATCTCTCAAACCGATTGACTAAGGGTTCCTGGGCAAGGGTCGTCCTCCCAGGGTAAGTCGGATCCTAAGGCGAGGCTGAAAAGCGTAGTCGATGGAAAACAGGTTAATATTCCTGTACCTACAAATTGTGTGATGGAGTGACGGAGAAGGTTATTATGTGCCGGTTATTGGATTCCGGTTTAAATAACAAGGTTAGTAAGTTGGCAAATCCGCTTACTATTAGGACCAAGTTATGAATACGAGCGACCCCTTCGGGCAGTAGCGAAGACATATACATCATGCTTCCAAGAAAAGCTTCTAGCGTTAAACAATTGGTAGTCCGTACCGAGAACGAACACACGTGGTCAAGGAGAATATCCTAAGGTTAGCGAGTTAACTACAGTTAAGGAACTCTGCAAATTAACCCCGTACGTTAGCAATAAGGGGTGCTCGCTGTAAAAGGTGAGCCGCAGTGAATAGCGAGGGGGGACTGTTTAACAAAAACACAGCTCTATGCTAAGTCGTAAGACGATGTATATGGGGTGACACCTGCCCAATGCTGTAAGGTTAAAGAAGAATGTTAGCGCAAGCGAAGCTTTTAACTGAAGCCCCAGTGAATGGCGGCCGTAACTATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAATTCCGTCCCGCTTGAATGGTGTAACCATCTCTTGACTGTCTCAACTGTAGACTCGGTGAAATCCTGGTGAGGGTGAAGACGCCCTCTTGGCGTGATTGGACGGAAAGACCCCATGAAGCTTTACTGTAGCTTAATATTGGGAAATTTTATTACTTGTAGAGCATAGGTAGGAGACTGTGAAGTATACTCGCTAGGGTATATGGAGTCAACGTTGGAATACTACCCTTGTGATAAGATTTCTCTAACCTGCAGCCATGAATCTGGCTGGGGGACAGTGTTAGGTGGGCAGTTTGACTGGGGCGGTCGCCTCCCAAAAGGTAACGGAGGCGCGCAACGGTACCCTCAGCACGGTTGGAAATCGTGTATAGAGTGTAATGGTATAAGGGTGCTTGACTGTGAGACTTACAAGTCGAACAGGTAGGAAACTAGGTCATAGTGATCCGGTGGCTCAGAATGGAATGGCCATCGCTCAACGGATAAAAGCTACTCTGGGGATAACAGGCTGATAGTGCCCAAGAGTTCATATCGACGGCACTGTTTGGCACCTCGATGTCGACTCATCTCATCCTGGAGCTGAAGCAGGTTCCAAGGGTTCGGCTGTTCGCCGATTAAAGAGATACGTGAGTTGGGTTCAAACCGTCGTGAGACAGGTTGGTCCCTATCTGTCATGCCCGTAGGAAGATTGAGAAGAGCTGTTCCTAGTACGAGAGGACCGGAATGGACACACCTCTTGTGATCCTGTTGTCGCGCCAGCGGCACTGCAGGGTAGCAACGTGTGGAATAGAGAAACGCTGAAAGCATCTAAGTGTGAAACTAACTTCAAGATTAATCTTCCCTTTCTTAAATGAAGTAAGAATCGTTAAAGACTATAACGTTGATAGGTCGGATGTGTACGCACGGTGACGTGTTTAGCTAACCGATACTAATAATTCGAGGACTTATATAA", "id": "NKIECMJPJD_37", "locus": "GCA000828735_00991" }, { "type": "rRNA", "sequence": "contig_1", "start": 580978, "stop": 582494, "strand": "-", "gene": "rrs", "product": "16S ribosomal RNA", "coverage": 0.9895629484670581, "score": 1449.4, "evalue": 1.5e-44, "db_xrefs": [ "GO:0005840", "GO:0003735", "RFAM:RF00177", "KEGG:K01977", "SO:0001000" ], "nt": "ATTTTAAAGAGTTTGATCCTGGCTCAGGATTAACGCTGGCGGCATGCCTAATACATGCAAATCGAACGAAGCCTTTTAGGCTTAGTGGTGAACGGGTGAGTAACACGTATCCAATCTACCCTTAAGTTGGGGATAACTAGTCGAAAGATTAGCTAATACCGAATAATAACATCAATATCGCATGAGAAGATGTAGAAAGTCGCTCTTTGTGGCGACGCTTTTGGATGAGGGTGCGACGTATCAGATAGTTGGTGAGGTAATGGCTCACCAAGTCAATGACGCGTAGCTGTACTGAGAGGTAGAACAGCCACAATGGGACTGAGACACGGCCCATACTCCTACGGGAGGCAGCAGTAGGGAATTTTTCACAATGGGCGCAAGCCTTATGAAGCAATGCCGCGTGAACGATGAAGGTCTTATAGATTGTAAAGTTCTTTTATATGGGAAGAAACGCTAAAATAGGAAATGATTTTAGTTTGACTGTACCATTTGAATAAGTATCGGCTAACTATGTGCCAGCAGCCGCGGTAATACATAGGATGCAAGCGTTATCCGGATTTACTGGGCGTAAAACGAGCGCAGGCGGGTTTGTAAGTTTGGTATTAAATCTAGATGCTTAACGTCTAGCTGTATCAAAAACTGTAAACCTAGAGTGTAGTAGGGAGTTGGGGAACTCCATGTGGAGCGGTAAAATGCGTAGATATATGGAAGAACACCGGTGGCGAAGGCGCCAACTTGGACTATCACTGACGCTTAGGCTCGAAAGTGTGGGGAGCAAATAGGATTAGATACCCTAGTAGTCCACACCGTAAACGATCATCATTAAATGTCGGCCCGAATGGGTCGGTGTTGTAGCTAACGCATTAAATGATGTGCCTGGGTAGTACATTCGCAAGAATGAAACTCAAACGGAATTGACGGGGACCCGCACAAGTGGTGGAGCATGTTGCTTAATTTGACAATACACGTAGAACCTTACCTAGGTTTGACATCTATTGCGATGCTATAGAAATATAGTTGAGGTTAACAATATGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTTTCGTTAGTTACTTTTCTAGCGATACTGCTACCGCAAGGTAGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATATCTAGGGCTGCAAACGTGCTACAATGGCTAATACAAACTGCTGCAAAATCGTAAGATGAAGCGAAACAGAAAAAGTTAGTCTCAGTTCGGATAGAGGGCTGCAATTCGTCCTCTTGAAGTTGGAATCACTAGTAATCGCGAATCAGACATGTCGCGGTGAATACGTTCTCGGGTCTTGTACACACCGCCCGTCAAACTATGGGAGCTGGTAATATCTAAAACCGCAAAGCTAACCTTTTGGAGGCATGCGTCTAGGGTAGGATCGGTGACTGGAGTTAAGTCGTAACAAGGTATCCCTACGAGAACGTGGGGATGGATCACCTCCTTT", "id": "NKIECMJPJD_32", "locus": "GCA000828735_00992" }, { "type": "cds", "sequence": "contig_1", "start": 582758, "stop": 582892, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAAAAGATCAGGTTGTAGTTAATTCTCTAAATATTGAACAAAAAGTTAAATATCGTGAACAAGCAACACGAATTGTTTTTGAGAAACAAGCTGAACATAATGATCATCTAGTAACTTCTGGAAATTTCTAA", "aa": "MQKDQVVVNSLNIEQKVKYREQATRIVFEKQAEHNDHLVTSGNF", "aa_hexdigest": "c7ad849fee22b7cc233f28e78d61effd", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5172.7444000000005, "isoelectric_point": 6.70941104888916 }, "id": "NKIECMJPJD_997", "locus": "GCA000828735_00993" }, { "type": "cds", "sequence": "contig_1", "start": 583316, "stop": 583483, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGTTCCAATTGTTTTTGTTATTTTATCATTTATTTTATTATTTTTATATTATTTTTTAAATAAAAAATCACGATCTAAAGCTGATTTTCCTATTAGTGAAAAATTTTATTATCACACTTCAATTACCACTCTTTATTTAGTAGGATCCTTAATTATTATTAGTTGA", "aa": "MVPIVFVILSFILLFLYYFLNKKSRSKADFPISEKFYYHTSITTLYLVGSLIIIS", "aa_hexdigest": "297ea67a1026a9f3fd0ab0b681901485", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6454.696299999998, "isoelectric_point": 9.45623874664307 }, "id": "NKIECMJPJD_998", "locus": "GCA000828735_00994" }, { "type": "cds", "sequence": "contig_1", "start": 583731, "stop": 583907, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF04452.19" ], "nt": "TTGACCTATCAAAAAATTGCTTTAGTGATTGGACCAGAAGGTGGTTTTGAACAAATGGAAATTAATTTATTAAAACAATTCGATAATGCTATTTGTATTAGTTTGACAAAAACTATTTTAAGATCAGAAACTGCAAGTCTTTATTTACTTGCAAATCTTGTTAATAAGCTTATTTAG", "aa": "MTYQKIALVIGPEGGFEQMEINLLKQFDNAICISLTKTILRSETASLYLLANLVNKLI", "aa_hexdigest": "25b5a90a02488407201a55a01cbe9199", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF04452.19", "name": "Methyltrans_RNA", "length": 51, "aa_cov": 0.8793103448275862, "hmm_cov": 0.9803921568627451, "evalue": 1.918019905212084e-17, "score": 58.883365631103516, "start": 3, "stop": 53 } ], "seq_stats": { "molecular_weight": 6485.629899999998, "isoelectric_point": 6.068153572082519 }, "id": "NKIECMJPJD_999", "locus": "GCA000828735_00995" }, { "type": "cds", "sequence": "contig_1", "start": 584175, "stop": 584435, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF20260.4" ], "nt": "ATGCAACGTTACTTTGTTTCTAAAATTAAAGACAAAAAAGTTTATTTTTATGATTCTGATGTTCATCATATCAAGAATGTTATGCGTATGAGATTACATGATAAAGTTATTGTTATTAATAAAACAAAAGCATTTGTTGCTGAGATTATTAATCTTGAACCATTACATGCAACAATTATTAAACCATATACTCTTAATTGTGAATTAGCTACTAAAATTGATTTATTTCAAGCAAGCATTAAACCAAATAATTTTGAGTGA", "aa": "MQRYFVSKIKDKKVYFYDSDVHHIKNVMRMRLHDKVIVINKTKAFVAEIINLEPLHATIIKPYTLNCELATKIDLFQASIKPNNFE", "aa_hexdigest": "2d0714b4f0304a1e911772b5006c56f6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF20260.4", "name": "PUA_4", "length": 44, "aa_cov": 0.5, "hmm_cov": 1.0, "evalue": 2.2496722602148737e-12, "score": 42.473167419433594, "start": 17, "stop": 59 } ], "seq_stats": { "molecular_weight": 10179.905799999988, "isoelectric_point": 9.443796348571777 }, "id": "NKIECMJPJD_1000", "locus": "GCA000828735_00996" }, { "type": "cds", "sequence": "contig_1", "start": 584441, "stop": 584587, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01029.23" ], "nt": "TTGTTGCTAAGCGCATATGGTGAATATCTGGTTTTAAAAACTCCTAAGAAAATTATTATTGATCAAACTTTAATTACAACACATAATTATTCTAACAACGAATCATATAAGTTTATTAATGCAATTTTAGATCAATTATTAAATTAA", "aa": "MLLSAYGEYLVLKTPKKIIIDQTLITTHNYSNNESYKFINAILDQLLN", "aa_hexdigest": "ec8f4e5b7d4756b4cf5b068a79f61546", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01029.23", "name": "NusB", "length": 46, "aa_cov": 0.9375, "hmm_cov": 1.0, "evalue": 1.655672466915484e-08, "score": 30.543079376220703, "start": 2, "stop": 46 } ], "seq_stats": { "molecular_weight": 5559.3877999999995, "isoelectric_point": 6.516783714294434 }, "id": "NKIECMJPJD_1001", "locus": "GCA000828735_00997" }, { "type": "cds", "sequence": "contig_1", "start": 584827, "stop": 585129, "strand": "-", "frame": 3, "gene": null, "product": "Asp23/Gls24 family envelope stress response protein", "db_xrefs": [ "RefSeq:WP_006688898.1", "SO:0001217", "UniParc:UPI00000C1C0A", "UniRef:UniRef100_A0AAC9T0B7", "UniRef:UniRef50_A0AAC9T0B7", "UniRef:UniRef90_A0AAC9T0B7" ], "nt": "ATGCAGTACATTACCATTAATAATACAAAATCAGGGAAGATTTTAATCTCAAAATCAAATATTAAAAAGTTTATTGAACAAAAATTTAATTTGATTGTTAATAAAAAATTTGTAATTAAGAATATCGATATTGTTCAGTATGATGAATCCCTTGTTGATATATCTATTATTATTTCATTATTTGATATTCATCAAAAAGTTGATCTTGATGAAGTAAGAGATGTACAAAATCATTTAGCTTCTTTTATTTATTCAAATTTAGGCGTTGATACTAAAAGTGTAAATATAGGGATTGATTTATAA", "aa": "MQYITINNTKSGKILISKSNIKKFIEQKFNLIVNKKFVIKNIDIVQYDESLVDISIIISLFDIHQKVDLDEVRDVQNHLASFIYSNLGVDTKSVNIGIDL", "aa_hexdigest": "0c4a2bc01eee682c28658e6d5cdca108", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI00000C1C0A", "ncbi_nrp_id": "WP_006688898.1", "uniref100_id": "UniRef100_A0AAC9T0B7", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1C0A", "RefSeq:WP_006688898.1", "UniRef:UniRef100_A0AAC9T0B7" ] }, "ips": { "uniref100_id": "UniRef100_A0AAC9T0B7", "uniref90_id": "UniRef90_A0AAC9T0B7" }, "psc": { "uniref90_id": "UniRef90_A0AAC9T0B7", "product": "Asp23/Gls24 family envelope stress response protein", "uniref50_id": "UniRef50_A0AAC9T0B7" }, "pscc": { "uniref50_id": "UniRef50_A0AAC9T0B7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T0B7" ], "product": "Asp23/Gls24 family envelope stress response protein" }, "genes": [], "id": "NKIECMJPJD_1002", "locus": "GCA000828735_00998" }, { "type": "cds", "sequence": "contig_1", "start": 585234, "stop": 585353, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCGTTATGATGGTGAATTAATGGGTGTAAGTTTACCAGATCAATTAGTCGTAACAATTATTGAAGCAGAAGAAGCGGTTCAGGGTAATTCAGTTCAAAATGCTACAAAACGTGCATGA", "aa": "MRYDGELMGVSLPDQLVVTIIEAEEAVQGNSVQNATKRA", "aa_hexdigest": "19c4709bcce88799401993e2199a5d16", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4233.7339, "isoelectric_point": 4.3588346481323255 }, "id": "NKIECMJPJD_1003", "locus": "GCA000828735_00999" }, { "type": "cds", "sequence": "contig_1", "start": 585393, "stop": 585698, "strand": "-", "frame": 1, "gene": "efp", "product": "elongation factor P", "db_xrefs": [ "COG:COG0231", "COG:J", "GO:0003746", "GO:0005737", "GO:0006414", "GO:0043043", "KEGG:K02356", "SO:0001217", "UniRef:UniRef50_P75085", "UniRef:UniRef90_Q9PQJ3" ], "nt": "ATGGCAACAATTATTCAAGCAAAAGATTTACGTGCTGGCCACACTTTTTTATATAAAGGTAATATTTACCAAGTAATAGAAAATTCGTTTAATAAAACAGCTATGCGTGAAGGAATTGTAAAATGTAAAGTTAAAAATTTACGTACTGGTGCAATCACTATTGAAGTTTTAACTGGAGAAAAACTTGAACAAGCAGTGATTGAAAAATCAAAAATGACTTTTAGTTATGATGATGGTAGTGGTTATGTGTTTATGGATAATGATACATATGAACAAATTTCAATTCCTTACAGTCAATTAAGTTGA", "aa": "MATIIQAKDLRAGHTFLYKGNIYQVIENSFNKTAMREGIVKCKVKNLRTGAITIEVLTGEKLEQAVIEKSKMTFSYDDGSGYVFMDNDTYEQISIPYSQLS", "aa_hexdigest": "f573559ed9b68600fb37e17508b9c667", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "genes": [ "efp" ], "truncated": "5-prime", "pseudogene": { "start": 585132, "stop": 585698, "observations": { "mutation": [ 585221, 585236, 585395 ], "stop": [ 585221, 585236, 585395 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQJ3", "query_cov": 1.0, "subject_cov": 0.5372340425531915, "identity": 1.0, "score": 201.0, "evalue": 3.49e-64, "gene_start": 1, "gene_end": 101, "reference_start": 1, "reference_end": 101, "reference_sequence": "MATIIQAKDLRAGHTFLYKGNIYQVIENSFNKTAMREGIVKCKVKNLRTGAITIEVLTGEKLEQAVIEKSKMTFSYDDGSGYVFMDNDTYEQISIPYSQLSWEKNFIEEGTEVSVMRYDGELMGVSLPDQLVVTIIEAEEAVQGNSVQNATKRAWLESKWEFQVPQFIKSGEKVIINPSNGQYVGRAK" }, "paralog": false, "identity": 0.9840425531914894, "score": 357.0, "evalue": 2.56e-128, "description": "Internal stop codon at 585221, 585236, 585395. Nonsense mutation around 585221, 585236, 585395.", "psc": { "uniref90_id": "UniRef90_Q9PQJ3", "gene": "efp", "product": "elongation factor P", "uniref50_id": "UniRef50_P75085", "cog_id": "COG0231", "cog_category": "J", "kegg_orthology_id": "K02356", "go_ids": [ "GO:0003746", "GO:0005737", "GO:0006414", "GO:0043043" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQJ3", "UniRef:UniRef50_P75085", "COG:COG0231", "COG:J", "KEGG:K02356", "GO:0003746", "GO:0005737", "GO:0006414", "GO:0043043" ] }, "pscc": { "uniref50_id": "UniRef50_P75085", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75085" ], "product": "Elongation factor P" } }, "id": "NKIECMJPJD_1004", "locus": "GCA000828735_01000" }, { "type": "cds", "sequence": "contig_1", "start": 585841, "stop": 586254, "strand": "+", "frame": 1, "gene": "recU", "product": "Holliday junction resolvase RecU", "db_xrefs": [ "COG:COG3331", "COG:R", "EC:3.1.21.10", "GO:0000287", "GO:0003676", "GO:0004519", "GO:0005737", "GO:0006281", "GO:0006310", "GO:0007059", "KEGG:K03700", "SO:0001217", "UniRef:UniRef50_Q9PQJ4", "UniRef:UniRef90_Q9PQJ4" ], "nt": "ATGAATAATAAAAACAATGGAATGCATCTTGAAGTATTAATTAATAAATCAATTAGCTTGTTTAATTTACAATATCAAGCTTTTTTTAAAAAAAGGTGTCTTGATATTATTATTAAAAATATTGATAATTCTTATGTTCAAGGAAAAATCAAGCAAAAAAGTGAAACTGATTATTACGGATTCTATAAAGGTGATTATTTTGATTTTGAAGCTAAACAAACAAATAAAAACTCATTTTTAATAAAACAAATACAACCGCACCAACTAGCACATTTGTATTTAATTCATAAAAATTCAGGTTTCTCTTTTTTAATTATTTGTTTCATTAATTACAATCTATATTTTATTATCACTTTTCAACAATTAATTAACTATTATCAAAAAACTAAGCGGAAATCCATTCCCTTTCAATGA", "aa": "MNNKNNGMHLEVLINKSISLFNLQYQAFFKKRCLDIIIKNIDNSYVQGKIKQKSETDYYGFYKGDYFDFEAKQTNKNSFLIKQIQPHQLAHLYLIHKNSGFSFLIICFINYNLYFIITFQQLINYYQKTKRKSIPFQ", "aa_hexdigest": "3a2319984840fddcfbc4e5be37f0def2", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_Q9PQJ4", "query_cov": 1.0, "subject_cov": 0.8058823529411765, "identity": 0.993, "score": 276.0, "evalue": 1.65e-93, "valid": true, "gene": "recU", "product": "Holliday junction resolvase RecU", "ec_ids": [ "3.1.21.10" ], "uniref50_id": "UniRef50_Q9PQJ4", "cog_id": "COG3331", "cog_category": "R", "kegg_orthology_id": "K03700", "go_ids": [ "GO:0005737", "GO:0004519", "GO:0000287", "GO:0003676", "GO:0007059", "GO:0006310", "GO:0006281" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQJ4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQJ4" ], "product": "Holliday junction resolvase RecU" }, "genes": [ "recU" ], "id": "NKIECMJPJD_1005", "locus": "GCA000828735_01001" }, { "type": "cds", "sequence": "contig_1", "start": 586350, "stop": 586988, "strand": "-", "frame": 1, "gene": null, "product": "Sensor histidine kinase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAP9AA00", "UniRef:UniRef90_A0A2C9DYP1" ], "nt": "GTGCTTGGATATTCTAGTTTATCAGTTACTATAATAACACCTTTAATTTATAAGATATCATATAATAAACAATACTCATTTATTACTAAAAGTATACGAGAATTAAAAACAATTGATACTAATTTTGTTTCATCTTATTCAGATTTTTATATTCCTCAGAAATCAAGTATATCTAATGACTTATATAATTATAATTTGATTAACAATTATCATGATTTTGAAAAATTAATAAAAAACGATTTGAATTTTGATAAATATAATAGTGCTTTGATTTATGAAAAAATAAAGGTTAGTTTATTAGAGAAATATAATGAAAATTTTTTCAAAAATAACGATCTGTTATATGTTTCTTATAATTCATTAGACTCAAAATATTTTTTAACTTCAATTTTGAGTTTTGATTACAATAATTTATATTTACCATTTTTAAACACTAAATCGATTTTTAATAATGAGTATGATAATAAAAATTTTTATACAGATTCAGCTCTAAGTACTACTAAAACAGAAGTTAAATTTTTTAGTATTAATAAAAAATATAACATAAAACTTAAACAAATTAAAATTTATAGTATTGAAACGGCAAAGGATTTATTTCTATACAAAAACTATGTTCGAAAGAATAATAAACCTATTTAA", "aa": "MLGYSSLSVTIITPLIYKISYNKQYSFITKSIRELKTIDTNFVSSYSDFYIPQKSSISNDLYNYNLINNYHDFEKLIKNDLNFDKYNSALIYEKIKVSLLEKYNENFFKNNDLLYVSYNSLDSKYFLTSILSFDYNNLYLPFLNTKSIFNNEYDNKNFYTDSALSTTKTEVKFFSINKKYNIKLKQIKIYSIETAKDLFLYKNYVRKNNKPI", "aa_hexdigest": "23a413df1695353f1b4cf61c1b48b28b", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A2C9DYP1", "query_cov": 1.0, "subject_cov": 0.8617886178861789, "identity": 0.981, "score": 299.0, "evalue": 2.32e-100, "valid": true, "uniref50_id": "UniRef50_A0AAP9AA00" }, "pscc": { "uniref50_id": "UniRef50_A0AAP9AA00", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAP9AA00" ], "product": "Sensor histidine kinase" }, "genes": [], "id": "NKIECMJPJD_1006", "locus": "GCA000828735_01002" }, { "type": "cds", "sequence": "contig_1", "start": 587327, "stop": 587701, "strand": "-", "frame": 2, "gene": "tACO1", "product": "putative transcriptional regulatory protein UPA3_0303", "db_xrefs": [ "COG:COG0217", "COG:KJ", "GO:0003677", "GO:0005829", "GO:0006355", "SO:0001217", "UniRef:UniRef50_Q7NBX7", "UniRef:UniRef90_Q9PQJ6" ], "nt": "ATGAAATGTGCAAAAGAAATTAAAGCTGCCGCAAAAATGGGTGGTCCTAACCCAGAGGCAAATTCGCGTTTAAAAGTTGCTATTGAACGCGCTTTAAATAATAATTTGAGTCGTGATTCAATTGAACGAAACATTAATGGTGCCAGTAAAGATATTGATAATTTAAAAGAATTAACATACGAAGGATATGGACCAAATGGATTAGCAATTATTGTTCGTGCTTTAACTGATAATGAGCAGCGGACAATATCAGCTATTCGTGGTTATTTTTCGAAATTACAAGGTCAGATTGCTAAACCTAATTCTGTTAGTATGTTGTTTAATGAATGTGGACAGTTATTAATTAATAAAGAAACTAAATCTTTAGATGAATGA", "aa": "MKCAKEIKAAAKMGGPNPEANSRLKVAIERALNNNLSRDSIERNINGASKDIDNLKELTYEGYGPNGLAIIVRALTDNEQRTISAIRGYFSKLQGQIAKPNSVSMLFNECGQLLINKETKSLDE", "aa_hexdigest": "283fb7d0e1f1f3725da69da0d28f9788", "start_type": "ATG", "rbs_motif": null, "genes": [ "tACO1" ], "truncated": "both", "pseudogene": { "start": 587057, "stop": 587767, "observations": { "mutation": [ 587089, 587329, 587704 ], "stop": [ 587089, 587329, 587704 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQJ6", "query_cov": 1.0, "subject_cov": 0.5254237288135594, "identity": 0.9440000000000001, "score": 227.0, "evalue": 1.35e-73, "gene_start": 1, "gene_end": 124, "reference_start": 23, "reference_end": 146, "reference_sequence": "MPRKHLIASGINKKQQQQAKIWMKCAKEIKAAAKMGGPNPEANPRLKVAIERALNNNLSRDSIERNINGASKDADNLKELTYEGYGPNGLAIIVRALTDNEQRTISAVRGYFSKLQGQIAKPNSVSMLFNEYGQLLIDKKTKTLDEWFELLVDQDIVDINEDDEIIEILVQPKDFSATKLILENNNANIQSAEIKLIPTDFISLDDHARERLVRFVNACENDDDISWVITNYEEEL" }, "paralog": false, "identity": 0.8940677966101694, "score": 414.0, "evalue": 1.45e-149, "description": "Internal stop codon at 587089, 587329, 587704. Nonsense mutation around 587089, 587329, 587704.", "psc": { "uniref90_id": "UniRef90_Q9PQJ6", "gene": "TACO1", "product": "Probable transcriptional regulatory protein UPA3_0303", "uniref50_id": "UniRef50_Q7NBX7", "cog_id": "COG0217", "cog_category": "KJ", "go_ids": [ "GO:0005829", "GO:0003677", "GO:0006355" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQJ6", "UniRef:UniRef50_Q7NBX7", "COG:COG0217", "COG:KJ", "GO:0005829", "GO:0003677", "GO:0006355" ] }, "pscc": { "uniref50_id": "UniRef50_Q7NBX7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q7NBX7" ], "product": "Probable transcriptional regulatory protein MYCGA1330" } }, "id": "NKIECMJPJD_1007", "locus": "GCA000828735_01003" }, { "type": "cds", "sequence": "contig_1", "start": 587777, "stop": 587872, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTAATATATTTTTATATCAATATTATAATGAAGGTATAAAAATCGATATTGATTTTTTAAATCATTTTTTATTAAAAGATGCACTAAAATAG", "aa": "MINIFLYQYYNEGIKIDIDFLNHFLLKDALK", "aa_hexdigest": "ab41306f552fc2a2f6b592d2385aee72", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3806.4269000000004, "isoelectric_point": 5.410640144348144 }, "id": "NKIECMJPJD_1008", "locus": "GCA000828735_01004" }, { "type": "cds", "sequence": "contig_1", "start": 587891, "stop": 588034, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACCAAAAATTTTATTGATTATAAAAAAAATGAAAATAAAATTGCTACTCGTTCGAAAATTTTATATGATATGATTGTTAAAAATGAATCATTAAATATTAATCATAGTGAATATGAATCTTTATTAGTCAATAATGAATGA", "aa": "MTKNFIDYKKNENKIATRSKILYDMIVKNESLNINHSEYESLLVNNE", "aa_hexdigest": "65a55b04e9c122c946b671e2d50c96ae", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5606.300399999998, "isoelectric_point": 6.558503532409667 }, "id": "NKIECMJPJD_1009", "locus": "GCA000828735_01005" }, { "type": "cds", "sequence": "contig_1", "start": 588086, "stop": 588616, "strand": "-", "frame": 2, "gene": null, "product": "Lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9X723", "UniRef:UniRef90_A0AAC9X723" ], "nt": "ATGCAAATTAATATTAAAAAAATAATAATAAAAATAAGCACTTTTTTAGGGTTAGGAATAATTCCTATTTTAGCAAGTTCTTGTTCTATTATTATTCCTGATTCGATTCCTTTAAAATCAAATGTTTCTTCAATTACTAATGATATTTCAAAATTAGCTAATGATTATATTGATAATCAACACAAAATTATTGAGTCTATTAATAAAAATAAAAAAATTAGTTTAACGAATGAAATGATTCAAAAAGCAAAATTTTATGCTGGATATATTTCTCGTCTGCGTTTTATTCAAAAATTAAGATATGAAATTCAAAACGAAAAAAAAACATTGAATAAAATAATTAAAGTTGTGAATGATGCTGATATTAGTACCTTATATGATTTTAAAGATAAAAATCTAACTATTTATACGAAAACATTTTATTTCCATAATTATCAATTTGTCAATGCTAATAAAAAAAATTATTTTATTTCTAAAAAAACTATTAAATCTGTTATTACAGAAAATTTATTAGATATAGGATTTACTTGA", "aa": "MQINIKKIIIKISTFLGLGIIPILASSCSIIIPDSIPLKSNVSSITNDISKLANDYIDNQHKIIESINKNKKISLTNEMIQKAKFYAGYISRLRFIQKLRYEIQNEKKTLNKIIKVVNDADISTLYDFKDKNLTIYTKTFYFHNYQFVNANKKNYFISKKTIKSVITENLLDIGFT", "aa_hexdigest": "ff6ccb1aed7cf7735d8137c874e7eff5", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 587783, "stop": 588616, "observations": { "mutation": [ 587893, 588088 ], "stop": [ 587893, 588088 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAC9X723", "query_cov": 1.0, "subject_cov": 0.6308243727598566, "identity": 1.0, "score": 329.0, "evalue": 2.31e-112, "gene_start": 1, "gene_end": 176, "reference_start": 1, "reference_end": 176, "reference_sequence": "MQINIKKIIIKISTFLGLGIIPILASSCSIIIPDSIPLKSNVSSITNDISKLANDYIDNQHKIIESINKNKKISLTNEMIQKAKFYAGYISRLRFIQKLRYEIQNEKKTLNKIIKVVNDADISTLYDFKDKNLTIYTKTFYFHNYQFVNANKKNYFISKKTIKSVITENLLDIGFTWTIIYNDQTKTYIRPTDFMTKNFIDYKKNENKIATRSKILYDMIVKNESLNINHSEYESLLVNNEWKRYYENMINIFLYQYYNEGIKIDIDFLNHFLLKDALK" }, "paralog": false, "identity": 0.9927797833935018, "score": 541.0, "evalue": 2.07e-198, "description": "Internal stop codon at 587893, 588088. Nonsense mutation around 587893, 588088.", "psc": { "uniref90_id": "UniRef90_A0AAC9X723", "product": "Lipoprotein", "uniref50_id": "UniRef50_A0AAC9X723", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAC9X723", "UniRef:UniRef50_A0AAC9X723" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAC9X723", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9X723" ], "product": "Lipoprotein" } }, "id": "NKIECMJPJD_1010", "locus": "GCA000828735_01006" }, { "type": "cds", "sequence": "contig_1", "start": 589219, "stop": 591513, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTAGCGCGTGTAATATTTAAACAAATTTCTCCACAAAAAAAATATGATCAAATTCAGCAAATAAATAAAGACAAAGTGGTCACTTTTATTAAGAAGCTTGGTGACAATCTGACAAAATTTGATTTATATAATAAATTACTCGATAATTTATTTAGTTCAATTACAAATCAAACAAATTTAACAAAATTATTGAGTGAACAGCAAGATAGTGTAATTAATTTTATTAAAACGATTTTTGATTTTAGTGATCCTAAACAAATTATTTCACTAATTGATGTATTAAAAGTCAACGAGATTACAAGTAATGATTATATTGAAGTTATTAAATCAATTTTAGATGAAATTGATTTTAATCATTTCTTTAATCAAGCTGTCAAAAACAGTAATCTTTCAAATTATCCATTGAATAAACATGAAAATGATAATGATAGTAAATTAGATATAAATAAGTACTATATTTATGTAAAAACATTATTTGCGCATGAAATGGATTCTACATCAAAAGAAAAATTAAAAACAATTATTGATGAACTAACAAAAAAAATTGTTGAAAATAATAATAAGGGCTTGTTGTACAATTTGGGCAATGGATTAGGTGAAAAACTAAGTAATTTAATTATTAATACATTCCCTGGGTTAACTAATTTAAAACAAAACTATAAAACACTAGTACAAAAAGTATTTACTAATCAAAACTTGTTTGAGCAAGTTCAAAATTTGATCACTTATGCAATCAATAGTTTGATTGATCATAAAAATGAATATCAAAATTGTAAAACTTTTGGTGAATTATTAACAACTTACATTAATAAGAATCAAACACAAATTATTGATAAATTAAAAGAAGCAATTAATAAAATAATCAGTGGTTCATCTTCATTAATTGATGAAATTTCAACATCATTTGCTAATACAATTAAAACATATTTTAAATTAGAAAATTTAACAGTAGAAGATATCAATAAACTAAAGTCATTTATTAATTTTGTTATCAAAAATATTTTCAATCTTGAATATGTTAATGATGGTTTAGTTAAATTAATTACGACTTTAAGTTCAAACGCCATAAAAATTGTTGATCAAAACACTAATTTTGTAACTGTTATGCAAAATGATGTTATTTCATATTTAACATCTCCGCAACAAATTGCAAAATTACTTCAAATTGTTGATTTGGATAAAAATGATGACATTTTAAATTTCTTAAAAGTTATTTTATCTCATTTGCCTCAAAACGCATATACTATTTTATTTGGTAATAAAATTAATCTTATAACAAAGAACACAAATCAAAAAACTAATAGAAGTGTAATTCAAAAATATACAATATTAGATTATGTAAAAACTATTTTAAATTCTTCTTACTTAAAAAAACAAGATACAATTGTAAAAATTAAACATATTTTTAAAGATCTTTTAGTAACTATATCTAAATCATCACAATTAAATAATTATATTGGTGAGTTGATTAGTAATACTTTCGCACAAAAACTTTCAAATCTTAGTGGTGTGGATAAACAATATAGTGAATCATTTTTAAAAGAATCATATAAATTTATTATAAGTGATGAAAAATTAATCAAGATTATTGATGAAGTAGTTAATGATTTAGTTGATCATAAAGATGAATATTTAAAAGAAATTACAAACAATAATCAACCATCAATTAATATTATTTTAAATATGTTAATTAAACGAAATGTTGATCAACAAAAAGTTGATTTTGTACCTTTTATAAAACGTTATTTAGTTATTGATTCTTCAACACAATTTATGGCTCAATACCTTTTTAAAACTTTAAAATTAGAAAATACTAATGATCAAGATGTAAAAATTGTTCAAACATTTGTAAAAGAATTAATTAATCAAATTGATCAATTAGATTTTGTAAATTTATTCATTGATAAAATATTTACTTTAATAAAAGAAAATGGACTTGATTTATTTACAAAAGAAGAGGGTATAAAAAAAGTAAATGAAGCTATGAAAACGTTTGGTTTAACTGAAACAACTAATTTATTAAAAATTGCAAAACTTGTCGAACCAAATAAAATTAAACCCCAAACCATAGGTGATCTAATTAATTTAGTATTTGAAAAATCACCATTAGCTGAAGATTTCGAAAGCATAACACAAGATAAAAATCCTTTATATTATGGTTTAAAAAATCTTAAAGAAGATAGTTTAAATGCTGTTTTATTTGCTAGTGGCAAAAAGAAAACAGGAGAAGCAGCAAAAGTTAGTGAAATTAATATGTTAGATGCTATCCAACATTTAATTAATAAATTATGA", "aa": "MLARVIFKQISPQKKYDQIQQINKDKVVTFIKKLGDNLTKFDLYNKLLDNLFSSITNQTNLTKLLSEQQDSVINFIKTIFDFSDPKQIISLIDVLKVNEITSNDYIEVIKSILDEIDFNHFFNQAVKNSNLSNYPLNKHENDNDSKLDINKYYIYVKTLFAHEMDSTSKEKLKTIIDELTKKIVENNNKGLLYNLGNGLGEKLSNLIINTFPGLTNLKQNYKTLVQKVFTNQNLFEQVQNLITYAINSLIDHKNEYQNCKTFGELLTTYINKNQTQIIDKLKEAINKIISGSSSLIDEISTSFANTIKTYFKLENLTVEDINKLKSFINFVIKNIFNLEYVNDGLVKLITTLSSNAIKIVDQNTNFVTVMQNDVISYLTSPQQIAKLLQIVDLDKNDDILNFLKVILSHLPQNAYTILFGNKINLITKNTNQKTNRSVIQKYTILDYVKTILNSSYLKKQDTIVKIKHIFKDLLVTISKSSQLNNYIGELISNTFAQKLSNLSGVDKQYSESFLKESYKFIISDEKLIKIIDEVVNDLVDHKDEYLKEITNNNQPSINIILNMLIKRNVDQQKVDFVPFIKRYLVIDSSTQFMAQYLFKTLKLENTNDQDVKIVQTFVKELINQIDQLDFVNLFIDKIFTLIKENGLDLFTKEEGIKKVNEAMKTFGLTETTNLLKIAKLVEPNKIKPQTIGDLINLVFEKSPLAEDFESITQDKNPLYYGLKNLKEDSLNAVLFASGKKKTGEAAKVSEINMLDAIQHLINKL", "aa_hexdigest": "f8fec47476c2066f475b823793461478", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 87859.16530000043, "isoelectric_point": 8.400955772399904 }, "id": "NKIECMJPJD_1011", "locus": "GCA000828735_01007" }, { "type": "cds", "sequence": "contig_1", "start": 592456, "stop": 592701, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACATCGTTTATTAGTCATAAATTAAGTGATTTATTAATTAAAAAAATTCAAGTATTAACTCCACAGAAAAATGAATATCAACAATTAGTTAACAATTTATTAAATGGTATTGATTTTAAAAATGTTGTCCAAAATTTAATTGTTGAAGGCTTTGCAAAAATAATTGATCATCAACAACAATTAAAAAACTCACAAAATTTTGGAGATTTAATTCAAAAAGTTATCAATATAGATGATGTATGA", "aa": "MTSFISHKLSDLLIKKIQVLTPQKNEYQQLVNNLLNGIDFKNVVQNLIVEGFAKIIDHQQQLKNSQNFGDLIQKVINIDDV", "aa_hexdigest": "b26e49cd478d6b7a202daf8d599d061b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9304.637499999995, "isoelectric_point": 6.705887031555175 }, "id": "NKIECMJPJD_1012", "locus": "GCA000828735_01008" }, { "type": "cds", "sequence": "contig_1", "start": 592708, "stop": 593787, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCAATAAATAATTTAATAAACTCTAAAATTTTGCCTACTAATTCTTTATTATCTGTTAGAAATATTTCAATTTCAAAAACAAAACCAGAATTAAATAAAATTGAAAACATCATTTTAGATTTATTTAAAGCATTATCACAAAAAGCTAAAACATTTGATAATAATACCTTAACTAAAGCAAAAAACATTATCGAATTTACTTTTAATAAGCTTTTAACATCAAATAATATTAAAAATTTTATTAATAATCTATTATCAAATTTAAGTCCCATTAGATCTTTTTTGAAATCAATTAATGTTCAAGATAAAAAGCAAACACAACTTATTGAACATGTAATTAATTCTCTTTTAAAAGATAAACAATTACAAACAATTTTTAACGCCTTAATTGAACCTATTTTATCAAATAACAATAATTTTGATAATGTTAAAACTTTATCAGAATTTATTAGTCAGATTATTATTAAAAGTGGAGAGAAAATTAAGCCAGCTATTACTAAAATTATTAAAAATTTATTATTAGATGATGAAATACAAACATTATTATTACGTTATATTATTCCACAAATTAATACAACAAAAACATACGATAGTATTAATAATTCAGCTATTAATAAAATCAAAATTTTAATTACTAAGTTAGCAAATAATTTAGATAAGTTTAGTATGTATAATGATCTTTTAGATAGTTTATTTACAAAATTAAGCGATAAATCTATTTTAAATAAGTTATTAAATGATGATTTTTTATCAATTGGAAGTATTTTAAAAGAAATTTTTAATATAAATCAACCATCACAACTAATAAAAGTTATTGATATTTTTAGTATCAAAGACATTACTGCCCAAGATTATGTAAATGTTTTGAATGTTATCTTTAATGATATTGGTTTTAATAATCAACAAACAAAACAAAATATTACCAATGATCAAAAATACGATATTAATATTTACTTTGAATATTTAAAAGCTTTAGTTGAACATAATTTTAGTGAGTTAACTAAAACAAAATTAAAAGAAATCGTCAAATTATTCGTTAAAGATGTAATGAATGATGACCATCATAACCATTTATGA", "aa": "MPINNLINSKILPTNSLLSVRNISISKTKPELNKIENIILDLFKALSQKAKTFDNNTLTKAKNIIEFTFNKLLTSNNIKNFINNLLSNLSPIRSFLKSINVQDKKQTQLIEHVINSLLKDKQLQTIFNALIEPILSNNNNFDNVKTLSEFISQIIIKSGEKIKPAITKIIKNLLLDDEIQTLLLRYIIPQINTTKTYDSINNSAINKIKILITKLANNLDKFSMYNDLLDSLFTKLSDKSILNKLLNDDFLSIGSILKEIFNINQPSQLIKVIDIFSIKDITAQDYVNVLNVIFNDIGFNNQQTKQNITNDQKYDINIYFEYLKALVEHNFSELTKTKLKEIVKLFVKDVMNDDHHNHL", "aa_hexdigest": "0b51348c01d8afdf9e364c7ce8dce672", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 41280.401500000036, "isoelectric_point": 9.232597923278806 }, "id": "NKIECMJPJD_1013", "locus": "GCA000828735_01009" }, { "type": "cds", "sequence": "contig_1", "start": 593818, "stop": 595812, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCAACAATACCTAATTTAACTTTATATAAAGAAATCCGAAAACAAGCTTTTGAATTTTTAACAAACAATATTAATAAAATTTTAGTGGATCCTAAAAACACTTTATTAATATTAAATGATGGGATTAACAAATTATTATCAAACATATTGCCACAATTATCAACCCTGATTGAATTAATTGATAACGATAAAATTAAAGATCAAGATTTTATTGATGTTATTAAAATATTTATAGACAATTTAGATTTTAATAAATTATTTGTCAATATAAAGTCTAATAATAACCAAACAACAGATAGTACTACTTTAAATCAATTTTATTTTAAATTTAAATTAAATTTAGAACCTAAAAAATTAATTTTCGATTTAATAAAAAATCTTTTGAATTCACCAATTTTAAAAGTAAATGATAATAAAAATAATAAAATTAATATTGAATCTAATAAAGCTAAAATTAAGTCTATTCTAACAACTAGTGTTACAAACATTTTTGAAAATTCTAATCTTATGAATTTTTTTAATAATCAATTATCACAATTATTTAGTTCAATTGATTTATTTAAAAATTTAAATTTATCAAATCAACAACGTTTAAACTTTGCTAAGGCTATTGTTGAATTATTAAATAAAGAAAAATTATTATCAAATTTAATTAATGTTTTAATAACTAACTTAATTGATCACGCTGATGAATATGCTGAAGCAACAGATTTAGCAACAATTTTATCATTAACAATAAAATATAATCAAAATGAATTAAAAAATGCGTTTGATAAATTATTAATAGGAACTTTAAAAAATGATAATCTTCAAGATTTAATTTTACGTATTTTTGTTAAATTAATTAATCCTAACAAGTCTTATGATTCAATTAGTGTAGTTTCAATTAATAAATTGAAAACATTTATATCAAAATTTGCTACAGGAATTAGTAAATTGGATTTATATCAACAATTAAAAAATTCAATATTTACAGTTTTTTCTAACAAAACAAAAATGCAACAATTATTATCAAATGATGTGAGTGTAATAAATAAAACACTACTAGAAGCATTTAACATTGACACACCAGAAAAAGTTTTGAAAATTAGTAGTTTATTAGATATTAATGAAATTGATGCTCGTGATTATGCTGATATTATTGAAATTATTTTAGTTGAAATTGGTTTTAATGTTACAAGTATTCAAAATAATGATGTTAATAATAATTTGAAAACAATTGATAAAAAAACAAATGATTTTGCTTTTCAAATTTTGAAAAAAGCTTTAAATAATGGTGTTAGTGATCAACAATTACAAAAAATTAAAAATGTTGTTAATTATTTATTAGATGATATTGTTAAAATTCATGATTCAAGTAGTTTTTTAAGAATTCAATTAGAACAATTATCACATGTTTTAGTTGATAAAATTACCGCAGTTTTACCTAAATCATTAACAATTAAACATAAATATACAAAGATTTTTTCATCGATTTTAAATAATCAAGATTTTTTACAAAAAGCCAAAACTTTATTATCAACAATTTTAAATGAACTGATTGATCATAAAGATAAATATAAAGACATTAATTCGTTTAGTGAATTAATCTCCGTATTCTTTAAAAATAAAGCTAGCGATCTAAAAACACAATTAAAAGATTTACTAAATACAATTTTAAAAAACCAAACACTAATCACTAATATTGGACAAGTAATAATAGAATCATTTAAACTAGAAAACAAAATAAGTATTCTAGATAGTGATCTTGAACAAAATACGATTTCATTTATTAATAAAATTTTTGCTCACATTACAGAATTGCCAATTTATACTAATTTAGTGGATAATTTTTTTAATTTTTTTAGTGAATATACAAAAAGTTCTGACACAAAAACATTGAATTTTAATAAATTTAAAAGTAGTTTATTCCAAGCTATCATACCTAAAATAAGTGATTTTGCATCTCTTTTAGACTTATTTAAATATAATGAAATTAGCGAACAAGAATGA", "aa": "MPTIPNLTLYKEIRKQAFEFLTNNINKILVDPKNTLLILNDGINKLLSNILPQLSTLIELIDNDKIKDQDFIDVIKIFIDNLDFNKLFVNIKSNNNQTTDSTTLNQFYFKFKLNLEPKKLIFDLIKNLLNSPILKVNDNKNNKINIESNKAKIKSILTTSVTNIFENSNLMNFFNNQLSQLFSSIDLFKNLNLSNQQRLNFAKAIVELLNKEKLLSNLINVLITNLIDHADEYAEATDLATILSLTIKYNQNELKNAFDKLLIGTLKNDNLQDLILRIFVKLINPNKSYDSISVVSINKLKTFISKFATGISKLDLYQQLKNSIFTVFSNKTKMQQLLSNDVSVINKTLLEAFNIDTPEKVLKISSLLDINEIDARDYADIIEIILVEIGFNVTSIQNNDVNNNLKTIDKKTNDFAFQILKKALNNGVSDQQLQKIKNVVNYLLDDIVKIHDSSSFLRIQLEQLSHVLVDKITAVLPKSLTIKHKYTKIFSSILNNQDFLQKAKTLLSTILNELIDHKDKYKDINSFSELISVFFKNKASDLKTQLKDLLNTILKNQTLITNIGQVIIESFKLENKISILDSDLEQNTISFINKIFAHITELPIYTNLVDNFFNFFSEYTKSSDTKTLNFNKFKSSLFQAIIPKISDFASLLDLFKYNEISEQE", "aa_hexdigest": "6dc631c7e6f2376d762a497db256f142", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 76206.0590000004, "isoelectric_point": 8.564576530456542 }, "id": "NKIECMJPJD_1014", "locus": "GCA000828735_01010" }, { "type": "cds", "sequence": "contig_1", "start": 596137, "stop": 596277, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTGTTGATGAATTATTAAAAAATCCACAAACTCAAAATTTTGTTACTTCTATAGAAGATTTTATTGCTAATAAATTAGGCGATCTGGTAATTAGTGCAAATAATAGTTTACTAAAATCAAATATGTCTGATATTTGA", "aa": "MSVDELLKNPQTQNFVTSIEDFIANKLGDLVISANNSLLKSNMSDI", "aa_hexdigest": "bd92b876bde28104e852b4c17ed0d215", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5068.686299999999, "isoelectric_point": 4.232879447937012 }, "id": "NKIECMJPJD_1015", "locus": "GCA000828735_01011" }, { "type": "cds", "sequence": "contig_1", "start": 596482, "stop": 597312, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTATTTCTGGAATTCAAAATGATTTAGATAATTTAGATTTAGATAAAAATAATATAATTGGGACACAAAATATTTCTAAAAAAATTTTATATGATGTTTTTAGAAAAAATATTATCAATGATCAGAATTTATATGAATTAATTAAAGGAATGTTTCATTCTCAGCTATTCAATGAAAATAAATCTCAAATTAAATCTTTAATAAAAGCTTTATTAAGTGATTTGGTTAGTTCCCATAAATTATTAGAAATAATTTTTGGTGAATTTGCAACTGATAATTTAAAGAATAATAATGAATTCTTAAAAAATTTACTAATAATCCAAAATAAATTAACTCAAACAAAATCACTAGATAACATTATTAATGTTTTTGTAGATGCTTTGTTTGATAATGATAATGAATATTTATTGCAGGGTCCTTTATCAAATGTTTTAGCAAAAATTGTTAAGACTAATAAAGCATTGTTAACAACTAGAATTGACAATTTATTTAATGATTTATTTAAAGATCAAACAACATTAGATGCAACTTATAACATTCTAATAAGAGTTTTAGGCCAAAATATTCCTGATTTTATAAAAACTCCGCAAGCATCTTTAATAATTAAAAAAATTTTATCAAAAGGTACAAATTTAAAATTAATAAACACTTTAAAATTAAATACGATTGATTTATTAACTGATTCAAAAACATATGCTGGCATATTTGATTCATCAGAAACTTCAATTATCAATCTTATTCAAAAATCCATTGATTTTACTTCAAAATCATTTATTAGCGATGTTTTAGATTTATTAACATCAGATAATGTTGATTTAAACGAATGA", "aa": "MVISGIQNDLDNLDLDKNNIIGTQNISKKILYDVFRKNIINDQNLYELIKGMFHSQLFNENKSQIKSLIKALLSDLVSSHKLLEIIFGEFATDNLKNNNEFLKNLLIIQNKLTQTKSLDNIINVFVDALFDNDNEYLLQGPLSNVLAKIVKTNKALLTTRIDNLFNDLFKDQTTLDATYNILIRVLGQNIPDFIKTPQASLIIKKILSKGTNLKLINTLKLNTIDLLTDSKTYAGIFDSSETSIINLIQKSIDFTSKSFISDVLDLLTSDNVDLNE", "aa_hexdigest": "160f00f805eb32599c76ee4aab5774ed", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 31214.53200000006, "isoelectric_point": 5.329871940612793 }, "id": "NKIECMJPJD_1016", "locus": "GCA000828735_01012" }, { "type": "cds", "sequence": "contig_1", "start": 597694, "stop": 597786, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTCAAGATATTTTTCTAAAAATGTCTTTGGGACAAAATTACGCTCAGCAAAAAACTGCTTCTATTGCAAATGCTATTTATGATGCATGA", "aa": "MAQDIFLKMSLGQNYAQQKTASIANAIYDA", "aa_hexdigest": "7c0c0f0c73fd127b18de5a71ecfdb41b", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3305.7340999999988, "isoelectric_point": 5.71319408416748 }, "id": "NKIECMJPJD_1017", "locus": "GCA000828735_01013" }, { "type": "cds", "sequence": "contig_1", "start": 597856, "stop": 598464, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAGGTGCTGGAAGTGATGAGTATTTTAAAAATCAAGAATATAGATTTTTTGATTTTTCTAAACAAATGGATCAACAAAAAGATAATCCTTTTAAAAACCGTTTATCAAAGTTTTTTAAAAATTTTGGTTATGATTCAAAATTAACACCAAAAAATAATCATGAAGTTAATATTAATGATTTTAATGGTTTAAAAGAGCAAATTAAAAAAGCTAATTTAATGACAATCACCTTAGGTGCTAATGATTTTTTAAGATATTTAGACATTCCAAAACTTGTTAGTTTAACAAATTTGAAAGGTAATGATTTAGAAATAAAAGTTAAAGAGTTCGAAAATAATTTACAAAAGGCATCTTTAGAAATTACAAAAAATCTAAAGAAATTAATCGCTCTTATTCGTACTTTAAATTCTAATGTATCTATTACATTAGTAAGTTATCCATTACCACTATTACGTTTACAAGATGTAATAGATAGTTCATTTAAATTAACAAATAAACGTCGTTTAAGTGATGAAATTATAAATATTCTAAATAGTGCCATTAAAAAAACATATGATTTAAATAATAATATCAACTATATCGATGCTTTTGATGAGAATGATTGA", "aa": "MLGAGSDEYFKNQEYRFFDFSKQMDQQKDNPFKNRLSKFFKNFGYDSKLTPKNNHEVNINDFNGLKEQIKKANLMTITLGANDFLRYLDIPKLVSLTNLKGNDLEIKVKEFENNLQKASLEITKNLKKLIALIRTLNSNVSITLVSYPLPLLRLQDVIDSSFKLTNKRRLSDEIINILNSAIKKTYDLNNNINYIDAFDEND", "aa_hexdigest": "c439ab567e644410200aabe80109295b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 23469.51950000003, "isoelectric_point": 9.046155357360838 }, "id": "NKIECMJPJD_1018", "locus": "GCA000828735_01014" }, { "type": "cds", "sequence": "contig_1", "start": 598621, "stop": 598941, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATAAAAAAAGTAAGAAAATTCTAGGATTAAGTGTTGCTTCAATTATTGCAGCACTTGCGATTATTGCTCCATCTACTTATTTTGGTTTACAAAAACAAGAAGAGGGTAAAAAAGATATTAATTTAGATAATGTTACGCGAAAAATACCTGGTGAAACTAAAGATGATATCAAAGTTGAAGATTTAAATAATATTAATGATCAAAATACAGATCTTAAACTTGATTTTAGTAAGGTAAGTATTAAACCCCAAACAAAAATTAATTATTTAGCGATTGGTGATTCAATCACAGCTGGTTTTAATTCAGAATTAGGTTGA", "aa": "MDKKSKKILGLSVASIIAALAIIAPSTYFGLQKQEEGKKDINLDNVTRKIPGETKDDIKVEDLNNINDQNTDLKLDFSKVSIKPQTKINYLAIGDSITAGFNSELG", "aa_hexdigest": "4ece85276de056647b6e7f70ef1ccd23", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11580.099199999986, "isoelectric_point": 6.232304573059082 }, "id": "NKIECMJPJD_1019", "locus": "GCA000828735_01015" }, { "type": "cds", "sequence": "contig_1", "start": 599758, "stop": 600348, "strand": "+", "frame": 1, "gene": "acrR", "product": "TetR/AcrR family transcriptional regulator", "db_xrefs": [ "COG:COG1309", "COG:K", "RefSeq:WP_006688876.1", "SO:0001217", "UniParc:UPI00000C1C03", "UniRef:UniRef100_A0AAC9T0X1", "UniRef:UniRef50_A0AAC9T0X1", "UniRef:UniRef90_A0AAC9T0X1" ], "nt": "ATGGCAGTAGAAAAAATTAAAATTTCAAAAGTTAAATTAAAAATTGTTTCAGCTTTATTTAATTTGTTGAAAACCAATTCATTTAACAAAATTAAAATTTCACAAATTATTGAGGTTGCAGATGTTTCGCGACCTTCATATTATCGTAATTTTAGTGATAAAATTTCGATTATTAATTATTTTATTAAAAATATTTATAATTGGAAAGTTGTAAGTAAATTAATAAATAATAACCTAAATATCAATTTTGATGAAGTTGGTTTTACTAAATTTTATGAAATTTCATTACAAATTAGTTTAGAATTCAAAGAATATTTTCTAATCTTAGATAAAAATAATTTTAGTGGATTAATTGTTGATTTAACCACTGAAAGTGGTTTTTATGCTATAGGTAATATGTCAAATAATTCAATTGATAAATATTTAATTAATTATTTAACAGGATTTTTTTGTAATATTAGAATGCAATGGTTAAAAAATGGTGCTAAAGAATCAATTCATGATATGGCGGTATTTTTAACTAACGTTTTAAAAGATAATGTTATTAAAAAATTAGAGAAATATCAATCTTGTAATAATATTAATGATTAA", "aa": "MAVEKIKISKVKLKIVSALFNLLKTNSFNKIKISQIIEVADVSRPSYYRNFSDKISIINYFIKNIYNWKVVSKLINNNLNINFDEVGFTKFYEISLQISLEFKEYFLILDKNNFSGLIVDLTTESGFYAIGNMSNNSIDKYLINYLTGFFCNIRMQWLKNGAKESIHDMAVFLTNVLKDNVIKKLEKYQSCNNIND", "aa_hexdigest": "4dca5e4ac4ed570c10b5622f4da132ff", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00000C1C03", "ncbi_nrp_id": "WP_006688876.1", "uniref100_id": "UniRef100_A0AAC9T0X1", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1C03", "RefSeq:WP_006688876.1", "UniRef:UniRef100_A0AAC9T0X1" ] }, "ips": { "uniref100_id": "UniRef100_A0AAC9T0X1", "uniref90_id": "UniRef90_A0AAC9T0X1" }, "psc": { "uniref90_id": "UniRef90_A0AAC9T0X1", "gene": "AcrR", "product": "TetR/AcrR family transcriptional regulator", "uniref50_id": "UniRef50_A0AAC9T0X1", "cog_id": "COG1309", "cog_category": "K" }, "pscc": { "uniref50_id": "UniRef50_A0AAC9T0X1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T0X1" ], "product": "TetR/AcrR family transcriptional regulator" }, "genes": [ "acrR" ], "id": "NKIECMJPJD_1020", "locus": "GCA000828735_01016" }, { "type": "cds", "sequence": "contig_1", "start": 600384, "stop": 600581, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01625.27" ], "nt": "GTGGATCACGATGATGAGTTTATTATTAAAGATTTTTTATTAAAAAAACAAAATCAAACACCAAAAAAAATTATGATTGAAGTTGAAAGATTACGCAATTTTAATATTGCTGAAGAATACCATCAAAAATATTTAGATAAAAATCCTAATAGTTATTGTCATGTTGATTTATCATTATCTAAAAAAGAATTCAGATAA", "aa": "MDHDDEFIIKDFLLKKQNQTPKKIMIEVERLRNFNIAEEYHQKYLDKNPNSYCHVDLSLSKKEFR", "aa_hexdigest": "df7e592796ab79ca0fa5c2d0948b8546", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01625.27", "name": "PMSR", "length": 50, "aa_cov": 0.7384615384615385, "hmm_cov": 1.0, "evalue": 2.781748726744987e-12, "score": 42.70988082885742, "start": 7, "stop": 54 } ], "seq_stats": { "molecular_weight": 7986.0394, "isoelectric_point": 6.821895408630371 }, "id": "NKIECMJPJD_1021", "locus": "GCA000828735_01017" }, { "type": "cds", "sequence": "contig_1", "start": 600952, "stop": 601077, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACCGTTGCATATCATAATTTAGATATTAATGACCCTAAAAATGTTCTTTTAGCAACGCAAAAATTCTTTAATCATATTAATGAAATAATTATTGATTATGGTTTAGATAAACGTGATCAATGA", "aa": "MTVAYHNLDINDPKNVLLATQKFFNHINEIIIDYGLDKRDQ", "aa_hexdigest": "715b6b04eb3eaa8b3b20c6ac134d39a4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4831.419400000001, "isoelectric_point": 5.326916313171386 }, "id": "NKIECMJPJD_1022", "locus": "GCA000828735_01018" }, { "type": "cds", "sequence": "contig_1", "start": 601102, "stop": 601644, "strand": "-", "frame": 3, "gene": null, "product": "Unique hypothetical", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQK4", "UniRef:UniRef90_Q9PQK4" ], "nt": "TTGACTAATAATATTATTGTTGATAAAAATAACAAAAATATTTATCATCAAAACGACAAACAAATTTTTGATTTTACTTTATGTTTATCTGGTCTTTTGTCTAAAACTTTAGGTTTAAATCATAAATCATCTTCGCTTTATTTAACAAATGATGATGAAGTAGCGCATCGTTTTAAGATTAAAGTTTTGCATAATTATTTTAATTTATATAATATTGAAGAATTTTTTACGTTTATGGATAATCAAATTCCTTTAGAAAATGCAAAAAATATTGCACGTTTAAGTTTAATTTATGATTTAAAACAACATGAAAATATTTTAAAAACTATATCGAATGAAGGTTTAGAAAATATTGATTTAGAATTTGCTTTCATTAATTTTTTAAGAGCAAAAATCTCTATTAATTTTGCCTATAGTTATGATATTTCTGAAGTAATTTTATTGTTGCGAACAGGGCTAGATTTACATTTTTTAGAAAATGAGCATTTTAGTAATCTTATACCAGTTTTTGGATTAGAAACAATTAAATATTTCAAGAATTGA", "aa": "MTNNIIVDKNNKNIYHQNDKQIFDFTLCLSGLLSKTLGLNHKSSSLYLTNDDEVAHRFKIKVLHNYFNLYNIEEFFTFMDNQIPLENAKNIARLSLIYDLKQHENILKTISNEGLENIDLEFAFINFLRAKISINFAYSYDISEVILLLRTGLDLHFLENEHFSNLIPVFGLETIKYFKN", "aa_hexdigest": "ec7cd884b3f9a474a85a2d8be96a4f3f", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_Q9PQK4", "query_cov": 0.9055555555555556, "subject_cov": 0.8109452736318408, "identity": 0.963, "score": 311.0, "evalue": 3.75e-106, "valid": true, "product": "Unique hypothetical", "uniref50_id": "UniRef50_Q9PQK4" }, "pscc": { "uniref50_id": "UniRef50_Q9PQK4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQK4" ], "product": "Unique hypothetical" }, "genes": [], "id": "NKIECMJPJD_1023", "locus": "GCA000828735_01019" }, { "type": "cds", "sequence": "contig_1", "start": 601751, "stop": 602155, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00152.25" ], "nt": "ATGCCAACTTTAGAAACATTAAATACTTTTGATGTAAATAAAAAAGACGCTAGAGGTCGTAGTTATGATATTGTTTTAAATGGTTATGAATTAGGTGGAGGATCAGTTCGGATTATAGACCAACAAATTCAACGCCGTATGTTTAAATCAATTAATATGAGTGATGAAGAGGCTAATTTAAAATTCGGTTTTTTACTAACTGCATTCGAATATGGTGTTCCGCCACATTGTGGAATTGCTTTGGGTTTAGATCGTTTGATGATGATTTTAGTGAATAGTGAATATATTCGCGATGTTGTTGCATTTCCTAAGAATAACAACGGCGTTGATATGATGTTAGACGCACCATCTAACATGAATGATGAAGATTTAAAAGAATTAGGTTTAAAAATAAAAAATGATTAA", "aa": "MPTLETLNTFDVNKKDARGRSYDIVLNGYELGGGSVRIIDQQIQRRMFKSINMSDEEANLKFGFLLTAFEYGVPPHCGIALGLDRLMMILVNSEYIRDVVAFPKNNNGVDMMLDAPSNMNDEDLKELGLKIKND", "aa_hexdigest": "121088cd2772c7af93041e95067cf6f3", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0030EC2591", "query_cov": 0.8283582089552238, "subject_cov": 0.9487179487179487, "identity": 0.613, "score": 144.0, "evalue": 3.26e-42, "valid": false, "product": "amino acid--tRNA ligase-related protein", "uniref50_id": "UniRef50_A0A358XMQ2" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00152.25", "name": "tRNA-synt_2", "length": 92, "aa_cov": 0.6865671641791045, "hmm_cov": 1.0, "evalue": 4.136602847937085e-37, "score": 123.39860534667969, "start": 14, "stop": 105 } ], "seq_stats": { "molecular_weight": 15105.176499999989, "isoelectric_point": 4.8733980178833 }, "id": "NKIECMJPJD_1024", "locus": "GCA000828735_01020" }, { "type": "cds", "sequence": "contig_1", "start": 602450, "stop": 603403, "strand": "-", "frame": 2, "gene": "aspS", "product": "aspartate--tRNA ligase", "db_xrefs": [ "COG:COG0173", "COG:J", "EC:6.1.1.12", "GO:0000166", "GO:0003676", "GO:0004812", "GO:0004815", "GO:0005524", "GO:0005737", "GO:0006418", "GO:0006422", "GO:0016874", "SO:0001217", "UniRef:UniRef50_Q9PQK5", "UniRef:UniRef90_Q9PQK5" ], "nt": "ATGGGTAATTTAGTTTTTGTTGACCTAAAAGATCGATTTGGAATTGTACAAATTTTTGCAACAAAAAGTGATGGTGTTTTTAATGAATTAACACAACTTTCACGTGAGGATGTAATTAATGTTGAAGGTTTAGTTTTGTTGAGAAAAAACCCTAATAATGATTTAAAAACAGGTAGATTTGAAATTCATGTCAAAAAAATACTTATTTATTCAAAAGCAAAAACACCACCATTAATTATTGAAGATGAAACTGATGCAAATGAGGAAATTCGTTTTAGATATCGTTATTTAGATTTACGTCGTGATGTTAATTTAAAAATTTTTGAATTGCGAAGTAAAGTTTACCAAGCTTTTAGAAATTACTTATATTCACAAGACTTTATTGAAACTGAAACTCCTATTTTAGCAAAACCAACGCCAGAAGGTGCACGAGATTTTTATGTGCCAACAAGAACTAGAAAATTTTATGCTTTACCACAATCACCTCAAACTTTTAAACAATTATTAATGGTTGCGGGTTTTCAAAAATATTTTCAAATTACAAAATGTTTTCGTGATGAAGATTTAAGAAGCGATCGTCAACCAGAATTTACTCAAGTTGATATAGAATTATCATTTGCTGATGAAATAGAAATTCAGACATTAATTGAAAATTTATTAAAATATGTTTTTAAACAAACCATTAATGTTGATTTAACAACTCCTTTTATGCGAATGAGTTATGAGCAAGCGATAAATGATTATGGTAGTGATAAACCAGATTTACGTTTTGATTTGAAAATTAAAACACTTAACACATATTTTGAAAATTCAAAAACATTATTTTTTCAAAAAGCACTTTTAAATAATCAAAGTATTCGTGCAATTTTAGTACCTAATATTAATTTAAATAAAAAACAAGTACAAACTCTTGAAAAGTTTGCCAAAGACAAAGGTGCTAAAGGTTTAAGTTGA", "aa": "MGNLVFVDLKDRFGIVQIFATKSDGVFNELTQLSREDVINVEGLVLLRKNPNNDLKTGRFEIHVKKILIYSKAKTPPLIIEDETDANEEIRFRYRYLDLRRDVNLKIFELRSKVYQAFRNYLYSQDFIETETPILAKPTPEGARDFYVPTRTRKFYALPQSPQTFKQLLMVAGFQKYFQITKCFRDEDLRSDRQPEFTQVDIELSFADEIEIQTLIENLLKYVFKQTINVDLTTPFMRMSYEQAINDYGSDKPDLRFDLKIKTLNTYFENSKTLFFQKALLNNQSIRAILVPNINLNKKQVQTLEKFAKDKGAKGLS", "aa_hexdigest": "129d341a110d94677b533a1fb707cb0f", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A920AYN4", "query_cov": 0.8927444794952681, "subject_cov": 0.8397626112759644, "identity": 0.502, "score": 276.0, "evalue": 4.07e-88, "valid": false, "gene": "aspS", "product": "aspartate--tRNA ligase", "ec_ids": [ "6.1.1.12" ], "uniref50_id": "UniRef50_A0A3N5JT57", "go_ids": [ "GO:0000166", "GO:0004812", "GO:0005524", "GO:0006418", "GO:0016874" ] }, "genes": [ "aspS" ], "truncated": "both", "pseudogene": { "start": 602147, "stop": 603487, "observations": { "mutation": [ 602218, 602230, 602452, 603424 ], "stop": [ 602218, 602230, 602452, 603424 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQK5", "query_cov": 1.0, "subject_cov": 0.5484429065743944, "identity": 1.0, "score": 620.0, "evalue": 3.34e-220, "gene_start": 1, "gene_end": 317, "reference_start": 29, "reference_end": 345, "reference_sequence": "MKVYCGYIGEEHLEKNVILNGWVKKVRKMGNLVFVDLKDRFGIVQIFATKSDGVFNELTQLSREDVINVEGLVLLRKNPNNDLKTGRFEIHVKKILIYSKAKTPPLIIEDETDANEEIRFRYRYLDLRRDVNLKIFELRSKVYQAFRNYLYSQDFIETETPILAKPTPEGARDFYVPTRTRKFYALPQSPQTFKQLLMVAGFQKYFQITKCFRDEDLRSDRQPEFTQVDIELSFADEIEIQTLIENLLKYVFKQTINVDLTTPFMRMSYEQAINDYGSDKPDLRFDLKIKTLNTYFENSKTLFFQKALLNNQSIRAILVPNINLNKKQVQTLEKFAKDKGAKGLSWISIKDEKIIDGSLLSIQEDHIIYKTIFKDFNLSTGSILLVADTFDIASQALGLVRINLASILNLKKPNIFKFVWIIDWPLYEYDNEAQRFVAAHHPFTMPTLETLNTFDVNKKDARGRSYDIVLNGYELGGGSVRIIDQQIQRRMFKSINMSDEEANLKFGFLLTAFEYGVPPHCGIALGLDRLMMILVNSEYIRDVVAFPKNNNGVDMMLDAPSNMNDEDLKELGLKIKND" }, "paralog": false, "identity": 0.9910313901345291, "score": 865.0, "evalue": 2.2e-319, "description": "Internal stop codon at 602218, 602230, 602452, 603424. Nonsense mutation around 602218, 602230, 602452, 603424.", "psc": { "uniref90_id": "UniRef90_Q9PQK5", "gene": "aspS", "product": "aspartate--tRNA ligase", "ec_ids": [ "6.1.1.12" ], "uniref50_id": "UniRef50_Q9PQK5", "cog_id": "COG0173", "cog_category": "J", "go_ids": [ "GO:0000166", "GO:0003676", "GO:0004812", "GO:0004815", "GO:0005524", "GO:0005737", "GO:0006418", "GO:0006422", "GO:0016874" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQK5", "EC:6.1.1.12", "UniRef:UniRef50_Q9PQK5", "COG:COG0173", "COG:J", "GO:0000166", "GO:0003676", "GO:0004812", "GO:0004815", "GO:0005524", "GO:0005737", "GO:0006418", "GO:0006422", "GO:0016874" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQK5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQK5" ], "product": "Aspartate--tRNA ligase" } }, "id": "NKIECMJPJD_1025", "locus": "GCA000828735_01021" }, { "type": "cds", "sequence": "contig_1", "start": 603577, "stop": 604215, "strand": "-", "frame": 3, "gene": "hisS", "product": "histidine--tRNA ligase", "db_xrefs": [ "COG:COG0124", "COG:J", "EC:6.1.1.21", "GO:0004821", "GO:0005524", "GO:0005737", "GO:0006427", "KEGG:K01892", "SO:0001217", "UniRef:UniRef50_Q9PQK6", "UniRef:UniRef90_Q9PQK6" ], "nt": "TTGCAAGAATATTTTCATTTACATCGTACTGAATTAACTCCATTAAGTCAAGAACGTATTACAACCAATCCTTTGCGTATTTTAGATGATAAATTAGAATCTCAAAAACTAGTTGTAAAACAAGCTCCAAAAATTAGTCATTTTTTATCAAATGAAGAAAAAGAAGAATTTAATCTAATTAAAAAAATGCTTGATGATTATAACATTAAATATTATGTTAACGAAGGATTAGTAAGAGGTTTGGATTATTATAGTGGATTAGTATTTGAATTTATTTCAACTTCACCTAAATTATTAGGTCAATCAACAATTATAGGTGGAGGCCGATATGGTGAATTAATTAAACAAACAGGTGGCCCTAATTATGAAGGTATTGGTTTTGGTATAGGAATTGAACGTTTATTAATAGCTTTATCTGAAACCAATAAAAATATTTTAAACACTGATGATGATAAATATTTGATTGCATTTTTTGATAAAGAATTAGAGAATGAAGCTATAAAACTAACTCAAATATTACGTATCAATAATCAATTAAATGTTGACATTATCTTAAGTTCAACTAAAGCTGACAAAATTTTTAAGTTAGCACAACGTTTAAATGTAAAAAAGCTTATAATATTAGCTAAAAAAGAATGA", "aa": "MQEYFHLHRTELTPLSQERITTNPLRILDDKLESQKLVVKQAPKISHFLSNEEKEEFNLIKKMLDDYNIKYYVNEGLVRGLDYYSGLVFEFISTSPKLLGQSTIIGGGRYGELIKQTGGPNYEGIGFGIGIERLLIALSETNKNILNTDDDKYLIAFFDKELENEAIKLTQILRINNQLNVDIILSSTKADKIFKLAQRLNVKKLIILAKKE", "aa_hexdigest": "bde7990378c3056252f0deb41aee9653", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "hisS" ], "truncated": "both", "pseudogene": { "start": 603493, "stop": 604515, "observations": { "mutation": [ 603579, 604227 ], "stop": [ 603579, 604227 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQK6", "query_cov": 1.0, "subject_cov": 0.5047619047619047, "identity": 0.995, "score": 403.0, "evalue": 7.58e-139, "gene_start": 1, "gene_end": 212, "reference_start": 181, "reference_end": 392, "reference_sequence": "MINYSKPRGTIDLYNKEMNDFKLLENFLLSTTKRYGFQQIKTPVFEFAELFMKSAGESSDLVSKEMYLFKDKSDRWLALRPEGTAGVIRAVVENKLLLNHPLPLKLMYFEPCFRYERPQAGRQRQFHQFGVEVLGTKNIYYDFELIALADTILKKLMISNYILEINYISSPHNRSLWVKSLQEYFHLHRTELTPLSQERITTNPLRILDDKLESQKLVVKQAPKISHFLSNEEKEEFNLIKKMLDDYNIKYYVNEGLVRGLDYYSGLVFEFISTSPKLLGQSTIIGGGRYGELIKQTGGPNYEGIGFGIGIERLLIALSETNKNILNTDDDKYLIAFFDKELENEAIKLTQILRINNQLNVDIILSSTKADKIFKLAQRLNVKKLIILAKKEWSDKKIILKDLLNFKQDLLSLDEIKGIR" }, "paralog": false, "identity": 0.9941176470588236, "score": 659.0, "evalue": 2.78e-242, "description": "Internal stop codon at 603579, 604227. Nonsense mutation around 603579, 604227.", "psc": { "uniref90_id": "UniRef90_Q9PQK6", "gene": "hisS", "product": "histidine--tRNA ligase", "ec_ids": [ "6.1.1.21" ], "uniref50_id": "UniRef50_Q9PQK6", "cog_id": "COG0124", "cog_category": "J", "kegg_orthology_id": "K01892", "go_ids": [ "GO:0004821", "GO:0005524", "GO:0005737", "GO:0006427" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQK6", "EC:6.1.1.21", "UniRef:UniRef50_Q9PQK6", "COG:COG0124", "COG:J", "KEGG:K01892", "GO:0004821", "GO:0005524", "GO:0005737", "GO:0006427" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQK6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQK6" ], "product": "Histidine--tRNA ligase" } }, "id": "NKIECMJPJD_1026", "locus": "GCA000828735_01022" }, { "type": "cds", "sequence": "contig_1", "start": 604225, "stop": 604437, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00587.30", "PFAM:PF13393.11" ], "nt": "ATGTATTTTGAGCCTTGTTTTCGTTATGAACGCCCTCAAGCTGGACGTCAACGCCAATTTCATCAATTTGGTGTTGAAGTGTTGGGAACAAAAAATATTTATTATGATTTTGAATTAATTGCTTTAGCTGATACAATTTTAAAAAAATTAATGATATCTAATTATATTTTAGAAATTAATTATATCTCATCTCCTCATAATCGTTCATTATGA", "aa": "MYFEPCFRYERPQAGRQRQFHQFGVEVLGTKNIYYDFELIALADTILKKLMISNYILEINYISSPHNRSL", "aa_hexdigest": "c70dd34948599a594b39b60dc81c3712", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00587.30", "name": "tRNA-synt_2b", "length": 65, "aa_cov": 0.8428571428571429, "hmm_cov": 1.0, "evalue": 2.4693520219155806e-09, "score": 33.01798629760742, "start": 2, "stop": 60 }, { "id": "PF13393.11", "name": "tRNA-synt_His", "length": 61, "aa_cov": 0.8714285714285714, "hmm_cov": 1.0, "evalue": 7.202037237634432e-11, "score": 37.388572692871094, "start": 1, "stop": 61 } ], "seq_stats": { "molecular_weight": 8391.572999999993, "isoelectric_point": 7.888045310974121 }, "id": "NKIECMJPJD_1027", "locus": "GCA000828735_01023" }, { "type": "cds", "sequence": "contig_1", "start": 604528, "stop": 604755, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13393.11" ], "nt": "ATGATTAATTACAGTAAACCACGAGGTACTATAGATTTATATAATAAAGAAATGAATGATTTTAAATTACTTGAAAATTTTTTATTATCAACTACTAAAAGATATGGCTTTCAGCAAATTAAAACACCTGTTTTTGAATTTGCAGAACTCTTTATGAAATCAGCTGGTGAAAGTTCAGATTTAGTTTCAAAAGAAATGTATTTATTTAAAGATAAATCAGATCGCTGA", "aa": "MINYSKPRGTIDLYNKEMNDFKLLENFLLSTTKRYGFQQIKTPVFEFAELFMKSAGESSDLVSKEMYLFKDKSDR", "aa_hexdigest": "7d5457290739b05c43af2de906159c27", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13393.11", "name": "tRNA-synt_His", "length": 66, "aa_cov": 0.88, "hmm_cov": 0.9393939393939394, "evalue": 2.0394742150145175e-07, "score": 26.050662994384766, "start": 10, "stop": 75 } ], "seq_stats": { "molecular_weight": 8875.098699999997, "isoelectric_point": 7.925501441955567 }, "id": "NKIECMJPJD_1028", "locus": "GCA000828735_01024" }, { "type": "cds", "sequence": "contig_1", "start": 604759, "stop": 604911, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGATAATAATTATATTGATGTTTTAGAATACACAAAAAATCATTTTTATGTTTTAACAATTTTTAATCCAGAATATACATCAAAACCTTCAAAGGCAAATCCTTATTTTATTAATTTATTAAAGATGAGTTTAAAAATAAAAGAAAGTTAG", "aa": "MDNNYIDVLEYTKNHFYVLTIFNPEYTSKPSKANPYFINLLKMSLKIKES", "aa_hexdigest": "9509f6e245a01be79dbbb99aa40ea3b2", "start_type": "GTG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5976.825, "isoelectric_point": 7.983909797668457 }, "id": "NKIECMJPJD_1029", "locus": "GCA000828735_01025" }, { "type": "cds", "sequence": "contig_1", "start": 604930, "stop": 605214, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGGAACTGGCGTTAATATTGGTATTGGTGAATTCATTAATAATGTACTAAAACTTCCAATTGAATTTATTAATTTAGGCAATGGTGATTTTAGTTTTTTAAAAGACGCTTTCGTTAAAAATGAAATTGAAAATTATCGAATTGGAGAGTATTGTTCTAATATTCAAGCTAACACTATAACAAGCCAAATTTATCTCAAACAAAATCAATTGAACGAGCGCCATCGTCATCATTTTGAATTTAACAATCATTATATTAATAATTATTTTTTAAATCAAAATTGA", "aa": "MGTGVNIGIGEFINNVLKLPIEFINLGNGDFSFLKDAFVKNEIENYRIGEYCSNIQANTITSQIYLKQNQLNERHRHHFEFNNHYINNYFLNQN", "aa_hexdigest": "63eb5e7a4d92ea276826962db6e577a4", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11027.180599999992, "isoelectric_point": 6.026490592956542 }, "id": "NKIECMJPJD_1030", "locus": "GCA000828735_01026" }, { "type": "cds", "sequence": "contig_1", "start": 605527, "stop": 605655, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTTTTGCTGCTTATGATGTTGAGTCAGTTTATTTATTACCAAATGTTTTGTTTGAGCAAGGGATTCATAAAACTATTTTAGATTTTTTTAGTCTACCTTTAAAAAATGATAATATTAATTCATGA", "aa": "MIFAAYDVESVYLLPNVLFEQGIHKTILDFFSLPLKNDNINS", "aa_hexdigest": "e0c56e8b4c446bccbc32e4de4f7fc0dc", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4830.5089, "isoelectric_point": 4.427268791198731 }, "id": "NKIECMJPJD_1031", "locus": "GCA000828735_01027" }, { "type": "cds", "sequence": "contig_1", "start": 605677, "stop": 606372, "strand": "-", "frame": 3, "gene": "pyrG", "product": "CTP synthase", "db_xrefs": [ "COG:COG0504", "COG:F", "EC:6.3.4.2", "GO:0003883", "GO:0004359", "GO:0005524", "GO:0005829", "GO:0006221", "GO:0006241", "GO:0019856", "GO:0042802", "GO:0044210", "GO:0046872", "KEGG:K01937", "SO:0001217", "UniRef:UniRef50_Q9PQK7", "UniRef:UniRef90_Q9PQK7" ], "nt": "ATGAAACATATACAAAAAACAAAATTTATTTTTGTAACTGGTGGTGTTTATTCATCATTAGGTAAAGGGGTTAGTGCTAGTAGTATTGGTCGAATTCTTGTTGAATTAGGATATAGTGTTGCAATGCAAAAGCTTGATCCATATTTAAATATTGATCCCACTTATTTATCACCATTACAACATGGAGAAGTATTTGTTACAAAAGACGGTAAAGAAGCAGATTTAGATTTAGGTACTTATGAACGTTTTATTAATGCTGATTTAAATAAATATGCGTCAGTAACTTCAGGAAAAATTTATTATGAAATTTTAACAAAAGAGCGTGAAAATGGTTTTGACGGTAAAACTGTTCAAACTATTCCACATGTCACATCTGCAGTTATTGATTACATTAAAAAAATTAAAGATAGTTTAAAAACTGATTTTATTATTGTTGAAATTGGAGGAACAATTGGAGATATTGAATCGCTTCCATTTATTGAAGCTATTAGTCAATTTAAAACAATTTATGGTGTAAATAATGTAATGTTTATTCACTGTTCACCGCTTATTTATATTGAAAAAGTAGGTGAATTAAAAACAAAACCAACTCAACATAGCGTTAAGACGTTACGTTCATTAGGAATTAATTTAGATTTATTGTTATTAAGAACTAATCAAAAATTAGATGAAGTAACGATTAAAAAATTAGCATGA", "aa": "MKHIQKTKFIFVTGGVYSSLGKGVSASSIGRILVELGYSVAMQKLDPYLNIDPTYLSPLQHGEVFVTKDGKEADLDLGTYERFINADLNKYASVTSGKIYYEILTKERENGFDGKTVQTIPHVTSAVIDYIKKIKDSLKTDFIIVEIGGTIGDIESLPFIEAISQFKTIYGVNNVMFIHCSPLIYIEKVGELKTKPTQHSVKTLRSLGINLDLLLLRTNQKLDEVTIKKLA", "aa_hexdigest": "a155d0e609022eab56c885fec284f8f7", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_UPI000382712F", "query_cov": 0.974025974025974, "subject_cov": 0.8211678832116789, "identity": 0.573, "score": 261.0, "evalue": 1.89e-84, "valid": false, "product": "CTP synthase", "ec_ids": [ "6.3.4.2" ], "uniref50_id": "UniRef50_A0A381VMU8", "kegg_orthology_id": "K01937", "go_ids": [ "GO:0003883", "GO:0006221", "GO:0006241" ] }, "genes": [ "pyrG" ], "truncated": "5-prime", "pseudogene": { "start": 605374, "stop": 606387, "observations": { "mutation": [ 605529, 605679 ], "stop": [ 605529, 605679 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQK7", "query_cov": 1.0, "subject_cov": 0.4261992619926199, "identity": 1.0, "score": 452.0, "evalue": 6.9e-156, "gene_start": 1, "gene_end": 231, "reference_start": 6, "reference_end": 236, "reference_sequence": "MEIDLMKHIQKTKFIFVTGGVYSSLGKGVSASSIGRILVELGYSVAMQKLDPYLNIDPTYLSPLQHGEVFVTKDGKEADLDLGTYERFINADLNKYASVTSGKIYYEILTKERENGFDGKTVQTIPHVTSAVIDYIKKIKDSLKTDFIIVEIGGTIGDIESLPFIEAISQFKTIYGVNNVMFIHCSPLIYIEKVGELKTKPTQHSVKTLRSLGINLDLLLLRTNQKLDEVTIKKLAWSCGLDIDMIFAAYDVESVYLLPNVLFEQGIHKTILDFFSLPLKNDNINSWIDFTDKITTFKKHNLVIGLVGKYVELPDAYKSVLASLELAAIELNIDLKIKYIQPQNLNENNINEELKKINGIVIPSIAGSIKGWPGALLAASYARKNNIPFLAVGTGVNIGIGEFINNVLKLPIEFINLGNGDFSFLKDAFVKNEIENYRIGEYCSNIQANTITSQIYLKQNQLNERHRHHFEFNNHYINNYFLNQNWKIGAISVDNNYIDVLEYTKNHFYVLTIFNPEYTSKPSKANPYFINLLKMSLKIKES" }, "paralog": false, "identity": 0.9940652818991098, "score": 652.0, "evalue": 2.69e-237, "description": "Internal stop codon at 605529, 605679. Nonsense mutation around 605529, 605679.", "psc": { "uniref90_id": "UniRef90_Q9PQK7", "gene": "pyrG", "product": "CTP synthase", "ec_ids": [ "6.3.4.2" ], "uniref50_id": "UniRef50_Q9PQK7", "cog_id": "COG0504", "cog_category": "F", "kegg_orthology_id": "K01937", "go_ids": [ "GO:0003883", "GO:0004359", "GO:0005524", "GO:0005829", "GO:0006221", "GO:0006241", "GO:0019856", "GO:0042802", "GO:0044210", "GO:0046872" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQK7", "EC:6.3.4.2", "UniRef:UniRef50_Q9PQK7", "COG:COG0504", "COG:F", "KEGG:K01937", "GO:0003883", "GO:0004359", "GO:0005524", "GO:0005829", "GO:0006221", "GO:0006241", "GO:0019856", "GO:0042802", "GO:0044210", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQK7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQK7" ], "product": "Putative CTP synthase" } }, "id": "NKIECMJPJD_1032", "locus": "GCA000828735_01028" }, { "type": "cds", "sequence": "contig_1", "start": 606372, "stop": 606530, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGCAAATATTTCAAAAGTTGATATTAGTAAAAATAAGACTGCACGCACTCATGATACTTTTTTAAAAATTTATGTAAAAAACTTAGATCACTTACAAAAGATTATTTTAGAACTTCAAGCTAAAAATGTTATTAATGAATGGAGATTGATCTAA", "aa": "MKANISKVDISKNKTARTHDTFLKIYVKNLDHLQKIILELQAKNVINEWRLI", "aa_hexdigest": "09193856b3603bc7d5dae58958366b33", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6147.199999999998, "isoelectric_point": 9.90029697418213 }, "id": "NKIECMJPJD_1033", "locus": "GCA000828735_01029" }, { "type": "cds", "sequence": "contig_1", "start": 606639, "stop": 606791, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTATTTTACAGATTTTGAAATTTCGCCTTGTTGTTCTAAAATTCCTGGAATTAATTGTGTTGCTAAAATTGTTCGCAATAAAGTAGAAATTCACCGCAATGATTGTTCTAAAGTTAAAAATTCTCGCTCAAAAACAATTGTAATTGAATGA", "aa": "MYFTDFEISPCCSKIPGINCVAKIVRNKVEIHRNDCSKVKNSRSKTIVIE", "aa_hexdigest": "604153c230d2d81866b10177baebab30", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5715.673200000001, "isoelectric_point": 9.098245811462402 }, "id": "NKIECMJPJD_1034", "locus": "GCA000828735_01030" }, { "type": "cds", "sequence": "contig_1", "start": 606834, "stop": 607010, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTGAAGAGCATGCTATTAAAAGACTAGAAAAAGAATTTCAAGTTAATAGTATCAATACTTTTTTAGATAATATTGCTAATACTGACATTGATTTACAAACAAAATATAAAGTATTTTCAGAAAATAAACAAGTTTCACGTGAAGCAATTAATACAATTAAAAATCAAGCATGA", "aa": "MSEEHAIKRLEKEFQVNSINTFLDNIANTDIDLQTKYKVFSENKQVSREAINTIKNQA", "aa_hexdigest": "003d09da262a08e63fea20867304e3cf", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6756.479400000002, "isoelectric_point": 5.69995059967041 }, "id": "NKIECMJPJD_1035", "locus": "GCA000828735_01031" }, { "type": "cds", "sequence": "contig_1", "start": 607255, "stop": 607542, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAAAATTATTTATATCGTATTTATATGCTACATCTAATAATGTTGAACCATTTGAAACAGTGATTCATTTATTAGAATTAAGAATTAGTACATCAAAAATAGTTCCGGTTGTGACATCTTTAATAGTTTTAATTCCTTTATCTGTGCTCAATAAAAAATCACGCAATAAAACATTATTAACTGTTAATAAAATTTCAGTTTTATTATCTTTTTGTTCTTTATATTTTCAGTGTGCTGCTAAACCATTATTAGCAAATAAATTCATTTTTGTTGTTCTGATTTGA", "aa": "MKKLFISYLYATSNNVEPFETVIHLLELRISTSKIVPVVTSLIVLIPLSVLNKKSRNKTLLTVNKISVLLSFCSLYFQCAAKPLLANKFIFVVLI", "aa_hexdigest": "e685aedb8bd2fd5975a59a0a04e48dd4", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10713.91509999999, "isoelectric_point": 9.885920524597168 }, "id": "NKIECMJPJD_1036", "locus": "GCA000828735_01032" }, { "type": "cds", "sequence": "contig_1", "start": 607857, "stop": 608330, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATGAAAGTACATTAGTAGCAGGTATGTTACACGATGTTTTAGAAGACACAAATTGTCCAGAAGAAGTTATGGAAAAAACTTTTGGTAAAGAAGTGACGCAACTTGTTTGTTTTGTAACAAAAGTATCTCTTTATTCAAAAAATCGACGTAATAAAGTTTCACACACCAATCTTGAAGAAAAGTATTCAATTCAAGTGTTTATGTCAATGACACAAGACATTCGTGCTATGATTATTAAAATTGCTGATCGTTATCATAATATGCAAACTATTCAATACTTACGTTTAGAAAAAGCGAAAAGAATTGCAACAGAAACACTAGAAATTTACGCAAATATTTCTGGAAGATTAGGGATGTATCGTGTTAAAACAGATTTATTAGATATGTCTTTTAAAATTTTAGACCCACAAAATTATCAATTAGTAAACGATTCAATTAATAAATTGGTTGAAAACAATCAAAAAAAATGA", "aa": "MDESTLVAGMLHDVLEDTNCPEEVMEKTFGKEVTQLVCFVTKVSLYSKNRRNKVSHTNLEEKYSIQVFMSMTQDIRAMIIKIADRYHNMQTIQYLRLEKAKRIATETLEIYANISGRLGMYRVKTDLLDMSFKILDPQNYQLVNDSINKLVENNQKK", "aa_hexdigest": "a0593bb604bb0db86bfd6bb43dd90272", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18333.016400000004, "isoelectric_point": 7.733365440368652 }, "id": "NKIECMJPJD_1037", "locus": "GCA000828735_01033" }, { "type": "cds", "sequence": "contig_1", "start": 608334, "stop": 608528, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGAATTAGTTAAAAAAAAATTAGATGATTTATATGATTTTCTTAAACAAAAAAAATATTCAAGCCAAACCATCGAATTAGTCCATAAATCATTTGATTTTGCAAACTATTATCATGGTGATCAAACAAGAAAGTCGGGAGAACCATATATCATTCATCCAATTGCAACTGTACGAATTTTAGCTTCATGA", "aa": "MNELVKKKLDDLYDFLKQKKYSSQTIELVHKSFDFANYYHGDQTRKSGEPYIIHPIATVRILAS", "aa_hexdigest": "6620b8e8205a79ee86a4a08fa74828ec", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7533.5297, "isoelectric_point": 8.927146720886231 }, "id": "NKIECMJPJD_1038", "locus": "GCA000828735_01034" }, { "type": "cds", "sequence": "contig_1", "start": 608628, "stop": 609020, "strand": "-", "frame": 1, "gene": "hup", "product": "DNA-binding protein HU", "db_xrefs": [ "COG:B", "COG:COG0776", "GO:0003677", "GO:0030261", "GO:0030527", "RefSeq:WP_006688857.1", "SO:0001217", "UniParc:UPI0000128F24", "UniRef:UniRef100_Q9PQK9", "UniRef:UniRef50_Q9PQK9", "UniRef:UniRef90_Q9PQK9" ], "nt": "ATGAATGCAAAAATTAAAGCCAAAACACGTTCACAAATGATAGATGAATTATCAAAAATGTTAAATATTGAAAAAAAACAAACAAAAGCATTTATGGATACTTACGAAGCTTTTTTAATTCTTGAATTATCACGAGCTAAAGAAGTTCGTTTAGGAAATATTGGAAAATTCAAGGTTAGTGTACGTGCAGAACGTAAGGGAATTAACCCTAAAACGGGTGAAACTGTAATTATTCCCGAGAAAACAATTCCTAAATTTACTTTTACTAAAGGAATTAAAGAAATTATTAATGCTGGTATTTCAATTGATAATGAAAGAGTATCGATTGATGATAATGATTTTGATGATGACGATGAATTTGTAGAAGAGTACATTGTATCAGAAAATAATTAA", "aa": "MNAKIKAKTRSQMIDELSKMLNIEKKQTKAFMDTYEAFLILELSRAKEVRLGNIGKFKVSVRAERKGINPKTGETVIIPEKTIPKFTFTKGIKEIINAGISIDNERVSIDDNDFDDDDEFVEEYIVSENN", "aa_hexdigest": "5a394bd5e270e9dae20f6c81205e0674", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000128F24", "ncbi_nrp_id": "WP_006688857.1", "uniref100_id": "UniRef100_Q9PQK9", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000128F24", "RefSeq:WP_006688857.1", "UniRef:UniRef100_Q9PQK9" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQK9", "uniref90_id": "UniRef90_Q9PQK9" }, "psc": { "uniref90_id": "UniRef90_Q9PQK9", "gene": "hup", "product": "DNA-binding protein HU", "uniref50_id": "UniRef50_Q9PQK9", "cog_id": "COG0776", "cog_category": "B", "go_ids": [ "GO:0003677", "GO:0030527", "GO:0030261" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQK9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQK9" ], "product": "DNA-binding protein HU" }, "genes": [ "hup" ], "id": "NKIECMJPJD_1039", "locus": "GCA000828735_01035" }, { "type": "cds", "sequence": "contig_1", "start": 609116, "stop": 609703, "strand": "-", "frame": 2, "gene": "deoD", "product": "purine-nucleoside phosphorylase", "db_xrefs": [ "COG:COG0813", "COG:F", "EC:2.4.2.1", "GO:0004731", "GO:0006139", "KEGG:K03784", "SO:0001217", "UniRef:UniRef50_P47295", "UniRef:UniRef90_Q9PQL0" ], "nt": "ATGCTATGCTATACAGGAACATATAAAGGCAAAAAAATATCAATTATGGGCCATGGTATGGGAATTCCTTCGATCGGAATTTATTCATATGAATTATATAAATTTTATGAAGTTGAAACAATTATTCGTGTTGGTTCAACTGGTTCATATAAAAAAGATTTGAATGTTAATGATGTGGTTTTAGTTGAAAAATCATATAGTGATTCTACATTCGCATCACTGATTGGTGCACAAGTTGGCGATGATAAGGTTTTATTACCATCAAGAGAAGTTAATAATTTAATTAAAAATACTGCGAAGGAATTAAATTTAGAATTATCATTAGCAACTTGTCATGCTTCTGATGTGTTTTATAATAATGATTTTCAAACATTAGATGAAATTATTCATCGAACTAAATCCGATTGTGTTGATATGGAATCATTTGGTTTATTCGCAAATGCACAAATTTTAAATAAAAATGCAGCAACTTTACTAACAGTTAGTGATTCATTGATTACAGGTGATGCACTATCACCCGCAGAGCGTGCTAATACTTTTAAAAAAATGGTAACATTAGCCTTAGAAAGCGCAATTAAATTACTTTAA", "aa": "MLCYTGTYKGKKISIMGHGMGIPSIGIYSYELYKFYEVETIIRVGSTGSYKKDLNVNDVVLVEKSYSDSTFASLIGAQVGDDKVLLPSREVNNLIKNTAKELNLELSLATCHASDVFYNNDFQTLDEIIHRTKSDCVDMESFGLFANAQILNKNAATLLTVSDSLITGDALSPAERANTFKKMVTLALESAIKLL", "aa_hexdigest": "03798f125b1aa80b3892e6150efcbc52", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_Q9PQL0", "query_cov": 1.0, "subject_cov": 0.8158995815899581, "identity": 0.9129999999999999, "score": 351.0, "evalue": 3.71e-121, "valid": true, "gene": "deoD", "product": "purine-nucleoside phosphorylase", "ec_ids": [ "2.4.2.1" ], "uniref50_id": "UniRef50_P47295", "cog_id": "COG0813", "cog_category": "F", "kegg_orthology_id": "K03784", "go_ids": [ "GO:0004731", "GO:0006139" ] }, "pscc": { "uniref50_id": "UniRef50_P47295", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47295" ], "product": "Purine nucleoside phosphorylase DeoD-type" }, "genes": [ "deoD" ], "id": "NKIECMJPJD_1040", "locus": "GCA000828735_01036" }, { "type": "cds", "sequence": "contig_1", "start": 609909, "stop": 611000, "strand": "-", "frame": 1, "gene": "frvX", "product": "Endo-1,4-beta-glucanase", "db_xrefs": [ "COG:COG1363", "COG:EG", "RefSeq:WP_006688850.1", "SO:0001217", "UniParc:UPI00000C1BFE", "UniRef:UniRef100_Q9PQL1", "UniRef:UniRef50_Q9PQL1", "UniRef:UniRef90_Q9PQL1" ], "nt": "ATGAGTAAATTTAACGAAAAAGAAATTAGAAATAAAGCAATTGAATATATGGAAATATATGGTATGTCACGTCATGAAGAACGTGTTGCTTCCAAACTAAAAGCATCATTAAAAGATGCGGGAGTTGATTATGAACGTGATAATTTAGGTTCAATTATTTTTAAAAAAACAAATTCAAATAAAGGACCTAAAATCTTAATTGCTACACACATGGATGAAGTTGGTTTTGTAGTTCAACAAATTCTTGATAATGGTCAATTACTATTATCAATGGTAGGTGGTGTATGGCCTAATATTGCTATTGGATCAGTAGCAAAAGTGTATGTTGATGAACAACGTCAATATACTGGTGTATTTGGCCATACTTCAATTCATATCTTAGAACCTGAAGCACGCAGTAAAGCAGTTCCAGTTAAAGAATTATTTGTTGATTGCGGTTTTAATTCAAAACAACAAGCTATTGATTTAGGAATTGAAATAGGTACGGAAGTTTATATGGAAGGACCAACTTTAAATTTTTATGATGAAAATTATATTGTTGGTAAAGCTGTTGATAATCGTGTAAGTGTTGCGGTTTTAGATTTATTAATACATAGTTTAAAGGACAAAGTAATTCCCAACCAAACTTATTTTGCTGCAACAGTGCAAGAAGAAGTTGGATTACGTGGCGCAAAAACAGTTGTTTCAAAAGTAAAACCTGATATTGGGATTGTTATTGATACAACAACATCACACGATACATACAAGTGTCCAGAGGGTGATACAAAATTAAATGATGGTGTATGTATTAGAATTAAAGATGGTGGAACTTTAGTTAATCCTAAATTAGTAAAATATTTTGAAGAATTAGCTATTAAACACAACATTCCTTTATATAAATATGTTGCACGAGGAGGCGGGACTGATGCTGAAGAATTGCAATATGGGCCTAATGGTGGAGTATTAACAATTGGTTTGTCTATCCCACAACGTTATTTACATGCTCCAATTGGCGTAGCAACGGTTAAAGATATAAAAGCAGCCTTTGACTTAGTACGTGAGTTTTTAGAAGTATTTGATGAAAACGAATTTGAAAAAGTAAAATTTAAATAA", "aa": "MSKFNEKEIRNKAIEYMEIYGMSRHEERVASKLKASLKDAGVDYERDNLGSIIFKKTNSNKGPKILIATHMDEVGFVVQQILDNGQLLLSMVGGVWPNIAIGSVAKVYVDEQRQYTGVFGHTSIHILEPEARSKAVPVKELFVDCGFNSKQQAIDLGIEIGTEVYMEGPTLNFYDENYIVGKAVDNRVSVAVLDLLIHSLKDKVIPNQTYFAATVQEEVGLRGAKTVVSKVKPDIGIVIDTTTSHDTYKCPEGDTKLNDGVCIRIKDGGTLVNPKLVKYFEELAIKHNIPLYKYVARGGGTDAEELQYGPNGGVLTIGLSIPQRYLHAPIGVATVKDIKAAFDLVREFLEVFDENEFEKVKFK", "aa_hexdigest": "38ee7e2c4247905f8c6aabd5608bceee", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI00000C1BFE", "ncbi_nrp_id": "WP_006688850.1", "uniref100_id": "UniRef100_Q9PQL1", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1BFE", "RefSeq:WP_006688850.1", "UniRef:UniRef100_Q9PQL1" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQL1", "uniref90_id": "UniRef90_Q9PQL1" }, "psc": { "uniref90_id": "UniRef90_Q9PQL1", "gene": "FrvX", "product": "Endo-1,4-beta-glucanase", "uniref50_id": "UniRef50_Q9PQL1", "cog_id": "COG1363", "cog_category": "EG" }, "pscc": { "uniref50_id": "UniRef50_Q9PQL1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQL1" ], "product": "Endo-1,4-beta-glucanase" }, "genes": [ "frvX" ], "id": "NKIECMJPJD_1041", "locus": "GCA000828735_01037" }, { "type": "cds", "sequence": "contig_1", "start": 611004, "stop": 611807, "strand": "-", "frame": 1, "gene": "pgk", "product": "Phosphoglycerate kinase", "db_xrefs": [ "COG:COG0126", "COG:G", "EC:2.7.2.3", "GO:0004618", "GO:0005524", "GO:0005829", "GO:0006094", "GO:0006096", "GO:0043531", "KEGG:K00927", "SO:0001217", "UniRef:UniRef50_Q9PQL2", "UniRef:UniRef90_Q9PQL2" ], "nt": "ATGCTAGGGGATATTTTTATTGATGATGCATATGGAGTTGCTCATCGACAATTATCATCAAATTATCAAACAGCTAAATTTGCTAAAAAAAGTGCATTAGGTTTTTTAATTGTTAATGAAATTAACCATCTAGATATTGCTTTAGAAGTTCCTAAAAGTCCGTATTTAGCTTTAATAGGTGGTAACCGTGTTGCTGATAAAATTGCAGCAATTGAAGTATTATGTGAACGGGCTGATCAAGTTATTATTGGGGGAGGATTAGTCTATACATTTTTATATGCTCAAGGGTATAACGTTGGTTTAAATTTAGTAGAACGCAATATGATTGATGATTGCAATAATATATTAGAAAAATACGGTAAAAAAATTTTAATTTGTTTTGATTTTTTATGTAACAATGATTTTAGTGATACAAGACCGATTTATCGTAAAATAGATGAAGGTCTTGAAGGGTTATATGGTTTAGATATTGGTAAAAGAAGTTTAAAATTTATTAAACATGAAATTTATCGTTCTAAAACTATTTTACTAAATGGTCCCTTTGGTGTTATTGAAAATATTAAGAATTATGCACAAGGTACTACTGAAATATGTAAATCAATGGCAAAACAAACAACACGTGGCGCTTATACAATTATATGTGATATCGATACTTCGAGTCATGCTGAATACCTAGGATTAGATAAATATATTAATTTTATATCTACTGGTGGGGGCGCTAGCTTATCATATATTGAAGAAGGAGTTCTTGATGGTCTTGAAACCATTGATAATGTTCCTTTAAATGTTTTAAAGAAGGAGTAA", "aa": "MLGDIFIDDAYGVAHRQLSSNYQTAKFAKKSALGFLIVNEINHLDIALEVPKSPYLALIGGNRVADKIAAIEVLCERADQVIIGGGLVYTFLYAQGYNVGLNLVERNMIDDCNNILEKYGKKILICFDFLCNNDFSDTRPIYRKIDEGLEGLYGLDIGKRSLKFIKHEIYRSKTILLNGPFGVIENIKNYAQGTTEICKSMAKQTTRGAYTIICDIDTSSHAEYLGLDKYINFISTGGGASLSYIEEGVLDGLETIDNVPLNVLKKE", "aa_hexdigest": "aa69749a5943d1d27c2325c9aaf0e371", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "genes": [ "pgk" ], "truncated": "3-prime", "pseudogene": { "start": 611004, "stop": 612107, "observations": { "mutation": [ 611843 ], "stop": [ 611843 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQL2", "query_cov": 1.0, "subject_cov": 0.6327014218009479, "identity": 1.0, "score": 536.0, "evalue": 2.59e-190, "gene_start": 1, "gene_end": 267, "reference_start": 156, "reference_end": 422, "reference_sequence": "MNSLNKKSIKDLDVNGKTIVLHLDLNVVVDYENKRILNDRKLRASLPTINYLINHNAKIVILSHLGRIKTLADKQSGKYSLEIIVDELRNRVSKNVNRVVFSPLNYGETVVQMVNDLEERDILILENTRYCDISDEGEYVGLEWDGSEILGQFWGMLGDIFIDDAYGVAHRQLSSNYQTAKFAKKSALGFLIVNEINHLDIALEVPKSPYLALIGGNRVADKIAAIEVLCERADQVIIGGGLVYTFLYAQGYNVGLNLVERNMIDDCNNILEKYGKKILICFDFLCNNDFSDTRPIYRKIDEGLEGLYGLDIGKRSLKFIKHEIYRSKTILLNGPFGVIENIKNYAQGTTEICKSMAKQTTRGAYTIICDIDTSSHAEYLGLDKYINFISTGGGASLSYIEEGVLDGLETIDNVPLNVLKKE" }, "paralog": false, "identity": 0.997275204359673, "score": 728.0, "evalue": 2.92e-268, "description": "Internal stop codon at 611843. Nonsense mutation around 611843.", "psc": { "uniref90_id": "UniRef90_Q9PQL2", "gene": "pgk", "product": "Phosphoglycerate kinase", "ec_ids": [ "2.7.2.3" ], "uniref50_id": "UniRef50_Q9PQL2", "cog_id": "COG0126", "cog_category": "G", "kegg_orthology_id": "K00927", "go_ids": [ "GO:0005829", "GO:0043531", "GO:0005524", "GO:0004618", "GO:0006094", "GO:0006096" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQL2", "EC:2.7.2.3", "UniRef:UniRef50_Q9PQL2", "COG:COG0126", "COG:G", "KEGG:K00927", "GO:0005829", "GO:0043531", "GO:0005524", "GO:0004618", "GO:0006094", "GO:0006096" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQL2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQL2" ], "product": "Phosphoglycerate kinase" } }, "id": "NKIECMJPJD_1042", "locus": "GCA000828735_01038" }, { "type": "cds", "sequence": "contig_1", "start": 611841, "stop": 612272, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00162.24" ], "nt": "ATGAATAGTTTAAATAAAAAAAGTATAAAAGATCTTGATGTAAATGGTAAAACAATAGTTTTACATTTAGATTTAAATGTTGTTGTTGATTATGAAAATAAACGCATATTAAATGATCGTAAATTACGAGCATCACTACCAACTATTAATTATTTAATTAATCATAACGCTAAAATTGTTATTTTAAGTCATTTAGGTCGAATTAAAACCTTAGCAGATAAACAAAGTGGTAAATATAGTTTAGAAATTATTGTTGATGAACTCCGTAATCGTGTATCCAAGAATGTTAATCGTGTTGTATTTTCACCTTTAAATTATGGAGAAACGGTCGTACAAATGGTTAATGATTTAGAAGAAAGAGACATTTTAATTTTAGAAAATACGCGTTATTGTGATATTAGTGATGAAGGTGAATATGTTGGGCTTGAATGA", "aa": "MNSLNKKSIKDLDVNGKTIVLHLDLNVVVDYENKRILNDRKLRASLPTINYLINHNAKIVILSHLGRIKTLADKQSGKYSLEIIVDELRNRVSKNVNRVVFSPLNYGETVVQMVNDLEERDILILENTRYCDISDEGEYVGLE", "aa_hexdigest": "b6590c72885b818332a669a70c6b8e40", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00162.24", "name": "PGK", "length": 124, "aa_cov": 0.8671328671328671, "hmm_cov": 0.9354838709677419, "evalue": 1.3402538203274495e-29, "score": 98.84573364257812, "start": 9, "stop": 132 } ], "seq_stats": { "molecular_weight": 16408.64179999999, "isoelectric_point": 6.311708641052246 }, "id": "NKIECMJPJD_1043", "locus": "GCA000828735_01039" }, { "type": "cds", "sequence": "contig_1", "start": 612344, "stop": 612694, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF05746.21" ], "nt": "ATGAATTCGCATGTTATTATTGATGTAGATATTGCCTTATCAAAAAATAACAATAATCCTTTATATTATGTTCAATACGCACATGCTCGTGCCAATCAAGTATTAAATAAACAAGTTTATGAATTAGATTTTAAAACTGATCTATTAACAGAAACACGTGAACGTGAATTGTTGAATCAACTTCATTTCTATAAACAAACAATTGCTAATGCTGCAAATAATCGTGAACCACACCGAATTAGTAATTATTTATATGACTTAGCTCAAATTTTTCATAATTATTATGCAAATGTTAAAATTAATAATGATAATAATAAAGTTCTATCAGCACAACGTTATACATTAGTATGA", "aa": "MNSHVIIDVDIALSKNNNNPLYYVQYAHARANQVLNKQVYELDFKTDLLTETRERELLNQLHFYKQTIANAANNREPHRISNYLYDLAQIFHNYYANVKINNDNNKVLSAQRYTLV", "aa_hexdigest": "39b3d7ab009f7186e5d84049e1b58896", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF05746.21", "name": "DALR_1", "length": 96, "aa_cov": 0.8017241379310345, "hmm_cov": 0.9895833333333334, "evalue": 1.0694092139831817e-24, "score": 82.39945220947266, "start": 24, "stop": 116 } ], "seq_stats": { "molecular_weight": 13716.192899999978, "isoelectric_point": 7.971789741516113 }, "id": "NKIECMJPJD_1044", "locus": "GCA000828735_01040" }, { "type": "cds", "sequence": "contig_1", "start": 612713, "stop": 612922, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTGGGGTACAGATCATTTAGGTTATATTGCGCGTTTAAAAGCGGCAATGAATGCTTTAGGTTATGATCCAAATAATTTAGAAATTGTTTGTGCTCAAGTAATGAAATTAGTTAAAAATAATCAAGAATTTAAACTTTCAAAAAGGAGCGGACAATCATTAACAATCAAGGATTTAGTTGAAATTATTGGTAAAGATGCATTACGTTGA", "aa": "MWGTDHLGYIARLKAAMNALGYDPNNLEIVCAQVMKLVKNNQEFKLSKRSGQSLTIKDLVEIIGKDALR", "aa_hexdigest": "8262bf216fe6cdf5c768110ad09c76f8", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7732.980199999996, "isoelectric_point": 9.250649070739744 }, "id": "NKIECMJPJD_1045", "locus": "GCA000828735_01041" }, { "type": "cds", "sequence": "contig_1", "start": 613136, "stop": 613414, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAGTGTTAGTTCGTTATTTACAATTACAAAATATTAATATTCAATTACCAGCAGATGCCTACCATGGGCAAGAAATTCATTTAGTTGCACAAACTTTATACCAAACTTATAAAAATCAATTTATTAATGTACGTTTAAATGAAAAATATGAAATTGATGATGATATTGCTAATCAAGAAATTAAAAATTTTGCAGTTAAATACTTATTAAATGAAATTAAAAACGATTTAGCATCAATTAACACATTCATTGATACTTATACTTCAGAAAATTGA", "aa": "MSVLVRYLQLQNINIQLPADAYHGQEIHLVAQTLYQTYKNQFINVRLNEKYEIDDDIANQEIKNFAVKYLLNEIKNDLASINTFIDTYTSEN", "aa_hexdigest": "2b88246dbe031bf8b54049773c6e2e9c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10812.019499999988, "isoelectric_point": 4.7395421981811525 }, "id": "NKIECMJPJD_1046", "locus": "GCA000828735_01042" }, { "type": "cds", "sequence": "contig_1", "start": 613451, "stop": 613927, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03485.21" ], "nt": "ATGATTACACAAAAAATAAGTGAAGAATTAAATAAGGCTTTGGCGAAAATGGGTATTCATGATACACAGGAGACAAAGATTCTCGTTGATAAAACTAAAAATATTAAATTTGGTGATTTTTATACTAACATAGCAATGATTTTAAGTAAAAAAAATAATAAGAGTTCTTTAGAAATAGCTAAAGAAATAGCTAATAATTTTGAACAAGATTTGTTTTTAGAAGTTAATTTGCAGCCACCTGGATTTTTAAATTTTAAATTAAAAGCAAAAGATCATGAAAATTTATTGAAACAAATTTATTATGAAAAAGATAGATTTGGTCAATTCTCTAAAAAGAATATCACTTATAATATTGAATATGTTTCAGCTAACCCCACAGGTTATTTGCATATTGCTCACGCTGCTAATGCTATTTATGGTGATATTTTAGCTAATTTATTAAAAATTTATGGATATGATGTAGAAACTGAATATTGA", "aa": "MITQKISEELNKALAKMGIHDTQETKILVDKTKNIKFGDFYTNIAMILSKKNNKSSLEIAKEIANNFEQDLFLEVNLQPPGFLNFKLKAKDHENLLKQIYYEKDRFGQFSKKNITYNIEYVSANPTGYLHIAHAANAIYGDILANLLKIYGYDVETEY", "aa_hexdigest": "338b6f4d80b9925d95ad81c17031024b", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03485.21", "name": "Arg_tRNA_synt_N", "length": 84, "aa_cov": 0.5253164556962026, "hmm_cov": 1.0, "evalue": 2.6321179984123703e-22, "score": 74.84034729003906, "start": 5, "stop": 87 } ], "seq_stats": { "molecular_weight": 18203.625399999997, "isoelectric_point": 6.910962104797363 }, "id": "NKIECMJPJD_1047", "locus": "GCA000828735_01043" }, { "type": "cds", "sequence": "contig_1", "start": 614051, "stop": 614218, "strand": "-", "frame": 2, "gene": "rpsU", "product": "30S ribosomal protein S21", "db_xrefs": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:1990904", "RefSeq:WP_006688933.1", "SO:0001217", "UniParc:UPI0000134E8E", "UniRef:UniRef100_Q9PQL4", "UniRef:UniRef50_Q9PQL4", "UniRef:UniRef90_Q9PQL4" ], "nt": "ATGTCAAGAGGTGTAAGCGTTGAAGGCGATTTAGAAAAGGCTCTTAAGAAATTTAAACGTATTTCTAATGAAACAAAAAAAGATTCTAAACGTCACGAATATTATTTAAGTCCAAGAATTCGGCGTAAAGAAAAAATTAAAGAGGCTAATAAATATCGTAGTTTCTAA", "aa": "MSRGVSVEGDLEKALKKFKRISNETKKDSKRHEYYLSPRIRRKEKIKEANKYRSF", "aa_hexdigest": "cb734cf92ba171b24d152225521f83b5", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000134E8E", "ncbi_nrp_id": "WP_006688933.1", "uniref100_id": "UniRef100_Q9PQL4", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000134E8E", "RefSeq:WP_006688933.1", "UniRef:UniRef100_Q9PQL4" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQL4", "uniref90_id": "UniRef90_Q9PQL4" }, "psc": { "uniref90_id": "UniRef90_Q9PQL4", "gene": "rpsU", "product": "30S ribosomal protein S21", "uniref50_id": "UniRef50_Q9PQL4", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQL4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQL4" ], "product": "Small ribosomal subunit protein bS21" }, "genes": [ "rpsU" ], "id": "NKIECMJPJD_1048", "locus": "GCA000828735_01044" }, { "type": "cds", "sequence": "contig_1", "start": 614294, "stop": 614953, "strand": "-", "frame": 2, "gene": "pncB", "product": "nicotinate phosphoribosyltransferase", "db_xrefs": [ "COG:COG1488", "COG:H", "EC:6.3.4.21", "KEGG:K00763", "SO:0001217", "UniRef:UniRef50_Q9PQL5", "UniRef:UniRef90_Q9PQL5" ], "nt": "ATGAGTGATCGTTCTGATGATTATAGTTTACAACCATATGATGGGTATGCTGCTGCTGTTGGTGGAATGCAATACTTTGTTACGCAAAAGCAGGTTGAATTTTTAAAAGATATTAATTATGAATGTAAGGTAATTGGCTCAATGCCACATGCTTTAATTCAACAAAACAATGGTCGGATTGATTTAGCATGTGAAATGTTTGCACAAACTTTTCCTAGCGATCCTTTAATTGCTGTTATTGATTATAATAACAATGTTTTAAATGATCTTGAACAACTACGATATATGTTTGATCGTTTGTTTGCAGTACGAATTGATACAGCTAAGGATTTAATTGATGAAAGTTTATTATCGACTTTTAATAATGTTGGTAATCGTGATTTACATGGTTGTAATCCTTATCTTATTGCTTTAGTACGTGAATATTTAGATAATAATGGAGGAAAACATATTAAAATAATTGCTTCAAGTGGGATTGATTTAAATGGTATTAAAAATTTTAATAAACACAATTCTGCAATTGATTTTTATGGAATAGGCACATATTTAAGTCACTTATCAGTTCACATCACTGCTGACTTGGTTTGTTTAGATAATGTTTATGGTGCTAAAGTTGGCCGTAAAATCGCTAAGAATTTTTCTGAAATGATTTTATATTAA", "aa": "MSDRSDDYSLQPYDGYAAAVGGMQYFVTQKQVEFLKDINYECKVIGSMPHALIQQNNGRIDLACEMFAQTFPSDPLIAVIDYNNNVLNDLEQLRYMFDRLFAVRIDTAKDLIDESLLSTFNNVGNRDLHGCNPYLIALVREYLDNNGGKHIKIIASSGIDLNGIKNFNKHNSAIDFYGIGTYLSHLSVHITADLVCLDNVYGAKVGRKIAKNFSEMILY", "aa_hexdigest": "981d7aa43626818ed1f8e49c18584514", "start_type": "ATG", "rbs_motif": null, "genes": [ "pncB" ], "truncated": "3-prime", "pseudogene": { "start": 614294, "stop": 615253, "observations": { "mutation": [ 615058 ], "stop": [ 615058 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQL5", "query_cov": 1.0, "subject_cov": 0.6033057851239669, "identity": 0.927, "score": 419.0, "evalue": 5.95e-146, "gene_start": 1, "gene_end": 219, "reference_start": 145, "reference_end": 363, "reference_sequence": "MGPVIPNTRLIDFKFDRDLLNKAYTSHYFIKTCKIIELHAPSHSVIMQFTHFSKTPIMVCGTSEVLALLEFCLSRKELKQLKIYYVPDGHVIKPKEALFAIEGPYEIFGWLENIIDSILARRSSVATNCYNVLNVINDEQKVIYMSDRSDDYSLQPYDGYAAAVGGMQYFVTQKQVEFLKDINYECKVMGSMPHALIQQNNGRVDLACEMFAQTFPNDPLIAVIDYNNNVLNDLEQLRYMFDRLYAVRIDTAKDLIDNSLLSTFDNVRNHDLHGCNPYLIDLVREYLDNNGGEHIKIIASSAIDLNSIKNFNKHNSAIDFYGIGTYLTHLSIHITADLVCLDNVYGAKVGRKIAKNFAEMTLY" }, "paralog": false, "identity": 0.9341692789968652, "score": 609.0, "evalue": 2.75e-223, "description": "Internal stop codon at 615058. Nonsense mutation around 615058.", "psc": { "uniref90_id": "UniRef90_Q9PQL5", "gene": "PncB", "product": "nicotinate phosphoribosyltransferase", "ec_ids": [ "6.3.4.21" ], "uniref50_id": "UniRef50_Q9PQL5", "cog_id": "COG1488", "cog_category": "H", "kegg_orthology_id": "K00763", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQL5", "EC:6.3.4.21", "UniRef:UniRef50_Q9PQL5", "COG:COG1488", "COG:H", "KEGG:K00763" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQL5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQL5" ], "product": "nicotinate phosphoribosyltransferase" } }, "id": "NKIECMJPJD_1049", "locus": "GCA000828735_01045" }, { "type": "cds", "sequence": "contig_1", "start": 615056, "stop": 615385, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02749.22" ], "nt": "ATGGGACCAATTATTCCAAATACACGTTTAATTGATTTTAAATTTGATCGAGACTTATTAAATAGTGCCTATACTGCTCATTATTTTATTAAAACATGCAAGATAATTGAATTGCATGCACCAAATCATTCAGTTATTATGCAATTTACACACTTTAGCAAAACACCGATCATGGTGTGTGGAACAAGTGAGGTTTTAGCATTATTAGAGTTTTGTTTATCTAAAAAAGAACTAAAACAATTAAAAATATATTATGTTCCAGATGGACATGTTATTAAACCAAAAGAAGCTTTATTTGTTATCGAAGGGCCATATGAAATTTTTGGCTGA", "aa": "MGPIIPNTRLIDFKFDRDLLNSAYTAHYFIKTCKIIELHAPNHSVIMQFTHFSKTPIMVCGTSEVLALLEFCLSKKELKQLKIYYVPDGHVIKPKEALFVIEGPYEIFG", "aa_hexdigest": "65b04fae2e61639a71f11b0188830785", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02749.22", "name": "QRPTase_N", "length": 67, "aa_cov": 0.6146788990825688, "hmm_cov": 0.8507462686567164, "evalue": 9.61849975516917e-10, "score": 34.18281555175781, "start": 42, "stop": 108 } ], "seq_stats": { "molecular_weight": 12516.666199999989, "isoelectric_point": 7.745301628112793 }, "id": "NKIECMJPJD_1050", "locus": "GCA000828735_01046" }, { "type": "cds", "sequence": "contig_1", "start": 615718, "stop": 615957, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAGGATTTAAAAGTTAGAATTATGGGATTAAAAAAAGAAAAAAAAGATCGTTCAACGATTCAATTATTATTTGGAACAATTAAAAAACGCAAAATTGCGAACTATTTAATTATTGCTTTTGCCTTTTTAACATTTGCCTTAGCAATTATAGGAAGTTTTGAAGCTATTGCATCAAAATATGCTATTGGCATTGGATCAGGGCTTTCATTATTTATTACGATTGCTTTTAGTGTTTGA", "aa": "MEDLKVRIMGLKKEKKDRSTIQLLFGTIKKRKIANYLIIAFAFLTFALAIIGSFEAIASKYAIGIGSGLSLFITIAFSV", "aa_hexdigest": "847ed7c86a561211980a86e4f7246acd", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8715.444199999994, "isoelectric_point": 10.073716926574708 }, "id": "NKIECMJPJD_1051", "locus": "GCA000828735_01047" }, { "type": "cds", "sequence": "contig_1", "start": 616113, "stop": 616325, "strand": "+", "frame": 3, "gene": null, "product": "DUF4044 domain-containing protein", "db_xrefs": [ "RefSeq:WP_006688920.1", "SO:0001217", "UniParc:UPI000165F8A8", "UniRef:UniRef100_A0AAC9T0W5", "UniRef:UniRef50_A0AAC9T0W5", "UniRef:UniRef90_A0AAC9T0W5" ], "nt": "ATGAGTACAAAAGCATTTAACCCAAAAGACCGTGTTACCAAAACTGTCGTTTTAAGCGATTTTGATCAGCATCAAATTTATTCAAAAAAACATAAAAAAATGGACTTTAAAAAACGCGTTATTTTATCGATTTTTCTAATAATATTAACTTGCGGAGCAATTGTTTTATTAGTTTTTGTTATTACTGAATTATTTAGATTATTAAGTGCATAA", "aa": "MSTKAFNPKDRVTKTVVLSDFDQHQIYSKKHKKMDFKKRVILSIFLIILTCGAIVLLVFVITELFRLLSA", "aa_hexdigest": "01334c390fd057ec20ee58c95c7257e7", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI000165F8A8", "ncbi_nrp_id": "WP_006688920.1", "uniref100_id": "UniRef100_A0AAC9T0W5", "db_xrefs": [ "SO:0001217", "UniParc:UPI000165F8A8", "RefSeq:WP_006688920.1", "UniRef:UniRef100_A0AAC9T0W5" ] }, "ips": { "uniref100_id": "UniRef100_A0AAC9T0W5", "uniref90_id": "UniRef90_A0AAC9T0W5" }, "psc": { "uniref90_id": "UniRef90_A0AAC9T0W5", "product": "DUF4044 domain-containing protein", "uniref50_id": "UniRef50_A0AAC9T0W5" }, "pscc": { "uniref50_id": "UniRef50_A0AAC9T0W5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T0W5" ], "product": "DUF4044 domain-containing protein" }, "genes": [], "id": "NKIECMJPJD_1052", "locus": "GCA000828735_01048" }, { "type": "cds", "sequence": "contig_1", "start": 616340, "stop": 617050, "strand": "+", "frame": 2, "gene": null, "product": "radical SAM protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E3EC27E", "UniRef:UniRef90_UPI001E3EC27E" ], "nt": "ATGATTTTTAACAAAAAAACTAAACATTTATACATTCATATTCCATTTTGTAACTATATTTGTATATTTTCTGATTTTAAACGTATTTTAAAAACTCCTCAAACAAAAAAATTTTTTAAGAATTTTTTAGAAAATATTAAAATGCACATTAATAATTTTAAAATAAAGCAATTTAAAACAATCTACTTAGGCGGAGGTGTTCCAAATTGTTTAAACAATCAAGAATTAGAAATTTTATTAAAAACAATTTCTCCTTATGTTGATGATAATTGCGAATATACCATTGAATGTAATCCTGAATTAATAAATCAAACTCAAATTAATCTCTTCAAAAAATACAAAATTAATCGCATTAGTCTTAACGTTCAATCAATGAATAATGATGTGTTAAAACAAATGAATTTTATTCATACAAATCAAGATAGTAAAAAAGCGATTAATTTATTACATAAAAACGGATTGTATAATGTGTCTTGTGATTTTTTATTTTGCATATCAATCTTAACATTAAAAGACTTAGATGATGTTTTTAATTTTATTTTAAATCATAAAATTAATCATGTTAGTTTTTATAGTTTAGAAATTAAAGAACAGTCACTTTTAAAAAAGTACCATATTACTATCGATGAAGAAAAAGAAATTGAACAAATGAATTATATAATTAATAAATTTGCAGGATTAAATTTTCATCGTTATGAAGTATGTAATTGA", "aa": "MIFNKKTKHLYIHIPFCNYICIFSDFKRILKTPQTKKFFKNFLENIKMHINNFKIKQFKTIYLGGGVPNCLNNQELEILLKTISPYVDDNCEYTIECNPELINQTQINLFKKYKINRISLNVQSMNNDVLKQMNFIHTNQDSKKAINLLHKNGLYNVSCDFLFCISILTLKDLDDVFNFILNHKINHVSFYSLEIKEQSLLKKYHITIDEEKEIEQMNYIINKFAGLNFHRYEVCN", "aa_hexdigest": "305bce1da4f757d0138eb449cb82d253", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001E3EC27E", "query_cov": 1.0, "subject_cov": 0.8489208633093526, "identity": 1.0, "score": 482.0, "evalue": 1.22e-171, "valid": true, "product": "radical SAM protein", "uniref50_id": "UniRef50_UPI001E3EC27E" }, "pscc": { "uniref50_id": "UniRef50_UPI001E3EC27E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E3EC27E" ], "product": "radical SAM protein" }, "genes": [], "id": "NKIECMJPJD_1053", "locus": "GCA000828735_01049" }, { "type": "cds", "sequence": "contig_1", "start": 617237, "stop": 617404, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATGGGTTTAAGGTTAAAAGATGGATTAAATCTAAATAAAAAAATTAATTATGAAGCTTATTCATATTTTAAAAATCAATTGAAACACGTTAGTATTAATAAAAAAAATCATCTTCTTATTGATGATTTAAATTTACTAAATTTAACTATTTTAGATATATTTTAA", "aa": "MMGLRLKDGLNLNKKINYEAYSYFKNQLKHVSINKKNHLLIDDLNLLNLTILDIF", "aa_hexdigest": "0a4d9074a8adfcbcf2c2b3e9074ab8de", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6505.607699999999, "isoelectric_point": 9.398539543151855 }, "id": "NKIECMJPJD_1054", "locus": "GCA000828735_01050" }, { "type": "cds", "sequence": "contig_1", "start": 617734, "stop": 618003, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTTTAAAAGTTTTTTTAAACAAAAACCGAGATATTATTTCTAAAATTCCATTAAATCAAGTTAAAAAAATAGCTAATGACATTTTAGAAAAAGAAATTATTTTAGAACAGCCTTTAGTTGATTATTATTACCAAAGTTCATCTTGTTTTATTTTAATACCTTATTTGATTCAAATTCTTTATCAATCTTATCACCTTAATAAACCAATTTATAAAATAATGTGTAAATTTATTATTAGAAATAATTTAGCATTATTTAAAGAATGA", "aa": "MILKVFLNKNRDIISKIPLNQVKKIANDILEKEIILEQPLVDYYYQSSSCFILIPYLIQILYQSYHLNKPIYKIMCKFIIRNNLALFKE", "aa_hexdigest": "37a43775bc428f98a35f719be37ba477", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10681.725699999988, "isoelectric_point": 9.275147056579591 }, "id": "NKIECMJPJD_1055", "locus": "GCA000828735_01051" }, { "type": "cds", "sequence": "contig_1", "start": 618167, "stop": 618625, "strand": "-", "frame": 2, "gene": "hinT", "product": "HIT family protein", "db_xrefs": [ "COG:COG0537", "COG:FR", "RefSeq:WP_006688907.1", "SO:0001217", "UniParc:UPI00000C1BF7", "UniRef:UniRef100_A0AAC9T389", "UniRef:UniRef50_A0A084F0Z8", "UniRef:UniRef90_A0AAC9T389" ], "nt": "ATGCAAGATTGCATTTTTTGTAAAATTTTAAAAGGTGAAATTATTTCAAAAATAATAGATGAAAACGAATTTGCTATAGCAATTTTAGACATTCAACCTGCAAGTGATGGTCATATGTTGATTATTCCTAAAAAACATTATCGCAATTTTTCACTAACTGATCCTATTTATCTAGATGGTATGATGCGTTTAGCAAAAAATATGACATTTGTTTTAGAAGAAGTTTTTCCTAATGTTTTAGGTTTTAATTATTTAATGAATTCAAATTCTAGTGCTGGTCAAGTAATCATGCACACACATTTACACATCATTCCAAAACAAAGTAATGGACGTGGTTTTGTTTTTAAAGCAATCAAAGAAGAAGGCGATATTAGTAATATTGATGAAATTTATAAAAAAATCATTATTAAAACACAAAAATTAAAAAAATCACGTTTAAGTAAGCATTATATGGTTTAA", "aa": "MQDCIFCKILKGEIISKIIDENEFAIAILDIQPASDGHMLIIPKKHYRNFSLTDPIYLDGMMRLAKNMTFVLEEVFPNVLGFNYLMNSNSSAGQVIMHTHLHIIPKQSNGRGFVFKAIKEEGDISNIDEIYKKIIIKTQKLKKSRLSKHYMV", "aa_hexdigest": "2ba2918b8647ee272448115095505151", "start_type": "ATG", "rbs_motif": "GGAGG", "ups": { "uniparc_id": "UPI00000C1BF7", "ncbi_nrp_id": "WP_006688907.1", "uniref100_id": "UniRef100_A0AAC9T389", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1BF7", "RefSeq:WP_006688907.1", "UniRef:UniRef100_A0AAC9T389" ] }, "ips": { "uniref100_id": "UniRef100_A0AAC9T389", "uniref90_id": "UniRef90_A0AAC9T389" }, "psc": { "uniref90_id": "UniRef90_A0AAC9T389", "gene": "HinT", "product": "HIT family protein", "uniref50_id": "UniRef50_A0A084F0Z8", "cog_id": "COG0537", "cog_category": "FR" }, "pscc": { "uniref50_id": "UniRef50_A0A084F0Z8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A084F0Z8" ], "product": "Histidine triad protein" }, "genes": [ "hinT" ], "id": "NKIECMJPJD_1056", "locus": "GCA000828735_01052" }, { "type": "cds", "sequence": "contig_1", "start": 618603, "stop": 619208, "strand": "-", "frame": 1, "gene": null, "product": "ABC transporter permease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T3I0", "UniRef:UniRef90_A0AAC9T3I0" ], "nt": "TTGTTTAATATTATTAAAAATAATAATCAATTATTTGAAGTTTTTTTAAATAATTATGACTACATAAAAAATCTTGAGGACAATCAAAAAATTATTGATATTATTCAACAAAATCCTGATTTAATTGATGATTTTAAAAAGATTGATTTTGATCTTTTAACACAAAAAAGGAATTTTGTTCGTGCACTAATTAAAATAATTGATTATAAACAACCCAAAGAAATTCAATTCCTTAACAAAAAAGTTTTTTTAGATTTCTTTAAAAATAAATCTGAAATTACCAATGCTTTAAACATCACATCTTTAATTGGATTTGTATTGTTTATAGTAATTTATACAATTTTTATTTTTGCATCTTTTATTTCTCATATTCTAATGTTAAAACAAATTAAAGATTTTAATAAAATTTTTAAAGAAGCTAAATTAAAAAAAATATATCTTATAAGCACTTTATTATTACAATTTTTATTTTTAATTTTATTCATTATTAATTTTATAGTATTAATTATTCTAGTTGTAGATTATAATAAATTGAAAGCAATAGTTTTAAATAGTGGTATAAAAAGAAAAGTGGAGGATAGTGATGCAAGATTGCATTTTTTGTAA", "aa": "MFNIIKNNNQLFEVFLNNYDYIKNLEDNQKIIDIIQQNPDLIDDFKKIDFDLLTQKRNFVRALIKIIDYKQPKEIQFLNKKVFLDFFKNKSEITNALNITSLIGFVLFIVIYTIFIFASFISHILMLKQIKDFNKIFKEAKLKKIYLISTLLLQFLFLILFIINFIVLIILVVDYNKLKAIVLNSGIKRKVEDSDARLHFL", "aa_hexdigest": "dcd7b56f2292afc571a600c5bf0c1e00", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 618603, "stop": 619427, "observations": { "mutation": [ 619271 ], "stop": [ 619271 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAC9T3I0", "query_cov": 1.0, "subject_cov": 0.43982494529540483, "identity": 0.905, "score": 276.0, "evalue": 2e-88, "gene_start": 1, "gene_end": 201, "reference_start": 257, "reference_end": 457, "reference_sequence": "MFMFDQIFKKRKRHLNFQIFIFFFVLILITFLILWIISGNVQTIIDFIKKKIYDASPQLKYLTNIQIKPFKNLEEIFDFIIKNKNLIKDLQNEPEILDVIKNNPQILKTLNDNREFLKQIVEYKPFAEILSKQPEILKFVQNNKELIKQIQNLPELLDLIKKHQNLIDHLKTNEKYIEVIKANPQMLKDLYHVDDNLFNIIKTSLENDKENNILMLAIKYSVLINNFTKNLTLQEWKQIIEFYKTVDLNKFIVDDNLFNIIKNNNQLFEVFLNNYDYIKNLEDNQKIIDIIQQNPDLIDDFKKIDFDLLTQKRNFVRALIKIIDYKQPKEIQFLNKKVFLDFFKNKSEITNALNITSLIGFVLFIVIYTIFIFASFISHILMLKQIKDFNKIFKEAKLKKIYLISTLLLQFLFLILFIINFIVLIILVVDYNKLKAIVLNSGIKRKVEDSDARLHFL" }, "paralog": false, "identity": 0.9963503649635036, "score": 428.0, "evalue": 1.37e-150, "description": "Internal stop codon at 619271. Nonsense mutation around 619271.", "psc": { "uniref90_id": "UniRef90_A0AAC9T3I0", "product": "ABC transporter permease", "uniref50_id": "UniRef50_A0AAC9T3I0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAC9T3I0", "UniRef:UniRef50_A0AAC9T3I0" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAC9T3I0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T3I0" ], "product": "ABC transporter permease" } }, "id": "NKIECMJPJD_1057", "locus": "GCA000828735_01053" }, { "type": "cds", "sequence": "contig_1", "start": 619269, "stop": 619721, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTAAAGATTTACAAAACGAACCTGAAATTTTAGATGTTATTAAAAACAATCCACAGATCTTAAAAACACTTAATGATAACCGCGAATTTTTAAAACAAATTGTTGAATATAAGCCATTTGCTGAAATTCTTAGCAAGCAACCTGAAATTTTAAAATTTGTTCAAAATAATAAAGAATTAATTAAACAGATACAAAATTTACCTGAATTATTAGATTTAATTAAAAAACACCAAAATTTAATTGATCACTTAAAAACTAACGAAAAATATATAGAAGTTATTAAAGCTAATCCTCAAATGTTAAAAGATCTCTATCATGTAGATGATAATTTATTTAATATTATAAAGACAAGCTTAGAAAATGATAAAGAAAATAATATTTTAATGTTGGCTATTAAATATTCAGTATTAATTAATAATTTTACTAAGAATTTAACTTTACAAGAATGA", "aa": "MIKDLQNEPEILDVIKNNPQILKTLNDNREFLKQIVEYKPFAEILSKQPEILKFVQNNKELIKQIQNLPELLDLIKKHQNLIDHLKTNEKYIEVIKANPQMLKDLYHVDDNLFNIIKTSLENDKENNILMLAIKYSVLINNFTKNLTLQE", "aa_hexdigest": "38badd23233fa10c15d62275f59d5b53", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17763.476300000002, "isoelectric_point": 5.961921501159668 }, "id": "NKIECMJPJD_1058", "locus": "GCA000828735_01054" }, { "type": "cds", "sequence": "contig_1", "start": 620024, "stop": 620194, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGCTATAGTATTCAAGGAACACTTTTTTACAATACTGCTTTTATTCCTTCTATGATTTATAATTCTTTGGTAACTTTTGGTTCAATGGCAATTAATTTTGTATTATATATAATTTCAATTCCCGCTATTGTTGCCTTAAAAAACAAATATTTATGTAAATTAGTTTAA", "aa": "MGYSIQGTLFYNTAFIPSMIYNSLVTFGSMAINFVLYIISIPAIVALKNKYLCKLV", "aa_hexdigest": "acda50cfe7ed6e0e849d23752029badc", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6257.488399999998, "isoelectric_point": 9.302675056457517 }, "id": "NKIECMJPJD_1059", "locus": "GCA000828735_01055" }, { "type": "cds", "sequence": "contig_1", "start": 620195, "stop": 620467, "strand": "-", "frame": 2, "gene": null, "product": "ECF transporter S component", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T0E1", "UniRef:UniRef90_A0AAC9T0E1" ], "nt": "ATGGGAATTTTTGTTTTAGCAATCGTTTGTATTCCCAATTTTTATTATAAATTAATGTATTATATTATTGCGCCGTTAGTGATGTTAGCAATTGGTTTTAGTGCTGAGCCCTTCTTTGGATATTTAATGCCACATTATGGTTTTGGTTTAATTTTATTTACGGATGTCATTTTAATTATTGTTAAAAAGTCATGTCAGTCAATTAAAAAGCAAATATTTTTATCCTATTTATTAATTTTTAGCTTTTCAATCATTGGTTATTTTATTGTTTGA", "aa": "MGIFVLAIVCIPNFYYKLMYYIIAPLVMLAIGFSAEPFFGYLMPHYGFGLILFTDVILIIVKKSCQSIKKQIFLSYLLIFSFSIIGYFIV", "aa_hexdigest": "cf9c511021059bb1fe1b46dafbe7286d", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 620024, "stop": 620695, "observations": { "mutation": [ 620197, 620479 ], "stop": [ 620197, 620479 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAC9T0E1", "query_cov": 1.0, "subject_cov": 0.40358744394618834, "identity": 1.0, "score": 168.0, "evalue": 3.81e-51, "gene_start": 1, "gene_end": 90, "reference_start": 77, "reference_end": 166, "reference_sequence": "MINKNKKFHFQYFKKDNDQSISTKETLKRYFNFRSQSVKLIIFNLVLTAFFLALFLIGRFLTKNLDFLNGFSWQLQMGIFVLAIVCIPNFYYKLMYYIIAPLVMLAIGFSAEPFFGYLMPHYGFGLILFTDVILIIVKKSCQSIKKQIFLSYLLIFSFSIIGYFIVWMGYSIQGTLFYNTAFIPSMIYNSLVTFGSMAINFVLYIISIPAIVALKNKYLCKLV" }, "paralog": false, "identity": 0.9910313901345291, "score": 434.0, "evalue": 2.06e-158, "description": "Internal stop codon at 620197, 620479. Nonsense mutation around 620197, 620479.", "psc": { "uniref90_id": "UniRef90_A0AAC9T0E1", "product": "ECF transporter S component", "uniref50_id": "UniRef50_A0AAC9T0E1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAC9T0E1", "UniRef:UniRef50_A0AAC9T0E1" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAC9T0E1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T0E1" ], "product": "ECF transporter S component" } }, "id": "NKIECMJPJD_1060", "locus": "GCA000828735_01056" }, { "type": "cds", "sequence": "contig_1", "start": 620477, "stop": 620695, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATCAATAAAAACAAAAAATTTCATTTTCAGTATTTTAAAAAAGATAACGATCAATCAATTTCTACTAAAGAAACATTAAAAAGATATTTTAATTTTAGGTCTCAAAGTGTAAAATTGATTATTTTTAATTTAGTTTTAACAGCATTTTTTCTTGCATTATTTTTAATTGGTCGTTTTTTAACTAAAAATTTAGATTTTTTAAATGGTTTTAGTTGA", "aa": "MINKNKKFHFQYFKKDNDQSISTKETLKRYFNFRSQSVKLIIFNLVLTAFFLALFLIGRFLTKNLDFLNGFS", "aa_hexdigest": "4ca776154467a1a7dd255c7a6b5ce919", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8650.058899999989, "isoelectric_point": 10.234565544128415 }, "id": "NKIECMJPJD_1061", "locus": "GCA000828735_01057" }, { "type": "tRNA", "sequence": "contig_1", "start": 620827, "stop": 620905, "strand": "-", "gene": "trnH", "product": "tRNA-His(gtg)", "amino_acid": "His", "anti_codon": "gtg", "score": 67.2, "nt": "GCGGTTATGGTGAAGTTGGTTAACACGCCTGATTGTGGCTCAGGTATTTTCGCGGGTTCGAGTCCCGTTAATCGCCCCA", "db_xrefs": [ "SO:0000262" ], "anti_codon_pos": [ 620894, 620896 ], "id": "NKIECMJPJD_23", "locus": "GCA000828735_01058" }, { "type": "cds", "sequence": "contig_1", "start": 620943, "stop": 621137, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATACTATTTATGATGTATTGCTAAAGGATTTAACAAAGTATGAAATTTTATTTTTAACTTATAATTTATACACACTTGTTAACAATGTTGAATTATTAAAAAATATTCCTACCGAATATGAAAAAAAATTTGTTATGCAGGGCGAGCGTATTAAAAAAGTCAATTTTCGTTTTCTTAAAAATAATCAATAA", "aa": "MYTIYDVLLKDLTKYEILFLTYNLYTLVNNVELLKNIPTEYEKKFVMQGERIKKVNFRFLKNNQ", "aa_hexdigest": "4f31d40bca77bdad11431a9c7f8e3d4b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7856.160299999999, "isoelectric_point": 9.222540855407715 }, "id": "NKIECMJPJD_1062", "locus": "GCA000828735_01059" }, { "type": "cds", "sequence": "contig_1", "start": 621255, "stop": 621617, "strand": "-", "frame": 1, "gene": "trmB", "product": "tRNA (guanosine(46)-N7)-methyltransferase TrmB", "db_xrefs": [ "COG:COG0220", "COG:J", "EC:2.1.1.33", "GO:0006400", "GO:0008176", "GO:0043527", "SO:0001217", "UniRef:UniRef50_Q9PQM2", "UniRef:UniRef90_Q9PQM2" ], "nt": "ATGCGTTTACGAAATAATGCCAATGCTCCACTTTATTTAAAATCACAACACGAATATATAATTAATGATCCACACCTTTTAAAAGATAATTTAGGAAAAATTTTTAAAAATCCTGAATTGCCTCTTTATATTGAAATTGGAATGGGTAAGGGTGATTTTATTATTGAAAATGCCTTACGAAACCAACAAATTAATTATTTAGGTATTGAAAAATTTCCAACAGTAATTGTTAAAGCTCACAAAAAAGCTTTAAAACATAAATTAGATAATTTAGCAATGATTTGTTTTGACGCAAATAAAATTTTAGAATTATTAAATTCTGAAAGTGTAGATAAAATTTATTTAAATTTTTCTGACCCCTGA", "aa": "MRLRNNANAPLYLKSQHEYIINDPHLLKDNLGKIFKNPELPLYIEIGMGKGDFIIENALRNQQINYLGIEKFPTVIVKAHKKALKHKLDNLAMICFDANKILELLNSESVDKIYLNFSDP", "aa_hexdigest": "45c5d220d6fba143948f177ddc8cadd4", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "trmB" ], "truncated": "5-prime", "pseudogene": { "start": 620952, "stop": 621617, "observations": { "mutation": [ 621257 ], "stop": [ 621257 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQM2", "query_cov": 1.0, "subject_cov": 0.5357142857142857, "identity": 1.0, "score": 245.0, "evalue": 7.79e-81, "gene_start": 1, "gene_end": 120, "reference_start": 1, "reference_end": 120, "reference_sequence": "MRLRNNANAPLYLKSQHEYIINDPHLLKDNLGKIFKNPELPLYIEIGMGKGDFIIENALRNQQINYLGIEKFPTVIVKAHKKALKHKLDNLAMICFDANKILELLNSESVDKIYLNFSDPWPKKRHAKKRLTHPCFLEKFAVILKQNALVEFKTDNENLFMYTIYDVLLKDLTKYEILFLTYNLYTLVNNVELLKNIPTEYEKKFVMQGERIKKVNFRFLKNNQ" }, "paralog": false, "identity": 0.995475113122172, "score": 442.0, "evalue": 3.07e-161, "description": "Internal stop codon at 621257. Nonsense mutation around 621257.", "psc": { "uniref90_id": "UniRef90_Q9PQM2", "gene": "trmB", "product": "tRNA (guanosine(46)-N7)-methyltransferase TrmB", "ec_ids": [ "2.1.1.33" ], "uniref50_id": "UniRef50_Q9PQM2", "cog_id": "COG0220", "cog_category": "J", "go_ids": [ "GO:0006400", "GO:0008176", "GO:0043527" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQM2", "EC:2.1.1.33", "UniRef:UniRef50_Q9PQM2", "COG:COG0220", "COG:J", "GO:0006400", "GO:0008176", "GO:0043527" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQM2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQM2" ], "product": "tRNA (guanine-N(7)-)-methyltransferase" } }, "id": "NKIECMJPJD_1063", "locus": "GCA000828735_01060" }, { "type": "cds", "sequence": "contig_1", "start": 621623, "stop": 622141, "strand": "-", "frame": 2, "gene": "trmL", "product": "Putative tRNA (cytidine(34)-2'-O)-methyltransferase", "db_xrefs": [ "COG:COG0219", "COG:J", "EC:2.1.1.207", "KEGG:K03216", "RefSeq:WP_006688833.1", "SO:0001217", "UniParc:UPI00000C1BF4", "UniRef:UniRef100_Q9PQM3", "UniRef:UniRef50_Q9PQM3", "UniRef:UniRef90_Q9PQM3" ], "nt": "ATGTTAAATATTGTTTTATTTGAACCAGAAATTATTTTAAACACTGGTAATATCGCTCGTATGTGTGTAGGTTTTGATGCAAATTTACATCTTGTTAGACCATATGGATTTATTTTAGATAAAAAACGTTTTCACAAAGATTTTATACGTGCCTCAACCAATCATTTAAATGCCTTAAAATTATATGAATATGATGATTTTAATGAATTTATTAATAAAAACAACATTAGTGGTGATGAAATATATTTTTTTACTAGATATGGTACTAAAACTCCGTGCGATTATCAATATCAAAATATAATTCAACATAATGTATATTTAGTTTTTGGTAAAGAATCAACAGGGATAGATCATAATATTTTAAAAAAATATCCTCATAATTGGATCCGTATTCCTACAAGCATTAATTTGAGAAGTTTAAATATTGCTAATAGTGTTGCTATGGGAATCTATGAAGTAGTGCGGCAAAATAATTTTAAAGATTTATTCAAATATGAACCTCATAAGAAATTTGAGTAA", "aa": "MLNIVLFEPEIILNTGNIARMCVGFDANLHLVRPYGFILDKKRFHKDFIRASTNHLNALKLYEYDDFNEFINKNNISGDEIYFFTRYGTKTPCDYQYQNIIQHNVYLVFGKESTGIDHNILKKYPHNWIRIPTSINLRSLNIANSVAMGIYEVVRQNNFKDLFKYEPHKKFE", "aa_hexdigest": "6daa8fb35ba1cc8e23aa0d1092411841", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI00000C1BF4", "ncbi_nrp_id": "WP_006688833.1", "uniref100_id": "UniRef100_Q9PQM3", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1BF4", "RefSeq:WP_006688833.1", "UniRef:UniRef100_Q9PQM3" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQM3", "uniref90_id": "UniRef90_Q9PQM3" }, "psc": { "uniref90_id": "UniRef90_Q9PQM3", "gene": "TrmL", "product": "Putative tRNA (cytidine(34)-2'-O)-methyltransferase", "ec_ids": [ "2.1.1.207" ], "uniref50_id": "UniRef50_Q9PQM3", "cog_id": "COG0219", "cog_category": "J", "kegg_orthology_id": "K03216" }, "pscc": { "uniref50_id": "UniRef50_Q9PQM3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQM3" ], "product": "Putative tRNA (cytidine(34)-2'-O)-methyltransferase" }, "genes": [ "trmL" ], "id": "NKIECMJPJD_1064", "locus": "GCA000828735_01061" }, { "type": "cds", "sequence": "contig_1", "start": 622146, "stop": 622478, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF10458.14" ], "nt": "TTGTTATTAATCTTAGTTAATGCTGAAATAAATGAAATTAAAAAAACAAGTTCTAATAATTATACTTATGAGCTAGTTATTGATGATTTTATTCTTAAAACTTCGTATGAAAAACCGATTGATTATGTTTTTCAAATGAAAAAAGCTAGTGAACAGTTAAACTATTTGGAAAACGAAATTCAACGAGCAACTAATTTATTAAATAATTCAGGTTTTATCAACAAAGCCCCTGCTCAATTAATAATTAAAGAAAAAAATAAATTAATTAATTTAAAAAAAGAACACGCAAATTTATTAAAAACTCTTACTGACTTAAAACAAAAGGTGAAATAA", "aa": "MLLILVNAEINEIKKTSSNNYTYELVIDDFILKTSYEKPIDYVFQMKKASEQLNYLENEIQRATNLLNNSGFINKAPAQLIIKEKNKLINLKKEHANLLKTLTDLKQKVK", "aa_hexdigest": "472c58c25469d68599b1eb645e7cf879", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF10458.14", "name": "Val_tRNA-synt_C", "length": 57, "aa_cov": 0.5181818181818182, "hmm_cov": 1.0, "evalue": 3.5710280848916746e-11, "score": 38.809513092041016, "start": 50, "stop": 106 } ], "seq_stats": { "molecular_weight": 12799.770099999976, "isoelectric_point": 9.222669792175292 }, "id": "NKIECMJPJD_1065", "locus": "GCA000828735_01062" }, { "type": "cds", "sequence": "contig_1", "start": 623058, "stop": 623291, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00133.28" ], "nt": "ATGATGAACTTTAGCCAATATTTGGTTGAAAAAAAACCTTTTAAGGATGTTTTAATTCATGGTTTAATTCGTGATTCACAGGGACGTAAGATGTCAAAATCATTAGGGAATGGTATTGATCCTTTTGACATTATTGATAAATATGGATTAGATGCTATGCGTTTATTTTTTGCATCATGTACAACAATTGGTGAAGATTTAAATTTTTCAACAGAACGATTAGGCGCTAATTGA", "aa": "MMNFSQYLVEKKPFKDVLIHGLIRDSQGRKMSKSLGNGIDPFDIIDKYGLDAMRLFFASCTTIGEDLNFSTERLGAN", "aa_hexdigest": "921e8c30a9758ee52e332fdb0d7f8928", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI001D01CCB6", "query_cov": 0.948051948051948, "subject_cov": 0.8390804597701149, "identity": 0.562, "score": 89.4, "evalue": 8.49e-22, "valid": false, "product": "class I tRNA ligase family protein", "uniref50_id": "UniRef50_A0A3B0PEY5" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00133.28", "name": "tRNA-synt_1", "length": 68, "aa_cov": 0.8831168831168831, "hmm_cov": 0.9852941176470589, "evalue": 1.1472114051804609e-24, "score": 82.42316436767578, "start": 3, "stop": 70 } ], "seq_stats": { "molecular_weight": 8706.936999999994, "isoelectric_point": 6.540030860900879 }, "id": "NKIECMJPJD_1066", "locus": "GCA000828735_01063" }, { "type": "cds", "sequence": "contig_1", "start": 623577, "stop": 623675, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATAGTTTAATAAAAGATACAATCAAAATGCAAGATGATTGTAATAATAAAGTAGATTTTTATCCAAATCGTTTTAATAAGACTTTACTTACATGA", "aa": "MDSLIKDTIKMQDDCNNKVDFYPNRFNKTLLT", "aa_hexdigest": "124a7c2cc837858b6b98ce7b7a55a9a6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3822.3457000000008, "isoelectric_point": 5.897920799255371 }, "id": "NKIECMJPJD_1067", "locus": "GCA000828735_01064" }, { "type": "cds", "sequence": "contig_1", "start": 623685, "stop": 624197, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13603.12" ], "nt": "GTGATTCATAAAGAAGTTGAACAAAAACTGTATTATATAAAATATTTAAGTCAAGATCAAAAGGATTTTGTTGTTGTAGCCACTTCACGCCCTGAAACTATGTTTGGTGATAAATATTTAGTTATAAATCCTAAAGATAAACGTTATTTTCATTTACATAATAAAATTTTTATTAATCCAATTAATAATATAGAAATGACTGTTATTTTGGATGATTATATTGATATTGAATTTGGAACAGGAGTGATGAAGTGTACGCCTGCTCACGATTTTAATGACTATGAATTAGCTAAAAAACATAATTTAGAAATTATTAATATTATGAACGCTGATGGTACTTTAAATGAAAAATGTGGGGAATTTAAGGGTTTAGATCGATTAGAAGCTCGTTCTTTAATAATTGATAAATTACAGAAAAATAATCATTTATTAAAAGTAGAATCTTATCGAACCAGCGTTGGTTTTAGTGAGAGAACTAATGAAATTGTTGAACCTTATTTATCACATCAATGA", "aa": "MIHKEVEQKLYYIKYLSQDQKDFVVVATSRPETMFGDKYLVINPKDKRYFHLHNKIFINPINNIEMTVILDDYIDIEFGTGVMKCTPAHDFNDYELAKKHNLEIINIMNADGTLNEKCGEFKGLDRLEARSLIIDKLQKNNHLLKVESYRTSVGFSERTNEIVEPYLSHQ", "aa_hexdigest": "70c3de9c8801e180e40ee0f35d9bde04", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13603.12", "name": "tRNA-synt_1_2", "length": 58, "aa_cov": 0.3411764705882353, "hmm_cov": 1.0, "evalue": 6.247207262286937e-16, "score": 53.96089172363281, "start": 55, "stop": 112 } ], "seq_stats": { "molecular_weight": 19944.593500000006, "isoelectric_point": 5.971356773376464 }, "id": "NKIECMJPJD_1068", "locus": "GCA000828735_01065" }, { "type": "cds", "sequence": "contig_1", "start": 624234, "stop": 624359, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00133.28" ], "nt": "TTGGGTTTAGATTATAGTAATTATTTATTTACATTAGATCCAATAGTTGTTCAAACAGTGCGCGAAGTTTTTGTAAAAATGTTTAATGAACATATTATTTATCGTGATAAAAAATTAGTTAATTGA", "aa": "MGLDYSNYLFTLDPIVVQTVREVFVKMFNEHIIYRDKKLVN", "aa_hexdigest": "d0df84e6b47b0b2d506ec1081f066941", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00133.28", "name": "tRNA-synt_1", "length": 39, "aa_cov": 0.9512195121951219, "hmm_cov": 1.0, "evalue": 5.187275219159522e-08, "score": 27.561874389648438, "start": 3, "stop": 41 } ], "seq_stats": { "molecular_weight": 4949.7439, "isoelectric_point": 6.521330833435059 }, "id": "NKIECMJPJD_1069", "locus": "GCA000828735_01066" }, { "type": "cds", "sequence": "contig_1", "start": 624408, "stop": 624644, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00133.28" ], "nt": "TTGCCCCCTCCTAATGTTACAGGTCATTTACATATTGGACATGCCTATGATTTTACCTTATCTGATATTTTAATGCGTTATAAGAAACTAAAAGGATATGATTCCTTTATTATTCCTGGGACAGACCATGCAGGGATCGCAACACAGACAAAATTTGAAAAAAATTTAAAAGTAAACGCACAAACCAATCGTTTCAATTTAGGGCGAGAACTGTTTTTAGAAAAGTTAAAAATTTGA", "aa": "MPPPNVTGHLHIGHAYDFTLSDILMRYKKLKGYDSFIIPGTDHAGIATQTKFEKNLKVNAQTNRFNLGRELFLEKLKI", "aa_hexdigest": "4bcffa11b0e9900daae018cc700a0615", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00133.28", "name": "tRNA-synt_1", "length": 76, "aa_cov": 0.9743589743589743, "hmm_cov": 1.0, "evalue": 9.245539196030584e-25, "score": 82.73184204101562, "start": 2, "stop": 77 } ], "seq_stats": { "molecular_weight": 8914.235799999999, "isoelectric_point": 9.572539710998534 }, "id": "NKIECMJPJD_1070", "locus": "GCA000828735_01067" }, { "type": "cds", "sequence": "contig_1", "start": 624751, "stop": 625377, "strand": "-", "frame": 3, "gene": "yihA", "product": "ribosome biogenesis GTP-binding protein YihA/YsxC", "db_xrefs": [ "COG:COG0218", "COG:D", "GO:0000917", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0005829", "GO:0042254", "GO:0043022", "GO:0046872", "RefSeq:WP_006688825.1", "SO:0001217", "UniParc:UPI0000129F33", "UniRef:UniRef100_Q9PQM5", "UniRef:UniRef50_Q9PQM5", "UniRef:UniRef90_Q9PQM5" ], "nt": "ATGGCAAAATTTATTAAATCTGCACACTATTTTGACCAATATCCAATTGATAAGCAGTTTGAAATATGTGTAATTGGTAGATCAAATGTCGGTAAGTCAAGTTTAATTAATGCATTAGCAAATAAAAAAATTGCAAGGACTTCAAATACGCCTGGAAGAACACAGTTAGTAAATTTTTTTGATTTCAATAATTTTAGATTGGTTGATTTACCGGGATATGGGTTTGCAAAAGTTAGTAAAGAAAAACAAACGGATTTAGCAGCCATTATTGATCAATATTTAGGGTATCGCCAAAACTTGTGTGCTGTTTTTCAAATTTGCGATATTAACGTATTAACAAATGATGATGTAGAAATGAGTCGATATTTTGAGAATCAAAAATACGCTCATTTTGTTGTTTTAAATAAATTAGATAAAGTTAATAAATCATATTTTAATAATAACAAGCATAAAATAGCTAAATTTTTAAATATTAGCACCGATCGTTTATTATGTGTTTCTGCACAAAATAATATAAATATTATCACATTATTTGCGTTAATGAAAAAAGTTGTAATCCAAGCAAAACAAGAAAAAATACTTTCAAAAAAAGAGGAGAAGATGAGTAGTGAAGAAGAAATTAAGTAA", "aa": "MAKFIKSAHYFDQYPIDKQFEICVIGRSNVGKSSLINALANKKIARTSNTPGRTQLVNFFDFNNFRLVDLPGYGFAKVSKEKQTDLAAIIDQYLGYRQNLCAVFQICDINVLTNDDVEMSRYFENQKYAHFVVLNKLDKVNKSYFNNNKHKIAKFLNISTDRLLCVSAQNNINIITLFALMKKVVIQAKQEKILSKKEEKMSSEEEIK", "aa_hexdigest": "8c0818ecb1b5504ffdff4646aa8646e5", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000129F33", "ncbi_nrp_id": "WP_006688825.1", "uniref100_id": "UniRef100_Q9PQM5", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000129F33", "RefSeq:WP_006688825.1", "UniRef:UniRef100_Q9PQM5" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQM5", "uniref90_id": "UniRef90_Q9PQM5" }, "psc": { "uniref90_id": "UniRef90_Q9PQM5", "gene": "yihA", "product": "ribosome biogenesis GTP-binding protein YihA/YsxC", "uniref50_id": "UniRef50_Q9PQM5", "cog_id": "COG0218", "cog_category": "D", "go_ids": [ "GO:0000917", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0005829", "GO:0042254", "GO:0043022", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQM5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQM5" ], "product": "Probable GTP-binding protein EngB" }, "genes": [ "yihA" ], "id": "NKIECMJPJD_1071", "locus": "GCA000828735_01068" }, { "type": "cds", "sequence": "contig_1", "start": 625382, "stop": 625711, "strand": "-", "frame": 2, "gene": null, "product": "UPF0154 protein UU265", "db_xrefs": [ "GO:0016020", "KEGG:K09976", "SO:0001217", "UniParc:UPI000169E512", "UniRef:UniRef100_A0AAC9X6T2", "UniRef:UniRef50_Q9PQM6", "UniRef:UniRef90_Q9PQM6" ], "nt": "ATGAATTTTTCAAGTTTTATATTTAAAGTTTCTGATGTTTTTAAATCAGTTATTCATGAAGCTTCAGATGTTGTTACTAAAGCAGATTTAGATAATGCTAATGCACATGCACATTCATTAGCAGTTGGCTTAGGTATTGGAATTGTTTTATTTTTAATCGCAGGACTTATTATCGGTTATTTTATTTCGATGAAAATTATGAAACGCCAATTGAAGAAGAATCCACCAATTTCAAAGGATACAATTCGTATGATTTATCAACAAGTAGGACGTAAGCCAAGTGAGTCACAAATTAATGAGATTTATAATCGGGCAGTAAAACAAAAATAA", "aa": "MNFSSFIFKVSDVFKSVIHEASDVVTKADLDNANAHAHSLAVGLGIGIVLFLIAGLIIGYFISMKIMKRQLKKNPPISKDTIRMIYQQVGRKPSESQINEIYNRAVKQK", "aa_hexdigest": "a28c817092bcf7612b040f8e4716f64b", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI000169E512", "uniref100_id": "UniRef100_A0AAC9X6T2", "db_xrefs": [ "SO:0001217", "UniParc:UPI000169E512", "UniRef:UniRef100_A0AAC9X6T2" ] }, "ips": { "uniref100_id": "UniRef100_A0AAC9X6T2", "uniref90_id": "UniRef90_Q9PQM6" }, "psc": { "uniref90_id": "UniRef90_Q9PQM6", "product": "UPF0154 protein UU265", "uniref50_id": "UniRef50_Q9PQM6", "kegg_orthology_id": "K09976", "go_ids": [ "GO:0016020" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQM6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQM6" ], "product": "UPF0154 protein UU265" }, "genes": [], "id": "NKIECMJPJD_1072", "locus": "GCA000828735_01069" }, { "type": "cds", "sequence": "contig_1", "start": 625862, "stop": 626716, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00690.31" ], "nt": "GTGAAAAAAAATAATGAAAATCAAACCGACTCCTTTATGTCATTTGATCCAGAAAATATAAATCCATCAACAGGTCTAAATGATGAGCAGGTTTTAAAAAGTCGTCAAATTTATGGTTTTAATGAAATCAAAAAGAAAAAAAAATCCCACATTATTACAAAATTTTTAAAGCAGTTTTTAGATTTTATGGTTATTTTACTTGTAATCGCTGCTGCTGTTACTTTAGCTTTAGTGATTATTAAACCTCCTCATGATACAGCTGAATTAGTTGTTCAATATGTAGAATTTGGAATAATATGTTTTATTTTATTGTTAAATGCAATTTTTGGTACCATTCAAGAAGTGAAAGCTGAAAAAAATACGGATGCATTGTCTAAATTAGCGTCTCATCAGGCCAAAGTATTACGTAATAATCAAATTAGAATTATTAATTCTAACCAAGTTGTAATGGGTGATGTTTTGATTTTAGAAGCTGGTGATCAAGTCCCAGCTGATGCTTTATTAGTGAACAGCTCATCACTTGAAGTAGATGAAGCTATTTTAACAGGAGAATCTTTACCAGTTGTAAAAAATGCCAAGGCAATTGTTAAAAAAAATACAGTAATTGGCGACTGTTTAAATCAAATTTTTTCTGGAACATATATTACTAATGGAACAGCAAAAGCAATTGTTACTAATATCGGTATGAATACTGAAATTGGTAAAATAGCCAAATTAATTAATGATCAAAAAACTAAATTAACGCCTTTACAAAAAAAAATTAATAAGTTATCTAAAATTATAGGTCTATTTGCTTCTATTTTATGTATTGCAGTATTTATTATTTATATTTATTTAATTGGAGATGGAAATTGA", "aa": "MKKNNENQTDSFMSFDPENINPSTGLNDEQVLKSRQIYGFNEIKKKKKSHIITKFLKQFLDFMVILLVIAAAVTLALVIIKPPHDTAELVVQYVEFGIICFILLLNAIFGTIQEVKAEKNTDALSKLASHQAKVLRNNQIRIINSNQVVMGDVLILEAGDQVPADALLVNSSSLEVDEAILTGESLPVVKNAKAIVKKNTVIGDCLNQIFSGTYITNGTAKAIVTNIGMNTEIGKIAKLINDQKTKLTPLQKKINKLSKIIGLFASILCIAVFIIYIYLIGDGN", "aa_hexdigest": "33e3e1ba80b40f583268e9c3886f08c9", "start_type": "GTG", "rbs_motif": "GGAGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00690.31", "name": "Cation_ATPase_N", "length": 53, "aa_cov": 0.18661971830985916, "hmm_cov": 1.0, "evalue": 1.1503745969713647e-17, "score": 58.99678421020508, "start": 21, "stop": 73 } ], "seq_stats": { "molecular_weight": 31213.307500000006, "isoelectric_point": 8.93623676300049 }, "id": "NKIECMJPJD_1073", "locus": "GCA000828735_01070" }, { "type": "cds", "sequence": "contig_1", "start": 626744, "stop": 627016, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTATTTCATTATCTATAGCAGCTATTCCTGAAGGAATTGTGGCAATTGTAACAATTGTTTTATCATTCGGTATTAAAGAAATGGCAAAACAAAATGCTTTAATTAAACGTTTGCCAGCTGTTGAAACTCTAGGAAGTGCTAATGTTATTTGTTCTGATAAAACAGGAACCTTAACACAAAACAAAATGACTGTTACTAAAGTTTTCACTAATAAATTAAAAATAACAGATTTAATTGATAATGAGAGTGTTTTTAATTTAATTAAATGA", "aa": "MAISLSIAAIPEGIVAIVTIVLSFGIKEMAKQNALIKRLPAVETLGSANVICSDKTGTLTQNKMTVTKVFTNKLKITDLIDNESVFNLIK", "aa_hexdigest": "f15972ef9e26e141da7eeba10e173d16", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9661.411499999993, "isoelectric_point": 9.25155162811279 }, "id": "NKIECMJPJD_1074", "locus": "GCA000828735_01071" }, { "type": "cds", "sequence": "contig_1", "start": 627200, "stop": 627703, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00702.32" ], "nt": "ATGAGTGTTGTTGTAAAACATCAAAGTGATTATTATTTAATTACTAAAGGAGCAATTGATGCTATTGACAAAATTGTTCAAAATCCAATTACAAAAGATGTTTATGAAGCAAATGATTTTTTAAGTAAACAAGCTTTAAGAATTTTATGTGTTGCAATTAAAAAACTATCATCTATACCAACTAATTTTAACCAAGATGAACTTGAAAAATGTTTAGAATTTGTTGGTCTAATCGGTATGATAGATCCGCCTCGTCCTGAAACACAAGAGGCAGTAAAAATTGCTATTAAAGCTGGAATCCGTCCGATAATGATTACTGGCGATCATATTAATACAGCATCAGCCATTGCTAAACAAGTTGGTATTTTAAACGAAGGACAAGAAGTTTTGAATGGTAATGAATTGAATATGATGAGTGATAATGAATTAATAAATAATATTGATCGTTATAGTGTTTATGCCCGAGTTTCTCCAACAGATAAAATCCGTATTGTTAAAGCTTGA", "aa": "MSVVVKHQSDYYLITKGAIDAIDKIVQNPITKDVYEANDFLSKQALRILCVAIKKLSSIPTNFNQDELEKCLEFVGLIGMIDPPRPETQEAVKIAIKAGIRPIMITGDHINTASAIAKQVGILNEGQEVLNGNELNMMSDNELINNIDRYSVYARVSPTDKIRIVKA", "aa_hexdigest": "1881f377a222ba155a375f1bde1a1ff1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00702.32", "name": "Hydrolase", "length": 60, "aa_cov": 0.3473053892215569, "hmm_cov": 0.9666666666666667, "evalue": 1.4909270027950977e-08, "score": 30.824228286743164, "start": 68, "stop": 125 } ], "seq_stats": { "molecular_weight": 18526.2521, "isoelectric_point": 5.714842414855957 }, "id": "NKIECMJPJD_1075", "locus": "GCA000828735_01072" }, { "type": "cds", "sequence": "contig_1", "start": 627655, "stop": 628152, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTAAAAATAGTAGCTCACATTCTTTTATTAACAATCGGTGCTGATTTTTTTAATGGTTTGTGTCGCATTAAATTAACATCCGCTAAATGTAACCCTAATGCTATTCCAGGTAAAGTTTCAGCAATTACATTAATTCATAATATTTGTAGTGATGACATAGGATTAATTCCTAGAATAATAATTCCAAATATTAATGAAACTAATCCAGATAAATTAGTGATCAATAATAAAACAATAATACGTTTAATGTTGTCCATAATAGAACGTCCTAAACTAACAGCATTAATAATTGTTGCGAAATTATCATCAATTAATATCATATCACTTGATGCTTTTGAAACATCAGTTCCAGTTATACCCATTGCGCATCCAATGTCAGCAGCTTTTAGTGCAGGTGCATCATTGACACCATCTCCAGTCATACTTACTACTTTATCATGGTGCTGTCAAGCTTTAACAATACGGATTTTATCTGTTGGAGAAACTCGGGCATAA", "aa": "MVKIVAHILLLTIGADFFNGLCRIKLTSAKCNPNAIPGKVSAITLIHNICSDDIGLIPRIIIPNINETNPDKLVINNKTIIRLMLSIIERPKLTALIIVAKLSSINIISLDAFETSVPVIPIAHPMSAAFSAGASLTPSPVILTTLSWCCQALTIRILSVGETRA", "aa_hexdigest": "7029021213459e9786f2ca20607c997c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17669.997899999988, "isoelectric_point": 9.167098045349121 }, "id": "NKIECMJPJD_1076", "locus": "GCA000828735_01073" }, { "type": "cds", "sequence": "contig_1", "start": 629057, "stop": 629428, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAATAAATTTTAAAAGAACTTTTTTATTATCATTTATAGGAGTTATAGTTACACCAATTCCTATAATTTTGAGTTGCTCATTAACAAAAATTAAAAAAGAAGACAATAAAAATTTCTTTAATCTTTATTTGAAAATTAAAGAAGAAGCTAGTTCTAAAGTTGATTTTGTTCTTAAAAATAATCATAAATACAATTATCTTCTTAATAAAGCAGATATAATCAATAATTATATTAATTACTTGGTGAATTTAAATTTAAATGAAAATAGTGAGAATGAATTATTAGCATTTACTTATTATGAATTATATAAATTAATAAAAGATTTTAATTATCTTTTAAAAGAAAAAACACAAGAATATGAATCATGA", "aa": "MKINFKRTFLLSFIGVIVTPIPIILSCSLTKIKKEDNKNFFNLYLKIKEEASSKVDFVLKNNHKYNYLLNKADIINNYINYLVNLNLNENSENELLAFTYYELYKLIKDFNYLLKEKTQEYES", "aa_hexdigest": "19ef68992141b2daa8e1dbdf71784603", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14694.880399999982, "isoelectric_point": 8.821740913391114 }, "id": "NKIECMJPJD_1077", "locus": "GCA000828735_01074" }, { "type": "cds", "sequence": "contig_1", "start": 629618, "stop": 629953, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTGAATCATACGATGGTCTTTATAAAAAAACAATTAATGATGAAGCTAATAAAATCAAAAATATTAAATCTGATGTTAAAAATTTAAAACATAATTATTTTGAAAAAATTGATAATGGTGAAGAATATTATAAGTTATACAATAGAGAAATTATGGAATTATTTCATGAAGCTAATCAACTTCATGAAAATATTTCTTCAGTACTACAGAGTATATTAAACAGAAAATTAATTGAAAATAAAATCGATAATATTAAAAGAGAAATCCAAGAACATAAAAAAGTTTCACAATATATTAAACAAAATGGCCACAAAAATAATCAAGATTTTTGA", "aa": "MIESYDGLYKKTINDEANKIKNIKSDVKNLKHNYFEKIDNGEEYYKLYNREIMELFHEANQLHENISSVLQSILNRKLIENKIDNIKREIQEHKKVSQYIKQNGHKNNQDF", "aa_hexdigest": "1cebf02797ce84eac79ca53153dc5d6b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13399.938099999985, "isoelectric_point": 7.893525123596191 }, "id": "NKIECMJPJD_1078", "locus": "GCA000828735_01075" }, { "type": "cds", "sequence": "contig_1", "start": 630710, "stop": 630841, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTATATTAACAAAACAAGTTCCTAATCATTTAAAAAATGAATATGATACAGCAAGATTGTTTTTTAGAATAGAGAGAACAATTGGTGAAATATGTAAAAAATACGCGTTAACTAATCCTTTAGAGTAA", "aa": "MFILTKQVPNHLKNEYDTARLFFRIERTIGEICKKYALTNPLE", "aa_hexdigest": "47048d3c8b54112e7098339f0ede6833", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5155.990499999999, "isoelectric_point": 8.978334617614745 }, "id": "NKIECMJPJD_1079", "locus": "GCA000828735_01076" }, { "type": "cds", "sequence": "contig_1", "start": 630849, "stop": 631436, "strand": "-", "frame": 1, "gene": "yIH1", "product": "Impact N-terminal domain-containing protein", "db_xrefs": [ "COG:COG1739", "COG:R", "RefSeq:WP_006688960.1", "SO:0001217", "UniParc:UPI000169E514", "UniRef:UniRef100_A0AAC9X6V1", "UniRef:UniRef50_A0A2C9DY85", "UniRef:UniRef90_A0A2C9DY85" ], "nt": "ATGTCATTTAAAACAATTGCATATACCCAAAAAAATATAAAAATTATTAAAAAATCAAAATTTATAACGATCGTTGAACCTATTAGCGATAAAAATGAAGTTCAATCATTATTTAAAAAATATAAAGAACAATATTACGATGCAACGCACATTTGTTTTGCTTATGTGATAGGTCGTGAATTTAATTATATGAAAGCATATGATGATGGTGAACCAAATGGAACAGCTGGAATACCTATTTTAAATATCATTAAGATCAAATCATTAACCAATGTGATTATATTTGTTGTACGTTATTTTGGTGGCATTAAATTAGGCACTGGAGGATTAATTCGTGCTTATTCACAATGTGCTAAAGAAATTATAGATAACTGTGAAATAATAATTTTACAAAAATATTTTTACTATAAAGTTGTTTATAAGCTAAAAAATACTAAATTTGCAAAGCAAATTTTAGCATCAATTAGTTATGAAAAACTAATAGAGAGTTATGAAGATGATAGAGTTATTTTAAAAATCCGAATTAACACCAAAATTAATATATTTATGTCTAAGATTGAATTTATTTTTATAAAACATGATTATTAA", "aa": "MSFKTIAYTQKNIKIIKKSKFITIVEPISDKNEVQSLFKKYKEQYYDATHICFAYVIGREFNYMKAYDDGEPNGTAGIPILNIIKIKSLTNVIIFVVRYFGGIKLGTGGLIRAYSQCAKEIIDNCEIIILQKYFYYKVVYKLKNTKFAKQILASISYEKLIESYEDDRVILKIRINTKINIFMSKIEFIFIKHDY", "aa_hexdigest": "5e07262047e1963a15551a5fdd32a152", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000169E514", "ncbi_nrp_id": "WP_006688960.1", "uniref100_id": "UniRef100_A0AAC9X6V1", "db_xrefs": [ "SO:0001217", "UniParc:UPI000169E514", "RefSeq:WP_006688960.1", "UniRef:UniRef100_A0AAC9X6V1" ] }, "ips": { "uniref100_id": "UniRef100_A0AAC9X6V1", "uniref90_id": "UniRef90_A0A2C9DY85" }, "psc": { "uniref90_id": "UniRef90_A0A2C9DY85", "gene": "YIH1", "product": "Impact N-terminal domain-containing protein", "uniref50_id": "UniRef50_A0A2C9DY85", "cog_id": "COG1739", "cog_category": "R" }, "pscc": { "uniref50_id": "UniRef50_A0A2C9DY85", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2C9DY85" ], "product": "Impact N-terminal domain-containing protein" }, "genes": [ "yIH1" ], "id": "NKIECMJPJD_1080", "locus": "GCA000828735_01077" }, { "type": "cds", "sequence": "contig_1", "start": 631576, "stop": 631833, "strand": "-", "frame": 3, "gene": null, "product": "DUF4231 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9X6H3", "UniRef:UniRef90_A0AAC9X6H3" ], "nt": "ATGAACAATTACATACAGCAAGTTATTGATTATGTTAATGATAAAACCTATAAAAATAAGACATATTTTATAAAGAAATATGCATTTGCTTTTTTGACTTCATTAATAATTTTTATTTTTTTATTAGTATTTGGTTTATCAAATTATTTTAATCCTTTGGTTAGTTTAGTTGGTGTTGTATTAATTTTTAGTGTTTTAGTTGTCTTTTGTATTCATATAAAAATTATCAATAATTATAGTTTAAATTTATTAAAATGA", "aa": "MNNYIQQVIDYVNDKTYKNKTYFIKKYAFAFLTSLIIFIFLLVFGLSNYFNPLVSLVGVVLIFSVLVVFCIHIKIINNYSLNLLK", "aa_hexdigest": "400f7ff27682497c1f850f43a5329eb6", "start_type": "ATG", "rbs_motif": "AGxAG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 631426, "stop": 631833, "observations": { "mutation": [ 631578 ], "stop": [ 631578 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAC9X6H3", "query_cov": 1.0, "subject_cov": 0.6296296296296297, "identity": 1.0, "score": 157.0, "evalue": 5.35e-48, "gene_start": 1, "gene_end": 85, "reference_start": 1, "reference_end": 85, "reference_sequence": "MNNYIQQVIDYVNDKTYKNKTYFIKKYAFAFLTSLIIFIFLLVFGLSNYFNPLVSLVGVVLIFSVLVVFCIHIKIINNYSLNLLKWLRNQKELSYKYIINILDFILNKQLYLNLTIIEVQQICIDLEEFKKTNVI" }, "paralog": false, "identity": 0.9925925925925926, "score": 262.0, "evalue": 5.82e-92, "description": "Internal stop codon at 631578. Nonsense mutation around 631578.", "psc": { "uniref90_id": "UniRef90_A0AAC9X6H3", "product": "DUF4231 domain-containing protein", "uniref50_id": "UniRef50_A0AAC9X6H3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAC9X6H3", "UniRef:UniRef50_A0AAC9X6H3" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAC9X6H3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9X6H3" ], "product": "DUF4231 domain-containing protein" } }, "id": "NKIECMJPJD_1081", "locus": "GCA000828735_01078" }, { "type": "cds", "sequence": "contig_1", "start": 631900, "stop": 632070, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTAATGATCATGTAATAAAACAAGAATTTAATTTAGCTAATTATACTGAAAATTCATACGATTACAATGCTGTTAATTTTCATTCACCTCTTGAAATTAAAGTTTATGAAGATCAATTTGGCGGAAAAGCAATTCGTTTTTCATTTGTTGATATTGATCCTAATTAA", "aa": "MFNDHVIKQEFNLANYTENSYDYNAVNFHSPLEIKVYEDQFGGKAIRFSFVDIDPN", "aa_hexdigest": "d2aa4f3fb73b765b17fc08eef5d8abf4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6606.1698000000015, "isoelectric_point": 4.547426414489746 }, "id": "NKIECMJPJD_1082", "locus": "GCA000828735_01079" }, { "type": "cds", "sequence": "contig_1", "start": 632092, "stop": 632403, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTATATCCTGATGATAATTTATTTCAAGAATCGAGCGAGGAAACAGCAGAAGGATTAAAAGATTCAAAAATCAATAATCGTAGTTCTTTTTATCGTGCTCAATCGAATGAATTTATTGATGGGTTATATGTTGTAGAAAGTTCTAATTTAAATAAAAAACCAGAAGAATTAGATGAATATACTGATTTTAGTAAAACAGCTAAATTTACTCTGTTGAATAATGAAGGTTTTGTTTTTACAATCTCCATTGAAGAAAATTATGATAAAACCAAAAAACTAATTTCGCGTAAAGTTAGTTTGTTGCCATGA", "aa": "MLYPDDNLFQESSEETAEGLKDSKINNRSSFYRAQSNEFIDGLYVVESSNLNKKPEELDEYTDFSKTAKFTLLNNEGFVFTISIEENYDKTKKLISRKVSLLP", "aa_hexdigest": "bc54aabfc2040e377a830c65187c3e79", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11918.065399999983, "isoelectric_point": 4.662582206726075 }, "id": "NKIECMJPJD_1083", "locus": "GCA000828735_01080" }, { "type": "cds", "sequence": "contig_1", "start": 632440, "stop": 632592, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCAAAATGATGCTAATAAAGCAGCAGTATTTAATGAAAATACAACTAGAAGTAAACTTAATAACAATCTTTTTAATAATGTATCATTAAGCAATCCATTTGCTTATGAACAAGCACGTAAGTTTCACAATACATTATTTAATAAAAATTGA", "aa": "MQNDANKAAVFNENTTRSKLNNNLFNNVSLSNPFAYEQARKFHNTLFNKN", "aa_hexdigest": "7838c3e1ebfcd90426c76f109461a107", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5791.301700000001, "isoelectric_point": 9.819775962829588 }, "id": "NKIECMJPJD_1084", "locus": "GCA000828735_01081" }, { "type": "cds", "sequence": "contig_1", "start": 632611, "stop": 632721, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATTAATAATCTTTTAATAACTATTTTTCCTAATAAAAAAGACCGTGATGAATATATTAAATCACAACAAGAAATTAAAATAGAATATGCTCATGAAATAGCGAATTGA", "aa": "MINNLLITIFPNKKDRDEYIKSQQEIKIEYAHEIAN", "aa_hexdigest": "4c324ac8d1e1c54a54dbc970d47787ec", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4333.9159, "isoelectric_point": 5.596389961242674 }, "id": "NKIECMJPJD_1085", "locus": "GCA000828735_01082" }, { "type": "cds", "sequence": "contig_1", "start": 632743, "stop": 633195, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTATTTTTTTTAAAAAAAATCATCTACTAAAATTAAGTTTATTTTTTATTCCTCTAACATTAATTTCAATCCTTATCACCTCATGTAGCACATCAAATTTAAAAAAACAACAGATATTAACAAATAATGAAATTAGTTTTTTTATGGATAAATCCCAAAATTGTTATGTTGGTTTAATTGTTGATCAGACATTAAAAAATGATAAAAAAACTATTTTTAACGTTAGTTTAATTGATGAAAAAAATACGAAGTTAAATTTATTAGTTAAAGATTATCAAATTAAAGATAACAAAATTTTAATTAGATTACCGCGTTTAATAAAACTTAATGATCATTTAATTATTACAAGCAATAATGCTTCATTCATACCTATTAGAAAAATAATTAATATTGATAATTTAATTGATTTGAATATAATCAGAACAAATAATCAAACACAAGATTCTTGA", "aa": "MFIFFKKNHLLKLSLFFIPLTLISILITSCSTSNLKKQQILTNNEISFFMDKSQNCYVGLIVDQTLKNDKKTIFNVSLIDEKNTKLNLLVKDYQIKDNKILIRLPRLIKLNDHLIITSNNASFIPIRKIINIDNLIDLNIIRTNNQTQDS", "aa_hexdigest": "13b4d6bd2b258b879b95f67fa7c056e0", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17437.372799999994, "isoelectric_point": 9.628240394592286 }, "id": "NKIECMJPJD_1086", "locus": "GCA000828735_01083" }, { "type": "cds", "sequence": "contig_1", "start": 633211, "stop": 633594, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAGGACCAAGTGAAAAATATTTAAAAAGGTTAAAAAAACTTAAACCATATGAGGATTATTATTTCATTAATAATTATTTAGAAAAAAAACAAGAAGGAGATACTTCAATGTTGATTGGTTCATTTAAGGATTTATATATTACAAAAAATCAATCTATTTTTAATATTAAGATCGATTTTTCAAGTAGTGAAAACAGATTAAGTTTAAATCCTTTGATTTATTTTTTTCCTAAAACTAAAAATAAATTATCAGTTGATTTAATCACATCAATTTTTCATCAAGCTATATTTCACCAAAATAAAGAATACTATCAATTGTTTGAAACTGATATGATTGATAAATATGAATATGGTATCCCAGCCCAAATGTTACTTTTATGA", "aa": "MLGPSEKYLKRLKKLKPYEDYYFINNYLEKKQEGDTSMLIGSFKDLYITKNQSIFNIKIDFSSSENRLSLNPLIYFFPKTKNKLSVDLITSIFHQAIFHQNKEYYQLFETDMIDKYEYGIPAQMLLL", "aa_hexdigest": "f3f9604ec496aa79b5269033fb9e5502", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15191.396299999988, "isoelectric_point": 8.614668464660646 }, "id": "NKIECMJPJD_1087", "locus": "GCA000828735_01084" }, { "type": "cds", "sequence": "contig_1", "start": 633634, "stop": 634476, "strand": "-", "frame": 3, "gene": null, "product": "Lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2C9DYW8", "UniRef:UniRef90_A0A2C9DYW8" ], "nt": "TTGCAATTTGGTGATCACTTAATCTTACGTTTATTAAAATTTAATAAAAAAAATCATTTTGATGTTCGATTTGATACAGATCTTTTTCAATTCAATAAACCAATTATAAATTTGGAATTAATAGCAAAAGAAATGCAAACAAGTATTGATTATCATCGAGAAGATAATTTTAAAAAAGATATTTTAGATTATAAAAAAAATTATGAAGATTATTTATATTATGATGAAAAGCTTAACTCAAGTATTTATCAACAGGGATTAGATAAAATAAATAAAGAATATTATGAAGTCAATAATTTTAATTTTTTTAATACTAAAAAACCACTTTTTCAAAAATTTAGTTATCAAATGTTTTTAGAATTAAAAGATAAGTTGGGTTTTAAACGTTATGTTTTAAGAAATATTGATCAAAAAGAAGTTAGAGCACGTGATCATTTTAATGACAATGAAAAAAAACAACAAAATAAGAATTATTTTAGATCTTTTGATAAAGAATTGAATTATTTTCAAAAATATATTGATCATAATTTAGATATTAATATTAATAAAATTTCAAAGGAAGCATATATTACACAAAAAATAATTAAAAATCTTTTATTAGATGCTTTCAATAATAATGATGTTCAAATTACAAAATATCTAATTCAACAAAATGATAAGGAATACCAAAATAAATTAATTAAACAATTAATAGAATTTAGTAATTATTTTAAAAACCAAAAAACACCAAGAGATTCTAATGAATATAAGCAAAAATTAAATGATTATGAAAAAATATTTTCACAAAATTGGTATTTTATTTTAATGAATTTAAAGCACTTTGAATTAAGTTTTAATAATTGA", "aa": "MQFGDHLILRLLKFNKKNHFDVRFDTDLFQFNKPIINLELIAKEMQTSIDYHREDNFKKDILDYKKNYEDYLYYDEKLNSSIYQQGLDKINKEYYEVNNFNFFNTKKPLFQKFSYQMFLELKDKLGFKRYVLRNIDQKEVRARDHFNDNEKKQQNKNYFRSFDKELNYFQKYIDHNLDININKISKEAYITQKIIKNLLLDAFNNNDVQITKYLIQQNDKEYQNKLIKQLIEFSNYFKNQKTPRDSNEYKQKLNDYEKIFSQNWYFILMNLKHFELSFNN", "aa_hexdigest": "e920b36545e522cce1e63568d2e0a5df", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 633331, "stop": 634776, "observations": { "mutation": [ 633636, 634686, 634692 ], "stop": [ 633636, 634686, 634692 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A2C9DYW8", "query_cov": 1.0, "subject_cov": 0.449438202247191, "identity": 0.996, "score": 548.0, "evalue": 1.23e-191, "gene_start": 1, "gene_end": 280, "reference_start": 198, "reference_end": 477, "reference_sequence": "MNKQKRKISVLTSISLFLIISLSIGLGLGLIKNNENSITSKVQKSSNFFINDLSKQLFLDVFNQDKLELNAYMYDQNNVSDQQLLDLKFALTLLNPNRLDINDDDTIAIMKNNAKKIIFQSLSNNWYWLFTNLKSLEFSYTPYKPNFYAPYINEKQDFTKSSIFDKVININKPLTNYIKIKQKLFDKENYRSVDVYYLQFGDHLILRLLKFNKKNHFDVRFDTDLFQFNKPIINLELIAKEMQTSIDYHREDNFKKDILDYKKNYEDYLYYDEKLNSSIYQQGLDKINKEYYEVNNFNFFNTKKPLFQKFSYQMFLELKDKLGFKRYVLRNIDQKEVRARDHFNDNEKKQQNKNYFRSFDKELNYFQKYIDHNLDININKISKEAYITQKIIKNLLLDAFNNNDVQITKYLIQQNDKEYQNKLIKQLIEFSNYFKNQKTPRDSNEYKQKLNDYEKIFSQNWYFILMNLKHFELSFNNWFTLPKQINPITKEMLGPSEKYLKRLKKLKPYEDYYFINNYLEKKQEGDTSMLIGSFKDLYITKNQSIFNIKIDFSSSENRLSLNPLIYFFPKTKNKLSVDLITSIFHQAIFHQNKEYYQLFETDMIDKYEYGIPAQCYFYEGNSN" }, "paralog": false, "identity": 0.9937629937629938, "score": 969.0, "evalue": 0.0, "description": "Internal stop codon at 633636, 634686, 634692. Nonsense mutation around 633636, 634686, 634692.", "psc": { "uniref90_id": "UniRef90_A0A2C9DYW8", "product": "Lipoprotein", "uniref50_id": "UniRef50_A0A2C9DYW8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A2C9DYW8", "UniRef:UniRef50_A0A2C9DYW8" ] }, "pscc": { "uniref50_id": "UniRef50_A0A2C9DYW8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2C9DYW8" ], "product": "Lipoprotein" } }, "id": "NKIECMJPJD_1088", "locus": "GCA000828735_01085" }, { "type": "cds", "sequence": "contig_1", "start": 634690, "stop": 635067, "strand": "-", "frame": 3, "gene": null, "product": "aromatic motif membrane protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E28CBB8", "UniRef:UniRef90_UPI001E28CBB8" ], "nt": "ATGAATAAACAAAAAAGAAAAATCTCAGTATTAACTTCAATATCATTATTTTTAATTATTAGTTTAAGTATTGGGTTAGGTTTGGGATTAATAAAAAATAATGAAAATTCCATCACTTCTAAAGTTCAAAAAAGTAGCAATTTTTTTATTAATGATTTATCAAAACAATTGTTTTTAGATGTTTTTAATCAAGATAAATTAGAATTAAATGCTTATATGTATGATCAAAATAATGTATCAGATCAACAATTATTAGATTTAAAATTTGCTTTAACATTATTAAATCCTAACAGATTAGATATTAATGATGACGATACAATTGCCATTATGAAAAATAATGCTAAAAAAATTATTTTTCAAAGTTTATCTAATAATTGA", "aa": "MNKQKRKISVLTSISLFLIISLSIGLGLGLIKNNENSITSKVQKSSNFFINDLSKQLFLDVFNQDKLELNAYMYDQNNVSDQQLLDLKFALTLLNPNRLDINDDDTIAIMKNNAKKIIFQSLSNN", "aa_hexdigest": "d2e5c49cfa2d17e372ed4c72789b8176", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 634389, "stop": 635067, "observations": { "insertion": [ 634431 ], "mutation": [ 634686, 634692 ], "stop": [ 634686, 634692 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E28CBB8", "query_cov": 1.0, "subject_cov": 0.5387931034482759, "identity": 1.0, "score": 204.0, "evalue": 1.62e-64, "gene_start": 1, "gene_end": 125, "reference_start": 1, "reference_end": 125, "reference_sequence": "MNKQKRKISVLTSISLFLIISLSIGLGLGLIKNNENSITSKVQKSSNFFINDLSKQLFLDVFNQDKLELNAYMYDQNNVSDQQLLDLKFALTLLNPNRLDINDDDTIAIMKNNAKKIIFQSLSNNWYWLFTNLKSLEFSYTPYKPNFYAPYINEKQDFTKSSIFDKVININKPLTNYIKIKQKLFDKENYRSVDVYYLQFGDHLILRLLKFNKKIILMFDLIQIFFNSINQL" }, "paralog": false, "identity": 0.9911111111111112, "score": 397.0, "evalue": 4.54e-143, "description": "Internal stop codon at 634686, 634692. Frameshift due to insertion around 634431. Nonsense mutation around 634686, 634692.", "psc": { "uniref90_id": "UniRef90_UPI001E28CBB8", "product": "aromatic motif membrane protein", "uniref50_id": "UniRef50_UPI001E28CBB8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E28CBB8", "UniRef:UniRef50_UPI001E28CBB8" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E28CBB8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E28CBB8" ], "product": "aromatic motif membrane protein" } }, "id": "NKIECMJPJD_1089", "locus": "GCA000828735_01086" }, { "type": "cds", "sequence": "contig_1", "start": 635334, "stop": 635648, "strand": "-", "frame": 1, "gene": "rplQ", "product": "50S ribosomal protein L17", "db_xrefs": [ "COG:COG0203", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625", "KEGG:K02879", "SO:0001217", "UniRef:UniRef50_P47424", "UniRef:UniRef90_Q9PQN3" ], "nt": "ATGGTGAGTCGTCAACAAGTATCTGATGTTATAAGTCATGGTTCAATTGTGACAACAAAAACAAAAGCAAAAGAATCACAACGTCATGTTGATCATTTAATCACTCTTGCTAAGAAAAACACACTTGCTTCACGTCGTGCAGCAGCTGCGATTTTATTAGGAACAAATCAACACTCAGCGGATGATTTATTACGTAAATTATTTAACGAATTAGGTCCTAAGTATGCTAATCGTGCTGGTGGATACACTCGTGTTATTAAACTTGGTAATCGTCCAGGAGATAATACAGAAGAAGCTGTTTTACAATTAGTTTAG", "aa": "MVSRQQVSDVISHGSIVTTKTKAKESQRHVDHLITLAKKNTLASRRAAAAILLGTNQHSADDLLRKLFNELGPKYANRAGGYTRVIKLGNRPGDNTEEAVLQLV", "aa_hexdigest": "5bdcf156fcc3b61db1024f562859be8f", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_Q9PQN3", "query_cov": 1.0, "subject_cov": 0.8739495798319328, "identity": 1.0, "score": 200.0, "evalue": 8.47e-65, "valid": true, "gene": "rplQ", "product": "50S ribosomal protein L17", "uniref50_id": "UniRef50_P47424", "cog_id": "COG0203", "cog_category": "J", "kegg_orthology_id": "K02879", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_P47424", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47424" ], "product": "Large ribosomal subunit protein bL17" }, "genes": [ "rplQ" ], "id": "NKIECMJPJD_1090", "locus": "GCA000828735_01087" }, { "type": "cds", "sequence": "contig_1", "start": 635721, "stop": 636374, "strand": "-", "frame": 1, "gene": "rpoA", "product": "DNA-directed RNA polymerase subunit alpha", "db_xrefs": [ "COG:COG0202", "COG:K", "EC:2.7.7.6", "GO:0000428", "GO:0001054", "GO:0001055", "GO:0001056", "GO:0001057", "GO:0001058", "GO:0001066", "GO:0003677", "GO:0003899", "GO:0005737", "GO:0006351", "GO:0046983", "SO:0001217", "UniRef:UniRef50_Q9PQN4", "UniRef:UniRef90_Q9PQN4" ], "nt": "ATGAAAATTAATTTCAAAGGACCTGGTGTGATTACAGCTAAAGATATTGTATGTCCAGTAGGTTTTGAAATTGTTAACCAAGACTTATATATTGCAGAAGTAACAAAACCAATTGATGTAGAAATTGAAATTTTTGCAAAAACAGGACGTGGTCGTGTCGATTTTAATACAAATAAGGATTTTGTTTCAACACTACATATTATCGCAACCGATTCAAATTATTCACCAGTTCTACATTACGCTTATAATGTAGAAATGATTAAAGATTCAAAATCATCAATGTCTGAGATTTTAACAATTGACATTGCAACAAATGGTACGATATCAGGAAGCGAAGCAATTGCGATTGCTGCTAAGATTATGCAAGCGCATTTAGAACCAATCGTGAACATTGATAAAACAATTAATGAAATGATTATTATGCGTGAGCGTGAGGAAGAAGAAAAACGTCAAAACGCTTCAATTTCAATTGATGATTTAGATTTAACGGTGCGTGCATATAATGCTTTAAAACAATCAGGTATTAATACCACTGCTGAATTAATTGAATTAACAAAATCACAACTTGAAAAAATTAAGAATTTAGGACGTAAATCAGTTACTGAAATTATTCAAAAATTAACTGAACGTTCACTTGAGTTAAAAAAAGATTAA", "aa": "MKINFKGPGVITAKDIVCPVGFEIVNQDLYIAEVTKPIDVEIEIFAKTGRGRVDFNTNKDFVSTLHIIATDSNYSPVLHYAYNVEMIKDSKSSMSEILTIDIATNGTISGSEAIAIAAKIMQAHLEPIVNIDKTINEMIIMREREEEEKRQNASISIDDLDLTVRAYNALKQSGINTTAELIELTKSQLEKIKNLGRKSVTEIIQKLTERSLELKKD", "aa_hexdigest": "9ad8f1f32d9374f340e0dd7a40f78f74", "start_type": "ATG", "rbs_motif": null, "genes": [ "rpoA" ], "truncated": "3-prime", "pseudogene": { "start": 635721, "stop": 636674, "observations": { "mutation": [ 636383 ], "stop": [ 636383 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQN4", "query_cov": 1.0, "subject_cov": 0.6636085626911316, "identity": 1.0, "score": 406.0, "evalue": 3.32e-141, "gene_start": 1, "gene_end": 217, "reference_start": 111, "reference_end": 327, "reference_sequence": "MRKFLKYQLDVPSINSEDKNRTVVKIAPLEIGFGDTLGNALRRICLSSIPGASMFAVKFGGYSHEFQPYEGVKEDITHIILNLKNLAIKIDELIYSEDYFNNLLIDKWPKMKINFKGPGVITAKDIVCPVGFEIVNQDLYIAEVTKPIDVEIEIFAKTGRGRVDFNTNKDFVSTLHIIATDSNYSPVLHYAYNVEMIKDSKSSMSEILTIDIATNGTISGSEAIAIAAKIMQAHLEPIVNIDKTINEMIIMREREEEEKRQNASISIDDLDLTVRAYNALKQSGINTTAELIELTKSQLEKIKNLGRKSVTEIIQKLTERSLELKKD" }, "paralog": false, "identity": 0.9968454258675079, "score": 611.0, "evalue": 1.65e-224, "description": "Internal stop codon at 636383. Nonsense mutation around 636383.", "psc": { "uniref90_id": "UniRef90_Q9PQN4", "gene": "rpoA", "product": "DNA-directed RNA polymerase subunit alpha", "ec_ids": [ "2.7.7.6" ], "uniref50_id": "UniRef50_Q9PQN4", "cog_id": "COG0202", "cog_category": "K", "go_ids": [ "GO:0000428", "GO:0001054", "GO:0001055", "GO:0001056", "GO:0001057", "GO:0001058", "GO:0001066", "GO:0003677", "GO:0003899", "GO:0005737", "GO:0006351", "GO:0046983" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQN4", "EC:2.7.7.6", "UniRef:UniRef50_Q9PQN4", "COG:COG0202", "COG:K", "GO:0000428", "GO:0001054", "GO:0001055", "GO:0001056", "GO:0001057", "GO:0001058", "GO:0001066", "GO:0003677", "GO:0003899", "GO:0005737", "GO:0006351", "GO:0046983" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQN4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQN4" ], "product": "DNA-directed RNA polymerase subunit alpha" } }, "id": "NKIECMJPJD_1091", "locus": "GCA000828735_01088" }, { "type": "cds", "sequence": "contig_1", "start": 636381, "stop": 636704, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01193.29" ], "nt": "ATGCGTAAATTTTTAAAATACCAACTTGATGTGCCATCAATTAATAGCGAAGACAAAAATCGTACAGTTGTTAAAATTGCCCCACTTGAAATTGGTTTTGGTGACACATTAGGAAATGCCTTACGTCGAATTTGTTTATCATCAATTCCTGGTGCCTCAATGTTCGCTGTTAAATTTGGGGGATATTCGCATGAATTTCAACCATACGAAGGTGTTAAAGAAGACATTACTCACATCATTTTAAACTTAAAAAATTTAGCAATTAAAATTGATGAATTAATTTATAGTGAAGACTATTTCAACAATTTATTAATTGATAAATGA", "aa": "MRKFLKYQLDVPSINSEDKNRTVVKIAPLEIGFGDTLGNALRRICLSSIPGASMFAVKFGGYSHEFQPYEGVKEDITHIILNLKNLAIKIDELIYSEDYFNNLLIDK", "aa_hexdigest": "78b9b1fc7bd3960ded08d074ca95e8d8", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_A0A8S0FJM5", "query_cov": 0.822429906542056, "subject_cov": 0.9166666666666666, "identity": 0.5, "score": 80.5, "evalue": 1.06e-17, "valid": false, "product": "DNA-directed RNA polymerase subunit alpha", "uniref50_id": "UniRef50_A0A3P8JBX3" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01193.29", "name": "RNA_pol_L", "length": 68, "aa_cov": 0.616822429906542, "hmm_cov": 0.8970588235294118, "evalue": 1.0242845031042905e-11, "score": 40.00569534301758, "start": 28, "stop": 93 } ], "seq_stats": { "molecular_weight": 12166.917099999988, "isoelectric_point": 6.081681251525878 }, "id": "NKIECMJPJD_1092", "locus": "GCA000828735_01089" }, { "type": "cds", "sequence": "contig_1", "start": 636741, "stop": 636899, "strand": "-", "frame": 1, "gene": "rpsK", "product": "30S ribosomal protein S11", "db_xrefs": [ "COG:COG0100", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904", "KEGG:K02948", "SO:0001217", "UniRef:UniRef50_Q46451", "UniRef:UniRef90_Q9PQN5" ], "nt": "ATGGGATTAAAATCTGTTGAAGTTCATGTTAATGGAACAGGTGCAAGTCGTGATACAGCAATTCGTAGCCTACAAGCAGCAGGTTTAGAAGTAACAAAAATTAAAGATGTTACTCCAATTCCACATAATGGGTGTCGTCCACCTAAAAAACCAAGATAG", "aa": "MGLKSVEVHVNGTGASRDTAIRSLQAAGLEVTKIKDVTPIPHNGCRPPKKPR", "aa_hexdigest": "c8589cc617200bf5133cdf178b883ee6", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI003341A311", "query_cov": 0.9615384615384616, "subject_cov": 0.9803921568627451, "identity": 0.68, "score": 75.9, "evalue": 2.6e-17, "valid": false, "product": "30S ribosomal protein S11", "uniref50_id": "UniRef50_A0A1V5HTP7" }, "genes": [ "rpsK" ], "truncated": "3-prime", "pseudogene": { "start": 636741, "stop": 637106, "observations": { "mutation": [ 636998 ], "stop": [ 636998 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQN5", "query_cov": 1.0, "subject_cov": 0.4297520661157025, "identity": 1.0, "score": 108.0, "evalue": 2.7e-29, "gene_start": 1, "gene_end": 52, "reference_start": 70, "reference_end": 121, "reference_sequence": "MAKKKKLSFTNGIAYIHATKNNTIITLADEQGSVLSWASSGSIGYKGTKKKTPYSAGIAAEAAAKAVIDMGLKSVEVHVNGTGASRDTAIRSLQAAGLEVTKIKDVTPIPHNGCRPPKKPR" }, "paralog": false, "identity": 0.9917355371900827, "score": 235.0, "evalue": 6.85e-82, "description": "Internal stop codon at 636998. Nonsense mutation around 636998.", "psc": { "uniref90_id": "UniRef90_Q9PQN5", "gene": "rpsK", "product": "30S ribosomal protein S11", "uniref50_id": "UniRef50_Q46451", "cog_id": "COG0100", "cog_category": "J", "kegg_orthology_id": "K02948", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQN5", "UniRef:UniRef50_Q46451", "COG:COG0100", "COG:J", "KEGG:K02948", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_Q46451", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q46451" ], "product": "Small ribosomal subunit protein uS11" } }, "id": "NKIECMJPJD_1093", "locus": "GCA000828735_01090" }, { "type": "cds", "sequence": "contig_1", "start": 636996, "stop": 637106, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAAAGAAGAAAAAACTTAGTTTTACTAATGGAATTGCATATATTCATGCAACTAAAAATAATACGATTATTACTTTGGCTGATGAACAAGGAAGCGTTTTATCTTGA", "aa": "MAKKKKLSFTNGIAYIHATKNNTIITLADEQGSVLS", "aa_hexdigest": "f49a0d83f0b36385a1968d9643fa6c10", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3907.4934000000003, "isoelectric_point": 9.699671363830568 }, "id": "NKIECMJPJD_1094", "locus": "GCA000828735_01091" }, { "type": "cds", "sequence": "contig_1", "start": 637128, "stop": 637526, "strand": "-", "frame": 1, "gene": "rpsM", "product": "30S ribosomal protein S13", "db_xrefs": [ "COG:COG0099", "COG:J", "GO:0000049", "GO:0003723", "GO:0003735", "GO:0005829", "GO:0005840", "GO:0006412", "GO:0015935", "GO:0019843", "RefSeq:WP_006688863.1", "SO:0001217", "UniParc:UPI00000C1BEC", "UniRef:UniRef100_Q9PQN6", "UniRef:UniRef50_Q9PQN6", "UniRef:UniRef90_Q9PQN6" ], "nt": "ATGGCACGTATTTTAGGGGTAGATATTCCTAATGATAAAAGAGTTGTAATTTCATTAACATACATTTTTGGTATTGGAAAATCTACATCTCAACAAATTTTAAAATTAGCTAATATAGATGAAAATATTCGTGTAAAGGATTTAGCTGATGAACAAATTGCTGAAATTCGTCGAGTTGCTTTAAATTTTGTTAAAGCAAATGGCGAAAAATTACAATTAGAAGGTGATTTACGAAGAACTGTAGCCATGGATATTAAGCGTTTAATGGAAATCGGTTCATATCGTGGAATCCGTCACCGTCGTGGTTTACCAGTGCGTGGACAAAGAACAAAAACAAACGCTCGTACACGTAAAGGACCACGTAAAACAGTTGCTAATAAAAAGATTGAAACTAGATAA", "aa": "MARILGVDIPNDKRVVISLTYIFGIGKSTSQQILKLANIDENIRVKDLADEQIAEIRRVALNFVKANGEKLQLEGDLRRTVAMDIKRLMEIGSYRGIRHRRGLPVRGQRTKTNARTRKGPRKTVANKKIETR", "aa_hexdigest": "f0b55fcf5b9f591bcb04bff715717f28", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "ups": { "uniparc_id": "UPI00000C1BEC", "ncbi_nrp_id": "WP_006688863.1", "uniref100_id": "UniRef100_Q9PQN6", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1BEC", "RefSeq:WP_006688863.1", "UniRef:UniRef100_Q9PQN6" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQN6", "uniref90_id": "UniRef90_Q9PQN6" }, "psc": { "uniref90_id": "UniRef90_Q9PQN6", "gene": "rpsM", "product": "30S ribosomal protein S13", "uniref50_id": "UniRef50_Q9PQN6", "cog_id": "COG0099", "cog_category": "J", "go_ids": [ "GO:0000049", "GO:0003723", "GO:0003735", "GO:0005829", "GO:0005840", "GO:0006412", "GO:0015935", "GO:0019843" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQN6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQN6" ], "product": "Small ribosomal subunit protein uS13" }, "genes": [ "rpsM" ], "id": "NKIECMJPJD_1095", "locus": "GCA000828735_01092" }, { "type": "cds", "sequence": "contig_1", "start": 637551, "stop": 637664, "strand": "-", "frame": 1, "gene": "rpmJ", "product": "50S ribosomal protein L36", "db_xrefs": [ "COG:COG0257", "COG:J", "GO:0003735", "GO:0005737", "GO:0005840", "GO:0006412", "GO:1990904", "RefSeq:WP_006688931.1", "SO:0001217", "UniParc:UPI0000134041", "UniRef:UniRef100_Q9PQN7", "UniRef:UniRef50_Q85FI9", "UniRef:UniRef90_Q9PQN7" ], "nt": "ATGAAAGTTAGAGCTTCAGTTAAAGCAATTTGTAAAGATTGCAAAATCGTAAAGCGATCAGGTGTTGTGCGTGTTATTTGTGCTAATTCAAAACACAAACAACGTCAAGGTTAA", "aa": "MKVRASVKAICKDCKIVKRSGVVRVICANSKHKQRQG", "aa_hexdigest": "d28a9c087bfea917966c78080d770074", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0000134041", "ncbi_nrp_id": "WP_006688931.1", "uniref100_id": "UniRef100_Q9PQN7", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000134041", "RefSeq:WP_006688931.1", "UniRef:UniRef100_Q9PQN7" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQN7", "uniref90_id": "UniRef90_Q9PQN7" }, "psc": { "uniref90_id": "UniRef90_Q9PQN7", "gene": "rpmJ", "product": "50S ribosomal protein L36", "uniref50_id": "UniRef50_Q85FI9", "cog_id": "COG0257", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005737", "GO:0005840", "GO:0006412", "GO:1990904" ] }, "genes": [ "rpmJ" ], "id": "NKIECMJPJD_1096", "locus": "GCA000828735_01093" }, { "type": "cds", "sequence": "contig_1", "start": 637688, "stop": 637912, "strand": "-", "frame": 2, "gene": "infA", "product": "translation initiation factor IF-1", "db_xrefs": [ "COG:COG0361", "COG:J", "GO:0003743", "GO:0005829", "GO:0006413", "GO:0019843", "GO:0043022", "KEGG:K02518", "RefSeq:WP_006688844.1", "SO:0001217", "UniParc:UPI000012D274", "UniRef:UniRef100_Q9PQN8", "UniRef:UniRef50_Q6F1X1", "UniRef:UniRef90_Q9PQN8" ], "nt": "ATGGCAGATACAGAAAAATTAAAAATGCTTGGTAAAATTGTTGAAGTTTTACAAGGCGGAAATTTTAGAGTTCAATTAGAAAATGGCATCACGATTATGAGCCACGTATCAGGTAAAATGCGTGTTAATAAAATTAACATTTTACCAGGTGATACTGTTGATGTTGAGTTAAGTCCATACGACCTAACACGTGGAAGAATTACATATCGTCATCGTGATAACTAA", "aa": "MADTEKLKMLGKIVEVLQGGNFRVQLENGITIMSHVSGKMRVNKINILPGDTVDVELSPYDLTRGRITYRHRDN", "aa_hexdigest": "c8a6615a3951a4741a7bcdb746d716b3", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI000012D274", "ncbi_nrp_id": "WP_006688844.1", "uniref100_id": "UniRef100_Q9PQN8", "db_xrefs": [ "SO:0001217", "UniParc:UPI000012D274", "RefSeq:WP_006688844.1", "UniRef:UniRef100_Q9PQN8" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQN8", "uniref90_id": "UniRef90_Q9PQN8" }, "psc": { "uniref90_id": "UniRef90_Q9PQN8", "gene": "infA", "product": "translation initiation factor IF-1", "uniref50_id": "UniRef50_Q6F1X1", "cog_id": "COG0361", "cog_category": "J", "kegg_orthology_id": "K02518", "go_ids": [ "GO:0003743", "GO:0005829", "GO:0006413", "GO:0019843", "GO:0043022" ] }, "pscc": { "uniref50_id": "UniRef50_Q6F1X1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q6F1X1" ], "product": "Translation initiation factor IF-1" }, "genes": [ "infA" ], "id": "NKIECMJPJD_1097", "locus": "GCA000828735_01094" }, { "type": "cds", "sequence": "contig_1", "start": 638030, "stop": 638479, "strand": "-", "frame": 2, "gene": "map", "product": "type I methionyl aminopeptidase", "db_xrefs": [ "COG:COG0024", "COG:J", "EC:3.4.11.18", "GO:0006508", "GO:0070006", "KEGG:K01265", "SO:0001217", "UniRef:UniRef50_Q9PQN9", "UniRef:UniRef90_Q9PQN9" ], "nt": "GTGAATGAGTGCGTTATTCATGGAGTCCCAACCGATTACGTCTTAAAAGATGGTGATAAGGTAACATTTGATGTTGGTGTTAAATACGATAATCATTATTGTGATGCGGCGTTTACCATTATTATTAACAACTCAAATGTTGAAGCACTAAAAATATCAGAGATTTGTAAAAAATCAATTGACGAAGCCGTTGCAATTATTAAACCAAAAGTAACAACACATGCAATTTCGAATGCAATTCAAAAGTTTATTGAAAAAAACGGTTATTTTGTTCTTCGTGATTTTGCCGGCCACGGTTGTGGTAATGAAATTCATGAAGATCCATTGATTCCTAATTATCGATCTTTATTGTATCGTAATGTTACATTAGAAGAGAATATGGTAATTTGTATTGAACCAATGATTCTAAGTGGATCAAATGCTTATTATATTGATCCAAACGATCAATGA", "aa": "MNECVIHGVPTDYVLKDGDKVTFDVGVKYDNHYCDAAFTIIINNSNVEALKISEICKKSIDEAVAIIKPKVTTHAISNAIQKFIEKNGYFVLRDFAGHGCGNEIHEDPLIPNYRSLLYRNVTLEENMVICIEPMILSGSNAYYIDPNDQ", "aa_hexdigest": "281e369c295f74129be37c20983a0a0d", "start_type": "GTG", "rbs_motif": null, "genes": [ "map" ], "truncated": "both", "pseudogene": { "start": 637943, "stop": 638689, "observations": { "mutation": [ 637993, 638032, 638632 ], "stop": [ 637993, 638032, 638632 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQN9", "query_cov": 1.0, "subject_cov": 0.5983935742971888, "identity": 0.966, "score": 304.0, "evalue": 3.06e-103, "gene_start": 1, "gene_end": 149, "reference_start": 71, "reference_end": 219, "reference_sequence": "MVIVKTEKDITAIKEAVRIWKIAREAIYEQVKAGVSLKELDLLAKEVIEANGGIAAFHNYLGFKGHICISVNECVIHGVPTDYILKDGDKVTFDVGVKYDNHYCDAAFTIIINNSNVEALKMSEICKKSIDEAVAIIKPKVTTHAISNVIQKFIEKNGYFILRDFAGHGCGNEIHEDPLIPNYRSLLYRNVTLEENMVICIEPMILSGSNAYYIDPNDQWSVKSKNHQMTCHWEHMILITKDGCEVLTA" }, "paralog": false, "identity": 0.9596774193548387, "score": 482.0, "evalue": 4.83e-176, "description": "Internal stop codon at 637993, 638032, 638632. Nonsense mutation around 637993, 638032, 638632.", "psc": { "uniref90_id": "UniRef90_Q9PQN9", "gene": "map", "product": "type I methionyl aminopeptidase", "ec_ids": [ "3.4.11.18" ], "uniref50_id": "UniRef50_Q9PQN9", "cog_id": "COG0024", "cog_category": "J", "kegg_orthology_id": "K01265", "go_ids": [ "GO:0006508", "GO:0070006" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQN9", "EC:3.4.11.18", "UniRef:UniRef50_Q9PQN9", "COG:COG0024", "COG:J", "KEGG:K01265", "GO:0006508", "GO:0070006" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQN9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQN9" ], "product": "Methionine aminopeptidase" } }, "id": "NKIECMJPJD_1098", "locus": "GCA000828735_01095" }, { "type": "cds", "sequence": "contig_1", "start": 638714, "stop": 638980, "strand": "-", "frame": 2, "gene": "adk", "product": "Adenylate kinase", "db_xrefs": [ "COG:COG0563", "COG:F", "EC:2.7.4.3", "GO:0004017", "GO:0005524", "GO:0005737", "GO:0008270", "GO:0043231", "GO:0044209", "SO:0001217", "UniRef:UniRef50_Q9PQP0", "UniRef:UniRef90_Q9PQP0" ], "nt": "ATGTGCCCAAAATGTGCAGGAATTTATAATATTCATTTTAAAAAACCACAAGTTCATGGTTTATGTGACAATGATCAAGCTACTCTTTATCAACGAGCTGATGATCATGCAGATGCTGTAAGTATTCGATTAGATGAATATGATAAATTAACCCTACCTTTGATTAAGCATTATGAAACTAATCCACGTTTTATTAAAATCAATGCTAATCAACCTATTGAAGATGTTTATAAAAACATTAACAATTATTTAAAACAAAATAAGTAA", "aa": "MCPKCAGIYNIHFKKPQVHGLCDNDQATLYQRADDHADAVSIRLDEYDKLTLPLIKHYETNPRFIKINANQPIEDVYKNINNYLKQNK", "aa_hexdigest": "a2c5862ecc8f11c219dfb21e321576fb", "start_type": "ATG", "rbs_motif": null, "genes": [ "adk" ], "truncated": "3-prime", "pseudogene": { "start": 638714, "stop": 639280, "observations": { "mutation": [ 638983 ], "stop": [ 638983 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQP0", "query_cov": 1.0, "subject_cov": 0.4131455399061033, "identity": 1.0, "score": 188.0, "evalue": 4.67e-59, "gene_start": 1, "gene_end": 88, "reference_start": 126, "reference_end": 213, "reference_sequence": "MKILLIGPPGSGKGSVSELLTKNNTLKHVSTGNLFRAILKEDSELARKIKEINVSGGKLVPDEITNQVAKSAIDELIKNEQSFILDGYPRTINQALALEQYCNLDYIFYLDIDHQELMKRLTGRWMCPKCAGIYNIHFKKPQVHGLCDNDQATLYQRADDHADAVSIRLDEYDKLTLPLIKHYETNPRFIKINANQPIEDVYKNINNYLKQNK" }, "paralog": false, "identity": 0.9946808510638298, "score": 381.0, "evalue": 1.39e-137, "description": "Internal stop codon at 638983. Nonsense mutation around 638983.", "psc": { "uniref90_id": "UniRef90_Q9PQP0", "gene": "adk", "product": "Adenylate kinase", "ec_ids": [ "2.7.4.3" ], "uniref50_id": "UniRef50_Q9PQP0", "cog_id": "COG0563", "cog_category": "F", "go_ids": [ "GO:0005737", "GO:0043231", "GO:0004017", "GO:0005524", "GO:0008270", "GO:0044209" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQP0", "EC:2.7.4.3", "UniRef:UniRef50_Q9PQP0", "COG:COG0563", "COG:F", "GO:0005737", "GO:0043231", "GO:0004017", "GO:0005524", "GO:0008270", "GO:0044209" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQP0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQP0" ], "product": "Adenylate kinase" } }, "id": "NKIECMJPJD_1099", "locus": "GCA000828735_01096" }, { "type": "cds", "sequence": "contig_1", "start": 638981, "stop": 639355, "strand": "-", "frame": 2, "gene": "adk", "product": "Adenylate kinase", "db_xrefs": [ "COG:COG0563", "COG:F", "EC:2.7.4.3", "GO:0004017", "GO:0005524", "GO:0005737", "GO:0008270", "GO:0043231", "GO:0044209", "SO:0001217", "UniRef:UniRef50_Q9PQP0", "UniRef:UniRef90_Q9PQP0" ], "nt": "ATGAAAATCCTTTTAATTGGCCCCCCAGGGAGTGGTAAGGGAAGTGTTAGTGAATTATTAACTAAAAATAATACATTAAAACATGTTTCCACAGGTAATTTATTTCGTGCAATTCTAAAAGAGGATAGCGAATTAGCACGTAAAATTAAAGAGATTAATGTTTCTGGTGGTAAATTAGTACCAGATGAAATTACTAATCAAGTTGCTAAATCAGCAATTGATGAATTAATTAAAAATGAACAATCATTTATTTTAGATGGTTATCCACGCACAATTAATCAAGCCTTAGCATTAGAACAATATTGTAATTTAGATTATATTTTTTATCTAGATATTGATCATCAAGAATTAATGAAACGTTTAACTGGGCGTTGA", "aa": "MKILLIGPPGSGKGSVSELLTKNNTLKHVSTGNLFRAILKEDSELARKIKEINVSGGKLVPDEITNQVAKSAIDELIKNEQSFILDGYPRTINQALALEQYCNLDYIFYLDIDHQELMKRLTGR", "aa_hexdigest": "f6548b976425739eb0ad0609b0cd0943", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "genes": [ "adk" ], "truncated": "5-prime", "pseudogene": { "start": 638714, "stop": 639355, "observations": { "mutation": [ 638983 ], "stop": [ 638983 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQP0", "query_cov": 1.0, "subject_cov": 0.5821596244131455, "identity": 1.0, "score": 245.0, "evalue": 6.39e-81, "gene_start": 1, "gene_end": 124, "reference_start": 1, "reference_end": 124, "reference_sequence": "MKILLIGPPGSGKGSVSELLTKNNTLKHVSTGNLFRAILKEDSELARKIKEINVSGGKLVPDEITNQVAKSAIDELIKNEQSFILDGYPRTINQALALEQYCNLDYIFYLDIDHQELMKRLTGRWMCPKCAGIYNIHFKKPQVHGLCDNDQATLYQRADDHADAVSIRLDEYDKLTLPLIKHYETNPRFIKINANQPIEDVYKNINNYLKQNK" }, "paralog": false, "identity": 0.9953051643192489, "score": 429.0, "evalue": 5.14e-156, "description": "Internal stop codon at 638983. Nonsense mutation around 638983.", "psc": { "uniref90_id": "UniRef90_Q9PQP0", "gene": "adk", "product": "Adenylate kinase", "ec_ids": [ "2.7.4.3" ], "uniref50_id": "UniRef50_Q9PQP0", "cog_id": "COG0563", "cog_category": "F", "go_ids": [ "GO:0005737", "GO:0043231", "GO:0004017", "GO:0005524", "GO:0008270", "GO:0044209" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQP0", "EC:2.7.4.3", "UniRef:UniRef50_Q9PQP0", "COG:COG0563", "COG:F", "GO:0005737", "GO:0043231", "GO:0004017", "GO:0005524", "GO:0008270", "GO:0044209" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQP0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQP0" ], "product": "Adenylate kinase" } }, "id": "NKIECMJPJD_1100", "locus": "GCA000828735_01097" }, { "type": "cds", "sequence": "contig_1", "start": 639481, "stop": 639603, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCTCATTTGGTTGCTTTAGTAGCTAAAACAATCAATCATGGAATTCCTGTAATTCAACCATCAACAATTTTTGGAGGAACTTCAATTATTATTATGGTTACAGCAACGCTTGAATTATGA", "aa": "MPHLVALVAKTINHGIPVIQPSTIFGGTSIIIMVTATLEL", "aa_hexdigest": "dd5d98eb190df900437e894e3032b5ed", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4198.0427, "isoelectric_point": 6.693041419982911 }, "id": "NKIECMJPJD_1101", "locus": "GCA000828735_01098" }, { "type": "cds", "sequence": "contig_1", "start": 639637, "stop": 639738, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTGAAGATATTAAAAAAGCCGGTCGTTTTATTCCAGGTGTTCAAGTTGGAATGGATACTGAAAAACATATTACAAAAGTAATTTATCGCGTGAATTGA", "aa": "MAEDIKKAGRFIPGVQVGMDTEKHITKVIYRVN", "aa_hexdigest": "a88124ab4f4ab24139071ccfb96c6921", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3745.375900000001, "isoelectric_point": 9.397636985778806 }, "id": "NKIECMJPJD_1102", "locus": "GCA000828735_01099" }, { "type": "cds", "sequence": "contig_1", "start": 639817, "stop": 640779, "strand": "-", "frame": 3, "gene": "secY", "product": "preprotein translocase subunit SecY", "db_xrefs": [ "COG:COG0201", "COG:U", "GO:0015031", "GO:0016020", "GO:0043952", "SO:0001217", "UniRef:UniRef50_Q9PQP1", "UniRef:UniRef90_Q9PQP1" ], "nt": "ATGACTAACAAACAGAAGAAAAAAAATGCTTTTAGACAGTTGTTAATGATCTTTAAAAATAAAAAAGTTTTAGTTGCATTAATTGTAACTTTAAGTATATTAATCTTGTTTAGAATTGGATCTGTTATCCCAATGCCATACATTAAGTTAAATGGTAATTTTGGCAATCAAGGTTCATTCTTTTCAATTATTAACTTATTAGGCGGGGGAGGGCTATCACAATTTTCACTCTTCGCAATTGGGATAGGACCTTATATTACCGCTCAAATTATTATGCAACTTTTATCAAGTGAGCTTGTGCCACCACTCGCCAAATTATCCAAATCAGGTGAGCGTGGTCGTAAAAAAATTGAAGTGATTACAAGAATCATTACCTTGCCTCTTGCAGTGATGCAAGCTGTCATTATTATTAATTTAATGACAAGAGCAAATGGATTTATTTCAATTGTTTCAAATGCACCATTTGCTATTGGTTCACCATTATTTTATGTAACATACATTTTTTTAATGGTTGGTGGCACTTATATTTCGTTATTTTTAGCTGATCTAATTTCGAAAAAAGGTGTTGGTAATGGAATTACATTATTAATTCTTACAGGAATTGTTGCTTCATTATTTAATCACTTTATTGCAATTTTTTCAAATTTAGGTAGTTTAACAAGCAGTAAAGTTTCACAAATTATCGGTTTCATTTTATACATTTTATTCTATATTATGATCTTAATTGGTGTTGTTTTTGTTAATAATTCAACAAGAAAAATTCCAGTTCAACAAACCGGTCAAGCTCTAATTTTAGATCATGAGAAACTACCATTTCTTCCTATTAAAATTATGACAGCAGGAGTAATGCCAGTGATCTTTGCATCATCTGTTTTAGCCATTCCTGCACAAGTTGCTGAATTTTTAGATAAACAAAGCATGGGTTATTATGTGATTCATAATTACTTCATTGTTGATTCTTGA", "aa": "MTNKQKKKNAFRQLLMIFKNKKVLVALIVTLSILILFRIGSVIPMPYIKLNGNFGNQGSFFSIINLLGGGGLSQFSLFAIGIGPYITAQIIMQLLSSELVPPLAKLSKSGERGRKKIEVITRIITLPLAVMQAVIIINLMTRANGFISIVSNAPFAIGSPLFYVTYIFLMVGGTYISLFLADLISKKGVGNGITLLILTGIVASLFNHFIAIFSNLGSLTSSKVSQIIGFILYILFYIMILIGVVFVNNSTRKIPVQQTGQALILDHEKLPFLPIKIMTAGVMPVIFASSVLAIPAQVAEFLDKQSMGYYVIHNYFIVDS", "aa_hexdigest": "f1c20715df3394c417a98113be9a52b1", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "secY" ], "truncated": "5-prime", "pseudogene": { "start": 639514, "stop": 640779, "observations": { "mutation": [ 639639, 639819 ], "stop": [ 639639, 639819 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQP1", "query_cov": 1.0, "subject_cov": 0.6794055201698513, "identity": 0.9940000000000001, "score": 578.0, "evalue": 3.27e-205, "gene_start": 1, "gene_end": 320, "reference_start": 1, "reference_end": 320, "reference_sequence": "MTNKQKKKNAFRQLLMIFKNKKVLVALIVTLSILILFRIGSVIPMPYIKLNGNFGNQGSFFSIINLLGGGGLSQFSLFAIGIGPYITAQIIMQLLSSELVPPLAKLSKSGERGRKKIEVITRIITLPLAVMQAVIIINLMTRANGFISIVPNAPFAIGSPLFYVTYIFLMVGGTYISLFLADLISKKGVGNGITLLILTGIVASLFNHFIAIFSNLGSLTSSKVSQIIGFILYILFYIMILIGVVFVNNSTRKIPIQQTGQALILDHEKLPFLPIKIMTAGVMPVIFASSVLAIPAQVAEFLDKQSMGYYVIHNYFIVDSWTGLAIYVVLILLFTFFFSYVQLNPPKMAEDIKKAGRFIPGVQVGMDTEKHITKVIYRVNWIGAPILAFLACLPHLVALVAKTINHGIPVIQPSTIFGGTSIIIMVTATLELWNAIKSTSTSTSYAYQRKELETAITISVESDKSSKSQIW" }, "paralog": false, "identity": 0.9904988123515439, "score": 797.0, "evalue": 4.01e-294, "description": "Internal stop codon at 639639, 639819. Nonsense mutation around 639639, 639819.", "psc": { "uniref90_id": "UniRef90_Q9PQP1", "gene": "secY", "product": "preprotein translocase subunit SecY", "uniref50_id": "UniRef50_Q9PQP1", "cog_id": "COG0201", "cog_category": "U", "go_ids": [ "GO:0015031", "GO:0016020", "GO:0043952" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQP1", "UniRef:UniRef50_Q9PQP1", "COG:COG0201", "COG:U", "GO:0015031", "GO:0016020", "GO:0043952" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQP1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQP1" ], "product": "Protein translocase subunit SecY" } }, "id": "NKIECMJPJD_1103", "locus": "GCA000828735_01100" }, { "type": "cds", "sequence": "contig_1", "start": 640832, "stop": 641278, "strand": "-", "frame": 2, "gene": "rplO", "product": "50S ribosomal protein L15", "db_xrefs": [ "COG:COG0200", "COG:J", "GO:0003735", "GO:0006412", "GO:0015934", "GO:0019843", "GO:0022625", "KEGG:K02876", "RefSeq:WP_006688932.1", "SO:0001217", "UniParc:UPI00000C1BE9", "UniRef:UniRef100_Q9PQP2", "UniRef:UniRef50_Q9PQP2", "UniRef:UniRef90_Q9PQP2" ], "nt": "ATGCAATTACATAATTTAGAATATAAAAAAGGTTCAAGAAACCACAAAGAAAAACGTGTTGGTCGTGGTCATGGTTCTGGTTTAGGAAAAACTTCTGGGCGTGGACAAGATGGACAAAAAGCACGTAAATCAGGAATGGTACGTTTAGCTTTTGAAGGAGGACAAACACCACTTTATCGTCGTGTGCCAAAAGTTGGTTTTAACAACGATCGTTTTGCAAATAAATATAACGTTGTAACTTTAATTTCATTAGTAAAATATGAAACAAAAGAATTAACAGTTGAATTTATGTATGCAAATAAAATTGCTAAAAATGAAGATTTACCAATTAAGGTTATAGGTAATGCAGTTTTACCAAGTGGCACAGTAGTAAGTGCTCACAAATTTAGTAAAGGCGCATTGGAATCAATTAGTAATTCAAAAGCAAAAGCACAAATTTTAGAGTAA", "aa": "MQLHNLEYKKGSRNHKEKRVGRGHGSGLGKTSGRGQDGQKARKSGMVRLAFEGGQTPLYRRVPKVGFNNDRFANKYNVVTLISLVKYETKELTVEFMYANKIAKNEDLPIKVIGNAVLPSGTVVSAHKFSKGALESISNSKAKAQILE", "aa_hexdigest": "098c8bbdc49d02b044f81e6b4bce33b3", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI00000C1BE9", "ncbi_nrp_id": "WP_006688932.1", "uniref100_id": "UniRef100_Q9PQP2", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1BE9", "RefSeq:WP_006688932.1", "UniRef:UniRef100_Q9PQP2" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQP2", "uniref90_id": "UniRef90_Q9PQP2" }, "psc": { "uniref90_id": "UniRef90_Q9PQP2", "gene": "rplO", "product": "50S ribosomal protein L15", "uniref50_id": "UniRef50_Q9PQP2", "cog_id": "COG0200", "cog_category": "J", "kegg_orthology_id": "K02876", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015934", "GO:0019843", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQP2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQP2" ], "product": "Large ribosomal subunit protein uL15" }, "genes": [ "rplO" ], "id": "NKIECMJPJD_1104", "locus": "GCA000828735_01101" }, { "type": "cds", "sequence": "contig_1", "start": 641295, "stop": 641906, "strand": "-", "frame": 1, "gene": "rpsE", "product": "30S ribosomal protein S5", "db_xrefs": [ "COG:COG0098", "COG:J", "GO:0003735", "GO:0005737", "GO:0006412", "GO:0015935", "GO:0019843", "KEGG:K02988", "RefSeq:WP_006688963.1", "SO:0001217", "UniParc:UPI0000135080", "UniRef:UniRef100_Q9PQP3", "UniRef:UniRef50_B5ZB57", "UniRef:UniRef90_B5ZB57" ], "nt": "ATGGAAAACAATGTTAAAAAAGAAACTATTGTAGATAGTGAAAAAGTTGAAAAGCAACAACTAGTAACTGCACCAGTAGTTAATAAAAAAGAAAATACAGAACCAAAAGCAAAAACATTTAAACGTGAAACAACAACATCAAATTTTGAAGAACGTGTAGTGAAAATTAAACGAATTTCAAAAACAACAAAAGGTGGACGTATGATGCGTTTTAGTGCTTTAGTTGTTATTGGTGATAAAAATGGAACTGTTGGTTTTGGTATGGGTAAATCAATTGAAGTTCCAGATGCAATCAAAAAAGCGATTAAAAACGCAAACAATAATTTAATTAAAGTTAAGCAAACAAAAAAAGGTTCAATTTACCATGATGTAAATGGTCGTCATGGAGCTGCTAAAGTAATGTTACTACCTGCTCCTGAAGGTACTGGAATTATTGCTGGTGGACCTGTACGTGCTGTGGTTGAATTAGCTGGATTTACTGATATTTATACAAAATCACGTGGTGCTAATGCGCCAATGAATGTTATTCGTGCCACAATCAATGGGTTATTACAACAATTAACACCAAAAGAAATTGCACGTTTACGTGACAAAAGTTTAAGAGAAATATAG", "aa": "MENNVKKETIVDSEKVEKQQLVTAPVVNKKENTEPKAKTFKRETTTSNFEERVVKIKRISKTTKGGRMMRFSALVVIGDKNGTVGFGMGKSIEVPDAIKKAIKNANNNLIKVKQTKKGSIYHDVNGRHGAAKVMLLPAPEGTGIIAGGPVRAVVELAGFTDIYTKSRGANAPMNVIRATINGLLQQLTPKEIARLRDKSLREI", "aa_hexdigest": "03011c2430be4e3a61b66f220ae969b9", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0000135080", "ncbi_nrp_id": "WP_006688963.1", "uniref100_id": "UniRef100_Q9PQP3", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000135080", "RefSeq:WP_006688963.1", "UniRef:UniRef100_Q9PQP3" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQP3", "uniref90_id": "UniRef90_B5ZB57" }, "psc": { "uniref90_id": "UniRef90_B5ZB57", "gene": "rpsE", "product": "30S ribosomal protein S5", "uniref50_id": "UniRef50_B5ZB57", "cog_id": "COG0098", "cog_category": "J", "kegg_orthology_id": "K02988", "go_ids": [ "GO:0003735", "GO:0005737", "GO:0006412", "GO:0015935", "GO:0019843" ] }, "pscc": { "uniref50_id": "UniRef50_B5ZB57", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B5ZB57" ], "product": "Small ribosomal subunit protein uS5" }, "genes": [ "rpsE" ], "id": "NKIECMJPJD_1105", "locus": "GCA000828735_01102" }, { "type": "cds", "sequence": "contig_1", "start": 642155, "stop": 642289, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAGAATTAATTTTAGTCGTGCTAAACAACGTGCACTTCGTGCTAAACGTCTTCATGTTAAAATTCGTAACCTTCAATTAGCTGCAAACAAACCTGTTTTAGTTATTACAAAAACAAATGCACACATTTGA", "aa": "MKRINFSRAKQRALRAKRLHVKIRNLQLAANKPVLVITKTNAHI", "aa_hexdigest": "bcc7f38189c474b2819c8b763ad955f1", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5118.1596, "isoelectric_point": 11.999967765808105 }, "id": "NKIECMJPJD_1106", "locus": "GCA000828735_01103" }, { "type": "cds", "sequence": "contig_1", "start": 642605, "stop": 642856, "strand": "-", "frame": 2, "gene": "rplF", "product": "50S ribosomal protein L6", "db_xrefs": [ "COG:COG0097", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "SO:0001217", "UniRef:UniRef50_P47412", "UniRef:UniRef90_Q9PQP5" ], "nt": "ATGTCAAGAATTGGAAATCGTAAATTAACTATTCCAGCTAATGTAAATGTTAGTGTTGAATCTGGTAAAGTACATATTGTTAGTCAAACTGCAAAATTAAGTGTTGATTTTCCAGTAAATCTAATTAGTGTAGATGTTGTTGATAATACTGTTAAAGTTAGTCGTGCAAACGATGAAAAACAAACAAAAATGTTTCATGGAACAGTAAATGCAAATATTGCTAATGCTTTAGTTGGTGTAACTACTGGTTGA", "aa": "MSRIGNRKLTIPANVNVSVESGKVHIVSQTAKLSVDFPVNLISVDVVDNTVKVSRANDEKQTKMFHGTVNANIANALVGVTTG", "aa_hexdigest": "53fa92a1a5d0ab3be723ff27cc499450", "start_type": "ATG", "rbs_motif": null, "genes": [ "rplF" ], "truncated": "5-prime", "pseudogene": { "start": 642308, "stop": 642856, "observations": { "mutation": [ 642607 ], "stop": [ 642607 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQP5", "query_cov": 1.0, "subject_cov": 0.45604395604395603, "identity": 0.988, "score": 157.0, "evalue": 1.48e-47, "gene_start": 1, "gene_end": 83, "reference_start": 1, "reference_end": 83, "reference_sequence": "MSRIGNRKLTIPANVNVSVESGKVHIVSQTAKLSVDFPVNLISVDVVDNTIKVSRANDEKQTKMFHGTVNANIANALVGVTTGWKKELEVKGVGFRAKVEGSKLNLGLGFSHPLLIQIPTGLKIETPSATEISISGSDKAAVGAFAAVVRAYRKPEPYKGKGVMYKGERIVRKAGKTADKKK" }, "paralog": false, "identity": 0.9725274725274725, "score": 345.0, "evalue": 3.86e-124, "description": "Internal stop codon at 642607. Nonsense mutation around 642607.", "psc": { "uniref90_id": "UniRef90_Q9PQP5", "gene": "rplF", "product": "50S ribosomal protein L6", "uniref50_id": "UniRef50_P47412", "cog_id": "COG0097", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQP5", "UniRef:UniRef50_P47412", "COG:COG0097", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843" ] }, "pscc": { "uniref50_id": "UniRef50_P47412", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47412" ], "product": "Large ribosomal subunit protein uL6" } }, "id": "NKIECMJPJD_1107", "locus": "GCA000828735_01104" }, { "type": "cds", "sequence": "contig_1", "start": 642868, "stop": 643263, "strand": "-", "frame": 3, "gene": "rpsH", "product": "30S ribosomal protein S8", "db_xrefs": [ "COG:COG0096", "COG:J", "GO:0003735", "GO:0005737", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904", "SO:0001217", "UniRef:UniRef50_Q9PQP6", "UniRef:UniRef90_Q9PQP6" ], "nt": "ATGTATTTAGATCCAATTGCTGAATTAATTACAAAAATTAATAATGGTCGTAAGGCCCATAAAGCTGAAGTTAGTTTTGCAACTTCAAAGCTTAAAACAGCGATTTTAGAATTATTGGTTAAAGAAGGATATATCAAATCTTATGATATTCGTCCAACTGAAAACAATAAATCTGAAACAGTTGTTAAACTAAAATACAAAAACCAAACAACATCTTCTATCAATGGTTTTAAACAAATTTCAAAACCAGGATTAAGAATTTATTCAACACACTTAAATTTACCAAAAGTATTAAATGGTTTAGGAATTGCTATCATCACAACTTCAAAAGGTGTTATGTCAGATAAACAAGCAAGAAAGGAAAATGTAGGCGGGGAAGTAATCGCTTACGTTTGA", "aa": "MYLDPIAELITKINNGRKAHKAEVSFATSKLKTAILELLVKEGYIKSYDIRPTENNKSETVVKLKYKNQTTSSINGFKQISKPGLRIYSTHLNLPKVLNGLGIAIITTSKGVMSDKQARKENVGGEVIAYV", "aa_hexdigest": "1241dfa8efc1e17adcfe51df4943df54", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_Q9PQP6", "query_cov": 1.0, "subject_cov": 0.9924242424242424, "identity": 1.0, "score": 252.0, "evalue": 7.15e-85, "valid": true, "gene": "rpsH", "product": "30S ribosomal protein S8", "uniref50_id": "UniRef50_Q9PQP6", "cog_id": "COG0096", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005737", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQP6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQP6" ], "product": "Small ribosomal subunit protein uS8" }, "genes": [ "rpsH" ], "id": "NKIECMJPJD_1108", "locus": "GCA000828735_01105" }, { "type": "cds", "sequence": "contig_1", "start": 643277, "stop": 643459, "strand": "-", "frame": 2, "gene": "rpsZ", "product": "type Z 30S ribosomal protein S14", "db_xrefs": [ "COG:COG0199", "COG:J", "GO:0003735", "GO:0005737", "GO:0005840", "GO:0006412", "GO:0008270", "GO:0015935", "GO:0019843", "KEGG:K02954", "SO:0001217", "UniRef:UniRef50_Q9PQP7", "UniRef:UniRef90_Q9PQP7" ], "nt": "ATGGCGAAAAAATCATTAATTGCTAAGCAAAAAAAACATCAAAAATTTGCAGTTCGTGAATACACTCGTTGCGTAAGATGTGGTCGTCCACATGCTGTTAATCGTAAATTTGGTGTATGTCGTCTTTGCTTTAGAGATTTAGCTTACGCTGGTGCAATTCCAGGAATTAAGAAAGCATCTTGA", "aa": "MAKKSLIAKQKKHQKFAVREYTRCVRCGRPHAVNRKFGVCRLCFRDLAYAGAIPGIKKAS", "aa_hexdigest": "1c4225f1605cc04d44260c747731166c", "start_type": "ATG", "rbs_motif": "AGGAG/GGAGG", "psc": { "uniref90_id": "UniRef90_Q9PQP7", "query_cov": 1.0, "subject_cov": 0.9836065573770492, "identity": 1.0, "score": 124.0, "evalue": 2.71e-36, "valid": true, "gene": "rpsZ", "product": "type Z 30S ribosomal protein S14", "uniref50_id": "UniRef50_Q9PQP7", "cog_id": "COG0199", "cog_category": "J", "kegg_orthology_id": "K02954", "go_ids": [ "GO:0003735", "GO:0005737", "GO:0005840", "GO:0006412", "GO:0008270", "GO:0015935", "GO:0019843" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQP7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQP7" ], "product": "Small ribosomal subunit protein uS14" }, "genes": [ "rpsZ" ], "id": "NKIECMJPJD_1109", "locus": "GCA000828735_01106" }, { "type": "cds", "sequence": "contig_1", "start": 643463, "stop": 643573, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCGTGGTTTTGATGTAACATTTGTTACAACTGCAAAAACAGCCCAAGAAGCTAAAGCATTACTAGTGGGACTAGGTGCTCCATTTCAAAAAGTAAGAGGAGACAAATAA", "aa": "MRGFDVTFVTTAKTAQEAKALLVGLGAPFQKVRGDK", "aa_hexdigest": "0933bb9427471704bac5bf4f1b649386", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3852.462800000001, "isoelectric_point": 9.991971015930176 }, "id": "NKIECMJPJD_1110", "locus": "GCA000828735_01107" }, { "type": "cds", "sequence": "contig_1", "start": 643727, "stop": 644017, "strand": "-", "frame": 2, "gene": "rplE", "product": "50S ribosomal protein L5", "db_xrefs": [ "COG:COG0094", "COG:J", "GO:0000049", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904", "KEGG:K02931", "SO:0001217", "UniRef:UniRef50_Q9PQP8", "UniRef:UniRef90_Q9PQP8" ], "nt": "ATGGCATTCTTAAAAGATTTATATAAAAATAAAGTTGCTAAAGATTTGCAAAAAGAATTTGCTTATTCTTCTGTAATGCAAATTCCTAAAATTGAAAAAGTAGTTATTAACGCAGGAATTGGAAATGCTGTTGCTGATAAAAAACATTTAGAAGCAGCTATTTCAGAATTAACTTTGATTACAGGACAAAGACCTGTTGAAACAAAAGCTAAAAAATCAATTGCAACATTTAAATTACGTGCTGGTCAAAGTATTGGTGCTAAAGTTACATTACGTGGCGATCGTATGTGA", "aa": "MAFLKDLYKNKVAKDLQKEFAYSSVMQIPKIEKVVINAGIGNAVADKKHLEAAISELTLITGQRPVETKAKKSIATFKLRAGQSIGAKVTLRGDRM", "aa_hexdigest": "ce18672de5e0948748c2c5e551fad492", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_UPI001BFFEF50", "query_cov": 1.0, "subject_cov": 0.8648648648648649, "identity": 0.563, "score": 111.0, "evalue": 7.54e-30, "valid": false, "product": "50S ribosomal protein L5", "uniref50_id": "UniRef50_A0A2X1PYD2", "kegg_orthology_id": "K02931" }, "genes": [ "rplE" ], "truncated": "5-prime", "pseudogene": { "start": 643463, "stop": 644017, "observations": { "mutation": [ 643729 ], "stop": [ 643729 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQP8", "query_cov": 1.0, "subject_cov": 0.5217391304347826, "identity": 1.0, "score": 181.0, "evalue": 1.44e-56, "gene_start": 1, "gene_end": 96, "reference_start": 1, "reference_end": 96, "reference_sequence": "MAFLKDLYKNKVAKDLQKEFAYSSVMQIPKIEKVVINAGIGNAVADKKHLEAAISELTLITGQRPVETKAKKSIATFKLRAGQSIGAKVTLRGDRMWAFIETLFNIALPRVRDFKGISNNSFDNQGNYTLGIKEQIIFPQVVYDDVKSVRGFDVTFVTTAKTAQEAKALLVGLGAPFQKVRGDK" }, "paralog": false, "identity": 0.9945652173913043, "score": 352.0, "evalue": 1.75e-126, "description": "Internal stop codon at 643729. Nonsense mutation around 643729.", "psc": { "uniref90_id": "UniRef90_Q9PQP8", "gene": "rplE", "product": "50S ribosomal protein L5", "uniref50_id": "UniRef50_Q9PQP8", "cog_id": "COG0094", "cog_category": "J", "kegg_orthology_id": "K02931", "go_ids": [ "GO:0000049", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQP8", "UniRef:UniRef50_Q9PQP8", "COG:COG0094", "COG:J", "KEGG:K02931", "GO:0000049", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQP8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQP8" ], "product": "Large ribosomal subunit protein uL5" } }, "id": "NKIECMJPJD_1111", "locus": "GCA000828735_01108" }, { "type": "cds", "sequence": "contig_1", "start": 644034, "stop": 644366, "strand": "-", "frame": 1, "gene": "rplX", "product": "50S ribosomal protein L24", "db_xrefs": [ "COG:COG0198", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904", "RefSeq:WP_006688875.1", "SO:0001217", "UniParc:UPI00000C1BE5", "UniRef:UniRef100_Q9PQP9", "UniRef:UniRef50_O52343", "UniRef:UniRef90_Q9PQP9" ], "nt": "ATGAACAGAATTAAAAAAGGCGATACAGTTGTTGTTATTTCAGGTAAAAACAAAAATAAATCTGGCGTAGTTATTCAAGTTAACCCTAAAGAACAAACAGCACTTGTTGAAGGCGTTAATAAAATTAAACGTCATCAAAAGAAAGATCAAACGCATGAACAATCTGGAATTATTGAAAAAGAAGCTCCAATTCGTTTATGTAAATTAGCATTAGTTGATCCTAAAGGAAAAGATAAAGGTAAAGCAACTAAAGTTAAATATTTACTTAAAGATAACAAAAAAGTTCGTGTAGCACGTAAAAGTGGTTCAGAACTTGATGTAAATAAAAAATAA", "aa": "MNRIKKGDTVVVISGKNKNKSGVVIQVNPKEQTALVEGVNKIKRHQKKDQTHEQSGIIEKEAPIRLCKLALVDPKGKDKGKATKVKYLLKDNKKVRVARKSGSELDVNKK", "aa_hexdigest": "896ddee55bf86af351d6a914358a9e59", "start_type": "ATG", "rbs_motif": "GGAGG", "ups": { "uniparc_id": "UPI00000C1BE5", "ncbi_nrp_id": "WP_006688875.1", "uniref100_id": "UniRef100_Q9PQP9", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1BE5", "RefSeq:WP_006688875.1", "UniRef:UniRef100_Q9PQP9" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQP9", "uniref90_id": "UniRef90_Q9PQP9" }, "psc": { "uniref90_id": "UniRef90_Q9PQP9", "gene": "rplX", "product": "50S ribosomal protein L24", "uniref50_id": "UniRef50_O52343", "cog_id": "COG0198", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_O52343", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_O52343" ], "product": "Large ribosomal subunit protein uL24" }, "genes": [ "rplX" ], "id": "NKIECMJPJD_1112", "locus": "GCA000828735_01109" }, { "type": "cds", "sequence": "contig_1", "start": 644381, "stop": 644749, "strand": "-", "frame": 2, "gene": "rplN", "product": "50S ribosomal protein L14", "db_xrefs": [ "COG:COG0093", "COG:J", "GO:0003735", "GO:0006412", "GO:0015934", "GO:0022625", "GO:0070180", "KEGG:K02874", "RefSeq:WP_004026223.1", "SO:0001217", "UniParc:UPI00000C1BE4", "UniRef:UniRef100_Q9PQQ0", "UniRef:UniRef50_A5FMZ0", "UniRef:UniRef90_Q9PQQ0" ], "nt": "ATGATTCAACATATGACAAGACTAAAAGTTGCAGATAACACAGGTGCTAAAGAAGTTGGAGTAATCAAAGTGCTTGGTGGCTCTAAAAAACGTTATGCATCAGTGGGCGATATTGTAGTTGTCTCAGTTAAAAAAGCAACACCTGCTGGTTTAATTGCTAAAGGACAAATGGCCAAAGCTGTAATTGTTAGAACAAAAAAATCAATTCGTCGTGAGTCTGGTTTATTAATTAGATTTGACGAAAACGCATGTGTACTTATTAAAGAGGATAAGACACCAAGAGGATCACGTATTTTTGGTCCTGTAGCAAGAGAAATTCGTGATCGTGGATACACAAAAATTGCTTCTCTTGCTCCTGAAGTATTATAG", "aa": "MIQHMTRLKVADNTGAKEVGVIKVLGGSKKRYASVGDIVVVSVKKATPAGLIAKGQMAKAVIVRTKKSIRRESGLLIRFDENACVLIKEDKTPRGSRIFGPVAREIRDRGYTKIASLAPEVL", "aa_hexdigest": "2e62b6cc554c5edb854b3d4f2179b4f3", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI00000C1BE4", "ncbi_nrp_id": "WP_004026223.1", "uniref100_id": "UniRef100_Q9PQQ0", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1BE4", "RefSeq:WP_004026223.1", "UniRef:UniRef100_Q9PQQ0" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQQ0", "uniref90_id": "UniRef90_Q9PQQ0" }, "psc": { "uniref90_id": "UniRef90_Q9PQQ0", "gene": "rplN", "product": "50S ribosomal protein L14", "uniref50_id": "UniRef50_A5FMZ0", "cog_id": "COG0093", "cog_category": "J", "kegg_orthology_id": "K02874", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015934", "GO:0022625", "GO:0070180" ] }, "pscc": { "uniref50_id": "UniRef50_A5FMZ0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A5FMZ0" ], "product": "Large ribosomal subunit protein uL14" }, "genes": [ "rplN" ], "id": "NKIECMJPJD_1113", "locus": "GCA000828735_01110" }, { "type": "cds", "sequence": "contig_1", "start": 644802, "stop": 645023, "strand": "-", "frame": 1, "gene": "rpsQ", "product": "30S ribosomal protein S17", "db_xrefs": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022627", "SO:0001217", "UniRef:UniRef50_Q9PQQ1", "UniRef:UniRef90_Q9PQQ1" ], "nt": "ATGGAAAGAAGCCGTCGTAAAGTTTTAGAAGGGTTAGTAGTTTCAGATAAAATGCAAAAAACTGTTGTTGTTAGTGTTGAAACTAAAAGCAAACACCCAATATATAGAAAACTTGTAATTAGTCATAAAAAATACCACGCACACAATGATAATGATGATGCCAAAGTTGGTGACCTTGTTGAAATCACTGAAACTCGTCCATTAAGTGCAACAAAAAATTGA", "aa": "MERSRRKVLEGLVVSDKMQKTVVVSVETKSKHPIYRKLVISHKKYHAHNDNDDAKVGDLVEITETRPLSATKN", "aa_hexdigest": "8960cdd2dfad6911a387dc2958864098", "start_type": "ATG", "rbs_motif": "GGAGG", "psc": { "uniref90_id": "UniRef90_Q9PQQ1", "query_cov": 1.0, "subject_cov": 0.8690476190476191, "identity": 1.0, "score": 142.0, "evalue": 8.53e-43, "valid": true, "gene": "rpsQ", "product": "30S ribosomal protein S17", "uniref50_id": "UniRef50_Q9PQQ1", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022627" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQQ1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQQ1" ], "product": "Small ribosomal subunit protein uS17" }, "genes": [ "rpsQ" ], "id": "NKIECMJPJD_1114", "locus": "GCA000828735_01111" }, { "type": "cds", "sequence": "contig_1", "start": 645023, "stop": 645250, "strand": "-", "frame": 2, "gene": "rpmC", "product": "50S ribosomal protein L29", "db_xrefs": [ "COG:COG0255", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625", "RefSeq:WP_004026203.1", "SO:0001217", "UniParc:UPI0000133E9C", "UniRef:UniRef100_Q9PQQ2", "UniRef:UniRef50_Q9PQQ2", "UniRef:UniRef90_Q9PQQ2" ], "nt": "ATGAGTAGTATTGCACAAGATCTTCGTAAAAAAGATAGCTTAGAACTTGAAAAAATTGTTATTGAGCTTAAAGCCAAATTATTAGAACTTCGTTTTGCAGCTGCGAATGGGGAAGCAGAAAAACTTCATACAGCCAAAGAAATTCGTAAAACAATTGCACGTGCATTAACAATTTTAAATGAACGTGAATTAGCAGAAAAATTAAACAACAAGGAGGCTAATAAATAA", "aa": "MSSIAQDLRKKDSLELEKIVIELKAKLLELRFAAANGEAEKLHTAKEIRKTIARALTILNERELAEKLNNKEANK", "aa_hexdigest": "a9d5b64f250cc5d0daea5f598b680b0d", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI0000133E9C", "ncbi_nrp_id": "WP_004026203.1", "uniref100_id": "UniRef100_Q9PQQ2", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000133E9C", "RefSeq:WP_004026203.1", "UniRef:UniRef100_Q9PQQ2" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQQ2", "uniref90_id": "UniRef90_Q9PQQ2" }, "psc": { "uniref90_id": "UniRef90_Q9PQQ2", "gene": "rpmC", "product": "50S ribosomal protein L29", "uniref50_id": "UniRef50_Q9PQQ2", "cog_id": "COG0255", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQQ2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQQ2" ], "product": "Large ribosomal subunit protein uL29" }, "genes": [ "rpmC" ], "id": "NKIECMJPJD_1115", "locus": "GCA000828735_01112" }, { "type": "cds", "sequence": "contig_1", "start": 645250, "stop": 645360, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTGAAATTGCTAATGTTTCAGAAGAATTAATGTGTGAAGCTTTACGTGCTGCTGGAAATAAGTTACCAATTAAAGTAAAAATTGTTAAGAAGGGAGAAGCTAACTAA", "aa": "MFEIANVSEELMCEALRAAGNKLPIKVKIVKKGEAN", "aa_hexdigest": "13c37d9a40916fbdc6e9b3b7a962e545", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3945.672200000001, "isoelectric_point": 7.90119686126709 }, "id": "NKIECMJPJD_1116", "locus": "GCA000828735_01113" }, { "type": "cds", "sequence": "contig_1", "start": 645544, "stop": 645666, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00252.24" ], "nt": "ATGTTACAACCAAAAAGAACAAAATTTCGTAAGCCTCATAAAGTAAGCTACGAAGGTAAAGCTAAAGGAAATAAACAAGTTGATTTTGGTGAATTTGGATTAATGGCACTAGAAGGTGCATGA", "aa": "MLQPKRTKFRKPHKVSYEGKAKGNKQVDFGEFGLMALEGA", "aa_hexdigest": "29b04c372ff18cbedce23b5c04d62a02", "start_type": "ATG", "rbs_motif": "AGGAG/GGAGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00252.24", "name": "Ribosomal_L16", "length": 37, "aa_cov": 0.9, "hmm_cov": 0.972972972972973, "evalue": 3.090380797223197e-08, "score": 29.23223876953125, "start": 4, "stop": 39 } ], "seq_stats": { "molecular_weight": 4523.2456999999995, "isoelectric_point": 9.9959680557251 }, "id": "NKIECMJPJD_1117", "locus": "GCA000828735_01114" }, { "type": "cds", "sequence": "contig_1", "start": 645843, "stop": 646100, "strand": "-", "frame": 1, "gene": null, "product": "30S ribosomal protein S3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7V6MUE3" ], "nt": "ATGGCACGTGAAATTGCCGACGCAATTGAAAATAGAGTTTCATTTAGAATTGCTCAAAAAATGGTAATTAAAAAAGTTCTAAAAGCTGGTGCTAGAGGTATTAAAACACATGTTTCAGGTCGTTTAGGTGGCGTGGAAATGGCACGTGAAGAAGGATATACACAAGGTGTAATGACCTTACATACATTACGTGCAGATATTGATTATTCAATGCAAGAAGCACATACAACTTATGGAATTATTGGTGTTAAAGTTTGA", "aa": "MAREIADAIENRVSFRIAQKMVIKKVLKAGARGIKTHVSGRLGGVEMAREEGYTQGVMTLHTLRADIDYSMQEAHTTYGIIGVKV", "aa_hexdigest": "673c83f50747cd1fdec06c08f25e803d", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0031CD8184", "query_cov": 0.9529411764705882, "subject_cov": 0.84375, "identity": 0.63, "score": 102.0, "evalue": 1.42e-26, "valid": false, "gene": "rpsC", "product": "30S ribosomal protein S3", "uniref50_id": "UniRef50_A0A7V6MUE3", "kegg_orthology_id": "K02982", "go_ids": [ "GO:0003735", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_A0A7V6MUE3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7V6MUE3" ], "product": "30S ribosomal protein S3" }, "genes": [], "id": "NKIECMJPJD_1118", "locus": "GCA000828735_01115" }, { "type": "cds", "sequence": "contig_1", "start": 646648, "stop": 647409, "strand": "-", "frame": 3, "gene": "rplV", "product": "50S ribosomal protein L22", "db_xrefs": [ "COG:COG4933", "COG:K", "GO:0003735", "GO:0006412", "GO:0015934", "GO:0019843", "GO:0022625", "KEGG:K02890", "SO:0001217", "UniRef:UniRef50_Q9PQQ5", "UniRef:UniRef90_Q9PQQ5" ], "nt": "ATGACAAATAAAGTTATTCAAAGAAATATTCATATTTCGCACCGTAAAGCTTCTCTTGTAATTGATTTAGTTCGAAACAAGCCAGTACATGAAGCAATTCGAATTTTAAGTAATACACCAAAGAAGTTTGCTCCAATCGTTTTGAAATTACTTAACTCAGCAATTTCAAACGTTCAACATAACTCTAAAGATATGGATCCAAGTAAACTATATATTTACAAAATTGTAGCTAATCAAGGTCCAACAATGAAAAGAACACTACCACGTGCTAAGGGTTCTGCTGATCAATTATTTAAACGTACAACACATTTAGAAATTGTTTTATCTGATGATGTTAATGAACGTGAAAAAGAATTAGCAGCTATTAAAGCTAAAAAATCAAAAAAACCATTAGCAGTTGAACCGATTGCTAAAGTTGAAACTAAAAAAGTTGCTAAACCAAGCAAAGTAGAAATAAAACCAGTTGAAAAAGACGAAAACGTTGATCCAGAACTTTTAAAACGTGAACAACAAGTTTTAAAAGTTGTTGAAAAAACTGCTTCACAAAAAGAAGAAAAAACTACTGAAACAATTATGATTTCAACATCACCTAAGAATGCACAAGTTTTATTTGATGATTTAGAAAAAAATGTAATCTTTTATAAAACAACACCTATTAATAAAGTTTTACGTGTACTAGTTTATGTAACTAGCCCAACTAAAAAAGTTGTTGGTGAATTTGATTTAGAATCAGTTGAAATTGGTGCAATTTCATCAATTTGA", "aa": "MTNKVIQRNIHISHRKASLVIDLVRNKPVHEAIRILSNTPKKFAPIVLKLLNSAISNVQHNSKDMDPSKLYIYKIVANQGPTMKRTLPRAKGSADQLFKRTTHLEIVLSDDVNEREKELAAIKAKKSKKPLAVEPIAKVETKKVAKPSKVEIKPVEKDENVDPELLKREQQVLKVVEKTASQKEEKTTETIMISTSPKNAQVLFDDLEKNVIFYKTTPINKVLRVLVYVTSPTKKVVGEFDLESVEIGAISSI", "aa_hexdigest": "a6fd64c2b00a9398862185748982f2ef", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_Q9PQQ5", "query_cov": 1.0039525691699605, "subject_cov": 0.8141025641025641, "identity": 0.9690000000000001, "score": 448.0, "evalue": 1.49e-157, "valid": true, "gene": "rplV", "product": "50S ribosomal protein L22", "uniref50_id": "UniRef50_Q9PQQ5", "cog_id": "COG4933", "cog_category": "K", "kegg_orthology_id": "K02890", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015934", "GO:0019843", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQQ5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQQ5" ], "product": "Large ribosomal subunit protein uL22" }, "genes": [ "rplV" ], "id": "NKIECMJPJD_1119", "locus": "GCA000828735_01116" }, { "type": "cds", "sequence": "contig_1", "start": 647596, "stop": 647697, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCTAGAAGTTTAAAAAAAGGTGCTTACGCTGATCCTAGTTTATTAAAAAAAGTTGAAGCAGCTAATGCATCAGTTTCTAAAAAACCAATCAAAACATGA", "aa": "MSRSLKKGAYADPSLLKKVEAANASVSKKPIKT", "aa_hexdigest": "94b2bc5537068311c6202d944caf68bd", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3518.1343000000006, "isoelectric_point": 10.218255043029785 }, "id": "NKIECMJPJD_1120", "locus": "GCA000828735_01117" }, { "type": "cds", "sequence": "contig_1", "start": 647796, "stop": 648557, "strand": "-", "frame": 1, "gene": "rplB", "product": "50S ribosomal protein L2", "db_xrefs": [ "GO:0003723", "GO:0003735", "GO:0006412", "GO:0015934", "GO:0016740", "KEGG:K02886", "SO:0001217", "UniRef:UniRef50_Q9AMK8", "UniRef:UniRef90_A0A084F1R4" ], "nt": "ATGGCAGTTAAACGTATTAAAAACCATAGTAGTGGTAAAAGACAAACTGTTGTTGTTGATTACAAATCGATTTTAACAACAAGTAAACCAGAAAAATCATTGCTTGTTACTTTACCTAAAAAAGCAGGTAGAAACAATCAAGGGAAAATTACAATTAGACACCATGGTGGTGGTCATAAACGTAAATATCGTATTATTGACTTTAAAAGAAATAAAGATAATATTTATGGAACTATTAAATCAATTGAATACGATCCAAACAGAACATCATTTATTTCATTAGTTGTTTACGCTGATGGAGAAAAACGTTATATTATTGCTCCTAAAGGAATTAAAGTGGGCGATAAAATTATTTCAGGAAATGAAAACATTGATATTTTATTAGGTAATTCATTACCACTTGAATTTATTCCAGAAGGTACATTAGTTCACAATATTGAACTATCACCAAATGCTGGTGGTCAAATTACAAGAAGTGCTGGTGCTAGTGCACAAATTCTTGGTTTTGATGAAACTAAAAAATATATTTTAGTTAAATTAAACTCAGGCGAAGTTCGTAAATTTAGAAAAGAATGTCGTGCTACAATTGGAACTGTTTCAAATGATGAACACATTCTTGAAAATTTAGGTAAAGCTGGTAAATCACGTCATTTAGGGGTTCGTCCAACAGTTCGTGGTTCTGCAATGAACCCTAATGATCACCCACATGGGGGTGGTGAAGGTCGTTCACCAGTTGGTATGGATGCACCTCGTACACCTTGA", "aa": "MAVKRIKNHSSGKRQTVVVDYKSILTTSKPEKSLLVTLPKKAGRNNQGKITIRHHGGGHKRKYRIIDFKRNKDNIYGTIKSIEYDPNRTSFISLVVYADGEKRYIIAPKGIKVGDKIISGNENIDILLGNSLPLEFIPEGTLVHNIELSPNAGGQITRSAGASAQILGFDETKKYILVKLNSGEVRKFRKECRATIGTVSNDEHILENLGKAGKSRHLGVRPTVRGSAMNPNDHPHGGGEGRSPVGMDAPRTP", "aa_hexdigest": "1ded039aebb1719abd9f5df0a7913506", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_A0A084F1R4", "query_cov": 1.0, "subject_cov": 0.9068100358422939, "identity": 0.925, "score": 476.0, "evalue": 6.95e-169, "valid": true, "gene": "rplB", "product": "50S ribosomal protein L2", "uniref50_id": "UniRef50_Q9AMK8", "kegg_orthology_id": "K02886", "go_ids": [ "GO:0003723", "GO:0003735", "GO:0006412", "GO:0015934", "GO:0016740" ] }, "pscc": { "uniref50_id": "UniRef50_Q9AMK8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9AMK8" ], "product": "Large ribosomal subunit protein uL2" }, "genes": [ "rplB" ], "id": "NKIECMJPJD_1121", "locus": "GCA000828735_01118" }, { "type": "cds", "sequence": "contig_1", "start": 648611, "stop": 648928, "strand": "-", "frame": 2, "gene": "rplW", "product": "50S ribosomal protein L23", "db_xrefs": [ "COG:COG0089", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904", "RefSeq:WP_006689129.1", "SO:0001217", "UniParc:UPI00000C1BDF", "UniRef:UniRef100_Q9PQQ8", "UniRef:UniRef50_Q9PQQ8", "UniRef:UniRef90_Q9PQQ8" ], "nt": "ATGGAATTAACAAGAGTTATTTTACATCCATATACGACAGAAAAAACTTATTCTATTCGTAATAAAAGCGAGCATGAAACATTAACTTTTATTGTTGATAAAAATGCTAATAAATACCAAATTCGTGAAGCATTTATTGCGATTTTTGGTTTAAAACCACTTAAAATTCGTACTACAAATCGTCGTCCTGCAAAAATTCGTACTTCAACAGCTAGACCAGGATATACAAAAGCAAAAAAAATTGCTTATGTAGTAATGCCAGTAGGTGTTAAAGTAGCTGTAAGTAAAGAAGAAGTTGAAGCAGCAAACGCAAAGTAG", "aa": "MELTRVILHPYTTEKTYSIRNKSEHETLTFIVDKNANKYQIREAFIAIFGLKPLKIRTTNRRPAKIRTSTARPGYTKAKKIAYVVMPVGVKVAVSKEEVEAANAK", "aa_hexdigest": "fe0da67b84e0da48927f183a1a661b81", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "ups": { "uniparc_id": "UPI00000C1BDF", "ncbi_nrp_id": "WP_006689129.1", "uniref100_id": "UniRef100_Q9PQQ8", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1BDF", "RefSeq:WP_006689129.1", "UniRef:UniRef100_Q9PQQ8" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQQ8", "uniref90_id": "UniRef90_Q9PQQ8" }, "psc": { "uniref90_id": "UniRef90_Q9PQQ8", "gene": "rplW", "product": "50S ribosomal protein L23", "uniref50_id": "UniRef50_Q9PQQ8", "cog_id": "COG0089", "cog_category": "J", "go_ids": [ "GO:1990904", "GO:0005840", "GO:0019843", "GO:0003735", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQQ8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQQ8" ], "product": "Large ribosomal subunit protein uL23" }, "genes": [ "rplW" ], "id": "NKIECMJPJD_1122", "locus": "GCA000828735_01119" }, { "type": "cds", "sequence": "contig_1", "start": 649024, "stop": 649305, "strand": "-", "frame": 3, "gene": "rplD", "product": "50S ribosomal protein L4", "db_xrefs": [ "COG:COG0088", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022625", "GO:1990904", "SO:0001217", "UniRef:UniRef50_Q9PQQ9", "UniRef:UniRef90_Q9PQQ9" ], "nt": "GTGGTATTTGGTCCAAAACCAAATCGTAACTACAATTTAAAAGTTAATGCAAAAGTAAGACTATTAGCTTTCAAATCAGCATTAACTATTAAATTGAATGAAGGAAAAATGCTAGGTTTAGTAGCAAATAGCGATTTAGAAACACCTTCAACTAAAAAAATGGTAAATTTCATTAATAATGCTAACTTAGAAAATCAAAAAGTTTTATTAGTAATAGTCGACAATTTTAGTAATATTAAAAAATCAACAAACAATTTACAAAAAGTTACAACAAAATTATGA", "aa": "MVFGPKPNRNYNLKVNAKVRLLAFKSALTIKLNEGKMLGLVANSDLETPSTKKMVNFINNANLENQKVLLVIVDNFSNIKKSTNNLQKVTTKL", "aa_hexdigest": "5716e72a7e9a10ae23dcb952041a31a2", "start_type": "GTG", "rbs_motif": "AGxAGG/AGGxGG", "genes": [ "rplD" ], "truncated": "both", "pseudogene": { "start": 648931, "stop": 649566, "observations": { "mutation": [ 649026, 649320, 649374 ], "stop": [ 649026, 649320, 649374 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQQ9", "query_cov": 1.0, "subject_cov": 0.44075829383886256, "identity": 0.9890000000000001, "score": 174.0, "evalue": 1.12e-53, "gene_start": 1, "gene_end": 93, "reference_start": 88, "reference_end": 180, "reference_sequence": "MAKIKLLSIDGNFAKELEVTSDLFVEVPHKQAMFDSVLAENAAERQGTHSTLTKGEVRGGGKKPWRQKHTGKARTGSTRNPHWTGGGVVFGPKPNRNYNLKVNAKVRLLAFKSALTIKLNEGKMLGLVANSDLETPSTKKMVNFINNANLENQKVLLVIVDNFSNIKKSTNNLQKVTTKLWYQVSVRDLMHANVVVVAEEAFTNYARKVSK" }, "paralog": false, "identity": 0.985781990521327, "score": 402.0, "evalue": 6.61e-146, "description": "Internal stop codon at 649026, 649320, 649374. Nonsense mutation around 649026, 649320, 649374.", "psc": { "uniref90_id": "UniRef90_Q9PQQ9", "gene": "rplD", "product": "50S ribosomal protein L4", "uniref50_id": "UniRef50_Q9PQQ9", "cog_id": "COG0088", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022625", "GO:1990904" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQQ9", "UniRef:UniRef50_Q9PQQ9", "COG:COG0088", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022625", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQQ9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQQ9" ], "product": "Large ribosomal subunit protein uL4" } }, "id": "NKIECMJPJD_1123", "locus": "GCA000828735_01120" }, { "type": "cds", "sequence": "contig_1", "start": 649372, "stop": 649566, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAAAAATTAAATTATTATCTATTGATGGTAACTTCGCAAAAGAACTTGAAGTAACCTCTGATCTTTTTGTTGAAGTACCTCACAAACAAGCAATGTTTGACTCAGTGCTTGCTGAAAATGCAGCTGAACGTCAAGGTACACATTCAACCCTAACAAAGGGTGAAGTTCGTGGTGGTGGTAAAAAACCATGA", "aa": "MAKIKLLSIDGNFAKELEVTSDLFVEVPHKQAMFDSVLAENAAERQGTHSTLTKGEVRGGGKKP", "aa_hexdigest": "4721814174a0806d1bd3379485ec955d", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6926.840800000001, "isoelectric_point": 6.71930103302002 }, "id": "NKIECMJPJD_1124", "locus": "GCA000828735_01121" }, { "type": "cds", "sequence": "contig_1", "start": 649577, "stop": 650278, "strand": "-", "frame": 2, "gene": "rplC", "product": "50S ribosomal protein L3", "db_xrefs": [ "COG:COG0087", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022625", "KEGG:K02906", "RefSeq:WP_006688934.1", "SO:0001217", "UniParc:UPI00000C1BDD", "UniRef:UniRef100_Q9PQR0", "UniRef:UniRef50_Q74L88", "UniRef:UniRef90_Q9PQR0" ], "nt": "ATGAAATCATTATTAGGAACAAAAGTTGGCATGACTCAAGTTTTTACAGAAACAGGTAAAGCTGTAGCTGCCACAGTTATCTATGTTGAACCAAATAAGGTTTTAGCTGTTAAAACTAATGAAAAAGACGGCTACAGTGCAATTCAAATTGGTTATGAAACAGTAAAAGAAAAAGCATTAAACAAACCATTATTAGGTCAATTCAAAAAAGCAAATTCAGATCCAAAACGTCACATTAAAGAATTTAGAGATGTTGTTGCTGAAGTTGGTGCTGAATTAACAGTTAGTGAATTTGAACCAGGACAATTAGTTAATGCACAAGCATATACTAAAGGACACGGATTTACAGGTTCAATTAAACGTCATAATTTTAGTATGGGTCCAATGGGCCATGGTGCTGGTTATCCACATCGTTATGTTGGTTCTATTGCTAAAGGACGTGGGGGTAGTCAAGCTCAACGTGTTTTCAAAGGAACTAAATTACCTGGTCATTATGGACATGAATTAGTAACAACTAAAAACTTACTAGTATTAGATGTTAAAGCAAATGAAAATTTAATCCTAATTAAAGGTGCTATTCCAGGACCAAAAGGTTCAATTGTTTTATTAAAATCAGCTAAAAAAGTTGGCCATATCGTAAGCGATCCACAAGTTGTTAACTACTTAGCTAATAAAGCATCATCAAGTGAAGCAAACGAATAA", "aa": "MKSLLGTKVGMTQVFTETGKAVAATVIYVEPNKVLAVKTNEKDGYSAIQIGYETVKEKALNKPLLGQFKKANSDPKRHIKEFRDVVAEVGAELTVSEFEPGQLVNAQAYTKGHGFTGSIKRHNFSMGPMGHGAGYPHRYVGSIAKGRGGSQAQRVFKGTKLPGHYGHELVTTKNLLVLDVKANENLILIKGAIPGPKGSIVLLKSAKKVGHIVSDPQVVNYLANKASSSEANE", "aa_hexdigest": "366a1842e7e2b4fd9559aab16212d569", "start_type": "ATG", "rbs_motif": "AGGAGG", "ups": { "uniparc_id": "UPI00000C1BDD", "ncbi_nrp_id": "WP_006688934.1", "uniref100_id": "UniRef100_Q9PQR0", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1BDD", "RefSeq:WP_006688934.1", "UniRef:UniRef100_Q9PQR0" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQR0", "uniref90_id": "UniRef90_Q9PQR0" }, "psc": { "uniref90_id": "UniRef90_Q9PQR0", "gene": "rplC", "product": "50S ribosomal protein L3", "uniref50_id": "UniRef50_Q74L88", "cog_id": "COG0087", "cog_category": "J", "kegg_orthology_id": "K02906", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_Q74L88", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q74L88" ], "product": "Large ribosomal subunit protein uL3" }, "genes": [ "rplC" ], "id": "NKIECMJPJD_1125", "locus": "GCA000828735_01122" }, { "type": "cds", "sequence": "contig_1", "start": 650303, "stop": 650608, "strand": "-", "frame": 2, "gene": "rpsJ", "product": "30S ribosomal protein S10", "db_xrefs": [ "COG:COG0051", "COG:J", "GO:0000049", "GO:0003723", "GO:0003735", "GO:0005840", "GO:0006412", "GO:1990904", "RefSeq:WP_004025692.1", "SO:0001217", "UniParc:UPI0000134BD5", "UniRef:UniRef100_Q9PQR1", "UniRef:UniRef50_O52331", "UniRef:UniRef90_Q9PQR1" ], "nt": "ATGAATCAAGAATTAAGAATTAGATTAGAATCTTATGACCACAGATTATTAGATGATACAGTAAAAACAATTGTAAACATTTCTAACTCTACAGGTTCTAAATTAAGAGGTCCAATTCCTCTACCAACTAAAAAGGAAATTTTTACAATTTTAAGATCACCACATGTTAATAAATCAAGTCGTGAACAATTTGAAAGAAGAACACACAAACGTTTAATTATTTTAGAAAATCCACAACCTAAAACAATGGAAGCTTTAAAACGTTTATCAGTTCCATTTGGTGTTGAAGTTACTTTCAAAATTTAA", "aa": "MNQELRIRLESYDHRLLDDTVKTIVNISNSTGSKLRGPIPLPTKKEIFTILRSPHVNKSSREQFERRTHKRLIILENPQPKTMEALKRLSVPFGVEVTFKI", "aa_hexdigest": "41422808dcdb5644ddba279f64f59de0", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0000134BD5", "ncbi_nrp_id": "WP_004025692.1", "uniref100_id": "UniRef100_Q9PQR1", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000134BD5", "RefSeq:WP_004025692.1", "UniRef:UniRef100_Q9PQR1" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQR1", "uniref90_id": "UniRef90_Q9PQR1" }, "psc": { "uniref90_id": "UniRef90_Q9PQR1", "gene": "rpsJ", "product": "30S ribosomal protein S10", "uniref50_id": "UniRef50_O52331", "cog_id": "COG0051", "cog_category": "J", "go_ids": [ "GO:0000049", "GO:0003723", "GO:0003735", "GO:0005840", "GO:0006412", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_O52331", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_O52331" ], "product": "Small ribosomal subunit protein uS10" }, "genes": [ "rpsJ" ], "id": "NKIECMJPJD_1126", "locus": "GCA000828735_01123" }, { "type": "cds", "sequence": "contig_1", "start": 650827, "stop": 650955, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGCATTAGTAAAAGCTAATGTTGTTACTAAAGATGGTCAAGGTTTAAATAGAAAAATGCTATCTGAATTAGCAATTAACAACCCTGAAGCATTTAATCAATTAGTTGATAAAGTAATGAAATAA", "aa": "MNALVKANVVTKDGQGLNRKMLSELAINNPEAFNQLVDKVMK", "aa_hexdigest": "3a50490a8e7b9bdc4c3131dbc2c6ec2f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4643.412300000002, "isoelectric_point": 9.398732948303223 }, "id": "NKIECMJPJD_1127", "locus": "GCA000828735_01124" }, { "type": "cds", "sequence": "contig_1", "start": 651244, "stop": 651438, "strand": "-", "frame": 3, "gene": "rpmI", "product": "50S ribosomal protein L35", "db_xrefs": [ "COG:COG0291", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625", "KEGG:K02916", "RefSeq:WP_006688895.1", "SO:0001217", "UniParc:UPI000013400C", "UniRef:UniRef100_Q9PQR3", "UniRef:UniRef50_Q9PQR3", "UniRef:UniRef90_Q9PQR3" ], "nt": "ATGGCTAAAATTAGACAAAAAACAAAAAGAGCTGTTGCAAAACGTTTTAGTATTACAAAAAATGGTAAATTAAAACGTAAACACGCTTATAGATCACATTTAGCTCTAGGTCGTTCAACAAAAGCTAAACGTCATTTAAGAAAAGACGCAATTATGAGCACATCTGACACAAAAAGATATACTCAATGCTTATAG", "aa": "MAKIRQKTKRAVAKRFSITKNGKLKRKHAYRSHLALGRSTKAKRHLRKDAIMSTSDTKRYTQCL", "aa_hexdigest": "2c6112c937e09ff084d377167275f808", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI000013400C", "ncbi_nrp_id": "WP_006688895.1", "uniref100_id": "UniRef100_Q9PQR3", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013400C", "RefSeq:WP_006688895.1", "UniRef:UniRef100_Q9PQR3" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQR3", "uniref90_id": "UniRef90_Q9PQR3" }, "psc": { "uniref90_id": "UniRef90_Q9PQR3", "gene": "rpmI", "product": "50S ribosomal protein L35", "uniref50_id": "UniRef50_Q9PQR3", "cog_id": "COG0291", "cog_category": "J", "kegg_orthology_id": "K02916", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQR3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQR3" ], "product": "Large ribosomal subunit protein bL35" }, "genes": [ "rpmI" ], "id": "NKIECMJPJD_1128", "locus": "GCA000828735_01125" }, { "type": "cds", "sequence": "contig_1", "start": 651453, "stop": 651614, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATTGAAGCACGTGGACGTATGTGTACAAAAGATGAATTTATTGTACAAGCTTATGAAAAATTCATTGACTTAATTAAAGATTATGGTACAGTTGTTCAAGCTAATAAAAAAGTTAGCAATTATCGATATGAAACAATTATTGAACCAATTAAAAAATAA", "aa": "MIEARGRMCTKDEFIVQAYEKFIDLIKDYGTVVQANKKVSNYRYETIIEPIKK", "aa_hexdigest": "ed2087ecde2ff5f320c37dde4c9d57ef", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6288.274900000001, "isoelectric_point": 8.786992454528807 }, "id": "NKIECMJPJD_1129", "locus": "GCA000828735_01126" }, { "type": "cds", "sequence": "contig_1", "start": 651642, "stop": 652016, "strand": "-", "frame": 1, "gene": "infC", "product": "translation initiation factor IF-3", "db_xrefs": [ "COG:COG0290", "COG:J", "GO:0003743", "GO:0005829", "GO:0006413", "GO:0016020", "GO:0032790", "GO:0043022", "SO:0001217", "UniRef:UniRef50_Q9PQR4", "UniRef:UniRef90_Q9PQR4" ], "nt": "ATGAATACACCAAACAATCAACGCCATATGTCATCAAATAATGATGCACGTAAAAATCAACCTTTAATTAATGATCAAATTCGTTTTCGAACAATGGTTGTAATTGATGACCATGGTAATAATTTAGGTGAAATGAATCGTATTGATGCCCTTAATTTAGCCGCTTCAAAGAATTTAGATTTAGTTGTTATTGCTAAAAAAGGAAATATTCCTGTAACAAAAATTTTAGATTATGGTAAGTACAAATACGAACAAAAACGTCGTCAAAAAGAATCAAGAAAAAATCAAACAATTATTAAGGTAAAAGAAATCAAAATTAAACCAATGATTGGTGAACATGATTTAAAAGTAAGAGCAGAAAATGCTAAACGTTGA", "aa": "MNTPNNQRHMSSNNDARKNQPLINDQIRFRTMVVIDDHGNNLGEMNRIDALNLAASKNLDLVVIAKKGNIPVTKILDYGKYKYEQKRRQKESRKNQTIIKVKEIKIKPMIGEHDLKVRAENAKR", "aa_hexdigest": "53a4b9940c46664ac6d058ca6b19f983", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [ "infC" ], "truncated": "5-prime", "pseudogene": { "start": 651453, "stop": 652016, "observations": { "mutation": [ 651644 ], "stop": [ 651644 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQR4", "query_cov": 1.0, "subject_cov": 0.6631016042780749, "identity": 0.992, "score": 235.0, "evalue": 2.45e-77, "gene_start": 1, "gene_end": 124, "reference_start": 1, "reference_end": 124, "reference_sequence": "MNTPNNQRHMSSNNDARKNQPLINDQIRFRTMVVIDDHGNNLGEMNRIDALNLATSKNLDLVVIAKKGNIPVTKILDYGKYKYEQKRRQKESRKNQTIIKVKEIKIKPMIGEHDLKVRAENAKRWLEDKDNVKFVIEARGRMCTKDEFILQAYEKFIDLIKDYGTVVQANKKVSNYRYETIIEPIKK" }, "paralog": false, "identity": 0.983957219251337, "score": 367.0, "evalue": 9.21e-132, "description": "Internal stop codon at 651644. Nonsense mutation around 651644.", "psc": { "uniref90_id": "UniRef90_Q9PQR4", "gene": "infC", "product": "translation initiation factor IF-3", "uniref50_id": "UniRef50_Q9PQR4", "cog_id": "COG0290", "cog_category": "J", "go_ids": [ "GO:0003743", "GO:0005829", "GO:0006413", "GO:0016020", "GO:0032790", "GO:0043022" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQR4", "UniRef:UniRef50_Q9PQR4", "COG:COG0290", "COG:J", "GO:0003743", "GO:0005829", "GO:0006413", "GO:0016020", "GO:0032790", "GO:0043022" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQR4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQR4" ], "product": "Translation initiation factor IF-3" } }, "id": "NKIECMJPJD_1130", "locus": "GCA000828735_01127" }, { "type": "cds", "sequence": "contig_1", "start": 652367, "stop": 652492, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAGGTGGATTAGTTTATGTTGATCAAAAAAATGAACTATATCCATATGAATTATCTAATTCATTTTATTTAGAAAAAGATCCAAATAAACGTCAAGCTTCAAGAGCTTTTTATAATTATTGA", "aa": "MLGGLVYVDQKNELYPYELSNSFYLEKDPNKRQASRAFYNY", "aa_hexdigest": "3b87588ec6e890487aa5a8bdc36128a3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4925.442700000002, "isoelectric_point": 6.037346839904784 }, "id": "NKIECMJPJD_1131", "locus": "GCA000828735_01128" }, { "type": "cds", "sequence": "contig_1", "start": 652541, "stop": 653050, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGCTGGACCTCAAATCGATTATGCAACAACAGCTGCTGCTGAATCAAGTAAACCAATCGTTGTTGTTGGTGTTGATACAGAACAAGAATTAGATGATAATACAAATAAAGCACGTATTAGTGAAAATAATAAATCATTAGCTAACGGTAAAACAATTATTTTTTCTATTGTTAAACGTTTAGATTTAGCTTTTAAAGGTGCTTTATTAAAAGCATCAGAAGGGGCACAATTAACTAATGATATTAACAAAGACGCTTATAAATTAGGAACACACACTGAAGCAAGTTTTAATAAAAATACTTATGTTGATAATACAGCGTTAGTTGAATTATCAAAAGCTGGACATCAGTATTTAATTGATGCAATTAAATTAAGTGGTTTAAAAGAAGTAAATGATTATAAAACAATTGTTGAAATAATCCAAGAAGATCCTTTATTTAAACTTTTATCACAAATTGGAACTAAAAAATTAGATGAAGTTGCTACTAAATCACAACAAGGTGATTGA", "aa": "MAGPQIDYATTAAAESSKPIVVVGVDTEQELDDNTNKARISENNKSLANGKTIIFSIVKRLDLAFKGALLKASEGAQLTNDINKDAYKLGTHTEASFNKNTYVDNTALVELSKAGHQYLIDAIKLSGLKEVNDYKTIVEIIQEDPLFKLLSQIGTKKLDEVATKSQQGD", "aa_hexdigest": "e825a43294b277304cac6a4d2cac82cd", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18372.498199999987, "isoelectric_point": 5.269963645935058 }, "id": "NKIECMJPJD_1132", "locus": "GCA000828735_01129" }, { "type": "cds", "sequence": "contig_1", "start": 653234, "stop": 653767, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGAAAAATTAAATTAAATAAAAAAATTATTTTAGCATCAGTTATTGGAATAATGGGTGTAGCTTTGGTTCCTATAGCATTGGTTGCATGTAGTCAAAATAAAACAAAATCTGATTCTAGAAACTTAACTTTAAACAATTTTTATGGTCGCCCATCAAGTGATGGGGATGATGCATATCAAGCAATCTATGCGTCTAAAATTCAAGATGGTGCACGTATGTTAGGATTAATTTCATTTAGACATAAAAATCCAATTTCAAAATACTTTAATTCACCAAAAGATAATCAACAAGTTTCAGCAGTATTAATTGATGAAATTTATGATTTACAAACAGGTAAAGATCGAATTGCATCAATCACATATCGTGCAGATCAAGCTGCTTTTTTAGCTGGAATTGCTGCAGCTTATTATTTAAATAGTAATCAAAATGTTTTTGGTAAAGATAATAAATTAACATGGGGCGGTTTTGTTGGTATTCATTTACCATCAACTACAAGATTTATTCAAGGTTTTAAATTTGGTATTCAATGA", "aa": "MRKIKLNKKIILASVIGIMGVALVPIALVACSQNKTKSDSRNLTLNNFYGRPSSDGDDAYQAIYASKIQDGARMLGLISFRHKNPISKYFNSPKDNQQVSAVLIDEIYDLQTGKDRIASITYRADQAAFLAGIAAAYYLNSNQNVFGKDNKLTWGGFVGIHLPSTTRFIQGFKFGIQ", "aa_hexdigest": "6617669230e6b2ccab790585dad83a0f", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19528.200099999984, "isoelectric_point": 9.765622520446776 }, "id": "NKIECMJPJD_1133", "locus": "GCA000828735_01130" }, { "type": "cds", "sequence": "contig_1", "start": 653942, "stop": 654184, "strand": "-", "frame": 2, "gene": null, "product": "Unique hypothetical membrane lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQR6", "UniRef:UniRef90_Q9PQR6" ], "nt": "ATGTATAATGCTGGAGTGGCTTTTTTAGTAATTTTTGCTTTAATTGAAATTGCTAGTTTTGCTTTATTTGTAACAACAACAGTCAATGTTGGTTTAAATTTAAAAAGACAAGAAAACTTTGTCTTGCCATTTGTTTTTGGCATTTTAGGGTGCTTAGGAATAATACCTATAATCGGATCAATTATTATTTATGTACAATTAAATAAATTTTATAAGCAACAAAACGAATCTAGTTTAATATAA", "aa": "MYNAGVAFLVIFALIEIASFALFVTTTVNVGLNLKRQENFVLPFVFGILGCLGIIPIIGSIIIYVQLNKFYKQQNESSLI", "aa_hexdigest": "78d3eb77bfa286256045fae4c3054dad", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 653942, "stop": 654280, "observations": { "mutation": [ 654190 ], "stop": [ 654190 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQR6", "query_cov": 1.0, "subject_cov": 0.7142857142857143, "identity": 1.0, "score": 151.0, "evalue": 4.11e-46, "gene_start": 1, "gene_end": 80, "reference_start": 33, "reference_end": 112, "reference_sequence": "MHLGIYYMVYSYYGSPYLTQDIATTNVVNAWAMYNAGVAFLVIFALIEIASFALFVTTTVNVGLNLKRQENFVLPFVFGILGCLGIIPIIGSIIIYVQLNKFYKQQNESSLI" }, "paralog": false, "identity": 0.9821428571428571, "score": 217.0, "evalue": 1.86e-74, "description": "Internal stop codon at 654190. Nonsense mutation around 654190.", "psc": { "uniref90_id": "UniRef90_Q9PQR6", "product": "Unique hypothetical membrane lipoprotein", "uniref50_id": "UniRef50_Q9PQR6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQR6", "UniRef:UniRef50_Q9PQR6" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQR6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQR6" ], "product": "Unique hypothetical membrane lipoprotein" } }, "id": "NKIECMJPJD_1134", "locus": "GCA000828735_01131" }, { "type": "cds", "sequence": "contig_1", "start": 654188, "stop": 654385, "strand": "-", "frame": 2, "gene": null, "product": "DUF4064 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAP9ACD1", "UniRef:UniRef90_A0AAP9ACD1" ], "nt": "GTGAATCAGAACAAAATTGTTAATAAAAAAGATTTAGATATTGCATTAGCAAATTTAATTTTAAGTATAGGAGTAGTTGTTTTTATAATAATTGGCGCTATTTTAGTGCATCTTGGCATTTATTATATGGTTTACTCATATTATGGATCACCATATTTAACGCAAGATATAGCTACAACAAATGTTGTAAATGCTTGA", "aa": "MNQNKIVNKKDLDIALANLILSIGVVVFIIIGAILVHLGIYYMVYSYYGSPYLTQDIATTNVVNA", "aa_hexdigest": "151fbba6d6dd8524648d5daf7e143038", "start_type": "GTG", "rbs_motif": "AGGA", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 653942, "stop": 654385, "observations": { "mutation": [ 654190 ], "stop": [ 654190 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAP9ACD1", "query_cov": 1.0, "subject_cov": 0.4421768707482993, "identity": 1.0, "score": 120.0, "evalue": 1.92e-33, "gene_start": 1, "gene_end": 65, "reference_start": 1, "reference_end": 65, "reference_sequence": "MNQNKIVNKKDLDIALANLILSIGVVVFIIIGAILVHLGIYYMVYSYYGSPYLTQDIATTNVVNAWAMYNAGVAFLVIFALIEIASFALFVTTTVNVGLNLKRQENFVLPFVFGILGCLGIIPIIGSIIIYVQLNKFYKQQNEPSLI" }, "paralog": false, "identity": 0.9795918367346939, "score": 277.0, "evalue": 4.98e-98, "description": "Internal stop codon at 654190. Nonsense mutation around 654190.", "psc": { "uniref90_id": "UniRef90_A0AAP9ACD1", "product": "DUF4064 domain-containing protein", "uniref50_id": "UniRef50_A0AAP9ACD1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAP9ACD1", "UniRef:UniRef50_A0AAP9ACD1" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAP9ACD1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAP9ACD1" ], "product": "DUF4064 domain-containing protein" } }, "id": "NKIECMJPJD_1135", "locus": "GCA000828735_01132" }, { "type": "cds", "sequence": "contig_1", "start": 654395, "stop": 654550, "strand": "-", "frame": 2, "gene": null, "product": "Lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T0B3", "UniRef:UniRef90_A0AAC9T0B3" ], "nt": "ATGCCAGCACTTAAAGAAGCTTTTAGTACTTTGAAAGAAGAAATTAATAAACAAATCAATAAGAGTGAAAAATTATCTGATGAAGCGTATAAAGCATTAACAGCTTCATTAAAAGTTTCAATTGATGGAATGAAAAAAACATTAGGACAAAATTAA", "aa": "MPALKEAFSTLKEEINKQINKSEKLSDEAYKALTASLKVSIDGMKKTLGQN", "aa_hexdigest": "260e8b35733bf59b046b60fd59987efc", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 654395, "stop": 654712, "observations": { "mutation": [ 654574, 654703 ], "stop": [ 654574, 654703 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAC9T0B3", "query_cov": 1.0, "subject_cov": 0.4811320754716981, "identity": 0.961, "score": 92.0, "evalue": 4.19e-23, "gene_start": 1, "gene_end": 51, "reference_start": 55, "reference_end": 105, "reference_sequence": "MKKWQKILTIALPTSLLVAIPIVVASCSTKSEAQKEFEKTYKEYLSLLDKLASKMPALKEAFSTLKEEINKQINKSEKLSDEAYKTLTTSLKVSIDGMKKTLGQNC" }, "paralog": false, "identity": 0.9619047619047619, "score": 189.0, "evalue": 4.72e-64, "description": "Internal stop codon at 654574, 654703. Nonsense mutation around 654574, 654703.", "psc": { "uniref90_id": "UniRef90_A0AAC9T0B3", "product": "Lipoprotein", "uniref50_id": "UniRef50_A0AAC9T0B3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAC9T0B3", "UniRef:UniRef50_A0AAC9T0B3" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAC9T0B3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T0B3" ], "product": "Lipoprotein" } }, "id": "NKIECMJPJD_1136", "locus": "GCA000828735_01133" }, { "type": "cds", "sequence": "contig_1", "start": 654769, "stop": 655887, "strand": "-", "frame": 3, "gene": null, "product": "Lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T368", "UniRef:UniRef90_A0AAC9T368" ], "nt": "ATGACAACGCCAATTTTATTAATTGGCGGATTATCAACATTGGCATTAACAAGTTGTAAAGACGGAAAAAAATCTAATCCAAAACCAGATAAACCATCAACTATAGAAGAATATAAATTAAGTTTATCAAGTGTTGAAACTGGTTTTAATGAAGCTAAATTAATTTTTGATAATAATGATAGTTTTAAAACATTAGATAATTTTAAAGTTATTTTAAAAAATACTCAAAACACTAATGAAGTTATTGAAGCAAGTAATTTATCTGCAACAATTGAAAATCAAAAATTAGTTTTAAAACTAAATAATTTACAAGACGGTCAAACATATAAAATTAACAAAATTTTATATCAAACAAATAAAGAAGTTATTTTAAGTAATAATCAAGTATTTACTACAAAAACTAAACCTAAAATTGAATATGAAGTACAAGAAAAAATTTTGAAACTTGATGATAAAACATATTATGTTGTTCTAAAAGTTAAAGACCCTCAAAATTTAAATGAACCAACAAAAGAAATTTTAAAATCTGTAACTTTTACTTCAAAAATCTCAAATGTTGCAAATCTTAAAAATGCAATATTAGAAAATCAAAGACCTTACGTTAAAGAAGATTATTCAGAAATTCAAATTAAATTACCAAGAGTACCTGTAATTAGTGAAAAAATAGAAATCACTGCAAACAATTCCTTATTTAAATCATTCACAATTACTGTTGGTGAAAAACATATTAAAAATGTGGAAAAAATCATCGATATCACTAAAAAAGTTTTTGGTAATCAAGAATTAAAATCTATTAGTATTACAAAACAAAATAGTAGCAGTGCCAATATAACATTAAGTTTAAAATTAAATGGTAATTCAATTATTGTTGGTGCTAACAAATTAATTAATAAAAAAGACAAAGCAATAAAATTTAGCTTCTTTGTTAAAAATAAAGCAACAAATACAGTTAAAGAATTTAATTTCAATGAAAAATCAAAACCTATTGGAAGCTATCAATTCACATTAAATGGATTAGATGCTAATAGTGATTTTGTAATTGAAGATATTAGATATGATGATAAATCAATCAAAATTACAGAAGAGCTTTTAGCTAGCTTAAGTTTTTCAACAAAATAA", "aa": "MTTPILLIGGLSTLALTSCKDGKKSNPKPDKPSTIEEYKLSLSSVETGFNEAKLIFDNNDSFKTLDNFKVILKNTQNTNEVIEASNLSATIENQKLVLKLNNLQDGQTYKINKILYQTNKEVILSNNQVFTTKTKPKIEYEVQEKILKLDDKTYYVVLKVKDPQNLNEPTKEILKSVTFTSKISNVANLKNAILENQRPYVKEDYSEIQIKLPRVPVISEKIEITANNSLFKSFTITVGEKHIKNVEKIIDITKKVFGNQELKSISITKQNSSSANITLSLKLNGNSIIVGANKLINKKDKAIKFSFFVKNKATNTVKEFNFNEKSKPIGSYQFTLNGLDANSDFVIEDIRYDDKSIKITEELLASLSFSTK", "aa_hexdigest": "feab9ca6521cfc8157023ba1bf6928d0", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0AAC9T368", "query_cov": 1.0, "subject_cov": 0.9738219895287958, "identity": 0.97, "score": 677.0, "evalue": 7.52e-245, "valid": true, "product": "Lipoprotein", "uniref50_id": "UniRef50_A0AAC9T368" }, "pscc": { "uniref50_id": "UniRef50_A0AAC9T368", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T368" ], "product": "Lipoprotein" }, "genes": [], "id": "NKIECMJPJD_1137", "locus": "GCA000828735_01134" }, { "type": "cds", "sequence": "contig_1", "start": 656093, "stop": 656227, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCAATTATTACAACAAAAACAAGTAATTGTAAGAACAACAAAAGATAAGGCACAAAGAGAATTAGTTGCGAATGAAATGCAATATTTAGCAGCTGAAATCGCAACAAGATTTGCACCAGAAGAAGAACATTAA", "aa": "MQLLQQKQVIVRTTKDKAQRELVANEMQYLAAEIATRFAPEEEH", "aa_hexdigest": "fae5e00c3097670d40cadff766e53ee0", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5156.849699999999, "isoelectric_point": 5.668802833557129 }, "id": "NKIECMJPJD_1138", "locus": "GCA000828735_01135" }, { "type": "cds", "sequence": "contig_1", "start": 656357, "stop": 656518, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGCGTTAATACATTTGCAGTTGTTGCAACTTACTTACCAGCTGCTCCAGAACTATATCCTGGACAAGAAGCTTATTATGTATTTATTTCAATTGCATTACCAATGTTTATATGCACATTATTACTTGGATTATTTATTTATCTAATTAATTATGCTTGA", "aa": "MGVNTFAVVATYLPAAPELYPGQEAYYVFISIALPMFICTLLLGLFIYLINYA", "aa_hexdigest": "55385c5ff3ffba4801ee6e649608e269", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5880.968799999997, "isoelectric_point": 4.0500284194946286 }, "id": "NKIECMJPJD_1139", "locus": "GCA000828735_01136" }, { "type": "cds", "sequence": "contig_1", "start": 656579, "stop": 657010, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTGCATTTTTGCAATTTACATGTTTGGTTATAATTCATTAATGCAATACAAAGGTCAAACAATTAGTGATATTTTAGCTAATGAAATTCGTATTAACCAAGATCAAAAAGAACCAATGGCTTTATTGTCTAATCAAAATTTAGATGAAATTAAACTAATGGCTCGTTCAATTTACGAACATAGTCATGCTCCTGGAGCACAAGCTAATGTTTTACATTTTGATTTTAATAAACATGCTAACAGTGAAGTAAAAGCAATTTATGATAAAATCAAAGATTATATTGGCATTAATGCCGTAGCATCGGGAACACCAGCATCAATTACTAATATTTCATTCATTTCAAACGTTGTTTCACAAATCTCAATGTATTATATTGGATATAACATTTTCTTAATTGGATATCTAAGATATGTCTTTAAAGCTAATTGA", "aa": "MCIFAIYMFGYNSLMQYKGQTISDILANEIRINQDQKEPMALLSNQNLDEIKLMARSIYEHSHAPGAQANVLHFDFNKHANSEVKAIYDKIKDYIGINAVASGTPASITNISFISNVVSQISMYYIGYNIFLIGYLRYVFKAN", "aa_hexdigest": "3c284fb5d529845197657797c0a292e2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16218.436799999987, "isoelectric_point": 6.893739891052246 }, "id": "NKIECMJPJD_1140", "locus": "GCA000828735_01137" }, { "type": "cds", "sequence": "contig_1", "start": 657023, "stop": 657133, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAAACCTTTCAACTGGATTAAATTATGCAATGCGTAAAGCCCACTTCCCAGCATGGGTTACATATCGTTTAAAGAATGGGTCAGCGTTAACAGTAGCAATTTTATGA", "aa": "MSNLSTGLNYAMRKAHFPAWVTYRLKNGSALTVAIL", "aa_hexdigest": "127f57d2d1653575b61a5fb19123d1d2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3996.6589999999997, "isoelectric_point": 10.446408653259276 }, "id": "NKIECMJPJD_1141", "locus": "GCA000828735_01138" }, { "type": "cds", "sequence": "contig_1", "start": 657134, "stop": 658105, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00324.27" ], "nt": "ATGATCTTATTCGTCCTTTCATCCTTTTCATTTGCTTTCGCAGGTGCTGATGGATATAATTATTTTAATACACTTAAGTTGGGAACAACTTTAAATCAAGCTACATCAGTCAACTTTTCAAAAAACTTTAGCAATGGGGCATTATATGGAATTGGTATTGTTGTTGTCGCAGGTCTAATCTTTATGAGTTTATTAGGCCAAAAACGAACTCACCAATTTGGCCAAGTTGCTTTAATCATTGGTATTTTACTACCATTCTTTATTATTTTAGGATTCCAAATTTATACAGTGGCAACAATGCCAGATCCATTATACTTTATTGGAAAACACTTCCAAAAAGAACCACATTTAGACCCAAGTGTAACTGGTACTTCTTATGAATTAAATAAAACTTTCATGTCATCAGTTGTTATGGCATCTTTCATGGCATTTAGTTTTAGCTTACATGGTGTAGAAGTTTCAGCTATTGCTGCTAATCGAATGGATAATCCTTCTAAGAAATATCCACAAGCCATGATGATTGTTGTAATTTTTGCTTTAGTTTGCATTATTATTGGATCAATTATGATTTCAATGACTGTACCAACTTCAACATTATCATTCAATGGTGGATTAGTACAGACAATTATGTTCAACTTATCTGTTGGTGGAATTGATGTTAAACTACCAGATGGCACAGTTGCTCATTATAAAACACTAGCACATGCATACTCAGTAATTGAAACTCAATATGGCACAGCTGCCGCTAACCAAGTTATGTATCAATTTGTGTATGGTCATCCAATTAATACAGGTAGTGACTTAGCACGTTCTGATTTATTAACACTAACATCAGAAGTTAACGATCCTTTAAAAAACCCATCTTTATTTATTTCTGTTATGAATATAGAAACAAATAAAATGGCTATGCAAGGATTACAAGCTATTACTTTCTTCATTGGAATAGGTGTATTTGTTGAAGTTTCTGTTTGA", "aa": "MILFVLSSFSFAFAGADGYNYFNTLKLGTTLNQATSVNFSKNFSNGALYGIGIVVVAGLIFMSLLGQKRTHQFGQVALIIGILLPFFIILGFQIYTVATMPDPLYFIGKHFQKEPHLDPSVTGTSYELNKTFMSSVVMASFMAFSFSLHGVEVSAIAANRMDNPSKKYPQAMMIVVIFALVCIIIGSIMISMTVPTSTLSFNGGLVQTIMFNLSVGGIDVKLPDGTVAHYKTLAHAYSVIETQYGTAAANQVMYQFVYGHPINTGSDLARSDLLTLTSEVNDPLKNPSLFISVMNIETNKMAMQGLQAITFFIGIGVFVEVSV", "aa_hexdigest": "703deed678e1312a6d6bf87dfef5dc37", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00324.27", "name": "AA_permease", "length": 179, "aa_cov": 0.5325077399380805, "hmm_cov": 0.9217877094972067, "evalue": 4.392516688611716e-12, "score": 40.93113327026367, "start": 37, "stop": 208 } ], "seq_stats": { "molecular_weight": 35072.57930000002, "isoelectric_point": 6.6421705245971685 }, "id": "NKIECMJPJD_1142", "locus": "GCA000828735_01139" }, { "type": "cds", "sequence": "contig_1", "start": 658298, "stop": 658510, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTCGTAAGCGATTTATCAAAAATAAAATTAGTTTCAAATATTTTTAGAATAAGAGGTGATAAAAAATTGATAAATAATAAAATAAAAGAAGCAAAAATCAAAAAGAAACTAGAAAAAAACAAAAAAGTTTCTTTAATGGGTTTTGTTTTACTAACAGCTTCAGCAATTATTGCTTTTTATACATTCCCATCACTTTCAACAGCTGGTTGA", "aa": "MVVSDLSKIKLVSNIFRIRGDKKLINNKIKEAKIKKKLEKNKKVSLMGFVLLTASAIIAFYTFPSLSTAG", "aa_hexdigest": "c89efec22412d32057151bd3cd424f59", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7798.3881999999985, "isoelectric_point": 10.390707969665527 }, "id": "NKIECMJPJD_1143", "locus": "GCA000828735_01140" }, { "type": "cds", "sequence": "contig_1", "start": 658653, "stop": 659153, "strand": "-", "frame": 1, "gene": null, "product": "site-specific integrase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00130DC0CA" ], "nt": "ATGAAATATTTTGATGTAATTAGTAAAAATAATTTATATAAAAAAACTGATATCTTAGATGATGATTCATTAGAAATTAAAAAATATAAAACCATTATTAGATTCTTGTTTGAAACAGGAATTAGAGCTCATGAATTATTTTTTCTAGAATCTATTAATAATCGTTTGTATGTGTTAGGTAAAGGAAATAAAAAACGACAAATTTTCTTTGTAAAACAAACTTTTGAACAATTACAAAAGTTTTATGAGAATCTAAAAGGATTTGAAACAACTAAAACATTACGTTTATATATTAAAAAAATTATTGGCAAGAATTTTACTCCCCATTCATTACGTCGTAGCTTTGCTACTTTTATGTTAATTAAAGGGGCAAATCCCAAAACTGTAATGTTACAAATGGGACATGCAAACATTCAAACAACGTTTAGTTATTTAAATTTAAATGAACAAACAAATCGTCGAATTTATAACAAAATTATGTACCAAAATGATGCAGAATAA", "aa": "MKYFDVISKNNLYKKTDILDDDSLEIKKYKTIIRFLFETGIRAHELFFLESINNRLYVLGKGNKKRQIFFVKQTFEQLQKFYENLKGFETTKTLRLYIKKIIGKNFTPHSLRRSFATFMLIKGANPKTVMLQMGHANIQTTFSYLNLNEQTNRRIYNKIMYQNDAE", "aa_hexdigest": "63f743b4fdfb1e456d1f91ec351113e6", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00130DC0CA", "query_cov": 0.9337349397590361, "subject_cov": 0.9393939393939394, "identity": 0.516, "score": 139.0, "evalue": 4.28e-39, "valid": false, "product": "site-specific integrase", "uniref50_id": "UniRef50_UPI00130DC0CA" }, "pscc": { "uniref50_id": "UniRef50_UPI00130DC0CA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00130DC0CA" ], "product": "site-specific integrase" }, "genes": [], "id": "NKIECMJPJD_1144", "locus": "GCA000828735_01141" }, { "type": "cds", "sequence": "contig_1", "start": 659301, "stop": 659405, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGATTTTATTAGATATACTAAAAAAAGAAATTTAAGTCTTAACACAATTCGTACTTATGAATCAGTATTAAAACATTATGAACCTGTTTTAGATTCGTGA", "aa": "MKDFIRYTKKRNLSLNTIRTYESVLKHYEPVLDS", "aa_hexdigest": "62b6a9d856ff04c57deeaea2ea449583", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4159.7647000000015, "isoelectric_point": 9.599294090270995 }, "id": "NKIECMJPJD_1145", "locus": "GCA000828735_01142" }, { "type": "cds", "sequence": "contig_1", "start": 659535, "stop": 661100, "strand": "-", "frame": 1, "gene": "fhs", "product": "Formate--tetrahydrofolate ligase", "db_xrefs": [ "COG:COG2759", "COG:F", "EC:6.3.4.3", "GO:0004329", "GO:0005524", "GO:0035999", "RefSeq:WP_006688872.1", "SO:0001217", "UniParc:UPI00000C1BD6", "UniRef:UniRef100_Q9PQS1", "UniRef:UniRef50_Q9PQS1", "UniRef:UniRef90_Q9PQS1" ], "nt": "ATGGCAAAAATTATTGAAGAAATGATTAATAAAAAAGGGTTGTTAATTGATGATTATGAACTTTATGGCAATGAGATTGCTAAAATTAAATCAATTAAAAAACTAAATAAAAAGGATTCAAAATTAATTGTTATTACCTCAATGAATCCTAATCCAGCTGGTGAAGGTAAAACAACAACTGCTATTGGTTTAGTTGATGCTTTAAATAAACATGGATACAAAGCAATTGGAGCCTTACGAGAGCCATCAATGGGACCAGTTTTTGGTATGAAAGGTACGGGTTCTGGAGGAGGATTATCTTTTTTAAAACCCTTTGATAAAATTAATTTACATTTTAGTGGTGATTTCCATGCGATTACTGCAGCTAATAATTTAATCGTTGCAGTAATAGAAAATGAAATACATAATAGGTCTTCAATGCAAATCGATTCTCAAAAAATTTTAATTAAAAGATGTCTAGATGTTAATGATCGTTCTTTACGTAATATTGAATATGATATTAACCATCAACAAACAAAAAGTGGTTTCAACATTACAGCAGCGAGTGATTTAATGGCATTATTTTGTTTAGCACATGATCATAAAGATTTTGAAGATAAACTTGCGCAAACAATTGTTGCATATAATATAGTAAACCAACCTATAAGAATTTGTGATTTAGAATTAACAAAAGCTATTATGGCAATTTTAGAAGATGCTTTATATGCTAATTTAGTACGAACTAATGAGGATAACCCTGTTTTTGTTCATGGTGGCCCATTTGCTAATATTGCTCATGGTTGTAATAGTATTATTGCAACCAAAAATGCTTTAGCTTTAGGTGATTATGTTGTTACTGAATGTGGTTTTGGTAGCGATTTAGGACTTGAAAAATTTATGAATATCAAGATGGCTAGTTTGAATTTAAAACCAGATTTAATTGGTTTAGTTATTAGTCTAAAATCAATCGCATATCATGCTCAAACAAACGAAAAAGACTACGTTAAACAAGGCTTTGCTAATGTTTTATGTCATATTAATCACATTAAAAAATATAATGTTTCTTTTATTGTTTATATTAATGTCAATACTAATACTGATAGCGAAGAAGACTTATTAACTTTAGAAAAACTATTAGATGAACATCAAATTGAACATGCACGTTCTTATGCTTATAGCTATGGTTCTAAAAAAAGTGAAGAAATAACTAAAAAAACAATTACTTTAACTAATCAAATTAATGATCATGAATTAAAATTAATTTATGATATTAAAGATCATTTATCATATAAATTAAAAAAAATATGTGAAAATGTTTATGGTGCTGATGGATATGAATTAAGCTATGAAGCTAAAGAACAATTGAATCGTTATGAACATTTAGATTTTTATTTATGCATTGCTAAAACACCATATTCAATTTCTGATGATGCAAAATTATTAAATAATCCTAAGAACTTTAAAATTCATATTGAACGCTTTGAAATTAATTATGCTGCAAAATTAATCATTGCTATTACAACAACTATTTATCGTATGCCTGGCTTAAATAAAGAACCAGCGGCCAAAAATTTCGTTATGAAATAA", "aa": "MAKIIEEMINKKGLLIDDYELYGNEIAKIKSIKKLNKKDSKLIVITSMNPNPAGEGKTTTAIGLVDALNKHGYKAIGALREPSMGPVFGMKGTGSGGGLSFLKPFDKINLHFSGDFHAITAANNLIVAVIENEIHNRSSMQIDSQKILIKRCLDVNDRSLRNIEYDINHQQTKSGFNITAASDLMALFCLAHDHKDFEDKLAQTIVAYNIVNQPIRICDLELTKAIMAILEDALYANLVRTNEDNPVFVHGGPFANIAHGCNSIIATKNALALGDYVVTECGFGSDLGLEKFMNIKMASLNLKPDLIGLVISLKSIAYHAQTNEKDYVKQGFANVLCHINHIKKYNVSFIVYINVNTNTDSEEDLLTLEKLLDEHQIEHARSYAYSYGSKKSEEITKKTITLTNQINDHELKLIYDIKDHLSYKLKKICENVYGADGYELSYEAKEQLNRYEHLDFYLCIAKTPYSISDDAKLLNNPKNFKIHIERFEINYAAKLIIAITTTIYRMPGLNKEPAAKNFVMK", "aa_hexdigest": "95554c84b10f5fa38bc8255937f3592f", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "ups": { "uniparc_id": "UPI00000C1BD6", "ncbi_nrp_id": "WP_006688872.1", "uniref100_id": "UniRef100_Q9PQS1", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1BD6", "RefSeq:WP_006688872.1", "UniRef:UniRef100_Q9PQS1" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQS1", "uniref90_id": "UniRef90_Q9PQS1" }, "psc": { "uniref90_id": "UniRef90_Q9PQS1", "gene": "fhs", "product": "Formate--tetrahydrofolate ligase", "ec_ids": [ "6.3.4.3" ], "uniref50_id": "UniRef50_Q9PQS1", "cog_id": "COG2759", "cog_category": "F", "go_ids": [ "GO:0005524", "GO:0004329", "GO:0035999" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQS1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQS1" ], "product": "Formate--tetrahydrofolate ligase" }, "genes": [ "fhs" ], "id": "NKIECMJPJD_1146", "locus": "GCA000828735_01143" }, { "type": "cds", "sequence": "contig_1", "start": 661115, "stop": 661525, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATATTTGGTGTTTTTGTTGTCAGTTATTTTATGAATTTATATTATAATGCAGCTATAGGAAGTCGTTTTGGTGATTTGATTATAAAAATTCCTGTTGTTATTAATAATCATCTTTCACAAAAAATTAGTTTTAACAAAAATCCATTATGGGCAATGATTGGATTAGGTGCTATTTTAGGATTAACATTTATTATTCATTTATGGTTGGTTTTTAATGCTCATCATCGTATTAATCGTATTCATAGTTATTATGTTAATGAAATCATTACTAGTGAAGAAATAATTTCATTAAAAAAACAAGCAAATCGTCGTGGTCTAGTCATATTTTTAGTGTTAACTGTTATTTTAGGTTTAATTTTTTATTTGATGTATACTATTTTAAAAAGAAAATACACTATCAAAAAATAA", "aa": "MIFGVFVVSYFMNLYYNAAIGSRFGDLIIKIPVVINNHLSQKISFNKNPLWAMIGLGAILGLTFIIHLWLVFNAHHRINRIHSYYVNEIITSEEIISLKKQANRRGLVIFLVLTVILGLIFYLMYTILKRKYTIKK", "aa_hexdigest": "d4b97649a4d75cc7c829e021c713928d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15817.869299999995, "isoelectric_point": 10.14630832672119 }, "id": "NKIECMJPJD_1147", "locus": "GCA000828735_01144" }, { "type": "cds", "sequence": "contig_1", "start": 661559, "stop": 661783, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTTATGAAGTTATTATTAATCATTTTAATCCATGGATTTTTTTACCAATTAGTATTTATTGTGTCTTGATGATTTTCTTTTTAGCTTTATCATGTAGTAATTTTATAAATTTTAAAAAAGAAATGGATTATCAAAAAAACCATTTTGATTGTACAAAAGCAACAAATTTTATTGTAGGTATATATCGTAAATTAATAGTAGTACATATTAATGTAAACTGA", "aa": "MIYEVIINHFNPWIFLPISIYCVLMIFFLALSCSNFINFKKEMDYQKNHFDCTKATNFIVGIYRKLIVVHINVN", "aa_hexdigest": "9c9ad2ba479c620605fde1c00c4e8edb", "start_type": "TTG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8850.485399999998, "isoelectric_point": 8.52151165008545 }, "id": "NKIECMJPJD_1148", "locus": "GCA000828735_01145" }, { "type": "cds", "sequence": "contig_1", "start": 661823, "stop": 662401, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E492BA6", "UniRef:UniRef90_UPI001E492BA6" ], "nt": "ATGCCACGAATAAATACGCAAGTTTTTAATGAAAATAATGATAAAAATAGAATTAAAAGTAATAACAATAAAATTTGTGATGATATTGAATACACTCGCTTATTAGAAACGAAAGAATATAATGCTGGTTTTTCTCCTAATAATGATTCACAAGATATCTTAATTAAAAAACAAGACAATATTTTAAAGTTAAAACCAACTCTAAATTTTGATGATAGTGATCAATTAATTTCAATTGTTCCTACTAAAAATTATGAAAATTTAAGATTAAAAGAAATTGAAATCGATAAAAAAAACACTGTTGATTATCAATTAAAAAAAGAAATAATAGATCAGGACTTAACAGATGATAATCATTTATCTATAAGTCAAAAATCCAATGGTTTTGCTTTAAAAACTAAACAATTTATTCCTCAAAATATTTCAGAATTTATTTATCATAATGAAGACGATCCAAAAACATACGGAAGTGAAAATAATTTAACTTTTAGTCAACTAGAAGAAAAAGTTAATCAAAAAAATACACCTGATTTTTATTTAAAAGAAATTCATGCAGTTCGTAACAAAATTATCATTTGA", "aa": "MPRINTQVFNENNDKNRIKSNNNKICDDIEYTRLLETKEYNAGFSPNNDSQDILIKKQDNILKLKPTLNFDDSDQLISIVPTKNYENLRLKEIEIDKKNTVDYQLKKEIIDQDLTDDNHLSISQKSNGFALKTKQFIPQNISEFIYHNEDDPKTYGSENNLTFSQLEEKVNQKNTPDFYLKEIHAVRNKIII", "aa_hexdigest": "ab6c3d316eaf2ede2c92efc5a34a7d5c", "start_type": "ATG", "rbs_motif": "GGxGG", "psc": { "uniref90_id": "UniRef90_UPI001E492BA6", "query_cov": 1.0, "subject_cov": 0.803347280334728, "identity": 1.0, "score": 299.0, "evalue": 1.18e-100, "valid": true, "uniref50_id": "UniRef50_UPI001E492BA6" }, "pscc": { "uniref50_id": "UniRef50_UPI001E492BA6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E492BA6" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 22541.922800000008, "isoelectric_point": 5.325950050354003 }, "id": "NKIECMJPJD_1149", "locus": "GCA000828735_01146" }, { "type": "cds", "sequence": "contig_1", "start": 662628, "stop": 663047, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGTGTTTTAGTATGTTATTCAGTTTCTAGATTATTAGATTACTTCCGTTTTGATGATTCTTTAGAAGTTCTACCAGTTCATGGAATGGGTGGTGTTATAGGTTCTTTATTAATTGGAGCATTTGCTACTAACACGGTTGCACATGGAATTAAATATGACGCCGTCGGATTAGATAATAATGGAAATCCAATTTATGGTATTTTATTTGGACTACAATTAGGTGCTGTATTATTAGCAGCAACATGGGCATTTGCCTTTACATGTTTAATAGTTTATATTACTAAACCTCGTTTATCAGTGCGTCAACAATTAGGCCATATAGACTACATCAATCATGGTGAAGATGCATACAAGTTTGATTTTATTATTCCTTCAGAAAGTGAAATGTTTGAATATGAAAGTTTTGATCAAACTTGA", "aa": "MGVLVCYSVSRLLDYFRFDDSLEVLPVHGMGGVIGSLLIGAFATNTVAHGIKYDAVGLDNNGNPIYGILFGLQLGAVLLAATWAFAFTCLIVYITKPRLSVRQQLGHIDYINHGEDAYKFDFIIPSESEMFEYESFDQT", "aa_hexdigest": "9f710d3c9c266d8d45c6136afcff77f8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15379.43589999998, "isoelectric_point": 4.652578544616699 }, "id": "NKIECMJPJD_1150", "locus": "GCA000828735_01147" }, { "type": "cds", "sequence": "contig_1", "start": 663049, "stop": 663255, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAATAGGAACAGAGGCTCAAACTGGAACAAAACCTGCTGCGGGAGTAATTGTTGCTAATCCAGCGACTGCACCAGTCACTAAACCTACTGTTGTTGGTTTGTGGTTTTTGTTAACAATAGTTTCAAGTGTAACTCAACCTATCATACCTATTGCTAAGGCAATAATTGTATTTAATCATGATGATATAGCTTGTGAAATGTGA", "aa": "MAIGTEAQTGTKPAAGVIVANPATAPVTKPTVVGLWFLLTIVSSVTQPIIPIAKAIIVFNHDDIACEM", "aa_hexdigest": "4c76495ed011e12787d313652241528a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7014.230699999999, "isoelectric_point": 5.464580345153809 }, "id": "NKIECMJPJD_1151", "locus": "GCA000828735_01148" }, { "type": "cds", "sequence": "contig_1", "start": 663303, "stop": 663398, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAGGAAAACGTACAACATTAATTACAGATAAAAAGCGAGCTAATAATTTACCAATGGTTGTATTAGGTGCAGCATTACTATTTTTTGGTTGA", "aa": "MLGKRTTLITDKKRANNLPMVVLGAALLFFG", "aa_hexdigest": "5c323901354bd9707921ba57a4fe3b20", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3390.1155000000003, "isoelectric_point": 11.097023582458494 }, "id": "NKIECMJPJD_1152", "locus": "GCA000828735_01149" }, { "type": "cds", "sequence": "contig_1", "start": 663807, "stop": 664010, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCCCGCAGATGCAAGTCAATCAACACTTAATGCAATAGGTTTTAATCCTACTAATACACTACTAGTAGCTTTAGCAATTGCTTTTGTTTTACTAATGACACCAGGGTTAGCATTATTTTATGGTGGTTTAGTAAGACGTAAATCAGCTTTAACAATTATTAATCAATGTGTAGCTTCACTTGGAATTACAACATTGATTTGA", "aa": "MPADASQSTLNAIGFNPTNTLLVALAIAFVLLMTPGLALFYGGLVRRKSALTIINQCVASLGITTLI", "aa_hexdigest": "12074291a7c50eb8309489cc66b713b2", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6962.2451999999985, "isoelectric_point": 9.301901435852049 }, "id": "NKIECMJPJD_1153", "locus": "GCA000828735_01150" }, { "type": "cds", "sequence": "contig_1", "start": 664319, "stop": 664636, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGTGGTGTTACTGGTTCATTATTAATTGGTGCGTTTGCATCTAAATCTGTAAACCCATCAATTATGTATGAAGCAATTGGTCCAACAACAACTGGTGTTTTATTTGGTGTCCAATTAGGAGCGGTTTTATTAGCTGCGGTTTATGGATTTGTATTTACTATTTTATTAGTATATATTACTCGTCCAAGATTATCAGCACGCCAACAATTAGGTCATATAGACTATATTAATCATGGCGAAGATGCATATTCATTCGATATTGAAATTCCATCTGAAAAAGAAATGGAGCAATATGAATTAGCAGATAGTACATGA", "aa": "MGGVTGSLLIGAFASKSVNPSIMYEAIGPTTTGVLFGVQLGAVLLAAVYGFVFTILLVYITRPRLSARQQLGHIDYINHGEDAYSFDIEIPSEKEMEQYELADST", "aa_hexdigest": "186eb1fd867902a06997d4c47e2b15ec", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11346.833299999995, "isoelectric_point": 4.562602424621583 }, "id": "NKIECMJPJD_1154", "locus": "GCA000828735_01151" }, { "type": "cds", "sequence": "contig_1", "start": 665489, "stop": 665692, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCCAGCAACAGCTGATGCTAATACATTAGCATTAACTAATTTTAATCCGACTAGCACTTTATTAGTTGCATTAGCAATTGCCTTTGTCTTATTGATGACTCCAGGTTTAGCATTATTTTATGGTGGGCTAACACGTCGAAAATCTACATTAACGATTATTAATCAATGTGTAGCTTCACTTGGAATCACAACACTGATTTGA", "aa": "MPATADANTLALTNFNPTSTLLVALAIAFVLLMTPGLALFYGGLTRRKSTLTIINQCVASLGITTLI", "aa_hexdigest": "e2a728ad2b50d61580bd48a565ecd5fe", "start_type": "GTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6995.271999999998, "isoelectric_point": 9.301901435852049 }, "id": "NKIECMJPJD_1155", "locus": "GCA000828735_01152" }, { "type": "cds", "sequence": "contig_1", "start": 666000, "stop": 666821, "strand": "-", "frame": 1, "gene": "rsgA", "product": "ribosome small subunit-dependent GTPase A", "db_xrefs": [ "COG:COG1162", "COG:J", "EC:3.6.1.-", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0006412", "GO:0019843", "GO:0042274", "GO:0043022", "GO:0046872", "SO:0001217", "UniRef:UniRef50_Q9PQS5", "UniRef:UniRef90_Q9PQS5" ], "nt": "GTGCGGGCAAAGATTATTTCGGTTGTTGCTAATAATTTTTATGTACATCTTTATGATTTAAAAATTCAGATAAAAGCAATTCCCAAAGGGATTTTTAAACATAACAGTCATGAATTAAAACCAATGGTTGGGGATGATGTTGAAGTTGAATTAATTGGGAATACTTATTTAATCACAAAAATTTATGAACGTTATAATCAATTAATTCGTCCCAAAGTTGCAAATATTGATATTGTTTTAATCGTAGTCTCTATTGTACAACCAGATTTAAATACATTGAGTTTAAACAAATATTTAGCTTTTTATGAAGCTCGCAATATTAAAAATGTTTTAATAGGTTTGAGTAAATATGATTTAGCCAATAATCATTTAAAACAAAAAATTAATAAGTTAATTTTAGATTATAAAAAAAATAACTATGCGGTTTTTATATTAACAAAAGAAAATGATATTTTATTATTAAAAGAAAAGATTAAAAGCAAGACACTTTGTTTGGCAGGAAATTCAGGCGTAGGTAAATCAACATTAATTAATAAATTAGATCCAAGCATTCAACAACGTACACAGGAAATATCACATTTTCTGAATCGAGGAAAACACACAACAACAAGTACCAAACTAATTAGTTTTGCTAATGGCTTTTTAGTTGATACACCGGGTTTTGGAAATTTAGAGGTTAATTTAACCAAGAATGAAATGGCTAATGCCTTTAATGATTTTGCAAATTATGCACGTTTTTGTAAATTTTCTAATTGTTTACATATTGAAGAACCAGAATGTGCTATTAAAAAAGCTGTTGATGAAAAATTAATTGTTAATTGA", "aa": "MRAKIISVVANNFYVHLYDLKIQIKAIPKGIFKHNSHELKPMVGDDVEVELIGNTYLITKIYERYNQLIRPKVANIDIVLIVVSIVQPDLNTLSLNKYLAFYEARNIKNVLIGLSKYDLANNHLKQKINKLILDYKKNNYAVFILTKENDILLLKEKIKSKTLCLAGNSGVGKSTLINKLDPSIQQRTQEISHFLNRGKHTTTSTKLISFANGFLVDTPGFGNLEVNLTKNEMANAFNDFANYARFCKFSNCLHIEEPECAIKKAVDEKLIVN", "aa_hexdigest": "3381ab71c5070bfe1183c1c96eb6fd3d", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_Q9PQS5", "query_cov": 1.0, "subject_cov": 0.900990099009901, "identity": 1.0, "score": 530.0, "evalue": 1.73e-189, "valid": true, "gene": "rsgA", "product": "ribosome small subunit-dependent GTPase A", "ec_ids": [ "3.6.1.-" ], "uniref50_id": "UniRef50_Q9PQS5", "cog_id": "COG1162", "cog_category": "J", "go_ids": [ "GO:0003924", "GO:0005525", "GO:0005737", "GO:0006412", "GO:0019843", "GO:0042274", "GO:0043022", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQS5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQS5" ], "product": "Small ribosomal subunit biogenesis GTPase RsgA" }, "genes": [ "rsgA" ], "id": "NKIECMJPJD_1156", "locus": "GCA000828735_01153" }, { "type": "cds", "sequence": "contig_1", "start": 666926, "stop": 667087, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTATACAAAGATATCTACACTTTAATTGATGATTTAAGTAATGTTATGGATCCAGAGTCAAATCTTAATCAAGAATACATTAAACCATTAAATAAGTTACGAATTTATCGTAAAAATCTAGCAGGCTTATATGAAATTAAAGAAAAAGATCGTAAATGA", "aa": "MLYKDIYTLIDDLSNVMDPESNLNQEYIKPLNKLRIYRKNLAGLYEIKEKDRK", "aa_hexdigest": "9b1e9486c2d1323a2d05da69479def14", "start_type": "TTG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6420.3696, "isoelectric_point": 7.942392158508301 }, "id": "NKIECMJPJD_1157", "locus": "GCA000828735_01154" }, { "type": "cds", "sequence": "contig_1", "start": 667193, "stop": 667984, "strand": "-", "frame": 2, "gene": "sPS1", "product": "Serine/threonine kinase", "db_xrefs": [ "COG:COG0515", "COG:T", "SO:0001217", "UniRef:UniRef50_Q9PQS6", "UniRef:UniRef90_Q9PQS6" ], "nt": "ATGCGTGAAGAAATAAAAACAAACTCAATTCTTAATTATAAATATAAAGTTGTTAAACACCTAGCAGATGGTGGTTTTAGTAAAGTTTATTTATGTTGTTTTTTATCAGATGAAACTAAGTTTATTGTTATTAAAGTTTTAGATATTAATGATGAGAAACAACAAATGGTTGTTTATGATGAATTAAGAATTTCAAATTTAATTAAAAATTCTGATTCTGATAAAAAAAACTATATTATGGAATATTATGAATATTTTGAAACCGGTTCGTTAGAAAATAATGATAAAAGGATTTATATTGTTTTTGAATACATTGAAGGTTTGACATTGCGTGAATATCTTGATGAATTTAAAACAATTACTTATGTTAAGGCTGTTGAAATTATTCGTCAGGTTGCCTTAGGTGTAAGTTTTTTTCATAGTTGTAATCCACAAATTATTCATCGTGATTTAAAGCCAGAAAATTGCATGATTAATAAAACCTTAACAAAAATTAAAATTATTGATTATGGTGCAGCATCGGTTTTTTACAATCGTGAAGATTTAACAAAAGATCAAGAAATTAAATGTACAATTATTTACGCTTCACCAAAATTATTAAGTCTTGGGCAAAAAGTTAAAGAACAAGCTAATAAGGGTTTAAATAAAAGAGCTTTAAGTTTAATTAATGACGCATTAGGTGTTAGTTATGATATTCATTCTTTAGGAGTTATGTTATATGAATTAATTACTGGAACTAACCCTTTTAGTGAACATACAATTACAGATGATCGTGATTATTTAGAAAAATGA", "aa": "MREEIKTNSILNYKYKVVKHLADGGFSKVYLCCFLSDETKFIVIKVLDINDEKQQMVVYDELRISNLIKNSDSDKKNYIMEYYEYFETGSLENNDKRIYIVFEYIEGLTLREYLDEFKTITYVKAVEIIRQVALGVSFFHSCNPQIIHRDLKPENCMINKTLTKIKIIDYGAASVFYNREDLTKDQEIKCTIIYASPKLLSLGQKVKEQANKGLNKRALSLINDALGVSYDIHSLGVMLYELITGTNPFSEHTITDDRDYLEK", "aa_hexdigest": "bbdca21c34a0a0871aa52ff314662f16", "start_type": "ATG", "rbs_motif": null, "genes": [ "sPS1" ], "truncated": "5-prime", "pseudogene": { "start": 666893, "stop": 667984, "observations": { "mutation": [ 666913, 666928, 667108, 667195 ], "stop": [ 666913, 666928, 667108, 667195 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQS6", "query_cov": 1.0, "subject_cov": 0.6813471502590673, "identity": 0.932, "score": 494.0, "evalue": 1.68e-174, "gene_start": 1, "gene_end": 263, "reference_start": 1, "reference_end": 263, "reference_sequence": "MREQIQTNSILNHKYKVVKHLADGGFSKVYLCCFLSDETKFIVVKVLDIGDEKQQMVVYDELRISNLIKNSNSDKRSYIMEYYEYFESGSLETDDKRIYIVFEYIDGLTLREYLDEFKTVTYVKAVEIIRQVALGVSFFHSCNPQIIHRDLKPENCMINKTLSKIKIIDYGAASVFYNREDLTKDQEIKCTIIYASPKLLSLGQKVKEQASKGLNKNALSLINDALGVNYDIHSLGVMLYELITGTNPFSEHTIKDDRDYLEKWTTYDVEPLSSINKTIPKGIDNILIRCFAWKKEDNKLLYKDIYTLIDDLNNVMDPESSLNQDYIKPLNKLRIYRKNVAGLYEIKGKDRKWYLQKWFIILVGCLSIILIILLIIILSFKKSGAI" }, "paralog": false, "identity": 0.9146005509641874, "score": 667.0, "evalue": 7.4e-245, "description": "Internal stop codon at 666913, 666928, 667108, 667195. Nonsense mutation around 666913, 666928, 667108, 667195.", "psc": { "uniref90_id": "UniRef90_Q9PQS6", "gene": "SPS1", "product": "Serine/threonine kinase", "uniref50_id": "UniRef50_Q9PQS6", "cog_id": "COG0515", "cog_category": "T", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQS6", "UniRef:UniRef50_Q9PQS6", "COG:COG0515", "COG:T" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQS6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQS6" ], "product": "Serine/threonine kinase" } }, "id": "NKIECMJPJD_1158", "locus": "GCA000828735_01155" }, { "type": "cds", "sequence": "contig_1", "start": 667990, "stop": 668733, "strand": "-", "frame": 3, "gene": "pTC1", "product": "Protein phosphatase", "db_xrefs": [ "COG:COG0631", "COG:T", "EC:3.1.3.16", "KEGG:K20074", "RefSeq:WP_006688864.1", "SO:0001217", "UniParc:UPI00000C1BD0", "UniRef:UniRef100_Q9PQS7", "UniRef:UniRef50_Q9PQS7", "UniRef:UniRef90_Q9PQS7" ], "nt": "ATGAATTTTGGGTTTATTAGTGATATTGGCTCACAACGCAAACATAATGATGATTGTGCATTGGTAATTCAAAATCAATATCAACAAACTTTATTAATTGTTTGTGATGGATTAGGCGGGTATAAAGGTGGTGCTGCGGCTAGCCACATTACATTAGAAACAATTAAAGATAATTTTTTAACTACAGATTTTAGTGAGTATGATAAACAACAGATTCAAAAATGGTATATTAAAGTTATTAAACTAGCACAGGTTGAAATTGATCGCACTGTTTTACTAGATAAAGACGTTTATAATATGGGAACTACAGTTGTTGCTTCTATTGTTGTTAATGATTTTTCATACACTCTAAATATTGGTGATTCGCGTGCTTATTTATTATCCAATGAACAATTCTTACAAATAAGTCGCGATCATAATTTATTACAAGTTTTACAAGAACGTAAAGCCAACCCTGAGGTTTATAAAAAACATGAGAAGAATTTATTTTCATTAACACAATTTGTTGGTCGCACAAGTAATATTTTTTTGAGTTATGACTTATTCGTAAATAAATTAAACCCAAATGAAATTATTGTTTTAACATCAGATGGATTCCATAACTATTTTGAATTAAATACATTATATGAAAAATTAATAAAAAGCACCCAAGAGACAAAAAATGAACTTTTACAACAATTAATAAGACAAGCTATTATCAACGGTAGCAATGATAATTTATCATTAGCTTTCTTAATTTTTTAA", "aa": "MNFGFISDIGSQRKHNDDCALVIQNQYQQTLLIVCDGLGGYKGGAAASHITLETIKDNFLTTDFSEYDKQQIQKWYIKVIKLAQVEIDRTVLLDKDVYNMGTTVVASIVVNDFSYTLNIGDSRAYLLSNEQFLQISRDHNLLQVLQERKANPEVYKKHEKNLFSLTQFVGRTSNIFLSYDLFVNKLNPNEIIVLTSDGFHNYFELNTLYEKLIKSTQETKNELLQQLIRQAIINGSNDNLSLAFLIF", "aa_hexdigest": "20c79ef7bfabe59bf79301183229558b", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI00000C1BD0", "ncbi_nrp_id": "WP_006688864.1", "uniref100_id": "UniRef100_Q9PQS7", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1BD0", "RefSeq:WP_006688864.1", "UniRef:UniRef100_Q9PQS7" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQS7", "uniref90_id": "UniRef90_Q9PQS7" }, "psc": { "uniref90_id": "UniRef90_Q9PQS7", "gene": "PTC1", "product": "Protein phosphatase", "ec_ids": [ "3.1.3.16" ], "uniref50_id": "UniRef50_Q9PQS7", "cog_id": "COG0631", "cog_category": "T", "kegg_orthology_id": "K20074" }, "pscc": { "uniref50_id": "UniRef50_Q9PQS7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQS7" ], "product": "Protein phosphatase" }, "genes": [ "pTC1" ], "id": "NKIECMJPJD_1159", "locus": "GCA000828735_01156" }, { "type": "cds", "sequence": "contig_1", "start": 668737, "stop": 668895, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCTTTTAATTCCTTATAATTTAAATAATTATCGAACTAGTGCTAATTTAGATGATTTTTTTAATATTTTATCTGTTAATAAAATACGAATTAGTAAGCGTATACGAAAAAGTAACGATTTAGATATTAGTTTTAAACAATTGAAAATTAAGGAGTAA", "aa": "MLLIPYNLNNYRTSANLDDFFNILSVNKIRISKRIRKSNDLDISFKQLKIKE", "aa_hexdigest": "af46c081bbcbd00ae9b6fac296164635", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6215.187599999999, "isoelectric_point": 9.991777610778808 }, "id": "NKIECMJPJD_1160", "locus": "GCA000828735_01157" }, { "type": "cds", "sequence": "contig_1", "start": 669178, "stop": 669747, "strand": "-", "frame": 3, "gene": "rlmN", "product": "Dual-specificity RNA methyltransferase RlmN N-terminal domain-containing protein", "db_xrefs": [ "COG:COG0820", "COG:J", "EC:2.1.1.192", "KEGG:K06941", "SO:0001217", "UniRef:UniRef50_A0AAC9X6G4", "UniRef:UniRef90_A0AAC9X6G4" ], "nt": "ATGAAATTAAACAATCATAATCTAAAAGAAATTTATTCATTATCATTAAATGAACTTAAAGAAGAATTATTTAAATTAGGCTTAAAATCTTTTATTAGCAAACAAATTTATTCTTGGATTTATCAGAAACGAATTTTTAATTTTGATAAATTTTCTAATATTGCTAAAAATAATGTAATAATTCTAAAAGAAAATTTTAGTAATAATCTATTAAAAATTAACAGTTATCAATCAAATTCTGATGGTTCAATTAAGTTTGAATTTTTAACAGATAAAAATCTGATTATTAATTGCATGATTATTAAATTTAAAAACGGATTTTTAATAAAAATAAATCCGTTTGGAATTAATTTTAAAAAAGAAATTATTAATTTATCAACTAATGAATTAGTATTACAAATTTTATTAATACAACAATTTCTAGATCAACGTAAATTAGGCAACATTACTAATGTTATTATAAAAGGATTACAGGATAGCTTATTAAATATGGACGTTGTATCAAACTTCATTAATATTATTAATAATGAAAATGGTTTAAATATTGGAAAAAGGAAAATTGCAGTTTGA", "aa": "MKLNNHNLKEIYSLSLNELKEELFKLGLKSFISKQIYSWIYQKRIFNFDKFSNIAKNNVIILKENFSNNLLKINSYQSNSDGSIKFEFLTDKNLIINCMIIKFKNGFLIKINPFGINFKKEIINLSTNELVLQILLIQQFLDQRKLGNITNVIIKGLQDSLLNMDVVSNFINIINNENGLNIGKRKIAV", "aa_hexdigest": "f54a92f590ae3445dc5b2fb812cdb437", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "genes": [ "rlmN" ], "truncated": "5-prime", "pseudogene": { "start": 668875, "stop": 669747, "observations": { "mutation": [ 669048, 669147, 669180 ], "stop": [ 669048, 669147, 669180 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAC9X6G4", "query_cov": 1.0, "subject_cov": 0.5625, "identity": 1.0, "score": 358.0, "evalue": 7.5e-123, "gene_start": 1, "gene_end": 189, "reference_start": 1, "reference_end": 189, "reference_sequence": "MKLNNHNLKEIYSLSLNELKEELFKLGLKSFISKQIYSWIYQKRIFNFDKFSNIAKNNVIILKENFSNNLLKINSYQSNSDGSIKFEFLTDKNLIINCMIIKFKNGFLIKINPFGINFKKEIINLSTNELVLQILLIQQFLDQRKLGNITNVIIKGLQDSLLNMDVVSNFINIINNENGLNIGKRKIAVWTSGINVDLIKWGQLQNQIELIISMNASNSLIYQKIMLKKLNQNWSFLKLIEQIKAYTKITNNRVVLEYLLIDKINDDLNYANELVELLKNILCYVLLIPYNLNNYRTSANLDDFFNILSVNKIRISKRIRKSNDLDISFKQLKIKE" }, "paralog": false, "identity": 0.9896551724137931, "score": 551.0, "evalue": 4.36e-201, "description": "Internal stop codon at 669048, 669147, 669180. Nonsense mutation around 669048, 669147, 669180.", "psc": { "uniref90_id": "UniRef90_A0AAC9X6G4", "gene": "RlmN", "product": "Dual-specificity RNA methyltransferase RlmN N-terminal domain-containing protein", "ec_ids": [ "2.1.1.192" ], "uniref50_id": "UniRef50_A0AAC9X6G4", "cog_id": "COG0820", "cog_category": "J", "kegg_orthology_id": "K06941", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAC9X6G4", "EC:2.1.1.192", "UniRef:UniRef50_A0AAC9X6G4", "COG:COG0820", "COG:J", "KEGG:K06941" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAC9X6G4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9X6G4" ], "product": "Dual-specificity RNA methyltransferase RlmN N-terminal domain-containing protein" } }, "id": "NKIECMJPJD_1161", "locus": "GCA000828735_01158" }, { "type": "cds", "sequence": "contig_1", "start": 670085, "stop": 670303, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00625.27" ], "nt": "ATGAAACGCGGAAAATTAATTGTTTTTAGTGGACCTAGTGGTGTAGGAAAACATACAATTCTATCTAAAATCATAAATCGTAAAGATTTAAATTTAGCATATAGTATTTCAATGACAACCCGAAAAAAACGTGAAGGTGAAGTTAATGGTGTTGATTATTATTTTGTTAATGATGAAGAATTTAAAAAGGCCATACTAAATAATGAATTAATTGAGTGA", "aa": "MKRGKLIVFSGPSGVGKHTILSKIINRKDLNLAYSISMTTRKKREGEVNGVDYYFVNDEEFKKAILNNELIE", "aa_hexdigest": "6e9b31d227d796d53c80bf9e2c1c25e4", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00625.27", "name": "Guanylate_kin", "length": 69, "aa_cov": 0.9583333333333334, "hmm_cov": 1.0, "evalue": 4.9802350068948434e-23, "score": 77.28531646728516, "start": 4, "stop": 72 } ], "seq_stats": { "molecular_weight": 8218.4237, "isoelectric_point": 9.520965003967284 }, "id": "NKIECMJPJD_1162", "locus": "GCA000828735_01159" }, { "type": "ncRNA-region", "class": [ "attenuator", "SO:0000140" ], "sequence": "contig_1", "start": 670518, "stop": 670593, "strand": "+", "label": "L21_leader", "product": "Ribosomal protein L21 leader", "score": 60.1, "evalue": 1.7e-07, "db_xrefs": [ "RFAM:RF00559", "SO:0000140" ], "nt": "ATGCAGCTTCAACCACTCAAAAAAGGTTTAAAAATAAATGTTAATTTATACCTTATTGGTGAGTCTAAGTTGATAA", "id": "NKIECMJPJD_41" }, { "type": "cds", "sequence": "contig_1", "start": 670621, "stop": 670923, "strand": "+", "frame": 1, "gene": "rplU", "product": "50S ribosomal protein L21", "db_xrefs": [ "COG:COG0261", "COG:J", "GO:0003723", "GO:0003735", "GO:0005737", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904", "KEGG:K02888", "RefSeq:WP_006688851.1", "SO:0001217", "UniParc:UPI00000C1BCE", "UniRef:UniRef100_Q9PQT0", "UniRef:UniRef50_Q9PQT0", "UniRef:UniRef90_Q9PQT0" ], "nt": "ATGTTTGCTATTTTTCAAACAGGCGGAAAACAATACAAAGTTCAACAAGGCGAAAAAATCTATGTTGAAAAACTAGATTTAGAAGTTGGTAGTAAAATTTCATTTGATCAAGTTATTATGGTAGAAGGTAGTGTTGGTACTCCTTTTGTAAAAAATGCTGTAGTTAATGCTACTGTTATAAAACAAGGAAAACAAAAGAAAATCAACATTATTAAATTTAAATCTAAAAAACACCATTTAAAACGTCAAGGTCATCGACAACCATATACACAATTAGTTATTGATTCAATTAGTGTTAAGTAG", "aa": "MFAIFQTGGKQYKVQQGEKIYVEKLDLEVGSKISFDQVIMVEGSVGTPFVKNAVVNATVIKQGKQKKINIIKFKSKKHHLKRQGHRQPYTQLVIDSISVK", "aa_hexdigest": "41b89912f33dbdcdcb2aad6b7e65a98c", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI00000C1BCE", "ncbi_nrp_id": "WP_006688851.1", "uniref100_id": "UniRef100_Q9PQT0", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1BCE", "RefSeq:WP_006688851.1", "UniRef:UniRef100_Q9PQT0" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQT0", "uniref90_id": "UniRef90_Q9PQT0" }, "psc": { "uniref90_id": "UniRef90_Q9PQT0", "gene": "rplU", "product": "50S ribosomal protein L21", "uniref50_id": "UniRef50_Q9PQT0", "cog_id": "COG0261", "cog_category": "J", "kegg_orthology_id": "K02888", "go_ids": [ "GO:0003723", "GO:0003735", "GO:0005737", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQT0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQT0" ], "product": "Large ribosomal subunit protein bL21" }, "genes": [ "rplU" ], "id": "NKIECMJPJD_1163", "locus": "GCA000828735_01160" }, { "type": "cds", "sequence": "contig_1", "start": 670929, "stop": 671051, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTAAAATAAATCATTATCCAAACGCTTTATTAGTAAAAGGTCATGCAAATTTTGATGAGCATGGCAAAGATATAGTATGCGCTGGAGTGAGTGCTATTATTATGGGTGCACTTAATTGA", "aa": "MIKINHYPNALLVKGHANFDEHGKDIVCAGVSAIIMGALN", "aa_hexdigest": "b917f162790c6e7ae7c6b2c53a7b3184", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4275.972199999998, "isoelectric_point": 6.80529842376709 }, "id": "NKIECMJPJD_1164", "locus": "GCA000828735_01161" }, { "type": "cds", "sequence": "contig_1", "start": 672281, "stop": 672445, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATAAAGAGATTTTAATAAGAAATAGTGATTTAAATTATATTGTTAGTGATCCTTTTGAAAACATGATTGGAGGTTTTCCTATTATTTTATTTGAAAATGATAATTATATATATTATTTATCTGGTCAAAATGCTTATTTACCTTCAAAAATGCGTCGTTGA", "aa": "MYKEILIRNSDLNYIVSDPFENMIGGFPIILFENDNYIYYLSGQNAYLPSKMRR", "aa_hexdigest": "1e992452277769364260b2000be80394", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6441.323400000001, "isoelectric_point": 4.992987251281736 }, "id": "NKIECMJPJD_1165", "locus": "GCA000828735_01162" }, { "type": "cds", "sequence": "contig_1", "start": 672464, "stop": 672625, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATCAATAAAAATGACTTATTAAAAGAAAATTTTATTTATAAGGATATTTATATTAATTTATTTCAAATCTATGTTACTAAAAAAAATAATTTAAGCAGATATTTAAGTAACCAGTTTAATTTTACGTTAACAAAAAACGATTTTAAAGACGAATTGTGA", "aa": "MINKNDLLKENFIYKDIYINLFQIYVTKKNNLSRYLSNQFNFTLTKNDFKDEL", "aa_hexdigest": "d206e61713d938c70f3b1a7b8bdecb37", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6527.4128, "isoelectric_point": 9.004250907897951 }, "id": "NKIECMJPJD_1166", "locus": "GCA000828735_01163" }, { "type": "cds", "sequence": "contig_1", "start": 672656, "stop": 672898, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00307DFEB7", "UniRef:UniRef90_UPI00307DFEB7" ], "nt": "TTGATTAATAATTCCAATCCACAAATTTTACTATTTGAAATTTCAAGAAATAAAAAGCAAAAATATCATAGTTTATTACTTTTTGCTGATAAAAATGTATTGAAAAAATTTTATTTAAATTCTGCAACAAAAGATAATTATGAAGATATTATTGATTTGGCCTTTAATAAATTTGAAAATTTACAAAATTTATTAAAAACAACAAAAGTTAAAAACCATTTAAAAAATTTGAAAAAGTATTAA", "aa": "MINNSNPQILLFEISRNKKQKYHSLLLFADKNVLKKFYLNSATKDNYEDIIDLAFNKFENLQNLLKTTKVKNHLKNLKKY", "aa_hexdigest": "206e2e1bd36b2a3628e12227469b9eea", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 672464, "stop": 672898, "observations": { "mutation": [ 672623 ], "stop": [ 672623 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00307DFEB7", "query_cov": 1.0, "subject_cov": 0.5555555555555556, "identity": 0.988, "score": 155.0, "evalue": 3.32e-47, "gene_start": 1, "gene_end": 80, "reference_start": 65, "reference_end": 144, "reference_sequence": "MINKNDLLKENFIYKDIYINLFQIYVTKKNNLSRYLSNQFNFTLTKNDFKDELWNKLIYNTIKKLINNSNPQILLFEISRNKKQKYHSLLLFADKNVLKKFYLNSATKDNYEDIIDLAFNKFENLQNLLKTTKVKNHLKNLKKY" }, "paralog": false, "identity": 0.9861111111111112, "score": 285.0, "evalue": 7.1e-101, "description": "Internal stop codon at 672623. Nonsense mutation around 672623.", "psc": { "uniref90_id": "UniRef90_UPI00307DFEB7", "uniref50_id": "UniRef50_UPI00307DFEB7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00307DFEB7", "UniRef:UniRef50_UPI00307DFEB7" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00307DFEB7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00307DFEB7" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 9561.028599999992, "isoelectric_point": 9.80327205657959 }, "id": "NKIECMJPJD_1167", "locus": "GCA000828735_01164" }, { "type": "cds", "sequence": "contig_1", "start": 673003, "stop": 673269, "strand": "+", "frame": 1, "gene": null, "product": "Asp23/Gls24 family envelope stress response protein", "db_xrefs": [ "RefSeq:WP_006688901.1", "SO:0001217", "UniParc:UPI00000C1BCB", "UniRef:UniRef100_A0AAC9T359", "UniRef:UniRef50_A0A084EXH6", "UniRef:UniRef90_A0AAC9T359" ], "nt": "ATGTTAAAAAAAACTAAATTTGATACTCAACTTGTTAAAAGTTTAATTACATCCTCTATTTTAAAAGTTCCTGGGATTTTTAGTGTTGACATAAATGACAAATTATCAGATTTTAATCGTAATTATTTTGTAATTAAAATTGATTTACATGAAGATGTTGTTAATGTTTTAAGTGTAGCAAATGAAGCGCGTAATTTAGTATATTATGAATTGTCAAAACAATTAAATGATGATAATGTGGTTATTAACATCATTATAAACTGTTAA", "aa": "MLKKTKFDTQLVKSLITSSILKVPGIFSVDINDKLSDFNRNYFVIKIDLHEDVVNVLSVANEARNLVYYELSKQLNDDNVVINIIINC", "aa_hexdigest": "418a95796f58a071b86e6bedc031709a", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI00000C1BCB", "ncbi_nrp_id": "WP_006688901.1", "uniref100_id": "UniRef100_A0AAC9T359", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1BCB", "RefSeq:WP_006688901.1", "UniRef:UniRef100_A0AAC9T359" ] }, "ips": { "uniref100_id": "UniRef100_A0AAC9T359", "uniref90_id": "UniRef90_A0AAC9T359" }, "psc": { "uniref90_id": "UniRef90_A0AAC9T359", "product": "Asp23/Gls24 family envelope stress response protein", "uniref50_id": "UniRef50_A0A084EXH6" }, "pscc": { "uniref50_id": "UniRef50_A0A084EXH6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A084EXH6" ], "product": "Asp23/Gls24 family envelope stress response protein" }, "genes": [], "id": "NKIECMJPJD_1168", "locus": "GCA000828735_01165" }, { "type": "cds", "sequence": "contig_1", "start": 673284, "stop": 674978, "strand": "+", "frame": 3, "gene": "fakA", "product": "DhaL domain-containing protein", "db_xrefs": [ "COG:COG1461", "COG:I", "RefSeq:WP_006688935.1", "SO:0001217", "UniParc:UPI00000C1BCA", "UniRef:UniRef100_A0A2C9DYP5", "UniRef:UniRef50_A0AAC9T074", "UniRef:UniRef90_A0AAC9T074" ], "nt": "ATGAAAACAATTAAGTTAGATCTTTTTCAAAAAATGTTATATGCAGGTGCAAAAATATTAGAAATTGAATGTGAATATATTAACGATTTAAATGTTTTTCCTGTTCCAGATGGTGATACTGGTTCAAACATGAAAACCACAACAGCTGGTGCAATTATTGCTATTAAAGAGATAGAAATATCCAATTTTTCCCAACTTGCTAAAATTTTTAGTCGTGGTCTATTAATGAACGCCAGAGGTAACTCTGGTGTTATTTTAAGTCAAATATTTCGTGGATTTATGGATGCTTTAAATAACGATGCTTATGAAGTTAGTATTGAACAATTAAAACAAGCTTTTAATGGTGCAAAAGAAAAAGCTTATGCAAGCGTCTCTACACCTATTGAAGGAACAATTTTAACAGTCATTCGCGTTATTAATGAGCGAATAAGTGAACGTCAAAATTTTGATAGTGTCGAAGATTTATTTGCATTTGTTACAAAAGAAGCGCAAATCATTCTAGAAAGAACACCAGAAATGTTACCTTCGCTAAAAGAAGCTAATGTTGTTGATTCTGGTGGATATGGTTTAGTAAGTTTTCTAAAAGGAATGTATCAAAGCTTAACAAATACATTTGATGAAAAATTAATTATTAAAAACGAAGAAGAAAATATTAAATCAAAGACTAAACCGCTTGATCTTGAAATTATACATAAAGAAAAAGAAGAAGGTTTTGGTTATTGTAGTGAAATTATTTTATCTTTACGAAAAAAAATTATCCCTAATTCACCAAGTAAAAGTTTTTTTGAATTTGAAAAATATAAAAGTGAATTAGCAAAAATCGGTGATAGTATTGTAGCAATCCAAGATGAAGATCTAGTTAAAGTTCATTTGCATACCCTAACTCCTTATAAATTCTTACAATTATCACAAAAATATGGTGAATTTGATAAGATTAAAATTGAAAACATGACAAACCAATTTTATGAAAGTTTAGAACGAAAAGGGTTGACTACAAAAAAAGAAAATAAATTGACTTTAAGTATGGAGCAAAAAATTATTGCAACAGTTCCATCTGAAAAGTTTAAAAAAATGTTTATTGAAGAACATGGTATTGATAATGTTATTGTTACTGAAACAGAAGGAGCTCCATCAATTCAACGTTTTGTCGATGAAATTAACTCAACAAACTCTTCACAAATTTTTATTGTCACTGATGATTCAAATATTGTATTAGCTGCTGAACAAGCTTCAAAAATCGTTGCTGAAAACAACATTTTGGTTTCTGTAATTGGAGCTTATAATGTTTTTGAAACGCTTTTAGCGATTTCTCATTTTGAACAGCAAAATGATTATCGCACAAATTTTAAAACAATGAATAAAATAGTTAAAAAAGCACGTAGTGGGATGATTACTACTTCAATTAAAAATGTTAAATATCCACATATTACTGTTAATAAAGATGATTATATAGGCATTATTAATAAAAAAATTATCGCTTCTGAAAAAAATGCTTATGATGTTTTATTAAAAACAATTGAAATGTTAATTGAAAGTTTAAAAAAACCAGAAATTTGTTATATATATTATGGCATTAATGCTTCACAAACAGATGTACATCAAATTGAAAAATACATTTCAGAAAAATATGGAATTATTTGTGATTTAAGATATACTGGTCAAAGTTTATATCATTACTACATAGGAATTCAATAA", "aa": "MKTIKLDLFQKMLYAGAKILEIECEYINDLNVFPVPDGDTGSNMKTTTAGAIIAIKEIEISNFSQLAKIFSRGLLMNARGNSGVILSQIFRGFMDALNNDAYEVSIEQLKQAFNGAKEKAYASVSTPIEGTILTVIRVINERISERQNFDSVEDLFAFVTKEAQIILERTPEMLPSLKEANVVDSGGYGLVSFLKGMYQSLTNTFDEKLIIKNEEENIKSKTKPLDLEIIHKEKEEGFGYCSEIILSLRKKIIPNSPSKSFFEFEKYKSELAKIGDSIVAIQDEDLVKVHLHTLTPYKFLQLSQKYGEFDKIKIENMTNQFYESLERKGLTTKKENKLTLSMEQKIIATVPSEKFKKMFIEEHGIDNVIVTETEGAPSIQRFVDEINSTNSSQIFIVTDDSNIVLAAEQASKIVAENNILVSVIGAYNVFETLLAISHFEQQNDYRTNFKTMNKIVKKARSGMITTSIKNVKYPHITVNKDDYIGIINKKIIASEKNAYDVLLKTIEMLIESLKKPEICYIYYGINASQTDVHQIEKYISEKYGIICDLRYTGQSLYHYYIGIQ", "aa_hexdigest": "48d66fed08fcbcf996ad8d4d7146cd31", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI00000C1BCA", "ncbi_nrp_id": "WP_006688935.1", "uniref100_id": "UniRef100_A0A2C9DYP5", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1BCA", "RefSeq:WP_006688935.1", "UniRef:UniRef100_A0A2C9DYP5" ] }, "ips": { "uniref100_id": "UniRef100_A0A2C9DYP5", "uniref90_id": "UniRef90_A0AAC9T074" }, "psc": { "uniref90_id": "UniRef90_A0AAC9T074", "gene": "FakA", "product": "DhaL domain-containing protein", "uniref50_id": "UniRef50_A0AAC9T074", "cog_id": "COG1461", "cog_category": "I" }, "pscc": { "uniref50_id": "UniRef50_A0AAC9T074", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T074" ], "product": "DhaL domain-containing protein" }, "genes": [ "dhaL", "fakA" ], "id": "NKIECMJPJD_1169", "locus": "GCA000828735_01166" }, { "type": "cds", "sequence": "contig_1", "start": 674983, "stop": 675144, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAAAAATTAAAATTTTATTAGATACAATGGGTTATGAAAATGATTTAGAACATGTAATTAAAGCAGCTAAAGATTTTTATTATCAACATGAAGATGATTTAGAAATTATCTTAGTAGGAAATGAACAATTAATTAAGCCATTATTAGATAATGATTGA", "aa": "MEKIKILLDTMGYENDLEHVIKAAKDFYYQHEDDLEIILVGNEQLIKPLLDND", "aa_hexdigest": "b24a1f5275bc1358d222e3ee1f02f4ec", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6248.0486999999985, "isoelectric_point": 4.382252311706543 }, "id": "NKIECMJPJD_1170", "locus": "GCA000828735_01167" }, { "type": "cds", "sequence": "contig_1", "start": 675229, "stop": 675978, "strand": "+", "frame": 1, "gene": "plsX", "product": "phosphate acyltransferase PlsX", "db_xrefs": [ "COG:COG0416", "COG:I", "EC:2.3.1.274", "GO:0005737", "GO:0006633", "GO:0008610", "GO:0008654", "GO:0043811", "KEGG:K03621", "SO:0001217", "UniRef:UniRef50_Q9PQT7", "UniRef:UniRef90_Q9PQT7" ], "nt": "ATGCATTTAGCTTTAAGATATTTAAAAGATAAGCAAGCTGATGGAATGTTAACAGCTGGAAATTCTGCTGTTTTTGTTTATAATGCTTATGCTACAATCGGATTATTAGAACATATTAAGAAACCTGCTTTTATGCCTTTTGTTCCAACAATTGATGGTGGTGTTACAAATTTATTAGATGTGGGTGCATCAATTGATGTTGATGGTACAGATCTATTTAATTTTGCAATCATGGCTAATACAATTGCTAAAATGCGTACTCCAAATCCTCGTGTTGGCGTTTTAAATATTGGTACTGAAGATCATAAAGGTTTACCATATCATCAAGAAGCTAATGAATTATTAAAAACATCAAACTTAAATTATGTGGGTTTTGTAGAACCAAAAACAATTTTAGAACGAGAAGTTGATGTTTTAGTTGCTGATGGTTTTAGTGGAAACATTGCTTTAAAAACAATGGAAGGTGTTGGTAAAACAATTTCTAATTTTTTAAAAAATGAATATAAAAAACCAAAAAATCTTTTTGCTGCTTTATTGTCAAAACCAATTTTTAAAAAAATAAAAAAAGCATTTGATTATAAAGAACATGCTGGAGCTTTTGTTTTAGGATTAGATGGCATTTTAGTTAAAACCCATGGTAGTGCTGATTACCAACAATTTATGAGTGCTTTAAAAATTTTGTATGAAACAATTAAAGCAGATGTTTTAAATGAAATTAAAAAAGATTTAAATAATTATTATGGACAATAA", "aa": "MHLALRYLKDKQADGMLTAGNSAVFVYNAYATIGLLEHIKKPAFMPFVPTIDGGVTNLLDVGASIDVDGTDLFNFAIMANTIAKMRTPNPRVGVLNIGTEDHKGLPYHQEANELLKTSNLNYVGFVEPKTILEREVDVLVADGFSGNIALKTMEGVGKTISNFLKNEYKKPKNLFAALLSKPIFKKIKKAFDYKEHAGAFVLGLDGILVKTHGSADYQQFMSALKILYETIKADVLNEIKKDLNNYYGQ", "aa_hexdigest": "2dff98ced4be68873ec47d6f94cc9f1d", "start_type": "ATG", "rbs_motif": null, "genes": [ "plsX" ], "truncated": "5-prime", "pseudogene": { "start": 674983, "stop": 675978, "observations": { "mutation": [ 675142 ], "stop": [ 675142 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQT7", "query_cov": 1.0, "subject_cov": 0.7522658610271903, "identity": 1.0, "score": 495.0, "evalue": 9.36e-176, "gene_start": 1, "gene_end": 249, "reference_start": 83, "reference_end": 331, "reference_sequence": "MEKIKILLDTMGYENDLEHVIKAAKDFYYQHEDDLEIILVGNEQLIKPLLDNDWRLFPIVHTEVSIEQNDTILSARKKQNSSMHLALRYLKDKQADGMLTAGNSAVFVYNAYATIGLLEHIKKPAFMPFVPTIDGGVTNLLDVGASIDVDGTDLFNFAIMANTIAKMRTPNPRVGVLNIGTEDHKGLPYHQEANELLKTSNLNYVGFVEPKTILEREVDVLVADGFSGNIALKTMEGVGKTISNFLKNEYKKPKNLFAALLSKPIFKKIKKAFDYKEHAGAFVLGLDGILVKTHGSADYQQFMSALKILYETIKADVLNEIKKDLNNYYGQ" }, "paralog": false, "identity": 0.9969788519637462, "score": 653.0, "evalue": 1.67e-240, "description": "Internal stop codon at 675142. Nonsense mutation around 675142.", "psc": { "uniref90_id": "UniRef90_Q9PQT7", "gene": "plsX", "product": "phosphate acyltransferase PlsX", "ec_ids": [ "2.3.1.274" ], "uniref50_id": "UniRef50_Q9PQT7", "cog_id": "COG0416", "cog_category": "I", "kegg_orthology_id": "K03621", "go_ids": [ "GO:0005737", "GO:0006633", "GO:0008610", "GO:0008654", "GO:0043811" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQT7", "EC:2.3.1.274", "UniRef:UniRef50_Q9PQT7", "COG:COG0416", "COG:I", "KEGG:K03621", "GO:0005737", "GO:0006633", "GO:0008610", "GO:0008654", "GO:0043811" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQT7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQT7" ], "product": "Phosphate acyltransferase" } }, "id": "NKIECMJPJD_1171", "locus": "GCA000828735_01168" }, { "type": "cds", "sequence": "contig_1", "start": 675968, "stop": 676570, "strand": "+", "frame": 2, "gene": "rnc", "product": "ribonuclease III", "db_xrefs": [ "COG:COG0571", "COG:K", "EC:3.1.26.3", "GO:0003723", "GO:0003725", "GO:0004525", "GO:0005737", "GO:0006364", "GO:0006396", "GO:0006397", "GO:0008033", "GO:0010468", "GO:0019843", "GO:0046872", "SO:0001217", "UniRef:UniRef50_Q9PQT8", "UniRef:UniRef90_Q9PQT8" ], "nt": "ATGGACAATAAAAAATTTTTAGATTTTTTAAAACAAAATAGAATTGAACCAAAAAATTTATCTATTTATCTTGAAGCATTAACACATAAATCTTATGCAAACGAGCATAAACTTACTAAAAACTATCAACGTTTAGAATTTTTAGGTGATGCTTGTGTTGAATGGGTAATTAGCAATTTTATTTTTAACTACAAAATTAAAGATAATGAAAAAATGCGTTCTTTAGATGAAGGTGAAATGACTCGAGCGCGTTCTAATATGGTACGAAGCGAGATTTTATCATATGCTGCTAAAGATTTAGGGTTAACTGATTTTTTAATGATTGGTGTTGGTTTAGAACAAGACCAATCAGCTCGTATGGAAAAAATCTATGAAGATATTTTTGAAGCATTTATAGGTGCTGTAGCTCAAGATCAAGGTATTAAAAAAGTTTCATTAATTCTTGAAAAAACTTTAATTAAATATTTTAGAGAAGGCCAAATTAATTATCAAAAAGATTATAAAACGATTTTTCAAGAACAAGCCCAACGTATTAATAAAAAACCTATTATGTATAAATTAGTACGTAATGAAGGCGATAAAAAAGAAGTCCATTTAGTTTGA", "aa": "MDNKKFLDFLKQNRIEPKNLSIYLEALTHKSYANEHKLTKNYQRLEFLGDACVEWVISNFIFNYKIKDNEKMRSLDEGEMTRARSNMVRSEILSYAAKDLGLTDFLMIGVGLEQDQSARMEKIYEDIFEAFIGAVAQDQGIKKVSLILEKTLIKYFREGQINYQKDYKTIFQEQAQRINKKPIMYKLVRNEGDKKEVHLV", "aa_hexdigest": "5c05027f71ae074cdec618d997aaae1b", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_Q9PQT8", "query_cov": 1.0, "subject_cov": 0.8368200836820083, "identity": 1.0, "score": 392.0, "evalue": 2.26e-137, "valid": true, "gene": "rnc", "product": "ribonuclease III", "ec_ids": [ "3.1.26.3" ], "uniref50_id": "UniRef50_Q9PQT8", "cog_id": "COG0571", "cog_category": "K", "go_ids": [ "GO:0003723", "GO:0003725", "GO:0004525", "GO:0005737", "GO:0006364", "GO:0006396", "GO:0006397", "GO:0008033", "GO:0010468", "GO:0019843", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQT8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQT8" ], "product": "Ribonuclease 3" }, "genes": [ "rnc" ], "id": "NKIECMJPJD_1172", "locus": "GCA000828735_01169" }, { "type": "cds", "sequence": "contig_1", "start": 676789, "stop": 677058, "strand": "+", "frame": 1, "gene": "rpsO", "product": "30S ribosomal protein S15", "db_xrefs": [ "COG:COG0184", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022627", "RefSeq:WP_004025791.1", "SO:0001217", "UniParc:UPI00000C1BC9", "UniRef:UniRef100_Q9PQT9", "UniRef:UniRef50_P47663", "UniRef:UniRef90_Q9PQT9" ], "nt": "ATGGCAGTAAGCAAACAACAAAAACATGATTTAACAGTTAAATTTGGTGGTTCTGCATCAAATACAGGAAAAACAGAAGTTCAAGTAGCAATCTTAAGTGCTGAAATTGATTCATTAACAACACATATGATTGAAAATAAAAAAGATAAAGCTTCAAAGCGTGGTTTATACAAAAAAGTAGCTCAACGTAAAAAATTATTATCATACTTACAACGTGTTGATATTGAACGTTATCGTGCTTTAATTAAAGAATTAAACTTAAGAGGATAA", "aa": "MAVSKQQKHDLTVKFGGSASNTGKTEVQVAILSAEIDSLTTHMIENKKDKASKRGLYKKVAQRKKLLSYLQRVDIERYRALIKELNLRG", "aa_hexdigest": "9e9d5afee89d48d12579ec11e0742d42", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI00000C1BC9", "ncbi_nrp_id": "WP_004025791.1", "uniref100_id": "UniRef100_Q9PQT9", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1BC9", "RefSeq:WP_004025791.1", "UniRef:UniRef100_Q9PQT9" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQT9", "uniref90_id": "UniRef90_Q9PQT9" }, "psc": { "uniref90_id": "UniRef90_Q9PQT9", "gene": "rpsO", "product": "30S ribosomal protein S15", "uniref50_id": "UniRef50_P47663", "cog_id": "COG0184", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022627" ] }, "pscc": { "uniref50_id": "UniRef50_P47663", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47663" ], "product": "Small ribosomal subunit protein uS15" }, "genes": [ "rpsO" ], "id": "NKIECMJPJD_1173", "locus": "GCA000828735_01170" }, { "type": "cds", "sequence": "contig_1", "start": 677401, "stop": 677532, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAACTAGTGATATTGCTGTTGTATTAAACTCATTAATTTTTAGATTATTAAAAATGCGTCTAGTTAATAAAAAGCAAATTCATAAACTTAATTTAAAAATTATCAATGAGGCTATGTTACATAAATAA", "aa": "MTTSDIAVVLNSLIFRLLKMRLVNKKQIHKLNLKIINEAMLHK", "aa_hexdigest": "f979baabc08d53c27a34423ea7466ae3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5030.161899999999, "isoelectric_point": 10.65547962188721 }, "id": "NKIECMJPJD_1174", "locus": "GCA000828735_01171" }, { "type": "cds", "sequence": "contig_1", "start": 677608, "stop": 678657, "strand": "-", "frame": 3, "gene": null, "product": "Copper-transporting P-type ATPase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQU0", "UniRef:UniRef90_Q9PQU0" ], "nt": "ATGGCCATTCTAGCTTTTTTACTACAAGCTTTTGTACCAAGTATTCAAGAGTTACCAATTGCTTTTTTAAATCTTCATGGATCAAATAATGATTCTAATTTATATGAAAAAGCAGCATATGTAGCAGTTTCTGTTCTAGTTATATCATGTCCATGTGCATTTGGTATTGCTGTGCCTTTAGCAGTTTTAATTGGTGCAGGGCATGGTGCTAAAAGTGGGATTACATTTAACAATAGTAATATTTTTGAAAAGATTAAAAAAGTTAATGCAATTGCTTTTGATAAAACAGGGACATTAACTTATGGAAAATTACAATTAAAACAAGTTATAGGAAATGATCAATTTTTAGATTTAATTTATCAAATGGAATTAATTTCTTTACATCCACTAGCAAAATCATTCGTAACTTATGCCATTATTAATAATATCGTCATGAGTACAAAATTAATTGATATTAAAGAAGTTGCAGGAGTGGGGATTATTGCTAAAGATGTTGATGGTAATATTTATGAATTAACATCAGAACATTATGCAAATGAAAATCAATTTGATTTTTCATTGATTAATCAAAAATCTTCTACTTCTACTAATTTATTAGCTTCAAATATTATTTTTAGTATTAACAAAAAGGTGCAATCAATTCTTGTTTTTGAAGATGAAATTCGTGCTGATGCTTATGAAACAATTAAGGTATTGCATGAAAATAATATTGAAACTTATATGATTACTGGTGATAGTTTTAATGTTGCTCAAAAAATCGCAAAGGAATTAGGAATTAAACATTTTTATGCTCAAGTTAAACCAGAAGAAAAAGCTAACATTATTAAAAAAATTCAAAATGATCAAAAAACTGTAATGTATGTTGGCGATGGTATTAATGATTTATTAGCTTTAAAACAAGCTAATGTTTCTATATCAATTGGTGAAACTAATAAAGCAACTAATGCAGTTGCTGATATTAGTTTAATCAAACCAGATATTTTAAATATTTACAAAGTAATTAAACTAACAAAAATTACTAAGATGTTTATTGTAAGTAGTTTACTATGA", "aa": "MAILAFLLQAFVPSIQELPIAFLNLHGSNNDSNLYEKAAYVAVSVLVISCPCAFGIAVPLAVLIGAGHGAKSGITFNNSNIFEKIKKVNAIAFDKTGTLTYGKLQLKQVIGNDQFLDLIYQMELISLHPLAKSFVTYAIINNIVMSTKLIDIKEVAGVGIIAKDVDGNIYELTSEHYANENQFDFSLINQKSSTSTNLLASNIIFSINKKVQSILVFEDEIRADAYETIKVLHENNIETYMITGDSFNVAQKIAKELGIKHFYAQVKPEEKANIIKKIQNDQKTVMYVGDGINDLLALKQANVSISIGETNKATNAVADISLIKPDILNIYKVIKLTKITKMFIVSSLL", "aa_hexdigest": "ed03ea7e6a22225afac2d9b756592d28", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 677401, "stop": 678957, "observations": { "mutation": [ 677610, 678681 ], "stop": [ 677610, 678681 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQU0", "query_cov": 1.0, "subject_cov": 0.4922425952045134, "identity": 0.968, "score": 630.0, "evalue": 8.44e-222, "gene_start": 1, "gene_end": 349, "reference_start": 292, "reference_end": 640, "reference_sequence": "MLKNNLIKKFQLLPINKRRYLISLMKTSIALLISIPLMVFEMLFMFKSNLILGLDGYFIYGWIVFVLSIFIVFGLGFSFFKGAFFEVFKWKKPGMSLLVVISTCVAFIYSTYSLISNTIIYEPKLHGFFETACMIIATMSVGQLVSDRIKLKANQDLQSLNNLQVKKYNSYDLNTKQVNEKVVFQAKINEYALVKKGEIVPLDGVLYSQIAEVDESSLTGEARPILKTINNDIIAGSINVGDNFIFKITKLYNDSTIKKIINGVNQIASSKPKIQVVADKISLWFTPFILLMAILAFLLQAFVPSIQELPIAFLNLHGSNNDSNLYEKAAYVAVSVLVISCPCAFGIAVPLAVLIGAGHGAKSGITFNNSNIFEKIKKVNAIAFDKTGTLTYGKLQLKQVIGNDQFLDLIYQMESISLHPLAKSFVTYAIINNISMSTKLIDIKEVAGVGIIAKDIDGNVYELTSEHYANENQFDFSLINQKSSTSTNLLASNIIFSINKKVQSILVFEDEIRADAYETIKVLHENNIETYMITGDNTKVAQKIANELGIKHFYAQVKPEEKANIIKEIQNQQKTVMYVGDGINDLLALKQANVSISIGETNKATNAVADISLIKPDILNIYKVIKLTKTTKMFIVSSLLWAFGYNLIFIPLALIGIIPPFISVLIMTTSDIAVVLNSLIFRLLKMRLVNRKQAHKLDLKIIDKAMLHK" }, "paralog": false, "identity": 0.9652509652509652, "score": 952.0, "evalue": 0.0, "description": "Internal stop codon at 677610, 678681. Nonsense mutation around 677610, 678681.", "psc": { "uniref90_id": "UniRef90_Q9PQU0", "product": "Copper-transporting P-type ATPase", "uniref50_id": "UniRef50_Q9PQU0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQU0", "UniRef:UniRef50_Q9PQU0" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQU0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQU0" ], "product": "Copper-transporting P-type ATPase" } }, "id": "NKIECMJPJD_1175", "locus": "GCA000828735_01172" }, { "type": "cds", "sequence": "contig_1", "start": 678679, "stop": 679248, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCTTTACTTGTTGTTATTTCAACATGTGTTGCTTTCATATATAGTACTTATAGTTTAATTTCTAACACAATTATTTATGAACCTAAACTACATGGTTTTTTTGAAACAGCTTGCATGATTATAGCGACAATGAGTGTTGGTCAATTAGTTAGTGATCGTATTAAACTAAAAGCAAATCAAGATCTACAATCTTTAAATAATTTACAAGTCAAAAAATATAATAGTTATGATTTGAACACAAAACAAGTTAGTGAAAAAGTTGTTTTTCAAGCTAAAATTAACGAATACGCACTAGTTAAAAAAGGTGAAATTGTTCCATTAGATGGCGTATTATATTCACAAATTGCTGAAGTTGATGAATCATCATTAACTGGTGAAGCTCGTCCAATTCTAAAAACAATTAATAATGATATTATTGCTGGATCAATTAATGTTGGAGATAATTTTATTTTTAAAATTACTAAACTTTATAATGATTCAACGATTAAAAAAATTATTAATGGTGTTAATCAAATTGCTAGTAGCAAGCCAAAAATTCAAGTTGTTGCTGATAAGATTTCGTTATGA", "aa": "MSLLVVISTCVAFIYSTYSLISNTIIYEPKLHGFFETACMIIATMSVGQLVSDRIKLKANQDLQSLNNLQVKKYNSYDLNTKQVSEKVVFQAKINEYALVKKGEIVPLDGVLYSQIAEVDESSLTGEARPILKTINNDIIAGSINVGDNFIFKITKLYNDSTIKKIINGVNQIASSKPKIQVVADKISL", "aa_hexdigest": "44d68b5de28b285073c08ed3e54bb108", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 20912.074500000017, "isoelectric_point": 8.72890644073486 }, "id": "NKIECMJPJD_1176", "locus": "GCA000828735_01173" }, { "type": "cds", "sequence": "contig_1", "start": 679345, "stop": 679530, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAAAGAATAATTTAATTAAAAAATTTCAATTACTACCTATTAATAAAAGAAGATATTTAATTTCTTTAATGAAAACATCAATAGCACTTCTAATTAGTATTCCTTTAATGGTTTTTGAAATGCTATTTATGTTTAAAAGTAATTTAATTTTAAGCATTGATGGTTATTTTATATATGGATGA", "aa": "MLKNNLIKKFQLLPINKRRYLISLMKTSIALLISIPLMVFEMLFMFKSNLILSIDGYFIYG", "aa_hexdigest": "1f2647e6109bb3b268898b2c4d98e724", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7200.871999999996, "isoelectric_point": 10.092283821105958 }, "id": "NKIECMJPJD_1177", "locus": "GCA000828735_01174" }, { "type": "cds", "sequence": "contig_1", "start": 679534, "stop": 679758, "strand": "-", "frame": 3, "gene": "copZ", "product": "HMA domain-containing protein", "db_xrefs": [ "COG:COG2608", "COG:P", "RefSeq:WP_006688893.1", "SO:0001217", "UniParc:UPI00000C1BC7", "UniRef:UniRef100_A0A2C9DYU1", "UniRef:UniRef50_A0A2C9DYU1", "UniRef:UniRef90_A0A2C9DYU1" ], "nt": "ATGACTTATATGCTATTAACATCTAGTGACTTACATTGCGGGTCATGTTCTTCAAATTTATATAATGTACTTGAAAAGATTGGCGCACAAAATATTAGTGTAAATATTTTAAATTCAGAATTTGCTTTTGAATTTGAAGAAAATAAAATTGCTGATCAAGATGTAATTAAAGAAATCAATAAAAATGGTTTCAAAACAAATATTTTAGAAAAATACATATACTAA", "aa": "MTYMLLTSSDLHCGSCSSNLYNVLEKIGAQNISVNILNSEFAFEFEENKIADQDVIKEINKNGFKTNILEKYIY", "aa_hexdigest": "a4500e4177adfc807110ce24272f44a9", "start_type": "ATG", "rbs_motif": "GGAGG", "ups": { "uniparc_id": "UPI00000C1BC7", "ncbi_nrp_id": "WP_006688893.1", "uniref100_id": "UniRef100_A0A2C9DYU1", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1BC7", "RefSeq:WP_006688893.1", "UniRef:UniRef100_A0A2C9DYU1" ] }, "ips": { "uniref100_id": "UniRef100_A0A2C9DYU1", "uniref90_id": "UniRef90_A0A2C9DYU1" }, "psc": { "uniref90_id": "UniRef90_A0A2C9DYU1", "gene": "CopZ", "product": "HMA domain-containing protein", "uniref50_id": "UniRef50_A0A2C9DYU1", "cog_id": "COG2608", "cog_category": "P" }, "pscc": { "uniref50_id": "UniRef50_A0A2C9DYU1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2C9DYU1" ], "product": "HMA domain-containing protein" }, "genes": [ "copZ" ], "id": "NKIECMJPJD_1178", "locus": "GCA000828735_01175" }, { "type": "cds", "sequence": "contig_1", "start": 679918, "stop": 680088, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAATCAATAAAAATTTAATTCAGTCCTTACATGATCAATCAAAACTATTAGATGAAAACTCAAATTATATTAAATTAATTTTAATGTATCGTCATATTGAACGAAATATTGAACATGGCATAAATATTGTGCAGAACTTTGGTAATGTGCACATTACCAGTCGTTAA", "aa": "MKINKNLIQSLHDQSKLLDENSNYIKLILMYRHIERNIEHGINIVQNFGNVHITSR", "aa_hexdigest": "afe40770043a3e1dbdaf8ea5a0d6beac", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6657.597899999999, "isoelectric_point": 9.301514625549316 }, "id": "NKIECMJPJD_1179", "locus": "GCA000828735_01176" }, { "type": "cds", "sequence": "contig_1", "start": 680362, "stop": 680574, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTACTAATTATTTTTTAATGAAAGAATCACAATGTGATTTACTAAATGATTTTAAAAACTTTTTTCAAATGGTAATTGATAATCAAAAACTAATTTGTGATTTATTAAAAAACGATCAATCATCATATAAAGAAACATATGAAAAAGCTTGTTTTTTAGAAAAAAAAGTTAATACAACATATGCTGATATGTTAGAAGAAATTATGTGA", "aa": "MATNYFLMKESQCDLLNDFKNFFQMVIDNQKLICDLLKNDQSSYKETYEKACFLEKKVNTTYADMLEEIM", "aa_hexdigest": "6c9b76b002d7ada9d9356b4732169967", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8381.5818, "isoelectric_point": 4.580506706237793 }, "id": "NKIECMJPJD_1180", "locus": "GCA000828735_01177" }, { "type": "cds", "sequence": "contig_1", "start": 680685, "stop": 680843, "strand": "-", "frame": 1, "gene": null, "product": "Phosphate ABC transporter ATP-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P9H9", "UniRef:UniRef90_A0A3B0P9H9" ], "nt": "ATGGATGAACCAACAAGTGCTTTAGATCCAATTGCAACAGCTAAAATTGAAGAATTAATATTAGAATTAAAAAAGAAATATTCTATTATTATTGTGACTCACTCAATGGCACAAGCCCAGCGAATAAGTGATGAAACTGTGTTTTTTTATCAAGGGTGA", "aa": "MDEPTSALDPIATAKIEELILELKKKYSIIIVTHSMAQAQRISDETVFFYQG", "aa_hexdigest": "bb12a1e6150c232dc8e11a55bf53d87b", "start_type": "ATG", "rbs_motif": "AGxAG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 680598, "stop": 680927, "observations": { "mutation": [ 680687 ], "stop": [ 680687 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A3B0P9H9", "query_cov": 1.0, "subject_cov": 0.46846846846846846, "identity": 0.846, "score": 90.9, "evalue": 1.43e-22, "gene_start": 1, "gene_end": 52, "reference_start": 31, "reference_end": 82, "reference_sequence": "MFQSAHGLSEGQQQRLSIARAIALKPEVLLMDEPTSALDPIATNKIEQLIHQLKKSYSIVIVTHSMAQAQRVSDETVFFYEGRVLEHGTTKQIFTQPNNEKTKDYIDGRIG" }, "paralog": false, "identity": 0.7339449541284404, "score": 159.0, "evalue": 5.15e-52, "description": "Internal stop codon at 680687. Nonsense mutation around 680687.", "psc": { "uniref90_id": "UniRef90_A0A3B0P9H9", "product": "Phosphate ABC transporter ATP-binding protein", "uniref50_id": "UniRef50_A0A3B0P9H9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A3B0P9H9", "UniRef:UniRef50_A0A3B0P9H9" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3B0P9H9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P9H9" ], "product": "Phosphate ABC transporter ATP-binding protein" } }, "id": "NKIECMJPJD_1181", "locus": "GCA000828735_01178" }, { "type": "cds", "sequence": "contig_1", "start": 680952, "stop": 681317, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00005.33" ], "nt": "ATGAATCGAACAAAACATGTTCTACATGATTTAAATTTGGATATTAAAAGAAATAAAGTAACAGCATTTATTGGCCCAAGTGGTTGTGGTAAATCAACTTTTTTAAGAAATTTAAATCAATTAAACGATTTAATAGAAGGGACTAGTCACGAAGGTGAAATTTATTTTTTAGGAACTAATACACGTTCAAAAAAAATATCATCATTAGAATTAAGAACACGAATCGGGATGGTTTTTCAAAAACCAACACCATTTGAAATGAGTATTTTTGATAATGTTGCATATGGTCCACGAAATAATGGAATTAATGATCGTAAAATTTTAGAAAAAATTGTTGAAAAATCATTAAAAAGTGCAGCTTTATGA", "aa": "MNRTKHVLHDLNLDIKRNKVTAFIGPSGCGKSTFLRNLNQLNDLIEGTSHEGEIYFLGTNTRSKKISSLELRTRIGMVFQKPTPFEMSIFDNVAYGPRNNGINDRKILEKIVEKSLKSAAL", "aa_hexdigest": "d46ba8bed11d8ab3330a7fa4ed6f5a9a", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_W5SUP8", "query_cov": 0.8347107438016529, "subject_cov": 0.8145161290322581, "identity": 0.5539999999999999, "score": 125.0, "evalue": 6.9e-35, "valid": false, "product": "Phosphate transport ATP-binding protein pstB", "uniref50_id": "UniRef50_A0A3D0XTF9" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00005.33", "name": "ABC_tran", "length": 104, "aa_cov": 0.8512396694214877, "hmm_cov": 0.8365384615384616, "evalue": 6.619570536692063e-16, "score": 54.73188018798828, "start": 8, "stop": 110 } ], "seq_stats": { "molecular_weight": 13662.59699999998, "isoelectric_point": 9.807913780212402 }, "id": "NKIECMJPJD_1182", "locus": "GCA000828735_01179" }, { "type": "cds", "sequence": "contig_1", "start": 681450, "stop": 681587, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGATCAAAAAGACAATAAAGAAATTCAAACAATTGTTCCTTTAAATCAAAAAACTAATTTAAATGAATTGGATGCTAAAGCAATTTATGATGAATTTCAAAAATTAAATAAATACCAAAGAGCTTTTGCTTGA", "aa": "MNDQKDNKEIQTIVPLNQKTNLNELDAKAIYDEFQKLNKYQRAFA", "aa_hexdigest": "4a971e1008b84b7909edc270e7f42b59", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5342.946800000001, "isoelectric_point": 6.080771827697753 }, "id": "NKIECMJPJD_1183", "locus": "GCA000828735_01180" }, { "type": "cds", "sequence": "contig_1", "start": 681592, "stop": 681780, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCGTGATATTAAAGTTTTCAAACCACAAATTTATCAAAAACATCTTTATTTAACACATAATCAAGCCAAAATCATGGGATATAATGAATCAGTAAATAAAGTTGCTAAAATTGGTTTAAAGTTATATGAAATTCGTTATGTTGATGATGAACAAATTCAAAAAGAGTTATTAAAAGTTAGGGGATAA", "aa": "MRDIKVFKPQIYQKHLYLTHNQAKIMGYNESVNKVAKIGLKLYEIRYVDDEQIQKELLKVRG", "aa_hexdigest": "041b07d31ae47ce8c2aff510268ed6ab", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7436.6578, "isoelectric_point": 9.600712394714353 }, "id": "NKIECMJPJD_1184", "locus": "GCA000828735_01181" }, { "type": "cds", "sequence": "contig_1", "start": 681895, "stop": 682047, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTATTTAACAGCAGGTTTATCATCAGCTCAAAGCATAACAATTTTAAATCCTGGACAAACATTAACAACACGTATTTATGCTCAATTATATGAAAATAATACAAATATTGCTCATAATGTAATGTACGAATCTGCTTTTATTACTTTATGA", "aa": "MYLTAGLSSAQSITILNPGQTLTTRIYAQLYENNTNIAHNVMYESAFITL", "aa_hexdigest": "20767dfc4da79e5adee472ed540fc434", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5553.235900000001, "isoelectric_point": 5.380629158020019 }, "id": "NKIECMJPJD_1185", "locus": "GCA000828735_01182" }, { "type": "cds", "sequence": "contig_1", "start": 682144, "stop": 682338, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTGGTTTAGTTATTTTTATTGAAATTTTTGGTTTTACTTCAATGGGTAATATTGGTAAATCACTATTAGCGGGTGCTTTAACAATTACTTTAGTAGTCCTTCCAACATTTACACGTTCAATTCAACAATCTTTAAAAGCAGTACCAATGAGCATTCGTGAAAATGCTTATGGATTGGGATGTTCAAAGTGA", "aa": "MFGLVIFIEIFGFTSMGNIGKSLLAGALTITLVVLPTFTRSIQQSLKAVPMSIRENAYGLGCSK", "aa_hexdigest": "ec36543579dc0d99cd07be4f21e8c612", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6853.140700000001, "isoelectric_point": 9.624630165100097 }, "id": "NKIECMJPJD_1186", "locus": "GCA000828735_01183" }, { "type": "cds", "sequence": "contig_1", "start": 682837, "stop": 683526, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTTGTTTCGTTTGGTGCAATTATAATTGCTGCTCCAATTGGTATTAAAACAGCTACTTTTATTAAGTTTAGAATTAAAAACAAAAGATTACAAAAATTTTTTAGAGTCACGATTTTAGCATTAGCTGGAATTCCTTCAGTTATTTTTGGATTATTTGCTATTCAGTCTTTAGGTCCTGCAATTTCGTTTGTTTTCCACATTGATACTGTTCAAAACATTACTACGTCAATGTTTATGTTAGCGTTTATTATTATTCCGACAATTATTTCTTTAACGCTAAGCACATATGATGGTATTGATATGCGTTTAATTGAAAATGGGATAGGAATGGGAAGTTCTTATACACGTTCAATTTATAAGATTTTTAAAAAAGAAGCTCGTGGTGGTATCATTATTGCCATTATTATTGCCTTAGGTCGTGCGATCGGCGAAACAATGGCCATTAGTATGATTTTAAGTGATCAAGGTTACCAAAACATTTTTGGTACTGGTTTTTTACAAATTATGCACTCTGCTCTTCGTCCATTAGGTGCTGTAATTTCAGCTAACATGTTCGCTGAAAATGGTGGCGAAGGTTTAAGAGGATTACTATATATTTATGGGATCGTTTTATTTGTAGCGATTATGATTTTAAATGGCCTTGTAACTTATTTAACAAGAAAACGTTCAAAGAAAACATATGCTTGA", "aa": "MLVSFGAIIIAAPIGIKTATFIKFRIKNKRLQKFFRVTILALAGIPSVIFGLFAIQSLGPAISFVFHIDTVQNITTSMFMLAFIIIPTIISLTLSTYDGIDMRLIENGIGMGSSYTRSIYKIFKKEARGGIIIAIIIALGRAIGETMAISMILSDQGYQNIFGTGFLQIMHSALRPLGAVISANMFAENGGEGLRGLLYIYGIVLFVAIMILNGLVTYLTRKRSKKTYA", "aa_hexdigest": "3def5d03ad42d4fa41c2149739a468e6", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 25015.7534, "isoelectric_point": 10.216707801818849 }, "id": "NKIECMJPJD_1187", "locus": "GCA000828735_01184" }, { "type": "cds", "sequence": "contig_1", "start": 683686, "stop": 683787, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTATTTTAAATCTTAATTCGCAACAGACTAAAAAAATCATTTTTAACAAAAAAATAAAAGATAAACTAGCTAAAACGTTAGTTATTAGTTTTGCTTGA", "aa": "MSILNLNSQQTKKIIFNKKIKDKLAKTLVISFA", "aa_hexdigest": "7d61dad708405164e15fa68a980e06ab", "start_type": "ATG", "rbs_motif": "AGGAG(G)/GGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3777.563500000001, "isoelectric_point": 10.398057365417479 }, "id": "NKIECMJPJD_1188", "locus": "GCA000828735_01185" }, { "type": "cds", "sequence": "contig_1", "start": 683787, "stop": 683927, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTCTTAAAACCTTATAAAGATGAACAATCTTTTCAAGAAGCCATTAACAATCAAAGCGCAAAAAACAATTTTTTTGTAAGTGATTTTGAATTAATAGAATCAAATAGAATTAATATATTAGGAGCAAAATCAGCATAA", "aa": "MFLKPYKDEQSFQEAINNQSAKNNFFVSDFELIESNRINILGAKSA", "aa_hexdigest": "dbba823af5d457ef2eed85d72fcc22d0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5297.861199999998, "isoelectric_point": 5.052213478088378 }, "id": "NKIECMJPJD_1189", "locus": "GCA000828735_01186" }, { "type": "cds", "sequence": "contig_1", "start": 684276, "stop": 684533, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTAAAGCTTTTACTGGGAATGAACAAGTAAGTTATTATGATCTTGATCCCCAATTACCAAAAACACCAATTTTAGCTTATTCACGCACAGGTGGAGCAAATGCCTCAGGGACAGCCGAGGCTTTTTTGACTTCTAATCCATTAATTTCTAAAAAAGAATTTAAAAAAAAATATCATGATATTTATAAGGTTATTGAAGCTGGTACTTATGGCAAATTAATAAGAAGTACTGAAGAATCGAATACTCAAGCTTGA", "aa": "MFKAFTGNEQVSYYDLDPQLPKTPILAYSRTGGANASGTAEAFLTSNPLISKKEFKKKYHDIYKVIEAGTYGKLIRSTEESNTQA", "aa_hexdigest": "a95692d4c103bc4f75be903ac5a6c0de", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9441.510999999995, "isoelectric_point": 8.860228538513184 }, "id": "NKIECMJPJD_1190", "locus": "GCA000828735_01187" }, { "type": "cds", "sequence": "contig_1", "start": 684645, "stop": 684959, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGGTTAAGATATAAAATCTTATGCGCTAGCTTAGGAGTGGTTGGTGTTGTTGGTGTTATTGCTGCAATATCAATTGATTATAAACAAATTCCAATTGTTTATGCAAATGGTAGTTCATCAGTATTACCCTTAATTCAGGCCTTATCTGAAAAACATCATAAACAAGATATTATTTCTAATGCTGGAGGTTCTTCGTTAGGAATAATTGAAGCTGTTGAACAAAGAACTAATATAGGCGTAAGTTCACGTAGTCCAAATATTGATTTTGAGCAACAAAGTGTTGAAAAAAGTCATGTAGTTTATGATAAGTGA", "aa": "MRLRYKILCASLGVVGVVGVIAAISIDYKQIPIVYANGSSSVLPLIQALSEKHHKQDIISNAGGSSLGIIEAVEQRTNIGVSSRSPNIDFEQQSVEKSHVVYDK", "aa_hexdigest": "f9076279e0ff035578fbe8e6290d0773", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11206.731899999995, "isoelectric_point": 7.895588111877441 }, "id": "NKIECMJPJD_1191", "locus": "GCA000828735_01188" }, { "type": "cds", "sequence": "contig_1", "start": 685296, "stop": 685694, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00133.28" ], "nt": "ATGTCTAAATCATTAGGCAATGTAATTGATCCTAATGTTTTAATTGATACTTATGGTGTGGATGCACTTCGTTATTATTTAATGGCTGATTTAAGTTTATTTAGAGATGCTATTTTTAGTGAAGATAATTTGATTGAAACTTATAATACTCAATTAGCAAATAGTTATGGTAACATGATTTCAAGAACATTGGGAATGTTAAAAAAATATCGAAATAATATAGTTCCAAAATATGTTGGTTGTGTTTTAAAAAATGATGAAAAATTAGAAAATTTAATTAATAAAAATATTGAATTAGTTCAAGAAAACATTAATAAATATAGTATTGATAAAGCACTTAATTGCATTCAAGAAATTTTAGTTGAAGCAAATAAATATATTGAAGATAATAAACCTTGA", "aa": "MSKSLGNVIDPNVLIDTYGVDALRYYLMADLSLFRDAIFSEDNLIETYNTQLANSYGNMISRTLGMLKKYRNNIVPKYVGCVLKNDEKLENLINKNIELVQENINKYSIDKALNCIQEILVEANKYIEDNKP", "aa_hexdigest": "8c15c9164e6bc193a5a15b6708c78d11", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00133.28", "name": "tRNA-synt_1", "length": 33, "aa_cov": 0.25, "hmm_cov": 0.9696969696969697, "evalue": 2.224969150627899e-09, "score": 32.06669616699219, "start": 1, "stop": 33 } ], "seq_stats": { "molecular_weight": 15160.218599999986, "isoelectric_point": 4.984063529968262 }, "id": "NKIECMJPJD_1192", "locus": "GCA000828735_01189" }, { "type": "cds", "sequence": "contig_1", "start": 685965, "stop": 686315, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00133.28" ], "nt": "ATGGATCATGAAGAGTCTGTACAAAAAGTTTTTAGTTATTTATATAAACAAGGAAAAATTTATTTAGGCCAATGGACAGGTTATTATTGTGTATCATGCGAAGAAAATTATAATCCTGCTGAAATTATTAAAAGTCAAGATAATATAATGTTATGTCGCATGGGTCATAAATTAGAAACTAAGTCTGAAGAATCATATTTTTATAAAATGAGTGATCAAGCACCATTTTTAAAAACTTACTATCAAAATCATCCGAATTTCATTATTCCAAATGAACGTGCAAATGAAATGGTTAATAACTTCTTAAATAATTTAGAAGATTTAAGTATTTCACGTACTACTTTTGATTGA", "aa": "MDHEESVQKVFSYLYKQGKIYLGQWTGYYCVSCEENYNPAEIIKSQDNIMLCRMGHKLETKSEESYFYKMSDQAPFLKTYYQNHPNFIIPNERANEMVNNFLNNLEDLSISRTTFD", "aa_hexdigest": "cf0f62c02569976ad725240e028e08be", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00133.28", "name": "tRNA-synt_1", "length": 111, "aa_cov": 0.9568965517241379, "hmm_cov": 0.8828828828828829, "evalue": 8.940601866025615e-08, "score": 26.783100128173828, "start": 3, "stop": 113 } ], "seq_stats": { "molecular_weight": 13822.359799999984, "isoelectric_point": 5.149521827697753 }, "id": "NKIECMJPJD_1193", "locus": "GCA000828735_01190" }, { "type": "cds", "sequence": "contig_1", "start": 686361, "stop": 686612, "strand": "-", "frame": 1, "gene": null, "product": "Methionyl/Leucyl tRNA synthetase domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A093YS71" ], "nt": "ATGTTAAAACAAAAGAAATTTTTTATCTCTACTCCCATTTACTATTCTTCTGGAAATCCTCATATTGGTCATGCTTATACAACGATCATTGCTGATGTTTTAGCACGTTATAAACGCTTGTTTGGATATGATGTTTTCTTTTTAACAGGAATGGATGAGCATGGTCAAAAAATCCAACAAAAAGCTTTTGAAGAAAACATTTCTCCCAAAGCTTTGGTCGATCGTAATTCAATTATTTTTTTAAATTTATGA", "aa": "MLKQKKFFISTPIYYSSGNPHIGHAYTTIIADVLARYKRLFGYDVFFLTGMDEHGQKIQQKAFEENISPKALVDRNSIIFLNL", "aa_hexdigest": "61308767792bdd436ce036f9541369a5", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_A0A093YS71", "query_cov": 0.927710843373494, "subject_cov": 0.9871794871794872, "identity": 0.623, "score": 103.0, "evalue": 1.37e-27, "valid": false, "product": "Methionyl/Leucyl tRNA synthetase domain-containing protein", "uniref50_id": "UniRef50_A0A093YS71" }, "pscc": { "uniref50_id": "UniRef50_A0A093YS71", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A093YS71" ], "product": "Methionyl/Leucyl tRNA synthetase domain-containing protein" }, "genes": [], "id": "NKIECMJPJD_1194", "locus": "GCA000828735_01191" }, { "type": "cds", "sequence": "contig_1", "start": 686632, "stop": 687723, "strand": "-", "frame": 3, "gene": "rarA", "product": "AAA family ATPase", "db_xrefs": [ "COG:COG2256", "COG:L", "KEGG:K07478", "SO:0001217", "UniRef:UniRef50_A0A084EZ83", "UniRef:UniRef90_A0AAP9A9S8" ], "nt": "ATGGCCCCCCCAGGTGTAGGAAAATCATCGCTTGCTAAAGTTTTAGCACAAGATTTAAAAGTTCCATTTGCTTTTTTCAATCCAGTTGTTGATTCAAAAAAGCAATTAATGCAAATCATTGAACAAGCATTAGATTTAAATAACTTTATTATCATTATTGATGAAATTCATCGATTAAATAAAGATAAACAAGATATTTTATTACCAATTATTGAAGCAAATAAAATTAAATTATTTGCGACAACAACTGAAAATCCGTTTTTTGTTATTAACCCTGCTTTAAGAAGTCGTTGTCAACTATTATCATTAAAACCATTAACGAGTTCACAAATTAGTGAACGAATTAAAACTTTGATTAATGTTGAAGAAGTTTTTGAACCACAAGCTTTTAATTTATTAATTAATAAAACTAATGGAGATTTAAGGGCTGTTATCAATACATTAGAAATTATTTATTTATTATATAAAGAACATAAAGTTGATTATGTATTACTAAAACGTATTATGCAAGAATCATACGCATTAGGTAGTAGCGATGGCGATGAAATTCATGACTTAAAATCAGCTTTTCATAAATCAATGCGTGGAAGTGATTGTGATGCAGCTTTATATTATTTAGCACGTTTAATAATCATAGGTGATTTTGAAGCGATTTATCGAAGAGTTAGTGTTTGTGTTCATGAAGATATAGGTTTAGCTAATCCTAATCTATGTTTACGTGTTGATCAAGGAATTAATTCTTGTCGATTTTTAGGATATCCAGAAAATCAAGTAATTTTAGCTAATTTAGTTTTACAAATTTGTTTATCAAATAAATCAAACTCTGTATATCTTGCCTATCAAAAAGCATTTGAAGATGCTGCTAATGGCAAACAATATTCAATTCCAAATCATGTCCGTGATGCTCATTATGCATCAGCAATAAAATTGGGTGTTCATGGTTATAAATATCCACACGATTTTAAAAATAACTATGTTAATCAACAATATTTACCACGTGAATTAACAAACAAAAAATATTATGTTCCCCAAAACAACGAACAAGAGTTTAAATTAAACCAAAATCAACAAAAATTTAAAAATAATAATTAA", "aa": "MAPPGVGKSSLAKVLAQDLKVPFAFFNPVVDSKKQLMQIIEQALDLNNFIIIIDEIHRLNKDKQDILLPIIEANKIKLFATTTENPFFVINPALRSRCQLLSLKPLTSSQISERIKTLINVEEVFEPQAFNLLINKTNGDLRAVINTLEIIYLLYKEHKVDYVLLKRIMQESYALGSSDGDEIHDLKSAFHKSMRGSDCDAALYYLARLIIIGDFEAIYRRVSVCVHEDIGLANPNLCLRVDQGINSCRFLGYPENQVILANLVLQICLSNKSNSVYLAYQKAFEDAANGKQYSIPNHVRDAHYASAIKLGVHGYKYPHDFKNNYVNQQYLPRELTNKKYYVPQNNEQEFKLNQNQQKFKNNN", "aa_hexdigest": "81485175de6c8c2447b54f3c24b8d399", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0AAP9A9S8", "query_cov": 0.9944903581267218, "subject_cov": 0.8848039215686274, "identity": 0.978, "score": 694.0, "evalue": 3.88e-251, "valid": true, "gene": "RarA", "product": "AAA family ATPase", "uniref50_id": "UniRef50_A0A084EZ83", "cog_id": "COG2256", "cog_category": "L", "kegg_orthology_id": "K07478" }, "pscc": { "uniref50_id": "UniRef50_A0A084EZ83", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A084EZ83" ], "product": "ATPase, AAA family" }, "genes": [ "rarA" ], "id": "NKIECMJPJD_1195", "locus": "GCA000828735_01192" }, { "type": "cds", "sequence": "contig_1", "start": 687686, "stop": 687859, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAGATAAATTAATTAAAACTCTAAAAAATCCTCATAGTATTGATGACATTGTTGGTCAAAAATATTTATTAGATGAAATTGGTATAATTAGAAGAATGGTTAATCACCATCAAGTATTTAATTTAATTTTTTATGGCCCCCCCAGGTGTAGGAAAATCATCGCTTGCTAA", "aa": "MLDKLIKTLKNPHSIDDIVGQKYLLDEIGIIRRMVNHHQVFNLIFYGPPRCRKIIAC", "aa_hexdigest": "eae69e126ce714eb81288385c4b95bf0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6688.9547, "isoelectric_point": 9.444054222106931 }, "id": "NKIECMJPJD_1196", "locus": "GCA000828735_01193" }, { "type": "cds", "sequence": "contig_1", "start": 687861, "stop": 687962, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAACAAAATTTTAAATTAAAAGATGCTTGTAAAGAAGTTAGTAAATCTTCTAATTTTGATAGTAAAGAATTATATAAATTAATAATTAATCAAAAATAA", "aa": "MKQNFKLKDACKEVSKSSNFDSKELYKLIINQK", "aa_hexdigest": "32f709181d5371fca3b78b43c2138d5b", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3906.5285000000013, "isoelectric_point": 9.519095420837402 }, "id": "NKIECMJPJD_1197", "locus": "GCA000828735_01194" }, { "type": "cds", "sequence": "contig_1", "start": 688152, "stop": 688700, "strand": "-", "frame": 1, "gene": "rsmI", "product": "16S rRNA (cytidine(1402)-2'-O)-methyltransferase", "db_xrefs": [ "COG:COG0313", "COG:J", "EC:2.1.1.198", "GO:0000451", "GO:0008649", "GO:0008757", "KEGG:K07056", "SO:0001217", "UniRef:UniRef50_A0A084EZ84", "UniRef:UniRef90_Q9PQU8" ], "nt": "ATGAAATTATGTGATAAAGAATATATTTTATCTGTTATTGCTACACCAATTGGCAATCTTGAAGAAGTTAGTGAACGTGTTATTAAATGTTTAAATGAAGCTTATGTTATTTTATGTGAAGACACTCGAGTTACAGCTAAGCTGTTGCATCTATTAAATATTTATGATTACAAAAAATTAATTAGTTATCATAATTTTAATGAAATTCAAAAATTGAATGAAGCGATTAATTATATTAAAAAATATCCAACAGCTTTAGTTAGTGATGCAGGCTATCCAACAATTTCAGATCCTGGTTATAAATTAATTAATGAATGTCATCAACAAAATATTGGAATTCAAGTTATTAATGGTCCCTCAAGTTTGATGCATGCTCTAGTTGCTAGTGGAATGTGTTCGCAAGATTTTATGTTTTTAGGTTTTTTAGGTAAAACGCAAAAACAACGTGTTGAAAAACTAAAATTATATAAAAACTTGCAAACAACTTTTGTTATTTATGAAGCTGTGCACCGAATAGAAAAGACTTTAATGGATATATATGAAATATGA", "aa": "MKLCDKEYILSVIATPIGNLEEVSERVIKCLNEAYVILCEDTRVTAKLLHLLNIYDYKKLISYHNFNEIQKLNEAINYIKKYPTALVSDAGYPTISDPGYKLINECHQQNIGIQVINGPSSLMHALVASGMCSQDFMFLGFLGKTQKQRVEKLKLYKNLQTTFVIYEAVHRIEKTLMDIYEI", "aa_hexdigest": "e0b2b8e719ec8b854b9a7328b16e7baf", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [ "rsmI" ], "truncated": "5-prime", "pseudogene": { "start": 687861, "stop": 688700, "observations": { "mutation": [ 688154 ], "stop": [ 688154 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQU8", "query_cov": 1.0, "subject_cov": 0.6523297491039427, "identity": 1.0, "score": 365.0, "evalue": 1.44e-126, "gene_start": 1, "gene_end": 182, "reference_start": 1, "reference_end": 182, "reference_sequence": "MKLCDKEYILSVIATPIGNLEEVSERVIKCLNEAYVILCEDTRVTAKLLHLLNIYDYKKLISYHNFNEIQKLNEAINYIKKYPTALVSDAGYPTISDPGYKLINECHQQNIGIQVINGPSSLMHALVASGMCSQDFMFLGFLGKTQKQRVEKLKLYKNLQTTFVIYEAVHRIEKTLMDIYEIWGDILIFIGRELTKKNESHYYGYLSQLPPITEKGEFVIVIDNKINQDVIKNNNINECLIKIRDLMKQNFKLKDACKEVSKSSNFDSKELYKLIINQK" }, "paralog": false, "identity": 0.996415770609319, "score": 503.0, "evalue": 4.39e-183, "description": "Internal stop codon at 688154. Nonsense mutation around 688154.", "psc": { "uniref90_id": "UniRef90_Q9PQU8", "gene": "rsmI", "product": "16S rRNA (cytidine(1402)-2'-O)-methyltransferase", "ec_ids": [ "2.1.1.198" ], "uniref50_id": "UniRef50_A0A084EZ84", "cog_id": "COG0313", "cog_category": "J", "kegg_orthology_id": "K07056", "go_ids": [ "GO:0000451", "GO:0008649", "GO:0008757" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQU8", "EC:2.1.1.198", "UniRef:UniRef50_A0A084EZ84", "COG:COG0313", "COG:J", "KEGG:K07056", "GO:0000451", "GO:0008649", "GO:0008757" ] }, "pscc": { "uniref50_id": "UniRef50_A0A084EZ84", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A084EZ84" ], "product": "Ribosomal RNA small subunit methyltransferase I" } }, "id": "NKIECMJPJD_1198", "locus": "GCA000828735_01195" }, { "type": "cds", "sequence": "contig_1", "start": 688863, "stop": 689243, "strand": "-", "frame": 1, "gene": "rnmV", "product": "ribonuclease M5", "db_xrefs": [ "COG:COG1658", "COG:J", "EC:3.1.26.8", "GO:0006364", "GO:0043822", "KEGG:K05985", "SO:0001217", "UniRef:UniRef50_Q9PQU9", "UniRef:UniRef90_Q9PQU9" ], "nt": "ATGAATAAACCAATTATCAAAGAAGTTATTATTGTAGAAGGTAAAACAGATGCACAAAAAATTGATCAATTAGTAAATGCACAAATTATTACTACTAATGGATCAGAAATTAATAAAAAGACTTTAGCTTTAATTAAACAAGCACAATTATCAAAAGGAATTATTTTATTTTTAGATCCTGATTATCAAGGAGAAAGAATTCGTAAAATAATTACTAATTATTTAAAAGATGGCATTATTAAACAGTGTTTTATTAGTAAGGACAATATGAAGAATAATTCTAAAAAAATTGGAATTGCTGAAGCTAATAATGATGCTTTATTGAAAGCTTTGAAATCTCAAGGAACATTCGTTATTAATTCAATTGAATCTATTACTTGA", "aa": "MNKPIIKEVIIVEGKTDAQKIDQLVNAQIITTNGSEINKKTLALIKQAQLSKGIILFLDPDYQGERIRKIITNYLKDGIIKQCFISKDNMKNNSKKIGIAEANNDALLKALKSQGTFVINSIESIT", "aa_hexdigest": "26d87bab429e1d78bb34de80c047b42a", "start_type": "ATG", "rbs_motif": null, "genes": [ "rnmV" ], "truncated": "5-prime", "pseudogene": { "start": 688704, "stop": 689243, "observations": { "mutation": [ 688865 ], "stop": [ 688865 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQU9", "query_cov": 1.0, "subject_cov": 0.7039106145251397, "identity": 0.992, "score": 236.0, "evalue": 7.12e-78, "gene_start": 1, "gene_end": 126, "reference_start": 1, "reference_end": 126, "reference_sequence": "MNKPIIKEVIIVEGKTDAQKIDQLVNAQIITTNGSEINKKTLALIKQAQLSKGIILFLDPDYQGERIRKIITNYLKDGIIKQCFISKDNMKNNSKKIGIAEANNDALLKALKSQGTFVINPIESITWLEYLSLNLNNKKIRLMLCDYLNISYCNHKQLFKRLNMMQKTFKEIKIIIEGF" }, "paralog": false, "identity": 0.9888268156424581, "score": 343.0, "evalue": 9.99e-123, "description": "Internal stop codon at 688865. Nonsense mutation around 688865.", "psc": { "uniref90_id": "UniRef90_Q9PQU9", "gene": "rnmV", "product": "ribonuclease M5", "ec_ids": [ "3.1.26.8" ], "uniref50_id": "UniRef50_Q9PQU9", "cog_id": "COG1658", "cog_category": "J", "kegg_orthology_id": "K05985", "go_ids": [ "GO:0006364", "GO:0043822" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQU9", "EC:3.1.26.8", "UniRef:UniRef50_Q9PQU9", "COG:COG1658", "COG:J", "KEGG:K05985", "GO:0006364", "GO:0043822" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQU9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQU9" ], "product": "Ribonuclease M5" } }, "id": "NKIECMJPJD_1199", "locus": "GCA000828735_01196" }, { "type": "cds", "sequence": "contig_1", "start": 689236, "stop": 689778, "strand": "-", "frame": 3, "gene": "prs", "product": "Ribose-phosphate pyrophosphokinase", "db_xrefs": [ "COG:COG0462", "COG:F", "EC:2.7.6.1", "GO:0000287", "GO:0002189", "GO:0004749", "GO:0005524", "GO:0005737", "GO:0006015", "GO:0006164", "GO:0009156", "GO:0016301", "KEGG:K00948", "SO:0001217", "UniRef:UniRef50_Q9PQV0", "UniRef:UniRef90_Q9PQV0" ], "nt": "ATGAAACATTATTTTAGTGCTTATAAAGATTCATCAAATATAACTATTGTTTCACCAGATTATGGTGGTGTCAAACGTGCTCGTGAAATTTCAATAGCTACAGGTGCAACATTAGCAATTGTTGATAAACGAAGATCTGGCAAAAATCAGGTGGAAATTAATAATGTATTAGGTGATGTTAAAGATCGTGATTGTGTAATTGTTGATGATATGATTGATACTGGTGGCACAATTTTAGGTGCTGCTAAAATTGTGCGTGAAAAAGGCGCTAAATCAATAACAATTATTGCTACACATGGTTTGTTTAATAATAATGCACGACAACATTTTCAACAAGCAATTAAAGATCGAATTATTAATAAAATTTGTATAGCTGATACAATTGAAAATGAACCTTTTGATGGTCTTGAAATTGTAAGTATCGCTCCAGCAATTGCCAAATGTATTGAAATTTATTCAGAGGGTACAGGTTCGATGTCGTTTGTACATGATGAAAATTCAAAAGTTTTATTTGCTAAAAATATATTAAATAAAAATGAATAA", "aa": "MKHYFSAYKDSSNITIVSPDYGGVKRAREISIATGATLAIVDKRRSGKNQVEINNVLGDVKDRDCVIVDDMIDTGGTILGAAKIVREKGAKSITIIATHGLFNNNARQHFQQAIKDRIINKICIADTIENEPFDGLEIVSIAPAIAKCIEIYSEGTGSMSFVHDENSKVLFAKNILNKNE", "aa_hexdigest": "aa91e9db9e1e97863cb6ac7b59d9a82b", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "prs" ], "truncated": "3-prime", "pseudogene": { "start": 689236, "stop": 690078, "observations": { "mutation": [ 689787, 689853 ], "stop": [ 689787, 689853 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQV0", "query_cov": 1.0, "subject_cov": 0.5389221556886228, "identity": 1.0, "score": 353.0, "evalue": 9.27e-121, "gene_start": 1, "gene_end": 180, "reference_start": 155, "reference_end": 334, "reference_sequence": "MSKNDDILLFSLSNSHQLANKIANLLKIELSPIRIDKFADGELIVAPQVPVRGRRVIIIQSTSKPVNDSLMELLIAIDSIKRASAKAISVVIPYYGYARQDRKAKPREPITARLVAKMIESAGATSVLTWDIHSLQTQGFFDIPFDSLEAVWVLMKHYFSAYKDSSNITIVSPDYGGVKRAREISIATGATLAIVDKRRSGKNQVEINNVLGDVKDRDCVIVDDMIDTGGTILGAAKIVREKGAKSITIIATHGLFNNNARQHFQQAIKDRIINKICIADTIENEPFDGLEIVSIAPAIAKCIEIYSEGTGSMSFVHDENSKVLFAKNILNKNE" }, "paralog": false, "identity": 0.9928571428571429, "score": 534.0, "evalue": 1e-194, "description": "Internal stop codon at 689787, 689853. Nonsense mutation around 689787, 689853.", "psc": { "uniref90_id": "UniRef90_Q9PQV0", "gene": "prs", "product": "Ribose-phosphate pyrophosphokinase", "ec_ids": [ "2.7.6.1" ], "uniref50_id": "UniRef50_Q9PQV0", "cog_id": "COG0462", "cog_category": "F", "kegg_orthology_id": "K00948", "go_ids": [ "GO:0005737", "GO:0002189", "GO:0005524", "GO:0016301", "GO:0000287", "GO:0004749", "GO:0006015", "GO:0006164", "GO:0009156" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQV0", "EC:2.7.6.1", "UniRef:UniRef50_Q9PQV0", "COG:COG0462", "COG:F", "KEGG:K00948", "GO:0005737", "GO:0002189", "GO:0005524", "GO:0016301", "GO:0000287", "GO:0004749", "GO:0006015", "GO:0006164", "GO:0009156" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQV0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQV0" ], "product": "Ribose-phosphate pyrophosphokinase" } }, "id": "NKIECMJPJD_1200", "locus": "GCA000828735_01197" }, { "type": "cds", "sequence": "contig_1", "start": 689851, "stop": 690240, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13793.12" ], "nt": "ATGTCAAAAAATGATGATATATTATTATTTAGTCTTTCAAATTCACATCAATTAGCTAATAAAATTGCTAATTTACTAAAAATTGAATTATCACCAATTAGAATTGATAAGTTTGCAGATGGTGAATTAATTGTTGCACCTCAAGTTCCAGTGCGTGGACGTCGAGTTATTATTATTCAATCAACATCTAAACCTGTTAACGATAGTTTAATGGAATTATTAATCGCTATTGACTCAATTAAACGAGCTAGTGCCAAAGCAATTAGTGTTGTAATTCCTTATTATGGATATGCACGTCAAGACCGTAAAGCTAAACCACGTGAACCAATTACAGCACGTTTAGTTGCAAAAATGATTGAGAGTGCGGGCGCAACTAGTGTATTAACTTGA", "aa": "MSKNDDILLFSLSNSHQLANKIANLLKIELSPIRIDKFADGELIVAPQVPVRGRRVIIIQSTSKPVNDSLMELLIAIDSIKRASAKAISVVIPYYGYARQDRKAKPREPITARLVAKMIESAGATSVLT", "aa_hexdigest": "9cbcf9c801550e0af72194deb8d7d997", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "psc": { "uniref90_id": "UniRef90_UPI0023F45C21", "query_cov": 0.9767441860465116, "subject_cov": 0.8811188811188811, "identity": 0.5710000000000001, "score": 135.0, "evalue": 1.86e-38, "valid": false, "product": "ribose-phosphate pyrophosphokinase", "ec_ids": [ "2.7.6.1" ], "uniref50_id": "UniRef50_A0A953RCZ7", "kegg_orthology_id": "K00948" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13793.12", "name": "Pribosyltran_N", "length": 116, "aa_cov": 0.8992248062015504, "hmm_cov": 1.0, "evalue": 2.308866835298646e-49, "score": 161.72097778320312, "start": 8, "stop": 123 } ], "seq_stats": { "molecular_weight": 14167.48779999998, "isoelectric_point": 9.923183250427243 }, "id": "NKIECMJPJD_1201", "locus": "GCA000828735_01198" }, { "type": "cds", "sequence": "contig_1", "start": 690260, "stop": 690853, "strand": "-", "frame": 2, "gene": "ytpR", "product": "YtpR family tRNA-binding protein", "db_xrefs": [ "COG:COG0073", "COG:J", "RefSeq:WP_006688899.1", "SO:0001217", "UniParc:UPI00000C1BBF", "UniRef:UniRef100_A0AAC9T070", "UniRef:UniRef50_A0A084EZ87", "UniRef:UniRef90_A0AAC9T070" ], "nt": "ATGTATATTTATTATAATAAAACAAGTCTAAACGATACGTTAATTTTTTTAAAAAACAATCAGCAATATGATCATCAAGTGTTTTTAAACGATGACTTATTGCTTTTTTATCATGGTGATGAACTAATTGGTTTAAATTTATTTAATGCAAGTGAATATTTAAAAAATTTAAATGAAGGATATTTATATCCTACGACTCAATTAATTCAAAAATTAAGTGATTTAATAAAAATAAAATTATCATTAGATAATGCTTTTAAAGGTTTTGTGGTAGGTAAAATTTTAGAAGTTAATTTAATTCCCAATACTCATTTACATATTTGCTTAGTAGATATAGGTGATCAACAAATACAAATAATATGTGGAGCACAAAATGTCCGTATTGGCTTAAAAACGGTTGTAGCGACACCTAATTTATTAATGCCAAATGGAAATGAAATTAAAAAATCCAAATTAATGAACTATGATTCATTTGGAATGTTGTGTTCACAAAAAGAATTAAATATTAATAATTTTAATTCACAAGGAATTGTCGAACTTAATGATGAATATCAGGTTGGTCAATTGTTTACAAAAGTTTATAGTAATTTATAA", "aa": "MYIYYNKTSLNDTLIFLKNNQQYDHQVFLNDDLLLFYHGDELIGLNLFNASEYLKNLNEGYLYPTTQLIQKLSDLIKIKLSLDNAFKGFVVGKILEVNLIPNTHLHICLVDIGDQQIQIICGAQNVRIGLKTVVATPNLLMPNGNEIKKSKLMNYDSFGMLCSQKELNINNFNSQGIVELNDEYQVGQLFTKVYSNL", "aa_hexdigest": "495035146bd966b30dc354ea8f261725", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI00000C1BBF", "ncbi_nrp_id": "WP_006688899.1", "uniref100_id": "UniRef100_A0AAC9T070", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1BBF", "RefSeq:WP_006688899.1", "UniRef:UniRef100_A0AAC9T070" ] }, "ips": { "uniref100_id": "UniRef100_A0AAC9T070", "uniref90_id": "UniRef90_A0AAC9T070" }, "psc": { "uniref90_id": "UniRef90_A0AAC9T070", "gene": "ytpR", "product": "YtpR family tRNA-binding protein", "uniref50_id": "UniRef50_A0A084EZ87", "cog_id": "COG0073", "cog_category": "J" }, "pscc": { "uniref50_id": "UniRef50_A0A084EZ87", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A084EZ87" ], "product": "Phenylalanine-tRNA ligase" }, "genes": [ "ytpR" ], "id": "NKIECMJPJD_1202", "locus": "GCA000828735_01199" }, { "type": "cds", "sequence": "contig_1", "start": 690998, "stop": 691375, "strand": "+", "frame": 2, "gene": "degV", "product": "DegV family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2C9DYK4", "UniRef:UniRef90_A0A2C9DYK4" ], "nt": "ATGAAAACAGCAATTTTAATCGATACAGGTGCTAACGTCTTAGATATTAAAGATGATAATGTATATTTTGTGCCTATTCAGTTAATTATTAGAGAACAACAAATAGAAAAAGAATTAAAAGATGTTATTGACGTTAGTCAAGAACAATTGAAACAAATATTAATTAATAAAACAAATATTCATACTTCATTACCTTCACCAGGTTTTGTTATGAAAAAATTAGATGAATTATTCTTAAAATATGATCGTGTTGTTATTTTAACATTATCAAGTGGTTTATCAAGTTATTTTGAATCTCTAAGTATTATTAAAGAAGATTACAAAGACAAACATTTAGTGTGCATTGATTCACGTTCTGTTTCAATTGGTATTTTATGA", "aa": "MKTAILIDTGANVLDIKDDNVYFVPIQLIIREQQIEKELKDVIDVSQEQLKQILINKTNIHTSLPSPGFVMKKLDELFLKYDRVVILTLSSGLSSYFESLSIIKEDYKDKHLVCIDSRSVSIGIL", "aa_hexdigest": "d6d045fc06237f0b5107b0f979377ad5", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "degV" ], "truncated": "3-prime", "pseudogene": { "start": 690998, "stop": 691678, "observations": { "mutation": [ 691373, 691571 ], "stop": [ 691373, 691571 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A2C9DYK4", "query_cov": 1.0, "subject_cov": 0.44014084507042256, "identity": 0.9279999999999999, "score": 226.0, "evalue": 2.58e-72, "gene_start": 1, "gene_end": 125, "reference_start": 1, "reference_end": 125, "reference_sequence": "MKTAILIDTGANVLDIKDDNVYFVPIQLIVKEHQVEQQLKDVIDVSQEQLKQILINKTSIHTSLPSPGFVMNKLDELFTKYDRVVILTLSSGLSSYFESLSIIKEDYKDKHLVCIDSRSVSIGILWLVDEINSLLKNNPSDEELIALVKERSQKIIGGVIVNNLDQLIAGGRVKKTKGIIAKALRLKLIVRWNGQLDFMDKTPNLSIAIDKLLDIINDQNQWRTNGIKNIVILTDLENESQINSLKTSLQEKIQQDVEIKVSYLPGCIYAHVGLNNFAILIEAN" }, "paralog": false, "identity": 0.9203539823008849, "score": 402.0, "evalue": 4.33e-144, "description": "Internal stop codon at 691373, 691571. Nonsense mutation around 691373, 691571.", "psc": { "uniref90_id": "UniRef90_A0A2C9DYK4", "product": "DegV family protein", "uniref50_id": "UniRef50_A0A2C9DYK4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A2C9DYK4", "UniRef:UniRef50_A0A2C9DYK4" ] }, "pscc": { "uniref50_id": "UniRef50_A0A2C9DYK4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2C9DYK4" ], "product": "DegV family protein" } }, "id": "NKIECMJPJD_1203", "locus": "GCA000828735_01200" }, { "type": "cds", "sequence": "contig_1", "start": 691592, "stop": 691852, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATAAAACACCGAATTTATCAACAGCTATAGATAAATTATTAGATATAATTGATAATCAAAATCATTGGCGCACAAATGGTATTAAGAACATTGCAATTCTTACTGATTTAGAAAATGAATCACAAATCAATTCATTAAAAACTTCATTACAAGAAAAAATTCGACAAACTATTGAAATTAAAATTTCATATTTACCGGGTTGTATTTATGCGCATGTGGGTTTAAATAATTTTGCTATCTTAATTGAAGCTAAATAG", "aa": "MDKTPNLSTAIDKLLDIIDNQNHWRTNGIKNIAILTDLENESQINSLKTSLQEKIRQTIEIKISYLPGCIYAHVGLNNFAILIEAK", "aa_hexdigest": "ca29fd1cfaa15a09bc64f31fc6cf3529", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9734.08469999999, "isoelectric_point": 6.045304298400879 }, "id": "NKIECMJPJD_1204", "locus": "GCA000828735_01201" }, { "type": "cds", "sequence": "contig_1", "start": 691926, "stop": 692447, "strand": "-", "frame": 1, "gene": null, "product": "MBA N-terminal paralog", "db_xrefs": [ "RefSeq:WP_006688938.1", "SO:0001217", "UniParc:UPI00000C1BBD", "UniRef:UniRef100_Q9PQV4", "UniRef:UniRef50_Q9PQV4", "UniRef:UniRef90_Q9PQV4" ], "nt": "ATGAAATTATTAAAAAACAAAAAAATTTGGGTATTAACAATTAGTAGTGTTTTAATTGGTGTAAGTGTTATTACATCAATTGCTTCATGCTCTAGTTCAAACATAGAATCTAAATTAAGTGATAAATTAGTTGAAGATGAAGATAATAATTATTACGTAGCATATCAAATTTCAAATTATGAAAAATTAAAAGATTTTGAAAAAAAACAATTAGAAAAAGTCACTTTTTCAACATCTATTACCCCAACTGATAAAACAAAAAACTCACAAGTTTTTAATGCAAAAGTAAATGAAGGCATTTTATTCAATCAGCAAGCGAAAACATTTTATATCAAATTAGTTCGTAAGCCTGAAGTTGGTGAAAGTATTATTATTTTACCAAATAATTCAATTTTAAAAACACACGGCTTTGTAGTTACAAATGAAAATTTAAATTTTATTAAAATTAATAAAAAAGGCGAATTACAGAGATCTAATACGGCAATAACTAAAAAAAATAATTTAACTAACGAATATAAATAG", "aa": "MKLLKNKKIWVLTISSVLIGVSVITSIASCSSSNIESKLSDKLVEDEDNNYYVAYQISNYEKLKDFEKKQLEKVTFSTSITPTDKTKNSQVFNAKVNEGILFNQQAKTFYIKLVRKPEVGESIIILPNNSILKTHGFVVTNENLNFIKINKKGELQRSNTAITKKNNLTNEYK", "aa_hexdigest": "295636eeac31ee250a8e841de7f7da43", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI00000C1BBD", "ncbi_nrp_id": "WP_006688938.1", "uniref100_id": "UniRef100_Q9PQV4", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1BBD", "RefSeq:WP_006688938.1", "UniRef:UniRef100_Q9PQV4" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQV4", "uniref90_id": "UniRef90_Q9PQV4" }, "psc": { "uniref90_id": "UniRef90_Q9PQV4", "product": "MBA N-terminal paralog", "uniref50_id": "UniRef50_Q9PQV4" }, "pscc": { "uniref50_id": "UniRef50_Q9PQV4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQV4" ], "product": "MBA N-terminal paralog" }, "genes": [], "id": "NKIECMJPJD_1205", "locus": "GCA000828735_01202" }, { "type": "cds", "sequence": "contig_1", "start": 692570, "stop": 694024, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF05000.22", "PFAM:PF04998.22" ], "nt": "ATGGCAAAATCAGGAGCTCGTGGAAACACGTCAAACTTTACACAGTTAGCAGGAATGCGTGGTTTAATGTCAAAATCATATAACTATGACCAAAAAAATAATAATGGTGTTATTAAAGATACAATTGAAATTCCAATTAAGCATTCATTTATTGAAGGATTATCAGTTAGTGAATACTTTAATTCATCATTTGGAGCACGTAAAGGGATGACTGATACGGCGATGAAAACCGCAAAATCAGGTTATATGACACGTAAGTTAGTTGATTCAACACAGGCTGTTGTAATTAAAGATCATGATTGTGGCACAAAAGAAGGTATTATTGTTCGTGAAATTCGAAATACAAAAGATAATACATCAATTGAATCACTAAAAGATCGTATTGTTGGAAGATATAGTATTAATACAATTTATGATACAAAAAATAAATTAATTATCGAAAGTGATAAATTAATTACAAGCGAAATAGCTAATATCATTCAAAATTCAGGTATTCGTGAAGTTGAGGTTCGTTCTCCATTACATTGTGCATCACTTTATGGTGTTTGTCAAAAATGTTTTGGTTTAGATCTATCAACAAATAAATTAATTGAAACCGGTACAGCGATTGGAGTAATTGCAGCGCAATCAATTGGTGAACCTGGAACACAGTTAACAATGCGTACTTTCCATACTGGAGGGGTTGCTGGTGATACGAATATTACTCAAGGGTTTGAAAGAATTAAACAATTATTTGATTGCATTCAACCACAAGAAAATGAGAAAGCAGTTATTTCACAAGTAAAAGGAACTGTTGAACGAATCGAAAAAGATTCAAACACCAATGGTTATAATGTTGTAATTAAATATAATAAAGATAATTATGTAAATTATCCAACACGTTCGAATGCTGTATTACGTGTTAAAACAGGAGATGAAATTATTGCTGGTCAAAAAATTACTGAAGGATCAATTGATGTTAATGATTTACTAAAATATGCAGGTATTGAAAATGTGCGTCATTACATTATTAAAGAAGTTCAAAAAGTTTATCGTATGCAAGGAATTGAGATTTCAGATAAGTATATTGAAGTAATCATTTCACAACTTACAAATAAAATTACAATTACAAATCCAGGAGATTCAGGATTATTTGTTGGAGAAACAATTAGTATTAATGAATTTACTGAAGTGGCTCAAAATATGCTTGTTAATAAGAAAAAACCACCAAGTGCAATTAATCAAGTATTTGGTTTAGACCATGCGCCAAGTAAATCAGGTTCATTCTTATCTGCTGCTTCGTTCCAAGATACTAAGAAAATATTAACTGACGCTGCTGCGCGTTCACAAAAAGATATGTTAATCGGCTTGAAAGAAAACGTTATTCTAGGAAATTTAATTCCTGCTGGAACAGGATTAAAGGATGTTGAAGAAGTGATTGCTTATGGTGAAGAAATGTATAAAAAGCAATACTAA", "aa": "MAKSGARGNTSNFTQLAGMRGLMSKSYNYDQKNNNGVIKDTIEIPIKHSFIEGLSVSEYFNSSFGARKGMTDTAMKTAKSGYMTRKLVDSTQAVVIKDHDCGTKEGIIVREIRNTKDNTSIESLKDRIVGRYSINTIYDTKNKLIIESDKLITSEIANIIQNSGIREVEVRSPLHCASLYGVCQKCFGLDLSTNKLIETGTAIGVIAAQSIGEPGTQLTMRTFHTGGVAGDTNITQGFERIKQLFDCIQPQENEKAVISQVKGTVERIEKDSNTNGYNVVIKYNKDNYVNYPTRSNAVLRVKTGDEIIAGQKITEGSIDVNDLLKYAGIENVRHYIIKEVQKVYRMQGIEISDKYIEVIISQLTNKITITNPGDSGLFVGETISINEFTEVAQNMLVNKKKPPSAINQVFGLDHAPSKSGSFLSAASFQDTKKILTDAAARSQKDMLIGLKENVILGNLIPAGTGLKDVEEVIAYGEEMYKKQY", "aa_hexdigest": "5dc70851e7260d133a88f1a70d91316b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF05000.22", "name": "RNA_pol_Rpb1_4", "length": 55, "aa_cov": 0.10537190082644628, "hmm_cov": 1.0, "evalue": 7.574602659725929e-12, "score": 40.71934509277344, "start": 1, "stop": 51 }, { "id": "PF04998.22", "name": "RNA_pol_Rpb1_5", "length": 340, "aa_cov": 0.6714876033057852, "hmm_cov": 0.7617647058823529, "evalue": 8.252491614074318e-62, "score": 204.57986450195312, "start": 53, "stop": 377 } ], "seq_stats": { "molecular_weight": 53352.126299999974, "isoelectric_point": 8.501010704040528 }, "id": "NKIECMJPJD_1206", "locus": "GCA000828735_01203" }, { "type": "cds", "sequence": "contig_1", "start": 694106, "stop": 694771, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF04983.23" ], "nt": "ATGATTTTAGGTATTTATTATTTAACAAAAGAAAAATTTCCACAAGTAATTGAAGAAATGATGGCTAAAGATCCAACGCAAGCGAGAGTTGAATTTATTAATCATTTTCACATTTTTTCAACACAAGACGAAGCTATTCGTGCTTATAAATTAAAAACAATTCGTATTAATGACGTAATTGGAATTACAACAAAAGCTTTTAACAATAAAACATTTAGTAAAGAAGGTATTTTGGTTACAACGGTTGGAAAAATTATCTTCAACCAAGCTTTTCCTGTTAATTTTCCATATATTAATGATGTTAAAAATCTATATGGCGAAAATCAATTTGAAATTATTGGAATGCATGAAAGTATTTTAGATTATTTAAAAGCCTATAATTTAAAAGAACCATTAACTAAAAAAACTTTATCAACAGTAATTGATTATTTATATAAAGTTTCTGAAATCGAAGTCGTACCACAAACAATGGATAAAATTAAAGCTTTAGGATTTAAATATTCAATGATTTCAGCGACAAGCATTTCTGCCTTTGATATTCCATCTTATGATCAAAAATATGAATATTTTAAAGAAACTGATGAATTGGTTTCAAAACTTCGTGAATTCTATTTAGATGGTAAATTAACTGATGATGAGCGATATACTAAAGTTGTTCAAGCTTGA", "aa": "MILGIYYLTKEKFPQVIEEMMAKDPTQARVEFINHFHIFSTQDEAIRAYKLKTIRINDVIGITTKAFNNKTFSKEGILVTTVGKIIFNQAFPVNFPYINDVKNLYGENQFEIIGMHESILDYLKAYNLKEPLTKKTLSTVIDYLYKVSEIEVVPQTMDKIKALGFKYSMISATSISAFDIPSYDQKYEYFKETDELVSKLREFYLDGKLTDDERYTKVVQA", "aa_hexdigest": "806a8c254db0734cd60f4e1898aab513", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF04983.23", "name": "RNA_pol_Rpb1_3", "length": 183, "aa_cov": 0.8144796380090498, "hmm_cov": 0.7923497267759563, "evalue": 2.932707983415123e-21, "score": 71.58325958251953, "start": 1, "stop": 180 } ], "seq_stats": { "molecular_weight": 25706.336300000017, "isoelectric_point": 5.995342826843261 }, "id": "NKIECMJPJD_1207", "locus": "GCA000828735_01204" }, { "type": "cds", "sequence": "contig_1", "start": 694823, "stop": 694966, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00623.25" ], "nt": "ATGGTGGACGGTAAAGCTATTCGTTTACATCCTCTAGTTACAACCGCTTTTAATGCGGATTTTGACGGAGACCAAATGGCTGTTCATATCCCCTTATCCAAAGAAGCAGTGGCTGAAGCACGATCAATTTTATTAGCCTCATGA", "aa": "MVDGKAIRLHPLVTTAFNADFDGDQMAVHIPLSKEAVAEARSILLAS", "aa_hexdigest": "f0eb94a243cc342762d6e08de14f3eda", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00623.25", "name": "RNA_pol_Rpb1_2", "length": 45, "aa_cov": 0.9574468085106383, "hmm_cov": 1.0, "evalue": 9.768053808232962e-19, "score": 63.76813888549805, "start": 1, "stop": 45 } ], "seq_stats": { "molecular_weight": 5020.739599999999, "isoelectric_point": 5.3670446395874025 }, "id": "NKIECMJPJD_1208", "locus": "GCA000828735_01205" }, { "type": "cds", "sequence": "contig_1", "start": 695060, "stop": 695605, "strand": "-", "frame": 2, "gene": null, "product": "DNA-directed RNA polymerase subunit", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A920VHC6" ], "nt": "ATGGTAATGACAAGAATCCCTGTCACACCATCTGAAACAAGACCAATTATTTCTTTAGATGGCGGTCGTTTTACGACCTCTGACACAAATAATTTTTATCGTAAAATCATTATTCGTAATGAACGTTTAAAACAAATGCAAGCAACAGATGCTCCTGAAATTTTATTAGATAATGAAAAACGTTTATTACAAGAAGCAGTTGATTCATTATTTGATAATAATTCACGTAAAAAACCAGTTGTAGGTAAAGATAAACGTCCTTTAAAATCATTGTCAAATCATCTTAAAGGAAAACAAGGATTATTTAGACAAAATTTATTAGGAAAACGTGTTGACTATTCAGGTCGTTCTGTAATTGTTGTTGGTCCTGAATTAAAAATGTATGAAGTAGGAATTCCTGCTTTAATGATTTTAAAATTATTTAGACCATATATTATTTCTGAATTAATACGTAAACGTGATGAATTAGGTAATGAAATTCAACCAATTTGTGCCAATATTAAATTAGCAGAACAAAAAATTCTTGCGCAAGATAATGAGATTTGA", "aa": "MVMTRIPVTPSETRPIISLDGGRFTTSDTNNFYRKIIIRNERLKQMQATDAPEILLDNEKRLLQEAVDSLFDNNSRKKPVVGKDKRPLKSLSNHLKGKQGLFRQNLLGKRVDYSGRSVIVVGPELKMYEVGIPALMILKLFRPYIISELIRKRDELGNEIQPICANIKLAEQKILAQDNEI", "aa_hexdigest": "6b086cf32e129c28d10eaf823f1216c2", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A920VHC6", "query_cov": 0.8397790055248618, "subject_cov": 0.9440993788819876, "identity": 0.586, "score": 179.0, "evalue": 7.9e-55, "valid": false, "product": "DNA-directed RNA polymerase subunit", "uniref50_id": "UniRef50_A0A920VHC6" }, "pscc": { "uniref50_id": "UniRef50_A0A920VHC6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A920VHC6" ], "product": "DNA-directed RNA polymerase subunit" }, "genes": [], "id": "NKIECMJPJD_1209", "locus": "GCA000828735_01206" }, { "type": "cds", "sequence": "contig_1", "start": 695636, "stop": 695863, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAAAAGATTCTAATATTCCTTTTTCAGTGAAAGATGTAATGGCTTTTATTGAAAAACATACAGGCGTTCGTTTTGGTATTGGTGCTGAAGCTATTCGTGAATTATTAGAAAAACTAAATCTTGAAGAAGAACATGAAAAGATTAAACAAGCAATTCAAAATTCACCTAATGCTTATGATCAAAAAACAAAACGCTTATTGCGTCGTTTAGAATGTGTAAGATGA", "aa": "MLKDSNIPFSVKDVMAFIEKHTGVRFGIGAEAIRELLEKLNLEEEHEKIKQAIQNSPNAYDQKTKRLLRRLECVR", "aa_hexdigest": "902af672a3149d54c75af49d2c7d4e62", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8738.046099999992, "isoelectric_point": 8.851718711853028 }, "id": "NKIECMJPJD_1210", "locus": "GCA000828735_01207" }, { "type": "cds", "sequence": "contig_1", "start": 696176, "stop": 696355, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001EDE8919", "PFAM:PF04997.17" ], "nt": "TTGTTTGATGAAAGTATTTTTGGGCCTTCAAAAGATTATGAATGTTATTGTGGAAAATATCGTAAAGTAAAACATAAAGGTAAAATTTGTGAACGTTGTCATGTTGAAATTACTGAATCAATTGTACGTCGTGAACGAATGGGACATATTGAGTTAGCTGCTCCTGTTGCCCATATTTGA", "aa": "MFDESIFGPSKDYECYCGKYRKVKHKGKICERCHVEITESIVRRERMGHIELAAPVAHI", "aa_hexdigest": "903f56e9f727fcfd1b7191ab57fbb5af", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001EDE8919", "query_cov": 0.9830508474576272, "subject_cov": 0.8055555555555556, "identity": 0.7240000000000001, "score": 95.1, "evalue": 1.39e-24, "valid": false, "uniref50_id": "UniRef50_UPI001EDE8919" }, "pscc": { "uniref50_id": "UniRef50_UPI001EDE8919", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001EDE8919" ] }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF04997.17", "name": "RNA_pol_Rpb1_1", "length": 60, "aa_cov": 1.0, "hmm_cov": 0.6833333333333333, "evalue": 3.822361220966815e-12, "score": 41.686378479003906, "start": 1, "stop": 59 } ], "seq_stats": { "molecular_weight": 6895.970699999998, "isoelectric_point": 8.413913917541507 }, "id": "NKIECMJPJD_1211", "locus": "GCA000828735_01208" }, { "type": "cds", "sequence": "contig_1", "start": 696509, "stop": 696751, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCAGAATCATTTAAATTATTAACAAAACAAATGCAAGGTTTAGGGTTATGTATTACCGTTGAAACAAAAGATGATCGTATGGTAGATATTAATGAATATACACTAAATCAAAATCGTTTAAATAATGACGATGATGAGGTTATTTTAGATGAAAATCTAAAAGAGATCAATGATTCTAATGAAGAAATATTTAATACAAACTTTAATAATAATGACTATGATGATGAAGAGAACTTCTAA", "aa": "MPESFKLLTKQMQGLGLCITVETKDDRMVDINEYTLNQNRLNNDDDEVILDENLKEINDSNEEIFNTNFNNNDYDDEENF", "aa_hexdigest": "49a511866c6a46715b33956c357144ac", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9453.107499999995, "isoelectric_point": 4.0500284194946286 }, "id": "NKIECMJPJD_1212", "locus": "GCA000828735_01209" }, { "type": "cds", "sequence": "contig_1", "start": 696878, "stop": 697948, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00562.33" ], "nt": "ATGAAAACAAAAAACGGTGATGAAGAAATCACTCGTGACATGCCTAATGTCTCTGATACTGCAAAACGTTTTTTAGATAATCAAGGTATCGTTTTAGTTGGCGCTGAAGTTCATGAAGGAGATGTTCTAGTTGGAAAAACAACTCCACGAGGTAATGTTGAAACTGCTCCAGAAGATCGTTTATTGCAAACAATTTTTGGCGATAAATCAAAAACAGTTAAAGATTCATCACTAAAAGTAAAACACGGTCAAGAAGGAATTGTTGCTGCTGTTAAGCGAATTAAATCAAGTGATGAAAATGGTAGTGAATTACCAGATGACGTAATTGAAATTATCAAAGTTTATATTGTTCAAAAACGTAAAATTCAAGTTGGTGACAAAATGGCAGGGCGTCATGGAAATAAAGGAATTGTATCAAAAGTTGTTCCTATTCAAGATATGCCTTTTTTAAAAGACGGGACTCCATTAGATATTATGTTAAATCCATTAGGTGTACCTAGTCGTATGAATATTGGTCAAATTTTAGAATTGCACTTAGGTTATGCTGCGGCTGAAATAGGTAAAAAACAATTAATTCAAATTGCAATTGATCAATTAGGTTATGAAAAATATATTTCATTGTTTGGAATCAATGAAATTATTGCTAAAAAACTTTATGAAAATATTAGTAATTTAATTAAACACAAACAAGCAAAACAGGCAAAAGATATAGATTTAATTGATGTGACAATTATTCTAAAAGAATTGGGATTATCATATGATGATATTGGAATTAAAATTTCAACTCCTGTATTTGATGGTGCAAATCATGATGATATTGTTTCTATTATGAATGAAGCAAACATTGATATTGAAAATAATAAGGGTAAACAAGTTCTATATGATGGAAGAACTGGTGAACCATTCGATGGTTTAATTTCAGTTGGATTAACATACATGTTAAAACTAGATCATATGGTTGATGATAAAATTCACAGTCGTTCGGTTGGTCCATATAGTAAAATTACACAACAACCATTGGGTGGTAAATCACAAAATGGAGGCCAACGTTTTGGTGAAATGGAAGTTTGA", "aa": "MKTKNGDEEITRDMPNVSDTAKRFLDNQGIVLVGAEVHEGDVLVGKTTPRGNVETAPEDRLLQTIFGDKSKTVKDSSLKVKHGQEGIVAAVKRIKSSDENGSELPDDVIEIIKVYIVQKRKIQVGDKMAGRHGNKGIVSKVVPIQDMPFLKDGTPLDIMLNPLGVPSRMNIGQILELHLGYAAAEIGKKQLIQIAIDQLGYEKYISLFGINEIIAKKLYENISNLIKHKQAKQAKDIDLIDVTIILKELGLSYDDIGIKISTPVFDGANHDDIVSIMNEANIDIENNKGKQVLYDGRTGEPFDGLISVGLTYMLKLDHMVDDKIHSRSVGPYSKITQQPLGGKSQNGGQRFGEMEV", "aa_hexdigest": "69fbb94408893c010217735acf4a718a", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00562.33", "name": "RNA_pol_Rpb2_6", "length": 347, "aa_cov": 0.9662921348314607, "hmm_cov": 0.7550432276657061, "evalue": 1.3330520749075404e-97, "score": 322.7413024902344, "start": 2, "stop": 345 } ], "seq_stats": { "molecular_weight": 39178.53360000003, "isoelectric_point": 5.96033000946045 }, "id": "NKIECMJPJD_1213", "locus": "GCA000828735_01210" }, { "type": "cds", "sequence": "contig_1", "start": 698048, "stop": 699280, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF04561.19", "PFAM:PF04565.21", "PFAM:PF10385.14", "PFAM:PF00562.33" ], "nt": "GTGGCTGACTTAGTTGCTACAACAAGTTATATTTATAACTTGAATTATGAAATTGGTGAATTTGATGATATAGATCATTTAGGTAATAAAAGACTTAAATTGATTCATGAATTATTACGAGCAAGAATTGCTACAAGTATGGCTCGTATTGAGAAGTTTATTAATGAAAAGTTGGCTATTTCTGATGGTTCAAGCAATAACATTACAAATGTTAATGATAAAGGTATTGATACTGAACTTGATCGTGAAATTGAAGAAAGCGATATGAGTGATGAGGAAAAGAAAAAAGCAATTAGTGTTAAATCAATTATTAATACAAAACAATTCCAAAGTTTAGTTAAAGATTTTTTTAATTCACATCAATTAATTCAATTTATTGACCAACAAAATCCATTAGCAGAATTAACTAATAAACGTCGTATTTCAGCGATGGGTCCAGGAGGAATTAGTCGTGAAGACCCTAATTTAGATATTCGTGATGTTCATCACTCTCACTATTCGCGTATTTGCCCGATTGAAACACCAGAAGGAATGAATATTGGGTTGATTATGTCTTTAGCATCACTTGCAAAAGTAGATGAAAATGGTTTTATTGTAGCACCTTATTATGTTGTTGAGGATGGGGTTGTAAAAGAAGATTATAAATATTTAACAGCCCATGAAGATGATAACTATATTATTGCTGAATCATCAGTCCAATTAGATGAAAATAAACGAATTTTAGATGAACAAGTTGTTGCTCGATATCGTGGCTCAACAGGTTTATTTAGTCCTAATGAAGTTGATTTTATTGATATTGTTCCAAAACAAGTAGTATCAATTGCTGCATCAGCAATTCCATTTATTGAAAATGACGATGGGGCACGTGCTTTGATGGGGTCAAACATGCAACGTCAAGCAACACCTTTAATTAAACCATATGCCCCTATTGTAGGGACAGGTACAGAATTTAAAATTGCACACGATTCAGGAATGGCTGTTGTTGCTAAAAATGACGGAGTGGTTGAATTTGTTGATAGTCAAAAAATTATCATTAGAAATGATAATGATAAATTAGATGATTATAAATTAATTAAATATCGTAAATCAAATCAAGACACATGTAATAACCAAATTCCAATTGTAAAAGTAGGTCAAAGAGTTCACAAATCTGAAACAATTGGTGATGGACCTGCAATGCAAAATGGTGAATTAGCTTTAGGTCGTAATATTCTAGTTGGTTATACAACTTGA", "aa": "MADLVATTSYIYNLNYEIGEFDDIDHLGNKRLKLIHELLRARIATSMARIEKFINEKLAISDGSSNNITNVNDKGIDTELDREIEESDMSDEEKKKAISVKSIINTKQFQSLVKDFFNSHQLIQFIDQQNPLAELTNKRRISAMGPGGISREDPNLDIRDVHHSHYSRICPIETPEGMNIGLIMSLASLAKVDENGFIVAPYYVVEDGVVKEDYKYLTAHEDDNYIIAESSVQLDENKRILDEQVVARYRGSTGLFSPNEVDFIDIVPKQVVSIAASAIPFIENDDGARALMGSNMQRQATPLIKPYAPIVGTGTEFKIAHDSGMAVVAKNDGVVEFVDSQKIIIRNDNDKLDDYKLIKYRKSNQDTCNNQIPIVKVGQRVHKSETIGDGPAMQNGELALGRNILVGYTT", "aa_hexdigest": "6832ce2f90fbc9186dc3fccc93a3c7f7", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF04561.19", "name": "RNA_pol_Rpb2_2", "length": 31, "aa_cov": 0.07560975609756097, "hmm_cov": 1.0, "evalue": 5.237650527770396e-07, "score": 25.056922912597656, "start": 3, "stop": 33 }, { "id": "PF04565.21", "name": "RNA_pol_Rpb2_3", "length": 69, "aa_cov": 0.16829268292682928, "hmm_cov": 0.9855072463768116, "evalue": 8.726591504782214e-27, "score": 88.71739959716797, "start": 124, "stop": 192 }, { "id": "PF10385.14", "name": "RNA_pol_Rpb2_45", "length": 65, "aa_cov": 0.15853658536585366, "hmm_cov": 1.0, "evalue": 5.121129508493801e-22, "score": 73.3185806274414, "start": 202, "stop": 266 }, { "id": "PF00562.33", "name": "RNA_pol_Rpb2_6", "length": 87, "aa_cov": 0.2, "hmm_cov": 0.8850574712643678, "evalue": 1.9676569793432217e-12, "score": 42.461307525634766, "start": 328, "stop": 409 } ], "seq_stats": { "molecular_weight": 45809.18350000003, "isoelectric_point": 5.043517112731934 }, "id": "NKIECMJPJD_1214", "locus": "GCA000828735_01211" }, { "type": "cds", "sequence": "contig_1", "start": 699437, "stop": 700813, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAAATAATAAAAATTATACTGAGAAATTTATTACTGATAAAGTAATGCGAAGAGATTATTCAAAAATTAAATCTAATTTTGAAGGACCAAATCTACTTGAAATTCAAGTTGAATCTTTTAAACGCTTTATGGAGAAAGATTTAAAAGAAGTTATTTCAAGTATTTTTCCATTAAAATCTCCTCAGGGTAAGTATACGTTAGCATTTAAAGGTTTAAAAATTAAGCAGCCAACAAAAGATGAAAGTGCTTGTCGTGACGAAGGTAAAACATTTGAAACACCAATTTATATTGATTTGGAATTAACAGATAACTACACAGGTGAAGTTAAACGTGCACAACGAAATACTAAAACAGGAGAAGATGGTATTTATTTAGGAGCAATTCCCAAAATGACAGAAAAAGGAACTTTTGTTATTAATGGAATTGAAAAATTTGTTATCTCACAAATTGTTAGATCACCAGGAATTTATGTACTTGGTAAATCAAGCATCAAATTGAACGGTTCACGTAAGCGTTTATTTGAAGGCAAAATTTGTGAAATTTATCCTTCTAAGGGTACTTTGATGCTTGGTTATATTCCTAAGGATCGTCATAATATTCAAATTGTTGCACGTGATTCATCTGGTGATAATGCGCAAACATTTTCAGTAACTACTTTACTAAAAGCTTTTGGTTTAACAAGTGCTGAAATCTTAAAAATTTTTAATAATGAAAAAGAGATTCGTGAATCATTAGAAATTGAAAAATATGCGCCAGAATATATTTTTGAAAATTCACAAGAAAATGAAATTATTTTTAAAATTTATAGTGATGCTCATGATATTCATGAGAAAGCCGATCGTCGAGAATCAAATAATAAGTTAAAAGAAGAATATATAGAACAAGGTTCACCACTTTTATCAAAGTTAAAACAACTAATTTTTAATTATGTTGAAAAAAACGATGAAATTGATGCATTATTACATGAAAATAAAGATGTTGAAGATGCTAGTTTCATTAAGAATAATAAAAAATTATATGATGAAAGAGAAGAAATCATTAATTGCATCATTAGTGAAAAAGCAGCTAAGGATATCGTTGAATTATTAGGTATTAATATTAAAAATATAGAAACACTAAGACATTTAGGCAAAGCTTCTTATCAAATAGCTTTACAACAACATTTTTTTAATAAGCGTTTATATGATATTAGTAGTGCTGGACGCTATAAATTTGAAAAAAAATTATTATTAAGTGAACGTTTATATCAAAAAGTAATTGCGAATGATATTATTGATAAAAAAAATAATATTCTAATTCCTAAAGATACTTTAATTACTAAAGAACATATCGAACTAATAAAAAAAGAATCACGTGATAAAAACATTAAATGA", "aa": "MQNNKNYTEKFITDKVMRRDYSKIKSNFEGPNLLEIQVESFKRFMEKDLKEVISSIFPLKSPQGKYTLAFKGLKIKQPTKDESACRDEGKTFETPIYIDLELTDNYTGEVKRAQRNTKTGEDGIYLGAIPKMTEKGTFVINGIEKFVISQIVRSPGIYVLGKSSIKLNGSRKRLFEGKICEIYPSKGTLMLGYIPKDRHNIQIVARDSSGDNAQTFSVTTLLKAFGLTSAEILKIFNNEKEIRESLEIEKYAPEYIFENSQENEIIFKIYSDAHDIHEKADRRESNNKLKEEYIEQGSPLLSKLKQLIFNYVEKNDEIDALLHENKDVEDASFIKNNKKLYDEREEIINCIISEKAAKDIVELLGINIKNIETLRHLGKASYQIALQQHFFNKRLYDISSAGRYKFEKKLLLSERLYQKVIANDIIDKKNNILIPKDTLITKEHIELIKKESRDKNIK", "aa_hexdigest": "b26cf638b776eee893d209f2d2e5971b", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 53023.19719999994, "isoelectric_point": 8.823997306823731 }, "id": "NKIECMJPJD_1215", "locus": "GCA000828735_01212" }, { "type": "cds", "sequence": "contig_1", "start": 700936, "stop": 702360, "strand": "-", "frame": 3, "gene": "pyk", "product": "pyruvate kinase", "db_xrefs": [ "COG:COG0469", "COG:G", "EC:2.7.1.40", "GO:0000287", "GO:0004743", "GO:0006096", "GO:0030955", "KEGG:K00873", "RefSeq:WP_006689159.1", "SO:0001217", "UniParc:UPI00000C1BBC", "UniRef:UniRef100_Q9PQV7", "UniRef:UniRef50_Q9PQV7", "UniRef:UniRef90_Q9PQV7" ], "nt": "ATGTTTTCAAAAACACGCATTATTGCTACAGTAGGTCCAGCAACAACAAAATTGTTTTCTAATTGGTTAGAATTTAACGATTTAAATAATATAAATGATGCTCAACAAGTATGCATAAACATTAAAGATTTAATTTTAAGTGGTGTTAATATTTTTCGTATGAATTTAAGTCATGGCGACCAAAAAATACACCTTTTTCGTACACAATTAATTAAAAAAATTGCGGATGAATTAAAGATCAAAGTTGAAATATTATTTGATACAAAAGGACCAGAAATTCGTGTTTGTGAAATGAGTGATAATAATTTTATTATTAAAAATAGTGAAGTTATAATACATTGTAAAGAAAAAGTTTTAGGTAGTTTTAATGAGTTTAGTGTTACTGATGCTACTGGACAATACAATATGATTAGTGATGTTAAAATTAATCACCGAATTTTAATCGACGATGGAAAATTAATTTTAATTGTTAAAAAAATTGATTTTCTAAAAAACATCATTTATACAACCGCTAAAAATAGTTATTCATTAAAAACAAATAAACGCTTAAATTTACCAGATGCCAATTATAGTTTGCCGTTTTTGTCAAAGAAAGATATTGATGATATAAATTTAGCAGTTAAATTAAAAATTCCTTATTTGGCTTTATCGTTTATTTCTAATATTAAACAAATTAATGAGGTTAAACAACTTTTAAAAAAGTCCTCATTTATACCAAAATTAATTGCAAAAATTGAAACTCAAGAAGCAATTGATAATCTAGAAGAAATTATTAAAAATACTGATGGTATCATGGTTGCGCGTGGGGATTTAGGGCTAGAAGTACCTTTTTATAAAATTCCTATTTATCAAAATAAAATTGTTGAATTATGCCATAAATACAATAAATATTGTATTATAGCGACACAAATGTTAGATTCATTAGAACGTAATATTATCCCCACACGAGCTGAAGTCACAGATGTTTATTATGCCGTTAAACAAAAGGTTAACGCAACAATGTTATCAGGCGAAACGGCCGCAGGAATTGATCCAATTAATGCTGTACAAGTTATGAAATCTATTATTTTAGAGACAGAAAATAATAATATAGAAAACGAAATAACATTTAAAAAAAATGAATTATTAATTCCTAAGGAAGTTAAATTAAAAATTTTATCATTTCATAAATTAAAAATAAATAAACATAAATTATTAGTTGTTAATTTAATTAGTAAAAATTCTAAACATTTAGTTTATGATATATTAAAATATGATTTTAATTTTAATATTTTATTTGTTATAGATAAAAATCAAGAACATGATTTATTATTAAAAAATGCTAATTTTATTATTAGTGAAAATAAAATGTTAAAACAAGAAGAATTATTAAAATCATTTAATATTGATTTTAATAATCATGAATTTTTATTTATTTCAATTTAA", "aa": "MFSKTRIIATVGPATTKLFSNWLEFNDLNNINDAQQVCINIKDLILSGVNIFRMNLSHGDQKIHLFRTQLIKKIADELKIKVEILFDTKGPEIRVCEMSDNNFIIKNSEVIIHCKEKVLGSFNEFSVTDATGQYNMISDVKINHRILIDDGKLILIVKKIDFLKNIIYTTAKNSYSLKTNKRLNLPDANYSLPFLSKKDIDDINLAVKLKIPYLALSFISNIKQINEVKQLLKKSSFIPKLIAKIETQEAIDNLEEIIKNTDGIMVARGDLGLEVPFYKIPIYQNKIVELCHKYNKYCIIATQMLDSLERNIIPTRAEVTDVYYAVKQKVNATMLSGETAAGIDPINAVQVMKSIILETENNNIENEITFKKNELLIPKEVKLKILSFHKLKINKHKLLVVNLISKNSKHLVYDILKYDFNFNILFVIDKNQEHDLLLKNANFIISENKMLKQEELLKSFNIDFNNHEFLFISI", "aa_hexdigest": "6f5a70da9a51119ed1b32bb923540790", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI00000C1BBC", "ncbi_nrp_id": "WP_006689159.1", "uniref100_id": "UniRef100_Q9PQV7", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1BBC", "RefSeq:WP_006689159.1", "UniRef:UniRef100_Q9PQV7" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQV7", "uniref90_id": "UniRef90_Q9PQV7" }, "psc": { "uniref90_id": "UniRef90_Q9PQV7", "gene": "pyk", "product": "pyruvate kinase", "ec_ids": [ "2.7.1.40" ], "uniref50_id": "UniRef50_Q9PQV7", "cog_id": "COG0469", "cog_category": "G", "kegg_orthology_id": "K00873", "go_ids": [ "GO:0000287", "GO:0004743", "GO:0006096", "GO:0030955" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQV7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQV7" ], "product": "Pyruvate kinase" }, "genes": [ "pyk" ], "id": "NKIECMJPJD_1216", "locus": "GCA000828735_01213" }, { "type": "cds", "sequence": "contig_1", "start": 702612, "stop": 702740, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAACTAATCAAGATAATAAATATACTGTTGATGAAGTAATAGATTTAATTAAAGATAAAATAAACTCAATAAAAGAATACGATCCTTTAATCATTGTTCAAGAATTAATCTATGATCAAAAATGA", "aa": "MLTNQDNKYTVDEVIDLIKDKINSIKEYDPLIIVQELIYDQK", "aa_hexdigest": "4bbe38bcb1985267934f0350e51b8ec4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5012.681100000001, "isoelectric_point": 4.312624549865723 }, "id": "NKIECMJPJD_1217", "locus": "GCA000828735_01214" }, { "type": "cds", "sequence": "contig_1", "start": 702780, "stop": 703337, "strand": "-", "frame": 1, "gene": "pfkA", "product": "putative ATP-dependent 6-phosphofructokinase", "db_xrefs": [ "COG:COG0205", "COG:G", "EC:2.7.1.11", "GO:0003872", "GO:0005524", "GO:0005945", "GO:0006002", "GO:0016208", "GO:0030388", "GO:0042802", "GO:0046872", "GO:0048029", "GO:0061621", "GO:0070095", "SO:0001217", "UniRef:UniRef50_Q9PQV8", "UniRef:UniRef90_Q9PQV8" ], "nt": "ATGAACCAAGTAAATTTTTTAAATTTAGATAAAAATATTTTAATTATTACTTCAGGTGGTGATGCACCGGGTATGAATGCTTCATTAATTTCATTAATCCATAGATTAATGAATAATAATTTTAATGTTTTTATAGGAATTGAAGGATTATTAGGACTGTACAATAATTTAATTGAACCAATTAAAGATAAACGTGTTTTTGATGTTTATTTTAACGAGCAAGGAACAGTCATTAAGACAAGTAGATTTATTAAATTAGATATTAATGATAAAAAAACACAAATTATTAAAGATAATTTATTAAGTCATAATATTCAAAAAATTATTATCTTAGGCGGCCAAGGTAGTATGCAAGCTGGTTTAGTGTTAACAAAAATGGGCTTTGAAGTCTTTGGAATATTACACACTATTGATAATGATTTTAGTGAAACACAAATGTGTATAGGGGCCTTAAGTGCTGCTTCTTTTAATCAAAAATTATTAAAGTGCCTAAATTACACAGCAAAAGCTCATTGTGCTTTTAATTTAGTTGAGCTTATGGGTCGTGAATGTTCATGA", "aa": "MNQVNFLNLDKNILIITSGGDAPGMNASLISLIHRLMNNNFNVFIGIEGLLGLYNNLIEPIKDKRVFDVYFNEQGTVIKTSRFIKLDINDKKTQIIKDNLLSHNIQKIIILGGQGSMQAGLVLTKMGFEVFGILHTIDNDFSETQMCIGALSAASFNQKLLKCLNYTAKAHCAFNLVELMGRECS", "aa_hexdigest": "99ad1d3c7279f89ee0143851fa0256b4", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "genes": [ "pfkA" ], "truncated": "5-prime", "pseudogene": { "start": 702477, "stop": 703337, "observations": { "mutation": [ 702614, 702782 ], "stop": [ 702614, 702782 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQV8", "query_cov": 1.0, "subject_cov": 0.5763239875389408, "identity": 1.0, "score": 368.0, "evalue": 6.01e-127, "gene_start": 1, "gene_end": 185, "reference_start": 1, "reference_end": 185, "reference_sequence": "MNQVNFLNLDKNILIITSGGDAPGMNASLISLIHRLMNNNFNVFIGIEGLLGLYNNLIEPIKDKRVFDVYFNEQGTVIKTSRFIKLDINDKKTQIIKDNLLSHNIQKIIILGGQGSMQAGLVLTKMGFEVFGILHTIDNDFSETQMCIGALSAASFNQKLLKCLNYTAKAHCAFNLVELMGRECSWLVNNSVGKLKPILMLTNQDNKYTVDEVIDLIKDKINSIKEYDPLIIVQELIYDQKWYELLVKTFEQKLHKSLRITILNYLQRGAPVSDFDLQLAKDSANVLVDFIVNQNDIKNTNNMYVVINKFDNKLEVIKFNK" }, "paralog": false, "identity": 0.993006993006993, "score": 555.0, "evalue": 3.21e-203, "description": "Internal stop codon at 702614, 702782. Nonsense mutation around 702614, 702782.", "psc": { "uniref90_id": "UniRef90_Q9PQV8", "gene": "pfkA", "product": "Probable ATP-dependent 6-phosphofructokinase", "ec_ids": [ "2.7.1.11" ], "uniref50_id": "UniRef50_Q9PQV8", "cog_id": "COG0205", "cog_category": "G", "go_ids": [ "GO:0005945", "GO:0003872", "GO:0016208", "GO:0005524", "GO:0070095", "GO:0042802", "GO:0046872", "GO:0048029", "GO:0061621", "GO:0030388", "GO:0006002" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQV8", "EC:2.7.1.11", "UniRef:UniRef50_Q9PQV8", "COG:COG0205", "COG:G", "GO:0005945", "GO:0003872", "GO:0016208", "GO:0005524", "GO:0070095", "GO:0042802", "GO:0046872", "GO:0048029", "GO:0061621", "GO:0030388", "GO:0006002" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQV8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQV8" ], "product": "Probable ATP-dependent 6-phosphofructokinase" } }, "id": "NKIECMJPJD_1218", "locus": "GCA000828735_01215" }, { "type": "cds", "sequence": "contig_1", "start": 703306, "stop": 703662, "strand": "-", "frame": 3, "gene": null, "product": "Enolase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2E9FKC7" ], "nt": "GTGGTTGGTGATGATTTATATTGCACAAACTTAAAGTATCTACAAAAAGGAATTGATTTTAAAGCAACAAATGCTATTTTAATTAAACCTAATCAAATTGGTACAATTAGTGAAACGTTAGATGTTATTAAATTTGCACAAAAAAACAATATTAATACTATCATTTCGCATCGCTCTGGCGAAACTGAAGACACTTTTATTGCAGATTTAGCGATTGGTGTAGGTGCTGGGCAAATTAAAACAGGTTCATTATCACGTTCTGAACGGATAGCGAAGTATAATCGTATTTTAGAAATCGAACAAGAATTAAAGGATAAATTAATTTATGAACCAAGTAAATTTTTTAAATTTAGATAA", "aa": "MVGDDLYCTNLKYLQKGIDFKATNAILIKPNQIGTISETLDVIKFAQKNNINTIISHRSGETEDTFIADLAIGVGAGQIKTGSLSRSERIAKYNRILEIEQELKDKLIYEPSKFFKFR", "aa_hexdigest": "5c090ee2e7eb53d2794490c217aa003b", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A2E9FKC7", "query_cov": 0.8389830508474576, "subject_cov": 0.8181818181818182, "identity": 0.6970000000000001, "score": 142.0, "evalue": 1.62e-41, "valid": false, "product": "Enolase", "uniref50_id": "UniRef50_A0A2E9FKC7" }, "pscc": { "uniref50_id": "UniRef50_A0A2E9FKC7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2E9FKC7" ], "product": "Enolase" }, "genes": [], "id": "NKIECMJPJD_1219", "locus": "GCA000828735_01216" }, { "type": "cds", "sequence": "contig_1", "start": 703780, "stop": 704628, "strand": "-", "frame": 3, "gene": "eno", "product": "phosphopyruvate hydratase", "db_xrefs": [ "COG:COG0148", "COG:G", "EC:4.2.1.11", "GO:0000015", "GO:0000287", "GO:0004634", "GO:0005576", "GO:0006096", "GO:0009986", "KEGG:K01689", "SO:0001217", "UniRef:UniRef50_Q9PQV9", "UniRef:UniRef90_Q9PQV9" ], "nt": "ATGAAAATAATTAATTTATTAGCTTATCAAATTCTTGATTCGCGCGGCCAACCAACAGTAGCTGTTAAATTATTTTTAGAGAACGATCAAAGTGTAATTGCAATGGTTCCATCAGGCGCTTCCACTGGAGCAAAGGAAGCATTAGAATTAAGAGATGGTGATGTAAATTATTTTTTTAATAAGTCTGTTAAATTAGCAATTCAAAATATTAATAATATTATTCGCCCTCATCTAATTAATAAAAATGTTTTAAATTTTTTTGAATTAGATAATTTATTAATTAATCTAGATGGTACTGAAAATAAAAGTAAATTAGGTGCTAATGCTTTATTAGGTGTTTCTATAGCGATTGTTAAAGCGGGAGCAATAGCCGCTTCTAAACCTTTATATCAATATATTAAAGAAGACTTAATGCATAATTATGATGTAAACTATTATGCTCCAATTCCGTTAATGAATTTTATTAATGGTGGGGCTCACGCAGATAATGATTTAGATATTCAAGAATTTATGATTGTTCCATTAAATGCAATTTCATTTTCTCAAGCGATTCAAATTGGATCAGAAATTTTTCATCAATTAGATAAACTTTTAAAAAGCAATCATTTAAGTACAACAAAGGGTGATGAAGGCGGTTTTGCTCCAATGTTAAAAAATAATTATGTGACTTTAGAACTATTAGTTCATGCTATTAAAAAAGCACATTACCTTCCTTCAAAAACACAAGGAGTTTGTCTTGCCTTAGATGTCGCAGCTTCTGAATTGTATGAAAACGGAAAATATTTTTTTAAAAAGTCTTCATCTCATAACATTACTCTTGAACAAACATCATTCAGTAGTGATGAATGA", "aa": "MKIINLLAYQILDSRGQPTVAVKLFLENDQSVIAMVPSGASTGAKEALELRDGDVNYFFNKSVKLAIQNINNIIRPHLINKNVLNFFELDNLLINLDGTENKSKLGANALLGVSIAIVKAGAIAASKPLYQYIKEDLMHNYDVNYYAPIPLMNFINGGAHADNDLDIQEFMIVPLNAISFSQAIQIGSEIFHQLDKLLKSNHLSTTKGDEGGFAPMLKNNYVTLELLVHAIKKAHYLPSKTQGVCLALDVAASELYENGKYFFKKSSSHNITLEQTSFSSDE", "aa_hexdigest": "41e75c27ce1dbf706a866b914bc7be4a", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_UPI0026067ABB", "query_cov": 0.8900709219858156, "subject_cov": 0.8508474576271187, "identity": 0.518, "score": 239.0, "evalue": 4.98e-75, "valid": false, "gene": "eno", "product": "phosphopyruvate hydratase", "ec_ids": [ "4.2.1.11" ], "uniref50_id": "UniRef50_A0A838WLF1", "kegg_orthology_id": "K01689", "go_ids": [ "GO:0000015", "GO:0000287", "GO:0004634", "GO:0006096" ] }, "genes": [ "eno" ], "truncated": "5-prime", "pseudogene": { "start": 703477, "stop": 704628, "observations": { "mutation": [ 703707, 703770, 703782 ], "stop": [ 703707, 703770, 703782 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQV9", "query_cov": 1.0, "subject_cov": 0.6409090909090909, "identity": 1.0, "score": 549.0, "evalue": 8.59e-195, "gene_start": 1, "gene_end": 282, "reference_start": 1, "reference_end": 282, "reference_sequence": "MKIINLLAYQILDSRGQPTVAVKLFLENDQSVIAMVPSGASTGAKEALELRDGDVNYFFNKSVKLAIQNINNIIRPHLINKNVLNFFELDNLLINLDGTENKSKLGANALLGVSIAIVKAGAIAASKPLYQYIKEDLMHNYDVNYYAPIPLMNFINGGAHADNDLDIQEFMIVPLNAISFSQAIQIGSEIFHQLDKLLKSNHLSTTKGDEGGFAPMLKNNYVTLELLVHAIKKAHYLPSKTQGVCLALDVAASELYENGKYFFKKSSSHNITLEQTSFSSDEWIKYWSKLVSMFPIISIEDCFEENDWNSFALFLKNNPHIQVVGDDLYCTNLKYLQKGIDFKATNAILIKPNQIGTISETLDVIKFAQKNNINTIISHRSGETEDTFIADLAIGVGAGQIKTGSLSRSERIAKYNRILEIEQELKDKLIYEPSKFFKFR" }, "paralog": false, "identity": 0.9921671018276762, "score": 744.0, "evalue": 4.14e-274, "description": "Internal stop codon at 703707, 703770, 703782. Nonsense mutation around 703707, 703770, 703782.", "psc": { "uniref90_id": "UniRef90_Q9PQV9", "gene": "eno", "product": "phosphopyruvate hydratase", "ec_ids": [ "4.2.1.11" ], "uniref50_id": "UniRef50_Q9PQV9", "cog_id": "COG0148", "cog_category": "G", "kegg_orthology_id": "K01689", "go_ids": [ "GO:0000015", "GO:0000287", "GO:0004634", "GO:0005576", "GO:0006096", "GO:0009986" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQV9", "EC:4.2.1.11", "UniRef:UniRef50_Q9PQV9", "COG:COG0148", "COG:G", "KEGG:K01689", "GO:0000015", "GO:0000287", "GO:0004634", "GO:0005576", "GO:0006096", "GO:0009986" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQV9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQV9" ], "product": "Enolase" } }, "id": "NKIECMJPJD_1220", "locus": "GCA000828735_01217" }, { "type": "cds", "sequence": "contig_1", "start": 704848, "stop": 705111, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATTTTAAATACTTATATTAATCACTATCATTTACCAATAATTAATGATCCACAAGTTGCTTTATTAGATATTAGCAAACACCAAATTAATAATGAAAATTTAACATTTAATCCTTCATACAACTATAATGCCAATCTTTATTTTACAAAAGCAAAATTAATTTATTGTACTTATACAATTATTGGAATAATTTTTATCATTAGTTTTATTTATTATCTAAGTGAAATTATTAAACGATGTTTATTAGTTAATGACAATTGA", "aa": "MILNTYINHYHLPIINDPQVALLDISKHQINNENLTFNPSYNYNANLYFTKAKLIYCTYTIIGIIFIISFIYYLSEIIKRCLLVNDN", "aa_hexdigest": "48e42a5a31d891c2cdac8e1167d29e36", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10277.842599999993, "isoelectric_point": 6.801092338562011 }, "id": "NKIECMJPJD_1221", "locus": "GCA000828735_01218" }, { "type": "cds", "sequence": "contig_1", "start": 705653, "stop": 706573, "strand": "-", "frame": 2, "gene": "gpmI", "product": "2,3-bisphosphoglycerate-independent phosphoglycerate mutase", "db_xrefs": [ "COG:COG0696", "COG:G", "EC:5.4.2.12", "GO:0005829", "GO:0006007", "GO:0006096", "GO:0030145", "GO:0046537", "KEGG:K15633", "SO:0001217", "UniRef:UniRef50_Q9PQW1", "UniRef:UniRef90_Q9PQW1" ], "nt": "ATGTTTGTTAAGCAAGATAAAATATGTGAAAAAACATTTTTAGAGGTTGCACAATCAATCGCAAATTCTTATAAAAGTGATGAATTTGTTCAACCTGTAGTTTTTAATGATGATGAAAAATATAATTTAAAACCACATCAAAAAGTGATTTTAGCAAACTATCGTTCAGATCGTATGCGCCAGTTAGCACACTTACTTAAACCTAACAAAAAATTTCATTACAATAATCCGTTTTTAGTTCAAGATGTTAATTTAATTACTTTAGTACCATTCCCTGATGTTGATGCAATAGCTTTATTTGAGAAGCAAAATTTAAATAACACTTTAGGTGATGTTTTTAATAAACACCAAATAAAAGAAGCTCGAATTGCAGAAACAGAAAAATATGCCCATATATCTTTTTTCTTTGATGGCGGTGTTAATAAATATTATTCGACAAAAACACAATATTTGATTCCTTCGCAAAAAGTTGCTACTTATGATTTATGTCCCCAAATGTCAGCACATTTAATTACGAAAACTATTATCGATCATTATTTCGATCATGATGTTTTTATTGTTAATTATGCAAATCCTGATATGGTTGGTCATAGCGGCAATATGCAGCAAACAATTGAAGCAATTCTAAGTGTTGACCATGAAATTCGAAAATTATATGATTTTTTTGAAAAGAATAATGGTGTGTTGATGATTACAGGTGATCATGGAAATGCTGAAATGATGCTTGATGATCAAAAACAAGTAATTACTTCTCACAGTGTTAATGATGTTTGGTTTATTATCACAGATAATAAAGTTATTTTAAATTCGACACAAAAATTTAGTTTAGCAAATATTGCTCCAACTATTTTGGAATATTTAAATATCGCTCAACCAATTCAAATGACAGCAACGAGTATGATAAAAAAGATTTGTGAATAG", "aa": "MFVKQDKICEKTFLEVAQSIANSYKSDEFVQPVVFNDDEKYNLKPHQKVILANYRSDRMRQLAHLLKPNKKFHYNNPFLVQDVNLITLVPFPDVDAIALFEKQNLNNTLGDVFNKHQIKEARIAETEKYAHISFFFDGGVNKYYSTKTQYLIPSQKVATYDLCPQMSAHLITKTIIDHYFDHDVFIVNYANPDMVGHSGNMQQTIEAILSVDHEIRKLYDFFEKNNGVLMITGDHGNAEMMLDDQKQVITSHSVNDVWFIITDNKVILNSTQKFSLANIAPTILEYLNIAQPIQMTATSMIKKICE", "aa_hexdigest": "0fcd017c36cb6d8f58374e4e92c8ec97", "start_type": "ATG", "rbs_motif": null, "genes": [ "gpmI" ], "truncated": "3-prime", "pseudogene": { "start": 705653, "stop": 706873, "observations": { "mutation": [ 706603, 706789 ], "stop": [ 706603, 706789 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQW1", "query_cov": 1.0, "subject_cov": 0.6095617529880478, "identity": 1.0, "score": 615.0, "evalue": 1.97e-219, "gene_start": 1, "gene_end": 306, "reference_start": 197, "reference_end": 502, "reference_sequence": "MKLNKKIGLIIIDGLGIGKEDNTNAVHLANPPTLNYLINNFPTLQISAAQQNIGLLENQAGNSEIGHLTIGAGRIILNDNANINNYMKSVDYESAVLSNIKNEIVHIVGMYSNGLVHSNYEHIHWIIKQIIKNNNQVVLHLISDGRDDYPYGFAQFIEQINALKKQYNIIVKSLSGRYFAMDRDQRWERTQKAFNAMFVKQDKICEKTFLEVAQSIANSYKSDEFVQPVVFNDDEKYNLKPHQKVILANYRSDRMRQLAHLLKPNKKFHYNNPFLVQDVNLITLVPFPDVDAIALFEKQNLNNTLGDVFNKHQIKEARIAETEKYAHISFFFDGGVNKYYSTKTQYLIPSQKVATYDLCPQMSAHLITKTIIDHYFDHDVFIVNYANPDMVGHSGNMQQTIEAILSVDHEIRKLYDFFEKNNGVLMITGDHGNAEMMLDDQKQVITSHSVNDVWFIITDNKVILNSTQKFSLANIAPTILEYLNIAQPIQMTATSMIKKICE" }, "paralog": false, "identity": 0.9950738916256158, "score": 812.0, "evalue": 8.55e-300, "description": "Internal stop codon at 706603, 706789. Nonsense mutation around 706603, 706789.", "psc": { "uniref90_id": "UniRef90_Q9PQW1", "gene": "gpmI", "product": "2,3-bisphosphoglycerate-independent phosphoglycerate mutase", "ec_ids": [ "5.4.2.12" ], "uniref50_id": "UniRef50_Q9PQW1", "cog_id": "COG0696", "cog_category": "G", "kegg_orthology_id": "K15633", "go_ids": [ "GO:0005829", "GO:0006007", "GO:0006096", "GO:0030145", "GO:0046537" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQW1", "EC:5.4.2.12", "UniRef:UniRef50_Q9PQW1", "COG:COG0696", "COG:G", "KEGG:K15633", "GO:0005829", "GO:0006007", "GO:0006096", "GO:0030145", "GO:0046537" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQW1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQW1" ], "product": "2,3-bisphosphoglycerate-independent phosphoglycerate mutase" } }, "id": "NKIECMJPJD_1222", "locus": "GCA000828735_01219" }, { "type": "cds", "sequence": "contig_1", "start": 706787, "stop": 707161, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATTAAATAAAAAAATAGGTTTGATAATCATTGATGGATTAGGAATTGGAAAAGAAGATAATACTAATGCTGTTCATTTAGCAAATCCACCAACATTAAATTATTTGATTAATAATTTTCCCACATTACAAATTAGTGCGGCTCAACAAAATATTGGTTTACTTGAAAATCAAGCAGGTAACAGCGAAATTGGTCATTTAACAATTGGTGCGGGCCGTATCATTTTAAATGATAATGCAAATATTAATAATTATATGAAATCCGTTGATTATGAAAGTGCAGTTTTAAGCAATATTAAAAATGAAATTGTTCATATTGTTGGCATGTATTCGAATGGTTTAGTACATAGTAATTATGAACATATTCATTGA", "aa": "MKLNKKIGLIIIDGLGIGKEDNTNAVHLANPPTLNYLINNFPTLQISAAQQNIGLLENQAGNSEIGHLTIGAGRIILNDNANINNYMKSVDYESAVLSNIKNEIVHIVGMYSNGLVHSNYEHIH", "aa_hexdigest": "9b27fea752687f9a894c3e261c472dfe", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13529.201999999987, "isoelectric_point": 6.012451362609862 }, "id": "NKIECMJPJD_1223", "locus": "GCA000828735_01220" }, { "type": "cds", "sequence": "contig_1", "start": 707178, "stop": 707366, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00121.24" ], "nt": "ATGATCGAAGGAATTAAAACATATTTATATAATTGTACTGGAATAAATATTCCTATTTTATATGGCGGTAGTGTTAATGACAATAATATTAATGAATTGTGCAATCAAAAATTAATCGATGGTTTTTTAATTGGCAATGCTTCATTAGATGTTAATGTTTTTAATAAAATTATCGATAAATGTAAATAA", "aa": "MIEGIKTYLYNCTGINIPILYGGSVNDNNINELCNQKLIDGFLIGNASLDVNVFNKIIDKCK", "aa_hexdigest": "79463bb1de09f4d01690b994973d8395", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00121.24", "name": "TIM", "length": 57, "aa_cov": 0.8709677419354839, "hmm_cov": 1.0, "evalue": 3.148281041453708e-16, "score": 55.137760162353516, "start": 7, "stop": 60 } ], "seq_stats": { "molecular_weight": 6865.879400000002, "isoelectric_point": 4.927224540710449 }, "id": "NKIECMJPJD_1224", "locus": "GCA000828735_01221" }, { "type": "cds", "sequence": "contig_1", "start": 707412, "stop": 707894, "strand": "-", "frame": 1, "gene": "tpiA", "product": "Triosephosphate isomerase", "db_xrefs": [ "COG:COG0149", "COG:G", "EC:5.3.1.1", "GO:0004807", "GO:0005829", "GO:0006094", "GO:0006096", "GO:0019563", "GO:0046166", "KEGG:K01803", "SO:0001217", "UniRef:UniRef50_Q9PQW2", "UniRef:UniRef90_Q9PQW2" ], "nt": "ATGAAATATATTATTGCTAATTTTAAAATGAATGCAACACAAGAATTAATTAATCATTTTTTAAATAATTTAACGTTATTTGATGAGCAAAAAATTATTATTGGTTTAGCACCAGGGGATTTATATTTAAAAACATTTGTTAATTTAGCTGAAATAAAAAAAGTCAATTTATATGCTCAAAACCCATCATTACATAATAAAGGACCTTATACAGGTCAAATAAGTTGTTTACAATTATTAGATATTAATATTAAAAATGCTTTAGTTGGGCATAGTGAAATACGTATTGATTTTTCACAATCAATAATTGATCAAAAAATAAAAATATCTATGGATTTATTAGAACAAGTCATCATTTGTGTTGGCGAAACTTTTGATGCTTATAAACAAAATAAATCACTTAATTTTGTTTTAAATCAATTAGCCAACATCATTAATTACAAAGGATTAAAAAAAATTATTATCGCTTATGAACCAATTTGA", "aa": "MKYIIANFKMNATQELINHFLNNLTLFDEQKIIIGLAPGDLYLKTFVNLAEIKKVNLYAQNPSLHNKGPYTGQISCLQLLDINIKNALVGHSEIRIDFSQSIIDQKIKISMDLLEQVIICVGETFDAYKQNKSLNFVLNQLANIINYKGLKKIIIAYEPI", "aa_hexdigest": "80e82917041c50e50b5b820b4ee8d13e", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [ "tpiA" ], "truncated": "5-prime", "pseudogene": { "start": 707178, "stop": 707894, "observations": { "mutation": [ 707414 ], "stop": [ 707414 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQW2", "query_cov": 1.0, "subject_cov": 0.6722689075630253, "identity": 1.0, "score": 317.0, "evalue": 3.11e-108, "gene_start": 1, "gene_end": 160, "reference_start": 1, "reference_end": 160, "reference_sequence": "MKYIIANFKMNATQELINHFLNNLTLFDEQKIIIGLAPGDLYLKTFVNLAEIKKVNLYAQNPSLHNKGPYTGQISCLQLLDINIKNALVGHSEIRIDFSQSIIDQKIKISMDLLEQVIICVGETFDAYKQNKSLNFVLNQLANIINYKGLKKIIIAYEPIWAIGTDLELDFKHINYMIEGIKTYLYNCTGINIPILYGGSVNDNNINELCNQKLIDGFLIGNASLDVNVFNKIIDKCK" }, "paralog": false, "identity": 0.9957983193277311, "score": 473.0, "evalue": 3.16e-172, "description": "Internal stop codon at 707414. Nonsense mutation around 707414.", "psc": { "uniref90_id": "UniRef90_Q9PQW2", "gene": "tpiA", "product": "Triosephosphate isomerase", "ec_ids": [ "5.3.1.1" ], "uniref50_id": "UniRef50_Q9PQW2", "cog_id": "COG0149", "cog_category": "G", "kegg_orthology_id": "K01803", "go_ids": [ "GO:0005829", "GO:0004807", "GO:0006094", "GO:0046166", "GO:0019563", "GO:0006096" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQW2", "EC:5.3.1.1", "UniRef:UniRef50_Q9PQW2", "COG:COG0149", "COG:G", "KEGG:K01803", "GO:0005829", "GO:0004807", "GO:0006094", "GO:0046166", "GO:0019563", "GO:0006096" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQW2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQW2" ], "product": "Triosephosphate isomerase" } }, "id": "NKIECMJPJD_1225", "locus": "GCA000828735_01222" }, { "type": "cds", "sequence": "contig_1", "start": 707957, "stop": 708241, "strand": "+", "frame": 2, "gene": "rlmH", "product": "Ribosomal RNA large subunit methyltransferase H", "db_xrefs": [ "COG:COG1576", "COG:J", "EC:2.1.1.177", "GO:0005737", "GO:0070038", "KEGG:K00783", "SO:0001217", "UniRef:UniRef50_Q9PQW3", "UniRef:UniRef90_Q9PQW3" ], "nt": "ATGATGATAAAAATTATAAGTGTAGGGAAATTAAAACAAAAAGCTTTTGTTGATCTAATTAATGATTATTTAAAAAGAATTAACCATTATTTAAAATGCCAAGAAATTGTTGTTAGCGATGAACCTGAACCAGTTCAAATTTCAAATAAATCCTTAGAACAAATTAAAAGTAAAGAGGCCTCTAAAATTTTTAAAAATATTAATCAAAATGATTTTGTAATTGCTTTAATTATTGAAAGCAACATTATAAGTAGCGAAACATTAGCTAAAAAAATACAACAATGA", "aa": "MMIKIISVGKLKQKAFVDLINDYLKRINHYLKCQEIVVSDEPEPVQISNKSLEQIKSKEASKIFKNINQNDFVIALIIESNIISSETLAKKIQQ", "aa_hexdigest": "e623f353c4317df63a7609d96bd27e5d", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "genes": [ "rlmH" ], "truncated": "3-prime", "pseudogene": { "start": 707957, "stop": 708436, "observations": { "mutation": [ 708239 ], "stop": [ 708239 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQW3", "query_cov": 1.0, "subject_cov": 0.5911949685534591, "identity": 1.0, "score": 176.0, "evalue": 8.1e-55, "gene_start": 1, "gene_end": 94, "reference_start": 1, "reference_end": 94, "reference_sequence": "MMIKIISVGKLKQKAFVDLINDYLKRINHYLKCQEIVVSDEPEPVQISNKSLEQIKSKEASKIFKNINQNDFVIALIIESNIISSETLAKKIQQWLNTFSHDICFIIGGSNGLHESIYERANYHLSMSKMTFAHGLAKVMLCEQIYRALSILNNGKYHK" }, "paralog": false, "identity": 0.9937106918238994, "score": 310.0, "evalue": 1.83e-110, "description": "Internal stop codon at 708239. Nonsense mutation around 708239.", "psc": { "uniref90_id": "UniRef90_Q9PQW3", "gene": "rlmH", "product": "Ribosomal RNA large subunit methyltransferase H", "ec_ids": [ "2.1.1.177" ], "uniref50_id": "UniRef50_Q9PQW3", "cog_id": "COG1576", "cog_category": "J", "kegg_orthology_id": "K00783", "go_ids": [ "GO:0005737", "GO:0070038" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQW3", "EC:2.1.1.177", "UniRef:UniRef50_Q9PQW3", "COG:COG1576", "COG:J", "KEGG:K00783", "GO:0005737", "GO:0070038" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQW3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQW3" ], "product": "Ribosomal RNA large subunit methyltransferase H" } }, "id": "NKIECMJPJD_1226", "locus": "GCA000828735_01223" }, { "type": "cds", "sequence": "contig_1", "start": 708438, "stop": 708659, "strand": "-", "frame": 1, "gene": null, "product": "putative protein UU179", "db_xrefs": [ "RefSeq:WP_006688846.1", "SO:0001217", "UniParc:UPI000169E5D8", "UniRef:UniRef100_A0AAC9T327", "UniRef:UniRef50_Q9PQW4", "UniRef:UniRef90_Q9PQW4" ], "nt": "ATGTTAATTAAACCCGCGAAACGCAGTGAAAAAATTTATACAATTATTGCTATAAAAGATAGTGTTGTAACTTTAAAAAATGATTATCAAGACGAAAAAAATATTCAAATTTACGAGTTTGATTTGCAGAATATTATTCCAGAAGTTGGTAAATTTATTAATCTAATTAAATACGATCAACAAGGTTGTTCAGTATTTGAAGAATATGATCAAAACATTTAA", "aa": "MLIKPAKRSEKIYTIIAIKDSVVTLKNDYQDEKNIQIYEFDLQNIIPEVGKFINLIKYDQQGCSVFEEYDQNI", "aa_hexdigest": "f7078d07cf0667b31f11c4f9fae22901", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "ups": { "uniparc_id": "UPI000169E5D8", "ncbi_nrp_id": "WP_006688846.1", "uniref100_id": "UniRef100_A0AAC9T327", "db_xrefs": [ "SO:0001217", "UniParc:UPI000169E5D8", "RefSeq:WP_006688846.1", "UniRef:UniRef100_A0AAC9T327" ] }, "ips": { "uniref100_id": "UniRef100_A0AAC9T327", "uniref90_id": "UniRef90_Q9PQW4" }, "psc": { "uniref90_id": "UniRef90_Q9PQW4", "product": "Uncharacterized protein UU179", "uniref50_id": "UniRef50_Q9PQW4" }, "pscc": { "uniref50_id": "UniRef50_Q9PQW4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQW4" ], "product": "Uncharacterized protein UU179" }, "genes": [], "id": "NKIECMJPJD_1227", "locus": "GCA000828735_01224" }, { "type": "cds", "sequence": "contig_1", "start": 708740, "stop": 709120, "strand": "-", "frame": 2, "gene": "ptsG2", "product": "Putative phosphotransferase enzyme IIB component UU178", "db_xrefs": [ "COG:COG1264", "COG:G", "EC:2.7.1.-", "GO:0008982", "GO:0009401", "GO:0016020", "RefSeq:WP_006688859.1", "SO:0001217", "UniParc:UPI00001393EA", "UniRef:UniRef100_Q9PQW5", "UniRef:UniRef50_Q9PQW5", "UniRef:UniRef90_Q9PQW5" ], "nt": "ATGCAAAATTTAAATAATAAGCAAAAAACATTAATTATTTTTTTAGGTATTATTACCTTTGGAATTTTTATTATTTACTTTTTTACAAAAGCTAAAAAAGTATCTCAAATTAGAAACACTCATTTAATAGTATCTTCAAATATTCCTTTTAGTTTAAATGATTTTTATAATTGTATTGGTTCAAAAAATAATTTAGTTAACGTTGATGCGACAATTAATACATTAAAAATTGAATTAAAAGAAATTAACGCATTGAATAATGAAAAATTAAAGGTTTTAGGTGCAAAAGGGATTATGTGCAATCAAACAAAAATTTCTATTATTTTTGGTGATTTTTGTTTGGAACTTAAAGAATTAATTAAAAAAGATTTATTTTCATAG", "aa": "MQNLNNKQKTLIIFLGIITFGIFIIYFFTKAKKVSQIRNTHLIVSSNIPFSLNDFYNCIGSKNNLVNVDATINTLKIELKEINALNNEKLKVLGAKGIMCNQTKISIIFGDFCLELKELIKKDLFS", "aa_hexdigest": "9230aada5e140e9b7cbbd6e4e87bf9b1", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI00001393EA", "ncbi_nrp_id": "WP_006688859.1", "uniref100_id": "UniRef100_Q9PQW5", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001393EA", "RefSeq:WP_006688859.1", "UniRef:UniRef100_Q9PQW5" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQW5", "uniref90_id": "UniRef90_Q9PQW5" }, "psc": { "uniref90_id": "UniRef90_Q9PQW5", "gene": "PtsG2", "product": "Putative phosphotransferase enzyme IIB component UU178", "ec_ids": [ "2.7.1.-" ], "uniref50_id": "UniRef50_Q9PQW5", "cog_id": "COG1264", "cog_category": "G", "go_ids": [ "GO:0016020", "GO:0008982", "GO:0009401" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQW5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQW5" ], "product": "Putative phosphotransferase enzyme IIB component UU178" }, "genes": [ "ptsG2" ], "id": "NKIECMJPJD_1228", "locus": "GCA000828735_01225" }, { "type": "cds", "sequence": "contig_1", "start": 709123, "stop": 709626, "strand": "-", "frame": 3, "gene": "nadK", "product": "NAD kinase", "db_xrefs": [ "COG:COG0061", "COG:H", "EC:2.7.1.23", "GO:0003951", "GO:0005524", "GO:0005737", "GO:0006741", "GO:0019674", "GO:0046872", "GO:0051287", "KEGG:K00858", "SO:0001217", "UniRef:UniRef50_Q9PQW6", "UniRef:UniRef90_Q9PQW6" ], "nt": "ATGTATTATAATCCTTTATTACTTAAAGTAAGTATTAATAACCAAAATTTTTTTTATTGTTTGAATGAGTTAAGTTTATTTTCTAATGAGTTAGTGTCTTTTGATATTAGCATAAATGATTATCCATATGAAAAATTTCGTGGTTCTGGTTTGTTATTTGTAACACCAAGCGGATCAACTGGCAAAAACAAAACAGCTTTTGGTCCCATTATTTTTAATAATCATGAGAATTTTATTATGACAGAGATTTTTCCTGTTAATCATTTAAAATATTCAAGTTTAAATGCCCCTGTGGTTTTTAGAAAAGATTATAAAATCAGTTTAACAAATATTAAATTTAAAAAATCTTTTAGTGTTGCTATTGATGGGAATATCATTAATTTTAGCGATAAAATTAATGATATTAAAGTAGAAACAATCCAAGCTTCATCAAAAATTCATGGTTTAAATAATTTTAAGAAATACATTGATAAATTAAACAAATCTTTTATTAAAGGAGAATAA", "aa": "MYYNPLLLKVSINNQNFFYCLNELSLFSNELVSFDISINDYPYEKFRGSGLLFVTPSGSTGKNKTAFGPIIFNNHENFIMTEIFPVNHLKYSSLNAPVVFRKDYKISLTNIKFKKSFSVAIDGNIINFSDKINDIKVETIQASSKIHGLNNFKKYIDKLNKSFIKGE", "aa_hexdigest": "52772a0a3d2ac2f0fe0dd98d9065f35d", "start_type": "ATG", "rbs_motif": null, "genes": [ "nadK" ], "truncated": "3-prime", "pseudogene": { "start": 709123, "stop": 709926, "observations": { "mutation": [ 709734 ], "stop": [ 709734 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQW6", "query_cov": 1.0, "subject_cov": 0.6185185185185185, "identity": 1.0, "score": 331.0, "evalue": 2.04e-113, "gene_start": 1, "gene_end": 167, "reference_start": 104, "reference_end": 270, "reference_sequence": "MKNNKPICFYDIYCFKPQECFKRNDINLLEDKLKRYSKITFLRNKENPEIIFLLGGDGSFINFINQQWKKNVKIVGINYGQLGFYSSYDSIKTINLDEIIDENMYYNPLLLKVSINNQNFFYCLNELSLFSNELVSFDISINDYPYEKFRGSGLLFVTPSGSTGKNKTAFGPIIFNNHENFIMTEIFPVNHLKYSSLNAPVVFRKDYKISLTNIKFKKSFSVAIDGNIINFSDKINDIKVETIQASSKIHGLNNFKKYIDKLNKSFIKGE" }, "paralog": false, "identity": 0.9962546816479401, "score": 540.0, "evalue": 4.26e-198, "description": "Internal stop codon at 709734. Nonsense mutation around 709734.", "psc": { "uniref90_id": "UniRef90_Q9PQW6", "gene": "nadK", "product": "NAD kinase", "ec_ids": [ "2.7.1.23" ], "uniref50_id": "UniRef50_Q9PQW6", "cog_id": "COG0061", "cog_category": "H", "kegg_orthology_id": "K00858", "go_ids": [ "GO:0005737", "GO:0005524", "GO:0046872", "GO:0051287", "GO:0003951", "GO:0019674", "GO:0006741" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQW6", "EC:2.7.1.23", "UniRef:UniRef50_Q9PQW6", "COG:COG0061", "COG:H", "KEGG:K00858", "GO:0005737", "GO:0005524", "GO:0046872", "GO:0051287", "GO:0003951", "GO:0019674", "GO:0006741" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQW6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQW6" ], "product": "NAD kinase" } }, "id": "NKIECMJPJD_1229", "locus": "GCA000828735_01226" }, { "type": "cds", "sequence": "contig_1", "start": 709732, "stop": 709935, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAATAATAAGCCAATTTGTTTTTATGATATCTATTGTTTTAAACCACAAGAATGTTTTAAAAGAAATGATATTAATTTGTTAGAAGATAAACTAAAAAGATATTCAAAAATAACATTTTTACGAAATAAAGAAAATCCTGAAATTATTTTTTTATTAGGTGGAGATGGATCGTTTATCAATTTTATTAACCAACAATGA", "aa": "MKNNKPICFYDIYCFKPQECFKRNDINLLEDKLKRYSKITFLRNKENPEIIFLLGGDGSFINFINQQ", "aa_hexdigest": "f353284c2e324bd4b08f7fbbd622573d", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8068.2900999999965, "isoelectric_point": 8.8624849319458 }, "id": "NKIECMJPJD_1230", "locus": "GCA000828735_01227" }, { "type": "cds", "sequence": "contig_1", "start": 709925, "stop": 710311, "strand": "-", "frame": 2, "gene": "arsC", "product": "putative protein UU176", "db_xrefs": [ "COG:COG1393", "COG:P", "GO:0016491", "KEGG:K16509", "RefSeq:WP_006688944.1", "SO:0001217", "UniParc:UPI00001393E2", "UniRef:UniRef100_Q9PQW7", "UniRef:UniRef50_Q9PQW7", "UniRef:UniRef90_Q9PQW7" ], "nt": "ATGATTTACGTTTTATACAGTCCTAATTGCGCAGTGTGTAAAAAAGTTGTGCGATTTTTCCGAAATAATCAAATTGAAATTACCAAAATTATTATTGGTGAAGATAAAATAGAACGTTCTATGTTGTTAGATATTTTATCTCTTTGTGAAGATGGCTTTGGAACAATTATATCTTTTAAGACAGAATCTTCAAAACGCTTGAATATTACTTCAAAAACTTTTTTAGATTTATCTACAAAAGAATTATTAAATTTAATTCAAAATGATTTAAATTTAATTCGTCGCCCTTTAATCTATCAAACAAAAAACAATCGACCATATCGTTTACAAATTGGTTATGATTCAGAAGAAATTGAAATTTTTAAACGAGCAGTGCATGAAAAATAA", "aa": "MIYVLYSPNCAVCKKVVRFFRNNQIEITKIIIGEDKIERSMLLDILSLCEDGFGTIISFKTESSKRLNITSKTFLDLSTKELLNLIQNDLNLIRRPLIYQTKNNRPYRLQIGYDSEEIEIFKRAVHEK", "aa_hexdigest": "f0967a88c83258b88b7d3528daa4db81", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001393E2", "ncbi_nrp_id": "WP_006688944.1", "uniref100_id": "UniRef100_Q9PQW7", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001393E2", "RefSeq:WP_006688944.1", "UniRef:UniRef100_Q9PQW7" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQW7", "uniref90_id": "UniRef90_Q9PQW7" }, "psc": { "uniref90_id": "UniRef90_Q9PQW7", "gene": "ArsC", "product": "Uncharacterized protein UU176", "uniref50_id": "UniRef50_Q9PQW7", "cog_id": "COG1393", "cog_category": "P", "kegg_orthology_id": "K16509", "go_ids": [ "GO:0016491" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQW7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQW7" ], "product": "Uncharacterized protein UU176" }, "genes": [ "arsC" ], "id": "NKIECMJPJD_1231", "locus": "GCA000828735_01228" }, { "type": "cds", "sequence": "contig_1", "start": 710436, "stop": 711329, "strand": "+", "frame": 3, "gene": "trpS", "product": "tryptophan--tRNA ligase", "db_xrefs": [ "COG:COG0180", "COG:J", "EC:6.1.1.2", "GO:0000166", "GO:0004830", "GO:0005524", "GO:0005829", "GO:0006436", "SO:0001217", "UniRef:UniRef50_Q9PQW8", "UniRef:UniRef90_Q9PQW8" ], "nt": "ATGAAAAGACTAATTTCAGGAATTCAACCAACTAATAATTTAACATTAGGAAATTATTTAGGTGCAATTAAAAACTTTGTTGATTTACAAGATGATTATGAAGTGTTTTTATTTGTTGCGGATTTACATTCCTTAACACCAAATATTTTTGATAACACTGATTTTTCTACTACTAAACGTCAGATTATCGCGACATATTTAGCTGCTGGTATTGATCCTAAAAAAACATGTCTATTTTACCAATCAGATATCTTATCGATTCCATTATTAGCTCACATATTATTATGTTCAACAAGTATTGGTGAACTAACGCGTATGACACAATTTAAAGATAAAAGTATTAAAGCAACAAAGATGGCTAATAACACTGAAATGATTCCTTCTGGTTTATTAACATATCCCACATTAATGGCAGCAGATATTTTAACTTTTAATGCAGATGTTGTTCCTGTTGGCCAAGATCAAAAACAACATTTGGAATTAACAAGAACGCTTGCTGACCGTTTTAATAAAAGATATGGTAATACTTTTAAATTGCCTCAAGTTTATATTCCTAAAATAGGGGCAAAAATTATGGATTTATTAGATCCTAATATTAAAATGTCAAAATCTTCTAAAAACCTTAAGGGTGTGATTTTTTTAAATGATTCAAAGGATATAATTTTTAAAAAAATTAAAGGAGCTTTAACAGATAATTTAAACAAAGTTAAATATGATTTAACATTACAACCAGGAGTGAGTAATTTAATGACAATTTATGCTTGTTTAACAAATCTAAGCTTTAAAGAAATCGAACTAAAATACCAACAACAAAACTATGGTGTTTTTAAAAATGATTTAGCCAATATCGTTGCTGATTTTTTAGAAAAGCTGCAACAAAAAATTTCTTATTGA", "aa": "MKRLISGIQPTNNLTLGNYLGAIKNFVDLQDDYEVFLFVADLHSLTPNIFDNTDFSTTKRQIIATYLAAGIDPKKTCLFYQSDILSIPLLAHILLCSTSIGELTRMTQFKDKSIKATKMANNTEMIPSGLLTYPTLMAADILTFNADVVPVGQDQKQHLELTRTLADRFNKRYGNTFKLPQVYIPKIGAKIMDLLDPNIKMSKSSKNLKGVIFLNDSKDIIFKKIKGALTDNLNKVKYDLTLQPGVSNLMTIYACLTNLSFKEIELKYQQQNYGVFKNDLANIVADFLEKLQQKISY", "aa_hexdigest": "84a863593e94bca8f8d47bc79b66d7bb", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "psc": { "uniref90_id": "UniRef90_Q9PQW8", "query_cov": 1.0, "subject_cov": 0.8918918918918919, "identity": 1.0, "score": 584.0, "evalue": 3.15e-210, "valid": true, "gene": "trpS", "product": "tryptophan--tRNA ligase", "ec_ids": [ "6.1.1.2" ], "uniref50_id": "UniRef50_Q9PQW8", "cog_id": "COG0180", "cog_category": "J", "go_ids": [ "GO:0000166", "GO:0004830", "GO:0005524", "GO:0005829", "GO:0006436" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQW8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQW8" ], "product": "Tryptophan--tRNA ligase" }, "genes": [ "trpS" ], "id": "NKIECMJPJD_1232", "locus": "GCA000828735_01229" }, { "type": "cds", "sequence": "contig_1", "start": 711463, "stop": 711852, "strand": "+", "frame": 1, "gene": null, "product": "HAD-IIB family hydrolase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0022B758C4", "UniRef:UniRef90_UPI0022B758C4" ], "nt": "ATGGGAAAAAACAACAAATATTTAATTGCTTGTGATCTAGATGGAACACTTTTAAATAATGATAGTGATATATCAGAAAAAACAATTGAGGGAATTAAAAAAATCATCAATCAAGGCCATATTTTCTGTATTGTAACAGGTCGTCCCTTACGTGGATCAATCCATATTTATGAAAAATTAGGATTAACAACTTTAATGGCTAATTATAATGGCTCTTATATTTCAAACCCTTCCGATAAAAAATTCGCGCCAATTAATATGACATTTAGTAAAGATATTGTAAGCGCAATTTTAAAAAATCGATTTATTGCTAAAAATATTGTTAATGCTGTTGTTGAAGCTGATAATGTTGCTACCATTTTAAACAGTAATATGGATTTAAATATTTGA", "aa": "MGKNNKYLIACDLDGTLLNNDSDISEKTIEGIKKIINQGHIFCIVTGRPLRGSIHIYEKLGLTTLMANYNGSYISNPSDKKFAPINMTFSKDIVSAILKNRFIAKNIVNAVVEADNVATILNSNMDLNI", "aa_hexdigest": "e941781da234dd461985c9f43d8a929b", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 711463, "stop": 711967, "observations": { "insertion": [ 711901 ], "mutation": [ 711850 ], "stop": [ 711850, 711911 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0022B758C4", "query_cov": 1.0, "subject_cov": 0.7724550898203593, "identity": 1.0, "score": 251.0, "evalue": 4.32e-84, "gene_start": 1, "gene_end": 129, "reference_start": 1, "reference_end": 129, "reference_sequence": "MGKNNKYLIACDLDGTLLNNDSDISEKTIEGIKKIINQGHIFCIVTGRPLRGSIHIYEKLGLTTLMANYNGSYISNPSDKKFAPINMTFSKDIVSAILKNRFIAKNIVNAVVEADNVATILNSNMDLNIWKSFIDIFHVPIRDMNEKINWLRKMILTTLLEMLMPFY" }, "paralog": false, "identity": 0.9880239520958084, "score": 319.0, "evalue": 2.76e-113, "description": "Internal stop codon at 711850, 711911. Frameshift due to insertion around 711901. Nonsense mutation around 711850.", "psc": { "uniref90_id": "UniRef90_UPI0022B758C4", "product": "HAD-IIB family hydrolase", "uniref50_id": "UniRef50_UPI0022B758C4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0022B758C4", "UniRef:UniRef50_UPI0022B758C4" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0022B758C4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0022B758C4" ], "product": "HAD-IIB family hydrolase" } }, "id": "NKIECMJPJD_1233", "locus": "GCA000828735_01230" }, { "type": "cds", "sequence": "contig_1", "start": 711892, "stop": 712047, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGAAAAAAATAAATTGACTAAGGAAAATGATATTAACAACCTTATTAGAGATGCTAATGCCCTTTTATTAAATGTCAACGATAAAGCTTTATTTGATTCTATGGTTTATCATATTAAACAAATTGCTCCCACTTTAGCGGTTCGTAGCTGA", "aa": "MNEKNKLTKENDINNLIRDANALLLNVNDKALFDSMVYHIKQIAPTLAVRS", "aa_hexdigest": "34895fd00a13c4de98010f5009120ae9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5811.6478, "isoelectric_point": 8.120453834533691 }, "id": "NKIECMJPJD_1234", "locus": "GCA000828735_01231" }, { "type": "cds", "sequence": "contig_1", "start": 712111, "stop": 712305, "strand": "+", "frame": 1, "gene": null, "product": "HAD hydrolase family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0022B7618D", "UniRef:UniRef90_UPI0022B7618D" ], "nt": "ATGGCTATGCGCTATTTATCCTCATATTATGGAATTCCATTAGATCGTTGTATAGCTTTCGGCGATGGTGAAAATGATTTGACAATGCTAAACCATGCTGGATATGGCTTTGCCATGAAAAATGGTACACGTCCCGCTAAATTACTAGCACGTCACATTACTAAACACACAAACGTAGAAAATGGTGTTGTTTGA", "aa": "MAMRYLSSYYGIPLDRCIAFGDGENDLTMLNHAGYGFAMKNGTRPAKLLARHITKHTNVENGVV", "aa_hexdigest": "e1884759a314259f1fb411fecff262b6", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 711910, "stop": 712338, "observations": { "mutation": [ 712045, 712303 ], "stop": [ 712045, 712303 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0022B7618D", "query_cov": 1.0, "subject_cov": 0.4507042253521127, "identity": 1.0, "score": 136.0, "evalue": 6.51e-40, "gene_start": 1, "gene_end": 64, "reference_start": 68, "reference_end": 131, "reference_sequence": "MTKENDINNLIRDANALLLNVNDKALFDSMVYHIKQIAPTLAVRSWSARVDDQFEVIEINSSFADKAMAMRYLSSYYGIPLDRCIAFGDGENDLTMLNHAGYGFAMKNGTRPAKLLARHITKHTNVENGVVWELEYVLNENK" }, "paralog": false, "identity": 0.9788732394366197, "score": 276.0, "evalue": 1.16e-97, "description": "Internal stop codon at 712045, 712303. Nonsense mutation around 712045, 712303.", "psc": { "uniref90_id": "UniRef90_UPI0022B7618D", "product": "HAD hydrolase family protein", "uniref50_id": "UniRef50_UPI0022B7618D", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0022B7618D", "UniRef:UniRef50_UPI0022B7618D" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0022B7618D", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0022B7618D" ], "product": "HAD hydrolase family protein" } }, "id": "NKIECMJPJD_1235", "locus": "GCA000828735_01232" }, { "type": "cds", "sequence": "contig_1", "start": 712414, "stop": 713574, "strand": "+", "frame": 1, "gene": null, "product": "putative protein UU173", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQX0", "UniRef:UniRef90_Q9PQX0" ], "nt": "ATGCGCACAGGGAAATACATTACTAAATATGATTACATTGGTTATTATACAAAGCAAAAAGCAATGTGGTTTTTCACTAATGCTGAGATTAAAGCTAAAATCGACTTATTGTTAAAAAATGTTAGTTCTTATGATTTTGAGGATTTAGATGATGAAGAAGAACATGATTATGAAATTGATGCATATGAAATTTATAAGGAACAAACTGATTTTCTTTCACAATTAGAAATTAATAAAAATATAGATGTGGACAATCCTAAAATTGCTGAAGGTATTTTATTAGATAAAGCATCACAAGAAGATATTAAAAAAACTTATCATTACATTAAAAAAATTATTAATTTCGAAACTGATCCTTTATTTAAAAACAAATCATTAGAAGAGCTTGCTAATTTAACTTTAGAATTAATTGATAAAAATGATAAAATCATTTTTTTTCAACCTGTTTTTATTAATGATAAATTAATTACTAAACCCGATTGTTTTATCAAAAAGAACGATGAATTTATTGTTATCGAAACAAAAGGGACAAGTTCAATTAAAAAACACCATATTTTAGATGTATTTTACCAAGCACAAGTTATCTCAAAACACCCTTATTTTATAAATAAATGTATAAGTTATCAAATGTGCATTGTTGATTATGTTAAATTAAAAAAAAATCAAGTAAATTTTACAATTTCTCCTTATTATAATTATCAAAAAAGCGTAGTTTTATCTTTACCACCAAGTGCTGCAAATCAATTTAATAAATTTGAAATTAATGAATTAAAACGTAAACGTAAGCGTGGAGAAGCAATTTATTTAAAAAAAGACGAAAATAAAAAATATTATGAAGATATTGATGCTTATGATCATTGTAAAGGTATTTTAATAGATGATTTAATCGATGGAACCTATAGTAGCATAAATGATTATAAGGAAGCCAAAAAAATTTATGATTACAAAACAGAAGATTTAAAAGATGATGTACGTTTTAATAAAACCATGGATGAATTAATTAAAGGCATTGAAAAATTACACAATGATTTTGATGAAGTAATCAGCGAATTATTAGTTCATAAACAAAATTTATTAGCTTTGCCTATCGAACAAAGAATTCCTAACTATTTTATACCATCATATAATGATAAGGGTGACTATAAGGATAATGATTTTTGA", "aa": "MRTGKYITKYDYIGYYTKQKAMWFFTNAEIKAKIDLLLKNVSSYDFEDLDDEEEHDYEIDAYEIYKEQTDFLSQLEINKNIDVDNPKIAEGILLDKASQEDIKKTYHYIKKIINFETDPLFKNKSLEELANLTLELIDKNDKIIFFQPVFINDKLITKPDCFIKKNDEFIVIETKGTSSIKKHHILDVFYQAQVISKHPYFINKCISYQMCIVDYVKLKKNQVNFTISPYYNYQKSVVLSLPPSAANQFNKFEINELKRKRKRGEAIYLKKDENKKYYEDIDAYDHCKGILIDDLIDGTYSSINDYKEAKKIYDYKTEDLKDDVRFNKTMDELIKGIEKLHNDFDEVISELLVHKQNLLALPIEQRIPNYFIPSYNDKGDYKDNDF", "aa_hexdigest": "bdca69183c29de5658b716351c03283e", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 712414, "stop": 713877, "observations": { "mutation": [ 713572 ], "stop": [ 713572 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQX0", "query_cov": 1.0, "subject_cov": 0.5594202898550724, "identity": 1.0, "score": 769.0, "evalue": 4.09e-276, "gene_start": 1, "gene_end": 386, "reference_start": 1, "reference_end": 386, "reference_sequence": "MRTGKYITKYDYIGYYTKQKAMWFFTNAEIKAKIDLLLKNVSSYDFEDLDDEEEHDYEIDAYEIYKEQTDFLSQLEINKNIDVDNPKIAEGILLDKASQEDIKKTYHYIKKIINFETDPLFKNKSLEELANLTLELIDKNDKIIFFQPVFINDKLITKPDCFIKKNDEFIVIETKGTSSIKKHHILDVFYQAQVISKHPYFINKCISYQMCIVDYVKLKKNQVNFTISPYYNYQKSVVLSLPPSAANQFNKFEINELKRKRKRGEAIYLKKDENKKYYEDIDAYDHCKGILIDDLIDGTYSSINDYKEAKKIYDYKTEDLKDDVRFNKTMDELIKGIEKLHNDFDEVISELLVHKQNLLALPIEQRIPNYFIPSYNDKGDYKDNDFWLELRNLYAYSGYDVIKYSGKILTIKKFGLDNVTKDILGIDILKTYANQTFFNLYKNNQIKIYQAAVDLFAKLKSKKVYFDFESINSAIRAVDNSFPFTQTVTQNSIIIDDGSCDIKNLKCTNMIIDPREINNEWFKAIIDKIHQGLEYSYIVYNKSFEASRLKEMAEFINEEIYELKVSEIIKNMYDLADFFIVSMGKQLIVIPELKGFYSIKKVLPIIEKEFPQIYHDVNCLNYKELSVGNGLVCQTKTTKRFFNTITDLEWEQFVHDARIYCENDVRAMIAVEYFIKKLIDEAKMKNLILS" }, "paralog": false, "identity": 0.997946611909651, "score": 981.0, "evalue": 0.0, "description": "Internal stop codon at 713572. Nonsense mutation around 713572.", "psc": { "uniref90_id": "UniRef90_Q9PQX0", "product": "Uncharacterized protein UU173", "uniref50_id": "UniRef50_Q9PQX0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQX0", "UniRef:UniRef50_Q9PQX0" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQX0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQX0" ], "product": "Uncharacterized protein UU173" } }, "id": "NKIECMJPJD_1236", "locus": "GCA000828735_01233" }, { "type": "cds", "sequence": "contig_1", "start": 713788, "stop": 714363, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF11074.14" ], "nt": "TTGAAATCAAAAAAGGTTTATTTTGATTTTGAATCAATTAATTCAGCAATTCGCGCTGTTGATAATTCGTTTCCTTTTACCCAAACTGTTACACAAAATTCTATTATTATTGATGATGGAAGTTGTGATATTAAAAATTTAAAATGCACTAATATGATTATTGATCCACGTGAAATTAATAATGAATGGTTTAAAGCAATTATTGATAAAATTCACCAGGGATTAGAATATTCATATATTGTATACAACAAATCTTTTGAAGCTTCGCGTCTTAAAGAAATGGCTGAATTCATTAATGAAGAAATATATGAATTAAAGGTTAGCGAAATAATTAAAAACATGTATGATTTAGCTGATTTTTTTATAGTTTCAATGGGAAAGCAATTAATTGTAATTCCTGAATTAAAAGGTTTTTATTCAATTAAAAAAGTCTTACCAATTATTGAAAAAGAATTTCCACAAATTTATCACGATGTTAATTGTTTAAACTACAAAGAACTAAGTGTGGGTAATGGTTTGGTTTGTCAAACAAAAACAACAAAACGTTTTTTCAATACAATTACTGATCTAGAATGA", "aa": "MKSKKVYFDFESINSAIRAVDNSFPFTQTVTQNSIIIDDGSCDIKNLKCTNMIIDPREINNEWFKAIIDKIHQGLEYSYIVYNKSFEASRLKEMAEFINEEIYELKVSEIIKNMYDLADFFIVSMGKQLIVIPELKGFYSIKKVLPIIEKEFPQIYHDVNCLNYKELSVGNGLVCQTKTTKRFFNTITDLE", "aa_hexdigest": "79acd12e0cbd08db906a9b7dc879c14c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF11074.14", "name": "DUF2779", "length": 137, "aa_cov": 0.7068062827225131, "hmm_cov": 0.9197080291970803, "evalue": 9.137063722510684e-29, "score": 95.87157440185547, "start": 6, "stop": 140 } ], "seq_stats": { "molecular_weight": 22260.45050000002, "isoelectric_point": 5.369034004211425 }, "id": "NKIECMJPJD_1237", "locus": "GCA000828735_01234" }, { "type": "cds", "sequence": "contig_1", "start": 714541, "stop": 714780, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0026EF6BA9" ], "nt": "GTGTTTACTAAGGTATTTAATTTAGATAAAGAACAAAAATATCATATTAACAATGTAATGTTCAGCCATAAATTAATTCCCAATTTATTGAAAGCATTGGATATAAAAAACAATACAACTAATGCCGAATTAATTAAAAAAATTAAAGAAAATATGACTGCTATTGATGAAAAAACACAAACAAATTTAAATACAAATTACATTCCTTTGTCACTTACAGAAACAAATAATAAAAAATAA", "aa": "MFTKVFNLDKEQKYHINNVMFSHKLIPNLLKALDIKNNTTNAELIKKIKENMTAIDEKTQTNLNTNYIPLSLTETNNKK", "aa_hexdigest": "f949b614e53f7f68e89221192ae07cb0", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0026EF6BA9", "query_cov": 0.9746835443037974, "subject_cov": 1.0, "identity": 0.74, "score": 105.0, "evalue": 1.95e-28, "valid": false, "uniref50_id": "UniRef50_UPI0026EF6BA9" }, "pscc": { "uniref50_id": "UniRef50_UPI0026EF6BA9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0026EF6BA9" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9222.579099999992, "isoelectric_point": 9.436511421203612 }, "id": "NKIECMJPJD_1238", "locus": "GCA000828735_01235" }, { "type": "cds", "sequence": "contig_1", "start": 715018, "stop": 715302, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTAGCAGAAGATAATAATGAAACTAAAAATAAAGCTTTAGAATTATTTAAAGATTACTTTACAACAATCATGAAAGCAACAAAAGTAAATGATAAAGTTCATTATGATCCAAGTTTTATTAATGAATTTTATGGTTCAATAAGCAATTTGTTTGAAGAGCATGATTTAGAGCCATCAGTGGAAATTGAATCTAATAAAATCCGTATTGTTAAAGAAGATAAAGATGTAATAAAAGACAAAACAGTTACTGCAACATTTAATTTATACAATGGCTTTGACTGA", "aa": "MLAEDNNETKNKALELFKDYFTTIMKATKVNDKVHYDPSFINEFYGSISNLFEEHDLEPSVEIESNKIRIVKEDKDVIKDKTVTATFNLYNGFD", "aa_hexdigest": "848cd1d2dea6d3e72bd1e49649befdab", "start_type": "TTG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10959.119499999986, "isoelectric_point": 4.762050437927246 }, "id": "NKIECMJPJD_1239", "locus": "GCA000828735_01236" }, { "type": "cds", "sequence": "contig_1", "start": 715303, "stop": 716007, "strand": "-", "frame": 3, "gene": null, "product": "MBA N-terminal paralog", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B5ZAX8", "UniRef:UniRef90_D2EDJ8" ], "nt": "ATGTCATTAAGTGCGATTGCAATTAGTACAAGTATAGTTGCGTTCGCAGCTTCTTGTTCAAATACAAATATTAAATCTAAGTTAAGCAAGCAATTAGCACAAACAAAAGATCAAAAAAACCATTTTGCTGTTTATGAAATTGAAAATTATACTAAACTAACAGATGAAGAAAAGAAGTCATTAGAAAATCTTGAGTTTGGTGTTTCTATTTTGGATAAAGATGGTAAAAATCCAATAAATATTAAAGTTAAAGGAGTTCAAAAAGAAGGTAAGATTTACGTTAAATTGCCACGCAAACCAGAAGTAAATGAAAAATTACTTGTTGTACCAACTAAAAATATTTTAAAAACAGAAGCATTAGTTTTAAATAATGATAATTTAAATTATCAAACAGTAACTTTTGAACAAGTTCCTGATCAAAAATTAGAACCAAAAGAAGAAAAACAAACTAAAGCTGTTGATTATGAAAAAGCAATTAAAGATGAAGATTATGTAAAACAAATTACATTATCTAATTTAGAAAAATATATTAGTAATGAGTATCAATATTTAAATCCAAATAATAAAACACTAGATTATAAAAATCATAGTTTTGTTCAAAAGTTTTTAGACGAAATTGATCTTTTAGCACAAAAATTAGAAAGCAACGACCTTAATTTAGAAGATAAACAAATTTATGAAAAAATTATTTTAGGATTACGTTGA", "aa": "MSLSAIAISTSIVAFAASCSNTNIKSKLSKQLAQTKDQKNHFAVYEIENYTKLTDEEKKSLENLEFGVSILDKDGKNPINIKVKGVQKEGKIYVKLPRKPEVNEKLLVVPTKNILKTEALVLNNDNLNYQTVTFEQVPDQKLEPKEEKQTKAVDYEKAIKDEDYVKQITLSNLEKYISNEYQYLNPNNKTLDYKNHSFVQKFLDEIDLLAQKLESNDLNLEDKQIYEKIILGLR", "aa_hexdigest": "ed685e27652e16e1d5d634009fadd546", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 715000, "stop": 716040, "observations": { "mutation": [ 715020, 715305, 716013 ], "stop": [ 715020, 715305, 716013 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D2EDJ8", "query_cov": 1.0, "subject_cov": 0.6206896551724138, "identity": 0.996, "score": 433.0, "evalue": 5.93e-151, "gene_start": 1, "gene_end": 234, "reference_start": 12, "reference_end": 245, "reference_sequence": "MKLLKNKKFWAMSLSAIAISTSIVAFAASCSNTNIKSKLSKQLAQTKDQKNHFAVYEIENYTKLTDEEKKSLENLEFGVSILDKDGKNPINIKVKGVQKEGKIYVKLPRKPEVNEKLLVVPTKNILKTEALVLNNDNLNYQTATFEQVPDQKLEPKEEKQTKAVDYEKAIKDEDYVKQITLSNLEKYISNEYQYLNPNNKTLDYKNHSFVQKFLDEIDLLAQKLESNDLNLEDKQIYEKIILGLRWLLAEDNNETKNKALELFKDYFTTIMKATKVNDKVHYDPSFINEFYGSISNLFEEHDLEPSVEIESNKIRIVKEDKDVIKDKTVTATFNLYNGFDWSTLLKDVVFKEYKKDSNGKPVIDEKTKMPIETDKIV" }, "paralog": false, "identity": 0.9884393063583815, "score": 666.0, "evalue": 2.43e-245, "description": "Internal stop codon at 715020, 715305, 716013. Nonsense mutation around 715020, 715305, 716013.", "psc": { "uniref90_id": "UniRef90_D2EDJ8", "product": "MBA N-terminal paralog", "uniref50_id": "UniRef50_B5ZAX8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D2EDJ8", "UniRef:UniRef50_B5ZAX8" ] }, "pscc": { "uniref50_id": "UniRef50_B5ZAX8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B5ZAX8" ], "product": "Lipoprotein" } }, "id": "NKIECMJPJD_1240", "locus": "GCA000828735_01237" }, { "type": "cds", "sequence": "contig_1", "start": 716415, "stop": 717044, "strand": "+", "frame": 3, "gene": null, "product": "DUF1410 domain-containing protein", "db_xrefs": [ "RefSeq:WP_231845357.1", "SO:0001217", "UniParc:UPI000ABC946E", "UniRef:UniRef100_UPI00016C0373", "UniRef:UniRef50_A0AA86Z492", "UniRef:UniRef90_A0AA86Z492" ], "nt": "GTGATTGCAAATAAAATAACAATTAATAAAATTCAAAACGGCACTGCTACTATTGAAGTTACATTTAGTAAATTTAAATTAGCAGATGCTAATAAAAAAGATTTTGTTTTAGAAGTTAAAGATAGTGCTAATGATGACGCTTCTGCTATTAGTGCTACTGAATTAGTATTTGATGCAAATAAAAAAATACTTACAGGTAAATTAAATGGCTTAGCATCTAATATTAACTATAAAATATCAAAATTTACTTTAAATAATAATGAAATTAAATTTGATTTTAAACAAGAAGACCAACAAGAATTATTAAACCAATATATTCAACAAGCAGAATTAAATACAATAATTGATAAAGCTAATAAAAAAATTAACATTAAATTACAAAATTTTAGTATATTAAATTCTTTTCAAGATAATCAACCTGTATTAACATTTGATATTGAAATTAATAAAAAAGACGGAATAACACAAGTTGTCCATAAATCGTTAACAAAGAATCAATTATTAAATCCTAATGGTTTGGAAATTGATTTAAAAGATAAAATGAAAAATAATATTGATTATGATGTTAAATTGACTAATGTTAAAATTGCAAATATTGCATTAAATAATGATAATGTTAATAATATTTAA", "aa": "MIANKITINKIQNGTATIEVTFSKFKLADANKKDFVLEVKDSANDDASAISATELVFDANKKILTGKLNGLASNINYKISKFTLNNNEIKFDFKQEDQQELLNQYIQQAELNTIIDKANKKINIKLQNFSILNSFQDNQPVLTFDIEINKKDGITQVVHKSLTKNQLLNPNGLEIDLKDKMKNNIDYDVKLTNVKIANIALNNDNVNNI", "aa_hexdigest": "92de0f890fef4d75f3c5347f55f92f44", "start_type": "GTG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI000ABC946E", "ncbi_nrp_id": "WP_231845357.1", "uniref100_id": "UniRef100_UPI00016C0373", "db_xrefs": [ "SO:0001217", "UniParc:UPI000ABC946E", "RefSeq:WP_231845357.1", "UniRef:UniRef100_UPI00016C0373" ] }, "ips": { "uniref100_id": "UniRef100_UPI00016C0373", "uniref90_id": "UniRef90_A0AA86Z492" }, "psc": { "uniref90_id": "UniRef90_A0AA86Z492", "product": "DUF1410 domain-containing protein", "uniref50_id": "UniRef50_A0AA86Z492" }, "pscc": { "uniref50_id": "UniRef50_A0AA86Z492", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AA86Z492" ], "product": "DUF1410 domain-containing protein" }, "genes": [], "id": "NKIECMJPJD_1241", "locus": "GCA000828735_01238" }, { "type": "cds", "sequence": "contig_1", "start": 717336, "stop": 717659, "strand": "+", "frame": 3, "gene": null, "product": "DUF1410 domain-containing protein", "db_xrefs": [ "RefSeq:WP_231845358.1", "SO:0001217", "UniParc:UPI0004566093", "UniRef:UniRef100_D2EDJ6", "UniRef:UniRef50_A0AAX1QZA5", "UniRef:UniRef90_A0AAX1QZA5" ], "nt": "ATGAATTCATCTATTGTTTCTTTTGTAAAAAAATTAAACAAAAATGCTAAGTATCATATGGCTAATATCGCCTTTAACCAACAATTATTACCATATTTAAATAAACAATTCAATGTTAATGCTGATTTTCCAATTGATCTAGAGCAAAATCCAATCGACTTTAATGATAATAAACAAGCCAATGATGAAAAAATTAAAAAGACAAAAGAATCTGCTATAAAAGTAGCTACAGCAATTGCTAAACAAATTGTTGGAATAGATTATAAAGGAAGAGACATTATTAAAAATTTTATTGAATTAGATAATGTAGTAAATAAAAAATAA", "aa": "MNSSIVSFVKKLNKNAKYHMANIAFNQQLLPYLNKQFNVNADFPIDLEQNPIDFNDNKQANDEKIKKTKESAIKVATAIAKQIVGIDYKGRDIIKNFIELDNVVNKK", "aa_hexdigest": "0b1a047774e7e58afd6fa6b8eee69624", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0004566093", "ncbi_nrp_id": "WP_231845358.1", "uniref100_id": "UniRef100_D2EDJ6", "db_xrefs": [ "SO:0001217", "UniParc:UPI0004566093", "RefSeq:WP_231845358.1", "UniRef:UniRef100_D2EDJ6" ] }, "ips": { "uniref100_id": "UniRef100_D2EDJ6", "uniref90_id": "UniRef90_A0AAX1QZA5" }, "psc": { "uniref90_id": "UniRef90_A0AAX1QZA5", "product": "DUF1410 domain-containing protein", "uniref50_id": "UniRef50_A0AAX1QZA5" }, "pscc": { "uniref50_id": "UniRef50_A0AAX1QZA5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAX1QZA5" ], "product": "DUF1410 domain-containing protein" }, "genes": [], "id": "NKIECMJPJD_1242", "locus": "GCA000828735_01239" }, { "type": "tRNA", "sequence": "contig_1", "start": 717758, "stop": 717833, "strand": "-", "gene": "trnA", "product": "tRNA-Ala(tgc)", "amino_acid": "Ala", "anti_codon": "tgc", "score": 73.2, "nt": "GGGGACGTAGCTCAATTGATAGAGCACCTGATTTGCACTCAGGAGGTCGTGGGTTTGACCCCCATCGTCTCCACCA", "db_xrefs": [ "SO:0000254" ], "anti_codon_pos": [ 717798, 717800 ], "id": "NKIECMJPJD_22", "locus": "GCA000828735_01240" }, { "type": "tRNA", "sequence": "contig_1", "start": 717837, "stop": 717913, "strand": "-", "gene": "trnI", "product": "tRNA-Ile(gat)", "amino_acid": "Ile", "anti_codon": "gat", "score": 78.5, "nt": "GGAAGTATAGCTCAGTTGGTTAGAGCACACCCCTGATAAGGGTGAGGTCGGTGGTTCGAGCCCACTTATTTCCACCA", "db_xrefs": [ "SO:0000263" ], "anti_codon_pos": [ 717877, 717879 ], "id": "NKIECMJPJD_21", "locus": "GCA000828735_01241" }, { "type": "cds", "sequence": "contig_1", "start": 718153, "stop": 719385, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF07198.17" ], "nt": "TTGCTCCCCCTTGCTTCTTTTTTAATAATGTGCAAGAACCAAGAAGCACTAAAGACAAACCAAGAAGCACCAAAAACAAACCAAGAAGCACCAAAAACAAATAAAGAAAATGTCTTAGACGATTTTGATGCAAATATAATTAATATAGAAAATATTAGTAAACCTAAATTTATTAATCAAAATAAAACAATTATTAATTTAAAATTAAAAAAATTTAATTTTAAATCACCAAATACTACTATAACCTTAACTTATAAAGATAATAATGGTCACAAATTTACATCAGATCCTTTAAGTATTAATGATGAACAAAATTATGATTTTATTTTTTCTAATTTAACCCCTAATCGTAAATATCAAATTCAAAATTTAACTTTTAATAATCAAAAAAGAACAGACATTTATTTGAAAACCAACCTAAATAATGCTATTTTTTCAATAAAACCAATACCTATTAAAACCAATAATTTTAAAATAAAAGTTTTTAATCAAAATGCATTAATATCTTTTAGCATTCCTAAAAATTCTGATGTAAGAGTAAATGAAAAAATAGCATTAGAATTTGAAAATTTAAGTAGTAATTTAGTGCCTAATAACGAAATAATTAGTAGAATTGATAAAAATTTTAATGTAGAATTTAAATTAGATAATTTAAAATTAAATAATAAATATCGAATTGTAAATCTTAGATTTTTAGATACTAATCCACCTAATGTTAGTCCAAATATTTTTGAAAAGTTATCTAATTATGAAAGCAGTTTTATCATACCAGGTATTAAAACAAATATGCATAATCATAAAGACTTGAATAGTAATGACAAAAAATATATTGATAGTCCTTATAACACTAAAATTGAACTTAATGATAAAAATAATTTTAATGATAAAGTGTTGCCAGATGCATTTGAACAGCAAAACATTAATATACAAGAAGTTGAGAATCTTAATCAAGACATCTTAAATCAAAATGAGCTAGAATTACAAAAATATTTTAGTTTTATTAGTGATCAAGCAAACCTTAAAGACAAAGATTTTCAAAACTATTCTTTTAATGATATTAATAAAAATTCAAAGCAAGAAATTAAATTTAAAATCAAACATATTAACATCGATAGTAGTAATAAAAAAGCAATCATTGAATTAGATTCGTCTAGTTCTAATGATAATACTAAACTTTTAGAAGCAAATAATAAACAATTGTTAATTAAATCATATGATTATAATAATCCCTGA", "aa": "MLPLASFLIMCKNQEALKTNQEAPKTNQEAPKTNKENVLDDFDANIINIENISKPKFINQNKTIINLKLKKFNFKSPNTTITLTYKDNNGHKFTSDPLSINDEQNYDFIFSNLTPNRKYQIQNLTFNNQKRTDIYLKTNLNNAIFSIKPIPIKTNNFKIKVFNQNALISFSIPKNSDVRVNEKIALEFENLSSNLVPNNEIISRIDKNFNVEFKLDNLKLNNKYRIVNLRFLDTNPPNVSPNIFEKLSNYESSFIIPGIKTNMHNHKDLNSNDKKYIDSPYNTKIELNDKNNFNDKVLPDAFEQQNINIQEVENLNQDILNQNELELQKYFSFISDQANLKDKDFQNYSFNDINKNSKQEIKFKIKHINIDSSNKKAIIELDSSSSNDNTKLLEANNKQLLIKSYDYNNP", "aa_hexdigest": "1b57f96e4a2b34207e0749af699ad8f9", "start_type": "TTG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07198.17", "name": "DUF1410", "length": 56, "aa_cov": 0.13658536585365855, "hmm_cov": 0.9821428571428571, "evalue": 4.896349523932334e-14, "score": 48.07077407836914, "start": 75, "stop": 130 } ], "seq_stats": { "molecular_weight": 47727.1582, "isoelectric_point": 8.808524894714356 }, "id": "NKIECMJPJD_1243", "locus": "GCA000828735_01242" }, { "type": "cds", "sequence": "contig_1", "start": 719425, "stop": 719904, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATATTTGATTTACATGATTTTCCAAAAGATTTGAAAACATTTATTATTACTCATATTCGTTTTGATGATAATATAACTTCGCTTGGTAAAATTAAAGAAAATAGTTTTGAATACTATCAAAACGACAAGGAATATTTACTAAAATCACTTAAATACTACTTTGATATAAAAGAAAATCAACTATACGGTTCTGCTTGTTTTAATTTTAATAATGATGATTTTAAAATCCTAAAAAATAAAACTTTTGTTTTTAAATATGAAATCGACACTAAAAATAATATTTTAAATAAATATATACCATTAAATAAATATATAAATGTTGATTTTAAAAATCTAGCACAATTTAAAATTGTTAATGTTTTCGATGGATTAAATTATAAACTTGAAAGTATAAAAATAGTTAATAAAAACAGCTTGTTACCTTATAATGATCATGTTAATATTCAAAATGCTAATAATACAAATTTTAGTGTTTGA", "aa": "MIFDLHDFPKDLKTFIITHIRFDDNITSLGKIKENSFEYYQNDKEYLLKSLKYYFDIKENQLYGSACFNFNNDDFKILKNKTFVFKYEIDTKNNILNKYIPLNKYINVDFKNLAQFKIVNVFDGLNYKLESIKIVNKNSLLPYNDHVNIQNANNTNFSV", "aa_hexdigest": "5999f0a781d23e39b4d2f3f31464191e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19001.4157, "isoelectric_point": 8.439765739440919 }, "id": "NKIECMJPJD_1244", "locus": "GCA000828735_01243" }, { "type": "cds", "sequence": "contig_1", "start": 720043, "stop": 720615, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCGCAATTTAATATCATTAACTTTGCATGAAAGAGAATATGCAATTTATAAGCATAATGCTACTAATGGTTTTTATTTATATAACACAATAACAAGTAATAAAGATTTTAATTTAATTAAAAATAATAAAGAGTCATATCATCTAAATTCTTTAATTGCACATTTAGCAATTAAAGATGAAGGATTAAAAAAGGATGAATCTGCTGGTTTCTTCTTAGAAAAAGATTTGAATGATTTTGATAATTTAAATAATTTTAAAGATGAAGATATTGTCTTTAATGTAGATCTAGAATTAGATCCTAATCTAATTTATGAATCACAACTTGTAGATAAAAACATGCGAAGATCGCATGTGATAATTCCTATTTCATATAAAGTAATTAAAAAACAACACATTTTAGAAGATGTTGAATTTAGTTTAAATTATGCTTTAGGTTCTGAAGCTTATGAAAATCACATCTATCAACAAATTAAATCACAATTAAAATTTAATGTCTTTTTAAATGGTTCAAAGATAAAAGTTGAAGTTAAGCCACGCAATGATAATATTAAGCTTTATGATTACGTTTGA", "aa": "MRNLISLTLHEREYAIYKHNATNGFYLYNTITSNKDFNLIKNNKESYHLNSLIAHLAIKDEGLKKDESAGFFLEKDLNDFDNLNNFKDEDIVFNVDLELDPNLIYESQLVDKNMRRSHVIIPISYKVIKKQHILEDVEFSLNYALGSEAYENHIYQQIKSQLKFNVFLNGSKIKVEVKPRNDNIKLYDYV", "aa_hexdigest": "aa2a45eb10f05a877fc631d035092c1a", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 22384.070800000012, "isoelectric_point": 5.975903892517089 }, "id": "NKIECMJPJD_1245", "locus": "GCA000828735_01244" }, { "type": "cds", "sequence": "contig_1", "start": 720970, "stop": 721794, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAATATGAAAGTATTAATTTAAATAAAAAAACTATCTACGATGATGATGGAAATGAAAAATACACATATTCGAATTTTGCAGATTATACAGTTGTAATTCCTCAAACAATTAGTCAAAATGATTTAAATAAAGATAAATATCAACCATATTATAATTATAGTAGTGTTAATAACCAAGATACTCCAGGAAAAAATCAACTTCAATATTTTGCTTTTCCTTTTAAATTAAAATTTGAAAAAGTAATGGATTTTTGTTTACAAAAAAATAATATATATAAACACTACAATGCTTTAGGTAGATATGATGATAAAATAAGTGAATTAGATTGGTCAGTAGTTAGTATCGATCTAAAACCAATTTTTGAAGCTTTTAAAAATCAAGATTTAAATAAACCGTTTATTTACAATAATAAAACTTTGTCTCCAGAAGAAACTAGTGTTATAAAATATTTTTTAAGTTTAAAAAATATAAAACCATTAGAGGTTAGTCCACAAACAAGATATGTTAGAAGCAACCAAGATGTTGATTGGTATATTGGCACTTTCCCTCGTTATACAAATACTAACCAAAATTCAATGGGGGTTGGCGAACTACGTTATCGTGAATATAATATACAAAAAATCGATTCTGTAAATACTAATTTTGTCACAGGTGGTAAGGGCGTTTTATATAAATCAGATATACCATATACAACAATTAGTACTGACTATATAGATGCTGCGGGTGGTTCATCTGGAACTAGTTTATATGATGAACAAGGACGATTCGTTGGATCAATTGCAACTGGTAGAACTCCAAGTAAAAATGGTCATCCAACGTGA", "aa": "MEYESINLNKKTIYDDDGNEKYTYSNFADYTVVIPQTISQNDLNKDKYQPYYNYSSVNNQDTPGKNQLQYFAFPFKLKFEKVMDFCLQKNNIYKHYNALGRYDDKISELDWSVVSIDLKPIFEAFKNQDLNKPFIYNNKTLSPEETSVIKYFLSLKNIKPLEVSPQTRYVRSNQDVDWYIGTFPRYTNTNQNSMGVGELRYREYNIQKIDSVNTNFVTGGKGVLYKSDIPYTTISTDYIDAAGGSSGTSLYDEQGRFVGSIATGRTPSKNGHPT", "aa_hexdigest": "debffe87e0bf34e34466d2c1c9c8b918", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 31616.738200000043, "isoelectric_point": 6.18114948272705 }, "id": "NKIECMJPJD_1246", "locus": "GCA000828735_01245" }, { "type": "cds", "sequence": "contig_1", "start": 722045, "stop": 722317, "strand": "-", "frame": 2, "gene": "ylxM", "product": "putative DNA-binding protein", "db_xrefs": [ "COG:COG2739", "COG:K", "KEGG:K09787", "SO:0001217", "UniRef:UniRef50_Q9PR02", "UniRef:UniRef90_Q9PR02" ], "nt": "TTGATTGCTTTATATGATATTTATCAAGGATTATTAACAACAAAACAATGTGAATATTTTAATTTACATTATTTTAAAGATTTGTCGTTTTCTGAAATTGCTGAACTAAAAGAAATTTCAAAAAGCGCTATTAGTGATTGTTTAAACAAAGTGTGTGACCAATTATTAAAATATGAACAAGCACTTTTAATTTATGAAAAAAACAAAAAGCGTAATGATTTATATACATTAATCAACGATTCTGAATTAGTTAAAAAATTAAAAGATATATAA", "aa": "MIALYDIYQGLLTTKQCEYFNLHYFKDLSFSEIAELKEISKSAISDCLNKVCDQLLKYEQALLIYEKNKKRNDLYTLINDSELVKKLKDI", "aa_hexdigest": "3bda0ff222061b42ac69e5db5ac0977a", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_Q9PR02", "query_cov": 1.0, "subject_cov": 0.9090909090909091, "identity": 0.9890000000000001, "score": 176.0, "evalue": 5.07e-56, "valid": true, "gene": "YlxM", "product": "putative DNA-binding protein", "uniref50_id": "UniRef50_Q9PR02", "cog_id": "COG2739", "cog_category": "K", "kegg_orthology_id": "K09787" }, "pscc": { "uniref50_id": "UniRef50_Q9PR02", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR02" ], "product": "UPF0122 protein UU142" }, "genes": [ "ylxM" ], "id": "NKIECMJPJD_1247", "locus": "GCA000828735_01246" }, { "type": "cds", "sequence": "contig_1", "start": 722334, "stop": 722660, "strand": "-", "frame": 1, "gene": null, "product": "Cell division protein FTSY", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PYR8" ], "nt": "ATGAATGAATTAAAAAAAATTCATGATGTAATTCATAAATTTGATGAATACGCTCCGCACGAAACTTTATTAGTTTTAGATGCCACTCAAGGCCAATCAGGAATTAATCAAGCAAAAGCATTTAACGAAGTAACGAAAATTTCAGGGATTATTTTAACTAAAATGGATTCTACAAGTCGTGGGGGAATTGTATTAGCAATCAAAGATGCTTTTAATATACCTGTGAAATTGATTGGTTTAGGCGAAAAATTAGAGGATTTAAGTGTATTTGATTTAGAAATGTATGTTGATAGCATAGTTTTAGGAATGAAATTAGATGTTAAATAA", "aa": "MNELKKIHDVIHKFDEYAPHETLLVLDATQGQSGINQAKAFNEVTKISGIILTKMDSTSRGGIVLAIKDAFNIPVKLIGLGEKLEDLSVFDLEMYVDSIVLGMKLDVK", "aa_hexdigest": "85ac56b7a7f6a13f9182444ab3061336", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0PYR8", "query_cov": 0.9814814814814815, "subject_cov": 0.8346456692913385, "identity": 0.66, "score": 135.0, "evalue": 5.08e-39, "valid": false, "product": "Cell division protein FTSY", "uniref50_id": "UniRef50_A0A3B0PYR8" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0PYR8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PYR8" ], "product": "Cell division protein FTSY" }, "genes": [], "id": "NKIECMJPJD_1248", "locus": "GCA000828735_01247" }, { "type": "cds", "sequence": "contig_1", "start": 722814, "stop": 723086, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00448.28" ], "nt": "ATGATTTACCAAAAAGTTAATGATCCTGATTTAATAAAGTCTATTATTATTGATAAAATTTTTATTTATTATATTCAAGATACAGAAATTAATACAGCAATTAATTTAAAAGAAAATCAAACTAATGTAGTTTTGGTGGTTGGGGTTAATGGCGTTGGTAAAACAACATCAATCGCAAAAATCACTAAAAAATTCATTGATGAAAATAAAAAAGTTCTCTTAGTAGCTGGAGATACATTTAGAGCAGGTGCTGTTGAACAATTAAAAGTTTGA", "aa": "MIYQKVNDPDLIKSIIIDKIFIYYIQDTEINTAINLKENQTNVVLVVGVNGVGKTTSIAKITKKFIDENKKVLLVAGDTFRAGAVEQLKV", "aa_hexdigest": "49452ae69b358c9df4fa48f88203efc1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00448.28", "name": "SRP54", "length": 48, "aa_cov": 0.5333333333333333, "hmm_cov": 1.0, "evalue": 2.0027528688193877e-20, "score": 68.81938934326172, "start": 42, "stop": 89 } ], "seq_stats": { "molecular_weight": 10024.627199999995, "isoelectric_point": 8.891689109802247 }, "id": "NKIECMJPJD_1249", "locus": "GCA000828735_01248" }, { "type": "cds", "sequence": "contig_1", "start": 723171, "stop": 723389, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGTTTTTTTAAAAAAATATTCAATAAAATTCTAGGAAAAAAAGACACAGAACAAATATCACAAGAGATTTCACAAAAAAATGAAGAAAATCGTATTTTAAAATTAGAGGATAATTCGATTAATAAATTTAATGAAGGATTAAGAAAATCATCTTCTGCTTTAACTGATGCTATTAACGAATTAGCAGTTAAATATATAGATATTGATGAACGATGA", "aa": "MGFFKKIFNKILGKKDTEQISQEISQKNEENRILKLEDNSINKFNEGLRKSSSALTDAINELAVKYIDIDER", "aa_hexdigest": "45c1dad7f83261b1feabeedd33a3bb9d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8348.368799999997, "isoelectric_point": 6.257938957214355 }, "id": "NKIECMJPJD_1250", "locus": "GCA000828735_01249" }, { "type": "cds", "sequence": "contig_1", "start": 723392, "stop": 724072, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E5127C5", "UniRef:UniRef90_UPI001E5127C5", "PFAM:PF02463.25" ], "nt": "ATGAGTGAATCAGCAGCACGTTTAAGAATCGAAAGTTTAAAAATTGACCTTGAAAAATATAATGATATTAATTTAAATGCGTTAGAGGAATACGAAGAAAAACAAGCAGAATATGATTCTAATTTTTTAGAGTATGAAAATTGCAAGCAAGCGGTTGATCAAATTAGTAGTTCAATTCAAGAATTAGATAAAAAAGCTAAAGCTGATTTTATGCGTGTTATTCGTGAAACAAATAAATTAATGCCAGAGATTTTTGAATATTTATTTGGTGGAGGATCTTGTGCAATTAGTCTTAATGATCCTAATAATATTTTAGAATCAGGTGTTGAGATAATTGCTAATCCACCAGGTAAACGTGATGTTCCATTATCTGCACTTTCAGGTGGGGAAAAAACAATGGTAGTATTGGCAGTTTTATTTGCTTTATTAAAAATTGCAAAATTTCCATTAGTTATTTTAGATGAAGCTGAAGCAGCTTTAGATGCAAGTAATGTTGCAAAATTCGGTGCCATTATCGCTAAGTATAGTGATGAAACACAATTTTTAGTTATTACTCACCGCGAAGGAACAATGAAAAGTTGTGGTTATCTTTTAGGTACAACAATGATGAATAACGGAGTAACAACATTACTAGAAGTTGATTTAAATGATACAAAAATAAAATATGAAAGTGAATTATAA", "aa": "MSESAARLRIESLKIDLEKYNDINLNALEEYEEKQAEYDSNFLEYENCKQAVDQISSSIQELDKKAKADFMRVIRETNKLMPEIFEYLFGGGSCAISLNDPNNILESGVEIIANPPGKRDVPLSALSGGEKTMVVLAVLFALLKIAKFPLVILDEAEAALDASNVAKFGAIIAKYSDETQFLVITHREGTMKSCGYLLGTTMMNNGVTTLLEVDLNDTKIKYESEL", "aa_hexdigest": "0c49e3399df805220bcb72dd00c1d1dc", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 723392, "stop": 724372, "observations": { "mutation": [ 724357 ], "stop": [ 724357 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E5127C5", "query_cov": 1.0, "subject_cov": 0.5368171021377672, "identity": 1.0, "score": 424.0, "evalue": 1.23e-146, "gene_start": 1, "gene_end": 226, "reference_start": 196, "reference_end": 421, "reference_sequence": "MEVIVKKNIILNLEEKIKELDLKIKKLNEEQIELNNLHNQTKKQWQEYYNSEQIINGILNNLITTNNSLSDNLNNYKSQYEALSATKLSKEEIESKISDNWNEYNKKKNELINLKQKIQTLKANLNTANLNKNELEITYNKELEDFYNLDKNITSCESNIDNLNYSIQNVQTRLVDNYKLSVENAIETYQNQEIDMSESAARLRIESLKIDLEKYNDINLNALEEYEEKQAEYDSNFLEYENCKQAVDQISSSIQELDKKAKADFMRVIRETNKLMPEIFEYLFGGGSCAISLNDPNNILESGVEIIANPPGKRDVPLSALSGGEKTMVVLAVLFALLKIAKFPLVILDEAEAALDASNVAKFGAIIAKYSDETQFLVITHREGTMKSCGYLLGTTMMNNGVTTLLEVDLNDTKIKYESEL" }, "paralog": false, "identity": 0.9969325153374233, "score": 632.0, "evalue": 2.25e-231, "description": "Internal stop codon at 724357. Nonsense mutation around 724357.", "psc": { "uniref90_id": "UniRef90_UPI001E5127C5", "uniref50_id": "UniRef50_UPI001E5127C5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E5127C5", "UniRef:UniRef50_UPI001E5127C5" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E5127C5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E5127C5" ] } }, "hypothetical": true, "pfams": [ { "id": "PF02463.25", "name": "SMC_N", "length": 144, "aa_cov": 0.6371681415929203, "hmm_cov": 0.5902777777777778, "evalue": 1.5253824306623718e-25, "score": 85.44344329833984, "start": 65, "stop": 208 } ], "seq_stats": { "molecular_weight": 25076.376200000013, "isoelectric_point": 4.591703987121583 }, "id": "NKIECMJPJD_1251", "locus": "GCA000828735_01250" }, { "type": "cds", "sequence": "contig_1", "start": 724523, "stop": 725167, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF06470.18" ], "nt": "ATGTTAGAAGAGCAATTTAAACGCCAAACTGAGAATGAACGTGGTGTAAAAACAATTATTGATTCACAAAAAGCATTAGTTGGTGTTTATGGAACAGTTGCGCAGAATATTTCTACAAAAACAGAACACGAATTAGCAATAAGTACAGCTTTAGCTAAAGGAATCAATAATATTATTGTTAAAAACAATCAAGCAGCTGAAAATTGTATTAATTTTTTAAAGAAAAATAATGCAGGTGTTGCTACTTTTTTACCGATTGAAAATTTGCAGGCTAAAAGCATTAAACCAGAATTTTATGATGTATTAAAAGAATTAGAGGGGTTTGTAGGAGTTGCTAATGAACTAGTAAAAATTAATGTAAATTATAAAATTTCTATTGATTATCTATTAGGAAATACAATTATTGCTAATAATTTAAAAAACGCTATCATTCTAAGTGAATACACATATAAAAATTATAAAGTAGTCTCATTAGAAGGTGACATTGTTTTTGGTGGTGGTGCCATTTCTGGTGGAAGTAATAGTAAAAAAAATATTATTTTAAATCTAGAAGAAAAAATTAAAGAATTAGATTTAAAAATAAAAAAAATAAATGAAGAACAAATTGAATTAAATAATTTACATAACCAAACAAAAAAGCAATGA", "aa": "MLEEQFKRQTENERGVKTIIDSQKALVGVYGTVAQNISTKTEHELAISTALAKGINNIIVKNNQAAENCINFLKKNNAGVATFLPIENLQAKSIKPEFYDVLKELEGFVGVANELVKINVNYKISIDYLLGNTIIANNLKNAIILSEYTYKNYKVVSLEGDIVFGGGAISGGSNSKKNIILNLEEKIKELDLKIKKINEEQIELNNLHNQTKKQ", "aa_hexdigest": "ea36d13b25f56b5226cac383a6faf4cf", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF06470.18", "name": "SMC_hinge", "length": 118, "aa_cov": 0.5514018691588785, "hmm_cov": 0.9576271186440678, "evalue": 7.475969843623375e-28, "score": 92.87181854248047, "start": 28, "stop": 145 } ], "seq_stats": { "molecular_weight": 23809.04910000003, "isoelectric_point": 8.42738780975342 }, "id": "NKIECMJPJD_1252", "locus": "GCA000828735_01251" }, { "type": "cds", "sequence": "contig_1", "start": 725198, "stop": 725614, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATGATATTCAAGAAAATTTAAAAGAATATAAAAAAGAATATAATAAACAAAAACCATTAATTCAAAGTAAAAAAAATGAAATTGAAAGTTTACGAGTAATTTCAGATAGATATGCAAAAGAATACACTAATTTAGAATTTGAATATAAAAAATTAGAAGATACAATAAACGATTTGAATCTTAAATTATCTCTAACTAAAAATACACTTGAAACAACCGCTTTTAATGCAACAAATACACAAGAAAGGATTGATGCTTTTGCTACTTTAATTAAAACAACGGAAATAGAATTAAATAATAATAAAAAAAATATTAATGAAAAAAAAGATCAACTTAAAGATATTCAAAAACAAAAATTAGAACTTGAATCTCTTAAAACAAAACAAAAAAATAATTTGGATACCATCATTTGA", "aa": "MNDIQENLKEYKKEYNKQKPLIQSKKNEIESLRVISDRYAKEYTNLEFEYKKLEDTINDLNLKLSLTKNTLETTAFNATNTQERIDAFATLIKTTEIELNNNKKNINEKKDQLKDIQKQKLELESLKTKQKNNLDTII", "aa_hexdigest": "744cd3707d63ed262409c92eecb2d759", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16357.362999999985, "isoelectric_point": 8.580177879333494 }, "id": "NKIECMJPJD_1253", "locus": "GCA000828735_01252" }, { "type": "cds", "sequence": "contig_1", "start": 725624, "stop": 725857, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTGAAGAGGCCTCTGGAATCGGGCGCTATTCAAAGCGAAAGCAAGAAGCGTTAAATTCACTAAGTCGTGCAAACGAAAATTTAGCTCGTTTAAATGACATAGTTGCTAATTTAAAAAAAGAATTGGCGAAATTACAAAAGCAAGCAATGCGTTTTAATGAATATAAACAAATTAAGGACGAATTAACAAAATTAGATTTAGTGATTATAGTACGTGATATCATTCACTGA", "aa": "MFEEASGIGRYSKRKQEALNSLSRANENLARLNDIVANLKKELAKLQKQAMRFNEYKQIKDELTKLDLVIIVRDIIH", "aa_hexdigest": "32bd3a094b1b77a2c467e4aa0ecd0c8e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8972.318699999996, "isoelectric_point": 9.57021884918213 }, "id": "NKIECMJPJD_1254", "locus": "GCA000828735_01253" }, { "type": "cds", "sequence": "contig_1", "start": 725891, "stop": 726169, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCGTGCTCATAAAAATGATTTATTGTTTAGTGGTGGAAGATATGCACCGAAAGCCCATATTGCACGTGTTAATTTATATTTTAATAATGTTAATAATGTTTTATATACACAACATAAAGAAATTAAAATTTCACGAGTGTTGAATATTAAAACAAACGAAAATACTTATTATATTAATGATGAAGTTGCTCTTTTAAAAGATATAACAGATATGTTTTTAGATAGTGGATTATCAAAAGGTTCTTTAGGAATTATTAGTCAAGGTGCAGTGTCATGA", "aa": "MRAHKNDLLFSGGRYAPKAHIARVNLYFNNVNNVLYTQHKEIKISRVLNIKTNENTYYINDEVALLKDITDMFLDSGLSKGSLGIISQGAVS", "aa_hexdigest": "978daa1f22b254ead2b6a1f86e3838e1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10342.694799999996, "isoelectric_point": 9.300612068176267 }, "id": "NKIECMJPJD_1255", "locus": "GCA000828735_01254" }, { "type": "cds", "sequence": "contig_1", "start": 726197, "stop": 726337, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13175.12", "PFAM:PF13476.12", "PFAM:PF13555.12", "PFAM:PF02463.25" ], "nt": "ATGATTTTTTTAAAGAAAATAGAAGCACATGGGTTTAAATCATTTGGGGAGCCAGTTGTTATTGAATTTAAACACCCAATGACTGGAATTATTGGTGCAAATGGAACAGGAAAATCAAACATTATTGATGCTCTTAAATGA", "aa": "MIFLKKIEAHGFKSFGEPVVIEFKHPMTGIIGANGTGKSNIIDALK", "aa_hexdigest": "7bd4f6f5717002d627d8608e824c1503", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13175.12", "name": "AAA_15", "length": 42, "aa_cov": 0.9130434782608695, "hmm_cov": 0.9761904761904762, "evalue": 2.263459641752907e-09, "score": 32.88373565673828, "start": 4, "stop": 45 }, { "id": "PF13476.12", "name": "AAA_23", "length": 41, "aa_cov": 0.8913043478260869, "hmm_cov": 0.975609756097561, "evalue": 3.847798328151666e-13, "score": 46.05535888671875, "start": 6, "stop": 46 }, { "id": "PF13555.12", "name": "AAA_29", "length": 42, "aa_cov": 0.8913043478260869, "hmm_cov": 0.9761904761904762, "evalue": 9.121689634215566e-08, "score": 27.375383377075195, "start": 5, "stop": 45 }, { "id": "PF02463.25", "name": "SMC_N", "length": 44, "aa_cov": 0.9347826086956522, "hmm_cov": 1.0, "evalue": 6.919651027046663e-15, "score": 50.600250244140625, "start": 3, "stop": 45 } ], "seq_stats": { "molecular_weight": 4985.864200000001, "isoelectric_point": 9.304093360900879 }, "id": "NKIECMJPJD_1256", "locus": "GCA000828735_01255" }, { "type": "cds", "sequence": "contig_1", "start": 726399, "stop": 726590, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGATTTTGTTGTACAGTCTAAATCACAGAATTTTTTAAAAGCTTATCGTGTTCAAGATGAAATTCTAAAATCTGTTTTAAATTTAGGCGATAACTTAAATAATGAATTTTTAGAAGAAATCATGAGAAAATATGATACATACAAAATAATTGATAGAGATCATAAATATTTAGTGATCAATATTGAATAA", "aa": "MDFVVQSKSQNFLKAYRVQDEILKSVLNLGDNLNNEFLEEIMRKYDTYKIIDRDHKYLVINIE", "aa_hexdigest": "0074bf060c1f30ff2968a4a93c3605ca", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7611.6181, "isoelectric_point": 5.287413215637206 }, "id": "NKIECMJPJD_1257", "locus": "GCA000828735_01256" }, { "type": "cds", "sequence": "contig_1", "start": 726750, "stop": 727109, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATGAACAAATTTTTATTCAATTATTAGAAAACAATCTAACAAATTCAAAATCATATGTGACTCAACCAATAATATTAGAATTTAAAACGGATGTTTTTTTGATTATAAATCAAATTTTAAAACAATATAAAAAAGGTAAAATTATCAAAAATAATACAAATGAAATTATTTTAGAAATTGATAATAAATTAATTTCTATAACAAATTTAGATATCAATAAATTTCATTCGTATCAAATTGTTAATATTAATGAAAATAAAAATATTTATAACGAAAGGAATAGTTTAATTTTATTTGATTTAGTAAATTATATCATTGAAAAAAATCAAAATTCTATCAGACACATTAATTTTTGA", "aa": "MDEQIFIQLLENNLTNSKSYVTQPIILEFKTDVFLIINQILKQYKKGKIIKNNTNEIILEIDNKLISITNLDINKFHSYQIVNINENKNIYNERNSLILFDLVNYIIEKNQNSIRHINF", "aa_hexdigest": "812abe4bbe31cbb33079986e8e5836dc", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14171.160599999977, "isoelectric_point": 6.727485847473145 }, "id": "NKIECMJPJD_1258", "locus": "GCA000828735_01257" } ], "sequences": [ { "id": "contig_1", "description": "[organism=Ureaplasma parvum] [strain=SV3F4] [gcode=11] [completeness=complete] [topology=circular]", "nt": "TGTTTTGGCTAATTTTTTAAAAGAGAATGATTAAATGTGAATCTTAAAAGAATGATCAATAGTTATCTAATAATTTTTTTACCCTTTCTATCAGTATCGTTTGTGGTATTTGTTTTTTTGGGTATTTTATTAGAAGCTAGTTGATCTTTTTTTTATAGTTACTTGTTAGTTATTCCTTTTGGATTTGGAATTACACTTTTACGAATTTTCATTATTTCTAAATTATTAAATAAGCGTTTAAAAATGAATAATTTAGGTGTCAATCCCAAAAAATATCGTCGATTACCAGCAAATTTTTTTGCTTTTGGCACTATTTACATTTTATTTAATTTTCTAATATTAGTACCTTTGATCATTGGTAATCTTGTTAACCTTTATCAATCTATTTTTGAGACCATCTCATTATTTGTTTTAACATTTTTTATGTTTTTTATCCAAATGGGGATGCAAATTTATTTAGGAATTAAAAAAGATAAAGGAGAAAAAAAATAATTCATTATTCATTGGAGGTGAAACAGTGGAAAATTATAGCCCGCTTGATATAATGATTGCACTTCCACATATTGCTGCAATCATTATGGTAACTTTAATTATTGGAACAGTTTCGTTGATTTATTTTAGTATGGTTAAAAAGTTAACTGTACATGATGTGCCAAACCGTTTTGTAATTATTATTGGAATGATTGTTGATTATTTTAGAGGACTAGTTGTTGATACAATGGGCGCTAAACATGTTAAATTAGCACCTTATGTACTATTTACATTTTGTTATATTTTTACAGCTAACTTAGTTTCTTTATTTGGTTTCAAAGAAGCAACAACTGCATCTGCCGTTCCATTAGCAATGGCTCTTGGAACCGTGATCGGTGGTCAAATTGTTGCCTTAAAATACCAAAAAGCTTCATTCTTTTTAAAATTTGCTTTTAAAATTAAAGGTTTTCCAATTATGATAAACCCTTTGGAAATCGTTTCTAAATTAACCCCTATTATTTCATTAACATTCCGTTTATGAGGTAATATCTCTGCTGCTGCAATTTTATTAAACATAACATATTGAGCATTTGCAGGTTTTACAAACGTAGTTCCTTGAGTTGGTGTATCATTAATTGCTGCAGTAGTAATTTTACCAATCTTAATTGGTTATTTTACTTGTTTTGCAGGAACAATTCAAGCTTTTGTATTTACATTATTAACAAGTATTAACTGAGGTCTTGAAATCAAAGAAGGTGAAGAACACCACGCTCATCTTGCGCATAAAAAAGCCGAAAAACTTGCTGCTAAAAAATTAGCAGAACTTGATACTCAAAATCAAATTCAAAATAATGAGGCTCAAGTAGTTTTATAAAAAACTTATTAACGTTTAATTAAATTAAAAAATATTTAGGAGATATTTATGTCATCATTTATTGATATTACAAATGTTATTTCATCACATATTGAAGCTAATTTACCAGCAATTGCTTCTGAAAATGTAGGTTCACTTGCAAACGGTGCAGGAATTGCTTACTTAGGTAAATATATTGGTACAGGAATTACAATGTTAGCAGCTGGTGCAGTTGGTTTAATGCAAGGTTTTTCTACAGCTAATGCTGTACAAGCTGTAGCTCGTAACCCAGAAGCACAACCAAAGATTTTAAGTACAATGATTGTTGGTTTAGCATTGGCTGAAGCTGTTGCTATTTATGCACTTATTGTTTCTATCTTAATTATTTTCGTTGCTTAATTGTATTTTCTTTTTTTAAATAAATTTATTAAATAAAAATAATGAAAATTAATTAAGGGAGTAACGTTATGAAATTAAATAAAAAGCATTTAGTAGCTATATTATCCGTTCTTTCTTTATCAATTATAGTTGTACCACTGTTGACTTCATGTACTGGTGATATTCCAGAATTAAATCCAGCAGAGATTATTAATACACTATTCCCTAATGTATGAGTATTTATAGCTCAAGTTATTGCAATGTGTGTCGTTTTTTCATTAGTATTATGATTGGTATGAAAACCAACAAATAAAATGCTAGATAAAAGACGTGAATATATTGCTAAAGAAATTACAGATGCAGAAAATGCTAAACAAGAAGCTTTACAATATTTAGAAAACGCTAAATCTGAACATTTAGCAGCACAAGCACAAACGTTAGAAATTATTGCAAAAGCTAAAAGTGAATCATTAACTTTACGTGAATCATTAGAAAAAGAAGCACGTGAAGCTGCTGATAAAATTATTAGTTCTGCTAAAATTAGCATTGCTAATGAACGTAGAGAAAATCTTGAACGTTTACAATCTGAAGCTCGTGAAGCTGCTTATATTGCAGCTGAAGCATTGATGAAAAAAGAATTGTCTAGAGAAGATAATGATAAACTAGTTGATCAATTCATTAAAGAATTAGAAAATAATGAAAAATAATTTATGAAATCTTCATTAAAACCAGTTGAAAAATACGCTTATTCAATTTTTGAAATTGCTAAAGAAGAAAAAAAATTAGATTTATACAAACATAATTTGGAAACAATTAATTCTATCATTGAAGAGGTTCCAGCTTTTTTTGAAGCTGTTGGTGATCCTGCTCGTGATCGTAATGAAAGAAAACAAATTGTGATTAAAAATCTAGAAGGTGAGATTGATATTTATTTAATTAGTTTAATTGATCTACTAATTGATGTTAAATCGGTTAAACTACTTAAAAAAATAGTATTAAAAGCTTTGGATTTTGTTAATGAAGCTTTAAGTGTTAAAAAAGTTTTAATAACAACTGCTTATGAATTAACAAAAAATCAAATTGATCGCCTTCTGCAATCTTTAAAAAAGAAATATGTGTGTGAAAAAATAGAACCAATTGTTGTTGTTGATAAATCAATTATTGGAGGACTTTCAATTAATTTTGAATCGCAAGTTTTAGATAATAGTTTAAAAACTAAATTATTTAATATTGTAAAAAAGGTAAATTAGGAAGTAATTATGCAAAGTAATACTAAACTAGCAAAATATTCAAAAATTATCGCCAATCGTTTAAAAGCATATTCTAATCAAACTCGTTTTATTGAAATTAAAACTGCTTTTGAATTGAATGATGAACAAAAACAAAGAATTAAAAAAACTGTTATTAGTCGTTTTGGCGATGAAAGACCCATTAAATTTATCGTTGATCCATCATTAATTGGTGGTATATCATTAAAAATCAACTTGGAAATAATTGATTCTTCTCTAAAAACAAAACTAAATCAAATCATCAATACGAAAGAGAAAGAAGGTGCGTAAAAATGACAGATAATAAAAATCATTCATTAATTAGTGATATAAAATCACAAATTAAGAAATTTTCTGAAAAAGCTTTAACCTTAGAGGTCGGCAATGTAATTTCGTTAGGTGATGGAATTGTATTGGTTGATGGCCTTGATAATGTCATGTTAAATGAAATTGTACGTTTTGAAAATGGTGTTGAAGGAATGGCTTTAAACCTAGAAGAAGATGCTGTTGGTGTTGTTCTTTTAGGTGATTATTCAAATATTAAAGAAGGCGATCGTGTTTATCGTACAAAAAGAATTGTTGAAGTTCCTGTTGGGGATGTAATGTTAGGTCGTGTTGTTGACGCTTTAGGTAAGGCAGTTGATAATAAAGGTAATATTGTTGCAAATAAATTTAGTGTAATTGAAAAAATTGCACCTGGTGTTATGGACCGTAAATCTGTTCATCAACCACTTGAAACCGGAATTTTATCAATTGATGCAATGTTCCCAATTGGTAAGGGCCAAAGAGAATTAATTATTGGTGACCGACAGACTGGAAAAACAACAATTGCAATTGATGCTATTATTAATCAAAAAGGCCGTAATGTAAATTGTGTTTATGTAGCAATTGGTCAAAAAAACTCAACAATCGCAAATGTTGTTCGTGAACTTGAAGCTCATGGAGCAATGGAATATACAACTGTTGTTACAGCTAATGCTTCAGAATTACCTGCTTTGCAATATATTGCTCCCTTTACAGGAGTAACAATTGCTGAAGAGTGAATGCATCAAGGAAAAGATGTTTTAATTGTTTATGATGATCTAAGCAAACATGCTATTGCTTATCGTACTTTATCCTTATTATTACGTCGTCCGCCTGGTCGTGAAGCATATCCTGGAGATGTTTTTTACTTACACAGTCGTTTATTAGAACGTGCATGCAAACTAAAAGATGAATTAGGGGCAGGATCTATTACTGCTTTACCAATTATTGAAACACAAGCTGGTGATATTTCAGCTTATATTCCAACAAATGTAATTTCAATTACTGATGGGCAAATTTTTATGATGACATCATTATTTAATGCTGGTCAACGTCCTGCAATTGATGCAGGGCAATCAGTTAGTCGGGTAGGTTCTGCTGCACAAATTAAATCAGTTAAACAAACTGGAGCATCATTAAAATTAGAATTAGCTAACTATCGCGAACTTGAAGCATTTAGTCAATTCGGTAGTGATTTGGATGATGAAACTAAACGTATTCTAAAACTTGGTAAAGCAGTTATGGCTGTTATTAAGCAAGCTCCTAATAAACCTTATAATCAAACTGATGAAGCGATCATTTTGTTTACCGTTAAAGAAAAACTTATTCCTCAAGTTCCAGTAGAACGTATTCAAGATTTTAAAGAGTACTTATTAAATTACTTTAAAGGAACAAAACTACGTGCTGATCTTGAAGATAAGAAAGCTTTTGATAAAGAAAATACCCCAGCATTTAGATGTGCTATACAAAAAGCAATTAACAGTTTCTTAAATAATTCACAAGATTTTAAACCTTGTGAAGAAGCTGAACAAACAGCCTTTGATAAATTCTTTAATGAGAATGAATCAATTGTTGTTGATGGTGAAAATGATTTTAATTTTATTAATGAAGAAGTTAGTTTAAAACCTACAACAGAAACATCTGAAGCTGTTCAAATAGAAGAAAAAGTTCAAGATTTCGTTGAACCACAAGAAATTCTTGAAACTAATAAAATGGAAGAAAATCATATTTTTGAAGAAGTTGAGCCAGAAAAAATTATCTGTGAACATCATGAATTTGAAATAGCTGAAAACCAAGAAAAAATCGAAGGTCAACAAGTTTTAGAAGATACAAACCATGAATATTCAATTTATGAAACCGTTGAACAAAGCGGTGAGGTAGATAATGATGAATCTAAAGATGATGATTTAGAAGTCTTAGTACCAGTTGCTGAAATTGAGCATGATGAAGCAATTCTAGATGAACGCGAGAATCGTAATTGAGTCTTCTCAGATTCAGCTGTTTCTGAAGTTGAAAAACAAACAATTATGATTTCAATTTCATCAAATGAAGCAGAACAATTGTTTGATAATGCTAAAAGTGTTGTTTTCTTTAAAGTAACACCTAAATATCCAGTTGAAAAAGTATTAGTTTATGTCACAAGTCCAGTACAAAAAGTTGTTGGTGAATTTGATTTATTAAAGATTGATGTTAATTCAGTAAACACTTCTTGAAATAAATATCGTTCAAGTTCAGTAATTTCATCACGTAAAGAATATTTAGAATATTTTAACTCACACAAAGAAGCACATGCTTTATTAGCTTCTAAAGTTTATAAATATCGTAAGCCAAAAGATTTAGCAAGTTTTAATATGAATAAAGGACCAAGTGGTTTCACTTATCTTAAATAATGAATCAAATTAAAATGAATAAAAGCTTTGCTGAATTCAGCAAACTTCCAAAAATTAAACAAGTCTTTACGCCTTGATTTATAGCAGCATTAGTTATATTTATAGTAGGATTAACAATAGCGTTTACAATGGTTGGTTTATTAAATGAAGGTTTTGAAAATGCAAGAGCATTAGTTAATGATCAAGGTCAAATTATTGGTAAAGTAGCTAATGTTAGTGGTGATAATCATTCTTTAATGTCATGATTAGGTTATGATCAACACGCTAATTATCAAGAATTGTATAAAACAATTCACGATCAATTGATTAATGGAAAAACAATGGAGTCATTAATTAATAATACTAATAAAAATAACGATCCAAAATTATATGAAGCTCTTCATTATCATGCGCATAGTAAATTTATTAATCATAATGGAACATGAGGATTTGTAACAAAAGTATTAAGTGATTGAGAAAATAGTTGATTTTACAAATATAATCAATTATTCTCACAATTAGCTAACTCACAAAATCCTGTATTAGCAGCTCAAGGAATAAAATCTCTTGCAAAATTATCAACCATTATACAATATCAAAATCCAAATTATATTGCATATAATTGAGTATTTATTGTATTTATGATGCCACAAATGATTACTTTTATAATTATTGTTGTTAAACTAGCGACTGTATTTTCACCTAAAAAAAGTGCTGAAGAAAAACAAGCTTATAAACTTGCAAAACTTGAAGTTAAAAATCAAAAAAAGGCTAAAAAATTAAATAATTCTTTACAACAAATAAATTTAAATTTAGAGGCAAACTAGTATGTCTTTAGATGCAATGAAACGAAAAATTAACTCAGTTCAAACAACTGCTAAGATTACGAATGCAATGAAACTAGTGGCAACTGCAAAATTAAAACGTCAGCGTGATCGTTTAGCTGCAATTAAAGAATATTGTCATGATTACTATGATGTTATTGGGTTATTATTGTCTGTTGTTGATGATGTTGAATTTTTAAAAATTCCAAATGCGAAAGATCGAACTTTATATATCACTATTAATTCAACAATGGGTTTAGCGGGTTCTTATAACTATAATGTTAATAAATTAATTTCTAAAATTGTAAATGAAACTGATATAACATTTACAATTGGAAAAAAAGGACATGACTTTATGCGTTTATCAGGTCGTGTTGATCAAGTTAATACTTATTTGAATTTAAATGATAATGATTTAACATTTGACATGAGTTTACAAATTGCGCGCGAGGCTCTTGAATTATATTCACAAGGTGAAGTTAATAAAATTTGTATTATATATACTAAATTTATTAACGCAATTACTTTTGAAGTAAGTGTTATAGATGTTCTTCCATTCGATAAGACAGCTTTAATTAAAGATCATCTTGCTGAAACTATTGAATTAGCAAAAGATAATATTATTTTTAAACCTAACAAGTTTGAGCTTGTTAAAAAAATTCTACCTACTTATATTGCAACAGTTCTATATGGTTCATTAATTGAATCAAAAATCTCTGAAAATGCATCACGACGCAATGCAATGGATGCAGCTACAAAAAATGCCAAAGCATTGGCTGAAAATTATAAATTAATTTACAATACATTACGCCAAGGTAAAATTACTCGTGAAATTACTGAAATTGTTGCTGGTAGTGATGATTAGAAACTATAAACAATAAGGAGCAAAATATGACAGAAGTTAAAAAAGGTAAAATTAACCAAATTTTGGGCCCTGTTGTTGACGTGCGTTTTCCCTCTGAATGATTGCCTGAAATTAACACTGCTTTAGAATTAAACAATCATGGTTCTAAATTAGTACTTGAGGTTTCTCAATTAGTTGGTGATAATATTGCAAGATGTATTGCAATGGATACAACTGATGGACTAGTACGTGGACAAGAGGTAATTAATACTCAAAAACCAATCACAATGCCTGTTGGTAAACAAGTACTTGGACGTATGTTCAATGTAACTGGTGATCCAATTGATGAACAACCTGCACCAACAGGTAAACGTATGCCAATTCATCGTCCTGCACCTTCATTCGCCGAACAAGCAGAATCAATTGAAATCCTAGAAACGGGAATTAAAGTTGTCGACTTACTTGTACCTTTTGCAAAGGGTGGTAAGATCGGTTTATTTGGTGGTGCTGGAGTTGGTAAAACAGTTTTAATGCAAGAATTAATTCATAACATCGCTAAAAATCATGGTGGTTTATCAGTTTTTGCTGGAGTTGGAGAAAGAACACGTGAAGGAAATGATTTATACTATGAAATGGCTGAGTCAGATGTTTTAGATAAAACAGCTCTTGTCTTTGGACAAATGAATGAACCACCAGGAGCACGTATGCGCGTCGCATTAAGCGGTTTAACAATGGCTGAAGAATTCCGTGATGCTTTTGGTCAAGATGTTTTATTATTTATCGATAACATTTTTAGATTCACACAAGCTGGTTCAGAAGTTTCTGCTTTACTTGGACGAATGCCATCAGCAGTTGGTTATCAACCAACACTTGCTTTTGAAATGGGTCAATTACAAGAACGAATTACATCAACTAAAAAAGGATCAATTACATCGGTTCAAGCTGTTTATGTTCCAGCTGATGACTTAACTGACCCCGCACCAGCAACAACTTTTTCACACTTAGATGCTAAAGTTGTTTTAGATAGAGCTATTGCCTCATTGGGATTATATCCAGCAATTTCACCACTACAATCAACAAGTCGTTTATTAGATCCATTAGTTGTTGGTGTAAAACACTACAGTGTTGCGCGTCGTGTAATTGAAATTTTACAAAGATTTATGGAATTACAAGATATTATTGCAATTCTTGGAATGGATGAATTAAGCGAAGAAGATCGTCAATTAGTAATGCGCGCACGTAAAGTACGTAACTACTTATCACAGCCAAGCCACGTTGCTGAAAAATTCTCTGGTCAACCTGGATTATCAGTTAAACTTGAAGATACTATTGAAGGATTTAGAAAAATTCTTGATGGTGAATGTGATGATATTCATGAACAACATTTCTTATATGTTGGTAAAATTGATGACGTTTTTGAAAAAGCAGCAAAAAATAAATAATGGCTAATTTAACTAAATTAAAAATTGTTACACCTTATGCACAAATTTTAGAAAAAGATGTGTATAGTGTTGAATTAAAAACATCAGAAGGACGAATTACCGTTTTACCTGACCATAACCCTTTGATGTCAACAATTGAAAACCATGTTGCTTATATTCGTGAATTACCAAATACTCCACGTAAACCACTATTATTATTAGATGGTATAGTTTATATTGAACAACATCAAGTTCGTGTTTTTAGTGACTATTTTAAATTTTTAGATGAAATTCAAATTGATGAAATTAATTCTTCGTTAAATCAATTAAAACATGATTTAAATAATGAAGAAGATGATAAGAAAAAACTTCAATTAAAATCTAAAATTAAATTAAACGAATCAATTTTAATCGCTTATAAAGATCGATAAAATAATAAAGACAAGAGGAATTTTCTTCTTTGTTTTTTTTATTTTTTTTAAAAAATAAATAGCCCTTAAAATAAATTAATTATGTTTTAAATTTATCTAGTTTAATCTATGTTAAAATAATTAAAAATAAATTAAGGAAAATTATGAAGATTATTGGGTCAGCTTTTTTAGGAATAGTTTTTTGCATTCTTTTGGCTTTTGCAATTATTTTTGGAATCGAAATTGATTATTATCATCAAGGTGATTATTTGAAATATCTTAATTTTTTAGATAAATTACACCAATATAATAAAATTGATAATTCATTTGAATACTCAAATCATTATGAATCAGCATTAATTGGTGTAATTGTTTTAACAATTATTTGCTTTTTAATATTCATAACACCAATTATAATAATAGTAATAACAAAGATTAAAGAAAAGAAAGTAATTAACAAAAAGATTTAAAGATAAAACGGTGTTGATTTTAACAAAACGCCGTTTTATCTTTATTTATCAATTCTTTTTAAAAAATTTTATTCTTTTTAATATATAATTAAAATCCTTTAAAAAAAATAAAATTATTTATCAATTCTTTTTAAAAAATTTTATTCTTTTTAATATATAATTAAAATCCTTTAAAAAAAATAAAATTATTGACATTATATGCAAAAAAAAATATTAAAAAACTTATCAATAGGTGTTGTATCAACAATAATTCTTGGTTCAATAACTATATCATTATCTAATTGTTCTTATAACAAAAAAACATTTTCGTCTTCTACAATTACGAATCAAGATACTAAAAATTTAGACATAATAGCAAAAAACATTAAATTTAAATATGATATTTTTGAAAATGAAGATGAGCAAATAATTAAAATTAATAAAATTAAAGTTGTGGGTGACTTGGGGCAAGCTAAAATAATAATTAAAAAAGTTATTATTGAACAAAAGTCAAATAATTTTTTTGTTCAATACCAACTCAGTTTGCATAACCAAACTAAAGAATATACACAAAACTTAGAAATCAATAAGAAGCAAAGTGTTATTGAAACAAAATATATTAATGATGAATTAATAAATAATGATTATCGTCCTTTTGCATTAAAACAATTATTATCTTCACCGCAAAACATAAAAAAAACTAGTGATCTTTCTTTAATAACAATGCAGAAAAATAACCAAGAATTTAATAATTATTTTAATTTTATTCAAAATGAAGATAATTTTAAACAACTACCCTTTAATCAAGCAACGCTCTTAACAAAACAAAACCAACTAACAGATCATCACACTAACTTTAAATTTGAAAAAATTGAAATAGATAAGCAAAACCAAATTGCAACAGTTAGTTTTAGTCATGATTCTGATTCACAAACAATACAAAAAATATTAAATGCGACAAATAAACGTCTTTTGATTAAAAATTTTGATTGAACCAATCCTTTTAGCAAAATTATTTCATATGAAAAAATTAATAATAATCAATTACGCTTTTCACTAAAAGATTTTCCTAAATCATTAACAAAATTTATCATTACTCATATTCAGTATGATGATGTTGTAGAATCACTAGGACGTGATTTAAAAAATTTATTTTTATATAATCAAATTGAAGAAGAGTTAACACTCAAAAAACTTAATTTCTATTTGCAAGAAAATGAAGTATATGGATCAGCATTTTTTGATTTTAATGAACAAACTTTAAAATTATATAAGAACAAGATTTTTTTATTTGAGTTTACTCTAGACGAAAATTATAGCGAAGCTAAAAAAAATCAAAGTTTAATTGCAAAATATACTCCACCAATTCAGTATGTTCGTGTTCCTTTTAAATCGCTATGAAAATTCAAAATCCCTCATTTATATAATGGATATTTATACAAATTAAACAGAATTAGTGTTCGTGATAATAATAGTTTAACGCCTTTTGCTTTTGTTAAAGTAAAAGAATTAAATACAAGTACTTTTTTTGTAAAAAATATAATATATAATGATCGTAAAATTATTGATTTGTATTACAATAATAATCAGTTTTTTTCAACAAAATATCAAACTTTAAAGAATAAAAATTTAACTTTAAATGACTTATATAAACTTTGAGCATCTAATCAAAATAATTTATATTTAGATTATTCATTGCGAAATTTTATTTCATTAGTTCAATATGAAAATACCTACTTATTTTATCAAAATAAAACAAACGAAGGTTTTTATGATTTCATAACTAAAAAAGCTGATTTTATACTTGTAAATAATAATGAAATCAAAAATTTACATAAAACTACATTAAAAGATAATCCAGATTTGATCTTTAAAAAAGATAAAGATAATTATTTTCAATTAACAAAAAAAATAAATAAGATTAAAAATTTAAATCTTGTAAATGAAAAAAATTTAGTTTTTAATATTACTTTTGGTTTAGATCCAAATCTCATCAAAGAAACTAACCTGTGAAAAAACCTTATGTTAAGGTCTCAAATTTCATTAGCAGTCCCTTATAAAACTTTAAAAAATAAAAAAGTTATTGAAGATGCTGAATTTGTTTTAAATTATGGTATTGGAAATCAAGAGTACGAATATGAACTCTACAAAAAAATTAAGCAAGATTTTCGTTTTCAAATATCTTATATAAATGATGAATTAAAAATTAAAATTATTTCTAAAAATAACATTAGTGTTTTTGATAATATTTGAGAACATAATTCTTCAATAGATTCATCTTATTTTTTAACAAAGGCTAATTTTAATATTTATTATTTAAATAATACTACTATAGATATAATGGAAGATGATAAATCTAATAATCAATATACAACAAGAATTTATAAAAACAATGAACAGAATTTTTTACCAATTAAAAATGCTCGTGATCGTGTAATTTCATTAAGTTTAATTCAAGATGGTACATGAAATGTTCTAGGGAAGGTTAAACCTAATGATGATGATGATTATCGCTATTATCTAATTAGTTGTGCTCATGTTTTAAATAGTACGCAAGCTTTTAAATCAATTAACAAAACAGGTAATAAAGATCTCTTTGAATCAAGTAATAGTTCTAATGGAGATTTTGATCCACTAGTTCCCCAAATTATTCATGAAAATGAAACATTAAATGATAAAAAAAATCCTTATTACAATGCACTTGAAAAAAATGGCAAACTTGAAGCTAATCAAAATCAATTTCAATTTTTAAATTTTCCTGAAAAAATTAGTATCGAATCGATCATTAATTTTGATCATCAAGTAAAAAATAAATTATCCCCTCATGTTAACGCTTTAGGTTTTTTTAATCCTAATGCAAGTTTAGATTTTTCATTAATTACCATTAATTTAAAACCTTTTTTTCAAAAATATGGTCAATTAGAAAATTCACCTGCTTTTAATGATTTAACAAAAGAACAACAGAGTGTTATTAGACATATAATTAATTTTAAGGATCTAAAACCTTTGAAATTAAGTAATCAAACTAAGTATTTCGATGATAATCAAAATGTAAAATGATACTTGTCAACTTTCCCTAGAACAGAGACAATTAATGAATTTAATGCTGGCAAACTTCCACGCTATCGTGAATATGAAATTAATAAATATGATAAAATCCAAGAAAATATTATTACTACTAATAATTACTTATCAACAGTAGGGACAACTAAAATTAGTAATCAATATTTTGATGTTGGTTCTGGTTCATCAGGATCAGTAGTTTATGATGAAAATGGTGATTTAGTTGGAATGATTTCTTTTGGCGCCCATGAAAATGCAAAATTTACAGCCGGAGGTATGATTGTTTTTGATTCTCTAATTTCTGGATTTTTTGGAGATAAAAACAATTTCGCTAATCGTACATCTTTAATTCAACAAATCAAACAATTAGCCTATTTATATCCAGAAAAATATGAAGATATTTATAAATAGAAAGGAATCTTATGAAAAAAAATAAATATAAACTTCTTATTAAATCACTAGTTATTACATCTTTTTTAGTTACTACTTCAAGTGTTATTGTAGCTTGTACTAATAAAGAAAAAGAGCAACATTTAACTGAATTAAAAAAATTATCTCAACATGCAAAACAAATATATAATGAAATTAAAACTAATGAATTATTTAAAAAGGAGGCTTCTGTTTTAAATGATCAAATTAATAAAGTTTTAAAAATTAATGAACAAAATAATTTAGATGTCATTACTAAAAATTATAATGATTTAAAAAAATTATTAGATGAAATTCAAGAGAAAATTTCTAAAGAAAAAGAAAAAGAAAAGCAACAAAACGCTTATCTTAATAAAGAAATTAACATTAAATTAGAAAATTTAAATCAAATAGTTAATCATAATTTATTAAAAGGAAATAAAGAATCATTTTTTAATCAAATTATTAATTTCACTCCAATTACAACAGAAATTCAAACTATTATCAATCAAAATCAAAATCTTTTAGATTTCAAAAATGAAGAACTTGAATTAAAATTAAAAAATTTAGACCTTTTCATTAAAGAATTAGATCATAAATTTAAAAATATAATAGATCAACTATTTAATATTGAAGATTCATACATTAATAAGTTTCTAAAATTTATTAATAACCAGGACTTTATTAAGGATCAAAAATATCAAGAAAGAGAAAAGTTTAATCCGCAAGAATATTTAGAATTAACAAAATCTATTCAAAGTAGTTTTAATAAATTAAAAAACGTTACTATCAATTCAAACCTAGACCAATACCTAAATTCACTTAATTTAGTGTTATTATCTTTAACTAATGAAACTAAAGCCTTTTTTACTAAATTTGAAGATCAAATTAAAAAATATTTTAAGTGAATTGTATATGGTAGAGATGATACACTAACAAAAGCCTTTAATCGAATTGTTGAAGAACAAAAAAGAAATGAATTTATAGAGCAAACAGAAAAAGCTTTATTAACTTTTCCCATGGAAATTAATCAAAAATTTAAAATGTTCGTTGATTTTTTTACATTAAAAAATGATAAAAACGAAACTGAATATCAAAAAATCTGAAAGTCAAAACACAACGAAGTTAATAAAGTCTGATTTGGTATTGCAGAGTGTTTTGCAACTGGAAAATGTACAGACAAAAATAAACCATCATAAAAAAATAACTAGGGTTTTATTTTAGTGCGATAATGCGCTAAGATAATTCCTGTTGCTACACCAACATTTAAAGATTGTACATCACCAAACATATCAATTCGTATGCTTTGATCTGCATTTTTTAAAATTAATGGACTCACACCTTTATTTTCATTGCCTACAATAAAAACAGTTTTATTAGCATAATCAACTTTTTCATATGGTTGTGATTTTAATAAACTTGTCGCATAAACTCAAAAACCTTCATCTTGAAGGTTTTTTATTGTATTTGATAAATTATTGACTTCGATCATATAAATATAATTAATTGCACCCATACTTGTTTTAATTACTAAATCATTAATGCTTGCTTGGTGATGCTTTTTATAAATAATAGCATCCACACCAAAACCATTAGCAGTTCTAATAATTGCTCCAAAATTGCGAGGATCTTCAATTTCATCAAGTATTAAAACTAAAGCATTTTTTTTATCTTTTATTAATTTTAAAAAAGATTCTAAACTCGTTTTTTGAAAGTTTTTTTTGACTAGTTTAAATGCAATATTTTGATGGTTTAAAGAATGATCAAATTGTTTAAATCATGCTTCATTAACAATTTCATAAGTAATTTTATTTTTATTAATTAAATTAATTAACTCATAATTTTTTGATAATAAATACACTTTTGAAATTGATTGTTTGTTACCAATTGCATCTAATAATGCTTTTTTGCCAAAATTATAATTCATTTTATAAAATTCCTATATTAATTAATTGTTTTCTTAATTCATCAGCTTTAACATAATCTTTTTTATCAACGTAATTTTTTCAAGTATTTAATAATGCTAAATTCTCATCATTATGCAAATCAACAAAATTAATACCCAATATATCTAACATATATAATAATTGAGTTAAATTCAGTTTTAATTCATTCATATCCTTATTTTCTTTAATTAAAATATTTATTTTTTTAACACTAATATGAATTAGATCAACAATACCTACAAAATCTAAATCATCATTAATGCGTTCATCAAAAAGCTCAAAATGATCAATTTGTGTTAATGATTTTAGTTGATTATTGAAGTATAAATAGTTTTTTGCGTTATAAATAGCATTTTTAATTTTAAGAATTTCTTTTTCGTTCTGCTTCATGATTTCATGCGAAAATTTAATTGGTTGCTTATAATCTGCTTGATAAAAAAACCAACGCACAACTTGATAATCGTATTCAGCTAATAAATCTTTTACTAAAATAAAATTATTTAAAGATTTTGACATTTTCTCATTATTAATATTAACTAATCCAAAATGCATTCATACTTTTGCTAAATTTTTATTATATAAAACTTGATTTTGAGCATTTTCATTTTCATGGTGGGGAAATTTTAAATCAATTCCACCACCATGAATACTAACTTGTTCACCAATATAAGTGTTGATCAAACATGAGCATTCAGAATGCCAACCTGGGCGACCGATTCCTCAAGGTGTATTTCACATAATACCTTTATTTGTTTTCTTTCATAAAATAAAATCATTAGGATTGTGTTTATTAGCTGCGCTTTCAATACGCATACCAACAATATCATTTTCTAAATCACGTTTCGATAATTGTCCGTAATTTAAAGCTTTTTTTGTATCAAAATAAACATCATCACCAATAAAATAACCTGCTTGTTTATCAATCAATTTTTGGATATAATCAATAATTTTATCCATATGTGTTGATACTTTAGGATTAATCATTTCTAGTGTATTTAAAGCTTTTTTAATTTTTAAATATTCATTAAAATAATATTCACTAACCTCTAATTCTTTTAGGTTATTAGCTAGAGCATAATTAATGATTTTGTCATCAATATCAGTAATATTTAAAACATATTTAGTTTTGATATTATGTTTTTTTAAAAAACGATATAACACGTCCATAGTAATTAAAGGCCGAGCATTGCCAATATGGATATGATTATAAACGGTTGGTCCACAATTATAAATTGAAATTAATTCATCATTAATTTCTACTAATTGATTAGAATAAGAATCATATAATTTCATAAATTATTCCTTGTTAATATGTAATAATCGTTTTTTTATCTGCAAAAATTTTTAATATAGTTAATGATATAAAACTAATAATGATGTTTTTAATTAAATTAAAACTTGTATATAAAGCAATAATTCCTAATCAATATGATTTAATATTTAACAAAAATAAAGTCGGGTGCTTTTCATAAATTTTTTGTACTTCTAAAAAATTCAGTGTTGGGGTAATTTTAAAAGTGTATCAATAAAGTGGAGTGTAAAGAATACCGTTCATAATCACATTAAAAACAGTTGTTAAGAATGAACTTAATACTAAAACTCATAAATTAAGATAAAATTTTGGTCTTTTTGGAATAAATAACTTTTTAAAAAGAATAACCATAGTAACAAAAAATAAGTTATTTAAACTTAAAACCATTGTTCCGATCCATGAAGGACTTCCGTGCAATAAATTAGCTAAACTTAATAAAATTGTAACTAAATATACAAAGCGATATTTAACTTTATAAATTAAATAAACAAGTGTAAACAAAGAAAAATCAAAAGCTAAGAAATTAAAACGAATAAAGTGACTTAAATATGCAACCAAAATTCCTAAACCTAACATTACTGCACTTAGTGTGTAGTCTTTAATAATTTTAGATGTTGAATAATATATACTATGTTTTTTTTTATAATTTTTTTTAAAAATTGTTTTTTCTTTTGTTATATTTATCTTCAATAATTTTTACACCAATACCTTTACTATCTTTAATTAAAGCGTTAATTTAACTTTAATATAATGCATTACAATTATTTTAATAGTTTTATCTTTCTTAATTATAAAATATATACATTTTAAATTTTATTTTAAGAGCATATTTATTACAAAATTATTATTTTCTATATCATAGAAAACATTACAATAATTTTAATTTAGCAGCAAAAACAACTTAAAATTATTAATATTCAAGTAATTTAAAAATTATTATCCTTTTTTCAAAATTCATTTTCAATAATTTTCACACCTAATAATTTAGCTTTTTCTAATTTCGTACCCGCTTCAGTGCCAACTAAAACATAATCTGTTTTTTTAGAAACACTGTTTTTAACTTTTGCATGATAATATTTCTCTAAAATAGTTTTAATTTCATCACGGCTAATACTAAATGAACCAGTAATAACAAAAGTTTTATTTTTATATTCATCAATTATATTGATGTTATCATCATAAATAAAATCATCAATATATTCCATATTTACACCAACTTGTTGTAATTGATTAACTAAAGAAACATTATGCTCATCATTAAACCAATTAATTAATGATAAGGCTGCTTTTTCCCCACATGCATCAACATTAGTTAATTCATCAAAATTAGCGTGCATTAAAGCAGTCATTGTTTTAAATTGTTTAGCTATTTTTTTAGCTAAATTTGGTCCAACATGACGAATTCCAAAAGCTGTTAATAAAAATTCTAAAGAACATTTTTTTGAATTATTAATACTATTAATTAAATTACTAAAACTTTTTTCTTTAATTTTAAAATCACTAGTTAAAATAAATTCTTTGTGTTTTTGCAAATAGTATAAATCTGCAATGTTTTTAATTAAATTAGCATCATAAAATTTATTAATAATGGCATCACTCATTCCCTCAATATTCATGCAATTACGTGAAACAAAATAAACGATTTTTTCAATATTGATTTTTTTACATCCATAAATATTTAAGCAGCGTTGGTCAACTTCATCATTTTCACGTACTAAAACTGATTGGCATGAAGGGCAATTTATAATTGGTTCATAAGGTACACTATTTGCTAAACGTTTTGATAAATCAACACCATCAACATAAGGAATTACATCCCCTGCTTTATAAATTTTAATTTCATCATTAATTCGAATGTCTTTTTCTTTAATAAAATCAAAGTTATGTAAAGTAGCGTATTCAACCATACTACCATCTAATAAAATTGGTTTAATTTTAGCAACATATGAGATTTTTCCTGTACGCCCAACATCGTTAATAATTTCTAGTAATTGTGAGCTAACAACATTAGCTGGAAATTTATAAGCAATTGCTCATTTAGGAAATTTAGAAGTATAACCAATCTCATCATAAACTTCTAAATTATTAATTTTAATAACAATACCATCAATCATATATGAAAGTTTATCACGCACTTTAGTATAACAATCAATTTGATTAATAACTTCATTAATTGTATCGACTTTAACAATTAAATCACTAACTTTAAATTTATTATCTCTTAAAAATTGAATACTTTCATAATGCGTTTTAATTCCTAGTTCTTGTGCATTAACCAAATAATACATAAAAGCACTTAATTTACGTTCTGCAACAATTGAATTATCTAGACTTCTTAATGTTCCAGCAGCAGCATTTCTTGGATTAGCAAATTTTTTATCACTTTCTTCTAAATTATCATTCATTTCATAAAAATCATGAAAAGCCATATAGACTTCACCACGAACTTCAATTTCGTAGTCTTTATATTTTTGATCAATATAGTGAGGAATGTCTTTAATCGTTAAAATATTGGAAGTAACATCTTCGCCATTAATCCCATCTCCTCTTGTAATTGCCTGGTGTAATTTGGCATTTTTATAAATAATAGAAATGCTTAAACCATCAATTTTAGGTTCAACTACGTACCCTTTTAAATCGACACTAGCATTTTGATTATCTTGATCAAATTTTTTTAAATCATCATCATTAAAAGCATTTGAAAGTGATAACATTGGTCTTTTATGTTTTACTTTATTAAATTTTTCTGAAACAAAGCCGCCAACCTTTACACTTGGTGAATTGTTAGTTTTAAATTGAGGATAATTATTTTCAAGTTCGATTAACTCCCGCATTGTTTGATCATAAACATGGTCTGGCACACTTGGATCATCTAAAACATAATATTCATAGTTTCATTGGTCTAGTTTTTGTTTTAATTCGTCAATTTTAGCTTTTATTTTATCCATACGTAAAGTAACCTTTTTAAATCTTTATTAGTATAAATTTTAATTAAAAAACTAATATTAAAATAAACATAATTATATATTTATTATTTGCTTTTTACACTTTGCTTAATGGCTTCTAATTCAACAATCATATCGCGATATTGAGCAGCAACTTCATATTCACGTTTTTTTGCAGCTTCTTCCATTTTTTTCTTTAATTCTTTGATTTTTTGATTGTACTCGTTTTTTGAAGCTTTTGCAGCATATAATTCCGCATTTTGTTTATAATCATGGCCACTTAAATCTTCGTGAATTGGTTTAATAATTGTTTTAGGGATAATATTATGTTTTAAATTAAAAGCTAATTGAATTTCACGTCGTCGTTTTGTTTCATTGATTGCGGTTTGCATGGCTTGTGTTATTACATCAGCATACATAATTACTTTACCATCAGCATTACGTGCTGCACGTCCAATAATTTGAATTAAAGATTTATCACTTCTAAAAAAACCAGGTTTATCAGCATCAAAAATAAAAACACCTGCAACTTCTGGAACATCTAATCCTTCTCTTAAAAGATTAATTCCAACAACACAATCATAAATTCCTTTTCTTAATTTATTGATAATAACACTACGCTCTAGTGTTTTTAATTCATTATGTAAATAAGCAGCTTTAATTTTTGTATTATTTAAATAATTTGTTAAATCCTCAGCCATTCTAATTGTCATAACAGTAATAAATGCTCGTTGATTCTTGTTTTTTAAAAGCATCAATTCATTAATTAAATCATCAATTTGGTGTTCTGTTTTTCTAATTTCAATAATTGGATCTAATAATCCTGTAGGACGAACAATTTGACTAACTATATGATTATCTGATAACGCAATTTCTTCATCGTTTGGTGTGGCAGAAACATAAATCGTTTTGGAAAGTTTTTTATTAAATTCATCATAATTTAAAGGCCGATTATCTAAAGCGCTAGGTAAACGAAATCCGTATTCAACTAATGTTTGTTTACGACTACGGTCTGTATTATGCATCCCTTTCACTTGTGGTAATGAAATATGTGATTCATCTACAATTAATAATCAATCTTGATTGTTATATGAAAAAAAATCAAAAATTGTCCATGGTGTTTGAAAAGGTTCACGATGTTCAAGATGACGATAATAATTTTCAACTCCACTACAAAAACCAAACTCTACAATACTTTCAAGATCTTGTTTAGTTCGTTGCTCAATCCGTTGCGCCTCAATTAATTGATTGTTTTTTAGAAAAAATTTAACTCGTTCATCTAGTTCATTTTTAATATTTTCAATCGCTATTGCTAAACGCGAATCATCAAAAATATATTCACTTGCACTTCGTAAAATAAACCTTTGTTCTTTAGAAATAATATTATTAGTAATTGGATCGCAAAGATCAATTGATTCAACTTCATTTCCAAACATTGATATTCTTATTTTAAATTTATCACTATATCCAGACATAATTTCAATTAAATCACCACGAATCCTAAATGTTCCTGGCACTAAATCATAATCTTTTCGTTCATAACCTAATTGAACTAATTTATATTTAATTTGTTCAAATTCGATTAATTCACCAACATGTAAATATAAATTCTTTTTAGTAAATTCTATAGGAGAAACAGATGCATAAATACATGCAACAGAAGCAACAACAATGACATCATTTCTTGTAGCCAATGAATTAATAGTTGATAAACGCATCATCTCAATTTCTGCATTTGTTACTGAATCTTTTTCAATATAAGTATCAGTTATAGGTTTATAAGCTTCTGGTTGATAATAATCAAAATATGAAACAAAATATTCAACTTTATTATGAGGAAACATTTCTTTAAGTTCAGCAAATAATTGTGCAGCTAATGTTTTATTATGAGCAAGAACTAAAGTCTTTAATTGGGTTTTTGCAATTACATTAGCCACACTAAATGTTTTTCCTGTTCCTGTTGCCCCTAATAATACTTGTCGTTGAATACCTTGATTAATATTATTAACCATTTCATCAATTGCCTTTTGTTGGTCACCAGCTGGTTTATAATCGCTTATTAATTCAAATTTTTTAAAATCATCCATTGTTAATTATTATTTTTATCATCTTCATTATCTGATTGATTATCATCAAATGATTCTTGAATTGTTTGATCAGTTGGTATTGATTCGTTGAAATTTTCAAAATCAATTGAATTATTATTTTCATTAATTTCATTATCATTTACAATAAATTCACTATTTAAATCACTATAATCTTGATGAACATAATTTATTTTTCCTATTGAACATACTGTTTTTCAAGCAACATTATGTTTTAATTTTTCAAATAATTTATCTGAGTCTTCAACATAAATATTTAATGGTGCTTTTTGTTCATAAGCACGTAAATTAACTCCCTCACGAATTTTAGAAGTAATATCTAAATGTTTAGTTCATTCTTCATCAATATTACTAATCATAATTTCTGATAAGATTTGATTAAAAATTGTCGGTGTCATTAGTTGTTCTTTTTGCTCAAAAAAATTAATACAGTCTTTTTTTAATTTAGTTTTAATTTGTTCAAACTTAAGCCCATAATATTCGTCCTGATTTAAATAATTGTCATAAAAAATGTTTTGAGTTGCTAAATCAATTAATTTTTTTATGTCAATAATGTCTTCATTTGGTTTATTGTGTGATTGATAAATTAAATCATCAATTACACTATCTAACATTCGATATAAAATATCTTTATTATCTGATGAAATAAGAATTTTATCCCGTTGTTTGTAAATTAATTCGCGTTGATTACTTAAAACATGATCATAATCAATTAGATTTTTTCTTGTGTCATAGTTTAACGACTCAACTTTTTTTTGTGTATTATTTAGCAATCGTGTAAAGAATTTTGTTGAAATAACATCTTCACTTAATTTATCATCAGCTTTTGCTAAATTATCGGTTGCAAAACGACTAAATAAAGTATCTTCCATTGAAATAAAAAAACGTGATTCGCCAATATCACCTTGTCGTCCAGCACGTCCTCTTAACTGATTATCAATTCTACGTGAATCACTTCGTTCGGTTCCAATAACACATAATCCTCCAGCTTCTAATGATTCTGGAGATAATTTAATATCAGTCCCACGGCCTGCCATATTTGTTGCTATTGTAACTGCTTTATACTCTCCTGCACAAGCAATAATTTCAGCTTCGCGTGCGTGATTTTTAGCATTTAAAACTTCGTGAGGAATATTGACTCGTTCTAGTAATTGATGTAAAATTTCTGAATCTTCAACACTAGCAGTTCCAATTAAAATTGGTTGGCCATTTTCATGACGACGAATAACATCAGCAATTACATATTTCCATTTTGTTCTTTTATTATCAAAAACATAATCAGGATGGTCAATTCTTTTAATTGGTTTATTTGTTGGGATTGTAACAACAACCATATTATAAATATTTAAAAATTCTTCTGCTTCAGTAATAGCTGTTCCTGAAACTGCTGCTAATTTTTTATATAAACGAAATAATGATTGATAAGTGATTGTGGCAACTGTTAAATTTTCGGGTTCAATTTTAATTTGTTCTTTAGCTTGAATTGCTTGTTGTAATCCAGCGCTATAACTTCTTCCTTCCATAATTCTACCAGTAGATTGATCTACTAAAGCAATTATATCCTCACCATCATCATCTTTTTTAACAATATATTCGCGTCCATTAAAAAAAGTAAAATTTGCTCTTAAAGCATTAGTAACTTTGTGGATAATATCTGAATTTTCAAAATTATAATAATTTTTAGTATTGAAAAACTTTTGTGCTTTTTCAACGCCACTTTCTGTTAATGCTACTGATTGTGATTCTGGATCTAAAGTAAAATCTTGTGGATATAATGTTTTTACAAAACGATCAGCTTTAACATAATTTCCAATTTCTTCTTGAGGTTCACCAGAAATGATTAAAGGAGTCCGAGCTTCATCAATTAAAACAGAATCACCTTCATCAACAATAGCAAATCATAAGCCCCGTTGAACTTTATTAGCATAATTAGTTACCATATTATCACGTAAATAATCAAAGCCTAATTCTGAATTAGTTGTATATGTGATATCACAATTATAATTATTTCGTTTTTCATACTCATTCATATTAGCTGTAATTTGGCCAACAGACATATTTAAAAAATTTAAAACAGGTGTTGCAAATTCTGCTCCAACTTTAACTAGATATTCGTTAACAGTTACCATATGAACACCTTTTTTATAAAGTGCATTTAAATAAGCAACTAAAACTAAAGTTAATGTTTTACCCTCACCAGTCATCATTTCAGCAAAATCACCAAAATAAACAATAATTGCACCAATAATTTGAACTTTAAAAGCTCTTTTATTATGAACTCGATAAATCACTTCACGAATAATGCATAATGCTTCTACTAATTTATCATCTAATGGGTTATTATTTGCTAAATATTCAATAATATCATCAGATTTATTAATTAATTCTTGATCGCTAAAATGTTCGTATTCTTCTTCTTTTTTTAAAACTTCTTCAGCAATCAATCGAGCGCGATTTAAGATTCGGTTTTGGGGGGATATTTTTGATATTAAATTCATTTAAATCTAAGTCCTCTTATTGTTCATCTCATTTTAATTCGTCAACTGATTTTGGATGATCATTACGAATTAATTCATTAATATTACCATCTTTAACCTTAATAACTAAAGTTCCAAGTTCAGCAAAAATTGGGTTGTGGGTAACAATAATTACCGTTGTCTTTAATTCCTGGTTAACTTTTACAAATTCTTTTAAAATAATTTTTGACATTGTTTCATCAACAGCACCAGTTGGTTCATCGCCAAATAAAATAATAGGATTTTTAGCTAAAGCACGGGCTATTGAGACTCGTTGTTGCTGACCACCAGATAATTCATGTGGAAATTTTTTTGCGTAATCAAGCATCCCTACTGCTTTTAATGCATCTTGTGGTTTAATACGGCGTTTTTTATCAACTTGTAAATCAGCACCAATCTCTACATTTTCCTCTACAGATAGTGTTGGTAATAATCCATATTGTTGAAAAACAAATCCAATATATTCTTTACGAAAATCAGTTAATTTATTTTGATTCATATTAATTAGTTGTTTATCACAAACACGAACATCACCATCAGTTGCACGATCAAGTCCCGACATAATGTTCATTAATGTTGTTTTACCAGAACCTGATGGTCCTAAAATAATTACAAATTCACCTTTACGAATATCTAAATTAACACCTTTGAGTGTTTCATTAATTAAATAGCCATTTGTGAAAATTTTTTTAACATCACGAAGTTCAATAATATTATTTGGATCACGTGATGGCATATCAATGTCACCTTTGTGATTTCTGTTTAATTTTTCAACTTCTTTAATTCGTTTTTTAAGTTCTTTACGCTCATCTTTTGATAAGTCTTTTATATTAATTTTTTTGTTCTCATCTGGATTAGATAATGGATTAATAACATTATCATTTTCTTTTTTATTTCTCATAAAGCCATCCTTTATTTAAGTAAAGCTATTTAACATTTAATTATAAAACAAATCAATAATTTAATTTGATTTAAAGGTAATATAGTATTAAAATATATAATTATAAATCAAGTAATTAATTAAGGAAAAGATAGATCTATGCATAGTTTAATTAAGGATTTAAAAGCACGAAACTTAATCAATAATATAACTAATGAAGAAAAATTAATAAAAGCTTTAGAGAAAAATAAAGGAATTTATGTAGGTTTTGATCCTTCAGCTGATAGTTTACACTTAGGTAATTATATTATGATAATGTTATTAAAACGCTTTCGTTTATATAACATTAAAACATTAGCGCTTGTTGGTGGTGCTACAGGAATGATTGGTGATCCAAGTGGTAAATCCGCTGAACGTAATTTATTAGATAAAACAATTTTAGAAAAAAATATTGCAAAAATAAAATTTCAACTTGAAAAATTTACTAATTCTCAAGTTATTAATAATTATGTTTTTTATGAAAATATGACTTTTTTAGATTTTTTGCGTGATGTTGGAAAGTTAATCAACATTAATTATTTATTAGAAAAAGAAATTATTAATTCTCGTTTATCTGTTGGTATTTCATATACAGAATTTAGTTATAATTTGCTACAAGGTTATGATTTTTTACAATTATATAAAAATGATAACATAGCCATTCAAGCTGGTGGTAGTGATCAATGGGGTAACATTACAACTGGAATTGAAATGATTCGAAAAAATTTAGGTGATGACAATATCGCTTGTGGATTGACAATTAATTTATTAACAAATTCAGAAGGTAAAAAATTTGGTAAAAGTGAAAAAGGTGCTATTTATTTAGACGAAAATAAATCAACTGTTTATGAAATGTATCAGTTTTTAATTAATCAAAGCGATGCTGATGTAGAAAAATTACTTAATTTTTTAACATTAATTGATGTTGAAGAAATAAAAAAAATAATGCAAGCTCATAAAAATAATCCCGCTTTACGAGTTGCACAAAAAGCATTGGCTAAAGCTGTTGTTGTTGATATACATGGACAACAAAAATATGAACAAGCATTACATATTAGCGAAGTACTATTTAATGGTAGTATTAGTACATTAAATCAAGAAGAATTAGAAATCGCAATTAAGAGTTTACCTGCAACAAAATTAGACAAAGATGAAATTAAAATAATTGATTTATTAAATTTAGCTAATATTTCATCAAGTAACCGTATAGCACGAGATTTTTTAAATACAGGATCAATTTTAATTAATGATATTAAAATAAATGATGAAAATTTTTTAGTCAAGAAACAAGATGCTATTAATCAAAAATTTAGTATTATTAGAAAAGGTAAACGAAATTATTTTTTAATTTTATGAAATAAAGATTAAAAAATTTAATCAATTTTATTTATAATATATATGTCATTACAATATTAAGTAGCGAACCTTGTCAGGCCAGAGATGGAGCAGCAATAGCAATATCTTTTTATGTGTGATGACATATATTAATGTAATAAAACTAGCATTTTGCTAGTTTTTTTCATTTTATTAAGTTATTTATAATAATAAATTTTATAATTAAGAACGAATATTAAAATTATATATACAAGTTAAATGATTTAAGGAGTTAAAAATGCATAAAATAATTAATCACCCATTAATTAAAGATAAACTAACACGTATACGAAAAGTTTCAACAGTTTCTACTGTTTTTCGTACAAATTTAGAAGAACTTACACAATTGATGGTTTATGAAGCAACAAAAGATTTAGAGTTAAATGAAATAGAAATTGAAACTCCTGTTGTTAAGGTTGCAAAAGGTTATAAATTAAAAAATAAAATTTGTTTAATACCTATTTTGCGCGCTGGAATTGGAATGGTTGATGGTGTTAAAAGTTTAATTCCAACAGCCACAATCGGTCATATCGGTTTATATCGTAATGAACAAACACTAAAACCAGTAGAATATTTTAAAAAATTTCCTAAAAATATTTCTGAATCAGATGTTATTATTTTAGATCCAATGTTAGCAACAGGAGGTAGTGTTGTTGAAGCTGTTAATATTATTAAAAAATACAATCCAAAATCCATTAAATTTGTATGTATTGTTGCAGCTCCAGAAGGTCTTAAATATGTTCAAGAAGTTCATCCTGAAGTTGATGTTTATATCGCAGCATTAGATGATAAATTAAATGAAAATGGATATATCACTCCTGGTTTAGGTGATGCTGGTGATCGAATTTTCGGTACTAAATAATTTTAATAATTAAGTAAAGAATAAAAAAAGCAATTCATAATAGAATTGCTTTTTTATTTATTAATGGAGCAGATGACGAGAATCGAACTCGCGTCTCGACCTTGGCAAGGTTGCGTTCTACCATTGAACTACATCTGCAACTAGCACTTATATATTATATAATAAACTAGTCTTTTTTGTGCCATTTTTGTTCATTAATATAAAAAGTGGTTAATTTGTGTTTCTAGTTCATCAATTGTTTTAACAATAAGAGGTTTTAAATTAATAATTGTTTCCATGTGCTTTTGATATTGTGGTTCTCAATTAACTAAAATAAAATGACAATTAGCATTTATAGCTGCTTTTAAATCAGCTATACTATCACCAATCAATAAAGTATTTTTATGATCAACATTACAGTTGTTCATAATTTTTAGTAAACCACTAGGATCTGGCTTCATTTGTTCGACATCTTCAGCACCAAAAATATAATCAAAATAAGGTCGTAAATTATAATAATCAACCATTGTTGCTAATAAAGAAGATCGTTTATTTGATAAAATTGCTAATTTAACATTATTATTAAGTGATTTTAATTTTTTGATTAGGTCTAAAATACCATCAAAAAAATAACTATGGATCATAAAAAGCGAAGATTCATATATTTCATAATATTTAGAAATCATCATTTCAATTTCGATATTTAAAAGATCGTGTTTAGCATACTTTTTAAAAATATTTACCATAGAAAGATTACCTAACAGATCGTTCATTTCTTTTAAACTTGGTATATGATGATTATAAAATTCTAAAACACCAATAATAGATAGGCTCATAGTTGTATGCGTGTGCATAATTGTACCATCAAAATCAAAAACAATCAGTTTTTTGGTCATAAATCTTTTATTTTTCCTTCTTATTTGAATAATAATTAACAATAAAATTATCATAAACATCTTGTTTATCTAAATTAAAATTATTATAATCAACTTTCATGTATAGATCACAATCATAATAATCTTTTAATACAGAAACAATTAAAACATCTGCATAAGCTAAAAAAATATCAAATAATTCCTTGCCGCCAATAATAAAAATATCCTCTTTTTTAGCATCGTTTAATACTTGTTGAAAATCATTTGTCACAAAATACGATTCGTCTTCTGGTGTTTGCATAATTGAAGAAAAAATGATATTTGTTCGATTATTTAATGGTTTTTGATCTAATGAATCAAAAGTGTTTTTACCCATTATAATTTTATGGTTTTTTGTTGTTTTATAAAAATGATCTAATTCTTCTTTTATATATCATGGTAATTTATTATTTTTTGATATACCACCATTCAATGTTTGACATCAAATTAACTTTAACATATTTAAATATAAAATCCTTAATCATAAATGATTGTCACATTTTGATTATATACTAATTTATTTAAATTAGACTGATATATATTAAAAAATATATTATTTAAGTATAAAAAAACCAACCTCTTAAATTTCAAGGTTGGTTTTAGCGTAGCGATGACCTATTTTACTTGCGCTATTTTCGGCTCTAGAGTGCTTGACTTCTGTGTTCGGGATGGGAACAGGTATTTCCACTCTGATATAATCACTACACAATTATGATCGACATCTGAAAACTAAATATTATCAACTATTCTTCTAAAAATTAACTAAGAACTTATATAAGTCCTCGAATTATTAGTATCGGTTAGCTAAACACGTCACCGTGCGTACACATCCGACCTATCAACGTTATAGTCTTTAACGATTCTTACTTCATTTAAGAAAGGGAAGATTAATCTTGAAGTTAGTTTCACACTTAGATGCTTTCAGCGTTTCTCTATTCCACACGTTGCTACCCTGCAGTGCCGCTGGCGCGACAACAGGATCACAAGAGGTGTGTCCATTCCGGTCCTCTCGTACTAGGAACAGCTCTTCTCAATCTTCCTACGGGCATGACAGATAGGGACCAACCTGTCTCACGACGGTTTGAACCCAACTCACGTATCTCTTTAATCGGCGAACAGCCGAACCCTTGGAACCTGCTTCAGCTCCAGGATGAGATGAGTCGACATCGAGGTGCCAAACAGTGCCGTCGATATGAACTCTTGGGCACTATCAGCCTGTTATCCCCAGAGTAGCTTTTATCCGTTGAGCGATGGCCATTCCATTCTGAGCCACCGGATCACTATGACCTAGTTTCCTACCTGTTCGACTTGTAAGTCTCACAGTCAAGCACCCTTATACCATTACACTCTATACACGATTTCCAACCGTGCTGAGGGTACCGTTGCGCGCCTCCGTTACCTTTTGGGAGGCGACCGCCCCAGTCAAACTGCCCACCTAACACTGTCCCCCAGCCAGATTCATGGCTGCAGGTTAGAGAAATCTTATCACAAGGGTAGTATTCCAACGTTGACTCCATATACCCTAGCGAGTATACTTCACAGTCTCCTACCTATGCTCTACAAGTAATAAAATTTCCCAATATTAAGCTACAGTAAAGCTTCATGGGGTCTTTCCGTCCAATCACGCCAAGAGGGCGTCTTCACCCTCACCAGGATTTCACCGAGTCTACAGTTGAGACAGTCAAGAGATGGTTACACCATTCAAGCGGGACGGAATTTACCCGACAAGGAATTTCGCTACCTTAGGACCGTTATAGTTACGGCCGCCATTCACTGGGGCTTCAGTTAAAAGCTTCGCTTGCGCTAACATTCTTCTTTAACCTTACAGCATTGGGCAGGTGTCACCCCATATACATCGTCTTACGACTTAGCATAGAGCTGTGTTTTTGTTAAACAGTCCCCCCTCGCTATTCACTGCGGCTCACCTTTTACAGCGAGCACCCCTTATTGCTAACGTACGGGGTTAATTTGCAGAGTTCCTTAACTGTAGTTAACTCGCTAACCTTAGGATATTCTCCTTGACCACGTGTGTTCGTTCTCGGTACGGACTACTAATTGTTTAACGCTAGAAGCTTTTCTTGGAAGCATGATGTATATGTCTTCGCTACTGCCCGAAGGGGTCGCTCGTATTCATAACTTGGTCTTAATAGTAAGCGGATTTGCCAACTTACTAACCTTGTTATTTAAACCGGAATCCAATAACCGGCACATAATAACCTTCTCCGTCACTCCATCACACAATTTGTAGGTACAGGAATATTAACCTGTTTTCCATCGACTACGCTTTTCAGCCTCGCCTTAGGATCCGACTTACCCTGGGAGGACGACCCTTGCCCAGGAACCCTTAGTCAATCGGTTTGAGAGATTCTCACTCTCATTCGTTACTCACGCCAGCATTCTTACTTGTATAAAGTCCAGTAGTCTTCACAGTCTACCTTCGCCCCATATACAACACTCGTCTACCGCTACATATATAATATGTAACCCATAACTTCGGTTATATGCTTAGCCCCGTTACATCTTCCGCGCAGAGTCTCTCGACAAGTGAGCTGTTACGCACTCTTTAAACGGTGGCTGCTTCTAAGCCAACATCCTTGCTGTTTATGAAACTCAACATCGTTTTCCACTTAGCATATATTAGGGACCTTAGTTAATGATCTGGGCTGTTTCCCTCGCGACAATGAAGCTTATCCCCCACTGTCTGACTGCTAGAATAGGTAATATGGTATTCAGAGTTTGATTCTATTCAGTACACCGAGGTGGCGCCATCATAGATTCAGTGCTTTACCCCCATATTCCCAATAATCTAACGCTATTCCTAAAAATATTTCGACGAGAACGAGCTATCACGGAGTTCGATTGGAATTTCACCACTATCCACAGGTCATCCCCTGTCGTTTCAACGAAAGTGGGTTCGGTCCTCCAGTGAATGTTACTTCACCTTCAACCTGCCCATGGATAGATCACCCCGTTTCGCGTCTATTATAACATACTAAAACGCCCTATTAAGACTCGGTTTCCCTACGGCTCCATCTCTACGATTTAACCTCGCATGCTATAATAACTCGCTGGCTCATACTTCAAAAGGCACGCTATCACACATTAATGTGCTCTAACACCTTGTAAGCTTATGGTTTCAGGTTCTATTTCACTCCCATCTCTGGGTTCTTTTCACCGTTCCCTCACGGTACTATGCTCTATCGGTGACAAACTAGTATTTAGGCTTACCCAATGGTCTGGGTGGATTCAGACAGGATTTCACGTGTCCCGCCCTACTCAGGATCCTACTAGGTAAAAGAATGATTTCGTATACGGGATTATCACCCTCTATGATTTGGCTTCCCAACCAATTCTACTATCATTCTCTATTCCATATTGTAGTCCTACAACCCCGAATCGAAATTCGGTTTGGCCTACTCCCCTTTCGCTCGCCGCTACACAGGGAATCACTTCGTTTTCTTTTCCTCTGGTTACTGAGATGTTTCACTTCACCAGGTATCGCTCTGTTAAACTATGAATTCATTTATACAGTAATCTAATTCTCATTAGATTGAGTTTCCTCATTCGGAGATCAACGGATTAAAGCCTCTTATCAGCTACCCGTTGCTTATCGCAGATTAGCACGTCCTTCATCGCCTGTTTGTCCCAAGGCATTCACCATAAGCTCTTAGTAACTTATTATAAATCCTTAAAGTTGTTTATTTATGATTGTTAACTATATTTAATTTAATATAGTTTCTGTTCACTGACAACTTAATGTCAATGATTAGAAAGATTTGTTGATAATTATTCAGTTTTCAAAGATCGATCATCCATAATAAATTAATTATTATGGGAGAGAATAAACTCTCAAAACTAAATAGAATCCGACCATATGAATTTTTAAAAATATTTAAAAAAGTGGCTTATATTCGTATGGATTTTAATAAAAATGTACACTTATTAGTACGTAAATTAAAAATTTACTCCGAAGAAAGGAGGTGATCCATCCCCACGTTCTCGTAGGGATACCTTGTTACGACTTAACTCCAGTCACCGATCCTACCCTAGACGCATGCCTCCAAAAGGTTAGCTTTGCGGTTTTAGATATTACCAGCTCCCATAGTTTGACGGGCGGTGTGTACAAGACCCGAGAACGTATTCACCGCGACATGTCTGATTCGCGATTACTAGTGATTCCAACTTCAAGAGGACGAATTGCAGCCCTCTATCCGAACTGAGACTAACTTTTTCTGTTTCGCTTCATCTTACGATTTTGCAGCAGTTTGTATTAGCCATTGTAGCACGTTTGCAGCCCTAGATATAAGGGGCATGATGATTTGACGTCATCCCCACCTTCCTCTACCTTGCGGTAGCAGTATCGCTAGAAAAGTAACTAACGAAAGGGGTTGCGCTCGTTGCGGGACTTAACCCAACATCTCACGACACGAGCTGACGACAACCATGCACCACCTGTCATATTGTTAACCTCAACTATATTTCTATAGCATCGCAATAGATGTCAAACCTAGGTAAGGTTCTACGTGTATTGTCAAATTAAGCAACATGCTCCACCACTTGTGCGGGTCCCCGTCAATTCCGTTTGAGTTTCATTCTTGCGAATGTACTACCCAGGCACATCATTTAATGCGTTAGCTACAACACCGACCCATTCGGGCCGACATTTAATGATGATCGTTTACGGTGTGGACTACTAGGGTATCTAATCCTATTTGCTCCCCACACTTTCGAGCCTAAGCGTCAGTGATAGTCCAAGTTGGCGCCTTCGCCACCGGTGTTCTTCCATATATCTACGCATTTTACCGCTCCACATGGAGTTCCCCAACTCCCTACTACACTCTAGGTTTACAGTTTTTGATACAGCTAGACGTTAAGCATCTAGATTTAATACCAAACTTACAAACCCGCCTGCGCTCGTTTTACGCCCAGTAAATCCGGATAACGCTTGCATCCTATGTATTACCGCGGCTGCTGGCACATAGTTAGCCGATACTTATTCAAATGGTACAGTCAAACTAAAATCATTTCCTATCTTAGCGTTTCTTCCCATATAAAAGAACTTTACAATCTATAAGACCTTCATCGTTCACGCGGCATTGCTTCATAAGGCTTGCGCCCATTGTGAAAAATTCCCTACTGCTGCCTCCCGTAGGAGTATGGGCCGTGTCTCAGTCCCATTGTGGCTGTTCTACCTCTCAGTACAGCTACGCGTCATTGACTTGGTGAGCCATTACCTCACCAACTATCTGATACGTCGCACCCTCATCCAAAAGCGTCGCCACAAAGAGCGACTTTCTACATCTTCTCATGCGATATTGATGTTATTATTCGGTATTAGCTAATCTTTCGACTAGTTATCCCCAACTTAAGGGTAGATTGGATACGTGTTACTCACCCGTTCACCACTAAGCCTAAAAGGCTTCGTTCGATTTGCATGTATTAGGCATGCCGCCAGCGTTAATCCTGAGCCAGGATCAAACTCTTTAAAATTGACGGATTCTATTTAGTTTTCAAAGAACATTCTTATTGTGATTAATTATTAATAATCTTTAAACACAATACATTATAGTATAAACAATTTTTTTTAAAATGTCAAGAAATATTTTATTTTTTTAAATTATTTAACTAATTGCTTAAAAGCACTAGAAGATTATATTAAAAAAGTGTAGAAAATGTCAAGATATTTATTTTAAATTTTAATTAAGTATTTTTATATTTTTAAATTCTTAATTCAATATAGATTTTTTATTTTAAAAATCAAATATTTTTTATTCTAAATTCTTTATTCTCTAAATAATTTAATTCTTCACATTGATATTTCTTTAAATTAATAAATTTGATCTTATATGATACTAAACATTGATACATATAATTTTGATTCTTATCAATATCTTTAGTGATGTATTTTTGTTCACCAACAATTGGTGCATGCAAAAAATTTAAAACTGCTCGTATTTGGTGGGTTCTTCCAGTTAATAGTTCAATGTCATAAATAAAATATTTTTTATACTTATCTATATATGTATATTTGGTAATTGCTGTTTTATTAAATTTTGTTTTAAAATTTTGTACAATCATTGTGTTATCATCAGCTTTATACATGAAATTATAAATAACATCATTATTTTTAGGTAATTGATTGTATGTTAAACATTTATAAATTCGAATCACATCATTATTTTTAAACATTTCAGTAATTACTCTTAAAGCATTTGCAGTTTTAGCTGCAACAATTAATCCACTTGTATTTAAATCTAAACGATGACATAAGCTAGGAACAAAAGCACTTTCATCAAAAGGATGATATTCATCCTTTTTAACTAAATATTTCTTAATTCTATTTTGTAAAGTGTCATTTTTATATCTATTATCTTCATGAACAATTAAACCAATCGGTTTGTGAACAACTAAAATATTTTTATCTTCATATATAATATCTAATTCATCTTTAGCATTAGCAAAAGATAAGTCAACTTTTTTTTCTAAAAAATTCTCCTTAGCATAAATACTAACAACATCATTTTCTTTTAATAAATAATTATGATCAACTTTCTTATGATTAACTTTAACATCTTTTGTTCTAATTATTTTATAAACAGCAATTCTTGATAAATTAGGAAAATTTTTTAATAAAAAACGATCTAAACGTTGATTGGCATCGTTAATTTTAATTTTAATTTCTTTTAAATTCATTACTTAATTTTAAATCCTTAGTTTTAATTATCTTTTTCTTCTTGAGAAAATATATTTTGAAAAATTTTTAAATATTCATGTACAAAATGATGAATTATTGGGTTTTTAAAAAGTATTTCACAAATTATTTGAGCTTTTATGACAAAATTCTCAGGTTTAATTTCTTTTTTAAGATTTAAATTTAAAACTTTAATAACCTTTCTTTTATTAGTCTGTGTTTTATAAAATTTTAAGAATTTTTCACTTTGTTTTATTGATAGTTTTTTTATACTAAAAACAGCACTTTTACTTAAATAATTAAAAAAGATGTTTGCTAATTTAACTTTCATAAAATTAGATTTGGAAGTTAGTGAAGAAATTGTTTGCAATTCTAAACTACTATCATAAATTGGTTTTGAATAAAAATGATGATCTTTTTTATAAACAACAAAGTCTAAATTAAATTTATTTGAGAAATCAAATTCTTTAATATACATTACATACTTAATTTCTTTTTTAATAATTCCCCTGTTTTTTTCTTGTTCATTAGTAACAAAATATGAAGAAGAAATAATTTTTGGCAAAAATTCATAATAACGATACATTTCATATAATTCTTCGTTCGTTTTATGACGAATACACATAAAAATTTCTTTTAAAATAAAAGCATCATATAGCGCATTATGTAATTTGAAAAAATTAATTTTAATTCCACTTAGAATTCCTAATGAAAATAAACTAGGAACTGATTTAGTTTGTAATTTTTTTTCAAAAAACTTATAAACATCAAAATAAACGATTTTTTCAAAATCGAAATTATAATATGTAAAAAGCTCGTGCATTTTTTTAATATCAAAATCTGTGAAAGTAATGACAATCGCATTTTTAACAAAACAATAAATTTCTCTTGCTGCTTGCTTATCTAATTTTGGCATTTGTTCAATATTATTAAAATTTTTATTAAAGTTCAGCAAGGTTTTAATACGTGAATTAATTTCTTGTTCAGGATTGAATTTTTTATTAAAATTAAAAATTTTATTTTTTTTAATATTTAAACGGTATCCGCTAAACTGAATAATTCGTTGTTCGCCCTTACTATCTGTTGCTTCAATATCAATAAAGACTAAATCCTGATCATCATTTATTTCACTAATATTATCACATATTTTCATCACTATCTCTTTTACTATTATTAACTTATCTGTGAAAAATACCAAGTTTTAGACTTGGTATTTTATTTAACAAATGATTTTCGATTTAAATCAACAATTCTTGATTTACCATCATCAAAGAATTTACTTAAAGCACGAATTCCATTTGTTTTTGCATAGAATAATTCTTCATCATCAATAATTAAAATTTGATAAAAACTTACAAAAAAATCATTATTAATTTTACACTCTAAAGAATTCTCGCCATATCAAGATAAATCTAAAAACCCAGCAATTTTATTTGATTTATTAAAATAAGAGGGATTAATGAAGGTTTGTAATCATTTTAGTGTTAAATGATTTGAATAAGAATATTTAACGATATTAATTAAAAAATGAACTGGTCACATGTTTTCATGTTTATTAAAATCTCAATCAACAGGTAATTCTAAAAAAATCTCAGCACGCTCTTGCGTATTTTCTTCGTGATTATGTGATTTAAAAGCACCTAGTCCAAAAGTCATTAATATTTGTAAGTTTTGTTTTTTAATAACTAAAATATCATATTCAATCGGAACACGTATATGTTCATTTGATAAAAATATAATGTGGTCCACTTGCCCATATTCTCTTAAAATATAGTTATAAATAATTTGTTTTTCTTCAGCACTATATTTATAATTCATAACTACCCCATTATAAAATTCTTATATATTAATTATATAATAAACTAACGTTTATATTGAAAAATGAGAACCTTTTAACAACCTTAAATTAATATTTTTAAATCTTTTCACGGAAGGCAAGGTATGCTAATTTTAAATTAGAAATTGATAAATCATTTGTGGGAAATTCTAAAGTATTAATATTTAAACGAACATTAAAAATTTTTCTTAATGTCGCGTTTAAAGCAGCGTGTTCTTCAACCTCTTCATTGATGTTATTAAAAATAGCTTCTAATTTTAATAAAAGATAATTATTAAAAACATGTGATTCAATTTTAATATTTAAATTATTTAAACACTCATATATTAAATTTTTAATCGGTTTTTGTTCGTATATATCATTAATTAACGTAGGATTAAAAATAAATTTTTTATCTTTTTGATAAGTATTATAAATGTGATTAATAATTTCATTTAATAATGAAATATATTTATGTTTACGTTGGCGCTCTTTTTTTGTTTTATATTTTGTATCAATTTCAAAGATTTCAACCATTTTTTGTCTTAATAAATCACTTTCAAAACCAGTTTTTCCATAAAATCCATTGTTTTCATCTAAAACATAATCAGCAATTGTATTTAGTGTTGGCGAATATTGAACATAATCAAAATTACGCATTGGCGTATACAAATATCCACTATCTTCTAAAACGTCACCAACTTCATTTTGATCATCTAAACTATTTTCGTAATCAGCAATTTCTTTTGGTGTTTTATTACGATGAATTTTCAAACCATAGTCTAAAATTTTAGATTCTCCATCTGTGTTTTTTTCATTAAGACCAGCAAAACATAATTCATTTAAATATTCATAGGCTGCTTGTTCGTTTTGTTTATTAATTGAGTTACTAATTGTAAAACCATCTAAAACACTCATCGTATCTTTAGGTCGTACAACATGAAAATTATCTGATGTTGGCTTCATGTTTGCATAATTTGAATCAGCTTCAGTTGTATACTCGCCCCCATTAGCAGCAAAAGCAATATCGCCACTATAAGCAATTGCTCCAGCAACATTAGCCATTGCTAAATCATTTAATACATCGTTTGAATTTGCTTTTAAATACATAGAGTTAGGATGTAGATTTTTAAACATATTACTAATATTGTTTAATTTATCTTCAATATAAGATACACTACTACTATCTGGTAAGAACACATTCGTTTTTAATTCTTTTTGTCTATCTTCTAATTGTTTTTGCAAACGCTTTAATTTTTTAATATTTTCTTCTTTAATATCTTCTTTTATATTCGGATTTTTTAAAATATGTAAAATTTGACTAATACTATTATTAATTCCTAGAATTTCATGATTAATAATTTTAATAATGTTATTTTTATTATCCCCTTCTAATTGCATAATTTTAGCAATATCATAAATTGTTCTCGCATCATCAATAGCTATAATTTTTGTTTTATTTTTAGCAATTGTAATCGGAGTTCTTTTATCTACACCAAATCCTAAATTAGCTTTTTGTTCATATAAAAAACGATTGGGTGTGCCATCATTACGTGTTAATTCTTTAAAAACATCACTTCAATAAACATCTTTTTTATTATCATCCAAATTAGGAATTTTTTTACCACGATAAGCAAAAATTAAATCTTGGTAAAAATAAGGAATCATATATTCTAATAATTGGTCATTTTTACCATCCTTATCTAAATCTCCTAAATATGATGAATAAGCATTTGAAATTGCTCAAACTTGCTTTGTGAAAATTTTTTTTAAACCTTCAATTGAAGTAATTGTTTGGTTATTTTCATCTTTCAAATTAAATTTTGCTCAATTAATTTTCTTAATTAATCCATTTTTTGCTAATTTAGCTACTTCATATGCTGAAGCAACTTGTACATCTGAAATTTTTGCTTTAATTAATGAACTTAAAATTTCATTATTATCATAAAAACTATATGATAAATTATTTTTACGAAATCGTTTTGCTTGCTCATTAGCAACATCTGGATCCATATAAGAATCAAAATTACCAACTACAAGTTTTGATTTTTGACTACAAGCTGTAATTATAGGTATAAAAACTAACAAACTAGTAATTCCAGCAAAAATTTTTAATAATTTTTTATTAATTTTCATAATTTAAACCTGTCTATTTCAAATTTTTAATTTGCGTTTATTAGTTTGTTTATTAATTAATTTTAACTTAATAATTTTATTAGCACTAATTAATGCAGCAATTAAAATTGTTAATAAAACCATTAAAGCACCAAAACAAACCGCTCATGCTTTAATTCCTTTAGCCATCGAATACATTTCTGTTCCAATGGTATTAACTTTACCTCCAACCAATTTAGTAATAATAAAATCATCAAAAGACATAGCAAAAACAATGATTGTTGCTGAAATAATTGAAGGTAATAAATAAGGTAAAATAATTCTAAAAAAGGTTGCAATTTTTGTATAACCTAAATCATAACTAGCAAGAATTAAATTTTTTTTCATTTTTAACATTCTAGGATAAATAATCATAATTGCATAAGGAGTGCAAGAAGAAATGTGTGCTAATATAATCGTACCAAATCCAAAACTAAAACCAATTGGAATAAAAGTAGCAGCAAATAATAAAGATAATGAAATACCACTAATTACGTCTGGAATCATTATGTTTGTTTGTGATGATGCTGTCATTAATTTTTTATAAAATATTTTTGCATGCCAAACCCCAAAACAAGTTATTGTTGCAATGATTGTAGAAATTGGTACAGCGATAACTGAAATAATAATTGTATTAATTAAAGCATCTGTAAATTGATTGGTTGTTAGTGTTAAATAATTTGAACCATCATTCCAGTTAAAAGCTGATGTAATGTTTCCTTTAATTGAAGGTGTGGTAAATGATAATAAAACAATGATAATTAAAGGAATATAAATGATTGCTAAAATAATTCAAATATAAAAATTACGCAATCAAGACAATCAATTAATCTGCTTCATTATTCTTTACTCCTTTTTTTAGATTTTTTCGTTTGTAATAAATTAATTGGACTATTTTTGGAATAATAACAATAATAAGATAAATCCCTAATAAAATTAAACTAATTACTAAAGTAATTGTTGATGACCGTGATAGTTGAACTCCACTTGAAATACCGTCTTGTCCTTGAGCAAAGATTTCTCCACCAATTAGTCCTCCAGCGTTATCATTATTTAAAAAACCTGAAACCACAACTGATGTAAATGCTGCTAAATAAACAATCCAAACTCCTGAAATTAAAGCATTTTTAGTGTAAGGAATTACAATATGAAAGAAGGTGTAAAAAAAACCTCTTCCTAAATCATAACTAGCATTAACTAGGTTTTTAGGCATTGTTGACAAAGATGTATAAATAGGCAAAATCATTAATGGCAAATTAATATAAACTAAACCTAAAATAGTAAAAATGTGCCCATGTGTACTATTAATTTCTCCATTTACTACATCAAAAAAGGTTTTAATACCAATTAGTTTTACTAACATACTCATCCAAATTGGTGAAGTAATTAGTGCAATTGCAATTGCTTTTAAAAATTTTGATTTACCAATACTTAAAAAATACGCAAATGGATAACCAATAATTAAACAAAAAATTGTAGCAACTGCAGCGATTCATAACGAATTAAAAATTTTCATTCCAATAGTTGGCGTAATTACTGACCAATTATCAATGATGTTTGTTGTTACACCAGTTGAATCAGTATTGTTAATAAAAGTTTTAACAATAATACTAACCAATGGTATTATCAATAAAAAAATAGTTACTAAAAAATAAGGAATTGTTAATATTAGTGCCTTATTCATTTTTAATTTATCAAAAAGAGTTTGTTTATAATGCTTACGATTGTTTTTTTTAATTACTTTAACTTCTTTTAAAAAAAATACTGTTTCGTTTTTTTTGTTAATATCTGATAATTTAGATGGTGCAAAATTGAAATTAAATTGCTTGTTCATTATTTAAATAATCCTTTTTAATTACATGCACATCAATGTCATCTCAATCTAAACCAATTTCATCACCTTCATTAACTTTATTAACACCTTCAACACTAATAATCATGTCATTATATTTACACTTAATATCTCACATTAACCCTTTATATAATACTGATTCAACTCTAGCATTAATTGATCCTTCGTCTTTTTTAACAACATCAAAGTCTTCTGGGCGAATCATAATATAACACGCTTCATTTTCGCTAAAACCATTAATCACATCTGTTTTAGAAGTAATACCATCAAATTCAACTTCACCTTTAGCAATATAATGACCTTCAAAAATATTAGTTTTACCAATAAAATTAGCTACTCACAATGAATTTGGTGAATCATAAATATCACTTGGTTTACCGACTTGTTCGATTTGACCATTTGACATAACCACAACTTTATCTGATAAACTTAAAGCCTCTTCTTGATCATGAGTTACTAAAATAAAGGTGATTCCTAAATTTTTATGTAGTTTTTTTAATTCATCTTGTAATTGTTTACGTACTTTTGCATCTAGTGCACTTAATGGTTCATCTAATAAAATAATTTCAGGCTGTATTACTAGTGAACGTGCTAGTGCAACACGTTGTTGCATACCACCCGATAAATCACTTGGATACGAATCCTTACGTGCACTTAAACCAACTAAATTAATTACACGATTTGCTTCTTTTTCGACTTCTTCTTTAGTTAATAATCGTGTAATATTTTTATTTTCAAATTGTTCTTTTTTTAATTGTGGATAAGTTGCTCAATATGATTCTCAATAATCAAGATCATTATATTTGTAAGTAATCTTATCATATCGTTTATCTAATCCACTTTTTTCTTTATATGCTTTTTTTAAATTAAAAATTTCTCTATCAATTGGATCGTTTAAATTCAATTTTTCATGACTAAATCAACTTTTAAAATAATTTAAAAAACCTGGTTTTCCATTTTTATTAATACCTTGTTTTTGATAAAGTTCATCAAGTGTGCCAAGATATTGGTTTTTTCGCATTTCTTTTATTTCAAAAATGTTCTTATTCTTTTGGTATTTAAGATCCATTTTTTTGATTTCAGCCAATAACCCGCGTCTTTGTTTTTTTAATTCTTTAATTTTTTCATTTGCTTTTTTAGAAGCTGAATTATAAACTTTATCAGCTTGCAAATAAATACTTTGATCAACATTATCTTTTGGTTTACGCATTAGTTTTAAACCATATTTTATGTTTTGTTTAACAGTCATATTAGGGAATAGCGCATAGTCTTGAAACACCGTTGATGTTGGTCTTAGTCGAATTGGCATATCCTTAATATCAATTCCATTATATAAAATTTTTCCATTAGTAGGTTGTTCAAATCCAGCAATAATTTTTAACATTGTTGTCTTACCACAACCACTTGGTCCTAATAAAGTGACAAATTCACCTTTTTTTATTTTTAAATCAAATTTGTTTACAGCAGCAAATCCATCATCATAGATTTTTGTAATATCTCTAAGATGTAGGAGTGTTTTTTCCATATATATCCTCCATAAATAAAAAAATAAAACATGAGTAAACTCATGATATTTCAAAATTGAAAACAAATAAAATATAACATAAAAAACACTAATAAAATTATTAAAAACAACTATTTTTTTTTAATTTGATTTTAAAGTAAAAATTTGCTTTGTTTTTAAGTTTTTTTTATATGAAAAATAGCTTAAATTTTAATAGTAAAAGCAAAGAAAAAACTCTTTTTTTAGAGTTTAATTTATTTTTTAATTATTTAAATTAAAATAATAATTTTGATTTAAAAACCAATAAATATTTCATATAGTAATTAATGATATATCAATCAATAAAGGTATAAAATTTCAAGCAGTATTTATTAAAAATAAGAAGCTAGCTTTATGACTAAAGTTAACTTCTTATTTTATTTATTAATAGATGTATTTTTTTAATACTTTAGGAATGTTGATACTACCATCTTCTTGTTGATAATTTTCAACAATTGCAGCCCATAATCGATCAATTGCTAATGATGATCCGTTTAATGTATGAACTAATTCGGTTGTTGCATCAATTGTTTCTTTATAACGAATTTTTGCACGACGCGCTTGGAAATTAGTGCAATTTGAACAACTTGAAATTTCTTTATAAGCGTTATATGATGGTAATCAAACTTCTAAATCATATGTTTTAGCTGAACTAAAACCTAAATCGCCTGTGCATAAAACGATTCTTCTATAAGGTAATTCTAAAGCTTCTAAAATACTTTCAGCCTGTCTTGTCATTGATTCTAATTCTTTAAAACTATCTTCTGGTTTACATAATTTTACTAATTCTACTTTATGGAATTGGTGTTGACGAATTACACCTCTAACATCGCGTCCTGCAGATCCTGCTTCACTACGAAAACAAGGTGTTAGAGCTGTATAGTATAAAGGTAAATCACTAGCTTTTAAAATTTCATTACGATGTAAATTTGTTAATTGCACTTCAGCAGTTGGTGATAAATAATAATTTGAATTTTCTAATTTGAATAAATCATCTGCAAATTTAGGTAAGTTTCCAGAACCAATTAAAGATTCTTGATTAACAATAACAGGTGCTCAAATTTCACTAAAACCTGCTTTTACATTCATATCTAAACAAAATTGTTGTAAAGCACGATATAAACGTGCACCAAAATTAGTATAAATAATAAAACGACTACCAGTAATTTTTGTTGATTTATCAAAATCAATTAACTTGTTTTTTACTGCTAAATCTCAATGCGCAAGGGGTAAAAAATCAAATTTTTTTGGTTTAAAAACTTTTTTAATTTCGACGTTGTCATTTTCATCATTGCCAATGGGCACTGAATCATCTGGCATATTAGGAATTACTAATAATAATTTATTTAATTCATCTTGTTTGTTTGATAATGTTAATGAAATATCTTGCAATTCGTTTTTAATATTATTAACTAAATCTAATGTTTGTTGTAATTTAGGATCTTTGTTTTTAGCTAAAATACCAATTTCTTTTGATAATTGATTTTTCTTAGCATTAAGATTTTGTTCTTGTTGCATTAAACTTCGTACTAAAACATCTAAATCAAAAATTTGATCAAACAAATTGCTTGGCACCTTTTTATTTAACATCTTTTCTTTAGTAGTTGCAATATCTTTTCGTATTAAATTAATATCAAACATATAACTCTTCCTCTTTAAGTTTGCTTAACAACCTATATAAACATATATTTTAATAAAATAAAATGCTATTTAACCAAACAAAAAGGTTAGCAAGCATTTTATTTAATAATTTAGTTAGATTTTTGATCTTTTTCTTTATCTTTTATTGATTCTTCATCGTCAACAACTTTTGCTTCTTCTACACTACTATTTGCTTGTTTTTGAGAAGCTTCACGTTTGTTTTTATTAGCAATTACCTCTGGTGGTAATTGTTTGTATTGGTGAATATAATCAATTTGTGGCTTTAAAATTGTTTCTAATTCTAATAATGTTTGAACGATTAATTCCAATTCTTCACGATGTTCATTAATCATTTCTTTAACAATTTTATATTCACGTTGAATAATTTTTTCAATTTCAATATCAATTGCTAATGCTGTTTGTTCTGAATAACTATTTCGATAAGGGTTATCAACACCTTCAGTAGCAATATATTGCGTTAAACCCACATCTGTCATTCCAAAAGAAGCTACCATTGCTCTTACGATGTTTGTAATTTTATAAAAGTCATTTGCTGCACCTGTTGTAATTTCATTAGGTCCATAAATTAACTCTTCTGCAGCTCGACCACCTAATGCAGTACGCACTCGACTAATTAATTCAGTTTTATTTTGTAAAATATTTTCATTTCTTTCAGGAGTTTGTAGTGTATAACCAGCAGCTCGACCACGAGCAACAATAGTAATTTTCTGAACAACATCAGCACCTGGTGAATATAAACCAATTAATGCATGTCCAGCTTCATGGTATGAAACTTGTTCACGTTCACGTTCAGAAATAACTTTATTAGGACGTGAAGGTCCTGCAATAACACGATCAATCGCTTCATCTAAATGATTCATATGAATTGATGTTGCTTTATCTCTAACTGATAGTAATGCGGCTTCATTTAAAACATTTTCTAATTGTGCACCACTAAATCCAGCAGTACGACGTGCAATATCTTCTAAACTAACTTTCGCAGATAAGTTTTTATTACGTGAGTGAATTCTTAAAATTTGTTCACGTTCTAAAATATCTGGTAAGTTAACACTAATTTGTCGGTCAAAACGTCCAGGACGTAAAATTGCGTCATCTAATGTGTCTAATCTATTTGTCGCAGCCATAACAATTACACCACTTGAAGTATCAAAACCATCTAATTCTGATAATAATTGGTTAATTGTTTGGTCTTGTACAGCAGTTAATGAATTACCACGTTTTTTAGCTACTGAGTCAATTTCATCAATAAAAATAATGGCGGGAGCACTCTTACGTGCTTTTTCAAATAATTCTCTCACACGACGTGCACCAACTCCAACAAAAGTATCTTCAAAAGATGAACCCGTTGTTTGAAAGAATGGAACATTAGCTTCACCAGCTACAGCTTTAGCAATTAATGTTTTTCCTGTTCCTGGAGGTCCATATAACATTACACCTTTTGGGATTCTAGCACCAGCAGCAACATATTTTTTAGGTTCTTTTAAGAAATCAACAATTTCAATTAATTCTTCTTTAACCTCAGCAATTCCAGCAACATCACTAAAGCGAACACCAGATTTTGTTAGTTTAGCATTCGCCTTATTAGTTCCTAATAAAGAGTCGCTTTGTGCTTTAGAAACACGTAAAATAATGTATAAGAAAATTATGAAGAAAATAATTGAAATTAATGGAGAAATAATAACTTGTGGATTAAAGCCAGAAGATGGAATTCCTGCATTTTGAGTAATAATAGATGTTAACCAATTTCATTGATTATTAGTATTCCCAGAAATTGGTGCATAGATATTTGTTTCACCAAGTTTAAACTTTTCAACACCTTCACGCACAATATTAAATGAGATTCTTGACAGCCCTAAACTTGATGCATTAGAAATTATTTGAAAATTAGTATTATTTAAAATATTAACACGTGCAGTAAGTAGTTGATCTTCATCAATTGTATAACGTGTTATTTGAGTGCTATTAGGCCTAATAGTATTTAAATATAAAACTTTTTTGTTATTAACAACTTCTGTTTGAGCACTTTTAACAATTACGTTATCATATTCTTTACGAACAGAAAAATAAATAATAACTCCAATAATTGTTCCTATAATTAATAAAATAATTAATCAAAATATAATTCTTCGATTTCTTCGTTTGCGCGCTTCTGGCGATTGTTTACGTGGTTGAGTTAAGTCGTCTTTTTTAACATTATTATCTTCTGATTCAATTTTTGAACTAAATAAATTTACTATTTTTTTTAAAAAATACATCTAATTACTCTCCATCCCCTTCGTTTTTATTATTTAAATCATTTTGATAAACTTCTTCTTTTAAAACACCAATATATGGTAAATTACGCATAATTTCTTTATAATCTAAACCAAAACCAACAATAAAGACTAATGGAATATCACAACATACAAAATCGGCTTGTAAATCTACTAATCGGCCGTCTTTTTTATCAACCAAGGTAACTAATTTAATTGAACGAGCGCCTCGAATTTTAAGTAAATCATAAACCTTTTTAATTGTGCGTCCTGTATCAACAATATCTTCTACTAAAATTAGATCACGATCTTTAACTTCAAATTTAAGATCAGTAATAATTTCTGGTTCTGTTTGAGCACTAGTTGCTCCCTTAAAACTACTAATTGCTAAAAAATCTAGTTTCATGTCAATTTTAATTTTTGGTAATAATTTGCCAATAAATGGAATGCATCCTTTTAAAATACCAATTATAATTGGTTCTTTACCTTCATATTCCTTATTGATTCAATTAGCTGCTTCAGTGATTTTTTGATCAATTTGTTCTTCAGTGATCAAAACCTCTTTTATTCTTGGATCAATATCTTTCATTTTTATACCATCATTTCTTGTTTTTTGATTTTTAATCATACTATTATACCATTAAGTAATTATTATTATTTAAAGTAATTAGGCATAAAATAGTTTATTTTAAACTCTTTTATTAAAATATCTGGTTTATTACTTACCTTTAATTTTCCATTTTTAAAAGCTTTATCAACAAAAGCTTTTAAAGTAATTGAACGATCTTTATTTTTAATCATTTCAACATAAATTTTAGTGTTTTCTAAATCCACATCCTCAAACATCACAACAGCTAATTCACTTAAATCATTATAAGTAAGTTTATCAAAATTTAATTTATTAACTAACTCTTGTAGTTTTTGTTTTTGTTCTTTTTTAGATTCATCAAAACTTAAATCTAATCGTGCATCAGTGTTTTTGTTAATATCATATATTTTTTTGTAATAAAACTTAAAATTAATTGGTGAACTATAAATTTCATTTGTTTTATTATTAATATGTAATATACTTAGTTCTATTATTTGTTTTTGTTTATCAATTTTAGTAATTTTATATTGACGACTAAAATCATTAAAATCTTGCTCTGGACAAATAAAATAAGTTCTAAAATAACGATCAAAATCGTTGATTGTAATTTCAGATAAAGGTTTTTTAAAATATAAAAAAGTTCCTAACTCTTTAATTTTATTTGAATCATCTAATATCCCAATTTCTGAATTATTTTTATGAATAATTTGATTTCTTTGTCGATCATATTCATCCGCTTCTTGTTGATTAATTGACGTTAAGGTTATTGGTTTAAATTTTTCGTTTTCTTTAAAAACTTTAATTTTAAAAATTTTTGCAATATTAGGGTTGTTGGGATTAATCAAATTCTTGGCATTCATATCATCATGTTTTTTAATTGATACTAATTCATATTCACCTGGAGTAAACTCACTATAATTAATTAATGAATCTAAAATAAATTTTTTTTCTTTAGAATAAATCAGTTCAATCTTTTTTGTATAAGACTGAGCGCTGTTTTTATTTTTAAAAACAACATCAAAAGTTGCTTTTTCTTCTGCATTCGGTCATTGAAACAAACTTGTAAAATTCATGTATTTACTTTTTTCTTTTTTAAATCCATAAAGCGAACCATCAAGGTAAAAATCCTTGATTGCAAGTTTAGGATTAGCGATACGATATACTGAACGTAAAATACCATTTTGCATATCATAGTCACTAATTTCACTAATTAAATATCATAATTGTTGATAAAAATCTTCTGATATAATTCCTTTTTGTTGCATATTTTGAATATATTGAATAAATTCTAAAAAATATGCATCTTTAGCATCATTGCCTGTTAGTTTTAAATTTGCTAATGAATAATAAGTTAAAATGGTTTTTTGCGATAAAAAATAATCTTTTAAATCTTGAACTCATGTTATTGGAATTTCATTTTGTTTTAAATCAAAAACCGATTTCTCACCAATTTTAAAAAGTGGGGATTTTAAAGTGTTTTCGTTTTTCTCAAAAGGAATTTTAAAGGTTGAAGTGATTTTAATATTTGGAAGTGTGCGTTTTTGTTCAATTTTTGTTTTAAATTCATTAAAAAATGCTTGATCAATTTTCTCAATAATTTTATTGGGTTTTATTTTTGGATTTGGTTGTGATTTTTGCTTACTACAAGCTGTAATAGCAAAAATTGTACATAATGAAGTTAAAGTAATTCCTGCTATTAAAAGCGTTATTTTTTTTGTCTTATGTTTCATAAATTTAGTTCTTTTTTAAATACTTTTCTAAATATTGAGCAGTATAAGATATGTCCTTTTTACTTAGTAATTGCTCTGGAGTACCAGTAGCTATAATTTTACCACCTTTATTACCACCTTCAGGGCCAATATCAATAATATAATCTGCAACCTTAATTAAATCAAGATTGTGTTCTATAACTAAAACAGTGTCTCCTCCATCAACAATTTTATCTAAAATTTTAATTAATTTAGCTACATCATCAATATGCAGTCCTGTTGTTGGTTCATCTAAAACAAATAATGTTTTTCCTGTTGGCTTACGTTGTAAAAACTTAGCTAACTTAATACGTTGTGCTTCGCCCCCTGATAATGAAGTTGAAGAGGCTCCTAACTCTAAATATCCTAAACCAACATCAACTAATAATTGTAGTTTACGATGAATACCAGGATAATTTTCAAAAAATACTAAAGCTTCATCGATTGGCATTTTTAGTACATCATAAATTGATTTACCTTTAAATTTAACTTGAAGTGTTTCATCGTTATAACGTTTTCCATGACATTCTTCACAACTTATATAAACATCTGGTAAAAAGTGCATCTCAATGCGAATCGTACCATCCCCCCAACAACGTTCACAACGGCCTCCTTTAATATTAAAAGAAAAACGACTTTTACTATAGCCACGTGCTTTAGCTTCAATTGTATTTTCAAATACTGTTCTAATATCATCAAATACCCCAACATAAGTAGCTGGATTTGATCTCGTAGTTCTACCAATTGCATCTTGATTAACGACAACGATTTTATCAACATTCGATGCGCCAATTAAATCTTTATACTCGCCTGGAATTACAAAAGGATTAAAATTAGTGTATTCAATAGCTTTAACTAATGTTTCTAAAATTAGTGATGATTTACCAGATCCTGAAACTCCAGTAACACAAATGAATTTACCTAAAGGAATTGTTAAATCAACATTTTTTAAATTATTATGTTTTGCACCTTTTAAAATAACTTTATGACCATTACCACTTCTTCGTGTTTTAGGAACTAAAATATCTTTTTGTTTTGATAAATATTGTCCTGTTAGTGAATTAGGATTTTGCATTACTTCTTGTGGTGTTCCAGCTGCTATTACATAACCACCATTAACTCCAGCTTGAGGACCAATATCTATTAAATAATCAGCTGCTAGCATTGTTTCTTCATCATGTTCAACAACTATAACTGTATTACCTAAATCACGCATTTCTTTTAATGTATTAATTAATTTATCGTTATCGCGTTGATGCAAACCAATTGATGGTTCATCAAGTACATATAAAATACCTGTTAGTTTAGAGCCGATTTGAGTGGCTAAACGAATCCGTTGTGCTTCGCCCCCTGATAAACTTGATGCAGGACGAGCTAAAGTTAAATATTCTAAACCAACATTTTTTAAAAATCCTAAACGATCTAAAACTTCTTTTAAAATTGGATTGGCAATTTTTGTTTGTGCTTCATTCAAATCTAAGTGTAATAAAAAATCAATCGCCTCATCAATGTTTTTTTCAATAAAATCAATAATGTTAATTTTATTAATTAAAACCGATAAAGCTTGTGAAGATAGTTTTTTGCCTTTACATGATTTACAAACTTTTTCACTCATGTATTTATTGTAATGTTCACGGGCTAATTCACTTGATGTCTCTTGATAACGACGATTAACTAATTCTAAAACCCCCTCAACATAATCTATTGATGAATACTTTTTATTATTTGCAGAAGTTATATCAATCTCAATTGCTTCATCTGATCCATATAGTAATAAATCTATTTCTTTACGATCTAATTCTTTTAAAGGTTTAGTTTTATCAATTTTATAGTGTTTAATAATTGAATTAAAACGTTGTCAATCAAGATTAGTTGTATTAACAGTGTTTTTAAAATAATCTAAACCGCCTTCGTTAATTGATAAATTTGGATTAGGGATCATTTTACGCTCATCAGGAACATAATTATATCCTAGCCCTTTACACTGTTCACAAGCACCAATTGGTGAATTAAATGAAAATAATGTTGGTTCAATTTCTTTAATTTTAAAACCACAAACTTGACAAACATCGTTTTGTGTAAAGTAATGAATATCATCATTAACCTTAAATGCAATTTCTCCCCCACTATAATTTAAAGCAAATTCAATAGCATCTAGTGCTCGTGTTTTTGTTTGGTGATCAATATTTAAAATTAAACGATCAACAATGATTGCAACATCTGTTTTTTTGTTATCTAAAAATTGAGGCAAATTTTCATCTAATTGATAAACTTCATTATTAACTAAAACTCTTAAATAACCTTGATTTCTTAAATTATTTAAAACATCATTAATTTTATAACCTTTTTTAATTTCAATTGGTGCAAGAATTTGTATTTTACTTTTAGGTGATAAGCTAAATAAGAAATCAGCAATTTGTTTTGGAGTTTGTGATTTAATTTGACCATGCCCATTTGGACAAAAAGGTGTTCCAACACGAGCAAATAAAACTCGTAAATAATCATAGATTTCTGTAATTGTCCCAACAATTGATCTTGGATTTTGATTTGTTGATTTTTGATCAACAGCAATGGTTGGTGATAAACCTTCAATACTTTCAACATCAGCTTTATCATTTCCACCTAAAAACTGGCGGGCATAACTTGAAAGTGATTCAAAATAACGACGTTTTCCTTCAGCAAAAATCGTATCAAAAGCAAGCGATGATTTACCTGATCCTGAAACACCTGTAATAACAACTAGTTTATTTTTAGGAATTTCTAGATCAATATTTTTTAAATTATTTTCTCTAGCGCCTTTAATGATAATTTTGTCCATATTTATACCTAATAGTTATTGTTTTTAATAATAAAAATTGTATTTTATTTATATCATTTTAAATGATAACAATTTAATATATAATATAAAAAACATATATAATTTAAATACCTTAGGTTATTTATTAAAAATAATCTTCTTTGAATCATAAATATAAGAATGAAATATTTGGTATAAAATAATAAGGTGAAACCTAATAATATTTAATAATTAAGGAGTATATTTATGGCTAAATACATTAAAACAGGTGTTTCTTACATTAATTTAGACAACGCACGTACAATTAATATTCTTCCAGAAGATATTGATTCATATTTAGAACTTGGTGGAGATGAAGCTTATAAAACAAGCGATCTAGGATCAGAGTTGTATATTAATTATGCTGATTTTGAATCAACAAACATTTTATTTGATCTTAAAAAAGAAGAATTACAAGCAAAAATTGATGCTTTTTTAATTTCTAATGATACAGTATTAGATTTATCAGAAGTTTTCTTAGATATTCATTTTAGTGAAGATGATGATTATGAAGAAGATTGCTGTTGTGAAGATGAATGCTGTAGCGATGAAGAAAATGAAGCTTGTTGTAATTCAGAAGTAAAAGTTGAAGAAGAATGCTGTGGCGGAGCTAAAGATGATTGTTGTGGTGGTCACGAACATGAACATGAAGTTTGCTGTGACTCGGAAACAAAAACATCAGAAACCCAAGAAGAATGTTGTGGTGGAACTAAAGATGATTGCTGTGGTGGTCACGAACATGAACACCATCATCACAGTCACCAACATTAATTAAAAAGTATTTAATTAAATGATAGAGATTACTAAGTTAATTAGGAATCTCTTTTTTATTTATATATGGTTTTAAAATTAATAAATCCATATTTTTATATATAATTCTAATTAAAATTCTTGCTAGTCTTTTATTTCTTTCTTGTGTAGTACTTCCTAAACGACTATTATTAACATCAATATCATTAAATAACCCTTTTAAATTTTTTTCAGAATCATAACCAGAGGATGATCCTTCAATATTGTTAAAAACAGTTTCCAAAGTTTCATTTAAATTTTCATTTTTTTCAGCATTTTTAACAATATTAGCAAACAAAAATGACGGCTTAATATAAAAACCTTTTTCTTTAATTAAGGTTATTTTCATTTCTTCATCTACATCATCATCATTACATGTTTCATAGTTAAAATCATGATCACCTATTTTATGCTCATTATCGTTAATATATTTAGTTAAGTTTTCTGATATATATCGATAAAATAATATTCCTAAAACATATTGTTTAAAATCTCATCCATCAATTGCGCCACGGAGCTCATCAGCAATTTTTCAAATTGTTGCATGCAAATTGTTTCGTTCAATTTCTTTTTTATTATCCATATTTATAAACCCTTTATAAATTTATATTTTTAAGTTTGTTAATTAGAATTATCAAACACCTTTAATATTCCACACACATTTGTTATTGAAAATTTGCCTTTTCTATAGAAAACAGTTCCCGCATATGCACCATCTGTAGTTCAAGTAACATATTCACCATCAAAATCATATTTATTAATATTTCCTAAAACACCATCATTTAAACTTTGTGATGAATATACTGGATAATTTCCTTTATTAGAATTAATAAATTCCTTAGAGTATACTTGACCTCTTTTTATTTCACACACATATTCAATCTTTTTAAACTCAATTTTATGATCAATAGATTTTATATCATCACTGTCCATTATTTTCTTTAATAATGTTTGATTTTTATTAAAGTTTAGTAATTCATTGCGATAATATATATACTGCTTATTTCTAAGTTCTAATTCTGTTTCTAATTCTGTTTCTAATTCTGTTTCTAATTCTGTATACTTGTCTAATATTTCAACAATTTTATTTTGGATTGAAATGGGCGGAATAGGGATTGCAAGATTTAAAAATTCTTTTTTATTTAAATTTTTTAATGTACTACCTATAAACAATTTTTTATTAATAAAATTAGAAATATTTAATAGATAGAAGTAAAGATATTTTGTGTTATATTCATTTACTTTGAATGCGAATATATGATCAGAATACATTGTTTTTCCAAAATTATATTTTATATCTGCTTGACCGCCATCATTAACAAATATATATTCTCCATCAACTAAAAAATTGTCAACATAAAATGATTTCTTCCCTGATGTTATTGTTAAATAATTTCCCTTTTCCATTTTTAAAAGTTTTGTAACTCCAAAAGGTGATTTAGGAGCCACTGTAATTATATTTTTTAATTTTTTAAACTCCACTCCATTAGGGCAGAACTCATTAATTAATTTTTCAATTTTATTCATAATTTATTCTTCTAATTCTAAAACAATTTCATCAATTTCTTTTCTTAACTTTTCGATTTTAGCCACAATTTCTTTAATTTGCATATTTAATAATTTAATATCAATTTTTTCTGACGTGTCTTCTTTTTCCACATATGTGTTAACTGATAAATTATAGTTTTTATTTTTAACTTCTTCTCGTGATACAAGTTTTGAAAAATAAGTTACTTCTTTTTTGAAACGAATCGTATCAACTATTTTTTTAATATTAGCATTGGTTAATTTGTTTTTGATACCAGATTTAACAAATTCTTTTGATGCATCAACAAACAAGATACCATTAGCATTATCATTGTTGTTTTTGTTTTTTCTTAAAACTATAATACATGTTGAAATACTTGTTCCAAAAAACAGATTATCTGGTAATTGAATCACACTATCTACAACATTAACATTATCAACTAAATATTTTCTAATATCTTGTTCTGCATGATCTCGATATAAAGTTCCTGGAAACATAACTATAGCTGCTGTTCCACTAGAAGATAAATTATGAAGTATATGCAATACAAAAGCAAAATCAGCTTTTCCTTTAGGTGCTAATTGAGTAACATGAAATCGCTCATCGTTAGCTAATAAAGGATTTGATTTTCCCTCCCATTTTGTTGAATATGGAGGATTTGAAACAATTGCATCAAAAGGTTTATCATCATTATGTTTAGGATCTAGTAATGTATCACCTAATTTGATATCAAATTTATCATAACCTATATTGTGCAAAAACATATTAATTCTTGCTAAATTATACGTAGTAAGATTAATTTCTTGACCATAAAATTTAACTCCTTCATTTAATTTTGCATACTTTAAAAGTAATGATCCAGAACCACAACATGGATCATAAACTTTTCCAATTTTTCTTCTAACATCTTTATTTTCATTATTAAAATCAATTAATGTTAATTCTACTAATAGTTCACTAACTTCTTGAGGGGTGAAAAATTCTCCCCCTGATTTACCTGCATTTGCAGCATACATACTCATTAAGTATTCATAAGCATCACCGAACACATCGATTGTATTATCTTGATAATTACCTAGATCAAGTTCATTAATTCTAATTTAAACACAAAATATTTGAGCTTTATTTTGAGAAAAATTACTAAAAAACATGTAAATCAAGCATCAGGAAATCCAAAATTAATGAGTAATGTTATGCAAGAAATCATAATCCCTATTCCGCCCATTTCAATCCAAAATAAAATTGTTGAGATTTTAGATAAATTGGAAACATATACGAAAGATATTAATACTGGACTTCCTCTTGAAATCGAACAACGTAAAAAACAATACGAATATTATCGTAATAAATTATTAGACTTTGATAATATAGCACGAGAGAGAGAGAGAGAGCTAAGTAGGGATTACATTTGAACATTAAAGAATATATATGAAAAATTAGTTCAAAACAATGTTAAATATAAAAAACTTTGAGAAATAGTTAATTTTGACAAAAAATTTAAAGGAGTTCCAAAAGAAAAACAAAATGAAATATTGTCATTTAAGCATATATCTGCAAACGAATTAAAACGATATGAAAAGTGTAATTTTGGTAATGTTAAACTTCTTTCAACAGGATTGTATGATGGATATATAAAATATAATGAAAATGATAATAATATTAATTATGGCGAAATAATTGCTTTGCCATCTGGTGGTTCTCCAATAATAAAATACTATAATGGTTATTTCATAGATAGTTTAAATATAATTTTTTCACAAAAAAACAAAAAAGAATGTAATTTAAAATTTATTTACTATTTTTTGATAGCAAATAAAATGCTTATTGAAGAAAATTATCGTGGTGCATCTGTAAAACATCCAAACATGATAGAGATTATTGAATTATTAATTCCTATCCCTCACATTTCAATCCAAAATAAAATTGTTGAAATCCTAGATAAATTAGAAGCATATACAAAAGATATTGGCGTAGGATTACCTTTTGAAATCAAACAACGCAAAAAACAATATGAATACTATCGAAATAAATTATTAGATTTTAAAAAATATTAAAAGGAGTTTGTTTTTATATTATGGAAAATAAGGTACTATTAGCAGAACATGAAAATTCAACGGTTGTTGCTGAATTTAAATCAATTAAAGTTCATTCGAATAAATTTGAAAGTGAAGCTGAATTAGAAGAAACATTTATTAAATTATTAATTGCACAAGGTTATGAATACTTACCTATTAAAAATAATAATGATTTAAAAAATAATTTAAGAAAACAAATCGAAAAACTTAATAATTATCAATTTACTAATAATGAATGAGAATCTTTTTTAAGTACTTATTTATTAAATCCTAATGATGGAATTGAAGCAAGATCAAAAAAAATTCATGAAGATTATAGCTACCCTTTAAAAAAAGATAAAGATAGTATCACCAAAAATATAAAGATAATTGATAAAAATAATATTCATAATAATTCTATTCAAGTTATTAATCAATATCAAAATAACAATTTAGATAATCAACAAAAAACACGATATGATGTGTCTATTTTGATTAATGGACTTCCATTAGTACATATTGAACTAAAAAGACGAGGAACTAATCTTAAAGAAGCTTTTAATCAAATTAAAAGATATGCAAATCATTCGTTTACAGATTTATATCTTTACACGCAAATTTATGTTATTTCTAATGGTACACAAACTAAATATTATTCAAATACAACTAGAGAAACTTCTATAAAAGAAAGAAATAATACAAACTCTAAAAAAACAAGTAATTCTTTTGAATTTACATCATACTGAGCTGATTCAAAAAATAATCAAATTCTTGATTTAATCGATTTTTGTCAGACCTTTTTTGCTAAACATACTATTTTAAATATTTTGACAAAATATTGTGTTTTTACAAGCGATAATACGTTAATGGTTTTAAGACCATACCAAATTGTTGCAACTGAAAGAATTATTAATCGAATTAAGATTTCTTTAAATGATAAAAAATTATTAGGAACTACAGGCGCTGGTGGTTATATTTGACACACAACAGGAAGCGGAAAAACTCTTACATCATTTAAAACTGCCGCTTTAGCAAAAGAAATTGAAGGTGTGGATAAGGTTCTATTTGTTGTTGATCGAAAAGATTTAGACTATCAAACAACAAAAGAATATGAAAAGTATTCTAAAGGATGTGTTAGTGCTAATACAAATGTTAATGAACTAGCTAAGCAAATTGGAAATCAAGAGAATAAAATTATTATTACAACCATTCAAAAACTTGGCATATTCATTAGTAAAAATCCAAAACATCCTATTTATTCTAAAAATGTTGTTCTTGTTTTTGATGAATGTCATCGTTCTCAATTTGGTGATTTACATAAAAAAATTACTAAAAAATCTTTTAAAAAATATATGATTTTTGGGTTTACAGGCACACCTATTTTAGAAGAAAATAGTAATGTCAAAAATAAAAATATTTTCTTTACAACAGAGCATCTTTTTGGAGCTTGTTTACATAAATATACAATTGATAAAGCTATTCGTGATGGTAATGTTTTAGGTTTTAGAACGGAGTATTATAAAACACTTAGCGAAAAAGATGACATTGACGATGAAACATCATCAACTGGAATAGATAAAAAAGAAGCTTTGGAAAATCCGATTCGTATTTCTGAAATTGTTGATTTTATTTTAAAAAACTATAATCGTTTAACCAAAAGAAATGAAGAATTTTTTGAACATAATAAGATTGCTAATATAAATGAAGTTATTACTAGCAGAAATAAATTAAGCGAAAATAAAGTATTAACAACACTAAATGGATTTAATTCTATTCTAGCTTGCTCTTCTATTAATGCAGCTAAAATATATTATTCAGAATTTAAAAAACAACAACAAAAATTTCCTCAAGAAGAAAAATTAAAAATTGGATTAATTTATAGTTATTTACCAAGCGAACAAGAAAGTAATGATAATGAACTTTCTAATTTTGAACTATTAGATGAGAATAACGAAAACACTAATCAACTTGATTTAGATTCACGTAATTTTCTTGATGAAGCGATTAAAGATTATAATGAAATGTTTGGGACATCATACTCAACAGATAATGCTAATTTTAATAATTACTATAAAGACTTTAGCTTGAGATTAAAGGATCGAAGCTTAGATATAGCAATCGTTGTTAATATGTTTTTAACAGGTTTTGATGCAACAACTTTAAATACATTATGATTGGACAAAAAACTAAAATATCATAGTTTAATTCAAGCAATGTCGCGAACCAATCGAATTCTTAATGGTGTTAAATCAAATGGTAATATTGTCTTTTTCATAAATTTAGAAGATGAAGTGAATGAAGCTTTAAGATTATTTTCATCAGAGAGCAATATTAATTCTTCGATCGCTATTTTAAGATCATTTAAAGAATATTATGAAGGATATTATAATGAAGATAATCAATATATACAAGGTTATCGAAACTTAGTAGAAGAATTAAAAAGTAACTTTAAAATTCCTTTTAATGGAACAAAAGAAGAGGAAAGAATTTTTATTAAACTTTTTGGTGATATTTTAAAATATAAAAATTTGCTTTCACAATTTAGTGAATTTGAACAAAACGATGATCTTATTGGTACAGAAATGCAAGATTATCAAAGTATATATATCGGGTTGCGTGATAAATATCAAGATAAAAAACGTATGGAAACAGATAGCATTGTTAGTGATCTTGATTTTAAAATTGAATTAATTAGTCAACAAGATATTGGTGTTGATTATATTCTTAAGTTAATGAATGAAAAGCTCAAAAAAGATCTAAAAGAAGTTAAATATCACGATCTAGAAAGAGATATTTTATCTAGTCTAAAAGCGCGAAAAAAGAAAGATTTAATTATTGAATTTGTTAATGAAATTAATAAACTACCTAATAAGGATGAAATTGATATTTTTAATGAATGGAGTAAGTTTATTAAAAATAAAAAAGAAAGTGAGCTTATAAAAATAATTAATGAAAATAAATTAGATAACAAAAAAACATTTGAATTTATGAAAAAAGCATTTGAAAATAATGAATTTAATGGAAATGGAGTAGATATTGTTAATTTAATGCCTCCTACCTCTTTTTTTAAAAAAAGTAATCAGAATAGTAATAGGTCTAAAATAAAAGAAAATGCTACTGAAAAATTACTCGAATATTTTGAAAAATTCCGTGGTTAAATGAACATTAAAATAAAAAATATTTGAAACAATAATAGCGTTACAATTAGTACTTATTTTAATTATATAATTTAGCATATATTTTTAAAGAAATGAGATTATTAATTTAAATAAAATTAAATGTAAATTAGAAAATTGTTTTGGTATAGATAAAATGGAACACACATTTATTTTACCAGATTAAATATAATTACAATTTATGATAAAAATAGATTAATGAAAACTTCATTAGCAAATACTTTCAAAAAAACAAAAAAATAAACAGAGCGAAATAAAAGACCAAATTTTTGTAACACCTATGTATGAAATACCTGTAGATGGTCGCGAAATAAGAAGTGATGAAGTGTTTGTAATTAAACAGTTTTGAAAATTATTATGAATCAAAATATGCTTCAGATTTATTAATACATGAAGATTTAAAACAAGATTTAGAAGAAAAATCTAAATTAAAAATTGAAAAATTTCATTAGAAAAAAGGGTTTGTGAATTAAAAGAAGAATTAAATTCATTTTTTAATAAGGGTGATGATTTATTATTATCAAAATTAAAATCATTAATAAATAATAAAAATAATTCATATATTCCAGAAGTTAAATAGACAAATATTTTTGATTCTTCAATTTTAAAAAAGATAAAGGATATTTATTTTCAAAATCATAAATTTCTTCTAAGGGTTTTTCCCTTTCAAAATTAAAAAATATATTAAAAAGTTTAAAATCTAATAATTTTTTGTTAATGAAAATATAGAAATTAAAAGTATAAAAACTTGAAAATAAAATAAAAGAAATTGAATCAGAATTTAAAAATATTTCAGCTTACAAACAAATAATAAAATCATTAGAAGGTTCTAAGAGTATAGCACTACGAGATGCTATAGAAAATGATCCTTATTGGAGAAAGTTTTCCAAAAATTAAATTTCAATTCAAAAAAATAATGTGTTAAAAATTTTAAATCGCTCGAAATTAAAAAAACAATACTTTTGCGTCAAGGAGAAAAAAGGTCTTTATATTTATTAAATGTAATATTTAATAAAGAAAAATTGAAAAAATAATAAGAAAACTCTTATTGTGATTAATGATATTGCTGATTCTTTTGATTATAAAAATATATATGCAATAAAAATTTCAAATTTAATAGTTTTAACTCATAATTTTGATTTTTATAGAACAATATCATTGGGATTTGATGTTTGCAAAGAATCGCAATTTGTTGTATAAAAAAGAAAGATAAAGAAAACTAATATTATATATTTGCTTTAATTCCTTTTATAAAAAATTTAATAGAATTTAGCTTTAACAAATCGATTTCTAAGGCAGAAAATTTTGAAAATGATCATTAATTTCCAAATGCTAATAGATTTATATAAAAATTATATTGGAAAAGAAAATATTCAATTACCAAGTAATTTTTCAAAAGAAGATATTGTGATGAAAAAAATTCAAAAAGTAGTTAAGTAAAATAACAATGATTCAAACGATTTTGAATACAAAATTTTATTATCAATAGTTTGCAGAATTAAGACTAAAGAAATTATGACGATTAATGATGCTCTTATAATTACACCAAAAAACATTCATATTAATCAATTTATGTATGAACAAAACTAAACAACAAAAGTATAAGAATTAACTAATTTAGTGCGATTAGGATCACTTAAATCTAACATTGGCTTAGTAAAGTGTAATGTGATAACTACTTTTTTTAAAATATTTAGATTGTATTTAATTTCATCATTATTATTTAAGACGTTTGTTTGATCACTATCGTTAGTGTTTAAACCAAAATTAAATGCAACTGCACTCAAATAAACATCTTTTTTTAAATCAACTTTTTTCCCAAATCCAAGCAATTTATAATCAATTAGTTCTTGAAGCGTTAAATTAATTTGATCATAAGCACTAGTTGCAATGATTTTTCGTCCTAATGCGTCTTGATATAATTCTTGACCATCTGTTTTAATAGTTTTAATTTTAGCTAAAATTAAGTTACGAATTGTTTGATTACCAACTTTATTTTCTAAATTATTAGAAAAATGTTTGAAATCAAAGCCTTCACAATATGTATTTGAATCATAGTTTTTTAAATACTTGTTTAAATTATCTTTGATTAAATATTTAACATTTTTGTCATTAGGAATCTTTTTATCACCTTCAAAAGTTTCATAAATCGCTAGTGGATTTATGAATACTCATTTTTTATAAAATAACCAATTTAATAATTGAGCAACATATTTATTAGGTAATAAACGATCGTTTCTTAATGATATATTTTCAATGATTGCTTTCATAGAATTAGGCATATGGGTTTGAACTGTGTGTGTTGGATAAAAAACATAACCAATTTTATATTTATCATGATCACTACGGTCTTTAAAAATTAAAGTTGTTAACTCTTGTGGTTGACTAATGCTAATTGTATGATCAACATAATCGTTTTTTGCTAAAACAATTTTATAAGGAAATTCTTTTTTGATTTGTTCTCATAATCCACCAAGATCAATAAAACTTAATGGTTTTTGTGGTAATAAATCAAATAAAGGTTTAATATCAAATTCTGCATTAGCTAAAACTTTATAACGATATTTAATATTTAGTTTCTTAATTAATAAATTGTTTTGATCATATTCAAGTTCTTTACTTGTGATTTCAGGCTTGCTAATCCCATATTTAAAATATGATTTTAAATCATTAATTGAAGGATCATTAGATGTAATTTTTGGATATAAAATAGCATCTAAAAGTTTTTTAATATTCTCTTTATTTCAATTAGTATTTTGAATTAGTAATTCATCAATAATTTTATAAATAAATTCAGGGATTGCTATTTGAAATTTTTTTAATAAACTAAGAATCATTTCAACACCCTTGTGATAATCAAGTTGATCATTTTTATTTAAAGAAATCAAATCTAAGATTTTAATTTTTGTTAAAAAATAATTGGTACTGATAATTTGTTGGTCTTTAGGGTCATATTGGTTTTCATGCGGAATTAAGTTATTTGCAATTTTTTTAACTTCAGTAATTAAATCATATAAAGTATAATTTTGATCACCAATTTTAAAATTAATACTACTTGGTAGATCATTTTGTTTTAAATTTAAAAAGAAGGAAAATAGTTCGTCCAAACCATCAATAAATCCATAATTTTTAGGGTCTTTTTTAGAACGTGCTTCAATTAAAGAAATTAATTCTAATAATTTATTAATAATTGGTTTATAAGTTTTAATTTCAGCTAGTGAAATACTATCAAGTAAGGTTCTAATTTGTCCAATTTCAGCAATTAAATTATTTTTAAAATCCATTGAATCAATTTTTGATCAAATTGTATTTTTAACTGATTGGTCAATGCTTGGATTCTTATAAATAATTTCTTTTAAAATTGGTTTAAAACCATCGATAACCATCCCTAATGATTGATTTGATGTAATTAAAGTGATTAAATTAGTCAAATTATTCTCTTGTGTAATGTTATAAGTTAAATTTCTAAAATCTTGTGATGATTGTAAAAACAATTCTTTTAAATTTAAATCATTAGCAACACCTTTTAAAATGTTAAAAATACTATCAATGGAATCAATTGCTACATCATAACCACTAGTAAATGCATATTCAATATCTTTTAAAATTTGTTGTTCATTTAATTTTTTTAATTCGTTTTTAATTTTATGATCTAAACTAAAAATATCTTGTTCATATGAAGCTTGTGTGATTTCATTATTTAATGTGTATGAGTGTGTACCAAAGCTAAATTTTTCATTATCATTAGCACCGAAATAAAATTCTTTTGGAAAATTTTCTTCTGCATTTTGGTCTCCACGTTTAGCATTGTTTTTAACCTTTGTAACTTGTTTTTCAACACGAATATTATTAAAATAATAACGCATTTTATATGTGTTTTTAATTGATGTGGTTTGTGCACTATATTTATCGCGTTCTAATCGATCGGTTTGGCTAAAATCTAAAGCAATCGGCGATGCTTCAATCCTAATAAAATATTTTTCACTAGTATTTGAAGCAATCCCAGAAGGTTTTTTATAAAATGGATCATTGATTAAAATATTAGCACTACTTTGATTTGTTAAAGCATACCAAAAACTAATGCTAACTCTTAACGCCTTATTCTTTTCTAAAAGATTAATATTATTTTTAATATCTTGTTCTGTTAGTGGTTCAAAAATGGCTTGATTGGGATTTAAAACTTGTTTTTGATTATTTCCTAAATCTTTTCAAACTTTGATTTGTGGTTCACTTGATGAAAAAACAAGTGATGGTTTAGTGTTGTCTTTACGGATATTATTAATAAAATTATAAAGATCATTTTTTAATGTATTAGGCGTTAATTTTAAAAATCAATTTATAAAATAGGGGTCATTAATATCAATAACTTTATTTAAATTTTTATATTTTGATGCTTCATTAATTCAAATGTATTCATAAAAATTCTTGATTTGTTGATCGTGTTGTTTTTTAACACTATCATATTCTTTTAAATAATCATTTCAAAGAATTGGTTTCTGTTCATAATTATTATTTCTCAAAATTTGTTGATTTAAATTAGCATCATTTTCTGGTGCTTTAATTATGTAGTCATTTACATCAAAACCAGTAATTGCGTCGTTGGTTGTTGATCCATTATAAATAAAACCTGCGTTTACTATTTTATTATTTGTGTTAGCACATGCACTTAATGAAACTACAGCAATACTACTTATTGATAATGAAGTACAGATTATAAATCATTTACGACTTGAACTTCTTTTTTTAAACATAGTATTAATTCCTTAATTTGAATTAATTTAATTGTATTCGTATAATTATAAAGAATTTAAATATCTATTAATATTAAATAATTAAAATTTTCGTTCTTTTTTTAAAGAATGAAAGATATTCTTATTTATTTTTTAAAACACTGTCATAGTAGATTTTCTCTAATGCATCATATTTATGACCACTATATACTAGCTTGCCTTTGTGAATTAAAGTCAAACTATCAACGTAAGTATCCATTTCTTTTAAAATATGCGAACAAATAAAAATGGTTTTGTTTTGTTCGTGCAATTCTTTTAAGATATTATTGAATTCATGACGTGCTGATGGGTCTAAGTTCGCTGTTGGTTCGTCTAAAATAATTAGCTCTGGATCGTGTAATAAAGCTTGAATTAAAATTACTTTCTTTTTTTGTCCAGATGAAAAATTGATTGGTTTTTTATTTTTTAAATCACTAATTCTAAATTTTTCTAAAAAATTATGAATGCGTTCAATAGCTTGTTTTTTATTTAAATTTGATAAACGAGCAAATGAATATAAATATTGAAAAGTTGTTAATTCACTAGGAAATAAAGCAATTTCTGGCACATAACCAATTTTTTCATGACTTTTTGGGGTTTTATTATCAAAACCATCAATTAAAATTGATCCTTTGTAATTTGTGTATGCATTAATAATTGATTTAATAGTTGTTGTTTTACCTGATCCATTTTCACCAATAAATGCATGAATTTCGCCACGTTTTACATTAAAACTTAAATCAAAAACGCCTTGGTTTGATTTTTTATAAATTTTTGTTAAATTTTTAACTTCTAAAATAAATTCATTATTCATTAACGATAGTCCTTTCTTTTATAACCATAATATGTACCAATTAATAAACTAAAAATTAATATTAATCAAATTACGCAGTAAGCTTCTTTATAAACAATTTGTTGCTTTCTTTTAAAACTATAATTTTGTTGGACATAATCGAATAAATAATTTCCACCTTGTTCTAAACTATATCTGTAACGAATCTTTTTATCTTTTTTTAATTCATCTATGCCATCGTCATTTTCTTGAGGTAATATCTGATGAAGTAGGTAGCTTTTAAAACCACCAATTAAATAATTTTTATTGTTTAAATTTATTTTTAATTGTGTTTTATATTGCAAATTATTTTGATGATCAACTAATAAAGTTTTTAGTAAATTTGTATAGTGTGGATTAAAATATAGATAATAAATTAAACTAACTGCTAAATATATTTGTTGCTCAGTTTTTGATTTAATTAATAATTTATTAAAATCCTTTTTAAATAAATTAGCATTCTCATCGTTTCATTTATTGAAATCAAATTTTTGTACACTATTTGAAATTAAGTCTAAGATTTGTTTTTGATTTGCTTTTTGATCAAGTGTTTTAAATAAATTATTAGCATATGTATTAAACGATTTTACGTTTAATACTTCTTTAATAATCATTCATTTAAGCGAACCAACAATATCATCACTGCTTAATTGTGTATAACTGTTTTTTTGATATTCGATCGTTTGATCTTTTCAATTGTCAATTGCATAAATTACTTCTTGATTTAAGTTATTGTTAATTAAATTTTGAGAATCGTTTTTTAACAAACTTGGAATTAAAAAACTTTTATCATTATTAATACTAAAATTGATTAATGAATTACTATTTTTTTCTAACTGATAATTAAATTGTTTTGATGCTAAATTATTGGCATATAAAATCTGATCTACTTGATTTTTAATAAAATCTCTAAATGGCTCATAATCACTTTTATTAAAAACATCAGCTAATTGATAAACAGGAAGAATTCAACTACTTACTTGTCAAAAAGTTGCTAAACTTCTAGTTTTTAAATAAGCGTTCATAATTTCTAAATTTTGATTTTCATTAAATTTATGATTAAAAAAACCTGTGTTAATTAAATAATATTGATCTAAATTATTATTTAAATAAAAAGCTTTAATATTGTTTGTTGATTTTTGTTTTAATAATCTTAAAAAGGCTTGTGATTGAACTTGACTAACTAAGTTCGCAATAATTCCAATAGTAAGCAAGGGGACAAAACTAACAAAAGGAATTAAAATACTTATTTTTGCATTTAATTTTAAAGCTAAAAGGCTTGTTATTAACCCAAAAATTAAATAACAAATAAAAGGACTTATATAACTTAAGATTCAATAATAATTAACATTTTTAGCAAAATTAAAGCTAATTAAATAAAAAATTAATAGTCCTAAAAAACTAACTAGACTTCAAACAATACCTGTTAGGACGAAGAAAAAAATTTTTGATCAAACAATGTTTTTACGGCTAATTGGTTTAGAAATTACTAAAATATCAATACCATCTTCGCTAAAATCCTTAAAAATATTTAATGCTTTTAGTGAAGCAAAAATTGTTGTCATTAATAAATTAAACATGATCATTACATATGAAATTATCAAAAATTGTGTTTTTTGCTTAACAACACAGCCGACAACAATTCCTATAATTAATGTAATTAATAAAGTTATGATCGGTAAAAGATATGTTGTTTTTTTTACAATAATAAGGCGTGTTAAAAAACCAATATAAGCTAAACTGCTGTTCAAATTTATTTTTTTATTCATTTTTATGTGATTTTTCCATATATTAAATATTGTTAATAATTTTATTCAAAAGATAAATTTAATTTTGGTTTTATTGTATTTTTTTATTTTGATTATTTTGTTTTTAAAAATATATGGAATAATAAGAAAAATTATTAAGATAGTGTTTTATTTTTTCTTATTCAACAATTTATTATATTAAATAAAATAAAAAAAATGGTGTATAACCATTTTTAACTAAATAACCCACCAGATTTACTAAAAGGATTTTTTCCTTTTTGTAACATTTTACCAAGTTCTGCCATTTTATCACGTGACACTTCCCACTGTTTTAATAACTTATTTAATTCATCAGGCGAACGTCCTGATCCTTTTAAAACCCGTATGCGTCTTGATGGTTCTTTTTTAAAAACGCGCGGATCACGACGTTCTTTTAAAGTCATACTTGATAATAAAATTGTTCATATTTTCATTTTGCTCTCAGCATCACCAATTTGGTTTTCAGAAATATTGTTCAATCCAGGAATCATCTTAGCTATTCCTGAAAAACTACCTAATTTACTAATTTGACTCATTTGACGCATTAAATCTTCTAAATCCATTTTTCCTGCCATCATTCGTTGCATCGAACCACGAACTTGTTTTTCATCAATAACATCAGCTGCTTTCTCTGCTAAAGTCATGATATCACCAAGACCTAAAATTCGATCAGCCATTCGTTCTGGATAGAAACTATCAATTGATCCTATTTTTTCACCAGTACCTGTGAATTTAATTGGTATATTTAATAATGATGTTAATGATAAAACAGCTCCAGCACGTGCATCTGAATCTAATTTAGTAACAATAATACCTGTTAATTTTAATCAATTATTGAATTCTGTAGCAACGTTAATAATGTCTTGGCCTGCCATAGCATCAACAACTAAAAAAACCTCATCAGGATTTAGTGTTTTTTTAACATTTACTAATTCTTGCATTAACTCTTCGTTTGTTTGTAAACGTCCAGCTGTATCAACAATTATCAAATTATTTTCATTTTCATCAGCTTTGTTTAAAGCATTTTTTACAATTAAATCAGGGCGTTGTGTTCCTTCTTCTCAAAAATCTACACGGACTTGTTTTGCTAATGTTCTTAATTGATCTATCGCTGCTGGGCGATAGATATCAGCCGCCACTAATAATGGTTTTTTATTGTATTTATTTTTAAAATAATTAGCTAATTTACCAGTAGTTGTTGTTTTACCCGAACCTTGTAAACCAACCATCATAATTTTTAATTGTGATTTAGCTGTATTAACTGGTTTATTTTCTTTTCCTAAAATTTCAACCAATTCATCTTTAATTATTTTTAAAACAAGATCTGCTGGCTTTTGGTTTGCTTCAACATACAACCCAACTGTTTTCTCTTTGATGTTTTTAATAAATTTTTTAACAACTAATAAATTAACGTCAGCATCTAATAATGCAATTCTTATTTCACTTAATAATTCTTTTATATCTTCTTCAGCAATTGTCGCATTTTTCAGTTTTTTTGACATTTGCTTACTTACAATACTACCTATCATCGCTTTGAACATTAATTACCTCTTTATATTTTAAAAATTATTATACTAATCTTTAATTATATTATTTAATCTTTATATTATTTATAAAGAAGATTAAAAACACCCAATAAGTTAAAATAAAATAATAAATCTACAAATAAAAAATTCTTAAATTAGAAAATATTAATCCTAAAATTAACTGTTTTGAACAAATTGAAAAACTAGTCAATGAATTTAGTGATAGTTGATTAAATTAAATATAGATAGAAATGATTATAATGTTTTATATAAATTATTAATGATTTCAATCAAAAATTAAGGAATTAGAGTAAAAAATTTAAAATAAATAATTGAAGATTGTTTTTTTAAAAAAATTAAATTTTTATTTTTCGTTTATTGATAAGAGATTGTTTTAAAGTTTCATCATCGGCATAATCAATGCTTGAATTTAAAGGAATACCAAAACCAATACGATAAATTTGTAGGTCTTTAATGTCTTCAAGCATTTTTATAATATAATCAGCTGTGACTTCTCCATCATGAGTATAACTTGTAGCAATAATTATTTCTTTAAAACTACCATTTAAAACACGCTCTTTAATAGTCTTAATGTTTGTATGTTCAATAACAACGTTTTTCCTTAAAGAGATTTCACCATTTGTAATATGATATAGGCCATTATAAATGTTAGCTGATTCGATTCTTTCTAAATCCTCGTTTGTGGTAACAATCATTAATTTAGTAAAATCACGATATTCATTTAAACAAATATCGCATTCGTCTTTATTTGTAAAATTAGCACAATACTTACATTTTAAAATATTTGTTTTTGCTTCTTTAATTCTTTTAATAAGATCATCAATGTATTTTTGATCTTGTTGTAATAAAAAAAATGCTCATTTATTAGCATTTTTTGTTCCTACACCAGGTAATGATTTAAGTGCATCAACAAGCATTTCAAATTCTACTGGTTTACTCATAATTAATTCTAAAAAAGACCTGTTCCTTTAGGGATTGTAGCATTCATAATCGCATCTCGTTCTTTACATGTTGTAGAGATTGCTTCATTGATCGCTGCACGTAAAATATCTTGTAATGTTTCTTTGTCTTCAGGATCCACTATTTCTGTTTTTATTTCAATTGCTTCAATATTTAAATCACCTTTGATTTTAACAAGTACATATCCTTTATAATCAAAATCAAACACTTTTTCTTCAAATTCTTTTTGTTTTTTACTTAAAGTGTTTTGCATTTTTTTTAATTCTTGTGCAAGTTTTTGAAAGTCCATTTAAATTTTACTCCTCATCTTCAATTTCATTTAATAACGCTGTTTGTTCTTGTTTTCGTTGTTCTTCTTGTTTTAATAACATTTTAATATCATTAATTAAAACATCATTAATTATATTAGTTTTTAAACCTTGTTTTTTAATATTAATAATATTTAATGCTTGTTTTTTGCTAATAGCAATAACTAACTTGATAGAATCAAATTTCTTAACAAATTCTTTAATAAATTCAAATGAATGCGTAACTTTATTGATACGATTAATTAATCCTAAAAACTCTACTCCTAAAACTAGTGCATTAGGTGAACATCAAAGTACTAAATCAGCTTGTTTAATAATATCAATATAACTATTTTCAATGGTTTCTTCATTTTTAAAATCTTTAAATTTTTGACTCATTTCTTTTGAAAGTTTAATGTCTTTATTAAAAAAAGCTTGTTTGGCTAGTTCATAATTATCTAATATATCATTAGAATTGCAAATCAATTTATTATCATTAACTAATTTTGTACCTAATTCTTCTTTTTGATTAATACCTAATTCTTTATCATTCTGGTTATTAATTTTTTTGTGATAAAAAATTTTTGTTTTGTATAACTTATCAAAATCAACAGTATTTTTATTCATTTCTTCAAAACAATCGATTTTTTTTGTCGTTATTATTTGGGAATTATTAATTTTATTTTCTTCAGGGTTAATTGACAATTCTATTTTTTTATTTTCTAATTTATCATTATTAGCATTGATTATTTTATATAAATAAATTTCAAATTCAAAAACTTGATTTAATGATTTTTTTAAATTATTAAATAAAGAAATAAAATTATTTAACAAATAATTACCATTAAAATTAATTAATAACATTTTTTTTAATTCATCAACTGATACTTTTTTAAGATTATTTATATCGTTTGTTTTTAAATAAATTAAATAATCAATTAAAACATTTATTATTTGCACACAAAAAGCTTCTAAATTAACACCTTGATTGTATAATTCATAAATAAATTCACGCAATAATAAAATGTCGTTTTTTTGAATTAATTCGATTAAAGAAATTACTTTAAAGTTGTCAACTAAACCAAAAGTTTTATTTATTAAATCAAGATCTATATTTTTTTTAACCGTTGTTAATTGATCTAAAATGCTTAAACAATCACGCCCAGATCCATCAGCTAATTGAATTAATTTATCAATTGCATTATTATCAATTTTGATATCTTCTTTTGTAAGGATAGAGTCTATTAATTCTTGTAATTCACTGTTATTTAAACGCTTAAAATCATAACGTTGGCAACGACTAATAATTGTTAAGGGAATTTTTTGGTACTCCGTTGTTGCAAAAATAAAAATAACGTGTTGTGGTGGTTCTTCAACTGTTTTTAATAAAGCATTTCATGCTGATGTAGATAGCATATGTGCTTCATCAATAATATAGACTTTATACTTAAAAAATGTTGGTAAATAATTTATATTATCAGTAATAATACGTATTTGATCAACACCATTATTACTAGCAGCATCTAATTCAATCAAATCAACACTTTGTTCTTTGAGAATACTTTCACAAACACTACATTTGCCGCAACAATCACCTTTATTAGGATTTAAACAATTAATACTAGACGCAAAAATTCTAGCTATTGATGTTTTTCCAATTCCTCGTGGTCCACTAAAAATATAAGCATGTGTAATTTTGTTATTTAAACAGGCATTCATTAATGCTTGTTTAATTAAATCTTGACCTATTACATTTTTAAATTTATGTGGCCGATATTTACGGTACAATGTTGATTCGCTCATTTATTATCCCCTAAAAACTACTAGTTTTAATTATAAATTAGAATTAGCACAAAATTTAATTTTTATCTATTATAAATACCATAAAATTAACATTTTTTAATAATAAAAAAATTATTTTTTTAATTTTATTTTTTAGTTTTTTTATTTGTATAATCTAAAATATGAACTAAAAAAATATTTATGATAAGGGTTAAAATATGTCAGATAAAAAATTAGATTTTTTAACACCATCTTTTAAAAAAAATAGGGTTTCTAATTCTATTGCAATAGGTGGTATGATTGCTTTTGGTAAATCTACTTTAGCACAAGCTTTACACGAGAAATATCAACCATCAAATGTGGTTTATGAAATGGTTGAAGGTGATAAATTAATGGATTTATTACTAGCAAAAATGTATGAACGTGAAAATAATGTTCTTTTTGGGTCTTTATTTCAACTATATTTTGTTTTAAATCGTTTTGCAAACTACAAAAATAATTGTAACCGTAAAAGTTTAACAATTTTTGATCGTAGTATTTTTGAAGATTGATTATTTGCGCATGCAAATATTGATAAACCTTCTGTATTTTCATATTATGATGGATTGTGACAAGGAGTTTGCAAAGAATTAATCTATGAACATGGAGTTCCAAAACTTTACGTCATTTTAGATGGTGATTGAGAGTTATTCAAAGAAAGAATTTTTAAAAGAAACCGGAAAGTTGAAATTGATAATTTTTCAATTAATGAAACATACTTCAAACGTTTATTAGAAATGTACAAAAATTATATGGTTAATGTTTGTAAAGATTTTGGTATTAACTATGTTGTTTTAGATGCAAACAATTCTGTTGAACAAAACGTTGATGAAGTTACAAAAATATTACAAAAGTTAGAAAATGAGCAAGACAAATAATAAATCAAAAATTTGATTGATCGTTGGTATTATTTTTGCTATTAGTTTGTCGTTTGGTATTTGAGGAATTTCAAAAGCATTACGCAACGACGAGCAAACAAAACAAAAATATCTAAATAAAAATTCAGATAAAAAACATATAAAAGAAAATAATAAAAAAGAAACAAGGTAGTCTTGCTTCTTTTTTATTATTTCATACTATCCAATAATATTTGTGTCAATTGAAATCCTTTTTTCTTTAATTAAAACGATCTTAATTTTATAATTTGTTAATAAATCATTTTCTTCAATTGCAACTTTGATCTCGTGACCTAAGAGTTCTAATTGCTCATCACTTACCAAGTTAGAATCAACAATTACACGTAATTGACGACCAGATTTAATGACATAGGCGCTTTTAACACCTTCGAATCGCATACATATTGTTTCAAGTTCTTTTACACGACTGTAATATTCATCATAAGAATTAACACGTGCTCCTGGTCTGGCCGCTGATAGTGTATCAACTATTTTAACTAATGCACCATAAATTGTTGTTGGAATTACTTTGTTATGATGTGATTCAATTGCATTATATATATAATCATCTAAATTAAATTTCTTAGCGATCAATAATCCCGCTTCTACATGATCTAGGTCGCTTTCAAAATCAATCGTTTTTCCTATATCATGAAAAAATGCAGCTTTTTTTGCTTTTATAGGATCAATATTAATTAATTTAGCAATTCGTTCAGCATATTCAGCAACTTCTAATGAATGGGATAAAATATTTTGACCATAAGAATTACGAAATTTCATTCTCCCTATATAAGAATAAATTCCTTCAGGTAAATCAAAAAAACCTAATGTTTGTTCTACGACATTTTTACCAATCTCAAAAACTTTTTTTTCAAACTTTTCACGTTCTTCTTTAAAAAGTAATTCAATACGATTAATATCAATATTTTTTGAATCAATTAATTTTTGCATAACAATTCGTGCTATTTCACGACGAATTGGATTTGGAGTAGATATTCCTACAATTGGCTGTTGTCGATCAACAATTAGATCAACTCCGCCTTCATTTTCAAAAACTGCTTTATTACGTCCATCACGTCCAATAATTTTTCCTTTTAGATTTTCATCAATAATGTCAATATTAAATAAAGATCTTTCAACAATTAAAGGCTCAGCAATTTGCTCCATTGTTTCTACTAGAATGTTTTGAGCATAAATTTCTTGGGCTTCATTAAATTCGTGTTTAATTTTTGCATATTCTTGAGCAAGCTCTTTTTTAAATTTTATTTTTAATAAATTAAAAAGTTCTTTTTTCGCTTGTTCAGCTGTTAAACTTGTGGCATTTAGTAAAAAATTTTCATTGTTTTTTTTGAGTAAATCAATTTGTAAATTTTGATCACTAATTGCTTTTTTTAATTCATTATTTTTATTATTTAAATCTTCACATTCTTCTTCTAAATCTTTAGTTTTTTGAACTATTTTTTTAAAAGAAGTTTGGCTTTTTGGAACAAAAGTTAATTTCAGTTGTTTTTTCTTAAGATAAACTACCATTACAACTGGAACAATGGTAATCAGCAATACAAATAATATTAGTAATATAATAAATGCTATTAAATAACCCATCTTATATCACCAATAATAATTATATTACAAGTTATTGTCAATTGCTTTTTTCATTATTGACAAACTCCAAAGTTTTTTCTGAAATTTGTATAAAAAGTTCATTGTTTTTTGATAGTGTATTTTTTAATTGTTCTCGCCCTTGCCCTATTTTATTATCATTAAAATAAAATCAAGAACCGTTTTTTTTCAAGACGCCATATTGGATTGCAAAATCTATAATTTCATTATTATAATCGAAACCAGAACCAAAAAAAATATCAATAAAACATGTTTGTAACGGTGGTGCTAATTTGTTTTTAACTGTTGTTACTTTTGTTTTTATACCAATTTTATTAATACCCTCTTTAATTAAATCTGCTTTTTTGGTTTCTATTCTCAAACTAGCATAAAATTTTAAAGCTTTACCGCCTGTGGTCATTTCAGGATTGCCAAAAAAAGTATTAATTTTTTCACGTAATTGATTAATAAAAATAATGGCTGTTTTACTTTTATTAATTAATGGTTGAATTTTACGTAAACCTTTTGACATTAAGCGAGCGTGTGCTCCCATCAATGCTTCATTCATATTTGCTTGATATTCGCTTTCTGGGAGCATTGCAGCAACTGAATCAATAACTATTAAATCTATTGAATTTGTTTTAATCAATGTTTCAATCATATCAAAAGCCTGTTCACCTGTTTGAGGTTGCGTAATGATTAATTTTGTTAAATCGACTTTTAGACTTTTGGCATAATTAATATCAAATGAGCCCTCAAGATCTAACAAAACTACTGTCCCACCAGTTTTTTGACATTCAGCAATTGTTTGTAAAGCAATTGTAGTTTTACCTGATGATTCATTACCATAAATTTCTGTAATTTTTCCTACTGGAATTCCATTAATTCCTGTTATTTGATCTATATGAATTGATCCTGTAGATATTGCATTAATTTTTTCATCTTTTATTTCATCAGCAATAAAATAAGATGATTTAGCAAATTTTGTTTCAAGTGTTGCAATTGGATCTAATTTCTCAAGATTATTATTTTCTTTCATAAATTTATTCACCTATCCTATTTAAAGAATTAGTAAAATTATTTTTTTATCAATTCAATTAAATAACTAATTGCTTCTCATGCTATAGAAAAACGATTAAAAGTACGTTCATTTGAATTTAATGTGACTTTTTTTACAATTGCAAAATCTTTAATTGCTATTCCAATGTAAAATAAACCTACTTCTTTATTTTCGCTTCCTTGTGGTCCAGCATTTCCTGTAATTGAAATTGCAATATCAGTATTAAACTTTTGATTTGTTTGTAAACACATTTCTTTAGCAGTTTGTTCAGAAACAGCGCCATATTTAAAAATTGTTTTTTCACTCACTCCTACTAATTTAATTTTTACTTCATTACTATAACTAATAAAACCACCTGCAAAAACACTTGAAGCACCTGCAACTTCACCAATTGATGATGATAAAGCTCCACAACTTGCTGATTCGCAAACACTCAATCTTAAACGTCTTTCTTTTAGTAAATTAATTAGTTCTAAAGCCACATTCATTATTTTAAAATCCTTTCAAATTGAATAATTATTAGTTTAGATAATAAAAACCACTTTTAGAAAAAGTAAAGTATAACTTTATTTTTTAAAGTAGTTTTTATTGTCATTTATTTTAGATTGTAATCATTTTCTTGTAATAATTGTTCATCATTAATTTCTTCTTTTTTAAAGATAGCAATTGATTTTACTTCTTCTTTATTTTCTAATGAAATTAATTTAACTCCTGATGTATTTCTTCCAATTACACTAATGTCTTTAATCGATAAACGAATAACTTTTCCACTAGTTGTAATAATTAAAGCTTCTTCTTCACGATTAACAACTTTAATACTTACTAAGTTACCTGTTTTATCATTTACTTTTAGTGTATTAACACCTTTTGCTCCACGCTTAGTTAAACGATAATCATTGATGTCAGTTAATTTACCTAATCCTTTAGAACCAACAGCTAAAACATATTCTCCATGACTAGATAACCCTGTTCCTACAACATATTCATCATTTTCTAAATTAATTCCTTTAACACCTGTCGCAATTCTTCCCATTTCACGTGCATCATTTTCATTGAAACGAATTACATTACCATTTGATGCGCCGATAAATACTTCATCATTACCTAGTGTTTGCATTACTGAAAATAATTTATCACCATCTTTTAAAGTAATAGCAATTTTACCATTAGCACGAATTGACTCGAAATCACTTAGATTAGCTCGTTTTATAATTCCTTTACTTGTTGTAAAGAATAATGAACCTTGATCATAATTATCTATTGGTAAAATAGTTAAAATGCTTTCACTTTTTTCAATTGGTAAAAAATTAATTGCTGGAATTCCTTTTGATTGTCGTGATCCCAATGGTACTTCATGTCCTCGTAATCGATAAACACGACCTAAATCAGAAAAGAATAATAAATCTGTATGGGTATTAGCAACTAAGATTTTTTCAACATCATCATCCTCATACGTTTTTAATCCTTGTACACCTACACCACCACGTCGTTGATTTTTATATGTATCAATTGGCAAACGTTTAAAATAACCACGTTTTGACATTGTTACAACAATATCTTCGACTGGTATTAAATCTTCATCATCAATATGCGAACTAACTCCATACATAATTTCTGTACGACGCTCATCACCAAATTTTTCAATTAAATAATCTAAATTTTCACAAATAATATTAACTTGACGATTGTAGTTGTTTAAAACATCTTCTAAATCTTTAATAACTAATATTAAATCTTCATATTCTTTTTTTAATTTATCAATATTTAGTCCTGTTAAAGCGCGTAAACGCATTTCTAAAATTGCTTTCGTTTGCAATTCTGTTAAATCGAAACGTTGAATTAGTGATTTTGAAGCAATATCATCATCACCTGAGGCTTTAATAATTTTAATTACTTCATCGATATTATTAACAGCAATTACTAAACCTTCAACAATATGAATTCGTTCTTTTGCTTTTTTTAAATCAAAATTTGTTCTTCTTACTAAAATCTCAATTTGGTGTTCAAAATAGTATTTAATCATTTCCTTAATGTTTAATACCATTGGTACACCATTAACTAAAGCTAAATTATTAACACTAAAATTTGTTTGTAAAGGTGTTGTTTTAAATAATTTGTTTAATAAAACTTCAGGTATAACATCACGTTTAACTTCAATAACAATTCGAATTCCATCACGACTTGACTCGTCACGTAAATCAGAAATTCCTTCGATTTGTTTGTTTGTTACTAATTCAACAATTTTTTCAATTAAATTAGCTTTATTAACCATATAAGGAATTTCTGTCACAACAATTGCTACACGTCCTTGCCCAATATCTTCAATTTCATATTTTGAACGAATTGTTACTGAACCACGACCTGTATTAAAATATTTAATAATACCGCTATCACCATAAATTTCAGCGCCTGTTGGAAAATCAGGACCTTTTAGATATTCCATAATTTCAGAAATAGTAACATTATGGTTTTTAGCATAAGCTTTTATTGCTTGACAAACTTCAGTTAAATTATGTGGTGGAATATTTGTTGCCATCCCAACAGCTATTCCTGTAGTTCCATTTGTTAATAAATTTGGAAAACCTGATGGTAAAACACTTGGTTCTTGTTCTGATGCATCATAATTAGGTACAAAATCTACTGTTTCTTTATCAATGTATTTTAATAATTCATAAGAGATTTTTGATAATCGTGCTTCTGTATAACGCATCGCAGCTGCACTATCACCATCAATCGAACCAAAATTACCATGACCATCAACTAATAAATAACGCATTGAAAAGTCTTGTGCCATTCTTACAATAGTTTGATAAACAGCTTGATCTCCATGTGGATGATATTTTCCAATTACATCCCCAACTCAACGTGCAGATTTTTTATAAGGCTTATCATGTGTCATTCCGTTTTCGAAAGCAGCAAATAAAGCTCGGCGATGCACGGGCTTAAATCCATCACGCACATCAGGCAAAGCACGTGCAACGATTACAGACATTGAATAATCTAAAAATGATGTTTCGACTTCTTTTGTAATTGATTGTTCTTTAATCGTTGAACCTTCTAATTGTTGCTTAATTTCTTCGGTGGTTAAACGACTTTTTTTTGGTTTTTTTAATGCCATAATATTTTATATACCTCAATTTTAAGCATCAATATTTTTTACATATTTTGCATTTTTTTCAATAAATTCTTTTCTTGGAGCTACATCATCACCCATTAATAGAGAAAATGTTTTATCAGCAATTGCAGCGTCTTCAATATTAACTTTTAATAAAAGTCTATTTTTAGGATCCATTGTTGTTTCTCACAATTGATCAGGATTCATTTCACCTAACCCTTTATAGCGTTGAATGTTATATTTAATATTTGATATTGTTGATTTAATTCTTTCTAACTCTTGATCAGAATATGCGTATTTAATTGTTTTAGCATAACTTACTTTATACAATGGTGGTTGCGCTATATAAACATTTCCATTTTGAATCAATGGCAACATGTATCGATAAAAAAATGTTAGCAATAGAATTCGAATATGACTGCCATCAACATCCGCATCAGTCATAATAATAATTTTTGAATATCTTAATTTTTCAATATTAAATTCAGGACCTATTCCTGCTCCAAAAGCAGTAATCATATTTTGAATTTCTTCATTAGCAAAAATCTTATCAATTTTAGCTTTTTCAACATTAATAATCTTTCCACGCAGTGGTAAAATTGCTTGAAATTCACGCTCACGACCTGTTTTTGCTGAACCACCCGCTGAATCTCCTTCGACAATATAAATTTCAGTAACACTTGAATCACGATTTGAGCAGTCAGCTAATTTACCAGGTAATGAATTTGATTCAAAAGGTGATTTACGCCGTGTAGCTTCACGTGCTTCATGTGAACGTCGTCGTGCTTCAGCAGCTTGCATAACCTTAGCAACAATTTTTTTTGATTCTTCAGGATTTTCATTTAAAAATTTTTCAAAAATAATAGATGTGATTTCATTAACATAAGGACGAACTTCACTATTACCTAATTTCTTTTTTGTTTGACCTTCATATTGAGGATTAGGATGCTTAACTGAAATAATTGCTGTTAATCCTTCACTAACGTCTTCTTTAGTGATTTTATCATCAGTGTCTTTTAAATATTTTTTATCGACCGCATATTTATTTAATAAACGCGTAATCGCTAATTTAAAACCTTCTTCGTGTGTTCCACCTTCAGTGGTATTGATGTTATTACAAAATGAATGGGTTGAGTTATTGTATGAATTATTATATTGAAATGCAACTTCACATTTAATGTTATAAGTTTGCTCGTTATGTCCTGGAACTCTAACTTCTTTTTCTTCTTCTCCATAAACAATTAAATCAAATAATGGTTCTTTTTCAGCATTTAAATCTGTTATATATTCTTTTAAACCACCTTCATAATGTCATTTTTCTTTACGGTTAGTTAATTCTGATTCAAATTCAATGTTAACACCTTTGTTAAGGTAAGCTAATTGCTTTAAACGAGCCATTATTTTAAGCTCATCTCACTCATTTTCTTCCATAATTTCAAAATCTGGAATAAATTCAATTGTTGTTCCTTTTTCTTTAACATCTTTATTATTAATCGGTTTTAGTGGCTCAATAGCATGACCGCCATTGATAAATTCAACATAATATTGAACATGATTCTTGTTTACTCAAACCTTAAAATTTTTACTTAAAGCATTAACAACAGAGGCACCAACACCATGTAATCCACCAGACACTTTATAACTATCATTATCAAACTTACCACCAGCATGTAAAACTGTTAATACTGTTTCAACACCTGATAAGCCTGTTTTTTCGTGAATTTCAATTGGAATTCCGCGTCCATCATCTTCTACACGTACTGATCCGTCTTTTTTTAGAATAACTCTAACAATTGTTGCAAATCCGCCCATAGCCTCATCAATTGAATTATCAACAATTTCTCAAATCATATGGTGTAGACCCTCTGATTGGGTTGAACCAATGTACATACCAGGACGTTTTCGTACTGCTTCTAAACCTTCTAGTACTTTAATACTTTCGGCGGTGTATTTTTTTTCTTTATTAGAATCGTTCATAAAACTCCTATCTACTCATTAGTTTTAACTATTTGATACAATTGATTTTTTATTTTAATAATCATTTGATCATATAATTTACGACCACGTCTATTTTCTATTTTTTCATCAACCATAACTTTATTTTCCAACAATCAAAATTTAGCTTGACCACCATTATTAATCAATCCAGCTATTTTTAAAAATTGGTTTAAAGTCACATACTCTGTTGATATAAAAATTTTTTTCATAGGTCTTAAAATATTTGTAGTAATACATATTTATTATAACATATATAAAATATTAAAAATTTAGTTAAACAACTAGTAAATGTAAGTGATATTTATTAGAAAAATATTAATATTTAAATAATAATAAACTGTTTTCTTTAATTTTTTATTCTTGATAAAATCTGTTTAAATCCAACATCTTTGGTATCGATAAAAATAATAGGTCTTAAAATTTCATCTTTAACTTTAAAAGTTACATCGTTGTTATCAAAATTTTTTAAAACATGAGCTAGCAAACTAGGATTTAAAATAAACTCAGCACTTTTATTGTTTAAATTAACTATTGACATTTTTTCTTTCATATTACCAATTTCAAAAGTTGTTAAACTGATTTCTGCTTCATTTTTGTCTATTCTAATACTAATTTTAGGGTTTTGTTCTTGCATAACAATATTCATACCTCGTTCAATAATTTCTATCAATAAACGACGATCAAAAACATATGTATTTTCTTCATTTGTTTCATCAAAAATTGAGTAAATATTTGGATAAACACCATCAATCATTCTTGTTTGTAAAATTGCATCATTAATTTTAAAAATTAAACTTCTACTTCGAACATAAAAATCAACAGTTTGATTATTACTATATTCAACATTTTTAAGGATTTCGATGATCAATTTCATTGTTGAATAAGAAACAATAAATTTAAATTTTTCACCCATATATTCTCTTATTAAACATGAAGCTTTAATTTTATCTGTACCAATAGCAATCAACTTATTTTCTAAAGTTTGAGTATCAATCAAAATTCCAGAAATCGGTTGAATTTTTTCAATTCCTTGACTGTTCAAAACGTTGGGTAAAACCTTTGTATTAATCTCTTGCATAATTTGATGCGGCAAACTGATTTTAACGTAATCTTCTAAACTAAAGTTTAAATTAGGAAAGCCTTCAATATTCATTGTATTAATTTGAGTTTCAAAATTTTCAGTTTTAATTAATAAAATATTTGTGTCAATTTGTTTTAATAAAACAGATTTATCTTTAAGCTTAGAAATTAATCCTAAAATAATCTTAGCTGTAACTAAAACTTTTCCACTATTAAAATATTCAAATCCTTCAGTAATTTCTTTATAACCACTCACTTGATTATTTGTCGTCTTAAAACTAATTTTATTTTGACTAACTTCAATCAAAACACCTAATAATAAAGCATTTGCATTGTTTGTATTAGCAATTGTTGTACTAAATTTTATTGCTTCAATTAATTTTTTTATAGAAATAAAAACTTCCATGTTTTATTAAAATCCTCATTTTTTATTATTTATATTATAAAAGATATAAATTCAAAAAAGCGTGATGCTTTAAAATATAATTTATTTTTTATTTATTTATTTTTAATTATTAATATTATTATTAAGTGTTTAAATGTGCATAACTTAAAATTCATCTATTATATAATAATTTTATTGAAGAGATTTATAAACATTGTACGTTTATAATATAGGTATTAAATGTGGAAAAATATTTAATTGTTGGATTAGGTAATCCAGGATCAAACTATGCAAAAACAAGACATAATGTTGGCTTTATGGTTATTAATGAGATTTGCAACAAACTTAATTTGTCTCTAGATAGTTCAAAATTTAATGGAATTTTTACTAAAACAATTTATAACAATTCCATAGTGTTTTTTTGTCAACCAACAACTTACATGAATTTAAGTGGTGAATTTGTTATCAAAATGCTTAATTTTTATAATATTCCAATTAAAAATTTAATAGTTATTTATGATGATGTTGATACGAAATTAGGAACGATAAAATTGAGAAAAAAGGGGTCCTCAGGAGGGCAAAATGGAATTAAAAATATTATTAACATTTTAAAAACAGATGAAATTAAACGCATTCGTATAGGAATTGATAAAGATCCACATATAAAATTAGATCAATATGTCTTATCGAATTTTAAGATTGACGAATTAGTTATAATTAAACCAGCTATTATAAAGGGCGCTTTAGCTACATTAGCAGCGATTGGTGAAGATTTTGATAAAGTTATGAACAAATTTAATTAATAGAATTAGTAATAAAAAATACTTAGCTGCTGTTTCTGGTGGTCCAGATTCTATGGCAATGTTAAATATGTACAAAAGAAATATTCGTGTTGTTTGTCATGTGAATTATCATAAACGCGAATCATCCGATCGTGATCAGCAAATAGTTGTCGATTTTTGTAAAAAAAATAATTTAATAATTGAAATTTTAGATGTTGATGAAAAAGTTTATGAACGTTATGGTCATATTGATAATTTTCAAACAAAAGCACGTTTAATTCGTTATGATTTTTTCAAAGAAATTGGTAAAAAATATAATATTGAAAATTTGTATATAGCGCATAATTTTGATGATTTTTTAGAAACGGCATATATGCAACGAGCTCGCCAATCAAAAGCCTTATTTTATGGCATTAAGGAAAGTAATGTTATTAATGGCATAATTGTTAAACGTCCGGTTTTATTTGTGCGTAAACAAACATTACAACGCTATTGTGATGAAAATAAAATTAAGTATGGTATTGACGAAACAAATGAATTAGATATATACGAACGCAATCGTGTTCGTAAAATAATTAGTTCTTGAAGTTTAAATGAGGTTTATGATTTTAAAAAATTCGTTATTAAATATAATAAAGAACATAGTTCTTTTGCTAATTTTATAGATTTATCATATATTGAATTTAAAAAAAATAAATACAAATATGACTACTTTATTCGTCAAGACGATATGGTTCAATATTATTTAATTTACTACTTTTTAATTGATCATAAAATTATTAATCCAAGTGAAAATAAAATCATTTCATTAATCAAATTCTTTGGTAAACAAATTAATAAAGAAAAAGCTTATCGTGTTCAAGAAAATATTTATATGCATGTTAATGATGATGAATTAATTAGTTTAATATCATGCAATAAAAGCGATAATATTAGCAACTCTAATGTTGATATTATCGATGATCATAATGTTATTGAGAAATAGGTAGATAATAATTAATGGAAATTCGTGGTAAATTATTTGTTAGTCAAGTTGTTAGAAAGTTTAATTTAAACGTTGTTGCAAATTCTGACTATATTGATCGTGAGATTTCTACAACAGGAATTACACGTGTTGGATTTGAATTAGCTGGCGAAATTTTGTTTAAAGAGATTTGAAATATTGTTTATTTTGGTTCAAAAGAAAGCAATTATTTTAATAAATTTTCAGAAACGATCATTTCTAAAAAATTAGGAAAAATTTTAGATTTAAATCCACCATTAATTATTTTTGGTAAAAATTTTAAACATGCTGGAATTTTGTTAAAATTAGCAGAGCGCTATAAAATTCCAATTGTAGAGGTTAAATATAGTTTTTATGAATTAAATTTTACAATTAATAGTTACATTAGTCAAAAACTTTCACACCAATCATTAGTTCACGGAACATTATTATCTGTTTTTGGAATCGGCGTTATTTTAATGGGGGAATCAGGTGTTGGTAAATCTGAGTTAGCAATTGAATTAGTTAAAAAAGGTCATATTTTTGTAGGTGATGATGCTATTTTAGTTAATAGAATTGGAGGTAATCTATATGGAAGAGCTGAAGATTCGACTAAAGATTTTATTGAAATTAGAGGTTTAGGTATTATGAATTTTTCACGTTCCTTCGGTATTGAACGAATGATTGAATCAACAAAAATTGAAATAGTTATTGAATTAATAAAACCAGCTAAACATGAAAAAATCAAGTTTGAACGTTTTGGTCGTGAAATTCAACACAAAGAATTTTTAGAAACAAAAATAGCCCATTATTATATTCCGGTTATCGAAGGAAGAAGTATTTCAGATATCATTGAGACAGCCATTACTGATTATAAATTAAAAACATCAGGTTATAATTCAGCTGAAGAATTTATTCTACAAATTAATAAAAAGGGGATTTAAATGCAATTAGAAATTATTAATCCAGAAAGCACATTAGTTAATGATGTTGTTGCACATCGAGTAGCTTTTAGTATTGGTAGCAATTTTAATATATATTGATATGGAATCATCTTTGTATGCGGTTTTTTAATTGCTATACTAACATATAGTCTACGTTTAAAATTTCATTATAAAGTGCCTTATGATCCAGGATTTTATTATATTTTTTTAGCAATTCCCATGACAATTATTGGTGCTCGTTTATGATCATTAGCAATCGGTGATGCTAAAGATTTTTTTGATTTTAGAAGTGGAGGGTTAGCGATTCAAGGCGGGGTGATTGCTGGGGTATTAAGTGCTGCAGTTTATTTTCCTCTTATTTTGCGCATGCCTAAATATCACATTCGAGATATTGATGCTGATGGCAATGTTGTTATTCGCCAACCTAGTATGTGAATATATGCGGATGCTATTATTCCAACAATTTTAATTGGTCAGGCATTAGGGCGCTGAGGAAATTTTATTAACGGTGAAATATTTGGTGCTGAAAGTACTGTTAACGATTTACAATGATTAAAAAAAGCTATGCCAGCTGTTTTCGAAGGAATGAAACATTATTTCATTGAGGGCAATAAAACTCTATTTACAATTTATCAACCTTTATTTCTATATGAATCATTCTTTAATGTAATTGTTTTTGTTTTTATTTATTTTGGCTTGTCTTACATTAAACAATTAAAAATAGGCTTTATTTCAATGTCATATTTTTTCTTTTATGGTGTTACTCGTTTTAGTACGGAGTCAGCTCGTGCTCCACAGTTTAGTTTTGAAGGAACTTATATAATTAATTCATTGTTATTAATCTTTGGTGTATTAGGTGCATTATATGTACAATTTATAGCGCCACTTTTAAGAAAAAAATTCTTATTAGATGCAATTATTGAAATGTTTTATAAAAAGAAAGACCAAATTCATAAATTTGGAGAATTAAGAAAGCCAGAAGAATTTTTATTTTATTGTCATAAATAATAAAGAAAAGGATTTTAAATGAATCAAGAAGTTTACGATTTAGTAATCATTGGCGCAGGTCCAGCCGGTTTAGCTGCTGCTGTTTATGCTAAACGTTCTGGATTAAATGTGATCATTGTTGAAAAACAATTCCCAGGAGGAAAAATAGCATTAACATCAAATGTAGAAAATTATTTAGGTATCAATAGTATTCCAGGACCTGAATTGGCTTATAAAATGTACGAACAAGTCTTAAATTTAAATGTTTCTATTATTTATGAAGCTGCTGATGAAATATCTTTAAAAGAAAAATATAAAAAAATTAAATTAACTACACAAACATTAATTACTAAAACTGTAATTATTGCAACAGGAACAGAAAATAGACGGTTAAACATTTTAGGTGAATTAGAATTTGAAAACAAGGGAATTAGTTATTGCGCTATTTGTGATGGACCACTTTATAAAAATAAAGCTGTCTCTGTGATTGGATCTGGTAACTCTGCTGTTGAAGAGGCCATATATTTAGCTACAATTGCTAAAGAAGTTCATTTAATTGCCAATAAACCACAATTTAAAGCAGAACAACAATTAGTACAAATTGCAAATAACACACCTAATATAAAAATTTATTACAATAAACAAACATTCGAATTTTTTGGTCATCAGTTTTTAGAAGGTTTAAAATTTAGAGATTTAATCACAAACGAAGTAACTACACTAAATATTGAAGCTAATTTCACTTTTATTGGCCTATTGCCTTCAAGAATTAATACAAATAATTTATGTATTTTTAATGAGGTAAACGGATTTATTACAACTGATAAAAATATGCAAACTAGTGTTTGTGGTATTTTTGCAGCCGGTGATATTGTTGATAAAAATGTTCGACAAATTGCGACTGCTACAAATGATGGAGTCATTGCAGCACTTTATGCCAAAGAGTACATTACAAGAAATAATTGATAATGATTCTAACAAATTCACGTTTAGTGAATTTGTTAAAGAAGATATTTATACTAACACTGATTATTCATCCACAGACTATAAAGTTATTTTATATTCGTTTTTTCGTAATAATTTAACCATTAAAATTGGTCAAAAACTAACTTGAATTATTAAATCTCAAAATTTACAAATTATTGAATTTATTTTAAATGGTTTAGATTATTTTAATAATTTAAATATTGAATATGAGATTATGTTTGAGCCTGACCATTTAAATAAAACTCGTACTAATTATTCGCTATTACTTTGAGGTGATTTGGATAAATTAGATGAAATTTTAAAACTATTTGATGATGCGAATAATGAAAATTTTCATAAAGACCGCTATTGTTCAAATTTTTTAATTGGTGCAATGTTGTCTGGTGGTTCAATTGCACACCCACTTGAAAACTATCATTTAGAAATTCGCTGTGATTCAAATAATTACATCCCATTATTAACAAAGGCTTTAGCCCGTTATGGTTTAGAATATAAAATTATTTATCGTAATAAAAAAACAATTGTTTATTTTAAAAAATCAGAGATAATCAGTGATTTTTTAAAAGCGATCCGTACGCAAAATTCGTTATTTGAATTTGAGAATATTCGTATCCAACGTGATTTTAATAATCAACAACAACGTTTAAATAATTTAGATATTTCTAACCTTTCTAAAAGTTCTAAAGCAGGGGTTTTGACTAAAGAAATGATTTTAGAAATAAAAAAAAACCACGATGAATTTTGCAAACAAAGTACTAAATTTTTAAAGTATTGTGAACTACGAGTACAAAATCCAGACTGTTCATTAAATGAACTTTCTTGTTTATTAAAACAGATGTTTAATATTGAAATTTCAAAAAGTGGTTTAAATCATTTTAATTCTCGTATTAAAGAAATGTATGAAGAATTAAAATTAAAAAAACTAGTTTTTAAAAACTAGTTTTTTTATCTCATTGTTTAGGATTGATTAATCTTTTTTTCAATTAAAAAGGCAAATTGATTTGTTCTTACATCTTTAAAAGTTTCAATATACATATTAGGAGTGTTAATTAATTGGCGGTTTTTAAATGCGCGATTTTTATTAATTTTAGAAATATATTTACCCATACTAATATCATCGTTAACTTCAATATCTTTGTCAATATATTGATGTAAAAATAATTTAGCATTAGTTTTAGCATCAACACGTCAAGTTGTTTCATTAACGATTTGAATTTCTTTTATTTCGTTCTCATTATCAGTTTCATCATAAACATTACCAGTTAATTCTTCAGTAATGTTTTCTAATGTAATGATTCCTATAGGATTTTTTTCAGTAATATTATCAACAACAATTCCTAAATGAATTCTTTGAAATTGCATTGTTCTTAAACCATCATATAATGTATCTTTTTGGTTTATTAATAAAGGTGTTTGCATGATAGTTCTAATGATTTTATTAGGCTCGTTAAGAAGATATTTAATAATTTTTTTTAAATAAATAATACCAACAACCTCATAGTTTTTATTGACTACAACCAAACGCGAAAATCCAGTTGAATTAAAAATTTCAAAACTTTGAAGAACAGTTTTATTTTCATTAATTTTAACAACTTGTGTTCATGGAACCATTTTTTGTTTAATTAATATATCATCAAATAAAATTGCTTTATTAATAATTAATGCTTCTTGACTATCTAAAGTTCCTTCATGACGAATAACTTCAATAAGAGATTTTAATTCTTCTTCAGTGGCTGTGATAATATTTTGTGATTTTTTATTAAAAATTCGATTTAAAGATCAAGTTAATGGCCAAAAAAGTATATAAAAGCAATTTAAGGGATAAGCTACTAATTTAAGAACTTTAATCGGATTTTTCTTAGCAAAATTTTTAGGAATAAATTCGCCAAATATTAAAACAATTAAAGTTATAATTCCTGTTGCAATTCCTGTACCAATAGCTTCAGCATTACTTGTCGTACTAAAAGATTTCATTATACCTGCAAAAAATAAACCTGCGATTGTTGATGATGCTGTATTTACTAATGTGTTACCAATTAAGATGGTTGCTAAGGTAATTGTATAATTATTAATCATTTTATAAACAACATTAGTAATTGTTTTTGATTTAATTTTATTGCTTTCAGTTTTTCATTTTACGGTTGTAACACTGGTAATAGCAGTTTCGGCTGCTGAAAAAAAGACTGAAAAGCATATCAAAATAAGAGTAATTATTGCTAATATTACTAAAACGCCAACGCTAAAATTTGCTTGAGTACTGATCGTTTCCTGGGAATCTTCAATCATAAGTTTCCTTTTGTTATAAAAATTATTATCAAGTCATAATTTGACTTCCATTTTATTATATATAAATATTGATTGAATTAAAAACTTTTATTTTTTAAAAAAGCTTTATTAATTCCATTTTTTATTATTAATTTTGATTAATTAGATATTTAATTTAATTTAATTATATCCATAATTGTTGTTTTATTTATAATTGATAAGAATTTAAATAAATAAAATTAAGGATCTAAAAATGCATAAGAAAACAAAAAAATATTTAATTAGTATGTTGGCAACAATTTCTATTGTAATTGGTACAACAACAATTATTAGTTCATGTACAAAAAAAACATCTGCAAACGTTCAAGAACAAATCAAATACGATATAATGCCAACTTATACTTCTCAGGCTGATAATTTATTAGCGTTAGGAATTACACCTGATTATTATCCTCAACAAATGTATTGAAAGAAAAATGCACCTTATGATTATTTAAATCCCCAAAAACCAAATTATCAAAAATGATTTTATGAAAAAGACGATTTTGCTAATAAATTTAAAGAAAAATTAATTAATTTAGAAAAAAATATTAAACAATATGGAACTACATGATGAAGCTTTAGTGGCAATACTTATGGCGAAGGTGTAAGTGAAGAGTATTGAAATAAAAATAAAGGCAAACTTGTTTATTATGATCGTTATTTGATTGATAATAGTCATTTTGAACGTGCAAAAAAAACATTAGTTGCACATGGTGGCCCGATATCAAATCAAAAAACTGCTATTCCTGTTGATTTTAAAATGAGTCGTGATTTTTATTTAACATTAAATAAAAATGACATTTTAAATTTTAAAAACGATCCTAATTCATTATTACGTAAAAGTCTATTATTAGGTTTAGAGTATAAGGATAAAGAAAAAGGTGAATTAAACGAACTTTATAATTATTCCTATTTTGCTGATAAATTAAACAAAAACTATTTTAATGCTGAAACATTTGATGGTATTAATTTTAATAATTCAGCTTTTAAAAAAAGAATTTTAGAAGGTAATGATATACCTATTTTTTATACTGAAACAAAATGAAAAGATCCTAAATCATTAAATTCAAAAATTTATGAAGCATTTAAAACAGTTGTGTTAACAAAAAAAGTTAGGGAATCAGGATTAAATAAATTAAATTATGATCCTTTTTTATCAACTACAAAACAAGATAGTTATGTTACATCAATTCTACAACACCATCCTATTTATGAACAAAATATGCGATGAGATGGAGGTACACAGGTTTATTTAGGAACAATGCGCGATTCATTGCTTTATTTATATGATATTGCTTATGCAACAACAAAATATGCATACTCAGAAGAAGCAAAAATTGATTTTAAAGATAATTTAGCAAAATTAGAACCATTAAAAAAAGCGTTATTTAATGCTAATCAAATTGCTAATAATTTAATTAATCGCTTAAACAAAATGCGTTTGTATTTTCAAGCAATTGGCGTTGTTGATAAAAATTATAATCCTAATATAAAACAATTTGATAATACTAATTCCAAAACTTTAGGCTTATTAACTACATCACAAAATAGTGGTTCTTCAACTTTACAAACTCAATCTAAATACGGTTTTTTATATTATGATTTAGGGTTTAAAGCACCTAAACCAATCTTAAAAAAGGACGAATGGACTGAATTATTAGAAGATGTTAACGGCAAACAATTATTATGTCATAGACACGGAAATGGACAAATTCATTGTTTTGGTGGTGATAATCAAGATGGGACACAAATTGATAAAAACATTGTTGGTTCTCTATTTAATATGGATGATAATGGTTGATGATGAAATTTAGGAGAGGGATCATTAAGTGCTAGTAACTTTGCTAAATTTAATTCAGAGTTCGACTATTTAATTAAAATTAGTTATCAAAACTTAGAAGATTTTCATAGTAATAATTTATCAAGACCACAAAAAGAATTAATCATGTCATTAATTAAATCAGATTATGATGATCCAGCGAAGCGTATTTTTGATGATAATTATGAATTATGAAATGATGGAATTAAATCACCTATTGGATATAATATGATTTTAGATTCTGTGATTAAAATTATTAATAAAACAATTGATCCGCAATTAATCCAACAAAATAAACAATTGTTAAAAGAAGCTAATGATTGGGGTTCATATTGAAAAACATTTGTTAACCAAAAATAATAAAAAAAACGAGAATCAATGAAAATTAATCAAAAAACGAATTTAAAGCAAAAGCATTTTTATTTAATTAAAAATCTTAATTATAAACAATCTGTAATCATTAAAATGGCAGGACTAATTCTCATTGCCATTTGTTTTGTTTTTTTAACATTAGCAATTTATTTTGATACCACTAATGGTTTTGAAGCATCGATCAATCAATTTAATTTTAATATTCGTGGTCCTAAAACTTTAATGGCCTTTTTTTCAGGAGGAGCAATTGCTGTAGCTGGATTATTATTACAAAAAGTTACACGTAATCATTTAGCAGATACGTCAGTTTTAGGTATTGGTTCATTAAACATCATTTTAATGACAGTATATTTAATTCTTATTAAAGATAAAACAAGCAGTTATTTAAATCCTGAAATGCGAATTTTACCCTTGGTGATGTTTTTATCATCAATTTTTGGAACAAGTATTGTTTATACATTATCAAAATTAGGAAGAGCTAATAATGAAAAATTTATTATTATCGGAATTGCTTTGAATTTTTTATTTGAAGCGATTTCCGTGGTTTTAATTAATCCTGCTAATAATTCATTGTTCTCCAATCAAGATAAAAATTTAATGATTGCTTTTGAAAATATCAAAAATTATACTTTAGGAATTATTCCAAATTCGCAATATATTGATTTATCATTAGTAATCTCTTGCTCAGTATTAATTGCATTAACATTGATTCCAATTTGATTTTTACGTAAAAAAATTGATTTATACGAAACAAGCGCTGCTTTAGCATCAACAACAGGAATTAATATTGAGCGTTTAAAAATTAGTATTTATTTACTAGTGGCTTTATTAGCAGGATTAGAAATTGTTTTAGTTGGTTATATAGCTTTATTAGGAGTTATTGGCGCTAGCGTTGCACGTCAATTATTTGGTAATAGAACAGGTATTACAATGTTTGCAGCATTTTTAATCGGCGCAACTTTAGTGTGTTTTGCGTTATTAATTTCGGTTAATTTTAATACACAAGTACCAGTTGGTTTTTTATCAACAACAATTATTACACCTTATTTTATGTATCTAATTGTGAGAGGAAAATAAAAATATGTTTAAAATTTTTGCTAAATACTTTTGCAATAATCAATTAGATCGGAATCAAATTATTAATGCAAGTAAAAACGACGCTTTAATTATTAATAATTTATCTTATAGATATCACAAAAAACTTCCTGAAGTTTTAAAAAATTTGTCATTTAGTATTAAAAAAAATTCATTTACAACAATTTTAGGACCAAATGGATGTGGTAAGTCTACAACAGCGAAAGCGATTGTTAAATTATTAAAACTTAAAAAAAATATTATTAAAGTGTTTGATAGAAACATTAATGAATTAAGTTTTAAACAATTAGCACAATTAATTTCTTATATTCCTCAAAGTATTGAAATTCCCCAAGGCACTCGTGTTATTGATTTTATTACGTTTGGTCGTAATCCATATTTAGGATTTAGTGGCATTTTAGGAAAAGAAGATAAATTAATTATTGATTTAGTAATTAATGAAATGCAACTGCACGATTTAAAAGAAAAATTTATGCAAGAATTATCTGGTGGTCAGCGCCAAAAAGTTATTTTAGCATTGTGTTTAGTTCAAGATACTCCAATTATTTTATTAGATGAACCAACTACATATTTAGATATTAAAAATCAATATGAATTACTTGAATCACTAAAAAAATTACAAATTTTACGTCAAAAAACAATTATTGCAATTTTACATGATATTAATCAAGCTATTCAATATAGTGATGAAGTTTTTGTCTTAAAAAATGGGCAAATCTATGCAAATGGTAATCCAAATGAAGTTATTACTAAAGAGTTATTAAAAAACGTTTATAACATTGATGCTCAAATTGATATTGTTGATAATCAAAAAATTGTTCATAAGATTAAAGTTAAAGATTATTTATCAAATAGTGAATAATATTTTAGTAATTAAAGTAAATAAATTAAAATATTATAATATACGTGTATTAAACTCATGATTTATTTGATAACTAAAAGGAAATAATATATGAATTTTAAAAGTACCATCGTTAATAAAAAACCGATTGATTGGAAACACACAATTGTTTTAGAAGAATTAAATGTTGATCCTAAAGCATTAGAAATGCATAAAGAAAGAATTAACACAGTTTTTGCAAAACAAACTGAAGAACAACGTTCTCAACAATTACATAACATTATTGTTCGTGAAAATTTGTTTAATAAAGCAATGACATATCTTGCTGATTTTTATGAAATTGATGTTAATGAAGAAGATATTAAAGATTTAGCACCACGTATTAAACAAGCATTTGGTGTTGAAGATGAAAAGTTAGCTTATGAAATTGCACAAAAAATCATCGCTAAAGCATTAATTTTTCAAGATTTACAAAAAGAATTTACAATTGAAATTAAAGATGAAGAATTAACAAAAATCCTTGAATCATACTATGAAGAGACAAACTTATCAATTCGTGACTTTAAAGAAAACAAAGCACAATGAGAAGCTGCAAAATCAACATTATTAGAAGAAAAAACAACAGCGTTTATTGTTGATAAATTTGATCGTGATTTATCAATTCTTGAAGCTAATATTCGTAAGAAAATAGCTGAACAAATGGAACTTGATAAAAAGATAAAGGAAATACAAGACAATAACAAATCAAAACAAAACGCTGACAAATAAAACTTACATTGTTCGCTATATTATCTTAAAAATAGCTATGGATCAATATAAAATAAATGAATTGATTCCTAGTGAGTATAAACTAGCAAGTTATTTTAATTGTGGACGAATCACTATTCATAATGCTTATGAAGTATTAAAAGTTTTAGGGATTGTAACAACAATCAAAGGAAGTGGTTATTATGTTTATTCAAGTATTGATCACTTTACTAATAAGGCTTTTGCTAATTTATACAAAACTATTGATAATATTAAATACAAACAACTTCTAATTGATTTTGAATATCATGGTAATAAGTTTAATTCAATCACTAAATTTAGTTTAATTAAAAATAAAAAAATAATTGCAGTATCATACTATATAACACCAAATAATATGGATTTAGAACAATTCGATACTAATAACATTAATATTAGTAAACAATTAATCCTCTCTGGTTTTGATGATTTTTTTAATGTAAAAACACAAATTAATTATGAAAATATTAATTTATTAAAATCAATTTTAGAAGATGAAAATATTAAATTTGACACACCAATTATTTTAAATAAATTAACTTCATCATCATTAAAAGTAGAATTATTAGTTTTAACACACATTAGCAAAGAATATTTTGATTATGAAGAGACTAATAAATTAATTATTAATTAAAATCAACCACTAAAGTATAAAATACTTAGCTTTAGTGGTTTTTAATTTAAGTTAAATCTGTTAAAAAAGCCACGCATAATAAATAAGTTGTTGCAATAATTTCATCACTACTTGCTGAACGACTAATAACACTAACTTTTGTTAAGATATTACTAATATATGTACCGACTGTTAAATAATTAAGTTTTGTTTGATAAATTTTAAAAACAGTATTTGCAATACTTATATTATCAAAAACATAATGATTAATATGGTATTGATGAATATTAGGTACTTTTTTATTACGAACTTTTAAATCAACTACAGCATCGAATTGAATTGGGCCAATAAAATCAAAATTTTTATTTTGATAAATAACATCATTTTGACTTAAATAACTAATATATCCCACATGATTGTTTAAAACATCAAAATAATTTCTACTAATTGTGCATGAATATTTTGCTACATCAATCATTTGTTGTTTATTCAAGTTTAAATTTAAAGCACAATCACTCATAAAAATTAATTCTCGTTTATTAATCATAACCATTGTACTATATGCGATCGAACCGTTAGGATTTTTCAAAATCTTTAAGATACTACTAAAACAAGTTTTTGATGACAAGTTAGCACCCACAATTAAAACATCGATTTTTTTTAAATAACTTAAACAAACAGCAAAATTTAAATTGTCATTAACTGCTATTTGGGCATCATTTCGACTCTCACCTTTTAATTTTCGTAATTCATATCAAATCTCTTCACATTCATTAATAATTTCTGGTGTTTTAAAAGATGACAAATCTACGATATTAATTAAAGTATCTTGACTTAACATTTTTTTTGCTTTATCAATTTCTAAACTTTGTTCAATAAATAAAACTTTTATTGGTTTTTTTGCTAGATTTTTTAATTTATTTTTTAACTTATTTATAGAACTCAACATGATTTTTTTCCTATTTTTTAGCAATTAACTTTATATATTGCTATTAATAAATATTATAATTATTATATTCGTTATAAGGAGTTTGTAATGGAAAATAAAAAATACGAATGAGATCTTGATGATTTATTAAACAACAAATCATTAGATGATTTATATGCTGAATTTGAAGAAAAAAAAGCTAAATACATTAAAGCTTTTCCAACTTTTTTAGATTCAAAACAAAATTTTGCTCAATGACAAATTTTAGAAGAAGAATTCACAATTGTTGCAAATCGTTTTTATAATTATGTATCAAATAATTTAAATACTAATGTTGTTGATCCAAAATGAAATTCTTGATCACAAAAACTATCAGCTTCTTTTTATGAATTAGAAACAGCATTAAGTAATTATAATAGTGTAGTTTTAGCAAACGAAGCAAAGATTAAAGAATATTTGACAGATTCTCAATTAAATGTATATACACGCAAGTATAATGAAATTTTTCGTTATCAACCACACACATTAAATAATGAACAAAGTAAGTTATTTTCAACATTAATACGTGCTGATGAAGGTTTTTCAACAATTTTTTCAACATATACAAATAATGATATGAAATTTAGTGATGCTATTGATGCAAAAGGTAAAAAACACCCAATTAAGAATGAAGCAGAAGCTTTTGTTCATTTAAAATCAAAAGATCGTGTTTTGCGTAAAAGCGCTTATTTATCAATGTATAGAGCATATTATGATTCACGTGAATCAATTACAAAAATGTTGTACTACAATTATTTATCATTAAATCAACAAGCAAAAGCAAAAAACTTTGATGATTATATTGCTAAAGCAGCATTTGATGATGTTGTTGACAAATCATTAATTACTTTAATTTATGATCAAGTAAAACTTTATAAAGATACAAATGAAGATTATAAAAAAGTACGAAATACATATTTAAAAAAATTAATTAAAGTTTCAAAGATCGAACCTTGAGATAATGGACTACCATTAATTTCTAAAAAAATTGACATTCCAATTGAAGAAGCAAAACAAATGGCTATTGATTCATTATCAATTTTAGGTGATGAATATGTAAGTAATATTAAACGAGCATTCGATGAAAAATGAGTTTCATGATTACCACAAAAAGGGAAACGTGGTGGGGCTTATTCAATTGGTGGCACAAAGGGTATTAGTAAATATTATATTTTAATGAACTATACTAACTCATTACGTGATGTACAAACTATTGTTCATGAATTAGGCCACTCAATGCATTCGTTATATTCTAATCGCACACAAAAAATTTATAGTGATTACAAGATTTTCTATGCGGAAATTGCATCAATTTCAAATGAAGTTTATTTAAACTACTATTTATTAGAAAAATACAAGAACGATCTTGAAATGAAATTAATGATTTTAGATGAAATGATTAGTGGGTTCTTCGCAACAACAACAAGACAAGTAATTTTTTCAAATTTTGAATGAATTGCTAATGAATTAATTAATAGTGGGGCACCATTTACAGCAGATGTTGTTATGAAAGAATATCAAAAACTAGAACTTGAATATACTAACAAACCAATTGTAGAGGATCTAAATTCAATTTATTCATTATCAAGCATTACTCCACTTCGTATTCCTCATTTTTATGTAGGTAATTTTTATGTATATAAATATGCAGTAGGTCAAGTTGCAGCAATTATTTCAGGTCATCGTGTTTTCACTAAAGTAACAGGTGCAAAACAAAAAGTGTTTGATTTCTTATCAAGTGGCGGTTCAAAAGATCCATTAGATACAATTAAATTACTAGGTGTTGATTTAACAAAACCACAAGCATGACAAGAAGCCTTAGAAATTGTTAAATTATGAATTAAAGATTATAAACAAACAATTGCTTTACTAAATAAAAAAAGAAGCAAAAAATAATATTTAAATTTTCAAAAGGTTTAATATGAGTGACGAACAAAAAACTAAATTAATAGAATAGAAATTTCATTATTGTAATCAAATTAATTAAAATCTATTTTTCGTTTTTCATAAAAAAAGATTGAGTAGTTAACACACCCAATCTTTTTTTATTTTTAACTTTTGGATTCCAAAAATAAAAAAACTAATAAAAGCAAATTTAAATAATGTTATGATTAACACATTAAATTTTTAGGAGTAAATTCATGAGTAAAAAAATCAAAATTTATTTAGCTGGTCCTTTATTCACCTTAGCTGAAATTAATGATCGAAAACAACAAGCTAGTTTAATTAGAAAAACATTTAAAGATGAATTACCAAATTATGAACTTGATCTTTTTAATCCGATCGAAGTTAACGACGAATTAGGTGCTAATGCACATAAACCAAATATCTTTTTTTATGAATCAGATATTAAATTTATTGATCAAACTGATATCGCAATTATAGATATTGATAACACTGATGATGGCACCATGGCTGAAATGGGTTATTTTGTAGCACTTCAAAAACACGTTAAACCAACGTTAAAAATTTATATTTTAAATACTGATTGAAGAGTTCACAAACACCGCAATGAAGTTTTAAACAAGTTTTTAGATGGTATGATATTATCACATTGCCAATATTTTACTAATTTTACAGATTTACTTAATCATTTATTAATAGAATTAAAAAAATAATTCAAATCAATCAATTATATATATAGGCACTAGTGGTTTTTAAACTCACACGCCTACTTTTTTACTATCTAATTGTGATGAATTATAACATTGCACTTGATAGTTGTATTTTTTTATGATAGTTTTAATTTATCATATTTTTTAATTAGTATAAACTTTAACACTTTAGGTTTAACAATTGGAAATAAAAAAAATACAAAATGTTTGATTTATTAACATTTTGTAGTATTGTTATTAGTTTTTATATATCGCCTTTAAAATATTACTAATTCCATTTCAATTTTATTTTGTTTTATCTTTCATGTGAGGAAATAATAAAACATTACGAATACTATCATTACTTGTTAACAGCATTACTAAACGATCAACACCGATTCCCAAACCACCTGTTGGAGGCATACCATATTCTAAGGCCTCAATAAATTCCATATCTAATTCGCTTGCTTCGTCATTTCCTAATTTAGCTTCTTCTAACTGTGCGATAAAACGTTCATATTGATCAATTGGATCATTAATTTCACTATAAGCATTAGCAAATTCTTTTTGACAAATAAATAATTCAAAACGATCTACAAAACGAGGATCACTCTGATTCTTTTTAGTAAGTGGAGAAACTTGTAACGGATGACCATATACAAATGTTGGTTCAACTAATTTTTCTTCAACAAAAGTTTCAAAAAAAGCATTAACGATATGACCAAAGTTTTTTTGATGATCTAATAATTTAACATGGTGTTTTTTTGCTAATTCAATTGCTTGTTCATCGCTTTTAACTTCAAAAAAATTAACACCAACATATTCATTAATTAAATCAACCATATGAGCTTTTTTAAAAGGTTTATTTAAATCAATACTAAAACCTCTAAATGTAGGATTTAATAAGTTTAATTTAGTAGCAATATGACGAATTAAATCTTCAGTTAGTTGCATAATATATCATAAATCAACATATGCAATATATAATTCAACTGAAGTAAATTCAGGATTATGAGTACTATCCATTCCCTCATTTCGAAAAATTCTACCTATTTCATATACTTTTTCAAAACCCCCAATAATACACTTTTTTAAAGCAATTTCAGTAGCAATTCTTAAATAATATTCTTTATTTAAAGTGTTATGATGTGTAATAAAAGGACGAGCAGCAGCACCACTTAAAATATCTTGCAAAACAGGCGTTTCAACTTCAAAAAAACCTTTATTATCTAACCAATTACGCATTTCACGTATAATTAAAGATCGTTTTATAAAAGTATTTTTTGTCTCATCGTTAACAATTAAATCTAAATAACGTTGACGTGCTTTAATTTCTTCATCAACCAATCCATGATACTTTTCAGGGAGAACTTTTAGACTTTTAGCAACTAATCGAATGTTTGTAACATTTAAAGTAATTTCATCACTATTAGTTTTCATTGCTACACCTTCTAATTCAATAATATCACCAATATCTAATGATTTAAAAGTTGAAAAAACCTCTGGAGCAACATTTTTTTTATTAATATAAATTTGTAACTTTGCTGAAAAATCTTGAATAATCCCAAAGGTTTGACGTATTCCGATTAAACGACCAGCAAGTGTCAATTTCTCTAAATCCATATTATGTAATTCTTCTTTGCTAAAATTTTTATATTTTTCAGCAAAGTCTTTTAAAAACATACTTCTATCAACTTTTGTTATTAAAAAAGGATTTTTATTTTGGTTTTTTAATTCTTGTAAGTGTGTACGACGGATTAATTCTTGATCACTAAATTTTCTTTCCATAAATAATTTACCTATTATTAAAATACACTATCTTTTTATTATCGCATATTCAAAATTTTAAATAAATATAAAGTGTTTTATTATTAATTTTTTATATTATTTATAATAATTAATTTAATTGAAAATAAAGTTTATATAAAATTTTTAAATATAAATCTTCAACATAAATAACTTCGCAATCAAAATACTTAAATTCATTATTAATACGATGGTCATTATTCATTGAACGGCACAATTCATTTAAAATCAAATCAAGCAAATAAAGTTTATAAATAGCGTGTTTTTGACGATCAAAAACATTATTTAAATTATTTTGATCAATAAAATAATCGATTGGAGCAAGCATATCGCTTTCTTTGCTACGATGCGCGTATTCATTTAAATACTCATGAAATGATAAGTTTTTACCACCGAATTTATTTTGTTTTATTTCTTCAAAGACCGCATCACATTTTTGTTCTCTAAATAAGGTATAAGCGAAATTAAAATTTTCATAAAATTTATAATCATCCCAATTACCTAATTGGTAATCGTTAACAATCTCTTTTAATATACTAAATTTATTAGCAATTCATTCATTATCTTTAAAATCAACTATATTTAGAATTTCTAGTTGTTTAAAGACCTCTTTTTGAACAGGTTCATTTAATAAAACAAATTCATAATGAATCTTTCTTGTATGTTTATCATGTTTATATAATTTATAAATAAATTGACACAAAACAATAAAATCTTTTTCATCTAAGTTGTTAATAATAAATTGTTTTGCTTCTTGTTGTAGACGTTCAATTCTACGTTCGCGTTGTTTTCTTAATTCAATAAAATAAATATTAAAAGAAATTGATAGTGTTGCAATTAACGAAAGTATACTAATTACTAACGTGGTTATGTCTAACGATGTCATGGAATCTCCTGATTGTTATATATGTATTTAACTACCTTTATAATATCATACTATTGCAATTATTCATTAAAATTTGCATAGTAAAGTTCTTGATAAAAAGGCGAATTCTTAATCAAATAATCATGATTCCCTTGTGCAATAATTTCACCATCTTTAATTACAAGAATTAAATCAGAGTTAATAATTGTTGATAATCGATGGGCAACAATAAATGATGTTCGTCCTTTTAGTAAATTATTAATTGCTAATTGTAATTTATGTTCGGTTTGCGTATCCACAGAGCTTGTTGCTTCGTCTAAAATAATAATTTTCGCATCAGAAATAATAGCACGAGCGATAGTTATTAATTGTTTTTGACCTTGTGATAAGTTTTGTTTTTCATCAATAACTGTAGCATAATCCTTTGGAAATGAAAGGATCAAATCATGAACTTGAGCTTTTTTAGCTGCTGCAATAATTTCTTCTTTTGTTGCATTCATTTTACCTTTTTTGATATTCTCATAAATACTTTCACTAAATAAAAAAGGATCTTGTAAAACAATAGAAATATGTTCACGCACACTTTCACGGGATTTATTTGTAATGTCAACATCGTTAAAATAAATAAAGCCATTATTAATATCATAAAATTTATTAATTAAGTTAGTAATTGTTGTTTTTCCAGCACCTGTGGGTCCAATAATAGCAATTCTTTGGTTCTTTTTGGCAACAAAATTAATATTTTTTAAAGTAGGCGTATTTTTTAAATAATTAAAATTAACATTTTCAAAACGCACTTCAATGCCTTGAGAGGCATCAATTATTAATTTTTCATTTCAATTTATCTCATTAGGAGTTTTAACAATTTCATCAATTTTAGCAAGTGATGTTCTAGCAACGATAAAAATAGGAATAGTATTTGATAATTGACTAAAATTATCACAAAAGAAATTACCAAATAATGAAATACTAACTAAACCACCAAAAGAAATAATATTCGTTGTTGGATCAATATTATTTATAAGCCCTGGCAAATGAATTCCTTTAATAGAAATTAATAAATAAGACACAATTAAAACGGCATTCATTAATCGCATAGAAAAGATACTTCAAGGAAAAATAATTTGAGAACTATATTGAGCTTTAATTCAATTTTTATATAATTTTTTAATCATATTATCTAATTTTTTTATGAAAAAAACTTGTTCATTAAAATTAGCTACAACGTTTTGCCCATAAATCATTTCTTCAATATATCCGTTCATTTCACCAAAATTTTGCCGTTTTATGCGCATTAATTGATGGGATTTTTTAAATATCAAAATATTAAATAGCATTACTAGAACATAAATGACAATTCCAATTAAAGCAATATAAGGACTTGTTATTAATAAAGCAGTTCCAAAACCAATTGCGCCAAATATAGCTTTATTTAACTCAATATAAAAATTAACAATTCCATCTTGTACACTTGTTGGATCACTTAGTGTTTTAGTAAAAATTTCTCCGCTTTTATTAATATCAAAATACTTCATTGGTAAATAACGAATACGATTAATCACCATCATTGATAAATGGGATTCAATCTTAAAAGATATTCGAATGGCAAATTCGTTGATAATTAGTAAAAATATAAATGAGAAAAAATAAATTCCTATCAATCCTGCACACATTTTAACTACTGTATCAACAATACCATTAGCAATAGCATATTTATCAACCGTTTCTTGTAATAAAAAAGTTGCTGCAATAGTTGTTGATAATCAAAAAATAATGGCAATTGACAAACCAATAAATACTTTTTTAAAATCCTTAAAATAATAATAAATTAAATAAAGTTTACTTACAGGTTTATTGGTTTGTAGTTGTTGGCTCATATTCTGCTCCTTTATTTATTTGATGTTGATAGATATTTTTATAAACTGCACAAGTTTTAAGTAATGTCTCATTATTACCAATTCCAACAATCTTACCTTTATCCATTACAATAATTTTGTCACAGTCTAAAATATTATTAATTCTTTGAGCAATAATAATCGAATTCTTAAATTTAATTTGTTGTTTTAGTGCAGTTAAAAACTTTTTTTCAGTGATCATGTCTAATGCGCTAGTTGAATCATCTAGCACTAAAGTTCTTGCGTCGCGCAACAATGCACGTGCCATTGCTAGACGTTGTTTTTGTCCTCCAGATAGATTTTGAGCCCTTTGTGCTATTAGAGATTCTAATTTTTCATTCTTAGATTCAATAAAATCCTTAGCTTGTGCAATTTCAATAACTTCGTTGATTTTGTTTTCATCAATATCCTCTAAACCAAATAATACGTTATCACGGATAGTTCCATAAAAAAGCAAAGGGACTTGTTCATTAAGGCTAAAAGCAGCTTGCAAACTTGTTTTTTTAATATCTTTTAAAGGAATATTATCAATAAATATTTCTCCGCTATTTGGTTCTAATTGATGCATCAGTAATTTAATTAATGTCGTTTTTCCAGCACCTGTGGTTCCAATGATTCCTACGCGATCAGTGTCTTTAATTGAAAAAGAAATATTTTTTACTGCTCATTTTTTTGAATTATGATACCTAAAACTTACATTTTTGAATTCAATATTATTGTTATTAATTTCTTTTGTACCATTATAAACAATTTGATTTTTATAAAAAATTAAATCATTAATTCTTTTAATACTTACTATAGATCTAAAATACTGTTCGCTTCCAAAACCAATTAACGCAATTCCTAAAACTAATAAATTAGTTGATTGCATGATTGATAAAATGTTTGTTGCTTTCATAGAATTAGTACAAATTCCAATAATTAAAATAACCGGAGTAATAACATAAATGATAAAAGTAACAATAGCTATGCTTACTTTACTAATTCGATTGGCAATAATAGATTTAGAAACATTATCTTTTCATTTAAAATCTAATTTTGTTAAAAAAAATGCATCTAATAAGAAAGAACGAATAACTTTTACTCCACTAACATTTTCTAATGACACTTTTGCTAATTCATCATTAGCAACACGCATTTTATTTCAATTTTTAGCATTAAATTTTGAAATAATCATAAAAATAATAATTGTTGATAAAGCCATCAAAACTACAGTTAAAGGGATTTGTCATAAATTACCACTAATAATGAATAATAATATAATTGCTCCAATGAAATAAAAAATTCCCATAATTACAGGTCGTGTTAAAAAGATAATTGTTTTTGAAAGTTCTTCGACGTCTTTTATTGAAGTTGTTAATAAAATTGATTGATTAAATTTTTGTTTATCTGCATATGTTAAGAACATTGTTTTTTCAAAAATTTTCTTTTGTAAACGACTTTCAACAATAATTTGAAAAACGGTTGAAATGTAATTTGTAAAAATAGAAAATATTGCTCATCCTATAGCCATGGCGATTAACACACCGCCATAAATAAAAGCGTCAGCGGTTGTTGATGCATAATTACCATTATTAAGAATTGATAATAAAAGAGGAGAACCAATTACTAATAAAACTTGTACTAAATTTAAAAATAAAATAAAGAAAAATAAAAATTTATGTTTTATTAACAAAATCTTTCAAACACTTTTCATTATAATTCCTCCTTTAATATTAAAATAAACGCGTTTCACGCGCTAAATCTCTTTTTTTTATATCCTCACGTTTATCATGAAGCTGTTTACCTTTTCCTAAAGCAATCTCTATCTTTAGTTTATTATTTTTCCAATAAATTTTGGTTGGCACAATTGTCATATGTTGTGTTTTCACTAAATGTTGAAGTCTAATAATTTCTTTTTTGTGTAATAATAGTTTTCGATTCCGGTATGGATCTTGATTAAAGTTATTACCCTCAAAAAAATTTGCAACATGCATATTTAAAATTAAAGCTTGATTTTTAATTATTTGGACATAAGCTTCATTAATTGAGCAATTAGCACGTGAAATAGATTTAACTTCAGTTCCTTTTAAAACGATTCCACATTCAAAAAAATCTAGTAATTCATAATTTCGACGTGCGTGTTTATTTGATACAATCATTATTAAAAATACTAAAAACCTTTCTTTATTAATAAATTGTAAAGTTTATTAGTTCAAAAGAAATTTTAATCGCAAAAAAGTTATATTTTATATAAAAATGTAACATTTTATGATAATTAGCAATTAAAAAACGACTAAATATTTACTTTAGTCATCTTCTTTGTGTATTATAAATAAATTTTTTTAAGTCTTATATTTTTTATTGTGCAACTAGTTCAAAATCAATTTTTCTTGTAAGTTTGTTAGCTTCAATAACACGTACACGAACTTTATTGCCAACAGTAATAATTTTCTTTCTTTTTTGACCAACTAAAGTCATGTTTTCAGGAATAAAATCATAAAAATCATCTTTTAAATTTTTAATTCTAATTAAACCTTCAATCGTATTTTCGAGTTCAACAAAAACACCAAAACTAGAAACTGTTGTTACCACACCAATAAATTCTTTACCAATATGTAAATTCATATATTCAGCAAATTTAGCAGCATTAACATCACGTTCAGTCTTAACTGCTATAATTTCATTTTTATTTGATTCTTCAGTAATTAACTTCAGTTTATTTACTAATTCTACTCGTTGTTTATCTGTATAACTTTGAGAATCAAAAATAAACATTCATAATAAACGGTGTACAATTAAATCTGGATAACGCCGTATTGGTGATGTAAAGTGTGTATAGTTATCAGATGCTAAACCAAAATGTCCCGTATTAATAATTTCATAAGAAGCTTTAGCCATTGATCTTAATAATAATTTACTAATTAATGGTAAGTTAACATGATTATCATTCATTTTTAGTCAATTTGAAATAGTGTTAGGTTGAATGTTCTCAAAATCATGTGAGATATTGAAACTCAATTTTTTTGCCTCAATTTCAAATTTTTGTAAGTTAATAATTGATGGTTTATCATGAACACGATAGATAAAAGGCATTTCTAAATTAAATTCTTTTAGTGTTTTATCCATGTGTTCTTCAGCAAACATAGTTACAGCCTCATTAGCTGCAATCATAAAATCTTCAACCATCATTTGAGCAGTTTTACGTTCGTAAATTTTAATATCAATAGGGACACCAGTTTCATCTAAAATAATTTTTGGTTCTTTAATATCAAAATCAACATAACCATCTTTAATTTTTTTATTTCTTAAAATATGGTGCAATTCTTTAGCTTCTAATAACATTTCTTTCATAGTTAATGTTGCTGATTCAAAACGTGATTTTCCTTCATAAAAATCATTAACTTCATCATATGACATTTGACGATAATTTTTCATGATAGCAGGATAAACATCTTTTCAACATATTTTTCCATCTTTGTCAATTACCATATCACATGTTATACACATGCGAAACTCACCTTCGTTTAATGAACAAATATTATTAGAAATATTATGCGGAAGCATTGGAATAACACGATCAACTAAATATGTTGATGTTCCCCTTTTAAAAGCATCAGCATCAAGAATGGTGTTAGGTTGTACATAAAAACTAACATCAGCAATAGAAATACCTAAAAAGTAACGTTGATCATTTAATTTTTTAACATAAACAGCATCATCAATATCTTTAGAACCAACAGGATCAATAGAAATAATTTCACGATCAATTATTGAACGTCGAATTTTATTTTGATGTTCATCAACATAAAATTCTAATTTTGAAGCCAAATCAACAACTTGTGGATCAAAAGTCGGATCAATACCATTATCATAAACGATTGATAAAACATCAGAACCAACATCGTTTTTATGACCTATGATATGAACTAATTCTGCAATAGCGTCTTTTGTTGTTTGCGAAATAATTTTAAAAAGAATTTTGTGACCATTAACTAAACCAGTAGTATCTTTTAAATTTATATTTAAGTAAAATAAAGGATCGTCAACAAAAATGTAATAACCACCATTAGGAAGAGTTATAAATTGACCAACATAATGGTCTTTAGCATGCCCAACAATTTCAATTACAGCAGCATCTTGCAGGTTGTTTTTAGGTTCTTTTTTTAACTTTACAAATTTAACTGAATCTTTTTTTAAAGCCCCATTTAAATATTTTTTGTTGACGTAATATTCAATTTCAGTATTATCTTCTTTAATGAAGCCATCACCTTTTGAATTGATAGTAATTATTCCTTGTTTGATCTCGTGATCAAATTCATAATCTAGATAACCAATAACCAATCGATTATTTTCTAATTTTTTAATAGTATTTTCTTGAATTAATAAATCAATTAGTTTATAAACATCAGTTTTATTTAAAGTGGGTATTTTTTCTAACACTTTTTTAGCAAGAATAGCAGCGGGTATTGGGCGCTCTTCCTTAGAAATTACTTCGGTAATAGTTTGTTTCGTAAAATCACTCATCTAGCTCACTCTATAAAAAATTATTATTAAAATCATAACAATTGTTAATAAAAACATAAAGACTTGTAAACCTTTAATTCAACCACGGTCTTTTGTTTTTTTAAAAATTTCTAGATCTTGTCCATTTAAACTTGACAATCCACCAGATGGTGAAGTTCTTGATAATAATAAACCTATTATTAAAGCTAGAACACTAAACAAGATTAAAACAATTGTTAATGCCATATTTTTATTCCTTGATAATTAATAACTATTATTATAACATTTAACAAAATGATAACCCCTAGTTTAATTTTTAAACACGAGGTTATCATTTTGCTTATTTAGTGTCGTTTTTATTTAAACCTAAATCAAAGAATGTAGGTGCATGATCAGAAATATCGTTACGAATATAAAATAATTTATCATGATATTCATTAGGGTATAAACTACGCACTTTTTTTAACCATTCTTGATCAACAATTTGATCTTCATATACATTTCATAAATTATAAATACTTGGATTAGAAATAGTTAAACCATTAACTTGAGAAAAAATTTTATCATAAGGGTTTGAAAATTCACCAGGATTTACACTTAATGATGATTTAAATAAGTCATTATCGCTAAAAACACTACTATAACCATTTTGAATTAAAGTTCTAAAAACTCATTGTTGATTATGTAAACGAATATTAGTATCACCTAAAAAGAAATAATCATCATCCTTATTGTTATCAATTTTTTTAATTTCTTCCATAACTTTGACTAATCGTTCAGCATCGTCCACTTCACGACTTCCTTGTTCATATTTATTAATCATTTGGTTCGTTGAAATTACTTTTTTATTAACTAATTCTGTACGTTTTAGTAGATGCTCTTTCTTTTGATATTCTGTATTTGATGGATAAGTTCCAGGACTATCAAGATGACCAAAAACCAATGTAAAATCATTTTTAATTTGACCAATTGAAGCAAATTTAATAGCAAATGGTGGACGTGAATAATCAGTTTCTGCTTTTTCATATTTGCCAGGTTGTAATGGATTTGAATAAATATAACCGATTTTATTATTTGCAAACGGAATAGGTTTAACTTTTTTCTTATTATAAATTATCCCAATTCGTTCAGTACTTGTATGTTCGTTTGTTGCTAATTTATCATTTTCACTACCTTTAAGATTATCACTAATTAAAAAATCATAATCATCTGAGCCACTTAACTTATTTAAATACTTAATGATTCCTATCAACGGTAAATTGTTTTGTTCCTGATTGTTATTTTCTGCAGGCCAAATTTCTGTTAATCCAATAATATCGTATTTATTAAATAAAATTGTTTTAGCTAAAGCATAGTTCTTAGGATCATTTAATCCAGTCTGATGTAGAACATTTCAGTGCCCAATTCTTAAATGATCGGCTTGTTTATTATTAATACTTTCATTAATATGATTGTAAATAAGTGATTTATTTTGGTCTGTTTCATTTTTTTCATAAAAACGATCATCATTTTTACTAAACACACTTTGATCATATTTTTGATCGATTTGTGTTTCTTTTGGTAAGTATGTTCTTAGTAAATCACGAATATTAGTTTGGTCAGCGTATGTTTTATTTAAAAGAGCCTCACTAAATTTTTTATTAAATTTTAAGATCGAATTCATAACACTTATTATAATTTTTTGATTATCATTTAATTCTGTATACGTACCATCTGAATTTTTCAAAAAGACTTTAATAACTCTTTTTTGATGACTTAAAATTAGACCATCTTTTTTTTCATTATCACTATAACTTTGTTCTGATTGTTTTTTTCTTGATTTTTTCTTTGTTTTTGTTTCTTGTAAGTTTTCATATTCAATTTTTAGCGTATTACTAAATTGATATGGTTCGTTGTAATTTTGAGATTTATTCTTGTTAATTAAAAATTCTTTAAGATTTTTAACACTAATTTCTCCTGACACTAACGTATCATTAAAAGGTAAAATTTGATTTAAATTTTCTAGTTTAATAGGTTTAGCAGAAACCAAAGAACGAATAGCATGAGGAGCTAATAGGATAGCTTGCGTTTGTTTAAATTCAAAAGTATGTGTTTGTTTATTATAAAAAGGAGCTTGATAGTATTTTGCTAATATATCACTTATAAACGCTCCTAAGGAACTAAGTGAATATGTTGAAGAATATAAATTGACTTTTTTATCATTTTTAATCAGTATTTTTTCTCTTAATTGCTCGTATTCATGATCTTTATAATTATTAAGAAAATTATTTAATGTAATCAAATTTTGATTGAAATCAGGATTTTTTTGTGGATTTAATAAATCTTGTTGAGTTATATAATGATATCCTGCATTATCACCGCTTGTTGACAGTTTATCAACAAGCTTAATTTCTGTTTTTAAAATACGGTTATAAGGATTTAGTGTGATCATATATCGTACAAAACCACTATTAGGTTTTGATTGAACAACATTCAAAGGTTGATTATTTTTATTATTAGCAATTACATTAAGTGCTTTGTAAGGATTAATAACACTCATTAAATTGACATTATTATCTATTAATTTACTCATTCGTGTTGCTTCTTTATAAAAAGATTTTTCATCATTAGTTAAAGTGTTATTTTCCTTTGCTTTATCTGCTAAAAAACTTGATAATAAAACAATATTTTTAACACCTTGACCCTTAAGCTTATTGGCTTCATCATTAATCAAATTAGCTGTTTGTTCGTTACTAAACCATTGAATATTTTTTGTTAAATTAAGATTAGATTCAAAATTAATTAAATTACTCATTGCTCCAATTAATCCTAATTTAAAACCACCAAAGTTAATAATTTTTGAATTATTAGTCATTTGTATGTTTTTATCACCATCTTTTAGATAAGAGTTAACAGATAAAATACCATCATTACCATATAAAGCTTTGACGTATTCTTTAAAATGCTTTGTTTTTTCATCAATTAAATCATAACCCCAATTTAAATCAGTATTAGCGATGCTAGTAGCTACTGGATTTAAAAACTTTAATGCGGCTGTTGTATATTGAGCTCTAGTTAATGAAGATATATTATTTTCATAATAATTATTTCCACCCATAACTACAGCAACATTATTCGCACCTCGTTCTTTTTTAATTTTATTAACTTCATTTAATAATCGAATTAAACCGGGTGCTTTTAAATATGTAGTTTGTTTATTATAATCTGGAACAATTTGTTCATCAACAAAACCATTAAACCCACTAATAATAATTAGTTCAACAGTTCTAGCGATACTATCATCCATTTTAGTTTCAAAACTAAATGTCTGATTGTTTTTTGTATAAATAATTTGATTAGGCGCTTTTGGATCGTAAAAATCAATTTGTTTTAGTAGATATTTAACATTCTCATCTAAACTGTCAAAAACAAATTTTTTATCCACTAAATTTGTTTTAGCTGTTTTAATTGTTTTTAATCAGTTATTTTTTTTATCAAAAACATCAAAAACAGCAACCATTTGTAAATTTAACTCTTTATTTATGTCAAAATCCAATGTAAATTTTGCTGTTTCTTTATTGAAGACATTATTTTTTAAAACAATATCAATATTTTTAGCTTCAAGATTTAAATTACTTATTTGTTTTGGTTTTATAACTTCTTGCTTTATTTTTTTCTCTTTTGCGCATGTAACTAAAAAAGGAATTATTGCACTAGTTAAAACAGTTCCAATAGCAATAAAACCAAACATTTTCAATTTATTTTTTTTACAAACCACTTTATTTTAAACTCCTTAATTTGTATAGACAAGTTATATATAAAAATCTTAACAAGCTATAAGAAAAAATGATGAAAAATTTATACAAATATTTATAATATTTTTAAATATCTAAAATTAGCTTTTGAAAACTTTTTTAAAAAAAGATGTTTTATTTTGCACTTTTGAAACTAAACAAAACATATTAATGGTGAACATTTTAATAAAAAACCTCGGTAATTAATATAGTTACCAAGGTTTAAATTTAATATAAATTAATAATTATTAATGATCTTGAGAAATATCCTTATTTGAACCAATATAGGCAAAAACATCTGGACTTTGTTTTAAATATTTACCTTCTAAAATACGAATTACACTTTCAACAGTCTCTTTTAATGGAACAAACTGACCAGGAGCTTTTGTGAAATGTTCAGTCATAAAAAAGTTTTGTGTAAAGAACATTTCTAGCTGTAAAGCTTTTTTAACAATAATTTTACTTTCAGCATCTAGTTCATCAAAACCTAAAATTAAAATAACATCTTCTAGATCTTTATAAGCTTTTAAAACTTTTTTAGTTTCGATAATTGCATTATAATGACGTTCACCAATAATTTTAGGATCAATAGCGTTTGTTGAGCTTGCTAATGGATCAAATGCTGGAAAAATATTTTTAGCAGCTTGTGCTCGCGATAATACTAATTTTCCATCTAAATGATTAAATACAGCGACAGCTGATGGATCACTTAAATCATCCATTGGCAAAAAAATTGTTTGAAATGAGGTAATTGATCCATTTTTATTTCTAAATAATCGATTTTGAATATGTGTAACATCACTTTCTAGTGTTGATTGATAACCTCCAACAGACGGTTTTTTACCTAATGCAGTAGAAACTTCATTTTCAGCTTGAATAAAACGATAAATATTATCAATAAACAATAAAACATTTTCTTTGTTTTGATCACGTAAATATTCAGCAGCAGTAATTCCAATTGGTACAATTGAAGTACGTGCACCAGGAGCTTCATTCATCTTAGAAATAAACATTACTGAATTAGGCATTAAATTAGAAGCAGTCAATTCATTATATAATTCAATTGCTTCACGTGAACGCTCTCCTGAACCAATAAAAATATTTGAATTTGCTTGTTTATGTCTGTTTAAGTTAAAAATGATTTCTTTCATTAAAACTGTTTTACCAACTCCAGCACCACCAAAGATTCCTAATTTATATCCTTTTAGAATTGGCATAAAAAAATCAATTGCTTTAATTCCTGTCTCTACAATTTCTATACTTTTATCAACATATCGCTCGTTTAAAATTGTTGAATTCATTTCAACATATTTAGGTGTTTTATTAGTTTCTAATAAAGGATGTCCCCAAAAATTATAAATATTATTTTTTGAATCATTACCAACTGGGACCATAAAACTTTTGTTTGTATTAATAACAATATCATTAATTGCTAAATCTTTTGATGCATAAACAACAATTGCTCTCGCATGTGTAGCATCAACTAATCTTTTAACTAATAAGAAAGTATTGCCATCATGAGTTGTTAATAAGTGATTTAAAGCTGGTAAGCTGTTTTTAAATTCAATTTCGACAATATCTGATCAGATTTTTATAACTCGGCCGTTATTCATTATTTATCTCCTAAAAATTTTGCTACATTCATTAAAAACTCATTTTGTAAAGGAATAAAACTATGTTCTAATTCAACTTCTCAATTTAAATTTAAGTAATCAGAGTATTGTTTTAGGATGAAAGCAAAGTAGTTTTTCATAATTTTATCATCATAATTTTGAGTTGTTGAAATAGTATTGAATGCTTTTTGAGCATCTTCATAATTATTAATCGCATAATCTAACAACATAAATGCTTTTTGTTCATCTTTAACACCTTTTAAAATGCCTCAGGCAATTAGCTTAGTACTTAAAACAATAAAACGGTATGAAAATAAAGAGAAACCTTTTTGTAAAAACATTTTATCTATTAATTTACCTTTATGCAATAAAAGGCTAGTTTCTTTATTAAATTCATAATCTAACATTGCTAATTTTAAATGACGTTTGTATTGTCTAAAAATTTTATTAATTTCACCTGCTACTTTAGTAATCATTCTTGATTGTACACTTGAACCTGTTCTTGAAACTGATAAATCAATATTAATAGCAGGAACTTTACCAGCAGCAAATAAATCAGTTGAAGTTACAATTTGACCATCAGTAATAGAAATAATATTTGAAGAGATTAATGAAGTAATATCCCCATCAACTGTTTGTAAAATCGGCAAAGCAGTAATTGTTTTACGATTTTTGAATGAACCAGCACGTTCAAGTAATGATGAATGTGAGAAGAATACATCTGAAGGCATTGCTTCTTTACCAACTGGTTTATTTGTTAATAAAGCCATTTCACGAATAATATTAGCGTGTTTTGTTAGATCATCAAATACAATTAATACATCATCTTGATACGATAAGTTTTCAGCATGAGCCATTCCAATATATGGTGCTAAATATTGTTCATATGCTGTTAAAGGAGCATCGATAATAATTGTGTTTTTTAATGCGTCATGAGCCTTTAAAGTTTCATAAACAGTAGTTAATGATTCGCGTTTTTGACCAATTGCTACATAAATACATTTTGTGCCAGCACGGCTTTGATTAATAATAGTATTTAAAGCAATATGCGTTTTTCCAGTTTGACGATCACCAATAATTAATTCCCTTTGTCCTTTGCCAATCGGAATTAATAAATCAATTGCTACAATTCCAGTATTTAATTGCTCATTTAAAGTTTTCACTGTCATTAAATTATGAGCTAAACCAAATGCAGGATTAGTTTGAGCAATTTTTGCTGATGGAAGATTATTAAGTGGTTTTGGTCAAATAAAATTATTATTAATATCTATAATCTTCCCGAAATAATTTAAATCAGTAACAACATTTTCTTCACGATTTGTTTCAATGATTTCATCGCCGATATTAATATTAGCATGTTCATTGCTAATTAATAAATAAGCAAGATCTTCTTTTGCTGAAATAACAAATAATTTTACTGTTGGATCATTTTTTAAAACAAATATTTGATGATGTTGATAATCATAGATTCCACTAACAGTTACAATATAGTTAAAAATCGATTTAATACGAGGGTTTGCCTTAGTGTTTTGCATTTTTCTATTTCCTTTCTAAATTATTAGAATAATTCCCATTATTAAAACAATGATTGTCAACACCATTATCACACTATTAATAATTAGAATAACTTTTTTATTTTTTAGTTTTTTAATCAATCCATAAATAATTAAATTAATTATTAAAACCAGTAAGCTAATTACTATAAACGCAATTCCAATCTTACGACGAATTTGTTTATTTACTTGTTTTTGTCTTTCAAGAGATTTTAAAGCACTATCATATGCATTTTTAAAATCCTTAGCTTTGTCCTTATTTGTTTTAATATCGGTTTGATTTGTTAATATAGACATTAAATCAAAATTTTCTTTAATCGGTTGTAAAACATTTAAATATGACTGATATCATTGTTGAGAATTAAAACTACGTTGTGCAACAATCGCCGATAATTGATAAATTAATTGGTTATTTAATTTATATAAATTTGCAATGGTGTTTTCATCACCTTTAATACTTGCAATATTAGCTTTAATTTTATCTTTATTTAATCGAAGAACTTCACTAAACTGTAAGCTAACTTGTTTATAAGCACCAACTAGTTGATATCAATAATAATCATTATTGATTAAAGTGTTATTTAAATAACGAAAGAAATTTTCTTTTGTTAAATTATTAAACTCATTTAATAATCTTTCCTTATCATCGGTGTTGTTTACAAGTTTTTGTTTGTATTTTTCTAAATATTCATTTTGTAATGTTGCAAAAGTTTTTTGACCAATAATTTTATTATCTTTATCAATTTGATTGAATCGCAAAATAAGTTTTTGAGCAGCTTCAATCATTGTAAATAAAGCATTTCTAATATAAAAGTTACGTAATTTCTTATAATCAATTTTCTCATCTAGTCCTAAATGTTTATACAAGTTAACAAAAATTTGTTTAATAGCATTAACTTCATTTAATTTTAGACTATTAAAATTAATATCTAAAATTGGATCATATTTATTAATAATATATGTACGTTGTAGTTTAGGATTAACTTGATCAATAATTTTAACTTTAATATTAATCATACTACTACCATTAACGCCAAAATCTAATACACTATAAAGATAACGTGTGTTAATTGGGTTATTAAAATAAAATAATGTTTGTTTAAGCTCTGGTTTTAAACTATCAAATTTTGATTTTAATCCACTTAATGATTCATTTGAATATATAGGACTAATATAAGGTAATAATAGTGGTAAAGAAGAAATAATAGCATTAGCTTGTGTTGGGGGAAGATCTATTTCATTATGATCGCCTTCTACTAATGGTGGTGGTAATGGTGGTGGTGTATCGGGATCTTTTTGCTCATTTGGCTGTTCTTCTTGTTTATCTTCGTTTGTTTGTTGATTTTGTTTTAAATCAAATGCTGTAACACGATCTTTTATTTTAGAAATAAAATAAGCGTTTCAATCATTATAACCTTTAGGTAAAGTTGTATTAAATTGTCCTTTTTCATCACGTGTTAACTCAACATCTTTGTTAATTTGTGGAACATCATTTTCATCATAAATTAATTTATTAATTTCTTTTAAAAATTCAGATTCATATTTTTTTAACGTATCTTTGTATTTTGTTTCTTCAACATCATTAATTTGATCACTTTGTACATCACTAATTTTTCCTTTTGGAGCAACGGCTTTTTGATAATTTAAAGGATCAGTTTTGCCAACTTTGATTTGCTTATATGTTTCTCCATTAAACTCAATATCTTTAATATCATAATTTTTAAGAGTTCCTAAAATTTGAGGAGTATTTAAATAAAAACCATAATCATTAGGATTTTTTAAGATGTTTTCTTTATTTTTCTCAAGATATGACTGCAGATTTTTTAAATAAATTAGTTGTTGAATTTTTTTCTTAAACTCAATTTCTTTATTTTCTAAAATTTCTTCTTGACGTGATTTTACATAAACAATAGCAGCATTAATTCCTTTTTCTAAACTATCTTTAACAGTTTTATCAGCATCAGTTTTAAAAGTATTAAAATTAGGATCGTTTTTAGGAATTTTTGGTTTTTTTTGTTGATTAGGATCATGATTATTACCATTATTGTTTTCTTGTTTACCATCCTCATTAGCTGCAACAGAAAAAATTAATGGTGAAGCTACAATTGGTATTGTTGCTAGTGAAGCTAAAAATTTTAATTTTATTTTCAATTTATTTCTCATCTTTTTTATACTCCACTAAATTTAATCTAATTTGCATATTAGTTATATATTGTTCTTGTTTTAGATTATCAAGATCTAAAAATTCACTTAGAGTTAATCCTAATTGTTGTTTAGCTTCAAGTTCTAAAATATTATTAGTAATATTTTTATATTTATCTACCAACTCTTTTTTATGATTATTTTTTTTGTTTAATCCATCTTTAATAAAGAAATGTAAATCATTTAATGCTTGAATTTTAACGATGTTATTTAAAACAGAAATATGTGTGTTTTTTAGAAATAAATAAAAAGTTTTTTTATAATCATCATCAACTATTTTTAATAAGATTATTTCATAGGCTAAAACGGTGTTGTTTTCAATTTTTTGTCATGTATTTTCTTCATTAACATTTACATATAATAAACCATTATCAATATTAAACTTTTTGTTTTCAAGTAATGTAATTTGAATATTATAATTATGATTCATAAACACCACCATCGTTATCATTATCATCTTCATTCATATTTTGTCTTGCTAATAAATTTATTTCTTCAATTTGCAACTCTGTCTTTACACGTGTAATTTTACGTTGAATTTTTGAAAGGTTATCATCTAGTTCTTTTAGGATATTATTAGTAGCTACTAGTCCATTTTTTGTTTTATAAAAAGATGATTCAGTAATTAAAGTATTAATAATATTAACTAAGAAAACATTAATTTGGACATTAATAAATTCATTTAAATTTGGATAAATTTTTACTTTTTGAAAATCAATAATATTAGAATCGCATTTTTGTAAATTAATAAATTTATCAAAACTAAATTCATTCATTGGTAATATAGTAAAGTGTCCATCATAGTTTTTATTAGAATTAATAACAAAATTAACTTTTTTATAATTATCAAAAGTATATAAAATCATAATCATTTTAGTTAATTGTGTAGTTAAATTTTTAATATTTGAGTCATTAAATGTTTGCAGAACATTTAAATTATGATTCTTACCAAATTCAATTGCTCCTTGACCGATCAAAATAAAATCATCACGATTTGATTTAATCGTTTTTAAGATTTTTTGTTCATAACGCGAATATGAATCAGTACTATATTTTTGCTCTTCAGTTAAATAAATCCATAAAACACGATCATTAATTTTATTTAAAAACTTAAAAATCTTTCGTTCCTCATGTCTTAACATATCATGTTTAATATTATAAATACGCTTTAAACTTAGAATTGATTCTTTTATTTCAAAAGCATTTTTACTAAAAAAAGTTAATCGCTGATTTAGTTTCATAATATTAATTAAAAAAATATTACGTTCGTTACTCACTTTTAAATTAATATTATTTAAATTCTTTTTTTTATTAATTAAATCTTTTAATTGCATACTTAATTTCCTGATTTTTGTTCAATTGAAGCGATAATTTTTTCACTAAATACGAGCACTAAACTACCATTAAGATTTGATTTAAATAAAATCAAATGAGGAAGAATTTGAACAGGATGATTTTGAAGTAATAATTTTTTAATTTTTTGGTTATATGAAATTAAAAAATTATTATAAACAGGATTTTGATCATTTCTAAAATTAATTGCATCAGTTGTAGAATTAGCTTTAGTTACTAATATTGGAATCATAATAGTTGTTGAAAATTTTAAATTTAAATTGAAATTAATAATAAAATTTTCAAATCAAATATCTAGTTTTTTTTCAAAAGCTAATGTAAATTTTTTAACAAAATCTAAATAAACCTCTGATTCAAAACCTAATTTATTATCAAATAAAACTGATGCCATTAATTCTTCATATGTAATTAAATCAACACTTGCTTTTTTCTCTTTATAAGTATTAAATACATCAAAATAATTTGTTGTAGGTGTCAGATTAGATTTATTTTTTTTCATTGTTTAACTCCTTATTTAGCTTATTGATTTTAAAAATTACCATTGCAAATTTAATTGTTAAATAACTAATTAAAGTAAAACTCAAACTAAAATTTAATAACAACATGATTTGTACTAAATTCATTTTCGGTTGAATGTTTGAGAATAAATAGTTATTATGATGATCAGATGTTAATAAATAATCAAAACCTACAATTGCTGCGGTTAAAATAATTAAAATCATAATAACCTTTAATAGCACCAATAATTTAGTTGAAATGTTTTTATTTTGAAATATGTAGATTCCAAACACACTCATTTGTACAGTAATTGTAATAAAAACATTAATTAAAGAAACCACACTCTTAGAATTAAATAATGTTACTAAAAACAAGCTTAAACTTAGAATTGTTTGAGTGACTACTAAATTTAATAAAGTATTTAAACTACTCTTTACGCTTAAGCGTTTTTTTAAAGTGAATACCATATATAGTATTAATATGATTATTGCAAAAAAACTTTGTAAAATATAAACTCATAATAAATGGTTGTTACCAACCGGTAGATTTTCATTTTCGTGCTTATATGCAAAAACTCTTAATAATCAAATAAATATAGCACTAAATAAAAGTAAAATAAATCATAAATTATTCTTAACATAATTACGATCGTAACGTTTATTAATTACTAAAGCGAAAAAATTAGTATTTGCTAAAACAATAAATGTAATTAAAAAGAAGAAGAAAGCAATTAAAATAATAAAATTTTTACCTGATTGAACAGTGAAGATATCAACTATGCGTGTGTAGAAACGATTATCAACAAATAAAAAGTTAGGACTTTTACCTGTATTAATTCATACAAGGGGGGTAATTATTACAAATAATAGTAATAACGCTTGAAAAACCATGGCAATAATTAAATTTTTATAATTATCGTATAATAAAGGGTTAGTTTTTTTAGAAATAAAATAATTAAATATTTCATAACTTAAATTTAATAAAAATAACACTAATAGTAAAAATACTAAATTAAAAACTTCTTTTGGTCAAACACTAAAAAAAGTAAAAAATAGTAAGAAACTAATTGTTGCAATCACTAAATATAAAACGAATCATGGTAAGTATTTTTTAATGGTTGCTCGATGCCAATAAAGATTTGTGAAATTTTTGATTAAGGCATAAAACAGTGTAAAACTTAAAATAAAAAAGCGAAATACAATTAAAAAGTTAAGTTGTAAATATTTAGGGCTGTGTAAATCAAAAAAAGCATTTAGCGATTTTGAAATCATCATTGATTTATTCTCATCACCAAAACCTAAACCAAACAACGTATTTTCAGCAAAGATTAAAAGTAAAATAAAGAATAATAAAGTAATAGCAGATAACAATCCTTTTAAAAATTGATTGATAAAGCTACTTTTTTGTTGTTGCTTTAGCGAATAGTTATCATCATAAATTACTCTATTAACGGAAGTAAATGTTTTTGTATTCATCATATAATTTTCTAATTCAATATAAAAGTTAATTAATTTTTAAATTTAAAAGCACTGGGGATTTGATCTTCACCAAAACCATTAGGGAATAAAGCGGTTGCAATATTTTTATTTTTATATAACGCCATCATTGCTTGGCGATAGGAAGTTTTTTGATCTTTTCCACCACCATAAATCAAATCATATTGTGGTAAGTTATAACTACCATATAATCCATGATAATCATAGCCTTCAGATCGAAAAGCAGCAGCCAAACCTGTTTGGGCAAAACCATTACCAACATGCCAAATTCCCACTAATTCATTTTTTTGATTACGTAGACTTGTCCCTGATGAACCCCCAATTGGAGAATAATGCATCGGTAAATAATTTAAACCAAACCCAATATAATTTTGATTATCAGTTGATTTATGTACAGTTTTACCAGTAATAGGACTAGCAATAAAAGCATCAAGCAATCCAGGTTTATTAGTAAAACTACGATAACCAATATTATATGACAAGAAATCACCACGATTTATTTGTGTAAGAGGAAACAAAGGAGGATTATGATTTAATTTATTATAAAATTGATAATTAGAATTAATTCATAAACTATAACTTTCACGTCTTGATTCAAATTGTTTCTGATCTTTAATTGGGTCTAAGAAATAATCAGAAGCAGAGCTTGGCCAACCTACAGCATATAATTCATCATATTTATTTGTGTCAAAAGCCTGATTGTATTTTGAATCCAAAGGTACATTAATACGTGCATAATCATTTAAATAAGATTTTGATAAAAATTTTATTTGTTCATTTGGTTTTTGTGCATAATCGTTTGTGATTAATTTAGCTAATTCACTTGGATCTAAATTATTATATTTATCAACACTTCAATCTGATCCACTATAAAAATTTTTCGTTATAGAGCCGAGTTTTATTTTTTCAAAATCAATTTCAAAAACAGCAAAATCAACAAATTCTTGTAGGTTATGGTATTTATTTTTTAATTTTGATGTTAATAAGTCACTAGGATTTAATTTTAAGAAGTCACGACCATCATAGATTGTTTTAATACCATTACTAATAGGTTTATCCATATTTGGTTGATTATCTTTACTAACTAAACTAAAACGATAAATATTATCATCAGCGTTCGCTAAACCCAAAGTAGTTTTAGTCTTAACTGTTGGCATTATCTTATTAATACCAAAAACTGTTGTTGTTGACTTTAATGCTTCTGCAACATGTAAATTTGTGCCAAAATACCATTTAGTAGGATATTTATTATCATTTCTTTTTTGATAATCCATAATTCACATTGTTCCAGCTACTACATCAGATTCGCGTGGTTTACCATTTGGCGTTTGAATTTGAATGCCAAATGTTTGTAAAGCAATTTGCTTATACATATTATTAGGCAAAGTTCTAGCCAAACCATTTATTTTCCATTGATCTTTTCCAATTGCATCAACTCACGAAACACTTTTTCCAATTTCAGTTTCGTTAAATGATAAACCTTGGTATTTACCATCTTCATCATATACTGGTAAAGTAAAACCTTTACGAGCAGCAGATTCATAACTATCTTGAAAAACTTGTGGTGCTTTTTGATCAAATTCATTAATTTTTTGTTTATTATTAATTAAATCTTGGCGTATTTGGTATCAATTTACATTTCTTTGATATGCTTTTAATGATTTTAAATAATTTTCATTATCATATAAATAACGTGTTTGTTGATCAGCATTATTGTATTGATTAATTTGAGCTAAGCTAGTTGGTGCAAATCCGTTATTAACTTGCGTTGGCAATAAATCATATTCATCAGCACCATAAGGATTAGTTTTAAATTTATCAACAGGAATAAGAATTTGGTAAGTTTTTTTACTTTTAAGATCTAAAACCTCTAATAATAAAGAAAAAGCCCCCAACTTATTAGCGTTAATTTGCCCACCCTCGTTAATGAATAAAACATCAAGTACACTTAGTTTAATTTTGTCTGTATACTCTTTTTGAATAACTTTTAAACCCATTAATAATTGATTTTTAATTTGTGAAGGTAAAACATTTTCATAATTTAATACTTGTTGATTATTAATTACGAGATCAAAAGTTTTTTTGAATTGAATAGTATCAATAATTTTTTTTAGCTCAGGCGTTAATTGATTTGAAAAAATGGTGTTATCTGGTTTTTTAAAACCATTTAATTCAAAATAATAACCTTTAATCTCCTTTGTTTTTTGGTTAGTTAACTTATAATAAATAATTAAATCCTGCAAGGAATTTTGTTTAGGATATAAACCTATAATTTCTAGTTTAAAATCTTGATTTTGAATAGGTGAATGTTTTAATTGATTAATAAGTATATTTTTTAAATAAGTATTAGCTTTATTTTCATAAGTAAAATTAATTAATTTGGCTATATTATCTAATTGGGAAGTACTTATTAAATTTGATTTAAATTCGTGGGTTGTTAAATTTTGATTTAAATCATTAGTAAAATTATTTTTTTTATTATTACCATGGTCACTACTATTATGCACAATATTTTTATCTTCATGTTTATTATTAACACAAGACGCTGCTAAAGCTGAAACAAACACAATTGGTGTGATTATACTAACGCTTGCAATAAGTTTTTTAATTTTCATAATAATTCCTTCTATTAAATATCAATATAGTTAGCGTCAGAAGCATCACTTACTTTATATCCTAAACCTTCTAATTGTGACTTTAATTCTGAATTATTAGAATCAACAACAACAGATTTAGAATCTAAACTTTCTGCTAGATTAAATAATGTAGCTAAATTTTGAATACCACTTGCAGTTAGACTACCATTGCCTTTAATGTAAATACGTTTTGTAGCATTACCATTACTAAAAGTAATTTTACTTTTAGGAGGCATTGGTTCATTAGTCACAATATGTTCACTAAAACCAGCTTCATTTAAATCATTAATTGACATTTCATAATTTTCTTTATCATTAAAGAAATTAACTCTTCTTAATTTACGTTCACGATTGTTTGAAGCTTTTTGTTCGTCTTTAAATATTAAACCCCGTAATGAGCGAATTTGAGGGACACGACTAAAGTCTAAACCTTGGGGATAACTATTTCCCACCTCTTTATGATCAGGATCTAATCCTGGACCAAATCCACCTTGAAAGAATGGTTCATTATTTCTTGTTCAATAAACCATTCGTAATCCATCATTAATACGCTTTAATTTTGTTCGAAGATCATTTGCATTATCATTGAAATCATTTTTATCAAAAGAAAGAGTATCAAAAGTAATTCGTGTAGCAATATCTGATCCTTGTTTATATTCTGACGAAACATTATAATCATTAGAATTAATTCATTCTACTTTTCGAAGCGCATTTGGATTAATTGATCATTCATCTAATAATGAATTACCTAATGTATACAACCCAAGTTCTTTAATATGTTTAGTTTCAAGAGCAGAAAGTGCTGACGTATTATGTGAACCAGCAGAAAAGAACAATTCAAGCAATGGCAATTCATTTGGTAAGGCTTTTAGAATATCAGAGAACTTTTGTCCTGAATCTGATTTCCCCATGTTACGAATTCGATATCCTGTAATTTTTTTGTTTTGATTATTTAATTTTTGAATTAATTCTTTTGTTTTTTGGTATCCATCAGGATTATCAGCATCAATATCAACAATATATCCAGTATTTAATTTACCTTCTTGTGGTTTTTGGCGCTCTAGTTTATAAACTTTAATACCATCACCATCTTTAATGCCATACTGTTTGAACTCAGCTGTTGCATCTGATTTATTTCAACCAGGATAATCACCATTAGCTAAACCATTTGGACTACGATTGTATCATTCGTTATACCCAAACACCCTTCGGTTAGCATTATCATTTGCTAAACGGCTTGTTACTGTATTATATTCTTTAGCAGGTGAATAAGCATATGAGTCTAATTCGCCGTTTTCATTAACATATGAATTATCTGGATCAGGCGTTAAACCTTCTTGTAAATACTTTTCAGAATTGGCTGAAAGTTTAGTGAATTTTGAAAAATCTAAATGAGCATATAATCAAGCATACTTGTGCGCATCAGAAACAAATTTTGTTTTCATATTAGGATATTCTTTTTTACCTTGTTCATTCAGAAAATTAACAACATTGTCTGTATCAAATAGTTTTTTAAATTTACTTCATAATTTAGCGTGATATCCACTTTTATCAACCATTGCGATATAACTATCAATTTCCTCTGGTTTTATATTTGGATCTAATAAATCTTTAACTAAACTTTGATAATCGCCACGCTTTAAATTCCCGCGCACTAAATCTGCTGATTTGTGCCGCAACTCATCTGTTACAAAAACTTTTTTAATTTTCCCAACACTTATATTTGTATAGGGATTAAGATTTGATAATCCATTATCCTGATCATATTTAAAAGTTTGACGAGGGGGTGGAACTTCAACTTCAGCGTCCACTAAAACACCATTCAATTCAATTTTTCTAACAGTACTTTGGTTAGGAATTTGTTGTGGTTGTGGTTGTGGATCTGGTTCTGGTTTTGGTTCTTCTTTTGGAGGTGGTGGGCTTGGTGGTAATAATTCTTCTTTTGGTTTAGTTTCAACAGGAGGTGTTGGTTGAGGAATAGGCTGAGGTTTTGGTTTAGGAATTGGTTCTGGTTTAATTTCAGGCTTTTTAGGCTCTTCTGGTTTAATCTCTGGTTTTTTTAACTCTTCTTTTTTTGGAGGTGTTGGGATGGGATCTGGAAATGGTGTTGGTTGTGGTTCTGGTTTTGGCTGAGGTTTTGGTTTGGGAATTTCTTTTAAGTTATTATCTTTAATTGATGGTCTTGCTTTTTGGTAATCTAAATTGTCTTTATTAATAAAATTTTCTTTTTGATCAATAGAAGCAATACCAAAACTTGAAGGACTTTTTTGACTTGTACCATAAAAAATTCCTCCTAATAAAATTGAACTCACAGCAACTGCACCAATTGTAATCGTTAATAATTTTGTTTTACGTTTAATAAATTTCATATACCAACAATCCTTTCAATTAAAGATAATTTATTTTTAATTATTATTAAATTTGAATTCATCAGGAATCTTATCGAATCCATCAGGAAATAATGCTGTTTTAATATTGTTATTTTGATATATATCCTTCATCGCTTCTCGGTATGATTTCTTTTCGGTTTGATCTTTTCCACCGCCATAAATTAAATCATATTGTGGTAAATTATAATTTCCATATAATCCTTGATAATCATAACCTTCAGATCGAAAAGCAGCAGCTAGACCTGTTTTTGATGAATCATATTTAGCATGATAGATAGCAACCAATTCATTTTTTTGATTGCGTACACTTGTTCCAGAAGCACCACCTGCTGGTGCAAAATGTTTGGGTAAATACTCTAGTCCCATATTAATATATTTTTTATTATCACTGGATGTGTATAAATTATTCCCAATTTGGGGAACTGCAATAAATGCGTCAACTATTCCAGGTTTTTGAATAAAACTACGGAATCCAATTGCATAAGATAAATAATTACCTCGTTCAGTACGTTCTGGCGGAAAAGCAGGCTGACCACCTTCTTGTTGAGTTAAATTATTAAAAAATCGGTAATCAGAATTTGTTCATAAACTAAAATTATCGCGAGTACGGTATTTTAATTGGTCATCATCAACATAAGCTTTAAAGAAGAAATCTTCTGTTGAATTAGGTCAACCTAATGCAAATAATTCATCGTATTTTTTAAATCATGTTTTAGCGTCTTCTTGTCTATATTTTAAAGGAACATCAATTTTAGAATAATCACTTAAATATGATTTAGATAAGAATTTAATCTGGTTATTTTTATTATTAGCATAATTACTTGTAATGACTTTAGCCAACTCTTGAGGATTGTAATTATTATATTTTGTTACTAACCCATTATTTTCACCTAAAAAATTTTTTCAAACATTAACTAATTCTAATTTTTCAAAATCTAATTCAAAAACAGCAAAATCTGTAAAATTACCAACATCTTTATATTTTTCTTTTTGTGAATTAATCAAATATTCAGCCGGTTTTGTTTTTAAAAAATCACGTCCATCAAAAATTTTTTTAAGTCCATGGTTTAAAAGATTTTTGCCATGTTCTTTACTTCGAAAACCAAAATTATAAATATTCTCATCTAAATTACTTAATCTAAAAGTAGTTTTAATTTGTGCAGAATCCATTAATCGCATTAAATTAATCGATAAAGTATTTTCATTTATCGCATCTGCAACATGTAAATTTGTAGCAAAATATCATTTTGTAGGATATTTATTATCACTTGTTTTTTGATAATCTAAAATTCATGTTGTACCTGCAGTTTTATTATAAGTATTGTTCTTGTGTGAATCGTTATTTAAAATTCCTAAACTAAATGTTTGCATTGCTTCTTGTCGATATTTTTCATTTGGCAATGTTCGTGGTAAACCACCAATTTGTCATTGGTTACGTCCAATTGCGTCAACTCATGACGGCCCTTTTGGTGTATCTTTACCTGCTAATGCTAAACCACTGATTTTGCCATCACTTTCATAAACAGGTAATGTAAAACCTTTATAAGCAGCAGATTCATAAGAATCTTGACCCACGTTTTTTGCTTTTTCATCAAAATGTTTAATATCACTAACAGTTGCATTTAAATCAGGGCGCAACTCTTTTCAATTTTTATCATTATTTTGGCGTTTTAAAATTTGAAGATATCCTTCATTATCAATTTCATAACGTTGCAATTGAGTTTTTGAAAAATAATCGTCTTGATTTTTAGGTTTTAAACGTTCTGTACCAATTCCAGGAATTGTTCCATTTTCATCAACACCATAGGGATTAGATTTAAAGCCAGAAACCTCTAAAAGTTTATAGTAAACTTGATTATTTTTTTTATCAGTTAACATTAATGATAATTTTAAAGTACCATTTTTATTTGCCGAACCAAAATCACCATCAGGATATAAAAAATTAATAATTTTAATATCTAATTCATTTTGATATTTGTGTTGTTGAATTTCAATACCTTGATAATAATTTTTATAAATTTGTGATGGTAATAATTGGTCATAATTTTGATTAGGAAAAGCTGCTAGTTTAAAATCAAATGTATCTTTTAACCTGATATTATTAACAATTTTTTTAAAATCTTCGTTTGCTTGACTTAAATCATTAACGATTTTATCTTTTTCATAAACAGATTTAAAACCTTTAATTTCATAAGTATACTCTTGAATTTCATTCCCTTTTTTTAAAGTGTATTTGACAATTAATACACTTTGATCTTTGTGACTAACAAAAACGTCTTTAACACTAAAAACAACATCATTTGGTAAATTTAGTGGTTTTTTAATTTCATCTTTTTGCACACTTGATGCTTTAGTATTAGCTTTGTTATTAAAATACTCTAATTTTAAATTTGAAGCAATCTTATCTAAAGATGATGATTTTTTAACTTTAGTTTTATTTGTACATGAGGCAATAACAATAGGAGTTAAAATTGTAATTGGTAATATTAAAGCGAGAGTAGTTAACTTATTTTTTTTCATACATTTTTGTCCAAAATTTTTAAATAAAGATTAAATATCTATTAGTCCAGCGTTAGTTGTTGATTCAACACTATATCCCGCATTTCTTAGTTGATTTGCAAGTGGTGAGTTGGTATTATTTACAACAATTGTCCGTTGTAAACTTTCAGCAAAATTAAACATTGCTGATAAGTTACTAATTCCATTTGCTGACAATTCTTGATCACCATCAATTTGGATTTTAGTTGTACCAGAACCATTGCTAAAAGTAATTTTACTTTTTTCACCAGGTTCACCACTTACAATATGTTCGTTAAATCCGGCTTCATTTAAGTCCTCAATAGACATTTTATAAGTTGTGCTGTTATTGTAAAAATTAATTCTTCGTAATTTACGATCTTTATTATTTGATGTTTTTTGTTCATCTTTAAATATTAAACCCCGTAATGAGCGAATTTGAGGGACACGACTAAAATCTAATCCTTGTGGATAACTGTTGCCTGATTCTTTGTGGTCAGGATCTAAACCAGGTCCAAAACTACCCTGAAAAATTGGTTCATTATTTCTTGTTCAATAAACCATTCGTAAACCATCATTAATTTGTTTTAATTTTGATTTAATGTCAGATGCATTGTCATTATAATCACTTTCATCAAAAGCTAAAGTATCAAAAGTAATACGAGTAGCGATATCTGCACCTTGTTTATAATTAAATGAAACATTATAATCGTTTGAATTAATTCATTCAACATTTCGTAAGGCATTAGGATTAATTGATCATTCATCTAATAATGAATTTCCTAGTGTGAACAAACCTAATTCTTTAATTTTTTTATCCTTCAGTGCGATTAATGAAGAGGTATTATGCGAACCAGCGGAGAAAAACAATTCTAATAAAGGCAATTGATTTGGTAATGTTTTTAGAATATCAACAAATTTTTGACTTGAATCTGATTTCCCCATATTATGAATTCTATACCCTGTGATATTGATATTTTTTTCTTTTAAAGTTTGAATTAATTTTTTTGTTTTTTCATAACCTTGTGGATTATCAGCATCAATATCAATAACATATCCTTCATTTAAACGACCATCTTCAGTTTTTTCACGAGTTAATTTATTAACTGTAATTCCATCACCATCGCTAACGCCATATTGATGGAATTCTTGGGTTACATTTGTTTTCTTTCAACCAGGATAATTACCTTCAACTAAATTTCCAGGAGTACGTCCATACCAACTTTTATAACCGAAAACACGCCGATTTCAATTATCATTTTCCATTCGACTTGTTACCGAATTATAACCTTTGGCAGGTGAATATGCGTGAGAACTAATTTTTCCATTTTCATCAACATATGCATTATCAGGATCAGGTGTATAGCCTTCTTTTAAATAAGATTCTGATTCATTTGATAATTGTGTAAACTTTGTTAAGTCTAAATGTTGATAAAGCCATGCATATTTATGGTCTTTTGAAACAAATTTAGACTTCATTTCAGGATATTCTTTTTTACCTTGTTCAGTTAAAAAATTAACAACATTTTCAGATTCAAATAATCTTGAAAAACGATATCAAACTTTTGCATAATAACCGCTTTTATCGTTTTTTACATATTTTTCTATTTCCTCTGGTTTAAGAGTTAATAAATCATTAATTAAATGTTTGTAATCTCAACGTCCTAAATTTCCTTGGACCAATTTACCTGTTGCTTTTCGCAATTCATCGGTAACCTTTACGTTTTTAATTTTACCAACACTATCATTAATGTATGGATTAGGGTTAACCAGATTATTTTGTTTATCATATTCAGCAATATCACGAGGGGGAGGGACTTCAACTTCAGCTTCCACTCTTACACCATTAATTTCTATAATTCGTGAATTACTATTGTTTGGAACCTGTTGTGGCTGTGGTTGCGGATTTGGTTCTGGTTTTGGTTCTTCTTTTGGAGGCGGAGGGTTGGGTGGTAATAATTCTTCTTTTGGTTTTGTTTCAATAGGAGGGGCTGGTTGAGGAATAGGCTCAGGTTTTGGTTCTGGTTTAATTTCAGGTTTTTTAGGTTCTTCTGGTTTAATATCTGGTTTTTTTAGCTCTTCTTTTTTTGGAGGTGTTGGGATGGGATCTGGAAATGGAGTTGGTTGAGGCTTAGGTTTTGGTTGAGGTTTAGGTTTGGGAATTTCTTTTAAGTTACTATCTTTAATTGATGGTCTTGCTTTTTGGTAATCTAAATTGTCTTTATTAATAAAATTTTCTTTTTGATCAATAGAAGCAATACCAAAACTTGAGGGACTTTTTTGACTTGTACCATAAAAAATTCCTCCTAATAAGATTGAACTAACAGCAACTGCACCAATTGTAATCGTTAATAATTTTGTTTTACGTTTAATAAATTTCATTTTTATTATTTAAACCTAACCTTTATTTACTTTGATTGTTATTAAATTTAAACTTTTCATCATATTGGTCAAAACCTTTTGGAAATAATGCTGTTTTAATATTGTTATTATTTTTATATATTTCTTTCATTTTTTCACGATATGATTGACCTGTAGCTTGATCTTTTCCACCGCCATAAATTAAGTCATATTGTGGTAATTCTTTGTATTTATCACCATATAAGCCTTTATAATCATATCCTTCAGAACGAAAAGCAGTTGCTAAGCCGGTGCTAGCAAATTCATTACTTACATGATAAATTCCTACAATTTCATTGTTTTGGTTTCGCACACTTGAACCAGATGCACCTCCTGCTGGAGCATAATGTCTAGGCATATATTCCAAACCCATATTAATATATTTTTTTCCATCAAGAGATTTATATAAATCATTGCCAGTATGAGGAGCAGAAATAAAGGCATCTAGAACACCAGGTTTATCGATAAAACTACGGTAACCAATCGCATAAGACAAGTAATTACCACGTTCAGTTCGTTCTGTTGGGAAAGCGGGTTGCCCACCTTCTTGCTCTGTTAGTTTGCCAAAAAAACGATAATCAGAATTTGTTCATAAACTAAAATTATCTGTTGTACGATATTTAGTTTGATCATCATCCACATAACGTTCAAAAAAACCATCTCTAGTTGAATTAGGTCATCCAAGAGCAAATAATTCATCTTTTCCTTCGAATTTATATGATGTAGATCTTAGAGGAACATCAATTTTAGAATAATCATTTAAATATGATTTGGATAAGAATTTAATTTGTTTATCACTATGGTTTGCATAATCACTTGTAATAATTTTTGCAAGATCTTGTGCAGAAGAATGATCATATTTAGTAATTACACCGCCATTTGAGTTAAGTTGATTGTTTCAAACATTAAGTAATTTTAATTGTTCGAAATCTAATTCAAAAACTGCAAAATCAACAAAACTACCTGCATCTTTGTATTTTTCTTTTTGTTGTTCTGTTAAGTATTCTGTAGGTTTAGTTTTTAAGAAATCTCGACCATCATATATTTTTTTAAGACCATGATTTAATAAAAATTCGTTATTTTTACTTGCAAAACCAAAACGATAAATATTTTCATCTAAATTACTTAAACGTAAAGTTGTTTTAGTTTTAACACTATCCATTAATTTCATTAAATCAATAGAAGTTGTTTTATCTGTAATAGTATCTGCAACATGTAAATTAGTTGCAAAATATCATTTTGTAGGATATTTATTATCATTTGTTTTTTGATAATCTAAGATTCATGTTGTTCCTGAAGATTTATCTCAAGTATCTTTTTTTCCATCTTTTAAAGTTAAAGAACTAATCCCTAATGTTTGTAAAGCTTCATCACGATATTTTTCGTTTGGTAAAGTTCGTGGTAAACCAGTGGTTTTTCATTCGTCACGACCTCTTGTATCAACTCATGAACGAGCTTGGCCGTAACTACTTTCATTAATTGATAAACCTTTAAAATCACCATTTTTATCATACACTGGGAGTGTAAAACCTTTATAAGCAGCAGACGCATAAGCATCTTGACTTACACCTTTAGCTTTTTCATCAAATTCTTTAATTTTTTGTTCATTATCACCAAGTTCAGGACGCACTTGTTGTCAAGTTTTACCCATATGTTGGCCTTTTAAACCATTTAAATAAGCTTGATTATCAATTTCATATCTTTCTAATTGAGTTTTATTAAAATAATTATTTTCATCAACTTTAGGTTTAACAAGATCAGCACCACCACCTGGAATTGAACCATTTTCATCTAAACCCATTGCATTAGTTTTAAAACCATACGCAATTATTGATTTAATGAAAACTTGTTTTGTTTTTTTATCAGTTACTAATAGTGACATTTTTAAATTACCTTCACGATTAGCAGAACCAAAATTTTCGTTTGGATAACTAACATCTATAACTTCAATACCTAAACGATCTTGGTTAGTATTAATTCTAGTTTCTACACCTTGTGCAAAATTGTTAACAATTTGTGAGGGTAATAATTTATCGTATTCACCAAAATTTGAGTTAAAAAGACCTTTTAGTTTAAAATCAAATGTATCTTTTAATTTAATGTTATCAAAAATCGTTTTAAAATCATGATCCATGTCATTTATATTAGCGATATTTTGATCTTTACGAATTTTTTTAAATCCATTAATTTCTTTTTTAAATTCTTTAGTTATGTTTCCCTTTTTAAGGGTGTATTTAACTATTAGTGTACTTTCATCATCTACTTTAGGACTAATTTCTTTAACGCTAAAAACAACACCAGACGGCAAATTTAAAGGCTTTATTTCTTCCTTTATTGCTGCTGATGTTTCTATATCTTTGTTTTGGGGATATTCTAATTGTAAATTACTATTTATAGTAGTTAATTCATCTTGTTTTTGATCTACTTCTTTTTCCTTTTTTGTTGTACTACATGCTGTAGCCATGATCGCGACAATAGGAATAGGTGATAAACTTAAAGTAATTAATTTCAACTTTTTCATAAATTTTTCCTAACTAATAATGCTTTTTGCATCATTGAATATGAAGTGTAAATACTCAAATTTATAGTCAACTTGAATGTTTTTTAATTATAAAGCAAAAAAAAAAAAAAAAGAACAATTTTTTAAAAAAATAAATTGGTTTTTACAAAAATAAAATAAATATTTATGTAAATTTATTGTGTAAATTATTTGTTTTTTATCAATACTTTTTTCTTTAATTTTTGAGCATAAGCGAAAGCAATATATTCATTCTCATTCATTTTATGGATTTTAGCTTTATAAACATTATTGATTTTTATAAAAGCAATAATTAAATTTAATAACATATTAATGCCATGACATATTAAAACAGGAAGTGTAAGCGCGTATAAAGATAAGCCATAATCCTGATTATGAATATAAATGATAATTGTTAGAATAGCACTTAAACTAAAACATAAACGTGCAATAAAACAAATAATAAACATACTAATTGTTAATGCAGCGGTGTTTTTAGTTTTAATAGTTAAAAATGTTTGTGGTAAGAAAGCAAACAATAAAATTAGGCTAGCACTTAATCCAATACTTATTTGAATTATTAATTCAATACTAATTGGCGACATTATAAATTATTTAAGACATATTTTTCATAATATGTCTTTTCATCTATTAAATATAATTTAGCTTTTTTAATGTTTTTAACTTTATAAATTAAAATAACTAGACCTAAAATTCCTACTATGGTGTTTGCAAGACACAATGGTAAAGCAGTAGGAATACCATTGTGTCTGTTAAAAATCATTACTAATATACTGTATATTACAAATAGAAAACAAGCAATGTTTAAAGAAATTAAAAATGTTAATGAGATTCCGTCTGTTCGTTTTGTTTTGATAACTTTAATTACTTGGGGAATATATCCTATTACTAGAAAAATAGACCCAAAAATGCCAATTACTAAAATAATAACTGGTAATGAACTCATAATATAACTCCTTTATAAAAAATTAGAGTGGTTTTTTAAGAATTAGATTTCAAGGACGAGGAAATTGCAATGGAGTGATTTGTTTTTTTAAGTAAATACCAACTAAATGATGATAATCATTTTCTCAAAAATCTTTTAAAGCAATTCTTTCTAAATACAAAGTTTTTATAATATTTTTTGCTTTTAGTTCTTCTTGATAAAATTTTGTTGATTTTGGTTGAATTAAATAACCATTAACTTTAACAAATGCGGCTGAAATTTCTAAAATTTTATCTAATGACGCAACAGCTCTTGAAGTTGCGACATCAAAAGATTCACGCATCGATTGTGTTAAATCTTCAGCACGCATATTTCAAATTAAAACATCATTTAAACCTAATTCTTGAGTTAATATTTTAAGAAATTCACATCGTTTTTGATTAGCTTCTAATAATGTTAGATTTAAATTTTTAAAAATAATCTTTAAAACAACCCCTGGAATTCCAGAACCAGAACCAATATCAATTAATTTAAGATTAGTGTTTTGTGTAAAAAAATCTAGTTCTTTATAAGGCGCAAGTGAATCTAAAAAGAAATTTTGGTAAATCTTATCATCACTATCAAGTCTTGTCAAATTAAAAATTTGATTATATTTTTGAATAATTGTTTTATACTTTTCAAAAAGTAAAAAAGTTTGTTCATCAACTCAAGGAAAATATTTAGTTAAAACATTAAAAAAATCTTTTTTAGTCATTTTTAATTCTTTCTAAATAATATTTAATCATTGCAATGTCTGTTAAATTAACCCCACTAATCCGACTTGCTTGTTCAAGATCAAGAGGTTTAATCTTATTTAATTTATCAATTGTTTCTAAAGAAATATTCGGTACTTCTTTATAATCAACAATTGTATGAAGTTTAATATTATTTAAACGCTTTAATTGTTTTAAGTTTTCTTCTTGATTTTTAATATAACCTTCAAATTTAACTTGAATTTGTACCTTATTAATAATTTGCTCATCTTCAATTTTTATAGGACAATAAATTAATAAATCATTTAATTTAATTTCAGGACGTTTTAAATAATCAATTAAATAACTATTTGTCTTTAGAGTTGTAAATTTTAAGTCATCAATTTGGCCAACAGTTGTTGTTTTTAATCAGTTTAAGATTTCTTTAATTTGTTTTAAATTATTTAAATATTGGTCATATACTTCAGATTTTAATAAACCAATTTCAAAACCAATTTTCATTAATCGATCATCAGCATTATCATTTCTTAATGCTAACCGGTGTTCAGCACGGCTTGTTAGTAAACGATATGGTTCGACAACGCCCTTAGTAACAATATCATCAATCATAACACCAATATATGCTTGATCACGACCTAAAATAACAGGTTCTTTATTTTGATATTTTTGATTAACATTAATTGCTGCCATCAATCCTTGTGCTGCGGCTTCTTCATATCCACTAGTTCCATTGATTTGACCAGCAAAAAATAAATTTTTTACTAATTTCGATTCTAAACTAGGATATAGTTGGGTCGGATCAATAGCATCATATTCAATAGCATAAGCGTATTTTAAAATTTTACAACTTTCTAATCCTGGTAAACTACGGATCATTTTTTCTTGAACATCGATTGGCATTGAAGTAGAAAAACCCCCTAAGTACATTGAATCTAATTCATAAGATTCTGGTTCAACAAAAATTTGGTGACGCGGTTTTTCACTAAATTTAACAATTTTATCTTCAATTGAAGGGCAATAACGGGGCCCAATTCCAGAAATCATACCCCCATACATTGCAGATTTATTTAAATTTTCTCGAATAATTTGGTGAGTTTGTTCGTTTGTATAAATAATATGACAAGGAAGTTGTTCGTCATAAGGTTTATAAACAGGGTGATAGTGACTAAAAGCAATTTTTTGATTTGTGCCAGGTTCAAGAATCATGTTTGTAAAATCAATCGAATCTTTTTTAATTCTTGCAGGAGTCCCTGTTTTTAAACGAATTAACTTAAATCCTAATTTTACTAATGCATCACTTAAAAATTTTGCATTTTTTGTACCATCTGCTCCTTCATCAACACAAACTGAACCTCGATGAGTAATTGATTTTAAATAAGTTCCTGTTGTAATAATCACATAATTAGCATAAATGATTTTTTGATCACTTAAAATAACACCTTTAATAATGTTATTTTCTACTAATAGATCACTAACTTCAGCAATAATTAAATCCAAATTCTTTTGTTGTTTAATTTGTTGTTTAAATCATCGTTGGTAAGCAATTTTATCAATTTGTGCACGAATCGCTCAAACACCAGGTCCTTTTGATGAATTTAAGATTTTCATTTGCATTGTTGTAGCATCAGCTGCCTTACCTTGAATACCACCAAGGGCATCAATTTCACGTGTAACAATTCCTTTAGCCGGTCCTCCTATTGAAGGATTGCATGGCATCATACCAATCCCTTTTTCATCTAATGTAATTAATGCTGTTTGTAAATTAAGATTAGAAGTTGCAAAAGCTGCTTCTAATCCTGCGTGTCCAGCACCAATGACAATTACATCATATTTTTTCACGTTTAACCACCTAAACTATTACTACTTAAAAATTATAACAAGATTAAAGAAGAGAAATTTTTTAACAACTTAATTTATTGATTAGTTTTAATTTTTCATTCACCATTTTTTGATGATCCCACAAATTCAACATATCCATCATGTACTAATTTAGCTAGATCACGTTCAATAGTATTAATTGAAACAAAAAATACTTTTGTTAATTCATAAGCTGTTGTTTTTGGATTTTGTTCGATAAATTCCACGATTTTTTGTCGTCTTTGGCTAGGGGATATTTTTTGATTTTCATTTTTTGATATATTTTGCTCTTGATAATTTAAATTTTTTAAAACAACAAAGAAAGCAGATTCGGTTGAATGAAACTCCGGTTTTAAATCTTCTCGATAATTTTCTTTAGATGCATAAATTTCTAAAATATTTTTTAAACCAGAACCTCTTCTTTCCATTAAATTCATTCGTGCAAATAAATCAGCTAAAATAGGATTACGGCGAATTGATCATACATTATAAGGGTCAATATTTTGGACAAAATTCCCATCAAACATACCACCAGGAGAATATACTTCTAATCTATCATCATACATGTCAATATGGACTTCACTACCAATAATTGAATAATCTCGATGAATTAAAGCATTAACTATTACTTCTTGCACTGCTTTTATAGGATATTCAGGATATTGAATACGATATAAAACTCCTTTTTTTCACATGTTTTTACTATTTCTTTTAATAAAATTTATACTTGAATTTAAAAGAAATAGTAAATTCCCTTCAAATTCTGCATCATCTAATGCTTAAATTAAACCATTTGTTTTAGTCTTTCCATTTCAACGAGTACAAAAAACTCGTGATTGATAAACTTGATATCCATCAGCAAATAAAGCTCCAGCATTTGTTAAGTAATTATTGTTATCAATTAATTCAAAGGATTTTAAAAATTTATCCTCCCATAAATTTTCTGTATGTTTTTGATATTCATTTTTTAACATCAAAAAACTAACATCTTTTAGTAATTTATTCGAAACTAATGAATCATAGGTGCAATTTTGACTTCTTAAACATCAATTTAATAAATCATTACGTGAAGCAATTACACTTTGATTACCAACTCTTTTATATGGAGTTTTTGAACCATTATCAACTAGGAAATATGGTGGATTTCTTCCTGGGAAAATTGTTAGTATTAGAATAATTTTGCTTTCATATTCATTAATTTCAATATCAAATTCTGGAATATTATCAATTTTTGTTTTAATAGTTTCACTAATAAATTCAGAATCATTTAAATAATTTTCTAATCCTAAAACATCATTGTTTTCATTTACACCAAATATTAATTTGCCACCTTGACCATTAGCAAACGCACAAACACTTTTTAGTCATGATTTTATTTTCTTCTTTTCTAATTTTTCTTTCTTATCATAAAAATTAGTTTCACCTAAATATTGTTTTAAATTCAACACCAAAATCTCCTTGTATTATTTACAAGTCATTATACTACTAATATTGTTATTAAAATTTTGGATAAATACTAAATTATTGTGACTAATAAAATTTAAACTATTAGTTTTTTAGTAATTTTTAATAAAGAGTCATTTAAAATTTTTTCAATTTTATTAGCTTTTGTAGACAAATTTAGTAGTGGTTCGACAATTTTAGAAAATTCTTCTTGAATTTCCATATTAGGTAGATTAATTTTAATTTCTTTTAATGAATATTCTCGTACAGCAGGTCTAGAAGAACCAACTTGTGATTTAAGTCTATTTATTTCTTGTTCTTTCTTAAGAATGTAGTAAACAAATTTATTATTAAATTTAAAATCAATATATTTATTTTTTATTAGTATAAAACAAACGTTAGTTATAGAAAATTTTCCATTTTGTAAAAATACAGTTCCAGCATAAGCTCCATCAGCACTTATTGTAATAAATTCGCCATCATACATATAAGAATTAATATAGCCAAAAATTCCATTATTTTTTGTGTTTGATGAAATGACGGGATATGGTCCTATATTGTTATCTATATATAAGGATTATTTAATAAACAATATAATAGTCCAGAATTAGATAAAACACGCTTAGGAAATGTGGTACGTAAATTTAATGATTATGATTTTTCACAATTCAATGAGGATTTAGTAGGAAGAATTTATGAATATTTTTTAGGCGAATTCTTTCGTAAACAAGGGCAAAAAGGTGGAGAATTTTATACACCAAAAACAGTTGTAGAATTATTAATTGATATTTTGGATCCAAACGATAATATTAAAATGTACGATCCTGCTTGTGGAACAGGTGGTATGTTTGTTCAAGCGCGTAATTACTTACATGAACAAAACAAAGATTATAATAAATTAGTTATTTATGGCCAAGAATACCAATCACAAACTTGAAAATTAGCTAAAATTAACCTTTTATTAAATGGTTTTAATGAAAATGATATTCATTTAGGACGTGGATCAGAAGATACTTTTAAAGAAGATTTACATAAAGGTCAAAAGTTTGATATCATTGTTGCCAACCCTCCTTTCAACTTAAAAAAATGATATCGTGAAGAATTATTAAATGATGAACGTTTTAGTTGAGGAATGCCACCAGAAAATAATGCAAACTATGCTTGATTATTACATATTATTTCTAAATTAAATTCACGTGGTAAAGCTGGTGTAATTTTAGCTAATGGTTCATTATCTTCATCTAATAAAGAAGAATCATTATTACGTAAGAAAATGATTGAAGAAAATATTGTTGACGCAATTATTAGTTTGCCAGATAAATTATTTTATACAACACAAATTTCTGCATCAATTTGATTCTTTAATAAAAATAAAGAAAACGAAAATGTTTTATTTATTGAAGCAAGCAAAATGGGCGAGTTAAAAACTAAAAAATTACGTTTTTTAACTAAAGATGACATTTTAAAAATTAAGAATGTTTATGATCAACATGAACAAGGTGAAGACGTCAATGTTGTAGGATTTGCTAAAACTTGTACAATTGATGAAATTATTGAAAATGATTATTCACTAGTTCCTGGACGTTATGTTGGTTTTGAACAAGAAGTGATTGACCATGAACAATTAAATCACGAAATTAAAGAATTAAAAGAAGAGTTATCAGTTTTATTTGATGAACTTGAAGATTTAATTCCTCAGGCCAAAGAGTCAATTAAAAAAGTATTAGAAAAATAAATTTTAAAATTGTCTTTTGTAAAATAATAGGCAACTTTTTAATTCTTAATTTATCAAAATTAAAAGTACATTAAAATGTCTTATTTTTTAAATTATGTTAATATCTAAATATAATATTTATAAGGAAAGAGGTGCATGAAATGAAAGAAATAACACCATTAATTGTTGCTAATTGATTTTTAACAAAAGAATCAATGACTCCCAAAAAAGTTCAAAAATTAGTGTATTATGCATATTCATGATACCTAACTTTAATGAATGAAAAAGTTGATGATTTAAAAAATAAATTATTTGATGAAAAAATCAAAGCTTGAGTTCATGGACCAGCTATTCCTATGCTTTATAATGAATTCAAAGAACATAAATATAATAGTATTCCTAAAATAAATGATTTTAATGAACTAGAATATTTTAATTTAGATACTATTGATATATTGAATCAAGTTTGAGATGAATATGGTCATTATAGTGCTAATCAATTAGAAAGTATTACACATCAAGAAGACCCATGAATTAAAGCTCGCAAAGGGTTTGGACCACTAGATAGTTGTAATGCAGTAATAAGCGATAAAGAAATATTTAGTTATTATATAAAACAAATGAAGAATAATTAAATTATTAATGTCTAAGAAAATAAATAACAATAAAACTCCTCGTAATAAAGTTAAAAATAACAATGTTTCAAAAGATAATGATGACATTGTTATTTCTTTGCGTTATGAGAATTGAGTTAAAGGGGGACGAGTAAATAGTTTAACGACTTATACTAAGGATGAAAACCAATTTTTAAAAAATATTATTTATATTCTTAACACATTGTTTCCTTATGTTTATAAAAATTGAAAAAATAAAGGAATTAACGATCATTGCCATCTTATTAAACAAAATAATGTATTTAGTAAATATATTAAATTAATTAAAAAACTCCATCCTAATTTATTAAAAAATGATAATGAAGAATTAGAACTTTATCAACTAGGCCTTGGAAGATCAGTTAGAGTAATATGCTCAAAAATCGATAATATTTTATTTCCTTTACTTATTGATCATCATCATTTAGGATATGAAAGTCAATCTTATAATGATAAAGATACTAAAAAATATGATTATTGTCCTTTAAGAAAATATAATAAAAATCAAGATAACTAATTATTAATCTACTTTGTTTTGTAAAGTAGATTTTTAAATATTATCACTACTTTTAACTTAACTTAAAAAGATACTTAGACTCAAAATGATTTTGATAACTATTTGAACTAATAGTAATTGATTTTCAACTAACTTTTTTAATTAAGATTATTAAAATGATAAATGAACAAAAATAAGGTAAATAAGCAATAAAGTTATTTTCATAAATAGTGTTTTTTAGATTGTAATTAAAACTACTTATTCACTGAAAATTATCATTAATAATTTCTGGATATAATAATGCACAACTAAAAGTTGTTCAATAAGTTAACATACCATTATAAAAGTTTGTATGTTCTAAAAAAAGTAAATGAAGTTCCAATAATCATTAAGATAAAATTAATAAATAAATATAATGATAAAACTTTTATCATGAACAAATTTTGCTATAAATATTGTTAATAAAATATAAAATCCACAACGTAATAAATAAATGTAACTATATTCTAAAATTTTAATTTCATTAATTCTTACTGGTATAAGTAAATTATGAATACGTTTAATGTAATAGTAATTTTGATTAATAAAAACAATCATTAAATTAATAAATAGTGTTAATAAAAGTAGTGTAACAATACTAGAGATTACAATAAGAATCGCATTATTTTTAATCGATTTTATTTCATTAAAATTTTTATATTGTACTAATTTTTTACGAAAACGATAAATACTTATGCAAAAAATATAAGATAGTATAATTGCTAAAATTTTATTAATTAATGAAAACCGCAAAAAGAAAGCTAAAGCGATTGGTGAGTGATATCATTCTGATGCATTTCCATTTATTTTGGCAACAATAGCCATGATAATCAAATATAAACAAAAACAAGTTAATAAAATAAAAGTAAACAAAAAAGTTTTAATTAATTGTCATAGAACATTATTATGGGTAAATGTTTCCCTTTTGTAATGAATTGTAGATTTCTGTCATTTCATTTCCATCGTAGTATTGTCCTTTATAATTAAAAACTTTTTAAATTCGGTTTAACATTTTGCTTTGGTTTTAAGGTATAAAATAATTTTAATATAAAGATGATTAGGTAATGCATGTATTCGCATAAACTTACAACTTCAGAACCACTATTTTCGTAACCCACTTTTATAATTATTTCTAGAAGCTCAACATCATGATTCTTTTAATAAATTACTAATATTTGGTATAAATGAAAGAACATCCTCTTTTACAAATTGTTTTAAATTAAGAACTAACTTTAGGGTTTAGTTTATAAGTGTAAATATTTTGAGCTTTTAGTTTTTTTATCACCGCAGCTCTTACATCAATATCACCACTTAAGTCTTGGGATTGAGCAACAAATAAATTACCGATCATTGAATCATTTGGCGCACCATAAGCACGCGAATTTAAAGAAGCAAAATGAATACCTACTAAATTAAAGTTAGAATCAATAATCATTGAACCAGATGAACCAGAACCCAAATCACCTATTTCATCAGAGGTAATAACTCGTGTTCCAACATTAAATAATTTATTTAAATAACCTTCACCAACCTTTTGATAACCATCAATATTATTAATTTGCTTTGGACCATAATAATCTAAAATTGATTCATTTTTAATTTCACGATCAAATGCTTGAATTAGTGAACCATAAGATTTAGAACCGCGCCATGAAACAATCGCTGAACTATTAGGATTAACATCACCAGGATATCCTCCACTAAATAGTTGTAATTGTGGCAAGTATTTTTTTGAATTGCCTAATCCAATATATCAATTTGCTTCATCTTTTGTATTTAATACTTTATAATAATTTGGTAATTTTTCTTTAATATCTTCAATATCAAAAACCATTGGGACCAAAACAAAGTCTAAAGTTCCATTTTTAATTGATTTAATATCTTCTTGATTAATTTGATATCATGTTCTTAAATTTTCATTAATTTTTATACCATTAGCACTTAATTGTGGTATATATCATGCTCGATTAATGAAATTTTGATTTGTAATATTATAAACCTTTGCTAAATTTGAAAAACTAATACCACTTAATGAATTATATTTATTACTTACTGGAGATTTTAGAGGCGCAGTCCCCCATAGTAAATATTGATAACGGTCTTTATCTTCATAAATGTTTGAGGATATTAAACCAGCGTTATTTTCTAAATAATTTATATATTCTTGTTTATTAAACCAGCGTGATTTCATTCTGTTATCTTTTGAGATTGCTAAATTCGATCAGCCCATAACATGATTATTAGTTGCAATAAGTAATTTAATTTTATTTTTAGGTAAGCTATCGTCAATAATACGATCAACAATTCAACCAGTTCCAGAATTTAATTCAACATTTTTTCCATCTTTTGTTGTTAGTAAATCAAGTGAGATGGTACGATCAAAAATATATTGAAAATAATCTTCATTTTTTAATAATCCTGAATTCTTTTGATTAAAATTAGCACTTAAATATACGGTTTGTTTTGTTTTTTTGTTATAAATTTCTAAAATAAATTCTAATTTTGCATAACGATCAATAATCCGATAATAAATATCTTTAACAACAATATTGTCAACAGATTCGTTTTTAGTAATTGTCAAGAATTTAGGATATTCTTCTTTTTTAATTAAATTAATTAGTTGAGCGAAATTTGTGGGGAGTTTACCAAAAGGATTAATTTGGATTAATTTTTTGATTAATTCTATATTAATACTATAATTGTTGTTTGTTTTTTTATCAATATAAGATGTAAGCGTTTTTGTTTCTTTAGCTAAAATTTGCGGAGAATTAATTTGATAAATATTTTGCTCATCATAACGAATTTTAAAGGCGTTTTCTAATCTTTTTTGGACATCACTACCAAATTCAGAATAACCGATTTTGTTCATATAAAGTTTAGAATCATCAACATTAGCTTCATTTTGATTAAAATAAATTTTTGCACGCAATAATTGTTCTCTATAGTATGGATTATTGTGAATTAAATTATAAGGGTTGTTTGAGAATTTTAAGATTTCATCAATACTATAAACAGCAGGTGTTTTATATTTTAATTCTTGTAGATAATTGTTTTGTTTATCTGTTAAAAAAACAGGCTTAGTATTAATTAAATAACTTTGTTTAGTGGTTTTAATGTAAATGTTAATACCTAACGTTCCATCATTATCATTAGCAGCTCAATTAAGAATTTCATAATCATTTTTATCTAAAATTTTTTCTTCAATTTTTTGATTTGTTTTAATTAAATTAAAAATAAAAATAAAATAAGAATTAATTTGTTCTTTGTTGATATTTGATGCTAACATTTGTTTTGTTTTATAAGGATTAATATTAACAACTTCAACATTTCCTTTCAAGTTTGTTTTAGCATTGTCAAGTTCATTAGAATTTATATGTGCACAATTAGCTAAAATAGTTGGAATTATAACAATTGGAGCAATAACACTTATAACTTTTAAAAAAAATTTTGTTTTTAGATTCATAAAATTTCTCCAATTATCTTTTACTTAGTAATTATAAAGCATTTAAATTAACATAACTAATTTTGACTATCAAAAGTTTATAATTTATTAATTCTAAAAGGGGTCCAAAATGATTGATTTAATTATTAAAACACTAGTTCAAAAATTAAAAATTGAGGCAAAATATATAACAAATGTTTTGAATTTATTAGCTGATAATAATACTATTGCATTCATTGCTAGATATCGTAAACATTTGACTAACAATATGGATGAATTTCAAATTCAAGCAATTGCACATGAATATGAATATCTTACAAAATTAAATAAACGTAAAGAAGTAATTATTAAAAATTTAGAACAAAAAGGTTTATTAACTAATGACTTATTAGAATTAATTAATAATTGTCAACGGCTTGTTGATCTTGAAAATCTTTATGAACCTTATGCTTCAAATAAAAAAACAAAAGCATCAATCGCCATTGAAAAAGGATTAGAACCATTAGCATTATTAATATTAAAAAATAATCCTAATTCTAATATTAAAGAAGTTGCAAAACAATATTTAAATGAACAAGTTTTAAGTGTTGATGAAGCTATTGCTGGAGCTTGTGATATTATTGCCCAAAAAGTTGCTAACGATACAACTTTAAGAGAAATGCTTTATGAAACAATTAACAAACATGCTAAATTAATCACAAGAATTAATAAAATAACAAATGACCCAACTAAAAACTTTGCTTTATATTATGAGTTTAGTTGCCCAATTAAATATTTAAAAGCATATCAATTAATGGCTATTGATCGAGCTAATGAATTAAAAATCATTGCATTTAAACTTGATTTTAAAAAAGAATTCCTTATTGATTTTGCTATCAATAAATATACTAGAAAAATCAAAAGTAACAGCTATGACTACATTAAATCATCTGTTAATGATGGTTTTGATCGTTTATTAATTCCTTCAGTTTCAAATGCTGTTTACAAAGAAAAATTAGAGGAAGCTCACCAACAATCAGCACAAATTTTTAGTAATAATTTACAACAATTATTATTACAAAAACCTTTAAAAGGCCATGTGGTTTTAGGATTTGATCCAGGATATGTGCATGGTTGCAAATTAGCAATTGTTAATAAAAACAATCAATTATTACATACTGATATTATTTACCCTCACAAACCACAAATATTAATTACACAAGCTAAAAATACGTTAATATCCTTAATTAATAAATACGAAGTTAATACAATAGTAATTGGCAATGGAACAGCATCAAATGAAAGTGTAATTTTTATTAGTGATTTAATTAAGGAATTCAAATTAAATCTTAATTATTGTGTAATTAGTGAAGATGGTGCTTCAATTTATTCAGCATCATCAATTGCAAGTGAAGAATTTCCTAACTTAAGTGTTGAAAAACGTTCGGCAATTAGTATTGCACGAAGAATCATTGATCCTTTAAGTGAATTAATAAAAATCGATCCAAAAAGTATTGGCGTAGGTCAATATCAACACGATATTAATAAAAACATTCTTCAACAAAAAGTTGATTTTTGTATTGATTATTGTGTTAATCAAGTTGGTGTTGATGTTAATACTGCTTCAATTCCTTTGTTATCTAAAGTATCAGGTTTAAATAAGCGAAGTGCAAAAAAAATTTTTGAATATGTTAAAAAACATCAATTTATTGAAAATCGTGAACAATTAAAAACCATTCCTTACATTACCGATAAAGTTTTTGAGCAAGCCGCTGGTTTTTTACGAATTAATAATGCTAATAATTTTTTAGATAGAACAAGTATTCATCCAGAAGCATATCCAGTTGTTAATGAATTATGTAAATATTTACAATTACCAATTAATAAATTAATAAATAACTATCAAGTTTTAAATCAATTAAATCCTAATGAATTAACTACTATTCTAAAAACAGATATTTATACTATTGAAAACATTATTAATAACTTAAAAAACCCTTTACAAGATGTTCGTGATGATTATGATATTCCTATTTTACGTTCACAAATGGTTGATATTAATAATTTGAAAATAGGGATGTTAGTACAAGGAACAATTCGTAACCAAACAGAGTTTGGTAGTTTTATTGATATTGGTTTAAAAAATGATGCATTGTTACATATTTCAAACTATTGTGAAGAAGATAATTTGCATGTTAATAAAAATATTAATGTTTATATTGATAAAATAGATACAATTACTCAAAAAATTTCATTAAAGTTAAAATTATAGAAATTATGAAAATAAAATTAAAAAAAATAATCATGTCTTTTTTGGGATTGCCAATTTTGGCAATTCCCCTTATCGTTAGCGCTTGTTCGCAATTTAAAATTAACCAGGATGTTATTTTAACTTATCGTCCAACAGTTTCATTAGCAAAAGAATTTGCTAATGATAACAATACTATTGATGATGTTTTAAAAAAAGTAAAAATCAATAAAGACGGATCAAATAACCTACTAGTTTATGATTTATCAAGAAATCCAAAGAATGTCCAGTATAAAATTGTTGATATTAAAAAGGATTCAGAACAAATTCTTAAAGTTACGATTAATGCTAAAATTCAAAAATATAAAGAATCAATTAATTATGATTTTTATTTTCAACCTTTTTTAACTCCTAAACAAAAAGAAGATAAAACAATCCTTCAACAAAATTTAAATATTATTAAAAGTGCTTGGGATTATGATCAAAAACAAAAAATAGGTTTTTTTAATCTTAGAATAAAACCAGAATATCAAAAAAGAAGTTTGGTTGAAATTAAAACATTAGGTGAAAAAGCTTTTAATTTTGGTGAGGATTTAATTCCTCAATTAAAAGTAGACTCAAAATTTAAAGATATTAATATTAAAATAATAGACATTAACTATTTTGAACCATTAGATGAAAGACAACGTGAATTAGTTGTGGAAATAATGATTAGTAAAGGTAAAAATGAACAACAGGCTCAATTATTTAAAAAAATTAAAATTAATTTAGACTAAAGGGTAAAGAAACAGTGAATAAAAAAATTAAAACAGTTTTCATTAGTAGTTTAATTGTTGGACTATTAGGTTTGATAACAACAAGTGGTGTTGCGTGCAGCTCATCAATAAAATCAAAACAAGAGAATGAAATTTATCCAACCATTAAAGGTTATGATCAACATGATTTTTCAAAGATTTTAGCTTCAGAATTTATTAAGATTATTAAAACTAATATTCAATTTTTGGCACCAAATAAAGATATTAAATATGAATTATATGATGCACGATTGAATGAGTTAGATGAAGATGTAATTGATCTAATAATTCGTTATCATTTTAAAGGTCAAACGCACACTTACAAAAAAATCTTAAAAGGTTTTAAGAATGCTAAACAATATCGTTTATTTTTAGAAAATAAAAAATATTTAGAAGATGAAAAAAACCATAATACTCTTTATTTAATGCCCATAGTTAATTCAAGTATTAACATTAGTAAAACAACAGTAGATGAAGCATTAAAATGACAAAATAGTTATTTAAATATTAACAATAGTAATAATGATTTAAAATATGAAATTCTAAAAATTACTAAAAGTAAAACTGCTAATTCTATTGATATCGAAATTAAAATCAGTAAAGGTAATGGGGAAAATTTAGCAAGCATTACTTATAACAAAACACTTAATGGTTTTATTTCTGAAGCTGATAAAAAGATTTATTTAGCTAATTTAGAAGCGATTAAACAAGATAAAAATCAACTTGAACCACAATTAATTGATGAAAAAACAAAACAAAAAACAATAAAAGAAGTTATTGAAAATTATAAAACATTATTTAAATTACCATTCCAAACAGGTTTTATTTATGAAATTCAAAGTGTTGAACCTGATGTTAATAACAAAACAGCGGTGGTTTTTAAGATTTTGATTAAAAAAGGCAATGGAAGTCAAGAAGCAGTTTTAGAATATTTAAAAACGATTAAAGGATTTAATCAAAATAAATAATTTATTTAAAATAATATATTTTTGTTAAAATTTATAAGTTATTTTATTTTTTTGATTTAAATAACAAACAAATATTTAAAATTACGAAAGGTGGATTCCATGTCTCAAATGGAAAATTCGACGAAAAAAGTTGAATCGGTAGCTAACGTTGAAGTAGTTACTACTGAAAATGCAAAAGTAGAACAAAAACCTACATCACCGGCTGATGCAATTCAGCTTAAAGTCAATGCAAATTCAACTATAAAAAATCTTAAAACTTTAGTTTCAATTGTAAAATTAACTGAATCAGGTGCTCATATTGGGTTAAATCCTAAGAAATGAAATCCTAAAATGGCTAGTTACATCCATGCTAAACGTTCAAATAACCATGTAATTGATATTTTAAAAACAATTCTATTTTTAGATCGTGCATACAAATTCTTACAAGAGGTTGCTCAAAACGGGGGCACTGTGATGTTTGTGGGTACAAGAGGTCGTGTTGTTAAAGAATTAATTAAAGCAGAAGCAGAACGTACTAATTCATTTTATGTAACTCAAAGATGATTAGGTGGAACTTTAACAAACTTTACTAACATCTCACGTTCATTAAAAAAATTTAATAGTAATTTAGCTTTATTAGAAAGTGAAGAGATTAATAAATATTCTAAAAAAGAACAAATTGCAATTAATAAAGAAACTGCTAAATTAGAAAAATTCTATGGCGGGATTAAAAATATGAAACAACGTCCAGATGTTTTAATTCTAGTAGATCCTGTTAATGATGTTAATGCTATTAAAGAAGCACGTAAATTAAACATTCCAGTAATTGCTTTAGCAAATACGAATGCTGATCCACAATTGATTGATTACATTATTCCAGTAAATAATTATTCAGTTAAATCAATTACTTTGATTTTAGGTGTGCTAGCAGATTCAATTGCTGAATTGCACGGTGAACCAACAAAAATTGTTGGACGACCAGATAGCGAAATTGTTTTACCTGAAACAAAATCAAATTGAAAACAAAATAATTATGACCCAAGCAAACGTGGTTATAATCCAAAATATGTAAATCATAAATCTACATTCAATAAATTTAATAACAAAAAACCAGTTGATTCAACAACCAATGAAATAAAAACAAACGTTATTAAAGCAGAAACAAAATAATTTAATGTTTTAAGAGGAAAAACACAACCGATGCGGTTGTGTTTTTATTCCTATTATTTTAAAAATAAGATAATTTATATACAAAATATAATGGGAGTCGCTTAAAAAAATATCTAATTTGATATATTTAATAGATATTAAAAATTAAAAATAAAATATTCAATTAATGCAAAATTATACCTTTCGTTTTAAACTATTTAAACAAATTAAAAATCAACAAGTTAAAAAATTAAAGCCACTCATTTTAATAGGAGCAACAGTTTTTTTAATTATTGCTATTTTTAGTATTTGAGTATGAAATATTTCATATGCTAATTTTTATGATCAACAATTAAATAAATATGTTTACTTTCCCTTACACGAGTCCCTTAAATTAATTTTTAATGGACATTTTTTAGATCCAACAAATATTAAATATTTAAATTTACAAGCTACATTTTGAGCAAGCATTTCTTCAATTTTATCAGGTATTAGTTTGGCAATTGCTGGATGCGTGACACAAGCACTAACTAAAAATCCATTAGCTGATTCAAGTACATTAGGTATTGTACAATCAAGTGTATTTATGATTGTAATTGCATTCTCATATAACATGATTTCTTTTGGAGAACTTTTTGGTTTTGCCATTATTGGTGGAATAATTGCTAGCATTATTTTATTGATTCTAGTTTTTGCAACAAGAAGCAGATTATCATATATTAAAATTACTTTAGCAGGATTAGCTATAGGAGTTTTTTTTAATACAATTTCTTATTTTATTCGTATTCAAAGTGCTAAAGGTTCAAGTATTAATTTTAAATATGTTTTAGGGGGTAGTGAAAATATTTATACAACTCATATTGATCCTTTTTTAACATTATGAGTTAGTGCAATTTTAATTCTAATTGGTGTAATATTAGCTTGTTTTCTATCTTACAAATTAACCTTATTAGAAATTGGTGATGAAAAAGCTAAAAACTTAGGATCGTCAATTATTATTATTAAAATTTTAGCTATTATTTCAACTATTTTTTTAGTTTCACCAACTATTTTATTAGTTGGTAATATTGCATTTGTTGGATTATTTGGTCCCCATATCGTGCGTAAAATTTTTTCAATTAGAGATTATCGTTATGTTATGCCCTTATCAGGATTATTAGGTGCAATGATAACATCTTTAGGTTTAATACTATATCGTGAGACAATTTATATTAATTCATCGATTTGAATGAGTTTTATAGGAGCACCTGTTTTAGCTTATTTGGGTTGAAAACATTGAAATAGAGCTTAATTTTTATTAAAATAAATAATAAAATGGTTTTAATAATGAAAATTAGTTTTAAATTTAGATTAATTAATGTAATTTCAATGCTTGGATTAAGTATTATTTTTACAACATTAAGTTCATGTAAAAATCTTCAACAACAAAAACCGTATGATACTAATTGATTATTCAAAAATAAAAATTGGAGATTGAATAATTTTTTTCGTGATCCTTATAAAATTCATTTAGAATTTGCTCGCCAATTAGATAATGTTTTAAATTTTAATGAATTAAAAAAACGTCTACCAAAACCAATGCAAAATGTACATTCATTTACTGAATGAAGTCTAAAGATCGCACGTTTATTAAAAAATGATATTAATAATGTAGCAATGCTCGATAGCATCCAAAAAATGCGCAATCATAAAGCAACTGTTTTAGTTATAGAAAATCGTAATGAATTAGTTAATAATAAAGAAAATTATAATAAATTTTCTATTCTAACACCTTCAGATTTTCCACTAATCTATTCTAATCCTTATACAGAAGTTCCAGGATTGGGATTGAATTTTCCAAAACCAATTAATAATGATATTAGTGATATTATTGATCGTTATCAATCAATTGGTTATAATGCATTAAGAACAAACCCGCTCGAATCAGTCTTAAAACAAACATCTTCATTTTATAAAACAGCTGATTATGTTTTTTATATGTATGATTCTAATTTATTTACTAATAATAAATACAAAACAGATGGTCAAAAACGTGAAGATTTATTTAAAGAAATTTTATCAAACAAAAATTTTTATGCTCATAAATTCTTACGCAATTCTCGTTCAAAAATTATTTTTATTGATCGTTCATTGTTTTGATGAGGTTCTTTTACAATGGTTGGGCAATCACTAATTATTAAAGAATTATTAAAAATTTTCACAAATAAAAAAACAATAAAAAAACAATGAAGTTATCTCCCATTTAATAAAGAAGAATTAATAAATTTATTTGATCACAAACCAACTATTAAAGAGCGTAATTTAATAATTAAACCTCATTATATAAATAAAAATTTTAAGCTAACTTATGGTTATAAAATTGTTGGGACATGAGCAGATAGTATTGATCATTTAATTTCATTCGGTTTAAAACCTGATGCAATAGTTGATTATGAAAGTTTTAATAATATTAATTTAAAAACCCAAGGTGTTGGTAATTATTTAAAAGAGTTGATTAATTATGATAAATTAGAAAAAATTAATAAGGCTCCTAATGATAGTGAAAAAGCGGAATTTGTTGATAAAAATTTTGCAATTTATATGGGCCCATCCCATTTATTAAACTCAATGAATAAATTAATTATGATTAAAAATACAGGATCTTGAATAATTAGTAGTACACAAGGAATTAATGATCCCAAAACCCAATTTAAAACAAGAATCAACACGGCTAAATCAGATTTTTAATTTATAATAATATATTATGAAAATAAAATTGAAATTTGAAAATAAACTTAATTTTAAAAAATATTTAATATATAAATGAAGTGTTTTTGTTGTTTTGGTTTTGTTAACTTCTTTTATTTTTATATTTAACATTATTTATACTAACAAAACATTTATTTGAGAATCGTTTTACTTTCAAAATGCTTCATTTGAAGAGGTTTTTTTATTGTTTTTTTATTCTCCAATTGTTTTATTAGTTTGTGGTGCAGGATTGGTTTGCAGTGGATTTAGTTTGCAATCTACAACGCGTAATGGTTTGGCAGGTCCATCAACATTAGGAATTTTTCCATTAGCAGCCTTAGGTAATATTTTGAGTCAGTTAATTACCAAAAGTTTTTCTAATATTTTTATTGGCTATGTTTTTGGAATTATTTTTAGCTTATTTGCTTTAGGAATCAATTTTTTATTTATTAGAATTTCACCACATAAGAAAACTTTTAAACCTATTATTTTTGGTTTTTCATTAGGTGCCATTATCACAGCTGTTAATATTTTAATTGCTAATTTTAATTCATCAATTGTCGCTTCCCCCATTCAATTATTAGGTAGTATTGGTGTTTACAATTCTATTGAGCGCTTTTATATTGGCACTCCAATAGTGTTTATAAGTACGATTATTTTGTTATGTTATGCTAAAAAAATCCAAATTTTAGATACGGATCATTATTTAGCTAAATCACTAGGAATCGATGTTAATAAAGTGTTTTGAATCACTTCAACTTGTTCAATTTTTATTGCCATTTCAACTACGTTTTTAATTGGCGGTCTTTCATTAATTGCAATTGTTATGCCCCATTTAGTTCGTTTACTTTTTAAAAGAACTAATTATTTATTTCAAATGATTATCGCCATTTTTCTAACTACTTTTTTATTAGAATTACTTGGAATAATTGTAGAATTTTTTTCAAAATTTAATATTGTTTTTTTAGTAGCAACAGTTTTTGCGATTCCAATGATTGTTTTGCTAAAATGAGAATTAGGATGGAAAGTGAATAAAAATTAAATGAGTATAAAAATTAAGCATGATCAAAATTTAAAACACCAATTTGAACAAAAATTATTTTTTCGTTTTAAAAAGAACAAATTTTTTCTTTCATTAAGTTTATTAATAATAACAATTATTTTAACGCTGATTGCTGTAGCTGGAGCCTCGCGTTTTATAAATGTTTTTAGTTATTTGAGCGATTGAAAAATTATCACACAATTATTGTTTTCTGGAATTGCATTAGGAATTAGTGGTTATATTCTTCAAAGATTAACAAAAAATCGTTTAGCAGATAGTTCTTTATTAGGAATGGGCAATATTAACTTAGTGATTTTAACAATACTTTTTTTAATATTTGATTTTGGGCAATTGCAAATCCAAAAACGAATGGAATATGTTCTACCATTTATTTATTTAATTGGGTCAACAATGATTTGTTTTTTTATTCATTTTTTATGCAATTCTTCTACTGGATACATTTTTAAAAGAATTATTGTTTCAGGTATTATTATTAATTTGATTACAATTGTTATTGCTCAGTCATTAAGAATTTTAATGAGTAAAGAATCAAGTATGTATTTAAAAACAATTTTATTAGGTAATATTGAATCACGCAGCGATTTTTGTTTTTATTTTTGTTTTGGAATGCTTACTATTAGTGTTATTTGGTTAATGTTAAATGCAACTAAATTTAAGATTATGGTAACAAATCAACAAATATCTGGACAGTTAGGCATTAATAATAAGTCCTTGACAATTCAAACTTTTATTTGCATTAGCTTATTAGTATCGGTAAGTTATAGTTTAAGTGGTAATGTTATTTTTGTTGGGATTGTTGCAGCTAATTTTTCTTTCAATTATGTAAAAAACAATATTTCTAATGGCATTATTAATGCTGGATTAATAGGCGGCATTGTATTATTGATTAGTTATATTTTTGTTGTTTTAATTTTAAATTTATCAACTGACCAGACTATTTTATTAGTGCCAATTATTTCAGGACCATATTTTTTATATCAAGTTATTTTATTGAAAAATTAAATTTATACTTTATCTCATTAAAGATTGGTTTTTTATAATAAAATATTAACTATTAAATAAGTTGATTATTTTTTTATTTAAAATTAAAAAAAGAAAGTAATGATTATGGAATTAATTAAGACTAACACACTTAATTTTTATTTTGAGCCATCTAAAAGCGAACTAAAAAAAGGTTCGATTGTTTTTATACACGGCCTTGATGCATCTCCACATTATTTTTTTTTAATTAATAAAGATTTATCTGATTATGATTGTTATTTTGTTGGATTACCTGCACATGGTTTAACACCTATTAATAACAAAAAAGATTTAAATATTAAAGTTTTTGCTGAGTTATTTATTAATTGAATTAATGAAATTGATTTAAAAGAATTTCATTTGTTAGGTCATTCATTAGGAGCGGGGATTGCTAGTTTGGTTAGTTTTATTATCCCCCAACGCATTGAAAAATTAATTCTTGTTTGTCCATATCATTATCAATATTTAAATCCGTTTTTAAATAAAAAATTATTTAATGCTTGGGTTTTATTTCCCAATCCATTTTTAAAATTTAAAACAGATGTCGTTTTAAAAAAATTATATATTGATTATCGTAATAATTATAAAACCTTAATAGAAACTCGTTGGGAGTCAATTTCAAGAGAATATCCACGAATTGCACGCGATGTCTTATTTTTATGTTTATCGTTATTAAATATTAAAATCAATCATGAATTAAAAATGGCACAGCGTAATTTAATAATGCCAACATTAATTATGGTTTCAAGACATGATCAATTAATTGATTTTAATTTAGCTATTAAAGTGTTTAAAAACAACAATAAAGTAAATCAATATATTTTTAATAATTCTGGTCATATTCCTTTTATTGAAGAACCAAAATTATTTACTAATATATTACTAAGTTTTTTAGAGGATAGATTTGTAGAACAAGAGGAAAATGATAACAATGATATTAACGAAAAATAGTAATGAAAAAAAACCGCTTAAAAAAGGATTATTTATTGTTTTTGAAGGAATTGATGGTGCAGGGAAAACTTCAATCTTAAAACAACTTTTAGAGGTTTTAAAAGAACCTAAACTAGTTAATAAAATTTTTTTAACGCGTGAACCTGGTGGAAAAAACAATAATGCAGCAGAGATGATACGTGAGTTTTTTTTAAAAAATTTAGAAGTTTTTGATCCGTTAACACTAGCTTATTTATATGCAAGCAGTCGTGCTGAACACGTAAAAAAAACAATCAATCCTCATTTAGAAAAAGATCATATTGTAATTTCAGATCGTTTTGTTCATTCTTCATATATTTATCAAGGTATTGTTCAAAATCAAAGTTTAGATGTTATTTACCAAATTAATCAACAAGCAATTGGTGAATTAGAAATAGATTATGTTTTTTATTTTGATGTTAATGTTAATAATGCATTAAATCGAATGAAAAATCGTTTTGACAATACAAATGCATTCGATTCACAAAATAAACAATTTTATGAAAAGTTATTAAAACAATACCCTAGTGTTTTTAAAGTTTATAATCAACCTAAAAAAATTATTTTTATTGATGCCAATAAAAATGAAAATGAAGTATTATGTGAGGTTAAAGAACAATTATTAAAAATTTTTAAAGAACATAAATATATATAAATGAAATACAGTTTTGCTAATTTATTAATTCAATCACCTAAAACCTCACTAGAATCAGGTATTGAAGAAATTATGTTAGCTTTTATTTATGAGAAAAATCATAAAGAACAATCATTTTATGTTACTAAAGTTAAAAATAAACAATACTTTGATTTAAAAATTTATGATGGACTATCAATGAAAAAAAGCGATGTAATTGATTTACAAAACACTTTTTTATGTGATGGAGTAGAAGATATTAATTTAAAATTTTATCTTATTAAAAATATTGACTTAGCATCAAAATATGTTTTAAATGCATTATTAAAATTTATTGAAGAACCGCCAAAAAATACAATTGCAATTTTTAGCACTAAAAATTTAAATCAAGTTTTAAAAACTATTAAAAGTCGTTGTCAGCTTTTTTATTTACCTACTAATCATGATTTATATAAAAAATTAATTAACCAAATTAATCAACCAATTGATCTCGTGGAATGCGACTTAATGTTTGATGATTTGGATGAGCTAAAAATTTTATTAGAAAACAAGGAAATCAATGATGTTTTAACTTATTATGGAAAATTAAGTGATTTAAGAAGTTTTGAAATATTAAATGATTTAAAAGAAATATTTAAAAATTTATCTACCTTACAAATTCATTATCTATTAAAATTAATCTTCATTAGAATTAATAATGTAAATAGCAAAGAAATTATTTTGGATTTAATGCGTGCGAATTTAAAAATTAATATTAATAAAAATAATTTGTTTACTATTATTTATACAATTATTAATAAAAATAAAGGTGATTAACAATGTCAACAATTGTTGCTTTAGCAACCGCGCCTATGAATTGTGCCATTCACATTATTCGGGTTAGTGGTCCAAAAGCTTTTGAAATAATTAATAAAATTTCAACAATAAAAATAAAAAAAGAAACTTTTAAAATTTGGTATACAATTCTAAAAGATGATAATCAAATTTTAGATGAAGTTTTAATAAATACATTTGTAGCTCCAAAAACATTTACTGGAGAAGATTTAGTAGAAATTAATTGCCACGGAGGAATAGTGGTTGCTAATTTAATTATTAAAACATTAATTAAATATGGTTGTCAACCAGCACAACGTGGAGAATTTTCTCGCCGTGCTTTATTAAATAAAAAAATGGATTTATCAAAAATTGAAGCAATTAACAATTTGGTTAATGCTAAAAATGAATTAAGCGTTAAAGGTGTAATTGGCGCTCTATTAGGACGTGTTTCGCAATCAATTGCTGAATTTAGGCATGAGTTATTTATGATAATTGGGCAAATTGAAGTTAATATTGATTATCCTGAATACGATGATGTTGAACAAGTTGATGCTATTATTTTAAAACAACGTTTATTATCTTTAGATAAAAAAATTACAAAAATTATTGATCAATCAAAAAAATTTTTACCAATCAATAAAGGCATTAGGGTGTTAATAATTGGAAAACCAAATGTAGGTAAATCAACTCTATTAAACGCTTTATGCAATGAACAAAAAGCGATTGTTACAGATATTCCTGGGACAACACGTGATGTTATTGAATCCTCAATTAATATTGATAATATTACTTTAAATATCTTAGATACTGCAGGAATTCATTTAACAAATGATTTTGTTGAAAATTTAGGAATTAATAAAGCCAAAGCATTAATTGATAAAGTTGATTTAATTTTATATTTAATACCTGCAAACGAACAACAAGATTTGGAGTTATATGATTTAATTAAAAAACAAAAACATTTATTAGTTTATACAAAAAAAGATTTAGTTGATCAATACGATGATAAACAAATATATATTAATGCAAAAAATAATGATATTCAGTCATTGATTGATGAAATTAAAAAACTTTTTTATGTTCAAGAGTTTGACAATGCTAACATTGATGTTTTACAATCCCAACGTCAAATTGGTATTTTAGAAAATGTTCATTATTTAATTAATAACGCAATTCTTAATTTAGAAAAAGGGGATACACTTGATTTAATTGTCGCTGATTTAGAATTTTGCAATTTACGTTTAAATGAACTCTTAGGCATAAGTAGCGAATATGATTTTTTAGATGATTTATTTAAAAATTTTTGTATAGGTAAATAATATGTTAGAAAAAATAAAAATTATTGATACTCATACTCATCCTAATATTGAACCATTAATTGAAGAATTTGATGACATTATCTTTAAATGTTATGAACAAGGTATTGGATTAAATATTGTTGGAGTAGATTTAAAAACTTCACAGACAGCCATTCAACAAGCTAAAAAATTTAAATTTTTGACTTGTAGTGTTGCAATCCATCCTAATGATTTACATAATATAGAAAATGATTTTGTTGCATTAGAAAAATTGATAGCAGAAAATATTGATTACATAAGTTGTATTGGTGAGTGTGGTTTAGACTATTATCATGAACAAAATTATGATCATGATTTTCAAAAACATTGGTTTAAAAAACATATTGAATTGGCTAAAAAGTATCATAAACCATTAATGTTACACATCCGTAATGCACACGATGACGCAATTGCCATCTTAAAAGAATTAGCTGTTCAAGATGTAATAATCCATTGTTTTACAGATAAAAAAGAGTATGCACAACAATATATTGAAATGGGTTATTATATTTCATATCCTGGTGTAATTACTTTTAAACCAACAAAAAATAATCAATTACATGAACTGTATGAGGCGATTAAAATAACCCCACTTGATAAGATTCTAGTAGAAACAGACGCCCCATATTTAACACCAGTTCCTAAACGAGGGCAAACTAACTATCCTTATTATGTATTATATACATCAGCATTTATTGCCAACTTATTAGGCATTACACATGAAAAAATGCAAAAAATCTTATTAGAAAATTCATTAAGAATTCTAAATTTTAAAAAATAAAAAAGTGTTTTAATTAAAAAAACAAAAATTAAGTGTTTAACTTATATATACAAGTTTATTTTTTGTATAATGATACTACACAATCTTAAAAATTGTGTTGTTTAGTTATTTTAGATTTTTCATGTCAAAAAGGATAAAAAATAAATTTTATGAAAAAAAGCAAAATCAATAAAAAAATCTTGTTTGCATCACTTGCTGGAGTGGTTACATTAACTTCAGTTGCAGCTATTGCTGCATCATGTAATGATAGTAATAAAGATGATGGGAAAACAAATAAAGATGGAAATTATATTTCTAAATTGTCTTTAGAAGATTTTTATGCAAAACCAGCTGGAGATGATAGTTTAGGATATCACAGAACATATAATTCATTATACGATGATGGTGCTCGTATGTTAGGCTTGATTTCATTTAGTCACTCAATTCCAATTAAAGAATATTTTGGTTCAAGCAGCGATAAAAAAGATTTAAGTGCAGTTTTAATTGATGATAAATTTAGTGGAACAGTGGGTAAAGACCGTATTGCGTCTGTCTCATATCGTGTAGATCAAGCAGCATTTTTAACAGGTATTGCTGCGGCGTACTATTTAAATGCTAACCAAAAAACTTTTGCAGCAGATGGAAAACTAACTTGAGGTGGATATGTTGGATTACATTTTACATCAACTTCAACTTTTATCCAAGGTTTTAAATTAGGTGTTCAATGAGCAAATGAAAAGTTAAAAGATAAAGAAATTAATCAAGAAGATGCTAATGGAAGCAAGAAAAAATGAATGAATGTTGAACAAGTATTTGCTAGCAAATACGTTGCTGGTTCATTTAAACCAGATGAAGAAGGTGCAACAAACATTATCAATGATTTAATTACAAAAAAAGCTGATGTTATTTTACCTGTAGCTGGTCCACAAACAAACCTTGCAACTTCAATTGTTTCAAATGCAACAGATCCAAGTGTAATTATTGGTGTTGATACTGCACAAGAATTAGATGATGTAACAAACCGTAAAAGAATTACAAACAAAACTGTTAATGATGGAAAAACAATTTTATTCTCAATTGTTAAACGTGTTGATTTAGCAATGAAGGGAGCAATTGAAAATGCTTCAAAAGGTGCACAACTAACTAATGATATTAATAAAGATGCTTATAAATTAGGAACACATACTGAAGCAAGTTTAGATAAGAGCACATATGTAGATGATACTCCGCTTGTTGAATTATCAAATGCAGGTCGTGTTTATTTAGAACAAGCGGCTAAACTAGCTGGTTTAAAAGCAATTACATATGCTCAAATTGTTAATGTTATTCAAAATGAAGAATTATTTAAACTTCTTTCAACAAAAGGAACTACAAAATTAGAAGATCTTGCTACAAAAACAAGCGATGGATGAGTTTTAAAAGATAGTGAAAAAAATAAATCATTTAGTGAATTACAAAAATTACTTGGTGGTGAAGTTTATATAAATGAATCAGATAAAAAATTATATCCATATTCATTAACAGGTTCTTCATATTTAGAAGAGGATCCAAAGAAACGAAGTGCATCACAAGAATTTAAAAAATATTGAGATGCTGCCACAACACCAGAAGCTAAAGAAAAATTAGCAAAAGTTGTTTTAGGTCAAAATAATGCTGTTCTAAAAGATAAATCTTTTTCAGAATCTGCATATAATGGATTAGCAGCATTCTACAAATCTAAAAAAATCATCATTCCAAAAATTTAATACTTTAAAAGTATCTAATTTGAAATAAATATAAAAAATTATAGAAAGTGATCATATGGAAATTAAGACAAATCAATGTCCTTACGCCATAGAAATGCACGAGATTACAAAAACTTTCCTGAACGGGACTGTTATTGCTAATAAAGATGTTAATCTTTTTGTTAAAAAAAATGAAATTCATGCAATTATTGGTGAAAATGGAGCAGGTAAATCAACCTTAATGTCAATTTTATTTGGGATCTATAAACAAGATTCTGGATCAATTAAAATTAATGGTCGAATTGTCAATTTTAGTTCAGCTAAAGATGCTTCAAAAACCGGAATTGGAATGGTACATCAACATTTTAAATTAATTGACACCTTAAGTGTTTTAGATAATGTTATTTTAGGTTCTGAAGGTGCAACGCAACTAGGTATCATTAACCGTAAAAAAATTACTAAAGAACTTAAAAAAATCATTCAAGAATATGGATTAAATATCAATTTAAAAAGTAAAATTTCAAAAATTACAGTTGGTCAACAACAAAAAACGGAAATTTTAAAATTATTATATCGTGATATTGATATTTTAATTTTTGATGAACCAACTGCTGTTTTGAGTGAAGATGAAATTCAAGCATTTTTACAAATGCTAAAAGATTTTAAAGCAGCAGGCAAGACAATCATTATTATTACGCATAAACTAAATGAAATTAAAGAAGTTGCTGATTCTGCAACAGTTATTCGTCGCGGACATTATATTGATAGTTTTGATGTTAAACAAAAAACAATAGCTGAAATGGCTGAATTAATGGTTGGTCGAAAATTGGTTGAAGTTAAAAATATCGATCCAATCACAAGTAATGAAAAAGTTTTCGAAGTTCAAAATTTAGATATTCAATCAATTGTTAAAAAACAAAAAGATATTGTGAAAGTTAAATCATCACCAAAAGGTGATGATGTTTCATTGTTAAGTACTAAAAATAATAATTTTATTAATTTTAGTATCCGCAAGGGTGAAATTTTTGCTATTGCCGGTGTTGAAGGTAATGGTCAAAGTGAATTAGCGCAAATAATCTCTGGCTTATTAAAAGGAAATAAGGAAGCAAAAATTATGCTTGATAATCAAGAAATTGAACATGCAAGCATTAGAAAACGTTATCAGTTAGGATTATCACATGTACCAGAAGATCGTCATAAACATGGATTAGTTTTAGATGACACGATTGCTATGAATGCGATCTTACAACAAGTTGATGATAAACCATATAGTAATTTAGGATTTTTTAATAATAATGAAATTTCAAAACATGCAATTAATATTATTAAAAAATATGATGTTCGTGGTACAACACGTGGTACATCAGATGCACGAGGATTATCTGGTGGAAACCAACAAAAACTAATTATTGGTCGTGAATTTGAACGATCTCATAAATTAATTTTATTGGTTCAACCAACAAGAGGTTTAGATTTAGGTGCGATTGAATTTATTCATGAACAAACATTAGAAGAGAAACGAAAAGGTAATGCTATTTTATTAATTTCTTACGAATTAGACGAAATTTTATCACTTGCTGATACAATTGCTGTAATTCATAATGGATATTTCATTAGTGTTGGTGATCGTTTTTCAATGACAAGACAAAAAATTGGTGAATTAATGGCAGGTGAAAAAATATAAGTATGTTGAAATTTAAAACTCTTTTAAGTTACGATAGCTTTCTTAAAGGTCAAGAGCGAAAAACAATCACTAAAAAATTCTTATCGACAGTTTTTGCCATTTTTGTAGCGATAGTAGTTTCAGCTATTTTGGTTGGAATATTAGGCTATAACATACGTGATTTCTTTATAAGATTATTTACTAAATGAATTGGTTCGCCAGATATTTATTTAACAAAAGTAGCAATTTTAGGAATTGCCGCATTATCATTTATTTTCGCTTTTAAAGCGGGATTATTTAATATTGGTATTTCTGGACAAATGTTAGGTTCAGGTTTGATTATGTTATTAGTCGTAAAAAACATGCAAATTGCCAATATTAATATGCCTAATGTTTTAGGTCAATTTTTCTTATTAATCATTGCAATGTTAGCAGGAGCAATGTTTGCAGTTTTTATAGGAATTTTAAAAGTATTTTTAAAGATTAATGAAGTTGTTAGTTCAATTTTATTGAATTGAATAATGTTCTATATTACGCGTTATGTTGTTTTTACTGCACAAAGTAAAATGTATAATCCAACACCAGATCAAGTTGGATCACGATCAATTCCTTTTGCTGATAATTATTCTTTATTTCATTTAGCATCTGGATATGGTTATTTATTTGCTTTATTGATCTTTATTTCTTTATCTATTATTATTTGAGTAATCTTAAAATACACAGTTTTTGGTCACAAAGTCTTATCTGTTGGTAAATCATTTGATGCAGCAAAATATGCTGGATACCGAACAAAAATTATTTCATTAGCAACTTTTGCGATCTCTGGTGCATTAGCTGGGGCTTTAGCAATGGTTAATTATACTTCAACAACAACAAATGCGATTGTTATTAGTCAAGCTGCTGATGCCTTACCAACCCAAGGATATGATGGAATTGCGATGGGATTAATTTCTCTAGCACATCCTTTAGCAACAATTCCTGTCTCATTCTTAATGGGATTATTACAACAATCTGCTGATAATTTAGGAGGAAGTTTTCCACAAGATTTATCTGGAATTATTATTTCATTTGTAATGTTAGGGGCAGCAATGTTTATTTTATTTGAACGAATAAGTCCCGTTTATTGAATTTATCATTTAATCTATACTTATAAAGGTAAGGATTATTATCGAGATTATGAAAATAAAAACAATAACATTATTTCTGAATATAAAGCAATCTATAGTGAAATTAATAAAAAACGTAATCAATATTTACAAGAGTTAAAGAAATTACGTAAAGAATTAGATCAATTTAAAAATCATGAACAATATAACAAAGTTAACCAACAATATGAATATACTATTAGTTACATTAAAACAACATATAATGCTTGATTAAAAGTTGAATATGATAAATATTTGAAACAATTGAAAAATAATGCAATGCAACTTAAAAAACATTTAATTATTGAAAGAGCAACACTTGTTTATTATCCAGAAATTAACACAACAAGAAAAATTAAAAATAAACTAAAACATTTTGAAAACATAAGCGCTAAAAAACTAAGTCGTATTAAAGACAAAATTCATAATGAAGATTTTTTAATTAAAAATTTAGTGGCTAAAGAAATTAGTAAATATGCTCAATCAAAATCGTTTGATTATAATAAGCTAAAATATTTATTACAAGAATATGAAAATAACAATTCAGTGTTAACAACCGAGTTAGTTAATGAGTTATCTTTAGTTCTAAAAGATGAACAAGCACAATTAATGTTTAGTGAATTTAAACAGGCAAGTTTTGAGAATAATTTAGATCACATGATTGATTTAAAAAATCAAATTATGACATACATTAATTCTTTATTAGATTCTCGATTAGAACAATTGTCAATAACTTTAAAATCAAAGAATTTAGTTCATGATGAACAAATGGATAAGATTATTTTATTACTAAATCCAAATGATAAAATTCTTAATAAAATTATTATTTCGCGTAATAAACATTTAGAAAATTACACGAAAGAAATTACAAAATTAGAATTAAGAAATATTTCGCAAGATGATGAAATTAAAAAGAATACAAAACAACGCTCATCATCATACTTGACAAAACATTATGAAAAAACTAAACAACATATTAATAAAATTAATCTAGATCAAGAACAAAAATTATTATTAGAAGATTGATTAACAACTGCTTATCAAAAAGCAATTATTAATCAACAAAACATGAAAGAGGTGATTAGTTAATGCCTTCATTATCATTAATTAACGATGCCTCTATTTACGCTGCTATTTTAATTCTAGGTGCGTTAAGTGGTTTCTTTTGTGAACGTGTTGGGATTGCTAATATTTCAATTAATGGTCAAATGATTATTGGTGCCTTAGTCTTTACATTATTTTCAACAATTATTTATAAATATATGGGAACAAAAACGGAAACAGAATATACATTTATTGTATGTTTAATTATTGCTGGAATATTGACTATTCCAACTTCATTATTATTTGGTTTTTTAACTATAAAATTAAAAACCAATCAAATTATTGCTGGAACAGCTATTAATTTATTAGCTTCAGGAGTAGCAACCTTTGTAACTTATCCTTTGGGCGATAAAATTGCTGACAAAACAAGTCTATCTTCTGATTATTTAGGATTAATGAAAATTGGTGATGGAATCAACACTATTTATATTGGAAGTATTTTAATTTTAATTGCTGTTGTTTTAATTACCATTGGTTTAATGATAATGATGAAAAAAACTCCATTTGGTTTAAGATTATATGCAATTGGTGAAAACCCAAATGCAGCAGATGCACAAGGCATTAATGTTTATAAATACCAATGAATTGCTGTTTCGATTTCTGGTTTTATTGCTGGAATTGGTGGTGGATTATATATGTATGCATCACGTTCTCCATTTGCAGGCGAGGTTGGTGGGATCGGGTTCTTAGCTTTAGCCATTTTAATTGCTGGAGCTTGAAGAATTCCACTAATTGCAGTTGTTTCAATTGCTTTTGCAGTTATTACAAAAACCTTTTCACAAATTGATAAAATACCTCAAGAAATTGGTAAATTAATCCCCTATGTAATCACTTTAATTGCTTTAATTTCATTTAGTAAATATAGTGTGGCACCAAAGAATGTGGGAATCCCATTTGATAAAACAAAAAGATAAATTAAAATGTGCTTATTTGCACATTTTTTTATTATAGACTAACCATTAATTTCATATTTTTTTAAAGAAATGATAAATTATTTTTTATGATGAAGTAAGGGTTTTAATTTGATTACTTTTTTAATTATTTATTTTTTATCATTCAATTAAATCTAATTAAAATAAAGGCTTTTAGTAAAATTATCAATTTCATTAAACAAATTTTATAAAAATTAGTTTTATGACAAGATAAGAAAAAAAGGATTTTTTCCTTTATTTTTTTTAAAAAAATGATTTTAAAATTTAACTTTTTTAGGTTAATATTATTAACAATAAATAAACGATTTTAAATTGTTTGTTTAGTTATTCAAGATAAATTGTGAATAATAAAATATAAAGGAGTAAAATGAAAAAGTTAAATAAAAAAGTTCTATTTTTAGCAATTGGTAGCGTTTTTGCTTTAGGTACAATTATTACTGTAGCAACATCATGTACACAAAAATCTACATTAAACTATAGTCAATTTTATTGGACTTCACCAACAAGTGATGATGATGAAGGTTTTCAAACAAAATATAAAAGTATGGCTAGTGATGGTAAACGAGCTTTATTAATGCCTGGTTATCAACATCCTGAAAGATTACAAAATGCTTTAGTAAATGATAAATTTGATCCTAATTTAATCGCTTTAATTTTAGATGGAGTTTATAATAATGACAATAAAGCTGAGTTTTATAAGGGAGCAGATCGTGTTGCTGATGTTTATTTTAAAGTTGATGAAGCCGCTTTTTTAGGAGGTATTGCTGCAGCTTATATGTTAAACTCAAATCAAGCAGTTTTTGGTGCAGATAATAAATTAACTTGAGGTGGGTATGTAGGTATAAATGCTAAAAATACAACCAATTACTTAGCAGGTTTTGATTTAGGTGTTAAATGAGCTAATGAAAAATTAAAAGATAAAAACATTAAACAAGAAGGAACACAAGAAACAAAAAAATGGATTAATGTTGAACAAGTTTATGCTTCAGAAAGTAGTGCTGGAGGTTTCCAATCTGATAGTGCTAATGCTAAAAAAATTATTCAGGAATTAATTACTAAAGGTGCTGATTTAATTTTACCAGTAGCTATTCCTCAAGTTGGAATAGCTGTTACTGAAGCAATTGCAACAACTTCACACAATGTAGGAGTTATTGGTGTTGACGTAGAAATTGAAAACGATCAAGCAATCAATAAAAAAACAGATAAATTTATTAACACACATCTAAGCGGAAATAAAAACGGTGTTATTCGTTTTTCAATTACAAAACGTTTAGACACAGCAACGATAAAATTATTAGAAAATGCAATTAAGGGAGAATCATTATCTAAAAAATCTGACCAGATTGTTATTGGTAGCGAAATTGATCCAAAAGATAAATATAAATTAGGAGTTAATACTGTTGGTAATTTATCAGATGGTGTAGTTGGTATTTCACCATCAGCTTATCATTATGTAATTGATGCATTTAATTTAGCACAAACAAATCAAAGTGATAAAGTAAGCACATATGATGAATTAGTTAATAAAATCACTAATGATGATTTATTTAAAACACTTGATAAAAAACCAGTTGTTGATGGTTATTTAGATGTTAAAAAAGAAACAGATAATGAAGCATCTTTATTAAATAATAAAACGATTGATCCAAGAGTAAATGGCGGAACTTTTTATAAATCAGCTAAAGGTACATATTATTTCTATCCTGTTGCCAAATCTACATATATTTCTAACACATCTTCAAAGAAATTTAAAGAAATATGGGATAATACTAAATCAAATGATGAAAAACAAGCATTAATTGGGTTGCTTTTATCTTATGCAGGAGCAAAAGTAAAAGATGGTGGATATTCTGAAAATACGTACAACGGTCTAAAAGAATTCTATGCAAAACATGGAATTAAGATTCCACAATTATAATTAAAAATAAAACATTAAGTATGTGTCTATTCAAGATAAAAAATGAATTAGTATAACGCTTAATGTTTTTTTATCATCATTTATAACTATAATAACTTTATAATTTAACTTAATAAAATATCTAATTAAATTTGATTAGAACAAAAACAAATAGAATTAAAAACCGGTATCCTTTATAAAAGATGCCGGTTTTTAATTAATAACAATTTATAAAAAAGAGAAACTTAAACAAGTTGTACTTTTGCACCAAGTTCATCAAATTTCTTTTTGATTTCGTTAGCTTCTTCTGTTTTAACATCTTCTTTAATTACACTGCCTGCAGTTTCAGCAGCAGTTTTAGCTTCCATTAATCCTAATCCTGTAATCTCACGAATTAATTTAATTACACCAACTTTATTAGTACCAGCTTCAACTAATTTAATAGTTACTTCAGTTGGAGCTTCAGCGGCAACAGGAGCAGCAGCAGCGGCAACAGGAGCAGCAGCAGACACACCAAATTCAGTTTCGATAGCTTTAACCAAATCGTTTAATTCTAGCATTGACATTTCTTTAATCGCAGCAATAAATTGTTCAATTGTTAATTTTGACATATTAAATTCTCCAATAATATTATTTTATTCACCTTTTTGCTCAGCTACAGCTTTTAAACCATACATCACATTACGTAATGGTGCTGTGAAGCAAGAAAGTAGCATTGAGTATAGACCTTCACGGCCAGGAATTGCAGCAAGTTGATCGATTTTGTGAGTATCTGCAAATTTGTTTTCAAGATATGCACCTTTAATTTTGACAAAATCAAATTCTTTAGCTACATCATTTACTGCTTTAATCGCAGCAATTTCATCGTCCATTGCAACAGCAACTGCGTTAGGTCCTGTTAATTGATTTTCAAATCCACTAACACCAGCTTTTTCAAAAGCACGTGCTGTAATATTATTTTTTAAAACATATAATTTAGAATTTGATGAATGTAAAACATTTCTTAACGCTAAAATGTTAGCAGCAGTTAATCCATGATATTCAAAAACAATAAAAGATTTTGAATTTTTTAGTATATCTGCCATATGGTTGACTTCTTGTTGTTTAAAAACAACACTTGGTCTAACATTTGCCATATTTATACCTCTTATTTAGAATATGTGTTTGCAACATGCATTTTTGTTTAATATAAACCTCGGTAACAAATTAAGTTTAATTAATAAACAGTTACTTTCTTAGGCATATTGCTTTTAAATTATAACATTTTATAAATAATTATTTCTATAAAAAAATTAAGCAAAATGTTTTGAATAATTCAAATTATTTAATAAAAAATTTTGATTAGGTAATCATGAATTAACATTTTTAATAGCATGAATATGCTCGCGGATATTATTTAGTTGTTCGACTTTAACTTTATCAACAATAATTAATAAAAATATAATACCAAAACCAAATAGGATAATAGTTGTAACCATATCAAGTAATGTCGATAAAATTGGTGTTGACATAATTGTAGGATCAATTTTTAATTTTGTTGCTAAAAGTGGCAGTGTTCCACCAAGCAATTTAGAAATTACAATAGCAAAAAACAAAGCGACTGATGATCCAGCTGAAACAATTGCTCCTACAATCATTTTAGTATGTGGATTATTAGTAATATTTTCGTATAAAACATTGTATTGTTGTAAATCAGGTTTAATAATAGCAAAATATATAATCAAACGAGCAAAATTAATAATTGCTAAAACTAAACCAATTAAAGCACCAACCAAAAATTCTTTGGCAAGGACTTTATTATATTCTTTTAAAGTAATTTCACCAACTGATAAAGCACGAATCACAGAGGCGGCAGCTTGTGATCCAGCATTTCCACTTGTTCCCGTCATTGCTGGAATGATTGGTACGATAACTAATGTTGATAAACCTGCTGTGACTAATTGTCCTACATCTTGAAATTTATCAATTAAAAAACCAGTACATGTAGCTGAAATCATTAATACAGCCAATCATAATAGACGTGATTTAAATAAAACAAAAACACTTGATTTAATGTAAGGAAAATCTAATTCGCTAATCCCATACATTTTATAAATATCAGTAGTTGTTTCAGCATTAATTACGGGTAAAATATCATTATCACTAATATAACCAATTAATTGGTTATCTTTATCAACAACTGCTAATTGTTCAATTTGATACTTTCTGAAAATATCTAAAACAGCATCAATATCTTCATCACTACGAACATAGAAAACATCTTCTTGAATAACATTAGAAATTTTCAATTCAAAAGAATCTGCAAAAAGTAATGAATGAATTGTTACATATCCTTTTAAACGATTATAATTATCTACTACATATATAATATTACTTTGTTCAAAGTCATTATACATTCTTTTTATTTCAATTAGTGCTTCTTTGATAGAAAGATTTTGATTGATTGTAAAAAAGTCAGAATTGATGATTGAACCAGCTTCATCTTCATCAAATTCAGATAATTGCTTAATAGCTGCTCTTTGTTGGCTGTCTAATGAAAGTAAGATTTTTTTAGTATACATAGGAAAATCATTTTTTAAAATAATCACATCATCATTATAAAGTTCAAATAAAATGATTCTTAATTGCTGTAAACTTGCGTCATTTATGATTTTTGATTTTAATTCAGTATTTAAATATTTAAAAATATCACCACAAATTCCATCTTTTGTGGCAACTAATACAAATAAAATAATTTGTGTATCATTAATAGTTTCAAGACTTTCAACAATTTTATCAATCCGTTTTGATTTTAGTAATTTTTTAAGTCCTGTAATATCTTTATGTTCGTAAGTTTTTAAAATATTTTGTGTTAGAAAATTTTGATTAATATTTGTGTTGTTATTTAAATTTAATGTACTTTGTTTATTTTCCACAATAAACCACCTCCACAAATAAAATTTATAATTTATATTATAATAGACATTAACTTAGTTTTATAGATAATAAACAATAAAAAAAGAAAAAAGAGTTTTAACTCTTCTCTCTTTCTTCTTCTTTTTTTAAAAAAAGTATTAAATTTATCTTTTAGGTAATTTTTTAACAATTGGATAGTGTAAGTTGCAGTATTCTAATTCAGTCATACCAGCGGCTTTAGCTTTAGCTTTATTTGCAATTTTTAAAGCAAACACAATTATACTTGCAATGCAAGATAGTGTTTCACTTAGAGCTACAAGAATAAATCCTCCAGGGTTTACAGAAACACCTAATCAAGCAAATATCGCTAATAATCCTAAACCAGCAATTGAAATAATTCACATACCAATAGCCAAATTTTCAGTTCTTTTTGAAATTAGTACTTTAATAACAGCAGGTAAATAAGCACCAAAAACTAAAATAGCTCCAACAACTTGAAAGAAAATACCTCAAGGGTGTGTTGGGAAACCATATGCACCCTGATAATCAGGTGTAATTCCTAAATATGGTGATTGGTCAGTAACGATTGCACCATTACCAACTTCATATATACCATCTTGCAATAATGAAGTGAAATTCATAATTAGTTAGCTCCTTTCACTTTTAGGAATGGTTTAATTTGTTTTTCATAATATTCTTCTTCGCTCATTCCCATTTTTTTAGCTTTAATCATGTTATTAACTTTGCAGAATAGAACAATAAATGAAGTTACTGAACATAATGCTTCACCCATAATTAAGAAGATACCGCAGATTAAGTATCCAGCAACTCACTCTTCTCTATTATAAATGAACATTCCGTTTTTAATATCCACTAAGAATGAAAATGAATTTGGATGAACTGTAACAAGTCCAATACCGTATAGAGTTAAAAAGGCTGTAGCAATACCATTAAGCAAGAATAGTAATAAACTTAATGAAGCTGTATCTTTTTCACGTAAAGTTTTAATTGTTTGTGGCAATACACTCAAAGCAATAACGATCGTACCTATAACACTAAAAACAGAAATAGCGATACTAATTCCTGAAGCGGTTAATGTTTCTGGAACTAGATCATTGATCAAATTTGAACCAGTTTGCGCAGTAGCATTTGAAGCGGCTGAATATAGTGAAGAATTAGATACATTATTTAAAATATTTAACATAATATAATCTTTCTTTAATATTAATATTTTATTGCACTTTTATATTACTACAAAATGAACACAAAATGTTAAAAAAATTAAAAAAACTTTTAAAATTAACTATTTTGCTCTTTTTCATATTTTGCAATTATATCTAAACGTTTTTGGTGACGCTCGCCTAAAAATTTACTTGTTAAAAAATCATCAATCATTTTAAGAATTTGGTTTAAACTATTTTCACGTGCTCCTATAGTAATTATGTTAGCATTATTATGCTCCTTGGCTAGTTTTGCACTGCTTTCGTTATACACTAACGCAACCCGCGTTCCATGCACTTTGCTTGCTGCTATATTCATACCTATTCCCGTGCCACATAATAAAATACCTATGCTATCATTTTTAGTATCATTAACAACGTCATTAGCAACTTTTAATGCATATGTACTATAATCAACAGAACCTAATGCGGCTTTTGCACCTTCATCAATAATTTGATAACCTTTTTGTTTTAAATGAGTGATAATTTGATCTTTAATTTCATAAGCAGCATGATCATTACCAAAATAAATTTTTTTTATCATTGATTATTTCCCTGTTTTATTATCTTTTAGTTCTTTAATAAACATATCTACCATCTCAACATTAGCACCACGACGAATGTATTCTCATTTATCGATGTCAACAATAGTTGATGGAACACCAATTTGTTGTTGTGCTTCAACATAAATTAGTTTACTATTTGCTCCAAATTTAAAAATTGCTTCATTATGATTTTTAATTGGTTCTTCACCTGAAATATTAGCACTACTACAATATAACGGACCTAACTTTTGAATTAATTTTAGTATATAAGGGCTATTTGGCATACGATAAGAAATACCATTTTTAATGATAGTGACAGAACCTGGTCAAAAAAGATCTAAAAATTGCTTTTGTTCTACAGTTAAATCACCAAGTAATTTGTAATCGGCTACAAAGCGGATGATTTTCTTATTTCGATCACGTTGCTTAATTTCATAAATTACATCTTGATTTTTTGCTAATAAACCAATGATAGTATCAGTAGGGATTAGAACACATTTATTGGCCATTAAAGCATCATAAATCACATTTAAATTAGTTATTCTATAAATTTTCATATTTAATATTACTTATTATTAAAATTCTACATAGATTATTATAATCATTTATAAAGTAGTAAAAAAGAGGAAATTCATTTTTTTTAATAACTTTTTCTAAAATTTCTTTTTGATCAAAACCAATTTCAAAAATAATAGTGAATTTCTCATTAGCAATTGATTTAATGTTATTTATTATAAATAAATAAAAACTAATTCCATCTTTGTGATCGAAATCAATAAGTGCATTAATGGGGTCATAATCTAAGACTTCACGATTTAATTCATAATTACTTTTGATATATGGGGGATTACAGATAATTAAATCAAACCGTTTTTTTGTGTTTAAAAAGTATTCTTGCATACTTTTATGAATAAAATTTACATCTGTTAAGTTATGATATTTAGCATTTTTTTTGCAATTATTAATTGCATCAATAGAAATATCTAATAATGAAACATTCAATTGATTAAAATTCATTTTTGTACTTAACCCTAAAACACCACTACCACAACAAACATCAATAACATTTTTAATTTTATAAGTGGATTGTTGAATTACTTTATTAACAACATCTATTAATAATTCAGTTTCAACACGAAAAGCAAAAACATTTTTATCAACATAAAAGCGCTTTGCACAAAAATAGCCATATCCTAAAATTCTCACTAATGGTTTTCCATTAATAAATTCATCTAATAGTTTAAAATATGTATATTCTAAATTAGAATTTACAATTGTTAAGCGGTTATTTGAAAAACTATAAGAATCGTTAATTTTATTATCTAATCCGTACAGCAACTGAAATGCTACTTGTGGATTACGTAATTTTTTTTCAAGTAGTGAGTATGCTTGAAAAATTAATTGATGGTAGGTCATTAAATATCTAAATTAGCCATTAAACCAGTTTGTTCGTCAGCAATTAATGCATCAATAATCTCATCTAACTCACCCATCATGATTTGGTCTAATTTATTTAATGTTAGGTTAATACGATGGTCAGTAACGCGGTTCTGTGGATAATTGTAAGTACGGATTTTTTCAGAACGATCCCCTGTACCAACTTGATTTTTTCTTAAATTAGCAAATTCTGCATTTTGCTGTTCTTGAGCTGCTTCTCATAATTTAGCTCTTAACATTTTCATAGCTGTTTCTCGGTTTTCAATTTGTGATTTTCCCTCTTGACATGCAGCAACAACCCCTGTTGGAATATGCGTAATTCTTACAGCTGATTCTGTTCTATTAACATGCTGTCCTCCAGCCCCACTTGCACGGTAAGTATCAATTCTTAATTCTGTTGGATTAATTACAACATCAACTTCATCTTGTTCTGGAAGAACAGCAACTGTAATTGTTGACGTATGTACTCTTCCTTTAGATTCAGTAGCAGGTACACGTTGTACTCGATGAACTCCTGACTCAAATTTCATTCGTGAATAAACTTCTTCTCCAGAAATCATAAAAGAAATAAAACTAAAACCAACAGCATTCGGAGTCATTTCTATAATTTTCATTTTTCAATTATTATTTTCAGCATAAGCACGATATGTATCAAACAAATTGCCGACAAAAATTGATGATTCATCCCCTCCAGCAGCAGGGCGCATTTCAACAATAACATTTTTATCATCATTAGGATCAGTTGGCAACAGCATAATTTTTAAATCATTTTCAATTATTGGAATATTAATTTTAGCTTCATCTAAATCCATCTGTGCTAATTCGATTAATTCTAAATCTTTTTCTGTATTTAATATTTTTTCAGCAGCTATACCAGTATCCAATTTTTGTTTATATTTTGCAAAGGCTTCAGCAATTTTAGTAGTTTTTTTTAACTGTATATTAATCTCTTTAATTTTTTTAAAGTCTGTTACAACCGTTTCTAATTCTTTTTTTAAAGCGTCATTTTTAATAGCGACTCGTTCAATTGCTTCATAAAGTTTTTTGTTGTATTCCATAAGTCCCTCAGTTTTAAATATTTTAGTAAAAATAAAATAACAGGTAATCTAATTGCCTGTTATTTAATCTACTAATTTTTAATTATACAAAAAGAATTAAACAATCTTGGCAATTAATGACAAGATGATTAATTATAATTCGTTTAAACTTTTTCTTGGTTCAGATTTACCTTGTATTTTTTTCTTTTCTGGTTTTTTATTAATGTTATTTAAACCAGCTTCAAAACGATTATTTAGTTTTTCAGCACGACCACGTAATGTTGTAGTTGCGTTTTGTTTACCAATAAAAAATGTATGGCAATTTGAACAAACTTCAATTGCAACTTCTGTATTTTTTGCTGCAGAATCAATTATAAATTCTTTTTTACATGTTACACAATTATAAACGCATGGTTTGCTTACTGGATGAATATCTCTCATAATTCTCTTCCTTTATTACAATATATAAGTTAAATAATAAAAAATTAGTAATTTTATGTTTTGAGTTCAAAAACACGAAAATACACTATATATAAATATATAATATTTTAATAAGTTTTGATTACTTTTCTTTAATAAAATTAAAATTTATTGATAATTAGATTAATTTGTAGTGCTTCTTGACTATTTTCATCATCAAGAATTTTTTGAACTTTACTAATACTATGACTAATTGTAGTGTGTTCTCGTCCGTTTAGTAATTTACCAATATCTGTTAAAGTTAAATTTAGTTTATTTTTTAGAATATAACAAACAATATCACGCGCTCAAACTATATTATTTTTTCTAATAGAAGATTTAATTAAATCTGTTTTTATATTTAATTCTTTTGCAACAATTTCAATTATTTGTTCAGGTTTAAATTGTTTTTTAACAACAATATTTAGTGGAATTTCGTTTTCAACTGAACACTCAATTACATAATCTTTTGTGATTATTAAATCTTTATTAGTATCATTGTCCGCTCAAAAACAAATTTGTTTTAGATTACCAATCATCTGTCTAATATCAGTGTGAAAACCTTCAGCAATATATTCTTTAGCTTCGTCAGTAATTTGATATTTTTTTTTGAATTCAAACCAATTATTAATAATTTTAATAGCTTCATTTTTGTCGGGTTGAGAAATTTTACACTGCAAACCTGACGCTAATCTCGAAATAATTCGATTATGAAAACCATTAAGTTCGTTTGGTGTACGGTCAGATGTTAAGATAATTTTTCCACCATTTAATTTTATATCATTAAAGATATTAAAAAACAATTCAGCCGTTGAATCACGTGATTGGATATTTTGAATATCTTCAAAAATTAATAAATCTGCTGAAGCAATTTCTTTTTTAAACTCTTCAACATAACTAGTCCCTTTTAACGAAGCAGAACAAAACTTTCGAAAAAAATCTTGCCCTTCATAATAATAAATTTTTAAATTAGGATTACTATTAGCAAAACGATTACCAGCAGCAGCCACGATATGCGTTTTACCAACACCAACTTTACCAAATAAAAATAAAGGGTTACACATTGAACTATTATCATAGTTATTTGAAAATAAATTTTCAATCGCCTTAATCGCTTTTTGATTAAAATTACTAATTACTAAATTGTTAAAATTGTAACGTAAAAGTTCATCACTAAAATTAAAACGATTTTTAATATTAGTTTCTTTAATTAAAAAATTGCTATTAATTAATAATTTTTCATTGTCTATAATAAATTCAAAATTAACAATATTTTCCTTTTTGAATTCTTCGCTTAGATATTTAGAAATAATGTTAATAAAATTACCGTTAATAATACTTTTTTCAAAGGCATTGGCTACAATTATATATAAAACACCATCGCAAACTTTATGAGTTTTAGCATTTTGTAGAATTGCATAAGCTTGATCAGAAATAAGATCGTATGGAATCCTTTTTATTGCTGAATCATATAAAGTTTGATAATTATTAGCCATTAGTAACCTCTATTTTTTATAAATAATCTTTGTCCTATTATCATATTATATATGTTTATTTTTAAAAAGATATCATTGACTTTTTTAAAAAAATATATTAAATAAAAAATAACTAAAATGTTATAATAATCCATATTATGTTTTCAAAAAAGTAAGAGGGCGGTGGATTTATGAAAAGAACGTTTCAACCTAATAATCGTAAAAGAGCAAAAGTGCATGGTTTTCGTGCACGCATGAAAACAAAAAATGGGCGTAATGTTTTAGCTAGAAGAAGACTTAAAGGGCGTCATAGTCTAACAGTTTCTAGTGAAAAATAATTTTTATGGCTAATTTTATTAGTCTAAAAAAAAATGAAGATATACTTGATACAATTAAGAAACAACAAAAAATTCATTCTAATCAAATCGTAGTTTATTTTCGTAAAACCAATCTAAAAAATGTTCGCTTAGCTATAAGCATTTCTAAAAAAAAGTTTAAATTAGCAACACAACGCAATCGAATTCGACGCCTGATTAAAGCATGGTTTATTGCTGCTGATATTCCCATTAAAAGTTATGATATTGTAGTCTTAGTGAAACCATCGTTTATTGATGGTTCTTTTGTTTTAAATTGTAATAATCTTAAAATAATTCTTCAACGAATTATTAACAAAGAAAAAAGATAAAATTAGCAAGTAATTTTTGAATTGAAAAGGAGGTATAAATGTCATCTGTTGATAAACAAAATTTAACAAATAGAATGCGGATTTCTGTTTCACATTTTGCTGGAGCATCGAATGCAAATTCGATAAAAAAAGAACGTAAGAAAAAAATTTTTAATGTTTTATTAAAAGTTTTTAAAATCATCGTTTATGCATTTTTCTTAGGTATTGGCTTATATGGTTGTTTCCAAAATATGGCAAACCACTGAACAATTAATTCTACTATTGTTGGTAATGGTTTTGAATTAGGATTTCATGTTGATCCTATTCTTGGTGCTAATGACATTCGTTTTGATTTAATTTATTCTGGAACAGGTCCTTGATATCCTATGTCTGATTTTTCATTTGATTATGGTCCTTTCTATGCTTTATTTGTTTGGCCAATTGCACAAATTTTATTACATTTTATGTATGCAACGAGAGATTGACCAGCAGGTTTAAATGCTATTTTAGGATTAATTATCATTTTATTAATTATTCGTGTGATTACAATGTTGATCTCAGCACGTGCAACTATCCAAACAGAAAGAATTTCTGAAATTCAAGGAAAAATTGCAGAAATTAATGCAAAATATAAAGACGCAAAAGATATGCAGTCTCGTCAAAAAAAACAAATGGAAACAAAAGAGTTATATCAAAAACATAACGTTAAACCTTTAGCTCCTTTTGAAAGTATGATTATTACATTGCCGATCTTTTTAATTATTTATCGTGTTGTTACAATTTTAAGACCACTTAAGTTTATTAGTATTTTTTATATTTGAGACTTATCAGCAACACCTATTTCTGAAATTTTTTCAAATTTCACAACTTCAGGATGACCTTATATTTTCTTCTTATTAATTATTATTCCAGTACAAATCTTATCCCAAAAAATACCACAACTATTAGCTAGAAAACGTAATCGTAATGCCACAACTGTTGGTGCTAAAAATAAACAACAGTTAAAACGTGTACGAATGACACAAAACATTATTGCAATTGTTTTAGCTGTTGTAGTAGCAATTTCTGCTTCCGGGATTGGTTTATACTGATTCTTCAATGCGATCTTTACTATTCTGCAATCGTATATCATTCATGTTATTATTATGAAACGCCGTTCAAACAGTGCCACAAGAATTGAATCAAAACTTGCTAAATTAGGAATTAGCTAACTTGAATAAATCATTCATTAAAAATAAACTCAAGCAAGAAAGTTTTGTTCCATCAAAAAAAATGGGGCAAAACTTCTTATTGTCTAATAACATAAAAAATAAAATTGTAGATGTGGCGAATATTAATAAAGATGATCTTATATTAGAAATTGGTCCAGGGTGAGGAGCAATTACTGAAATTCTTGTACAAAAAACAAATATTTTAATTGCTATTGAATTAGATAAAAGACTATATGCACACTTAAAAACATACATAAAAACAAGTAATTTTCATATAATAAATAATGATGTATTATGTGTAGATTTAGATAATTTGATTTTAGATTACAATAATACTCAAAAAATTCAAAAAATTAAAGTTGTTGCAAATCTTCCATATGCAATATCTTCAAAGATAGTTTTAAAAATTATTCAATCAAAATTAATTAATGATGCTTATATTATGGTTCAAAAAGAAATGGCTGAACGAATTGGTGCTAAAGTAAATACACGTGGTTATAATGCTTTTACTGTTTTAGTACAATTATTCTGTAAAACAAAAATTCTATTCGAAGTTAATGCTAAAGAATTTCATCCCCAACCTAAAGTTCAAAGTGCTGTCATTCATCTTGAAAATCTTCATAATAGTGTGAATTTTAATATTGAAGAATTAGGTAAATTTTTACGGATTTGTTTTTTAAATAAACGAAAAAAGTTAAAAAATAACTTATCTAATATTTATGATATTAAAATAATTAATCAAATGTTTATTGATTATAATTTAGATATGAATTTACGAGCTGAAAACATTGAACCAAAAATGTTTCTAAAACTCTTTAATTATTTGAATAGATAAGATAATTAATAAAATTAAGTTGTAGGTCAAATTATGAAAGTAAAGTCATTCGCAAAAATTGATTTAGGATATAGTGTTTATAAAAAACAAAAGAATTTTACAAAACACGATTTTGAATCAATTTTTATACTTGTTGAAAATATTTATGACGATATTGAAATTACTAAAATAGAAAAAAATATTGATGATGTTCATTACTACAATGAAACCAATGAAATTTATGTTTATAGCCGTTTAGTTCATAAGACATTAGAATGGATTCGTCATACTTATCATATTAAAAATCATTATCGAATTAATATTAAAAAAAGAATTCCAATTGGTGCAGGCTTAGGTGGTGGTTCATCAAACGCTGCAGCCATTATGAAATATATTCTAGAATTTGAGGGTATTAAAGAAATTAATTATAAAGATGTTGTTAATAAATTAGGTGCTGATATTCCCTTTTTTTTATCAGGCTATAAAACAGCTTATATAAGTGATTACGGAAGTGTTTTAGAGGATTTGACTGGTCAATTTAAATTAAATTATGAAGTTTATTTAATGAATGTTAATGTAAATACAAAAATTGTTTTTGAAAAATTTGATGATAATTCATGACATGTTATTAAAAATAATTTTAAAACAATTATTAAAAATTTAAAAGAAAATATTGTAGTTAACATACATAATGATCTACAAGAATACTGTTTTGAGCTATACCCTAATATTAAATACAAATATAATGAATTATTATCCGATGGTTTTTATACAATTTTAAGTGGTGCAGGTAGTTCATTTATTCGCATAAAATTAAAAAATAAGGAAGATTTAATTATTAATGAAAATTAGAACACGTTATGCTCCATCACCAACCGGTTATTTACATATTGGAGGAGCGCGAACAGCTTTATTTAATTATTTACTAGCAAAAGCTTATGGTGGGGATTTTATTATTCGTATTGAAGATACTGATATTGAAAGAAATGTGGAAGGGGGAATTAATTCCCAACTAGATTTTTTAGCATGAATGGGTATTATTCCAGACGAATCAATTCGTAATCCTAAAGCTTTTGGCCCATATATCCAATCTGAAAAATTAAAACATTATGAAAAATTAGCATTAGATCTAGTTGATCAAAAAAAAGCTTATTTTTGTTTTTGTAGTAAAGAACAATTAGATGCAGATCGCGAATTAGCTGAAAAATCACATCAAACACCTAAATACAAACGGCACTGTTTAAATTTAGATAAAAAAACAATTGAAAGTAACTTATTACAAAATAAAGAATACACAATTCGTTTAAAAATTAATGAAAATATGGAATATAGCTGGGATGATTTAATTCGGGGTAAAATTAGTATTCCAGGTAGTGCTTTAACTGATCCTGTCATTTTAAAATCAAATAAGATTGCAATGTATAATTTTGCAGTTGTAATTGACGATTATGAAATGCAAATTTCACATGTCATTCGTGGAGAAGAGCATATTTCTAATACACCTTATCAATTAGCGATTGCTCAGGCCTTAAATTATGATATAACAAAAATTAAATATGGTCATTTGTCAATTATTGTTGATGAGACAGGAAAAAAATTATCAAAGCGAAATTTATCTTTAAAACAGTTTGTGTCTGATTATGAAAAAGATGGTTATTGGCCACATGCTATTACAAACTTTGTTGCATTATTAGGTTGAAGCCCAAAAAATAACGATGAAATAATGAGTTTAGAAACGATGATTAAAAATTTTGATATCAATAATTTATCAAAATCCCCCGCCTTTTTTGATATCAATAAAATGAATTGGTTTTCAACACAATATTTTAATAATATTACCCAAGAAGAATTTATCAATTTTATAAAGAAACATTCATTAACTAAAGAATTAGTTTTGAATGATTATACTTTTATTAATAAATGTCTATTATTTAAATCTCATATTATTAATTTAAAACAACTAATTGATTTAGTAATTGAACAATTTAATTGTGATAAAAAAGTATTAGCATCAGATGTAGATTATATTAAAAAAAACCAATTAATAACTGTTGTTCGTGTTTTTTATGAACAATTAATAATTAATGATGAATTTAATGAAGAATTTATAAAAGAAATAATTAAAAAAGTTCAAATAATAACTAATAACAAAGGTGCTAATTTATACATGCCAATTCGTATTGCAACTACATTTAGTTCGCATGGTCCAGAATTAGCTAAAACAATTTGTTATTTAGGACGCGAAAAAGTATTAAAAAATTTAATAAATATTTTAAAAATTTTGGATTAATAATTATTATGGTAAAAAATTTTTTTATAAAAGATCCTATTCATGGTTTAATTACGTTTGATGAACAATATGAATGAGCTTATGAATTAATTAAAACAAAAGAGTTCTGGCGTTTAAATAATATAAAGCAATTAGGATCAAGTTGATCAATTTTCCCAAGTGCTACTCATACACGTTTTACTCATTGTATTGGTGTTTTCCAAGTTACAAGAAAACTTTTAGATTCATTAAAATTCATTAAATCTGATACACACGATTTAATAAATAATAAAAAGATTGTTTGTGCTGCAGCCTTATTACATGATATTGGTCATGGTCCTTTTTCCCATACTTTTGAATATGCAACAAATTATAATCATGAAAAAATTGGTCAAATGATTATTACTAATCCTAAAAGTGAAATTAATAAAATTTTAATTAAACATAATATCAATCCAGTTGATGTTGCTAATATAATTGATCATAAATCCAAAAAAATGTGAATGCAACAGGTTATTTCATCTCAAATTGATGCAGATCGTTTAGATTATTTGCCACGTGATTCTCATTTTACAGGAACCAAGTATGGAAATATAGACTATGATAATATCTTTAAAAAGGCAATAGTTTTTAACGATCAATTATGTTTTTCAAAACACTCTTGAAACGCTTTAGAAAACATTTTAATTTCTCGTTGGAGTATGTTTGAAAATGTTTATTTAAATAAAACACCAACAACCTATGATATTTTAATGAAAAAGACTTTAGCAAGAGTGCATTTTTTATACCAAAAACAAAAAAAATATATATTCAAAAATTCACAATTATTAACAATTTTAAAACCATGATTAGATAATGAATCTTGAGATATTACTACTGTTCAAAATTTAGATGATTATTCATTTACAGCTTTAATGCGATCGTTTGCTAATGAACATGATTTTATTTTAAAAACTTTAGCAACAAATTGGTTACAAAATAAAGATTTTGTAACTTTTGAATATGAATATGGTAAAGATAAATCAAAAGATCAAAAGATTATTCATAAAAAATATTTTTATGAAACTATTACTTCAAATAAACAAATTTATGATTTTAATGAACCGGTTTTTTTATATACAGAAAACAAAAATAATGATATTAAATTATTAAAACTTGAAGATAAGAGTTTAATAATTGCTGCTTTAAAATCAATTAAACAACCACGAATAAAAAAATGAATTTTTTACTATAAACCTTTAAAATAAATACTAATATATTAAATACAAAAAAACTAAAATATAAGGAGTTTAATATGAGCCGTCAATTAGTTGATATTGCTTATAATGCAATAAAAAATAATAAAATTTATAACAAAAAACCATTTACTTTTGATGATATTATTAATGAAATTGTCAAGAATAGTAATGTAAATTTAGTAGAAGTTAATTCACAATTAGGTGTTTTATATACAACTTTAATACAAGATACACGTTTTATTTCAATTGGAGATTTAGAATGAAATTTACGTGAACGATTATCACTAGATGAAATTACAAAAATTAATAACGCTATGTATGAAGTTGGTTTATACAAAGATAGTGACCGTGAAGAAGATGAACATGAAATTGTTAAAAATGATAAATTAACACAATCAAAAGAACAAGAGGAAAGTGATGAAGTAAGCTCTTTATCTGATTTTGTTGGCTACGAAGATGAAGATGAAGAATTTAAAAATAATAATACTTCTAATAAAGATGAAATAGAAGACGAAGAGGAATTAGAAGAGGAAGAATAAAAAAATGTCACCATTAGTTAATGCCAAAAAAATGATTCAAAATGCTTATGAAAATCATTATGCAGTGGCTGCAATTAATATCAATAATTTAGAATGAATTAAAGCAGCATTATTAGCAGCTCAAGAAACAAACTCTCCTCTTTTATTAGCGACATCTGAAGGTGCCGTAAAATATATGGGTGGTTATGATAATTGTTATGCAATGGTTGTTAATTTAATAAAACAAATGAATATTAAAACTCCTGTTTGTTTGCATTTAGATCATGGTACATATGAAGGATGTATAAAAGCTATTAATGCTGGTTATTCCTCAATTATGTATGATGGATCAAAACTTTCGATCGAAGAAAACATTAAAAACACTAAAGAACTTTTGACAATAACTAAATTAAAAGGTGTATCAGTAGAAGTTGAAGTTGGTTCAATTGGTGGGACAGAAGATGGGATTACTTCAGAAGGTGAATTAGCTAATATCGACGATTGTTATCAAATGTGTTTATTAGATATTGATATGTTAGCGTGTGGTATTGGAAATATTCACGGAATATATCCTAAAAATTGAAAAGGTTTAAATTTTAATTTATTAAAAGAAATTAACAATAAAGTGAATAAACCAATTGTTTTACACGGAGGTTCTGGTATTAGTGATGAACAAATTTTAAAAGCAATTAATTTGGGTGTAGCAAAAATTAATATTAATACAGAATGTCAAATTGCATTTTCAAATGCATTACAAGATCACTTAACAAAAGCAGGTGATTTAATTCTTTCTAAACAGTATGATCCACGAAAAATTTTGGCTTATGGTGTGGACGCAATTAAGAATACAATCATTGATAAATTCACTAAGTTTAATAGTTTAAATAAAATAAAATAAGGAGAATAAAATGTCGTTATCAGCAGGTCTTGTTGGTTTGCCAAACGTTGGTAAATCCACATTATTTAATACAATTACAAATTCTAGAGTTGAGGCAGCTAATTATCCTTTTGCAACTATCGAACCAAATATTGGAATTGTTAATATTAATGATCCGCGTTTAAAACGATTAGCAAGCTTAGTTGTGCCTGATAAAATTGTGCCGACCACTTTCAAATTTGTTGATATTGCAGGTCTTGTTAAAGGCGCTTCGCAAGGTGAAGGACTAGGAAACCAATTTTTAAATAATATTCGCGATGTTGATGCTATTTGTCATGTCGTTCGCTGTTTTGATGATAAATCTATTACTCATGTTCATAATAAAATTGACGCATTATCTGATATTCATGTTATTAATTATGAATTAATATTATCTGACCTTGATATTATTGAAAAACGAATTAGTCGTATTAAAAAAAAGGCTGATTCTGGCGATAAAGATGCTAGAAGAGAATATCTTATTTTATTTAACATTGAAGAAGCTTTAAAAAAGGGTCAATTAGCATCAAGTGTTAAATTAACCAAAGAAGAATTGAAAATCATTAATTCTTTCAATTTAATAACTTTAAAACCAATTTTATATATTGCAAATATTTCAGAAAATGAGATTTCAAATCCTGATTTAAATAAAGAATATTTAAAAGTAAAAAAGTATGCTCAAGATAATGATGCTAGATGCGTTGCTTTGTCAGCAAAAATTGAATATGAAATTAGCATTTTAGATGAAGAATCAAAATCAATATTTATTCAAGATTTAGGTATTAAAACAACAGGATTAGATGAAATTGTAAAGCAAACCTATGATTTATTAAATTTAAAAACTTATTTTACATATGGAAAACAAGAAGTACGTGCTTGAACATTTATAAACGGAATGAGTGCGCCTGAATGTGCAGGAATTATTCATAGCGATTTTGAAAAAGGTTTTATTAAAGCTGAAATTATGCATTATGATGATTTAATATCATTAGGAAGTGAGCAAGCTGTGAAAGAAGCGGGAAAAATAAGAATGGAGGGAAAGTCATATTTAATGAAAGATGGCGATATTTGCAATTTTAGATTCAATGTGACAAAATAATTTTTTATGGCTAAAGTGAATGCATTTTCAAAAAAAATAGGATGAATAGAATTAATTACTGGCCCAATGTTTGCTGGAAAAACAGCAGAGTTAATTCGTCGCTTACATCGTTTAGAATATGCAGATGTTAAATATTTGGTTTTTAAACCTAAAATTGATACTAGATCTATTAGAAATATCCAATCACGAACAGGAACGTCTTTACCATCAGTTGAAGTAGAAAGCGCACCAGAAATTTTAAATTATATTATGTCAAACAGTTTTAATGATGAAACAAAAGTTATTGGCATTGATGAGGTTCAATTTTTTGATGATCGGATTTGTGAAGTTGCAAATATTTTAGCAGAAAATGGTTTTGTTGTTATTATTTCAGGATTAGATAAAAATTTTAAAGGCGAGCCTTTTGGTCCAATTGCTAAACTTTTTACTTATGCTGATAAAATTACAAAACTAACAGCGATATGTAATGAGTGTGGCGCTGAAGCAACGCATTCATTGCGTAAGATTGATGGTAAACACGCTGACTATAATGATGATATTGTTAAAATTGGCTGTCAGGAATTCTATTCTGCTGTTTGTCGTCATCATCATAAGGTACCTAATCGTCCTTATTTAAATAGTAATTCTGAGGAATTTATTAAATTTTTTAAAAATAAAAAACGAAATAAAAATATATAATTACATTAATAGTTATTTAATATTTAGGTAAATGTTTTGAAAATAGTTTTAGAAGAATTAAAAGATTATTTGATAGGTAAATTATATTTTAAATATAAATTTATTGGAGTTTTTATTAGTATATTTTTAGCTGGTTGATTAATTTCTTTTATCACAAATTTAATTTTAGCTTTAAATTATGGATATAATGAAAAATTATTAAATTATAAAAAATATTTAGATTTTACAATTTTAACATGTATTAGTTTTTTAATGTTATTTATTACAATAATTTATCTTTTATGGATCAATTGTCATAATAGCACAGCAAGATATTTAATTTTAAAAATCAATAAATACCCACCGAAAAAACCGATCAAAAATCTTCCTTTTATTTTTTTTAGATTAGCTTATTATACATTTAATATAAGACAAAAAATTCAATATCAACAAAAACAAATTTATAAATATTTAATATCTTTTAATGAGTATGTGTAGAATTTAACATTATGGACATAATTAATAAAAAAATTAATTGATTTCCTGGGCATATGAAAAAAGCAACAGATGAGATTTTAAAAAATCTTAAAAATGTTGATTTTTTTATTCAATTAGTTGATGCTAGATGTCCATTAACATCAAGTAATATTGAATTATTAAAAGAAGTTTCATCCAAACCAGTTATTAATTTGGCTAATAAGGCCGATTTAAGTGATTGAAAAAAAAATTTCGATAATAATTTTTTATTAATTTCAACTAAAAAAAGAAATGATAAAAATAAGATTATTCAACATTTATATCAATTATTTGATCAAAAAATAAAAATATACCAAAAGAAAGGATTAATAAATCCTAAATTTGTAGGTATGATTATTGGTCTACCAAATATTGGTAAGTCTTCTTTGATTAATTTTTTAGCTCCCAAAAAAGTATTAAAAGTTGAAAATAGACCTGGAGTTACTAAGACTCAATGTATTCGTCAAATCAATCAACATTTTTATTTAATTGATACACCTGGAATTTTTTTAAAAAATATTCAAAATGAGCGTGATAGTTTTGTTTTGACTTTGATTAATTGTGTTAAGAAAGAAGTTTTGGAATTAGAATCAATTGTACATTTTGCATATGATTTTTATTTAAAAAATTATAGGAATGAATTATTTATAAAATATAAAATGAATAAGGTATTAAATTTTGATGATTTTATTCATCATATTTGTGAGTTATATAATTATAAATTAACAAATAATGAATTTGATCATGTAAGAGCTTATGAGAATTTATTTAATGATTTTTCTAATGGATTGATTTGTAAAGTTAATTATGATTAGTAATTTTAAAAATAAATCATTATTGCTAATAATTTAATGAGGTGATACCATTGAATTATTTAACACACTATTTCAGTTTGAAGTATAAAGGGGATTGATCAAAAATTTATTTAGCATTAATAAATCATGAAGATCAAAATGAATATTTATTTTTAGAAAAAGAAGATTACGAAAAAGTTAAAGCTATTGATTTAACTGATGAAAAATATCCTAATAAATATAAAAAAATTAACCAACCTCCTTTTATTTTATATTATTCAACAACAGTTGAATGACTTGATAAAAAAAATATTTGGTTAATAGATTATGAAAATGCAATATCAAATTATGATATACTAACCTTGATAAAAGCAGGATATGGATTTGTTATTTATTATGATAGTGTTGTTTTGGACACACTTATCAATCGTTTAATTTCTCTTAAAGCTAGAATATGTATCATCACAGATGAAGGAATCTATAGTTCTAAAAACATTAAATACTTAAGTTACTGCAATGTTAGTGTAATATCTGAAATTCCAAAAGATTACAATAGTAATAAAATTACACAGGATTTAAAACGTCTTGCATGCGCTTCAAGCGACCATATTTTATGTGTTAATGAGTTATATCAAATAGATGATTATCTTTTTAATAAATATAAAGATTGCCAATTAGATTTGGTGTTATTAGATAAAAAATTAGCTAAGCAAAACACAATTTATCCCAAATACATTAATCGTATTTATCAATTTATTAAAAATTCAAAATTAATAAATTAAACAACAACTATAAAATATTATAATTATTTACAATTGTTAAAACGAGCAAATAATTGTAGTAAATGGGAGAAAAAATGCAAAAAGATTTAATAGTAGTAGAGTCACCAAATAAAATTAAATCAATTCAGTCTTATTTAGGTGATAGTTATAATGTAATAGCTACATGTGGACATTTACGTGAGCTCGCAAAAAAATCTGGTTATGATCCAAAAACTTATGATTTAAAATGAGAAATAATAAAAACAACAAATAAAAAATTATCGAAAAAACAACAAATAAAAACAATTATTGATTTAGCAAAAAAATCAAATAATATCTATTTAGCAACTGACCCTGATCGTGAAGGTGAAGCTATTAGTTGACATGTTTATGATTTATTACCAAAAAAAGATAAAGAACGTGTTAAAAGAATAACTTTTAATGAAATAACACAGAAAGCTATTTTAAAATCTATTGATGAAAAACATGATATTGATTTTAATTTGGTAGATTCGCAATTTGCAAGACGAATTTTAGATCGAGTTATTGGTTATAAACTTTCTGGATTAGTTAAAAAAAACTTACATGGCTTATCTGCTGGGCGAGTACAATCGATCGCTTTATTATTTGTAGTTGAGAGAGAGTTAGAAAGACGTTCTTTTGTTAAAGAAGAATGATATCAAATTGAAGGAATAATTGAAAAAAACATTTTTGTATCTTTAAGAAAAATTCCCTATGATGTTGAATTATATAATCAGAAAGATAATAGCGATAGTGATTTAAAATTTCTACACAAAAAAGATGCACAGCGTGTGATTGATGATTTAAGTGATATTTTTAAAGTTTATAGTATTGATGATTTGAAAATCACCAAAGGCGATGTTCAATTACCATTAACAACTGATAAATTATTACAAATCGCATCCAATAATTTTGGTTGAAGTGCTAATAAAACAACTTTAATAGCTCAACAAATGTTTGAAGGATTAGAAATTAATAATCAGCAATTAGCTTTAATAACTTATCCAAGAACAGATAGCGAGCGATTAAGTGATGATTTTTTACAAGAAGCTAATGCCTTTATTGAAAATAATTTTGGTAAAGAATATCTAGAAAATTTTAAAAATAAAAAAATTAATAGAAAAGAAGTAAATATTCAAGACGCACATGAAGCAATTCGCCCTGTTGATATTAATTTAAAACCTGAAGAAATTAAAGATTATGTTTCATTAGATATTTATAAGCTGTATAACTTAGTTTGAAATAAAACACTATCAGCTTTAATGAAAGTGCCAACTTTTAATAAGCAAGTAATTCGTTTTATTAATAATGAATATAAATTTTATACAGCTTATAAAACAATTCGAACTTTAGGATATTGAATTTTAGATTTTTATAAAAAGCAACGTGATGAATTAGAATTCCAACCTCCTATAATTAATGTTGGTGATGAATTTAAAAAAGAAAAGATCGATTTAATACAAAAAGAAACTACGCCACCGCCTTATTACACAGAAGCCTCTTTAATTGCAGCGCTAAAAAATGCCGGAGTAGGTCGGCCTTCAACATATGCAACAATGGCAACTATTGGCACCACTAGGGGTTACATCAATAAAGATAAGAGTAAATTAATTCCAACAGAATTAGGGATTAAAGTCATCCAAGAGTTAAAAGAAGCCTTTCCAAAAGTGATTTCTATTAAATTTACATCTGAGATGGAAGAACGTTTAGATGTTATAGCTAATGGCAAATTATTTTGAAAAGATTTAGTTAGCACTTTTATTCCTATTTTTGAAGAAGAAGTAAAACAAGCATACACTAAAATCGAAAAAGTTCCTGATGAAAAAATTAATCGATTATGTCCTATATGTGCTCATGATTTATTGATTAAAAGATCTAGAATGAACACTCGGTTCATTGCGTGTTCAAATTTCCCTACATGTCGTTTTACAGAATCGTTAGAGAAACCAGAAATTCTTAACGAATTATGTCCTAACTGTTCAAAATCATTGGTTAAACGTAAAAATCGTAAAAATAAATATTTTATTGGATGCACAGGTTATCCTGAATGTAATTTTATTCGTAATTTAGATGAATCAAACAATGATAAAAACGATTTTAATCAATAATAATATAATATTAAAAAGAGGAAAATTTATGTTAATTAAATTAGAAAGTAACCAAAATTTAAATGAATTATTAAAGGAAAATCATTCTAAACCAATATTAATTGATTTTTATGCGGATTGATGCCCTCCATGTCGTATGCTTATTCCAGTATTAGATTCAATTGAAAAAAAACATGGTGATGAATTTACTATTATTAAAATAAATGTTGATCATTTTCCAGAATTATCAACACAATATCAAGTCAAATCAATCCCTGCTTTATTTTACTTAAAAAATGGAGATATTAAAGCGACAAGTTTAGGATTTATTGATGAAAATTCTTTAGTTAATAAATTACGATCAATTTAATAATTTAAATTCATTAGTTTTAACTATTGCATTATTGCTTATTATTTAATATAATAAATAAGTTATATACATAAAGCAATAATTGTTTTGGAAACATACTCAAGTGGTTAAGAGGGCACCCTGCTAAGGTGTTAGGTCGTTCTGCGGCGCGTGGGTTCGAATCCCACTGTTTCCGCCATTAATAATTAAAAATAGGGGAAACCCTATTTTTTATTTATATAAATTATAATTATTAATAAAAAGGACGATAATAAGTATGAAAAAACGTATTTTAGCCAAATTCATCATAACATTTATTTGTGTATTATTAATAATTAATATTTGTTTAATATCAATATATTTTCACCATCGACCAACGGTTATTGTTACAGATATTTTAATATCATTTTATATTTTAAATACTAGTATTGGCTTATATATTTTATCTTCAAGACATCGGCAAGATCGTTCTAAAAAATCATGATTGTTTATTTTTTTATTTCTACCAATTGTGTCAATTATATTCTTTTGGATTTTTGGATCACGCCCATTTAAAGATAAAAAAATTAATGATATCATTACAAACCGTCGTGCACTTTTTAAATTTGAAGATTATGAATTTTATCATAGTTTTCAAAATATGATTATTAATAATAATGGACTTAATTTAATTCGTAAAATTGGTCAGTATAATTACCAACTTTTTCGTACACCTATTTATTGTGATAATCAAATAACAATTATTAAAGAGACAAACGAAATGTATGAACAAACTATTAAATTAATAAGAAAAGCAAAAAAAACAATTTATTTACAATATTATATTATTGAGAATGGTCGTTGATTACAATCTCTTGTTAAAGAATTAATAAAAAAAAGTAATGATGGTATAAAAATATATATATTATATGACCCAATTGGTTCGTTAAATAAATTTCCTAAAAAATATCGTCAACAGTTAATTAATAATGGTATAGAAATTGCTTGTTTTAAAGCACGTGCAGATTATAAATTTAGAAGTACTATTAATTTTCGTTCGCATCGAAAATATTTAATTATTGATGGTGAAGTTGCTATTAGTGGCGGCTCTAATATTGGTGATTCTTATTTAAATATTGATCCTAAGGTTGGTCATTGAGTTGATTTGAATTATATTTTAACCGGAGGAATTGTGCATACAATTCTTTTAGAATTTATTCATGATTGATACTACTATACACCATTTTCAAAAAAGAATAATTTTAATGTTGTTGTTGACAGTATGCATCCTCAACCTTACTTATGTCAAGGAAAAGATGACTTAATAAATAATTCTATTAAAAATAAAACAATAACAACACTTTTGCATACAGGACCAGACCAATCAACAAGTTCTTTAAATGAAATTCTGGTTATGGCAATCGCTAATGCTAAAAAATCAATTACAATTTTTACGCCATATTTATTTCCTACTGAAGATATTTTAATAGGACTTAAAGCTGCAGCGAATACTGGAATAAAAGTTAAAATTATTTTACCTGGAAGGGTTGATTCTTGAAGTTTTATCATATCAATGAATCGTTTAAGTTATGAATCATTAATTAAGTCCGGTGTACAAATTTATGAATATAGTGGTTTTTTACACACTAAAGCGATTTTAATAGATGATGATATTTCACTATTAGGTTCTTATAATCTTGATAATAGAGCATTAGTAATTAATTACGAATCATTATTAACAATTTATTCAATACAAGTAAATCACGACATTAACGAGATTTTTAATCAATATCTCCGTAATTCACAGTTAATTAATTTAGAAGATTTAGATAACAAATTCACATTCAACGAATTAGTTCAAACAGTATTTATTAATATTTTTCAATTACTTTTATAATAAAAGTTATTATATTAATAATATAAGATATCAAGACAATGCTTTAAGTAATATAATTAAAATACATTTAAAAAATGATATTTTATGTAAAGGTTTAATATGATATCGAAAGAATTTACCATCGTAGCAGAATTGTCAGCATTTATTAAGGCGATCACTTATTTTGTTAATGCAGCTAGTGATTTTAGCTCTTCAATTACTATTAAAGCAAATGGGCGTCAAGCAGATGCTAAATCAATTATCAACATTATGGCTTTAGGAATTAAACAAGGCACAAAAATCGAGCTGAGTGCCGTTGGAAATGATGCAAATAAAGCAATTAACAAATTAGAAGAAATTTTAATTGAACAGAAATTAATTTAAATATCTTTATTTAGTGGAGACTATTAAATATGAATCGTTATGTTAACGCAATGCGTTCACTTGCTTTACAGGCTATTAATAAAGCAAAGCAAGGTCACAGTGGAATGAGTATTTCAGCTGCACCTATTGTTTATACCCTTTATAAGGGATTAATGACTATTTCAAAATCACATCCAAAGTGATTTAATCGCGATCGTTTGGTTTTATCAGCTGGTCATGGATCGATGGCATTATATCCTGTTTTTTATTTTTCATCATTATTAACATTAGATGACATTAAAAATTTTCGCAATGATAATTATTTAACACCAGGTCATCCTGAAGTGTTGGCTAATAATTATATTGATGCATCAACAGGACCACTAGGGCAAGGGGTTGCTAATGCAGTTGGTATGGCAATTACTGAAAGTTATTTAAGAACTGAATTTGCTACTCTTAAAGGTGTTATTGATCATTATACTTATTGTATTGTTGGCGATGGGGACCTACAAGAGGGAATTTGTTATGAGGCAATGAGTATTGCTGGAAAATTAAAATTATCGAAATTAATTATTTTACATGATTCAAATGATTACCAATTAGATTCAGCAGTAAGTGATGTTAATATTGAAGATTTAAAAATGCGTGTTGAATCAATGGGTTGAAATTATCTAAAAACAGATAATAATCCTGAAAATATTTTCAAAGCAATTGCTGAAGCTAAATGAAAAAAGAATGTTAAACCAACGTTTATTGAAGTCAAAACAATCATTGGTGAGGGGACATCTTTTGAAAATAGTAATGAAGCGCATGCTGCAGCAATTTCTAATGATGAATTAGAAAAATTTTCTAAGCGTTTTCATATTAAAACAAATAATTTTGAATTTCATCAGGAAATTTTTGATCACTTCTTTTTTAATGTTGTAGCTCGGGGCGAAAGTGCTTATAATCAATGACAACAATTAGTTGACCAATATATGCAAACTAATCCAGAACAAATGCAACGTTTATTAAATTATATTAATGGTAATTATGAAGATTTAAATAAATTATTAGATGAAAATAAAATTACTAATTTAAATGATTCTACTCGCAGTTATTTAAAACAATATTTTAATCAATTAAAAGATTTAAAATCAGCTCTTGTTTTATCTGCTGATTTAGCTAAATCAACTTTCACAAAAATAGGCGAAGGCGCTTTTAACGATAACCATAAAAATCCATATATTAAATTCGGTATTCGTGAATTTGCTATGGCAGGAGCGATGAATGGAATAAGTTTACATCAAGGCGTTAAAGCGATAGGAGGCACTTTTTTAGCTTTTTCTGATTATATGAAACCAGCAATTCGTTTAGCTGCCATTAGTAATTTAGCTAATTTATTTATTTTTAGTCACGATAGTTATGCAGTGGGTGGTGATGGACCTACACATCAACCTGTCGATCAATTACCAATGTTAAGAGCAATTCCAAATGTAGAAGTAATTCGTCCTGCTGATCATTATGAAGTTAAATATGCTCTTTCATATAGTTTTAAACAAAAACAAAAACCGATTTGTTTAATTACTTCACGTCAAACTATTAAACAAATTAATGATCAAAAACCACAAGATTTTAGTAAGGGAGCATATATTATTAATTCGCCTTTTTCTTTTTGTAAAGATGTAGATTACACGATTATTGCTAGTGGTAGTGAAGTTAGTTTAGCAAATGACGCAGCAAAAGAACTTTTTGAAAAACATAAACTTAAAATTAAAGTTATTTCTGCTTTTAACTTAAATTTATTTTTACGTCAAAAACCTGAAGATATTAAGAGCCTTTTAGCTTCAAAAAATGGTCTATTAGCAATTGAAGCATCAAGCGAAATGCTTTGATGAAAATTATCAATTTATACAAATAAATTTATACAAATTGCTGCTAATCAATTTGGGCGTAGTGCAGATGGTGATAAATTAATGAATGAATTTGGTTTTAGTGTTAAAAATATTATTAATCAACTTTTAAACCAATAATAAGGAATATCATGAATAAGTATAATATTTATGTAGATCACACATTATTAAAAGCTGACGCAAGTTTAGAAGAGATTCATAAGTTGTGTGAAGAAGCAGAAGAAAATGAATTTTATTCAGTTTGTATTAATCCTTGTTTTGTTAAAGTTGCAAAACACTATTTATTAGAAACGTCTGTTAAAATTTGCACAGTTATTGGTTTTCCACTAGGCGCTAACACAATTGAAACTAAAGTTTTTGAAACAAAAAACGCTATAGATTTAGGTGCTGATGAAATTGATATGGTTATTAATATTAATCAATTAATCAATGGTAATAGAGAATATTGCTTAAATGAAATTAATCAAATTAAAGAAGTTTGTGGGGATAAAATTTTAAAAGTGATCGTTGAAACAGCATTTTTGAACAGGGAGCAAAAAGAATTTGCAGCTAAAATTATTCTAGAATCAAATGCTGATTTCATTAAAACATCAACTGGTTTTGCAAAAGAAGGTGCAAAACTTGAAGATATTATTCTATGAAAGCGAATTTTAGGTGATGTAAAACAAATTAAAGCATCAGGTGGAATTAAAAATTTTGAGGATTTTAAATCTTTTATTGAAGCTGGAGCTACAAGGATCGGAACGTCATCAGCAATTAAAATATTAAATAATCACTAAAAAACAAAAAGAAGGTTTTGCATCTAAACCTTTTTTTGTTTACTTAATAAATACTGGAAGTAAAATTTAAATTTATTATTCCAAAAATTACACTATAATTAATTATTATATTATTATCGTTATAGAAGAATGGAAGTAGTGATTTTTATTGTCTTATCAAATTGGTTTTAAACAAAAAAAATTGTCTTTTTTGAATTTTAAAAAAATATTTGGCGATAAACAATTTATCAAGAAAGCATTAAAATTATTTTTTCCAGCGATTATCCAAAATATTATCATTACAGCAATTGCTTATGTTGATAATTTTTTTCTAGCAACATATACCCCTCAATCACAAGGATTAGATGCTAAAACAGCAACAATTCTTGCAACTCAATTATTATATTTACCAACAATTTTTACCGTTGCTGTTTGTTATGCATGCAGTGTTTATAGCTCACAATATTTAGGAAATAAAAATTATCATAAATTTAAAGAAACAACAAATTACATGATTTTAGGATCTGTTATATTAAATATCCCTTTTTTAATTATTTATTTAACTTTTCCACATTGATCTATAACAATATATGCAGGATCAATTAATAATTTTAGTAAAGATATGTCAATGGTCACATCTAATGAGTTAACTAATTTTAAAAAAGTTAATGAATGAGCATCTATGTATTTACAATACGCAATGATTGGACAAGTTTTTTATGCTTTTTCATATCCTTTTGCAATTGCACACCGTCAAGATGCACGTCCAGCAATTCCTTTATTAGGAAGTATTGCCGCACTTTGTCTAAATTGTATTTTAGATCCAGTATTAATGATTTTAGTTGCCAAAACTCCTAAACAAGCGATATTACTTGCAGCATTCTCAACAATCGCTTCTCGTGGTTTTGATTTATTAATTGTTTTGCTTTGTGTTTGTATCTTTAAAAAAAGGGCAACATATGTTATTAATTATTGAAATTTAACATGAAGATTATTAAAAAATATTTTTAAACGCGGGTGGTTCATTGCTATTAATGAAATTATATTTTCAATTTCAGTAATCTTATTAACACTATTTTTATATAAATATCATCCTGACATTCGCTCTTCTATCTCAACAGCTTTATTAATAGCTCAATTTACCAAACTAATTTGACCAGGAATGAACACTATTGTTGCAGTTCTTGTTCTTTCTTATTTAGGAAAAAACGAAATTAATACTGCTAAACAAAATTCACGATATACAGTCGCTTGATCACTTATTATAGCTAGTATTTTAGCAATTATATTATTTATATTATCTTTATTTATTAATCCTTTATTAAACCCTCCATTATCAAATTCGTTATTAGATATTAAAGATGCTAAGAAAGTGGCAAAATTAGCTACTCAATTAGAATGAGTTTTAGTTGTTGAAATTATTATTTTAGGGCCATATGCTGCTATGAATAATTGCATTCGTGGAGCAGGAGTTATTTATGGAGCAATTAATGATACTATGGTGATGATTATATGAGTAATGATGTTTGCTTTAATTAGTACATTTGCTTATAATCCTAAAATCCCACATCTACTAACATTTGCTTTGCTTGAATCAAATTGATTATTAAAGATGATTCTTGTAAGCATATTGTTTACAAAGGTAAATTGAGCTAAAAACTTAATAGGTAGTAATCAATTTGAAATAAGAAAAAACAGTTTTAAATTTAGATTTAATTACAATAAAAAATAAATTAAATATTATCTTAATTTCAATTGAAGGAAGATTATATATTTTTATAAAATATAAAAATTAACCATCTTTTCTTTTAAAAAGGTCGTTAATTTTTGCTATCATTTTTATATACTAGTGTCATTCTTTTAAAATTTTTTATTTATATTATTGAAACGTTATATTTAAAAACATTTCTGCTTGATTTAAGGATGAACGTGGTTGTAATATAATAAGTTTTCAAACAAATGATACTTTTTTGATTATAGGTGCTTATTTATAATTATTCTTAATTTACCACCAAAAAGACTATCACATTTCAAATATTATAATGAGTTTTTCGTGATAATCTTTTAAAAATTAATTCAAAACATTTAATTAAATTAAAAAATTGATTGTTTAAAAAGTTACTATAGATTATTTATTGAAGCTAAATAAAAACTCATTCATCTAACTTTTAGAAAGCAACTAAAAGTATTTTAAAGGAGAAACATATGCGCTTCGATTTTTTTAAAAAATCACAGAAAATAAAACCAAATTGGTTTATAAATCAATTTGGTGATAAAATTTTCATGAAAACAGCTGCTAAATTATTTTTCCCAGCAATTCTTGAAGTTATCATTTTATCGTCTGTTAATTATTTTGATAGTCTTTTCATTGCTTTATTTACACCTGAGAATATGGGGACAGCAGCCAAAACTGCAACCGTGTTAGCGACACAACTAATGTTTTTGCCAACTATTTTTTTCTATTCTATTGTAGCAGCAGCGGGGATTTTAGCCGCTCAATATTATGGTAAAAAAGACTATTTAAAATTTAAAGAAACAATTAACTTTATGTTGTTGTTTAGTTTTATTGTTGTAATGATATTTATTGTTATTTATATGGCAGCACCATTACAAATGATTCAATTATATTCAGGAAATAATTTAGATGTTTCAGCTTCAGAAGAAGCAAAACGAATTTATGATTTAACAAATCATTGAGCAGCTAGTTATTTACGTTGACAATCATTAACTTTAATTCCTTACATGTTTTCATTTTCATTAGCCACTGCTTATCGTCAACATAATAATGCAATCATGCCTTTAATTTCAAGTTCAATTGCAGTAACTGTTAATGTTATTTTAGATCCAATTTTGATTAAATATTGTGCCGTAACACCATTTGAGGCAATTTTATTTGTGGCTATTGCTACAATTGTTGCAAGATCTATTGATGCTTTAATAATGTTAATACTAACATTGTTTCGTAAACAATACCCTTATTATTTTTTAAATTATTTGAAATTAAGCAAAAAGGTCGTTAAATTAATTTTTATTCATGGTTGACAAGTTTTACTAAATGAAATTTTATTTTCAGTTGGAACAACAATTATATTAATGTTTTATACAAGATATAGTCAAGATCATCGTGATGCTATTTCAACTGTTGCTTTAATTATTCAATTTACTAACTTAATTTGGCCAGGTGCCGCATCAACAGTTGCTGTTTTAGTGTTAGCTAATTTAGGTGCTAACAAGCATGATTTAGCTAAGGAAAACACGCGCAAATTAATTAATTGGGGTATGATAGTTGGAATTAGTTTGGGAATTATTTTGTTAGTTTTATCATCGTTTGTAAATAAATTATTAAATCCACCGTTGGATTATACAGAACAAGGTTTAAAACATGCAGCATACACTGCGACAATTGCTATGTATTTAGAATGAATTGTAATTATGGTAGTATTGACACAAGGACCATATTCAGTTATTTACTTCTGCATTCGTGGTGGTGGTAGTCGTTGATTATTAACAATCGACTGTTTATCAACTTTTATTTGATTGATTATTATGGGATGTATAACACATATAAATGTTCCAGAAGATTATAATGATCGTTTACATGTTGTATTATTATTTTTTATCATGGAATCCCAAAATATTTCTAAATTAATTATGGCTATTATTATCTTTAAAAAGACAAAATGAGCTCAAAATTTAGTTGATGAAGAAAAAGAAATTACAAACGAAATTAATGATGATAAAAAAGAGAAAAATAAGCAAATAATAAAATTAGAAAACAAATTAATGGACACTGATATTGAGGCACGATAAATGAAATTTAATAGTAATTATCAAAAATGAATTCTTGATTCATTAATTAACCAAAAAATTTTCGAACCAACACCTATTCAATTAAAAACGATGCCATTAATCGCTAAAAGAGAAAATATTATTGGCGTTGCACCAACAGGGACAGGGAAAACTTTAGCCTTTGTTTTGCCTATATTAAACAACTTAGATTTATCTCAAAAACTGCAAGTTATAATTATTACTCCCACTCGTGAGTTAGCTCGCCAAATTTTTAGTAAAATTATTGTTTTTAAAAAACATCAACCACTTTTACAAGTTAAAATGTTAATTGGTGGTGAAAGTATTGACCAGCAAATTAATTCACAATTAAATAAAAGCCAACTGCTTATTGCAACTCCAACGCGTCTAAAACAAATTTTAACACGACAAATTCTTGATTTACACCATGTTAATGCACTTGTTTTTGATGAAGCTGATATGTTAATGGATCTTGGTTTTATTAGTGATTTAGAGTTTATTAACTCACAATTTACAAATAAAGATGTACAGAAAATAAGTTTTAGCGCTACACTGCACGAAATGTTATCGATCCAATTAAAAAAATATTTTAAAAATACAAAGATTATTAATATTAGTAATTCAATTTATGAAAATAAAAAAATAACCCATTATGTTATTCATAATCACGACAAATGACATTCATTATCTATAATTATGAAGACTATTAAACCTTATTTATGTATCATTTTTGCAAATACAAAAAAAGAAGTTGAAGCAATTTATCAATATTTAATTCAACAAAATAAAAATGTTTGCATTTTGCATGGCTCACTACTTCAACGTGAACGAAAAAACAACTATCGTGATATTAAAAATAATAAATTTCAGTATGTTGTTGCCTCAGATATTGCAAGTCGTGGTTTAGATATTGATGGTGTAAGTCATATTATTAATTGAAATTTGCCTACTGAAAATGAATGATACATTCACCGTGCAGGACGTAGTGGTCGTGGTAAATATAGTGGAGAATCATATGTTTTATATGATCAAAAAGAACATGATAAGTTATTGAATTTATCAAAAAAAGGAATCATCTTTCACCATAAATTCATTAAAGAAAATGAGTTATTTGATAAAGAATTTCTTTTTAAGAAAAAAAATCAATTTAAAGATTTAAAAACACAAAATGAAATTCAAAAAATAATCTCAACAACAAGTACAAAAATAAAACCAGGATATAAAAAGAAAATTAAAGAAGCTATTCAAAAAATTAATCAAAAAAACAAACGCTCTCATATCGAAAAGAAAATGAATGCTCACCGAATTCGTGGATATAAATTAAAAAATAGTTAATAAATTTTAAAAAAATAATATAATAGTAATGCATATAAGTTTTAAAGGCTCGTAAAACTTATATGCATCGACTTTTGTTTATGATAATAAACATTTCGATAATTAATTACGTTAATATATTTTAGTCTCTATTTTAACAAAAAACTTTTTTCTTTAGAATTCTCATTTATTGTTATAAATCTTTTAATTTAGTTTGAAGATTTAAAAACAAACAAGATAAAAACTATTTATATTTCATAAATACCTTTATTTTTTATTATTATAATAAGTATTTAACTATAAGATTTTAAAAATTTTATTTTTAAAATCAATATTCTTTTTTACAATTCCTTACAAAGTTCTTTTTTAAATAATAAATACAAGTTGTAGTATTCCATTTTTCCCTTTTCAATTAATTACTTTCATATAAATAGGATGAGTTTTTCTAAAAACATTTTAATAAAAAACACCTTACAAACAAAAATGTAAAGTGTTTTTTTATTTTTATTAGTTTTCTAACTTATCACTTTCTTCATCATAAAGTTGAATATCATTAAAATTTAAATCTAGTGTGTTAATTCGGCCAAAAAATTCAAATTCTACAGTGGCAATTTTCTTGTTTTGATCAATTTTTGCAATTTTTGCAATTTCACCAAAAAAAGTATCTGCGATAATTTTAACTTCTTGATCAACTTCAAAGTTCGTAAAATTTTCGTCTTCATGCAATTCAATAACTTCAATATTTTCTTCATGGTCAACTTTTTCATTTAGACTTATTAAAGCACGTTTAGCATCATTATCAGCAATTAATTTTAAGATCTTATCTTCAGGTACAGGAATTGGTTTAACATTTTTTCCAGATGAACCAACTAAACCAGTAACCATTTGGGTATTACGAATTAAAAACCAAATTTGATCGGTCATTTCCGCTTTTAAAAAGATATATCCATTAAATTTATTACCTTCTTTTATTTTTGTACAACGATATTTTGTTGCGCCATCAACAACTATTGTTTCTCATTTTGTGTTTGCACGATTCTTCATTGACCGTGGCGCTTCTGATGGCTCATAAATTTTAACTTCTTTAATTTTTTCTTTAATAATTTTTAAATCTGATAATTTATCACCATAACCATAGCCATTTAATTTATCTTTAATATTTTCAATAACTTTTTGTTCATTACCGCTTACTACCGTAACAATGTATCATTGATGTCTGTGATTAAAATCAATTTTTAAATCACTTAATAATTTAGAATCTAAATCTTTAATCTTATATGCCATAATCTTTTCCTTTACTTTTAAAATTTTATTTTGCTGATAATTTATAAAAACCTGTATCAATACCAAAAAATATTGCAGCAAGAATTAAAACAATAACAATTACAATGATTAAACTGATAATAACAGAACGTTTAGAAGCCCAAGTAGTACGTCTGATTTCTTTATTAATGCCATAGAGCCAACGTTTTAATTTAAAACTTATTTTTTCAAAATCTACACTATATTGTTCTAATAATTCATAATATTCATCAACCATTTGATCAATTGTATTTTTTAATTCTAAACGGTGGGTATTAAATTGTGTTTTTGAAATTGATTTATCTTCTTTTAGTAGTCTTAAAATAAGTTTTTGATCATTTTTAGCTTTTTTAATTAATTTTTTATTAAGATCTAATTGTTGATATGCTTGTTGTGCCAAAATCTTTTTTTGTTGTTTACGTTCTTTTTCTAATAAAAGATTACGTTCATATTCTTGATCTTGGTACGTTAAATATTTATTTGATTTTTGTTTTTTGTCATTAACCATTATTTATCTTGTTTCCTTATGCAGTGTTTTCATATTGCATTTTTTACAATATTTATTCAATTCAAGTCGTGAGATAGCAAATTTATTACGTGTTGTTTGATAATTTCGACTCATGCACATTTCGCAAACTAGTATTATTTTTTTAATCATAATATTCAATTATACCTCATTAATAAATATTAAATCAATAAATCATTTAAACAAAAACTTTTTGCTGTTTGTTTACTTTTTTATGATTAAGATTTAAAATAGCATCATTTAAAAATGGAAATCCTATGAAAAATATAAGCATAACAAAAAATAACAAACTAACGATTCATGGAATGACACTAACATTATTTGCATAAAATAATCCAAAAGTATATAGTTTTAAATTATAAGGATCTAATCAAGCATAAATTGTATATCCATATAAAAATTGGTATAAAAAAACAAATAAACAACTTGTGATGCCAATTATTCAAACACTTAAAATTCAATTATTAGCAATTTTTAATTCCTTATTAAGAATATTCTTATTTTTTAAACTACTATTTTGATAATTAAAAAATCATTTAGGACGTTTTGTAACATCACTTGTATAATATTGTTTTTTAATGCCTTTTATTTTTTGCTTTGTTTTAACTTTTTTATAAAAGGCAAAAGCAATTATTAAACCATAAACTGCAAAAAAGAAAACAATTGGATAATTAGATAATAAATCAATATACGCGTCTGTATTTAATGGAATACTAATTATCATAAAAATGGTTATTCATGTAAAAAAAATGAATAAAACACTGATTAATCCTTGTGCTTCTTTAAAATGTTTTGATTGAACACGTTGGATTCAAGATCATCAAAAAATAATTTTTTCATTAATTACATATTGAGCATTATGAATTGAAAAAATAACAAATGAATTTAAAACACCAATAATTGAAATTGTAATAAAAACATTAACAATAATAGTAATAATTTTTAGGAGATTGATATTATTTTTAAATACTTTATTAATTACACCATAAGCATAACCTTCGCCTACAAAAACTTGCCCTATAGTTATTAGAATATAAACAATAGCGACTAAAAACATACCAACAATTAATGTAATTGGAACATTTTTTTGAGCATTTTTAATTTTGTGTTGTAATGATGAAATGCCTAAAAAAGAATCGAATGAGAATAAGATCGAAGGAAGAGAAGCTAATATTCCTAAAAGACTAAAATTCGCATGCTTAGGATTATTATGATTGAACAATGAATTTTGTGCATTGTTGATACCATATGTAATACCAATAATAGCAATAGCAATAATAGGAATAAATTTAATAATTGTTGCAATGTTTTGTAAAATTGAACTAAATTTAAATGAAATAAAATTAAGCAACAAAAAAAATAAAAACAACCCGATAGCCAAAATAAAAATTTGATATATTTGTGGTGATGATGAATTTTTAGGTATTATTGTCATTAATAATGATTCTGCAGCGAAAAAGGAAATGGAAGGCATCAGTATTCCATTATAAAAAAAAGTTTGCAAAACAGAAATAAAACGAGCACATTTAGGAGCTTTTAATTCATCTATTATACCAGCTATTCCAGAACCAGATTTTTTAGAAAAGGTAATATATGCAAAACTTAATGCTGTAAAAAAGGCGATTAAACTTGCCACCACTCAAGAAATAATAATTCCTATTTCATTAAAGTTATTAAACCGAAAAATAGATCCATTTTTAAAAAAAATACCAATTCCCACAACGCTACTAATCATAATTGCAATAGATGTAAACAAACCTATTTGCTTTGTTTTTTTAACACTAATCTTCATGATACAACCTTATCATTCTTTTAAAAAAGAGGACGCATTTTAACGACCTCTTTTTTAAAAAATTTAAAATTTTATTTTTATATTATACAATAGGAAATTAAAAAAAATAATTTTCTAAAAAAATATTTTTAATACTGTTTTTAAAATATTTATTAGTTTAATTGAATAATTATTAACTTTAAAGTAAGAATTATTATTGATTGTTCTTCGTTTTTAAAAACTCATCTCAACCAAGAATTGATGCGTTTTGGCTATATAAATCTTCTTCACCTTTTTTAACATATTGGCGTGTTTCATCGCTAAATCTGCGGTCAGTAAAGGCCCAATTTTTAATTTGTTTACCTTCATCCATCATTTTTTTAAAAGCTGAATTTTTAATTAAGCCATGCACACCCATTAAAATTAAATTAACATTTAAATTTTTAATAGGTTCATAATTTGTTGCTGTTCAATTAATGTGGTTTTTTTTAATACTAATTTGTTCTATATCACTTAATTGGTTTTTTATTACATCATTTAAATAATAAGCTAAGTTAGGATCTTCATGGACACTAGTTGATAGTTCGCCTTTAACTAATAAATCTGGTTTTAAACCCAAAGCAATACAAATATCTAAAACTTGGGAATTAGTTGCTCAAATTTTTCAATCATCACGGTTTTGATCGCGATGATATTTAAATGGCGAATCAATGCCTGTTTTAATCGTTTGTATACCATTTTTCAATTCTAATTCATCATTTGTTAGTAATTTTTTTAAAGTTGGAATTTTAAATTTTTCTTGTGCTTTCAAATGTTATAATTCATCTTTTGGCATTCCAAAAGCTTTTGATAAAGTATATAACATACGATGCATTCCGATGATACCATAACTTGCGTGTCAAACAATACTCATTGGTAAAGGAATTAAATCATTTTTTTGATTTATTTTTAAAAATTCACGAGCTAAATAACCTTTATAATTTTGGTTAATCATTCAATTAGCAAATTCTTGTGTAATTTTTAAATTACGATTGCCATTCTTATCATAAATATTAGGGACAATAGCATCATCATAAATGTAAACAACTTTATCTGCTGTTTTTGCAAATGAATTTTGTAATTTAGATGCTAAAACGCTTTTTTCAACAGAATTACCTTTAGATGAAATTAATGATCCAAACGATTTTCAAGTATCTAAAAGATCGACTGCATCCATAGATTTTGGTGTAGGAAAAAATGCACCGAATCCTGGTAGTTCTTCAAAATCAGGTTTAGAATATAATAATGGGTATTCAGCAGGTAATAAAATACCTGGAGCATTAATTTGGCTATCATCTTTTTGAATAGGTGCATAATTAATTAAAAATAGTGCTGTTTTCTTTTGCTGGCGTCATTTTTTTCAAAATGGTGAATTAGCATACATTTTAATATCATCTTTAATGATCCTAATTACATTTTCAATTTGTGCACTAAAAGTTGACTTATATTCATTTTTATCAATCATTGATTTAAAATTTTTAGATTTTGTTAAATCAATATATTTATCTAAATCTTTTGCAAGTTTGCGTGCTTGTTGAAATGGACTATTTCATCAATGAACTCAATCGATAAAATTATCCTTACCTTCTTTTTTATTTAATATAGCCTCAATAATCTTATTTGCTTGTTCTTGTGAATTAAAATTTTTTGTTTTTTGATTATTTTGTTTTGAGCAAGCGGCAATACTTATCGTTGCTATTACTCCTGTAAACAATAAAGCTAATGATGATGTTATTATTTTATTCTTTCTTTTCATAATTTTTTATAGATTCCTTTTATAGACTCCTAATATTATTTAACGATAAAATTTGGAATTTACGATAATCTATATATCAAAAATATAAAAAATAATTAATTTACCATAAATTGTAAAAATATAAAAGTTTTTTTAAAAAAAGAAACATTAATGAGTGATTATTTTAAATAATATAAAAGATTTGATTTTTATTCTATATTATTTTGTTAACTTTTTAGTATAATAATATTTAGTTACATCTAAAAAATCCAATATTTTTAATTTTTAAAACTTATTTATACAGAAATAAGAACTTTTTTTAAGGAGAGAAAAAATGCAAAAGTCTTCAATGCTTAAGAAAGAAGTCGCTATTGCGCGTCGACAATGATATTTAGTTGATGCAACAGATTTAGTTTTAGGTCGTTTATCTGTTAAAGTAGCAGATATTTTAAGAGGAAAGAATAAAGTTGATTATACACCAAACGTTGATGCAGGTGATTATGTGGTAGTTATCAATTCAGATAAAGTTGTTTTAACTGGTCAAAAAGCATTACGTGAAAATTGATATAATCATTCACACTATATTGGTGGTTTAAGAACACGTAGTGGGGAAGAAATGATTAGCAAATATTCAGATGAACTTATTCGTCGTTCAGTTAAAGGAATGCTTCCAAAAAATAAATTAGCAAAACAAATTCTAAACAAATTATTCATTTATAAAAATGATAAACATCCCCATGAAGCACAACAACCAACTATTTTGGAATTAAAATTAAAATAGAAGAAGGAATTAAATTATTATGCAAAAAAGTAATATTGTTGAATACAAAGGTTTAGGTCGTCGTAAATCTTCAATTGCAAGAGTTAAATTAGTTCCAGGTTCTGGAAAGGTTCTTGTTAATGATCGTCAACCAGAAAATTATTTTCCAAATAAATTAGTTATCCAAGACATGATGCAACCATTGGTTTTAACAAAAACAGCTGAAACTTATGATGTTTATGTTAAAGTTATTGGTGGTGGATTTAATGGACAAGCTGGAGCAATTCGTTTAGGAATTACTCGTGCTTTAATTCAAACTCGCGAAGATTTAAAAACCGATTTACGTAAAGCTGGTTTAGTAACACGTGATTCACGTGTTAAAGAACGTAAGAAATTTGGGCTATATGGTGCACGTCGTGCTCCTCAATTTACAAAACGTTAATTTATTTTTAATTATTAAACATTTAAAAATCCATTTTATATGGATTTTTATTATATCTTATAAGGTTATATGTATACAAAACTAACATGAAATAATTATGAATTTGTTGAGCAAATAAAAGAAGATATTAATCCTGGACAACAAATTTCGACCTTGACATTATTATTATGAACTTATTATGGTTATGATATTTATTTTGAGTATTTAAAAGATAAAAATATTGTTCTATTTTATGCACAAGCAAATGAAGAATTAAAGATAATTGAACAAAATAAAATTGCAACACAGTTTATTGGCAAATTTTTTATTATATTCGCTTATTTTGATGCAAAAAAATATGATTGAACAACATTGATTCCAATTGCTTTAGAAGCATTAAAAACAAATTTTAAAAAAGAATTTTTATTAGCAATTAATGGAATACAAGATGAAAATTATTTAAATAATTTATCTCAATTTAATTTTGAATTAATTTATAGTTGATATTCAAATTTTATTTATGAAACGCAATCATTAAAATACTTTTGTGGAAAAATTCTACAAAAAAAGCGTAATAATCTTAATTTTTTTATTAAAAATTTTAAAAATGATTATGAAATTATTAAGTATAATCCTAAATTTCATTTACATGAGGTTTGCACTTTCTTAAAAGAATGAGATGTTAAAAATTTTATTAATTCAAAAAGAATGCTAGTTAATTCTAATTCTGATTTGTTATTAAACACACAAACTAATCCTTATTTTATGGGATCACTTTTAATAAAAAAATCAATAAATAAAATAGTTGGATTTACATTAGTTTATACTCGTCCAAATATTGCAGAAATTCTTATTGAGCAAACAGATCGTGAAATTAGAGGTATGTATCAATATTTATTATCACAAAATTTAATAATTAATAATATTAATAATTGATTGATAGATCGACAAGATGGGGCGTGGTCTGATAATATAAGTGCTTCAAAATTATCATATGCTCCTAAAATAATTACTAAGAGAGTTAATGTTTTAATCAAGGAATAAAAAATATGATAACTGATAAATTAATTAAAGAATTAAATGAAATTACAAGATTATATTATAAAAATGATCGTAATCAATTTAAAAAATCTTTAATAAATATATGAAGCAAAACAATGGGTTATACTCCGAATTTGCAAGACCCAAAAACTTATAATGAAAAACTACTTTATATGTATTTAAATACAGATCAATCGAAAATTTTAGAAATTGTCGATAAAATAAGTTTTAAAAAATGATTAATAAAACATCGATTATATCAATATCAAGTTCCAACTTTAGCAATTTTTAATCGTCAAAATCAAATTACTTTATTAGATCTTAATAAGTTCCCTAAACCTTATATTATTAAATTGAATAATAGCACTGAAAATGAAGATTTATATATTATTCGTGAAACTACTTCACAAGCAGAATTAACACAAATAATAACTCATTTTAATAACATCTTGCTTAATAAAAAAATTAATCATCCACGTAATTTTTATGAAACATGATGTTATTCACAAATTAAACCACAAGTACTTATTGAACCTTTATTAGGAAAACAAACACTCGTTGCTGATTATAAAATATTTTGTTTTGCACAAGAGCAATTTTGTTTATATATGAATAAGGATGAAATTGAAAAAAATAATTGAAATTGGGATTTTTTAAAAAGTAATATTTTTGATTTAAAAAATAATAAATCAATTTTAAATCATGAAATAATTAATTTTGACTTTAGTGAAATAAAAGATGTTTGCCAAAAAATTTATGAAGTTTTAAAAAATGATTTTAAACACTTTCGTATTGATTTTTATTATGTTGATCATCGCTTGTATATTGGTGAAATTACTTTTAACACATCAAACGCTTTTTTTACTTTTTGAGATGATCCTCAGTGAAAACAAAAAGACATTGAGTGAGGTAAATATTGAAAATAAAATTAAAAACCAGCAATTTAACCTTGCAGGTTTTTAATTATTTTTTTGTTGATGATCGACCTAATACTTTTGTTACATTTGAAGCACTACGTACTTTGACGTTTGAAACACTAATTCGTTTTGTATCATCAAATAAATCATCACTAAAATAAATTTTCTTTCGGCGTTTCGCTTTCATTTTTAAAGCTTCACGTTCTTGATTTTGAAGATCTTCAATTCGTTTAATTTCTTCTGGATCCTGAATAACATTATTTTTAATTAATTCTTGGACAATATCTACTTTAATTTGCTCAGAAAATTCATGCAATAATCGCTCGTTTTCATTTTGAAATTCTAACAATCTACGGCTATCACGGCGTTTACCAAATTCATTAATTACTGAAATTAAAGCTGAAGCAAATAATGAAGTCAGAAAACCATAATAGGTAACACCAATTACACCTAAAATAACGGCAAATACTTTACCAGCTGGAGAAACAGGTGTAACATCACCATAAGCTATCGTGGTAATTGTTCCCATAGAAAACCACAAAGTTTCACCAAAATTTTTAAGTTTGTACAACGGATTACCAGGCAAAATATGCTTTATTTCATAATAATTTTGTTCAATTCTATAAATAATAAAACTTAATAGAAAAGTAAATAAAACTAAAATTAAGAATGATGATAATAATACACGTCAACGCCGTTTAAAAATTTGTCAAAAGAAATTAACTTCACTACTCAAAATAACATTTTTGATAATTTTAGAATGCGCACCTAAGATTTTAATTAAAAACATACAAGGAACAACATTCATATAAGCATTATTATTGATGATTTGATAATAATAATCAAATTTTTGTGAATATGAAGTTGTTTCATTCATTTTTGCTACAAAATCTAATAATCCTGTATTATAAATGATAACAATTGCTACAAAAGATACTAGATCCAATAAATTAACAAAACGAAATGGTGCTATTAAAAAAGCCTTTAGTCTTGAAATTGAATTGTATGTGTAATCAGCGTATGAAATACGAATTAAATAATCAATAATAAAAAAAATATTAAAAGCATAATAAACTTCAAAATATTTAGCAATAAAGTCTATGTGCTCTTCATTAGCTTGTTTTAATTCAATTTGATTATAAATAATAATTGTAACGGAACAAAAAGCAATCATTAAGACGAATAATTGATAAAAAAATGAGAAAAAAGTTTCAGGCACTAACATGCGTTGATATGCTTTCATATCTGTTCATTCATAAAAAAAGTAACGAATTCGATTAAAATAAATAATTTTATTTTTTCTAATAAATTCTTTACGCTCATCATTTGTCATCGTTTTATTTTACTCCTATTATTTTAAATAAAATAATTAAATTTAAAAACTTATATATTAAGTATTAAATAATTGACTCATTAACCAACTCGTATTCATTATCTATTAATGAATATAACATTTTTACATTAAAAACATAAATTAATAAATTTTTAACACCATCATTTTGAGCACGAACAAAGACTTCATCTAATACATTTAAGATATCAACATTATCTTTAATTTCAATAATTTTAACAAACATGTCTTCATCATCATGAAGATTAGAATATAAAGCATCATATCAATGATTATTTAAATCAATTTCACGCTTTGTAAAACCTTTTTTACTTAAAGCTTCATTGGCTAAATTTAATTGATGATTTTTAGAAAAAATCATGTTTTTATTACTATTTCCTAATTCGTTAAAGATATTTTTATTTACTGTCTCATCAAAATAATCTAATAAGTTAAACAATAATGCTTGTTGTCCAAAATTACGCGCATTACGATTAATGATGTAATCCAATTTTAATGAATTATAAGTTATATTTTTCTTAGTGATTTCAGATGGACGACCATAGACTGTTAAATCATTCATTAGCGTCTCAAATCCTAATTCATCCAAAGCTTTAAAAACCACGAATGTAAAAAAGTCTACAAAAAATAATTGTGATTCATCGTTTAATGGAATTTCTGTTTTTGAAGCGTGTTTTTTAATGTCTAATAAAAGTGTTAATAACTCAGTATTTTTTTCGCTGTAATCTAAAAAACGATATAAAGAGTTGACAAAGAAAGGAAATTTTTGTAAAAGTTTTCGTGTATTAACATTAGCAATGATTGATAATTGATAGAAAATAAAACTCATTAAGCGACGTCCGCTTGTTTTTGTTTTATAACGATCCAAACGGACAACATTTGTTTTCTCTAAATCGATAATGTTACGGAAAATTTTACGTTCGTCTAAATATTTAAAACCACTAGCATCGAAGATTACTGTATTATTTAATTGGTTTAAGGTTTTGAAATATTTTAATATTTTAGGATATTGATCACTATTAATATCTACAACATCATAAAAAAATGTGTAATTATTTTGAACAACGAATTTAGAACGTTCTAAAAAGCGTGAATAAAAAGTTACTTTAGCATTAATCTTTTCTTGAGAATTACTTTCGATAAAATATTTATCATATATTCTTTTAAGACCTTCGGTCATGTTTGAAACAATATAATTACGTTTATTATTTTTTAAACTACTAAATAAAGCGACAAAAGCTGAAATAATAACAGCAACAACTAAAGAAAATAGTAAACTTAAGAAAGCAATATATCCATCTTTACCCCACAAATTAATAATTGCATCACCCCGTAAAAAAGTAACAGATATTAAAGGAACAGCTAGTGTAGTTCCTAAAGTACTAAATGCTGTTAAAACAGGCATCAAAAAGATTTTTGAACGAGATGTTATTTCACGGTCTGCGAACGCTGAGTTGATTTCTTCAATCTTTTTAACAACAGTATTTAAACGGCGTCTTTTCCACCAATTAATTAAAGGGTTATTAGAAGGCAAGTTTTTAAAGTTTTCTAAACTTGCATTTGACAGTAAAAAATTTTTAATATCATTAATACATTGGTCTTCAGTTGCGCTTGAAATTGATGATCAATTAGGAATTTCTACATCCATTAAACTAATTAGATCGTTCATTAGTTTATAAGAAGTGTTTTCGGGAACGCCATATAAAGCTACAACTCTTTTGTTTTTTGATACTAATGTTTTGGTCAATTTTTCGATTTCCAAATCAGATAAATTATTTCTCATATAAATTTAGTTTGCTTACTTAATCCATTTAATTTGTATGTTATATAATTATACAACTTTATAACAAAGTTATTTTTTAAATGTAGATTAAAAATATGAATTATATTAGCACAAATAAGATAAATAATTTGATATTAAAACTTTCTTATTTTTAGTTAAAATAATCATATTATAAAAAACGAGGTATCCACAAGTATGAAAAAAGCGATTAAACAAAAATTGATGAATCTTGTTAATTTAAATCCGCATGATTCAATTATTTTAACAAGACTTTCATCATCAAGAAATATAGACTTATTTAAGTATATTAAAAAAGAAGATCTAATTAGTTTTGTTAATGGCGAAATTAGTTATGTTGAATTAAATGTAGGTAAGAATTTAAATTTATTTGAAGAAAGTTTAAAAAATGCTTTAAATATTGGTGAATTTTTAGAAATCTTAGTAAATTACAATGTAAATTTAGCAGATAATAAAATTAATTTACTACAACGTGATTTTCATGCAAATAAGAAAAAAATTATTCCTTTAGCAATTGAACAATTGTTAGCATTTAGAAAAAAGTTTATTTCTATTAATAGACTAGCAAAAAATTATTTTGAAGATACAACTGTTTGACCTCTTTACTTTACTTTTAATTTTTTGAAAGGAAAATTATTACCAAACGATCCTTTTAAATCACCATTAACTTTATTTAAAGTTGAAATTCGAGAAGAAGCTGATAAAATTTTTATCGCTAAAATTCAAGATGAGCCAATTGTTAATGAAAAAATCCAAATCTTTTTAAAAAAAGGTTATCAAGATTTTAAGGTTGAAACAACAGAATTGTTGACTAATTATGAATTGTCTTCAACTATTAAAATGATTGAAGATATGACAGGTCAAAAAATTAATATTAACGAAAACGAATTTGTTCCTTTCATGAACGAAAATGAGACACAAATTTTAGATCGATATACAACTTTTGAAATTGAACCATCAGCTGTACTGGGGTTATTCGAACCCGATGGGGGCGCTTTAAAAGCTGATTTACATAAAATTATTGAAATGGATGTTGATCCATTTGAATCAGAAAACGATGGTTTGAATAAACCAGTTGAATATTATGAAGATAAAGTAATTAAAGAACAAATGGTGGTGGAAATTGGACGTCCTCTAAATATTTTTCAAAAATATGCTGTTGCATCATCACTTTCACAAAATACTTTGATTTATGGACCTCCTGGAACAGGAAAGTCTGAAGTAATTGCAAATATTATCTTTAATGTTTTATTAAAAGGAAAAAGTAGTTTATTAGTATCAGAAAAACGTGCGGCTTTAGATGTATTAACAGAAAGAATAGGTTCGCTTTCACAATTTGCTTTATATGTTTATGATTTAACAAACAAAGAAACTTTTTTTGAAAAAATTTCTAATTTAAATGATTTATTAGGTACACAATGGTATCGTGAACAATCTCGTTCTACAAAGATTAAAGAAATAGAACCAATTAAATTTACTCCAGAGGAATCAATGTTTTTTAAAAATTATGAGGATTGAAATTCTGAATTACTACAATTAGTTAAAAAACACTGGAGTATAGAAGATTATAATGATGGCATTTATAAAATGGACTATGCTGATTATGTGGCTACCAAAAATGAATTAGGAGAACAAATTGTTAAAGAATGATTAACTCCCCAACAATTTAATGAAGAAAATGAAAAACGAACAACATTATTTGAAGAGATCAGCACTATTTTTAATGAATACAATTTGTTAAAAATTGAAGATTTATTTACAGCTTATCTAAGATTTACTTCTTTTATTAAAAAATATAAGCTAACAGATAATTATAGTTCTATAGAAATTTTAAAACATTTAAAAATGATTACCAATAAAATTCAAACCAATGATGAATTAGTGACAAAATTTTTGATGCATGCTAATAGAATCACCAAAGACATTGATAATTATTACAACTTTTTAGCTGAACATAATCTTGAATCGAACACTATTTTTATGAATAAATCAATTCGTGATAAACGTATTTTTATAGACAAAATTGGTGATTATTTAAAGTTCCGTAAAGATGTGATAGATAAAGATTTTAGTTTGCAATCAAAAACAACACAAGAACTAAATGAAATTATTGATTTATGTGATAATTTTTTTACAAAACACAAAAAATTATTAGCCAAAAATGAATGATTTAGTTTTCTAATTAAAAACAAAGATCGTATTTCAGCATTTTTATCAATTTATAATAATGCAAGTGATGAAAACAAACAAATTATTTTTGCTGAATTTATTAGTAACGGTACAATTATTACTAACGCTGACCCAAGCGATGAATCATCATTAAGTTTAAAAGAAATTAAAACACGCAATCGTGATAGTCAAGAAGTAATTGAATTATTCATAGATTTTTTAAATAATATTGAATATTTATCTAAACCAAAAATGGACCAAATTGCTTCGTATCGTGAATTTATCAACCAGGATGTTGATTTTTTGTCAAAATTACATACGCTTGCTGAAATTTATACACCAACAATGCAAGATATTATTCGTGAATGATCATGATTATCTTTACCATATATTAAAACGCTGTATTTAGAACCATTAATTTTATTTGATCTTGAAAAGATTGGTAAAATTATGAAACATGTTTCAACATTAATCACGCACGATCAATTTAAGAAATTAAAAGTGATTGTTTTATGAGATGAAATTACACATATAATTCCAATGTTTTCTGAAACTAAAGGGCGTTTATTGCAAGATATTATTATTCAATTAAGACGTGAATCAATGCGCTCCGCTAAAATTGTTGGTGAAATTGTTTTTAAAAAATATATTAATAATTTAAGAAATTATTTAACAAAACTACCTCAACAAGAAAAAGATGAAATTACTAACGCATTACGAATTGCTTCATCACGTTCTTGACCATCAATTTCACGTTATTTATCTAAATATTACACTGCTTTAAAACGTTTATTTCCGATTTGGGTTGCGCGTCCAGATAATGTTGCATCTTTAATTCCATTAGTTGAGAATGAATTTGATTATGGAATTTTTGATGAAGCATCCCAAATGACAATTGAACGATCATATCCTATTGTGTATCGTTGTAAAACTAAAGTTGTCTCTGGTGATGATAAACAATTAAAGCCAACATCATTCTTTATTAATAAGTTAGCTGATAGTGACTTTGAAATTGATGATTTTGATAAAGTAGATTCTTTATTAGAACGTGCTAAAACTTCATGATGAAATGAATATCATTTAAAAAATCATTATCGTTCTGATTCAAAAGAGTTAATCGAGTTTTCAAATAAATACATTTATAACAATAATCTAGAAGTAGCCACACGCCAAGGTGCTTTTGAAAAAGGAATTGACGTTATTAATGTTAATGGAGTTTGAGAAAAAGGTAATCCATTAGAAGCAGAACAAACAATTGCAACATTAATTGATAATTGAAAAAAATATGAAAAAATCTTAATTGTAACATTTAATGCTATACAAGCTAGTCTAGTTGAAAACCTACTTTTTGAACGAATGAATATATTTGAAAAGGGATTGTGTGACAAAATTGAAAATAATGAAATTGTGATAACTAATCTTGAAAATGTTCAGGGTAACGAAGGTGATTTAGTTATTTTATCAATTGCTTATGGCCCAAATCCTGAAGGAAATTTACGTAATAATTTTGGACCACTTAATGCTAAGGGCGGAATGAATCGTTTAAATGTAGCGATCACTCGTGCACGTAAAAAAATGATTGTTATAAAATCACTTTATGGTCATCAAATTCAAGTTTCAAATCTTAATAATCAAAATGCACTGACATTTAAACGCTTTATTGAATATGTTGATCGAATTAATGGTCAATTAAGTATTAATGATTCACTTGAAAGTTTAGAACAACAAACATATTTAGAATTTGATAATGATTTAGTTAAAGAAATCTATAGCGAACTAACAAAAAAATTATCAAATAAATACCAAATCTTTCCAAATTGAAACATTGGTACTAAAAAAATTGATTTAGTAATTATTAAAAAAGAAACTAAAGAAATTGTAAAAACAATTCTACTAGAAACTTGAAAAGAAAACCGTAGTGTTCAGATTATGTTTGAGGATATTGATCGACAATATTTTTTAGAAGATCGAGGTTATTCAACTTACAGAATTAAAGAGTATGAATGATATATTGATAAACATAAAATTGTGTCACGAATTAATGATTCGTTATCATCAAATAATAACAATAATAAAATTGATTATGTTTTATGGCAACAAAATAATTTTTAATCAAAATATTCACTAATTGTACAAAGTATTAAGAGTAGTATAATTCTTAATACTTTTTAATTTATTAAATATAACTTCAATATCAACTAAAATAATAATCATTTTTCATATTTTTAACTTTTTCTAATATAATTATTATTAATAATATATATCAAGATTTGTATAAATGATGTTACAACATTGTTTATATGTTTATATAAATAAATTTTTAATAATAAAACCAATTATAAGGAGTTAAAATGGAAAATACCAAAAAAAGTCCTACATATGTTTATGCTTTAGGGGGGCTAGAAGAAATTGGTAAAAACACTTATGTTGTTGAACATGAAGATGAAATTATTTTAATTGATGCGGGCATTAAATTTGCTAATGCATCTTTACCAGGATTTGATGGAACTGTTGCCAATTTTGATTATTTAATAAAAAACAATCATAAAATTCATTCATTAGTAGTTACTCATGGTCATGAAGACCATATTGGGGGGATTCCTCACATTTTGCGACATGTTAAAATTAAGACTATCTATGCACCAACATTAGCAGCAAAATTAATTGAACGTCGTTTAAGTGAATATAAAGATATTAAACCTCCGCGTATTATTGTCTTTGAAGATGAGTCAATGTATAAAACAAAATATTTTGAAGTTGATTTTTATCGTGTTTGTCATTCAATTCCTGATTCATTTGGAATTTGTGTAAAAACACCAAACGGATACATTGTAACAACTGGTGATTTTAGATTTGATTTTGCTACAGCTGGTGATGAGACTAATTTAGCTAAGATTTCACAAATTTCTAATCGTGGAATCTCAGTATTAATGTGCGAATCAACAAGTGCTGAAATTCCTGGATTTAGTGAAAGTGAACGATATGTTATTGATAACATTCGTGATTATATGGTAAATATTAAAGGACGAACTTTTATTTCAACATTTGCTTCTAATTTAGGTCGTGTTGAAGAAATTATTGCTATAGCTGTTGGTTTAAATAAAAAAATCTGTATCATTGGTAAATCAATGGAAGCTAATATTAAAACAAGTCGAAAATTGGGTTATTTAAATGTTCCTGAATCTTCATTTATCACACATAAAGAATTGCCATTTTATAAAGATCATGAAATTGTTGTAATTTTAACTGGTTCACAAGGTGAAAAAATGGCGGCTTTAAATGTTATGGCTAATAATAACCACTCAAAAATTACTTTAAAACCATCAGATACAATTATCTTATCATCAAATCCAATCCCAGGAAACTATGCTCAAGTCGAAGCAATGGTTAATAAACTTTATAAACTCGGATTAACAGTTTATGAAAATTCACCTAACAAAAAGATTCATGCTTCTGGTCATGCCACACGGAGTGAACACCAATTAATGATTAAAGCAATTAATCCATCATATTTATTTCCAATCCATGGGGAATATAAAATGTTTCGTGCTTTAAAACAAAATGCTGTTGACCAAGGTTTTGATAAAGACCATGTCATTATTGCACCAAATGGCCAAAAATTACAATTATTAGATGGTGTTTTAAGTCATACTAATATTTATGTTGATGCTGAACCTAAATTTATTAATGGTTATGAAATTAGTTCTAAAATTAGTAAATTATTAAGTGAACGTGTTGTTTTATCCAGTGATGGTATTTTAAATCTAGTTTTAAATGCTGATTTTAAAAAAGCTAAGTTAAACTCTGCTGTAAGTATTTCAACACGAGGATGTTTTTTTGCCAAAGAATCAACTAATTTAATTAATAAAATTTCTAATGTAGCAAAATCAAGTTTAGAAGAAGCGTTGGCAAAAAAAGAATTTGATGAAAAAAAACTAAAAGAAATAGTTAGTGGAAATGTTAAATCAATTGTTTGAAAATGACGTAAGAAAAATCCAATTATTAATGTCACAATTATTAATAATGATTTGGTCGAACAGTTTAGAAAAGACAATAATTATGTTGAATTTATCAAACAAGCTGAAGTTGAAGAAATTGAACAAGAAGTTGATATTGATGATTTAATTTCAAACGGTTTATAAAAATTAGAGCCTTTAAGGAAATTATAAAATATGCAATATAAGATGTTAGTTATTGATCTAGATGGTACTTTATTAACGAAAACAAAAAACATTAGTAAAGCTAATTTACAAGGATTAAAAAAATATATTAGTTTAGGCGGTAAGGTTGTACTAAGTACAGGACGTTCATTAGAAAATACTTTAAAGATTGTGCATTTAATTCATGATCAAATCAAGGAATTAATCCAATATATTTCATGTTTTAATGGCTCATATATTTATGATGTTATTAATGAAAAAGTATTGTTTGAATCAACAATTAATAAAGATATAGTGAATGAAATCTATAAATTTTCATTAAAACATGGATTAGGATTTTGACCATATAACCAAAGGTTTATGCAAACACATTTTTTAGATGTTTATAATATTAACTATAAATTTTTATTACAACTTCATCACACAAAAAGAAAAGTTTGTTTAAACCCAATTTTTAATAAAAATGATAAATTTTATAAAATTAATATTTTACCTTCAAATTTTGTTAAAAAATTAAAACACGAGGTTATAGATCAATTGATTAAAGAATTTAGTGATCAAGTAAATATTTCATTCACCTCAAAATACATTGTTGAAGTGACTAATAAAAATATTAATAAGGCATCATCATTACGATTTATTGCTAATTTATACCAGATTAATTTAGATAAGGTTGCAACGATTGGCGATTCGCCTAATGATATTCCAATGTTTAAAATTAGTGGTTTAGCAGCGGCTGCTAGAACTAAATCTAAAACAATTATTGAACATGTTGATGTGATTATTAATTATAAAAATAATTCTAATTCTGTTGCATTATTTATTAATAATTACTTACTAAAATAAAAACCAAAGCGTTAAGTAAAAATACTTGACAGCTTGGTTTTTTTTATTTATAATTTAAAAGTTATTTTAATAATAAAACAAACGAGGTGAAAATTTTGGTAAAGATTAGATTAACAAGAGTAGGAACACATAAAAAACCATTTTTTCGTATTGTAGTTATGGATGCTAAAACCAAAGCTAATGGTGCATATATTGAAAATTTAGGTCATTATGATCCTGTTTTAGGACAAGTTGTTTTAAAAAAAGAAGCTATTTTAGCTCAATTACAAAACGGAGCACAACCTAGTGAAACGGTAAAAAATATCTTATCACAAGAAGGTATTTGAAAAGAATTTATTGCTTTAAAAGATGCAAATAAAAAACGTAAAGCTGCTTTAAGTAAAGCTAAATAATTTATAACATTAATGAAAATATCGATTTTATCTTTATTTCCTGAATTATATGAAACATGGATAAAGCATTCAATTATTAGTAATGCTATTAAAAATAACCAAGTTATAATAGAAGTTATCGATTTTCGTTTATATACAAATGATAAACATAAAAAAGTTGATGATTACCAATATGGTGGCGGAGCAGGTATGGTTTTAAGGATTGAACCAATTGTTGCTGCCATTCGTGCAATTCGTTCTTCTAATTCATATGTGATTTTAACAACACCAAAAGGTCAAGTTTTTAATCAAGAATTAGCAAACAATTTTGTTAGTAAATATGATCATATCATCATTATTGCTGGTCATTATGAAGGTTTTGATGAACGTATTAATTATTACATTGACGCCCAATATAGTATCGGTGATTTTGTCTTAACAGGGGGGGAATTACCAAGTATGGTAATTTCTGATGCTGTTATTCGTTTGTTAGATGGCGTTATTTCTCCTTCTAGTTTAGAAACTGAATCATTTAATAACTACTTATTAGATTATCCTGTTTACACACGCCCTCTTGTTTTTGAAGGGCATAAAGTACCGGATATACTATTATCTGGTCATCATAAAAATATTGCTGATTTTCGTAAACAGCAACAAGAGACGATTACTAAAAAGAATCGTCCTGATTTATATCAAAAATATTTAAATAACAAAAAGTAGGTGTAAAAAATGGCTTTATTTAAGATTAACAAAGGCGAAATTATGAATTTTGTTAATTCAACACAATTAAAAACAGATATTCCTAATTTTGATTCTGGTGATACAATTATCGTTCATAATCGAATTGTAGAAGGAAAAAAATCACGAATTCAAAAGTTTGAAGGCGTTGTTTTACGTCGTCGTGGTTCTGGTTCAAGTGAAACAGTTATTGTTCGTAAAGAATCAAGTGGTATAGGTGTTGAACAATCATTCAATATTCATTCTCCACTTGTTGAAAAAATTGAAGTTATCAAGTATGGTAAAGTAAGACGTGCTTATATTTCATACATGCGTAATCGTTCAGGCAAATCTGCAAGAATTAAAGAATTAAATAAACAATAAAATGTTTTATTTGAATAAAAACAACAATTACTATAAATAGTTGTTGTTTTTTTTATGTTTATCCTTAAAATATTATTTTGAGTAGAATATAAAATTCTTTCTTTTTATAAAAAGACTAAAAGGGGTTGATTTAATTGAGTAATAACGAAGAAAAAACGCATCATAAAAACAAAATTAAAAAGATTTTTAATTGAACAAATAAAACTAAAGAAGTATTAAGAACAGAAAATAATTTATTAAAACAAATAAGCAACGAAGCAACTATTCAACAAAGTGTACAAATTGAAAAAATTCGTTATAAAGCAGGCCTTTTAAAGTTTGCCGCTCTTTATTCAACTAAAAAAATCTTCTTACGTTACTTAATTATTGTTATTTTTTCACTTCTTGCGAGTTTATTAGTTTTATTATTAGTTCATAATACTGGAATTTATTCAGCTGGCATTATGGGAACATCACAAGGGATCGCACGAATTTTACAATCAATTATGATTTCAAATCATAGTTCACCAAAAGAAGCCAAATTAGCATACGATATTATGTTTTGATTATTTGCAATTTTTGTAAATATTCCTTTATTAATTTTTTCTTATAAAAAAATTGGCAAGCATTTTACATTAATGACATTGACATATATTATTACTGTGCAAGTTTTTGGTTTTGCTTTAAGTCAAATTCCAAACGTAGAAAAAATTATGATTTTTGGCGACAACCGTTTAAGTTATCCACCTATTAATTTGTTTTTTACTAATGGTATTTTAGATGAAAAAATAGTTAAAAATATTGAAACTGGTGTCTTGAATTATTCTAATATTACTAATTCAAAAATTGCTGATGAATTTATGCATTATGTACAAGCAAATCCTAATGCGACAATTGGTGATTTATTTCATTTAAACCAAAGTTTGACTTATCGACAATCTTTGTATGAATTTGCTAAGCCCTTATATATTTTTCATGATCTTATTATTAATAAAGTACAAATTTTATCTTGAGTAGATCCTGATCAAGCAAGTAAAATTCCTTCAATTCTAATTTATGCAGTTATTTATCCAATTTTTGATGGTATCTTCTTATCGATTGTTTATATTGCTGGAGGTTCGTCAGGTGGAACAGATATTATCTCATTTTGATATTCTAAAAAATATGGAAAACCTACCGGTTCAATATTAACCTATTTTAATGTTGCAACATTGATTATTGGAATTGTTTTAGGATCATTCATTCCTGCTGGTATGATTAACTCTCGTTATTGAGATGCACAATATTTCTTTTCACCAAATATGGTAGCATCAATTCTTGCTTCAATTGTTTTAGGAATTGTTTTTAACATTTATTTTCCAAAACATAAAAGTTTGAAAATTCAAGTTTATTCAAAAAACGTTAATGCTATCATTGAAAATTTACGTGCTAATGATTTTAATAATTCAATTACTTTAAATAGTTTAAGTGATTCATTAACATTAAGAACAAATTATTCATTAGAAATTATTTCTCCATATATTGAGTTACCATCTTTAATTCATTTAGTGCGCGATATTGATAAGAATTGTTTAATAGTGATCTATCCAATTATTGATATTGATGGAGAAATGGTTGTTAAGAAATCTACCATTAGTTAAAAATTTAATGAGAAATAAAATGGTCGTCGTGACCATTTTTTTTATTTTTTTTGAAATAAATTAGTTAAAAATAAGTTTATTAATGATTATTTTTGTAAAAAAAAATGTAATATTTGGTATTTAATTATATATTTTAGTAAAAAAACCTTATAATATAACACAATAACCAAAAGGAGAAAAAAATACGATGATTAATTTAAATAATAATCAAAATAAATTTTTTAATCATACCATTCGCATTTTTTATGGCAAACGTATTTTTTGCTCTCGTTTTATTATTATTTGCATTTCTATTATTTATAAGATGTATATTAATACAATTAAAATTAATAAGCGTTGAAGCATTATTAACAAAATGTATTTTTAATAAATCAAAATAAATTAAAGGAAGTGTAGGCTCTTTAACATAAGATTTTAGGGCAAGTATATATGAAAACAAAAAGTAATCTCAAAGCAAGATTAATAATCTTTATTAGTATATTATTAACAACTATTATTAGTTTAGTTTTATTAACTAGTTGTTCAACAAAAAAATCTAAAAAAGTAACAATTAATCAAGATACGCAAATCAAGTTTCAATCAATCACAAATAATAATATTAATCTTGCAAATGAAAAACAAATATTAAATCAATCTCAAAATAAAGAAACTAAAATTTTTCTAAACCTCTATGAGAGTTTAAAAGATTATTTTGATCAAACAATTAAACGCTCCTTGTATTTATATTATTCTTATAATTTTGCTTTACTAAATTTATATAATGCAAAATATCATGTTAAGTTAAATGATTTTAGTTTAATTTACAATACAGATAAAAATTCAAATAATGGTACTTTTGATATTAATTATAAATTAGGCTTAGTTATTGATGACATTGGTGATATTAATATACAAACTAAACCTCAATTTTATGAGCCAATTCCACCTGACAAAAAAGTGAGAAATGATGATTATCACTTTACTAAGAAAATTAAACGTTTTTGATTTGGTTCAAAAAAAGCAATAATTGATTATTTTAATGAAGCTATTAGCATGCAAATTAATTATTCAAATGTCAAAATTTATCATTCGATTATTAAATATCCAGATAGCAAAGAACATTATGAAGGATATACAATAACTGGTGGTAGTTTATATCGTTGAGTTAAACAAGCTATACCAACCGATGATAATCAAAACAAAGTCCGTTATATGATTAGTAAAATTAATGATTTAAATATTGATAACGATGTTAAATTATTTAATTTAAATGATCTTGTTTTAAAACAAATTTTTATTGTTGATCGTCCTGCACAAACATCTATTAAACCCGAACGTGATCTTGTTTATACCAATATTTGAAATAATAAATTAGATGATTTTTACTTTTCACCAATTGCATTTTTAGCACCAACATTCATTAAAAAGAATGGTAAAAAAATTACACCTTTTAATTTAAAAACAAATAAAGAGTATTTAGATAATTTATATGAAAATCTTCTAATTCAGGGGCAAAAAAACAATAATGCTCTAATTAAAGGAATGTATGTTATTTATAGTATGTATAGTTATTTTGATTTAACACTTGAACCTAAAAAACCAGGTAGTATTCATCATTGCCATGCTGATGGTTTTTGTCATTAAAGTTATTAAGAAAAGGAGTAATTATTAAATAATAATGAGTATAAAAAAACAATCGTTTCCGAATCGTTTATTAAATTATAAAAGCATTATTGTTTTAATTGCATTATTAATATTGATGGTTATTAGTCACTTAATTGTTGAATTAATTAAAGATTATCACCAATACCGTATGAATGTTTTTATAGCTTATACATGTATCTTTATGGTAGTTTTCTTATATATTATCTTCTCGCGCCAAGTTTATCGTCGCCGTTGAGCAGTTAAATTAAGAAAATATAATGAGTATAAACTTAGTTCGTGAGAAATGAAATTAGTTGATCGTTTTTATATCAATGCTAAATATGAATTACGATCTATTGATTATGCCACAATTCGCTTAAATATTTTAATTACAATTATCATTTTACCTAGTTTTATTTATGCATTAGCTTGTATGATTGCTTATATTATTAAAACCACACAATCAATTACTTAAAATAGGAGATTTATATGAATCAGAAAAACAGCTTTGAAGCTAATTCATCGCAAAACAATTATGGTGAATTGCCAATATTAAACCAAAAAAAACGAGTTAATTATTCATTTAGTAATAATTTTATTATTTTTGGTGGTGGTCGTTCACTAAAAACAGCAGCAGATCGCGATCGTGTTGTTGATCCATTAATCGATTTTTTCACTTTTGATTCAAAGTTTATGACATTTATTATTAAATCTTTGAAATTAATTGCTGAATTTTTCTTATTAGCTTGAATTGTAATTACAATTACATTCTTTTTAATTAACTCAGTACCTGGAGAATCTGCTTTAACGATCGGGATTACATCACCAGAAGCGAAAAAAGCCATTCTAGCACAGTATGGTTTAGATCGTCCAATAGGCGTTCGTTATGTTAATTATTTAGCAAATATTTTTAGAGGAGAATTTGGAGTAAGTACTTCAATTCGCCCAGGGGTTGAAATTAATTCATTTATTTGATCTCGTTATTTAGTAAGTTTTGCTGTTGGAATTTTTTCAGTATTATTAACTCTAGTTATTGGAATCCCTTTAGGTATTTTTGTTGGGCGTAAACCAGGAGGTATATTAGATAGTGTATCAACCGTTATTATTTCTGTAATTTCAGCTGTACCTTCGCTTGTATTTGGGTTAATCTTGTTATTAATTGGTAAAGAAGTTGGATTACCATTTACGTTTGATATTAATAATTTTGTGACTTATATTTTACCAGGGTTAGCATTATCACTTGGTTCAATTATTGTTTATGTGCAATATATTAAAGTTGAAATGAATCGTGAATTAAACTCAATGCATGCGAAATTTGCGTATTTAAAAGGTGCAACTGTTAACCGTTTTGTGTGATTACATGCTTTAAAACCATCATTATTCCCAATTGCAACATTCTTTCCATTTGTAATTTTAGGATCATTTGTTGGAGCGTTGTTTATTGAACAAATTTTTCAAATTCCAGGTTCAGGTTCATTATTTTTTGAAGCAATTATCTCAAAAGATTACAACGTGATTTTATTATTAGTAATTATTTATTCATTAATTACAATTATTGGTTATACAATTCGAGATGCTTTATATCAAATTATTGATCCACGTATAAGAAGAGGAGGAAAATAAATTTATGGCGAAGTTTGGAGAATATTTAAAACAAAAATCAAAAAGTATTACTTCATCATTTATAGATGAATTAACATCTGATGCACCAAATAAGTTTTTACAACCTTTTCAACACCAACAATGAAAGATTATCGGTCATTTAATTGAGTTTAGAGATAATGCATACATGGGAGGGCGTCCTCGTCCTTTTCGTGAATTTGCTAACCGATACGGACGTTCTTTTTCTGGATTAATCGGATTAGCAATTATTATTATTTTATTTATTTTGGCAATTATTATTCCTTTTACAACAGGATCACCAACAGAATTACGACCAAATAAACGATATTTAGATTATTTTACAGATGGTTTTATTTTTGGAACTGACAATAATGGGCGTGATTTATGAGCCATGCTATGGTGAGGATTACGTTATTCATTAGGAATTGCTATTATTGTAACTGCTATTGAATTAGTAGTTGGAGTAACTTTAGGGATTTTAATGGGTTATTTTGAAATGTTTGATAAAATCATGACATTCATTATTAAAATTTTAACAAACGTTCCAACAATCATCATTTTAATGATTTTAACAATTATTTTAAAACCATCATTTGGAGTTATGATTTTTGCTATTTCATTTTCTGGTTGAATTGCAATGGCTAATCAAATGCGTTCACAAGTTAAACGTGCTCGTAGTTTTTTATGGGTTGCAGCTTCAAAAACATTAGGAACAAAAGCATGAAAAATTATTTTAAATTTCTTACCAATTTTAATTCCAATGATGATTACTCAATTAGTATTTAGTATTCCAGGAGCTATTTTAGCTGAATCATCACTTGCTTTTATTGGTTTATCTTTACCAAATACACCAACTTTAGGTAATTTAATTGCAGATGGTTCATCAATCATTACTTTATATCCACGTTTTACTTTAATTCCATCATTTTTACTAGTTTGCTTAGTTGCTTCAATTCAATTAGTTGGGGGTGCCACACAAGACGCATTAAGACGTCAAAGATAATTTATGAAACAAAAAAACAACCGATATACTTATATTAACTATCTTCGTAGTCAACGCGCTTTTATTCAAACATTAAAACCACAAATTTTAAAATTAACTTCAAATAATGTTTGTTTAAATGAACAAGAGGTGGATTTAGAACCACAATTAAATAATTTAGAAGACATTAATATACAATTAACTAATGAAAAAGTTAATATTCAAGATATTGTTACTTTAGAAGAAGAAATTAATGTTAAAGATAATAATCAAAATCAAAATTTAAATGAAGAAATCTTAGCAACAACATTAATTAATGAACAGGCTAGTGGTATTAATGAAATTAGTGAACAAAAAGAAACTACAATAGCTAATAAAAAACATAAAAAACAAACAATGGCTTTTAAAGCTGCTAATTCACGAAAAAACTTTGATTTTGAAAAATTTGAAAAAAATGTTAAATATAAAAGTTTTAAAGATGGTACAGTTAAAAAAATTGCAGCTGAAATCGAAAATGTACGTTTAACTTTTACAAACCCATCAAAACCCAATGATCGTGCTTTAGTTTTACGAAATACATCTGTTCAATTTTATGAAGGTGAAGTCCATGCAATTATTGGTGAATCAGGATCTGGTAAATCAGTAATTACTTCTCTTTTGTATGGTTTGGCTGGAAAAAATGCCAATGTTGAAGAAGGTAAAATTTTATTATATAACAATGAAGTTCAAGATTTTAGCTTCAAAGATTGAGAGAAATCACGTTATTTAGGGAAAGTAATTTCTGCAGTTTTTCAAAATCCAATGTCAACATTAAATCCAACCATGAAGATTGGTAAGCAAATTATGGAAGGGATGTTAATTAATGGAATTGTTAAGACAAGAAAAGAAGCCTATAATAAATCAATTGAATATTTAAAATTAACAAAAATTAATAATCCTGAAGAAATTATGGAATTATACCCACACGAATTATCTGGTGGAATGATTCAAAGAGTTGTAATTGCCTCAATTGTTTCATTACATCCAAAAATTTTAGTTTTAGACGAACCAACAACAGCGCTTGATCCAACAGTGCAAGCTTTAGTGTTAGATGTTATTCGTGAGTTACAAGAGAAATTTAAAATGTGTATTATTTTTATTACTCACGATCTTGGAGTGGTTGCTTCAATTGCTAATTATATTTCTATTATGTATGCTGGACAAATTATTGAAGAAGGTCAGCGTGATGAAATTTTATGATACCCACAACATCCATATACTTGAGGTTTAATTATGTCAATGCCTGACGTTAATAAAGGTGATCGTTTAGCAACAATTAAAGGTTCTGTTCCATCACGTTTAAATGACATAGTTGGTGATGCATTTGCTATTCGTAATGATTATGCATTAACTCGTGATTTTGAACATGAACCTGAAATGTATCATGTAAGTGAAACTCATCGTGTTAAATCAGCATTATTAGATGAACGTGCTGAAAAGTATACACCTCCACAAATAATTTTAGATAAATGAAATAATTTTAAAAAACGACAAGAAAATAATTAATAAAATAAGGAGCATCAATTATGAGTGATACAAACCAGAAAAAAGCCTTTTTTAATCACAAAAAACGTTTTTGTTTACCAGACAAAATTACTATTCGCCCAGTTACTGAAGGTTTAATAGAACGATTAAATGCAAATCCTGAAAAAAAGCGTTTAGTACAAGTACGTCACTTAGATGTTACTTATGGTCATGGATTTAAAAAATTTAGAGCGGTTAAAGATATTTCATTTAATATTTATCAAGGTGAAGTTTTAGGATTAGTTGGTGAATCAGGATCTGGTAAATCAACAACCGGATCTGCGTTAATTGGTTTAGCGCGTCATAGTTTTGGGGATATTATTATTGATGGTATTAAATTGCCTAAACAAGGTGAGAAAGTTACTAAAGAACTAACTGATTTTATGGTTAATAATGTGCAGATGATTTTTCAAGATCCATCGAATTCACTTAATCCTTATGTAAATATTGAAACAGTTGTTTCAGAAGGATTAAATAATATTAAAGATTTGAAAAAATCTTTCTTACGTTTAATTTATAAAGATTATTTACGAGGATTAAAAAAAGAACTTAAAGCAAAAAATTTTTTAAATAATGAACAAGTACAAATTTTTTTAGAAAAAGCACAAGATTTAAAGTTTGATTATTTAAATGAAACTGCTCAACAAGAATTAATCCAATTATTCAATGAATTAGAATTAAATCATTTATCATTAGATAATAATGATGAAAAATTACAAAAAGCTTTTAATGATATTAATACTTGAAAATCAAAAACAAGAACAGACATCACGCGCCATTTAATTTTAGATGTTTTAAAATCAGTTGGTTTAGATGAAACCGTTCTTCGCCGTTATCCATTAGAATTTTCAGGTGGTCAACAACAACGAATTGGGATTTCAAGAGCTGTAGCACTTCGTCCGAAGTTATTAATTGCTGATGAACCAATTAGTGCTTTAGACGTTTCAATTCAAGCTCAAGTTGTGAATATTTTTAAAGAATTAAAAACTAAATATGATTTAACAATTTTATTTATTGCTCATGATCTACGAATGGTTGAATATATTTCAGATCGAATTGCAGTTATGAATAAGGGTGTGTTGTTAGAAATTGGTCCAACAAAAGAAATTGTTAATAATTTCTTACATCCATACACACGTTCTCTAATGGAAGCTGTACCTTCAATTGATTCAGAAAAAAAATCACTATTAGGTTATGTATATGATGCTAACATGCATGAATATACACCAACAAAACAACCAAAATGAATTCATTTAGGAAACGATCATTATATTCTTGGGACAGATGAAGAAATTGCTAATTGAAAAGCAGGAAAATATTAGAAAAGGAGCGAAAGCAATATGAAAAAAAGGTTAAAATTATCAGCTAAAATTGCAATTGCTTTTGCATCAGTTGGTGCATTTGCTGGTGCTGTTGTTGGTTTTATGAAATTATATGCTGTTTCTACTGGTGGACTTGGACGTCAAACATTACAAGTTAACAGTAGTTTTGATACGACAATTCCTAATCGTCAAGTAGATCGTGCTTTATTAATGGATCAATTTGGCAAACCAGTTGCTGATTATGATTTTAAAGCAAAAAAAGGTAATAATACACAAGTAACATTATTAATTGATTTTGGAAAATATAAAAAAGGTCAGAAAATTTCTTATCTTGAATTTTTAGATAATTTTGTTAATTTAAATAACAACAACTTACCAAATTTAAGATTAGAAGTGGGCCCAATTGTTTTCAATAACAATTATATCAATAGTATTTCTCCAGACGAATTCATTGAGTTTACTAATTGATTCTTTAAAAATGTTTCTTGAGGACCGGATTTGTTGACTTTGAAAGAATTTAAATTAAGTCGTGGAATTCAACAAAATGGTAACGCAATTACACTAGGGTTACATAGTGGTACAAGAGAAAGAACAGTAATTGAATTCTTCCCAGATGCTTTTTTTGGTTCATTACCAATTTATAATATTAATGCTGGCCCCGGCAATGCTTATGATTCATTAGCACGACAAGTTAATAAAGATGCTATGACATTAGAAGAATTAGAAGAATATCATCAAAAAATTCCTTTAATGATTTCAGCATATAATAGCCATAGTTTTGGTGGTGTTAAATTAATTGATGTCCTAAAAAACGCAAAAAATACTTATTTATTTAATGCTGATAAGTACTTTGATTTAACAAAAGATCCACTAAATTTTCCTAATTTAAAAAATCTTAAAGCAAGTGATCGAACATTAAATTATCTTTTTTATGCTCAAAACGAACAACAAGCAAAACAAAAAGTACAAGCATATTTAAACGAATTACAATTTGTTAAAGGGTTAATTCCAAAAGATAAAAAATTTAATCCTAAAATCGACGTTAAAATGGATGAAATTAATAAAATTAAAATTAATGAATTTTTGGAATTTAATGCTAGTGCTTCATCTCCTTTTGGTGGTTCGCTTGATCTTGAATCAATTGGTAAATCAAGTGGTAATCAAAATTATTTACTAATTGATGCAACCATTGGTGGCCAACAACAAAAAATTAAATACTACATGTTTAAGGATGAATTCACTACTAAATCAAGTTCAAATGATGAATTTATTCAAAATGATAATAGTATTTTAGCATCAAGTTTACGTGAAATAATTACTAAAAATTTTAATGATTATATTGATCAAAAACAAAATTTTAAAAATGTTTATGATGTTGAATCTACATTTAAAAATAAACAATTTTATATTTATGATATTGATGGTAATGGTAAAAATCCCCATTTTTATAAATCAAAAGAATCATTATTAGAATCTGAATTAGTTAACTATGATGAGAAAAAAGTTTCATTGGTAATTATTACTAAAACAAAAGTCGAAGGTTTAAATAAACTAATTTTAACAACAAAAGATGATAAAGATTTAATTTTAGAATCACCTAAAAATAATCCAAAAGGTTATAATTATGATTCAAGTTTTGATGATGCTTTCAACACTCTTAAAGTTGCGGCTAATTATTTTGATGTTTTTACACCTCGTTTGATTAAACAAGGAAGTAAATTTGTTAACGGCAAAATTATTAAAACTTATACTATTTATGTTGATGCATATAGCGGTTTATTAGATAAGGTTTTAAATAAAAATCGTCATTTATTACAAAAAATTAATGGTATTCATACAGAAGTTGTTCAAGATAAATATGGTAAAAATACATATAAAGTTGTTAATGGTGAATACGAAGGTATTTATGTAACTGATCGAATTCCTTATTTATCATTAGTTGCTCAATCTGATCCTGCATTTAAAACAACAGGTATTAACTATTTAAAATATGTAAGTACACACGAATATGGACATCACCAAACATTACAAGACATGAAAGATATTAGTGATAGTAATGATTCAGTTATTGGTGGAGGAATTGATTCTAGATCGGGAGTTAGTGATGAATCATATGTAAATGGTAAGGCATTACAAGATTATTTAAATGCACGTTCTAGCGGAATTACCTTTAGAAAAACTGATGTTGATTATAATCCAACAAAAGATGGGTCATTCTTTAATTTTTCATTAAATAACGATCCTAAAAATCCAGTTTGAGAAACACAAAAAGATATTTTTGGTTCCGTTAATGCTGATGATCCTAAAGCCTTTTTCTATAATAAAAAACGTCGTTTTTTACAAAAATACGATGAATTATTTGAGGCGGCAAAATTACGTAATGTTAAACCATATGATTTATTTATTATGAATTCATTTGATCATGAATCTGCAACTGTTAACCCATCATTTGGCCCTGACATTAAAGATCCAAGTCGTTTAAAAGCTGAATATTATTTTTATAATGATCAAAATAGTCAAGAACAAAAAAATGATAATTTTAAATTTGGTAGTGTTATTGAAAAACCAGGACTTTCAAAATATGATGGTATTTTAAAAGATGGTATGGGAACACCAATTAAATTTAGTAAAGATGGTAGAGCAATTGTTTATAAACTTCACAAGAAAAAACATCCATACAAAAAAGATGATATTGAAATTTTAATTAAAACCAAAAACAATACACCAGTAATTGATTTATCAACTTGTTTAAAATCAGATGGGACAATTAACACAAGAAAACTTAATGAAAAAGTGAGAGAGATACAAGATTCAATTAATAGCCTAATCGTTAAAAATTACTATAATGGTGGTTGAGATGAAAACGGTAATTTTGATACATCAATGTTTAATTTAAAAACATATTTTGATCACCCAATGTTTACTTCAAATGAAAAACGAAAATGAGCTGAACGAATTACTGATGCGATGTTTAAATACCCTGATTTTAACATTAGCAAATCTTTAAAATTAGATGAAAAAGCGAATGCATCATCTGTTCCATATTATAAAAAAGTTCTTTCGCAAATTATTGGCGAAGATATTACAAATAAAACCTTTGACCAATTTAAATATGCTTTATTAACTTTACAATACAATGCATCAAAAATTTGAGATAATAAATCTAAAGAAGAAGTCAGCAAACTTGATCCAGAATTAATAGAAATTAAAAAATATTATGACAAACAGTTTGATCGTTTCGGTGTTAAAAATGTTAATGTAGTTGCTCAAACAACATTATTTAATTATTTTGACGCTGGAATTGAAGGTAATAATGGTTATAAATATTTTGTTAAACCAAAAGAAAAAGAACTATATCAAAATATTTTAAGAACAAAAACAAAAGAATCAGTTGAATCAATTATTGGTATCCGTCCTAGCTATCTTACACAAAATAAAATTACTTCATATGAACAATTATTTAATAAAAGCATGATAAATTTAAATGAATTTGGAACTATTAAGTTGATTGTTGATAGAGGTGATGGAAAAGATAATAAACATTTAAAAAAGCTTGACGAATTAACAGAAAAAGAAATTTACCCATTAATGATTTTAACGCCTGCTGAAGCACAAGCAATTAATTTTGATTATCTAAAATCAAATAATGGATCATTCACTCCTCTTACACTTGAAAATAATGGATCAAAATTCTACACAATTAAATTTAAAGATATTCCTTCTTTAATTGAATTTATGTCTGTGGATCCAGCTAAATATACAATTGTGGAAAATGCTTTAAGCTCAAGTCATGAAAATGTTCGTAAATGAGATTATGAATATTTAAAAGAACGTTATGATGTTGATAAATTTTTTAATGAAGTAATAAAAAAACAAGAAGCATATAAAAATCTAACACTAGAAGAGTTTAAACAAAATCTGACAGGTTTATTATTTGATGGATTTACAGAATCTAATGATATTTTTAAATTTTATAAATCAAAAGATTTTAACCCATCTGAATTAGATAAATACAAACAAGTGTTTGATGGCAAACTAGGTTTATATGGATTTAGAAGTTTTGGTAATAAATATGAAAGACCAGGCTCACCATATGGAGAGCCACTTGATACATATAATTTTGCTGGTAATCCACAGAATGTTTGAAAAGCAAAACCAAACCAAAAAAATGTACGTACTGTTGATTCAATTATTAAAGGAATTCAACTTGAAATTGAACGAAAACACCGTGAGAATAACACTTTAAACTTTGGTCAATTATTACAATATGCTTTTGGATTTACAATTTATACTGATATACCTGGTGGTTCAAAAGATATTTATGGTTGATTAGGATCATTTTTTGGAATTTCTCAATCGTCTGAAATTCCAAATGCTGATGGAACTGTTGTTTCATCAGATTATGTAACATTAAATAAAAAACGTGTTCAAGAAAAATTAAACGAAATTTTTGGGGATTATGTATTTAATATTGCTGAGGTATTAACACGTGATTATGTACAAACTGTATTTATTCCTTCACAAAATGAGTTAGATAACCTACCAAATTATATTTCAGGTTTAAGTGATTTTAATACAGGAAATGAATATGTCTTTAGTGGTGATAATACAAAACAATGAAACGAGCGTTTAATTCCAGTAAATAATTTCTTAAGTGTATCAAATACAATCGCATTAAATACCTTATTTGCAACAAATAATTACGAAAAAATTCAAAATGTTTTAGCAAATCAGGCTTTAAATGTTTATCAAAAAAATCAACAGTTATTTGATGATTATCTAACAAAACCACAAGAATTAGAAAGTAATTTTAATAAAGTTAAGGAAAGCTTTTTATCTAATGCTAACTTTTTAGCATTGACAAAAACTCATGATAGTGATGTTTTAAATAATTTGAATTCATATTCTTTTCTAACACGTTTAAGTCCTAAATCTAATTCATATATCGGTAAAACAAGATTAACAAATAATGGTTTTTTCAAAGACCGTTGATTAAGAAAAATTATTGATTGAGAGATTTATGATGACAATCGTGAACCAATTAAAGATGATCATTTAAATATTTTGGAATTAGATAATAAAACAAAAGTAACAGATCGTGCTCGAGCAATGTGACTATATATATTAAGATCAAAAGGTATTGGTGATCGAACATTAGCTCAAATTTATCGAAATAAAGAAAAAGATTCTATTTTAATGTATGGTTTCTTTAAAAAAGAATATAAGGATAAAGTTAAAAAAATTGCAATAAAAAATAAAAATAATGGCAAAATAGAATATATCGATGTTCACACAGATAATACAAATAATTTATTTTATCTAAAACGTCAATCAGACATTAATTCTAAATGAACATTAGAAGATGAAGGTTATGTTTCATGAACAAGTGATTATGCAATTTTATCTAATTTCACTAACCAATTAATTGGTTATGATTCAATTAATGCCAAAGGAAGTGAATTTGAAATATATTTTGTGGATAAAGATCTAAAAGAAGTTATGGATTTAAGTGATCCAAAAAACCCAAAAAGTTTAATGACTTTAGGTTCACGCAAATATGTTGCTGAAAACGGTAAATCATATACTGTTTCACCAGTTTATGCAAGAAACGAAAATACAAAAGATAAAAATCGTACAATTATTAGAATTTCAAACCAATTCTCAGTTTAGTAAATTAAGGAGTTTAAAATGAAAAAAATAAATAAGAAAATTATATTTTTATCTATTGGAATTGTTGGTTTAATTCCCGTTGCTGCGATTATTGCTTCTTGTGCTAGAACTAAGACCACTAAAGAACAAAATAAAATTAGTCCATATTTAGAAGATGGTAGTAATTTTGGTTTGGCAATGGATGCAATCAACACATTGAATTATGTAAAATTTGCATCGCTTCGTAAAATTGTATTTTCACTAGTAGATGGTTTAATAAAAGAAGCGCCATCAACAAATTCAACAATCGGTAACTTATTAGGAATATCAAAGTTGTTTTTAAATCAATATAAAAATCCAGTTGATGCAGGTTTTAATAATATGACTGATGCACAATTAGAAGGTATGAGAATATCACCATCATATGTTGATTTATCATCTTTTGGAATTAATTCTGGAGCGTTAGCACCAACAGTTAGTGAAGATGATCCAACATGAGTTTTTTATAACAATTCAAATAATCGTATTGTTGCAACAAAATTTACAACTAATTTTGGCGCTTCGAAATGAAGTGATGGTAAAGAAGTTAATGCACAAGATTTCATTGATGCAATTCAGTATATTTTAGACTTAAATACTGGTAGTCAATTACGTTCACACTTATTACGTTTTAACATCAAAGGAACAAACGCTTTTAATGATGCTCAAGAAGAATATATTCGTAAACATGGCGTTCCTTATAAAAACCCTTTTGGTCGTGCTCCATATATTTTAAATAAGCAAACAGGTCAATGAGAAGAAGATCCTAATTTTGTTCCATACCAAAACCAAACTTTTGATAAAAATGGTCACCCTGTTGATGATCTAGAAATTGCGGCAATTAAAAAAGCAGCTTATGAAATTGGATTAAGTACAGGTCAAGCCTATACTAATATTTCAAATAAAGAATTAAAAGAAGCTTTAAAATTACCTGAAAATAAGGATTTCAAATTTCATGAGTCAAAAACTTTAACAATTTTTAATCCTAAATATGATCATAAAAAACCAGATAAAAACACACCTGAAAAAATTGTTATTAATTTAATTGAAAACCCTTCGTTTAATCATCGTCAAAAGTTGGCAAGTGATGATTTACAAACTTTAGCTAATTCAAAAGATTTATTTAAATTTAAATCATTACCAACTTCACGTTTTGATTTACTAATGGAATTTGAGACTTATGCTCCAAAACCTTCATTTAGTGTTTTATTGACTGATATTTTAGGAAACCAAGCTTTACTACCAGTTAATCGTCGTTTTATTGAAACACATGGTGGAATTGAAAAATTCGGTTCTACTCTTGAAAATTTTGTTTGAAGCGGTCCATTTAATATTGCTGATGTTGTTTTAGGACCTCAAGGTTATATGACATTAGAAAAAAATAAAGATTATTTTTCAGCTAATTGAACAATTAGCGATAAGATTAAAATTTATTTTAATTCTAAAAATGAAGTTACATCACTTTGATTTGAACAAGGGATGATTTCATCAACTACTGTTCCACCATCATATCAATTAAAATTCTGAGCGCAAAGTAAAATGCGTGATTTAATGAAAAAACAAGCAGGGGCTGGAACGGTTGCAATGCAATTTAATTTAGATCGTCAATCACGTTATGATAGTTATCAAGAATATTTAAAAAATCCTAATAAGGACATTCCAATCCTAGATCCTGATTTACGACGTGCAATTGCTTTTGCTATTAACAGAGAAAACATATTAAAATTATCTGGTTGAACTTCATCTTTTCCAGTTACAACTTGAACATCATTTCAAAGAATTAAAGATTCAAAAGGGCGTAATTTAGAATATTGATTTGATAATAAACGTTTTGATAGTGAATACATTGATACTATTAAAGATGAAAAAACACATGAAACAAGAAAACAAAACCGTTCTTTTTTAATCCAAAATAATGATTTTAGTGACCACGTTGCTGCTAAAAATTCAACATTTGAAAATATTAACCGAAAAGATACTGGTTTTGATCCACAAGTTGCTAAATTTTATTTAGATAAATATAAGAAAAAACATCCAGATAAAAAACAAGTTGTTTTAAAATTTGTTTATCCTACCGAACCAGCTGATATTGGAAAAGCTGTAATTGCTATTAAAGATTTAATTGACCGTAATCTGAATGGTTATGTAAAATTAGAGTTACAAGCATTACCGCCAAACACATTTGTGACATTTGCAAATAGTGGTAAATTTGATATTTCTTATCAAAACTTTGATAAATATGCTGGTAGTACTTATGACTCAGCATTAGGATTATTTTTAACACCTGATGGAATTGATTTAAAAAATAATAAAAGCTCAGGTTATGAATTAAATCCAACAGGATCTTGAACGCCAAAAAACTTTTTTGATCGTTATAAAAATAAAGCAGAATTAGATTACGCTTTGAAACGTTTACATATTAGTGAAGAAGACGCTGATATTATTCGTGAATTGGTAACGCCAACAAATATTAAAACAAGTGAAGTTTATAATCCACAACACCTAACAAATAGAGCTGATTTACGTAAATTAAAATTAAAAGATAAAGCCATTCCAATGTTTGATGTTAATTATTCTGAATATGACGCTAAGATCATTCATGATTTAGAACACCAAATTAATAAATTAAATCAAAAAATTAAAAATGAGCCTACAAGTAGTTCGAAATCAACGTGAGAAAAAGAAGCTAATAATCTACAGAACGAAATTGATACAATGCAAGAACGTGAATTTTATTTATCAGAAGAGTTCTTAAATAAACACGATCCAAAACGTGAAAATAAACCGTTTTTACTAGTATTGCAAAATAATGTAACAGGTCAATTTACAACGCTACAAATTCAACCAGTTATCGATAAAAAAACAAATCAATATGTTCAAGCTACAAAAGAAGATTATGCAAAATATTATAATAAAGAAAATATTGATGAAATTAATTTCAAAAAACGATTTAAATACAAATTTTCTATTAAAAAATTACCAATTGGATCATATCAAGTAATCAACTTATCTTACATAAATGATTTAAAACCATTATTAAGTAAGTACTTAAGAAATGAGAATAATGAAATATCTGATAAAGTTAATAATGAAATTTATATGACAAAATTAGAAACAGTTGTTGATCAACGTAAAAGAATTATTGATTTCTTTTCAGGGAATCGTCCAGGTTGAGAAGATGATGATAGTATCTTTAGAACAATTTTAAATTTAGAAAAAATTTTACGTGATGAAGCAGCAATTGTGCCTATTATGGAAATTGATACAAACTGAACGATTTCATCACTTGGTGGTATGAAAAATAACTTTACATATTATTTACAATTTGCTTATGATTATCGAAGACCTCCACGTCCTGGTTTACCAACATCACCACAAGCAGAAGGGGATAATTAAAAAAGATTATGGATTTAATTTTTAAAAGATTAAAGTTTATTTCAATTCAAAATTGAGTTTTACTTTTATTTGCAGCTGCGTTATTTGTTTGTGTAGGAGCTTGAATTGGTCATGAATTAGTGCGTCATAATTTATTTGATGCATATACCCTAAATCATGAAAAAGAATTAATTAACATGTTAAATCCTAAAGATTTAGAAGTTTATTTATCACAAAAAGAACAAATTCGTTTATCAATTGGTTTTTTTGGCCTATTTTTAGTTTTATGAACATTAACATATTTAATTAGCTTAGTTGTTAATATTCGCATTTTAATTAAGTATTTAAATGATTTTAATGAAAATAAAACGAAAGTTAAAAAAGCTTTAATTATTTCATTAATTCCTTTCATACACTATGTCTCATCAATTTTATTAACAGTTGTGTATGATAAAGTATTTAATTGAAATCGAAAAAAAAGTCACACTTTACAAGAAGAATATATCTCGGCTTATTAAAATAATAAAAACAGGGTGAAGTTGCGCTTTTAGCACAAAACCCTGTTTTTCTAAATAAATAAAACATAAATACTATAGTATTTTTAACTTTATTTTTCAAAATAAAGTTAAACTTCTAAATAATTTTGATATTCTAAACTAGAATATTTGTGTCAAAAAATGAAGTGGATAATTTTTTAAAAAATACTATTGAATAAACATAAATAATATAACCATGAACTACAAAAAACAAGATAAAAATGTTTACAAACATTAATAATCTTGTTTTTTGTTTTTTAATCCTTTAAAATATTATTAACTATAAATTATTTATAGTGCTATTTTATTTGGGGATGTCACGGTTTCGACGTGACACATTAATTTTTAATTGCAGTGGGGTTAGCCCCTTATCGCTTTCGAGGCATTTTAAATGCAGAAAATAAAAAATCTTCTGAAGTAGAATTAAACCCAGCGTTTATGGCTTCAGCTACTAATGCAAACTACGCTTTTGCGTACTAATTAGTTATTAGTAGAAACGTTCATTAACATAATTACTATTGGTTGGTTTTTGGGCTTATTTTACAATAGTTTTAAATTTAAAATTCTTATTTGTTGTTTAAATTTAAATAGATTTAACAAATAGTTAGTTAATTTTAAATTTGTTTTATTAGTTATTAACTACACTATTTTTAATAAAACTAAACTGTAGATATTATTAATTATGTGTTGCGGAAAGGGGTTCGACTCCCCTCATCTCCACCATCACAATTTCGAGTAGAAATTTAATTATTATTAAAATTTGTTGTGCAATTCAATAAGTTGTGACCAAAAATCATGGTTAATAATTCCATGATTTTTTTATATTATTATAATTCTAAAGTCATATGAATAATGTCTTGACCATCATCTTCTAAAGTCATTTCTTTTTGAATTTTAAATCCCATTTTTAACCAAAATTCTCTAGCGCCTGGCAAGTGATGGTGTGTGTGTAAATAAAAACATTTAATTCCTTGTTTTTTAGCTTGGTCTTTTAATGCGTTAAACAAACGAGTTGCAATTCCTTGACGTCGAATCAAAGGATCAATATATAATTTACCAACCTCACTTGTTTTAGTACTTCTATCTAGATAGAATAGATTATTATATCTATAATCGTATTCATGACATGAAATTGTTCCAACTAATTCATGTGTATCTTCTAAAAAAACACTAAACCATATTCCTAAAGGATTAATAATGTAAGTTTGTTTAAAATTAACTAGTTCCTTAGGTAAGTTATTATGATCAACTTTGGGATAAAGTTTTTTACGGAAAGCAACAACATATGCTACGACGGTATTAATATCTTGTTCATTGATTTTTTCTATCAAAACCATAATTTAAAATCTAAAAATCCTATGCCATACTTAATAATTTAACTTTATTATACTGCTAAATTTGCTATAATTTTAACGTTTATTCAACAATAATTATTGAACTAAACACTCTTGCTAATTTTAAAACAACAAAATTAGCTATTAATATCCATAAGAGGAGTAAAATAAATGGCGAAATACGAAATTATGTTAGTTGTTCGTGGTGATTTAGATCAAGAACAAGCTAATAAAGTTGCAAATGAATTAAAAGCAACTTTAAAGAATACAGAAGTAAAAGAAAATAATTATGATGGCGTGCAACAATTAGCTTATGAGATTAATAAGTTAAAAACAGCTTATCGTTATGTTTATAATTTTGAAACTACTGATGTTAGCCTAATTAATGAATTTAGACGTTTAGCAATTATTAATAAAAATGTATTGAGACATATTATTATTAATTTAGAAAAAGATTACGGTTATAAAGCAACTGTTAATACTAAAAAAGTACAACGTAATGAAAAACGTGCTGAAGTTTATGCACGTCAAAAAGAAGAAGCAGAACGTCGTGCAGTAGAACGTCAAGCAGCTTATGAAGCAATGAAAGCCGAACGAGAAGCAGCTGGTTTGCCAGTTAAAGAATTTGTTAAAAACACAAATTCAAAACGTTAAAAATAACGTATTTTAATTTAACTTTAAAGATATTGAGGCGTAAAATGAATAAAGTAATATTGATTGGTAATTTAGTACGTGATCCAGAAGCTCGTCAAATACCAAGTGGGCGTTTAGTTACTAACTTTACAATCGCTGTAAATGACAATACACCTAATGCTAATGCAAATTTTATACGTTGTGTTGCTTGAAATAATCAGGCTAATTTCTTAACAACATATTTAAAAAAGGGTGATGCCATTGCAATTGAAGGACGTATAGTTTCTCGAAGTTATGTCGACAATAATGGTAAAACAAATTATGTAACTGAAGTTTACGCTGATCAAGTTCAATCATTATCACGTCGAAACCAAAGTCCTAGTGATAACAATAAGGTAAATGTTGATACAATGATGGAATCATATACAGGTATTAATACTGATGCAGCATTTAGTTCAAACAAACCTCAAACTACCCTTTCATCGACAACATCAAATTTAAATAAAAATAATGATGAAGAAGATGAAATTACAAGTTGAATTAATCTTGATGATGATTTAGAATAGGAGATTTTATGGCCAAAGTAACTAATAACCGTAATCGTAAACCACGTAAAAAAGTTTGTATTTTATCTGCTAAAGGAATCGAACATGTCGATTACAAAGATGTAGAATTACTACAACGTTTTATTAACAATAATAACAAAATTGCATCACGACGTGTAACAGGAGCAAGTGCTCGTATGCAAAGAAGAATTGCAAATGCAATTAAACGTGCACGTTTCGTAGGATTACTTCCATACGTTAAAGAATAATTAAAATCAAAATAAAACGAATGTGTCATTTTATAAATAATTAATGATATGTTCGTTTTATTTAAATAATAAGGAGTAAAGTTATGAAAGTTATTTTATTAGAAGATATTGCAAGTCTTGGTAAAAAAAATGAAATTATTGATGTTAGTGATGGTTATGCAAAAAATTTTTTAATTCGACAAAAAAAAGCAGTTGCATTAACAAATAAATCACAAGAAGTTTTAAATAAAGATCTTGCAATTTTAGAAGCTCAAGAACAACAAGCAATCTTAGACGCAACTTTACTAAAAGATAAACTAGAGCAAAAGCCTCTACAATTTTTTTTAAAAACAAATAATTTACAAACTTTTGGATCAATTAGTAATAAACAAATTATTGATGAAATTAATAAAGAACAAAGATTTATAACAAAGCATATGATAACAAAACCACATGCATTAGGTATTGGTGAACATATTGTTGAAATTTTATTGCATAAAAAGGTTACTGCTAAACTTCATGTTATTGTTAGCAAAGAATAATAATTATGCCAACAAAAGACTTAAATGATGCGATATTAGATTTGTTTTGTTTAATTATTAATAATAATGACTTTTGAAAAGATGTTGTTTTACGTTTAGAAGTTAAAGATTTTCCAGAAAAGATTCAGCAAAATATTTTTAATACAATTGCTAATTTAAACGAACAAAAGTATAAAATCAATGAATCAAATATTTTAAATGCATTAGCTAATTATGTGATTATTGATGAACAAGATCAAAATTATTTATTACATAAGAATTATTTACTTCAAATTTTAGATCGTACTGATTATTTAGTAGATTTAAAAGATTGTATTGAAATTATTAAGAATGCTTCAATTAAAAATAAACTTGATGTTTTTGCAAATGAGATTTTAGCAACCCAAATTTCATTAATTAACGCTAAAGATCAATTTAAAGAAATGCATGAAAAATTTTTAGAAATTCTTGCATCACGAACCGAAGATACTATTGAAAATATGGAATTAATTGCTAATCGATATTTTGAGAAATTAAATAAAATAGGCAATAGCGGGATTATTCCTGGAGTTATTAAAACTAAATACGATAATATTGATAAATTCACTAATGGTTATAAACCTGGAGAATTAGTTGTTATTGCCGCTCGTCCTGGAATTGGAAAAACTACATTTTGTTTAAATGTTATGGTTAACAATGTTAATGAAATTATTGAATATAACGAAAATATTCAACCAAATCAAAAAGAAAAGATCATTGTAATGTTTTCGCTTGAAATTACTAAAGAACAAATTTTACAAAAGTTTATCTCAATTAAAACAGGAATTTCTAATCGTGAAGTTATAGAAAATAAATATCGTATAACTAAAGGATATGACACAAGGTCTTTTGCTATGCAAGCAATCAATGATATTAAAAGTTGACCTATTTTTATCGATGATCGTCCTAATATTTCAATTGTAGACATTGAAGCAAAATTATATGATTTAAAAAAACGTTATGATATTGCTTTAGTCGTTTTAGATTATTTACAATTGGTTTCAGGAGGCAATGCAAATAAAAATATGACAAGAACACAAGAGGTAGGTCGTGTTTCAAGTGCATTAAAAATCATTGCTAAAGAAATTAATGCACCAGTAATCGCTATCGCTCAACTTTCACGTAAAGCAGAAGAACGTGATGTAAGCAATAATGTTAATATGAAAAATAACCCTTTAGTAAAAACAATTGATAATTCCCCAAAATTGTCTGATTTGCGAGAATCTGGATCAATTGAACAAGATGCTGACGTTGTTGCTTTTTTACATTGGGATCGAAAACAACGCAATTTTATGCAAAATGATAATCAAGAAACCCGAATGCGCGATGATTTGATTGAAGCAAAATTTATTGTTGAAAAGAACCGAAACGGGTCAACAGGTGAAACTGATATTATTTTTTCTAAATTAAATAGTAAATTTATTCGTGCAACCACTTCAAAGGAGTAAATATGAAAAAAAACAAAAGTAAAAAGCTAATTTTTTCAATATTAGGAATTAGTTTTATTGGATTAACGCCAATTGTTGCTGCATCGTGTTCAGCAGCTGCTCATCCTCAAGTTAATGTTCATTATGTTAGTGCTAATGCTACAACTAATAATATTGGTAAAATTAAAATTCGAGCCTTTGAAGGTAAGAATCAAAAACATGAAATGATTTTTGAGAATCAAAATTTATTAGATGATGTTTATTATGCGGCAGGTGATTTAACTTATACGAGTGTTAAAGATAATAAACGCTTTGATAAAAACAATAAAGAAATCGATCAATTTGGTTATTTGGTTCCCAAAAAAGAAGTTTGAACAAAAAGTTTATCAAGTTCAACTAAACTAACACCAATTGATTTGAGTATTTTGCAAAATGAATTGATTCGTTTAATTGGTAATAAGTTAAATTTTGATTCAACAAGTAAATATGCTAATAATTTAGAAATTCTTGACCAAACAAAAAAACAAACTGTTTTTAACCAAAAACTATATTCAAGTGATGAATTAACAAAAATAAATAAGGTTAGTGAATTAATCAACTCTTTAGTTATTAGTGATGGATTGTATACTTATATTACCTCTATGACAGATCAATTAATAAAATGATTAGCAGAACATGCATTTAATAACCATTCAAATAAAGATTTTGAACTTTTTAAAGGGTTAAAAAACGAAGTTGCTAAAGAATTTTTATTAGCTTTAGTACAAGGTGTAGGTGCAGGTAAACATACATACAAACTTGCTTTATATAATTTTAGTTTTGATTGAGAATATGTAGATGCAATTACAGGTTTAAGTATTAATAAACCTAATTTTGGTGTTAAAGTAAATGATGAATTATTAGTTAATCCAAGTTTAAAAAATGTTCTTGTACGTTTAAAAAATATTAAATTACAATATGCTTGATATAACTCTTCAAACAAAAAAGCTGGATCATTTATGGAAGCTAGTTCTAATGGTAGTCAACTAAATGCACTTAGTTCAATTAACGCTGCTTTATCAAGTGTTGATAAAAAAACTTATGCTGAAATTCATGATGATCACAAACCAGAAATTCGTAATTTAGTTTATGAAATTCCATTAAAAGATATGGTAGTTAATTTTGCACCATCAACAATGCGTTCTGCTAATATTAATCAAAAAGCAACACAAACTCTTCAAGAAAATTTTTTAAAAATTAAAAATCAAAAAACAGGGTTAACTGAGACAGAGAAAAAACGTACAACTGTTTATAATGAATACTACACAGGTACATTATCTAAAATTCATGCGTTTAGTATTTTGGGCCGCGATTTAAAACAAGAATTAAAAAACGAAGCTAAAGTTAATAAAAAAGAGTATGTTGAAGATAAAAAACTTGAAGACTGACAATATTATGAACATGATCGTGGTCTAAATGGTGTTTATCCATATGCTTTCTTAGGTGAAAAAAAGATCAATGAATTAAAAATAGATGATTTCATCAAATCACGCAAATTTGTTGAAGCAATTAAAGCATCTAACAATTCTCGTACTATTTTACCTGTTACTAACCCTGAAAACAACCAAATTTTATCAAAACAATCATATTCGCAATTAGCAATGATTGCTTTAGAAAATAAGGATGCTAATATTCATAATTTGACTAGTTTTAAAGCTGCTAAGAATAATTTTAGAAAATATCTTTTTAACCTTAATGAAATTAAAAGTGTAAATGTTAACTATAGCAAATGAAATGAAAAGAATTCATAATTAATGAAACTAAACAAAGTTATTTAAAAAATATTATTAAAAAAATCAACAACATCGAGAATCATCAAGTAGTTTTTCCTTTAAAAAAACAACGGTTTAGATGTTTTGATTTTTTTGATATTGAACAAACTAAAGTTGTTATTTTAGGGCAAGATCCATACCATACACCTAAAATAGCTAATGGTTTGTGTTTTAGTGTGGATTTAGGAAATAATTTACCAGGATCACTAATCAATATTTTTAAAGCTTTAGAATATGATTTACAAATAAAAAGAACAAACCCTGATTTGTCTGATTGGGCAAAACAAGGAGTTTTATTGTTGAATACTGTTTTAACTGTTAACGCTCATCAACCAAATTCGCATAAAAATTTTGGTTATGAAGAATTAATTAAAAATGTGTTTAACGAACTTAGAAAACAAAAGCATGTTGTTTATTTATTATGAGGAAAACAAGCTATGAGTTACATTAATTTGATTGATCAAAAACAAAATTTAATTTTATGTGCTTCACATCCTTCGCCATTAAGTGCACATCGTGGTTTTTTAACTTGTAAACATTTTAGTAAATGCAATGATTATTTAATTAAACATTTACGCACTCCAATAAAATGATAAAATTTATTAATTAGTGAGGTGAATCTGTGACAAATATAATGAGTGTATTACATACAACTAATACTTCACAAGGTAATGGAATTAATTCATGACAATCAATTTTAATTTTTTTAGCATTTTTGATTGTTTTTATTATCTTTACAGTTATTAAAAAAATCTATTATTCAATTCGTTTTCAAAAGCGTTTTTATATTATTCCTAAAACTTCGGTTAAAGGGATTTCAAATATTGCTATGGTGATTTCGATTTCTGTTGCAGTGATTATATTATTAACAGTTTTATCAGCTAACACAGCTTCTGTAATTTTTCGTGCTTGACCAGGTACACGTGTTACTTTAGAAGGGATTCTTATTAAAATTGGAGGTTTATTATTTGGTCCAATTTTAGGTATTTTTATTGGTGCAATGACTGATTTATTATCTGTGGCTATGACTGCTGGTGTATTTCATTATGGTTATTTAATTGCTGCTATGGCTTATGGATTAATTGGAGGACTAATTCGAATTATTCTAACTACTTCCAAAAAAAAGGATTTACCATTTGCAATTTATAGTTCAATGGCAACTATTTTAATTGGTGTAGGAATTGCCTTATTTTTATATTTTGCTCCAGGAATTGGTGATAAGGGTTTTAATATTCAATTATTTGGTTTTGATATTAAAATTGCACGAACATTGATGATTGGCATTATTTTAGGTTTTTTCTTATTATCAATTATCGTTGTTTGGTTTAGTTTATTGATAAAATATTGATTCAATAAAAAAAATAAAGCTAAAACCAAAACAAATTGATTCATTATATTTGCCCCTGTTTTAGTAACAATTTTACTAACTGAAGCAGTGGTTAATGTACTAATGATGCCATCGTTTGATGCTGAATTATCAAGTCTAAAATATACACAATGATTATCAATTCGTGCTGTTTTATTTATTCCAATGGTGGTTTTAAACTTATTGATTATTTATCCAATTTTTAAAATTGTTGTTCCTTTAATTCGTTATGATTATGAAGATGATATTGTTGAGGATAAACATATTCCAATTCATGTTGACTAATAAAGAAAAGAGAAATTAAGATGAGTTATAATTTACAAAAATTAATGTCATCATGCATGTTTATATTAACTGATGATGAAATTAAACAGGTTGAAAATCGTTTAAATAATTTATTTAATGAAATTAAAGTTTTTGAGCTTTTTAATTTACAAAATATAACACCTTTTGAAATTATTAATAAATCCTTTGATAATTTTTTACGAGAAGATGAAATTAATTCTTGTGTAGGTAATAATAGCGAAGAAGTTCTAAATAATTGTCTAAAAGTAATCGATCATTATGGGGTATTAAAAAATGAAAAATAAAACTATTTTAGCGCTTCATAATCAAATTAAAAAACGTGAAGTTAAAATTTATGATTTGATTAAAAAATCGATTGATCATGATTTAAAGATTAAAAATTTAAATGCAACTTTAGCAAATAATTATGAACAGGCATTGAAACAAGCAGCTTTTTTAGATGAAAAGATTAGTAGTGATAATTTAGAAATTGATTATTTATTTGGAATTCCATATTCTTTAAAAGACAACATTTCAACTAAGAATATTATTACAACAGGTGGTTCATTGTTTTTAAAAAATTACAATCCTCCATATGATGCGACAGTTAAAACATTATTAGATCAAAAACAAGCAATTTTGTTAAATAAATCAAATTTAGATGAATTTGGTTTAGGAGGCACTGGTACTGATTCAGCATTTGGCCCTGTAATTAATCCATTAAATCCAAAACATGTTAGCGGAGGTTCTTCATCTGGTAGTGCTGTTTTAGTTCAACAAGGCGTTGTACCTTTTGCAATTGCTACAGATACAGGAGATTCTGTTCGTCGCCCCGCTTCAATTATGGGTATTGTTGGTTTTAAACCGTCATATGGTCTTATTTCGCGTTATGGCGTTTACCCCTTTGCTCCTTCTCTTGATCATGTTGGAATTTTTACAAATAATATTTTTGATACGCAAATTGTTTTTAATGCTCTTGCTAAGCGAGATTTTAAAGATTTTACAAGTCTTAGTTTTAAAACACAATCACTTTCAATGCTAAAAACAAAAAATAAATATAAGATTGGTGTTTTAAAACCTAGTTTTGATTTATTAGCATCAAAATATCAACAAGAATTAACTAATCTGATTGTTAAATTAAATGATGAGCATGAAATAATTAATGTTGATTATGATATTAATTTATTAAAGGCAATTAGTCCAGTTTATAAATTAATCGCTTTTAGTGAGGCTTATAGTTCATACTCTAATTTTACAAACATCACTTTTGGCAAACATGATATTGATTATGATAACTATGAGGATTTAATTATTAAAACGCGAACTTTATATTTAGGAACACAATTAAAACAACGTTTTATGATAGGTGCTTATGTTACTCAAGAAAGCCAGTTTGAAACTTTATTAGTTGCTGCTAAAAAAATGCGTACATTAATAGTTGATCAAGCTAAAAATTTATTTAAAAATTGTGATTTAATTTTAAATTTAGCAGTTCATGATATAAGTGAAACAATTGATGATGTTAACAACCAAAAGCCCACAACTAATTTAGTTGAAGATGTAATGCAAATTGCTAATTTTGGTGGTTTTCCATCAATTGTAATCCCCTTTTTAACAACTAAATTAGGAAATTTAGGTTTAAATTTGTGAGCAGATTACTTAAATGATAATAAATTATTAAATGGCGCTTATTTAATCAATAATTTACTAGGAAATGAGGAAAAATAATAATGCAAAATTTTGAAGTTATTATTGGTATTGAAGTGCATACTGCACTTAATACAAAAACCAAAATGTTTTCTAATGCAGCAACATCGCATAAGAGTATTCCTAACACTTTAATTAATGAAATTGATTTAGCATTGCCAGGTACATTACCAACTGTTAATCAAGAAGTTGTTCATAAAGGACTTTTTTTAGCAAATGCATTACACATGCGTACTAATCATCAATTTATTGCTTTTGATCGTAAGCATTATTATTATTTAGACTTACCTAAAGGATATCAAATTACACAAAATTATTTTCCAATAGGTCAGAATGGTTATATTCAAATCATTGATGAATATAATAATTTAAAACGAATACGTATCAAACAAATTCATTTAGAAGAAGATACAGCCAAACAAACTAATATAGGTAATCAAATATATTTAGATTATAACCGTGCAGGATGACCTTTAATTGAAATCGTTAGTGAAGCAGATTTACGAAGTGCACAAGAGACGGTTTTATTTTTAGAAGAATTGCGAAAAATTTTATTGTTTAATGATATATCAGATGCAAAAATGGAAGATGGATCATTACGTGTTGATGTAAACGTTTCAATTAGACCTCAAGGGGCTAAAAAATTTGGCACAAAAGTAGAAATTAAAAATATTAATTCTATTTCAAATGTTGCTAAAGCTATTGATTATGAGATAAGACGTCAATTAAATTTAATTTTACTAAATCAGAATGTTGAACAACAAACACGACGTTTTGATGATAATACGAATACAACGGTTTTTATGCGTTCAAAAAATGATGCTATTAATTATCGCTATATTCGTGAAGCAAATATTGCACCAATTCATCTAAGTGATGATTATGTAAAAAAATTGTTTTTAACTAAATCATGTAGTATTAATGATTTGCGTCAACAACTAGCACAAAAAGGTTTAGTTAGTTCTGCTATCGAACAGTTATTAAGTGATGGACCGTTGTTTAAAGCATTTAAATATGTAGACAAAATTGTTAATAATCCTTTAAGTGTTTATAAATGATTATGTTTGGAATTTATTGGTCTAATTAATAAAAATACGCAAAACATTGAAGAAATTACACCAGAATTATTGCAAAAAATTGCTAGAATGATCTTATTATTTGATCAAACTTTGATTAATGGAAAACAAACAAAAATAATTTTAGAAAAAATTTATTTGACAAATAAAGATCCTCAAATTCTAATTAAGGAATTAGGTTTTGAGCAAATTACTAATGAAAATGAAATTACTAAACTTTGACATCAAATTTTAGCTAAGAATCAAGAAATGTTATTACAATACAATGAACGCCCCGACCGTGTTGAAAAATTTTTTATGGGTGAAATAATGAAATTAACTAAAGCACAAGCGAATCCAACCATTTCTTTTAATGTTTTAAAAAAAATCTTACAAAAATAAAAACAAGCAATTAACATTAAAAAAAAGACGGTCTTTACGGCCGTCTTTTTAATTATTAAATTGGTTTAAAGCTTTTGGTAAATTTAGTTTTTTTGTTTTATATAACATTTGTAATCAAGCAAATAATAATACAATCCCGACAAAACCATAACTAATTACAAAGATTCATCAAGGGATAGAAGCTACTAAAACAATATTAAAAGCAGCAAAGATTGCTAATTTATAAATATGAATAATTCCAAAAGAAAGCGGTGTAGCAATAAGAATATTTAATAATCATACGATTGGATAAATAAACATAAAATTATTAACATTTCTAAAATCACGCATTCCTAATACTTTCATAATAGCAATTAAACGCCGTGATTCATTAGCTAGTGTTGAAGCTACTAGAATAATAATAATGACAAGTGATGGAATAATAGCAGTAATGATATTTAATAATAATGTAAATGATGTTTTATCAACTACTGAAGCAAATATTTCGTTTTCAATATTAGCATCAACTGCTTTAAATGATGGTAAATTAGCTTCTTTTCCAAAAATTTTTCCTATTTTCTTTAATAATTTATCTAAATTAATTTCTGAAAAAGGTTCTCAAGTAATTTTATTTTTATCGAAACTAGCATTTAAAATCGAATTAGCTAAATCTCAACTGTAATAAAAAATTCAGTAAATATTAATGCTTTGAACATTACCACCAATATTTTCTAATAAATTTTGTGGTCATGAATCAATTGCTGGTGATAGTCCAGTAGGTGAATAAAATGAAGCCATATTATTAAAGAAAATAGGTTCTTTTTCTTTTGTAAAGAATCCATTGAACTCGCGTTCTTCAATGATTGGATAACCATTTTCGATTTGAATCCGACGGCGATAATCATCAAAACCAATTAGTTTATTTGCAACTTTTTGATTAATAATTAATTGCTCATCCATTAAACTATCATTAATCCCAACAACTTTAAACTTAGTTTTATAATTCTCTGGTGTGTTATTAAATTTATTTGATAGACGTTTGATGTGATTATTAATTTGATATTCAAAAACATCATTAATCTTTAAATGATATTTTTGGGCGACAACTTTATTAATAATAATGTTGTGAATCTCATTATTAGGATCATCATTTTTAATTAGATCAATTAAATTAACACCCTGATCATTTATCAAACTTAGTTGAGTAGTAGGACTTGGTTTTCCATTAATAAATTTATTTTTTTCATAATTAATACCCAATATTTTAATTTCATTAGCAAATCTTTTTGTTTTTGTTTGCATTTTACCTAATAAGTATGTATATGGTTGTTGTACTTTTTCATCTTCAATATTAACAAATTTTAAACCGGCTTTATATTGTAAATAACGAGTTGTTTTGAAGGTGTACATTTGAGTAATTAAACGTAAAAATTCTGGTAAGAATTCTGCGGCGCCCTGCTCTGTTAACACGCCTGGTTTTTCATTGATTTTCCATTGCCCATTTTGTTTTATAAAGAAAGCATTTTCATCATTTTCACTTTTAGGTGGTAAACCATTTTTTGTTCATAATGATATTTGATGAATAATACCATTATCTTCAAAGTTAGGAACATCATATAATTTATTGATTTTTTTACCATTAATAATTTCATAACCAAAAGTTTTAAATGCTTCATCTAATAAGTGTTTTTCATTTAGTTCTGATCGAGCTATTGCTGTGATAGGTAAGAAAGTTTTTGCAAAAACTCAAGGGTTAAATTCAATGTTAACATCCGTTGCTTTAACACTAATATTTCCATCTAAAGAACTTTTTGAAATTACTTTACTATCAAAAAAACGAATATTTTGTAAGATTTCAGGTTGGGTACTAATTGATGGATAATAAACATTTGATAACATTTTGCCATAGACTGGATGATCAGTACCTAAATTTTTAATTTCACGCATTGCGTTATTGTATTTTAGAGACGAATAAGGTAAGCCATTATCTTTTGTTTTATCAATAGAATTAACGTTATCTAAGTATAAGTTTGTATCACTACCCTCTAGTGTTGCTGTACTAATTTGATAAACATAACCCTTTTCTAATTCATTATAATCTAAAATTGCAGTATCAGGTTCAACTAAAGAAGCCATTGCTTTTGTCATTATAAAAGGTATTTTTTTCTTATTAACAAATTCATTACCCATTGCTTTTAAAGTTAAATAAAGATTACGATTAGGAGTTAAGTAATCAATATTAATTTGCTTTTGATTATTATTGTTTAAACCGTAATAATTGTTATCAAAATATAAAATATTATTATTAAATTCATGTTGTTTATTATCATAAATTAATAAATCACTATTTGCGATTCGCATATCGCGTAATTGATAACTTTGTTGGGTATTAATTTTTAATTTTGTTAAGTCAAAAACTACTTCTTTTTCTTTATTAACAATACCATAAATTCGAAATTCATCATTTTGGTTAATTGTATAAGTCAAATAAACTTTTTGGTTAATTAGATTTTGTGGTAAATGATTAAAGTATAAATATGGTAATCTTCCATAATAATCATATAAATAACGTCCATTTTTGGAATAATCCAAATTTTTTAAATCATTACTTATATTATTTAAATCAACTCATGTATTTCCTTTATGGTTTATAACATTAACTAATTGATATTTTTGTTTTTGTTTTAATTGTGAAGGATCAAATACCAAGTATTGACCTAGAGTTTTTGCTTTTAAATAAGTATGTTGATTATTAGCATCAACATAATCTAAAATTACATCATTGTCATTAAAGTATGATTTTAGTAAATAGATATATTGTTTTTGATTAGAGTAAATAAAATGGCCTATCTTTCGATTATCTATATTTAATAATGATGGATTTAAATTTTGTGAATTAATTAAAACTCTATTTGTTTCTTGTTCATAAATACCTTCAAATTGAAAACTACCTTTAACAAGCTGATTAGTTTTAAAAGTGATTATTCCCTTATCATTAATAAGTGCTTCAATAATTACTTTTTGCTTTGTTTGATTATTGATATATACAACATTGACTTTTTTATTAACTAAATTTTTAATTTTAATTACAATCTTAACATTTTTAACAATTTGTTTTAAAGGAGCATATTCATTAGAAACACCCATTTCATCAAACTTTGAAATGTAATAAAATCCTCCCTGTTCTGTTGGTGAATATAAATCAACTGCATATTGATATTCACGTTTAGCATTAGTAATACTTGCTGACTGACTAAAATTATTTTTTGTTGTTGCAATAATAGAAGCAATAATAGAAAAAGAGGAAGCGATTACTAATAAAATCAACATTCTTGCTAAATTGGCAACAACATAAGTAGCTTTTAAAACTCGAATTGCACCAAATTTTAAAAAGATTGCTTTTGTTTTAATGATAAATTTATTGATTTTAAAACCTGCATCGCCTTTCATTGTCTCTGCGGTTGAATGTTTTAAAATATAATAAATTAACACCCAGCAAATAAAGCCTAAAATTAATGTTAAAGCAATTGGAATAGCAAAAATTCAACCAACACTAACAAATTGGTCTGGGATAGGAATTAATCAATATGAGCTAAATAAACGCATAATTAAGGGTTGAATTTCATAAGATATACTATAACCAATAATTAAAACAATTAAAGAAGGAATTGTATAAAAAGGAGCGAATGAAAATCAAACTTGTCATCTTGATACGCCTTGAGCTTGCATAGCACCGAACATGACTTTGTTTTTATTAATCATATTTTTAATACTTGAGGTAACAAAGTAGATTCCTAACAATAAGATTAAAACAATCAAACTAAGTGCAATTGCAATAATTAAATTCCTCAATTGTGTTAAAAAAAAGGTTCGTTTAACATAAATTGGATAAGGGTTATCCTTATCAGTTCTCTTATATATGTTACCTTTTTTAGAATCTAATAATTCTGATTTTATTTCATTATAAACATTATTTTTTTGTAATAAATTAAGATCATTAGTATTAACTTTTAGTGTGTAATACTCTAAAATTGGCACGCTTGCTGAAATAGTTTTAAGTTTAGCAAAACCTGAATCATTAACATATAAAACAGTTGATTTTTGTTTATTAATTAAGATGTCTGAGTCATTTATTGCTGGAAATAAAAATTCAGGACTATCTACAGCACCAATAATAAAGAAGAATTGGCCGTTAATTTCAATTTTATTAGCGGGGCTAACAACATAATTAATTCAACTATAATAATCTTTAGCAATTCTTTTTTTACGACCGTTTGGCAAAGTAATTTCACGATAGTTTGGTTGATCATCGATTATACGATATGGAAGTTTTAGTGCTTGTAAAAAATCTTCATAATTACCAATTTCTTTATAATTATTTATATTTTTTGTTGTTCTTTGATTAGCTTTAATATAAGATGTGCTTACGTATGCTAGATCTGAACTACGATTAATAATATTAATTGTAAACGGCAATAACAAAGCTTTTGATAAAACGTTTTGTGCTTGAATTGTATATCCATAAGGTGATTGCTGAAGTCGTAGAAAATAATAACGTTTAATGATTTTTGATAATCAAATTTTAAACATAGCATAATCTTGTGGATTATTTAAAGCTCACTGATCATAAACTGATTGCTGGTTTGTATTAATATCTTTAATACCTTTTTTAAAATTAATAATCTTTGCATAATCATTTAAATCTTGATCTAAAACATTAACTAAATTGTTTTTTTGATTTATTTGATTTTGTTTAATAATATTAATTAATTTTAAACGTGTTGGCAGATTTTTATTATATTTAACATTAATATAAGCTCGTTGATTTTTAACATAGCCGTTAAAATAATATTCTTTTTGGTCGCTATTACTATCTGCATTTTTATAAATAGCAACCACTTGTTCGCCATCTTGTCAATCTTTATGTTTTAAATTAAAAATCCATTCTTTATCTTGATATGGTTCAAAAATTTGTAGTAAATATTTTTTATAATCACTCATTTCTTTTAGATATTGAATTGATAATAATCATTCAGATTGATTAAATAAGATATTAAAAAATTGTGGAAGTTGATATATATTAATTAGTGATGTATTAATTCAAGGAGTATAATCACGCTCTTCAACTAGTAAATCTTTATCAGTATCTGGATCTTTTTCATAACTATTTTTTAAAATTTGAATGACTTTAATGTAATTTGAATAAAGGTCATAAAAGTTATTAATTTTATTTGAATTATCATCATTAATAAAAATTCGCTTAAATTTTTTATTTGGATTTTGACTATTTTTATTTTTTTCATTAATTTGCACCATATTATCAATTCATTGATTTCTTGGTGCAATTAAACCGTTTTGTGTATATGTTGGATTGCTTGCATTTGTGACAATAAAATTTGAATCACTTTTAACATCATTATAAATTGCTGATTCATGTTTGCGATAATAAATATTATAATTATTTAAAATCGAACGGAAATCTTGTTTAATTAAATCATTATATAAAAAGTGTTTAAATTTTGCTTGATCGAGATTTGCTGCTAATACTTTTTGTTCATTTTTTAATTGAATAAGTTCGTTATTAGAATAATTCGAATTTTGTAGAGCAATATTTATTTGTTTTAATTGCATCTCATAATCACGAATCTTATTTTTATTTTCAAGAGCTGATTTTTGTGAGTTTTCATAAAAAATATTTGTTTCTTCACGAATTTTAGCAATTAACAAGGCTTTGGCTTTTGCAATAATTTTTTGCACTTCTTCTATTTTTTTTGGATCGTCAATTAATTCTTTTGGAATTAAAACTTGGTTTTGAAATAAATCTAAATACTTCTGTTTATAGTTATCGATTTTTAATTCATTTATATAACGGTTTTTATCAATACCTTCATTTTTTAAATAATTAGCAAATAGTAGGTATTTTGTTGATAGTGTGTTAAAATCAACATGTAAATTCACACTATAAAAATCTTTATTTATCGTGTTAGTTTCAAGTGTATCAACAATGGCTTGAAATGTGCTTTGATAGTCTTTAAAATCAATTGATTGATTTGAACGATCGTTTAGTGTTTGAACAACTTGTTTTGAATAAAGATCTATATTTTTAATTAATACAGGATTTGTAAAATAAGATTTATTCTTAAATCCTAATTCTTTATATTTATCAAAGTTAAAAAAAGATTTAAATGGTTTATTTTTTTGGACAATTGCAGCATTAATTTTAAATGTTGTTTCATAATTACCGTTTGCTGATTTTAACGCCGGTGTTTTAAATAAAATATGCCCACCATCTAAATTTTGAAATCCTACTAATTGATTTTTAGAATTATGATATTCATAAACCAAATCATCATAATTGTTAGCAAAATTTTGATTATTTTCTTTATTGTTTTCAAATCAAAAAACAGTTTGTAATTTTAATTGATTAATTTTATTTCAATCGATATTTCCTTCAAGATTTAGTACATCTATTTTTTGATTGTTTACATTAGTTGGCCAAAAAAGGTTATGATTATGAAATGCATTATCAACTAGTTGTAAATAATTATTTGAATTTTTTAAAATATTTTGATTAAAAGTTGCTAATTGAATTAATTTTTCATTATGTTTTACAAGATTGTTGCTAACGTCAATTTCGTTGAAATTAATATTATCTTGCTCTTTTGTATCAATTAAAAAATCACTTTGTAAACCAATTTTTTTAATATCGCTTTGTGATTTTTGCAAACGTTGTACTGTAATCGTTGTTGCAACAATAACAACTGTTGAAACAATCATTAAAATTGAAAGTTGAGTTACTAAAGTTTTATTTTTAAAGAATAAACGCATCATATTCTTCATTAATAGAAACATTTTTTTCATATTAACTCCAAACTAAAAACAAATGTACAACTTGTGTTTATTTAAATCAGTATAATTTTATTATTATACCATCCTTCACCATTAAAACCAAGTATTAATAAAGTGATAGTAATTTTAAATTTTAATTGATTTTAAGTAAGTTGTATAGTATAATTTGATACGTGTAGTAACTTACTTACACAATTTATTAGCATGTGGAAGAGTTTTAAACTTGTTAAACCACGTAGCGAATATCTTAAATTTTAACTTAACAAATTAAGTAGAAAGAGGTGTTGCGTGTGGCTCCTAAAAAGAAAGAAGTTACAAGAATCGCAAAGTTAAATCTAATTGGTGGACAAGCTAAACCTGGTCCAGCATTAGCATCAGTTGGTATTAATATGGCTGAGTTTACTAAGTCATTTAATGATAAAACTAAAGACCAAAACGGTAAGGTTATCCCAGTAATTATTACAGCTTATAAAGATAAATCATTTGATTATGTTATCAAAACTACTCCCGTGACTTTTTTACTAAAAGATGTTGCTAAAATCAAAAGTGGTGCCAAAGATCCTAAAAAGCAAACTGTTGCAACAATTTCAAAAGAACAAGCTTTAGAAATAGCTCGTTATAAATTGATTGATATGACAGCATATGATGAAGAAGCCGCTTTAAGAATGATTGCTGGTTCTGCAAAACAAATGGGAATTGTAATTGAAGGCGTAAGTGCATATAAAGAAAAGAAAGGTAACTAATCATGGCTAAAATTTCTAAAAAATTATTTGCTGCTTATGAAGGAATTGATAAGCAAAAAGCTTACCCATTATTTGATGCAATTAAATTAGCACAAGAGAAGTCAATTACTAAATTTGATGGATCAATTAATATCGCTATTAAATTGAATTTAGATACTACTAAAGTTGAACAACAATTACGTGGGTCAATTTCATTGCCAAACGGTAATGGTAAAAATGTACGAGTTCTTGTTTTAAGTGAAGATATTACCAAAGAAGAGGCTGCTTCAGTTGGTGCTGATTATTTTGGTGGAGCAGATTACATTCAAAATATTGAAAAGATGTTAAACCAAATTGATGTAATTATTACTAACCAAAAAATGATGCCATTATTAGCTAAATTAGGAAAAGTATTGGGGCCTCGTGGACTAATGCCTAATCCAAAAATTGGAACAGTTACAAATGATGTTCTAAAAGCTGTAGAAGAATTTAAAAGAGGTCGAATTGAATACCGTACAGATACTTATGGAAATATCCATATGAGTATTGGTCGTGTTTCATTTGAAACAACTAAAATTGAAGAAAATGCTAATGCATTATTAAACTTAATTAAATCAAAAAAACCAGCGACTGTAAAGGGTCAATATATTCAAAATATTGCTGTTTCACCTACAATGGGTCCTGGAATAAAAGTTGTTATTAATAATAATTAAATTTTTTATTTATAATAAAACATGTGAAAGGTGTTAGTTATCTAACACCTTTTATTTACCTATTTTTAATTGCCTTTATATATGTTTTATTAAAAAACATAATATAATATATAAATATCTTCTTTAAGGAGTTGATTAAATGAGTCATAATGATAAAAATACTCATAATTATCAAATTTCATCATTAGATAAGATTATTAATGATTCAAGTGTTGCCATTGAATTTGAAAATGTTTATTTTGCTTATACAGAAGAACGAATGATTTTAAAAAATGTTTCATTTACAATTAATGACAATGAATATGTTTGTGTTATTGGTCATAATGGTTCTGGAAAATCAACGATATCTAAGGTTTTAACAGGATTATTAAAACCTAAAAGCGGTGTAATTAAACTTTTTGGAATTGAGATAAGTGCTGCTAATTTAAAATATTTGCGAAATAATATCGGAATTGTTTTTCAGAATCCTGATAATCAATTTGTAGGTATTACGGCTGAAGATGATATTGCTTTTGGTTTAGAAAATCGTAAGGTTCCGCCAAATAAAATGTGAGATATTATTAATGATGCAGCTGTTGCAACGGGAATTGAAGATCTATTAAAAAAAGAATCACTTGAACTATCTGGCGGTCAAAAACAAAGAGTTGCAATTGCTTCTGTTTTAGCAATTAATCCTAAAGTGATTATTTTTGATGAATCCACAAGTATGTTAGATCCCAAAGGAAAAAATGAATTAAAAGAATTAATGGTTTCATTGCGTGATGTTGCAAAAAAAACAATTATTTCTATTACTCATGATATGGAAGAAGTAGTTAAAGCTGATAAAGTTATTGTCATGTCAAATGGCGAAGTTCAATATATAGGTACACCACAAGAAATTTTTGCTAATGAAGAACGTTTATTAAAAATGCAATTAGATATTCCATTTACATTAAAATTAGCAAAAACCTTAAAGGAAAAGGGGTTAAAAATTGATTTAACTTTGAATAATGAGGAGTTAATTGAAAAAATATGCAAAAATTAATGGATTATTTTTTCAAAAGAAAACTTAAAGTACCACGACCAATATCTAATGATATTAGTGTTCGTGTAAAAAATTTATATGCTGTTTATGATGAAAAACAAGAAAATCAATTAGTCGCATTAAATAATATTTCGTATGATTTTAAAAAAAATAAAATCTATTTTATTATTGGAAATTCTGGTTCTGGTAAATCTACGCTAGTTACTCATTTTAATGGATTAATGATCTCGCGTTATGGTTTCGTTCAAGTCGGTGATATTGTTTCTGGTGATCATTTTGATTTTGAACGCCAATTATTAGGAGTGATTGATTCATATGATAAAAAAATTATTAATTTATTATGACGGAATCAACTAGATCAATGGACCTTTCTAGTTTTATTTTCTAATGAAGTTAATATTCAACAAGCACGGATTTTGTTTGAAGCAAATTTTAAACAAAAACCAATTAGTTTAAAATTTATTAAAACAAAAAATAATCATGAATTAATTACAAATCCTTATGTAAGAGAAAACGCTAAAATAGCAGTAGTTCGTGTTGATAAAAATGTTATATTAGAAATTAATGATAAGATGAATTATGAAGAGTTACAACGTTTTGAATTTATTAAAAAAGAAATTAAGACGAACTATCATTTATCAAAAAAAATAAAGCGGTTTAAAGAATTACGTAGACGTGTTGGTTTTGTATTCCAATTCCCTGAATACCAATTGTTTAAGGATACTATTGAAAAAGACATCATGTTTGGTCCAGTTAATTTAGGTGTAAAAAAAAGCGAAGCTAAAAAACGAGCTAAATTTTATTTAAATAAATTAGGTTTAGGAGATGATTATTTAGAACGCTCACCTTTTGGATTATCTGGAGGCCAAAAACGCCGAGTAGCAATTGCTGGAATTTTAGCAATCCAAAATGATATTTTAGTTTTTGATGAACCAACAGCTGGTTTAGATCCTGCAGGTGAACACGAAATGATGCAAATTATTTTAGATGCGAAAGCAAATAATAAAACAGTTTTTGTTATTACCCATACTATGGAACATGTGCTTGAAGTTGCTGATGAAGTGATTGTAATGGATGAAGGTGAAATTATTAAAACAGGGACACCTTATGAAATTTTTTTTGATCAACACATTATTAATTCAACTTCAATTCAAGTTCCTCGTGTAATTGCAGTTATTAATGAATTGATTAAAAAAGATCTTAAATATGAAATTCTAAAACAAAAACAACCACGCACAATTGAAGAGTTAGCAGATGCTATCATCGAATTTAAAAAAGGAGAAAAATAAATGAGTGCTAATGCTTATGTGTTTCGACGTTCACCAATTCATCGTTTAAATCCAGCAATTAAATTTATTTCTTTTATTTTATTAATTGCTATGATTTTTTTACCACTTGGCTTTTTTGCGCAAATGATTATTGGAATTTTTATTTTAATCGTCTTTTTTGTAGCTAAATTACCTAAGCGTACTTTATGAAATGTTTTTAAATCCGTCGTTATGTTGTTCATTATCCTACTACTTATTAATTGAATGACATATAAAGATCCTATTGCTATTTATAATGTCACAGATCAAGCTAAAGTAATTTTAGGTAATAATAGTTGAATTAATGGTCCTATTAATAGAAATTTATCATTTAATTTATTTTATAATGATATTTCATCAACTCATCCCCAAAATTTAGTTTCGAATATTTGAGGTGGAGAAATTAAAAATTATATTAGTCCTGAGATTATTAAAAAACTAATTGATAAACCCGATTATAATGTTGCTAAATTTTTAAATGAAAATAACGTAACTGTTAAAAAATTAGAAAGTACTTTTAATGCGTTAAACCAAGATGTTCGATTGAATAATTTTTATCCAATTTATGGTAATAGTACCTTAAAATCTGGAAAACTTGAAGTGCCATTAAGTCATTTATCATATTATATGTCAACGAATTTATGAAAGATCAATGGTATTAAATATCAAGGTTTAATTTTGTCAGGTGTAGGTGATCAATTAGGTAAGGCTCAAACCGCTTTATTTTATACACGAAGTCCTTTTGCTTTAAGTCCTATTGCTATTCAATTAGCTGTTTATATATCAATAAAAATTTTTTTAATGATAACCTTATCATCAATTCTAACAGCTACGACTTCATCTATTGAATTGACAAATGGTTTGGAAGATTTGTTAAGTCCTTTTAAAATTTTACGATTGCCTGTTGCTGAGGCTTCAATGATGATTTCAATTGCATTACGTTTTATTCCGTCATTATTAGATGAATCAAAAAGAATATTAAATGCACAAGCTTCACGTGGTGTTGATTTTAATAATGGCGGACCTATTCAAAAATTAAAATCATTAATTTCACTAGTTGTACCATTATTTTCAATTGCTTTTAAAAAAGCAGAAGATTTAGCAAATGCTATGGAAGCACGTTCTTATAACCCTCGTTATGCACGAACACGTTATCGTACTTTTCCATTAAACCTAGCAGATTATGCTTTATTTGGAATTTTGTGTATTCTCGTTGGATTTTTAATATCATTGGCCGTGATTAAATTTTATTTCACTCCATTTGGTGTATTTGAAGCAAGTGCATTGTTTGCTAAGTAATTTGATGAAATAACTTGTTAAACAGGTTATTTTTTATTTATTGGAATAGGAATTAATAATTATGAATTATAAAATTAGTCTTCGTTATGATGGTAGTTTATTTTATGGATGAGCTAGACAACCACAAAAAAGAACGGTTCAAGGGGATTTAGAAGAAATTTTTAAATCAATTTTTAAAATTAATAATATCAGGATTATTGGTTCAGGACGTACTGATAAAGGTGTACACGCTTACGAGCAAACCTTTAGTGTTAAACATAGTGCGTTAAAATATGATTCGCACATTATTTATCAAGCACTTTGCTCACGAACTAGTTCTGATATTCAAATTTTAGAAGTGCAAAAGGTTGATGATTCATTTCATGCTCAATATAATGCTACTTCTAAAACATATCAATATGTCATTAATGATTATGAATTTGATTTGTTTAGAAATAATTATGAACTTTTTGTTAATCAAAAAATTAATGATCAAAAAATATTAGAAGCATTAGAATTGTTTGTTGGCGAACATGATTTTAAATCGTTTTCAACTAGTGAATTAACAATGACAATACGTCGCATTAATTGAGTTAAAATCAAAAGAGATACACACTTAATAATTTATATTAATGCAAATGGTTTTTTAAAAAACATGGTTCGTATGATCGTTGCTTCTTGTTTAGATTATGCTTTTAATAAAATAAGTTTAGCAAAAATTCATGAATTATTAATATTCCCCAAAAAGGGTGCTAGTATTAAATTAGCACCCGCTTGTGGATTATATTTGTATAAAGTCTATTATTAATGTTTTTTTTCAACATGTTTTTTTGGAATATTAGCTATCACTTGAATACCTAATGCTCCTTTTCAAACATATACTAAAAAGATTGGAGGACCAGATCTAATTACAATCTTTTCCTTATCAACATGAAAATGGTTAATTACATCATTTTTTAGTTCATCAATTCTTTCACTTGATAATCCCGCTTGTAGAATACATACTTTTTCAATTGTATGATTATTTAGTTGATTATCAAAAACATGGTTAATATTTTCAAGAATTTTATGGGGTGCAGAACGAATATTTAATGCCATTCCCGCACGGTGATTTTTTCATTCAGCTTTAATTATGGGTGTTACTTTACTTAATTTTAACATAGTACCAATGATTTTAGGGATTCGACCAGAACGAACAAGGCCAGTAATATTTTCACATGCTAAAAAACTACTTGTATAATCATGGTGTTTGTTAATCATTTTTAAAATATCACCAAAACTAATATTATTGCTTAACATTGCTTTAATTTTGTAAACTAGATGTTCTAAGGAATAGCCAAAGTCTTCTGTGTCTACAACAAATAATTTGTCTTGATATTCTTTCTCTAAATTTTTTGCATTATTGAATTGACTACTAAATCCACTAGAGATGCCAATAAAAATAATTTTTTCATATTCTTGTAGTTTTTGTTCAATAGCTTCAATTAAAACACCATATGGTGTACAACTTGTTTGAAAGGTGTATCCATTATCAATGTCTTCATAAATTCGCTCTGGCTTTGATTCTATACCATCATCTACAATTAAAGTATGATCACTTCGTAAAATAGAAAGCGGCAGAATCATAACATCATTATTTTTAGCTCATTCACGATCTAAAGTCGTTGATGAATCTGTCATGATTAAAAATGATTTTTTCATAACCTTGTCCTTACTTAATTATTTTAATATAGGTTTAAAACAAATTATATTTTAGCAAAATATGGATTTTATAGAATTATTATATTAATATAACGTTATATAATATACTTAGTAAATAAAAATAATATCAAAAATATTTTATCGAGGTATTTATGTATAAGTTTGATCAAAAACTAAATCACAGTGCTGCACATCTTTTAGCAATGGCACTAACAAAATTTTATCCTAACTTAAGCCTTGCGATTGGGCCAACGATTGATGAGGGGTTTTATTATGATTTTAATCTAAATGATCCAAATACCTCAATTACCCCTTTAGATTTATTAAAAATTGAAAAAGAAATGAAGAAAATTACTACTCAAGCTTTAACGTTTGATTATGAACAAGTTACTTATGAAAAAGCAAAGGAATTGTTTAAACACAATAAATATAAATTAGATATTATTGAACAAAATAAAAATAATTCACTTTCAATTTATCACTCAGGTAAATGGTTTGACTTATGTAAAGGCCCGCATGTACAAAACACAAAAGAGATCAAGGCAATTAAATTATTAAATATTGCAGGTTCATATTGACGAGGTGATGCAAATAATGATCAATTAATTCGTATTTATGGGGTTGCTTTTAGTGATCAAGATCAATTAGACGCTTATTTAAAAGATTTACAAGAACGAAAAGAACGTGATCATCGTAAAATTGGTAAAGATTTAAACTTATTTACTTTTAATAATCTAGCAGGCCAAGGATTACCAATTTGATTACCTAATGGAACCATTATTAAAAATCAGGTACAAAAGTTTATTAATGAAGTTGAATTTCAATTCAATTTTGATACTGTTATTACGCCTATATTAGGTAGTATAGATTTATATAAAACGAGTGGACACTGAGATCATTATAAAGATAATATTTTTTCACCAGTACAAATTGATAATGAAATCTTAGTACTTCGTCCAATGACATGTCCTCATCATACATTAGTTTACTCTAATGAACTTCGTTCATATCGAAGTTTACCAATTCGTTTAAGTGAACATTCAATTTTACATCGTTATGAATCATCAGGTGGATTAACAGGCTTTGAGCGGGTTCGTGAGATGATTTTAGAAGATTGTCATGTTTTTTGTCGCTTTGATCAAATTGAACATGAAGTTATTAATGCTTTTAAAATGATTCAAGAAGCTCAAGAAGGATTGGGTATTAAAACATTTGAAATTCATTTATCTTTAAATGATCCTAATGATAAAAAAAAATATTATGATGATCCTCAAATGTGAGAACAATCACAAAATGTGTTACGTAAAATGTTAAAAGATCACAATATTCCTTATAAGGAGATGGTAGGAGAAGCGGCATTTTATGGTCCAAAAATTGATTTTCAGGTTAAAACTGTTTTAAATCGAATTATTACAGTTTCAACTATCCAATTAGATTTTTTATTACCAAACCGTTTTAATTTAACTTATATTAATGAAAGTAATGAACAAAGCGTACCGGTAATGATTCATATTGGTATTATTGGAACTTATGAACGTTTATTAGCAATTTTATTAGAACAAACTAAAGGTATTTTACCGCTTTGATTATCACCTGTTCAAGTAGTTATCATTCCTGTTAATGAAAATTTACATACTGATTATGTAAAGGAACTAAATATTAAGTTAAGAAAGCATTTAATTCGTAGTAGTGTGGATTTACGTAATGAGCGTTTATCAAAAAAAATTCGTGAAGCTCAAATTCAAAAAATCCCTTATCAAATTGTTATTGGTGATGAAGAAATTAAAAACAACAAAATGGTTACTTACCGCTGCTATGGTAGTGAAAAAACGACAACAGTTTCTATTACAGATTTTATAAATATGTTAGAAAACAAGATTAGATTAAAAAAATAAGTTAAATAGCTTTATTTTTAATATAATTAATAGATTATTGTTCGAAGAGGTGACTACATAATGGCGATTAAACGAGGCGTTAGATTACAATGTAATGAGAGTAAAAGTATTAATTACATTACTACAAAAAATGCAAAAAATAATCCAGACAGACTTTCTTTAAATAAATTTTGTCCTAAATGTCGAAAAGTAACAACTCACGTTGAAATTAAGAAAAAATAGGTATTAAATTATGAGTGATTTCAAATTACAACAAGTTTTAAAAACAATTAAAGAACATGAAACACAAGGCGCTGACGGTATGATTTTATTTTCTAATTATAACCGTTATTGATTTTTAGAATTTGCTTCAAGTGATGGTTTTGTGTTTATTAATAAAGATGGTAAAGCAATTTATCTAGTGGATGCTCGTTATTATACAGCAGCGTCTGAAACTGTAAAAAACGCAAAGGTAATTTTATTAGCTCGCACACCACAAAAATCAACTTTTGATCTACTAAAAGACGCAATGGTTGAATTAAATATTACTAATGCATTAGTTGAAGCTGATTATGTAACATTAGATATTCATGAAATGTTAAAAAAATTAGTAAAAAATACTACTCCTTTTACATCAGCTACATTACGTGCTATTAAAACTGAAAAAGAACTTGAATATTTACAAAAAGCTGCTGATATTGCAGCTTTAACATGTAATTGAATTCGAGAACAAGATATTATTGGTCGAACAGAACTTGAAATTGCAACATTAGTTTCAAAACATATGTTAGAATTAGGTGGAGAATTAAATTCATTTGATCCAATTATTGCTTCAGGTCCAAATGGCGGATCACCACATCATCATCCAGGAAATCGTGTAATTGAAGATGGAGATATGGTAACTGTTGACATTGGTTGTACTTATAAAGGTTATTGTTCTGATATTACAAGATCTTTTATTGTTGGAAATAAAGCAAATCCACAAATGCAAGAAATTTATGATAAAGTTCTTGAATCTCAAACAGCAGGGATTAATCTATTGAGTACAAAAGTAACAGGTCAAGAAGTTGATAAAGTATGTCGCGACATTGTTGATAATTCTAAATTTAAGGGCTATTTCACACATGGAACAGGTCATGGTGTAGGTTTACAAGTACATGAATTACCAAATACTAATGCTGGTAATCCTAATAAATTGCCATTAAATGCAGTTGTTACTGTTGAACCAGGAATTTATATCCCAAATGTTGGTGGTGTAAGAATTGAAGATACAATTGTAGTAAAAGACGGACAAGCCCTGGTTTTAACAAGGTTAGCATATAAATAAACTACAAAAAAAGGGTATAGTTCAATTGGTAGAACAGCAGACTCCAAATCTGCGTGTTGCGGGTTCGAGTCCTGTTACCCTTGCCATTTAAAATAAAAACCATCAATAATGATGGTTTTTTTACTCTTATCATTTATTAAAAGGTTATAACTTTTTTCAAAATGTTATAATATAGTGTTAGATATTTGTAACATTTGGAGTTATTGAAGTATGAAATATAATGATATTTATATGGGATTATTAGAGTTATTAAAAAAAAGTTATAGTCCTTATTCAAATTATCCAGTTGCTGCTTATGTTGATACTGATTTAGGATTTATCTCTGGTGTGAATGTTGAAAATGGATCATTTGGATTAACTTCTTGTGCTGAAAGAAATGCAATCTTTAATGCAATTGCAAATGGTGCAAAAACATTTAAAACAGTTTATATAATTACAAAAAATAATGGTGATATTGCGAGTCCTTGTGGTGCATGTCGTCAAGTGATTTTTGAATTTTTTAAAAAGGATGCAAAAATCGTCGTTTTTAACAATGATGGTACTTATAAAGAATATAATGTTGAACAATTATTACCATTTGGATGAGATCCAAATATATCTCTATAGTTATTCATTATAAAATAAGAAAGGAGATAAAATTATGGAAAATACAAATAAAATTTTTAATTGTTGATTAAATTCACCAAAAATTCCTCAAGTAATTAAAGAAGAATATAAGAATAAAACTCAAAAAGAAATTGATCATATTTTTGATGAAAATTATCGATTTAAATTCGGTACATCAGGTGTACGTTGCATTTATGAAGAAGGAACTCGTTATTTAAATGCAATAACTTATACTCAATTGACATTAGGTTTTATTAAATATTTAGAAACGAAAAAGAATTTTAATAAAATTATTGTTATTGGCCGAGACAACCGCTTTGGCTCACGTGAAAATTTGAAACTAGTTGCTGAAATTTTTAGTAGTTTTGATTATACTGTTTATATTAATGAAGATTATGGGATGTTATCAACTCCAATTACTTCATTTTTAATTAATCAATTACAAGCAGGAGCTGGAATTATGATCACCGCTTCTCATAATCCTAAAAATTATAATGGTTTTAAAGTTTATAATGCTAATGGTGCTCAGCCATTAGTTGATGATACTAATCTCATTGAATCTTTAATGCCATCATATATAGAAGCATTGGATTTTAACTTTGAATTTAAACAAGATAATATTAGATTTTTAACAAAAGAACAAATTCAAGGTTATTTTGATGCAGTTAAAGCACAACTAATTAATACTGATCCTAATATTAAAAAACCCTTCAAAATTGTTTTTAGTGGTCATCATGGAACAACAACAAAGGACATGATTCCTTTTTTAGAATCACTTGGTTATGATATGGTTAGCGTTAGCGAACAAAATTTTGAAGATCCTAATTTTAATGATGACCCAAGTTCTAATCCAGAAGAACAATGTTCATTTGATTTAAGTGTTGAATATGCAGACAATACAAACGCAGAAATAATGATTGCATCTGATCCTGATGGAGATCGAATGGCCATTGCTGTACGTCATGAAAATTATTGAAAATTTTTGACTGGTAATCAAACAGGTGTTTTAATTGCACATTATTTATTAGAACATAAAAAATTTACTAAACCTACATATATTATTTCAACATTTATTTCAACACGCTATCCAGAATTATTTGCTAAAAATTTTGATTGTGATGTTTTATATGTAGATGTAGGTTTTAAAAATCATGGTAATTTAATTGCCAATCGTAAAAAAACACATGATTTGGTTGTTGGTTTTGAAGAAGCAATTGGTTCATTACCATCTGATATTAATAATGATAAAGATTCATATCAAACTGCGTCTATTTTGTTAGAAATGATTAATTATTATTATGAACAAAAACTTGATTTATTTACCGTTTTAAAAAAAGAGATTTTTGCTAAGTATGGGAATTGATATAGTAAAACCGCTCAGTTTGTTATTGATGGTGATAACTGAAAACAAAAAGCACTTATTATTTTAAATAACTTAAGAAATTTTGAATTTAAAACAGTAACCCAATACACCATTCATAACATTATTTTTCATGAACAAGGATCTTATCTTGAATGAAAATTAGACGAATACTCAACAATCAAATTTAGATTATCTGGAACAGAACCTAAATTTAAAGTATATATTGATTTATATGATTTTAGCAAAGATCAAAAACGAGATTTTTATCAAGAACTAAAAATTAAAGCTGAAGATATTTTAGATTTTTAAAAAATTATTTAGGTCTTTAAAATCACAAAAGATGAATATTAAAAAATGGCTTGATAAAGCGAGTCATTTTTTTTTTTTTTTTTGATATTTTTCATAATTAAAGATTGTGCTAATAGTATTTTCATAAGTCTTAAGTTATTTGTTAGTTAATGATGCTAACTAGTTTATTATTATTTGTTACTGTGTATTTTTATAATATAATCTTAATAATTAACTTTTTGAAAATAAGATTATAAATCATGAAGAAAAAAACAAAAATATTAGTTTCAATATTAACAATCTTTTTAGCTAGTTTAAGTATGGTTGTCCTAACAACATGTTCAATGAATAAAAAAAACTATAAGAAATTTGATGAATCTAAGCAAAAATTACTTACTTTAATTAATGATTTAGATTTAGAAACTCAGAATGATATTAAAAAATGATTAATAGAACAAGAAAAAAAGTTTAATACTAATTATTCAGATACAGAAATGATCATTAAGGATATAGAACAATATACTAAAGAAGTTTATGAGAAATTAGAACATTTAAAAGAAATTAAAAAAGAAAATCTAACTGAATTTAAGAAAAATAAAGATGCATTAAAAAAAATACTTCAAAACTCTGATGCTAAAGAAGTTGATTCTAATGAAATTAATAGGGCATTAAATAACATTCAAATAACAGATTTATCAACAATTATAGAAATAAAATCAGCACTTAAACGAGTTAATAATTTAAAAAAATATTAGAGCAAAAAATTAATGAATTAAAAATTAATGAAAAACAAAAATTTAATATTAAAAAATTAGAATTAAAAAAATTACTTAATTCAAATGAAATTGCTTCTATAGACCACACGTATGAAACAAATTTGTTTAATAATAGTGAAATTAATAATCATTCAACGATTAAAGACATTAGAACTCAAAGAAAACAAATTGAAGATGCAATTATTAGTTTAAATCATAAAATTAAGATTTTTAAAGTGTTACAAAATAAAGAAAAAAAAGCTAAATTTGAAGAATTTAAAAGTATAAAATTGCAAATAGATAAAATTAAACGAACACAATTTAATGGCAATAGATATATAGAAATAATAAAAGAAAAAAGCGAAAAATATACGTATATTAGCCAAGATTCTGATCTAGTTGATATTCAAAATGCAATTAAGGAATTAAATAGAATAATTCAAATAAATAAAATAAAAATAAGTATTGATAATTCAACTAAATTAGACGAAATAAAAAAACAAGAATTTATAAAAGAGTGTGAGAATTTACTTGATATTAATAATTACTCAAATACATCATTAAATAATCTAAAAATAAAGATTAAAACAGCTGAAAATACAAATAATAATACAGTCAATAAAGATTTAGATAACCTTCAAAAAGAGTATGAAATAGCAAAAAATAATGTTAAAACTAAATTAATTATTCTTAAAAATAAAACTGATGCTGATTCTAAGCATGTTTTAGTTAATTCCAATAAAGTAATTAATAGTAAAATAGAAAGTATAATTAGTCAAATTGATTCTGATTATCATAAAACATACAATAATCAAAAAAAGAATATAGATGAGTGCAAAAAATATATTGATCAACTTAATAATTTGTATAAAATTAGTGCTTTATACATTGATTATTTTAGTGAAGTTAATGAAAATATTAACGCTAAAATTGATGATCAAATTATTAAAGATCAAATTTATAATTCACAAGTAATTAATCCTACAGAAGAAGTACGTAGCAAACTAGAATATTTAAATTGATCATATTATACTATTAATTCACAAACTGATCAATTTATTCAAACTTATAAACAAACTTTAAATTCTTCTTTACGTCAGATAAAATTAAAACTTTTTAATACAGTTATGGATAAAGTATTTCAAACTGCACGAGAACCACATTCTAATAAATATAATTTAACAGCTGTTTTAACAAAATTGAATGGTACAGAAAGTGACTTAATTTTTAAAAACCAATTAACAATTTTTAAGGGTTATGAAAAACTAATTATTAAATTTAAAAAACAAAACGAATATAATGAGACTGCAAAAGGAATTTATCAAGAAAACGGAAAATGACATTTTGAAGGTTGAATAGTGATTTGAAATAGTAAAGATAATATATACTATTATTTAGGTCATTCATATCCAATTGTGATTAATATGGTTGAAAAGCCATCAAATTAAACCATCTTAATTAAGAAAAATAAAAATTTCCTTTATTTAAATTAAGGGAGATTTTTATTTTTTTATTATTGATACTAATTTAGTTGTATGATGTTTGTATTTAATATCATTTATTTAGTAAACCATAAAGCCACACTTGACTTGTATATACAATTCATAATTATACAATAAGATTCATGATTTAATCTAATTAATTTTTTAAAAAAATAAATAAATTTCTATTATTAATATTTTTAATTTTTCATTACTTTATAATTGGTTTTTCATACTATAATTTTGGTATTAAAAATTTAAAAAAGATGTTTTTTGTTTAAGGAGTTTTTATGAAATTATTGAAAAATAAGAAATTTTGAGCTATATCATTAGGTTCAATAATAGTTAGTGCAAGTATTATTGCTACAGCTACTGCTTGTACTAACTCTAATATCGAATCAACTGTAAGTTCATCTTTTGTTAGAACTCAAAATGGTGTTTATGCAATTTATGAGATTACAAATTGAACTAAATTAGATGATAGCCAAAAAAAATCATTACAAAATCTTAATTTTAAAACTAGTGTTATTGATAAAGATAATAATGCTGAATTTACATCATCTACAGGGATTTTAAGGGATGGTAGAGTTTATGTAAAATTAGCACGTGAACCAAAAAAAGATGATCGTGTAATTATTAAACCAGATGATGCTAATTTAAAAAAAGGTGCTGTTTATGTAACAACACTAAATATAAGCAATATTGAATTAAATGATGGACCAAAGGTTCAAATAAGTCATAGTAATGAAAAAAATGAAATAAAATCATATAATAGTGCAGAATCAATTATTTACAACCAACGTTGATTAGCTAATATTTGAAACACATTAAGTGCTGAAAAAGATGGGATGCTATTAACAGAGTATAATAGTGCAAAACACCAATTTGATGCAATGATTAAACAAAGTGGTTTTGATACAAAAAAAGTAGAAGTAAAAAAAGATTCAAATGGTAATATCACAAAGGTAACAGTTTCTAATCCTGAAAGTAATAAGGCAATTCCTGTAGTATTTATGGATATTGATGAAACAATTTTAAATAATTACGCTAATCAAAACTATCAATTATTAAATAATAAAGCTTATAATCCAAAGGATTGAGATGCATTTGTGAATGACAAGGTTTCAAAACGATTAGCTGGAGCTTTTGAATTCATTAAATATGTTTGAGAACATGGTGGAGTTGTAATGTTTAACTCAAACCGTGAACAAGAAAACCAAGTCGATCCAACTATTGAAAACTTAGTTAGTGAAGGTTTAGAACGTAGTTTATTACCTAAATGAATTTTTTGAATGCAAGGAGTAGATTTTAGTAGTGATAAACCATGAGCAAATATTAAAAAAGATGCACAAGGAAAACGTGTAAAATCTACTAAAGAAGATCGTATGAATGCCATGAATGAGCATAAAAAAGGTTATGATTTAAGTGAATTCGGTAGTGGTAATGCTGTAGTTTTAAAAACTGTAATGCGTATTGGTGATAATTTTGATGACTTTAATGATAATGCTTCAAAAGGTAAACTTAATAGTGAACGTGTAGCATTATTAAAAGAATATGGTAAATTATTTGGTAATTTTGATATAAATAATAAAGGTGTTAAATACGAAAAAAATCAAAACGGTCAAATGGTGAAATCTGATGAAACTTGATCAGAATCTTATGTATTAATAGGTGGTAATTCATCTTATGGTGGTTTTGAAGCAGGAATCGCTAAAGGCTATTATGGATTAGGCAAAGAAGGTCAAGCTAAAGCACTAAAAGATTACATAAAACAACTATATTGAGAACCAAAAAGCAAATAAATAATTTTTAATTTCATATTTAAATCCTAAAAAAAGAGTAAAAAATTTGTTTTTGCTCTTTTTTATTTACTTTTATAATTTTTTCGTTTATAATTTGTAACGTGTTTTATCAATCGTAATTTTACTTTTTTGATACGCACGGAGTCGTTGTCTATAATTAACATAAAAAATGAAAGGAAATTTACATGCCTACAATTGCGCAATTGATTCGTAATAAACGAGCACCTAAAGTAAAAAAAACTAAATCACCTGCTCTTTTATTTACATACAACTCATTACATAAAAAAACAACAAAAAACCCATCACCTCTAAAATCTGGTGTTTGTACTAGAGTTGGTACAATGACACCTAAAAAACCTAACTCAGCTTTACGTAAATATGCTAAAGTTCGTTTAAGTAATGGTTTTGAAGTTTTAGCTTACATTCCAGGTGAAGGACATAACTTACAAGAACACAGTGTCGTAGTTATTCGTGGGGGAAGAGTTAAAGACTTACCAGGGGTTCGTTATCATATTGTTCGTGGTGCTGGCGACGCATCAGGTGTTGAAAAACGTCGTCAACAACGTTCACTATACGGTGCTAAACGTCCTAAAAAAGAAGCATCTAAATAATTAAATTTAGTTAGGAGATCGAAAAAAATGAGAAAATTAAAACCACAAAAAAGACAAGTTTTAGCTGATCCAGTATATAACTCTCGTCTTGTAACTAAACTAATCAATGCTATTATGTATGATGGTAAAAAAGGATTAGCACAATCTATTATTTATTCAGCCTTTGAGATTGTTGAACAAAAGACTGGCAAACCAGCTTTAGAAGTTTTTAATAAGGCGATTGATAATGTAATGCCAATTATTGAACTTAAAGTAAGACGTGTTGGCGGTTCAAACTTTCAAGTACCAACTGAAGTAACGCCAGAACGTCGTCAGACTTTAGGTTTAAGATGAATTACATTATATGCACGTTTAAGACACGAACATACAATGATTGAGAAATTAGCACATGAAATTATTGATGCTTCAAATAATGTAGGAGCAGCAATTAAGAAAAAAGAAGATACTCATAAAATGGCAGAAGCAAATAAAGCTTTTGCACACCTACGTTGATAAAATTTGTTTTAAGGAATTGTTAATATATGTCTAAAGAATTAAAATTATTTCGTAACTTTGGAATCATGGCACACATTGATGCTGGTAAAACAACAACCTCTGAACGGATTTTATATCATACAGGAAAGAATCATAAAATCGGAGAGACTCACGATGGTGCTGCAACAATGGATTGAATGGCACAAGAAAAAGAACGTGGAATTACCATTACATCAGCAGCAACTTATGCAAAATGAAAAGGTCATAGTTTAAATTTAATTGATACACCGGGACACGTTGATTTTACTGTTGAAGTAGAACGTTCTTTGCGTGTTTTAGATGGAGCTGTCGCTGTTCTAGATGGTCAAAATGGAGTTGAACCACAAACTGAAACAGTCTGAAGACAAGCTACTAAATATAATGTTCCAAGAATCGTTTTTGTTAATAAGATGGATAAAACAGGTGCTGATTTTTATTACTCAATTGAGACAATGAAGAATCGTTTAGGTGTTAAAGCCACAGCTATTCAAATTCCAATCGGTGCTGAAGCCGATTTTGTTGGAAGTATTGATTTAATTGAAATGAAAGCTTATATTTATGATGGAAAAGCTGATGAAGAATATAAAATTGAAGATATTCCAGCTGATTACTTAACAAAAGCACAAGTAATGCGTTCACAAATGATCGATGATGTAGCTGTTTTTGATGATGAAGTGATGGAAAAATACTTATCTGGTGAAGAACTAAGTCATGAAGATATTAAAAAATGTATTCGTAAAGGAGTAATTTCAACTGAATTATACCCAGTATTATGTGGTACAGCTTTTAAAAACAAAGGTGTTAAAAAATTATTAGACGCAGTTGTTGATTTCTTACCTTCACCAATTGATGTTCCACCAATTAAAGGTGTTGATGATCATGGTAATCCAATTGAATATCATAATGATCCAAATGAACCATTTGCTGCCCTTGCCTTCAAAGTCGCAACTGATCCTTTTGTTGGACGTTTAACATATATTCGTGTATATAGTGGTAAACTTGACAAAGGTACTTATATTTACAATGCAACAAAAGATAAAAAAGAAAGAATTTCTCGTCTTGTAAAAATGCATTCAAACAACCGTGATGAGATTGATTCAATTAGTGCTGGTGATATTTGTGCTGTAATTGGATTAAAAGACACTACTACTGGTGATACAATTTGTGATGAAAAAAAACCAGTTATTTTAGAACAAATGGTATTTGCAGAACCTGTTATTTCTTTATCTGTTGAACCAAAAACTAAAGCAGATCAAGAAAAGATGAGTTTAGCTTTATCTAAATTAGCTGAAGAAGATCCAACTTTTAGAACATACACAAATGAAGAAACAGGTCAAACTATTATTGCTGGAATGGGTGAATTACATTTAGATGTTTTGGTTGATCGTATGCGTCGTGAATTTAATGTTCAAGTTAATGTAGGTGCTCCTCAAGTTAGTTATCGTGAGACATTTACTGAAGTTGCTGATGCTGAAGGTAAGTATATTAAGCAATCTGGTGGACGTGGTCAATATGGGCATGTCTGAATTAAATTTGAACCTAATCATGATAAAGGATTTGAATTTGTTGACAACATTGTAGGTGGTAAAGTACCAAAAGAATATATTAAAGAAGTTGAAAACGGCTTGATTGAGGCATTAACATCAGGGCCAATTGCTGGTTATCAAACAATTGACGTTAAAGCAACTATTTTTGATGGTTCATATCATGATGTTGATTCATCTGGTATGGCTTATAAAATTGCTGCATCTCTTGCTTTTAAAGAAGCAGCAAAAGTATGCAAACCAGTTTTATTAGAACCTATTATGTCAGTTGATGTAACTACACCTGATGATTATTTTGGGACGGTAATGGGCGATATTTCAAAACGTCGTGGTGTAATTGAAGGACAAGAACAACGTGGTAATGCACAAGCTATTAAAGCTAAAGTTCCGCTATCAGAAATGTTTGGATATGCAACGGATTTACGTTCAAATACACAAGGACGTGGTCAATATATTATGCAATTCAGTCACTATGCACAAGCACCTAAGTCTGTTACTGAAGAAGTAATGGTAGCACGTGCAAAAAAATAATTATTTTAACAATTAACAATAAAGTATTTAATTAATAATCTAAAAAGTTAAAATGTTATAATTATAAGGTATAATACCTTTTATTATGTATTCAAAAATTATTTAGAAAATAAATTAATTTTTAAGGAGATTTAAAATGGCTAAAGCTAAATTTGAAAGAACAAAACCTCACGTTAACATTGGTACTATAGGCCACGTTGACCATGGTAAAACTACTTTAACAGCTGCAATTAGTACTGTACTTGCCAAAAAAGGACAAGCTATTGCACAATCATATGCTGATGTTGATAAAACACCAGAAGAAAGAGAACGTGGTATTACAATTAACGCTTCTCACGTTGAATATGAAACAAAAACACGTCACTATGCACACGTTGACTGTCCAGGACATGCCGATTATGTAAAAAATATGATTACAGGGGCAGCACAAATGGATGGAGCAATTTTAGTTATTGCTGCATCTGATGGGGTTATGGCTCAAACTAAAGAACATATTTTATTAGCACGTCAAGTTGGTGTTCCAAAAATCGTTGTTTTCTTAAACAAATGTGATTTCATGACAGATCCAGATATGCAAGATCTTGTTGAAATGGAAGTTCGTGAATTATTATCTAAATATGGATTTGATGGCGATAACACACCAGTTATTCGTGGTTCAGGTCTTAAGGCTTTAGAAGGAGATCCAGTTTGAGAAGCAAAAATTGATGAATTAATGGACGCAGTTGATTCATGAATTCCATTACCAGAACGTAGTACTGACAAACCATTCTTATTAGCAATTGAAGATGTATTCACAATTTCAGGACGTGGTACAGTAGTAACTGGACGTGTTGAACGTGGTGTATTAAAAGTTAATGATGAGGTTGAAATTGTTGGTCTAAAAGACACTCAAAAAACTGTTGTTACAGGAATTGAAATGTTTAGAAAATCATTAGATCAAGCTGAAGCTGGTGATAATGCTGGTATTTTATTACGTGGTATTAAAAAAGAAGATGTTGAACGTGGTCAAGTACTTGTAAAACCAGGATCAATTAAACCTCACCGTACTTTTACTGCTAAAGTTTATATTCTTAAAAAAGAAGAAGGTGGACGTCATACACCTATTGTTTCAGGATACCGTCCACAATTCTATTTTAGAACAACAGATGTAACAGGTGCTATTTCATTACCTGCTGGTGTTGATTTGGTTATGCCAGGTGATGACGTTGAAATGACTGTAGAATTAATTGCTCCAGTTGCGATTGAAGATGGATCTAAATTCTCAATCCGTGAAGGTGGTAAAACTGTAGGTCATGGTAGCGTAATTAAAACAAGTAATTAATAATTTTAAAAAACAAAAAACAAAAAATGACTAAGTCTTTTAGTAAACTTGGTCATTTTTTTCTATAACTCTATAAAAGGAAATTTAGTTTAAATGAATGATATTAATGATAAAAAATATGATTGAGATATAGATTCATTATTAAAAGGCAAGAAACTAGATATTTTGTTCAATGAATTTGTTGAATCAAATAATGAATTAATAAAAGCTTTTGAAAAGTGGATTAATAGTTTAAATGATTTTATTAATTTTCACGAAGTTTCAGAAAAAAATTTATTAATTAGTAATCGAATTAACAATTATGTTTCAAATAAATTGCAAACAAATTTAATCGATAATGAAATGCTTGCTTGGTCTCAAAAAATTGAACATGAACAACATCGTGTAGCTAAAATTTTTATTAATTTTGAAAATTTAGCAATAAAAAATAAAGATTTAATTAATTCATATTTAAAAAATTCTTCATTAATTAAATACCAATTAGAATATGAAGAATTATGACGTTACGAAAAACATATTTTAAATGAACAACAACAAAAAGTTGTTACAGCTATTAGCCGTTTTAGTTCAAGTTTTGGTGATATTTTTGATGTTTTATTAGATTCAGATATGCAGTATCAGGATGGTATAAACTATAAAAAACAAAAAGTTTGTTTTAAAAATCAAACTGATCTTTATGTTGCATCTAAATCAAATGATCGTGCTTTACGAAAATCAGCTTATGAATCTCATTTTAAAGCGATATATGATTTACGTAATACTTTTAGTAAATTATTATATTATGAATATGTTAAACAAAACGAGTTAGCAAAATTACATAATTTTAAGGATTATATTTCTGCGGATGCTTTTAGTGATAAGGTTGATAAAAATTTTATAAATCATATTTATACACAAACAAAAAAATTTGCTAAAGGAATTAATAGATATACAAAATATCGAACATTATTTCTAAAACAACAATATCAATTAACTAAAGTTGAACCATGAGATAAAAATTTAGATATAATTGATAAAAAAAACATGTTTAGTATTGAATCTGCTAAAAACTTAACTTTAGAAGCTTTGGCATTATTAGGTAGTGAATATATAAATGTTGTACAAAGAGCGTTCAATGAGCAATGAATATCATGAATGCCAAATAATAATAAAATTTCTGGAGCATATTCTATAAGTAACACTAAAGGCTTAGATAAAATTTTTATTTTAATGAATTATGATGAAACTTATAATTCTATTTTAACATTAGTTCATGAATTAGGGCATTCTGTACACACTTATTTTGCTAATCAATCACAAGAAGTATATAATGAATATGAAACCTTTTATGCAGAAATTGCTTCCATTACTAATGAGATCTTAATGAATTACCATTTATTAAAGAAATACGAAAATGATGATTTAATGCGTCTTTATATTTTAGATGAAATGATTAGTGGTTTTATTGCTACAACTACACGCCAAGCGATTTTTTCAAATTTTGAGTGAGTTGCCAATGAATGAATTAATCAAGGTGAAGAATTTAGCTGAAATAAAATAGTTTTAGCTTATCTAGAAATAAATCATGATTATACAGGATATAAATATAATAAAAACAAAATCAGTAAATATGATGAAGCAAACGCTTTAATTTTAATTAACATTCCACATTTTTATACTGGTAATTTTTATGTATATAAATATGTTATTGGACAAATTTGTGGATTAATTAATGCGATAAGAATTTTCAATAATAAAGCAAACGCTAAAGAAAAATATTTCTGTTTTTTTAAATCTGGAGGGAGTTTGTCTCCTCTTGAAACAATTAATATTTTAGATATAAAAATTAATGAGAACGATGTTTGAGAAGAAGTTAATATAATTTTTAATAGTTGAATCGATGATTATATAAAAATAATTAAAAAATTTTTAATAAATAAAAAAATATAATTTTTAATATATTTTATGATATAATCTATATGGTGTTTTTATATAAAAGGGGGAGATTTAATTTGGAAGAACAGAAACAAATGAAAGTTATTAAAAAATCCGATTCTTATTTTAAAAAAAATCTTACCGTTTTTAGAACACTTTTAGAACTTTTAAAAGAAAGCTCGCCATTTATCGCTGCAAGTGTTTTTACAGGACTATCATTAATTTTAATTGTTGTAATTGTTGCTCATCATAAAGATATTGGTGCTTATGCAGCAACTAGTATTAGTTATTTAACTATCTTTCAGTTAAGTTTTTTACAATTAGGAACAACCTTTGGCATTGTTTTTGCGATTTGGTCAAAAAGATCATTTAAAGATGGTAAGTATAAGTTTGAACCTACAAATGATACAGTAAATGCTGCTAGTTTTTATTCATTTATCTTTGGTTTAGTAGTTTTATTAATTTATTTTACAACCTCTTACATTTATAATCATTTTGCAAATGATCACCAAAATACAGTGATTAATCAAAGTGTAGGTAGTGCATATATTGTTTCAGGATTAGCAGTTGTTGGTTTAGCACCTTTGCGAAACTATTTATTAATGTTAATAAGATCAACCAAGTCCATTAATGTTTTATTAGCAATTGTTTTAGATTTTTGTACATGAACAACCGCATTAATTGCAGCTTTTTTATTAGGTAGTTATAGTGTTTTAAATTATTGGGGCTATGGCCTTGGTATGTCAATTGGTTTTGTTTTTTGAACTATTATTATTGGTATGACTACAATTGAAAAAGCCCAAATTGTTTTACGTCCATTATATATTTCAAAAAGATTATTTGTTTTAAGTATTAAATTATTATGAATTCAAACGATTTTATCATCATTAAAATCAGTTGGTAAAATGTTTATTTTGTTAATTACATTTGTGGTAATTAACTCACGTGTAGTTGGTTCATCATTATTAGATTTTCAATCAACGAGAATTCTAATGTACCAATCAATGATTTTCATACAAATGTTAAATTTAGGATTGTCAGATTTTTTATTTTATTTATATCAAAAACAAGAAGTCAGAGACTGTCGTTATCATTCAAGACAATTATTTTTTTGAGTTTTTATATTAGGTATTTTATTTGCTTTAATTACTTCAGTTATTTTTGGTTTTTCTGTAAAACAATTAGTCCAATTGTATACAAGTGAACAAAGTCCATCTTATATATTTTTAGAAAAGCAAGTTCCCGAACATTTCTATCAATCTTTAAGAAAAATTTTAATTAATGATGAAAAATTATTAGATATTATTACTAGTCATGTGAATATTGGTCGCCAAGAAGTTGTTAATGCTTTATCATCAAATGATAAAAATATTTGATTACCAATGGCGCAAAAAGTGATTGATCCAATATGAAAACTAGGTGATAAATTTGAAACGATATATCAAATGAAAAATCCTTTTACACATCGATTTGTTGATATTTCAGCTTCAAATATTTATAGTTATTTAATTAAAAATAATGCTTATATTTTATTATCATTCTTTTGTACATTCTTTTCTTTTTCTTCTATTTTAAGTCGTTATGTAGGATTAATTGCCCGACGACAACGTCCTCCATATGTAATGATTGTTATTCAATTAGTTATGGTTGCGTTTACTTCTGGATTTGGGTTAACACACCAATCAGATCCACATTTCATTGGTTTAATGGCTTGATCATTTCCTTTATTTATATCAAGTATTTTTATTTTAGGATATAGCACTTATAAAATTTTACGTATTTATCGTAAATATTTAAAGAATCATAAGTACAATGATGAAAAATTATCAATTATGCCTAAAAAACAATTAGAACAACAAATTAAGGTTCGTCAAAATTAATATTATTTAATATCCACATATTAAATAATATTTTTATATTAATGGTATAATTTTTAAATGTATTACTTATTTTGAAAAGGAGAAAATTATGACATCATCGATTGAAAAAACGCCCCTTGTAATTAAACGTCATGTTTTTAAAAAACATTATGGCATTTTTACAACATTATGAGAAGTCATAAAAGATAGCTCATCGTTTATAGCTTCAAGTTTGTTCTCATCGTTAGCTTTATTATTAATTACAATAATAATAGCGTTACATAATTTTAGTGGTGCATTTGCTGCGACAAGTGTTAGCTATATTGTTGTTTTCCAATTTAGTTTTATACAATTAGGTGGAAATATAGGTGTAGTTTTAGCCTTATGAGCTAAAAAACTATATGATGGAAGTAAACACAAGTTTGTTCCATCACATTCAACAACTAACTTAGCAAGTTTTTATGCATTTTCATTCGGTTTAATAATGTTGGGTGTTTATTTGGCGACATCTTATACTTATAATCTTTATGCAAACATTCATGAAAATACCTTGTTTGCTCAACTATTTGGTGAACAATACATATGATCAAGTTTGGGGATTATCGTTTTAGCACCAGTTCATAATTATTTTTTAATTTCTATTTGAACAAATGATCGTCGTAAAATTCTATTAACTATTATTTTAGATTTTGCCACATGAACAATTTGTTTAGTGACAAGTTTTTTATTAGGTAAATATAGTATTTTAGCTTATAGTGGGTATGGTTTAGGATTGTCAATTGGTTATATTATTTCAACAATTGCCATTAGTATTATTAAATTTAGTAAAGATTGAAGGATATCTAAATTAGGTTTATCATTAAATTTATTAAAAATTAGCATTAAACAAATTTGAAATCAAACTTTAATTTCGATTTTTAGTTCAATTATAAAAATGTTTGTTCTATTAGCTTTATATCATTTAATTAATCAAAAAATGACAAATTCTGTTCCTTTAAATCTACAAACTGGTCGAATTTTATGATATCATGCGATGTTGTTTATTCAAGGGATTGGATTAGGTTTTACTGATTACCTATTTTATATTTTTCAAAAACAAACTATTCGTGATCGTCGTTACCATTCGCGCCAATTATTTATTTGTGTTTTTGGTTTATTGTTTGTTTATTTAATGATTGCAGCAATTATTTTTGGTTTTTCAATCAAACCTCTATCAGTCGTTTATGCTAAAGAACAAAATGAAGCATATATTAATTTAGATGCAGAAATACCAGATCATTTTTATATGTCAATTCGTCAAGGAATTTTAAATAATCCTCGATTAGTATACTTTTTAGGGGCACAAGCCCATATTGATCCAAGATTGATTTTAGCACACTTAACAAACCATAATCCTGAGGTATGAAAAGTTGTTATTAAACAATTAATAACTCCAATTTGAGCAGCAGGGCCTAACTTTAAATATTTATATGGTATTGATAATCCAATTACCACATCTTTTGTGCAAATTTCAGCAGAGGTAGTTAAAAAATTATTAATTGGTGATAATACATATATTCATTTAACAATATTTGCAATTTTTTACTCATTGTCATTAACATTAATGCGTTATCGTGATCTAATTGCTAAAAAGCAACAATCACCTTTTCCAACATTATTATTGCAAGTTTTAGTAATTGCTTTTGTTGTTGGTTTTGGTGTTGATTACCAAAGTAGTGTTAAGTTTGCTGGATTATTGGCGTGATCAATGCCATTATCAATATCTAGTGCTGTAATTTTAATTATTTCTTTATTAATGTTTACGATTGTCTACTACAAGTATTTAAATAAAAACGATTATAAAGATAATCAAATTACTAATCAATATTCATTCAAATGAATCCGCCATATTTTATGAAAATAGAATTTAGCAATTATTAACCTTTAATTCAAAAAATAAAAACATTAAGAAATACATTTATTAGGGTGTACTTCTTAATGTTTTTAATCAATTAATTTATCTTGTTTATTTATCACGATAATTTCGAACGTCTTGGAATTTACCATCTTTTGTTTGAATATAAACAACAGCATTTTGATTACGTCCTAATGTTTTGATATATTCTAAAGCTTCTTTTTTAGTGCTAAATAATTTTGTTGCACGTTCGCCACCTTTTTTAAAGATCTTTCAACCCTTATCTTCATCAGGTGATAAATAGTAAACTACTTTATCTTCAGATGCCATAAATAATTATTCCTTTTAACTAAATTTTCATATTTATATTTTATAAAATAGAAACAATGAAATAAACAAAATTATAATAATTGGTCAATTCAATTATTATTTTCATTTGGTTTAGAGATTTCTTTAAAAAAAGTAAAGCTTGTATTGAAATTAACAATATGATTAACATTAAAATTCAACGGTCTTTTTGTTATAAATGTGATTCTTAAATCAATATTTTTGTTACGATTTCATTCAACTTTTACATCAATTATATCTTTTAATTTTAAATTTTCATGTGTATATTTCTCATTAAATGATGGTGAATTAATATCCTTAAAAATAAAATTGTATAAGGTATTAATTAAATCTTGTTTATTTTTAGAATATCCATCAACTGTTTTTAGTGATAAAATATTTTTATCACCATACTGATTATCATCACTTAGAAATTGTTTTTCAAGACTTTGAACAAATTTATCATATATTTCTTTATTTTCTTTTGATAATGCTGAATAACGTAAATTAGTATTAAAATTAGTGTCTAAATATGTAGTTTCATTTGCAGTATATGCTAAATAATATGCTAATAAATCTTGAGATAATCCATTATCTTTATGAACGAATAAAGGGCTAAAATTACCATAAAAAGTTGTTTTTAGACCCTCTTGAATTTTAAATCCACCTCAATAAATTCCAACATAGTTATTATTGTCATCAATTATTGGACTTCCTGATGCTCCTGAATGTAAATCAAAATTTTTTGTTGCATAGTCATAAGCTTCATATTTAGTAGGATTATTTTTACCTATTGTGTTACGGTTACTAACTGTCATATATTCTTCATTATAACTAAAATTTATTGGATTATCCTTCCGATAATTTGATGTTGAAAATGAATTAAATAATTCATCTGGTTGGTTTGAATACTGATTTCAACGACTATATTTTTCATCATGTGGATATCCTGCGGCATAAAACCTTTGATGATTGCTAATAGTATTACCAAAACCAATACCTACTTCTTTATCAAAATTTCTCAAAGCATCAATATTATTTTTTTGTAAGATATCTGGTGTGATTTTGATAATAGCTAAATCAACAGCAGTTGTTAAATTATTTAAAGAAGCTAAAGCGGCTGGGTTTTTAATACCATAATTTTTTAGTTGGTTTTTTAATCCAACAGGCAAAATTGAAAAAGTGCCTACTGGATTATAATTTACATAGTATTGTGGATTATAAGTTTTTTTAGCGGATTTAAATGATAAATTTTCATTAAAAAAAGCTCTTGTATCTTGGGATTGGGATTCTAAATCGTATTGACCAAGCGTTAAAGAAGCATTTTGTTTTGCTGCTGAAATCAAATTAATAACATGAATGTTAGTTGCTAAGTAATAAGTTCTAATGTTTTTTGTAAATGGATCTTCTTGAAAATCAAAAATTCATCCTGTTCCAAAAATATTAATAGCTTGATTTTTATCTAAAGGTATTGTGAATTTTAAAGCAATAGATCTTTTATTTAAATAAGCAGTACTTACATTGTAATCAAGATTAGATTTTAGTTTACCATGAGTAAATTCATTTTGTTTCTCATTATTACTTTTGAACAAATCAATTAGAGAACAACTTGTTGTTATTACAGGTATACTAATTCCTGTAATAAGAATCAACCCTGAAGTTCGAACTAGTTTTCATTTTTTCATATATCGCCCTTTAATTGTTTTATATTTTGTATATTTTACTATAAATCTGATTTATTTTTTTTAACAATAATATAAATAAAGTAAGGAGAAATAATTAAGGGAATGGCTAAATTTAGTCATTGACTATCAAAATTTAAAATTTTAATAAATAATAAAAAAGACAACATATATATTAATGACCCACAACATCCTGAACTAATAGCAGCGGATTTAAATCGGTTGTTACTAATAACAGAACCCATGTTTCCAGCCATTAATCCTATAAATATCAAATTTCCATTCAAACTATATGATGCTCCTACCATTAAACAAATTGCAACTAAAAGAAGTCCAGAAGTTAATTTAACGTTGATTCCTAATTGATTAGCTAAGTCTTGATTAGATGAAACTATTTGAATTTTTTTAAAATTAAATAAAACTATTAATAGACCTATAATAATTAAAACTAAAGCGATAAAAAAAGACTCTTGTTGTGGTTCAATACTACCATATGTGTATCTTGAAACGTAAGCATTAGCTAATTTAGGTAAATTTTTAGCAATACTTGCTCCTAATGCAACGCTTAAAAAATTGACAATAACTCCTGAAATCAGCATTTTTTTATAATTAAAACTACTTTGTTCTTTGCATGTAAAATAATAGAGCATACATAAAATAATTGGTGCTATAAAAAATATTATTGGTTCAGTATGCTCTAATGTTGATAATTTATCAGCACTAAAATTATTAAAATCAACCTGAAATGCAATAATAATTACTCCTAAAATATTAACACTACTAATTCCCATAATTGATGTATCACTGAAGCGGTTTTTTGTTAATTTTTGAATTAAATATGCACAAAATCCTAAAATAAAACCTGATAAAAAAATTTGCATAATTATATAAGCCAAGGCAATATTATCTTGTTTAAAATTAAAATCATAAGTTCAAGCTAATGTAATAATAAATACAACAATACTTATAATTACTGTTATCATTGTTTTTAAATATGCGATTTTATTTTGATATTTTAAAGGGTTTTTAAAAGAATGAGTTTTATTTCAAGTGTTTAAATTAATCATTTTATTTAATAAATCCTTTTCTCTTACTTAGAATGTAAGCAAATACTGGTATAGCAAAAACACAAATAATCATTTCACGGGCACTAGGAATATATTCATTAATGTAACTTGAAAATGATAAGATTCCACTACCAAAAAGTCCTGCTAGTATCATTAGTAATAAAACGTGTGTTTTTTTAAAAATTGTTTGAATAATAATTCCAACAATTACTCCTAATAAAGCAATTATTCCCAATAATATAGATGATGATATAGTAATAGTTACCACACAAATAGCCACTAACCAATAAATTAGATTGACTTTAATCCCCAATGATTTAGCAAGTATATAATCTTTTCTAATAATATTTAAATAAGGAGATAAAAATAATAAAATAATAGTAGAAATAATCATTAGAACACTTGAAACAATCATACGATAATTGTTTATTAATGTATTGTTGATAGTAATAAAACGCCATCATCCCACAATTTTTAAATTGTCATGTGTTGCAATTAAAACAATATTAATTCCCGTTACTAATGCTCCAATCCCAAAAGCAACTAAAATAGGTTTAAAACTATTTTCTAAAAAATTATTTCTCACTAATATAAAATTAACACCTATTATAATAAATGAAAAAATTAAACCTAAACAGTAAATTAAAATTGTTGAAAAAATACCACTTGAAGTAATAATAAATGAAATGGTTGATGCTAATATTGCCATTGGTGTAAAACCAAGTGTTGACGGTCCAGCTAAAATATTTTTAGAAGTAATTTGGATAGAAATTGAACCAATTCCTAAACTAAATCCTGTAATTATATTAGCAACAGGAACAAAAATATAAGTTGAATTTGAAAGAGAACTATTTTCAAACATTCTTTTAATGACGTTCAAATGATTACCTGTAAAAAATAAATCAATTAAGAAAAATAATGAACAAAAGATTATTAGAAAAACAATAAATATGATTTGTATGCCTTTTAATCTATTTCTTTTTTTGTTAAAAGATTTTAAATTATGCATTTTTTTAAAATATAGAAACGAAGATTTAACATTTTTTATTTTATTCATAATTTCATCTCCTATTTACGCTTATTAAAAATATTTTTTATAGTCTTTGAGATTATAAAATTTATAACATATATAATATATAGGAAGGATTTTTATGTATCTAAAAATTAAAAATTAAAAATTTTAAAAAAAGATTGACATTTTTTAAAAAAATAACTATAATTTTATAGTGTTTTTAAAATTATTATTAAATGGCTACATAGCTCAGCGGTAGAGCAACCGGCTGTTAACCGGTTGGTCGTAGGTTCGATCCCTACTGTAGCCGCCATGGCCTGTTGGTGAAGTGACTGAACACACACGCCTTTCACGCGTGCATTCACGGGTTTGAATCCCGTACAGGTCACCATTTATAAAAACACGAAGTAATACTTAAAAATTTAAGTATTATTTGTGGAGTGCTAGCTCAGTTGGGAGAGCATTTGCCTTACAAGCAAAGGGTCAGGGGTTCGAGTCCCTTGCACTCCACCATGCCGACTTAGCTCAATAGGTAGAGCAACTGACTTGTAATCAGTAGGTTGTGGGTTCAATTCCTATAGTCGGCACCATTATATACTCAACCTGCTTATTAGTGGGATTTAGCTAAACCCCTATCTACTTTGGGTAGGTAGGGGTTTATGCTCTGTTAGCTCAGTAGGTAGAGCAACTGACTCTTAATCAGTGGGTCGCGGGTTCGAAACCCTCACAGAGCACCATTAAGCGCTTGTGGCGGAATGGCAGACGCGCTAGACTTAGGATCTAGTACTTTGCGGTGTAAGAGTTCAAGTCTCTTCAAGCGCACCATTAAATTATCTAAAAAAAACACTTTTGAAAGTGTTTTTTTTAATGTATTTTTATTCATTTAATCAATATATATTATGAAAACAATAATAAATTATATATAATATATAAGATATGCTAAAAACATGTATTTAATAAAGGTGAATATAAATGACAAAAGCTGAATTAGTTAAAGAATTAAGAATAAGAACACAAGCTAGTATGAGTGAATGTATTAAAGCTTTAGATGCGTCAGAAAATGATATTGAAAAAGCAATAGTATGATTACGAGAAAATGGTGCTATTAAGGCTGCTAATAAACTAAAAAATGCAGCGACAGATGGTGTTGTATTGGCCAAAAAAATAAATAATAAAGCAATTCTTATTGAGGTTAATTGTCAAACTGACTTTGTTGCTAAGAATGAAAATTTTTTAGCTTATGCTAATCAAATTTTAGAAGAAGCATTAGCTAAGGTTGAAAATAAAGAAGATTTTGATAAATTAATTATAAATGGAAAACCAATTGCTGAATCAGGATTAGATTTAACAGCATATATTGGTGAAAAAATCGTTTTTAGACGTGGAGAAATTTTAAAAGCTAATGATGACCAAACTTTAGGTGTTTATACTCACAATAACAACAGAGTTGCTGCTATTATTTTAGTTGATGGAAAAGTTGAAGATGAAGTTGTTCGAAATGTAGCAATGCATGCTGCAGCAATGCGTCCACGTTATTTAAATGAAAAAGTTGTTGATAAGTTATGATTAGCGAAAGAACGTGAAATTATTGTTAATCAATTAGAACATGAAGGTAAACCAGCAGCTTTTGCTGCAAAAATTATTGACGGGCGTTTAAATAAAATTTTAAAAGAAAATTGTTTAGTAGATCAATCATATTTTAAACAACCAGAATTAACAATTGAAAAATATTTAAAGAATAATAACGCTGTTGCAGTAGGTTATTTCTCATATGAAGTAGGCGAAGGAATTGAAAAAGCTCCTCAAATGAGTTTTGCTGATGAAGTAGCAGCACAAATGAAAAAATAATTTTTAAATGTAAAAATAGAAGGAAACAAAGTTTCTTTCTATTTTTATAGTGTAGTCAATAAGGAATATGAAAGGAAATTCATATGCGAAAACAACGAATTGTAATTAAAATTTCTGGAGCTTGCTTAAAACAAAATGATAGTTCAATAATTGATTTTATAAAGATTAATGATTTAGCAGAACAAATTGAAAAAATTTCTAAGAAATATATTGTATCAATTGTTTTAGGTGGTGGCAATATTTGACGAGGTAGCATTGCCAAAGAGCTAGATATGGATCGTAATTTAGCTGATAATATGGGTATGATGGCAACTATTATAAATGGGTTAGCTTTAGAAAATGCTTTAAATCATTTGAATGTTAACACTATTGTTTTATCTGCAATTAAATGTGATAAATTAGTTCATGAATCATCTGCTAATAATATAAAAAAAGCTATTGAAAAAGAACAAGTAATGATTTTTGTGGCTGGAACAGGATTTCCATATTTTACAACTGATTCGTGTGCAGCAATTAGAGCTGCTGAAACAGAATCTAGTATAATTTTAATGGGCAAAAATGGTGTTGATGGTGTCTATGATAGTGATCCAAAAATTAATCCTAATGCACAATTTTATGAACATATTACATTTAATATGGCCCTTACTCAAAATTTAAAAGTTATGGATGCAACAGCTTTAGCTTTGTGTCAAGAAAATAATATAAATTTATTAGTTTTTAATATTGATAAACCAAATGCAATTGTTGATGTTTTAGAGAAAAAAAATAAATACACAATTGTTTCAAAATAATTTTTTAAGGAGATTTTATGAATTTTAAAATTTATGAAACTAAAATCAGAGAAGAATTTGAATTAGTGTTAAAATGAATGCACAATGAATTTATTAAATTGCGAACAGGAAGAGCTACGCCTGCTATTTTAGATGGTATTTTAGTTAATTATTATGGTTCAATGATGCCAATTAATCAATTAGCAAATATTTCAGTTCCAGAACCACGTGTTTTAGCAATTAAACCTTATGATAGAAGTAGTATTAAAGATATTGCTACTGCAATTAACGCATCAAATTTAGGTGTTAATCCTCAAGTAGATGTTGATATTATTCGTTTAACGTTCGCTGCTCCAACAGAAGAAGTAAGAAAAAATTTAGTTAAAAAAGCAAAACAGGTTGGCGAAGAAGCTAAAATTCGTGTACGTCATATCCGTCAAGAAGCACAAGATTTATTCAAAAAAGATTCAACAACTATTGAAGACGATAAAAAGTTTTTTCAAACAGAATTAGATAATTTAACAAAAGAATTAAATAAGGAAATCGAAACAGTTGTAAGTCATAAAGAAAAAGACATAATGACTGTTTAATTAATTAAAAATGATTAGTGTCAATAAAATCAATGAAAAAACAAAAAACACTTTTTTTAAACGGTTTTATTCATCGATTTTTATTTTTTTATACTTATTAATTTATTTTGTTTTAGCATTATTAGCAGATCGTAATTATAATTGAACACCTTTAATTAATAATTTATATATTCAACAAGGGATTGCAATTGTTTTATTAATATGATTATTACCATTAATATGTATTGGTTCGTATGAAATGAATAAAGTCATTTTTGATAATAATAAGATAACATTAATCATCTTAACAATTGTTTTTAATATTTGCATTTATACTACAACGATTTCTTATTTTATATATCAATATGAGTTTTTAAATATTAAATACCTTTTAACTATAAATTACCATTACGTCATCAAACTATTTGGCGCATGTTTAGGATGCTCGTATTTTGTTACATTAGTTATTTTATTTTTCTTTTTAGCATTTCTAAAAAAAATTAATTTCAAAAATTGTTTATACACAATTTTAATTTTTAGTTTTTTAAGTGGTTTTTTTATAGGATTACTTTATTTTACTTTTATTAGAAGTTGAATTACTTCATTATTGCTAATGATGATTGTTTTTTTAAGTGATACATTTGCATATGTTGGTGGTGTTTTATTTGGTAAAACAAAATTAGCACCAAAAATAAGTCCTAACAAAACAGTTGAAGGATTAATTTTTGGTTTAGTAATTGCAACAATTGTTATTATGTTAATAATTTTTGGTTTGTCATATGTGCCAATTAAACCTAAAAACGGCATAGTGTTAAAATCACAAAATGTTTTATATAATATTTTTGGTTTAGATTTTTCTAGTTCAAATGAAAAAAATCTTACATTGCATAAACAAGCATTATGATGAATTTGCACAATAGTTGCTTTTTTGTTTTTGAGTTTAATAAGCACTAGTGGGGACTTAGTATTTAGTGCGATTAAACGTAATTATCATACCAAAGATTATAGTAATTTAATTCCTGGCCATGGTGGATTATTAGATCGTATAGATTCACATTCATTTGTAATATCAATCATGTTCATTTTTACACTTCTTATTACAGGCAGTTTACAAGATGAAAATTTATTTCCTAACATTCAAAAATTTTTTATTCAATTAATTGTATAATTTGAAATTATTAATTATAGATTCAATTAATGGTTGAGTATTAGTGTATATACTAATATCAATCATTTTTTGATCTGTTTTTAAACTAAGAAAGATTTTTATTAGGCAATAATCATGAGTTTGTTTTAAAAATTTAAATTTAACATAATAAGTTAAATTTTTAATTTTAATTTCTAGTTGTTTTTTGGTTTTAATTTTTTCATAATTGTTTTTAGTGATTATAGATAATTTTGTTTGAATGTTTAATGTCGTTTTAAAATATTTTTTATTTTTAAATCCAAACATTACACCAATTGCTATTAATAAAGATAATAAAAAACCCAAAAGACTAATACTTATCCATTTTTTCATCATTTAAATTAATTATTTGATCAAAATATTTTTTTAAATAATGATCATGTGAAACATAAATGACAAAATTATTTTTTAGAATTATAGGCAATATTGAGTTTAAAATTATTTTTTTATTATCATTATCAACATGTGCTAGTGGTTCATCAAGTAGTAATAATTTATTTTTGTATTTTAAAAGATTTAAAAAAGCAAGGATTTGTTGTTGACCAGTTGATAAGTTAATATTATGTTCAAAATTATTATTTAATGATCAATCGATCTTTGTTATAAAAATAACTATTTTTAAAGCATTAATAATTTCTTCATTTTTATCTATAAACCAATTTATATCAAAATCATTTATTAAAGGATTTGTTGGATGATAGATAATTAATTCTTTAAGTTGATTTAAATTTATATTATTTACATTTAAGTTATTAAAAAATAAATTTTTATTATTAAAGTTTAAAAAATTTTTTAATAATGTTGTTTTTCCAACACCACTTTTTCCTATGATTAAACAAGATTTGCTAAATTCAAATTCCTTTTGGTTTTCGGTAATTTTACATGATGTAATTTTATTAATTTGTATACCATCATGTTCTTCATAATTATCAATATACAAAATTTTTTCAACAATTTGTTTTCCATTTTTAAAATTTAATAATTCATTAAAAAAATTAAAGATACCAAAAAACAAATTATTTAGATTTTGATGTAAAAAAACTATATAAGTGATTACGCTAATAACTTTATAATCATAATTGGTATTAATGATTAGATAAGTTAATAAAATCAATAAACCAATATTATTAATAATTGATAAATAAAAATTTACATTCGATTTAAAAATAGTAATTTTATTTTTAAAAATTATTAATTCATCAAATTGCTTTTTATAACTATTAATATAGTTATTATATTTATCGATATTGTATTCGAGATTAATTAATTTAATAAAATTTTGATATTTAATAAATAATGAGTTTTCAATATTAATTATTTTTAAACTGTGGATTTTAAAATATTTTCAATTAATCAATCCTATTATAAAATTTAACAAACTAAACCCTAAACTTATTAATAATAAAGATCAATTTTGTAAACCTAATAAACAAATAATACAAATTGCAAATAAAGTATTAACTAATAATTTTATACGACTAACTAATAGAAAATGAATTATATTATTCAAATGAATCTGAATCGAGCTTAATTCACCTAAATTTGTTTTATTAAAAAAAATAATTGATTTATTAGCTAAATTATTAACTATTTTATTATTAATATTAGAAAAAATATTATTACTAATTTTATTTATGTATATAGTAATTAGTAAATCTTTGAAAGCATTACTAATATAAAATAAACTAAAAACAATAAATAAGTATGTTAAGTTTTTAATTTGTGTGTCAATTAAAACTAAATCTATTAGTAAGTTTACAATTTTACTTAAAAAAATATTTAAAGGAATATTTAATAACTCTATAAATAATATGATAATTAACGATGAGAGTTTAATATTTTTTAAATAAGATTCTTTAAATATGGGAAGGTCAAAAATTTGATGATTTTTAGAAACACGCACAATTATATTTAAAAATATTTTTTTAAATTCTTCATTATTTAATTCATATATTTTATTTTCTGGATCATAAATACGAAAAAAATTTTTGTTATAATTTTTAACAATAACAAAATGATTCAAACCGTTTTTGTTAATTAAAGCTATAAAATAGTCATTGATTTTTTCATCAATTAACTCCTCAAAACTACATTCAAATGATTGTGCATCTAAATTAAATTCATTTGCTAAGTTTTCAAGTTCTTGCAAACTCAAACCATTTTTTGTTAAATTTGCTTTTTGAAGAATGATGTTTTTATCAATTGTTTTATTATGATAATAATTTGCTAACATGTTAATAACACACACACCACATTCATTATCAGAAGTTTGTTGAACAATTTTCATTATAATCACCTCTTTTAAATAATTAGTTGAAAATAATGTTCTTTTAAAAAATTTATATTTATATCGATAAGATATAATAGAGTATAATTTAAGGATTAAAACACATATTATTTTACATTTTTATATATAAAGGAGCTTTATGGCTAAAATTAATTTTTTATCGTTAGGAGGACAAGATGAAAGAGGAAAATCTTGCTTTGTTCTTGAAGTGAACGATGATATTTTTATTTTTAATGCTGGTGCGAAAATTCCAACAAGTGATGTTTTTGGCGTAAATATGATTGTTTGTGATTATTCTTATTTAGAAAAAAACGCAAAACGCGTTAAGGGAATTTTTATTGGTACACCAACATTTAATAATGTTATGGGAATTAAATTATTACTAGGACAAGTAGGTTATAAAATCCCAATTTACACAAGCCCAATAGGAGCAATTGTTATTAAAAAAATTTTTGAACAAAAAGTAAACAATAAAAAAATTGAACCTAATATTATTGAGCTAGATCCAATTTCAGATAAGAAAATTGGATCAATTTATGTTACATCTTTTAAAGTTTCAAACTCAATGCCTCATTCATATGGATTTGTATTAAAAACAAGCGATGGAGCAATTGTTTATGTTGATGAGTTTATTATTTCAAATGATAAAAATAAAACCTTTGATTCACAAATTAATCTTTTAAATAACATTACTAAAAATAATACTCTAGCTTTGATTGTAGGAATGGGTCAAGCAGGAAATCCTTATTTTACTGCTCCCAATCATAAAAATAAGGCTTTTTATGAAGCTGCATTGCAAAATACTAAAAATCGTTTAATTGTTGGTTGTTATAGCAATGATGCATATAGTATTTTTACTTTGGCAACAATTGCAAAACAACAAAATCGCCCCTTTATTGTTTATAGTAATAACTTCATTAACACTTTTATTGGTGTATTGAAATTGAAATTATTTAATAGTAAAAATTTAATTTCTTTACCGGTTTCTGAAATTAATAATTCAAAAAATGCAATTATTGTGGTAATTGAAAATCAAGATACTCTTTTTTCAAAATTAAATAAAATTCTACATAACGAAGATAAATATGTTAATTTGTCTTCTGATGATCAACTAATATTAGGCGTTGTTATTACACCAGGTTTTGAAATGTTAGCAGCTGAACTAAGTGATGAAGTAGGTCGTTTAGACATTCCTTATAAAGCATTACCTAAAACAGTTTTACCAATGACACAATCTGATGAAGATTTAAAACATCTAATTAATTTTTTACAACCAAAATTTTTAATTCCAATTAATGGATTATATAAAACTGAGGTTAAATTTAGTTCTACAGTTACAACATCTTGAATTAAAAGTGATCAGATTATTAGTGTTTCTAATGGCGAATTATTCACTATAGAAGATAAAGTTTTAAATCCTAAACCCCAAATTATTGAGTTAGAAGATAAATATATTTCATCTTTTGATGCACTTGATGTTGGTGCTAATATTTTATTTGAACGATCACAAATGGGCGAGAATGGTGTTATTAATTTAATTGTTATTTTTGATAAACAATTCCAAAAACTATTTAATTATGTTGAATTTGATTATTGTGGAGTTGTTAATAATGACGCTCAAATTAAAGAAATTGAAGAAACATTTAAAAAACGTATGGGTGAATGTTTAGTTTATGATGAACGTAAACGCTTAATACTAAAAGATACCAAAGCAAATTTAAAACGTTTATTAACTAAATTATTTGAAAAGAAATTTAATAAGCGTCCATTAGTTTTGCCAACAGTAGTTGACTGTTATAAAACAAAATAATTTTTAGAGGTAATGTATGGTTAGTGACAATAAAAACACATTAAATAAGGAAACAATTAGTTTTTATAGTTATGACAATCAACTAGATGCACAAAAAACAGAATCAAACGGAAAAGAACAAGAAAAAAGCAATTTAAAGACAAAAAAATTCAAAGTTCAAAAAAATTATTTTTTTAATAAAAAAAAGCTTATTGGAATTTGTTTTTTTATATTAAGCTTTTTAATTTTAATACTTGCTTTTTTAAAAGCCCCATATACAGGAGCGATTTTAGACAATGTTTTAGAATTTTTTTTTGGATGAGTTAAATACTATGTTTATGTAATTTTATTATTATTTTTAATTGTTTTTTATATTAAAAAAGTCAGAAGGCGTTTATTTAGTAAATGAATGATTGTTTTTTATATTATTACACTAATTTTATTTGCTTTAATTATTGGTGCAGTTGGTTATGGAATTATCACAAATTCATTAACTTATAAATTCATTATTAATACAAATCAACAAATGATTAATAAGGAAATACTTAATTTACATAAAGATAATTTCGAATATATTAGTTTAGTAAATTTACACTCAAAAAAATTACATTTATGTGAGTGGTGAGCTTATAGCTGATCGCAAGAATTTTTAAAAGCGATTGATGAAAAACATTATTATTATACTAGTGTTTTTGCTTATGGTGGAATTATAGGATATGCTAATTTAGATTTATATCGTGCTAATGCTTGAATTTTTACTATTGTAATTATGTTTATGGTTTTAGTTCTTATTTCTTTTATTCTAATCACTTTTGGCACGAAAAGTCATTTAGGATTAAAATTCAAAAAATGATTAGTTAATAAAATAATCACTAATATCAATAACTATCACAAAAAAGATTATCAATTAAGTGAACAAAACTTTTTCGAAGAAAAACTTGGTGAAGATCACCACAATATTGATAAACAATTGGAGCAAAAAGAAGTTATCCAAGAACAAAATTTACAAATTAAAAATTTTAATGATCGGCAAACTAATTCTTCATGTATCACAAAAAATGATAAACTAAAAACCACTATTTTAGAACCAATTGTTAATGATATTAACCAATTCCGAGAAAATCCATCAACAATCAAGAATAACAAGTACAATTTTTATCCACGAATTGATATTATAGATGGCAAAAGTCAAGATTATTTGCATGAACTTAAATCAAAAGGTGAAAATTTAAAATTAATTATCGATAAATTTTTAGAAAATAACCAATTAGAATATCATTTTAATGCAATTAATCCTTATTTTTCAAATGTTGAAATTGTTTATCAGTTTACTCGTTCATCATTAAACAATTTTTTAAAAAATTTTTTAAATTTAATGAAACAAGCAGTTAATGATACAGATGATTATGAAATCAATATTTATAATGACAATGACTTTTTAATCATTCAAGCCAAAGTCAAAGATTTAAATCCTCAAATTAATATTAAAGATATTCTAACTAATTTAGAATACAATGATAAATTATGTTTAGGAATTGGAAAATTAAAAGAAAGAAAAGTAGTTTGATTAGAAGATACACAAGCTGGAAGTATTTTAATTCATGGTAGTCAACAATTTTCTGGAAAATCAATGTTAATTTCTAACATTATTATTAGTGCTTTATATACTAAATCACCTAATGAATTAGAATTATTTATTATTAATAATGGTTCAAAATCATTAAAAGAATTTGCTAAATTAAAGCATACAAAAAAAAGCGTTGATCATGATGATTTTGAAAATGTTATTAATTTATTAAGGGAAATTATGAATGATATTAACAAGCAAAATACATTATTTGCTGATAATAATGTTGATAATTTAGATGAATATAATCTAAAAAATCAAAATCAAAAATTACCCAAAAAATTAATCATTATTTCTGAATATGTTGAGATAGTAAGTAGTCAATTTAATACACGTTTTGATACCTTAATTCGCAATATAGCAAATATTGCTAAAAAACACGGAATCGTTTTAATAATTTCATCAAATATAACTAACGAAAGCACAGTTTCTTTTAAAAATGTATTTGATTATACCATTGTATTAAAATTAAATAATCCGTATGAAAGTATTTTATTAACTGATCGTAATTGATGTAATAATTTGGTTGGTTTTGGGGATATGTTATTAATTCGCAATTTTGATAATTTGCCACTACGATTGCAAACAGCAAAAATTACAAATGAACAGTTTGCTAATATTATTAATGAAATCAATAGTGCTGATTATGATATTGATGAATATCGTCATGAAACAAGATTAACATCAAGAACCCAAAATTTTTTTAATAAATAATTATATTTGTTTATATTAAAACTAACATGGCACATAATTTAGATAAAAGCATTATAATAATATAAGAATATTAAAATTAAAACTATCAATATTAAAGAAGGAACGGAATTTTATGACATTAAATGAAAAAAAAGAATTTGTTTATGAATTAAAAGCTGTTGAAAAAAATCACAACCTAAAAGGTTTTGAAGAAAATACAACTGAACATGTTGTTTTTGGAGGTTTATATCAAAAACAACAATACTTAATTTTAACACCAGATTTTGATTCTAATGAACTATCAAGATCATTGATTAGTTTTTTAGAAAAATCACCTAAATCTGTTAGTGTTGATTTAAATTCATTTCTATCACTTGTCCCAGAATCAAGACATGCATCTTTATTAAATGTTGTTATTAGTGCATTAGAATATGTTGAAGTAACACCATTTTCACTAAAATCTAAAATTGAAACAAAAAATATACATAATTTAGTAGTTGATCAAAAATATCATGATTTAATTAATAAATTACATGTAATTGCTCAATCACAAACAATTACAAGAACATTACAAGATACGCCAGCTAATTTAATGACACCAGGTGATTTTGAAGAACGAATTAAAGAATTATTTAAAGACTTGCCAGAAGTTAAAGTAAGTGTTTTATATCGTAAAGATTTAGAAGCTAAAGGAATGAACGCTCATGTTGGTGTTGGTAAAGCTGCAGTAAGTGATAAAGCACAGCCTCGTTTAGTTGTTGTTGAATATAATAATAATCCAGAAACAAATGAAAAATATGCTTTTGTTGGTAAGGGTGTATGTTTTGATTCAGGTGGATATAATGTAAAAACGGGATCACATATGCGTTGAATGAAATTTGATATGTCTGGTTCTGCTATTGTAAGTATGACAGTGAGAGCTTTAGCTTTAAATAAAGAAAAAGTTAATGTTGTGGCTGTATGTCCATTAGTATTAAATTTATTAGCTCCAGAAGGTCAAAAACCAGATGATATAGTGAAATCATATAATGGTAAAACAATTGAATTAGATAACACTGATGCAGAAGGACGTTTAATTTTAGCAGATGCACTAACATATGCTGTTCGTGATTTAAAAGCTTCTAAATTATTTGATGTTGCAACTTTAACAGGTGCTATGATTTATGCTTTAGGTGATACATATTCTGGTGTATGAGCAACAAATAACGACATCTGAAATGAAGTAGTTGTAGCAGCTGATTATGCTGGTGAATTAGTTTGAAGATTACCATTCCACAATGATTTCTTAAAAATGTTAAATTCAAATGTTGCTGATATTGCTAACTCAGTATCTGATCCTCGTGGTGGTTCTTCACGTGCAGCATGTTTCTTAAAAGAATTTACTGAAGGCGTTCCATATGCTCATTTTGACATTGCTATTACTGCAGATGTTGGTCATAAAGGAACTGGTGTAATGTTAAGAACATTTTATCGTATTGCTCAAAATCAAAAATTTAATTAGTTTAAATTTAGATAAAGAAAAAGAAGTGAATTTAATTCGCTTCTTTTTTATTTGTATCATAATTTACGAGTGTCTATGTATAATTTTGTAGAAGAAGAGGTTATGTTGTTTTTTTGTATAATAAAGTTTAGAAGTTTTTGTTTGATCAGAAGATGCTAAAAAAGATTTAATTGGATAAAAATACAAATATTTGACTCTTTTTTAATATTCAGAATAAAGGTAAAATATTTACTCTAGATATATTGAGGATAATATGAAACAATAAACAATTAAATTAAAAGATGTTAGTATCGTTATTGGTCACATTTTGACAAAAACATTTTTATCTTAATTAAGAGATATTTTTAAAGATTACCTATATGATCAACAAAAAGTTAAAAAAAATCTCGCTGGAGGTTCAAATATCTTGATATGTTAATTGAAGTTTTGTTTTATTTAGTTGTTTAATGTAGTAAAAATATAACGATACAGCATATAATATGATTGTGCCATAAGAGATAACTGGTGAAGGTGTTGAATTAAATTCAGTTTCAATTCCAAATGATTGATTTTTTAGGCTAACTTCAATTTGATACTTATTTTATATGTCACTGACTTTTCTCTAAATGGTAAAGTTGGTTGGTTAATTAAATTTTCTATGCAATTAGCAACTATTAAATCAAGAAAACTATCACCTATTAAAACTATAAATATAATAATTAGCTATGGTCTATAAATTAAGATTATATTCAAGTAAAAAAATACTTTTTTTATGTTATTTTGTGAACTTTAATACTTCTATTAATTGTTTAACTACTCGTCTTACAAAAACAACTTATGTGAATTTTATTGATTGCTTATGTTCGATTGTATTAGAAACATTTATTGGATAGTTTGAAAATATAATTTTATCAAATTAATAAATAAAATTTTTTTTCAAAAACAAAAAAGGACTGATACAAAAAGTGTCAGTCCTGTTGTTATTATATGGCAGGGGTAGCAGGACTCGAACCCACAACACGCGGATTTGAAGTCCGCAGTTCTACCATTGAACTATACCCCTAATAAATGTATTAATAAATTCATACATTTAAATATTATACTAAATTTTTTATTAATACTTAAATTATTTTAATACATCTTCAAACGCGTTAATTAAATCACGTAAATTTTTGATTTTTAATAATTTTTCATCAGGCAATTGAACGCCAATTTGATCCTCAATTTTCATTATTAAATTCATAGCACTTAGCGAATCAATTCCTAATGATTTTAATTCGATATTTAAATTATCCATATTTAAAGCAATTTTATTTTCCTTAGCGATTTTCATGATTAAATCTTTAATATTAATAGACATATAATTAATCTTTCTTTTAATTAATACTTAAAACAAACTCTTCATAATATCTTGTTTCATTTTTAATATTATAATTATCGAATAATCAATTAGCAATAACAATTATGTTTTTTGCATCTTTATTAAATTTATATTTGATATCAATTTTAATATACTGCATGATATTACAAAATTCTTGAAAACTACTTATAGCAGATTTGACTATTTCATAGATATTTTTAATAAACAACTTTTCATTAAATTTAATATCATTATTAATGTTTTCATTAGAAAAATAAGTTTTGAAATCTAATAATTGGTATAAATCTTTATTTTTCGCAATTACATTAATAATATTGAAGCGTTTTAGTTTAAAAGGTTTTTCTTCATAACTTATATTATTATTTTCGTTGATTTTATTGTTTTTATCATTATTAGAAATAAAATCATTATTTTTTAATTTAGTTGAATTTTCTTGTTTGTTTTTATCATCTACATTTTTAACATTAATTATTGGTTTATTTAGATTTTCGTTAGTATTATTTATTTGATCCTTATTTTTTTCTTCTTTATTTTTTAAATAATCCTTTAAAATTTCTTTTTGTTGATTTATTTGGTTAATTTTTGTAATGTCTGTCTTTTTATGGATTATACCACCTTTTTTATCAATAATTGTTATATTTTCATTAACCTTATGTGCATCTTGTTTTTTATTATTAATATTATCTTCAATTTGAGTTAAGGGTTCTTTTTTACTAATTTTATTTGTATTTTGAGTTGATAGAATAACACCTAAAATAATAGCAGTAATTACAATTAATAAACTAATAATTCCACCAACAATCACCTTTATTTTATGACTACGCCAATTAATTTTCAAGTGATTTAAAATTTTCATAATTTTCAATTATTGCTTTCTGTACACTAATTTTTTGTTTATTGATAGAATTAAAATCATTTTCGATGTTAATTAATTGATTAATTACAAATTTTTGTGGTGTTAGATAATTATTAATAACTGTAATTTTTAATTTTGCTATTTGTTTATTTGGTAATCAAATACCATTAAAACCATAATTATTATTAATAGATAATTGATCGTTTTTAAAATCATACAAGAATTTTGTTTTCATTTTTAATAAAAAATCAATAGGTCTTTGGTAATAATCATATGTAATATTAATATTTTGATAATCTTCAATTCATTCAATTTTTTTATTTTCTTTAGCTAAATTTACGTATTCTAAGGAGTATTTAATCAATTCAAACGTTTCTTTCGTAGGACGATTATTTCATGAATTATCATCATTTAAACTAAATTTTGTATTAAATATATAACGCTTATTATTTTTGATTTTTATTTCATTATCAAGTTCTTTATCTTGAATTCAAAATAATGGAATTGTTTCTCAAAAATTAGTAGGATTAACTTTTTCGTGTTTATTTAATAAAAATGTATTTTTGTAAAAATAATCAAAATTAAGATCTTGAAATTCTTTTTGTACTGCCTCAAAAAACAAACTTTTTTCACATGTATAATTAATTTGTTCATGAATTCGTTTATCATCATTAATACGAAGAAAAAAAGCAAAATTAATTTTTTTACTTAAAGTGTTCTTATTAACAAAACCAGCTTGATTAAGATTAATTTTTAAATTGGTTTTATAAGTGTATAAGTCATAAATCACTCCTCCGATTTCATACAAAGAGTGTTTAGTTTCATAAACTTCTAAATTACGAAATTCACCTATATCACTTTTTATATCCATAAAAAAGTAATTAATAGGTAATTTAAAATCTCCTGCAACTTCAAATTCAAAGTCTAAATTTAATACATTTGAATTTAATGAAGTATTAATCATGGTATTTTCACGAATAAAAAGAGCAGGACGTCCTTTATATCGTGCAAATAGCTCGATAGGTTTTTGTTTTTTATTAACATAGTAAGCATATTTTGGAATATATTTTTTATCTTGTAAATAATAATTAATGTTTGGTGAATTATTGATCATTTTTATCACCTTCTTTTTCATTAGAATGTAAGTTTTTAATTTTATTAATGTTTCATTTATTAATAAATTGCAAATCTTTAATTACCTCAGTTTTTAAGTATTGTTTTTCATCTCAAACATCTAAAAATAAATATTGAATACGGAATAATTTTGTTTTATATAAAAAAATTTGTATTATTTTTTCTGTGTTCATAAATTAATACCTCCACTAAATAATTAGTATTAAAATATTAAAAATGAAAATTAAATTAATTAATTTAATAAGATTTAAGTTATAATAAATTGTATTTGCACCTGTAGCTCAGTTGGATAGAGCATACGCCTTCTAAGCGTACGGTCTGGGGTTCGAATCCCTTCAGGTGCGCCATTGATACACTAAAATCTGCATGGTTGCAGATTTTTTTTTTTTTTTTTTTTGTTATTAAAATAATATAAAATATATAAAATAATGCACCCATAGTTCAATTGGTTAGAATATATGCCTTCGAAGCATAGGGTTAGGAGTTCGAGTCTCTTTGGGTGCGCCATTAATTAAAATAAAACTAGAGCATTTTATTTTGCTTCTAGTTTTATTTTTATATAATAGTATAATAATAAGATATGAGGTCAAATCATGTTATTAACAATTGCTTTTTATTTATCAAAGACAACTCATTATCTAAAAAAAAATTTTAATTATTTTTTAGATTTATTAAATCAAAATCAAAAAAATATTGAATTAATTATTATTGATGATGCTAGTGATTATAATTTATTTAAAACATTAAAACCATTAATTGAAAATACTAATGCTAAGATTAAATATTTTTATTTAAATGAAACACAAGGCAATGCTTATGCATATAATTTAGCAACGAAATATGCTCACGGTAAATACATTTGATATTTAGGAGGTCATGTTGAATTAAATTTAGATGCTAGTTCATTGTTGTTTTCGGTTTTAGAGAAAGATTACGATGTTATTAGTTTTAATTTAAATGATAATGTTAACCAAAATCCATCATTGGTTTTTGATAGTTTGAATAAAGAGATATTAGTTGGATTATGAGAATCAGTTTCTAATAAAATTATTGCTTTAGATTTTATTAGAAAAAACAAACTAGCTTTTTATAATGATAAATGATACCCTGCTTTATTTATTTATGATCTTTTTACCAAATTTAATTCATGAAGAAATGTAAATATTAACTTCATTAGCAATAATAGTGGTGAGGTTGGTTATAATGTTTATGATTTACTACAACAAATTAATGAATTATACATAAGATTTAGTAATGATGATTTATTAGATCTTTATAAGGACGAATTGTGTTATTGAATTACGGGAATTTGTTTTCATTCTTTTTTGAAAAAAATTTATGAATTATACACAATTAATATTAATTCTAAAAAACAAATTAAAGAACGAACAATGATTATTAGTCATGCCTTATCAAATGCTAAAAAGTATTTAGAGATTTATTTTGCTAATTTTGAGAATAATTTATATGTTCGCAAGTTTAAAATTAATATTTTAAAATATTATTTAAAATCAAAACAGGGATTAAATTAAATGAGTGATAAGTCAACATTAAAACAAGAAAATACGATCGAATATCCCGTTGTTGGTGCACTAAGGCGTGCTAGCGCTAGTGTTTTAGATTATATTTTTATTTCCATTATTGCTTTTTTATTATCTTTATTAGTTTTTATTAATAGTAATTTTAAAATTAGTGATATTTTTGTTAATAATGATGAGCTTGCTCCTGAACCTTGACGTTTATTCTTAATGGGAATAATTGCTTTTTTTGTTTATTCTACATACTACATTATTATTCCTTTATTTTGTAAATCTTATACTTTATTTCGATGAGCATTTTCGATTAGAATGATTAGTTTAGTAAAACAAAACGATAAAAAAATTTTATTTATTGCTTTAATTAAACATAGTTTATTAACTTGATTAATTTTTACCATAATTAATTTATTAGCGATGGGATTTTGTTTTGTTTTTGCTGATGCAAGTTTTAAATCAATTCAAGCTATGAATGAGACTGAGCTAAAAGAGTATTATCATCAAATTAAAGATTTAAAAGCTTATGTGCAGTCAATTTTATCTTTAAAAATTAATGATAATTTCACCAATACTACAACTATGGCTATTTTAAGTATTTTAGTTAAAAGTCTATATTCAATTATGGGTTTAGTTAGTTGTATTATTGTAATTCACATGTTTATGAATTCAAAAAAACGTGCTTTACATGATCAAGTAGCAAAAGTTGTTTTATTATGGATGCATTATGAGAAACCAAAACAAGCGCCTAAACATAAAACAAAACGTGAAGTTGAGATTCAAAAAATCGATAATTTAATTCAAGAACTAGATAAAATATAAAATGAAAAATAAGGAGAAGCTTTTATGGGATTAGGAATTGATTATTCACGCTTAAATCAAGAACAAAAAGAAGCAGTTACAGCAGACTTAGGACCGCAATTAGTAATTGCAGGGGCAGGGACAGGAAAAACATCTGTTTTAACTTTACGTATTGCTTATTTAATTACTGAAAAAAATATTCATCCATCACGGATTTTAGGTTTTACTTTTACTAATAAGGCTGCTGATGAAATGAAAGAACGTGTTGGAAAAACAATTGGCGTTTCTATTCCTTATTTAAGTACTTTTCATTCAATGTGTGTAAAAATTTTACAACAAGATATTCATTATTTAAATTATAATAACAATATTAAAATTATTGATACAGACGATCAAGAAGTTTTGCTTAAAGAAATTTTTGATCAATTAAACATTGAAAAAAAATCGCAAGTTATTAAGAAAATAATTAAAACGATTAGTAAAGTTAAAAATAAATTTTTTGATCAAAACGATATGCTAAATGAAAAAAATCACAAGTATTTAGAATTGGTTGATTTAAATGATGCACAAAGATTAGTTGATATTTATAAGATTTATTGTGATCGATGTTTTAAATTAAATTATGTTGATTTTGATGATTTGATCAATTTAACTCATAAGTTATTTATTGAGTTTCCAGAAGTTTTAGAAAAATGACAAAATAAATTTGATTATATTCTTGTTGATGAATTTCAAGATACTAATAAAATTCAATATGATTTAATTAGTTTATTAGCAACTAAACATCAAAATTTATTTGTGGTTGGCGACCCTGATCAAATGATTTATTCATTTAGAGGTGCTGAACAATGAATTATTAATAATTTTAGTCAAAATTTTAAGAATACAAAAGTAACAATTTTAAAAACAAATTATCGTTCAACTCAGCCAATTTTAAATACAGCTAATAGATTAATCGATGCTAATAACAACAATTATAAAAAAAATTTAACTGCTTTTAATACTAATGACAATAATTTACCAATTTATTTACGAGGGCAAAACCCAATTGATGAAGCTAATTGAATTGCTAGAAAAATTAGAGAATTGTTAGAAGAAGGAACGCCAGCTAACCAAATCGCTGTTTTGTTCCGAAGCAATCACTATTCTCGTACGATTGAACAATCAATGATGCGCGAGAGCATTCCTTATACAATTCTTGGCAGTAAAAAGTTTTATGAACGTGCTGAAATTAAGGACATGATTGCTTATTTAAAAGTAGTTAATGATTTAGATGAACTATCTTTTTTAAGGATTATTAATACACCACGTCGTGCGATTGGTCCAACTACTTTTGAACATGTTAAACATTATGCAATAAATAATAATTTATTTTTATTTGAAGCATTAGCTGAAGTTGAAAAAAACCATTTAATAAATAACACACAAAAAAAGAATATTTTAAATTTTGTTAATTTAATTAAAGAAATCCGTGATGAAATGGAAGATTTAAAAATTCATGAGATTTTAGAATTAATTTACAAAAAAGTTAACTATGAAGCTTATTTATTAGAAAACGAAAAAGCAGAAGATAAAATCGATAATGTTTATGAATTAAAACGCGCTATGAAAATGTATGTTGATCGTCATCCAGATGACACGATTAATGATTATTTAAATTCGATTGCTTTATATTTGAATAAGGATGGTAAAGATAGTAAAGAAAATGTTTTATTAATGACAGTTCATAATTCTAAAGGTCTAGAATATGAAAACGTTTTCGTTGCTGGTATGAACGAAGGACTTCTTCCTTCTGATCGAGCAATTAATGATGATCCAATTAAAGGAGTTGAAGAAGAACGAAGAATTGCTTATGTAGCCTTAACACGAGCAAAAAAAAATCTTTATATTTCATCTGCTTGTTGTTATGATCCATTAGCAAGAAGACAAGTATTTGAAAGTCGTTTTATTAATGAAATTGGTTTTAATAATTTAAAGATTATAAATAGTAGTTTTAAAAATAATAAACCAGAGGATATGCCATTAAAATCATTCTTAAAACAACAAGAAGAACGTTCATGGTTCGATTCAAAACAAAAACAAAAAGAAGTTGAAGATAATTTTTATCAAACAATGAAAAACGATTTTGAAATTGGTGAAAGAATTGTTCACACAAGTTTTGGTGATGGTGTTATTATTGGAATTGATGGTGATATCTTAACAATTGCTTTCAGTAAATATCGTCAACCAAAACAAATTAATTTTAATCATAAAACAATCGTAAGAAGAATAGGTTAAAAAATGAAAATTTTAGCTATTGGAGATATTTTTTCAAAACAAGGAAGAAAAGCAGTGGCCAATGAATTGCCCAAGCTAAAACAAGAGTATCAAATTGATTTTGTAATTGCTAATGCTGAGAATACAACACATGGTCGCGGTTTGTGTTGAAAACATTATGAAGAATTATTAGGTTATGGAATTGATTGTATTACTATGGGCAATCATACTTGAGATCATCCTGATATTTTTGAAATCCTAACAACCAAAACAAATATTATTCGTCCATATAATATTATTAATACGCATCAGTATCACTTAGTAGGTTCTGGTTCACGAGTTTTTTATTGTAATAAAAAAATGATTCGTGTAACTAATTTATTAGGTAATTCTATTGATATGAAAGGTTTACAAACAAATCCTTTTGAATCTTTAGATAAAATTATTGCTTTTAATGAAGCACCGATTCATATTGTTGATTTTCATGCTGAAACAACAAGTGAAAAAAATGCACTTTTTTTAGATTTTAAAAGTAAATTGTCTTTAGTTTATGGTACTCATACACATATTCCAACAAATGATGCTAGAATTGTCGATCATACGGCTTTTCAAACTGATTTAGGAATGTGTGGTGCTGCTAATAGCGTGATTGGGGCAAATCCAAGTAGTATTATTCAACGATTTCGTGATCCAAATAAACGTTTTATATTAGAACCAAGTGATGAAAAATATGTTTTTTGTGCTAGTTTAGTTGAAATTGATGATGAAACAAATTTTCCAACTAATATTACACCTATTGTAATTTATGAAAAATAAAAAAAGATTGACTTTTAAGTTTAATTTTGTTATTATCTTATATGTGTTTTTAAATATTTCAAAACACAAAATTAATTTAAAATTTATTAGGTTTAGTTCCAAAGAATTAATTTTATTCTTGGTGCCATAGCCAAGTGGTAAGGTCAGGAGCTGCAACCTCCTTATTCGTCAGTTCGAATCTGACTGGCACCTCCATATGCATCCTTAGCTCAATTGGACAGAGCGTCTGGCTACGGACCAGAAGGTTAAGGGTTCGACTCCTTTAGGGTGCGCCATTAAATTTTTTAAAAAGATAGTATTATGAAATATTATCTTAATCGTTCGGGAAGTAGCACAGCTTGGTAGTGCACTTGGTTTGGGACCAAGGGGTCGCAGGTTCGAATCCTGTCTTCCCGACCATTTTGGCTGGATAGCTCAACTGGTTAGAGCGCTCGGTTCATACCCGGGAGGTTCAGAGTTCAAATCTCTGTCCAGCCACCATTGCTTCTATAGCTCAATTTGGTTAGAGCCCCCGACTCATAATCGGTTGGTTACAGGTTCAAGTCCTGTTAGAAGCACCAATAAAATATATAAAATTAATGTACTAAACTATGTTATAATAAAAAGGTTGTAGTTTAGTATATACTGTGGAGAAATACCCAAGTCCGGCTGAAGGGATCAGTCTTGAAAACTGAGAGGCGATGCAAGTCGCGCGGGGGTTCGAATCCCTCTTTCTCCGCCATTATATAAATATAAAATACTTTATCAATGTTTACACACAACATTATAAAGTTATTATCGCGGGATAGAGCAGCTTGGTAGCTCGTCAGGCTCATAATCTGGAGGTCGGGGGTTCAAATCCCTCTCCCGCAACCAATGGTTCTGTGGTGTAGTGATTAACATGCCTCTCTGTCACAGAGGAGATCGCGGGTTTGACTCCCGTCAGGACCGCCATGGTTTCGTAGCTCAGTCGGTAGAGCAACGGACTGAAGATCCGTGTGTCGACAGTTCGATTCTGTCCGAAACCACCATTTAAATAAAATTAAAAATACTAGCTTTAAGCTAGTATTTTTTGTTATTAATTAAATTTTAAACGTTTTAAAGCGTGATCAGCAATAATAATATTATCAATAAATTCATTTAATAATTTAGATGCTTGAGGCACATTGTTTCAATTTAAATTTTGAAATTCTTTTTTTATTCATTCATAGTTGATACCATAATAATTTATTTTATTATAAAGCAAATCATAGATTTTTGGCATCCATAATTGGGTTTGGTTAATATATTCAATAAGATAATCAAAACGTTCATTAATTAAATTTAATTGGTGAATAATTAACTCATGATTGGTTAAAATTTGGGGATCAAAACTATTTGAAGCATTTATTTTTTGAAATATATTTTCAATATATTCGAAATCATCTAAAAAAATTTCATTAGATAAATTAAAAAATATATCTTTGTTTTTAATATAAGATTCAAGTTCGATTTTTTTATTATAAATAGTGATAGTTTTTTTAACTAGTGTATTTCAGTGGTGATTCCAATTTGCTAACTTAACATTTAAATCATTTAAATAAAACAATAAATTTTTTAAATTATCATAGAATAATTGTGTTGAAATAATGAAATCAATATTTTGTTTGATATCATTTTTATCAATTTTAGAATTAATAATTTTTTGATTAGCTTGATCTAAGTCTAAAATTAATTGGTTTAATTGGTTTAATTGATAGTTTTTTGAAGTGCTCTTTATTTTTTCTAAAATATTATTTACTTTAATAATAGTGCTATTAATGAAGCTAAAATCATCATAAAAACGTTTTTTATATTTATCAAAAACAGCCCCACCAATATTGTTTTGCGAACTAATATTAAGTCAATTTAAGGCTTGTTGATAATAACTATTTAAATAAAGGTTATTTAAATTAACAAATTTTGAATTATAAATAAAATCAGCTAATTGGTTATTAATAAAATCATTAAAATCATGGTTAAATTGTCTTAATTCTTTAATTTTGTCATCAGTCAAAAAGGTGATTTCTTGCACTTTTTGTAAGCTATTTTTATTAGCTTCTTCAATAATTTTGATTGAATTTAAAACTACAATGATATTTTTTAATAAATGAACTTTATTTTTAATACGATGAATTAATTGAACTAAAAAATAATTATACTTTGAATAATTCATACTAATTTTAGTTTCAATTAAATTATTAAATGAATCAGTAGTTCAATTAATTGCTTTTTGATAATTTAAATTTGGAATACTATATTTACTTATAAGATCTTTATACATTAAATCTAATTTCTTATGGGTATCTTCAATTTTTCTTTCTTGTTGATCAATAATAATAAAATCATTTCAAAAAGATTGAATTAAATCTACAAATTCATCTACATTATTTTTAATTTTAATTTCATTTGCTTTAATAAACTTAATAAGTGATAAATAAGAGAATAATTTATGATTTTCAAGATAATCTTGTAATTTAATTAGACTTTTTTTATGAAAATCTAATTCATTATTAATTGCAATTAGGTATTTATTAATAATTGTTATTTTTTGTTTACTTGTACGTGCATAGATATTATCTGTATGAGTATAAATATCATTAAATTTTGTATTGACTAATTGATCAAAATTAAAAAAATTTTGACGGAATGTGTTATATTTTCTAAGTGTTCAAATGTCCAATAATGCAAAAGTAGAGTAAATAAGTAAAAATAATAAAATAAAACCTATTAGAAGATAAATTAAAGACATTAACAAATTCCTTTATAGATGGTGTATATTAAATTATAATCAAATAATTTTGTATTTTTTAAATCACAAAATATATTATAATAGTTAATAGCATATTAATCAGCTTATTAGATATATCATTTGCCTATTTAATTCATATTAAAAAAATATGTTTATTAGTAAGAAAGTTGCTGACTTCTCTGAAACTATCAAAATTAAATAAAAAATTATAGAATCTAAATTTTTGTGATTATATGCTTAAAATAAATTTGAGGAGAATATAATCATGAGTCGTTATACAGGTAGTATTTATAAAAAATCACGCCGTTTAGGATTTTCTTTATTAGAAAATAATAAAGAATTTAATTCTGGTAAAAAAAGAACATACGGTCCAGGACAACACGGTAATAAAAAAGTTAAATTATCAAATTATGGCCAACAATTAGTTGAAAAACAAAAGTTAATGTTTTTATATGGATTGAATGATCGTCAATTTAGACGTTTATATAGAGTTGCACTAGGCCGTCCAGGCGTACTTACTTTAAATTTATTACAAGTTCTTGAATCACGTTTAGATTCATTAGTTTATCGTGCTGGATTTGCTCCAACACGTCGTGCTGCACGTCAATTAGTAAATCACTCACACGTTTTGGTTAACAATAAAAAGGTTAACATCCCTTCAGCACTAGTTGAAGTAGGTAGTACAATTGCTCTTAAGGCTAAATCATTAGAAATACCTTTAATTAAAAATACTTTAAACAAACCAGCTGATTTTATTGAATTAATCGATAAAGAAAAAAAAGTTGCAAAATTAGCACGTTTACCAGAACGTAATGAATTACCAGCAGATGTAAATGAAGCTTACGTTGTTGAATGATACAACCGATTAATGTAATTACTTATATTACAAGATAAGCAATTAACCAATCAAAAAAAAAAAAAAAAACGGCACCGTGCCGTTTTTTTAATGAGTAACTAGATTTTTATTCAAATTAACCTTTGATTTCAAAAGTGCGTAAATCATTAGAAATAAAATTAAATATACTTGGCGCAACATTATATCCATGTTTACTGTTTGATTCTAAACTTAAGATTTCATATTTTTCATTCTTTTTTGTATTTTCAATTTCAAATAATAGTTCAAAATTATTAGCTAATCGAACTGTTTTTTCAAAGATGACTTCATTTGGTCCTACTTTTTTATATTTAATTTTTAAAATATCATTTTCTTTTAAATTATTCTCACTATCATTAAACTTCAAATTAATTAAACTACTTGGATGATTATCAAATTTTGTATCAATTAAAACACTTTTTATTTCAACAGGTTTTGGAGCTGTAGTGAAACTTAGCTCATTATTAGTAGTTATTTTAGTTTGCGCATTAGTTAAATTTAAATCACTTTGGCTTCCAATAATTAATTCTTTAAAAAGATATTTACGGTTTGATATTAAACCATCTAAAACAAAATTACTATTTTTAATTTCAGCAATTTTTAAAGTATTTGATAGCTTTCAAGAATTATCATTGCTTTTATAAACTAATCGGGCATATTGATTTTCTAATGTTGATTGGTTAATTTCTAAATTGACATTAACATTCACTTTTGTTGCATCATTGCTAAGTGAATTAGTTTTAATATATTTAATTTCAATACCAACACTATTAGGTAAATTAGGGATATCAGGTAAAAGATTCTCAGCTTTAGTAGTGAATAATGAATCTTTTATTTGAGTTAGATCAACCTCATGTCCTGTGATATTAAACTTTGTAATTTTATATGTAGTGTTTGGTTTTAAGTTTTCTAATTTAAAACTTAGCATTTGTAAATTTTTGTTATATTCAGGATTTATATCAACATTTATCTTATTTCCCTTATCATCTTCTAATTCAATGCTGAATTTTTTAACTAAAATATCATTAACATCAAATTTAGCAAAATAGATTTCAAGGTTAATTGATGTTAAATCAATATCAGTAGATCTTAACGATGAAGCAGTAATTTGCTTTTGTTCTTTTACTATTTTTGTTTTAGTTTTTGAACACATTACAACAAGTGAACTCAAAGGGATTAAAACAAAAATAGAAATTAAAGGCAAAAGTTTCTTATGTGTGTTTTTTTTCATATTTTTAACCTATCATAAAATTTAAATTTATTATCAAATTAAATTATAACCAAAGATATTTTCAATATAGTAAAAACTTACATTATTTGTGTATTTTGTAACAAGAGAGCAAGCGGATAAATGGTATAATTTGTGTAGTTTAACAGTTTACACAAAATAAATTAGTTTTTTTTTTTTTTTTTTTTAGACTGAAAAACAGGAAAATTTTATAAAAAAGAGATGATTATTTATGAATAATAAAAATAAAAAACGTAATGCCAAAATAGCTACTGGACTTACAATTAGTTCTTTAATTTGAGCTGGTGTAATTACAGGAATTGTATTACGTTTCGCAAATCAGCATTCCAATCAAATTTTTAGTAAATACTATGAATATACAAAAGATGGTAATTTAATTATTAAGGTTCGTTTATTTACAAGAGATAATGATATTAACCAAAAGATTTATGCGATTTTTAAAGATAAATTAGGCAAGGAAACTTCAGTTTTAATTACAAGAAATAAAGAAGTAGCACGAATTGACACAACGAGTTTAGGTTTGAATAATTCTTGAGTTTTAGATCGTATTGTTGATGAAAATAATAATACATTAATCAATAATAATGAGTTGGTATGGCAACAAAAAATACTTGTTGATCGTCCTAATGTCTTTAAAGTTAATTTTAATAAAGAAGGCGATAAATTTTTTGAAATTAAATTAAACCATGAATTGAGTAATCAGTATTTGACTGCGCATTTTATTAATCAAAATAATGCTAAATATATTCAAAATATTCAGGTTGATCATAATGGTAATGTAATTGTTGGAACTAAACAACTACCTTTAGGAAATTTTTATAAATTCGTAGGTTTAACTTTAAAAGAAGATAATAAAGACACAAAAATTGTTAATTTAGATGAAATTAGTGAACCCATTCGTGGTATTGATAAAACACGCAGTTTTGATGATCAAGGAAACGTTATTATTAACGCACGTGTTTCACAAAATTTAGCTAATCAAAATATTAATGCCTTATTTGAAGATGAAAAACACCAATTACACACTTTAAAGGCAATAGTTGGTACTGATGGTCTTGTGCGTTTATTAACAAAAGATTTAGAAAATCACCATGATTATAAACTAATTCAAATTGTTGATAAGGATAATCATGATGTTATAAAACCTAATAAATTAGATGCGGCACATCAAAATATTGTTCATAAACCAATAAAAATAAAAATTGGAACTGATAAAAATGGTTTACAAAAAGCACTTCATATTCAAGTTGATTCAAGTTTAAATGATCGATTATTAACACTTAGTTTTGAAAACGAAAACCACGATAAAACCAATATTATTGCTAAGGTTAATAATGGTGAAATTACTGTAGATTTAAACAATTTAAAAGATGGACACTTATATAAACTAGATAATATTAGCATTAATAATACCTCTCCAGCAAAACGCGTTGCTAACTTAGATGATTTAAATGAAAGTGCTAAAACTATTAATAAATTAAACACTAATGCATTAAATGATCCATATCATTATGATGATAAAGGGAATTTAATTTTTAATGCTAAAGTAGATTCTAAACAATTCGCTAATGATCAAGTTGAAGCCGTGTTTATTGATGAAAAAAATAATGAACATAAAGTGTTGACAAAGGTTGATCTAAATGGTAATATTGTAATTAACACTTCTGATTTAAACTTAAATCATACATATAAACTAAAACATGTTATTAGTTTAGAAGAGCCAGAAACAAATGTTTTAAATGATTATGAACTTGCAATTAATCAAAAAACAAAGTTTAGTAAACCAGCTGCGATTGTTGGGACATTAGAAGATAATAATTATAAAATTCAACTTTTAAACCCTGATTTAGCTAATAAGTCTGTTGTCTTAACTTTTGAAGATGAAAATCATCATCCTTATCAATTAACTGGTAAATTAAATGATCAACAAGAATTAATTTTAAGAACAAATGATGATCATTTACTACCTAATAATCACACTTATACATTGACTAAAATTACTAATGTTAATAATCAACCAATCGTTAATGTTAATGATTTAGATAGAAAAGATAGATTATTAGAAAAAGTCGATGTAAATAAATTACAACAATTAAATACATACACCTATGATGATTTAGGAAATTTAATTATTGACATTAATGATAATTTAAATAAAATAACAACTCAAGATGCTGTTGCTCATTTTATTGATGAAAATAATAAAGATTATTTTGTTCCTACTAAAGTTGATTTAAATAAGAATAAAATAAGTATTAACACAAAAGATTTAGATGCAAATCACCACTATCAATTGTTAAATATTGTTGATAAAAACAAGCATGAGCAAATTTTATTAAATTCAAACGAAATTAATCCTTTAAACAGAATTAGCATTATTAAACCACTTGCAATTATTAAACAAGATGCAAATAAAAATTTAAACCTACTAACTTTTGATAAGAATTTGTTTAATCGTTCATTAGATGCAACTTTTATTGATGAAAATCAAAAAGAATATCATGTTAAAGCAAAAACTATTATTGATTCATTAGGTCAAACACGATTTATAACTAGTGATGAGGTTTTACCTAACCATCATAAATATCAACTAATTTCTCTTAAATATGATGGTCAAAAAGTAATTAATGTTGCTGATTTGGATCAAAAACAAATTATTGTTAACAAACTAAATATTGGTAAAATTGTTAATAACCAATTAGTTTTTGATGTGCCAAAAAATGTTGAAGAAGGCGAAGATGTTTTTGTTAATTACCAAGATGTTGATGGTAATTTGATAAAAATTCCTGCAATTGTTAATAATAAACACCAACTACAAATTGATCTATCAAACAACCCTAATTTTACAAAAGATAAGATTTATCATTTTGATTCAATTAGCATAGAAGATGAATATCACATTCCTAAAGAAACAATTATTAGTTCAGATGATGTTGATAATTCAGTTAAAACATTAAATAATGAAGTTGATCTGGGCCGTAAAATTATTGTTAAAAATCCTAGTGTTATTCAAAACAAACCCACAACTTTAATTTTAACAGTTAATGACCCTAATAAATTAGTTGCAAATGAACCATTAAAATTAACACTTGCTTTAAAAAACGATCCAAAACATTGTGTTAGTGCTTATGGCTTAATTGATTATAAAAATAATAGTTTAGTTTTTGATTTTAATCATTTACAAACAGATACTGCTTATTACATTAAAAATTTAGTTTTTAAAAATAATAATTTAAATCTTACTGATTTAAACGCTGAAGCTCATAGTCAAACTAAAATCAATATTATTAAAGATCAAGTTATTGGTTATACATTTAGAATTTTTTCTACTAATACATTGGTATTAGAGGATTATCATCAATATGATACTGATCCTAATGCAACTAAATTAAATTTAAATATTAGTACAACTAATAAGCACCCCAAAACAATTACTTATCGTTTATTAGATGAAAATAATAACGCATTAGAACCAGATTTAGTTGCTAACCTAAATTATGATTCAGCTAAAAAAATTTATGTAACACATTTACAAAATTTAAAAGGTAATAGTAAATATTTATTGTTGGGTTTTAATGATGAAAACAACAATAAAATTCATTACTCAACTCCAATTAAAACTCCATTAATTTTTGGCACAACTCCACATGCTACTACAATTAATTTTGATGTTAACAATCCTAAACAAATTATTAAATCACAAGATGCACGAATAGCAATTGATTTTGTAGATCAAGATCAAAAATTAAGCATTGGTAACCCAGTTGAATTAACTTATTTAAATAAGAATAATCAACAAGTTTATAAAACAATAGGGTATGTTAAAGGAATCAACAAAACTAACAAAAGTGGTACGTTAGAATTTGAACTTAATAATTTAGTATCTAATAATAATTATGAATTGTTAGAATTAAAGGCCTTGGGTTTAGCAAATGATGCTTATCAAAAGGTTGTATTTGCTCCTTTTGATCAAGAACATAATCAATTTAGTACGAAATTAATTGGAACATATATTGAATCAATTAGTTCAAATGAAGTAATTAAAAAAGATGAAAATAAACTTGATGTTAATGTAAAAATTGCTTTCTCACAAAAAGATAAAACATTAAATAACGTTTGAGCACAATTAGTTTATATTAGCAAAGATAATGAAGTTTGTTTATCTCAACCTTTATTATTAAATGTTGGTAATAGTAACTATAAATTCACATTAACAAATAATAAACCAAATGGTTTTTTATCAAACCGTGTTTATAAGTTTGTTAAATTAATATATGCACAAGATCAAAATGATTTACAAAGCAGTGATGCAGTGATAAAAAATGGCACTGATTTAAGTCATGGTTTTAAAACAGAACCAACAGGAAGTATTAGTTTAAAAAATAATAGTTTACAAGTAATTAAACGTACTAATGATAGTATTAGATTGTCAATTCCATTAGTTGATAATGATCATGTCTTAGTTGATAGCGATGGTAGTTTATTTACTATTAAAGTTAGTGATTTAGATGATCCTAATAATCTTTTTGATGCAGATAATCAAAAAATAGTGATTGATCAAAACGGAAATCGTACTTTAGAGTTTGATGTTCCTAATGTTTTATTAAATCATAAATATCATATTAACAATATCACATTGACTAATAAACCAATTAATGCTGCTTTTAATCAAAATAAAAATCATGAACAAGTTTATGAGCTAAATAATTCTCAACCACAATTAAGTGAATTCGGTAATGAATTATTAGTTAATACTTATAATTATGAGCCAAATATAACAGATGCAAAAACAAATGTTTTAAATTATAAAGTTAAATTAACCCTTAAACGTTCATTGAATTTAATGAATGAATACTATTTAAAACTAAAGTATATTGATAATAATGGTGATGTTGTTTACTCTGATCCTGTTTCAATCAATAATGCTAATAATGAATATGAATTTAATTTACCGGACAAAAACGCATTAAAATCAAACCGTATTTATAAATTTGATGGGTTATATTATTTTAAAGATCTTAATAATGTTAAGATAACATCAACTAATAAGGTTAATATGAACAATATAACACCTTTACAAATTCAAACAGCTTCAAAAATCAATCTTGATTCAGCAAAAGTAACTATTAATAACATCACCCCTGCTAGTGCAGATTTATACTTACCATTAGTATCAACAGATGATATTTTTGTAAAAGATCAAGTTATTGATGTTACTATTGGTAATAAAGATAATGCGAAAAATGATTTAATGTTTACATCTAATTTAGAATATGATAATATAACAAAAACATGAGGCATTAAAATTCATGCAAATAATTTAAGTCCAGAAGTTAATTATCAGATCAAATCTGTTAAATTTAGACAAAAACCAGTATTAGGTGCTTTTAATGTTAATAATAGTGATGATAATCATGTATTAATTGATTCACAAAAAACACCAGGTTTTAGCACTCCAAAAGCAAGTTTTGATTTAGTTAGTGTTACTGCAGGTGATGTAGTAGATGCGAATGTAAAAACTAATAGTGTTCATATTGTTATTAATAATGATGGAACAAGTTTAAACAATTGTAAAGCTAAAATTGTCTATAACGATGGCGAACATGATTTAGTATCTAATAACTTTGTTGTTTTAAATTATGGTGTTAATGAATATGATTTTAATTTAGATGATTTAAAACATAATCGTATTTATTCATTTAAAAAACTGATTTATACTGAACCTAATCAAAACGATTATAAATTTAGTTTTTTAAATAATCAAAAAATTACAAACACTTTTAAAACAGCATCAGTTAGTGAACAATTAACAATAGATACTAAACTAGTTCGAAAACCAGATTCAAATTTAAATAAAATTAATTTAAAATTACAAATAAATGATCCTAATGATTTTTTAGAACAAGATAGTGTTTTAGAAATTACATTCCATGATGAAAAAGATAAAACAACTCATAATGTTATTGGTAAAATCAATGTTGACGCTAATAATAATAAAACACTTGAATTTAGTGTAGAAAACACTAACACATTCAAAATTCAACCAAATCATAAATACATTGTTGATAATATTAATTATGCAACAAAAAATAAAATTCAACCTGCTAATGCTATTAGTAATAATCCTAATAAAAACAGTATTTATGATGCTAGTTCAAATCCAAGTAAAATTCTAAGTTTTACAAACGAATTATTTGTTGACAACATTAATGTTAATCAACCTAATACAAATTTAAATCCTACTGCAAGCATTGATGTTGAATTAAAATCTAGTCAAAACTTATTAAAAGACCAGTATTTACGAGCTTTATATATTGATAATAACCACCAAAAAATTTGAAGTGACTATGCTAGTGTAAATAATGCTAATTTAGCACATATTAATTTAAATTTCAATCATTTAACACCTAATCGTAAATACAATTTTGCAGGAATTTATTATTTTAATGATCAAAATCAAAATAATGATGAAACCCAAGGTAAAAAGATTTTTGTAAAAGAGCATTCAAAAACTTATGATTTTAGTACTTTAGCTTCAACTACTCAACTATTAGAATTTAAAACACATAATGTTGATGAAGACCAATTTAATTATGATTTTAGCATTAATAATGATGATCAAGTTTTAGAACCTGGAATGCTTGTTGAATTATGTTTTGAAAATTTAACAGATAAAAACCAACCGGTTTTAAAAACACAAACAGCATTAGTTAAAAAAGATGAACATAATTTTGGCGCTTCAGGGGTAATTACTAATTTATTAGCGGATCATACTTATCGTTTAACAAGTGTTAGTTTAAAACAAAAACCACAATTAGCTAATGTTAATATTAATAACAACAATAATAATGAAATTCTTTTAAACAATATTGAAGATAATCAAAAAACTATTCATACATTATCAAAATCAATTGTTAATGAAATTAGTAATTTAGCAGATGCTTATCCAAAAAATAATAATGGCATATATGATGTTAAATTCAATATTAATATTATTAAGAATAGCAATAAACTTAATAATAAATATGTTAAAGTAGTATTTGAGGACAATGAACACCAACTAATAAGTACAAACGGTCTTTTAGTTAATAAATTAGACCAAACTAATTTAATTAGTTTACAAAATTTTAGTTTTAGTAATTTAAAACCAAATCACCTTTATCGTTTATTAAAAGTTGTTTATGGTGATGAACAAAATTTTGATGCTATTAATGAACAAAAAAATATTTTAGCTTTAAATCCCTCTTTAGTTAATTCAAGCTTTAGTACTACGCCAGCAAAAATTAAAGTTAGCAAAAACGCTATTGATGTATGACAAAACCAGGCCTTGATTAAATTAATTTTAGATGATAGTGATAATCAACTACATAGCGGTGATGAAATTAATATCAATTATCGTATTAAAGGAACACAAAATATTATTTCAACTCCAGCTACAATTAGTGATCAAGATAAAAGATATATAAGATGCATGGCAACTAATTTAATTGCTGGTCTTGATTATGAAATTGTGAGTGTTACAATTAAGAATCAAAAAACCAAGAATGTTTCTCCTATCATTTTTGAACTACCATCAGGACCAATTGGTTTTTATACTCTTGCCCCTGTTTTTAAAATAACAAGTCTAAGTATGGATCCTGTTTATCATGAAAATAAAAACACAGCAGATTTAAAAATAAATTTACGAATCGAAAACATTGGCGCTTCTTTATTTAATAAAGATATCAAATTTATTTTCAAACGTAAAAATAATGGTAAGCAAATTTCATTTATTCATAAAGTTACATCAGCAAATGACGCAAATTATGAATGAGAATTTAAAGATTTATTACGTAATCGCGAATACACTTTAGAGCGTGTCGTTTATCTTAAAAATAAAGATTTTAATCAAAGCAACGTTAGTGAAAGTGATTATATTGATCTATGAATATATGAAAATCTTAATAAGACTTTTAAACTATTACCAACTAAACCATTAGAGATTATTGGTGCACCAATTAAAGAAATTAGTGATAATGGTGCAAAAGTACAACTAAAATTTGCTATCAATGATTCTGATGATGTTTTAAAAGAAAATCAAACCTTTAAGTTTAATATTCAACCAAATGAAAATAATGGTGCTAATTTAAATGAGATTAGTGAACATGAAGGTAAAGTAGAAATTATTGATGGACAAAAATTCTTTGTTGCTAATCTTAATAACATTAAAGTTAATAAAGAATATAAAGTTTATAAAATCTACTTTGATGAAAACCAAAATGTTGGTAATGGTGTTTATAAAATTAATTTTAAAAAAGATTATAAAGAACCAAACAATGTTGTTTATGATGCAAGTGTTAATACAACACAAACATACGTATTTACAAATAAATTTGCTATTGCAAGTTTTAGCAATAACTTAACTGATGTTGATGTAGCAAACAAACAAAACATTAGTATTAATTTGGATTCACGAGCTGAAACAATTCAAGGATATCATTTTAAAGCAAAATATATTAGTAATGATAATCGTGTTATTTGAACTAATACAATCCCTGCGTCAACTAATATAGGTAATAATAAAAATAATATTGCTTTAAATTTTGAATTAAACCAAAACCAACTTATTTCAAATCGTTTATACACCTTTGCTGCTTTATACTATTCAAAAGATGCTAATGTTGATGAAAATCATGCAAATATGGTGGTTATTAAAAATAATGTAAACCCACAAACAATAAACACTAAACCATCAAGTACGTATGTTGATTTAAAAGCACAAAATGCAGATGAAAATAAAATCACTTTAAGTTTATTGTTACATTCAAATGACCAAATTTTTGAAGATAAGAATAACCATCTAAAAATCGCTAAAATCAGTATTGATGAACTTGATGCACATGATCAAATAATTAATTCAACAACGCATGATTATGATTTAACATTAGAAAAAGAAAATAATGAATGATTATTAAAAACCCAACTGATTAATTTAAAACCTAATACTAAATATCGAGTGAAAAAAGTTTGGTTCACAAGCAAACCAAGTGATACTATCTATAATGGCGTTAATAAGGACAATATAGTTTATCCAATTAATAATCATAATACTAATATTGATTTAAAAACTTTAGAAAAATCAACTTTAAATAGTGTTAAACCAACAAAAACAAACTTTAACAAGAATGAATCAATTAAGTTAATGGTTGGTTTTAATAAAACAGGTTCTAGTTTAGAAAACAAATATGCGAAATTAGTTTATAAAGATAATAATAACCAACAAATTGAATCAGATCCTGTATTGTTGAATTCAGATACAACTACAAAACCATTTAATAAAGAATTTAGTTTTAATAATAATCAAACTAGCTTAGTTGCTAATCGTGATTTTGAATTTGTTAAGTTAATTATTAGTGATACTGCTGATTTTAAAAACGCTAATACACTTGATTTAACACCTAATTTTAATAAAGATGATGCTAAATTTAGTATCGAACCTACACCTATTAGTGTTAATAATGTTATTCAAAGTGGATCAAATACGTATGATGAAATCCATCTATCATTCGATTATGATGATCAAGATCATAATTTAGTTGATAATGACCAAATTACAATTACATATCGCAAAAAAGGTGAACAAGCTTGAACAAGTTCAAAAGCTGGTGAAGTTAGTGTTAAAAATCATAAAATTAATGTTACATTAAAGGATTTAACACCAAATACAACTTATGAAATTGGTGCTATTAGCACAACACATGTTCATTCACCTAATGTAAAAACATCACCAATTCAATATAATGAAACTACTAAAAAACAATTAGAAAAACTAGATTTAACAACTAAGGTTGCTCATAATTTCATTAAAGATTTTAAATTAGCATCAAACTCTAAATTAGTAAATGGAGTTTATGAATGTGCAAAAGACGAAAATGTTAAATTTAGTGTTGGTTTACATAAAGAAGGCCATGAACTTGAAGGATATGAAATTTATGCTAAATTTGTAGATAAAACAAATCCTAACAAACCAGCTATTGAAATTAAAAGCTCTAATAACATTAATTCAAATTCAAATCAAGTTTATGAATTTGAATTGTTAAAATCACAAATTACACCTAATCATCAATATGAGTTGAGTGAAATTTGTGCAATTAAAAACAAGGGTGCAAACAATGAAATTAAAAAAGATTTAAATGAAAATCATCTATCATTAGCTTTAGGGTTAGCGCCAAATAAAATGGATGTGCAAAAACCAACAATTACAATTACAAAAGTTCAAGATAATCAATCTGTTAGTGCACATGTTAAATATATAATTAATGATCCTGATGGTATTTTAAATTCAACTGATTGCTCAAAAGAATTATTAAATGTTAAATATGCTTTATTTAATCAAACGCAAAATGCTAATTTAATTAACAATATTACAATTAAATATGATCAACAAAATAAACAATTCTATTGTGAATTTGATATTGATAATTTAACTTTAAATCAAGATTATGGTATTTTTGAAATTAGTTTTGCTAATAAACCAATTCATGCCGCATTTGCTAAAATTAATGATACCCAACATCCTTTAGCTCTTTTAAGTACTAATAATCAAGATATAAATCAAAAAATTGCTTTAGATTGTGAAGTTAATAAAGTTACAATTAATAAACAAGATCTTGATTATGATCAAAACACCAAGATTAGTTGACCGATTGAATTAAGTTCTAATTTATTAAACAATAAGTACATTCGTGTTAAATATAGTGTAAGTGGTCATAAGTTTAAACAAGATGACCAATTTAGTAGTGCTTTTAAAATTACTAATAATAAAGCTAGTGTGGAGATTGGTCATGACAAATTAATTGCTAATAAGGCTTATGAAATCCAAGCTATTTATTTCTCAGACCAAAATAATGATTTTGCATGCACAAACACAAATCAAATTCCAATTAATTCATCAATTACAAATAAAAAAATAGTTATAAATCCATCAGCAACAACAATTAGTTCACATGAAGTTAAAAATATTACTTCAACATCAGCTGATATTAATTGTTCTATAAGTACAAATGATGGTGATATTTTTAAACCAAATTCACAAATTATAGGTCATTTTGCTAAAGGTGATGATTTAACTAAGACTTTTGTAGCTAATGGTGTTGTTGCGAATGTAAATAACCAAACAAAACTTAGCTTTACGTTAAATAATTTAGAACCAAATACTAAATATACATTAGTTAGTGTTGAATTTAAACAACAACCAAATAATGCTTATAAACATATTAATAACAAATATAATGATCAGTTTTATTCAAATGATCAAACACATTCAATTATTTTTACAACAAGTACACCTATTTTTGAAATTAATAGTATTAAAACTATAAAAAATACTTACGCATCAAATAAAGAAGAAATTAAAGTTAACGCTAAATTTAATAAAATTGATCCATATTTAAATGCTAAAAAACTAAAATTAGTTTATCAATCACAAAATAATAACGAAACCTTTGAAACTCAACCATTATTAGTTAGCTCAAATACACATGATTATGTGTTTAGTTTTGATAAAAACGCTTCAAATTCAATTATTGGTAACCGTGAATATCATTTAGATAAAATTTTATATTTAAATGAACATAACCAATGGTGTGAATTAAATAAGAATAATCTTGTTGACCAATTTAATGTTGAACCTACACCAATTAGTATTGCAAAAATTGTTGAAGATGATTCAAAAAGAACACAAAACGATGTTAAATTTAGAATTTATTTTAATGACTTAAATCAAGATCAAATCTTTAATGATAATGATAATGTTAATGTTAAGTTAGTTTATCCAAATACAAACAATGTATGAGTATCTCATGATTGACCTATTCATTTTGACCAACAAGAAAATAAATGATACACTGAAGTTTGATTAAGTGTTGGTAGTTTAAATAACCAAGAATTTGAAGTTGGGGCTGTATCAGTTAATAAAAAACCAGCAAAAGCGAAATTTGATATTCATAAGACAAATCATGGTTATGATAATTTTATTTATAATAAGGATATTAAGAATCAAAATTATCTTGTAGGAAACATATTTAAAGTTAATAGTATTAATATTACACACCCTTCAACTCCAACTGATAGTAATGTAACAGTTAATGTTAAAATGCAAAATAACATGAAGAGTGCTTTTAAAGGTAAATACTTAGCCTTAAAATATAAACGTAATGACACTAACGAATTAGTTTGATCTTTAACTAAAATTAAAATTGATGAAAATATTCATGACGATTTAGATTTTACATTTACAAATTTAGCTAAAAATCACATATATAGCTATGTTGGTGTTTATTGTGTTGATGATCATAACAAAGAAGTTTTTGTTGCTAATAATGTTACAAACTTAAATTCGCCACAAATAAAAGTTCTAACAAATGCAATCAATACTATAAGTTATATTAATGCAAATGATAAGATTAGTAATCTTACTCATAACACAGCGAAATTAAAATTAACACTTAATGATCCTGACCATATTTTTAAAGTTGGCGATGAATTAGATCTTGTGTTTAATACAAAAATAAATCCAACTACTCCAAAACAAAAAAACATACGTGTTAGTGTTAAAACAATTCAAAATAATGAAGTAATTTTAAATGATGTCGATCTTAGCGGATTAAGTGAAAATACAGAATATGAACTAAAAACGGTTAGTTTAAATCAAGAAAATTGAAGTCAAAAAGAAACTTATATTTCTTCAAAACCTCATTTTGAAATTAACAATGTTCATTTTACAACTATGAAACACTTATTTATTAATAAAATTGATATTAACAAAAAAGCCCAAGAAAACAGTTATACCCTAAAATTAACTTTAGATGGTGATTATTCTGAATTTATTAAACAAGGTTATTTACGTTTATTATTAGTAAATAATAAAAACGAAGAGTTTAATTATACAAAATCACATAAAGATACAGATAAAAATCAAGATGATAATGCTGTTAAATACTTTACTTTTGACCTTAATAATTTAACATGGGGAATGCAATATACAATTAAATGAGTGGGTTTAGCAAAAAATAATGAGAGTGATAATACTGATTTTAAAAAATACAAATTAGAAAATGGTTATCTAACAAATAAAAAAGACGATTTAGATCAATTTACAGTCCGTGCAAGTAAATCTTTAAAAATTAAGCAAGTAAATGTTAGTGAAATCAAAGCACATCATGCAAAACTAAATCTAAAACTTGAAGACCAAGATAATGTTTTAAAAAATCTAAATTATGAGCTTGAATTACGTTTTGGAGCAAAAAATAATCAATCATCTAATTCATGAATTACTAAAAAACTAAAAACTAAAATAGTAAATAATGAAATTGTTTTAGATGAAATTGTTTTTGATAACTTAACTGAAGATATGCAATATGAACTAAAAGATGCTAAATTAATATTTACTGAAGAAGAATTGAAGAAATTTAACAAAGTAACAGCAGTTAATAATTTAAATGTTGATTCTGATCAAGTAATGTTTAAAACAATGAAAAGTGCATATGTTGAAACTGCAACAATTAATTTTGTAAATAATACTCCAAACGGTACAATTGATCTTAAACTAAATGGGGATTTAAGTGTATTTAGTAAAAGTCAATATATTCGTTTTGAGTGAAAATTAAATAATAGTGATCCAAATACTAATCCACTTTATTACACTATTCCAATATCTTCATTAGATCTTAATAATTTAAATTGAAAAAAAGATTTTGGTAGTGATTTATTATGAAATTCATCTTATACATTACGTGTTGGATATGGAGATGATAATAAAGCTACTGATGATAGTAAATGAACATGATTAGCATTAGAAAAAACTGCTAAAGGAAATATTAAAACACCAAATGCTCAACGTATTAGTATTAATCAAATGCAAACTCCAACTAATTATTCACTATTAACACAAGAAGTTAATGAGAATGCAAATAAAGTTAAAAAAGCATTAGGGGCTTTAGACTTATGTTTTGAGTTGAATGATCCAAGTAATATATTAACTAATCAAGATCAATTTAGTATTAAATACGCAATGTTAAACAATCTAAAACAAAGCGTTTCAGCGACTGGACAAGTTGAAATTAAAGAAAATAAAAAAATAGTTAAATTCCATATTAGTGGAATTGATCTAAATCAAGATTATAAAGTTTGCGAGTTAATGTTGTTGACAAAACCAGCTAGAATGGGTGAAAAAATCAATAATAATGATAAAAATAACTATTCATTTATTTATCAAAATTCAACAAACGATGATAATCAATATAAATTTAAAATTTTATTAGAAAATAATGATTTATCAACTAAAGAACGTCCATCAAAAACAGGTGATATTGTTAATAATAAAGGTAAGGTTGATATTACAATTACCCAAGCTCCAAAATTATTAGCTGATAAATGAATTAGTGCAGCATATGTTGATAATAATGGTGAAATAAAATGAACTAAACCTCGTTTAATAAAAACTAATGATAATAATCAAATTCTTTATCAAGCTGAAATTGATAATTTGACTACAAATCGTTATTATAATTTAAATGGTTTATATTTTGCAAATCAACAAAGTGATTTTGAACAAGGTAATGCTACATATTTACTAAGTTTATACCCAGCAAATTTATTAGTGCCAACTGATGGTGTACGAATTAAAAATATTACAACGCCAGCCACAACTGATGCTGGATCAATTTCATTTAAATTAGAATCAAAAGATCAAGTATTTGACAAACAAGTATTACATATTCAAATTACACCATCTAATCAATATATTCCAGTATTTGAACGCACATACACATTAAGTGAAAATTCTAATAGTGCAAATGTATTTTTAACTTTACACAATTTAGAGCCTAATACTGAATATAAAATTACTAAAATCTGATTTGAACAAAAACCAGCTAAGGCTTATGCTAATTTAAATTCAAAAGCTAATAATAATGTAATTTATGAAAATACTAATGGTAAAACACAACCTAATATTTCATTAACAACTAAAACCGTTGAGCCAAAAATCATTAGTTTAGTTAAAAAAGATCAAACAAATGATGGTATAAGCTTTGATTTAAAATTGCAACATGATGGAACTTATATTGGTAAACAATTCCGATTGGTTTATGAATCTTCAGAAAGAAAAGGTAAAAAAGAATGGGTTGAAAAGAATAATAATTGAAAATTTAAAAATAAAAAAGAAATATGTTCTGAATGAATAAATTTTAAAGCAGGTCAAAAAGATTATTTAGTTAAAATTATTAAAGATCATAATCAAGATTTTAAGGGTAATCGTGAATATAAGATATTAAGGATTGAATGAGAAGATCCTCATGTTTCTCAAGATAAACACGGTAGAAAAATATTAATATCTAAAGATACTGATAGTGAAGCATTTAAAAATGTAAATAACAATGCACTATCATTTAGCACTGAGGCAACAACAATTAAATGATATGTTGCAACCCCTAAGATTATTTATGATAAAAATGATGAAAAAACGACATTTCAAATTCGATATGACGATCCAGATGACAGTTTATTCAATGGACAAAAAATAGGTATAAAAACATTTTTAGACCTAAATAAAAGTAAACAAATATTTTTAAATTATCCATTAGTTGGTAAGGTTAATAGAAGTAAAAAAATCATTGAATTTGATATTTATAATAATGACTATTTTAAACCCGGTAATCTATATCAACTTATTAATATTGATTTAGTTTATGAACCTGATGCACTATCCCATAAGGATTTAAATAAATTAATGTATGAAAATCCACAAACAAAAATGTATAAAGATAAAGTAACTTTTGAATTAGATAATAGTAAATATGCAAGTATTAAAATTGAAGAAAACCAAAATCCTTATGAAGTGAAAAAAGTGAAAAATAAATTAGATAATGAAAATTTAACACTAAAATCAACTGTGATATTTAACAATAAAAATGAACATCTTTATAATAAATACATGTTTGCTTTATTTACTAATACTAAAACAAATGCAGAAGTTTGATCTAAACCAATTCAATTAAATGCTAATAATTATTCACAATTAGAATTTAATTTACCTAATAACGGAGCTTTAGAAGAAAATAGTGAATATCAATTTTTAGGTGTTTATTATAGTGAAAATCAAACTCCTCCAAATAAAAATAATATTCAAAAGCAAATTTATTTAAAAAGTAAATCAATAATAGAAAATATTAAAACAGGTATTCATATTGATGTTAATGAAGTACAAAATCTTGCGCAATTTAAAGACGGAATTTCAAAACAATTTAAATTAGTTGGAAAAAATAATAGTACAGTTTTAAACCAAATTTTAAATGATAGTTTTTCTATAAATATTAAATACAGACGCAAAGATCAACCTGAAAATGAAGAAAGTTTTAATACTGGAGCATGTAAATTATTTAAGAAAAATAACAATGACCCATTTTGATATTTCAATGCAGATTTTAATAATTTAAATTTTAATCAAGATTATTATATTGAAGAAATTTCATTTGTTGATAAAGATAATAAAATTATTGGATCAATAAATAATTATGATAATCAACTTATTATTTATACAGCTGATGATTATAATTCATCAAAATACCACATTAAAACCGTGGGAGCTCAAATTGCATTTGATCAAACTACAGTTGTTAAAAATGATATTGTCTTTACAAAAGGTAGTTCTGGTGGTTACCCAGCATCAGTGATTAATTTTGAATTAAAAGGTAAAGAAGCAAATACTTTTGTTGAAGGACAAACTTATAATGTAGTTTTATCAACATGAGATGATCCATTTAATACACGTGATTATGAAAATAACATCGATAATCATTTAGATTATGATATTAAAAAAGATTACGTGATTGTTTCAATTGTTGCGCATAATAATCGTTTAGAATTTAAAAATATTCAATTAATTCCAAACCAAAATTATAATATAGTTAAAGTCTTACACTATCAAGCATCAAATACAATTTCAGCTAAAGATATGGGATATAGTTTAAAAAATGCATTTAGTACCAAATCAGTTGATTGAAAAGTATGAAAATTTGGTCATGGAAGAAATAACTTTACTTGAAAATATCAAGTTGCTTTATCCCTTAAAGGACCATTTACAGGTTTTGGTGGTTATCGTGAAACAATCTTTGATTTAGGATTAGCTAAATCACTTGGTGTTTATGCTCGAATTTATCAACATAATACAAATAATTTAGCTTTTAAAGGCTATGATATTGATGGTATTAATGAAGGTGAAGATACTTCATTACTAACAGATTTTGAAAGAGCTTCACGACCAACAAGTGCTAACGTGTTATCAAGCAGTTGTACATTAGCAAATTACCTTCGAAGAAATTCAGATAGTTTAAAAAATACAATTGATATTGGTAAAGTGTGATATGAACCACGAATTAATCCAAATCTTCCAAATAGTTCAAACGCTACATATTATTCAGGAGAACCAAAAGCATCAATCTTTAAAATTGATGATCAACACAAACACTACACTGATAGTTTTGGTACAACGAATAATGATGCTATGGATACAAATAACGAAAACCAATTTTAATTACATTTAAACTAATTGATTAATAATAAAAAAAGCTTTCTAGTAAACAAACATTACTAAAAAGCTTTTTTTTGCGCATTCATTATTGATTTTTATGAAATAAAGAAGCATTGATTAAATTGTCTAATAAAATTTTTTCATCATTATATTGCTTTATTAATTTTTCATTATTTATGATTTGTTCTTGGTTATTATTCATATTTAAATTTAGATTGATGAGTTTTAAACGTTTAATTTGGTTGTTTAATCATTCATATATAAAATTATTTTTAGCTAGTTCAAATGCTACTATATTATCAAACTTAGGGTTATATTTAATAATCTGCACCATTTTTGTAAGTTGGTTAATAACATTAGTATATTTACTGTAGTTTTTATTAATAAATTCAATAAAAGAATCAGTGTTTTTTAAAATATTATTAATCTTAAAATGGTACAAATAAAAGTCTTCTATAATATTAATCACTAAACTTTGCTCTTTAGTTAACCGGTTACGAACTTGCGTATCAAGACGGTTTAAGAAAACCCGATTCATTAATATAATTACTACTAAGTGGTTAAAATGTTTATCAATTTTTGTTAGGATAACTTTTGGGTTTGAAGTAGTAAGTTTATTAGTTACTTGTGTTTGTTTTTGATATGATTGAATGTTCTTTTTGTTTTGAATAGAATTGCATTGTTTGTTAATTAATAATTCTTCAATAACTTTTTTTAATAAATTTTCTTCAATTGAAAATGTTTGTTTCATGAAATCAACATATTTATCTACATAAAAAATATCACCATATTTTTTAATGATTGTAAAACACTCTTGTTTATAACTGTGTTTTAGTGAGGGGGTAGATTTGTTTGTATTACTAATTTCTAATAATAAAAATTCACTAATATGAAGTTGTTGGTCTAATAACTTACTTGTTGCATTATAACCGTGCTTATTTGCATATTCATCCATGTCTTTACAATCATTATCTTTATGATTAACAATAAAAATGTTGAATGGGGAATTAATTAATTTCATAATTTGTTTTTTAGTAGCTAAAAAACCTGCTTGATCGTTATCAAAACATAAAATTAAATCTTTAATTCCATAGCTTTTAATTAATTCTAAATGTTTATTAGTTAATTCAACACCCATTGTTGCTACAACGTTTGGTTTGTTAGATTTATGAATTGTAATGACATCCATAAAACCTTCAACTATAAATAATTGATGATTATTTTCAATGGTGATAACATTATGAAAATTATAAAGAACTTCATCTTTTTTAAATACAGAAGTGGTTTTAGTATTTAAATATTTTGGTGTTTTCTCATTTGTAATTATACGTCCGCTAAAACCAACAACGTTATTGAGATGATCATAAATACTAAAAATAATACGATCATTAAAAAAATCATGGTAATTACCATTCTTATCAATTACCAAAAAGGAATTTTCTATTAATTTATTTAATTCAAACTCGTCATTTTCTTGTATCAGATTATTATAATTAGATTTTATTAAATTTAATAATTCATCATTATTTGGAGCATAACCGATTTTAAAATATGCTAAATCATTTTTATTTAAATTTCGTTTAGTTAAATAATCAATAACATGAATGTTATTTTTATTATTTAAAAAATTAATAAATCATTTTTGAATTTGTTTGTTTAAATCAAAAACCTTATTTTTTTTAATTTCTTGTGGCGAAATTTTTTGTAAATGATTCAATAAATTTAAATCTAAATTCATTAATTGAACAATCTCTTGTGCTGCTAAAGTATAAGAGATCTTTTTATATTCAGCAACAAATTTAATCACATCGCCAGCATGATTACAAGCAAAACATTTAAAGATTTGTTTTTTATCATTAATTGATAATGATTCATTTGTATCATTGTGAAATGGACAAATAGCTCAATAATTATTACCCTTTTTAATTACTTTAATGTAATTTTGAATAACATCAGAAATATTTACAGCATTACGAATTGTTAAAAAAACATTATTAGTTATTTTTGACATTGCTTTTGAAAGTTTTCATGAGCTTTTTGGCTTAAATATAAAGATAATTCTGATAATTTAATTCTTTTTTGAGCCATTGTGTTTCGTTCACGAATAGTAAAAGTAGGGTCATGTTCATCACCAAATGAATCATAATCAATAGTTATACAATAAATTGTTCCTATAGCATCTTGACGGCGGTAACGTTTTCCTATACTTCCGCTATTATCAAAAGTTACTGAAACATTTAAATCCAAAATTTTATTATAAACTGCTTCTGCTTTTTCTTTTAACTTATTAACTAACGGCATTACAGCAATTTTATAAGGGGCTAGATCATATTTTAAATTCATTAAAATTCGTTCTTCATCATTTGCTAGTTTTTCAATAAATGTAGCCTCAGTCAAAATAGCATATAATAATCTTTCTACACCGACTGATGGTTCAATAACATGAGGAATATATTTTTGCTTAGTTTCTTCATCAAAATATGTTAAATCTTTTTTTGATAAATTCATATGCACAGATAAATCATAATTAGTTCGGTATGCAATTCCATATAATTCAGAAAAACCATGTAAGAAATTATATTCAAAATCAATTGTTTTTTTTGAATAGTGTGATAATTCTTCTCTGGGATGTTCATGTTTTCTTAAATTATTTAAGTCTAATCCGCAACAAGTAACTAATCAATTTTGAATTTGGTTTAAATATTTATCAAAAACATCATATGCAGTTTCTTCTTTTAAGAAAAATTCAATTTCCATTTGTTCAAATTCACGAGTTCTAAAAATGAAATTACCAGGAGTTATTTCGTTTCTAAATGATTTACCTATTTGTGCAATCCCAAATGGTAAATGTAAACGCATTGATCGCTGAACATTTTTGAAATTAACAAAAATACCTTGAGCGGTTTCTGGTCTTAAATAAACGATGTTTTTAGGATCTTCAATTACTCCTTGGTGTGTTTTAAACATAAGGTTAAAATGACGAATATCAGTTCAATTAAATTGTTTACATGTAGGACAAAAGATTTTATGATCATTTAAAACTTTCATAAATTCTTCATTAGTAGAATTCTCAGCAATATGAATATTTTTGTTAAAAGATTCAATTAATTTGTCAGCACGATATCTTGTTTTACAATTTTTACAATCAATTAATGGGTCTGAAAAATTATCTAAATGCCCACTTGCTTTTCATACTAAAGGGTTTAAAATGATGGCACTATCTAAACCTACAATGTTTTTTTGTCTAGTAACAAATTCCTTCCACCACAAATTTTTTAAATTATTTTTTAATAATACACCTAATGGTCCATAATCTCATGCGTTAGCTAACCCATTATATATTTCTGAATTCGCAAAAACAAAACCAACAGATTTTAAATGATTTACTAATTCTTCTTGTGTTTTAAATTTATATCTCATAATTATTGCCTATTTTTTAAAATCCTTTTCTTTATTTAAATATTTTTTATTATTGTTAAATAAATTCCTAATTGATCATAAATAACAGAGTTTAATATTTTTAATAACCCTTTATATATAATTTCATTATCCTTATCCTCGCCTTTTTTTATCTGTGTTGCAAAATAAAGTTTTTGTCACAATTTTAACGCCTTTGGTTCATAATCTATTTTGTTAACAATCTTAGACTCACAGTTAACACATATAAGACCATGATCAGCCAAAGAAAAAGTTTTAATATTTTTTGAGCTATTACAATGAACACATGCTTGAAAATTAAAATTAACTCCATTCATTATTAAAAATTTTACCAATAAAAAACAACTTATATAATAATCATTATATTCTAAAATGATATATTCTAGTATTAGTTGATAGAATTGGTAGAATTCAACATTAAAACTAACAACTTTAGTCATTAATTCGCTTAATATTAGCATTGAATATGTATTTTCGTACTTATAATTAATTTGATCTAAGGAAATAACTTTTTTTAATTTTGAAAGTTTTGTATCATTTCTTGCATAAAAAAATTGTATTTCTAAAAAATTACCAAAAAACAAAGCTCTTGCGTTTTTAGAAGTAATTCTTTTTGTTCCAGGTGCAAACATAATAAAACGATTGCCATATTCATTAATGAATGTTAATATTTCATCAAAAATATCATAATCTTGGCGTGCAACTAAATATCCTCTAGTTATTATTTCAGCCATTATTTTTTAATTTTATACCCTAATGATTTAATAATGTTGTTATTATCACGTCAATTGTTTTCAACTTTAACAAAAATTGTTAATGAAATATGACAATCATAAATTTCATGCAACTGTTTTCTTGCTCCCATGCTAATTTTTTTAATCATCGTACCATTTTGGCCAATAATAATAGGTTTATGAGAATTTTTTTCAATTATTATTGAAGCTTTAATTATCCAATGATTTGCTTCTTGGTTGTATTTACTTTCATCAATTTCTACACCAACACCGTGAGGAACTTCATGGTTTAAATTTAATAAGCATTGTTCACGAATAATTTCAGTAATAACAAATTTATCTTCTTTTTCGGCTTTTTGACGAAAATAATCTGTTACCACATCTTTGTGAAGGTAATTTTTGATTGTACTAATTAATTTATCAATGTTAATAGCATGTAAAGCACTTATCTGAACAACATCTTTAAATACTAGATGTTTTTTTAAAAAGTGGATTCTCTCATCAATTTGATCCTGATTCTTAGCCATTTCAGCTTTTGAAATGACTAAAATAACATTTTCTTTGTTTGCTTCTTTAATTAAATTAATAATTTCAAAATCATTGGCATCTAATTCCTTGTCCATAGTTGTCACGAATAAGATTATATTTGCTTCTTTATATGAAATTTCAATTTCATGATTTAAAAATAAATCTAGTTTATTTGATGGTTCATGAAACCCCGGTGTATCAGTAAAAATAATTGCTGATTCATCATCTTCATAAATCTCTTTAACAGCATTGCGTGTTGTTTGTGGTTTATTTGAAATGATTGAGACTTTTTTTTTCATAATTGCATTAATTAATGTTGATTTACCAACATTTGGTTTTCCAACAATAGCTACGATTCCGTATTTTTTAATCATTTTTAGCACCATTTATATTTCTTGTAATTTTTAATTCATTTAAAATTTCGTCTTGTAAAGCAAACATGATTTTTTCATCTTCAATTTTCATATGATCATAACCTAATAAATGAAGTAGGCCGTGTGTAAATAAAAAGCATAGCTCACGTTCAAATGAATGAGCAAATTGATTTGCTTGATCAACAACTTTTTGATAGCAAATATACATTTCTCCTAACAAAGGAGTAATAATTTCACCATCATCAAATGCAAAACTAATAACATCTGTAATATAGTCTTTATTACGATAGTTTCTATTAATTTCTTGTATCGTTTTTTCATCAACAAAATGACATTCAAAAAATCGCTTTTTATCAATATTTAATTTCGTGGAAACCAAATCGGTTATTTGTTTAAAACGTTGTGAATATAATTTATGATTAAAGATTTTTTTAACTTCATTCGTTATTAAAAAATGCATAACATCCTTATTAAATTGTTTTTTTCTAATATAATTAAATTATAAATAATTATAACCTTAACATATTACTTCATTGTTTATTTTCCAAAATAATCAAAATACTTTTAGTTATTAATTTATAAACATTCTTTTACTAATAAATTGGTCTAAATAAAAAATATTTAAGGTTAAAACATAATTTTTGTACAAGTTTAAATATAATTAGGAATTTAATAAGTGATATCATGACAATTAATATATAATTATTAATTAGTTAATATGAATGTTTTTATGAGTTTATAGTATTTTTAAAAATATGATTCAAGGTTAGTTGGTTTTACAATGTCATTTAAAACAAAAATAAAATTCAAACAGAAATGAAAAAAAATTTTAGCATTTTTTGGAATTGGTACATTAACAATTGCTGCAATTGTAGGTACTGGTATTGGAGCTGCTAATTATGCTAATAAGAATATAAAAACTGGTGCTTTTGGTGATAAAATATCAGCAAGAACACAAGTTTTATTGAATGATTTTCAAACAGAAGCAGAACAGTTAAATTTATTAAAAACTACAGCGAATCTAAATCAAAAACATTTACAAGCATTAGGTGTAAATAATGTTAGTGCTAAATATGCTATTTATGAACGTATTAATAAGAATACAAAAAAACACGAAAAATTTGGGGAAATTGTTTATGAATTTTATCCCACAAATTCAAAATTAGATATCGTTAATTTTCTAACTAAAACAAAACCATACGAAAAAATTAATTCAAAAATCCAACTTTTAAGCTTATTTACTACGGCTAACCGTTTAGAACTACAAAACATTTCTTCACTTTTTTCACCAAAACCTGAACTTTCACAAAACAGGGATTTTGATAATACAAATTGGTTAACTTTAAATAATGATCCTAAAAAAATCAGTGTTAAAACGACTAATGATGGTCAACAAGTTGAAATTTTATTACCAAAAACTAGTTTTGATCATCAAAAATTTAATTTAGATTTTTTTGCTAAAGAGTTTGATGCTAATTTTAATTTTAATCCAATGGGTAATAAAACAAGAGCTCAAGCTGTAGCGACTTTTGATAGAACAAAACAAAAACGAAAAGCTCCTGTTAATTCTTGATTATTATGAGTTAATCGCGATGGATTAATTGCAAGATTTAATATGTTATTAACATTAGCACATGCTCAAAAACAAAAATATGTTGATCCTAAAAGTTGAGATTATGTTGATACTGTTTATAAAAATTTAAATACAAACAGCGAAGAAAAAGCTTTTGTTGATTGATTAGCAAAACAAGATTTTGATAAATATTTTATTAATACAAACTCTATTATTGAACCAATTAATGTTCAAACAGATCAATTATTGAATATAATTCGCGCTTTTTATCAATCTTCATCACATAAAGTAAAAATTAAAAATGCAAATAATCAAGACGAAACACAAAATTTTAAAGATAATGAAATGTTTTATTCATGAAATATCAATAAATTATCTTTACTAAGAGGTTTTGTTTACGCAATTGATTATAATAATTTTTTTAATTATTTTCTAGCACCAAAAAAAGATGATAAATTAACTCCTGCCCAACAAAGCATTGTTAAATATACGTCTGACAAGCTAGTTTTAAATAAAGATAATACAACTCAATCTTTTATTAATACAGCCCATAATCTAAAACAATACTATTTACCAACTTATTTTGCACAATCAGTTTTTAGTGAAAATGATATTAAAAGAGATTATGCTAAAACCTTTTATGATTTATTAAATAATTTTAGTATAAAACCATCTAATTTAAAACCTGCGGTAATTAAAACAATTGATCCTATGGATGCGGTTTTGATTGGAATTGCAGTTTTAATCTTAATCATTGCCATTATTATTAGTCTTTTATATAAAATTCCAGGTTTAATTTATAGTTTATTAATGGCTTTTAGTTTTGTTATTAGTTTGTTACTAATAAAATCAAGTAATTTAGGGTTTAGTACAGAAACTTATAGTGCATTAATTATTGGTATTTGTTGACCATTATTAAATTTAGTTAATTTCAACAACCATATAAAGCATTTAATTCAACAAAAATTTTCTTACAAAAACGCTTTAAGAATTAGTTTAAATAAAACGATTGTTAATCAAGGAATGTTTTATTTATTAATGATTTTTATTAGTTTAGTTTTTATGTATTTTGGCAAAAATAACATTAGCATTTTTGGTTTTAATTTGATTTTGATAACTTTTTCTTGTTTGTTAATATCTTATATATTATTTGCTGCAATGATGTATATGTTGTGATCACTTACTCATCATCTCCCAAAACTACATTTATGAAGACAATACTTAGTTGCAACTAATGCAATTAACCAAAATCGTTTTGATGATGAATTTGAATGAAACGATCAACAAAAATACCAACGATTGATTTTTAAAATCATGAATCAAAAGGTTTATAGAATTAGCTTTTTAATTTTTATCTTTATTTTAGGTTTTATTGGATTATTTATTTTAGGTTTTATTGTTCCAAACCATAGTTTTAGTTTTGGTTCTGTATATGAGTTAATTATGAAAAGTGACTTAAAAGACTTTAATTCTCATGAGTTCAAAACCATTTTAGATTATAAAATTAATGATGGAGTAGTATCAATGCAATTTGATGCTAAAAAACCAAATGTTTTAAGTGAAATAGATTTGATCAGTTTAAAGCACCGCTTTAGTGATGCAATTGTTTATAGAAGTAGTAAATATCATTTAATTAATAATATTACAAATTCAATTGCTGCGTATTTTATTATCTTTGCTATTATTTTAATTTGATCAAGCATTTGACTAAAACCCCAATCAATAATTCCTATAATAATTAATCTCATTTGTACATCATTAATTAGTTTTGGTATTGCTGGGTTATTACGTTTATTTAATAATCAAATGTCAATCATTGCAATTAACACGAGCTTTGTTTTAATGACTAGTTTTAGTTTACATATTTGTTTGAATTTAAAACAAACTCTGGATTTAATGAAGCATGTAACAAAAAAACAATTACAATTTCAAATTCAAGATTCTTTAATTAAATACTTTAATACTTATAATATAATATACGTTGTGATGTTATTTGCATTATTATGATTAATGATTTTTACACCATCTGTTTTAATTAGTTTTAATGCAATTATTTTATTTAGCTTAATATCTACCCATTATTTATCAATTATAATTATTTATTATTTATGAATGAAAATGGTTTATATTAGTAAAGGTTTATTAGCTAAGAATGATAATGCAAATAATGTTTATGATAAATTTGATGAACAAGAAATTATGAATATAAATAAATTTTAAAAGAAAGATTTATAACGATTATGATCAATATTGATTACATTAAATCAAAAATCAGGGATGTACCAGACTTCCCTAAAAAAGGAATCGTTTTTAAGGATATTACTCCTTTATTTTTAGAACCAAAAATTATTGAAAAGATTGTTGATGATTTTGCTGACTTTGCTAAATCATTAAATATAGATGCTATAATAGGTGCAGAGTCGCGTGGTTTTTTGTTTGCAGCACCATTATCAATCAAATTAAATAAGCCATTTATTTTGGTACGTAAACCTAATAAGTTACCAAATGATGTTTATAGTGCTGAATATACATTAGAATATGGTAGTTCTCGTGTGGAGATGCACAAAGATGCACTAAAACCAAACCAGCGCGTTTTAATTGTCGATGATTTATTAGCAACAGGAGGGACTGTTGCTGCCATTGAAAATCTTGTTCATCAAGCTAAGGGAATTGTAGCAGGGAGCGTTTATTTAATTCGCTTAGGATTTCTAAAAGGTGAAGAAAAACTAAGTGGAAAAGTTCATGCTTTAATTAATTATTAATTTTTATAAAAAATTAAATTCTTACTTTAAGAACCTACCAACGTTCTTAAAGTATTTTTTATATTGTAATAATATAGGAAATTTACACAATATAATGTTCCAAAAATTATTTTTTAATTTAACCAAAATAGCATTAATTTTTATTTAAATTTAATTTAATTACAATCGGTATAAAATATAAATATTTATTTGTTTAGTGGATTGAATAAATTATGAGATTAAAAAATAAAAATAAATGCTGAAGCTTACTCATTGCTTCATTAATATCAATACCTATTATTAGCACCATTTCTGCATGTAGTTTTAATCAAATTAAATATAAATTAAAAGTTTTAGATTATTTTTTAAAAACAGAAAATGATGATTATTATATTGCTTATGAATTTAATAAATTAACGAATGAAGATATACAAAAAATATCAAAAGTTATTTTTTCAACAAGTATTATTAATAGTTTAAACCAAAAAATTGCTTTTTATGTTGATATAAAACCGATTATTGTAAATAATCGTATTTATATTCATTTACCCCAACGTCCTAAACCTAATGAAAAAATTATTATAAATTCAAGTCAAGAATTTGTTGGTGTTCAAGTGATTCAAACTAATAAAATGTTAACTAAATCCATTAATTTTAATAAAGAAGATCATTTAATTAAAGTCGTTGATCACAGCGCACAATTTACTAAAATTAAAAAAACAGAAGTTGAATTTGAAATTAAGCTTTTAGATCTAAATCTTAATGATATTAAAAATAAAAATATTGAAATAGAATTAACAAATAAAAAAACAAATAAAACCTTTAAATTGCATTTTCTAAAATATCAATTTAATCACTTAACACAACAAAATAAAAAACCAGTATTAACTATTACTGCTAATTTAAATGCTGATGCAAATAACCAATTAGATGATGGCGAATATTTTATTTCTAAATTAGTTTTAGACCAAAAAAAACTTGAAATTAACCAAAGCGTTTATAAACCAAGTGGGTTACAGTTAGATGGAAGTATTAATAATCAAAGACCAACTTATTTATTAATTCCATCTGTTTTTTCAAAATGAACAAAAATAAATGTTTTAGATACTAAAGTGATTAAAAATGATGGAATTCCACAACAATTACAACTTTCATATGATCATTTTATTAACCCATATAATTTTAAAGATAATATGTTAAGTGCAGATATTGAACTTATATCAACTAAAAATAATAAAACTATTATTTTAAAATCATTAAAGTTTAATCACGATAAAAACAATATTGTTTTTGATTTAGTCAATAATTTAGAATTACAAACTCTTATTTTAAAAACTCCTTTTTTTAAAATTAAATCATTAACAATAATGAATGATAAGCAAACTTTGCCATTAAGTATTAGAGAAAAACAGCTTGATTTAACTAATAAACATCTTTCTAAAATCATTAAATTTACATTTGATGATACTGATCCACAAAATATGCTTATGAAATTTCAATTAGATAGTGATTCGCCAATAGTGCAAAATACTAAAAAAGTTTTATTAACAATTAAAAAAATAAATACAGATCAGCAAGAAGATATAAAGACCTTTAGTTTTGATATTTTAGAAAACAAAATAACAACAGGTTCTTTTTATGATGTAAAAAATAAGTATTTTCTATTAAATCTAAATAAAACTTTGAATGCTAAATCGATAGGTCAAATTCTTGAAAATGATTCTAATTATATAATTACTAAATTAACTGTGGTTAATAATGAAGCATCAATCAATGAAATACCAATTGAAATTGATGATTTACAAAATAAACAATTTACGTTAAAATTTGCTGAATACCAATCAAATGTAATTAAATTTAATGCTAAGAATGATCCTGAATTACATCTTAATATCAAACATTTTAAACAACTTAATCTTACAAAAATTAATAAAATTAAATTGACTTTAAAAGACGGTAAGCAAATTATTTTTAATCGTTTAAATAATGAATTTAGTTTCCAACAAGCAGGATTTAAATTAAAAATTAACCAAAACACATTTCCAAATTATTTAAAACAACATCAATATGTAATTAAAAGTATTAGTTTAATAAACGATGATCAGTCTGTGATTGAGTTATTATCAAAACACAATCATGAAGCTATTTTTGAGATTCCTATTACAGCATCTCTTGATACTTGAGAATATGACAAAATCCATAATATATTAAAAATTAAAATGAAATTAGATAGTTTAAGTTTACATCAAAGCTTAAATCCATCAGATTATGCTTTAAATTTTTATAATCTTGATACTAATATTATTAACCGAAAATTTACACTTAGTCCCCAAACTGTTTTATCTTCAAAAGTTAATAATTCAAATGTAATTGAATTAATTTATGATTTAGATAATGATGAAAATATTTATGGAGGTTTAGAACCTGAGAGCACTTATCAAATTAGTTCGTTAATGATAAATGCTAAACCAGTGATTATTAATACTTCTATAACAATGGTTCAAACTAACGCTCCAGCTTCTAAGGTAGAAAAAATTGTTGCTAGTTATGATTCAGTAACCAAAAAAACAAAAATATCATTGCAGTTTTCTAATAATTGATGATATAACAAAAATGCGGATCATAAACTGTTTGCCATTATTTCGTCTTTAAAGCCTAAAAATAATAAAAATGCTAATTGAACATTTTCATTATCTAGAACGTTTAGCAATCCTGAAGCAATTAATTCAAACAAGGACAAAGATTTTGTTTTAGATAAAGCAACTAAAACTTTAACATATGTAATAAATTATGCAAATATTGACTATAAAGATCAAAATGATCATGAGTCATTTGATTGGTATTATGATGATTTAGTACTTAATAAAGTAACAATCATTTATAATGATTTAACAACAAATGATATTAGTCCTTTTTCTAAAGATGATCAAAATTTAACTATTAAACTACCAAACAAAATTAATTCTTAACGATAAAATTTTTTTAAAAAAACTTATTTTTTATAAATAAATAAATGAATTCTATATTATTCATTTGCTTTGCCTTGTTTAAAAGATTAAAAAAGGATTAAATTATTGTTTAAAATTTAATAACTATGAACAAAATTAAGGAAATTTATGAAGAATAATAAAAGCCGGAAATGAGTAATTACTATTTCGTTAACGTTGTTAATCATTTTAATAGCAAGTGCAGTTATTGGCGTTGTTTTTTATTTAAAAAATAAAAAAAAGAATGATACAAGTGTAAATAATGATAGTTATATAATTCAAAATAATAATAATTCTTTAAAAGAACAACCTAACTCAACAAGATCATTTTTAAACCAAAAAACAATTTGTGTAGGTCATTGAAATGTTTTAAATCAAGGTGGAATAAACCAGTTAAAAAATGAAGCTTTAGCAAAGGTGATTCTAAAAAGTAATGTTGATGTAATTGCTTTAACAGAAATTAATAGTGCAAAAAATAATGAACACGATGATTTACCAATGCGTGCCATCTTAAAAGAATTAAATTTACACAAAAATCAATTTGATCAAACAGCTAATTGAAATTATGTGCTATCAAAAAACTTATTTTCAAAAGGCAATGAATCACAAACTGAACGAATTGGCTTCTTTTATAAAGCTAATATAATAAAACCAGTTCAAGAATATATTTACAAAAACAATGATAATAAAGATATTTTGTTTGAACAATATTTTAAGAACTGGAATGATAATACTAAAATTACTTATTCACGTCCGCCTTATGCCTATGAATTTCAAACTTTAGATAACAAATTTAATTTTACAATTGTCGCTAGTCATTTAGATTCACCAGGAGCAAACGAACATAAAACTAGCAAACATGATCATACTTATGGTAATAAAAATTTAAAAATTAATATTAAACAAGGCTCAAAGGAATTAAATGAGGCATATCAATTAGGAAATGTGATGGACTATATTGATAGATTAGATGGAAATAATGATGATTTAATTTTTGTTGGTGATACTAATATTAAAACAAAAAACGAATTAGGAGCCTTTGCTTCACTTTATAAACGTAATTATTTATCCAATTTTAAGGATGATAATGAAAATAACAAAACTACATTAGCAAAAACTTATTTAAAATACGCACAACACTATGACAAAATTTTTACTTCACCATCAATTAAAGTTTTAAATACTTATAAGTATGATTTATGATCAGTTTCTTTAACAAATACATTATTAGATAAAAACTGAAAATTAGCTTTTTTAAAAACCTATTTTCGTTCTTTAAGTAATCAACAAATGCAAAATATTGTTGATAATTGGGATGAAATAGCAAAATCAAATGATGTATACATTAAAAAAGCTACTTCTATTATCTCAGATCATACTTTAGTTGCTGTTAATATTAAAATCGATGAAAATGATATTATTGATGAAAATTAATAAAGAGATTTAAATACATAAATTTATAAAAAAAGAGCACAAAAGTTATTATACTTAACATACAAGTACCTTTTTAAATTTATCGTAGATTAACAATAAAGTTTTAAAAATAGATTATTTATTGTATGATATAAAAGCTCAATTTGCTTTTTAATAGATAATTTCAAAAAAGGATTTATAAAAGATGATAACTAAAAAACATTTAACTAAAATAATTACAATTTTAACTTGTAGTACTTTAATTATTGGTTTATCTTGCTTAATTGGTGCATGCACTAAATCAAAAAGTAAGGTTGATGATAAACAAGTGCTTTTTAAAATAAAAAAATTTAAAACAACTACTAATAGTATTGAGTTATTTTTAGAAGGTAGTGCAGTTTTAGCATCACATTCATACAGTGTTGTTTTAAAAGAAAAAGCAACTGGTGATTTAAAAACAATTAATAATTTAAAATTAGTTAAGGATAATGATCTTAATAAATTAATTATTAATGAATTAAAACCAAATATGGGTTATGAATTAGTAGATTTATTAATTAATAATCAAAAACAGCTAATAACTAAACTAGATTTTATAACAAGTACATTAACACAAGAAAATGTAGATCAATTTAGTATAAAAAAAATTGATTGAAAAAATATTACTAATAATAGTGCTGATTTAAATTTAGAATTTACAAAATATTTTTTAGCGAACGAAAACCAAAATATTTTTGAGATTAGTTTATTAAATTTACAAACCCAACAAATTACAAAATTTAAGTTTAATTATCAACCAAATTCGCCATCGATAACATTTAATTTTACTCAATTAGAAAACAACGCTAAATACCAAATTCATAGGATTGTTTTAAATAATAAAGAGTTAGTTTTTAGTAACCAAAATTTAGTATTATCTAATACTAATAAGCAAAACTCAAACCCGATTATTAAAGATTTAAACATCACCAAAATTGAAAAAGAAATTTATGATACTAGTGCAAAATTGATTTTTTATTTTAAAGATAATAAGTTAGATGATTTAAATAATCAAAAATTTGTTTTAAAATTAGTTTCAGCACACCAACCATCAAATACTTTTAGTTTTGAAAATTATGTTTACAATAACTCTAAAAAGACCTTATCATTTAATTTAACGGGTTTACAAACTAGTACAAAATACTCATTAAATTCATTAACTCTTAATGGACAGCCAATATCATTAGCTAATTTAGATTTAAATATTTTAACCTCTACAAAATTAACTACTGTTCAAAGATTAGAGAACTTAGAACATGAAAGCAGTGATTTTTGATTTAATGATGAAAATTTGAGTGTTAAGATTAAATTATATTTTGAAAATAACGTTAATTATTTAAAAAATAAATATTTAAAATTAGTATACATGGATAATGCAAATAACATCATTGTATCAAAACCTGTTTTATTTGATCCCACAAAAAAAGAGTACGAATTTAGTTTCGATAAAGTTTTATCAAACCGAAAATACACTTTTTCTAAACTAGTTTTTGATGATCTACAAGATTTTAGTGAAAAACAAGCTTTAGAAGTAAATACTCAAGGATTAAATGGTAGTTTTAATACACCTCAAGCAAAAATTAAAATCAAAAAGATTTCTTATAGTTCAACCGATAAATCCGCTAAATTAGAGTTTCATTTAGAAGATGATTTTGATTTAGTTGATGGTGATAAAGTTAGTGTCAAATATAGACTAAAACCATCATCTAATAGTATTGATACTACTAATAATACTGTTGAGGGTTTGGTTCATAACCACATATTGAATGTAACAATTAATGATTTAGAATCAAATAAATCATATGAAGTTATTGACTTTGTTATTAATGATTATAAAAATAAACAATTATTAAACAAACCTGAATTTTTAAAAAATAATACAAATCATCATAACCATCCTTTTAATATCCCGCTTGATTTTGATACTAAAAATAGTGATTATAAAACTAAATTTAATGTAAATGAAATTAGTAAATCTTATAATAAAGAAACTAAATTAACAACAATCAAATTAAAATTTGATAATATTCCCACAAATCCCACTTCATCTAATAATTTTACATTTACAATTAAAAAACGAACAGGATACACTACTAATCTGATAAGCCCAAAAACTTTGGAATATGATGTAAATAAGAAAACTTTAATAGCAAAATTTGAAAATATTGAGGACCAAATTGTTTATGATTTTGTTGATGTTAAAGTAAATAATCAAAATATTGAATTTAACAAATCATTAGATAAGTATTTTTCTATTATTCATAGTGCTGACTATCATGAATAATTTTTATTTTGTCTTATTAATTAAATTTAGCATTTCATAAGCAATTTCGTGCTCTTCATTCACTAAACATAAATATATAGGAATTTGTGATTTAGATGTTGAAATTTTATTTAACGTGTTTGTTAGTATTAAATGATCGTTAATTTCAAGGTTTATTAGATGAATTTTACTAATAATTTTTTCTAAAACAAGATGCTCAAATTCACTAATTCCTCCCGAAAATACAATTGCATCAATCTTTTGTAAATGGTTTAAATACTTAATAAAATAATCAACAATGCTATCAGTGTACATCTCAAGGGCAAGTTCATTATTTTTATTGTGCAAATTATTAGCAACTTCACTAATACTATGATGTTTTGTTAATCCATAAAGACCTGATTGCTGATTTAAAAATAAGATCAATTGCTTCAGTGATTGGTTTTTTACTTTTTTTAAAGTAAGTAAAACACTAGGGTCTATATCACCACTTCTTGTATTCATAATAATTCCTGAAAGAGGAGAGTAAGACATTGAGGTATCAAATGATTTTCCATTTTTAATTGCACAAACTGACGATCCAGAACCTAAGTGTAAAATAATTAAATTAACATCTTGTTTTCCTAAATAATTTTGCATTATTTTATTACTATAACTAAAAGAAATTCCATGAAAACCATATTTACGAATTTTATGTTTTATTGCTATTGTTTGATCAATAGCAATAGTGTATTTATTTTTATTAATACTACGATGAAACCAATTATCAAAAATAATAATAATTTTTGCAGAGGTGTTTAAATGCTTAATGTCATTAATTAACATGATAGTATTTTGGTTGTGAATAGGACATAAATCTGTGTGTTGGTTAAGATCATTATAAATTTCATTATTTAAAAAAGCACTTGCTCCATAAAAATTTTTAACATAAACAGCTCGAATTCCAAAATAAGTGATATCTTCTAATTTTTTAATAATTTTTTTGTTAATTAAAAATTTAATGATTAAATCAACGTGATTGTTGTGATCATCATCAAGTAAATAAACATATTCATTTTTATCAAAGATATATTTTAGTTGGTGTGGGCTAACGTCTAAGCGAATTTTGCCACTAAATATTTGTTTTAAATTCATATCAAACAAGCAAAATTTTCAACTACTACTACCAATATTAATTATTAACATCATAATTAACCACCACTTATTAACTAAACTTTAAAACCAAAAATAAACAATTCTGGCTTTTGATCAGCACTTTATTTATTATTTTACTATATGTGCATATGCTTTTTTAATAATCTAAATTCAATTATTTTTTATTTATTCACTTATTTAAACACCGCTTTTATTAAGGTATTAAAAGCAGTGTTTTTTTTTTTTTTTTTGAATTGATGTTTATGGGTTATAATGATGTCGTATTGTTTTGTGTAAAATTAGTTATTATTAAGGATATAAAATTATGAAATTAATTAAGAAAAAGTTATTATTTTTATTTACAGCTGCTTTAGTTCCTATTGTTGCTACAGCACTTGCATCTTGTAGCAATAAATCAAAGTTAAATGTTAATGTTTTAATGAAAGATATAATTCATACCAAGAGTGACCATTATTATGTTGTTTATGAAGTTAATGAACTAAATACTTCGCAAGGTATTTATAAGAATTTAACTTTAAACACCAATCTTTTTAATCCATTGTTAAATAAAAAAGTTGACCGTTTAGAAAATATTCATCCTTTGATTATTAATAACAAAATTTATTTAAGACTAGCTCGTAAACCAAAAATTAATGATCATTTTATCACTAATTTTTCAAATGATGCATTTCCTGCACTACATTTAATTTTTGATCATAATTTAAATTTTATCGAACAAGATATTAATGAAGATTTAAATTTACAACCATCACTTCCTAATACACCAAAGATTGATCATTTAGAAATTAATAATATTTCGTTTACAAATATTAAACAAAACCAAGTTGATATAAAGATTAAGTTAAAAACAGTTTCTTTTAAAGACGATGCTATTCATAATTTTAGTTTAAAAATTATCAAAAAAACTAATCACCAAATCATTACTAGTGATGCTGTTTTCAATAAGCAAAAAAATGAAATTGATGCTACCGTTTATAATTTAACATCTGATTGTGAATATTTATTAGATAAACTTAATTTTAATAATCAAAAAATTCAATTAACTTCAATTCCAAACCACACTTTTAAAACCCTTGATGATAGTGATAATCTTTTAAAAAATCTAGAAATTACAAAGATTAATGATAATGGTGCTAATTTAACTTTAAATTTTAAAGAGTTAAAAATAAAAGATGATAGTGATGAAGCTTTAAATAATATCCACATTAGTTTAGCATATAATAGAGTTTATAAAAAAGGTCAATTAGAGCTTAATAAGAAGCATTTTTTTATTGATAAATTAAACAAGAGAATTATTGTTTATGTTGATGAATTAGAAAGAGGAACTCATTATGATATTAAACAAATTAAGATTAATGATCAAGTTTTAAATTTAAATAATCAAACACATGATTTTACAACAAAAGGCGATTATGCTAAAGTTGAAGCTATTGATTATGAAACAGCTGTTGATAAAATTACAAAAGCAGTTACAATTAAAGTAAAATTATCTGAAGATCTTGCTTTCTACAATCCTAATATTCATAATTTTAAATTTTTAATTAGAAAAGAAAATGATCAAAACCCAATTGAATTCTCAGAAGTTAAATACGATGAACAAGAACGTTTATTAACTGCTGTAATTAATGGTTTAGATGTTGACAAAACTTATGAAATTGATAATATTTTACTAAACGGTAAACCAGTATCTTTCCCTAATGATGATGACTATGCAGGTGGATATTATGGACCTATTAACAAAACATTTAAAATACGTCCTGATCGATCAAGTATTGAAATTTTTAAAGATAAATCATTCTATAATTCTTACTATAGTGCTTTTAATAGTTGGTTTTGATATTTATCAGTTTCTAAAAAAGATGATAATGAAAGATCAAAAGAAGTAAAAAAAGATTATAAAGAACACCTTTTCATTTCGGATATTACTAAAAATAGTGCGATTATTAATATTAAATGTGAAAAATTACTTTTAAGAAAAGAAAATTCTTTAAAACCCAAAATTAAATTAAGATATCATCCATTAAATAATCCAAATGAATGAGCATATAAGGAGTTTAGTTTTGAAGATAAAAATTGCTTCATGAGTATTGATGAAGATCAAAAAACAATTAAAATTAAATTATTAAATTTAGATAATCATATTAATTATGAATTTGTAAGTTTTATCTTAGAAGATGTTGATGGTAATTACCATAGTGATAATTTTAATGTAAATGTTTATATAGGAGATTTTTTTGATTCTCATCCATTTCACTTGGATCTTTCATTTCTTAAATTTAAAACAAATGAGTTCAAAAAAGCTGTTTTTTCATTCACAAATACAAAATCAACTTCAACCGATCTAAATGTTGATTTACAAGACAATCAATATGCTGATAGTATTGAGCATAAAATAAAATATACTTTAGAAGATGAAAATCATAATAAATATAAGTTTGTTAAAAACTTATCAAAATCAGAAACTACTCAACTTAATGCAACAAAAAACATTATTTTACATCTTTTTGCTTTAGATAAAAATCAAACATTTAAACTTATTTCTTTTGAAATAGATGATGACAATTATTTAACTAATAATATTACAGTTAAAACACTTAATGAATCATCTCCACATTTATTATTAAATTTAGAATTTAATGATATTAAAAATACAAATAGTACTTTAAAATTAACACTAAACCAAGCAGTTATAAATATGCCAAAAAGAGTTGATGTTATTTTAAAAAATAAAAAAACAAACAAGCTGTTGTATTTATTTAAAACAGATTTTTATTTTGATAAAAAAACACACCAAATCTCAACTAAATTAGATAACTTAAACATTAATACTGATTATGAAATTAGTGAACTTTTTATTGATAAGCAACAGATTAATTTAAAAAATATCAATAATAAGACTTTTAAAACTTTAGATTACGGCTTTGATCAATTTTACTTAGATAAAATTGATCATGAAATTAACCAACAACAAAAAAACATTAAATTCAAGTTGCATTTTAGCGATTTCATCTTTAAAGATAAAAACGCCCATAAAATGTCATTAGTGTTAGAAGATGAAAACAACCAACAAGAATGTATTGATTTAAATGCTCATAACCAATTAAAAATCGACTATGAGCACCAAACAATTGAATTTGAATTAAAATATTTAAAGCCTGATATTAAATATCGAATTAATTCAATTCAATTGAATGATAAAAATTTAGATTTAACTTGAGTAGATAACCAACGCTATTTTAATTTTCCTGGAATCTTAAATCCCAATTTAAAATGAAAAGTAGATAATATTAAATATGAAGTTAATGGATCTGATCCAAAAATTAATATTACTTTTGATCAAGATATATTTTTAAGTAATTTATCCCAAGATAAAGAATGTAATATTGAATTAAATTATGTATATAACAATAGCGATGGGGATACTTTAGAGTCAAAAATAGTTGCAAAAATTAATAAAAATAGTAATAAAAGTGTTGAAATTACACTAACTAATGAAAATTGTTATTGATTAAATGATTTAAAAGATATATGCTCAAATCTTGATTCAAATCAGATCTCTACACAATTTTTAAAGAGTATTAAAATTAATGGTCATAAAATTCCTAAAGTAAACTAAGATATGAGAGTATATTTTACACAAAGATTTTACACAAACTAGTTTTTATAAAAATTTAAGTTTTTTCATTTTACTAAAATTTGTATACGTTTTAGTGGGTTTTATAAGTACAAAGGCCAATCAATGGTCTTTTTTTTTTTTTTTTTGCTATCATTTTATGAGCATTTTTAGTTGCAGTTATTAATGCTATAAAATAGTATTTTTTATAGTACATAAAGGAAGCGTTTATGAATAATAAAAAAAGTAAAAAAATCCAAACAATAAAAAACGATAATAATAAAAAAAGTCGTAAGCAACGTCTTTTCTTATTGCTTGCAACGCTAAATTTAGCGTGAGCAGGAGCAATTATTGGGATTGTTTTACATTCAAAAAACAATAATGAATTAAGTAATAATGATTTTTATAGTTTTAATGATGATGGTGACTTAATCATTAAAGGTCAATTACCAAAAACTTATATTAACAAAGATGTTTATGGTGTTTTTGTTGATGAAAACAATCAAGAATATAAAATAAAAACTCAAATTGATTCTTATGCAAAATTTGAATTTGATACTAAGTATTTACCAAAAACAGGTAAATATAAGCTAAATCGAATTATTGATTTAAATAATAACATTGTTCGTGAAAATGAGAAGCTAAAACCTGAACAGAAAATTGAAGTTTTAAAACCAGTTCCAACTAATGTATATGAGGATCAAAAAAACGAAAAGCACATTCAATTAAAGGTAAATAAAATACTTGCTAATAAAATTTTAGTCGCAAAATTTGTAAATCAAAATAATGAAGTTTATAGTATTGAAGCACATGTTGGATTAGATCAAAAAATTGATATTGATACAAGTAGTTTAAAAGATGGCAATGTTTATTATTTAAAAGATCTAATTTTAAAAGATTCAAATCCATTAACAAAAGTTGTAAATGTTAATGATATTGATGAACAGTTTAAAAAAATTGATAAAACACATACTTATGATAATGAAGGTAATCTTGTTTTAAAAGTTGATTTAACAAATAAAACTAAAAACCAAGCCTTGCCAACAGCAGTTTTTAAAGATGAAAATAATAAAGAATATTTAATACCAGCGTTGAGTGTAAAAAATAATGTTGGGTATTTTAATACTAAAAAACTACCTTTAAATCACAAATATGAATTAAACCGAATTGTTAACAATAATGAATTAAATGATGTTTTAGTGCCAAATAATGAATTAATGCTTGAACATAAAGTTAGTGTAAATAAACCAACAAAAGCTAAAGTTAATTTTATTGATAACAAAAAAGTATATGAAGTTGATTTAGGTGTTCAATTAAAAAACACTCCTCTTGAACTTACCTTAGAAGATCTAAATCATCAAACATATAAAATTAATGCAAAAACAGATGAAAAAGGACGAGCTGTTTTTGATATTTCATCTTTAGGTGATAATAATTTATATGAAGTTATTGGTATTAAAAAAACTAATGAAGTTGATGTTGTTAATCTTAAACAGATTCCATACCATAATCGATCAATTAATAATTTAAATTCAAACGCATTAAATACCCCATATCAATATACAAAAAATGGTGATATTAATTTAATTGCTAAAGTAGCGCCATATTATGTTAACCAACAAGTATATGGAATTTTTAAAGATCAAAATAATCAAGAACACCAAATTTTAGCAAAAGTTAAAAAAGATGGAACCATAGCATTTGATACAGGTGCATTAAATAATAACAGTAATTATTCACTAGACAAAATTGTTTCAGTTTCAAACCCACAAAATGTTTTGGTTTCAAATTTTGATTTAACTTCAAAACAAAAACAATTAATTAAAAAGCCAGCTGCTAATGCAAGTGTTGATTCAACTAAAAAAACCCAAATTTTAGAAAATTTAAATAATGATTTAATTAATCAGAAATTAGTAGCAACTTTTGTTGATAATAATGATAAAGAATATAAAGTAGTTGCTAATGTGGATCAAAATAATAAAATTATTTTTGATAGTAATGATTTACCAAAAGGATATATTTATCATTTAGCTAAAGTTGAAAATAACGATTTAAATAAAGTCATTAATCTTAATGATTTTGAACTAAAAGATAAAATAATTGATAAACGAGATTTGAATTTATTAGATTCACATCCAGATTTTACGTACGATAATGATGGAAATTTAGAAATTCACACTCAATTAGCAAATGATTTAAATGATGATTTAAAACAAAAAGCTTTAAATAATGCAAATGTCAAAGGAATTGTTGTTGATCAAGATGGTATAGAACATGAGATAGATGTAAGTATTGATGCTAATGGCAAGGTTATTATTCCAACAAAAAATTTAGCAAATAATGACCCAACTAAACCTAATATATACACACTTAAAAAAGTCGTTTTAAAACAAAATAATCAACCAAATATTGATCTAATTAGTGAAGAGCAATTAAGTGGTGATAATCATATTTCATTTAAAAAACCAACAATAACTGCTAAAACTAAAGAAAATGATGATTATGAAATTAGTTTTTCAAATCCTAGTTTAGCAAATAAAAAAATTAAATTAACATTTAAAACCGATGATAATAATACTAATACAAAAACGGTTGAAGCTAGTATTGGTTTAGATGGAAAAGCTATTTTTAAAACAAGTGATGATGCAATTTTTGCACCAGATCATAAATATACACTAACAAAAATTGAAGCTGATAATAAAAAAGTTGCTAATATTGATGAAATTTCACCATTAGATCGAATAATTAATAAACAAAAAAATGGTAATGTTAATGCTGATAATAAGCATGAGTTTAAAATACCAGATCAAAAAAATAAAAATTTAATTGCTATTTATAAAGATAAAAATAACAATGAAATTCATGTTCCAATCAAAACTGATGATAAGGGAAAAGTTATTGTTGATCCAAACAATAATTTATTTGATCCTAACAAAATTTATGATTTTGATAAAATTGTAGATTTAAATGAATATCCAAACAAAACAATATTAGATAGAAATAGTATTAACAAAGATGTTAGTGCAATTAATGATGGTGTCGATCAAGCTCGTAAGCTTGTTTTAAAAGCACCAGCAGTTTCTAACATAACAGCAAATGCTATTAATTTCCAAGTAAACTTATTTGATTTAAATAAACTATCTTATAATCGTCAATTTGCACTAACAATTGCTAAAGTTAATGATTTAAATGATACACAAAAATACATTGCAACTTATGATCCAAAAACTAACAACTACAAACTAAATTTTGATTTTACACACTTAGATGCAAACACAAAATATAAAGTTATTGATGTTGAATTATTAAAATTAAACAACAAGGAAAAGCCAATAAAATTAGTTAAAGATGATGTTTTAAATTTTGAATTCACAACAAGCCCAGCAACTACTATTAATCCACCAATTTGAACTAAATTTGATGCGGTTGTTAAAACTAATAATGATACAACTATCACCTTTGAAATTGATGATAAAGATAATATTTTAAAAAATGATCAAAAAATCTATGCCCAATTAGCTTTAATGGATGATGATTTATTAGATACAGATGTTATTAACCCTTTAGTTAATTTTAGTAAAACTAATAAAATCGCTTCAATTAATGGTCTTGATTTAAAAGGAAATAGTAAATATAGTATTAAAAACTTATATTATTTAAATGACCAAAACCAAAAGGTGTATTTATTTAAAAATGATGTTACAAAATATGAACAATATTTTATTACAAATCCTCATAAGATCAATTTATCATTTAATAAAAGCGCTGCAGAACAAGATATTTTTGCAGACCATGCTAATTTATTTATTGATTATAAAGATCATGATCAAAAATTACGAATTAATGATGATGTTAAAATTTATTATCAAAATATTGATAACATTAAAAATGAACTACAAATTGGATATGGAAAAGTTGTTGTAAATAATAAAATTAAATTCAATTTGGTTGGTTTAAAAGAAAAAACAACATATGTTATTAAAAAACTTGAAGCTTTAAATAAGAGTGCAAGCAGTATTGTTAATAGTGAATTTGATTTACTAGATCCAAATACTAATTTTAGTACATCTAATAAAAACACAACCCTAGTGGGTCTAAATTCAATTGATAATTGAAATAATGATCAAGCTCCTATAATAAATGCCAAAATTAATATAGGTGATGATTTTCAAGATAATCAACAAGTTAAATTAATTTATGTATCAAATGATAACAAAGAGATTAAATCAAAAGCAGTTACTTTAATCAAAGGACAAAGAAATTATCAATTCGAATTTTCAAATTTAATTAAGAATCGTTTATATACTTTTTCTAAAATCGTTTATGAAACTAATAATCAAGAATTACATAAGTTAGACACGTTAACTCATCAATTTAGCATTAATCCAAGTAATAATGCAATTTCTTTAAAAAATAACACTAATATTGAAGTTACAAACCGTATATTAGTTAATAATGACCAATCATTAATTTCAGCAAAGATTGAAGTTGATGACATTGATAATGTTTTAACTACAGCTGATAAACCAAATATTGTTTATCAATTAGAAAATGATCTTGGCACAGAAAATAATTTAAAATTAGCAAGTGATGCACAAGTAGTTGTAGAAAATGATAAAAAATACTTAAAATTCAATTTAGATGGTTTAAAAGTTAATCAAAATTATGTGATTAAACAAATTAGTTTAACTCAAAAACCAACTAATGCTCATTTCAATTTTGCAAATAATAAAACTAATAACATTGTTTATTCATATGATGAACAAAATAAAATTAGTTTATCAAACAATATTATTGCAACATCTTATAGTCTACAAACGATTAATAATAATAAAAAATCAGTTGATATTAAGGTTGGTTTACAAGCTGATAATCAACTTTTATTAAATCAATATTTACGTTTAAAATTCAAACGTTTAAATGATAATGCAATTGTTTGAAGTGAACCAGTACAATTTGTTACAAATAATAATAATAATAATAATGAAATTACTTTTAAATTAACTAATTTAATCAACAATCGTACTTATAAACTAGAAGGTTTATATTATTTTGAAAATCAAAATTTAACAGATACAATAATAAATAATGAAATTAATTTTAAAAATAATGTTAATAAGCAAAATATTGAATTTGAACCATCAACAACAAGTATTGAATATGATATTAGTAATGCTAGCAAAGAAGTTACTGCTAATAGTGCTAAAATAACTTTTAAAGTTAAAACAAACGATCAAGCATTAGAAAATGATCAAATTGTTGAAGCAACTTTTGTTGCAACTAATAATTTAAATGATAAAAAAGTTGTTGAAACAAAATTAACTAATGTAAATAGTTTATTTAATGAAGGACAATTAGAATATAGTCTAAGTGGTTTAATTGAAGAAACTACATATCGTTTAATTAAAGTAGTGTTTAAAAATAAACCAAATAAGGCATACAAACCATTAAACAACAATAACATTATTTTTGAACATAAAGACAATACTCAAGCTAAAGAGTTCTCTACAACAAAACAAGATCACAAAGTGATTAGTGTTACATCAAGTGTTTCAACCAATACAACACAACAAGAAATAACTGTTAAAATAGATGGTATTCAACGTGCATGAAATAATAAAAAACTTGAATTAGTTTATGAATCAAATATTTCAAATGATCTTGAAGTAAAAACATCAGTTGATAACAATAATAGTGATGTTGTTTTAAGCTTTAATAAAAAAGAATATAAATTAACATTAAATAATTTAAAACCAGGACGTCGTTATAGTTTAAAGAAAATTAATATTAAACAAGTTAATAATAACCAAGATCTTGAATTTACAAAAGAATCAAATGTAAGTAACTCATTTGATGTTAATTTACAAACTCAAATTACTGCATCAAGCATTGAAGAAATTAACGATCGTGCTGCTAATAAATTACATGAAACAAGTATTAAAATTAATTTAAATGATCAAAACCAAATTCTAAAACAAGGTGATAGTGCTGAAATTACCTATGATAATGATCAAAAAATTAATGCCACTGTGATGGTTGATTCACAAAATCAAAAATACTTACAAGCAACATTAACAAATTTAGTTTTTAATCAAGATACTATTATTAAAAAAATTGAATTCAAAAATTTAAATCAAACTTTTACTAAAGTAGGTAAAAATAACACTAATGTAATCTATGATGAAAATGATGTAAATAAATTAAAAATTAATAATGATTTTCAAATTGTGGGACCACTATCAACTGATACTAATAACACACAAAATATAGTGGCTAATAACAAACATGTAATTTCTTCAACACTAGATTTTAAAGTTAATCCACATATTAGTAAGAATTTGAAATTTAAACTTAAATTTCAAAATATGAATGGTGAAGTTGTTTATAGTCCAATTTTAACTAATAGTTCAATTATAGTTAATAACAATAAAAACGTAATTAATTTTACTTTAGCTAATTTAAAATCAAATCAATTATATAGATTAGTTGATGTTTATTATATTGATGATAATAATGATACTATTAATGACAAAAACAAAGTACCAAAAGCAAATGATGTAACTCGCATAATTAATATTGCAGCTGATAAAACAACAATTACTAGATCAAACAATAATTGAAATAGTACAGCAACAAGTTCACAATTTGAATTTGAAGTAAGTAGCGAAGATGGTAATGATGTATTAGAAAATTTAAAAGCAACAATTATTTTTAAAAAAGATCAAACATTATTAACACCAATTGAAGTTAATATTACTAAAGAAAATGATAAATATTTGATTAAAGGTGAAATTACTAATTTAGAACCAGAAAATACGTATACACTTGAGTCAATTGTCTTAGCTAAACCAAGCAAAACAAATGGCCCATTAAATGTTGAAATCACAAATAAAGATAACATTTCATTTGAAACAAAAGCTGGTAATTATAAAGTTATTCAAATTAAATCACAAAATCCATCCACAGCAAATACTAAACAAAAAATTACTTTAAAAGTTGATGGTGTGCAAAATGCTTGAAATCAAAAACAATTAGAAATTACTTATACTTCAAGTGATAGTTCAACAAGAACTGCAACAATTACATTAGAAAAAAATAAATTTGAATATGAATTTGAATTAACTAACTTAGAAAAAAATCGAACATATACATTTACTAAAATTGAATTAATTAATGGTAATAGTACTAAAACAGCATTTAATAAAGATGATTCAGTTCAAGATAAATTTATTGTTTTAAGTGATAATCAAGTTGGTGTTAATAGCATTACTGAAATTAGTGATCGAAATTTTGATAATTTAACAACTGCAAAAGTTTGATTTGAATTAAATGATGCTGATAATATTTTAAGTAATGATGAACAAGCTACAATTACTTATGATAGTAATAGAACTGCTAATGCAAAAGTAATTACTAATCAAAATAAAAAATATCTTGAAGCAACACTTGATAATCTAGTTTTAAATCAAGATACTATTATTAATAAAATTGAATTTAAACAAAAACCTCAAAATGCAGGTAAAAATGTTGGTATTAACAATACTAATGTTATTTATGAAGCAACTAATTTATCAATTAATAATAATTTAAAAATTAGTGGTCCAAATAGTAATATAAAAGAAGTTGAAGCAAACAATAAAGCCAACGTTTCAATTAAATTAGAACTAGACACAAATGATCATATTACAAAAAACTTATATTTAATTGCTAAATTTGAAAGTAATGATGGTCAAACCGTTTTAGGTTCGCCAATTTCTTCAGATAGAATTGTTAAAAGTGGTAATAAAAAAGAATTAACTATTAATTTAAATAATTTAAAATCAAATCGACAATATAGTTTCAAAGGATTATATTATGTTAATTCTTCAAGTCAAACTAGTCTAAATGACAATAATAAATTTGTAAATAATCCAAATGTTGATCATAAAATAACTATTAAACCATCAATTACTACAATTCAAAAAGATGGTGATTGAACACTCCCACAACCTAATGCGCAAAAATTTAAATTTAATATTAATTCAGATGATAGTAGTGATTTTAGTACAGATTTAAATGTAACCATAACATTTAAAGATCAACAAAATACTAAAAAAATAATCACAACTAAACTAAAACAAAATAATCAAAATCAGCAATGATATGTTGAAGATACTATTAGTAATTTAGCTTATAGTGATACATATAAATTAGATGATATTTCAATTAATAAACCAAATAATGCTTTCGCTGATCTAAAAATTCAAATTCAAGATAAAGAAAAAATTAGTTTTACAACTCAAAGTGGACCTACTCAATTAATTAGTTTAAGTTCTAATAATTTAACTTGAAGTGATGAACTTAATTCAACAAATAGTCAAACTATTACTGCAACAGTTTCTGGCGTTAATGATTTATATAACAATCGTAAAATTAAATTAGTATACGAATATACAAGTGCTAATAATCAAAAAACACAAATTGAATCAAATGAAATAACTTTACAAAAAGATCAAACAACTTATGTAATAACATTACCAATCAATGTTGCTAATCGTCAATATTCATTTAAAGAAATAAAAATTGAAACTACTGTTAATAGTAATAATTATGAAGTAATTAATAATCAAAATAGTGTATCTAATAGCTTTAATGTAAATCCATCAAGAACAGAGTTAGTTGTTGAAAACAATGATTTAGCAACAAATATAACAAGTACAAGTGCAACAATTACATACAAATTAAAATCTAAAGATCATGTTTTTGCTGTTGGCGATAGAATTGTTGTTTATTTTAAATCTAATGAAGATGAACGTCAACAAATATCATATACAAAAACGATCACAAGTGTAAATAATGAAGGAACTGAAGCTACAATTACTTTTAGTACTGATGAAACATTAAGAGAAGAAGCCAATTACAAACTAGTTAAAGTTGCATTTGTGCGAAAACCTACACTAGCTCATACTAATATTAATAGTAATGAAAACAATGTTGTTTTTGAAGATGATAATTCAAATCATAATTTTAAAACATTAACTGTTGATCATAAGGTTACAAGCATTAATTCTAGTGATAATATAAGCACAACAACACAAACAGTAAATATTAATATTGATGGTATTCAACGAACTTGAGCTAATCAATTAGTTCAATTAGTCTATACTTCAAATAATAATGAAGAAGTTTTAAGTAACCAAAAAACATTAGTTTGAGGAACAAATAATTATAGTTTTGAATTAACTAATTTAAAACATAATCGAACATACACTTTAAAAGAAGTAAAAATTATTAAAAATAATTCTAATGGTCAAATTTTCCCATTAGCAACTAATGTAAATGATAATTTTACTGTTAATCGATCAGCGCCAATTAGTATACAAAATTTAGAAGAAGTAGCTGATCGTCAAGCTACAAATCTAACATCTGCAACAATTAAATTAACACTTAATGACCCTGATAATGTTTTAAATACTAATGACCAATTATCAATTAATTATGGTACAAACAATAAAACTATTAGTGGTAATGTAAGTATTGGTAATAATGGTGAAAAATATGTTCAACTAACATTTAGTGACTTAACACTGAATGAAACAACCATTATTAATAAAATTAGTTTTAATACTAAGCCTAATAAAGCAGTACAAAATGTTGGTATTGATGTTTATGATAATAATAATGTTATTTACGAAAATAAAAATAGTACTAATACATGAACCATTAATAATGATTTAAGTATTAGTGGACCTTTAAGTACTGAACATAATTATCAACAAGAAATAGAAGCAGCTAATAAAAATGTAGTTACTTCAACACTAAAACTTAAAATTAACAAGCGTATTACTAAAAATCTTAAATTTAAGCTTAAATTTATTGCAACTAATGGTGATATTGTTTATAGTGATTTAATCGATAGTTCTAAAATAAAAGAAGATAATGGAAATCAAATTATTAATTTTGCTTTAACTAATTTAAAGTCAAATCAATTATATACTTTTAATGGTTTGTATTATTTTATTGATGCAAATAATACAAATGTTGTTGAAAACCAAAACTTTGCAAAAGCTAATAATGTTAAACGAACAATTAATATTACACCTGGTGATACAACAATCACGAAAGCAGATGATTTTAGTAGTAATATAACAGCAAATAGTGCAAAAATTAAATTCGAAGTTAGAAGTGGAGATGAAGGAGCTTTAAGCAATACAATTGAAGCAACTGTTGTTTTTAAAAATCAAAACAATGAACAAACAACTCCTATTAAAACATCTTTAAAACAAATTAATGGTAAATGAATTATTGAAGCTGATATCAATAATTTAAAACCTGAAATTACTTATACATTAGATAGTATTAGTTTATCAAAACCAACAAATACTGCTAACAACCTTAAAGTTAATATTAATAATAAAGCAAATATTAATTTTAAAACTGCTGCTGGAAATTTTAAAATTACTAACATTACTTCAACACAAACTTATATTAATACTAATCAACAAACTATTAATGTTAATGTTGATGGAATTCGTGATGCATGAGTTGGTCAAAAATTACAATTAGTTTATACTTCAAACACTAATGAGGAAATTAAATCTGATGAACAAACTATTACAAAAAATAATTTTTCATATCAATTTAATTTTAGTAATTTAGCTCATAATAGAAAATATACATTTAGTAAATTAGTTATTAAACAAACAAATAGTGCAGATACTAACTTTGATAATACAAACAATATTACACATGATTTTATTGTTAAATCTAAAACACCAATTAGTGTTGTTGATATTAATGAAATAGAAAATCGTCAAACTAATGCTTTAACAAGTGCAAAAGTTAAGTTTGAATTAAATGATTTAGAAAATGTTTTAAGTAATGATGAACAAGCGCAAATTACTTATAATACTAACAAACAAATAATGGGCAAAATTATTGTTGATAGTAGTAAAAATAAAAAATATCTTGAAGCAACACTTAATGATTTAGTATTAAATGAAGACACTATTATTAATAAAATTGAATTTATTACAAGACCAAAGAAGGCTGCTGATTTTGTTGGTGCAAATAGTTCTAATACAATTTATGAATTAACAAATAATACCCAAAATAATCAAAAACTTAAAGTTAATAATGATTTTATTATTAGTGGTCCTCTTGCAAGTGATAATAATGCAATTGAGACAAAAGAATATGAACAAAAAAATAATGTTAGTTCATCAATTACACTAAAAGCAAATCCATACATTATTAAAAATTTACAATTCAAATTAAAATTCACAAATCAAAATGATGAAAGTGTAGAAAGCGATTGAATTAGTAATTTTAATAGTCAAGAAAATAGTCATACAATTAACTTTAATTTACAAAATCTAAAATCAAACCAAACTTACTCATTAGCAAATATTTATTATCTTGACAAAACAACTAATCAAATTATTAATTCACAAACCGTTTCAAAAGTAGATAATTTTAAAAAGCAAATTGTTGTTAAACCAGGTAAAACAATTATTGCTAAAGAAGGTAGTTGAACTGAACCTAATACAAATAATGAAACTAATTTTAGTTTTAATATTAATTCTCAAGATGGTAATGATGTTTTAGAAAATGTTAAAGCAACTATTAAATTCAAAAATAATACTACTCAACAAATTATTTCAACAATAGCAACAATCACTAAAGAAAAAGATAAGTGATTAATTAAAGGAAAAATATCTAATTTAGACCCAAATACAAAATATAGTTTAGAAACTATTGAATTAAGCAAACCAACTAAAACAACTAATAATCTTATTGTTGAAATTACAGACAAAAACAATATTAGTTTTACAACTCAACAAATAGATACACAATTAACTAATTTAGAAGTTAAAACAACATCACAAAATGACTTAAATAAAAAAACAATTACTGCAACATTTTCAGGTGTTAATGATAACTTTAAAGATAAACAAGTTAAATTAATTTATAAAGATAACAATAATCAAGTTTATGAGAGTGAAACAATAACATTAGTAAAAAATCAAACAAAATATGATTTTAATTTACCTAGTTTAATTCCTAATCGTAAATATACTTTTGATTCTATAAAAATTAGTCAAACACCAAATAATAATCAGTCATTTATTGATTTAGCAAAAAATTCTATAACAAATGAGTTTACGATTGCACAAAGTACTACAAATATTGAATGAATTCAACCAGAAAATGATGAGATAAACGCACGTGGAGCAAAATTAACATTTAAAATTAAAACACAAGATGCTATATTAACAACTAATACAAAAGTTGAAGCTTATTTTGCTCCAGTAAATGCAATAAATGATCAAAGTCAATGGAAACTATTTACTAGAGATTTAACAGATATTGATCAATCAAAAAACATTGGTACTTGAACACATGATTTAAGAAATCAGACATATTTTGATGAAGAAACAGAGTATGTATTAGTTAAAATTAAATTCAAATATAAGCCATCTTTAGCTTTTGCTAATATTAATAATAATGCTGATAATATTATTTATCAATATACTAACGCTTCACCTAAATATGCATTTACAACAAAAGTTGGGGATCATAAAATAAATAGTATAACTTCAACTTCAACAACAAATAATTCTGCTCAAAATATCAATGTTACATTAACTGGAGTTAAAAATATTTGATTAAATAAACAAATGAAATTAACATATCGTGGAAGCGATAATGAACTTATTTCATCAACAACTAATATAGTAAAATCAAAAACTTCATATACATTTACTTTAAATAATTTAAAACATAATCGTGTTTATCATTTAGAAAAAGTAGAATTAGTTGATAATAATACAACAACTCCTTTCGCTAAATTAGATAATATAACTAATTCATTTATTGTAGAACGAACTGAAGCTGTAAATATTAGTGGTATTGAAGAGTTAGAAAATCGTAATCCAAATAATTTAAATAGCACAGTAGTTAAAATTAGTTTAATTGACACAGATAATGTATTAGATGCTAATGAAGTTGTAAATATTAGTTATAACAATAATAAAACAGTTCATGGAACTGTTAAAATGCAAAACAAAAATAAATATATTGAAGCAGTATTTAATGATTTAGTTTTAAATGAAGACACAGTTATTAAAAGTATTAATTTTGCTAATAAACCATCAAAAGCTGGCGAAAACATTGGTATTAATAAAACTGATAACAAGATTTATGATAATGTAATAAACACAAACAAATCATTAATAATTAACAATAATTTTAAATTGGTTGGTCCAACTGTAAATAGACAGATTACTAAAGAAGCAAATCAAAAAACAAATATTACGTTTGATTTAGATGTGAATATAAATCCAAAAATTGCCAATAATTTAAGATTTAAAGCTAAATTTAAAGCTACAAATGGTGAATTTGTTGAGACAAATATTGCTACTATTACATCAATTAGTAATAAACAAAAAATTAATTTTACATTGACTAATTTAAAAGCTAATCAAATGTATACTTTAAGTGAGCTTTATTACCTAACAAATCCTAATGATAATACGATTGTTAATAATAATAAAATTCAAAAAGAACCAAATGTTTCAAGAATGATTTTGGTTAATAAGGGATCAACATTAATTAGCAAGTTTGGTGAATGAAATGAAATTAGCGATACAACAGCACAATTTAGTTTACTAGTTGATTCAGAAGATAATAATAATGAATTAAAAAATGATGCAATAGCTACAGTTAAGTTTAAAGACAATCAAAATAACATTAAAACAACTACTGTTAATTTAACTAAAGAACAAAATCAATGATTAATTAAAGGGTTACTAACTCAATTAAATCCAGAAACTGAATATCATTTAGAAACAATTAGCATTACTAAACCTAGCAATCTTGTTCAAGATTTAGATATTAAAATTCAAAATAAATCAAATATTAAATTTACAACTGAACCAGGAGCAACAGTATTGACTGCTTTAAATGTAGATAATAATGCAATTAGTACTGATACACAAACAATTCATGCAACTTTTTCTGGAGTTAATAACAAATATCAAAATCGTAAAGTTAAGTTGATTTATAAAGATAATAATGATCAAATTTATGAAACAAATTCAATCACTTTATCTAAGAAAAACAAAAATTATGATTTTACTTTAAATAATTTAATCGCGAACCGAAATTATATTTTTTACAAAATACAAATCACTGATATAGCTAATGATACAACTTATGTTGATTTGAAAAAAATCGATAGTTTAACTAATAGTTTTAAACGTAATCCATCATTAACAAGTATTGAATTAAATCAACCAACTAATGTTACACATAATAGTGCTATTTTAAATATCACACTAAAATCAGTTGATAAAGTATTTGAAGTTAACCAACAAGTTATAGTTTATATTACAAGTTCAGATGGACAAATACTTCATTGAACTAGATGAATTAATCAAGTTAATAATGATAAAACTCAAGCTACAATTAATCTTGACACAGGAATGTCGTTAAACGAACAAGTTGAGTATAAATTACTAAAAGTTGAATTTGCTCAAAAACCTAATTTAGCATATGAGAAATTAAATAACAATAACATAATTCAAAATAGTAATAATAATGCGAAGTTCACTACAAATATTGGACCAACAAAACTTATTAGTCTTTCATCAGTAAGTACAAATAACACATCTTGAAAAATTAATGCTGTCGTTTCTGGTGTTAATAATGATTATGTTGGTCGCAAAATTAGATTATCTTACAAAGATAATAATGACAATATTGTTAGAACAAATGAATTGTTATTAGAAGAAAGAAATAAAAAGAATTATCAATTTAATTTTAATAACTCATTAATTACTAATCGTGAATATAGATTCTATAAAGCAGAAATTACAAATGCAAAAAATTCAAATACTTTTATTGATTTAGAAACTAATAACGGTGTTAACAATAGTTTTACAATTAACCCAGGTAATACACATTTAATAATTGATAATAGTAAATTTGCTACAAATATCTCAGAAAATAGTGCAACAGTTTCAGTTAATATTACTTCGACTGATGGTGCTTTTGAGAATAATCAAGAAGTTGAAGCCAGATTTGAATCAATTGAAAATCCATCAGAAACTAAAGTATTTAATGGTGTATTAACTAATGTTTCAAATGACAAAAATAATGCTACAATCACTTGAAACTTACAAGGATTAAAACCCCAAACGGAATATAAATTTATATTTGCTAAATTTAAAACAAAACCTCATTTAGCTAAATGAGAATTAACACCTAATAAAAATAATGTCGTATTTGGTGGAACTCAAGAATTTAAATTTAGAACAATGTTTAAAAAACCTAGATTAATTAAATTAGAAAGCCATAATATTGATTTTAATCTTGAAAATCTTTTAATGCCAAACAAAGAATTAGGAAAAATTAAAGCTACTATTTCTGATTTCAATGATAGTTATTTAAATAAAAGAATTAGGTTAATTTATGAATCTAATGATCACAAAGAATTATCAACTAATGAATTGGTTTTATCTAAGAATAAAGTTGAATATGAATTTGATGTTAAAAAGATTACATCAAATAGAAAATATACATTTAGAAGAATTGAAATTTACAATAATAAATTTGAAGTATTGGATAATGTCAATAATATTACTAATGAATTTATCTATTATAATGCACCTACAACTATTACAGTTCCAGGTGATTTAGCTTCAAATATTAGTAATAATAAGGCAACATTTAAATTAGGTTTGAATTCAATAGATAAAATTCTTGAAGATGGACAAGCAATTAGTGTTGTTTTAGCTAATAAAAATTATCCAAACGATACAAGCAAATACAAAACATATACAACATTTGTGAGAGAAACTAGAGATGATGGAATGCACGCAAATATTGTTCATGATTTAAATAATTTAGATGAACATAGTACATATTTTATTAAAGAAGTTAAATTTATAAATAAACCAAATAAAATTATTGAATATCCAAATTGAAATAATAAAATTTTTGATTCAACTAAAAGTTCACAAACTTATGAATTTACTACTAAATGAGCAAATTATAACGCAGAGAAAAAAGAGCCATGAAATGATACTACAAGAAATAGTGCAGAATTCAAATTTAAATTAACAACAAGTGGTATTATGTTAAATACAAATACACATTTTAAAATAACTTTTAGAAAAGACAATGATCAAAATCATCAAGAGAAAATTTATACTGGAAATTTATCTCTAGAAGGTAATGAATGAATCTTAAAAGGAAAATATGAAAATTTAGATCCTAATAGTACATATACATTAGATTTTATTAAATTATCTGTTAATAATAACTTTGACAATGATAGTGTAAATGTTCAAATTGCAAATAAAGATCAAATTAAAATTAAAACAAAAATAAATGAACCAATAATTCAAAGTATCACATATGATAATAACAATAAAGCAAATATTAGAAAAATTAAATTAGAAATTAATAATATTGATCCAAGTGAAAACGGTGATCAAATATTATTAGAATATACTTATGATAATGGTACTCGAACTAGAATGACAAGTTCATTAACTTTGGATAATAACAAAAAAGACTATGAAGTTACAATTAGTAATTTAACTTATAATCGTGAATATACATTAAAAAATATTATTAAGATTAAAAATGGCCAAACACCTAAAGTATTAAATATTAGTAATAGTATAAATAAAAAATTTGTTGTAGACCCAAGTGTTACAAAAGTTTCAAACTTTAAAAATGACAAGTATGAAATAAATTATGCTAATTTAACTTTTACAAGAACTTTAGAATTTGATGTCAATAATTCTGATGAATGATTTATTCCTACAAAAATTCAATCATACAAACCACGTTCTTTAATTTGAAATATAAGTAATAATAGTCGATTTAATGATGATAGAATTGAAACAAAAATTATCAATAGTGATAATAAACCAAAAATTCAAATTAAAATGAAAAATTTAAGTTTAAATGAATTTAATCAAAAATATCAAATTAGATCAATTTATTTCTCAGGGGAAGTTAATCATAATTTAAATCAATTATTAAATAATGAAGTTCAAAATTCTGTTTATTTATGAAATTCTCGTTTCCCATATTTAAAAGAACAACAATACTTTATTGAAGGAACTGGTGATATTAAGGGAGTTCTAGAATTAAATAGTACTGATAATGGTTTTGGTGCAGATAATAATAGACAATATAATTATGTGAAGTTTAATGGTTTATTAAAAGATTTACAAAAATTCTCACCGCAAGGAAACAATAACCAAACAATTTTTGCTAAGTTACTTTTTGTTAATCTAAATAACAATAAACTTTATCAGACAGGTTTAGGAATTATTAAAGATGATAATAAAGTCAATAATTATGACAATTCTTCAAACTTCATTGTTCCAGCTAATGAATGACCGCCAGGAAGATATCAATTATATGGATTGAAATATTGATCAAATACTGGTAACTATAATGAAAATAATGCAAAAATCGCTTTAATTCCTTTAGAAAAACGTTCAAGTAAAATTTGAGATAGATCTTAATATCAATTACTATAAATCTATTCTTATATTAATATAGGGATAGATTTTTTATTTTCAAAAATATGGATTTTGCTTTTATTTAATTTTAATAATAAAAAATTTACCCCAATATTTATTGGGGTAAATTAATATTTATTTACAAATATAGTTTTTTACTTATAAAATAAGATGATTATAATTTTGGAATTTTAATACCATGTTTTGCGTAAAAATCTAGCATGCCATCATAACTAATTTCAGAATATCCTCCATCTTTAACATTTGCCCCTGCGAAGGATAAAATTAAACCAACTAAACGACGTTTGTCATCATCATTTTTAGCTTTATCTCATATTTCTTTAAAAATAGTTGAGGCATTTTGTGCAGTATATGTTGATTTAGCAACAGGATAGTAATAATATGAACCATTTGCTGCTTTATAAATGGTTCCCCCATTTACTTCTGGACTAATTGTTTTTCCATTTATTAATTCACCTTCATTACCCCTAGCGTTAGCAAGATTTTTAGGGTTTATATATCCATCAATTTTAGGATCTTGGTTTAAACTTTTAAATAAAGGATCTTGGATCATTTTTTGAACTAATTGGTCATATGTACTTACTTTATTAGTTTCATCTGTTTGTGCTAGATTAAAAGCATCAATTAAATAGTGATAAGCAGATTCTGAGATTCCTACTACACCATCAACTAGTGAACCTACAGTGTTTACACCTAATTTATATTTATCGTGAACATCAATTTCATCACCAATTTTGTTAACATGTCCTTGAAATTGAGTACCATTTACAGCGTTTTCTAGTAATTTTTCAATTGCTACTCCTAATCGTTTAACAATTGAAAAACGAATAACACCGTTTTTATTTCCGCTTATATTAGTATTTTTAAAAGTATTTGCTTCTTTATTAATTGTCTTATCGTTTTCTTGTTCAGTATCTACGCCTATTATTGCTACTTTGTGTGAAGTTGTACTAATTGCTTCATTAACTGCTAAACTCATTTGAGTAATTGCTACTGGTAAAATTAAATCAGCTTTTTTAGAAATTAGTTCCTTAAGGATTTTTTTAGCATTTTCATTATTTTCATCAAATCCTCCAGCACTACTTTGTGAAGCTTGAACCTCATTTACTTTGATTCATGTTTTAGACTCACTACTTCCTTCTTGTAATACTTTTTTGTTTGCTAAATGCTTGTTTGCTCATTCAATACCCAAGGCAAATCCTGCTAAATAATTAGTAGTGTTTTTAGCATTTAAAGCAACATATCCTCCCCAGTTAAGTTGGCCATCTGTTGCAAAAATGTTTTGGTTTGCATTTAGCATATAAGCGGCAGCAATTCCACCTAAAAAAGCAGCATCATCAACTTTAAAATAAACACTTGCAACACGATTTGCACCATCAAATAAAGAAGGATCATTATTTGCATTATAAACTGTATCTAATAAAAAACCAACAGCATTTTTATCAAATTTAGCGTTTTTTATTGCGTCTTGTAGATAATTGCTTTGTGAAAACCCTGGTAATAAAAATGCACGTTTTCCATCTTCGACCATACTTTTATGGATTGTTTGAAAACCTTCGCTTGTATCAGATTTAGGCGCTTTTCAATAATAATGTGAATCATTTAATTCACTATTAGAAGAACTTATTTCATTTTTAACATTAACATTTGGTTCATTATTTTTAGTAACAGAATTATTGTGTTTTGCACAAGCTGTTGCTACAACTGTTGTTGTGATGAACGCTAGTGTACTTAAAGCTCCAATTACTATTTTTTTAATATGTTTTTTTATCATATTTCTACTCTCCCCTTATTTATTTTTAAATTCCAACAATTTTACTTTTTTTAAATTGTCCAAAAATACTGTATAAATGATATCATATTTTAAATTTTTAACTTCCCAACACAATTTAAAATATTAGATTAGCACTATTATTTAAGTTGAATTATTGTTTACTATGCTTTTGCTTAATAATTTTTTTATAAAAGGGTTTTAGTGTAGTAATTTATAAAATATTAGTATTTACACAAAATAAATTCAAAAAAAATGTGTTTTAATTAAATTATGTTAAATCGTTTTAGAAAATAAATATTAATATGAAAATGAAAAATAATAAGAAAATACATTGAAAAAAGCATATTAAAGTTGCTTCTTTTTTAATATGTTTAAATCTATGTTGATCAGCTGTCATAGTTGGAATTGTTTTAAGATTTTATGATAAGGAGAATTCAATTATTTTTGTAGATCAAAATAATGATTCTTATTGATTAAAAATTCATGATAAAAAAGATCTTAAAACCAAGATTATTAACCATTTACCACAAGGTAATGTTTATCGATTACAAAAAAATAAATTGTCTAATTCCATAATTGGTTTAACTTTATCTAAAATAGATAAAACCAATGAATATTCAAATACTGGTGATATTGTTATTAAAGTTAATTTAAAAAACAAAGCTAAAAGGAATCCGCAAATTATGGGTGTTTTTGTTGATGAAAAGCATGAATATCATTACATACAACCAACTATTAAAGATAAAGTTGCTTATTTTAATACATCAAAATTAGTAAATAATCATGATTATCGTTTAGTAAAAATTTTTGACAAAACTGATTATAATCACGTTTTAATTCAACAAAATGAGTTAGCGATTGAACATCAAATTATGATTAGTAAACCACCTAAAACAGCTTCTTTTATTAACCAAAATAATCAAAAAATATACCGAATAAATGTAGGAAAGTGATTATTTAATTCTTCTCTTAGTTTGCAACTAAAAGATTTAAATAATCAAATTTATAGTATTGATGGCTAAAGTAGATGTAGAAGGAAATATTGATTTTGATGTAAGCAAATTAGCAAATAATAACTTCTATGAATTAGTTAACATTTATAAAAATAACACGCATCCTTTAGTTAAAGTTATTAATCCAATTAATATCGCATATCATAATAAAACTATTAACATTTAAATTGTTAATAGTTTAAATCAACCATATCAGTATACAAAAAGTGGTGATATTAATTTAATTGCTAAAGTAGCGCCATATTATGTTAACCAACAAGTATATGGGATATTTAAAGATCAAAATAATAAATTACATAAATTATTAGCTAAAGTTGATAATGATGGTCAGATTTGTTTTGATATTGGAAAATTAGTAAAACCAAACCAGTATCGATTAGAAAAAAATTGTTTCTGTTAGTGATGAAAATAGGAAGCGTAGAAAAATATGGGGCTAATCCAAATAAGGAAAAAAATAAGTTTAAAAATTTTCGAAAGGAATTTTATGGGTGCTAATAAATAATTTATGTTAATGCAGTAAAAGGCTAAATTAAACAATGATTATAGTCTTGTGGATGCATTTGAGAGAAATTTAAATTGGAGGAAAAATTATAGTAAAAAAATCTATGACAAATTAAGCAACATAGCTATAAACAAAATTTTAATATCAAAAATAGTTATTAATGAAAGTTATTGTTAAAAGCTAAAAATAAATAAGGTGTTGATTGAAGTTTTTATGGTTATTAAATCCTTTAAAAATAAAGAAATTAAAATCGTTGATTTAATAAATTTAGGATTTAAAAAAACTTCTATAAAAATGGCTATAAAAAGACTTATTGAAATAAAGGTTTTAGATGGTAAGTTTTATAAAAAATATAAGCTTGAAAAATTAAAAAAAATAGTACTAAAAAACTCTAAAGAAAATTACTGAATTTTAAATGGCTATGATGCCTTAAAAATCTTTCTTCTTAATGGTTTGAGTAATGCTGTTTATATGTAATAAATAGTCATAAATCAAAGCAAATAAATGATAAAAAATTGCATTGTAAAATAAACTCAAGAAAAGTGATTTTACGAAATGCATATTATGCAAAATTTAACTGATCAAACAGCAAAATATATTTAATGAACAAGGCTATAGCCAATTATTTTGGAGTTAAATATTTGTAAATGTTTGGTATATTGCATAGTCAAGAAAAAAATTTATAACAATAAAAACCGAATTTGGAAATTTAAAAATCAAATTTAATGGTTATAAATTTTTTTCTCAACGTTATTTGTTAATGCAAATTTAAAAGTTTTAAAGGTTTTTATAAGCAGTAATAAATGGCTCCCAGCATTGGGCTAATGTCTCTGCATTGTAGGTTCAAATCCTACGCGAGCCACCAAATAGTCTTTGACGTTTTAAATTACTACTTTTAAAAACATTTAAGTTTTGATAGTTACTGGCACTAGGTGTCTATTTTAGTCATAAATAAATTAGTTAAATTACTAATACTAATTTTTAAGAGTGAATATATGGTCAAAATTTTAAACCTAAATATATTAGCAGTAATAAATATAAATAATTATTAGTATTGCATTAGAAGGGAGAATAATATGAATAAAGTATGAACTGAATTTAATTTTATTACCCCTAATACAATATATAAAACCAAATTCGGTAATACTCACGATTTTAAAAGTTGAGTTAATTATGGACTTGTGGTAGATTACATCACAAGACAAGATAAATGCGTTGTATTTAAAGATGAAGAAAATAATTATGATGCTATGAAATTACTTCTTACTAGGGAAGACAAAATAAATTGATATAGACAACATTTGAAAATAAATCAAAATTCTAAACAATCGGGTCTTTATAATTTATTAGATAATGATCCAAAGGATGCAAATTTAGAAAATGTTAAAAACGATTTAAAAAATTTAAAAGGTAATTATTGAGAATTAATCGTTAATCCTGGGGATTTAGGCTTAGATAATAATATTTTGCGCAAGGATAAATGACATGAAGTTTTAAAACCTAATTTCGAAAGATTTTTAAAGGCTAATGCCTTTGATTTAGAAAATGTTAATGTTTTTTATTCTATACATGGAAATACTGATTATCATCATGTTCATATGTTTTGATATGAAAAGGAGCATAAACACAAAAAAGCCAAACTTAATTTAAATTCAATTAATGTTTTTGGATATAAAGTGGGCTTATCTATCAAATACGATGAGGATTATCAGAAAATAAATGCACTTACAAAAGATATTTGAAATACAAGAAAACAAATTGTAGGAATAATTGCCAACAATTTTGCTAATAGTAATGAAATTATTTCTGAAAGTAATTTGTACCAAGAATTCATTAAATCTTCAAAAGGTATTTTAGATGAAGTTTTAAACAAAAAAAATATTTCATATTCTCGTATTTCTGATGAAAATAAAGAAAATATTGAAATAATAAAAAAATTCTTATTAAATTTTGACAATGATTATTCAAAGCTCTTCAATTCTTATCAAAACCAAATTAAGGATTAAATGATTTAAATATCGAAGATAAATTTCTAAAATAAAGAATTTCAAAATCATAAATAATTAAATGGATGATTTTAAAAAAACAAGTTGGTAATAAGATAATCAAAACCTTACTAAATGCCTACAAACAAAATAATAATACATTTACTAGCCAAAAGAATTTCGCTTCATTTTTCAATGATTTCTAAAAATATTTAGTCTAAATAGATAGGAATAAGCCCTTAGGAATTTAGCAGAAAGAGAATTTTTGACTAAAGATTTAAAAGAACTTGAAATAGTAGAAGTTTTATCGCAGCAAACTTGTCCATTAGGAGTTTTATGGAAGAACAAGAAAAATTATCAAAATCAAAAATTATAGGAAAGACATTTCTTTCAATAATTGTTAGTACATTTTTGTTTTTTTGTTTTAACTTTTATTATTAATGTTTTTATGGTCCAGAATAAAAATCCTAGACATTTTAAACTTTTTTGAAACTCTACTATTACTTTATTAAAAACCAAACATAATATTTTTGTTTATATAGGTCTAAGAATTTCTATATTAGTATTCTGTTTCATTTTTTGAAGGCAAATAAAATCCCTTAGCAAAAAGATATCAAAATACTTATTTGGTGCAAAGGCTAGCCAAAATTGATTGTGAAATTATCAATTAGGTGAAGGCTTTAAAGCAAAATATAACAAATTATTTAGAAAAATGATTAATGCAGAAACAGAATCAAATTGATTATTTACTTTTGATAAAACTCAAAGATATTGATATGCGAACGATAAAGATGATAACTCTATTGTAAATGGACCTCATATTGAATTTATGGCAGAACTTTATTATTCAAAAAAGCAAAACTTAATAATTAGTGATCCCAAGAAGGAAATTATCAAATTTACAGGAGGCTTGTTAACTGAAAACGATTACAAAATTTATGTAATTGATTTCTCTGAGCCTAAATTGTCTCTAAAATGAAATCCTCTTTCATATGCCTGAGATTTAGCGGTTGATGGCGAAAGATTCAAGAATCTGACTTTCGAAGAAAGAATTAATAAATCATTAAAACTTATAAATAATGTTATTGAAAGCTTAAATTGAGGCAAGGGCGTTAATGGCAATATGTGGATTAGCCAAGCTAAAAAACATTAATTGTTATCACTAATTTCTTATTGTTGTTAGGTATTTATGATAAAAATATTATTAATAAAAATAATTTTAAGTTATAAAACCAGATAAAAGATTTTATAAATTTTGTCTTCTTCTATCTAAAATACAATGATGAATTTAGATTAGTAATGATATTAAATAATGTAAGTAGATTATTTAATTATTTATTCAAGGATTGTTTTTAATATAAATAAATTGGTCTTGTAATAAAATGCTTAAAAATTAGGAAATTTTTTAAAAATGTTAAAAAATAAAAAGAACGTAGTTATACTACAAATAAATTTAATTTTGTAACGTTCTCAAAGATTATCAGTGGGTGGGCTTGGATTTTTCCATTATTTAAAACTTTGCCATCAATTGGCAACTTAAAAATATTTTCTAATAAAAGATTATTATTTTCTTAATTCTACTGATTATTTAAAATGTGTGTGTGTGTTTTGTCTAGGCTATTTAATAAAGTCTTTCAACAAATCAATTAATCCTTCAGTATTAGTTCAAAAACATCTTTTATTCTTTTTTAGACTCAAAATAATTTCATATCTTTGTTGAAGAATCTTCGATTTTAAATTTTGTTGTTTTATAACCTCAGGATTAACTATTTCTTGGGTTGTTAGATCTAGTAAATAATTAGAATATACTCCATCAAGATATGAAATTAAAAAAATATTTTTAGGTAAATCGATATTTAATAATTCAATTAATTCCTTAATTTGTTTATCCTGAGCACCTCCTGATTCTTTTATGTGTTTTGCTTCTAAAAATATGTAATATTCATTAACTTTAAAAATCACGTCTAAATCTTTGCTTTGGGAACCAAAATCAATTCCTGGAATGATCTTTTTTTTTAGATCTTTAGAATAATATGCAACTGAATAAGGATTATTGAAAAATTCATCGCTGGTTTTTGAAAAAACGAAACCTATTTCAATTGCTTTCTCGATAACTTTTTTCTGACCGATAATTCCTAGCGATCTTTGTGCTCCTTTGTCAATAATAGCTTGAATTCATTCTTTAGGAGTATATTCACTTATATCTCTATATTGTAAATATAAATTAACAATTGATTTTAATGTCTCTGGATTTGATTTTATGGTGGAAGTGGCGGAGTCACAAGCATTAATAAAACAATTAAATTCACTTTTATTTCCTAATTCATTTAAAACCAATTCTTTTAATTTAGTATATATTTTTTCTGAACTCATGTTATTTTTATTTAAAGTCTTAATTGTTATTAACAATTCTTTTATTTGCTTGGTTGTTTCAACCATTTCGTTTAACGATTCGTGCATTTTAAGGGACATATATCTAGAATCTAAAGATTCTTTATTTTCGACTGATTTGATTAAATATTCTAAATTCGACTTTAATTTCATATTTTTTATATCCTTTGTTAGTTTCTAATTATTTATAAAAATAATCAAAAGCCTGTTCCATTATTGCTCTTGCTAATTTAAAATCCATTCTTCTTCTTCTTATGCCTGATTTTGTATTACCCATAAACGGAGTTAAAATTGTGTCCGCGGATTTGTTTCTAAGTTCTTGCAAAATACTATTGCATTTATTAATAAAAGATTCTCTATTTCTTTGTTTGATGTCGATATTTATTAATGTATAATGGCGAGAAGAACGCTTTATTTTTGTTTGTCAATTATATGGTATTTTTTCTTTAAAATCAAAATGAATTGATTTTTTATCATCTGTGCCGTCAATAGCTCTTATTCCTAGCCAACCGTGCAAATCATTGAATAAAATTTTCATAGTTTTTTTGGGAGATATTTTTAAGGATTGAAGAAACGACAATGTATTTATATAATTATTGTTTTTATAAATTTTAATTTTGTCATAAGTCTTTGGTATTCCATCAAAACAAGCAACACAAATTGGTGTGTGAGTATCATCAAAAGGATTTTCTTCAAGAATGGTAATCGATGATAAGAGATCTTTATTTTTAAACGAAGAATTTACAAAAGATTCAGGAATAATTGCGACTATATATTTTTGCGCTTCTATCATTCTCTCTAATGCTATTAAATACAAATCATCATATATACTATTATTAAAATATTTTGATAAATCAATTTTTTTTCTTGTTGCTGAATGCTTAGCTAAATATGGCGGATTAGTTATTATAATCGCATTATTTATACTAGGAATTTTTAGCAATGAATCATTTCATTCTCAACCTAGATGATTATCAATATCTAGTCCAATGATTTTATTAATTCCATATAATTTTGATATATTAATCAAATTGCCAGCGCCTGCAAAGGGATCATAAGCAATTGAACACTTAGAATTTAATATAAAATCAATAATTTGTGGTTTTAACCAAAATTTTTGTCTTGTAAAAAATTGGCCTAGTTTTATTTTTTGTTCATTTGCTTTGATATCTTTCTTCATATATTTCCTTAATTATTTATATATTTTTTAAGTCTTATAATGACATTTTTTACTATTTAATATTTGCTTATTTTTTATTTATTTTAAGTACATTATAATTATAAAGCTAAACAAAGGATTTTGCAAATTTTCGTCTTTTTCTACCTCATATAAATGGATAAATTAATGTTAATGCTAATATTAAACAAATTATAATGTAGTGCAATATTGAATTGTTTGTTTGTCCAAGATTCTTTTTCAACAGAAATAGTCATACCCGAAAAATAAAATGCTAAAAATAATCAAACGATAATTAAAAATATAAGATATCAAATAAAACTTTTTCAAGTTAGCCAAAAAGGTAATAGTAATTTTTGTTGTTTAGTAATTTTTTACCTCCACATTCATTTGATGGTTTAAATTTTAAGCATTTTTAATAATTTTTATAACTTGTATTCGCTCTATCTGTACCTCCTTAAGTTGCTTTTTTGAAATGCCGTCTTGTGCCATAATTAATGCTTTATTGTATTGCTGAGCAGAGCCAAAAGAATATGTTTTAGCGTTTTTTTATAGATTGGCTCCCTCTTAGCCTATAAAAGTATTTTGACATGTTGTTCCTTATAATTTTCTTGATTTGGATTGGGATTCCGGGGGTATGGGATGATTTGTTATATTCATTGATTACTTTGGAATCTTTAAGATCGGTTGAGGTCTTATCAAAATCAGATAGGGATGATTTAGAAGTTTCGTTTTCCTCTATTTATTAATTTTGAAAAAATCTTTGAGCATCTTCTGCACTTATCACCGAAGTTCTATTGTAAGCATTAATTGTAATATATTCAACATCATAAATCCTATTAATACTTATTATGTCTTCAATATCAAAAGCTCAACCTTCGGCTTCTATTCCCTTAGTTTTAAATTCTTGATCAGATAAGTCATAAGCTAAATAATAATCTCAAACATCTTACGGTTCGTGTTCTTTCCAATAATTTAATTCTTTGTGTAATTCTTCAGATGTCATAATTATTCTCCTTTTTGATTCCTTATTTGTTCTTGTAATTGCTCTTCATATTTATCTCTACATTCTATTGAACAAAAAGTAGTTCAAAAATTCTTAAAATACTTTAATCCTAGTTCTTTTTCACAAAAAGTACAATATTTATCTTTTGTCATATTTACTCCTTTTTATTTAGTTTAAGAATGTGTTTAATTATTTCTTGTAATACATTAACAACAATAGAATTTCCGGCAAACTTATAAATTTGACTATCACAGAAATTCTTAATTCTTTCATAATCTTGTTGGTCGTAGCCCATTAATAGCATTGCTTCTAATGGTGTAATAACTCTTAAATAATATGGTTTAATTATCTATAATCATTTTTGCTAAATTGCTATTATTAGGAACTTTGCTAGTTTCCACCTTAACAACCCCCTGATTATTTCAACGCATTTGTTTAGTCGTTATAGTAAAGGCTTTATTCTCTATTATTTTTATTTTATGGTTTGCTATGACATTAATCATTGAAGGGCAATTTGTTTTGATAAAGTATTTTGGATTTGTTACATTTGGTTGTAAAAAATCTTTTATATTTTTATATTTATTGTTTAGACAATTAAATGTATCTAAATTATTGAATTGTTTTAAATTGCTTTTATTAATAGAAACAGTAAAAACTCTATTTCTACTTTGGGGAATTTCACAATCTAATGAATTAATTATTTTGTAATGGCTAACATATCCAAGATAATTTAATGCCTTAATATATGCATCAAATATAGATTTATGTTTTTTGCTTAATACGTTTTTGACATTTTCTCAAATAATAATTTTAGGTTTTATTTGAGCTTCTTTAATAATTCTAATTGTTTCCCACAATAAACTACTTTGACTTTTACCTTCTTTGCTTCCTCCGATATTTTTGCCTGCACACGAAAAATCTTGACAAGGAGACCCGTGAAACAATAAATCAACATTAAGATTATTGGGAAGTGAATAATATCTTATATCTAAAGGTTTGTAAAAATCATTATATATTGCATTATAAGCATTTACTGTTTTTTATCAATTTCGACATAATCAATAATTTCAAAATTAGGAGGTTTGATGGTTCTAATTAGAGCTTTTCTAATAGCACCTATTCCGCCAAATAACTCAACAACTTTTATTTTATTTATATTTCATAGTAAAATTCCTTAATTAGATTTTTCAATTAACTTAGAGATATCTTCAGGAGGATCCAGCAAGTAAAGCAATATATCAACATTTAAATCGTGATAATTTGGTATTTTGTGCTCTTTTGCCTTGGATCAAAGAATATTAAGCAAATCGCTTTCGAGATCCTTGGCAACATATTGGTCTCACATTAAACTTAATGTTTCTTCTATTTTTTCTTTAATTTGGTTTTTCTTATGAGGTATTAATGTACGTTTATAATCATTTTTGAGATTTAAATAATTGTTTCAAAGATCTAGTTTTACAAAGACTATCTTCATTCTTTGAATTTTTTGTTCTTGGGATAAATCCTCAGGATTTTCAACATCTTCTAAATAAGTTAAATTGTTATCATTTAAAACTTTTGGGTTTTTATTATTTTGGTTTTTGTGTTTAGAATTATCAAATTTAATAAGTTTTTCTAAACTTCTAATATCAAACAATTCAAAATCCGCATTAGTTAGTAGCTTACTTTCATTTTTTTGTATAAACATAATTATCGTTTGGGAAATATTTATAAGCTAATAAAGTTTTGATTAATAAAGGTTTATAGCCATTTTTTTATGATCATTAAATCATCAGGTTTTTTAGATTTAAGTTCTGCTAGTTCCATGACAGGTCTAAATTTTTCACTTTCTGTAGAACTTTAATGGTTATCACTTGTTGTATAACTTTTTGATTTAACCTCTTTTGTTCCAAGTGCTTTTGAAATATCATTTAATGTTTGCTCATCATTAGAGGCTAAAAAATATACTACTTGGCAGGAATTTTTAATTACTTGAGCAGTTCCTTTCTCTTTCTTATTTATTTAAAAGAGCTAGAAAACATTAGATATGAAAGTAAATATGAAATTTTAAATGCAATGGACTTTGGGATACCTCAAAAAAGGAGAGGATATTTGTTGTTTCATGTCTTGGAGCAAATGAATTTTCTTTTAATAAATTTAAGAGAAAAGAAACTAGACTACTAATTGAATTTTTAGAAAAGGATGTAAGTGAACTTTATACAATGACCCAACCTTATATGTTGAAATTTTTAAATAAAGACATAGATAACAGTTTTAAAGGTCGACTAAAAGTGATTAAAGATTTTTTTATACTATTTCTACAAAACAGATGAGAGTACTTAATTTTGGAATAATAGATATTGGAAACGGTAAATATAGGTATTTAACAGAAAGAGAATGCCTAAGACTTATAGGTTTTGATGATAGCGATATAGATAAATTAGAGGAAGCACATCCAAGAAGAAAAAACTATATCTCAAGTAAACTATATAAGCAGACTGGAAATTCTATTATGGTTGATATTTTAACATGCATTATTATTTCAATAATGCATGTTAGTGCAAACTTATTATTGTAAATGATATAATATAACAAAAAATTGGAGTACATAATATGGATATTCTAAAATGTAAATTAAAGAATTGTTATGGAATAGACGAGTTTAACCATGAATTTGATTTTACAAATACAAATGTGATTACAATATATGCAAAAAATGGATTGATGAAAACTTCTTTTGCAAAAACTTTTAAAAAAATTCAAGATGGTAAAGCTGACGAAATACGTGATGAAATATTCGATATAAAAGCAGAAGTTAATGTGAGTATAGATGGACAGGATATAAGAAAAGAGCAAGTGTTTGTAATAAAAAGCTTTGAAAATTATTATGAGTCTAGTAGTGTAGCGGATTTATTAGTAGACGAAAAAATAAAAAAATCTATCACAACTTTATTAAATCAAAAAAATAATTTTTTAAAAAAGTTAGTGCAATATTCAGGAATGAAAATTGAAAAAACACAACAAGGAAGAAAAATTTATGAATTAGAGCCTACAATAGTTTCTGACATGAATTTAAGTGAAAAAAGTTTTTTATTAAGCTTGAAAGAATTAGATGAAGCTGAAAATGAAACGTATTTACCTAATGTTAAATACTCTACAATTTTTGATGATAGTGTAATTAAAAAAATAAAAGACGATAGTTTTCAAAAAAAAATAAAAGAATTTTGTGAAGCTGCTGAAACTATATATAGTAGTTATACTTTTTTTGAAAAAGGTAAATTCACATTTCCAAAATTGAAAAATGTTGCAAAAAAATTATATTCAGATAATTTTTTTGTAAGAGATAATAAACTTAATTTAAATAATAGCATAGTAATTAGTAATAATGATGAATTACAAGAGAAAATATCTGAAATAGAAGGTAAAATTAAAGATATTCCAGAATTTCAACAGATAGAAAACCTACTAAGTGATACAAAAGGTATATTATTAAGGGATACAATAGAAAATAACCCTGAACTAATAGAGTTTTTAAAAGAAGAAAATTTAAAGAATTTGAAAAAAGAATTATGAACTTCCTATATAAAGAAAGAAAATAAATCTTTTGATGATTTATTAGAGGCATATAAAGAATTAGATGATAATATTAAAAATCTCAATATCGATAATACATTGTGGAATCGCTCTTTAGAAATATTTGAAGACAGGTTTACTGTGCCATATAAGATGAAAATATCAAATTTAAAGGGAGCTATAATAGGAGAAAACCTACCACGTATGGAGTTTATATTTGAAAAGGGTGAAAATCACGAAAGTTTAGATAGATCAAATTTAGAAGAATTAGACGTATTAAGTCAAGGAGAGAAAAGATCTTTATATTTGTTAAATATAATATTTGACATAGAAAAAATAAAAAAAGAAAATAATGAAGTCCTATTTATAGTAGACGATATAGCAGATTCTTTTGATTATAAAAATAAGTATGCTATTGTAGAATATTTGTATGAAATGGCAACAGTTGACAATTTTAGAATGATAATTTTATCTCATAATTTTGATTTTTATAGAACAATTTCTTCTAGACTAAATATACCTCGTACATCTAGATTTATCGTTGAAAACAAACATGATTTGAATTTGATAGAAGAAAAATATCAAAAACAACCTTTTATAACATGAAAAAATAAACCAGATAAATATTCTGTATTTGCACTTATACCATTTGTTAGGAATCTAATTGAATATAGTTTCGATAAATGTGTTAATGATTCAAATTATTATGATAAAGATTATGTACTATTAACTAATTTACTACACAAAAAAGAAAATTCAGGAACTATTACTTTTAAAACTTTAGGGAAAGTATATAAAGAATATATAGGAAAATTTGATATACCAAATGAATTTCAAGAGGATGATCTGGTTATAGATAAGCTTTATGAAACCACTGAAAATTTAAGTATTAATTTAAACTTATTAGAGCATAAAGTTTTATTATCGATGGCTTGTAGACTTAAAGCTGAGGAAATAATGTTAGACAAAATTAACAATTACAAAGGGATGCTCAATTGGAAAGAGAAAAATAATTTAAAAACAGGAGATAAAGTAAAATTTCTAAATCATGTATATAGAAGTAATAATATGACCATGGGATTATTTGGTGGATATAAGCAGATTGCGAATGAAGAACAAATTAATATAGTTAATGAAGTTAATATAATGACTCCAGAGAATATTCATATAAACTCTTTCATGTATGAACCTATAATGGATATGGATATTAACGAATTATTGAATTTATATGCGAAACTTAAAGAGTTAATATAGGTTAGTTAATTATATTACTATATAGGCGGTAGACAATTTAAAATCTACCGTCTTTTTTGTTGATCTAAAAAGTAGGTAGAAAATGCAAGTAAATGATTTTAAGAATATACAAGAGGCTATAAAGTATGAAGTGTTACAAGATGAAAAAGAATATTTAAAACTCTTAAAAGCTATAGGTAATAATCAGAAATATGATTTTTCTAGCCAACTAAGTATATATAACAAGGAGCCTGAAGTTAGAGCTTGTGCAACTTTTGATATGTGGAAGAAATATTTTGGCAGAGTTGTTATGAGGGGGTGCAAAAGGGTATTCCAATTTTAGTTGGAAGTGATGTAAATCAAAGAATTTTATATATTTTTGATATTAGTCAGACCACATCAATGAATAAGAATATTAATGAGGTTAGTTTATGTCAGTTTGACCACGAAAACCATAACGAGGCTTTAAAAGAAATAATAAGAGATTTTTCATTTTAAGCTAGTGATTCACTTAATGAAAATATATTTTCTTTGAGTCAAATTTATGGAAATGAATATATTAATTTGGCTCTTGCTGACTTGAGAATATATACATACATACATATATATATATATATATTATAGAGTAGACTTAGTTTTTAAAAGTTTATTAGAGACTCAATATCCTATTCGATAGCTAATAAGTTTAATACGGTCTATTCTATGGATATAGAGAATTTAAAAAATAATTTTTCTAGGATTAATACAATTTCTTTAGAACAGATAGGTCTTGTAATATCAAGGGTTAGCGAAGAGATTATAGATAGCACAATAGAAAAATCTAAAGAAATAGATCGTGCTAGGTTGCTTACAGAAAGGGCCACTGCCGACTATAATAGATATATAGAAAATACAAATGAAGATAGAGGAGGTTAAAATGATTTATATAGACGAGATGATAGGTCAAGAAGTAGAAATGGACGAGTTTTTATAGATGGAAGCGACAAAAGAAATAGCTATGAAGATCGAAGAAAAAACCTTGGACATGATGGGGAATGATCTGGAGTTTATGGAGAAATTTCCAAATCCGACTTATGCAGTTCTAAGACTGTCTTACCTAGTAGGGAGTAAGAGTATGGAGAATTGGAAGAAACTTCAGTAAAAGTATGAGGAAAAAACACTTTTGAACCATCTGAAGGAAATTTAGAATTAGGCATTGGTCTTTATCAAGAGAGAAAAAGTCAAGATGATGAAAGCACAAGGATTGACATAGAAGATGATGAGAGAGAATCCAAAAGAATGCCAAGGATAGATGAACAATTTGATGTCAACAGTGAAGAAAATGGCAATCAAGGAATACATGGAAGCTTAGAAAATGAAATAAGCCAAGAAAAGGAAGTTGATGAAGCTTTTTTTTGATGGCAAAAACATTGAAAATAGAAAAGATTATTGGATTGTAGAATTTAATGAAAACCACGAATTAGTTCTTGATTATAGTGGACGGATAGTAACTAAAGATTTGATTAATGTCCTAAGACAAAAGGATATAGATGTTAAAGTCCATAATCAAACTCATGGAGAAAATGAATTTGGAAAAATGACCGATGATTATATCGAATATTTCTAATTCTATTTTGACCACTATGTAGACAGCGAAGTTGTCGAACATTATAGAATCGACCTTGGTGATGTTGAAGAAGTAAATGAGCGTGAATTTTCATATTTAGAAGAACAAGTTGCACTAAGTGAGAAAAAATTTTCACAAGAAAAAATTAAGGAAAAGATAGAACCTAAATTTAAGATAGACGATCAGGTTAGATATAAAAATAAGGACTTTACTCATTACAGATTTTGGTGAATTAAGTGGAGGATTTAAAACTATCACTATCATAGATAATATGGAATATATGGGAGGTATGATTAGAGGTTCGGAAGTAATTCCATATAGAAACGATTCATACCTTGAAGAAATCTTTGAAAATTTAAGTCAAACATCAGAAAAACTGGCAGTAAAAGATTATGATCTTAAAAAAAAAAGAACAAAAACACTATTTTGATTCCTTTACAGAATATAAATTTGCTGATCGTTTTTTTATCATTATCATAAGATAATGATAAAATTAAAAATAATGTTAAACTATGAACTAAAACCCTGTTTTTAATGGGTTGAAATACCAATATTTCAATGAAACAGGTCAAATAAGTAATTCATACCCTGATTTTATTCTAAAAATAAAAATACATGATCAAAAAGATCATTATTTATATATTGAAGTAAAAAAAATTAGAAAATGATTATGATTATCAAAAAACAAAGTTATTGATTGAATCGTACCAAAGTTACATTAAAAATCACAAACATTATTTAAAGATAAAAGAATTAGATGATGCTGAAATTACAATTTTAGTATGTTATATTAATTTAAATGAACAAAAAGATTTTTATTTTTATGGTGCTAGTTCAAATTCTACTTTAGATGAAAAAATTAATTTTGATATTTTAAAAAATACAAATAATGATAAGACTTTAAAATCACTAATTTCAAATAAAGCTAAATTTATTACTGATATTAAACAATTATTAGATTTATTAATTTTATAATACTAAAAATTTACTTATTAATAAGAATTTATCACAATTTAAAAACCTCTTATTAATTAGACCAAATATCTATAATGAAAGGTATTTATTAATTAGTATTTTAAAAAAAATACATTTGCTAGTATTACATTTATATAAATTATCATAAGGATTGAATCACTTTAGAATTTTTACTAAGGTTAATCATATATTTAGCAACAACCGTTGAATTTTCGTGTTCTGCTAATAGTATTTTGTTTTCCATAATATAAAAACAAACTCCTTTTAATATTTTTTAAAATCTAATAATTTATTGCGATAATATTCATATTGTTTTTTGCGTTGTTTGATTTCAAAAGGCAATCCTACGCCAATATCTTTTGTATATGCTTCTAATTTATCTAGAATTTCTACAATTTTATTTTGGATTGAAATGTGAGGGATAGGAATAGTAAAATTTTCTAAAATTGGGAGCTTTATATAAGAAATAACAGAATTTTTTTGGTTTGTTGAAAAATAATTTTTTAGAGAATTTTTAAGCAAATGATATACAAATTTGATGTTTATATTTTCATTTGGTTCCATCAAAACATATGTTCTTTGATAAGCATCAAATTTACCTTTAAAATAGTTAACATTTCCTAAATCTCCATTTCCAGGCACTAATAAAGCTTCGCCATCAAAGGAATATGTGTCATTTTTTTAGGTAATTTATTACAAGTTCAGAATATATAATTTCCATTCTTTTTTTCAGCGTTAGCATTTTTATTACCTGTTTTAATAAATTTAAAAATTTGTTTAATGTTTAATCCATTAATATAAAAAAATAATTTTTGATATATATCATTTAATATTCAGATGTAATCCTTACTTAGCTCTCTCTCGTGCTATATTATCAAAGTCTAATAACTTGTTACGATAATATTCGTATTGTTTTTTGCGTTGTTCAATTTCAAGAGGAAGTCCAGTATTAATATCTTTCGTATATGTTTCTAATTTATCTAAAACCTCAACAATTTTATTTTGGATTGAAATGGGCGGAATAGGGATTATAAAATCATCAAATATTTTATTTTCAAACTTTGGATATGTTGAGGTTTCAATATAAAATTTTTCATTATGTAAAACTAAAAAATAATATAAGAATTTATTTAGTAAAAAAGACGAATCTTTTGATGTTATAGTAATATTATTTGAACTAACAAAATATCCATTTCAGTATTTTATATTAGCATATCTTGCTCTACCAAAAGTTATAACTTCTCCTTTATTTAAATAAGTATTATTAACATTTTTAATATTACAAAATCAATCTCAATTTTTAGAACTTGGTAATAATTTAACTTCGCCAAATCCTTCATTTAAAGAAAATAATGAATCTGCATCAATTTGTTTGAAAGAGCTTTTTAATACTACTTCTGACATTTTTTTAAACTCAATTTTACTATCAATAGATTCAATATCATCACTGCACATTATTTTCTTTAATAATGCTTGATTTTTATTAAAATCTAATAATTCATTGCGATAATATATATACTGCTTATTTCTAAGTTCTAATTCTGTTTCTAATTCTGTTTCTAATTCTGTATATTTATCTAAAATCTCAACAATTTTATTTTGGATTGAAATGGGCGGAATAGGGATTGCAAGATTTAAAAATTCTTTTTTATTTAAATTTTTTAATGTACTACCTATAAACAATTTTTTATTAATAAAATTAGAAATATTTAATAGATAGAAGTAAAGATATTTTGTGTTATATTCATTTACTTTGAATGCGAATATATGATCAGAATACATTGTTTTTCCAAAATTATATTTTATATCTGCTTGACCGCCATCATTAACAAATATATATTCTCCATCAACTAAAAAATTGTCAACATAAAATGATTTCTTCCCTGATGTTATTGTTAAATAATTTCCCTTTTCCATTTTTAAAAGTTTTGTAACTCCAAAAGGTGATTTAGGAGCCACTGTAATTATATTTTTTAATTTTTTAAACTCCACTCCATTAGGGCAGAACTCATTAATTAATTTTTCAATTTTATTCATAATTTATTCTTCTAATTCTAAAACAATTTCATTCAATGTTAATTCTATTTATCTATGCATTATATATTTTCTATAAGAAACTCTTACATCTTCTATTGAAACTAAAGTATTTTTTCTTTCAATATGCCAATAACTAAAAGTATTAACTCTACTATTTTTATTTATCATTTTTTGGACTAATTTTGCTTTTTGTCCATTTGAATCTATCAAATATTCACTCAATTAAAATATCTATCTTTAATCATTTTATTCATATATACTTTTAAATGTTTTTGATCAAAGACAACATTATTAATGTCTTCAAAATTCTCTTAAGTTCTAAACATTTAACTCGATATAATTTTTTATTTTCATGAATTTACAAAAATATTGCTACATAATATTAATTATTTCCCCAGTTAAAATTTTATTCTTAAAATCCATTAACTAATATAACCTTTCAATTTAAAATTTAACAGTATTTTTTAATGTATTAAAAATATATAATACTATATTATCTATTCTATCAATTTAAAAGAGGTTTCTTAACTAAAACCCAATAAATAAGCAATATTTTGTTTTATTTAGAATTAGTATTTCATTAAAATAGTTAGTAAATCAATAATCTTAAAAGGATAAAGTTTATTATGATGAATCTAATTTATAATAAAACTAAGCGAGATTTTAATTTAATAATGCTAAAATATTTAAACGTCTGTTGTTTGGAGTTTATAAAAAATGAAATATAAAAAATATCAATCAATTTGGTTAAAAATAAGTGCTGTTATTGCGATTCCCTTATTAATAACAACGATTACAGCATGTGCGAGGATTGATAAGAAACAAAAAAGCAAAGAAGAATTACAATCAAGTTTAAATCAAGCTGTTTTATTAAAAGAAATTGTTGATCAATCATCATATGAAGAATTTAAAAAAAGATATAACCAAACACTTAAATTAAATTTAGCAAATATTGATCAATTATCAGAATCAAAATTAAACCAACAAAAAACAATAATTAACAACTTAATATCACAAGTTGATATATTATTTGAATTAGATGCTATCAAAAGGATTAATAATTTGATAGATCTTAATGGATTAGAAAATATTAAAAAACAATTAGAAGATTTTTTGAACCAATATGAAAAACTACTTTACCAAACAAATGTTGATATGACAATAATAAATAGTTTAAAAACAGAAGCTAATCAAAAAAAATTAGATACTAAAGCAATAATGCAAAAAACTATTTTAGATTTAGTTCATCAAATCAAAAATTTAATAAAGAAATCAGGATCTACTAAAAAGATTGATGAACTCGAAGCTGATATTCAAAATTTAATTAGTGATACTCCAAAAGATGATCATTTAAAATTTTATAAAGATTTTAAAATCATTTATAAGAAGTTAAATGATTTTAATTATTTGATCAACAAAGAAATCACGGCCCAACCTTTTATTAAAAAATTAGACGATATAGTTAATGTTATTAATACTATTTATGAACAAAATCATCATTTTCTATGATTTGATCAAACTAAATTAACAAATTTAAGACAAATTTTAATAAAGAATATAAGTCATGTGTTAAATGTTAAAAAAACATTAGAGATCAAAGAAATCAAAGCTTTAACTAATCAACTTTTATTTGACCTTCAAAATTACTTAGAAGGTTTAAAAGCGATTGCGTTAGAAAGTGAAAATAGTATTAATAAATTAATTAGTATTATTGAATCATTACAACCAGATGATTATAATCCCTACTATAGTATTGAAAAACAAGAAAATAAGCAAAAAAATGAAAACAATTCTCAAGCAACATATAATGGTTTAGAACACCAAATTAAAAGTTTAAAATCAATCATTAACTTATTTGATTTTAACATTACTAATTTATCAATTGATGCAATCTATAATCTTAATGATGAATTAATTAAAAAATTTCAAAAAGCTAATTCACAAATTAATGATGTTGTGATTAAAATTAAACAAACCATTTTATCAAATTTTAAACAAATTTATTTATTTAAGGACTTCATTAAACATTTAAAAGAGTTTAATATTGAATTAAATAATGAAAACGATCCAATAAAAAAAGCTAATCGAATTCAAACCATTCAAGAAGTCTTAAAAAGTTATGAAAAGGATTTATTTTTAAATACTAAAATTAATTTAAAACTTGCTCCAAACGAAGTATTAACAGTAAATAATCTTGAAAAAATTGATTTAACATATGCTTCTGATTCGCTGCAAAAACCACAAGAATTAATCGCTTATTTAGAATTAAATAATAATCAAGAAAGAGAATTTAGCAAAGAACTTGAAAATCTTAGTGTTAGAGTTTTTACTATCATTGATAAAAAACTTTATATTCCCAAAGCTAAAGAATTGATTGATGAAAGCAAAAAAATTATTCAAACAAAAGATGAGCACGCAAATAATAAAACAAAATTACAACGATTAGAAAAATTCACTAGTTTTGTTGAACAAGGAATAAGTAATGAAAATGTTTCTTCTTATACTCTTAATCTTTATATTCAAAGATTAACAAAACTAATTCAAGAAATTAAAAAACCACAGCATTAAAATGCTAAATAATATCAAAACTCCTCTAAAGAACTTTTTCTAGTAGAGGAGTTTTGATATCTTACTATATTAATTAATTAAAAATTAATGATTACGAAGCGTAAATATATCATTTTTGAGGATAATTTTTATCGATAATAACATTGTTATCATCATTAAAAGGTTTAATTACCTTATGTGGTTTTGTGCTTTGTTGTTCATTTGAGTTAGCAAGAAATTTAATTTCTTTTAATGTGTATGCAGTGTTTGGTGTTAAGTTATCAAAATGGCATTTTAATATGCCAATATTACCATCTTGATAATCTAATTTAACATCAGAAAGCAAAAATTCCTTAGAAATTTCGGTTTGGGGTGTGTTTACTGACGCTAGTGTAACACGATATTTTTGCTTATTTTCAAATGCATAGTCTCTTGAACCTAAATGAAGTTCAAAATCTACATTATTATTATTTCTTTCTTTTATATCCATATATTTTACAGAAGTTTGAGCTGGTTTAAGTTTGAACTTAGATATTATCTTGTTTTGAGAATGTCAAATTTCATATTTATTACTATTAGGACTAGTTTCAATTTCAGCATGATCAAAAGTATATTCGCGATTGTTAGGAATGTTTCATAAAGAAAAATGATAACTTGTGTCTGTTGCAATTAATTTAATACCATCAGTTTCATAAGCAACATTGTTATTGTCACGATATATGAATTTAACTTTTCGGTTAGCAAAATCATCATTAACCTTATTTGTTGTTATTGAAAAATTTACATTTCCTTCCATTTCATTATTAATTACACCATTAGCATCACAATTTTCAAATTTTATTGATTGTAAAACTGTTGGTGCATTTTTCATTGTAAATTCATATTCTTTAGTATCATTGGCTTTTCATTCATAAATTACTTTATTATCATTCGCTTGCGCATTTCCATATTGTAAATTATCGACAAATGCAATTTTATAAAGATAGTATTGGGTCCCTGCTTTAAATCCACTTAAGTTATTTAATTGATTAATAATCGCTTCTGATCCATCACTATTAACCTCCCAAGTTCCCCGTGTTCATTTAAGTGTATCATTTGAATCTGTATGGGATTTAAATCACACAATAACCTTAACATTATTATCAAGCGTTTTATCTTCTGAAAGAATTTTAATTTTTAATGCTTTTAGAGTTGTTTGACTAATTTGATCACTAGTTGGTTGTGTTCATTCAAATGTTGTTTTTCCAGGGCTAACTACAAATGAATCTGTTAAGTTTATCTTTTTATTTAAATCCACATAGTCGTTTAAATTATTGCGTTCTTGAATTAAAACTTTCTTAAAAGTATATTTTCGGTTAGGAACGCTGATTGGTAAATTTATTTGATAAACATTTCGGTCTTTTTTAAGAGTAATTAAATCAGATTCAACTGCTTTGATATCACCCCGATAATTATATTCATAAACCAATTTAACATTAAGATTATCATAATTAGCACTTACATGAGAAAAAGTTGCTTGAATTGTTTGGTGATTGTTTTTACCATCGTTAGTAGAATCTCATTTTAAAACCGCACTTGTGGCTTTTAAATCATGTAAGATTAAATTAGGTAATTGCGTTTTAATATCCACTTGTGGAAGTGATTGAATTTTTAAATTAGTATATGCTTTATTTGGTTTTGCTATTGTTATTGATTTTAAATGGTAAGTGGTTTGTGGTTTTAAATTATTTAAATTAAAATCAATCACCCAATCATTGTTAACTTTTTTTAGTTTTGTAGTGTATGATTGTAAATCATGAGGATCATTAGATTCATAAACAAGTGTAGCACTTAAATCCTCACTTAAAATATCACCATCATTACAATTAATTTTAAAAATAGCCTTTGATCCATTAGTAATTGAATCTTGATTTCAATTACCATATTTTACAATCGTACTATCATTAGGGGCAACAATAATTTCGGATTTAACATTTGGTTTGATCATTACAACGTTATGATCATCTAAAGGTGCTGTTTTTGAGGTCAAATAATAAATTGTTTTTAATTTATATTTACGATTAGTTTGTAAATTACCTAAACTAAATTGGATTTTATTAGCAACTATATTATTTGCACTTAAAACATCACTATAAATTGCTTCTGATGCGTCATAAATATTTTCATTACCTTGATTGTCAATAGGGACATATTTTAGTTTAAAAAATAAATTTTTATAAATATGTTCATTGGTATTAAAGTCAAGGGTTAAATTAATTGCATTTACCTTTTTAGCATCAGATGCATTAATACTTTTTAAACTTTCATGACTTGATGCTAATGGCCCATTAATTTCAAAATTATTATTAATTACCAAAGGAATTGCTTTTTCATGTGTATTTTGATAAATAATGTTATTTGTTTTATCTAACCCAATATTTTCAATAGCACATGTTGGTTTTTGTTCAAATGTAATGGATTTAATTATTGTAGGCGTATTTAATGTTAATTTAGTTAAGGTTGCTATTAGATACTTAACACCATTGTTAACAATAACAGTTGCTTCACTCTTTTGATTATTACCATAGTTAATAATCGCTTTTTGATTAGCTAATAAAACATTATCCACATCTTTTAAAGTGATTTTAACTTGTGCATTCGTTAAAGCAGTTCCCAAACGATTACTTATTTCACTAATACTTGTTGCTAATACAGCACTAGTTCTTTCAACCATAAAACTATTAGTTAAGTTAGTATCTAATGGTAAATTAGTTTTACCATTGTTATTTATTAATTCAACCGCCTTTAGTGTATATAAACGATTTGCTTTTAAATTATTTAGTTTTAATACATAGTTTGTTTGATTTTTTTCTAAAACTAAAGGTTGAGTTTGAACATCTGAACCATCTGAACTTGAATAAATAACTTTAATTTGCTTATTAACTCAGTCATTTTTTATGTTAGATAGTTTTAAATTGATTTCTCTTTTAGTTTTATCAGCATCATTTTCTAGAGCACTAATACTAATAACTTTATATTCTTTTAAGAAGATATCTTGTTTTTGATTACTTACTAAATCAAAATTATGTATTAAAATTTGATTTTGATTTTTTGCTAAAACAACTTTTTGTAAATGATATTTTTTATTGGGATTTTTAGTTAAAACTTGAGTATCAAACATTATTGTTCCATCATTTTTATTTACAGCAGGAATTAAATGTTTTTGGTTATTTTGGTCTTCAAAAACACCATAGACTTGTTCATTAGCATAGCGTAATGGTATCTTAGCAATTAAGTTAAGATCACCATTTTTGCTATATTGGTATGGTGTATTTAATTCATCAATATTTAAATTATTTATGATCTTATTGCTGTATTCAATTTCATTAACATTAACGATTTTAGTTTGGTCTTGTGTTTGTAAAGCAATTACCTCATATAAATTATTATTTTTTAATTGTGAAACATCAAAACTAACTTCTCCCATATCATTTGATTTGGCTGTAATTTTATATACGTTATGCTCTAAGTCTTTTAGTGTTAATTGAATTAAAGTGTTTTTTAATAACGTGTTTAGTTTAACTTGATAAACTTTTTTATTATTAATAAAGTTAACTTTTGCATCAACTGGCTTACTAAAACAGATTTTTTGCTGACTAACTAAAGCATCATTTGTTAATAAAACATGGTTATAGTCATTAGTACTTATAACTTTTTTTAGTTCGTAACTATGGTTTAGTGGTAATAATTTGATGTTAAATTCAAATTCTTTTTTATCATTAACAAGTGCTTGAATTTTATGTTCTTGATTGCTTTCATCACTAAAAATTCCAAAAACATAATTAGTATTATCATGTGGTTTTAAATAACCTTTAATAATTAAATTGCCGTTTTTATCAAATGAATAATATTTTTTTAAGATAGTATGTTTTGAGTAATCTTTGCTTTTTAGTCTTAAAACAATTCCTGTAATAACGCCTGCTCACACTAAATTTAAAGTAGCTAGCAAAATAAACATATGGTGTTTATATATTTTATTAACTCTTTTTTTCATAATTATTTCATTCACTTTCATATATATATATATATATCATTAAGAATTTAAGGATTAAACTAGTCTTTTAAAGTTGTAAAGTCATAAGGATCATTTATTTGTAGATTCAAACTATAAATAACATTATCATTATTAAATGGTCTAGCAGCTCTAGTAGGTTTAGCACTAATTTCTTCATTTGGATTAGCAATAAATCGAACTTCTTTTAATAGGTATTTAGTTCCTTTTTTTAGTTTGTTTTCAAAATTACAGCTCAAAGTTGCTATATCTTTGTTCTTATCATTATTATCAGCAATTGGGCTATCATTTTTATTAATAGAAGTGCCAGCAAGTAAAAAGTCACCAACAACAACATTGTTTTGATTTTCAGCTTCAACTAATGTCACTCGATATTTTTGCTTATCTTCAAATGCCCGATCTTTTGATTGTAATTGAATATCTAAAATTGCCCCATTATTAGTTATTTTTATAGGATGTTTTCACTGTATTTCTGTTTTACTTGGTGGTGTATAAAACTTGAAAGTTAATCCTCTTTTAATTTTTAAATCTTCTATTACACCACCATCATAAGGATAATTAGGATCTGTTGTTTGTATTTCAGCATTATCAAAAACATATTCGCGATTTCATTTTAAATCATGAATTCAAGAATGATATATAAGATCATCAAAACCTATTTCTATATTATCAGAAGTATATAATTCATTGTTATTATCACGACAATATATGGTAATTTGTTTGATTGAACTAGGATCATCATTAGCTTTATTTAGATAAAAATAAAAATTTTGTCCTCCAACGCCACCATTATCATGTTTTTCTATTTTTTCTAAAATTAAAGGGGCAGTTTTAGTTCTGAATGTATAATTACGTGCAATATTTTTATTATATTCATAAACAATGTTATTACGATTTGGTGGTAATTTTGAATATTCTAATTCATCTATAAACTGAATTTTATAGATGTAATACTCTGTTTGCTCTTTCATACCGTATAAATTATTTAAGTTATTAATGACAGCAGCTGTTCCATTGGCGTTAGTTTGAGATAATTGACCTATAAATTTAAGTGAATCATTATTTGGTTCGGCATCTGATTTAAATCAAACCATAACTTTTTTACCATTTTGTAATGCATAATCTTCAGAAGTAATTTTAATATTTAGTTTTTTTAAACCATCATATGTAATTTGACCATCATTCGGTTTTTTTCATACAAAATCTGTTTTACCAGGACCAACAATAAAAAAAGATTTTAAATTTGGATCATGATCTAAATCAATAGATTTATTTGGTTGATTTAATTTTTGAATTACTACTTTTTTAAAATGATATAAGCGATTTGGAATACTAATTGGTAAAAGAATATTGTTATATGTTGTTTTGTTTTTTTCTAATTTGAAAGGTTGTGATGCAATTTCTTTCATTTCTCCACGATAATTGTATTCATAAATTAATTTAACAATATAATCATCAAAATCGTCATTTATATTTTTAAAAGTAGCAGTTATGAATTGGTTTTGGTTTGTATTATTTCAAAGAATTAAATCTTTAGCAACTTTTAGTTGTGTTAAGTTAATTCTTTTAATTGGATTTTTATCAACGATTTTATTTTTTAATGGAATTTCATTAATATTAGCAACTTTTTTGTTATTAGCTTCAATTTTTGTTAGTGTATATTTATGATCTGGTGCAAAAATTGTATTATCACTTGTTTTAAAAATAGCCTTTCCATCTAATCCAATGATAGCTTCAATGGTTTTTGTATGATCATCTTGATCATCAACTTTGAATGTTAATTTAACTTTCTTATTAACTAAATTAGGATTCGTAAAATGCATTATATAATCATTGTTATCATTAATTTGAGCAACCACTGTTGGTTTTTTAAATGAAATATGATTATCACCACTTAATTGCTCTTCACTAATTAGATTAATATTTGGTTGATCATTTTGTTTTATAACAACTTTTTTAAGTGTGTATATATTAGGTTTAGTTGGGTCATTATTTACTAAATCTTTTGTTGGAATAATAACCTTACCATTAGCATCAATACTTACATCTATCTCATGTTCTACACCATTTTGATCGACAACAATTCCTTTGACATTTGCATTATTTAAAGCTTTTTGTTTTAAATCATCATTTAAATCATTTGCTAATTGAGTGTGAATTTCTAAATTTCCATCATTATCATACTCTCAAGTTGGACGAGCAATTTTATGGTCATCTTTACCAAATTGTTTATTAACATTTTTTAAACGCAGAACAATAGCTATAATAACGCCTGCTCATATTAAATTTAAAGAACACAACATAAAAAAAATGCGATATTTCCGTTTTTTTATGTTCAAAAATTTGTAATTTTTATTATTTGATTTCATATAATTTTTCCCACATTTTTATAAAATAAACAGATAAAAACAAAACATTTGTTTACTCATATTTTTTCAAAAAAAATACAATAATGTTTTAATAAAATAACGCAAACATTTTAACATAAAAACTAAAAATTTACACAAAACAAAACCCATAATAATTGAAATTATTATTAAAGTGATTTTATTTATTTTTTATTTTCATAAAATAAATAACATAATAAATACTTAATTTTTACAAATAAAAATCTTGCTTAAAACCTACTGGTGTGTTTTAGCAAGATTTCTTAAATTAATATTTTTTACTTTTTATTTACTTTCATCAAAGTATAAAGCAACAAGTGAATGATTATCATCAATAATGCAATCACCATCAACTAACAAGTCAACAAATGCAGATAAATCAACAGTATATTCTTTGCCATTTTCTGCAACTAAACTAACTTCACCCTTACGTAATTCATCTTTTGAAGTTAGAATTTTATGTAAATGGTAATGTTTTCCTTCTGGTAAGATTAATGGATGCTTGATAATATTACGAACAATTTCTTTATAAATCATAAATAAACTACTCCTTATTTAACTAACTTTTTATATACGTAAATTATAAAACAATTTAAAAAATAATAGTAGATTTTGTAATTTTAATATTTTTTACAAAAATAAATTAGTAGATCTCTAAAATTGCTTCAATAATGGATGGTAAATGCTCATTAAATCATTTATCAATTAGTGATAAGTTTTTATTAAACTGTTCTTGATTGGAATTGGGAAGAGTAATATGATCACTAACTAATTTAATACTTACTAGTGGTGTTAACATACGCGTGCATACATGAGCAATAGCTGCTAATTCCATATCAACTAAGTCGATTTGTTGATCATAAAAAAATGAGATGTTATCACGATTAATAAATGTGTCACTGCTTCCAACATTTGCAATTCAACATTTTAGTTTTTTCTTAATTAACTGGTTTTTGATTTGTTGATTATAATTTTTATCTAAAATAAAAAATTTATCTAAACGCGGTATTTGATTTTTTAAATAACCAAAAGCGCTAACGTTAACATCTAAGTATTGGCATTGATCAATAATGGCAATATCTAAAACCTGTAACTGATCATTTAGTGCACCACAACTACCAACATTTAAAATCACTTTAGGTTTAAAATTATTAATTAATAGTGCAGTAATAAAACTCGCATTAGTTTTTCCTACATCCGTAAAAACTAAAACAAATTCTATATTTTGATGAGTGCACAAGTAAAAATCAATATTATTAATTTGATAAACCTTCTTTTTAATTTGTTTAAAAAAGGTTTTAATTTCACTATTTAATGCCACAATTAAACCGATCATATTTCTACCTTATTTTCTTTGTCTTTGTTGGTATTGATAAACTTCAATAAATGCCTTATCTAGATCTTCAAAAACTAATTTTCGATAATAATCAATGTTAATTTGATCAATTTCTTCCCTACGGTTAGGCTCTAATTTATCAGCACAATATAGAATTTTATCTAAAGTAGTTAATTCACTATAATCTTTAATAAAATCAAAAGGTCTTGTATGACGACGAATAGCATTTAAAATTAATTGATTTTTAAAACCATATTGGGTTTGTAATAAATGTGCACCAAGATATGAATGTAAGATTCTTCAAGAGATAAAATCATCACCAATATTTAATTCACTTTTATGTTCATTAAAATATGCAACACTTTCTTGTTCATCTAAACATTTTAGTATGTCATGATAAATTCCCGCACAATAAGCTTGATCAATTAGTTTTTTATCATAGGCATTCATTAAATCAACACACATTTGAGCAACGCGGCGACAATGATTGATGCGTGCAACTTTTTTTTCTAAATTATTAAAATTATCATATGAAATTAAATGTTTTTCTAATAATCATAAAGCATATAAACCATGTTCATTAATATAATCAAGGACTGCTGGATCTATTCCTGATGTTAAATTATGGCGTTTTTTAATTTCAGTAGAACTGATATTGTAATTAAAATCATTAACCAAAAGAATTTCATGTTCGAAAAAATTAAAACTTTCGCATTCACAGCTTTTTTGTGGGCAACTTTTTTTAACACAAACATCCCCACGTTTAAAACAAATAATTTTAACTTCTTTTAGAATTCGTTCCCAATCTTTTCATTGTTTAAAACGTTCTAAGTTATCACTACCCATAATAAAATAATATTCATGTTCACTACCATATATTTGTTTAAAATGCTCAATAGTATTGATTGTATAAGATTTATTAATTTGTTTTAATTCGTAGTCACTTACTTCAATATTTACTAAATTTTTGGTTGCAATTTTTAACATGGCTATACGATGATGTCCAGGTGTTAATTTTGTTTTTTCATACTCTAAATTGTCATTTTTATTAATTGCTTTAAAAAATTTAAAATTTGATGGTAAAAAAATTAGTTTGTCAGCATTAACTAACTTCATGGCTTGTTTAGCCATTGCAATATGAGCATTGTGAACCATATCAAAAGCTCCACAAAACAAAATGATTTTCATAACAAATCTCTCTTTATTAATAATAATTAAATAATCGCATATTTTGCTACTTTTAGACCAACACTTTTTGGTAAACGGATACATATTTGTTCAGCATGAGCATTAATAACTAACAAACCTAGGCCTGCAATACAAATATTGGTTTTTTGATCCTTATTTAAAGTTATTAGATGGTCAACAAATTCGAGATCTTGATTTAGATAATTGAACTTCGCTAATAAATATCGTTTATTAAAATTCATTATAAAATTTTCTAATTTACAACGATGAATATCTAATTGGAATGAAACATATAATGTAAAAGTTGTTTTTTGACCCTTTAAATACTGAATTCCAACCAATCCAGAAATCATAAGAGCTTGTTGGGGATTAATTTGGTAATTAATTGCTTTAGCACCATAAGAAATCATGATTGATTTATTATCTTCATCACGTACATAATTTAAAATGTTTTCATCAAAACAAACTCCGGGAGCATCAAGTATTTGATGTTCTTGTATATTAATTTTATTTAAATTAATTGTTGTATTAGTATAACTACTAATTGTAAGTTTAGTTTTAATTTTATTTAGCGCTAATAAAGCATTGATTAAACTTGATTTACCAACATTTGATTTTCCAAAAAAAATGGATTTTTGTTTTTTATTAGTTGCATTTTTAATATAATCCATAACTTTTTTTAAACTGGTATTAGAATGTGTGGAAACATAGATAATTTTAGGATCAATAAATCCATGATTAATAAGTGTTTTAACAACATTTTCATTAACTAATTCACTATTGTAGCGCTGAGGTAATAAATCAATTTTATTAACTAAAAAAATAATTTTATTTTGATATTCTTTTAGTTCATCAATAATTGTGTGTTCTAAATCTAAAATATCTAATACAGCAAAAATCTGTAAATTACTAAAATTTAAAGTGTTAATTGTCTCTTTAATACTTTGATTTAAATTAGTACTTACTTTTTCAAGTTTATTATAGTGTATTAATTTAAAACAACGTTGGCATAAATTGGTTTTTGTTTCATTTATTTTTGGGGTATATCCAATTGTATTGATTTGATCATTCAAAAAAGCTCCACAGCCTTTACATTTTTTATTTGTTATCATTAATTCTTTTCTTCCTCTTCATCAATTAATTCTAATTGTGAATCATCGTGATTTTTATTAATTTTTAGCATTTCATTATCAAATTCATTTGCGTACATATTAATTTTTTTAAAATCACTAATTGCTTCATCACTTAAAAAGCAATCTTTAACTACATCAGTGATCATTTGATCGTTTTCATAGATACTAATTTTAGTATCTAGTTTTGTTGGTTTGGGGTTTGATAAGTAATATGGATTTATTTTTTGTTGTTGATCTAAAAGATAAAAAACATTTTCATGAGCAACTAAAAAAGCTGCTAAAACATTTTGCACTTTTTTTGTTATTGGATATAAATTTGTTGGTGTAGTTGCACGTTTTGATAATTTTAATTCACTAATATTAAAACACTTACTTCCCCGTTCTAAAAAAAGTTGAACATTATAACCATTATTAGGAGTAATTAAAATAGCACTAACCTCATCATTTGGTTTTAATTTCATAATTTTAATTCCTTTAGCTAAACGATTTAAGCAATTAATTTCATGAACTAAAAATAGCAGGGCTAATCCTAATTTAGAAATTGCAATAATAAATTGATTATGACCTTTTTTGATTAAATTAGCACTGATTAAATTATCATTATCATTTAATTTCATAATGCTAATTTGTTTACTATTTTTATTTATATCCAACTCGCTTAATTCAATTCTTTTAATTAGATTTAATCTAGAAGCCAAAACTAATTGATATTCATCAATTTTAAATTGTTCGTTCCATATATAAACAGCTATAACTTTTTCATTAGCATCAAATTTTACATAATTGTTTAAGTGTTCACCAACATCTTTTCACCGCATAGAAGTGATTTTATGAGCAACTAAATTAATTAATTTAGCTTTTGATGTAATGATTAAGATTTTATCATGAGAATTAATGATATTATGATAAAAAAGAATATCATTAGTTTTTAATCCTAATTCATCATATTTAGATGATTCAAATGACTTTTTAGAAACAGTTTTAATGTATCCATCACGACTTATTACTAAATTCAAATTTTGTTTTTCGATCATTTGATCTTCGTTTATTTCAACTTTTACTAATTCATCAAATAATTTTGTTTTGCGTTCGAAACCAAAAGTTTTTTTAAATTCAATCATTTTTTGTTTTAAATAATTCTTGCGAACTTGAAGTGAATTTATTCGTTCTTGTAGCGTTTTAGCTTCGTCTTGTAGCGATTGTCATTCATTTTTTAAATCAAATATATCAGTACGTGTCAAATTATGCAAACGCATCATAATAATCGCTTCAGCTTGATTATTTGTAAAATTAAAAACTTGAATTAAAGTATTTTTTGCGTCTTGGCGCGATTCACTAGCACGAATAATTTTAATAATTTCATCAATTAAATCAACGCATTTAATTAAGCCTTCAACAATTTCAATACGTAACAAAATTTTGTTTAGATCATATAAATCGACTTTATTAATTACATCTAAACTATGATTAATTTGAGCATCTAAATATGTAGATAGATTTACAAGTGTAGGAGTTCGGTGAACAATTGCAATAAATTTTGTATTATAGCTGATTTCCAAGTTGGTTTTTTTATATAAATAGTTTTTAACATTTTCTAAATTAATATCTTTTTTAGTATCAATAATTATACTAATTCCCTTAGCATCAGATTCATCACGAACTTCGATTAATCCAGCAACTTCTTTATTATAAATAATATCTTCAATTTCTTTAATGATTAAAGCCTTGTTTGTTTCATAAGGGATTGAACTAATAATAATTTGATTAATCTTATTATCATTGATTTTAAAATCAATGCAAGCACGAATTATAAATTTACCTTCACCAGTTTTGTGAGCATTAGCACATCCATTTAAATCGTGGATTTCCCCACCTGTAGGAAAATCTGGCGCAGGACAGATTTTAATTAGTTTTTCTACACTACAATCTGGATGATCAATACGATAAATTAAAGCATCAAAGACTTCATTAGGGTTATGAGGAGGAATATTTGTTGCATAACCAGAAGCAATTCCTGTCGCTCCATTGATTAATAAATTAGGAAAAAGAGATGGTAATATTGATGGCTCTTTTTCACTATCATCAAAATTTGGACTAAATTTAACAACATCTTTTTTTAAGTTAGCTAACATTACACTAGCAATTTTTGATAATCGGGCTTCTGTATAACGCATTGCAGCAGCATTATCACCATCAATTGAACCTTTATTACCATGCATATCTAATAAACATAGATTATTTTTTCAATCTTGACTCATTCTCACCATTGCTTCATAAATTGATGAATCTCCGTGTGGATGATATTTACCAATTACTTCTCCTACTGTACGTGCAGATTTTTTATAAGGTTTATCATAAAAGATTCCTAATTCACTCATTGCGTATAAAATTCGACGTTGAACAGGTTTTAAACCATCACGAATATCAGGTAAAGCACGATCTTGAATAATGTATTTTGCATATTTTGCATAACTTTCACCAACAATGTTGTCTAAAGGTGTATTAATAATTTTTTGATGATTATCACTCATATTTTTTATTTAGACTCCTCATTATTGATTTGCAATTCGTCAATGACACTAAAATCAATATTATTATCAATTCACTGCTTTCGAATACTTACGTCATTACCCATTAAAGTATTGATTTGCCGTTCGGCTTGTAAAATATCATTAATATTAATTTTTAATAATAAACGATTGTTTGGATCCATTGTTGTTTCAAATAATTGATCAGCATTCATTTCACCAAGACCTTTGTAACGTTGGATTTCATAATTTTTTTGTTCTTTTTTTAGTTGGTCTAATTCAACATCATCCCAAGCATAAAAGAATTTTTTGTTATTTTTATTTGTTAATTTATAAAGTGGAGGCAAAGCTATGTAAATATTACCATTTTCAATTAAAGGCCGCATAAAACGATAAAATAAAGTTAATAATAAAACTTGGATGTGACTACCGTCGGTATCTGCATCGGTCATAATAATAATTTTGTGATATTTTAATTTTTTTAAATCAAATTCTGCACCAATTCCTGTTCCTAAACAAGTAAAGATTGATGCTATTTCTTCATTTTTTAAAACATCAACTAAACGAGCTTTTAAAACATTAAGAACCTTACCTCTTAAGGGCAAAATAGCCTGGTATTTTTTATTTCGTCCTAGTTTAGCTGATCCACCAGCTGAATCACCTTCAACCAAAAATAATTCATTTAAAGTTGGATCTTTACTTTGTGCTGGTGTTAATTTTCCGCCCATTCCTCGTTCTTCTTTAATTTTTTTAACTTTTTTAGTTTCTTCACGAGCACGCTTTGCAGCCAATTTAGCATCACGTGCAAGAATTGCTCGATTAACTAAATCTAAAGCTTGTGTTTTATTTTCTTCTAATCAAAAACTAAAGTTATCTTCAATTGTTTTTTTTACTGCTTCATTTGCTTCTGGTGTGAATAATTTGTTTTTAGTTTGACCCTCATAGCTAATTAAGCTTTCGGGGATTCGAAGTGAAATTACACTTGATAAACCCTCACGTATATCATCACCTTCTAAATTTTTATCTTTTTCCTTAAGTAAATTATGTTTTCTTGCGTAATTATTAACAACACTTGTTAAAGCATTTTTAAAGGCATTTTCATGTACACCGCCTTCATTAGTTTTCACAGAATTAGCAAATGAAACCATAATTTCATTGTTTTGATCACTATATTGAAAGGCGATTTCGACTTCTATTTTGTTAATATTACTTCTAAAATAAATAACATCCGAAAAGGTCTTTTTAGCTTCATTAATAAACTTAATAAAATCAATTAAACCTTGATTATTAATAAATTCGTACTTTTTTTTATTAATTTTATCTTCAAAAATAATTTTTAAATCTTTTATTAAAAAAGTTGATTCATGTAGACGCTCTTTTATTATGTTAGGATTAAAAACTAAATTTTTAAAAATTAGAGGATCGGGTAAGAAATGAATTAATGTTCCTGTTTTTTTAGTTGTTCCAATTACTTTTAAAGATTGATCAATATGACCTCCATTAATAAATTTCGCTTGGTAAATTTTTTGATCACGATAAACTTCACAAATTAATCAAGAACTTAAAGCATTAACTACTGATGAACCAACACCATGTAAACCACCAGCAGTTTTGTATGCACTTTCATCAAACTTACCTCCAGCATGCAAAACTGTTAGAACAGTTTCAACAGTAGAAATTTTTGTTTGAGAATTAATGTCAACTGGTATACCACGCCCATCATCTAAAACACTAATTGAATTATCTTCATGCAAAATAACATTAATGTTTTTTGCGTTAGCATTCATAACTTCATCAATTGAATTATCTACAATTTCTCAAATTAAATGATGTAATCCAGTGCTACTTGTAGAGCCAATATACATTCCAGGACGCTTACGAACAGCTTCCAACCCTTCTAAGATTTTAATAGCATTCCCATCGTATTTATTTGCCATAAGATTTTATCTTCCTATCTTTGTTCAACTAGGATCGGCATAAAATGGTTGCTGTTGATTGATATTGTCATAATATAAACCAGCATCTAAATGACCAATTTCATGTTGGATGCAAATTGCAATAATACCACTAAATTCTTGATCAATAAATTGTTCGCTAATTAGATCATATGCTTTAACATGAACTTTTTTATTTCTAATAACATATCCAGAACGATCATCATCAACAGATAAACATCCTTCACCTGGATTTAAATAAGCTATTTCGGATGAACGTTTAATAATATGAGGATTAATTAATAAATAATGATGTTCTTTGGCTTCATCATTAAAATGGATATAAGTTGCGCGTTTGTTTCAACCAACTTGATTTGCAGCGATTGCAATTCCTGAACGAATTTTATATTTTTTGGCTTGATTATGATAACAAACATCAATATAACGGACCATTTTTTTAAGATAATAAATATCATCTTTTGATAATTCATTACCTTCTATATCTTGAGTTACTTCGCGTAAAACGGGATGTGGATCTTTAAAAATTCATTGAGGGTTAGGCTTTAAATTTGAATTTAATAAATCTAAAAATTTAATATTGTACATTTACTTACTTCCTATTTTTTGAAATGAAATCATAATTAATTGGTTTTTATTTTATTAACCATGTTTTAATAATAATTATAAATGATATAAAAGTTTATAATGTATGATTGTTACTAATTAAATAAATTATTTAAATTCTATTAACATTCTAATAAATAATTTTAATCTGTTAATTAATTATGAATGTTGCTATTAATTTCATATAATAATATAATGTGTTCATAAATTGATGTTTAGGTGGGGATTACATGCTTGATAACCAAGAAATAATAGAATCTATTTTACAAGCAGATAACGCAAAAGGTGGTAATGGCAACTTTTTAATGATACTAATCATTATCATTCCAATTGTATTAGTTGTAGTATTTATTATGCGTAAAAAACGTAAACAAAATGGTGAAAATCAAACTAGTGTTAATAACAAAGATAAGTCAGATACAAATGAAGTTTGAACTACAATTAAAAAATATTTACGTAGCATTGACGATAAAGGAAAAGAAGTTATTGATTCATATGTAGTTAAGCGTGCTGAACCACACAATTTAGCTCAGATGACAAAACAACAAAGAATTGATTATAAAAATGAACAAAAAGCAATCAAAACACTTAAAACAACGAATCCAGAGCAATATAAAATTGAAATGGAACGAATTAAAAAAGAAAAACGTGCTAAACCTAAAGAACTATATGTAGTTTTGTTTACTACAAGAAATGCTAAAACTTTAATTATAGATGAACCTCGTGCGATTGAGTGTGAAGTTCGTTTGGTAAAAATTAATAAAAAAGAAAACCGACGCGAAATTGATGTTGTGCGTGCTTTAGATTATGAAGAAGAAATGTTATGAATTGAACCAATTAAAGCTAAAGATGATGAGATTTATAACAAGAGATTAGAAGCAGATAAGAAAAAACAACAAAAAGCAGCTCAACGACGTCAAAAACAATTAGAAAAACAAAAAAAATAAAACAAACTAATAACTCCATTTTAAGGAGTTATTTTGCTATCTTTACCTTAATTATAAGGTATTAAAATAAGGAATTTAATTAAATGAAATATAAAGTAATGTTTTTTGGTACACCAGAAATCGCTAAAATTGTTTTGGAAACATTATTTAACATGCATGAAGTTGATTTAATCGCTGTTGTTAGTCAACCAGATGCACATTTTGATCGAAAAAAAAATGTCGTCTACTCTCCTGTTAAACAGTTTTGTTTAGATCATAATATTAAGTTATTTCAACCAGAAAAAATCAAAGAAATTGAAGAAGAAATCCGTATTTTAGGACCAGATATTATCATTACTTGTGCTTTTGGTCAATTTATTAATCAAGGGATAATTGATATTCCTAAATACAAAATTGTTAATATTCATGCTTCACTTTTACCAAAATTACGTGGCGGAGCACCAATTCATTATGCCATTTTAAATGGTGAATTAAAAACAGGAATCACTTTAATGCATACGATTAAAAAAATGGATGCAGGTAATATTTTATTTCAAAGAAGTTTAGAAATAAATGATTGTACAACAACTAAATCATTAACTTTAGAGTTAGCTAATCTTAGTGCGTTGATGATTAAAGAACATTTTTTAGAATTAGTAAAACCAAATTTAGTTGGAATTCAACAAGATGAAAATTCTGTTAGTTTTGCTTATAATATTCAAAAGGATCAAAATATTATTAATTTTGACCAACCAGCTTTTTTTATTAATCGTTTTGTTAATGCTATGTATGATAAACCAATCGCTATTATGCAATATAATGGCGTTTCAATTAAAGTATATCAAGTTAAAATCACTAATCAAAAGTCATGTCAAAAACCTGGAACAATCGTGATTTTTAAAAATCAACTTTTTGTAAGTACGCAAGATTTTGATATTGAACTATTATTGATTCAACTTCCAAATAAAAAACCTTTATCTCCAAAAGTTTTATTGAATGGGAGAAACCCTTTTATTAATTAAAATATAGTAAATTGTTATAATTTAATCATATTGATTACCAACCATTTATTAAAGAAAGGACAGTAAATGAATTTAAGGCATTCAAAAACTACTTGATTTAGAAAACCCTTTCAAACTAATACACGTTTTAAATCACCTCTTTTATATAAATTCTTAATTACATCATTTTTTGTTGTTATTCCAATGATTGTTTTTTTTATTTTATATGGTGAAATCAATCTTTTAGGTTTTAATGGAATAATTGGTGTTAATGGTCGTTGGTATGGAGAAGGTTTTAAATTAGATGAAAACCAACAATGAATTGTAGGAGTTAAAGATTTTTATCATTGAAATCCTATTGTATATGATCAATTAAAACCAAAACCTGATGCTTTTATGATAGAAAAAATGCAGGCATTAGTTAATAAAATTACTAATCAATACAATCATGGTATTGAATTTGGTACAAATATAATTAGTTCATATCCAGTTTGAGTTGCAATGTTTTCAACTTTAGGATTAGCAGTTATTGTATGTATTATATTTGCTTTTAGTACGCCAAAGATTCGTTGAGACATTCTTACGCCAGTAGTTTCATGTTGATTTGGAATGTTTATTTTAATTATTTCTGGATTTATTCCTAGTCAAATTTGAGGATATTTAGTACGATTTTTAATTTTAGTAGTTGCTTTTATTTTACCAATTTTTATCATGACAAAAATTACAAATGCTATTATGAATCGCTCAAAACATTTTGAGACATATGCTGCAGATTTATACAATGAACTTAAAGATTCAGAACAATATTATAATGAATTTAACAAAAAACGTTTAGAATTAAAAAAGCGTAATAAAGCGAATGAACGAATAAAATATAAAATTGAAGATGATCAAAAAGGAAAATAAGCATGGCATTTATTGATAAATGTAAAATTGTATTAATTGCAGGTAATGGTGGAGATGGAATTGTTTCATGACGTCGTGAAACTCATGTTCCAGAAGGTGGGCCAGCTGGTGGAAATGGTGGAAATGGTGGGTCAATCTGATTTGTTGGTAATCACAATGAAACTTCATTAGAATTTTTAAAATATAAAAAAATTATTCGTGCAAAACATGGTGAAAAAGGCGATATTAAAAATCAACATGGTGCGAATGCGGAAGATGTTTTTATTAATGTGCCACTAGGAACAGTTGTTTATAATCCTATCACTAATGAAATTTTGGCAGATATTAATATTGATCAACAAAAATATTTAGTTGCCCAAGGTGGTTTAGGCGGTCATGGAAATACACATTTTAAATCGCCTTTTAATAAAGCACCAAATTTATATGAATTAGGAGAATTAGGTGAAAATGTTGAAGTTTTATTAGAATTAAAAACTATTGCTGATATTGGAATTATAGGATTACCAAATGCAGGAAAATCAACATTAATTTCTACATTTACAAATGCTAAACCAAAAACGGCAAATTACATGTTTACAACTTTAAATCCCGTTTTAGGTACAATTTATCGTGATCAAAATCGTATCATCTTTGCGGATATTCCTGGTTTAATTGAAGGAGCACATACAGGAGTTGGCTTGGGGCATGATTTTTTAAAACATATCGAACGTTGTTTTTTATTAATTCATTTAATTTCTTTAGATCCTAATGATAATCCTGATATTATTAATGCTTATGAAACGATTGTTAATGAATTAAAACAATATAAACAAAATTTAGTTAACAAACCAATTGTATTGGTTGCTAATAAAATAGATCAAATTGGAGCATTAGAAAATTTGCAGATTCTAAAAGAATATTTAAAAAATAATCAAGAAATTAAAATTATATCTGCGCTAACAAATTTACATGTAGATAATATGTTAGATGATGTTATTAAAATTTACTTTGCACAAAAAAAGATTTATGAGCAACGACTTAAAGAACAATTACCAGTTGATCAAATTTTAAAATGAACGAGTGATATTCCTAAAAGTAAAGAATTAGATAAAACTATTGAAATTATAAAAGTTGATGATCATATTTTTGAAGTCTTTGGTGAATATTTAAAATATTGAGCACATCGCATTCCCTTAAAAACGCAAGATAATTTAATTCGTTTTAATCAAAAACTTCAAAGCATCAATTTTAACCAGCAATTACTACAAGCTGGAGCAGTAGCGGGGGATAGTATTAAAATATATGATATTACATTAGAATTTGAAGAGTAAATAAATTATGAATAATGATTTGATTAAATATCTACAATGGTTGAAAAATGATTTTATAAAATTTTTAAATGACGCACAAACGAATAATGTTATTTTGGGCATTTCAGGTGGTATTGATAGCACTTTAACATTAGCTATTTTAAATGATTTAAAAAAAGAGTATGATCTTAATATTTATGCTTATTTTTTAGATATTCATAATTCGAGTTTAGATTATGAATGTATTAATGAATTAAAAAATATTTATCCTAACATTGAAGTTATCAATCTTGTTGATATCTATGATAGCTATTGTAAAATGATTAATAAAAAAACAAATGATAAATATGTTCTTTATAATTTAAAGCCTAAAATTCGTACAAATTATTTATATGCAATGGCGAATGCATACAAGGGTGTTGTTGTGAGTAATTTAAACTATGATGAGTACATTTTAGGTTTTTTTACTAAATATGGCGATAGTGCCGCTGATTATTATATGTTAATTGGTTTACTAAAAAAACATATTTATGAATTAGGTGCTTATTATCATTTACCAAATAAAATTTTAAACCGTGCGCCTACTCCAGCAAATGAAGATGATGAACACAAAACTGATGAGAGTTTTTTTGGTTTTACATATAACGATCTTGATCAATTTTTACTTTATCGTAAAATTAATCCTAAAATTGTTAGCATGATTAAAAAACGTTATGAGACTAATGCTCATAAACATTTTGTTTTTGATAAAAAGAAATTATTTTTAAATTATAAATTAGGAAATAAACATGAATAATAAAAAAGGTAAAAAATTTTATGATTTTTTAAAAACAATCGAGGGTTTTAAAATTATTACGATTATTTCATATGTTTTTGTAGCAATTTTATTAGCAATTTTAATCTGATCGGCTGTTCGTTTATCATACTATATCAAAGATTATTATGCGCGAATTGATGAATATAACGAATGAAAACAAAATATTATTAAAATTTTTAAAACCAAAAATTTTCATTTAGATTCTCCTTATAACCAAATTAAAATATTTAAATATGGTTCAATTCTTGGAATAAGTTCGTTTTTAATTATTATTATTAATATTAATATTTTTGCTTATTGATCTTATTCAAACGTTTATATTCCAGAATTAAAAGATAAAAAATTAGTTAAGAATTGGTTTCGTAATTTTCAAAAAAATAATAAAGAAGATCAAAAAGTGATTAAAGATCAACAAGAAGATACTAAAAAAGAAAGCGAGTAAGTGTTCATGGATGTTATTAATCTAATTAAAAATTTAAAACAAATTTTACGAACAGCAAATAATGAACGTCATTTGATTGAATTAAAAAATATTTTTGTTAAACAACATTTATTACCACTATATGATGAATTAAAAAAAAGCGCTAATAAAAAAGAAATGGGTTTATTAATTAATAATTTCAAACAACAAATTGAATTTGTTACTGATCAAATTTTAAAAGAATTAAATGATAAAGATGATCAAGTTGATTTAAAAAAATGAACAAATAAGACTTTGTTTGCTCCTTTTATTAATAATGGGCATCATCATATTCTCAATAGTATTATTGATGATATTGCATCTTTTTTTAAAAAATTAAATTTTGAAATTGTAAGTGGTTCAGAAGTTGTTTCACCTATTTATAATTTTGATCATTTAAATATTGATGAAAATCATCCAGCAAGAGCAAGTGCTGATTCTTTTTTTATTAATTCAATTAAAATGTTACGTACGCATTGCACAACTACAACCGCACAATTTTTAGAAAATAACGTAAACAAGGACATTAGAATTATGTCTTTTGGTAATGTTTATCGAAAAGATGATGATGATGCAACACATAGCCACCAATTCAATCAAGTTGATTTTGTATGAGTTAAAGAAGGTTTAACTGTTGCTAATTTAAAGTGATTAATTGACAGTTTGATTAAATATTTATTTGGTCAAAATTTAAAAACACGTTATCGTTTAAGTTTTTTTCCGTTTACCGAGCCTTCGTTTGAAGTTGATGTTCAATGTTTTAAATGCGATTTAAAAGGGTGCGCAGTTTGCAAAAAAAGTACATGAATTGAAATTATGGGAACTGGAATGCTACATGAAAATGTTCTAAAAGCTGCGAATATTAACGATATTAGAACAGGGATGGCTTTTGGTGTGGGTATTGATCGAATTGCAATGTTAAAATATGAAATTGATGATATTCGTTATTTATATTCAAATAATTTTAAATTTAATGCCCAAATTAAATAAATTCCTAAATTTAATGAAAGAGTAAAAAAAGATGATTTTAAGTTTAAATTTATTACATAAGATTAGTCCAAAACTAAAAAAGATTCCTTTAAATCAATTGTGCACAGCTTTAATGGATTTAGGTTGTGAAGTTGAAACAATTAATAGCATTAAACCATCTACTAATTTAGTTTTTGCTAAAGTATTAGAAAAAACTAGACATCCTAATGCTAATCATTTAAATTTAGTTAAAGTTAAAGCAAACCAAAAAGTTTATGAGATTGTTTGTGGTGCAAATAATTTTAGTGTTCATAATTGAGTTGTTTTAGCAAAATTAAATGCCGAACTAGCAAATGGTTTAAAAATTACGCCACGTGAATTAAGAGGATATGTTTCAAATGGAATGTTATGTGCTTACAGTGAAATTAATCCACAAGCTACTAGTTTTTTAACATCAACAGATTTAGATGGAATTTTAGTTTTAGATGACAATTATGACCATTATAAAACCCCAAATCAAATTTTTAATTTAGATGATGTTATTTTAGATTTATCAATTCCATCAAATCGGAATGATTTAAATGGTTATTTTTGAATAGCTAAGGAATTATGTGCTTATTTTGATTTCGAATATGTTGTTGATGCAACGATTAATCATCGCCCACATAAAGAAATTATTGACGTGCGGATTTTATCTGATGATGTTAATTCGTATGGAATAATCGAGGTTAAAAATATTCAAAATTATATTTTAAAATGGAATACTAAAAGTATATTGATTAATAATCAAATTAAAGTTCTTAATAATTTCGCTGATAATATGAATTTTTTAACATTATTAACTGCTAATCCACTTCATGCATTTGATGCACGAAAAATTAGTGGTCAAATTGTTGTTAAAAATGCTGAAGAAGATGCAATTTTATTGGGTTTAGATCAAAAAGAATATTTAATTAAAAAAGGGGATCTAATTATCGTTGATGATCAAAAAATTTTAGCACTTGCTGGAATTATTGGTTCTAATGATTCTAAAATTGATGCAACAAGCACAACTGCTTATATTGAATGTGCAAATTTTAATCCTATGTTGATTGCAAATACAGCGCGTCGTTTAAAAATCAATACCGCAGCGGCTATGCGTTTTAGCAAACCCTTAACTAATTATGTTACAAAAGTAACATTAAAAAAATTGTTAGCTAATTTTAAATCAGATGCAAAATTAATTTATTATTTTAAACATTCAGTTCATAATGTCATTAAAAATAAAATTAATCAAGTAAGTGATTTTGTGGGTGCAGAAATTGATTTAGACACTGCTCAAACATTTTTAAAACGCTTAGGTTATAAAATTAATAAGTCTAATTTAATTACTCCAAGTCATCGATATGATGTATTAAATGAGTTTGATGTTTATGAAGATATTATGAAAAAAATTAGTATTCAAGAAATCAAACCACAACCAATCAGTTTTGATATTTTAAATTTTGAAAATAATCTAGCTTATGATTTTGAAAAAAAAGTTAGTGATTTTTTAGTTGATCAAGGTTTGTTTGAGTGTAAAACTTATAATTTAAAAAATCAAACACAAGCACATGAGTTTAATTTTTTTAATTTTAAACAAGCTTATGAAATTAATAATCCAATTTCAAATATGCGTTCGCATTTAAAATTAAATAACCTTAACTCCTTACTTGAAGTATTAGAATATAACCAAAACCAAAAAAATGAATTAGAAAATATCTTTGAGATTTCAAAGATTAATCCAGTTGATTCAAGTCAACAAACAGTTTTATCAATCATTTTATGTAAACCATTAATTAATTCAAAAATTAATGATTCATTAATAGTTAATAATTTTGTGACAACAAAAGCTTTACTACATGCTTTATTAACAAAATTAAATATTGATTATGCATATGATAAAAATCATGTTGTTAATGAATTATATGATAATAACCAATTAGCTTTAATTAATGCAAACAAACAAGTATTTGGTTTTATTGGTCAACTAAAAAATCAAGTCAAAAAAACATATGGTTTAAGTAATGATATTTTTATTATTAATTTAAATTTAACTAGTTATTTAAATCAAAAACAAGTAATCACTAAAGTTATTAAGCCAAGTATGTATCATGATGTCATACGTGATATTAGTGTTAAATTAGCTTCTGACGTTGACTTAAATAATATAATTACTAATATTAAAAAAATTAAAAATATTAGAAAAGTTGAAATCAGCGATCTTTATATAAAAGATGATGGAATTATTTATACTTTTAAATATTATATTAATGATCATTTATCTAATTTAAGTAGTGAACAAATTATAATCATTGAACAAGAAGTAAATAATTATTTGAAACAATTTTAATTTTGTAAATTCTTTAATTAATAAAAAAACACCTGCAGTGGTGTTTTTTTATTAATTAGTACATTTCAATTACTATAAGAAATGGTTTTTAAATAAAATAGTTACTAAAATTAATTAAGAATTAATATTAATTAACAAGTTGATTAAATTCGTTAACAGTTTGCTCTAGTTTTTCAATATTTTTCACTAAATAAGAACCGCTTACAAAAAAATCAATTGGAATATTAATTTTTTTAATAACTTCTAAAGTAATACCACCATCAATTTCAATCTTAAATTTGAAATTATTTTTAATTTTATATTCATATAATTTTTTTAGTTTTAATAAACTTTCTTCTAAAAAAATTTGTCCACCCTTACCTGGAATAACCGACATAAAAGTAATATAATCAACTTCTTTTAAATAAGAAGCATAATCGTTTAGTGATAAACAAGGATGAATTGCAATCCCTGCTAAAACATGATGTTTTTTAATTTCATTAATGATTAATAAATTAGTTTGATAATCATTCATTTCAAAATGAAAAGATATGCGAGTTGGTTTATATATAAAATATTGTTTAATTTTTTCTAAGGGGTTAATAACCATTAGATGAATGTGTACTAAAAAATCATTTTTTAGTAGCCATTCTAAATGTTCAGTTTCAAAAGCAGTGTTATTAACATATGTTAGATCCATAACATCATAATGTATTGTTTGAAGATGATGCTTTTTAAAGATCTTTAATTGTTCATAAGGATATTTTTTATCAAATGCTAAAAGGGATGGAACTAGTTGCTTATTCATTATAGATTATTTATGATTCAACCTTTTAAATTACTATTATTAGCTAAACGTTTATCGTAAACTTTAATTTTATGTTCGTTTTCAATTTGACTAAAAGCTTCAATTTTTAAAGTTTGATCATTTAATGCTAAATCAAATAATAAGTTATAAAATTGGCTTTTTGACAATCCTAAATATCCTGTTGCGCTTTGATTTAACTGATTGTTAATAACTAAATTTTGCACATCATTTGCATTAAATTGTGAAACCAATAAAATATATTTTAAATTAGCATTATCTTCTTTATTAACTAATGGTGTAGCATTTTTGTCTAATTTGTTATAAACTAAATCTAAATCTAATCTTTGAAAGGCAGTTTGTGGGATTTTATTGATTCCTTTAATCATAATTGTTTTAATTTCATTAGTTGCTAAATCAATATTTTTTATTTTACTATCTTTTTGATTACCAGGAATAATTTGTTTTTGGTTATCTAAAATAGTTTTAAATTCAGCATTAGCTTCTGCATTAAAATTTAGATCATAAAGTAATTGTGCAAGTGTTTGGTAGTCATGATAAGACACAAAACCATCTAATGTTTTAATTAATAATTCTCTACTACCATAACCATATAGTGCACCTTTAAATTGTTTACTTGGGTTATTAGCGTAAACTAATTTATCAAACAAGTCGATTTTTTCAAAAGCTTTACCTAATTTATCTTTTTGATTATTTTGATCAATTAAGCCAATAAAATTATTATAAACTGAACCATCTTTATTAACGGTTGAGTTTCAATAATTTTTATCGTTAGTGTATGTTAATGCATCTATAAAAGTTTTTTTGAATTTAACTTCTTGACTATTTTGGTCAATTTCATAAACATCAGCTTTTTTTAATTGGTATAGGTCAAGTCCATTATATAAATTAATTTGGTATTTCTTGAAACTAAAATCTTCTTTAGAATCTTTTCCAAAATTAGGAATTTTAGTTACATCATTTAAAGCTTTTCAACCAATAAATAATGGTTGATGATTAGCAGTTTTTTTTAACAAGTAATTAATTAAATTTTGATATGTTGGTTTACCATCTTTAAAATCTAACAAGTAATTAATAATAGCGAGTGTTTCAAATAATGCTTGGTATTTTTGATAATTGATTGATAACTGATTAAAAGGGCCATTTTTAGTATAATTATCGTTTCATAATTTCAAAGCTTGTTCATAAACTTTTTTTAAGTTATCAAAATTACTAAAATTATTAGTTTGTGTTGTTTCCAAAACACTTGTATTCTTAATAATTTGGTTAAATGCTGCTAATAATTCTTGGTTTTCAAGAAAATTATTTTTAAAATTAAAATCATTAAATTCAAAGAATTTTGCTACACTTTTTTTAATTTGTTCTTGTTTAATAATATTTGCAAAAACACTGTTTATTCCTTTACTAAATACTTTATTTATAACATAATCGTCAAAAACAATTGATTGGGAGTTTTTATTATTAGCAACAAGGTGTTTTTTTATATCAAAGTTTTCAACATAATCATTAATCATTGTAGATAATTTTTGATTTTCATCTATAAGATTTTTATTACCCACTAGTTTTATTTTTAAAGTTTGAAATAAACTAATTTGTTGATTTTGGTCTTTAATTAAGTAAATTGGTGTATTAATTCCATTATCATATAAACTATCAACACTAGGAATAATAAATGTTTTTTTATTAAATTTAATTAATTTATCATTTAACTTATCAATTAAATTATCATAGTAATTTAATAAATGAATTTCATTGTTTTCTTTAATAATATTAATTGTTCTTTGATCAAATGCAAAAACTTTTGAATCATTTTTTAAGTTTGATTTAAACAAAAAATCACTATCGTTTTGATGTGTAGCAATATATAGTGCTAATAAGCGCTCTTTATTGTTATTAAAGTAATCTTTAATTTTAGCTTTAATATCATAGCCTTGTTTTTTAACACTATTTGATTTGCGTGAATTTAAGTATAGTGCACCATTGTATAAAAAATCATTTTTTAACTCTGTTAATAGAGCGTTCAAACCCATTTTAGCTGCTTGTTGAAGTCTTTTAAAATTGTTAATACCTCATAAATCAACACTGTTTTCATTACGTAATAGAGTATATCCATTTTTTAAATCATTAATTTGACGGATAGCTTTAACATCCTTAAAATCATTTAAAAAAGCTGTTTTATCTTTTTTAACTAAAGGATAAAATAAATCAACATTATTTGTGTTTGCATTACTTTGATTCTTACTAATAAAATTGTTTAAAATATTATTTTCATCTAAATTATCTAATTGATAAATACCAAAATTTTCATTTCCAAATAATGATAAAAATTTAGCTAATAAACCAACACTTAATGTTAAATTTGTGTTGTCTTGAGTAATAAAATCATTTAAGCTTAAATATTTATTAATATCATTATTGTTATTGAATTTTTTGTCAATATTATTCGTATCATCTTTTGAATCTAAGTATTTATTTTCTTTTAATAATTTAGCTACTTCTAAATAATTAGTTGTAGTGTTAGTTGATTTATTATCAGTTGGTTTAGGATCAAATCAAGGAAAATTAGGTGATGTGCCAGAAGTTGGTATTTTTGTAAAAAAATCTTTATTATAAATGTTTTGATTTTTTAAATCATCATATTTAAATTCAATGTTGGCAAATGATAAAGGCAGGTTGTATTTAATATAATCATCAATTAAGAAATTAAATAAATTAGCGTATCCTTTTTGTAAAAAATTATTTGTCTTAGCAACAATTTTGTTTCTATCTCCAATTTTAGTTGATTTAAAAGTACTTTCATTCTCTAACAACTTAGTAGCATCATTTGAATAGGCATCAATTAGTTGATTTTGATCATTTAATACACTAATTCAATTATCATTATCATCAAAAATTGAAGAAGTAATTGATTTGTATGCTTGATCAATTAACTTAGCTTCTTTTCATGATTTTTCACTACCACCTTCAGTATCATATAGTTCGCTTTGTAAAACAGGTTGTCAATCATTTGGGTATGATTTTTTTACATTTTCTTTAATTCTCTTTAATTGTTCATCTAATTGTGTATTATATTCACTAACATCATGAGCTAATTTTGGATCATTATTGTTTTTTGCTCAATCATAAACCACTTTTTTAACATTTGATAATAAAAATTCATTTTGTCCTTCTTTTGAGAATAAAACATCTTTTAAAGCTTGTTTTGTAGTTAAATTACTTGTTAAATTTAGACTATCTAATTTATTTATAGTTATTTTATACTGGTTAATTTTTGTTTTCGTTTGTGAACAATTTGTAGCTAAACTTGCAATTGAAATAACCCCTAAAACACTTAATGTGCTTGTTAAAAGAATTTTTTTAAATTTCATATCAAACTCCATATTATTTTTGCTATGAGAATATATTATAATTTTATCGTTTTAAACAATATAATTTTAATATTATACTATAGAAGTATGTTAGATTTTTTCTAAAGTTGAGCTGACAAAAGCAAACTTTGTTATTGTTTTTAACAAATTTAATTATCAAGCCATCGCAAGAAATTTAGTCTTATTTTTGAAAAATAAGCAAGGATACGCTATGAATGATTACAAACAATTTTTTCCTTGATTTAAAAATAACAAAGATGTTATTTATTTAGATTCAAGTGCAACTTCATTAAAACCTCAAGTTGTAATCGATGCAATTGTTGATTATTATACAAAATATTCTACTAACCCCCATAATACTGATTCAAATTTTACTTTTCACACTCATGAAATTATGAATGAAACAAGAGCAAATGTTGCCAAATTCATCAATGCTAATTTTGATGAAATCATTTTTACAAGTGGTGCTACAGAATCTCTAAATTTGATTGCAAACGGTTTAAGACCTCATTTAAAAAAGGGTGATGAAATTATTTTAACTTATATTGAACATGCTTCTAATTTATTACCATGATACAAACTTCGAGATGATTTAGGTGTCAAGATTATTTTTGCTAATCAAAAAAATCAATTTCCACAACTTAGTGATTTTCTAAAAGTAATTTCACCTAAAACAAAAGTGGTGTCGTTTGCTTCTGGTGGTAATTTAATTGGTAATGTTTTAGATGAAAATCTTATTATTAAAAATATTAAAAAAATTAAACCAGATGTATTAGTGTGCGTTGATGCAACTCAAAGTATTCAACACCGTATGTTTGATGTGAAAAAATGCAAAAGTGATTTTATGGTCTTTTCAGCACATAAATTATTAGGACCTACAGGAATTGGTGTTGCTTATATTAAAAATGACTTGATTAAAAAAATGCAACCATTAAAATATGGTGGTGGAATGAATTTTAGCATTGATTTAAATAGTTATCAGCTCTATGATAATTACATGAAATTTGAAGGTGGAACTCCGCATGTTGCTGGTTTTTATGGTTTTAATGCTGCTTTAAAATTTTTAATGGATATAGGTTATAATAAAATTCATGAACATGAATTAAAGATTACTAAATATGCACGTGACCAATTAGCTTTAATTCCTGAAATTAAAACATATGTGCAAGATATTACTTCAAGTACTATAGCTTTTAATTATAATGGTGTTTTTTGCCAAGATTTTGCTAATTATTTAGGATCAAAAAATATTATTGTGCGAAGTGGATTATCATGTGCAAAAATTATTAATAACGTGATTCAAACCGAATGTGCTATTCGTGCATCTTTTTACATATATAATGATTTTAATGATGTTGATAGATTAATTAAGGCAATTAAAGAATATCAAAAAGGAGATGAATTAAATGGCATCTTATAATATCAATGACAATCTTTTATTACGTAAGATTATCATGCAACACTATGAACACCCTAAAAATAAAGTTTGTTTTGTACAAGATGAAATAAATTATTTGTCATATCATAATACAACAGAAGGATGTGGAGATGACATTACAGTTTATGTTAAATTAAATCATCAAAAAATAATTGATGTCGTTTTTTTAGGTACAGGCTGTGCAATTTCCATATCATCCACTGATATTATTTGTGAACTTGTAAAAAATCTAGATCTTAATAATGCGTTATTATTAATTAATAATTATTTAAATATGATTGAAGGGCTAGATTACAATAAAGATATTATGCAAGAATTAATTGCTTTTTATAATGTAAAAAAACAAATGAATCGTATTCGTTGTGCCCGTATTGGCGCAAGTGCTTTAAAAATGTGTTTAAAGCAATATACAGATAAACACAAAACAATTGTTTAATAAAAGTTTAGGTTGGATTGATAAAATGAGTAAAAAATTAGAAAAGATTATAACAAGAAAAGAAAATTTTGCTGATTGATATACAAGTATTGTAAATAATGCAAAATTAATTCAGTACACTGATATTAAAGGAATGATGGTTTTTCAACCAAATGCTTGAGCAATTTGAGAAGCAATTAAAAACCAAATTGATGTCGAATTTAAAAAACACGGAGTACGCAATCTTGCCATGCCAACTTTAATTCCTTTAAGTGAATTTCAAAAAGAAAAAGACCATATTGAAGGTTTTGCACCTGAATTATTTATGGTTAATCAAATTGGTGATAAAAAATTAGATAATTCTTATGCAATACGTCCTACGAGTGAAATTTTATTTTGTAATTATTTTAAAAATATTGTTAATAGTTATAATGATTTACCGATTAAGAATAATCAATGGTGTTCAGTAATGCGTGCTGAAAAAACAACACGTCCATTTCTTCGAAATGCTGAGTTTCATTGACAAGAATTACATGCTATTTTTGCAAGTGAAAATGAGGCCGATTCTTTTGCTAAAGTAATTTTAGATGTTTATACAGATTTTGTTCAAAATTATTTATGCATACCTGTTATTAAAGGATTAAAAACACCATGAGAACGTTTTGCTGGTGCTCAAAAAACATATACAATTGAAGCTATGATGCAAGATGGCCAAGCATTACAATCTGCAACAAGTCATTATTTAGGGCAGTTTTTTGCAAAAGCTTATGATATTAAGTTTCAAGGTCAAGATAATCAAATGCATTATGTACATCAAATGTCTGCTGGATTATCAACACGAATTATTGGAGCATTAATTATGGTTCATGCTGATGATCAGGGTTTGATTTTGCCCCCTGATATTGCTTTTAATCAAATTGCTATTTTAACGATTTTTTCTAATAAAAATCCCCAATTATTAAAAATTAGTCAACAGATTTGTGAACAATTAAATGGTTATCGTTTGTTTGAAGATCACAGTGATAAAGGAATTGGTTATAAATTAGCACAACAAGAAATTGAGGGAACGCCTATTTGTATATTAGTTGGTGCTAAAGAATTAGTTAATCAACAAGTTGTTATTGTACGTCGTGATACTCATGAAAAAATTAATGTTGATTTAACTGATTTGAAACTAATTATCCCAAAATTATTAGCAGATATTAAAAGAAATATTTATGAAAAAGCAAAAAAAGATTTAGAGGATTCGATTGTTTTTGTGAATAATATCGAGGAATTAAAACAAGTTATTGCACAAAATAAAATGGCTAAGGCATTCTTTGATGGTACAAAAGAAGATGATGAACAAATTAAATTATTAACTAATGCTTCAACACGTTGTATTTTTGATGAAACTCAAAACGGGCAGTGTTTTTATACAAATAAAAAAACAAACAAATTGACTTTATTTGCACGTGCTTATTAGATTTAATAAATGAAATAAAAAAGTTTATTTTCATATATAATTTAAGTTAGTTAAATGAAGTTAAAATTATAAAAATAATTAATTTTGGAGTTGAATTATGGGATTATTTAGTAAGAAAAATAAATTAAATCAAAAGTTAACTGAAGATAAAAATATAAATATTACTGATGTTTTAGATAATAACAAAGATATTTATCAATTATATGATGAAACTATTGTTACTAAAAAAGTTAAAGTAAGATGCAAAAATTGTGGCGTTTTTATGTGAGTTGATTACACAAAAACGATTAAAAGTTTACGTAATTTAGCTTTAACAAACATCCAACAAGAACAAATACCTGTAATGGATGAACCACAAATTTTACAAGATTGCAATGATCTATCATTAGCTCCAGTTAATAACCAAGAAGATGATATTGTTAACGAACATGAATCTACACCTATTGAACCATTACAAGAAGAAGTTGAACCTGAGCCTATAAAGAACGAAAATCAAAGCAATGAAGAATTTATACAAGAGAATATTGTAGCTCCAGTTGATGAAGTTGATAATTTAGTAAAAGAACATACACCTTTTACTCCTGTTTCTTTTGAATTTGAAGAACCTGTTTTTGAAAATATTTATTCAAACCAAAAAGTGTATGCTGAAAATTTATTTTTAGAATCTTTATTTTCTGAAAAAGAAAGTTTAATTAATGATGATCAAAATGATTTTAGTTGGTTATCAAAGTATGTGAGTGTTTGAAATAAACGTGATGAAGCTGAACTAGCACAACTAGTTTATGATGAATGTTTATTAGCACCTCAAAGTTCGGTATTTCCTAATAATGAATACTTTATAGAAGAAGAATATGAACATATTGATCCAGATAATCACCATAATAAACATAAAGCGCAACGTCATCATCGTGGCCATGATTTAATTGTTAAAAAAAATGAAAAAGAACAAATAGAAAAAGCAATTGATGATGTTTTACCCATTAATAATGGTTTAGATAAAAAACAACAATTATTATTAAAAACAGTATTAACTGAAGAAGAATTTCAAAGTGATTCAATCTCAAAAAAAACATGATGAGAAACTGAATCAGCAAATAAATCATCAAAAAAGTAAAATTTTTAGAAGGGGGCGCCCTTCTTTTTTATTAGGAATAAAATTATATAATTAAATTATCAGAATAAGGATTTTTTAAAATGGATAATAATATTAATGAAAAAACTAGTTTTTGAACGAAGAAGAATATTTGTTTTTTAATAATTGGTTTAATAGTTTGTTTATTAATGATCATTATTTCAATTCTTTTTATTTTAGATATTGATTTTCAAAATTTATTTATAAAAATAAATCATAGTTTAAAAACTATTAAATTAGCTATTTTATGATTGTTAATTCTTATTAGCTTTATTTTCTTTAGGGCTTTTATGTTTATAGTTATTGCAGTCATTGATACTTATAAAAACCAAATCAAAATTGCTTGATGGAAATGAATCCTTTATAGTTTTAGTTTAATTTTTTTAAATGCTGTAACTCCTTTTTCTGTTGGAAGTGAACCTTTTTCAATTTATTTTTTAAATAAAAATGGTTATCATAATCTAAAAAAAGTTTCTGCACTATTACTTGTTTCATCAACTTTTTATGAATTGGGCCAAGTTATAGTAACTATACCATCTTTTATTTATATTAATTATGAATTAATAACTTATGCAGTTAATAATAAAACAAGTTTACCCTTTTATTATTATTTGGCTGTTGTTGGTATAATTGTTGATTTATGTATGACAGCTATTTATTTTGTTTTAGGCTTCTCTAAAAAATTGCATTTTCGTTTAGTTTTAATTTATAATTGAGTCAAAAAACTTTTTAAGCTTAAATATTTAACAAAAATTGAATTAATTCAAAAAAATAATCAAAATATAAGTTTTAAAGCTTTTTATTTGTATTATTTTAAAACACTGCGGGTTTCATTATTGGCTTTTGTTATTGGAGTAGTCTATAACATTGGTATTTATGCACTAATGTATATTTCATATCGTTTAGTAGAACCAAAACCACATAACATCTTTTTTGATTTATTTAATTATACAAATATTGCTGTCACAGCAACTAATTTTGTCCCTTTGCCTGGATCTGAGGGTTCAATTCAATTTATGTTACGTGTTTTTTTAACAAATGATCAAAATCAAATTCGTTACGCAATGAATTGATTATCAACGCAAGATATAAATAATATTATTTTTATTTGAAGAATGTTTAGTATTTATTTAGGTAGTTTAATTGGGATGTTTGGGTTTGGTTATTTTTTAATTTTTGATTTATATAAATTTATTAAAAAAGCTAAAGTTGATAAAAAAAGATAAGAATAATTAGTTTTAACTAATTCTTTATTGAGGTGGTTAAAATGAACTATTTAATTTTTAAAGTTATTTATGCAAATGCAAATATTGTTGTTGGTGAACATAATTTTATTGGTTATCAAATACGTATCCCAAAAGATTATGAATTAGAAATTAATAAATTTTGCAAATTATATTTGTATGAATATATTAGCATAATGCCTAATAAAAATTTAATTATTAAAGATTTATATGGTTTTCGTACTTATAATGAACGGTTATTATTTATAGACTTGATTAGTACTAATTCAATTGGCCCAAAAACAGCTATTAATATTCTAAAATATGATATTGATACAATTATTGATGCAATTGCTACTAAGAATATAGATTATTTAATAACAATTAAAGGAATTAACCAACGAAATGCTAATTTGATTTGTGATCAATTAAATTATAAATACATAAATAAAGTAAATGATAAAAATAACTGAGCTAAAGAATTAAGTAATAGGTCTTGAAAATTTAGGATATACTAAAAAAGATATTGAGTATGCTATTACTAAAGTTAAAATTAATTCTCAACAAGATATTGATATTAGTGAAATTATAAGCTCTGCAATCAAAGAAATTTCATTGCGCCATGAAAACTAATTATGAGTTTCGTCCACAAATCTAAAAGATTTTATTGGTAAAGAACAATTAAAAAACAATTTAAAAGTTTATTTAACTGCTAGTAAACGATTAGAAAATAGTTTTGATCATACATTATTACATGGATTATCAGGAACAGGGAAAACAACATTAGCACTTATAATTGCTAATGAAATGAATGTTAATTGTCACATAACACAAGGGAACTTGTTAAATAAACCTATTGATATTATTAATTTATTATCTTTAATTAAAGAAAATGACGTTGTTTTTATTGATGAAATTCACGCTTGTGGTTTAGGTGCATTTGAAACACTTTATTCTGTATTAGAAGATTTTTGTATTGATATTAATATAGGTAAAGATTTTAATTCAAAAATGACGAGGTTAAAGATTCCTCATTTTACACTTATTGGTGCAACAACAATTTTTGGAAAAATCCCAAAACCTTTAGAAGAACGCTTCGGACACATCTTTCATTTAAATGAATATGAACCATCAGAAATTAGTGCAATTATTTTAAAGAATAATCAGATGCATTTTCAGATTGATTTAAACGAAGAAGAAATTGATTTGATTGCTAATAATGCTAAAGGAATCCCACGATTAGCAAATCGATTATTAAAACGAGTTGTAGATTTTAAAATTAATGGGTTTAATGATATTAAAAATATTTTTAAAAAAATTCAAATTTATGAGTTTGGATTAGATGAACAAGATATTAATTATCTAAATGTTTTATATCGACAAGATAATGAAATTGGTTTAAAATCAATTGCACAGATTTTACGTTTAGATCAATATACAATTGAAACAAAAATAGAACCTTATTTAATTCAACATCATTTTATAAATAAGAATTTAAGAGGTCGAAAAATTACAATTGAAGGAATAGATTTTTAAAAAACAATCAATTAATCAAATAAAAATAATTGTTTTTTAACATTTTCTTTTTTTACTAATTTATTTAGTGAGATACCAATTAAACGTATTGTTTCATCTTTATACATTATTTTAAATAAGTTTTTAATTGCTAGAAAAATGGTTTGATAATCATCAGTGTAATAAGTTAATTGTTTTTGCTTAATTTTTGTTTTAAAACTAGGATATTTAATATAAATACTGATGTTATTAGTCATTAAATTACATTTTTTTAATCGATTAGTAACAGTTAAAATTAAATCGTTCATTTCATTAGAAATTTCATAAAAATCATTTAAATCTTCGTTAAATGTTTTCGAATGTGAAATTGTTTTATAAATATCTTCATCAATTATTTTAATTTCACTTAAACCTTTTAAATCATTGACTAAATTTTGATATATTGAACCTAAAACTAATGAAATTTTTTGTTTATTTTCAGTTGCAATTAATTCTTTAATTTTAAAAATGTTAATCGCATATAATTCTTGTGATTTTATTTCGCCAATATAGGGAATTTTATTGATATTTAATTCATAAATTAAATTAATAATTTTATTTTCATCAATAATTGATATACCAGAAGGTTTATTTAATTCCGTTGCCATTTTTGCAATTGTTTTATTATATGAAATTCCAATCGAAATACTAAGGTTTAGTTTGTTTTTGATTTCTTTTTGTATTTTTGTTGCTAACAAGCGGATATTATTATGATATTTTTTAACTAAATCTGTTGCATCAATAAAGCATTCATCAATTGAAGTTATTTCAATCTTATTTGCATATTTATTAATGATTTGAAAAAAATGTTTTGAATAAGATTGATAAATATTGTGTTCAGAAGGTAAAATAATTAAATTTGGACATAATTCTTTAGCTAAAAAGATAGGCATTGCAGCTTTAACGCCATAAGAACGTGCTAAATAGTTAGGTGAAGAAATAATACCTGTGCGTGAATTTAATGAACCAACAGCAATTGGAAAATTTTTATATTCAGGATGTAATAGTTCACTAACAGTAGCATAAAAAGCATCGATATCAAGATGCATGATGATTTTTTGATGATTATTTTTCATGCTTTTGTAAAAATATCATATATTCTTGATTTTGTTTTTTTTCATCAAAAATATCAGTAGGAGTTAAATGTTTACATTCAAAGTCATAATTTTTTGCTAAATTGATTATTTTGTTTATTGCTAATTGTTGTAATTTTTGATTTTGAATTAGACCTTTATATTTACTAGCTACGTCCTTTCCTAATTCAAATTGGGGTTTTATCAACAATAAAAGCAAAGTTTTAGGGGATGAAATTTTTTTAATTATTGGAAAAATTTGCTCTAAAGAAATGAAAGATAAATCACCAACAATTAAATCTAAATCTTCTAAAAAATAAGATTTATCTAAATTTTTAACATTAGTTTTTTCATAATTAATAATTTTATAACTTTTCTTTAAACTAGGGTGTAATAAGTCAACACCAACATCGATTGCGTAAATTTTTTTAGCACCCAATTGTAAACAACAATCAGTAAAACCTCCTGTTGAGCTCCCTAAATCAGCTATAACAAAATTTTTAATTATTAAATTAATTTCTTTAATAATTTTTAAAAGCTTATATCCACCTTTAGAAACGAAATACATTTCATCATTTTTAATTTCTACAGAATCAGTCTGTTTAACTAAAAATGAGGGTTTTAATACTTGAAAATTATTAACAAACACACGTCCGCGTTTTATCAAATCAATTGCTTTGGATCGTGTTTCACATTGATTAGTGTTTTTCAGATAATGATCTAACCTTTGATTCATTTATAAGCTTACCTTTTTTATAAATTATTAATTAAAATTAAAATAAAAACCACTTCAATGCGGTTTTTATTTTAATTTTAATTTATTTTTTTGTTTTATCAACTTCTTTTTCTAATTCATTTTCAATTTCTACTTCATCACCATCATTATAAATTGAATTAGTTTTTTCGTTTTCTTTGATTTCATTAATTTTATAGTCTTTTGGTAAACTAAAATCTGTTACAGAAACTTGTGGATATGTCGATTCATTTTTCACAATAATTGATTCAACACCAAAAACATCTAATAAAATTTCATGATCGATTGTTTCTTTTAATTTTCCATAACGAACCATTTCACCATTTTTCATAATCATGACTTTATGTGCATATTTTAAACCATTATTAATATCATGAATAACTAAAACAATTGTTTTTTTCATTTCGTGGTGTAATTTATGTAAAAGTTCAATAATTTCTAATTGACTTCTTATATCTAAGTGGTTAGTAGGTTCATCTAAAACAATTGTTTCAGTATCTTGAGCAAGTGCTAAAGCAATTAATGCTTTTTGTTTTTGACCACCTGATAAATCTTCAAGATTTTTGTGGCGGAATTCATAAATACCAACATTTTTCAAAGCTTCATCAATAATTTGTTTTTCACGTTCTGTATTTGTATTAATTCCCAAAGCATTTGAACTTGGAAAACGACCCATTTTAACAAAATCATAAATTGATGTAGCTTCTGGGAATACAGCAAGTTGTGGGACATATGCTAAATTTAAAGCTAATTCCTTTGACTTATAGGCATGAATTTTTTTATAATTTTGAATATGTCAATCAATTGTTTCAATAACTTCTTTACGATCTTTTGTAAATATATTAACAATAGAAGATCATGCTTTTTCTAATCAAAGAAATGGTAATAATGGTCTTGATATCAATTTATCTCTATACATTATATAACCACTTGAAGGATTATTGATACGACACAATCCTTTGATTAATGTGGATTTACCAGAACCATTAGGCCCTAATACAACAACAAAATCGTTGCGATGAATTGTTGCCGTTAGGTTTTTAATAATCATTTTTTTTCCGTAACCTAATGAAACATTATTAAGTTTAAAAATTGGCTCATTAGAATCAATTTCATCGTTTTTAAATTCTTCACTATCTTCATAGCTTAAAATTTCATTCATATCTACAACACTAACGTGTTTGTGTTCTTCTTTTTGTAAATCATTCTTTTTTTTAAAAAAACTCATATTTACTCCCAAAACTAAAAAATTAAATTATAAGTTAGAATTTCATTTTATCTACAAGAAGTTTATCTTGTATTCCCATAAAAAAATAAATGCAAATTATTTTTAATTTCATCATTAAAGCATTTATTATTTTTGTTTTTATTAATTCTCCAATAAGAATAACACATTTTTTTATTCTATATATTTTACATTTACCTAAAAAAAACACTTTTTTTTAATTTTAGTGTATTTTTTTGTTTTAAAAGCGTTAAAATAAAATTGCATTATTACTTAATATACAAGATATATTAGAGGTAAATAAATGAATCTATCATTAAGAGAAGTCCAAAAATTATTGATAACAGTTGCTGCTGACGTTGCAAGAAGACGTTTAGCTAGAGGTTTAAAATTAAACTATTCAGAAGCTGTTGCTTTAATTACTGATCATGTAATGGAAGGGGCAAGAGATGGTAAGCTAGTTGCTGACCTAATGCAATCTGCTCGTGAAGTACTACGTGTTGATCAAGTTATGGAAGGTGTAGATACAATGGTTAGTATAATTCAAGTTGAAGTTACTTTCCCTGATGGTACTAAACTAGTTTCTGTACACGATCCAATTTACAAATAACATTTACAATTCGTAAAATTTTTTTATAAAAGGAGATAATGATTATATGTCAGGATCATCAAGTCAATTTAGTCCAGGTAAATTAGTACCAGGGGCAATTAATTTCGCTAGTGGTGAAATTGTGATGAATGAAGGTAGAGAGGCAAAAGTAATTAGTATTAAAAATACTGGGGACCGTCCTATACAAGTTGGATCACATTTTCACTTGTTTGAAGTGAATAGTGCATTAGTATTTTTTGATGAAAAAGGAAATGAAGATAAAGAACGCAAAGTTGCTTATGGACGACGTTTCGATATTCCATCAGGTACTGCTATTCGTTTTGAACCAGGAGATAAAAAAGAAGTTTCAATTATTGATTTAGCCGGAACACGCGAAGTTTGAGGTGTAAATGGCTTAGTTAATGGAAAACTTAAAAAATAAAACTCTATTTTACAAGTTTCTACTATAGATAAAAAGGGGAACATTATGTTTAAAATTTCAAGAAAAAATTATTCAGATTTATACGGTATTACAACTGGTGATAGCGTTAGATTAGGAGATACAAATCTTTGAGTTAAAGTTGAAAAAGACTTAACTACTTATGGTGAAGAATCTGTTTTTGGTGGTGGAAAAACCCTACGTGAAGGTATGGGAATGAATTCTACTATGAAGTTAGATGATAAATTAGGTAATGCTGAAGTAATGGATTTAGTTATTACAAATGCACTAATTGTTGATTATACAGGTATTTATAAAGCAGATATTGGTATTAAAAATGGAAAAATTGCTGCGATTGGTAAATCTGGAAATCCACATTTAACAGATAATGTTGATATGATTGTGGGTATCTCAACTGAAATTTCAGCTGGTGAGGGTAAAATTTATACAGCTGGTGGTTTAGATACTCACGTTCACTGACTAGAACCAGAAATAGTTCCTGTTGCATTAGATGGTGGTATTACAACTGTTATTGCTGGTGGTACAGGTATGAATGATGGTACAAAAGCCACAACTGTTTCACCTGGTAAATTTTGAGTTAAATCAGCTTTACAAGCAGCTGATGGATTATCAATTAATGCCGGTTTTTTAGCTAAAGGTCAAGGTATGGAAGATCCAATTTTTGAGCAAATTGCTGCTGGAGCTTGTGGACTTAAAATCCATGAAGATTGAGGGGCAACAGGAAATGCGATTGATTTAGCATTAACAGTTGCTGATAAAACTGATGTAGCTGTTGCTATTCATACAGATACATTAAATGAAGCTGGATTTGTAGAACATACAATTGCAGCTATGAAAGGGCGAACAATTCATGCTTATCATACAGAAGGTGCTGGTGGAGGGCATGCTCCAGATATTCTAGAAACTGTTAAATATGCCCATATTTTACCAGCTTCTACAAACCCAACAATTCCTTATACAGTAAATACAATTGCTGAACATTTAGATATGTTAATGGTATGTCACCACTTAAATCCTAAGGTTCCAGAAGATGTTGCTTTTGCTGATTCACGTATTCGTAGCCAAACAATTGCAGCTGAAGACTTATTGCACGATATGGGTGCAATTTCAATTATGTCATCAGATACATTAGCTATGGGACGTATTGGCGAAGTTGCAACTCGTACATGACAAATGGCTCACAAAATGAAAGCACAATTTGGATCATTAAAAGGTGATAGTGAATTCAGTGATAACAATCGTGTAAAACGTTATATTTCTAAATATACAATTAACCCAGCTATTGCACATGGTGTTGATTCTTATATTGGTTCACTAGAAGTTGGTAAATTAGCTGATATTGTTGCTTGAGAACCTAAATTCTTTGGTGCAAAACCTTATTATGTTGTAAAAATGGGTGTAATCGCTCGTTGTGTAGCAGGTGATCCAAATGCTTCAATTCCAACATGCGAACCTGTAATTATGCGTGACCAATTTGGAACTTATGGACGTTTGTTAACTAATACATCAGTAAGTTTTGTTTCAAAAATTGGGTTAGAAAATGGCATTAAAGAGGAATATAAATTAGAAAAAGAATTATTACCAGTTAAAAATTGCCGTTCAGTAAATAAAAAGAGTATGAAATGAAACTCTGCGACTCCAAATTTAGAAGTTGATCCGCAAACTTTTGATGCTGCTGTTGATTTTAATGATTTAGAAAATTGACTAGAACAATCAGCTTCTGAATTAGCTAAAAAATTAAAAAAGACTTCAAGTGGCAAATATATACTTGATGCTGAACCTTTAACAGAAGCGCCATTAGCACAAAGATATTTCTTATTTTAATTGTCAAACTAAAATATTATATTAATTTATTTTCGTTACATTTTAAAAAAGCGAGGTAATAATCTTGACTGTATTTAAAGAAATTTTAGATAATATTGCTAACATTAAAAATGTTGAAAGTTATCAAATTGAGAACATTCATTTAACAAGCGACGACGTTTTAAAACGTGTAATTATCATTTCATCAGATCAAAATGTTGAATATGGAATTCGTTTAGAAGAGGACAAAAAATTAATGGATGGTGACATCTTATACAAGGATGATTATAAACTAGTTGTTATTAGATTAGAGTTATCAGATGTATTAATTATTACAGCACACACAATTGGTGAAATGGCAAAAATTGCCCATAATTTAGGTAATCGTCATATGCCGGCTCAATTTACTGAAACACAAATGATCGTTCCCTACGACTATTTAGTAGAAGAATACCTTCAAGATAATAAAGCCCTATATGAAAGAAAAAAAATTAAACTTAAAGAAGCATTTAAACACTGTAGTGACGCTAAATAGTGACTATTTAAATTTATTAGACCTAATGCAGATCACTAACGCAAACTTTCCAATTGGAACATTTAGTCATTCTTTTGGAGTTGAAACTTATATTAGAAAAGATATTGTTTTTGATGGAGAATCATTAATTAAAGCGTTACTTCTGTATATGAATGAACAATTGTTACATGGTGATTTATTGGCAATTTATCAAATTTTTAAACTATTGCCAAAACAAAAAATAAATGCAATTTGAGAAATTGATCAAATGATAAACTTTCAAGGTTTAGCAAGAGAGACTCGTGAAGGTCAACGTCGAATTGGACAACAAATGGTAAAGATATATAATGAACTTTTTAATTGTGAACTTTTAGTTGAATATGCTGAAAGAATAAAAAATAAAAAATCTTATGGTAATCCTGCTGTTGCGTTTGCTTTATTAGCTATGCATTTAAAAATAGATTTAAAAACTGCTTTATATACGCATCTTTACTCTACAGTTGCTGCATTAACGCAAAACTGTGTACGTGCAATTCCATTAGGACAAGTTAAGGGACAAAAAATAATTTATCAACTTAAACATGTTTATTTTGATGATATTGTTAATAAAGTCTTTACATTGGATTTTAAAACAGATTTTTGCAAAAATATACCAGGCCTTGAAATTGCACAAATGGAACACGAAGACACACCTGTTCGATTATTCATGTCATAATTAAAGTTTTAGTCAACATTACATTAATCATAAAAATACCATTTAATATTTTTAAAAATTAAAAAAGAAAGAGAATTAATTATGAAAAGACCATTAATTATTGGTGTAGGTGGACCTGTAGGAGCAGGTAAAACAATGCTAATCGAAAGATTAACAAGATACCTTTCAACAAAAGGTTATAGTATGGCAGCAATTACTAATGATATTTACACTAAAGAAGATGCTAGAATTTTATTAAATACTTCTGTTTTACCAGCTGATCGTATTGCTGGTGTTGAAACAGGAGGATGTCCACATACAGCAATTCGTGAAGATGCTTCAATGAACTTTGCTGCAATTGAAGAAATGTGTGATAAACACCCTGATTTACAATTATTATTTTTAGAATCTGGTGGGGATAATTTATCTGCAACATTTAGTCCAGATTTAGTTGATTTTTCAATTTATATCATTGATGTTGCACAAGGAGAAAAAATTCCTCGTAAGGGTGGTCAAGGAATGATTAAATCAGACTTATTTATTATTAATAAAGTTGATTTAGCTCCTTATGTTGGTGCTAATGTAGAAGTAATGAAAGCCGATACATTAAAATCACGTGGTAATAAAGATTTCTTTGTAACGAATTTAAAAACAGATGAAGGTTTAAAATCTGTTGCTGATTGAATTGAAAAGCGTTTGCAATTAGCTTTACTAGAAGAATAAAGTTAACAAATGATTTTAAATAAAGAAAAAATTAAAAATTATGCTGCTTATTTATACATTAAAGTAGCATATGATCAAGCTCATTCAAAAATGGCACATACTGTCTATTTTACGAATTTTTATCGTTCATCTAAACCTTTATTTTTAGATGAAGAAGATCCAATCAACCCCTGTTTTCAAACAATTAGTATGGGTGGTGGATATGTTTCAGGTGAAATTTATCGTTCTGATTTTGAAATTAATGATGATGCTCGTTGTATCATTACAACACAATCCTCAGCTAAAGCTTATAAAACGGTTGATGGAAAAACTTCTGAGCAACATACAAATATCACGTTAGGAAAAAATAGTATTTTAGAATATATAAGTGATAACGTAATTGTTTATGAAGATGGAAAGTTCGCACAATTTAATAATTTTAAAATGGATTCAAGTGCTACTTTAATTTACACAGAATGTTTTGGCCCTGGTTGATCGCCACATGGGTCTGCTTATCAATACGAAAAAATGTATTTAAATACTAAAATATATTATGATGATAAATTGGTTTTATTTGATAATTTAAAATTTCAACCACGTAAAAATGATGAATCAGCATTTGGAATCATGGATGGCTATCATTATTGTGGAACCATGATTGTAATTAATCAACAAGTTATTGAAGATGATGTTATTAAAATTCGTGATTTAGTTAAGGAAAAATATCCTGACATGGATATGATATTTGGTGTATCACGTATGGATATTCCTGGTTTGGGATTACGAGTTTTAGCTAATACTTATTATCATGTTGAAAAAATTAATGCTGTTGCTCATGATTATTTTAGAAGAAAATTATTCAATAAAAAACCTTTAATTTTACGAAAACCATAAAAGATTTAAAAAACCTTAAAACGTTAAGTTTTAAGGTTTTTATTTCTTATAAATTGTTAAATAATTATATAATATATATGAATTAGACCACAAGGAGGATTTTTAAATGGTTAAATCTCAAAAAGTTATAGATGTTTTAAATACACATTATAATTTAAATTTAGAACTAGGTAGTATTTATGCACAATATGCTCATATTGCTGATGACCAATTCAGTATGCCTTTTTTAGCAAAATTTATTGCTGATTTAAGCAATGATAAATTAGGTGTTCACAAGAATTTAATTTCTGAATATGCTCGTAAAGTTGAAATTCCATTACACACTAAATTTAGTGTAGATGTTAATTTTAAGCCAGCGAATCCTAAGGAATTAATAAAACACATTTTAGATACAGAATTAAAAGTTCGTAAGCATGTAGCCAATATGGCAAAAGTATGTTTGGAAGAAAATGATTTTGAAACATTTAGTTTTGTAAAATGATTTGTTGATGATGGTATTAAAGATTTTGATGATGTTCGTACCATCCATGATTTCTTTGAAAATGCTACTAATAATTTGCAAGTTGAATATGCCATTCGTAAATATTTAAAGCAAATGAAAGTTGAAGAAGAAAAATAATTTAATTATATATTAAACAATAAAAACCATTTATAAGGTTATAAAAATACTTATAAATGGTTTTTAATTTTCTATTAGTTTTTAAACTAATTGATTATTAATAATAAAATCTAAATTGTGATATTTTGGATTTAGAAATAAATGTGGATATTAACTAAATAAAGAATATAATTTAAAATTATTTAAATAATCTTTTAATTGTCTTTGATAATTAAAAGAATTTTTTTAAAATTTTAGCAAAAATTAATTGTCCATATATTCTTTCTAAAATTTGCAAATTTTCGTTTGTATAATCTTTATTTGGTTCATAATCACGTCTTAAAATGTCATAATTTTTACCAAGTAAAAAATTATCAAAACCTATAATTGATTGGGCTTCATCATTTGTTAGTTTAGTATTATTACGAATAAAATCAAAACTCGTTAGAAGTGCTGATGTTGTTGGTTGAAAACCCCATAATGAAAAATTAGGTGTGTTTTTGTTTTTAAATAAAACTGCTTTGTTATTTTTAATTGATAAATAATCTTTTAATAAGATATTTTCTCATGGTGTTTTTGCATCTAAAATAATTGGTTTAAAGATTCCATTAGTGATTTCTTGAATGTAATCTAATACTTGATTTTGATTATAAATAGGGTGTAATTTAAAAAAGTAATTAGCATTAGGATATTTGTTTTTAATTTTTAGTGCTCATTGTTGTAATTCTTGTTTAGCAATTTTACGATCTTTTAAAACATTATTTTTAAATAGTGAAGAACCCATAAAAATTAAGTTTTGCTTTTTAGGATCATAGTATTGTTTACCATCAATAATAAAATCTAAAAATGTTTTATTGTATATTCTAAAAGTGTGCTCAAAATTTTTTTTAAATTGATTTTTAGCATCTAATGATAAATTTAGTGAATTTAGTACGCTTGCATAATTTGTGCGAATTTTATAAGATTTAGGCATTAGTTCCTTATTAAAACTAAAGTACTTAGAATCGACTCAACTTTCACCAATTGTAAAAATTTTAAACAACTTATTTAAGACAAAAACTGTTAATCAATCTTTATTATTATTTAATTGTGAACTAAAATAATTTTTTGCTTCTAAAAATGATTTCATATTAATAAAATTATTTAAATGATCATTAATCTTTTGTTTTGATAAATGGGGATCTTCCCTAATTAAATTTTTAATTGCCTGTGCGTAATAATTTGCAACATAATATAATTCAGCATTTCCATTCGGGTATAAAATTATTTTATTAGCATTATAAATTAGTTTTAAAATAACATCTAAACGATTTTGATCAATTCAATATTGATAACACCAATGGCTTAGATAAAAATCATATTTAATATCTTTAAAAATACTTAAATGATAATCAACATTTTCGCTTAGTCAATTATTATTTTTAACAAAATCTTCTTTTAGTTTTTTATTATCTATTTGGTATTTAGGTGTTTGTTTTAAATTAGAATAAATTAGTAAATCATTGCTAGTTGCCATTAAATTATGTTCATTATTATTCGCATATTGGTTAATAACAGATATTCAATTAACTCTTGTATCCTTAGAAATTGCTTGGTTTGTTAAATATTTATTAGAATCAAGTTCAATATAAGGAACAAAAGTAATAATTTTATTAGTTTTAGCTTTAGAAAAATTTTGATTATTTAAATTGTCTTTTTTAGCTTCTATTTCACCAATTATTGCATTAAATTGTTGTAAATATGCAAATGATGCATTTCACTCGTTGATGTGAAAAGTTGGATTTTTGTTTTTTGCTGAATCAAGAATTTGTTTTTATCGCTCTGTTGCTTGTTGCTCACTAAACTCAAATGAGTTTTTCGTAATTCATGTTTTATTATTACAAGCTGTTGCAACTATAGTAGTTTGAATAATAATCATTGTGAATGATAATGATGAAAATAAGATTTTTTATTTATTTTTTTAAGCACGACTTATATCCTCTAAGATGAAAAAATAACTCCAAATTGTATTTGGAGACACTATGTTAACATAATAATTAATATACTTCATTTTGATCTCAAAATGCTTGCCTAAAATATTCTAATTATTTGTTTATTTTATTTTAACAAATTTAAATAACTTTATACTTGCTCTTCTTCTAAAATTTTATTAATACAAGCCACAATAGCTTTTTGTTCTTCACTATACTGACAAACATGATTTTCCATAGCATTTTTTACATAATCAATAATTGTTTTAGCAATTAAATTATGATTATGTGCAAATTTAAATGGAAAATAATATGTAATGTACATCATTAATAATTTATTGGCTAAATGTAATAAAGAAGGATTTTTAGCTAAATACTTATTTAGATAATTTTGAATTTGGTCAATTTCTTGGTAATGGTTATTAAAATTAACAGGGTTAACATTTTTTGTTTGTTTTAAATATTTATTGTAAAAATTAAAATTTTGATCAATATTATTAGTTGCTAATAAATCTAAAATATCTAATTTTGTTGATCCATTAATTAATTTATTTGTTAGCAAGCTATTTAAAAATGTAATATCTAATTCATCCATTTCATTAATGAAACGCATTAAAAAAGTTTCTAAATATGCTAAACTAACATAAGTTGTTTTTGGATCATGAATTTTACAATAAAAATCTGTTTTAGCTAATTTTGCCTCATTTTCATCAATAAAGTTAAGTTCGGCACTTTTTTTAATTTCTATAGCAAATGCTTCAAATTCTTGTAAAGTTTTTAAATCAATTAATGGATTATTAAATTCATTAGAAATTAAATCATATGCATACGCATAATCATGTTTTTTTAAAACCTGTTTAGCCTGTATTAAAATATCATCAAAATATTTTTTAATGTAGCTTTTATTATTATTCATTATCTTTAACATTCCCTTTTTTATTTTTAAAATGATGCAAACCATTTTTTTTATTAAAATGTTTTTTTAAATTATAATTTCATTCATTAGTGTGTTTATTAAAATTAGTTTTAAAATCATCAATATCATCTAAAACGTGTAGTTTAATCAATAATTGACGAGTGACAAGTTTACGAAAATAACCTTCTTTAATATAATTTTCAATTTTAGTTTCAACGCTTAAAATTTTTTGAATCAAATCTTCTGAATAATGATATTTTTTGGCATTTTCAATAAATTCATTAACATCAACTTCTTTTCATTTATCATCTGATGAAATTTTAAAATCTAAATCAAAATCATAATACTTAACTGCTTGCTCTTCATAAATAAAAGGAGATGCAACATTAATATAAAACTTGATACCATCATTTTCAACCGTTGCAATTAAATTAAACCACTCATTTTTAAAAAAGAACCAATAACTCATCTTATTTGTAAATGATGGAAAATTACGAACAGATTTTTCTTCGCTAGTAATTACTAATGTATTAGTTGAAGATAAAATTAATAATTGATCATCAATATAAACAACTTTTGGATTATTTCAAGAACGATACAATCAACCATTAAGTTTATAAGCATGCACCATTAGTTCATCACCAATTTTTAATTGATCTAGATCTTGAAAATTTTCATTCTTTGTTTGTTTTTTTTCTTGCATATGTTTATCCTTTATCTAATTTGTTTGATAACTAAATTAATTAAAATATCAACATTAATATGATAATATTCTTGTAAAATTTGCATACAATCTACAAATGCTTTATTGCGTAAACAAATAATATTAAAATCATCAATTTCATAATTTATTGGAATATTAATGTTATACCAATAAAAAATTATGTATTTCAAATATATTTGTTCCAATTGCTTATTCAATAAAAAATTACTAATATTTAGTACTTTTATTAATTCTTTAATTTCTAAGTATCAACAATCTAAATCAAAATTTTGTGATTGATTATTTTTATTAATTATTAAAAAAAATCGATAAACATAATCGCTAAATTTTTTTCAACTATCTAATGATATTTCTTTAAAAACCATTTTAGAATTAAAATAAATTGTTGCAATTAAGAATTCTAATTCTTTTTTATATTCATTTAAGTTTTTAATTTCTTTTTTAATCAATTCGTAATGAGTATTAAATTCACGTTTAATTTTTTTAATGTTCATTTGTTGATCCTAAAATTTTTTCAATTTCAATTTTTAAAAAATCTAATAAAGCTTTGATTTTTGCATAATCCATTCTTGTTGGACCTACAACGGTAATTTGATGATTAGTGTTTGCTAAATTAATTTGAGTTGTTGCAATGGCTAAATGTTCGTGGCCAATTTCATTTCCTAGCATAATTTTTGCTAACCCATCAGTTTTTTTGTTTAAAGCAATTTGTTGTCAAATTGTACTGTCTTCTAATAAATTTAGTAAATCATTTAGTTTTTGTTGATCTTCAAATTCAGAATGTTTAGTTAAGTATTTAACTCCATAAGTTTTAGAATCAATGTTAGGTTTATAAGCGTAAAAATCAAAAACACGATGTACAATTTTACTAAAATAGTATTCTACTTCTTCAAGTTTATTACGAACTATATCGACAATTAAATCTAATTTAGAACTGATTAAACTAAGTTTAGTATCAACTAATCGTTCATCTAAAACACTAAAACACGTTTGTAAATCATTAAATTTTGTGTTTTTATCAAGCTTAATTGAATGAGTTAAAATGTTTTCGCTTGAAGTTACAATTAACACTAACGCCGAATTATCATTGATTTTTACTAAATCAATTCGTTTTAATAATTCATCACTACTTGTTTTTGTAACAACAGCTGGTAATTGGGTTGTTTCATTAATGATACTTACAGCAGCATTTAAAATTTCATCAATATTGGAATATCGTTTACTAAGGATTTTATGTAAACGCATTTTAAAATTATCAGAAACATTATTATTTTTATTAATTAAATTTTTATTATAAAAATCATAACCTTTTAGTGATGGAACACGCCCACCACTAATGTGCTGTTTTTCAATTAATCCGTTTTTTTCAAGAAACATCATTTCGTTACGAATTGTAGCAGAAGATTGATCTTTGAATTCTTTTTGTACTAAAAGTTTTGAAGAGACCGGTGTTGCACTAATAGTGTATTCATCGACGATAGATTTTAATACTTTTTTTTGCCGATCACTTAATTTTAATTCTATTACTTGTTCTTGCGATGTTTTAGTATTCTTCATTAAGGAAATCTCCACCTTTAATTCATAATTCTTCAAAATCATTAATAATTGTACCTAGTTTTGATTCAATTTGAAAAGCGATTGATTCAATGTTATTCTCAAGATAAAACTTAGAAAAACCAGCATTTCATTTTTCTAACTCATAAATTTTTAAATCATAATTTCCTTTTAAGTCTCATTCTTTAGAACTAATTTTATTACCATTAGTTCTTTTTATTAATCATACTTGTGGTTTTTGTTGGAATGAATAAATTAAACTATATTTTTCATTACAATAATTGATATCAATAACTTGATTACTTTGTTTAAAATTTAAAAAACCTTCTGTTAATAACCTAAATTCTTGAAAAAAAGTACTTATTTTTTTAATTAGTTGTCCAACATTAACTTCATTAAAATGATATTCAATATATTTATGCATAATTTTTTTATTTTGATTTGCAACAGTAGTGTAAGGAATTAATAAACATAACAAAAAGAAAATTCCTATGATAACACAAGTTATTGCCCCATTATTTAAATTAGCGCCATTACCTTTATTAATAAAAGCACCAAGAATTAAAATTCCCACACCAATTATAAAAATGATAATTAAACTAATTAATCCAATTAAACGATTTTTTAATAATTTTTCTTTTAATTTTTCATCATTAAAGTATTGTTTAATAAGATCTTGAGTTTTTAAACTAATACAATATTGATTAAATTCTTTTTTTAAATTTATTGTTTTCATTTTTGAACTTTCTTTTTATTAAATATGATTATTCATTTATACATAATTATAAAAAATAAATAATTATATAAATTATAATTTAAAGTTAATGGATCTAAAATAAGGAGTTAGTTTATGCCATTTATTAAAACGCCAAATAAAGATTTTGAATTAGTTGATAACCAAATTGTTTTATCAAGCGATTATAAAAAACGTTATCAACGATATTTTGCAAAAATTACAGGTAGTAGATTAGGTTCTATTTTAAATATTGGTGATTTTACTAATCCAGTTCGTACCTGAGCAATGATGGTTAAAATTTATAATGAACCAATCGATCCAATATACGCTCAAGCCGGTGTTGTAATTGAACCAAAAATCAAAGATTATGTTGAGACTGTTTTAAAAGTTAAATATAAACAATACGAACCTGCGCAAATTGGTTATGATGTGTTTAAAGATAATCAAATTTTTGGTGGAATTCCGGATGGTGAACCAATTGATGAAAATGGTCAATTATTGTACCCAAATCAACCAATGTTAGAAATTAAAACAACATCTATTGATAGTTTTTCATTTAAAACAATTGATTATGTTCTTGTGCTACAAAAAGATGAACATAATCATCCGATTATAAAAAAGAAAGGCGATAAACGTGCAAAATGATTTAATAATGATGAAAGTGAAGTTATTATTTCAGATGAATATCAATTACAATTAGGATTGTATTGTTATTTAAGAAAAATTAATAAAGGAATTTTTGCTATTGCTTTTTTAACAAGTGAAGATTATATTAATCCGCAAGCATTTGATATTAATAAAAATGAAATTATTTTAGTTAATTATGAGCTTGATTTAAATGAATTTGAAAAAGTTATTTCAAAAGCAAAAACATGATACGAAGAGTATATTATAAAAGGTATTAGTCCTAAATTAACTAAAGAAGATCTTGAATGATATAAGGTGTGAATCAATAAATATGAAACCAATAATTTATATTAAATATGGGGAATTAACACTAAAAGGTAAAAATCGAGCACAATTTATAAAAGTTTTAGTGCACAATATCAAACAAATATTAATACAATATCAAAATTTAACATACTGTGTTGGTTATGATAATTTGAAGATTATCAATTTAGAAAATTATAATTTAAAACAAATTATTGATGATTTGAAAGAAGTTTATGGAATTGCTTTTATTTGTATTGCTTATCAAGTTGATAAAGAACTTAGTGAAATTCAATTAGCTTGTAAAGAATTTATAAATGATTATGAACAAACATTTAAAATTGAAGCCCGTCGTAGTGATAAAAGTTTTAAATACAATTCAATGGAAATTAAACAAATGTGCGCTGCTTATTTATTGCAAAATTCACCCCTTTTAAAAGTTGATGTTCATTGTCCACAACTATTAATTAATATTGAAATAAAATATGATTGTGCTATAGTTTATGGTCATAAAATTATGGGTGCTAAAGGATTGCCGGTAGGTATTAATGGTAAGGCCTTAGTGTTACTGTCTGGAGGAATCGATTCTCCTGTTGCTTCTCGTTTAATTATGAAACGAGGAATTAGTATTGATTTTATTACATTTATTACTCCGCCGCACACCTCACAAAAAGCTTTAGATAAAACAATTGCATTAGCAAAACAAATAACATTAGATAATAAATTAACAAAAGCGAATCTATATGTATGCAATTTCACTAAATTACAAGACGAAATTGCGCACATTTCTAAAGAATCTTATCGAATTACACTTATGCGTCGTTATTTTATGCGTATTGCTAAACGTTTAGCAATAAGTGTTAAAGCAAATGCTTTAGTAACTGGGGAGGCACTTGGACAAGTTGCTTCACAAACTTTAAATAGCATGCAAACAATCAGTAGTGTTTTAGATAATTTTTTAGTTTTAAGACCTTTAATAGCTTACGATAAAGAAGAAATAATTAGTTTAGCTAAACGGTTTAATACTTATGAATTATCGATTTTACCATACGATGATTCATGTAGCTTGTTTGCACCTAAGAATCCAACAACCAATCCTAATGTTCAAACAGCTGCTAAATTAGAAGAAGAATCATTAGTTTTAGATGCAATTTATGAATTAGTTTACACTAAAGAAATCACTAAAATTAATTTATCATAAAATGGGAGAAAATTATTATGCAAAAAGATTTGTTAACAAAATTAATTGAACAAACATATGGTTTTAATAAAATCACTATTTTTGTTCATACAAATCCTGATTGTGATGCTTTAGGTTCTGCTTTCGCATTAGCACGAATTTTAAAATTAAATACTTTTGGAACAAGAGTTAAAATTGTTGGGGTTAACGCATTAAATCCTAATGATTTTAAAAATTTTTTTACATTTGATCCCAATGAAGTTGATGATGAATTTATTAAAGATTCAATAGCTTTTATTGTTGATACAGCTAATCAAGAAAGAGTTTTATCACAAAAACATAGATTAGCAAAAAAAACAATTTTAGTTGATCATCATGTAAAAACAATAACTTATACTGATCTTGAATACATTAATGATCAATCAATTGCAGCTTGTGAAATGATAGCATATTCATTAATGCATACTAATTTAAATTTTGATTTAAAAACTTTAAATTATCTTTTATTAGGTATTACTACTGATTCAAATCGTTTGATGTACGATAAAGTAAGTGATTTTACATATGAGATTATGTCTTGATTTTTTAAAAATAATGTCAAACATTATCAAATTTATCAACAATTGTATGAACGTAATTTAAATGATATTTTATTTGATAATGAATTGGTTAAAACAATCAAAACACAAGGACAAGTTGCATACTTAAACATTGATCCGCAATGAAATCAAAAATATCATTTTACAAGATGAGGAGATAAAGTTTATTTATTAAGTAATATTAAAAATTATCCCATTTGATTCATCGTATATTTTGATGAAAATACAAATACTTATAAAGTCTCATTACGAAGTAATAAGTACAAAGTACGTTTGGTTGCTAGTCAATTTAATGGAGGCGGACATGATTTGGCAGCAGGTTGTAGTTTAACTAATATAGATCAATTAGAAAATTTATTAAGTGCTCTTGATGCACTAATTAAAAATAAAATAGTTGTTGATTAAATAAAAATATAAGCCATTACCTAATTTTATTTATAATATTAGGTAATAAATTAATAAAATTTTTATAAGGATGTGTAATAAATTATGAGTGATAAAAAAAGTGATTTTGAAAAGTTATTTGAGCGTTCAAAAGTACAAGAAACATCTATTTTCGATATTAATGATATTGATGAACAAAAACCGTACGAAGAACATTCGATTGAAGAATTATCATATATCTTAGAATATGAAAAAATTGATAAGAAAACACGTGAAAAGATCAAAAAAATTATTAAAGAGAAACAACAAAATTTATAATGTTTATTAATTTAAATGTGCATTCACATTATTCTTTACTTAATAGTACTTTAAGTATTGATGATTTAATTAAATACGCATTAGATAACAAACAACCATACGTTTGCTTAACTGATTTAAATAATATGTATGGCTGTATCGAATTTTATGATAAAGCAAAAGCACATAATTTAACTCCTATTATCGGATTAGAATTTGAATATCAAAATGCTACTTTAGTAGCTTATGCTAAAAATTATAATGGCTATTTAAAATTAATCAAATGATCATCTTGAATTATGACAAGTAAAGAATTTGAAATTCAAGATGATTTTGATGACTTAATTATTGTTTGTAAAAAAGGAACTATAGTTTTTAAGAGTCCTAATTTTTATCAAACGCATGATCAAAATGCTCCTAATGCGATTGCATTACAAAGTGTTTTCTATGCTAACAAAAATGATAAAATTGTTTTTTTAGCAATGTTAGCAATCAAAAATGATTTAAAGTTAGAAGATTTTAAAAATTGTTGCGATTTTGATAATAATCATTTTCTAAATGATAATCTAGCACAATCATTTTTTTCACCAATTGCTTTAAATAATTTAAATAAAGTTTTAAATGAATTAAAAGTACAAATTCATGATTTACCAATTAATATTCCGGTTTATGATAAACAAAATTCAATTATTTCAAGTGAAATTTTAAAACAATTATGTATTAGCGGATTAAAAAAACGCTTAAATGCTAACGATGGCCAAGTTAATAAAATTTATGTTCAAAGATTAAAATATGAATTAGATGTTATTAATGAAAAACAATTTGATGATTATTTTTTAATTGTTTATGATTTTATTAATTTTGCTAAAAGTAATGGTATCATTGTAGGGCCAGGACGTGGGAGTGCTGCTGGTTCGTTAGTAGCTTATTGTTTACATATTACAGATATTGATCCTATTAAACATAATTTAATTTTTGAACGTTTTTTAAATCCTACCAGAAAAAGTATGCCAGATATTGATACTGATATTATGGATGAAAAACGCGATCTAGTTATTGAATATTTATTTGAAAAATATGGCAATGATCATGTTGCTTACATTTTAACGTTTCAACGTTTAAAAGCGAAGATGGCTATTCGAGATGTAGGGCGGATTTTGGGAATTGATTTAAAAGTAATTGATAAAATTTGTAAAAATATTAAACCTGAATATGATGAAGATCTTGATTTAGCAATTAAAAAAAATACGATTTTAAAAGAAATGTATCTTTTGCATAAAGAGTTGTTTGAAATTTCTAAAAAATTAATTAATGCTCCTCGCCAAATAGGCACACACGCTGCAGGGATAATTTTAAGTGATAGTTTAATTTCAAATATTATTCCAATTCAACTAGGTATTAATGACCGTCCTTTATCACAATATTCAATGGAATATTTAGAGCGTTTTGGTTTAATTAAAATGGATTTATTAGGTTTGAAAAATTTAACAATTATTGATAATGTTTTAAAAATGATTTATGAAAATCAAAATAAAAAAATTGATCTCTTTAACATTAACTATAATGATAAATTTGTCTTTGAAGATTTAGCTAAAGCAAGGACAAACGGAATATTTCAACTAGAATCGCCCGGAATGAAAAAAGTACTATTAAAGGTCAAACCTCAGAATATTGAAGATATTTCTATTGTTTCAGCTCTTTTTCGTCCTGGTCCACAACAAAATATTAAAACATTTGTTGAACGTCGTTTTAAACGTGAAGAGTTTAGTTATTGAAACGAAGCCACAAGGAAAATTTTAGAACCAACTTATGGAATTATTGTTTATCAAGAGCAAGTGATTGAATTAGTTAAAACTATTGCAAATTTTGATATTGCTACTTCCGATAATTTTCGCCGTGCAATATCAAAAAAAGATGAAAAAATTTTAATCCAATTAAAAGATGATTTTATCAATGGTGCTTTGAATAATAATTATAAACAACCATTGGTTAATCAAATTTTTGAATATATTTTTTCGTTTGCACATTATGGTTTTAACCATTCACATTCACTAGCATATTCATATATTAGTTATTGATTAGCGTATTTAAAACATTATTATACATTAGAATTTTTAAGTGTATTATTATCACATACTAGTGCATCAAAAGATAAATTATTATCATACTTAAATGAAGCAAAAGAATTTAATATTAGTATCAAGGGCCCTGATATTCAATATTTTAGTAATGATTTTGTTATTGATACTCAAAAACAAATAATTCGTTTTGGTTTCAAAACAATTAAGGGTTTTGGTGATGAATTACTTAAAAAAATCAAATTTGCTCTGCAAAAATCAACTTTAAGCGATTATATTTCATACATTGATGCTTTAAAAAAAGGCAATGTTAGTTTAAAAAACATTGAAATTTTGATACGAGTCGGCACTTTTGATAGTTTTGGAATTAATCGTTTATTTTTATTAAATAATTTGAATGAAATTTTTGAAAAAACAGGATTGAATGGTCATTTTTTTGATTTAAATTTAGTGGGTTTAGATTATGCAAAAGATATGAGTGTTAATGATCGTTATTTAGATGATGAAATTCAGTATTTAGGAATTGATTTGAATAGTTTAAATTACGCAAATTATAAAACTGAAATTGATTATAATAAACTAAAATATACAATTAAAGACTTTTATGAAATAAACACAAATTATGAAACAAACATTCTTGCACAAGTTTTAAACATTAAGCAATCAAAAACTAAAAACGGGAATGATATCTTTTATTTAGAAACACTAATTGATAATAAAAAACAAACACTAACAATTTTTCAAAATAGTAAATATTTAATTGATGAAATTAGTATTGGAGGTATTTATGTTTTTGGAGTTAAATTATTAAATCATTTTAATTTTATTGTAAATATTAAGCAAAGGATTTAGATTATGAAAAAAGCAATTGTTATTGATGGAAATTCATTGATTTATCGAGCCTTTCACGCTACTTATAAACAAGCTGAATGAGCAGTTGAAAAACAATTAATGCCTACTAATGCCATTAAATTAGTAGCCTCAATGATTTTTAAGATTTTAAATCAAGAACAATTTACTTATGGTTTAATTGCTATGGACGCTTCTAAACAAACTTTTCGTGCTCAAGAATATGCTGCTTACAAAGCGACACGTAAACCTATGGATGCAAAACTAATTGTTCAATTACCCTATTTAAAAAAACTATTCGTTGCTATGGGTTTTCGAATAATTTCACAACCTGGAATTGAAGCTGACGATTTTGTTGGTTCTTTTAGTAATTTAATGAATAAGTTTGATGTTGATACAACTATTTATAGTTCAGATCGTGATATGTTGCAATTAATTAACAAAAACACAAAACTTAAACTCTTAAAAACAGGGACAAGTGTTATGCAAGAAATTAATTTAACAAATTTTTCATTATTAAATAATGGCATTTTACCGTATCAAATTATTGAATATAAGGGTTTAGTGGGTGATAGTAGTGATAATTTAATTGGCGTGAAGGGAATCGGCCCTAAAACAGCTATTAATTTACTTTTAAAATATGATAATATAGAAAATATTTATACTAATTTAGAAAAAATAGCACCTAGTGTTAAAAATAAATTAATTGAACACAAAGATATGGCTTTTTTATCAAAAAAATTAGCGGCGATTCAAACGGATTTATTGTTTGATGAAAAATTAGAAAATTTTGTTTTAAAACCATATAATACTCAAGAATTAGATGAGTTATTCACATCTTTAAAAATAAATAATATGCAAAATTATTATAAATAATTTATCCATATTATAATCTGCTATATAAGGGGGTTTAAAAAATGCCAGAGCTACCAGAAGTACAAACGATTGTTGATTATTTAAATCATCATGTTCTCGATATTTTTATTAAAAAAACTATTGTTCATTTACCTAAAATTTTAAAAAATAAAACACCACAAGAATTTGAAAAACTGTTAATTAATCATAAAATAGTCAAAATTAAACGTCTTGGTAAATATTTATTATTTTTTTTAAGTAATAATTTAGTATTAAGTGTACATTTGCGGATGGAGGGAAAATTTTATTATCAAGCAAAAGAGGAATGATTTAATTTAGCACATACTCATATTATTATTGAATTTAATAATGGTATGCAATTAAGATATAATGATACGCGTCAATTTGGAACTTTTCACATTTATGAGCAACAATCTTTTTTAGATTCAAAAGAATTAAAAAAGATTGCTTTAGATCCGCTAGATAATAATTTTTCAGCGCAGTATTTGTATGAAAAATTAAAAAAAAGTAATAAAGCCATTAAAACAGCCTTGTTAGATCAATCTGTTGTTTCAGGAATTGGTAACATTTATGCTGATGAAATTTTATTTGCAGCTAAAATTTTTCCTACAATATTAGCTAAAAATCTTACTTTAAAAAATTATGAAAAGATCACAAAAGAAGCACAACGAATCTTATTATTATCAATTAAAAATAAAGGAACAACAATCCACACTTATAAATTTGGTAATGATGAAACAGGTTTGTTTCAAAAGATGTTATTAGTTCACACACACGCTAAAGAACCATGCCAAATTTGTGGAACAATTATTCAAAAAACAAAGGTCAACGGTCGGGGAACTTATTATTGTCCAAATTGTCAAAACTAAAATTAATTAATCTTTTACTAGTATTTTAAATTCAAAATTGTTATAATAATTAATGTTTAATTTGCTCAAGTGGCGAAATGGCAGACGCAGTTGACTCAAAATCAACCGAGAAATCATAAGGGTTCGAGTCCCTTCTTGAGCACCATTATATTATTTATTAAAAAAACGATATGCCCTTTATTGTGCGTATCGTTTTTTAAATTTATTTACTATATTTTATTAAATCTTGAACCTTATTTAATTTTTCTCAACTTAAGTTTAAATCATCACGACCGAAATGACCATAAGTTGCTACTGGTAAATATTTAGTTGTTCACAATTCTAAATCATTAATAAACGTTTTGATATCAAAATTAAAACTTTTATTAATTGCTTCAAAAATTTTCTTTTCATCAACCAAATTAGTCTTGAATGTATTAACAAACATTGCCACAGGTTGTGGTTCTCCAATTGCAAAAGCTAATTGAATTTCACACTGTTTAGCAAGCTTAGCTGCAACAACATTTTTAGCTATTCAACGTGCAAAGTATGAAGCACTACGATCAACTTTAGTAGGGTCTTTGCCACTAAAAGCACCACCACCATGATGACCTATTCCTCCATAAGTATCTACGATAATTTTTCTTCCAGTTAAACCAGTGTCACCAATTGGACCACCAATTACAAATCTCCCTGAGGAATTAATAATTTTTTTAAAATCGGTATTTAAACCATATTTTATAGCAATTTGATCCATAATTGTGCTAACTTTTTTATTAAAATATTCAATGTTATAATCTTCATCATGTTGAATACTTACCAACATTGTCTCAATAACAGGCGTACAATTAGAATAATCAATACTTACTTGTGATTTCATATCAGCTTGAATTTTAACAAATTCTTTATTACGTCTTTGTTTTTCGATTTCTTTTAGTAATTCATGAGCTAACACTGTTGTTAATGGCATATATTGAGGTGTTTCATCACAAGCATAGCCAAAAACAATTCCTTGGTCACCAGCTCCAATTAAATCTTTATTTGCTTTATTAACACTTTGCGCTATATCTACTGATTGTTTATTAACATTAGAAATAATCGTAAAATCATTTTCATTATAGCCTAAAGGTTTAATAATTTCTCAAGCGGTTTTTACTACATCAACATATGCATGCGTGGTAATTTCCCCAGCAATAACAATTAAACGATTACAAGCTAAAACTTCACAAGCTACACGTGAATTTTTATCTTGTGCTAAGCATTCATCTAAAATTGCATCACTAATCTGATCACATATTTTATCTGGATGGCCTGCACCAACAGATTCGCTCGTTATAATTTTTTTATATTTCATACTAAATTCTTTTCTTTCTACCATTATTTTATTAATATTGATTTCTTATGATTTTTAATTAAAGCATAAGCATAAGCCCCAATCATAGCAGCATTATCACCTGTATAAATCATTTCTGGAATATATGATATAACATCTAAAGATTGAATTTTTTTACGGAGTAAACTATTAGCTGATACGCCTCCCCCAATTGCTAAATGATTTAATTTGTATAAATTTAAGTAATATTTAATTTTCTTTATAATTTCATTAATTGCAAATTTTTGAAAACTTGAAGCAACAACAACAGGGTCAAAACTTATCTTTTTTTGTTTAGCATTATGAATGTAATTAATTACAGCAGTTTTTAACCCACTAAAACTAAAATCTTTAGCAGCGGGCTGTGATTTAATAAATTCTAATGTTGCTAAATTTTCTTGATAATTTTTATCAATAATTGGACCCCCTGGATATTTTCAACCAAGAACACGAGCAATTTTATCATAACATTCACCAATTGCATCATCGTGTGTTTGATTTAAAACTTTGATTTCATCATAATCATTTACTAAATATATACAACTTTCCCCTCCTGAAACCACTAATCCCAAAAATGGAAAAGTCAAATTTTGATTAATACTTGCACTAAAAACATGCGCATGTAAATGATTGATTGGCACTAATTCAGCATTAATTAAAACAGCTAATTGTTTCGCAAAAACTTTTCCTACATGTAAACAACCTGGCAAACCAGGCATTGCAGTGTAAGCAACGTGAGTGATAGTTAAAGGGTTGATTTTTGTTTCATTTAAAATTTCATTAAATAGGTGATTAATATTTTGTTCATGATAACGACTTGCTAATTCAGGAACAACACCGCCATGAAGTGATTGTATAGATGCTGAACTACTAATTTTATGAGCAATTAATTTATTATTTTCAAATAACGCTAAACTTGTTTCATCACAACTTGATTCAATGCTTAAAATTAAATAATTATCATTCATGTTCTATTTTATTTTATTAATTACTTTATAACAATTCATTGATAATTCATCATTGTACATTTCTTCATCAGGGAAATGATTTCAACAACGTAAGCACTTTTTAAAATTAGCATTAGCCACATTTGTGTTATTAGCAAATTCAATCTTAGCAACACCAAACCATTTTGCTAATAGTTTTGGATTATTTATCAAATAATCATCTAAAATCAAATTTTTTGCAATTGTTACAAAAGCTTCATTATTTTTCTTTAAAATATTATTTTTTCGTGCATTTTCAAGTTCATTAAAAATTTGATCTTTAATACTAAAGAATGCTTTAACATGAGCAATGCCTTTTTTGAATTGAAATTGGGATTGATCATAAAAATCCTCTAAACGGATTGATTCTTTTTTATTAGCATCTTTTAAAAACGAGTAAGCTTCTTCAGTTGTGTGTGGAATAATTGGTGTTAAATCAATTAATGAATTTTTTAAAATTCAATAAACTGCTGATTGAATTTGTTTGCGAATTGGATCATTAATTGCCAAACAGTACATATCATCTTTAATATTTTCAAAATATCATGATGATAAATCTAAGATAAATTTGTTAAATAGTTTAACAACGGTTTGATAATCAAAATTCTCATATGCTTTAATAACTTTTATATGTAACTCATTAAATTCATTTAAAACAAATTGCTCTTCTTGACGAACATTAAAACTAAAATCAACACTTGGTTCAAAATCATTTAAGATTGATAATGAATATTTAAATAAAGTATTTCTAATTCTTCGGTAAATTTCAGCATTTTGTTTTAATATCTCTTCTGAAATTCTTGTATCATTCTGATAATCTGAATTAGCAATTCATAATCTTAAAACATCTGCTCCATAAATCTTACATACATCTAATGGATCAACAACATTTCCTTTTGATTTTGACATCTTATATCCTTCACCATCTAGAACCATTCCATGTGCTACTAAATATTTATAAGGTGCGCGATTGTTTAAAATTGTCCCTGTAATTAATGAAGAATTAAATCAACCACGATATTGATCATAACCTTCTAAATAAACGTCTGCTGGATAATTTAGGTTATAGTGTCCTAAAATATTATATGAACTACCAGAATCAAATCAAACATCCATAATGTCTTTTTCTTTATAAAAAGTTTTATTTTTCATACTTAAATACTTAGGTGTTAAAAAAGCATTTACATCTAAATCAAATCATGCATTAACACCTTTTTCATTTAAAACATTAATGATATTTTCTACTAATTCATGATCCATAATTGCATTATGATTCTCATCAAAAATCATAGGAATAGGAACACCTCAAACGCGTTGACGTGATATACATCATTCCTTACGATTAACAATCATTTCATGCATTCTTTCAATTCCTCTAAAACTAGTTGATTTAACATCGTTATTTAGGGTCTGCAAAATGTTAGGTAAAATTTTTTCAATAGAAACAAACCATTGTTTTGTGGCACGATACATAACAGGTTTTTTTGTTCTTCAATCATGTGCAACACTATGAGTGATAACTTCATGATGGATTAATAAATGTTCATTCATCAAACGATTTAAAATAACTTGATTAGCATCTTCATAAAACATATTTTCTAATTCTAAATCATTTACTAATGCATTGTATTTACCAAATTGGTCTATCATAACTACTGTTTCAATACCATATTTTTTACATGCATAATAGTCTTCTAAACCAAAAGCAGGCGCATTATGAACTAAACCCGTTCCGTCATTAGCACTAACATACTCATCCATGATCACAAAAGCATGCTTTTTACTAATTGGTGAAATATATTTTATTTTTTCTAAAGTATTAGCTTTAAAATCTTTAATTCATTTATAATCTTCAAACCCAACTTTTTTTACAAAAGTTTCAACAAGAGGTTTAGCGATTATATAATTTTCATTTTTATATTCAATTAAACTGTAAACTAATTCTGGATGAATTGCAATTGCTAAATTAGCTGGTAATGTTCATGGAGTTGTTGTTCAAATCACTAATGATGTTGGTTTATCAGTAAACAAATCATTATGATCAACTACCTTCATTTTTACGTAAATAGCTGAAGATTGACGATCAGCATACTCGATCTCTGATTCTGCTAATGCTGTTTGTGATGATCATGACCAAAAAACAGGCTTAAAGTCTTGATAAATAAAACCTTTTTTTAGCATATAAGTAAAAAGTTTTAATTGATCTATTTCGTAATTATGGTCTAATGTCAAATAGCGTTGTTTAAAATCTGATATCATTCCTAATCTTAAAAATTGTTGTATTTGATTATTAACTTGTTCTAAAGCGTATTTTTTACATTGTTCACGACGTTCATTAACACTTAGATTAGATAATTTAATTTTTTTAGATAAGGCAACCTCAATTGGTAAACCATGTGTATCTCATCCTGGAATATAAGGAGAGTAATAATTATTCATATTGTGATATGAAACAACAAAATCTTTTAGTATTTTATTTAAAGCGTGACCAATATGAATATTTCCATTTGCATATGGCGGCCCATCATGCAAGATAAAAGGCTTCTTGTCTTTATTTTTAGTTAGCAATTTTTCATAAATTTGGTGTTCAACTCAAAATTGTTGAATTTTTGGTTCTTTAACGTTTAAATTAGCACGCATTTCAAAACCTGTAGTAGGCATATTAAGGGTGCTTTTATAATCTTTCATACTCTCTTTTTTCCTTTTTTTGTTTGAATTATTTACTTAAAACAACATTACTAAGACTCGTAATAATGTTGTAATATAATTATTAAAGTTAAACATTAATTTGGTTTAATTAATACTTAGAATTATACTAAAAAATAAGGGGTGCTTGTTATTTGTCAGCAAATTATTTTTTAATTGGTTTAACTATTTTTTGCATTACTTGTATTGGTTTAGCTAATTTTCTTATTAAACCAATACAAATTAAAAAACATAATTGGTTAACATGAAATTATTTTATAAACACTAAATGAATTATTAATACTCTAATTAGCATAAGTTTTTTAATTTATTTTTGTTGTTTGCGATGAGCACCAAATATTCATGTTTTTTTTATTAATCATATTGAAATAATCAATGTTTTAAGGGATAAATTTAGTGATGATATTGATTTATATGAAGCATCTATTACAATTTCACGCACATTTTTATTAGATTGATGTCCTTGTTTTTCAGTATTAATTTCATGTGTAGCCATTTTTGATAAAAAACAGATTATTATTAATCATTTAGGTTTTTTGTGCTTTTGTTTTGGTTTGTTAACAATTATTGGTGGACTAATTGGTGATGAACCAATTAGATGAAATCGTTTAATAACTTATATTTTTATAGGGGACAAGCCTAATCCCATTTATTTTAGTTTACACTTTTATTTGTGTCTTTTTGGATTTTATTTATTTGTAAACACGCGTAAAACCATTAAAATATACTGGATATATATCATCATTCATATTATTCTAATTACTTATATCATTTATGTTAACGTGATGATATTAATTTTTAATATTAAGAATAACGCTAGTGGTTTTAGTTTTGGTGATTGATATTCTCCAATTTATGCACAATATGGTACCGTAGCACAATTCTTACAATTAAATTACCGATTAAACGCTTTAATTATGTGTTTATTTATGTATGCAATTTTTATTGTAGGTTATTTAATACGTTATCACTTGCAAAAAATTTACATAAACAAATATAATATAAAGTATAGTCAAGAGAGGAATTATTTTGCTTATGTCAAAAATTGTCGATAACATTGATATCAATGCATTAAGAATTAATCATTATGAAGAATGAGAACAAAATCATATTATTAAGGTAATTAATGCTTATCGTCATCATGAATCAAAAAAAGAAAAATTTAAAATGATTCATGAATTGATTAAAAACCACCAAATGCATTTAACGAAATTAATTCCGTTGTTTGATGTATCAATTTCTGGTTATTATAAATGACTAGAAGACTTAAAAATAGATCAAGTTTGTCCAATTAAAAAAAGAAATATGGATTTAATTGAAAAAATTTGTAAAAGTCATAAGCATTATATTGGTTGTCGTAAAATTCAAAAAATTCTTGAAACACAATATAATGTTAAATTAAATTACAAAACAATTAATTTATATATGAATAAAATGGATTTATGCAAACCTTGTCATCATGAACATCATGAAAAAAAGGAATAACGTTGTCTATTTTTTCATAATTAATTATGATTATTTTTAGCAATCAGATATGATGATTGCTAATTTTTATTATTTTAGGTTATAATATATCATTGTAATTATCATATTTAGGGGGAATGTTTTAAGTATGGCAAAACGTGATTATTATGAAATTTTAGGTGTTAGTAAATCAGCAACTCCTGAAGAGATTAAAGCGGCATTTCGTAAATTAGCAAAAGAACACCATCCTGATCGTAATAAATCTGCTGATGATACTCTTTTTAAAGAAATTAATGAAGCATATGAGGTTTTATCTGATTCTAAAAAACGTGCACAATACGACCAATTTGGCCATGATGGTCCACAAGGTTTTAGCAGTGCTAGTGGGTTTTCTGGGTTTAGTGGAGGATTTGGTGGTGTTGATTTTGATATTAATGACATTTTTGGCAGTTTTTTTAATAATGCTTCTAGTTCACATAATTCATCAAACCAATATGAAACATATGATATTCACTTACGTTTACATTTAGATTTTATGGAAGCTGTTAATGGTATTTCTAAAAATATTAATTATGATCGTAAAATTACGTGTCATAAATGTCAAGGAACAGGTGCAAAAGATCCTAAAGATGTCAAAATTTGCACTAAATGTCACGGTAGAGGAACTAGCATTGAAAATGTTCATTCCTTATTTGGAACAATTCAACAAGAAGTTGAATGTCATGAGTGTGAAGGAACAGGTAAAGTTGCTAGTAGCAAATGTGAACAATGTTATGGTAAAAAAGTTATCAATGAACGTGTTAATTTAACTGTTGAAATCCCAGCAGGTACACGAGAAGGTGAAAAATTATTAGTAAGCAAAAAAGGTAATATTGTCAATAATCAAGAATTTGATCTTTATTTACACATTAGTGTTAAACCATCAAAGTATTTTGCATTAGATGGTTTAGATATTTATTCAGAAACTTACATTGATCCCATCAAAGCAATTGTTGGAGGAATAATTGAAGTAGTAACAATTAATGGTATTAAAACCATTGAAATTCCTTCAAATACTCCTGAAGGTAAAAAATTTAGAATTAGTGGATCAGGAATCGTTAATAAAAAATCAAATATTTTTGGTAAAAAGAATGGAGATTTTTATACTACAATTCGTTATGCAAAGCCAGTTGAATTATCCAAAGATGAAATTGCTTATTTAAAAAATATTTCTACTCGTACAAACCAAAATGTTGAGTATTATAAAAATAAAGTATTAAAGGAGATTAGTAAATAATGAGCAAAAATAATGAAAACATCAAACACCAAAATAATGACAAAGTTAACAACCAAGTTGATAAAAAAGAAACTAAAAATCATAATAAGCAAGAATTTAAATATAAAGAATTATATGAACATGAATTAAAGAAAAATAAAGAATTACAAAATATCAATACACTTTTAAAAGATAAAAATCAACAGTTAGAAGAGCAAATTAGTCAATTAAATCAAGATTTTATCAAACAATTAGAAACAAAAGCAAAACAAGCACAACAAATTCTAGAACAAAAAGTTAATGAACTAGAAGCACGCCATGAAGCCAAAGTAAATGATGCTGTCTTTAAAATTTTCAAATTTAAAATGGAACCTTTATTAGATGCAATTAATCATTTTACGAAAATTGTAAATCAAAACTATGATGATCCTAAAATCCAAGCTTTCATTGAGGGGTTTAAAATGTTTTCTCAAAACATGATTGATGGTTTAGATAATCTAAAAATTACAAAAATATCTCCACAGGTTAATGATAGTTTAAATGACGAAATAATGGAAGTTTTTGAAGTTGTTGAAAACACAAACAAACCATCAATGCATGTAGTTGAAGTTATTAGTGATGGTTTTAAATATAATGATAAAGTTATTAAATTTGCAGTTGTAAAAGTTGCTAAATAAAATAAAAAAAGTGAATAGCATTTTATAAATTGCTTTCACTTTTTTTATTTATGCTCTATAAATGCATGGTAATAAAATTGCATTTAAAAAAATTAGCCAATTAAGATTTTATTATTTTTGTATTTGTTAATGAATTTTAATATGGTTCAATTTATGCTAAAAACAAGAATTTGATAACCGATTAGTGCGAATAACATTGTTACAAAAATAGGATTACCATTTGCGCTTGGTCAAATATTCGTAATTGCTCCATAAACAGTAACATTTGCTAAAAGAGGTAAAAATAGCGCATACATTGCATAAATTACTACATAAAACATAAATCCTTTTCATGCATAAAAATACTTTATGTGAATTGCATATTTATTTTTGATAATACAATAATAAGCAAAAATTGTAAAAGATATTGGAGTAATTAAATGATTATAAAAACCCACCATATTATTAAATCAAACTCAAGTTGATTGCCGTGAAAACATTGGAAACAAAACACATAATCAAATTGAACTAATAATTAAAATGTAAGACAAAACAATTAATAAAACACGATTGTTTTTTGTACAACGTGCTTTTGGATTAATAAAATATAAGAATGCAATTCCAATACACATTCAATTCCCTTGAGAAGTAAAATAATGCATATTATGAAATCAGACATTGTTTGTGTAAATAACCTTTTCGCCTAAACTATTTTTGTTAAAAATAATCAAACGCCCTGGATTCTTCGGGACATAATCAATCGATGAAAAAATTATAAAAGGAATAGCAATAATTAAAAAAACTAAAGCGAAAATTGTTTTTGAATTTTTGATATTAAATTCAATTTTATTATTTTCACTTAATACATGATAAAAACTACGTTGTCAGTAAAAAGATCATGATGTTCAATTTTTAACAATAATAACATAAGCATAAGGATTAAATAAAATTGACGCTAATGCAAAATTAAATGTTTCAAATTTATTTAATTTTACATATTCTAATAAATAGCCAAAGAAGAAAAAATTCACCATTATATATCATGCTAAAACATATAAAACAATTGCGATTGCTGTTTGCTGATATGTAAATATAAATTGGCGATATTGGTTCGAAATGACATCGTTTCGATATAAAAAATGTGGATCAGAAAAGAAAATACACAAACTAATAATCATAATTAATACAAAAATATTATTAATAATAATTAAATTTAAAAATTTAGGAGCATAAGTTTTTAAATTATTTTGCAATCGTATTTGGTTAGTATTATCAAATGTTTTATTAATAAATTTCTTATTATTAATCAAGTTATCACATCCTTATCGCTTTAATATTTAAATATTATTATAGACAAAAAACATTGCATAATTGAAATTTATGCTCAAAATAATTTTTATTTTTAATATTTAAGATTGAACTTGTGTGTTATATTTAATATTGACAAATATGAAAATGTTTGTTTTCTAAAATTATTTGTTTTAAAACTTATGATATTAATTAAGTCAAAAGAAAGGAGTTTTTATGGATCAAGTATATAACATTGTCATGGCTTATATTCTTACATTAATCATATCTCCTTTATATTCGTATTTAATTGGTAGTTTAAATGCAAGCATCATTTTATCCTTAGTTTTTAAAAAACAAGATGTTCGTCTTTTTGCTTCTAAAAATGCAGGCATGACTAATATGATACGTGTTCACGGAAAAAAATTAGGTATATTAACGCTTTTTTTAGATATTATTAAACCAATCACCACAGTTAGTTTAACTTACATTATTTACAAATATGCTTTAGACGCTCCATTTGATTTAAGTAATGGTTTTAATCAAGCCATTTTAGTTTATTTTGGTGGTATTTTCACCATTATTGGTCATTGTTACCCAATCTTCTTTAAATTTCAAGGCGGGAAGGGAGTTGCATGTTATGGCGGTTTTTTAATTACAGTTGATCCAATAGTAGCGCTAATTGGAATCATTACACTATTGGTCATTTTACTAATTACTAAATATATGTCTTTATCTGCTATGATTACTGCAACTTTCACTTGTTTTTTAGTATTAGTTCCAGGAATTAATTACATACCATACTATAATCAAAATTTCACTACGTATTTTTTTGATTTAAATTATGTTATAAAAGGAATCTGATATGTTTGGTTTTTCTTATTAGTTAGTGCAAGTTTATTAATATATCGACACAAAACAAATATTTTAAGCATCGCTACAAAACAAGAAAGAAAAACAATTTTATTTCACACTAAGTCAAAAGATAATCTTAGTGATGTTAATAAATAATATTATTAATTAACAATGAATCTATTTTAAACATTGTTATTTTGCAATGGAGTTTTTTTTATGGACAAAAAGAATATTTTTACAATTCTAGAGAAGTTAGTTAAAAAATACAAGTATTTGCACAAACCAATTCTAGATGACTATATTAGTACTTTTACAAGAACCTTTTTAGAATATATATTGACAATCGAAATGAAATATCATTTAAATTATGAACGTTTTGAACGTTATTTAAGTAGTAATTCTAAAAACTATCGTAATGGTTTTTCTAAAAAACAACTGTATATGAACGATTTAAAAGTTTCAATTAATATTCCACGTGATCGTAATGGAGCTTTTGAATCAAAAATTATTTCAAAATACCAAGTTGATATTTCACATTTTGAATATCGCTTATTATCGTTGGGAATCAATAATTTACAATTAAATGAATATGAAAAAATATTAGAAGATATTTATAATATTTATAATGACAAAATGGTTGATAAACTATGTAATGAAATTGCTTTAAAAATTTTTGATTATTTAGAGAATAAAAAACAAGAACTAAATAATATAGATGTTGATCAAATTTTTATCGATGGTTTTAATTTATCATCAAATAAATGATTAACAATTGTAATTACTAAAGATTTAGAAAAGCGTTTAACAATTTTAGGTTTTTGAGTTATTAATAATTTAGAAGACAAATGATGAAATGATATTATTATAAAAATTTCAAAATATCATCCACAAGTAATTAATTATAAAGTTATTTTTAAAGATAATGATGAACTGTTAATGTCTTTAAAAAATCATACTTCTAATAATTTTGAGCGTGCTCGTTTTGTTTTTATTAATAAATCTATCTTAAACCAAAAAAACTAGTATTGCATAATCAATTCCTCTTAAATCACGACATTTCTTTATCCAATTATTTTATATTATATATATATATATAGAAAGTCAACTAAAAAATAAGTTTAATGTTTTTTGATAACATTAAACTTATTTTTATATTATTTTTTTTGAATTTATTTTTAATTATTAAAAATATTTTTAAATATGAACACTTGTAATTAAGTATGAAAAAGGACCAATAGTTTTTATATTTGATTATTTTTTACCATAGACACTATAAATAGCAGCGTTTAAATCTCCACCAAATTCAAAACGTGATGAATTATAGACGTTGTCTCCAGAAACATATGATTCTTTACGTTCATTTTTTGATTTGTCATATATTTTTGAACTAGCATAAACTTGTGGACCCTTGCTAAGATTGTCTTGAGAATAAAACAAAACAATTCCAGGAGTTTGTGATGTACTTAATTCAATTAGTTTTTCTTTTGATAAATATTTTTTTAAAAAATTTAAAATTTCACGAAATTTAACAGCTTGAGTGTCGTTACGAAAATAAACTTCTCTTCCACTTTTATCTTTATACTTTTTATTAACATCAAAAGAATAATCTGTTGGTGCATTGTTAGCTCAATTTTGATCTTCTTTAATAGTGCTTGATTTATTTTGTTTAATTAAATTTTCATAATCTTGTTTTTGTTTAAAAACATTAGTATCTATTAAATCAATAAAACTATAGAATTCTGGGACATGCTCATAAACACCTTTATCTGCATAATTTTTGACATTATTAATTGATTTTGCTAATGATCCATTAAACTTCAAATTATCATTATATTTTAAATCACTAATCGAATTAATATGGTTAGTTTGATTATCATAAACAAAATTTAAATTATCAAGGTTTGATTGTGTTCCAATATATAAAACATAATTTCCATTATTAATTTTATTAGTACCTAAAAGCATTTCTTTAATTCCAATATTAGCATCTGATAAATTAGTGTAATTTAAACTTTGATTAAGATCGCTTGATTTTATTAATTTTGCATAAATATTTGTTTTGGCTGATAATGACAAAAAAGTACTAGTTGCGACAATCGCACTAATCCCAATTAATGAAGAAATGGAAATCAAAATTGCTTTTTTCATTTAATATACCTCTTAAATTAATATTATAAATAAATTCACGCATCAAGAATTCACTATTAAAAAATTATATAATATTATAATAATTAAAGATGGTGATTTCTAAATATTATGGAAGTTAAAGTAAAAAAACGTGTCGTTGTTGGCTTGTCTGGTGGCGTAGATTCTTCTGTTAGTGCTTTATTATTAAAACAAGAAGGCTATGAAGTGATTGGTTTATTTATGAGTAATTGGGATACAATTGCAAATTTTGAAAATAACCATGAATTTAATAAAACGCATCAGGGTTGTGAGAGTGAATTAGATTACCAAGATGCTCAGGCTGTTGCTCAAAAGATCGGTATTCCATTATATCGTGTTGAATTTATTAAAGAATATTGAGATAACGTTTTTGAATATTTTTTAAGCGAATATCAAAAGAACCGTACACCTAATCCTGATATTTTATGTAATCAATTTATTAAATTTGATTCTTTTTTAAATTATGCTAAAAATGAATTACAAGCTGACTATATTGCAATGGGTCATTATGCAAGAGTTAAGCATGATAGGAATTCATCTTTTTTACTAAAAGCTATTGATACAAATAAGGATCAAACTTATTTTTTGTGTAATTTAAACCAAAATCAACTACAGAATGTGCTATTTCCTATTGGGCATTTAACTAAACTACAGGTGCGCGCTATTGCTAAAAAACACGGTTTGATTACAGCTAATAAGAAAGATTCAACAGGGATTTGTTTTATTGGTGAACGTAATTTTAAAACTTTTTTACAAAATTATATTCCTAACCAACCAGGGCAAATTATAAATATTGTCAATAATCAAATTATTGGTCATCACATAGGAACAATGTATTATACAATTGGACAACGAAAAGGATTGAATCTAGGTGGCATGAATGAGAGAATGTTTGTTTGTGATAAAGATATCGATAAAAAAATAATCTACGTTGCCCCTTCAAGTTTTGAAAAACAATATTTAATTTCAACTCAAGCATTAATTGAAAATATAAATTTTATTGAACCATATAATCCTCAAATTCCAATTATGGTACGCTTTCGTCACCGACAAGATTTAATAATAGTAAACGATTTTTTACCTATTAAAAATACCAAAAATGTACTAATAAATTATGAGTCAGCTAGAGCTATTACTCCTGGGCAATATGCTGTCTTTTACCAAAATGATCATTGCATTGGCGGTGGAATTGTTTCTAAAACTAATATTGGACATCAAAAAGTGGATTTTTTGGTATATAAATCATAGAATATTTTCTTTTTACTATTAGAATGAAATTGTTTGTCAAAACAAAACAATATTATTTTAAAAATGAGGATAATAAAAATATGTTTAAGAAAAATAAAAAATCTTTAGCAGTATTGCTAGTAGGTTTTTCAACTGTTTTAATTTCAAGTATTGCGGTTGCTGCTACAGCATGTGCAAGAAAAGACAATAACGTTAAAGAGGAAGGAAAAACAACACTTAATAAGTTAATTTCAAAAAAAGCTCCAAAACAAAATGCAACTAAATCTAGTGAAAAATATATCTTAAGTGATGTTAATAATAGTAGTACTTTTTTTAATTCACTAACAGACCCTTATAAATGTTTTGAAATGTTTGCAAAATCATTGGATGATGCAGGTTATGAAATTACATCACATGAACCTTTGAAAAATAGCGGTAAAACAAGTTTTTTAGAGTATGCTAAAGAACTTAATAATAAGATTCGTGAATATGTTAGAAAAGTTGCTAATTTACCAACTATTAAAAAATTAAGAGATAATAATACAACTGTTTTATGAGCAGAATCAACACAAAGTTTTAATAGTTCAGATGCATCTAAATTAGATTTATACTATTTCCAACAAGAAGATTTATTCCCTTTATTGTATTCTCAACCAGATGATTCAAATGTTCCTGGGTTTGGTTTACGTTTTCCTAAACCAAAAAACCATAATGCTGATAAATATTTTGATAACTGACATGGAATTGGTGCATCCCAAAATAATGCGTCAAAAAATGACTATGATTGAATGCTATCAATGCAAGAAGCTTTTTATGGAACTGCGGATTATGTTTTCTTTAATTATGACTCAAACAATCTTGAAGGCAATGAAGATGGAGAGATTTTAAAATCAAATTTTGAAAAATTCTTTAAGAAAACTGTTAATAATAACAAAATTATTCCAGTTGATTTTCAATTTGAATATTCTTCAATTTGAGGACCTATTGGAACAGTACGTTTAGTTCAATCACTTGCTAAAAGATTAGGGGTAAGTGATAGTGAATTAGCAAGTGTTAGTCTTGATTGAACTATGCCATCAAAACAAACATTAAAACACATTCGTCCAAAAAATTCTAATAATGATCATAATAAATTTGCAATCTGATTCTATAATTTATGAGATCATTGTGTGGCATTGGGAATTCAACCAGACTATGCTTCATTCGTTAAAGAAAAAGGAAAAACATATACTAAACTACCAGGATTTTTAGCTGATTTAGTAGATGAAACAAAAACCGAATTTAAAGATTTCACTGGTGATGCTCAAAAAGTTGCTGGTATGAATTTATATGCTCTAGGTGGTAATAATTCTCATATTAACCGTTCTTTTAAAACTCTTATTGGCAATGCAATACCAAAAGCTGTTCCTACTGAACGTGGCGATTCAAATAATTCTCGTTCACCAATTAATTTTGAATTCTATGATCGTACAACAAACACATTACAATCTGTTGAAGGAACAAAAGTTGTAAGTTGAACAGATCCAGCAAAAGAAAATAAACAAAAATAATTAAGTTTTTATGTTTAAGAATAAATCATTAAAATTTAAAAATAAATTAGATTGAAAAACACATTTTAGAAGACTTATAACATCTTTATTAATTATTTTAAGTATTTGTGTGCTAATTTGTTATTGTCTTTTTGATGGTAAAGAGTTAATGAATTTTAAAAATGTAGGTGAAGAAATAAATGAGGGAAGTGCCCCTTATATTCGTAAATTTATTGCTACTCCAATTGCAATCTTTTTATCTGCATTAACATTATCATTTAGTGGGTACAGTATGCAAGTAGTTTCACGAAACCCACTAGCTTCACCAACTACATTAGGATATTTACCTGCTGCAATTTTAGGTTTAGCAATTAGTAAACTTGCTATTAATCAAATTTTATATCTTCCTTTCATTATTGGTATTGTTTTTGCTTCATGTCTAATTGTAATTAATTTTTTCTTGGTAAAAGGAAATGCTTTAGAAGCAAGTTTTAAACCAATTTTAGTAGGATTTGCAATTGGAGGTATCATTACTGGTATTAATGTATTATTAGAAGACCTTGTAAAAGATATAAACATTAAAATTACAGGTTTCGTTGAACCTCCAATTAATTTTCAATGACAACAATTATGTATAGGTGGTCCATTAATTATTATTTCTAGTTTAGCAAATTTATTTATGGCGCCATATTATACAATCATTGCTAAAGATTATTTATTAGCAAAATCTTTAGGAATTAAAGTTAATTTGATTTTTTGGCTAACAGCTTTTTTTGCAGTTGTCGCAACTGTTTCAAGCATCATTTTAATTGGTGTTTTAACACTATTAGGAATGATAGCGCCTCATGTGGCACGAATATTAAATCCAAAAGGCAATAGTTTTAAACAATTATTATTATCATTTGTAATTTCATTATTACTATTAACATCTTCACGTTGATTAATTAATGTCTATAATCAATTTGATATTAATTTATTCTCAGCTATCGCTGCCTTACCAGTCTTTGCATATATTTTTGTTTCAAAACAATACCGTCGGAATGTGGAATAACTATGAAAATGAAATTTAATTTTTTTAAAGATAAACAAAATATATTAGGACAAAATCACATCTTAGAATTAAATAAAAAAAGTTTTATTCCATATCATTTTAAACCTTTGTTAATAACAATAACTTTATTGATTGCTTTATTAGTTGTCATTATTTTAATTTTTTCTGTTACCTTATCTAATAAGTTAGGCTTTAGTGAATTAGCTAAAGTAATATGACAAGAGGTTGTACAATTAATTATTACTGGAGTTGCTTTAGGTGTAAGTAGTTATGTCTTACAACGCATCACACGAAACCGTTTTGCAGATGTATCAATAATGGGTATTGGGACAATTAATTTAATTTTATTATGTGCTTTAAGTATCCCAGTTGATTTTACAAGTGCAAACGAATTAAGCATCTTACAAAAAAAAGAACCATGAATTTTTATGTTTATGAGTTGTTGTTTAATGGTTTTATATTTCTTTGTTTCACGTCAAAAAGAAAACTTTAATTACAAAAAACTAATTTTAATAGGAGTTATTTTAACATTCTTTTTAATTGCAATTGCACAAAGTATTCGTGGTTGATTAAATTATCACGCTAATGATTATGTTGTTGGCCATATTGTAGGGTCAGTACAAAAAGCACCATTAAATTCATTAATTATTGCAGCAAGCTTTGTTATTTTAGGCTTATTATGATTATTATTTAACTCATACAAACTAAACATTATTGCTACTAACCAACAAGTAGCAAAACAATTAGGAATAAAAATTAATTTTCAAATTTTTATTGCTCTAATCTTTGTTGGTATAATGGTAGGTGCAAGTTATTCAATATCAGGCGATTTTGTTTATGTTGGTCTATTAGCGGGTAATGCAGCAATGCGTCGTCGTAATAATAGTTTCACATACGGAATATTAAATTCAGGGATATATGGAATGTTGTCAACATTAATTACTTATTGAATTGGTATTTCATTGATCGGAATGAGTGTTTATCACGTTGGTGCTATATTACCATTATTAATTGGTCCATATTTCATTTATAAAGTTTTAAGATCATAACATACAAAAGAATATCAAATTATAAAAACCCTTAGATTATTTATGAATCCAAGGGTTTTTATAATGCAACTGTATCTTAATTATTTGTTTTATTATGAAGGACGACGTTTTATAGTATTAAAATGATTGATTTGTTGGGTTAATTTATTTTTAGTTGAAATTGAATTTTCTAAACTATGATTAATAACATTTTCAAAATTGTTTAAAAATTCATCATAAATGTTAATAAATTGCTTTAAATGAAATATCAATAACGCAATTGATGAGTTTTTAATATATGAATAAGTAAGATTTTGATCAAAATTAAAATGCGATTCTATTAATAATAATTTATCAAAAAAATCTTTTTGTAAAAGATCAAGTTTGTTATGAGCTAAGAAAATGACTATGTTCTTTGCCATATTAACTAAATAGATTGCTTCATCAATAACACAGGCTTCAATTAAATTATTACATTTTGTTTCATAAACGCTTAAATCAGTGTTGTCATCATTACCCAGTTTATTACTAATTTCAATGTATAAGCTAGTTAATTTTTTAGCATTCTTTTTAGCAATATTAGGATCTTCAGCAATTTCTTTTAGAAAAAAAATAAAATCAGTTGTGTCATTAAAAGACAAATTAATTTCACGTTTTGTTTCATCAACTTGTAAAAAACGTAAAATCGATTTTTTACTATCAAACTTCTTAAATTTCATAATCGAACTCCTAGTATTAAACTAATTTAATTAATTATAAACAATATAAATACGTTTAAACTTATAATTTTATTATAAGACATTAATAAAAGGTTTTTTATGGCAATTGTTTCTGTTAAATTAACATCTAATCAAGTTGATAAATTCTTAATTCTATGAAGACCATATTTAGTACATAACAACCATAATTATGCTAAATATACTTTTAAGATCGATGGTACTTTAATCATTATTTACAATACTAACACATGTACTATTAATACTCATGATATAAATTCTTTTTTAAACACATTTTTAAAAAGAGAAGATTTAACACTATTTAATGTTATAAAACAACAAGTAAACACTAAAAAAAATAATCAAAATGTTATGAGCCAATCAGAAATTTATGAAAGTGCCCAAGTAATTATTGGGTCTGATGAGGTTGGAGTTGGCGATTTATTTGGAGGTATAGTTGTTTGCGCAGTGAGTTTAAAAAAAAGTGATTTTAAAAAAATAAATCATTTAAAAATCATAGATTCTAAAAAACTCAATGATCAGCAAATGCAAGAAATATATCAACAAATTAAAAATCAGATTTCATATACAATTGTTTCTTATAATCCTAAAGAATACAATGAATTAATTAAAGAATACAATAATGCACATATTCTTAAAACTATTTTACATTATAAAGCATTACAAAGTGAAATTCACAAACACGCTAAATATTCAATTTTTAGTGTTGTTGATGCTTTTAGTTCATTAAAAAACTGAAATCAATATTTACAAAAAGTCAATTTTCAACCTTATGAACCAAACTTATTAATCCCTAAAGCCGAATCAATTTATACAAGTGTAGCTTTGGCGTCAATAATTGCACGTGTTATGTTTTTAAAAATGATTGCAATTATTGAAGAACAATTTAACGTTAAAATCCCTTTAGGAAGTTCAAATCCTTTAGTTAATGACGTCGCTAGTAATATTTTTCAAAAATTTGGCTTAAAAATTTTAAAACAAGTCGCTAAAGAACACTTTAGTAACTTTCAACAAATTATTAAAAATAAATAAAATCTTCTTTTTTAACTAATTCTTTTAATGTAAGAAAGGAATTAACAATATGCAAAAAATATCTGATAATTTTAAAAAAGTTTATTATGGTGGTTTTTCAACTTCTAACTCTTTTAATTATATGTGAAATAACGCTTTAATTTCATCAATGTTAGGTCAAACAGTTGTTGGATTCTTAGGAATGATTTCACAATTAGTTTATGCTTTTAACCCTCAACCAAAATTTAATCCTCAACAAATGATCGATAACTCTGTTGCTCGCACTTATGCAAGAATCGCACAAAAACCAATGAATTCAACGATTAGTTTAGGAACTCCATTTTTTTAATATGCAAAGAATAATTAATAAAAACATGATTTATGGAGGATTTATGCCACTTGTTTCTGGAGCATTAGGTTTTTGAAATAAAATGTTATTTGGGACAATTATTGCTAATACAGCTATTTCTATGATTGGAGGCATAACAAATATTGTAATGGCTAGTTCATCACAACAAAACCACGATTCACAAGATATAGATTCGATTAAAATAGAAACAATTAATGTAGATATGAGTACAATAGTTGGTCCTAAAATTCCCTATATGAATATTTATTATTTAAATTCTTAAGTTATAATTTAAACTAGTAGAGAAGATAATTTAAATCAATAGGTGTGTGCATGGTAAATGATAATAAAATTAATGAGTATAAAAATAAAAAATTTGTTGCTAACAAAGTTGGTTACGATCCTGAAAGTGTAGATGTTTTTTTAGACGAATTAATTAATGAATTAGAAATATTTATAAATGAACATAAAAATGCAATATTAAAAATAGAAGAACTTAATGAATATAAAAGAAAATTCGAAAAAGAACGTGAATACACAACATCACTAAATAATTTTATTGATCTTTTAGAAAAAGTTGTTGATAAAAAAAGCACTTTTGATGTTTGAGAGAAACGTCCGGTAAGGAAATAAAAAAATGAAATTAGTTCATGGAATTGATATTATTGAATGAAATCGTGAAGAATTAAATAATCCTTTATTTGCAAAACGGATTTTAATTGATAATGAATTAGAATACTATTTTCAACTTAATTCATCAAGGGAAAAAAAACGCTATCTTGCAAGCGTTTTTGCCTGTAAAGAAGCAGTTATGAAAGCTTTAAAATTAAAATATGGATATGGAGATATTTTAATTCTGAAAACAGAGAATCAACGCCAAGTTTATCTTAATAAAATTTTAATAAAAGAGTTAGAACTTTCAATTTCATACACAGAAACTTATATAGTTGCTTCTGTAGTAGGTTTAATTAATAATATGAACTAAATAAACTAAGTTTTAGTAAGTAATTATAATTATTTATAATCTCGTTTTAAATAAATTAAAGCCAAGCTTATAATTAAAATCGTACTAAATAAAATTCAAATTAATCAATAATAATTTTTATTAACAATTTGATCAGCACGTTTAATAGAATAAACTTCTTTTGCTGTTTGAAAAACATAATTATTACTTTTTTGTAGTTGGTAACGATATAAAGTTTTGTTATTAACTACTTTTTTCATTAATTCATAACTTCCGATCCCTCCAATATCATATGCATAATTATTTACATTGATTCTTATTGATGATGGACGATAAAAATTAATAACTTTATCGTTTTTTAATAATTTTTCTAAAAGATTTCAACGTTCCTCATTAAAATATAAATAATACATTAAAGCTACAATGAAATAAATTTTCTTTTCAACAAGATTTTTAACATAATAATTATTTATCTTTTTAGCAAATAGAATACTATTTTCATCAACGATTGTTGAAAAATTTATTGATGATTGTTCATTAATTACATCATTATAAGCAGCAATAATTTGTGGTTTATCAATATCTTCATCAAAATTATCATAAAATTTTTTGGCAAAGTTATTGAAAACTTTTGATTCTAATGTATTTCTAATAACCGCTCATTTTAATTCACCAACAAAATCATTAGTTGATGAAAATAAATTATCATCCTCAATGAAATTAACGTCAAAACGATCTACATTTTCTCTCGCATAAATTAAATTACGATTCTCAAGTGTTGGGAATTGACTCGTATTTTTTAAAGCTCCAGGAACAAAATATTCTTTTTGATTTTCATTAATATTAAATTGTGGTAAATGAGGGTGTTTGTTAAGTTTATAATCATATAATTTTGATTCTAAACCATGATAATTAAAATATTTATCTAAATAATTTTCGTGTTGGCTATTAACACTAAAAGGATCAATATCATTCTTATAAAAACTATTATTTAATTGATAAGGAACAGATAATCAAGATAAGACATGAAAAAATGTCGATGCATTTTTTGCGTGATCAAACGCTGCTTTCAAAAAATAATTCTGACGTTTACTAAATTGTGGATTATTTAAATTTTTAGTGATTAAATAAACTTCATCATTATGATTATTTAAATAAAATTTTTCTACATCTAAAATTGTATTACTATCATATTTATCATAATTAATATTCATGTATTTGGCTACTTGATTAATTTTAGATGTTGAATACAAATTAGCAACACCACCAGCAATTAAAAAGGGGAGAAAACAACTAATTGGAATAATCATAGCAACTTTATTTGATCAACGACTAACAATTAAAGCAGCAATACTAGCAAAAATTAATCCACAGAAATAATAATTAAAAAAAGCATATAAATAAAAAAAATTAATACCGTTATTATTCTTTATATTTACTACGTAAAAAATAATATTTAAAATATAAGTTATTAAACTTCAAAAAACAATATTTAATATTAAAAAACCTATTTTAGTAAAAATAATGTTTTTACGTGAAATACTTTTTGAAAAGACTAAAATTTCAATTCCTTCATCCTTTAAATCTTTGAATAAATTAATAGCTTTAATAGTGCTAAAGACTATTGTTAAAAGTGAATTAATACTAGTAAAAAGATAAAATAAAATCAAATACGTTTTTATATTATTAGCAATATTTAAACCATTTATAATTATAAAAATTAAATTAACAAATAAGCTAATTCCTACTAAAAAATATGTGCTTTTTTTAAAAAGTACCAAACGTGATAAAAAGGTTGTATATAATCAACTTGATTGAAAATAATTTTTTAACATTGCTAATGTCTATCTTTCTAAAAATTTAATAATAATTATAAGAATAATACTAATTAATAAAAATAATCTTTTTTACTTTATATTACTAAAAAAAGGTTTTAATTAAATATTTGGAATAAAAAACCACCATTTTATTGTTGGTGATTTTTATTATTATTTAATTAAAACTTGATCTAGTTCACGATCATAATTTATTTTATAATTTATGTTTTTTGTAATACGATTTTCAATTATTAATTTAGCAATTTCATTTTCTAAATGTTTTGTAATTCATCGTTTTAAAGGTCTTGCACCATAATTTGAACTATAAGCATCTTCAACCATGATTTGAGCAATATTATCATCAAATTTAATTAAATAATCTTGCTTAGCTAATAAATTTTCTAATTCTTTAAGTAAATTTTCACTAATCTTAAAAATGATTTCTTTTGATAACTTATTAAAGAAAATAATTTCAGAAATTCGGTTTAAAATTTCTGGTTTAAAAGTTTTTTGTAAATCAAGTAATGCCTTTGTTTTATTATTTTCTAATAAAGCTTCTGCTCCAATATTAGATGTCATAATAATAATTGTATTTTTAAAATTAATTTCATTATTTTTAGCATCCTTAATGCTACCTTCATCCAATATTTGTAATAAAATATTTAAAATATCAGGGTGTGCTTTTTCAATTTCATCAAATAACAAAATACTATATGGTTTGCGTTTAACAGATTCAGTTAAAACTCCAGCATTTTCATAACCAATATAACCAGGTGGAGCACCAACTAATTTTGATACACTATGTTTTTCCATATATTCACTCATATCAAATCTAATTAAGGCTTTTTCTGAATTGAACAATTCTTTTGCTAAAGTTTTTGCTAATTCTGTTTTACCAACGCCCGTAGGTCCTAAAAATAAGAAAGAACCAATAGGTTGGTTAGGATTATTAATACCTGCACGACCACGGATTACCGCATCACTTATTAATTTAACTGCTTCATCTTGACCAATAACATGACTAGCAATTCGTTTGTTTAGCGATAATAATTTTGTTTTTTCATCAGCTAATAAATGACTTAATGGAATTCCTGTAGCACGCGCAATAACATCAGCAATTTCATTCTCTGTTAATGAATCAATAATCATTTTTTGATTATTATTCTCATTAATTTTATTTTCTAATTCTTCTCGTTGTTTTTCAAATAATGGTAAATCTGAATAAAGTAATCTTGATGCTTTTTCATACTTACCCTCAGATTGATAAAATTCAATATCATGTTGAATTCTTTCAATTTTTTGCCGTAAATTTTTTAAATCGTCAATTTGCTTTTTTTCTTTTAAATACAAATCATTTAAATTGTTAAATTCATCTTGTTTTAATTTAATTTTTTCTAAAATTTCATTTAAACGTTTAATTGATTTTTCATCTTTTTCTGGTTCTAAAGCGGCTTTTTCAGTTTGTAAATGTTGTAAATCACGTTTAATTTCATCTAAATATATTGGTTGTGAATTAATTTGCGTTTTAATCTTTGCAGCTGCTTCATCAATTAAATCAATAGCTTTATCTGGCAAATTACGATCATTTATGTAACGTTCAGATAAATTAACAGCAGCAATTAATGCATTATCATGGATTTTAATCCCATGAAAGGCTTCTCACCGTGGCTTTAAACCTCGCATTATTGTTAAAGTTTCTTGAATTGTTGGTTTGTTAACTAAAACTTTTGTAAAACGACGTTCTAATGCCTGATCTTTTTCAATATATAAACGATACTCATCAAGCGTTGTGGCGCCAATTAATTTGATTTCTCCTCTTGCCATCATTGGTTTTAAAATATTAGCAACATCCATATTTGAACCTTGATTTCTACCCATACCAACAATTTGATGTATTTCATCAATAAACAAAATGATATTACCATCTGCTTTTTTGACTTCTTTTAAAATACTATTAATTCGTTCCTCAAACTGGCCTTGATAACTAGCGCCTGCAATTAAAGATGATAATGATAATTCAATAATTGTTTTATCTAATAAGTTTGAAGGAACATCTTTATGAACAATTCGGTATGCAAGCCCCTCAACAATTGCTGTTTTACCAACACCAGGTTCACCAATTAAGACAGGATTATTCTTAGTTTTACGACTTAGAATTTCAATGGTTCTTCTAATTTCTTCATCTCGACCGATAATGGGGTCAATTTTATTATCTAAAATTTCCTTATTTAAATTACGTCCAAATTCCTCTAATGGATTGTTTGAGTTTTTTTGTTTAAATTCCATAAATAATCACCTACTTTACTATTATAATTTTAGCACTTTAAAATAATGAGTGCTAAAAAATAAATGTATTGTCATAAAAAAACAGGTTTAAACAAACCTGTTATATTTATACTCTTTTAAACAATAAAAACAAACGAATTACATATAATAAAATACGATTGAATGAAGCAAATAGTAAATAACTATTTCAAGTTGTAAAATCACGCATTATTTTTTTATTATCTAAATTATGATGTTCGGCCATTACTTTAATAATGTAAATCGTCGAAGCAAAACCAATACTTAAAGCGAGCAATCAAATAATACTTAATACAATGTCAGCAATAATAACACCTTTGTTAAAACCAGTATGGTTATATCTCGTTGTAAAAAATACAATCAATAAAATAATTGAACCAATTGTAGCAATAGTAAATAAAACGATATTAGCAATGCCTAATTTAAAAGCAATTTTATGATTCATAATAAAACCTAGTATTCCCATTGCGACAATGATTGATAAACTAATTCCAATGGCTATTAACGTCAAATCTAATCCTACAATCGCCGCTACATAACTAATCATAAAACACATTGTAATACCATCCAATAGATACAATATGATTGATCATGCAAATTTAGGAGCTAAAAAATTATTTCTTTGTCTAAAGTAAATGATTAAATGAACAATAAAATAGACAATTGATAAAGTAGCAACAATGATTGTAATTATTTGAAAAATCAAACGAGAGCTAATAATTAAACTAGATGCATCTGAAAAATAAAATAAATATTTTCGCATTAAAAAACCAAAAATAAGAACTAATATACACATAATTGCACATGAAAATGATGCTTTAATCAAACTAGTATTAATAGCTTTTTTTATTTGGTTATCAACAATTGCATCACCTTTTAAAAATTCAAATGACTTTAAATTCAAATTAAACCTCCAGAAAAGATAATAAATATTTCATAAATTTTAACAATTTAATAATATATATTAATCAATTAATTTAATAATATTATTTTTAACTTGATTATAGTTTAAACAAATTTCATAATCATTAATAACGATTGTTTTATAACCACGATCCATTAAGAAGTTAAATTGATCATAAATTTCTAAAAGATTATATGATTTTCATTCATTAAACTCTTCTATAAAAATAAGTAAATCGATTTTTTGTTTTTCTTCATTATATAAAGCAAAAGTGATTTTAAAATTACCAATTTTTAGTTTATATAAAATTTGTTGATTTGTTTTTTTATAATCTTTTAAAATTTTCAATATGGTTTTTTCTAAACTTGTTAAAAATTCTTCATCAAAATGACTACAATCATTAAATTTATTTTGATTAGTCAAATGAACAATTTCATCACAATAACCAATAAAATTTTTAAAAGTCCTAATATTATCATTATTAATATTAACCATCATATCCACAGCTTTAATTGATTTAACAACAATCATTTTATCTGCTGCTCTTGTAATCGCTACATTTAACCGATTAGCACCACCTTTTTTCATTAATGGGCCATAATTATTGCGTAAAATTCCTTCTTTATTTTTGGCATAAGTAACCGATAAAATTACTAAATCAGCTTCATTACCTTGAACATTTTCTAAATTAGTAACAATAATTTCATCATTCTCAAAACGTTGATAAACAACATCATTAAAACTAAAAGTTTGTAGTATTAAATTTTCAATTAATTCACTTTGTTTGATATTAAAAGTTATAACAATAATTTTTTGATAATTGGCTGCTCGTTCTTTTAAAACCTCCATAATTTTTTGTGCTTCAATAACATTTTCATTTTCAAATACACCTTCAACATCTATAATTTCCATTCCTGGATCAAATAACCCATTTTTAGATGCAAAATGTAAATCGCCATTATAAATATTATCGTTACTAAAACTAATTAAATCTTCTCGATTAGAACGATAGTGGTTTTTTAATAAATATTCTGACCATAGAGCAGTTTTAGCACGATCTAATAAACTTTCTGCAACATCTACATCTGCTAATTCAAACTCATCGTTTTCATTTTCTTGACGATTAATAAAAAAATTAGTAGGGCATAATTGTTTATCATCGCCTGCAACAATATTAATATTACAACGATAGATTGAAGGATAAGAACGTTCTAAAAACATCTGAGAAGCCTCATCAAAAATTCCATAATCAAAACTCTTACTTATTAATGGTAACATCTCACTAACAAGTTCTGGTTTACTAATTCAAATAGGGAAAAGAAAACGTAAAATTTCATAATATTTTAAAATAAATTTCACAATATTAGGTTTTTTTGCTAAACGACAAATTTGCGCAATTTCTTTAATTTTTGTTTGGATTTGTTTAGGAGCAGTAACTAATTTAATTTGCAAATAATCTAAATAGTTATTGTAAATAATTTCATCAGCATTTTTAGAAAATTGAATATTTGCATCTCTTAATTTTTTACACAAATTAAAAAAATCAGTATTTTTAATGAATTGATAATTAACTAAAACTTCGTTTTGATATTTAATTAAATTTTTAATAATATCATATTGCTCACTATTTAATTTTAAATCTTTAAGGCCATAAATTAGTTCATATGATAAATTCTTATCAAAAGCATTCTCATATAAATCTTTAACGTATGCTTTATTTAATAAATGTTTGTTATTTAAATAAACAAGATTTTCAACATTGAAAATCTCTATTGGTTGTTTGATTAAATCTTCTAAAAAATCAAAGCTAAATTTTGATAAAAAAGGATTATTAATATATATTTTAAGTAGATCAAGTTGTTTTCATAATTTTTTTTCATCACCTTTAATTTTTTTAAATAAACCTTTTTTAGCAAATGGATTATTTATTAATAAATATTGCTCAAAAGCATAAGTTCATTCATTATTTTTTAAAGTATTTAAATGACTAAAAAACAATGAATGACTTTTTATATTATCTTCAATAAATTTTTTAAAATCATCTTTTAAAAGATGATTAAAGTTTGTATAAAACTCATTAGCTTCAACATATGTATTACTTAACAAGTTAACATCATCATATATAAGACTTGTTAAAGTCATATTATTTTTAACTAAAAAGCTTTCTAACAACTTAATACGTGGTAGAAAAGTTTGTGATTGATATAAAGATTTTGTAATTAAATTAAAATCAATTTGTAATTTATTTAATTGTTCGCTTTCTTCTTTAAATTTTAAAAAATTATTTTTAGAAAATTCATAATTATATTTTTTAAAAAAATCATAACTAGCTTTAACGTGTGTAATAATTTCATCAAAATTATAAGAATAATTCTCAATTAAATTATTAATTTCATTGATCAATAATGGATCGTTAATAAATCTAATTTCTACTTCATTAAAGCGTGATAATCAATACAAATAATTAGCAAAATTATTATCATTATTATCTTTTTCATTATGTAAATTTTTAATTAAAGATGTAATATTAATAATTTCTTTTTTTAATTCATGAATCTTTTTATAATAAATGTTTGAAATAATATTATTTTTATATTTAAAATCATTGAATTGGTTTAAATCTTGATTCTCAATAAAACGCAATCCTCCTATTTTTTCAATTAAATTAAAAATGTTATTATAAAATTGATCATCATTATTTGTATCATAAATAAAAAGTGTGAAAAAATTAATTTTAGCTAATCTTTCATGTAATACATCTAAAGCAGCTTTTTTCTCCGAAATAATTAAAATCGATTTTAAATCATTAATTACGTTTGCAATAATACTTGCAATCACCTCTGATTTTCCCGTTCCAGGCGGTCCATATATTAAAGTATTTTTAACTAATCCTGAATGAATAGCATACTTTTGAAAAATATTTAAAGGACGATTAATTTGGATTAAAGGAAGCTTATTTTCTTCTGCTAAAAAATGATAGTTTTTATAATAAATTTGTTGATTAACATTATTTATTTCTTCGTCAAAAGGATCAATATTATTACTAATAATCACTTCTAAGTCATTTTTAATCGCTCCTCCATGGGGCTCAAAAATTCCTAAAACATATGCATCATATAAAAAAAAGTTATTAAATTGCTCATTAATTTCATTTTGTTTTAAGTTTAGAAATTCATTATTATTAATAATCGATTCTAAATGATCTTTTAGTATTATTTTTTTAATTAGAGTTTGAATTTCTAAATAACTAAAGTTTTTAATTTCATTTAAAGAAACGATATTATTTTTATAATCATTTTTTAAAAAAGATAGTATTTTTTCGTTAACAATAAAATTAGTATTTTTTCGTTTTAAAAAAATTTCACCATTATATTTTTTAATCTCAACATCAACAAAAATTAAGGGCGCGTTAATATTTGAAGCTTTAGGTGTTGTACCAATCAAAAAATTTTTAGCTATATATAAAGGTCAAACACCTGTTTGCGCATAAATATTGCGTGCTTCACGACTTAAATATTCAAATTTTTTTGGTTGACTTTCAATCGCTTTAATCATATCAGCAATTCGTTCATTTTTATAAAATACAAAGTCTTGGCTCCATTCATTAATTTTACGTGTCGATAAAAGATCTTTTAGTTTTAAATTGAAATCCTGTAAATAATCAATCAATTCATCCATTGAATTTATATTTTTAACATCATTTTCAAACGTATTGATGTTAATAAAATTTCTAATTTTAAGAATGTTAATTTTACCTTCAATAAAATCTTGAAGTTCATATTTATTAAAAGTGTGACTAATATCTAAATGGTATTTTGTTAAGCGTGTTCTTATACAACTATCACGCGTATCAGTTGATAGTAAATTTAAGACTTTTTGCTTAATTGCCTTGTTATTTACTCCTTGATCTTGCATAATTTTAATCTTCAACTCCTAATAAATTATTAATATGTTCTAAAACAACGCCTTTGAATTTTGCTTTTTTAATTGCTTGTAAAATTTTGATGTGTAAATTACCATTTAAAACATGAAATTTATCGTTTGTAAACGCCGTTAAAAAAGCCGCAACATCTAAAAATCCCACTACTTTTTTAATACTTTTTAATTTTAATGATTTAGCAAATGCACCTACGTTATCATTGAAACTATTAGTAATTACCATATCGTTATGAGAAATACTATTACGAAAATGTTTTATGATATGTAAAATATCAATAAATTCTAATGGTGTAATATGATTTAATTGAAAATACTCTAAAACACTAATTTGAACATTATTATCAACTGCACGATAAAAGCCAATAGTGGTATTTAATGTTCATGATAATGCTAAATTAATTAAAGGAATTTCTTGTGCGTTAGTATTAAATTGACTTAACAAATTAGAAGAATTAGCATATGTGTAAATATTTTCTGATAATATTTCATAATGTTTTGGTGATTCAAATTTTATAAAAGGATCTGTTCGCATATCTATAACATATTTTGAATTTAAATTATTGATCTTTAAAAAATTTTGAACAACAATAGCATTTAATTGTTGTTCAAATAGCATAATACAATCACCCATTAAATTAGCAATTTTACGATCTAATAAAACAAAATTAATTAAATCCTTACTTTTTAAGCCTTTTACATATTGATTATCTTTTTTTAAAAAAAATGAATAACCATCTATAACTCTTATGACTCCATAATTAAACAAGAAACTAATGAATTTTTCTTTATTTTTAATAGTCAATCCTTTATTCTTTAAATTTTTAATCAAACCAATACTGTGTTTACTTAACGCCATATAAACAAAATCACATCCTATCTTTCATATATTATAAAAACAAAAAACTCATAAAAATGAGTTTTTTTAATAATTGTATACTTTTTATATTTCTATTAAAAATTTTTTATGTTTATTGTTTGATCATGCATCTTTGTTAGGATATTTTCATTGCTAAAGCTATCAAAATTTTTCATGTTATGTACATTGTAAAAATCTTGCTTAGCTAGGGCTTGACTATTACTATATTCATAACAATTTGAATAAATAAAAAATTTATTTTGAAAAACTTCAATTTTAACTTCTTTAAAATATGAACTAAAAAAATAATAAAATATCTTATTTAAATTATTGAAAGGAGTGTTAATAAAAAGAAAGGCTAAATTCTTTGGATTAAAATCTAGTAATGGATGATCAAATTGAGATAAAATACTTTTTTCAAAATGTTCTTTAAAAATTTTTAAAAAAGTATTTTCAATATCCGCACCACAAATAAAACTATAAGTAAACAACTCTTTAGGATCAATATTAATTTCAATAACTTTTTTATTATTATAATTAATATCATTAAAGTAGCTCTCAATCTTTTTAGGATTAATATTTAATGATTGTGCTGTTTGAGTAATGATATCAGCCAGGCAAAAGTGGTTTTTTGTCTCACTAATAACATTACTAATTAAATTATATTCATTATAAGTTCTAAAATTTATTTTAAAATTTTCTAATTCTAATAATAATGAAGTTTTATTATCTTCATTATCAAATTTTAAACCGTTTAAATGAGCTTGTGAAACTATAAATTGCTTAATTTTTAAATTAAATGATTGGCACAAATCAATTATTAATTGATAATCATATTTCAAAATATAATATGAAATCAACTTATAACTATTTTCTTTAGAATAGTGGCTTATTCCAAAATCAACTGCTATATAACTTTTTTGATCTGAACTTAATAATTCACTGTGTTCATTATTAAAATTTGATCTTAATTTCATAAATTCATTATAGTTATAAAAAGTAGTATGTGTAGTTTGAAAGCTACTAAAACCATAACTAACAATAATTTCATCAATAAAATTAATGTGCAATTCTTTTTTAAAACAGTTAATCAATGTATTAAGTTGGATTTTTAAATCTGTTTGCAAAAAATTTGATGATTCATAATTATTTTGATTATTGTTTGGAATAGATTTTATAAAAATTAAATCATTAACTTTAGTTACATCATTATAAATAAATACCAACAATGTAAAATAATAATTATCTAAACGAAGCAAACACATTGGTTTGTTCATAAATAAAATCCTCCATTTTTAATAATTTCTAATTAATCCCCTTAAAATAGATGATCGTGAACGATTGTTATCATTTAGTTCCTGGGTGCTTGGCATAATTTTTTGATTCAATAAACTATATTTATTTACATAATTATTTATTGGGACTTCTTTAGGTAATTTAATTTTTGCATAATTATTAAAGACCTTTTTAACAATTTTATCTTCAAGACTATGAAAGCTAATAATTGCCACAACACCTAAGGGATTTAACATTGAAATAGCATCATTAAATGCTTTTTCTAAAATGTTTAATTCATCATTAACTTCTATTCTAATTGCTTGAAAAAATAAACGTGCAGGATGTTTTTTTTCAAATAAATTTTTTTTAGGAGAATATTTTTTAATGATTTCAACTAATTCCAATGTTGTATTAATCTCTTTAATACTGCGTTCTTTAACAATTGCATCAGCAATAATTTTTGGATATTTTATTTCTCCATATCGTTTAAAAATACTTACTAATTTTGCAAAAGAATAATTATTAACAATATAGTGAGCATTTAAGTTTTGCGATTGATCCATTCGCATATCTAATCAAGCATTCTTATGATAACTAAAACCTCTTTTTGGATCATCAAGTTGTGGACTTGATAAACCTAAATCAAAGATAAAGCCATCAACATAAAAAATTTTATGTGCACTTAATGCTGATTTTAAATTTTTAAAGTTTGTTTTAATAACAATTACATTTGTATTATTTTTAAATAAATTATTTGCAAAATTAATTGCTTCTTGATCTTGATCTAAACAAATTAATTTACCTTTTTTTGAGAGTTTTTTTAAGATGAGTTGTGAATGTCCGCCACGACCAAATGTACAATCAACATAAATGCCATCTGGTTTAATATTTAATAATTCGATTGTTTCGTTTAATAAAACGGTAATGTGTTGATTAAATTCCATTAATTAATAATTTTTCAGCTAAATCACTTAGCGTACTTTCTTGATTTAAGCTAAAATCATCATAAACTTCTTTAGCCCAAATTTCATATCGATCATTTGTTCCAATAATATAAATATCTTTTTTAATTTTAGCTTTATCAATAAGGTTTTGAGGTATTAAAATTCTTGACGCACTATCGATTTCTATTTTATAAGTTTGGCTTAATATTAATCTTGTAAGATTTCGCGCTTCTTTTGAACCAATTGATAAATTATTAATATTGTTAACGAATTGATTAAATTCACTCTCTAATCTCATATCTATATTTCCATCAAAACCAAGTGATAAATAAACAAAAATGGCTTCACCTAATGTTGCTTTAACTTTTGATGGAACTAACATACGATTCTTTGAATCAATTGAATGATTATAAGTACCAATAAACATTTTTATACATTTTTCCCCACTTTTTTACACTTGGCAACTATATTATATATCACGAATTTGAAAAATAGTATACTCTTATACTTTTTTTAATATTTTTTTATAATAAATTCAAAGAGAAGTTGTTATTCAAATAGTCGTATAAAAAATCGTAACAATTTTTACGTAAGCTTTAATTAGAATACTCGCATCAATTTCACTAATAAAATAAATAGATTTTACTAATGTATTAGTAAATAAATCTAAAATTAATTCAAAATGAGGAATAAACATTAATCAAAAAAGTATTTGATATACAAAAAGAATTGGTAAATTCATTACATAACCAAAAACAATACTTAAAAAATTGATTTTTTGGTTTAAATTGATTGAAATGACAAAAGTGATTGATAAAATATACAAATTTATGTAAATATTTTTTAATACCGAATTGTTCTTTATAAAAATATTAATAAGGTAAATGCAAAATAAAGCTAAATATGCAAAAATAAAACTCAAAATCGATAAATTATAAATACTTAAACAACCAATTAACATTCCGCTAATTGCCAATTTATCCATTTTAGAAATTTTTTGAAAAAATTTAATTGATAAAATATGCATAATAAAAACTCGAATAAAACCAATTGAAAATTCAAGTAAATATCCATAAAAAAGTAAAAATAATATTCCTATTAAAAAACTTGTTTTGGGTCATCGTTTAGAAAAAATCTTATTAATGATTAGAATAAATAAATTCAAGTGCAATCCTGAAACAATAAATAAATGAGCGATATTAAGATCATATAAAATTTGCTTAAAATCACTGTTAATCTTTTCATTAAAAATCATTAATTTAATTAACATTAAAGTATTTTCATTTGAGTACTTTTTACTAAGATATATTAAAATTTGTGTTCGTAACGAGTAATTATATTTTTTTTCGATATTTATAACTAAAAAATGTATATTAAAATCTTTGTGAATTAATAAACACAAAATAATACATAAAGCGCTGAATATAATGATTAAAATGATAATTTTATAATTAAATTTAATTGAATATAATCAATAAAATTGACAAATATTAACTATTAAAATTCAAAAATTATTATAATAGAAACAGCAAGCAATACTAGTTGCTAAAATTAAAAAACTAATATAATAATTTTTAGTTTTAAATAAATTATTAATCACAAAACCACCCTTTATTTAAATGTTTGCCAAATAAAGAAATAATTTATATTCAAAATATGAAAGCTTAATCAAACATTATTAAATTGTTTTTTATTATAATTACATATAATGTTTGTGATTAAAAAGGAATAATCTATGCGAACTATTGCTATTGTCGGAAAGCCAAATGTTGGTAAATCATCATTATTTAATCGTATTTTAATGCGCCGTAAATCAATTGTTGACGATCAGCCAGGAGTAACAAGAGATCGAATATATGATATTGGTAATTGATTAACGCGAAGCTTTATGTTGATTGATACAGGAGGTATAATTAGCTCAAAAGATACTTATCAAGATAACATCAATGAACAAGTTTTATTTGCGATTAATGAAGCAAATACAATTATTTTTTTAGTCTCAGCAAAAGATGGAATTAATAATGATGATAAAAAAATAGCTAAAATGTTAAAAGAAAAAGCTAAAGATAAAAAAATTATTTTAGTTATAAATAAGATCGAATCAGAAAAATATTATCTTAATGAAGGAGAACTATATTCGTTTGGGTTTGGTAAATTTTTTAAAATCTCAGCTGAGCATGGAATAGGAATGGGTGATCTTTTAGATGAATTAGTAAAAGATATGCCTATACAAAATAATTTAGAAAAACAAGAACGCTTTAAATTTTGCATTATTGGAAGACCAAATGTTGGTAAAAGTTCACTAACAAATACCATCTTAGGTGAGCAAAGAGTAATTGTTAATGCTGAAGCAGGTTCAACACGTGATTCAATCGATAATGATTTTAATTACTATAATAAAAAATATACAATCATTGACACTGCAGGAATACGACGAAAAGGTAAAATTGTCGAATCTGTTGAAAAATATGCTGTATTACGAACTAAAAAAGCAATTGAAAGATCCCAATTAATTTTATTAGTACTTGATGGATCTGAACCTTTTAAAGAACAAGATGAAGTTGTTGGTGGTTTAGCATATAATGCAAATATTCCAACAATTATTATTGTTAATAAATGAGATAATATTATTAACAAAAATAGTCATACAATGGAAATGGTAAAAAAACAGATTCGTTCACAATTTAAGTATTTATCATGAGCACCAATTGTTTTTGTTAGTGCTTTAGACAATAAAAGAATTCACACTATTTTTGAAGCAATTGAATTCGTTCGTGAGCAAGCAATGCGCAAAATTGCAACTTCATTATTAAATGATGTTGTTATTAAAGCAAATGCTTTTCAAGAACCACCACCATTTAAAGGAGGGAGAATCAGTATTTCATATATCGTTCAAGTACAATCACAAATCCCTACTTTTGTTTTAAAATGTAATAACCCTAAATTTTTGCACTTTAGCTATGCTCGATATATTGAAAATGAAATTCGTAAAGCTTTTGGATTTGATAGTGTACCAATAACATTATATTGACAAGATAAAAATAAAAAATTACGAGGAGAATAAAATAATAATGAGTAAAATTTTAATTGTTGGAAGCGGAGCTTTTGGAAGTGCTTTAACACAAGTTTTAGTTTCAAATTCTCATATTGTTGATGTTTATGGAATTAATGAAAATGAGCTTAATGATTTGAAAGAAAATCAAAAAAATAAGAAATATTTTCAAGATCAAAAATTAGATTTACCAATTAATAATACATATTTGGATATTAATTTAGCACTTCAGAATCAATATGATTTTATCATCATTGCTATTCCTTCATTTGCTATTAAAAACTTTGTTGATAATATCAAAATTTTTAATTTATCAAAAACTATTGTTGTTAATGCAGCGAAAGGATTAAATCTTGAAACAAAATCATCATGGTGTGATTATATTAAACAAAATTTAAAAATTAAAGCCTTAGTTGGATTAGTTGGCCCTTCATTTGCAATTGATGTTTTTTTAAAAAAACCAACAGTCGTTAATTTAGTAGGCAATAATTCAAACGCCCTTATAAAAGTTAAAGAAGAATTTGAAAACAATTGATTTAAATGCATTTTATCTAAACAGTTTGATGCTGCTAATTATATTTCTTGTTTTAAAAATGCATTAGCAATAGGTTGTGGTATCATTTATGGATTACAAAATTCAAATAATTCTTTAGTAGCTTTTTTAACTAAAGGAATCAATGAAATGCAACTAATTTTAGAGAAAATTTTTCACAAAAAAGTCAATCCGTTAGAATACTTTTTTATTGGTGATACAATTTTAACTTGTACTGATCAAAAATCACGTAATTTTAGTTTTGGTTTACTGATTGCACAACAAGGCGTACAAGAAGCTTTAGTAAATAAACAACAGACTATTGAAGGTTTAAATAACATCAAAGTTATTTATGAAATTATTAAAACAAATCAAATTAGTGCTCCACTTTTCCAAAATTTATATAAGGTAATTATTAACGCCTTAAATCCCCAGGATTTATTTGATAAAAGTTTTTGTTAATGAATGATTTATTAAAAAATAAACTAAAACTGATTCCTCACAAACCTGGTTGTTATTTATGAAAAGATCAATTCAATCAAATTATTTACATTGGTAAAGCGAAAGATTTATATAATCGAACCCATTCATATTTTAATGGTTGTAAAGACAATAAAACAAGTAAATTAGTTAATAACATTAATGATCTTGAATATATTGTCGTTAATAATGTTAACGAAGCTTTAATCTTAGAAAACAATTTAATTAAAACACATCTTCCAAAATATAATATTTTATTAAAAGATGGTTCAAACTATCCTTATATCATGATTACAAATGAACAATATCCCCGTTTAAAATATGTTCGTACTTATGATAAAAATAAAGGAATTTATTTTGGGCCATTAGCTGATAGCACAAATAAATATCAATTATTTAATTTATTAAATAGTATTTTTCCATTTAATAAATGTAATCATCAACCATATAAAAAATGTATTTATTATGACCTGCATCAATGTATTAATCAAGTTCAACAATCAACATATCAAGAAGCAATCAGTGAAGTTAAAGAAATCTTTAAAGGTAATTTAGATCACATTTTAATGATTCTACAAACTAAAGAACAACATGCAGTTACTAAATTAGATTTTGAAAATGCTCAAAAATACGCTGAGCAACAAAAAGCACTTACTTCAATTATTAATTCAGGATTAGTGCAATTGGATAATAATGAAAGTTTCGATATCATCGGTTTTTATGAAAAAAATAATTATTTAGTAATTATAATTTTTAATTATGTTAAAGGCAAATTATTAAATAAAAGTGCTGATACATTTGCCATTTATGATTATGAAATTAATGAATTAATAACCTCATTTTTAATGCAATACTATTCACAAAATAAAATTAGTTCAAAAATTATTGTAAGTTTAGATGATGATAATTTATTAGCTTTATCACAACGTTTTCACACAAAATTTATTAATGCGCAAACAAAATTTCATAAACGAATTCTTAAATTAGCTCTTGATAATGCTATTTTGTATTTTGAATCTAATATTAAAAATGTAATTAATAAACAAAATGAATTAGATGATGCTTTAAATCAATTAAAACAAATTTTAAAATTACCAGATTTAAATATCATTGAGTGTTTTGATAATTCTAATATTAATTTAAGTTTACCAATTGCTGGGATGATTGTTTATCAAAACGGTAAATTAAATAACAAATTAAATCGTAAATATAATTTAATGACAACTAAAAATGCTTCTGATTATCATTTTATGATTGAAGTTATCACACGACGTTATCAACGATTAGTTTCACAACATCAAAAATTACCTAATCTAATTGTAGTAGATGGGGGAAAGTTACAAGTTAATGCAGCTTTATATGCGTTAGCACAGTTACAAATAAACATTCCATTAATAGGACTAAAAAAAGATCAAAAACACAAAACTGATGCCATTGTTTTAACTAATGGCGATGAAATTGTTTTAGATCGTAAAAGTATTTTATATAAATTCTTAGCAAATATGCAAAATGATGTTCACAATTATGCAATTTCGTTTTTAAGAGATAAACACACAAAATCTATTTTTAATTCTTTATTAGATAATGTTCAAGGTCTAGGTAAAAAACGTTTTAATGAACTTTTAAAATATTATGATAGCATTAATGATTTAAAAAGTGCATCTGATCAAGAATTATTACAATTTTTACCTAAAAATGTCTTAGTTAATTTACGTGAAAAACTAAATAAAATATAATTCTTTTTTATATATTATTCCTTCAAATCCCTTATCAAAAAGAGTTTTCTTTAGAAGATAAGATAAATATTTTATATAGTTTTTTTCATCATTATTAATAATTAATGATATTAATTTTTATTAAATTATGTTTTAATTTAATAAATAAATTTAAGGAGTTAAATAACATGAAAAAAACATTAGTTTTATACGCACGCGTTTGTCCAACTAAATCGTTTCATACACAACAAATTTTTGAAGCTTTTCAAAAACAAACAAATTTTGTTTGTGTTAATCTTGATGCAAATCAACCTTTTGATGTTTCAAAAGAACAAGAATTTATTTTAGGGTTTGATCGAATTATTTTATTGTTTACGCTAAATTGATATAACATTCCTTGATCATTATCACGTTATTTTATTGAAGTTTGAAGAACTTTTCCTTTTAGTTTAGAAAATAAAGAAGTTTATAAAATCATCACAACTGGAGCTAATCAAGATTTTTATGAAAAAAAAGATCCTAATATTAATAATATTTCAGTTGAAGAATATTTGAATAATGTAAATGGAATGCTGAAAAAATTACGAGCAAGTATTCAAAAAAGTTTTTATTATTATGGTGCAATTAATCAAGATCAAATGCGTTTAAATCAATTTGTTAATGAATTAATAAAATATTTTAAAAATCAAAATTAATGAAAAATTGAGCAAAATCATTATTAATTGATTTTATCAATATTGATTTTCATAATTTTAAATCGATTCAAATTGTTTTAGATGCTTGAACTAATCAGGTATTTGAAGTTGTAGACATAATCAATTTAAGTTGATATATTAGAATTAAAATCAAAAAGGGAAATACTTTAGAGCATTATTTGTTTACTAAATTTTTCAAAGGTTATTTTTATTATGATCAACAATCTCATCACCTAATTAGTCAAAAAATAAATGTCAAAAAACCTTATGTAATTAATGAATGATTTATGGCTAACGTGATTCATAATATCAAAGAATTACAAACGTTAACCATTAACAAAAAAATTAAAATTAAAGATTTTGATTACTTATCTTATTTACCTTTGGTAAAAGATGATCCATTAGCTTCATATTTTAAATCTATTTATGAACAAGAAGTAAAAAGAACTAAACTTGTTTTGGCTCACAATGATTTTTCAATTAATAACATTTTAGCAAATGAATGCGAAAATCTTTTTATATTAATTGATTTTGAATATAGTAGTAGAAATTTAAAAGATTGAGATTTATTTAATTTTTTAAGAGATCTCAATGATGTCCAATTAAATTTTGGAATTTCTTTTATAAAAGAAAAATTAGGATATGACGAATGCTTGATTTATAAATACTTATTTTTAACATCATATTATGGTTTTTTATGAGCTACAAACGCTTTTTTAAAAACTAATGAATTAAAAATTAAGATATATGGCGAAAATGCTTATCAAAAAGCTGATTTTTATTTACAAAAATTAAATAAATAATAAAAAATCCATCTTTGTTATAAACATAATAAGACGGATTTTTTATTGTATTATAATCTTTATCTTTATTAATTAAAAATTAATGTAAATCTTTTGTTTTTAAGTAATCTTCTCAATCAACAAGAGCTGCATTTTGAGAGTATTTATCGCGATCTTGAGGAGCAACTACTTTTCTTGTTTCATCATTAAATCGGCGGTTAGTAATTGCAAAATTGGCAATTTCTTTATGTTCTTTCATTAATGCACCAAACATTTTTGTTGCAGCTTCCCCATGAATTCCAGCTAGAATCAAATTAACATTTAAATCTTTAATTTTAGTTAATGATGTTGTTTCTCAACGAATTCCATCATTTGGAGTAATTGTTCTACAATCTTTTAGTTGTCCATTAATATAATCTGATAAATATAAAGCTAATTGATTTTCTTCATGTCCACTTGTTGATAATTCACCATTTACTAATAAATCAGGTTTTAGTCCTAAAGTAATAGCAATATCTAAAACTTGTGAATTTGTTGCTCAAACTTTTCAGTCAATTTTATGATTTGGATCACGGTGTGATTTAAAAGGATCGCATTCATCTTTAATTGTTTGTTTATCTTCTTTTAAATCTGTAGCTTGGTTAACTAATGTAACTAATTGTTTTGGCACTTTAAATTCTTCTTTAGCTTTTAAAGCATCTAATTCAGATTTTGGCATACCAAATGCTTCTGAAAGGGCATGTAATGAATAATTAACTCCTAAAATACCATAACTTGCATATCATAAACTACTCATTGGCATAACAATTAAATCGTCCTTATTATTTGGATTTTTTAACATGCGTTTTGCAACGAAATCATTAGCATTTTGACTCTTCATTCAGTCTTCAAATTGTTTAATTTTTTCAGGTTGACGTTGATTGTTTTTAAACGCTTCTGGTAAACCTGAATCATAGTATAAGTAAATTACTTTATCAGCTGTCTTTTCAAATGATTCAATTAATTGACCAGGTAGATTTGCTTTTTGAGTATCTGAACCATCTTCAACAATAATATCTCCAAATGAGAAACCATCATCTAATAATTTTTCAGGAGCTACTTTTGATTTAGGTGTTGGAAAACGTGCACCTAATCCAGGAATACCATCATAATCAGGTTTTGAATAAATAATTGGAAAATCAGCAGGTAAATCAATTGATGTTACATTCATAGCTCCTAAATCTTCACGAGGAGCGTTGTTATTTACTAATGTAATAACAGTCTTTTTTTCATCACGTCATTTTTTTCAAAATGGTGATTCTGCATACATTTTAATATCATGTTTAACTTTAGTAATAATATCTTCAATTTGTGCACTAAATGTAGATTTGTATTTTCCATCAGCAATTAATTTTTTAAAATTTGAAGATTTTGTTAAATCAACTGCTTTATCCATTTCCCTTGCTAGTTTTCGTGCTTGTTCAAAAGAACTATTTCATCAATTAACTCACATCTTATCACGATCTGGTTTTTTAGATTTAGCATTCAAAATTTCAGAAATGATTTCATTTCTTAACACTTTTTCTTGCGTTAATGATTTACTTGTTGTTCCTGTACTACTTCCGGGTTGAGTAGTGCCTGTGTTGATTGTTTTTTTAGTTTTCTTATTGTCACTACATGCAGTTGCAACTGCAACAGTTCCAATAGCTATAGTTCCAAATAACAATGAAGAAAACAAAATCTTTTTATTTATTTTTTTCATTTTATATCTACTTTCTTATTTATCCTTAATGAAAATAATTTGGTTTAAAAAATAAACACAGTTAAATTTATACTACTAAAAAAATAAAGAAAGGGTTTTTATATAACAAAAAAATTAAAATAATGTTAGTTGTTCAGTTTCACTTAAATGATCTAAAACGCCTAATACTCGTAAATTTTTTAAATCTGTACGCGAAATTTTTGTACGGTTTTTTAAATCCTCAATTGTTGAAAAAACTTTTTGATTACGAGCTTCAATGATTGAATTAGCTACTGTTTCACCCAATCCTTGAATCGCAATAAATGGTGGAATAATAGCATTGTTTTCTTTATCAATTACATAACTAGTCGCCTGTGATTGTTCAATACTAATATTTGAAATTTTAAAACCTCGAGCTAATAATTCAATCACAATTTCATAAATTGGTTGTAAAGCAAGTTCACGTGAACTAACTTTTTTTTGTGGATCGCGTGATTTAGAACGTTGTTCGATATCATTATAAGTTTTTTCAATAAATTCTTTACCTTGATTAATTACAAATAAATCAAAATTATCAGCACGAACACTAAAAAATGCTGCATAGTATTCTAGTGGATAATAAATTTTATATCAAGCAAATTTTCATGCATGCATAACATAAGCTGTGGCATGTGCTTTGGGAAACATATATTTAATTTTATTAGCTGATTCAATGTATCATTCAGGAACCTTTAATTCTTTTAAAATGATTTGGTGTTCTGGTTTGATCTTTTTACCTTTTCTAACATCTTCCATAATTAAAAAGGCAGTTTTTGGTTCAACATTTTGACGAATTAAATATGTCATAATATCATCACGACAAGCAATAACATCCTTTAGCAATTTACCAGATTTAATTAATGACTGTGCATTTCCTAATCAAACACTTTCTCCATGTGATAATCCTGAAATACGAATTAAGTCAGCAAATGATTTTGGTTTAGTATCCACTAGCATACCACGTACAAAGCGTGTCCCAAATTCAGGAATAGAAATTGCTCCTGTTGTTTCATTTAAAACATCAGACGGTTTAATATTTAGCGCACTAATATCGCTAAATGATTTCATAACTAATGGATCATACATTGGTACATCACGTTCATCAATCCCCGTATAATCTTTTAAAAGTTTTAAAATTGTTGGATTATCATGTCCTAAAATATCAAATTTTAATAAACTATCATGTAGATATTCAAACGCAAAATGAGTTGTTTTTCAAGTTTCATTAATATCATTAGCTGGAAAATTATAAGGTGAAAAATCCCAAATACTATATTCTTTAGGAACAATAATAATACCCCCAGGATGTTGACCAGTTGTGCGTTTAGAATCAATGCACTTTTGTACATATAAACTAACCGTTGAATCACGAATAATTTTATTTTCATTAAAACGGTTTTCAAAATACTTCTTAACCGTATTTTCAGCGCTTGTTTGGGCCATAGTACCAATTGTACCAGCACGATAAGAATGTGTTTCTCCAAAAATACTTTTAATAAAATTATGTGCAACTGCTTGATAAACTCCAGAAAAATTAAGATCAATATCTGGTACTTTATCCCCATCAAAACCTAAAAAAGTTTCAAAAGGAATATTATGTCCATCAGTTAACATTGGAGTTTGACACTGCTCACAACTTTTAGGAGCTAAATCAAATCCATCATCAGCGTTTGTTATAAATTCACATTTTTTACAATTAGGACATAAATAATGTGGTGTAAGCGGATTAACGTCAGTAATATTTAAAAACGTTGCGACAAGTGATGAGCCAACCGAACCACGACTACCAACACCATAACCATCTTGCATTGATTTTTCTACTAATAAATGTGAAATTCAATAAACAACACTAAAACCATGTTTAATAATTGAATTTAATTCTTTTTTAATGCGCTGTTCAACAATTGTTGGTAAATTCTCTCCATATATTTTTTTAGCAGTATGATAAACATAATCGATTGTTTTTTGATCAACACCTTCCATTTTTGGAGCATACAATCCTTCTTTTAAAGGTTGTATGCTATCATCAAATCAACTATTAATATGCACAGGATTCTCATTAATAATCAAGTTAATTAACTTTTCATCTTCTAAATAAGCTAATTCATCAATGGCATTTTTTTGATCAATATATTCTAAATCAGGAACATAAACTTCATTAGCAAATCGGTGTGCTTTACCTTCGAGACCTTTCGTATTAACATAAACATCATAAAATTTTTTATCATCTTTAAAGAAATAATAAGGCGAGCTTGAATAAATAATAATCTTGTTTAATTCTAAAGCTAAATAGATCAATTTTTTAAAAGCTTTTTGTACATTTTCAATTGTAATTTTTTTAGCATATACTAAGTGTTTTTGTGAACCTAATGATGGTAATGTTATAAAATCATAACGTTGAATTAACCTTTTAATTTCTTCATCGTTTTTTGAATATAAAGCATTGATTAAATCTGATTCGTGAATCGAGTTAGAAATAATTAATTTATCTTTTTTATTTGCTAAAAAGCTTGTCGTAATTGTTGGTCTTGTATAATACATATCAGTATGAGATATAGACACTAATTCATACATATCTTTAACATTTGCTTGATTTTTAATATAAAGATTAATTCAATTACCAAAACTACGATTTTTTAACATTGTTGTTTGTAATTTAGTATTAATTTCACTTAAATTAGTAATTCCAAAATCATTAAATAAGTTATTTTTCATAACTTTAAAAACATCAGCTAAAACTATGGCATCATAGTCTGCACGGTGTGCACTTTCATCATTATAATCAACTTCATATTTTTTACAAATCGCTCCTAATCGATGACTTTTAAATGGATTTATTGCTCGTGATATCATTAATGTATCAATACTAGGATTTTTAAGAGGTTCTAATCCTCATTTTAACAGTCTCTGATTAATAAAATTAATATCAAAATTAATTCCATTATGTGCAACTAAAATATGGTCATCAAAAATTTCTTTAATTTTTAATAATGCTGTTTTTTCATCAATTGCATTATTGACCATTTCTTGACTAATATTAGTTAGATTAGTAACATTCTCATTAATTTGTTGAATAGGTTTTATAAAAAATTGAATTTTTTCCCCAATTTGCTTATTATTTTGCATAATCACGGCCCCAAATTCAATAATTTCATCAAAATTAGGAAATAATCCTGTTGTTTCAATATCAAAAATAACATATGTAGCATTATCTAAATTTTGATCCCGAACATTTAAAACTAATGGGATTTGATCTTCTAATTCTTCTGTTTCTAAACCATAAATAATTTTTAAATCTTGTTTTTTAGAAAATTTAGCGATTTCAGGATATGCTTGTACAACATTTCGATCAGTGATGCCAATTGTTTTTAATTGATTCTTAACCGCAAAATCAATTAATTCTTGTGTCGAAATAATTGCATCAAGTGATGACATTTTTGTATGAAAATTTAGTTCTAAACGAGGTGTGATTTGTTTTTTTACATGTTTATCTTCAACGCAACAAATTTTTTTAATTTTACCAATAGGAACTTGACCACTCTTACTCATATTATCTAAAACAATATCAATTTCAGCCTCGATTAATTGATCAATTTTCAGTTCATCTAAAAACTTTTTTAAAGGATGTTCACCGCTTGGTAAATAAGTCATAATTGTTAAGGTTTCACTATCATCAATAACATTTAGTTTATAAGCTATTGCTCCACTTTTTAATATATCAATCTTTTTTAAAAAAACATAAGCTCGAATTTTTGCATTATTCATTAAAGGATTAATATCAATTAATTTTGTAATTGTTTTATTTGAAGGATTTTTATAATAATTAAAATTTGCCTTTTGATTATTATAAAAATTTTGCTGCTGACTAATTTGTTGTTCTAAATGTTTTTGTTGTTGAATATGATGACTAATAATTTCATTTACTGCTTGTTCATGCTTTTCATTTAATAATGAACTAAGATTTTCAAAATGAATTTCAAAATTACTATAAGGTAGTGATCAAACTTTTAAGATGTTTAAAATTTCTAATTTATTTGCTATAAAATGATCATAAATTAATTTATCATTAATTTTTAAAATTAATGAATTTAAATTAGAGTTATATTCTATTTTTGTATTTAAATCATTTATTTGTTCTAAAAAGATTAAATTAGCATTTTGTTCACTAATTTTTTTAAAAAAAGTTAATATTTTTTTATGATCAACTTCTTTTAAATTATTAATATATCAAATTTGTAAATCATGCATTAATGTTGTTTTTGATAATTGATGTAATTCATCAATAATTGTTACATCAACAAATTCGTCAAAATAAACAAATAAATTATTTTTTTTATCTTTAGTTAATTTTTTGATACTAATTTTATTTAATAATTGATCAGAAATTTTTACAATCTTTTTAAATAATGCATTCTTTGTTTCCATTTTAACACCTATATTTATAAAAATAAATGCAGACCAATAATATTAAGTTGCATTTATTACTTGAATAATCTTATTTTTATTATTTTATAATATATAAGTATTTATATAACAAAATTTTGTTTCAAAAATGTTTTAAAATATAAATTACTTGATATTTTATATTTTTAATTTGAATTATCAAACAGAAAAAGAATTAACAACTGCTAAAACTGATGTTGAAAGCAAACAAAAAGTTGCTGATGCTGCTAAAACAAAATTAGATGAAGCAACTAAAGAATCAAAAAACGCAAATCAATTATTAGAAACAGCTAAAACTAATCAAACAACAAAACAAGAAGCAGTTGATAAAGCTAAAGAAGAATTAGAAGAAGCTAAAAAATAAAAATTTATTAATAAATACCACTCAAAAATCTTAAAAACAAGATCTATTGATTGGTTTTTTTATTACTTTAAAACTGATAATTTTTAGAATTTGAATTTTAATAAATAAAAAAAGCCCATTTTTGAGCTATTTTTATTTATATTATTTTTGTGTTTCTATGTTAACATTTAACAATTTAGCATTGGTTAATTTAACATCATAATTTGCATTATTTCCATTCATTTTATCTTTTAAATCAATTTCTAAACCATTAAGATTTGATAATTGATTCTTTGTTAATGATTTATGAACTACTTGTAAACTACTATCTTTTTTAATTTCAATATCAAATGTTAATATAGGTTGATTTTCTTCTAAAGAATTTAAAATATCAAAATTTTGTAATTTAACATTAATTTTCTTATTTTCTTTGTTGAATGTCATAAATAATTTTGCATTTTTAACATATGATTTTAACAACTCTTCTTCATTAAATTTGACTTCTTTACCATTTAAAGTTAGTTTTGAAATTTCATAGTCAACATTTGAATTTAAACCACTTAGTTTACCGCTTAATGTTTTAGATGCTTCATCATATTTTAAATCACTTGCTTGAATTGGATTTGTATCAGCTTTCTTAATAACTTCTAAAACAAAATCTTTTTTATTAGCATCTGCTAATTCAAATTTACTAAATGTAACTTCAATAGTAGCAGTATTATTTTTGACTTCACTAATTTTTACACCACTAGCTACAACATCATCTTTTTTAGAATTTTCTGGTTGATCAGCTTGCTGTTCGCTACCACCATTTTCTTTTGGTTCTTCAGTTTTTGGAGCTGGAGTCGGAGATGGTTGTTGGCTTGGAGCTGAAGCTGCTTCAAAGTTCACTTTTTCTGTTTGATAATTCAAATAATAAAACTTATTAAAAATAATATAGATATATAATTAAATTTTTAATCATAATTTATAATAAAAAATATCTAATAATGTTATCGATAATGCAGAAAAATAAAAAAATAAAAAAAATAGTAAAAATTATATAATTAAAAGTGCAAGTGCTAAATAAAAAGTATTTGCAATCTTTATATGTTTTCGTTAAAATTAAAAATTAATTACTGTAGAAATTATGTAAGATTACCAAATCTTAGTGTTCATATTTTTTACATATATTAAATAAAAACAATAAAATGACATATTTTTTATATTAGGAGAATCATAAATGAAATTATTAAAAAATAAAAAATTCTGAGCTATGACATTAGGTGTTACCTTAGTTGGAGCTGGAATAGTTGCTATAGCAGCTTCATGTTCTAATTCAACTGTTAAATCTAAGTTAAGTAACCAATTTGCTAAATCAACAGACGGTAAAAGTTTTTATGCGGTTTACGAAATTGAAAACTTTAAAGATCTAAGTAATGATGATAAAAAATCATTAAGTAACATTGAATTTAATGCTGCACTTACATCAGCTGAAAACAAAACAGAAAGTACACTTGAAAAAGGTCATTTAGTTGGTGAAAAAATTTACGTTAAATTACCTCGTGAACCAAAACCTAATGAACAATTAACTATTATTAGTAAAAGTGGATTAATCAAGACTTCAGGTTTGTTAATATCTGATAATTTGAATTATCAAACAGAAAAAGTGAACTTTGAAACTACACAACCAGGTAAAGAACAACCAGCAGGTAAAGAACAACCAGCAGGTAAAGAACAACCAGCAGGTAAAGAACAACCAGCAGGTAAAGAACAACCAGCAGGTAAAGAACAACCAGCAGGTAAAGAACAACCAGCAGGTAAAGAACAACCAGCAGGTAAAGAACAACCAGCAGGTAAAGAACAACCAGCAGGTAAAGAACAACCAGCAGGTAAAGAACAACCAGCAGGTAAAGAACAACCAGCAGGTAAAGAACAACCAGCAGGTAAAGAACAACCAGCAGGTAAAGAACAACCAGCAGGTAAAGAACAACCAGCAGGTAAAGAACAACCAGCAGGTAAAGAACAACCAGCAGGTAAAGAACAACCAGCAGGTAAAGAACAACCAGCAGGTAAAGAACAACCAGCAGGTAAAGAAACTACTGGAAAATAATTACTTTAGTTAAAAAATAAAATTTAAATTAAATAGCTCAAAAAATTGAGCTATTTTTTATTATTTTAAAACTGATAATTTTCAGAATTTAAATTTTAGTAAATAAAAAAAGCCCATTTTTTAAACCTGAACAAAAAAATTAGACTCGTAATGAAAAAAATGCGAGTCTTTATTTTTATTATTGAAAGGTTAAATTATTATGGGAAGACAATTAAGAGAAGATGAATGATTATCAATATTTTTTTGATATGAACAATATTTAAATTATGATATTTCTAAAGAATTTTTATCATATAAATATTGTGAAATTAGTAATGGAAGGCAACTAAATAAATATAGTTTAAAACTAATTAAAACCAAATATAAACTCTATAATTTAGGTATGAATATAAATTCGCAAACAGGCAAGGCTACCAAAAAAAGGTAAGGGCTCAAGTCGCACGAGAAAAAGAGATATTGAACAAGAAAAAAAACTTCGTGAAAAAACTTTAAAAGAAATGTCAAGAGAACAACTTGAATATCTCATTACTGATATTTTTTATGAAGAAATTTTAAAACGCAACAATAGCAAAAATTTAGAAGAAGTTGTTGAAAAAAATTAAAAAGAAGCATAATTTTTCAATATCAATAAAGCAAAATTATCACTATTTTAGGAATTAAAAAATTTAAATTGCCAGCCAAAAACAAGGCAATTCAATATGAAAAAGAAATAAAAGAGGCTTTTATTGAATGCAAAGGAAGATTTGGAAGGGAAAGACTTTCACTTTTTCTTAAGAAAAAGTACCAAATCGATATAAACCCAAGGACTCTGGGCAGAATTATGAATAAATTGAGTTTGCTATGCAAAATAAGAAGAAGCAAAAGAAAAAGAGAAATAAAGAATGTTAATGCAACATTCAAAAACTTAGCCAATAGAGATTATGATGGCCAAATGAATGATATATATGCTACCGATGTGACATATATTCCTTCACCCACTGATGTTATAGAAAATCATGTATATTTATCGGTTTTATTGCATCATAAAACAAAGAAAATTGTGAGCTGGAATTTATCAAAATATAATGATACTAATCTTGTTTTAAAACATTTTAAACTAACTAAATTTCCTAAAAAATTCATTGTACATTCAGATCACGGATATCAATATTCCTCTAAGGAATTCCTTGATTACATAGATTCAATTAATGGCACTATTTCTATGTCAAGAGTTGGAAATTCTTTGGATAATAGAGAAGTAGAATATTTCTTTTCTATTCTAAAAACTGAAATATTTTCAAATTTTTTTCAAAAAGCAAAAAGTTTAACATTTAATGAATTGCAAATAGAAATAGACAAATTTATTAATTGGTATAATAATGAAAGATTTATCAAAAAATTTGATTTAAAAACACCCCAACAAATGTGGGATGTTTATATGAACAATAAATCTATTTGAGTCTAAATTTTTTGTCCTAGTTTACAATGGTGGTTTTTTATTTTGTTAGTAATTGCTTTAACAAGCTATAAATATTTATAACAATAGGTTTAATCTATCATTAAAATGAGCCTATTGAAAAAAATACTGGGGTTTTGTTAATTATTTTTTTATTGTAAATAAAAATCAATATTAGTGTCTTTTTACATAGTAAAAAACATTAAATAACAAAATTAATAATTTTATTAACAACAACGATTTGTTTTTTAATTGGTTGATCCCCAATTGCTTGTTTAACTTGTTCACAAGCCAATGCTAAATTAACTAACTCTTCAGCATTTGTATCTCTTAAAACATTGATTGTGTGCTTTAATTTACCATTAATTTGAATTGGAATTGTAATATTTTGAGCAATAATTTTTTGTTCATCATAAATAGGCATTTTTTGTAAACAAACAGATTCTGAATGGTTTAAACTATATAATTCCTCCGCTAAATGTGGTGCATAGCATGAAAGTAAAATTAAAAAATTATCTAATAATTCATAATTAATAACTTTATTTTTGTATAAAACATTAACAAAAACCATCATTTCACTAATCGCAATATTAAATGCTTGTTCATTAATTGCTTTTGTGTAATTTTTAATTAAAGTATGATAGGCAATAATTATTTCTTCATTTAGTTTATTAAGATCTTCGACAACTTCTAGTTCACTTAAATTATGATATAAACGATAAACGCGATCTAATCATTTTCGCATCGCATCTAACCCATCCGGATTTCATGGTAATGAAGCTGTTAATGGTCCCATAAACATTTCATAAATTCTTAATGTATCAGCACCATGCGAAGCAATAATATCATCAGGATTAATAACATTCCCTTTTGATTTAGACATTTTTTCATTATTTTCGCCTAAAATCATTCCCTGATTAATTACTTTATAAAATGGTTCTTTTGTTGGCACTACACCAATATCATACAAAAAACGATGTCAAAAACGCGAATATAGTAAATGTAAAACAGCATGTTCTTGTCCACCAATATAAACATCAACTGGCAATCATTTAGCAAAACGCTTTTTAGCTTCTTCACTATTTAATGGTGTGTATGAACCATCTTCATTTTTTAAAATATAAGCTAAAAAATATCATGATGAGCCTGCTCATTGTGGCATTGTATTTGTTTCGCGACGATATTTTTTACCATCAATTTCTACATATAATCATTCTTGGGCATTTGCAAGGGGAGATTCACCAGTTTTAGAAGGTTTAAATTCATTTAAATTTGGTAATAAAACAGGTAAGTCTTCGATAATCTTTATTTGATTATTTTCATCGAATAAAACTGGGAATGGTTCACCTCAATAACGTTGACGACTAAAAATTCAATTACGCAATTTATAGTTTGCAACTTTTTTTCCTAGATGATTTTTAATTAAATAATCATTTAAAACATTTTGAGATTGTTTAATATTTAAACCATTAATCATTGCTGAGTTAATATGTAAACCATCACCTGCATATGGTAATTTTTGTTTTGTATCAATTACACTTTTAATTGGTAATGCATATTTTTTAGCAAAAGCATAATCACGTTCATCATGTGCTGGTACACCCATAACAGCTCCAGTTGCAAAATTCATTAAAACATAATCACTTACTCAAATTGGAATGATTTCTTGATTGATTGGATTAATAGCATATGCACCACTAAAAACACCAGTTTTATTAGTATCAGCAATGCGATCTAAATCGCTTATTTTCTTTGTTTGTTCAATATATGATTGAACAACATTAATTTGTTGCTCACTAGTAATAGATTTAAGAATTGGATGTTCTGGTGCAACTACTAAGTATTGTACCCCATAAATAGTATCAATTCGTGTAGTGAACACATCAAGTGTTAAAAGTGAATCTTTAATCTTAAATTGCACACTAGTACCGATTGATTTACCAATTCAATTACGTTGCAACGATTTTAATGATTCACTAAATTCAACTTCATTTAAACCATCTAATAATTTATCAGCATAATCTACAATTTTTAAAACTCATTGTTTCATTGGTTTACGAACAACAGGATGACCACCTCGCTCACTTACCCGTTCGTTTTTATCATTTAAAACAACTTCTTCATTACTTAAAACAGTTCCTAAATTTTCGCATCAATTAACATCAATATCTTGAATTTCAGCTAAATTATGTTTATATAATTGCACAAAAATCCATTGCGTTCATTGATAAAATTGAGGATCGGTTGTATCAACTTCTTTATCATAGTCAAAATCAAAACCAATCATTTGTAATTGTTTACGAAAAATTTTAATATTTTGTTGCGTAAAAGTATGAGGGTGATTATTTGTTTCTAATGCATACTGTTCTGCTGGTAAACCGAAAGCATCTCAACCAATTGGATGTAAAACATCATAACCATTCAAACGTTTAAAACGACTAATTACATCAGTTGCTGTATAACCTTTGGGATGACCAACATGCAATCCTTTTCCACTAGGATATGGAAACATGTCTAATACATAAAATTTCTTAGGATTGTTAGGATTATCAACAAATTTAAAAGTCTTATTATCTAGTCAATACTTTTGTCATTTTTTTTCAATTTTATTGTGATTATACATAAGTCTTACCTTTCTTTAATTAAAATGATTTTTACTTAAATAACTTTCTAAAATAACAACAGCACTCATTTTATCTTTAACTTTTTTGATTTTAGCTGCTTTAACACAACTATTTTTTAAACGTTGAGTTGCAATTTTAGTGCTAAAACGTTCATCCTCATAAACAATTTTAATTTTTTCATGGTTTAAAAAGTGTTGTTTTAATAATGCATAAAATGATTCGATTAAATATGTTCGTTCATTTTTTGATCCATCATAAACATTAGTTGGATAACCTAAAACAATTGTGTCAATTTCATGATGATAATTAATCATGATTTCATCAATTTTAGCTAAAACAGCTGTAAAATCATTTTCTTCATAAATAAAATTATCAAGTCCGCTAGCAATTATTCCTAATAAATCACTAATTGCAAAACCACATGATTTTGTTCCTAAATCAAGTGCTAATTTACGCATTAATAACTCATTTATCTACAACAGTTAATAACTCATCTAATTTTTCTTGTTCACTTGTTCCACCTTGAACAAAATATGGTTTGCCCCCACCTTTACCATTAGAAATAGTATTTAATTCTTTAATATATTTATTTAAATTAATTTGGTTTGTAGTAGCAAACTTTTCATTAATTGCTAATAAATATTGAATTTTATTATCATCTAAGTTGAAACTAATAAATAATGTATCTTGATTTTCGTTAATTAATTCATTTAAAGCATTGAAGATATTTTTATTATCAGTGTTTTTAAAAACGTGTACACGTTTATTAGTTAGTTGTGTATTAAAAGTGTTTTTAATATCAATAATAGCTTGTTTGGCATTTTGTTTATCAAATTCGATTTTAAAATTAATTAATGATTGTTTTAGTTCAGCAAATTTTTCATTTAATAAATCATAATCATCAATTGATAGATTTTTTCAATTTGCAAAAGCATTATATAAATCATTTAATTTATGATCATTAATATCTAAATTAACTTTTAAGTGATTGATTTCATTTAAGTAGTCTACATATAAATCATGAATGTATTTTGCTAAATTATCATGACCTGTTACCATAGTAATTCTTCAAGAGCCAGCCCCTTTAGAAGCACATTCAACAATTTTAATTCGCTCGATTTCTGATGTATGATGTGCGTGTGTACCCCCACATAACTCAATTGAAGGTCCCATTTTAATTACACGTAATTTGCCTTTTAATTTTTTATAAACATCTTCAAAATAAGCAGCAGCACCAACAGCTTTAGCTTCATCTAAATCCATGTGTAATTCTTGTACATCAAAATTACTTTGTACCATTTCATTTAATACATTTTCTAACTTATCTAATTGTGCTTTTGTTGGTTGAGCATGATAAGTAAAATCAAATGTTACTTTATGTGGATATTTAGCAGCTCCTTCTTGTTTAATATCAGGACTTAAAACATGTTTTAAGCAATAGTGTAATAAGTGTTCGGCTGTATGGTTTGCTGTAATTGATTTACGATCTTGCTCGTTGATTTGTAAAATAACATGCTCATTCATTGATAAACTAGCATTTTTAACATGATGAACGTGTTGACGATTAGGTGCCTTAAAAACATCATCAACAAAAAATTGTTGATCATTTTTAATAATATAACCTGTGTCATGTTGTTGACCACCTGATGTTGCATAAAAACATGTGTTATCAAAAACAACATAACCATCTTCATGATCTAAACCATCAACCAATTCAAAATCCTCATTAAATAAAGTAATGATTTTAGCATTTTCTCATCTAAATATTTCATAGTGAAAGGTTGAATCAACATCTAATTGCATTAGTGATTCGTTTTGTGATTCCATTACTTTTAAATTAGCTTTGTTTGCTTTTGAAATTAATTGGTGATGTTTAAACATAGCATCAAAAGCTTCTAAATCAACTTTAACATTACGTTGTTCACAAATTTCTTTAATTAATTCAATTGGAAAACCATATGTATCAACTAGTTTAAAAGTAATTTCTTTATCTAATTCTTGATTACGAATAGAATTTTCAAAAATATTTAAACCTAATTGTAAAGTTTTTTCAAATAATTGCACTTCTTTATTTAAAATCATTTTAATTTTAGTAAGTGTTTTTTCATCTTTTAAATAAGTATAAAAATCACCCATTGTTGAAGCGATAGCGTCAACAACTGCATCAACTCATAAAAGATTGTTAATATTTAAACGACGCACTAAAACCATAGTTCGACGAATTAAACGACGTAAAATGTAACCTCGTTCTTTATTTGAAGGAATTGCACCATCTGCAATCGCAAAAACAGTTGCACGCATATGATCTGCAATTACTTTATAAGCGAAATTAATTTTATTTTGAGTAACATTGGGATGAAAGTATTCATTCATGTCATATTTATATTCTGAAAACTGTTCAACACTTTTTGTAATTCGCATAAAAACATCGCTATCAAAGTTAGTTGGTACATCTTGTGAAATACAAGCAATTCTTTCCAAGCCAGCTCCTGTATCGATATTTTTTTGTAATAATTCAGTGTAATTGTTATGGCCATCATTATTAAATTGTGAGAAAACAATATTTCAAATTTCAACATAACGATCATTTTCAATATCTTCTAAAATTAATTTTGATCCTATTTTATTTGGGTCAAAACGTTCACCACGATCATAATGAATTTCAGTACATGGGCCGCAAGGGCCATTACCTACATCTCAAAAATTACGATCACGATTGCATTTAATAATTTGCTCTTTAATAATTCCTAATTTAATTCATTCATCATTCGTAAATTGATCATCTTCAAAAACAGTTACTCATAGTTTTTTTACATCAATTTTTAAAACGTTAATTAAAAATTCATAAGCTCAATCAATAGCTTCTTTTTTAAAATAATCACCAATTGAAAAATTCCCTAACATTTCAAAAAAAGTATGGTGACGGCTCGTTAATCCAACATTAAAAATATCATTTGTACGAATAGCTTTTTGAGAATTAACTAAACGTTTATGTGGTGGTTTAACCTTACCACTAAAATAATCTTTTAAGGTTGAAACACCAGAATTAATTCATAATAGTGTAGGGTCGTTTTTGGGAATTAATGACTTTGGTTCAATAAATAAATGATCCTTTGACTCAAAAAATTCAATTCACTTTTTTCTAATCTCATTCGTACTTAATTTCAGCATAATAAATATTCTTCCTTACTTTTTTATTATTTAACGCATTACTTTAATAATTGATGGTGCAATTTGTTTTGGTGTTTTTAAATAAACTACATTGTTATGGTATTTATTAACAACTTCATCATTAATTGGCTCGCTAGCATAAAGTGTAGCAATAACTTCATTTGTTTTAACGAAATCATTATTTTTCTTATTTAAATAAATTCCAGCTTGAAAATCTAATTGATCAGTTTTAACTTTACGTCCTGCTTTTAAATCAACAGCAATCATACCCAATTCTTTTGTTGATTTAAAGTCTAAATATCCATCTTGTTTTGCTAAAATCTCTAATTTGTATTTAGGGTTAAAATATGTATCATTTTCAAGACTAATATAATCACCTTTGTATCATTTTACTCAATTCACAAATTTTTGATAAGCTAAACCATTTTTAATGACGTTATCAATCTTAGATTGAGCATCTTCCTTGTTTTTAGCGAATTTTGTATCAATTAAGATGTCTAAAGCAAACTGATTAATTAAATCATATAAATCTTGGGGCACTGGTTTACCTAATAAATAATCCATACTTTCTTTAATTTCAATAGCATTACCAATTGCTTTTCCTAAAGGTTGGTCCATATTTGTTAAATGGATAATTGTATTACGATTAAATTTCTTTGATAATTTTAACATCTTTGTTGCTAAAGATTCTGCTTGTGCAACATTTTTACAAAATGCTCCTTCACCAACTTTAACATCTAAGAAAATATAATCAGTAATTAAAGCTAATTTTTTAGCCATAATTGATCCTATCATAAGATCAAACACATCACTTGTTGCTGTTACATCTCGTAATGCATAAATATATTTATCAGCCACAACCATATCATCTGATTGACCAGTTAATAATAAACCATTATCTTTTAGCTGATTCATGTATGTTTTTAAATCAAAATCTAAATTTAAATTCAATGATTCTAATTTATCAACTGTACCACCAGTATGCCCTAATCCTCGACCAGACATTTTTCCAACGTATAATCCTAAAGCTGATAGTAAAGGAGTTAAAATAATTGAAACTTTATCTCCCACACCACCAGATGAATGTTTGTCAACAACAATTTTATTTTCAACACGCAAATCTAATAATTTACCAGATTCAACCATTGCTTTAGTTGCATAGTATTGTTCATTTTCACTATATCCACAAAAATTAACAGCCATTAAAAAAGCTGCCATTTGATAATCTGGAATTGTCTTATTAACAAAACCATCATAAACAAATTTAATTTCTTGTTCACTTAATTCAATATTATTTTTTTTCTTGCTAATAATTTCTACAACATTTAACATAAATTTCACCAATTTAATTTTTAAAAACTTATCTTTAATTTTAGCAAAGAAAAACACGAAAGTTTTTAAAATAAGTGAGTTTTACTAATCTATATTAATAAATTAAGGATTAAGATTTTAATTATGAATAAACATTAATTATTAACTAATGTACCAAAAATAAATATTTATTTAAAGATGATATTTTTTAGAACACATTATAATAAAAGTATGATCCTAAAATAACACAAAGGAGTCGTTTATGAAAACTACAGCTAATAAAAAAATTAAATTAATTAATATTGAAAAATTAGAATTTGAAATTCTTGAGCCGATTAATACCAATGAGATTTTGGTTTTCAAAGATGAAAATTCAACTATTTATGAATCTTTTAATCAAAATTTAGAAAAACAACGTGATGAATTTATCAAAAAAATTCGTGAAGAAGAAGAACAAAATTGAAGAAATAATTTTTTAGCTAATGATCGTGATTACAATGAGTTAGTTAAACAAAGATATGATTTAGAAAAACAACGTGATGAATTAAAAGAAAAATTAAAAAATGAGGGAAATAAAGCAATTGCTCATTTTAAAGATAGTGATGAATACAAAAATTTAATTAAAGCACAAGAAAAAATTAATTCTTTAAATAAGACAATTGAAAGTAATGAACAAAGCTACAAAAAAGAAATTGAAAATATAGAATTAAAGTTAAAAAGCCAATTTGATGAAGAAACAAAATCGTTAAAAAATACTATTGCTAAACAAGAAATTAAATTAGATAATGCTGAAAAAATGGCAATTATTAATTTTAAAGAAAGTAATGAATATCAAAAAATTATTAAAGATAAAATAGATTTAGATATTGAAATAGAAAAATTAAAATTTGCTATTCAAGCTCATGAAGATAATATGAAGGCTGCTAAAGAAAATTGAGAGTCTAAAAAAATTGTTGAAATTAAGGAACTTGAAAGTAAAAAAGATAAAGAGATCCATAAATTAACAGAAAGTATTGAACAATTAAAACGTGAAAAATCATCAAATGTTAAACTTGTTGGTGAAGAACTTGAACAATGATTAAAAAATAAATTTGATGAAACTTACTCATTTTCATGTCCTGATATGACATTCACTAAAATTAATGAAGCAATTGATGGTAAAAAAGCTGACTTTTTATTAGAATTTTTTGATTTTGGTAAAGAAATGTCAAATGATGATAAAAAATTAATTTTTAGTGCGACAATTGAAGCGAAAACAGAATTTTTTGACAATCAAAAAGGTACAAAAAATAGTGCCCACTATAAAAAATTAGATCAAGATCGTATTAATCAAAAATCTGAATATGCAATTTTAGTAACTGAATTAGAACCAGAAGATCATTTTGTAATTAAAAAAATTAATGAATACAAAAATATGTTTGCTGTACGGCCACAATACTTCATTCCTTTAGTTGATATGATTCGAAATTTTGCAACCCTTAAAGCAAAAATTAATTCACAAATCATAAGATATGAAGATCGTGCAAAAATTGAAGAAAATCTTGATGAATTAAAAAAAGATATTGTTGATAACACTCTAAAATACATCAATGATAAAACTAAAAAAATAATCGATGATTCAAAAGCTATTATTAAAAAAGCAGAAAGTATTGAAGAAAGTGCTGAAGATATTATTAACAAAAAACTAAATACTTTAAAAAAGAAAATCAATGAATTAACAATTAGAAAAATTGATCCAAATAAACTTTTGTTAGAAGTTAGTGATGATGAAAATGATGAAGAATAAATAAATTTCATAAATTATTAATTCTTGATGATCATAACCACATTTCAATTCCTTATATTATGTTAAAATTATTAATATTTAATAATATTTTAATGTTATATTTAAATATAAATGTCCAAGTCGTTTAGTGGAGGAACAAATGTCTGCAACTAATAAAAAATATGATCCACAAATTAGCGAATTATTAGAGCTTCGTGATTTAGCTAATAAAGTTGATAATTTAAAAAGAAAATTAAAAACAATTGAATATAACCATTATTGTGAACACGAAAATTATGATAGTGATGATGGTGAAACTAATAATGTTTATGAACAAGAAGTGTCATTAAATCATGAAACACAAATTGTTAAATTACGTGTTGATTTAAATGATTTAACAAACCTAAGCAATACAAAAATTGTTGAAGTTGAAAAACCTGTTTATATTGAAAAAGTTATTGAAGTTGAAAAGCCAATCGAAAAAATTGTTGAGAAAATTGTTTATGTTAACCAAGATAATCAAGTTAATAAACAAGAAAGTATTAAAGAAGAAAAAGAACCAAAAACACCTGTTAAATCTGGCAAATACGTTAAATATCGTCGTTTAAAAAATAATGGTATGTCAGATGCTGGTTGAAAGATTTTTGAATTAGTAAAACAAACTCAACAAGATAAGGTTGCTGCTGAAAAGCGTTTTGATGAACAACAAGAAGAAAAGAAACGTCAACGTGAACAAAAGCGTCTTGAAAAGCAACAAAAACTAGAAGCAAAAAACGAGCAATAAAAACTAATTTAAAGCGAAACTTATTAATCAATGATGTTTTGCTTTTTTATTTATATATTATTTAAAAAAGAAAAAAAGGTAATGATTTTATTATGCAATTAAAAATTAAAGAAATTTTTAATCAAGACTATGTAAAACTTGAAGGCCAAAAGATACAAATTAAAGCTTGAGTTCGTTCAAACCGTGATTCAAAAAAAATTGGTTTTTTAGTTCTAAATGATGGTAGTTCTTTAACAAATTTACAAGCTGTATATCGTGTTGATAAAATTGACAATTATGAAGAAATAGCTGCAGCACGAATGTGAGCCGCTGTTGTTATTGAAGGTGTAATTAAATTAACTCCAACAGCTAAACAACCATTAGAACTAGAAGTATTAAATGCTCAAATTTTAAAACAAAGTGATGAAGATTTTTTATTATCAAACAATGATTTAAATTTAGAAACATTGCGTTTAAATGCTCATTTAAGACCAAGAACAAATTTATTTCATGCCATTATGAAAGTGAGGGCAACACTTGCTTTTGCAGTTCATGAATTTATGAATCAAAACGAATATAGTTGATTAGCTGCACCATTGTTTACAGGAAATGATGCTGAAGGAGCTGGCGAAACTTTTAGTATTCAAAAATTTGATAATGAAGAATTTTTTGGTAAACAAACTCATTTATCTGTAACAGGTCAATTACAAGCTGAAGCATACGCACAAGCTTTTGGTAATGTTTATACATTTGGTCCTACTTTTAGAGCTGAAAAATCTCACACAAATCGTCATTTAGCAGAATTTTGAATGATTGAACCTGAAATGGCTTTTGTTGATTTAAAAGGTATGCAAGATATTGTTGAAGATTTAGTTAAACATGTTATTAAGGCTGTTTTAGAAAAAAATCAACAAGAATTAGAATTTTTAGCTCAAAGAAATGATGAAAATTTAATTAAAAAACTACAAAAAGTTGTTGAGTCTAAATTTGAACGTATTGAATATAAAGATGCAGTTAAAATCCTAGCTGATGCTGTTAAAAATGGTCATCAATTCGAAAATAATGAAATCTTTTTTGGAATGGATTTAGGATCAGAGCACGAAAGATACATGTGTGAAACATATCATCAAGGTCCTGTGTTCTTACAAAATTATCCAAAGGATATTAAAGCATTTTATATGAAATTAAACGATGATCAACAAACTGTAGCTTCAACAGATTTATTAATTCCAGGCGTTGGCGAATTAGTGGGTGGATCACAACGTGAAGATAGTTATGAGAAACTATTAAAACGATGTCAAGAATTAAAAATGCCAATTGAAAGTTTACAATGATATTTAGATTTACGTCGTTTCGGATATTATATGTCATCAGGTTTTGGAATTGGTTTTGAGCGTTTAGTGATGTATGTAACAGGAGTTAATAATATTAAAGATACAATCCCATTCCCACGAAGTCATGGCCAAATTGAATTTTAAAAAAAACATCTCATCATCACCTTTTTTTAGAAACATTCCTAATATTATCACTATCTTTAGAATTTTTTTAGCACTTATTTGCATCATCTTATTATTAGTTGATTTTTACACAAAAAATTTAAATATTGTCTATGAAGTTTTAGATGCCAATATTAGTGCATTGCGTTTAAGTGCAACAATTATTTTTATTATTGCTGCTTTTTCTGATTTCTTAGATGGATATATAGCTCGTAAATATAATTTAGTTTCAAATTTAGGGAAAATCTTAGATCCTATTTCTGATAAAATCCTTGTTAATGGTGTTTTAATTTGTTTAACACTAGATCATACAGCACTAGCTTATTTAACAATTATTAATATCTTACGAGATATTTTTATTGATGGTTTACGAATGTTTGCTTCAAGTAAGAAAATTATTATTCCAGCAAATATTTTTGGCAAAATTAAATCCATTCTACTATTTATTAGTATTTGCTTTATTTTATTTTTACTAAGCTTAACCTCATCATGAAAAAACGTTTATTTATTTAATATTCCTTTATTTTTTGCAACATCATTATCTGTAATCAGTGGTATTATTTACTACATTAATTTTTACAAAGGAGTTAAAAAACGTGGATCAATTACAAAAAGCTAAAATTAATCAAACACAAAAAGCAATTGTAGAGATTAAAAATTCTTTTCAAAAACATTTTGCAAAAAATTTAAATCTTTCACGTGTAACAGCACCATTATTTGTTGAAGGACAATCAGGTCTAAATGATCATTTAGACCATAAACAAAAAGCAGTAAGTTTTTATGCTAAAAAATTAGATAAAACACTTGAAATTGTTCAATCACTAGCAAAATGAAAACGTTTAGCTTTATTAGATTATGGTTTTAGTTTATATGAAGGTTTGTATACTGATATGAATGCAATTCGTGCAGATGATGACATCGATGAAATTCATAGCATTTATGTTGATCAGTGAGATTGAGAAATTTTAATTAATAACCAAGATTGTAACTTAGATTTTTTAAAATCAATCGTTAATAAAATTTATTCAACAATTAGAATTGTTCAATTAGAAATTGATCAATTATATAATCCAAAACAAATTATTTTACCAGATAGTATTACTTTTATTAGTTCACAAGAATTAGAAGATTTATATCCACATTTATCACCATCTCGACGTGAATATGAGTTTGCAAAAATTCATAAAGCAATTTTTATATACCAAATTGGATATCCACTAAAATCAGGTTATATTCAATCAATACGTTCTCCAGAATATGATAATTGAAATTTAAATGGAGATTTAATTGTTTATCATAAATTAAATGATCAAGCCATTGAATTATCATCAATGGGAATTCGTGTTAGCAAACAAGATTTTATTAAGCAAACAAATTTTGCTAATTTAAAAAATGATCAAGAAAATAATTTTTATCATCAAATGATTTTAAATAATCAACTACCCCAAACAATTGGTGGGGGAATCGGTCAAAGTCGTTTATGTATGTTTTTATTAAACAAAAAACACATTGGTGAGGTACAAGTTTCAGTTTGACCAAATGAATATAAAGATGAATTGTTAAAAAAAGGGATTAAATTACTATAATTTAATTTAAATAATAAAGGAGGTTTTTAATTTGAACTATAAAACATTAATTAATGGAGCATTTGTTGATGCTAAAGAAAAACTACCAGTTTATAATCCATCTAATAATCAAATTATTGCTTATGTTCCTAACATTCATAATGAAAATGAAATTAATACTATTTTTGAAAATGCTCATATAGCATATCTAAAATTTAAGGACACACCAATTAAATATCGTTGTGATCTACTTTTAAAATTAGCCGATAAACTAGATGAACATAAACAAGAATTAGCACAAATTATTAGTACTGAAATTGCAAAAGGATTAAAAGATAGCCTAATTGAAGTTGAACGAAGTGCTGATTATTTACGCGAAACAGTTTTTGAGTATCAAAAATTAATGCAAAAACCAATTATTTTTGATGAAACAGTTCACCATGTTAAAAACAAAGTGGCAACATATTATCGTATTCCAGTTGGTGTTGTTTTAGCAATTTGTCCTTTTAATTATCCTATTAACTTATTGATTTCAAAATTAGCACCAGCATTAGTTTCTGGTAATAGTTTAGTTTATAAACCATCAACACAAGGTTCATTAATTGGTATTCGAATTAGTGAATTAGTTCATGAAGTAGGTTTTCCAAAAGGAGTTGTTAACTGCTTAACAACTGAAGCACGTATAACAGGTGATCTACTAGTTACTAATAAATATGTTAAAGCGATTAGCTTTACGGGCGGACCTAAAGTAGGCAATCATATTGCTGAAATAACATCAAAGATTTCTTTAGTTTTAGAATTAGGAGGAAAAGATCCAGCCCTTGTTTTAGATGATGCGGATTTTGAACTAGCTGCTAATGAAATAGTTAAAGGTGCTTATGGCTTTAGCGGACAAAGATGTACTGCAATCAAACGAGTATTTGTTTCGCATAAAAATCATGATTTATTAGTTAATTTAATTAACAAAAAAGTTGACGCTTTAACAGTCGGTTTACCACAACAAAATCCCATAATTACTCCATTAATTAATTCTAATTCATTAAAATATAACTTATCATTAGTTGAGGATGCAATCAAAAAAGGAGCAATAGTACACCAAAAAATCGTTTATAATGAAAAAAATAACCTTTTGCATCCTTTAGTAATTGATAATGTTACAAAAGAAATGCGCGTAGCTTGAGAAGAACCATTCGGTCCTATTTTACCAATTATAACTTATAATAGTATACAAGAAGCTATTGATTTAATTAATCAATCACAATATGGTTTACAAGCTTGTATTTTTACAACAAATTATGCAAGTATTGAACAATTAGCTTTACAAATTGAATCAGGAACAATTAACATTAATAAATCAAGTTCACGTGGTCCAGATATTTTACCTTTTTTTGGTGTTAAAGATTCTGGATTTGGTGTTCAAGGTATTGTTGATGCAATTTTATCAATGACAACAATCAAAGGAATAATCATTAATAAATAAAAACCTCCTTCTTTAAAAAGAGGTTTTTATTTATTAACAAACTAAAGCATTTTGATAGGAATTGGTTGAAAATGTTTAGCATTCATTATTAAGTTATCTGTATCAAAATAAAATGATTTTAGTAAATCATCACCAACAACTAACATTTTAGTATTAGCTAATAAAACAGGAATCTTTACGTAAATATCAACATATTTATTATTACTTAAAACTTGTACAGGTAATGCGCGCATGCATAAATCTTGTTGCTTAATATTTTGTTTTGCGTTTTCTTTTGGAATAACAGTTTCAACATTAATATAAGTTTTTTTAAGTTTATTAATGAAAGTATTTCATTTAAGATCATCTTTAAAATACTTTTTATTTTCTTCATCATTACGACTAATACGAAACTTAACAGTTTTTTCATTATTCTTAGTGATATAAATCTGATCATCTGCAAATGTATTAGGATAAAAATTTTTCTTTACACAAGCAGTAGCAACTAATGTAATACTTCCAAGACTTACAAAACTTATTGGTATTAATAACCAAAATTTATTTTTTCTAAAATACTTATTTTTCATTAATTAATCCTTATTTTTATAAAACTAATATATATTCTACTTTAATTCTTTTTGTAAGAATTAAATTTTTAGATAAACTCTAAAAATAATAAATAAAATGTGAACGCTTTCTTATAAATATAAAGAAATAATTCACATTTTTTATAATTACGAAGGATTATTCATCAGTATTTTTAACATTTAATTCATCTCTAAAAGCACGTAAATGTTTAACATTATCCTTAGTAAAATTAGGAGTACCCAAATCAACTTCTGGACTATTTTCACCATTCATTCAACGATTTAAAATATTAACAATTGTTTTTTGACCCATAAAACCATAACAACCATCGCAGAATGTTCCTTCACGTTCAATAAAGAAATTTTTACCAACAACTGGCTTTTCTTTTAAAATCCTTGTAGGATTAAAACTATTTTTTGTATTTATATCTAAACTATTTACATATTTTGCTAAATTAGCTTCGAATTTTTTAATTACAGCTTCACCTTCTGCTGTTCCTGGTTTATATCCTTTAATTAAATATTCATTATATGAATAAATTACTTTATCAACCGTTCCTTCAAAAGCTTCACCCAATGTTGGACCAACACGTTTTGCAGCATCAGGATTTTCTGCACTAGATGATAATATTGCACCGCCTATTCAACCTCAATTATCAATATATTTTTGTACATCTTCTAAGTGTTTTTTAGGAATAGGGAATTTACAACCCATTCCAGGTGTATAAGGATGATTTTGATCAGCATATAAAATTGGTAAATAAACGGGTTGGGTAATAAAAAAATTACTTAAATGTAATAAATCTTCTTTAGTAATGTTTGGATCTACAATTCGACCATTTGGCATTACATCAGGACTAATCATAACCGTTGTTTTATCTTTTCATTTATTATTTTGTTTTGCTCATTGTGAAACTCTTTTTCGAACAGCTTTTACAGCAACATTAGCATAATCTGTAAATGTTTGAAAATTGCTTTTACCATCAATAACCGCTTTATTAACATTATCAAATTTTTTTGCATCATAAATGCGATCTAAGGTTTTACCTGTAAAGATTAAGCCATCATATGGATCAACAAAAAAATCTGTAATTCCAGCTGAAGCATTAACATCAGTTTCTAAATAAGGATTATTTTTACCAAACAACCAGCTAGGCATGAATAAGGCTGGTGTTTTTGAAGGCAAACCTGATGATCGTGATTGCATTGCTACACCACCAATTTCATTTTTATCTAAAAAATTTTCTTTATACTTATTGAATTTAAAATCAGCAATAAATAATGTACCCACATTTAATTTAGCAAAATTCTCTGGTAATGCTCCTTTACCTGTGAAATTCATTCCCTTTGTTAGTTTAGAGTTGTATAAATAATTTAAATAATGAGGATGTACTTCTTCAAGTTCGCTTGTTTTTCAACGAAAGCCAGAATAATCAGGTGCGATTCCAAATGCATATAGTGCATCATCTTGACATAACGAACTAGAATCATTAGTAACCGCAATTTTTTGGTTTTTTTGATAATAAGAACTAGCTTTTGATGATAAAGTTTCAATTCGATTAAATTTTTTTCCTGACTTACTTACTCCACCTTCAAAATAAACTTCTAAATCAGCTTTTCATGCTTCTTCTCTTTTTTTAAGCTCAGTCAATTCTTCTAAAGTTGGTGTTGTTTTTGAAAAATTTGTTTTAGATTTTACTTGATTGCTACATGCAGCAATTGTAATAATTGATGATAAAATTATTAAACTAGTTGATATAGATAATAAGATTTTTTTAAACTTCAACTTCATATATTTTTAGTAATTCCTTTTTTAAGTTTAAATGATTAATTAATAAATTTAAAAAATTTATGCATAAAAACTTCCAATGCGTAAAATTTTTATTATTCATCAGTATTTTTAACATTTAATTCATCTTTAAAAGCACGTAAATGTTTAACATTATCCTTAGTAAAATTAGGAATACCCAAATCAACTTCTGGACTATTTTCACCATTCATTCAACGATTTAAAATATTAACCATAGTTTTTTGACCTATAAATCCATAATTAGCATCGTAAATATTACCTTTTCTTTCGATAAAAAAGTTTTTACCTACCACTGGCTTTTCTTTTAAAATCCTTGTAGGATTAAAACTATTTTTTGTATTTATATCTAAACTATTTACATATTTTGCTAAATTAGCTTCGAATTTTTTAATTATAGCTTCACCTTCTGCTGTTCCTGGTTTATATCCTTTAATTAAATATTCATTATATGAATAAATTACTTTATCAACCGTTCCTTCAAAAGCTTCACCCAATGTTGGACCAACACGTTTTGCAGCATCAGGATTAGTAGCATCACTTAAAATTGCTCCACCTATTCAACCTCAATTATCTACATAGCGTTGAACTTCATTTATATCTTTTTTAGGAATAGGAAATTTAGCTCCTAATCCAGGTGTATATTCATCATTTTGGTCTGCATATAAAATTGGAAAATAAACAGGTTGTGGAATAAAAATATTACCTAAGAATAGTAAATCATCTTTACTAAAATTAGGATCAATAATTCGTGCTCCTTTCATTACGTTTGGCATTGTCATAACAACAGTTTTATTTTTTCAATAACTATGTTGTTTAGCTCAATTCGAAACATTCTTTCGTACAACACTAACAATTGCTTTTGCATAATCTGTAAATGTTTGAAAATTGCTTTTACCATCAATAACCGCTTTATTAACATTATCAAATTTTTTTGCATCATAAATGCGATCTAAGGTTTTACCTGTAAAGATTAAGCCATCATATGGATCAACAAAAAAATCTGTAATTCCAGCTGAAGCGTTAACGTCAGTCTCTAAATAAGGGTTATTTTTACCAAATAACCAATTAGGCATGAATAAAGCTGGTGTTTTTGAAGGCAAACCTGATGATCGTGACTGCATAACAATTCCACCTATTTTATTTTTATCTAAAAAATTAGTTTTATAATTTCCAAATTTAAAATCTGCAATAAATTAAATAAAGTACCTACATTCTCAGCAGCTAATGTTTCTGCTTTAACACCTTTAAGTTCCATACCTTTAGTTAATTTAGGATTATAAAGGTAAGATAAATAATGTGGTACAACTTCTTCTACATCACCTGCTTTTCAACGATAATTAGAAATATCTGGTGCAATTCCAAATGAATATGCGGCATCATCTTGACATAATGAGCCAGAATCACGCGTCATAGCAATTTTTTGGTTCTTTTGATAATATATACTTGCATTTGTTGCTAAAGTTTGAATTCGCTCAAATTCTTTTCCTGAAGCAGACTTTCCACCCTCAAAATAAACTTGTAAATCATTAGCTCAATCCTTACGTCTTTTAATTAAATTTGTAAGTTCTTCTTTAGTTGGTAATATTTGTTTAAAAATGTCATTTGAAGACTTAACTTCATTATTAACTTTACTACAAGCTGTAGTTGTACTTACAATAGTTGTTATAAAAGCAATAGAAGCTATTGAAGTAATTCAAATTTTTTTATATTTCATAACAATATTCTTTCTTTATTTTTTATTTAAAAAAATTATTAATACCTTTAATTTTAATACACAAAAATAAATATATTTTTTTCCATTTACCACCCAAAAAAAAAAAAAAAATAGTGAACAATATGGCTTGTTCACTACTAAGATCGTGCTTAAATATTATTTTTAAATAAGATAATAAATGTAAATATTGGAGCTACTAATAAAGGAGTTACAATAGGGGCGTCAAATACCAAACTAGTTAAATTTTGAACGATATTATCTAAAATAAATTGACCAAAAATTAAAAAGATAATGGTGATGAAGCTTGAAAAAATTATTGATGAACTAAAGCGATTACCAAAAACCGCATACCCTAAATTTGCTGCAGCAATTCCTAAAAATAATACATTTCCTCCCATAATATATGAAACAGCTGTTAAACAAGATGAGATAAAAATCATTTCTAAATTCAATAATTTTACTTTTAATCCTAATTGTTCAGCAATCATTGTATCTGTAATAACAACTTTAAATTTAGCAGAACGCAAAAAGCATCATATCATTAAAAATAAAAAAACAGCATAACTTAAATATTTTTCAGTACTATTACGGTTTGAAAAATTACCTAACAAACGATCGTCAATATAATTTGATGCAACTAGAGTTAAATTGTCTTTTAAGCTAATTGATAAAGCAATAGCAATAAAATTAATGAATACACCAGAGACAATTAACTTTGTATGGCTAAAACCGGTTCGTTTTTTTGAAAATCAATACAAAATTAATCCGGCACATACTCCTGATATTATAAAAATAAAAGGTAATAAATTTTCAAAAAGTTTTTGAGAACCATAATTTTCAAAATTAATTGATATTGCTAAAAAAAATAACCCAATCAAACAAACATTACCTATCCCAAGAGTTGATGTATCAACTAATCGATTTTTAGTAATCCGTTGGAACATATAAGCTGATGCTCCTAATGATGCTCCTGTAATTCAAATTTGTAAGATTGTTTCATAATTTACTTTAAACAAATCTAAAGGAGTCCGATTATTTAATAATTGACCTTGGTTATTAATTTTAATAAACATAACTAATAACGATAAAAAAAGAATTGTAACAATACTAATAACTCAAGCTGCTAAATGTTGCTTGCGTGCATAAACCCGAAATTTTTTCTTGAAATTTCCATTTTCAATAAATGATGCATTGTTAATGGTATTTAAAATATTATTTTTTATTATAAATATAAATCGTGATGGATTTTTCATATTATTTATCCGTGTTTTCGCATAATAAAAATTAACACAAATGCACTAACAATAGCCATTAAAAAGTTAGGACCAAAACGATAATTTAGTGATAAAAATAATGAAACTTCTAACAATGCTAATGAAAATACAATAGATACAAAAAATACAAATAATGGTTCATGTTTATTAAATGTTGCCCTAACTATATTTGGAATAATTAAACCTAATAGAATCATGGGACTTGTAATATTAACAGTTGCAATTGTTACAATTGCTGTTAAAACTGTCACGACTCAAAAAATTAAATTAACTTTGATTCCTAAAGATTTTGCCAAAGCAAAATCGCGTTGCATAATTTTTAATTTTGGTGATAATACTAAAAAAGCAAATAAACAAATCAAAATTAAAGGCATTGAAATATATAATTTTAATCACTCATAAACATTTGGTAATTCACCAGTTCAAATTGTCACTCGATTTAAAATATTTGGACGTGAAACAGCAATTACTAAACCTACCGCTGTAATTGTTGCAGATATAGCAAATCCAATTAAAACTGGTTTAAATGAACGATTACTAGATTTTTGTCGTTGTAAAATATAATTAACTAAAATGATAAATGATGAAAATATAAACCCAAAAACATATACTAATCAGCTTTTACCTTGATCAATCATCAACATTGCTACATAAGCCAAAATTGAAACTGGAATGTATCCTAATGTTGAAGGTGATGATAAACGATTCTGAGTTGTAATCTGAATGGCATATCCAGCAACCGGCATAGAAATTGCTACAAATAATTTAACGATAGGTGGATACCAATACAATAAAATACTTTTATCATCAGCTACTACTTTAGCTCAAGTTCGAAAATAGGTTACATGAGTATTATAATTCGCATCAAAATATGTAGATTTTTCATAAAAAACATTCATAAAAAACATACCAACAGCTAAGATAATAAATGTTAATACAATAATCGTTATCATATAAGGACGACGAATTTGTGTACTAACAAATTTTTTTGCTTCAATGAGTTTTTTATAAATAGAAAGATCTTTTTTATTAATATTTACTTGTTTTATTTGCATAACTATTTATTTCTCTATTTTTGTTTGTCTAAAACTTTAACACCTAAAGAATCCTTAAATGCTCTAATGTGTTTAACTGTATTTTTATTAAAAATAGGATACCCTAAATCTATATTTTGAGCGTTAGCTCCCCTTGTTCATTTATTTAATGTATTAACTACTAAATCTTGACCAGCAAAACCATAACATGCATCATAGAACTTACCTTTACGTTCAATAAAGAAATTCTTACCCACGACTGGTTTTTCTTTTAAAACTTTTGTTGGCACAAATTGATTTTTTGTTTCTTTTGTTAAACCATTAACATAATTAGCTAAATTAGTTTCAAATTTTTTTAAAACTTCTTCACCTTTAGCAGTGCCTGGTTCAAAGCCTTCAATCAAATATTCATTATATGAATAAATAACAATATCGGTTGTATTTTCAAATGCATCACCAATTGTAGGAGAAATAGTTTTTGATAATTCTTTATTACTATCACCAACTAAAGTTTTTCCACCAATTCAACCATAATCATCAACATAACTTTGAATCTGATTAATATTTTTTAATGGAAGCGGAAATTTTGCACCTAGGCCAGGAGTAAATGGGTCATCTTGCGAAGCGTAAATTCCTGGGAAATAAACTGGTTGAGGGATGAATACGTTACCTACATTAAATTTATCCACATCAGTTTTAATTTCTGGATTAATAAGGAAACCTGCATAATCTTGAGGGTTAGACATCATATTTAAAACTGTTTTACCTTTTCATTCATTATTAGATTTTGCCCAATTTGAAAAGTTTTTACGAGCTATATCAGATGCAACTTGTGCATATTCTTCAAAAGAGTTAAAACCAGTTTTACCATTAATTGTAGCATTATGAACATTGTCAAATTTTTTAGCATCATAAATTCGATCCAATGTTTTACCTGTTGTAATTAAACCACTAAAAGGATCAACAAAATAATTTGGTCGTCCAGCATGATCTGATTCTTTTTCTGCTACATATGGATTTTGATTACCAAATAAAAAATTTGGTTCAATATCAGCCGGAGCTTTTGAAGTATAACCTGTTGATCGTGATTGAATTAATAATCCACCAACTGATCCGCCACTTGCTTCTAAAAGTTTACCATGACTTGCAACTTTTGAATCTGCAACTAATAAAACACCAATATTATAATTTGCAAATTTATCAGCAGTCGCATTTTTAAAACTATCACCTTTTGTTAAATTCTCATTATAATATGATCGTAAATATTCAGGCACTGCATCTTTAATAGTTGCACTATTTCAACGATAATGAACATAATCGGGCGCGATTCCAAATGAATAAGCTGCATCGTCTTGCACTAATGAACCAGAATCACGTGTTGTTCCTAATTTTTGATTTTTACTTAAACGTTCTTTAATCTGTGATGATTCAGTAATAACCTTTTCAAATTTTGTACCTGATGATGTTTTACCACCGTTTAATCAAAACTTTAAACCTTCAACATAATCTTTACGGCGTGATTGTAAATCAAATTTAGTAGTTGGATTATTTGAATTATTTTTTTTAGTATCTTTACAAGAGGTAATTATTGCAGTAGCTGATGCTAAACTAATAGCAGCAACTAATCCACCTAATAATATTTTTTTATATTTTAATTTCATATGTTTCTCCTCAACATTCTATCAATACATTATTTTAATACTTTCACATAATATTATTAATCAATATTGTCCAACCATGAGTAATATAATTAAACTTTTTGTTTTTTTAATAAGATAACGGCAATAAAGATTGGTGTTACTAATAATGTTGTAATCATCGGTGTTTCAAAATATAGTTGTGTCAAATTAAAAATAATATTGCTTAAGAAAAATTGCCCAATTAATAAAAATAAAGTTGTAATTAGTCCTGAAAAAAGCATTGAACGCATAACTTTTTTACCATGGAATGAATAAGCGATATTAGCGGATGCAACACCTAAAAATAAAACATTCCCTGACATAATATATGAAACACCTGTTAATACTGAAGTGATTAAAATCATCTCTAAATTAATTAATTTAACTTTTAAACCTAATTGTTCAGCGATAATATTGTCAGTTAAGACAATTTTAAATTTAGGTGATCGTATAAAACATCATAAAATAATGAATAAAAATATTGCGTAACTTAAATACTCTTCTAAATAGCTTCTTGTATCAAAACTCCCAACAATTCGTGAATCTAAATATTCACTTCCTACTTTTGTTAAATTACTTTTTAAACTAATCGCCAAAGCAATTGATATACAATTGATGAAAACACCACAAATAATTAATTTACGGTTATTAAAACCATGACGTCGTTTTGAAAAAAAGTACAAGCATAAACCAGCTATAATCGAAGAAATTATAAAAACGAATGGCACAATTTTTTGAAATAAATTCTGTGCTTCAAAACCACTTTCAAAACTTAATGATAATACTAAGATTATTAGACCAATTAAGCAAATATTACCAATTCCAATAGTAGATGTATCAACAAGACGATTATTAGTCATTCTTTGTAAGAAAAACGCAGAAATACCTATGGACATACCTGAAATTCAAATTTGCAAAATAGTTTCATAACTTAAAATAAAAGTATCTTTAGGATCTCTTAATTTAACAAAATGGCCGTTTTTATCAATATTAACAAAAATTATAATCAGTGAAAAAAAAGCAATAGCTAAAATGGTAAAAATTCATAATATGATATGATTTTTGTTAAAGTTTGAATATTTTTTAAATGACTTCTTTAAATTACCATTATCAATAATAGGATTTTTTAATTTATCTTTTAACTTAAATCTAAATATTTCTTTTTCAATCATATATTCACTATACTTTCGTGTTTGTATAAATGTTAGTTACCACAATTACTGGATTTGAAAACAAAAAACTATTTAGATTATTTGTTTTTAAAATCATCTTTAAAAGCACGAATATGTTGAACATTATCTTTATTAAATTTGGGAATATTTAATTCAATAGGTCTAGCATTTTCCCCGTTCATTCATTTATTAAATGTATTAAAATTGTGAATTGTTCCTAAAAAACCTAAAAATGCATCATAGAACGAACTTTTACGAATAATAAAGAAATTTTTACCAACAACCGGTTTTTCTTTTAACATTTTTGTAGGATTAAAATTATTTTTTGTGTTAATATCTAAACCATTAACATAATTTGTTAAATTCTTTTCAAATTGTTGTACTAAAGCTTCGCCTTCTTCAGTTCCGGGTTTGTAATTTAATAAACCGTATTCGTTATAACAGAAAACTACAACGTCACTTGTACCTTTAAAAGCATCACCTAAAGTTGGGCCAACTTGTTTTGATGCTTCTGGATTACCAGTACCATTTAATACTTTTCCACCAATTTGTCCATAATCATCAATATAATTTTGCACTTGTGAAATATGATTTACTGGAATAGGGAATTTTGCACCTAATCCAGGTAAATATTTATCATTTGGATCACTATATATTGTTGGACAATACATAGGTTCATTAATGCAAACATTACTCAAGAATTCTAAGTCTTCTGGTTTAAAATTAACATCTACCATTTTAGCTGTTGGAGTAAAGTTAGGCATAACCATTAAAACTGTTTTATCTTTTCATTTATCATTTTTTAAAGATCATTGTGAAATTCCACGACGCGTTTCATCAACCATTGCCTTTGCAAAATCTGTGAATGTCTCAAAATGTGTTTTGCCAGCTACATGTGCATTATAAACATTAGAAAACTTTTTAGCATCATAAATACGATCTAAAGTTTTTCCTGTTAAAATAATCCCATCTAGTGGGTCAGAGTAATAATTAGGTAACGCATAATTAGCAGAAGCATATTTTTTTAAATTTGGATTTAAATTAGGATCAAATAAAAATTTTGGTGTTAGAACAGCAGGATAGTGTGATGGCAAACCAGAAACTCGTGATTGCATAATTAATCCACCAACAGTTTTTTTGCTTAAAAAATTATCATTTCATTTACCATAATGGTGATCAGATATTAATAAAACACCGATTCCATTATCATCCAAAGTCTTTGGGTCTAATTTTGAAAAGTTTCTACCTTCAGTTAAATTTTTATTTCAATATGGTTTTAAATATGATGGAGAAACGTCGCCACCATCTTTTGAAACTCATTTATAATGAACATAATCGGGTGCAATTCCAAATGAATAAGCTGCATCATCTTGTACCAATGATCCAGAATCACGTGTTGCCGCTAATTTTTGATGATTCTTAAATCATTCCGTTGCATTTGATGATAATGTATTTATACGGTCAAAATTTTTGCCACTTTTACTTAACCCACCATTAAAATAAACATCTAAATCTTCACTTCATTGTTGTCTTTGTTTTTCCAAATTTTCTACATCTTGTTGAGTTAAATTAATATTTGGATTTGTTTTTAAATCAGACTTATTATTTACTGTATTAGCCTTTTGATTATTGCCATTTGTACAAGCTGTAGCTACTGTGGTAATAGTTGCTAAACTAACTACACCTAATAATCCTGTAATTAAAAATTTCTTAGCTTTCATCTTCATAAAATGATTAGTCCTTACTCTATTTATAATAATTAAATATACAACTACTTTAAACAAAAAATAAACCATGAAAGTTTATGTTTTTTTTATAATAGCCATTAAGATTTTACTACTATAAAAAATTATTTATTGGTTTATATACCTAATTTGTTTTATCAAGAACCAATTTAATATAATTTTTAATTGCAGTGATTAATTCATCTATATTATTAAATTTTAAATTAGGAGCTAAATATTTCAAAAAAACAATCTTAATTCTTTCGTCATAAATATTTTGATTAAAGTCTAAAATATGTGCTTCAATCATTGAAAAAGAGCGAGTATTAATTGTTTTAGGGATTCCTAAATAAACTACACTATCATACTTTTTATTATTAAAAATTACTTGTGCTTTGTAAACACCTTCAGCAATATCAATTAAATTATTATCACATAAAATATTAGCAGTATTAAAACCAATATTACGAGCTATTTGATCGCCACGTACAACATTTCCCACACGATAATATGGTTTAATTAATAATTTATTAGCTATTTCAACCTGTTTATTTATAATTAATTCTTTAATTTTAGTTGTACTAACATCATTAGTAATAATTTTAATTTCAACATTAAAATATTGTTTTAGACTAGATGCATTACAAAAATTATTGCCATAAGTAAAATTAGCACCAACAATAATTTTTTTTGGAGTTAGTGGCTTTAAAATTTGATCAATAAATTCTTCCCCACTTAAATTGTTTTGATCAATATCATAAATAAAAATCCTTTTAAATCCTAAATCTTCTAATTGTTGAATTCGCTCATTAAAATCATATAATTTTTTTATTTTACGTGGGATATTTTTAAAAGTTAAAATAGTTTGGTTATTAGGATCTGATAATAAATTCATATGACCTAAATGAATTCCATCAAAAAAACCAATAATTAATTCATTAATGACGAATTGTTGCTTAATTTTATAAATATTTGTTGATGTTATTTCAATAATCATAGACTTGTTCAATTTCAATTGCATCTTTAATCTCAAAAGGTCCACTTTGCGTCCTTATTAATGTCAATAAATGAGCTAGATTATTAATTTTAGTAGCTAAATCAACAGCAAAAGAACGAACATAAAAACCTTTAGAAACATGTAAAGAAATTTTTAAAATTTGTTTTTCATAATTGAAATCCATAATTTGATATTTAAATATTTTAACTAATCGTGGTGCTATTTCAAAATCAACTTTTTGACGTGCTAAAACATAAGCTTTTTGCCCGTTGATTTTTAAAGCGCTAAATTGTGGGGGTTTTTGTCAATAATCATTTTTATAAAAATCTTTAACTATTTTAGTAACCTCAGTTAATGTAACCATTCGGTTTGTAGATGAAACAATTTCACCTGCTGCATCATAAGTATTTGTTGATGTTCCAAATTTAATTTCAGCAATATATGATTTATTATCTAGTATTAATTTTGTTAATAGTTTTGTTCCTTCATTAATTCCCAATACTAAAACACCTGAAGCATTAGGATCTAAAGTTCCAGCATGTCCAACTTTTTTAGCACCAATAACCCGTTTAACCTTTTGAACAACATCATTAGAAGTTCATTTTATTGGTTTGTTAATGATAATAATATTTTTACTAATAGCACTCATAATGTTATTATTTTATCAGTAATAAGAATAAATTATTAATAATAATTTGTTAATATTAATAAATAAAGTTTATATAGTATTAATTAAGATAAAGGAAAATATTTATTGAAAACAATTTTAATTGTAAATAAATCATTTCGTAAAAAGCGCAGTTTTGACACTCGTTTTTTGCATGATAGTGGAACGATTTATAGTAATGATCGTTTTTTAAAAATTGTGCGTGAAAGCTTTGATGCTCATATTAAGAAAAAAAGTAAAATTCTATTTTATTTCATGGAATGATGATTTCAAACTACAAATTATAAATTAAATCCTGAAATTTCACATGCAAAACGTTCTTTACGTTCATGATTAATCGAAATCTTTTTTTTATTTTGTATCGTTGCATTAATTGTATGTATTCTCGCTGGTGTTGCAAGGCCAATTATTGACTGTGTTATCAAATCAATTACATTAAGTCGTGGTCAATTTAAAGGAGATTTAATTATTGATGGACAGATTATTATGAAAAATTTATCTCCTTCTAATATTAATGGATTAACTGGTGGATCATCAATCTTAAATGTCGATCAATGAGAATTAATTCCAAAAGTTTGAAAAGCTTATTTTTCAAGTCCTAGTGTAGTTGTATTGATTTTATTAGTCCTATTTTTTTTATTTTTTAGTTGTTTATATATATACATGATTAAAAAAATTAGACCTAGAACTAATAAAATGAGCTTAGAAGATTATTTGATCGCAAGGATGAATTTAATTAATTCATTCAAATTTATACTAAAACGTAAAGTATTATTTATTGAAGGGATCAAAGAATATAACCATCGTTTTGTTTTTAATATGAATGCTGATTATAATTTAATGAGAATAATGAATTATATTTATTCTGGTTTGGCAGAATTAAACATCATTATGGTTATTGATATGGATAGTGATGAAAAAATTTTTGAATTACAAAAAGTAATTAAACAAGATTTTGATAATTTAGATTTAATCATTATAGAAAAAGAAATAGCAGAGTCACTAAAATCAAAATACCAAAATGGTTGTTTAAATAAAAAAACTAATTTAATTAACATTAATAATAAAGAAATTGATCAAAGTCAACAAACTCAAGATATTAACATTTACTAAAAAAGATGATTTCACATTTAAAATGCTATCATCTTTTTTTGTTCCATGTAAATATAAATTATTTTTATATTTAAAGTTCAAATATTAAATAAATTTAGAAAAAATTTCTAAAATTTTATATAAAAAAATAAATATTTTCTGTACATTTTTGATATTCTTAAACTGAAAAAATATATTTTAATTTAATTATACTTGCTTTATTTTTATTAAAAAAGATGGAGGTTTTATGTGTATTAGTAAAATAGATATGGCTAAGGTAAAAAAATTTTTTAAAGAATATTTATTTGCTAAATTTCAATGCAAAAATCGAGAACTATATCGTCAATTAAAAGATTATGATCCAAAAGATGATCAAAAATATTTAAAATGGGAGCATTTTGTTGAATATGTTGAGCAAGTTCTAGAAGCATTAGATAAAACTAGTGCTCAAATTATTAAAGAAATTTATATTCAAAATAAAAGAATTTGTGAATTACCTTATAGTTATTCAACATATTATGCATATCGTAAAAAAGCAATTATTGAATTACTTGCATATCTTGATTTAAAAATTTAATTAAAAAAGGCCCTAAAGGCTTTTTTTAATTAAATATAGTTAATTTTTATAATTGATAAAGATTTATTTAAATTTGCATCATTTCCATAAAATTTTGTTAATTCATCATAAATTACATCTATAAAAATTGATTCAGACAAATGGTTCATATCAATAATATTTTGTTTTTTAGCTTGTGCATCTAATCAATTATGATATTTTAAATCACTTGTAATTAACATATCTAGTTGATAACGATTAAATGCGTCATCAGCAAATTTATAACCACTACCTCCTATAATACCAATTTTTTTAAGATTTGTTTCTAGTCTAAAAACGTTGTTATATTTAATAATAGGTGATAAAAATTTTTGTTTAATAATCCGACTAATGTAATCAACACTAACACCTAATTTTAATTCTCCTACAACAAGATATGGATTCTGTTCATCTTGCTTAAGATTCAATAAGCCTAAACGTTTAGCCATTTGCATGCTCATTCCATTTGCTGATGCATCAAATGCTGTATGTAAATGAATTAAGCTGATTTTATTTTTTTTAATTTTTTCAATTAAATCGATATTAACAAAATAATTTACTTCATCATTTGAATCTTGATTTGTAAATAATGGATGATGACTAATAATTAAATTAGCATTATTTAATATTGCATCATTAACAACATCATCTGTGATATCTAATGCTATTTGTACATTATTAATTGTTTGTTGTTCATCAAAAAATAAACCATTTTTATCTCAAGCTTCAGCTTTTGATAAGTCATATTTTTTAGTTAAAAAATTCAAAATATCTTGTGCTTTAATATCCATTTTTTTCATTAATAAATTCCTTTATATATTCATATTCTTTAACTATATTTTCACTAACTAGCGAAATATCCAAAGCTTTTAAGTATTCATAACGTCTCTTTAAAAATTTAAAATATAAATTCAATGAATCCTTATGCATCTGGCTACTTATATAATAATCTTTTTCTATTAATTTTGGTACATTAATAGTTTTAGAACAAATGATTATTTCATAAAAAATTTTTTTTTCAACAAAAATGTGTTCAACTTTAATTTGATATGAGTGATTATTTAGATATTTACGTATTTTATATGATTGAGTATTTGGTTGTAAAATATAATTTTTAATTTGATTTTTATTCTGTTCTAAAATTTCTATTATACTTGTTGCTCCCATCCCTGCAATAACACAATAATCAAATGTTTGTTCCAAAATTAATCCCTTGAAACCATCATTCAAAATATTAGTAGTTCGTTTTAAAAGATTATTTTTAGTCAAATTATTGATTCCGTTTTCTAATGGACCTTGATTAACTTCTATATTAGTAACATGTTGTGCTTTATTGTTAACCAATAAGATTTTAGCTAAATATGCGTGATCACTACCAACATCGACAACATTTTGAGCGTCATTAATTAAATCAGCAATAAAAGCAATTCTAGTTTTCGGATTCACCAATAAATGAACGTAGTTTTGCGGTTTTTGATGGATGTTTTAATTTACGAATTGCTTTTGATTCAATTTGACGTGCACGTTCGCGTGTAACTCCTAAATATTCACCAACTTCTTCAAGTGTATAAGGACGTTCGAAGGGTAATAAACCATACCGCATTTTAATAACACGTTGTTCATTATCGTTTAAAACTTTTTCAAACATTTCATTAATCTGATCATTTAATGCTTTTTTTTCAGTATATTTTTCTGGAGAAATAATTTCATCATCAGAAATGAAATCTGAAAATTGTGATTCTTCATCATGACCAATTTGTTTATCTAATGAAACTGGGTCAACATTTAATTTTTTAATCTCAACAACTTTTTGTGCAGTGATTAACTGTTCTTTTTGATTTTTTGCTTGTGAAACTTTGTTCATAGCTTGCGCAATTTCTTCAGCCGTTGGATCCCTTCCTAATTCTTGAATTAAATTTCGCTCGGCTTTAGTTAATTTGTTAATTGTTTCAACCATATGAACAGGAATTCGAATTTGTCGAGCTTGATCAGCAATTGCGCGCGTAATTGATTGGCGAATTCATCATGTTGCATATGTACTAAATTTATGTCCTTTTTCATGATCAAATTTATCAATTGCTTTTAATAAACCGGATGACCCTTCTTGAATTAAATCTTCTAAGTCTAATCCACGATTTAAATATTTTTTAGCAATAGAAGTAACTAAACGCATATTTGATGTATAAAGTTGATTTTTACCAATTTTAACTAGAGATGGATCGTCACTAATAACCATTTTAGCATATTCAGTTTCCTCATCAGAGCGTAACATTTTTGATTCTCCAAGTACTCCTAAATAAGCTTTAATACCATCATCAACTTTTTCGGTTGTTGTTGATGAAAAACCAATTTTTGATGTGCTAATTTCTAAAGTATCATCAATACCATATTCATCCTTTTTTTTGGTGTTTGTCTTTGATTTTGATTGCATCACCAAATCATAATCAGGCACACGAATTCCAATAGCTTCTAGAACATCAATAATTTCACTAAAGTGTTCTTCACGTAAATTCATATGAATAAAATAATTTTGAAGTTTATTAAATTTAATTTCATTTCGAGAACGTTTTTTCTTACTAACATCTAAAACCACATTTAATAAAATTTCTTCAGGATCACCAGCATCTGCACCTTTTAAATTACGACTTTCAAAAATTCCTGCTTCAAGTTCAGAAAGTGCATACGAAAATGAGAATTCTTCAGTAAATGATAATTTAACTTTTTGTTTTAAATCCTCTAGATTTACAAATAATGGTTCATTTTGTTTTGTTGACATCTAAATATCCTCATCGCTCATATTTTAATTTTAATTATAACATAATTAAAAAATAAACACTTCAGTGTTTATTTTTTAATATTTCTTATTTTTCTCCAAAAACAATATTATAAACTTCATCATAGTGTTTTACCAAATGAATTTTAATATCATCTAATATATACTTTGGAACATCTTCTAAGTAACGTTCGTCATCAATTGGTAAAATAATTTCACGAACTCCAGCACGATATGCTGAAATAACTTTTTCTTTAACACCACCAATAATTCCAACATTACCACGTAGCATAATTTCTCCTGTCATTGATAGTGTTGTACGCACTGGTCTTTGACTTAATGAACTAATAATTGCTGTTGTAATCGCAATTCCAGCAGACGGTCCATCTTTAGGAATTCCTCCGCTTGGAACATGAACATGAATATCAATTTCTTTAAAAGAAACTTTTTTAGTATCAATGCCATATTTATCTGCATTTGCTTTAACAAAACCTAAAGCAACAGATACAGATTCATTCATTGTTTTTTCAAGATTTCCAGTAATAGTTACATTTCCACCTTTTCCATTGGTTGAATGATTAACTTCAATAGGCAATAAATCGCCACCAGCCGCTGTATAAGCCATTCCATTAACAATTCCTGGAATTGTTGTCTCTTCTTTTTTATTAAAATCAAACTTAATTTTACCTAAATATTTAATAATCATGGCTTCATCAACTTTTAAATTCAAAGGTGATTGATTATTTTTATTCTTATAAGTTTCAACAATGTACTTGCGTACAATATGACCTAATTGACGATCTAATTCTCGTACTCCAGCTTCTTTAGTATAATGATTAATAATAAAATCTAAAGCATCATCAGTAAAAATAAGTTCATTTTCATTTAATTTAGCATCCATAAAAATTCTTTTTAATAGATGTGATTTTGCTATTTCACGTTTTTCAATTGCTGTATAACTCGATAGTTCAATTACTTCTAAACGATCAATCAAAGCATAAGGAATTTGTTGATAATAATTAGCTGTTGCCATAAACATCACTTTGCTCAAATCATATTCTTCTTCAATGTAATTATCTGAAAAATTTTTATTTTGTTCAGGATCTAATACTTCAAGCATTGCGGCAGCAGGATCTCCTCGTTGATCAGATGTCATTTTATCAATTTCATCTAATAGGAATAAAGGATTGATAACGCCAGCCTTTTTCATACCCTTAATAATACGTCCGGGCATAGCTCCAACATAAGTTTTACGATGACCTCGAATTTCAGATTCATCTCGCACACCACCTAAACTAACTTTAACAAATTTTTTATTTAAAGCTTGTGCAATCGAAGTAACAAGTGATGTTTTACCAACACCTGGAGGGCCAACTAAACAAATGATTGGACCTTTAAGATTAGGGTTACGCATACGTAATGCTAAATACTCTAAAATTCTTTCTTTTACCTTTTCAATACCATAGTGATTATCATCTAAATGAGTTCTTACAGACATCAAATCAGTATTATCAACACCCTGTTGAGTTCATGGTAAATCCAATAATCAATCAAGATATGATTTAATAACTGATGATTCATTTGATGACATAGCATTCTCAAAACGATTTAATTCAGCTAAAGCACGTTCTTTAACATTTGGTGGTAATTCTAAATCTTCAATTTTTGCACGAATTTTATCAGCGTCTTCTTCACGACTACTTAGTTCACCTAATTGTTCTTTAACAACTTTTAATTTTTCGCGTAAATAGAACTCACGTTGTTGTTTTTGAAAATTATTATTAATTTTATCAGTAATAGTTTTTTCAACTTCATCTTCAATAACGCGCTTATCTACTAATTGATCAAATAATAAATGTAAAATTGTCTCATGAACTAATAATAAATTAGGTTGCAATAAGATATTAATTCGATCTTCAATAGTTTTTTCACTAATTTCATTATCATAATCATTAATATAAGTTGCCGCAGCTAAATAAGCAAACTTTTCTTTTTCAAGTGAAATTAAAGCGTGCATATTATCAAAATTGATATTTTTATGTCTTGAACGCTTGTTAATTATATTGAAAATTTCAGAATTAATACCATTGATTGCTATTTCATCTTTATTTGATAAAATAGGGTTTGTAATGACATCTTCATATTCATATTCAATAGCATCAATAACATCATTCTCATTATTAATGTAAACAGCTTTTAATCCTTCAACTTCAATTGAATAACCTTCATCAAAATTATCAATAATAGATTTAATTTTAACTAACGTTCCTACTTTATACAATTCATCAAAATTAACAACTTCATCAGTATCAATATCTTTTTGTGAAATAATAATAATTTCTTTACTTGAAGATTGTTTAGCTAAATCAATCGCTTGGATTGATTTAGGGCGTCCTACTTCAATTGTTGTTGTTTCATAAGGTAAAACAACAATTGCTCGTGATATTAAGATAGGTTTTTTCATTAACAACCTCTTTTCTAATCTTTATTGTATTAAGATATTATAACAAAAACAAATAAAAATTAGCAAACTATTATTACGAGTGCTAATTTTATACATTGATTTTGCCTTTTTGTCCTAAATTTTCTTTAATTCTTATTTGATTGTCTTTATTTTGAAGATAAAGTTGTATTTTTTGGCTGATTATTTTTGTTTAAGTCAATTAAATAATTAACAACTTTTTTATGTAACAAGAACTCTTTAACAGCATCAGGATTTTTTGATAATTGTTCAATAACAGCTTGTTCTTCCCCTTGGTATAGTTTAGCCATTTCCGCAACACGTTCTTTAACTTCTTCATCAGATACAACAATATTTTGTGTTTCAGCAATATCATCAACTAACAGTGCTAAAGTTATAGTTTCTTTAGCTTGTCTTATCAAGTCTTCTTGAAATTGTGCTTCTGATTTTTTACTAAATTCCAAATATTGTTTTAATTCAATTTTATATTGTGATAATTGTGATAAAACTTGTTGTCTTAATGTTGAAACTTCACGATCAATTAATGATTGAGGAATTGGTGTTATCTTTGCTTTAGCTAATAATTGTTGAGCAATCTCTAATCAGGCACGTTCACTATTCTTTTCTTGTTTAGCTAAAACAATTTGATTGTGAATATAATCTTTTAATTCTTGAACTGTATTAACATTAGGCAAATTAAATGATTTAGCAAATTCATCATTTAATTCTTGTGGCTTTACATTTGTAATTTCTTTGATAAAAACTTCAAATACTGCTGCTTTGCCTGCTAAATCGGTTGCATGATAATCATCTGGAAATTTAACATTAATATTTTTACGTTCATCTTTTTTTAAACCTATCATTTGATCTTCAAATCCTGGAATAAATTGATTAGATCCAATTGTTAATAAATGATCTTTAGCACTTCCACCTTCAAAAGGCACACTATCAATCATCCCTTTAAAATCAAATCGAACTTCATCCCCTTTTTCAATGAATTCATTATCATCTTTAACACGTCTTTGTGATTTAGAATTTAATGCATGTTCAATTTCAGCATTAACTTCATGTTCAAAAGCTTCATTCACAATTGGTGTTAAAATTAAATCGTTATAACTTTCAATTGTCGCAACAGGGTATAAATCATAAACAACTTTTAAACTTAGTTCTCCATCTTTAAAATCTAATATATCAACACTTGGAGTGTCAACAGTTTCTGAATCATCATTTATAAATGATTCATCTTGTTCTAAAAAAGTTAAAACTTGCGGTATTAAATCATTTGTTGTTGTTTCAAAAACTTCAATTTCATTAACGTGTTTTTTAGCTTCTTCAACCGGAACTTTTCCTGGACGAAAACCAGGAATTTTTAATTTTGAAGCTGTTTTTTTTGCAATTTTTTCAAATGATTCTTTGTGTTTTTTCTCTCATTCTGATTTTTCAATTAAAATTTGAAATTCAATCGCATTTTCGTTCTTAATTTTATTTAGTAATTTCATATATATTCCACCAATTAACGTTTATTACATCATGTATATTAATATAAAGTTTACATAATATTTTTATATTTTAACATTTATAAAGATTTTTTTATTAGTTAGTTGATTCATCAATAGGACTAAATTCATTTATTTCTTTATTTAATCTTGGTAATTCTGTTAAATTTCTTATACCAAATAAATCATAAAATTTTTGACTAACAGTATATAAAATTGGGTGACCTGGTGTGTCTTGACGGCCTGCTTCTATAATCAAATTCAATTTTAAAAGAGCGTCAATGGCAGGTTTGGGATCACGATCACGAATTGCTTGAATTTTTGCACGAGTTGTTGGTTGATTATAAGCAATAATAGCAAGCGTTTCTAAATTTCTTTGACTTAAGGGAGTTCTAAAACTTGTTGTAACAAATTTTGATAAAGCATCTTTATTTTTGGATTGAGTTAATAATTTAAATTTTTCACCAAATTGTACTAAAACTAAACCCGAATCATCAACGTCATAGTCTTTTTGTAATTGATTTAAAATTGTTTTAAGTTGAGAACTCTCATTTAATCCTGTTGTTCTTTTTAAATCTTGCAAAGAAACACCTTCTTCACCAGCTAAAAACAGTGTAGAATCAATAATTGATTTAATGTTTGTAATTTGATTTTGATTCAAATCATTTATATTAAATGAATTTCGAAAAGAAACACTACTATTTTTTTTAACATCCTTAATTTTAATTTCTTCAGTAACATTAATATCTTTTGTTTTAAATATCTTGTTAAAATCTTCTTGTGGTTTTAATTTTTCACGTAATAATTCATGATTGGTTAATTGATTCTGCAAGAATTTAGAATTTTGCTTAAAATTATTATTTTTTTGTACTAATTTAGAATTAACAAACTCTAAGAAATCTTGACCTGATAATGCTTTTGGTTGATTAATAATTTCGTTGCCTGATTCAATTTGTTCATGATTATTTAACTTATCATCTTTTTCTATAAATAAACGAGTTTTATGATTAATGTGTTTTTTTTGATAAATTTGTTGATTGTTATCAATAATTTCATCATCAATACCATCTCAATCTAATTCTAATTCATTTTCATCATCATTGTTATCTGCGGATAGATTATGTAATGTATATTTAGCTAAATCTAATTCATTATTTAATAAATTCTTTTTATTTTTTTTATCATTATTCATTGTCTTCACCTTTAAAATTAATAATTGTATCTTTTGTTAAATTACTTGTTAAATTTTTAGCTTGTTCTACACTACTAGAATTATAATCATCAACAACTCTTTCAATATTAATTTTTAATAAATAATCATTATCTAATTCTTCAAATAAAATTTCATTACGATTAATTAAAATTAAAATAATTAAAAACATTATTGCAAAAAAACGTTTATTTTTATCAATCACTGCTTGTATATCAAAAAAACTAAGCATACTTGCTTGTTGATTAGGCGATTGTTTTAAATAATTTAATAACAATTGTTCTAATTGTTTAACATCATATTCATGAACTTTAATCTTATTAATTTTATAATTTTTTAATTCATTTTCATAAATGATTTTATCCATAATATTTTTTAAGATCATGGAGTCTAAATGATTTGGCAAAGGCTTATAAATTGCATCTTTTAAATAAAATTGCTCATAATCTTGTTGTGGTAAATCCATCATAGTTGCTCTAAATTCAAAACGTTGTTTTAAATGTTCGCTTAAATTTTTATAACCATTGTATTCAATTAAACGATCAATTAAATTTTGGCGATCTTCTTCAATACTCATAATTTGTTTTTCATCAACAGTTGGCATCAACATTCGAGTCTTAAGTTCTAGTAAATAAGAAGCCATTGCTAAATGGTCACCTAAAATATCAATATCGATCGTTTTAATTAATTGATTTACAAAATTAACATATTGTTTTGACAACTCTAAAATATCTAAATTATTAATATCTAAACGTTTTTGTTTAATTAAAACACACAATGTATCTAATGGACCGTTAAAATCAATAAACTTATATTCAAATAAACTATTTTGGATTTGCTTTTCACTCATTCTATTCACCAAAAATTATTATATTAAACTTATAAATTAATTATAAAAAAAATTAGTTTTAAAAATACTTTTAGTATAAAAAAACAATAAATTCAATAAACTTTTTTTAATTTATTTAAAGTATTTACTGTTAATGAAAATTAATTTGTTTTAATTGTTGATTTTAACAATTCATCATAATTTTTTTTAATGTTTGATTGAATCTTACGCATTAAAATATCTTTATTAATATTAATGTAATTAAAAGAATGAATTGGTTCTAAAAAACTAACTTTAATATGTTGGGTTTTAAAAGGATTTGATCTTAACTTTTGCTTTTGATCGTTTTTCATTTCAAACGAACCTTGTGAGTTAAAAATCACAAAAGGTTGAATCGAAGATAATGTATTATAAGCAGCTGTTAAAGCGCCTGATTTAAACTCATTAAATTCATGATTAAAATTTCGTGTTCCTTCTGGAAAAATAACTAATCCACGATAATCTTGTTTTAAAGCATTAACTTGTTCATTAATTGTTGAAATTGATTGTCTTAAATTATTACGGTCAATGTAAATTACATCAATTAAATTAAATAAAAATTTTAAAAATGATTTTTTTAATTCAATTTTAGCAACAAAACGCACATTATGAGGGCTAATTTGATAAAGAGCCTTTAATAAAACAATTGGATCACCATTAGATTTGTGATTACCTATGTATAACATGCTTTTTATACTAACATTTTCTTTACCAATCAAATCTACTTTAATATTTTTTAAATATAAAATAGCATCAATAATACCCATTATTTTTTTATAGCGCTCATTAACGCTAACAGACTCTGGATCATTTTTATATTTATTTGCCATCATAGCCGCTAAAATCATTTGAATTATTATGTATAATGCTACAAAAGGATATGCAACAAATCACAGTAAGGTTTTTAAAAAATTCATTATACCACCATTAATTATTATAAATATGATTAACTAATTATTTGAAAATAATTAGTTAATACTTAACTCTAGTTTATTAATATCTTTAAGCAATAATTGTTTGTTATTTTTATTATTTATAATTAAACTTGCTTTTACAATAATTAAATCTCCATCATTAAGATTTTTTAGATCAATATGATCATTATTATATAAAGTTATGATTTTGGCTTCATCATTAATAGGATCATCGCCTAAATAAGTTAGTAATTCGATAATTTCTTCATTGTGTTTTTTTAAAATAATGTATCCACTAAAAACATAATCATTTTCATTAATTAAATTAATATCAAAATTCATAAATATATACACCTCATTAATAAATTATGAGATTTATAGAAAAATGAAAGTTTTAATTTTGTTTGTTATTTTTTTATTAAAAATTTAGCTTTTTTATTTTTTTTAAATAAATTTCATACAAATGTTGTACACATTCATCTAATTTCATGTTATCGTTAAAAATTTCAACAGCATCGTTTGCTTTTTTAAGTGGAGCAATTTTGCGAGTCATATCACGATGATCACGTTCTTTAATATCGTTATATATTGATTCATAATCCAAATTTTCAATATCTTTATTCTGAAACAAACGTCGTTTAGCGCGTGTTTGAATGCTCGTATTTAAATAAAATTTAAGATCAGCATTAGTTAAAACAATACTTCCAATATCGCGCCCATCCATAACAACGTTGTTTTCACTAGCAATTCTGCGTTGTTCATTAGTTGCTAAATCTCGAATTTTTTCATTAGCAGATACAACACTAGCAATCATTGCCACATCATTACTTCTTAAGATAAGACTAACATCGTTGTCATTAATTTTCACATTATCACCATTAAAAATAAAATTAGCATTTTTAATGGCATTAATACAATCCATTTCGTTAACATTAATATCTAATTTATTAATAGATAAAAAATAAGCATAAGCACGATACATTAAACCAGTGTTAATATATAAAAATCCCAATTTCTTGGCTAATTCTTTTGCTGCTGTACTTTTTCCAGAACCTGAAGGTCCATCAATAGCGATACTGATATATTTTTTCATTAATTAATAACTCCTGCAATTAATAATGCACATATAATTATGACGATTAATAAACATAATAAACAAGAAGTTAAAACTATAAAAATGATGGTTTTGATGTTTAATTTTGAATCAAATGGCATTTGATGATTTAAAGTATGAATTTTAGGTTCATCCATTGTAATTTTAAAATGTTCATATGCATCATAATTTTTTAATTGATAATCTTCAAATTCACTACTAATTAAACTTAATTTTTTATTATTTTTAATATAAAACTCACTATCTTTAGTTTGAATTTGAATATTAACTTGATTTAATTCAAGAATTATTTCACTAATTTGTCTTACATCATAAACACTAACCAGATCTTGAACTTGTGAAATAACATATGGTGTCATATTTTTATAATCAAATTTAAATTCATCAAATAAATTTTGATTTTTAATCTCACTAAAGTTAATTCAATCAAGTTTTTTGATTTTATCTTCAATCTCTTTTAAGCTTTGTTTTTGGACTCTAATTAAAGTATAAAACTTATTTAACTCATTAATTGTTAATCTAGTTTTAACATGAATTTGTTTACGATCTATCATTAAATAATTTAATATTTCATTATTCTTTTAAAAATATTACTTAAATAACTTCGCTTTATAAGAAATAACACTACCGCTAATCATTTCAAATAAATCACTAATTGAATTTGCAACACTATCAATCTTATTTAAATACTTAATTTTTTTATTTAATCCAAACACATGAATTGTGAATAGTCCACTTTCAAAATTAGCATCTTTATCTTCTAAAATCAATAATTCATTACCTAAATGATCATATTTTGAAGGACTAATTTCGCTAAAGGCATGATTTAATTTGTAGGAATTATTGAACTTAATTCGTTTATTAGTTTTTTTCTCGATAACGTGTGCATAATTAACAACTGGACGACCAAATTTTTTAAATAGATGATTTGAAGTAATTATAACTGCATACGATAAAGCATCACTAGAATCAATAATTTTAATTTTTGCTTGTTCTAGATCTTTAAAATTATCTAGTTGATTTTCACTATAAATATTCTTATTAATATCTGTAACAATTACATCTTCTATTACATGATATTCATTTTCACAATTAGCAAACAAATAAGGATGTTTATGCTCTAATGAACCAATAATTAATTTATCATCTTCAAAACTAATCTGTGGAGCACAAACATAAGTAGAACCTGTGGCAATAATTTTTGCTTCATTTAATTTCGCTTCAAAATCGTCTAAATATAAAATATTGTTTCGGTTTGTTGATAGCTTATGATGCACTGCATAAACTTTAATTTCATATTTATGTGATTTATTTGGCGGGCAAGGAGGGAAATAATTATTTGCTATATCAGTTTGATAATTTGCATCTAATATTGAATGTGAAAAATGTTTTGAAGTTGAATTTTCACCTTGATATAAATTAGCATAACCAAGATTTGCAACATTTAATGCTAATTCGGGTTCAAAAATGTTTAAGGCATATCAATGCACAAATGGAGCGCCTCCTACTGCTTCATAATCAATAATTGCAACCGCATAACTTGCTGTTTCAAGTACTGGTGATCAACGAATTTGAGGTGAAATATTATTTAAACCATAATTTTGTTCGTCAAAATTATTAAATTTATCCAAACCTTTAATTTCAATTTTAATTTGCTCTCCCTCTTTAATTTGCTTTAAAAATTTATTTTCTACCATATACTCAAATTTTCCTTTATTTTTAATATATACATAAATTAAATATTAACTTATACATTATTATATAACAATAACCAATGGTATTTAAACCATTGGTTATTAAAATTAATTATTTCTTTGTTGAATCATCAGTTACAGTTGCTTCAGTAACATCATCTTTATTATTGTTTTGATTATCTGGATTTTGCTGTTGAGCTTGTTGAGCAAATTTAGCAGCATTAGTTAATAAATCTTCAACTTTTTTAACTTCAGCTTCTAGTTTTACATAATCTTGAGCATCGCGTGCGTTTGTTAAATCTTCAATTTGTTTGTTAAATACATCTTTTTGTTCTTGTGGAACATTTTCAGTTTTTGACACTTGTTCTAATTGGTTAATTAGACTATCAGCACGATTTACAAGTTCACGTTCATGTTTTACTTTAGCATCTTTTTCTTTGTTTTCCTCAGCTTCCTTAATCATTTTGTCAATTTCATCTTGACTTAATCCTTGTGAATCTTTAATTGTGATTGATGTTTCTTTTTGTGTTTTTAAATCTTTAGCGTTAACACTCATGATCCCATTAGCATCAATATTAAACTTAATTTCAATTTGCGGAACACCACGTGGAGCTGGTTCAATTCCGCTTAATTCAAAATTACCTAATAATTTATTATCAGCAGCTAATGAACGTTCACCTTGCACAATTCTTATATCAACACTTGGTTGATTATCAGCAGCTGTTGAAAAAATTTGTGATTTACTTACTGGAATCTTTGTGTTTCTTGGAATTAAAGGAGTTGCTACACCTCCCATTGTTTCAATTGATAAAGTAAGTGGTGTTACATCAAGTAATAGAATATCATCAATTTCTCCTGCTAATACTCCACCTTGAATAGCTGCACCAATTGCAACAACTTCATCAGGATTTAGTGAATGGTTTGGTTCTTTACCTGTTAATTCTTTCACTAAATTTTGTACTGCAGGCATACGTGTTGAACCACCTACCATCAAAACTTGATCAATATCAACCAATGATAATTTAGATTCTTTCATTGCATCTAAAACAGGTTTTTTTAATCTTTCAATTAAATTTTTAGTTAGATTATCAAATGTCGCTCGATTTAATTCTTTTTCAAAATTAATTGGCTGACCAGAACTATCCATTGCAATAAAAGGTAATGAAATAGTTGTTGTGTTTATACCTGATAATTCAATTTTAGCTTTTTCAGCAGCATCTTTTAAACGTTGCATAGCCATTTTGTTTTTACTTAAATCAATATTGAATTCATCAGCAATAGATTTTAATAATCAATTAATAATAACTTGATCTCAATCATCACCACCTAAATGGTTATCACCACTTGTTGATAATACTTCAAATGTACCATCTGCCATATCTAAAACTGATACATCAAATGTACCACCACCTAAATCAAATACCAATATTTTTTGTTCTTTATCTAATTTATCAATTCCATAAGCTAAAGCTGCAGCTGTTGGTTCATTAATAATTCTTTCAACGCTCAATCCTGCAATAATTCCTGCATTTTTTGTAGCTTGACGTTGTGCATCATCAAAATATGCTGGAACTGTAATTACAGCCTTATTAACTTTTGCTCCAATTTTCTTTTCTGCATACTCTTTAATATAAGTTAAAATTTTTGCTGAAATCTCTTCTGGAGTATAATCTTTATTTAAAACAGTTACTTTTTCTTTTGTACCCATTAAACGTTTGATTGATGAAATAGTATTTGGATTAGTTATTTGTTTACGTTTTGCAGCATCACCTACTAAAACTTCATCGCCGTTAAATGAAACTACAGATGGAACTGTTCGTTTTCCTTCTGGATTCTCTAAAACAATTGGTTTTTTATTTTCAATTACGGCTACACATGAATTTGTTGTACCCAAATCAATTCCTAAAATAATTTCTTTTGCCATAAATATCACATTCTCCTTTTAAACATATACCATATTTGAAAGTATAAGATATTATACATTATTTTTTGGCACTTGTCAATATTGAGTGCTAATTTTATAAAAATAAATTTTCGTTTGTTAAAAATAATTTGATTGAGTTTAATTTTTAATGCAAATTATTTTTTATTTTTGTTTTCTATAAGCTTACTTTTCTTAATCAAGTTGATAATTCTTGTTCAGTTTCTTCGTCTAATTCTATTTTATTACTTATCGTAATGAAATAACCATCATTGTCTTTTAAGAAGCTAAATTCATTTTTATTTAAATTCGCATTTGTGACGATTGTATCTAATTCTTTTAAATAAATTTTAAAATCATTATCGCTATTATTTTTGGATTCATTATCATTAAAATCAAGAATTTCATCAATAGAACAAGCACTATGATCTTCAAAATTTGTTAATAAAATATTATTAAATGGGCGTGTAAAATATTTTAAACCTATTTCTTCTGTTAACTGTTTATATTTTAAAACATATAAACAAATAAATGGATATTCATATTCGATGTACTCTTTGTATTGTACAGGAACAATGTGTGTTCTGCCATGCGCATATACTGGTACATTATCTACACGATTATAAACATGATAACTTTTAATATTAAACTGTGCTTTATTAATGCCGAATCGTGTTTGATTATTAACTAGTTCAGCAAAAGGAATTACTTCACAATCATTAACAAAAGCTATTTGGTTTTTTAAAAAAAGTTTTGTCGAAACTGATAGTGGAGTTAATTGATTTTGATTAGTATAATAATAACTTTCATAATCGTAATTATCACCAATTTTATAAGTATCATTATTAATATATCATTCTCGGGCAATTACACTTGATAAACTTGTTGTAGTTAAATATAAAATATATTTTTCAAATAATTTTACTAATTCATATTTTAAAATATTTAAATTCGTTTGTAGGTCCGCATTAATATCTGTATCAATTAGAAAATCACAAGTTTTTACATAACTATTATTTGTTAATAAATTTATAAAGAATGGTTTTCTATTATCTTTTAATAATTGATCATCAATAATAAGGGATGTCGCAGTTTTTGTAAACATAGGAACTGTGATATCATCTTGTTTTAAATATTTAGCAAAATTTTCATAGTTTTCTTGACTAAAAGGCGTAAAAAATTCTAGCATTTTTGTATCAAATTCTAAATTTTGAGTAGCAATTCCTAAGCCATAATACTTAGAAAATTCTAAATTAGAAAAAATTGTTTTTGAACGAGAATTAACAGTTTGAGCGTACGTTGAAAAGTTAAAGTTTGGAGAGTAATTAGTTTTATGAACAAAATTACTGTGAAGATCAACAAACGGTGTTGGTTCCTTATGATGAGCAACGATCGTTTCACTAACCATTTGATCTCCTTTTCGATAAGAAATTTGAATACAACCACTTGTTACAACATCTTTAATTGAAAATTCCTTATAATACACTAAAAAAGTATCCGTACTTTTAAATTTAACTTCTAAACCACCAAAAAATCCAACAAATTTTGCGTTTCGTGAAATTAATAAATCGGTTTGTTTAATATTTTCATATTGAATTTCTGGCATTAAATATAAACCATTCTTAGCAAAAATTTCTTGCGAAATTTTATAAGAATTAATTTGTGATAAAACATTATAAATATATTTATTTTTTTGGCTGTTACATATATTAATTTGATTATTTTTTTCAATTTCATACTTTTTAAAATTTTTAATAAATTTGGAATTTTTCAAATAAAAATGTAAAAAAAACAAACCAATAATAATAACAAAACAAACTGCGATTAGAATATTACAAAAAGTCCGTTGTTTAATTTTTTCATCTTTTTTGCGTTGTTTAATATCATTTTTTAAATTATTAATATCGGTTACTAAATGATTAATTTTATCAATATTATTATCTTGATCTTTTAATTGTTCTAAAATTACATGATCAAGATCGCTGTTTAAATTTTTTTTAAATTCATCAAAAAATCCATAAACAGGATTTTGGATTAGTTGTTTAATTTTTTGCTTGTCAATCATAATAAATACTATTTTCTTATTTGATTATTTATATTATTATATAATACTACAAAAAAATATGGCTGATAAGTAACACTTATTTAAAATAATTCAAAAATAAAAATAACGTCGTTAATTTACCGATTCCACCTGGTACAGGAGTGTATTTAATGTTTCGATTTAATGCATCATTTTTATTAATTAAAAAAGAACCACATAACTTATTATTGATGTATTCTGTAGTAACATCAATTAAATAAGAATTATCACTTAATGCATTAGAATTAAAAATGTGTGCTTTATTCACACAGCTAATAATAAAATTAGATTCTTTAATAATTCTTAATGGATTTTTAGTTTGTGAATGAATAATATTAATTTTATTATTTTTAGGATTTAATAACTTATATAAATAATCCCCTAAATAGGGTGAACGACCAATAATGCAAATTCGATAGTCATTTAAATTTACATCTAATTCTTTAATTAATGTTAAAATTGCATTAATTGTTGCTGGATGGTAAATAATTTGATTTTCTTGTTTTTTATTTAAAAACAAATGTGAATTAATTCCATCGAGATCATTTTGAAAAGTTAAATATTGAAAATAATTTTGATCCAAATGTTTTTTTGACAAAGGGAATTCAAAAAACAAAAAATCCTTATTTGCTTGTTTAAGTTTTGATAAAAAATGATGATTTTCTTGAATTTGGTATGCATTTAAATCATAAATAATAAGTTTAACAGAAAATAATTTAGCATATTTTAATTTTGATTGAATATATGCACTATTAAAATTTACTAAATCTGAATCATAAAAAACATAAATACGTTTTTTAGTTTTGTTTTTTTTAGTTAATAATTCTGTTAAGACTTTTTCGTATAAAAAATCAATTTTTAGTTTCATTTTGAAATAAAAAGGCTTTTATTATTAAAAGCCTTTTTTGTGTTTCCTTTAAAATAATTATTTAGAAAGAATTTCTTGATCGTTAACGTAAACCTTAACACCATCAACTTTATCATCTTTTGAAATTAATTCTAATTCATATTTTGGCTCAATAATCGTTTTAATAACTTTTGTTTTATTTAAAACATTAGAATTATGTTTAATCTCATTTATTTTTGTGATATTACTTTCTAAATCATCTAAAATAACATCAGCATTAAAATCATAATTATCATATTTTAAAGTTTCAATTTTTGCTAACAATTGTGTTTGTTTTTCAATTCGGTTTAAAATATCAAAATAAACAGCATAATTTGAAAGAGGGTGGTTTAATGTTTCTAATCTTAAACGTAAACTATTTAATTTTTTATAATGCTCAATTAAAAATTTTAAAATAACAATTTCTGAACCTAAACCATACATAAATTTAACACGTAAATCATACAAAGATTTCAAATATTTTTTTTCATCTTTAAAGTGACCATTAATATGATGTTGATTTAAAATAGCACGTTTTGTATAATTTCGAGTCCGAATTAATTCTTGATGGTTTAATAGATCTTCTAAATCATTAATGATTCCGATCTCGTTAGAAATAAATAAAGTTATTTTTTTAAAATTGTTTCAATTATCGCCTGAGCTTAACTCAAATAATTTATCATCTTTTAATAATTCATCATATTCTTTCTTGAGAATATCACTACGTTTATTTAAATAATTTTGTGTAATTTGTGAACGATGTTTTTCAAATTCAGTAAGAATTTTATTTTTGTTTTTAACTTGTCTTTTTGTTAAAAAACTATCTTCATTGATTAATGATTTTACTTTCAATTTATGTTCTTGTTTTTGATCGATTTCTATTAATTGTTGTTGAAATTTAAAATTAACTTCCCCTAAAATTTCAATTTTTCATTCATCAATTAGCTGTTCACAAATTTTTTCGACTTCTTTTAAATGAGAATTTAATAATTCTTCATTTTTCATAATTATATTATTCGCACTTGTATTCTCATTTTTTTGATAATCTTGAATTAGTTGGTTAGAAACATTATTGTATAAATCATCTACTTTACTTTCTAATGTTTTAGTATACTCTTCAATTTTGCTAATATCAACACTATTATTTAAAATAGTTAATTCTTTTATTTTTTCTTGTTCACAACCATTACCTAACAAAGGATCATAAACATTACTTAAGCTAATAGTAGAATCTAAAATATCTTTGAATTCTTGTTCATTTTTAACAAAAACTAGATTATCGCGTCTTTGAATTGAATTATTTTGTTTATTGGTTAATAAATCCACAATTTGATTAGTTTTATTCATAATTAATAATTCCTTTTTTTTTATTTATCTTTTTTTTGAGTTAAATCATGTCCAAAATTTCTTCTTTTCTTTAGGTTTATTATTATTTAATTCATCCAATAAACGTTCAGTTTCGTCATTAATTTCTTGTGTCTTAAGTTCAAAATCAGCAGGTGTTAATTCAATTTGTGATGATAAATCATTATTTTCTATTTCTTTTGGTGTTAAGAAAGTATTTTCTTTAGCTTTTTCTTCTTTAAAATTATCAATACTATTACTATTTAAAACATCTTGGTTAACTGATTCTTTTTGGTTTTCAGGTATTTCAAATTCAAAATTAACCATTTCCTCTTCAGGTTCATATTTTTTTTCAACACTCATAACACCAACATCAGCTTTATTATTTAAATTTTTGGTATTAATTTCTTCTTCAATAATTACATTTTGATTATCTGATGATTCTAATTCTTGATCTTGAATTCGTATTTGTTCATTTAAATTATTTAAACTATCATCAATATTTTTTTCATTACTATCTATCATAATTGAATTTAGTTCGGTTTGTAATTTAGTATCTTTTTCATGTACATTAATAATTTCATCTAAACGAATTTGAATTGATGAAAGATCATTTTTAATAATATTTTCTTGAGATGATTGCTCTTGTTTAAGATCGTTAAAACTAGTTATTTGCTTAGTTTTAATTAAATTATCAACATTTTCATCCGTTTTTACTACTTGAGTAACTTCAGATGAATCATTAAATTCAACAGCACTTGTAGAATCTTGATTTTCTAATTTATCAGTATTGTCCAATAATTCTTCATTTGCTTCTTGATCATCATTTTTAACTTTAAAATCATGATCATCTTGTTCGACAAGCTTTTGTTGTTCTAAAATGGTATTTTTTAATATTTCTTGTTTAATTGCATATGGATCATCATTTTTAATAATTTCATATCCTGCCATTAAAAAGTCTACCCGGTTCTCAATGTCAACCATAAATGGAGTTTCATGATGACGTAAGTAACTACGTGGCTTCAAATACCCATTTTTTTTATCGATTACTAATTGTTCTTTGTTTAATTCTTGTTTATTTTCGCTAACTTCGTCAAAACAGTTAATTCTTCCAATACTATAGAACTCTTTTTCTAAATTAGATTGTTGTTCATCACTTAATTGTGAATTTTCAAATTTTTTTAATTCAGCTATTTTAATTGATTCATTATTTTTTTCTAGTTCTTGTTTTGGTTTCTTATTGTGCAAAAAATCATATTCAAGATCGTCAAAATCAATTGAGGTATCAACTAATTGACGTGAGTTTCTAATGAAATTACGTCTTGATTCATAATCATAACGTTGAAATGAAAACGGCAAGTATTCTAAATCATTAACTAATAATTGACGAATACTTATTTTATTTTGATCATCTTTAAAAAGATCGTCAAAATCTAACTCAAGTAATGAAGATCTTAAATAAATTAAAATTTGAAGAATATCTAAATTTGATTCAAGGGTTGCTTTTTTATCATAAAATTTCTCATAATCTAATTTATTTTGACCATGAATACTTAAATTATGAATTTGTTTACTAATTCGTGCATTATTAAAATGTAATTCTTGTATTCATAAATCATATTTTGTTTTTAGATTAACGATTTCTTTGCTCGTTAATTGTTTTCTTTTAAAATCATCACCAATAAATGCATCTAAACCATCATTAGTTCAAAATTCTTTATTAATTAAATATTGACGAGTTTTTGTGTAAACTAATTGATCATACTCATCTAAATTAGTAGCTGCTATTTTTGCAAATTGATAGTTATCTAAAACATCAAGCTCCATAGTCTTAAATTGTTCTAAACTATTAAAATAAGCATAACCTTTACTAATACTTTTTTTTAAGTAAAACTCATCTTTTATACTATTAATTAAATGAACTTTTAACTTTTGATCATCTAAAAAAATCTCTTGTGTTAAAGGGTTATTAATCCCTAGTTTTTGAGCTTGTTCTACATTCAAAAACAAAGTCTTGTTGTTTTTTAATTTTTCCATCATTGTTTTCACCCTTAAATTAATATAAAACATTATTTAAAATTATACATTAATAGCATACGTCTAAATTAAATTATATCTAAAAAAAATAAAAAAATAACAACTCATGAAGTTGTTACTAACTTAATTTAATACCAATAGCAATAGCAATAGTTATAATAATCCCAATTACAATAAACATTGTTGTAATTGTAATGATTAAATTAATTGAAAAATTTTTACCATTTTTTTTAATTTTTGATTCCGTATATTTAACAACGCTAGGAATATTTAAAACTTTTGTTAATTGGTTGTTTTTATCATCAAAAAAGCTTTTTGTAGTTGGTTTTAATTGATTTTCATCAATATTATTGGTTTGTTTAACACTATCATTCATCATAAAAATTATTTGATTGATAACTTAATACTTCTGTAAGTTTTGCAATCACGACAAGCTCTATGAGGTAGTAATGCTTTACCACATTCATTACATGCTACTGTATTAGAAATTGTAAGGTGATCGTGACTACGACGCATTCCTTTTCTTGATTTACTTACTCTTCTTTGTTGTACAGCCATATTATCGCCTCATTTCTAGTTTGTGTATCTTATAAGATATTGAATTTTATAACATTTATAAATTATATATGATTTTATTATTTTTTAATATCTTTTTTTAAAAAAATATTTAATTAATTAAAAGGAAAAGCTACGCATAATCTATAATAATAGTAATATGTTTAATGATATTAAGGTTCAAAATTATGAATACTAAATCCCCAATTCTTATACTAATTGCAGGAGCTTCTGGTTCTGGAAAAACAACCTTTGCTAATGAAATTATTGCAAGAATTCCTAAAAATACAACAAGTGTTGTTATTTGTCAAGATTCTTACTATATTTCTAATTCACAACTAAATAAAAAAGAACGCCGCTTAATTAATTATGATCATCCAAGTAGTTTTGAATGAGAGTTAATGCGAGAGCAATTAAGTGACATTAAAAAACGTAAAAAAATTAAAGTACCAATCTATGATTATAAAACTGAGATTCGTTTAGATAAAACGATTGATATTAGTGATGTTGATGTTATAGTTCTTGAAGGTATTTATGCAATTTATGATGATGTCATTAATCAAATAGCTGATTTGAAAATTTTTATTGAAACTCCAAAAGATGAATGTTTAATTCGACGAATTCTTCGAGATGTTAATGAACGTAATCGATCATTTGAATCTGTAATTACTCAATGACGTAGCACTGTTAGCCCTATGTATGATCAATTTGTTGAACCAAGTAAGAAGAATGCTAATGTTAGTGTGCTATGAAATGAACACAATCGTGTTGCATTACATTTAATTCATAAATGAATAAATAATATACATTAAAATATCATAACTAATTTAATTTTGAATTATGTCATATTATTATAAAAGCAAATTTAGAATTACAGATCATGCATTAGCTCGTTTTCGAGAACGTGCTGAATGAACTGATGTTAACATTAAAAATCTAAGTGGTACATTCATTATTCCAATTATTAATGAGCGAATTTTAGGAATTCGTCCTTTAAATTCTTTAGACAATAATTTCCATATTTACATGGACCCTAAAAATAAAGGTTATTATTTTTTAGTTGATAAATACACAAATACAATTATTTCTTACACTAAACGAACTAATAAAAATGATTTTTACCATATTAAAAAAATTAAATAAACTTTATTTTTTAACATTATCAATAGTATCATAAGTAAATGTGAAATGTTTTTTTTAGTTTTTCTAAACGTAGTATAGTTAAACTTTCTAAAATTCAAGGTAGTTTTGCTTGTTTTAAAATATTTATTAAATTAGGATTATTATTAATTTACTGGAAAAATTTTAAATTTATAATTTTTACTAGTTGTCGCACACTTATTGTTTGTGTGAATCGACGACTAGCTTGTTTACTTTCGCAAACTTTTTTAGTTAAATATTCGCCAATTTCTGGATTTTAACCATAAACAATGAATAATAAATCAGCCATTTTATCTCAAACGCCTACTGCTAAAAAGGTTTTTATATCACTAAATGCTTTAGCTTTTTCTAAACTTGTATCATTAAAATTGCACCTCATGTTTTAGTATTAAATGATACTTGTTTAACTTCTAATCATTGGTTAAATCCTTGCTTTTTAGCATCTAATCGGTAATAAACGCCTACAAAACCACCTTTAAATTCGTTTAAAAAAGTATCGTTATCAAATTATTTATATTTTTCTTGAATTTCCATAATAGGAATCATAGCATTAAATGATTCAGCTAAAAATTCTGAACTTATAGGTTTATAATCCCCTTTTTAAAAATATCTAAATCTAAATCATTAAATTTTTTAGAATCCATAAACTACTATCATTTATCTCTCTTTTAAAACTTATGATTATATTTTATACCTTCAAAAGTGTATTGTTAATGTTTTCATTTGTGCATATTAATTTGCTTAATATTTAGAAAGACATAATCAAATGCACTAATTATTGAAGATAATAAAATGATATAAATTGGTGTTTTAATGCTTTCTTTAATAATGCGTGGTAATAAATAGTAATTAACTCCGTATTTTAAATAATTCGTAATTGGGACTTTTATATATTTTAAATACGCTACACTTACGTAAGGTCCTAGTGCAAACGAAAATAACATTGTTGAAAAAATTACTAATAATGAGCAATAAATAAAATTAATTAACTGTACTTTTGAACGATTTCTATTACGTAAATAATAAATGATAACAAAAGCTTCAACAAGCAAAACAAATGTAGCTAAAAAAGCGATTGCTAAATATTTAAAAATACTAATTCAATCTTCATTAATTTCTTTATCACGTTTTTTAAGAATGCTAAATAAGGAGTTTTTGCCATCATTAACTAAGGTCAAAATCATAAACAATGTTAATATACTAAAAAATAAAATAAAGATTTGATTAACTATTAGATCAATTATTGGTCGTTTAGTTTTTTTTCTTAATGTAGCTACAGATCCTAGAAATCCAGCAATAAAGCCTACAATAGGTTCTTGAATAGCATACATTCAAAACCAAATTCCACCACTAATTAATCATGCTAAAGTATCAACTAAAAATCCAGCAAATATTCCAATTAATGGTCCAAAATACCACCCTAAAACCATTAATGCCATTCATGTAAAAGAAATAACCATATTAAAACCAGGAATTTTAATATGTAAAAATCCCATTCCTAAACGAATTGCAACTAAAACAGCAAAAACCACGATTAAATTAATCGTTTTAAAAAGTTTTAAAGGATAAAAGGGAAATAAAAAAAGCAAAAATTTTTTTTTATTTTTTTGCTTTTTATTAGCAATTAAATCCTTTGTAAAGGATAATGCTAATTTATTATTTATATCATAGTTAAATTTAAATCTTGAATTCATCTAAAAACCAAACAAGTCTCTAAAGGGATTTTTTGTTCTTAAATATGTATTAGGATCAATTTTACCTGTTAAAATATCATCATGAATTTCTTGTAATAATAATTTTATTCTTTGAATATCACGTTTAGGATTTAAAATAAAAGTACTAATAATTAAAATTGTTCCTAAAATAATAACTACAAATACTAAAATTTGGGCGATACTATAGCCATAAAAAATACTTTGTAAATACGAATAATCAAAACTAAAACTATTATTTCTTGCTAAGTTAGCTAATACATCATTAATTTGTGCTTGACGAAACATTTCATCAAATGAACTACTTGAAAATCTTAAATTTCCAGTAAAAATATTTGCTAATGCAACTACTAATAATGCTCCAAACAAAATAATTCAAATAAAATTTTTAAAATGAAACTTCTCATTTTCACTAATTTTTCTTTTAAAAGCATTTATTCCAGCATAAATCATAATGAATATTGATATCACTAACTGGGTTGGAAATGTTGGCGAAACTAAACGAACAATATGAGTAATACCATTAACATTAATAACACTTGACATATGTGTGCCAAAATCCATTAGAAAAGCATACGTTAATGTTGTAAAAAAAACCAAAGCTAAACTAACAGCAAAAAAAACATATTTTAAGAAAGGAATAACTTTTGGTTTCGTTTCATATAAATAAATTTCACCATTCATAACCCGAGCACTAAATACCATATTTGTTAAATTATCGATATTTGCTGTTGTAAAATCAGAAGTTGGTTGATAAGTATGATTCATACTATCTTCATCACTAATAGATTCAGCACTTACCTTTTCATATTCTGTACTAGTATTATTTTCTTGATTTCTTGGAGTATAAAAACCCATTGATGCTTGCGTCTTTTCATCATACTTAAAATCAATATCTGCAATTCTTTGTGATTCTTGATCTAAATCTATTTGAAATGTATTTAAAACATAAATTTTTAAACCATTTACAATATCTTTTAATTCATCAAAATCGTTATAATCATTAACTATTTTTTCATAATGAACATATGACTTAAAAGCCTGTCAATAACTATGAAAATTTTTATTTGCTATTTCATAATTATCTTCATAATATTCAAAAATATTCATTACTTTATTTAATTGATTTCGATAATCATTATCATTTTTAATAGCATAATTTAGTTTTTGATTCAAAAAAATTGTGTTTAAATATAATTTAATTGATTTATATAAATCTTTTTGTGCACTTGACAATTCTGCATTTAGCATCTAATAACCACCTCATTAATCTAAAATAAAATAAACTGAACTGACATTAAAACAAAAGTATTTTATTTAATTTGGTCTATTAATTTTAACAAATATAAATATAGTTTTTTTATCTTTATCTTCAATAAACTTAAATTATAAGAACTTATTGTAGTGTTAAAAATTCAAAACTCAATTAATTAATCCAAAATATTTATCAATTTTATTTTCCTTGACTTTAATTTCTAATTGTAATAAATCATACATTTTTTTAAGTAAAAATTTAGCTGAATAATTTCTAATTAATTTTAAATTTAAATCAATAATATAAGGTTTTTGATTTAAAGAGCTAGATATAACGTTTTTGTTATATTTTTTTTCATGTAATAATTTAATTAAGTAATGTGTACTCATTATTGTCGCTAACATATTAATTATTGTGAGTTCATCAATTTTGTCAATGAGCATTTTATTAATTAAATTTATTGATTCATTTATTTTATGTTGATACAAAAGATTTAATAAACTAAAAATTTGATATTCACCATAATCACATATAAAATTATTAATATCAAACATACCATGATTTTTTAAAAAATCATTAATTAATTCTATTTTATCTAATTCATTATTAAGAAAATTAATATTAAAATTTGTTTTTTGAATAATCGTTTGCATTATAGATTTATTTAATGATAAATTACGCTGATTTAACTCTTCTTTTAAGAAAGCCATAGCATTTTCATTTGTATATGTCATAAAAGTAATTTGATGATTAAATTTTGCAATTGTTTCTTGAATTTTTTTTGCTGTAGAAATTTTTGTAGATTCAATTGTAATAATAATAGCAACATGATTATTAAAAAAATAATCAATTAAATCACTAACATTTTTTAAATGTTCTAAATTTTCGAATCAGTTAACGTTTTTAAATAAGTAGATCTTATTACTATTGAATAAATCACCTTGTTCAATAAGATTTTTAAAAGAAATTAATGAAATTTCTTGATCATTAATTTTAACTAATTCATCAAAAGAGGTCGCAATTTCATTAATTTTTTGATCCATTTTTAAACGATTGTCACCAGTAATTAAAAAAAGTTCTTTATTTATTAAGTTCATATAAAAAAATTGCTGCTGCTACTGCAACATTTAATGATTCTGTTTGATTCATTTTAATCATTGTTTTAATGTGAGCATGATCAATAAAAGTTTGTACCAAACCATGACCTTCATTACCTACCATTAAACAATATTTTTGTTTAATCGTTATTTCGTTTAAAGAAATTGCGTTTTTATCTAAAATAGTAGCAATTAAATAGAATTTATTTTTTAATAAAAAATTTAATGCTAGTTTAGAATCATTAAAACGTAAAAAATTAATTTTAAAAATGGCCCCCATACTTGCTTTAATTGTTTTAGGATTATATAAATCAACACAATTATTAAAAATAACTTGTTTAATATTGAAAGCAAAGCAGGTTCTTAAAATGGTTCCTAAATTACCTGGATCTTGAATATTATCTAACAATAAAATATTATCGTTAATATCACACATTTCTTGTTTTATTTTAACAACTGCTAAAATTGGACTTGGTTCGATATTAACTGATAGTTTTTTAATAATATTTCCACTAACTTTATAAATTTGAATATTGTCATATAATGTATATTTAAAAATCGTTTCAAATTCTTTATTATAAACATGTTCATTAATAATAATTGAAACTATTGTTTGTTGATTTTGCAAAGCTTCATCTAATAATTTAAAACCCTCAATTATGAATAATTTATTAGCTTCGCGATATTTTTTATCATGAATTAAACGATAAAAATGAATAATTTCTTCATTTTTATTAGATGTAATAATTTTTAACATAAATTAAGATTTTTTAGTAGGTTTAGTCGCAGTTTTTATTTCAGTATCGTTTTTTATTTCTTTTTCTTCATCCTTTTGTGATTTACTAAACTTTTGGAATTCATTCATTACTGAATTAAAATCGAAACTAGATGGATCGTTCGCTACGTTTCCTATTAGATCTTTTAAATCATTCATTTGCTTTGCAATACTTTCAAATGATTGGTTTGAAAAATTTAAATAATCACGCGCTAAATCCTTAATTAAATCTTTTAAAAATACATTTAATTCTAAATTATTATTAGGATCCATTTTATCTTTAATTTTTTTTATTGATGCTAATTCTTCTTCATTAAATTCCAATTGAATTATTGTCTTTTCCATATTTTCTCCTTGATTATGTGCAAAATTCGCTAAATTGTCGTAAATATTTTTAACATACTAAGCATGTTAGAAAAATTTTTACTATTTTTATATTATATAATTTTAATTGATTATTAAAATCATTTAATTATAAGGAGCAAAGTTTATGGATTTATGAAAAGAACAAGATGAAAATGGAATTAGCCCTAATGTATCTAACGAACGAAAAGTAGTAGTTGCTTCAAATGGCGAAAAAACTTTATATATTTTCATCATAGTTATTTCAATTATTTCAATTATTGGTTGAATCTTTTTATTAGTTTGATGATTTAATACTAAAAATCATTTAATTCAAACTAAAAACAGTGTTAATGAAGCTTCATCAAGCATCCAAGTAGCACAAACAAAACGGTTCGATTTATTAAATAAAATGATTGAACAAACTAAATCATATTACAAATATGAAGAAAAAGTATTAGATGAAATAACTAAAAATCGAAGTATTCAAATGTCAAATGATATTAACAAAAATGAAGAAGTATTAAATAAACTTCAAAATTTAGTTAATGTTCAATTCGAACGTTATCCAGATTTGAAATCATCCAATATTTTAATGGAATTGATGAGTACATCAAGTTATTTAGAAAATGAAATTGCTTCATCACGACGTGCTTATAACTCACGTGCAACTGATTGAAATATTATGATTTTTCAATTTATGACTGTTATCGTTGCTGAAAAATTAAAACTTGAAACTTTCCCAATTTATGCTGCAAACAAACAAGAGCGTAAAGATGTAGATATGAAATCACTGTCAGATTTTTAATATAAAATCAAAAAAACATGAAACAACTCTTTAAATAGAGTTGTTTTTTATTTTTATTACTTTAAAATAATTTAAAATATATTGATATTTTTAAAAAAAGTAAAGAGTGATTGTTATATGGATAAAAAATTCATCCGTAATTTTAGTATTATTGCCCATATTGATCATGGGAAGTCAACATTATCTGATCGAATTATTGAATTTACAAATACTTTGAGTAAACGTGAAATGACAAACCAAATTTTAGATTCTATGGATATAGAACGTGAACGTGGAATTACTATTAAATTAAATGCTGTACAAATTAAATATCATGCTAAAGATAAACATGAATATTTAATTCACTTAATTGACACTCCCGGTCACGTCGATTTTACTTACGAAGTTTCACGAAGTTTAGCAGCATGTGAGGGAGCAATTCTAGTTGTTGATGCTGCACAAGGAATTGAAGCCCAAACTTTATCTAATGTTTACTTAGCATTAGAAAACAATTTAGAGATTGTTCCAACAATTAATAAAATTGATTTACCATCAGCAGACCCTAATCGTGTTAAAAAAGAAATCGAAGACGTTATTGGTTTAGATACAGCGGATGTGCCTTTAATTTCAGCAAAAACGGGGTTAAACATTCAAGATGTATTAGAAGCAATAATTAAACACGTTCCACCTCCATTAGATGCTAAAGATGATGCTAAATTACAAGCCTTAATATTTGATTCTTTTTATGATTCTTATAAAGGTGTTGTTTGTTTAGTACGTGTAAAACAAGGAACAATTAAAGTTGGAGATAAAATTCGTATGATGGCTAACAATAAAGATTATATTGTTAGTGAATTAGGAATTAGAACACCAAAAATTGTTAATAAAACAGAATTGGTAGCCGGAGAAGTTGGTTGGGTAGCTGCAGCTATCAAAACAGTCAAAGATATTAATGTGGGTGATACTATTACTCATACCAATAATCCTGCTGATAAACCACTACCAGGTTATAAAAAAATTTTACCAATGGTTTATTGTGGCTTATACCCAATTGATACTAGTCAATATGATGATTTAAAAGAAGCTATGGCTAAAATCTCCTTATCTGATGCTGCATTAACTTATGAATATGAAACATCGCAAGCACTAGGATTTGGAATTCGTTGTGGATTTTTAGGTTTATTACATATGGATGTTATTCGTGAACGAATTGCTCGTGAATTTAATATTGAATTAATTTTAACTGCCCCTTCAGTAATATATAAAATTGAATTAACAAATAATCAAGAAATTAGTATTGATAGTCCTGCTAAAATGCCTGAACCAACTAGTATTAAACTTATTAAAGAACCATTTGTTAAATTAGCAATCATTACACCAGATAATTATGTTGGTGCAATTATGGAATTATGTCAGTCCCGAAGAGGAATATATGATGATCTAGAAGTTATTGACGGTACAAGACGAAAATTAATTTATAAAATGCCATTAGCTGAAATTATGTACAGTTTTTTTGATTCACTAAAATCAATTACTAAAGGATATGCAACAATGGACTATGAACTAATTGGTTACCAAGCTGAAAAATTAGTCAAAATCGATATTATGTTAAATGGTAATAAAGTTGATGCTTTAAGTATTATTGCTCATCGTGATTTTGCTTATGGGAAGTCAAAAATTATTTGTGAACGTCTAAAAGAGGTTATTCCAAAACATCAATTTGAAATTCCTATTCAAGCTTCAATTGGTTCAAAAATTATTGCTCGTGAAACTATTAAAGCAGTACGCAAGGATGTGATTGCTAAATGCTATGGTGGAGATGTTTCAAGAAAGAAAAAACTTCTAGAACAACAAAAAGAAGGTAAAAAACGTCTAAAAGCGATTGGTAATGTTGATGTTCCTCAAGATGCTTTTGTAAAAGTTTTAAGTGAAAACTAATATATATTATTTATGTTAAGTTTTGTTAAAGAATTAAATTTTGAAATTAATCAAAAAAAATATTTTATTAAAATTTATTTACAACCAAGAATTAAATATATTAAAATCAAAATCATTAATCATCAAATTGTTTGTTTGATCAATCACTTAAAATTAATTAATATTGCTGAAGAATTTATTAAAAATAATAAGTTAAAAATTTTAAAAATATATGAAAATGACTTAAAGAAAATTAAGTATGATGAACAATCTTTAGATTGAATCATCTTATTAGGAATAAAATTTACGACTATAAAAATAAAAAATAACAATTTTCATTGTCAATTCAATTTTCAAAAACAAATCATTTATCTTTATGATCAAAATCAGAATTTAACTAATACAAAATTGCTTTATCAAAAAATTTTAATATACCTAGCAGAAATAATTTTTCCACAAATTATTAAAAATGCCCAAAATATAACTACTCTAACTGTAAAAAAATATGTATTTGGCTTTTACAAAAGCCAATGAGGTGTCTATAATAAAATAAAACACACGATTGGCCTTTCGTATTTTTTAGTTCATTATGATCAAGATATCATTAATTATGTTGTTATTCATGAATTAGCCCACATAAAATATCAACATCATCAAAAATCCTTTTGGGACTTTGTTTTAAAATATTGTAAAAATGCAAAAATATACAACAATCAACTTAAATTTTAATATTTAAAAATGATTATAATATTAAGATAAATACAATAAAAAATTAATTAAAAAAGATAGGAGTATGACCCTATCTTTTTTAATAATATATGTGTGTCGCAATACTTAAACAAATGGTAATGATTAATAAAATAACACTAATGAAATAAAATAATCAAATAAACACATGATAGTATAATGATTCGTGCACGCCATTAATTCCAGTAATTCAACAACCTAATAAAAAAATAATTGGTAAATAAATTAAAGCAAAACCGATATAATCTAATATTTTAGTAATATTAACTAAGTATTTATTGAGATTAATAATAAAGCTTAATTGAATACTTAAATTATTTGAGTTAAAAAAATGTAGAATTAAACCAAAACCAATTGATAATAAAACTAAAACACCAAAAATAATTGATCAAGAAATAATCAAACCTGTTTTTTGTCGTAAATGTTTTTCTAATCAGTGTTGTTCTTTATTTTTATTTTGTTTTTTTAACATTGGATAATTAATTTTCTTGTTGTTTAATTTTTTTAAATAATTCATCAATATGATTAGCATAATCAATAGTTTCATCGATTACAAAGCGCAATTCCGGTACTTTATAACTAGTTCATTCCGCAGCTAATTTTGAACGCAATAAGCCACTAACTTTATTAACATTTTCTAAAACTTTAAGCATACTTTCGCGTTTATGAGCGTCTAAAAAAATCTTTGCTACTGATAAATCATTACTTAAACGCACTGCAGTTACACGTGCTAATTTTGCAATTTTATCATTAATTTCATTCGCTAACGCATTATTAATAACATCTTTAATTAAAGATTCTAACCTTGCAACTCGAACTTCGTTTGCCATATTTTATTTATTTAACCTCATCATGTTTCTTTTCAACTTTAATATAAACTTCAATAATATCGTTCTCTTTAATATCATTAAAGTTTGCAATTGTTAATCCGCATTCTTTATCAGCAATAACTTCATTAACACTATCTTTATTGTGTTGTAATGTCGCAATTTTTGAATTATATATAACAATACCATCACGTAAAACACGAACCAAAGCATTTCTTTTAATTTTACCATTAATTACTCGACAACCACAAATTGTTCCAACTTGACTGTGTTTAAATAATTTAAGAACTTTGGCTTCGCCAATCACTTCTTCAATAATAATTGGGTCTAATGTACCTTTCATTCATAATTCTAAATCTTCTTTAAATTTATAAATAATGTCATAATTCATAATTTGTACACCGACTGAATCAGCTAAATCTTTAATAATTCGACTAGCACGGACATTAAAACCAATAATTATTGCATCAGAAGTCTGTGCTAAACGCACATCACTTTCACTAATTGTTCCAATTGCACTCCGTATTAGTGTCGTAGTCACACCTTCAATGTTAATGCTACTAAAAATTCCTTTTAAAGCTTCAAGTGAACCTTGAACATCAGCTTTAATAATTAAATTAATATTTTTTAATTCACCATTAGCAATTTTTGTTCGAATATCCGAACTTTGTAACATTGCACGTTCTAAACGCATTTTCTTTTGTTTAACATCATTTGCTATTGCACGTGCTTGTTTTTCATCAGCTAAAGCTAAAAATTTATCACCGGCTGTTGGTACTTCTTCAAATCCAGAAACTTTAACAGGTTTTGAAGGTAATGCTATTTCAATTTCGTTGTCATATTCATCAAACATATTACGAATTTTTCCATATGTTGAACCAACAACTAAATAATCACCTTTTCGTAATGTGCCATTTTGAACTAACAATGTAGCAAGAGGACCATGACCTTTATCCAAATTTGCTTCAATTGTAGTTCCATAAGCTAAACGATTAGGATTAGCTTTGTAATTATTTAATTCAGCAATCAATAAAATTGAATCTAATAATTCAGAAATTCCTTCATTTTTCAATGCTGAGCCTTTAACAAAAACAACATCACCACCAAGCTCTTCAGCAACAATTTCTTTAGCACTTAATTGATTTAAAACTTTTTCAGGATTTGCTTCGTATTTATCCATTTTATTAACAAAAACAATAATAGGAACATTAGCAGCTTTAGCATGATCAATTGCTTCTTCAGTTTGCATTTTAATACCATCATCAGCAGCAACAACTAAAATCACAATATCTGTTAAATTTGCTCCGCGAGCACGCATTTCAGTAAAGGCTTCATGTCCGGGTGTATCAATAAAAGTAATTGCTCTATTGTCTTTTATAATTTGATAAGCTCCAATATGTTGAGTAATTCCTCCAGCTTCTGATGCTGTTATTGATGATTTACGAATAGTATCTAAAAGTGTTGTTTTACCATGATCAACATGACCCATAATTGTTACAATTGGAGCACGAGCAACCAAATCAATTTCTAGATCATCAAAATAAATATTTTCTAAAAGATTTTCAGTGTTAACATTCTTTTCAATTTTAAAATCTAAATCATATTCTAAACATAACTCACCAATTTGTTCTTCATCTAAAATAGTGTTTAAGTTATAAACAACACCTTTTTTGAAATAACGCATAATAATTTCGTTTGGACTTTTATTTAGTTTTGGTGCTAATTCTGAAATACTAAGAGGTGATGTGAAAACAAATGTACCATCAAAAACCCCAACATCAACTTTTTTAATGTGTTTTTTTACATCGATTGCTATCCGATTATCTTTCTTTTGTTTGATATTTTTTTTCGCCATATTTTTACCTCTTACTTATATTACTCTATATATTTTAAATTAATGATTGAATTAATTGCTCTAAAATCCGATAAACTTGTTCTTCGTTTTTAATCATAAAACGTTTTTTTAGAAATTGACACACCTTTTGAATGTTTGTATTCTTTTGAATCATTAAATAATACCCACGATGATAATTCAAACTAAAAATTAACTCACCGTTAATAATAACAAAGCGAACTAAATGATCTTTAGGATATTTTTCTTTACTAAAAACACAAGTTCTTTCATTAACTTTCATTGCCATAATATTTAAATTAGAATTTAAAATCTTCGAATCCTTCAAGATATTCTAATTGTGTTTCATCATTTTCACCATCTTCATCATTTGAAGAAACAACTTTTTTTTCTTGTTGTTTTAATGGGTGATTTAATTGGACATCTTGTTCAATTTTATCATCTTCTATACTATCAATTAAAGCTAACATTTCATCATTTGTGACATTTTTTTTATTAATTGATTCAATATTTCATGAACGATAAATTTTTTCATCAAATTTTTCGTATAAAATATTTTCTTCGAATGCTTGCTCAATTGTTTTTACATCAATATTTCATTCAGTAATTTTAGCAATTAAACGAATATTTGCTCCACCACGACCGATAACACTTGGTAAATCATCCTTGCTTACAATAGCAATTGCAGAACGTTGACCACTTTCATTTTCTTGAACTAAAAGTCCTTTTAATTTATCTAAACCAATAGCATTAGCAATAAAAATACGTTTGTCATCAGCATAACGAATAATATCAATACGTTCATTATTTAATTGTTTAGAAATTGACGTTATAGTTAAACCTTTATTTCCGACAACAACAGCAACTGGTTCAATATTTAATAAATTAGTGCTTACAGCTACCTTAGTTTTAAAACCAGCTATTCGAGCAATTTTTTTGATTTCAATATTTCCATTTTTAATATCAACAACTTCGCGTTTTAAAATTTCTTCAACTAATTCTGGCTCTGTTCTAGATAGTAAAATTGGCCATAAACGGCTTTGTTCTTTAACTTCTTTAATAAGAAAATCATAAGTTTCACCAACCTTAAACTTTTCATCACCAATACGATCTTTACGACTTAAATAACCTAATGTTTTTCATTGGTCATCTAGTTCAACTTTTACTCCAGTAATTTCATTACGTTGTTTGTTATAATTAACTTCTGCAACTTTTGCACGAATCATTTTCATTAAAGATGGTTTTCAAAAATCAGCTACTCTTTTATTATGAATTTCAGTAATTTTTTGACGCAATAAACTTCCAATTTGACCTACTTCAATACTTTTAAAATTATCAATTGAAATATGTTGTTTAATAAGATCGCCAATTTGAGCATTTGCATTGATTTTACGAGCATCATCTAATAAAATTTCAACAGCATCATCCAAACATGTTTCTAAATCTTCATTATGTACTTTTTCATTCTCAACGACCACTAAATTTCGAAAACAATTAATTGTTCCTTGTTTAAGATCAATATTTGTTTCAAAATTTTCACCAGGATGTGTTCGTGAATAAGCTTTTTCAAATGCTTCTTTAATAATTGAAGATAAAACTTCAAATTCAATTTCATTTTGTTTTGCTGTATCTTTAAAGTATTCAATAAATTCTTTTGATTTAAATGAGTTAGACATTTTTTATAACCTTTCATATAAATAAGAAATGACAACCCTAAGGTCGTCATTTCATTCACATTTTACTTATTGGTTAGATTATATCATAACATTCTAAATATTTTTTAAATTTCTAATTACGTTTACTTGCATCAGCAATATTTGATCTTCTTAAGATTCAAATTGCAATCGCTCACATTAATAAGAAAATGATAGCAACAATTAATAAAATAATTAAAAAATGTCACCAAATAAAGCTAGTTGTTAATAAAACATTTAAACTATTAAATATTATTTCGCTAAATACTTGCATTACTACAATAACTAGTGGAATTGAAATAATTGATCCAATTATTAATGATGGTAAAAAAGCCATTAAAAATAACTTAGCATTCTTTAAATCACTATAACCAATATTTTTTAGTAATTTAGCGACTTTTAATGTATCAGACAATGAATTAATAGAAATTAAAACAAGTACTAATATAATAATAAATAATATTAAAAAGATCACAACAATTTCAACAGTTTGAATTGTTTTTGAAGCAGTTTCTAAAACTGCAAATAATACGTCAACTACTTCAACATTATTTAAAGTAGATGAATAAGTATCACGCCCATAAATACTGATTAGTTTATCAATTACCCATTCTGCTTGAGCTTGTTCATCTCTTAATCCTAAATAATTATGGTTGTCATCATAAATATTGCGTGCATCTAATTTAAGTTCATCAAAATTTTTATAACCTAAGGCTTTAGTTAAAGATAAGCGAGAGTTTAAAACTAATGATTTGGGATCTCATTTATTATTTAATTCATATGTAGTATATTTATCACTTAAAATATTTTTAATTAGATTAATAGTTGGTGCATCTTTTTTTCATGTTTCAGAAATAGGATATAGTCCCGAAGGTGAGTAAATAGAAATAATACGTGTTAACATTGCTGGATTATTTTTATTTGTAAAAATTCCATTGAAAGCACCATCGTATTCATCATAAAGTGATTTATCAGCATATGGCGAACTATGCATTCCTAAAATTTCAGCGGCATTTTTAATCCCTGTATAAATTTCGATCCCCTGATAAGTATCAATTATACCAACTACTTTTAAAGTGATTTGTTTTTTTATGTAATCTTTGGTATAACGATTTGTTTGATTATTAGCAACAAAACTAAAATGATCACCAATTTTTAAATTGTAATATTTGGCAGCTACTCGATTAATAATTGCTACTTGCTCATCATTTTTATTAAATAATAAATTATTAATCTGTTTTTTATTGGATAATAAATGCACTAACTTTGAATTCTTAATAATTCCAATAATTTTAGGATTTTTAGCTTCTTCATATTTTTGATTATTAATTTTATTAATCTGAGCATCAATTCAAATATAAGGTTCATCAAATTCGTTTAACTGTGTTTTATCATTTTTTGTTAAATAATCAAAACCTACCATATTAAATGTGATACCTGACCAAACATCTGCATACGATGGATTAATTGATGGATGAGTTAATAATACTAAATTAATAAAATCATTATCCAATTTAATAGATAATCCTGTTTTAATAGCATTTGTAGCTTTTGCTGGATCAATTCTATAAGAACGTTTAATGGGTTTTGGAGAATAAAGATAAATCTGTTGATTAATAATATTTTTAACTTGATAAGTTAAATCATTGATTTTAGTAACAACTATATCATTATCACTTTTAAAATTAATTTTTTTAATGATTTTATTTTCGTCATTTTTAAAAAGAATTTCATTATTAATTGCATTCTCTAATAATTCTTTTCTAACAATTGTTGCATTAAAATATTTTAATGGTAAATAATAATCAAAATCCCCTTCTGGATCAAATTTTAAAAAACGTGCTCTAAAATAAGAACTAAAAGTTTGATAAGTTAGTTTATGATAATTTTGTTTGCTATTACGTTCAAAATAAACTTCATCATAAATCAAATCATCATTTTTAAAAATATATTTTTTTAAATCATAATTTTGCTCATTAGGTTTTTTATAATAAATAGCACTAACAAAGTTTTGATCATCAATTGGAAAATTATTATCTTTAAAATTAATTATTCATTGATTATTTTTAAAAACCAATTTTCCATAAATATTTTTATAATTAAATAAATTATTATGCACGCGTACTAAAATTTCATACGTAACATGTTGATCTAAAAAAGACTTATTAAATCGTTTTAAATATGTATCAACAATACTCTTAAATGTAAAATAATTAAAATATTCATTAGGATCATAACTATTACGAATATTTAAAATATCATAAGCTAATTCATTAACACCTTTTTCAATTAATTGTTTCGAAAAAATGCTATGGTCATTAATTAATTTTGAACATCATTTTTTATAAGTTTCATTTAAAATTTGTAAATTATTTTCAGGTATTAAAGACTTTGTAATACTTCATGGATTACTTGAACTACCACTATTAACATCACCAATGCCAAAATTAAAATCTAAAAATTGCTTAACTTGAAAGGCATTAGCAATATAGTTTAAATCATAAATTCGACGCTGGTCATCTTTTGCACCAATTAACTTTAAATTCTGTAAATTTTTAAATAACGGTGAATTAATAAAATCTTTATAATCACTACTTTCATATTTCTGTGTCAATTCATGATTATCATTATCATTTAATGTAGAACCAATTTGATTTGTACTTAAAGAAATATATTGACCACCTTGATCTGTTGGTGTTATTAAATCAATTCCATAACGATATTTTTTTGTTTGTAATGTTGCATCTATAGTTTGTTTAATTTTACCTCTTGTTCCAGTTAAAAACATAATCGATCCAAATGATAAAGCAATCATAATTGAAAAAATAATAATTCTTGTAATTGATGAAAAAGATAGTGTAATTAAATACTTATTAAAACCACTAATATATTTAAATGGAAATTTAATTACATAAACAATTTTAGAAATTTTAAATGTTTCAGTTCCACGCATTAAATCAATTGGTGGTTTTCTTATTGATCAATAACCTAATCCAACTATTAAAATAAAAAACAACAAAAAAGGGAAAATAATAACTGTTATTAAAGCACCAAAATTAAAGTTTTCTAAAACTGTAGGAATTAATCAATAACCACTAAAAATATTAATTGCTAAATTTTGAGTAAAATAAGCTGTTAAATAACCGCATAATCCACCAATAACACATGGAATTAATCCAATAAAAGCAAAACTTAAAATGATTTTTCAACGAGGAACACCATTAGCAATAGAAATACCAATATTAATTTTATTTTGTGCTAAAAAACGGTTGATGATGATAACAATTACAAAAATTGTTAGAATCAAAATAAATAAAGTTATTAAAACACTAAAAGTTTGTATTGTGTTCGTAATTTTGTTAATGAAAACTAATCTTAATGGGCTTGGTGTAATATGATTATTAGTATCATCTTTTAAATAAGCAGCATCTAAACTTGTTGGTCAGTTCATGATAGCATCTTTTTTAATTTGATCATTTAAATTTTTAACAATTTTTTTTAAAATACTGATATCAACGGATTGATGCTTTTGATTTGTGATACGAATTTGTAAATTATTTTCAATTGGATTAGATCTAAAAGCATCGTAAATACGGGCATATCCATTTAAGTTAACATATACTAGTCCTTCTTTTTTTGGATTAGGAATAACAGAAGTGAATGAGATAATCGGATACATAAAATCAGGAGTAATACCACTATCTAAAATTAAATAAGGTGTGTTATCAATATGAATAATATATTTTTCATCCAAACTATTGAATCACTTTTCAAAACTTTGTTGTTCTTCGTGGTCGACTAAAGCTTTTTGTCATAAATACATTGGGTATTTTTGTTTGTTATGATTAGACAAAAAAGCACCTAAACCAACTAATGCGTATGCAGATTTAACATCAAAATAGACTGGAATAGGAATCCCTGCGGGTTCAAAACTAAAAGTTATTCGATGCCCTTTAACAATAGTATTAACATTACCATTTATACTTATACCTTTTTTTGATGGATATAACATTAAAATGAATTGTGTGCTAATCTCTTTTGCTTCTAAAATATCAAAATTAGCTTTGTTGTTTAATTTTATATTACCAATTAAAGGATCGTAATTTTTATCTTCTAGACTATATTCATAAGCTTTTTTAAATTTTTTATATAAATTATTGGTTTTTGATGGTCATGCACCATAAACAATATTTTTTAATAAATAAAGTCGTGCTTGTTGACTAATACTAACATCGTAATTATCAACTTTAGCTAATTTTACCAAATTACTTTTTTGTTTATTGTCTAATTCTTTTAAATTATTAATGACAAAACTGGCATTTAAAGTTTTAGTTTTGATTAAATCAATAACATCTACTTTAGCATTATTTAAAATCCCATATAAATCAATATCAGAAAAACTATTTTTAATCAAACGTTCACTTGTTTGTTTATCGAATGTATTAATCGTTTTACTTGTTGAATACTGAACAATTTTATAAGTAATATCATCTTTTGTCGAATTAACAATCAATGAATTAGAACGAGTTCAATCAAAATTCAAACCTTTTTGTGCATAAAAAACTTTTAATTCTTCTAAAAGCTTATTGAATTGAGCTTGTTTAATATCTTTTTGATCTTCAATATAACTTTCATTAACAGTTATATCATGTAATTTTTGTCTATTGACTAAGTTTGTATATGATCTTGTTAATGTTTTAGAAAGATTTGTTAACATACTAAAACTAAAGATTGACACAAACAATAAAAGGCAAAGACCTATAAAAGAGGCTTTGCTTTTTAGCAAATAACGATAGACATTCTTAATTAAATTTCTAAACATAATAATAATTTTAAGCAAAAAAATGTTTTTATCTTCTCTTTTTTTGTTTTCATTGTTTAAAATTATACTTTTCAATAAATTATTTTTTTATTATGTTTATAAAATTTATTAGAACATAAAAATAATTTTTAATGCTTTTTGATAATTAAAATTCTCTAAATTAATGAAATAAATTGATTTTAAAATTTAATTCATATGTTAAAAATTTTTAGTTTTTTAATTCATTTTCTTTTTCTAATAGATTATTTAAAAAGATCCGTAAATTTCTATCATGAGATAAAGTGGGGTAAGAACGACGTTTGTTTTTAATTTCAATAGGATGAATAATTTTATAATTTAATCAATAAACATGACGAATTGGGTCAAATTTTGAAACTAAAAATAAGTGTTTACTGTTAATTAAAAAACGCGAAGAAATTGTATTTCAATGATAATCTGTCAAATTAATAAACTTACAAACTCCTTTTTTATGACTATTAACAAAATCAATTTGAAGTTCATTATTTTTAATTTCACGAACAATTCCTTCAATAACTCTTAAAGGGTTTTTAAAATTATTAATATTTCTATTTTTTGAAAAATTTTTCATCTTCTATATCCTTACTATTTTTATTTCTATGTTAATGATACTAATTATTTCTAAGTATATTTTATTGGTAATCAATTATTTTTATCAAATATAAAATAAGACAAACATAATATAATTATAAAATATTAGTTAAGGATATTTAGATTAATAAAAAGGAGAGGTAGTATGAAATTAAATGTAACTATTGAAATTCCAAAGAACTCAAATATTAAATATGAATATGATCGTGCAACTAAAGAAATCATGGTTGATCGTATCTTACACGGTTCAATGGTTTATCCTCATAATTATGGTTTTTTAAAAGAAGCATTAGATTATGATGGTGACGAATTGGATGTTTTGGTTTTTGCTGATCAAGCATTCCAACCAGGAATTAAAGTACCTGCACGTGTTTTGGGCGCCATGAAAATGATTGATGGAGGAGAAACAGATACTAAATTATTGGCAGTTATTGATGTTGATCCTCGTTATAAACACATTAATAATTTCAAAGATATTCCATTACATTGATTAGCAGAAGTGCAAGATTTCTTTGAAAATTACAAAAACTTACAAAATAAAAAAGTTAAAATCTTGGGGTTTGAAGATGAAATATGAGCTCAAAAAGAATATGAAGAATGTGTTCTTTTAATGAAAGAATATGGCCATCTTAAAAAAGATGAATTTGTAACTAAAATGATGAAAGAACGTCCAGAAAAGTACATTAAATAATGCTTCAAAAAATCAAAAATTCAAATTTTTATATAATAATTAAAAAATTTAATCAATCACTTAGAAAAATAAATTTTTTTGAGAAAATTATTTTAAAAATAAACAATAAAAGCATTCGTGATTACTTTTTATCAATAACATCATTAGATGTAATTTTTTATAAAATAATTAATTTTATTATTGTTGCTTTAATTGTGCTATTATCAGGTTTTTTGGTTCGTGAATGAGCTATTGATTTAGTTGTCAAAACAAAAGAAACGCATCATGGTCCTATTTTTCATTTTACACTTAATAATGGGATTGCTCTTGGTAGAATTAATAACCATAGCGGAATTGTTTATACTTTACAATCAATCCCCATCATATTAGGATTTTTATCATTTATTTTTTTAAAAAATTCTTGTTATTATGTTCCATTGCTTTTTCTATTATTTGGCGGTTTAGGAAATATTATTGATCGCTCAATTCCAGAATTTGAATTATTCAATGCTTTACCAAATATATTTCACAATAGCCTTATAAATCCTAATAGTGTTGGTGGAGGCGATGATGTTATTAATGGTGTTGTTGATTATTGAAAATTTGTTAATAGTATAATTAATCTTTTTGATGTTTATATTGTTGTTGGTGTTTGTGTATTAGTTGTTATTTTAGTTATTAGATTTATTATCAAATGAAAAAATGATAGTGATAATCAAGACATTATAAAAAAAGTTGCCGATGAGAATGATTTAGAAATCAACGAAATTTCTAACAAACCATTCGGATATGATGACTTAAAAAAATCATAGCAATGAAAATACTATATTCTTTAATGTCGTCTCAACGCATCGATAAATTTTTAGTAGAAAATACTAATTATTCACGAACATTAATTAAAAAAATGTTAGACCAAAACCTAATATTAGTTAATGACAATTTTATTAGTAAAGCAAGTTATTTATTAAAAAAGAATGATTATATTCAAATCAATAACGAACTACCTATAGTGCAAGAGCAAAAAAAATTGTTACCGTTTGTATATTCATTAGAAATTATCTTTGAAGATCAGGATCTATTGATCGTTAACAAACCTTCTGGTATGTTAGTTCATCCAACTAGTTTTCATGAGCAAAATACATTAGCAAACGCTTTAAAATATTATTTAAAAACTGATAATTTTTATATTACACATCGTTTGGATAAAAATACATCTGGATTAATTATAATTTGTAAAAATCAAATAGCGCTTAATTCTTTGCAACAACAATTTATGCAAAAAACCATAAGAAAAAGTTATTATGCTATTGTTCATAATCGTTTTGATATGCAGCATTTGCATTTTATGATCAATGAGCCTATTGGCCATAGTTATCAAGATAAATTAAGAATGAAAACAGGCAATTCTAAAAATACTAAAGAGGCTATCAGTATCGTTAAAGTACTTGAGCAATATAAAAATACCGCCCTTGTTGAAGTAAGTATTATTACTGGTAGAACTCATCAAATACGGGTTCATATGCGTTATATTAATCACCCTGTTTATAATGATCCTTTATATGGGATTAGTAAACATACCACAGAATACGAGCAATACTTGCATAGTTTTTATATTAGTTTTAATCATCCATCCACTAATAAAAAAGTAGAATTTAAAATCAATATGCCAAAAGAATTTATGGAATTAATTAGAGAGTTAAAAGCAAATGAATAGTACATACCAATTGTTTATTGATGTTACATCAAGAAAATGTATTTTAGCAATTTATAAAAACTTTAAAATCCATACTAGCATTATTGTTGATACAAACAATAATCTTACAGATATTATTGTTGAACACCTTAATAATTTATTAAAAATCACTAACTTGCAATATCAAGATCTAGAAGCAATATATTTAAATACTGGTCCAGGATCATTTACTGGAATACGTGTTGGTGCTATTGTTGCTAAAACGATTTGTACAATTTTTAATAAAATTAAACTTTTTATTAATGATAGTTTAAATATTATAGCCAATGGTAAGAATGATGTTTTTGTTTATTTAGATGCTAAAGGTAATAAATCGTATGTAATAACTATTACTAATAATATTCAAAACGAATATAAAATTATTACTAATAAAGAATTACAAAATGAATTAAAAAATACATCATTAACAATAATTGATGCAAATCAAATTGACTATCATGATCTCATATATAACTTAAAATTTGATAATTTTAAGTTAACTAATATCTTAGATTTTAGATTAAATTATGTTAAAAAACCTCTTAATTAGACCTGCTGATCAAAAAGATTTGAATGATATTGCTATTTTTGAAAATAGATTTTTCTTAAATGATTGTTATTCGATGAGAAATTTAGAAGAAATTTTTCATAATTCAAATTATCAAATAATAATTGTTGAATATGAGCAAAAAAACATTGCTTATTTAATTTATATGAAGAGTGTTGATTTTAATGAAATTTTAAAAATTGGTGTTGATGAAAATTATCGTAATTTAAAAATTGGTAGTTTATTAATTGATTTATTAAAACAAAAAAAGCAAAACATATTATTAGAAGTAAGCGATCTTAATACAAATGCATTAAATTTTTATTTAAAGCATGGTTTTATAAAACAAAACATTCGCAAAAGATATTATGCCAATGGTTCTGATGCTATTTTAATGATTTGAAAATATAATCAAACATAATACAAAAACCCACTCTTTTTTATAAGAGTGGGTTTTTGCATGTAAAAATAAGCCGTGTTCTGTTCTACAAAAATGTAGCGCTAATTATTTATCTATAGAATGTAAACAATTCTATCCTAATTTAATTTCTTAATTTACTAAATTAGATTCCCTTACCGAAATTTAGGTTTCTCACTTGTGGGGTTTACCAACGTTTCATCTCATCAATTTCTTGATTAATCGTCTCTGTGGCACTTTTATAAATTTTTAAACATATAATTTAAATCACTTAGCTTAAAAATTTGCCGTCATATGCCTATGCTATGCCTTATTAATTATACATACACAAACACTACTAACATCACAGTTAGTGTGAGCACGGACTTTCCTCTACATTTTTTAAAAAATGCAGCAATTAGCTTTTTACACCATTAAAGTATAACTTAAAAGTTATGAAAGCTTTAGAAAATTCATCTTATTTTTAATAAATAAATTTAATCTGTTAAAACATTTATTTTTTTTTATTTATTATTTTTGATATAATAAACAAGTTATATTCAAACATGAAATTAAACTTTAGAATTCAAATATTAGGTAGGAGGCGAGTATATGGCAAATATTGTTTCAAATGAAAAAACATACAGACATACGCAAAAAGTACGTAAGGAAAATCATGCTAAAATGTCTAAATTAAGAACAATTGTTAAAAAAACAAGAAGTTCAAATGAACAAGCTCAACTTAATGAAGCTTACAAAGTCATTGATACAACAGCTTCAAAAGGTGTTATTCACAAAAATAAAGCAAATCGTTTAAAATCAAGAACAGCAAAAGCTTTTAAAACAAACTTAGAAGTCACAGCATAAATTAAGAAAATCTTTTGATTCTTTTATAAAAATACTGGGAAATGAAAGTAACTTCACCCCAGTATTTTTATAATAATTTTTTTATATATTTTTTTAAAAGTGCTTTATAATAATAAATTTTTGCAGAATTAATTTTTAAAATTTCACTAATTTCCTTTGTATTATATCCTTTAATAATTAAAATAGTAAAATGATATAAAAATAAATTTTCTTTCTGAATACTTCTTAAAAATTCATAAATTAAAAATCTGTTGTTTAATTCAGCACCAACTTCATCTGAATTGGTACTAATTAATTCATAATTTTCAAAATTATTATTATTTAAATGATTAACTAAATTTTTATTAGATAAAGATTTACGTAATAAATATACTAAATAAAAATAAATTTGCCGTTTAACAAATCCTATAAATTGATTTAAACTTTTAATTCTAGTTACTTGAAATATTTTTAAAAATATTTCATAAATAATAGTTTTTAAATCATTTGCTTCAATCTCTAAAGTATAAAATTTAAAATTCAATTGCTCATTAATATATTTTAAAAAAATTTTATGATAACGTTCATATAATCATCTAATAATCTTTTTGTCGTTAATGGATGCTAAAAAAAATACTTGTTCAAACATTTTATAACCTCTTTTTTTATATAAATAATAACGGTTTTATAAATATATAATTTAATCTTTTTTATTTATAAAATGTTCACAATTTTTTAGATTTTTTTTAGAATTTTTTATTAATGTTTATAATTTATAAACATCATTGTGTATATTTTTTTTAAACATATAAAACATTGATTTTTAATAAAAAAGAAGTATTTTAATAGTGTGCATAACTCTTATTATGTTTATATTTAATAGCATCAAATAAATAAATTGACAATTAAATTAAATAAAAGCATCTAAAATATTGATTAATGAAAAATAGATTTGATTAAAATTCAAAGGGAATATGTAAGGTTTATGGATATAGTAATTAAGAAAAATTGTAATTTTGTTAAAAATGATGACCATTTATATTCTTTTTATACACCACTAATTGGTATGCAAGCAGTAGCTTTATATTCTTGATTCGTTAATAGAGAAAATATTTTTAATAAAAAGGGTACAATTAATTTAACTCATGAATATTTATTAGATGAACTTAATATTTCAATTAATACATACAACGAATTAATTACTAAATTAATTAATGTTGATTTAATTAAAATTTATTCTTTTGAAAATAATTCCCAACAAGTAATTGAAGTTTTTCGACCATTAAAATTTAGCGAATTTATAAATATTGAAAAATTTAATGAAGAATTAAAAAATAATGTTTCATTAAATCATTATAATGTTTTAAATTTATTAAATAAAGAGAATAATATTGATACTAATCGTTTAATTGATTTATCAGCTCAATTTAAGACAAATCATTTTAACAATCTCGAAAATAAATTAGATAAAATTAAAAAAATAATTTTAAAAGAATATGGATTATCACTTCAAATTGATGAATCAACAAAGTTGTGCTTAATAAATTTTTTCAAAAAATATAAATTTAGTATTGATCAAATGTTAAATTTAATCAAAAATAATTTAATTAATAATATTGATTTTTTAACAATTGATTTAAATAAATTAGAATTAACAATTTTTTCAAATATGGAATTCGAAAAAAATAAAACAAAATTTCAATATTACAATATTGAACGTGATTTTAATATTTTTAATAAAAACAGTAATTTAGATCGATTCAAAAATGTAATTAATGATTATAATAATTTTAAACCTTCAGATTTTTTAAAACATATTCGTAAAGAAAAATTAAGTTTAAAAGATAAAGAGGCTATCAATTTATTAACGTATGACAAATCATTAAGTGATCCAATTGTTAATGTTATCTTAGATTTTGTTTTATTTAAAAATTTAGGGCGTTTAAATGTAAAATATTTAAAACGTATTAAAGAAACAGTACTGGGAATCAATATTAAAGATGAGTATGAAATGATTAAATTTTTTCAAAATAAAAAAAATGATCATAAAGAATTAATTTGTGAAGATAGTGATAATGCTAATACTTTCAAAAATACTAATTTTATTGAAGTAAAATTTGGAGATTTATAAAAATGAACAATAATCAAGTTGATGATGAATTAGATTTAGAGAATTTTAATTATCAAAAAGCATTAGAAATTCCGAATATTAAAGCAATTAATTTAACAGAAGAAGAATTTAATTTATACTTTTGAGATATAGTTGAAGTTTATCGTTCTTATTTGAATAATTTAAACAATCAAAATGATAGTTATTACATTTATCAATTAAATCGTAATGAATATAATCACTTATGTTTAGTAGTAGTTAAAAAAGAAAATAAAGTAGATAAAATTAAAAGAAATTATATTTTAAATACGATCAAAAATATGGATTATAATATCTCATTAGCAGATGATAGTCAAATATTTTCTAAAAAAACTGAAATATTAGATGCTAATTTATTAGCAGAACGAAATAAATTAATTAATTTCTTTTTAGAAGAAGCACGAAAAAATAAAAAAAATACGTTAATTAAAAATAATAATCAACAACCAAAATCTGCTTTTATTTATGGTGATTTTAGTGTAGGCAAATCAATAATTACTCAAGCTTATACCAATACTATTTCATTAAAATATAACTTAAAGATAGCTTATATTACTTTAAATGATCTATTTAAAAATGTAATCCAATTTTTTAACTACAAAGATACATCAGATTTAGTAATTAATGAATTAGTTAATCTACTTTCAAATGTTGATGTTTTAGTAATTGATGATTTTTCTAGCGTTAATTTAAATTACTGATCGATTTCAACAATTTTAATGCCTATTATTGAAAATAGATTAAAATCAACAAGACAAACCATTTTTATTTCTAATTTTTCAATTGAACAATTAATTAATTCAACAAAAAATACAACAAATATTGAAGAACAAAAAACTAAATTACGCTTATTTAATCGTATCGAATATCTCACATATGGTAATATTTTTAAAATAACAGGGCCAAGTGTTTTTAAAGTTGTAAATAATTCATAAAAATATTGCTTTTTTATTAAAAATAGTGTATTATTATATCAATGGCTTGTATGGTTATTCGAGCACTTCTAGTTGTTAGATTAGTCTTCAACAATCTAGTTGTAGCAAAAGTCGTTATGTTATAACTATTTAAATAACCAATAAGATCATAAAAAATTGTGATCGAAAAAAAGGAGAACTTCTCCTTTTTTTTTTTTTTTAGAAATACTTATTTGTATTTAAAAATAGTTTTTATTTCTTCGATAATAATTTGTTTTTAATGTCATACCACTTGCAGTCGCGAATCATTTTTATTTCATGCTTATATAAAGGAATTAATTCATCATTAACATTAAACCAAGGCGTTTCTAAAATTTTAGGGATGTTATTTAATAAGGGATGGTAAATAATATTAATTAAATTTGCAAAACCTATCGTTCCATATCCTAAATTTTCGTGGCGGTCTTTGTTTGCACCACAAGGGTTTTTAGAATCGTTAATATGTAATACTTTAATATAATTAATACCAATTTGTGCATCAAATTCTTTTAAATATTGATTAAAATTATAATAATCTAAAATCAAACCGCTATCATTCATATGGCAAGTATCTAAACAAACGCCAATATTTTTTTTAGAATGAACATTGTCTATAATGGTTTTTAATTGTTTTATATTTACCCCAATTTCAGAACCTTTACCAGCCATGGTCTCTAAGCAAACAATTACATTAGTATTAATAGTTGAAAAAATATCATTTAACATTGTATAAACTTGTTCTAAAGCTATATCTTCATTTTGTTCTAAACGTGATCCTGGATGTAAAACAAGTTTAGTGATCCCCATAGATTCTGTTCGTTGGATTTCCTGAATTAAAAATTCTTTAGCAAGTTCACGAACATTCTTTTTAGAAGAACAAGGGTTGATAATATATGGAGCGTGAACAATTAAATCATCCAAATTAATATTATGTTTTTTTAACAAATTATGTGCTTCTTCAATTTTAAATGATTTAATCGGTTGACGTTTTGTATTTTGTGGAGCTCCAGTATAAACCATAAACGTGTTAGAACCATAAGATAATGCTTCTTTTACACTACCATAAAAGAAATCTTTAGCTTTTAAACTTACATGACTACCTATAATTAAATTATATTTATCCATATTATTCATTTTCATCATCCTTAAAATAGAAATTTAATTAATTTACTTACAATTATTTTTTAAACTTAATTGGTTTATATCAATTTAAAACTTCTTCAGTATATTGATGATTTTGTTTATCTTCATAATGTAAATAAAGATTTGGTTCAAAGTGTGTACCTCAACCACTTTTGTAACGCGCTTCTATTAAAACTAAATTTGATTGTTCATAGATTCGTGGATAAATCATTTTAATTCGTTTTGGTTCAAATTGATATTTACGCATAATTTCTAAAAGATCAACTAAACGCGTTGTAGGAATAACCATTGCTAAATTTCCTTTTTGTTTAATTATCTTAGCACACCCTTGCATAATTTGTTCAAAATTAAGCGTGATTTCATATAAAGCTAAAGTTTTTAATGGATTTTTAGTTGATGGAATAATTTTATCTTGTTTATAAAAAGGGGGGTTACAGATTATTGCATCATATTTATTATTTTCGCTTTTATCAAAATCTTTCCAATATTGATTAAAATCAGCATGTATAATATTAATTTGTTTTTCAAAATGATTTTGACTAACATTTAATAATGCAAGGTTAATTGCTTCTATTTGAATTTCAATCGCATCAATTTTTATATCCTCTTTGCGCAAAGCTAAAAAAATACTCAATGCAGCATTATTTGTTCCTATTTCTAAAATTTTTTTCGTCTTAGAAGATAAGCTTATAAAATTTGCTAACAGAATAGTATCAATTGAATAATTGAACATTTTTTTATCTTGATATATAAATAAATTTGTATCAAAGCCTAAAGTATTTTTAATGTATTTTTTTTGCATAAGTAGTTCTTAATTAAAGAAAAACTCCACTAATAGGGTGGAGTTTAGTTAAATATTATTAATGGAGCGGGTGATGGGAATCGAACCCACACTAATAGCTTGGAAGGCTATAGTTCTACCATTGAACTACACCCGCAAATGGTGCTGGAAGAGGGACTTGAACCCTCACGACCGTGAAGTCACTGGATTTTAAGTCCAGTGCGTCTACCATTCCGCCACTCCAGCAAAAATAAAAAAGTAATACAATGTATAATTGTTAACCATGCATTACTTTAAAATAATAAATGGTGCCCCATAAAGGTTTCGAACCTTTGACCCCTTCATTAAAAGTGAAATGCTCTACCACTGAGCTAATGAGGCATAAACACACAATAATTATTATAACATAATAACGAAAATAATTGTGGTTAAAAAATAAATAATTTAATGGCTGGCCTGGTAGGGATCGAACCTACGCATGGAAGAATCAAAATCTTCTGCCTTACCTCTTGGCTACAGGCCAATAAATGGTGGACAGAGTAGGATTCGAACCTACGAACCTCTCGGAGCCGGGTTACAGCCGGATGCGTTTGGCCACTTCGCTATCTGTCCAATTTATATAGTGTGTTTAATAAAACACTATAAAATTATAGTAAAATTATTATATAATGTCAATCTTTTTTTAGTTAAATTAATCAATAATTTTTATAATTTTTACTTTATAAGGTGATGCTAAAGTATGAACTGTAACTTCAGTACCTTTTTTTTGTTTTACAAGAGCTTTTCCAAGTGGTGTGTCTATTGAAATTTTGTTTGCAAAAGGATCTGAATCCATTGAACCAACTAGTGTAATTACTTCTTCACAATCTTTTTGATAATTATAAATTTCAATTGTCGATCCAATGGAAACTTCGTCAGTTGAACGTAAAACATCTTCAATTAATTCATAATTTTCTAAAATTTCTTCAATTTCATCTTTACGTTTTTTTAATGCTGCTTGTTCATTTTTTGCAGCATCATAATCAGCATTTTCAGATAAATCACCTTGTTCGCGTGCATCTTGTAATTGCTTTGTAATTGCTGGTCACTTAACATCTAAAATTTCTCTTAATTCTAATTGTAATTCTTCAAGACGGTGTTTTGATATCGTATACTTTGCCATAGTGTTGCCCCATATAAATAAAAATATATTATTTAATTATATCATTACATAATATATAAAAATTAATAATAAATAAAAAAACCAACCTCTTAAATTTCAAGGTTGGTTCTAGCGTAGCGATGACCTATTTTACTTGCGCTATTTTCGGCTCTAGAGTGCTTGACTTCTGTGTTCGGGATGGGAACAGGTATTTCCACTCTGATATAATCACTACACAATTATGATCGACATCTGAAAACTAAATATTATCAACTATTCTTCTAAAAATTAACTAAGAACTTATATAAGTCCTCGAATTATTAGTATCGGTTAGCTAAACACGTCACCGTGCGTACACATCCGACCTATCAACGTTATAGTCTTTAACGATTCTTACTTCATTTAAGAAAGGGAAGATTAATCTTGAAGTTAGTTTCACACTTAGATGCTTTCAGCGTTTCTCTATTCCACACGTTGCTACCCTGCAGTGCCGCTGGCGCGACAACAGGATCACAAGAGGTGTGTCCATTCCGGTCCTCTCGTACTAGGAACAGCTCTTCTCAATCTTCCTACGGGCATGACAGATAGGGACCAACCTGTCTCACGACGGTTTGAACCCAACTCACGTATCTCTTTAATCGGCGAACAGCCGAACCCTTGGAACCTGCTTCAGCTCCAGGATGAGATGAGTCGACATCGAGGTGCCAAACAGTGCCGTCGATATGAACTCTTGGGCACTATCAGCCTGTTATCCCCAGAGTAGCTTTTATCCGTTGAGCGATGGCCATTCCATTCTGAGCCACCGGATCACTATGACCTAGTTTCCTACCTGTTCGACTTGTAAGTCTCACAGTCAAGCACCCTTATACCATTACACTCTATACACGATTTCCAACCGTGCTGAGGGTACCGTTGCGCGCCTCCGTTACCTTTTGGGAGGCGACCGCCCCAGTCAAACTGCCCACCTAACACTGTCCCCCAGCCAGATTCATGGCTGCAGGTTAGAGAAATCTTATCACAAGGGTAGTATTCCAACGTTGACTCCATATACCCTAGCGAGTATACTTCACAGTCTCCTACCTATGCTCTACAAGTAATAAAATTTCCCAATATTAAGCTACAGTAAAGCTTCATGGGGTCTTTCCGTCCAATCACGCCAAGAGGGCGTCTTCACCCTCACCAGGATTTCACCGAGTCTACAGTTGAGACAGTCAAGAGATGGTTACACCATTCAAGCGGGACGGAATTTACCCGACAAGGAATTTCGCTACCTTAGGACCGTTATAGTTACGGCCGCCATTCACTGGGGCTTCAGTTAAAAGCTTCGCTTGCGCTAACATTCTTCTTTAACCTTACAGCATTGGGCAGGTGTCACCCCATATACATCGTCTTACGACTTAGCATAGAGCTGTGTTTTTGTTAAACAGTCCCCCCTCGCTATTCACTGCGGCTCACCTTTTACAGCGAGCACCCCTTATTGCTAACGTACGGGGTTAATTTGCAGAGTTCCTTAACTGTAGTTAACTCGCTAACCTTAGGATATTCTCCTTGACCACGTGTGTTCGTTCTCGGTACGGACTACCAATTGTTTAACGCTAGAAGCTTTTCTTGGAAGCATGATGTATATGTCTTCGCTACTGCCCGAAGGGGTCGCTCGTATTCATAACTTGGTCCTAATAGTAAGCGGATTTGCCAACTTACTAACCTTGTTATTTAAACCGGAATCCAATAACCGGCACATAATAACCTTCTCCGTCACTCCATCACACAATTTGTAGGTACAGGAATATTAACCTGTTTTCCATCGACTACGCTTTTCAGCCTCGCCTTAGGATCCGACTTACCCTGGGAGGACGACCCTTGCCCAGGAACCCTTAGTCAATCGGTTTGAGAGATTCTCACTCTCATTCGTTACTCACGCCAGCATTCTTACTTGTATAAAGTCCAGTAGTCTTCACAGTCTACCTTCGCCCCATATACAACACTCGTCTACCGCTACATATATAATATGTAACCCATAACTTCGGTTATATGCTTAGCCCCGTTACATCTTCCGCGCAGAGTCTCTCGACAAGTGAGCTGTTACGCACTCTTTAAACGGTGGCTGCTTCTAAGCCAACATCCTTGCTGTTTATGAAACTCAACATCGTTTTCCACTTAGCATATATTAGGGACCTTAGTTAATGATCTGGGCTGTTTCCCTCGCGACAATGAAGCTTATCCCCCACTGTCTGACTGCTAGAATAGGTAATATGGTATTCAGAGTTTGATTCTATTCAGTACACCGAGGTGGCGCCATCATAGATTCAGTGCTTTACCCCCATATTCCCAATAATCTAACGCTATTCCTAAAAATATTTCGACGAGAACGAGCTATCACGGAGTTCGATTGGAATTTCACCACTATCCACAGGTCATCCCCTGTCGTTTCAACGAAAGTGGGTTCGGTCCTCCAGTGAATGTTACTTCACCTTCAACCTGCCCATGGATAGATCACCCCGTTTCGCGTCTATTATAACATACTAAAACGCCCTATTAAGACTCGGTTTCCCTACGGCTCCATCTCTACGATTTAACCTCGCATGCTATAATAACTCGCTGGCTCATACTTCAAAAGGCACGCTATCACACATTAATGTGCTCTAACACCTTGTAAGCTTATGGTTTCAGGTTCTATTTCACTCCCATCTCTGGGTTCTTTTCACCGTTCCCTCACGGTACTATGCTCTATCGGTGACAAACTAGTATTTAGGCTTACCCAATGGTCTGGGTGGATTCAGACAGGATTTCACGTGTCCCGCCCTACTCAGGATCCTACTAGGTAAAAGAATAATTTCGTATACGGGATTATCACCCTCTATGATTTGGCTTCCCAACCAATTCTACTATCATTCTCTATTCCATATTGTAGTCCTACAACCCCGAATCGAAATTCGGTTTGGCCTGCTCCCCTTTCGCTCGCCGCTACACAGGGAATCACTTCGTTTTCTTTTCCTCTGGTTACTGAGATGTTTCACTTCACCAGGTATCGCTCTGTTAAACTATGAATTCATTTATACAGTAATCTAATTCTCATTAGATTGAGTTTCCTCATTCGGAGATCAACGGATTAAAGCCTCTTATCAGCTACCCGTTGCTTATCGCAGATTAGCACGTCCTTCATCGCCTGTTTGTCCCAAGGCATTCACCATAAGCTCTTAGTAACTTATTATAAATCCTTAAAGTTGTTTATTTATGATTGTTAACTATATTTAATTTAATATAGTTTCTGTTCACTGACAACTTAATGTCAATGATTAGAAAGATTTGTTGATAATTATTCAGTTTTCAAAGATCGATCATCCATAATAAATTAATTATTATGGGAGAGAATAAACTCTCAAAACTAAATAGAATCCGACCATATGAATTTTTAAATATTTTTAAAAAAGTGGCTTATATTCATATGGATTTTAATAAAAATGTACACTTATTAGTACGTAAATTAAAAATTTACTCCGAAGAAAGGAGGTGATCCATCCCCACGTTCTCGTAGGGATACCTTGTTACGACTTAACTCCAGTCACCGATCCTACCCTAGACGCATGCCTCCAAAAGGTTAGCTTTGCGGTTTTAGATATTACCAGCTCCCATAGTTTGACGGGCGGTGTGTACAAGACCCGAGAACGTATTCACCGCGACATGTCTGATTCGCGATTACTAGTGATTCCAACTTCAAGAGGACGAATTGCAGCCCTCTATCCGAACTGAGACTAACTTTTTCTGTTTCGCTTCATCTTACGATTTTGCAGCAGTTTGTATTAGCCATTGTAGCACGTTTGCAGCCCTAGATATAAGGGGCATGATGATTTGACGTCATCCCCACCTTCCTCTACCTTGCGGTAGCAGTATCGCTAGAAAAGTAACTAACGAAAGGGGTTGCGCTCGTTGCGGGACTTAACCCAACATCTCACGACACGAGCTGACGACAACCATGCACCACCTGTCATATTGTTAACCTCAACTATATTTCTATAGCATCGCAATAGATGTCAAACCTAGGTAAGGTTCTACGTGTATTGTCAAATTAAGCAACATGCTCCACCACTTGTGCGGGTCCCCGTCAATTCCGTTTGAGTTTCATTCTTGCGAATGTACTACCCAGGCACATCATTTAATGCGTTAGCTACAACACCGACCCATTCGGGCCGACATTTAATGATGATCGTTTACGGTGTGGACTACTAGGGTATCTAATCCTATTTGCTCCCCACACTTTCGAGCCTAAGCGTCAGTGATAGTCCAAGTTGGCGCCTTCGCCACCGGTGTTCTTCCATATATCTACGCATTTTACCGCTCCACATGGAGTTCCCCAACTCCCTACTACACTCTAGGTTTACAGTTTTTGATACAGCTAGACGTTAAGCATCTAGATTTAATACCAAACTTACAAACCCGCCTGCGCTCGTTTTACGCCCAGTAAATCCGGATAACGCTTGCATCCTATGTATTACCGCGGCTGCTGGCACATAGTTAGCCGATACTTATTCAAATGGTACAGTCAAACTAAAATCATTTCCTATTTTAGCGTTTCTTCCCATATAAAAGAACTTTACAATCTATAAGACCTTCATCGTTCACGCGGCATTGCTTCATAAGGCTTGCGCCCATTGTGAAAAATTCCCTACTGCTGCCTCCCGTAGGAGTATGGGCCGTGTCTCAGTCCCATTGTGGCTGTTCTACCTCTCAGTACAGCTACGCGTCATTGACTTGGTGAGCCATTACCTCACCAACTATCTGATACGTCGCACCCTCATCCAAAAGCGTCGCCACAAAGAGCGACTTTCTACATCTTCTCATGCGATATTGATGTTATTATTCGGTATTAGCTAATCTTTCGACTAGTTATCCCCAACTTAAGGGTAGATTGGATACGTGTTACTCACCCGTTCACCACTAAGCCTAAAAGGCTTCGTTCGATTTGCATGTATTAGGCATGCCGCCAGCGTTAATCCTGAGCCAGGATCAAACTCTTTAAAATTGACGGATTCTATTTAGTTTTCAAAGAACATTCTTATTGTGATTAATTATTAATAAGCTTTAAACACAATACATTAAAGTATAAACAACTTTTTTTAAAATGTCAAGAAATATTTTATTTTTTTTAAAATTGTTTAACTATATACTTAAAAAGTACGTAAAAGATTATAATCAAAAAAATAATAAATATCAAGTTTTTTGTTTAAAAATAAAACAAAAAAGTGGTTTAGACCACTTTTGAGATTTTATTAAGATTAAATTAATTTAGAAATTTCCAGAAGTTACTAGATGATCATTATGTTCAGCTTGTTTCTCAAAAACAATTCGTGTTGCTTGTTCACGATATTTAACTTTTTGTTCAATATTTAGAGAATTAACTACAACCTGATCTTTTTGCATCAAAATTTGTTCTTGGTCATTAATCGCTAAAAAGACTATACTTGATAAATTATTTTTTTGATATAAAACTAATTTATCATACTCATTTAATAAATTTAAATCCTGATCAAAATATACACCATTTGATAATTGATTTTTTTGTTCTTCTTTTTGTTTTTGAACTTCTAAAGTGGCTTTTTGTGTTTGTTCTAAGGCAATTTTTAATGAAAAATAATTTACAAAACCAATGATGCTAACAGCAAAAGCAATTATAATAGTTAAAAATGCAATATAAACACTTTTTTTTGCATAAAAACTTGGTTTTAATTCTTTTCAATATGTTAAAAAAGGGTCTCTATCTGTCGCATAAAAATCTCCTTCTTGCAACGTAACATTAAAATTATTAGGACGAAAGTTGATACCAATAGCAATGTAACTAAGAATTCAACTAATAATAATTAAGGATCCTACTAAATAAAGAGTGGTAATTGAAGTGTGATAATAAAATTTTTCACTAATAGGAAAATCAGCTTTAGATCGTGATTTTTTATTTAAAAAATAATATAAAAATAATAAAATAAATGATAAAATAACAAAAACAATTGGAACCACATAACCAGCAATATTATATTTACTAAACCCTTCTAATTGTTCTAAAAAAATGCCATTGTTTGGGTTATATAATCTTTTAACTTCATTACCATCAATTTTGTTTTGAGTTAACGTATCAAAATTTTTATTAGGGCTAAATCAAACGGCAAAACCAACGACAAGACTAACAATACATAAAATACCAATTAAAACAATAATATAATTTATTAATCGTTGCTTCATACTTTTTTTCCTTTCTTAAATAAAACTAAATAAGCTTATTAACAAGATTTGCAAGTAAATAAAGACTTGCAGTTTCTGATCTTAAAATAGTTTTTGTCAAACTAATACAAATAGCATTATCGAATTGTTTTAATAAATTAATTTCCATTTGTTCAAAACCACCTTCTGGTCCAATCACTAAAGCAATTTTTTGATAGGTCAATTCTTCTTTAAAAGCATTATTTAAAAGATTTTGTTTTTCATTTTCATATGCTATTAAAATTAAATCATAATCTCTTAAATATTTTAATAATTCATTAAAACTAAATTGAGCGTAATAATTAACAAGATGATTACGTCGTGCTTGTTCGCACGCTTCTTTAATAATTGTTAAAATACGATTCGTTTTAATTAGTGAATGATTATAAGTTCTTTTAAAAATTGTTTGATAAATACTATTAACACCTAATTCACATGCTTTTTGAACAATTCACTCAAAATTATTTGGTTTAATGCTTGCTTGAAATAAATCAATTTTAGTAGCTAATTCACAATTAAGAGTATATGGTTTAATAATTGTTGCATGTAATGGTTCAAGATTAATAATCTCAGCAACAAATGCTTTTGTTTTATTAATAACAATAACTTTATCATGTAATCTCATACGCATAACATTCTTGATATGATGAACATCAGAATCATAAAAATAAACTTTTTTGTCTTTAATTTTAGAAACAAAGTAACGTTGCATAATTTTTAATTTAATAATTGATCTAAAATTGCATTAATAAACTTATATGATTCGTTGTTAGAATAATTATGTGTTGTAATTAAAGTTTGATCAATAATAATTTTCTTAGGAGTTTTTAAAACCAGATATTCACCATATGCGCTTAGCAACAAAGCTTGCTCAACATATGATAAACGTTCAAATGTTCAATCTTTACTAATAAGAGGTTTAATCATTTTTGTTAATTGTTCATAATTATCCAAAATATATTCACCAACATTTATTCAATTAAGATCTAAATCAAAGTCAACTAAAGCACATTTTAATGCTATCGGTTTATTTTTTTTTGTAATTAATCAATGATAGATAAATTGAAATAATTTAACACGTTTAGCTCATTGAGTTTTCATTTTTATAAATCAATCCCTATATTTACACTTTTAGTATCAACGCCTAAATTTGAATAAATAAAAGAAGCTAAATGATTTTGTACATCTCTTACTTCATCAAGATCAACTTTTTGATGAATATCAAATAATGAAATAATAATAGATATATCAACAAGGGATTCATCATACTGAACAATATCGATATTCTTAATTACAAATTTTTTATTAACAATCAAATTAAATTTTTGTTCAATAAACTTTTTAATATTTGATTTTGAGATTAAAATCTTCCCTGATTTTGTATTATTAATGGTAATGTACTGCATTATTATTTAGCTCTTCCTACATATTGACCATTTGATGGATTAATAATCACTTTTTCACCAGATTTAATAAATTGTGGAACTTGAAACTCTCATTTTGATTCTAATCATGCACGTTTTGTAGCATTTTGAACTGAATTACCCTGAACCGCTTCTTCTGCTTCAATAATTGTTACGACTAATTGATCTGGTAAACTTACACCCATTAATTCACCATCATAACGCATAACACTTACTTCAGTACCTTCCTCAATAAAATTTTTCTCTCAACTTAATTGACTGTAAGGAATTGAAATTTGTTCATATGTATCATTATCCATAAACACATAACCACTACCATCATCATAACTAAAAGTCATTTTTGATTTTTCAATCACTGCTTGTTCAAGTTTTTCTCCAGTTAAAACTTCAATAGTGATTGCACCAGTACGTAAATTTTTAACTTTACATTTTACAATTCCTTCACGCATAGCTGTTTTATTAAACGAATTTTCTATTACTTGGTAAATATTACCTTTATATAAAAAAGTGTGGCCAGCACGTAAATCTTTTGCTTGAATAATTGTTGCCATTATTATTACCTCATATTTCTACAAAAATAATATAAAATTATAACCTAATTATTAGCATTATTATGTATTTAAAAGGTTATTATGTTTTATATTCATACAAAATAATTATTTTTCCCTAATTATATTAATATAGGAGTTAGAGATGAATAATAAAAACAATGGAATGCATCTTGAAGTATTAATTAATAAATCAATTAGCTTGTTTAATTTACAATATCAAGCTTTTTTTAAAAAAAGGTGTCTTGATATTATTATTAAAAATATTGATAATTCTTATGTTCAAGGAAAAATCAAGCAAAAAAGTGAAACTGATTATTACGGATTCTATAAAGGTGATTATTTTGATTTTGAAGCTAAACAAACAAATAAAAACTCATTTTTAATAAAACAAATACAACCGCACCAACTAGCACATTTGTATTTAATTCATAAAAATTCAGGTTTCTCTTTTTTAATTATTTGTTTCATTAATTACAATCTATATTTTATTATCACTTTTCAACAATTAATTAACTATTATCAAAAAACTAAGCGGAAATCCATTCCCTTTCAATGATTTAAGGATCAATGTATTGAATTAGAAATAATTTTTCCAGGTGTTATTAATTTTAAAAAAATGATTAATGATTTGAAATTAAAATATTATGATGATTAAATAGGTTTATTATTCTTTCGAACATAGTTTTTGTATAGAAATAAATCCTTTGCCGTTTCAATACTATAAATTTTAATTTGTTTAAGTTTTATGTTATATTTTTTATTAATACTAAAAAATTTAACTTCTGTTTTAGTAGTACTTAGAGCTGAATCTGTATAAAAATTTTTATTATCATACTCATTATTAAAAATCGATTTAGTGTTTAAAAATGGTAAATATAAATTATTGTAATCAAAACTCAAAATTGAAGTTAAAAAATATTTTGAGTCTAATGAATTATAAGAAACATATAACAGATCGTTATTTTTGAAAAAATTTTCATTATATTTCTCTAATAAACTAACCTTTATTTTTTCATAAATCAAAGCACTATTATATTTATCAAAATTCAAATCGTTTTTTATTAATTTTTCAAAATCATGATAATTGTTAATCAAATTATAATTATATAAGTCATTAGATATACTTGATTTCTGAGGAATATAAAAATCTGAATAAGATGAAACAAAATTAGTATCAATTGTTTTTAATTCTCGTATACTTTTAGTAATAAATGAGTATTGTTTATTATATGATATCTTATAAATTAAAGGTGTTATTATAGTAACTGATAAACTAGAATATCCAAGCACACGAAATAAAATCTTTTTATTCTTTTGATTTAATTTTCAAAAATTAGTTTTTACATCAATATTTGACATTATAATTCTTCTTCGTAATTCGTAATTACTCATGAAATATCATCATCGTTTTCACAAGCATTTACAAAACGTACTAAACGTTCGCGAGCATATTCGTCTAAACTAATGAAATCATTAGGAATAAGTTTGATTTCCGCACTTTTTATATCAGCATTATTTTTTTCAAGAATTAATTTTACTGTGCTAAAATCTTCTGGCTTAACTAATATTTCAATAATTTTATCATCTTCATTTATATCTATAATACTTTGATCAATAAGAATTTCAAATCATTCATCTAAAGATTTAGTTTCTTTATTAATTAATAACTGTCCACATTCATTAAACAACATACTAACAGAATTAGGTTTAGCAATCTGACCTTGTAATTTCGAAAAATAACCACGAATAGCTGATATTGTCCGCTGCTCATTATCAGTTAAAGCACGAACAATAATTGCTAATCCATTTGGTCCATATCCTTCGTATGTTAATTCTTTTAAATTATCAATATCTTTACTGGCACCATTAATGTTTCGTTCAATTGAATCACGACTCAAATTATTATTTAAAGCGCGTTCAATAGCAACTTTTAAACGCGAATTTGCCTCTGGGTTAGGACCACCCATTTTTGCGGCAGCTTTAATTTCTTTTGCACATTTCATTCAAATTTTAGCTTGTTGTTGTTGTTTCTTATTAATTCCACTAGCGATTAAATGTTTTCTTGGCATATCTTTCTCCTATTTTAGTGCATCTTTTAATAAAAAATGATTTAAAAAATCAATATCGATTTTTATACCTTCATTATAATATTGATATAAAAATATATTAATCATATTTTCATAATATCTTTTTCATTCATTATTGACTAATAAAGATTCATATTCACTATGATTAATATTTAATGATTCATTTTTAACAATCATATCATATAAAATTTTCGAACGAGTAGCAATTTTATTTTCATTTTTTTTATAATCAATAAAATTTTTGGTCATAAAATCAGTTGGCCTGATATAAGTTTTTGTTTGATCATTATAAATAATTGTTCAAGTAAATCCTATATCTAATAAATTTTCTGTAATAACAGATTTAATAGTTTTTTTAGAAATAAAATAATTTTTTTTATTAGCATTGACAAATTGATAATTATGGAAATAAAATGTTTTCGTATAAATAGTTAGATTTTTATCTTTAAAATCATATAAGGTACTAATATCAGCATCATTCACAACTTTAATTATTTTATTCAATGTTTTTTTTTCGTTTTGAATTTCATATCTTAATTTTTGAATAAAACGCAGACGAGAAATATATCCAGCATAAAATTTTGCTTTTTGAATCATTTCATTCGTTAAACTAATTTTTTTATTTTTATTAATAGACTCAATAATTTTGTGTTGATTATCAATATAATCATTAGCTAATTTTGAAATATCATTAGTAATTGAAGAAACATTTGATTTTAAAGGAATCGAATCAGGAATAATAATAGAACAAGAACTTGCTAAAATAGGAATTATTCCTAACCCTAAAAAAGTGCTTATTTTTATTATTATTTTTTTAATATTAATTTGCATGAATTATATTTACTTAAATCATTTTTTATTCTTATTGACTAATATTATTATATTTTAACATTTAACAAAATAAATTAATAGATACAATATCATCAAAAATTATTTTTATAAAATAAAAAAATATTAAAGATTGTTTAAAATCTCTAATATTTTTATAATTATTTCATTGTTTTAACTTTTTCACCATAACCGCGTTTTAAAGCTTTGAAAATATAAGTTGCTTTATTTTTCTCTTTAGAATCGTTAAAACGTGAATTTGTTCTTCATTTTCAATATGTAATCATATATAAAAAATCATTTTTATTATAATTCCACTCTTTTGGTGTATTTCAAAGCATTGCACCACGATTCCCCATAATCATTGAGCGTACTCGTTTTTCACTTTCAGATCCCATATCTGCTTTTAGCAATAAATAGACACGTCCTTCACCCGATAATGAATCAGAACCAGTCCATCATAGCCCACCTGAAACATTTCTAAAATATGTTTCATGAACATATCATTGCATCACAATTCCTAAATGAATTAGCGAACGCATATATGGATTATCTTGTTCATATTCTAAATTAGGATGATTTTTAGCGTATTCTGCACGAGCTGATCATAATTTATTAATTAAATGTTGGATAGCATCTAACATATTAATTTCACTAACTTTTGCTGCTTCTCCTGTTTTCTTTTTGCCACTAGCAAATAAAACAGCATTTAAACTATCTTCTTTAAGATTTTTTAAACCATAATATAAAGGATTTTTATCTTGTGTTATGCTTTCGAAATCTTCAGCTAATGGTGATTTTTCAAATACTAAATTAATTAGATCACCTATGGTTTGGGGTTTAATTTTATTTGGTTCGACAAGTTTTGCAATTTTTAATAAATTAGTTGTTTCAGTTAAACCAAACGTTTTCATAGCTTCATTTACTTTTTTTATACCCTCTTCTTTTGTAAATAAATCAAGTCCATTTTCTTTTATTAAAGTAAATATTTTATCAATGAATAAATTTACAAAATCTAATTGATCAATTTGATTAATTAATTCTTTTACAAATGTTTGAACAATTTTTACATCTTGATCATTAGTATTTTCTAATTTTAAAGTTTTAAAAAGGTATTGAGCCATAAATTGTGTTGAAGAATCAATAACTAAATAACGTTTTATAAAAGGTACAAAATCAACTTTTTGTTGATCAACATTTCGTTTAATTAACATATTTAAAATAATATTAATTGATGGTTGATTATTGTTTGTAATTTCTTTTAAATATTCATCTTTATGATCAACTAAATCATTAACTACTTCATCAATAATCTTGATTAATTTTTCATCACTTATAATAAATTTATATGATTCTTTTAAAAATGATTCACTATATTGTTTATCCACACCACTAAGATTTGAAAGTTTTTGTGCGAAAGTATTACTAATCAACTCACCAATATAATTATTTAATTGTGATGATTTAGATATAGTTACTAAAAGATCTTTAAAAATATGTTTAATTTTTACAATTGTATCTTGTTTTTTTAAGTAAGAAGAATTTAAAATAGTTTTTACATAATCTAATATTGTATATTTTTGAATTACACTTCTATTAGTTTTTTGATTTGTGTTCTTTGTTATAAGATTAATTTTATTACCAAATAAAATAGTATATGCGTTTTGAGGCAAATGAGATAAAATAACTTTTAAGAAATTTAAAATGTCATCATTTTTATCCAAATCAACAATTTGAAGTAATTTTGCAATTTGTTGCGGAGATGTTAAATATGAAATAACATCATTTTGCATAACAGTTACAAAATTAGTGTTTTGATCAACAATTTTTATGGCGTTTGAACTTAAAGTCGTAATTAATTTAACTAAACCATCATTAACATATTCAAGATTGAAAATATTTTTGATAACAAAATTAATAAATGACTTTAGTTTATTGATATCTTCTACTGTTAAATTTTCTAATTTAAAATATGTTTTAATTGTATTAGCAAATGATGTTGAAATTTCATCAATTAATGAAGATGAACCACTGATTATTTTATTAATTGCTTCTTTTAATTTATCAATAATTTGTGTTTGATTCTTATTAATGTAAGTTGTTAATAATTCACCAAAAGTTTTACAATTTTGATATTCATTTTTATGATCAATCAAACTATTGATTGCATAAGTGATCAAATTTTGAACTTGCTCAAACAAGTTTTGATTAGTAAATACTTTTTGTACTAGTGTTTTATAGTTTTGTTTTAAATTAGTTAACCCAGGGAATGTATTAATAATTAAATTACTTAGTTTTTCACCTAATCCATTGCCCAAATTGTACAACAAGCCCTTATTATTATTTTCAACAATTTTTTTTGTTAGTTCATCAATAATTGTTTTTAATTTTTCTTTTGATGTAGAATCCATTTCATGCGCAAATAATGTTTTTACATAAATATAGTACTTATTTATATCTAATTTACTATCATTATCATTTTCATGTTTATTCAATGGATAATTTGAAAGATTACTGTTTTTGACAGCTTGATTAAAGAAATGATTAAAATCAATTTCATCTAAAATTGATTTAATAACTTCAATATAATCATTACTTGTAATCTCGTTGACTTTTAATACATCAATTAGTGAAATAATTTGTTTAGGATCACTAAAATCAAAAATCGTTTTAATAAAATTAATTACACTATCTTGCTGTTCACTCAATAATTTTGTTAAATTTGTTTGATTTGTAATTGAACTAAATAAATTATCGAGTAATTTATTATATAAATCAAATTTTGTCAGATTGTCACCAAGCTTCTTAATAAAAGTGACCACTTTGTCTTTATTTATTTGCTGAATTTGATCATATTTTTTTTGTGGAGAAATTTGTTTAAATATTACACGCGCTAACAATTCATCAATTTTATCGTTTTGAAGTATTTTTTGAATAAAATTATTTAAATTACTTTTTAAATTAGTTTGCTCAAAACTAATAAGTTTAACAATTAATTCTTCAAAAGAATTTGTGTTCGCTAATTCACTAGCATGTAAAATAAAGCTATTTGTTATGGTTTTTAAAATTGTTTTTAAATCATTACTACTAATAATAAATTCAACTACTGGTTTAATTAAACTATTTTTTTGATCTTCGTCAACATTTAAAATTTTTGCTAAATTCGAACTGAAAATCCAATTTGATAATGTATTACTAATTAAAGTTTTAATTTCTTGATTAGCAAATAAAGAATCAATTAATGTTTTAATTGTTTCGTTAATTTTTACAACACTAGTCTTGGCATGGCTTTTATTTAAGGTTTGTGTTTGTAAAACTTTTTTAATAAGTAGATAAATTTTATCAATTTGTTTTTTAATATCTTGTTTTTGTGCTTTATTGTTAATTATTGAATTTGATGTTATGTTTCTAATGATTTCTTCAATTTTATCAATAGGTGCATGATTAATTAAAATCGTAATTAATTGGTTTCATTCATTATTTGTAATTTCATTAGTTTCAAAAATTTCTAATAAGTTACTTAAAGCACTAATATCTGTAACATTTTTGTTAATAAAATCGTTTCATGTTGTGTTTTTAAGTGGTTGATGATTAATTAATGCTTCAATATTTACTTTTAAAAAATCTACTGATTGATCAACAATATTTTGTAAATATAATAAATTAGGAATTTGCTTTAAAATTTTGTCAAAGATATTAATTAAATTTCTTTTATCTTCTTCTGTTAGATTTTTAATTTCAAAATAATTTAAATACGAATTAATAATAGTAGTTGATGTAGCTTTTGTTAAATTTATGTCTTTTGTAATTGCACTTAGGAGTTGTTTTAATTGTTTTTTAATTCATACATCATCTATATTGATAACTTTTTGAATTAAATCTCCAAAATTTTGTGAGTTTTTTAATTGTTGTTGATGATCAATTATTTTTGCAAAGCCTTCAACAATTAAATTTTGGACAACATTTTTAAAATCAATACCATTTAATAAATTGTTAACTAATTGTTGATATTCATTTTTCTGTGGAGTTAATACTTGAATTTTTTTAATTAATAAATCACTTAATTTATGACTAATAAACGATGTCATTTTTTTTCATAAATGGTTATGATGGTCATCATTCATTACATCTTTAACGAATAATTTGACGATTTCTTTTAATTTTGTTTTAGTTAACTCACTAAAATTATGTTCAACTAAAGCTTTTAAATATTCAAAGTAAATATTAATATCGTATTTTTGATCATTGGTAATATTTTGTTTTGTTTGTTGATTATTAAAACCAATATCATTAAAGATAACATTCAAAACATTTACATAATCTTGGGCAGTAATGTCTTTGATACTAAAAATATCAATAACTTTTATTAGTTGTGATGGTTGATTTATATTAAAAATTTCTTTTAAAATACTTCCAATTGATAAAAAATCATCATTTAATAACTTATTTAAAATAGATTTATCGCTTAATTTTGTAAATAAACTATCTAAAAGATCATTATACATACTAAACTTATCTAAATTATTTGCTAACTTAGTAATTAAAATTTTGATTTTATTAATAGCTGAATTATTAATACTATCGTATGTTTTTGTTGTATTAATTTGTGGAATAATATAACGTAATAATAATGTTTGTATTTCATCATCTAATAATAAATTTTTAATAATTTTAGTAATAGCTGGCTTAATTTTCTCTCCACTTTTAATAATAATCTGACTAATAAATTCTGATAAAGTTTTAACATTATCAAAATTATTGTTATTTGATAAAATAGGTTCAATTAAGGCGTTAAAAATTGTTTGTAATTGTTTATCTTTTAAAAGAGAATTAATTACATGTTCAATAAGTTGTGTTTGCTTTTTATCTTGAACATTAATTGATTTCAAAAAAGATCTAATGGGACTTAAATTTGATAATAGATTATTAATAAAATTTTTAATATTATTTGATGTTAAAAGCTTATTAAAAGTAAATTCGATAATGTTTTTTGCTTTAGTTAAGGTATTATTATCAAATGTTTTAGCTTTTTGTGATAATGCTTTAAATAAATCTAAAATGATGTTTTCAATTTTATTTAATTCTGGTTTTGTTTTTGAAATTGAAATATTTCTAACAGATAATAAAGAATTAGTAGGCAAAATTTTAGAGTTTATTAAATTATTTATTGGCAAATAATCAATAAAGAATGTAATTGCGCTTTTTCATTCTTGTTCGCTAATTTCATTATATTTAAATAAGTCTAAAAGAGATGCAAAATCACTTATTTTAGGTATGATAGCTTGGAATAAACTACTTTTAAATTTATTAAAATTCAATGTTTTTGTGTCAGAACTTTTTGTATATTCACTAAAAAAATTAAAAAAATTATCCACTAAATTAGTATAAATTGGCAATTCTGTAATGTGAGCAAAAATTTTATTAATAAATGAAATCGTATTTTGTTCAAGATCACTATCTAGAATACTTATTTTGTTTTCTAGTTTAAATGATTCTATTATTACTTGTCCAATATTAGTGATTAGTGTTTGGTTTTTTAAAATTGTATTTAGTAAATCTTTTAATTGTGTTTTTAGATCGCTAGCTTTATTTTTAAAGAATACGGAGATTAATTCACTAAACGAATTAATGTCTTTATATTTATCTTTATGATCAATCAGTTCATTTAAAATTGTTGATAATAAAGTTTTGGCTTTTTGTAAAAAATCTTGATTATTTAAAATCGATGAAAAAATCTTTGTATATTTATGTTTAATTGTTAATGATTTAGGTAAAACTGCGGTAATTTTATCAACTAAAACATGTGATAATTGTTCTAATTGAATTCTTAAAAAACTACTTGAATCATGAATTTTAACAATATCATCTAATAAATAATTAACAACATTTTTAATTTTTTGTAATTGTTGATCACTAACACCATTATTTAAAGCTTTTTTCAAAATTTGAAAAGCAAAATCATTTGTTTTTTTATCAATTGTTTTCAAATTATTATTAACATCATTATTTTGAATACTTGTAACATTAAAACCAATTTCAACTAAAATAATTTCAATAATATCAGCATAATCACGAGCATCAATTTCATTAATATCTAATAAACTACTAATTTTCAAAACTTTTTCTGGTGTGTCAATGTTAAATGCTTCTAGTAGTGTTTTATTTATTACACTCACATCATTTGATAATAATTGTTGCATTTTTGTTTTGTTAGAAAAAACTGTAAATATTGAATTTTTTAATTGTTGATATAAATCCAATTTACTAATTCCTGTAGCAAATTTTGATATAAATGTTTTCAATTTATTAATTGAAACTACACTAATTGAATCATAAGACTTGTTAGGATTAATTAATTTAACAAAAATACGTAAAATTAAATCTTGAAGATTATCATTTTTTAAAGTTCCTATTAATAATTTATCAAACGCATTTTTTAATTCATTTTGATTATATTTTATTGTTAATGATAAAATTGTTGCTAAATCTGTTGCTTCAGCATATTCATCAGCGTGATCAATTAAGTTAGTTATTAAAACATTAATTAAATTTGATAATAATTTTTCTTTATTTAATAATTCAACAATAGCCTTAGCAAAGTTTAAACGTTGTTGATTTGATAAATTTAAATTTTTAAATAAATCAATTGAACTAAATAATTGTGATAATTGATTATTAAAAAAATTCATAAGATTAGAATTTTCAAAAATGTTTGTAACACTAGTTGTTAGAATAGACTTAATTTTAGCTTTATTAGATTCAATATTAATTTTATTATTTTTATTATCATTTACTTTTAAAATTGGTGAATTCAAAAGATTTTTTATTAAATCGAAAATTAATTTTTTAGGTTCTAAATTTAATTTAAATTTAAAATAAAATTGATTTAAAGTAGTACTATCTGTTGTTTGGTTATTATTAGACTTTATATTGACAAATAATTTATTAAAATCTAAATTGTCTATAAATATTTTAATAACATCAATAAAATCTTGATCTTTAATTTTATCGTTATCAATTAATTCAATCAGGGTTGATAATTGTGGCAATATGTTTGATAATAATTTGTTAATCCCATCATTTAATATTAATAAAGTGTTTTTAGGATCCACTAAAATTTTATTAATATTGTTTGTTAAAAATTCAAAAGCTTGTTTTCGGATTTCTTTATATAAAGTTAAATTAGGTATTGTTGGCAAAATATTAGTGATAAAATTATTAATCTTTTGTTTTTGATCTACTGTTAATTCTTTAACAAAGAAGTTTTTGACAATTAAATTTGTTGTTAATGTTCCTACTAAATTTGGTATTTTTTCATTGTTAATAAGTTTTTTAAATAATATATCAACCTTATCTTGAAGTTCTTTATTTGTAGATTTTAAAATTAATGGTAATAAATCATTAATATTATTTAAATTATCATAATTTTGTGGATGATCAATAAAGTCATTAATAATTGAAAATATTAGATCATATGATTCAGAACTATGAATTAAGTCATGAACAACTTCTTTGATTAATTTTCAAATATCAGACATATTTGATTTTAGTAAACTATTATTTGCACTAATTACCAGATCGCCTAATTTATTAGCAATAAAATCTTCTATAGAAGTAACAAAATTTTGAGTTTGTGGATTTTTTAATAATTCATCAACACTCATTCTTAAAAGGCTTTTTAATTTTGTTTTTTGGTTGGTTGTTCAATCTTTTTCCTCTAAAACAGCAATTAAATTTTTTAATAAAACATTAAAATCAAAATGACTGGTTTTTAATGGTGTGGTATTAGAAGAAACTAAATTAACAATACCATAATTAAATATATTTTTAATTGTTGAAGAGTTTAAAATTGTTTTAATAAATTCTTTTCATTCGTTTAAATCAACATTATCTGATGTTAATAAATCTAAAACATCGCTAATAAATGATTTTGAAGTAAAATCAATGGATTTTTGAATAAGATTGATAATTGAAGTTTCTGATGAATCAAATATGCCAGCATATGTTTTTGAATCAGTTAATAAATCAATCGTATTTAATTTTAAAGTGTTTATTAATTTTAAATTTGTACCTTTTGATAAAATTTTTTTAATTATTAAAGATGCTTGCGGAGTTTTTATAAAATCAGGAATATTTTGGCCTAAAACTCTTATTAGAATGTTATAAGTTGCATCTAATGTTGTTTGATCTTTAAATAAATCATTAAATAAATTGTCAATTCTAGTTGTTAACAATGCTTTATTAGTCTTAACAATTTTTGCTAAAACATTTGATAAAGGACCCTGCAATAAATATTCATTATCATTATCAAACAAAGCATCTACAAAAACATTAATAATGTTATCTAGTGATTTTGTTTGAGTTAATTTATTTTGGATTATTAGTAAATTTTTTAAGAATTCATTATTATTCTTTAAATTATCAGTTGCAAATTCACCAAAAATTATTTCTAATAATTTATGGGAACTAACCAAATCACTTAATAAAGCTTTTATTAAAGATTTAATTTGAGATTTATTTTCATTGAATAGCTGAGAATGAAACATTCCTTTAATTAATTCATATAAATTCTGATCATTGATAATATTTTTTCTAAAAACATCATATAAAATTTTTTTAGAAATATTTTGTGTCCCAATTATATTATTTTTATCTAAATCTAAATTATCTAAATCATTTTGAATTCCAGAAATAACCATATCAATATATGGTGTTGATGCTAAAGCTGATGCTAATTTATATAAATTATTAGTGTTATCAGCATCTTTTTTTGATAAAAATTGACTGATATATCCATTTTTATCTAATCCTAATGATGTTAAATTTGAAGTAATTAAATTAACAAAACTCCCAAAACGTCGTTTATTTGAAATTAATCAATCTTTAACAATTGTTGAGGTATTTTTAAAATTAGAAAAGGATTTACGTAATTCTTCATCATTTTCAAGAGCAGTTTTCGCTCAAAATGCCGCACCTTTTGAAGAACCTAAAGTTTTTTCTACTAGATCTTGATTTGTGTTATTAGCAAAACCAATTTGTTGTTTAAAACTATCGCTATCTTTATTAAAATATTCACTATCTCATGCATCATAAATAGCATTTGCAATAGAAGCAGTTTTTTGCTGAGCGTAATTTTGTCCCAAAGACATTTTTAGAAAAATATCTTGAGCCATCCGTTTATATCCTAATTCAGTTGGATGAATATCAAAAATTGCTTTTGCAAAATTAAAACTGTTTTCTTTTCAATCATTCTCATCAAAAGCATCGATATAGTTGATATTATTATTTAAATCATATGTTTTTTTAATGGCACTATTTAGAATATTTATAATTTCATCACTTAAACGACGTTTATTTGTTAATTTAAATGAACTATCTATTACATCTTGTAAACGTAATAGTGGTAATGGATAACTTACTAATGTAATAGATACATTAGAATTTAAAGTACGAATAAGAGCGATTAATTTCTTTAGATTTTTTGTAATTTCTAAAGATGCCTTTTGTAAATTATTTTCGAACTCTTTAACTTTTATTTCTAAATCATTACCTTTCAAATTTGTTAAACTAACAAGTTTTGGAATGTCTAAATATCTTAAAAAATCATTAGCACCTAAGGTGATTGTCATTAAATTAGCTTTTTTAATTTGCTCTTTTAAACCATTAAAATCATTAATATTAACTTCATGATTATTTTTTGGTGTTAATTTTGAATCATAACCAAAATTTTTAAAAAACTTTGATAAACGGTTTTTAAAAGGATTATCTTTTTGTTGATCCATTTGTTTAGAAAAATCAAAAAATCTATATTCTTGATTTTTAAAATACTCATCACTTCCAGCACCTAACATATATAATCAGTCTTCGATGCGTGATCCTGTGATTCCTAAATTTTCATAAGAAGCTAAACGATTTGGTTCAACTTTATTTATGTATTGCGCAATAAATGAAGGAAAACTTAATCCACTAATTTTATTAGTTATTGGATCATAGCGACCTGGGGCTTCTCAACCTAATTCTGAATTAAAACCAGCTGTGATTGAATCACCAATCGCTAAATAATTAATTTTTGTTTGGGGTTTAATACTTACCTTACTAAAATCAAGTTTAAGATCTGTATTTTGATCATTAATATTATTTAAATCTTCAACTTTGATATCATCTTTAGTTTCACCAGGTATTTTTCGCGTAACATTATCTAAATTAATATCTTTTTTACCCTCTTCTTGTTTTTGTAAACCAAAATAAGTAGATGGAGCAATAATCGCAAGTGCTGCAATAATTGAAGCAACACTTAATCCTAGAATTTTCTTACTTTTTTTATCCATATTGAACACTTCCTAACGTAATTTGTTTTAAAATGGACATTTATAAAAACAATAATATAGTAAAAATAAGATAGACAAAGATCTAGTAAATAAATTACAAAAAATCTTTAAACATCTTTTTATTTTTAATAATTACCTAATATATTTTAGCAAAAATAATATCATAACTTTTTTTAATATTTTTTATTAAATATCAAAAGCAACTAATGTTTTAATAGATACTTAGATTCCATCTTATACTTTATAATAAATATTACAAAAACTAACTAGTATATAATATTGTTAAATTTAGAGTATAATTTCAATGTTTTACATTAAAATATGGAAAGGCTAAATATGAAATTAAGTGATTATCCGGTCTTAGATATCGATAAAAAATTGTTTAATTGATCAAAAGTTAAGAATAAACTAGTCTTAATTGTTAATGTAGCAAGTAAATGTGGATACGCTAAACAATATGAACAATTAGAATATTTATACAAAAAATATAAGAATAAAGGATTCATCATTGTCGCTTTTCCTTGCCGTCAATTTATGTTTCAAGAATTTGATGACAATAATAAAATAAAGGAATTTTGTTCAACAAAATACAATGTTACTTTTCCAATAATGGATCTAACTAATGTTGTAGGTTCTAATATTTCTCCATTATACAAACAATTAATTACTGAGTATCCATGAAGTCCAAAAGCAAAAGCAGTAAAATGAAATTTTGAGAAATTTTTTGTTAAAAATGATGAAATTATTGGACGTTTTGAATCAAAATGTGAACCAAATGATTTAGAAGAATTTATTAAAAATAATTTATAAAAATAAATGGCAGTAGAAAAAATTAAAATTTCAAAAGTTAAATTAAAAATTGTTTCAGCTTTATTTAATTTGTTGAAAACCAATTCATTTAACAAAATTAAAATTTCACAAATTATTGAGGTTGCAGATGTTTCGCGACCTTCATATTATCGTAATTTTAGTGATAAAATTTCGATTATTAATTATTTTATTAAAAATATTTATAATTGGAAAGTTGTAAGTAAATTAATAAATAATAACCTAAATATCAATTTTGATGAAGTTGGTTTTACTAAATTTTATGAAATTTCATTACAAATTAGTTTAGAATTCAAAGAATATTTTCTAATCTTAGATAAAAATAATTTTAGTGGATTAATTGTTGATTTAACCACTGAAAGTGGTTTTTATGCTATAGGTAATATGTCAAATAATTCAATTGATAAATATTTAATTAATTATTTAACAGGATTTTTTTGTAATATTAGAATGCAATGGTTAAAAAATGGTGCTAAAGAATCAATTCATGATATGGCGGTATTTTTAACTAACGTTTTAAAAGATAATGTTATTAAAAAATTAGAGAAATATCAATCTTGTAATAATATTAATGATTAAAAAATATAAAAACCAGACGCAAAAACGCCTGGTTTTTATCTGAATTCTTTTTTAGATAATGATAAATCAACATGACAATAACTATTAGGATTTTTATCTAAATATTTTTGATGGTATTCTTCAGCAATATTAAAATTGCGTAATCTTTCAACTTCAATCATAATTTTTTTTGGTGTTTGATTTTGTTTTTTTAATAAAAAATCTTTAATAATAAACTCATCATCGTGATCCACATAATAAATACCACTACGGTATTGACTACCAACATCATCGCCTTGCTTATTTAAACTAAAAGGATCAATGATTTGATAAAGATGTTCTAAAATACTTATTAAACTAACAAAACGATCATCATAATCGATTTGAACAGTTTCTGTATGGTTGGTTGTTTGAGTGCAAACTTGTTCATAACTTGGATTAATAACGTTTCCTTGACTATAACCAACAACTGTATGATTAACACCAATAATGCTATCAAAATATTTTTGAATACCTCAAAAACAACCTCCAGCAATTCAAATACTCTTAATCATAAATTTATCTCTCTCTATTGACTTTCTTATTTTACTATAATTATTCTTCAAAAGGAATAATTTTTTTAATTCATTGATCACGTTTATCTAAACCATAATCAATAATTATTTCATTAATATGATTAAAGAATTTTTGCGTTGCTAAAAGAACATTTTTAGGGTCATTAATATCTAAATTATGATATGCAACGGTCATAACATAGTTAATTAAAAATTCTTTTCAATTCTTGAAATATTTAATTGTTTCTAATCCAAAAACTGGTATAAGATTACTAAAATGCTCATTTTCTAAAAAATGTAAATCTAGCCCTGTTCGCAACAATAAAATTACTTCAGAAATATCATAACTATAGGCAAAATTAATAGAGATTTTTGCTCTTAAAAAATTAATGAAAGCAAATTCTAAATCAATATTTTCTAAACCTTCATTCGATATAGTTTTTAAAATATTTTCATGTTGTTTTAAATCATAAATTAAACTTAAACGTGCAATATTTTTTGCATTTTCTAAAGGAATTTGATTATCCATAAACGTAAAAAATTCTTCAATATTATATAAATTAAAATAATTATGCAAAACTTTAATCTTAAAACGATGCGCTACTTCATCATCATTTGTTAAATAAAGCGAAGATGATTTATGATTTAAACCTAAAGTTTTAGACAAAAGACCAGATAAACATAAAGTAAAATCAAAAATTTGTTTGTCGTTTTGATGATAAATATTTTTGTTATTTTTATCAACAATAATATTATTAGTCAATGAAATTAATTTATCATATAAATCATTTCAAACTAACAAATGCTCATGATAATTAGAAATTTTTTGTTTAAAATATGTTATATTTGGGTTAATCTTTATCTTTTTATTAATCATTTTTTATTTTTAAACCTAATTCTTTTAAATCTTCATCATTCATGTTAGATGGTGCGTCTAACATCATATCAACGCCGTTGTTATTCTTAGGAAATGCAACAACATCGCGAATATATTCACTATTCACTAAAATCATCATCAAACGATCTAAACCCAAAGCAATTCCACAATGTGGCGGAACACCATATTCGAATGCAGTTAGTAAAAAACCGAATTTTAAATTAGCCTCTTCATCACTCATATTAATTGATTTAAACATACGGCGTTGAATTTGTTGGTCTATAATCCGAACTGATCCTCCACCTAATTCATAACCATTTAAAACAATATCATAACTACGACCTCTAGCGTCTTTTTTATTTACATCAAAAGTATTTAATGTTTCTAAAGTTGGCATTGTGAAAGGATGGTGTGCAGCAACAAAACGTTGAGCCTCATTATCATATTCATATAATGGTCAATCAATAATTCAAACAAATTTAAAAATATTGGGTTTTTTTAAATTAAGAATAGATGCTAAATTTATACGTACTAAACCTAATGCTTGAGATGCAATATCAAAAGTATCAGCAACTAATAAAATTGATCCAGTTGATAAATTAAAGTCTTTAAAAATCGTTTTATAAATTATATGATCTTCTTGAATTGATAATAACGAACCATCTATAATTTTTTCATCTTTAATACTTATTCAACTTAAACCTTTAGCACCTTTGTCTTTGGCAAACTTTTCAAGAGTTTGTACTTGTTTTTTATTTAAATTAATATTAGGTACTAAAATTGCACGAATACTTTGATTATTTAAAAGTGCTTTTTGAAAAAATAATGTTTTTGAATTTTCAAAATATGTGTTAAGTGTTTTAATTTTCAAATCAAAACGTAAATCTGGTTTATCACTACCATAATCATTTATCGCTTGCTCATAACTCATTCGCATAAAAGGAGTTGTTAAATCAACATTAATGGTTTGTTTAAAAACATATTTTAATAAATTTTCAATTAATGTCTGAATTTCTATTTCATCAGCAAATGATAATTCTATATCAACTTGAGTAAATTCTGGTTGACGATCGCTTCTTAAATCTTCATCACGAAAACATTTTGTAATTTGAAAATATTTTTGAAAACCCGCAACCATTAATAATTGTTTAAAAGTTTGAGGTGATTGTGGTAAAGCATAAAATTTTCTAGTTCTTGTTGGCACATAAAAATCTCGTGCACCTTCTGGCGTTGGTTTTGCTAAAATAGGAGTTTCAGTTTCAATAAAGTCTTGTGAATATAAGTAATTTCTAAAAGCTTGGTAAACTTTACTTCGCAATTCAAAAATTTTTAAATTAACATCACGACGTAAATCTAAATAACGATATCTAAAACGAATTTCCTCATTTGCATCAGTTTCATCTTCAATAATTAATGGTGGTGTTTTTGCTTTTGAATAAATAAGTATTTTTTTGACATGAATTTCAAATCTACCTGTTTTTAAATCATTATTAGGGTTTTTTCTCAACAAAACTAAACCTTCAACATTAATTACATCCTCACGTGAAAGTTGTGTTAATTCATTAAAAACACCATCACTTTTTGTTGCAAAAATTTGTACAATTCCAAATCGATCTTTTAGGTCAACAAAAACTAAATTACCCATTTTACGAACTTTTTTTACTCAACCATTTAAAATAACATTTTTTTCTAAATGTTCTTCACCAATATAACCACAATAAACTTTCATTTTTATTACCTTATTCCTTTAATTTCATCTAAACTTAATAAATCTTGTTTAAAATTAAGTAAATCTTTTAAAATTATTTTTTTATCTGATCATTCTTTTTTAGCTAATATTATAAGCTTTTTTACATTTAAACGTTGTGCTAACTTAAAAATTTTGTCAGCTTTAGTTGAACTTAAGATAATGTCAACATTTAATTGATTATTGATACGTAATATTTGAGTTAGTTTTATAGCTTCATTCTCTAATTCTTTATCAAAAAATGCAATCAAATATTTATCATCATCAGTGTTTAAAATATTTTTATTGGTTTCAGATAAAGCTATTAATAAACGTTCAATTCCTATACCAAAACCAATACCTTCATAATTAGGGCCACCTGTTTGTTTAATTAATTCACCATATCGGCCTCCACCTATAATTGTTGATTGACCTAATAATTTAGGTGAAGTTGAAATAAATTCAAATACTAATCCACTATAATAATCCAAACCTCTTACTAATCCTTCGTTAACATAATATTTAATGTTATAATCATCAAGCATTTTTTTAATTAGATTAAATTCTTCTTTTTCTTCATTTGATAAAAAATGACTAATTTTTGGAGCTTGTTTTACAACTAGTTTTTGAGATTCTAATTTATCATCTAAAATACGCAAAGGATTGGTTGTAATACGTTCTTGACTTAATGGAGTTAATTCAGTACGATGTAAATGAAAATATTCTTGCAAACTTTTAACTCATAATGAACGATTATGAGGAGATGAGATATAATTAATTTCTAAAATATAATTAGATATCATTAATTTTTTTAAAATTGTATCAGCTAAAGCAATTAATTCAAAATCATAATAAATATTTTTTGTTCCCAACACTTCAACACCAAATTGATGAAATTGGCGTTGACGTCCAGCTTGAGGGCGTTCATAACGAAAACAAGGCTCAAAATACATTAATTTTAATGGTAGTGGATGATTTAATAGTAATTTATTCTCAACAACAGCACGAATAACACCTGCTGTTCCTTCAGGTCTTAACGCTAGTCAGCGATCTGATTTATCTTTAAATAAATACATTTCTTTTGAAACTAAATCTGAACTTTCACCAGCTGATTTCATAAAGAGTTCTGCAAATTCAAAAACAGGTGTTTTAATTTGCTGAAAGCCATATCTTTTAGTAGTTGATAATAAAAAATTTTCAAGTAATTTAAAATCATTCATTTCTTTATTATATAAATCTATAGTACCTCGTGGTTTACTGTAATTAATCATATTCTAACTTTCTTTTATTTTTAAACTCATCTTTAATAAATTAATAAAATAAGGATTTGCCTTTGAAGGTTTTGATGTATATTCTGGATTAAAAATTGTTAAAACATAAAAATGATTTTTTGTGTATTCTAAAACATCAATATAATTATTATCCACTGAAATTGCTCCAATTTTTCAATTTTGATTTAAAAAATAATTATTAATATAATGATTGTTAAATTCAAAATGATGACGATGGCGCTCGTTCAATTGATTTTGTTTGAGATAAATTTGGCTTGTTATAGTGTTAGCTTGAATATTAGAACAATACTCTCCAATTCGATAATTTTCAATTTCATTTTTAACGAAAGCGTCTTTTAAAAAACTAAAATCACCATTGCCTAAATTAATAAATTCAATTGGAAGTTTTAGTACATTATTAATGAATTCACCAATACCAATATTAACGCCAGTTCCCACAGCTAAAAATGGAATATTGTTTTTTCTTGCATAAGATGCAGCTAATAAAGCTCCTGGTCAGCCTTTTATTGAACCTGCAATACTTGGAATAACAATACCATTTATTTTTTTTAGTTCTTCATTTATGTTGTTTTCATTTAAATTTTGGGGTTGAATATATTTAATCTTTAAATCAATATTTAATTCAATTGCTGCTAATTCTAAACTCGCTAATACTGATTTATAAGCATCGGGTAATTCAACATATTTTCCAACTAAACCTATAACTAAATTATGTTTTTTAAAGGTTGTAATTTTATCAGTAAAATCTATTCATGAATTAATATTATCATTTTTTAAAGGTAGACTAAAAAAATCTAAAATAGTTTTATGAATCCCTTGCTCAAACAAAACATTTGGTAATAAATAAACTGACTCAACATCATAAGCAGCAAAAATCATATCAATATCTAATCCACAAGATCATGCTAATTTTTTAATCGTTACTTCATCTAATTTTTGATTAGTTCTTAATAACAATAAATCTAAATTAATTCCTAATGAACGTAACGTCTTAACGCTATGTTGAGTTGGTTTTGTTTTTAATTCACCTACTTTTTCAATATAAATAAGCGGTGAACAGTGAATAAACATTACATTATTTACACCATAAATTGTTTTAAATTGACTAATAGCTTCAATAAATGGAAGCGATTCAATATCTCCAATTGTTCCTCCAATTTCAACAATAATAAAATCAGTTTTTAAACTATCTTTAATTTTTTTAATGTAATCAATAACTGCAGATGTGACATGTGGAATAGTTTGAACAGTTTTACCGTCAAAACCATTTTCACGCTCTTTTGTTAAAATTTCATAATAAATTTTTCCTGAAGTTACTGACGCATATTTATTTAAATCAGCATTAATAAAACGTTCATAAGTACCTAAATCTAAATCTGCTTCTTTACCGTCTTTTGTAACAAATACTTCTCCATGTTGTAATGGTGATAAATAAGTGGGATCAATATTTAAATATGGATCAAGCTTTTGCATTGCAACACTATATCCTAATTCAACAAGAATTCGACCAATACTACTAGCACTAACCCCTTTACCTAATGATGAATAAACACCACCAGTTACAAAAATAAATTTTGTTTTTTGTATATGTTTCATTAGATCAATCTCCATTCATTAATAACATTTTTAGCTTGAAGTTCTAAAATAATCTTTTGTAAGTGATCTAAGTTTTTTACATAAATTTTTAAAAAAGTATCATGAGTGCGTGCAGTCTTATTTTTACTAATATCAACTTTTGAAATATTTGCTTTCATGCGTATTAATGTGTTGCATAAAACATTTCCAACAGATTCACTTCAATTGCCTTTTAATGTTAAGTTAACTTTAAAATAACGCGGACGATTCTCAAGTTTTTCTTTTGATCATTCAATTACAATTGTTTTTGAGCGAGAATTTTTAACTTTAGAACAATCATTGCGGTGAATTTCTACTTTATTGCGAACAATTTTAGCAACACAATTAATTCCAGGAATTTTAGAACAACAAGGCGAAATTTCAAAATCTGTAAAATACAAGTTCTCTAGACCACTAAAATATGAAGAATCAGCTAATCATTTTCATGCTTGATTTTTAATTGTATTAATTGCTTCACGTGAAACTTGTTTATTTTCTGAAAATACTTTATATTTTGTTTGTAAATCAATGTCAGTATTAGCAATATTATCTAAAAAAGTATTGATACTATTAACTTGAAATTCTTTTTCTAGTCTTTTAATAGCATGCTCTTCACTCATAATTTTGCCTTCTAAAAAAAATTCAGCATTAGCTAAAAATTGTTCTTTTGTAATTTGTTTATTCAGTTCATATTTTTTTAAATGATTGTTAATATGTAATTTAACAACATCATTATTAGCTAATGTAATCCAGTTTGGTCTAATTGTTTCAATTCCATTTGAATAACTAATTTTAATTGTATCGCCAGTTTCTACATGCGTGTCAAAATGTGAACGCTCACCATTCCGATAAATTGCTAAAATATTGAAAAAATTATTTATATCGTATTTATATGCTACATCTAATAATGTTGAACCATTTGAAACAGTGATTCATTTATTAGAATTAAGAATTAGTACATCAAAAATAGTTCCGGTTGTGACATCTTTAATAGTTTTAATTCCTTTATCTGTGCTCAATAAAAAATCACGCAATAAAACATTATTAACTGTTAATAAAATTTCAGTTTTATTATCTTTTTGTTCTTTATATTTTCAGTGTGCTGCTAAACCATTATTAGCAAATAAATTCATTTTTGTTGTTCTGATTTGAATTTCTACTAAAGTTCCTTCATACGAAATTGTTGTATGAATAGATTGGTATAAATTTCATTTTGGTGTTGAAATATAATCTTTAAAAGTGTTAATAATATACGTAAAATTCATGTGTACTAAACCTAATGTTTGATAACATAAATATACATCATCTAAAATTACACGTATAGCATAGATATCATGAATATCTTTAACTTCATAACCACTAGTTATTTTTTTATATGTTGAGTAAATACCTTTAAGACGCCATTCAAATTCAGCCTTAATATTATTAGCTATTAAAATATTTTTTAAACGAGCTAAAATTTCATTTCATTTTTTTTGATTGTTTTCAACCAATTTATTAATTGAATCGTTTACTAATTGATAATTTTGTGGGTCTAAAATTTTAAAAGACATATCTAATAAATCTGTTTTAACACGATACATCCCTAATCTTCCAGAAATATTTGCGTAAATTTCTAGTGTTTCTGTTGCAATTCTTTTCGCTTTTTCTAAACGTAAGTATTGAATAGTTTGCATATTATGATAACGATCAGCAATTTTAATAATCATAGCACGAATGTCTTGTGTCATTGACATAAACACTTGAATTGAATACTTTTCTTCAAGATTGGTGTGTGAAACTTTATTACGTCGATTTTTTGAATAAAGAGATACTTTTGTTACAAAACAAACAAGTTGCGTCACTTCTTTACCAAAAGTTTTTTCCATAACTTCTTCTGGACAATTTGTGTCTTCTAAAACATCGTGTAACATACCTGCTACTAATGTACTTTCATCCATATCTCATGAAGCTAAAATTCGTACAGTTGCAATTGGATGAATGATATATGGTTCTCCCGACTTTCTTGTTTGATCACCATGATAATAGTTTGCAAAATCAAATGATTTATGGACTAATTCGATGGTTTGGCTTGAATATTTTTTTTGTTTAAGAAAATCATATAAATCATCTAATTTTTTTTTAACTAATTCATTCATAATTACTCCATTATTAAAAATAAGTTATAATCATTTATTATACTTATTTAAATAATAACAAAACACGAGTTATTAAAACTCGTGTTTTCATATTATTAATTAATTATTTTCTGATACAATGTACTCTTCTACAAATTCATCGTCATCATCAAAATCATTATCATCAATCGATACTCTTTCATTATCAATTGAAATACCAGCATTAATAATTTCTTTAATTCCTTTAGTAAAAGTAAATTTAGGAATTGTTTTCTCGGGAATAATTACAGTTTCACCCGTTTTAGGGTTAATTCCCTTACGTTCTGCACGTACACTAACCTTGAATTTTCCAATATTTCCTAAACGAACTTCTTTAGCTCGTGATAATTCAAGAATTAAAAAAGCTTCGTAAGTATCCATAAATGCTTTTGTTTGTTTTTTTTCAATATTTAACATTTTTGATAATTCATCTATCATTTGTGAACGTGTTTTGGCTTTAATTTTTGCATTCATAAGGCTTTGTTCTTTCTTTGAATTTAGTAAATGTTATTTTTATAATTAATTTTATAAAAATTAACCATTAAATAATAATTTATTTATTTTTAAAATTAAAGTAATTTAATTGCGCTTTCTAAGGCTAATGTTACCATTTTTTTAAAAGTATTAGCACGCTCTGCGGGTGATAGTGCATCACCTGTAATCAATGAATCACTAACTGTTAGTAAAGTTGCTGCATTTTTATTTAAAATTTGTGCATTTGCGAATAAACCAAATGATTCCATATCAACACAATCGGATTTAGTTCGATGAATAATTTCATCTAATGTTTGAAAATCATTATTATAAAACACATCAGAAGCATGACAAGTTGCTAATGATAATTCTAAATTTAATTCCTTCGCAGTATTTTTAATTAAATTATTAACTTCTCTTGATGGTAATAAAACCTTATCATCGCCAACTTGTGCACCAATCAGTGATGCGAATGTAGAATCACTATATGATTTTTCAACTAAAACCACATCATTAACATTCAAATCTTTTTTATATGAACCAGTTGAACCAACACGAATAATTGTTTCAACTTCATAAAATTTATATAATTCATATGAATAAATTCCGATCGAAGGAATTCCCATACCATGGCCCATAATTGATATTTTTTTGCCTTTATATGTTCCTGTATAGCATAGCATGCCACGAACTTCATTAACTAAAATTGCATTTTCTAAAAAAGTTTCAGCAATTCATTTAGCGCGCATTGGGTCCCCTGGCATTAGTACTACTTTAGCTATTTCTCCTAATTTAGCTTGATTATGTGCAGTCATATTTTATTCTCCTAAAAATAAATTTATTACGATATTTAATTTTATATTAAAAGGTTTTTATTTGATATAAAAATTATTTAAATTTTACTTTTTCAAATTCGTTTTCATCAAATACTTCTAAAAACTCACGTACTAAGTCAAAGGCTGCTTTTATATCTTTAACCGTTGCTACGCCAATTGGAGCATGTAAATAACGTTGTGGGATAGACAAACCAATTGTTAATACTCCACCATTAGGCCCATATTGCAATTCTTCAGCATCAGTCCCGCCTCCTCGTGCAACATATTTATATAAAGGAATGTTGTGTTTAATAGCTAATTCTTCAAAATATTTTACTAATTTAGGATTAACTAAAGTTCCACCATCTTTAATTCTAATACATACACCATCATTTAATTTTGTATCACCCTCTGGACACTTGTATGTATCGTGTGATGTTGTTGTATCAATAACAATCCCAATATCAGGTTTTACTTTTGAAACAACTGTTTTTGCGCCACGTAATCCAACTTCTTCTTGCACTGTTGCAGCAAAATAAGTTTGGTTGGGAATTACTTTGTCCTTTAAACTATGTATTAATAAATCTAAAACCGCAACACTTACACGATTATCAACAGCTTTACCAACAATATAATTTTCATCATAAAAATTTAAAGTTGGTCCTTCCATATAAACTTCCGTACCTATTTCAATTCCTAAATCAATAGCTTGTTGTTTTGAATTAAAACCGCAATCAACAAATAATTCTTTAACTGGAACTGCTTTACTGCGTGCTTCAGGTTCTAAGATATGAATTGAAGTATGGCCAAATACACCAGTATATTGACGTTGTTCATCAACATACACTTTTGCTACTGATCCAATAGCAATATTAGGCCATACACCACCTACCATTGATAATAGTAATTGACCATTATCAAGAATTTGTTGAACTACAAAACCAACTTCATCCATGTGTGTAGCAATTAAGATTTTAGGTCCTTTATTTGAATTTGTTTTTTTAAAAATAATTGAACCTAAATTATCACGTTCATAATCAACTCCCGCATCTTTTAATGATGCTTTTAGTTTGGAAGCAACACGTTCTTCATGACGTGACATACCATATATTTCCATATATTCAATTGCTTTATTTCTAATTTCTTTTTCGTTAAATTTACTCATAAATTACTCCTTCTTTAAAACATTTAAAGGAACATTATCAATGGTTTCAAGACCATCAAGAACTCCTTCTTCAATATATGATAAGCTAGCGCCCCCACCAGTAGATATAAAATTAATATATTTATCTAATCCTAGGTATTCAGCATGACTCGAAGTATCGATATCACATATAATTGTATAAGCGCCACGTGTTGTTTGTTTTGCCATTGATTTACATATTTCAGTAGTACCTTGTGCATAATTCTTAATATTTTCAATAACACCAAAGGGACCATTTAGTAAAATAGTTTTAGAACGATAAATTTCATGTTTAATAAATTTTAAACTTCTTTTACCAATATCTAAACCATATAACCCTTCAAGACCTTCATCTATTTTACGATAAATCGGTCTTGTATCACTAAAATCATTGTTACATAAAAAATCAAAACAAATTAAAATTTTTTTACCGTATTTTTCTAATATATTATTGCAATCATCAATCATATTGCGTTCTACTAAATTTAAACCAACGTTATACCCTTGAGCATATAAAAATGTATAGACTAATCCTCCCCCAATAATAACTTGATCAGCCCGTTCACATAATACTTCAATTGCTGCAATTTTATCAGCAACACGGTTACCACCTATTAAAGCTAAATACGGACTTTTAGGAACTTCTAAAGCAATATCTAGATGGTTAATTTCATTAACAATTAAAAAACCTAATGCACTTTTTTTAGCAAATTTAGCTGTTTGATAATTTGATGATAATTGTCGATGAGCAACTCCATATGCATCATCAATAAAAATATCCCCTAGCATTCCCCAAAACTGTCCTAAAATCTCTGAACCATCTCATTCAAGCCCAACATATTCACCTTCATCACTAATATCACAATAACGCGTATTTTCTAAAATTAAAATGTCTCTTTCTTCTAAATCATTAACCATTTGTACGACCGTTTCTCCATAATTTAAAGGTGAAAATACAACACGATTAACATTCTTGGATACACGATTACGGAGTTCATCAACAATAATTTCTAAACTATATTTACCACTTTGTTTATCTGCTAAGGTTTTAATTCGACCTAAATGACTTAAAATAACAATTTTAGCGTTATGATTAATTAAATAATTAATAGTTGGTAGTGATGCTCGTAATTTACGATCATTTAATATGCGTTTATTTTCATAATCAACAACAACATTTAAATCTAAATGTAAAACTATTGTTTTACCATTTACATCAAGATCTTTTATACTTTTTTTATTTAAACTATTCATTTTTAATACATCTGATCATATGGAGTTATTTTCATAATTGCCAAACCATTAGCTAAAACCTGTTTAACACATCATACTAATGTATAACGTTGTGCTGATAGAACTTTATTATTATCATTATTAATTTTAACATTTGCATAATAATTATGAAAAATTTGAGCTAAGTCATATAAATAATTACTAATTCGGTGTGGTTCACGATTATTTGCAGCATTAGCAATTGTTTGTTTATAGAAATGAAGTTGATTCAACAATTCACGTTCACGTGTTTCTGTTAATAGATCAGTTTTAAAATCTAATTCATAAACTTGTTTATTTAATACTTGATTGGCACGAGCATGTGCGTATTGAACATAATATAAAGGATTATTGTTATTTTTTGATAAGGCAATATCTACATCAATAATAACATGCGAATTCATAGATGATGACCCTAAAAATCAACGTAATGCATCTTTACCAATAATTTCAACTAAATCCTTGATTGTTAATGATTGTCCGCTCCTTTTTGAAAGTTTAAATTCTTGATTATTTTTAACTAATTTCATTACTTGAGCACAAACAATTTCTAAATTATTTGGATCATAACCTAAAGCATTCATTGCCGCTTTTAAACGCGCAATATAACCTAAATGATCTGTACCCCACACATCAATTAATTTAGTTGTATTTGTCTTATTAAATTTATAGTCATGATAAGCAATATCTGGTGTGAAATATGTATATGAACCATCAGATTTAATTAAAACACGATCTTTATCATCACCAAAAGTAGTTGTTTTTAATCACAAAGCACCATCTAATGTATAAGTGTAAGGTTTTATTTTTGATAAAACTTCTAAAATACGTCCCGAATTTCGAATTCAATTTTCTGAAGTATAAGTATCAATGAATGTGTTAATTGATGCTAAATCGTTTTTAATTTCATTTAATAAGTATTTAACTGCAAAATTTTTAATTTCTTGATTAGCAATATCATCATCAATTTCATATTTTTCATTTAAACGTACATTAATAAATTGATTTTTATAAGTTTGGTATAAAGTTTGTGCAACTAAATGAATTTCTTGCCCATGGTAGGCATCTGCTGGTAATTGAATATTAATATTTTGTAATTGTAAATAACGAACTAACACTGACATTGCTAATTTATCAATTTGATTTCCAGCATCATTAATTCAATATTCAGTTTCTACATCATATCCATAAATTTTTAATAAATTAGCTAAAATATCACCATAAATAGCATTAGCAGCGTGAGCAATATGCAAATAACCTGTGGGGTTAGCTGAAACATATTCAATATTATAAGTGATATTCTTTTTAGAGAATTGACCAAATCTATCTTTTTCATAATAAATTTGTTTCAATAAATTTTCATGATCTTTTGCTTTTAATTTAAAATTTAAAAATCCAGGTGGCTGCAAATTAACTTCTAAAAACAAATCTTGTTCAAAATTATTAGCTATTTCTTTAGCTATTTCTAAAGAACTCTTATTATTTTTTTTACTTAAAATCATTGCTATGTTAGTATAAAAATCACCAAATTTAATATTTTTAGTTTTATCAACGAGAATCTTTGTCTCCTGTGTATCATGAATACCCATTTTCGCCAAAGCCTTATTTAATTCTTCACTTATTTTTTGTGTAATCATATTTAATTTATTCCTTAAACATAAACCATTTTTATAATTCTAGCACTTCTGAAAAATCTTAATATTAAAGAAAAAACCAACCAAAACATTCAAGTAATGGTTGGATTCATGTGCGTTTCTAATTTAGAAACTACGATATTTATTAGCCTCTTTAATTTTTTCTTTACGCCGAATTCTTGGACTTAAATAATATTCGTGACGTTTAGAATCTTTTTTTGTTTCATTAGAAATACGTTTAAATTTCTTAAGAGCCTTTTCTAAATCGCCTTCAACGCTTACACCTCTTGACATAAATTCACCCACATTTCTGTTAAAAATAGATTATTTAAAATTATAATCCTTTTCCAGCATTAAATTCTAAAAACTTTAATATAAAATCATTTCAGAAAAATTCTTAGCGATTTTACGGCCAACTTTAGCACCATAAACATTATCTAAACAAACCAAGTCAGCAGTGATGTGAACTGATAAGTGACTTAAATATGTGCCTATTCCATAAAAATCAATTGCAGAATTGTGTTTATTAAAATTTTTAATACCATTTAAATCAATCCCACTTGAAGCAATTATTTTAATATGTTTTCCTCCATTATTATCTAAATATTCACGTACTAAAGCAATAAGATAAGGATTACAACCATGTAAATCACGATTACCAACATTATTAAAAGTCGATAATAAACTTTCATCAATTAAATCCTTAGCTGTATCAATTCGTACTGCAAACAAACGATCAAACATATATCGTAGTTGTTCAAGATCATTTAAAACATTGTTATTATAATCAATAACAGCAATTAAAGGATCGCTAGGAAAAGTTTGTGCAAACATTTCACATGCTAAATCAATCCGACCATTGTTTTGTTGAATTAAAGCATGTGGCATTGAGCCAATTACCTTACATTCATAATTAATATCTTTTAAAAATTCAACCTGCTTTTGCGTAACAAAGTATTGCATTCCACCAACAGCAGCAGCATACCCATCATATGGTTGTAAACTATAATCATCAGAACGATCACTCATATAAATTACTTTTTGTTCATCATTAATTAAATTTAAAACATTATAGCAATTCGTTGCTATAGAACTACGTCGTGCTAGAATTGAGTCAATAATATTTTCTAATCAGCCAAAAATTTCATATGGCCCTTCGATAACAAATAAAGCTTCTTTTGGTTTAATAACATGTCCATCTGGAACATAATATATTTTTAATTGTTTTAGTTCTTTTTTAGATAAACAAAACTCTAATAATGCTAAAACCTCACTTGTTCCACACACCATGATCGGTGTTTTGCTAAAGTGTGTAAATTGCATAATAACTGAATGATTTGGTGCATGCAATTCAATTATCTTGCATGTTTTAATAAAATAATGAGCAGTATAGGCACTATTTAATAAGTCTCGATCAAATTTAAAATCAATTAAACGTGTATTTGGAATAATTGGTCCCATAATTATTTTGAATTTTTTTCTTTCATAAACATATTATTAAAAGTATTGATAAAAATATAAATACAACTTGTAATAACTCAAGCTCATAACCCGCCTTTTAAAGCAACTCCAAATTCATCCACTGTTGCTTGTGTTAAAACAACAAAATTAGCGCGTGAATCTTGTTGTTTTAACAATAAAAGATGTTCTTTTAGTGATGAATATTGTATAGTTGTTGTAGTTGTTGCTCATATTCAAAAAATAATTGATAATACTAAAATTGCGCCCATAACGGATTGTCAAATAATTTCACTTTTATTTCATTCTTTTTCTAAATGAATCATTAAAATTCGTCAAACACTAAAAGCAATCGTAATAAATAATGAAAGCCCTGATCCAATGCCAATAGCATATTTTGATGCAATAGCTTCAAAACTTCCTATAATTGCTAAGGCAAATGTTAAAAAGGCAAAAGCAATAATTAAATAGTTCGCAATTTTGCGTTTTTTAATTGTTCCAAATAATAATTGAATCGTTGAACGATCTTTTTTTTCTTTTTTTAATCCCATAATTCTAACTTTTAAATCCTCCAAGATACCTAGAATATAAATAAATTATTTTTATTTATGATAAGTAATAATATAATTATTAATTATAACAAGTTTGATATTTTATTATCAAGCTGACTAAATAAGTTTTACGTTTAACTATTTTTTTATATAATTCAGCAATCAAGAGGATTTTTAAAATGAGTACAAAAGCATTTAACCCAAAAGACCGTGTTACCAAAACTGTCGTTTTAAGCGATTTTGATCAGCATCAAATTTATTCAAAAAAACATAAAAAAATGGACTTTAAAAAACGCGTTATTTTATCGATTTTTCTAATAATATTAACTTGCGGAGCAATTGTTTTATTAGTTTTTGTTATTACTGAATTATTTAGATTATTAAGTGCATAATTAATCTCTATTTTATGATTTTTAACAAAAAAACTAAACATTTATACATTCATATTCCATTTTGTAACTATATTTGTATATTTTCTGATTTTAAACGTATTTTAAAAACTCCTCAAACAAAAAAATTTTTTAAGAATTTTTTAGAAAATATTAAAATGCACATTAATAATTTTAAAATAAAGCAATTTAAAACAATCTACTTAGGCGGAGGTGTTCCAAATTGTTTAAACAATCAAGAATTAGAAATTTTATTAAAAACAATTTCTCCTTATGTTGATGATAATTGCGAATATACCATTGAATGTAATCCTGAATTAATAAATCAAACTCAAATTAATCTCTTCAAAAAATACAAAATTAATCGCATTAGTCTTAACGTTCAATCAATGAATAATGATGTGTTAAAACAAATGAATTTTATTCATACAAATCAAGATAGTAAAAAAGCGATTAATTTATTACATAAAAACGGATTGTATAATGTGTCTTGTGATTTTTTATTTTGCATATCAATCTTAACATTAAAAGACTTAGATGATGTTTTTAATTTTATTTTAAATCATAAAATTAATCATGTTAGTTTTTATAGTTTAGAAATTAAAGAACAGTCACTTTTAAAAAAGTACCATATTACTATCGATGAAGAAAAAGAAATTGAACAAATGAATTATATAATTAATAAATTTGCAGGATTAAATTTTCATCGTTATGAAGTATGTAATTGAACAAATAACTTCAGATATATTGCAAAACATAACTTAGCTTATTGACGAACTGAAGACTGGGCCGCAATAGGATGGGGAGCACATGGCTATGAAAAAAATATTGTATATTTTTTTGATGGATCTATAAAAAACCCTATATTAATTAAAAAAGTATTAACAAAACACGAATTATATCAACAAATTTTAATGATGGGTTTAAGGTTAAAAGATGGATTAAATCTAAATAAAAAAATTAATTATGAAGCTTATTCATATTTTAAAAATCAATTGAAACACGTTAGTATTAATAAAAAAAATCATCTTCTTATTGATGATTTAAATTTACTAAATTTAACTATTTTAGATATATTTTAATTAATAATAAACAAATCTAGTAAGAATTGTGTTGAACTTTTTAAGGATTCACTATTACTTAAACCAAAATATAAACCTAAAATCAAAATTAATAAAACAATAAAAATGATAGGAATTCAAATAAAATACATCAAAAAACGAATTGCCTCTATGTATTTAACTTCTCGTTTAGTTCAATACACTAAAATAAAATTATTAAATAATTTTACAAAACGATTGTGTTGTAATTCTCGTTGTTCACAACTAAATAAATTGATTGCCTTATTGTTATCTTCAATTAAATTTGTTTTTAATTTAATAATTTCCAATGTTTGACGTTCAATTAAATCTCATTCTTTAAATAATGCTAAATTATTTCTAATAATAAATTTACACATTATTTTATAAATTGGTTTATTAAGGTGATAAGATTGATAAAGAATTTGAATCAAATAAGGTATTAAAATAAAACAAGATGAACTTTGGTAATAATAATCAACTAAAGGCTGTTCTAAAATAATTTCTTTTTCTAAAATGTCATTAGCTATTTTTTTAACTTGATTTAATGGAATTTTAGAAATAATATCTCGGTTTTTGTTTAAAAAAACTTTTAAAATCATATAAATTTCTAAAATAATTTTTTTGTTTTGTTCATGAGTTTCTTTATTATTATCAATTCCATTAATAAATGTTTTTGATCACGCATCATAAAATGCTTTTTGGATTGGTTTTAGTTTGATTTGCAAATCAATTAAATTTCTAATTTGTTCCATATTTTTTTGATTAAACCATATAATGCTTACTTAAACGTGATTTTTTTAATTTTTGTGTTTTAATAATGATTTTTTTATAAATTTCATCAATATTACTAATATCGCCTTCTTCTTTGATTGCTTTAAAAACAAAACCACGTCCATTACTTTGTTTTGGAATGATGTGTAAATGTGTGTGCATGATTACTTGACCAGCACTAGAATTTGAATTCATTAAATAATTAAAACCTAAAACATTAGGAAAAACTTCTTCTAAAACAAATGTCATATTTTTTGCTAAACGCATCATACCATCTAGATAAATAGGATCAGTTAGTGAAAAATTGCGATAATGTTTTTTAGGAATAATCAACATATGACCATCACTTGCAGGTTGAATGTCTAAAATTGCTATAGCAAATTCGTTTTCATCTATTATTTTTGAAATAATTTCACCTTTTAAAATTTTACAAAAAATGCAATCTTGCATCACTATCCTCCACTTTTCTTTTTATACCACTATTTAAAACTATTGCTTTCAATTTATTATAATCTACAACTAGAATAATTAATACTATAAAATTAATAATGAATAAAATTAAAAATAAAAATTGTAATAATAAAGTGCTTATAAGATATATTTTTTTTAATTTAGCTTCTTTAAAAATTTTATTAAAATCTTTAATTTGTTTTAACATTAGAATATGAGAAATAAAAGATGCAAAAATAAAAATTGTATAAATTACTATAAACAATACAAATCCAATTAAAGATGTGATGTTTAAAGCATTGGTAATTTCAGATTTATTTTTAAAGAAATCTAAAAAAACTTTTTTGTTAAGGAATTGAATTTCTTTGGGTTGTTTATAATCAATTATTTTAATTAGTGCACGAACAAAATTCCTTTTTTGTGTTAAAAGATCAAAATCAATCTTTTTAAAATCATCAATTAAATCAGGATTTTGTTGAATAATATCAATAATTTTTTGATTGTCCTCAAGATTTTTTATGTAGTCATAATTATTTAAAAAAACTTCAAATAATTGATTATTATTTTTAATAATATTAAACAAATTATCATCAACTATAAATTTATTTAAATCAACTGTTTTATAAAATTCTATTATTTGCTTTCATTCTTGTAAAGTTAAATTCTTAGTAAAATTATTAATTAATACTGAATATTTAATAGCCAACATTAAAATATTATTTTCTTTATCATTTTCTAAGCTTGTCTTTATAATATTAAATAAATTATCATCTACATGATAGAGATCTTTTAACATTTGAGGATTAGCTTTAATAACTTCTATATATTTTTCGTTAGTTTTTAAGTGATCAATTAAATTTTGGTGTTTTTTAATTAAATCTAATAATTCAGGTAAATTTTGTATCTGTTTAATTAATTCTTTATTATTTTGAACAAATTTTAAAATTTCAGGTTGCTTGCTAAGAATTTCAGCAAATGGCTTATATTCAACAATTTGTTTTAAAAATTCGCGGTTATCATTAAGTGTTTTTAAGATCTGTGGATTGTTTTTAATAACATCTAAAATTTCAGGTTCGTTTTGTAAATCTTTAATCAAATTCTTATTTTTAATAATAAAATCAAAAATTTCTTCTAAATTTTTAAAAGGTTTGATCTGAATATTTGTTAAATATTTTAATTGTGGACTAGCATCATAAATCTTTTTTTTAATAAAATCAATAATTGTTTGAACATTACCTGAAATAATTCATAAAATCAAAAAAGTAATTAATATTAAAACAAAAAAGAAAATAAAAATTTGAAAGTTTAAATGACGTTTCCTTTTTTTAAAGATTTGATCAAACATAAACATCTCCTGTCTATAAATAAATAATAATGTAAAAAATTATTTTTATAATTTTAAACTAATTTACATAAATATTTGTTTTTTAAGGCAACAATAGCGGGAATTGAAATTATATATAATACAAAATTAATTGCCATTGAACCAAAAGTTACCAAAGAATTATAAATCATAGAAGGAATAAAAGCAGTATTGTAAAAAAGTGTTCCTTGAATACTATAGCCCATTCAAACAATAAAATAACCAATGATTGAAAAGCTAAAAATTAATAAATAGGATAAAAATATTTGCTTTTTAATTGACTGACATGACTTTTTAACAATAATTAAAATGACATCCGTAAATAAAATTAAACCAAAACCATAATGTGGCATTAAATATCCAAAGAAGGGCTCAGCACTAAAACCAATTGCTAACATCACTAACGGCGCAATAATATAATACATTAATTTATAATAAAAATTGGGAATACAAACGATTGCTAAAACAAAAATTCCCATTTGCAATTGTCAACTAAAACCATTTAAAAAATCTAAATTTTTAGTTAAAAAACGACCAATTAAAAATAATGCAAGAAAAAATGCTGTTAAAACTAAATTAAAAATAATCAATTTTACACTTTGAGACCTAAAATTAAAATATCTTTTTAATGTTTCTTTAGTAGAAATTGATTGATCGTTATCTTTTTTAAAATACTGAAAATGAAATTTTTTGTTTTTATTGATCATAATAATAAATTATAAATAATTAAGATAAGATATATAAAATTTTACTAAATTATTAACTTTTTAAATCTTTATTATTTATACAAAGAAAAAACTTAATGTTTTTTTATAACATTAAGTTTTTTTAATTTTAATGGGGCGATTAACGGGACTCGAACCCGCGAAAATACCTGAGCCACAATCAGGCGTGTTAACCAACTTCACCATAACCGCCATATAACCATATAATTATAACATTATAGTTATCTTTTTATTGATTATTTTTAAGAAAACGAAAATTGACTTTTTTAATACGCTCGCCCTGCATAACAAATTTTTTTTCATATTCGGTAGGAATATTTTTTAATAATTCAACATTGTTAACAAGTGTGTATAAATTATAAGTTAAAAATAAAATTTCATACTTTGTTAAATCCTTTAGCAATACATCATAAATAGTATACATAAATAAATTTTCATTATCTGTTTTAAATTCAACAAGAGCATTTTGTTTTAAAATAACTGCAAACTTCTCTAAAAAACACGGATGTGTCAGTCGTTTTTTTGCATGACGTTTTTTAGGTCAGGGGTCAGAAAAATTTAAATAAATTTTATCTACACTTTCAGAATTTAATAATTCTAAAATTTTATTTGCGTCAAAACAAATCATTGCTAAATTATCTAATTTATGTTTTAAAGCTTTTTTGTGAGCTTTAACAATTACTGTTGGAAATTTTTCAATACCTAAATAATTAATTTGTTGGTTTCGTAAGGCATTTTCAATAATAAAATCACCCTTACCCATTCCAATTTCAATATAAAGAGGCAATTCAGGATTTTTAAAAATTTTTCCTAAATTATCTTTTAAAAGGTGTGGATCATTAATTATATATTCGTGTTGTGATTTTAAATAAAGTGGAGCATTGGCATTATTTCGTAAACGCATAAAAATTACTCAAATTTCTTATGAGGTTCATATTTGAATAAATCTTTAAAATTATTTTGCCGCACTACTTCATAGATTCCCATAGCAACACTATTAGCAATATTTAAACTTCTCAAATTAATGCTTGTAGGAATACGGATCCAATTATGAGGATATTTTTTTAAAATATTATGATCTATCCCTGTTGATTCTTTACCAAAAACTAAATATACATTATGTTGAATTATATTTTGATATTGATAATCGCACGGAGTTTTAGTACCATATCTAGTAAAAAAATATATTTCATCACCACTAATGTTGTTTTTATTAATAAATTCATTAAAATCATCATATTCATATAATTTTAAGGCATTTAAATGATTGGTTGAGGCACGTATAAAATCTTTGTGAAAACGTTTTTTATCTAAAATAAATCCATATGGTCTAACAAGATGTAAATTTGCATCAAAACCTACACACATACGAGCGATATTACCAGTGTTTAAAATAATTTCTGGTTCAAATAAAACAATATTTAACATAACATTATTTCACCTTTTGTTTTAAGTCAGTAAGAGTTTTTAATAAATTTGCGTGTTCTTTTTTTAAATTAATTAATTTATTTTTTTCTTTAATTATTAATTGAGCAGGGGCTTTGTTGATAAAACCTGAATTATTTAATAAATTAGTTGCTCGTTGAATTTCGTTTTCCAAATAGTTTAACTGTTCACTAGCTTTTTTCATTTGAAAAACATAATCAATCGGTTTTTCATACGAAGTTTTAAGAATAAAATCATCAATAACTAGCTCATAAGTATAATTATTAGAACTTGTTTTTTTAATTTCATTTATTTCAGCATTAACTAAGATTAATAACAAATTAACTAATTTTATCTTTGATTTATAAAATGCATCATTGGGTGATATTAATTCAAAACAAAGATTGATTCTATTATTAATATTTTCTTCTAATCGTAGCTTTCTAATTTCTAAAATAATTTTCGCAATCTTATCTATAATTATTGTTTCAAACTCAAAATTAATTATTGGATATTCTTGTAATAATATTGAATTATTTGTTTGATTTAAACATAATCAAATTTCTTCGCTAATATTAGGAGCAAAAGGCTGTAGCATGATTAAAATTTTATTAAATACATAGCGAATTGTTTTAATTGTTTCATTTTTTAATGTTAAATCTTGTAATAAAACTTTTGTGTACTCTAAATAATAACTCGCAAAAGTATTTCAGATAAAATCATATAAATATTTTGTAGCAACAACTAGATTATATTTATCCATATTTTTATTAATTTCCAAGGTTAATTTTGATAATTCAGTCAATATTCATTTATTGACGTCACAAAATTTATCAACATCTTCAAAATTAAGATTATCGTTAATTTCATCTAAATTTTCAATATATTTAGCAATATTTCAAATTTTATTTAAATAATTTCAATTAGCGCCTAATCGTTCTGTTGAAAAATTTAAATCTTCACCAATTGTTGTACATGATGCAAAAAATAAACGCATAGCATCTAATCCATATTTATCAATAATGTCAAAAGGATCAATACCATTCCCTAATGATTTTGACATCTTACGTCCCTGTGAATCACGAATTAAACCATGAATTAAAACATCCTTAAAAGGTTTTTTTTCAACCAAATATTGGCTAAAGTTCATCATTCTTGAGACTCAAAAAAATAAAATATCCATACCTGTAACTATTAAAGATGTAGGGAAATAACGGTCAAAAAAATGACTATTATAATTTCATTTTGTCGTTAATAAAGGTCACATTCCACTTGAAAACCAAGTATCTAAAACATCTTCATCCTGAATTCAATTTTCTAAATCCTTTGGGGGAATAGTGTCACAATAAATTTGATTAGTTTTTTTATGATATCAAACAGGTATCTGATGACCTCATCATAATTGCCTTGAAATACATCAATCTTCTGTATTTTTTAATCATGTAAGTAAAGTCTTATTAAAACGATTTGGATAAAAATCTACTTTATTATTACAATCATCTTGCATTTTGATTGTATCTTTTATTAAACTATCCATTTTAATAAATCATTGATGTGATAAATAAGGTTCAACAATTTCATTAGTTCTCTCACTAAAACCAACGCTGGTTCGATAAGATTCTACTTTTAATAAATGATTATTTTTCTGTAATTTATCAATTATTAAAGAACGAGCTTCTAATCGATCTAAACCCTTAAATTCCCCACATTTTTCATTTAAAGTACCATCAGCGTTCATAATATTAATAATTTCTAAATTATGTTTTTTAGCTAATTCATAGTCATTAAAATCGTGAGCAGGCGTACACTTCATCACTCCTGTTCCAAATTCAATATCAATATAATCATCCAAAATAACAGTCATTTCTATATTATTAATTGGATTAATAAAAATTTTATTATGTAAATGAAAATAACGTTTATCTTTAGGATTTATAACTAAATATTTATCACCAAACATAGTTTCAGGGCGTGAAGTGGCTACAACAACAAAATCCTTTTGATCTTGACTTAAATATTTTATATAATACAGTTTTTGTTCAACTTCTTTATGAATCACTTCAATATTACTAATTGCCGTTTTTAATTGAATATCTCAATTAACTAATTTTTTATCACGATAAATAATATGTTCATTAAACATTTTTACAAAAACTTCGCGCACTGTTTGAACAACTATTGGATCTAATGTAAATAAATAATTACTATAATCTAAACCCAAACCTAAAGCATTTCATTGTTTATGAATATAATTAATTTGTTCATCTTTTCAAATTTTTAACTTTTCTAAAAACAGTTCTCGCCCTAAATTGAAACGATTGGTTTGTGCGTTTACTTTTAAATTTTTTTCAAATTTTGTCTGTGTTGCGATCCCTGCATGGTCTGTCCCAGGAATAATAAAGGAATCATATCCTTTTAGTTTCTTATAACGCATTAAAATATCAGATAAGGTAAAATCATAGGCATGTCCAATATGTAAATGACCTGTAACATTAGGAGGGGGCAAAACAATTGTGAAAGGTTTTTTTGTACTATTTATTTGAGCTTTAAATAAATTATTTTCTTGTCAAAACAATAATTTATTAGCTTCAACCTCTTTGAATGAATACTTCTTACTTAATTTCTTCTTCACTACTCATCTTCTCCTCTTTTTTTGAAAGTATTTTTTCTTGTTTTGCTTGGATTACAACTTTTTTCATTAACGCAAATAATGTGATAATATTTATATTATTTTGTGCAGAAACACATAATAAACGATCGGTGCTAATATTTAAAAATTTAGCTATTTTATGCTTGTTATTATTAAAATATGATTTATTAACTTTATCTAATTTATTTAAAACAACAAAATGAGCGTATTTTTGATTCTCAAAATATCGACTCATTTCTACATCATCATTTGTTAATACGTTAATATCGCAAATTTGAAAAACAGCACACAAGTTTTGGCGATACCCTAAATATTGATCAATAATGGCTGCTAAATCCGTTTGTTTTTCTTTACTAACTTTTGCAAACCCATATCCCGGTAAATCAACCAATCTAAAATTATTGAAATCAAAAAAATTTACTAACTGTGTTCTTCCAGGCGTATTTGAAGTCCTTGCAATTTTTTTATTTGCTAATGCATTAATTAAACTTGACTTACCGACATTTGATCTACCAATTACACATATTTCAAACTGCTTATCAATTGGATATTGGTCAAAATAGTGTGCAGATTTAATAAATTTTGCCATATATTTATTTTTGTTTTACTGCCCGATTATAAATCTCATTAATTTGTGACTCACTTGGCTTACGTCCTACTTGTTGATAAATCATACGAATTGTATCCTTTGAAATTGGTGGATTCTTCTTCAATTGGCGTTTCATAATTTTCATCGAAATAAAATAACCGATAATAAGTCCTGCGATTAAAAATAAAACAATTCCAATACCTAAGCCAACTGCTAATGAATGTGCATGTGCATTAGCATTATCTAAATCTGCTTTAGTAACAACATCTGAAGCTTCATGAATAACTGATTTAAAAACATCAGAAACTTTAAATATAAAACTTGAAAAATTCATAACTACCCCTAAAAAAAATTACTTTTTATTTACTTAATTGACATTATTAAATATAATATTTAGTCATACAGTTATATATTATAATAAATGACTAAGTTAAATTTTAATAATTTTCTTATAGTTTAATTTAAAAACCAATGGAGGTTTTAAGTGAAAAAAAATAATGAAAATCAAACCGACTCCTTTATGTCATTTGATCCAGAAAATATAAATCCATCAACAGGTCTAAATGATGAGCAGGTTTTAAAAAGTCGTCAAATTTATGGTTTTAATGAAATCAAAAAGAAAAAAAAATCCCACATTATTACAAAATTTTTAAAGCAGTTTTTAGATTTTATGGTTATTTTACTTGTAATCGCTGCTGCTGTTACTTTAGCTTTAGTGATTATTAAACCTCCTCATGATACAGCTGAATTAGTTGTTCAATATGTAGAATTTGGAATAATATGTTTTATTTTATTGTTAAATGCAATTTTTGGTACCATTCAAGAAGTGAAAGCTGAAAAAAATACGGATGCATTGTCTAAATTAGCGTCTCATCAGGCCAAAGTATTACGTAATAATCAAATTAGAATTATTAATTCTAACCAAGTTGTAATGGGTGATGTTTTGATTTTAGAAGCTGGTGATCAAGTCCCAGCTGATGCTTTATTAGTGAACAGCTCATCACTTGAAGTAGATGAAGCTATTTTAACAGGAGAATCTTTACCAGTTGTAAAAAATGCCAAGGCAATTGTTAAAAAAAATACAGTAATTGGCGACTGTTTAAATCAAATTTTTTCTGGAACATATATTACTAATGGAACAGCAAAAGCAATTGTTACTAATATCGGTATGAATACTGAAATTGGTAAAATAGCCAAATTAATTAATGATCAAAAAACTAAATTAACGCCTTTACAAAAAAAAATTAATAAGTTATCTAAAATTATAGGTCTATTTGCTTCTATTTTATGTATTGCAGTATTTATTATTTATATTTATTTAATTGGAGATGGAAATTGAAATATTAGTTGACATCCCGCTTTAATAATGGCTATTTCATTATCTATAGCAGCTATTCCTGAAGGAATTGTGGCAATTGTAACAATTGTTTTATCATTCGGTATTAAAGAAATGGCAAAACAAAATGCTTTAATTAAACGTTTGCCAGCTGTTGAAACTCTAGGAAGTGCTAATGTTATTTGTTCTGATAAAACAGGAACCTTAACACAAAACAAAATGACTGTTACTAAAGTTTTCACTAATAAATTAAAAATAACAGATTTAATTGATAATGAGAGTGTTTTTAATTTAATTAAATGAGCTACTATAGCAAATAATGGAATTAAAAATTTTAATGAAAAAAAACAAGAATATGAGTTTATTGGCGACCCTACAGAAACTTGTATCATTGAAGCTGCTTTAAAATTAAACATAGATAAATATAAATTAGATCAAGAATTTATTAGAATTCATGAATTTCCATTTGATTCAACTCGAAAATTAATGAGTGTTGTTGTAAAACATCAAAGTGATTATTATTTAATTACTAAAGGAGCAATTGATGCTATTGACAAAATTGTTCAAAATCCAATTACAAAAGATGTTTATGAAGCAAATGATTTTTTAAGTAAACAAGCTTTAAGAATTTTATGTGTTGCAATTAAAAAACTATCATCTATACCAACTAATTTTAACCAAGATGAACTTGAAAAATGTTTAGAATTTGTTGGTCTAATCGGTATGATAGATCCGCCTCGTCCTGAAACACAAGAGGCAGTAAAAATTGCTATTAAAGCTGGAATCCGTCCGATAATGATTACTGGCGATCATATTAATACAGCATCAGCCATTGCTAAACAAGTTGGTATTTTAAACGAAGGACAAGAAGTTTTGAATGGTAATGAATTGAATATGATGAGTGATAATGAATTAATAAATAATATTGATCGTTATAGTGTTTATGCCCGAGTTTCTCCAACAGATAAAATCCGTATTGTTAAAGCTTGACAGCACCATGATAAAGTAGTAAGTATGACTGGAGATGGTGTCAATGATGCACCTGCACTAAAAGCTGCTGACATTGGATGCGCAATGGGTATAACTGGAACTGATGTTTCAAAAGCATCAAGTGATATGATATTAATTGATGATAATTTCGCAACAATTATTAATGCTGTTAGTTTAGGACGTTCTATTATGGACAACATTAAACGTATTATTGTTTTATTATTGATCACTAATTTATCTGGATTAGTTTCATTAATATTTGGAATTATTATTCTAGGAATTAATCCTATGTCATCACTACAAATATTATGAATTAATGTAATTGCTGAAACTTTACCTGGAATAGCATTAGGGTTACATTTAGCGGATGTTAATTTAATGCGACACAAACCATTAAAAAAATCAGCACCGATTGTTAATAAAAGAATGTGAGCTACTATTTTTACCAATGGTTTTTTTGTTGGTTTAATTTCTATTTTATTATTTTATTTAGGAGCTTCTTCACACTTTAATTTTGACTTTATGCTAATGAGAAACGAGTTTAAAGAACTTACTAATCTTGAAAAGATATATCAAGACACATGAAGTTGATTTGGGCAAAATCACGAAATTTCCAATATTATTCATGAAAAAATTTTAACCATAAAAACAGCGATTATGGCTGGTTCGTCTTTAACATTTGTTTTTATGGGAATGAGCTTAACGTTTAATGCATTAAGCTTAAGAACAAATCATTCAGTATTCGTTAATTTTTGAGAAAACTGTAAATACGTTGTTTATTCAATATTAATATCAATAGTTATGATTATTGTTATCGCTTATACTCCTCATTTAAATCAAATATTCAATATGAATCCATACAACATAAACGGTTATGAGTGATTTAATATTCTTCCATTTGTTCTTTTTGCAATACCTTTAAGTATTTTTGAAATTATCAAATATTTTAGATATTTAAAACTACGTAGTACTTTTGATTATAAAAATAAAACTTATGCATTATTAAACCAAGAAATACAATCAACAAATTTAAAAATTAATAATACAAAAATTAGTTATGAAAAACAGTATTACAAGGCATTGTTGAATAATTTAATTATTAAAAGAAAAATTTTAATAAATAAATGCCATAAAGAAATATAAAAATAAACCTAACTAACTTTAATAATAAAGTTAGTTTTTTAAAAAGGTAAATGATTTTATTAAATTCATACCTAACTTTAAATAAACATTTAAATTGAATTTTTGTGTAGAAATATAATATAATTCATATTAGTCAATATCTGTATTTATATAAAGAACTAATATATATATATATATATATGATATTAAAACTAATGGATATTTTACATTATGAAAATAAATTTTAAAAGAACTTTTTTATTATCATTTATAGGAGTTATAGTTACACCAATTCCTATAATTTTGAGTTGCTCATTAACAAAAATTAAAAAAGAAGACAATAAAAATTTCTTTAATCTTTATTTGAAAATTAAAGAAGAAGCTAGTTCTAAAGTTGATTTTGTTCTTAAAAATAATCATAAATACAATTATCTTCTTAATAAAGCAGATATAATCAATAATTATATTAATTACTTGGTGAATTTAAATTTAAATGAAAATAGTGAGAATGAATTATTAGCATTTACTTATTATGAATTATATAAATTAATAAAAGATTTTAATTATCTTTTAAAAGAAAAAACACAAGAATATGAATCATGATTAATTAAAATTGATAACTTTGTTAAATTAATAAAAAAAAGACAAGCCGATTTTTTAAATTTTAAAAATAAAAACAAAATTATTCATGATATCGATCATATCAAAAATAATATTGAAGAAATTTTAACTAATTATAATCAAATTCCTGATAATGAAAATATTGATGATATCAAATTAAGATATAAAAAAATGATTGAATCATACGATGGTCTTTATAAAAAAACAATTAATGATGAAGCTAATAAAATCAAAAATATTAAATCTGATGTTAAAAATTTAAAACATAATTATTTTGAAAAAATTGATAATGGTGAAGAATATTATAAGTTATACAATAGAGAAATTATGGAATTATTTCATGAAGCTAATCAACTTCATGAAAATATTTCTTCAGTACTACAGAGTATATTAAACAGAAAATTAATTGAAAATAAAATCGATAATATTAAAAGAGAAATCCAAGAACATAAAAAAGTTTCACAATATATTAAACAAAATGGCCACAAAAATAATCAAGATTTTTGAGATACATGAAAAGAAGATATTTTAGGTAAAAAGTTTATATATCAAACTTTAATGGATAATACTAATGTTGCTATTTGATATGGATTAGCATTAAAAATTGAAGATTTAGCAAAACAAAAATTTATAAACACTAAATCTCTAGAATTAAAACAATTAATAGAAAAATGAAAATTATATGGTCAAGCACTTATTAGAGATAGTTTAGACGCAGCCAATTTTTCGGGTCTATTTTTTACTATTAAGTCCAAAACTTCAATGTTAGATAATTGACAACCATCAATAGATCCTAATTCTCTTTCAAAAAAAGGTAAATACTACCTCTTAATTAAAAAATTAAAAGATTTAACAAACGAAATTGAGAAAAAAGCACGTAATGCTAATCAAAAACTTTCTAGATTTTATGATAGCGTTGTCAAAGATGATGTTGAGCAAATTGTTCCATATATTAAAGCCGTTGGTTATGGTTTTTATCCCCAAGAAGAATTTGGTGAATTAATGTATTGATCTAATTACTATTTAAATCAATTACCAGATGTTTTTCAATATTATAAAGAAAAGATAATTAAACCTATTATTGATCAAATTAAATCAAAAAGTGATTCAGCAAACGATGTTTCAAAATTAGAAGAACTTTATAATTTTAAATATCATTACACTAATATCTATGAACCTTTTAATCAAAAAAGAGATAATTTTTATTATTATTCTGACTATAAAACAGATGATAAAGGTGAAAAAATTAAAAATAGTGATGAAATGTTTATATTAACAAAACAAGTTCCTAATCATTTAAAAAATGAATATGATACAGCAAGATTGTTTTTTAGAATAGAGAGAACAATTGGTGAAATATGTAAAAAATACGCGTTAACTAATCCTTTAGAGTAAAACATTATTAATAATCATGTTTTATAAAAATAAATTCAATCTTAGACATAAATATATTAATTTTGGTGTTAATTCGGATTTTTAAAATAACTCTATCATCTTCATAACTCTCTATTAGTTTTTCATAACTAATTGATGCTAAAATTTGCTTTGCAAATTTAGTATTTTTTAGCTTATAAACAACTTTATAGTAAAAATATTTTTGTAAAATTATTATTTCACAGTTATCTATAATTTCTTTAGCACATTGTGAATAAGCACGAATTAATCCTCCAGTGCCTAATTTAATGCCACCAAAATAACGTACAACAAATATAATCACATTGGTTAATGATTTGATCTTAATGATATTTAAAATAGGTATTCCAGCTGTTCCATTTGGTTCACCATCATCATATGCTTTCATATAATTAAATTCACGACCTATCACATAAGCAAAACAAATGTGCGTTGCATCGTAATATTGTTCTTTATATTTTTTAAATAATGATTGAACTTCATTTTTATCGCTAATAGGTTCAACGATCGTTATAAATTTTGATTTTTTAATAATTTTTATATTTTTTTGGGTATATGCAATTGTTTTAAATGACATTTGTTTTTTTAAATTCTTCCAAATCTATGCAAATTTGTTGGACTTCTATAATTGTTAAGTTTAAATATAATTGTTTATTTAAAATAAAATCAAGAATATTAATAATATACTTATAAGATAATTCTTTTTGATTACGTAGTCATTTTAATAAATTTAAACTATAATTATTGATAATTTTTATATGAATACAAAAGACAACTAAAACACTAAAAATTAATACAACACCAACTAAACTAACCAAAGGATTAAAATAATTTGATAAACCAAATACTAATAAAAAAATAAAAATTATTAATGAAGTCAAAAAAGCAAATGCATATTTCTTTATAAAATATGTCTTATTTTTATAGGTTTTATCATTAACATAATCAATAACTTGCTGTATGTAATTGTTCATATTTTTATACTTCTTTTCTAATTAAATTATAATAAAAAGAAATAAAAATAACACTTCTAATTCATATTAATTAGGATCAATATCAACAAATGAAAAACGAATTGCTTTTCCGCCAAATTGATCTTCATAAACTTTAATTTCAAGAGGTGAATGAAAATTAACAGCATTGTAATCGTATGAATTTTCAGTATAATTAGCTAAATTAAATTCTTGTTTTATTACATGATCATTAAACATTAATCTTGGTAAAGTTTTAATTCATGGCAACAAACTAACTTTACGCGAAATTAGTTTTTTGGTTTTATCATAATTTTCTTCAATGGAGATTGTAAAAACAAAACCTTCATTATTCAACAGAGTAAATTTAGCTGTTTTACTAAAATCAGTATATTCATCTAATTCTTCTGGTTTTTTATTTAAATTAGAACTTTCTACAACATATAACCCATCAATAAATTCATTCGATTGAGCACGATAAAAAGAACTACGATTATTGATTTTTGAATCTTTTAATCCTTCTGCTGTTTCCTCGCTCGATTCTTGAAATAAATTATCATCAGGATATAACATGAAAATTTGTCTTTTTAAATTAAATAAAAATCACAATCAATTTTTATTAAATAATGTATTGTGAAACTTACGTGCTTGTTCATAAGCAAATGGATTGCTTAATGATACATTATTAAAAAGATTGTTATTAAGTTTACTTCTAGTTGTATTTTCATTAAATACTGCTGCTTTATTAGCATCATTTTGCACTGTGTTATAATAATTTAATCAATTCGCTATTTCATGAGCATATTCTATTTTAATTTCTTGTTGTGATTTAATATATTCATCACGGTCTTTTTTATTAGGAAAAATAGTTATTAAAAGATTATTAATCACTTTGTTATTTAAAAAAGCTATTCAAGAATCTTGTGTTTGATTATTTGTTCTGATTATATTCAAATCAATTAAATTATCAATATTAATTATTTTTCTAATAGGTATGAATGAAGCATTATTGCTTGTAATAATTAAATGATCATTAAGTTTTATTAAACGCGGTAATCTAATTAAAATTTTGTTATCTTTAATTTGATAATCTTTAACTAATAAATTTAACTTCGTATTTTTTTCATCAATTAAACTAACGTTAAAAATAGTTTTTTTATCATTTTTTAATGTCTGATCAACAATTAAACCAACATAACAATTTTGGGATTTATCCATAAAAAAACTAATTTCATTATTTGTTAATATCTGTTGTTTTTTTAAATTTGATGTGCTACATGAGGTGATAAGGATTGAAATTAATGTTAGAGGAATAAAAAATAAACTTAATTTTAGTAGATGATTTTTTTTAAAAAAAATAAACATTAATTACTATTCCCTTCATAAAAGTAACATTTGGGCTGGGATACCATATTCATATTTATCAATCATATCAGTTTCAAACAATTGATAGTATTCTTTATTTTGGTGAAATATAGCTTGATGAAAAATTGATGTGATTAAATCAACTGATAATTTATTTTTAGTTTTAGGAAAAAAATAAATCAAAGGATTTAAACTTAATCTGTTTTCACTACTTGAAAAATCGATCTTAATATTAAAAATAGATTGATTTTTTGTAATATATAAATCCTTAAATGAACCAATCAACATTGAAGTATCTCCTTCTTGTTTTTTTTCTAAATAATTATTAATGAAATAATAATCCTCATATGGTTTAAGTTTTTTTAACCTTTTTAAATATTTTTCACTTGGTCCTAACATCTCTTTAGTAATAGGATTTATTTGTTTTGGTAAAGTAAATCAATTATTAAAACTTAATTCAAAGTGCTTTAAATTCATTAAAATAAAATACCAATTTTGTGAAAATATTTTTTCATAATCATTTAATTTTTGCTTATATTCATTAGAATCTCTTGGTGTTTTTTGGTTTTTAAAATAATTACTAAATTCTATTAATTGTTTAATTAATTTATTTTGGTATTCCTTATCATTTTGTTGAATTAGATATTTTGTAATTTGAACATCATTATTATTGAAAGCATCTAATAAAAGATTTTTAATTATTTTTTGTGTAATATATGCTTCCTTTGAAATTTTATTAATATTAATATCTAAATTATGATCAATATATTTTTGAAAATAATTCAATTCTTTATCAAAAGATCTAAAATAATTCTTATTTTGTTGTTTTTTTTCATTGTCATTAAAATGATCACGTGCTCTAACTTCTTTTTGATCAATATTTCTTAAAACATAACGTTTAAAACCCAACTTATCTTTTAATTCTAAAAACATTTGATAACTAAATTTTTGAAAAAGTGGTTTTTTAGTATTAAAAAAATTAAAATTATTGACTTCATAATATTCTTTATTTATTTTATCTAATCCCTGTTGATAAATACTTGAGTTAAGCTTTTCATCATAATATAAATAATCTTCATAATTTTTTTTATAATCTAAAATATCTTTTTTAAAATTATCTTCTCGATGATAATCAATACTTGTTTGCATTTCTTTTGCTATTAATTCCAAATTTATAATTGGTTTATTGAATTGAAAAAGATCTGTATCAAATCGAACATCAAAATGATTTTTTTTATTAAATTTTAATAAACGTAAGATTAAGTGATCACCAAATTGCAAATAATAAACATCAACACTACGATAATTTTCTTTATCGAATAATTTTTGCTTAATTTTTATGTAATTTGTTAAGGGTTTATTAATATTAATAACTTTGTCAAAAATTGATGATTTAGTAAAATCTTGTTTTTCATTTATATAGGGGGCATAAAAATTAGGTTTATAAGGAGTGTAAGAAAATTCTAAACTCTTTAAATTGGTAAATAATCAGTATCAATTATTAGATAAACTTTGAAAAATAATTTTTTTAGCATTATTTTTCATAATGGCAATTGTATCGTCATCATTAATATCTAATCTGTTAGGATTTAATAATGTTAAAGCAAATTTTAAATCTAATAATTGTTGATCTGATACATTATTTTGATCATACATATAAGCATTTAATTCTAATTTATCTTGATTAAAAACATCTAAAAACAATTGTTTTGATAAATCATTAATAAAAAAATTGCTACTTTTTTGAACTTTAGAAGTGATGGAATTTTCATTATTTTTTATTAATCCCAAACCTAACCCAATACTTAAACTAATAATTAAAAATAATGATATTGAAGTTAATACTGAGATTTTTCTTTTTTGTTTATTCATTAGTCTATAAAACCTAACTTTTGTATATCTTAATAATAAAATATATTAAAAATATATGACCAAAATTACAAAATTACTTGTAAATATTATTGTTTATCATTTTTTTAAAAAAAGGTGGAACAAATCAATTAACATATAATAAAAAATAATTAAAATTTTATTTCGTTTTTTGTTTGTAAATAAATTAATTTATATTTTATATAGTAAAAAACCAGTTAAACGAAATGTAAAACTGGTTTTTATTGATCTATTTTATTAAATATTTACTAAACTAATTGTAAAACAGCTTCTTCTGTATTATCTCCTGGACGATTACCAAGTTTAATAACACGAGTGTATCCACCAGCACGATTAGCATACTTAGGACCTAATTCGTTAAATAATTTACGTAATAAATCATCCGCTGAGTGTTGATTTGTTCCTAATAAAATCGCAGCTGCTGCACGACGTGAAGCAAGTGTGTTTTTCTTAGCAAGAGTGATTAAATGATCAACATGACGTTGTGATTCTTTTGCTTTTGTTTTTGTTGTCACAATTGAACCATGACTTATAACATCAGATACTTGTTGACGACTCACCATTTTTCTTCATGCGCGTGTTTTACCTGGTTTGTTAATGTAACTCATATCAAATTATTCCTTTCTTTTAAAGTTTTAATCTTTTTTTAACTCAAGTGAACGTTCAGTTAATTTTTGAATAATTTCAGTAACTGATTTACGTCCTAAATTCTTAATTTTTTCAAGTTGTGATTTTGTTAATTCAATTAATTCAGCAGTGGTATTAATACCTGATTGTTTTAAAGCATTATATGCACGCACCGTTAAATCTAAATCATCAATTGAAATTGAAGCGTTTTGACGTTTTTCTTCTTCCTCACGCTCACGCATAATAATCATTTCATTAATTGTTTTATCAATGTTCACGATTGGTTCTAAATGCGCTTGCATAATCTTAGCAGCAATCGCAATTGCTTCGCTTCCTGATATCGTACCATTTGTTGCAATGTCAATTGTTAAAATCTCAGACATTGATGATTTTGAATCTTTAATCATTTCTACATTATAAGCGTAATGTAGAACTGGTGAATAATTTGAATCGGTTGCGATAATATGTAGTGTTGAAACAAAATCCTTATTTGTATTAAAATCGACACGACCACGTCCTGTTTTTGCAAAAATTTCAATTTCTACATCAATTGGTTTTGTTACTTCTGCAATATATAAGTCTTGGTTAACAATTTCAAAACCTACTGGACATACAATATCTTTAGCTGTAATCACACCAGGTCCTTTGAAATTAATTTTCATTTTTGGTCATTTATCAATTAATAAATTGTTGAAATAGTCTTCACTATAAATTAATTCATCAATTTTAATTGCTAAATTTTTTAAGTTTAAAATGATGTGAGTAATGTCTTCTTTAACACCTTCGTATGGTTGAAATTCATGCGAATATCCCCCAAATTTAACAGCGAACATTGAGGCACCAGGAATTGATGATAAACAAATTCGACGTAAGGCATTTCCTAATGTGTCACCAAAACCAATTTCAAGTGGGGCAATTTTAACAACTGTACGATTTTTGTCTTCGCTATTAATTGATGGCACATCAAGTTGGTATTTTAAAAATTTACGCATGTTATTTCTCCTTTTGCAATAATCAAAACCAAATTACTATCTTGGTTTTTTAGGTGGACGACACCCATTATGTGGAATTGGAGTAACATCTTTAATTTTTGTTACTTCTAAACCTGCTGCTTGTAGGCTACGAATTGCTGTATCACGACTTGCACCTGTTCCATTAACATGAACTTCAACAGATTTTAATCCCATATCAATCACAGCTTTTGCTGCAGCTTCAGCAGCAATACCTGCTGAATATGGAGTTTTTTTCTTTGTTCCTTTATAACCGATTGAACCAGATGATGCTCAAGATAAAACGCTTCCTTGTTCATCAGCCAAAGTAATAATCGTATTATTTTTAGTTGCATGAATATATGCAATTCCATTAGTAAAACTAAGTTTTTTCTTCTTTGCCATAGTTTGATTATTCCTTTCTTATTATCTAGTTTCAATCTTTTTATTAGCAACTGTTTTACGTGGTCCTTTACGTGTACGAGCGTTTGTTTTTGTTCTTTGTCCACGCACTGGTAAACCACGACGGTGACGGATTCCACGATATGAACCGATTTCCATTAAACGCTTAATATCCATGGCTACAGTTCTTCGTAAATCACCTTCTAATTGTAATTTTTCGCCATTTGCTTTAACAAAATTTAAAGCAACTCGACGAATTTCAGCAATTTGTTCATCAGCTAAATCCTTTACACGAATATTTTCATCTATATTAGCTAATTTTAAAATTTGTTGAGATGTAGATTTTCCAATACCAAAAATGTATGTTAATGAAATTACAACTCTTTTATCATTAGGAATATCTACCCCTAAAATACGTGCCATATTATTTATCTTCCTTTCTTTTTATTAACCTTGACGTTGTTTGTGTTTTGAATTAGCACAAATAACACGCACAACACCTGATCGCTTTACGATTTTGCAATCTTTACAAATTGCTTTAACTGAAGCTCTAACTTTCATTATAGTTTTCCTTTCGTTTAATTTTAGTTATCACGATGACGATATGTAATTCTTCCACGTGTTAGGTCGTATGGACTTAACTCAACATCAACAGTATCACCTGGTAAAATGTTAATTTTATTAACACGCATTTTACCTGATACGTGGCTCATAATCGTGATGCCATTTTCTAATTGAACTCTAAAATTTCCGCCTTGTAAAACTTCAACAATTTTACCAAGCATTTTTAATTTTTCTGTATCTGCCATTTTTATTCCTTGTTTAAAATATATCCCTTAATCTGTTAAAACTTCACAACCATCTTTTGTAATTAGAATCATATGTTCTCAATGACAAGTCATTTGATGATTTTTAGATTTTACAGATCATTGATCGTTTGGATCAATATAATAAGCATTTGATCCACTTAGAATCATTGGTTCAATACAAATTACCATATTCTCTTCTAATGTAACATTACGATACAATAAAGATCGATAATTAGGAATCAATGGATCTTCATGAATTTCATTACCACAACCGTGGCCGGCAAAATCACGAAGAACAAAATAACCGTTTTTTTCAATAAACTTTTGAATTGCATTCGAAATTGCATGTGTTGTTACTTTTGGTTTAATAATTGCAACGGCTTCGTCAATTGATTTTTTACAAATCTCTGATATTTTTAGTGCTTCAACATTTGAGTTGTTAATAATAATGGTAAACGCCGCATCACAATAATGATTATCGTATTTAACACCAACATCAAATGTTACCTTATCACCATCTTTTAAGACGTAATCGGTTGGGACTCCATGAATAACGCACTCATTCACTGAAATACAAATATGACCTTTAAAACCTAAATAGTTGTGAAAAGCAGCAATTCCACCATTTGCTTCAATAACTTCTTTAGCTAATAAGTCTAATTCTTTTAATGAAACGCCTGCTTTAGCTTGTTTATAAATTGCTTCACGAGCAATTTTTCAAATTCGCACAGCTTCTTTAATAGCAGCAATATCTTTTTCTGTTTTAACAATAACCATGGTTTTAACTTCATCTACTTTTTTTTACTTATTTTGTTTTAAATAATTGTTAATGTTTTTATAAACATCTTCAATAGGTTGATTAGCATTGATTTTAATAAAACGTGGATTAGTTTCATAATGCTTAATCAAAGGTAGGGTTAATTTATCATATTCATCTAATCGAATACTTACAGCATCTGCATGATCATCAGCTCGTTGATAAAGAGTAGCTTGATCATTGTCACATAAACCATGAACTTGTGGTTTTTTAAAATGAATATTATAAATTCCTGCACATTTTGGGCACATTCAACGCCCAGTTAAACGTTTCATTAATTCTTGATGATCAATATCTAGATAAAAAATATAATCTAAATTACAATATTGTTCTAATGCTAAGGCTTGATTAATTGTGCGTGGATAACCATCTAAAATAAATGATTGTTCATTTTTAATTAATTCATCAATTGCTGATTTAGCAACTTGATTAGTAATTTCATCTGGTACTAATTTACCACCAGAAACATTAATCTCTTTAATTTTACGTGCTAATTCGCTATCCTCTTTTAGAATTGCACGAAATAAATTACCTGTGGAAACATGTTTTAATGTATTATTTTTAGTTAATAATTCACTAACACTTCCCTTACCACTCCCTGGGGGGCCAATTAAAAGGATTTTCATAAATACCTCTATCAAATTTGTGACTTACTACTCTTGTCGCTTTCAGCGCTTACTGTAATGGATGTTTCTAATTCTTTTCTTTGGTAAGCGTATGATGTTGAAGTTGATGTTGATTTAATTGCATTTCATAATTCAAGCGTTGCTGTAACCATAATAATAATTGAAGTTCCTCCAAAAATTGTTGATGGTTGAATTACAGGAATTCCATGATTGATTGTTTTAGCTACTAAAGCAACCAAATGAGGCAAGCATGCTAGAAAAGCAAGAATTGGTGCACCAATTCAATTCACGCGATAAATTACTTTTGTAATATGTTTTTCAGTATCCATTCCAACTTGAACACCTGGAATAAAACGACCGGCTTTTTTAATATCTTCAGCCATTTTTGGTGGATTAAGTTGTACATATGAAAAGAAGAATGTAAATAATAAAATTAAAACAACATAAATTGCTAGACCCGTTCAAGAATCAACAATGAAGTAATTATGAATCACATAATAACCCATGCTTTGTTTATCTAAAAATTCAGCAACTTGTGCAGGAATGGCTAAAACAGATGATGCAAAGATCACTGGCATTACTCCTGCTGTCATAATTTTAATAGGAAGAAATGGTAGTTTCTCATGATCTAAAATTAGAGCTTGACCGGTTTGTTGAACTGGAATTTTTCTTGTTGAATTATTAACAAAAACAACACCAATTAAGATCATAATATAGAATAAAATGTATAAAATGAAACCGATAATTTGTGAAACTTTACTGCTTGTTAAACTACCTAAATTTGAAAAAATTGCAATAAAGTGATTAAATAATGAAGCAACAATTCCTGTAAGAATTAATAATGTAATTCCATTACCAACACCTTTTTTCGAAATTAGATCAGCTAAAAATAACGAAATATAAGTGCCACCAACCATTAAAAAAATGTATGTTACATAAAATAATGGTGAACCAATAGCAAATGGTGCATTTGAAACAATTGAAATAAATCCATTTGCTCTTGTCATTAAATTAATAATAATGACAGCTTGCATCACTGCAAGAGGCAAGGTAATGATTCTTGTAATCACTTCAATTTTTTTACGACCACGCTCACCTGATTTGGATAATTTGGCGAGTGGTGGCACAAGCTCACTTGATAAAAGTTGCATAATAATTTGAGCGGTAATATAAGGTCCTATCCCAATTGCGAAGAGTGAAAATTGTGATAGCCCTCCCCCGCCTAATAAGTTAATAATTGAAAAGAATGAACCTTGATTGCCAAAATTACCATTTAACTTAATGTATGGCATTGGGATAACAGATCCAATTCTAAACAAGATTAATATACTTAAAGTTACAATTAATGCAACTAAAACTTTTTTATTTTTAAAGATCATTAACAACTGTCTAAAAGCATTTTTTTTCTTCTGTTTGTTAGTCATTGTTAATCCCCTTATTTTGGTTTAGTAATAAATTATATATTATAAACTTTTATTACTCTAAAATTTGTGCTTTTGCTTTTGAATTACTAATTGATTCCAATGCGCCTTTACTAAATTTGTGAGCACTTACTACTGTGCCACTTGGTAAAACTGCATTACCTATAACCTTAATTGGTAAATCTTCATTTTTAGCAATTTTATTTGCATACATAAATTCAACTGTTAATTCTTTTGTTTCATATTTTACTAATGAAATTAAAGTTACAACGTTATATTTATTTGCAAAACGATCGTTGTTAAAACCAACTTTTGGCACACGACGATAAAGTGGTGTTTGTCCTCCTTCAAAAGCTAAACGTACCATTCCTGATTTACGTGCTTTTTGTCCATCTTGTCCACGCCCAGAAGTTTTTCCTAAACCAGAACCATGACCACGACCAACACGTTTTTCTTTGTGGTTTCTTGAACCTTTTTTATATTCTAAATTATGTAATTGCATGACAAACTCCTAATCACTATATTTCTCTTAAACTTTTGTCACGTAAACGTGCAATTTCTTTTGGTGTTAATTGTTGTAATAACCCATTGATTGTGGCACGAATAACATTCATTGGCGCATTAGCACCACGTGATTTTGTATAAATATCAGTAAATCCAGCTAATTCAACCACAGCACGTACAGGTCCACCAGCAATAATTCCAGTACCTTCAGGAGCAGGTAGTAACATTACTTTAGCAGCTCCATGACGACCATTTACATCATGGTAAATTGAACCTTTTTTTGTTTGCTTAACTTTAATTAAATTATTGTTTGCGTTTTTAATCGCTTTTTTGATTGCATCTGGAACTTCAATTGATTTACCCATACCAAAACCAACAGTTCCATTTTTATCACCAATAACAACTAAAGCACTAAAACGCATCATACGTCCACCTTTTGTTGTTTTTGAAATTCGTTTAATTTTCACTACACGTTCTTCAAAATTTGATGTTGTTGTTTCACGTTTAAATGTTTTTGCTTTTGGTTCTGTATTTTCTTTTTTATTAACTACTGGTGCAGTTACTAGTTGTTGCTTTTCAACTTTTTCACTATCTACAATAGTTTCTTTTTTAACATTGTTTTCCATATTATAACTGCTCCTTTTTAAAATTCTAATCCAGCTTCACGCGCTGCATCTGCTAATGCTTTAATACGCCCATGGTATTTAGCACCATTTTTGTTAAAAATAACTTGTTTAAAGCCTTGTGCTAAAGCTTTTTTAGCAATATCAACACCTACTAAACGTGCATTATCTTTATTACCATTTTGTAAATCTAATTGTACAGATGATGATGAAGCTAAAGTAATATTTTTGTTATAGCAAATTAATTGTGCTCAAATGTGTGCATTTGTTTTTGTAATAACTAAAACAGGTTTGTTTGCAGCTAATTGAAGGTTACGAATTTTAACATGAAGACGTTTAGCACGAAGTGCACGTTGTTTAGCACGACTAAAATTAATTCTTTTCATACTTTATGAATGACCTTTCTATTTTTTCTTATCTGCAGTTTTACCTGCTTTACGCACAATTCTTTCGCCCTTATACATCACACCTTTACCTTTGTAAGGTTCTGGTTTACGATAAGCACGTACTACTGCTGCAAAAGCACCAACAGCAGCTTTATCTGCTCCATTAATACTAATTTCAGTAGCTGATGGTGTTTCAACTTTTAAACCTGTTGGAATTTGAATTAATAAAGGGTGAGAAAAACCTAATCCTAAATTAAGTTTTGAACCTTCAACTTTTGCTCTAAAACCTACCCCTTTAACTTCTAATTCTTTCTTTCAACCAGTAGTTACACCAACTAAAGCATTAGCAATATTTGCATTTACTGTTCCATGAAACATTTTTGTTTGTTTTTCATCGTTTGCACGACTAACTTTAACAGTATTATCAACAACATCTACACTAATTAGATTTACTGGAAAATCAACACTTAATTTTGCAGTTTGACTAACAATATGTACTTTACCAGATTCAACACTAACATTTACATTAGCTGGAATAGTTAATTTACGATTTCCAATTCTTGACATAAATATTCTTATCAAACGTAAGCGATTACTTCCCCGCCTACATTTTCCTTTCTTGCTTGTTTATCTGACATAACACCTTTTGAAGTTGTGATGATAGCAATTCCTAAACCATTTAATACTTTTGGTAAATTTAAGTGTGTTGAATAAATTCTTAATCCTGGTTTTGAAATTTGTTTAAAACCATTGATAGAAGATGTTGTTTGGTTTTTGTATTTTAGTTTAACAACTGTTTCAGATTTATTGTTTTCAGTTGGACGAATATCATAAGATTTGATATATCCTTCTTTAACCAATAATTCTAAAATCGCTGTTTTAAGCTTTGAAGTTGCAAAACTAACTTCAGCTTTATGGGCCTTACGACCATTATTAATTTTTGTAATTAATTCAGCAATTGGATCTAAATACATATCTATCCTCCTATCAAGATGCTTTCTTAATTCCTGGAATTGCACCAGCGTAAGCTAAATCTCTAAAGCAAAGACGACATACACCAAATTTACGATTAACAGCATGTGGACGACCACATCTTACGCAACGAGTGTATTCACGAACTGCAAATTTTTGATGTTTTTTTTGCTTAGCAATTAATGATTTTTTCGCCATAAATTATTTGTCTCCTCTTACTTTTTGAAATGGAGCACCTAGTCCCACTAGTAATGCTTTAGCTTCTTGGGCTGTTTTTGCAGTTGTAACAAATGTTACATCAAAACCACGCACTGATTTAACATCATCATAAACAACTTGAGGGAAAATAATTTGTTCTTTAATACCTAAAGTGTAGTTACCTTGGTTATCAAACGAATTATTTGAAATTCCTTTAAAATCACGTACACGAGGTAAAGCAATATTAAATAAAGTTTCAATAAATGCTCACATACGATCGCCACGTAATGTAACTTTAGCACCAATACTTTGACCAGCACGTAATTTAAATGTTGCAATTGATTTTTTAGCTTTTGTTTCAACAGGTCTTTGTCCTGTAATCAAAGTTAATTCTGAAATAGCTGCTTCTAAATGTTTTTTATCAGCAACAGCATTTCCAATTCCTGCGTTAATAACTACTTTTTCAATTTTAGGAATTTGCATTACAGAAGAATAAGCAAATTCTTTTTGCAAATCTTTAGCAACTTTATTTTTATATAAATCTTTTAAGAATGCCATAACTTATCCTTCCTTTTTATTTTTTATTTACATCAAGTTCTGAACCACTTTTACGTGCTACACGAACTTTTTTGTTATCTTTAAGTAAATATTTAACTTTAGTTGCTTTACCTTTATCTTTTCCTTTAGGATCAACTAATGCTAATTTACATAAACGAATTGGAGCTTCTTTTTCAATAATTCCAGATTGTTCATGCGTTTGATCTTTCTTTTGATGACGTTTAATTTTATTAACGCCTTCAACAAGTGCTGTTTGTTCTTTAGGGTTAACTTGAATAACTACGCCAGATTTATTTTTGTTTTTACCTGAAATAACAACAACTGTATCGCCTTTTTTAATTCTGTTCATTATCTGCTAACCTCCTATAATACTTCAGGAGCAAGAGAAGCAATTTTTGTGTATCCACGATCACGAATTTCTCTTGCTACAGGACCAAAAATACGTGATCCTCTTGGTGTCTTATCCTCTTTAATAAGTACACATGCGTTTTCGTCAAATCTAATTAATAAACCAGACTCACGACGAATTGATTTTTTTGTTCTAACAATTACAGCTTTGGCCATTTGTCCTTTAGCAATTAAACCAGCAGGTGTTGCTTTTTTAACTGAGACAACTACAATATCGCCCACTGATGCATAACGTTTTTTAGAGCCACCAAGCACTTTGATTACTCCAACTTCTTTAGCACCTGTGTTATCTGCAACTTTTAGTCTTGTCATATGTTGAATCATAATCTTCTCCTTAGTTGCACTAACGTGCTCTTTCTAAAATTTTGCTAACTCTTCAATTTTTTGTTGCACTTAATGGACGAGTTTCAGTGATTTCAACAAGGTCACCAACTTTGGCATCATCATTATCATTGTGTGCGTGGTATTTTTTATGACTAATTACAAGTTTTCTATATATTGGGTGTTTGCTTTTAGTTTCAACACTAACAACAACAGTTTTTTGCATTTTATCTGAAACTACTAACCCTTCTAAAACTTTACGACGGCTTCTTTCCATTATTTATTAGCCTCCTTGTTGTTTAATTTTTCTGCTAATTCACGTTCATTTAAAATTGTTAATGCACGTGCAATTGTTTTACGAATTTCTTTGGCTGTATGAAGTTTTTCTGCTTCCCCATTCGCAGCTGCAAAACGAAGTTCTAATAATTTGGCTTTAAGCTCAATAACAATTTTTTCAAGTTCTAAGCTATCTTTTTTACGAAGATCTTGTGCAATACTACTCATTAGTTAGCTTCTCCCTTCTTAACAATTTTTACTTTAATTGGTAACTTATTTCCAGCAGCACGTAAAGCTTCACACATTAATTCTTCTGAAACATTAGCAATTTCAAACATTACTGTTCCAGCCTTTACAACTGCTACTCATTTTTCTGGACTACCTTTACCAGAACCCATACGTACTTCTAATGGTTTTTTAGTTAATGACATATGTGGGAAAATACGAATTCACATTTTACCAGTTTTTAATAAACGTTTTGAAATTGCAATCCGTGCTGATTCAATTTGACGCGCATCAATTCATGCACCTTCTAGTGCCATTAATCCAAATTCACCAAAATCAACTTGTTTATTTCCTTTAGCTTTACCTTCGTAGCTTACTTTATGAGGCTTACGAAATTTTGTTCTTTTTGGTTGTAACATAATTTTATAATCTCCTATTCGCTAACTGCTTGTTTTGGAGCAGGTTTAGTATTTTTTGATCTTGATGAACGATTAAATGATCCTTTTTTTGGTTTTGAACTACGACTAAATTCCTTATTTGCAGCATGCGCAACACTGTTTACTAATTTGTTACCAAATAATTCGCCACGATTAATTCAAACTTTAACACCAATAATTCCATAAGTTGTATGTGCTTCTTGCATTGAATAATCAATATCTGCACGTAATGTATGTAAGGTCATTACACCTTGTGTATATCCTTCTTCACGTGCCATTTCCACGCCACCTAAACGACCTGAAACATGTGTTTTAATACCTCTAGCACCAGCTTTTAGAACTTTTTTAATTACCATTTTTTGAGCAATTCTAAATGAAACTCTATTTTCAATTGCGTCGGCAATTTCACGTGCCATTAATGAAGCTTGTAATTCTGGAATTTGAACTTCAACTACTTTTAAAGATACTAATTGTTTACGACCAACGATTTTGTTAATTGCTAATTCAATTAATTTCTTTTCTGATGCTTCTGTTCCAATTAATAAACCACTTTGTACAGCATATACATAAACATTTACCCGTTGTTGGTCACGTTCAATTTCTACATGATCAATACCAGCATTTTTATATTTTGTTGATAAATATTTTCTAATCTTATCATCTTCAACTAATCATTTAGCCATTTGTAATTGATCAGTTGGCACTCAACGAGATAGTCATTGTTTGTTGATCCCAAATCTTAGTCCATTTGGATTTACTTTTTGACCCATTTGTAAAATCTCCCTTACCCTATTTTAAATATTGGAATCCACTTGGACCTTTAGTCATGTTATATTCGCTAAGATCTTTTGGATTACGGTATTTATATGCTTTTTTGCTTACAAGTGCATGTGCTTTATCTTTTCCTTCATAGTAAGCATCATATTCTTTTTTAGAAATTACAGATTGTTTGCTGTATTTTCTTCAAATTGATGAAATTGCACCAATTTCAACTGATTCTAAATCAAATTCACCAACAACTTTTTTAGTTGGGCTAGTTACATAAACTAGTACACGTAAAACTTTATTAATAGGTGTTGTTTTATAAAAGATTACATTTTTTTCTAAATCATCAAATAAAACTTGTGCATTCTTAGGTGATGTTGAAATCATAATTGTTTCAGTAGTTTTTTCTTCTTTTTGTGAAGCAGTTTTTTCAACAACTTTTAAAACTTGTTGTTCACGTTTTAAAAGTTCTGGATCAACGTTTTCGTCTTTTTCAACTGGTTTTATTTCTACTTTGCTTGGTTTAGCAACTTTTTTAGTTTCAACTTTAGCAATCGGTTCAACTGCTAATGGTTTTTTTGATTTTTTAGCTTTAATAGCTGCTAATTCTTTTTCACGTTCATTAACATCATCAGATAAAACAATTTCTAAATGTGTTGTACGTTTAAATAATTGATCAGCAGAACCCTTAGCACGTGGTAGTGTTCTTTTCATTGTTGGACCTTGATTAGCTACAATTTTGTAAATATATAGTTTACTTGGATCCATATCTTTAGAGTTATGTTGAACGTTTGAAATTGCTGAGTTAAGTAATTTCAAAACGATTGGAGCAAACTTCTTTGGTGTATTACTTAAAATTCGAATTGCTTCATGTACTGGCTTGTTTCGAACTAAATCAATTACAAGAGAAGCTTTACGGTGCGAAATATGAATATTTCTTTGAATAACTTTATTTGTCATATCTATAAATTACCTTTCTTTCTAACGTTTAGCTTCTGTATGGTTTTTGAAATTACGTGTTGGTGCAAATTCACCTAATTTGTGACCAATCATATCTTCTGTTACGTAAACTTTTAAGAAAGTTTTACCATTATGAACTTCAAATGTTAATCCAACAAAGTTTGGAAAAATTTGACTTCTTCTAGATCATGTTTTGATTGGTTTTTTAGAAACTGATGCATTAGCTGCTTCAACTTTTTTTAATAAACTAGGATCAGCGTAAGCACCTTTTTTTAAACTTCTAGACATCGCGATTAATCCTTTCTATATTATTTACGACGACGAACAATCATGCTCGTTGATGATTTCTTATTGTTGCGAGTTTTAACACCCATGTGACGTTTACCTCAAGGTGTACGAGGTGCATCCATACCAACTGGTGAACGACCTTCACCACCCCCATGTGGGTGATCATTAGGGTTCATTGCAGAACCACGAACTGTTGGACGAACCCCTAAATGACGTGATTTACCAGCTTTACCTAAATTTTCAAGAATGTGTTCATCATTTGAAACAGTTCCAATTGTAGCACGACATTCTTTTCTAAATTTACGAACTTCGCCTGAGTTTAATTTAACTAAAATATATTTTTTAGTTTCATCAAAACCAAGAATTTGTGCACTAGCACCAGCACTTCTTGTAATTTGACCACCAGCATTTGGTGATAGTTCAATATTGTGAACTAATGTACCTTCTGGAATAAATTCAAGTGGTAATGAATTACCTAATAAAATATCAATGTTTTCATTTCCTGAAATAATTTTATCGCCCACTTTAATTCCTTTAGGAGCAATAATATAACGTTTTTCTCCATCAGCGTAAACAACTAATGAAATAAATGATGTTCTGTTTGGATCGTATTCAATTGATTTAATAGTTCCATAAATATTATCTTTATTTCTTTTAAAGTCAATAATACGATATTTACGTTTATGACCACCACCATGGTGTCTAATTGTAATTTTCCCTTGATTGTTTCTACCTGCTTTTTTAGGTAAAGTAACAAGCAATGATTTTTCTGGTTTACTTGTTGTTAAAATCGATTTGTAATCAACAACAACAGTTTGTCTTTTACCACTACTATGGTTTTTAATACGTTTAACTGCCATAATTGCCTCTTTCTAATTTGCTTTTCGACTGTTATTTTTAATTTTGTTTTAAACTACTTTGCGTTTGCTGCTTCAACTTCTTCTTTACTTACAGCTACTTTAACACCTACTGGCATTACTACATAAGCAATTTTTTTTGCTTTTGTATATCCTGGTCTAGCTGTTGAAGTACGAATTTTTGCAGGACGACGATTTGTAGTACGAATTTTAAGTGGTTTTAAACCAAAAATCGCAATAAATGCTTCACGAATTTGGTATTTATTAGCATTTTTATCAACAATAAAAGTTAATGTTTCATGCTCGCTTTTATTACGAATAGAATAAGTTTTTTCTGTCGTATATGGATGTAAAATAACTCTTGTTAATTCCATGATTATTTACTTACCTTTCTTGCGTAATTTGTAAATGCTTCTTCAGCCACAACAACAACATTTGCATGCATTAAATCACGAACTGAAACTTGGTATCATAATTTTGTTGTAACTTTTTGTAAATTGTTTGTTGATTTTTTAATATTACTAAAATTGTCGACTATTACTAATAAAACTTTTTGATTTTCTAAGTTAGCATTATTAATGAAATTTACCATTTTTTTAGTTGAAGGTGTTTCTAAATCGCTATTTGCTACTAAACCTAGCATTTTTCCTTCATTCAATTTAATAGTTAATGCTGATTTGAAAGCTAATAGTCTTACTTTTGCATTAACTTTTAAATTGTAGTTACGATTTGGTTTTGGACCAAATACCACACCCCCACCTGTTCAATGTGGGTTACGTGTTGAACCTGTACGTGCTTTACCTGTGTGTTTTTGTCTTCATGGTTTTTTACCACCACCACGAACTTCACCCTTTGTTAGGGTTGAATGTGTACCTTGACGTTCAGCTGCATTTTCAGCAAGCACTGAGTCAAACATTGCTTGTTTGTGAGGTACTTCAACAAAAAGATCAGAGGTTACTTCAAGTTCTTTTGCGAAGTTACCATCAATAGATAATAATTTAATTTTTGCCATGACTAACTCCTTATTCGTTTGCTTCACTTGATGATGCTTTATTAGCTAAGTAGTTAACAACTTGTGGATCGCTTACGATATGGCCAACTTTTTTAGCTGATTTTAATAAAACAATTGAACCTTTTGGTCCTGGAATAGCACCTTTAATTAGGATTAAATTTTCATTTGCTTTAACATCTAATACTAGTAAGTTTTTAGTTGTTACTAATTCATGTCCATAATGACCAGGTAATTTAGTTCCTTTGAAAACACGTTGAGCTTGACTACCCCCACGTCCTTTAGCAATAGAACCAACATAACGATGTGGATAACCAGCACCATGGCCCATTGGACCCATACTAAAATTATGACGTTTAATTGAACCTGTAAATCCGTGTCCTTTAGTATATGCTTGTGCATTAACTAATTGTCCTGGTTCAAATTCACTAACTGTTAATTCAGCACCAACTTCAGCAACAACATCTCTAAATTCTTTAATGTGACGTTTTGGATCTGAATTTGCTTTTTTGAATTGACCTAATAATGGTTTGTTTAATGCTTTTTCTTTTACTGTTTCATAACCAATTTGAATTGCACTGTAGCCGTCTTTTTCATTAGTTTTAACAGCTAAAACCTTATTTGGTTCAACATAGATAACTGTGGCAGCTACAGCTTTACCTGTTTCTGTAAAAACTTGAGTCATGCCAACTTTTGTTCCTAATAATGATTTCATTTTTTTTACTTGCCTCCTAATGAATTAAATTTTGAAAGTAACTTCAACACCAAATGGAACTGATAAACGTTTTAAAGCTTCCATTGTTTTAGGTTGTGGATTTTCTAAAATAATTAAACGTTTGTGTGTTCTTCTTTCAAATTGTTCACGACTTGATTTATTAACATGTGGTGATCTTAAAATTGTAAAAATTTCCTTTTTAGTTGGTAGAGGAATTGGACCTCTTAATTTAGAACCTGTAGAGTTAGAAATGTTTACAATTGTTTTTACTGTATCATCTAATAATCTGTGGTCATAAGATTCTAATCTAATTCTTAATTCTTGATTCATAAAATGTTGTCTCCTTTTGCATCGATATATTATGCGAATCATAAGAATTGTGTATTAATGAGTTACCTGATACGTTTTATTGACAACGTTTCTTGGCGTATCAGCAATCTCAACAATAACAAAACCTTTAAAATTATACACGAAACAAAAGTAAAAAACAAGATAAAAATAAAAAATATACTATAACCAGCAATTGGCAAGTATATTTTTATCTAATATTATTTCATTACTTTATCAACTAATTGATTAAATGCTTCAGGGTTGTTAATTGCTAATTCAGATAGCATTTTTCTATTTAAACCTTGACCATCTTTAGTAACAACATTAGCTTTTACTAATGCATTCATAAATTGTGAATATGTATATCCTAATTCACGAACTGCAGCATTAATTCTTGAAATTCATAATTTTCTAAAATCACGCTTTTTATTACGACGATCACGATATGCATACTCTGCAGCACGAATTACTGTTTGACGAGCAATTCTATATGAGGTATTACGTGTTCCTCATGAACCTTCAGCTTTTTCTAATCATCTTTTACGACGTTGACGTGTAACACTTCCACCTTTTACTCTCATTGCTAATTTACTCCTTAAATACTATTTATAACTATTAAATTATTTATATTCTAACTATAAGCATTGAGTATATCTTTTTGTGTCAGATGTGCTCATAATTGCGTCTTTTCTTAAATGACGTTTAGCTTTTGTTGAACGACCTAGAGCTAAATGTGATCTATAAGCGTGTTTACGTTTTAATTTACCATTTTTTGTAATACTAAAACGTTTTGCAACAGCTCTTTTTGTTTTTTGTCTAATTTTAGCCATTATAGTTACCTTCCTTATTTTTTAATTGGTTCAATAATTGTTTCATATCGATAATTGCTAACTTTTTTATTAGCTTGAACAACTGTACCATAATCTTTAATTAAGTCAATGAATTTTTCATAAGCTTGTACAATAAATTCATCTTTTGTACACATACGTCCACGTGCTTCAATCACAAATTTCACGTTGTCTTTATCTTCTAATCAACGTTTAGCATTTTCTGCTCTTACTTTTAAATCATGTTCACCAATCATTGGTTTAATTTTGATTTCTTTTACCTTAATAATTGTTTGATTTTTTCTTGATTCTTTTTGACGACGTTTTTGTTCGTATTTGTACTTACCATAATCTAAAATTTTTGTTACAGGAATATTTCCTTTTTTAGCAATAACAACTAAATCTAAATTCTTTGAAGCGGCTAAATTAAGGGCATCAATACGATTCATTTCACCTAAATTATTACCATGGTCATCAATTACAACCATTGTTCGAAAACGAATTTGATCATTAATTAAAGGTTGATTTTTACGTGCATCATTATTTGATGACATATGGCGTTGATTGTTTGGTGTATTCATTAAACACTCCTAATTAAAAATTAAATGTATAAAGAAAATCATTTATATCTATAACATGAATAAATGATGTAATAATCGAAATATTAAGCATTAACCTATTCCCAAATAAACAGAAATCAGGTGAGTTTTAAACTACTTTCTTTATACAATATAGAATTATATATTATTTATGGGTAAAAAGCAACTTAATAAGTTAACTAATATAATTTAGGAATAATAATGTTTTTAGATTTATAAAATTCTTCTAATCCCTTATAAATTGACTCAGAAAATGATTTATCTTTTAGTACATCAACTGATTGTCCTAAAAATATCTTAACTAAATTTATTTGATTAGCATCTTTAGCATTTCAATAATTATAAAAAGCTCTTGAAGCTTGACGTTTATTTGGATCTTTTTCTAAATAAAATGAATTAGATAATTCATATGGATATAGTTCATTTTTTTGATCAACATAAACTAATCCACCTAACATTTTTTGTAATTGTATAAATGGTAAATCTTGATATTCGCTTTTTAATACTCAATCACCTTGTTGTGATTTAGTAGCAACTTCATCTAATTTTTTAGTTCCAATTTGTGATAAAAGTTTAAATAAAGGATCTTCTTGGATTATTTCAACAATTGTTTTATAATCATTTACTTCTTTTAAACCACTTAATTTAATTGCATCAATTAAATACTGATGTCCAGCTTTTGATAATTCAACTAACGCTGTATTATCAACATAAGTATTTTTATTAAAACTTGCTTCAGTGTGTGTTCCTAATTTATAAGCGTCTTTGTTAATATCATTAGTTAATTGTGCCCCTTCTGATGCTTTTAATAAAGCACCTTTAAAAGCTAAATCTAAACGTTTAACAATAGAAAAAATAATTGTTTTACCGTTAGCTAATGATTTATTATTTTCACTAATACGTGCTTTATTTGTATTATCATCTAATTCTTGTTCTGTATCAACACCAACAACAACGATTGGTTTACTTGATTCAGCAGCAGCTGTTGTTGCATAATCGATTTGAGGTCCAGCCACTGGTAAAATTAAATCAACATTATTAGAAACTAATTGATTAACAATTGCTTTTGCTTTATCTGATGTTGGCGAAAAATCTCCTGATTGATAATTCGTAGCAAAGACTTGTTCTACATTAATTCATTCTTTTTCTTCATTATTTTCTGTTTGTTTAACTTTTTTGTTTTTTAGTTTTTCATTTGCTCATTGAATACCAAATTTAAAACCTTGAATAAATCTTGTAGTTGATGGTAAATGAATACCAACAAAACCGCCCCATGTTAATTTATTATCTTTACCAAAAACATTTTGATTACTATTTAAATAATAAGCTGCAGCAATTCCAGCTAAAAAAGCAGCTTGATCTGCACGATATGTGATTGATGCAATTCGATCTTTACCTGTTTGTAAATCATAAATTTCATCAATTAATACTGCTGAAACTTGTTGATTATCTTTTGGTGAATTAAAGTATTTTGAAATTGGATTTTTATGTCTAAATGAAATTAATCCTAACATACGTGCACCATCTTGAATTTTAGACGCATAGATTGCTTGATATGCATCATCCCCATCACTTGATGGGCGACCATAAAAATTGTTTAAAGTTAAGTTTCTAGAATCAGATTTTGTTTTATTTTGACTACATGCAACCAATGCTATAGGAACCAAAGCTACACCCATTATTCCAATAACTGATGCTAAAATAATTTTTTTATTTAATTTAATTTTTCTCATGATTTTCTCCAAACAAATTCTCATTTACACAAATTAAAAGGTTAATTATATATTTATATATACAAAAAAGACGTAAAAAAGAAACAAAGTAATAATTATTACCTTTTTAGTATAATAATTGGAACATTAAAAAGGTAATAAAAAATGACTTCAATATAAAGTCATTTTTTTCTTTTATATTAAACTAGATTCGTTTTGTTGCTTATAAAATTTATTTAATTGTACATAAATAATAATTGATCCGATTATAGGTATTATTCCTAAGCACCCTAAAATGCCAAAAACAAATGGCAAGACAAAGTTTTCTTGTCTTTTTAAATTTAAACCAACATTGACTGTTGTTGTTACAAATAAAGCAAAACTAGCAATTTCAATTAAAGCAAAAATTACTAAAAAAGCCACTCCAGCATTATACATAGCTCAAGCATTTACAACATTTGTTGTAGCTATATCTTGCGTTAAATATGGTGATCCATAATATGAGTAAACCATATAATAAATGCCAAGATGCACTAAAATAGCGCCAATTATTATAAAAACAACTACTCCTATACTTAAAATTAAATTTGCTAATGCAATATCTAAATCTTTTTTATTAACAATTTTGTTCTGATTCACGATTTATCCTTAATTTTGTCCTAATGTTTTTTTCATTCCATCAATTGAAACTTTTAATGAAGCTGTTAATGCTTTATACGCTTCATCAGATAATTTTTCACTCTTATTGATTTGTTTATTAATTTCTTCTTTCAAAGTACTAAAAGCTTCTTTAAGTGCTGGCATTTTTGAAGCTAATTTGTCTAATCAACTTAAATATTCTTTATAAGTTTTTTCAAATTCTTTTTGTGCTTCACTCTTAGTAGAACATGAAGCAACAACAATTGGAATAGCAACTAATAAACTAGTTGGTAGTGCAATTGTTAAAATTTTTTGTCATTTTTTCATATTTCATTTCCTAAACTATATTTTTTGTGTTTTATACAATAAATTATGTATTTAATTTATTTTGTTGAAAAACTTAAGCTAGCTAAAAGCTCTTCTGTAATTTTGATTGATTTATCATCATATCTAATATCTTCAATTACAAAATCACTATTAGCATCTAATCCATTTAATGTGAATTGATAGCTTCCAATAGGTTTTGATTTTTCATTGAAATTAAATTCTTTAACTGTATTTGTTGCTTTATTTTTAACAAAGAAGCTAAATTTTATTGCTTTGTCTTTTTTATTAATTAATTTGTTAGCACCAACAATAATTGAATTACCATTTAATTTTAAACTTAATGTTATATTGGCACTGCTACTATTTTGTTTTGTAATACTAATAGATTTTAATTCTTGATTACCAAAAACTTTTTTAGTGATATCGATGATTTTTTCCACATTTTTAATATGTTTTTCACCAACAGTAATTGTGAATGATTTAAATAAGGAATTGTTTGCAGTGATTTCTATTTTTTCACTAATTACAGGTACTCTTGGTAATTTAATTTGAATTTCTGAATAATCTTCTTTAACGTAAGGTCTTTGATTTTCTAATATTGCATTTTTAAGATTTGCAACATTTGAGATTTTTGAAGTAAAAGTTACAGATTTTAAAATTTCTTTTGTTGGTTCATTTAAATTTTGAGGGTCTTTAACTTTTAGAACAACATAATATGTTTTATCATCAAGTTTCAAAATTTTTTCTTGTACTTCATATTCAATTTTAGGTTTAGTTTTTGTAGTAAATACTTGATTATTACTTAAAATAACTTCTTTATTTGTTTGATATAAAATTTTGTTAATTTTATATGTTTGACCGTCTTGTAAATTATTTAGTTTTAAAACTAATTTTTGATTTTCAATTGTTGCAGATAAATTACTTGCTTCAATAACTTCATTAGTGTTTTGAGTATTTTTTAAAATAACTTTAAAATTATCTAATGTTTTAAAACTATCATTATTATCAAAAATTAATTTAGCTTCATTAAAACCAGTTTCAACACTTGATAAACTTAATTTATATTCTTCTATAGTTGATGGTTTATCTGGTTTTGGATTAGATTTTTTTCCGTCTTTACAACTTGTTAATGCCAATGTTGATAATCCGCCAATTAATAAAATTGGCGTTGTCATTAATAATCATTTTTTATGATTCTTCTTCATATTTTAACCTTCTTTTATACATTTAATAAATAAGAATTTTAATAATTTGATTTGTTATCTTTATTTTATATAAGAGTATACATTTAGATTATATATATATATAATATGTCAAGTGTAAAATAAAAAAATAAGTCAAAACACTTTTTAAAAAAGTATTAAGACTCATTTTTGTTTTTTAATGTTCTTCTTCTGGTGCAAATCTTGTTGCGATTTCAGCTGCTAAATATTGCATTTCATTCGCAACTAATTCTCTTTGTGCCTTATCTTTTGTTGTTCTTACAATTACTTGTTTTTGTTGTAATAATTGCACATGACGTTCAAATTTAGCAAATTCTTTTTGATCTTTGTATAAATGTCAATTATTAATTACATCTAAAACATTTTGATCAACGTTTGTTAAGTTTGAATGTTCTGATAAATTAATACCTCGTTTTTTATTTCAAGCATAATTAATTAGATAAATAAATAATCCAAGTAATAATGTGCATATAAACATTGGTAATGCAATTGAAATAAATACATAATAAGCTTCTTGTCCAGGATATAGTTCTGGAGCAGCTGGTAAGTAAGTTGCAACAACTGCAAATGTATTAACGCCCATTGCTAATAAAGCTAAACTAATTTGTAATCATGATCATTTAACTCTAAATTCACGACGTAATCAATTAGCTTTAAAGACATATCTTAGATATCCAATTAAGAAAATGTTATATCCAATATAATACATTGAGATTTGTGAAACAACGTTTGAAATGAATGAAATATTAGTAATTGATGCTGGTGTTCCCGATGCTACGGCATTAATGCCAATATAATCTTTGATTTTATCATAAATTGCTTTTACTTCACTGTTAGCATGTTTATTAAAATCAAAATGTAAAACATTAGCTTGTGCTCCAGGAGCATGACTATGTTCGTAAATTGAACGAGCCATTAGTTTAATTTCATCTAAATTTTGATTAGACAATAAAGCCATTGGTTCTTTTTGATCTTGGTTAATACGAATTTCATTAGCTAAAATATCACTAATTGTTTGACCTTTGTATTGCATTAATGAATTATAACCAAACATGTAAATTGCAAAAATGCACATTAGTAATACAATTCATAAAATTGCTACTGTTAACGCTGACCCATTCTTTAAACGATATGTAACCCATGCTGGGAAGTGGGCTTTACGCATTGCATAATTTAATCCAGTTGAAAGGTTTGACATTCAAACAGAAACTTCAACAAATACACCTATTCCAATGAAGAAAGTAATAGCTTGTAATCCTTGCATAGCCATTTTATTTGTTTCTATATTCATAACAGAAATAAATAAAGATGGGTTTTTTAAAGGATCGTTAACTTCTGATGTTAGTGTTAATAAATCAGAACGTGCTAAGTCACTACCTGTATTAATTGGATGACCATACACAAATTGATACATAACTTGGTTAGCGGCAGCTGTGCCATATTGAGTTTCAATTACTGAGTATGCATGTGCTAGTGTTTTATAATGAGCAACTGTGCCATCTGGTAGTTTAACATCAATTCCACCAACAGATAAGTTGAACATAATTGTCTGTACTAATCCACCATTGAATGATAATGTTGAAGTTGGTACAGTCATTGAAATCATAATTGATCCAATAATAATGCAAACTAAAGCAAAAATTACAACAATCATCATGGCTTGTGGATATTTCTTAGAAGGATTATCCATTCGATTAGCAGCAATAGCTGAAACTTCTACACCATGTAAGCTAAAACTAAATGCCATGAAAGATGCCATAACAACTGATGACATGAAAGTTTTATTTAATTCATAAGAAGTACCAGTTACACTTGGGTCTAAATGTGGTTCTTTTTGGAAGTGTTTTCCAATAAAGTATAATGGATCTGGCATTGTTGCCACTGTATAAATTTGGAATCCTAAAATAATAAAGAATGGTAGTAAAATACCAATGATTAAAGCAACTTGGCCAAATTGGTGAGTTCGTTTTTGGCCTAATAAACTCATAAAGATTAGACCTGCGACAACAACAATACCAATTCCATATAATGCCCCATTGCTAAAGTTTTTTGAAAAGTTGACTGATGTAGCTTGATTTAAAGTTGTTCCCAACTTAAGTGTATTAAAATAATTATATCCATCAGCACCTGCGAAAGCAAATGAAAAGGATGAAAGGACGAATAAGATCATAGCCACAAATCCAAATGTGACTTGGAATTGTAATCAGGTTACAATAAACCCATTTCGTGGACCTAATAAATTTCGTACCCAGGTAAATATTCCCCCCTCGGCCCAACCTTTAATTGTTGCCATTTCAGCGGCTGCAATCCCAATTGGAATGAATCAACACATAGCTGTAATAAAAGCAAAGATAATTGCAATTCAACCAGCTGTTGAAAGTGATGGGAATGTATAAAAAGCAATAATTGCTGAAGCTGTTAGTAAAACAAAACCCATTAAAGAAACTTTTTTGTTTTTTTCTAGTTTCTTTTTGATTTTTGCTTCTTTTATTTTATTATTTATCAATTTTTTATCACCTCTTATTCTAAAAATATTTGAAACTAATTTTATTTTTGATAAATCGCTTACGACCAATCAAAAATTTGCTCATAAATTCATTACTATATATAAAACCTAATTTCTAATTTAGATTATATAATAAATGCTAGTATTTCCTATGCTTTTTAGCATAAAAATGTCGATTTTTAACATTTTTATTTTTTATTTTTACAAAAATTTATTCTGCATCATTTTGGTACATAATTTTGTTATAAATTCGACGATTTGTTTGTTCATTTAAATTTAAATAACTAAACGTTGTTTGAATGTTTGCATGTCCCATTTGTAACATTACAGTTTTGGGATTTGCCCCTTTAATTAACATAAAAGTAGCAAAGCTACGACGTAATGAATGGGGAGTAAAATTCTTGCCAATAATTTTTTTAATATATAAACGTAATGTTTTAGTTGTTTCAAATCCTTTTAGATTCTCATAAAACTTTTGTAATTGTTCAAAAGTTTGTTTTACAAAGAAAATTTGTCGTTTTTTATTTCCTTTACCTAACACATACAAACGATTATTAATAGATTCTAGAAAAAATAATTCATGAGCTCTAATTCCTGTTTCAAACAAGAATCTAATAATGGTTTTATATTTTTTAATTTCTAATGAATCATCATCTAAGATATCAGTTTTTTTATATAAATTATTTTTACTAATTACATCAAAATATTTCATTTCAATTTGTGGTAATTTTAATAATTTTAAATTTTGTAAATATCTTTTTAGTTTTTTAAATTCAAAAAAACTTAATAAAACGTTTTTGTGTAAATGAATTGTTCGTGGTTTAAAGTTACTATTAATAATTTTATTTCGTATTTTAATTCACGAATCTAAAACAGGTTCATAATGTTTTAATACTGATTCATAAGTACGAATTGTGTTAAGACTTAAATTTCTTTTTTTAGTATATCTAATAAAATCTTTCATATTATCTACTTTTCCTTTTTATGATTTCTTATTTTTTTATCTTCTAAAGTATGAAAAAAATAAACAAGCATCTTTGAAGCGATCAACTTGTTTATTTTTTATTTTTTTAATAATAAAGATTTTTAATAGTTATTTCATAACGAAATTTTTGGCCGCTGGTTCTTTATTTAAGCCAGGCATACGATAAATAGTTGTTGTAATAGCAATGATTAATTTTGCAGCATAATTAATTTCAAAGCGTTCAATATGAATTTTAAAGTTCTTAGGATTATTTAATAATTTTGCATCATCAGAAATTGAATATGGTGTTTTAGCAATGCATAAATAAAAATCTAAATGTTCATAACGATTCAATTGTTCTTTAGCTTCATAGCTTAATTCATATCCATCAGCACCATAAACATTTTCACATATTTTTTTTAATTTATATGATAAATGATCTTTAATATCATAAATTAATTTTAATTCATGATCATTAATTTGATTAGTTAAAGTAATTGTTTTTTTAGTTATTTCTTCACTTTTTTTAGAACCATAGCTATAAGCATAAGAACGTGCATGTTCAATTTGATGTTCATCTAATAGTTTTTCTAAAGTTAATAAGTCTTCTTCGCTATCAGTATTAGTATTGACATTAATATAAACAATAAAAGAAACATTATATTTTTTAATGTGATTAATATGACATAAAACATTAGCAAAGCCTTGTTTAACGTAGTCTTTTTCGTTTGTTTGAGCATGATATGCGATTGATTTTAGACTAATAACTAAACCAATTAAATCTGGTTTTAAATTCAAACTAGCCATCTTGATATTCATAAATTTTTCAAGTCCTAAATCGCTACCAAAACCACATTCAGTAACAACATAATCACCTAAAGCTAAAGCATTTTTGGTTGCAATAATACTATTACAACCATGAGCAATATTAGCAAATGGGCCACCATGAACAAAAACAGGGTTATCCTCATTAGTTCGTACTAAATTAGCATATAAAGCATCTTCTAAAATTGCCATAATAGCTTTTGTTAATTCTAAATCACAAATTCTTATAGGTTGGTTTACTATATTATATGCAACAATTGTTTGCGCAAGTTTATCTTCAAAATCTTTATGATCATGTGCTAAACAAAATAATGCCATTAAATCACTCGCTGCTGTAATGTTGAAACCACTTTTTGTTTGTTGATGGTTAATATCATATTCAATATTACGTAAAGAACGATCATTAACATCTAGACATCTTTTAATTAAAATTTTTTGAGAATCGATTTGCATTGAAGACCTATTATGTATTTCATTTTCTATTACTGCAACGATTAAATTATTAGCTGCAGTAATCGCATGGAAATCACCACTAAAATGTAAATTAATTTTATCAAAGGGTTTTAAAAAAGATAATCCTCCTCCAGAACCCGTACCTTTCATACCAAAAACTGGTCCCATTGATGGCTCTCGTAAGGCTCCAATTGCTTTGTATCCATGTTTATTTAAAGCATCAACTAAACCAATAGCAGTTGTTGTTTTACCTTCACCAGCTGGATTAGGATTCATTGAGGTAATAACAATTAATTTTGAATCCTTTTTATTTAGTTTTTTAATTGATTTAATTTTAGCAATCTCATTGCCATAAAGTTCATAATCATCAATTAACAACCCTTTTTTATTAATCATTTCTTCAATAATTTTTGCCATATATAAACCTTCTTTTATTTTTTGATAGTGTATTTTCTTTTTAAAATAGTATACATCAAATAAAAAATTAAACCTAAAATAACAGTTAACACTAAAAATATGACTAGACCACGACGATTTGCTTGTTTTTTTAATGAAATTATTTCTTCACTAGTAATGATTTCATTAACATAATAACTATGAATACGATTAATACGATGATGAGCATTAAAAACCAACCATAAATGAATAATAAATGTTAATCCTAAAATAGCACCTAATCCAATCATTGCCCATAATGGATTTTTGTTAAAACTAATTTTTTGTGAAAGATGATTATTAATAACAACAGGAATTTTTATAATCAAATCACCAAAACGACTTCCTATAGCTGCATTATAATATAAATTCATAAAATAACTGACAACAAAAACACCAAATATCATAAAAAGACTAGTAAGATAAATATAAGCTGTGATTCAGTTTACATTAATATGTACTACTATTAATTTACGATATATACCTACAATAAAATTTGTTGCTTTTGTACAATCAAAATGGTTTTTTTGATAATCCATTTCTTTTTTAAAATTTATAAAATTACTACATGATAAAGCTAAAAAGAAAATCATCAAGACACAATAAATACTAATTGGTAAAAAAATCCATGGATTAAAATGATTAATAATAACTTCATAAATCAAATAAAATATTAATCCTACGCCAATTGCTAATAAAATAGTTCAAATGATAATTTTGTTACGAACTGCATGAATTTCTTTTAAATAAAAATCAGGTGTATTTTTTTGATTAACTTTTTCTTCTAGTTGACTAAAAGTTAAATTATTTTCACTTCCGTATGTTTTTGGATCGTCTTCATTATGATAAATAAATTCTGAAATATTTTGAGGAATAAATTGTTTAGTTTTTAAAGCAAAACCATTGGATTTTTGACTTATAGATAAATGATTATCATCTGTTAAGTCCTGATCTATTATTTCTTTTTTTAATTGATAATCAACAGTGTTTTTTTTATCGATTTCAATTTCTTTTAATCTTAAATTTTCATAATTTTTAGTAGGAACAATTGAAATTAATTGATCACTATCATCAAAATTTAGAGTTGGTTTTAACTTTAAAATATTGTCTTGTTTTTTAATTAAGATATCTTGTGAATCATTATTAGGAGAAAAACCAGCATTATATTCTTTCGTTTCTAATAAGCGAGTGTATTCAATATCATCACAAATTTTATTGTTATTACTTTTAATTCTATTTTTATCATTATTTTCATTAAAAACTTGCGTATTTATTCGTGGCATTCATTGATCCCCCATTTAAGATAAATATTACTATTTCATCAATTATAAATTATAAATAAAAAAGAAGTTAGTTTTAATTAACTTCTTAATTATGAAATACTGATATTTTCTATTTTGTACACTCACGAGATTTAGATTGTCATGTACAATCATTCTCATTCTCTATAAAAACAGGGTTTTTCTTGGCAGCTAAGTTGCTTTTTTTACAACCACTAGCTTTACATTCTCAAGTTTGATCAAAACTTTCATATTCAAACATTTCACTTTCTGAAGGAATAATAAAATCAAACTTGTATGCATCTTCACCATGATTGATGTAGTCTATATGGCCTAATTGTTGACGCACTGATAAACGAGGTTTAGTAATATAAACTATTAAACATGTAAAGGCAAATGCCCATGTTGCTGCTAATAATACAGCACCTAATTGTAGTCCAAATAAAATACCATAAATTGGATTTCCATTATTATCTAATCCGACGGCGTCATATTTAATTCCATGTGCAACCGTGTTAGTAGCAAATGCTCCAATTAATAAAGAACCTATAACACCACCCATTCCATGAACTGGTAGAACTTCTAAAGAATCATCAAAACGGAAGTAATCTAATAATCTAGAAACTGAATAACATACTAAAACACCCATAATGGCAATAGGAACAGAGGCTCAAACTGGAACAAAACCTGCTGCGGGAGTAATTGTTGCTAATCCAGCGACTGCACCAGTCACTAAACCTACTGTTGTTGGTTTGTGGTTTTTGTTAACAATAGTTTCAAGTGTAACTCAACCTATCATACCTATTGCTAAGGCAATAATTGTATTTAATCATGATGATATAGCTTGTGAAATGTGATTTGTATTTTCTTTAACAAAACCTGCACCACCAACATTAAATCCAAATCAACCAAAAAATAGTAATGCTGCACCTAATACAACCATTGGTAAATTATTAGCTCGCTTTTTATCTGTAATTAATGTTGTACGTTTTCCTAACATAAATGAAGCAGCCAAAGCTCCAAAACCAGCAGTTGTATGAATTACAATACCACCAGCAAAGTCAATTACTCCAGCAGCAGCTAAGAATCCTTGACCTCAAATTCAATGTGCAAAAGGAATATAAATTAAATATTGTCATAGTACTAAGAATACTAGATAATTTTTAAATTTCATTCGGTCTGCAAATGCACCAACCATTAATGGTGGTGTAATAATTGCAAAAGCTAATTGATAAGCAAAGAATAAAATTAATGGTACGCCGTTAGTAAAATTAGCAAAAACTAAAACTGCTCCTCCACTTCAACCATCAGCAAATAGTAGATTTCTAAATGCAAAATAAGTTGAAATATCACCAATTATTCCTTTACCAACAGAAGGTCCAAAAGCAAGTGAGAAACCTCCAAAAATTCAAATCAATGTTGTAATTCCAAGTGAAGCTACACATTGATTAATAATTGTTAAAGCTGATTTACGTCTTACTAAACCACCATAAAATAATGCTAACCCTGGTGTCATTAGTAAAACAAAAGCAATTGCTAAAGCTACTAGTAGTGTATTAGTAGGATTAAAACCTATTGCATTAAGTGTTGATTGACTTGCATCTGCGGGCACTCATGTTAATTCATGAGTTGAGAAATAAATATCTTTATTTAGGCCTCAAGTTGACACAATTTTCACCTCGTGTTTTCTTTTTCGTTTTCGATAAATAAATTTTGTACATAAATTTATAAATACAAAATATATCTATAGTATTATATTACGTTAATTAATAAATTCTTTATTTTTTTATTATTTATTAATTAAAAAAACTAAAGTATTTTGCTTTAGTCTTTTTTTAAAAATTAATGGTCGTTTGCTACTTTATGATTTTGCTGGTTTTTATTATTAAGCAATGAATACTTAACACCTGTGCTTGAGTGTCATGTACTATCTGCTAATTCATATTGCTCCATTTCTTTTTCAGATGGAATTTCAATATCGAATGAATATGCATCTTCGCCATGATTAATATAGTCTATATGACCTAATTGTTGGCGTGCTGATAATCTTGGACGAGTAATATATACTAATAAAATAGTAAATACAAATCCATAAACCGCAGCTAATAAAACCGCTCCTAATTGGACACCAAATAAAACACCAGTTGTTGTTGGACCAATTGCTTCATACATAATTGATGGGTTTACAGATTTAGATGCAAACGCACCAATTAATAATGAACCAGTAACACCACCCATTCCATGTACGCCTCATACTTCTAGAGTATCATCAATTTTGTGGAAATGATGTAAAGTTTTAGCACTAGTATAACAAACTAGCACAGCAGCTACACCAATAGGAACAGATGCTCAAATTGGAACATAACCTGCTGCAGGAGTAATTGTTGCTAATCCAGCAATTGCACCTGTTACTAAACCAACACCTGTGGCTTTATGATTTTTATTAAAGATTGTTTCAAGAACTGCTCAACCAACCATTCCAATAGCTAAAGCAATAATTGTATTAATCCAAGCACTTGTAGCAGCTGATCAAGTAGCAGCATTCCCACCAGCTACAAATCCTGATCCACCAACATTAAATCCAAATCAACCAAAGAATAACAATGTTGCTCCAATAATAGTCATTGGAATATTGTGTGGACGACTTTTATCATTCTTAAGTAAAACACGTTTACCTAATACTAATGAAGCAGCTAATGCTCCAAAACCAGCAGTTGTATGAATTACAATACCACCAGCAAAGTCAATTACTCCAGCAGCAGCTAAGAATCCTTGACCTCAAATTCAATGTGCAAAAGGAATGTAAATTAAATATTGTCATAGTACTAAGAATACTAGATAATTTTTAAATTTCATTCGATCTGCAAATGCACCAACCATTAATGGTGGTGTAATAATTGCAAAAGCTAATTGATAAGCAAAGAATAAAATTAATGGTACACCATTAGTAAAGTTTGCAAAAACAATTCCTACATCTCCACTTCAACCATCAGCAAATAATAGATTTCTAAATGCAAAATAAGTTGAAATATCACCAATTATTCCTTTACCAACAGAAGGTCCAAAAGCAAGTGAGAACCCTCCAAAAATTCAAATCAGTGTTGTGATTCCAAGTGAAGCTACACATTGATTAATAATCGTTAATGTAGATTTTCGACGTGTTAGCCCACCATAAAATAATGCTAAACCTGGAGTCATCAATAAGACAAAGGCAATTGCTAATGCAACTAATAAAGTGCTAGTCGGATTAAAATTAGTTAATGCTAATGTATTAGCATCAGCTGTTGCTGGCACTCATGTTAACTCATGCTTTGAAAAGTAAATATTTGGATCTACTCCTCAAGATGTAGTTATAATTGTTGACACAATTCTCACCTCTTATTTATTTTTTTTATACTTTAAAACAAAATATAATTTTTAAGTACATATTACTATTATAGTACTAAAAAAATAAAAAGCAAGCACAAGCGAATTATAAAAAGTATTTTTGCAACTAAAGAATTAGAATTATTTATTTACATTTTCTATTTTGAGCACGTGTTTTTATTTTCAACACATCATTTGGTAATTCTTTTATTATTTTTAAATAGTCATCATATCGTCAATTAACAATTAATTTTTCATCAACAGCTTTTTTAATAGCACATTCTGGTTCTTCAATATGTAAACAATTAGAAAATTTACAAAAACGTGCATAATTTGCAAAATCATTAAAGGCATTAGCCATTTCATTCTTGGTTAAATTAACCTCTAAATTTCCAAAACCCGGTGTATCAACTAAAAAGCCATTAGCAAAACTAATTAGTTTGGTACTTGTTGTTGTGTGTTTTCCTCGATTCAGAAAATGTGATATTTCCTGTGTACGTTGTTGAATGCTTGGATCTAATTTATTAATTAATGTTGATTTACCTACGCCTGAATTTCCTGCCAAACAAAGTGTCTTGCTTTTAATCTTTTCTTTTAATAATAAAATATCATTTTCTTTTGTTAATATAAAAACCGCATAGTTATTTTTTTTATAATCTAAAATTAACTTATTAATTTTTTGTTTTAAATGATTATTGGCTAAATCATATTTACTCAAACCTATTAAAACATTTTTAATATTGCGAGCTTCATAAAAAGCTAAATATTTGTTTAAACTCAATGTATTTAAATCTGGTTGTACAATAGAGACTACGATTAAAACAATATCAATATTTGCAACTTTGGGACGAATTAATTGATTATAACGTTCATAAATTTTTGTGATTAAATAAGTATTCCCAATTAATTCAACTTCAACATCATCCCCAACCATTGGTTTTAATTCATGACTGTTATGTTTAAAAATCCCTTTGGGAATTGCTTTTATCTGAATTTTTAAATCATAAAGATGTACATAAAAATTATTAGCAACAACCGAAATAATCTTTGCCCGCACAATTAGATTGCCCCACTTTTTTTAAAAAATAAAATAATAATTAATAAAATGATTAGAATAACCGATAAACAACTAATAAGAATAATAAATCATTTTTGTAAATATCATTTACGATCTTTTTCTTTAATTTCATATAAGCCTGCTAGATTTTTACGATAAATTCGTAACTTATTTAATGGTTTAATGTATTCTTGATTAAGATTTGACTCTGGATCCATAACATTACTTAAATCATCAATTAAAGTGTAGATATCTTTGTATAACAACTTATTATCTTCTTTTTTTCAAGCAAAACAACGCATTAAAATATTATCAATACCTTTTGGTATTGTTTTATTAATTAACGATAGTGGTTCAACATCATATGTTATTCATTTTTCTAAATAATCACGATCATCTGTAATTGTATGTTCACTAAAAGGGTTAGTTCCAGTAATTAATTCATATAACATAACTCCTAAAGAATGAATATCATAACTAACACCTAATGCGTCATTAATTAAACTTAAAGCTCTTTTATTTAAACCCTTATTAGCTTGTTCTTTAACTTTTTGCCCAAGACTTAATAATTTTGGTGAAGCGTAAATAATTGTACATTTAATTTCTTGATCTTTTGTTAAATCTTCACGATTGTAAAAAACCGATGCTGCACCATAATCAATAATTTTAATTTTTGTTAAGGTTTTATTAATCATGCAATTTTCTGGCTTTAAATCACGATGAATAATTTGTGGATTACAACTATGAAAAAAACTTACACCTAAGGCAACCTGACGAATAATTTCAACAGCCTTAACATAAGTAATTGTTTTAAATTCATCAAGATATTCACGCAATGTCAAACCTTCAATGTATTCAAAAACAATATAAATCCTTTTATCATTATTTTCTAACGAACCGGTTTCAAAATATTCATAATATTCCATAATATAGTTTTTTTTATCAGAATCAGAATTTTTAATTAAATTTGAAATTCTTAATTCATCATAAACAACCATTTGTTGTTTCTCATCATTAATATCTAAAACTTTAATAACAATAAACTTAGTTTCATCTGATAAAAAACAACATAAATAAACTTTACTAAAACCACCATCTGCTAGGTGTTTAACAACTTTATATTTATAATTAAGAATTGAGTTTGTTTTTATTTCTTCACGCATAAAATTTAAAAAATTAAGAAAGCTAATGATAAATTATCATTGCTACCGTTGATAATAGCTTGTCTTATTAATTGTTGTAAAAGTTCATTTTTTGTCTCTTGGGTGCTTTTTATTAATTTTTCATATAATGTATTTAATTCAAAATAGTTATGGAATCCATCTGATGTTAAAACAATAATTTCATTTGGGTTTAATTTATTTACGAATAAGTCATAACTCAAAAAAATATTACTTGTGCGACCAACAAATTGTGTTAATGAAAATAAATTCTTCTCATGTTTTTTATAAACCTCAGGGTTGGCTTTACGTTCTTGTAAAACTTGTAATAAATTATGATCGCGACTTATTTGTAAGAATTGTTCATTGGATAATAAATAAGCACGCGAATCACCAATATTTAGAGTGTATGAAAAATCATTAACAACAATAGAAGCAACAACTGTAGTTCCCATATTATAAACGTCTTTATCTAGTAAAACAGTGCGATCAATTTCAACCTGTGCTAGTTTAATAACTTTAATATACCATTTTTGAATCTGTTGTTTATCATACTCACTAAAATCTGTAGTTAAAAAATTATCTTTAATTGTTTCTAATGTAATGTGGCTAGCCGCAGCACCACCTTTATACCCGCCTAATCCATCACAAACAATTAATAAAGTTTGTTGATATTGATTTTGAATTACCAATGCACAATCATCATTATGTTTGCGTTGTGAGCCAATATCACTAATAAACCCAAAATTCATTTTTTACTCCTTAATTTTCAATTGTTTAAAACTAATATCTAAATCGTTACTTTTTCGTATACGCTTACTAATTCGTATTTTATTAACAGATAAAATATTAAAAAAATCATCTAAATTAGCACTAGTTCGATAATTATTTAAATTATAAGGAATTAAAAGCACATAACATAAGATGTTTTTTAATAATTCTACTAATTCATTAGCATAATTTAAATCATCATTAATTTTATCAATTAATAAATATTCTAATACAACGCGATTATTTGTAATCTTTGTATATGCCTTAATTTGCTCAATTAATTTTAAAAAAGATCAATTTTGATTTAGTTTCTTCAACATTATTTTTTGATAAATTAATGAGTTTGAAGCATTCATACTAATAATCAATTCTATTTGATTTTGTAATTGTCCTCATTTAATCAAATCAACATTTATACCACTTGTTCAAACTGCAATTTTCCTTTTTCCAATATTTAAACCATTTTCATTATTAATAATATTAATGAAGTTTGATACAACGTCCATATTTAATAAGCTATCCTGTAATCCTTTTATAATAACATTAGTAATGTTGCCTAATTTACGTTGATCTAGAAATTGTTGTATTAATAAAATTTGTAATACTAATTCATTAGTTGATAAATTAATAATTTCTTTTTTAAAATTAATTCCAAACGGATTTATTTTTATTAAAAATCCGTTTTTAAATTTAATAATCATGCAATTAATAATCAGATTTTTATCTGTTAAAAATTCAAACTTAATTGAACCATCAGAATTTGATTGATAACTGTTAATTTTTAATAGATTATTACTAAAATTTTCTTTTAGAATTATTACATTATTTTTAGCAATATTAGAAAATTTATCAAAATTAAAAATTCGTTTCTGATAAATCCAAGAATAAATTTGTTTGCTAATAAAAGATTTTAAGCCTAATTTAAATAATTCTTCTTTAAGTTCATTTAATGATAATGAATAAATTTCTTTTAGATTATGATTGTTTAATTTCATTTAGAATAATCTCCGCCAACTGGTTTGCTGCATGATCTGGATTATCATTAATAATATTATATTGATAATAATCTTTTATTGTTATTTCATGAGTAGCTTTTTGAATTCTTTTTTTAATTGTTTCTAATGTTTCTGTATTCCTTTTTAATAAGCGTTTTTTTAATTCATCAATACTTGGTGGTAATAAAAAAATAGAAATTAAATCATCATTTTTATATAAATCTAAAACTTTAAGTGCACCTACAACCTCAATTTCTAAAATAACATTTTTTCCTTCATCTCTTAATTTTTCAACAATAGTTCGTGGTGTTCCATATTTATTTCCAACAAACTCAGCTCACTCAATTAATTCATTATTTAGTATGGCCTTTTTAAATTCTTCATCATTAACAAAATAATAATCAACACCATTAACTTCACCTTCACGTTTTTTTCGGGTTGTCATTGAAATACTATATGCTAAATTTAAATCTTTACGATTTATGATTTTAGATAGAATTGTATGTTTTCCTACACCACTAGGTCCACTAAAAACAATTAATTTTCCGCGTTTCATAAATTAAAACTCCTCTTTTTTATTTTTATGGTTATTATTAATACCAATGTTTTTAAAATTTAGTAATTTTACATCTCTAATTTTGAATAAATTTACAACTTAATAAATAACACATGAGTTAATTTATATTATATAAAGAAAACATCCAAATAAACAAGGTTAACCTATTTAATTATGATATAATATTTAAGCTGTGTATGATTTAAATTAACGTATGCAGCTTCAACCACTCAAAAAAGGTTTAAAAATAAATGTTAATTTATACCTTATTGGTGAGTCTAAGTTGATAAGATATCAATTCTAAGGAGAAATTTGTAATGTTTGCTATTTTTCAAACAGGCGGAAAACAATACAAAGTTCAACAAGGCGAAAAAATCTATGTTGAAAAACTAGATTTAGAAGTTGGTAGTAAAATTTCATTTGATCAAGTTATTATGGTAGAAGGTAGTGTTGGTACTCCTTTTGTAAAAAATGCTGTAGTTAATGCTACTGTTATAAAACAAGGAAAACAAAAGAAAATCAACATTATTAAATTTAAATCTAAAAAACACCATTTAAAACGTCAAGGTCATCGACAACCATATACACAATTAGTTATTGATTCAATTAGTGTTAAGTAGTTTTTATGATTAAAATAAATCATTATCCAAACGCTTTATTAGTAAAAGGTCATGCAAATTTTGATGAGCATGGCAAAGATATAGTATGCGCTGGAGTGAGTGCTATTATTATGGGTGCACTTAATTGATTTGATCAACAAAAAACAACAATTAAGGTTGAGCAAGGATTTATATTAATAATTATTGATAATAACAATCAATTGTATCGTCAATATCTAGAATTAATTATAATTCAATTAAAAGCAATCTATTTTAAATATCAATCTTATATTGAATTACACGAATATAAACAACAATATAAAAGAGGTTTATAATTACAATGAATAAATTATACTGATTAACTGACTTACAACTTTTTGCTTCTAAAAAAGGGGTAGGTTCATCTAAAAATGGTCGTGACTCAAATCCTAAATATTTAGGCGCAAAACTTGGTGATGGGCAATCTACTAAAGCAGGTCAAATTATTTATCGTCAAAGGGGCAATAAAATCTACCCTGGATTAAATGTTGGACAAGGTAAAGACCACACTTTATTTGCAAAAACTGCTGGTGTGGTAAAATACACTAAATTTATGGGAGATAAAACAAAAGTTAGTGTTCTTCCAAAAGAAGATAATAATTAAAGAGGAATTATTCCTCTTTTTTTATTTTAAACATATTACTTTAAGTAATTTGATTAATGTTTAACAATTATCTTTTAAAAAATCTAAATATGAATATTAATATATATACATATATATTCTTCTTAAGATTTTAATCTTCTTATTTTATATTTGTGTTTATAAATTATTTCTAACGTCATTTTTCAATAAAAATTGATGATTTGTATATAATTAAGATTTGAATTTGTTATATTTATAATGTATGATTAACATTAAAAAATTTATTAATTAAAAACGGTTATTAAAACTTAATGTCAGTCATATAAATTTGTTTATTAACTAGAGTTTTAATTAAGTGGACTTTAAATAAAATAACCAGTTAATAATAAAAAAATGTACAAGCAATGGTTTGTACATTTTTTTATTTTTAACATTTTCGTTCACTAGGCTAATAAATTATGCTTTTTAAGTGTTTTTAGTGTTTTAGCTGAAACTAATACACGTTGGCTCCCGTTTTCAGTCTTAATAGTTGCTTTTTGTAAATTAACATTTCAACGACGCTTTGAATGATTCATAGCATGTGAACGTGTATTCCCACTTAAAGGACCTTTGCCTGTTAATTGATCTCTTTTTGCCATCTTTTTAAACACCTCCACGTGTTAAAATTATTTAAATATTTTAATTTCACTAATTTTAATATTATAAAATATTTATAGTAATATAATAAATTTTTTTATGAATATCAACTGAAAAAGTATGTATAAAGAGATTTTAATAAGAAATAGTGATTTAAATTATATTGTTAGTGATCCTTTTGAAAACATGATTGGAGGTTTTCCTATTATTTTATTTGAAAATGATAATTATATATATTATTTATCTGGTCAAAATGCTTATTTACCTTCAAAAATGCGTCGTTGAAAAAACTGAGATGAAATTTTGATCAATAAAAATGACTTATTAAAAGAAAATTTTATTTATAAGGATATTTATATTAATTTATTTCAAATCTATGTTACTAAAAAAAATAATTTAAGCAGATATTTAAGTAACCAGTTTAATTTTACGTTAACAAAAAACGATTTTAAAGACGAATTGTGAAATAAATTAATATATAATACAATAAAAAAATTGATTAATAATTCCAATCCACAAATTTTACTATTTGAAATTTCAAGAAATAAAAAGCAAAAATATCATAGTTTATTACTTTTTGCTGATAAAAATGTATTGAAAAAATTTTATTTAAATTCTGCAACAAAAGATAATTATGAAGATATTATTGATTTGGCCTTTAATAAATTTGAAAATTTACAAAATTTATTAAAAACAACAAAAGTTAAAAACCATTTAAAAAATTTGAAAAAGTATTAAGATTGTATTTATCTTATTTGTCTAAAAACATAAATAATAATTTTATAATATAATATAATTTTGAAACTATAAATATGAGAGCGTAAATGTGGTGGGAGTAGGGCATGTTAAAAAAAACTAAATTTGATACTCAACTTGTTAAAAGTTTAATTACATCCTCTATTTTAAAAGTTCCTGGGATTTTTAGTGTTGACATAAATGACAAATTATCAGATTTTAATCGTAATTATTTTGTAATTAAAATTGATTTACATGAAGATGTTGTTAATGTTTTAAGTGTAGCAAATGAAGCGCGTAATTTAGTATATTATGAATTGTCAAAACAATTAAATGATGATAATGTGGTTATTAACATCATTATAAACTGTTAAAATGGAAGTGTGATATGAAAACAATTAAGTTAGATCTTTTTCAAAAAATGTTATATGCAGGTGCAAAAATATTAGAAATTGAATGTGAATATATTAACGATTTAAATGTTTTTCCTGTTCCAGATGGTGATACTGGTTCAAACATGAAAACCACAACAGCTGGTGCAATTATTGCTATTAAAGAGATAGAAATATCCAATTTTTCCCAACTTGCTAAAATTTTTAGTCGTGGTCTATTAATGAACGCCAGAGGTAACTCTGGTGTTATTTTAAGTCAAATATTTCGTGGATTTATGGATGCTTTAAATAACGATGCTTATGAAGTTAGTATTGAACAATTAAAACAAGCTTTTAATGGTGCAAAAGAAAAAGCTTATGCAAGCGTCTCTACACCTATTGAAGGAACAATTTTAACAGTCATTCGCGTTATTAATGAGCGAATAAGTGAACGTCAAAATTTTGATAGTGTCGAAGATTTATTTGCATTTGTTACAAAAGAAGCGCAAATCATTCTAGAAAGAACACCAGAAATGTTACCTTCGCTAAAAGAAGCTAATGTTGTTGATTCTGGTGGATATGGTTTAGTAAGTTTTCTAAAAGGAATGTATCAAAGCTTAACAAATACATTTGATGAAAAATTAATTATTAAAAACGAAGAAGAAAATATTAAATCAAAGACTAAACCGCTTGATCTTGAAATTATACATAAAGAAAAAGAAGAAGGTTTTGGTTATTGTAGTGAAATTATTTTATCTTTACGAAAAAAAATTATCCCTAATTCACCAAGTAAAAGTTTTTTTGAATTTGAAAAATATAAAAGTGAATTAGCAAAAATCGGTGATAGTATTGTAGCAATCCAAGATGAAGATCTAGTTAAAGTTCATTTGCATACCCTAACTCCTTATAAATTCTTACAATTATCACAAAAATATGGTGAATTTGATAAGATTAAAATTGAAAACATGACAAACCAATTTTATGAAAGTTTAGAACGAAAAGGGTTGACTACAAAAAAAGAAAATAAATTGACTTTAAGTATGGAGCAAAAAATTATTGCAACAGTTCCATCTGAAAAGTTTAAAAAAATGTTTATTGAAGAACATGGTATTGATAATGTTATTGTTACTGAAACAGAAGGAGCTCCATCAATTCAACGTTTTGTCGATGAAATTAACTCAACAAACTCTTCACAAATTTTTATTGTCACTGATGATTCAAATATTGTATTAGCTGCTGAACAAGCTTCAAAAATCGTTGCTGAAAACAACATTTTGGTTTCTGTAATTGGAGCTTATAATGTTTTTGAAACGCTTTTAGCGATTTCTCATTTTGAACAGCAAAATGATTATCGCACAAATTTTAAAACAATGAATAAAATAGTTAAAAAAGCACGTAGTGGGATGATTACTACTTCAATTAAAAATGTTAAATATCCACATATTACTGTTAATAAAGATGATTATATAGGCATTATTAATAAAAAAATTATCGCTTCTGAAAAAAATGCTTATGATGTTTTATTAAAAACAATTGAAATGTTAATTGAAAGTTTAAAAAAACCAGAAATTTGTTATATATATTATGGCATTAATGCTTCACAAACAGATGTACATCAAATTGAAAAATACATTTCAGAAAAATATGGAATTATTTGTGATTTAAGATATACTGGTCAAAGTTTATATCATTACTACATAGGAATTCAATAATATTATGGAAAAAATTAAAATTTTATTAGATACAATGGGTTATGAAAATGATTTAGAACATGTAATTAAAGCAGCTAAAGATTTTTATTATCAACATGAAGATGATTTAGAAATTATCTTAGTAGGAAATGAACAATTAATTAAGCCATTATTAGATAATGATTGACGTTTATTCCCTATTGTACATACTGAAGTAAGCATTGAACAAAATGATACTATCTTATCAGCTCGTAAAAAACAAAATTCATCAATGCATTTAGCTTTAAGATATTTAAAAGATAAGCAAGCTGATGGAATGTTAACAGCTGGAAATTCTGCTGTTTTTGTTTATAATGCTTATGCTACAATCGGATTATTAGAACATATTAAGAAACCTGCTTTTATGCCTTTTGTTCCAACAATTGATGGTGGTGTTACAAATTTATTAGATGTGGGTGCATCAATTGATGTTGATGGTACAGATCTATTTAATTTTGCAATCATGGCTAATACAATTGCTAAAATGCGTACTCCAAATCCTCGTGTTGGCGTTTTAAATATTGGTACTGAAGATCATAAAGGTTTACCATATCATCAAGAAGCTAATGAATTATTAAAAACATCAAACTTAAATTATGTGGGTTTTGTAGAACCAAAAACAATTTTAGAACGAGAAGTTGATGTTTTAGTTGCTGATGGTTTTAGTGGAAACATTGCTTTAAAAACAATGGAAGGTGTTGGTAAAACAATTTCTAATTTTTTAAAAAATGAATATAAAAAACCAAAAAATCTTTTTGCTGCTTTATTGTCAAAACCAATTTTTAAAAAAATAAAAAAAGCATTTGATTATAAAGAACATGCTGGAGCTTTTGTTTTAGGATTAGATGGCATTTTAGTTAAAACCCATGGTAGTGCTGATTACCAACAATTTATGAGTGCTTTAAAAATTTTGTATGAAACAATTAAAGCAGATGTTTTAAATGAAATTAAAAAAGATTTAAATAATTATTATGGACAATAAAAAATTTTTAGATTTTTTAAAACAAAATAGAATTGAACCAAAAAATTTATCTATTTATCTTGAAGCATTAACACATAAATCTTATGCAAACGAGCATAAACTTACTAAAAACTATCAACGTTTAGAATTTTTAGGTGATGCTTGTGTTGAATGGGTAATTAGCAATTTTATTTTTAACTACAAAATTAAAGATAATGAAAAAATGCGTTCTTTAGATGAAGGTGAAATGACTCGAGCGCGTTCTAATATGGTACGAAGCGAGATTTTATCATATGCTGCTAAAGATTTAGGGTTAACTGATTTTTTAATGATTGGTGTTGGTTTAGAACAAGACCAATCAGCTCGTATGGAAAAAATCTATGAAGATATTTTTGAAGCATTTATAGGTGCTGTAGCTCAAGATCAAGGTATTAAAAAAGTTTCATTAATTCTTGAAAAAACTTTAATTAAATATTTTAGAGAAGGCCAAATTAATTATCAAAAAGATTATAAAACGATTTTTCAAGAACAAGCCCAACGTATTAATAAAAAACCTATTATGTATAAATTAGTACGTAATGAAGGCGATAAAAAAGAAGTCCATTTAGTTTGAAATGATTTAATTTATGGAATTGGAATAGCATCAACGCGAAAAGAAGCAGAAATTTTAGCAGCTAAAAATGCAATTTTAAAACTTGATGATTATACAAAAAAAGCATAATTATGTTAAAATTATTAGATATTGATTTCTTGCATAGTTTGATTTAAAATCTTCGAAATTAAACTAAGTTAGGAATAGATTAAAAAAATATACAATTCAAGGAGATTATCATGGCAGTAAGCAAACAACAAAAACATGATTTAACAGTTAAATTTGGTGGTTCTGCATCAAATACAGGAAAAACAGAAGTTCAAGTAGCAATCTTAAGTGCTGAAATTGATTCATTAACAACACATATGATTGAAAATAAAAAAGATAAAGCTTCAAAGCGTGGTTTATACAAAAAAGTAGCTCAACGTAAAAAATTATTATCATACTTACAACGTGTTGATATTGAACGTTATCGTGCTTTAATTAAAGAATTAAACTTAAGAGGATAAAAAAACATTAGTTAAATTTAATTAACTAATGTTTTTTTAATTATAGGAAATAAATATCATTAACCTAAAAGATATATGAAAAGCTGTTTTTTTATTAATAGATTTTATAATTTTAATGTGTGTTTCTTTTAGATTTCGTCCTTTTCTTAAAAGAAGTGCAAATTAATTATTAAACTAAATGCCTATTATCTTTATAATTTGTCGCTTATTATAAGGAATGTTCTCCAAATTCATTATTATTTCATCTTCGCATGAGTTTATTAATTAATTAAAACAAAAACACTTGTTTAATATACAACAAGTGTTTTTTTATTTAATTAGTAAATTAAAAAATAAATAAAATTATTTATGTAACATAGCCTCATTGATAATTTTTAAATTAAGTTTATGAATTTGCTTTTTATTAACTAGACGCATTTTTAATAATCTAAAAATTAATGAGTTTAATACAACAGCAATATCACTAGTTGTCATAATTAAAACAGAAATAAATGGTGGAATAATACCAATTAATGCTAGTGGAATAAAAATTAGATTATAACCAAACGCTCATAGTAAACTACTTACAATAAACATCTTAGTAATTTTTGTTAGTTTAATTACTTTGTAAATATTTAAAATATCTGGTTTGATTAAACTAATATCAGCAACTGCATTAGTTGCTTTATTAGTTTCACCAATTGATATAGAAACATTAGCTTGTTTTAAAGCTAATAAATCATTAATACCATCGCCAACATACATTACAGTTTTTTGATCATTTTGAATTTTTTTAATAATGTTAGCTTTTTCTTCTGGTTTAACTTGAGCATAAAAATGTTTAATTCCTAATTCCTTTGCGATTTTTTGAGCAACATTAAAACTATCACCAGTAATCATATAAGTTTCAATATTATTTTCATGCAATACCTTAATTGTTTCATAAGCATCAGCACGAATTTCATCTTCAAAAACAAGAATTGATTGCACCTTTTTGTTAATACTAAAAATAATATTTGAAGCTAATAAATTAGTAGAAGTAGAAGATTTTTGATTAATCAATGAAAAATCAAATTGATTTTCATTTGCATAATGTTCTGATGTTAATTCATAAATATTACCATCAACATCTTTAGCAATAATCCCCACTCCTGCAACTTCTTTAATATCAATTAATTTTGTACTCATGACGATATTATTAATAATGGCATAAGTTACGAATGATTTTGCTAGTGGATGTAAAGAAATTAATTCCATTTGATAAATTAAATCTAAAAATTGATCATTTCCTATAACTTGTTTTAATTGTAATTTTCCATAAGTTAATGTCCCTGTTTTATCAAAAGCAATTGCATTAACTTTTTTAATCTTTTCAAAAATATTACTATTGTTAAATGTAATCCCACTTTTAGCACCATGCCCTGCACCAATTAAAACTGCTAAAGGCACAGCAATACCAAATGCACATGGACATGATATAACTAGAACAGAAACTGCTACATATGCTGCTTTTTCATATAAATTAGAATCATTATTTGATCCATGAAGATTTAAAAAAGCAATTGGTAACTCTTGAATACTTGGTACAAAAGCTTGTAGTAAAAAAGCTAGAATGGCCATTAATAAGATAAATGGAGTGAATCATAACGAAATCTTATCAGCAACAACTTGAATTTTTGGCTTGCTACTAGCAATTTGATTAACACCATTAATAATTTTTTTAATCGTTGAATCATTATAAAGTTTAGTAATTTTAAAAATAAAATTATCTCCAACATTAATTGATCCAGCAATAATATCATTATTAATTGTTTTTAGAATTGGACGAGCTTCACCAGTTAATGATGATTCATCAACTTCAGCAATTTGTGAATATAATACGCCATCTAATGGAACAATTTCACCTTTTTTAACTAGTGCGTATTCGTTAATTTTAGCTTGAAAAACAACTTTTTCACTAACTTGTTTTGTGTTCAAATCATAACTATTATATTTTTTGACTTGTAAATTATTTAAAGATTGTAGATCTTGATTTGCTTTTAGTTTAATACGATCACTAACTAATTGACCAACACTCATTGTCGCTATAATCATGCAAGCTGTTTCAAAAAAACCATGTAGTTTAGGTTCATAAATAATTGTGTTAGAAATTAAACTATAAGTACTATATATGAAAGCAACACATGTTGAAATAACAACAAGTAAAGACATTCCAGGTTTTTTTCATTTAAAAACTTCAAAAAAAGCACCCTTAAAAAAACTAAAACCTAAACCAAAGACAATGAAAATACTTAAAACAAAAACAATTCATCCATATATAAAATAACCATCAATGCTTAAAATTAAATTACTTTTAAACATAAATAGCATTTCAAAAACCATTAAAGGAATACTAATTAGAAGTGCTATTGATGTTTTCATTAAAGAAATTAAATATCTTCTTTTATTAATAGGTAGTAATTGAAATTTTTTAATTAAATTATTCTTTAACATTTTTTAGTATATGTATTTTTCTAAAATATTTGTTTTGAAACCATTTTTATTGATTTCTTTAATTACATCTTGATCAGCAATTTTATTTTCTTCAAATTCAAAAGCAAATTCTGAATTTAAAATATTTACACTAATATTTTGTGCGCCAATCTTTTCAAGTACATTATATAAATTTGAAGAACATGACCCGCAATGTAAGTCACTAGATGTTAATAGCATATAAGTCATAAATAAGACCTCCAAGTAAAATAATTGGTACACTAATATTAAAACACAAAAATATAAATTTGTTCCATTTTTTTCACTTTTTATGTAAAATTTTTGGAAGACTTATTTTTTATATAAATAAAAAAACTAGGTAAATGCCTAGTTTTTGCTAATGATTTATTAACGACTGGTAATGTGCACATTACCAAAGTTCTGCACAATATTTATGCCATGTTCAATATTTCGTTCAATATGACGATACATTAAAATTAATTTAATATAATTTGAGTTTTCATCTAATAGTTTTGATTGATCATGTAAGGACTGAATTAAATTTTTATTGATTTTCATAATTGATTTAGAACGTAAATCTTTAATATGTTCAATTGTATTGACACTAAATTCATTTTTATCAAAAAAATCATATAAATCAGAAAGAATTTGATTAGAAATCTCAAAGCTAGTTAAAAAAAAATTTTTTTGTTCTTCATCAAATTCAGTTTCTAAAAAGTATTCACAAATAATTTCAGAGTGATCAGAAATTCTTTCTAAATCTTTAATAGAATTGATTCCTGCAATAAAAAAACGTAAATAACTAGCACGTGGTTGGTCACGTTGAATAATTCACATAATTTCTTCTAACATATCAGCATATGTTGTATTAACTTTTTTTTCTAAAAAACAAGCTTTTTCATATGTTTCTTTATATGATGATTGATCGTTTTTTAATAAATCACAAATTAGTTTTTGATTATCAATTACCATTTGAAAAAAGTTTTTAAAATCATTTAGTAAATCACATTGTGATTCTTTCATTAAAAAATAATTAGTAGCCATAAATATATCACCAGTTCTTTTTATTAACCAATCTTTCCAGAAATATAATCTTTTGTTCTTTTATTTTTAGGATGGATAAAAATAGTTTTTGTTTCTCCCGCTTCTTCAATTCACCCTTGATAAAAAAACACAGTTTCATCACTTATTCGCTGGGCTTGTGCCATTGAGTGAGTCACAATAATAATAGAATATTTCTTTTTTAATTCTAATATTAATTCTTCAATTTTAGCTGTTGCAATTGGATCTAAAGCACTTGTTGGTTCATCCATTAACAACACTTCTGGCTCTAAAGCAATTGCTCGCGCAATACATAAACGTTGTTGCTGACCACCTGATAAAGCGTTCCCAGCTTTATCTAAATCATCTTTAACTTCATCTCATAAAGCTGCACTTTTTAATGATTTTTCAACAATTTTTTCTAAAATTTTACGATCATTAATTCCATTATTTCGTGGACCATATGCAACATTATCAAAAATACTCATTTCAAATGGTGTTGGTTTTTGAAAAACCATCCCGATTCGTGTTCTTAATTCTAATGATGATATTTTTTTTGAACGTGTATTAGTTCCTAAAAAATAAATTTCACCTTCGTGACTAGTCCCTTCTATTAAATCGTTTAATTGATTTAAATTTCTTAAAAAAGTTGATTTACCACAACCACTTGGGCCAATAAATGCTGTTACTTTATTTCTTTTAATATCCAAATTTAAATCATGTAGAACATGTTTTGTTCGATTCATATATCAAAAATTAAAGTTTCGAATTTCAAAAACATTTGCGTGATTAAAATCTTCTTTTAGCAAATCAACTTGCGTTTTTTTCTTATTTAAAAATTGCTTTAATTCAGCTTTTTTTTGTTTTGGAAGTTTTAGTCAAGCAAAAGCTCTTTGGTATTTATTTAATTTTTGAAATTCATCATAAATTGCTTTAGCATCCAATTCATTTAAATTAGTTTTTTGATTTAAAGGAACAATTGTTTGAATTTCTTTATTGTCTTTTTGATCATTCATATTTTTATCCCCTAACTTTTAATAACTCTTTTTGAATTTGTTCATCATCAACATAACGAATTTCATATAACTTTAAACCAATTTTAGCAACTTTATTTACTGATTCATTATATCCCATGATTTTGGCTTGATTATGTGTTAAATAAAGATGTTTTTGATAAATTTGTGGTTTGAAAACTTTAATATCACGCATGTAAGGTGCGCGAAGATAATTTTTTAAAATTTTAAACCATTTTTTAATATCATGTTTAATAAATTCAAAATAAGGAACAATAACGTGAGCAATGATGATTAATGCAATAACTAATCATAAAGTAATAAAAGCAGATTCGTACATTACATTATGAGCAATATTTGTATTATTTTCATATAATTGAGCATAAATACGTGTTGTTAATGTTTGTCCAGGATTTAAAATTGTTATGCTTTGAGCTGATGATAAACCTGCTGTTAAATACAATGGTGCAGTTTCAGCTACAATTCGACCAATTGTTAATACAATCGCAGAAATAATTCCTTTTTTTGCTTGTGGTAAAACTAATTTAACAATTGTTTCTCACTTTGAACATCCCAATCCATAAGCATTTTCACGAATGCTCATTGGTACTGCTTTTAAAGATTGTTGAATTGAACGTGTAAATGTTGGAAGGACTACTAAAGTAATTGTTAAAGCACCCGCTAATAGTGATTTACCAATATTACCCATTGAAGTAAAACCAAAAATTTCAATAAAAATAACTAAACCAAACATCCCAAAAATAATCGAAGGCGTTGATCCAAATGAATCAATAAAAAATAATAAAACTTTTTTTGGTCATTTATTTTTAGCATATTCATTAATATAAATTGCGACAAACAATGAAAATGGTAAACCAATTAAAATTGCAACAATAATAATTATTAAAGTATTAATTGTTGCTTGTCCAGTTGTGTTTTTTGTATAAGCTACTACTGTTGATGTTGGTAAATTAATTGCTTTAATTCCATTAACTAAAATATCTAGTGAAATTCAAGCTAAAAAAGCAAAAGCAACCATAAACGCTAATGATTCAAAAAATAATTTGTGAACAGTATATAAACGTTTTAGTTTTCGATTTTGAATACGATCTGTAATATAGTTATTTAAGTTATCCAAATTGTTTACATGTAATTTATATTGGGAACGATGCGTAATTTTCTCATATAAAATTTTTAATTGATCTGGTATAAAACAAACTATATAAGCTAAACTTTTTTCTAATTTAATAAATCAAGCATATGTTTTCTTTGAACGTTTTCTTGTTAAATAAGTTACAAGGCCATTTAAAATCATAATCGCTACAAATAAAACGATCCCATAAATATATAGTAATCCTCTTAAACCTTCGCCACCATTTTCAGCGAACATGTTAGCTGAAATTACAGCACCTAATGGACGAAGAGCAGAGTGCATAATTTGTAAAAAACCAGTACCAAAAATGTTTTGGTAACCTTGATCACTTAAAATCATACTAATGGCCATTGTTTCGCCGATCGCACGACCTAAGGCAATAATAATGGCAATAATGATACCACCACGAGCTTCTTTTTTAAAAATCTTATAAATTGAACGTGTATAAGAACTTCCCATTCCTATCCCATTTTCAATTAAACGCATATCAATACCATCATATGTGCTTAGCGTTAAAGAAATAATTGTCGGAATAATAATAAACGCTAACATAAACATTGACGTAGTAATGTTTTGAACAGTATCAATGTGGAAAACAAACGAAATTGCAGGACCTAAAGACTGAATAGCAAATAATCCAAAAATAACTGAAGGAATTCCAGCTAATGCTAAAATCGTGACTCTAAAAAATTTTTGTAATCTTTTGTTTTTAATTCTAAACTTAATAAAAGTAGCTGTTTTAATACCAATTGGAGCAGCAATTATAATTGCACCAAACGAAACAAGCAAAGTAATTGCTAATGGAAATCAAACTGATGAACCAGCAGCATAATCAGCTAAGTTAAAATTCTTGCTTCAAAAAATGTTTTTTAATCCATAAGCTTCAAAGCCAGGTATTGAACGAATAATAATAAAAACAACTAAACCAATAAAACAAATAGAAAATAATCAAGCAAAACTAATAACTAACGTTTTAGCTAGTTTATCTTTTATTTTTTTGTTAAAAATGATTTTTTTAGTCTGTTGCGAATTAAGATTTAAAATACTCATTATGCTGATTTTGCTCCTAATATATTAATTCTATTTGATTCTATTAATTCAAAATCACTTACAAAAAAATTGTTTTTTGCGCTTTGATTGTTAATGGCTTCTTGAAAAGATTGTTCATCTTTATAAGGTTTTAAGAACATTGAATTTTTTTGTTCAAAAGTAAGGGGAATAAACCCTGATTCTTGAATGATTTTTTCAACTTTTTTATTGGTTAATAATCATCAAACGAATTCTTTAGTATTTTTAATTCCTTGCATATTTAAACTAAAAACTAAATTAAAAGGTCGATATCATCCATAAGTTTTAGCAACATTAACAATTTCTTGTTTGTTTTCATTAATTTCAAAAGGTAAATAATTTTTTAAATCTTTATTTTGTAAATAAGCAATCTTATATCCTTTAGCAATAATTTGATCATAATTTTTAAGAATAAAACCTGTTGATAAATAAGTTATTGATCCATCTACATTATCTTTAGAAATATTTGTTCAAGCTTGAGTATTCGATTCTTCAGTACTTCTTATTAATTTGCCATAAGTACCAGCTTCAATAACCTTATAAATATCATGATATTTTTTTTTAAATTCTTTTTTAGAAATTAATGGATTAGAAGTCAAAAAAGCCTCGGCTGTCCCTGAGGCATTTGCTCCACCTGTGCGTGAATAAGCTAAAATTGGTGTTTTTGGTAATTGGGGATCAAGATCATAATAACTTACTTGTTCATTCCCAGTAAAAGCTTTAAACAATTTAATAATATTGTCTTTTGTTAAAACTAATTCATGATTAGTTTTATTGAGTTTATAAATAATGCCTATACCATCTCAAGCAATTGTATAAGTTTTCAATTGATTTTGTTTTCACTTATCATAAACTACATGACTTTTTTCAACACTTTGTTGCTCAAAATCAATATTTGGACTACGTGAACTTACGCCTATATTAGTTCTTTGTTCAACAGCTTCAATTATTCCTAACGAAGAACCTCCAGCATTAGAAATAATATCTTGTTTATGATGTTTTTCAGATAAGGCCTGAATTAAGGGTAATACTGATGAACTACCATTTGCATAAACAATTGGAATTTGTTTATAATCAATTGATATTGCAGCAATAACACCAACAACACCAACCACTCCTAAGCTAGCGCATAAGATTTTATATCTTAACCTCATTAATTCCTCAACTACTATTAAATGTTAGTCTTATAAATTATATAAAAAAAGCTTAATTATTTTAATAATTACAAAGAATTAAAGATTTTATTGTTTTTCTACAATGATTCTTGCAAAAATTGGTTTTGAATCATTAACTTTATGGTAGTTGAATATATCATAAGATGCTAAACTAGCTAAAGTTAAAAAAGATTCATCAAAATTTAATTGTTCAACTGCTTTTTTACTTCCTTCAATTAAAATTGGTGATAATAAAGTAGTAGTTACTTGAATAACTTTTACTAAATGAACTAAAAGTGAATCTAATTCTTGCTCTTGTTGATTTTTAGCTAATTCTCAAGGTTTATTATCTTCAATATATTTATTTGCTTCAACTAAAATTTCTTGAATGCAATTAAGTGCTTTATCAATACTATATTTATTAATGTTTTCTTGAACTAATTCAATATTTTTATTAATTAAATTTTCTAATTTTTCATCATTTTTTAAAACACAACCAACATATTTTGGAACTATATTATTTCGATATTTTTTTAACATTCCCAATGTTCTTGAAATCATGTTACCATAACTATTTGCTAATTGAGTATTATAAGTTTCAATCAAATTATCTTCACTAAAAATAGCATCTCTAAATAAACTTAAATCAGCCATTAAATAATAACGAAGTGCATCCACACCATAAGTATCAATTAAAACATTAGGATCAATTACATTGCCTAATGATTTAGACATTTTGCCTTCTTTTGTAATGATTCATCCATGTGATAAAATTGTTGATGGAAAACGAATTTGTAAATCATTTAAAAAGATTGGTCAATAAATGAAGTGAAAACGCGCAATTTCCTTTGATAATAAATGAACAATTTCAGTTTCATTATCACATCAATATTTTTGAAATAATTCCTCATTATTTGATAAATAACCAGTTGCAGTTAAATAATTCATTAATGCATCTAATCAAACATAAATTACATGTTTTGGATTTTCAGCAATAGGAATCCCTCAATCAAAAGTAGTACGTGAAATACTTAAATCTTCTAAATTATTTAAGAAGTTATTAACCATTTCATTTGCACGTTCATTTGGAATAATGAAATTCGGATGATTTTGATAGTAAGTTTTTAAAAATGGTGCTTGATCACTCATTTTATAAAAATATGATTCTTCAGACTTAGTTTCTAATTTATGACCCATGCGACATAACATTATATTATCTTGACTTTTAATAATTTCAGCAGGATTATAATTTTCTTCGCATGATACACAATAATAACCTGTCCATTGGCCTAAATAAATTTTTCCTTGTTTATATAAATAACTAAAAACTTTTTGTACAGACTCTTCATGATCCATTTGCGTTGTACGAATAAATTTTGAAAAACTAATATTTAATCGTTTTCATAAATTTAAAAAAATAATTGAATTACGATCGACCAAAGCTTTGGGAGAAATGTTTTCTTCAAAAGCTTTTTGTTGGATTTTTTGACCATGCTCATCCATTCCTGTTAAAAAGAAAACATCATATCCAAACAAGCGTTTATAACGTGCTAAAACATCAGCAATGATCGTTGTATAAGCATGACCAATATGAGGATTTCCAGAAGAATAGTAAATGGGAGTAGAGATAAAAAATTTCTTTTGTTTTAACATATTTTTCTCCTTAAAATTTTTAATTATTATTTTTAAATTTTTGTTGATTTTGGTTTAATTTAAACTCTTGTTCGTTGTTTTGGGGAACATAATATTTTTTGTTTGTTAATTCACGTGGTAAATATTGTTGATTAACATAGTTATTTTTAAAATCGTGTGGATATTTATAACCATGAACACCCAATTTTATTGCTGATGCATAATGAGCATCACGGACATGATTTGGAATTGAATATTGTTTGCCATTAGCAGCATCTTCAAATGCTTTTTGATAGGCAAGATATACAGAGTTTGATTTATTTGATAAACAAATTTGTAAAACTAAATTAGCTAAAATTACTTGATTTTCTGGATATCCTAAAAATCGACAAGAATTAATTCCTTGATCAACACGTAAACATAGATTAGGATTAGCTAAACCTATATCTTCATGAACACAAACACTAACTCTTCGATAAATCGCTTCAAAATCACCTATGATTATTAAACGTGCTAAATAATATAAAGCTGCATCACAATCACTTCCACGCATTGATTTATGAAAAGCTGATTTTAAGTCATGAATTTCATCGCCATCGCTACTACCTAATGCGTATGATTCTTGCATAATACGTTTTAGTAATACATAATCAACTTTATGTTCTTTATATAATAAATAAATAATTTCTAATGTATTGATAACAGCCCTTAAATCTCCATTAGTTTTATTAATTAATAAATTAAAAGCTTGTGGTTCAAAAACTTCTTCAACATTAATCAAAGTTTTAATTCGTTCACTAATTTGTGAACTCGTTAATGGTTTTAATGATAATAGTTGACAACGACTTCTTAAAGCAGGGTTAATAACAAAAAACGGATTTTCAGTTGTTGTCGCAAATAATTTAATTTTATTTGCTTCAATAATTGGTAATAAAATATCTTGTTTATCTTTATTTAATCGATGAATTTCATCAATAATGATAATAAAGTTATTTAAATCTAATGCTTGTTCAATGATTTGCATTAATTGCTTTTTTGAATCAACAACTGGATTGAAAAAAGCAAATGGAACTTTTAAATCTTGTGCTAAAACTTTAGCAAGCGATGATTTTCCTACACCTGGGGGGGCCATAAAAAATTAAATTAAATACTTGATGGTGATTAACCATTCTTCTAATTATACCAATTTCATCTAATAAATATTTTTGACCAACAATGTCATCAATACTATGAGGATTTTTTAGAGTTTTAATTAATTTATCTAACATATTATTTTTGATTAATTATTAATTTATATAATTCTTTACTATCAAAATTAGAAGATTTACTAACTTCTTTACAAGCATCTTTTAATTTAAAATTTTGTTTCATTAAATCTCTAATTTTAATTAGACATTCATTAATATTATTATTCTTAATAACATCTTGATTGATCTTATTATCAATAACAATTACAAACTCTCCTTTTTCAGTAATTGGGGGTAGTTGTGAAAGATAACCATAATAATGACTTTCATTTTTTTTTGTTAGTTCGCGCCCGATAAAAATTAAAATATCTCCTCATATTTCATATATATCCATTAAAGTCTTTTCTATTCGGTGCACAGCTTCATAAATAACAAAAGTTGTTTGCAAGTTTTTATATAATTTTAGTTTTTCAACACGTTGTTTTTGCGTTTTACCTAAAAAACCTAAAAACATAAAATCTTGCGAACACATTCCACTAGCAACTAGAGCATGCATCAAACTTGAGGGACCATTAATAACTTGAATTCCAATATTTTGTTGATGACATTCATTAATTAATTTATAACCAGGATCTGAAATTGTTGGATAGCCTGCATCACTAACTAAAGCTGTTGGATATTTTTTAATATAATTAATCGCTTCATTCAATTTTTGAATTTCATTAAAATTATGATAACTAATTAATTTTTTGTAATCATAAATATTTAATAGATGCAACAGCTTAGCTGTAACTCGAGTGTCTTCACATAAAATAACATAAGCTTCATTTAAACATTTAATAACACGTTCACTAACTTCTTCAAGATTGCCAATTGGTGTAGCAATAACAGATAAAATATATTCTTTATCACATAATTTCATAAGTTAAAATCCTTCAATAATTATTTTAATTTCTTTAAATGTTTTTTGCATCATGTTTAATCTTTTAAATAATTGTTTGTGATTACAATAACTAATATTTAAATAATCACAAAGCATTAAACGTATTTTTTTGTTATTTAAATTTAAACTTAAGTATTCTAATCAAGTAATAGATTCAATTGAATTAATAACGAATGTTCCTTGAGATTTCAAAGCTTTCAATAAAGCATCATTATTAGCTTCAGCAATTCCAATTTTTTTAGAATTATTCTTCATATTGTCCTTACTAATAAAACACTGTTTAATAATGCCATCTTTTAAATAATTAGTAATTATTTTACGAATTCTTTCTCCTTGATAATCAGGATCTAAAAATAAAATAATTCCTTTTGATAATTGTGCTTGTTTAATTAAAGCTAAAGTCTTTTTATTAATTTCTGATCCATTAGTAGTAATAATTTGTGCATTTACTAATTGATCAATTTTTTGTGCATCTGTTTTACCTTCTACAATAATAACTTCTTTGATAATTGGTTTATTCATTTTTATTTAATATATTTTTAGCAAATAAAACTTTTGAATTTTCATCATGTACAAACGACATCGAACCTGTACCCTCTGAATAAATTTCAATACATTTGGCAATTGCTGGAGCGATACTTACAATTTCAAGACCATCAAAAGGTTCATTTTCAATTGTATCAGCTATACAAATTTTATTAATAATTCGATCTTTAATTGCTTGTTGAAAATGTTGTCGTGCATTATTATTAAACAAACCATGTGTAGCAATAATTGTTATTGATTTAGCGCCTTTTTCACGCACAATTTTAGCAGCACCTAAAATTGTGCCACCAGTATCAATCATATCATCAACAATTACACAATCACGATCTTTAACATCACCTAATACATTATTAATTTCCACCTGATTTTTGCCAGATCTTCGTTTATCAACAATTGCTAATGTTGCACCTGTAGCTATTGAAATTTCACGAGCACGTTTGACACCACCATAATCTGGTGAAACAATAGTTATATTTGATGAATCTTTATAAGCACTAAAATAATGTTTCATTAAAACTCATACAGCCTCTAATGAATCAAACGGAATATCAAAAAAACCTTGTGTTTGTAAAGAATGAATATCTCAAGTTAATACACTAGTTGCGCCCGCACTCTCAATCATTTTTGCAACTAAACGTGCTGTAATTGGTTCACGTGGTTTAGCTTTACGGTCTTGACGTGCATATCCATAATAAGGAATTACAACACTAATTGCTTTGGCACTAGCTCGTTTAATTGAGTCAATAGCGATTAATAATTCCATTAAACTATCGTTAACAGGTTTAGATGTTGATTGAATAATAATAACTCGACGTCCACGCACTGGAACTTGAGGTGCAACAATTAATTCACCATCTGCAAACTTATCAATTCTAATTGGTGATAATTCAATTTTTAGTAAATTAGCAATTTTATTAGCTAATTGATGTGAATTTGAAAGACTAAATAATAATATATCATCATTTTTTGACATTAATTTTACCTACTTTAAATTATAAATTACTATAAACTTTTGTAAACAATTGACCAACCTGATATTCATCATTAAGTTCGACAATTCCTTGTGAATTAAAATTATTAATATTTAATTCTTTTTGTGAACACAACATTCCAAATGAATCATAGTTCATTAATTTGGATTTTTTAATTTCATTTCCATTTGGCATTAATAAATTAGGTGTCGCTACAACCGTTTTTAAGCCAATACGGACATTTTGTGCTCCACATATTATTTGTATTTGTTGATCACCTATATCTACTAAGCAAATATGTAAATGAGTATTGGGAATTAAATTAACTTCTAAAATTTTACCTACCACAAAACCTTTAAAAGCATTATCTAATGATAATTTTATTTTTATTAAATCACTTAATTTTTGAATTAATTGAGTCGTAGGATATAAATATCCTTCATTTAAATTTTTTAAATATTCACTTGCATTAAATAAATTTAAACCAATTAGTTCATCACCATGATAAAAAAGCAATAAGTCATCGTTTAAAAACACTTGATGATCATATTGCTGATTGTTTTTTAAAAAAATTAACGTATCGTTTAGACTTGTTTTATTATAATAAATATACATTTCAACTCCAATTTTTAAAGAGAAATTACTCTCTTTATTTATAAATTATAATATTAAATTAACTTTATCTAACTTTTAATCATAGTTAATAATGTTGTTTTTATTATAATTTTTAATAAGTTTTAGTTGATTAGGGGTAGATTTATGAAAACAGCAATTTTAATCGATACAGGTGCTAACGTCTTAGATATTAAAGATGATAATGTATATTTTGTGCCTATTCAGTTAATTATTAGAGAACAACAAATAGAAAAAGAATTAAAAGATGTTATTGACGTTAGTCAAGAACAATTGAAACAAATATTAATTAATAAAACAAATATTCATACTTCATTACCTTCACCAGGTTTTGTTATGAAAAAATTAGATGAATTATTCTTAAAATATGATCGTGTTGTTATTTTAACATTATCAAGTGGTTTATCAAGTTATTTTGAATCTCTAAGTATTATTAAAGAAGATTACAAAGACAAACATTTAGTGTGCATTGATTCACGTTCTGTTTCAATTGGTATTTTATGATTAGTTGATGAGATTAATTTACTCTTAAAAAATAATCCGAGTGATGAAGAATTAATTAGTTTAGTAAAACAACGTTCACAAAAAATTATAGGTGGAGTTATCGTAAATAATTTAGACCAATTAATTGCGGGCGGACGTGTTAAAAAAACTAAAGGAATTATAGCTAAAGCTTTACGCTTAAAATTAATTGTTCGTTGAAATGGGCAATTAGATTTTATGGATAAAACACCGAATTTATCAACAGCTATAGATAAATTATTAGATATAATTGATAATCAAAATCATTGGCGCACAAATGGTATTAAGAACATTGCAATTCTTACTGATTTAGAAAATGAATCACAAATCAATTCATTAAAAACTTCATTACAAGAAAAAATTCGACAAACTATTGAAATTAAAATTTCATATTTACCGGGTTGTATTTATGCGCATGTGGGTTTAAATAATTTTGCTATCTTAATTGAAGCTAAATAGACTCTTATTAAATAAAAGATCTGATAGTCTGTTAACCATCAGATTTTTTATTTAATCATTATTTATTTTAATTCTATTTATATTCGTTAGTTAAATTATTTTTTTTAGTTATTGCCGTATTAGATCTCTGTAATTCGCCTTTTTTATTAATTTTAATAAAATTTAAATTTTCATTTGTAACTACAAAGCCGTGTGTTTTTAAAATTGAATTATTTGGTAAAATAATAATACTTTCACCAACTTCAGGCTTACGAACTAATTTGATATAAAATGTTTTCGCTTGCTGATTGAATAAAATGCCTTCATTTACTTTTGCATTAAAAACTTGTGAGTTTTTTGTTTTATCAGTTGGGGTAATAGATGTTGAAAAAGTGACTTTTTCTAATTGTTTTTTTTCAAAATCTTTTAATTTTTCATAATTTGAAATTTGATATGCTACGTAATAATTATTATCTTCATCTTCAACTAATTTATCACTTAATTTAGATTCTATGTTTGAACTAGAGCATGAAGCAATTGATGTAATAACACTTACACCAATTAAAACACTACTAATTGTTAATACCCAAATTTTTTTGTTTTTTAATAATTTCATCTTGTTTTCTCCTATTTTCTAAAAATAAGTATGATTAAAAATATATAAATAAAACGTAAATGTGTTTAGCACACATCTACGTAAATATCAAAGTTATTTTGTGAAACTTTTAATAAAAGGATTTAGTATTGCTTTTTATACATTTCTTCACCATAAGCAATCACTTCTTCAACATCCTTTAATCCTGTTCCAGCAGGAATTAAATTTCCTAGAATAACGTTTTCTTTCAAGCCGATTAACATATCTTTTTGTGAACGCGCAGCAGCGTCAGTTAATATTTTCTTAGTATCTTGGAACGAAGCAGCAGATAAGAATGAACCTGATTTACTTGGCGCATGGTCTAAACCAAATACTTGATTAATTGCACTTGGTGGTTTTTTCTTATTAACAAGCATATTTTGAGCCACTTCAGTAAATTCATTAATACTAATTGTTTCTCCAACAAATAATCCTGAATCTCCTGGATTTGTAATTGTAATTTTATTTGTAAGTTGTGAAATGATTACTTCAATATACTTATCTGAAATCTCAATTCCTTGCATACGATAAACTTTTTGAACTTCTTTAATAATGTAATGACGCACATTTTCAATACCTGCATATTTTAGTAAATCATTAACATCAATTGATCCTTCAGTAATTTTTTGACCAGCAATAATTTCATCTCCTGTTTTAACACGTAATACAGCATTCGAACGTGTTGGATAATTTACATAATTATCTTTATTATATTTAATTACAACATTATAACCATTGGTGTTTGAATCTTTTTCGATTCGTTCAACAGTTCCTTTTACTTGTGAAATAACTGCTTTCTCATTTTCTTGTGGTTGAATGCAATCAAATAATTGTTTAATTCTTTCAAACCCTTGAGTAATATTCGTATCACCAGCAACCCCTCCAGTATGGAAAGTACGCATTGTTAACTGTGTTCCAGGTTCACCAATTGATTGCGCTGCAATTACTCCAATCGCTGTACCGGTTTCAATTAATTTATTTGTTGATAGATCTAAACCAAAACATTTTTGACAAACACCATAAAGTGATGCACAATGTAATGGAGAACGAACCTCAACTTCACGAATACCTGAATTTTGAATGATATTAGCTATTTCGCTTGTAATTAATTTATCACTTTCGATAATTAATTTATTTTTTGTATCATAAATTGTATTAATACTATATCTTCCAACAATACGATCTTTTAGTGATTCAATTGATGTATTATCTTTTGTATTTCGAATTTCACGAACAATAATACCTTCTTTTGTGCCACAATCATGATCTTTAATTACAACAGCCTGTGTTGAATCAACTAACTTACGTGTCATATAACCTGATTTTGCGGTTTTCATCGCCGTATCAGTCATCCCTTTACGTGCTCCAAATGATGAATTAAAGTATTCACTAACTGATAATCCTTCAATAAATGAATGCTTAATTGGAATTTCAATTGTATCTTTAATAACACCATTATTATTTTTTTGGTCATAGTTATATGATTTTGACATTAAACCACGCATTCCTGCTAACTGTGTAAAGTTTGACGTGTTTCCACGAGCTCCTGATTTTGCCATAATCACAATCGGATTATCTTTATATTCATTAGAATTAATTAGTTTTTCAATATCATGAGTAACTTTATCTTTTGTTTGTGATCAAGCTTGAACAACTTTAGTATATCGCTCATCATCAGTTAATTTACCATCTAAATAGAATTCACGAAGTTTTGAAACCAATTCATCAGTTTCTTTAAAATATTCATATTTTTGATCATAAGATGGAATATCAAAGGCAGAAATGCTTGTCGCTGAAATCATTGAATATTTAAATCCTAAAGCTTTAATTTTATCCATTGTTTGTGGTACGACTTCGATTTCAGAAACTTTATATAAATAATCAATTACTGTTGATAAAGTTTTTTTAGTTAATGGTTCTTTTAAATTATAGGCTTTTAAATAATCTAAAATACTTTCATGCATTCCAATAATTTCAAATTGATTTTCGCCATATAGATTTTTAACATCATTAATATATGGAAAATTAACAGGAAAAGCTTGGTTGAAGATAATTTTTCCAACCGTTGTAACCAAAATACCTTCTTTACTAAATGTTTTATTGTTAAAAGCTTTTGTTGTAATTCCAATTACGTCATTAATACGAATTGTTTTTAATTTATAAGCACGAATAGCTTCGTCTTGTGTTGAAAAAATGTGAAAATGATTAATAAATTCAACTCTCGCTTGCGTTGGATCTTTAGCCATCATTTCTTCAATTACTTGTGGAAATTTTTCTTTTGTTAAATAATAAATACCTAAAATCATATCCTGTGTGGGTGTAATGATAGGTTTCCCATCTTTTGGACCAAGAATATGTCATGAGGCTAATAAAATTGATCGTGCTTCAGCCACTGCTTCTTTGGATAAGGGGATATGAACAGCCATTTGGTCTCCGTCAAAATCCGCATTAAAAGCGGTTGTAACTAGAGGATGTAAACGAATAGCTTTACCGTCCACCATTTTTGGTTCAAAAGCTTGGATCCCTAAACGGTGTAAGGTTGGTGCACGGTTTAAAATTACAGGACGTTGCTTAATAACTTTTTCAACAACGGGTCAAATCTCATTATCTTGCGCAAGAATTTTTTGTTCTGCTAATTTAATATTGGCACAAATTGGTTGAATTTCATTACCTAATTCATCACGTTTACGTATTAATTCAGAAATAATATATGGTCTAAATAATTTTAAAATCATTAAAGCAGGAATTCCTACTTCATACATTTTTAATTCAGGACCAACAACAATTACAGAACGACCTGAATAGTCAACACGTTTTCCTAATAAATTTTGTCTAAATAATCCTTGTTTTCCTTTAAGATGATTTGACAATGATTTTAAAGGACGTTTATCTTTACCTACAACTGGTTTTTTACGTGAATTATTATCAAATAATGAATCAACTGCTTCTTGTAATAAACGTTTTTCATTATCTAATAAAATTTCAGGAGCATCTGTTGCTTGCATTTGTTTTAAACGTTCATTACGAATAATGATTTTACGATAAAAATTATTTGTGTCAGAGGTCGTAAAACGACCGCCATCTAAAGAAATAATTGGTCTTGTTTCAGATGGTGTGACAGGGATTCTTGTCATTACCATTCATTCTGGTTTTGAACCTGAATCCTTAATTCATCTTACACATTCTAAACGACGCAATAAGCGTTTTGTTTTTTGATCATAAGCATTAGGTGAATTTTGAATTGCTTGTTTAATCTTTTCATGTTCTTCTTCAAGATTTAGTTTTTCTAATAATTCACGAATAGCTTCAGCACCAATACCAAAACGAACGCCTGTATGTTTTTCAATAAAAGCCATTACATCTTTCACTGAAAAAGGAATATTAGAATCTTTTAACATTTGATCATATGTTACAGCTAAACGATAATCAAAATCTTGAATTAATGCTTCACGTTCATGATTTAATTCATCTTCAATTCGTTCTTGAATATTACGTAATGTTCGACGTAATTTATTACGTGATCGAATTGAATTTTTTGGTGATGTTAAATCTAAAACTTCTTTTTTATTAAAAATTGATTTACCATCATATACATTATTTCCTTCATCTAAAACAATGTAATTAACAAAATAAACTACCTGATCAACTTCTTTATATGTAATATCTAATAATAATGAGATTTTTGATGGCGAAGGTAATTCTTTTGTAAATCAAATATGGGCAACAGGAGCAGCTAACTCAATATGTCCCATTCGTTCACGACGTACAATTGATTCAGTAATTTCAACATGACAACGTTCACAAATTTTACCTTTATGTTTTACTTTACGATATTTTCCACAATAACATTCATAATCTTTTGAAGGCCCAAAAATACTTTCATCAAACAACCCATTAGGTTCTGGTTTTAATGATTTGTAATTAATTGTTTCAGGTTTAGTGATTTCACCTTTTGATCAATTTAAAATTTGTTCAGGTGAAGCAATGGAAATCGTTAATGATTTAATCCCTTTTTGACTCATATTATTTTACCTTTCTATTATTTAGAAGTTCTCTTCATCATCATAGTCATTATTATTAAAGTTTGTATTAAATATTTCTTCATTAGAATCATTGATCTCTTTTAGATTTTCATCTAAAATAACCTCATCATCGTCATTATTTAAACGATTTTGATTTAGTGTATATTCATTAATATCTACCATACGATCATCTTTTGTTTCAACGGTAATACATAACCCTAAACCTTGCATTTGTTTTGTTAATAATTTAAATGATTCTGGCATTCCATCAGCAACAACATCGTGACCTTTTATAATAGCATTGTAAGCTTGATTACGTCCTTGAACATCATCGGATTTAATTGTTAAAATTTCTAATAAATTATAAGCTGCACCATAAGCTTCTAAAGCTCAAACTTCCATTTCACCAAAACGTTGGCCTCCATTTTGTGATTTACCACCCAATGGTTGTTGTGTAATTTTACTATATGGACCAACCGAACGACTGTGAATTTTATCATCAACCATATGATCTAGTTTTAACATGTATGTTAATCCAACTGAAATTAAACCATCGAATGGTTCACCAGTTCTTCCATCATATAGAACTTGTTTACCCTTATTATTTTCAATATCAATGTTTGCTTCATTCATAATAGAAACAATATCATCATGATTTGCACCATCAAATACAGGAGTTGAAATTTTAATTCCAATATCATCATATGATAATCCCAATTCTTTTAGAATAATTGTCACATCAATTAAATCTATATCTTTTGCCTGTTTTGCTTGTTTGTGTTTAATTAAATTACTAATATTTTCATAAAGTTTTTTAGCAATAATTTCATTGATTCCAAACAATGAAATATATTTTTCATAACCTAATTGATCAATTGCAATTTGAATTAATTGTTTTTTACCTATTTCAGCCGCAGCATAACCTAAGTGCAATTCTAAAATTTGACCAATATTCATACGACTAGGTACACCTAATGGATTTAACATAATATCTAATGGAGTCCCGTCTTTTAAAAAAGGCATATCTTGAATAGGAACAACTTTTGATACAATTCCTTTATTTCCATGACGCCCTGCCATTTTGTCACCAACTTGAATTTTACGTTTTTGAACAATATAAACTTTGATAATTTCAATTACGTCATCTGGTAATTCACTACCATTTTCATCACTTGATTTAATTCGCTTAACAGCAGCAACAATTCCTTCTTGACCGTGTTTTACTTTTAGTGATGAATCTTTAACTGTTTTTGATTTATCGCCAAAAATTGTTTGCAATAAACGATCTTCTGGAGCAGTTTCAACATTACCTCGTGGAGTTGTTTTTCCAACTAGAACATCTCCTTCATGAACTTCAGCGCCAACTAAAACGATACCTTGATTATCTAAAAAACGTTTTGCAGTATCAGAGACATTAGGCATGTCACGAGTGATTTCTTCATCACCGTTTTTTGTTTTCATACATTGAATTGTATGCTCATCAATATGAATTGATGTAAATACATCTTGATCAACTAAACGTTCAGAAATAATAATAGCATCTTCAAAATTATAACCTCGTCAAGTTGTATAACCAACTAGAATATTACGACCTAAAGCTAATTCACCATTTTGCATTGCAGGTCCATCACCAATTGTTTCAGATTTGTGAACTCTTTGACCTACTTTTACAATTGGAATTTGGTTATTACATGTGTCTTGATTTGATTTACGATATTTAATTAATTTATAATCATCTAATTTATCATTATCATTTCTAATGATAATTTTTTGACTATCAACAAATTCAACCACTCCGTCATTTTTAGCAACAACAGCCATTCCTGAATCGTGTGCAATTTTAAATTCTGTACCTGTCCCTACAATAGGGGCATATGGTTTAATTAAAGGTGTTGCTTGACGTTGCATGTTTGACCCCATCAAAGCACGTGCCCCATCGTCATTTTCAATAAATGGAATTGCTGATGCAGCAATTGATACTACTTGTTTTGGAACAATATCAATAAAATCAACTTCATTAGGACTAAATAAACCTGTTGAGCCACGATATCGAGCAACAACTTGTTCATCTAAAATTCGTTTATTTTCATCTAATTGGACTGATGATTCAGCAATAATATAGTTATCATCTTCATGGGCTGTTAAATATTTATAATCTTCTTTTACAACCCCATCCTCAACAACATAATAAGGTGCTACAATAAAACCATTTTCATCTACTTTTGCAAGTGATGCTAAAGACATAATCAACCCAATATTCATTCCTTCTGGTGTTTCAATCGGGCAAATACGCGAATAGTGAGAGTGATGAACATCACGAATATCTAAATTAGGGTCTTCACGACTAATTCCTCCTGGACCCATCGCTGAAATACGACGTTTATTAGTTAATTCTGCTAATGGATTTTGTTGGTCAATAAATTGAATTAATTGATGTGAATTAAAAAAATCTTTAACTAAACTTTGGAATTGTTTTGTATTAATAATTGATTTAACACTAATTGCTTTTTTCTTTTCCTCATCACTCATATCGCTTTCTTCAATTTCACGATCAAGTTCAGTATCAATACCTTTATCATTAACATTTGTAATGTTATTGCTTGAACCATCAGAAATAGCCAACTTTTCATTAATAAACTTCTCAATACGAGCCATACTTGTAGCAATTCTTGCTCGTAATAATTCATGAATCAATTTAAGTCTTTTATTACCTAAATGATCTATATCATCAAATTCACCAATTTCATAATTCAAGTTATAAATATAACTTGTTGTAGCAACTAAGTCAGCCACGGTTAAAGTTTGAAGTTTGCAACCTGATGCTAGACCGACAATCTCTGTTGTTTCATCTCTTAAATCATTATCTTTATAAACTGCAATACTTTCATATTCTAAAAATTGTTCAATTTCTGATTCTAATGCTGTTGGTAATAAATTAATTTTTTTTGTTCATTTAATGTTTTTATCACGTGATTCTTTTTTTATTAGTTCGATATGTTCTTTAGTAATTAAAGTATCTTTAGGAATTAGAATATTATTTTTTTTATCAATAATATCATTCGCAATTACTTTTTGATATAAACGTTCACTTAATAATAATTTTTTTTCAAATTTATAGCGTCCAGCACTACTAATATCATATAAACGCTTATTAAAAAAATGTTGTTGTAAAGCTATTTGATAAGAAGCTTTGCCTAAATGTCTTAGTGTTTCTATATTTTTAATATTAATACCTAATAATTCAACGATATCCTTAGCTGCTTTTTCACTAATGATGCAATTAATGATTTCTTCTCTTTCATCATATAATTTTTTATTATTCTTAATGAAACTAGCATCTTCAACATCTTTATTTTCATGTAATAATGCATCAATTTCATCGTTTTTTTCAACATAATTAAAAATTAGTTGTTTTAACTTTGATAAAAGTGGTGAACCTTGTTCTATATATTCTTCTTTTAACTTATTATTTGATTCTCGACGATCGGCTTTCTCATGAATATCATGAGCATCACTATAAATTTTAAAAATAATTTCATTTTCTTGTGAATTTTCAAAAATATATTCTGGCGCATATTTTTCAATTTCTAATGATTCACGAATCTCTTTTTCATTATTAAAAATTTTTAAGATTTCAGCACTTGTTAAACCAAAAGCTTTTAGTAAAGTAGTTACTGAAAATGTTTGCGCATTATCACCAGATGAATCACGTGCAACAATTTGAATATTATGACGATCCTTAGGAATATAACCAAGCATCAAAGTACCCTTAGAAGGATAAATTTCACAAATTTTGCCTTCAAATAAACGCTTACGTGAACCGTTCAATTTGATGCTTGATTTACCAAGTACATAAATTCCTGGTGATCTAACAATTTGTGAGATAACAAATTTTTCAATTCCATTAATAACAAAAGTTCCTTTTTCTGTCATTTTGGGAATTGCTCCTAAATAAATACCATCTTCTCCTGTTTTAGTATTTCGTTGTGCACGTTTAACTTCACCTGTGTAGTTATCTGTTAATTCCAAATCAATATAAATTGGTGTTTCAAATGTTTTACCTTCGTCACGACAAGCACTTTCATCTTTTGTTGGCTGCTTAATTTTTAAACCTTTAAATGCTAACGTATACTTACCCTGAGGAGATTTTAATGGAAAAATACTTGAAATAACTTCTTTTAAATCTTTCTCCATAAAGCGTTTAAAAGATTCAACTTGAATTTCAAGTAGATTTGGTCCTTCAAAATTAGATTTAATTTTTGAATAATCTCTTCGCATTACTTTATCAGTAATAAATTTCTCAGTATAATTTTTATTATTTTGCATATTCTAACTAGTACCTCACAAAAATAGAAAAAAGCCTATTTATTACACATTTGTGCTTAAATAGACAGAATATTTTATAATTATACTATATTAAAGATATTAATAATAAAATTATTTTTATTTTAAATTGAAATAAATAAAAATTCATGATTATTAAAATCAATATTAAATGATTTTAATAATTCTTCTTGTTTTAACATTTTATTTTCACTAATAATAAAATTAGCATTTTTTAATAATAAATCATGTTCTTGATTTTTATCTATAACAAATAAAATATTAAAATTAAAATCATATTTTAATATATCATAAACTAAATGTTTAGAATTTTTACTAATTAAATTAACAACTAATAATTTATGTTTATTTATTTTTAATTTATGAAATGATAAAATTTTTAATTTAACTTCCTTAGGAATTAATAATTCATTTTTTTTAAATGTTATTTCGTTTTCTATATTATTATTTTCTGTCTCTAAAATAATAGATTTCATAACTTGTACAGCATTAATTGGATCAATTCCTGCGGCCGTTTCGCCTGATAACATTGTTGCGTTAACCTTTTGTTTAACGGCATAATAAACATCTGTGACTTCAGCTCGTGTGGGGATAATATTACGTTCTAATGAATCTAACATTTGTGTCGCTATAATACAATATTTATTGTATTTATGGCATAATTCAACAATTTTATTTTGATAAATAGGAATTTTATAAAAAGGTACTTCTAGCCCTAAATCCCCACGCGCAACCATGATACCATCAGTATTTTTAATAATTTCTTCTAGATTATCAATTGCTTCTTGAGTTTCAATTTTTGCAATTAATTTTGGTATAAATGAGGACTTTTTTAAAAGTTGTTTAACCTCATTAATTTGTTTAATATTAGAAATAAACGATAAAGCCAAATAAGGAATTTTTAATTTAACTGCTAAATTTATATCATCAATATCTTTCTTTGACAAAAACGGCAAACTATAATTGGCATCTGGTAAATTTAAGCGTTTATTTGTTTTTAATGAATAACTATTTTTAGCGGTTGTATAAATGATGTTTTTTAGAAAATCAATTTTTTTAACAATTAAAATTAATTTTCCATCGTCGATTAAAATTCGGTGATTAATTTTAACATCACTAATCATATTGTATTGTCCAGTAGCATCAGTAACACTAAACTCATTAAAACTACCTAAAACTTTTTCTTTACAATGTATTATAACTTCACTATTTTTAATAATAAAATTATTATCACTCATTTCACAAACACGAATTTCTGGTCCTTTTGTATCAAATAATATTTCAACTTTGATCTTTAATTCATCCGCAATTTTTTTAATTAATTGTGTACGAAAAAGGTGTATTTTTTGGTCGCCATGACTTAAATTCATACGAAAAATATTAACACCACTTAAAATTAAATCTTTAATGTTTATGCATACTTGTTGAGCATCATTTATATTATTTAAATCGTTAAATTCTAACCAATTAGAAAACAATTTTGTTGTTGCTGGACCTACTGTAGCAATAATGCGTGTTTTTGAAAACATTAATTTTCCCTCTATTTATTGAATTTAATAACTTCTAACTTATTATCAAACTTATTAATAACTACATACATATTATTTGTGTTTTTTATATCATTTTGATTAACAATAAAATCAACCAATACATTCGCACTATCTTTTGCTAATTGTAAATCAAAATCACTTACTGGAGCTCCGCGTTGTAAATAATTTAAAATTGTAATTCGTAAACTTTTATGTAATTTTTGCTCAAAAGTTTTTACTAATAATTCGTATCATTTTTGATCATAGATTAATTCTTGAACAATGATTAAAGGATCGTATTCTTTTATTGAGTTTATTTTATCTTTAATTAAATCTATTACTTCATCAACAGTATATTTATTATCTTGATTAGTTAACATTAAAATAGGTTTTAATTTACCAACACTATTATTGACTAATCATGAACATTCACGACCCATAAGCTCAACTAAATTAAAAGCACAATGAGCTTTTGCTGTGTAATTTAGGCACTTTAATAATTTTTGATTAAAAGAAGCAGCACTTAAGGCCCCTATACACATTTGTGTTTCACTAAAATCATTATCAATAGTGTGTAATATTCCAAAGACTTCAAAGCCCATTTTTGTTAACACTAAACCAGCTTGCATACTACCTTGGCCGCCTAAGATAATAATTTTTTGAATATTATGACTTAATAAATTATCTTTAATAATTTGTGTTTTTTTATCATTAATATCTAATTTAATAAATCTACTTGTCTTAATGACTGTTCCTTGCTCGTTAAAATAAACATCAAAAACACGTTTATCTTTAATTGGTTCAATTAAATTATTGTACAGTCCTAATAATCCTTCAATTCCTATAAAAACATTAAAATTATTATTCATTAATCTATGGATTAATGAAATTAATGAAGCATTCATACCCGGTGCATCACCACCTGAAGTAATAATTAAAATATTTTTATCTAAATTTAAAAAATTTACTTGGTTCATAAATTAATTTATCCTTTAATTCTTGTTCGATTTCTAAAATACGATTATACTTCGCTATCCGTTCAGAACGTGATAATGAACCTGTTTTAATTTGCCCAGCACCTACACCAATCGCTAAATCTGCAATAAAAGTGTCTTCAGTTTCGCCAGAGCGATGCGAAATGATAGTATTAATATTGTTTTTTTGTGCAAATTTAATAACATCTAACGTTTCACTAATTGTACCAATTTGATTAGGTTTAATTAAAATAGCATTTGTTGCTTTAAAATCAATTCCTTTTTGTAGATACTTTAAGTTTGTGCAATATAAATCATCACCAACCACTTGAATGTGTGGGTTATTTTTTAAAAATAACGCAAAACTATTTCAATCGTTTTCCTCAAAACAATCCTCGATCGAAATGATAGGAAACATAGATACTAATTTACTTCAATACTTTATTCATTCATCACTACTGAATGATGTTTGTTCAAGAGTAATGTTATGAGATGAAGACTTTTTAAAAAAATATTTTCCGTTTTCATACAATTCAGAAGCTGCGACATCTAAGGCAAGACAAACTCCTTGTGTTTTTGAAGGAAGGTAATGTGCTTTTTTAATAGCATGAACTAATAGTTCTAAAGTCACATAATTATTTTTTAACATTGGAGCAAAACCGCCTTCATCACCCTTTGTTGTACTTAAATGATTGCTTTTTAAAAGTTTATCTAATTGATGAAAAATTTCTGATCCAATTTGAATCGCTTGAGAAAATGAAATTGCATTTAATGGAACAATCATAAATTCTTGAATATCTAAATCATTATCTGCGTGAGCCCCACCATTAATAAAATTCATTAACGGAATTGGAGCATAATAGTTTACATCATAATTATGCATTAAGTCTTCTTTAATATATTGATATAAAGGTTTAGAAGCGGCTATTGCTCCCGCTTTAACAATCGCTATAGAAACACCTAATAAAGCATTAGCACCTAATTTACTTTTATTTTCAGTACCATCTAGATTAATTAATAAATTATCTAATTCAAAAAAATTTAAAACATTTTTATTAATTAGATGAGGGCGAATAATATTATTAATATTTTGAATTGCTAATTTAACAGACTTATTAAAAAAATAATTTACATCACCATCTCTTAATTCTAATGCTTCCTTTGCTCCAGTGGAAGCGCCTGATGGAACCATTGCAATTACACTTTGATCGTTCTCTAAAAATAATTTAACAGCTACTGTTGGTTGGCCGCGCGAATCAAGAATTTGATAAGCTAATAAATTAATTATTTTCATATGTTGTTAATTCCTCTTTTTTAAAAGACTTATACATTAATTTTATGTTAAAAATTAAAAAAATATTAAGACAAATAATTAAACAAGTGTCTTCAATATTTTTTATAAATCTAATTCTTTAAAAATTTCTTCTAAAATAGGGTCAGGAGCAACAATTTCTTGTTTATTTTTTCTTTTTTTTATATTTAAACCATCTTTTCGTTCGTGTTGTTTAATTTTTCAATTGTCATTAACTAATAAACATCGTTTAATAATTTCACTTAGATAATAAATAAAACTAATGATAAAAATTATTCCAATAATTGTATAAGTACAATAAATTAATTTTGCTTTTGTAAAATAAAGATTGGCATTATAGTTGTATGAAGGATTAAATGTTAAATTTTCATTATTAATTTGGTGTTTGCTAATATCTAATAAAGCAACTTGTGGATCATTAATTATTGGTAAATGATAGTGATTAATATAAGTATTTAAAATCACATTTTTTTTATTAAGTGTTAAAGTTTGATCATTAAAAATAACATTTTTTAATCATGTATAATAACTATTATTAATAATCTGATTAAAGTTTGGTTTGATCATAATAATGATAAATAAATATAAAATAGCAAGTCAAATTAATATAGCAATTATTAAATGTAATAAACTTGGCCTTCAAGATCTAATAATTCCAATCATTATTAGATTAAAAATAAATGTTAAAATATAACAAATAAATAAAACAATACTAGCTATACCACTTGCATTTAAGGCAATATTAAAGTTTAAAGTATAATTTAAATAAAATAATGATCGAGGATCAATTTTATAAGCATATGTACTAGATGAATTTATTCATTGACCAACTCGATTAATTAAAAAACCAGTTTGGTTAGGAATTGTGATTGATAAAATGAAAAACCAATGCAAACAAACTAAGCTAAAAACAATTAAAATAATGTGTGATCAAGTATAAATTTTAGCTTTTTTATCCATTATAAAACTCCTTCGTATATCAAATTATTATAATAGCATAATAAAACTATTCACAAATCTTTTTTATCATACTCGTTGCTGTCATTTGAATTGGTTGAGCGATATTTAAATATTCCAAAATAGTTGGAGCAATATTTGCTAAACTAAATTTTTGTGTCGAATTTAAAATAACTTTATTATCTGTGATAATAAACCAAACATCATTAACACTGTGAGAAGTAATTACTTGTTTTTGATCATCAAGCATCATTTCAGCATTTCCATGATCACCTGTAATCATCAACACACCATTATTCTTTTCAAAAAAATCATATAATTTTCGAATTTCATGGTCAACACTTAGAATTGCTTCAATTGTTTGCTGCATATTGCCGCTATGACCAACCATATCAGGATTTGCATAATTAACAATAAAAACATCATGATCGAAATAATGATCGATAATAGTTTTCGTAATTAAATGTGCTGACATTTGGGGACATAAATCATAAGTAGCAACTTTTTGCGAAGGAATCAAATATTGTGTTTTTGTCGAATAATATTTATTAACACCGCCATCAAAGAAAAAAGATATATGGGCATATTTTTCTGTTTCTGCAATTCGAGCTTCTTTTATTTGGTGTTTATTAAAAACATCACCTAAAGTGTTATTTAAATTTTGCTTCTCAAATAAAGCTATTGCATCAACATCAGGGAATGGTACTAAAGTAATTAAATTAACATCTTGAACTAAAAACGGATTATTGTAATGAAATTTTTTGTTAGGTTTAAGTAAGTGTGCTAACTGGCGCATACGATCTGAACGATAGTTTGCTAAAATCACTTTTTGATGTGGTTTTAAATTATATTTTTCATCATCATTAAAAACTACAGGTTGAACAAATTCATCACTTTTATAAGAATTTGCGATTGATTGTGCAACCTCTAAAAATGTTTTTTCACATATTTTATCTTGCTTAACAAACATTGCATTAAATGCTTTTTGGGTACGTTCTCATCGCTGATCACGATCCATTGCAAAATAACGCCCACTTAGACTCTTTACAATAATATTATATTGTTTTTTTAAGGCATTAATTTGTTCAATGAATTGAGCAAAACCATAAGGATAGTCATCTCGTCCATCAGAAATTAAATGTAAAACAACCTGATTATTGTTTTTAATTATTTGTTTAATAATTCAATGAATATGTTCATAATTACTATGTACTAAACCATTCGAATACATGCCAACAATATGAACAATTTCATTTTTAATATTGCTTAAAACTGCACTTTCATAATCAACGGATTTCATATAATTATTAATATTTGCATTATCATTTAAAATGATACGGCCCGCACCAATTGTTAAATGACCAATTTCGCTGTTACCTGCTTGATTTTCAAGTAAACCAATATTTTGTTGAGCCGCACTAATTTGTAATGTGGGAAAATTATTAATCAAATAATTTAATGTTGGTGGATTTGCTAAATGAACAGCATTAGTATTATCTTCTTTTCCAATTCCTAATCCATCAATGATTATCAAACCTATTTTTTTATTTAATTTCATATTCTAATCCATTTTATTATTTACATTTATCGATAATTTTATTAAAAACATTAACATCTAATGAAGCATTGCCAATTAAAAAACCATCGATTAATTTTTGATTGCACAATTCATTAATATTATTGTCATTAACACTACCGCCATATAAAATAGGAATATTTATTCCAGTACAATTATATAAATATGTTTTAATTCCTTCGATCATGTAATTAATATGTTTAAAATCTAATTCTAAATCAGTGCCAATTGCTCAAATTGGTTCATAAGCGATAATAATTTTTTTTAATCCTTTGTAATTAATGATGTTGGCTAATTGATTTAAAACAAAATTAAGTGATTTATTTTGTTTATAAGCATCAAAAGTTTCGCCAACACAAATGATGACTTGTTCTAATAAATCCATAGATATTTTTATTTTTTGATCAATTATTGATTGTGAAAAATCAATACGTATTTCACTATGCCCAACTAAAGCATTTTTAATATTAATATCTAATAATTGTAAACAACTTATTTGACCTGTATAAGGTCCTTTATTATGTAATGATGGGTTTTGAGCATATAAATTGACTTTTTTTATTTCAGCTAAATTAACAAATGTTTTTAAATATAAATCCCCTGGTGCTAAACCAATAATAATTTTTTGCTCATCAAATAACGTTAAATTATTTAAAAAATGATTAATTAATTCTTGTGTTGCATTCATTTTAAAATTAGCAATAATATATTTCATTGAATCATTATCCTTTATAATCTTAGTTAGATTATAATCAAAAAAAAGGTTATTTAACTAAGATGATGATAAAAATTATAAGTGTAGGGAAATTAAAACAAAAAGCTTTTGTTGATCTAATTAATGATTATTTAAAAAGAATTAACCATTATTTAAAATGCCAAGAAATTGTTGTTAGCGATGAACCTGAACCAGTTCAAATTTCAAATAAATCCTTAGAACAAATTAAAAGTAAAGAGGCCTCTAAAATTTTTAAAAATATTAATCAAAATGATTTTGTAATTGCTTTAATTATTGAAAGCAACATTATAAGTAGCGAAACATTAGCTAAAAAAATACAACAATGACTAAACACATTTTCTCATGATATTTGTTTCATTATTGGTGGATCTAATGGTTTACATGAAAGCATCTATGAGCGTGCTAATTATCATTTATCTATGTCAAAAATGACTTTTGCTCATGGCTTAGCAAAAGTCATGTTATGTGAACAAATATATAGGGCACTTAGTATTTTAAATAACGGAAAATATCATAAATAAATTAAATGTTTTGATCATATTCTTCAAATACTGAACAACCTTGTTGATCGTATTTAATTAGATTAATAAATTTACCAACTTCTGGAATAATATTCTGCAAATCAAACTCGTAAATTTGAATATTTTTTTCGTCTTGATAATCATTTTTTAAAGTTACAACACTATCTTTTATAGCAATAATTGTATAAATTTTTTCACTGCGTTTCGCGGGTTTAATTAACATATTTTTTTAATGTCCTTTAATAACTAATTTATGAATATAATATTTGTATATGTACATTAACAAATTAAGTATATAGTATACTATGAAAATAAATCTTTTTTAATTAATTCTTTAAGTTCCAAACAAAAATCACCAAAAATAATAGAAATTTTTGTTTGATTGCACATAATCCCTTTTGCACCTAAAACCTTTAATTTTTCATTATTCAATGCGTTAATTTCTTTTAATTCAATTTTTAATGTATTAATTGTCGCATCAACGTTAACTAAATTATTTTTTGAACCAATACAATTATAAAAATCATTTAAACTAAAAGGAATATTTGAAGATACTATTAAATGAGTGTTTCTAATTTGAGATACTTTTTTAGCTTTTGTAAAAAAGTAAATAATAAAAATTCCAAAGGTAATAATACCTAAAAAAATAATTAATGTTTTTTGCTTATTATTTAAATTTTGCATTTTTATTCTCCTTTAATAAAAGATTTGTTTAATTTATCAATGTATTTCTTAAAATTATTTAAACCATGAATTTTTGATGAAGCTTGGATTGTTTCTACTTTAATATCATTAATTTTATCGCTAAAATTAATGATATTCCCATCAATAGCAACACTAAAAGATTTTTTAAATTTAATATTTGTTAAACTGATTTTATAATCTTTTCTAAAAACCACAGGGGCATTTAAACTTGAATATTTTAAATGATTAACAGGAAAAATCTCTGTCATAATAAAATTCTCATGATTATTAAAAATAATGGGACCAAAAGCTGTTTTGTTTTTGCCAGTTGATCCGCTTGGTGTTACAAATAACAAACCAGAACCACGAAATTTTTCATATGGATAATCATTTATGCTAATATCAAAAGACACTAACTCATTAGAAAATAAACTTAACTCATTCAAACAATAAAAAAAATTTTGGTTATTAATACTTACTTTAAGTAATAAAGGATTATAATACATATTTTCATCAATAATTTCGTCTAAATTTATAGTTTTAATACTATCATATGAACTATAAAAACCTAATTGTCCATAATTAATGCCAACAATTTTTACATTTTTTTTTCATTGTTGGTTAATAAAATTGATAAACGATCCATCTCCACCTAATAAAAAAATAATTTCAGGATTTTCTTTATTTCGTAAAAATGTTATTTTTGAATATCTTTTTAGTTTATCTTCTAACAAATTAATATCATTTCTTTTAAAACATTCTTGTGGTTTAAAACAATAGATATCATAAAAACAAATTGGCTTATTATTTTTCATGCACTGCTCGTTTAAAAATTTCAATTTCTTCTGAATCATAACCAATTTGTAAACGATATGGTCGATTGTTTTTTGTTTGATAGATTAAAGGGCGACGAATTAAATTTAAATCATTTTGAATTAAATTTAATAATTCTTTTGTAGATAAATCTAAAAAAGTTTTTGAAGTAATATTCAAGCGTTTTGAAGATTCTGTCTTAAAAGATATAATTGTTCCAAAGCCATCTTCACAAAGAGATAAAATATCTAACAACATAGAACGTTCTATTTTATCTTCACCAATAATAATTTTGGTAATTTCAATTTGATTATTTCGGAAAAATCGCACAACTTTTTTACACACTGCGCAATTAGGACTGTATAAAACGTAAATCATATTCCTTTTATTTTTATCTTCTTATAATTACAATTTTACTTATATAATAATTAAGTATACAAAAAAAACATTAATTTTAAATGTTTAATTGTATTAACGTTTAAGTAAGGTAAAATAAAACATTATGAAAAGACTAATTTCAGGAATTCAACCAACTAATAATTTAACATTAGGAAATTATTTAGGTGCAATTAAAAACTTTGTTGATTTACAAGATGATTATGAAGTGTTTTTATTTGTTGCGGATTTACATTCCTTAACACCAAATATTTTTGATAACACTGATTTTTCTACTACTAAACGTCAGATTATCGCGACATATTTAGCTGCTGGTATTGATCCTAAAAAAACATGTCTATTTTACCAATCAGATATCTTATCGATTCCATTATTAGCTCACATATTATTATGTTCAACAAGTATTGGTGAACTAACGCGTATGACACAATTTAAAGATAAAAGTATTAAAGCAACAAAGATGGCTAATAACACTGAAATGATTCCTTCTGGTTTATTAACATATCCCACATTAATGGCAGCAGATATTTTAACTTTTAATGCAGATGTTGTTCCTGTTGGCCAAGATCAAAAACAACATTTGGAATTAACAAGAACGCTTGCTGACCGTTTTAATAAAAGATATGGTAATACTTTTAAATTGCCTCAAGTTTATATTCCTAAAATAGGGGCAAAAATTATGGATTTATTAGATCCTAATATTAAAATGTCAAAATCTTCTAAAAACCTTAAGGGTGTGATTTTTTTAAATGATTCAAAGGATATAATTTTTAAAAAAATTAAAGGAGCTTTAACAGATAATTTAAACAAAGTTAAATATGATTTAACATTACAACCAGGAGTGAGTAATTTAATGACAATTTATGCTTGTTTAACAAATCTAAGCTTTAAAGAAATCGAACTAAAATACCAACAACAAAACTATGGTGTTTTTAAAAATGATTTAGCCAATATCGTTGCTGATTTTTTAGAAAAGCTGCAACAAAAAATTTCTTATTGATTAAATAGTCCAGAATTAGATATAATGATAGACAATAGTTGCGAGCGTGCTAACGATATTGCTTATCAAAATGTACAACTAGTTTTAAAACATATGCAACTAAAATAATAGTATATAATGAGGTAAAAAATTAATGGGAAAAAACAACAAATATTTAATTGCTTGTGATCTAGATGGAACACTTTTAAATAATGATAGTGATATATCAGAAAAAACAATTGAGGGAATTAAAAAAATCATCAATCAAGGCCATATTTTCTGTATTGTAACAGGTCGTCCCTTACGTGGATCAATCCATATTTATGAAAAATTAGGATTAACAACTTTAATGGCTAATTATAATGGCTCTTATATTTCAAACCCTTCCGATAAAAAATTCGCGCCAATTAATATGACATTTAGTAAAGATATTGTAAGCGCAATTTTAAAAAATCGATTTATTGCTAAAAATATTGTTAATGCTGTTGTTGAAGCTGATAATGTTGCTACCATTTTAAACAGTAATATGGATTTAAATATTTGAAAATCATTTATTGATATATTTCACGTGCCCATTCGTGATATGAATGAAAAAAATAAATTGACTAAGGAAAATGATATTAACAACCTTATTAGAGATGCTAATGCCCTTTTATTAAATGTCAACGATAAAGCTTTATTTGATTCTATGGTTTATCATATTAAACAAATTGCTCCCACTTTAGCGGTTCGTAGCTGATCAGCGAGGGTTGATGACCAATTTGAAGTTATTGAAATTAATTCATCATTTGCTGATAAAGCAATGGCTATGCGCTATTTATCCTCATATTATGGAATTCCATTAGATCGTTGTATAGCTTTCGGCGATGGTGAAAATGATTTGACAATGCTAAACCATGCTGGATATGGCTTTGCCATGAAAAATGGTACACGTCCCGCTAAATTACTAGCACGTCACATTACTAAACACACAAACGTAGAAAATGGTGTTGTTTGAGAATTAGAATATGTTTTAAATGAAAATAAATAAAGTTTTTTTAAATAATGTTCGTTTATTGAACATTATTTTTTTATATATAATTAATGTTATAAAGTGAGGTTATCAATGCGCACAGGGAAATACATTACTAAATATGATTACATTGGTTATTATACAAAGCAAAAAGCAATGTGGTTTTTCACTAATGCTGAGATTAAAGCTAAAATCGACTTATTGTTAAAAAATGTTAGTTCTTATGATTTTGAGGATTTAGATGATGAAGAAGAACATGATTATGAAATTGATGCATATGAAATTTATAAGGAACAAACTGATTTTCTTTCACAATTAGAAATTAATAAAAATATAGATGTGGACAATCCTAAAATTGCTGAAGGTATTTTATTAGATAAAGCATCACAAGAAGATATTAAAAAAACTTATCATTACATTAAAAAAATTATTAATTTCGAAACTGATCCTTTATTTAAAAACAAATCATTAGAAGAGCTTGCTAATTTAACTTTAGAATTAATTGATAAAAATGATAAAATCATTTTTTTTCAACCTGTTTTTATTAATGATAAATTAATTACTAAACCCGATTGTTTTATCAAAAAGAACGATGAATTTATTGTTATCGAAACAAAAGGGACAAGTTCAATTAAAAAACACCATATTTTAGATGTATTTTACCAAGCACAAGTTATCTCAAAACACCCTTATTTTATAAATAAATGTATAAGTTATCAAATGTGCATTGTTGATTATGTTAAATTAAAAAAAAATCAAGTAAATTTTACAATTTCTCCTTATTATAATTATCAAAAAAGCGTAGTTTTATCTTTACCACCAAGTGCTGCAAATCAATTTAATAAATTTGAAATTAATGAATTAAAACGTAAACGTAAGCGTGGAGAAGCAATTTATTTAAAAAAAGACGAAAATAAAAAATATTATGAAGATATTGATGCTTATGATCATTGTAAAGGTATTTTAATAGATGATTTAATCGATGGAACCTATAGTAGCATAAATGATTATAAGGAAGCCAAAAAAATTTATGATTACAAAACAGAAGATTTAAAAGATGATGTACGTTTTAATAAAACCATGGATGAATTAATTAAAGGCATTGAAAAATTACACAATGATTTTGATGAAGTAATCAGCGAATTATTAGTTCATAAACAAAATTTATTAGCTTTGCCTATCGAACAAAGAATTCCTAACTATTTTATACCATCATATAATGATAAGGGTGACTATAAGGATAATGATTTTTGATTAGAATTAAGAAATTTATACGCATATAGCGGATATGATGTTATTAAATATAGTGGAAAAATTTTAACAATTAAAAAATTTGGTTTAGATAATGTTACTAAAGATATATTAGGGATTGATATTTTAAAAACATATGCTAATCAAACCTTTTTTAATTTATATAAAAATAATCAAATAAAGATCTATCAAGCTGCTGTTGATTTATTTGCTAAATTGAAATCAAAAAAGGTTTATTTTGATTTTGAATCAATTAATTCAGCAATTCGCGCTGTTGATAATTCGTTTCCTTTTACCCAAACTGTTACACAAAATTCTATTATTATTGATGATGGAAGTTGTGATATTAAAAATTTAAAATGCACTAATATGATTATTGATCCACGTGAAATTAATAATGAATGGTTTAAAGCAATTATTGATAAAATTCACCAGGGATTAGAATATTCATATATTGTATACAACAAATCTTTTGAAGCTTCGCGTCTTAAAGAAATGGCTGAATTCATTAATGAAGAAATATATGAATTAAAGGTTAGCGAAATAATTAAAAACATGTATGATTTAGCTGATTTTTTTATAGTTTCAATGGGAAAGCAATTAATTGTAATTCCTGAATTAAAAGGTTTTTATTCAATTAAAAAAGTCTTACCAATTATTGAAAAAGAATTTCCACAAATTTATCACGATGTTAATTGTTTAAACTACAAAGAACTAAGTGTGGGTAATGGTTTGGTTTGTCAAACAAAAACAACAAAACGTTTTTTCAATACAATTACTGATCTAGAATGAGAACAATTTGTACATGACGCTCGTATTTATTGCGAAAATGATGTCCGTGCTATGATTGCTGTTGAATATTTTATTAAAAAACTTATTGATGAAGCTAAAATGAAAAATTTAATTTTAAGTTAATATAAAAAACTCTAGTGTTATTTTAAAGATACTAACTAGAGTTTTTTATATTAATTATTTTTTATTATTTGTTTCTGTAAGTGACAAAGGAATGTAATTTGTATTTAAATTTGTTTGTGTTTTTTCATCAATAGCAGTCATATTTTCTTTAATTTTTTTAATTAATTCGGCATTAGTTGTATTGTTTTTTATATCCAATGCTTTCAATAAATTGGGAATTAATTTATGGCTGAACATTACATTGTTAATATGATATTTTTGTTCTTTATCTAAATTAAATACCTTAGTAAACACTAATTTTGGTGATTTTAAAACTTCTGATTTTTTAAAGTTAATATATGAAAAGTTTTTAGGATTTTCTTTGCTAGTGTTTCAATCTTTTGGATATTCAGCAATTTCTAAACGCATTACTACAGGATCTTCAACTATTTTATCAGTTTCAATTGGCATTTTTGTTTTTTCATCTATTACTGGTTTACCATTACTATCTTTCTTATATTCTTTAAATACAACATCTTTTAATAATGTTGATCAGTCAAAGCCATTGTATAAATTAAATGTTGCAGTAACTGTTTTGTCTTTTATTACATCTTTATCTTCTTTAACAATACGGATTTTATTAGATTCAATTTCCACTGATGGCTCTAAATCATGCTCTTCAAACAAATTGCTTATTGAACCATAAAATTCATTAATAAAACTTGGATCATAATGAACTTTATCATTTACTTTTGTTGCTTTCATGATTGTTGTAAAGTAATCTTTAAATAATTCTAAAGCTTTATTTTTAGTTTCATTATTATCTTCTGCTAGCAATCAACGTAATCCTAAAATAATTTTTTCATAAATTTGTTTATCTTCTAAATTAAGGTCGTTGCTTTCTAATTTTTGTGCTAAAAGATCAATTTCGTCTAAAAACTTTTGAACAAAACTATGATTTTTATAATCTAGTGTTTTATTATTTGGATTTAAATATTGATACTCATTACTAATATATTTTTCTAAATTAGATAATGTAATTTGTTTTACATAATCTTCATCTTTAATTGCTTTTTCATAATCAACAGCTTTAGTTTGTTTTTCTTCTTTTGGTTCTAATTTTTGATCAGGAACTTGTTCAAAAGTTACTGTTTGATAATTTAAATTATCATTATTTAAAACTAATGCTTCTGTTTTTAAAATATTTTTAGTTGGTACAACAAGTAATTTTTCATTTACTTCTGGTTTGCGTGGCAATTTAACGTAAATCTTACCTTCTTTTTGAACTCCTTTAACTTTAATATTTATTGGATTTTTACCATCTTTATCCAAAATAGAAACACCAAACTCAAGATTTTCTAATGACTTCTTTTCTTCATCTGTTAGTTTAGTATAATTTTCAATTTCATAAACAGCAAAATGGTTTTTTTGATCTTTTGTTTGTGCTAATTGCTTGCTTAACTTAGATTTAATATTTGTATTTGAACAAGAAGCTGCGAACGCAACTATACTTGTACTAATTGCAATCGCACTTAATGACATTGCTCAAAATTTTTTGTTTTTTAATAATTTCATTTATATATAATCTCCTAATAGATTAATTTTTGTTATTTAAATTATTCTAATTGTAAAAATTTAAAAAGAACATTGTATATTGTACATAAAACAATTTTATTTATATAATTTATTTTAAGACACATAAAAACTGTAAATGCATTAGCACATTTACTAATTTATTTTACAATTTTATTTAATTATTGTCAAAACAATATTATATAAATTTATCATTTTTGGAGTTAACTACTTCTATATATATATATATATAAATTAAAGTTAATATAAATTATAATTTAAATTATCAAACAGTAACTTTTGAACAAGTTCCTAATCAAAACTCTACACCAGTACCAGAAAACCCATCAACACCAGAATCAACTAAAAAGGATGAAGTGATTGCAAATAAAATAACAATTAATAAAATTCAAAACGGCACTGCTACTATTGAAGTTACATTTAGTAAATTTAAATTAGCAGATGCTAATAAAAAAGATTTTGTTTTAGAAGTTAAAGATAGTGCTAATGATGACGCTTCTGCTATTAGTGCTACTGAATTAGTATTTGATGCAAATAAAAAAATACTTACAGGTAAATTAAATGGCTTAGCATCTAATATTAACTATAAAATATCAAAATTTACTTTAAATAATAATGAAATTAAATTTGATTTTAAACAAGAAGACCAACAAGAATTATTAAACCAATATATTCAACAAGCAGAATTAAATACAATAATTGATAAAGCTAATAAAAAAATTAACATTAAATTACAAAATTTTAGTATATTAAATTCTTTTCAAGATAATCAACCTGTATTAACATTTGATATTGAAATTAATAAAAAAGACGGAATAACACAAGTTGTCCATAAATCGTTAACAAAGAATCAATTATTAAATCCTAATGGTTTGGAAATTGATTTAAAAGATAAAATGAAAAATAATATTGATTATGATGTTAAATTGACTAATGTTAAAATTGCAAATATTGCATTAAATAATGATAATGTTAATAATATTTAACAAAATCATATTTCATTTCGACATCTATCAACCAATGATATTAAAAATTCTAATGATAAAGTAGAAGCTAAATTTTCAATTGGATTAGGTTTTGATATATCTGAAAAACTAAAAAATATTGCATTTAAAAAGTTAGACACTAATACTAATAAATTAATTGATATATCTGATCCAACAATTTTACGTTTAACTTTCTACGAATATAATAAAGATCAAAAAGTAGAAAACAATCTTGCTGCACCAACAGATAAAACAAAAATTTCAGTTGTTGAAATTAAAAAATCACAATTAATGAATTCATCTATTGTTTCTTTTGTAAAAAAATTAAACAAAAATGCTAAGTATCATATGGCTAATATCGCCTTTAACCAACAATTATTACCATATTTAAATAAACAATTCAATGTTAATGCTGATTTTCCAATTGATCTAGAGCAAAATCCAATCGACTTTAATGATAATAAACAAGCCAATGATGAAAAAATTAAAAAGACAAAAGAATCTGCTATAAAAGTAGCTACAGCAATTGCTAAACAAATTGTTGGAATAGATTATAAAGGAAGAGACATTATTAAAAATTTTATTGAATTAGATAATGTAGTAAATAAAAAATAAAAACCAAAAAACCTGTAATTATTAATTAATTATGGGTTTTTAATTTTATTTAGAGAAGAAAAAACCAAACCGTTTTGGTTTGGTTTTTATAATTATAATGGTGGAGACGATGGGGGTCAAACCCACGACCTCCTGAGTGCAAATCAGGTGCTCTATCAATTGAGCTACGTCCCCAAATGGTGGAAATAAGTGGGCTCGAACCACCGACCTCACCCTTATCAGGGGTGTGCTCTAACCAACTGAGCTATACTTCCAATGTACCATATTATTATACAACAGACAAATTTAAAAAGCAATATTTTTTAAAAATATTTTTAATTACACAAAATATTTATTCTCTATATAGAATGATAAACATTATTTTTACTACAATATTTTTAATTATGGATGCAATTAAATATAATAGTAAAATATTTTAAAATATTTCAGATAAAATAAAAATATGAAAAGAATAAAAAACAGAAAATGATGAAAATTCGGTGGAGTATTATTTTTGCTCCCCCTTGCTTCTTTTTTAATAATGTGCAAGAACCAAGAAGCACTAAAGACAAACCAAGAAGCACCAAAAACAAACCAAGAAGCACCAAAAACAAATAAAGAAAATGTCTTAGACGATTTTGATGCAAATATAATTAATATAGAAAATATTAGTAAACCTAAATTTATTAATCAAAATAAAACAATTATTAATTTAAAATTAAAAAAATTTAATTTTAAATCACCAAATACTACTATAACCTTAACTTATAAAGATAATAATGGTCACAAATTTACATCAGATCCTTTAAGTATTAATGATGAACAAAATTATGATTTTATTTTTTCTAATTTAACCCCTAATCGTAAATATCAAATTCAAAATTTAACTTTTAATAATCAAAAAAGAACAGACATTTATTTGAAAACCAACCTAAATAATGCTATTTTTTCAATAAAACCAATACCTATTAAAACCAATAATTTTAAAATAAAAGTTTTTAATCAAAATGCATTAATATCTTTTAGCATTCCTAAAAATTCTGATGTAAGAGTAAATGAAAAAATAGCATTAGAATTTGAAAATTTAAGTAGTAATTTAGTGCCTAATAACGAAATAATTAGTAGAATTGATAAAAATTTTAATGTAGAATTTAAATTAGATAATTTAAAATTAAATAATAAATATCGAATTGTAAATCTTAGATTTTTAGATACTAATCCACCTAATGTTAGTCCAAATATTTTTGAAAAGTTATCTAATTATGAAAGCAGTTTTATCATACCAGGTATTAAAACAAATATGCATAATCATAAAGACTTGAATAGTAATGACAAAAAATATATTGATAGTCCTTATAACACTAAAATTGAACTTAATGATAAAAATAATTTTAATGATAAAGTGTTGCCAGATGCATTTGAACAGCAAAACATTAATATACAAGAAGTTGAGAATCTTAATCAAGACATCTTAAATCAAAATGAGCTAGAATTACAAAAATATTTTAGTTTTATTAGTGATCAAGCAAACCTTAAAGACAAAGATTTTCAAAACTATTCTTTTAATGATATTAATAAAAATTCAAAGCAAGAAATTAAATTTAAAATCAAACATATTAACATCGATAGTAGTAATAAAAAAGCAATCATTGAATTAGATTCGTCTAGTTCTAATGATAATACTAAACTTTTAGAAGCAAATAATAAACAATTGTTAATTAAATCATATGATTATAATAATCCCTGATCTAAAATTGTTAGTTATGAAAAAAAAGATAATAATAAAATGATATTTGATTTACATGATTTTCCAAAAGATTTGAAAACATTTATTATTACTCATATTCGTTTTGATGATAATATAACTTCGCTTGGTAAAATTAAAGAAAATAGTTTTGAATACTATCAAAACGACAAGGAATATTTACTAAAATCACTTAAATACTACTTTGATATAAAAGAAAATCAACTATACGGTTCTGCTTGTTTTAATTTTAATAATGATGATTTTAAAATCCTAAAAAATAAAACTTTTGTTTTTAAATATGAAATCGACACTAAAAATAATATTTTAAATAAATATATACCATTAAATAAATATATAAATGTTGATTTTAAAAATCTAGCACAATTTAAAATTGTTAATGTTTTCGATGGATTAAATTATAAACTTGAAAGTATAAAAATAGTTAATAAAAACAGCTTGTTACCTTATAATGATCATGTTAATATTCAAAATGCTAATAATACAAATTTTAGTGTTTGACATAAAGTTTATCCAAAGCAAAATATTATTAATGAGTTTTTTGATGATTCATTAATAAAAGATGAATTTAATTTATCAAAAATAGATTTTAATCACTTATGAAAAAATAATACTGATCAAAAAAATATCCCTTATTCTTTGCGCAATTTAATATCATTAACTTTGCATGAAAGAGAATATGCAATTTATAAGCATAATGCTACTAATGGTTTTTATTTATATAACACAATAACAAGTAATAAAGATTTTAATTTAATTAAAAATAATAAAGAGTCATATCATCTAAATTCTTTAATTGCACATTTAGCAATTAAAGATGAAGGATTAAAAAAGGATGAATCTGCTGGTTTCTTCTTAGAAAAAGATTTGAATGATTTTGATAATTTAAATAATTTTAAAGATGAAGATATTGTCTTTAATGTAGATCTAGAATTAGATCCTAATCTAATTTATGAATCACAACTTGTAGATAAAAACATGCGAAGATCGCATGTGATAATTCCTATTTCATATAAAGTAATTAAAAAACAACACATTTTAGAAGATGTTGAATTTAGTTTAAATTATGCTTTAGGTTCTGAAGCTTATGAAAATCACATCTATCAACAAATTAAATCACAATTAAAATTTAATGTCTTTTTAAATGGTTCAAAGATAAAAGTTGAAGTTAAGCCACGCAATGATAATATTAAGCTTTATGATTACGTTTGAAAACACAATAATTCAAATCAACCGTCATATTTTATTGGAAGATATGATTTTATTGTTAATTGATTAACAAATAATAATGAAGTTATTATCGATAAAAAATTAGAGGAATTTAAAAAGAAATCTTATACCGCAAGAATTTTAAAAGACAATGAAAATGAAATGTCAAAAGCGGCAATAAAACAAGTTCGTGAGCGTACGATTACTTTTAGTTTAACAAGTGATGGTACTTGAAATTTTTTAGGTAAGGTAAAACCAAATGATCCAAATGATTATCGTTATTATATGTTAACTGATCATCATGTTATTGGTAGTGGTGGTAGTTGATATAATCCCCAAATAAATTGGGCTGGAAAAATGGAATATGAAAGTATTAATTTAAATAAAAAAACTATCTACGATGATGATGGAAATGAAAAATACACATATTCGAATTTTGCAGATTATACAGTTGTAATTCCTCAAACAATTAGTCAAAATGATTTAAATAAAGATAAATATCAACCATATTATAATTATAGTAGTGTTAATAACCAAGATACTCCAGGAAAAAATCAACTTCAATATTTTGCTTTTCCTTTTAAATTAAAATTTGAAAAAGTAATGGATTTTTGTTTACAAAAAAATAATATATATAAACACTACAATGCTTTAGGTAGATATGATGATAAAATAAGTGAATTAGATTGGTCAGTAGTTAGTATCGATCTAAAACCAATTTTTGAAGCTTTTAAAAATCAAGATTTAAATAAACCGTTTATTTACAATAATAAAACTTTGTCTCCAGAAGAAACTAGTGTTATAAAATATTTTTTAAGTTTAAAAAATATAAAACCATTAGAGGTTAGTCCACAAACAAGATATGTTAGAAGCAACCAAGATGTTGATTGGTATATTGGCACTTTCCCTCGTTATACAAATACTAACCAAAATTCAATGGGGGTTGGCGAACTACGTTATCGTGAATATAATATACAAAAAATCGATTCTGTAAATACTAATTTTGTCACAGGTGGTAAGGGCGTTTTATATAAATCAGATATACCATATACAACAATTAGTACTGACTATATAGATGCTGCGGGTGGTTCATCTGGAACTAGTTTATATGATGAACAAGGACGATTCGTTGGATCAATTGCAACTGGTAGAACTCCAAGTAAAAATGGTCATCCAACGTGAGAAACTATAGGTTGAAGTTTAATAGATAGTCAAATTAGTGGGTTTTTTGGTGATCGGGAAAATAGAGCTAACAATAGTTCGATTATCCAACAAATTAAACAATTAGCTTATTTATATCCAGAAAAATATGAGGATATCTATAAGTAATATAAATCCTTTTAAATAAGATTTATGTACATTCAAAATTAAAAAAAGAAAGAAACGTTGTTAATTTAATAACCGTTTCTTTCTTTTTATTTTAATAAGAAATTTATATATCTTTTAATTTTTTAACTAATTCAGAATCGTTGATTAATGTATATAAATCATTACGCTTTTTGTTTTTTTCATAAATTAAAAGTGCTTGTTCATATTTTAATAATTGGTCACACACTTTGTTTAAACAATCACTAATAGCGCTTTTTGAAATTTCTTTTAGTTCAGCAATTTCAGAAAACGACAAATCTTTAAAATAATGTAAATTAAAATATTCACATTGTTTTGTTGTTAATAATCCTTGATAAATATCATATAAAGCAATCAAATATCAACGTTTATTCTTATTTAACATCTAATTTCATTCCTAAAACTATGCTATCAACATACATTTCTAAATCAAATACACTTAAATCCTCTAATTTTTCGCCTAAACCAATCAATTTCACAGGTATATTAAAAGCATCTTTGATTGCTAATACAATTCCCCCACGACTTGTAGAATCCATTTTAGTTAAAATAATCCCTGAAATTTTCGTTACTTCGTTAAATGCTTTTGCTTGATTAATTCCTGATTGGCCTTGAGTGGCATCTAAAACTAATAAAGTTTCGTGCGGAGCGTATTCATCAAATTTATGAATTACATCATGAATTTTTTTTAATTCATTCATCAAATTAATTTTATTTTGTAATCTACCTGATGTATCACAAATTACTAAATCATATTTTTGTTCATAACCTTTTTTAACTCCAGCGTATATGACAGAAGCAGGATCTTGACCCTCTTTATTAGGTAATTCAATGTCAACACCTAAGCGTTGTGCTCAAACTTTTAATTGTTCAACAGCACCTGCTCTAAATGTATCTCCAGCTACTAAGAGAACTTTTTTATTTTCATCAATGAATTTTTTAGTGATTTTTGCGATTGATGTTGTTTTACCAACGCCATTAACCCCAACCACCAAAACTACATTAGTTTGATTTTCTTTTAAATTAATTGCTGTATTAATTTCTGTATCTTGAATATAATAAATAAAAATTTTATCAATAATAATAGACTTTATTAAATCAGGATCATTAACTTTTTGGTAAATCATTTCATTTCTAATTGATTCAATAATTTTATTAGTTGCAACATAGCCTACATCATAACTAATTAAAACTTCTTCTAAATGTTCATATCATCGTTCATCAATATCTATATATTTAACTGCTAATTCGTTAATAGCATCAGTTAAAGCAGAAGATGATTTTCTTAATCCTTCATTAAATTTATTAATCGAATTATCCTCTAATTTTAAAATACGATTTTCTTCATTTTTTTGTGAAATCTCTTGTGATATTTGTTCTGTGTCTTTTTTTCCTAGAATTTTATTGAATATTTTTTTAAAAAAACCCATTTTTATAATTCACTTTCATATTTTATTTTTGTATCATTTAAATCAACTTCTAGTAATGTTGTTACTCCGTTATTCATCATTGTTGTACCTAAAAGATAACCACAACTTTTCATTGTTCCTTCGCGGTGAGTAATAACTAAAAATTGTGTTTCATCACTATACTTAGCGATAATGGCACCGAATTTTGCAACATTACTTGCATCTAAAGCTGCTTCAGCTTCATCTAAAATAACTAATGGAAATTTTGCAATTTTTAATAAAGCAAATAAAACTGCCAATACTACCATTGTTTTTTCCCCACCTGAAAGTGCAGATAATGGAACATCACGTTTACCTGGTGGATTAGCAATTATCTCAACACCTGATTCTAAAATATTATTAGGATCATTAAGACTAATTGCACAAGATCCTCCACCAAATAAATATTCAAAAATCTCTGGCATTAATTTATTTGTTTCACGAATAACACGCATAAAATCAGCTTTAGCTTTTTTATCTAATTCTTGAATTGAACTACTAATTTGATCAACCGCTTGCTTGCAATTTTCATACTCTAAAAAATTAGAATCATATTCTGCTTGTTTTTCTTCGTATTCCTCTAACGCATTTAAATTAATATCATTATATTTTTCAAGGTCAATTTTTAAACTTTCGATTCTTAAACGTGCTGCTGATTCACTCATATCAATTTCTTGATTTTGATATGTTTCAATAGCATTCTCAACACTTAATTTATAATTATCTACTAATCTTGTTTGTACATTTTGAATCGAATAATTTAGATTATCTATATTACTTTCACACGAAGTAATATTTTTATCAAGATTATAAAAATCTTCAAGCTCCTTATTATATGTTATTTCTAATTCATTCTTATTAAGATTAGCAGTGTTTAAATTTGCTTTTAAAGTTTGGATTTTTTGTTTTAAATTAATTAATTCATTCTTTTTTTTATTATATTCATTTCAATTATCACTTATCTTTGATTCAATCTCTTCTTTAGACAATTTTGTAGCGCTTAAAGCCTCATATTGACTTTTATAATTATTTAAATTATCAGAAAGACTATTGTTAGTAGTAATTAAATTATTTAAAATACCATTTATAATTTGTTCACTATTATAATATTCTTGTCATTGCTTTTTTGTTTGGTTATGTAAATTATTTAATTCAATTTGTTCTTCATTTATTTTTTTTATTTTTAAATCTAATTCTTTAATTTTTTCTTCTAGATTTAAAATAATATTTTTTTTACTATTACTTCCACCAGAAATGGCACCACCACCAAAAACAATGTCACCTTCTAATGAGACTACTTTATAATTTTTATATGTGTATTCACTTAGAATGATAGCGTTTTTTAAATTATTAGCAATAATTGTATTTCCTAATAGATAATCAATAGAAATTTTATAATTTACATTAATTTTTACTAGTTCATTAGCAACTCCTACAAACCCCTCTAATTCTTTTAATACATCATAAAATTCTGGTTTAATGCTTTTAGCCTGCAAATTTTCAATCGGTAAAAAAGTAGCAACACCTGCATTATTTTTCTTTAAAAAATTAATACAATTTTCAGCTGCTTGATTGTTTTTAACAATAATATTATTGATTCCTTTAGCTAAAGCTGTACTTATTGCTAATTCGTGTTCTGTTTTTGTAGAAATATTCTGCGCAACTGTTCCATAAACACCAACTAATGCTTTTTGTGAATCAATAATTGTTTTTACACCACGTTCATTCTCAGTTTGGCGTTTAAATTGCTCTTCTAACATTTCTTTTTTTAAATTCTTACTAGCAATTTCTCAAATGATGGTATCCAAATTATTTTTTTGTTTTGTTTTAAGAGATTCAAGTTCTAATTTTTGTTTTTGAATATCTTTAAGTTGATCTTTTTTTTCATTAATATTTTTTTTATTATTATTTAATTCTATTTCCGTTGTTTTAATTAAAGTAGCAAAAGCATCAATCCTTTCTTGTGTATTTGTTGCATTAAAAGCGGTTGTTTCAAGTGTATTTTTAGTTAGAGATAATTTAAGATTCAAATCGTTTATTGTATCTTCTAATTTTTTATATTCAAATTCTAAATTAGTGTATTCTTTTGCATATCTATCTGAAATTACTCGTAAACTTTCAATTTCATTTTTTTTACTTTGAATTAATGGTTTTTGTTTATTATATTCTTTTTTATATTCTTTTAAATTTTCTTGAATATCATTCAATTGTTTTTGTCAGTGAATGATATCACGTACTATAATCACTAAATCTAATTTTGTTAATTCGTCCTTAATTTGTTTATATTCATTAAAACGCATTGCTTGCTTTTGTAATTTCGCCAATTCTTTTTTTAAATTAGCAACTATGTCATTTAAACGAGCTAAATTTTCGTTTGCACGACTTAGTGAATTTAACGCTTCTTGCTTTCGCTTTGAATAGCGCCCGATTCCAGAGGCCTCTTCAAACATTTTTCGTCGTTCATTCGGTTTTGCTTCAGCAAATCATGACACTGCACCTTGACTAATAATTCCTAAAGAACCTTTTGATAATCCACTATCTAAAAACATATCTGTTATATCTTTTAAAAGAGCAACTTCATCATTAATATAATAAGTATTTTCGTTTGTTTTAATATTCAACACTCGTGAAATTTTAATTTCTTTATGTTGTGTATATAAAACATTATTAACATTATTAAAATATAAATTAACACGTGCAATATGGGCTTTCGGTGCATATCTTCCACCACTAAACAATAAATCATTTTTATGAGCACGCATGCTTTTTAATGATTGATCACCAATTACTCATTTAAGAGCATCAATAATGTTTGATTTTCCTGTTCCATTTGCACCAATAATTCCAGTCATTGGGTGTTTAAATTCAATAACAACTGGCTCCCCAAATGATTTAAACCCATGTGCTTCTATTTTCTTTAAAAAAATCATATAATCTCCTAATTTAAATTTTAAGTTAATAAGATTTTATTTATATTTTATATATTATAATTTATTCAATATTGATCACTAAATATTTATGATCTCTATCAATTATTTTGTATGTATCATATTTTCTCATGATTTCTTCTAAAAATTCATTATTTAAGTTATCGCCTAAATTTAAAACAGATTTTAGAATTTCATCTTGAACACGATAAGCTTTTAAAAAATTCTGTGATTTAGACTGTACAACAAAATCCACAAAACCATAATTATGTAATAAATTGGTACTAATTACACCTTTAGATTCATCTTTTTTAATTTTAAAAACATATTTTTTTCAATTTGGATCAATTAAATATGAATTGTTAAAATTATAAGTAATAAAATAAATTCAATTAACTACACTCATTTGAATCGTTCAAAAATTAATGTGTCTGATAGAATTTTGATTTTTTTCAATGATATAATTTACTAAATCAAATAAAATTAAACTATTCCTTTCGTTATAAATATTTTTATTTTCATTAATATTAACAATTTGATACGAATGAAATTTATTGATATCTAAATTTGTTATAGAAATTAATTTATTATCAATTTCTAAAATAATTTCATTTGTATTATTTTTGATAATTTTACCTTTTTTATATTGTTTTAAAATTTGATTTATAATCAAAAAAACATCCGTTTTAAATTCTAATATTATTGGTTGAGTCACATATGATTTTGAATTTGTTAGATTGTTTTCTAATAATTGAATAAAAATTTGTTCATCCATACCTTCTTGTATTCCTTATCTTTTGTTTTAATAATTCATTTAATATTTATTAGTGATTATTCTTTAATAAAAAGCACTAATTTATTATAGTTATAATACAAACTCGTTTTTTTAAGTAATAACGTGTAATTAAAATATATAAAAACATCATTTTTTATTTATAATATAAGCAAGCCAACT", "length": 727289, "complete": true, "type": "contig", "topology": "circular", "simple_id": "contig_1", "orig_id": "GCA_000828735.1|AP014584.1", "orig_description": "HMT-799 Ureaplasma parvum SV3F4" } ], "run": { "start": "2026-01-26 13:21:37", "end": "2026-01-26 13:29:34", "duration": "7.95 min" }, "version": { "bakta": "1.11.4", "db": { "version": "6.0", "type": "full" } } }