{ "genome": { "genus": "Mycoplasmoides", "species": "pneumoniae", "strain": "S63-tet-R", "taxon": "Mycoplasmoides pneumoniae S63-tet-R", "complete": true, "gram": "?", "translation_table": 11 }, "stats": { "size": 816435, "gc": 0.40009553730548053, "n_ratio": 0.0, "n50": 816435, "n90": 816435, "coding_ratio": 0.795960486750323 }, "features": [ { "type": "oriC", "sequence": "contig_1", "start": 29, "stop": 691, "strand": "?", "product": "origin of replication", "nt": "GGTATTAAGATGCTACCAACGTGGTATTAAAATGTGCCCAACCGCGAAAAAGAAAGTGGTATATAGGAAAATGGAAAACAAATTTGTTTGCCGTTTACTATTAAATACTAATCTTCTATATAGTATAGAGAAACTTTTTCTTTAACATAATATTATCTTAATATTATTTACCTACTAATAGCTTAATATTATTAGTATTTATTTAGTATTATGCTAATACTATGCAGATATTATCTTAATATTATCTATAGTATTAGGCTAATATTATTCTTAATATTTATTAAGGTACTAAAGCATTACCTATAGGTGATATTATGACAATACTAAAGTGGTTAGTATTATTAGGGTATTATTCAAAGTATTCTCCAACACTATTCCCTTAGCTCTAACTTAATATCCCTTTAATACCAAGATGGTTAAACAATGAAGAAAATTAGTTGTTTGTTCTGGCCCTTTACTATATATGTACATAGTACATATATAAGAAAGGCTACCATGACATTATATATAATAGGCATCCAGAAATAAAAGCCAAATTAATCTATAATTAAGTATTTATACTTAATTATAGTTATTTTATTAACAAAATTAGTGACATTTGGCCATCCATAATAAATAAAATCAAATAAACAAAACAAAAAGCAAAAAAACTATAAAAAGGGC", "id": "APKECPOBEN_1438" }, { "type": "cds", "sequence": "contig_1", "start": 692, "stop": 1834, "strand": "+", "frame": 2, "gene": "dnaN", "product": "DNA polymerase III subunit beta", "db_xrefs": [ "COG:COG0592", "COG:L", "EC:2.7.7.7", "GO:0003677", "GO:0003887", "GO:0005737", "GO:0006260", "GO:0006271", "GO:0008408", "GO:0009360", "KEGG:K02338", "RefSeq:WP_010874358.1", "SO:0001217", "UniParc:UPI0000129701", "UniRef:UniRef100_Q50313", "UniRef:UniRef50_P47247", "UniRef:UniRef90_Q50313" ], "nt": "ATGAAAGTTTTGATTAATAAGAATGAGTTGAACAAAATCCTCAAAAAACTCAACAATGTAATCGTATCTAACAATAAGATGAAACCATACCACTCTTATTTATTAATAGAGGCTACAGAAAAGGAAATTAACTTCTATGCTAACAACGAGTACTTTTCTGCTAAATGTACCTTAGCCGAAAACATTGATGTACTTGAAGAAGGTGAAGTAATTGTTAAAGGCAAAATCTTTAGCGAACTCATTAACGGAATTAAAGAAGACATCATTACTATTCAAGAGAAAGATCAAACTCTTTTAGTCAAAACAAAAAAAACAAACATTAACCTTAACACGATTGATAAGAAAGAATTCCCCAGAATCCGTTTCAACCAAAACGTTGATTTGAAGGAATTTGATGAACTTAAAATCCAACACAGCCTTTTAACTAAAGGACTTAAAAAGATTGCCCATGCTGTTTCTACATTTAGAGAATCCACTAGAAAATTCAACGGGGTTAACTTCAACGGTTCCAATGGTAAACAAATCTTTTTAGAGGCATCGGATTCTTATAAGCTCTCTGTTTATGAAATCAAACAAAAAACCGATCCATTTAATTTCATTGTCGAAACTAATCTTTTGAGCTTCATCAATTCTTTTAACCCTGAAGGTGGTGATTTAATCAGTATCTTCTTCCGCAAAGAACACAAGGATGATTTAAGTACCGAATTACTGATTAAGTTAGATAACTTCTTAATTAACTACACCTCAATTAACGAAAGCTTTCCGCGGGTAATGCAGTTGTTTGACTTTGAACCAGAAACCAAAGTAACCATTCAAAAAAACGAACTTAAAGATGCATTACAAAGAATCTTGACGTTAGCTCAAAACGAGCGCTTCTTTCTCTGTGACATGCAAGTAACCAACTCCCACCTCAAAATTAATTCCAACGTTCAAAACATTGGTGCATCCTTAGAAGAAGTTACTTGCCTTAAGTTCGAAGGTCACAAACTCAACATCGCTGTTAATGCGCTTTCCCTCTTGGAACACATTGACTCATTTGATACTGATGAAATTGAGCTTTACTTCCAGGGCAGTAACAAATACTTTTTAATTAGTTCGAACAACGAACCTGAACTTAAAGAAATCCTAGTTCCTTCCAAGTAA", "aa": "MKVLINKNELNKILKKLNNVIVSNNKMKPYHSYLLIEATEKEINFYANNEYFSAKCTLAENIDVLEEGEVIVKGKIFSELINGIKEDIITIQEKDQTLLVKTKKTNINLNTIDKKEFPRIRFNQNVDLKEFDELKIQHSLLTKGLKKIAHAVSTFRESTRKFNGVNFNGSNGKQIFLEASDSYKLSVYEIKQKTDPFNFIVETNLLSFINSFNPEGGDLISIFFRKEHKDDLSTELLIKLDNFLINYTSINESFPRVMQLFDFEPETKVTIQKNELKDALQRILTLAQNERFFLCDMQVTNSHLKINSNVQNIGASLEEVTCLKFEGHKLNIAVNALSLLEHIDSFDTDEIELYFQGSNKYFLISSNNEPELKEILVPSK", "aa_hexdigest": "b3c4e88f68473c2c7f1854ed094678a7", "start_type": "ATG", "rbs_motif": "AAAA", "ups": { "uniparc_id": "UPI0000129701", "ncbi_nrp_id": "WP_010874358.1", "uniref100_id": "UniRef100_Q50313", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000129701", "RefSeq:WP_010874358.1", "UniRef:UniRef100_Q50313" ] }, "ips": { "uniref100_id": "UniRef100_Q50313", "uniref90_id": "UniRef90_Q50313" }, "psc": { "uniref90_id": "UniRef90_Q50313", "gene": "dnaN", "product": "DNA polymerase III subunit beta", "ec_ids": [ "2.7.7.7" ], "uniref50_id": "UniRef50_P47247", "cog_id": "COG0592", "cog_category": "L", "kegg_orthology_id": "K02338", "go_ids": [ "GO:0003677", "GO:0003887", "GO:0005737", "GO:0006260", "GO:0006271", "GO:0008408", "GO:0009360" ] }, "pscc": { "uniref50_id": "UniRef50_P47247", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47247" ], "product": "Beta sliding clamp" }, "genes": [ "dnaN" ], "id": "APKECPOBEN_44", "locus": "GCA002090215_00001" }, { "type": "cds", "sequence": "contig_1", "start": 1838, "stop": 2083, "strand": "+", "frame": 2, "gene": null, "product": "Chaperone protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W8W2", "UniRef:UniRef90_A0AB38W8W2" ], "nt": "ATGACGCTTTATGACTTACTAGAGCTGCCCCAAACTGCTACACTACAGGAAATCAAAACAGCTTACAAACGCTTAGCTAAACGTTATCACCCTGACATCAATAAACAAGGGGCAGATACCTTTGTCAAGATTAATAACGCCTATGCCGTTTTAAGCGATACCACCCAAAAAGCGGAGTATGACGCTATGCTGCGCTTTAGTGAATTTGAAGACCGAGTTAAACGCCTGGACTTCTCGATTAAGTGA", "aa": "MTLYDLLELPQTATLQEIKTAYKRLAKRYHPDINKQGADTFVKINNAYAVLSDTTQKAEYDAMLRFSEFEDRVKRLDFSIK", "aa_hexdigest": "ada1fc6b9036bcb5b7b7243b6261452f", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 1838, "stop": 2306, "observations": { "insertion": [ 2240 ], "mutation": [ 2081 ], "stop": [ 2081 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB38W8W2", "query_cov": 1.0, "subject_cov": 0.5225806451612903, "identity": 1.0, "score": 161.0, "evalue": 1.79e-49, "gene_start": 1, "gene_end": 81, "reference_start": 1, "reference_end": 81, "reference_sequence": "MTLYDLLELPQTATLQEIKTAYKRLAKRYHPDINKQGADTFVKINNAYAVLSDTTQKAEYDAMLRFSEFEDRVKRLDFSIKWHEQFMEELQFHHNWDFDFIRNREYTQPTPTNNKYSSFLDKDVSLPFYQLYSKASWTLTWKTHFLDVIRSSKPS" }, "paralog": false, "identity": 0.9870967741935484, "score": 309.0, "evalue": 5.68e-110, "description": "Internal stop codon at 2081. Frameshift due to insertion around 2240. Nonsense mutation around 2081.", "psc": { "uniref90_id": "UniRef90_A0AB38W8W2", "product": "Chaperone protein", "uniref50_id": "UniRef50_A0AB38W8W2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB38W8W2", "UniRef:UniRef50_A0AB38W8W2" ] }, "pscc": { "uniref50_id": "UniRef50_A0AB38W8W2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W8W2" ], "product": "Chaperone protein" } }, "id": "APKECPOBEN_45", "locus": "GCA002090215_00002" }, { "type": "cds", "sequence": "contig_1", "start": 2096, "stop": 2359, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAGGAATTACAGTTCCACCACAACTGGGACTTTGACTTTATCCGCAACAGGGAATACACCCAGCCAACCCCTACCAACAATAAGTATTCTTCCTTTTTAGACAAGGATGTATCCTTGGCCTTTTACCAACTTTACTCCAAGGGCAAGTTGGACTTTGACTTGGAAGACACACTTCTTAGACGTCATTCGATCAAGCAAGCCTTCTTAAAGGGCAAGAAGCTTAACGATGTTTTAAAAGAGCAGTACAACTATTTAGGTTGA", "aa": "MEELQFHHNWDFDFIRNREYTQPTPTNNKYSSFLDKDVSLAFYQLYSKGKLDFDLEDTLLRRHSIKQAFLKGKKLNDVLKEQYNYLG", "aa_hexdigest": "1884f69e138bdc17e4d04b1d4c8b15b0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10555.729799999992, "isoelectric_point": 6.824566841125488 }, "id": "APKECPOBEN_46", "locus": "GCA002090215_00003" }, { "type": "cds", "sequence": "contig_1", "start": 2417, "stop": 2530, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCGTGAAGGTGGGGTGGTCAACCTCCCTTTAAAGATCAAAGTCATTTCCAATAACTACCCTGGACAAATGTGGTACGAACTCAACAAGAACTATTCCTTCCGGCTTCTTTGA", "aa": "MREGGVVNLPLKIKVISNNYPGQMWYELNKNYSFRLL", "aa_hexdigest": "a612c54cf5a52c79fd9448aaf9c7bdd7", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4386.1042, "isoelectric_point": 9.696383476257324 }, "id": "APKECPOBEN_47", "locus": "GCA002090215_00004" }, { "type": "cds", "sequence": "contig_1", "start": 2869, "stop": 4821, "strand": "+", "frame": 1, "gene": "gyrB", "product": "DNA topoisomerase (ATP-hydrolyzing) subunit B", "db_xrefs": [ "COG:COG0187", "COG:L", "EC:5.6.2.2", "GO:0003677", "GO:0003918", "GO:0005524", "GO:0005694", "GO:0005737", "GO:0006261", "GO:0006265", "GO:0034335", "GO:0046872", "RefSeq:WP_010874360.1", "SO:0001217", "UniParc:UPI000012BECC", "UniRef:UniRef100_P22447", "UniRef:UniRef50_P22447", "UniRef:UniRef90_P22447" ], "nt": "ATGGAAGACAATAACAAAACGCAAGCTTACGATTCCAGTAGCATTAAGATTCTTGAAGGCTTAGAGGCCGTGCGTAAGCGCCCCGGCATGTACATCGGTTCCACCGGCGAAGAAGGGTTGCACCACATGATCTGGGAAATCATTGATAACTCAATTGACGAGGCCATGGGTGGCTTTGCGAGTACTGTCAAACTAACCCTTAAAGACAACTTTGTCACCATTGTGGAGGATGATGGCCGGGGCATTCCCGTTGACATCCATCCTAAGACCAACCGCTCCACCGTTGAAACAGTGTTTACGGTACTCCACGCTGGGGGTAAGTTTGACAACGATAGCTATAAGGTGTCAGGGGGACTCCACGGTGTGGGTGCCTCGGTTGTTAATGCTTTAAGTTCCTCCTTTAAGGTATGGGTAGCCAGGGAGCACCAACAGTACTTCCTAGCGTTCCACAACGGTGGGGAAGTGATTGGTGATCTTGTCAACGAAGGTAAGTGTGACAAGGAACACGGTACCAAGGTTGAGTTTGTCCCGGACTTTACCGTGATGGAAAAGAGTGATTACAAACAAACGGTAATTGCTAGTAGGTTGCAACAATTAGCCTTTCTTAACAAAGGGATCCAAATTGACTTTGTTGATGAACGCCGTCAAAATCCGCAAAGCTTTTCTTGGAAGTATGATGGTGGCTTAGTCCAATACATCCACCACCTCAACAACGAAAAGGAACCTTTATTTGAGGACATTATCTTTGGTGAAAAAACCGATACTGTTAAATCAGTTAGCCGTGATGAGAGCTACACAATTAAGGTGGAAGTGGCCTTTCAGTACAACAAGACGTATAACCAATCAATCTTTAGTTTTTGTAACAACATTAATACCACTGAAGGCGGTACCCATGTTGAAGGCTTTCGCAATGCCTTAGTGAAGATCATTAACCGCTTTGCTGTCGAAAACAAGTTCTTAAAGGAAACGGATGAAAAGATTACCCGCGATGACATCTGTGAAGGGTTAACGGCCATTATCTCAATTAAGCACCCCAACCCCCAGTATGAGGGTCAAACCAAAAAGAAATTGGGTAACACCGAAGTGCGTCCTCTAGTTAACAGTATTGTTAGTGAAATCTTTGAGCGCTTTATGTTGGAAAACCCCCAGGAAGCCAACGCCATTATTCGCAAAACGCTGTTAGCGCAAGAAGCACGGCGCCGTAGTCAGGAAGCACGGGAACTGACCCGCAGGAAATCTCCCTTTGACAGTGGTTCACTCCCGGGTAAGTTAGCAGACTGTACCACCCGTGATCCCTCAATTAGTGAACTGTACATTGTCGAGGGAGATAGCGCTGGTGGTACGGCTAAAACGGGGCGGGACCGTTACTTCCAAGCGATCCTACCATTAAGGGGTAAGATCCTCAATGTGGAAAAGTCCCACTTTGAACAAATCTTTAATAATGTGGAAATCTCGGCTTTAGTCATGGCCGTTGGCTGTGGCATTAAACCAGACTTCGAGTTGGAAAAACTGCGGTATAACAAGATCATTATCATGACCGATGCTGATGTCGATGGGGCGCACATCCGTACCCTCCTTTTAACCTTCTTCTTTCGCTTTATGTATCCCTTGGTGGAACAGGGCAACATTTACATTGCCCAACCCCCACTGTATAAGGTCTCTTACTCTAATAAGGACTTATACATGCAAACCGATGTCCAACTAGAGGAGTGGAAGCAACAACACCCTAATCTGAAATACAACTTACAACGCTACAAGGGTTTAGGGGAAATGGATGCCATCCAACTGTGGGAAACCACGATGGATCCCAAGGTGCGTACCTTACTAAAGGTAACCGTTGAAGATGCTTCAATTGCTGATAAAGCCTTCTCCTTGTTAATGGGCGATGAAGTTCCCCCAAGACGGGAATTCATCGAACAAAACGCACGTAACGTAAAGAACATTGATATCTAA", "aa": "MEDNNKTQAYDSSSIKILEGLEAVRKRPGMYIGSTGEEGLHHMIWEIIDNSIDEAMGGFASTVKLTLKDNFVTIVEDDGRGIPVDIHPKTNRSTVETVFTVLHAGGKFDNDSYKVSGGLHGVGASVVNALSSSFKVWVAREHQQYFLAFHNGGEVIGDLVNEGKCDKEHGTKVEFVPDFTVMEKSDYKQTVIASRLQQLAFLNKGIQIDFVDERRQNPQSFSWKYDGGLVQYIHHLNNEKEPLFEDIIFGEKTDTVKSVSRDESYTIKVEVAFQYNKTYNQSIFSFCNNINTTEGGTHVEGFRNALVKIINRFAVENKFLKETDEKITRDDICEGLTAIISIKHPNPQYEGQTKKKLGNTEVRPLVNSIVSEIFERFMLENPQEANAIIRKTLLAQEARRRSQEARELTRRKSPFDSGSLPGKLADCTTRDPSISELYIVEGDSAGGTAKTGRDRYFQAILPLRGKILNVEKSHFEQIFNNVEISALVMAVGCGIKPDFELEKLRYNKIIIMTDADVDGAHIRTLLLTFFFRFMYPLVEQGNIYIAQPPLYKVSYSNKDLYMQTDVQLEEWKQQHPNLKYNLQRYKGLGEMDAIQLWETTMDPKVRTLLKVTVEDASIADKAFSLLMGDEVPPRREFIEQNARNVKNIDI", "aa_hexdigest": "7fbdb582d3430eefa7a01fa4d02c95ce", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000012BECC", "ncbi_nrp_id": "WP_010874360.1", "uniref100_id": "UniRef100_P22447", "db_xrefs": [ "SO:0001217", "UniParc:UPI000012BECC", "RefSeq:WP_010874360.1", "UniRef:UniRef100_P22447" ] }, "ips": { "uniref100_id": "UniRef100_P22447", "uniref90_id": "UniRef90_P22447" }, "psc": { "uniref90_id": "UniRef90_P22447", "gene": "gyrB", "product": "DNA topoisomerase (ATP-hydrolyzing) subunit B", "ec_ids": [ "5.6.2.2" ], "uniref50_id": "UniRef50_P22447", "cog_id": "COG0187", "cog_category": "L", "go_ids": [ "GO:0003677", "GO:0003918", "GO:0005524", "GO:0005694", "GO:0005737", "GO:0006261", "GO:0006265", "GO:0034335", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_P22447", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P22447" ], "product": "DNA gyrase subunit B" }, "genes": [ "gyrB" ], "id": "APKECPOBEN_48", "locus": "GCA002090215_00005" }, { "type": "cds", "sequence": "contig_1", "start": 4821, "stop": 7340, "strand": "+", "frame": 3, "gene": "gyrA", "product": "DNA topoisomerase (ATP-hydrolyzing) subunit A", "db_xrefs": [ "COG:COG0188", "COG:L", "EC:5.6.2.2", "GO:0003677", "GO:0003916", "GO:0003918", "GO:0005524", "GO:0005694", "GO:0005737", "GO:0006261", "GO:0006265", "GO:0009330", "GO:0034335", "KEGG:K02469", "RefSeq:WP_015344862.1", "SO:0001217", "UniParc:UPI0002B843E9", "UniRef:UniRef100_UPI0002B843E9", "UniRef:UniRef50_P47719", "UniRef:UniRef90_P22446" ], "nt": "ATGGCAAAGCAACAAGATCAAATTGACAAAATTCGTCAGGAATTAGCACAATCAGCAATTAAAAACATCTCCCTCTCCAGTGAACTAGAACGTTCCTTCATGGAGTATGCGATGTCGGTGATAGTGGCTCGCGCGCTCCCAGATGCGAGGGATGGACTTAAACCTGTGCACCGCCGTGTGCTCTATGGTGCTTACACTGGGGGCATGCACCACGATCGCCCCTTTAAAAAGTCAGCACGGATTGTCGGTGATGTAATGAGTAAGTTCCACCCCCACGGGGACATGGCCATTTACGACACAATGTCGCGGATGGCGCAGGACTTTTCTTTGCGTTACCTCTTAATTGACGGACATGGTAACTTTGGCTCGATTGATGGCGATCGTCCCGCAGCACAACGTTATACGGAAGCACGGTTGTCGAAATTAGCGGGGGAACTGTTACGTGACATTGACAAGGACACGGTCGACTTTGTCGCTAACTATGATGGGGAAGAACAAGAACCAACGGTTTTACCAGCTGCCTTTCCTAATTTATTAGCTAATGGTTCGAGTGGGATTGCGGTAGGGATGTCCACTTCCATTCCGTCTCACAACTTATCGGAGTTAATCCAGGGCTTAATCCTTTTAATTGACAACCCTGACTGCACCATTAATGATTTATTAGGTGTCATTAAAGGACCGGACTTTCCCACCGGAGCCAACATTATTTACACCAAGGGGATTGAAAGCTACTTTGAAACTGGGAAGGGTAATGTGGTCATTCGTTCTAAAGTCAGCATTGAACAACTACCAACCCGGGCTGCTTTAGTAGTCACGGAAATTCCCTACATGGTCAACAAGACCAGTTTAATTGAAAAGATTGTCGAGTTAGTCAAGGCTGAGGAAATCACCGGGATTGCCGATATCCGTGATGAGTCATCCAGGGAAGGAATTCGCTTGGTGATTGAAGTGAAGCGCGACACCGTTCCGGAAGTCTTATTAAACCAACTGTTCAAATCAACCCGTTTACAGGTGCGGTTCCCCGTGAACATGTTGGCACTGGTTAAGGGTGCACCTAAATTGCTTAACATGAAGCAAGCGTTAACGGTTTACCTCGAGCACCAACTCGATGTTCTGATTCGCAAAACCCAGTTTAACCTCAAGAAGTACCAGGAGCGCTTTCATATCTTAAGTGGCCTTTTAATTGCGGCGTTGAACATTGATGAGGTAATTGCGATCATTAAAAAGTCCGCTAATAACCAGGTAGCAATGGAAGCACTCCACGAACGCTTCGGGTTAGATGAAATCCAAGCACGGGCGGTTCTGGACATGCGTTTACGTAGCTTAAGTGTCTTGGAAGTCAATAAACTACAAACCGAACAACAAGAGTTGAAAGCTTTAATTGAGTTCTGTCAACAGGTGTTAGCTGACAAACAACTGCAACTCAAACTAATTAAGGAGCAGTTAACCAAGATTAACGAACAGTTTGGCGATCCACGCCGTAGTGAAATCCTGTACGGTATTAGTGAAGACATTGATGATGAGGACTTAATTACCCAGGAAAACGTGGTAATCACGATGTCCACTAACGGTTATCTCAAACGGATTGGGGTAGACGCTTATAACCTGCAACACCGTGGTGGGGTGGGTGTTAAGGGTTTAACTACCTACACCGATGACAGTATTAGCCAGCTGTTAGTCTGCTCGACCCACTCGGACCTCTTATTCTTTACGGACAAGGGAAAGGTCTACCGCATCCGGGCCCACCAAATTCCCCCGGGTTTTCGTACTAACAAGGGGATTCCGGCGGTTAACCTGATTAAGATCGACAAGGACGAGAAGATCTGTGCCTTAATTTCGGTGAATGATTACCAAAATGGTTACTTCTTCTTTTGTACCAAGAACGGTACCATTAAGCGCACCAGTCTAAGTGAGTTTGCCAACATCTTGAGTATTGGTAAACGCGCCATCCTCTTTAAGGAAAACGATGTTTTGTTCTCGGTTATTCGCACCAGTGGTCAAGATGACATCTTTATCGGTTCGACCGCGGGGTTTGTCGTGCGTTTCCACGAAGACACCGTCCGTCCGCTGTCCCGCGCTGCCATGGGGGTCTTGGGGATTAATCTCAACCAGTGCGAATTTGTCAATGGTCTGTCCACTTCCAGTAACGGGAGTTTATTACTCTCTGTGGGTCAAAATGGCATTGGTAAGCTCACCAGTATTGACAAATATCGTTTAACCAAGCGGAATGCTAAGGGAGTTAAAACACTACGAGTAACTGCTAAAACTGGTCCGGTTGTGACCACTACGACCGTCTTTGGTAACGAAGATTTGTTAATGATTTCTTCAGCTGGTAAGATTGTCCGGATTAGTCTGGAACAACTTTCAGAACAGGGAAAAAACACTTCTGGTGTTAAACTAATCAAACTAAAAGAGAAGGAACGCTTAGAGACTGTTACTATCTTTAAAAAGGAAGAAGCCATTAAAACCACAACAGCAACCGAGACAGACGATGTTGGATCGAAACAAATTACGCAATAA", "aa": "MAKQQDQIDKIRQELAQSAIKNISLSSELERSFMEYAMSVIVARALPDARDGLKPVHRRVLYGAYTGGMHHDRPFKKSARIVGDVMSKFHPHGDMAIYDTMSRMAQDFSLRYLLIDGHGNFGSIDGDRPAAQRYTEARLSKLAGELLRDIDKDTVDFVANYDGEEQEPTVLPAAFPNLLANGSSGIAVGMSTSIPSHNLSELIQGLILLIDNPDCTINDLLGVIKGPDFPTGANIIYTKGIESYFETGKGNVVIRSKVSIEQLPTRAALVVTEIPYMVNKTSLIEKIVELVKAEEITGIADIRDESSREGIRLVIEVKRDTVPEVLLNQLFKSTRLQVRFPVNMLALVKGAPKLLNMKQALTVYLEHQLDVLIRKTQFNLKKYQERFHILSGLLIAALNIDEVIAIIKKSANNQVAMEALHERFGLDEIQARAVLDMRLRSLSVLEVNKLQTEQQELKALIEFCQQVLADKQLQLKLIKEQLTKINEQFGDPRRSEILYGISEDIDDEDLITQENVVITMSTNGYLKRIGVDAYNLQHRGGVGVKGLTTYTDDSISQLLVCSTHSDLLFFTDKGKVYRIRAHQIPPGFRTNKGIPAVNLIKIDKDEKICALISVNDYQNGYFFFCTKNGTIKRTSLSEFANILSIGKRAILFKENDVLFSVIRTSGQDDIFIGSTAGFVVRFHEDTVRPLSRAAMGVLGINLNQCEFVNGLSTSSNGSLLLSVGQNGIGKLTSIDKYRLTKRNAKGVKTLRVTAKTGPVVTTTTVFGNEDLLMISSAGKIVRISLEQLSEQGKNTSGVKLIKLKEKERLETVTIFKKEEAIKTTTATETDDVGSKQITQ", "aa_hexdigest": "3bcda87583d09590146f09e9bce2d185", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0002B843E9", "ncbi_nrp_id": "WP_015344862.1", "uniref100_id": "UniRef100_UPI0002B843E9", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002B843E9", "RefSeq:WP_015344862.1", "UniRef:UniRef100_UPI0002B843E9" ] }, "ips": { "uniref100_id": "UniRef100_UPI0002B843E9", "uniref90_id": "UniRef90_P22446" }, "psc": { "uniref90_id": "UniRef90_P22446", "gene": "gyrA", "product": "DNA topoisomerase (ATP-hydrolyzing) subunit A", "ec_ids": [ "5.6.2.2" ], "uniref50_id": "UniRef50_P47719", "cog_id": "COG0188", "cog_category": "L", "kegg_orthology_id": "K02469", "go_ids": [ "GO:0003677", "GO:0003916", "GO:0003918", "GO:0005524", "GO:0005694", "GO:0005737", "GO:0006261", "GO:0006265", "GO:0009330", "GO:0034335" ] }, "pscc": { "uniref50_id": "UniRef50_P47719", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47719" ], "product": "DNA gyrase subunit A" }, "genes": [ "gyrA" ], "id": "APKECPOBEN_49", "locus": "GCA002090215_00006" }, { "type": "cds", "sequence": "contig_1", "start": 7312, "stop": 7875, "strand": "+", "frame": 1, "gene": "serS", "product": "serine--tRNA ligase", "db_xrefs": [ "COG:COG0172", "COG:J", "EC:6.1.1.11", "GO:0000166", "GO:0004828", "GO:0005524", "GO:0005737", "GO:0006434", "GO:0016260", "KEGG:K01875", "SO:0001217", "UniRef:UniRef50_P47251", "UniRef:UniRef90_P75107" ], "nt": "ATGTTGGATCGAAACAAATTACGCAATAACCTTGACTTCTTTAAAAAGAAGTTAGTTGAACGGGGTGTAAGTGAAAGCCAGTTCGAGGCGTACGTGCAAGCTGATAAAGCAATGCGCAAACTGTTGCACCAAATTGAACTGGCCAACCAAAAGCAAACCCTGTTAGCGCAACAGGTTGCCAAGAAGAAGGGTGATCCGAAGTTATTAAAGGAATCCAAGGAGTTAAAGCAAAAACTAGAACAGCTCAACATCGCTTTTAAGGAAGCGGAAACCTTAAGCCAAGAGTTACTGTTAAACCTCCCTAACATTGCTGATGAATCAGTACCAGTTGGACGGGATGAAACTGCTAACTTAGAACTGCTCAAAGAAGGGCGTAAACCGGTGTTTGACTTTACTCCCTTACCCCACTGGGAGTTGTGTGAACGCTTACAGTTAGTTGCCTTTGATAAGGCCACTAAGCTAACGGGTGCACGGTTTGTCGCTTACACTGATAAAGCAGCGAAACTGCTCCGCGCCATTGCTAGCTTAATGATTGACCTCAACAAAAACAAATACCAGGAGTGA", "aa": "MLDRNKLRNNLDFFKKKLVERGVSESQFEAYVQADKAMRKLLHQIELANQKQTLLAQQVAKKKGDPKLLKESKELKQKLEQLNIAFKEAETLSQELLLNLPNIADESVPVGRDETANLELLKEGRKPVFDFTPLPHWELCERLQLVAFDKATKLTGARFVAYTDKAAKLLRAIASLMIDLNKNKYQE", "aa_hexdigest": "6faf8b3d9dfca325baf2ecbd7d7c7ba3", "start_type": "ATG", "rbs_motif": null, "genes": [ "serS" ], "truncated": "3-prime", "pseudogene": { "start": 7312, "stop": 8178, "observations": { "mutation": [ 7873 ], "stop": [ 7873 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75107", "query_cov": 1.0, "subject_cov": 0.4452380952380952, "identity": 1.0, "score": 350.0, "evalue": 3.45e-118, "gene_start": 1, "gene_end": 187, "reference_start": 1, "reference_end": 187, "reference_sequence": "MLDRNKLRNNLDFFKKKLVERGVSESQFEAYVQADKAMRKLLHQIELANQKQTLLAQQVAKKKGDPKLLKESKELKQKLEQLNIAFKEAETLSQELLLNLPNIADESVPVGRDETANLELLKEGRKPVFDFTPLPHWELCERLQLVAFDKATKLTGARFVAYTDKAAKLLRAIASLMIDLNKNKYQEWNVPVIVNETSLTGTGQLPKFKDDVFKLENTRYYLSPTLEVQLANLHANEIFTEGELPKYYTATGVNFRQEAGSAGKQTKGTIRLHQFQKVELVKFCKPSEAIHELEEMTRDAEQILLELKIPFRRLLLCSGDMGFSAQKTYDLEVWMAGCNEYREVSSCSSCGDFQARRAMIRYKDLTTGKNTYVATLNGTALAIDRIFAAILEHYQTKAGEVMIPQALLKYLDFDKITKPK" }, "paralog": false, "identity": 0.9965277777777778, "score": 562.0, "evalue": 4.69e-204, "description": "Internal stop codon at 7873. Nonsense mutation around 7873.", "psc": { "uniref90_id": "UniRef90_P75107", "gene": "serS", "product": "serine--tRNA ligase", "ec_ids": [ "6.1.1.11" ], "uniref50_id": "UniRef50_P47251", "cog_id": "COG0172", "cog_category": "J", "kegg_orthology_id": "K01875", "go_ids": [ "GO:0000166", "GO:0004828", "GO:0005524", "GO:0005737", "GO:0006434", "GO:0016260" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75107", "EC:6.1.1.11", "UniRef:UniRef50_P47251", "COG:COG0172", "COG:J", "KEGG:K01875", "GO:0000166", "GO:0004828", "GO:0005524", "GO:0005737", "GO:0006434", "GO:0016260" ] }, "pscc": { "uniref50_id": "UniRef50_P47251", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47251" ], "product": "Serine--tRNA ligase" } }, "id": "APKECPOBEN_50", "locus": "GCA002090215_00007" }, { "type": "cds", "sequence": "contig_1", "start": 7879, "stop": 8574, "strand": "+", "frame": 1, "gene": null, "product": "Serine--tRNA ligase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A540V7F7" ], "nt": "GTGCCGGTCATTGTGAACGAGACTAGTCTTACTGGTACGGGTCAGCTACCCAAATTTAAGGATGATGTCTTTAAGTTAGAAAACACGCGTTACTACCTCTCCCCGACCCTAGAGGTGCAACTAGCCAACCTCCATGCCAACGAAATCTTCACGGAGGGTGAACTCCCCAAATACTATACCGCTACTGGGGTGAACTTTCGCCAGGAAGCGGGCAGTGCTGGTAAACAAACTAAGGGTACGATTCGCCTACACCAGTTCCAAAAGGTAGAACTAGTGAAGTTTTGTAAACCAAGTGAAGCGATTCACGAACTGGAAGAAATGACCCGGGACGCTGAGCAAATTTTGTTAGAACTCAAAATTCCCTTTCGCCGTTTGTTACTCTGTAGCGGTGACATGGGTTTTAGTGCCCAGAAAACCTATGACTTGGAAGTGTGGATGGCGGGTTGCAATGAGTACCGCGAAGTTTCGTCCTGTTCTTCTTGTGGTGACTTTCAAGCACGCAGAGCAATGATCCGCTATAAGGACTTAACCACAGGCAAGAATACCTATGTGGCTACCTTAAACGGCACCGCTTTAGCGATTGACCGCATCTTTGCAGCCATCTTGGAACACTACCAAACGAAGGCAGGGGAAGTAATGATTCCCCAAGCCTTGTTGAAATACTTAGACTTTGACAAAATTACCAAACCAAAATAA", "aa": "MPVIVNETSLTGTGQLPKFKDDVFKLENTRYYLSPTLEVQLANLHANEIFTEGELPKYYTATGVNFRQEAGSAGKQTKGTIRLHQFQKVELVKFCKPSEAIHELEEMTRDAEQILLELKIPFRRLLLCSGDMGFSAQKTYDLEVWMAGCNEYREVSSCSSCGDFQARRAMIRYKDLTTGKNTYVATLNGTALAIDRIFAAILEHYQTKAGEVMIPQALLKYLDFDKITKPK", "aa_hexdigest": "8ec45be77fe2508c618d0add3afe44e1", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_C4FJT5", "query_cov": 0.9567099567099567, "subject_cov": 0.8804780876494024, "identity": 0.588, "score": 255.0, "evalue": 1.68e-82, "valid": false, "gene": "serS", "product": "serine--tRNA ligase", "ec_ids": [ "6.1.1.11" ], "uniref50_id": "UniRef50_A0A540V7F7", "kegg_orthology_id": "K01875", "go_ids": [ "GO:0000166", "GO:0004828", "GO:0005524", "GO:0006434" ] }, "pscc": { "uniref50_id": "UniRef50_A0A540V7F7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A540V7F7" ], "product": "Serine--tRNA ligase" }, "genes": [], "id": "APKECPOBEN_51", "locus": "GCA002090215_00008" }, { "type": "cds", "sequence": "contig_1", "start": 8579, "stop": 8875, "strand": "+", "frame": 2, "gene": "tmk", "product": "dTMP kinase", "db_xrefs": [ "COG:COG0125", "COG:F", "EC:2.7.4.9", "GO:0004798", "GO:0005524", "GO:0005829", "GO:0006227", "GO:0006233", "GO:0006235", "KEGG:K00943", "SO:0001217", "UniRef:UniRef50_P47252", "UniRef:UniRef90_P75106" ], "nt": "ATGAAACAAGGCGTGTTTGTGGCCATAGAGGGCGTGGATGGTGCGGGGAAAACCGTCCTCCTAGAAGCTTTTAAACAGCGCTTTCCCCAAAGCTTTTTGGGCTTTAAAACGCTTTTTAGCAGGGAACCCGGGGGCACACCGTTAGCGGAAAAAATCCGGGCACTGCTCCTGCATGAAGCAATGGAACCACTTACAGAAGCTTATCTGTTTGCGGCATCGCGCACCGAACACGTGCGCCAGTTAATCCAACCAGCGTTACAACAAAAACAGTTAGTGATAGTAGACCGCTTTGTGTGA", "aa": "MKQGVFVAIEGVDGAGKTVLLEAFKQRFPQSFLGFKTLFSREPGGTPLAEKIRALLLHEAMEPLTEAYLFAASRTEHVRQLIQPALQQKQLVIVDRFV", "aa_hexdigest": "2021ebc7f345e281ef75aac8228ee1cf", "start_type": "ATG", "rbs_motif": null, "genes": [ "tmk" ], "truncated": "3-prime", "pseudogene": { "start": 8579, "stop": 9178, "observations": { "mutation": [ 8873 ], "stop": [ 8873 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75106", "query_cov": 1.0, "subject_cov": 0.4666666666666667, "identity": 1.0, "score": 191.0, "evalue": 3.93e-60, "gene_start": 1, "gene_end": 98, "reference_start": 1, "reference_end": 98, "reference_sequence": "MKQGVFVAIEGVDGAGKTVLLEAFKQRFPQSFLGFKTLFSREPGGTPLAEKIRALLLHEAMEPLTEAYLFAASRTEHVRQLIQPALQQKQLVIVDRFVWSSYAYQGLIKKVGLDVVKKLNADAVGDSMPDFTFIVDCDFETALNRMAKRGQDNLLDNTVKKQADFNTMRQYYHSLVDNKRVFLLDGQNQTGCLEQFIEQLSQCLTQPTLS" }, "paralog": false, "identity": 0.9949748743718593, "score": 390.0, "evalue": 4.07e-141, "description": "Internal stop codon at 8873. Nonsense mutation around 8873.", "psc": { "uniref90_id": "UniRef90_P75106", "gene": "tmk", "product": "dTMP kinase", "ec_ids": [ "2.7.4.9" ], "uniref50_id": "UniRef50_P47252", "cog_id": "COG0125", "cog_category": "F", "kegg_orthology_id": "K00943", "go_ids": [ "GO:0004798", "GO:0005524", "GO:0005829", "GO:0006227", "GO:0006233", "GO:0006235" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75106", "EC:2.7.4.9", "UniRef:UniRef50_P47252", "COG:COG0125", "COG:F", "KEGG:K00943", "GO:0004798", "GO:0005524", "GO:0005829", "GO:0006227", "GO:0006233", "GO:0006235" ] }, "pscc": { "uniref50_id": "UniRef50_P47252", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47252" ], "product": "Thymidylate kinase" } }, "id": "APKECPOBEN_52", "locus": "GCA002090215_00009" }, { "type": "cds", "sequence": "contig_1", "start": 8921, "stop": 9211, "strand": "+", "frame": 2, "gene": "tmk", "product": "dTMP kinase", "db_xrefs": [ "COG:COG0125", "COG:F", "EC:2.7.4.9", "GO:0004798", "GO:0005524", "GO:0005829", "GO:0006227", "GO:0006233", "GO:0006235", "KEGG:K00943", "SO:0001217", "UniRef:UniRef50_P47252", "UniRef:UniRef90_P75106" ], "nt": "GTGGTCAAAAAGCTCAATGCTGATGCGGTGGGGGATAGTATGCCCGACTTTACCTTTATTGTTGATTGTGACTTTGAGACTGCTTTAAACCGGATGGCCAAACGCGGTCAGGACAATTTATTGGACAATACTGTTAAAAAGCAAGCGGACTTTAATACTATGCGCCAGTATTACCACAGTTTGGTTGATAACAAACGCGTCTTTCTCTTAGACGGGCAAAACCAAACGGGTTGTCTGGAACAATTTATTGAACAATTAAGCCAATGTTTAACCCAACCCACGCTCTCTTAA", "aa": "MVKKLNADAVGDSMPDFTFIVDCDFETALNRMAKRGQDNLLDNTVKKQADFNTMRQYYHSLVDNKRVFLLDGQNQTGCLEQFIEQLSQCLTQPTLS", "aa_hexdigest": "267853f6b6a38c42afecbf2e79d4afa8", "start_type": "GTG", "rbs_motif": "AAAA", "genes": [ "tmk" ], "truncated": "5-prime", "pseudogene": { "start": 8621, "stop": 9211, "observations": { "mutation": [ 8873 ], "stop": [ 8873 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75106", "query_cov": 1.0, "subject_cov": 0.45714285714285713, "identity": 0.99, "score": 193.0, "evalue": 4.43e-61, "gene_start": 1, "gene_end": 96, "reference_start": 115, "reference_end": 210, "reference_sequence": "MKQGVFVAIEGVDGAGKTVLLEAFKQRFPQSFLGFKTLFSREPGGTPLAEKIRALLLHEAMEPLTEAYLFAASRTEHVRQLIQPALQQKQLVIVDRFVWSSYAYQGLIKKVGLDVVKKLNADAVGDSMPDFTFIVDCDFETALNRMAKRGQDNLLDNTVKKQADFNTMRQYYHSLVDNKRVFLLDGQNQTGCLEQFIEQLSQCLTQPTLS" }, "paralog": false, "identity": 0.9948979591836735, "score": 385.0, "evalue": 5.09e-139, "description": "Internal stop codon at 8873. Nonsense mutation around 8873.", "psc": { "uniref90_id": "UniRef90_P75106", "gene": "tmk", "product": "dTMP kinase", "ec_ids": [ "2.7.4.9" ], "uniref50_id": "UniRef50_P47252", "cog_id": "COG0125", "cog_category": "F", "kegg_orthology_id": "K00943", "go_ids": [ "GO:0004798", "GO:0005524", "GO:0005829", "GO:0006227", "GO:0006233", "GO:0006235" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75106", "EC:2.7.4.9", "UniRef:UniRef50_P47252", "COG:COG0125", "COG:F", "KEGG:K00943", "GO:0004798", "GO:0005524", "GO:0005829", "GO:0006227", "GO:0006233", "GO:0006235" ] }, "pscc": { "uniref50_id": "UniRef50_P47252", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47252" ], "product": "Thymidylate kinase" } }, "id": "APKECPOBEN_53", "locus": "GCA002090215_00010" }, { "type": "cds", "sequence": "contig_1", "start": 9184, "stop": 9942, "strand": "+", "frame": 1, "gene": "holB", "product": "DNA polymerase III subunit delta'", "db_xrefs": [ "COG:COG0470", "COG:L", "EC:2.7.7.7", "SO:0001217", "UniRef:UniRef50_P47253", "UniRef:UniRef90_P75105" ], "nt": "ATGTTTAACCCAACCCACGCTCTCTTAATTATTCAAAGACGGGGTAGCTATCTCCAACCAGTTTTAACGGAGTACCTAACCCGGGTCGTTTGTGAACAGCAAACGGGGTGTCAAACATGTCCGAGTTGCTTGGAAATTCTCCATGGTACTTACAACAACTTTTACAGTTTTGACCAAGCCAACCCCTTTAAAAGGGAACATGCCTTGCACCTTAGTGAAGTGTTGAACCGCCAAAGTGAATCCAACCAAAAACAGCTGTACCTGATTAAAAACTTAGAAACGCTCACCGCTACGGCCATGAACAGCTTGCTCCGTTTAATTGAGGAACACCCGGTGAATACCTATGGTGTTTTTACCACGAAAAACGAAAACATGATCTTACCCACCATTTTGAGTCGCGTCCAAAAAGTAGTACTCAAAAAAGCGACCCAGTTACCGTTCCAAGTGGACAGTAAGGACCAGGCGATTCTCAAAAGTTTCTTTAGTGTAGATGAACAGTTACAAGCATTGGACAACGGTAGTTTTACCCGCTTAAAAACGATTATTACGACACTTACCAATAAGAAAAACACAGCGAGCACGGTTCACGAGGCTTGGGTGTTGTTTAAACAATTAAACCAAACGGAAACGGCTCAAGTACTGAACTTTATGGTGGATTACACCAAGGACTTAACCAAAAAGGACCGCTTGTTAAACATGGTGCAAAATTTGGTGTTTAACCCACCGAAAGCAGCGTTGTTTGCTAACCTCATTAACTGA", "aa": "MFNPTHALLIIQRRGSYLQPVLTEYLTRVVCEQQTGCQTCPSCLEILHGTYNNFYSFDQANPFKREHALHLSEVLNRQSESNQKQLYLIKNLETLTATAMNSLLRLIEEHPVNTYGVFTTKNENMILPTILSRVQKVVLKKATQLPFQVDSKDQAILKSFFSVDEQLQALDNGSFTRLKTIITTLTNKKNTASTVHEAWVLFKQLNQTETAQVLNFMVDYTKDLTKKDRLLNMVQNLVFNPPKAALFANLIN", "aa_hexdigest": "b3da2a21c3b77df301930721acf99695", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75105", "query_cov": 1.0, "subject_cov": 0.9960474308300395, "identity": 1.0, "score": 494.0, "evalue": 1.25e-176, "valid": true, "gene": "HolB", "product": "DNA polymerase III subunit delta'", "ec_ids": [ "2.7.7.7" ], "uniref50_id": "UniRef50_P47253", "cog_id": "COG0470", "cog_category": "L" }, "pscc": { "uniref50_id": "UniRef50_P47253", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47253" ], "product": "Uncharacterized protein MG007" }, "genes": [ "holB" ], "id": "APKECPOBEN_54", "locus": "GCA002090215_00011" }, { "type": "cds", "sequence": "contig_1", "start": 9947, "stop": 11275, "strand": "+", "frame": 2, "gene": "mnmE", "product": "tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE", "db_xrefs": [ "COG:COG0486", "COG:J", "EC:3.6.-.-", "GO:0002098", "GO:0003924", "GO:0005525", "GO:0005829", "GO:0006400", "GO:0030488", "GO:0046872", "RefSeq:WP_010874365.1", "SO:0001217", "UniParc:UPI0000137451", "UniRef:UniRef100_P75104", "UniRef:UniRef50_P47254", "UniRef:UniRef90_P75104" ], "nt": "ATGGACACCAAACAAACCATGTTTGCCCTCGCTACTGCGCCCTTCAACAGTGCCATTCACATTATTAGGTTATCTGGTCCCGATGTTTATCGCATTATTAACCAAATAACCAATAAGGAAGTCAAACCGTTGGGTATGCGGATTCAACGCGTTTGGTTAATTGATCACAACCAGAAAAAAGTGGATGATGTGTTGCTGTTTAAATTTGTCGCACCTAACTCTTACACTGGGGAAGATCTGATTGAAATTTCGTGCCATGGCAGCATGGTAATAGTCAACGAAATTATTGGTTTATTGTTAAAGCACGGGGCGGTACAAGCCCAACCCGGGGAGTTTACGCAGCGCGGCTATCTCAACGGTAAGATGAGTTTAAACCAAGCGGCTTCTGTTAATAACTTAGTACTTTCACCGAATACAACCCTAAAGGATGTCGCTTTAAATGCGTTAGCTGGTCAAGTTGATGCCAGGTTAGAACCCTTAGTGGAAAAGTTGGGTCAACTAGTAATGCAAATGGAAGTGAACTTGGATTACCCCGAGTACACTGATGAACAGCGCGAACTGGTGACAATGAACCAAGCAGTCGTGCAAATAACCCAAATCTTAAACCAAATCGTGGTTGGTCAAGACCAGTTACAACGGCTCAAAGATCCGTTTAAGATTGCCATTATTGGTAATACTAATGTTGGTAAGTCCTCCTTATTGAATGCTCTGTTAGATCAAGATAAAGCGATAGTATCCGCCATTAAAGGCAGTACCCGCGACATTGTTGAGGGTGACTTTGCTCTAAACGGTCACTTCGTCAAAATCCTCGACACAGCGGGAATTCGCCAACACCAAAGTGCGCTCGAAAAAGCGGGCATTCAAAAAACCTTTGGCGCCATTAAAACCGCTAACTTAGTAATTTACCTTTTGGATGCCCGGCAGCCAGAACCTGATCCCAAGATTATTGCGCGATTAAAGAAGCTGAAGAAGGACTTCTTTTTGGTCCACAATAAAGCAGACTTAGTGCAGCAAAGCTTTCAGGTAAGTATTTCTGCCAAACAAAAACAGATCCAACCACTTGTCGATTTATTAACCCAATACCTACACCAGTTTTACAGTGTGGAGCAGAACCAGCTCTACTTAATTAGTGATTGGCAAACGATCCTGCTCCAAAAAGCAATAGCCGAACTAGAACATTTTTTAATTAAACAACAAAACTGCCTTTTCTTTGATATCCTAGTTGTTCATTTACGTGCTGCTCACGAATACATTCTCCAAGTCTTGGGTAAAAACACCAACTATGACCTGATTAACGAAATCTTTAAGCACTTTTGCTTAGGTAAGTAA", "aa": "MDTKQTMFALATAPFNSAIHIIRLSGPDVYRIINQITNKEVKPLGMRIQRVWLIDHNQKKVDDVLLFKFVAPNSYTGEDLIEISCHGSMVIVNEIIGLLLKHGAVQAQPGEFTQRGYLNGKMSLNQAASVNNLVLSPNTTLKDVALNALAGQVDARLEPLVEKLGQLVMQMEVNLDYPEYTDEQRELVTMNQAVVQITQILNQIVVGQDQLQRLKDPFKIAIIGNTNVGKSSLLNALLDQDKAIVSAIKGSTRDIVEGDFALNGHFVKILDTAGIRQHQSALEKAGIQKTFGAIKTANLVIYLLDARQPEPDPKIIARLKKLKKDFFLVHNKADLVQQSFQVSISAKQKQIQPLVDLLTQYLHQFYSVEQNQLYLISDWQTILLQKAIAELEHFLIKQQNCLFFDILVVHLRAAHEYILQVLGKNTNYDLINEIFKHFCLGK", "aa_hexdigest": "52e8df3d7dc2750a77960cb17c92e05a", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000137451", "ncbi_nrp_id": "WP_010874365.1", "uniref100_id": "UniRef100_P75104", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000137451", "RefSeq:WP_010874365.1", "UniRef:UniRef100_P75104" ] }, "ips": { "uniref100_id": "UniRef100_P75104", "uniref90_id": "UniRef90_P75104" }, "psc": { "uniref90_id": "UniRef90_P75104", "gene": "mnmE", "product": "tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE", "ec_ids": [ "3.6.-.-" ], "uniref50_id": "UniRef50_P47254", "cog_id": "COG0486", "cog_category": "J", "go_ids": [ "GO:0002098", "GO:0003924", "GO:0005525", "GO:0005829", "GO:0006400", "GO:0030488", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_P47254", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47254" ], "product": "tRNA modification GTPase MnmE" }, "genes": [ "mnmE" ], "id": "APKECPOBEN_55", "locus": "GCA002090215_00012" }, { "type": "cds", "sequence": "contig_1", "start": 11275, "stop": 11952, "strand": "+", "frame": 1, "gene": "tatD", "product": "putative metal-dependent hydrolase MPN_009", "db_xrefs": [ "COG:COG0084", "COG:N", "EC:3.1.-.-", "GO:0004536", "GO:0005829", "GO:0046872", "KEGG:K03424", "SO:0001217", "UniRef:UniRef50_P47255", "UniRef:UniRef90_P78005" ], "nt": "ATGGAATACTTTGATGCGCACTGTCACTTAAACTGTGAACCCCTACTGGAACAGCACGAAAAGAGTTTGGCCAACTTTAGGTTAATTGGGTTAAAGGCCAATGTTGTGGGCACTAATTTAACTAACTCCCAAATTGCGGTGAATTTAGCTAAACAGCACCCTGATTTACTCAAGGCAGGGGTGGGTATTCACCCTAATGATGTCCAGCTTTTTGATTTAAAAGCAGCGCAAGCAACACTAAAAAAATTAGTAAGTACGCATCGCTCATTCATTAGTTGTATTGGTGAATATGGCTTTGATTACCACTACACTAAGGACTACATTACCCAACAAGAACAGTTCTTTTTGATGCAGTTCCAACTAGCAGAACAGTACCAGTTAGTGCACATGTTGCATGTCCGTGATGTGCACGAAAGGATATACGAAGTTTTGAAAAGGTTAAAACCTAAACAACCAGTGGTGTTTCACTGTTTTAGTGAAGACACCAATACTGCTTTAAAGCTGCTAACTTTAAGAGAAGTAGGACTAAAGGTTTATTTTTCGATTCCGGGGATTGTGACCTTTAAGAATGCGAAAAACTTGCAAGCCGCTTTATCAGTTATTCCAACAGAGCTACTTTTAAGTGAAACCGATTCGCCTTATTTAGCACCAGTACCTTTTCGGGGCAAAACTAATTGA", "aa": "MEYFDAHCHLNCEPLLEQHEKSLANFRLIGLKANVVGTNLTNSQIAVNLAKQHPDLLKAGVGIHPNDVQLFDLKAAQATLKKLVSTHRSFISCIGEYGFDYHYTKDYITQQEQFFLMQFQLAEQYQLVHMLHVRDVHERIYEVLKRLKPKQPVVFHCFSEDTNTALKLLTLREVGLKVYFSIPGIVTFKNAKNLQAALSVIPTELLLSETDSPYLAPVPFRGKTN", "aa_hexdigest": "a0fdfb04770795cdc13d29507142cb79", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P78005", "query_cov": 1.0044444444444445, "subject_cov": 0.8625954198473282, "identity": 0.996, "score": 444.0, "evalue": 2.67e-157, "valid": true, "gene": "TatD", "product": "Uncharacterized metal-dependent hydrolase MPN_009", "ec_ids": [ "3.1.-.-" ], "uniref50_id": "UniRef50_P47255", "cog_id": "COG0084", "cog_category": "N", "kegg_orthology_id": "K03424", "go_ids": [ "GO:0005829", "GO:0004536", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_P47255", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47255" ], "product": "Uncharacterized metal-dependent hydrolase MG009" }, "genes": [ "tatD" ], "id": "APKECPOBEN_56", "locus": "GCA002090215_00013" }, { "type": "cds", "sequence": "contig_1", "start": 12257, "stop": 12367, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTTACTCGCCTTCGCTTAACGACATTAAGAGCATTTTGAACAAATATACCTCTAAGGACTATGAGTTAAAATGCGAAAATAGGTATGATGGCAAGCTTGAATTATGA", "aa": "MAYSPSLNDIKSILNKYTSKDYELKCENRYDGKLEL", "aa_hexdigest": "ef6ad5242060c2922cad14c787ae9fd9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4243.7669000000005, "isoelectric_point": 6.057411003112793 }, "id": "APKECPOBEN_57", "locus": "GCA002090215_00014" }, { "type": "cds", "sequence": "contig_1", "start": 12392, "stop": 12652, "strand": "+", "frame": 2, "gene": null, "product": "DUF16 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD6444", "UniRef:UniRef100_A0A0H3DL80", "UniRef:UniRef50_A0A0H3DL80", "UniRef:UniRef90_A0A0H3DL80" ], "nt": "GTGAAAACACCTGGCACTAGGTACGTCACCCATAAGCAACTTGATGAAAAGCTCAAAAATTTTGTCACTAAAACAGAATTTAAAGAGTTCCAAACTGTAGTTATGGAGAGTTTTGCTGTTCAAAACCAGAATATTGATGCTCAGGGTGAGCAGATCAAAGAACTTCAAGTTGAACAAAAAGCTCAAGGAAAGACCCTGCAATTGATACTTGAAGCTCTCCAGGGTATAAACAAACGTCTAGACAACTTAGAGTCCAAATAA", "aa": "MKTPGTRYVTHKQLDEKLKNFVTKTEFKEFQTVVMESFAVQNQNIDAQGEQIKELQVEQKAQGKTLQLILEALQGINKRLDNLESK", "aa_hexdigest": "75de747e0fb9f4f2ad11881c86013141", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD6444", "uniref100_id": "UniRef100_A0A0H3DL80", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD6444", "UniRef:UniRef100_A0A0H3DL80" ] }, "ips": { "uniref100_id": "UniRef100_A0A0H3DL80", "uniref90_id": "UniRef90_A0A0H3DL80" }, "psc": { "uniref90_id": "UniRef90_A0A0H3DL80", "product": "DUF16 domain-containing protein", "uniref50_id": "UniRef50_A0A0H3DL80" }, "pscc": { "uniref50_id": "UniRef50_A0A0H3DL80", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0H3DL80" ], "product": "DUF16 domain-containing protein" }, "genes": [], "id": "APKECPOBEN_58", "locus": "GCA002090215_00015" }, { "type": "cds", "sequence": "contig_1", "start": 12960, "stop": 13124, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAATTATTCTTACAGAGGATTCAATATCCACAAAGGTGACAAACATTATTTCAACAGTTTCCTTACTCTTAAAGCGAATTCATATTTACCATTTACGTCAAAAAATTTTGATGTCTATTCCAAAAGAATTAGGTTATCTGTTTCGTTTCATTGAATACTAA", "aa": "MKIILTEDSISTKVTNIISTVSLLLKRIHIYHLRQKILMSIPKELGYLFRFIEY", "aa_hexdigest": "258acaabdd536dd76234507b2e94fe36", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6421.700299999997, "isoelectric_point": 9.774519157409667 }, "id": "APKECPOBEN_59", "locus": "GCA002090215_00016" }, { "type": "cds", "sequence": "contig_1", "start": 13300, "stop": 13533, "strand": "-", "frame": 1, "gene": null, "product": "lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0019135978", "UniRef:UniRef90_UPI0019135978" ], "nt": "ATGAAATTTAAATTCTTATTAACCCCACTATTATCTTCAGTTTTATTTCTTAGTGCTTGTAGCGCCACTTTCGAAGCTGATCTCAAAAATCTTATTAAGGAAACAGATGGTAAAGACCTTGATGTTTCTAAATTAATTATTACTTCGGAAGGGAAACAAATCTTAATAGGTTATTTAAAAAAATCTTACGAAGTTAACTCTGAAAAAACTACTGAACTATTACTAAATGCCTGA", "aa": "MKFKFLLTPLLSSVLFLSACSATFEADLKNLIKETDGKDLDVSKLIITSEGKQILIGYLKKSYEVNSEKTTELLLNA", "aa_hexdigest": "e623a3d940a2248e5c58d780707e6d4c", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 13240, "stop": 13518, "observations": { "mutation": [ 13254, 13302 ], "stop": [ 13254, 13302 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0019135978", "query_cov": 0.935064935064935, "subject_cov": 0.7741935483870968, "identity": 1.0, "score": 135.0, "evalue": 5.17e-40, "gene_start": 6, "gene_end": 77, "reference_start": 2, "reference_end": 73, "reference_sequence": "MLLTPLLSSVLFLSACSATFEADLKNLIKETDGKDLDVSKLIITSEGKQILIGYLKKSYEVNSEKTTELLLNAWKQSQEKNQIGIDLFNWTKS" }, "paralog": false, "identity": 0.9565217391304348, "score": 166.0, "evalue": 1.24e-54, "description": "Internal stop codon at 13254, 13302. Nonsense mutation around 13254, 13302.", "psc": { "uniref90_id": "UniRef90_UPI0019135978", "product": "lipoprotein", "uniref50_id": "UniRef50_UPI0019135978", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0019135978", "UniRef:UniRef50_UPI0019135978" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0019135978", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0019135978" ], "product": "lipoprotein" } }, "id": "APKECPOBEN_60", "locus": "GCA002090215_00017" }, { "type": "cds", "sequence": "contig_1", "start": 13705, "stop": 13917, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAATATCAAGATCTTAATAATTTTTCGATAAATGCTAAGTTGAACTATAACTTTAATTGGCATGGAAATTATTCTTCCAATGGGTTTGCTGCCAAAAAAGGTGACAAACATGTTTTTGATCTTCCTCTTGCAATTAAGCCATCTTCAAAGAACCAATCTAAAACGATAAGTTTCATTACTAAGACAGGGGAAGGCGTTGACACAGAATGA", "aa": "MEYQDLNNFSINAKLNYNFNWHGNYSSNGFAAKKGDKHVFDLPLAIKPSSKNQSKTISFITKTGEGVDTE", "aa_hexdigest": "a387d55e91d6b487754fcba3a42cbbf1", "start_type": "ATG", "rbs_motif": "AAAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7872.618200000002, "isoelectric_point": 8.00570011138916 }, "id": "APKECPOBEN_61", "locus": "GCA002090215_00018" }, { "type": "cds", "sequence": "contig_1", "start": 14035, "stop": 14265, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001FCEF156", "PFAM:PF00938.23" ], "nt": "ATGAAATTTAAATACCTTTCAATTCCGTTTTTATCTTCAACTTTATTGTTTAGTGCTTATGCTTCTATCCAGTCTGATCTAAGAAATCTTATTCAAGAAACCACTAAACAAGACGTTGATGTGTACAAAGTAATCAAAACTTCTGAAGGCAGAAAAAACTTAATAACTTCTCTAAAGAAATCTTACGAAGTTAATCCTGATAAAACCACAAAACTATTGTTAGATGCCTGA", "aa": "MKFKYLSIPFLSSTLLFSAYASIQSDLRNLIQETTKQDVDVYKVIKTSEGRKNLITSLKKSYEVNPDKTTKLLLDA", "aa_hexdigest": "7003eb05d30df8ae726557f53f8a5aab", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001FCEF156", "query_cov": 0.9473684210526315, "subject_cov": 0.9863013698630136, "identity": 0.708, "score": 94.7, "evalue": 4.09e-24, "valid": false, "uniref50_id": "UniRef50_UPI001FCEF156" }, "pscc": { "uniref50_id": "UniRef50_UPI001FCEF156", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001FCEF156" ] }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00938.23", "name": "Lipoprotein_3", "length": 32, "aa_cov": 0.42105263157894735, "hmm_cov": 1.0, "evalue": 1.344277245810318e-06, "score": 23.492643356323242, "start": 45, "stop": 76 } ], "seq_stats": { "molecular_weight": 8689.978299999995, "isoelectric_point": 9.435415458679199 }, "id": "APKECPOBEN_62", "locus": "GCA002090215_00019" }, { "type": "cds", "sequence": "contig_1", "start": 14777, "stop": 14992, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W8J2", "UniRef:UniRef50_UPI001F2909C5", "UniRef:UniRef90_A0AB38W8J2", "UniRef:UniRef90_UPI001F2909C5" ], "nt": "ATGTTGTTTTGTTTGAAGTCTCTTTACTTTAAATTGCAAAAATTTTTAGCAATTTTATTGGCAGTTTTTGAAATTCTGGTCAATTTATTTAGTGAAGGAAAAATTATGTCTTTACTCAATATATTTATTAGATCAACGATGATGTTGATCCTTGCTGGCAACAAAGTTGCACGTTTTAGTTTTCTAAACCTTGAGCAGAAAGGAGGAATTTATTAA", "aa": "MLFCLKSLYFKLQKFLAILLAVFEILVNLFSEGKIMSLLNIFIRSTMMLILAGNKVARFSFLNLEQKGGIY", "aa_hexdigest": "5f40cb62e9d57c2091e2847734f24529", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001F2909C5", "query_cov": 0.9295774647887324, "subject_cov": 0.9850746268656716, "identity": 1.0, "score": 119.0, "evalue": 4.88e-34, "valid": true, "uniref50_id": "UniRef50_UPI001F2909C5" }, "pscc": { "uniref50_id": "UniRef50_UPI001F2909C5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F2909C5" ] }, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 14699, "stop": 14992, "observations": { "mutation": [ 14759 ], "stop": [ 14759 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB38W8J2", "query_cov": 1.0, "subject_cov": 0.7319587628865979, "identity": 1.0, "score": 127.0, "evalue": 5.08e-37, "gene_start": 1, "gene_end": 71, "reference_start": 27, "reference_end": 97, "reference_sequence": "HLRDVNTSLMFLNTLKNLFGWLGSLLMLFCLKSLYFKLQKFLAILLAVFEILVNLFSEGKIMSLLNIFIRSTMMLILAGNKVARFSFLNLEQKGGIY" }, "paralog": false, "identity": 0.9896907216494846, "score": 184.0, "evalue": 1.3e-61, "description": "Internal stop codon at 14759. Nonsense mutation around 14759.", "psc": { "uniref90_id": "UniRef90_A0AB38W8J2", "uniref50_id": "UniRef50_A0AB38W8J2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB38W8J2", "UniRef:UniRef50_A0AB38W8J2" ] }, "pscc": { "uniref50_id": "UniRef50_A0AB38W8J2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W8J2" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 8166.962399999995, "isoelectric_point": 9.806624412536618 }, "id": "APKECPOBEN_63", "locus": "GCA002090215_00020" }, { "type": "cds", "sequence": "contig_1", "start": 14992, "stop": 15765, "strand": "+", "frame": 1, "gene": null, "product": "UPF0134 protein MPN_013", "db_xrefs": [ "RefSeq:WP_010874370.1", "SO:0001217", "UniParc:UPI0000139F0E", "UniRef:UniRef100_P75100", "UniRef:UniRef50_P75100", "UniRef:UniRef90_P75100" ], "nt": "ATGCCTAAAGTTATTTCCCAAAAGGAATTTAATATCCTTTCCCGGACAAAGGAAAGAGTTAATTTTCAAATTGTCTATACCAAGCAAAGAAATGGTTTGCACAAAGCTGCTAAGGAGTACTTTTTTAAAGACAAAGACTTTACAATAATAGAAGATACACCGGACACACCTGACCAACCAGATGGACCATGTATTACTAAAGGCCCAAAGGAACCAGGTGGTCCTGAAGAGCCAAATGGTCCGGATAAACCAGAAGATCCTAAAGATCCTGATACGCCAGATGTTTCTGAAGGCCCTAAAGAACAAGGTGGTCAGAAAGATCCAGGTGGTCCGGATGATCCCAGTGAAGGAAAGCAGAAAGTGTCCAAACCAGATAAATACGTAACCCACCGTGAGCTTGATGAAAAGCTTAAAGACTTTGCCACCAAAGCTGATTTCAAACGCGTCGAAGACAAAGTTGATGTTTTATTTGAACTGCAAAAAACGCAAGGCGAACAAATAAAGGTTCAAGGTGAGCAGATCAAAGCTCAAGGAAAACAAATTGAACAATTAACTGAAACTGTCAAAGTGCAAGGCGAGCAGATTAGGGCTCAAGGAGAACAAATCAAAGCTCAGAGCGAAGAAATAAAAGAAATTAAGGTTGAGCAAAAAGCCCAAGGCGAGCAAATCAAAGAACTTCAAGTAGAACAAAAAGCCCAAGGTAAAACCCTACAGTTAATTTTAAAGACGCTAGAAAAAATGAACGAGCGTCTAGAAAAATTGGAATCTAAATAG", "aa": "MPKVISQKEFNILSRTKERVNFQIVYTKQRNGLHKAAKEYFFKDKDFTIIEDTPDTPDQPDGPCITKGPKEPGGPEEPNGPDKPEDPKDPDTPDVSEGPKEQGGQKDPGGPDDPSEGKQKVSKPDKYVTHRELDEKLKDFATKADFKRVEDKVDVLFELQKTQGEQIKVQGEQIKAQGKQIEQLTETVKVQGEQIRAQGEQIKAQSEEIKEIKVEQKAQGEQIKELQVEQKAQGKTLQLILKTLEKMNERLEKLESK", "aa_hexdigest": "1bb9cd6db02de9f774628c4ff99dd661", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000139F0E", "ncbi_nrp_id": "WP_010874370.1", "uniref100_id": "UniRef100_P75100", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139F0E", "RefSeq:WP_010874370.1", "UniRef:UniRef100_P75100" ] }, "ips": { "uniref100_id": "UniRef100_P75100", "uniref90_id": "UniRef90_P75100" }, "psc": { "uniref90_id": "UniRef90_P75100", "product": "UPF0134 protein MPN_013", "uniref50_id": "UniRef50_P75100" }, "pscc": { "uniref50_id": "UniRef50_P75100", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75100" ], "product": "UPF0134 protein MPN_013" }, "genes": [], "id": "APKECPOBEN_64", "locus": "GCA002090215_00021" }, { "type": "cds", "sequence": "contig_1", "start": 15867, "stop": 16439, "strand": "+", "frame": 3, "gene": "dnaG", "product": "putative MG010-like protein", "db_xrefs": [ "COG:COG0358", "COG:L", "GO:0003896", "GO:0005737", "SO:0001217", "UniRef:UniRef50_P75099", "UniRef:UniRef90_P75099" ], "nt": "ATGGAACTTAGAAATACAAAATTAGAACAACTCTTTTACATCTATCACCGCAACCTGAAGTATAGATGTCCAATGCAGTACCTCACTAAAAGGGGCTTTAACTTACAAGACCTCTTGTCAGTTGGTGGTGGATTAGCTTACTTGGGCGAAAAACAGTGGTTAAATTTATCACTGTACAACTTTAACAACCAGTTAGTTGGTTTTTTAAGTCGGAAGGTAGGTTTTGAAAAGAAGTTCCTTTATTTACCTATTAATAAACCACCAAGTAAAAGTGAATCTTTTTTAGGTTTAAAACACTTACCAACGGAAACTAACACCATTTACCTAGTTGAAGGTGATTTTGATTGGTTAGCTTTCCGTAAGGGTGGTATTTTGAACTGCTTGCCTTTATGTGGATTAACTTTATCAGATAAACAAATGAAGTTTTTTCAACAAAGTAAGATCGAAAAAGTGGTGCTTTGCTTAGACAATGATTTTGCTGGTAAAGTAGCTGCTGCTAACCTGGAACGCATTTTAAAAAATGCTGGTTTTCAGGTTAAGGTAGTTCAGCTAAAGGGTAAAGTTAAGGACTGA", "aa": "MELRNTKLEQLFYIYHRNLKYRCPMQYLTKRGFNLQDLLSVGGGLAYLGEKQWLNLSLYNFNNQLVGFLSRKVGFEKKFLYLPINKPPSKSESFLGLKHLPTETNTIYLVEGDFDWLAFRKGGILNCLPLCGLTLSDKQMKFFQQSKIEKVVLCLDNDFAGKVAAANLERILKNAGFQVKVVQLKGKVKD", "aa_hexdigest": "88f2bd83a79eaee97ec33021e7f80ae2", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75099", "query_cov": 1.0, "subject_cov": 0.8962264150943396, "identity": 1.0, "score": 389.0, "evalue": 9.48e-137, "valid": true, "gene": "DnaG", "product": "Uncharacterized protein MG010 homolog", "uniref50_id": "UniRef50_P75099", "cog_id": "COG0358", "cog_category": "L", "go_ids": [ "GO:0005737", "GO:0003896" ] }, "pscc": { "uniref50_id": "UniRef50_P75099", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75099" ], "product": "Uncharacterized protein MG010 homolog" }, "genes": [ "dnaG" ], "id": "APKECPOBEN_65", "locus": "GCA002090215_00022" }, { "type": "cds", "sequence": "contig_1", "start": 16482, "stop": 16619, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGTCAATGAAATCGAGGATGCTGCTGGTGCTAGGGCACTAACCAAAATGTGTCCTGACTTAAACCTACCAAGGGTAATTATTAAATCGTCCTTAACTCACTTTAAACATCATTTAAAGCGCCAAATGATCCCTTAA", "aa": "MVNEIEDAAGARALTKMCPDLNLPRVIIKSSLTHFKHHLKRQMIP", "aa_hexdigest": "7e76f1862693e124694bf8ee2d0e652a", "start_type": "GTG", "rbs_motif": "AATAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5125.0727, "isoelectric_point": 9.623147392272948 }, "id": "APKECPOBEN_66", "locus": "GCA002090215_00023" }, { "type": "cds", "sequence": "contig_1", "start": 16710, "stop": 17339, "strand": "-", "frame": 2, "gene": "lysX", "product": "putative MG011-like protein", "db_xrefs": [ "COG:COG0189", "COG:EHJQ", "GO:0005524", "GO:0005737", "GO:0009432", "GO:0018169", "GO:0046872", "SO:0001217", "UniRef:UniRef50_P47257", "UniRef:UniRef90_P75098" ], "nt": "ATGGCCAAAATTAAGTTAAAAAACAGAAAAGCACTCGTTGTTTACAATAAGACCGATTTTGACAAGAACAAGCACTTTGCTCAAGCACTAGTCGATGAACTGAACAAGAAAAAACTAGTTGGGCATATTTTGCTGTTAGATGATGAAACCGCTGATCACAAACACATTAAAAATGTGGAATTGATCATTAACCGTTCGCGCAGAATTGATTTTTTAACCAAACACAACTTTCTCAACAGTTTTCTCATTAATCCCCAAAATATCGTTTTGGTAGCCAATGATAAGTATGAAACTTACAGGTGGTTAAAGCAGCATAAGTTCTTGACCGTTGATACCACCATTTTTGATCCCAAAAAGATTAAGACTTTTCCGATTGTGATCAAAAAGCGTGATTCTTATGGTGGGGAAGATGTGCACTTAATTCAAAATGCGGAAGAAATTAAGCAGTTACCAATTCAAAATCCTAATGAGTGGATTGTCCAACCATTTTTGTCAATTGGTAAAGTGGAATACCGGGCTTACATCTTGTTTGGTAAGGTGTTAAAAACAATTAGAAGGACTGCTAGTGGTGATGATTTTAGGGCTAACTACTCACAAAATGCTGCTGTTGATTTGTTTAAACTAAAGTGA", "aa": "MAKIKLKNRKALVVYNKTDFDKNKHFAQALVDELNKKKLVGHILLLDDETADHKHIKNVELIINRSRRIDFLTKHNFLNSFLINPQNIVLVANDKYETYRWLKQHKFLTVDTTIFDPKKIKTFPIVIKKRDSYGGEDVHLIQNAEEIKQLPIQNPNEWIVQPFLSIGKVEYRAYILFGKVLKTIRRTASGDDFRANYSQNAAVDLFKLK", "aa_hexdigest": "0ab21d05bfb24ab38b794a6a63d4711c", "start_type": "ATG", "rbs_motif": null, "genes": [ "lysX" ], "truncated": "5-prime", "pseudogene": { "start": 16482, "stop": 17339, "observations": { "mutation": [ 16712 ], "stop": [ 16712 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75098", "query_cov": 1.0, "subject_cov": 0.7333333333333333, "identity": 1.0, "score": 420.0, "evalue": 1.79e-147, "gene_start": 1, "gene_end": 209, "reference_start": 1, "reference_end": 209, "reference_sequence": "MAKIKLKNRKALVVYNKTDFDKNKHFAQALVDELNKKKLVGHILLLDDETADHKHIKNVELIINRSRRIDFLTKHNFLNSFLINPQNIVLVANDKYETYRWLKQHKFLTVDTTIFDPKKIKTFPIVIKKRDSYGGEDVHLIQNAEEIKQLPIQNPNEWIVQPFLSIGKVEYRAYILFGKVLKTIRRTASGDDFRANYSQNAAVDLFKLKWYIKHKIKRIAKKLGHGYYAIDFFLNKYNRIVVNEIEDAAGARALTKMCPDLNLPRVIIKSSLTHFKHHLKRQMIP" }, "paralog": false, "identity": 0.9964912280701754, "score": 570.0, "evalue": 3.52e-209, "description": "Internal stop codon at 16712. Nonsense mutation around 16712.", "psc": { "uniref90_id": "UniRef90_P75098", "gene": "LysX", "product": "Uncharacterized protein MG011 homolog", "uniref50_id": "UniRef50_P47257", "cog_id": "COG0189", "cog_category": "EHJQ", "go_ids": [ "GO:0005737", "GO:0005524", "GO:0046872", "GO:0018169", "GO:0009432" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75098", "UniRef:UniRef50_P47257", "COG:COG0189", "COG:EHJQ", "GO:0005737", "GO:0005524", "GO:0046872", "GO:0018169", "GO:0009432" ] }, "pscc": { "uniref50_id": "UniRef50_P47257", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47257" ], "product": "Uncharacterized protein MG011" } }, "id": "APKECPOBEN_67", "locus": "GCA002090215_00024" }, { "type": "cds", "sequence": "contig_1", "start": 17339, "stop": 17827, "strand": "-", "frame": 3, "gene": "lysX", "product": "putative MG012-like protein", "db_xrefs": [ "COG:COG0189", "COG:EHJQ", "GO:0005524", "GO:0005737", "GO:0009432", "GO:0018169", "GO:0036211", "GO:0046872", "SO:0001217", "UniRef:UniRef50_P75097", "UniRef:UniRef90_P75097" ], "nt": "TTGGAACAATTCATCGAACAAATTAAGCGCTGTTTTCAGTTTCCAGTGATTGTCAAAAGTATCTATGGTTCTTTTGGTGATTACGTTTTTTTGTGCAAAGATGAACAGAAGTTACGTCAAACCTTAAGCGGGCTAACTGAACAGATCATTGTGCAGCAATACATTGCCACTTCTAATAGTGAAGCGGTCAGGGTAATTGTGGTCAATAACCAGGTAGTTGGGGCCTTACATACTCAAAATGAGGGTGATTTTCGCTCTAATTTAAATAAGGGAGCGGTTGGTGAACCATATCAACTAAGTCAGGAAGAAACCAAATTAGCAATTACAATTAGCCAAGCAATGCAACTCTTTTATTGTGGCATTGACTTTTTATTTGACCAGGATCGTTCGTTAATTTTCTGCGAAGTTAATTCCAATGTACAGCTCACCAAAAGCTCGATGTATTTGAAAACTAACCTCGCCATTCAACTTTTAGCATCAATCGCATAA", "aa": "MEQFIEQIKRCFQFPVIVKSIYGSFGDYVFLCKDEQKLRQTLSGLTEQIIVQQYIATSNSEAVRVIVVNNQVVGALHTQNEGDFRSNLNKGAVGEPYQLSQEETKLAITISQAMQLFYCGIDFLFDQDRSLIFCEVNSNVQLTKSSMYLKTNLAIQLLASIA", "aa_hexdigest": "da3b53ab4ef40bccb9279677950a0a35", "start_type": "TTG", "rbs_motif": null, "genes": [ "lysX" ], "truncated": "3-prime", "pseudogene": { "start": 17339, "stop": 18127, "observations": { "mutation": [ 17842 ], "stop": [ 17842 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75097", "query_cov": 1.0, "subject_cov": 0.5625, "identity": 0.9940000000000001, "score": 313.0, "evalue": 3.07e-106, "gene_start": 1, "gene_end": 162, "reference_start": 127, "reference_end": 288, "reference_sequence": "MKHISAVYNPAFTNIASKLNQTELLKDAAQSLNIQLDFFTCFDINTNQDKTKLPFKSNTILFLDKNIALAQWLESVGLRVINSSIAINNADNKALSHAVLAQHPTIKQIPTLIGPQNFRLAWYPEKLEQFIEQIKRCFQFPVIVKSIYGSFGDYVFLCKDEQKLRQTLSGLTEQIIVQQYIATSNSEAVRVIVVNNQVVGALHTQNEGDFRSNLNKGAVGEPYQLSQEETKLAITISQAMQLFYCGIDFLFDQDRSLIFCEVNSNVQLTKSSMYLKTNLAIQLLASIA" }, "paralog": false, "identity": 0.9961832061068703, "score": 510.0, "evalue": 3.62e-186, "description": "Internal stop codon at 17842. Nonsense mutation around 17842.", "psc": { "uniref90_id": "UniRef90_P75097", "gene": "LysX", "product": "Uncharacterized protein MG012 homolog", "uniref50_id": "UniRef50_P75097", "cog_id": "COG0189", "cog_category": "EHJQ", "go_ids": [ "GO:0005737", "GO:0005524", "GO:0046872", "GO:0018169", "GO:0036211", "GO:0009432" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75097", "UniRef:UniRef50_P75097", "COG:COG0189", "COG:EHJQ", "GO:0005737", "GO:0005524", "GO:0046872", "GO:0018169", "GO:0036211", "GO:0009432" ] }, "pscc": { "uniref50_id": "UniRef50_P75097", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75097" ], "product": "Uncharacterized protein MG012 homolog" } }, "id": "APKECPOBEN_68", "locus": "GCA002090215_00025" }, { "type": "cds", "sequence": "contig_1", "start": 17840, "stop": 18205, "strand": "-", "frame": 3, "gene": "lysX", "product": "putative MG012-like protein", "db_xrefs": [ "COG:COG0189", "COG:EHJQ", "GO:0005524", "GO:0005737", "GO:0009432", "GO:0018169", "GO:0036211", "GO:0046872", "SO:0001217", "UniRef:UniRef50_P75097", "UniRef:UniRef90_P75097" ], "nt": "ATGAAGCACATCAGCGCCGTATATAACCCTGCTTTTACTAACATTGCTTCCAAATTAAACCAAACGGAACTGTTAAAGGATGCAGCCCAGAGCTTAAACATCCAACTCGATTTCTTTACTTGCTTTGATATTAATACCAATCAGGATAAGACCAAACTACCCTTTAAGTCCAATACCATTTTGTTTTTGGATAAAAACATAGCGTTAGCGCAATGGTTAGAATCAGTTGGTTTGCGCGTTATTAACAGTTCGATTGCCATTAATAACGCGGACAATAAGGCCTTGAGTCATGCTGTTTTAGCGCAACACCCAACCATTAAACAGATTCCTACATTAATTGGCCCACAGAACTTTCGTTTAGCTTGA", "aa": "MKHISAVYNPAFTNIASKLNQTELLKDAAQSLNIQLDFFTCFDINTNQDKTKLPFKSNTILFLDKNIALAQWLESVGLRVINSSIAINNADNKALSHAVLAQHPTIKQIPTLIGPQNFRLA", "aa_hexdigest": "e3eadba98d3468ed554dbc7a60e2d616", "start_type": "ATG", "rbs_motif": "AAAA", "genes": [ "lysX" ], "truncated": "5-prime", "pseudogene": { "start": 17537, "stop": 18205, "observations": { "mutation": [ 17842 ], "stop": [ 17842 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75097", "query_cov": 1.0, "subject_cov": 0.4201388888888889, "identity": 1.0, "score": 241.0, "evalue": 3e-78, "gene_start": 1, "gene_end": 121, "reference_start": 1, "reference_end": 121, "reference_sequence": "MKHISAVYNPAFTNIASKLNQTELLKDAAQSLNIQLDFFTCFDINTNQDKTKLPFKSNTILFLDKNIALAQWLESVGLRVINSSIAINNADNKALSHAVLAQHPTIKQIPTLIGPQNFRLAWYPEKLEQFIEQIKRCFQFPVIVKSIYGSFGDYVFLCKDEQKLRQTLSGLTEQIIVQQYIATSNSEAVRVIVVNNQVVGALHTQNEGDFRSNLNKGAVGEPYQLSQEETKLAITISQAMQLFYCGIDFLFDQDRSLIFCEVNSNVQLTKSSMYLKTNLAIQLLASIA" }, "paralog": false, "identity": 0.9954954954954955, "score": 435.0, "evalue": 3.56e-157, "description": "Internal stop codon at 17842. Nonsense mutation around 17842.", "psc": { "uniref90_id": "UniRef90_P75097", "gene": "LysX", "product": "Uncharacterized protein MG012 homolog", "uniref50_id": "UniRef50_P75097", "cog_id": "COG0189", "cog_category": "EHJQ", "go_ids": [ "GO:0005737", "GO:0005524", "GO:0046872", "GO:0018169", "GO:0036211", "GO:0009432" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75097", "UniRef:UniRef50_P75097", "COG:COG0189", "COG:EHJQ", "GO:0005737", "GO:0005524", "GO:0046872", "GO:0018169", "GO:0036211", "GO:0009432" ] }, "pscc": { "uniref50_id": "UniRef50_P75097", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75097" ], "product": "Uncharacterized protein MG012 homolog" } }, "id": "APKECPOBEN_69", "locus": "GCA002090215_00026" }, { "type": "cds", "sequence": "contig_1", "start": 18273, "stop": 18887, "strand": "-", "frame": 2, "gene": "folD", "product": "Bifunctional protein FolD", "db_xrefs": [ "COG:COG0190", "COG:H", "GO:0000105", "GO:0004477", "GO:0004488", "GO:0005829", "GO:0006164", "GO:0009086", "GO:0035999", "SO:0001217", "UniRef:UniRef50_P47259", "UniRef:UniRef90_P75096" ], "nt": "GTGTCATCTGATGCCTTTATTCGCCAAAAAATGAATGCTTGTAATACTCTAGGGGCCCAGAGTGAACTAATTAAGTACCACGAAAGTGTCACTGAGAAAGAATTGTTAGAAAAAATTCAACAACTCAACACTGATCCTGAGGTAACAGGGATTATCTTGCAACTGCCAGTGTATCCCCATCTAAAACAAAACAAGCTCTTAAAAGCAATTGATCCTTACAAGGATGTGGATTACTTAACTGGTAACTGCCCCCTACCCATTAGGTCCTGTGTGGTGGAAGCGGTGTTAATTTTGAAAGAACACTTTCACCATGATTTCACCAACAAGCAGATTGTGGTGGTGGGTTTAGGCGTCACTGGTGGCGGGCCGATCTTTCACTATTTAAAGGAAACGGGTCATCAGGTGGTGGGATGTGACAAGCATACCCCCAACACCATGGAGCTAATTAAAACCGCTGACGTGGTGATCACAGCCATTGGCAAGCCCCATTTCTTTAAGACCACTAACTTTAAACCAGGTGTTATTCTTTATGATGTGGGGGTATCACGTAATGTCTTAAACAAACTGTGTGGTGACATCGATCCTAATGGGATAGAGAAAGTTGCCAAATGGTGA", "aa": "MSSDAFIRQKMNACNTLGAQSELIKYHESVTEKELLEKIQQLNTDPEVTGIILQLPVYPHLKQNKLLKAIDPYKDVDYLTGNCPLPIRSCVVEAVLILKEHFHHDFTNKQIVVVGLGVTGGGPIFHYLKETGHQVVGCDKHTPNTMELIKTADVVITAIGKPHFFKTTNFKPGVILYDVGVSRNVLNKLCGDIDPNGIEKVAKW", "aa_hexdigest": "1721000f47f651225fb5282494583a8e", "start_type": "GTG", "rbs_motif": null, "genes": [ "folD" ], "truncated": "both", "pseudogene": { "start": 18180, "stop": 18989, "observations": { "mutation": [ 18215, 18275, 18929 ], "stop": [ 18215, 18275, 18929 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75096", "query_cov": 1.0, "subject_cov": 0.758364312267658, "identity": 0.995, "score": 417.0, "evalue": 6.78e-147, "gene_start": 1, "gene_end": 204, "reference_start": 35, "reference_end": 238, "reference_sequence": "MSFDGKQLAHEILLTYKNRDWSQVKLVILQTNNDVSSDAFIRQKMNACNTLGAQSELIKYHESVTEKELLEKIQQLNTDPEVTGIILQLPVYPHLKQNKLLKAIDPYKDVDYLTGNCPLPIRSCVVEAVLILKEHFHHDFTNKQIVVVGLGVTGGGPIFHYLKETGHQVVGCDKHTPNTMELIKTADVVITAIGKPHFFKTTNFKPGVILYDVGVSRNVLNKLCGDIDPNGIEKVAKWWSPTPGGVGPFTVLAIMKNLWVLYEAHQRRI" }, "paralog": false, "identity": 0.9888475836431226, "score": 537.0, "evalue": 2e-196, "description": "Internal stop codon at 18215, 18275, 18929. Nonsense mutation around 18215, 18275, 18929.", "psc": { "uniref90_id": "UniRef90_P75096", "gene": "folD", "product": "Bifunctional protein FolD", "uniref50_id": "UniRef50_P47259", "cog_id": "COG0190", "cog_category": "H", "go_ids": [ "GO:0005829", "GO:0004477", "GO:0004488", "GO:0000105", "GO:0009086", "GO:0006164", "GO:0035999" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75096", "UniRef:UniRef50_P47259", "COG:COG0190", "COG:H", "GO:0005829", "GO:0004477", "GO:0004488", "GO:0000105", "GO:0009086", "GO:0006164", "GO:0035999" ] }, "pscc": { "uniref50_id": "UniRef50_P47259", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47259" ], "product": "Bifunctional protein FolD" } }, "id": "APKECPOBEN_70", "locus": "GCA002090215_00027" }, { "type": "tRNA", "sequence": "contig_1", "start": 19074, "stop": 19149, "strand": "-", "gene": "trnA", "product": "tRNA-Ala(tgc)", "amino_acid": "Ala", "anti_codon": "tgc", "score": 68.8, "nt": "GGGGATGTAGCTCAACTGATAGAGCACCTGATTTGCACTCAGGAGGTTGAGGGTTTGAGACCCTTCATCTCCACCA", "db_xrefs": [ "SO:0000254" ], "anti_codon_pos": [ 19114, 19116 ], "id": "APKECPOBEN_37", "locus": "GCA002090215_00028" }, { "type": "tRNA", "sequence": "contig_1", "start": 19155, "stop": 19231, "strand": "-", "gene": "trnI", "product": "tRNA-Ile(gat)", "amino_acid": "Ile", "anti_codon": "gat", "score": 84.3, "nt": "GGAAGTATAGCTCAGTTGGTTAGAGCACACCCCTGATAAGGGTGAGGTCGATGGTTCAAGTCCATTTACTTCCACCA", "db_xrefs": [ "SO:0000263" ], "anti_codon_pos": [ 19195, 19197 ], "id": "APKECPOBEN_36", "locus": "GCA002090215_00029" }, { "type": "cds", "sequence": "contig_1", "start": 19325, "stop": 20080, "strand": "+", "frame": 2, "gene": "mdlB", "product": "Putative ABC transporter ATP-binding MG014-like protein", "db_xrefs": [ "COG:COG1132", "COG:V", "GO:0005524", "GO:0005886", "GO:0015421", "GO:0016887", "SO:0001217", "UniRef:UniRef50_P47260", "UniRef:UniRef90_P75095" ], "nt": "ATGGGTCTTGTTTTAAAACAATTTAACAGGAAAATAAGAACAGCTTTAATTCTGGCGCCGCTTTTCACCTTTGCACAGATTATTATTGATCTTATTATTCCGTCCTTTTTGGCCAGTGCTATTGCCGTAGTGTTCTCAATTGTCACGCTTAAACAAAAAGAAGCATCAGGCGAGGGAGTTGCTGTTGATTTTATTGCTGAAAGTAAGTTAAGTTTCCAAAGTGTCCAGGAAGCGCAAATAGTGTTAGCGACCTCAGTGATCTTGTTAGCACTCTTTGGTTTAGTGTTTGGGTTAATTTCCATTTTTTGTGCCAGCATAGTAGCGGGGAATACCTCTTACTTTTTAAGAAGGAAGATTTTTCGCAAGATTATGCACATTACCGCACCTTCCCATGACCAATATGGCTCTTCTACGTTGTTGGTAAGGTTGACCAATGACGTTTACTTAATGGAAATCATGACCTTTGATTTTTTGCGGTTAATTGTTCGTGCACCTTTCTTGTTTATAGGGGGTCTAGCCTTTGCTATTGCCACTAACTCTGACATGTCGATTTCGCTAGCCATTACCTTTCCTTTGACCTTCTTAGTGATTGGGATTCTCAACAAGAAATCGACCCCGTTATTTAAGAACAACCAGAAATCGGTGGACCAAATTAACGAACGCGTGGAGGAAGATGTGTCGGGTTACAAGGTAGTGCAGTCGTTTAACTTAAAGGACACGGAATGTCTCAAGTTTAAGGCAGCTAATGCAAGGTGA", "aa": "MGLVLKQFNRKIRTALILAPLFTFAQIIIDLIIPSFLASAIAVVFSIVTLKQKEASGEGVAVDFIAESKLSFQSVQEAQIVLATSVILLALFGLVFGLISIFCASIVAGNTSYFLRRKIFRKIMHITAPSHDQYGSSTLLVRLTNDVYLMEIMTFDFLRLIVRAPFLFIGGLAFAIATNSDMSISLAITFPLTFLVIGILNKKSTPLFKNNQKSVDQINERVEEDVSGYKVVQSFNLKDTECLKFKAANAR", "aa_hexdigest": "b14ef64c41b6695270d5f377985cf1b4", "start_type": "ATG", "rbs_motif": null, "genes": [ "mdlB" ], "truncated": "3-prime", "pseudogene": { "start": 19325, "stop": 20383, "observations": { "mutation": [ 20078 ], "stop": [ 20078 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75095", "query_cov": 1.0, "subject_cov": 0.4028892455858748, "identity": 1.0, "score": 454.0, "evalue": 1.78e-155, "gene_start": 1, "gene_end": 251, "reference_start": 1, "reference_end": 251, "reference_sequence": "MGLVLKQFNRKIRTALILAPLFTFAQIIIDLIIPSFLASAIAVVFSIVTLKQKEASGEGVAVDFIAESKLSFQSVQEAQIVLATSVILLALFGLVFGLISIFCASIVAGNTSYFLRRKIFRKIMHITAPSHDQYGSSTLLVRLTNDVYLMEIMTFDFLRLIVRAPFLFIGGLAFAIATNSDMSISLAITFPLTFLVIGILNKKSTPLFKNNQKSVDQINERVEEDVSGYKVVQSFNLKDTECLKFKAANARWTKSSTNSLFVNTLNIPFTFFFSSMTIIIALLLVFQLDNSVRVDPLPDNAAIRPSIFAFFQYNFYIVLGLILTSLTMVNFTRSRVALGRIKDVLNKPEIQQHVVPDQTVLAPSLEFKNVAFGLGNKENRDFLQDLNFKFEAGKTYGIVGPTGSGKSLIANIIGGLYEPNQGEIFVGGQSIKTIDSDYLAKMIGIVFQQNILFKGTIASNIKIGLETREDWKHEPDSKKDAAMKRAAAIACADTFIEKFSDTYDHTVEQLGKNLSGGQKQRVAIARTVITKPQILVLDDSMSALDALTEKKVRENIANELPGTTKIIISQNINSIKYAHKIMVIDNGRIAGFDSDAKLMQSCDIYVKMKQAQKDQGGDFDAVA" }, "paralog": false, "identity": 0.9971590909090909, "score": 662.0, "evalue": 1.63e-239, "description": "Internal stop codon at 20078. Nonsense mutation around 20078.", "psc": { "uniref90_id": "UniRef90_P75095", "gene": "MdlB", "product": "Putative ABC transporter ATP-binding protein MG014 homolog", "uniref50_id": "UniRef50_P47260", "cog_id": "COG1132", "cog_category": "V", "go_ids": [ "GO:0005886", "GO:0015421", "GO:0005524", "GO:0016887" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75095", "UniRef:UniRef50_P47260", "COG:COG1132", "COG:V", "GO:0005886", "GO:0015421", "GO:0005524", "GO:0016887" ] }, "pscc": { "uniref50_id": "UniRef50_P47260", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47260" ], "product": "Putative ABC transporter ATP-binding protein MG014" } }, "id": "APKECPOBEN_71", "locus": "GCA002090215_00030" }, { "type": "cds", "sequence": "contig_1", "start": 20102, "stop": 21196, "strand": "+", "frame": 2, "gene": "mdlB", "product": "Putative ABC transporter ATP-binding MG014-like protein", "db_xrefs": [ "COG:COG1132", "COG:V", "GO:0005524", "GO:0005886", "GO:0015421", "GO:0016887", "SO:0001217", "UniRef:UniRef50_P47260", "UniRef:UniRef90_P75095" ], "nt": "TTGTTTGTCAATACACTGAACATTCCCTTTACCTTCTTCTTTTCCAGTATGACTATCATCATTGCGTTGCTTTTAGTCTTCCAACTAGATAATTCAGTCAGAGTGGATCCCCTACCAGATAATGCTGCCATTAGACCCAGCATCTTTGCTTTCTTCCAGTACAACTTCTATATTGTCTTGGGTTTAATCTTGACCTCGCTAACAATGGTCAACTTTACCCGTTCCCGCGTGGCCTTGGGTCGGATTAAGGATGTTTTGAATAAACCGGAAATCCAACAACATGTGGTTCCTGATCAAACAGTTCTAGCCCCTAGCTTGGAATTCAAAAACGTGGCCTTTGGGTTAGGTAACAAGGAAAACCGGGACTTCTTGCAAGACCTTAACTTTAAGTTTGAGGCCGGTAAGACTTACGGAATTGTGGGACCTACTGGTTCGGGTAAGTCTTTAATAGCTAATATTATTGGGGGTTTATATGAACCCAACCAAGGTGAAATCTTTGTTGGTGGTCAAAGTATTAAAACGATTGACAGTGACTATTTAGCCAAGATGATCGGGATTGTCTTTCAGCAAAACATTCTCTTTAAGGGCACGATTGCTTCCAACATTAAGATAGGCCTGGAAACCAGGGAAGACTGGAAGCACGAACCAGATAGCAAAAAGGATGCTGCCATGAAACGCGCTGCTGCCATTGCTTGTGCTGATACCTTCATCGAAAAGTTCAGCGATACCTATGACCATACGGTGGAACAGTTAGGCAAAAACCTCTCTGGTGGGCAAAAACAACGCGTCGCCATTGCTAGAACCGTTATTACTAAACCACAAATCTTAGTGTTGGATGACAGTATGAGTGCGCTGGACGCTTTAACCGAAAAGAAGGTCCGGGAAAACATTGCCAACGAACTTCCGGGCACCACTAAGATCATTATTAGTCAAAACATTAATTCGATTAAGTACGCGCACAAAATTATGGTGATTGATAACGGTCGGATTGCTGGGTTTGACAGTGATGCTAAGCTCATGCAGAGCTGTGACATCTATGTCAAGATGAAGCAAGCACAAAAAGACCAAGGAGGTGATTTCGATGCTGTCGCATAA", "aa": "MFVNTLNIPFTFFFSSMTIIIALLLVFQLDNSVRVDPLPDNAAIRPSIFAFFQYNFYIVLGLILTSLTMVNFTRSRVALGRIKDVLNKPEIQQHVVPDQTVLAPSLEFKNVAFGLGNKENRDFLQDLNFKFEAGKTYGIVGPTGSGKSLIANIIGGLYEPNQGEIFVGGQSIKTIDSDYLAKMIGIVFQQNILFKGTIASNIKIGLETREDWKHEPDSKKDAAMKRAAAIACADTFIEKFSDTYDHTVEQLGKNLSGGQKQRVAIARTVITKPQILVLDDSMSALDALTEKKVRENIANELPGTTKIIISQNINSIKYAHKIMVIDNGRIAGFDSDAKLMQSCDIYVKMKQAQKDQGGDFDAVA", "aa_hexdigest": "88b4702a464e77fe110fb5a9934f10dc", "start_type": "TTG", "rbs_motif": null, "genes": [ "mdlB" ], "truncated": "5-prime", "pseudogene": { "start": 19802, "stop": 21196, "observations": { "mutation": [ 20078 ], "stop": [ 20078 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75095", "query_cov": 1.0, "subject_cov": 0.5842696629213483, "identity": 0.997, "score": 704.0, "evalue": 7.8e-252, "gene_start": 1, "gene_end": 364, "reference_start": 260, "reference_end": 623, "reference_sequence": "MGLVLKQFNRKIRTALILAPLFTFAQIIIDLIIPSFLASAIAVVFSIVTLKQKEASGEGVAVDFIAESKLSFQSVQEAQIVLATSVILLALFGLVFGLISIFCASIVAGNTSYFLRRKIFRKIMHITAPSHDQYGSSTLLVRLTNDVYLMEIMTFDFLRLIVRAPFLFIGGLAFAIATNSDMSISLAITFPLTFLVIGILNKKSTPLFKNNQKSVDQINERVEEDVSGYKVVQSFNLKDTECLKFKAANARWTKSSTNSLFVNTLNIPFTFFFSSMTIIIALLLVFQLDNSVRVDPLPDNAAIRPSIFAFFQYNFYIVLGLILTSLTMVNFTRSRVALGRIKDVLNKPEIQQHVVPDQTVLAPSLEFKNVAFGLGNKENRDFLQDLNFKFEAGKTYGIVGPTGSGKSLIANIIGGLYEPNQGEIFVGGQSIKTIDSDYLAKMIGIVFQQNILFKGTIASNIKIGLETREDWKHEPDSKKDAAMKRAAAIACADTFIEKFSDTYDHTVEQLGKNLSGGQKQRVAIARTVITKPQILVLDDSMSALDALTEKKVRENIANELPGTTKIIISQNINSIKYAHKIMVIDNGRIAGFDSDAKLMQSCDIYVKMKQAQKDQGGDFDAVA" }, "paralog": false, "identity": 0.9978448275862069, "score": 899.0, "evalue": 0.0, "description": "Internal stop codon at 20078. Nonsense mutation around 20078.", "psc": { "uniref90_id": "UniRef90_P75095", "gene": "MdlB", "product": "Putative ABC transporter ATP-binding protein MG014 homolog", "uniref50_id": "UniRef50_P47260", "cog_id": "COG1132", "cog_category": "V", "go_ids": [ "GO:0005886", "GO:0015421", "GO:0005524", "GO:0016887" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75095", "UniRef:UniRef50_P47260", "COG:COG1132", "COG:V", "GO:0005886", "GO:0015421", "GO:0005524", "GO:0016887" ] }, "pscc": { "uniref50_id": "UniRef50_P47260", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47260" ], "product": "Putative ABC transporter ATP-binding protein MG014" } }, "id": "APKECPOBEN_72", "locus": "GCA002090215_00031" }, { "type": "cds", "sequence": "contig_1", "start": 21243, "stop": 21554, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCAAGGTCGCACTTCCTCCAAGGTGCTGTATGTCATGGTCTGTGCCATCTTTGGTATTCTCACTGGGGTTACTAACTCGATTCTCTTGGCCCAGGGCCTGGGTTTTATTTTTCCTACCACTAATACCGAAACTGATGGTATCCAATCAGTGTACTTACTGGTATTTGCGCACAACTTACCGGTCATGGAGCGCTTGACGATTGTTTGTGTTACCGTGGTTGTGGCTTACATTCTCATCTTTAGTTTTAACGTAGCGCAGAACTATTTAGGTTTAAAGCTGTACCAAGAGATCTGTGCCTTGTTGCGCTGA", "aa": "MQGRTSSKVLYVMVCAIFGILTGVTNSILLAQGLGFIFPTTNTETDGIQSVYLLVFAHNLPVMERLTIVCVTVVVAYILIFSFNVAQNYLGLKLYQEICALLR", "aa_hexdigest": "31c83c977d03b970aba79cd36d9158f8", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11365.43409999999, "isoelectric_point": 7.711539268493653 }, "id": "APKECPOBEN_73", "locus": "GCA002090215_00032" }, { "type": "cds", "sequence": "contig_1", "start": 21579, "stop": 22184, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCCACTAGTTTCTTTGATACGCAGAACAACGGGGATTTAATGAGTCGCTTGACCAACGACGTTTACAACATTAACAACCTGTATGCCCAAGTGGGTGGACAGACAATTCAGAGTTTGTTTATCTTAATGACAACAGCGACGATTCTGTTTGTTTTAAGTCCAGTCATTGCCTTAATTTCGCTAACAGTCTTAATTGCTTTAATTGCCCTTTCCTTTCTCTTTTTAAAGAAGGCACGCGCTGCTTATGCAAAGGTGCAAAACAACTTAGGGGACATGTCGGGTTACATTGAAGAAGTGTTGTCGAACCATAAGGTAGTTCATGTCTTGAAACTGCAAGAGGTGATGATTGATAACTTTGACAAGTACAACCGCCCGATGGTTAATCCCACCATTAAGGCCAATACCTATGCGGTGTTTATTTACTCCTGGTTTGGTTTTATTTCCAATATTACTTACTTAGCTTCGATCTCAATTGCCACGGCCTTTAGTGTGAATAACATTCCCTCCTTTGGGGTGAGTGCGATTAACTATTCGTTTATGCTGTCTTACATTGCGGCCTTACGTCAAACAGCACTCCCTTTAAACCAGATCTTTAGCTTGTGA", "aa": "MSTSFFDTQNNGDLMSRLTNDVYNINNLYAQVGGQTIQSLFILMTTATILFVLSPVIALISLTVLIALIALSFLFLKKARAAYAKVQNNLGDMSGYIEEVLSNHKVVHVLKLQEVMIDNFDKYNRPMVNPTIKANTYAVFIYSWFGFISNITYLASISIATAFSVNNIPSFGVSAINYSFMLSYIAALRQTALPLNQIFSL", "aa_hexdigest": "cbb029256f4e08b4f3171f348f58c13d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 22366.792800000003, "isoelectric_point": 8.803302955627444 }, "id": "APKECPOBEN_74", "locus": "GCA002090215_00033" }, { "type": "cds", "sequence": "contig_1", "start": 22233, "stop": 22514, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00005.33" ], "nt": "TTGGATCTAGAAAGTCCCCAAAAACAGGCCACCATTACTAAGTTACCCAACATTAAGGGCAACATCCGTTTTGAAAAGGTAGCTTTTGGTTACAGTGCGGACAAACCAATTTTAACGGGGATTGATTTCTCGGTTAAACACGGTGACATTGTGGCCATAGTCGGTCCTACTGGAGCGGGTAAGTCCACCATTATTAACCTGTTGATGAAGTTCTACAAACCGTTTGCTGGGAAGATCTACATGGACAACTTTGAGATTAGTGAGGTGAGTGAAACTGCTTGA", "aa": "MDLESPQKQATITKLPNIKGNIRFEKVAFGYSADKPILTGIDFSVKHGDIVAIVGPTGAGKSTIINLLMKFYKPFAGKIYMDNFEISEVSETA", "aa_hexdigest": "b9822d01461eb319aa53df4a7546dea7", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00005.33", "name": "ABC_tran", "length": 51, "aa_cov": 0.5483870967741935, "hmm_cov": 1.0, "evalue": 2.453782243145294e-14, "score": 49.54983139038086, "start": 38, "stop": 88 } ], "seq_stats": { "molecular_weight": 10167.684999999998, "isoelectric_point": 7.947614097595215 }, "id": "APKECPOBEN_75", "locus": "GCA002090215_00034" }, { "type": "cds", "sequence": "contig_1", "start": 22533, "stop": 23012, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00005.33" ], "nt": "GTGCTCCAAGACCCCTTCTTGTTTAGTGGCACGATTAAGGAAAACATCCGCATGGGACGGCAAGATGCCACTGATGAGGAAATTATAGAGGCGTGCAAGGTGGCCAATGCTCATGACTTTATTATGCGTTTACCCCAAGGTTACAACACCTTTATTTCCAACAAAACTGATTATCTCTCGGTCGGTGAGCGCCAACTGTTAACTATTGCCCGCGCTGTTATTCGTAATGCCCCGGTGTTATTATTGGATGAAGCCACGAGCTCCATCGATGTACACTCCGAAAAATTGATCCAACAATCAATAGGCAGACTAATGAAGGATAAAACATCGTTTATAATATCCCATCGACTCTCAATCATCCGTAATGCGACGCTGATAATTGTGATTAATGATGGAAAAGTTTTGGAAATGGGTAACCATGAACAATTAATGCGGCAAAACGGTTTTTATGCTCGATTAAAGCGCTCTGCTGTGAAGTAA", "aa": "MLQDPFLFSGTIKENIRMGRQDATDEEIIEACKVANAHDFIMRLPQGYNTFISNKTDYLSVGERQLLTIARAVIRNAPVLLLDEATSSIDVHSEKLIQQSIGRLMKDKTSFIISHRLSIIRNATLIIVINDGKVLEMGNHEQLMRQNGFYARLKRSAVK", "aa_hexdigest": "eec678936ab4730ac6829e1efe0efd98", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00307619A1", "query_cov": 0.9811320754716981, "subject_cov": 0.8082901554404145, "identity": 0.551, "score": 176.0, "evalue": 3.12e-53, "valid": false, "product": "ATP-binding cassette domain-containing protein", "uniref50_id": "UniRef50_A0A354MUK2" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00005.33", "name": "ABC_tran", "length": 88, "aa_cov": 0.5345911949685535, "hmm_cov": 0.8181818181818182, "evalue": 1.5558922110703375e-11, "score": 40.47453308105469, "start": 3, "stop": 87 } ], "seq_stats": { "molecular_weight": 18043.70329999999, "isoelectric_point": 9.12822360992432 }, "id": "APKECPOBEN_76", "locus": "GCA002090215_00035" }, { "type": "cds", "sequence": "contig_1", "start": 23022, "stop": 23396, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF04434.22" ], "nt": "ATGACGATTGCTGAAATAAGGAAATTTGCACAAACCACGCAAAAGTTTGTCGAAGCGGAAAACCTGTTTGAGCATGGTAACGTCGTCTTGCCCAAAAAATACCTCAATAAGGCGCGGGGCATGGCTGAAGTATTGTACAACAGCCAGGTCATTAAAGTAAGTTTTACGGCTAAAGATGGTGAGCTAACGTGTAAGTGTAGTTGTTTGGCTAACGTTGACAACTGCGTGCACATTGTAGCTGTATTGCTCAAATACCACCAAATGCTAGTGGAGTCCAAACGCAGTTTTAACTTAGCTGAAGCCTTTCACTTGGACTGTGATCAGGCCGAAATGCTGATTGAAAACCTCAGTTTAGAGATTATTGCCGGGGGATGA", "aa": "MTIAEIRKFAQTTQKFVEAENLFEHGNVVLPKKYLNKARGMAEVLYNSQVIKVSFTAKDGELTCKCSCLANVDNCVHIVAVLLKYHQMLVESKRSFNLAEAFHLDCDQAEMLIENLSLEIIAGG", "aa_hexdigest": "1c9ab90bbbc57fb82fa825fca0a254e2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF04434.22", "name": "SWIM", "length": 29, "aa_cov": 0.20967741935483872, "hmm_cov": 1.0, "evalue": 6.198287009431385e-08, "score": 27.563405990600586, "start": 60, "stop": 85 } ], "seq_stats": { "molecular_weight": 13894.024299999988, "isoelectric_point": 6.063549613952636 }, "id": "APKECPOBEN_77", "locus": "GCA002090215_00036" }, { "type": "cds", "sequence": "contig_1", "start": 23454, "stop": 24554, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCGCTTTTATTGTTGTGATGCGACCTTTGTGTACTTCCTTCACTTGGAAAACGACACTTTGCACTCAGTCGAATTGTCCAAGTTTAAACCAGAGGAACGCGCTACCTTACTCTTCTTTGATAAATTGTGCAAGCAGTTTACAGTGGGGTATGACCGCAACAGTTTGCTCTTTCCGTTAGCGGGTTTTTTAAAGGAGTTACAAGCCAACACGGAGCCCACCATCTTTGTCTTTAACGATGACAAAATTGACAACATCCTCTTTTTAAGGATTTCCAAAAAGCACCATGGTTTAAACCATGTTTGTGGTTTTAGTGGCAAGAAGGTATTTGACTTTGTTACTTATAAACAAAAGGAAAAGCAGATTGTGTTGCGCTCAGCGTACCTGAGTAAGTTCACTGACTTTAAGTTTCCTTACACCATTAACATCTACAAACTCCAGTTTGGGGAACCGCTGTTCTTTTACTTTTTAATTCAGCTAAAGCGTGATGGCTTTAAGAACTTCTACTTTCAAAGTGAAGACAGTATTGTCAAGGAAAAGGAATACCTACCGAAGCTGTACTTTAAGGTCGAGTACGATCCGGTCAAAAACAAGTTTGTCAGTGATGCCTTCTTTAAGTACAAGAACCACTTTAATAAGGGCACAACCACGGTGTATCCGCACCGCTATTACATGGCTAAAAAGTCGGATCGCGGTGGTTTTAACCGCTTGTTGTTTTACGAAGAGGCCGTGGAAAACTTCTACCAAGACCAGTTTGATTTGGGTTACTTCCGTAAGTTTGAACACCTACCGATCCAGGACAAAAACCAGATTGAGGCCTTTAAAGCAGCGCTAGATGACTTAATGCCCGTGGATTTGGCTGAAGTGAGTTTATCCGATAACTTACTCCACCAAAAACCGCTTCATTTTGCCCTGTCTGATCTAGAAGCAGTTGCTGTTGATGACAAACAAATTAAGTTAAGTTTTGCCCCTAGTGCGGTTGAACTGAAATTAATTAAACGGATCTTATCCGCTTACCATAAGGGCAATGTGGTCTGTATCGACCAGGAGAGTTGGTATGACCTCAAGCAACCAGCTGCTAAAGAACTAATCCAGTTCTGA", "aa": "MRFYCCDATFVYFLHLENDTLHSVELSKFKPEERATLLFFDKLCKQFTVGYDRNSLLFPLAGFLKELQANTEPTIFVFNDDKIDNILFLRISKKHHGLNHVCGFSGKKVFDFVTYKQKEKQIVLRSAYLSKFTDFKFPYTINIYKLQFGEPLFFYFLIQLKRDGFKNFYFQSEDSIVKEKEYLPKLYFKVEYDPVKNKFVSDAFFKYKNHFNKGTTTVYPHRYYMAKKSDRGGFNRLLFYEEAVENFYQDQFDLGYFRKFEHLPIQDKNQIEAFKAALDDLMPVDLAEVSLSDNLLHQKPLHFALSDLEAVAVDDKQIKLSFAPSAVELKLIKRILSAYHKGNVVCIDQESWYDLKQPAAKELIQF", "aa_hexdigest": "452b9fe59c4a5efad56200addd43075d", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 43283.27430000007, "isoelectric_point": 8.316888999938964 }, "id": "APKECPOBEN_78", "locus": "GCA002090215_00037" }, { "type": "cds", "sequence": "contig_1", "start": 24627, "stop": 24785, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGCCAAGATCTTTAGTCAGTACTTGGACATTAAAAACCTGTTTGATGTACCGACCATTAAAAAGATTGAAGACCAAAACAACAACGTCTTTGACCTTTCCTTAGAACACAAAAAGATTACTTCACTGCGCAACTACCAACAAGAAGGAGTGAAGTGA", "aa": "MAKIFSQYLDIKNLFDVPTIKKIEDQNNNVFDLSLEHKKITSLRNYQQEGVK", "aa_hexdigest": "7fcdafb0b2a1e95f22e7e76ad5f862b2", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6155.9847, "isoelectric_point": 8.029811286926268 }, "id": "APKECPOBEN_79", "locus": "GCA002090215_00038" }, { "type": "cds", "sequence": "contig_1", "start": 24795, "stop": 24947, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00176.29" ], "nt": "TTGGAGGAAAATAAGTTTGGTGGTATTCTGGCTGACGAAATGGGGTTAGGGAAAACGGTGCAGGTGATCTTTGCGCTGTTGGACAGCTATCTCAAAAACCACGTTAACCTCCCTAGCTTAATTATTGTTCCGGCTTCTTTATTGTTGAACTGA", "aa": "MEENKFGGILADEMGLGKTVQVIFALLDSYLKNHVNLPSLIIVPASLLLN", "aa_hexdigest": "06aeeb07d933bf4c611fa98f18b6cd66", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00176.29", "name": "SNF2-rel_dom", "length": 53, "aa_cov": 0.96, "hmm_cov": 0.9811320754716981, "evalue": 3.710502846478919e-13, "score": 44.63841247558594, "start": 2, "stop": 49 } ], "seq_stats": { "molecular_weight": 5440.376199999999, "isoelectric_point": 4.895849418640137 }, "id": "APKECPOBEN_80", "locus": "GCA002090215_00039" }, { "type": "cds", "sequence": "contig_1", "start": 25023, "stop": 25247, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00176.29" ], "nt": "TTGTACGAAAAACTTACTAACGAAATCCTGATTGTGAGCTTTAACGTGTTGCGCAGTGATGTGAAGTTAATTACCAAACAGCGCTTTCACTATGTTGTAATTGACGAAGCGCAGGGCATTAAAAACGACAGCTCTTCAATTACCAAAGCTGCCAAAAAGGTGAAGGGCAACTTCTGTTTGGCCTTAACTGGTACGCCAATAGAAAACCGCTTGTTGGACCTTTGA", "aa": "MYEKLTNEILIVSFNVLRSDVKLITKQRFHYVVIDEAQGIKNDSSSITKAAKKVKGNFCLALTGTPIENRLLDL", "aa_hexdigest": "2f074a5cf1e1038ab1f889e593fc6949", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00176.29", "name": "SNF2-rel_dom", "length": 69, "aa_cov": 0.9324324324324325, "hmm_cov": 1.0, "evalue": 4.1923303181648393e-17, "score": 57.59275817871094, "start": 6, "stop": 74 } ], "seq_stats": { "molecular_weight": 8353.668099999999, "isoelectric_point": 9.206359291076659 }, "id": "APKECPOBEN_81", "locus": "GCA002090215_00040" }, { "type": "cds", "sequence": "contig_1", "start": 25263, "stop": 25898, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00271.37", "PFAM:PF00176.29" ], "nt": "GTGCTCCCAAGCTTTTTAGGGAACAAAAAGCAGTTTACCGACCAGTTTGAAAAGGAAAAAACCGACCAAAGCTTCCATTTGTTAATGCAGCGCACTAGTCCCTTTATTTTGCGTCGTACCAAGTCCAAGGTCTTGAAGGAACTGCCTAACAAAATTACCACCGATATTTACGTAGAATTAAACCCAATGCACCAGAAGCTGTATGAGGAAGAACGTGACCGCGGTTTGGAGGAAATTAAGCAAATCCAAGACAAGAGTTCCTTTAACATCTTGACTTTAATCTTGAAGTTAAGGCACTTGTGTAGTTTACCCAAAAACAGTCAGGGTATTTTGGAAAACAGTGCCAAAAAGGAAGCTGCTTTAGAAATTATCCACGAAGCGATTGAAAACCAGCGCAAGATTATCCTCTTTACCCAGTTCATTGATGTAATTGACCACTTTAAAGACACCTTTAAAGAGCAGGGCATTGAGTACTTTATCTTTGATGGCCGTAAGTCTCCTAAAAGCCGTCACAGTATTATCGAGAAGTTCAACAATGCCAAAAACCCTTGTGTGTTGTTAGCGTCCTTGAAAGCAGGGGGTGTGGGAATTAACCTCACTGCCGCTGAAGTGGTAATCCACTTTGATGTGTGGTGA", "aa": "MLPSFLGNKKQFTDQFEKEKTDQSFHLLMQRTSPFILRRTKSKVLKELPNKITTDIYVELNPMHQKLYEEERDRGLEEIKQIQDKSSFNILTLILKLRHLCSLPKNSQGILENSAKKEAALEIIHEAIENQRKIILFTQFIDVIDHFKDTFKEQGIEYFIFDGRKSPKSRHSIIEKFNNAKNPCVLLASLKAGGVGINLTAAEVVIHFDVW", "aa_hexdigest": "fbae1f9d8d0b765c8039e2539f9645fd", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00271.37", "name": "Helicase_C", "length": 94, "aa_cov": 0.44075829383886256, "hmm_cov": 0.9468085106382979, "evalue": 1.14420083630995e-18, "score": 62.93749237060547, "start": 119, "stop": 211 }, { "id": "PF00176.29", "name": "SNF2-rel_dom", "length": 109, "aa_cov": 0.4834123222748815, "hmm_cov": 1.0, "evalue": 3.909460523643263e-14, "score": 47.84605407714844, "start": 3, "stop": 104 } ], "seq_stats": { "molecular_weight": 24498.081100000018, "isoelectric_point": 8.939589118957521 }, "id": "APKECPOBEN_82", "locus": "GCA002090215_00041" }, { "type": "cds", "sequence": "contig_1", "start": 25908, "stop": 26114, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGAAAACCAAGCGACTGACCGGGCCCACCGCATAGGACAAAAGAAAACAGTCCAAGTTTACCGCATTATTGCTAAAAATACGATTGAAGAGCGGGTCTGTCAAGTGCAGGCCGAAAAACAGGAGTTAGTGAGCAAAACGCTAGTGGAAGACGTCAATTTTTTCGAATCCTTGACCAATGAGGAATTATTACGTCTTTTTGAATAA", "aa": "MENQATDRAHRIGQKKTVQVYRIIAKNTIEERVCQVQAEKQELVSKTLVEDVNFFESLTNEELLRLFE", "aa_hexdigest": "541de4aabdea3cbc664f5d836f4aae02", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8009.008499999999, "isoelectric_point": 5.4145620346069325 }, "id": "APKECPOBEN_83", "locus": "GCA002090215_00042" }, { "type": "cds", "sequence": "contig_1", "start": 26160, "stop": 27332, "strand": "+", "frame": 3, "gene": "dnaJ", "product": "Chaperone protein DnaJ", "db_xrefs": [ "COG:COG0484", "COG:O", "GO:0005524", "GO:0005737", "GO:0006260", "GO:0008270", "GO:0009408", "GO:0031072", "GO:0042026", "GO:0051082", "GO:0051085", "RefSeq:WP_010874378.1", "SO:0001217", "UniParc:UPI000012958B", "UniRef:UniRef100_P78004", "UniRef:UniRef50_P78004", "UniRef:UniRef90_P78004" ], "nt": "ATGGCCGCTGGTAAACGTGATTATTATGAAGTGTTAGGCGTTTCCAGATCCGCCACCGCACAAGACATCAAACGAGCTTTCCGCAAGCTTGCTATGCAGTACCACCCCGACCGCCATAAGGGTGAGGGTGAGACTGTCCAAAAACAAAACGAAGAGAAGTTTAAAGAGGTCAATGAGGCGTACGAGGTCTTAAGTGACACCGAGAAGCGTGGTATGTACGACCGCTTTGGCCATGAAGGGTTAAATGCCTCTGGCTTCCATGAAACTGGGTTTAATCCCTTTGACATCTTTAACAGTGTCTTTGGTGAGGGCTTTTCGTTTGACATGGACGGGGGTTCACCGTTTGACTTTATCTTTAGTAGGGGCAAAAAGTCGCGGCAAAACCGGGTTGTTTTGCCCTATGATTTAGAGATTGCGGTGGGGGTAGACATTAGCTTCTTTGAGATGACTAATGGTTGTACTCGGACGATTGAATATACCAAACGGGTGACCTGTAGTGCCTGTGATGGTTTTGGGGCTGAAGGGGGAGAAACCGGGATGGTTAGCTGTAACAGTTGTGAAGGCAATGGTTTTATTTTGAAGAATCAACGCTCCATCTTTGGTACAGTCCAGTCCCAGATGTTGTGTCAGAGCTGTGGTGGTCAGGGTAAACAGGCCAAACACAAGTGTAAGACTTGTAAGGGAAGTAAGTACAAGAAGGTACCAACTACCAAGGAAATTCAGATTCCCGCTGGCATTTACCATGGTGATGCGTTAGTCGATGACCATGGTGGTAATGAATTCGGGGGTCACGTTGGCAAGTTAGTGGTCCATGTGGGGGTACTACCGAGTAAGATCTTTAAACGGGTTGACCATAATGTAGTAGCCAATGTTTTAGTCGATCCGATGATTGCTATCACCGGAGGGAAAATTGAACTCCCTACCTTAGAAGGCATTAAGGAATTCAACTTACGTGCTGGTACTAAGAGTGGCGAGCAAATAGTAATCCCTGGTGGTGGGATTAAATTTACGAAGAACTTCCGCAAGAAGGCCGGGGATCTCATTATTATTATCAGTTATGCCCGTCCAAGTGAATACAGTGCGCCAGAGTTGCGCAAGCTCAAGGAATTTATTAAACCTAATCAAGAGGTAAAACAATATTTAAACGCACTTAAGAATGAATACAAGTCCTAA", "aa": "MAAGKRDYYEVLGVSRSATAQDIKRAFRKLAMQYHPDRHKGEGETVQKQNEEKFKEVNEAYEVLSDTEKRGMYDRFGHEGLNASGFHETGFNPFDIFNSVFGEGFSFDMDGGSPFDFIFSRGKKSRQNRVVLPYDLEIAVGVDISFFEMTNGCTRTIEYTKRVTCSACDGFGAEGGETGMVSCNSCEGNGFILKNQRSIFGTVQSQMLCQSCGGQGKQAKHKCKTCKGSKYKKVPTTKEIQIPAGIYHGDALVDDHGGNEFGGHVGKLVVHVGVLPSKIFKRVDHNVVANVLVDPMIAITGGKIELPTLEGIKEFNLRAGTKSGEQIVIPGGGIKFTKNFRKKAGDLIIIISYARPSEYSAPELRKLKEFIKPNQEVKQYLNALKNEYKS", "aa_hexdigest": "c15a02691b10973fc889082929c5eaf5", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000012958B", "ncbi_nrp_id": "WP_010874378.1", "uniref100_id": "UniRef100_P78004", "db_xrefs": [ "SO:0001217", "UniParc:UPI000012958B", "RefSeq:WP_010874378.1", "UniRef:UniRef100_P78004" ] }, "ips": { "uniref100_id": "UniRef100_P78004", "uniref90_id": "UniRef90_P78004" }, "psc": { "uniref90_id": "UniRef90_P78004", "gene": "dnaJ", "product": "Chaperone protein DnaJ", "uniref50_id": "UniRef50_P78004", "cog_id": "COG0484", "cog_category": "O", "go_ids": [ "GO:0005737", "GO:0005524", "GO:0031072", "GO:0051082", "GO:0008270", "GO:0051085", "GO:0006260", "GO:0042026", "GO:0009408" ] }, "pscc": { "uniref50_id": "UniRef50_P78004", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P78004" ], "product": "Chaperone protein DnaJ" }, "genes": [ "dnaJ" ], "id": "APKECPOBEN_84", "locus": "GCA002090215_00043" }, { "type": "cds", "sequence": "contig_1", "start": 27493, "stop": 28245, "strand": "+", "frame": 1, "gene": "pip", "product": "prolyl aminopeptidase", "db_xrefs": [ "COG:COG0596", "COG:HR", "EC:3.4.11.5", "GO:0004177", "GO:0005737", "GO:0006508", "GO:0030163", "SO:0001217", "UniRef:UniRef50_P75092", "UniRef:UniRef90_P75092" ], "nt": "TTGTTAGACCAACGGGGTTGTGGTCAAAGTAAAGCGGTTAATCCCTTACTGCACAACACTACCCAAGACTTAGTTGGGGACTTGGAAGCGTTGCGCCAACACTTAAAACTTGAGCGTTGGACCCTATTTGGTGGTAGTTGGGGTTCTACTTTAGCCTTAGTGTATGCCATTACCCATCCCCAAGTAGTCGAACAAGTCTTTTTAAGAGCGCTCTTTTTGGGCCGTGAACAAGACTGGGCTGAAATGCTGTTGGGCTTGGGCAAGCTCTTCTACCCTTACGAACACCAAACGTTACTGAAAGCCATTCCCCAGGCTTGTCGCACTGACTTTACGAAATTCACTAACTACTTTTATGAAGTGTTGCAAGGTAACGATTCCGCTTTAAAAACGCAGTTAGCCAACGCTTGGGTTAAGTGGGAAAATACCTTACTATCACCGATTAGCTATGTCAAAGATGAGAAAGCAGAAGATGCGAACTTTACCTTTAAGTTAGCGCTCTTGGAATGTCACTACGCAAAGCACCACAGCTTTTTAAAACCGAACTTTATCCTGGAAAATGTTGCGGTATTAAAGGACAAACCGGTGCACTTAATCCACGGCCGTTTTGATCTAGTCTGTCCGTTAAGTCAAGCCTTGGAACTCAAACGTGCTTTACCAACGCTTAACTTGTATGTAACTAACAATGCCGGGCACAGTGGCAGTGATCCCAATAACCTCACTACCATTAAACACCTTTTAAAAACACAACTGTAA", "aa": "MLDQRGCGQSKAVNPLLHNTTQDLVGDLEALRQHLKLERWTLFGGSWGSTLALVYAITHPQVVEQVFLRALFLGREQDWAEMLLGLGKLFYPYEHQTLLKAIPQACRTDFTKFTNYFYEVLQGNDSALKTQLANAWVKWENTLLSPISYVKDEKAEDANFTFKLALLECHYAKHHSFLKPNFILENVAVLKDKPVHLIHGRFDLVCPLSQALELKRALPTLNLYVTNNAGHSGSDPNNLTTIKHLLKTQL", "aa_hexdigest": "c9361cf0dd788333a54a3bbd304fb246", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75092", "query_cov": 1.0, "subject_cov": 0.8090614886731392, "identity": 0.996, "score": 513.0, "evalue": 2.13e-183, "valid": true, "gene": "pip", "product": "prolyl aminopeptidase", "ec_ids": [ "3.4.11.5" ], "uniref50_id": "UniRef50_P75092", "cog_id": "COG0596", "cog_category": "HR", "go_ids": [ "GO:0004177", "GO:0005737", "GO:0006508", "GO:0030163" ] }, "pscc": { "uniref50_id": "UniRef50_P75092", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75092" ], "product": "Putative proline iminopeptidase" }, "genes": [ "pip" ], "id": "APKECPOBEN_85", "locus": "GCA002090215_00044" }, { "type": "cds", "sequence": "contig_1", "start": 28245, "stop": 28487, "strand": "+", "frame": 3, "gene": null, "product": "Methionine--tRNA ligase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2M6W2H4" ], "nt": "ATGAAACGGTGTTACATTACCACCCCGATTTACTACGCCTCGGGTAAGCCCCACATTGGCCATGCCTTTACGACCATCTTAGCGGATGTGATTAAGCGGTATAAACAGCAAAACGGTTATGAAGCTTACTTTCTAACTGGTACGGATGAACACGGCAACAAAATTGAAAGTAAGGCCAAAAGTTTAGGTCTAGATCCCCAAACCTTTGTGGATCAAAACGTGGCTTACTTTCAACAAATGTGA", "aa": "MKRCYITTPIYYASGKPHIGHAFTTILADVIKRYKQQNGYEAYFLTGTDEHGNKIESKAKSLGLDPQTFVDQNVAYFQQM", "aa_hexdigest": "e42754332f3ed166bcbd7561bddc9cc2", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI003620B786", "query_cov": 0.8375, "subject_cov": 0.9054054054054054, "identity": 0.5820000000000001, "score": 90.5, "evalue": 2.34e-22, "valid": false, "product": "class I tRNA ligase family protein", "uniref50_id": "UniRef50_A0A2M6W2H4" }, "pscc": { "uniref50_id": "UniRef50_A0A2M6W2H4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2M6W2H4" ], "product": "Methionine--tRNA ligase" }, "genes": [], "id": "APKECPOBEN_86", "locus": "GCA002090215_00045" }, { "type": "cds", "sequence": "contig_1", "start": 28665, "stop": 29063, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGTGTGAAATAGGTCACCAGTTAACCCTAGTCCAAGAACCATCTTTATTTATTGCCTTTAAAGATAGTAAGGACTGGATTGGGGAAATGATTGCGACAAACAAGCTCAACATTACCCCTGAATCCAGAGCCGCTGAGTTAAAGAACAACTTCTTGGATGGGGGTTTAAATGATTTAGCTTTAACACGGCAAAACGTGACTTGGGGAATTCCCGTACCCTTTGACAACAAGCAAACGATTTACGTGTGGTTTGATGCCTTGTTTAACTACATTACTAACTTAGGGTTTGCCCATAACGACCCTAAATTTAACAAATGGTGGAACAATAATGATGAGGAACACGAAGTCATTCATTTAATTTCACGCGAAATTACCCGTTTCCACTGCATTTACTGA", "aa": "MLCEIGHQLTLVQEPSLFIAFKDSKDWIGEMIATNKLNITPESRAAELKNNFLDGGLNDLALTRQNVTWGIPVPFDNKQTIYVWFDALFNYITNLGFAHNDPKFNKWWNNNDEEHEVIHLISREITRFHCIY", "aa_hexdigest": "6c8fca86a153831eb582f9744a053720", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15439.314699999992, "isoelectric_point": 5.234893989562988 }, "id": "APKECPOBEN_87", "locus": "GCA002090215_00046" }, { "type": "cds", "sequence": "contig_1", "start": 29082, "stop": 29456, "strand": "+", "frame": 3, "gene": null, "product": "class I tRNA ligase family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0013310053", "UniRef:UniRef90_UPI0013310053" ], "nt": "TTGGGTTTTAAACTACCGACCCAGTTTCTCTCCCATGGTTGGATTGTCGATGGTAATGGGCACAAGATGTCCAAGTCGTTGGGCAATGTCATTTCCCCCGAAGAACTATTGGCCCAGTTTGGTGTTGATGGTACGCGTTATTGCTTACTTAAAGAAATGCGCTTGGACAAGGACAACCGCTGTAGTATGGCGATCTTTAAGGACATTTACAACGCTGATTTGGCCAACAGTTTTGGTAACCACGCATCACGTACCTTTGGCATGATCAAAAAGTACTTGGGCGGCCAGTTAGACTTTATCGAAGTCCAAGACCCTCAAGTAAAGCAGCTAATGGACCAAGCGAACCAAGCGATGGTTCAGTTTGACACCGCTTGA", "aa": "MGFKLPTQFLSHGWIVDGNGHKMSKSLGNVISPEELLAQFGVDGTRYCLLKEMRLDKDNRCSMAIFKDIYNADLANSFGNHASRTFGMIKKYLGGQLDFIEVQDPQVKQLMDQANQAMVQFDTA", "aa_hexdigest": "a1cc41a251b5c8eb0417ddd3013ffeb7", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 28998, "stop": 29759, "observations": { "mutation": [ 29061, 29454 ], "stop": [ 29061, 29454 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0013310053", "query_cov": 1.0, "subject_cov": 0.47509578544061304, "identity": 0.9840000000000001, "score": 251.0, "evalue": 1.14e-82, "gene_start": 1, "gene_end": 124, "reference_start": 29, "reference_end": 152, "reference_sequence": "DEEHEVIHLISREITRFLCIYWPIFLHQLGFKLPTQFLSHGWIVDGNGHKMSKSLGNVISPEELLAQFGVDGTRYCLLKEMRLDKDNRCSMAIFKDIYNADLANSFGNHASRTFGMIKKYLGGQLDFIEVQDPQVKQLMDQADQAMVQFDTAWNNFQFYKGINGLLQLVFQASKLIDQLKPWELVKQTDYTLLKQLLFACVRCTQVCFVLLAPILVHTSTQIFDLFNFSAQARSKTHLADPQQLQKISLAPVIQLLFKRLD" }, "paralog": false, "identity": 0.9841897233201581, "score": 500.0, "evalue": 2.79e-183, "description": "Internal stop codon at 29061, 29454. Nonsense mutation around 29061, 29454.", "psc": { "uniref90_id": "UniRef90_UPI0013310053", "product": "class I tRNA ligase family protein", "uniref50_id": "UniRef50_UPI0013310053", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0013310053", "UniRef:UniRef50_UPI0013310053" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0013310053", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0013310053" ], "product": "class I tRNA ligase family protein" } }, "id": "APKECPOBEN_88", "locus": "GCA002090215_00047" }, { "type": "cds", "sequence": "contig_1", "start": 29613, "stop": 29783, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTGCTTTGTCTTGTTAGCGCCGATCTTAGTTCATACCAGTACCCAGATATTTGACCTGTTTAATTTCAGTGCCCAAGCACGTAGTAAGACCCACCTAGCTGATCCACAACAACTGCAAAAAATCAGCTTAGCACCTGTAATTCAACCTTTATTTAAAAGATTGGATTAA", "aa": "MCFVLLAPILVHTSTQIFDLFNFSAQARSKTHLADPQQLQKISLAPVIQPLFKRLD", "aa_hexdigest": "f736ee52ad9085f969f6255934fe799b", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6351.4431, "isoelectric_point": 9.186051750183104 }, "id": "APKECPOBEN_89", "locus": "GCA002090215_00048" }, { "type": "cds", "sequence": "contig_1", "start": 29804, "stop": 29902, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACTATGCAAGTAGAATACCTCGATCTCATCTCCCAAGCGAAGGAGATTGCTGAGACCCAATTTAAGGCGGAACCGTTTTCCTTTGATGCTATTTGA", "aa": "MTMQVEYLDLISQAKEIAETQFKAEPFSFDAI", "aa_hexdigest": "6974cd282a195b65a38f65d1f70d5bb3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3695.1731, "isoelectric_point": 4.07884578704834 }, "id": "APKECPOBEN_90", "locus": "GCA002090215_00049" }, { "type": "cds", "sequence": "contig_1", "start": 29912, "stop": 30244, "strand": "+", "frame": 2, "gene": "rpoE", "product": "DNA-directed RNA polymerase subunit delta", "db_xrefs": [ "COG:COG3343", "COG:K", "EC:2.7.7.6", "GO:0000428", "GO:0003899", "GO:0006351", "GO:0006355", "KEGG:K03048", "SO:0001217", "UniRef:UniRef50_P47268", "UniRef:UniRef90_P75090" ], "nt": "GTGAAGCACTTTAAGATCTCCAAACAAGACGAACCTAACCTAATTTCGCGGTTTTACCAAGACTTTTTGGAAGACCCTAACTTTGTTTATTTAGGGGAACGCAACTGGAAGTTAAGGGACTTCATGAAGTTTGATGAATGGAACAAGATTTCCCAAGCAATGTTTGTGACCAAGGAAATCTTTGAAGAGGGTTATGAAGACCTGTCCAACAAGAAGAAGGAAAACGAGGAAGAAGTGAATGACTTCATTATGGGCAATGATGGTGATGACAACAGTACCGGTGATGAAATTGTGCAAGGACTCATTAACGATTTTCGCGACGATAACCAATAA", "aa": "MKHFKISKQDEPNLISRFYQDFLEDPNFVYLGERNWKLRDFMKFDEWNKISQAMFVTKEIFEEGYEDLSNKKKENEEEVNDFIMGNDGDDNSTGDEIVQGLINDFRDDNQ", "aa_hexdigest": "42aaa3b7158ae8156e8956ee3696c374", "start_type": "GTG", "rbs_motif": null, "genes": [ "rpoE" ], "truncated": "5-prime", "pseudogene": { "start": 29804, "stop": 30244, "observations": { "mutation": [ 29900 ], "stop": [ 29900 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75090", "query_cov": 1.0, "subject_cov": 0.7534246575342466, "identity": 0.982, "score": 222.0, "evalue": 5.34e-73, "gene_start": 1, "gene_end": 110, "reference_start": 37, "reference_end": 146, "reference_sequence": "MTMQVEYLDLISQAKEIAETQFKAEPFSFDAIWKEVVKHFKISKQDEPNLISRFYQDFLEDPNFVYLGERNWKLRDFMKFDEWNKISQAMFVTKEIFEEGYEDLSNKKKENEEEVNDFIMGNDGDDNSTGDEIVQGLINDFRDDKQ" }, "paralog": false, "identity": 0.9863013698630136, "score": 294.0, "evalue": 7.22e-104, "description": "Internal stop codon at 29900. Nonsense mutation around 29900.", "psc": { "uniref90_id": "UniRef90_P75090", "gene": "rpoE", "product": "DNA-directed RNA polymerase subunit delta", "ec_ids": [ "2.7.7.6" ], "uniref50_id": "UniRef50_P47268", "cog_id": "COG3343", "cog_category": "K", "kegg_orthology_id": "K03048", "go_ids": [ "GO:0000428", "GO:0003899", "GO:0006351", "GO:0006355" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75090", "EC:2.7.7.6", "UniRef:UniRef50_P47268", "COG:COG3343", "COG:K", "KEGG:K03048", "GO:0000428", "GO:0003899", "GO:0006351", "GO:0006355" ] }, "pscc": { "uniref50_id": "UniRef50_P47268", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47268" ], "product": "Probable DNA-directed RNA polymerase subunit delta" } }, "id": "APKECPOBEN_91", "locus": "GCA002090215_00050" }, { "type": "cds", "sequence": "contig_1", "start": 30244, "stop": 30795, "strand": "+", "frame": 1, "gene": null, "product": "Fructose-bisphosphate aldolase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P7V3" ], "nt": "ATGCTAGTAAACATCAAACAAATGTTGCAACACGCTAAGCAACACCACTATGCTGTACCCCACATTAATATCAATAACTATGAATGGGCTAAGGCAGTATTAACAGCGGCTCAGCAGGCCAAGAGTCCGATTATTGTCTCCACTTCTGAGGGCGCTTTAAAGTATATTTCCGGACACCAAGTGGTAGTACCGATGGTAAAGGGCTTAGTCGATGCTTTAAAGATTACTGTACCCGTAGCACTCCACTTAGACCATGGTAGTTATGAAGGTTGTAAGGCAGCGTTGCAAGCTGGGTTTAGTTCCATTATGTTTGATGGCTCGCATTTACCCTTTCAAGAAAACTTCACTAAATCCAAGGAGTTAATTGAACTAGCTAAACAAACCAATGCTTCTGTGGAACTAGAAGTGGGTACCTTAGGTGGGGAAGAAGATGGTATTGTGGGTCAAGGTGAATTGGCCAACATTGAAGAGTGCAAGCAAATTGCGACCTTAAAACCGGATGCCTTAGCTGCTGGGATTGGTAACATCCACGGGCTTTACCCCGACAACTGA", "aa": "MLVNIKQMLQHAKQHHYAVPHININNYEWAKAVLTAAQQAKSPIIVSTSEGALKYISGHQVVVPMVKGLVDALKITVPVALHLDHGSYEGCKAALQAGFSSIMFDGSHLPFQENFTKSKELIELAKQTNASVELEVGTLGGEEDGIVGQGELANIEECKQIATLKPDALAAGIGNIHGLYPDN", "aa_hexdigest": "78d2d608f49745d44e6fd1c34820c695", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002FEF401E", "query_cov": 0.9016393442622951, "subject_cov": 0.9375, "identity": 0.63, "score": 211.0, "evalue": 6.84e-67, "valid": false, "product": "class II fructose-bisphosphate aldolase", "ec_ids": [ "4.1.2.13" ], "uniref50_id": "UniRef50_A0A3B0P7V3", "kegg_orthology_id": "K01624" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0P7V3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P7V3" ], "product": "Fructose-bisphosphate aldolase" }, "genes": [], "id": "APKECPOBEN_92", "locus": "GCA002090215_00051" }, { "type": "cds", "sequence": "contig_1", "start": 30853, "stop": 31110, "strand": "+", "frame": 1, "gene": null, "product": "fructose-bisphosphate aldolase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A097ST37" ], "nt": "GTGCTCCATGGTGGTTCGGGAATTCCGGAAGCGGATGTCAAAAAAGCAATTGGCTTAGGCATTAGCAAGCTCAACATTAACACTGAATGTCAACTAGCGTTTGCTAAAGCGATTCGGGAATATGTGGAAGCCAAGAAGGACTTAGATACCCACAATAAGGGCTATGACCCACGGAAATTGTTAAAGTCACCTACCCAAGCAATTGTGGACTGCTGCTTGGAAAAGATGCAGCTCTGTGGTTCAACCAATAAGGCTTAG", "aa": "MLHGGSGIPEADVKKAIGLGISKLNINTECQLAFAKAIREYVEAKKDLDTHNKGYDPRKLLKSPTQAIVDCCLEKMQLCGSTNKA", "aa_hexdigest": "75143f24b9163c163db3272e7b8045dc", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A097ST37", "query_cov": 1.0, "subject_cov": 0.8585858585858586, "identity": 0.647, "score": 119.0, "evalue": 2.17e-33, "valid": false, "product": "fructose-bisphosphate aldolase", "ec_ids": [ "4.1.2.13" ], "uniref50_id": "UniRef50_A0A097ST37", "kegg_orthology_id": "K01624" }, "pscc": { "uniref50_id": "UniRef50_A0A097ST37", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A097ST37" ], "product": "fructose-bisphosphate aldolase" }, "genes": [], "id": "APKECPOBEN_93", "locus": "GCA002090215_00052" }, { "type": "cds", "sequence": "contig_1", "start": 31111, "stop": 32091, "strand": "+", "frame": 1, "gene": "ychF", "product": "redox-regulated ATPase YchF", "db_xrefs": [ "GO:0005524", "GO:0005525", "GO:0005737", "GO:0016887", "GO:0043023", "GO:0046872", "SO:0001217", "UniRef:UniRef50_P37518", "UniRef:UniRef90_P75088" ], "nt": "ATGTTAAGTGCGGGAATTGTTGGTTTACCCAACGTTGGTAAATCAACCTTGTTTAGTGCTATTACCAACCTGCAAGTGGAAATAGCTAACTATCCCTTTGCCACGATTGAACCGAATGCCGGGATAGTTAACGTAATCGATGAGCGCTTAGACAAACTAGCGAGTTTAATTAAACCCGATAAGGTTACCCACACTACCTTTCGCTTTGTTGACATTGCTGGTTTAGTTAAGGGTGCCAGTAAGGGAGAAGGACTGGGCAACCAGTTCTTGGCCAACATCAGAGAAGTTGACCTTATTTGTCACGTAGTGCGTTGCTATGAGGACAAGAAGATTGTCCACGTTAATAACCAGGTCGATCCCGTATTTGACTTTGAAATTATTGTCAATGAGTTAATCCAGGCCGACATTGAGGTGGTGAATACCCGGATTGGCAAGATTAAGCGCAAGGCCGAATCTGGTGATAAGCAGTCCAAGGAAGAATATCAACTGCTAGCACCCGTTTTACAAGGTTTGCAACAAAACCAGATGGTGTTGCATCTAGTGAATGAAGTAGATTTAAAGAAACTCAAGTCACTTAATTTACTAACCGCCAAACCGATCCTAGTGGTGGCCAATGTGTCAGAAGCGGATTTGTCCAACCTTGACCATAATCCCCACCTCACTCAGCTCAACCAGTTCTTAAAACAACATAACTTACCCCATGCCATTCCTGTTTGTGCATTATTAGAAAATGAGTTGAGTAGCTTGGATGCCAATGGTCGTCAAGACTGGCTCAAGGAGTTGGGTTTAAGTGATTACCAAGGTTTAAACCAACTAATTAAAACGGCTTATGATGCGATTGGTCTGTGGTCATTCTTTACCTTTGGTAAGCAAGAAGTGCGCGCTTGGGCTTTTAAAAAGGGTTGGTTAGCACCGCAGTGTGCGGGTGAAATTCACACTGACTTTGAACGTGGTTTTATTAAAGTAGAAGTAATTAGTTGA", "aa": "MLSAGIVGLPNVGKSTLFSAITNLQVEIANYPFATIEPNAGIVNVIDERLDKLASLIKPDKVTHTTFRFVDIAGLVKGASKGEGLGNQFLANIREVDLICHVVRCYEDKKIVHVNNQVDPVFDFEIIVNELIQADIEVVNTRIGKIKRKAESGDKQSKEEYQLLAPVLQGLQQNQMVLHLVNEVDLKKLKSLNLLTAKPILVVANVSEADLSNLDHNPHLTQLNQFLKQHNLPHAIPVCALLENELSSLDANGRQDWLKELGLSDYQGLNQLIKTAYDAIGLWSFFTFGKQEVRAWAFKKGWLAPQCAGEIHTDFERGFIKVEVIS", "aa_hexdigest": "3c04210cf453a8bcfb7d85eba911b15b", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75088", "query_cov": 1.0, "subject_cov": 0.8907103825136612, "identity": 1.0, "score": 637.0, "evalue": 6.5e-230, "valid": true, "gene": "ychF", "product": "redox-regulated ATPase YchF", "uniref50_id": "UniRef50_P37518", "go_ids": [ "GO:0005524", "GO:0005525", "GO:0005737", "GO:0016887", "GO:0043023", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_P37518", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P37518" ], "product": "Ribosome-binding ATPase YchF" }, "genes": [ "ychF" ], "id": "APKECPOBEN_94", "locus": "GCA002090215_00053" }, { "type": "cds", "sequence": "contig_1", "start": 32336, "stop": 32425, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAAAACCAACTAAGAACCTCACCGTTAACCTAATTAACATTGGTTTTGATGCCTATGCCGACAGTCAAAAAGCGCTGTTAGCTTGA", "aa": "MLKPTKNLTVNLINIGFDAYADSQKALLA", "aa_hexdigest": "cf3b05b5fcb41f59a991ef033f1ad52e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3163.684399999999, "isoelectric_point": 8.18930606842041 }, "id": "APKECPOBEN_95", "locus": "GCA002090215_00054" }, { "type": "cds", "sequence": "contig_1", "start": 32615, "stop": 33025, "strand": "-", "frame": 3, "gene": "rimL", "product": "putative protein MPN_027", "db_xrefs": [ "COG:COG1670", "COG:JO", "GO:0016747", "SO:0001217", "UniRef:UniRef50_P75087", "UniRef:UniRef90_P75087" ], "nt": "ATGGATAAAAACACTATTCTTGAGTTTTGCTTTTTTGGTGATCGTTACCAACTAATGGCACTGAAAAACCCCAATAAGTTGGTCAAAAATAAACAGGTCAAAGCTGCTTTTAATGTTTTCATGAAGCACCGGACCACCTTAAGTGCTACTAACTATATGGCGCAAATTGTGGAAAACTTTGAGCAGTTCTTGGAATACATCCACGACAAACTGCAACCCGGTAAACTGTTAGCGCTCTTATTTAAAAACAAGCAGTTTTTGGGTTGGTTTTGTGTCGGGCAATGGGGCAACATGCCGGTATTATCGTTTTGGATTGATCCCAGTCAACAACAAAGTGGTGTCGCTTTAGAAACAATTAGTTTCTTTCTTAAGTACTACCAGCGTACGTTGCAACCGCGGGAATTAGTGTGA", "aa": "MDKNTILEFCFFGDRYQLMALKNPNKLVKNKQVKAAFNVFMKHRTTLSATNYMAQIVENFEQFLEYIHDKLQPGKLLALLFKNKQFLGWFCVGQWGNMPVLSFWIDPSQQQSGVALETISFFLKYYQRTLQPRELV", "aa_hexdigest": "b1a406132be46032d970c6555964dee1", "start_type": "ATG", "rbs_motif": null, "genes": [ "rimL" ], "truncated": "5-prime", "pseudogene": { "start": 32312, "stop": 33025, "observations": { "mutation": [ 32338, 32440, 32452, 32617 ], "stop": [ 32338, 32440, 32452, 32617 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75087", "query_cov": 1.0, "subject_cov": 0.49635036496350365, "identity": 1.0, "score": 283.0, "evalue": 9.72e-95, "gene_start": 1, "gene_end": 136, "reference_start": 1, "reference_end": 136, "reference_sequence": "MDKNTILEFCFFGDRYQLMALKNPNKLVKNKQVKAAFNVFMKHRTTLSATNYMAQIVENFEQFLEYIHDKLQPGKLLALLFKNKQFLGWFCVGQWGNMPVLSFWIDPSQQQSGVALETISFFLKYYQRTLQPRELVWSFYESNQRSRHLANKLGFPSLFYFKKAEPNAPKVYFGTKDFKGESVIETKPYLGWGSGWKVNQMLKPTKNLTVNLINIGFDAYADSQKALLAWFKTHKLTKGQVEVFFNYELCCLCISYNHKQSWVVYSFQQLLAKR" }, "paralog": false, "identity": 0.9831223628691983, "score": 471.0, "evalue": 3.81e-171, "description": "Internal stop codon at 32338, 32440, 32452, 32617. Nonsense mutation around 32338, 32440, 32452, 32617.", "psc": { "uniref90_id": "UniRef90_P75087", "gene": "RimL", "product": "Uncharacterized protein MPN_027", "uniref50_id": "UniRef50_P75087", "cog_id": "COG1670", "cog_category": "JO", "go_ids": [ "GO:0016747" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75087", "UniRef:UniRef50_P75087", "COG:COG1670", "COG:JO", "GO:0016747" ] }, "pscc": { "uniref50_id": "UniRef50_P75087", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75087" ], "product": "Uncharacterized protein MPN_027" } }, "id": "APKECPOBEN_96", "locus": "GCA002090215_00055" }, { "type": "cds", "sequence": "contig_1", "start": 33058, "stop": 33279, "strand": "+", "frame": 1, "gene": null, "product": "glycosyltransferase family 2 protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001ED93A4E" ], "nt": "GTGCAATTCAAGTATTTATTCACCGTGATTATTCCCACTTACAATTGTGGTCAATACATTCCAAAAGCATTAGATTCCTTGCTTTTACAAGGTGAATATTTTACTAAGACCCAAGTTTTAATCGTCAATGATGGTTCGACTGATAATACCAAGCAGATCGTAGAACCCTATACCCAACAATACAGTAACATTGAGTACTTGGAAAAACCCAATGGCAATTGA", "aa": "MQFKYLFTVIIPTYNCGQYIPKALDSLLLQGEYFTKTQVLIVNDGSTDNTKQIVEPYTQQYSNIEYLEKPNGN", "aa_hexdigest": "e4c2ed2ab68fc1a238982e4aaf22c83d", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001ED93A4E", "query_cov": 1.0, "subject_cov": 0.8588235294117647, "identity": 0.767, "score": 113.0, "evalue": 1.71e-31, "valid": false, "product": "glycosyltransferase family 2 protein", "uniref50_id": "UniRef50_UPI001ED93A4E" }, "pscc": { "uniref50_id": "UniRef50_UPI001ED93A4E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001ED93A4E" ], "product": "glycosyltransferase family 2 protein" }, "genes": [], "id": "APKECPOBEN_97", "locus": "GCA002090215_00056" }, { "type": "cds", "sequence": "contig_1", "start": 33286, "stop": 33711, "strand": "+", "frame": 1, "gene": "wcaA", "product": "putative glycosyltransferase MG025-like protein", "db_xrefs": [ "COG:COG0463", "COG:M", "EC:2.4.-.-", "GO:0016757", "SO:0001217", "UniRef:UniRef50_P75086", "UniRef:UniRef90_P75086" ], "nt": "GTGGTCAACTTTGTCAAACAAAACCAGTTAGCTAAGGGTCAGTACATTACTGTATTAGACAGCGATGATTATTTTCTAGCTAACGCTTTTCAACGCGTAGCTGCTCACTTTGGCCATGACATGATTGTGAGCGCCTTTTACTGTTACATTAGCCCTAAAAGAAGGCGTTTTCTCAAACCATACTTTGGTAAAACGGGGGTAATTGAACAAAAGACTAAACTACGCACACCCCATTCCCAACCCTTGGCTAAGTTCTACCGCCACGAGATCTTTCACTTGCTAGATCCTTTAAAAGAAAAGCTGTTTTACCAGGACTGTCTGTTGTACCATAATGCCATCAATAAGGTGCAAAGTGTCTTTTACATTTGTGAACCCTTAGCAGTGTGGTATGCCACTAGACCAGGTAATTCCACCACTATGCCCTGA", "aa": "MVNFVKQNQLAKGQYITVLDSDDYFLANAFQRVAAHFGHDMIVSAFYCYISPKRRRFLKPYFGKTGVIEQKTKLRTPHSQPLAKFYRHEIFHLLDPLKEKLFYQDCLLYHNAINKVQSVFYICEPLAVWYATRPGNSTTMP", "aa_hexdigest": "322a34765c54fb633cbe6abc50bf06c6", "start_type": "GTG", "rbs_motif": null, "genes": [ "wcaA" ], "truncated": "both", "pseudogene": { "start": 33058, "stop": 33957, "observations": { "mutation": [ 33277, 33709, 33874 ], "stop": [ 33277, 33709, 33874 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75086", "query_cov": 1.0, "subject_cov": 0.47157190635451507, "identity": 0.993, "score": 294.0, "evalue": 1.06e-98, "gene_start": 1, "gene_end": 141, "reference_start": 77, "reference_end": 217, "reference_sequence": "MQFKYLFTVIIPTYNCGQYIPKALDSLLLQGEYFTKTQVLIVNDGSTDNTKQIVEPYTQQYSNIEYLEKPNGNWGSVVNFVKQNQLAKGQYITVLDSDDYFLANAFQRVAAHFGHDMIVSAFYCYISPKRRRFLKPYFGKTGVIEQKTKLRTPHSQPLAKFYRHEIFHLLDPLKEKLFYQDCLLYHNAINKVQSVFYICEPLAVWYATRPGNSTTMPWNNADKFQAWCDLLKQMNLYGAGIVIYIYTMLPGFLKELKRQQLVLDLAKKPAYTWLPQPLAFLFGGLMALRTRKYIRYPKN" }, "paralog": false, "identity": 0.9866220735785953, "score": 605.0, "evalue": 7.97e-224, "description": "Internal stop codon at 33277, 33709, 33874. Nonsense mutation around 33277, 33709, 33874.", "psc": { "uniref90_id": "UniRef90_P75086", "gene": "WcaA", "product": "Uncharacterized glycosyltransferase MG025 homolog", "ec_ids": [ "2.4.-.-" ], "uniref50_id": "UniRef50_P75086", "cog_id": "COG0463", "cog_category": "M", "go_ids": [ "GO:0016757" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75086", "EC:2.4.-.-", "UniRef:UniRef50_P75086", "COG:COG0463", "COG:M", "GO:0016757" ] }, "pscc": { "uniref50_id": "UniRef50_P75086", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75086" ], "product": "Uncharacterized glycosyltransferase MG025 homolog" } }, "id": "APKECPOBEN_98", "locus": "GCA002090215_00057" }, { "type": "cds", "sequence": "contig_1", "start": 33757, "stop": 33876, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACTTATACGGCGCGGGTATAGTGATTTACATTTACACTATGTTACCTGGTTTTTTAAAAGAATTAAAACGGCAACAACTAGTGTTAGATTTAGCTAAAAAACCCGCTTATACCTGA", "aa": "MNLYGAGIVIYIYTMLPGFLKELKRQQLVLDLAKKPAYT", "aa_hexdigest": "3898c01e7da5abe6be54c273b7f2bc05", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4474.375000000002, "isoelectric_point": 9.522770118713382 }, "id": "APKECPOBEN_99", "locus": "GCA002090215_00058" }, { "type": "cds", "sequence": "contig_1", "start": 33978, "stop": 34550, "strand": "+", "frame": 3, "gene": "efp", "product": "elongation factor P", "db_xrefs": [ "COG:COG0231", "COG:J", "GO:0003746", "GO:0005737", "GO:0006414", "GO:0043043", "KEGG:K02356", "RefSeq:WP_010874386.1", "SO:0001217", "UniParc:UPI0000129D0D", "UniRef:UniRef100_P75085", "UniRef:UniRef50_P75085", "UniRef:UniRef90_P75085" ], "nt": "ATGGCAGACATGATTGAAGCGAAAAGTTTACGCAGTGGACAAACGATTTTTGGTCCCAATAAAGAGATTCTGTTGGTGTTAGAAAACACCTTTAATAAGACCGCAATGCGCCAGGGAATTGTTAAAACCAAGGTCAAAAACTTACGCACTGGGGCCATTGTTTGGATTGAGTTTACCGGAGACAAGTTAGAACAAGTCATTATTGACAAGAAGAAAATGACCTTCTTGTACAAGGATGGTGCTAACTATGTTTTTATGGACCAACAAGACTACAGTCAAATTGAGATCCCCGAAAAGCAGTTAGAGTGGGAAAAGAACTTTATCACTGAAGACAGTGAAGTTACTATTATTAGCTACCAAAGTGAAATTTTGGGCGTTAACCTACCCGAATTAGTGCCGATTGAAGTGGAATTTGCCGAAGAAGCAGTGCAAGGTAATACCGCTAACATGGCGCGTAAACGGGCTCGGTTAGTTTCGGGTTACGAATTAGATGTGCCCCAGTTCATTCGTACTGGTGATAAGATTGTGATTTCCACCATTGACGGGAGCTATCGTGAACGGTACAACAAGTAA", "aa": "MADMIEAKSLRSGQTIFGPNKEILLVLENTFNKTAMRQGIVKTKVKNLRTGAIVWIEFTGDKLEQVIIDKKKMTFLYKDGANYVFMDQQDYSQIEIPEKQLEWEKNFITEDSEVTIISYQSEILGVNLPELVPIEVEFAEEAVQGNTANMARKRARLVSGYELDVPQFIRTGDKIVISTIDGSYRERYNK", "aa_hexdigest": "ed93e08bd627e0e94ccd9d96fdcbd1c0", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000129D0D", "ncbi_nrp_id": "WP_010874386.1", "uniref100_id": "UniRef100_P75085", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000129D0D", "RefSeq:WP_010874386.1", "UniRef:UniRef100_P75085" ] }, "ips": { "uniref100_id": "UniRef100_P75085", "uniref90_id": "UniRef90_P75085" }, "psc": { "uniref90_id": "UniRef90_P75085", "gene": "efp", "product": "elongation factor P", "uniref50_id": "UniRef50_P75085", "cog_id": "COG0231", "cog_category": "J", "kegg_orthology_id": "K02356", "go_ids": [ "GO:0003746", "GO:0005737", "GO:0006414", "GO:0043043" ] }, "pscc": { "uniref50_id": "UniRef50_P75085", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75085" ], "product": "Elongation factor P" }, "genes": [ "efp" ], "id": "APKECPOBEN_100", "locus": "GCA002090215_00059" }, { "type": "cds", "sequence": "contig_1", "start": 34531, "stop": 34974, "strand": "+", "frame": 1, "gene": "nusB", "product": "putative MG027-like protein", "db_xrefs": [ "COG:COG0781", "COG:K", "RefSeq:WP_014574845.1", "SO:0001217", "UniParc:UPI0001DD6462", "UniRef:UniRef100_A0AAN4XFP4", "UniRef:UniRef50_P47273", "UniRef:UniRef90_P75084" ], "nt": "GTGAACGGTACAACAAGTAAATTAACTAGAACCCAAAGACGGATTGCAATTGTGGAGTTTATCTTCGCCACGCTGTTCTTTTTACCGAAAACTGCTGACCAAATCCAAGCGGCCTTTTTGGACTATGATGTGCCAGAACGTCCGCTCAACGATTGGCAAAAAGAGATTGTCAAAGTCTTTAGTGAACGTTGTGTGGAATTCATTGAACTAATTGAAAACCAACAACAACGCAACCAAGCAGAAGTACAAAGCAAGTACAACAAGGTTTCCGGTAAAAAAGTCGACTTATTGACTAAGGCCGTGATTCTCTGTGCTTTGAGCGAACAACACGCGCAAGCGACTGATAAACCATTATTAATTAGTGAAGCCTTACTGATTATGGACCACTATTCCCAGGTACCCGAAAAGAAGCAAACCCATGCTTTATTGGATAAACTCTTGTAA", "aa": "MNGTTSKLTRTQRRIAIVEFIFATLFFLPKTADQIQAAFLDYDVPERPLNDWQKEIVKVFSERCVEFIELIENQQQRNQAEVQSKYNKVSGKKVDLLTKAVILCALSEQHAQATDKPLLISEALLIMDHYSQVPEKKQTHALLDKLL", "aa_hexdigest": "e029943092bf8c0c09f57c14baf6f38b", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD6462", "ncbi_nrp_id": "WP_014574845.1", "uniref100_id": "UniRef100_A0AAN4XFP4", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD6462", "RefSeq:WP_014574845.1", "UniRef:UniRef100_A0AAN4XFP4" ] }, "ips": { "uniref100_id": "UniRef100_A0AAN4XFP4", "uniref90_id": "UniRef90_P75084" }, "psc": { "uniref90_id": "UniRef90_P75084", "gene": "NusB", "product": "Uncharacterized protein MG027 homolog", "uniref50_id": "UniRef50_P47273", "cog_id": "COG0781", "cog_category": "K" }, "pscc": { "uniref50_id": "UniRef50_P47273", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47273" ], "product": "Uncharacterized protein MG027" }, "genes": [ "nusB" ], "id": "APKECPOBEN_101", "locus": "GCA002090215_00060" }, { "type": "cds", "sequence": "contig_1", "start": 35004, "stop": 35213, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTTGTAGCAAACCTAGCCTTAGTGTTGGGTTTTATGTTGAACATTGTGGTGGCGCGGTATACCTTAACTGGTGCTACGCCCCAAGCACGTTTTTTATTCCTCACACCCTTTTTAGGCATAGTAGCAGCGAGTATCTTCTACTTCTTTGATGTGAAGTGGTTCTTAGCGGATTATCCCTACAAGAAGTTCCATTTTCAAAAGAAGTGA", "aa": "MFVANLALVLGFMLNIVVARYTLTGATPQARFLFLTPFLGIVAASIFYFFDVKWFLADYPYKKFHFQKK", "aa_hexdigest": "7c4ba972575b761923c09e97ff050c0d", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8018.525199999996, "isoelectric_point": 9.90029697418213 }, "id": "APKECPOBEN_102", "locus": "GCA002090215_00061" }, { "type": "cds", "sequence": "contig_1", "start": 35370, "stop": 35585, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGGTGTTAGTATCTTTTTGAGCTTAATTAGTATTGGTTTGAGCAAAACAGCCCACTTTAAAATTGATGTGGAAATGCTCAAAGCGAAAAAAGGCGAACCCACCGCCGCTGATAAAACCGACAGCCGGCCGGTAGTTGTTGATTTAGACCAAACTAAATCAAAAAAAGATGGGGATAACCCACCCCAAGCTTCGGGTGATATGACTAGTCTTTAG", "aa": "MGVSIFLSLISIGLSKTAHFKIDVEMLKAKKGEPTAADKTDSRPVVVDLDQTKSKKDGDNPPQASGDMTSL", "aa_hexdigest": "b66b2a8944333d45e3b66390bb9894b4", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7560.590500000006, "isoelectric_point": 6.538212013244629 }, "id": "APKECPOBEN_103", "locus": "GCA002090215_00062" }, { "type": "cds", "sequence": "contig_1", "start": 35809, "stop": 36135, "strand": "-", "frame": 1, "gene": "yajL", "product": "putative MG029-like protein", "db_xrefs": [ "COG:COG0693", "COG:V", "GO:0005737", "RefSeq:WP_010874389.1", "SO:0001217", "UniParc:UPI0000139135", "UniRef:UniRef100_P75082", "UniRef:UniRef50_P75082", "UniRef:UniRef90_P75082" ], "nt": "ATGAAAAAACTCTTAGTCATTGTTTACCCTGACATGAATGATGTGGAATACACCAACACAATGGTGGTATTTGGTTTTGTTAAAGAATTACAAACCGTGATTTATCACCCTAACTTAAGTACAGTTAAGGGCAGTAATGGGGTAACTTTAGTAAACCAGATTACTAGCAAGGTTAATCTGGAGGAATTTGATGGTGTCTTTATCCCTGGCGGTATGGGAGCTACCAAGGTATTAGACCATGACCAACAGTTACTCGACACTATCCGTTACTTTAAGGATCATGACAAGTATGTCTTTGCCATTTGTGATACCCCCAACGTGTTGTAA", "aa": "MKKLLVIVYPDMNDVEYTNTMVVFGFVKELQTVIYHPNLSTVKGSNGVTLVNQITSKVNLEEFDGVFIPGGMGATKVLDHDQQLLDTIRYFKDHDKYVFAICDTPNVL", "aa_hexdigest": "df17031a769821fbbb19ec1969c9a74d", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000139135", "ncbi_nrp_id": "WP_010874389.1", "uniref100_id": "UniRef100_P75082", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139135", "RefSeq:WP_010874389.1", "UniRef:UniRef100_P75082" ] }, "ips": { "uniref100_id": "UniRef100_P75082", "uniref90_id": "UniRef90_P75082" }, "psc": { "uniref90_id": "UniRef90_P75082", "gene": "YajL", "product": "Uncharacterized protein MG029 homolog", "uniref50_id": "UniRef50_P75082", "cog_id": "COG0693", "cog_category": "V", "go_ids": [ "GO:0005737" ] }, "pscc": { "uniref50_id": "UniRef50_P75082", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75082" ], "product": "Uncharacterized protein MG029 homolog" }, "genes": [ "yajL" ], "id": "APKECPOBEN_104", "locus": "GCA002090215_00063" }, { "type": "cds", "sequence": "contig_1", "start": 36139, "stop": 36759, "strand": "-", "frame": 1, "gene": "upp", "product": "uracil phosphoribosyltransferase", "db_xrefs": [ "COG:COG0035", "COG:F", "EC:2.4.2.9", "GO:0000287", "GO:0004845", "GO:0005525", "GO:0006223", "GO:0044206", "RefSeq:WP_010874390.1", "SO:0001217", "UniParc:UPI0000137D2C", "UniRef:UniRef100_P75081", "UniRef:UniRef50_P47276", "UniRef:UniRef90_P75081" ], "nt": "GTGATCAAAAAAGTGCACCACGCTTTGATCGAAAACGAATTGACAAAGCTACGTGATAAATCTTCTTCCTCCTCGCAATTTCGGATGGCGTTAAGCCAAATCACTTCACTACTCTTTTTTGAAGTAACTAAGGAATCACCCTTAGAAGAAATTGTTGTAAAAACCCCGTTTACCAAAACGAAGGGTTATAAGCTCAAAAACGAAATTGTTCTAGTCCCCATAATGCGCGCTGGCCTGGGTATGGTTGACCCAATTGTGCGCTATTCGGAAAAGATTCGTGTGGGTCACTTAGGGATCTACCGTGAACATGAGGGCACTAATGTAATTTCTTACTATAAGAAAATGCCCGAAAACATTGGCAACTCCCACGTGATTATCTTAGACCCAATGCTAGCTACTGGTACTACCCTAGCCACAGCCATTAGTTCCATTAAAAAGAACCACCCGATTAAGATTAGTGTGGTGGCCATAGTAGCAGCACCAGAGGGCATTAAAGCCGTGGAAGCAGCGCACCCTGATGTGGACATTTACCTAGCAGCCATTGATGAAAAATTAAATAAGGATAATTACATTATTCCCGGGCTTGGTGATGCTGGGGATCGCTTATTTGGTACTAAATAA", "aa": "MIKKVHHALIENELTKLRDKSSSSSQFRMALSQITSLLFFEVTKESPLEEIVVKTPFTKTKGYKLKNEIVLVPIMRAGLGMVDPIVRYSEKIRVGHLGIYREHEGTNVISYYKKMPENIGNSHVIILDPMLATGTTLATAISSIKKNHPIKISVVAIVAAPEGIKAVEAAHPDVDIYLAAIDEKLNKDNYIIPGLGDAGDRLFGTK", "aa_hexdigest": "92a11133979695b8a841da5a0124591c", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000137D2C", "ncbi_nrp_id": "WP_010874390.1", "uniref100_id": "UniRef100_P75081", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000137D2C", "RefSeq:WP_010874390.1", "UniRef:UniRef100_P75081" ] }, "ips": { "uniref100_id": "UniRef100_P75081", "uniref90_id": "UniRef90_P75081" }, "psc": { "uniref90_id": "UniRef90_P75081", "gene": "upp", "product": "uracil phosphoribosyltransferase", "ec_ids": [ "2.4.2.9" ], "uniref50_id": "UniRef50_P47276", "cog_id": "COG0035", "cog_category": "F", "go_ids": [ "GO:0000287", "GO:0004845", "GO:0005525", "GO:0006223", "GO:0044206" ] }, "pscc": { "uniref50_id": "UniRef50_P47276", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47276" ], "product": "Uracil phosphoribosyltransferase" }, "genes": [ "upp" ], "id": "APKECPOBEN_105", "locus": "GCA002090215_00064" }, { "type": "cds", "sequence": "contig_1", "start": 36799, "stop": 37260, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF12836.13", "PFAM:PF14579.12" ], "nt": "TTGAGCTACTATGCTTGTTTGTTGAGCTTTAAACTCAAGGAACATGATGTCAGTGGTTTTAAATCAGGTGTGAGCTTTGTCAAGCAAAAACTAGAGGAGCTCAACACACTGTACCGCATTAAACGGATTAAACCCAAAGAAGCAGAACTGTTAACCAGTTACGAGGTTTATTTAGAGATGATGGCCCGCGGCATTAAACTAGAACAAATCTCTTTAACCCACTCCCACGCCACTCGTTTTGTCGAGCACAACGGCATGCTAATTGCCCCTTTTATTACGATTCCGGGGATGGGGGAAGCTGTGGCCAATTCGATCATAGAAGCGCGTAACGAAAAGCCCTTTAGTTCGCTCGATGACTTTAAAAAACGCACTAAAATTACCAAAAAACACATTGAAGCGTTTACCCAAATGCAACTACTCGATGAGTTTAGGGAGCAAGACAACCAGAAAAAACTTTTTTAG", "aa": "MSYYACLLSFKLKEHDVSGFKSGVSFVKQKLEELNTLYRIKRIKPKEAELLTSYEVYLEMMARGIKLEQISLTHSHATRFVEHNGMLIAPFITIPGMGEAVANSIIEARNEKPFSSLDDFKKRTKITKKHIEAFTQMQLLDEFREQDNQKKLF", "aa_hexdigest": "e85c6b3db54daca18ee297e049f7f416", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF12836.13", "name": "HHH_3", "length": 42, "aa_cov": 0.2679738562091503, "hmm_cov": 1.0, "evalue": 3.0594709944915465e-09, "score": 32.35352325439453, "start": 93, "stop": 133 }, { "id": "PF14579.12", "name": "HHH_6", "length": 87, "aa_cov": 0.5424836601307189, "hmm_cov": 0.9885057471264368, "evalue": 6.377497536958984e-16, "score": 53.87648010253906, "start": 64, "stop": 146 } ], "seq_stats": { "molecular_weight": 17792.491299999987, "isoelectric_point": 9.097536659240724 }, "id": "APKECPOBEN_106", "locus": "GCA002090215_00065" }, { "type": "cds", "sequence": "contig_1", "start": 37291, "stop": 37824, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF17657.6" ], "nt": "ATGTTAAAGCACCTGGCCGATCTTACCCATGTCAATCCACAAAACATTCCCCGGTTTGACAAGAAGCTCACCGAAATGTTTTGGTCGGTCAATCCCTTAAAACTAAAACCGCACTACTTAGATGAACCCACGGGAGCGATTGGGATTCCCGAGTTTGGTACTAAGTTCGTTCGCAAGATCTTGGAACAAACCAAACCAAAGGGCTTTGGTGACCTCATTCGCGTCTCGGGTCTAAGTCACGGGAAGAACGTGTGGGCGGACAATGCGCAGAAAATCTTGAAGGACCAAAACTTAAGCTTAAAGGATGTAATTGCCTGTCGCGATGACATTATGCTCTACCTCATTCATAAGGGGATGCAGGCCAAAGATGCTTTTGAGATCATGGAAAAGGTGCGTAAGGGAATTGCTCTCAATGCCAAGGAAGTGCAGTTAATGCAGAGCAATGGGGTCGAACAACACTGGATTAATTCGTGTTTAAAGATTAGCTACCTGTTCCCGAAAGCCCACGCCGCGGCTTATGTTTTAATGGCCTGA", "aa": "MLKHLADLTHVNPQNIPRFDKKLTEMFWSVNPLKLKPHYLDEPTGAIGIPEFGTKFVRKILEQTKPKGFGDLIRVSGLSHGKNVWADNAQKILKDQNLSLKDVIACRDDIMLYLIHKGMQAKDAFEIMEKVRKGIALNAKEVQLMQSNGVEQHWINSCLKISYLFPKAHAAAYVLMA", "aa_hexdigest": "8fa18bb424c0300ae655adab1ba74e3c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF17657.6", "name": "DNA_pol3_finger", "length": 162, "aa_cov": 0.7853107344632768, "hmm_cov": 0.9135802469135802, "evalue": 1.1595709503791235e-25, "score": 85.28016662597656, "start": 2, "stop": 140 } ], "seq_stats": { "molecular_weight": 20107.401399999995, "isoelectric_point": 9.373783683776853 }, "id": "APKECPOBEN_107", "locus": "GCA002090215_00066" }, { "type": "cds", "sequence": "contig_1", "start": 37903, "stop": 38829, "strand": "-", "frame": 1, "gene": null, "product": "DNA polymerase III alpha subunit", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_W4V224" ], "nt": "GTGTTTGTGCGTACCACAGGGGAAATGCTCAATGAAATGAGTTTTTTAGGCGAACAATTAGCTTACGAGTTAGTGGTCGAAAACACCAATAAGTTAGCGAAACAACTCACCGCTGATGACCTGGTACCAGTGCAAACCAAACTCCAACCACCAGTAATTGAAGGTTCTAACGAAAACCTGGCAGCCAAAACCTGGAGTCAAGCCAAGGCCATTTACGGTGATCCCTTACCTAAGTTAATAGAACAACGGATCCAGGAAGAGTTAAAAGCGATCATTGACAATGGCTTTGGCATTATTTACTGGATTTCCCATTTACTAGTCAAACAATCGGTTCAAGATGGTTACTTTGTTGGACCGCGGGGTTCAATTGGTTCTTCGTTGGTAGCTAACCTGATTGGTATCTCGGAAATTAATCCCTTAGTGCCGCATTATCTCTGTGAGAGCTGTCAGTACTTTGAGGTCAACGAAGAGGTCGATGATGGGTATGACCTCATGGTAAGGGATTGTCCAAAGTGTGGTGCCAAAGCAGCCTTTAAGGGTGATGGTCATAACATTCCCTTTGCTACCTTTATGGGTTTTGCTGGGGACAAGATTCCCGATATTGACTTAAACTTTTCCAGTGAGTACCAAGCCAAAGCCCATGCTTATGTGAGGGAACTGTTTGGTGAACAGTACACCTTCCGTGCTGGCACAATTGCGACAGTGGCCGAAAAAACGGCTTACGGGTATGCGCGCAACTACTTTGAAATTATTAAACAAACCGAATTGGCCACCGCTCCCGAAATTGAACGCTTTAAGCAAAAGTTAGTCGGGATTAAACGCACGACCGGCCAACACCCCGGGGGGATTATGATCTTCCCCAACCACAAATCGGTATATGAGTTTACCCCGTGTGGTTATCCAGCTGATGATACTTCCAGTGATTGA", "aa": "MFVRTTGEMLNEMSFLGEQLAYELVVENTNKLAKQLTADDLVPVQTKLQPPVIEGSNENLAAKTWSQAKAIYGDPLPKLIEQRIQEELKAIIDNGFGIIYWISHLLVKQSVQDGYFVGPRGSIGSSLVANLIGISEINPLVPHYLCESCQYFEVNEEVDDGYDLMVRDCPKCGAKAAFKGDGHNIPFATFMGFAGDKIPDIDLNFSSEYQAKAHAYVRELFGEQYTFRAGTIATVAEKTAYGYARNYFEIIKQTELATAPEIERFKQKLVGIKRTTGQHPGGIMIFPNHKSVYEFTPCGYPADDTSSD", "aa_hexdigest": "0439081c021dcb698a9a430eda5055fe", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_W4V224", "query_cov": 0.8798701298701299, "subject_cov": 0.8187311178247734, "identity": 0.513, "score": 292.0, "evalue": 7.75e-95, "valid": false, "product": "DNA polymerase III alpha subunit", "uniref50_id": "UniRef50_W4V224" }, "pscc": { "uniref50_id": "UniRef50_W4V224", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_W4V224" ], "product": "DNA polymerase III alpha subunit" }, "genes": [], "id": "APKECPOBEN_108", "locus": "GCA002090215_00067" }, { "type": "cds", "sequence": "contig_1", "start": 38875, "stop": 39666, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13482.12", "PFAM:PF00929.31" ], "nt": "ATGGTAGCCCATAACGGTTTGAGTTTTGACTTGCCCTTTTTACAAACCCAGTTTGAAAAATACAACATTGCGCCACTAACCAATCCCTTAATTGATACCTTAGCCTTATCCTGGGCCTTAAATCCCGGGTTTGCCTCCCATACGTTGAGCAATATTTGTGCCAAGCTCAAGTTTGACTTTGATGATGAACGATTGCACCGAGCGGATTATGATACCCAGGCCTTAAAAAAGGTCTTTGACTACTTCAAGGAACAGGTCGAACTCATGGGGATTACTAATCTCGAGCAGTTAGACCAAGAGTTAAACCAGCAGTGCCACTTTGAACTGCTCAAGCGCACTTTTACCAATACCGGCATTATCTACATTAAGAGCCAAAGTGGGTTTGCTAAACTGTATGAACTGTTATCAATTGCGCTCACCGATAACAACGCTACTCGACCCCTAGTGTTAACTTCAACACTCCAAAAGTTTGCTAAATCCTTTGTCATTACTGATAACCCGGTGCAAGGTGATATCTTTAAAGCGGCATTGACTAAACCGCTTAAAGAATTAGAAGCGGCCATTAAAAGAGTTGACTTTGTTTTAATTGCCCCACCTGGTGCTTATGCTGGTTACACCATCAGGGAAGGTTTGAAAAAAGAAGCCATTCCTAACGCAATTAAGTTAGTGGTGGACACGGCCCAAAGACTCAATAAGTTAGTGGCAGTTGCCTCGGATGCTTACTTTATTCACCCCTGGGAGAATGAGTACTATAAGGCCATAGTTTGTGCCAAGGGTCTGGGTGGGCGTTGA", "aa": "MVAHNGLSFDLPFLQTQFEKYNIAPLTNPLIDTLALSWALNPGFASHTLSNICAKLKFDFDDERLHRADYDTQALKKVFDYFKEQVELMGITNLEQLDQELNQQCHFELLKRTFTNTGIIYIKSQSGFAKLYELLSIALTDNNATRPLVLTSTLQKFAKSFVITDNPVQGDIFKAALTKPLKELEAAIKRVDFVLIAPPGAYAGYTIREGLKKEAIPNAIKLVVDTAQRLNKLVAVASDAYFIHPWENEYYKAIVCAKGLGGR", "aa_hexdigest": "48762d8f6414b42a31022eaa2dfded17", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13482.12", "name": "RNase_H_2", "length": 65, "aa_cov": 0.24714828897338403, "hmm_cov": 0.9538461538461539, "evalue": 1.0335586866986472e-06, "score": 24.24502944946289, "start": 1, "stop": 65 }, { "id": "PF00929.31", "name": "RNase_T", "length": 81, "aa_cov": 0.30038022813688214, "hmm_cov": 0.9629629629629629, "evalue": 2.291300648310531e-15, "score": 52.98467254638672, "start": 1, "stop": 79 } ], "seq_stats": { "molecular_weight": 29565.72880000001, "isoelectric_point": 6.961889839172363 }, "id": "APKECPOBEN_109", "locus": "GCA002090215_00068" }, { "type": "cds", "sequence": "contig_1", "start": 39682, "stop": 40143, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00929.31" ], "nt": "ATGAGTGCCTTTGATGGCATTAACAGTGTCCAGGAATACGCCCAGTTTGCCAAAGAACGCGATTGGAAGACAATTGCTGTCACTGACAAGGACAACATTCACATTTATCCCACTTTATATGAAGTGGCCAAAAAGTACGGCTTGAAGGCCATTTATGGTTTGGAATGTAACCTCATTGATGATCACATTAAGATAGTTAGCAATCCTGATAAAACCAAACTTAAGGATGCGACCTTTGTCATCTTCGACATTGAAACAACTGGGTTACACGGTCGGTATGACTCGGTGATTGAGTTTGCGGGAATTAAGGTCAAACATAACCGTGAAGTGGAACGAATGCAGTTCTTTCTCAAGATTGATGGACCCTTACCTGCAGCAGTTACCGAAATTACCAAGATTACCCAAGCACAACTAGAAGATGGGATGGAACAGCAAGCGGGGTTGGAAAAACTGCGCGCTTGA", "aa": "MSAFDGINSVQEYAQFAKERDWKTIAVTDKDNIHIYPTLYEVAKKYGLKAIYGLECNLIDDHIKIVSNPDKTKLKDATFVIFDIETTGLHGRYDSVIEFAGIKVKHNREVERMQFFLKIDGPLPAAVTEITKITQAQLEDGMEQQAGLEKLRA", "aa_hexdigest": "d2a265eba449a44e251e5e808abb06eb", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00929.31", "name": "RNase_T", "length": 77, "aa_cov": 0.47058823529411764, "hmm_cov": 1.0, "evalue": 5.113923800192882e-17, "score": 58.354957580566406, "start": 80, "stop": 151 } ], "seq_stats": { "molecular_weight": 17379.67629999998, "isoelectric_point": 5.581043434143066 }, "id": "APKECPOBEN_110", "locus": "GCA002090215_00069" }, { "type": "cds", "sequence": "contig_1", "start": 40294, "stop": 40728, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTCCTACAGTAACCATGAACTGAAAGCTAACTGCCAGTCCCAAGAATTAGACCAATGGTTAATTGAACAACGCCAAGCCTTCATTAAGTGGATGCACCAAGCTGGGTTTACCCACTTTGGTTTTGTCTCGTTGTTTAATCCACCAGCGGAAAAACAACTAAAAGTGAAATCAATGAAGGTATCCAAGTACGACAAGCAGTTTGAAACCGAGGTCTTTAGTACGGAATTTGTCCCCATTCACAAGATTAACCAACAAATGGATGAGATCAAACTAATGGGGCAAATCTTTGAGTTAAAGGACTTTCCTGGGTACAACAACCTCCGTAATACCCTTAATATTTATGTCACTGATTTCCAACTAGGTGGTTCGTTAATTTTGAAGTGGTTTTACAAGGATCCCAAAACAATTGAGGGCATTAAGATTGGTACCTGA", "aa": "MSYSNHELKANCQSQELDQWLIEQRQAFIKWMHQAGFTHFGFVSLFNPPAEKQLKVKSMKVSKYDKQFETEVFSTEFVPIHKINQQMDEIKLMGQIFELKDFPGYNNLRNTLNIYVTDFQLGGSLILKWFYKDPKTIEGIKIGT", "aa_hexdigest": "b3503883c46a51e63e9243ff970f14c7", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16961.322699999993, "isoelectric_point": 7.820482444763185 }, "id": "APKECPOBEN_111", "locus": "GCA002090215_00070" }, { "type": "cds", "sequence": "contig_1", "start": 41408, "stop": 42559, "strand": "+", "frame": 2, "gene": null, "product": "putative protein MPN_035", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75078", "UniRef:UniRef90_P75079" ], "nt": "ATGAAAGCGTTCCGAAAATTGTTGCTGGGCCTCGCCTTACCCACAACCATCGGTCCCTTATTAGGCTTATTATTAACTAATACTGATACTGTCAAAAATGAAAGCTTAACTACTGTGCGCCACAGGTCCAGCATTAACCAGGACTTTGATGGCATTTTTCATACCGTTAAGTTATTAGAACCAATCCAGCAAAGTAATGCTGATCCCGCCGCTAGTTTTGCGCTCTTTCAGCAAAAGTTTCCCAACCTAAAACGTGTAGCCAACTCCACCTTAAATACCTTTGATGTCTACAACCTTTTGAGTGGTTGGAAGAGTAGCTTAACAGCGTACCTCAACCAGGTGTTGGCACTCCAACAACGGATTAAAGCAGCTGATCAAATTTTCCCTAACCAAAAGGAAACTTTACCCAAGGATGAGAATCCCAACATCTTTGAAGTGTTAGGTGCATATGGTGGTAAGGGTTTCTTTCCCACGCTAGGATCCAACGGGTTACACCTGCCACCACAGCTGTTCCAGTTCTTCCGTGACTTCCAACTAAGTAGCTTTACCATTAAAGACTTTGAGGTAGCGTTAGTGAGTGAACCGGACATAGTGCAACATGACAAAGTGCGCTATGCCTTTCAGGTGCAATTTAACTTAGTGTTACAATTACAAATTAACCGTAACCCAGTGTTATTTGACTTTAACCTACAGTTAAAGACGAATAACTTTGCCAATCAAACCAGTTTTGATGAATTGTTTAACGAAAAAACTAATCCACTTAACTGGCAGTTCTTTAGCAAGCTTAAAGTTAACCATTTAGTGTATGAAGGCAATGACATAACCCAGTTAGCTAATACCTTATTACAAAGCCAGTTTAATGTCTTACAACTTGATTTAAATAAGTCAATTTACCGCTTGAACTTAAACGCAATGGCACAGCGCTTTGAACACGACTGGGTACAACCGTTGTATGCCAAACGCACGCAGGCCAAAATTGCTTACGAAGCGGAACAAGCACGGATTGCGGCCGAGAACAAACGCAAGGAACTAGAACGGCAAAAGCTGTTAGCAGAATTGAAAGCCAAAGCTGAGCATTACCAAAAGATCAAGCAAGCACGGGAAAACATGCTAAAGGGCTTAAAGAGTATTACTGATTTTGTCAAGTTCTGA", "aa": "MKAFRKLLLGLALPTTIGPLLGLLLTNTDTVKNESLTTVRHRSSINQDFDGIFHTVKLLEPIQQSNADPAASFALFQQKFPNLKRVANSTLNTFDVYNLLSGWKSSLTAYLNQVLALQQRIKAADQIFPNQKETLPKDENPNIFEVLGAYGGKGFFPTLGSNGLHLPPQLFQFFRDFQLSSFTIKDFEVALVSEPDIVQHDKVRYAFQVQFNLVLQLQINRNPVLFDFNLQLKTNNFANQTSFDELFNEKTNPLNWQFFSKLKVNHLVYEGNDITQLANTLLQSQFNVLQLDLNKSIYRLNLNAMAQRFEHDWVQPLYAKRTQAKIAYEAEQARIAAENKRKELERQKLLAELKAKAEHYQKIKQARENMLKGLKSITDFVKF", "aa_hexdigest": "47b06f7267b664b8cc264cc9e25d3c55", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 41408, "stop": 42862, "observations": { "mutation": [ 42557, 42665, 42731 ], "stop": [ 42557, 42665, 42731 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75079", "query_cov": 1.0, "subject_cov": 0.575075075075075, "identity": 1.0, "score": 740.0, "evalue": 5.56e-265, "gene_start": 1, "gene_end": 383, "reference_start": 1, "reference_end": 383, "reference_sequence": "MKAFRKLLLGLALPTTIGPLLGLLLTNTDTVKNESLTTVRHRSSINQDFDGIFHTVKLLEPIQQSNADPAASFALFQQKFPNLKRVANSTLNTFDVYNLLSGWKSSLTAYLNQVLALQQRIKAADQIFPNQKETLPKDENPNIFEVLGAYGGKGFFPTLGSNGLHLPPQLFQFFRDFQLSSFTIKDFEVALVSEPDIVQHDKVRYAFQVQFNLVLQLQINRNPVLFDFNLQLKTNNFANQTSFDELFNEKTNPLNWQFFSKLKVNHLVYEGNDITQLANTLLQSQFNVLQLDLNKSIYRLNLNAMAQRFEHDWVQPLYAKRTQAKIAYEAEQARIAAENKRKELERQKLLAELKAKAEHYQKIKQARENMLKGLKSITDFVKFWKSPDRLLVGFNKQDDITTRAGVYKALQIAYANYPQWTFYLTLQGWKNGSELLLKRPSWTNLLSDIHFQKAFNLKNTVSEQTLGAATLPGYGYYGLRMSDWLRWALGYYSYIHMGVPQNVQTKFTGTPDNEQQVWIANEPFEWNKHYGVGPKYKDKAYRFNMEISFELEGWIAVKWWAWAFKGSIPGNWKGKLKVTHLFDGMVPVWELGPVNTHLPQYSFTDQQQLLFVPHSIQKIEAIGADKGINDLLKTQNLHNLERLSYESTNPIDLISYLLYAIQYIKV" }, "paralog": false, "identity": 0.993801652892562, "score": 952.0, "evalue": 0.0, "description": "Internal stop codon at 42557, 42665, 42731. Nonsense mutation around 42557, 42665, 42731.", "psc": { "uniref90_id": "UniRef90_P75079", "product": "Uncharacterized protein MPN_035", "uniref50_id": "UniRef50_P75078", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75079", "UniRef:UniRef50_P75078" ] }, "pscc": { "uniref50_id": "UniRef50_P75078", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75078" ], "product": "Uncharacterized protein MPN_036" } }, "id": "APKECPOBEN_112", "locus": "GCA002090215_00071" }, { "type": "cds", "sequence": "contig_1", "start": 42848, "stop": 43069, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03072.19" ], "nt": "ATGAGTGACTGGTTGCGCTGGGCTTTGGGCTATTACTCCTATATCCATATGGGCGTACCGCAAAATGTACAGACCAAGTTTACGGGCACCCCAGATAATGAACAACAAGTCTGGATTGCTAACGAACCGTTTGAATGGAACAAACATTACGGTGTTGGTCCTAAATACAAGGATAAGGCCTACCGCTTTAACATGGAAATTAGTTTTGAACTAGAGGGTTGA", "aa": "MSDWLRWALGYYSYIHMGVPQNVQTKFTGTPDNEQQVWIANEPFEWNKHYGVGPKYKDKAYRFNMEISFELEG", "aa_hexdigest": "84fb77351fc69cd429b6d2c85d627e64", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03072.19", "name": "DUF237", "length": 69, "aa_cov": 0.9452054794520548, "hmm_cov": 0.9420289855072463, "evalue": 3.822880599082573e-19, "score": 64.58252716064453, "start": 5, "stop": 73 } ], "seq_stats": { "molecular_weight": 8738.680300000002, "isoelectric_point": 5.5347196578979485 }, "id": "APKECPOBEN_113", "locus": "GCA002090215_00072" }, { "type": "cds", "sequence": "contig_1", "start": 43160, "stop": 43408, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03072.19" ], "nt": "ATGGTACCAGTGTGGGAATTGGGCCCAGTCAATACCCATTTACCCCAATACAGCTTTACCGATCAGCAACAACTGTTGTTTGTACCCCACTCCATTCAAAAGATTGAAGCGATTGGTGCGGACAAAGGTATAAACGATCTTTTAAAAACCCAAAACCTCCATAACCTAGAGCGCTTGTCATACGAATCAACTAACCCAATTGACCTCATTTCTTACCTCTTGTACGCGATCCAGTACATTAAGGTTTAG", "aa": "MVPVWELGPVNTHLPQYSFTDQQQLLFVPHSIQKIEAIGADKGINDLLKTQNLHNLERLSYESTNPIDLISYLLYAIQYIKV", "aa_hexdigest": "7edf4e6e1b6f8935ebc8151ddee24830", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03072.19", "name": "DUF237", "length": 36, "aa_cov": 0.43902439024390244, "hmm_cov": 1.0, "evalue": 6.708465983575555e-12, "score": 41.1209716796875, "start": 2, "stop": 37 } ], "seq_stats": { "molecular_weight": 9438.723299999994, "isoelectric_point": 5.375115776062012 }, "id": "APKECPOBEN_114", "locus": "GCA002090215_00073" }, { "type": "cds", "sequence": "contig_1", "start": 43580, "stop": 43891, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03086.20" ], "nt": "ATGAAAACACTGCACAAGTTGCTGTTGGGGCTTTTCTTACCAGCAATCCTCGGTCCGTTGTTTGGTCTACTAGTTACAAAAACTGATGCTGAATCACCAGTTGCAGCATTACATAAATCGAAATCTTTTAGCAAAAACTTAGATAGTTTCCGCGCCTTATTTGTGGGCGAGGATATTGAGGAACAACTAGCTAAGGTACAACAACAAACACCAGAAGTTAGCTTTGCTAGCTTTCAACAAAAGTTTCCTGATAAGGCCAGTTTAAGAAACGGTTTTCAACCAATTGATGTTTACAACTTTTTAAGTGGTTGA", "aa": "MKTLHKLLLGLFLPAILGPLFGLLVTKTDAESPVAALHKSKSFSKNLDSFRALFVGEDIEEQLAKVQQQTPEVSFASFQQKFPDKASLRNGFQPIDVYNFLSG", "aa_hexdigest": "d59b163263be5cda8d5fc69bf4063f4d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03086.20", "name": "DUF240", "length": 40, "aa_cov": 0.3786407766990291, "hmm_cov": 1.0, "evalue": 1.564439877187064e-11, "score": 39.4932975769043, "start": 65, "stop": 103 } ], "seq_stats": { "molecular_weight": 11411.05629999999, "isoelectric_point": 8.027168083190919 }, "id": "APKECPOBEN_115", "locus": "GCA002090215_00074" }, { "type": "cds", "sequence": "contig_1", "start": 43913, "stop": 44353, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03086.20" ], "nt": "TTGGCTAAGGTAGTTGAACTGCAAAAGAAGATTAAAGCAGCTGACGATATTTTCCCGAACCAAAAGAAAAATCCCGACAAGAAAGATAACCCAAATGTTTTAGAAGTGTTAGGTGAATACGGTGGAGATGGTTTCTTTCCTACATTAGGTAATAACGGTTTAAACATCCCGGACCAAGTTTTCCAATCCTTTAATAACTTCCAAATTGAGAACTATAAGATTAGTGACTTTAAGGTGGACATTGCCAGTGAACGTGACATTGTCCAACACGACAAGTTCCGTTTTAGTTATGTCGTTAACATTGGTTTAGAGTTAGCACTTTTAGTCAATAAGCGTCCGGTTTACTTTAACTTTAGCTTGGATCTGCGGACCAATAACTTCTCCAACCAAGCCGGTTTTAACGAAATCTTTAATGCGCCTGGTAACGCAGCTACTAACTGA", "aa": "MAKVVELQKKIKAADDIFPNQKKNPDKKDNPNVLEVLGEYGGDGFFPTLGNNGLNIPDQVFQSFNNFQIENYKISDFKVDIASERDIVQHDKFRFSYVVNIGLELALLVNKRPVYFNFSLDLRTNNFSNQAGFNEIFNAPGNAATN", "aa_hexdigest": "e7988e96cf7e736d18cf425a3e5c8a27", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03086.20", "name": "DUF240", "length": 143, "aa_cov": 0.9794520547945206, "hmm_cov": 1.0, "evalue": 1.715899703129408e-66, "score": 219.39715576171875, "start": 2, "stop": 144 } ], "seq_stats": { "molecular_weight": 16523.340499999988, "isoelectric_point": 5.385744667053221 }, "id": "APKECPOBEN_116", "locus": "GCA002090215_00075" }, { "type": "cds", "sequence": "contig_1", "start": 44375, "stop": 44737, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03086.20" ], "nt": "GTGAAACAGTTGAACTATGATGGTAATGACAGTACCCATTTAGCCAATACCCTCTTACAAGATCAGTTCAATACCTTAAACTTAAACTTGCAAAAGTCGATCTATGAGTTGCCATTAACCGAAATGGAAACTAAGTTCCAGCAAGAATATGTGGAACTTTTACTAGCTAAACGTAGAGAGGAAAAACGACTTTGGGATGAAGAACAAAAACGAATTGAAGCAGAACGGAAACAAAAAGAAGCAGAGCTAGCACGAATTCAGCGTGAACTTAAAGAAAAGGCTGAGAAGGATGCGGCGGTTAAACAAGCTCAAACTAACTTAAAAACTGCTCTAAGTAGTGTGGAAGGCTTTGTGAAATTCTGA", "aa": "MKQLNYDGNDSTHLANTLLQDQFNTLNLNLQKSIYELPLTEMETKFQQEYVELLLAKRREEKRLWDEEQKRIEAERKQKEAELARIQRELKEKAEKDAAVKQAQTNLKTALSSVEGFVKF", "aa_hexdigest": "3675678c764867df6e909522df0067d6", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03086.20", "name": "DUF240", "length": 34, "aa_cov": 0.2833333333333333, "hmm_cov": 1.0, "evalue": 1.0939532212925267e-13, "score": 46.548583984375, "start": 2, "stop": 35 } ], "seq_stats": { "molecular_weight": 14185.907099999984, "isoelectric_point": 5.96033000946045 }, "id": "APKECPOBEN_117", "locus": "GCA002090215_00076" }, { "type": "cds", "sequence": "contig_1", "start": 45077, "stop": 45268, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03072.19" ], "nt": "GTGGGTAGTCCACAAAATGTGAAAGCCACCATTGATGGTAATCCAAGTGATACCACCAAAGTATGGATTGCACCAGAAGACTTTAAACAGGGTGTACACATTGGTGTGGGGGAACACTTCAAAGGTAAGGCTTACAAGTTTAAGTTTGAAGTTAGTTTTGAATTAGAAGGCTCGATTGCAGCCCACTGGTGA", "aa": "MGSPQNVKATIDGNPSDTTKVWIAPEDFKQGVHIGVGEHFKGKAYKFKFEVSFELEGSIAAHW", "aa_hexdigest": "4281035f030a6aca9787762f64cddd68", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03072.19", "name": "DUF237", "length": 56, "aa_cov": 0.8888888888888888, "hmm_cov": 0.9821428571428571, "evalue": 1.3601823232900913e-15, "score": 53.08140182495117, "start": 8, "stop": 63 } ], "seq_stats": { "molecular_weight": 6963.751199999998, "isoelectric_point": 6.247423744201661 }, "id": "APKECPOBEN_118", "locus": "GCA002090215_00077" }, { "type": "cds", "sequence": "contig_1", "start": 45735, "stop": 46010, "strand": "-", "frame": 2, "gene": null, "product": "DUF16 family-like protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W8A6", "UniRef:UniRef90_A0AB38W8A6" ], "nt": "GTGAACACTGAATACGTAACGCGTAAAGAGTTTAACGAGTACAAAAAATCAAAAGACCAACGACTTACAAAGATTGAAACTGAGTTAAAGAGTCAGGTTCAAAGGATTCAAGTTGTTGAGGACAAAGTTGATCGACTAACTGAAGTTGTTATCGTTCAAGGCCAACAAATTAAAGAGCTTCAAGTTGAACAAAAAAATCAAGGAGAAACACTTAGATTAATTTTGAAAGCACTTGAAGGCATAAACAGACGTCTAGACAATTTGGAATCTAAATAA", "aa": "MNTEYVTRKEFNEYKKSKDQRLTKIETELKSQVQRIQVVEDKVDRLTEVVIVQGQQIKELQVEQKNQGETLRLILKALEGINRRLDNLESK", "aa_hexdigest": "e8dfd7532a442c75db385b0b6b0427bf", "start_type": "GTG", "rbs_motif": "AACAA", "psc": { "uniref90_id": "UniRef90_A0AB38W8A6", "query_cov": 1.0, "subject_cov": 0.8198198198198198, "identity": 0.9890000000000001, "score": 160.0, "evalue": 1.96e-49, "valid": true, "product": "DUF16 family-like protein", "uniref50_id": "UniRef50_A0AB38W8A6" }, "pscc": { "uniref50_id": "UniRef50_A0AB38W8A6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W8A6" ], "product": "DUF16 family-like protein" }, "genes": [], "id": "APKECPOBEN_119", "locus": "GCA002090215_00078" }, { "type": "cds", "sequence": "contig_1", "start": 46441, "stop": 46791, "strand": "-", "frame": 1, "gene": null, "product": "UPF0134 protein MPN_038", "db_xrefs": [ "RefSeq:WP_010874395.1", "SO:0001217", "UniParc:UPI0000139F43", "UniRef:UniRef100_P75076", "UniRef:UniRef50_P75076", "UniRef:UniRef90_P75076" ], "nt": "TTGTTTAAGAAAAGACTAAATAAAGATAAGATTAATGATTGTTATACATGGGAAGAAGAACTACCTGATGGTAGCTACGACATGGGATTTCATGGCAATTTAAACCATATGGAAAAAGGTAAAAGTGGATACGTCACCCATAAACAACTGGATAAAAAACTAGAAGTGTTCAAACAAGATCTTCTAGTTGAACTTAGTGAAAAATTTGTAACTAAGGAAGAATTCAGAGCTCAGGGCAAGCAAATCAAAGAACTTCAAATTGAGCAAAAGGCTCAAGGCAAGACACTTCAATTAATACTCGAAGCTCTCCAGGGTATAAACAAACGTTTAGACAAATTGGAATCTAAGTAG", "aa": "MFKKRLNKDKINDCYTWEEELPDGSYDMGFHGNLNHMEKGKSGYVTHKQLDKKLEVFKQDLLVELSEKFVTKEEFRAQGKQIKELQIEQKAQGKTLQLILEALQGINKRLDKLESK", "aa_hexdigest": "a093b243fb02ab51931a319ab54c7036", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000139F43", "ncbi_nrp_id": "WP_010874395.1", "uniref100_id": "UniRef100_P75076", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139F43", "RefSeq:WP_010874395.1", "UniRef:UniRef100_P75076" ] }, "ips": { "uniref100_id": "UniRef100_P75076", "uniref90_id": "UniRef90_P75076" }, "psc": { "uniref90_id": "UniRef90_P75076", "product": "UPF0134 protein MPN_038", "uniref50_id": "UniRef50_P75076" }, "pscc": { "uniref50_id": "UniRef50_P75076", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75076" ], "product": "UPF0134 protein MPN_038" }, "genes": [], "id": "APKECPOBEN_120", "locus": "GCA002090215_00079" }, { "type": "cds", "sequence": "contig_1", "start": 47193, "stop": 47513, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000AD341A4", "UniRef:UniRef50_UPI001FCF25EA", "UniRef:UniRef90_UPI000AD341A4", "UniRef:UniRef90_UPI001FCF25EA", "PFAM:PF03086.20" ], "nt": "ATGACAAAACTCCCTAAATTGTTACTGGGGTTAACCTTTTCTGTCTCACTTATACCGTTTTCTAGTTTGCTTATTACTAGTACTGATGTCAATAAGCAACCGGTTCCAACTGCCTTACAACGCACTGGCGCCTCTGCCATTCATGAAGGTAGTTTTCAAACTATTACTTTAGGTCAAAGTTTAATGGAGCAAATTGAACAGCTCCAACAATTTACGCCAGCGCAGCGCTTTACCCAATTTAAAAAGAAATTTCCCAACCAAAAATTACTCAGTCAAAGTGAGTTAAGTCCAGTTGATGTTTACAATTTTTTAAGTGGTTGA", "aa": "MTKLPKLLLGLTFSVSLIPFSSLLITSTDVNKQPVPTALQRTGASAIHEGSFQTITLGQSLMEQIEQLQQFTPAQRFTQFKKKFPNQKLLSQSELSPVDVYNFLSG", "aa_hexdigest": "a54d492bede0e0f831adf1662ae286f0", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001FCF25EA", "query_cov": 0.8207547169811321, "subject_cov": 0.925531914893617, "identity": 1.0, "score": 162.0, "evalue": 5.25e-50, "valid": true, "uniref50_id": "UniRef50_UPI001FCF25EA" }, "pscc": { "uniref50_id": "UniRef50_UPI001FCF25EA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001FCF25EA" ] }, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 47193, "stop": 47751, "observations": { "insertion": [ 47727 ], "mutation": [ 47511 ], "stop": [ 47511 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000AD341A4", "query_cov": 1.0, "subject_cov": 0.572972972972973, "identity": 1.0, "score": 198.0, "evalue": 3.19e-63, "gene_start": 1, "gene_end": 106, "reference_start": 1, "reference_end": 106, "reference_sequence": "MTKLPKLLLGLTFSVSLIPFSSLLITSTDVNKQPVPTALQRTGASAIHEGSFQTITLGQSLMEQIEQLQQFTPAQRFTQFKKKFPNQKLLSQSELSPVDVYNFLSGWQGALVSFLDRVIKLQGKVKEANEIFNPNVGDQIVLPKKENPNVLEVLGEYNGFGFFPTLGKNGLNLPQQIFKTLLTLK" }, "paralog": false, "identity": 0.9891891891891892, "score": 350.0, "evalue": 1.53e-125, "description": "Internal stop codon at 47511. Frameshift due to insertion around 47727. Nonsense mutation around 47511.", "psc": { "uniref90_id": "UniRef90_UPI000AD341A4", "uniref50_id": "UniRef50_UPI000AD341A4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000AD341A4", "UniRef:UniRef50_UPI000AD341A4" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000AD341A4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000AD341A4" ] } }, "hypothetical": true, "pfams": [ { "id": "PF03086.20", "name": "DUF240", "length": 41, "aa_cov": 0.3867924528301887, "hmm_cov": 1.0, "evalue": 1.4363993359989166e-12, "score": 42.88805389404297, "start": 66, "stop": 106 } ], "seq_stats": { "molecular_weight": 11771.48239999999, "isoelectric_point": 9.52193202972412 }, "id": "APKECPOBEN_121", "locus": "GCA002090215_00080" }, { "type": "cds", "sequence": "contig_1", "start": 47523, "stop": 47981, "strand": "+", "frame": 3, "gene": null, "product": "putative protein MPN_039", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75075", "UniRef:UniRef90_P75075" ], "nt": "TTGGTCTCTTTCTTGGATAGAGTAATTAAATTACAAGGCAAGGTTAAGGAAGCAAATGAAATTTTTAATCCAAATGTAGGTGATCAAATAGTTTTACCTAAAAAGGAAAACCCGAATGTTTTGGAAGTATTGGGCGAGTATAACGGTTTTGGTTTCTTCCCTACATTGGGTAAAAACGGATTAAACCTACCACAGCAAATCTTTGAAAACTTTACTGACTTTAAAGTAGAGAGTTACCAAATTAACGATTTCAAGGTAAGTCTAGTGGGCGAGCGTGACATTATTAAAAACGACAAAGTCCGTTTTTCTTACGCGGTGCAAATACCACTCAACTTAGAACTGCTTGTTAACAACCAAAAGGTTACTTTCAATATTACGGTTGATTTGAGAACCAATAACTTTTCTACCCAAGAGACTTTCAATGATTTATTCAACAATGGAACTGCACCAACCAACTGA", "aa": "MVSFLDRVIKLQGKVKEANEIFNPNVGDQIVLPKKENPNVLEVLGEYNGFGFFPTLGKNGLNLPQQIFENFTDFKVESYQINDFKVSLVGERDIIKNDKVRFSYAVQIPLNLELLVNNQKVTFNITVDLRTNNFSTQETFNDLFNNGTAPTN", "aa_hexdigest": "49ae170815a8e828560d47f96ed40d8b", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 47223, "stop": 48209, "observations": { "mutation": [ 47511, 47979 ], "stop": [ 47511, 47979 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75075", "query_cov": 1.0, "subject_cov": 0.44970414201183434, "identity": 0.993, "score": 301.0, "evalue": 5.47e-101, "gene_start": 1, "gene_end": 152, "reference_start": 111, "reference_end": 262, "reference_sequence": "MTKLPKLLLGLTFSVSLIPFSSLLITSTDVNKQPVPTALQRTGASAIHEGSFQTITLGQSLMEQIEQLQQFTPAQRFTQFKKKFPNQKLLSQSELSPVDVYNFLSGWQDALVSFLDRVIKLQGKVKEANEIFNPNVGDQIVLPKKENPNVLEVLGEYNGFGFFPTLGKNGLNLPQQIFENFTDFKVESYQINDFKVSLVGERDIIKNDKVRFSYAVQIPLNLELLVNNQKVTFNITVDLRTNNFSTQETFNDLFNNGTAPTNWQFFSRIKVNKLHYDQTDATHLANTLLQDQFNALNLDLQKSIYDLNLDDLEERFEEEYAKPLREKRTQQKKEWEEE" }, "paralog": false, "identity": 0.9939024390243902, "score": 643.0, "evalue": 6.15e-238, "description": "Internal stop codon at 47511, 47979. Nonsense mutation around 47511, 47979.", "psc": { "uniref90_id": "UniRef90_P75075", "product": "Uncharacterized protein MPN_039", "uniref50_id": "UniRef50_P75075", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75075", "UniRef:UniRef50_P75075" ] }, "pscc": { "uniref50_id": "UniRef50_P75075", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75075" ], "product": "Uncharacterized protein MPN_039" } }, "id": "APKECPOBEN_122", "locus": "GCA002090215_00081" }, { "type": "cds", "sequence": "contig_1", "start": 48012, "stop": 48209, "strand": "+", "frame": 3, "gene": null, "product": "DUF240 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000AF9F4C5", "UniRef:UniRef90_UPI000AF9F4C5" ], "nt": "TTGCACTATGACCAAACTGATGCCACCCATTTAGCTAACACCCTACTACAAGACCAGTTTAATGCGTTAAACCTTGATTTACAAAAATCGATTTACGACCTTAACCTTGATGATTTAGAAGAAAGGTTTGAAGAGGAATACGCTAAGCCACTTCGTGAAAAAAGAACTCAGCAAAAGAAAGAATGGGAAGAGGAATAG", "aa": "MHYDQTDATHLANTLLQDQFNALNLDLQKSIYDLNLDDLEERFEEEYAKPLREKRTQQKKEWEEE", "aa_hexdigest": "afadafa963528335d54fcd4954ffd2f8", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 47787, "stop": 48209, "observations": { "mutation": [ 47979 ], "stop": [ 47979 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000AF9F4C5", "query_cov": 1.0, "subject_cov": 0.4642857142857143, "identity": 0.9690000000000001, "score": 121.0, "evalue": 5.49e-34, "gene_start": 1, "gene_end": 65, "reference_start": 76, "reference_end": 140, "reference_sequence": "MGERDIIKNDKVRFSYAVQIPLNLELLVNNQKVTFNITVDLRTNNFSTQETFNDLFNNGTAPTNWQFFSRIKVNKLHYDQTDATHLANTLLQDQFNALNLDLQKSIYDLNLDGLEERFEEEYAKPLREKRTQQKKEWEEE" }, "paralog": false, "identity": 0.9785714285714285, "score": 276.0, "evalue": 2.27e-97, "description": "Internal stop codon at 47979. Nonsense mutation around 47979.", "psc": { "uniref90_id": "UniRef90_UPI000AF9F4C5", "product": "DUF240 domain-containing protein", "uniref50_id": "UniRef50_UPI000AF9F4C5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000AF9F4C5", "UniRef:UniRef50_UPI000AF9F4C5" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000AF9F4C5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000AF9F4C5" ], "product": "DUF240 domain-containing protein" } }, "id": "APKECPOBEN_123", "locus": "GCA002090215_00082" }, { "type": "cds", "sequence": "contig_1", "start": 48229, "stop": 48393, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTAAACTGCAAAAAGAAATTGAAGAACAACAAAAGAAACAAAGAGAACAAGCAAAAAACGATGCTGAGCTCAAAAAAGCACAAGATGATCTCAAAGTTGCGCTTAATAGTCTTGACGGTTTTGTTAAATTCTTTACATCAAACGAAGGTCGCTTTAAATTAG", "aa": "MLNCKKKLKNNKRNKENKQKTMLSSKKHKMISKLRLIVLTVLLNSLHQTKVALN", "aa_hexdigest": "736e452a7e7efcc8a81098d493c761a3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6329.709699999999, "isoelectric_point": 11.003479957580566 }, "id": "APKECPOBEN_124", "locus": "GCA002090215_00083" }, { "type": "cds", "sequence": "contig_1", "start": 49008, "stop": 49229, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03072.19" ], "nt": "GTGCGTCCACCACAATACAGTTTTAAGGAACAAGAAAAACAACTTTTATTTGTACCACGTCATGTACAAAAAATTGAAGCTGAAGGTAAACACCTTGAAATAATTAATCCGTTCTTAAAAGATCAACATTTAGATTTTTTTGAACACTACCACCCAGATTTAACGCAGCCTTTAGACATGGTGTCATACCTAATGTATGCCATCGCTGACAAGGTGAAATAA", "aa": "MRPPQYSFKEQEKQLLFVPRHVQKIEAEGKHLEIINPFLKDQHLDFFEHYHPDLTQPLDMVSYLMYAIADKVK", "aa_hexdigest": "f62d6569114a02f37a7eac1ee64640fa", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03072.19", "name": "DUF237", "length": 27, "aa_cov": 0.3698630136986301, "hmm_cov": 0.9629629629629629, "evalue": 2.404518683979577e-07, "score": 26.370834350585938, "start": 2, "stop": 28 } ], "seq_stats": { "molecular_weight": 8811.087799999996, "isoelectric_point": 6.16506404876709 }, "id": "APKECPOBEN_125", "locus": "GCA002090215_00084" }, { "type": "cds", "sequence": "contig_1", "start": 49291, "stop": 49596, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03086.20" ], "nt": "ATGAAAAAACTCCACAAAATTTTATTGGGTTTATCTTTGCCCGCATCTTTGGCGCCCTTATCTGGGTTTATTAGTACTGATGCTGATAACTCACAGTTTTCACTAAAGAAGTCTAGCACTGAAATTAAAGGTACACAATCCATTCACCTGCTTAATTTAGGGGAAAGTTTAACTGAAGAAATTAAAGAGGCACAAAAGCGTACCCCAGAAACTAGTTTTGCCAGTTTCAAACAAAAGTTTCCCAATAAGGAAAGTTTTGTTAAAGGTTTTCAACCAATTGATGTCTACAACTTACTAAGTGGTTGA", "aa": "MKKLHKILLGLSLPASLAPLSGFISTDADNSQFSLKKSSTEIKGTQSIHLLNLGESLTEEIKEAQKRTPETSFASFKQKFPNKESFVKGFQPIDVYNLLSG", "aa_hexdigest": "d06a012651ff44db3191783cbd8a3a38", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03086.20", "name": "DUF240", "length": 40, "aa_cov": 0.38613861386138615, "hmm_cov": 1.0, "evalue": 2.768038545362015e-11, "score": 38.68211364746094, "start": 63, "stop": 101 } ], "seq_stats": { "molecular_weight": 11127.642499999985, "isoelectric_point": 9.260899543762207 }, "id": "APKECPOBEN_126", "locus": "GCA002090215_00085" }, { "type": "cds", "sequence": "contig_1", "start": 49723, "stop": 50064, "strand": "+", "frame": 1, "gene": null, "product": "DUF240 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001FCF16A8", "UniRef:UniRef90_UPI001FCF16A8" ], "nt": "GTGCTAAACGTTTTGGGCTCATATGGTGGTGAAGGGTTCTTCCCTACATTGGGTAAAAACGGGTTGAACCTACCACAACAAATCTTTGAAAACTTCACTGATTTCAAAGTTGTTAGTCATAAAATTCATGACTTCCAAGTAAGTTTGGTAGGCGAACGTGACATTATTAAAAACGACAAAGTCCGTTTTTCTTACGCGGTGCAAATACCACTCAACTTAGAGCTGCTTGTTAACAACCAAAAAGTTACTTTCAACATTACGGTTGATTTGAGAACCAATAATTTCTCTACCCAAGAAACTTTTAACGAGCTGTTCAATAAATGCATTGGCCCCGTTAACTGA", "aa": "MLNVLGSYGGEGFFPTLGKNGLNLPQQIFENFTDFKVVSHKIHDFQVSLVGERDIIKNDKVRFSYAVQIPLNLELLVNNQKVTFNITVDLRTNNFSTQETFNELFNKCIGPVN", "aa_hexdigest": "7b312f3d0292651a87a9cc2e342ab82b", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 49618, "stop": 50289, "observations": { "mutation": [ 50062 ], "stop": [ 50062 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001FCF16A8", "query_cov": 1.0, "subject_cov": 0.5044642857142857, "identity": 0.858, "score": 194.0, "evalue": 4.84e-61, "gene_start": 1, "gene_end": 113, "reference_start": 36, "reference_end": 148, "reference_sequence": "MDRVIKLQGKVKEANEIFNPNVGDQIVLPKKENPNVLEVLGEYNGFGFFPTLGKNGLNLPQQIFENFTDFKVESYQINDFKVSLVGERDIIKNDKVRFSYAVQIPLNLELLVNNQKVTFNITVDLRTNNFSTQETFNDLFNNGTAPTNWQFFSRIKVNKLHYDQTDATHLANTLLQDQFNALNLDLQKSIYDLNLDDLEERFEEEYAKPLREKRTQQKKEWEEE" }, "paralog": false, "identity": 0.7847533632286996, "score": 358.0, "evalue": 1.01e-127, "description": "Internal stop codon at 50062. Nonsense mutation around 50062.", "psc": { "uniref90_id": "UniRef90_UPI001FCF16A8", "product": "DUF240 domain-containing protein", "uniref50_id": "UniRef50_UPI001FCF16A8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001FCF16A8", "UniRef:UniRef50_UPI001FCF16A8" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001FCF16A8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001FCF16A8" ], "product": "DUF240 domain-containing protein" } }, "id": "APKECPOBEN_127", "locus": "GCA002090215_00086" }, { "type": "cds", "sequence": "contig_1", "start": 50095, "stop": 50559, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03086.20" ], "nt": "TTGCACTACGATCAAACTGACGCTACTCATTTAGCTAACACGTTGTTACAAGACCAGTTCAATGCGTTAAATCTTGATTTAGAAAAGTCCATCTATGACTTGGAACTACCTAGATTAGAAGCAGAGTTCCAACAAAAATACGTTGATCCTTTAATTGAGAAAAAGCAAAGACAAAAAGCAGAGTGGGAAGAAGCGGAACGTATCCGTAAGGAAGAAGAGGAAAAACACCAAAAGGAGCTTGAAGAACAACAAAGAATTCAAGCAGAAAAAGCGAAAAATGACGAACAGTTACGTAAAGCGCAAACTGAATTGAAGAAGGCGCTTGGTGGCATCGACTCCTTTGTTGAATTCTTTACGAATAATGATTTACGTCTTAAACTAGGTTACACCAAAGAAGACAACGTTAGAACCCGCGCTGGACTTTTTAGAGCACTAGAAGTTTCCTTTGGTAATTACCGCGCTTGA", "aa": "MHYDQTDATHLANTLLQDQFNALNLDLEKSIYDLELPRLEAEFQQKYVDPLIEKKQRQKAEWEEAERIRKEEEEKHQKELEEQQRIQAEKAKNDEQLRKAQTELKKALGGIDSFVEFFTNNDLRLKLGYTKEDNVRTRAGLFRALEVSFGNYRA", "aa_hexdigest": "6254964efcf692aac7e8de8cd62daa93", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03086.20", "name": "DUF240", "length": 32, "aa_cov": 0.2077922077922078, "hmm_cov": 1.0, "evalue": 5.86430590609277e-12, "score": 40.88821792602539, "start": 1, "stop": 32 } ], "seq_stats": { "molecular_weight": 18288.196500000005, "isoelectric_point": 5.266382789611816 }, "id": "APKECPOBEN_128", "locus": "GCA002090215_00087" }, { "type": "cds", "sequence": "contig_1", "start": 51091, "stop": 51309, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03072.19" ], "nt": "GTGCGTACGCCACAATACAGCTTTAATAAGCAGGAACAAAAGATTCTGTTTGTACCCCATGCTATCCAAAAGATTGCTGCTGAAGGTAGCAACTTAGATTTAATTAATCCATTCTTAAAGGATCAAAAATTAGATGAATTTGAGCACTACCACCCAGATTTAACTAAACCATTAGATTTAGTAGCTTATCTGTTATACGCAATAACCACGAGATCATAG", "aa": "MRTPQYSFNKQEQKILFVPHAIQKIAAEGSNLDLINPFLKDQKLDEFEHYHPDLTKPLDLVAYLLYAITTRS", "aa_hexdigest": "4b877f46287aea38b401af53343dd049", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03072.19", "name": "DUF237", "length": 27, "aa_cov": 0.375, "hmm_cov": 0.9629629629629629, "evalue": 7.829914662012193e-08, "score": 27.948936462402344, "start": 2, "stop": 28 } ], "seq_stats": { "molecular_weight": 8417.559499999992, "isoelectric_point": 6.245775413513184 }, "id": "APKECPOBEN_129", "locus": "GCA002090215_00088" }, { "type": "cds", "sequence": "contig_1", "start": 51633, "stop": 51800, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTAATTTAAGTGATTTTAGTGAATTACCACGTTGGATTGGTGCCGAATTTTTAGGCACATTCTTTTTAATTCTTTTAGGTAACGGTGCTGGTTCGCAGCTAACACTCAATAAGATGTTTGCTAAAGAATCGAAAGCAAAGTTACTAACTGCTGCTTTTGCTTGA", "aa": "MFNLSDFSELPRWIGAEFLGTFFLILLGNGAGSQLTLNKMFAKESKAKLLTAAFA", "aa_hexdigest": "1826edf30e8f6a3c71c7ead8cebc898d", "start_type": "ATG", "rbs_motif": "AAAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6028.004200000001, "isoelectric_point": 8.190466499328615 }, "id": "APKECPOBEN_130", "locus": "GCA002090215_00089" }, { "type": "cds", "sequence": "contig_1", "start": 52068, "stop": 52316, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTTACCCATTGTACTAACCCAGCAATCTTTAACATTCCTCGCAACTTTGCAACTGAGTTTGTAGCAACTAGCGTTTTAATTGCTAGTTTACTGGTGGCTGGTAGTTTTGGTGCCAACCGATTTGACCAGTCACCACGAGGGGTTGTACCAATGTTAGTGGTTACTGGCTTAATTATGTCTTTTGGTGCCGCTACTGGTACTGCAATTAACCCAGCCCGTGATTTAGGTCCGAGAATTGTTTACTGA", "aa": "MVTHCTNPAIFNIPRNFATEFVATSVLIASLLVAGSFGANRFDQSPRGVVPMLVVTGLIMSFGAATGTAINPARDLGPRIVY", "aa_hexdigest": "f85be29309d75ea99616540dc29d6a91", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8622.974299999996, "isoelectric_point": 9.486087608337403 }, "id": "APKECPOBEN_131", "locus": "GCA002090215_00090" }, { "type": "cds", "sequence": "contig_1", "start": 52398, "stop": 52874, "strand": "-", "frame": 2, "gene": "tdk", "product": "thymidine kinase", "db_xrefs": [ "EC:2.7.1.21", "GO:0004797", "GO:0005524", "GO:0005829", "GO:0008270", "GO:0046104", "GO:0071897", "KEGG:K00857", "SO:0001217", "UniRef:UniRef50_P47280", "UniRef:UniRef90_P75070" ], "nt": "GTGAAATCCCGTAACGGTCACAGTGATGAAGCCATTGAAATTAATTCACCTTTAGAAATCTATGACTACCTCACTAAAGATCGTTTTGATGTGGTTGCCATTGATGAAGCCCAATTCTTTTCGAGTGAAATTGTCGAAGTGGTTAAATCACTCAATGATCTCGGCATTAACGTCATTGTTTCTGGTTTAGACACGGACTTCCGCGCTGAACCCTTTGGTAGCATTCCCCAACTATTAGCGATTGCCGACAAGATTTGTAAGTTAGATGCCGTTTGTAATGTCTGTGGTCAGTTAGCCCAACGCACCCAGCGCATAGTGTCCAAGAGCAACGAAACCGTTTTAATTGGTGATATTGAAGCTTATGAACCACGTTGTAAGTTGCACCACCACGTGCAGGGTAAGCCAGTTACAGTTAAGACCAAGCAGTTTAAAGAACGCTTTAAGGAACTAAACGATAACAGCCACAAGCACACCTAA", "aa": "MKSRNGHSDEAIEINSPLEIYDYLTKDRFDVVAIDEAQFFSSEIVEVVKSLNDLGINVIVSGLDTDFRAEPFGSIPQLLAIADKICKLDAVCNVCGQLAQRTQRIVSKSNETVLIGDIEAYEPRCKLHHHVQGKPVTVKTKQFKERFKELNDNSHKHT", "aa_hexdigest": "a4658b649b4d25227492bb57443cb055", "start_type": "GTG", "rbs_motif": null, "genes": [ "tdk" ], "truncated": "3-prime", "pseudogene": { "start": 52398, "stop": 53072, "observations": { "mutation": [ 52940, 53009 ], "stop": [ 52940, 53009 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75070", "query_cov": 1.0, "subject_cov": 0.7053571428571429, "identity": 0.9940000000000001, "score": 313.0, "evalue": 4.15e-107, "gene_start": 1, "gene_end": 158, "reference_start": 67, "reference_end": 224, "reference_sequence": "MFNYNWATMSFSQVFHQSPRGWIEVICGPMFSGKTEELLRKIKRWKLAKIPVIIFKPKIDTRQQHLVKSRNGHSDEAIEINSPLEIYDYLTKDRFDVVAIDEAQFFSSEIVEVVKSLNDLGINVIVSGLDTDFRAEPFGSIPQLLAIADKICKLDAVCNVCGQLAQRTQRIVSKSNETVLIGDIEAYEPRCKLHHHVQGKPVTVKTKQFKERFKELNDNSHKHT" }, "paralog": false, "identity": 0.9866071428571429, "score": 443.0, "evalue": 9.27e-161, "description": "Internal stop codon at 52940, 53009. Nonsense mutation around 52940, 53009.", "psc": { "uniref90_id": "UniRef90_P75070", "gene": "tdk", "product": "thymidine kinase", "ec_ids": [ "2.7.1.21" ], "uniref50_id": "UniRef50_P47280", "kegg_orthology_id": "K00857", "go_ids": [ "GO:0004797", "GO:0005524", "GO:0005829", "GO:0008270", "GO:0046104", "GO:0071897" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75070", "EC:2.7.1.21", "UniRef:UniRef50_P47280", "KEGG:K00857", "GO:0004797", "GO:0005524", "GO:0005829", "GO:0008270", "GO:0046104", "GO:0071897" ] }, "pscc": { "uniref50_id": "UniRef50_P47280", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47280" ], "product": "Thymidine kinase" } }, "id": "APKECPOBEN_132", "locus": "GCA002090215_00091" }, { "type": "cds", "sequence": "contig_1", "start": 53207, "stop": 53602, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00587.30", "PFAM:PF01409.25", "PFAM:PF13393.11" ], "nt": "TTGGAATACGCTGAAGCCTTTAAGCGCACCAATGCCAACGCCGATATTGTCAAGAAGGAGTTGTATGAATTCCATGACAAATCGAACCGGCTGTTAGCCCTGCGTCCCGAGGCTACTGCTGGGATAGTGCGCTTGGTCTGTGAGAACAAACTACTCCAACCACAAAACTACCCGTTAAGGTTGTTTACCATTGGCACCATGTACCGGTATGAACGCCCCCAAAGTAACCGTTACCGCGAGCACTACCAGTTTAGTTGTGAAGTGATAGGTGACACTAATCCAACCGTGTTACTCGACACTTTACTGTTGGGTCATGCCATTATCCAGCAATTGGGAATTGAAGGAGTGATCCTCAAACTTAACAACTTAGGTAACAGTGCTACCATTCAACAATGA", "aa": "MEYAEAFKRTNANADIVKKELYEFHDKSNRLLALRPEATAGIVRLVCENKLLQPQNYPLRLFTIGTMYRYERPQSNRYREHYQFSCEVIGDTNPTVLLDTLLLGHAIIQQLGIEGVILKLNNLGNSATIQQ", "aa_hexdigest": "250c2e5a0a23376fea71c4fd1bd25ebd", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00587.30", "name": "tRNA-synt_2b", "length": 105, "aa_cov": 0.7557251908396947, "hmm_cov": 0.9904761904761905, "evalue": 1.1336024830430587e-12, "score": 43.79168701171875, "start": 22, "stop": 120 }, { "id": "PF01409.25", "name": "tRNA-synt_2d", "length": 74, "aa_cov": 0.549618320610687, "hmm_cov": 0.9324324324324325, "evalue": 1.2869462321743599e-05, "score": 20.253562927246094, "start": 21, "stop": 92 }, { "id": "PF13393.11", "name": "tRNA-synt_His", "length": 123, "aa_cov": 0.9389312977099237, "hmm_cov": 0.9512195121951219, "evalue": 1.9310869709432376e-19, "score": 65.43927001953125, "start": 2, "stop": 124 } ], "seq_stats": { "molecular_weight": 15053.090699999984, "isoelectric_point": 7.7830907821655275 }, "id": "APKECPOBEN_133", "locus": "GCA002090215_00092" }, { "type": "cds", "sequence": "contig_1", "start": 53816, "stop": 54115, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13393.11" ], "nt": "GTGCCGTTTGAGTGGAATCCAACCCTAGTCCGGGGCTTGGACTACTATACTGGGGTAGTGTTTGAATTTGTTAAGGATGACACCACGGTACTAGCTGGTGGGGTGTATGATAACCTAGTGGAAGAGTTAGGTGGTACACCTACTAAAGCGCTGGGTTTTGCTTGTGGCATTGAACGCTCAATTAATTGTTTAAGTGCGGTTAAAAAGCAAGCAATTTTGGCCAATCAACCCCCACGATTACTGGTTATAGGTCTCACCGAAGCAGCGTTGGAAAAGCTGTTACAACTTTCATTGGGTTGA", "aa": "MPFEWNPTLVRGLDYYTGVVFEFVKDDTTVLAGGVYDNLVEELGGTPTKALGFACGIERSINCLSAVKKQAILANQPPRLLVIGLTEAALEKLLQLSLG", "aa_hexdigest": "200267869656c59ceb5b281052b89fee", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13393.11", "name": "tRNA-synt_His", "length": 57, "aa_cov": 0.5555555555555556, "hmm_cov": 0.9824561403508771, "evalue": 7.526025785231652e-13, "score": 43.79265213012695, "start": 4, "stop": 58 } ], "seq_stats": { "molecular_weight": 10683.288399999989, "isoelectric_point": 4.761254692077636 }, "id": "APKECPOBEN_134", "locus": "GCA002090215_00093" }, { "type": "cds", "sequence": "contig_1", "start": 54131, "stop": 54289, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03129.25" ], "nt": "GTGACTATTTATCCTAAAGTTATTAGAATTATCAATGGCATCAGAGCCGCGCAACGGTTAGGTTACCGCTTTTTAGGGGTCATTGGTGGTAATAATTTAGAACAGCAAACCATTACCGTTAAAGACCTGGCTACTGAACAGCAAACAACATACACTTGA", "aa": "MTIYPKVIRIINGIRAAQRLGYRFLGVIGGNNLEQQTITVKDLATEQQTTYT", "aa_hexdigest": "edd2793ff4e7153f8d48217d15fe296e", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03129.25", "name": "HGTP_anticodon", "length": 39, "aa_cov": 0.75, "hmm_cov": 1.0, "evalue": 2.8884279547624926e-07, "score": 26.083879470825195, "start": 12, "stop": 50 } ], "seq_stats": { "molecular_weight": 5868.7206000000015, "isoelectric_point": 9.815069770812986 }, "id": "APKECPOBEN_135", "locus": "GCA002090215_00094" }, { "type": "cds", "sequence": "contig_1", "start": 54291, "stop": 54734, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01336.31" ], "nt": "ATGAATTTAGACAACGCCAAGTGCTTTAAGCAACGGGTTTTTATTGGCAATTTAACTTCCGAGCACCTCAATAAAACCGTTACTATTGCCGGATGGGTCAAACGGATTAAGAAGTTAGGTGAACTTAACTTTGTTATTGTCGGGGACAAGACTAATACTATTCAGGTTACCTGTAAAAATAAGGAACAGGTCAAGTACTTAACCAGGGAAGACTTGGTGATTGTCAAGGGCAAACTGAAACGCTTAGACAGTGTTCGGTTTGAAATTACCAATCCTACTATTACCTTATTTGCCAAGTCCAAAACACCCCCTCTTATCATTGAGGACAAAACTGATGCTTTAGAAGAAGTGCGTTTGCGTTACCGCTACTTAGACTTACGCCGTCCGGTGATGCAAAAGCGCTTGGCGTTACGCCATAAGGTAACTTTAGCCGTCCGGAACTGA", "aa": "MNLDNAKCFKQRVFIGNLTSEHLNKTVTIAGWVKRIKKLGELNFVIVGDKTNTIQVTCKNKEQVKYLTREDLVIVKGKLKRLDSVRFEITNPTITLFAKSKTPPLIIEDKTDALEEVRLRYRYLDLRRPVMQKRLALRHKVTLAVRN", "aa_hexdigest": "4494097b7192b7952a9407b945689d6b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01336.31", "name": "tRNA_anti-codon", "length": 74, "aa_cov": 0.47619047619047616, "hmm_cov": 1.0, "evalue": 1.7912899881308733e-15, "score": 52.1613655090332, "start": 27, "stop": 96 } ], "seq_stats": { "molecular_weight": 17120.06379999999, "isoelectric_point": 10.203362846374514 }, "id": "APKECPOBEN_136", "locus": "GCA002090215_00095" }, { "type": "cds", "sequence": "contig_1", "start": 54744, "stop": 55421, "strand": "+", "frame": 3, "gene": "aspS", "product": "aspartate--tRNA ligase", "db_xrefs": [ "COG:COG0173", "COG:J", "EC:6.1.1.-", "EC:6.1.1.12", "GO:0003676", "GO:0004815", "GO:0005524", "GO:0005737", "GO:0006422", "SO:0001217", "UniRef:UniRef50_P75068", "UniRef:UniRef90_P75068" ], "nt": "ATGGGGTTTATTGAAGTAGAAACACCTACGCTCACGAAGTCCACACCGGAGGGTGCTAGGGACTTTTTGGTACCGGCCAGAATTAGGGAACATAGCTTTTACGCTTTGCCCCAAAGTCCACAAATTTATAAACAGTTATTAATGGTTGGTGGTACGGAAAAGTACTTCCAAATTGCCCATGTTTACCGCGATGAAGACAGCCGCAAAGACCGTCAACCTGAACACACCCAAATTGACTTAGAGGTGGCCTTTTACACCAAGGAAATGGTGATGGATTTAATCCAAAGGTTATTTGTCGATGTCTTTCGCCAAGTGTTAAACATTAAGCTCAAAAAACCGTTTCCAGTGTTAAAGTTTGCTGAAGCCTTTAACCGTTTTGGCAGTGACAAACCGGATTTACGTTATGGTTTCGAACTGGAAGACTGCACTGATTTATTCCAAGACAGTCCGAACCAATTCACTAACTTAATTAATGCTGGCGGCATAGTCGGTGGGATCCAATTACCGAATCTTTATTTAGACGAAGTTTCTTTTAAAGCATTGAGGAAACTAGCTAAGGACAACGGGGTTTCCTTAGAGTTTTACTCTGATAAAGCTAGTTCCCTAAAACAACCCCTGGATTTACCGTTAGCAGGCACCATCTTACTGGTAGCACACAAAAGTAAAACGCAAGCTTGA", "aa": "MGFIEVETPTLTKSTPEGARDFLVPARIREHSFYALPQSPQIYKQLLMVGGTEKYFQIAHVYRDEDSRKDRQPEHTQIDLEVAFYTKEMVMDLIQRLFVDVFRQVLNIKLKKPFPVLKFAEAFNRFGSDKPDLRYGFELEDCTDLFQDSPNQFTNLINAGGIVGGIQLPNLYLDEVSFKALRKLAKDNGVSLEFYSDKASSLKQPLDLPLAGTILLVAHKSKTQA", "aa_hexdigest": "3eb50f3ccdf669972a263758bb687614", "start_type": "ATG", "rbs_motif": null, "genes": [ "aspS" ], "truncated": "both", "pseudogene": { "start": 54444, "stop": 55724, "observations": { "mutation": [ 54732, 55419, 55497 ], "stop": [ 54732, 55419, 55497 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75068", "query_cov": 1.0, "subject_cov": 0.40394973070017953, "identity": 1.0, "score": 448.0, "evalue": 2.88e-154, "gene_start": 1, "gene_end": 225, "reference_start": 152, "reference_end": 376, "reference_sequence": "MNLDNAKCFKQRVFIGNLTSEHLNKTVTIAGWVKRIKKLGELNFVIVGDKTNTIQVTCKNKEQVKYLTREDLVIVKGKLKRLDSVRFEITNPTITLFAKSKTPPLIIEDKTDALEEVRLRYRYLDLRRPVMQKRLALRHKVTLAVRNWLDQMGFIEVETPTLTKSTPEGARDFLVPARIREHSFYALPQSPQIYKQLLMVGGTEKYFQIAHVYRDEDSRKDRQPEHTQIDLEVAFYTKEMVMDLIQRLFVDVFRQVLNIKLKKPFPVLKFAEAFNRFGSDKPDLRYGFELEDCTDLFQDSPNQFTNLINAGGIVGGIQLPNLYLDEVSFKALRKLAKDNGVSLEFYSDKASSLKQPLDLPLAGTILLVAHKSKTQAWTALGAIRNELKYHLNLVKPNQYSFCWIVDFPLYEFDEKEQKWVSAHNMFSNPQPQWLVNFENHKAEALSEQYDLVLNGFELGSGSIRIHDPEVQTRLMQSLGVDPQQFGFVMEAYQYGAPVHAGMGLGLDRLMMIINNVDNIREVMAFPKNAQGIEMHTNAPDQVDIKDITTIWSKHPVK" }, "paralog": false, "identity": 0.9929577464788732, "score": 839.0, "evalue": 8.21e-311, "description": "Internal stop codon at 54732, 55419, 55497. Nonsense mutation around 54732, 55419, 55497.", "psc": { "uniref90_id": "UniRef90_P75068", "gene": "aspS", "product": "aspartate--tRNA ligase", "ec_ids": [ "6.1.1.-", "6.1.1.12" ], "uniref50_id": "UniRef50_P75068", "cog_id": "COG0173", "cog_category": "J", "go_ids": [ "GO:0003676", "GO:0004815", "GO:0005524", "GO:0005737", "GO:0006422" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75068", "EC:6.1.1.-", "EC:6.1.1.12", "UniRef:UniRef50_P75068", "COG:COG0173", "COG:J", "GO:0003676", "GO:0004815", "GO:0005524", "GO:0005737", "GO:0006422" ] }, "pscc": { "uniref50_id": "UniRef50_P75068", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75068" ], "product": "Aspartate--tRNA ligase" } }, "id": "APKECPOBEN_137", "locus": "GCA002090215_00096" }, { "type": "cds", "sequence": "contig_1", "start": 55563, "stop": 55964, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00152.25" ], "nt": "ATGTTCTCCAATCCCCAACCGCAGTGGTTGGTTAACTTTGAAAACCACAAGGCTGAGGCGTTGAGTGAACAGTACGACCTAGTTTTAAATGGGTTTGAACTGGGTAGTGGTTCAATTCGAATCCATGACCCAGAAGTACAAACCCGTTTAATGCAATCTTTAGGGGTGGATCCCCAACAGTTTGGCTTTGTGATGGAGGCTTACCAGTACGGTGCTCCTGTACACGCTGGCATGGGTTTGGGATTAGACCGCTTAATGATGATCATTAACAATGTGGACAACATTAGGGAAGTGATGGCCTTTCCGAAAAATGCCCAAGGGATAGAGATGCACACTAATGCCCCTGATCAGGTTGACATTAAAGACATAACAACAATATGGTCCAAACACCCAGTGAAATAA", "aa": "MFSNPQPQWLVNFENHKAEALSEQYDLVLNGFELGSGSIRIHDPEVQTRLMQSLGVDPQQFGFVMEAYQYGAPVHAGMGLGLDRLMMIINNVDNIREVMAFPKNAQGIEMHTNAPDQVDIKDITTIWSKHPVK", "aa_hexdigest": "23ff0b70d9f50eeac3df90f955241f03", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00152.25", "name": "tRNA-synt_2", "length": 93, "aa_cov": 0.6691729323308271, "hmm_cov": 1.0, "evalue": 3.473443116747729e-33, "score": 110.4085464477539, "start": 16, "stop": 104 } ], "seq_stats": { "molecular_weight": 15009.934499999992, "isoelectric_point": 5.019872093200683 }, "id": "APKECPOBEN_138", "locus": "GCA002090215_00097" }, { "type": "cds", "sequence": "contig_1", "start": 55940, "stop": 56101, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTCCAAACACCCAGTGAAATAAATACCCACCTTAAACACCTCTTGGCCTGTGATGCTTATAAGCTATCACACCGCTTAATGTATCCTAATGACACGACCAACTTATACAGTTGTTTAACAGCCCGGGGTGGTCGTGGTGGTTTTCCAAATTTTGTGTGA", "aa": "MVQTPSEINTHLKHLLACDAYKLSHRLMYPNDTTNLYSCLTARGGRGGFPNFV", "aa_hexdigest": "a54b422c165d3831a2a41c843d2a73cb", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5953.767000000001, "isoelectric_point": 8.770359611511232 }, "id": "APKECPOBEN_139", "locus": "GCA002090215_00098" }, { "type": "cds", "sequence": "contig_1", "start": 56135, "stop": 56446, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTTGGTAACTTTTGTGATAGTGTGTTAGCAGTCCAAAACGATCCTGGGTTGGCCCAAGCGCTCACTGACAAGGTGACCACTGTTTTTGGTGATCCCCAGTTTGGGTTGGAGTTTACCCAGCACATCTGTTATTTAGCTAACTTTTTAAAACAACACCACCAGTTACCCTTAACGGTAAAGATTCACCAGAGTTCTGAGGGTTTAGCCTTCCGTACACCCTTAGTAACTATTACAGGTTCGGACCAAATGGTTCCCGAGTTAGTTTGGTTAGTCAACTATTTTGAAACCGTATTATTGGAAAACATCTGA", "aa": "MFGNFCDSVLAVQNDPGLAQALTDKVTTVFGDPQFGLEFTQHICYLANFLKQHHQLPLTVKIHQSSEGLAFRTPLVTITGSDQMVPELVWLVNYFETVLLENI", "aa_hexdigest": "e8f9f37f8bfa9a4aa3e2a29b4e605e02", "start_type": "GTG", "rbs_motif": "AAAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11552.11759999999, "isoelectric_point": 4.8213903427124025 }, "id": "APKECPOBEN_140", "locus": "GCA002090215_00099" }, { "type": "cds", "sequence": "contig_1", "start": 56447, "stop": 57202, "strand": "+", "frame": 2, "gene": "pncB", "product": "nicotinate phosphoribosyltransferase", "db_xrefs": [ "COG:COG1488", "COG:H", "EC:2.4.2.12", "EC:6.3.4.21", "GO:0004514", "GO:0009435", "GO:0047280", "SO:0001217", "UniRef:UniRef50_P75067", "UniRef:UniRef90_P75067" ], "nt": "TTGTACCAAACTACTTTAACTGTCGCCCAAAGCTTAAAGCTGCTGTTGGAACGCTATGCTAACGAAACCGCGGATAATACTGAATTTACCCACTTTCAGTGTCATGATTTTAGTATGCGTGGTATGAGTAGTCTTCAAAGTGCTTTATATGTAGCTAATGCTCACCTGCAGTACTTTAGTGGCAGTGACACCATTTTAGGCGGTGTAGCTGCTAAATCGATCTTAGCTAGTGAACACTCGGTGATGTGTGCTGATGGTCAAGAGGGTGAATTGAATACCTTTAAACGTTTATTGGAGCAGTTTCCTAACAAAAATCTGTCCTTAGTGATTGATTCTTATGACATGTGGCATGTTCTAGATAACATCTTACCCCAACTCAAGGACTTAGTTTTACAACGACAGGAGAAGCTGTACCTCCGACCTGACTCGGGCAACTTTGAAACCCTTATTTGTCAGGGCAAACGGTTTAACCCCGAGGACAAAACTACCTGGGGAGTAATTGATTACTTAGATTACCACTTTGGTTCCACTGTTAACCAAAAAGGTTATAAGGTTTTGAACCAAAAGTTGGGTATTGTTTATGGCGATGGCATTACCTATGAGCGGATTGAGTACATTTTGGAACAGCTCAAACAACGTGGGTTTTGTTCTTCCAACATTGTCTTTGGGGTTGGTAGTACAACTTACCAAAACCTTAACCGTGATACGTTGGGATTTGTGTATAAGTTGACAGCCATCAAAAAGGGTAATACCTGA", "aa": "MYQTTLTVAQSLKLLLERYANETADNTEFTHFQCHDFSMRGMSSLQSALYVANAHLQYFSGSDTILGGVAAKSILASEHSVMCADGQEGELNTFKRLLEQFPNKNLSLVIDSYDMWHVLDNILPQLKDLVLQRQEKLYLRPDSGNFETLICQGKRFNPEDKTTWGVIDYLDYHFGSTVNQKGYKVLNQKLGIVYGDGITYERIEYILEQLKQRGFCSSNIVFGVGSTTYQNLNRDTLGFVYKLTAIKKGNT", "aa_hexdigest": "4cdaf15c782691db99387183e9b5587d", "start_type": "TTG", "rbs_motif": null, "genes": [ "pncB" ], "truncated": "both", "pseudogene": { "start": 56147, "stop": 57295, "observations": { "mutation": [ 56444, 57200 ], "stop": [ 56444, 57200 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75067", "query_cov": 1.0, "subject_cov": 0.5565410199556541, "identity": 0.996, "score": 507.0, "evalue": 1.38e-178, "gene_start": 1, "gene_end": 251, "reference_start": 170, "reference_end": 420, "reference_sequence": "MVQTPSEINTHLKHLLACDAYKLSHRLMYPNDTTNLYSCLTARGGRGGFPNFVWNHEFAKKIILEVFGNFCDSVLAVQNDPGLAQALTDKVTTVFGDPQFGLEFTQHICYLANFLKQHHQLPLTVKIHQSSEGLAFRTPLVTITGSDQMVPELVWLVNYFETVLLENIWLYQTTLTVAQSLKLLLERYANETADNTEFTHFQCHDFSMRGMSSLQSALYVANAHLQYFSGSDTILGGVAAKSILASEHSVMCADGQEGELNTFKRLLEQFPNKNLSLVIDSYDMWHVLDNILPQLKDLVLQRQEKLYLRPDSGNFETLICQGKRFNPEDKTTWGVIDYLDYHFGSTVNQKGYKVLNQKLGIVYGDGITYERIEYILEQLKQRGFCSSNIVFGVGSTTYQNLNRDTLGFVYKLTAIKKGNTWHDVTKSPITDPTKQSIGGRFDNPNLIQVYG" }, "paralog": false, "identity": 0.9947643979057592, "score": 768.0, "evalue": 4.7e-284, "description": "Internal stop codon at 56444, 57200. Nonsense mutation around 56444, 57200.", "psc": { "uniref90_id": "UniRef90_P75067", "gene": "PncB", "product": "nicotinate phosphoribosyltransferase", "ec_ids": [ "2.4.2.12", "6.3.4.21" ], "uniref50_id": "UniRef50_P75067", "cog_id": "COG1488", "cog_category": "H", "go_ids": [ "GO:0004514", "GO:0009435", "GO:0047280" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75067", "EC:2.4.2.12", "EC:6.3.4.21", "UniRef:UniRef50_P75067", "COG:COG1488", "COG:H", "GO:0004514", "GO:0009435", "GO:0047280" ] }, "pscc": { "uniref50_id": "UniRef50_P75067", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75067" ], "product": "Nicotinamide phosphoribosyltransferase" } }, "id": "APKECPOBEN_141", "locus": "GCA002090215_00100" }, { "type": "cds", "sequence": "contig_1", "start": 57635, "stop": 57754, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGTATTGCGCAACAACAGTAGTTTAAGCCGTGATGGTAAAAGTCGGCTTTTAGAATTTGGTAATGACCTTGGTTTTAGCAAGGAAGAAACTGATGCCTTTGCTAAACAAAAGGATTGA", "aa": "MVLRNNSSLSRDGKSRLLEFGNDLGFSKEETDAFAKQKD", "aa_hexdigest": "1b778b988fca1cdb188e640d95f5ee4a", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4404.826700000001, "isoelectric_point": 6.131188011169433 }, "id": "APKECPOBEN_142", "locus": "GCA002090215_00101" }, { "type": "cds", "sequence": "contig_1", "start": 57884, "stop": 58309, "strand": "+", "frame": 2, "gene": null, "product": "DUF240 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000A7C0B9C", "UniRef:UniRef90_UPI000A7C0B9C" ], "nt": "ATGAACGAGTTGCAAACCAAAGTCAATGAGGCTAATAATATTTTCCCAGTTGAAGCCTTCAAGGTACCTAAGGTTCCAGAAAAACTGTTTGGTTTTGTCAACCAAGGCTTCTTTCCTAAACTAAACCCTAAGGGCTTAAACATTGCTGATAACGTGGCTTCCTTATTTGAACAATACAGCTTAAAGCAAGCAAGTCTTAAGGACTTTGACATATTATTGGAAAAGAAAAACGACATTGTGTTGGAACACAAGGTGCGTTATAACTTTGCTTTGCAATTTAACTTTGAAACCACTTATGTTGGTACTGGTGGGGAAATTAACCTACAGTTTGCACTGCAAGCTTCCACTACTAACTTTAGTAGCTTAGAAGAACTCCAAGCTAGCTTTAGTAAAACAGGCGATAACCTTACTGCCCAATTGTTCTGA", "aa": "MNELQTKVNEANNIFPVEAFKVPKVPEKLFGFVNQGFFPKLNPKGLNIADNVASLFEQYSLKQASLKDFDILLEKKNDIVLEHKVRYNFALQFNFETTYVGTGGEINLQFALQASTTNFSSLEELQASFSKTGDNLTAQLF", "aa_hexdigest": "5ba7808a9df5895b500f696b61ed0ffa", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 57599, "stop": 58339, "observations": { "mutation": [ 57752, 58307 ], "stop": [ 57752, 58307 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000A7C0B9C", "query_cov": 1.0, "subject_cov": 0.5145985401459854, "identity": 0.9790000000000001, "score": 273.0, "evalue": 5.33e-91, "gene_start": 1, "gene_end": 141, "reference_start": 96, "reference_end": 236, "reference_sequence": "MSQHGSSTDPSFVVLRNNSSLSRDGKSRLLEFGNDLGFSKEETDAFAKQKDWNQNFVKFQKQFENKLLDPKNFSLTDVYNLFSGFQQSVTATVQLMNELQTKVNEANNIFPVEAFKVPKVPEKLFGFVNQGFFPKLNPKGLNIADNVASLFEQYSLKQASLKDFDILLEKKNDIVLEHKVRYNFALQFHFETTYIGSGGEINLQFALQASTTNFSSLEELQASFSKTGDNLTAQLFWKPTVTKLVSTLKPLMLVLVGKLTYSLHCKLPLLTLVA" }, "paralog": false, "identity": 0.975609756097561, "score": 473.0, "evalue": 8.02e-172, "description": "Internal stop codon at 57752, 58307. Nonsense mutation around 57752, 58307.", "psc": { "uniref90_id": "UniRef90_UPI000A7C0B9C", "product": "DUF240 domain-containing protein", "uniref50_id": "UniRef50_UPI000A7C0B9C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000A7C0B9C", "UniRef:UniRef50_UPI000A7C0B9C" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000A7C0B9C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000A7C0B9C" ], "product": "DUF240 domain-containing protein" } }, "id": "APKECPOBEN_143", "locus": "GCA002090215_00102" }, { "type": "cds", "sequence": "contig_1", "start": 58508, "stop": 58627, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGCCATTAAGAAAGCTGAAGAAGAGAAAATTAAAAAAGAACTTGAAGAGCAAAAGAAGCGTCAAGAAGAATTAGCAAAACAACAACGAGACAAAGAGGCATTACAAAAGTCACTTTGA", "aa": "MAIKKAEEEKIKKELEEQKKRQEELAKQQRDKEALQKSL", "aa_hexdigest": "39fbb92d0982d849d79b07c8a791bc5e", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4711.3996, "isoelectric_point": 9.04905643463135 }, "id": "APKECPOBEN_144", "locus": "GCA002090215_00103" }, { "type": "cds", "sequence": "contig_1", "start": 58745, "stop": 59014, "strand": "+", "frame": 2, "gene": null, "product": "DUF237 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000AE7A69C", "UniRef:UniRef90_UPI000AE7A69C" ], "nt": "TTGTTAATTGCCGGTTTTAGAAGTGCAATTCAGACTTATTACAAAGATGGCAAAGCAGATCAAAGCCAAAACGCTAAAATTGCTTTTGGCGAAAAGGGTATTCAATTCCCTAAATCTGGCCCAGGACTTGACGGTATTTTCATGTCAGATTTCCTTCGTGGTAACCTTACTGGCAACGCTCACTTTGATTTAAAACTTAAAAAAGTTGAAGTTAAAAACACACAAGGAAAAGACGCACAAGGCAATGATAAAAAAGCCTCCATTAATTGA", "aa": "MLIAGFRSAIQTYYKDGKADQSQNAKIAFGEKGIQFPKSGPGLDGIFMSDFLRGNLTGNAHFDLKLKKVEVKNTQGKDAQGNDKKASIN", "aa_hexdigest": "4fee2ed8116e4876e8dbdb85b3183d26", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 58745, "stop": 59317, "observations": { "mutation": [ 59012, 59162 ], "stop": [ 59012, 59162 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000AE7A69C", "query_cov": 1.0, "subject_cov": 0.43842364532019706, "identity": 1.0, "score": 178.0, "evalue": 3.19e-55, "gene_start": 1, "gene_end": 89, "reference_start": 1, "reference_end": 89, "reference_sequence": "MLIAGFRSAIQTYYKDGKADQSQNAKIAFGEKGIQFPKSGPGLDGIFMSDFLRGNLTGNAHFDLKLKKVEVKNTQGKDAQGNDKKASINWQAKQNNFPFRQVNPWDFSFEVELKYEGSYGLYPGARFLNLFGSLGIPNDWKGEMSVKFVLDGKTPQWIADKPDYPGSLFKFEKNQLKFTPHVKEHVHVENKQFMEKLKESKLA" }, "paralog": false, "identity": 0.9842105263157894, "score": 387.0, "evalue": 7.46e-140, "description": "Internal stop codon at 59012, 59162. Nonsense mutation around 59012, 59162.", "psc": { "uniref90_id": "UniRef90_UPI000AE7A69C", "product": "DUF237 domain-containing protein", "uniref50_id": "UniRef50_UPI000AE7A69C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000AE7A69C", "UniRef:UniRef50_UPI000AE7A69C" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000AE7A69C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000AE7A69C" ], "product": "DUF237 domain-containing protein" } }, "id": "APKECPOBEN_145", "locus": "GCA002090215_00104" }, { "type": "cds", "sequence": "contig_1", "start": 59174, "stop": 59440, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03072.19" ], "nt": "ATGAGCGTTAAGTTTGTGCTCGATGGTAAGACACCTCAATGGATTGCTGATAAACCAGATTACCCAGGTTCCTTGTTTAAATTTGAAAAGAACCAGTTAAAGTTCACTCCCCACGTTAAGGAGCATGTACATGTTGAAAACAAACAATTTATGGAAAAGTTAAAGAGTCAAAACTTGCATAACTTGGAGTTAGCAACTGGTGCTACAAAACCACCTGTTGTGGACTTAGCTAGTTATCTACACTACCTCATTTTGAACCACAAGTAG", "aa": "MSVKFVLDGKTPQWIADKPDYPGSLFKFEKNQLKFTPHVKEHVHVENKQFMEKLKSQNLHNLELATGATKPPVVDLASYLHYLILNHK", "aa_hexdigest": "2d4d89d329b47a8ebacf9cb7f01a604a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03072.19", "name": "DUF237", "length": 48, "aa_cov": 0.5340909090909091, "hmm_cov": 1.0, "evalue": 1.3801294142726661e-17, "score": 59.538230895996094, "start": 1, "stop": 47 } ], "seq_stats": { "molecular_weight": 10197.705899999992, "isoelectric_point": 9.189661979675293 }, "id": "APKECPOBEN_146", "locus": "GCA002090215_00105" }, { "type": "cds", "sequence": "contig_1", "start": 59617, "stop": 60405, "strand": "+", "frame": 1, "gene": null, "product": "DUF240 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000A7C0B9C" ], "nt": "ATGCGCTTTCGGTATAAGGCGTTTCTACTAACTTTATTTGCGTCAACCACAACGCTTACTGGATTCATAATACCTACTTTGAGCCAACACGGTTCGAGTACGGATCCGAGTTTTGTGGCATTGCGCAACAACAGTAGTTTAAGCTGTGATGGTAAAAGTCGGCTTTTAGAATTTGGTAATGACCTTGGTTTTAGCAAGGAAGAAACTGATGTGTTAGCGAAACAGAAAAACTGGGAGCAGAATTACACTAACTTTAAAAAGCAGTTTGAACACAAGCTTTTAGATCCGAAGAGCTTTAGCTTAACTGATGTGTATAACCTCTTTAGTGGTTTTCAACAAAGTGTAGCTGACACGGTTAAGCTAATGAATGAATTGCAAACTCAGGTAAATAAAGCTAACGACATTTACCCAGTGGAAAGCTTTCAAGTGCCTAAGGTACCACAAAAGCTATTTGGATTTGTCGACCAGGGCTTCTTTCCGAAGTTAAACCCGAAGGGCTTGAACATTGCTGATAACGTGGCTTCCTTGTTTGAGAAGTACAGCCTAAAACAAGCAACCCTTAAAGACTTTGACATCGTTTTGGAAAAGAAGAACGACATTGTGTTGGAACACAAGGTGCGTTATAACTTTGCTTTGCAATTTCACTTTGAAACTACATACATAGGTAGCGGTGGCGAAATTAACCTACAGTTTGCACTGCAAGCTTCCACCACTAACTTTAGTAGTTTAGAGGAATTGCAAGCTAGCTTTAGTAAGGTAGGTAATAATTTAACTGCTCAATTGTTCTGA", "aa": "MRFRYKAFLLTLFASTTTLTGFIIPTLSQHGSSTDPSFVALRNNSSLSCDGKSRLLEFGNDLGFSKEETDVLAKQKNWEQNYTNFKKQFEHKLLDPKSFSLTDVYNLFSGFQQSVADTVKLMNELQTQVNKANDIYPVESFQVPKVPQKLFGFVDQGFFPKLNPKGLNIADNVASLFEKYSLKQATLKDFDIVLEKKNDIVLEHKVRYNFALQFHFETTYIGSGGEINLQFALQASTTNFSSLEELQASFSKVGNNLTAQLF", "aa_hexdigest": "ff2a092140a442bd66f18ba54fa99a15", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI000A7C0B9C", "query_cov": 0.9007633587786259, "subject_cov": 0.8613138686131386, "identity": 0.877, "score": 410.0, "evalue": 4.61e-143, "valid": false, "product": "DUF240 domain-containing protein", "uniref50_id": "UniRef50_UPI000A7C0B9C" }, "pscc": { "uniref50_id": "UniRef50_UPI000A7C0B9C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000A7C0B9C" ], "product": "DUF240 domain-containing protein" }, "genes": [], "id": "APKECPOBEN_147", "locus": "GCA002090215_00106" }, { "type": "cds", "sequence": "contig_1", "start": 60832, "stop": 61086, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTTCAATTATTAACTGCTGGAATGCGTGAAGGTATTAAGGAGTATTACAAACATAATAAACCTGACCAAAGCGCTAATGCTAAAAAAGCCTTTGGCCAAAATGGTCTTGCATTTCCGCGAACTGGCTTTGATGGTATCTACATGTCTGACTGGCTTCGTGGTGAATTGAGGAATAAGGGTAACATCAATCTCCATCTCAAACAAAACGAAACCACCGTTAAAAAGATTAGGGATGACATTAGTATCGAATGA", "aa": "MFQLLTAGMREGIKEYYKHNKPDQSANAKKAFGQNGLAFPRTGFDGIYMSDWLRGELRNKGNINLHLKQNETTVKKIRDDISIE", "aa_hexdigest": "52118a002728cc58b72aebcc472f0521", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9658.885399999994, "isoelectric_point": 9.520384788513184 }, "id": "APKECPOBEN_148", "locus": "GCA002090215_00107" }, { "type": "cds", "sequence": "contig_1", "start": 62116, "stop": 62514, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02782.22" ], "nt": "ATGTACTCGGCAGCTGAAAGTGACTTTTATGCTAAGCTAGCACAAAAGGAAGAACAGGAAGTGGTGTTTGTCCCAGCCTTCACTGGTTTAGGCGCACCTTACTGGGATGCTTCAGCAAGAGGCGCCATCTTTGGGATAGAAGCTAATACCAAAAGGGAACATTTAGTAAAAGCTACCTTGGAAGCAATTGCCTTTCAAGCTAATGACCTGATTAAGGCAATGGCTAGTGATTTAAACAGTTCTATTAAAAAAATTAAGGCTGATGGCGGTGCTTGCAATTCCAACTATTTAATGCAATTTCAAGCTGATATTGCTAACTTAGAGGTGATTATTCCTAAGAATGTGGAAACCACTGCCATGGGTGCGGCCTTCCTAGCTGGTTTAGCAGTTAATTATTGA", "aa": "MYSAAESDFYAKLAQKEEQEVVFVPAFTGLGAPYWDASARGAIFGIEANTKREHLVKATLEAIAFQANDLIKAMASDLNSSIKKIKADGGACNSNYLMQFQADIANLEVIIPKNVETTAMGAAFLAGLAVNY", "aa_hexdigest": "38e76e5f49cf0e126bc0b130ac86a101", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02782.22", "name": "FGGY_C", "length": 119, "aa_cov": 0.8939393939393939, "hmm_cov": 1.0, "evalue": 3.449602219905443e-34, "score": 113.78630828857422, "start": 12, "stop": 129 } ], "seq_stats": { "molecular_weight": 14170.988799999977, "isoelectric_point": 4.910002326965332 }, "id": "APKECPOBEN_149", "locus": "GCA002090215_00108" }, { "type": "cds", "sequence": "contig_1", "start": 62536, "stop": 62778, "strand": "-", "frame": 1, "gene": null, "product": "FGGY-family carbohydrate kinase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0030F9964F" ], "nt": "ATGGTACCTATTCGTGGGGTTGCCGGTGACCAACAAGCAGCCTTGTTTGGTCAGCTCTGCGTGGAACCAGCGATGGTTAAAAACACCTATGGTACAGGTTGTTTTATGTTGATGAACATCGGTAACGAACTGAAATACTCCCAACATAACTTATTAACAACTGTAGCTTGGCAGTTAGAAAACCAAAAACCGGTGTATGCCTTAGAAGGTAGTGTTTTTGTGGCTGGCGCAGCCTTAAAGTGA", "aa": "MVPIRGVAGDQQAALFGQLCVEPAMVKNTYGTGCFMLMNIGNELKYSQHNLLTTVAWQLENQKPVYALEGSVFVAGAALK", "aa_hexdigest": "859321ebd9901156384390060af99b60", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0030F9964F", "query_cov": 0.9625, "subject_cov": 0.9625, "identity": 0.5579999999999999, "score": 90.9, "evalue": 1.94e-22, "valid": false, "product": "FGGY-family carbohydrate kinase", "uniref50_id": "UniRef50_UPI0030F9964F" }, "pscc": { "uniref50_id": "UniRef50_UPI0030F9964F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0030F9964F" ], "product": "FGGY-family carbohydrate kinase" }, "genes": [], "id": "APKECPOBEN_150", "locus": "GCA002090215_00109" }, { "type": "cds", "sequence": "contig_1", "start": 63229, "stop": 63318, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAAGTGCTAAAAATAAGGCACAAATTAAATCAAACAACATTGCTGCTGTAGGGATTACCAACCAACGGGAAACCATTGTGTTATGA", "aa": "MQSAKNKAQIKSNNIAAVGITNQRETIVL", "aa_hexdigest": "e00dcb1327047a07f6c13396c7acb3ab", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3141.6012, "isoelectric_point": 10.288976860046386 }, "id": "APKECPOBEN_151", "locus": "GCA002090215_00110" }, { "type": "cds", "sequence": "contig_1", "start": 63337, "stop": 63501, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00370.27" ], "nt": "ATGGATCTAAAACAACAATACATTCTTGCTCTAGATGAAGGAACCAGTTCTTGCCGCACGATTGTGTTTGACAAGGATTTAAACCAAGTAGCGATTGCCCAAAACGAATTTAACCAGTTCTTTCCTAAGAGTGGTTGGGTCGAACAAGATCCCTTAGAAATTTGA", "aa": "MDLKQQYILALDEGTSSCRTIVFDKDLNQVAIAQNEFNQFFPKSGWVEQDPLEI", "aa_hexdigest": "f6d119b44bcbba3c45013df43b23ba03", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A920FAT8", "query_cov": 0.9074074074074074, "subject_cov": 0.8909090909090909, "identity": 0.612, "score": 70.5, "evalue": 4.25e-15, "valid": false, "product": "Carbohydrate kinase FGGY N-terminal domain-containing protein", "uniref50_id": "UniRef50_A0A920FAT8" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00370.27", "name": "FGGY_N", "length": 48, "aa_cov": 0.8888888888888888, "hmm_cov": 1.0, "evalue": 4.81568026323436e-12, "score": 41.419010162353516, "start": 7, "stop": 54 } ], "seq_stats": { "molecular_weight": 6234.928899999999, "isoelectric_point": 4.1646726608276365 }, "id": "APKECPOBEN_152", "locus": "GCA002090215_00111" }, { "type": "cds", "sequence": "contig_1", "start": 63522, "stop": 63944, "strand": "-", "frame": 2, "gene": null, "product": "Glycerol-3-phosphate dehydrogenase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449B2Q2" ], "nt": "GTGGTGGTAACTAATCAGGGCGAACTCCACTTAAACTCAATGGTTTTCATGGTACCAACAATCCATGGTAAAGGGGTAATTGTGTCACCCATGTTAGATGGCAACTTCTTAGTCGGACCCACCGCTTTAGATGGGGTAGACAAGGAAGCTACTCGTTACATTACCAAAGATGCTCCATGTATGTTGACTAAGATTGGCAAACATATGGTGCCAAGTCTAAACATTAATAATGCCTTAATAAGCTTTGCTGGTTCGCGTCCAATTGACAAAGCCACTAATGACTTTATTATTAGAGTAGCTCACAATGACCCTGACTTTGTCATTTTAGGGGGTATGAAATCCCCAGGGCTAACTGCAGCACCAGCCATTGCCCGGGAAGCTGTCAGACTTTTAAATTGGAAATTAACTAAAAAGCCGAACTGA", "aa": "MVVTNQGELHLNSMVFMVPTIHGKGVIVSPMLDGNFLVGPTALDGVDKEATRYITKDAPCMLTKIGKHMVPSLNINNALISFAGSRPIDKATNDFIIRVAHNDPDFVILGGMKSPGLTAAPAIAREAVRLLNWKLTKKPN", "aa_hexdigest": "bdf0e0cdd610ffd7431f4f5a83876723", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A449B2Q2", "query_cov": 0.9, "subject_cov": 0.9264705882352942, "identity": 0.516, "score": 135.0, "evalue": 2.28e-38, "valid": false, "product": "Glycerol-3-phosphate dehydrogenase", "uniref50_id": "UniRef50_A0A449B2Q2" }, "pscc": { "uniref50_id": "UniRef50_A0A449B2Q2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449B2Q2" ], "product": "Glycerol-3-phosphate dehydrogenase" }, "genes": [], "id": "APKECPOBEN_153", "locus": "GCA002090215_00112" }, { "type": "cds", "sequence": "contig_1", "start": 64074, "stop": 64187, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTAGCGAGCAAATGTTTAGCTTTAGCAGCACAGCAAAACAAAGTACAAATTTGCACTAATACTGAGGTTACTAATATTAGTAAGCAAGTTGATGGGACTTATTTAGTTTGA", "aa": "MLASKCLALAAQQNKVQICTNTEVTNISKQVDGTYLV", "aa_hexdigest": "74125aa4a908644cccc537cbd170a1fc", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3997.6159, "isoelectric_point": 7.789279747009277 }, "id": "APKECPOBEN_154", "locus": "GCA002090215_00113" }, { "type": "cds", "sequence": "contig_1", "start": 64200, "stop": 64355, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAGCGCGAACAATTAGAAGTACTAAAGCAAAGAGGCATTGCCAATCAAATTAATCTGGAAGATATTCAAATGCTCTCTAAAGAAGAAACCTTAAAGTTAGAGCCTTACGTTAATCCGGAAATTGTGGCTGGATTAAAAATCGAAGGTAGCTGA", "aa": "MEREQLEVLKQRGIANQINLEDIQMLSKEETLKLEPYVNPEIVAGLKIEGS", "aa_hexdigest": "1dd1e9781e13188fabbe34e3a0893b2d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5838.661899999999, "isoelectric_point": 4.605061149597168 }, "id": "APKECPOBEN_155", "locus": "GCA002090215_00114" }, { "type": "cds", "sequence": "contig_1", "start": 64428, "stop": 64646, "strand": "-", "frame": 2, "gene": null, "product": "FAD-dependent oxidoreductase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00296496E8" ], "nt": "ATGGAAACAAGAGATGTTTTAATAGTTGGCGGTGGTGTAATAGGCTGTGCCACTGCTTATGAATTGAGCCAGTACAAGCTCAAAGTGACACTGGTAGAAAAGCACCATTATTTGGCACAAGAGACTAGCCATGCCAACAGTGGTGTTATCCACACTGGCATTGATCCCAATCCACACAAGTTAACTGCTAAATACAACATTTTGGGTAAAAAGCTCTGA", "aa": "METRDVLIVGGGVIGCATAYELSQYKLKVTLVEKHHYLAQETSHANSGVIHTGIDPNPHKLTAKYNILGKKL", "aa_hexdigest": "5e84aaeae96c19411d08c02039616fdf", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00296496E8", "query_cov": 0.9583333333333334, "subject_cov": 0.92, "identity": 0.522, "score": 76.3, "evalue": 7.58e-17, "valid": false, "product": "FAD-dependent oxidoreductase", "uniref50_id": "UniRef50_UPI00296496E8" }, "pscc": { "uniref50_id": "UniRef50_UPI00296496E8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00296496E8" ], "product": "FAD-dependent oxidoreductase" }, "genes": [], "id": "APKECPOBEN_156", "locus": "GCA002090215_00115" }, { "type": "cds", "sequence": "contig_1", "start": 64829, "stop": 65092, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTGGAAAAGGGTTGGTCAAAAAAAGCCTTTTATTTTTTAGTGGCGTGAGTACTATGGCCGTCTTTTTAGTGTCTTGTGGTGCTACTAGAATTTGGGAATCTTCGATTCAACTGTTAGTTTCCAATGATGAAGCTACATTAGCTGACAAATCCTTTTCTGAGATGTCCTATGAAGGGATTAGAAGGTACTTTAGATCTCAAAAACATATTGAATTACCATCACCAAATAGTTCCCTGTTACAAGACGGCAACGGCCTTTGA", "aa": "MFGKGLVKKSLLFFSGVSTMAVFLVSCGATRIWESSIQLLVSNDEATLADKSFSEMSYEGIRRYFRSQKHIELPSPNSSLLQDGNGL", "aa_hexdigest": "b3ab4bae9a7f52f4dd3e8c7980c210de", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9623.930499999991, "isoelectric_point": 7.894234275817872 }, "id": "APKECPOBEN_157", "locus": "GCA002090215_00116" }, { "type": "cds", "sequence": "contig_1", "start": 65162, "stop": 66175, "strand": "+", "frame": 2, "gene": "med", "product": "putative lipo MG040-like protein", "db_xrefs": [ "COG:COG1744", "COG:T", "GO:0005886", "KEGG:K07335", "SO:0001217", "UniRef:UniRef50_P47286", "UniRef:UniRef90_P75062" ], "nt": "GTGATTGTAGCTACTGGATTTAACCAACAAGAAGCACTACAAGCAATTAGCTCTGATGACCGTCGTTATTTAGCTGATAAAAACGACCTTGCTAAAGTTGGTTTCATTTTTGTCGATGGCCAAATTGAAAAGGAATATAACGTGATTAATAAAACGCCACAATTTAGATCAACCCCACTAAACATTTCATCCGTTGCCTTCCGCAGTGATGATGGTTCCTTCTTAACTGGAGTAGCAACTGCAGTTTATTTGAATTTAAATCAAGATTACTTTTTAAAGAAGAATGGAGCAACCAATAATAGTAGCCAAGATTTAACCGTTTCTGGTTTTGTCGGTGTTCCTATTCCTTCAACCCTTTCTTTTTTAAACGGTTTCCGTTTGGGCATTGCTTATTTTAATGAGGTGATTTACACCCATTTAAGTGATGCTGAAACAACATCTGATAACAAGAGTAATTCTTCAAGTGCTAGCAACTCTGTTTTAGTGCAACTTAAACAGATGCAAGGAAACGACAAAAAGATTAAAAAGATTAAATGGATATCTCCTAAACAAGGAAGCGATAATAATTCGAACCTCAGTATCGATGATCACAAGTCTGGTTCTTTCTCCAGCACTGAACCACGAGCAACAACTATCATTAATAATCTTTTAGACAAGGGTGTGAGTGCCATTATTCCTGTTGCCGGACCGCAAGTAAACTTAGCAGTTAACGAGGTGGCACGTCGCAAAGCGCACACTGCTATTATTGGTGTTGATAGTGCGCAAGAATTATTAGATATTAACCAAGATGCACCTGATAAAGATCAGTTAATCAAAGGTAATAAAAAAATTATTCCTTTCTCTTCTATTAAAGCCTTGGATGTTGCCATTGAAAACATGTTGATTGCCATTCAAAAAGGCAGTGATAATAATGGATATAAAGGATTTGGTTATAACAACATTGGTACGGTTGGAACTAGTTCAGTAGGTATTAGTGAGGCTGGTTATGAATTCTTAATTGATCCAGTTTTTTGA", "aa": "MIVATGFNQQEALQAISSDDRRYLADKNDLAKVGFIFVDGQIEKEYNVINKTPQFRSTPLNISSVAFRSDDGSFLTGVATAVYLNLNQDYFLKKNGATNNSSQDLTVSGFVGVPIPSTLSFLNGFRLGIAYFNEVIYTHLSDAETTSDNKSNSSSASNSVLVQLKQMQGNDKKIKKIKWISPKQGSDNNSNLSIDDHKSGSFSSTEPRATTIINNLLDKGVSAIIPVAGPQVNLAVNEVARRKAHTAIIGVDSAQELLDINQDAPDKDQLIKGNKKIIPFSSIKALDVAIENMLIAIQKGSDNNGYKGFGYNNIGTVGTSSVGISEAGYEFLIDPVF", "aa_hexdigest": "9787d68f39a84e05c118fc11d53cf86d", "start_type": "GTG", "rbs_motif": null, "genes": [ "med" ], "truncated": "both", "pseudogene": { "start": 64862, "stop": 66478, "observations": { "mutation": [ 65090, 66173 ], "stop": [ 65090, 66173 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75062", "query_cov": 1.0, "subject_cov": 0.512937595129376, "identity": 0.997, "score": 639.0, "evalue": 5.84e-226, "gene_start": 1, "gene_end": 337, "reference_start": 112, "reference_end": 448, "reference_sequence": "MFGKGLVKKSLLFFSGVSTMAVFLVSCGATRIWESSIQLLVSNDEATLADKSFSEMSYEGIRRYFRSQKHIELPSPNSSLLQDGNGLWKRPGRTLSDRIATFKNIKNDGSDVIVATGFNQQEALQAISSDDRRYLADKNDLAKVGFIFVDGQIEKEYNVINKTPQFRSTPLNISSVAFRSDDGSFLTGVATAVYLNLNQDYFLKKNGATNNSSQDLTVSGFVGVPIPSTLSFLNGFRLGIAYFNEVIYTHLSDAETTSDNKSNSSSASNSVLVQLKQMQGNDKKIKKIKWISPKQGSDNNSNLSIDDHKSGSFSSTEPRATTIINNLLDKGVSAIIPVAGPQVNLAVNEVARRKAHTAIIGVDSAQELLDINQDAPDKDQLIKGNKKIIPFSSIKALDVAIENMLIAIQKGSDNNGYKGFGYNNIGTVGTSSVGISEAGYEFLIDPVFWKTTQSQGKSMATNMTNLKRLSSDDTNTKKALKEVSTHKNGSDKDGIIGKYSNLLTKKSTTVTAVAQKSMTDNNSGTEQKKNLSEVDTKKKEKESKGKTQSNGQDSGQQNGKETNDIISKYSKLLTMTTMNNKVMSSKKQSSDDNSFKKTSENGDWVIKGDELTKKKSTELPAFAKGADYPTFPTEAVSVINGSTALDGKGFKWSFKQI" }, "paralog": false, "identity": 0.9962825278810409, "score": 1038.0, "evalue": 0.0, "description": "Internal stop codon at 65090, 66173. Nonsense mutation around 65090, 66173.", "psc": { "uniref90_id": "UniRef90_P75062", "gene": "Med", "product": "Uncharacterized lipoprotein MG040 homolog", "uniref50_id": "UniRef50_P47286", "cog_id": "COG1744", "cog_category": "T", "kegg_orthology_id": "K07335", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75062", "UniRef:UniRef50_P47286", "COG:COG1744", "COG:T", "KEGG:K07335", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47286", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47286" ], "product": "Uncharacterized lipoprotein MG040" } }, "id": "APKECPOBEN_158", "locus": "GCA002090215_00117" }, { "type": "cds", "sequence": "contig_1", "start": 66203, "stop": 66640, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCCACAAATATGACTAATTTAAAACGTTTATCATCTGATGATACAAACACTAAGAAAGCATTAAAAGAGGTAAGCACCCATAAAAATGGTAGTGATAAAGATGGGATTATTGGTAAATATTCCAATTTATTAACTAAAAAAAGCACAACAGTAACTGCTGTTGCTCAAAAATCTATGACAGACAATAATAGTGGAACTGAGCAAAAAAAGAATCTTTCTGAAGTAGATACTAAGAAGAAAGAGAAAGAAAGTAAAGGTAAAACTCAAAGTAATGGTCAGGACAGTGGCCAACAAAACGGAAAAGAAACAAACGATATTATTTCTAAGTATTCTAAGCTGTTAACTATGACAACTATGAACAATAAAGTAATGTCATCTAAAAAACAAAGTAGCGATGATAACAGTTTTAAAAAGACTTCAGAAAACGGAGACTGA", "aa": "MATNMTNLKRLSSDDTNTKKALKEVSTHKNGSDKDGIIGKYSNLLTKKSTTVTAVAQKSMTDNNSGTEQKKNLSEVDTKKKEKESKGKTQSNGQDSGQQNGKETNDIISKYSKLLTMTTMNNKVMSSKKQSSDDNSFKKTSENGD", "aa_hexdigest": "3e707f1c1aff50d4d726a2f89400c525", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15895.454799999989, "isoelectric_point": 9.602001762390138 }, "id": "APKECPOBEN_159", "locus": "GCA002090215_00118" }, { "type": "cds", "sequence": "contig_1", "start": 66820, "stop": 67086, "strand": "+", "frame": 1, "gene": "ptsH", "product": "phosphocarrier protein HPr", "db_xrefs": [ "BlastRules:WP_000411080", "BlastRules:WP_010874410", "COG:COG1925", "COG:TG", "GO:0005737", "GO:0009401", "RefSeq:WP_010874410.1", "SO:0001217", "UniParc:UPI00001328F5", "UniRef:UniRef100_P75061", "UniRef:UniRef50_P75061", "UniRef:UniRef90_P75061" ], "nt": "ATGAAGAAGATTCAAGTAGTCGTTAAAGACCCTGTGGGCATTCACGCCCGTCCTGCTAGTATTATTGCTGGTGAGGCTAATAAATTTAAATCGGAATTAAAGCTAGTATCACCAAGCGGCGTTGAAGGTAACATCAAGTCGATCATCAACTTAATGTCTTTAGGCATTAAGCAAAACGACCACATTACCATCAAGGCAGAAGGAACTGATGAAGAAGAGGCACTAAATGCTATTAAAGCAGTTTTAGAAAAACACCAAGTTATTTAA", "aa": "MKKIQVVVKDPVGIHARPASIIAGEANKFKSELKLVSPSGVEGNIKSIINLMSLGIKQNDHITIKAEGTDEEEALNAIKAVLEKHQVI", "aa_hexdigest": "ea7f7abc14674640ebb74226629ff732", "start_type": "ATG", "rbs_motif": "AAAA", "ups": { "uniparc_id": "UPI00001328F5", "ncbi_nrp_id": "WP_010874410.1", "uniref100_id": "UniRef100_P75061", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001328F5", "RefSeq:WP_010874410.1", "UniRef:UniRef100_P75061" ] }, "ips": { "uniref100_id": "UniRef100_P75061", "uniref90_id": "UniRef90_P75061" }, "psc": { "uniref90_id": "UniRef90_P75061", "gene": "ptsH", "product": "Phosphocarrier protein HPr", "uniref50_id": "UniRef50_P75061", "cog_id": "COG1925", "cog_category": "TG", "go_ids": [ "GO:0005737", "GO:0009401" ] }, "pscc": { "uniref50_id": "UniRef50_P75061", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75061" ], "product": "Phosphocarrier protein HPr" }, "expert": [ { "type": "expert_proteins", "source": "BlastRules", "rank": 80, "id": "WP_010874410", "gene": "ptsH", "product": "phosphocarrier protein HPr", "query_cov": 1.0, "subject_cov": 1.0, "identity": 1.0, "score": 168.0, "evalue": 1.27e-56, "db_xrefs": [ "BlastRules:WP_000411080", "BlastRules:WP_010874410" ] } ], "genes": [ "ptsH" ], "id": "APKECPOBEN_160", "locus": "GCA002090215_00119" }, { "type": "cds", "sequence": "contig_1", "start": 67163, "stop": 67288, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTTACTACGACGACGAGGATGAATATGACACTTCTAATTCTGGTGGTGTCGTAGATTTAGCATTTTTAGCACTAATAGTAGTTTTTCTATTTACCGCTTTTATTTCTTGGTTTGGGGCATGA", "aa": "MLYYDDEDEYDTSNSGGVVDLAFLALIVVFLFTAFISWFGA", "aa_hexdigest": "722943f8e320b6775e88410304ac02b3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4616.109799999998, "isoelectric_point": 4.0500284194946286 }, "id": "APKECPOBEN_161", "locus": "GCA002090215_00120" }, { "type": "cds", "sequence": "contig_1", "start": 68028, "stop": 68252, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI000B1E1D42", "UniRef:UniRef100_A0AB38W877", "UniRef:UniRef50_A0AB38W877", "UniRef:UniRef90_A0AB38W877" ], "nt": "GTGTTTCTCAAATATTTCTCTAAGAATGAGATTTTTATTCTTTGGTATTTCCACGTTATTTATCGAAATAATCTCTTTTTATTTCTAGAAAATTACTTTGATTTTTGTCTAAGGATTCTCAACTTAGTCCCTAAATTTTCTCCGCTTTTTAGCGAAGAATATTTCAATTTATTCTTTGATTTTTATCTAAAAACTATCCCCTTAGTTACTTTAAATTTCTGTTAA", "aa": "MFLKYFSKNEIFILWYFHVIYRNNLFLFLENYFDFCLRILNLVPKFSPLFSEEYFNLFFDFYLKTIPLVTLNFC", "aa_hexdigest": "e953e932fffa4f1103799e920d23464a", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000B1E1D42", "uniref100_id": "UniRef100_A0AB38W877", "db_xrefs": [ "SO:0001217", "UniParc:UPI000B1E1D42", "UniRef:UniRef100_A0AB38W877" ] }, "ips": { "uniref100_id": "UniRef100_A0AB38W877", "uniref90_id": "UniRef90_A0AB38W877" }, "psc": { "uniref90_id": "UniRef90_A0AB38W877", "uniref50_id": "UniRef50_A0AB38W877" }, "pscc": { "uniref50_id": "UniRef50_A0AB38W877", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W877" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9324.917199999989, "isoelectric_point": 6.527128410339355 }, "id": "APKECPOBEN_162", "locus": "GCA002090215_00121" }, { "type": "cds", "sequence": "contig_1", "start": 68625, "stop": 68855, "strand": "+", "frame": 3, "gene": null, "product": "putative lipoprotein MPN_054", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P75060", "UniRef:UniRef50_UPI001FCEF156", "UniRef:UniRef90_P75060", "UniRef:UniRef90_UPI001FCEF156" ], "nt": "ATGAAGATTAAATATTTCTTTATTCCCTTGTTTTCTTCAGCTATATTATTTAGTGCCTGTTCTTCTATTCAGTCTGATCTTAGGAATCTTATTAAAGAAACAACTGGCAAAGATTTTGACGTATCTAAATTAATTAAAACCTCTGAAGGAAGAAAAAACTTAATAAATTCTCTCAAAAAGTCTTACGAATCTAAGCCCGATGAAACAGCTAGCTTATTACTAATTGCTTGA", "aa": "MKIKYFFIPLFSSAILFSACSSIQSDLRNLIKETTGKDFDVSKLIKTSEGRKNLINSLKKSYESKPDETASLLLIA", "aa_hexdigest": "c97dab709696ed0a5b41490fb47ad8ea", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001FCEF156", "query_cov": 0.9473684210526315, "subject_cov": 0.9863013698630136, "identity": 0.986, "score": 136.0, "evalue": 9.63e-41, "valid": true, "uniref50_id": "UniRef50_UPI001FCEF156" }, "pscc": { "uniref50_id": "UniRef50_UPI001FCEF156", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001FCEF156" ] }, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 68607, "stop": 68996, "observations": { "mutation": [ 68853 ], "stop": [ 68853 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75060", "query_cov": 1.0, "subject_cov": 0.5891472868217055, "identity": 1.0, "score": 144.0, "evalue": 4.61e-43, "gene_start": 1, "gene_end": 76, "reference_start": 7, "reference_end": 82, "reference_sequence": "RYLSNLMKIKYFFIPLFSSAILFSACSSIQSDLRNLIKETTGKDFDVSKLIKTSEGRKNLINSLKKSYESKPDETASLLLIAWKQSAEMGEIGFDDIKEGGIYTRDNDPFKLEQKVEYFNMEYKILVMF" }, "paralog": false, "identity": 0.9922480620155039, "score": 250.0, "evalue": 2.51e-87, "description": "Internal stop codon at 68853. Nonsense mutation around 68853.", "psc": { "uniref90_id": "UniRef90_P75060", "product": "Uncharacterized lipoprotein MPN_054", "uniref50_id": "UniRef50_P75060", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75060", "UniRef:UniRef50_P75060", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75060", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75060" ], "product": "Uncharacterized lipoprotein MPN_054" } }, "id": "APKECPOBEN_163", "locus": "GCA002090215_00122" }, { "type": "cds", "sequence": "contig_1", "start": 68871, "stop": 68996, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGCGAAATAGGTTTTGATGATATTAAAGAAGGAGGAATTTATACCAGAGACAATGATCCTTTCAAATTGGAACAAAAGGTTGAGTACTTCAATATGGAATACAAAATTTTAGTGATGTTTTAA", "aa": "MGEIGFDDIKEGGIYTRDNDPFKLEQKVEYFNMEYKILVMF", "aa_hexdigest": "43d7734811b693ec99330b72d074c09f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4924.5788999999995, "isoelectric_point": 4.399986076354981 }, "id": "APKECPOBEN_164", "locus": "GCA002090215_00123" }, { "type": "cds", "sequence": "contig_1", "start": 70402, "stop": 71289, "strand": "+", "frame": 1, "gene": "potA", "product": "Spermidine/putrescine import ATP-binding protein PotA", "db_xrefs": [ "COG:COG3839", "COG:G", "EC:7.6.2.11", "GO:0005524", "GO:0005886", "GO:0015417", "GO:0016887", "SO:0001217", "UniRef:UniRef50_P75059", "UniRef:UniRef90_P75059" ], "nt": "TTGAACGAACGTTTTTTAATTGAAATCGAAGGTTTAAACAAAACTTTCGATGATGGTTTTGTATCAGTACGTGATATTAACCTCAAAATTAAGAAGGGCGAATTTATTACGATTCTCGGTCCTTCTGGTTGTGGCAAAACAACTACTCTTAGGTTGTTAGCAGGTTTTGAAGATCCTACTTATGGCAAGATCAAAGTTAATGGTCTTGACATTAAAGACTTGCCCATTCATAAGCGTCCCTTTGCTACTGTCTTCCAAGACTATGCCTTGTTTTCACACTTAACTGTGTACAAGAACATTGCTTATGGCTTGAAGGCAATGTACACAAAGCTGGATCCGATTGATAAGCTAGTGGAACAATACCACCAAAGCTTACTGGACAAGCAACACCGCTTGCACAAGAGGATTGAACGCTTAGAAAAAAGTAATGCTAATCCACAACTGTTAGAACAACTTAAACAAACGGTTGTCGTTCAACAAAAACAGTTTAAACAACAAACAGAGACTTTTAAACAAAAGGAAAATGCTCGACGTGATGCCATTCAACAACGCTTGGTGCAACTTACCAAGGAATGGGAAAGCTTATCAAAGCAGAAGTTGCAACAATTAGAAGCGGAGAAAAAGTTGTTAGATAAAAAGTTTGAACAAACTGAGCGCAAATACCAAAAGGATGCTTGGATGGCAACCCACAGTGAAATGCGTTTAAAGCAGTTCAAACAAGAGGTGTTAGCTTTAAAACAACTAATTAAAACTAAGTTCAAACAAAATGAACCAGTTGACAAGTTGCAGTTAAAGCTGCAAACCTTAAAGCAAAAGTATGCTGCTAAAAGACAAATTGATAAGGAATACGATAAGTTGGTCTTAGCCTACAACAAGAAGGACTATTGA", "aa": "MNERFLIEIEGLNKTFDDGFVSVRDINLKIKKGEFITILGPSGCGKTTTLRLLAGFEDPTYGKIKVNGLDIKDLPIHKRPFATVFQDYALFSHLTVYKNIAYGLKAMYTKLDPIDKLVEQYHQSLLDKQHRLHKRIERLEKSNANPQLLEQLKQTVVVQQKQFKQQTETFKQKENARRDAIQQRLVQLTKEWESLSKQKLQQLEAEKKLLDKKFEQTERKYQKDAWMATHSEMRLKQFKQEVLALKQLIKTKFKQNEPVDKLQLKLQTLKQKYAAKRQIDKEYDKLVLAYNKKDY", "aa_hexdigest": "3c064fba9f648766d04a231e95ea2d8b", "start_type": "TTG", "rbs_motif": "AAAA", "genes": [ "potA" ], "truncated": "3-prime", "pseudogene": { "start": 70402, "stop": 71592, "observations": { "mutation": [ 71287 ], "stop": [ 71287 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75059", "query_cov": 1.0, "subject_cov": 0.5267857142857143, "identity": 1.0, "score": 550.0, "evalue": 2.35e-193, "gene_start": 1, "gene_end": 295, "reference_start": 1, "reference_end": 295, "reference_sequence": "MNERFLIEIEGLNKTFDDGFVSVRDINLKIKKGEFITILGPSGCGKTTTLRLLAGFEDPTYGKIKVNGLDIKDLPIHKRPFATVFQDYALFSHLTVYKNIAYGLKAMYTKLDPIDKLVEQYHQSLLDKQHRLHKRIERLEKSNANPQLLEQLKQTVVVQQKQFKQQTETFKQKENARRDAIQQRLVQLTKEWESLSKQKLQQLEAEKKLLDKKFEQTERKYQKDAWMATHSEMRLKQFKQEVLALKQLIKTKFKQNEPVDKLQLKLQTLKQKYAAKRQIDKEYDKLVLAYNKKDYWTSYWETYSLQQQEAFEKRYLSRKLTKQEQHQKVCAVIELVGLKGNEDKLPEELSGGMKQRVALARSLVIEPDILLLDEPLSALDAKVRKNLQKELQKIHQQSGLTFILVTHDQEEALVLSNRIVVMNEGNILQVGSPADIYDSPKTEWIANFIGQANIFKGTYLGDLKIKLHSGEVIKTDVDNNYVVGKEYKILIRPEDFDIVPKNKGFFNVRVIDKTYKGLLWKITTKLVDDTIVDLESVNDIEVDKTFGVTFDPIDVHLMEV" }, "paralog": false, "identity": 0.9949494949494949, "score": 775.0, "evalue": 5.55e-284, "description": "Internal stop codon at 71287. Nonsense mutation around 71287.", "psc": { "uniref90_id": "UniRef90_P75059", "gene": "potA", "product": "Spermidine/putrescine import ATP-binding protein PotA", "ec_ids": [ "7.6.2.11" ], "uniref50_id": "UniRef50_P75059", "cog_id": "COG3839", "cog_category": "G", "go_ids": [ "GO:0005886", "GO:0015417", "GO:0005524", "GO:0016887" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75059", "EC:7.6.2.11", "UniRef:UniRef50_P75059", "COG:COG3839", "COG:G", "GO:0005886", "GO:0015417", "GO:0005524", "GO:0016887" ] }, "pscc": { "uniref50_id": "UniRef50_P75059", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75059" ], "product": "Spermidine/putrescine import ATP-binding protein PotA" } }, "id": "APKECPOBEN_165", "locus": "GCA002090215_00124" }, { "type": "cds", "sequence": "contig_1", "start": 71386, "stop": 71961, "strand": "+", "frame": 1, "gene": null, "product": "ABC transporter ATP-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F197FDC", "UniRef:UniRef90_UPI001F197FDC" ], "nt": "GTGTGTGCTGTAATTGAGTTAGTTGGTTTAAAGGGTAACGAGGACAAACTCCCAGAAGAATTGTCTGGGGGAATGAAGCAACGGGTAGCTTTAGCGCGTTCCTTGGTAATTGAACCAGACATTCTCTTATTGGATGAACCCTTGTCTGCTTTAGATGCTAAGGTACGTAAAAACTTGCAAAAGGAATTGCAAAAGATTCACCAACAAAGTGGTTTAACCTTTATCTTAGTTACTCACGACCAGGAAGAAGCCTTGGTCTTGTCAAACCGCATTGTGGTAATGAATGAGGGTAACATTCTCCAAGTTGGTAGTCCGGCTGATATTTACGATTCACCCAAAACCGAGTGGATTGCTAACTTCATTGGTCAAGCTAATATCTTTAAGGGTACTTACCTAGGTGACCTTAAGATTAAACTGCACAGCGGTGAAGTGATTAAAACTGATGTGGACAATAACTATGTGGTTGGTAAGGAATACAAAATCCTAATTCGTCCCGAAGACTTTGACATTGTGCCCAAAAACAAAGGCTTCTTTAATGTTCGGGTAATTGATAAAACCTACAAAGGATTACTCTGA", "aa": "MCAVIELVGLKGNEDKLPEELSGGMKQRVALARSLVIEPDILLLDEPLSALDAKVRKNLQKELQKIHQQSGLTFILVTHDQEEALVLSNRIVVMNEGNILQVGSPADIYDSPKTEWIANFIGQANIFKGTYLGDLKIKLHSGEVIKTDVDNNYVVGKEYKILIRPEDFDIVPKNKGFFNVRVIDKTYKGLL", "aa_hexdigest": "c9980fc5d079bbf83c983fbc1d91f765", "start_type": "GTG", "rbs_motif": "AACAA", "genes": [], "truncated": "both", "pseudogene": { "start": 71257, "stop": 72084, "observations": { "mutation": [ 71287, 71959 ], "stop": [ 71287, 71959 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001F197FDC", "query_cov": 0.9842931937172775, "subject_cov": 0.6836363636363636, "identity": 0.883, "score": 337.0, "evalue": 4.74e-115, "gene_start": 4, "gene_end": 191, "reference_start": 47, "reference_end": 234, "reference_sequence": "MVVAYNKKDYWTSYWETYTLQQKEAFEKRYLSRKLTKAEQNKKVSDVIEMVGLKGKEDRLPDELSGGMKQRVALARSLVVEPEILLLDEPLSALDAKVRKNLQKELQQIHKKSGLTFILVTHDQEEALVLSDRIVVMNEGNILQVGNPVDIYDSPKTEWIANFIGQANIFKGTYLGEKKIQLQSGEIIQTDVDNNYVVGKQYKILIRPEDFDLVPENKGFFNVRVIDKNYKGLLWKITTQLKDNTIVDLESVNEVDVNKTFGVLFDPIDVHLMEV" }, "paralog": false, "identity": 0.850909090909091, "score": 473.0, "evalue": 3.95e-171, "description": "Internal stop codon at 71287, 71959. Nonsense mutation around 71287, 71959.", "psc": { "uniref90_id": "UniRef90_UPI001F197FDC", "product": "ABC transporter ATP-binding protein", "uniref50_id": "UniRef50_UPI001F197FDC", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001F197FDC", "UniRef:UniRef50_UPI001F197FDC" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001F197FDC", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F197FDC" ], "product": "ABC transporter ATP-binding protein" } }, "id": "APKECPOBEN_166", "locus": "GCA002090215_00125" }, { "type": "cds", "sequence": "contig_1", "start": 72086, "stop": 72262, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAACTATCGAAAAAATACCTCTTAGCTGTACCATTCTTTGTTTTAATGGTCATCTTCTTTGTAGTACCAATGGCATGGATTATTGTTAGTGGACTCCAAAATGAAAACGGTGCAAGTATAACGGAAAAGTACCAACCCTTAGTTGGTGGTTATTCTTTCTTTCAGAGTTTTTGA", "aa": "MKLSKKYLLAVPFFVLMVIFFVVPMAWIIVSGLQNENGASITEKYQPLVGGYSFFQSF", "aa_hexdigest": "175b6ae90ee0fa8116a19eac8dbc2b8f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6595.827299999999, "isoelectric_point": 9.224861717224119 }, "id": "APKECPOBEN_167", "locus": "GCA002090215_00126" }, { "type": "cds", "sequence": "contig_1", "start": 72284, "stop": 72946, "strand": "+", "frame": 2, "gene": "potB", "product": "Spermidine/putrescine transport system permease PotB-like protein", "db_xrefs": [ "COG:COG1176", "COG:E", "GO:0005886", "GO:0055085", "SO:0001217", "UniRef:UniRef50_P47289", "UniRef:UniRef90_P75058" ], "nt": "GTGACAGTACTAGTAGCCTTACTGGTTGCCTTTCCCTTTTGTTACTTTTTATCACAAAGTAAAAACAAGGTATTCCGCAGTTTTGTGATTGCCTTAGCTACTGCTCCCATATGGAGTAGTTTTCTCATTAAGTTAATTGGGTTAAAAACACTGTTGGACTTAGTTTTGGGGTTAGCGTTGAACCGGGTGGGTGATAATAACCTCACCTTTGGTAGTGGTTATACCTTGATTGGGATGATCTACCTGTTCACCCCCTTCATGTTTTTACCACTTTACAACAACTTTTGTATCTTACCGAAAAACTTAATCTTAGCTAGTCAAGATTTGGGTTACAACTGGATTACGAGTTTTATTAAGGTAGTTATTCCCTTTTCTAAAACTGCCATTCTGTCAGGGATTGCGTTAACTTTCTTTCCGTCTTTAACCTCGGTAGCGATTGCCCAGTTCTTGGACAACTCGAATCAAAATAACACCTTAGGTAACTATGTCTTTACTTTAGGTAACAATGGTTATGACAGTGCGATTGAGCGCGGCCGTGCTTCTGGTGCCATTATTATTGCGGCTTTAATTACCTTTGCCTTTTACTTTGTGGTGATCTTTGCTCCAAGAATAGTGCGCTTAATCCAGACTAAGTGTTTGAAATACAGGAGGGTTAATGTTTAA", "aa": "MTVLVALLVAFPFCYFLSQSKNKVFRSFVIALATAPIWSSFLIKLIGLKTLLDLVLGLALNRVGDNNLTFGSGYTLIGMIYLFTPFMFLPLYNNFCILPKNLILASQDLGYNWITSFIKVVIPFSKTAILSGIALTFFPSLTSVAIAQFLDNSNQNNTLGNYVFTLGNNGYDSAIERGRASGAIIIAALITFAFYFVVIFAPRIVRLIQTKCLKYRRVNV", "aa_hexdigest": "74deb28a5e8186ae46d2c289a5eb4c4c", "start_type": "GTG", "rbs_motif": null, "genes": [ "potB" ], "truncated": "5-prime", "pseudogene": { "start": 72086, "stop": 72946, "observations": { "mutation": [ 72260, 72272 ], "stop": [ 72260, 72272 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75058", "query_cov": 0.9590909090909091, "subject_cov": 0.7377622377622378, "identity": 1.0, "score": 404.0, "evalue": 5.11e-141, "gene_start": 10, "gene_end": 220, "reference_start": 76, "reference_end": 286, "reference_sequence": "MKLSKKYLLAVPFFVLMVIFFVVPMAWIIVSGLQNENGASITEKYQPLVGGYSFFQSFWTSLWTATVTVLVALLVAFPFCYFLSQSKNKVFRSFVIALATAPIWSSFLIKLIGLKTLLDLVLGLALNRVGDNNLTFGSGYTLIGMIYLFTPFMFLPLYNNFCILPKNLILASQDLGYNWITSFIKVVIPFSKTAILSGIALTFFPSLTSVAIAQFLDNSNQNNTLGNYVFTLGNNGYDSAIERGRASGAIIIAALITFAFYFVVIFAPRIVRLIQTKCLKYRRVNV" }, "paralog": false, "identity": 0.9370629370629371, "score": 520.0, "evalue": 6.89e-189, "description": "Internal stop codon at 72260, 72272. Nonsense mutation around 72260, 72272.", "psc": { "uniref90_id": "UniRef90_P75058", "gene": "potB", "product": "Spermidine/putrescine transport system permease protein PotB homolog", "uniref50_id": "UniRef50_P47289", "cog_id": "COG1176", "cog_category": "E", "go_ids": [ "GO:0005886", "GO:0055085" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75058", "UniRef:UniRef50_P47289", "COG:COG1176", "COG:E", "GO:0005886", "GO:0055085" ] }, "pscc": { "uniref50_id": "UniRef50_P47289", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47289" ], "product": "Spermidine/putrescine transport system permease protein PotB homolog" } }, "id": "APKECPOBEN_168", "locus": "GCA002090215_00127" }, { "type": "cds", "sequence": "contig_1", "start": 73007, "stop": 73216, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAATCGGGATCGAGACAATGACCGTAATTAAGCCAATAATGACAGAGTTGAGTAAAGGGATGGCAAAGTCCGCTTCCCCTAATCTTAGATATGCTGACTTGGCATCCGGGTTAGGGTTTAAAACATTACCAAAGTCTAAGACAATGTTACCCCTAGTCGAAGAACCGTTAAAACTAACTAAGACAACTATTATTAAAGGGAGGTAG", "aa": "MAIGIETMTVIKPIMTELSKGMAKSASPNLRYADLASGLGFKTLPKSKTMLPLVEEPLKLTKTTIIKGR", "aa_hexdigest": "2b3c3c00e5a33e4d820a425e9bb38ff8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7461.976099999998, "isoelectric_point": 9.996741676330569 }, "id": "APKECPOBEN_169", "locus": "GCA002090215_00128" }, { "type": "cds", "sequence": "contig_1", "start": 73218, "stop": 73673, "strand": "+", "frame": 3, "gene": "potC", "product": "Spermidine/putrescine transport system permease PotC-like protein", "db_xrefs": [ "COG:COG1177", "COG:E", "GO:0005886", "GO:0055085", "SO:0001217", "UniRef:UniRef50_P47290", "UniRef:UniRef90_P75057" ], "nt": "ATGACTGCCTTTGCTTTATTGCGATCCAGGCAATGGCTCAATAAAACAGTCTTTGGGATTGCTAACTTTTCTCTAGCAACTCCCGATATTATTACCGGGATATCGCTAGTGTTGTTATTTGCCAACACCTGGTTGAGCTTTAACCAACAGTTAGGTTTCTTTACTATTATTAGTTCCCACATCTCCTTTTCGGTGCCTTATGCCTTGGTGTTAATTTACCCAAAGATGCAAAAGTTAAACCGTAACTTAATCCTAGCGAGTCAGGATTTGGGTTACTCACCCATTGCTACCTTCTTTCACATTACCTTACCTTACTTACTACCGAGTATCTTGTCAGCCATTCTAGTAGTGTTTGCCACGAGCTTTGATGACTATGTGATTACTTCCTTAGTTCAAGGTTCAGTTAAAACAGTCGCCAGTGAGCTCTATTCCTTTAGAAAGGGGATTAAGGCCTGA", "aa": "MTAFALLRSRQWLNKTVFGIANFSLATPDIITGISLVLLFANTWLSFNQQLGFFTIISSHISFSVPYALVLIYPKMQKLNRNLILASQDLGYSPIATFFHITLPYLLPSILSAILVVFATSFDDYVITSLVQGSVKTVASELYSFRKGIKA", "aa_hexdigest": "f2806938ae3e849e0fff7f1ce6a673f7", "start_type": "ATG", "rbs_motif": null, "genes": [ "potC" ], "truncated": "both", "pseudogene": { "start": 72939, "stop": 73799, "observations": { "mutation": [ 72966, 73671, 73785 ], "stop": [ 72966, 73671, 73785 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75057", "query_cov": 1.0, "subject_cov": 0.527972027972028, "identity": 1.0, "score": 283.0, "evalue": 1.89e-94, "gene_start": 1, "gene_end": 151, "reference_start": 94, "reference_end": 244, "reference_sequence": "MFKMKSCKLWLRGSFFVIVLVLIYLPLIIVVLVSFNGSSTRGNIVLDFGNVLNPNPDAKSAYLRLGEADFAIPLLNSVIIGLITVIVSIPIAIMTAFALLRSRQWLNKTVFGIANFSLATPDIITGISLVLLFANTWLSFNQQLGFFTIISSHISFSVPYALVLIYPKMQKLNRNLILASQDLGYSPIATFFHITLPYLLPSILSAILVVFATSFDDYVITSLVQGSVKTVASELYSFRKGIKAWAIAFGTILILVSILAVLLVTLHKYLRFKHKEMLRVKQWKNS" }, "paralog": false, "identity": 0.9895104895104895, "score": 535.0, "evalue": 5.59e-196, "description": "Internal stop codon at 72966, 73671, 73785. Nonsense mutation around 72966, 73671, 73785.", "psc": { "uniref90_id": "UniRef90_P75057", "gene": "potC", "product": "Spermidine/putrescine transport system permease protein PotC homolog", "uniref50_id": "UniRef50_P47290", "cog_id": "COG1177", "cog_category": "E", "go_ids": [ "GO:0005886", "GO:0055085" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75057", "UniRef:UniRef50_P47290", "COG:COG1177", "COG:E", "GO:0005886", "GO:0055085" ] }, "pscc": { "uniref50_id": "UniRef50_P47290", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47290" ], "product": "Spermidine/putrescine transport system permease protein PotC homolog" } }, "id": "APKECPOBEN_170", "locus": "GCA002090215_00129" }, { "type": "cds", "sequence": "contig_1", "start": 73784, "stop": 74068, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAACAGTTAAAGTATTGGTTTAGTCTAATGGGACTAACCATGTCAGTTGGGTTAACTGCTTGTAGTTCCAACCAGTTTGTGTTTGCTAACTTTGAGTCGTATGTGTCGCCCTTACTTTTAGAAAGGGCAGAAGCCAAGCAACCAATGACCTTTCTTACTTATCCTACTAACGAAAAGTTAATTAACGGATTTGCTAACAATACCTATACGGTAGCAGTGGCCTCGAGTTATGCTGTGAGTGAACTGCAGCAACAAGGCCACTTACTACCAATTGATTGA", "aa": "MKKQLKYWFSLMGLTMSVGLTACSSNQFVFANFESYVSPLLLERAEAKQPMTFLTYPTNEKLINGFANNTYTVAVASSYAVSELQQQGHLLPID", "aa_hexdigest": "6dc6f5b0027591970040725680a8f495", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10535.000199999991, "isoelectric_point": 6.545373725891113 }, "id": "APKECPOBEN_171", "locus": "GCA002090215_00130" }, { "type": "cds", "sequence": "contig_1", "start": 74378, "stop": 75238, "strand": "+", "frame": 2, "gene": "potD", "product": "putative lipo MG045-like protein", "db_xrefs": [ "COG:COG0687", "COG:E", "GO:0005886", "SO:0001217", "UniRef:UniRef50_P75056", "UniRef:UniRef90_P75056" ], "nt": "GTGCACCATGAGGTTAAGAATACCACGGTAGATGTTAATCCTACAGGTAGCACCTTAAACTACTTTGGCAACGTTTACGAAAGTTTTGCAAATTTAGGTCTAAAACGTGATAACCTCAACACGCTCTTTGTCAATTCGGACTCCAACATTATTATTAATGAATTAGCTAATGGCAGAAGACAAGGTGGCATTGTTTACAATGGTGATGCCGTATATGCCGCTTTGGGCGGTGATCTCCGCGATGAAATTAATGAGAATAACTTGCCCAACGGCGATAACTTCCACATTGTGCAACCGAAACACTCGCCTGTGGCCCTAGACTTTCTCATTATTAACCAGCAACAAACTCACTTTAGAGATGCTGCACACCAGTTAATTTACCAGTTGGCCTTAGAAGGCGCTGATCAAACAGCAGAAGAATTATTAAAAACTGATGAAGAGAAAGGTACCAGTGATGAGGACTATTACACTTATGGTGCAATGCAGAATTTTAGTTACGTTAACTATGTTTCGCCTTTAAAGAATATTTCTGATGAAACCACTGGGATTGTGTTTAAGGAAAACAAACAAGCTGATACTAAGCAAGTTGTCAAACAACAAAGTCAATCAGAACAACAATCTGAATCAGCAGAAAAGGAAGAAACGGAACAAGATGATTTCTACACTGCTACTCTCAAAAGTTTGTTGAAAGCGGACAGTTTAGATGACAAAGCTAAGAAGTTAGTAGACACAATTAAAAAGACTTACAAGATCAAAAAAGCCGACAATATTAATTGGGCTAACTTAATCGAGAAACCAATTACCCCATTACAAAGGAGTAATTTAACATTATCGTGGCTTGACTTTAAGCAACGCTTTTGA", "aa": "MHHEVKNTTVDVNPTGSTLNYFGNVYESFANLGLKRDNLNTLFVNSDSNIIINELANGRRQGGIVYNGDAVYAALGGDLRDEINENNLPNGDNFHIVQPKHSPVALDFLIINQQQTHFRDAAHQLIYQLALEGADQTAEELLKTDEEKGTSDEDYYTYGAMQNFSYVNYVSPLKNISDETTGIVFKENKQADTKQVVKQQSQSEQQSESAEKEETEQDDFYTATLKSLLKADSLDDKAKKLVDTIKKTYKIKKADNINWANLIEKPITPLQRSNLTLSWLDFKQRF", "aa_hexdigest": "afa7e0c61d971dadb4325a3fccb343cc", "start_type": "GTG", "rbs_motif": null, "genes": [ "potD" ], "truncated": "5-prime", "pseudogene": { "start": 74078, "stop": 75238, "observations": { "mutation": [ 74273 ], "stop": [ 74273 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75056", "query_cov": 1.0, "subject_cov": 0.5896907216494846, "identity": 0.997, "score": 536.0, "evalue": 3.64e-189, "gene_start": 1, "gene_end": 286, "reference_start": 199, "reference_end": 484, "reference_sequence": "MKKQLKYWFSLMGLTMSVGLTACSSNQFVFANFESYVSPLLLERAEAKQPMTFLTYPTNEKLINGFANNTYTVAVASSYAVSELQQQGHLLPIDWAKFNLKKTQNGSNQATIQNKEDAKELFTKEIGDISGELLNWGVPYFLQDLVFVYRGEKIQELEGQDVTWSTIIKAIVNHKDRFNNNRLALIDDARTIFSLANVVHHEVKNTTVDVNPTGSTLNYFGNVYESFANLGLKRDNLNTLFVNSDSNIIINELANGRRQGGIVYNGDAVYAALGGDLRDEINENNLPNGDNFHIVQPKHSPVALDFLIINQQQTHFRDAAHQLIYQLALEGADQTAEELLKTDEEKGTSDEDYYTYGAMQNFSYVNYVSPLKNISDETTGIVFKENKQADTKQVVKQQSQSEQQSESAEKEETEQDDFYTATLKSLLKADSLDDKAKKLVDTIKKTYKIKKADNINWANLIEKPITPLQRSNLTLSWLDFKQRFW" }, "paralog": false, "identity": 0.9974093264248705, "score": 753.0, "evalue": 1.16e-276, "description": "Internal stop codon at 74273. Nonsense mutation around 74273.", "psc": { "uniref90_id": "UniRef90_P75056", "gene": "PotD", "product": "Uncharacterized lipoprotein MG045 homolog", "uniref50_id": "UniRef50_P75056", "cog_id": "COG0687", "cog_category": "E", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75056", "UniRef:UniRef50_P75056", "COG:COG0687", "COG:E", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75056", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75056" ], "product": "Uncharacterized lipoprotein MG045 homolog" } }, "id": "APKECPOBEN_172", "locus": "GCA002090215_00131" }, { "type": "cds", "sequence": "contig_1", "start": 75241, "stop": 75855, "strand": "+", "frame": 1, "gene": "tsaD", "product": "tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD", "db_xrefs": [ "COG:COG0533", "COG:J", "EC:2.3.1.234", "GO:0002949", "GO:0005506", "GO:0005737", "GO:0061711", "SO:0001217", "UniRef:UniRef50_P75055", "UniRef:UniRef90_P75055" ], "nt": "ATGGAACAACCTTTGTGTATCTTGGGAATTGAAACCACTTGTGATGACACTAGCATAGGGGTAATCACTGAGAGTAAGGTACAAGCACACATTGTTTTATCATCCGCCAAATTACACGCCCAAACTGGTGGCGTAGTGCCCGAAGTTGCAGCTAGGAGTCACGAACAAAACTTGCTTAAAGCACTGCAACAATCAGGGGTTGTTTTAGAACAAATTACTCACATTGCTTATGCCGCTAATCCGGGATTACCCGGTTGTCTGCATGTGGGAGCTACCTTTGCGCGCAGTTTAAGCTTCTTATTGGACAAACCCTTATTGCCCATCAACCACCTTTATGCCCACATCTTCTCGGCCTTAATTGACCAAGATATTAATCAGTTAAAACTCCCCGCTTTAGGCTTAGTGGTGTCCGGGGGGCACACTGCCATTTACCTAATTAAGTCCTTGTTTGACTTGGAGTTAATTGCTGAGACTAGCGATGATGCGATTGGCGAAGTTTATGATAAGGTGGGCCGCGCTATGGGTTTTCCTTATCCAGCTGGACCCCAGTTAGATAGTTTGTTTCAACCGGAGTTAGTTAAGTCACATTATTTCTTTCGTCCTTCCACGAAGTGA", "aa": "MEQPLCILGIETTCDDTSIGVITESKVQAHIVLSSAKLHAQTGGVVPEVAARSHEQNLLKALQQSGVVLEQITHIAYAANPGLPGCLHVGATFARSLSFLLDKPLLPINHLYAHIFSALIDQDINQLKLPALGLVVSGGHTAIYLIKSLFDLELIAETSDDAIGEVYDKVGRAMGFPYPAGPQLDSLFQPELVKSHYFFRPSTK", "aa_hexdigest": "4be1fae36d08950b16846e51b5ffc889", "start_type": "ATG", "rbs_motif": null, "genes": [ "tsaD" ], "truncated": "3-prime", "pseudogene": { "start": 75241, "stop": 76158, "observations": { "mutation": [ 75853 ], "stop": [ 75853 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75055", "query_cov": 1.0, "subject_cov": 0.6394984326018809, "identity": 1.0, "score": 407.0, "evalue": 3.64e-142, "gene_start": 1, "gene_end": 204, "reference_start": 1, "reference_end": 204, "reference_sequence": "MEQPLCILGIETTCDDTSIGVITESKVQAHIVLSSAKLHAQTGGVVPEVAARSHEQNLLKALQQSGVVLEQITHIAYAANPGLPGCLHVGATFARSLSFLLDKPLLPINHLYAHIFSALIDQDINQLKLPALGLVVSGGHTAIYLIKSLFDLELIAETSDDAIGEVYDKVGRAMGFPYPAGPQLDSLFQPELVKSHYFFRPSTKWTKFSYSGLKSQCFTKIKQLRERKGFNPQTHDWNEFASNFQATIIDHYINHVKDAIQQHQPQMLLLGGGVSANKYLREQVTQLQLPYLIAPLKYTSDNGAMIGFYANLLINGKNN" }, "paralog": false, "identity": 0.9967213114754099, "score": 608.0, "evalue": 1.73e-223, "description": "Internal stop codon at 75853. Nonsense mutation around 75853.", "psc": { "uniref90_id": "UniRef90_P75055", "gene": "tsaD", "product": "tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD", "ec_ids": [ "2.3.1.234" ], "uniref50_id": "UniRef50_P75055", "cog_id": "COG0533", "cog_category": "J", "go_ids": [ "GO:0002949", "GO:0005506", "GO:0005737", "GO:0061711" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75055", "EC:2.3.1.234", "UniRef:UniRef50_P75055", "COG:COG0533", "COG:J", "GO:0002949", "GO:0005506", "GO:0005737", "GO:0061711" ] }, "pscc": { "uniref50_id": "UniRef50_P75055", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75055" ], "product": "tRNA N6-adenosine threonylcarbamoyltransferase" } }, "id": "APKECPOBEN_173", "locus": "GCA002090215_00132" }, { "type": "cds", "sequence": "contig_1", "start": 75877, "stop": 76200, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAGTCACAGTGTTTTACCAAGATTAAACAGTTAAGGGAAAGGAAAGGCTTTAATCCCCAAACCCACGATTGGAATGAGTTTGCTTCTAACTTCCAGGCCACCATTATTGATCACTACATCAATCATGTCAAGGATGCCATTCAGCAACACCAACCGCAAATGTTGCTGTTAGGTGGTGGGGTAAGTGCTAATAAATACTTAAGAGAACAGGTAACACAGTTACAGCTGCCTTACTTAATTGCGCCGCTCAAGTACACGAGCGATAACGGGGCGATGATTGGTTTTTATGCTAATCTTTTAATTAATGGCAAAAACAATTAA", "aa": "MKSQCFTKIKQLRERKGFNPQTHDWNEFASNFQATIIDHYINHVKDAIQQHQPQMLLLGGGVSANKYLREQVTQLQLPYLIAPLKYTSDNGAMIGFYANLLINGKNN", "aa_hexdigest": "3504ff01fa778645fb7059f8541d679e", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12279.903899999987, "isoelectric_point": 9.335940742492678 }, "id": "APKECPOBEN_174", "locus": "GCA002090215_00133" }, { "type": "cds", "sequence": "contig_1", "start": 76184, "stop": 76414, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00438.25" ], "nt": "ATGGCAAAAACAATTAAACATCCCCGATGGGGTAGATATGTAGCAGAAGCAGTTGGTCGTGGTCACCCCGATAAGATCTGTGACCAGATTGCGGACAGTATCTTAGATGAATGCATTAAGCAAAGTCCGACCAGTCACGTTGCTTGTGAGGTCTTTGCTTCCAAAAATCTGATTATGGTGGGCGGGGAAATCCTTACCACTGGTTATGTTGATGTGGTCCAAACCGGTTGA", "aa": "MAKTIKHPRWGRYVAEAVGRGHPDKICDQIADSILDECIKQSPTSHVACEVFASKNLIMVGGEILTTGYVDVVQTG", "aa_hexdigest": "5e3f238bd5d55ef4d35541e6737fd9b2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00438.25", "name": "S-AdoMet_synt_N", "length": 60, "aa_cov": 0.7894736842105263, "hmm_cov": 1.0, "evalue": 8.849824752085885e-25, "score": 82.66057586669922, "start": 14, "stop": 73 } ], "seq_stats": { "molecular_weight": 8256.410599999996, "isoelectric_point": 6.243501853942871 }, "id": "APKECPOBEN_175", "locus": "GCA002090215_00134" }, { "type": "cds", "sequence": "contig_1", "start": 76430, "stop": 77044, "strand": "+", "frame": 2, "gene": "metK", "product": "methionine adenosyltransferase", "db_xrefs": [ "COG:COG0192", "COG:H", "EC:2.5.1.6", "GO:0000287", "GO:0004478", "GO:0005524", "GO:0005737", "GO:0006556", "GO:0006730", "KEGG:K00789", "SO:0001217", "UniRef:UniRef50_P78003", "UniRef:UniRef90_P78003" ], "nt": "TTGGGTTACACCGAAAATGACTTTAGCTTTTTGAGTTGTATTAACTCCCAGTCCAGTGAAATTAACCAAGCTGTGCAAAGTAATGACGAAATAGGCGCGGGCGACCAGGGCATTACCGTTGGTTATGCTTGTAGTGAAACCGAGCAATTAATGCCCTTAGGGAGTATCGTAGCGCAAGCGCTGGTGCAACGCGCTGCTCGTATCATTGACCAATACCCCTTCATTAAACACGACATGAAGAGTCAGGTAGTGCTCAACTATACTGGTAACAAAGTTCAATGTGAATCAGTTTTAATGTCGGTACAACATACTCAGGACGTAAGTTTAGACCAACTGCGCCAAACAATTATCAACCAAGTGATCCTCCCCGTATTAACGGAGTACGGTTTAAATGATCCTAAAATCAAACACCTAGTCAATCCCGGGGGTTCGTTTGTCGTTGGGGGCCCGATGGCCGATACGGGGTTAACCGGACGCAAGATTATTGTGGACACCTATGGTCCGTACGCTAATCATGGTGGGGGGAGTTTTAGTGGTAAAGACCCTACCAAGGTCGACCGCACTGGAGCTTACTTTGCGCGTTTTATTGCTAAACACATTGTTAGTTTGGGTTGA", "aa": "MGYTENDFSFLSCINSQSSEINQAVQSNDEIGAGDQGITVGYACSETEQLMPLGSIVAQALVQRAARIIDQYPFIKHDMKSQVVLNYTGNKVQCESVLMSVQHTQDVSLDQLRQTIINQVILPVLTEYGLNDPKIKHLVNPGGSFVVGGPMADTGLTGRKIIVDTYGPYANHGGGSFSGKDPTKVDRTGAYFARFIAKHIVSLG", "aa_hexdigest": "546d2582b694afbac1d70544e267a248", "start_type": "TTG", "rbs_motif": null, "genes": [ "metK" ], "truncated": "both", "pseudogene": { "start": 76184, "stop": 77335, "observations": { "mutation": [ 76412, 77042, 77180, 77279 ], "stop": [ 76412, 77042, 77180, 77279 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P78003", "query_cov": 1.0, "subject_cov": 0.5326370757180157, "identity": 0.995, "score": 405.0, "evalue": 1.92e-140, "gene_start": 1, "gene_end": 204, "reference_start": 83, "reference_end": 286, "reference_sequence": "MAKTIKHPRWGRYVAEAVGRGHPDKICDQIADSILDECIKQSPTSHVACEVFASKNLIMVGGEILTTGYVDVVQTGWKVLNRLGYTENDFSFLSCINSQSSEINQAVQSNDEIGAGDQGITVGYACSETEQLMPLGSIVAQALVQRAARIIDQYPFIKHDMKSQVVLNYTGNKVQCESVLMSVQHTQDVSLDQLRQTIINQVILPVLTEYGLNDPKIKHLVNPGGSFVVGGPMADTGLTGRKIIVDTYGPYANHGGGSFSGKDPTKVDRTGAYFARFIAKHIVSLGWAEECEVSISWVFSQPLPQSIQVKCFNINKEFSEQLINQVISQYFNWSVAKIIAKLKLLDQVEYFRYAVYGHFGHQTAPWEQLSERDSLQCLIKNFQ" }, "paralog": false, "identity": 0.9895561357702349, "score": 755.0, "evalue": 8.45e-281, "description": "Internal stop codon at 76412, 77042, 77180, 77279. Nonsense mutation around 76412, 77042, 77180, 77279.", "psc": { "uniref90_id": "UniRef90_P78003", "gene": "metK", "product": "methionine adenosyltransferase", "ec_ids": [ "2.5.1.6" ], "uniref50_id": "UniRef50_P78003", "cog_id": "COG0192", "cog_category": "H", "kegg_orthology_id": "K00789", "go_ids": [ "GO:0000287", "GO:0004478", "GO:0005524", "GO:0005737", "GO:0006556", "GO:0006730" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P78003", "EC:2.5.1.6", "UniRef:UniRef50_P78003", "COG:COG0192", "COG:H", "KEGG:K00789", "GO:0000287", "GO:0004478", "GO:0005524", "GO:0005737", "GO:0006556", "GO:0006730" ] }, "pscc": { "uniref50_id": "UniRef50_P78003", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P78003" ], "product": "S-adenosylmethionine synthase" } }, "id": "APKECPOBEN_176", "locus": "GCA002090215_00135" }, { "type": "cds", "sequence": "contig_1", "start": 77311, "stop": 77559, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAN4XDC4", "UniRef:UniRef90_A0AAN4XDC4" ], "nt": "ATGTTTAATCAAAAATTTCCAATAAAAGCTCCTAAAGCACCATTTTTTAAAAAGAAAACACCACCAGTACCACCAAAGAATCCTGTGGTAACCAGCACTACCCCAGAACTCAAGAAAACCAAAATTAAAAAAGTAAAGGAAGCAGGGGATACCAATCTTTGGGATAAGATTAAAGCAAAGGTAAAAAAACACCCTTACCTGTTTGCTGTTTTAGTGTCCTTTATTCTTTTAATTGCACTTTTAGGCTGA", "aa": "MFNQKFPIKAPKAPFFKKKTPPVPPKNPVVTSTTPELKKTKIKKVKEAGDTNLWDKIKAKVKKHPYLFAVLVSFILLIALLG", "aa_hexdigest": "6bd778bf36dda1a40e9e959f53a815cd", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0AAN4XDC4", "query_cov": 1.0, "subject_cov": 0.8817204301075269, "identity": 1.0, "score": 155.0, "evalue": 5.23e-48, "valid": true, "uniref50_id": "UniRef50_A0AAN4XDC4" }, "pscc": { "uniref50_id": "UniRef50_A0AAN4XDC4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAN4XDC4" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9242.1835, "isoelectric_point": 10.335845375061034 }, "id": "APKECPOBEN_177", "locus": "GCA002090215_00136" }, { "type": "cds", "sequence": "contig_1", "start": 77623, "stop": 77841, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02978.25" ], "nt": "ATGACGCGTGAAGAACGCCGGCACCCCAAATTAATTAATCGTGACCCATCGCGAAAACAACGCATCATTAAGGGTTCGGGACGTAAAATGGATGAACTCAATAAGCTAATGAAGGAGTGGAGTAAACTGCAAACGAAAACAGCAGAAATGGGCCGGATGCTGAAAGGTGGTAAAAATCCCTTTAGTGGCTTTGGTGGACTTGGTGGCTTAGGTTTTTAA", "aa": "MTREERRHPKLINRDPSRKQRIIKGSGRKMDELNKLMKEWSKLQTKTAEMGRMLKGGKNPFSGFGGLGGLGF", "aa_hexdigest": "f4b5c3aba86d1e43626ba913840e938b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02978.25", "name": "SRP_SPB", "length": 45, "aa_cov": 0.625, "hmm_cov": 0.9555555555555556, "evalue": 1.129302526283557e-12, "score": 43.64171600341797, "start": 1, "stop": 45 } ], "seq_stats": { "molecular_weight": 8217.570200000002, "isoelectric_point": 11.053314018249512 }, "id": "APKECPOBEN_178", "locus": "GCA002090215_00137" }, { "type": "cds", "sequence": "contig_1", "start": 77860, "stop": 78582, "strand": "-", "frame": 1, "gene": "ffh", "product": "signal recognition particle protein", "db_xrefs": [ "COG:COG0541", "COG:U", "EC:3.6.5.4", "GO:0003924", "GO:0005048", "GO:0005525", "GO:0006614", "GO:0008312", "GO:0009306", "GO:0016887", "GO:0048500", "GO:0048501", "KEGG:K03106", "SO:0001217", "UniRef:UniRef50_P75054", "UniRef:UniRef90_P75054" ], "nt": "ATGTTAGTGGCACTAGACATCTACCGCCCCGCTGCGATTGACCAGTTAGCGACCCTAGCCGAACAAACCGAGTCTGTCTTCTTTGCCAAGGGTACCCAAGCCCCCGACCAAACCACCAAGGAGGCCGTTAAGACCTTTAAGGAAAGCGGTTGTCAAGCAATAATCTGTGACACGGCGGGACGGTTACAAACTAATCAGGAATTAATGGATGAGTTGGTAGCAATTAAAAACGAACTCCACCCCGATGAAATCCTAATGGTGGTCGATGGTTTAAGTGGCCAGGAAATTATTAATGTTGCCAAGGAGTTTCATAACCGTTTAAAACTCACCGGTTTTATTATTACCAAGCTTGACAGTGATGCCCGCGCTGGAGCGGCTTTGTCCTTAACGAGTTTGTTGGAAGTTCCGATTAAACTAATGGGGACATCAGAAAAACTCACAGGTCTAGAACAATTTCACCCAGAACGGATTGCCAGTCGGATCTTGGGTTTAGGGGATGTGATGACCTTAGTGGAAAAAGCGGAAGAAGTATTTGACAAACAGAGCTTAACCAAAACAGTTAGCAAGATGTTCTTGGGCAAGATGGACCTGGAAGATCTGTTGCTTTACATGGAACAGATGAACCAAATGGGTTCTGTTAGTAGTATTGTCAAGATGCTCCCGGGTAACTTAACGGTATCGGATGATCATGTAGAGAGCATTGAAGCCAAGGTTAAACTGTGA", "aa": "MLVALDIYRPAAIDQLATLAEQTESVFFAKGTQAPDQTTKEAVKTFKESGCQAIICDTAGRLQTNQELMDELVAIKNELHPDEILMVVDGLSGQEIINVAKEFHNRLKLTGFIITKLDSDARAGAALSLTSLLEVPIKLMGTSEKLTGLEQFHPERIASRILGLGDVMTLVEKAEEVFDKQSLTKTVSKMFLGKMDLEDLLLYMEQMNQMGSVSSIVKMLPGNLTVSDDHVESIEAKVKL", "aa_hexdigest": "4891d992f0905574c6de6c793e500a31", "start_type": "ATG", "rbs_motif": null, "genes": [ "ffh" ], "truncated": "both", "pseudogene": { "start": 77650, "stop": 78882, "observations": { "mutation": [ 77862, 78612, 78756 ], "stop": [ 77862, 78612, 78756 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75054", "query_cov": 1.0, "subject_cov": 0.5333333333333333, "identity": 1.0, "score": 454.0, "evalue": 4.04e-158, "gene_start": 1, "gene_end": 240, "reference_start": 132, "reference_end": 371, "reference_sequence": "MFKSMISSIVMRSMQKKINAQTISETDVQAVLKEIRIALLDADVNLLVVKNFIKAIREQTVGQTVEPGQDLQKWLLKVIKQELINILSQPNQEITSKRPLKVMVVGLQGSGKTTTCGKLAVWLKKQFQQKAMLVALDIYRPAAIDQLATLAEQTESVFFAKGTQAPDQTTKEAVKTFKESGCQAIICDTAGRLQTNQELMDELVAIKNELHPDEILMVVDGLSGQEIINVAKEFHNRLKLTGFIITKLDSDARAGAALSLTSLLEVPIKLMGTSEKLTGLEQFHPERIASRILGLGDVMTLVEKAEEVFDKQSLTKTVSKMFLGKMDLEDLLLYMEQMNQMGSVSSIVKMLPGNLTVSDDHVESIEAKVKLWKVLINSMTREERRHPKLINRDPSRKQRIIKGSGRKMDELNKLMKEWSKLQTKTAEMGRMLKGGKNPFSGFGGLGGLGF" }, "paralog": false, "identity": 0.9926829268292683, "score": 775.0, "evalue": 1.07e-286, "description": "Internal stop codon at 77862, 78612, 78756. Nonsense mutation around 77862, 78612, 78756.", "psc": { "uniref90_id": "UniRef90_P75054", "gene": "ffh", "product": "signal recognition particle protein", "ec_ids": [ "3.6.5.4" ], "uniref50_id": "UniRef50_P75054", "cog_id": "COG0541", "cog_category": "U", "kegg_orthology_id": "K03106", "go_ids": [ "GO:0003924", "GO:0005048", "GO:0005525", "GO:0006614", "GO:0008312", "GO:0009306", "GO:0016887", "GO:0048500", "GO:0048501" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75054", "EC:3.6.5.4", "UniRef:UniRef50_P75054", "COG:COG0541", "COG:U", "KEGG:K03106", "GO:0003924", "GO:0005048", "GO:0005525", "GO:0006614", "GO:0008312", "GO:0009306", "GO:0016887", "GO:0048500", "GO:0048501" ] }, "pscc": { "uniref50_id": "UniRef50_P75054", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75054" ], "product": "Signal recognition particle protein" } }, "id": "APKECPOBEN_179", "locus": "GCA002090215_00138" }, { "type": "cds", "sequence": "contig_1", "start": 78754, "stop": 78975, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02881.24" ], "nt": "ATGTTTAAGTCAATGATCTCGAGCATCGTTATGCGCTCGATGCAAAAAAAGATCAACGCGCAAACGATCAGTGAAACCGATGTCCAAGCCGTTTTAAAGGAAATTCGGATTGCTCTTTTAGACGCTGACGTGAACCTGTTGGTGGTCAAAAACTTCATTAAGGCGATTCGCGAACAAACCGTTGGTCAAACCGTAGAACCCGGTCAGGACTTACAGAAGTGA", "aa": "MFKSMISSIVMRSMQKKINAQTISETDVQAVLKEIRIALLDADVNLLVVKNFIKAIREQTVGQTVEPGQDLQK", "aa_hexdigest": "bbb26c70de1b627167fec426a9fd998a", "start_type": "ATG", "rbs_motif": "AATAA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02881.24", "name": "SRP54_N", "length": 60, "aa_cov": 0.7945205479452054, "hmm_cov": 1.0, "evalue": 1.042833209060525e-15, "score": 53.18986892700195, "start": 16, "stop": 73 } ], "seq_stats": { "molecular_weight": 8202.591699999997, "isoelectric_point": 9.222411918640137 }, "id": "APKECPOBEN_180", "locus": "GCA002090215_00139" }, { "type": "cds", "sequence": "contig_1", "start": 79031, "stop": 79747, "strand": "+", "frame": 2, "gene": "deoD", "product": "purine-nucleoside phosphorylase", "db_xrefs": [ "COG:COG0813", "COG:F", "EC:2.4.2.1", "GO:0004731", "GO:0005829", "GO:0006139", "GO:0006152", "KEGG:K03784", "RefSeq:WP_010874419.1", "SO:0001217", "UniParc:UPI00001291C7", "UniRef:UniRef100_P75053", "UniRef:UniRef50_P47295", "UniRef:UniRef90_P75053" ], "nt": "ATGACACCGCACATAAACGCAAAAAAAGATGACATTGCTAAGGTTGTTTTAATGCCCGGTGATCCCTTACGTGCTAAGTGGATTGCGGAACAATTTATGGAAAAGCCCCGGCTCGTCAACGAAGTGCGAGGCATGTTAGCTTTTACCGGTCAGTATAAGGGTAAAACCATTACCATTATGGGCCATGGCATGGGGATTCCCTCAATTGGGATTTATTCTTATGAACTGATGAAGTTCTATGAGGTTAATACCATTATCCGAATTGGTAGTTGCGGGGCGTTGCAAGGTTCCTTAAACCTCCAGGACCTCATTATTGCCGCTAAGGCTTGGAGTGAGTCGATTTACGCTAACGACATGGGTGTGGAAGTGCCAGCAGACAAGATCTTAATGGCCTCCCCGCAACTAGTAGAGTTAGCTAAAAAGACCGCTAACCAACTTCAACTGGCCTTCCATGAGGGCTTGGTGTTCTGTGAGGATGCCTTCCACCAAATCAGGAAAGACGTGTTAAAGCTAGCACAAGAAAAACACGCTTTAGCAGTGGAAATGGAAGCGCATGCTCTGTACGCTAATGCCATGCTGCTCAACAAGCAAGCGTTAACGATGCTCACGGTGTCCGATTCTTTGGTAACCCATGCTGCTCTACCAGCGGAACAACGCCAAGCCACCTTTAAGAACATGGCTATCTTATCCTTGGAAATGGCTAGCCAACTCGTTTAA", "aa": "MTPHINAKKDDIAKVVLMPGDPLRAKWIAEQFMEKPRLVNEVRGMLAFTGQYKGKTITIMGHGMGIPSIGIYSYELMKFYEVNTIIRIGSCGALQGSLNLQDLIIAAKAWSESIYANDMGVEVPADKILMASPQLVELAKKTANQLQLAFHEGLVFCEDAFHQIRKDVLKLAQEKHALAVEMEAHALYANAMLLNKQALTMLTVSDSLVTHAALPAEQRQATFKNMAILSLEMASQLV", "aa_hexdigest": "d1622c1f8fd56247632af74339038384", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001291C7", "ncbi_nrp_id": "WP_010874419.1", "uniref100_id": "UniRef100_P75053", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001291C7", "RefSeq:WP_010874419.1", "UniRef:UniRef100_P75053" ] }, "ips": { "uniref100_id": "UniRef100_P75053", "uniref90_id": "UniRef90_P75053" }, "psc": { "uniref90_id": "UniRef90_P75053", "gene": "deoD", "product": "purine-nucleoside phosphorylase", "ec_ids": [ "2.4.2.1" ], "uniref50_id": "UniRef50_P47295", "cog_id": "COG0813", "cog_category": "F", "kegg_orthology_id": "K03784", "go_ids": [ "GO:0004731", "GO:0005829", "GO:0006139", "GO:0006152" ] }, "pscc": { "uniref50_id": "UniRef50_P47295", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47295" ], "product": "Purine nucleoside phosphorylase DeoD-type" }, "genes": [ "deoD" ], "id": "APKECPOBEN_181", "locus": "GCA002090215_00140" }, { "type": "cds", "sequence": "contig_1", "start": 79751, "stop": 80425, "strand": "+", "frame": 2, "gene": "deoC", "product": "deoxyribose-phosphate aldolase", "db_xrefs": [ "COG:COG0274", "COG:F", "EC:4.1.2.4", "GO:0004139", "GO:0005737", "GO:0006018", "GO:0009264", "GO:0016052", "KEGG:K01619", "RefSeq:WP_010874420.1", "SO:0001217", "UniParc:UPI00001291AA", "UniRef:UniRef100_P09924", "UniRef:UniRef50_P09924", "UniRef:UniRef90_P09924" ], "nt": "ATGAAATTAGAATACAACCGCATTATTGACAGTACACTACTTAAAGCTGATACCTTACCCCACGAAATTGATGCTTTATGTGCTGATGCTCATAAGTACCAGTTTTACGCAGTTTGTGTCAATCCTAGCTATGTGCGGTATGCCAAAAATATCCTTAAGGGTACGGGGGTGAAACTGTGCACTGTCGTTGGTTTTCCCTTAGGACAAACAACACAACGCCAGAAGGTGTATGAAACTAAGATTGCCATTAAGGAAGGTGCGGATGAAATTGACATGGTAATGAACATTGCCGAGTTCAAAAAGCGCTGTGCCTGTGTCATTAGTGAAATAAGGGCAGTCAAAAAGGTTTGTGGTAAGCGAACGCTCAAAGTCATTATTGAAACGGCTTTGTTAAACCAGGACGAAATTCGTGATGCCGTTAATGTCTGTATTGATGGTAACGCTGACTTTGTCAAAACTTCTACCGGGTTTTCCATGCGTGGTGCATCATTGGAAGATATCACAATTATGCGCGAAGCTAGTGGTAATCTCATTAAGATCAAAGCATCGGGTGGGGTTCAAACAGCGCAGCAATTCCTTGACTTCTTTAATGCTGGAGTTAGTCGGATTGGCACTTCCAATGCGGTCAAGATTATGGAAGAATTGCACAAACTAGAAAGCCATGAACATCGTTAA", "aa": "MKLEYNRIIDSTLLKADTLPHEIDALCADAHKYQFYAVCVNPSYVRYAKNILKGTGVKLCTVVGFPLGQTTQRQKVYETKIAIKEGADEIDMVMNIAEFKKRCACVISEIRAVKKVCGKRTLKVIIETALLNQDEIRDAVNVCIDGNADFVKTSTGFSMRGASLEDITIMREASGNLIKIKASGGVQTAQQFLDFFNAGVSRIGTSNAVKIMEELHKLESHEHR", "aa_hexdigest": "b1a5656f05421d9203c65b191539935d", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001291AA", "ncbi_nrp_id": "WP_010874420.1", "uniref100_id": "UniRef100_P09924", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001291AA", "RefSeq:WP_010874420.1", "UniRef:UniRef100_P09924" ] }, "ips": { "uniref100_id": "UniRef100_P09924", "uniref90_id": "UniRef90_P09924" }, "psc": { "uniref90_id": "UniRef90_P09924", "gene": "deoC", "product": "deoxyribose-phosphate aldolase", "ec_ids": [ "4.1.2.4" ], "uniref50_id": "UniRef50_P09924", "cog_id": "COG0274", "cog_category": "F", "kegg_orthology_id": "K01619", "go_ids": [ "GO:0004139", "GO:0005737", "GO:0006018", "GO:0009264", "GO:0016052" ] }, "pscc": { "uniref50_id": "UniRef50_P09924", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P09924" ], "product": "Deoxyribose-phosphate aldolase" }, "genes": [ "deoC" ], "id": "APKECPOBEN_182", "locus": "GCA002090215_00141" }, { "type": "cds", "sequence": "contig_1", "start": 80412, "stop": 80549, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02885.22" ], "nt": "ATGAACATCGTTAACCTTATTAGTAAAAAGCAACGCGGCAAGGCGTTAACGGAAACAGAAATTAACTGGTTTGTCCATTCCGTTAATAACAAAAGCTTGGCCGATTATCAGGTCAGTGCCTTTTTAATGGCAGTGTGA", "aa": "MNIVNLISKKQRGKALTETEINWFVHSVNNKSLADYQVSAFLMAV", "aa_hexdigest": "2ba27ac4f98986486a333c854ad6f3ed", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02885.22", "name": "Glycos_trans_3N", "length": 41, "aa_cov": 0.9111111111111111, "hmm_cov": 1.0, "evalue": 3.4909723894005224e-12, "score": 41.433345794677734, "start": 5, "stop": 45 } ], "seq_stats": { "molecular_weight": 5109.8771000000015, "isoelectric_point": 9.398990821838382 }, "id": "APKECPOBEN_183", "locus": "GCA002090215_00142" }, { "type": "cds", "sequence": "contig_1", "start": 80559, "stop": 81200, "strand": "+", "frame": 3, "gene": null, "product": "Pyrimidine-nucleoside phosphorylase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A354QR12" ], "nt": "ATGAATTCCAAGGAGTTGTTTTGTCTCACCAAAGCGATGGTGAAATCAGGAGAGAGCCTCCACTTTAACCACCACAGCAAGTTAAGTGTTGACAAACACTCCACTGGTGGTATTGGTGACAAGGTTTCCATTGCTTTAATTCCAATCTTAACTGCCTTAGATTACAGTGTCGCAAAACTGTCGGGTCGTGGTTTGGGCTACACTGGTGGCACAATTGACAAATTGGAAGCAGTGGGAGTAAAAACAGATTTTACACCAACAGAGGCGCAGAACTTACTAGACCAAAATGACTGCTTTATTATTGGTCAAAGTGAGGGCATTGCTCCCGTAGACAAGGTGTTGTATGCACTACGCGATACTACGGCAACTGTGGACAGTCTGCCCTTAATTGCTTCCAGTGTCATGTCGAAGAAGTTGGCAATTAACAACGACTACATCTTCATTGACCTTAAGTACGGTAAGGGCGCTTTCTGTAAAACGAAGACGATGGCTAAAGAGCTAGCGCAGTACATGTACAGTATTGCCAAGCAGTTTAAGCGCAAACTCTACATTAAACTAAGTGACATGAACCAGGTTTTGGGTAAAACGATTGGTAATGCGTTAGAGGTCTTGGAAGTAGTGCACTTCTTAAAACGGAATTGA", "aa": "MNSKELFCLTKAMVKSGESLHFNHHSKLSVDKHSTGGIGDKVSIALIPILTALDYSVAKLSGRGLGYTGGTIDKLEAVGVKTDFTPTEAQNLLDQNDCFIIGQSEGIAPVDKVLYALRDTTATVDSLPLIASSVMSKKLAINNDYIFIDLKYGKGAFCKTKTMAKELAQYMYSIAKQFKRKLYIKLSDMNQVLGKTIGNALEVLEVVHFLKRN", "aa_hexdigest": "655979a94d3c0d66a21478ef55532664", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI000A66C754", "query_cov": 0.9295774647887324, "subject_cov": 0.8016194331983806, "identity": 0.5, "score": 185.0, "evalue": 2.75e-55, "valid": false, "product": "thymidine phosphorylase", "ec_ids": [ "2.4.2.4" ], "uniref50_id": "UniRef50_A0A354QR12", "go_ids": [ "GO:0006206" ] }, "pscc": { "uniref50_id": "UniRef50_A0A354QR12", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A354QR12" ], "product": "Pyrimidine-nucleoside phosphorylase" }, "genes": [], "id": "APKECPOBEN_184", "locus": "GCA002090215_00143" }, { "type": "cds", "sequence": "contig_1", "start": 81207, "stop": 81677, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF07831.19" ], "nt": "GTGGGTGCTGACTTTATTCAGTTGATGGAGCAAATAGTCACTGAAATCCTTATAGAAACAAAACGTGCACCAAACAAACGGGCAGCTGTAGCACTTTACCACGCTACATTAGAGGGCGAAAAACCATGGCAGCGTTTTTTAAAATTTATTGAGCTGCAAGGTAGTAGTTGGGAACGTTTTTTGGATTTAAAAGAGCTGTTTAATCCCCAGTATAAAGCGCCAGTGTTAGCATCTCAAAGTGGTACTTTGAGCTATACCAGTCCGGTGGATTTAGCCATGGTCTCGATTAGCTTAGGTGCTGGTCGAATGGTTAAAACAGATCTGATTGACCCAATGGCGGGGATTAAGTTAGTGAAACAAGCTAACGAAGTGGTAAAAGCTGGGGACACAGTCTTAGAACTTTACAGCTCCAAACCAATTACGCCTGCCCACATAGAAGCTGCGCAGCACACCATTATTATTAAGCAGTAA", "aa": "MGADFIQLMEQIVTEILIETKRAPNKRAAVALYHATLEGEKPWQRFLKFIELQGSSWERFLDLKELFNPQYKAPVLASQSGTLSYTSPVDLAMVSISLGAGRMVKTDLIDPMAGIKLVKQANEVVKAGDTVLELYSSKPITPAHIEAAQHTIIIKQ", "aa_hexdigest": "64dfdcc931ed04824c3d8c7149eee9e6", "start_type": "GTG", "rbs_motif": "AAAA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07831.19", "name": "PYNP_C", "length": 73, "aa_cov": 0.46153846153846156, "hmm_cov": 1.0, "evalue": 5.582736745497688e-22, "score": 72.73250579833984, "start": 83, "stop": 154 } ], "seq_stats": { "molecular_weight": 17308.964399999993, "isoelectric_point": 6.839117622375488 }, "id": "APKECPOBEN_185", "locus": "GCA002090215_00144" }, { "type": "cds", "sequence": "contig_1", "start": 81730, "stop": 82089, "strand": "+", "frame": 1, "gene": "cdd", "product": "cytidine deaminase", "db_xrefs": [ "COG:COG0295", "COG:F", "EC:3.5.4.5", "GO:0004126", "GO:0005737", "GO:0005829", "GO:0008270", "GO:0008655", "GO:0009972", "GO:0042802", "KEGG:K01489", "SO:0001217", "UniRef:UniRef50_P75051", "UniRef:UniRef90_P75051" ], "nt": "GTGGTCAATCATGCCTACACGCCTTTTTCAAAGTTTAAGGTAGCTTGTATGTTGGTGGCCAATAACCAGGCATTTTATGGAGTCAATATTGAAAACGCTTCTTACCCAGTAACCCTGTGTGCGGAACGTAGTGCGATTGCTAACATGGTCACTAGTATCGGCAAGGCCACAATTGATTATGTCTTTGTGTACTTTGACACTAAAACTCCAACCAATTCACCTTGTGGCATGTGTCGGCAAAACATCTTTGAGTTTGCCACTGATAAGACCCAGTTATTCTGCATTGAGAAGGACAAGAGCTTTAAACAATTTACTATTCCCGAAATTTTAAAGGGAGGTTTTCGATCATATGAACAGTAA", "aa": "MVNHAYTPFSKFKVACMLVANNQAFYGVNIENASYPVTLCAERSAIANMVTSIGKATIDYVFVYFDTKTPTNSPCGMCRQNIFEFATDKTQLFCIEKDKSFKQFTIPEILKGGFRSYEQ", "aa_hexdigest": "5c45effc65b270d0ef05793c0fa32ae9", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75051", "query_cov": 1.0, "subject_cov": 0.8947368421052632, "identity": 0.992, "score": 246.0, "evalue": 1.24e-82, "valid": true, "gene": "cdd", "product": "cytidine deaminase", "ec_ids": [ "3.5.4.5" ], "uniref50_id": "UniRef50_P75051", "cog_id": "COG0295", "cog_category": "F", "kegg_orthology_id": "K01489", "go_ids": [ "GO:0004126", "GO:0005737", "GO:0005829", "GO:0008270", "GO:0008655", "GO:0009972", "GO:0042802" ] }, "pscc": { "uniref50_id": "UniRef50_P75051", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75051" ], "product": "Cytidine deaminase" }, "genes": [ "cdd" ], "id": "APKECPOBEN_186", "locus": "GCA002090215_00145" }, { "type": "cds", "sequence": "contig_1", "start": 82079, "stop": 82924, "strand": "+", "frame": 2, "gene": "manB", "product": "Phosphomannomutase", "db_xrefs": [ "COG:COG1109", "COG:G", "EC:5.4.2.8", "GO:0000287", "GO:0004615", "GO:0005975", "GO:0006166", "GO:0008973", "SO:0001217", "UniRef:UniRef50_P47299", "UniRef:UniRef90_P75050" ], "nt": "ATGAACAGTAACGCATACTTGGAAGCTCAAAGGTGGCTAAGCCATCCGCGGGTAAAACCCAACTTAAAGGAGGTCATTACGGCTATGAGTGCTGAGGAAATCGAGCACTTCTTTAGCTTAAAGAAACCGAGTTTTGGCACTGCTGGGGTACGGGGCAAAATGGCACCGGGGTACCATGGGATGAATGTGTTTTCCTATGCTTACTTAACCCAGGGTTATGTCAACTACATCCAGTCGTTAAACCCAACTAAAAAACCATTACGCTTTTTAGTAGCCAGAGACACTAGAAAACACGGGGCATTGTTTAATGGCATTGTGTGTGATGTGATTACCTCTATGGGTCATGTGGTCTATATGTTTGATAACAATGAACCAACACCAACACCATTAGTATCTTATGTGATTAAGAAGTACCACTTTGATGGTGGGGTGAATGTCACTGCTAGTCATAATCCTAAAACCGATAATGGCTTTAAGATTTACGATGGTCATGGCGCGCAGTTACTAGACTTCCAAACTGATCAATTAATCGCAATGTTACCCCCAGTGGTAACAATGTTAGATTTCGAACCACGTGGTAACAACGAGCTGTTACACTTTTTGGATAACGAAGTGGTCTACAAAAACTACTTTGATGATCTTAAGGAAAGCCTAGTAGTGGACAACGACAGCTTTAAAAACCTCCCGGTGGTCTTTACCGGGTTACATGGTACTTCGGTGAAGTTGTTACCAAGGTTTTTAACCTATTTGGGTTACAGCAACATTATTAGTGTCCAACCACAAAACGTGTTTGACGCTAACTTTGCTAATGCTGACCACCTCAACCCCGAAAGTAAGGATACCTGA", "aa": "MNSNAYLEAQRWLSHPRVKPNLKEVITAMSAEEIEHFFSLKKPSFGTAGVRGKMAPGYHGMNVFSYAYLTQGYVNYIQSLNPTKKPLRFLVARDTRKHGALFNGIVCDVITSMGHVVYMFDNNEPTPTPLVSYVIKKYHFDGGVNVTASHNPKTDNGFKIYDGHGAQLLDFQTDQLIAMLPPVVTMLDFEPRGNNELLHFLDNEVVYKNYFDDLKESLVVDNDSFKNLPVVFTGLHGTSVKLLPRFLTYLGYSNIISVQPQNVFDANFANADHLNPESKDT", "aa_hexdigest": "4dd14b0aa519f837ae19bfa0fa1c675f", "start_type": "ATG", "rbs_motif": null, "genes": [ "manB" ], "truncated": "3-prime", "pseudogene": { "start": 82079, "stop": 83227, "observations": { "mutation": [ 82922, 83012 ], "stop": [ 82922, 83012 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75050", "query_cov": 1.0, "subject_cov": 0.5072202166064982, "identity": 1.0, "score": 576.0, "evalue": 1.02e-203, "gene_start": 1, "gene_end": 281, "reference_start": 1, "reference_end": 281, "reference_sequence": "MNSNAYLEAQRWLSHPRVKPNLKEVITAMSAEEIEHFFSLKKPSFGTAGVRGKMAPGYHGMNVFSYAYLTQGYVNYIQSLNPTKKPLRFLVARDTRKHGALFNGIVCDVITSMGHVVYMFDNNEPTPTPLVSYVIKKYHFDGGVNVTASHNPKTDNGFKIYDGHGAQLLDFQTDQLIAMLPPVVTMLDFEPRGNNELLHFLDNEVVYKNYFDDLKESLVVDNDSFKNLPVVFTGLHGTSVKLLPRFLTYLGYSNIISVQPQNVFDANFANADHLNPESKDTWELARQYASNTKAKLMMAIDPDADRFAIAEWNPQTQDWHYFSGNESGVMVAYYKLKHKQFKRQPYIVTTVVSTDLVDKIAKKYGAFVKRTNVGFKFIGQAVNHFSKDNELVVAFEEAIGMMASDGLNREKDSFQAAAIMLEIARYCHNKGISLLEFYRGEIFGEFGDYYNWTVPHTIHGVNWKEKMEQVLHQLTTATIKEVVGHKITKIKNYVDINLVEYVLENGNWIKFRISGTEPKLKLYFNLSNGYLAALKHEAKKMHEFLVRLLNLDKA" }, "paralog": false, "identity": 0.9947643979057592, "score": 774.0, "evalue": 5.18e-284, "description": "Internal stop codon at 82922, 83012. Nonsense mutation around 82922, 83012.", "psc": { "uniref90_id": "UniRef90_P75050", "gene": "manB", "product": "Phosphomannomutase", "ec_ids": [ "5.4.2.8" ], "uniref50_id": "UniRef50_P47299", "cog_id": "COG1109", "cog_category": "G", "go_ids": [ "GO:0000287", "GO:0004615", "GO:0008973", "GO:0005975", "GO:0006166" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75050", "EC:5.4.2.8", "UniRef:UniRef50_P47299", "COG:COG1109", "COG:G", "GO:0000287", "GO:0004615", "GO:0008973", "GO:0005975", "GO:0006166" ] }, "pscc": { "uniref50_id": "UniRef50_P47299", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47299" ], "product": "Phosphomannomutase" } }, "id": "APKECPOBEN_187", "locus": "GCA002090215_00146" }, { "type": "cds", "sequence": "contig_1", "start": 83066, "stop": 83434, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02880.22" ], "nt": "ATGGTGGCTTACTATAAGCTCAAACACAAGCAGTTTAAGCGTCAACCTTACATTGTAACGACGGTAGTCTCTACTGATCTAGTGGATAAGATTGCCAAGAAGTACGGGGCTTTTGTCAAGCGCACAAATGTCGGCTTTAAGTTTATTGGTCAAGCTGTTAATCACTTCTCCAAAGACAATGAACTAGTAGTGGCCTTTGAAGAAGCGATTGGTATGATGGCTAGTGATGGTTTGAACCGCGAAAAGGATTCCTTTCAAGCAGCGGCCATCATGCTTGAAATTGCGCGCTACTGTCATAATAAAGGAATTAGCTTACTAGAGTTCTACCGTGGTGAGATCTTCGGTGAATTTGGTGATTACTATAACTGA", "aa": "MVAYYKLKHKQFKRQPYIVTTVVSTDLVDKIAKKYGAFVKRTNVGFKFIGQAVNHFSKDNELVVAFEEAIGMMASDGLNREKDSFQAAAIMLEIARYCHNKGISLLEFYRGEIFGEFGDYYN", "aa_hexdigest": "6c1c9e655b61d554a2ffffce33e9e9c4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02880.22", "name": "PGM_PMM_III", "length": 102, "aa_cov": 0.8360655737704918, "hmm_cov": 0.9215686274509803, "evalue": 3.3632088340940916e-16, "score": 54.914920806884766, "start": 3, "stop": 104 } ], "seq_stats": { "molecular_weight": 13994.958499999992, "isoelectric_point": 8.925535011291508 }, "id": "APKECPOBEN_188", "locus": "GCA002090215_00147" }, { "type": "cds", "sequence": "contig_1", "start": 83477, "stop": 83743, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00408.26" ], "nt": "ATGGAACAGGTATTACACCAGTTAACCACAGCTACCATTAAAGAGGTTGTGGGACACAAGATTACCAAGATTAAAAACTATGTCGACATTAACCTAGTCGAATATGTCTTGGAAAACGGTAACTGGATTAAGTTCCGCATTTCCGGTACAGAACCGAAGTTAAAGCTGTACTTTAACCTATCTAACGGTTACTTAGCAGCTTTAAAACACGAAGCGAAAAAGATGCACGAGTTCCTAGTGCGATTGCTCAACCTCGATAAAGCTTAG", "aa": "MEQVLHQLTTATIKEVVGHKITKIKNYVDINLVEYVLENGNWIKFRISGTEPKLKLYFNLSNGYLAALKHEAKKMHEFLVRLLNLDKA", "aa_hexdigest": "50f5ae0cde5c026f59fdc3449416d0b2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00408.26", "name": "PGM_PMM_IV", "length": 33, "aa_cov": 0.375, "hmm_cov": 0.9090909090909091, "evalue": 1.0440483784774064e-06, "score": 24.199241638183594, "start": 26, "stop": 58 } ], "seq_stats": { "molecular_weight": 10224.90009999999, "isoelectric_point": 9.398023796081542 }, "id": "APKECPOBEN_189", "locus": "GCA002090215_00148" }, { "type": "cds", "sequence": "contig_1", "start": 83711, "stop": 83965, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTAATTCCTTACTTGGGTAAATACGAAATTGTCGAAGGTGACACTAAGGTCGATCAACTGCAACAATTTAGTGTTGGTAACTTAGTGGAAGTCCACTTAACTGGTGCGATTCACATTCAAGGTCAAATTAAGGCTCTATACCAAGGAACAATTAACAAAGCGGTAGTGGAAGTCGAATTAACCACCAAAACGCAGTTAATTAACTTGCCTTTAGAAAACCTAAGCTTTATCGAGGTTGAGCAATCGCACTAG", "aa": "MLIPYLGKYEIVEGDTKVDQLQQFSVGNLVEVHLTGAIHIQGQIKALYQGTINKAVVEVELTTKTQLINLPLENLSFIEVEQSH", "aa_hexdigest": "27954ce4ce3ea4788752e13f3c5fb45b", "start_type": "GTG", "rbs_motif": "AACAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9389.689299999996, "isoelectric_point": 4.931999015808104 }, "id": "APKECPOBEN_190", "locus": "GCA002090215_00149" }, { "type": "cds", "sequence": "contig_1", "start": 84017, "stop": 84244, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTAAGGGTATTTCGGTCAATCCGAAAAAGCGGGTGTTAGTGACCAACACTGCCATTGTGGAAATGGATGCGGACAAGTTTGACGAAAAGTGTCAGTACATTCTCAAGCACAAACAAGTTAAACCCGAAGCAATTGCCCAAGTTAACGAATCTGGTGAAATCATTGATACTAACCAGTTTGCGCAAGCTCTAATGGAAGCTAATAAGGCCGAACAAGACGAATGA", "aa": "MLKGISVNPKKRVLVTNTAIVEMDADKFDEKCQYILKHKQVKPEAIAQVNESGEIIDTNQFAQALMEANKAEQDE", "aa_hexdigest": "e66cf13a4c2b6dfc63112170cf508120", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8447.5644, "isoelectric_point": 5.112008094787597 }, "id": "APKECPOBEN_191", "locus": "GCA002090215_00150" }, { "type": "cds", "sequence": "contig_1", "start": 84350, "stop": 84448, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTAGATGAAGACCGTTATTTAAAGGTGAAGATTAGCGAGAAGAATTTACTAGGTCGTTACATCTACATTAAGATGATCTACAGTGAAGATGCATGA", "aa": "MLDEDRYLKVKISEKNLLGRYIYIKMIYSEDA", "aa_hexdigest": "b3662008d42eb2cf2cc38dec11d7d1b6", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3911.5431000000017, "isoelectric_point": 6.060196113586425 }, "id": "APKECPOBEN_192", "locus": "GCA002090215_00151" }, { "type": "cds", "sequence": "contig_1", "start": 84452, "stop": 84607, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCGAAACCTCAAAGCTAAGGTAAAAGCTTTGGGATTTGACAACGAAATCTTAGATGTAAGGGTTTTGAAAGAACGCGAAGTCATCGAAGAAGTCTTTTCCTTAAAATCCGGCAAGCTGCCCCGTTCATTAAAGAACACCGCTTTTACAAAGTGA", "aa": "MRNLKAKVKALGFDNEILDVRVLKEREVIEEVFSLKSGKLPRSLKNTAFTK", "aa_hexdigest": "ee1c3213417f222481e8cba1a563eafc", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5860.871099999999, "isoelectric_point": 9.991777610778808 }, "id": "APKECPOBEN_193", "locus": "GCA002090215_00152" }, { "type": "cds", "sequence": "contig_1", "start": 84681, "stop": 85058, "strand": "-", "frame": 2, "gene": "secE", "product": "preprotein translocase subunit SecE", "db_xrefs": [ "COG:COG0690", "COG:U", "GO:0005886", "GO:0008320", "GO:0009306", "GO:0015450", "GO:0016020", "GO:0043952", "RefSeq:WP_010874425.1", "SO:0001217", "UniParc:UPI00001391AC", "UniRef:UniRef100_P75048", "UniRef:UniRef50_P47301", "UniRef:UniRef90_P75048" ], "nt": "ATGGAAAAAAAGAAACTCCCTTTTGGTTTAAAGAACAAGGAAAAACTCACCGCCTACAACGATGAGAAGATCCACGAGTTACACCGTCAACTAAAGGCCAAAATAGAAGCCAAGAAGGCCAAAGAGAAACAAGACAGTAAAACTAAAGACACAGATAAAAAAGTGGACCAAACCCCAAAGGTTAAGGTGCCTTTTACCAAGAAGTTTTCGAACCTGTGGTTTGGGATTGACAAAGAGGTTAACAAGATTGTCTGGGTAACAAGCAAGAAATTAATTACGATCTTTTTGTTAATTGTTTTAGTTAGTGCCATTATGATAGGGATCTACTTTGGCATTAACCACCTCTTTATTGCGCTTGGAGTTTTTAAAGGTAAATAA", "aa": "MEKKKLPFGLKNKEKLTAYNDEKIHELHRQLKAKIEAKKAKEKQDSKTKDTDKKVDQTPKVKVPFTKKFSNLWFGIDKEVNKIVWVTSKKLITIFLLIVLVSAIMIGIYFGINHLFIALGVFKGK", "aa_hexdigest": "98528c5cc6aa9788f61bf33ebaabfa7a", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001391AC", "ncbi_nrp_id": "WP_010874425.1", "uniref100_id": "UniRef100_P75048", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001391AC", "RefSeq:WP_010874425.1", "UniRef:UniRef100_P75048" ] }, "ips": { "uniref100_id": "UniRef100_P75048", "uniref90_id": "UniRef90_P75048" }, "psc": { "uniref90_id": "UniRef90_P75048", "gene": "secE", "product": "preprotein translocase subunit SecE", "uniref50_id": "UniRef50_P47301", "cog_id": "COG0690", "cog_category": "U", "go_ids": [ "GO:0005886", "GO:0008320", "GO:0009306", "GO:0015450", "GO:0016020", "GO:0043952" ] }, "pscc": { "uniref50_id": "UniRef50_P47301", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47301" ], "product": "Uncharacterized protein MG055" }, "genes": [ "secE" ], "id": "APKECPOBEN_194", "locus": "GCA002090215_00153" }, { "type": "cds", "sequence": "contig_1", "start": 85307, "stop": 85609, "strand": "-", "frame": 3, "gene": null, "product": "MPN070 family protein", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P75047", "UniRef:UniRef90_P75047" ], "nt": "ATGAAAGTGATTAAGAAGCTCAATCTGCTCAATGAACTAACGATGCTGGTGTGCATCTTCTTCTTTGTGTGCACTATCTCCCTAATTGGCATTGGCATTATGTATGACCTCATTAACAGAACTTCATTGAGTGCACCACGTCGTGATCCGATTTTTCGGAACCTCAACACAGTCTTAATTGTTTTAGGGGTGTTAGAAATCCTTTTAATGGTGGCCCAATTAGTTATGTCCAATATGGCAGCCAACATTATTAACGAGGTTGCCGAAAACGTCGAGCAAAAGTTTGCTAAAGCCTTGAAATGA", "aa": "MKVIKKLNLLNELTMLVCIFFFVCTISLIGIGIMYDLINRTSLSAPRRDPIFRNLNTVLIVLGVLEILLMVAQLVMSNMAANIINEVAENVEQKFAKALK", "aa_hexdigest": "50d308f747f8078bac6dedf0b9c74f0e", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 85226, "stop": 85609, "observations": { "mutation": [ 85309 ], "stop": [ 85309 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75047", "query_cov": 1.0, "subject_cov": 0.7874015748031497, "identity": 1.0, "score": 178.0, "evalue": 4.56e-56, "gene_start": 1, "gene_end": 100, "reference_start": 1, "reference_end": 100, "reference_sequence": "MKVIKKLNLLNELTMLVCIFFFVCTISLIGIGIMYDLINRTSLSAPRRDPIFRNLNTVLIVLGVLEILLMVAQLVMSNMAANIINEVAENVEQKFAKALKWSRFLPFGLLQLYCYHKIKLVTQTDNI" }, "paralog": false, "identity": 0.9921259842519685, "score": 243.0, "evalue": 5.92e-84, "description": "Internal stop codon at 85309. Nonsense mutation around 85309.", "psc": { "uniref90_id": "UniRef90_P75047", "product": "MPN070 family protein", "uniref50_id": "UniRef50_P75047", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75047", "UniRef:UniRef50_P75047", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75047", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75047" ], "product": "Uncharacterized protein MG055.2 homolog" } }, "id": "APKECPOBEN_195", "locus": "GCA002090215_00154" }, { "type": "cds", "sequence": "contig_1", "start": 85596, "stop": 86426, "strand": "-", "frame": 2, "gene": "rsmI", "product": "16S rRNA (cytidine(1402)-2'-O)-methyltransferase", "db_xrefs": [ "COG:COG0313", "COG:J", "EC:2.1.1.198", "GO:0000451", "GO:0005737", "GO:0008649", "GO:0008757", "GO:0070677", "KEGG:K07056", "RefSeq:WP_010874428.1", "SO:0001217", "UniParc:UPI00001391B2", "UniRef:UniRef100_P75046", "UniRef:UniRef50_P47302", "UniRef:UniRef90_P75046" ], "nt": "ATGCCAGCTCTTAAAGTAATTGCCACCCCCATTGGCAACATTGAGGAAGTTAGTCCGCGAGTTAAAGCGGCTTTAACCAAGTGTGAAGTGCTCTTTTGTGAGGACACCAGAGTAACCAAAAAACTCTTGGGCTTACTCGGCATTGACTTTTCAAATAAACAGTTCATTATTAACAACGAGTTTAAGGAAAAGCAAAACTTAAGTAAGGTAGCTAACTTAATCCACCAGTATCACTGTGGTTTGGTCAGTGATGCGGGTTATCCCTCAGTATCCGATCCCGGGCACATATTGGTGGAGTATGTCAGAACGCAACTACCCCAAATCGCAATAGAAGTAATTAATGGTCCTAGTGCGCTGGTTTGTGGTTTGGTAACCAGTGGCTTCCCCGAAAGTCCACTACTGTTCTTGGGCTTCTTGGACCACAAACCCACTCAAGTAACCCAAACACTCAAACACTACCAAAACTTCCAAGGCACCATTGTTTTATTTGAAGCGGTACACCGTTTACAACAAACACTGGAAGTAATCCAAACGGTGTTTAGTAATACTGAAGTTTTTGTAGGGCGGGAACTGACCAAGCTCCACGAAAGCCACTACTGGTTTAATACAAGTGCACCGCTGCCAGACATTACTCTAAAGGGCGAGTTTGTGATTGTGATTAACAACCACCACACCCAACCGGTGGGGCAATATTCCGACCAACTCTTAAAACAAGAAATTACCCAATTAGTCCAAATGGGCGTTAAGGTTAAGGATGCTTGTCATTATTTAGCTAAACGATTGCAATTAAAATCAAATAAGCTCTACACCTTATTCCATGAAAGTGATTAA", "aa": "MPALKVIATPIGNIEEVSPRVKAALTKCEVLFCEDTRVTKKLLGLLGIDFSNKQFIINNEFKEKQNLSKVANLIHQYHCGLVSDAGYPSVSDPGHILVEYVRTQLPQIAIEVINGPSALVCGLVTSGFPESPLLFLGFLDHKPTQVTQTLKHYQNFQGTIVLFEAVHRLQQTLEVIQTVFSNTEVFVGRELTKLHESHYWFNTSAPLPDITLKGEFVIVINNHHTQPVGQYSDQLLKQEITQLVQMGVKVKDACHYLAKRLQLKSNKLYTLFHESD", "aa_hexdigest": "641248ff0c4485d65d6f1c9609b67a84", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001391B2", "ncbi_nrp_id": "WP_010874428.1", "uniref100_id": "UniRef100_P75046", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001391B2", "RefSeq:WP_010874428.1", "UniRef:UniRef100_P75046" ] }, "ips": { "uniref100_id": "UniRef100_P75046", "uniref90_id": "UniRef90_P75046" }, "psc": { "uniref90_id": "UniRef90_P75046", "gene": "rsmI", "product": "16S rRNA (cytidine(1402)-2'-O)-methyltransferase", "ec_ids": [ "2.1.1.198" ], "uniref50_id": "UniRef50_P47302", "cog_id": "COG0313", "cog_category": "J", "kegg_orthology_id": "K07056", "go_ids": [ "GO:0000451", "GO:0005737", "GO:0008649", "GO:0008757", "GO:0070677" ] }, "pscc": { "uniref50_id": "UniRef50_P47302", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47302" ], "product": "Ribosomal RNA small subunit methyltransferase I" }, "genes": [ "rsmI" ], "id": "APKECPOBEN_196", "locus": "GCA002090215_00155" }, { "type": "cds", "sequence": "contig_1", "start": 86408, "stop": 86944, "strand": "-", "frame": 3, "gene": "rnmV", "product": "ribonuclease M5", "db_xrefs": [ "COG:COG1658", "COG:J", "EC:3.1.26.8", "GO:0005737", "GO:0006364", "GO:0019843", "GO:0043822", "GO:0046872", "KEGG:K05985", "RefSeq:WP_010874429.1", "SO:0001217", "UniParc:UPI00001391B7", "UniRef:UniRef100_P75045", "UniRef:UniRef50_P75045", "UniRef:UniRef90_P75045" ], "nt": "ATGGATAAAAAAACACGTCTAAAACTAGATGGGGTGATAGTGTGTGAGGGCAAAACTGACCAAGCCCGCTTACAACAGCTGTTTGATGTTAGTGTTATTACCACGAACGGTTCAGCTTTAAACCAAAGAACCATTAACCTGATTAAGGCTGTAGCTAAAAAACAACCGGTGATCCTCTTCTTGGACCCCGATGTTGCTGGTCAGAAAATTCGCCGGCAGTTAGAACAACACTTAGATAAGTATGAAAGTTGTTTTATAGCTCGGAAAGACATGAAACCCAACAGCACTAAAATTGGGGTAGCGGAAGCAACTGATGCAGCTTTAATCCAAGCGTTACAACAACGTCAGGTGTTCACTAAAACAACCCAACCCACTTTAAGCTGGGAACAATATTTGGAATTAAACCTCAACAGTAAAAGTAAACGGTTAGCGTTGTGTAACAAACTACACTTAAGCTACTTTAACCACAAGCAGCTCTTTCGCAAACTTAATTTACTCCAACTTACCTTTGACCAAGTATGCCAGCTCTTAAAGTAA", "aa": "MDKKTRLKLDGVIVCEGKTDQARLQQLFDVSVITTNGSALNQRTINLIKAVAKKQPVILFLDPDVAGQKIRRQLEQHLDKYESCFIARKDMKPNSTKIGVAEATDAALIQALQQRQVFTKTTQPTLSWEQYLELNLNSKSKRLALCNKLHLSYFNHKQLFRKLNLLQLTFDQVCQLLK", "aa_hexdigest": "44a08099613c1fb03f33930446392a15", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001391B7", "ncbi_nrp_id": "WP_010874429.1", "uniref100_id": "UniRef100_P75045", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001391B7", "RefSeq:WP_010874429.1", "UniRef:UniRef100_P75045" ] }, "ips": { "uniref100_id": "UniRef100_P75045", "uniref90_id": "UniRef90_P75045" }, "psc": { "uniref90_id": "UniRef90_P75045", "gene": "rnmV", "product": "ribonuclease M5", "ec_ids": [ "3.1.26.8" ], "uniref50_id": "UniRef50_P75045", "cog_id": "COG1658", "cog_category": "J", "kegg_orthology_id": "K05985", "go_ids": [ "GO:0005737", "GO:0006364", "GO:0019843", "GO:0043822", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_P75045", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75045" ], "product": "Ribonuclease M5" }, "genes": [ "rnmV" ], "id": "APKECPOBEN_197", "locus": "GCA002090215_00156" }, { "type": "cds", "sequence": "contig_1", "start": 86923, "stop": 87909, "strand": "-", "frame": 1, "gene": "prs", "product": "Ribose-phosphate pyrophosphokinase", "db_xrefs": [ "COG:COG0462", "COG:F", "EC:2.7.6.1", "GO:0000287", "GO:0002189", "GO:0004749", "GO:0005524", "GO:0005737", "GO:0006015", "GO:0006164", "GO:0009156", "GO:0016301", "KEGG:K00948", "RefSeq:WP_015344868.1", "SO:0001217", "UniParc:UPI000012DFC1", "UniRef:UniRef100_P75044", "UniRef:UniRef50_P47304", "UniRef:UniRef90_P75044" ], "nt": "ATGGATCGCCATAACCACGTTGTTTTTAGCTTATCGAAAACCCACGACTTAGTTAGTCGTATTTGTCAGAAACTCAAAATGCCCATGGGGTTAATTACCCATAATGAGTTTGCTGATGGGGAGACCTACATCCGCTTTGAGGAGTCTGTGCGCAATAAGGATGTTTTTATCTTTCAGTCTACCTGTGCGCCAGTCAACGATTCCTTAATGGAATTGTTAATAGCAATTGATGCCTTAAAACGGGGTAGTGCAAAAAGTATTACCGCCATTTTGCCGTACTATGGTTATGCACGGCAAGATCGCAAAACAATGGGACGCGAACCGATAACTAGTAAATTAGTAGCGGATTTGTTAACCACGGCTGGGGTGAGCCGCGTAGCTTTAACCGACATCCACAGCGACCAAACCCAGGGTTTCTTTAATATACCCGTGGATACATTGCGAACCTACCACGTCTTTTTAACCCGCACTGTAGAGCTGTTAGGTAAAAAAGACCTTGTGGTGGTGTCCCCCGATTATGGCGGTGTAAAGCGGGCAAGGTTAATTGCAACATCTTTGGAACTACCGCTAGCGATTATTGACAAGCGTCGCCCAGCCCACAATGTAGCGGAATCAATTAATGTCTTGGGTGAAGTAGCGAACAAAAACTGTCTAATAGTGGACGACATGATTGACACCGGTGGTACGGTAATAGCAGCAGCTAAACTACTCCGGGAACACCATGCCAAAAAGGTCTGTGTCATGGCCACCCATGGTTTGTTTAACGGTGAAGCACCGCAACGCTTCCAAAAGGCCTTTAACGAAGGCTTAGTGGACTATTTGTTTGTCTCCAACTCCATTCCTCAAACTAAGTTTGACCAGTGTCCCCAGTTTCAGGTAATTGATCTAGCACCGTTATTTGAAGAGGTGTTATTGTGTTACGCTAATAACTCTTCCATTTCTGCCATTTATACGCGCCACATTGAATGGATAAAAAAACACGTCTAA", "aa": "MDRHNHVVFSLSKTHDLVSRICQKLKMPMGLITHNEFADGETYIRFEESVRNKDVFIFQSTCAPVNDSLMELLIAIDALKRGSAKSITAILPYYGYARQDRKTMGREPITSKLVADLLTTAGVSRVALTDIHSDQTQGFFNIPVDTLRTYHVFLTRTVELLGKKDLVVVSPDYGGVKRARLIATSLELPLAIIDKRRPAHNVAESINVLGEVANKNCLIVDDMIDTGGTVIAAAKLLREHHAKKVCVMATHGLFNGEAPQRFQKAFNEGLVDYLFVSNSIPQTKFDQCPQFQVIDLAPLFEEVLLCYANNSSISAIYTRHIEWIKKHV", "aa_hexdigest": "dd64599c9672f3800c7156cd35f3261a", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000012DFC1", "ncbi_nrp_id": "WP_015344868.1", "uniref100_id": "UniRef100_P75044", "db_xrefs": [ "SO:0001217", "UniParc:UPI000012DFC1", "RefSeq:WP_015344868.1", "UniRef:UniRef100_P75044" ] }, "ips": { "uniref100_id": "UniRef100_P75044", "uniref90_id": "UniRef90_P75044" }, "psc": { "uniref90_id": "UniRef90_P75044", "gene": "prs", "product": "Ribose-phosphate pyrophosphokinase", "ec_ids": [ "2.7.6.1" ], "uniref50_id": "UniRef50_P47304", "cog_id": "COG0462", "cog_category": "F", "kegg_orthology_id": "K00948", "go_ids": [ "GO:0005737", "GO:0002189", "GO:0005524", "GO:0016301", "GO:0000287", "GO:0004749", "GO:0006015", "GO:0006164", "GO:0009156" ] }, "pscc": { "uniref50_id": "UniRef50_P47304", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47304" ], "product": "Ribose-phosphate pyrophosphokinase" }, "genes": [ "prs" ], "id": "APKECPOBEN_198", "locus": "GCA002090215_00157" }, { "type": "cds", "sequence": "contig_1", "start": 87896, "stop": 88339, "strand": "-", "frame": 3, "gene": "smpB", "product": "SsrA-binding protein", "db_xrefs": [ "COG:COG0691", "COG:O", "GO:0003723", "GO:0005829", "GO:0006450", "GO:0070929", "KEGG:K03664", "RefSeq:WP_010874431.1", "SO:0001217", "UniParc:UPI0000136022", "UniRef:UniRef100_P75043", "UniRef:UniRef50_P75043", "UniRef:UniRef90_P75043" ], "nt": "ATGCGAGTACTTGTCAACAATCCCAGAGCGCAATATGACTATTACCTTTTAACGGGTTATTGTGCTGGCTTAGTCTTAAAAGGTAGTGAAGTCAAATCGCTAGCTTTAGGGCAAGGTAGCTTAAAGGAAGCCTATGTTTTTATTGACAAGCACGAGGTCTATATTAAAGATTTTAGCATTTCGCCCTATGCCTTTTCAGGCGAGTTCAACCACCCCTTCAAACGGGTGAAAAAGCTCCTTTTAAACCGGAATGAGATTAAACAAATTACGGCACGCCAAAAGCAAGAAGGACTTTCCATTATTCCACTTAAAGTGTTCTTTAAAAATGGCAAAATTAAAATGGAAATCTGGTTGGCCAAACCTAAGAAAAAATTTGATAAACGTGAAGCCATCAAAAGTAAAACGATCCAGCGCGAATTGCGCCAACAATATGGATCGCCATAA", "aa": "MRVLVNNPRAQYDYYLLTGYCAGLVLKGSEVKSLALGQGSLKEAYVFIDKHEVYIKDFSISPYAFSGEFNHPFKRVKKLLLNRNEIKQITARQKQEGLSIIPLKVFFKNGKIKMEIWLAKPKKKFDKREAIKSKTIQRELRQQYGSP", "aa_hexdigest": "9ab4b4e87d11e641a2c854b507837d00", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000136022", "ncbi_nrp_id": "WP_010874431.1", "uniref100_id": "UniRef100_P75043", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000136022", "RefSeq:WP_010874431.1", "UniRef:UniRef100_P75043" ] }, "ips": { "uniref100_id": "UniRef100_P75043", "uniref90_id": "UniRef90_P75043" }, "psc": { "uniref90_id": "UniRef90_P75043", "gene": "smpB", "product": "SsrA-binding protein", "uniref50_id": "UniRef50_P75043", "cog_id": "COG0691", "cog_category": "O", "kegg_orthology_id": "K03664", "go_ids": [ "GO:0003723", "GO:0005829", "GO:0006450", "GO:0070929" ] }, "pscc": { "uniref50_id": "UniRef50_P75043", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75043" ], "product": "SsrA-binding protein" }, "genes": [ "smpB" ], "id": "APKECPOBEN_199", "locus": "GCA002090215_00158" }, { "type": "cds", "sequence": "contig_1", "start": 88346, "stop": 88723, "strand": "+", "frame": 2, "gene": "wcaA", "product": "putative glycosyltransferase MG060-like protein", "db_xrefs": [ "COG:COG0463", "COG:M", "EC:2.4.-.-", "GO:0016757", "SO:0001217", "UniRef:UniRef50_P47306", "UniRef:UniRef90_P75042" ], "nt": "ATGAAAATCTCTGTTATTATCTCCACTTACAACTGTGGTGCTTTAATTGTGAAAGCACTATGTTCATTAGTGTCAAACCAAACACCAGCATGTGAGTTAGAAGTGCTTGTGATTGATGATGGATCCATTGATAATACTAGGCAAATTATCAAAAAATTTCAAGCAAAAGTTAGCTTTACTTTAAAGTACTTTTACAAAAAAAACGGAAATTGGGGTAGTGTAATTAACTATGTCAAGGAAAACCGTTTGGCCAACGGCGATTGGATTACTGTTTTAGACAGTGATGATACCTTAAAACCTAATACACTCAACAAGTTGGCTAATTTAGTGGAAAAAGCGGATTATGACTTAGTAGTTTTTGACTACACTAAGTGCTGA", "aa": "MKISVIISTYNCGALIVKALCSLVSNQTPACELEVLVIDDGSIDNTRQIIKKFQAKVSFTLKYFYKKNGNWGSVINYVKENRLANGDWITVLDSDDTLKPNTLNKLANLVEKADYDLVVFDYTKC", "aa_hexdigest": "f298052b3cb4ab81b483fdf9067e1fd2", "start_type": "ATG", "rbs_motif": null, "genes": [ "wcaA" ], "truncated": "3-prime", "pseudogene": { "start": 88346, "stop": 89026, "observations": { "mutation": [ 88721, 88763, 88994 ], "stop": [ 88721, 88763, 88994 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75042", "query_cov": 1.0, "subject_cov": 0.4180602006688963, "identity": 1.0, "score": 249.0, "evalue": 2.29e-81, "gene_start": 1, "gene_end": 125, "reference_start": 1, "reference_end": 125, "reference_sequence": "MKISVIISTYNCGALIVKALCSLVSNQTPACELEVLVIDDGSIDNTRQIIKKFQAKVSFTLKYFYKKNGNWGSVINYVKENRLANGDWITVLDSDDTLKPNTLNKLANLVEKADYDLVVFDYTKCWKKIKLKIHTYPTWWKNMTRELQKQTPFCIPLGKFLKRNLFYKLPKLKEKVSFQDALYTASSLKLAKKVRHVNQSGGNYHFKRAGNSMSIPWNIKRFSAELDICKDLIRLNAQEIALVHLLRQQFRVQLKEKQIQLAVTRDFNFSGFSWYTRCFLWMVYQTMLKRYFYLQTTKQ" }, "paralog": false, "identity": 0.9867256637168141, "score": 447.0, "evalue": 1.18e-161, "description": "Internal stop codon at 88721, 88763, 88994. Nonsense mutation around 88721, 88763, 88994.", "psc": { "uniref90_id": "UniRef90_P75042", "gene": "WcaA", "product": "Uncharacterized glycosyltransferase MG060 homolog", "ec_ids": [ "2.4.-.-" ], "uniref50_id": "UniRef50_P47306", "cog_id": "COG0463", "cog_category": "M", "go_ids": [ "GO:0016757" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75042", "EC:2.4.-.-", "UniRef:UniRef50_P47306", "COG:COG0463", "COG:M", "GO:0016757" ] }, "pscc": { "uniref50_id": "UniRef50_P47306", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47306" ], "product": "Uncharacterized glycosyltransferase MG060" } }, "id": "APKECPOBEN_200", "locus": "GCA002090215_00159" }, { "type": "cds", "sequence": "contig_1", "start": 88772, "stop": 88996, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACCCGGGAACTACAAAAGCAAACACCGTTTTGTATTCCTCTAGGTAAGTTTTTGAAACGCAACCTGTTTTACAAGTTGCCCAAGCTCAAAGAAAAGGTGAGTTTTCAGGATGCCTTGTATACGGCTAGTAGTTTAAAGCTCGCTAAAAAAGTGCGTCATGTTAATCAGTCTGGTGGCAACTACCACTTTAAGCGTGCTGGTAATTCAATGAGTATTCCCTGA", "aa": "MTRELQKQTPFCIPLGKFLKRNLFYKLPKLKEKVSFQDALYTASSLKLAKKVRHVNQSGGNYHFKRAGNSMSIP", "aa_hexdigest": "d2894568273e4512d45f3cb13c0b2d79", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8528.9778, "isoelectric_point": 10.389483070373537 }, "id": "APKECPOBEN_201", "locus": "GCA002090215_00160" }, { "type": "cds", "sequence": "contig_1", "start": 89021, "stop": 89245, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGACATTTGTAAAGACTTAATTCGTTTAAATGCTCAGGAAATTGCCCTAGTGCACTTATTGCGACAACAGTTTCGGGTGCAACTTAAAGAAAAGCAGATACAACTAGCGGTTACCCGTGACTTTAACTTTAGTGGGTTCTCTTGGTACACCCGCTGCTTTTTATGGATGGTATACCAAACGATGTTAAAGCGCTACTTTTACTTACAAACAACTAAACAATAA", "aa": "MDICKDLIRLNAQEIALVHLLRQQFRVQLKEKQIQLAVTRDFNFSGFSWYTRCFLWMVYQTMLKRYFYLQTTKQ", "aa_hexdigest": "43eac58589072e5fdffa7564562ad337", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9156.707199999993, "isoelectric_point": 9.755694389343262 }, "id": "APKECPOBEN_202", "locus": "GCA002090215_00161" }, { "type": "cds", "sequence": "contig_1", "start": 89351, "stop": 89809, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTCTTCATGAATGCCTTAATGGTGATTGTAATCGTATTGTTTGCAGCCACTCTAGGTGTGGGTACTGCTGTTGGCTTTGCCTTTGTGGCGATTGCAACCTTTATTGGTGGTTCGTTTGCTTGGAGTATGCAAAGTACCATTTTGATCCTCCCGCACGAATTCAAAGAGTACAAGCGTAGTGAAGTGTCCGTGTTGTTTGGTTACATCTGGGGCTTTGGTTATGTGATCTACACCGCCTTTGACATTACTAACTCAATGTTCTTAGAAGCACCGAAACTAGCAAACCCTGGAATGAAAGGAGTAAGCATCCTCCCAGGGGCAATTGCTGGTGTGGCGCTGTTTGCTGGTTTGTTGTTAGCAGCCATTGCGATTGTGGTAACACTGCCATCTTCGTATCTTAAGAATGGCGATGAACTGGTAAGTGAAATGACCAAAAAGTGGAAGTTAAACCAGTGA", "aa": "MFFMNALMVIVIVLFAATLGVGTAVGFAFVAIATFIGGSFAWSMQSTILILPHEFKEYKRSEVSVLFGYIWGFGYVIYTAFDITNSMFLEAPKLANPGMKGVSILPGAIAGVALFAGLLLAAIAIVVTLPSSYLKNGDELVSEMTKKWKLNQ", "aa_hexdigest": "4da9470b75af747b10a78319ffb11673", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16400.338599999985, "isoelectric_point": 7.947485160827637 }, "id": "APKECPOBEN_203", "locus": "GCA002090215_00162" }, { "type": "cds", "sequence": "contig_1", "start": 89819, "stop": 89998, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAGTCCAGCGAGTTCGTTTAATGTTAAAGATGGTGTTAAAACATTAAAACCACTAGAGGGTGCTGGTGGTTACTTTGCTGGTCTCCCAACCTTAGCAATCCTCTGGGTATTAGGTTATGGTATGGGTTACATGGTGTTTTCACCATTTAACAAAACGGTTTACGATCGCAAGCGTTGA", "aa": "MSPASSFNVKDGVKTLKPLEGAGGYFAGLPTLAILWVLGYGMGYMVFSPFNKTVYDRKR", "aa_hexdigest": "309e4e3c168cbb206e83d532e66bab84", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6448.490000000002, "isoelectric_point": 9.69728603363037 }, "id": "APKECPOBEN_204", "locus": "GCA002090215_00163" }, { "type": "cds", "sequence": "contig_1", "start": 90080, "stop": 90757, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGTCCTGAAGTTTGGTTACCGTTATGCGGTCATGATTATGATGGGGCTAATGTGTCTCTGTTTCCCCTTCTTAATTATTGGTGATCCATTAGGGGGAAATAACCAGTTAACTTTATTGCGACCACTTTCAAAGGAAGTAATGGGTAAACTTTCTAGTCTATCTTCGCAATTACATGAAGGTCAATTGTTGGGGCCTGTTATGGCAGAAGGCAAGACCATGTTAGCAGATGGTCAAACTATTGATTTAGTTCAAGGTTTAGATAAAAATTTAATTGGTACCAGTTCATCAATTGCTGGTTATGCGTTGTTTATTATTTTCCGTTCCACGATTGCTATTGGTGGTACAACTTTAGTAACTTACTCCCAACCGTTAATTGCTTCACTTTCAACGCAACGTCGTAAGTCAGTGTTATCCAACGCGAACCTCTGGGGCTTTAACAGTGGGATTGTAGTTGCTTTTGTACCGTTCTTATTCCAATCTGTTCAACAAGCTGGTACTAAGTACTGGGTCTTTATCCTAACTGCTTTAATCTTGATTGGTTTTGGCATTCTCTGTGTGTTTGCTTGGTTTGAAAAGCAAATGGATCCGTTTATGCCCCAAAAGCAAACCAAGGAACAAATGCAGTTGGGTAACCAACCAAGTGCTGGTGATATCTTAAAGCGAAAAGCGACCTGA", "aa": "MVLKFGYRYAVMIMMGLMCLCFPFLIIGDPLGGNNQLTLLRPLSKEVMGKLSSLSSQLHEGQLLGPVMAEGKTMLADGQTIDLVQGLDKNLIGTSSSIAGYALFIIFRSTIAIGGTTLVTYSQPLIASLSTQRRKSVLSNANLWGFNSGIVVAFVPFLFQSVQQAGTKYWVFILTALILIGFGILCVFAWFEKQMDPFMPQKQTKEQMQLGNQPSAGDILKRKAT", "aa_hexdigest": "99fdaee3a16aeb0639d4665cbff6b736", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 24641.918399999988, "isoelectric_point": 9.454304695129395 }, "id": "APKECPOBEN_205", "locus": "GCA002090215_00164" }, { "type": "cds", "sequence": "contig_1", "start": 91581, "stop": 92015, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGCCCCAAAAGCAATCAAAAACCAATCAAAGCTTGAGTGTGAGACCAAGTGCGTTATCAATTTTGAAACGCAAAACAACTTGGAAGCTGTTGGCAATAGCTGGTGTAGGTACAATTCTCTTAATTAACCCGTTAACCCAAACCTGGTTTAACAGCTTGTTAGCTATTTCAGGTGCTAAAAAAGCAATTATTCCAACTGCCCGTCCAATCTTATTAATTCTCTGGGTAATGGGTTACCTCTTGGGTTACTTCTTACTGTCACCGTTTAACAAAACAATTTATGACAAGAAGCGTTGGCTCCACTTTATCTTTACGGCCAACGCCGTGTTGGTGTTGTTAATAGTAATCTTTGCTGCAACGCTTGGTTTAAATACTGTGGTTGGCTTTACGTTTGTGGGTATCTTTACCTTTATTGCTGGTGGTTTTGGTTGA", "aa": "MLPQKQSKTNQSLSVRPSALSILKRKTTWKLLAIAGVGTILLINPLTQTWFNSLLAISGAKKAIIPTARPILLILWVMGYLLGYFLLSPFNKTIYDKKRWLHFIFTANAVLVLLIVIFAATLGLNTVVGFTFVGIFTFIAGGFG", "aa_hexdigest": "c9bc3d292f9563757b86074e006a3536", "start_type": "ATG", "rbs_motif": "AAAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15868.987999999968, "isoelectric_point": 10.94539394378662 }, "id": "APKECPOBEN_206", "locus": "GCA002090215_00165" }, { "type": "cds", "sequence": "contig_1", "start": 92175, "stop": 92606, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTGGTGAAGTTTGGTTACCGTCATGCAGTGATGATCATGATGGGACTCATGTGTGTTTGTTTTCCTTTCTTAATTATCGGTAGTCCGTTAGGTGGTCATAACGAGTTGACCTTGTTGCGACCGGCTTCAAGTGAAGTAATAAGTAAACTTACTAAAATTTCTTCCAGTTTACAACAAGGTCAACTGTTAGGTCCTGTTCAAGTGGGTAGTCAAACTATGTTGGCTGACGGCACTCCTGTTAGCTTAATTAAAGGAATTAACGGCAACGAAATTGGTACCAGTGCGTCAATGACTGGTTATGCGTTTTTCATTATTTTCCGTTCTACGATTGCTATTGGTGGTACGACCTTAATTGCTTATGCGCAACCAATTATTGCTTCCCTTTCTTCAAACCGCAAGAAATCGATTTTATCGAATGCTAACTTCTGA", "aa": "MVVKFGYRHAVMIMMGLMCVCFPFLIIGSPLGGHNELTLLRPASSEVISKLTKISSSLQQGQLLGPVQVGSQTMLADGTPVSLIKGINGNEIGTSASMTGYAFFIIFRSTIAIGGTTLIAYAQPIIASLSSNRKKSILSNANF", "aa_hexdigest": "c22c84bb559ba32bb83c1a8ea59c2d6a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15126.707199999984, "isoelectric_point": 9.757821846008298 }, "id": "APKECPOBEN_207", "locus": "GCA002090215_00166" }, { "type": "cds", "sequence": "contig_1", "start": 92652, "stop": 92810, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTGTTTGTGATTGAATGGTTTGTGATTGACTTCATTAGAGGTAGTGCGGCTTCCCTTTTAACTGAACAAACTAGTGCTGTTCCCCAGTACGGTGGTTGGTTCAGTTCTTTTTTTGTAGCGGACGCTGGTTTTATTCCAGGTCAAGCTACTAACTGA", "aa": "MLFVIEWFVIDFIRGSAASLLTEQTSAVPQYGGWFSSFFVADAGFIPGQATN", "aa_hexdigest": "f91f5412a1721c9bc8b97dfb63678239", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5691.3793, "isoelectric_point": 4.0500284194946286 }, "id": "APKECPOBEN_208", "locus": "GCA002090215_00167" }, { "type": "tRNA", "sequence": "contig_1", "start": 93050, "stop": 93138, "strand": "-", "gene": "trnS", "product": "tRNA-Ser(gct)", "amino_acid": "Ser", "anti_codon": "gct", "score": 76.9, "nt": "GGGAGCGTACTCAAGTGGTTAAGAGGACACCCTGCTAAGGTGTTAGATCGTTCTACGGTGCGTGGGTTCGAATCCCACCGCTTCCGCCA", "db_xrefs": [ "SO:0000269" ], "anti_codon_pos": [ 93103, 93105 ], "id": "APKECPOBEN_35", "locus": "GCA002090215_00168" }, { "type": "cds", "sequence": "contig_1", "start": 93369, "stop": 94436, "strand": "+", "frame": 3, "gene": "fruA", "product": "PTS system fructose-specific EIIABC component", "db_xrefs": [ "COG:COG1762", "COG:GT", "GO:0005351", "GO:0005886", "GO:0009401", "GO:0016301", "GO:0022877", "GO:0090563", "SO:0001217", "UniRef:UniRef50_P47308", "UniRef:UniRef90_P75039" ], "nt": "ATGTCAAAACTGGCATTGCCCCACATGCAAAGTGCTACCGTGTTAAAACCGTTTGTGGCGGTGTTTAAGGTCAACAATGTGGACTGGCAATCCTTAGACAACCAACCGGTTAAACTAATCTTTTTAATTGGCGTACCCAAAGATCAAGGCAACCTCCACTTGGAATTTATTTCGCAGTTTTCCAAGTTAATGTTGCAGGACGAGTTTGCCAACAAAGTGCCCAACATTCGCAGCTTTAACGGGTTGATCAATTTAATTGACAGTTTTCAACAAACTGCAGTTGCAAGTCAACCTGTTGTTAACGAAGCAGCAGCTCAGACTGAAGAGCCAAAGGACACAAACACTCAGTATGACTTTGTGGCGGTAACGGCGTGTCCCACCGGGATTGCTCACACCTTTATGGCCAAGGAAGCGCTGGAAAAGTTTGCCCGCGATCACAACCTCAAAGTTAAGGTCGAAACTCAAGGAACTGATGGGATTCAGAACCAGTTAACCGAGTCTGATTTAAACAATACCAAAGGCATTATTTTGGCCTGTGACCGCTTAATTGATTTAACGCGTTTTTACGGTCACGCAAATGTGGTGGAAGTGTCCACAACCAAAGCAATTAAAACACCACAAACAGTTTATGACCAAGTGGTAAAGAAGGAAGGCAAACTACTCGGTAATAAGTCCAGTGATAGTGCTAGTCAAACGGAACTCAAAGAAACCACTGAACAGCTCTCGTTTAAAGACTTTCACAAACGGATTTACCGCGCCATTTTAAGTGGGGTTTCTTACATGCTGCCATTTGTCGTGTTTGGTGGCATCTTAATTGCAATTGCCTTTTTAATTGACATTAATAATGCTGGCAATGCTGGTAAACAATTTGGCTCTAAAGATCCCATAGCTAACTGGTTTAAAACACTCGGTGGGTTGTCGTTTGGTTTAATTGTTCCCATTTTAAGTGCTTACATTGCCTTTGCTCTAGTCGGCCGGCAAGGGTTACTGCCCGGCTTTATTGTGGGGTTAATTTCAGCTGGTAAGTTTTTACTCAACATTGACATTGTTACTGGAAAAATTGATTGA", "aa": "MSKLALPHMQSATVLKPFVAVFKVNNVDWQSLDNQPVKLIFLIGVPKDQGNLHLEFISQFSKLMLQDEFANKVPNIRSFNGLINLIDSFQQTAVASQPVVNEAAAQTEEPKDTNTQYDFVAVTACPTGIAHTFMAKEALEKFARDHNLKVKVETQGTDGIQNQLTESDLNNTKGIILACDRLIDLTRFYGHANVVEVSTTKAIKTPQTVYDQVVKKEGKLLGNKSSDSASQTELKETTEQLSFKDFHKRIYRAILSGVSYMLPFVVFGGILIAIAFLIDINNAGNAGKQFGSKDPIANWFKTLGGLSFGLIVPILSAYIAFALVGRQGLLPGFIVGLISAGKFLLNIDIVTGKID", "aa_hexdigest": "0363d2a8b599c9665c25ea3afe23be4c", "start_type": "ATG", "rbs_motif": null, "genes": [ "fruA" ], "truncated": "both", "pseudogene": { "start": 93189, "stop": 94739, "observations": { "mutation": [ 93228, 94434, 94617 ], "stop": [ 93228, 94434, 94617 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75039", "query_cov": 1.0, "subject_cov": 0.5115273775216138, "identity": 1.0, "score": 688.0, "evalue": 1.56e-244, "gene_start": 1, "gene_end": 355, "reference_start": 61, "reference_end": 415, "reference_sequence": "MFKPLLSAELFFNWTAKDFKDKTSFLKQACRVLQDKNCIKEEQIALTALKEREAQITTGIMSKLALPHMQSATVLKPFVAVFKVNNVDWQSLDNQPVKLIFLIGVPKDQGNLHLEFISQFSKLMLQDEFANKVPNIRSFNGLINLIDSFQQTAVASQPVVNEAAAQTEEPKDTNTQYDFVAVTACPTGIAHTFMAKEALEKFARDHNLKVKVETQGTDGIQNQLTESDLNNTKGIILACDRLIDLTRFYGHANVVEVSTTKAIKTPQTVYDQVVKKEGKLLGNKSSDSASQTELKETTEQLSFKDFHKRIYRAILSGVSYMLPFVVFGGILIAIAFLIDINNAGNAGKQFGSKDPIANWFKTLGGLSFGLIVPILSAYIAFALVGRQGLLPGFIVGLISAGKFLLNIDIVTGKIDWATESKVSSGFFGAIFGGLLAAVLIIVQQRYIYRKLPQALQGIKNILFIPLLGTLVTAALFWVINIPLIYLNYGLSKFLQIMDKPYLAPLLGLVIGLMMCFDLGGPVNKAAYVFGVVSLESQNSGTVAMASAILSGMVPPLGIAIAATIRKQCFDKEELPAAYACYVMGLSFISEGAIPFVAKRPKIMLAANLIGGAVCGVLTGAFALTIRAPHGGVFVFALLKTNLEGIAGNTLQIGAGVGLALLALIVSSFISAGIIIGHNLLVVRKKTKQLVNTNA" }, "paralog": false, "identity": 0.9941860465116279, "score": 994.0, "evalue": 0.0, "description": "Internal stop codon at 93228, 94434, 94617. Nonsense mutation around 93228, 94434, 94617.", "psc": { "uniref90_id": "UniRef90_P75039", "gene": "fruA", "product": "PTS system fructose-specific EIIABC component", "uniref50_id": "UniRef50_P47308", "cog_id": "COG1762", "cog_category": "GT", "go_ids": [ "GO:0005886", "GO:0005351", "GO:0016301", "GO:0022877", "GO:0090563", "GO:0009401" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75039", "UniRef:UniRef50_P47308", "COG:COG1762", "COG:GT", "GO:0005886", "GO:0005351", "GO:0016301", "GO:0022877", "GO:0090563", "GO:0009401" ] }, "pscc": { "uniref50_id": "UniRef50_P47308", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47308" ], "product": "PTS system fructose-specific EIIABC component" } }, "id": "APKECPOBEN_209", "locus": "GCA002090215_00169" }, { "type": "cds", "sequence": "contig_1", "start": 94500, "stop": 94619, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTAATTATTGTGCAGCAACGTTACATCTATAGAAAATTACCGCAAGCATTACAAGGCATTAAGAACATTCTCTTTATTCCGTTACTAGGAACGCTAGTCACAGCAGCCTTGTTCTGA", "aa": "MLIIVQQRYIYRKLPQALQGIKNILFIPLLGTLVTAALF", "aa_hexdigest": "4a6316b7bf7b970071d88b097025d84a", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4471.4836000000005, "isoelectric_point": 10.446408653259276 }, "id": "APKECPOBEN_210", "locus": "GCA002090215_00170" }, { "type": "cds", "sequence": "contig_1", "start": 94644, "stop": 95273, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAACTATGGTCTGTCCAAGTTCTTGCAAATTATGGACAAACCATATTTGGCTCCCTTACTTGGTTTAGTGATTGGACTCATGATGTGCTTTGACTTGGGTGGACCGGTCAACAAAGCGGCCTATGTGTTTGGGGTAGTTTCGCTAGAGAGCCAAAACAGTGGTACGGTTGCAATGGCTAGTGCTATCTTATCGGGGATGGTGCCACCATTGGGTATTGCCATTGCTGCGACCATTCGCAAGCAGTGCTTTGACAAGGAAGAATTACCAGCAGCGTATGCTTGTTATGTGATGGGATTAAGTTTTATTAGTGAAGGGGCGATTCCTTTTGTTGCTAAACGGCCTAAGATTATGTTAGCTGCTAACCTAATTGGTGGTGCTGTGTGTGGTGTTTTAACGGGAGCATTTGCGCTAACGATTCGTGCCCCTCATGGTGGCGTGTTTGTCTTTGCCTTATTGAAAACTAACTTAGAGGGCATTGCCGGCAACACACTCCAAATCGGCGCTGGGGTTGGCTTAGCACTTCTAGCGCTAATAGTATCCAGCTTCATTAGTGCTGGCATTATTATTGGTCATAACCTGCTTGTAGTGCGTAAAAAAACAAAACAACTAGTAAATACCAATGCTTAA", "aa": "MNYGLSKFLQIMDKPYLAPLLGLVIGLMMCFDLGGPVNKAAYVFGVVSLESQNSGTVAMASAILSGMVPPLGIAIAATIRKQCFDKEELPAAYACYVMGLSFISEGAIPFVAKRPKIMLAANLIGGAVCGVLTGAFALTIRAPHGGVFVFALLKTNLEGIAGNTLQIGAGVGLALLALIVSSFISAGIIIGHNLLVVRKKTKQLVNTNA", "aa_hexdigest": "d8d4f8ca229ac002fca436f86be1a120", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 21653.737600000008, "isoelectric_point": 9.38996524810791 }, "id": "APKECPOBEN_211", "locus": "GCA002090215_00171" }, { "type": "cds", "sequence": "contig_1", "start": 95353, "stop": 96168, "strand": "+", "frame": 1, "gene": "fruK", "product": "Putative 1-phosphofructokinase", "db_xrefs": [ "COG:COG1105", "COG:G", "EC:2.7.1.56", "GO:0005524", "GO:0005829", "GO:0008662", "SO:0001217", "UniRef:UniRef50_Q49396", "UniRef:UniRef90_P75038" ], "nt": "GTGTTAACACCCGGTGGTAAGGGAATTAACATGGCGATTGTAATGGCTTTATTTGGCATCAAACCAACCGTGTTAACCTTTTTGGGTCAACCTACTAAAGATTTATTCTTGCAGTTGTTAAAACCGTACCAACTGGACTTAGTTAGCTTTCCAGCGACTACCCAAACACGGATTAACGTGAAGCTGTTGGATGGTGCCCAAACAACTGAAATTAACGATGTCACCCCGTTAATTGAGGAACAAGCAGTCCATGAAATGATTGCTTACCTTAAGGCTAACGTTAAGCCCAATGACCTGTTGGTGTTGAACGGCCGTTTTTTACAACGCGATTTAGTTAAGTTGTTAGATGTTGCTTTTAGCTTAACCAAGTATGTTGTATTAGATGTTGATGAACCTCAACTATTACAACTGTTAAACCAGCGCCAACCCTGGTTAATGAAACCGAACCGTGATGAGTTTGTAGCAATGGTTAATGCAAATAACAGTAACGTTGACCAACAAGAGTTGGTGCAGTTAATAAAGCAGTTTCAGACTACACAGAACTTATTAATGTCAGACGGCGCGCAGGGTGCTTACTTCTTTGATCAACAGCAACTGTTATTTATGGAAGCGATTCCTCCCCAACAGTTAGTTTCCACTACGGGAGCGGGAGATACATTGCTGGGTGTTTTCCTAGCTAACTTACTGTTAGATAAGGATCCAGTGGGTAGTTTAAAGGTAGCGGTTAACTATGCTAGTGCTACGATTAGCAAGCTCGCTGTGGTCAACAGTAATGACCAAATAGTGCTAAAAGCTACTAATTATTACTATTTATAA", "aa": "MLTPGGKGINMAIVMALFGIKPTVLTFLGQPTKDLFLQLLKPYQLDLVSFPATTQTRINVKLLDGAQTTEINDVTPLIEEQAVHEMIAYLKANVKPNDLLVLNGRFLQRDLVKLLDVAFSLTKYVVLDVDEPQLLQLLNQRQPWLMKPNRDEFVAMVNANNSNVDQQELVQLIKQFQTTQNLLMSDGAQGAYFFDQQQLLFMEAIPPQQLVSTTGAGDTLLGVFLANLLLDKDPVGSLKVAVNYASATISKLAVVNSNDQIVLKATNYYYL", "aa_hexdigest": "da752c3aaf00cb2d3c266e106be56649", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75038", "query_cov": 1.0, "subject_cov": 0.9033333333333333, "identity": 0.996, "score": 518.0, "evalue": 5.33e-185, "valid": true, "gene": "fruK", "product": "Putative 1-phosphofructokinase", "ec_ids": [ "2.7.1.56" ], "uniref50_id": "UniRef50_Q49396", "cog_id": "COG1105", "cog_category": "G", "go_ids": [ "GO:0005829", "GO:0008662", "GO:0005524" ] }, "pscc": { "uniref50_id": "UniRef50_Q49396", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49396" ], "product": "Putative 1-phosphofructokinase" }, "genes": [ "fruK" ], "id": "APKECPOBEN_212", "locus": "GCA002090215_00172" }, { "type": "cds", "sequence": "contig_1", "start": 96176, "stop": 96337, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_014574865.1", "SO:0001217", "UniParc:UPI0001DD64A0", "UniRef:UniRef100_A0AB38W8P1", "UniRef:UniRef50_A0AB38W8P1", "UniRef:UniRef90_A0AB38W8P1" ], "nt": "ATGTCAACTAAAGAACTAGCCAAACAGCCAAAGGTCAATCAAGAGAGGGAATCGATTTTGTTACCCGTTTTGCTAGCTGTTTTCAGTATGCTCTTCTTTATGCTACTGTTAGCTTTAGTGTTGTTGTACGCTATTCCAGGTAAAGTTAATAACTTGAACTAG", "aa": "MSTKELAKQPKVNQERESILLPVLLAVFSMLFFMLLLALVLLYAIPGKVNNLN", "aa_hexdigest": "d439b785fba30d91bfa41ab139de3492", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD64A0", "ncbi_nrp_id": "WP_014574865.1", "uniref100_id": "UniRef100_A0AB38W8P1", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD64A0", "RefSeq:WP_014574865.1", "UniRef:UniRef100_A0AB38W8P1" ] }, "ips": { "uniref100_id": "UniRef100_A0AB38W8P1", "uniref90_id": "UniRef90_A0AB38W8P1" }, "psc": { "uniref90_id": "UniRef90_A0AB38W8P1", "uniref50_id": "UniRef50_A0AB38W8P1" }, "pscc": { "uniref50_id": "UniRef50_A0AB38W8P1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W8P1" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5973.246899999998, "isoelectric_point": 9.398732948303223 }, "id": "APKECPOBEN_213", "locus": "GCA002090215_00173" }, { "type": "cds", "sequence": "contig_1", "start": 96405, "stop": 97511, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCGCAACTTAATTAAAAACGTTTTTCGCTCTTTTCGCACCGCTAAGATTGCCTTAATTGCGCTTACCTTTTTAATCTTTGTCGCGGTTGGTAGTTTCGTGTTATTAAACAATACCGTTAATAACTTTAACGCTTCCTTTCAACACGTTACCGAACAAGGACAATTGTCTAACGCCATTATTAACGAGCGCTATGACTTTGGTAAACTCGAGTTCCAAAAAACGAGTAATGATGCTACTAGTTCTGCCCAACCTAGTGTTAGTACTCGAAGTACTTTAACTGCCCAAAAAGCAAATGGCGCTAATTCCAGTTTTACTCTGACCTTAACACCTGACTCACGGACTTCATTTATTAACAACGCACTGCAACTAAAACCGGATGTTTATAACAGTTTAGTTACCGTTACTTTTAGTTATGGCAGTGAATCTGATCAAACCAAGAAAACCCAAATAGAAGAACAGTCCAAGTTAATTGCCGCAAACAATTTAAGCCGGGCATTACAAACCGATAAGGAGTTATTAGTTACGGGACAGTTAGAACAGTTAAAGGCTACCTTTCGCACTTATAAGGCAATTAACATTACTGATCAAGGAGTGTTCAAGAAGCTCATAGCCTCTGAACCGACCGATCAAGTTAACCGTCTCGTTCTGTTTGACGGACATCAACTAGCACGTTCCCGCCAAGTCGAATTTAACGAGCTGTTTAGTCAGTTTACCCAGGTGCAAGCTAAGGGTAAAGATCAGTTATCCACTACTCTAAAGAATGGTAATTACGAACAACTTTTGCAAGCGATCGCTAACAACTTTGGTAGTAGTGAAACTGAGTTACTTAAAGAAATTCAAGATGCTTTAAAGCAAACGAATAGTTCCACCACCCTTGCCCAAACCCAAAGTAGTCAACAATTTAGTAAGTTAAAAAACCTGTTTGATTCTGGTAGTACCTTTACTAACATTGCCGGCAAACTCACGTTGCAGTGGATGGAAGGTACAAACAAAAAGCAACTGGTAATCTTTGACCCGAGTAGCTATGAAACGGTAGTAGCACCGGGTAACTGGAGTTACGAACAGCAACAGGGCAAAGCGGTGTATCAGGACATTAACCACTGA", "aa": "MRNLIKNVFRSFRTAKIALIALTFLIFVAVGSFVLLNNTVNNFNASFQHVTEQGQLSNAIINERYDFGKLEFQKTSNDATSSAQPSVSTRSTLTAQKANGANSSFTLTLTPDSRTSFINNALQLKPDVYNSLVTVTFSYGSESDQTKKTQIEEQSKLIAANNLSRALQTDKELLVTGQLEQLKATFRTYKAINITDQGVFKKLIASEPTDQVNRLVLFDGHQLARSRQVEFNELFSQFTQVQAKGKDQLSTTLKNGNYEQLLQAIANNFGSSETELLKEIQDALKQTNSSTTLAQTQSSQQFSKLKNLFDSGSTFTNIAGKLTLQWMEGTNKKQLVIFDPSSYETVVAPGNWSYEQQQGKAVYQDINH", "aa_hexdigest": "eaffb92ca8970d4fa7e4f08849965612", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 41028.43790000003, "isoelectric_point": 9.000382804870608 }, "id": "APKECPOBEN_214", "locus": "GCA002090215_00174" }, { "type": "cds", "sequence": "contig_1", "start": 97581, "stop": 97838, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGGTCGACAAAATTGAATACCTCATCTTGGGAGTGGGAATTACCCCAGACTTTGTCTACCCCGTCTTTAGTGAATCACTGTTAATTCCCAATACGGCTAACGAGCAGTTGTATTATGTCAACCAAACTGGTTATGAACGTACCGCTAGCTCCTTTTTAACTAATCCCACCGAAACTGGCATAGTAGCACGGTTACCTAACCTCAACCATGACCTTGATGTAATTAACCAGTGGGCTGTTAAAAACATGTCCTGA", "aa": "MLVDKIEYLILGVGITPDFVYPVFSESLLIPNTANEQLYYVNQTGYERTASSFLTNPTETGIVARLPNLNHDLDVINQWAVKNMS", "aa_hexdigest": "2f3edb560e1258c6123ebab8a737b2cf", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9577.764099999995, "isoelectric_point": 4.395041084289551 }, "id": "APKECPOBEN_215", "locus": "GCA002090215_00175" }, { "type": "cds", "sequence": "contig_1", "start": 97980, "stop": 98297, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02687.27" ], "nt": "GTGGCCATCTTTGTCACCATTTTAATCTTGGTTAGTTACCTCAAAAAGAACACGGAACAAATCGGCATCTTAAAGGCTAATGGGGTGAGTGGCCGGAAGATTAACTTAAGCCTGTTAATCTTTAGTTTAATCCCGGGGATAGTGGGGTCCTTTATTGGTTATACGTTAGGTATTAGTATGCAAAACGTGGCCATTAACCTCTTTAGTAACTACTGGTTTATCCCCACGCAAACTTCGAGTTTCTCGGTGTTAGGGTTACTTTTCTTTGCCTTATTAGTGGTTCTTATGATGAGTGGGATTAGTCTGTTTGTGGGGTGA", "aa": "MAIFVTILILVSYLKKNTEQIGILKANGVSGRKINLSLLIFSLIPGIVGSFIGYTLGISMQNVAINLFSNYWFIPTQTSSFSVLGLLFFALLVVLMMSGISLFVG", "aa_hexdigest": "5f22642b17d0925359a7c7a29844e4e6", "start_type": "GTG", "rbs_motif": "AACAA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02687.27", "name": "FtsX", "length": 102, "aa_cov": 0.9619047619047619, "hmm_cov": 1.0, "evalue": 6.196403665646943e-16, "score": 54.036476135253906, "start": 2, "stop": 102 } ], "seq_stats": { "molecular_weight": 11409.65179999999, "isoelectric_point": 9.873542594909669 }, "id": "APKECPOBEN_216", "locus": "GCA002090215_00176" }, { "type": "cds", "sequence": "contig_1", "start": 98403, "stop": 98618, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACCAGATTTCGTGTCGCTTTAGCCTTTAATGCGCCGTGGAAACTGGTCTTTTTAACCTTAATGAGTGCACTAACGATGATGATCCTAAACCTTAGTTTTGCCACTAAGGATGCCTTTCAGGTAGCACAAACAAAGACTACTTTAACCCAATCGAACCACCAGTACGAGTTTGAACTAGCATCGCCAACGGAGCAGAGTGGCTTGCTCAAGTGA", "aa": "MTRFRVALAFNAPWKLVFLTLMSALTMMILNLSFATKDAFQVAQTKTTLTQSNHQYEFELASPTEQSGLLK", "aa_hexdigest": "721e075f6949e99f2db9ca17c102f9d1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8043.3189999999995, "isoelectric_point": 9.397636985778806 }, "id": "APKECPOBEN_217", "locus": "GCA002090215_00177" }, { "type": "cds", "sequence": "contig_1", "start": 98817, "stop": 99419, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGACTATTCACTGGGTTTTGGTAACGTTTCTTCCAATCCGTGGAAGCTGAGTGAAACATTAATGCCCGCCAATCAAGCATCCGCTTCCAACACGGCTTTCCAAAACTTTTTAAAGGCGATTATTGTAATTGACCCTAACGAGGGCGGTAAGTACATTAAACAAACTAAGGATGAAGTTACTGTGCGTTTTTTATACGAAATAGACAGTAGTAAAGCTTTAAAAAACGGCAACACCAAATCAGTTAATAAAATACAAAAAAGTACCGCAGCACCCCAAGCCCAACAAGGACAAAGTAACCAGTTAATGCTTGACAATGATTTTGCTAAATTCTTGTACAAGCAGTTTAACCTAATCAAGGAAGGGGGCAAAGCAGATCCGGTTGATCTCAATAACTTTGACTTTGCTAATCAGAATCAGATTAAGCGTTTTTACAGTAAATACAACGCCTTACCACCGGTAGATTACAAGCTGGCCTTTAACGTGATCGGTCTCCCTAAAGAAACAATTGCCAACCAAAAGGATGCGCCCCTGTTTGGCTTTTTAACCTTGCAAGCGCAACTGGGCAATGACGCGATTAAGATTAAGGGGATTAAAGACTGA", "aa": "MDYSLGFGNVSSNPWKLSETLMPANQASASNTAFQNFLKAIIVIDPNEGGKYIKQTKDEVTVRFLYEIDSSKALKNGNTKSVNKIQKSTAAPQAQQGQSNQLMLDNDFAKFLYKQFNLIKEGGKADPVDLNNFDFANQNQIKRFYSKYNALPPVDYKLAFNVIGLPKETIANQKDAPLFGFLTLQAQLGNDAIKIKGIKD", "aa_hexdigest": "2813de51a8f8e0d84be81da9514a6e04", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 22253.004199999996, "isoelectric_point": 9.204554176330568 }, "id": "APKECPOBEN_218", "locus": "GCA002090215_00178" }, { "type": "cds", "sequence": "contig_1", "start": 99447, "stop": 100565, "strand": "+", "frame": 3, "gene": null, "product": "ABC transporter permease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0011815B2F", "UniRef:UniRef90_UPI0011815B2F" ], "nt": "GTGTTGCGTACCAATGAGGACGAGATTATTAACCAGCGCTTGTTTAGTAAACCCAACGGTATTATTATCAACAGTTCAGCTGCTAAAAAGTACCGCTTAAAGGAAGGTAGTCAGCTCCATGTCAAAATTCACAACGGTTACAAACGAGTGAATGCTAAGTTAGTTAACCAAGATCCCACTTTAACTGCCACCTTTAACATTGTTGGCATTAACAATTCGGTGCACGAACCGGAGTTTTACACGAGTTACAAAACGGCAGCAACACTATTGCAATACCCCAATGAATGGATCGAAAAGCAGTTACCCTTTAACAGCTTTTATGCCAATTCGATCTTGCCCTTTACCCAGTCTACTTCCCTCTTTTCCGAATCTGGCTTATTCCCTGGTACCAGTAGCTTTGCCGCTAACAACACGGTGCTTACTGAAGTAATTAAGAAAACGATTGAAGGTGGTAAAACTAACAGTAAAACTGATGTGCAAAAGCGCGCTGCTCAAGCTACTGCTAACGGTAAGTATAACAGTAGTCAAAACAACAAGATGAATTATGAGCGCTTACAAAAGGCACTGGGCATTACCAGTGATTTAGATAGCTCTAAAGCCGGTGAATACGCCACCATCTTAGAGCGGGTGTATGATGGTTTGCCATACAACTCCACTATTAGCTTTATTAGTAATGTGCAGGCCAACGATGCCTTGTTTGCTAACATTGCTAACACCACCCAGCAAATCCAAACAGCGGCCATTGGCATTATTATTCCGATTATTATGCTAATTGTGTTGTTAGTATCAACTACCCTAATTCAGGAATTGAAGAAGATTGCGATTCGTTTAAAAGCATTGGGCTATTCCAACCCGAAGATCTTGCTGTCCTTCTTGTCGATTTACCTCCCGTTATTTATCTTTGGTTTGTTAATTTCGATCCCAATTTCGATTTATCTAATTGCACTCCACAATCAAGTTATTTTCTCCAGTGCTGCCATCTTACTGGAAGCCACATTAAACTTCCCCACGGTCATTCTGTCGATGTTAGTGTTAAGTGGGGTGTTATCAATTACCTTTGTGTTGAATTGGTTGGAGTTGAACAAAATCAAAATTGCCAAGGAGATTAAGAATAGCTAA", "aa": "MLRTNEDEIINQRLFSKPNGIIINSSAAKKYRLKEGSQLHVKIHNGYKRVNAKLVNQDPTLTATFNIVGINNSVHEPEFYTSYKTAATLLQYPNEWIEKQLPFNSFYANSILPFTQSTSLFSESGLFPGTSSFAANNTVLTEVIKKTIEGGKTNSKTDVQKRAAQATANGKYNSSQNNKMNYERLQKALGITSDLDSSKAGEYATILERVYDGLPYNSTISFISNVQANDALFANIANTTQQIQTAAIGIIIPIIMLIVLLVSTTLIQELKKIAIRLKALGYSNPKILLSFLSIYLPLFIFGLLISIPISIYLIALHNQVIFSSAAILLEATLNFPTVILSMLVLSGVLSITFVLNWLELNKIKIAKEIKNS", "aa_hexdigest": "69c4999d968af0e54fcecd287fbf5013", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0011815B2F", "query_cov": 1.0, "subject_cov": 0.8551724137931035, "identity": 0.992, "score": 674.0, "evalue": 1.26e-242, "valid": true, "product": "ABC transporter permease", "uniref50_id": "UniRef50_UPI0011815B2F" }, "pscc": { "uniref50_id": "UniRef50_UPI0011815B2F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0011815B2F" ], "product": "ABC transporter permease" }, "genes": [], "id": "APKECPOBEN_219", "locus": "GCA002090215_00179" }, { "type": "cds", "sequence": "contig_1", "start": 100565, "stop": 101956, "strand": "+", "frame": 2, "gene": "lolD", "product": "Putative ABC transporter ATP-binding MG065-like protein", "db_xrefs": [ "COG:COG1136", "COG:M", "GO:0005524", "GO:0016887", "SO:0001217", "UniRef:UniRef50_P75612", "UniRef:UniRef90_P75612" ], "nt": "ATGCGATACTTTGCCCTCACTGATTTAACCAAGCCCACTGCAAAATTTACCACTGATGCCATGGAACTGCGTCAGATATCCAATGCTTACCTCAACCAAGCCCAAGCATATTTGCAAAAGGGTTTAAAGCAACTCAAAAAGGATTACAAGAATGCCATTTTGTATACCCCCAAAACGGAGTACAAGCGCTTTTTGAAATGGAAGCAAACTTTTCTTCAAGATTTAAACCAAACCCAAAAGCGCTTCTTTATTGTTCGTGCCCAACATTTTTCTTACGTGTTGTTGTTTAACTTATTGGATGAACAGGTGGAAGCGGTGATTGCGACCTTTAACAACTTCTTGGATGAACACCGCTTGGAGGCACAATCCAAGCAGTTTGATTTAGACACAGCTGTCAATGAACTGCATGATTACTTTGACCAACTGCAAAAGAACACCGCTTACAGCGAGGATTTACCAACTCACCTCACGCAAAAAACCGAGCAATTAATTAACGCTCGTAATACCCAGTTAACGAATTTGTTAAACAAGATTGCTACTACCAAACCACCCCTAAACAAACAGCAACGTTTGTTAGCCAGTTTTCGCAATTACCACGAACACCTCTTTTTAAAGAACGAGGTCAAAAAGGTGACTTGGTTAAACGAACCACGCGCCAAAAAAGAGTCTGTTACCCCTGATGAAGAACACATTATTGAACTTAAAAACGTTTACAAGTACATTACTAATGGGGTGACTACCAATGCCGTTTTAAAGGGCATAGATCTCAAACTCAAAGCCCATGATTTCATTGTGATTTTAGGTCCCTCTGGCTCGGGGAAAACGACCCTGCTCAACATTATTTCCGGGATGGACCGGCCATCAAGCGGATCTGTTGTCGTTAATGGTCAGGAAATGATTTGCATGAACGACCGTCAGTTAACTAACTTTCGCCGCAACTATGTCGGTTACATCTTCCAGCAGTACGGGCTTTTACCCAACCTAACTGTAAGGGAAAACGTTGAAGTGGGTGCTAACTTGCAACGTAACCCTGATAAGCGGATTAACATTGATGAACTGTTAGAAGCAGTAGGGATGAAACACCTCCAAAAGAAACTCCCCAACGAGTTGAGTGGTGGACAACAACAACGGGTCTCGATTGCACGGGCGTTTGCTAAAAATCCCTTACTCATTTTTGGTGATGAACCAACTGGGGCTTTGGATTTGGAAATGACCCAAATTGTGCTCAAACAGTTTTTAGCCATTAAACAACGCTATAAAACCACGATGGTCATAGTGACGCACAATAACCTAATTGCCCAACTAGCCGATTTAGTCATTTATGTGGCAGATGGCAAAATTCAAGCACTGCAAGCAAACCCTAATCCCAAACAGGTTGAGGACATTAACTGA", "aa": "MRYFALTDLTKPTAKFTTDAMELRQISNAYLNQAQAYLQKGLKQLKKDYKNAILYTPKTEYKRFLKWKQTFLQDLNQTQKRFFIVRAQHFSYVLLFNLLDEQVEAVIATFNNFLDEHRLEAQSKQFDLDTAVNELHDYFDQLQKNTAYSEDLPTHLTQKTEQLINARNTQLTNLLNKIATTKPPLNKQQRLLASFRNYHEHLFLKNEVKKVTWLNEPRAKKESVTPDEEHIIELKNVYKYITNGVTTNAVLKGIDLKLKAHDFIVILGPSGSGKTTLLNIISGMDRPSSGSVVVNGQEMICMNDRQLTNFRRNYVGYIFQQYGLLPNLTVRENVEVGANLQRNPDKRINIDELLEAVGMKHLQKKLPNELSGGQQQRVSIARAFAKNPLLIFGDEPTGALDLEMTQIVLKQFLAIKQRYKTTMVIVTHNNLIAQLADLVIYVADGKIQALQANPNPKQVEDIN", "aa_hexdigest": "08a651a5d28d33956c8c41142d0fd463", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75612", "query_cov": 1.0, "subject_cov": 0.9956989247311828, "identity": 1.0, "score": 894.0, "evalue": 0.0, "valid": true, "gene": "LolD", "product": "Putative ABC transporter ATP-binding protein MG065 homolog", "uniref50_id": "UniRef50_P75612", "cog_id": "COG1136", "cog_category": "M", "go_ids": [ "GO:0005524", "GO:0016887" ] }, "pscc": { "uniref50_id": "UniRef50_P75612", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75612" ], "product": "Putative ABC transporter ATP-binding protein MG065 homolog" }, "genes": [ "lolD" ], "id": "APKECPOBEN_220", "locus": "GCA002090215_00180" }, { "type": "cds", "sequence": "contig_1", "start": 101983, "stop": 102597, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00456.26" ], "nt": "ATGAAAAACCTGTTTGCTTGCCAACACTTAGCGTTAAGTGCTATTCAACACGCTAAGGGTGGTCATGTGGGAATGGCCTTAGGTGCTAGTCCTATTTTGTACACCTTATGGACTAAACACATCCAGTTTAACCCTAACTGTCCCAAATGGATTAACCGGGACCGTTTAGTGATGAGTGCAGGTCATGGTAGTATGGCCTTATACCCGATCTTACACTTTGCGGGGTTAATTACGAAGCAGGAAATGCTCCATCACAAATACGGGCAAGTCAACACCTCTTCCCATCCCGAGTATGCCCCTAATAACTTTATTGATGCGTCCACTGGTCCCTTAGGACAAGGCCTAGGAATGGCTGTCGGGATGGCCTTGACACAACGGGTACTAGCAGCTGAATTTAAAGCGTTAAGTCCCAAACTGTTTGACCACTTTACCTATGTGGTTGTGGGTGATGGTGATCTCCAGGAAGGGGTGAGTTATGAGGTAGCTCACTTAGCAGGGGTCTACCAGTTAAATAAGCTAATAGTGCTCCATGATTCCAACCGGGTGCAGATGGACAGTGTCGTGCGTGATGTGTCCTTGGAAAACCTGCAAACCCGGTTTACCAACATGGGGTGA", "aa": "MKNLFACQHLALSAIQHAKGGHVGMALGASPILYTLWTKHIQFNPNCPKWINRDRLVMSAGHGSMALYPILHFAGLITKQEMLHHKYGQVNTSSHPEYAPNNFIDASTGPLGQGLGMAVGMALTQRVLAAEFKALSPKLFDHFTYVVVGDGDLQEGVSYEVAHLAGVYQLNKLIVLHDSNRVQMDSVVRDVSLENLQTRFTNMG", "aa_hexdigest": "2e6a12f53cfc188c3415e6b1af99b6bb", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00456.26", "name": "Transketolase_N", "length": 198, "aa_cov": 0.9656862745098039, "hmm_cov": 1.0, "evalue": 1.7786343558716266e-62, "score": 206.6949920654297, "start": 7, "stop": 203 } ], "seq_stats": { "molecular_weight": 22438.606300000003, "isoelectric_point": 7.886175727844237 }, "id": "APKECPOBEN_221", "locus": "GCA002090215_00181" }, { "type": "cds", "sequence": "contig_1", "start": 102685, "stop": 102939, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCACACGACAATTGCTAAAAACACCACCTTGGAAGACCAACCCGCTGGTCACTGGTTTATTCCTACCGACAAGGACTTTGCGCGCTTTAACAGCAACACCAAGACTAACTTTACCCCGTTTGAATATCCCCAAACGGTGTATGACTTCTTTCACAAGCAGGTCATAGCACGCCAAGCTAAACCAGTGCAAGCTTACAAGGAATTACTGGAAAAACTAAAGGACAAACCGCTGTATACTAAGTTTATTAATTGA", "aa": "MHTTIAKNTTLEDQPAGHWFIPTDKDFARFNSNTKTNFTPFEYPQTVYDFFHKQVIARQAKPVQAYKELLEKLKDKPLYTKFIN", "aa_hexdigest": "8e35bb182d4d48773d49830a5ca94b70", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9954.206299999994, "isoelectric_point": 9.222992134094238 }, "id": "APKECPOBEN_222", "locus": "GCA002090215_00182" }, { "type": "cds", "sequence": "contig_1", "start": 102961, "stop": 103413, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02779.29" ], "nt": "TTGTACCTCAACCAGTTAGATGAACGCAAGGTAGCACAAGCCAATGCGGCAACCCGTAACTACCTCAAGGACTTTTTAGGCCAAATTAACAACAGTAATTCCAACTTGTACTGTCTCAATGCTGATGTAGCACGTTCGTGTAACATTAAGTTAGGTGATGATAATTTACATACCAATCCGCATTCGCGCAACATTCAGGTGGGTATTCGGGAATTTGGTATGTCCACCATTATGAACGGGATGGCACTCCATGGTGGGGTCAAAGTGATGGGTGGTACCTTCCTAGCGTTTGCTGATTATTCCAAACCCGCAATTCGCTTAGGGGCGTTAATGAACCTCCCCACCTTTTATGTGTACACCCATGACTCTTACCAAGTCGGTGGTGATGGACCAACCCACCAACCGTACGATCAACTCCCGATGTTAAGGGCCATTGAAAACGTTCAGGTTTGA", "aa": "MYLNQLDERKVAQANAATRNYLKDFLGQINNSNSNLYCLNADVARSCNIKLGDDNLHTNPHSRNIQVGIREFGMSTIMNGMALHGGVKVMGGTFLAFADYSKPAIRLGALMNLPTFYVYTHDSYQVGGDGPTHQPYDQLPMLRAIENVQV", "aa_hexdigest": "dbe660a45fe775ba2c7ba0f68ec795c1", "start_type": "TTG", "rbs_motif": "AAAA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02779.29", "name": "Transket_pyr", "length": 137, "aa_cov": 0.9, "hmm_cov": 0.9927007299270073, "evalue": 5.7481485243838224e-36, "score": 119.21614837646484, "start": 16, "stop": 150 } ], "seq_stats": { "molecular_weight": 16639.69429999999, "isoelectric_point": 6.952681922912597 }, "id": "APKECPOBEN_223", "locus": "GCA002090215_00183" }, { "type": "cds", "sequence": "contig_1", "start": 103459, "stop": 103929, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF22613.1" ], "nt": "TTGTTAAGCCAAGACCAAACTAACGTATTAATTTTGACCCGTCAAGCTTTGCCTTCCCTAGAACAATCTGATAGTGTACAGACCTTGAAAGGGGGCTATATTATTAGTAATAGAAAACAACCCGATGTCATTGTGGCCGCTAGTGGCAGTGAAGTTCAGCTGGCATTGCAACTAGAACAAGCTCTAAACGAACAACAATTAAAGACGCGGGTGGTGTCAGTGCCCAACATTAACATGTTGTTAAGTCAACCCCAAAGCTACTTGCAACAACTGTTTGATCCTAACAGTGTTTTACTCACCTTAGAAGCGAGTGCTAGCATGGAGTGGTATGCACTAGCAAAATACGTCAAAAAGCACACCCACTTAGGTGCTTTTAGCTTTGGTGAGTCCAATGACGGGCAAGTCGTGTATGAACACAAGGGCTTTAATGTGACTAACTTACTCAAGTTGATTAAAACTCTAAAGAGTTAA", "aa": "MLSQDQTNVLILTRQALPSLEQSDSVQTLKGGYIISNRKQPDVIVAASGSEVQLALQLEQALNEQQLKTRVVSVPNINMLLSQPQSYLQQLFDPNSVLLTLEASASMEWYALAKYVKKHTHLGAFSFGESNDGQVVYEHKGFNVTNLLKLIKTLKS", "aa_hexdigest": "5a3123250bc381e5427d9d4d9ec847a7", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF22613.1", "name": "Transketolase_C_1", "length": 115, "aa_cov": 0.7243589743589743, "hmm_cov": 0.9826086956521739, "evalue": 4.093914836305563e-28, "score": 93.36075592041016, "start": 31, "stop": 143 } ], "seq_stats": { "molecular_weight": 17330.6251, "isoelectric_point": 6.829852867126465 }, "id": "APKECPOBEN_224", "locus": "GCA002090215_00184" }, { "type": "cds", "sequence": "contig_1", "start": 104485, "stop": 104613, "strand": "+", "frame": 1, "gene": null, "product": "DUF31 family putative serine protease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F4E8A2E", "UniRef:UniRef90_UPI001F4E8A2E" ], "nt": "GTGATTGAGCAGAAGCTCAAGGAAGATTCGCTGTTGTTTATCCAAACGAGTACGTTACCTAAAACAGCATACGCAGCAATTGATCCGGTGAACTTTAGCTATAACCCAACCCGTACCAACGGTTTTTGA", "aa": "MIEQKLKEDSLLFIQTSTLPKTAYAAIDPVNFSYNPTRTNGF", "aa_hexdigest": "2f1356b19045b9a466b124ba6dfd607d", "start_type": "GTG", "rbs_motif": "AAAA", "genes": [], "truncated": "both", "pseudogene": { "start": 104369, "stop": 104624, "observations": { "deletion": [ 104402, 104609 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001F4E8A2E", "query_cov": 1.0, "subject_cov": 0.49411764705882355, "identity": 0.976, "score": 85.5, "evalue": 6.18e-21, "gene_start": 1, "gene_end": 42, "reference_start": 40, "reference_end": 81, "reference_sequence": "MVGVNHILTSKKTQSFLLGKYTKPNVQLVKTNYEKPEDAVIEQKLKEDSLLFIQTSTLPKTAYAAIDPVNFSYNPTRTNGFLNSR" }, "paralog": false, "identity": 1.0, "score": 160.0, "evalue": 8.43e-53, "description": "Frameshift due to deletion around 104402, 104609.", "psc": { "uniref90_id": "UniRef90_UPI001F4E8A2E", "product": "DUF31 family putative serine protease", "uniref50_id": "UniRef50_UPI001F4E8A2E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001F4E8A2E", "UniRef:UniRef50_UPI001F4E8A2E" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001F4E8A2E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F4E8A2E" ], "product": "DUF31 family putative serine protease" } }, "id": "APKECPOBEN_225", "locus": "GCA002090215_00185" }, { "type": "cds", "sequence": "contig_1", "start": 104677, "stop": 105201, "strand": "+", "frame": 1, "gene": null, "product": "DUF31 family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000AB22BFD", "UniRef:UniRef90_UPI000AB22BFD" ], "nt": "GTGATTGAAGTACCGTTAGTACTATCCAACCCCAATGATGCCAAGATCTACCAAGAGTGGATTAGACCAGCTACCCAAGCCTACAAATATTTAGGTGATGTAGAAGGTTTGTTTGCTAAGAAGGGCTATCGCAGTTACATCCAAGATTTCTACCACTTATTGGGTTATCCGGTAACTAAGAACACTAAATCAGAGTTTATTTTGGGACAAAGTCAAGGTACTGTGAACCACATGAGTTTTAGTAATGATGAGTCCAATACTACTAATACTATTACTAAAGCTAGCTTAACTCAGCAACCCCACAAGGAACAATCAGCTTACGTGGTACGTGAAAGTGGACTGCCAACCCTAACAATGAATGTGGACAAGTACACTGGTGCAAAGGGAACTCACCTAGTTAACGTTGACCAAATTACTGACCTTAGTTTGGGTGATGGTTTAATTGACTTTGGTGGATTATCACGCTTTATCTTGCAGTACCACAATGTGAACTATAAGCAATTTGGTTATGGCACGATCTTGTGA", "aa": "MIEVPLVLSNPNDAKIYQEWIRPATQAYKYLGDVEGLFAKKGYRSYIQDFYHLLGYPVTKNTKSEFILGQSQGTVNHMSFSNDESNTTNTITKASLTQQPHKEQSAYVVRESGLPTLTMNVDKYTGAKGTHLVNVDQITDLSLGDGLIDFGGLSRFILQYHNVNYKQFGYGTIL", "aa_hexdigest": "3cb5f5faca0d6a4e7f2e23a9e0102508", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 104379, "stop": 105504, "observations": { "insertion": [ 104403 ], "stop": [ 104611, 105199 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000AB22BFD", "query_cov": 1.0, "subject_cov": 0.4496124031007752, "identity": 0.9940000000000001, "score": 349.0, "evalue": 1.4e-118, "gene_start": 1, "gene_end": 174, "reference_start": 101, "reference_end": 274, "reference_sequence": "MGSTISLPATQSFLLGKYTKPNVQLVKTNYEKPEDAVIEQKLKEDSLLFIQTSTLPKTAYAAIDPVNFSYNPTRTNGFWTAGKYNVYNGGNSIGNYADFAVIEVPLVLSNPNDAKIYQEWIRPATQAYKYLGDVEGLFAKKGYRSYIQDFYHLLGYPVTKNTKSEFILGQSQGTVNHMSFSNDESNTTNTITKASLTQQPHKEQSAYVVRESGLPTLTMNVDKYTGAKGTHLVNVDQITDLSLGDGLIDFGGLSRFILQYHNVNYKQFGYGTILWDTNFAGGSSGSAIFNQNKQINSIYFGALVNVTTDRNENVGLGLGQILRAPNTFNSSHEVPYSYDLIFGDVNTTNFYAQFAKKHNTHMWSKIQSTQNGEIGFHKNSKTGQQRH" }, "paralog": false, "identity": 0.9919786096256684, "score": 739.0, "evalue": 1.05e-274, "description": "Internal stop codon at 104611, 105199. Frameshift due to insertion around 104403.", "psc": { "uniref90_id": "UniRef90_UPI000AB22BFD", "product": "DUF31 family protein", "uniref50_id": "UniRef50_UPI000AB22BFD", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000AB22BFD", "UniRef:UniRef50_UPI000AB22BFD" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000AB22BFD", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000AB22BFD" ], "product": "DUF31 family protein" } }, "id": "APKECPOBEN_226", "locus": "GCA002090215_00186" }, { "type": "cds", "sequence": "contig_1", "start": 105316, "stop": 105540, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGGTTTAGGCTTAGGGCAAATTCTCCGTGCTCCCAATACCTTCAACTCTTCCCACGAAGTGCCCTATTCTTATGACCTCATCTTTGGTGATGTTAACACCACCAATTTTTACGCCCAGTTTGCGAAAAAGCACAACACCCACATGTGGAGCAAAATTCAAAGTACGCAGAATGGAGAAATTGGTTTCCACAAAAATTCAAAAACCGGTCAACAGCGGCATTAA", "aa": "MGLGLGQILRAPNTFNSSHEVPYSYDLIFGDVNTTNFYAQFAKKHNTHMWSKIQSTQNGEIGFHKNSKTGQQRH", "aa_hexdigest": "e9707c9fa9ae8863d20c8fdbe5f5697e", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8426.264599999999, "isoelectric_point": 9.522254371643069 }, "id": "APKECPOBEN_227", "locus": "GCA002090215_00187" }, { "type": "cds", "sequence": "contig_1", "start": 105590, "stop": 106132, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01732.22" ], "nt": "ATGTTACTCCGCTCTGTTTGGTATAAGCTTGGTAGTCTGTTAATCATCTTACCGCTAACGGGTTGTGGTTATGTCAGACTAAAGCGTGCTAATTTCCAAACAGACTTTACCATTAACCGCATTCCCACCCAAGGGGATGTGTACCATGATAACTATGACCTGACTTTTTCACTCAACTTTGCTACTAGTTCCAAAGATTCTTATGGTACGGGTTGGTTAATTGACTGGAAGGGTGATGAGAATAAGCCTAGCAATACCGATCCCTTCCTGATTTACTTAGCGACTAACCTACACGTAGTCGATGCCTTGCGCAATCCCCAAGACTATGAACCTTACAATAAGGATAGTAATGGTCAGGACTATGGTAATCGTGATATAACCCATTTCTTTGCATTGGGTAAATACACTGATCCAGGTTTATTGGGTGTTGAAACAAAAGAGCAATCAGCCTTTATTTCGATTCAAACATCAGCAATCCCCAAAACCGCTTATACGGCTAATGATTTTGTTGATTATCAGTATGATAGTCTTACAAAACAGTGA", "aa": "MLLRSVWYKLGSLLIILPLTGCGYVRLKRANFQTDFTINRIPTQGDVYHDNYDLTFSLNFATSSKDSYGTGWLIDWKGDENKPSNTDPFLIYLATNLHVVDALRNPQDYEPYNKDSNGQDYGNRDITHFFALGKYTDPGLLGVETKEQSAFISIQTSAIPKTAYTANDFVDYQYDSLTKQ", "aa_hexdigest": "adadb7b863850d210c0b140423272ece", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01732.22", "name": "Mycop_pep_DUF31", "length": 148, "aa_cov": 0.7277777777777777, "hmm_cov": 0.9391891891891891, "evalue": 5.855054858998897e-37, "score": 123.53508758544922, "start": 46, "stop": 176 } ], "seq_stats": { "molecular_weight": 20493.577099999988, "isoelectric_point": 5.059659385681152 }, "id": "APKECPOBEN_228", "locus": "GCA002090215_00188" }, { "type": "cds", "sequence": "contig_1", "start": 106089, "stop": 106178, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTTTGTTGATTATCAGTATGATAGTCTTACAAAACAGTGAAATAAAAAGAGTGATCAAAAAGATCAAACTCAAGAACAAACGGTAA", "aa": "MILLIISMIVLQNSEIKRVIKKIKLKNKR", "aa_hexdigest": "66e4ad296bd39633a6903737f595a84a", "start_type": "ATG", "rbs_motif": "AAAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3464.411100000001, "isoelectric_point": 11.263674354553224 }, "id": "APKECPOBEN_229", "locus": "GCA002090215_00189" }, { "type": "cds", "sequence": "contig_1", "start": 106232, "stop": 107164, "strand": "+", "frame": 2, "gene": null, "product": "putative lipoprotein MPN_084", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P47314", "UniRef:UniRef90_P75609" ], "nt": "GTGCCATTGTTCTTAGACAACGTCCGTGACAAACAGGTCTTTGATTATTTTGTCAAACCAGCCATTGAGACCTATAAAAAGCTTGGTGATACGGCTCAATTATTTACTGAGCAAAACCTAAAGGAATTGGAAAAGGAAACCTATATTATGCTGGGCTATCCGGTAGTGGAGAGCAATATTTATGCCCACATTTTAGGTCAAGGAAAAGAGTTGCGTGTGACTCAACAAGTCAACAACCAACCAGTTAAAAAGAACTATGTTTTGCAAACCATTACTAAAGAGCAACACACTTTGGATATTACGCGTGAAATACCAACCTTAATCCAAAACAAGAGTTTATCGGGTGATTTTGTTGGTAGTAAGCTCTTATCTCAAGAAGAACAACAGCAAGAACATGCTTTTTTGGGTAACTTAAACCAAGGGATAATTGACTTTGCTAGATTGTCAAACTTTAACTTGCAGTACCACAACCGCGAATACCAACAATACGGTAAGGGTTTAGCATTAGCTAACACTAACTTTAGTGGTGGTTCATCCGGTACCTTAGTTTTAAATCAACAAAAACAAATTTCCGGGGTTTACTTTGGCGTGTTAGAGTTTGGTGGCACCAACGGCACTAACCGTGAAAGTAGTATTGGTGTTGGTCAAATTTTGCGAGTAAAAGATGACGCTCAACTCCAACAACACAGTCACAACAACTTACTTTCACAATTAGGTTCCAGTCATAACTCGATTACTTACGACATTATTTTTGGTAACAAAGACACTAAAAACTACTACGCCCAGTTTGCCAAAAAACATCAAACACATTTGTACAGCCAAATAAGTTCAAGTAATCAGCAAGAACTCAAATACGTCGATAACGATCCAAATCTCAAGATTAAAGAAGAGGCTGCCAAAAGCACTGTTCAGAATTTAACTGTCTACAGTTAA", "aa": "MPLFLDNVRDKQVFDYFVKPAIETYKKLGDTAQLFTEQNLKELEKETYIMLGYPVVESNIYAHILGQGKELRVTQQVNNQPVKKNYVLQTITKEQHTLDITREIPTLIQNKSLSGDFVGSKLLSQEEQQQEHAFLGNLNQGIIDFARLSNFNLQYHNREYQQYGKGLALANTNFSGGSSGTLVLNQQKQISGVYFGVLEFGGTNGTNRESSIGVGQILRVKDDAQLQQHSHNNLLSQLGSSHNSITYDIIFGNKDTKNYYAQFAKKHQTHLYSQISSSNQQELKYVDNDPNLKIKEEAAKSTVQNLTVYS", "aa_hexdigest": "0b1614891baa0c2f003b77c7375ee4f0", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 105932, "stop": 107164, "observations": { "mutation": [ 106130, 106190 ], "stop": [ 106130, 106190 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75609", "query_cov": 1.0, "subject_cov": 0.5893536121673004, "identity": 0.997, "score": 600.0, "evalue": 2.31e-213, "gene_start": 1, "gene_end": 310, "reference_start": 217, "reference_end": 526, "reference_sequence": "MPCLLRSVWYKLGSLLIILPLTGCGYVRLKRANFQTDFTINRIPTQGDVYHDNYDLTFSLNFATSSKDSYGTGWLIDWKGDENKPSNTDPFLIYLATNLHVVDALRNPQDYEPYNKDSNGQDYGNRDITHFFALGKYTDPGLLGVETKEQSAFISIQTSAIPKTAYTANDFVDYQYDSLTKQWNKKSDQKDQTQEQTVTGQSWSYKPAYADFAVIEVPLFLDNVRDKQVFDYFVKPAIETYKKLGDTAQLFTEQNLKELEKETYIMLGYPVVESNIYAHILGQGKELRVTQQVNNQPVKKNYVLQTITKEQHTLDITREIPTLIQNKSLSGDFVGSKLLSQEEQQQEHAFLGNLNQGIIDFARLSNFNLQYHNREYQQYGKGLALANTNFSGGSSGTLVLNQQKQISGVYFGVLEFGGTNGTNRESSIGVGQILRVKDDAQLQQHSHNNLLSQLGSSHNSITYDIIFGNKDTKNYYAQFAKKHQTHLYSQISSSNQQELKYVDNDPNLKIKEEAAKSTVQNLTVYS" }, "paralog": false, "identity": 0.9926829268292683, "score": 807.0, "evalue": 3.31e-297, "description": "Internal stop codon at 106130, 106190. Nonsense mutation around 106130, 106190.", "psc": { "uniref90_id": "UniRef90_P75609", "product": "Uncharacterized lipoprotein MPN_084", "uniref50_id": "UniRef50_P47314", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75609", "UniRef:UniRef50_P47314", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47314", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47314" ], "product": "Uncharacterized lipoprotein MG068" } }, "id": "APKECPOBEN_230", "locus": "GCA002090215_00190" }, { "type": "cds", "sequence": "contig_1", "start": 108246, "stop": 108569, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATATTAATATTAATATGGGAGAGCAATCATCCAAAAACATGAATTCTTTTGAAAATCATGAATCAAATGAACGAAATAGTCTTCAAATTTATCAACAACATTCATCTATGATGTCAGAAACAAAGAGAAAACAAAGTAGCTTAAGTTATGATGCCAGAATCCTTTTACCTAAAAGTCCCTATAACACAAAGAAAACTTTATTTTTAATTATTTTCTTTTCCATTATCTCCTTGATATTGGCAACTATAGGTAGTGTGTTCATTTCTTTTGCTATAGTTCAAATTTCTATACCTTTTTACGTTATAGGTGGAGTTATTTGA", "aa": "MNININMGEQSSKNMNSFENHESNERNSLQIYQQHSSMMSETKRKQSSLSYDARILLPKSPYNTKKTLFLIIFFSIISLILATIGSVFISFAIVQISIPFYVIGGVI", "aa_hexdigest": "f546d3e2fc5d59c17b13b5b9171c7e6a", "start_type": "ATG", "rbs_motif": "AACAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12154.950799999988, "isoelectric_point": 9.397250175476074 }, "id": "APKECPOBEN_231", "locus": "GCA002090215_00191" }, { "type": "cds", "sequence": "contig_1", "start": 108855, "stop": 109064, "strand": "+", "frame": 3, "gene": null, "product": "putative protein MPN_086", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P75607", "UniRef:UniRef90_P75607" ], "nt": "ATGGAGTCTGCACTAAACCAAGAGTTTCAAATTGATTTTTGTGTAAAAAACAAAAAACTGTTAAAAATTTTAGCTAACGTTCTTATTGCTTCTTGGTTATCGTTTGTTGTTTTTTTAATCCTAGGTTGTATAGCTATTGATCTTTTTCGATTTGATCTTTATTCCCAATTTTTCTTTAATCACTTAAGTACTTTATCTGCTTTAGCATGA", "aa": "MESALNQEFQIDFCVKNKKLLKILANVLIASWLSFVVFLILGCIAIDLFRFDLYSQFFFNHLSTLSALA", "aa_hexdigest": "1343b7ae5fddda2403c543ec02b2302e", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 108855, "stop": 109172, "observations": { "mutation": [ 109062 ], "stop": [ 109062 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75607", "query_cov": 1.0, "subject_cov": 0.6571428571428571, "identity": 1.0, "score": 131.0, "evalue": 1.77e-38, "gene_start": 1, "gene_end": 69, "reference_start": 1, "reference_end": 69, "reference_sequence": "MESALNQEFQIDFCVKNKKLLKILANVLIASWLSFVVFLILGCIAIDLFRFDLYSQFFFNHLSTLSALAWTFFVLAILFGAATLAINGFFYKEIRKKSAFKEDSK" }, "paralog": false, "identity": 0.9904761904761905, "score": 202.0, "evalue": 5.91e-69, "description": "Internal stop codon at 109062. Nonsense mutation around 109062.", "psc": { "uniref90_id": "UniRef90_P75607", "product": "Uncharacterized protein MPN_086", "uniref50_id": "UniRef50_P75607", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75607", "UniRef:UniRef50_P75607", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75607", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75607" ], "product": "Uncharacterized protein MPN_086" } }, "id": "APKECPOBEN_232", "locus": "GCA002090215_00192" }, { "type": "cds", "sequence": "contig_1", "start": 109844, "stop": 110131, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_017532936.1", "SO:0001217", "UniParc:UPI00037BE6F2", "UniRef:UniRef100_UPI00037BE6F2", "UniRef:UniRef50_A0AAV5N6K4", "UniRef:UniRef90_A0AAV5N6K4" ], "nt": "ATGGAAAAAGAAACAAAGAAAGATTTATTAAGTGAAGTGAAAAATAGCTTTCATTCTTCTAACAAAAATATTAAAAGCGAAATTATTTTGAAGAACACTTCAGGAAATAATATTTATTTAAGAAGCGATGCGAGAAGGCTTTTACCTAGAATCCCTTATAACACAAAGAGTGCATTATTATTCACCCTTTTATTTGCATTTCTATCTGGCCTTTCTTATGGTCTTGCATCTTTATGTATTAACTTCTCGTTTACTTCGCCACAAATTTTCATTGTCTTAATTTATTAG", "aa": "MEKETKKDLLSEVKNSFHSSNKNIKSEIILKNTSGNNIYLRSDARRLLPRIPYNTKSALLFTLLFAFLSGLSYGLASLCINFSFTSPQIFIVLIY", "aa_hexdigest": "445ca0b6a49cf5e927a3d698b78760ae", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00037BE6F2", "ncbi_nrp_id": "WP_017532936.1", "uniref100_id": "UniRef100_UPI00037BE6F2", "db_xrefs": [ "SO:0001217", "UniParc:UPI00037BE6F2", "RefSeq:WP_017532936.1", "UniRef:UniRef100_UPI00037BE6F2" ] }, "ips": { "uniref100_id": "UniRef100_UPI00037BE6F2", "uniref90_id": "UniRef90_A0AAV5N6K4" }, "psc": { "uniref90_id": "UniRef90_A0AAV5N6K4", "uniref50_id": "UniRef50_A0AAV5N6K4" }, "pscc": { "uniref50_id": "UniRef50_A0AAV5N6K4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAV5N6K4" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10808.48479999999, "isoelectric_point": 9.721655082702636 }, "id": "APKECPOBEN_233", "locus": "GCA002090215_00193" }, { "type": "cds", "sequence": "contig_1", "start": 110384, "stop": 110839, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_017532935.1", "SO:0001217", "UniParc:UPI0003674DF5", "UniRef:UniRef100_UPI0003674DF5", "UniRef:UniRef50_A0AAX0S4M2", "UniRef:UniRef90_A0AAX0S4M2" ], "nt": "GTGAAACAGAATTTAAGTAGTAGAAACTTTATTAAAAAGTCAGAAAAAACAGTATCAACTACTGTAAATATAAATACAGAAGCTAGTAGTTATATTGCCAAAAATCTAGAAAACCAAAAGGCAGTTCTTTCAGCAAAGGAATTAGAAAAACAAGAGCTTTTACTAGTGCGCTTAAATGCAGTAAAAAGATTTCCGTGCTATCCATACAATACAAAAAAGGCATTTAATATTTCTGTTTTATTTGTTCTATTAGCTGGTCTATTTTTTACTGCGTCTTATTTCTCTATTTTTTATTCGATTTTTACTAGGTTTAAAGCTTTACTTATTTTCTTAGGAGTAGTTTTTATTCTTATAACTTTCATTATGCTAATTCATTGCTATATATGGTCAGTAAGGTTCAAATCAGAGTACCTTGCTAATCGTATTTATGCACTGAATTTTCAAAAAGGAGCCTAA", "aa": "MKQNLSSRNFIKKSEKTVSTTVNINTEASSYIAKNLENQKAVLSAKELEKQELLLVRLNAVKRFPCYPYNTKKAFNISVLFVLLAGLFFTASYFSIFYSIFTRFKALLIFLGVVFILITFIMLIHCYIWSVRFKSEYLANRIYALNFQKGA", "aa_hexdigest": "6734e87331b99e2353ef99ce193c0142", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0003674DF5", "ncbi_nrp_id": "WP_017532935.1", "uniref100_id": "UniRef100_UPI0003674DF5", "db_xrefs": [ "SO:0001217", "UniParc:UPI0003674DF5", "RefSeq:WP_017532935.1", "UniRef:UniRef100_UPI0003674DF5" ] }, "ips": { "uniref100_id": "UniRef100_UPI0003674DF5", "uniref90_id": "UniRef90_A0AAX0S4M2" }, "psc": { "uniref90_id": "UniRef90_A0AAX0S4M2", "uniref50_id": "UniRef50_A0AAX0S4M2" }, "pscc": { "uniref50_id": "UniRef50_A0AAX0S4M2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAX0S4M2" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17497.51309999999, "isoelectric_point": 9.933433723449706 }, "id": "APKECPOBEN_234", "locus": "GCA002090215_00194" }, { "type": "cds", "sequence": "contig_1", "start": 110889, "stop": 111116, "strand": "+", "frame": 3, "gene": null, "product": "putative protein MPN_088", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD64A6", "UniRef:UniRef100_P75605", "UniRef:UniRef50_P75605", "UniRef:UniRef90_P75605" ], "nt": "ATGATAGAAATAAATAATTTGACCGCTTTAGAAAGGGATGAAAAAGTAGAAACGGAAGACATTAATACCAATGTAATAGCAAATACTTATACAGTGCAAGCCAATGTTCCAACTGCATTTCAAACCAGTGAAACAAATAGTAATACTGTTACTAACGTCCCAAAATCTCCAGAGTTAATAAGTATTCAAAGATTAGAAAGATTAAAAAATAGGTACACTTTCCTATAA", "aa": "MIEINNLTALERDEKVETEDINTNVIANTYTVQANVPTAFQTSETNSNTVTNVPKSPELISIQRLERLKNRYTFL", "aa_hexdigest": "63509dd9769b595e72105a39ef4b8b97", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD64A6", "uniref100_id": "UniRef100_P75605", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD64A6", "UniRef:UniRef100_P75605" ] }, "ips": { "uniref100_id": "UniRef100_P75605", "uniref90_id": "UniRef90_P75605" }, "psc": { "uniref90_id": "UniRef90_P75605", "product": "Uncharacterized protein MPN_088", "uniref50_id": "UniRef50_P75605" }, "pscc": { "uniref50_id": "UniRef50_P75605", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75605" ], "product": "Uncharacterized protein MPN_088" }, "genes": [], "id": "APKECPOBEN_235", "locus": "GCA002090215_00195" }, { "type": "cds", "sequence": "contig_1", "start": 111476, "stop": 111655, "strand": "+", "frame": 2, "gene": null, "product": "restriction endonuclease subunit S", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0034DD5284", "UniRef:UniRef90_UPI0019169453" ], "nt": "GTGGAGATTAAAACTTACAAAATTAAAGATATTTGTGAAGCTTCACATGGAAGAGAAATAAATACTAAATATCTCCGTGAAAATCAAGGAATTTATCCTGTTTATTCATCAGCAACTTCTAATGAAGGTGAAATGGGCAGAATCAAAACTTACGATTTTGATGGTGAATATGTTACCTGA", "aa": "MEIKTYKIKDICEASHGREINTKYLRENQGIYPVYSSATSNEGEMGRIKTYDFDGEYVT", "aa_hexdigest": "fad4a179cb312d5d5e4ee0fcbc69cd9b", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 111476, "stop": 111712, "observations": { "mutation": [ 111653, 111665 ], "stop": [ 111653, 111665 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0019169453", "query_cov": 1.0, "subject_cov": 0.7468354430379747, "identity": 1.0, "score": 127.0, "evalue": 3.64e-37, "gene_start": 1, "gene_end": 59, "reference_start": 1, "reference_end": 59, "reference_sequence": "MEIKTYKIKDICEASHGREINTKYLRENQGIYPVYSSATSNEGEMGRIKTYDFDGEYVTWTTRWSYAGSIYYRNGKFSG" }, "paralog": false, "identity": 0.9615384615384616, "score": 155.0, "evalue": 1.12e-50, "description": "Internal stop codon at 111653, 111665. Nonsense mutation around 111653, 111665.", "psc": { "uniref90_id": "UniRef90_UPI0019169453", "product": "restriction endonuclease subunit S", "uniref50_id": "UniRef50_UPI0034DD5284", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0019169453", "UniRef:UniRef50_UPI0034DD5284" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0034DD5284", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0034DD5284" ], "product": "restriction endonuclease subunit S" } }, "id": "APKECPOBEN_236", "locus": "GCA002090215_00196" }, { "type": "cds", "sequence": "contig_1", "start": 111636, "stop": 111734, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTGAATATGTTACCTGAACTACACGTTGAAGTTATGCCGGTAGCATTTACTATCGAAATGGTAAATTTAGTGCTTCCTCAAATTGTGGAATTTTAA", "aa": "MVNMLPELHVEVMPVAFTIEMVNLVLPQIVEF", "aa_hexdigest": "dc9090817d4032673e720697307a0d38", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3683.4665999999997, "isoelectric_point": 4.087655830383301 }, "id": "APKECPOBEN_237", "locus": "GCA002090215_00197" }, { "type": "cds", "sequence": "contig_1", "start": 111875, "stop": 112021, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAAATTCAAGAAAAAATTGCTACTATCTTAGATACTTTTACCGAGCTAAGCGCCGAGCTAAGCGCCGAGCTAAGCGCCGAGCTAAGAGAGCGAAAGAAGCAGTATGCCTTCTATCGCGATTATTTACTTAACCTAAAAAATTGA", "aa": "MQIQEKIATILDTFTELSAELSAELSAELRERKKQYAFYRDYLLNLKN", "aa_hexdigest": "073bbce6ce3c1cd9bd816e9b8b758e83", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5696.4431, "isoelectric_point": 5.221025276184081 }, "id": "APKECPOBEN_238", "locus": "GCA002090215_00198" }, { "type": "cds", "sequence": "contig_1", "start": 112166, "stop": 112558, "strand": "+", "frame": 2, "gene": null, "product": "Putative type I specificity subunit S-MpnORF289P C-terminus", "db_xrefs": [ "EC:3.1.21.3", "GO:0003677", "GO:0009307", "KEGG:K01154", "SO:0001217", "UniRef:UniRef50_P75487", "UniRef:UniRef90_P75487" ], "nt": "TTGGTTTACAGTAAAACTTTTCGAGTTGAAGAAAAATCCATTACGGTAAGTGCTAGAGGCACAATTGGTGCCGTTTTCTATCGTGATTTTGCTTATTTGCCTGCTGTTAGTTTAATTTGCTTTGTACCTAAAGAAGAATTTGATATAAGGTTTTTATTCCACGCATTAAGAGCCATTAAATTTAAAAAGCAAGGTTCTGCAACAGGCCAGTTAACAGTGGCACAATTTAAGGAGTATGGTATTCATGTACCTTCGCTGAAAAAACAGAAAGAAATCGCTGCTATCTTAGATCCACTTTACAGCTTTTTTACTGATCTAAACGAAGGTATTCCTGCTGAAATTGAACTGCGCAAAAAGCAATTGGATTACTATCAGAACTTCTTATTTAATTGA", "aa": "MVYSKTFRVEEKSITVSARGTIGAVFYRDFAYLPAVSLICFVPKEEFDIRFLFHALRAIKFKKQGSATGQLTVAQFKEYGIHVPSLKKQKEIAAILDPLYSFFTDLNEGIPAEIELRKKQLDYYQNFLFN", "aa_hexdigest": "36f83b9b9a34b5f0b3a05becf256ee23", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75487", "query_cov": 1.0, "subject_cov": 0.896551724137931, "identity": 0.985, "score": 256.0, "evalue": 2.23e-86, "valid": true, "product": "Putative type I specificity subunit S.MpnORF289P C-terminus", "ec_ids": [ "3.1.21.3" ], "uniref50_id": "UniRef50_P75487", "kegg_orthology_id": "K01154", "go_ids": [ "GO:0003677", "GO:0009307" ] }, "pscc": { "uniref50_id": "UniRef50_P75487", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75487" ], "product": "Putative type I specificity subunit S.MpnORF289P C-terminus" }, "genes": [], "id": "APKECPOBEN_239", "locus": "GCA002090215_00199" }, { "type": "cds", "sequence": "contig_1", "start": 113721, "stop": 113843, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W7C9", "UniRef:UniRef90_A0AB38W7C9" ], "nt": "ATGGGCGGCGCGCACAACATCCAGCCCCCCATTGAGCGGTGTCGTAAAGGGCGTGTAGGGGGTGAGGGTGTTGTGGTCTTGGAGGAGGTAGGTGTTGGAAACTATTTATTAATCTTTAAGTAA", "aa": "MGGAHNIQPPIERCRKGRVGGEGVVVLEEVGVGNYLLIFK", "aa_hexdigest": "f7368d3308c1261e8c6603226c024eca", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0AB38W7C9", "query_cov": 0.925, "subject_cov": 0.925, "identity": 0.919, "score": 73.2, "evalue": 1.43e-16, "valid": true, "uniref50_id": "UniRef50_A0AB38W7C9" }, "pscc": { "uniref50_id": "UniRef50_A0AB38W7C9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W7C9" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4292.982899999999, "isoelectric_point": 7.9588960647583 }, "id": "APKECPOBEN_240", "locus": "GCA002090215_00200" }, { "type": "cds", "sequence": "contig_1", "start": 113877, "stop": 114335, "strand": "+", "frame": 3, "gene": null, "product": "Putative MgpC-like protein MPN_366", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75415", "UniRef:UniRef90_A0AB38W808" ], "nt": "GTGGGACAGAACAAGTTGGTGGCGCTGGTCCGCTCAGCGTTGGTCAGGGTGAAGTTTCAAGATAGTTCGAGCCAACAAGGCACTGAGAGTAATGGCCAAGACCAAAATGCCCTTAGTTTTGATACCACCAAGTCCCAACAAGCCTTACAGCAAGCTGGCGATCAATCAGGAACTTCATCTAATTCCACCCAAGAAGACTTCGCCAGCTACATCCTCATCTTTAAAGCCGCGCCCAGGGCCACGTGGGTGTTTGAACGCAAGATTAAGTTGGCGTTGCCCTACGTTAAGCAGGAAAGTCAGGGTTCCGACGATCAAGGTTCCAATGGTAAGGGCTCCCTCTACACCACCTTAAAAGACCTCCTCGTCGAACAACCCGTGACCCCTTACACGCCCAATGCGGGGTTAGCCCGGGTGAATGGGGTTGCTCAGGACAAGGTGCATGGGTTGGAGTCTGGGTGA", "aa": "MGQNKLVALVRSALVRVKFQDSSSQQGTESNGQDQNALSFDTTKSQQALQQAGDQSGTSSNSTQEDFASYILIFKAAPRATWVFERKIKLALPYVKQESQGSDDQGSNGKGSLYTTLKDLLVEQPVTPYTPNAGLARVNGVAQDKVHGLESG", "aa_hexdigest": "624c797a5e6239e80827d7a7c87242db", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 113829, "stop": 114542, "observations": { "mutation": [ 113871, 114333, 114441 ], "stop": [ 113871, 114333, 114441 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB38W808", "query_cov": 1.0, "subject_cov": 0.6413502109704642, "identity": 0.993, "score": 288.0, "evalue": 2.83e-97, "gene_start": 1, "gene_end": 152, "reference_start": 17, "reference_end": 168, "reference_sequence": "MRAAHLHTSYELVDWKVGQNKLVALVRSALVRVKFQDSSSQQGTESNGQDQNALSFDTTKSQQALQQAGDQSGTSSNSTQEDFASYILIFKAAPRATWVFERKIKLALPYVKQESQGSDDQGSNGKGSLYTTLKDLLVEQPVTPYTPNAGLARVNGVAQDKVHGLESGWDNQRSQKNLTNNPGPKAVTGFKLDKGRAYRKLNESWPVSNPSIRPRLARGKVPQNEKRRRQMLRVMLP" }, "paralog": false, "identity": 0.9789029535864979, "score": 453.0, "evalue": 1.85e-164, "description": "Internal stop codon at 113871, 114333, 114441. Nonsense mutation around 113871, 114333, 114441.", "psc": { "uniref90_id": "UniRef90_A0AB38W808", "uniref50_id": "UniRef50_P75415", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB38W808", "UniRef:UniRef50_P75415" ] }, "pscc": { "uniref50_id": "UniRef50_P75415", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75415" ], "product": "Putative MgpC-like protein MPN_366" } }, "id": "APKECPOBEN_241", "locus": "GCA002090215_00201" }, { "type": "cds", "sequence": "contig_1", "start": 114546, "stop": 114755, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTCAGCAAGTGGGGGGCTGGAAGTGCTTTTGGTTTACAAGGCAATGGCTCGAACAGTTCGGGGTTAAGACCGCTCTTGAAGCGCACCGCCCAAATTAATTTAAGGCAAACCCAAGACAACGCGCAAACTGGTAAATTCTCCAAATACCTCAACACCGCCCAGGCCTTGCACCAGATGGGGGTGATAGTGCCATCTTTGGAAACGTGA", "aa": "MVSKWGAGSAFGLQGNGSNSSGLRPLLKRTAQINLRQTQDNAQTGKFSKYLNTAQALHQMGVIVPSLET", "aa_hexdigest": "a0f96b7c5c03c3c6366f8b2f34c2a0d5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7375.280200000003, "isoelectric_point": 10.54859104156494 }, "id": "APKECPOBEN_242", "locus": "GCA002090215_00202" }, { "type": "cds", "sequence": "contig_1", "start": 114787, "stop": 114996, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF05220.18" ], "nt": "GTGCTGTTGGTGGTGTTTCAGTCCAAGCAGCGACTCGGCTCGACTTCTACAAATGAAGATCTGCCGAATGTAATAACAAAGTTATACCACACCTCCACGTCCCAACTCGCTTACTTAAATGGCCAGATCGTTGTGATGGGTTCAAACGCGGTACCGAGTTTGTGGTATTGGGTTGTCGGGGAGGACCAGGAATCGGGTCGCGCAACCTGA", "aa": "MLLVVFQSKQRLGSTSTNEDLPNVITKLYHTSTSQLAYLNGQIVVMGSNAVPSLWYWVVGEDQESGRAT", "aa_hexdigest": "0f81baddb538e84c88bdd5db7f3b2824", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF05220.18", "name": "MgpC", "length": 24, "aa_cov": 0.34782608695652173, "hmm_cov": 1.0, "evalue": 8.400425159367912e-11, "score": 37.082950592041016, "start": 46, "stop": 69 } ], "seq_stats": { "molecular_weight": 7630.5354000000025, "isoelectric_point": 5.53852787017822 }, "id": "APKECPOBEN_243", "locus": "GCA002090215_00203" }, { "type": "cds", "sequence": "contig_1", "start": 115057, "stop": 115695, "strand": "+", "frame": 1, "gene": "mgpC", "product": "MgpC family cytadherence protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002246AD21", "UniRef:UniRef90_UPI002246AD21" ], "nt": "TTGGGTTTTAAAGATGACTCCAATTCGGATTCCAAGAATTCGAATTTGAAGGCTCAAGGCCTCACCCAACCCGCCTACCTCATCGCCGGTCTTGACGTTGTGGCCGACCACCTCGTCTTTGCGGCCTTTAAAGCGGGCGCGGTGGGGTATGATATGAGCACGGAAAACAGTGCTGCCACCAAAGACCAAGCACTCGCCTGGTCGACCACGGCCGGGTTGGACAGTGCTGGGGGGTACAAGGCCTTGGTGGAAAACACGGCCGGGCTCAACGGTCCGATTAATGGCTTGTTTACCCTGCTCGACAGCTTTGCCTATGTGACCCCGGTGAGTGGCATGAAAGGGGGTAGTCAGAATAACGAAGAAGTGCAGACCAAGTATCCCGTTAAGGATGATAGTAAGGCTTCCGCCAAAATTGCGTCCTTAATTAATGCCAGCCCACTCAACAGTTATGGGGATGATGGCGTGACCGTGTTTGATGCCCTGGGCCTTAACTTTAACTTTAAGTTGAACGAGGCGCGCTTGCCGTCGCGCACCGACCAACTGCTTGTGTATGGGATTGTAAACGAAAGTGAACTGAAGTCCGCGCGCGAAAATGCCCAATCAACTTCCGACGCTAATTCGAACACCAAAGTCAAGTGA", "aa": "MGFKDDSNSDSKNSNLKAQGLTQPAYLIAGLDVVADHLVFAAFKAGAVGYDMSTENSAATKDQALAWSTTAGLDSAGGYKALVENTAGLNGPINGLFTLLDSFAYVTPVSGMKGGSQNNEEVQTKYPVKDDSKASAKIASLINASPLNSYGDDGVTVFDALGLNFNFKLNEARLPSRTDQLLVYGIVNESELKSARENAQSTSDANSNTKVK", "aa_hexdigest": "0fdbbcb3243d47a65162a7f6fe171f2e", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002246AD21", "query_cov": 0.9292452830188679, "subject_cov": 0.8565217391304348, "identity": 0.924, "score": 345.0, "evalue": 7.26e-119, "valid": true, "product": "MgpC family cytadherence protein", "uniref50_id": "UniRef50_UPI002246AD21" }, "pscc": { "uniref50_id": "UniRef50_UPI002246AD21", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002246AD21" ], "product": "MgpC family cytadherence protein" }, "genes": [ "mgpC" ], "id": "APKECPOBEN_244", "locus": "GCA002090215_00204" }, { "type": "cds", "sequence": "contig_1", "start": 116429, "stop": 116683, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01519.22" ], "nt": "GTGAACACAGAATACGTAACGCGTAAAGAGTTTAATGAGTACAAAGACTCAAACGACCAACGACTTACAAAGATTGAAACCACTTTGGCCGCCCAAGGTGAACAAATAAGAATCCAGGGTGAGCAAATTAAAGAACTTCAAATCGAGCAAAAAGCTCAGGGAGAAACCTTAAAGCTAATCTTGCAAACGCTGCAAAAGATGAGCGATCGTTTGAACAAGATGGATGTTCGTCTAGATAAACTAGAGTCTAAGTAG", "aa": "MNTEYVTRKEFNEYKDSNDQRLTKIETTLAAQGEQIRIQGEQIKELQIEQKAQGETLKLILQTLQKMSDRLNKMDVRLDKLESK", "aa_hexdigest": "d94ccaf869995906d56060fb4b90062d", "start_type": "GTG", "rbs_motif": "AACAA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01519.22", "name": "DUF16", "length": 88, "aa_cov": 0.9642857142857143, "hmm_cov": 1.0, "evalue": 8.371705652180985e-27, "score": 89.21607208251953, "start": 2, "stop": 82 } ], "seq_stats": { "molecular_weight": 9914.184799999994, "isoelectric_point": 6.300397682189941 }, "id": "APKECPOBEN_245", "locus": "GCA002090215_00205" }, { "type": "cds", "sequence": "contig_1", "start": 116891, "stop": 117139, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0H3DJX7", "UniRef:UniRef90_A0A0H3DJX7" ], "nt": "TTGAAAGTTTTATTTACTTTCCAAACCTTACAAAGAAAGGAGGATTTAAACATGAAAAAAGTTAAAGCATTAAGGAATCTTTTTCTAGAAAAAGCCGAAGAAAAGCTTAAATACCCCAAAATTGCTTCAATGGTAGCTTACTTTGATCCAAAGGAAGATGGTACTTGTGAATTCATTATGAAAATTAAAACTGTCAATGAAGTTACTGATAACGAAGTAAAAAGCGAGGAAAAGCCTTTAGGCACTTGA", "aa": "MKVLFTFQTLQRKEDLNMKKVKALRNLFLEKAEEKLKYPKIASMVAYFDPKEDGTCEFIMKIKTVNEVTDNEVKSEEKPLGT", "aa_hexdigest": "bf2e33f63321979240f6528f7b20ff2f", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A0H3DJX7", "query_cov": 1.0, "subject_cov": 0.9647058823529412, "identity": 1.0, "score": 156.0, "evalue": 2.89e-48, "valid": true, "uniref50_id": "UniRef50_A0A0H3DJX7" }, "pscc": { "uniref50_id": "UniRef50_A0A0H3DJX7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0H3DJX7" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9589.156999999996, "isoelectric_point": 8.663857841491701 }, "id": "APKECPOBEN_246", "locus": "GCA002090215_00206" }, { "type": "cds", "sequence": "contig_1", "start": 117201, "stop": 117503, "strand": "+", "frame": 3, "gene": null, "product": "Adhesin P1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75267", "UniRef:UniRef90_A0A0H3DN54" ], "nt": "TTGCTGTTGGTATTTTTATTCTCCCCCACGCTTTCCCGTTTCTCCACCGGGTTCAACCTCGCGGGGACTGTTTTGGACCAGGTGTTGGATTATGTTCCCTGGATTGGGAATGGGTACAGGTATGGGAATGACCACCGGGGGAGCAACAGTAGTACGAGTGGGGTAACGACCCAGGGACAATCCCAAAATGCATCCAGTAACGAACCCGCACCGACGTTTTCGAATGTCGGCGTTGGTCTCAAAGCCAACGTCCAAGCGACGCTCGGGGGCAGTCAGACGACGACTACCAAGGGTTCGCCTTGA", "aa": "MLLVFLFSPTLSRFSTGFNLAGTVLDQVLDYVPWIGNGYRYGNDHRGSNSSTSGVTTQGQSQNASSNEPAPTFSNVGVGLKANVQATLGGSQTTTTKGSP", "aa_hexdigest": "47290fd7a38e5beb455e8da216e2716a", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 117201, "stop": 117806, "observations": { "mutation": [ 117501, 117534, 117549, 117798 ], "stop": [ 117501, 117534, 117549, 117798 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A0H3DN54", "query_cov": 1.0, "subject_cov": 0.4291845493562232, "identity": 1.0, "score": 192.0, "evalue": 3.05e-60, "gene_start": 1, "gene_end": 100, "reference_start": 1, "reference_end": 100, "reference_sequence": "MLLVFLFSPTLSRFSTGFNLAGTVLDQVLDYVPWIGNGYRYGNDHRGSNSSTSGVTTQGQSQNASSNEPAPTFSNVGVGLKANVQATLGGSQTTTTKGSPWRTLDPANLQLWTGAGWRNDKASNKQSDENHTTFKSATGMGQQGQSGTSAGNPDALKQDKITKENSGNLKTEDGTATNSTTAFASNYTNLPPTADWPNALSFTNKNNAQRAQLFLRGLLGSIPVLVNKSGENN" }, "paralog": false, "identity": 0.945273631840796, "score": 373.0, "evalue": 9.97e-134, "description": "Internal stop codon at 117501, 117534, 117549, 117798. Nonsense mutation around 117501, 117534, 117549, 117798.", "psc": { "uniref90_id": "UniRef90_A0A0H3DN54", "product": "Adhesin P1", "uniref50_id": "UniRef50_P75267", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A0H3DN54", "UniRef:UniRef50_P75267" ] }, "pscc": { "uniref50_id": "UniRef50_P75267", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75267" ], "product": "Putative adhesin P1-like protein MPN_131" } }, "id": "APKECPOBEN_247", "locus": "GCA002090215_00207" }, { "type": "cds", "sequence": "contig_1", "start": 117618, "stop": 117800, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGCAAGCAGGGACAATCAGGTACCTCCGCGGGGAATCCCGATGCGTTAAAGCAGGATAAAATCACCAAGGAAAACTCGGGCAACCTCAAAACGGAGGATGGTACTGCTACTAATTCGACGACGGCATTCGCGTCGAATTACACCAACCTCCCCCCCAACCTCACCCCCACCGCTGATTGA", "aa": "MGKQGQSGTSAGNPDALKQDKITKENSGNLKTEDGTATNSTTAFASNYTNLPPNLTPTAD", "aa_hexdigest": "fcd792a27bc05112bcbd73637a889dcf", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6171.552700000004, "isoelectric_point": 5.047041130065917 }, "id": "APKECPOBEN_248", "locus": "GCA002090215_00208" }, { "type": "rRNA", "sequence": "contig_1", "start": 118288, "stop": 119803, "strand": "+", "gene": "rrs", "product": "16S ribosomal RNA", "coverage": 0.9889106327462491, "score": 1440.1, "evalue": 3.2e-44, "db_xrefs": [ "GO:0005840", "GO:0003735", "RFAM:RF00177", "KEGG:K01977", "SO:0001000" ], "nt": "TTTTCTGAGAGTTTGATCCTGGCTCAGGATTAACGCTGGCGGCATGCCTAATACATGCAAGTCGATCGGAAGTAGTAATACTTTAGAGGCGAACGGGTGAGTAACACGTATCCAATCTACCTTATAATGGGGGATAACTAGTTGAAAGACTAGCTAATACCGCATAAGAACTTTGGTTCGCATGAATCAAAGTTGAAAGGACCTGCAAGGGTTCGTTATTTGATGAGGGTGCGCCATATCAGCTAGTTGGTGGGGTAACGGCCTACCAAGGCAATGACGTGTAGCTATGCTGAGAAGTAGAATAGCCACAATGGGACTGAGACACGGCCCATACTCCTACGGGAGGCAGCAGTAGGGAATTTTTCACAATGAGCGAAAGCTTGATGGAGCAATGCCGCGTGAACGATGAAGGTCTTTAAGATTGTAAAGTTCTTTTATTTGGGAAGAATGACTTTAGCAGGTAATGGCTAGAGTTTGACTGTACCATTTTGAATAAGTGACGACTAACTATGTGCCAGCAGTCGCGGTAATACATAGGTCGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCAAGCGCAGGCGGATTGAAAAGTCTGGTGTTAAAGGCAGCTGCTTAACAGTTGTATGCATTGGAAACTATTAATCTAGAGTGTGGTAGGGAGTTTTGGAATTTCATGTGGAGCGGTGAAATGCGTAGATATATGAAGGAACACCAGTGGCGAAGGCGAAAACTTAGGCCATTACTGACGCTTAGGCTTGAAAGTGTGGGGAGCAAATAGGATTAGATACCCTAGTAGTCCACACCGTAAACGATAGATACTAGCTGTCGGGGCGATCCCCTCGGTAGTGAAGTTAACACATTAAGTATCTCGCCTGGGTAGTACATTCGCAAGAATGAAACTCAAACGGAATTGACGGGGACCCGCACAAGTGGTGGAGCATGTTGCTTAATTCGACGGTACACGAAAAACCTTACCTAGACTTGACATCCTTGGCAAAGTTATGGAAACATAATGGAGGTTAACCGAGTGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCGTTAGTTACATTGTCTAGCGAGACTGCTAATGCAAATTGGAGGAAGGAAGGGATGACGTCATATCATCATGCCCCTTATGTCTAGGGCTGCAAACGTGCTACAATGGCCAATACAAACAGTCGCCAGCTTGTAAAAGTGAGCAAATCTGTAAAGTTGGTCTCAGTTCGGATTGAGGGCTGCAATTCGTCCTCATGAAGTCGGAATCACTAGTAATCGCGAATCAGCTATGTCGCGGTGAATACGTTCTCGGGTCTTGTACACACCGCCCGTCAAACTATGAAAGCTGGTAATATTTAAAAACGTGTTGCTAACCATTAGGAAGCGCATGTCAAGGATAGCACCGGTGATTGGAGTTAAGTCGTAACAAGGTACCCCTACGAGAACGTGGGGGTGGATCACCTCCTTT", "id": "APKECPOBEN_39", "locus": "GCA002090215_00209" }, { "type": "rRNA", "sequence": "contig_1", "start": 120031, "stop": 122935, "strand": "+", "gene": "rrl", "product": "23S ribosomal RNA", "coverage": 0.9931623931623932, "score": 2687.1, "evalue": 8.3e-06, "db_xrefs": [ "GO:0005840", "GO:0003735", "RFAM:RF02541", "KEGG:K01980", "SO:0001001" ], "nt": "CAATAAGTTACTAAGGGCTTATGGTGGATGCCTTGGCACTAATAGGCGATGAAGGACGTGTTAACCTGCGATAAGCTTCGGGTAGGTGGTAAGAACCTCAGATCCGGAGATTTCCGAATGGAGCAATCCGGTAGTTGGAAACAGCTATCATTAATTGATGAATAAATAGTCAATTAAAGCAATACGTGGTGAAGTGAAACATCTCAGTAGCCACAGGAAAAGAAAACGAATGTGATTCCGTGTGTAGTGGCGAGCGAAAGCGGAACAGGCCAAACTTATCATTAGATAGGGGTTGTAGGGCTTGCAATGTGGACTTGAAAACGATAGAAGAAGCTGTTGGAAAGCAGCGCGCAAAAGGGTGATAGCCCCGTATTTGAAATTGTTTTCATACCTAGCGAGATCCCTGAGTAGCTCGGAAAACGTTATTTTGAGTGAATCTGCCCAGACCATTGGGTAAGCCTAAATACTAATTAGTGACCGATAGCGAAACAGTACCGTGAGGGAAAGGTGAAAAGAACCCAGAGATGGGAGTGAAATAGATTCTGAAACCATATGCCTACAACGTGTCAGAGCACATTAATGTGTGATGGCGTGCGTTTTGAAGTATGAGCCGGCGAGTTATGATAGCAAGCGTTAGTTAACCAGGAGATGGGGAGCTGTAGCGAAAGCGAGTTTTAAAAGAGCGTTTGTTTGTTATTATAGACCCGAAACGGGTTGAGCTAGTCATGAGCAGGTTGAAGGTTGAGTAACATCAACTGGAGGACCGAACCGACTCTCGTTGAAACGATAGCGGATGACTTGTGATTAGGGGTGAAATTCCAATCGAAATCCGTGATAGCTGGTTCTCGTCGAAATAGCTTTAAGGCTAGCGTGAGATCACAAATAAGTGGAGGTAAAGCTACTGAATGTATGATGGCGCCACCTAGGCGTACTGAATACAATTAAACTCTGAATGCCATTTATTTTATTCTCGCAGTCAGACAGTGGGGGATAAGCTTCATTGTCAAGAGGGGAAGAGCCCAGATCATTAAATAAGGTCCCCAAAATATACTAAGTGGAAAAGGATGTGAAAGTGCTAAAACAGCAAGGATGTTGGCTTAGAAGCAGCCATCGTTTAAAGAGTGCGTAACAGCTCACTTGTCGAGTGTTTTTGCGCCGAAGATGTAACGGGGCTAAGTATATTACCGAATTTATGGATAAGATTTATATCTTGTGGTAGACGAGCGTTGTATTGGAGTTGAAGTCAAAGCGTGAGCATTGGTGGATCCAATACAAGTGAGAATGCCGGCGTGAGTAACGCTTGGGAGTGAGAATCTCCCAAACCGATTGACTAAGGTTTCCTGGACCAGGGTCGTCCTTCCAGGGTTAGTCTGGACCTAAGCTGAGGCTGAAAAGCGTAGGCGATGGACAACAGGTTAATATTCCTGTACTTACAGTTAGACTGATGGAGTGACAAAGAAGGTTTTCCACCCCCATAATTGGATTTGGGGATAAATCATAAGGTGGTACAATAGGCAAATCCGTTGTGCATAACATTGAGTGATGATGTCGAGTGAATGAGTGATCAAGTAGCGAAGGTGGTATTAATCATGCTTTCAAGAAAAGCTTCTAGGGTTAATCTAGCTGTAACCAGTACCGAGAACGAACACACGTAGTCAAGGAGAGGATCCTAAGGTTAGCGAGTGAACTATAGCCAAGGAACTCTGCAAATTAACCCCGTAAGTTAGCGAGAAGGGGTGCTTATGTAAAAGTAAGCCGCAGTGAAGAACGAGGGGGGACTGTTTAACTAAAACACAACTCTATGCCAAACCGTAAGGTGATGTATATGGGGTGACACCTGCCCAGTGCTGGAAGGTTAAAGAAGGAGGTTAGCGCAAGCGAAGCTTTTAACTGAAGCCCCAGTGAACGGCGGCCGTAACTATAACGGTCCTAAGGTAGCGAAATTCCTAGTCGGGTAAATTCCGTCCCGCTTGAATGGTGTAACCATCTCTTGACTGTCTCGGCTATAGACTCGGTGAAATCCAGGTACGGGTGAAGACACCCGTTAGGCGCAACGGGACGGGAAGACCCCGTGAAGCTTTACTGTAGCTTAATATTGATCAGGACATTATCATGTAGAGAATAGGTAGGAGCAATCGATGCAAGTTCGCTAGGACTTGTTGATGCGAAAGGTGGAATACTACCCTTGGTTGTGTGCTGTTCTAATTGGTAACTGTTATCCAGTTTCAAGACAGTGTTAGGTGGGCAGTTTGACTGGGGCGGTCGCCTCCTAAAAGGTAACGGAGGCGTACAAAGGTACCTTCAGTACGGTTGGAAATCGTATGTAGAGTGTAATGGTGTAAGGGTGCTTGACTGTGAGACATACAGGTCGAACAGGTGAGAAATCAGGTCATAGTGATCCGGTGGTCCAGTATGGAATGGCCATCGCTCAACGGATAAAAGCTACTCCGGGGATAACAGGCTGATACTGCCCAAGAGTTCATATCGACGGCAGTGTTTGGCACCTCGATGTCGACTCATCTCATCCTCGAGCTGAAGCAGGTTCGAAGGGTTCGGCTGTTCGCCGATTAAAGAGATACGTGAGTTGGGTTCAAACCGTCGTGAGACAGGTTGGTCCCTATCTATTGTGCCCGTAGGAAGATTGAAGAGTGTTGCTTCTAGTACGAGAGGACCGAAGCGAGGACACCTCTTATGCTCCAGTTGTAGCGCCAGCTGCACCGCTGGGTAGTAACGTGTCTATTAGATAAACGCTGAAAGCATCTAAGTGTGAAACTATCTCAAAGATTAATCTTCCCATTTCGCAAGAAAGTAAGAGCCGTCAAAGACGATGACGTTGATAGGTTACAGGTGTAAGCATAGTGATATGTTGAGCTGAGTAATACTAATTGCTCGAGGACTTATTGGA", "id": "APKECPOBEN_41", "locus": "GCA002090215_00210" }, { "type": "rRNA", "sequence": "contig_1", "start": 122973, "stop": 123080, "strand": "+", "gene": "rrf", "product": "5S ribosomal RNA", "coverage": 0.907563025210084, "score": 71.9, "evalue": 1.1e-11, "db_xrefs": [ "GO:0005840", "GO:0003735", "RFAM:RF00001", "KEGG:K01985", "SO:0000652" ], "nt": "TTTGGTGCCCATGTCGCTGTGGAAACACCTGGTTCCATTTCGAACCCAGCAGTTAAGCACAGTGGAGCCGAATGTAGCTGTTTCAGTGAGAATAGGAAAGCACCAAGC", "id": "APKECPOBEN_40", "locus": "GCA002090215_00211" }, { "type": "cds", "sequence": "contig_1", "start": 123264, "stop": 123530, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACCAACAATTAAATACCACCAGGAAAAGTACAGCTGCTAGGGGCAGAATGGGACTTGTTGGTGGTATTTTACTCGTCATTGGTACCTGCATAGGGGCAGGTATTTTCTTTAAGAGTGAACGGGTGTTGCAAAACATGGGCGGAAACACCACTTTAGCCTTACTGGTGTGGTTAATGGCAGGGATTACCGTGATCTTAATGGGGTTGGCGCTGGTTGAAATTACTGCTAAAGCAGCCTTTGATGACTTGGCATTATTGTCATGA", "aa": "MNQQLNTTRKSTAARGRMGLVGGILLVIGTCIGAGIFFKSERVLQNMGGNTTLALLVWLMAGITVILMGLALVEITAKAAFDDLALLS", "aa_hexdigest": "1d33371caa2484679fb01873bd16e884", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9235.989599999992, "isoelectric_point": 9.775937461853026 }, "id": "APKECPOBEN_249", "locus": "GCA002090215_00212" }, { "type": "cds", "sequence": "contig_1", "start": 123846, "stop": 124154, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01490.24" ], "nt": "TTGGGTGCAAGTGCTGAACAAATGGAACGCCAACCAGTGGTTCCAGTCAAAGATTTTACTGCCATTTCACCCTTCTTTGGTTGGTTTAGTGCTATGGGCGCGATTTTCTTTGCTTTTGATGGTTTTTACGTGTCAGCAGCAGCGAAAACGCAGTTGAAAAAACCAAAAAAATTACCGCAAGTAATGTTGTTGGGCTTAGGGATTGTGGTACTGATTTACAGCTTAATTGCACTAAGTGTGAGTTTAACCACACCTAACGGTGCCTTTTCGGGACTTGGTGATTGGTTAAAGCACAAAAAGCTTGGTTGA", "aa": "MGASAEQMERQPVVPVKDFTAISPFFGWFSAMGAIFFAFDGFYVSAAAKTQLKKPKKLPQVMLLGLGIVVLIYSLIALSVSLTTPNGAFSGLGDWLKHKKLG", "aa_hexdigest": "467b62b127923dfcb6ac886499fe0257", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01490.24", "name": "Aa_trans", "length": 70, "aa_cov": 0.6372549019607843, "hmm_cov": 1.0, "evalue": 1.555473665578251e-07, "score": 25.797565460205078, "start": 16, "stop": 80 } ], "seq_stats": { "molecular_weight": 10995.937299999992, "isoelectric_point": 9.756339073181149 }, "id": "APKECPOBEN_250", "locus": "GCA002090215_00213" }, { "type": "cds", "sequence": "contig_1", "start": 124314, "stop": 124760, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGGTTTAATCCATGCAGGGATTTTGATGGTGTTAACTACCGTAGCACTTTCTAGTTTAGGTGGTTTGTTGTACTTACCTAAAGTTAATGCTTCCTATGACGGCAAGGGCTTTAAATCAATGGGGTGCTTACTGGAGTTTGCTGACTTAATTGCCACTTGGACTAGTGTCGGGATCTTTTGGTTCTTGGGCTTAGTTTTACTGGGTGGTTTATTGCAAATCAAGAAACCGAAGCGGTGGTATTTTAGAACAACGGGTTGGCTAGCTGTAGTAGTGATTGGTCTCACTACTTTAGTGGTCATGGTGCAACCGTTTGTAGATTTAGGAATAGCAGTGTTTAACCGTAGTTATGAACGGATTGTGGCTAACACTATTTTGATTGCTATTCTTGTGATAATTGTTTTAGTAATGTTCTTTCCAACCGAACCAATTAAGCTAAGACTTTGA", "aa": "MGLIHAGILMVLTTVALSSLGGLLYLPKVNASYDGKGFKSMGCLLEFADLIATWTSVGIFWFLGLVLLGGLLQIKKPKRWYFRTTGWLAVVVIGLTTLVVMVQPFVDLGIAVFNRSYERIVANTILIAILVIIVLVMFFPTEPIKLRL", "aa_hexdigest": "e330b724742793b6c8bf951155f9565f", "start_type": "GTG", "rbs_motif": "AATAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16314.708199999994, "isoelectric_point": 9.77651767730713 }, "id": "APKECPOBEN_251", "locus": "GCA002090215_00214" }, { "type": "cds", "sequence": "contig_1", "start": 124999, "stop": 125634, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAATTTAAGTATGGTGCGCTAATTTTTAGCGGCTTTTTAGGACTTTCAATAGTTCTAGCTAGTTGTGGGGCGAGGGGTAAATTTGATCAAGTTGATGATGGTAAGATTAAGCTAGCTTCATCTTTAACTTCTAGAAGTGCGTCAGCGGCTTTACAAAAAGTTGTTGAAAAGTACAACAAGGTTAAAGGTGTAAATGATTACCCCATTGAGATTACCCAGATTGCTGGTGGTTATGATGGCGGGCGAACTGATCTCCAAACCCGGGTTAGTGTAAAGGATAAAACCAGCTTTTACAACATGATTCTAAACTACCCTGATTTAGTGTCGGTGTTAGCCCGTAACGGGATGGAATTACCGTTTGATGGAGTCAATGTTGACAAGTTATCTCCCAACTTCTTAAAGTTCAATGAACGCATTAGTGGCGTAGCGAAAAAAGCTAACTATGCCATTCCCATTTCGATGTCAACCGATATCTTAATCCTGAATGCACCGGTGTTGCACTATATTTTGAACAGTGCTAAGAAGAATGATGGCAACACTAAAGTTCAAGTTAAAGCTCAGTCAAAAGATTCACAAACAAAAGTTAAAGGTACTATGGAAATTGGCACTGATGAAAGTACTAAAAACTTATGA", "aa": "MQFKYGALIFSGFLGLSIVLASCGARGKFDQVDDGKIKLASSLTSRSASAALQKVVEKYNKVKGVNDYPIEITQIAGGYDGGRTDLQTRVSVKDKTSFYNMILNYPDLVSVLARNGMELPFDGVNVDKLSPNFLKFNERISGVAKKANYAIPISMSTDILILNAPVLHYILNSAKKNDGNTKVQVKAQSKDSQTKVKGTMEIGTDESTKNL", "aa_hexdigest": "2d8a1f94c8c3146c140c06219fac1ab6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 22967.107100000016, "isoelectric_point": 9.421361351013182 }, "id": "APKECPOBEN_252", "locus": "GCA002090215_00215" }, { "type": "cds", "sequence": "contig_1", "start": 125875, "stop": 126624, "strand": "+", "frame": 1, "gene": null, "product": "P68 family surface lipoprotein", "db_xrefs": [ "GO:0005886", "GO:0009897", "SO:0001217", "UniRef:UniRef50_P75595", "UniRef:UniRef90_P75595" ], "nt": "TTGATCGATTTTTCAACGCGCGTAGCAAAATCATTTAAAAACAAAGTTAGTGATATTTCCACTAAAAAGGGCACTGACATTCAGGGCGTTTTAGGTCTAGACAGTACACCAAATGCTTTGTTTACCTCAGTTTTTGCTGCAGGTGATAGTAACTTTGATAACTTCTTTTATAAGGTTAAAGATGGTCGCGCTGACTTTAGTAACTTTAACGAAAACGGTACCTCTTACAAAAATCTGGAAAAGGTTTTTAATGACTATAAAAAACTCACAGACAGCAACGGTTTGTTTGTCAATAAAGGGGGTTCTTACACCTCTAACTTCCAGAAGTTTCACCAGTTAGCCTACAGCATTTCCTCCTCTTCTGGTTATGCTTATGCGTTTGCTGGTGAAAATTCAAAACGTTTAAAGTTCAACGACGATACTTTCATTGAGTACCCTAGTTTTACCCAGGAAATTCATGCGCCTGGTCAAAGTAGTCAAAAAGAAGGTGGACAGCAACAAAGCAACAGTAAAGATAACGGCAATCTTTTAGGCACTTTTACTATTGAAGCAGCGAAAAGTAAAACAAAAACAGAAGTTAAAAAAACTGAAGACACACAAAATCAAGGCAAGAAGGCTGAAGGCACACCTAACCAAGGTAAAAAAGCCGAAGGGACCGAAAACCAAGGTAAGACTATTTTCCTTTATAAAACATCAATTCCTAATGATAAGCAAGATGGTGTTGACGCTGTATTAATTAAAGACAAGTAG", "aa": "MIDFSTRVAKSFKNKVSDISTKKGTDIQGVLGLDSTPNALFTSVFAAGDSNFDNFFYKVKDGRADFSNFNENGTSYKNLEKVFNDYKKLTDSNGLFVNKGGSYTSNFQKFHQLAYSISSSSGYAYAFAGENSKRLKFNDDTFIEYPSFTQEIHAPGQSSQKEGGQQQSNSKDNGNLLGTFTIEAAKSKTKTEVKKTEDTQNQGKKAEGTPNQGKKAEGTENQGKTIFLYKTSIPNDKQDGVDAVLIKDK", "aa_hexdigest": "895fc2063a6d9c9b6b57d5f5d34dc806", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 125575, "stop": 126624, "observations": { "mutation": [ 125632, 125866 ], "stop": [ 125632, 125866 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75595", "query_cov": 1.0, "subject_cov": 0.4602587800369686, "identity": 0.996, "score": 425.0, "evalue": 3.96e-145, "gene_start": 1, "gene_end": 249, "reference_start": 293, "reference_end": 541, "reference_sequence": "MQFKYGALIFSGFLGLSIVLASCGARGKFDQVDDGKIKLASSLTSRSASAALQKVVEKYNKVKGVNDYPIEITQIAGGYDGGRTDLQTRVSVKDKTSFYNMILNYPDLVSVLARNGMELPFDGVNVDKLSPNFLKFNERISGVAKKANYAIPISMSTDILILNAPVLHYILNSAKKNDGNTKVQVKAQSKDSQTKVKGTMEIGTDESTKNLWSDIQKKAGENGKATTEGTKKAAAKSTHLTLLTKSEQSTQGNNGASESDKKIEETWGTYSEVDGGLKNYTFKADVFDTWHGLIDFSTRVAKSFKNKVSDISTKKGTDIQGVLGLDSTPNALFTSVFAAGDSNFDNFFYKVKDGRADFSNFNENGTSYKNLEKVFNDYKKLTDSNGLFVNKGGSYTSNFQKFHQLAYSISSSSGYAYAFAGENSKRLKFNDDTFIEYPSFTQEIHAPGQSSQKEGGQQQSNSKDNGNLLGTFTIEAAKSKTKTEVKKTEDTQNQGKKAEGTPNQGKKAEGTENQGKTIFLYKTSIPNDKQDGVDAVLIKDK" }, "paralog": false, "identity": 0.994269340974212, "score": 632.0, "evalue": 8.41e-229, "description": "Internal stop codon at 125632, 125866. Nonsense mutation around 125632, 125866.", "psc": { "uniref90_id": "UniRef90_P75595", "product": "P68 family surface lipoprotein", "uniref50_id": "UniRef50_P75595", "go_ids": [ "GO:0005886", "GO:0009897" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75595", "UniRef:UniRef50_P75595", "GO:0005886", "GO:0009897" ] }, "pscc": { "uniref50_id": "UniRef50_P75595", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75595" ], "product": "Uncharacterized lipoprotein MPN_097" } }, "id": "APKECPOBEN_253", "locus": "GCA002090215_00216" }, { "type": "cds", "sequence": "contig_1", "start": 126922, "stop": 127026, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATGACTCAAGGTCCTAATCTAATTGGTATCCATACTAATACCAAGGAAAATGAAGAAACGCAAAAGTTTGTTAACTGGTTCTTAAATACCAGTCTAACTTGA", "aa": "MMTQGPNLIGIHTNTKENEETQKFVNWFLNTSLT", "aa_hexdigest": "2047a8bc4f0b3da0607b140375041861", "start_type": "ATG", "rbs_motif": "AAAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3938.3996000000006, "isoelectric_point": 5.478164863586425 }, "id": "APKECPOBEN_254", "locus": "GCA002090215_00217" }, { "type": "cds", "sequence": "contig_1", "start": 127216, "stop": 127365, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGGTTACAGTGATCCAAGCGATTTCAGGGGTGGTAAATTCCGCGAATCAATTGGTTCTGCCTTTAACGCCACGGTCAATTCACACGTTGACTTTAATACTTTCGTTGCTAACTTCCGTGCTAACTTAGGTTCTGGTTACGATAAATAG", "aa": "MGYSDPSDFRGGKFRESIGSAFNATVNSHVDFNTFVANFRANLGSGYDK", "aa_hexdigest": "f1815f7d0d0feaaebcea9c9fb1c32008", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5352.7325, "isoelectric_point": 6.516329002380371 }, "id": "APKECPOBEN_255", "locus": "GCA002090215_00218" }, { "type": "cds", "sequence": "contig_1", "start": 127337, "stop": 127777, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W7Y3", "UniRef:UniRef90_A0AB38W7Y3" ], "nt": "GTGGTTTTCGTTGCCTTAGGTGTTATTCCCAGTAGCCTTATCATTCCTTCACCCCGCCCCGGTTCAGAGCTGGAGGTTGGCTTGGTCGAGGGTGGGTCATTTGGGAATATCTTTCCCCGTAGTCGTCGTCTGACTGCCCCCGAGGGTGGCTTGGACATTCGCTTTGAGACCGACGCCGACATTCGAAAACGTCGGGAGAAAGGAACGCGAACCACTTGTGTTCGTACTAGTTCCGCTGGACGACCCCGCGCCGGTTTTAGGCGCGGTTATATCATCCACACCCCGGTGATCATTCCCATACCTGTGCCCATTCCCAATCCAGGGAATATAATCCAACACCTGGTCGAGGAGTGCCCCCGCGAGGTTGAACCCGGTGGAGAAACGGGAAAGCGTGGGGGAGAAGTGGGATTAACTATTTATCGTAACCAGAACCTAAGTTAG", "aa": "MVFVALGVIPSSLIIPSPRPGSELEVGLVEGGSFGNIFPRSRRLTAPEGGLDIRFETDADIRKRREKGTRTTCVRTSSAGRPRAGFRRGYIIHTPVIIPIPVPIPNPGNIIQHLVEECPREVEPGGETGKRGGEVGLTIYRNQNLS", "aa_hexdigest": "5c79d429235ce5a31c71e84481a4d794", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0AB38W7Y3", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.993, "score": 280.0, "evalue": 3.15e-95, "valid": true, "uniref50_id": "UniRef50_A0AB38W7Y3" }, "pscc": { "uniref50_id": "UniRef50_A0AB38W7Y3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W7Y3" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15849.012899999983, "isoelectric_point": 9.865999794006349 }, "id": "APKECPOBEN_256", "locus": "GCA002090215_00219" }, { "type": "cds", "sequence": "contig_1", "start": 128069, "stop": 128350, "strand": "+", "frame": 2, "gene": null, "product": "Adhesin P1-like protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB33HTF6", "UniRef:UniRef90_A0AB33HTF6" ], "nt": "GTGTTGGTGAATCGAAGTGGGTCCGATTCCAACAAATTCCAAGCCACCGACCAAAAATGGTCCTACACCGACTTACAGTCGGACCAAACCAAACTGAACCTCTCCGCTTACGGTGAGGTGAATGGGTTGTTGAATCCGGCTTTGGTAGAAACCTATTTTGGGACGACGCGTACGTCTAGCACTGCGAACCAAAACAGTACAACCGTCCCCGGTATCGGTTTTAAAATTCCCGAACAAAATAATGATTCCAAAGCCACCCTGATCACCCCCGGGTTGGCTTGA", "aa": "MLVNRSGSDSNKFQATDQKWSYTDLQSDQTKLNLSAYGEVNGLLNPALVETYFGTTRTSSTANQNSTTVPGIGFKIPEQNNDSKATLITPGLA", "aa_hexdigest": "e42bb8ca944d16dfc92afce773255570", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 127997, "stop": 128646, "observations": { "deletion": [ 128426 ], "mutation": [ 128348 ], "stop": [ 128348, 128492 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB33HTF6", "query_cov": 1.010752688172043, "subject_cov": 0.42727272727272725, "identity": 0.862, "score": 155.0, "evalue": 7.97e-46, "gene_start": 1, "gene_end": 93, "reference_start": 25, "reference_end": 118, "reference_sequence": "MSFTNKNNAQRVQLFLRGLLGSIPVLVNKSGQDDNSKFNSTDQKWSYTELKSDQTKLNLPAYGEVNGLLNPALVETYFGTTLTSSTANQNSTTVPSIGFKIPEQNNDSKAVLITPGLAWTPQDVGNLVVSGTSLSFQLGGWLVTVHGLYQTPRGLFGPPVKWPGCQWQRPKGVNLGQAALSGISWQLGQPVGTGRECVGFEGGVGGSSSQRGVRVASSYK" }, "paralog": false, "identity": 0.8409090909090909, "score": 355.0, "evalue": 3.39e-127, "description": "Internal stop codon at 128348, 128492. Frameshift due to deletion around 128426. Nonsense mutation around 128348.", "psc": { "uniref90_id": "UniRef90_A0AB33HTF6", "product": "Adhesin P1 homolog", "uniref50_id": "UniRef50_A0AB33HTF6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB33HTF6", "UniRef:UniRef50_A0AB33HTF6" ] }, "pscc": { "uniref50_id": "UniRef50_A0AB33HTF6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB33HTF6" ], "product": "Adhesin P1 homolog" } }, "id": "APKECPOBEN_257", "locus": "GCA002090215_00220" }, { "type": "cds", "sequence": "contig_1", "start": 128393, "stop": 128533, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAGCTTCCAGCTCGGCGGGTGGCTGGTCACCTTCACGGACTTTGTCAAACCCCGCGCGGGTTACCTCGGGCTCCAGTTAAGTGGCCTGAATGCCAGTGACAGCGACCAAAGGGAGTTAATTTGGGCCCCCCGGCCCTGA", "aa": "MSFQLGGWLVTFTDFVKPRAGYLGLQLSGLNASDSDQRELIWAPRP", "aa_hexdigest": "f59ceb6ffaf11591e3d682ae927ab7e1", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5139.797100000002, "isoelectric_point": 5.9199743270874015 }, "id": "APKECPOBEN_258", "locus": "GCA002090215_00221" }, { "type": "cds", "sequence": "contig_1", "start": 128573, "stop": 129094, "strand": "+", "frame": 2, "gene": null, "product": "Putative adhesin P1-like protein MPN_099", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75593", "UniRef:UniRef90_P75593" ], "nt": "GTGGAGAGTGTGTGGGATTTGAAGGGGGTGTGGGCGGATCAAGCTCAGTTGGCCGCGCAAGCAGCTACAAGTAGTACCACCACCACCGCAACAGGGGCTACCTTACCGGAGCACCCGAATGCTTTGGCGTACCAAATTAGCTATACCGACAAGGATTCGTACAAGGCTTCCACTCAAGGTTCGGGTCAAACCAATTCCCAAAACAATTCGCTCTACCTCCATTTGATTAAACCTAAGAAAGTCGAAAGCACGACCCAACTCGACCAGGGCTTAAAAAACCTGTTGGACCCCAACCAGGTTCGCACCAAGCTGCGCCAAAGCTTTGGTACAGACCATTCCACCCAGCCCCAGCCCCAATCGCTCAAAACAACGACACCGGTGTTTGGAGCCATGAGTGGTAACCTCGGCAGTGTGCTTAGTGGTGGGGGTGCTGGAGGAGCAGGCAGCACCAATTCGGTGGACCTCTCCCCCGTTGAACGGGTGAGTGGGTCACTAACCATTAATAGGAATTTTTCTTATTAA", "aa": "MESVWDLKGVWADQAQLAAQAATSSTTTTATGATLPEHPNALAYQISYTDKDSYKASTQGSGQTNSQNNSLYLHLIKPKKVESTTQLDQGLKNLLDPNQVRTKLRQSFGTDHSTQPQPQSLKTTTPVFGAMSGNLGSVLSGGGAGGAGSTNSVDLSPVERVSGSLTINRNFSY", "aa_hexdigest": "f8adead0ef04905a018b966c5bc09401", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 128273, "stop": 129094, "observations": { "mutation": [ 128348, 128531 ], "stop": [ 128348, 128531 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75593", "query_cov": 1.0, "subject_cov": 0.4357682619647355, "identity": 0.96, "score": 316.0, "evalue": 2.07e-105, "gene_start": 1, "gene_end": 173, "reference_start": 225, "reference_end": 397, "reference_sequence": "TTQDGNATGQQEATNYTNLPPNLTPTADWPNALSFTNKNNAQRAQLFLRGLLGSIPVLVNRSGSDSNKFQATDQKWSYTDLQSDQTKLNLSAYGEVNGLLNPALVETYFGTTRTSSTANQNSTTVPGIGFKIPEQNNDSKATLITPGLAWTPQDVGNLVVSGTTVSFQLGGWLVTFTDFVKPRAGYLGLQLSGLDASDSDQRELIWAPRPWAAFRGSWVNRLGRVESVWDLKGVWADQAQLAAQAATSSATTTATGATLPEHPNALAYQISYTDKDSYKASTQGSGQTNSQNNSLYLHLIKPKKVESTTQLDQGLKNLLDPNQVRTKLRQSFGTDHSTQPQPQSLKTTTPVFGAMSGNLGSVLSGGGAGGAGSTNSVDLSPVERVSGSLTINRNFSY" }, "paralog": false, "identity": 0.9597069597069597, "score": 520.0, "evalue": 4.51e-188, "description": "Internal stop codon at 128348, 128531. Nonsense mutation around 128348, 128531.", "psc": { "uniref90_id": "UniRef90_P75593", "product": "Putative adhesin P1-like protein MPN_099", "uniref50_id": "UniRef50_P75593", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75593", "UniRef:UniRef50_P75593" ] }, "pscc": { "uniref50_id": "UniRef50_P75593", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75593" ], "product": "Putative adhesin P1-like protein MPN_099" } }, "id": "APKECPOBEN_259", "locus": "GCA002090215_00222" }, { "type": "cds", "sequence": "contig_1", "start": 129182, "stop": 129433, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_015344874.1", "SO:0001217", "UniParc:UPI0002B8420C", "UniRef:UniRef100_A0AB38WA43", "UniRef:UniRef50_A0A0H3DNZ5", "UniRef:UniRef90_A0A0H3DNZ5" ], "nt": "ATGAAATTAGTTAAATTAACGAGGCTTCACCGCTTTTTAAAACAAGTTCAATTGCAACTTGTTCCTTTGTTTAGCCAAAACTTTTTGCAAGTTTTATTGGCGCTTCTCAATAAACCGGTCAATTTATTTAGTGAAAACACAACAATGAAAACAAATAAAAAATACGCCTTTGCAGCGTTGCTAATGCAAGCTCAAGCTAAGCTCCCAAATTCATTTAAATTCTTTCTTTCAAAGAAAGGAGGTATGGCTTAA", "aa": "MKLVKLTRLHRFLKQVQLQLVPLFSQNFLQVLLALLNKPVNLFSENTTMKTNKKYAFAALLMQAQAKLPNSFKFFLSKKGGMA", "aa_hexdigest": "18d85a11dacc9036768befe2855820f5", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0002B8420C", "ncbi_nrp_id": "WP_015344874.1", "uniref100_id": "UniRef100_A0AB38WA43", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002B8420C", "RefSeq:WP_015344874.1", "UniRef:UniRef100_A0AB38WA43" ] }, "ips": { "uniref100_id": "UniRef100_A0AB38WA43", "uniref90_id": "UniRef90_A0A0H3DNZ5" }, "psc": { "uniref90_id": "UniRef90_A0A0H3DNZ5", "uniref50_id": "UniRef50_A0A0H3DNZ5" }, "pscc": { "uniref50_id": "UniRef50_A0A0H3DNZ5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0H3DNZ5" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9528.455399999993, "isoelectric_point": 10.966668510437014 }, "id": "APKECPOBEN_260", "locus": "GCA002090215_00223" }, { "type": "cds", "sequence": "contig_1", "start": 129601, "stop": 129984, "strand": "+", "frame": 1, "gene": null, "product": "UPF0134 protein MPN_100", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75254", "UniRef:UniRef90_P75592" ], "nt": "GTGAACACGGAATACGTAACGCGTAAAGAATTTAACGAATATAAAGTTTCAAACGACCAACGGCTTACAAAGATTGAAACCACTTTGGCCGCCCAAGGTGAACAAATCAACAAATTGACTCAAACTGTTGAAAAACAAGGCGAGCAAATCAATCAATTAGTTCAAGTTGTGCTTCTTCACGGTGAACAAATCAACAAATTGACTCAAACTGTTGAAAAACAGGGCGAGCAAATTAAAGAACTTCAAGTGGAGCAAAAAGCTCAGGGTGAGCAAATTAAAGCTCAAGGAAAACAAATAAAAGCTCAAGGAAAAACGCTTAAGTCGATCCTACAAGCACTTGGAGGAATAAATAAACGCTTGGACAAAATTGATCCACCTAAATAG", "aa": "MNTEYVTRKEFNEYKVSNDQRLTKIETTLAAQGEQINKLTQTVEKQGEQINQLVQVVLLHGEQINKLTQTVEKQGEQIKELQVEQKAQGEQIKAQGKQIKAQGKTLKSILQALGGINKRLDKIDPPK", "aa_hexdigest": "85d68744f8cb31193f82e85073865a5b", "start_type": "GTG", "rbs_motif": "AACAA", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 129433, "stop": 129984, "observations": { "mutation": [ 129514 ], "stop": [ 129514 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75592", "query_cov": 1.0, "subject_cov": 0.6939890710382514, "identity": 0.992, "score": 95.9, "evalue": 1.9e-22, "gene_start": 1, "gene_end": 127, "reference_start": 57, "reference_end": 183, "reference_sequence": "MKEKIPFYNEKEFHDMVKKTKKGTFSGWYIIDKDNKSVEFSGSFNRQFKLNKPVIPVNTEYVTRKEFNEYKVSNDQRLTKIETTLAAQGEQINKLTQTVEKQGEQINQLVQVVLLHGEQINKLTQTVEKQGEQIKELQVEQKAQGEQIKAQGKQIKAQGKTLKSILQALGGINKRLDKIDPPK" }, "paralog": false, "identity": 0.994535519125683, "score": 211.0, "evalue": 2.37e-70, "description": "Internal stop codon at 129514. Nonsense mutation around 129514.", "psc": { "uniref90_id": "UniRef90_P75592", "product": "UPF0134 protein MPN_100", "uniref50_id": "UniRef50_P75254", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75592", "UniRef:UniRef50_P75254" ] }, "pscc": { "uniref50_id": "UniRef50_P75254", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75254" ], "product": "UPF0134 protein MPN_524" } }, "id": "APKECPOBEN_261", "locus": "GCA002090215_00224" }, { "type": "cds", "sequence": "contig_1", "start": 130441, "stop": 130698, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF12378.14" ], "nt": "GTGCGCGACAACAGCGCCAAGGGCATCACTGCCGGTAGTGAAAGTCAACAAACCACGTATGATCCCACTCGAACCGAAGCGGCTTTGACCGCATCAACCACCTTTGCGTTACGCCGGTATGACCTCGCCGGGCGCGCCTTATACGACCTCGACTTTAGTAGGTTAAACCCACAGACACCAACGCGCGACCAAACCGGGCAGATCACCTTTAACCCCTTTGGCGGCTTTGGTTTGAGTGGGGCTGCACCCCAACAGTGA", "aa": "MRDNSAKGITAGSESQQTTYDPTRTEAALTASTTFALRRYDLAGRALYDLDFSRLNPQTPTRDQTGQITFNPFGGFGLSGAAPQQ", "aa_hexdigest": "b905c138d040e7b9e91ea82d31269fa1", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF12378.14", "name": "P1_N", "length": 58, "aa_cov": 0.6823529411764706, "hmm_cov": 0.896551724137931, "evalue": 4.894433937602778e-09, "score": 31.38545799255371, "start": 27, "stop": 84 } ], "seq_stats": { "molecular_weight": 9190.931099999994, "isoelectric_point": 6.072189140319824 }, "id": "APKECPOBEN_262", "locus": "GCA002090215_00225" }, { "type": "cds", "sequence": "contig_1", "start": 130729, "stop": 131247, "strand": "+", "frame": 1, "gene": null, "product": "putative protein MPN_101", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75571", "UniRef:UniRef90_P75568" ], "nt": "GTGGCCCAAGACCCCTCCAATCCTTATCGGTTTGCCGTTTTACTCGTGCCGCGTAGCGTGGTGTACTATGAGCAGTTGCAGCGGGGGTTAGCGCTCCCTAACCAAGGGAGTTCGTCAGGCTCCGGTCAACAAAACACCACCATTGGCGCGTATGGGCTGAAGGTGAAGAACGCCGAGGCGGACACCGCGAAGAGCAATGAAAAACTCCAGGGCGATGAATCCAAGTCTTCCAATGGATCTTCAAGCACTTCCACCACCACCCAACGTGGGGGTTCGTCAGGGGACACCAAAGTCAAGGCGTTGCAGGTGGCGGTGAAAAAGAAATCCGGGAGTCAGGGCAACTCCGGTGAACAAGGCACCGAACAGGTGGAACTTGAATCTAATGATCTCGCCAACGCTCCCATTAAGCGGGGCGAGGAGTCGGGTCAGTCCGTCCAACTAAAGGCAGCCGACTTCGGCACCACCCCATCCAGTTCGGGATCAGGCGGCAACTCCAACCCCGGTTCCCCCACCCCCTGA", "aa": "MAQDPSNPYRFAVLLVPRSVVYYEQLQRGLALPNQGSSSGSGQQNTTIGAYGLKVKNAEADTAKSNEKLQGDESKSSNGSSSTSTTTQRGGSSGDTKVKALQVAVKKKSGSQGNSGEQGTEQVELESNDLANAPIKRGEESGQSVQLKAADFGTTPSSSGSGGNSNPGSPTP", "aa_hexdigest": "deafec41f99b852a76dea4743f7ffc63", "start_type": "GTG", "rbs_motif": "AACAA", "genes": [], "truncated": "both", "pseudogene": { "start": 130441, "stop": 131550, "observations": { "mutation": [ 130696, 131245, 131293, 131407, 131422, 131446 ], "stop": [ 130696, 131245, 131293, 131407, 131422, 131446 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75568", "query_cov": 1.0, "subject_cov": 0.40186915887850466, "identity": 0.977, "score": 253.0, "evalue": 1.55e-80, "gene_start": 1, "gene_end": 172, "reference_start": 97, "reference_end": 268, "reference_sequence": "MRDNSAKGITAGSESQQTTYDPTRTEAALTASTTFALRRYDLAGRALYDLDFSRLNPQTPTRDQTGQITFNPFGGFGLSGAAPQQWNEVKNKVPVEVAQDPSNPYRFAVLLVPRSVVYYEQLQRGLALPNQGSSSGSGQQNTTIGAYGLKVKNAEADTAKSNEKLQGDESKSSNGSSSTSTTTQRGGSSGDTKVKALQVAVKKKSGSQGNSGEQGTEQVELESNDLANAPIKRGEESGQSVQLKAADFGTTPSSSGSGGNSNPGSPTPWRPWLATEQIHKDLPKWSASILILYDAPYARNRTAIDRVDHLDPKVMTANYPPSWRTPKWNHHGLWDWKARDVLLQTTGFFNSRRHPEWFDQGQAVADNTQTGFDTDDTDNKKTRLSKGSWLRQAGPDRPPVWSVLRQHWQPHLVRASAFGVWDLFVLIN" }, "paralog": false, "identity": 0.9728997289972899, "score": 655.0, "evalue": 3.62e-241, "description": "Internal stop codon at 130696, 131245, 131293, 131407, 131422, 131446. Nonsense mutation around 130696, 131245, 131293, 131407, 131422, 131446.", "psc": { "uniref90_id": "UniRef90_P75568", "product": "Uncharacterized protein MPN_101", "uniref50_id": "UniRef50_P75571", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75568", "UniRef:UniRef50_P75571" ] }, "pscc": { "uniref50_id": "UniRef50_P75571", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75571" ], "product": "Uncharacterized protein MPN_205" } }, "id": "APKECPOBEN_263", "locus": "GCA002090215_00226" }, { "type": "cds", "sequence": "contig_1", "start": 131209, "stop": 131547, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGGTATTATCCGCGACCGCTTGGCCCTGGTCAAACCACTCGGGGTGGCGCCGCGAGTTGAAGAACCCGGTGGTTTGGAGCAAAACATCTCTGGCCTTTCAGTCCCACAACCCGTGGTGGTTTCACTTGGGCGTTCTTCAACTGGGCGGATAGTTCGCGGTCATCACCTTGGGATCCAAGTGATCAACGCGATCAATGGCGGTACGATTGCGCGCATAAGGCGCATCGTACAGAATCAGGATCGAGGCGGATCATTTGGGGAGGTCCTTGTGAATTTGCTCGGTCGCAAGCCACGGCCTTCAGGGGGTGGGGGAACCGGGGTTGGAGTTGCCGCCTGA", "aa": "MGIIRDRLALVKPLGVAPRVEEPGGLEQNISGLSVPQPVVVSLGRSSTGRIVRGHHLGIQVINAINGGTIARIRRIVQNQDRGGSFGEVLVNLLGRKPRPSGGGGTGVGVAA", "aa_hexdigest": "7cf9296612bd24329f3b776b89af0cfd", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11527.21639999999, "isoelectric_point": 11.8579439163208 }, "id": "APKECPOBEN_264", "locus": "GCA002090215_00227" }, { "type": "cds", "sequence": "contig_1", "start": 131564, "stop": 131719, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_308009835.1", "SO:0001217", "UniParc:UPI0027E13561", "UniRef:UniRef100_UPI002FE6C792", "UniRef:UniRef50_UPI002FE6C792", "UniRef:UniRef90_UPI002FE6C792", "PFAM:PF12378.14" ], "nt": "ATGACACCGATAATAAAAAAACAAGGCTTTCAAAAGGAAGCTGACTCCGACAAGCCGGCCCCGATCGCCCTCCCGTTTGAAGCGTACTTCGCCAACATTGGCAACCTCACCTGGTTCGGGCAAGCGCTTTTGGTGTTTGGGATTTGTTTGTCTTAA", "aa": "MTPIIKKQGFQKEADSDKPAPIALPFEAYFANIGNLTWFGQALLVFGICLS", "aa_hexdigest": "44efda8d28e7a050d96dbc16ff2a8696", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0027E13561", "ncbi_nrp_id": "WP_308009835.1", "uniref100_id": "UniRef100_UPI002FE6C792", "db_xrefs": [ "SO:0001217", "UniParc:UPI0027E13561", "RefSeq:WP_308009835.1", "UniRef:UniRef100_UPI002FE6C792" ] }, "ips": { "uniref100_id": "UniRef100_UPI002FE6C792", "uniref90_id": "UniRef90_UPI002FE6C792" }, "psc": { "uniref90_id": "UniRef90_UPI002FE6C792", "uniref50_id": "UniRef50_UPI002FE6C792" }, "pscc": { "uniref50_id": "UniRef50_UPI002FE6C792", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FE6C792" ] }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF12378.14", "name": "P1_N", "length": 36, "aa_cov": 0.6862745098039216, "hmm_cov": 1.0, "evalue": 5.0777345674360556e-09, "score": 31.333059310913086, "start": 13, "stop": 47 } ], "seq_stats": { "molecular_weight": 5601.493899999998, "isoelectric_point": 5.968457984924316 }, "id": "APKECPOBEN_265", "locus": "GCA002090215_00228" }, { "type": "cds", "sequence": "contig_1", "start": 131874, "stop": 132311, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGGACAGAACAAGTTGGTGGCGCTGGTCCGCTCAGCGTTGGTCAGGGTGAGGCTTAATGATGGGACGAGTTCGGATTCCAATACCACCACTGATGGGGCGCTTACGTTTGACACGAATGAAAGTCAAAAAGCTTTAGGCTCCACGGACACGCAGGGTTCCGGGTCTAACTCCCAAGACTTCGCCAGCTACATCCTCATCTTTAAAGCCGCGCCCAGGGCCACGTGGGTGTTTGAACGGAAGATTAAGTTGGCGTTGCCCTACGTTAAAAACGAATCGGGAGCAGGGGACTCCACCACAACTAACAGTGGCTCCCTCTACAAAACCCTCCAAGACCTCCTCGTCGAACAACCCGTGACCCCTTACACGCCCAATGCGGGGTTAGCCCGGGTGAATGGGGTTGCTCAGGACAAGGTGCATGGGTTGGAGTCTGGGTGA", "aa": "MGQNKLVALVRSALVRVRLNDGTSSDSNTTTDGALTFDTNESQKALGSTDTQGSGSNSQDFASYILIFKAAPRATWVFERKIKLALPYVKNESGAGDSTTTNSGSLYKTLQDLLVEQPVTPYTPNAGLARVNGVAQDKVHGLESG", "aa_hexdigest": "fca8adc49ca3ff2021bc087765450749", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15382.876899999977, "isoelectric_point": 6.564755821228027 }, "id": "APKECPOBEN_266", "locus": "GCA002090215_00229" }, { "type": "cds", "sequence": "contig_1", "start": 132522, "stop": 133037, "strand": "+", "frame": 3, "gene": "mgpC", "product": "MgpC family cytadherence protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0028006D2D" ], "nt": "GTGGGATCAACCGGGAGTCAAATGGCTTCTAGTACCAGTTTGGGCAATGTTGGGGACACCACTGCCCTAACTCCACTTTTAAGAGGTGCTACTACTACTACTACTACCGTCCAGCTAAAACAAACAGATTCTAACAGTCAAGATCAACAAAAATTCCAAAAATATTTAAACACCGCCCAGGCCCTGCACCAGATGGGGGTGATAGTGCCAGAACTTAGCCAAGGCGGTTGGCAAAGCCAAACAGCGCGGCACAGTTCCACCCGGGGACTACAGTCAGCCGGGGCCAATCGTGGCGCTAGTTTAGCGGCACGCACCACCACAACTGTGGGGGTGGGTAGAAGAGATTCCACTTCCACCACCTTGGAACTACCAAACGTCATCACCCAGCTTTATCATACCTCCACGTCCCAACTCGCTTACTTAAATGGCCAGATCGTTGTGATGGGTTCAAACGCGGTACCGAGTTTGTGGTATTGGGTCGTGGATGAGCGAACCACGTCCGGGAGGGCGACTTGA", "aa": "MGSTGSQMASSTSLGNVGDTTALTPLLRGATTTTTTVQLKQTDSNSQDQQKFQKYLNTAQALHQMGVIVPELSQGGWQSQTARHSSTRGLQSAGANRGASLAARTTTTVGVGRRDSTSTTLELPNVITQLYHTSTSQLAYLNGQIVVMGSNAVPSLWYWVVDERTTSGRAT", "aa_hexdigest": "1b91fcf4cd9cd9197a21a87dffa5ede8", "start_type": "GTG", "rbs_motif": "AAAA", "psc": { "uniref90_id": "UniRef90_UPI0028006D2D", "query_cov": 0.9883040935672515, "subject_cov": 0.9825581395348837, "identity": 0.562, "score": 141.0, "evalue": 1.1e-39, "valid": false, "product": "MgpC family cytadherence protein", "uniref50_id": "UniRef50_UPI0028006D2D" }, "pscc": { "uniref50_id": "UniRef50_UPI0028006D2D", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0028006D2D" ], "product": "MgpC family cytadherence protein" }, "genes": [ "mgpC" ], "id": "APKECPOBEN_267", "locus": "GCA002090215_00230" }, { "type": "cds", "sequence": "contig_1", "start": 133053, "stop": 133346, "strand": "+", "frame": 3, "gene": "mgpC", "product": "MgpC family cytadherence protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F374488" ], "nt": "TTGAACTTCGGCACTGAAGTTCAAAAAAACTTCGTCGAAAACCAGTTGGGGTTTAAATCCGAAGACAACTCCAACACCTCCTTGACGAACTTCAAGAGTCAAGGCTTAACTCAGCCAGCTTACTTAATCAGTGGCCTTGACGTTGTGGCCGACCACCTCGTCTTTGCGGCCTTTAAAGCGGGCGCGGTGGGGTATGATATGACGACTGATTCGAACGCTTCGACCTACAACCAAGCGCTCACCTGGTCGACCACGGCCGGGTTGGACAGTGATGGGGGGACAACAACCTGGTGA", "aa": "MNFGTEVQKNFVENQLGFKSEDNSNTSLTNFKSQGLTQPAYLISGLDVVADHLVFAAFKAGAVGYDMTTDSNASTYNQALTWSTTAGLDSDGGTTTW", "aa_hexdigest": "49056614286de90ee6a3ce94660f6d75", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001F3174D6", "query_cov": 0.8556701030927835, "subject_cov": 0.8217821782178217, "identity": 0.795, "score": 132.0, "evalue": 3.51e-38, "valid": false, "product": "MgpC family cytadherence protein", "uniref50_id": "UniRef50_UPI001F374488" }, "pscc": { "uniref50_id": "UniRef50_UPI001F374488", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F374488" ], "product": "MgpC family cytadherence protein" }, "genes": [ "mgpC" ], "id": "APKECPOBEN_268", "locus": "GCA002090215_00231" }, { "type": "cds", "sequence": "contig_1", "start": 133343, "stop": 133585, "strand": "+", "frame": 2, "gene": "mgpC", "product": "MgpC protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W8G0", "UniRef:UniRef90_A0AB38W8G0" ], "nt": "GTGACGAATACTGCGGGGTTAAACGGTCCGATTAACGGCTTGTTTACCCTGTTAGACACCTTTGCCTATGTGACCCCGGTGAGTGGGATGAAAGGGGGGAGTAAGAATAACGAAGAAGTGCAAACGACCTACCCGGTCAAGTCCGACCAAAAGGCCACCGCCAAAATTGCGTCCTTAATTAATGCTTCGCCCTTAAACAGTTATGGGGATGATGGCGATAAAAAAGCCATCCACCTAAGGTGA", "aa": "MTNTAGLNGPINGLFTLLDTFAYVTPVSGMKGGSKNNEEVQTTYPVKSDQKATAKIASLINASPLNSYGDDGDKKAIHLR", "aa_hexdigest": "2bb4184595bdbdf888e44576516c3fcd", "start_type": "GTG", "rbs_motif": null, "genes": [ "mgpC" ], "truncated": "both", "pseudogene": { "start": 133295, "stop": 133641, "observations": { "deletion": [ 133577 ], "mutation": [ 133322 ], "stop": [ 133322 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB38W8G0", "query_cov": 0.95, "subject_cov": 0.6608695652173913, "identity": 0.9740000000000001, "score": 151.0, "evalue": 6.4e-46, "gene_start": 3, "gene_end": 78, "reference_start": 19, "reference_end": 94, "reference_sequence": "MVDHGRVGQWWGDKALVENTAGLNGPINGLFTLLDTFAYVTPVSGMKGGSQNNEEVQTTYPVKSDQKATAKIASLINASPLNSYGDDGDKKAIYPEVRGRQLQNIFDDWKTNKSC" }, "paralog": false, "identity": 0.9130434782608695, "score": 212.0, "evalue": 1.92e-72, "description": "Internal stop codon at 133322. Frameshift due to deletion around 133577. Nonsense mutation around 133322.", "psc": { "uniref90_id": "UniRef90_A0AB38W8G0", "product": "MgpC protein", "uniref50_id": "UniRef50_A0AB38W8G0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB38W8G0", "UniRef:UniRef50_A0AB38W8G0" ] }, "pscc": { "uniref50_id": "UniRef50_A0AB38W8G0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W8G0" ], "product": "MgpC protein" } }, "id": "APKECPOBEN_269", "locus": "GCA002090215_00232" }, { "type": "cds", "sequence": "contig_1", "start": 133662, "stop": 133880, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W8B4", "UniRef:UniRef90_A0AB38W8B4" ], "nt": "ATGAATTTACTAAATTTATTTAAATCAGCGGGAGCAGGCTCGTTTTTTCAACACAAGTCACTCTACCTGCTCCCACTAAATAAATTGAGACAGATTTTCAAAGTAGCCAAAGAGTTTGAATATTTTTGCAATTTGGGTTTACAACTCCATCTAACTATTAGTAGGGTTTTCACTTTTGAAATTTTTTTTGGATTAATTTTGGCACCCCGCCTTTTTTAA", "aa": "MNLLNLFKSAGAGSFFQHKSLYLLPLNKLRQIFKVAKEFEYFCNLGLQLHLTISRVFTFEIFFGLILAPRLF", "aa_hexdigest": "73a973a8d70155e29cc2e59ee515656c", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0AB38W8B4", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.986, "score": 139.0, "evalue": 4.76e-42, "valid": true, "uniref50_id": "UniRef50_A0AB38W8B4" }, "pscc": { "uniref50_id": "UniRef50_A0AB38W8B4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W8B4" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8414.970199999992, "isoelectric_point": 9.897847175598145 }, "id": "APKECPOBEN_270", "locus": "GCA002090215_00233" }, { "type": "cds", "sequence": "contig_1", "start": 134489, "stop": 134875, "strand": "+", "frame": 2, "gene": null, "product": "DUF16 domain-containing protein", "db_xrefs": [ "RefSeq:WP_080713773.1", "SO:0001217", "UniParc:UPI0009B8DF05", "UniRef:UniRef100_UPI0009B8DF05", "UniRef:UniRef50_UPI000476AE6E", "UniRef:UniRef90_UPI0009B8DF05" ], "nt": "GTGCCTAAACCAGATAAGTATGTAACACATCGCGAGCTTGATGAGAAACTTACTGAGTTTCATGACAAGCTTATGGTAGAACTTGATGGTAAGTTTGCTACTAAAGCTGATTTAAAACGGGTTGAAGATAAAGTTGATGTTTTGTTTGAATTGCAAAAAACGCAAGGTGAACAAATCAAGGTTCAAGGAAAACAAATCGAACAGTTAACTGAAACTGTTCAAAAACAGGGCGAACAAATAAAGGAACTTCAAGTTCAGGTAAAAGCCCAAGGTGAAGAAATAAAAGAAATTAAAGTTGAACAAAAAGCTCAAGGCCAAACCTTGCAGTTAATTCTGAAAGCACTTGAAGGAATAAACAAGCGACTAGATAATTTGGAATCGAAATAA", "aa": "MPKPDKYVTHRELDEKLTEFHDKLMVELDGKFATKADLKRVEDKVDVLFELQKTQGEQIKVQGKQIEQLTETVQKQGEQIKELQVQVKAQGEEIKEIKVEQKAQGQTLQLILKALEGINKRLDNLESK", "aa_hexdigest": "c0cfff194d68e57f064d0928ebb64f94", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0009B8DF05", "ncbi_nrp_id": "WP_080713773.1", "uniref100_id": "UniRef100_UPI0009B8DF05", "db_xrefs": [ "SO:0001217", "UniParc:UPI0009B8DF05", "RefSeq:WP_080713773.1", "UniRef:UniRef100_UPI0009B8DF05" ] }, "ips": { "uniref100_id": "UniRef100_UPI0009B8DF05", "uniref90_id": "UniRef90_UPI0009B8DF05" }, "psc": { "uniref90_id": "UniRef90_UPI0009B8DF05", "product": "DUF16 domain-containing protein", "uniref50_id": "UniRef50_UPI000476AE6E" }, "pscc": { "uniref50_id": "UniRef50_UPI000476AE6E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000476AE6E" ], "product": "DUF16 domain-containing protein" }, "genes": [], "id": "APKECPOBEN_271", "locus": "GCA002090215_00234" }, { "type": "cds", "sequence": "contig_1", "start": 135072, "stop": 135251, "strand": "+", "frame": 3, "gene": null, "product": "Family K-like protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAN5BCY4", "UniRef:UniRef90_A0AAN5BCY4" ], "nt": "ATGAAAGATGAAACAAATATTTGTTCTCAATCTTTAAAAAGACCTTTAAGCGAAGACCAATTGATAGAGTATTTGAGTACTTATGAAATAACTTTATTTGAGGTTTATGCTGAGGATATGCTAGATGGCAATTGCAAAATTTATATACATGCTAAAGGACGTCCAAAAGACCCAGATTGA", "aa": "MKDETNICSQSLKRPLSEDQLIEYLSTYEITLFEVYAEDMLDGNCKIYIHAKGRPKDPD", "aa_hexdigest": "402782f5382e4b9b10aba387c56fea46", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 135072, "stop": 135338, "observations": { "mutation": [ 135249 ], "stop": [ 135249 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAN5BCY4", "query_cov": 1.0, "subject_cov": 0.6704545454545454, "identity": 1.0, "score": 122.0, "evalue": 2.25e-35, "gene_start": 1, "gene_end": 59, "reference_start": 1, "reference_end": 59, "reference_sequence": "MKDETNICSQSLKRPLSEDQLIEYLSTYEITLFEVYAEDMLDGNCKIYIHAKGRPKDPDWKRKTDKKSLEIERYVKKFIKFLKREQKK" }, "paralog": false, "identity": 0.9886363636363636, "score": 177.0, "evalue": 3.52e-59, "description": "Internal stop codon at 135249. Nonsense mutation around 135249.", "psc": { "uniref90_id": "UniRef90_A0AAN5BCY4", "product": "Family K-like protein", "uniref50_id": "UniRef50_A0AAN5BCY4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAN5BCY4", "UniRef:UniRef50_A0AAN5BCY4" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAN5BCY4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAN5BCY4" ], "product": "Family K-like protein" } }, "id": "APKECPOBEN_272", "locus": "GCA002090215_00235" }, { "type": "cds", "sequence": "contig_1", "start": 135892, "stop": 136734, "strand": "+", "frame": 1, "gene": "pheS", "product": "phenylalanine--tRNA ligase subunit alpha", "db_xrefs": [ "COG:COG0016", "COG:J", "EC:6.1.1.20", "GO:0000049", "GO:0000166", "GO:0000287", "GO:0004826", "GO:0005524", "GO:0005737", "GO:0006432", "KEGG:K01889", "SO:0001217", "UniRef:UniRef50_P75564", "UniRef:UniRef90_P75564" ], "nt": "TTGGAAAGCACCTTGCAAACACTCCATGATACACAGCTACAAGTCTTTACCCAAGCCCAGTCTAGCAGTGTATTAACTCATGGTGATGTGATGTTGCTAGCAACCTCTTTTGCCCCTGGTTCTAGCAACATTATTTACCAGGTAATTGATGAGTTAGTGAACTATTTTAAGAAGTTCTTGTTTACGGTTAACTATGACAGTGAATTGACTACTATTGCAGACTGTTTTGACCTGCTAAACATTCCCAAAGACCATCCTAGTCGTAACCTTACTGACACCTTTTACTTAGACAAAAACCGCTTACTGAGAACACATTGTACTGCTGCTACTTTACGTGCTGTTAAGGAAACGAAAAAGAGTAATAACCCCGATATTCGGATTGCTTCCTTTGGTGCTGTTTTTCGTAAAGATGATGACGATGCCACCCACTCACACCAGTTCAACCAACTGGACTTCATGTGGATTAAAAAGGATTTTTCTTTAACTAACCTCAAGTGGTTTATGCAGAATATGATTAACCACATCTTTGGCGAAAATACTTCAGCGCGTTTTCGTCTGTCCCACTTCCCTTTTACCGAACCTTCCTTTGAAATTGACATCCGTTGCTGGTTGTGTCAGAATGGTTGTGGTGTATGTAAGAAAACACGTTGGATTGAGGTATTAGGGGCGGGTATCCTCCACCCTCAAGTGATGGCTAACATGGGCTTTAGTGACACGGATAATATAAGGGGTATAGCAGCTGGCATTGGGATTGAGCGGTTGGTAATGTTGAAACACGGCATTAGTGACATCCGTGACCTTTACGACAATAACTTCAAATTTTTAGCCCAGTTTACTGATTAG", "aa": "MESTLQTLHDTQLQVFTQAQSSSVLTHGDVMLLATSFAPGSSNIIYQVIDELVNYFKKFLFTVNYDSELTTIADCFDLLNIPKDHPSRNLTDTFYLDKNRLLRTHCTAATLRAVKETKKSNNPDIRIASFGAVFRKDDDDATHSHQFNQLDFMWIKKDFSLTNLKWFMQNMINHIFGENTSARFRLSHFPFTEPSFEIDIRCWLCQNGCGVCKKTRWIEVLGAGILHPQVMANMGFSDTDNIRGIAAGIGIERLVMLKHGISDIRDLYDNNFKFLAQFTD", "aa_hexdigest": "8632576b4a53936657e85c6b3d35df72", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75564", "query_cov": 1.0, "subject_cov": 0.8211143695014663, "identity": 0.996, "score": 567.0, "evalue": 1.53e-203, "valid": true, "gene": "pheS", "product": "phenylalanine--tRNA ligase subunit alpha", "ec_ids": [ "6.1.1.20" ], "uniref50_id": "UniRef50_P75564", "cog_id": "COG0016", "cog_category": "J", "kegg_orthology_id": "K01889", "go_ids": [ "GO:0000049", "GO:0000166", "GO:0000287", "GO:0004826", "GO:0005524", "GO:0005737", "GO:0006432" ] }, "pscc": { "uniref50_id": "UniRef50_P75564", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75564" ], "product": "Phenylalanine--tRNA ligase alpha subunit" }, "genes": [ "pheS" ], "id": "APKECPOBEN_273", "locus": "GCA002090215_00236" }, { "type": "cds", "sequence": "contig_1", "start": 136737, "stop": 137468, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01588.26" ], "nt": "ATGTTAATTTCTAAAAAAACTTTAGCTGTTTTAATTCCCGAAATTACCCACGTTTCCAACAATGAAATTTGTGAAAAGTTACAGCAAATCGGGATTGAAGTGGAAGCAATTCGCGCCTTTAAAAACCCTGATTACTTACAGTTGGGTATTCTCCGCGCTGTTACCCCCCACCCCCATGACAACCACTTGTATGTGTGTCAAGTTCAAATAGACAAAAACAAACAGCTCAATGTGGTGACTGGGGCAGTTAACATAGTTGATCCCAATAATCTCAATAAACACGTGATTGTCGCCAAAAAAGGGGCAGAACTGCTCAATGGTTTAATCATTAAAACGAAGAACATTAAGGGAATTATCTCGGACGGGATGTTGTGTAGTTATGTTGATATTAATCCGTTTAGTAAGCATTTAATTGCTGACGGTGATGACACGCATGCGATTGTGCTAGACAACATTAACCGCGATGAATTTGGTGATTATTTAAGTTTTTTAAACCTCGATGACGTGGTGTTTGAGGTTACTTTACCTACCAACCGTTCTGATTTACAGAGCTTAATCTTCCTCGCTAAGGAACTAGCAGCAGTGTTGAAACGTCCGGTCTTCTTGGAACAGAAAACCACCATGACCCTGCGCGAGTTCTACCGCTTTCCGCTTAACTTAAGAAACCGTGCTCAGGCCAATTTCTTTGGTGGACTTTTTTTGCGTGATGTAGCAATTACCAGTTCCCCTTGA", "aa": "MLISKKTLAVLIPEITHVSNNEICEKLQQIGIEVEAIRAFKNPDYLQLGILRAVTPHPHDNHLYVCQVQIDKNKQLNVVTGAVNIVDPNNLNKHVIVAKKGAELLNGLIIKTKNIKGIISDGMLCSYVDINPFSKHLIADGDDTHAIVLDNINRDEFGDYLSFLNLDDVVFEVTLPTNRSDLQSLIFLAKELAAVLKRPVFLEQKTTMTLREFYRFPLNLRNRAQANFFGGLFLRDVAITSSP", "aa_hexdigest": "618f93e3d9401336cac9c292fd75206e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01588.26", "name": "tRNA_bind", "length": 85, "aa_cov": 0.34156378600823045, "hmm_cov": 0.8823529411764706, "evalue": 3.160172227537711e-13, "score": 45.132057189941406, "start": 48, "stop": 130 } ], "seq_stats": { "molecular_weight": 27301.31710000002, "isoelectric_point": 6.653595161437989 }, "id": "APKECPOBEN_274", "locus": "GCA002090215_00237" }, { "type": "cds", "sequence": "contig_1", "start": 137481, "stop": 138140, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03483.22" ], "nt": "TTGTTAATTAACCAGGAGTTACGTCCAGTCAACTGCTTTGTGGACCAGGCTAATATGGTAACTGTTTACACTGGTCAACCAATCCACTGCCATGATGCTGACAAGATCCATGGAAGTGTGGAACTTAAACTAGCCACCCAATTGGAAACGATGTTGGCCTTAGACAACAAGGAGTATGAGATTAAACCGGGTGATCTAGTGGTAGCTGATGAACAGGGCACAATTGCCATAGTCGGTATTATTGGTAGTAAGCGCACTATGGTCGACAATACGACCAACAACATCTTTTTTGAAGTTGTTAACTATAACCATGAGCGAATTAAGCAAACCGCTCAGCGTTTAGGAGTTGCTAACTTTGCTAGTAGGTTAATGAGTAAACCAATTAGTTTACAAGCTACCGAAAACTGCTTGAACTATTTGCAAAATAACTTCCTCAATCCCGAAAGCATTGGCAAGATTAGTAAGTTTTCCAGCACCATTAAAGCCCCAGCTTTTAACCGCAAAATTTACCTCAACTTTAATCAGTTACGGGAGTTAATTGGAGTGACTAAAAAGCAGTTAAATGACCACATGATCCGTAACTACCTTACTAGTTTAGGGTTCAAAATGGAAAACCAAATTGCCCGCGCCCCCGCTTACCGGCAAGACATTACGGTTTGA", "aa": "MLINQELRPVNCFVDQANMVTVYTGQPIHCHDADKIHGSVELKLATQLETMLALDNKEYEIKPGDLVVADEQGTIAIVGIIGSKRTMVDNTTNNIFFEVVNYNHERIKQTAQRLGVANFASRLMSKPISLQATENCLNYLQNNFLNPESIGKISKFSSTIKAPAFNRKIYLNFNQLRELIGVTKKQLNDHMIRNYLTSLGFKMENQIARAPAYRQDITV", "aa_hexdigest": "d210209698909296250efceacd381e2a", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03483.22", "name": "B3_4", "length": 143, "aa_cov": 0.6529680365296804, "hmm_cov": 1.0, "evalue": 5.115426631092767e-44, "score": 145.37997436523438, "start": 2, "stop": 144 } ], "seq_stats": { "molecular_weight": 24805.24300000002, "isoelectric_point": 8.705504417419434 }, "id": "APKECPOBEN_275", "locus": "GCA002090215_00238" }, { "type": "cds", "sequence": "contig_1", "start": 138159, "stop": 138545, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF17759.6" ], "nt": "TTGTTAAAGATCTTGGACCTCAACAAGATTAAAGAGGATGAAATTTTAAGCTCCACTAAACTAGAGAAACACGAAAAACTCAATGCTTACGATGCGTTGCAAAAGCTACGCACTAAACTGCAAACCTTGGGCTTTCACAATGTGATTACTTACCAGTTAATTTCACCAGAACGAGCGCGTAACTTTAACCTGTTTGGGTTGAGTAATTTGTGGGAAATTAAAAACCCGCTTTCCAACGAACGGAGTGTTTTACGGGTTGGTTTAATTGACTCCTTGTTGCGAGTAATTCAAAAGAATGCCGCATACAAAAATAAGTTAGGCAACATCTTTGAGTTTTCTTTTGTCAAAACTAAGGACAGTAACCAACTCCACATTGCTGCTCTTTGA", "aa": "MLKILDLNKIKEDEILSSTKLEKHEKLNAYDALQKLRTKLQTLGFHNVITYQLISPERARNFNLFGLSNLWEIKNPLSNERSVLRVGLIDSLLRVIQKNAAYKNKLGNIFEFSFVKTKDSNQLHIAAL", "aa_hexdigest": "297dad79698a491f4bebb0f3bdb3401e", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF17759.6", "name": "tRNA_synthFbeta", "length": 112, "aa_cov": 0.8203125, "hmm_cov": 1.0, "evalue": 5.991995580878008e-20, "score": 67.0692138671875, "start": 17, "stop": 121 } ], "seq_stats": { "molecular_weight": 14795.048399999985, "isoelectric_point": 9.733388328552245 }, "id": "APKECPOBEN_276", "locus": "GCA002090215_00239" }, { "type": "cds", "sequence": "contig_1", "start": 138555, "stop": 139154, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF17759.6" ], "nt": "ATGTTTGGTTCTACCTACCAAAAGGATCAAGGCGTTAGTGTCGATATTCCCGCCATGAAGGGCTTAGCGCAGTTAATTATTTCCAACTTTGGTTTTAACTGCGACTTTGAACCAATTACTGAAGGTGAGTACTTCACTAAGAACGTCGGTTTAAAACTAGTGGTGTTTAACGAACAGATTGGTTATGTTGGTTTAATTAAGGATGAGCTGTTGGCGCCTTATGATTTAAAGGGTAGACCGGTGTATGGTTTGGAAATTAACCTAGACCGCTTGTTGAACTCCTTAAACCGGTTGGAGCGCAGTTATACCCCGATTAGCAAACTACAAGATGTCTTTAAGGATATTACCTTTAGCTTCCCCCGTGATGAAAGTCACTTTGAAACGTTTGTGAAAGCGATTAAAAAACTACAAACGATTTTTAAATGGGAATTGATCTCGGTGTTTGACACCGAAAAGGATGGTGTACCAATCACCAAATATACAGTGCGCTATTACTTAAAGAATTTCACTAATACACCATTAACGTTAGAACAAATTAAAGCAGTGGAAACGCAGTTAAAACAACAGTGTGAACTAGCTAAAATAGCACTCGATCTTTAG", "aa": "MFGSTYQKDQGVSVDIPAMKGLAQLIISNFGFNCDFEPITEGEYFTKNVGLKLVVFNEQIGYVGLIKDELLAPYDLKGRPVYGLEINLDRLLNSLNRLERSYTPISKLQDVFKDITFSFPRDESHFETFVKAIKKLQTIFKWELISVFDTEKDGVPITKYTVRYYLKNFTNTPLTLEQIKAVETQLKQQCELAKIALDL", "aa_hexdigest": "3080ec9fa8f0eeee8f5be8ddf20281b8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF17759.6", "name": "tRNA_synthFbeta", "length": 81, "aa_cov": 0.4020100502512563, "hmm_cov": 0.9753086419753086, "evalue": 3.487381639840426e-09, "score": 31.887662887573242, "start": 9, "stop": 88 } ], "seq_stats": { "molecular_weight": 22883.141000000014, "isoelectric_point": 5.5789403915405265 }, "id": "APKECPOBEN_277", "locus": "GCA002090215_00240" }, { "type": "cds", "sequence": "contig_1", "start": 139267, "stop": 140022, "strand": "+", "frame": 1, "gene": "ytxK", "product": "putative protein MPN_107", "db_xrefs": [ "COG:COG0827", "COG:L", "RefSeq:WP_010874464.1", "SO:0001217", "UniParc:UPI000013A1B8", "UniRef:UniRef100_P75562", "UniRef:UniRef50_P75562", "UniRef:UniRef90_P75562" ], "nt": "ATGACAAACCAAAACCTACAAGACTTTCTGGACGGAGTTTTGTTAAAAGAGGAAAATTTAAACAAAATTACTGAAGTTTCCCTAGAAAAGGGATTACCGCACCTTTTAGATGAGGGTATCCATAACGAAGATAACTTTCAGATCTTTACGAGCCAAAAGATTGTGGCAGACATGATCAAAATGGTCGGTGTAAAGGAAATGAAAAATGTCCACACTACTGTTTTAGAACCAACTAGTGGTGATGGTGCTTTTACTTGTGCTATTTTGGATTTACGTTTAAAAAACATTAAAAAATCAGACAGCTTTTTAAAAGAAGCTTTAACCGGTCTATCCACCATTTATTCAGTTGAAATTGACGAAGAGTTGGTTTGGTTACAACGCAATAACCTTTACTCTATCTTTTTAGCCTTCTTAAAAAAGCACGGTATTTTTAACGAAGAGTTAAGTCTCTTAGTGAAAAAAATCTTGATGATTAATGTGAGTTGGGGCGAGTTAATCACAGAAACCAAAGATAACGAGAACTTCGTTTGCAACTACGATTATTTTTTAAGGAATAAAAAGACAAAGAAAAAACCCGCAAAGCATAAACATGAATTTCCAGAGGGTTTATTTTCCAATGGAGAAGGTAAGTGCAAAAGTTTGTTTGTTAATTGGTCATTTGCTGATGTCATTAATGAAATTGCTCATAATTCCAATTTTTACTTTGCCAAAATAGCAAAGATACCTGGTGAAATAATTCAATTTCAAATTAATTAG", "aa": "MTNQNLQDFLDGVLLKEENLNKITEVSLEKGLPHLLDEGIHNEDNFQIFTSQKIVADMIKMVGVKEMKNVHTTVLEPTSGDGAFTCAILDLRLKNIKKSDSFLKEALTGLSTIYSVEIDEELVWLQRNNLYSIFLAFLKKHGIFNEELSLLVKKILMINVSWGELITETKDNENFVCNYDYFLRNKKTKKKPAKHKHEFPEGLFSNGEGKCKSLFVNWSFADVINEIAHNSNFYFAKIAKIPGEIIQFQIN", "aa_hexdigest": "30cae364e953bc25f85b3e4c5dfc0730", "start_type": "ATG", "rbs_motif": "AATAA", "ups": { "uniparc_id": "UPI000013A1B8", "ncbi_nrp_id": "WP_010874464.1", "uniref100_id": "UniRef100_P75562", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013A1B8", "RefSeq:WP_010874464.1", "UniRef:UniRef100_P75562" ] }, "ips": { "uniref100_id": "UniRef100_P75562", "uniref90_id": "UniRef90_P75562" }, "psc": { "uniref90_id": "UniRef90_P75562", "gene": "YtxK", "product": "Uncharacterized protein MPN_107", "uniref50_id": "UniRef50_P75562", "cog_id": "COG0827", "cog_category": "L" }, "pscc": { "uniref50_id": "UniRef50_P75562", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75562" ], "product": "Uncharacterized protein MPN_107" }, "genes": [ "ytxK" ], "id": "APKECPOBEN_278", "locus": "GCA002090215_00241" }, { "type": "cds", "sequence": "contig_1", "start": 140024, "stop": 140170, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTCAAAATGGCGATAAAGACCACGAATTGATTCAGGGAAAGGTCAAAAGCAAAACTGAAATAGAAGATTCCGTTATATTGGATCAAGACACTTTGCAAGGCAAGGGTGAAGAAAAAACTAAATTAGATGAGTTGATTAGGTGA", "aa": "MSQNGDKDHELIQGKVKSKTEIEDSVILDQDTLQGKGEEKTKLDELIR", "aa_hexdigest": "f3f5d9b52d27eb5d5157aa0bf9fa26b1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5440.999399999999, "isoelectric_point": 4.749886894226075 }, "id": "APKECPOBEN_279", "locus": "GCA002090215_00242" }, { "type": "cds", "sequence": "contig_1", "start": 140378, "stop": 140560, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGCCAAAGTTATACCGGATGAATATGAACGCAAGAAACACATCATGACCAAGATGCTTTTTAGTATTGACTTAACTAAATTTACTAGCTTGATGGTTCGTCGTACGCTTTACTATTGCATTAAAGCTAACAAGCGTAAAACTAGTGAAGATGAGGGCTGTGCAATTGCAAATGGCGCATGA", "aa": "MAKVIPDEYERKKHIMTKMLFSIDLTKFTSLMVRRTLYYCIKANKRKTSEDEGCAIANGA", "aa_hexdigest": "13a6ddf81ceaae4e007af63f4d0b1fd7", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6960.175300000001, "isoelectric_point": 9.468423271179201 }, "id": "APKECPOBEN_280", "locus": "GCA002090215_00243" }, { "type": "cds", "sequence": "contig_1", "start": 140529, "stop": 140720, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGGGCTGTGCAATTGCAAATGGCGCATGATTTAATAACGAATGCGGTAATGTTGTTAAACCTTACAAGGAACATTATTTCGACAAACAAGGAAAAAATAAAAAATGTAAGTTTTGTAGAACAGATGAGAAATTTAAATACCAACAATCTTCTTCAAATGAAAAATACGCCTATGACTTCATTCATTTAA", "aa": "MRAVQLQMAHDLITNAVMLLNLTRNIISTNKEKIKNVSFVEQMRNLNTNNLLQMKNTPMTSFI", "aa_hexdigest": "0fdad38dbf56bf509e89c74389e95626", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7290.559700000002, "isoelectric_point": 10.432676887512205 }, "id": "APKECPOBEN_281", "locus": "GCA002090215_00244" }, { "type": "cds", "sequence": "contig_1", "start": 140765, "stop": 140929, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF07669.17" ], "nt": "ATGAAATTTGATGTAATTATTGGTAATCCACCTTATCAGTTAGCAAATAATCGTGGTGGTGATGCTGGTGATGGTAATGGAGCAAACCCCATTTTTCAAGAATTTATCTTAAGAGCACTTGAATTACAACCAAAATATTTAGCAATGATTGTTCCTGCAAGATGA", "aa": "MKFDVIIGNPPYQLANNRGGDAGDGNGANPIFQEFILRALELQPKYLAMIVPAR", "aa_hexdigest": "0be93b2c684b66dce19192d4acd70eb0", "start_type": "ATG", "rbs_motif": "AAAA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07669.17", "name": "Eco57I", "length": 65, "aa_cov": 1.0, "hmm_cov": 1.0, "evalue": 1.7447457629135124e-14, "score": 49.69576644897461, "start": 1, "stop": 54 } ], "seq_stats": { "molecular_weight": 5915.756799999999, "isoelectric_point": 6.0008562088012685 }, "id": "APKECPOBEN_282", "locus": "GCA002090215_00245" }, { "type": "cds", "sequence": "contig_1", "start": 141614, "stop": 141811, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F2B7000", "UniRef:UniRef90_UPI00132F963A" ], "nt": "TTGTTGAACCTACTGGAGTTTGCACTCATAGTTATTGTAGTTGCTTATTTAACGACAAATAAGTTTGAAGTTGAAAATGTCTTTTTATACATGAAAACTAAATTTTTTAGATTTTTTGTCGAGATTTTTAAAGCAACCATCAATATCAGTTCACATAACTTCAAATACGTTCCCATTCAAGATTTTTCCCGTCCTTGA", "aa": "MLNLLEFALIVIVVAYLTTNKFEVENVFLYMKTKFFRFFVEIFKATINISSHNFKYVPIQDFSRP", "aa_hexdigest": "40d71a42dad9dab59fb3d80a7bdde41f", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 141559, "stop": 141898, "observations": { "insertion": [ 141649 ], "stop": [ 141809, 141857 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00132F963A", "query_cov": 0.8153846153846154, "subject_cov": 0.4732142857142857, "identity": 1.0, "score": 105.0, "evalue": 4.22e-28, "gene_start": 13, "gene_end": 65, "reference_start": 31, "reference_end": 83, "reference_sequence": "MPRITGSGVFGDYIPNIKIVEPTGVCTHSYVVAYLTTNKFEVENVFLYMKTKFFRFFVEIFKATINISSHNFKYVPIQDFSRPWNDRELYQKYNLTQEEWQYIENNISSYEN" }, "paralog": false, "identity": 0.9642857142857143, "score": 215.0, "evalue": 6.39e-74, "description": "Internal stop codon at 141809, 141857. Frameshift due to insertion around 141649.", "psc": { "uniref90_id": "UniRef90_UPI00132F963A", "uniref50_id": "UniRef50_UPI001F2B7000", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00132F963A", "UniRef:UniRef50_UPI001F2B7000" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001F2B7000", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F2B7000" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 7787.142499999996, "isoelectric_point": 9.157298851013184 }, "id": "APKECPOBEN_283", "locus": "GCA002090215_00246" }, { "type": "cds", "sequence": "contig_1", "start": 142337, "stop": 143677, "strand": "+", "frame": 2, "gene": null, "product": "DEAD/DEAH box helicase family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB33HLI1", "UniRef:UniRef90_UPI0036F40F46" ], "nt": "ATGAAAATAAGGATAAGTTTGAATATCTTAGCCAAATTTTTAGACAGTTTCGCGCACCTTCTCTTTTTAAAAAATAAGGAAATTTATACACCTAGAAAAGAGCAAGCCGCATGTGTTGAAGTGTTAGAAAGATACTTTCAAGCCAATCCCGAAAACGGTAGGTTTTTAATGAACTGCAAAATGCGGTTCGGTAAATGTTTCACCTTGTACTCTTATGCACAAAAGAATAACATTAACAAGATTTTGATCCTAACGTTTGTTCCAGCTGTCGAAGAATCTTTAAAGGATGATTTAAATCACATTGAAAAGAATTACAAATTTTATACCGATGATGATTTGCAAAAATCTAATTTTGATTTAAAGAATCAAAACGAGCCTTATGTAGTCTTTTTATCACTGCAAAACGTCTTAGGTAAACAAAGAATAGATGGCGCCAAAACTGATTTTGATAAAGAGCGAATTAGTAAGCTACAAGAGATTGATTTTGATTTAATTGTTTTTGATGAGTACCACTACGGAGCCAACAAAAAACGAACCCAAATCAAAGTTGAAAAAGTCAATAAAAAGATTGATAATCCAGAACAGCAAGATAATCAAGATGATGCTGAGGAAGAATTGGCAAGTACTTTTAAGTTAAAAGACATCAAAAGTAAATTTCAATTTTCATACAAACAATTAGTTTGTCTGTCAGGTACTCCTTTTTCCTCTTTAAGAAATAATGAGTTTTCCAGTAAAGATCAGGTGTTTACTTATTCTTACTTTGATGAACAGAAGGCCAAATCAGCAGAAAATCACCCACTCAAATTAGGGCAATACGGAATATTTCCGGAAATGAATATTTACTGTTTTGAATTGGCGGAAATTTTTACTGCACAAGAACAAGAAATATTCATAACTCCAGGTAAAGGTAAAAATAAGTTACCTGAAATTAGTTTTCGAAAGCTGTTTCAAACGGAAAACGTTTCTAAAGAATCAAGCAAACCAGTTTACAGATTTGTAAATGAAAACCTGGTTGAAAAGCTTATAGATTCTTTAATTGATAAACGTAAAGGTTTTAGCCACACACCTTTAAGTTGGGAAAACATTGACAAGCATAAGCATTCCTTATTAATTTTGCCAACTAGAGTTGCTTGTTTTGCGTTGGCCAATTTACTTAAAAATCACTGGTACTTTGAAAACAATGATTTTCAAATTATCAACATGTCAGAGAGCCAGTTTGGTAATGGCAAAAAAGCTTTAATTGAGCTTAATAAACACTTAGATGAAGCTAAAAAGACTAATAAAAATACTTTAACTATTACTGTAGCTAAATTAACTATTGGTATTACAGTTAAAGAGTGA", "aa": "MKIRISLNILAKFLDSFAHLLFLKNKEIYTPRKEQAACVEVLERYFQANPENGRFLMNCKMRFGKCFTLYSYAQKNNINKILILTFVPAVEESLKDDLNHIEKNYKFYTDDDLQKSNFDLKNQNEPYVVFLSLQNVLGKQRIDGAKTDFDKERISKLQEIDFDLIVFDEYHYGANKKRTQIKVEKVNKKIDNPEQQDNQDDAEEELASTFKLKDIKSKFQFSYKQLVCLSGTPFSSLRNNEFSSKDQVFTYSYFDEQKAKSAENHPLKLGQYGIFPEMNIYCFELAEIFTAQEQEIFITPGKGKNKLPEISFRKLFQTENVSKESSKPVYRFVNENLVEKLIDSLIDKRKGFSHTPLSWENIDKHKHSLLILPTRVACFALANLLKNHWYFENNDFQIINMSESQFGNGKKALIELNKHLDEAKKTNKNTLTITVAKLTIGITVKE", "aa_hexdigest": "fbb33ea7be90f4c02c50a6afa9a4c045", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0036F40F46", "query_cov": 0.9596412556053812, "subject_cov": 0.9953488372093023, "identity": 1.0, "score": 847.0, "evalue": 5.59e-310, "valid": true, "product": "DEAD/DEAH box helicase family protein", "uniref50_id": "UniRef50_A0AB33HLI1" }, "pscc": { "uniref50_id": "UniRef50_A0AB33HLI1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB33HLI1" ] }, "genes": [], "id": "APKECPOBEN_284", "locus": "GCA002090215_00247" }, { "type": "cds", "sequence": "contig_1", "start": 143693, "stop": 143881, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAGGATTTAAAGGGTGCTGAATCTTATTTCCAAACGATTTTTAGAATCCAAACTCCATATATAAAGAACTGTAAAAATCTGAAAGAAATTTGTTATGTTTACGACTTCAACATGTATAGGTGTTTGGAAGTTACTAACGAGTATTCAAAACAAACACAAACAGACCCTAAATTTAGTGCTAGTTGA", "aa": "MKDLKGAESYFQTIFRIQTPYIKNCKNLKEICYVYDFNMYRCLEVTNEYSKQTQTDPKFSAS", "aa_hexdigest": "793492b363d0808a3826746ea9fc44b2", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7435.424700000002, "isoelectric_point": 8.458783912658692 }, "id": "APKECPOBEN_285", "locus": "GCA002090215_00248" }, { "type": "cds", "sequence": "contig_1", "start": 144011, "stop": 144256, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATGAAAGTAACATTATCGACATAGATGTACTTTGCAATGTTGGGCAAGATGAAGATGCGCAAAAAATATTGAAAAAAATATTAGCTCATAAAAAATTTAAATCATCCAAAAAGAAGCGAGAGTTTGAAGACGTTCATTTGGAAAAAAGTCCAAAAAGTGAGGCATTTAGTGAAGGCGTTCGCTCTGGTAAGGATTACGCCGCTGAACAAGGTAATGTTTTAGAAGAACTTGAAAATTTCTGA", "aa": "MDESNIIDIDVLCNVGQDEDAQKILKKILAHKKFKSSKKKREFEDVHLEKSPKSEAFSEGVRSGKDYAAEQGNVLEELENF", "aa_hexdigest": "ffba8b18e2dc67ab777a0ede53ab4c82", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9226.243599999996, "isoelectric_point": 5.168903923034666 }, "id": "APKECPOBEN_286", "locus": "GCA002090215_00249" }, { "type": "cds", "sequence": "contig_1", "start": 144399, "stop": 144566, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTCAGAATAAAATCAAAGACTTTAAAAAAAGAAAAGGAAGAACAACTTCAACTTTTAAAAAATGAAAATGGTTATATTTCTGATGAGAATAACTTAAAAAAGCTAAGAGATTTAATTAAAGAATATGTACAAAACAGAAGCAACATACCTCCAGAATTAGACTGA", "aa": "MFRIKSKTLKKEKEEQLQLLKNENGYISDENNLKKLRDLIKEYVQNRSNIPPELD", "aa_hexdigest": "1eecdc65706310bc9296577b921531cf", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6633.5424, "isoelectric_point": 9.003348350524902 }, "id": "APKECPOBEN_287", "locus": "GCA002090215_00250" }, { "type": "cds", "sequence": "contig_1", "start": 144693, "stop": 144947, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB33HU34", "UniRef:UniRef90_A0AB33HU34" ], "nt": "ATGCATACATGGGCACGTGTTCTGCACTTTCTTTATGTATCTGTTTATAGAGAAAAAACTTTAAGAGATGTGTTGAATAAAACTGATCCCAAAGTTTTTTCAGTGGCAGTCGGAATTGAAATGGATGAGTTTTCCAAAATCCAAAAATTTTTTAAAGTGGAAAATATGAATAGACTCATCGAACAGTTTAGTTTTAGAGAAAGTGTTAAAACTGAAAACACAGAAAAATCACAGTATAGAAACAGTTTCAATTGA", "aa": "MHTWARVLHFLYVSVYREKTLRDVLNKTDPKVFSVAVGIEMDEFSKIQKFFKVENMNRLIEQFSFRESVKTENTEKSQYRNSFN", "aa_hexdigest": "e12e6933ae29fb8dd708477d305bc870", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 144399, "stop": 144980, "observations": { "mutation": [ 144564, 144945 ], "stop": [ 144564, 144945 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB33HU34", "query_cov": 1.0, "subject_cov": 0.43523316062176165, "identity": 1.0, "score": 166.0, "evalue": 9.79e-51, "gene_start": 1, "gene_end": 84, "reference_start": 99, "reference_end": 182, "reference_sequence": "LFRIISKTLKKEKEEQLQLLKNENGYISDENNLKKLRDLIKEYVQNRSNIPPELDWFFYRDRFIQLFEKSCYQHFKKKTLTEEQKEKKKKVNDNFDSLMHTWARVLHFLYVSVYREKTLRDVLNKTDPKVFSVAVGIEMDEFSKIQKFFKVENMNRLIEQFSFRESVKTENTEKSQYRNSFNWFGFGPDSLKS" }, "paralog": false, "identity": 0.9844559585492227, "score": 379.0, "evalue": 6.79e-137, "description": "Internal stop codon at 144564, 144945. Nonsense mutation around 144564, 144945.", "psc": { "uniref90_id": "UniRef90_A0AB33HU34", "uniref50_id": "UniRef50_A0AB33HU34", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB33HU34", "UniRef:UniRef50_A0AB33HU34" ] }, "pscc": { "uniref50_id": "UniRef50_A0AB33HU34", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB33HU34" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 10157.494499999995, "isoelectric_point": 9.344708442687988 }, "id": "APKECPOBEN_288", "locus": "GCA002090215_00251" }, { "type": "cds", "sequence": "contig_1", "start": 144997, "stop": 145143, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACAAAAATAGTGGTAAAGACTACGAATCGATTCAAGGTAAGATTAAGAGTAAAGCTGAAATAGAAGATTCAGTTATATTGGAAAAAGAAACTTTTCAAGGCAAGGGTGAAGAAAAAACTAAATTAGATGAGTTGATTAGGTGA", "aa": "MNKNSGKDYESIQGKIKSKAEIEDSVILEKETFQGKGEEKTKLDELIR", "aa_hexdigest": "71589bd9f02035b41ee0f78b523ad5ac", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5486.124399999999, "isoelectric_point": 5.364145851135253 }, "id": "APKECPOBEN_289", "locus": "GCA002090215_00252" }, { "type": "cds", "sequence": "contig_1", "start": 145396, "stop": 145893, "strand": "+", "frame": 1, "gene": null, "product": "Eco57I restriction-modification methylase domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00132FCD8E", "UniRef:UniRef90_UPI00133023F0" ], "nt": "ATGACCAAGATGCTTTTTAGCATTGGCTTAACTAAATTTACTAGTTTGATGGTGCGTCGCACGCTTTACTATTGCATTAAAGCTAACAAGCGTAAAACCAGCGAAGATGAGGGTTGTGCAATTGCAAATGGCGCACGATTTAATAATGAATTTGGCAATGTTGTTACCCCTTATAAAGAACACTATTTTGGAAAAGAATCGAAAAGCAAAAACTGTCACTTTTGTGGGACAAATAAGAATTCTAAATATGTAAATTCTATGACTGAAGAAAAGTATGCCTATGACTTCATTCATTTAAATTCGGATGAATATGAAAACTACTTTAAAACTAATTTTGGTGTTATGAAATTTGATGTAATTATTGGAAATCCGCCTTACCAGCTATCTAATGGTAGCGGTTCTGATGGCAATGGAGCCAAAGCAATTTTTCAAGATTTTGTTTTAAAAGCAATTGACTTAGAACCAAAATATTTAGCGATGATTATCCCAGCAAAATGA", "aa": "MTKMLFSIGLTKFTSLMVRRTLYYCIKANKRKTSEDEGCAIANGARFNNEFGNVVTPYKEHYFGKESKSKNCHFCGTNKNSKYVNSMTEEKYAYDFIHLNSDEYENYFKTNFGVMKFDVIIGNPPYQLSNGSGSDGNGAKAIFQDFVLKAIDLEPKYLAMIIPAK", "aa_hexdigest": "db4ec91516e2b2abcd723e7e7805bcfc", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 145096, "stop": 145931, "observations": { "deletion": [ 145909 ], "mutation": [ 145141, 145264, 145285, 145891 ], "stop": [ 145141, 145264, 145285, 145891 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00133023F0", "query_cov": 1.0, "subject_cov": 0.532258064516129, "identity": 1.0, "score": 337.0, "evalue": 5.44e-115, "gene_start": 1, "gene_end": 165, "reference_start": 134, "reference_end": 298, "reference_sequence": "MNKNSGKDYESIQGKIKSKAEIEDSVILEKETFQGKGEEKTKLDELIRWEAEKNDLLKLIDNVGNDEVFTPVETCQRMLDELFPEDHEVWSNPDLKWLNPCDKNGVFFREIALRLDKGLTKIIPDEYARKKHIMTKMLFSIGLTKFTSLMVRRTLYYCIKANKRKTSEDEGCAIANGARFNNEFGNVVTPYKEHYFGKESKSKNCHFCGTNKNSKYVNSMTEEKYAYDFIHLNSDEYENYFKTNFGVMKFDVIIGNPPYQLSNGSGSDGNGAKAIFQDFVLKAIDLEPKYLAMIIPAKWMISAKYFFKSK" }, "paralog": false, "identity": 0.9820143884892086, "score": 544.0, "evalue": 3.64e-199, "description": "Internal stop codon at 145141, 145264, 145285, 145891. Frameshift due to deletion around 145909. Nonsense mutation around 145141, 145264, 145285, 145891.", "psc": { "uniref90_id": "UniRef90_UPI00133023F0", "product": "Eco57I restriction-modification methylase domain-containing protein", "uniref50_id": "UniRef50_UPI00132FCD8E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00133023F0", "UniRef:UniRef50_UPI00132FCD8E" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00132FCD8E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00132FCD8E" ], "product": "Eco57I restriction-modification methylase domain-containing protein" } }, "id": "APKECPOBEN_290", "locus": "GCA002090215_00253" }, { "type": "cds", "sequence": "contig_1", "start": 145894, "stop": 146181, "strand": "+", "frame": 1, "gene": null, "product": "Eco57I restriction-modification methylase domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F3844D4", "UniRef:UniRef90_UPI001F3844D4" ], "nt": "ATGATTTCTGCTGAAAATATTTTTTTAAATCTAAGAGATAAATTAAAGAAAAATAAAGATATAAAAGAAATTAATATTTTTTTCGACTCTAAAGACTGTTTTCCAAAGAGAGAAATAAAAGGTGGCATTTGTTACTTTATCTGGCAGAATAACTATCAAGGTAAGACTCTTATAAATACCAAATTTTCAAAGAAAGGAAAAAGCACAGAGATCGATAGTTCTAAAAGAAACTTATTTGTTTCCGTTTTAAACAACACTGAACAAATCATTTTTCGCAAAAGGATTTGA", "aa": "MISAENIFLNLRDKLKKNKDIKEINIFFDSKDCFPKREIKGGICYFIWQNNYQGKTLINTKFSKKGKSTEIDSSKRNLFVSVLNNTEQIIFRKRI", "aa_hexdigest": "226394824360c194701a68383e255bbc", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 145909, "stop": 146265, "observations": { "mutation": [ 146179 ], "stop": [ 146179 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001F3844D4", "query_cov": 0.9473684210526315, "subject_cov": 0.7627118644067796, "identity": 0.9890000000000001, "score": 180.0, "evalue": 3.39e-57, "gene_start": 6, "gene_end": 95, "reference_start": 1, "reference_end": 90, "reference_sequence": "NNFLNLRDKLKKNKDIKEINIFFDSKDCFPKREIKGGICYFIWQNNYQGKTLINTKFSKKGKSTEIDSSKRNLFVSVLNNTEQIIFRKRIWQDIYTKIDSISASEMKNTHTEREQFRS" }, "paralog": false, "identity": 0.9830508474576272, "score": 234.0, "evalue": 1.14e-80, "description": "Internal stop codon at 146179. Nonsense mutation around 146179.", "psc": { "uniref90_id": "UniRef90_UPI001F3844D4", "product": "Eco57I restriction-modification methylase domain-containing protein", "uniref50_id": "UniRef50_UPI001F3844D4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001F3844D4", "UniRef:UniRef50_UPI001F3844D4" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001F3844D4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F3844D4" ], "product": "Eco57I restriction-modification methylase domain-containing protein" } }, "id": "APKECPOBEN_291", "locus": "GCA002090215_00254" }, { "type": "cds", "sequence": "contig_1", "start": 146348, "stop": 146818, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_026088310.1", "SO:0001217", "UniParc:UPI0004755848", "UniRef:UniRef100_A0AB38WA20", "UniRef:UniRef50_A0AB38WA20", "UniRef:UniRef90_A0AB38WA20" ], "nt": "TTGAAAGCAGAAAAATTTCCCGGTTTGATTGCAATTTATGGAAGTCATTCGGTTATTAAACACGTGGATATTAAAGATATTCCGCAAATAAAAAATAATGAAGAAATTGCTAAATACAAAATTATTGTTCCACACGTTTATAGCCGTGGCAATGGAACTTTTGGAAATTACAAACCAAAAGTAAAAATTATTAAACCTAATGAAATTTGCACCGAAACTTATTTAGTGGTATATCCCACCGAAAGTAAAGTTGAGATTGAAAACGTCGCTTCTTATATGAGAACTAAATTTTTCAGATTTTTAGTTGAAATTTTTAAGGATTCTATTAACACAAATTCTCAAAATTTCAAGTTTATTCCTCTACAAGATTTTTCACGTCCTTGGAACGATAGAGAACTTTATGAAAAATACGGTTTAACACTCGAAGAACAGCAATACATCGAGGCGAACATAAGTGCTTACGAGGACTAA", "aa": "MKAEKFPGLIAIYGSHSVIKHVDIKDIPQIKNNEEIAKYKIIVPHVYSRGNGTFGNYKPKVKIIKPNEICTETYLVVYPTESKVEIENVASYMRTKFFRFLVEIFKDSINTNSQNFKFIPLQDFSRPWNDRELYEKYGLTLEEQQYIEANISAYED", "aa_hexdigest": "c0716801b80d9d4d8bec1f38971cef67", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0004755848", "ncbi_nrp_id": "WP_026088310.1", "uniref100_id": "UniRef100_A0AB38WA20", "db_xrefs": [ "SO:0001217", "UniParc:UPI0004755848", "RefSeq:WP_026088310.1", "UniRef:UniRef100_A0AB38WA20" ] }, "ips": { "uniref100_id": "UniRef100_A0AB38WA20", "uniref90_id": "UniRef90_A0AB38WA20" }, "psc": { "uniref90_id": "UniRef90_A0AB38WA20", "uniref50_id": "UniRef50_A0AB38WA20" }, "pscc": { "uniref50_id": "UniRef50_A0AB38WA20", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38WA20" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18312.711499999994, "isoelectric_point": 6.843323707580566 }, "id": "APKECPOBEN_292", "locus": "GCA002090215_00255" }, { "type": "cds", "sequence": "contig_1", "start": 147125, "stop": 147517, "strand": "+", "frame": 2, "gene": null, "product": "putative protein MPN_112", "db_xrefs": [ "GO:0005886", "RefSeq:WP_010874469.1", "SO:0001217", "UniParc:UPI000013A1CB", "UniRef:UniRef100_P75450", "UniRef:UniRef50_P75450", "UniRef:UniRef90_P75450" ], "nt": "ATGCTAGATAAATTACTCCAGAAATTTCGAGACCAAAAGAAGCCCGTCTTCCACAAAGAAGAGGGCTACTGGGAAATTAGTGCCTTGCGGAAATGGGCAGCCATTTTAATAATTGCCTTTGGAGCTGGCATTATTTACATTGTGCCCTACTTTGCCTTCTTTCAGTTTAAGACAGCAGTAGCTAATGTAACGGGAGTGGAGCCCAACAGAATCTCCCTTCTCTTAACTGCTTACGGGATTGTTTCATTGCTGTTTTATATTCCTGGTGGTTGGTTAGCTGACCGGATTTCAGCTAAAGCGCTGTTTTCGGTATCAATGTTTGGTACTGGCATTATTACCTTCTGGTACTTCTTAGTTGGTTTGAAGGGCATAGTGTGGATTACACCCAACTAG", "aa": "MLDKLLQKFRDQKKPVFHKEEGYWEISALRKWAAILIIAFGAGIIYIVPYFAFFQFKTAVANVTGVEPNRISLLLTAYGIVSLLFYIPGGWLADRISAKALFSVSMFGTGIITFWYFLVGLKGIVWITPN", "aa_hexdigest": "09afb655a9cf37dc9cec4897c8f01479", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013A1CB", "ncbi_nrp_id": "WP_010874469.1", "uniref100_id": "UniRef100_P75450", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013A1CB", "RefSeq:WP_010874469.1", "UniRef:UniRef100_P75450" ] }, "ips": { "uniref100_id": "UniRef100_P75450", "uniref90_id": "UniRef90_P75450" }, "psc": { "uniref90_id": "UniRef90_P75450", "product": "Uncharacterized protein MPN_112", "uniref50_id": "UniRef50_P75450", "go_ids": [ "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75450", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75450" ], "product": "Uncharacterized protein MPN_112" }, "genes": [], "id": "APKECPOBEN_293", "locus": "GCA002090215_00256" }, { "type": "cds", "sequence": "contig_1", "start": 147499, "stop": 147987, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGATTACACCCAACTAGTTATTATCCACGTCCTGTATGCCTTTAGTACGGTCTTAACCTTCTGGAGTGCCTTCATTAAAAGTATTAAGTTATTGGGTCCAGCGGATGAACAAGCGAGGTTGTATTCCACATCTGAAGGATCACGCTACTTAATCCAAGTAATTACTGGTTACATTAGTGCTGGTTTGGGTGCTATTGTCCTTATTAGTACTCCCTTTCTCATTTACAAAGCACCAACTGCTGCTGACCCTAAAAACTTAGCTTCTTCCTCAGGGATCTTCTTTGCCTTACTGTTTTATGCCATTTGTTATGTGTTAGCTGGTTTACTGTCACTGTTTGTTTTACCAGGAAAGTTCTGGGAAAAGCGCAAGATTTACTTGGAAGATGAACGAATTGAATACTTTAACCCTGTGGACCAGGCTTATCACATCTTTAAATCGGAAGCAGAATTAGAAGCCTTTAAGAAACATAACACTAAGGTTTTTTGA", "aa": "MDYTQLVIIHVLYAFSTVLTFWSAFIKSIKLLGPADEQARLYSTSEGSRYLIQVITGYISAGLGAIVLISTPFLIYKAPTAADPKNLASSSGIFFALLFYAICYVLAGLLSLFVLPGKFWEKRKIYLEDERIEYFNPVDQAYHIFKSEAELEAFKKHNTKVF", "aa_hexdigest": "47bbaebd9abd6fbff6716384a52a23bc", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18341.16129999999, "isoelectric_point": 7.786636543273926 }, "id": "APKECPOBEN_294", "locus": "GCA002090215_00257" }, { "type": "cds", "sequence": "contig_1", "start": 148066, "stop": 148350, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGTGTACCAAACGATTGCTAACTTTGGTACTAACATTTTGGAATCGCTAGGACTAGAGGCAGCACTCTCCCCAACTTTAAGTAATACCTATATCTATGGGATTCCGGTGTTAGGGGCAATCTTTTCTGGTTTGATTACCAAAAAGTTAACTAAATCAACCGCTAAAAGTCTGATTGTGCAAGCCTTGTTTGTCATTTTGGGTACCTTAGCCTTTTTAGTAATAACACTAGCTTCCCCAGCCAAACCAGAAACTGGTGCATTTAACCAAGCTACCTTGTGA", "aa": "MNVYQTIANFGTNILESLGLEAALSPTLSNTYIYGIPVLGAIFSGLITKKLTKSTAKSLIVQALFVILGTLAFLVITLASPAKPETGAFNQATL", "aa_hexdigest": "8ff8472e737886af9a0055f375aa799e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9860.502199999988, "isoelectric_point": 9.158330345153807 }, "id": "APKECPOBEN_295", "locus": "GCA002090215_00258" }, { "type": "cds", "sequence": "contig_1", "start": 148378, "stop": 148596, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTCTTTATTGGCGCGAACCGTTCCATTTTTTGGAGTACGATCACAGAACTGAAAGTCAATAAGGAAATAGTAGGACTGGCAGTTGGTTTTATTTCGATTATTGGTTTTTCCAAAGATGTGTGGTTAAGTCCCTTACTGACTGGTACTACTAACCAGTTCATTGTCAAAAACAGTCAGGGTACCTCGTTTTACAGCCAGCAAGCCTTGGTAGCCTGA", "aa": "MFFIGANRSIFWSTITELKVNKEIVGLAVGFISIIGFSKDVWLSPLLTGTTNQFIVKNSQGTSFYSQQALVA", "aa_hexdigest": "7ad1448c6ae1df7dfa260762d181f4f9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7932.1071999999995, "isoelectric_point": 9.398732948303223 }, "id": "APKECPOBEN_296", "locus": "GCA002090215_00259" }, { "type": "cds", "sequence": "contig_1", "start": 148727, "stop": 148876, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGACACTAAATTAACACCACCCTGGTTGCGTCAAGAAAATAACCTGCTCAACCCCGAAGTTACCCAACGTTTGTTTATCAACCAGGACAACATTCCGAACTTACCCACTGAAGCCCCCCAAGTTTATTTAGCCCGTTTTTTGGAATGA", "aa": "MDTKLTPPWLRQENNLLNPEVTQRLFINQDNIPNLPTEAPQVYLARFLE", "aa_hexdigest": "d35db3a995426340778277bb19f514a3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5792.5352, "isoelectric_point": 4.800644111633301 }, "id": "APKECPOBEN_297", "locus": "GCA002090215_00260" }, { "type": "cds", "sequence": "contig_1", "start": 148907, "stop": 149110, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAAAGCACAAGCAGCAGTTCAGGATTACCTCAATTCTAAAGCCTGTGCACAAATTGAAACCATTATTGCAGAACGGGCGCAAAACACCCATTCGAGTTGGTTAGCTAACTGGTGGGTGCAGTACGCTTATTTAACTTCGACTGGCCCGGTTAGTCCGGAAGTTAATGCACCCTATTATTTAGAGCTCCCGACTGTTGGTTGA", "aa": "MKAQAAVQDYLNSKACAQIETIIAERAQNTHSSWLANWWVQYAYLTSTGPVSPEVNAPYYLELPTVG", "aa_hexdigest": "b09559ff02a9e4e227171cbef723aa7f", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7460.283500000001, "isoelectric_point": 4.957690238952636 }, "id": "APKECPOBEN_298", "locus": "GCA002090215_00261" }, { "type": "cds", "sequence": "contig_1", "start": 149228, "stop": 149503, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAATCAATCTTTGCTAGTTGCCAGATTCACCGCGCTGATGGGGATGTGTACTTTGTTAATGATCAACCTGCTAACTTTATTGTGGTGATTAAGAACAATGTCTTCTATAAGTTAGTAATAGATAACAGTGGTAGCTTAGAACAACTGCAAGCGCAACTGCAGTTAAGCTTTGTGCAAATCTTAGACAATGAGCTAAGTCACCCACCGCACTGGAACTTACTCACTGCTACCACAACCAAAGCGGAGTCACAAAGTTTACTCGATCAGTTGTGA", "aa": "MQSIFASCQIHRADGDVYFVNDQPANFIVVIKNNVFYKLVIDNSGSLEQLQAQLQLSFVQILDNELSHPPHWNLLTATTTKAESQSLLDQL", "aa_hexdigest": "e3f5d7731d015054c906275db0fc817e", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10274.47999999999, "isoelectric_point": 4.721694755554199 }, "id": "APKECPOBEN_299", "locus": "GCA002090215_00262" }, { "type": "cds", "sequence": "contig_1", "start": 149519, "stop": 149611, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGTTGGACATCTATAACAGTGCCTTTATTGTGAACTTGGACAACGTCGAATTAACGACTCCCTTACAACTGTTGCGCAATTCGACATGA", "aa": "MLLDIYNSAFIVNLDNVELTTPLQLLRNST", "aa_hexdigest": "c17b110897154ee35c51f32e14948af8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3421.9122000000007, "isoelectric_point": 4.0500284194946286 }, "id": "APKECPOBEN_300", "locus": "GCA002090215_00263" }, { "type": "cds", "sequence": "contig_1", "start": 149651, "stop": 150301, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTCATTACTAAGAACGCTCAGTTAGTCATTCTAGCGGATCACACTAGTTTTGACGGTTCTAGTGTAGCTACCCTAGCCAACATCTTTGTCTCTAAACTCCAAAAGGTCAACACTGAGGGTGCCAGCGCTTTAACACCGACCATGCTTTCCTTTCCAACAGTAGACCAGGATAAGCAAAAGTATTTTAAGCAATTGAGCAAAAATTTTAAGGACTATGTGTACAATGCCGTTATGTTTGAACTGAAATGGGACTGGTTTACGAAGCCACTTATCAAAGCCAAAGGGATTAAGAACTCCGAAGCCTTTATCCATCTCTGTTACCAAATTGCCCAGTACCAAACGAACAAAAAGTTGCAGAACACCTATGTGGCCGTGGACATGCGTCAGTACTTCCGGGGACGCACCGAGTGTCTTCGTCCTTTAAGTAAACAATCAGTTGCCTTTGTCAAACGCTACTGCAAAGATCCGAAAGGTACCTTAAAGCAGTTCCGAAAGTACTACCCAGCGATTGAAAGTCTCCACTTTGAAAAAACCCGGTTAGCGCAAAAGGGTAGTGGGGTCAACCGTCACTTGCTGGGAGCATATTTGGCTTGGAATGAACACCAAGACACAATAGCTAAACCCGCTTTATTTGAAACAAAGGCTTGA", "aa": "MVITKNAQLVILADHTSFDGSSVATLANIFVSKLQKVNTEGASALTPTMLSFPTVDQDKQKYFKQLSKNFKDYVYNAVMFELKWDWFTKPLIKAKGIKNSEAFIHLCYQIAQYQTNKKLQNTYVAVDMRQYFRGRTECLRPLSKQSVAFVKRYCKDPKGTLKQFRKYYPAIESLHFEKTRLAQKGSGVNRHLLGAYLAWNEHQDTIAKPALFETKA", "aa_hexdigest": "d7166ce915cb106519eeea40626def43", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 24880.50170000001, "isoelectric_point": 9.722557640075681 }, "id": "APKECPOBEN_301", "locus": "GCA002090215_00264" }, { "type": "cds", "sequence": "contig_1", "start": 150341, "stop": 150529, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGACAAGTATTTGCGCAACTTTTCCTTCGACCCCGTAGAGCCAAACGGCATTGGTATTGCCTATGCCATTGATGACACTAATTTCCGTGCCATTCTCAGTGTGTATCAGCACAACTTACAGTACCTCAAAGATTGGATGAAACACTTTGAACAAACAGTGAAAACAATCCTTAAAACTCTTAAATAA", "aa": "MDKYLRNFSFDPVEPNGIGIAYAIDDTNFRAILSVYQHNLQYLKDWMKHFEQTVKTILKTLK", "aa_hexdigest": "84e55af061b4a9a5d65ce1d90d3f824d", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7380.4155, "isoelectric_point": 8.025427436828615 }, "id": "APKECPOBEN_302", "locus": "GCA002090215_00265" }, { "type": "cds", "sequence": "contig_1", "start": 150619, "stop": 151035, "strand": "+", "frame": 1, "gene": "infC", "product": "translation initiation factor IF-3", "db_xrefs": [ "COG:COG0290", "COG:J", "GO:0003743", "GO:0005829", "GO:0006413", "GO:0016020", "GO:0032790", "GO:0043022", "SO:0001217", "UniRef:UniRef50_P47438", "UniRef:UniRef90_P78024" ], "nt": "ATGCCGTTGATTCTTGATCAGGGCTGTTTTGTTAGTGAAAGTAGATTTCATAGATTGGCACAGAACATAAAACAAGGTTCCAGGCGTGAGCGCGAACAAAAACCGTTAATTAACGATAAAATTGGCTTTAATGAGTTCATTTTAATTGACGAAAATGGCTCCAATTTGGGGACGGTTAGAAGGACCGATGCCCTCAAGATGGCTGAGGAAAAACAACTCGATTTAGTCTTAATTGGTTCAAACCCAGCCAAACCCATTGTTAAGCTCTTAGACTTTGGTCGTTACACTTATGATTTAAAACGCAAAAAACGCCAGTCCAAGAAAAACCAAACCATTATCCAAATAAAGGAAGTGGTAGTGAAACCGACAATCGCTAAACATGATCTGGAATTTAAAGCTAAGCAAACGACGGGATGA", "aa": "MPLILDQGCFVSESRFHRLAQNIKQGSRREREQKPLINDKIGFNEFILIDENGSNLGTVRRTDALKMAEEKQLDLVLIGSNPAKPIVKLLDFGRYTYDLKRKKRQSKKNQTIIQIKEVVVKPTIAKHDLEFKAKQTTG", "aa_hexdigest": "9f687e2474f49e8dd47239253ae7c8a5", "start_type": "ATG", "rbs_motif": null, "genes": [ "infC" ], "truncated": "3-prime", "pseudogene": { "start": 150619, "stop": 151224, "observations": { "mutation": [ 151033 ], "stop": [ 151033 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P78024", "query_cov": 1.0, "subject_cov": 0.6865671641791045, "identity": 1.0, "score": 267.0, "evalue": 1.59e-89, "gene_start": 1, "gene_end": 138, "reference_start": 1, "reference_end": 138, "reference_sequence": "MPLILDQGCFVSESRFHRLAQNIKQGSRREREQKPLINDKIGFNEFILIDENGSNLGTVRRTDALKMAEEKQLDLVLIGSNPAKPIVKLLDFGRYTYDLKRKKRQSKKNQTIIQIKEVVVKPTIAKHDLEFKAKQTTGWAEKGYHVKFVVRAFGRVSTRIELIEKVFNDFYLLVEPAVEVQKPLTASSKTMYSALLVPRKK" }, "paralog": false, "identity": 0.9950248756218906, "score": 390.0, "evalue": 2.71e-140, "description": "Internal stop codon at 151033. Nonsense mutation around 151033.", "psc": { "uniref90_id": "UniRef90_P78024", "gene": "infC", "product": "translation initiation factor IF-3", "uniref50_id": "UniRef50_P47438", "cog_id": "COG0290", "cog_category": "J", "go_ids": [ "GO:0003743", "GO:0005829", "GO:0006413", "GO:0016020", "GO:0032790", "GO:0043022" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P78024", "UniRef:UniRef50_P47438", "COG:COG0290", "COG:J", "GO:0003743", "GO:0005829", "GO:0006413", "GO:0016020", "GO:0032790", "GO:0043022" ] }, "pscc": { "uniref50_id": "UniRef50_P47438", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47438" ], "product": "Translation initiation factor IF-3" } }, "id": "APKECPOBEN_303", "locus": "GCA002090215_00266" }, { "type": "cds", "sequence": "contig_1", "start": 151066, "stop": 151224, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCGCGCTTTTGGTAGAGTAAGTACTAGGATTGAGTTAATTGAAAAAGTCTTTAATGACTTTTATCTGCTAGTAGAACCAGCAGTGGAGGTCCAAAAACCACTAACTGCTTCTTCCAAAACAATGTATTCTGCTTTGTTAGTTCCCCGTAAGAAATAA", "aa": "MRAFGRVSTRIELIEKVFNDFYLLVEPAVEVQKPLTASSKTMYSALLVPRKK", "aa_hexdigest": "e9405b642686b6f7dd7b879505ab907d", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5972.030800000001, "isoelectric_point": 9.867998313903808 }, "id": "APKECPOBEN_304", "locus": "GCA002090215_00267" }, { "type": "cds", "sequence": "contig_1", "start": 151230, "stop": 151409, "strand": "+", "frame": 3, "gene": "rpmI", "product": "50S ribosomal protein L35", "db_xrefs": [ "COG:COG0291", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625", "KEGG:K02916", "RefSeq:WP_010874473.1", "SO:0001217", "UniParc:UPI0000133FF9", "UniRef:UniRef100_P75447", "UniRef:UniRef50_P75447", "UniRef:UniRef90_P75447" ], "nt": "ATGAAAGTAAAAAGTGCTGCAAAGAAGCGTTTTAAGCTCACTAAGTCTGGCCAAATTAAGCGCAAACACGCTTATACTTCCCACTTAGCACCGCACAAAACGACGAAGCAAAAGCGCCACTTGCGCAAGCAAGGAACTGTCAGTGCGAGTGACTTTAAACGCATTGGTAATTTAATTTAA", "aa": "MKVKSAAKKRFKLTKSGQIKRKHAYTSHLAPHKTTKQKRHLRKQGTVSASDFKRIGNLI", "aa_hexdigest": "5ed192a5600e39cb80ef8f07a769af7e", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000133FF9", "ncbi_nrp_id": "WP_010874473.1", "uniref100_id": "UniRef100_P75447", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000133FF9", "RefSeq:WP_010874473.1", "UniRef:UniRef100_P75447" ] }, "ips": { "uniref100_id": "UniRef100_P75447", "uniref90_id": "UniRef90_P75447" }, "psc": { "uniref90_id": "UniRef90_P75447", "gene": "rpmI", "product": "50S ribosomal protein L35", "uniref50_id": "UniRef50_P75447", "cog_id": "COG0291", "cog_category": "J", "kegg_orthology_id": "K02916", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_P75447", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75447" ], "product": "Large ribosomal subunit protein bL35" }, "genes": [ "rpmI" ], "id": "APKECPOBEN_305", "locus": "GCA002090215_00268" }, { "type": "cds", "sequence": "contig_1", "start": 151420, "stop": 151803, "strand": "+", "frame": 1, "gene": "rplT", "product": "50S ribosomal protein L20", "db_xrefs": [ "COG:COG0292", "COG:J", "GO:0000027", "GO:0000315", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022625", "GO:1990904", "RefSeq:WP_010874474.1", "SO:0001217", "UniParc:UPI0000133D19", "UniRef:UniRef100_P78023", "UniRef:UniRef50_Q7NBC0", "UniRef:UniRef90_P78023" ], "nt": "ATGAGAATTAAAGGTGGAAAACAAACCCGCGTACGTCGCAAGAAGTGGTTAAAGCAAGCTAGTGGTAGCTTTGGTACCCGACATGCCTCTTATAAGGTGGCAAAGCAAACGGTGATCCAAGCCGCTAAGTATGCTTACCGCGATCGTCGTAACAAAAAGCGCGATTTCCGTTCGTTGTGGATCTTGCGCTTAAACGCCGCTTTGCGCGAACAGGGCATGACCTATTCGGTCTTTATCAATCTATTGAAGAAGCACAACATTGAGATTAACCGCAAAGTGTTGTCGGAACTAGCCATTAAAGAACCAAGTAAATTTAACCTAATTGTGCAAAAGGTTAAAAGTGAACAACCGAAAGCCGCTAAGCCAGCTGCTTTAGGAAACTAG", "aa": "MRIKGGKQTRVRRKKWLKQASGSFGTRHASYKVAKQTVIQAAKYAYRDRRNKKRDFRSLWILRLNAALREQGMTYSVFINLLKKHNIEINRKVLSELAIKEPSKFNLIVQKVKSEQPKAAKPAALGN", "aa_hexdigest": "035eea5ede4fafe36c06d492b11e94cb", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000133D19", "ncbi_nrp_id": "WP_010874474.1", "uniref100_id": "UniRef100_P78023", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000133D19", "RefSeq:WP_010874474.1", "UniRef:UniRef100_P78023" ] }, "ips": { "uniref100_id": "UniRef100_P78023", "uniref90_id": "UniRef90_P78023" }, "psc": { "uniref90_id": "UniRef90_P78023", "gene": "rplT", "product": "50S ribosomal protein L20", "uniref50_id": "UniRef50_Q7NBC0", "cog_id": "COG0292", "cog_category": "J", "go_ids": [ "GO:0000027", "GO:0000315", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022625", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_Q7NBC0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q7NBC0" ], "product": "Large ribosomal subunit protein bL20" }, "genes": [ "rplT" ], "id": "APKECPOBEN_306", "locus": "GCA002090215_00269" }, { "type": "cds", "sequence": "contig_1", "start": 151778, "stop": 152488, "strand": "-", "frame": 3, "gene": "rnhC", "product": "Ribonuclease HIII", "db_xrefs": [ "COG:COG1039", "COG:L", "EC:3.1.26.4", "GO:0003723", "GO:0004523", "GO:0005737", "GO:0006298", "GO:0032299", "GO:0043137", "GO:0046872", "RefSeq:WP_010874475.1", "SO:0001217", "UniParc:UPI0000139442", "UniRef:UniRef100_P75446", "UniRef:UniRef50_P75446", "UniRef:UniRef90_P75446" ], "nt": "TTGCAACACGCAAACACAACCGCACTTTACCTAATTGGCAGTGATGAATCTGGCAAGGGTGATAGCTTTGGCGGAATTGCTGTAAGTGCTGTTTTAATTCATAAGGACAAAATCAATACACTCCACCAAATTGGTGTGGGGGATTCGAAGCAATTTAACGATTACCAAATTAAAGCTTTAGTGCCTAAAATCAAGGCAGCGGTTCATGACCAAGTTACATTAAGCGTTGATGCTAAAACTTACAACCAACTAGTGCAAAGCTTTAAAAATGTCAACGTAATGTTGACCTTTTTGCACTGCAAGGTGTACCACCAGTTGTTGCAACAAAACAAACTAACAGCACAACAATGTGACATTAGCATTGATGAGTTTGCTAATGTCAAACTGTTCACCCAATACACGCAAAAACTCACCAGCCTGCAAAATGAGTTAAAGGAATTAGTTATTCCCAACCACTTCTTAATTAGGGGTGAGAGCTATTCTAAGGTAATTGCCGCTGCTTCGATCTTAGCACGTGCTGCTTTTATTGCACAAATGGAACAGTTAAGCCACCAGTACGGTGTACAGTTTCCCAAGGGTAGTGCCCATGGCATTGTGGAAGCGTTACACTTACTAAAAACAAAAAGGCAGTTTCACAAGTTCGCCCAGTACAGTGCGGTCTGTAAAACTACCTTTAAAAATGTCGCTAGTTTCCTAAAGCAGCTGGCTTAG", "aa": "MQHANTTALYLIGSDESGKGDSFGGIAVSAVLIHKDKINTLHQIGVGDSKQFNDYQIKALVPKIKAAVHDQVTLSVDAKTYNQLVQSFKNVNVMLTFLHCKVYHQLLQQNKLTAQQCDISIDEFANVKLFTQYTQKLTSLQNELKELVIPNHFLIRGESYSKVIAAASILARAAFIAQMEQLSHQYGVQFPKGSAHGIVEALHLLKTKRQFHKFAQYSAVCKTTFKNVASFLKQLA", "aa_hexdigest": "a21daf5ea682856f414fb271068b4c4c", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000139442", "ncbi_nrp_id": "WP_010874475.1", "uniref100_id": "UniRef100_P75446", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139442", "RefSeq:WP_010874475.1", "UniRef:UniRef100_P75446" ] }, "ips": { "uniref100_id": "UniRef100_P75446", "uniref90_id": "UniRef90_P75446" }, "psc": { "uniref90_id": "UniRef90_P75446", "gene": "rnhC", "product": "Ribonuclease HIII", "ec_ids": [ "3.1.26.4" ], "uniref50_id": "UniRef50_P75446", "cog_id": "COG1039", "cog_category": "L", "go_ids": [ "GO:0005737", "GO:0032299", "GO:0046872", "GO:0003723", "GO:0004523", "GO:0043137", "GO:0006298" ] }, "pscc": { "uniref50_id": "UniRef50_P75446", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75446" ], "product": "Ribonuclease HIII" }, "genes": [ "rnhC" ], "id": "APKECPOBEN_307", "locus": "GCA002090215_00270" }, { "type": "cds", "sequence": "contig_1", "start": 152544, "stop": 153269, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00226.36" ], "nt": "ATGGCAGAGGCAAAACGTGATTATTATGAAGTCTTAGGGCTTAGTCGGGATGCTGATGATAACGACATAAAAAAGGCCTTTCGTAAACTAGCCAAAAAGTACCACCCCGACCGCAATAAGGCACCGGATGCGGCTCAAATTTTTGCCGAAATTAACGAGGCCAATGACGTGTTGTCTAACCCCAAAAAACGTGCTAACTATGACAAATACGGCCATGATGGGGTGGACAATGAAGGTGGTTTTGCCTTTCAAGCTGATGTGTTTGATTCTTTTTTTGAGGAGATTGAAAAATCGGGTGCCTTTGATAATTTAAGTGAGTCTAACACCAAAAAGAAAGAGAAGACTAAAACTAAGAAGAAGGGTTGGTTTTGGGGCAAATCCAAACAGGAAGAATCTACCAGCGATACTACTGAATATGCCGATGTTGACGCTGGTTTAGAAGATTATCCGCCTCAATCTGATTATCCAGATGATATTCCTGATGTGGATGCCAGGATCGAAGAAGTGGATCAATCTGCCTATGCCGATGACATTCCCGATGTGGATGCGGGAATGGATTGGGAACAAAACGCAGAAGTAGCAAATTCTGCTAGTGAAATCATCCCCGATGTCGATGCTGGTTTAGCGGATGAGTTTAATACTAGTAGTGCAGCACCCCAAGCTAGTGACTGGGAAGCAATGATTGGTAATCCTGAGTACGGTTACTTTGATGCTGCTGGTGAGTGA", "aa": "MAEAKRDYYEVLGLSRDADDNDIKKAFRKLAKKYHPDRNKAPDAAQIFAEINEANDVLSNPKKRANYDKYGHDGVDNEGGFAFQADVFDSFFEEIEKSGAFDNLSESNTKKKEKTKTKKKGWFWGKSKQEESTSDTTEYADVDAGLEDYPPQSDYPDDIPDVDARIEEVDQSAYADDIPDVDAGMDWEQNAEVANSASEIIPDVDAGLADEFNTSSAAPQASDWEAMIGNPEYGYFDAAGE", "aa_hexdigest": "b22a96e26c130cdba3c187414383c4f4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00226.36", "name": "DnaJ", "length": 63, "aa_cov": 0.2572614107883817, "hmm_cov": 1.0, "evalue": 1.3508622823024666e-27, "score": 91.14558410644531, "start": 7, "stop": 68 } ], "seq_stats": { "molecular_weight": 26822.45639999997, "isoelectric_point": 4.249249076843263 }, "id": "APKECPOBEN_308", "locus": "GCA002090215_00271" }, { "type": "cds", "sequence": "contig_1", "start": 153465, "stop": 154217, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAACCGGAAACTGAACCAGAACCAATTATTACCTTAAGTTCAGAGCCAGTGGAAGCACCAGCTTCTGTTGTGATTGAACCTACCCCTGAAATTGAGGAAACAACTAGTGCTGTAGAAATGGATGCTTCAGTTAAAGCAGATGTTTCTGATGAAGCTGACGCAACTAATGAACCAACTGAACAGGATACCATTTCAGAGCCAGAACAAGAAACAGACGCGGCTGCATTAGAAGAAATTAACCACACCACTGCTGATTTGGAACCAGCAGAAGTTAGTGCTACTAATGATCTAGAACAAGATGTAGTAGAGAAAGTTAATTTTTCAGAACCAGAATCAACAGTAGATACTGCTGCAACCGATCCTGTTGTAGAACAAGCTACGGAAACCTCCACCAATGGTTTCAAATTCTTTAACTTTAGTTCGTTTGTTTTAAGTGACCAAAACCCCAATCCACAAACACCCACCCACCATGAAGAAGATGCTGCAGCCCCCGAGCCAACCGTTGATGAAACTTCTGGAGAATCCACAGCCCCCGAAGTTACCATAGCTGAGTCAACTGTAGAATTGGAAACCGCAGCGGAAATTAATAACCCCGCTACCTTTGTGGAAGAATACCTCCAACCAACTAAAACTACGGTTGTTGATAAATTAGATGAACCAACTGTAGCAAAACCTACTGTTAGCGATTCTGAAAATTCAGTTGCACCAGAACCGGAATTTGTTGCTGGACCGGAACAAACCTTTAGCTGA", "aa": "MQPETEPEPIITLSSEPVEAPASVVIEPTPEIEETTSAVEMDASVKADVSDEADATNEPTEQDTISEPEQETDAAALEEINHTTADLEPAEVSATNDLEQDVVEKVNFSEPESTVDTAATDPVVEQATETSTNGFKFFNFSSFVLSDQNPNPQTPTHHEEDAAAPEPTVDETSGESTAPEVTIAESTVELETAAEINNPATFVEEYLQPTKTTVVDKLDEPTVAKPTVSDSENSVAPEPEFVAGPEQTFS", "aa_hexdigest": "2a5129e0c410e37a1c290f24042dae4a", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 26738.130400000002, "isoelectric_point": 4.0500284194946286 }, "id": "APKECPOBEN_309", "locus": "GCA002090215_00272" }, { "type": "cds", "sequence": "contig_1", "start": 154665, "stop": 155276, "strand": "+", "frame": 3, "gene": "dnaJ", "product": "DnaJ C-terminal domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0000557D12" ], "nt": "TTGAACGAACAGCTCAAGGAAATTAGGTACACCCGTAAGTTAGTCGATCCACAAACTCAGGTAACCACCACTGAATCAATTACCCTAGAAGTTCAGCTATCACATAAATCCCAAACGGAAGCAATTGCCATCTTTAAGGGCTTTGGTCATGACTATGGTAGTGGCTGTGGTGACCTTAAGGTAGTACTTAAAGTGATCCCTAGTGTCTTTTTCCAACTGCAAGCTGATGGCTTACACACCGCTGCCCTAGTCGACCCTCTAGTGGCTTACAACGGTGGTTTAATTGATGTCTTTGGTCCAGTTAACAGCTTTAAAGTTGATATTGAAGGTGGGATTGCTAACAACGATTTAATTGAGTTTAACCAGTTAGGAGTACTAAGAACTAAGACCAAACGTGGTAGTTTATACGTCCACTTGTACTATAGTAGTGTAGCCAAAAAGGGCACTAAGACTAACTGCCAAGTGCAACAGTTCTTTGATCTAGTTCATGTGGAATACAAGCTGTTAAACTACAACTTAAAACAACTCCACAACTACCACTCGGCTCTCACTGCCCAGAAAAAAACCTTGGACCGCAAAAGCTATCAATGTTTAGCAGTGGAAAGCCATTAA", "aa": "MNEQLKEIRYTRKLVDPQTQVTTTESITLEVQLSHKSQTEAIAIFKGFGHDYGSGCGDLKVVLKVIPSVFFQLQADGLHTAALVDPLVAYNGGLIDVFGPVNSFKVDIEGGIANNDLIEFNQLGVLRTKTKRGSLYVHLYYSSVAKKGTKTNCQVQQFFDLVHVEYKLLNYNLKQLHNYHSALTAQKKTLDRKSYQCLAVESH", "aa_hexdigest": "b7d736f707a9ff6d74cfa66270896323", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0000557D12", "query_cov": 0.9014778325123153, "subject_cov": 0.9838709677419355, "identity": 0.541, "score": 195.0, "evalue": 3.35e-60, "valid": false, "product": "DnaJ C-terminal domain-containing protein", "uniref50_id": "UniRef50_UPI0000557D12" }, "pscc": { "uniref50_id": "UniRef50_UPI0000557D12", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0000557D12" ], "product": "DnaJ C-terminal domain-containing protein" }, "genes": [ "dnaJ" ], "id": "APKECPOBEN_310", "locus": "GCA002090215_00273" }, { "type": "cds", "sequence": "contig_1", "start": 155299, "stop": 155751, "strand": "+", "frame": 1, "gene": "grpE", "product": "Protein GrpE", "db_xrefs": [ "COG:COG0576", "COG:O", "GO:0000774", "GO:0005737", "GO:0006457", "GO:0042803", "GO:0051082", "GO:0051087", "KEGG:K03687", "SO:0001217", "UniRef:UniRef50_P47443", "UniRef:UniRef90_P78017" ], "nt": "ATGAGCGAAAACTCACTCACAATTACCGAAATTCTCAGTTCCATCCGTACGCTTTTAGTCAAACACAATAAGGCTAAAGTAACCCAAATTGAAAAGGAACTTTTACAAGCAGTTGCTGAACTGGAAAAGAAGTTTAAGCAACAAGTACAAAACTTCAACGAGTTGCAACAAAAGATTCCTAACTTACAAAAAGTCAACGAGGAATTTCGCTTAAAAGTGGAAAAAATCCAGGAAGAAGCGCAGAAGAAAATTCAGGAAAAAGTGGCAGAACTGACCATTAAAAGTAAGGAAGAGTTGGAAAATGCAAAGAAGTATGTGATCGAAAAGTCGATTGATCAACCCTTAATTATTATTGACCAGTTCGAAATTGCACTTTCCTATGCTCAAAAGGATCCCCAAGTAAAAAACTATACCACTGGGTTTAACATGGTGTTGGATGCCTTTTCGCGCTGA", "aa": "MSENSLTITEILSSIRTLLVKHNKAKVTQIEKELLQAVAELEKKFKQQVQNFNELQQKIPNLQKVNEEFRLKVEKIQEEAQKKIQEKVAELTIKSKEELENAKKYVIEKSIDQPLIIIDQFEIALSYAQKDPQVKNYTTGFNMVLDAFSR", "aa_hexdigest": "b4cb6dcd106da9db7a74149b5bbc8cc3", "start_type": "ATG", "rbs_motif": null, "genes": [ "grpE" ], "truncated": "3-prime", "pseudogene": { "start": 155299, "stop": 155952, "observations": { "mutation": [ 155749 ], "stop": [ 155749 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P78017", "query_cov": 1.0, "subject_cov": 0.6912442396313364, "identity": 1.0, "score": 266.0, "evalue": 6.31e-89, "gene_start": 1, "gene_end": 150, "reference_start": 1, "reference_end": 150, "reference_sequence": "MSENSLTITEILSSIRTLLVKHNKAKVTQIEKELLQAVAELEKKFKQQVQNFNELQQKIPNLQKVNEEFRLKVEKIQEEAQKKIQEKVAELTIKSKEELENAKKYVIEKSIDQPLIIIDQFEIALSYAQKDPQVKNYTTGFNMVLDAFSRWLEGFGVTKIAIEPGAQFDEKVMAALEVVPSDQPANTVVKVSKSGYKLHDKVIRFASVVVSQGNKTE" }, "paralog": false, "identity": 0.9953917050691244, "score": 409.0, "evalue": 1.86e-147, "description": "Internal stop codon at 155749. Nonsense mutation around 155749.", "psc": { "uniref90_id": "UniRef90_P78017", "gene": "grpE", "product": "Protein GrpE", "uniref50_id": "UniRef50_P47443", "cog_id": "COG0576", "cog_category": "O", "kegg_orthology_id": "K03687", "go_ids": [ "GO:0005737", "GO:0000774", "GO:0042803", "GO:0051087", "GO:0051082", "GO:0006457" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P78017", "UniRef:UniRef50_P47443", "COG:COG0576", "COG:O", "KEGG:K03687", "GO:0005737", "GO:0000774", "GO:0042803", "GO:0051087", "GO:0051082", "GO:0006457" ] }, "pscc": { "uniref50_id": "UniRef50_P47443", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47443" ], "product": "Protein GrpE" } }, "id": "APKECPOBEN_311", "locus": "GCA002090215_00274" }, { "type": "cds", "sequence": "contig_1", "start": 155815, "stop": 155952, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAGCACTGGAAGTTGTGCCTTCTGATCAACCGGCTAATACGGTGGTGAAGGTATCGAAATCGGGTTACAAACTCCACGACAAGGTCATTCGCTTTGCTTCCGTAGTGGTAAGCCAAGGTAATAAAACAGAATAA", "aa": "MAALEVVPSDQPANTVVKVSKSGYKLHDKVIRFASVVVSQGNKTE", "aa_hexdigest": "55c9b99d6ac59d5e21650b09cf378f68", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4828.501900000002, "isoelectric_point": 9.302868461608885 }, "id": "APKECPOBEN_312", "locus": "GCA002090215_00275" }, { "type": "cds", "sequence": "contig_1", "start": 155952, "stop": 156317, "strand": "+", "frame": 3, "gene": null, "product": "MPN121 family protein", "db_xrefs": [ "RefSeq:WP_010874478.1", "SO:0001217", "UniParc:UPI0000139461", "UniRef:UniRef100_P75353", "UniRef:UniRef50_P75353", "UniRef:UniRef90_P75353" ], "nt": "ATGAGCACAAATAAAAGAAGAACCATTCAGATTGAAATCACTGAAGAACACTTCAAGGATTTAGAAAAAGCCTTGGAAGCCTTGAAGGGTACCCAATTACCCTTCTCCACTACCGTAGAACAGTTTGTGGAATTAATTTTGTCCAACTATGTCGCTACTTCCAACAAGATTTCTAATTTAGCTGAAAGTGGCTTTGATGTGGCTTCTATTCAACAAGAATTAGAAAAAATTGGCAGCGCTGCAGGGGCGGATGATGCGCTCAAAAGCTTCTTGGATGAGTTGTTAAAAACATCGCAAAAAAGCTTTAGCAATACCAAAGATGGCAAAAAAAATGATGACGATAATAATTCGTCATCAAAATCATAA", "aa": "MSTNKRRTIQIEITEEHFKDLEKALEALKGTQLPFSTTVEQFVELILSNYVATSNKISNLAESGFDVASIQQELEKIGSAAGADDALKSFLDELLKTSQKSFSNTKDGKKNDDDNNSSSKS", "aa_hexdigest": "58ad45def04906f3e98d12365a0a2c20", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000139461", "ncbi_nrp_id": "WP_010874478.1", "uniref100_id": "UniRef100_P75353", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139461", "RefSeq:WP_010874478.1", "UniRef:UniRef100_P75353" ] }, "ips": { "uniref100_id": "UniRef100_P75353", "uniref90_id": "UniRef90_P75353" }, "psc": { "uniref90_id": "UniRef90_P75353", "product": "MPN121 family protein", "uniref50_id": "UniRef50_P75353" }, "pscc": { "uniref50_id": "UniRef50_P75353", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75353" ], "product": "Uncharacterized protein MG202 homolog" }, "genes": [], "id": "APKECPOBEN_313", "locus": "GCA002090215_00276" }, { "type": "tRNA", "sequence": "contig_1", "start": 156335, "stop": 156408, "strand": "-", "gene": "trnT", "product": "tRNA-Thr(ggt)", "amino_acid": "Thr", "anti_codon": "ggt", "score": 62.8, "nt": "GCCGAATTAGTTTAGGGGCAAAACAGATCCATGGTAAGGATCAGAGAACAGTTCGACTCTGTTATTCGGCACCA", "db_xrefs": [ "SO:0000270" ], "anti_codon_pos": [ 156374, 156376 ], "id": "APKECPOBEN_34", "locus": "GCA002090215_00277" }, { "type": "cds", "sequence": "contig_1", "start": 156443, "stop": 158098, "strand": "+", "frame": 2, "gene": "parE", "product": "DNA topoisomerase IV subunit B", "db_xrefs": [ "COG:COG0187", "COG:L", "EC:5.6.2.2", "GO:0003677", "GO:0003918", "GO:0005524", "GO:0005694", "GO:0006265", "GO:0034335", "GO:0046872", "SO:0001217", "UniRef:UniRef50_P47445", "UniRef:UniRef90_P78016" ], "nt": "ATGAAAAATAATTACAGCGAAGCGAATATCAAAATTCTCAAGGGGCTAGATGCTGTAAAAAAACGCCCGGGAATGTACATCGGCTCAACCGATAGTCGCGGTTTTCACCACTTGCTGTGGGAAATTTTAGACAACTGTGTCGATGAAGTTTTATCGGGCTTTGCTAATACTATTGCCGTTGTTTTACATGCCGAAAACCAGATAACTGTTAGTGACAACGGTCGTGGTATTCCCTTCGAAACACACTCCGATTCCAAGATTTCTACGATTGACACCGTCTTTACCTACCTCCATGCCGGTGGTAAGTTTGATAACGATTCTTATAAGATTGCTGGAGGGTTGCACGGGGTGGGGGCTTCGGTGGTGAATGCCTTGAGTGACCAGTTACAAGTAACGGTTAAACGTCAGGGCAAGGTGTACCGCTCGGTGTATGAAAATGGTGGTAAGATTAAGCAAAAAGCGCATTGCATTGGTAATGCCAAAATTGACGAACACGGTACCAGTGTGACTTTTCGTCCTGACCCAAAGGTCTTTAAAAAGATCCACTTTGACAGCGAACTAATTCGTGCGCGGTTAAAGGAATTAGCCTTCCTTTTCAAAAAGTTACAACTGACCTTCGTTGATGAAACCGGCAGTGGGGAAAAAGAGGTGTTCTTTACCGAAGCCGGTATTAGTCAGTACTTGGACGAACTTAACGCTGATTCCAAACAAATTGCGCAAAAGATCTTTGTTAGTGGCACTGAAGATGACATTGAACTGGAAGCCGTGTTTCAGTTTATTGATGGGGAGGATGAAAAACTACTTTCGTTTGCCAATTCGATTCGCACCAGCGAAGGGGGTAGTCATGAAGCGAGTTTTCGTCAATCTGTTGGTGATGTGATTAACAACTACTGCCGCAAGTACAACTTCCTAAAGGAGCGTGACAAGAACTTTGAGGCCAGTGAAATCCGTGAAGGCTTAAACGGCATTATTAAGGTCAATCTCCCCGAAAAGATTATTGCCTTTGAAGGGCAAACGAAGAGCAAACTGTTCTCCAAGGAAGTTAAAGCGGTAGTGCAAAAGCTAACCCAAAAGCACTTCTTCCAGTTCTTGGAACGTAACAGTGTTGATGCTAAATTAATTGTCGAAAAGCTGTTTTATAACCGCAAACTGCGCCAAGAACTCAAACAACAACGCCAAATTAAAAAGAACCTTTCTAATCCGAAGGCCGAGCGCATTTTGTTTGGTAAATTAGCACCGGCGCAGTCCAAAAAAGTGGCTGAACGCGAACTGTTTGTGGTGGAAGGTGACAGTGCTGGTGGTACGGCTAAAATGGGACGAGATCGCTTTCTACAAGCCATCTTACCGTTACGCGGTAAGGTGTTAAATGTGGAGAAAATTAACAACAAGAAGGAAGCGATTAACAACGAAGAGCTGTTAACACTCATCTTCTGTATCGGTACTGGTATTGGTAACAACTTCACCATTCGCGATCGTAAATACGACAAGATTATTATCATGACTGATGCTGACAATGACGGTGCGCACATCCAGATTTTACTGCTCACCTTCTTCTACCGTTACATGAAACCCCTAATTGAAAAAGGACACATTTACCTAGCACTTCCACCACTTTATAAGTTTGAAGGTCGTGATAAAAAAGCCCGTTATCTCTGA", "aa": "MKNNYSEANIKILKGLDAVKKRPGMYIGSTDSRGFHHLLWEILDNCVDEVLSGFANTIAVVLHAENQITVSDNGRGIPFETHSDSKISTIDTVFTYLHAGGKFDNDSYKIAGGLHGVGASVVNALSDQLQVTVKRQGKVYRSVYENGGKIKQKAHCIGNAKIDEHGTSVTFRPDPKVFKKIHFDSELIRARLKELAFLFKKLQLTFVDETGSGEKEVFFTEAGISQYLDELNADSKQIAQKIFVSGTEDDIELEAVFQFIDGEDEKLLSFANSIRTSEGGSHEASFRQSVGDVINNYCRKYNFLKERDKNFEASEIREGLNGIIKVNLPEKIIAFEGQTKSKLFSKEVKAVVQKLTQKHFFQFLERNSVDAKLIVEKLFYNRKLRQELKQQRQIKKNLSNPKAERILFGKLAPAQSKKVAERELFVVEGDSAGGTAKMGRDRFLQAILPLRGKVLNVEKINNKKEAINNEELLTLIFCIGTGIGNNFTIRDRKYDKIIIMTDADNDGAHIQILLLTFFYRYMKPLIEKGHIYLALPPLYKFEGRDKKARYL", "aa_hexdigest": "e6a3b0353c31848adc3c658227051626", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P78016", "query_cov": 1.0, "subject_cov": 0.8677165354330708, "identity": 1.0, "score": 1075.0, "evalue": 0.0, "valid": true, "gene": "parE", "product": "DNA topoisomerase IV subunit B", "ec_ids": [ "5.6.2.2" ], "uniref50_id": "UniRef50_P47445", "cog_id": "COG0187", "cog_category": "L", "go_ids": [ "GO:0003677", "GO:0003918", "GO:0005524", "GO:0005694", "GO:0006265", "GO:0034335", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_P47445", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47445" ], "product": "DNA topoisomerase 4 subunit B" }, "genes": [ "parE" ], "id": "APKECPOBEN_314", "locus": "GCA002090215_00278" }, { "type": "cds", "sequence": "contig_1", "start": 158111, "stop": 158350, "strand": "+", "frame": 2, "gene": null, "product": "DNA topoisomerase (ATP-hydrolyzing)", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_T0TWZ4" ], "nt": "TTGGAACAGTACCGTGCTAAGCATAGCCACTTTAATGTGCAACGGTACAAGGGTTTAGGGGAAATGAATGCTGATCAGCTCTGGGAAACCACGATGAACCCGATGACGCGTAAACTAATTCAGGTCAAATTGGATAACTTTATCCAAGCTGAAAAGCAAATTAATGTTTTTATGGGTGATAAAACCGAATTGCGTAAAAGCTGGATTGAAGCTAACATTAACTTTAGTAGTGAGAACTAA", "aa": "MEQYRAKHSHFNVQRYKGLGEMNADQLWETTMNPMTRKLIQVKLDNFIQAEKQINVFMGDKTELRKSWIEANINFSSEN", "aa_hexdigest": "5b4366a3a2c34657f08d4ad6e69b2d63", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A1Q6V0H2", "query_cov": 0.8607594936708861, "subject_cov": 0.8395061728395061, "identity": 0.618, "score": 91.3, "evalue": 1.35e-22, "valid": false, "product": "DNA topoisomerase (ATP-hydrolyzing)", "uniref50_id": "UniRef50_T0TWZ4" }, "pscc": { "uniref50_id": "UniRef50_T0TWZ4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_T0TWZ4" ], "product": "DNA topoisomerase (ATP-hydrolyzing)" }, "genes": [], "id": "APKECPOBEN_315", "locus": "GCA002090215_00279" }, { "type": "cds", "sequence": "contig_1", "start": 158350, "stop": 158637, "strand": "+", "frame": 1, "gene": null, "product": "DNA gyrase subunit A", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9XCU2" ], "nt": "ATGGAAAAGAATAAACAAGCGTTATTACTCCAGGCCATCGAAGATGTCTTTGCCTTTAGCTTTAGTAAGTATGCTAAGTACATTATCCAAGACCGGGCTTTACCTGATCTACGTGATGGGCTTAAACCAGTTCAACGCCGCATCCTCTATGGCATGTACCAAATGGGCTTAAAACCCACTTCACCCTACAAAAAGTCAGCTCGGGCAGTCGGAGAAATTATGGGTAAGTACCACCCCCATGGCGATGCATCGATCTATGATGCGATTGTAAGGATGTCACAAGCCTGA", "aa": "MEKNKQALLLQAIEDVFAFSFSKYAKYIIQDRALPDLRDGLKPVQRRILYGMYQMGLKPTSPYKKSARAVGEIMGKYHPHGDASIYDAIVRMSQA", "aa_hexdigest": "5a748c3ede98040626375442c755abc5", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0028540D82", "query_cov": 0.8736842105263158, "subject_cov": 0.83, "identity": 0.6629999999999999, "score": 124.0, "evalue": 3.5e-35, "valid": false, "product": "DNA gyrase subunit A", "uniref50_id": "UniRef50_Q9XCU2" }, "pscc": { "uniref50_id": "UniRef50_Q9XCU2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9XCU2" ], "product": "DNA gyrase subunit A" }, "genes": [], "id": "APKECPOBEN_316", "locus": "GCA002090215_00280" }, { "type": "cds", "sequence": "contig_1", "start": 158707, "stop": 160719, "strand": "+", "frame": 1, "gene": "parC", "product": "DNA topoisomerase IV subunit A", "db_xrefs": [ "COG:COG0188", "COG:L", "EC:5.6.2.2", "GO:0003677", "GO:0003918", "GO:0005524", "GO:0005694", "GO:0005737", "GO:0005886", "GO:0006265", "GO:0009330", "GO:0034335", "SO:0001217", "UniRef:UniRef50_P47446", "UniRef:UniRef90_P75352" ], "nt": "ATGCGCTATACCGAAGCGCGTTTAAGTCCGTACGGGTTTGAACTACTCAAAGACATTGAAAAGCAGTTAGTACCGTTTGTGAATAACTTTGATGACAGTGAGGTCGAACCGAGCGTGTTACCAACCCTGTTACCTAACCTCTTTATTAACGGTACCAGTGGAATAGCGGCGGGTTATGCCACTAATATTGCACCCCATAATGTCGGTGAACTGCTTGATGGTTTGAGTTACCGGATTGAAAACCCTGATTGTGATTTAAAGGCCATCTTAAAGATTGTTAAGGGACCGGATTTTCCAACTGGGGGATTAGTGTACTTTGAGCAAGAGTTGGCGAACATCTACCAAACTGGGAAGGGTAAGTTTGTGATTCAGGCCAAATATGAAACGAACACTGCGTTTGGCCAAAACCAGATTGTAATCACCGAAATCCCATACGAAACGGTGAAGGCCAATATTGTTAAACAAATTGAAGAGTTAATTAGTGACAATAAACTCAGTGCCTTGGAAAGTGTGATTGACAGCTCTGATCGTAGTGGTATTCGAATCATTATTAACCACAAGGACTTTTTGAGTGCTGACAAGATTATGGCGTTCTTGTTCAAACACACCCAACTCCAAGTAAACTTTAACCTCAACAATACGGTAATTGCCAACCGTTGTCCGGTACGGGTGGGGTTGTTAGCTTACTTTGATCAGTTCCTAGCCTTTGCCCACGAATTGATCATTAACAGCGCCAAGTATGATTTAGCATTGGCCAATAAGCGCTTGGAAATCATTAAGGGCTTAATTAAGGCGGTTTCCATGATTGATGAAATTATTCGTTTAATTCGCCGTGCTACTGACAAACAGGATGCGAAAACCAAGCTCATTGACAAGTATGCTTTTACCCTAAATCAAGCCGAAGCGATTGTGTCGTTGCGTTTGTACCAGTTAACTAACACCGACATTAAGGTCCTGTTTGCCGAACAAAAAGAGTTAGAGCAAACGATCCAAACAGCCGAACGCTTAATTGCTAAACCGCAAGCACGAAACCAGTTGTTACAAGCACAGTTTTTCCAATACAAAAAGCAGTTTAACCAACCACGACGCGCACAAATAGTTGGTTTAATTGAAAAGCAAAAGGTACAAGACAGTGACTTCATCGAACACAAGGAAGTGGGCTTACTCATTAGTCACGATGGCATCTACTTTAAGTTTGAACCAGAACAGTTAGCGAAGCACCTAGTGGAATTTAAAAGTGAACAAGACCAACTGATTTTTGGAGGCGTAGTACAAAACTCCGATTACTTTTTTATGGTGACTTCACTCGGTAACATTATTACCGTTCCCATTTACAAAACGTTAAGTAACACCAAAACCAAGATGAACGAGTTGTTGGCCAAAAAACCGATCCTAATGGAAGATGAAAAATTGGTACTAGCAGGGATTGTAAACCCAGACAAAATGGAACAACAGCTCTTGGTGTTAACGTCACAGTGCGGTATGGTCAAACGGGTCGAGTTGAGCAAAGTCATTAACACGAAGCAAATTAAGTCAAGCTGCTGCATGGCACTCCGGGAACGCGACAAACTGGTCAACGCCTTTGTTCAAACTAAAGGGGAACCTAAACTAGTTTGTCTGGTGTCATCTTCCAACAGTTTTGCTACCTTCTTAGCGGAAGAAATTCCAATTATTAGTAACAAGGGAATTGGGGTGAAGGGGATTAAACTCAAAGCTGAGGAAAAGGTACGGTTTGCAATGCCGTTACAGGATAATGATGCCTTAGTAGTGATTAACAGTGATGGTGGTGTCTATAACTTTGAAGTAGTGGAACTGGCAGTCGCTAGCCGCATGAGTGTTGGTAAAAAACTAATTCCCAAAACCAAAACCCCGGTAAGCTGTTTTGCTGCTAATAAGCACAGTGAGATTATTGGTCATCGTGGTAAAAATGGTAGTGATCTGTTTACCCTAAATGAGTTAAACCGTTTACCAAAGTCAACCGTTAGTCAAATGCGTTTGTTTAAGTGGAGCTAG", "aa": "MRYTEARLSPYGFELLKDIEKQLVPFVNNFDDSEVEPSVLPTLLPNLFINGTSGIAAGYATNIAPHNVGELLDGLSYRIENPDCDLKAILKIVKGPDFPTGGLVYFEQELANIYQTGKGKFVIQAKYETNTAFGQNQIVITEIPYETVKANIVKQIEELISDNKLSALESVIDSSDRSGIRIIINHKDFLSADKIMAFLFKHTQLQVNFNLNNTVIANRCPVRVGLLAYFDQFLAFAHELIINSAKYDLALANKRLEIIKGLIKAVSMIDEIIRLIRRATDKQDAKTKLIDKYAFTLNQAEAIVSLRLYQLTNTDIKVLFAEQKELEQTIQTAERLIAKPQARNQLLQAQFFQYKKQFNQPRRAQIVGLIEKQKVQDSDFIEHKEVGLLISHDGIYFKFEPEQLAKHLVEFKSEQDQLIFGGVVQNSDYFFMVTSLGNIITVPIYKTLSNTKTKMNELLAKKPILMEDEKLVLAGIVNPDKMEQQLLVLTSQCGMVKRVELSKVINTKQIKSSCCMALRERDKLVNAFVQTKGEPKLVCLVSSSNSFATFLAEEIPIISNKGIGVKGIKLKAEEKVRFAMPLQDNDALVVINSDGGVYNFEVVELAVASRMSVGKKLIPKTKTPVSCFAANKHSEIIGHRGKNGSDLFTLNELNRLPKSTVSQMRLFKWS", "aa_hexdigest": "8d329cdec9f77bdaf3996001417a5777", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75352", "query_cov": 1.0, "subject_cov": 0.8491761723700887, "identity": 1.0, "score": 1288.0, "evalue": 0.0, "valid": true, "gene": "parC", "product": "DNA topoisomerase IV subunit A", "ec_ids": [ "5.6.2.2" ], "uniref50_id": "UniRef50_P47446", "cog_id": "COG0188", "cog_category": "L", "go_ids": [ "GO:0003677", "GO:0003918", "GO:0005524", "GO:0005694", "GO:0005737", "GO:0005886", "GO:0006265", "GO:0009330", "GO:0034335" ] }, "pscc": { "uniref50_id": "UniRef50_P47446", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47446" ], "product": "DNA topoisomerase 4 subunit A" }, "genes": [ "parC" ], "id": "APKECPOBEN_317", "locus": "GCA002090215_00281" }, { "type": "cds", "sequence": "contig_1", "start": 160706, "stop": 160957, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCCTTTATGAACCAAAACAACCAGACTACTAATATTACGTTTGGTGACAAGTTAGGATTAGAAGGGGAAGAGGTTTCGGTAGCTTCTACTCTTATTAATACCACCAACGAAAGTAAGCACCAACTAGCGATAGTGGGTCCCACCCGGATGGACTACCAAAAGGTGAAAGCGTTATTGTTGACCCTCAAAGAAGAGATCGAGGAGTACGATAAGCAACTTCACGGTGGTAAAACCACTAGCTCCACTTAA", "aa": "MAFMNQNNQTTNITFGDKLGLEGEEVSVASTLINTTNESKHQLAIVGPTRMDYQKVKALLLTLKEEIEEYDKQLHGGKTTSST", "aa_hexdigest": "6158fecdc70c3a4303ea66eef2e7d5af", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9202.240299999998, "isoelectric_point": 5.237963294982909 }, "id": "APKECPOBEN_318", "locus": "GCA002090215_00282" }, { "type": "cds", "sequence": "contig_1", "start": 160964, "stop": 161761, "strand": "-", "frame": 3, "gene": "hrcA", "product": "heat-inducible transcriptional repressor HrcA", "db_xrefs": [ "COG:COG1420", "COG:K", "GO:0003677", "GO:0006355", "GO:0045892", "KEGG:K03705", "SO:0001217", "UniRef:UniRef50_P75351", "UniRef:UniRef90_P75351" ], "nt": "ATGAAAAATTTAACTACCCGACAAGCCCAAATTCTCAAGGCCATCATCAACGAATATATTGCCTATCCAGTACCTGTTGGTAGTAAATTATTAACTAAAAAATATTTTAAAAACCTCTCTGGCGGTACACTCCGAAACGAAATGGCCGTTTTGGAAAAAGAGGGTTATCTCAAAAAGAACCACATCTCTTCAGGACGAATCCCCTCCCAGTTGGGCTATCAGTACTATGTCAAGTTACTCACTAAAAATGATGATAAGAGTAACCTTAAAACCCGCTTAAGGGCGATTATCTTGCAAAAACACAAAACAATTGATGAAATTATTGAACTGGGGGTAAAGTTTATCAACGAAATGGTAAACCTACCAGTGGTGTTAACCCACTTTAGTAGTGATGAAGTCTTAAAAAAGATTGATCTCATTATGCTCGATCAGTCCTGTGCACTGCTACTGTTAGTGTCAGCTAGTGGTAACGTCTTTAAGAAAACGATTAGCTATGCTAACCAAAGACAGTTTGAAGACATCATGGTATGTGTCAGGATCTTTAATGATCGCATTATCGATACACGTTTCTGTGATATTGCCCAACACTTAGATGTTTTAAAGGAAATTATTCGCAGCAAGGTACACGAGTACCAGTATGTCATTGACGAAATCCTGTTTAAGCTGTTTAACTTTGAGGAATTCCAACAAGCACGTAAGCAGGTTTATGGCATCCATTACCTAGCCCAACAACCAGAGTTTGCCAACCAGGAAAGATTGACGCGTATCTTAAACTTACTGGAAGATACTAGTGTCTGA", "aa": "MKNLTTRQAQILKAIINEYIAYPVPVGSKLLTKKYFKNLSGGTLRNEMAVLEKEGYLKKNHISSGRIPSQLGYQYYVKLLTKNDDKSNLKTRLRAIILQKHKTIDEIIELGVKFINEMVNLPVVLTHFSSDEVLKKIDLIMLDQSCALLLLVSASGNVFKKTISYANQRQFEDIMVCVRIFNDRIIDTRFCDIAQHLDVLKEIIRSKVHEYQYVIDEILFKLFNFEEFQQARKQVYGIHYLAQQPEFANQERLTRILNLLEDTSV", "aa_hexdigest": "53d5a70a4a46a8ca087e08370db515f7", "start_type": "ATG", "rbs_motif": "AATAA", "genes": [ "hrcA" ], "truncated": "5-prime", "pseudogene": { "start": 160706, "stop": 161761, "observations": { "mutation": [ 160966 ], "stop": [ 160966 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75351", "query_cov": 1.0, "subject_cov": 0.7549857549857549, "identity": 1.0, "score": 510.0, "evalue": 2.98e-181, "gene_start": 1, "gene_end": 265, "reference_start": 1, "reference_end": 265, "reference_sequence": "MKNLTTRQAQILKAIINEYIAYPVPVGSKLLTKKYFKNLSGGTLRNEMAVLEKEGYLKKNHISSGRIPSQLGYQYYVKLLTKNDDKSNLKTRLRAIILQKHKTIDEIIELGVKFINEMVNLPVVLTHFSSDEVLKKIDLIMLDQSCALLLLVSASGNVFKKTISYANQRQFEDIMVCVRIFNDRIIDTRFCDIAQHLDVLKEIIRSKVHEYQYVIDEILFKLFNFEEFQQARKQVYGIHYLAQQPEFANQERLTRILNLLEDTSVWQQMAFMNQNNQTTNITFGDKLGLEGEEVSVASTLINTTNESKHQLAIVGPTRMDYQKVKALLLTLKEEIEEYDKQLHGGKTTSST" }, "paralog": false, "identity": 0.9971509971509972, "score": 682.0, "evalue": 3.54e-251, "description": "Internal stop codon at 160966. Nonsense mutation around 160966.", "psc": { "uniref90_id": "UniRef90_P75351", "gene": "hrcA", "product": "heat-inducible transcriptional repressor HrcA", "uniref50_id": "UniRef50_P75351", "cog_id": "COG1420", "cog_category": "K", "kegg_orthology_id": "K03705", "go_ids": [ "GO:0003677", "GO:0006355", "GO:0045892" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75351", "UniRef:UniRef50_P75351", "COG:COG1420", "COG:K", "KEGG:K03705", "GO:0003677", "GO:0006355", "GO:0045892" ] }, "pscc": { "uniref50_id": "UniRef50_P75351", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75351" ], "product": "Heat-inducible transcription repressor HrcA" } }, "id": "APKECPOBEN_319", "locus": "GCA002090215_00283" }, { "type": "cds", "sequence": "contig_1", "start": 161886, "stop": 162875, "strand": "+", "frame": 3, "gene": "uvrC", "product": "excinuclease ABC subunit UvrC", "db_xrefs": [ "GO:0003677", "GO:0005737", "GO:0006289", "GO:0009380", "GO:0009381", "GO:0009432", "KEGG:K03703", "SO:0001217", "UniRef:UniRef50_P47448", "UniRef:UniRef90_P75350" ], "nt": "TTGTATGTCGGGAAGGCTAAGGACATCTTTAAGCGGGTACACCATTACTTTAATCCAAACCGTTCCTTTAAAACACGGGCACTAGTGGAACGAATTGCGGATGTCGAATACGTCATTTTGAAAAATGAAAATGATGCTTTGAACTTAGAGGCCAAACTAATTAAACAGTACAAACCGCGCTTTAATTTAGTTTTAAAGGAAAATAATGGTTATCTCTATTTCTTTATTACCGCCAGTGTTAAACCCACCTTGGAATTGGGCCGCCGGTATGAGTTTTCCAAGAATAAGTACTTCGGTCCGTTTGCATCGAGTAAGTTTCGACTGCGTGACATTTATGACCTGTTGTTAAAGCTGTTTCCATTACGCAAGTGCGCGCCCCATGAACGGGGTCACCCCTGCTTTTACTATCAGCTTAAAATGTGCATGGGTCAGTGTATGGGCGAGGATACACCGGAACGTTACCAGACCACCGTCAAGGGAATTGAACAGTTTTTTAACCACGGTCCCGAGCAGGTTTTAAACCACTTGCAACAGCAAGAAATCAAGGCTAGTGAACAACAAAACTTTGAAGCAGCACGCCACTTCTTGGACTTACAGAAAGCCGTTTTAGAACTAGTGAACATGCAACAAACGGCCTTCATTAAAGCAAAGCAAAGCCATGACTTTATTGGTTATGTCTTTGAGAAAAATGTGTTAGCCATTACCGTCTTTGCTTACGTTGATAACCAACTAATAGGCAAAAACCAGCAGGTCTTTGAACTACCACAGGACGATGAAAAGGAAGTTGAAAGTGCTTTGGTTACCTTTATTTACCACTATTATTCCACCAATAAGATACCCAAAACCCTTACAGTTTCTTTAAGCGAAGAGAATTTGAGCTTACTGGCCAACAGTCTTAAAATTAATGTTACGCAACCAAAAAACGGCGAGCAAAAATCCATTTTGCAAACCGTGATTGACAACGCCCGTTATGCCTTAAACACCAAGTGA", "aa": "MYVGKAKDIFKRVHHYFNPNRSFKTRALVERIADVEYVILKNENDALNLEAKLIKQYKPRFNLVLKENNGYLYFFITASVKPTLELGRRYEFSKNKYFGPFASSKFRLRDIYDLLLKLFPLRKCAPHERGHPCFYYQLKMCMGQCMGEDTPERYQTTVKGIEQFFNHGPEQVLNHLQQQEIKASEQQNFEAARHFLDLQKAVLELVNMQQTAFIKAKQSHDFIGYVFEKNVLAITVFAYVDNQLIGKNQQVFELPQDDEKEVESALVTFIYHYYSTNKIPKTLTVSLSEENLSLLANSLKINVTQPKNGEQKSILQTVIDNARYALNTK", "aa_hexdigest": "0bb300c07fadff740364e38d732f90b3", "start_type": "TTG", "rbs_motif": null, "genes": [ "uvrC" ], "truncated": "both", "pseudogene": { "start": 161793, "stop": 163178, "observations": { "mutation": [ 161862, 162873 ], "stop": [ 161862, 162873 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75350", "query_cov": 1.0, "subject_cov": 0.5483333333333333, "identity": 0.997, "score": 619.0, "evalue": 4.72e-219, "gene_start": 1, "gene_end": 329, "reference_start": 32, "reference_end": 360, "reference_sequence": "MIIVNNTLAFKLKNAPHKPGCYLWKDDAGQVLYVGKAKDIFKRVHHYFNPNRSFKTRALVERIADVEYVILKNENDALNLEAKLIKQYKPRFNLVLKENNGYLYFFITASVKPTLELGRRYEFSKNKYFGPFASSKFRLRDIYDLLLKLFPLRKCAPHERGHPCFYYQLKMCMGQCMGEDTPERYQTTVKGIEQFFNHGPEQVLNHLQQQEIKASEQQNFEAARHFLDLQKAVLELVNMQQTAFIKAKQSHDFIGYVFEKNVLAITVFAYVDNQLIGKNQQVFELPQDDEKEVESALVTFIYHYYSTNKIPKTLTVSLSEENLSLLANSLKINVTQPKNGEQKSILQTVIDNARYALNTKWTGFINNLNRAEVHQQLAQLLQVPSIQSLEIIDISFYDKDHVVGAMLRYENGKRMKALSRRYNINIDHKGDTNYMADVVYRRIISSIQTHKQLPLSDLLIVDGGIAQINTVTKVFASFPNVTQPIIIGLAKNTRHQTDHIVLTDNTTINIDKNTPLFAYLTTIQEEVDSFAKHNAFKRVSRARFQNPLLQIEGVGRKTVQILLDNFQTMQNIEAASLSELSQFIPANLAQKIKTFLKEND" }, "paralog": false, "identity": 0.9934924078091106, "score": 871.0, "evalue": 6.05e-321, "description": "Internal stop codon at 161862, 162873. Nonsense mutation around 161862, 162873.", "psc": { "uniref90_id": "UniRef90_P75350", "gene": "uvrC", "product": "excinuclease ABC subunit UvrC", "uniref50_id": "UniRef50_P47448", "kegg_orthology_id": "K03703", "go_ids": [ "GO:0003677", "GO:0005737", "GO:0006289", "GO:0009380", "GO:0009381", "GO:0009432" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75350", "UniRef:UniRef50_P47448", "KEGG:K03703", "GO:0003677", "GO:0005737", "GO:0006289", "GO:0009380", "GO:0009381", "GO:0009432" ] }, "pscc": { "uniref50_id": "UniRef50_P47448", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47448" ], "product": "UvrABC system protein C" } }, "id": "APKECPOBEN_320", "locus": "GCA002090215_00284" }, { "type": "cds", "sequence": "contig_1", "start": 162909, "stop": 163595, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF14520.12", "PFAM:PF08459.17" ], "nt": "GTGCACCAGCAATTAGCGCAGCTTTTACAAGTTCCTTCGATTCAAAGTCTGGAAATTATTGACATCTCCTTTTATGACAAAGACCACGTGGTGGGGGCGATGTTACGGTACGAAAATGGCAAACGGATGAAAGCGTTATCACGCCGTTACAACATTAACATTGACCATAAGGGTGACACTAACTATATGGCTGATGTGGTGTACCGCCGGATTATTTCGTCCATCCAAACCCATAAACAGCTACCTTTAAGTGATCTTTTAATTGTCGATGGTGGGATTGCCCAAATTAATACGGTAACCAAGGTGTTTGCCTCTTTCCCTAACGTTACCCAACCGATCATTATTGGCTTAGCGAAAAACACCCGTCACCAAACTGACCACATTGTGTTAACGGATAACACTACCATTAACATTGACAAAAATACGCCTTTGTTTGCTTATCTTACCACCATTCAAGAAGAGGTCGATAGCTTTGCCAAACACAATGCCTTTAAGCGAGTTAGTCGCGCTCGCTTCCAAAATCCACTCTTACAAATTGAAGGGGTTGGGCGAAAAACTGTCCAAATATTACTTGATAATTTTCAAACAATGCAAAACATTGAAGCTGCTTCTTTGAGCGAATTATCACAGTTTATTCCTGCTAATTTAGCACAAAAAATTAAAACTTTTCTAAAGGAAAATGACTAA", "aa": "MHQQLAQLLQVPSIQSLEIIDISFYDKDHVVGAMLRYENGKRMKALSRRYNINIDHKGDTNYMADVVYRRIISSIQTHKQLPLSDLLIVDGGIAQINTVTKVFASFPNVTQPIIIGLAKNTRHQTDHIVLTDNTTINIDKNTPLFAYLTTIQEEVDSFAKHNAFKRVSRARFQNPLLQIEGVGRKTVQILLDNFQTMQNIEAASLSELSQFIPANLAQKIKTFLKEND", "aa_hexdigest": "89a07411c6a504027f0cf30b313274b6", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF14520.12", "name": "HHH_5", "length": 54, "aa_cov": 0.2236842105263158, "hmm_cov": 1.0, "evalue": 3.6623267018372064e-08, "score": 29.2736759185791, "start": 176, "stop": 226 }, { "id": "PF08459.17", "name": "UvrC_RNaseH_dom", "length": 161, "aa_cov": 0.6710526315789473, "hmm_cov": 0.9751552795031055, "evalue": 5.117553481349761e-34, "score": 112.88227081298828, "start": 7, "stop": 159 } ], "seq_stats": { "molecular_weight": 25918.44150000002, "isoelectric_point": 9.046477699279784 }, "id": "APKECPOBEN_321", "locus": "GCA002090215_00285" }, { "type": "cds", "sequence": "contig_1", "start": 163648, "stop": 164067, "strand": "+", "frame": 1, "gene": "yfcE", "product": "putative metallophosphoesterase MPN_126", "db_xrefs": [ "COG:COG0622", "COG:R", "EC:3.1.4.-", "GO:0016787", "GO:0046872", "KEGG:K07095", "SO:0001217", "UniRef:UniRef50_P47449", "UniRef:UniRef90_P75349" ], "nt": "ATGAAGTTGCACAACCCTGATGTAGTAATTCATGCTGGTGATCATTTAACTACTAAAAAGTTTATGGATCAAAATGCCACTTTTTGGGTTGCTGGTAATCATGATGTTGTTGGCGAAGAGATTCAAATGTTTGAGTTAGAGGGAATCCAGTTTGTTTTAATGCACGGCCATCAAGCGCCCCGTCATGATCTCAAGCAGTGGTATAAAATGCTGGTTGATCAAGCTAAAAGCTATCTTTGTGATGTTTTAATTGTGGGTCACAGTCACATAGAGCACTATGAAACTATTGATGGGATCCAGGTTATTAACCCCGGTTCTCTAGAAATTCCCCGTAATCCGAGAAAGCTACCTACCTACTGTAATTTAAACTTAAGTCAGGGCAGGATAAGTGATTTAACGTTCCATTTTCCTCGGGATTAA", "aa": "MKLHNPDVVIHAGDHLTTKKFMDQNATFWVAGNHDVVGEEIQMFELEGIQFVLMHGHQAPRHDLKQWYKMLVDQAKSYLCDVLIVGHSHIEHYETIDGIQVINPGSLEIPRNPRKLPTYCNLNLSQGRISDLTFHFPRD", "aa_hexdigest": "a7871139f2662e9bb2626c69be59a706", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75349", "query_cov": 1.0, "subject_cov": 0.8742138364779874, "identity": 1.0, "score": 297.0, "evalue": 5.05e-102, "valid": true, "gene": "YfcE", "product": "Probable metallophosphoesterase MPN_126", "ec_ids": [ "3.1.4.-" ], "uniref50_id": "UniRef50_P47449", "cog_id": "COG0622", "cog_category": "R", "kegg_orthology_id": "K07095", "go_ids": [ "GO:0016787", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_P47449", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47449" ], "product": "Probable metallophosphoesterase MG207" }, "genes": [ "yfcE" ], "id": "APKECPOBEN_322", "locus": "GCA002090215_00286" }, { "type": "cds", "sequence": "contig_1", "start": 164516, "stop": 165232, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01519.22", "PFAM:PF23991.1" ], "nt": "GTGACGAACCACCTTCTTAAACATTTTTACGTTTTTCCAATCAATAATAAAGGAGGTCTTGCCATTAAACGCATAATTTCACTTGCTTTGTTTAAGAAAAGACTTAACAAAGATAAGATTAATAATTGTCATGTTTGGGAAGAAGAGTTACCTGATGGTAGCTACGACATGGGATTTAATGGCAATTTCAACCATATGGAAAAACGAAAAAGTGGTTATGTTACCCAAAAGCAGTTTAGCGAGTTCAAAGATGCCAACAATCAGCGTCTCATAAAGATTGAAACTACTTTGGCTATCCAAGGCGAACAAATCAACAAATTGACTCAAACTGTTGAAAAGCAAGGCGAACAAATCAATCAATTAGTTCAAGTTGTGCTTCTTCAGGGCGAGCAAATTAGAGAACTTCAAGTGGAGCAAAAAGCACAAAGACAAGAGTTTAATGCCCGCATGGATCGTTTGGAAAATCTTTTGGTGGAAAGTATAGAATCTACCAATAAGCGCTTCGACTCTATGGAAAGACGTTTAGACTCTATGGATAGTCGTCTTGATTCTATGGAAAATCGCTTAGATTCAATGGAAGGTCGTCTTGATTCTATGGAAAATCGCTTAGATTCAATGGAAGGTCGTCTTGATTCTGTTGAAGGACGCTTAGACTCTATGGAAACTCGTTTAGACTCTATGGAAACTCGCCTGGACAAAGTCGATCCGCCCAAATAG", "aa": "MTNHLLKHFYVFPINNKGGLAIKRIISLALFKKRLNKDKINNCHVWEEELPDGSYDMGFNGNFNHMEKRKSGYVTQKQFSEFKDANNQRLIKIETTLAIQGEQINKLTQTVEKQGEQINQLVQVVLLQGEQIRELQVEQKAQRQEFNARMDRLENLLVESIESTNKRFDSMERRLDSMDSRLDSMENRLDSMEGRLDSMENRLDSMEGRLDSVEGRLDSMETRLDSMETRLDKVDPPK", "aa_hexdigest": "208e442be14448e754e37817ffef02eb", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01519.22", "name": "DUF16", "length": 68, "aa_cov": 0.27310924369747897, "hmm_cov": 1.0, "evalue": 4.203939221232866e-39, "score": 128.7352752685547, "start": 59, "stop": 123 }, { "id": "PF23991.1", "name": "DUF7310", "length": 50, "aa_cov": 0.21008403361344538, "hmm_cov": 1.0, "evalue": 3.7154085548638056e-14, "score": 48.49476623535156, "start": 184, "stop": 233 } ], "seq_stats": { "molecular_weight": 27871.27210000001, "isoelectric_point": 5.7404767990112315 }, "id": "APKECPOBEN_323", "locus": "GCA002090215_00287" }, { "type": "cds", "sequence": "contig_1", "start": 165489, "stop": 165770, "strand": "+", "frame": 3, "gene": null, "product": "Family K-like protein", "db_xrefs": [ "RefSeq:WP_015344878.1", "SO:0001217", "UniParc:UPI0002B84587", "UniRef:UniRef100_A0AAP8E4D3", "UniRef:UniRef50_A0AAP8E4D3", "UniRef:UniRef90_A0AAP8E4D3" ], "nt": "ATGAGTAATACAAAAAAATTAAGAATAACTTCAATCGAAGAAACAATAGAAATAATAGTTAATTCAAAACTATTAGCTTTTGGTTTTTACCCAGTTGGTTTAGAAGATGGAACTTACGAACTTAATGTAAGGATTATTTTTAAAGAAAAAACTGCAGATTCTGAGAAGAGTAGCAGTGTACATCCTTTACAAAAGGAGTATTATGTAAGGGAATTTTTGAGATTTCTTGAGCGCCATAAAGATTCTCTGGCGCAACAACTATTAGTAGTAGAAGACAATTAA", "aa": "MSNTKKLRITSIEETIEIIVNSKLLAFGFYPVGLEDGTYELNVRIIFKEKTADSEKSSSVHPLQKEYYVREFLRFLERHKDSLAQQLLVVEDN", "aa_hexdigest": "6535a3d15727147d2970eafc4bef2012", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0002B84587", "ncbi_nrp_id": "WP_015344878.1", "uniref100_id": "UniRef100_A0AAP8E4D3", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002B84587", "RefSeq:WP_015344878.1", "UniRef:UniRef100_A0AAP8E4D3" ] }, "ips": { "uniref100_id": "UniRef100_A0AAP8E4D3", "uniref90_id": "UniRef90_A0AAP8E4D3" }, "psc": { "uniref90_id": "UniRef90_A0AAP8E4D3", "product": "Family K-like protein", "uniref50_id": "UniRef50_A0AAP8E4D3" }, "pscc": { "uniref50_id": "UniRef50_A0AAP8E4D3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAP8E4D3" ], "product": "Family K-like protein" }, "genes": [], "id": "APKECPOBEN_324", "locus": "GCA002090215_00288" }, { "type": "cds", "sequence": "contig_1", "start": 166023, "stop": 166280, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF12378.14" ], "nt": "GTGCGCGACAACAGCGCTAAGGGCATCACTGCCGGCAGTGGCAGTCAACAAACCACGTATGAACCTGCGCGAACCGAAGCGGCTTTGACCGCATCAACCACCTTTGCGTTACGCCGGTATGACCTCGCCGGGCGCGCCTTATACGACCTCGACTTTTCGAAGTTAAACCCACAAACGCCCACGCGCGACCAAACCGGGCAGATCACCTTTAACCCCTTTGGCGGCTTTGATTTGAGTGGGGCTGCACCCCAACAGTGA", "aa": "MRDNSAKGITAGSGSQQTTYEPARTEAALTASTTFALRRYDLAGRALYDLDFSKLNPQTPTRDQTGQITFNPFGGFDLSGAAPQQ", "aa_hexdigest": "22fd51ce8d343e3cf040f3ab30e17d95", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF12378.14", "name": "P1_N", "length": 58, "aa_cov": 0.6823529411764706, "hmm_cov": 0.896551724137931, "evalue": 9.034138971936787e-09, "score": 30.511953353881836, "start": 27, "stop": 84 } ], "seq_stats": { "molecular_weight": 9132.891699999996, "isoelectric_point": 6.071961784362792 }, "id": "APKECPOBEN_325", "locus": "GCA002090215_00289" }, { "type": "cds", "sequence": "contig_1", "start": 166311, "stop": 166904, "strand": "+", "frame": 3, "gene": null, "product": "putative protein MPN_128", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75347", "UniRef:UniRef90_P75347" ], "nt": "GTGGCGCAAGACCCCTCCAATCCCTACCGGTTTGCCGTTTTACTCGTGCCGCGCAGCGTGGTGTACTATGAGCAGTTGCAAAGGGGGTTGGGCTTACCACAGCAGCGAACCGAGAATGGTCAAAATACTTCCACCACCGGGGCAATGTTTGGCTTGAAGGTGAAGAACGCCGAGGCGGACACCGCGAAGAGCAATGAAAAACTCCAGGGCGCTGAGGCCACTGGTTCTTCAACCACATCTGGATCTGGCCAATCCACCCAACGTGGGGGTTCGTCAGGGGACACCAAAGTCAAGGCGTTGCAGGTGGCGGTGAAAAAGAAATCCGGGAGTCAGGGCAACTCCGGTGACCAAGGCACCGAACAGGTGGAACTTGAATCTAATGATTTAGCCAACGCCCCGATTAAACGGGGCTCCAATCCAGCAAGTCCAACTCAAGGCAGCCGACTTCGGCACCACCCCATCCAGTTCGGAATCTGGTCAATCAGGCACCCCCACCCCCTGAAGGCCGTGGCTTGCGACCGAGCAAATTCACAAGGACCTCCCCAAATGATCCGCCTCGATCCTCATTCTGTACGATGCGCCTTATGCCTTTAA", "aa": "MAQDPSNPYRFAVLLVPRSVVYYEQLQRGLGLPQQRTENGQNTSTTGAMFGLKVKNAEADTAKSNEKLQGAEATGSSTTSGSGQSTQRGGSSGDTKVKALQVAVKKKSGSQGNSGDQGTEQVELESNDLANAPIKRGSNPASPTQGSRLRHHPIQFGIWSIRHPHPLKAVACDRANSQGPPQMIRLDPHSVRCALCL", "aa_hexdigest": "8a2c922db3322e8ec49ba14d74ccd9db", "start_type": "GTG", "rbs_motif": "AACAA", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 166011, "stop": 166904, "observations": { "mutation": [ 166278 ], "stop": [ 166278 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75347", "query_cov": 1.0, "subject_cov": 0.5225464190981433, "identity": 0.99, "score": 377.0, "evalue": 1.47e-129, "gene_start": 1, "gene_end": 197, "reference_start": 181, "reference_end": 377, "reference_sequence": "MISPKLDWLILTATASLAAGLTVVGHFTSTTTTLKRQQFSYTRPDEVALRHTNAINPRLTPWTYRNTSFSSLPLTGENPGAWALVRDNSAKGITAGSGSQQTTYEPARTEAALTASTTFALRRYDLAGRALYDLDFSKLNPQTPTRDQTGQITFNPFGGFGLSGAAPQQWNEVKNKVPVEVAQDPSNPYRFAVLLVPRSVVYYEQLQRELGLPQQRTENGQNTSTTGAMFGLKVKNAEADTAKSNEKLQGAEATGSSTTSGSGQSTQRGGSSGDTKVKALQVAVKKKSGSQGNSGDQGTEQVELESNDLANAPIKRGSNPASPTQGSRLRHHPIQFGIWSIRHPHPLKAVACDRANSQGPPQMIRLDPHSVRCALCL" }, "paralog": false, "identity": 0.98989898989899, "score": 586.0, "evalue": 3.78e-214, "description": "Internal stop codon at 166278. Nonsense mutation around 166278.", "psc": { "uniref90_id": "UniRef90_P75347", "product": "Uncharacterized protein MPN_128", "uniref50_id": "UniRef50_P75347", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75347", "UniRef:UniRef50_P75347" ] }, "pscc": { "uniref50_id": "UniRef50_P75347", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75347" ], "product": "Uncharacterized protein MPN_128" } }, "id": "APKECPOBEN_326", "locus": "GCA002090215_00290" }, { "type": "cds", "sequence": "contig_1", "start": 167604, "stop": 167960, "strand": "+", "frame": 3, "gene": null, "product": "putative protein MPN_129", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P75346", "UniRef:UniRef90_P75346" ], "nt": "ATGGTCTCTTTTTTTGATATATATACCAAAGCATCGATTGTAGAAGCTGTACGTTTTTCTTTTTTGAATACCTTTCAATTAAAGGAACTTGGTTTACCGATTAAAGAAATTGCTGTACCATTAGGCACATTAGTCTTTCTTTTTGTTGTTATTATTACCTTAATTCCGTTGTTAATTATTGGTAATTTAATTTGGACTAACTTGCGTTTAATAGAACGGGAAAATACACAGCAGTACCAACTCGTTTTTGGTTACAGTTTGATAGTTAGTGATATTGTTGGTTTTGCTATTGTTTTCTTCGGTGCCATTTTAGGTACAAATTTAAAAAGTGTTGAATTATTCATAGCATTGGGTTGA", "aa": "MVSFFDIYTKASIVEAVRFSFLNTFQLKELGLPIKEIAVPLGTLVFLFVVIITLIPLLIIGNLIWTNLRLIERENTQQYQLVFGYSLIVSDIVGFAIVFFGAILGTNLKSVELFIALG", "aa_hexdigest": "cd2e740ca967c78b750e6197fc5c17b1", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 167604, "stop": 168053, "observations": { "mutation": [ 167958 ], "stop": [ 167958 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75346", "query_cov": 1.0, "subject_cov": 0.7919463087248322, "identity": 1.0, "score": 214.0, "evalue": 9.18e-70, "gene_start": 1, "gene_end": 118, "reference_start": 1, "reference_end": 118, "reference_sequence": "MVSFFDIYTKASIVEAVRFSFLNTFQLKELGLPIKEIAVPLGTLVFLFVVIITLIPLLIIGNLIWTNLRLIERENTQQYQLVFGYSLIVSDIVGFAIVFFGAILGTNLKSVELFIALGWMMMLGSLIALGTTANLVSSIYLYIKLALKR" }, "paralog": false, "identity": 0.9932885906040269, "score": 278.0, "evalue": 1.79e-97, "description": "Internal stop codon at 167958. Nonsense mutation around 167958.", "psc": { "uniref90_id": "UniRef90_P75346", "product": "Uncharacterized protein MPN_129", "uniref50_id": "UniRef50_P75346", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75346", "UniRef:UniRef50_P75346", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75346", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75346" ], "product": "Uncharacterized protein MPN_129" } }, "id": "APKECPOBEN_327", "locus": "GCA002090215_00291" }, { "type": "cds", "sequence": "contig_1", "start": 168036, "stop": 168443, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W7Y3" ], "nt": "GTGGTTTTCGTCACTTTGTCCGTTCTTATCATTCCTTCACCCCGCGCCGGTTCAGAGCAGGAGGTTGGCTTGGTCGAGGGTGGGTCATTTGGAGCCGCCTGTAGTCGTCTTCTGACTGCCACCAAGCGTGGCTTGGACGTTGGCTTTGAGGCCAACGCCGACATTCGAGAACGTCGGGAGAAAGGAACGCGAACCACTTGTGTTCGTACTAGTTCCGCTGGACGACCCCGCGCCGGTTTTAGGCGCGGTTATATCATCCACGCCCCGGTGGTTATTCCCATACCTGTACCCATTCCCAATCCAGGGCACATAATCCAACACCTGGTCGAGAACCGACCCCGCGAGGTTGAACCCGGTGGAGAAGCGCGTGAGCGTGGGGGAGAAGTGTGTCTATCTCTTCAATGCTAG", "aa": "MVFVTLSVLIIPSPRAGSEQEVGLVEGGSFGAACSRLLTATKRGLDVGFEANADIRERREKGTRTTCVRTSSAGRPRAGFRRGYIIHAPVVIPIPVPIPNPGHIIQHLVENRPREVEPGGEARERGGEVCLSLQC", "aa_hexdigest": "67335385041c51049ca065e41dce58c8", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0027E159D2", "query_cov": 0.9851851851851852, "subject_cov": 0.9432624113475178, "identity": 0.812, "score": 203.0, "evalue": 2.6e-65, "valid": false, "uniref50_id": "UniRef50_A0AB38W7Y3" }, "pscc": { "uniref50_id": "UniRef50_A0AB38W7Y3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W7Y3" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14521.533299999986, "isoelectric_point": 9.238915824890135 }, "id": "APKECPOBEN_328", "locus": "GCA002090215_00292" }, { "type": "cds", "sequence": "contig_1", "start": 169182, "stop": 169436, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01519.22" ], "nt": "GTGAACACGGAATACGTAACGCGTAAAGAGTTCAACGAGTACAAAGACTCAAACGACCAACGACTCATAAAGATTGAAACCACTTTGGCTGCCCAAGGTGAACAAATAAGAATCCAGGGTGAGCAAATTAAAGAACTTCAAATCGAGCAAAAAGCTCAGAGCGAAACCTTAAAGCTAATCTTGCAAACTTTGCAAAAGATGAGCGATCGCTTGGACAAGATGGATGTTCGCCTAGATAAACTAGAGTCTAAGTAG", "aa": "MNTEYVTRKEFNEYKDSNDQRLIKIETTLAAQGEQIRIQGEQIKELQIEQKAQSETLKLILQTLQKMSDRLDKMDVRLDKLESK", "aa_hexdigest": "16b19b2bd79f9e2b79df3e957369e096", "start_type": "GTG", "rbs_motif": "AACAA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01519.22", "name": "DUF16", "length": 88, "aa_cov": 0.8809523809523809, "hmm_cov": 1.0, "evalue": 5.6752278300145454e-27, "score": 89.75855255126953, "start": 2, "stop": 75 } ], "seq_stats": { "molecular_weight": 9957.249299999994, "isoelectric_point": 5.501753044128416 }, "id": "APKECPOBEN_329", "locus": "GCA002090215_00293" }, { "type": "cds", "sequence": "contig_1", "start": 169702, "stop": 169899, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0H3DJX7", "UniRef:UniRef90_A0AAN4XEA6" ], "nt": "ATGAAAAAAGTTAAAGCATTAAGGAATCTTTTTTTAGAAAAAGCCAGTGAAAAACTTGAATACCCCAAAATTGCTTCAATGGTAGCTTACTTTGATCCAAAGGAAGATGGTACTTGTGAATTCATTATGAAAATTAAAACTGTCAATGAAGTTGCTGACACTGAGACGACAAATAAGGAAAAGCCTTTAGGCACTTGA", "aa": "MKKVKALRNLFLEKASEKLEYPKIASMVAYFDPKEDGTCEFIMKIKTVNEVADTETTNKEKPLGT", "aa_hexdigest": "8f5463db56fabcd688e705e868eeecf6", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0AAN4XEA6", "query_cov": 1.0, "subject_cov": 0.8552631578947368, "identity": 0.985, "score": 124.0, "evalue": 3.56e-36, "valid": true, "uniref50_id": "UniRef50_A0A0H3DJX7" }, "pscc": { "uniref50_id": "UniRef50_A0A0H3DJX7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0H3DJX7" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7412.577499999999, "isoelectric_point": 7.800045967102052 }, "id": "APKECPOBEN_330", "locus": "GCA002090215_00294" }, { "type": "cds", "sequence": "contig_1", "start": 170040, "stop": 170297, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002D96482" ], "nt": "GTGTTGGATTATGTGCCCTGGATTGGGAATGGGTACAGGTATGGGAATAACCACCGGGGGAGCAACAGTAGTACGAGTGGGGTAACGACCCAGGGACAATCCCAAAATGCATCCAGTAACGAACCCGCACCGACGTTTTCGAATGTCGGCGTTGGTCTCAAAGCCAATGTCAACGGCACCTTAAGTGGTTCGCGAACTACGCCTAATCAGCAAGGCACTCCCTGGTTAACCCTCGACCAAGCCAACCTCCAGCTCTGA", "aa": "MLDYVPWIGNGYRYGNNHRGSNSSTSGVTTQGQSQNASSNEPAPTFSNVGVGLKANVNGTLSGSRTTPNQQGTPWLTLDQANLQL", "aa_hexdigest": "f682510355e5f0014a5647b598ae3463", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0002D96482", "query_cov": 1.0705882352941176, "subject_cov": 0.8504672897196262, "identity": 0.593, "score": 95.9, "evalue": 5.27e-24, "valid": false, "uniref50_id": "UniRef50_UPI0002D96482" }, "pscc": { "uniref50_id": "UniRef50_UPI0002D96482", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002D96482" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8982.590799999998, "isoelectric_point": 8.209807014465333 }, "id": "APKECPOBEN_331", "locus": "GCA002090215_00295" }, { "type": "cds", "sequence": "contig_1", "start": 170588, "stop": 170911, "strand": "+", "frame": 2, "gene": null, "product": "Adhesin P1-like protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB33HTF6", "UniRef:UniRef90_A0AB33HTF6" ], "nt": "GTGCAGCTGTTCCTGCGCGGCCTGTTGGGCAGCATCCCGGTGTTGGTCAATAAGAGTGGGCAAGATGATAACAGTAAGTTTAATTCCACCGACCAAAAATGGTCTTACACCGAATTAAAGTCGGATCAAACCAAGCTCAACCTCCCCGCTTATGGTGAGGTGAATGGGTTGTTGAATCCGGCGTTGGTGGAAACCTATTTTGGGACGACGCGTACGTCTAGCACTGCGAACCAAAACAGTACAACCGTCCCCGGTATCGGTTTTAAAATTCCCGAACAAAATAATGATTCAAAGGCTGTGCTGATCACCCCCGGGTTGGCTTGA", "aa": "MQLFLRGLLGSIPVLVNKSGQDDNSKFNSTDQKWSYTELKSDQTKLNLPAYGEVNGLLNPALVETYFGTTRTSSTANQNSTTVPGIGFKIPEQNNDSKAVLITPGLA", "aa_hexdigest": "2b410c9e78b5be872c3b5f217872ac63", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 170555, "stop": 171214, "observations": { "mutation": [ 170909, 171053 ], "stop": [ 170909, 171053 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB33HTF6", "query_cov": 1.0, "subject_cov": 0.4863636363636364, "identity": 0.972, "score": 205.0, "evalue": 2.64e-65, "gene_start": 1, "gene_end": 107, "reference_start": 12, "reference_end": 118, "reference_sequence": "MSFTNKNNAQRVQLFLRGLLGSIPVLVNKSGQDDNSKFNSTDQKWSYTELKSDQTKLNLPAYGEVNGLLNPALVETYFGTTLTSSTANQNSTTVPSIGFKIPEQNNDSKAVLITPGLAWTPQDVGNLVVSGTSLSFQLGGWLVTVHGLYQTPRGLFGPPVKWPGCQWQRPKGVNLGQAALSGISWQLGQPVGTGRECVGFEGGVGGSSSQRGVRVASSYK" }, "paralog": false, "identity": 0.9771689497716894, "score": 433.0, "evalue": 1.03e-157, "description": "Internal stop codon at 170909, 171053. Nonsense mutation around 170909, 171053.", "psc": { "uniref90_id": "UniRef90_A0AB33HTF6", "product": "Adhesin P1 homolog", "uniref50_id": "UniRef50_A0AB33HTF6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB33HTF6", "UniRef:UniRef50_A0AB33HTF6" ] }, "pscc": { "uniref50_id": "UniRef50_A0AB33HTF6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB33HTF6" ], "product": "Adhesin P1 homolog" } }, "id": "APKECPOBEN_332", "locus": "GCA002090215_00296" }, { "type": "cds", "sequence": "contig_1", "start": 171126, "stop": 171773, "strand": "+", "frame": 3, "gene": null, "product": "Putative adhesin P1-like protein MPN_132", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75266", "UniRef:UniRef90_P75266" ], "nt": "TTGGGACGGGTCGAGAGTGTGTGGGATTTGAAGGGGGTGTGGGCGGATCAAGCTCACAGCGCGGTGTCCGAGTCGCAAGCAGCTACAAGTAGTACCACCACCACCGCAACAGGGGACACCTTACCGGAGCACCCGAATGCCCTCGCGTACCAAATTAGCTCCACCGACAAGGATTCGTACAAGGCTTCCACTCAAGGCTCCGGTCAAACCAATTCCCAAAACACCTCCCCCTACCTGCATTTGATTAAACCTAAAAAAGTTACTGCTTCCGACAAGTTAGACGACGATCTTAAAAACCTGTTGGACCCCAACGAGGTTCGGGTGAAGCTGCGCCAAAGCTTTGGTACAGACCATTCCACCCAACCCCAACCCCAACCCCTCAAAACAACGACACCGGTGTTTGGGACGAATAGTGGTAACCTCGGTAGTGTGCTTAGTGGTGGGGGTACCACGCAGGACTCAAGCACCACCAATCAACTGTCACCCGTTCAACGGGTGAGTGGGTGGCTTGTGGGGCAGTTACCAAGCACGAGTGACGGAAACACCTCCTCCACCAACAACCTCGCGCCTAATACTAATACGGGGAATGAGGTGGTGGGGGTGGGAGATTTGTCTAAGCGAGCTTCCATTGAATCAAGTCGTTTGTGA", "aa": "MGRVESVWDLKGVWADQAHSAVSESQAATSSTTTTATGDTLPEHPNALAYQISSTDKDSYKASTQGSGQTNSQNTSPYLHLIKPKKVTASDKLDDDLKNLLDPNEVRVKLRQSFGTDHSTQPQPQPLKTTTPVFGTNSGNLGSVLSGGGTTQDSSTTNQLSPVQRVSGWLVGQLPSTSDGNTSSTNNLAPNTNTGNEVVGVGDLSKRASIESSRL", "aa_hexdigest": "0747e18764de320f0e768aff0c58e77c", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75266", "query_cov": 1.0093023255813953, "subject_cov": 0.8410852713178295, "identity": 0.982, "score": 395.0, "evalue": 4.92e-138, "valid": true, "product": "Putative adhesin P1-like protein MPN_132", "uniref50_id": "UniRef50_P75266" }, "pscc": { "uniref50_id": "UniRef50_P75266", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75266" ], "product": "Putative adhesin P1-like protein MPN_132" }, "genes": [], "id": "APKECPOBEN_333", "locus": "GCA002090215_00297" }, { "type": "cds", "sequence": "contig_1", "start": 172249, "stop": 172725, "strand": "+", "frame": 1, "gene": "yncB", "product": "putative lipo MG186-like protein", "db_xrefs": [ "COG:COG1525", "COG:L", "GO:0004519", "GO:0005886", "SO:0001217", "UniRef:UniRef50_P75265", "UniRef:UniRef90_P75265" ], "nt": "ATGAAAGGGTTTTCTTGCTCCAGACCGGGCTATCTTACTGGTCTCTTGCTCCTCGCTGTAGCCCCTATTCTCACTGCTTGTACCCGCGACTATACTACCAAGAACGAATTTCAATTAACAACAGCTCAACAGGCCAAATTAAAGCCAGCCACCATCGAATATTGGCGTGATGGTGATACGCCTGAGATCAATTACGCAAGCGAAGAGCGCCGAAAGGAAGCTGAACAAAAATCTAAAGAAAACGCTAAGAAAGAAGACAAAAAAGAGGAAAAGAAAACTGAAGATTCCCAAGATTCTTCTTCTGCATCCACGCAAGTTCGTAGTAGTAAACACGGTCTCCGCATCTATGGAATAGATACACCCGAAAAACATGTAAGTAGCAAAGGCGATAGCACCGGTGATGAAAAAATCGAAGCCGAAAAGGCCTCTAACTATGCTGAAAAGCTAATCCCTAAAGGCAGTACTGTTTGGGTTTGA", "aa": "MKGFSCSRPGYLTGLLLLAVAPILTACTRDYTTKNEFQLTTAQQAKLKPATIEYWRDGDTPEINYASEERRKEAEQKSKENAKKEDKKEEKKTEDSQDSSSASTQVRSSKHGLRIYGIDTPEKHVSSKGDSTGDEKIEAEKASNYAEKLIPKGSTVWV", "aa_hexdigest": "8111908103a440578a0df78a47b4c3c9", "start_type": "ATG", "rbs_motif": null, "genes": [ "yncB" ], "truncated": "3-prime", "pseudogene": { "start": 172249, "stop": 173028, "observations": { "mutation": [ 172723 ], "stop": [ 172723 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75265", "query_cov": 1.0, "subject_cov": 0.5249169435215947, "identity": 1.0, "score": 257.0, "evalue": 6.1e-84, "gene_start": 1, "gene_end": 158, "reference_start": 1, "reference_end": 158, "reference_sequence": "MKGFSCSRPGYLTGLLLLAVAPILTACTRDYTTKNEFQLTTAQQAKLKPATIEYWRDGDTPEINYASEERRKEAEQKSKENAKKEDKKEEKKTEDSQDSSSASTQVRSSKHGLRIYGIDTPEKHVSSKGDSTGDEKIEAEKASNYAEKLIPKGSTVWVWSLNTYSYDREVGALFFKSNPKQTFFQSFEVAMVEAGHAIPIAGTGLNLIADPELSADDPLSVIGLQLANAANKAYNAKINIWSHDTDGYRSLTAVYKLRGADISWTRFLDEANGYSSASAGTGASLYQLWDQRQAKLAQKGS" }, "paralog": false, "identity": 0.9961389961389961, "score": 458.0, "evalue": 1.75e-165, "description": "Internal stop codon at 172723. Nonsense mutation around 172723.", "psc": { "uniref90_id": "UniRef90_P75265", "gene": "YncB", "product": "Uncharacterized lipoprotein MG186 homolog", "uniref50_id": "UniRef50_P75265", "cog_id": "COG1525", "cog_category": "L", "go_ids": [ "GO:0005886", "GO:0004519" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75265", "UniRef:UniRef50_P75265", "COG:COG1525", "COG:L", "GO:0005886", "GO:0004519" ] }, "pscc": { "uniref50_id": "UniRef50_P75265", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75265" ], "product": "Uncharacterized lipoprotein MG186 homolog" } }, "id": "APKECPOBEN_334", "locus": "GCA002090215_00298" }, { "type": "cds", "sequence": "contig_1", "start": 172756, "stop": 173040, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGGCGCTTTATTCTTTAAGTCTAACCCTAAGCAAACCTTCTTCCAATCCTTTGAAGTAGCTATGGTTGAGGCTGGTCATGCTATTCCAATTGCTGGGACTGGTTTAAATTTAATCGCTGATCCGGAATTAAGTGCTGATGATCCTTTAAGTGTCATTGGTCTACAGTTAGCCAACGCTGCCAATAAGGCTTACAACGCTAAAATTAACATTTGGAGCCATGACACTGATGGTTATCGTTCCTTGACTGCTGTTTACAAGTTAAGGGGCGCTGATATTAGTTGA", "aa": "MGALFFKSNPKQTFFQSFEVAMVEAGHAIPIAGTGLNLIADPELSADDPLSVIGLQLANAANKAYNAKINIWSHDTDGYRSLTAVYKLRGADIS", "aa_hexdigest": "c9560ff980c34fa65a91b6462f8af8b7", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10070.29969999999, "isoelectric_point": 5.466001319885254 }, "id": "APKECPOBEN_335", "locus": "GCA002090215_00299" }, { "type": "cds", "sequence": "contig_1", "start": 173178, "stop": 173522, "strand": "+", "frame": 3, "gene": null, "product": "ABC transporter ATP-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAU7Q6T1" ], "nt": "ATGGAAAAGATACAAGCGGAAAAATCCCAAAGTGCAATTGAGTTTAAGAATATTGTAGTCGACTTTGGTGAGTCAATCGCAATTGATAACATTAACCTCACGGTTAAAAAGAAGGAGTTAGTTACCTTACTAGGTCCTTCTGGGTGTGGGAAAACAACTTCGCTTTCCGTGATTGCGGGTCTTATCGCCCCTACTAGTGGTCAGGTTTTATTTAATGGTTATGATGTCACAAAAAAGCCACCACAACAACGCAAACTGGGATTAGTGTTCCAAAACTATGCCTTGTATCCGCACATGAGTGTGTTTGAAAACATTGTCTTCCCACTCTACAGTGATACGTCTTGA", "aa": "MEKIQAEKSQSAIEFKNIVVDFGESIAIDNINLTVKKKELVTLLGPSGCGKTTSLSVIAGLIAPTSGQVLFNGYDVTKKPPQQRKLGLVFQNYALYPHMSVFENIVFPLYSDTS", "aa_hexdigest": "875cdab9b3219eea9ca66ce6958763fc", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0AAU7Q6T1", "query_cov": 0.8859649122807017, "subject_cov": 0.8938053097345132, "identity": 0.505, "score": 116.0, "evalue": 1.69e-31, "valid": false, "product": "ABC transporter ATP-binding protein", "uniref50_id": "UniRef50_A0AAU7Q6T1" }, "pscc": { "uniref50_id": "UniRef50_A0AAU7Q6T1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAU7Q6T1" ], "product": "ABC transporter ATP-binding protein" }, "genes": [], "id": "APKECPOBEN_336", "locus": "GCA002090215_00300" }, { "type": "cds", "sequence": "contig_1", "start": 173574, "stop": 173942, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATCTTAAAGGCTAATGGTGCTACTAGTGAGGAGCTGGCAGAGCTCAATCGTTTAATGCAACAACGAATCGATGAGCCCAAACGAATGGCTTACCAGATTAATGATTTGATGGTAAGTGTGTTTCAAAAACAGAGTGAACTGGAAGCTAACCTAAAGTTAATTCCCCGCAAAAAACAGTTTGCCATTATTTCACTCAGCAAGGAAACACTAAGTCAAATAAGGGATGTTGAAACTAAAGCCAAGGCTGCATTGGAAACCGCTGATAGTGCTGAAGTGGAGCAGACCATTAAAAGTGAACTTAAGCAAAAACTAAGTGAAATTAAGGCTAATTACCACGATGAAAAGGCCAACATTAAAGCTTACTGA", "aa": "MILKANGATSEELAELNRLMQQRIDEPKRMAYQINDLMVSVFQKQSELEANLKLIPRKKQFAIISLSKETLSQIRDVETKAKAALETADSAEVEQTIKSELKQKLSEIKANYHDEKANIKAY", "aa_hexdigest": "8e8b9dee8047eaeb172646f3bb40250d", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13922.866499999982, "isoelectric_point": 7.878310585021973 }, "id": "APKECPOBEN_337", "locus": "GCA002090215_00301" }, { "type": "cds", "sequence": "contig_1", "start": 173949, "stop": 174038, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGGCCAATATTAAGACCGAATTAAAAACCGAAAAAACTGCCATTAAGCAAACTAATGACTATGCCAAATTGAAGGAATTGAAGTGA", "aa": "MLANIKTELKTEKTAIKQTNDYAKLKELK", "aa_hexdigest": "e1eaa73ae50a081dc00917ea6413f12d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3364.949300000001, "isoelectric_point": 9.523737144470214 }, "id": "APKECPOBEN_338", "locus": "GCA002090215_00302" }, { "type": "cds", "sequence": "contig_1", "start": 174057, "stop": 174626, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00005.33" ], "nt": "TTGAATTTAAAGAAGCAGTACCGCAGTTACTTTAAACAACTAAAAGCCAAATACAGCTTAAAAGATGGCAATTTAACTGAAAGCGAATTAAGTCAAATTGAGGAGTTGCAAAAACGGATTGTTTCGCTCAAAGATTTCATTAACCGCACTGCTAAAGAAGTAGCTGAAAAACTGGAAATTACTAAGATTCTCCACAAGCGCCCCGCTAATATTTCTGGGGGTCAACAACAACGGGTAGCTATTGCTAGAGCAATTGTTAGAAGACCTAAGGTTCTACTTATGGACGAACCGTTGTCCAACTTAGATGCGAAGTTGCGGGTACAAACACGGCAATGGATCCGTAAGTTCCAGCAAGACCTGCAAATTACTACTGTCTTTGTGACTCACGATCAGGAAGAGGCAATGAGTATTAGCGACACGATTGTCTGCATGTCAACTGGTAAGGTACAGCAAATAGGCTCCCCTTCCGAGTTGTACTTAAAACCGGCCAATGAGTTCGTCGCTACCTTTTTGGGTAGCCCGGAAATGAATATCGTCAACGCTACGGTTAAAGCTGGACAGCTGTTGTGA", "aa": "MNLKKQYRSYFKQLKAKYSLKDGNLTESELSQIEELQKRIVSLKDFINRTAKEVAEKLEITKILHKRPANISGGQQQRVAIARAIVRRPKVLLMDEPLSNLDAKLRVQTRQWIRKFQQDLQITTVFVTHDQEEAMSISDTIVCMSTGKVQQIGSPSELYLKPANEFVATFLGSPEMNIVNATVKAGQLL", "aa_hexdigest": "fc9d91ecccb12c6f47af9d5cf661e7e6", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00005.33", "name": "ABC_tran", "length": 57, "aa_cov": 0.2804232804232804, "hmm_cov": 1.0, "evalue": 1.2231769909126159e-14, "score": 50.5290412902832, "start": 46, "stop": 98 } ], "seq_stats": { "molecular_weight": 21531.76039999999, "isoelectric_point": 9.680137443542481 }, "id": "APKECPOBEN_339", "locus": "GCA002090215_00303" }, { "type": "cds", "sequence": "contig_1", "start": 174756, "stop": 174938, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGAAAACCTAGGGAAACACATGGTTGGGGTTGTGGAGAGCAATGGGGTACAGCTCAATGTGCGCTTAGAATTGAGCCACCAGTTTGAAGTAGGTAATGCAGTTAAGTTTACGATTAAGCCTAATGGCTTACACTTCTTTGATCCCCAAACAACGCAACGGGTGGAGGTAAAACATGTTTAA", "aa": "MENLGKHMVGVVESNGVQLNVRLELSHQFEVGNAVKFTIKPNGLHFFDPQTTQRVEVKHV", "aa_hexdigest": "d0b0d10ab17a162e6af31251d2a1c603", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6784.6942, "isoelectric_point": 6.901981544494629 }, "id": "APKECPOBEN_340", "locus": "GCA002090215_00304" }, { "type": "cds", "sequence": "contig_1", "start": 175021, "stop": 175305, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTGTTTTGCCCAGCTTTATTAACTACCTTTCTCTTTACTTTAGTACCGTTCTTCCTTACGTTACAGAAAGGGTTTAGTCACAATGAGGACATCTACCGGGTTGATTCCCAACAGTTTGGCTTTCAAACTTTCGCTAACCTTTTTAGTGAGTCCAACTTTATTTTGGGCTTGCGCAATTCCTTTTTGTACTCGATTATTTCCCTACCACTGACAATTGTGCTAGCAATTATTATTTCTAGTGCCATTGTCTTTGTGTACCGGAAGTTAGCACGGGGCTTTTGA", "aa": "MLFCPALLTTFLFTLVPFFLTLQKGFSHNEDIYRVDSQQFGFQTFANLFSESNFILGLRNSFLYSIISLPLTIVLAIIISSAIVFVYRKLARGF", "aa_hexdigest": "c7d05f9b2e85e6f87ed8946a8956561a", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10754.560499999989, "isoelectric_point": 9.032939338684084 }, "id": "APKECPOBEN_341", "locus": "GCA002090215_00305" }, { "type": "cds", "sequence": "contig_1", "start": 175372, "stop": 175866, "strand": "+", "frame": 1, "gene": "ugpA", "product": "putative ABC transporter permease MG188-like protein", "db_xrefs": [ "COG:COG1175", "COG:G", "GO:0005886", "GO:0055085", "SO:0001217", "UniRef:UniRef50_P75263", "UniRef:UniRef90_P75263" ], "nt": "TTGGACTCCTCTTCGGGAATCTTAAACAACATCTTTCATGTCAACATTAAGTGGTTAGATTCGGGGGAACGTGATACCTTTAATGCCTTATGGGGAATTCTTATCTTTGGAATTTGGAAGAACATGGCTTTTAATGTGCTGGTAATATCAACCGCCATGTTAAGTGTGGATCCTACTTTATATAAGGTAGCTAACTTAGATGGTGCAAAACCAATTAGGCAGTTCTTTAAGATTACCTTACCTTCAATCCGCCCAACCTTAATTTTTCTGTTGACCCTGTTAATTTTGGGCGGAATGCAGGTGTTTCCAATTTCGTTGTTTAACGGGAATGATAGTGAAGCTGTAACCAATGGGGGTTCGACCATCTTGCTTTACATCTTCCAAAAGATTCGTGATCAGAATAACAATTTTGCTGGTGCTGCTACCTTGGTGTTGTTCATCTTGGGGGTTTGTTATGGGTTAGTGTTGCGCAATGGTTTTCGCTTAATTGAATGA", "aa": "MDSSSGILNNIFHVNIKWLDSGERDTFNALWGILIFGIWKNMAFNVLVISTAMLSVDPTLYKVANLDGAKPIRQFFKITLPSIRPTLIFLLTLLILGGMQVFPISLFNGNDSEAVTNGGSTILLYIFQKIRDQNNNFAGAATLVLFILGVCYGLVLRNGFRLIE", "aa_hexdigest": "d7c01eccbfee5aff83d8ab306d5babb7", "start_type": "TTG", "rbs_motif": null, "genes": [ "ugpA" ], "truncated": "both", "pseudogene": { "start": 175072, "stop": 175920, "observations": { "mutation": [ 175303, 175864, 175873 ], "stop": [ 175303, 175864, 175873 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75263", "query_cov": 1.0, "subject_cov": 0.49848024316109424, "identity": 0.9940000000000001, "score": 323.0, "evalue": 2.94e-109, "gene_start": 1, "gene_end": 164, "reference_start": 148, "reference_end": 311, "reference_sequence": "MFKWLLKHRSKPTPLELGIFDQKVSFWKPFLLFCPALLTTFLFTLVPFFLTLQKGFSHNEDIYRVDSQQFGFQTFANLFSESNFILGLRNSFLYSIISLPLTIVLAIIISSAIVFVYRKLARGFWQTVFFLPYVTSGVAVSIAFIYILDSSSGILNNIFHVNIKWLDSGERDTFNALWGILIFGIWKNMAFNVLVISTAMLSVDPTLYKVANLDGAKPIRQFFKITLPSIRPTLIFLLTLLILGGMQVFPISLFNGNDSEAVTNGGSTILLYIFQKIRDQNNNFAGAATLVLFILGVCYGLVLRNGFRLIEWAQWKIKRHYVQTKLNLV" }, "paralog": false, "identity": 0.9893617021276596, "score": 541.0, "evalue": 1.16e-197, "description": "Internal stop codon at 175303, 175864, 175873. Nonsense mutation around 175303, 175864, 175873.", "psc": { "uniref90_id": "UniRef90_P75263", "gene": "UgpA", "product": "Probable ABC transporter permease protein MG188 homolog", "uniref50_id": "UniRef50_P75263", "cog_id": "COG1175", "cog_category": "G", "go_ids": [ "GO:0005886", "GO:0055085" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75263", "UniRef:UniRef50_P75263", "COG:COG1175", "COG:G", "GO:0005886", "GO:0055085" ] }, "pscc": { "uniref50_id": "UniRef50_P75263", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75263" ], "product": "Probable ABC transporter permease protein MG188 homolog" } }, "id": "APKECPOBEN_342", "locus": "GCA002090215_00306" }, { "type": "cds", "sequence": "contig_1", "start": 175988, "stop": 176278, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTGGGTTTAATCTTTAAAACGGTGGTGTTGTGCTTCTTTGGTTTAATGGTCATCTTTCCCTTTTATTTGATGCTGGTGGTAGCACTCACCAGTGATGAAGTGGTCTTAAACATTCGTGAACCAATTCTCAAATCCGATGGGTGGCACTTCGAGAACTTCCGCCGTGTTTTGGAAGATGGTAAATACCTTAATGCCATTTGGATTAACAGCTTAGTAACAATTTTGTCGATTATCCTACGGCTTTTCTTTACCGTTAGCATGGGCTATGCCTTTTCGTTAAAAAAGTGA", "aa": "MLGLIFKTVVLCFFGLMVIFPFYLMLVVALTSDEVVLNIREPILKSDGWHFENFRRVLEDGKYLNAIWINSLVTILSIILRLFFTVSMGYAFSLKK", "aa_hexdigest": "5766d4828d1bc3b61aa50077b9d04042", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11140.34179999999, "isoelectric_point": 8.888788032531739 }, "id": "APKECPOBEN_343", "locus": "GCA002090215_00307" }, { "type": "cds", "sequence": "contig_1", "start": 176383, "stop": 176571, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGTACTACGTACCATTGGAAAGGCAATTTTGAGGAAGTACCTCAACCCTGTACAACCATCAACAAAGGCCGATTCTTTAATTCGTGGTGGAATTGCTTCAAAGGCAGTACGGAACATAAACCCAGAGAACACAGAAGCGACAAATGGCATTGTTAAGCCCAAGATAATCGCTGGTTTTTCTGGTTGA", "aa": "MVLRTIGKAILRKYLNPVQPSTKADSLIRGGIASKAVRNINPENTEATNGIVKPKIIAGFSG", "aa_hexdigest": "9574a1f301bef378bdbcaa5b0e7ef95f", "start_type": "GTG", "rbs_motif": "AAAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6589.668500000002, "isoelectric_point": 10.690099143981932 }, "id": "APKECPOBEN_344", "locus": "GCA002090215_00308" }, { "type": "cds", "sequence": "contig_1", "start": 176630, "stop": 176851, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTACTAACAAACAGACCCGATCTCAACATTAACCTGTGGGTGTTGGCGCAAGGGACTGATGGCAATGCCGGGCAAAGTGACGAACAAATCCGGGTGTTGTTGAATTTAAAGATGGCAGCAGCCATTTTAGCAATTCTGCCAATGTTTATTGTTTACTTCTTGTTTAGGAAAAGGATTATGAAAGCCGTGGGATCACGTGCCAATACGATTAAAGGATAG", "aa": "MLLTNRPDLNINLWVLAQGTDGNAGQSDEQIRVLLNLKMAAAILAILPMFIVYFLFRKRIMKAVGSRANTIKG", "aa_hexdigest": "f555fe81ac1cf6d78dcb039d05c4ddb1", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8129.638099999993, "isoelectric_point": 10.519902610778807 }, "id": "APKECPOBEN_345", "locus": "GCA002090215_00309" }, { "type": "cds", "sequence": "contig_1", "start": 177429, "stop": 177965, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01519.22", "PFAM:PF23991.1", "PFAM:PF03670.18" ], "nt": "GTGACTAAAAAACCAAGCACAAAGTACGTAACGCATAAACAGCTTGATCAGAAATTAAAAGAGTTCAAGCAAGATCTAATGGTTGAACTGCATGACACATTTGCTACAAAAGCTGACCTTAGAGATTCGGAGGCACGCATTAACCAAAAGTTAGAAGCCTTAGTTCAAGTTGTGCTTCTTCACGGTGAGCAAATCAACAAATTAACTCAAATTGTTGAAAAACAGGGCGAGCAAATTAGAGAACTTCAAGTGGAGCAAAAAGCGCAAAGACAAGAGTTTAACGCCCGCATGGATCGTTTGGAAAACCTTTTGGTTGAAAGTATAGAATCCACCAATAAACGTTTCGACTCTATTGAAGGTCGTCTAGACTCTATGGATAGTCGTCTTGATTCTATGGAAAATCGCTTGGATTCAATAGAAGGTCGCCTTGATTCTGTTGAAGGTCGCCTTGATTCTGTTGAAGGACGTTTAGACTCTATGGAAAATCGCTTGGATTCAATGGAAACTCGCTTAGACAAAATCGATCCACCCAAATAG", "aa": "MTKKPSTKYVTHKQLDQKLKEFKQDLMVELHDTFATKADLRDSEARINQKLEALVQVVLLHGEQINKLTQIVEKQGEQIRELQVEQKAQRQEFNARMDRLENLLVESIESTNKRFDSIEGRLDSMDSRLDSMENRLDSIEGRLDSVEGRLDSVEGRLDSMENRLDSMETRLDKIDPPK", "aa_hexdigest": "e32fc301be21af0e48ae0dd031c74885", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01519.22", "name": "DUF16", "length": 122, "aa_cov": 0.6741573033707865, "hmm_cov": 0.7868852459016393, "evalue": 4.981744003425199e-32, "score": 106.00624084472656, "start": 1, "stop": 120 }, { "id": "PF23991.1", "name": "DUF7310", "length": 80, "aa_cov": 0.43258426966292135, "hmm_cov": 0.9875, "evalue": 5.932134410715175e-13, "score": 44.63884353637695, "start": 96, "stop": 172 }, { "id": "PF03670.18", "name": "UPF0184", "length": 34, "aa_cov": 0.19101123595505617, "hmm_cov": 1.0, "evalue": 2.9088027990794615e-06, "score": 23.109989166259766, "start": 111, "stop": 144 } ], "seq_stats": { "molecular_weight": 20755.202100000006, "isoelectric_point": 5.170609092712401 }, "id": "APKECPOBEN_346", "locus": "GCA002090215_00310" }, { "type": "cds", "sequence": "contig_1", "start": 178364, "stop": 178696, "strand": "-", "frame": 3, "gene": "envC", "product": "UPF0134 protein MPN_138", "db_xrefs": [ "COG:COG4942", "COG:D", "SO:0001217", "UniRef:UniRef50_P75254", "UniRef:UniRef90_P75260" ], "nt": "GTGAACACGGAATACGTAACGCGCAAAGAGTTTAACGAGTACAAAGACTCAAACGACCAACGACTTACAAAGATTGAAAATAAGGTTGATAAGCTGGAAGTTAAGGTCGATAAATTAGAAGAGAAAGTCGATAAGCTAGAAGCTAAAGTGGACAAATTAGAAGAAAAAGTCGACAAACTTGAAGCTAAAGTGGACAAATTAGAAGAAAAAGTCGACAAACTTGAAGCTAAAGTGGACAGTGGCTTTGAAATGTTAGCTAAAATCCTTGCTGCTATTAACAAACGCCTTGATTCTATTGAAGGTCGCTTAGACAAAATTGAACCACCCAAATAG", "aa": "MNTEYVTRKEFNEYKDSNDQRLTKIENKVDKLEVKVDKLEEKVDKLEAKVDKLEEKVDKLEAKVDKLEEKVDKLEAKVDSGFEMLAKILAAINKRLDSIEGRLDKIEPPK", "aa_hexdigest": "70f646a993d663da863ed09bf6473e87", "start_type": "GTG", "rbs_motif": "AACAA", "genes": [ "envC" ], "truncated": "3-prime", "pseudogene": { "start": 178364, "stop": 178864, "observations": { "mutation": [ 178783 ], "stop": [ 178783 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75260", "query_cov": 1.0, "subject_cov": 0.6626506024096386, "identity": 0.991, "score": 105.0, "evalue": 1.62e-26, "gene_start": 1, "gene_end": 110, "reference_start": 57, "reference_end": 166, "reference_sequence": "MKEKIPFYNEKEFNEMMKKTKKGTFSGWYIINPENKSVEFSGNFNRQFKLNKPVIPVNTEYVTRKEFNEYKDSNDQRLTKIENKVDKLEVKVDKLEEKVDKLEAKVDKLEEKVDKLEAKVDKLEEKVDKLEAKVDSGFEMLAKILAAINKRLDSIEGRLDKIEPPK" }, "paralog": false, "identity": 0.9939759036144579, "score": 222.0, "evalue": 4.6e-75, "description": "Internal stop codon at 178783. Nonsense mutation around 178783.", "psc": { "uniref90_id": "UniRef90_P75260", "gene": "EnvC", "product": "UPF0134 protein MPN_138", "uniref50_id": "UniRef50_P75254", "cog_id": "COG4942", "cog_category": "D", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75260", "UniRef:UniRef50_P75254", "COG:COG4942", "COG:D" ] }, "pscc": { "uniref50_id": "UniRef50_P75254", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75254" ], "product": "UPF0134 protein MPN_524" } }, "id": "APKECPOBEN_347", "locus": "GCA002090215_00311" }, { "type": "cds", "sequence": "contig_1", "start": 179101, "stop": 179424, "strand": "-", "frame": 1, "gene": null, "product": "DUF16 family-like protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W8A6" ], "nt": "GTGAACACAGAATACGTAACGCGTAAAGAATTTAACGAGTACAAAGACTCAAACGACCAAAGACTTACAAAGATTGAAACTGAGTTAAAGAGTCAGGGTCAAAGGATTCAAGTTGTTGAGGACAAAGTTGATCGACTAACTGAAGTTGTTATGGTTCAGAGTCAACAAATCAAAACCCAGGGTGAAGCAATGAACGCCCGCATGGATCGCTTTGAAAGTCTTGTGCTTAAAAGCCTAGAATCAATCGGAAACACTTTGACTGATTTTGGTAAACGCCTCGACGTAATAGAAACTCGTTTAGACAAAATCGATCCGCCTAAATAA", "aa": "MNTEYVTRKEFNEYKDSNDQRLTKIETELKSQGQRIQVVEDKVDRLTEVVMVQSQQIKTQGEAMNARMDRFESLVLKSLESIGNTLTDFGKRLDVIETRLDKIDPPK", "aa_hexdigest": "3c068abf02e9b0cbf2163881b33fa05d", "start_type": "GTG", "rbs_motif": "AACAA", "psc": { "uniref90_id": "UniRef90_A0AB38W8A6", "query_cov": 0.9906542056074766, "subject_cov": 0.954954954954955, "identity": 0.613, "score": 101.0, "evalue": 7.27e-26, "valid": false, "product": "DUF16 family-like protein", "uniref50_id": "UniRef50_A0AB38W8A6" }, "pscc": { "uniref50_id": "UniRef50_A0AB38W8A6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W8A6" ], "product": "DUF16 family-like protein" }, "genes": [], "id": "APKECPOBEN_348", "locus": "GCA002090215_00312" }, { "type": "cds", "sequence": "contig_1", "start": 179843, "stop": 180046, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAGCCAAGTACACCGCAAGGGCTCTATTGCAGAAGCAGTTAGTGCCATCCAAGCTCATGATAAGATCGTGATCTTCCACCACATTCGTCCTGATGGCGATTGTTTGGGCGCACAACACGGCTTAGCGCGTTTAATCCAAACTAACTTTCCCCACAAGCAGGTCTTCTGTGTTGGTGATCCCAAACACAACTTTCCCTGA", "aa": "MNSQVHRKGSIAEAVSAIQAHDKIVIFHHIRPDGDCLGAQHGLARLIQTNFPHKQVFCVGDPKHNFP", "aa_hexdigest": "34d9eb2d6a77d3af142893ad558c069f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7434.424999999999, "isoelectric_point": 8.807944679260256 }, "id": "APKECPOBEN_349", "locus": "GCA002090215_00313" }, { "type": "cds", "sequence": "contig_1", "start": 180053, "stop": 180385, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01368.27" ], "nt": "ATGGTTTTCACTCCAAAGGAACAGATTACCCCGGAGTTAATGCAACAAGCCTTAGCCGTTATTGTTGATGCCAACTATAAGGAACGGATTGAGTGCCGGGACTTATTAGACCAAAACCAGTTTAAGGCAGTATTGCGGATTGACCACCACCCCAATGAGGACGATCTCAATACGACCCATAACTTCGTTGATGCGTCTTACATTGCCGCGGCTGAACAAGTGGTAGATCTAGCGGTGCAGGCCAAATGGAAGCTTAGCCCCCCAGCGGCTACGGCGCTGTATCTAGGTATTTATACAGATAGTAATAGGTTTCTATATAGTAATACATCATGA", "aa": "MVFTPKEQITPELMQQALAVIVDANYKERIECRDLLDQNQFKAVLRIDHHPNEDDLNTTHNFVDASYIAAAEQVVDLAVQAKWKLSPPAATALYLGIYTDSNRFLYSNTS", "aa_hexdigest": "8f0850adfe18df42c267cf5894436a9b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01368.27", "name": "DHH", "length": 90, "aa_cov": 0.7454545454545455, "hmm_cov": 0.9555555555555556, "evalue": 5.995346258035335e-08, "score": 28.507266998291016, "start": 16, "stop": 97 } ], "seq_stats": { "molecular_weight": 12483.917699999985, "isoelectric_point": 4.811841392517089 }, "id": "APKECPOBEN_350", "locus": "GCA002090215_00314" }, { "type": "cds", "sequence": "contig_1", "start": 180407, "stop": 180817, "strand": "+", "frame": 2, "gene": "nrnA", "product": "Bifunctional oligoribonuclease and PAP phosphatase NrnA", "db_xrefs": [ "COG:COG0618", "COG:FT", "EC:3.1.-.-", "GO:0003676", "GO:0004527", "GO:0008441", "KEGG:K06881", "SO:0001217", "UniRef:UniRef50_P75144", "UniRef:UniRef90_P75144" ], "nt": "ATGCTATATAGAGCTCAAGCTAATATAGCTAAGATCCATGATGAGTTAAACCACACTTCCTTAAAGGACATCCAGTTTAAACAATATGTCTTTAAAAACTTTCAGACCTTTCAGAATGTTATTTACTTTGTGGCCGATAAGAAGTTCCAAAAGAAATTAAAGGTAACACCCTTAGAATGTGCACGGGTAAATATCCTAGCTAACATTGAACAATTCCACATTTGGCTGTTCTTTATAGAAGAGGGTAAGAACCACTATCGGGTCGAATTCCGTAGTAACGGAATTAACGTACGCGAAGTAGCTTTAAAGTATGGTGGCGGGGGTCATATTCAGGCCAGCGGTGCAGTTCTTAAAAGCAAGCGCGACATAATTCGTGTAGTTCAAGATTGCCAAAAGCAAATTGCTGTATAA", "aa": "MLYRAQANIAKIHDELNHTSLKDIQFKQYVFKNFQTFQNVIYFVADKKFQKKLKVTPLECARVNILANIEQFHIWLFFIEEGKNHYRVEFRSNGINVREVALKYGGGGHIQASGAVLKSKRDIIRVVQDCQKQIAV", "aa_hexdigest": "6fdbde93fb658b294c76837d7d885a14", "start_type": "ATG", "rbs_motif": null, "genes": [ "nrnA" ], "truncated": "5-prime", "pseudogene": { "start": 180107, "stop": 180817, "observations": { "mutation": [ 180383 ], "stop": [ 180383 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75144", "query_cov": 1.0, "subject_cov": 0.41975308641975306, "identity": 1.0, "score": 276.0, "evalue": 2.68e-91, "gene_start": 1, "gene_end": 136, "reference_start": 189, "reference_end": 324, "reference_sequence": "MNSQVHRKGSIAEAVSAIQAHDKIVIFHHIRPDGDCLGAQHGLARLIQTNFPHKQVFCVGDPKHNFPWLEMVFTPKEQITPELMQQALAVIVDANYKERIECRDLLDQNQFKAVLRIDHHPNEDDLNTTHNFVDASYIAAAEQVVDLAVQAKWKLSPPAATALYLGIYTDSNRFLYSNTSWRTLYLGSMLYRAQANIAKIHDELNHTSLKDIQFKQYVFKNFQTFQNVIYFVADKKFQKKLKVTPLECARVNILANIEQFHIWLFFIEEGKNHYRVEFRSNGINVREVALKYGGGGHIQASGAVLKSKRDIIRVVQDCQKQIAV" }, "paralog": false, "identity": 0.9957627118644068, "score": 471.0, "evalue": 1.63e-170, "description": "Internal stop codon at 180383. Nonsense mutation around 180383.", "psc": { "uniref90_id": "UniRef90_P75144", "gene": "nrnA", "product": "Bifunctional oligoribonuclease and PAP phosphatase NrnA", "ec_ids": [ "3.1.-.-" ], "uniref50_id": "UniRef50_P75144", "cog_id": "COG0618", "cog_category": "FT", "kegg_orthology_id": "K06881", "go_ids": [ "GO:0008441", "GO:0004527", "GO:0003676" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75144", "EC:3.1.-.-", "UniRef:UniRef50_P75144", "COG:COG0618", "COG:FT", "KEGG:K06881", "GO:0008441", "GO:0004527", "GO:0003676" ] }, "pscc": { "uniref50_id": "UniRef50_P75144", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75144" ], "product": "Bifunctional oligoribonuclease and PAP phosphatase NrnA" } }, "id": "APKECPOBEN_351", "locus": "GCA002090215_00315" }, { "type": "cds", "sequence": "contig_1", "start": 180830, "stop": 181036, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F17E6AB", "UniRef:UniRef90_UPI001F17E6AB" ], "nt": "ATGCACCAAACCAAAAAAACTGCCTTGTCCAAGTCCACTTGGATTCTCATCCTCACCGCCACCGCCTCCCTCGCGACGGGACTCACCGTAGTGGGACACTTCACAAGTACCACCACGACGCTCAAGCGCCAGCAATTTAGCTACACCCGCCCTGACGAGGTCGCGCTGCGCCACACCAATGCCATCAACCCGCGCTTAACCCCGTGA", "aa": "MHQTKKTALSKSTWILILTATASLATGLTVVGHFTSTTTTLKRQQFSYTRPDEVALRHTNAINPRLTP", "aa_hexdigest": "dd9509ba22f1693d7bc861382a14f493", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001F17E6AB", "query_cov": 0.8823529411764706, "subject_cov": 0.8695652173913043, "identity": 0.983, "score": 114.0, "evalue": 3.08e-32, "valid": true, "uniref50_id": "UniRef50_UPI001F17E6AB" }, "pscc": { "uniref50_id": "UniRef50_UPI001F17E6AB", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F17E6AB" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7524.573299999999, "isoelectric_point": 11.0533784866333 }, "id": "APKECPOBEN_352", "locus": "GCA002090215_00316" }, { "type": "cds", "sequence": "contig_1", "start": 181103, "stop": 181360, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF12378.14" ], "nt": "GTGCGCGACAACAGCGCTAAGGGCATCACTGCCGGCAGTGGCAGTCAACAAACCACGTATGATCCCACCCGAACCGAAGCGGCTTTGACCGCATCAACCACCTTTGCGTTACGCCGGTATGACCTCGCCGGGCGCGCCTTATACGACCTCGATTTTTCGAAGTTAAACCCGCAAACGCCCACGCGCGACCAAACCGGGCAGATCACCTTTAACCCCTTTGGCGGCTTTGGTTTGAGTGGGGCTGCACCCCAACAGTGA", "aa": "MRDNSAKGITAGSGSQQTTYDPTRTEAALTASTTFALRRYDLAGRALYDLDFSKLNPQTPTRDQTGQITFNPFGGFGLSGAAPQQ", "aa_hexdigest": "e38d0c68fd329b9f24cab5f38593397d", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF12378.14", "name": "P1_N", "length": 58, "aa_cov": 0.6823529411764706, "hmm_cov": 0.896551724137931, "evalue": 2.229525265858222e-09, "score": 32.50608444213867, "start": 27, "stop": 84 } ], "seq_stats": { "molecular_weight": 9090.854999999996, "isoelectric_point": 8.137860298156738 }, "id": "APKECPOBEN_353", "locus": "GCA002090215_00317" }, { "type": "cds", "sequence": "contig_1", "start": 181391, "stop": 181888, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001375E5B8" ], "nt": "GTGGCGCAAGACCCCTCCAATCCCTACCGGTTTGCCGTTTTACTCGTGCCGCGCAGCGTGGTGTACTATGAGCAGTTGCAAAGGGGGTTGGGCTTACCACAGCAGCGAACCGAGAGTGGTCAAAATACTTCCACCACCGGGGCAATGTTTGGCTTGAAGGTGAAGAACGCCGAGGCGGACACCGCGAAGAGCAATGAAAAACTCCAGGGCGCTGAGGCCACTGGTTCTTCAACCACATCTGGATCTGGCCAATCCACCCAACGTGGGGGTTCGTCAGGGGACACCAAAGTCAAGGCTTTAAAAATAGAGGTGAAAAAGAAATCGGACTCGGAGGACAATGGTCAGCTGCAGTTAGAAAAAAATGATCTCGCCAACGCTCCCATTAAGCGGAGCGAGGAGTCGGGTCAGTCCGTCCAACTCAAGGCGGACGATTTTGGTACTGCCCTTTCCAGTTCGGGATCAGGCGGCAACTCCAATCCCGGTTCCCCCACCCCCTGA", "aa": "MAQDPSNPYRFAVLLVPRSVVYYEQLQRGLGLPQQRTESGQNTSTTGAMFGLKVKNAEADTAKSNEKLQGAEATGSSTTSGSGQSTQRGGSSGDTKVKALKIEVKKKSDSEDNGQLQLEKNDLANAPIKRSEESGQSVQLKADDFGTALSSSGSGGNSNPGSPTP", "aa_hexdigest": "b75456ac175f9fc56726fb7e2d5d52d7", "start_type": "GTG", "rbs_motif": "AACAA", "psc": { "uniref90_id": "UniRef90_UPI001375E5B8", "query_cov": 0.806060606060606, "subject_cov": 0.875, "identity": 0.684, "score": 162.0, "evalue": 3.17e-48, "valid": false, "uniref50_id": "UniRef50_UPI001375E5B8" }, "pscc": { "uniref50_id": "UniRef50_UPI001375E5B8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001375E5B8" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17210.590299999974, "isoelectric_point": 7.880953788757323 }, "id": "APKECPOBEN_354", "locus": "GCA002090215_00318" }, { "type": "cds", "sequence": "contig_1", "start": 182105, "stop": 182449, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF12378.14" ], "nt": "TTGCTCCAAACCACCGGGTTCTTCAACCCGCGCCGCCACCCCGAGTGGTTTGATGGCGGGCAGACGGTCGCGGATAACGAAAAGACCGGGTTTGATGTGGATAACTCTGAAAACACCAAGCAGGGCTTTCAAAAGGAAGCTGACTCCGACAAGTCGGCCCCGATCGCCCTCCCGTTTGAAGCGTACTTCGCCAACATTGGCAACCTCACCTGGTTCGGGCAAGCGCTTTTGGTGTTTGGTGGCAATGGCCATGTTACCAAGTCGGCCCACACCGCGCCTTTGAGTATAGGTGTCTTTAGGGTGCGCTATAATGCAACTGGTACCAGTGCTACTGTAACTGGTTGA", "aa": "MLQTTGFFNPRRHPEWFDGGQTVADNEKTGFDVDNSENTKQGFQKEADSDKSAPIALPFEAYFANIGNLTWFGQALLVFGGNGHVTKSAHTAPLSIGVFRVRYNATGTSATVTG", "aa_hexdigest": "04e5edc98708378f800992127bd8d71b", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF12378.14", "name": "P1_N", "length": 111, "aa_cov": 0.9736842105263158, "hmm_cov": 0.7657657657657657, "evalue": 1.091604196773912e-32, "score": 109.00016021728516, "start": 4, "stop": 114 } ], "seq_stats": { "molecular_weight": 12343.444699999985, "isoelectric_point": 5.828349876403809 }, "id": "APKECPOBEN_355", "locus": "GCA002090215_00319" }, { "type": "cds", "sequence": "contig_1", "start": 182474, "stop": 182731, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF12378.14" ], "nt": "ATGGTCAACAAACAAACTGACGGGTTAAAGGATCTACCCTTTAACAATAACCGCTGGTTTGAATATGTACCACGGATGGCAGTTGCTGGCGCTAAGTTCGTTGGTAGGGAACTCGTTTTAGCGGGTACCATTACCATGGGTGATACCGCTACCGTACCTCGCTTACTGTACGATGAACTTGAAAGCAACCTGAACTTAGTAGCGCAAGGCCAAGGTCTTTTACGCGAAGACTTGCAACTCTTCACACCCTACGGATGA", "aa": "MVNKQTDGLKDLPFNNNRWFEYVPRMAVAGAKFVGRELVLAGTITMGDTATVPRLLYDELESNLNLVAQGQGLLREDLQLFTPYG", "aa_hexdigest": "35def7768350e2807fd02b13c0281780", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF12378.14", "name": "P1_N", "length": 31, "aa_cov": 0.35294117647058826, "hmm_cov": 1.0, "evalue": 5.415138503416689e-08, "score": 27.959794998168945, "start": 1, "stop": 30 } ], "seq_stats": { "molecular_weight": 9502.775499999994, "isoelectric_point": 4.88976764678955 }, "id": "APKECPOBEN_356", "locus": "GCA002090215_00320" }, { "type": "cds", "sequence": "contig_1", "start": 183029, "stop": 183172, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTGCTGCTATTCTCAATCCGGAATTGTTAGCAGCTCTTCCCGACAAGGTTAAATACGGTAAGGAAAACGAGTTTGCTGCTAACGAGTACGAGCGCTTTAACCAGAAGTTAACGGTAGCTCCTACCCAAGGAACAAACTGA", "aa": "MFAAILNPELLAALPDKVKYGKENEFAANEYERFNQKLTVAPTQGTN", "aa_hexdigest": "d59c1f3728201f00d30247ea40cfac70", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5256.895600000003, "isoelectric_point": 5.1057558059692365 }, "id": "APKECPOBEN_357", "locus": "GCA002090215_00321" }, { "type": "cds", "sequence": "contig_1", "start": 183140, "stop": 183493, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTCGAGGGTTCTTCGAGGCGAACCGCCTGTAATCATCGTCTGACTGCCCCCGAGGGTGGCTTGGACATTCGCTTTGAGGCCGACGCCGATGTTGGAAAACGTCGGGAGAAAGGAACGCGAACCACTTGTGTTCGTACTAATTCCGCTGGACGACCCCGCGCTGGTTTGAGGCGCGGTTATATCATCCACGCCCCGGTGGTTATTGCCATACCTGTACCCATTCCCAATCCAGGGCACATAATCCAACACCTGGTCGAGCACCGACCCCACAAGGTTGAACCCGGTGGAGAAACGGGAAAGCGTGGGGGAGAAGTGGGATCAGTTTGTTCCTTGGGTAGGAGCTACCGTTAA", "aa": "MVEGSSRRTACNHRLTAPEGGLDIRFEADADVGKRREKGTRTTCVRTNSAGRPRAGLRRGYIIHAPVVIAIPVPIPNPGHIIQHLVEHRPHKVEPGGETGKRGGEVGSVCSLGRSYR", "aa_hexdigest": "c2d291d8abbed16d3a353fb897c75661", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12663.298299999991, "isoelectric_point": 10.511392784118652 }, "id": "APKECPOBEN_358", "locus": "GCA002090215_00322" }, { "type": "cds", "sequence": "contig_1", "start": 183593, "stop": 183766, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGACCAGCAGGGACAATCAGGTACCTCCGCGGGGAATCCCGACTCGTTAAAGCAGGATAATATTAGTAAGAGTGGGGATAGTTTAACCACGCAGGACGGCAATGCGATCGATCAACAAGAGGCCACCAACTACACCAACCTCCCCCCCAACCTCACCCCCACCGCTGATTGA", "aa": "MDQQGQSGTSAGNPDSLKQDNISKSGDSLTTQDGNAIDQQEATNYTNLPPNLTPTAD", "aa_hexdigest": "e48410f7493c2d1e23bc2152ca21f9fd", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5952.140500000003, "isoelectric_point": 4.0500284194946286 }, "id": "APKECPOBEN_359", "locus": "GCA002090215_00323" }, { "type": "cds", "sequence": "contig_1", "start": 183848, "stop": 184126, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTGGTGAATCGAAGTGGGTCCGATTCCAACAAATTCCAAGCCACCGACCAAAAATGGTCCTACACCGACTTACATTCGGACCAAACCAAACTGAACCTCCCCGCTTACGGTGAGGTGAATGGGTTGTTGAATCCGGCGTTGGTGGAAACCTATTTTGGGAACACGCGAGCGGGTGGTTCGGGGTCCAACACGACCAGTTCACCCGGTATCGGTTTTAAAATTCCCGAACAAAATAATGATTCCAAAGCCACCCTGATCACCCCCGGGTTGGCTTGA", "aa": "MLVNRSGSDSNKFQATDQKWSYTDLHSDQTKLNLPAYGEVNGLLNPALVETYFGNTRAGGSGSNTTSSPGIGFKIPEQNNDSKATLITPGLA", "aa_hexdigest": "eb95bf68f965ac10ef327835dc3169b9", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9777.602299999991, "isoelectric_point": 5.608667182922363 }, "id": "APKECPOBEN_360", "locus": "GCA002090215_00324" }, { "type": "cds", "sequence": "contig_1", "start": 184169, "stop": 184309, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAGCTTCCAGCTCGGCGGGTGGCTGGTCACCTTCACGGACTTTGTCAAACCCCGCGCGGGTTACCTCGGTCTCCAGTTAACGGGCTTGGATGCAAGTGATGCGACGCAGCGCGCCCTCATTTGGGCCCCCCGGCCCTGA", "aa": "MSFQLGGWLVTFTDFVKPRAGYLGLQLTGLDASDATQRALIWAPRP", "aa_hexdigest": "131bdf59a053df27d9477418d1e8d4e4", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5066.789500000002, "isoelectric_point": 8.342096138000489 }, "id": "APKECPOBEN_361", "locus": "GCA002090215_00325" }, { "type": "cds", "sequence": "contig_1", "start": 184349, "stop": 185389, "strand": "+", "frame": 2, "gene": "mgpA", "product": "adhesin P1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0009E1D434", "UniRef:UniRef90_UPI0009E1D434" ], "nt": "GTGGAGAGTGTGTGGGATTTGAAGGGGGTGTGGGCGGATCAAGCTCAGTCCGACTCGCAAGGATCTACCACCACCGCAACAAGGAACGCCTTACCGGAGCACCCGAATGCTTTGGCCTTTCAGGTGAGTGTGGTGGAAGCGAGTGCTTACAAGCCAAACACGAGCTCCGGCCAAACCCAATCCACTAACAGTTCCCCCTACCTGCACTTGGTGAAGCCTAAGAAAGTTACCCAATCCGACAAGTTAGACGACGATCTTAAAAACCTGTTGGACCCCAACCAGGTTCGCACCAAGCTGCGCCAAAGCTTTGGTACAGACCATTCCACCCAGCCCCAGCCCCAATCGCTCAAAACAACGACACCGGTATTTGGGACGAGTAGTGGTAACCTCAGTAGTGTGCTTAGTGGTGGGGGTGCTGGAGGGGGTTCTTCAGGCTCAGGTCAATCTGGCGTGGATCTCTCCCCCGTTGAAAAAGTGAGTGGGTGGCTTGTGGGGCAGTTACCAAGCACGAGTGACGGAAACACCTCCTCCACCAACAACCTCGCGCCTAATACTAATACGGGGAATGATGTGGTGGGGGTTGGTCGACTTTCTGAAAGCAACGCCGCAAAGATGAATGACGATGTTGATGGTATTGTACGCACCCCACTCGCTGAACTGTTAGATGGGGAAGGACAAACAGCTGACACTGGTCCACAAAGCGTGAAGTTCAAGTCTCCTGACCAAATTGACTTCAACCGCTTGTTTACCCACCCAGTCACCGATCTGTTTGATCCGGTAACTATGTTGGTGTATGACCAGTACATACCGCTGTTTATTGATATCCCAGCAAGTGTGAACCCTAAAATGGTTCGTTTAAAGGTCTTGAGCTTTGACACCAACGAACAGAGCTTAGGTCTCCGCTTAGAGTTCTTTAAACCTGATCAAGATACCCAACCAAACAACAACGTTCAGGTCAATCCGAATAACGGTGACTTCTTACCACTGTTAACGGCCTCCAGTCAAGGTCCCCAAACCTTGTTTAGTCCGTTTAACCAGTGA", "aa": "MESVWDLKGVWADQAQSDSQGSTTTATRNALPEHPNALAFQVSVVEASAYKPNTSSGQTQSTNSSPYLHLVKPKKVTQSDKLDDDLKNLLDPNQVRTKLRQSFGTDHSTQPQPQSLKTTTPVFGTSSGNLSSVLSGGGAGGGSSGSGQSGVDLSPVEKVSGWLVGQLPSTSDGNTSSTNNLAPNTNTGNDVVGVGRLSESNAAKMNDDVDGIVRTPLAELLDGEGQTADTGPQSVKFKSPDQIDFNRLFTHPVTDLFDPVTMLVYDQYIPLFIDIPASVNPKMVRLKVLSFDTNEQSLGLRLEFFKPDQDTQPNNNVQVNPNNGDFLPLLTASSQGPQTLFSPFNQ", "aa_hexdigest": "b296c61e4f18cb79b9b387bfe54f7282", "start_type": "GTG", "rbs_motif": null, "genes": [ "mgpA" ], "truncated": "both", "pseudogene": { "start": 184049, "stop": 185668, "observations": { "mutation": [ 184124, 184307, 185387 ], "stop": [ 184124, 184307, 185387 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0009E1D434", "query_cov": 1.0, "subject_cov": 0.5805369127516778, "identity": 0.997, "score": 657.0, "evalue": 5.15e-234, "gene_start": 1, "gene_end": 346, "reference_start": 142, "reference_end": 487, "reference_sequence": "SDQTKLNLPAYGEVNGLLNPALVETYFGNTRAGGSGSNTTSSPGIGFKIPEQNNDSKATLITPGLAWTPQDVGNLVVSGTTVSFQLGGWLVTFTDFVKPRAGYLGLQLSGLNASDSDQRELIWAPRPWAAFRGSWVNRLGRVESVWDLKGVWADQAQSDSQGSTTTATRNALPEHPNALAFQVSVVEASAYKPNTSSGQTQSTNSSPYLHLVKPKKVTQSDKLDDDLKNLLDPNQVRTKLRQSFGTDHSTQPQPQSLKTTTPVFGTSSGNLSSVLSGGGAGGGSSGSGQSGVDLSPVEKVSGWLVGQLPSTSDGNTSSTNNLAPNTNTGNDVVGVGRLSESNAAKMNDDVDGIVRTPLAELLDGEGQTADTGPQSVKFKSPDQIDFNRLFTHPVTDLFDPVTMLVYDQYIPLFIDIPASVNPKMVRLKVLSFDTNEQSLGLRLEFFKPDQDTQPNNNVQVNPNNGDFLPLLTASSQGPQTLFSPFNQWPDYVLPLAITVPIVVIVLSVTLGLAIGIPMHKNKQALKAGFALSNQKVDVLTKAVGSVFKEIINRTGISQAPKRLKQTSAAKPGAPRPPVPPKPGAPKPPVQPPKKPA" }, "paralog": false, "identity": 0.9851576994434137, "score": 1042.0, "evalue": 0.0, "description": "Internal stop codon at 184124, 184307, 185387. Nonsense mutation around 184124, 184307, 185387.", "psc": { "uniref90_id": "UniRef90_UPI0009E1D434", "gene": "mgpA", "product": "adhesin P1", "uniref50_id": "UniRef50_UPI0009E1D434", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0009E1D434", "UniRef:UniRef50_UPI0009E1D434" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0009E1D434", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0009E1D434" ], "product": "adhesin P1" } }, "id": "APKECPOBEN_362", "locus": "GCA002090215_00326" }, { "type": "cds", "sequence": "contig_1", "start": 185399, "stop": 185716, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTGCCGTTAGCGATCACTGTACCTATTGTTGTGATTGTGCTCAGTGTTACCTTAGGACTTGCCATTGGAATCCCAATGCACAAGAACAAACAGGCCTTGAAGGCTGGGTTTGCGCTATCAAACCAAAAGGTTGATGTGTTGACCAAAGCGGTTGGTAGTGTCTTTAAGGAAATCATTAACCGCACAGGTATCAGTCAAGCGCCAAAACGCTTGAAACAAACCAGTGCGGCTAAACCAGGAGCACCCCGCCCACCAGTACCACCAAAGCCAGGGGCTCCTAAGCCACCAGTGCAACCACCTAAAAAACCCGCTTAG", "aa": "MLPLAITVPIVVIVLSVTLGLAIGIPMHKNKQALKAGFALSNQKVDVLTKAVGSVFKEIINRTGISQAPKRLKQTSAAKPGAPRPPVPPKPGAPKPPVQPPKKPA", "aa_hexdigest": "73882c2df7ebb5c3c334b6e0860da49d", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10932.12489999998, "isoelectric_point": 11.35315647125244 }, "id": "APKECPOBEN_363", "locus": "GCA002090215_00327" }, { "type": "cds", "sequence": "contig_1", "start": 185722, "stop": 185922, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATCGAAGCTAAAGTTAAAACGTTATTTACTGTTTTTACCACTTTTACCGCTAGGGACGTTGTCACTAGCCAACACCTACCTCCTCCAAGACCACAACACCCTCACCCCCTACACGCCCTTTACGACACCGCTCAATGGGGGGCTGGATGTCGTGCGCGCCGCCCATTTACACCCCTCATACGAACTCGTGGACTGA", "aa": "MKSKLKLKRYLLFLPLLPLGTLSLANTYLLQDHNTLTPYTPFTTPLNGGLDVVRAAHLHPSYELVD", "aa_hexdigest": "305064b624de3835b370f99498e1956b", "start_type": "ATG", "rbs_motif": "AAAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7434.653600000002, "isoelectric_point": 9.157943534851075 }, "id": "APKECPOBEN_364", "locus": "GCA002090215_00328" }, { "type": "cds", "sequence": "contig_1", "start": 185929, "stop": 186495, "strand": "+", "frame": 1, "gene": null, "product": "Putative MgpC-like protein MPN_149", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75037", "UniRef:UniRef90_P75037" ], "nt": "GTGGGGGATACCAAGTTGGTGGCGCTGGTCCGCTCAGCGTTGGTCAGGGTGAAATTCCAGGACACAACGAGTTCGGATCAAAGTAATACCAACCAAAATGCCTTGAGTTTTGATACCCAAGAATCACAGAAGGCACTTAATGGCTCGCAGAGTGGATCTTCTGACACTTCCGGGTCTAACTCCCAAGACTTCGCCAGCTATGTCCTCATCTTTAAAGCCGCGCCCAGGGCCACGTGGGTGTTTGAACGCAAGATTAAGTTGGCGTTGCCCTACGTTAAGCAGGAAAGTCAGGGTTCCGGCGATCAAGGTTCCAATGGTAAGGGCTCCCTCTACAAAACCCTCCAAGACCTCCTCGTCGAACAACCCGTGACCCCTTACACCCCGAATGCGGGGTTAGCCCGGGTGAATGGGGTTGCTCAGGATACGGTTCATTTTGGTTCGGGTCAAGAATCGAGTTGGAATTCCCAACGTTCCCAAAAAGGCCTTAAAAACAACCCCGGACCCAAAGCCGTCACCGGCTTTAAGCTCGATAAGGGCCGCGCGTACCGGAAGCTGAATGAAAGTTGA", "aa": "MGDTKLVALVRSALVRVKFQDTTSSDQSNTNQNALSFDTQESQKALNGSQSGSSDTSGSNSQDFASYVLIFKAAPRATWVFERKIKLALPYVKQESQGSGDQGSNGKGSLYKTLQDLLVEQPVTPYTPNAGLARVNGVAQDTVHFGSGQESSWNSQRSQKGLKNNPGPKAVTGFKLDKGRAYRKLNES", "aa_hexdigest": "4ad845034cc880b4c4a544d317148f1a", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 185893, "stop": 186726, "observations": { "mutation": [ 185920, 186493 ], "stop": [ 185920, 186493 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75037", "query_cov": 1.0106382978723405, "subject_cov": 0.4377880184331797, "identity": 0.879, "score": 310.0, "evalue": 3.16e-102, "gene_start": 1, "gene_end": 188, "reference_start": 22, "reference_end": 210, "reference_sequence": "MAVGIFILSLNPSYELVDWKRVGDTKLVALVRSALVRVKFNDGTSSDSNNQDTNQNALSFDTQESQKALNGSQSGSSDTSGSNSQDFASYILIFQAAPRATWVFERKIKLELPYVKNESGAGDSTTTNSGSLYTTLQDLLVEQPVTPYTPNAGLARVNGAAQDTVHFGSGQESSWNSQRSQKVLKNNPGPKAVTGFKLDKGRAYRKLNEAWPVYEPLDSTKDGKGKDESSWNSSEKTTAESDAPLVGSTGSQMAAVTDSQQSGDNNGLVSLAQRSTTVAVQKSDSSGSQGQGTTDNKFQKYLNTTQALHQMGVIVPSLETWAGENKYWNRYPCCWWCFSPSSDPAIVFHKWRSTECNHPVIPYFNRPTRLLKWPDRCDGFERGTEFVVLGRGWANHVRKGYLMSPHRVELGHRQAEGVCGESAWFQWNQRHRLA" }, "paralog": false, "identity": 0.8071428571428572, "score": 422.0, "evalue": 1.05e-148, "description": "Internal stop codon at 185920, 186493. Nonsense mutation around 185920, 186493.", "psc": { "uniref90_id": "UniRef90_P75037", "product": "Putative MgpC-like protein MPN_149", "uniref50_id": "UniRef50_P75037", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75037", "UniRef:UniRef50_P75037" ] }, "pscc": { "uniref50_id": "UniRef50_P75037", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75037" ], "product": "Putative MgpC-like protein MPN_149" } }, "id": "APKECPOBEN_365", "locus": "GCA002090215_00329" }, { "type": "cds", "sequence": "contig_1", "start": 186499, "stop": 186915, "strand": "+", "frame": 1, "gene": "mgpC", "product": "MgpC family cytadherence protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75414", "UniRef:UniRef90_UPI001F33AF27" ], "nt": "GTGTATGAACCCCTGGATTCGACCAAGGAGGGGAAGGGGAAGGATGAGAGCTCTTGGAAAAATTCGGAAAAAACAACAGCGGAAAATGATGCCCCGTTGGTGGGGATGGTTGGAAGTGGTGCGGCTGGAAGTGCTTCTAGTTTACAAGGCAATGGCTCGAACAGTTCGGGGTTAAAATCGCTCTTGAGATCAGCACCTGTCAGTGTTCCACCAAGCAGTACAAGTAATCAAACTTTAAGCTTATCTAACCCCGCTCCTGTGGGCCCACAAGCGGTTGTAAGCCAACCCGCGGGGGGTGCTACGGCAGCAGTGTCCGTCAATCGCACAGCGAGTGACACCGCCACCTTTAGCAAGTACCTCAACACCGCCCAGGCCTTGCACCAGATGGGGGTGATTGTTCCGGGGTTGGAAAAATGA", "aa": "MYEPLDSTKEGKGKDESSWKNSEKTTAENDAPLVGMVGSGAAGSASSLQGNGSNSSGLKSLLRSAPVSVPPSSTSNQTLSLSNPAPVGPQAVVSQPAGGATAAVSVNRTASDTATFSKYLNTAQALHQMGVIVPGLEK", "aa_hexdigest": "8843f90dd8ff213ac750e6c3d8273e11", "start_type": "GTG", "rbs_motif": null, "genes": [ "mgpC" ], "truncated": "3-prime", "pseudogene": { "start": 186499, "stop": 187218, "observations": { "mutation": [ 186913 ], "stop": [ 186913 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001F33AF27", "query_cov": 1.0, "subject_cov": 0.4726027397260274, "identity": 0.993, "score": 246.0, "evalue": 7.28e-80, "gene_start": 1, "gene_end": 138, "reference_start": 1, "reference_end": 138, "reference_sequence": "MYEPLDSTKEGKGKDESSWKNSEKTTAENDAPLVGMVGSGAAGSASSLQGNGSNSSGLKSLLRSAPVSVPPSSTSNQTLSLSNPAPVGPQAVVSQPAGGATAAVSVNRTASDTSTFSKYLNTAQALHQMGVIVPGLEKWGGNNGTGVVASRRDATSTNLPHAAGASQTGLGTGSPREPALTATSQRAVTVVAGPLRAGNSSETDALPNVITQLYHTSTAQLAYLNGQIVVMGSDRVPSLWYWVVGEDQESGKATWWAKTELNWGTDKQKQFVENQLGFKDDSNSDSKNSNLK" }, "paralog": false, "identity": 0.9832635983263598, "score": 456.0, "evalue": 4.8e-165, "description": "Internal stop codon at 186913. Nonsense mutation around 186913.", "psc": { "uniref90_id": "UniRef90_UPI001F33AF27", "product": "MgpC family cytadherence protein", "uniref50_id": "UniRef50_P75414", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001F33AF27", "UniRef:UniRef50_P75414" ] }, "pscc": { "uniref50_id": "UniRef50_P75414", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75414" ], "product": "Putative MgpC-like protein MPN_367" } }, "id": "APKECPOBEN_366", "locus": "GCA002090215_00330" }, { "type": "cds", "sequence": "contig_1", "start": 186940, "stop": 187224, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGCTAGCCGACAGGATGCTACTTCCACTAACCTGCCCCATGCGGCAGGTGCTTCCCAAACGGGTTTGGGAACTGGTTCGCCCCGCGAACCAGCTTTAACCGCAACGTCACAGCGTGCCGTCACGGTGGTTGCTGGCCCCCTTCGTGCGGGCAATAGCAGTGAAACTGATGCCCTACCGAATGTCATCACCCAGCTCTATCATACTTCAACCGCCCAACTCGCTTACTTAAATGGCCAGATCGTTGTGATGGGTTCCGACCGGGTACCGAGTCTTTGGTATTGA", "aa": "MASRQDATSTNLPHAAGASQTGLGTGSPREPALTATSQRAVTVVAGPLRAGNSSETDALPNVITQLYHTSTAQLAYLNGQIVVMGSDRVPSLWY", "aa_hexdigest": "baccf4791daf85b869714132ce5c9fab", "start_type": "GTG", "rbs_motif": "AACAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9724.7005, "isoelectric_point": 6.702817726135254 }, "id": "APKECPOBEN_367", "locus": "GCA002090215_00331" }, { "type": "cds", "sequence": "contig_1", "start": 187324, "stop": 187962, "strand": "+", "frame": 1, "gene": "mgpC", "product": "MgpC family cytadherence protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002246AD21", "UniRef:UniRef90_UPI002246AD21" ], "nt": "TTGGGGTTTAAAGATGACTCAAATTCGGATTCCAAAAATTCGAATTTGAAGGCCCAAGGCCTCACCCAACCCGCCTACCTCATCGCCGGTCTTGACGTTGTGGCCGACCACCTCGTCTTTGCGGCCTTTAAAGCGGGCGCGGTGGGGTATGATATGACGACTGATTCGAGCGCTTCGACCTACAACCAAGCACTCGCCTGGTCGACCACGGCCGGGTTGGACAGTGATGGGGGGTACAAGGCCTTGGTGGAAAACACGGCCGGGCTCAACGGCCCGATTAATGGCTTGTTTACCCTGCTCGACACCTTTGCGTATGTGACCCCCGTGAGTGGGATGAAAGGGGGGAGTCAGAATAATGAAGAAGTGCAAACGACTTACCCGGTCAAGTCCGACCAAAAGGCCACCGCCAAAATTGCCTCCTTAATTAATGCCAGCCCACTCAACAGTTATGGGGATGATGGGGTGACCGTGTTTGATGCCCTGGGCCTTAACTTTAACTTTAAGTTGAACGAGGAGCGCTTGCCATCGCGCACCGACCAACTGCTTGTGTATGGGATTGTAAACGAAAGTGAACTGAAGTCCGCACGGGAAAATGCCCAGTCGACCTCCGATGATAATTCAAACACCAAAGTCAAGTGA", "aa": "MGFKDDSNSDSKNSNLKAQGLTQPAYLIAGLDVVADHLVFAAFKAGAVGYDMTTDSSASTYNQALAWSTTAGLDSDGGYKALVENTAGLNGPINGLFTLLDTFAYVTPVSGMKGGSQNNEEVQTTYPVKSDQKATAKIASLINASPLNSYGDDGVTVFDALGLNFNFKLNEERLPSRTDQLLVYGIVNESELKSARENAQSTSDDNSNTKVK", "aa_hexdigest": "02a92d6dfe7deb41122e1d09289fc3d7", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002246AD21", "query_cov": 0.9292452830188679, "subject_cov": 0.8565217391304348, "identity": 0.914, "score": 340.0, "evalue": 9.84e-117, "valid": true, "product": "MgpC family cytadherence protein", "uniref50_id": "UniRef50_UPI002246AD21" }, "pscc": { "uniref50_id": "UniRef50_UPI002246AD21", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002246AD21" ], "product": "MgpC family cytadherence protein" }, "genes": [ "mgpC" ], "id": "APKECPOBEN_368", "locus": "GCA002090215_00332" }, { "type": "cds", "sequence": "contig_1", "start": 187990, "stop": 189378, "strand": "+", "frame": 1, "gene": "mgpC", "product": "MgpC family cytadherence protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000AFF1225", "UniRef:UniRef90_UPI000AFF1225" ], "nt": "GTGCCGTATTACTACAGTGCCAATTTCCCCGAAGCGGGTAACAGAAGGCGAGCGGAGCAGCGGAATGGGGTGAAGATTAGCACCTTGGAATCGCAAGCCACTGATGGCTTTGCCAACTCGTTACTTAACTTTGGTACCGGTCTTAAAGCCGGTGTTGACCCAGCTCCAGTAGCACGGGGTCATAAACCGAACTATAGTGCAGTACTACTAGTGCGTGGTGGCGTTGTAAGGTTAAACTTTAACCCCGATACTGATAAACTGTTGGATTCTACTGACAAAAACAGTGAACCTATCTCCTTCTCCTATACCCCATTTGGGTCTGCTGAAAGTGCCGTAGACCTCACCACGTTGAAGGATGTGACCTATATTGCTGAAAGTGGTCTGTGGTTCTATACCTTTGACAATGGTGAAAAACCAACGTACGATGGTAAACAACAACAGGTCAAAAACCGCAAGGGTTATGCTGTGATTACCGTATCACGTACCGGAATTGAATTTAACGAGGACGCTAATACCACAACCTTAAGCCAAGCCCCAGCTGCTTTGGCTGTCCAAAACGGGATTGCTTCCAGTCAGGACGACCTCACAGGCATCCTACCGTTATCCGATGAGTTCTCCGCTGTGATTACCAAGGATCAAACATGGACCGGTAAGGTTGATATCTATAAGAACACCAACGGGTTGTTTGAAAAGGATGATCAGCTATCGGAAAACGTGAAGAGGCGTGACAACGGTTTGGTCCCTATTTACAACGAAGGTATCGTCGATATTTGGGGCAGAGTGGATTTTGCTGCCAACAGTGTTTTGCAAGCGCGTAACCTCACTGATAAAACGGTTGATGAGGTGATCAATAACCCCGATATCCTCCAAAGCTTCTTTAAGTTTACCCCAGCCTTTGATAACCAAAGAGCAATGCTAGTGGGGGAAAAGACATCGGATACTACCTTAACGGTTAAACCGAAGATTGAGTACTTGGATGGTAACTTCTATGGTGAGGATTCCAAGATTGCTGGAATTCCGCTCAACATTGATTTCCCTTCCCGGATTTTTGCTGGCTTTGCTGCTTTACCGTCCTGGGTCATTCCGGTATCAGTCGGTTCATCGGTGGGCATTCTCTTAATCCTGCTCATCTTAGGCCTTGGTATTGGAATTCCAATGTATAAGGTCCGCAAGCTTCAAGACTCCAGCTTTGTTGATGTGTTTAAAAAGGTGGATACGTTGACAACCGCTGTGGGTAGCGTGTACAAGAAGATTATCACCCAAACGAGTGTGATCAAAAAAGCTCCTAGTGCGTTGAAAGCTGCTAATAACGCTGCTCCTAAAGCACCAGTTAAACCAGCTGCTCCAACAGCTCCAAGACCACCAGTCCAACCACCTAAAAAGGCTTAA", "aa": "MPYYYSANFPEAGNRRRAEQRNGVKISTLESQATDGFANSLLNFGTGLKAGVDPAPVARGHKPNYSAVLLVRGGVVRLNFNPDTDKLLDSTDKNSEPISFSYTPFGSAESAVDLTTLKDVTYIAESGLWFYTFDNGEKPTYDGKQQQVKNRKGYAVITVSRTGIEFNEDANTTTLSQAPAALAVQNGIASSQDDLTGILPLSDEFSAVITKDQTWTGKVDIYKNTNGLFEKDDQLSENVKRRDNGLVPIYNEGIVDIWGRVDFAANSVLQARNLTDKTVDEVINNPDILQSFFKFTPAFDNQRAMLVGEKTSDTTLTVKPKIEYLDGNFYGEDSKIAGIPLNIDFPSRIFAGFAALPSWVIPVSVGSSVGILLILLILGLGIGIPMYKVRKLQDSSFVDVFKKVDTLTTAVGSVYKKIITQTSVIKKAPSALKAANNAAPKAPVKPAAPTAPRPPVQPPKKA", "aa_hexdigest": "ee794a8dfd26ecf0921297c89b312275", "start_type": "GTG", "rbs_motif": null, "genes": [ "mgpC" ], "truncated": "5-prime", "pseudogene": { "start": 187690, "stop": 189378, "observations": { "mutation": [ 187960 ], "stop": [ 187960 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000AFF1225", "query_cov": 1.0, "subject_cov": 0.6666666666666666, "identity": 0.998, "score": 855.0, "evalue": 8.33e-309, "gene_start": 1, "gene_end": 462, "reference_start": 232, "reference_end": 693, "reference_sequence": "AFKAGAVGYDMTTDSSASTYNQALAWSTTAGLDSDGGYKALVENTAGLNGPINGLFTLLDTFAYVTPVSGMKGGSQNNEEVQTTYPVKSDQKATAKILNGPINDLFTLLDTFAYVTPVSGMKGGSQNNEEVQTKYPVKDDSKASAKIASLINASPLNSYGDDGVTVFDALGLNFNFKLNEERLPSRTDQLLVYGIVNESELKSARENAQSTSDDNSNTKVKWTNTASHYLPVPYYYSANFPEAGNRRRAEQRNGVKISTLESQATDGFANSLLNFGTGLKAGVDPAPVARGHKPNYSAVLLVRGGVVRLNFNPDTDKLLDSTDKNSEPISFSYTPFGSAESAVDLTTLKDVTYIAESGLWFYTFDNGEKPTYDGKQQQVKNRKGYAVITVSRTGIEFNEDANTTTLSQAPAALAVQNGIASSQDDLTGILPLSDEFSAVITKDQTWTGKVDIYKNTNGLFEKDDQLSENVKRRDNGLVPIYNEGIVDIWGRVDFAANSVLQARNLTDKTVDEVINNPDILQSFFKFTPAFDNQRAMLVGEKTSDTTLTVKPKIEYLDGNFYGEDSKIAGIPLNIDFPSRIFAGFAALPSWVIPVSVGSSVGILLILLILGLGIGIPMYKVRKLQDSSFVDVFKKVDTLTTAVGSVYKKIITQTSVIKKAPSALKAANNAAPKAPVKPAAPTAPRPPVQPPKKA" }, "paralog": false, "identity": 0.9166666666666666, "score": 1037.0, "evalue": 0.0, "description": "Internal stop codon at 187960. Nonsense mutation around 187960.", "psc": { "uniref90_id": "UniRef90_UPI000AFF1225", "product": "MgpC family cytadherence protein", "uniref50_id": "UniRef50_UPI000AFF1225", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000AFF1225", "UniRef:UniRef50_UPI000AFF1225" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000AFF1225", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000AFF1225" ], "product": "MgpC family cytadherence protein" } }, "id": "APKECPOBEN_369", "locus": "GCA002090215_00333" }, { "type": "cds", "sequence": "contig_1", "start": 189495, "stop": 189776, "strand": "+", "frame": 3, "gene": null, "product": "putative protein MPN_143", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75143", "UniRef:UniRef90_P75143" ], "nt": "ATGAGTCATAAAGATTTTAATGGGCTTCAAGCCCCTCAGTTGTTAAGTTCGAGTTCCCCTGTTGCAAAGAAACAAAGTAGTCATAAACTCAGGCATGCCTTAAAGCACGCCCGTTACTTAAACCATAGTAGTAAGCGTACCCTCAAGCATGCCTTAGAGCTGCATGAAGACAACCAGGTCTTACTGGAAAAAGAAGGTAGTCCTAACTTCCAGGACTGGCTTTCTAAGCAACCTGGGGTCAATAAAACCAGCCTTAAGTACAACAAAAGCTTGGGTAGTTGA", "aa": "MSHKDFNGLQAPQLLSSSSPVAKKQSSHKLRHALKHARYLNHSSKRTLKHALELHEDNQVLLEKEGSPNFQDWLSKQPGVNKTSLKYNKSLGS", "aa_hexdigest": "6fb5f06c63730a34196b2433ee85fb01", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 189495, "stop": 190022, "observations": { "mutation": [ 189774 ], "stop": [ 189774 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75143", "query_cov": 1.0, "subject_cov": 0.5314285714285715, "identity": 1.0, "score": 179.0, "evalue": 3.88e-56, "gene_start": 1, "gene_end": 93, "reference_start": 1, "reference_end": 93, "reference_sequence": "MSHKDFNGLQAPQLLSSSSPVAKKQSSHKLRHALKHARYLNHSSKRTLKHALELHEDNQVLLEKEGSPNFQDWLSKQPGVNKTSLKYNKSLGSWISKESKPKKRFPPYFTYKGSKTTPEEAKALQQMKQSQKRFFHENMHSFLNEVAHNPMIQRFKQKQAKRAANTRQRTYKYRQ" }, "paralog": false, "identity": 0.9942857142857143, "score": 353.0, "evalue": 5.66e-127, "description": "Internal stop codon at 189774. Nonsense mutation around 189774.", "psc": { "uniref90_id": "UniRef90_P75143", "product": "Uncharacterized protein MPN_143", "uniref50_id": "UniRef50_P75143", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75143", "UniRef:UniRef50_P75143" ] }, "pscc": { "uniref50_id": "UniRef50_P75143", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75143" ], "product": "Uncharacterized protein MPN_143" } }, "id": "APKECPOBEN_370", "locus": "GCA002090215_00334" }, { "type": "cds", "sequence": "contig_1", "start": 189873, "stop": 190022, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAACAATCCCAAAAGCGCTTCTTTCACGAAAATATGCACAGCTTTCTCAATGAAGTTGCACATAACCCAATGATCCAGCGCTTTAAGCAAAAGCAGGCCAAACGGGCTGCTAATACGCGACAACGCACTTACAAATACCGTCAGTAA", "aa": "MKQSQKRFFHENMHSFLNEVAHNPMIQRFKQKQAKRAANTRQRTYKYRQ", "aa_hexdigest": "39f1a2e8718261f6ccbf2cebf4ce3892", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6138.989000000003, "isoelectric_point": 11.434837913513185 }, "id": "APKECPOBEN_371", "locus": "GCA002090215_00335" }, { "type": "cds", "sequence": "contig_1", "start": 190025, "stop": 190156, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W9U9", "UniRef:UniRef90_A0AB38W9U9" ], "nt": "ATGCACAAGATTAAACACTATAAACCCTTAAAGCGCAAGACCTGGTTAAACAAGGTTTCCAAGCAACGCCGCGAGCGCATGTCCGCTAAGTGCTATCAACCACCTAAGAGAGAAGTGATGCAGCCAACTTGA", "aa": "MHKIKHYKPLKRKTWLNKVSKQRRERMSAKCYQPPKREVMQPT", "aa_hexdigest": "7feeb616217eaab4132de434a7c43250", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0AB38W9U9", "query_cov": 1.0, "subject_cov": 0.9347826086956522, "identity": 1.0, "score": 86.7, "evalue": 8.35e-22, "valid": true, "uniref50_id": "UniRef50_A0AB38W9U9" }, "pscc": { "uniref50_id": "UniRef50_A0AB38W9U9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W9U9" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5366.414400000002, "isoelectric_point": 10.8630033493042 }, "id": "APKECPOBEN_372", "locus": "GCA002090215_00336" }, { "type": "cds", "sequence": "contig_1", "start": 190128, "stop": 190496, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTCGAGGGTTCTTCAAGGCGAACCGCCCACAGTCGTCGTCTGACTGTCCCCGAGGGTGGCTTGGACATTCGCTTTGAGGCCAACGCCGATGTTGGAAAACGTCGGGAAAAAGGAACGCGAACCACTTGTGTTCGTACTAGTTCCGCTGGACGACCCCGCGCTGGTTTGAGGCGCGGTTATATCATTCACGCCCCGGTGGTTATTCCCATACCTGTACCCATTCCCAATCCAGGGAATATAATCCAACACCTGGTCCAGAACAGACCCCACAAGGTTGAACCCGGTGGAGAAACGGGAAAGCGTGGGGGAGAAGTGGGATCAGGTGGTGCTAACTCTTTCAAGTTGGCTGCATCACTTCTCTCTTAG", "aa": "MVEGSSRRTAHSRRLTVPEGGLDIRFEANADVGKRREKGTRTTCVRTSSAGRPRAGLRRGYIIHAPVVIPIPVPIPNPGNIIQHLVQNRPHKVEPGGETGKRGGEVGSGGANSFKLAASLLS", "aa_hexdigest": "a1a61358d26e50b508301bef793f3d91", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12963.645599999985, "isoelectric_point": 11.435998344421389 }, "id": "APKECPOBEN_373", "locus": "GCA002090215_00337" }, { "type": "cds", "sequence": "contig_1", "start": 190596, "stop": 190769, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGCCAGCAGGGACAATCAGGTACCTCCGCGGGGAATCCAGACTCGTTAAAGCAGGATAAGATTAGTAAGAGTGGGGATAGTTTAACCACGCAGGACGGCAATGCGACCGGTCAACAAGAGGCCACTAACTACACCAACCTCCCCCCCAACCTCACCCCCACCGCTGATTGA", "aa": "MGQQGQSGTSAGNPDSLKQDKISKSGDSLTTQDGNATGQQEATNYTNLPPNLTPTAD", "aa_hexdigest": "05af81cce8b055a65f0b9ea419c5cb62", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5838.084300000002, "isoelectric_point": 4.306201744079591 }, "id": "APKECPOBEN_374", "locus": "GCA002090215_00338" }, { "type": "cds", "sequence": "contig_1", "start": 190851, "stop": 191129, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTGGTGAATCGAAGTGGGTCCGATTCCAACAAATTCCAAGCCACCGACCAAAAATGGTCCTACACCGACTTACAGTCGGACCAAACCAAACTGAACCTCCCCGCTTACGGTGAGGTGAATGGGTTGTTGAATCCGGCGTTGGTGGAAACCTATTTTGGGAACACGCGAGCGGGTGGTTCGGGGTCCAACACGACCAGTTCACCCGGTATCGGTTTTAAAATTCCCGAACAAAATAATGATTCCAAAGCCACCCTGATCACCCCCGGGTTGGCTTGA", "aa": "MLVNRSGSDSNKFQATDQKWSYTDLQSDQTKLNLPAYGEVNGLLNPALVETYFGNTRAGGSGSNTTSSPGIGFKIPEQNNDSKATLITPGLA", "aa_hexdigest": "39714ed164769acd21b8843301f92ef5", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9768.59219999999, "isoelectric_point": 5.086487388610839 }, "id": "APKECPOBEN_375", "locus": "GCA002090215_00339" }, { "type": "cds", "sequence": "contig_1", "start": 191352, "stop": 191837, "strand": "+", "frame": 3, "gene": null, "product": "adhesin", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75593", "UniRef:UniRef90_UPI001330EAF6" ], "nt": "GTGGAGAGTGTGTGGGATTTAAAGGGGGTGTGGGCGGATCAAGCTCAGTTGGCCGCGCAAGCAGCTACAAGTGAAGCTTCCGGGTCAGCTTTGGCACCTCACCCGAATGCTTTGGCGTTTCAGGTGAGTGTGGTGGAAGCGAGTGCTTACAGCTCTTCAACCTCAAGTTCGGGTTCCGGGTCAAGTTCAAACACCTCCCCCTACCTCCATTTGATTAAACCTAAGAAAGTCGAAAGCACGACCCAACTCGACCAGGGCTTAAAAAACCTGTTGGACCCCAACCAGGTTCGCACCAAGCTGCGCCAAAGCTTTGGTACAGACCATTCCACCCAGCCCCAATCGCTCAAAACAACGACACCGGTATTTGGGACGAGTAGTGGTAACATTGGCAGTGTGCTTAGTGGTGGGGGTGCTGGAGGGGGTTCTTCAGGCTCAGGTCAATCTGGTGTGGACCTCTCCCCCGTTGAACGGGTGAGTGGTCACTAA", "aa": "MESVWDLKGVWADQAQLAAQAATSEASGSALAPHPNALAFQVSVVEASAYSSSTSSSGSGSSSNTSPYLHLIKPKKVESTTQLDQGLKNLLDPNQVRTKLRQSFGTDHSTQPQSLKTTTPVFGTSSGNIGSVLSGGGAGGGSSGSGQSGVDLSPVERVSGH", "aa_hexdigest": "a8c1766577b833290fb86c4d69c004ea", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 191052, "stop": 191837, "observations": { "mutation": [ 191127, 191310 ], "stop": [ 191127, 191310 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001330EAF6", "query_cov": 1.0124223602484472, "subject_cov": 0.4515235457063712, "identity": 0.975, "score": 239.0, "evalue": 5.61e-76, "gene_start": 1, "gene_end": 161, "reference_start": 199, "reference_end": 361, "reference_sequence": "ADWPNALSFTNKNNAQRAQLFLRGLLGSIPVLVNKSGQDDNSKFKAEDQKWSYTDLQSDQTKLNLPAYGEVNGLLNPALVETYFGNTRASGSGSNTTSSPGIGFKIPEQNNDSKATLITPGLAWTPQDVGNLVVSGTSLSFQLGGWLVSFTDFIKPRAGYLGLQLSGLDASDSDQRELIWAKRPWAAFRGSWVNRLGRVESVWDLKGVWADQAQLAAQAATSEASGSALAPHPNALAFQVSVVEASAYSSSTSSSGSGSSSNTSPYLHLIKPKKVESTTQLDQGLKNLLDPNQVRTKLRQSFGTDHSTQPQPQSLKTTTPVFGTSSGNIGSVLSGGGAGGGSSGSDQSGVDLSPVERVSGH" }, "paralog": false, "identity": 0.9809885931558935, "score": 443.0, "evalue": 1.99e-158, "description": "Internal stop codon at 191127, 191310. Nonsense mutation around 191127, 191310.", "psc": { "uniref90_id": "UniRef90_UPI001330EAF6", "product": "adhesin", "uniref50_id": "UniRef50_P75593", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001330EAF6", "UniRef:UniRef50_P75593" ] }, "pscc": { "uniref50_id": "UniRef50_P75593", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75593" ], "product": "Putative adhesin P1-like protein MPN_099" } }, "id": "APKECPOBEN_376", "locus": "GCA002090215_00340" }, { "type": "cds", "sequence": "contig_1", "start": 192189, "stop": 192728, "strand": "+", "frame": 3, "gene": null, "product": "UPF0134 protein MPN_145", "db_xrefs": [ "RefSeq:WP_010874502.1", "SO:0001217", "UniParc:UPI000013A21F", "UniRef:UniRef100_P75141", "UniRef:UniRef50_P75141", "UniRef:UniRef90_P75141" ], "nt": "ATGTCTTACTCACCTTCACTTAAGGAAATTATTGCTATTTTGCAAAAATACACTTCGAAAAACTACCAGTCAAATTGCAAAACTAGACCCGATGGTAAGCTTGAATTATCATTAAATGGAGTTTTTGAAGAAATCGTGAAAACACCCGGTAAAACTAGGTACGTAACCCATAAACAGCTTGATCAGAAATTAAAAGATTTCAAGCAAGATCTGATGGTTGAGTTGCACGACACGTTTGCTACTAAAACTGATCTTAAAGAATCGGAAGCACGCATTAACCAAAAGTTAGAAGCACTAATTCAAATTGTTATGGTTCAGGGTGAGCAGATAAAAGTTCACGGCGAGCAAATTAACAAACTGACTCAAGCTGTTGAAAAACAGGGCGAGAAAATTGAAGCTCAAGGACAACAAATTCAAAAAGTTAATGAAACTTTGAATTTTGTAGTTGAAAGCCTTGGATCTATCCACAAAAGACTCGATTCAATGGAAGGCCGCTTGGACTCAATGGAAAACCGTCTTGACAAACTAGAGTCCAAGTAG", "aa": "MSYSPSLKEIIAILQKYTSKNYQSNCKTRPDGKLELSLNGVFEEIVKTPGKTRYVTHKQLDQKLKDFKQDLMVELHDTFATKTDLKESEARINQKLEALIQIVMVQGEQIKVHGEQINKLTQAVEKQGEKIEAQGQQIQKVNETLNFVVESLGSIHKRLDSMEGRLDSMENRLDKLESK", "aa_hexdigest": "5a6b270501c5cc4aee486920fb5bfdca", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013A21F", "ncbi_nrp_id": "WP_010874502.1", "uniref100_id": "UniRef100_P75141", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013A21F", "RefSeq:WP_010874502.1", "UniRef:UniRef100_P75141" ] }, "ips": { "uniref100_id": "UniRef100_P75141", "uniref90_id": "UniRef90_P75141" }, "psc": { "uniref90_id": "UniRef90_P75141", "product": "UPF0134 protein MPN_145", "uniref50_id": "UniRef50_P75141" }, "pscc": { "uniref50_id": "UniRef50_P75141", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75141" ], "product": "UPF0134 protein MPN_145" }, "genes": [], "id": "APKECPOBEN_377", "locus": "GCA002090215_00341" }, { "type": "cds", "sequence": "contig_1", "start": 193046, "stop": 193612, "strand": "+", "frame": 2, "gene": null, "product": "putative protein MPN_146", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75140", "UniRef:UniRef90_P75140" ], "nt": "ATGTTGGCTAGTTGTGGTGCTAAAGGGCGTTTTGACCAGGTTGATGATGGCAAAATTAAGCTAGCTTCTTCACTTACCGGTAAGCGCGATGTGATACTGCAAGAAGTGCTCAACAAATATAACAGTCGCAAAGCCAAGGACGATTATCCGATTGAAATAACCAAAATAGCCGGTAGCTATGATGGTGGTCGATCTGATCTGCAAACGCGGTTGAGTGTCAAGGATAAAACAACCTTTTACAACATGATCTTGAATTATTCTGACACCATTTCTACTTTAGGTCGCAGTAACATGGAACTACCATTAGATAGCGTTGACGTATCACAGTTTTCAGAAAACTTCTTGAGCTTTAATGACCGCATAAGTGGTATTTCCAGAAAAGGCATTTACGGCATTCCGGTATCAATGTCAACTGATATCCTAGTAATCAACGGGCCGGTATTGCACTATATTTTGAATAGTGCAAAAAAGAAAGATGGTGCAGTAACTAAGAAAAACGCAAGTAATTCCAATGGTAACGAAGGAACGTTAACAGTAAATAACGATCAACAAACCACTGAACTATGA", "aa": "MLASCGAKGRFDQVDDGKIKLASSLTGKRDVILQEVLNKYNSRKAKDDYPIEITKIAGSYDGGRSDLQTRLSVKDKTTFYNMILNYSDTISTLGRSNMELPLDSVDVSQFSENFLSFNDRISGISRKGIYGIPVSMSTDILVINGPVLHYILNSAKKKDGAVTKKNASNSNGNEGTLTVNNDQQTTEL", "aa_hexdigest": "0ece58117b962ef63f66cc72c0aa91b4", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 192994, "stop": 193843, "observations": { "insertion": [ 193042 ], "stop": [ 193610, 193772 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75140", "query_cov": 1.0, "subject_cov": 0.6643109540636042, "identity": 0.995, "score": 291.0, "evalue": 6.92e-97, "gene_start": 1, "gene_end": 188, "reference_start": 19, "reference_end": 206, "reference_sequence": "MRFKYGAVAFSGFLGLAVMLASCGAKGRFDQVDDGKIKLASSLTGKRDVILKEVLNKYNSRKAKDDYPIEITKIAGSYDGGRSDLQTRLSVKDKTTFYNMILNYSDTISTLGRSNMELPLDSVDVSQFSENFLSFNDRISGISRKGIYGIPVSMSTDILVINGPVLHYILNSAKKKDGAVTKKNASNSNGNEGTLTVNNDQQTTELWKKIEEAAKTNGKTTQEQTKRDAKQSTSLIQLKEGSANTTEGNASESDKEIKKSWGNYQEVDGGLKGYTFKASVFEN" }, "paralog": false, "identity": 0.9858657243816255, "score": 465.0, "evalue": 5.2e-168, "description": "Internal stop codon at 193610, 193772. Frameshift due to insertion around 193042.", "psc": { "uniref90_id": "UniRef90_P75140", "product": "Uncharacterized protein MPN_146", "uniref50_id": "UniRef50_P75140", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75140", "UniRef:UniRef50_P75140" ] }, "pscc": { "uniref50_id": "UniRef50_P75140", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75140" ], "product": "Uncharacterized protein MPN_146" } }, "id": "APKECPOBEN_378", "locus": "GCA002090215_00342" }, { "type": "cds", "sequence": "contig_1", "start": 193850, "stop": 194935, "strand": "+", "frame": 2, "gene": null, "product": "P68 family surface lipoprotein", "db_xrefs": [ "GO:0009897", "SO:0001217", "UniRef:UniRef50_P75139", "UniRef:UniRef90_P75139" ], "nt": "ATGGTTGATTTTGCCACTAGAGTAGCGAAATCATTTAAGGATAAAGTTAGTGGTATCGATAATAAAAAAGGCACTGACATCCAAGGTGTTTTAGGTCTAGACAGTACACCAAACGTTTTGTTCGCTTCAGTATTTGCAGCTGGTGGTGGTAGCTATGACAACTTCTTTTACAAGGTAGAAAATGGCCGGGCTGATTTCCGTAATTTTGCAAATAAAGGTACTTCTTACAAAAACTTAGAGAAGGTTTACAACGATTACAAGAATTTAATCGGTAGCAACGGTCTTTTTGCTAGCAAAGATGGCTCTTACTCATCTAACTTTGAAAAGTTCCACCAGTTAGCTTTTTATGTAGGTTCTAGCTCTGGTTATAACTATGCTTTTGCTGATGAAACTGCTAAACGGTTAAAGTTTGGTGATAGTGCCATTGAGTATCCTAACGATACGCTTGAAATTAAGGCACCGACAAATAATAGTCAAAGTGGTGATGGGAATGGTGGCACCAATAACGACAATCTCTTAGGTACTTTTGACATTAGGGAAAAGAGTAACGGCAAAAAAGGTGAGAGCAACGGTAAACAAGGAAATGGTCAAGATAAAAAGACAATTTCGCTTTATAAAGATTCGATTCCTAAAGAAAAAACTGAAGGCACTGATGCAATTCTAATTAGCGATAATCAACTCATTAATCAATTACAAACTGCCGCTAAAACTAGTAGTTCACAAAATAAAGCTAACACTGCAGCTATAACTTTTAAGCAAAGCAATAAAAGTGAAACTGACCAAAGTACAACTAGTCAGGTTATCGGCTACACAACAACTGCTTCATTGAAAGCTGATAAGAAAAATATCTTTGATGTGAAGAAGCTAAATAACGAAAAGTCTGAGCGCAAAATCATTGTCGGTGCCACGGTGGAAACCTTAAATCAAGCAAACACATTGCAAGCTAATGAAGCCATTATTAAACCAGCACCTGGTAAGTACCAAAGTACTGATTCACACAAGGTCATGATTACTCAAGGCCCGAATATTGTCGGAATTCATGCCAACGAAAAAGAGGACAAAGAAACACAAAAGTTTATTAACTGA", "aa": "MVDFATRVAKSFKDKVSGIDNKKGTDIQGVLGLDSTPNVLFASVFAAGGGSYDNFFYKVENGRADFRNFANKGTSYKNLEKVYNDYKNLIGSNGLFASKDGSYSSNFEKFHQLAFYVGSSSGYNYAFADETAKRLKFGDSAIEYPNDTLEIKAPTNNSQSGDGNGGTNNDNLLGTFDIREKSNGKKGESNGKQGNGQDKKTISLYKDSIPKEKTEGTDAILISDNQLINQLQTAAKTSSSQNKANTAAITFKQSNKSETDQSTTSQVIGYTTTASLKADKKNIFDVKKLNNEKSERKIIVGATVETLNQANTLQANEAIIKPAPGKYQSTDSHKVMITQGPNIVGIHANEKEDKETQKFIN", "aa_hexdigest": "387f1edd3222556d33033dee7e67179c", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 193850, "stop": 195238, "observations": { "mutation": [ 194933, 194957 ], "stop": [ 194933, 194957 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75139", "query_cov": 1.0, "subject_cov": 0.7443298969072165, "identity": 1.0, "score": 676.0, "evalue": 6.2e-243, "gene_start": 1, "gene_end": 361, "reference_start": 1, "reference_end": 361, "reference_sequence": "MVDFATRVAKSFKDKVSGIDNKKGTDIQGVLGLDSTPNVLFASVFAAGGGSYDNFFYKVENGRADFRNFANKGTSYKNLEKVYNDYKNLIGSNGLFASKDGSYSSNFEKFHQLAFYVGSSSGYNYAFADETAKRLKFGDSAIEYPNDTLEIKAPTNNSQSGDGNGGTNNDNLLGTFDIREKSNGKKGESNGKQGNGQDKKTISLYKDSIPKEKTEGTDAILISDNQLINQLQTAAKTSSSQNKANTAAITFKQSNKSETDQSTTSQVIGYTTTASLKADKKNIFDVKKLNNEKSERKIIVGATVETLNQANTLQANEAIIKPAPGKYQSTDSHKVMITQGPNIVGIHANEKEDKETQKFINWYLNKEESWSVQNSGSTTTKKQTAAQYFAEQSSYITPLKNNFKADQSATKDSTVNTNYFTKQTFDLFKEVNDGKVLGFNDPSDFRSGKFRNTIGSTFNATISSKVDFNKFFENFKASLGSGFER" }, "paralog": false, "identity": 0.9956709956709957, "score": 895.0, "evalue": 0.0, "description": "Internal stop codon at 194933, 194957. Nonsense mutation around 194933, 194957.", "psc": { "uniref90_id": "UniRef90_P75139", "product": "P68 family surface lipoprotein", "uniref50_id": "UniRef50_P75139", "go_ids": [ "GO:0009897" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75139", "UniRef:UniRef50_P75139", "GO:0009897" ] }, "pscc": { "uniref50_id": "UniRef50_P75139", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75139" ], "product": "Uncharacterized protein MPN_147" } }, "id": "APKECPOBEN_379", "locus": "GCA002090215_00343" }, { "type": "cds", "sequence": "contig_1", "start": 194963, "stop": 195307, "strand": "+", "frame": 2, "gene": null, "product": "putative lipoprotein MPN_098", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75594" ], "nt": "GTGCAAAACAGCGGTTCAACTACAACTAAAAAGCAAACTGCTGCTCAATATTTTGCCGAACAGTCCTCTTACATCACTCCTTTAAAAAATAACTTTAAAGCAGATCAAAGTGCAACAAAAGATAGCACTGTTAACACTAACTATTTCACTAAACAAACATTTGATCTCTTCAAAGAAGTTAATGATGGAAAAGTGCTTGGTTTTAATGATCCAAGTGACTTTAGGAGTGGTAAGTTCCGCAATACTATTGGTTCTACTTTTAACGCAACCATCAGTTCTAAAGTTGACTTTAACAAGTTTTTTGAAAACTTTAAAGCTTCTTTAGGGTCTGGTTTTGAGCGTTAA", "aa": "MQNSGSTTTKKQTAAQYFAEQSSYITPLKNNFKADQSATKDSTVNTNYFTKQTFDLFKEVNDGKVLGFNDPSDFRSGKFRNTIGSTFNATISSKVDFNKFFENFKASLGSGFER", "aa_hexdigest": "d4ca02fa10a084a1bd117b29b5139ba6", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0AAN4XG79", "query_cov": 0.9385964912280702, "subject_cov": 0.8492063492063492, "identity": 0.523, "score": 110.0, "evalue": 4.69e-29, "valid": false, "product": "Lipoprotein", "uniref50_id": "UniRef50_P75594" }, "pscc": { "uniref50_id": "UniRef50_P75594", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75594" ], "product": "Uncharacterized lipoprotein MPN_098" }, "genes": [], "id": "APKECPOBEN_380", "locus": "GCA002090215_00344" }, { "type": "cds", "sequence": "contig_1", "start": 195480, "stop": 195764, "strand": "+", "frame": 3, "gene": null, "product": "putative lipoprotein MPN_467", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75316" ], "nt": "ATGCACAAAAAGAAGTTTTTATCAAGAACTTCCTTTGCTTCTTTGTTTTTGCTTTTAGGAACAGTATTGAGTGCTTGTAGCAAAGTAGAGTCCACAAAAAGTGTTCATCCTGTAAAAAGTTTTAAATCTGATCTAAAAAGTCTTCTTAAAGAAACAATTAAACAAGATATTGATTTATCTAAAACAATCAAAACTTCAGAAGGCAAACAACAGATAATAACTTCTTTAAAAAAGTCTTACGAAGTTAATCCGAAAGATACAACAAAATTGTTGTTAGACGCATGA", "aa": "MHKKKFLSRTSFASLFLLLGTVLSACSKVESTKSVHPVKSFKSDLKSLLKETIKQDIDLSKTIKTSEGKQQIITSLKKSYEVNPKDTTKLLLDA", "aa_hexdigest": "12903cfa0473d88965b8de7593827d95", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75316", "query_cov": 1.0, "subject_cov": 0.9306930693069307, "identity": 0.617, "score": 102.0, "evalue": 1.16e-26, "valid": false, "product": "Uncharacterized lipoprotein MPN_467", "uniref50_id": "UniRef50_P75316", "go_ids": [ "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75316", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75316" ], "product": "Uncharacterized lipoprotein MPN_467" }, "genes": [], "id": "APKECPOBEN_381", "locus": "GCA002090215_00345" }, { "type": "cds", "sequence": "contig_1", "start": 195849, "stop": 195938, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAGCGAAAGGTTGATCATTTCCAAATGACATACCAAAGTTTTAAGGATTTATCAATTAAAGCTAAGTTGAGCTATACCTTTAATTGA", "aa": "MERKVDHFQMTYQSFKDLSIKAKLSYTFN", "aa_hexdigest": "893f67d2558641e21e4c66ec2ae765db", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3557.0605000000005, "isoelectric_point": 9.302803993225098 }, "id": "APKECPOBEN_382", "locus": "GCA002090215_00346" }, { "type": "cds", "sequence": "contig_1", "start": 196591, "stop": 196797, "strand": "+", "frame": 1, "gene": null, "product": "Family K-like protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAP8E4D4", "UniRef:UniRef90_A0AAP8E4D4" ], "nt": "ATGATAGAAGTATTAAGTGATTCTGAAATTTTATCTTATTTATTAAGTAATAAATACACTGCTGATGGAGTTAATGAAATTTATGTAACGATGAACATTAAAGACAAAGATTCAAACACTCAAAGGAAGCCAGATAAAATGTCTTTAGAGCTTGAATTGTTTTTAAATCAGTTTATTGAATTCTTAAAAGAGCAGAGAAAAAGCTAG", "aa": "MIEVLSDSEILSYLLSNKYTADGVNEIYVTMNIKDKDSNTQRKPDKMSLELELFLNQFIEFLKEQRKS", "aa_hexdigest": "1c2b98a315da9777c8492c7fded3d0bd", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0AAP8E4D4", "query_cov": 1.0, "subject_cov": 0.8095238095238095, "identity": 1.0, "score": 130.0, "evalue": 3.71e-38, "valid": true, "product": "Family K-like protein", "uniref50_id": "UniRef50_A0AAP8E4D4" }, "pscc": { "uniref50_id": "UniRef50_A0AAP8E4D4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAP8E4D4" ], "product": "Family K-like protein" }, "genes": [], "id": "APKECPOBEN_383", "locus": "GCA002090215_00347" }, { "type": "cds", "sequence": "contig_1", "start": 196887, "stop": 197456, "strand": "+", "frame": 3, "gene": null, "product": "Putative MgpC-like protein MPN_149", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75037", "UniRef:UniRef90_P75037" ], "nt": "GTGGGTGATACCAAGTTGGTGGCGCTGGTCCGCTCAGCGTTGGTCAGGGTGAAGTTTAATGATGGGACGAGTTCGGATTCTAATAACCAAGATACGAATCAAAATGCCTTGAGTTTTGATACCCAAGAATCACAGAAGGCACTTAATGGCTCGCAGAGTGGATCTTCTGACACTTCCGGGTCTAACTCCCAAGACTTCGCCAGCTACATCCTCATCTTCCAAGCCGCGCCCAGGGCCACGTGGGTGTTTGAACGGAAGATTAAGTTAGAGTTGCCCTACGTTAAAAACGAATCGGGAGCAGGGGACTCCACCACAACTAACAGTGGCTCCCTCTACACCACCCTCCAAGACCTCCTCGTCGAACAACCCGTGACCCCTTACACGCCCAATGCGGGGTTAGCCCGGGTGAATGGGGCTGCTCAGGATACGGTTCATTTTGGTTCGGGTCAAGAATCGAGTTGGAATTCCCAACGTTCCCAAAAAGTCCTTAAAAACAACCCCGGACCCAAAGCCGTCACCGGCTTTAAGCTCGATAAGGGCCGCGCGTACCGGAAGCTAAATGAAGCTTGA", "aa": "MGDTKLVALVRSALVRVKFNDGTSSDSNNQDTNQNALSFDTQESQKALNGSQSGSSDTSGSNSQDFASYILIFQAAPRATWVFERKIKLELPYVKNESGAGDSTTTNSGSLYTTLQDLLVEQPVTPYTPNAGLARVNGAAQDTVHFGSGQESSWNSQRSQKVLKNNPGPKAVTGFKLDKGRAYRKLNEA", "aa_hexdigest": "ae619ba2e3ccef14a91bc9d578fc2c9e", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 196824, "stop": 197759, "observations": { "mutation": [ 196878, 197454 ], "stop": [ 196878, 197454 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75037", "query_cov": 1.0, "subject_cov": 0.43548387096774194, "identity": 0.995, "score": 356.0, "evalue": 1.55e-120, "gene_start": 1, "gene_end": 189, "reference_start": 22, "reference_end": 210, "reference_sequence": "MAVGIFILSLNPSYELVDWKRVGDTKLVALVRSALVRVKFNDGTSSDSNNQDTNQNALSFDTQESQKALNGSQSGSSDTSGSNSQDFASYILIFQAAPRATWVFERKIKLELPYVKNESGAGDSTTTNSGSLYTTLQDLLVEQPVTPYTPNAGLARVNGAAQDTVHFGSGQESSWNSQRSQKVLKNNPGPKAVTGFKLDKGRAYRKLNEAWPVYEPLDSTKDGKGKDESSWNSSEKTTAESDAPLVGSTGSQMAAVTDSQQSGDNNGLVSLAQRSTTVAVQKSDSSGSQGQGTTDNKFQKYLNTTQALHQMGVIVPSLETWAGENKYWNRYPCCWWCFSPSSDPAIVFHKWRSTECNHPVIPYFNRPTRLLKWPDRCDGFERGTEFVVLGRGWANHVRKGYLMSPHRVELGHRQAEGVCGESAWFQWNQRHRLA" }, "paralog": false, "identity": 0.9935691318327974, "score": 604.0, "evalue": 1.55e-220, "description": "Internal stop codon at 196878, 197454. Nonsense mutation around 196878, 197454.", "psc": { "uniref90_id": "UniRef90_P75037", "product": "Putative MgpC-like protein MPN_149", "uniref50_id": "UniRef50_P75037", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75037", "UniRef:UniRef50_P75037" ] }, "pscc": { "uniref50_id": "UniRef50_P75037", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75037" ], "product": "Putative MgpC-like protein MPN_149" } }, "id": "APKECPOBEN_384", "locus": "GCA002090215_00348" }, { "type": "cds", "sequence": "contig_1", "start": 197460, "stop": 197876, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTATGAACCCCTGGATTCGACCAAGGATGGCAAGGGGAAGGATGAGAGCTCTTGGAACAGTTCGGAAAAAACAACGGCGGAAAGTGATGCCCCGTTGGTGGGATCAACCGGGAGTCAAATGGCTGCAGTCACAGACAGTCAGCAATCAGGTGATAACAATGGATTGGTATCGCTTGCCCAGCGCTCCACTACTGTTGCTGTCCAAAAAAGTGACTCTTCTGGTTCTCAAGGCCAAGGCACAACCGATAACAAATTCCAAAAATACCTCAACACCACCCAGGCCCTGCACCAGATGGGGGTGATAGTGCCATCTTTGGAAACGTGGGCGGGGGAAAACAAGTACTGGAATCGCTACCCGTGCTGCTGGTGGTGTTTCAGTCCAAGCAGCGACCCGGCAATCGTCTTCCACAAATGA", "aa": "MYEPLDSTKDGKGKDESSWNSSEKTTAESDAPLVGSTGSQMAAVTDSQQSGDNNGLVSLAQRSTTVAVQKSDSSGSQGQGTTDNKFQKYLNTTQALHQMGVIVPSLETWAGENKYWNRYPCCWWCFSPSSDPAIVFHK", "aa_hexdigest": "1088188cd4fd0a9a1f87456e227f457c", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14993.192999999997, "isoelectric_point": 5.013051414489746 }, "id": "APKECPOBEN_385", "locus": "GCA002090215_00349" }, { "type": "cds", "sequence": "contig_1", "start": 198046, "stop": 198600, "strand": "+", "frame": 1, "gene": "mgpC", "product": "MgpC family cytadherence protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002246AD21" ], "nt": "TTGAACTGGGGCACCGACAAGCAGAAGGAGTTTGTGGAGAATCAGCTTGGTTTCAATGAAACCAGCGCCACCGACTCGCATAACTTTAAAAAAGCGCTGTTACATCAACCCGCCTACCTCATCAGTGGCCTTGACGTTGTGGCCGACCACCTCGTCTTTGCGGCCTTTCGAGCCGGCGCGGTGGGGTATGATATGACGACTGATACGAACGCTTCGACCTACAACCAAGCGCTCACCTGGTCGACCACGGCCGGGTTGGACAGTGCTGGGGGGTACAAGGCCTTGGTGGAAAACACGGCCGGGCTCAACGGCCCGATTAACGGCTTGTTTACCCTGCTCGACACCTTTGCGTATGTGACCCCGGTGAGTGGGATGAAAGGGGGGAGTAAGAATACGGAGGCAGTGCAAACGACTTATCCGGTCAAGTCCGACCAAAAGGCTTCCGCCAAAATTGCGTCCTTAATTAATGCCAGTCCACTCAACAGTTATGGGGATAAAAAAAGCCATCCACCTAAGGTGAAAGGCAGACAGCTCCAAAATATTTTTGACGATTGA", "aa": "MNWGTDKQKEFVENQLGFNETSATDSHNFKKALLHQPAYLISGLDVVADHLVFAAFRAGAVGYDMTTDTNASTYNQALTWSTTAGLDSAGGYKALVENTAGLNGPINGLFTLLDTFAYVTPVSGMKGGSKNTEAVQTTYPVKSDQKASAKIASLINASPLNSYGDKKSHPPKVKGRQLQNIFDD", "aa_hexdigest": "ebb441199d47eb2d0462a23a5d0b4aa5", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001FCE6DDF", "query_cov": 1.0054347826086956, "subject_cov": 0.8409090909090909, "identity": 0.789, "score": 279.0, "evalue": 2.59e-93, "valid": false, "product": "MgpC family cytadherence protein", "uniref50_id": "UniRef50_UPI002246AD21" }, "pscc": { "uniref50_id": "UniRef50_UPI002246AD21", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002246AD21" ], "product": "MgpC family cytadherence protein" }, "genes": [ "mgpC" ], "id": "APKECPOBEN_386", "locus": "GCA002090215_00350" }, { "type": "cds", "sequence": "contig_1", "start": 199032, "stop": 199514, "strand": "+", "frame": 3, "gene": null, "product": "UPF0134 protein MPN_151", "db_xrefs": [ "RefSeq:WP_015344885.1", "SO:0001217", "UniParc:UPI0002B8489E", "UniRef:UniRef100_P75035", "UniRef:UniRef50_P75490", "UniRef:UniRef90_P75035" ], "nt": "TTGTTTAAGAAAAGACTCAACAAAGATAAGATTAATAATTGTCATGTTTGGGAAGAAGAGTTACCTGATGGTAGCTACGACATGGAATTTAATGGCAATTTAAACCATATGGAAAAACGAAAAAGTGGTTATGTTACCCAAAAGCAGTTTAACGAATATAAAGACTCAAACGACCAACGGCTCATAAAGATTGAAACTACTTTGGCTGCCCAAGGTGAGCAAATTAGTCAGCTAGTTCAAATTGTGTTCCTCCAAGGAAAACAAATTAAAGAACTTCAAGCGGAGCAAAAAGCACAAAGAGTTGAATTTAACGCGCGCATGGATCGCTTTGAAAGCCTTGTGCTTAAGAGTCTGGAATCAATCGGAAACACTTTGACTGATTTTGGTAAACGATTCGATTCAATGGAAACTCGTTTGGACTCTATGGATGGTCGTCTCGATTCAATGGAAACTCGTTTAGACAAAATCGATCCACCTAAATAG", "aa": "MFKKRLNKDKINNCHVWEEELPDGSYDMEFNGNLNHMEKRKSGYVTQKQFNEYKDSNDQRLIKIETTLAAQGEQISQLVQIVFLQGKQIKELQAEQKAQRVEFNARMDRFESLVLKSLESIGNTLTDFGKRFDSMETRLDSMDGRLDSMETRLDKIDPPK", "aa_hexdigest": "95262681e84cf2ed0dcb64c927c055ed", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0002B8489E", "ncbi_nrp_id": "WP_015344885.1", "uniref100_id": "UniRef100_P75035", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002B8489E", "RefSeq:WP_015344885.1", "UniRef:UniRef100_P75035" ] }, "ips": { "uniref100_id": "UniRef100_P75035", "uniref90_id": "UniRef90_P75035" }, "psc": { "uniref90_id": "UniRef90_P75035", "product": "UPF0134 protein MPN_151", "uniref50_id": "UniRef50_P75490" }, "pscc": { "uniref50_id": "UniRef50_P75490", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75490" ], "product": "UPF0134 protein MPN_287" }, "genes": [], "id": "APKECPOBEN_387", "locus": "GCA002090215_00351" }, { "type": "cds", "sequence": "contig_1", "start": 199797, "stop": 200447, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATTTAAGTACGGCGCTATTGTTTTTAGTGGTCTTTTGGGCGTTTCGGCCATTTTAGCTGCCTGTGGGGCAAGGGGCAAATTTAACCAAGTTGATGATGGCAAGATTAAGCTAGCTTCATCTTTAACTTCCAGAAGTGCGTCAACAGCTTTACAAAAAGTTGTTGAAAAGTATAACAAGGTTAAAGGCGTAAATGATTACCCCATTGAAATTACCCAAATTGCTGGCGGTTATGATGGCGGGCGGACTGATCTACAAACCCGAGTTAACGTTAAAGACACGACTAATTTTTATAACCTCATCTTGAACTATCCCGACTTAGTTTCCACTTTGGGACGTGTTGGGATGGAACTACCTTTTGATAAGGTTAAAACTGATAAGTTAGCTGACCGTTTTTTGGATTTCAACAACCGTATTAGTGCCATTTCCAAAAAGGGCATTTATGGCATTCCGGTTTCTTTATCCACCGAAGTTTTATCGATTAATGGACCCGTTTTGCACTATATTTTGAATAGTGCACAAGGAAAGGTTAGTTCTGTCACTTCGGTGCAAAGAACAGCAGATTCTGGTAGTGGAACAACAAATAATAATGGAGTAACAAAACCTTTGAAGATTGATAAAGAAAATGAATCTACAAAGAAACTTTGA", "aa": "MKFKYGAIVFSGLLGVSAILAACGARGKFNQVDDGKIKLASSLTSRSASTALQKVVEKYNKVKGVNDYPIEITQIAGGYDGGRTDLQTRVNVKDTTNFYNLILNYPDLVSTLGRVGMELPFDKVKTDKLADRFLDFNNRISAISKKGIYGIPVSLSTEVLSINGPVLHYILNSAQGKVSSVTSVQRTADSGSGTTNNNGVTKPLKIDKENESTKKL", "aa_hexdigest": "62ae74a77bc6624d2d7cdfa1e2f9c801", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 23286.315700000014, "isoelectric_point": 9.593878746032715 }, "id": "APKECPOBEN_388", "locus": "GCA002090215_00352" }, { "type": "cds", "sequence": "contig_1", "start": 200532, "stop": 200675, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGCACTCAAACTCAACCTACTGACAATAGTAACGATGCTAATCAAAGCGACCAGGCGATAGAAAAGACTTGGGGTAAATATCAGGAGGTTGAAGGTGGTTTAAAAGATTACACCTTCAAAGCCAGTGTGTTTGAAAACTGA", "aa": "MSTQTQPTDNSNDANQSDQAIEKTWGKYQEVEGGLKDYTFKASVFEN", "aa_hexdigest": "20fa1cb098c572dd2efe284f89084f46", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5302.5751, "isoelectric_point": 4.285114479064942 }, "id": "APKECPOBEN_389", "locus": "GCA002090215_00353" }, { "type": "cds", "sequence": "contig_1", "start": 200682, "stop": 201878, "strand": "+", "frame": 3, "gene": null, "product": "P68 family surface lipoprotein", "db_xrefs": [ "GO:0005886", "GO:0009897", "SO:0001217", "UniRef:UniRef50_P75280", "UniRef:UniRef90_P75034" ], "nt": "TTGCTTGACTTTTCTAATAGGGTAGCTAAGTCATTTAGCAATATAAACACTAACACCAATAAAAAGGGCACCGATATTCAAGGTGTTCTGGGAATCGATAGCACTCCTAACTCCTTATTTACCTCTGTTTTTGCTGCAGGTGGCGGTAACTATGACAACTTCTTTTATAAGGTTGCAAACGGTCGGGCTGACTTTAGTAACTTTAAGAATAAAGGCACTTCTTACCAAAACTTACAAAAAGTTTATGGTGATTACAAAGGCTTGATTGACAGCAATGGTCTCTTTGTCAATAAGGGTGGTTCTTATTCATCGAACTTCCAAAAGTTTCACCAATTAGCTTACAGTATTTCCTCGACTTCCGGCTTCTTCTACTCGTTTGCTGGTGAAAACTCAAAGCGCTTGAAGTTTGATGGCGGTAATTTTATTGAGTTTCCTGGATTTACTCAAGCAATTTATGCGCCTGAAAATAATAGTCAAAGTGGTGGAGAGAGTGGCGATCAAACCACAAATAATGAAGCCAATCTTTTAGGCACTTTCAAAATAAAAAGCTCTAATAAATCTAAATCTTCAAGTTCCAAATCAAGCACTAAAGCCGAAACAGGCAAAACTAGTGGTGGAGACCAAAACCAAGGCAAAAAAGGCGAAGGCGCTCAAAATCAAGGCAAAAAAGGCGAAGGCGCTCAAAATCAAGGCAAAAACGGAGGTGAAGAAACAACTATTTACCTTTATAAAAACTCAATTCCATCTGGAAAAAATAAAGGCGAAAATGCAATTCTTATAGATAACAAAACTATTGTGGAACAACTAGAAAGTGCGGCTAAAAAAGAAGATAAGAAGAGTGGCGAATCTACCACAGAACAGACACAAATACAAAGTAAAAGTGTAACAGAACAAAAACAACCCAAAATCATCGGTTACACTACCACTGGCAATGTTCACGAAGACAACAAACACATTTTCCCAATTGACAAACTTACATCTGATCGAAATTTTGACCGCAAAATTATTGTTGGTGCCACTGAAGAAACACTCGATAAATCGAATACTTTGCAGTCCAATGAAGCCATTGTTTTACCAGCACCCTCTAAGTACAAAAGTACTGACACTAACAAAGTAACCATCACCCAAGGTCCGAATATCATTGGAATTCACGCCAACGAAAAGGAAAATGCAGAAACACAAAAGTTTGTGGATTGA", "aa": "MLDFSNRVAKSFSNINTNTNKKGTDIQGVLGIDSTPNSLFTSVFAAGGGNYDNFFYKVANGRADFSNFKNKGTSYQNLQKVYGDYKGLIDSNGLFVNKGGSYSSNFQKFHQLAYSISSTSGFFYSFAGENSKRLKFDGGNFIEFPGFTQAIYAPENNSQSGGESGDQTTNNEANLLGTFKIKSSNKSKSSSSKSSTKAETGKTSGGDQNQGKKGEGAQNQGKKGEGAQNQGKNGGEETTIYLYKNSIPSGKNKGENAILIDNKTIVEQLESAAKKEDKKSGESTTEQTQIQSKSVTEQKQPKIIGYTTTGNVHEDNKHIFPIDKLTSDRNFDRKIIVGATEETLDKSNTLQSNEAIVLPAPSKYKSTDTNKVTITQGPNIIGIHANEKENAETQKFVD", "aa_hexdigest": "0eab26311ca660acbccde69ba9c1b775", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 200382, "stop": 202180, "observations": { "deletion": [ 202143 ], "mutation": [ 200445, 200673, 201876, 201900 ], "stop": [ 200445, 200673, 201876, 201900 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75034", "query_cov": 1.0, "subject_cov": 0.4830097087378641, "identity": 0.997, "score": 697.0, "evalue": 1.61e-245, "gene_start": 1, "gene_end": 398, "reference_start": 296, "reference_end": 693, "reference_sequence": "MKFKYGAIVFSCLLGVSAILAACGARGKFNQVDDGKIKLASSLTSRSASTALQKVVEKYNKVKGVNDYPIEITQIAGGYDGGRTDLQTRVNVKDTTNFYNLILNYPDLVSTLGRVGMELPFDKVKTDKLADRFLDFNNRISAISKKGIYGIPVSLSTEVLSINGPVLHYILNSAQGKVSSVTSVQRTAASGSGTTNNNGVTKPLKIDKENESTKKLWEEIENKAKENGKKTSSSRRKRNLSSSKQMSTQTQPTDNSNDANQSDQAIEKTWGKYQEVEGGLKDYTFKASVFENWHDLLDFSNRVAKSFSNINTNTNKKGTDIQGVLGIDSTPNSLFTSVFAAGGGNYDNFFYKVANGRADFSNFKNKGTSYQNLQKVYGDYKGLIDSNGLFVNKGGSYSSNFQKFHQLAYSISSTSGFFYSFAGENSKRLKFDGGNFIEFPGFTQAIYAPENNSQSGGESGDQTTNNEANLLGTFKIKSSNKSKSSSSKSSTKAETGKTSGGDQNQGKKGEGAQNQGKKGEGAQNQGKNGGEETTIYLYKNSIPSGKNKGENAILIDNKTIVEQLESAAKKEDKKSGESTTEQTQIQSKSVTEQKQPKIIGYTTTGNVHEDNKHIFPIDKLTSDRNFDRKIIVGATEETLDKSNTLQSNEAIVLPAPSKYKSTDTNKVTITQGPNIIGIHANEKENAETQKFVDWFLNSPQTWEGKGKGKEQTNKTAAEFFAESASYILPLKEIFEQKNEKKEGSDQKDSKSNGRGKQTNLYTEKALELFRGISTDQIVSYSDPSDFRSGRFRDGIGATFNAAVSSKASFEKFVQNFIATLGSDI" }, "paralog": false, "identity": 0.993322203672788, "score": 1108.0, "evalue": 0.0, "description": "Internal stop codon at 200445, 200673, 201876, 201900. Frameshift due to deletion around 202143. Nonsense mutation around 200445, 200673, 201876, 201900.", "psc": { "uniref90_id": "UniRef90_P75034", "product": "P68 family surface lipoprotein", "uniref50_id": "UniRef50_P75280", "go_ids": [ "GO:0005886", "GO:0009897" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75034", "UniRef:UniRef50_P75280", "GO:0005886", "GO:0009897" ] }, "pscc": { "uniref50_id": "UniRef50_P75280", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75280" ], "product": "Uncharacterized lipoprotein MPN_506" } }, "id": "APKECPOBEN_390", "locus": "GCA002090215_00354" }, { "type": "cds", "sequence": "contig_1", "start": 201978, "stop": 202181, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCACTCAAGGAAATTTTTGAGCAAAAGAATGAAAAGAAAGAAGGCAGCGATCAAAAAGATAGTAAAAGCAATGGTAGAGGTAAACAAACTAACCTCTACACCGAAAAGGCCTTGGAACTATTTAGAGGTATTAGTACCGATCAAATAGTAAGTTACAGTGACCAAGTGACTTTAGGAGTGGTCGTTTCCGTGATGGAATAG", "aa": "MPLKEIFEQKNEKKEGSDQKDSKSNGRGKQTNLYTEKALELFRGISTDQIVSYSDQVTLGVVVSVME", "aa_hexdigest": "5c092c4dbaaafdffd6bbb5a6708c9070", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7553.405600000002, "isoelectric_point": 5.416039848327636 }, "id": "APKECPOBEN_391", "locus": "GCA002090215_00355" }, { "type": "cds", "sequence": "contig_1", "start": 202591, "stop": 202809, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTGGCATTGAAGGTAATGTTTACAAACACTTCCAAACTGAACTTAGTCGCTTTTACAAGCAAACGCGCAAACACTTTCGCGAAACTGGTTCGGAAAGTCTCTTTTTAGGTTTACCAGTAATTGAGGGCATTAACGAGTTTAACGATGTCTTTCGTGCTCCTTTACTCTATGTGGGGGTTAAGTTAAAAGTAGCACCACGACTTGAACGCTTTTGA", "aa": "MFGIEGNVYKHFQTELSRFYKQTRKHFRETGSESLFLGLPVIEGINEFNDVFRAPLLYVGVKLKVAPRLERF", "aa_hexdigest": "d192c8883fe593f4effd274614fb1862", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8465.699599999993, "isoelectric_point": 9.518192863464353 }, "id": "APKECPOBEN_392", "locus": "GCA002090215_00356" }, { "type": "cds", "sequence": "contig_1", "start": 202810, "stop": 203733, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13086.12" ], "nt": "TTGGAAATAAACCGCGAGGAAATCTTTTTAAATCCGACCATCATCGGGGTTGAAATTAACAAGCGTAACAGCTTGTTTAAAAATAACTATGACACCACTAAGGTTGATATTAACCAGGCATTGGAAATCTTTCGTGAGTTGGAATACCAATTTCGGATGCCACTCACCTCGGAGTTAAAAAGTTTTTCCAAAAAGGCTAAGAGTGACTTTAACACTGAAAAACGGACTAACTTCCTCACTAATAACGTTCTTTTAGGAATCTTTGACGTTAAGGGGGATCAACTGTTCCAAAACTTTAACGAAATTCTAAACACTGATCCTGATGTTTTGGATGAATTGTTAAAGGATCGCCGCGATCTCTTATACGACAACCGTGAGTTTCGTGAAAACTTTAACTTAAAGGGCACTTACCTCTTTAGCCACTTAGACATCTTCCAGCAGTACGCTGTCAAACAGGCATTTGATGGGGATGTCATTATTGAAGGTCCACCTGGTACTGGTAAGAGTGAAACGATTGTCAACATCTTGGTCAACCTCGCTTTAAACAAAAAGAAGGTGTTGTTTGTGTCAGAAAAGGTAACTGCCTTAGATGTCGTTTACAACCGGCTAGGTAGCTTTAAGCACATTGCCTTATTTAATGCTTCGGTAGCGAGTGAGAAGAAGCGCTTTTACAGCCAGTTCGCTGATTATGAAAGCTTCTTTACCGATAACTTTTCCAAGAAGGATTTAGTTAACGAAATGCCGGTGTTTGACGGGCAGTGGGTTGATAAGATCCTAAGTGAATTCACTAACCTTCAAAACATTTACGATACCCAAATTAATTCGGGTAACCAGAGCTATTCCTTCAAGGAAATCCTAAGTAGTTTTCCAATACTTGATGTCAGTTACATTAAGATCAAGGAACACGATCGTTTTGATGAATGA", "aa": "MEINREEIFLNPTIIGVEINKRNSLFKNNYDTTKVDINQALEIFRELEYQFRMPLTSELKSFSKKAKSDFNTEKRTNFLTNNVLLGIFDVKGDQLFQNFNEILNTDPDVLDELLKDRRDLLYDNREFRENFNLKGTYLFSHLDIFQQYAVKQAFDGDVIIEGPPGTGKSETIVNILVNLALNKKKVLFVSEKVTALDVVYNRLGSFKHIALFNASVASEKKRFYSQFADYESFFTDNFSKKDLVNEMPVFDGQWVDKILSEFTNLQNIYDTQINSGNQSYSFKEILSSFPILDVSYIKIKEHDRFDE", "aa_hexdigest": "aacb909093bef0fa201f8ade522348c4", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13086.12", "name": "AAA_11", "length": 73, "aa_cov": 0.20846905537459284, "hmm_cov": 1.0, "evalue": 1.29927683317233e-09, "score": 33.647674560546875, "start": 142, "stop": 205 } ], "seq_stats": { "molecular_weight": 35840.027000000024, "isoelectric_point": 5.108881950378417 }, "id": "APKECPOBEN_393", "locus": "GCA002090215_00357" }, { "type": "cds", "sequence": "contig_1", "start": 203875, "stop": 204108, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTGTGCTATGTGTTGGATTACTTCGAGCAGAACAACAGCATAATCAAACCGAAGCGGGTATTGCTCTATACCCCCACTGAACGCGGGCAAAAGCAGTTGCACCAACTCCAGCAAGACGTTGCTAAGTACAACAGCTTGCAACGTTTTAAATCGGCTGCTAAGTTTGAAACAATTAAGCTCAATTTAGCTAACAAACTAGCACAAAACGCCAAGCCTTTTTTCTTTTCTTGA", "aa": "MLCYVLDYFEQNNSIIKPKRVLLYTPTERGQKQLHQLQQDVAKYNSLQRFKSAAKFETIKLNLANKLAQNAKPFFFS", "aa_hexdigest": "b38c3cda4b3cce2177ce1bfc3b606855", "start_type": "GTG", "rbs_motif": "AAAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9069.429899999994, "isoelectric_point": 9.812168693542482 }, "id": "APKECPOBEN_394", "locus": "GCA002090215_00358" }, { "type": "cds", "sequence": "contig_1", "start": 204136, "stop": 204828, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13086.12" ], "nt": "TTGGAAAATCTCGTACAAACCCAAAAGCAATTGGTTAAGGCTAAGCAAAGCTACTTAAGCAAAATTGAGCAGTATGTAGTTAGTTGTAAACGGATTCTCAAAGCTACCATTTTAGCCAACTTCTTTGAGTTGTACCAAACCAATAAGAATGAACTATTGGATATCTGTCGGGAAGCCAAAAACCCTGTTTTAAAGGAAATTACCTGGTGGTTTAAGAAGAACTTTGCACTGTTATCAAAGCTATTCCCCGTCCACATTATGACGTTTGAATCAGCCGCCTTATTAACCCCGAACCAGCGCCGTTTGTATGACTATGTGGTGATTGATGAAGCTAGCCAGGTTTATCTAGAACGTGCCATTCCAATCTTATACCGTGGCGCTAAGTACATTATTGCTGGAGACACCAAACAATTAAAACCATCCAATTTCTTCCAAGCCCGGGCTGAGTATGATGTGGATGAAGAGTTTGAAGATGGTAACGTTGAAGCTGCGGTGCACTCGACTTCGTTACTACACTTTTTGAAGAACCGTTCGCGCATTTTAACGCTGCTCAAATTCCACTACCGCAGTGATTCCGCTAACCTAATTGCCTTTACTAATAACCGCATTTATAACAACGAGCTCATCTTTATGAATAAAGCAACCGCTGACAAGCAGGTCTTTATTGTCCATGATGTCATCGATGGTATTTGA", "aa": "MENLVQTQKQLVKAKQSYLSKIEQYVVSCKRILKATILANFFELYQTNKNELLDICREAKNPVLKEITWWFKKNFALLSKLFPVHIMTFESAALLTPNQRRLYDYVVIDEASQVYLERAIPILYRGAKYIIAGDTKQLKPSNFFQARAEYDVDEEFEDGNVEAAVHSTSLLHFLKNRSRILTLLKFHYRSDSANLIAFTNNRIYNNELIFMNKATADKQVFIVHDVIDGI", "aa_hexdigest": "d305f28155a62318e0b718863ee1659c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13086.12", "name": "AAA_11", "length": 42, "aa_cov": 0.1782608695652174, "hmm_cov": 1.0, "evalue": 1.2268073756689257e-09, "score": 33.72935485839844, "start": 100, "stop": 140 } ], "seq_stats": { "molecular_weight": 26809.62560000002, "isoelectric_point": 8.885886955261231 }, "id": "APKECPOBEN_395", "locus": "GCA002090215_00359" }, { "type": "cds", "sequence": "contig_1", "start": 205009, "stop": 205620, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13087.12" ], "nt": "GTGAACGCTCAGGGCGAGTATGTCGGTTTGTTTGTCAAAAACATTGAAAACGTTCAGGGTGATGAACGGGACATTATTATCTTCTCGTTGGGCTATGACCGTTCTGTCAACAGCTATGGCCCGATTTCCAAGCAAGGTGGGGAGAACCGTTTGAATGTAGCGATTACCCGTGCTAAGCAGCGGATTGAACTGTTCAAGACTAACCGTGCTAGTGATTACAATGGTTTGAGCTCCAATTCCTTGGGTAGTAAGTTATTGGTGGAATACTTACTGTACTGTGAGGCAATGGCCAACAACCAGGGTGAGAGCTTAGACTTCCAAGCAACCCAAAAACAAGCACCCAAGGCCAAGTACGAATTGGAATTGGAGAACCAGTTCTTTAATGAGTTGGAACTTATTTTTGGTGAGCAGTTTACGATTAAACGCAATGTCAATGAGGGTGCATACAGCTTTAGCTTTGTCTTTTACTTTAACGAAAACCCTTACCTAGCGGTTGACTTTAACCCTGCTTTACCCCACTCCAGAAAGGAAGTGAGTGAAAACATTATTTACCGCGAACAATTTTTGAAAAAGCGGAAATGGAACCTCGTTAACATTTGGTTAGACGAGTGA", "aa": "MNAQGEYVGLFVKNIENVQGDERDIIIFSLGYDRSVNSYGPISKQGGENRLNVAITRAKQRIELFKTNRASDYNGLSSNSLGSKLLVEYLLYCEAMANNQGESLDFQATQKQAPKAKYELELENQFFNELELIFGEQFTIKRNVNEGAYSFSFVFYFNENPYLAVDFNPALPHSRKEVSENIIYREQFLKKRKWNLVNIWLDE", "aa_hexdigest": "542c68ba3f61a75ff8891bc9036adde9", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13087.12", "name": "AAA_12", "length": 53, "aa_cov": 0.26108374384236455, "hmm_cov": 0.9433962264150944, "evalue": 1.038890205005435e-09, "score": 33.67409133911133, "start": 10, "stop": 62 } ], "seq_stats": { "molecular_weight": 23528.12060000002, "isoelectric_point": 5.281274604797362 }, "id": "APKECPOBEN_396", "locus": "GCA002090215_00360" }, { "type": "cds", "sequence": "contig_1", "start": 205775, "stop": 206395, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08529.16" ], "nt": "ATGAACAATCAATCAAATCACTTTACCAATCCTTTGTTACAACTGATTAAGAACGTTGCCGAGACCAAGAATTTAGCCATCGATGATGTTGTTTTGTGTCTTAAAACAGCATTAGCACAAACATACAAGAAGCATTTGAACTATGTCAATGTGGAAGTCAACATTGACTTTAACAAGGGCTTAATGCAAATTGAACAGCTCTTCGATGTTGTAGATGATAACAATGAGGATTATGATGACTTTTTGGAAATGCCTTTAAGTGAGGCTAAGAAGCTCAATCCGAACCTAGAAGTTGGCGGGGTGTTGCGCAAACCGGTTTCACTCAAAGACATTAAGGGCGATCTCATTAGCAAAATGGTATTACTCTTCAACCAAAAGATTAACGAAACAGCTTTTAAAACGGTGATGAGTGACTTCATTAATGAAGTCGGTCAGGTAATTGAAGCTAGAGTAGAGGACATTGATACGAACAAAGATGGTGGATTGAAGGGTTACATTGTTAACTTGGAAACCACCAAGGGTTACATGCCTAAACGTGAGTTGAGTAAGGGCGAAAAGCTAGATATTGGCAAGAAGTACCTCTTTGTGATTAAGGAAATCCAAAAGCAATCTTCGATGTGA", "aa": "MNNQSNHFTNPLLQLIKNVAETKNLAIDDVVLCLKTALAQTYKKHLNYVNVEVNIDFNKGLMQIEQLFDVVDDNNEDYDDFLEMPLSEAKKLNPNLEVGGVLRKPVSLKDIKGDLISKMVLLFNQKINETAFKTVMSDFINEVGQVIEARVEDIDTNKDGGLKGYIVNLETTKGYMPKRELSKGEKLDIGKKYLFVIKEIQKQSSM", "aa_hexdigest": "e653730c22b437c5b52a87db46e5c296", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08529.16", "name": "NusA_N", "length": 120, "aa_cov": 0.5776699029126213, "hmm_cov": 0.9583333333333334, "evalue": 5.521621538342597e-20, "score": 67.26580810546875, "start": 12, "stop": 130 } ], "seq_stats": { "molecular_weight": 23415.737200000007, "isoelectric_point": 5.359200859069825 }, "id": "APKECPOBEN_397", "locus": "GCA002090215_00361" }, { "type": "cds", "sequence": "contig_1", "start": 206438, "stop": 206644, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13184.12" ], "nt": "TTGCTAAATTCCAACACTCCCGAAATTGCTAACGGTACGATCGAAATTAAAAAGATGGAACGCTCTCCTGGTACCAAGTCTAAGGTGGCCGTGATTTCCAAAGACCCAGTAGTTGATCCGATTGCGGCCATTTTGGGTCCCAAGGGCGAGCGCATTCGCGGCATTAGTGAAGAGTTTAACGGTGAGATCATTGATATTGTCATCTGA", "aa": "MLNSNTPEIANGTIEIKKMERSPGTKSKVAVISKDPVVDPIAAILGPKGERIRGISEEFNGEIIDIVI", "aa_hexdigest": "3cc5c7e3d8110d3a85095e519a519cb2", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13184.12", "name": "KH_5", "length": 45, "aa_cov": 0.6617647058823529, "hmm_cov": 1.0, "evalue": 2.0775887853446346e-13, "score": 45.73484802246094, "start": 23, "stop": 67 } ], "seq_stats": { "molecular_weight": 7301.397599999999, "isoelectric_point": 5.311058235168456 }, "id": "APKECPOBEN_398", "locus": "GCA002090215_00362" }, { "type": "cds", "sequence": "contig_1", "start": 206666, "stop": 207397, "strand": "+", "frame": 2, "gene": "nusA", "product": "Transcription termination/antitermination protein NusA", "db_xrefs": [ "GO:0003700", "GO:0003723", "GO:0005829", "GO:0006353", "GO:0031564", "SO:0001217", "UniRef:UniRef50_P47387", "UniRef:UniRef90_P75591" ], "nt": "TTGGTCAACGCTGTTTTACCGGCTGAAGTGGTCGGTTACAACATCCTTCAAGACGATGAGCGCGATACCAGCATTGAAATCGTGGTACCCGCCAACCAGATTGCCAACGTCTTTGGTTTTAAAGGAATTAACATTCGCTTAATTAGTAACCTAACGGGTTGGAGTAGTGTGGACGTTTACACCGAAAAAGATGCTGCTGAACAAGGGATTGAATTCACCCGGGTCAACTTTCAACCCCAAGGTATTTTTGGCATCAAAAAGCGCCGCGACAAGATCTCCAACAATCCACGCAACAACAACCAACAGCTCGCATCTGATAAGGTGTTTTACACTTCGAAGGCCAATGTGGTTGATGATGAGATCATAGTCGATCTAGCAAAGCAAGCGGAAGCCAAACGGGTCAAGCAAATCAAGCAGGAAGCAACTAAACCCGAGTTGCAATTACAACAAGAACTTAACCTCGAAGCAACCCCTAAGGTAGCTGCTCCAACCCCAACCCCTGCACCCCAACCAACTCCCGCACCAACTAAAGTTGAGCCAGTACCACCACCAGTAAGCGTTACACCTAAGCCAATCCCCAAGGTCAATAAGCCCAAGCCAGTGGTTAAACCAAAATCAGTCTTTTCGATTACGGTTGAAGCGGATGACAGTAAGACTAAACCAGAAAAGTCAAGCGCTAAAACTAACACCCCACAAACGAAACAAACCTTTGATAACTTTGACGATCTCTAG", "aa": "MVNAVLPAEVVGYNILQDDERDTSIEIVVPANQIANVFGFKGINIRLISNLTGWSSVDVYTEKDAAEQGIEFTRVNFQPQGIFGIKKRRDKISNNPRNNNQQLASDKVFYTSKANVVDDEIIVDLAKQAEAKRVKQIKQEATKPELQLQQELNLEATPKVAAPTPTPAPQPTPAPTKVEPVPPPVSVTPKPIPKVNKPKPVVKPKSVFSITVEADDSKTKPEKSSAKTNTPQTKQTFDNFDDL", "aa_hexdigest": "791ab3cc43f97d94518289cced24f802", "start_type": "TTG", "rbs_motif": null, "genes": [ "nusA" ], "truncated": "5-prime", "pseudogene": { "start": 206366, "stop": 207397, "observations": { "mutation": [ 206393, 206642 ], "stop": [ 206393, 206642 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75591", "query_cov": 1.0, "subject_cov": 0.45, "identity": 0.996, "score": 375.0, "evalue": 1.41e-125, "gene_start": 1, "gene_end": 243, "reference_start": 298, "reference_end": 540, "reference_sequence": "MNNQSNHFTNPLLQLIKNVAETKNLAIDDVVLCLKTALAQTYKKHLNYVNVEVNIDFNKGLMQIEQLFDVVDDNNEDYDDFLEMPLSEAKKLNPNLEVGGVLRKPVSLKDIKGDLISKMVLLFNQKINETAFKTVMSDFINEVGQVIEARVEDIDTNKDGGLKGYIVNLETTKGYMPKRELSKGEKLDIGKKYLFVIKEIQKQSSMWPITLSRSDSRLLEFLLNSNTPEIANGTIEIKKMERSPGTKSKVAVISKDPVVDPIAAILGPKGERIRGISEEFNGEIIDIVIWNEDKLKFLVNAVLPAEVVGYNILQDDERDTSIEIVVPANQIANVFGFKGINIRLISNLTGWSSVDVYTEKDAAEQGIEFTRVNFQPQGIFGIKKRRDKISNNPRNNNQQLASDKVFYTSKANVVDDEIIVDLAKQAEAKRVKQIKQEATKPELQLQQELNLEATPKVAAPTPTPAPQPTPAPTKVEPVPPPVSVTPKPIPKVNKPKPVVKPKSVFSITVEADDSKTKPEKSSAKTNTPQTKQTFDNFDDL" }, "paralog": false, "identity": 0.9941690962099126, "score": 577.0, "evalue": 3.35e-207, "description": "Internal stop codon at 206393, 206642. Nonsense mutation around 206393, 206642.", "psc": { "uniref90_id": "UniRef90_P75591", "gene": "nusA", "product": "Transcription termination/antitermination protein NusA", "uniref50_id": "UniRef50_P47387", "go_ids": [ "GO:0005829", "GO:0003700", "GO:0003723", "GO:0006353", "GO:0031564" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75591", "UniRef:UniRef50_P47387", "GO:0005829", "GO:0003700", "GO:0003723", "GO:0006353", "GO:0031564" ] }, "pscc": { "uniref50_id": "UniRef50_P47387", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47387" ], "product": "Transcription termination/antitermination protein NusA" } }, "id": "APKECPOBEN_399", "locus": "GCA002090215_00363" }, { "type": "cds", "sequence": "contig_1", "start": 207422, "stop": 207691, "strand": "+", "frame": 2, "gene": "ylxR", "product": "Transcription termination protein", "db_xrefs": [ "COG:AJ", "COG:COG2740", "KEGG:K07742", "RefSeq:WP_015344887.1", "SO:0001217", "UniParc:UPI0002B84478", "UniRef:UniRef100_A0AB38W8E2", "UniRef:UniRef50_A0AB38W8E2", "UniRef:UniRef90_A0AB38W8E2" ], "nt": "ATGCAAAGTTGGCGCCAATGTTTAGTTACCAGAAAGTCTTTTGCTAAGCCGCTACTTTTGCGTCTTGTAAAATTAGAGCATCAACTACGGGTGGATTTAGCGCAAACACTACCAGGTCGGGGGTATTATTTAAGTGTAGCTGCTTTAAAACTGGAGCGGAAAAACTTTAAAGCACTGTTACAAAAACGCTTAAAGGTAAGCTGTTCTGACGCAGAGCTAGATCACATTATTGCTTGTTTAAAGGAAAGGGAAAGCGATGTCAAAGCATAA", "aa": "MQSWRQCLVTRKSFAKPLLLRLVKLEHQLRVDLAQTLPGRGYYLSVAALKLERKNFKALLQKRLKVSCSDAELDHIIACLKERESDVKA", "aa_hexdigest": "4776d9d969246ce69f2cdc0f210ccdfa", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0002B84478", "ncbi_nrp_id": "WP_015344887.1", "uniref100_id": "UniRef100_A0AB38W8E2", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002B84478", "RefSeq:WP_015344887.1", "UniRef:UniRef100_A0AB38W8E2" ] }, "ips": { "uniref100_id": "UniRef100_A0AB38W8E2", "uniref90_id": "UniRef90_A0AB38W8E2" }, "psc": { "uniref90_id": "UniRef90_A0AB38W8E2", "gene": "YlxR", "product": "Transcription termination protein", "uniref50_id": "UniRef50_A0AB38W8E2", "cog_id": "COG2740", "cog_category": "AJ", "kegg_orthology_id": "K07742" }, "pscc": { "uniref50_id": "UniRef50_A0AB38W8E2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W8E2" ], "product": "Transcription termination protein" }, "genes": [ "ylxR" ], "id": "APKECPOBEN_400", "locus": "GCA002090215_00364" }, { "type": "cds", "sequence": "contig_1", "start": 207678, "stop": 209531, "strand": "+", "frame": 3, "gene": "infB", "product": "translation initiation factor IF-2", "db_xrefs": [ "COG:COG0532", "COG:J", "GO:0003743", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0005829", "GO:0006413", "KEGG:K02519", "RefSeq:WP_010874512.1", "SO:0001217", "UniParc:UPI000012D2E1", "UniRef:UniRef100_P75590", "UniRef:UniRef50_P47388", "UniRef:UniRef90_P75590" ], "nt": "ATGTCAAAGCATAAACCACGTCATTTTCAAAAGAATAAGTTTGACAACCGGGCTAAAACGAGTGCCAAACAACAGTTTCGCCAGGTCAAAACCGGTGTTAAAGATGGTGTCTTTGTCTATAAGGGACCACTCACTGTTAGTGAGTTTTGTCTCAAAACCAATATTCCGACGGCCAACATCATCAAGCACTTCTTTTTAAATGGGGTTCCCTTAACCTTAAATTCGGTGTTATCGACCGAACAACTAGCTGACGCTTGTGTTAACTTTGGCTTTGACTTTAAGGTAGAAACCGAAATCACCCACGACAACATTATCTCCAACATTAAGTTTGACGATGATCCCACCCAGTTAAGTCCAAGACCCCCGATCGTTACCATTATGGGTCACGTGGATCACGGGAAAACATCCTTGTTAGACGCCATTCGCCAAACTAATACAGCAGCCAAAGAGTTTGGTGGCATTACCCAAAAGATTGGGGCGTACCAGGTCAAAAACCAGGAGGGCAAAACGATTACCTTCATTGATACCCCCGGTCATGAAGCCTTTACCGGCATGCGCGCCCGTGGTGCTCAAGTGACTGACATTGTTGTCTTAGTGGTAGCGGGTGATGATGGCTTAAAACAACAAACTGAGGAAGCGATTTCCCACGCCAAGAGTGCCAAAACCCCGATCATTGTCTTTATTAACAAGATGGATAAACCAACCGCTAACCCCGACATGGTGATCCAACAGCTCAACAAGTTTGATTTAGTCCCTGAAGAATGGGGTGGTGATACCATCTTTGTCAAGGGCAGTGCCTTAACCAAAGAGGGGATCCAGGAGCTCTTGGACAGTATTCTGTTAGTAGCGGAAGTGGAAGATTACAAGGCCAACTTTAACGCCCACTCATCGGGTTATGCGATTGAGGTGCAAACCACCAAGGGGTTGGGTCCCACCGCTACCATTATTGTTAAACGTGGTACCCTAAAGATTGGTGACATCGTTGTCTTGGGTCCCGCTTGGGGTAAGGTTCGTACCATGCAAGACGAAAACGGAGTCCATCTCCAAGAAGCTATGCCCTCTAAACCAGTACAAATTTCGGGGTTTGACATTGTCCCAGTTGCTGGGGAAAAATTCATTGTCTTTGACGATGAAAAGGATGCGAAGTTAATTGCCAATAAGTTCCGCGAACAGCAAAAACAAAAGCTCAATACCACCCAAATTAACGAGGAGCTCAAACAAAAGATTAAGAGCAAGGAAATTAAGGTTTTAAATCTGATTTTTAAGGTTGACAGTGATGGTAGTTTAGCGGCCATTAAACAAGCTATGCAGAGCATTGATGTGCCGGGGATGAGCGTCAACATTATCCACTCAGGTGTTGGTTTAATCTCGGAAAACGACATTATGTTAGCCAAGGCGTCCGGGGCATTACTGTTTAGCTTGAACCTCGGTTTAAGTCAAGTTGTTAAGAACATTGCTAGTCTTCAAGGAGTGAAGGTCGATGTCCACTACCACATCCCTAAGTTAGCGGAAGAGATCGAAAACATCTTAAAGGGCCAGTTAGAACCAGTGTACGAAGACGTGGAACTGGGGCGTGCTGAAGTGTTACAACTCTGGTACCACTCCAAGGTGGGGCACATTGCTGGTACCTTAGTTAAAACAGGTAAGGTTAAACGGGGCGCCTTGTGTAAGCTGTTACGCCGTAACGAAACCATCTATGAAGGCCGGGTGGACTCATTGAAGAGTGAAAAGAACCCGGTCAACCAGATGGAAGCGGGGAAGAACTGCGGTATTGTTATTAATGGGTGTGAGGACATTCAGGTGTGCGACATTATCTTGGTGTACGAAAAGCAGGAGGTCAAGTCCAAAAGTTAA", "aa": "MSKHKPRHFQKNKFDNRAKTSAKQQFRQVKTGVKDGVFVYKGPLTVSEFCLKTNIPTANIIKHFFLNGVPLTLNSVLSTEQLADACVNFGFDFKVETEITHDNIISNIKFDDDPTQLSPRPPIVTIMGHVDHGKTSLLDAIRQTNTAAKEFGGITQKIGAYQVKNQEGKTITFIDTPGHEAFTGMRARGAQVTDIVVLVVAGDDGLKQQTEEAISHAKSAKTPIIVFINKMDKPTANPDMVIQQLNKFDLVPEEWGGDTIFVKGSALTKEGIQELLDSILLVAEVEDYKANFNAHSSGYAIEVQTTKGLGPTATIIVKRGTLKIGDIVVLGPAWGKVRTMQDENGVHLQEAMPSKPVQISGFDIVPVAGEKFIVFDDEKDAKLIANKFREQQKQKLNTTQINEELKQKIKSKEIKVLNLIFKVDSDGSLAAIKQAMQSIDVPGMSVNIIHSGVGLISENDIMLAKASGALLFSLNLGLSQVVKNIASLQGVKVDVHYHIPKLAEEIENILKGQLEPVYEDVELGRAEVLQLWYHSKVGHIAGTLVKTGKVKRGALCKLLRRNETIYEGRVDSLKSEKNPVNQMEAGKNCGIVINGCEDIQVCDIILVYEKQEVKSKS", "aa_hexdigest": "e37fbe9c658540fae8807ad3291d2eae", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000012D2E1", "ncbi_nrp_id": "WP_010874512.1", "uniref100_id": "UniRef100_P75590", "db_xrefs": [ "SO:0001217", "UniParc:UPI000012D2E1", "RefSeq:WP_010874512.1", "UniRef:UniRef100_P75590" ] }, "ips": { "uniref100_id": "UniRef100_P75590", "uniref90_id": "UniRef90_P75590" }, "psc": { "uniref90_id": "UniRef90_P75590", "gene": "infB", "product": "translation initiation factor IF-2", "uniref50_id": "UniRef50_P47388", "cog_id": "COG0532", "cog_category": "J", "kegg_orthology_id": "K02519", "go_ids": [ "GO:0003743", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0005829", "GO:0006413" ] }, "pscc": { "uniref50_id": "UniRef50_P47388", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47388" ], "product": "Translation initiation factor IF-2" }, "genes": [ "infB" ], "id": "APKECPOBEN_401", "locus": "GCA002090215_00365" }, { "type": "cds", "sequence": "contig_1", "start": 209534, "stop": 209884, "strand": "+", "frame": 2, "gene": "rbfA", "product": "30S ribosome-binding factor RbfA", "db_xrefs": [ "COG:COG0858", "COG:J", "GO:0005829", "GO:0006364", "GO:0030490", "GO:0043024", "KEGG:K02834", "RefSeq:WP_010874513.1", "SO:0001217", "UniParc:UPI00001331B1", "UniRef:UniRef100_P75589", "UniRef:UniRef50_P47389", "UniRef:UniRef90_P75589" ], "nt": "ATGGCTAGTTACAAAAAGGAACGTCTGGAAAACGACATTATTCGTTTAATTAACCGCACTGTTATTCACGAAATCTACAACGAAACGGTCAAAACCGGTCATGTAACCCACGTGAAACTATCGGATGATTTACTCCATGTAACAGTTTACCTAGACTGTTATAACCGTGAGCAAATTGACCGCGTGGTGGGTGCCTTTAACCAGGCCAAAGGGGTGTTTAGCCGCGTGTTAGCACACAATTTGTACTTAGCCAAAGCCGTGCAAATCCATTTTGTCAAGGACAAAGCAATTGATAACGCAATGCGCATTGAGTCTATCATCAACTCCCTTAAAAAATCAAAGCCTAATTAA", "aa": "MASYKKERLENDIIRLINRTVIHEIYNETVKTGHVTHVKLSDDLLHVTVYLDCYNREQIDRVVGAFNQAKGVFSRVLAHNLYLAKAVQIHFVKDKAIDNAMRIESIINSLKKSKPN", "aa_hexdigest": "ea805a36f7a0eafb24a3f0e2c3411375", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001331B1", "ncbi_nrp_id": "WP_010874513.1", "uniref100_id": "UniRef100_P75589", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001331B1", "RefSeq:WP_010874513.1", "UniRef:UniRef100_P75589" ] }, "ips": { "uniref100_id": "UniRef100_P75589", "uniref90_id": "UniRef90_P75589" }, "psc": { "uniref90_id": "UniRef90_P75589", "gene": "rbfA", "product": "30S ribosome-binding factor RbfA", "uniref50_id": "UniRef50_P47389", "cog_id": "COG0858", "cog_category": "J", "kegg_orthology_id": "K02834", "go_ids": [ "GO:0005829", "GO:0006364", "GO:0030490", "GO:0043024" ] }, "pscc": { "uniref50_id": "UniRef50_P47389", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47389" ], "product": "Ribosome-binding factor A" }, "genes": [ "rbfA" ], "id": "APKECPOBEN_402", "locus": "GCA002090215_00366" }, { "type": "cds", "sequence": "contig_1", "start": 209900, "stop": 210745, "strand": "+", "frame": 2, "gene": null, "product": "putative MG144-like protein", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P75588", "UniRef:UniRef90_P75588" ], "nt": "ATGAAACCACCTAAAAAGCCAGTCAACCCACCGTTTGGTAAGCTAACTGATTCTAAAGCTGGAGTAGTCAAAGCTACCTCTAGTCAAGAGACCAAAAAAGTAGCTGACACCAAACCCAAAAATAAGGGTGGTTTGTTTAGCTTCTTTAAAAAAGACAAAACGGAAAAACCAGCTAAAGCAGCCAAACCCAAAGATGCCTTTAAGAGTGCGATAGCTGAATTGAATCCGAAAGCCAATCCTAAGACAGTCAAAGCGGATGTTGCACCGGCTAAAATTCCCCACAGCGATAAGGGTACGGTTACCCCTGTAGAGTTAAAACCCCAAACGGAAGCACCGTTACCACCAGGAGTTAAACAACCTGATCCTAAAAAAGATAAACCTAAGGGCGGTTTATTTGGCTTCTTCAAAAAAGATAAGAACAAAGATGTCAAAAAGGAGCCTGCTAAGCCAGCTACTCCAGTAAAAACTGAACCAACCTCACCTAAGGTAGAACCAACTAAGGTCAAACCACCGGGCGGTGTACCGACTAAGCCGGTAGTGGAAAAACCAGTGTCAGCTCAACCAACGGTACCATTACAACCAGAACCTACTTTCCCAGTCAAAGCAGCACCACTACCACCAGGTGTAAAAGCAGAACCGGAAAGCAAAAAACGCTTTGGTTTGTTTAAGGCATTCCAGAAAGATGACGCTAAACAACCCAAGCAAAAGCTCAATCTCCAAGACCAACAGGATCGCTTTATTGATGACCCCACCGCCAAAAAGCACTTTAGCGCCTTTAACCAAAAAGTTGGTAACTTACTGAAGGACAAAAAGACCCGTAACCGTGATTGGAAGATTGTGGGTTGA", "aa": "MKPPKKPVNPPFGKLTDSKAGVVKATSSQETKKVADTKPKNKGGLFSFFKKDKTEKPAKAAKPKDAFKSAIAELNPKANPKTVKADVAPAKIPHSDKGTVTPVELKPQTEAPLPPGVKQPDPKKDKPKGGLFGFFKKDKNKDVKKEPAKPATPVKTEPTSPKVEPTKVKPPGGVPTKPVVEKPVSAQPTVPLQPEPTFPVKAAPLPPGVKAEPESKKRFGLFKAFQKDDAKQPKQKLNLQDQQDRFIDDPTAKKHFSAFNQKVGNLLKDKKTRNRDWKIVG", "aa_hexdigest": "92342a83eaeca0e6f20fa17fcc07da2c", "start_type": "ATG", "rbs_motif": "AAAA", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 209900, "stop": 211048, "observations": { "mutation": [ 210743 ], "stop": [ 210743 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75588", "query_cov": 1.0, "subject_cov": 0.6990049751243781, "identity": 1.0, "score": 407.0, "evalue": 2.77e-139, "gene_start": 1, "gene_end": 281, "reference_start": 1, "reference_end": 281, "reference_sequence": "MKPPKKPVNPPFGKLTDSKAGVVKATSSQETKKVADTKPKNKGGLFSFFKKDKTEKPAKAAKPKDAFKSAIAELNPKANPKTVKADVAPAKIPHSDKGTVTPVELKPQTEAPLPPGVKQPDPKKDKPKGGLFGFFKKDKNKDVKKEPAKPATPVKTEPTSPKVEPTKVKPPGGVPTKPVVEKPVSAQPTVPLQPEPTFPVKAAPLPPGVKAEPESKKRFGLFKAFQKDDAKQPKQKLNLQDQQDRFIDDPTAKKHFSAFNQKVGNLLKDKKTRNRDWKIVGWIHGLILLFFIPLLAIMNKFVTLPAQSYPAVSLQVSINNALWGIAIFVIANIALPFITMFVLFLTGVRDVHASRPVHYFIWVLMLLNLTFLVISCCLLAAAYANLDLYNIWRNLQALDPNN" }, "paralog": false, "identity": 0.9973821989528796, "score": 625.0, "evalue": 1.03e-227, "description": "Internal stop codon at 210743. Nonsense mutation around 210743.", "psc": { "uniref90_id": "UniRef90_P75588", "product": "Uncharacterized protein MG144 homolog", "uniref50_id": "UniRef50_P75588", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75588", "UniRef:UniRef50_P75588", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75588", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75588" ], "product": "Uncharacterized protein MG144 homolog" } }, "id": "APKECPOBEN_403", "locus": "GCA002090215_00367" }, { "type": "cds", "sequence": "contig_1", "start": 210791, "stop": 211075, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAAATTTGTGACCCTCCCAGCACAAAGCTATCCTGCTGTATCACTACAAGTATCGATTAACAATGCGTTGTGGGGGATTGCGATCTTTGTGATAGCCAACATTGCTCTACCCTTCATTACGATGTTCGTGCTCTTCTTGACTGGGGTCAGAGATGTGCATGCTAGTCGGCCAGTGCACTATTTCATCTGGGTGTTAATGTTGTTAAACCTTACCTTCTTAGTAATTAGTTGTTGTTTACTGGCTGCTGCTTATGCTAACTTAGACCTTTACAACATCTGA", "aa": "MNKFVTLPAQSYPAVSLQVSINNALWGIAIFVIANIALPFITMFVLFLTGVRDVHASRPVHYFIWVLMLLNLTFLVISCCLLAAAYANLDLYNI", "aa_hexdigest": "8abd6db1127ed8df2c152ac3a05b85c9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10504.50679999999, "isoelectric_point": 7.825124168395997 }, "id": "APKECPOBEN_404", "locus": "GCA002090215_00368" }, { "type": "cds", "sequence": "contig_1", "start": 211121, "stop": 211624, "strand": "+", "frame": 2, "gene": "ribF", "product": "riboflavin biosynthesis protein RibF", "db_xrefs": [ "COG:COG0196", "COG:H", "EC:2.7.1.26", "EC:2.7.7.2", "GO:0003919", "GO:0005524", "GO:0005737", "GO:0006747", "GO:0008531", "GO:0009231", "GO:0009398", "KEGG:K11753", "SO:0001217", "UniRef:UniRef50_P75587", "UniRef:UniRef90_P75587" ], "nt": "ATGCAGCAAACCTTGATCATCGGTGCTTTTGATGGTTTACACAAGGGTCATCAATTGTTAGCACAAGCGGCTAGTGGTCCAGTGGTGGCCTTGTTAATTGCTAACATTCCCAGTCTACAAAGTGACTGGCTATACGAACCGAAGCAACGGCAAGTGCAGTTACAACAACACTTTCAGTCCGTGGTGCACAGCTATGATGTGATTGAACACAACATTAGTGCCCAAGCGTTTTTCGATCAAATCATTAGCCCACTACGGTGTCAGCAATTAGTCGTGGGTGCGGACTTTTGCTTTGGTAAAGACAACCAGAATGCTGATTTTTTAAGACGTTTATTTCCCAATACCACGATTATTCCCAAAGACAGTCAAACGCTGTCCAGTTCTACCATTCGCCAATGGTTAAAGCAGGGCCAGATAGAACAAGCCAACGCCGTGTTATTGGAACCCTACTTTAGGGAAGGGGTGGTTATTCGTGGTAACCAGCAAGCCCGGTTTTTGGGCTGA", "aa": "MQQTLIIGAFDGLHKGHQLLAQAASGPVVALLIANIPSLQSDWLYEPKQRQVQLQQHFQSVVHSYDVIEHNISAQAFFDQIISPLRCQQLVVGADFCFGKDNQNADFLRRLFPNTTIIPKDSQTLSSSTIRQWLKQGQIEQANAVLLEPYFREGVVIRGNQQARFLG", "aa_hexdigest": "3f984b340a4f108a656631047a50588b", "start_type": "ATG", "rbs_motif": null, "genes": [ "ribF" ], "truncated": "3-prime", "pseudogene": { "start": 211121, "stop": 211927, "observations": { "mutation": [ 211622 ], "stop": [ 211622 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75587", "query_cov": 1.0, "subject_cov": 0.620817843866171, "identity": 1.0, "score": 332.0, "evalue": 6.87e-114, "gene_start": 1, "gene_end": 167, "reference_start": 1, "reference_end": 167, "reference_sequence": "MQQTLIIGAFDGLHKGHQLLAQAASGPVVALLIANIPSLQSDWLYEPKQRQVQLQQHFQSVVHSYDVIEHNISAQAFFDQIISPLRCQQLVVGADFCFGKDNQNADFLRRLFPNTTIIPKDSQTLSSSTIRQWLKQGQIEQANAVLLEPYFREGVVIRGNQQARFLGWPTANITLKPYMVPLRCGSYVITVTYHQTNYPGVGFISYKNDQLVCETHLIGFSGDLYGKQLRFTFNQFIRPQQKFSGVQALQKAISGDLKQAQKWFAQSTN" }, "paralog": false, "identity": 0.996268656716418, "score": 539.0, "evalue": 1.18e-197, "description": "Internal stop codon at 211622. Nonsense mutation around 211622.", "psc": { "uniref90_id": "UniRef90_P75587", "gene": "ribF", "product": "riboflavin biosynthesis protein RibF", "ec_ids": [ "2.7.1.26", "2.7.7.2" ], "uniref50_id": "UniRef50_P75587", "cog_id": "COG0196", "cog_category": "H", "kegg_orthology_id": "K11753", "go_ids": [ "GO:0003919", "GO:0005524", "GO:0005737", "GO:0006747", "GO:0008531", "GO:0009231", "GO:0009398" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75587", "EC:2.7.1.26", "EC:2.7.7.2", "UniRef:UniRef50_P75587", "COG:COG0196", "COG:H", "KEGG:K11753", "GO:0003919", "GO:0005524", "GO:0005737", "GO:0006747", "GO:0008531", "GO:0009231", "GO:0009398" ] }, "pscc": { "uniref50_id": "UniRef50_P75587", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75587" ], "product": "Bifunctional riboflavin kinase/FMN adenylyltransferase" } }, "id": "APKECPOBEN_405", "locus": "GCA002090215_00369" }, { "type": "cds", "sequence": "contig_1", "start": 211655, "stop": 211930, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01687.23" ], "nt": "ATGGTGCCGTTGCGCTGTGGGAGCTATGTGATTACGGTGACTTACCACCAAACCAATTACCCGGGTGTAGGGTTTATCAGTTACAAAAACGACCAGTTAGTCTGTGAAACCCACTTAATTGGTTTTAGTGGTGATCTTTACGGTAAACAACTACGCTTTACCTTCAACCAGTTTATTCGCCCCCAGCAAAAGTTTAGTGGGGTGCAAGCGTTGCAAAAAGCGATTAGTGGTGATTTAAAGCAAGCGCAGAAGTGGTTTGCTCAATCAACAAATTAA", "aa": "MVPLRCGSYVITVTYHQTNYPGVGFISYKNDQLVCETHLIGFSGDLYGKQLRFTFNQFIRPQQKFSGVQALQKAISGDLKQAQKWFAQSTN", "aa_hexdigest": "39d300f6a3c9eeaae46e8384c823eb85", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01687.23", "name": "Flavokinase", "length": 87, "aa_cov": 0.9010989010989011, "hmm_cov": 1.0, "evalue": 1.119526072016403e-19, "score": 66.33644104003906, "start": 5, "stop": 86 } ], "seq_stats": { "molecular_weight": 10353.698899999992, "isoelectric_point": 9.469003486633301 }, "id": "APKECPOBEN_406", "locus": "GCA002090215_00370" }, { "type": "cds", "sequence": "contig_1", "start": 211956, "stop": 212405, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01595.25" ], "nt": "ATGGAAAGTGCCCCCAGTGGTGGCCTGTTGGCCTTAATTATTATCAGTATTATCCTCCTGGCTTGTATTAGTGCCGTGGTTTCGGCTTACGAAACCGCCATTACCTCAATTACATCATACAAGTGGAGCAACTATGTCAAAACGCATAACAAGCAAAAGAAGTTAACGACCAAGATTGTTAACCACTTCCAAAAGAACTATTCGGCTTGTTTAATTACCATCTTAGTGGCCAATAACATTGTCGCCATTTTGGTTTCCAACATCCTCTTTTTGGCTTTAGATCAATCGATTAAGAATCCGGCCATTTCCAGTGCCTTGAACCTCTTAATTAGTGGGGTGTTATTGTTAATGCTGTGTGAAATTACTCCCAAAACCTTAGCACGGATTAACATCATCCGCGTCTTGGTCTACTTTGCGGTGGTAGTGTATTTTTTCTACATCCTTTTTTGA", "aa": "MESAPSGGLLALIIISIILLACISAVVSAYETAITSITSYKWSNYVKTHNKQKKLTTKIVNHFQKNYSACLITILVANNIVAILVSNILFLALDQSIKNPAISSALNLLISGVLLLMLCEITPKTLARINIIRVLVYFAVVVYFFYILF", "aa_hexdigest": "ad7724b1de6b65499b3fe5f323baff98", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01595.25", "name": "CNNM", "length": 135, "aa_cov": 0.9060402684563759, "hmm_cov": 0.9629629629629629, "evalue": 1.5083527130889852e-20, "score": 69.0667495727539, "start": 15, "stop": 149 } ], "seq_stats": { "molecular_weight": 16423.589599999985, "isoelectric_point": 9.484218025207518 }, "id": "APKECPOBEN_407", "locus": "GCA002090215_00371" }, { "type": "cds", "sequence": "contig_1", "start": 212601, "stop": 213230, "strand": "+", "frame": 3, "gene": "mpfA", "product": "UPF0053 MG146-like protein", "db_xrefs": [ "COG:COG1253", "COG:PT", "GO:0005886", "SO:0001217", "UniRef:UniRef50_Q49399", "UniRef:UniRef90_P75586" ], "nt": "GTGGTGTACTGTTATGAAGGTGATCCAGTCAAGACCATTAAGGAAAAGTTCTTGCACGGCCAGTTTTCCAGAATGCCCGTACTCGACCAGACAAGCAATGAAGTGGTTGGTTTTATCCACCTCAAGGACTTGTTTAGTTCACTCGAAAAGAGCAACGAGCCGTTTGTTTTACAGGAGTTATTGTATCCAGCTGTTTTAGTAAGTAACACCACACCCATCAAGCAAGCTTTGCGCCAAATGCGGTTACACCGGGCCCACTTAGCGGTCGTGCAGGACAAACACCACCACACGATCGGGATTGTTTCTATGGAAGACATCATTGAGGAATTAGTCGGGGAAATTTACGATGAACATGATGAGGTGGAGGCAGTGCAAACGTTGGACAACAACACCTGGTTAGTGTTACCGAACGTAAAAGCCGCCTTTTTCTTTAACAAGTGGATTAAACGGGATTTAGTAAAATCAAAAAACATGACGATCCAACGTTACTTGGCATCGTTTGAAAATGATGGGTTAAACACCCAGAATAAACTAGAAACGCCGTGGTTTATTGCTGAAGCAATTGTCGACAGCGAGAACCCCGAGCAAATTCGGTACGAAATTCGCAAAAAGAGTGATGTTGTGGACTAG", "aa": "MVYCYEGDPVKTIKEKFLHGQFSRMPVLDQTSNEVVGFIHLKDLFSSLEKSNEPFVLQELLYPAVLVSNTTPIKQALRQMRLHRAHLAVVQDKHHHTIGIVSMEDIIEELVGEIYDEHDEVEAVQTLDNNTWLVLPNVKAAFFFNKWIKRDLVKSKNMTIQRYLASFENDGLNTQNKLETPWFIAEAIVDSENPEQIRYEIRKKSDVVD", "aa_hexdigest": "1382fc4d7ae0a4de2be93c9c47d66841", "start_type": "GTG", "rbs_motif": null, "genes": [ "mpfA" ], "truncated": "5-prime", "pseudogene": { "start": 212301, "stop": 213230, "observations": { "mutation": [ 212403, 212592 ], "stop": [ 212403, 212592 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75586", "query_cov": 1.0, "subject_cov": 0.49061032863849763, "identity": 0.995, "score": 414.0, "evalue": 6.5e-143, "gene_start": 1, "gene_end": 209, "reference_start": 218, "reference_end": 426, "reference_sequence": "MTYGKCPSGGLLALIIISIILLACISAVVSAYETAITSITSYKWSNYVKTHNKQKKLTTKIVNHFQKNYSACLITILVANNIVAILVSNILFLALDQSIKNPAISSALNLLISGVLLLMLCEITPKTLARINIIRVLVYFAVVVYFFYILFWPITKLASIIFAKYEKAPPVSRRDVYFFIDEIEQNGLFTKEDGQLIKRTLIFDQVLVDQIMIKWNRVVYCYEGDPVKTIKEKFLHGQFSRMPVLDQTSNEVVGFIHLKDLFSSLEKSNEPFVLQELLYPAVLVSNTTPIKQALRQMRLHRAHLAVVQDKHHHTIGIVSMEDIIEELVGEIYDEHDEVEAVQTLDNNTWLVLPNVKAAFFFNKWIKRDLVKSKNMTIQRYLASFENDGLNTQNKLETPWFIAEAIVDSENPEQIRYEIRKKSDVVD" }, "paralog": false, "identity": 0.9935275080906149, "score": 608.0, "evalue": 1.08e-221, "description": "Internal stop codon at 212403, 212592. Nonsense mutation around 212403, 212592.", "psc": { "uniref90_id": "UniRef90_P75586", "gene": "MpfA", "product": "UPF0053 protein MG146 homolog", "uniref50_id": "UniRef50_Q49399", "cog_id": "COG1253", "cog_category": "PT", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75586", "UniRef:UniRef50_Q49399", "COG:COG1253", "COG:PT", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_Q49399", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49399" ], "product": "UPF0053 protein MG146" } }, "id": "APKECPOBEN_408", "locus": "GCA002090215_00372" }, { "type": "cds", "sequence": "contig_1", "start": 213217, "stop": 213954, "strand": "+", "frame": 1, "gene": null, "product": "MPN160 family protein", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P47393", "UniRef:UniRef90_P75585" ], "nt": "ATGTTGTGGACTAGAGCCTTAATCTTAGAGTTAAAAACCAATAAGCAAAGCCGCTTGTTGTGGTTACTTGCAATTCCCTTACTAATATCGTTAACCTTACTAACGTACGGTTTAGTGTTGTTTAGTTCCAGTGGTCGAATTGATCATGGTGATCACTTCCACTTACGTGAGCGCTTTGTGTTAACGACCGAAGAGTTAGTTACCTTTGTGGTAGCGAGTGTCGTGTTTGCGCTAACTGTTGCCTTGTTTGGGCTGGGTTGCTGGAAGTTGCTCCAAGGTCCAAAAGTGGATAGAACAAACATAAAGCTAGCAAACAGCAATCCTGCTCCCCAAGCCGTGGTATTGCAAGCTGATTGTGATCACTTTCAGGTAGGGGATCACTGTGTGTTTAGCGCTGAAAAGCAACACTTTAAACAACAGTTCAAACAGGACTTTTTGGGTAAAAGTAAGTTTTCCTTTCGCAATGAACTGTACCGTTTTTGTCTCATTGGTGTGTTAATTTCGCTGAATTTAGCGCTCAGTATGGTGGAAATCCCCGGCATTGTTCTACCGTGGGGCAGTTCAATCCAGTTCCGCTTTTTTAACACCGCCATCTTGTTTATTGCGATTCGCTTTGTTGGGTTACTCTCCACATCGTTAATTGCGATTATTACTCCATGGATTCACCTCCTCCTTCACCCCGTTCATACCCCGATTAGTACCGTTTTTTACATGGGCAATGACTTGGTAGTTCTGTGA", "aa": "MLWTRALILELKTNKQSRLLWLLAIPLLISLTLLTYGLVLFSSSGRIDHGDHFHLRERFVLTTEELVTFVVASVVFALTVALFGLGCWKLLQGPKVDRTNIKLANSNPAPQAVVLQADCDHFQVGDHCVFSAEKQHFKQQFKQDFLGKSKFSFRNELYRFCLIGVLISLNLALSMVEIPGIVLPWGSSIQFRFFNTAILFIAIRFVGLLSTSLIAIITPWIHLLLHPVHTPISTVFYMGNDLVVL", "aa_hexdigest": "51ce5f08758fd1099edbd881ce19b60a", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 213217, "stop": 214257, "observations": { "mutation": [ 213952 ], "stop": [ 213952 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75585", "query_cov": 1.0, "subject_cov": 0.649867374005305, "identity": 1.0, "score": 471.0, "evalue": 1.61e-165, "gene_start": 1, "gene_end": 245, "reference_start": 1, "reference_end": 245, "reference_sequence": "MLWTRALILELKTNKQSRLLWLLAIPLLISLTLLTYGLVLFSSSGRIDHGDHFHLRERFVLTTEELVTFVVASVVFALTVALFGLGCWKLLQGPKVDRTNIKLANSNPAPQAVVLQADCDHFQVGDHCVFSAEKQHFKQQFKQDFLGKSKFSFRNELYRFCLIGVLISLNLALSMVEIPGIVLPWGSSIQFRFFNTAILFIAIRFVGLLSTSLIAIITPWIHLLLHPVHTPISTVFYMGNDLVVLWIFYFFYYHIFKAEVKQTTTVVNNKEFSQLVNTHKTKVAKALALIPVNLICGFIEGLGFYVGYFLILGKFGSVGHKIFYDSQANRDLINSANVIYFLLTTTTIFSLKYLFELLFFYSVEKGILNISRHFGLY" }, "paralog": false, "identity": 0.9971098265895953, "score": 687.0, "evalue": 3.18e-253, "description": "Internal stop codon at 213952. Nonsense mutation around 213952.", "psc": { "uniref90_id": "UniRef90_P75585", "product": "MPN160 family protein", "uniref50_id": "UniRef50_P47393", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75585", "UniRef:UniRef50_P47393", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47393", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47393" ], "product": "Uncharacterized protein MG147" } }, "id": "APKECPOBEN_409", "locus": "GCA002090215_00373" }, { "type": "cds", "sequence": "contig_1", "start": 214075, "stop": 214350, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGCGTTAATTCCAGTTAATTTAATCTGTGGTTTTATTGAGGGTTTGGGTTTCTATGTCGGTTACTTCTTGATTTTGGGTAAATTTGGTAGTGTAGGGCATAAGATTTTCTATGACAGTCAAGCTAACCGTGACCTAATTAACAGTGCTAACGTGATCTATTTCTTACTCACTACTACCACCATTTTTAGCTTAAAGTACCTGTTTGAACTACTGTTCTTTTACAGTGTCGAGAAGGGTATACTTAACATTTCGCGCCACTTTGGTTTGTATTAA", "aa": "MALIPVNLICGFIEGLGFYVGYFLILGKFGSVGHKIFYDSQANRDLINSANVIYFLLTTTTIFSLKYLFELLFFYSVEKGILNISRHFGLY", "aa_hexdigest": "11c90ec030d761a2f8b55e1f1a9cbb06", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10391.103999999994, "isoelectric_point": 7.863224983215332 }, "id": "APKECPOBEN_410", "locus": "GCA002090215_00374" }, { "type": "cds", "sequence": "contig_1", "start": 214504, "stop": 214755, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTGACGTCAAATACTTACAGGTCGAAATTGTCGATAAAAACACCATTCGCCTCCAGGAAGATGGCAAAAAGGGTCAACAAATTAGGTTAGATCAAATTATTAAGGTTGATCAAAGTAACATCTTAAACACTTTGGAGCAGGCACAACGCGAGGCATACGAAAGGGAGGCGCAAAGCCGTTACCAAACTAAGTTGGCCAAAGAACTGAGTGAAAAAGACAACACTTTTTTAAAGCAAAAAGCAGCTTGA", "aa": "MSDVKYLQVEIVDKNTIRLQEDGKKGQQIRLDQIIKVDQSNILNTLEQAQREAYEREAQSRYQTKLAKELSEKDNTFLKQKAA", "aa_hexdigest": "99d768ce69a79f5b5babefc30a898955", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9711.849399999992, "isoelectric_point": 7.926790809631349 }, "id": "APKECPOBEN_411", "locus": "GCA002090215_00375" }, { "type": "cds", "sequence": "contig_1", "start": 214936, "stop": 215082, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAATACGAACGTAAAGCAAATAAAACTAAGGAAGAAAATCAACAAGAATTAGAAAGACAACGCCAGTACTTTTTAGAACAGTTAGAACAAGCCCAAAAGGACATTGAAGAGCTCAAAACGCGTGAAGAGCGTTTTAGTAAGTGA", "aa": "MEYERKANKTKEENQQELERQRQYFLEQLEQAQKDIEELKTREERFSK", "aa_hexdigest": "d147e97b9e9c59a795c2412b96d1a0c6", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6106.661499999998, "isoelectric_point": 5.169983863830565 }, "id": "APKECPOBEN_412", "locus": "GCA002090215_00376" }, { "type": "cds", "sequence": "contig_1", "start": 215107, "stop": 215841, "strand": "+", "frame": 1, "gene": null, "product": "putative MG148-like protein", "db_xrefs": [ "COG:COG4487", "COG:S", "SO:0001217", "UniRef:UniRef50_P75584", "UniRef:UniRef90_P75584" ], "nt": "TTGGAAAAGTGGTGTTGGGAAGCTTACAAAAATTACGAAGAGTTGTTCCAAAACTGTGTGTTTGCCCCTTACAGTGAGCTAGTTAAGGGTGCCAAAAAGAGCATAGGTGTAGAAGACGATGAGAGCAATATTAACGAAAAGGCTGACTTTATCTTCCAGGTATTTAATCCCAATAATGACAAAGAACCCTTCTTTTCAATTTGCTGTGAAATGAAAACAGAGTTTACTGAAAGCAAGAGTCGCACTAAAAACGAAGATCATGTCAAAAAACTGATTGCCGACGCTAAGCGCGCTAAGTGCCAATACGGTTTTTTAGTAAGTGAATTGGAACTCAACACTGAAAACGATGTCCAAGTGCAACGGATGCATACCTCGAACAGTGAAGTGGAGGTTTACCTAGTGCGCCCAATGTTCTTCATTGTGATGTTGCGGTTGTTTTACTTTTTGGCCAAAAAGATGTTTGCCCAAGTTGATGTAAACAGCGAGTACTTAGATAAAGAAGCTTTAAATGCTAGCTTTAGCGATTTAAAGAAAAGCTTACTTGAAAAAACCTTTACTGATCTCAACAAGGTCTTTCAAAACAACATTGATGAACTAGAAAAGATTGAAGGATTAGTAACAAAGTTGAAAGCCGCTAATGAAAAGGCCTTAAATAGTCGTTTAAATAACTGGGAAGAAAAGATCCGTAAGTTTGAATTTAAGCTCAATAAAGACATTGTTAAAAAGCTTGAATAA", "aa": "MEKWCWEAYKNYEELFQNCVFAPYSELVKGAKKSIGVEDDESNINEKADFIFQVFNPNNDKEPFFSICCEMKTEFTESKSRTKNEDHVKKLIADAKRAKCQYGFLVSELELNTENDVQVQRMHTSNSEVEVYLVRPMFFIVMLRLFYFLAKKMFAQVDVNSEYLDKEALNASFSDLKKSLLEKTFTDLNKVFQNNIDELEKIEGLVTKLKAANEKALNSRLNNWEEKIRKFEFKLNKDIVKKLE", "aa_hexdigest": "0d53eec1d7093812876b189bce4ba508", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 214807, "stop": 215841, "observations": { "mutation": [ 215080 ], "stop": [ 215080 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75584", "query_cov": 1.0, "subject_cov": 0.5483146067415731, "identity": 0.996, "score": 470.0, "evalue": 3.33e-164, "gene_start": 1, "gene_end": 244, "reference_start": 202, "reference_end": 445, "reference_sequence": "MSDVKYLQVEIVDKNTIRLQEDGKKGQQIRLDQIIKVDQSNILNTLEQAQREAYEREAQSRYQTKLAKELSEKDNTFLKQKAAWNQAHNTQIQQLYQQITNLENQVNNIKRETESKKDNEYQQQIVKLETQLQSIKKETESQKDLEYERKANKTKEENQQELERQRQYFLEQLEQAQKDIEELKTREERFSKWAIAKKGKELEKWCWEAYKNYEELFQNCVFAPYSELVKGAKKSIGVEDDESNINEKADFIFQVFNPNNDKEPFFSICCEMKTEFTESKSRTKNEDHVKKLIADAKRAKCQYGFLVSELELNTENDVQVQRMHTSNSEVEVYLVRPMFFIVMLRLFYFLAKKMFAQVDVNSEYLDKEALNASFSDLKKSLLEKTFTDLNKVFQNNIDELEKIEGLVTKLKAANEKALNSRLNNWEEKIRKFEFKLNKDIVKKLE" }, "paralog": false, "identity": 0.9767441860465116, "score": 620.0, "evalue": 1.14e-225, "description": "Internal stop codon at 215080. Nonsense mutation around 215080.", "psc": { "uniref90_id": "UniRef90_P75584", "product": "Uncharacterized protein MG148 homolog", "uniref50_id": "UniRef50_P75584", "cog_id": "COG4487", "cog_category": "S", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75584", "UniRef:UniRef50_P75584", "COG:COG4487", "COG:S" ] }, "pscc": { "uniref50_id": "UniRef50_P75584", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75584" ], "product": "Uncharacterized protein MG148 homolog" } }, "id": "APKECPOBEN_413", "locus": "GCA002090215_00377" }, { "type": "cds", "sequence": "contig_1", "start": 215963, "stop": 216316, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAACTAAATCTGCGATTTCCTAGCTATTTTTTACCCGTCGTTGCCGCTAGTGCTTTCCTTGTTAGTTGCGCCACACCTAACACTTACGAAGTACAACGCGCTGCCTTGATTCAACTAGTTGAAGAAGACGAACGACAAAACTACATTCAAAAGGGTAGTATGGGTGCAAATGCTGTTATGACTGCTGCAAAAGCAGAAACCAAAACAGCAGAAAAAACAGCAACTTCTACCAAAGCTGCTTCAATAGAACTTAAAAAAACTGATACTGATATTAAGACTACAACTACTACCGAAAACAAATCAGCATCCGGATACAAACTAGATACCCTCTTTGGTGACTACATCTTGTGA", "aa": "MKLNLRFPSYFLPVVAASAFLVSCATPNTYEVQRAALIQLVEEDERQNYIQKGSMGANAVMTAAKAETKTAEKTATSTKAASIELKKTDTDIKTTTTTENKSASGYKLDTLFGDYIL", "aa_hexdigest": "a457cf17742fb2ebbb26bf4b0fb3dd76", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12752.366599999976, "isoelectric_point": 7.770879173278809 }, "id": "APKECPOBEN_414", "locus": "GCA002090215_00378" }, { "type": "cds", "sequence": "contig_1", "start": 216407, "stop": 216925, "strand": "+", "frame": 2, "gene": null, "product": "MPN162 family protein", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P75583", "UniRef:UniRef90_P75583" ], "nt": "ATGATTTTAGACGGTGGTAACTTGACCGTGGAAAAAAATCATGAACATGGCCATACTCATAAAAATGGTGAAACTCACGAACATGATCATGATCACCACGAAGGTGAAGAGGAAGTAATCGTTGGTCGTGCCCTTTCTTTTGCTAATGGTCTCTTCTTAGTTGTGGATCTTAAGGAAGAAAAACACGAAGAGAAGAAGGAAGCAAAATCTGAAATGTCAATGAACAGTAAAGATATGGTGATGATGACCAAAACTGAAATGATGTCTAAGGAAATGAAATCCGAACAAAAAATGGAGAAGAAGGAGGAACACGAACATCCTCACAAAAAGCTGAGCCTCAGTACCACGGCTTACAAGTTTGGTCAAAGCTTTAACATCCTGGAATTTACTGGTGCTATGCACCACAAAACGGCACACAATAACGAAACCGAATTTAAAAATTTAGGCAAAAAATACGGTGGTATGACCGAGTACACGATTGTCGACTTTGACTTTAATCCACCAAAACCAACTAAGTAA", "aa": "MILDGGNLTVEKNHEHGHTHKNGETHEHDHDHHEGEEEVIVGRALSFANGLFLVVDLKEEKHEEKKEAKSEMSMNSKDMVMMTKTEMMSKEMKSEQKMEKKEEHEHPHKKLSLSTTAYKFGQSFNILEFTGAMHHKTAHNNETEFKNLGKKYGGMTEYTIVDFDFNPPKPTK", "aa_hexdigest": "c129b168313a965093669a27d1f9dab8", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 216107, "stop": 216925, "observations": { "mutation": [ 216314 ], "stop": [ 216314 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75583", "query_cov": 1.0, "subject_cov": 0.5375, "identity": 1.0, "score": 275.0, "evalue": 2.91e-90, "gene_start": 1, "gene_end": 172, "reference_start": 149, "reference_end": 320, "reference_sequence": "MKLNLRFPSYFLPVVAASAFLVSCATPNTYEVQRAALIQLVEEDERQNYIQKGSMGANAVMTAAKAETKTAEKTATSTKAASIELKKTDTDIKTTTTTENKSASGYKLDTLFGDYILWVVDHLSGLLFSPKTNNSTTTQKIQLITEDKMILDGGNLTVEKNHEHGHTHKNGETHEHDHDHHEGEEEVIVGRALSFANGLFLVVDLKEEKHEEKKEAKSEMSMNSKDMVMMTKTEMMSKEMKSEQKMEKKEEHEHPHKKLSLSTTAYKFGQSFNILEFTGAMHHKTAHNNETEFKNLGKKYGGMTEYTIVDFDFNPPKPTK" }, "paralog": false, "identity": 0.9963235294117647, "score": 423.0, "evalue": 3.97e-151, "description": "Internal stop codon at 216314. Nonsense mutation around 216314.", "psc": { "uniref90_id": "UniRef90_P75583", "product": "MPN162 family protein", "uniref50_id": "UniRef50_P75583", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75583", "UniRef:UniRef50_P75583", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75583", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75583" ], "product": "Uncharacterized lipoprotein MG149 homolog" } }, "id": "APKECPOBEN_415", "locus": "GCA002090215_00379" }, { "type": "cds", "sequence": "contig_1", "start": 217124, "stop": 217510, "strand": "+", "frame": 2, "gene": null, "product": "MPN163 family protein", "db_xrefs": [ "GO:0005886", "RefSeq:WP_014574900.1", "SO:0001217", "UniParc:UPI0001DD64DA", "UniRef:UniRef100_A0A0H3DMB4", "UniRef:UniRef50_Q9ZB80", "UniRef:UniRef90_P75582" ], "nt": "TTGGAAAAAGATAAGGAATTAGTCAGTTCAATTGTGACTGCTAAAAGCATGATTGACCGGTTTTTCTGGTCTATTTTGAGCTTTCTGCTCACTAACTTAGTCTTTTTATTTGTGGCTTTTTTAATTCTGATTATTTACTTAATTTCCGAAATCACCCAACAGTTTGCCTTTGCTTTTATCGCAACGATTGTCTTCATCATTTTTTACAACATCCTCTTTTTGAGCTACCTGTTGACGATGTACATTAAAGGCTTAAAACAGATTGAGCAAAAGTCACGTTATTTACTCTTAGTGCTTGATGTTAAGGCTGATGAACTGCTTCCTTTCTCCTTTTTAGGTAGTTTAAGAAAGAGTCACATGCTTGAAGAAATGTTGTTAGAGCAGTAA", "aa": "MEKDKELVSSIVTAKSMIDRFFWSILSFLLTNLVFLFVAFLILIIYLISEITQQFAFAFIATIVFIIFYNILFLSYLLTMYIKGLKQIEQKSRYLLLVLDVKADELLPFSFLGSLRKSHMLEEMLLEQ", "aa_hexdigest": "a53a8e4bcd321239e32ee769b1a386f7", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD64DA", "ncbi_nrp_id": "WP_014574900.1", "uniref100_id": "UniRef100_A0A0H3DMB4", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD64DA", "RefSeq:WP_014574900.1", "UniRef:UniRef100_A0A0H3DMB4" ] }, "ips": { "uniref100_id": "UniRef100_A0A0H3DMB4", "uniref90_id": "UniRef90_P75582" }, "psc": { "uniref90_id": "UniRef90_P75582", "product": "MPN163 family protein", "uniref50_id": "UniRef50_Q9ZB80", "go_ids": [ "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_Q9ZB80", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9ZB80" ], "product": "Uncharacterized protein MG149.1" }, "genes": [], "id": "APKECPOBEN_416", "locus": "GCA002090215_00380" }, { "type": "cds", "sequence": "contig_1", "start": 217604, "stop": 217930, "strand": "+", "frame": 2, "gene": "rpsJ", "product": "30S ribosomal protein S10", "db_xrefs": [ "COG:COG0051", "COG:J", "GO:0000049", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0015935", "GO:0022627", "RefSeq:WP_010874521.1", "SO:0001217", "UniParc:UPI0000134BB6", "UniRef:UniRef100_P75581", "UniRef:UniRef50_O52331", "UniRef:UniRef90_P75581" ], "nt": "ATGAACGCCGCTAACGCAGTTAAGTACCCTGAGCTCAAGATTAAGCTCGAGTCCTATGACAGCACCCTTTTAGACCTCACTACGAAAAAGATAGTGGAGGTAGTAAAGGGTGTCGATGTCAAAATTAAAGGTCCATTGCCTCTACCTACCAAAAAAGAGGTAATTACGATCATCCGTTCACCCCATGTGGATAAAGCATCACGCGAACAGTTTGAAAAAAACCGCCACAAACGTTTAATGATCTTGGTGGATGTTAACCAAGGTGCGATTGACAGCTTGAAGCGGATCAAGATCCCCGTTGGAGTAACTTTAAGATTTTCAAAGTAG", "aa": "MNAANAVKYPELKIKLESYDSTLLDLTTKKIVEVVKGVDVKIKGPLPLPTKKEVITIIRSPHVDKASREQFEKNRHKRLMILVDVNQGAIDSLKRIKIPVGVTLRFSK", "aa_hexdigest": "24ae859f4f5db52be5dbf177e84f99ca", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000134BB6", "ncbi_nrp_id": "WP_010874521.1", "uniref100_id": "UniRef100_P75581", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000134BB6", "RefSeq:WP_010874521.1", "UniRef:UniRef100_P75581" ] }, "ips": { "uniref100_id": "UniRef100_P75581", "uniref90_id": "UniRef90_P75581" }, "psc": { "uniref90_id": "UniRef90_P75581", "gene": "rpsJ", "product": "30S ribosomal protein S10", "uniref50_id": "UniRef50_O52331", "cog_id": "COG0051", "cog_category": "J", "go_ids": [ "GO:0000049", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0015935", "GO:0022627" ] }, "pscc": { "uniref50_id": "UniRef50_O52331", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_O52331" ], "product": "Small ribosomal subunit protein uS10" }, "genes": [ "rpsJ" ], "id": "APKECPOBEN_417", "locus": "GCA002090215_00381" }, { "type": "cds", "sequence": "contig_1", "start": 217944, "stop": 218807, "strand": "+", "frame": 3, "gene": "rplC", "product": "50S ribosomal protein L3", "db_xrefs": [ "COG:COG0087", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022625", "KEGG:K02906", "RefSeq:WP_010874522.1", "SO:0001217", "UniParc:UPI000013409B", "UniRef:UniRef100_P75580", "UniRef:UniRef50_P75580", "UniRef:UniRef90_P75580" ], "nt": "ATGGAAATTCGTGGTATTTTTGGGGTTAAAGTAGGCATGAGCCAGGTGTTTACAACCAATAATGAGCGCCTACCTATTACCGTTATTTATTGTGAACCTAACCAGGTAGCAGGTGTTAAGACTGAGGCTAAGGACAAGTACAGTGCTACCTTGTTGAGTTTTGACACCGTTGAAAACAAAAAGCTCAACAAGCCTCAACAAGGCTTCTTTGAAAAAAACAACTTAAAGCCAACCAAGCACTTACAGGAAATCCGCAATATGACCGGGTTTGAAATGGGGCAACAAATCACACCGCAAAATCTCTTCCAAGTGGGTGAGTATGTTGATGTAAGTGCCATTAGTAAAGGTCGTGGGTTTACTGGTGCCATTAAGCGCTGGAACTTTAAGATCGGTCCGTTAGGTCATGGGGCTGGTTATCCCCACCGCTTCCAAGGTTCAGTCCAAGCTGGTCGTGGGGGTGCGAGTGCACAAAGAGTGTTTAAGGGCAAAAAGATGTCCGGACACTATGGTCATGAAAAAGTAACGGTACAAAACTTACGCATTGTTGGTTTTGATGAAGCTAACATGTTGGTATTAGTATCAGGGGCGATCGCAGGTCCTGAAGGTGGTGTGGTCTTAATCCGTACCGCTAAGAAAAAACCGGGGGTAGTGAAACCAATTGAGTTAGCTGTTCAAACCGAAAAGGCACCGGAAGCAAAACCGGCCAAGCTTTCCAAAAAGAAACAAGCCAAGGAGTTAGCGAAAGCACAAGCAGCTAACCAGCAAACTGTTGAAGCTAAGGTAGACACTCCAGTAGTAGAACCAAAACCAACTGAAGTTAAAAAAGCAGCACCAGTTGTAGAAAAGAAAGGGGAAGATAAATAA", "aa": "MEIRGIFGVKVGMSQVFTTNNERLPITVIYCEPNQVAGVKTEAKDKYSATLLSFDTVENKKLNKPQQGFFEKNNLKPTKHLQEIRNMTGFEMGQQITPQNLFQVGEYVDVSAISKGRGFTGAIKRWNFKIGPLGHGAGYPHRFQGSVQAGRGGASAQRVFKGKKMSGHYGHEKVTVQNLRIVGFDEANMLVLVSGAIAGPEGGVVLIRTAKKKPGVVKPIELAVQTEKAPEAKPAKLSKKKQAKELAKAQAANQQTVEAKVDTPVVEPKPTEVKKAAPVVEKKGEDK", "aa_hexdigest": "39a3d5e6da8eeebbd5991a719c1a0f3f", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013409B", "ncbi_nrp_id": "WP_010874522.1", "uniref100_id": "UniRef100_P75580", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013409B", "RefSeq:WP_010874522.1", "UniRef:UniRef100_P75580" ] }, "ips": { "uniref100_id": "UniRef100_P75580", "uniref90_id": "UniRef90_P75580" }, "psc": { "uniref90_id": "UniRef90_P75580", "gene": "rplC", "product": "50S ribosomal protein L3", "uniref50_id": "UniRef50_P75580", "cog_id": "COG0087", "cog_category": "J", "kegg_orthology_id": "K02906", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_P75580", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75580" ], "product": "Large ribosomal subunit protein uL3" }, "genes": [ "rplC" ], "id": "APKECPOBEN_418", "locus": "GCA002090215_00382" }, { "type": "cds", "sequence": "contig_1", "start": 218807, "stop": 218941, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTAAGCTTAAACTAATTAAAATCGACGGTTCCTTTGAAACTGAACCAGTGAAACTAAGCCCTGGTTTAATTGCTAAGGAACTCAAACAACAACCGGTGTTTGATGCCGTGTTAGTAGAACAAGCTTCCTGA", "aa": "MAKLKLIKIDGSFETEPVKLSPGLIAKELKQQPVFDAVLVEQAS", "aa_hexdigest": "606d57c287ba36a74d465242d00bebc1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4810.649100000002, "isoelectric_point": 6.090491294860839 }, "id": "APKECPOBEN_419", "locus": "GCA002090215_00383" }, { "type": "cds", "sequence": "contig_1", "start": 219074, "stop": 219445, "strand": "+", "frame": 2, "gene": "rplD", "product": "50S ribosomal protein L4", "db_xrefs": [ "COG:COG0088", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022625", "GO:0046677", "GO:1990904", "SO:0001217", "UniRef:UniRef50_P75579", "UniRef:UniRef90_P75579" ], "nt": "GTGTTTGGTCCCAAGCCCAATCGTAACTACAGCTTAAAACTCAACAAGAAAGCGCACACGGCAGCCTTGCACACTGTTTGGAGTGAAAAGTTAGCTTCAGACAACACCCACCTAGTGGACCAAAACCTCTTTAACAAAACTGAGGGTAAAACCAAGGTGATGATGCAATTTTTAAAGAGTGCCAAGCTCTTGGACAAAAACGTGTTGTTTGTAGTTAATACCCTTAACACCAACTTAGAACAAAGCACCAGCAACATTAAAAACGTGCAGGTCAAACACCTAGACAAGGTTTCTGTAAGGGATCTGATGTTAGCTAACGCTCTTTTAGTTGAAAAAGAAGTTTTAAAAGCTTTGGAGGGCAAATTTAAATAA", "aa": "MFGPKPNRNYSLKLNKKAHTAALHTVWSEKLASDNTHLVDQNLFNKTEGKTKVMMQFLKSAKLLDKNVLFVVNTLNTNLEQSTSNIKNVQVKHLDKVSVRDLMLANALLVEKEVLKALEGKFK", "aa_hexdigest": "f57f1e4b662ca76540be5a0e7bddb21c", "start_type": "GTG", "rbs_motif": null, "genes": [ "rplD" ], "truncated": "5-prime", "pseudogene": { "start": 218807, "stop": 219445, "observations": { "mutation": [ 218939 ], "stop": [ 218939 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75579", "query_cov": 1.0, "subject_cov": 0.5801886792452831, "identity": 0.992, "score": 235.0, "evalue": 5.38e-77, "gene_start": 1, "gene_end": 123, "reference_start": 90, "reference_end": 212, "reference_sequence": "MAKLKLIKIDGSFETEPVKLSPGLIAKELKQQPVFDAVLVEQASWRQGTHSILTKGEVRGGGKKPYKQKHTGKARQGSTRNPHFVGGGIVFGPKPNRNYSLKLNKKAHTAALHTVWSEKLASDNTHLVDQNLFNKTEGKTKVMMQFLKSAKLLDKNVLFVVNTLNTNLEQSTSNIKNVQVKHLDKVSVRDLMLANALLVEKEVLKALEGKFK" }, "paralog": false, "identity": 0.9952830188679245, "score": 414.0, "evalue": 5.88e-150, "description": "Internal stop codon at 218939. Nonsense mutation around 218939.", "psc": { "uniref90_id": "UniRef90_P75579", "gene": "rplD", "product": "50S ribosomal protein L4", "uniref50_id": "UniRef50_P75579", "cog_id": "COG0088", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022625", "GO:0046677", "GO:1990904" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75579", "UniRef:UniRef50_P75579", "COG:COG0088", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022625", "GO:0046677", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_P75579", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75579" ], "product": "Large ribosomal subunit protein uL4" } }, "id": "APKECPOBEN_420", "locus": "GCA002090215_00384" }, { "type": "cds", "sequence": "contig_1", "start": 219448, "stop": 220161, "strand": "+", "frame": 1, "gene": "rplW", "product": "Large ribosomal subunit protein uL23", "db_xrefs": [ "COG:COG0089", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904", "RefSeq:WP_010874524.1", "SO:0001217", "UniParc:UPI0000133DA4", "UniRef:UniRef100_P75578", "UniRef:UniRef50_P75578", "UniRef:UniRef90_P75578" ], "nt": "ATGGATGTAACGAACGTACTGCTAAAACCAGTTCTAACCGAAAAGGTGTACTTCAACCAAATGGGTGAAACCAAGAAGTACGTCTTTGTGGTGAACCCTAAAGCAAGTAAAACACGCGTTAAACTAGCTTTTGAATTAGTGTATGGCATTAAGCCACTGAAGGTTAACACCTTAATTAGAAAACCAACCACCATTCGCGGTGGGAGCCGTTTTCCTGGTTTGAGCAAACTAGAAAAGTTGGCTGTGATTACCTTACCAAAGGGTATTGCCATTTCAGTTACTGGTGAAGCGCCAGAAAAAACCGATAAGCCAGCGGACAAAACCACCCTTAAGGAAAGTACTGTTAAGGAGATTAAGGACACCAAAAACAGTCCAGAAGCTGTTGTGAAAACTGCTGTGGAAGCGTTGCAAATTAAACCAACTGCCGCTCCTGTAACCACCGCCCCTCTACAAACCGTGGCGGTGAAGGTAGCTAAAGAAGTTAAAGAAGTTAAGGTAGAAAAGCCGGTTAAAGTGGAAAAACCAACCAAACCGGCAAAGGTAGCAAAAGAAGCTAAAACGACCAAAGTAGCTAAGGAAACTAAAGCTGAAAAGTCAGTTCAAACAACCAAAGTAGCTAAGGAAACTAAAACTGAAAAGTCAGCTAAAACTACCAAAACTACAGCTACAAAAACTACTAAAACTAAAACCACCAAAAAGGAGGTTAAAAAGTAA", "aa": "MDVTNVLLKPVLTEKVYFNQMGETKKYVFVVNPKASKTRVKLAFELVYGIKPLKVNTLIRKPTTIRGGSRFPGLSKLEKLAVITLPKGIAISVTGEAPEKTDKPADKTTLKESTVKEIKDTKNSPEAVVKTAVEALQIKPTAAPVTTAPLQTVAVKVAKEVKEVKVEKPVKVEKPTKPAKVAKEAKTTKVAKETKAEKSVQTTKVAKETKTEKSAKTTKTTATKTTKTKTTKKEVKK", "aa_hexdigest": "d08555cd895ec3b00883a94da7e8e52e", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000133DA4", "ncbi_nrp_id": "WP_010874524.1", "uniref100_id": "UniRef100_P75578", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000133DA4", "RefSeq:WP_010874524.1", "UniRef:UniRef100_P75578" ] }, "ips": { "uniref100_id": "UniRef100_P75578", "uniref90_id": "UniRef90_P75578" }, "psc": { "uniref90_id": "UniRef90_P75578", "gene": "rplW", "product": "Large ribosomal subunit protein uL23", "uniref50_id": "UniRef50_P75578", "cog_id": "COG0089", "cog_category": "J", "go_ids": [ "GO:1990904", "GO:0005840", "GO:0019843", "GO:0003735", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_P75578", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75578" ], "product": "Large ribosomal subunit protein uL23" }, "genes": [ "rplW" ], "id": "APKECPOBEN_421", "locus": "GCA002090215_00385" }, { "type": "cds", "sequence": "contig_1", "start": 220161, "stop": 221024, "strand": "+", "frame": 3, "gene": "rplB", "product": "50S ribosomal protein L2", "db_xrefs": [ "COG:COG0090", "COG:J", "GO:0002181", "GO:0003723", "GO:0003735", "GO:0006412", "GO:0015934", "GO:0016740", "GO:0019843", "KEGG:K02886", "RefSeq:WP_010874525.1", "SO:0001217", "UniParc:UPI0000133ECF", "UniRef:UniRef100_P75577", "UniRef:UniRef50_Q9AMK8", "UniRef:UniRef90_P75577" ], "nt": "ATGCCTATTAAGAAAATTATTAGTCGTTCTAACAGTGGGATCCACCACTCCACGGTAATTGACTATAAGAAGTTATTAACCACCAATAAGAACAAACCAGAAAAGTCGTTGTTAGTTACCCTTAAAAAACACGGGGGTCGCAACAACCAAGGGAAAATTACGGTGCGCCACCAGGGTGGACGCAACAAGCGCAAGTACCGCATTATTGACTTTAAACGAACGCACTATGACAACATCGAAGCGACCGTTAAGTCGATCGAGTACGATCCGAACCGTTCTTGCTTTGTGTCGTTAATTACTTACGCTAACGGTGCCAAGAGTTACATCATTAGCCCTGATGGCATTAAGGTAGGTGATAAGATCTTGGCTTCCGAACACCCGATTGACATTAAACCGGGCTTTAGTATGCCCTTAGCCTTTATCCCTGAAGGTACCCAAGTGCATAACATTGAACTCCACCCTAAGGGAGGTGGTCAAATTGCCAGGAGTGCGGGGAGTTATGCCCGCATTTTGGGTCAAGACGAAACTGGTAAGTATGTCATTTTGCAACTTTTAAGTGGTGAAACACGCAAGTTCTTAAAGGAGTGCCGTGCTACTGTGGGAGTAGTTTCTAACTTAGACCACAACCTCGTAGTCATTGGTAAGGCTGGTCGCAACCGTCACCGCGGCATCCGTCCAACCGTTCGTGGTTCGGCCATGAACCCCAATGACCACCCCCACGGTGGTGGTGAAGGTCGTAGTCCCGTGGGTCGGGATGCACCCCGGACTCCATGGGGTAAGCGCCACATGGGTGTGAAGACCCGCAACATGAAGAAAGCATCCACTAACTTAATTATTCGTAACCGGAAAGGAGAACAATACTAA", "aa": "MPIKKIISRSNSGIHHSTVIDYKKLLTTNKNKPEKSLLVTLKKHGGRNNQGKITVRHQGGRNKRKYRIIDFKRTHYDNIEATVKSIEYDPNRSCFVSLITYANGAKSYIISPDGIKVGDKILASEHPIDIKPGFSMPLAFIPEGTQVHNIELHPKGGGQIARSAGSYARILGQDETGKYVILQLLSGETRKFLKECRATVGVVSNLDHNLVVIGKAGRNRHRGIRPTVRGSAMNPNDHPHGGGEGRSPVGRDAPRTPWGKRHMGVKTRNMKKASTNLIIRNRKGEQY", "aa_hexdigest": "84ec1e77c99929a1da5f4d6fe0e5aad7", "start_type": "ATG", "rbs_motif": "AAAA", "ups": { "uniparc_id": "UPI0000133ECF", "ncbi_nrp_id": "WP_010874525.1", "uniref100_id": "UniRef100_P75577", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000133ECF", "RefSeq:WP_010874525.1", "UniRef:UniRef100_P75577" ] }, "ips": { "uniref100_id": "UniRef100_P75577", "uniref90_id": "UniRef90_P75577" }, "psc": { "uniref90_id": "UniRef90_P75577", "gene": "rplB", "product": "50S ribosomal protein L2", "uniref50_id": "UniRef50_Q9AMK8", "cog_id": "COG0090", "cog_category": "J", "kegg_orthology_id": "K02886", "go_ids": [ "GO:0002181", "GO:0003723", "GO:0003735", "GO:0006412", "GO:0015934", "GO:0016740", "GO:0019843" ] }, "pscc": { "uniref50_id": "UniRef50_Q9AMK8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9AMK8" ], "product": "Large ribosomal subunit protein uL2" }, "genes": [ "rplB" ], "id": "APKECPOBEN_422", "locus": "GCA002090215_00386" }, { "type": "cds", "sequence": "contig_1", "start": 221024, "stop": 221287, "strand": "+", "frame": 2, "gene": "rpsS", "product": "30S ribosomal protein S19", "db_xrefs": [ "COG:COG0185", "COG:J", "GO:0000028", "GO:0003735", "GO:0006412", "GO:0015935", "GO:0019843", "GO:0022627", "RefSeq:WP_010874526.1", "SO:0001217", "UniParc:UPI0000134DEC", "UniRef:UniRef100_P75576", "UniRef:UniRef50_Q6ME59", "UniRef:UniRef90_P75576" ], "nt": "ATGTCACGCAGTGCTAAAAAAGGAGCCTTTGTTGATGCTCACCTCTTAAAGAAAGTGATCGATATGAACAAACAAGAGAAAAAACGTCCGATCAAAACCTGGTCCCGTCGTAGTACCATCTTCCCCGAATTTGTCGGTAATACCTTTGCTGTACATAACGGCAAGACCTTCATTAATGTTTATGTAACTGATGACATGGTGGGGCACAAACTCGGTGAGTTCTCCCCGACCCGTAACTTCAAACAACACACCGCTAACCGTTAG", "aa": "MSRSAKKGAFVDAHLLKKVIDMNKQEKKRPIKTWSRRSTIFPEFVGNTFAVHNGKTFINVYVTDDMVGHKLGEFSPTRNFKQHTANR", "aa_hexdigest": "9fa790bb912197d37c1ffb6630f98a3c", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000134DEC", "ncbi_nrp_id": "WP_010874526.1", "uniref100_id": "UniRef100_P75576", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000134DEC", "RefSeq:WP_010874526.1", "UniRef:UniRef100_P75576" ] }, "ips": { "uniref100_id": "UniRef100_P75576", "uniref90_id": "UniRef90_P75576" }, "psc": { "uniref90_id": "UniRef90_P75576", "gene": "rpsS", "product": "30S ribosomal protein S19", "uniref50_id": "UniRef50_Q6ME59", "cog_id": "COG0185", "cog_category": "J", "go_ids": [ "GO:0000028", "GO:0003735", "GO:0006412", "GO:0015935", "GO:0019843", "GO:0022627" ] }, "pscc": { "uniref50_id": "UniRef50_Q6ME59", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q6ME59" ], "product": "Small ribosomal subunit protein uS19" }, "genes": [ "rpsS" ], "id": "APKECPOBEN_423", "locus": "GCA002090215_00387" }, { "type": "cds", "sequence": "contig_1", "start": 221289, "stop": 221768, "strand": "+", "frame": 3, "gene": "rplV", "product": "50S ribosomal protein L22", "db_xrefs": [ "GO:0003735", "GO:0006412", "GO:0015934", "GO:0019843", "GO:0022625", "GO:0046677", "RefSeq:WP_015344888.1", "SO:0001217", "UniParc:UPI0000133D7A", "UniRef:UniRef100_P75575", "UniRef:UniRef50_P75575", "UniRef:UniRef90_P75575" ], "nt": "ATGATCGCCTTTGCAAAACAATTCCGTGTGAGAATCTCACCCCAAAAAGCCCGCTTAGTGTGTCAACTCATTGTGGGTAAGAAAACCGCTGATGCCCAAAACATCCTTTCCAATACACCCAAAAAAGCGGCTACCTTAATCGCTAAACTACTCAACAGTGCGATTGCCAACGCTACCAATAACCACGGCATGAATGGGGATGCTCTTTATGTCTTTGAGTGTGTGGCTAACCAAGGTCCGAGTATGAAGCGCACTATTCCCCGCGCTAAGGGCTCGAGTAACATGATTACCAAGCGCTCTTCTAATTTAGTGGTGAAACTATCGGATAACCCGAATGAACGTCAAGAATTAATTAAACAACAAAAGGCCCTAGTTAAAAAACGGGTCGAAGGTCAACAAAAAGCAAAGATGGCACGTCAAAAAGCAGTAACATCAGTGGTAAAAGCGCCAAGTAAAACTCAAGGAGGTGTACAAAAGTAA", "aa": "MIAFAKQFRVRISPQKARLVCQLIVGKKTADAQNILSNTPKKAATLIAKLLNSAIANATNNHGMNGDALYVFECVANQGPSMKRTIPRAKGSSNMITKRSSNLVVKLSDNPNERQELIKQQKALVKKRVEGQQKAKMARQKAVTSVVKAPSKTQGGVQK", "aa_hexdigest": "6c46131b06b5002f01fe4f2a9194dbb3", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000133D7A", "ncbi_nrp_id": "WP_015344888.1", "uniref100_id": "UniRef100_P75575", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000133D7A", "RefSeq:WP_015344888.1", "UniRef:UniRef100_P75575" ] }, "ips": { "uniref100_id": "UniRef100_P75575", "uniref90_id": "UniRef90_P75575" }, "psc": { "uniref90_id": "UniRef90_P75575", "gene": "rplV", "product": "50S ribosomal protein L22", "uniref50_id": "UniRef50_P75575", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015934", "GO:0019843", "GO:0022625", "GO:0046677" ] }, "pscc": { "uniref50_id": "UniRef50_P75575", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75575" ], "product": "Large ribosomal subunit protein uL22" }, "genes": [ "rplV" ], "id": "APKECPOBEN_424", "locus": "GCA002090215_00388" }, { "type": "cds", "sequence": "contig_1", "start": 221935, "stop": 222591, "strand": "+", "frame": 1, "gene": "rpsC", "product": "30S ribosomal protein S3", "db_xrefs": [ "COG:COG0092", "COG:J", "GO:0003729", "GO:0003735", "GO:0006412", "GO:0019843", "GO:0022627", "KEGG:K02982", "SO:0001217", "UniRef:UniRef50_O52338", "UniRef:UniRef90_P41205" ], "nt": "GTGGAGATTGAACGTACTCAAGCAACCGTGGACGTATTTGTGTACGCCGCCCAACCCGCTTTTCTCATTGGTAGTGAAAACAAGAACATCCAAAAGATCACCAAGCAAATTAAGCAAATTATTGGTCGTACCACCAATTTAGACCTCACCATTAACGAAATTGGCTCCCCTATGTTATCAGCGCGCATTATTGCGCGGGATCTAGCCAACGCGATTGAAGCACGGGTACCGTTACGAACGGCAATGCGCCAATCCTTAATTAAGGTGTTGAAGGCTGGCGCTAACGGGATTAAGGTGTTGGTATCGGGTCGGTTAAACGGTGCCGAAATTGCGCGCGACAAGATGTACATCGAGGGCAATATGCCACTGTCAACCCTGCGTGCTGACATTGACTATGCCTTGGAAAAAGCGCAAACTACGTACGGTGTCATTGGTGTTAAGGTATGGATTAATCGCGGCATGATTTACACCAAGGGCTTAAACCGCACCCCGGCCCACATTCTCCACCCACAAAAGAAACAACCAAACCGCCAAAACCAACAACCACGCCACTTTAACCAGGGCCAGGTGTTGAGTGCTAATAAGTTAACTGGTAGTGATGTGGAGACCAGCAGTATCCAAGCACTTACTAAGCCTAACAAGGAGGACAAACAATAA", "aa": "MEIERTQATVDVFVYAAQPAFLIGSENKNIQKITKQIKQIIGRTTNLDLTINEIGSPMLSARIIARDLANAIEARVPLRTAMRQSLIKVLKAGANGIKVLVSGRLNGAEIARDKMYIEGNMPLSTLRADIDYALEKAQTTYGVIGVKVWINRGMIYTKGLNRTPAHILHPQKKQPNRQNQQPRHFNQGQVLSANKLTGSDVETSSIQALTKPNKEDKQ", "aa_hexdigest": "93133787fde845f73905639b469b8ff7", "start_type": "GTG", "rbs_motif": null, "genes": [ "rpsC" ], "truncated": "5-prime", "pseudogene": { "start": 221770, "stop": 222591, "observations": { "mutation": [ 221833 ], "stop": [ 221833 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P41205", "query_cov": 1.0, "subject_cov": 0.7985347985347986, "identity": 0.995, "score": 413.0, "evalue": 6.56e-145, "gene_start": 1, "gene_end": 218, "reference_start": 56, "reference_end": 273, "reference_sequence": "MGQKVNSNGLRFGINKNWISRWTANSHAQTAKWLIEDEKIRNLFFVNYRNAQVSNVEIERTQATVDVFVYAAQPAFLIGSENKNIQKITKQIKQIIGRTTNLDLTINEIGSPMLSARIIARDLANAIEARVPLRTAMRQSLIKVLKAGANGIKVLVSGRLNGAEIARDKMYIEGNMPLSTLRADIDYALEKAQTTYGVIGVKVWINRGMIYTKGLNRTPAHILHPQKKQPNRQNQQPRHFNQGQVLSANKLTGSDVETSSIQALTKPNKEDKQ" }, "paralog": false, "identity": 0.9963369963369964, "score": 532.0, "evalue": 3.77e-194, "description": "Internal stop codon at 221833. Nonsense mutation around 221833.", "psc": { "uniref90_id": "UniRef90_P41205", "gene": "rpsC", "product": "30S ribosomal protein S3", "uniref50_id": "UniRef50_O52338", "cog_id": "COG0092", "cog_category": "J", "kegg_orthology_id": "K02982", "go_ids": [ "GO:0003729", "GO:0003735", "GO:0006412", "GO:0019843", "GO:0022627" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P41205", "UniRef:UniRef50_O52338", "COG:COG0092", "COG:J", "KEGG:K02982", "GO:0003729", "GO:0003735", "GO:0006412", "GO:0019843", "GO:0022627" ] }, "pscc": { "uniref50_id": "UniRef50_O52338", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_O52338" ], "product": "Small ribosomal subunit protein uS3" } }, "id": "APKECPOBEN_425", "locus": "GCA002090215_00389" }, { "type": "cds", "sequence": "contig_1", "start": 222591, "stop": 222977, "strand": "+", "frame": 3, "gene": "rplP", "product": "50S ribosomal protein L16", "db_xrefs": [ "COG:COG0197", "COG:J", "GO:0000049", "GO:0003735", "GO:0006412", "GO:0019843", "GO:0022625", "KEGG:K02878", "SO:0001217", "UniRef:UniRef50_P02415", "UniRef:UniRef90_P47404" ], "nt": "ATGTTACAACCAAAAAGAACAAAGTACCGCAAACCTCACAACGTGAGTTACGAGGGCAAGGCTAAGGGCAACAGCTATGTTGCTTTTGGTGAATATGGTCTAGTAGCTACTAAGGGTAACTGGATCGATGCCCGTGCCATAGAATCAGCCCGGATTGCCATTAGTAAGTGTTTGGGTAAAACCGGTAAGATGTGGATCCGCATCTTCCCCCACATGTCCAAGACTAAAAAACCGTTAGAAGTACGGATGGGTTCTGGTAAGGGTAACCCGGAATTCTGGGTAGCAGTAGTAAAACAGGGCACGGTGATGTTTGAGGTAGCTAACATCCCTGAAAGCCAAATGATTAAGGCTCTAACCCGTGCTGGTCACAAGTTACCCGTGACCTGA", "aa": "MLQPKRTKYRKPHNVSYEGKAKGNSYVAFGEYGLVATKGNWIDARAIESARIAISKCLGKTGKMWIRIFPHMSKTKKPLEVRMGSGKGNPEFWVAVVKQGTVMFEVANIPESQMIKALTRAGHKLPVT", "aa_hexdigest": "a065ea1cc9e1d1d733ac3d40561c3b20", "start_type": "ATG", "rbs_motif": "AACAA", "psc": { "uniref90_id": "UniRef90_P47404", "query_cov": 1.0, "subject_cov": 0.920863309352518, "identity": 1.0, "score": 262.0, "evalue": 1.23e-88, "valid": true, "gene": "rplP", "product": "50S ribosomal protein L16", "uniref50_id": "UniRef50_P02415", "cog_id": "COG0197", "cog_category": "J", "kegg_orthology_id": "K02878", "go_ids": [ "GO:0000049", "GO:0003735", "GO:0006412", "GO:0019843", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_P02415", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P02415" ], "product": "Large ribosomal subunit protein uL16" }, "genes": [ "rplP" ], "id": "APKECPOBEN_426", "locus": "GCA002090215_00390" }, { "type": "cds", "sequence": "contig_1", "start": 223010, "stop": 223204, "strand": "+", "frame": 2, "gene": "rpmC", "product": "50S ribosomal protein L29", "db_xrefs": [ "COG:COG0255", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625", "SO:0001217", "UniRef:UniRef50_Q50310", "UniRef:UniRef90_Q50310" ], "nt": "ATGACCGTTGCAAAAGAATTACGCCAAAAGAGTTCCGAAGAGTTGGTAAAACTCGTGATTAAGCTCAAAGGGGAACTGTTAGAGTACCGCTTTAAGTTAGCACACGGGGAGCTAGACAAACCGCACTTAATTAACCAAACCAGGCGGTTATTGGCTACCATTTTAACGATTCTTACCGAGCGTAAGTTGAACTGA", "aa": "MTVAKELRQKSSEELVKLVIKLKGELLEYRFKLAHGELDKPHLINQTRRLLATILTILTERKLN", "aa_hexdigest": "3c1ca8354d0101d23fdc06853121825e", "start_type": "ATG", "rbs_motif": null, "genes": [ "rpmC" ], "truncated": "3-prime", "pseudogene": { "start": 223010, "stop": 223345, "observations": { "mutation": [ 223202 ], "stop": [ 223202 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q50310", "query_cov": 1.0, "subject_cov": 0.5765765765765766, "identity": 1.0, "score": 119.0, "evalue": 9.11e-34, "gene_start": 1, "gene_end": 64, "reference_start": 1, "reference_end": 64, "reference_sequence": "MTVAKELRQKSSEELVKLVIKLKGELLEYRFKLAHGELDKPHLINQTRRLLATILTILTERKLNWQEEQAKYKLLTKKTNEAAVNAWKQHLEANKAKLLKSRAKREDASKK" }, "paralog": false, "identity": 0.990990990990991, "score": 210.0, "evalue": 5.6e-72, "description": "Internal stop codon at 223202. Nonsense mutation around 223202.", "psc": { "uniref90_id": "UniRef90_Q50310", "gene": "rpmC", "product": "50S ribosomal protein L29", "uniref50_id": "UniRef50_Q50310", "cog_id": "COG0255", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q50310", "UniRef:UniRef50_Q50310", "COG:COG0255", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_Q50310", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50310" ], "product": "Large ribosomal subunit protein uL29" } }, "id": "APKECPOBEN_427", "locus": "GCA002090215_00391" }, { "type": "cds", "sequence": "contig_1", "start": 223345, "stop": 223602, "strand": "+", "frame": 1, "gene": "rpsQ", "product": "30S ribosomal protein S17", "db_xrefs": [ "COG:COG0186", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022627", "RefSeq:WP_010874531.1", "SO:0001217", "UniParc:UPI0000134D81", "UniRef:UniRef100_Q50309", "UniRef:UniRef50_P47406", "UniRef:UniRef90_Q50309" ], "nt": "ATGAAACGTAATCAAAGAAAGGTCCTAATTGGGATTGTTAAAAGTACTAAAAACGCCAAGACCGCTACGGTTCAAGTAGAATCACGGTTTAAGCACCCTTTGTACCATAAGTCCGTAGTGCGACACAAGAAGTACCAAGCCCACAATGAGGGTGAAGTACTCGCTAAGGACGGGGACAAGGTACAAATTGTGGAAACGCGACCGTTGTCTGCAACCAAGCGCTTCCGCATTGCCAAAATTATTGAGAGGGCTAAATAG", "aa": "MKRNQRKVLIGIVKSTKNAKTATVQVESRFKHPLYHKSVVRHKKYQAHNEGEVLAKDGDKVQIVETRPLSATKRFRIAKIIERAK", "aa_hexdigest": "e161642a0296efaf2e1a4c9286c37bc5", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000134D81", "ncbi_nrp_id": "WP_010874531.1", "uniref100_id": "UniRef100_Q50309", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000134D81", "RefSeq:WP_010874531.1", "UniRef:UniRef100_Q50309" ] }, "ips": { "uniref100_id": "UniRef100_Q50309", "uniref90_id": "UniRef90_Q50309" }, "psc": { "uniref90_id": "UniRef90_Q50309", "gene": "rpsQ", "product": "30S ribosomal protein S17", "uniref50_id": "UniRef50_P47406", "cog_id": "COG0186", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022627" ] }, "pscc": { "uniref50_id": "UniRef50_P47406", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47406" ], "product": "Small ribosomal subunit protein uS17" }, "genes": [ "rpsQ" ], "id": "APKECPOBEN_428", "locus": "GCA002090215_00392" }, { "type": "cds", "sequence": "contig_1", "start": 223618, "stop": 223974, "strand": "+", "frame": 1, "gene": "rplN", "product": "50S ribosomal protein L14", "db_xrefs": [ "COG:COG0093", "COG:J", "GO:0003735", "GO:0006412", "GO:0015934", "GO:0022625", "GO:0070180", "KEGG:K02874", "SO:0001217", "UniRef:UniRef50_Q5SHP8", "UniRef:UniRef90_P47407" ], "nt": "ATGACAAGACTAAATGTTGCCGATAACACGGGCGCCAAGCAAGTGGGCATCATTAAGGTATTAGGTTCCACCCGGAAACGTTATGCCTTTTTGGGTGATGTGGTAGTGGTATCAGTTAAGGATGCTATCCCTAGCGGTATGGTCAAAAAGGGTCAAGTGCTACGGGCAGTAATTGTCCGCACTAAAAAGGGCCAACAACGTAAGGATGGTACCCACCTTAAGTTTGATGACAACGCTTGTGTGTTAATTAAGGAAGACAAGTCACCAAGAGGCACACGGATCTTTGGTCCGGTAGCGCGTGAACTACGGGAACGTGGTTACAACAAAATTTTGAGTTTAGCAGTGGAGGTAGTGTAA", "aa": "MTRLNVADNTGAKQVGIIKVLGSTRKRYAFLGDVVVVSVKDAIPSGMVKKGQVLRAVIVRTKKGQQRKDGTHLKFDDNACVLIKEDKSPRGTRIFGPVARELRERGYNKILSLAVEVV", "aa_hexdigest": "1d4d34751b9f8d42d5606a6c0bb09451", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47407", "query_cov": 1.0, "subject_cov": 0.9672131147540983, "identity": 0.9490000000000001, "score": 220.0, "evalue": 1.37e-72, "valid": true, "gene": "rplN", "product": "50S ribosomal protein L14", "uniref50_id": "UniRef50_Q5SHP8", "cog_id": "COG0093", "cog_category": "J", "kegg_orthology_id": "K02874", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015934", "GO:0022625", "GO:0070180" ] }, "pscc": { "uniref50_id": "UniRef50_Q5SHP8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q5SHP8" ], "product": "Large ribosomal subunit protein uL14" }, "genes": [ "rplN" ], "id": "APKECPOBEN_429", "locus": "GCA002090215_00393" }, { "type": "cds", "sequence": "contig_1", "start": 223974, "stop": 224309, "strand": "+", "frame": 3, "gene": "rplX", "product": "50S ribosomal protein L24", "db_xrefs": [ "COG:COG0198", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904", "RefSeq:WP_010874533.1", "SO:0001217", "UniParc:UPI0000133DCD", "UniRef:UniRef100_Q50307", "UniRef:UniRef50_O52343", "UniRef:UniRef90_Q50307" ], "nt": "ATGCAACGAATTAAAAAAGGTGATAAAGTAGTCGTAATTACCGGTAAGAATAAGGGTGGTAGTGGCATTGTTTTGAAAATTATGCCCGCGCGCCAACAAGCAATTGTAGAGGGACTCAACAAAGTAACACGCCACAAAAAGAAGGACCAAACCACAAAGCGCGCTGCTAAACAATCGACCGGTAAAGTGCAACAAGAAGCGCCAATCTTCTTGAGCAAGTTAGCTTTATTTGACCAAAAGGCAAAACAACAAACAATTGGCAAAATTAAGTACGTTATGGACCCTAAGACTAATAAGAAGACCCGGGTCTTTAAGAAATCCAACAACACGCTCTAG", "aa": "MQRIKKGDKVVVITGKNKGGSGIVLKIMPARQQAIVEGLNKVTRHKKKDQTTKRAAKQSTGKVQQEAPIFLSKLALFDQKAKQQTIGKIKYVMDPKTNKKTRVFKKSNNTL", "aa_hexdigest": "b672114d9ef8bdd0628ab567967334f1", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000133DCD", "ncbi_nrp_id": "WP_010874533.1", "uniref100_id": "UniRef100_Q50307", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000133DCD", "RefSeq:WP_010874533.1", "UniRef:UniRef100_Q50307" ] }, "ips": { "uniref100_id": "UniRef100_Q50307", "uniref90_id": "UniRef90_Q50307" }, "psc": { "uniref90_id": "UniRef90_Q50307", "gene": "rplX", "product": "50S ribosomal protein L24", "uniref50_id": "UniRef50_O52343", "cog_id": "COG0198", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_O52343", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_O52343" ], "product": "Large ribosomal subunit protein uL24" }, "genes": [ "rplX" ], "id": "APKECPOBEN_430", "locus": "GCA002090215_00394" }, { "type": "cds", "sequence": "contig_1", "start": 224312, "stop": 224854, "strand": "+", "frame": 2, "gene": "rplE", "product": "50S ribosomal protein L5", "db_xrefs": [ "COG:COG0094", "COG:J", "GO:0000049", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904", "KEGG:K02931", "RefSeq:WP_010874534.1", "SO:0001217", "UniParc:UPI0000134132", "UniRef:UniRef100_Q50306", "UniRef:UniRef50_Q50306", "UniRef:UniRef90_Q50306" ], "nt": "ATGAATAATCTCAAAGCACATTACCAAAAAACAATTGCCAAAGAGCTGCAAAAGAGCTTTGCGTTTTCCTCGATTATGCAAGTACCGCGCTTGGAAAAAATTGTGATTAACATGGGGGTGGGTGACGCCATTCGCGACAGTAAGTTCTTGGAATCAGCCTTAAACGAACTCCACCTCATTAGCGGTCAAAAACCAGTTGCTACTAAGGCTAAAAACGCCATTTCCACCTATAAGCTGCGCGCTGGTCAACTAATCGGTTGTAAGGTTACCTTGCGCGGTGAGCGGATGTGGGCCTTTTTGGAAAAACTGATTTATGTTGCCCTACCTCGGGTGCGGGATTTCCGCGGCCTTTCTTTAAAGTCTTTTGATGGTCGGGGTAACTATACCATTGGAATTAAAGAACAAATTATTTTCCCGGAAATTGTCTATGACGACATTAAACGGATCCGCGGGTTTGACGTTACTCTGGTAACTTCAACCAATAAGGACAGCGAAGCATTAGCTTTACTTAGAGCGTTAAACCTCCCACTTGTAAAAGGATAA", "aa": "MNNLKAHYQKTIAKELQKSFAFSSIMQVPRLEKIVINMGVGDAIRDSKFLESALNELHLISGQKPVATKAKNAISTYKLRAGQLIGCKVTLRGERMWAFLEKLIYVALPRVRDFRGLSLKSFDGRGNYTIGIKEQIIFPEIVYDDIKRIRGFDVTLVTSTNKDSEALALLRALNLPLVKG", "aa_hexdigest": "edbdda012ca37706b0304b21ef094906", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000134132", "ncbi_nrp_id": "WP_010874534.1", "uniref100_id": "UniRef100_Q50306", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000134132", "RefSeq:WP_010874534.1", "UniRef:UniRef100_Q50306" ] }, "ips": { "uniref100_id": "UniRef100_Q50306", "uniref90_id": "UniRef90_Q50306" }, "psc": { "uniref90_id": "UniRef90_Q50306", "gene": "rplE", "product": "50S ribosomal protein L5", "uniref50_id": "UniRef50_Q50306", "cog_id": "COG0094", "cog_category": "J", "kegg_orthology_id": "K02931", "go_ids": [ "GO:0000049", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_Q50306", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50306" ], "product": "Large ribosomal subunit protein uL5" }, "genes": [ "rplE" ], "id": "APKECPOBEN_431", "locus": "GCA002090215_00395" }, { "type": "cds", "sequence": "contig_1", "start": 224856, "stop": 225038, "strand": "+", "frame": 3, "gene": "rpsZ", "product": "type Z 30S ribosomal protein S14", "db_xrefs": [ "COG:COG0199", "COG:J", "GO:0003735", "GO:0005737", "GO:0005840", "GO:0006412", "GO:0008270", "GO:0015935", "GO:0019843", "KEGG:K02954", "SO:0001217", "UniRef:UniRef50_P12878", "UniRef:UniRef90_P47410" ], "nt": "ATGGCCAAAAAATCATTAAAAGTAAAACAAACTAGAATCCCCAAATTTGCCGTACGGGCTTACACCCGCTGTCAGCGTTGTGGTCGAGCCCGGGCAGTTTTAAGCCACTTTGGTGTGTGCCGTTTGTGCTTCCGCGAACTAGCATACGCTGGCGCAATTCCAGGAGTTAAAAAAGCATCATGA", "aa": "MAKKSLKVKQTRIPKFAVRAYTRCQRCGRARAVLSHFGVCRLCFRELAYAGAIPGVKKAS", "aa_hexdigest": "e4be5aeb126691b9d760f7afa251758a", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47410", "query_cov": 1.0, "subject_cov": 0.9836065573770492, "identity": 0.9, "score": 103.0, "evalue": 3.34e-28, "valid": true, "gene": "rpsZ", "product": "type Z 30S ribosomal protein S14", "uniref50_id": "UniRef50_P12878", "cog_id": "COG0199", "cog_category": "J", "kegg_orthology_id": "K02954", "go_ids": [ "GO:0003735", "GO:0005737", "GO:0005840", "GO:0006412", "GO:0008270", "GO:0015935", "GO:0019843" ] }, "pscc": { "uniref50_id": "UniRef50_P12878", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P12878" ], "product": "Small ribosomal subunit protein uS14B" }, "genes": [ "rpsZ" ], "id": "APKECPOBEN_432", "locus": "GCA002090215_00396" }, { "type": "cds", "sequence": "contig_1", "start": 225035, "stop": 225463, "strand": "+", "frame": 2, "gene": "rpsH", "product": "30S ribosomal protein S8", "db_xrefs": [ "COG:COG0096", "COG:J", "GO:0003735", "GO:0005737", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904", "RefSeq:WP_010874536.1", "SO:0001217", "UniParc:UPI000013516F", "UniRef:UniRef100_Q50304", "UniRef:UniRef50_Q9PQP6", "UniRef:UniRef90_Q50304" ], "nt": "ATGATAACAACTACAAAACCAATTAAAGCCCACTTTGATCCAGTGGCTGACTTACTAACGAAGATTAACAATGCGCGCAAAGCGAAGTTAATGACGGTAACCACAATTGCTTCCAAGCTCAAGATTGCCATCTTGGAAATTTTGGTCAAGGAAGGTTATTTAGCTAACTTCCAGGTCTTGGAAAACAAGAGCAAGACCAAGCGGATTGTCACCTTTAACTTAAAATACACGCAGCGCCGGATTCCCTCCATTAACGGGGTGAAACAAATTTCCAAGCCGGGTCTGCGGATCTACCGGCCCTTTGAAAAGCTCCCGTTAGTTTTAAACGGTCTTGGGATTGCCATTATTTCCACTAGTGATGGCGTGATGACTGATAAGGTAGCGCGCTTAAAGAAAATTGGAGGGGAGATTCTCGCTTACGTTTGGTAA", "aa": "MITTTKPIKAHFDPVADLLTKINNARKAKLMTVTTIASKLKIAILEILVKEGYLANFQVLENKSKTKRIVTFNLKYTQRRIPSINGVKQISKPGLRIYRPFEKLPLVLNGLGIAIISTSDGVMTDKVARLKKIGGEILAYVW", "aa_hexdigest": "7d47000330783529090b357afb242771", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013516F", "ncbi_nrp_id": "WP_010874536.1", "uniref100_id": "UniRef100_Q50304", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013516F", "RefSeq:WP_010874536.1", "UniRef:UniRef100_Q50304" ] }, "ips": { "uniref100_id": "UniRef100_Q50304", "uniref90_id": "UniRef90_Q50304" }, "psc": { "uniref90_id": "UniRef90_Q50304", "gene": "rpsH", "product": "30S ribosomal protein S8", "uniref50_id": "UniRef50_Q9PQP6", "cog_id": "COG0096", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005737", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQP6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQP6" ], "product": "Small ribosomal subunit protein uS8" }, "genes": [ "rpsH" ], "id": "APKECPOBEN_433", "locus": "GCA002090215_00397" }, { "type": "cds", "sequence": "contig_1", "start": 225470, "stop": 225931, "strand": "+", "frame": 2, "gene": "rplF", "product": "50S ribosomal protein L6", "db_xrefs": [ "COG:COG0097", "COG:J", "GO:0002181", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022625", "SO:0001217", "UniRef:UniRef50_P47412", "UniRef:UniRef90_Q50303" ], "nt": "ATGTCAAAAATAGGAAACAGAACGATTACACTAGACCCCGCTAAGGTCAACCTTAACTTCCAGAAGGACCACATTGCCGTTAAGGGTCCTTTGGGTCAAATTGAGCTCAAACTACCCCCTAACTTACCGCTCAAGTTTGAACTGAAGGATAATAACCTCCAAATTACGCGGAACAACGAGTTAAAGCAATCCAAGATCTTCCATGGTACTTACAACGCGTTAATTACCAACGCCATTATTGGGGTTACTCAAGGCTTTGAAAAGAAGCTGCGCTTAGTCGGTGTGGGTTACCGTGCTAACGTGGAAGGGGAAACGCTCAACCTCCAGTTGGGTTACTCCCATCCCATTAAGGAAAAGATTCCCAAAGGCTTAACAGTTAAAGTAGAAAAGAACACCGAGATTACCATTTCGGGAATTAGTAAGGAGTTAGTGGGTCAGTTTGCCACTGAGGTGCGCAAGTGA", "aa": "MSKIGNRTITLDPAKVNLNFQKDHIAVKGPLGQIELKLPPNLPLKFELKDNNLQITRNNELKQSKIFHGTYNALITNAIIGVTQGFEKKLRLVGVGYRANVEGETLNLQLGYSHPIKEKIPKGLTVKVEKNTEITISGISKELVGQFATEVRK", "aa_hexdigest": "148b1fbb56239c07daf79d0ce4fc7212", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_Q50303", "query_cov": 1.0, "subject_cov": 0.8315217391304348, "identity": 1.0, "score": 302.0, "evalue": 2.22e-103, "valid": true, "gene": "rplF", "product": "50S ribosomal protein L6", "uniref50_id": "UniRef50_P47412", "cog_id": "COG0097", "cog_category": "J", "go_ids": [ "GO:0002181", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_P47412", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47412" ], "product": "Large ribosomal subunit protein uL6" }, "genes": [ "rplF" ], "id": "APKECPOBEN_434", "locus": "GCA002090215_00398" }, { "type": "cds", "sequence": "contig_1", "start": 226025, "stop": 226375, "strand": "+", "frame": 2, "gene": "rplR", "product": "50S ribosomal protein L18", "db_xrefs": [ "COG:COG0256", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0008097", "GO:0022625", "RefSeq:WP_010874538.1", "SO:0001217", "UniParc:UPI0000133C7F", "UniRef:UniRef100_Q50302", "UniRef:UniRef50_Q9PQP4", "UniRef:UniRef90_Q50302" ], "nt": "ATGAAAACACGAACAGAACAACGGCGCTTACGCCATAAAAGGATTGTGAAGAAGATCCGCGCCACTAACCACGACAACCGCGTTGTGTTAATGGTGATTAAGTCCTTAAACCATATTAGTGTGCAAGCGTGGGACTTTTCCCAGAACATTGTTTTAGCGTCCTCTTCCTCACTTGCTTTAAAATTAAAGAATGGTAACAAGGACAATGCCAAGTTAGTGGGGCAAGACATTGCTGATAAACTAGTGAAGCTCAAACTAACCAACGTTGTCTTTGACACTGGTGGTAGTAAGTACCACGGCCGGATTGCGGCATTGGCCGAAGCAGCCCGCGAAAGGGGGTTAAACTTCTAA", "aa": "MKTRTEQRRLRHKRIVKKIRATNHDNRVVLMVIKSLNHISVQAWDFSQNIVLASSSSLALKLKNGNKDNAKLVGQDIADKLVKLKLTNVVFDTGGSKYHGRIAALAEAARERGLNF", "aa_hexdigest": "32ec61768f43ea6307058b5153b62ce2", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000133C7F", "ncbi_nrp_id": "WP_010874538.1", "uniref100_id": "UniRef100_Q50302", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000133C7F", "RefSeq:WP_010874538.1", "UniRef:UniRef100_Q50302" ] }, "ips": { "uniref100_id": "UniRef100_Q50302", "uniref90_id": "UniRef90_Q50302" }, "psc": { "uniref90_id": "UniRef90_Q50302", "gene": "rplR", "product": "50S ribosomal protein L18", "uniref50_id": "UniRef50_Q9PQP4", "cog_id": "COG0256", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0008097", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQP4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQP4" ], "product": "Large ribosomal subunit protein uL18" }, "genes": [ "rplR" ], "id": "APKECPOBEN_435", "locus": "GCA002090215_00399" }, { "type": "cds", "sequence": "contig_1", "start": 226379, "stop": 227038, "strand": "+", "frame": 2, "gene": "rpsE", "product": "30S ribosomal protein S5", "db_xrefs": [ "COG:COG0098", "COG:J", "GO:0003735", "GO:0005737", "GO:0006412", "GO:0015935", "GO:0019843", "KEGG:K02988", "RefSeq:WP_010874539.1", "SO:0001217", "UniParc:UPI0000135061", "UniRef:UniRef100_Q50301", "UniRef:UniRef50_Q50301", "UniRef:UniRef90_Q50301" ], "nt": "ATGACTGATCAAAACCAAAAAGCAAATCAAGGTAACGGCTTGCAAACCACTAACCTCCAAGCCCACGCACAGCGTAAACACAACCTCCGTCCTTCCTCTGAAGGGATTAAGAAAGCCGTGTCCAAAAAGGAAGGTGGTGGTCACAACCGTAACAACCAAAACCGTCGCTTCCAAAAACCGGCTTTCAAGAGTGAGTTTGAAGAACGCATCGTCAAACTAAAGCGCATTTCTAAAACCACTAAGGGTGGACGGAACATGCGGTTTAGTGTGCTGGTCGTAGTTGGTAACCGCAAGGGCAAGATCGGTTATGGGATTGCTAAGGCCTTGGAAGTACCGAATGCGATTAAGAAAGCCATTAAAGCAGCGCACAACTCACTCCACACAATTGAAATCCACAAGGGTTCAATTTACCACGAAGTAATTGGCCGTAGTGGGGCGAGTCGCGTGTTGCTCAAACCCGCTCCTCAAGGTACGGGAATTATTGCTGGTGGTGCCATTCGGGCCATTATTGAGCTCGCTGGTTACAGTGACATTTACACCAAGAACCTCGGTCGCAACACGCCAATTAACATGATCCACGCCACCATGGATGGGATCTTAAAGCAGCTCTCTCCACGTCGGGTAGCGATCTTACGTAACAAAAACCTCAACGAACTTTAG", "aa": "MTDQNQKANQGNGLQTTNLQAHAQRKHNLRPSSEGIKKAVSKKEGGGHNRNNQNRRFQKPAFKSEFEERIVKLKRISKTTKGGRNMRFSVLVVVGNRKGKIGYGIAKALEVPNAIKKAIKAAHNSLHTIEIHKGSIYHEVIGRSGASRVLLKPAPQGTGIIAGGAIRAIIELAGYSDIYTKNLGRNTPINMIHATMDGILKQLSPRRVAILRNKNLNEL", "aa_hexdigest": "02492a98fc2901ada19459181bae0900", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000135061", "ncbi_nrp_id": "WP_010874539.1", "uniref100_id": "UniRef100_Q50301", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000135061", "RefSeq:WP_010874539.1", "UniRef:UniRef100_Q50301" ] }, "ips": { "uniref100_id": "UniRef100_Q50301", "uniref90_id": "UniRef90_Q50301" }, "psc": { "uniref90_id": "UniRef90_Q50301", "gene": "rpsE", "product": "30S ribosomal protein S5", "uniref50_id": "UniRef50_Q50301", "cog_id": "COG0098", "cog_category": "J", "kegg_orthology_id": "K02988", "go_ids": [ "GO:0003735", "GO:0005737", "GO:0006412", "GO:0015935", "GO:0019843" ] }, "pscc": { "uniref50_id": "UniRef50_Q50301", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50301" ], "product": "Small ribosomal subunit protein uS5" }, "genes": [ "rpsE" ], "id": "APKECPOBEN_436", "locus": "GCA002090215_00400" }, { "type": "cds", "sequence": "contig_1", "start": 227042, "stop": 227497, "strand": "+", "frame": 2, "gene": "rplO", "product": "50S ribosomal protein L15", "db_xrefs": [ "COG:COG0200", "COG:J", "GO:0003735", "GO:0006412", "GO:0015934", "GO:0019843", "GO:0022625", "KEGG:K02876", "RefSeq:WP_010874540.1", "SO:0001217", "UniParc:UPI0000133C17", "UniRef:UniRef100_Q50300", "UniRef:UniRef50_P47415", "UniRef:UniRef90_Q50300" ], "nt": "ATGGAACTCAATCAACTCAAAAGTGTCCCCAAAGCGCGAAACCACAAGACCAAAACTTTAGGTCGTGGGCACGGCTCGGGTTTAGGTAAAACATCTGGTCGAGGCCAGAAGGGGCAAAAAGCGCGCAAGTCCGGTTTAACCCGTCCTGGTTTTGAAGGAGGACAAACGCCACTGTACCGTCGTTTACCGAAGTTTGGTAACGCACGCAAGGGCTTTTTAAAGCAGGAGTGGGTGGTGTTAAACCTCAATAAAATTGCTAAATTAAAGCTCGACAAAATTAACCGCGCTAGCTTAATTGAAAAGCAAGTAATTAGCGCAAAGAGCCAATTACCAATTAAACTAATTGGCCACACTAAGCTCGAAAAGCCGTTGCACTTTGAGGTGCACAAGGTTTCCAAGCAAGCTTTGAAAGCGGTTGAAAACGCCAACGGTAGTGTGAAGCTGTTAGAGAAATAA", "aa": "MELNQLKSVPKARNHKTKTLGRGHGSGLGKTSGRGQKGQKARKSGLTRPGFEGGQTPLYRRLPKFGNARKGFLKQEWVVLNLNKIAKLKLDKINRASLIEKQVISAKSQLPIKLIGHTKLEKPLHFEVHKVSKQALKAVENANGSVKLLEK", "aa_hexdigest": "bd3e09912b72db60759ec3a2cb42c77a", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000133C17", "ncbi_nrp_id": "WP_010874540.1", "uniref100_id": "UniRef100_Q50300", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000133C17", "RefSeq:WP_010874540.1", "UniRef:UniRef100_Q50300" ] }, "ips": { "uniref100_id": "UniRef100_Q50300", "uniref90_id": "UniRef90_Q50300" }, "psc": { "uniref90_id": "UniRef90_Q50300", "gene": "rplO", "product": "50S ribosomal protein L15", "uniref50_id": "UniRef50_P47415", "cog_id": "COG0200", "cog_category": "J", "kegg_orthology_id": "K02876", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015934", "GO:0019843", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_P47415", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47415" ], "product": "Large ribosomal subunit protein uL15" }, "genes": [ "rplO" ], "id": "APKECPOBEN_437", "locus": "GCA002090215_00401" }, { "type": "cds", "sequence": "contig_1", "start": 227497, "stop": 228930, "strand": "+", "frame": 1, "gene": "secY", "product": "preprotein translocase subunit SecY", "db_xrefs": [ "COG:COG0201", "COG:U", "GO:0005886", "GO:0006605", "GO:0015031", "GO:0016020", "GO:0043952", "GO:0065002", "RefSeq:WP_015344889.1", "SO:0001217", "UniParc:UPI0002B848D7", "UniRef:UniRef100_UPI0002B848D7", "UniRef:UniRef50_O52351", "UniRef:UniRef90_Q59548" ], "nt": "ATGCAAGCCAAACCAACAACTGCAGTTAAACAAAAACAGAATTTTGGGCAACGTTTGTTCACTTTGTTGCGCAACCGTGACTTCATGATCTCGTTTTTAATAACGGTCGTGCTGTTGGTTTTGTTTCGTGTTTTAGCGATTATTCCGCTCCCCGGCATCCAAGTCAACCAAACCGGGCTAGACCAAAACTCAAACGATTTCTTCTCACTGTTTAACCTACTTGGTGGGGGTGGATTGAACCAACTATCGCTGTTTGCGGTGGGAATTAGTCCGTATATTTCGGCGCAAATTGTTATGCAGCTGTTGTCAACGGACTTAATTCCCCCGTTATCTAAACTGGTGAACAGTGGGGAAGTGGGTCGGCGTAAAATCGAGATGATTACGCGGATTATTACGCTACCGTTTGCGTTGGTGCAATCGTTTGCTGTGATCCAAATTGCCACTAATTCCGGTGGCGGTTCTTCACCTATTACCTTAAAAAACAACGGTTCGGATTTTGTCGCGTTTTACATCATTGCCATGACTGCTGGCACCTACCTGTCGGTCTTTTTAGGTGACACCATTTCCAAAAAGGGAATTGGTAACGGGATCACCCTGTTAATTCTTTCGGGGATTTTAGCCCAGCTACCCGAAGGATTTATAGCTGCTTACAGCGTGTTATCAGGTGTAGTGGTAACCATTAACGCAACCTTAACCACTGCCATTAGCTTCTTCATTTACTTCATGGCGTTTTTGGCATTACTGTTTGCCACTACCTTTATTACCCAAGCGACCCGGAAGATTCCGATTCAACAATCCGGTCAGGGTTTAGTAACCGAATCCTCTGCGTTACCTTACCTCCCAATTAAGGTCAACTCCGCCGGGGTAATTCCGGTGATCTTTGCGTCTTCGATTATGTCAATTCCGGTGACGATTGCCCAGTTCCAACCGCAAACCGAATCACGCTGGTTTGTGGAGGACTATCTTTCACTGTCCAAACCAACTGGCATTGTCCTGTATGGTATCTTGGTGATTCTCTTTAGCTTCTTTTACAGCTACATCCAGATCAATCCAGAACGGTTAGCCAAAAACTTCGAGAAGTCCGGGCGTTTTATCCCGGGTATTCGTCCCGGTAAGGATACTGAGAAACACATTGCCAGAGTACTCGTGCGGATTAACTTTATTGGCGCCCCTTTCCTAACGGTGATTGCCATTATTCCTTACATAGTGTCAGCCTTAATCCACTTACCCAACTCCTTAAGTTTAGGGGGTACGGGGATTATTATCATTGTTACCGCTGTGGTTGAGTTTATGTCAGCACTCCGCTCTGCAGCCACCGCTACTAACTACCAACAACTGCGCCGCAACTTGGCGATTGAAGTGCAAAAAACAGCCCAACAAGACAAGGAGGAACAACTGCGCGCTGAAACCCCTGGTATTGGTAACCTATGGTAA", "aa": "MQAKPTTAVKQKQNFGQRLFTLLRNRDFMISFLITVVLLVLFRVLAIIPLPGIQVNQTGLDQNSNDFFSLFNLLGGGGLNQLSLFAVGISPYISAQIVMQLLSTDLIPPLSKLVNSGEVGRRKIEMITRIITLPFALVQSFAVIQIATNSGGGSSPITLKNNGSDFVAFYIIAMTAGTYLSVFLGDTISKKGIGNGITLLILSGILAQLPEGFIAAYSVLSGVVVTINATLTTAISFFIYFMAFLALLFATTFITQATRKIPIQQSGQGLVTESSALPYLPIKVNSAGVIPVIFASSIMSIPVTIAQFQPQTESRWFVEDYLSLSKPTGIVLYGILVILFSFFYSYIQINPERLAKNFEKSGRFIPGIRPGKDTEKHIARVLVRINFIGAPFLTVIAIIPYIVSALIHLPNSLSLGGTGIIIIVTAVVEFMSALRSAATATNYQQLRRNLAIEVQKTAQQDKEEQLRAETPGIGNLW", "aa_hexdigest": "9bd2f0a009abe82a37cdbdefdac8f92f", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0002B848D7", "ncbi_nrp_id": "WP_015344889.1", "uniref100_id": "UniRef100_UPI0002B848D7", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002B848D7", "RefSeq:WP_015344889.1", "UniRef:UniRef100_UPI0002B848D7" ] }, "ips": { "uniref100_id": "UniRef100_UPI0002B848D7", "uniref90_id": "UniRef90_Q59548" }, "psc": { "uniref90_id": "UniRef90_Q59548", "gene": "secY", "product": "preprotein translocase subunit SecY", "uniref50_id": "UniRef50_O52351", "cog_id": "COG0201", "cog_category": "U", "go_ids": [ "GO:0005886", "GO:0006605", "GO:0015031", "GO:0016020", "GO:0043952", "GO:0065002" ] }, "pscc": { "uniref50_id": "UniRef50_O52351", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_O52351" ], "product": "Protein translocase subunit SecY" }, "genes": [ "secY" ], "id": "APKECPOBEN_438", "locus": "GCA002090215_00402" }, { "type": "cds", "sequence": "contig_1", "start": 228930, "stop": 229571, "strand": "+", "frame": 3, "gene": "adk", "product": "Adenylate kinase", "db_xrefs": [ "COG:COG0563", "COG:F", "EC:2.7.4.3", "GO:0004017", "GO:0005524", "GO:0005737", "GO:0008270", "GO:0043231", "GO:0044209", "SO:0001217", "UniParc:UPI0001DD64E2", "UniRef:UniRef100_Q50299", "UniRef:UniRef50_Q50299", "UniRef:UniRef90_Q50299" ], "nt": "ATGGAAAATAAGTTCTTGTTTTTAGGCGCTCCGGGTGTGGGTAAGGGTACACTAGCTAAGCAAGTAGCCAACACCACCGGATTGTTTCACCTTTCCACTGGTGACATCTTTCGCAGTGTGATGCAAGAGCAGGGTGCGTTGAGTCAAACGCTAGCGCACTATATGAACCAGGGTTTGTATGTCCCTGATGAATTGACTAACCAAACCTTTTGGCACTTTGTCACTACCCACCAAAATGAACTCCACAAGGGCTTTATCCTAGATGGGTATCCCCGTACCTTAAACCAACTCGAGTTTTTGCAAAGCAAGTTGCAATTAGACCAGGTGTTTCACCTTAAGCTAAGTGATCCCCAAGTGCTAGTAGCGCGGATCTTAAACCGTTTGGTTTGTCCTAGTTGTGGTAGTGTATACAACAAGCAGAGCAAACCACCATTGAAGGCCAATCAGTGTGATCGTTGCCATGCTACGTTACAAGCGCGCAATGATGACACGGAAGCGGTAATCTTAAAGCGTTTAACGCTTTACGAAGACACCGTTAAACCCTTAATTGAGGCCTTCACCAAGCAGGGCATTCTCACCGTCATTGAAGCCCAATTACCGCTCGAACAACAGGTCAATTTGGTTCAACAGTTAGTGCATTAA", "aa": "MENKFLFLGAPGVGKGTLAKQVANTTGLFHLSTGDIFRSVMQEQGALSQTLAHYMNQGLYVPDELTNQTFWHFVTTHQNELHKGFILDGYPRTLNQLEFLQSKLQLDQVFHLKLSDPQVLVARILNRLVCPSCGSVYNKQSKPPLKANQCDRCHATLQARNDDTEAVILKRLTLYEDTVKPLIEAFTKQGILTVIEAQLPLEQQVNLVQQLVH", "aa_hexdigest": "fd4a177fd8fd988c3ebaacc33fb1ce32", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD64E2", "uniref100_id": "UniRef100_Q50299", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD64E2", "UniRef:UniRef100_Q50299" ] }, "ips": { "uniref100_id": "UniRef100_Q50299", "uniref90_id": "UniRef90_Q50299" }, "psc": { "uniref90_id": "UniRef90_Q50299", "gene": "adk", "product": "Adenylate kinase", "ec_ids": [ "2.7.4.3" ], "uniref50_id": "UniRef50_Q50299", "cog_id": "COG0563", "cog_category": "F", "go_ids": [ "GO:0005737", "GO:0043231", "GO:0004017", "GO:0005524", "GO:0008270", "GO:0044209" ] }, "pscc": { "uniref50_id": "UniRef50_Q50299", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50299" ], "product": "Adenylate kinase" }, "genes": [ "adk" ], "id": "APKECPOBEN_439", "locus": "GCA002090215_00403" }, { "type": "cds", "sequence": "contig_1", "start": 229571, "stop": 230317, "strand": "+", "frame": 2, "gene": "map", "product": "type I methionyl aminopeptidase", "db_xrefs": [ "COG:COG0024", "COG:J", "EC:3.4.11.18", "GO:0004239", "GO:0005829", "GO:0006508", "GO:0046872", "GO:0070006", "KEGG:K01265", "RefSeq:WP_010874543.1", "SO:0001217", "UniParc:UPI0000125A05", "UniRef:UniRef100_Q11132", "UniRef:UniRef50_P47418", "UniRef:UniRef90_Q11132" ], "nt": "ATGGTTTATTTAAAGTCTGCGCGTGAGGTCGAACAGATCCGCCAAGCATGTAAGATTTTCCAAGAAGCAAAGGCATATTTTACAATTGAAAGGCTACTTGGCAAATCTTTAACAGCAATTGATCAAGCGTTAAAGCAGTTTATTGAGAGCAAAGGTGCAACTTGTGCCTTTCATAAATACCAAAATTTCCCGGGATTTAACTGCCTTTCGTTAAACGAAACGGTAATCCATGGCATAGCGGACAACCGTGTTTTTGGTGTTAAGGATAAACTGACGTTGGATATTGGCATTAACCTTAACGGTTATATTTGCGATGCAGCTTTTACGGTTTTGGGCCCCAAGGCTCCAGAGCCCATGCAAACGCTGTTAGAGGTAACGGAAGCTTGTTTTACTGCTGTGGTGGAGCCTCAACTGCGCCCCAATAACCCCACTGGTAATGTTTCCCATGCCATCCAAACCTACTTTGAATCTAAGGGTTATTACCTTTTAAAACAGTTTGGTGGTCATGGTTGTGGGATTAAGGTGCACGAAGAACCGCTGATTTTGAACTATGGTAAGCCTGATACAGGTACTAAGTTAGAACCAGGGATGGTCTTGTGCATTGAGCCCATGGTAATGACTGACAGTGATGCAATGGTAATGCATAACAACAGCTGGAATGTATTAACCCCTAAAAGCCGTTACAATTGTCATGTAGAACAGATGTATGTGATCACCACAAGTGGATTTGAATGTTTAACTAATTAA", "aa": "MVYLKSAREVEQIRQACKIFQEAKAYFTIERLLGKSLTAIDQALKQFIESKGATCAFHKYQNFPGFNCLSLNETVIHGIADNRVFGVKDKLTLDIGINLNGYICDAAFTVLGPKAPEPMQTLLEVTEACFTAVVEPQLRPNNPTGNVSHAIQTYFESKGYYLLKQFGGHGCGIKVHEEPLILNYGKPDTGTKLEPGMVLCIEPMVMTDSDAMVMHNNSWNVLTPKSRYNCHVEQMYVITTSGFECLTN", "aa_hexdigest": "2f1b193bf84ffb3adf81d5e2d92c6692", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000125A05", "ncbi_nrp_id": "WP_010874543.1", "uniref100_id": "UniRef100_Q11132", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000125A05", "RefSeq:WP_010874543.1", "UniRef:UniRef100_Q11132" ] }, "ips": { "uniref100_id": "UniRef100_Q11132", "uniref90_id": "UniRef90_Q11132" }, "psc": { "uniref90_id": "UniRef90_Q11132", "gene": "map", "product": "type I methionyl aminopeptidase", "ec_ids": [ "3.4.11.18" ], "uniref50_id": "UniRef50_P47418", "cog_id": "COG0024", "cog_category": "J", "kegg_orthology_id": "K01265", "go_ids": [ "GO:0004239", "GO:0005829", "GO:0006508", "GO:0046872", "GO:0070006" ] }, "pscc": { "uniref50_id": "UniRef50_P47418", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47418" ], "product": "Methionine aminopeptidase" }, "genes": [ "map" ], "id": "APKECPOBEN_440", "locus": "GCA002090215_00404" }, { "type": "cds", "sequence": "contig_1", "start": 230317, "stop": 230553, "strand": "+", "frame": 1, "gene": "infA", "product": "translation initiation factor IF-1", "db_xrefs": [ "COG:COG0361", "COG:J", "GO:0003743", "GO:0005829", "GO:0006413", "GO:0019843", "GO:0043022", "KEGG:K02518", "RefSeq:WP_010874544.1", "SO:0001217", "UniParc:UPI000012D263", "UniRef:UniRef100_Q50298", "UniRef:UniRef50_Q50298", "UniRef:UniRef90_Q50298" ], "nt": "ATGCAACCAAAGTTTAATAACCAAGCAAAGCAGGACAAACTCGTCTTAACGGGCAAAATCTTGGAAATCATCCACGGTGACAAGTTTCGCGTGTTGTTGGAAAACAACGTTGAAGTAGATGCTCACTTAGCGGGCAAAATGCGAATGCGCCGGTTACGAATTTTACCGGGCGATTTAGTTGAAGTGGAATTTTCCCCTTATGATTTAAAGTTAGGTCGAATAATAGGTAGAAAATAA", "aa": "MQPKFNNQAKQDKLVLTGKILEIIHGDKFRVLLENNVEVDAHLAGKMRMRRLRILPGDLVEVEFSPYDLKLGRIIGRK", "aa_hexdigest": "95747ef8ca2e0f64bb414f5618436f45", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000012D263", "ncbi_nrp_id": "WP_010874544.1", "uniref100_id": "UniRef100_Q50298", "db_xrefs": [ "SO:0001217", "UniParc:UPI000012D263", "RefSeq:WP_010874544.1", "UniRef:UniRef100_Q50298" ] }, "ips": { "uniref100_id": "UniRef100_Q50298", "uniref90_id": "UniRef90_Q50298" }, "psc": { "uniref90_id": "UniRef90_Q50298", "gene": "infA", "product": "translation initiation factor IF-1", "uniref50_id": "UniRef50_Q50298", "cog_id": "COG0361", "cog_category": "J", "kegg_orthology_id": "K02518", "go_ids": [ "GO:0003743", "GO:0005829", "GO:0006413", "GO:0019843", "GO:0043022" ] }, "pscc": { "uniref50_id": "UniRef50_Q50298", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50298" ], "product": "Translation initiation factor IF-1" }, "genes": [ "infA" ], "id": "APKECPOBEN_441", "locus": "GCA002090215_00405" }, { "type": "cds", "sequence": "contig_1", "start": 230677, "stop": 230934, "strand": "+", "frame": 1, "gene": null, "product": "30S ribosomal protein S13", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A535XWH1" ], "nt": "ATGGCACGGATTTTAGGTATTGACATTCCCAACCAGAAACGCATAGAAATTGCGTTAACCTATATTTTTGGTATAGGGCTTTCGCGCTCCCAAGCCATCTTAAAGCAAGCGAACATTAACCCCGATAAACGGGTCAAAGACTTAACCGAAGAGGAGTTTGTTGCCATTAGGAATGTAGCTAGTGCTTACAAGATTGAGGGTGATTTGCGTCGTGAAATTGCACTCAACATTAAGCATTTGAGTGAAATTGGGGCATGA", "aa": "MARILGIDIPNQKRIEIALTYIFGIGLSRSQAILKQANINPDKRVKDLTEEEFVAIRNVASAYKIEGDLRREIALNIKHLSEIGA", "aa_hexdigest": "7d2df25be7fa43522d3bb68be647b043", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0031B5D25D", "query_cov": 1.0, "subject_cov": 0.9770114942528736, "identity": 0.565, "score": 95.5, "evalue": 4.27e-24, "valid": false, "product": "30S ribosomal protein S13", "uniref50_id": "UniRef50_A0A535XWH1", "kegg_orthology_id": "K02952" }, "pscc": { "uniref50_id": "UniRef50_A0A535XWH1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A535XWH1" ], "product": "30S ribosomal protein S13" }, "genes": [], "id": "APKECPOBEN_442", "locus": "GCA002090215_00406" }, { "type": "cds", "sequence": "contig_1", "start": 231051, "stop": 231161, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTAAGAAGAAAAAGATTAATGTATCTAGTGGAATTATCCACGTATCCTGTTCCCCAAATAACACAATTGTATCCGCTAGTGATCCGGGGGGCAACGTGTTGTGCTGA", "aa": "MAKKKKINVSSGIIHVSCSPNNTIVSASDPGGNVLC", "aa_hexdigest": "6fa3ed66a08dcd96c26be2d606006744", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3670.243200000001, "isoelectric_point": 9.303384208679198 }, "id": "APKECPOBEN_443", "locus": "GCA002090215_00407" }, { "type": "cds", "sequence": "contig_1", "start": 231177, "stop": 231416, "strand": "+", "frame": 3, "gene": "rpsK", "product": "30S ribosomal protein S11", "db_xrefs": [ "COG:COG0100", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904", "KEGG:K02948", "SO:0001217", "UniRef:UniRef50_Q50296", "UniRef:UniRef90_Q50296" ], "nt": "ATGGGCTTTAAGGGATCGCGCAAAAAGACCCCGTACTCAGCCGGCATAGCAGCCGATAAGGTGGCCAAAACCGTCAAGGAAATGGGGATGGCGACCGTTAAGCTCTTTGTCAAGGGTACAGGTCGGGGTAAGGATACCGCCATTCGCAGCTTTGCTAATGCGGGACTTTCTATTACTGAAATTAACGAAAAGACCCCGATTCCCCACAACGGTTGCAAACCACCAAAACGACCACGCTAA", "aa": "MGFKGSRKKTPYSAGIAADKVAKTVKEMGMATVKLFVKGTGRGKDTAIRSFANAGLSITEINEKTPIPHNGCKPPKRPR", "aa_hexdigest": "7f2dd1aa2c4078964274d355c8fc4a0b", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A7Z0ENF1", "query_cov": 1.0, "subject_cov": 0.8061224489795918, "identity": 0.544, "score": 94.7, "evalue": 9.23e-24, "valid": false, "gene": "rpsK", "product": "30S ribosomal protein S11", "uniref50_id": "UniRef50_A0A2H5B013", "kegg_orthology_id": "K02948", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "genes": [ "rpsK" ], "truncated": "5-prime", "pseudogene": { "start": 231051, "stop": 231416, "observations": { "mutation": [ 231159 ], "stop": [ 231159 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q50296", "query_cov": 1.0, "subject_cov": 0.6528925619834711, "identity": 1.0, "score": 161.0, "evalue": 8.04e-50, "gene_start": 1, "gene_end": 79, "reference_start": 43, "reference_end": 121, "reference_sequence": "MAKKKKINVSSGIIHVSCSPNNTIVSASDPGGNVLCWASSGTMGFKGSRKKTPYSAGIAADKVAKTVKEMGMATVKLFVKGTGRGKDTAIRSFANAGLSITEINEKTPIPHNGCKPPKRPR" }, "paralog": false, "identity": 0.9917355371900827, "score": 241.0, "evalue": 9.45e-84, "description": "Internal stop codon at 231159. Nonsense mutation around 231159.", "psc": { "uniref90_id": "UniRef90_Q50296", "gene": "rpsK", "product": "30S ribosomal protein S11", "uniref50_id": "UniRef50_Q50296", "cog_id": "COG0100", "cog_category": "J", "kegg_orthology_id": "K02948", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q50296", "UniRef:UniRef50_Q50296", "COG:COG0100", "COG:J", "KEGG:K02948", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_Q50296", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50296" ], "product": "Small ribosomal subunit protein uS11" } }, "id": "APKECPOBEN_444", "locus": "GCA002090215_00408" }, { "type": "cds", "sequence": "contig_1", "start": 231422, "stop": 231748, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01193.29" ], "nt": "ATGGAAAAGTTTTTAAAGTACGAAATAAAGGTAAATAACGAACAAGCACGCGCTAACCCCAACTACGGCATCTTTGAAGTTGGACCACTCGAATCGGGCTTTGGCATTACCATCGGTAATGCTATGCGCCGGGTGCTGTTGAGCTGTATTCCAGGTGCCAGTGTGTTTGCACTCTCAATTTCGGGTGCCAAGCAAGAGTTTGCTGCTGTGGAAGGGATGAAGGAAGATGTCACTGAAGTGGTGTTAAACTTCAAGCAATTGGTAGTGAAGATTTCTGACCTTCTGTTTGAAGATGGTGAAATGGTCGAACCACCACTAGAACGTTGA", "aa": "MEKFLKYEIKVNNEQARANPNYGIFEVGPLESGFGITIGNAMRRVLLSCIPGASVFALSISGAKQEFAAVEGMKEDVTEVVLNFKQLVVKISDLLFEDGEMVEPPLER", "aa_hexdigest": "a567bbe0c202bfbdc5329a463bb79ed1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01193.29", "name": "RNA_pol_L", "length": 64, "aa_cov": 0.5740740740740741, "hmm_cov": 0.765625, "evalue": 1.7105257217590461e-10, "score": 35.988704681396484, "start": 29, "stop": 90 } ], "seq_stats": { "molecular_weight": 11923.654199999995, "isoelectric_point": 4.7362455368042 }, "id": "APKECPOBEN_445", "locus": "GCA002090215_00409" }, { "type": "cds", "sequence": "contig_1", "start": 231752, "stop": 232405, "strand": "+", "frame": 2, "gene": "rpoA", "product": "DNA-directed RNA polymerase subunit alpha", "db_xrefs": [ "COG:COG0202", "COG:K", "EC:2.7.7.6", "GO:0000428", "GO:0001054", "GO:0001055", "GO:0001056", "GO:0001057", "GO:0001058", "GO:0001066", "GO:0003677", "GO:0003899", "GO:0005737", "GO:0006351", "GO:0046983", "SO:0001217", "UniRef:UniRef50_P47423", "UniRef:UniRef90_Q50295" ], "nt": "TTGTTAACGGTAACAGCTGAGAAAGCGGGACCGGTGTATGCGAAGGACTTGGAGTGCCCAGCTGGCTTTGAAGTGGTCAACAAGGACTTGTACCTGTTCTCGTTGCAAACTGACAAAAAGGTAACAGTTAACGTGTATGTGAAACAAGGACGGGGGTTTGTTACTTTCTTGGAAAACCGTGAAATGATTAATTCTTTGGGGATTATTGCCACTGACTCGAACTTCTCCCCGGTATTACACTGTGGTTATGAAGTACAGGAATTGAAAACTTCCAAGCAAAAGATTACTGATCACTTAACTTTCAAAATTGCTACTAACGGTGCCATATCCGCTGTGGATGCCTTTGCAATGGCTGCAAAGATCTTGATCGAACACCTCAATCCGATTGTTAATGTCAACGAATCGATTAAGGCGTTGAATATTATCCAAGAGAAAGCCGAAGAACGCCGTGTCCGTTCGTTTGCGAAGCAAATTGAAGAGTTGGACTTTACCGTGCGTACCTTTAACTGTTTGAAGCGTAGTGGGATCCACACCTTACAAGAGTTGTTGTCCAAGTCATTAGCTGATATTCGTGAAATCCGTAACCTCGGTAAGAAATCGGAACGCGAAATTATTAAGAAGGTACACGAATTAGGACTCAAATTAAGATCTTAG", "aa": "MLTVTAEKAGPVYAKDLECPAGFEVVNKDLYLFSLQTDKKVTVNVYVKQGRGFVTFLENREMINSLGIIATDSNFSPVLHCGYEVQELKTSKQKITDHLTFKIATNGAISAVDAFAMAAKILIEHLNPIVNVNESIKALNIIQEKAEERRVRSFAKQIEELDFTVRTFNCLKRSGIHTLQELLSKSLADIREIRNLGKKSEREIIKKVHELGLKLRS", "aa_hexdigest": "cbf888f5bf323cea9f11ef7210ca5bfd", "start_type": "TTG", "rbs_motif": null, "genes": [ "rpoA" ], "truncated": "5-prime", "pseudogene": { "start": 231452, "stop": 232405, "observations": { "mutation": [ 231746 ], "stop": [ 231746 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q50295", "query_cov": 1.0, "subject_cov": 0.6636085626911316, "identity": 0.995, "score": 414.0, "evalue": 1.49e-144, "gene_start": 1, "gene_end": 217, "reference_start": 111, "reference_end": 327, "reference_sequence": "MEKFLKYEIKVNNEQARANPNYGIFEVGPLESGFVITIGNAMRRVLLSCIPGASVFALSISGAKQEFAAVEGMKEDVTEVVLNFKQLVVKISDLLFEDGEMVEPPLERWPLLTVTAEKAGPVYAKDLECPAGFEVVNKDLYLFSLQTDKKVTVNVYVKQGRGFVTFLENREMINSLGIIATDSNFSPVLHCGYEVQELKTSKQKITDHLTFKIATNGAISAVDAFAMAAKILIEHLNPIVNVNESIKALNIIQEKAEERRVRSFAKQIEELDFTVRTFNCLKRSGIHTLQELLSKSLADIREIRNLGKKSEREIIKKVHELGLKLRS" }, "paralog": false, "identity": 0.9936908517350158, "score": 607.0, "evalue": 1.11e-222, "description": "Internal stop codon at 231746. Nonsense mutation around 231746.", "psc": { "uniref90_id": "UniRef90_Q50295", "gene": "rpoA", "product": "DNA-directed RNA polymerase subunit alpha", "ec_ids": [ "2.7.7.6" ], "uniref50_id": "UniRef50_P47423", "cog_id": "COG0202", "cog_category": "K", "go_ids": [ "GO:0000428", "GO:0001054", "GO:0001055", "GO:0001056", "GO:0001057", "GO:0001058", "GO:0001066", "GO:0003677", "GO:0003899", "GO:0005737", "GO:0006351", "GO:0046983" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q50295", "EC:2.7.7.6", "UniRef:UniRef50_P47423", "COG:COG0202", "COG:K", "GO:0000428", "GO:0001054", "GO:0001055", "GO:0001056", "GO:0001057", "GO:0001058", "GO:0001066", "GO:0003677", "GO:0003899", "GO:0005737", "GO:0006351", "GO:0046983" ] }, "pscc": { "uniref50_id": "UniRef50_P47423", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47423" ], "product": "DNA-directed RNA polymerase subunit alpha" } }, "id": "APKECPOBEN_446", "locus": "GCA002090215_00410" }, { "type": "cds", "sequence": "contig_1", "start": 232453, "stop": 232782, "strand": "+", "frame": 1, "gene": "rplQ", "product": "50S ribosomal protein L17", "db_xrefs": [ "COG:COG0203", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625", "KEGG:K02879", "SO:0001217", "UniRef:UniRef50_P47424", "UniRef:UniRef90_Q59547" ], "nt": "ATGACCGTGCGCCAACAGGTAAGCGCTGTTTTGGCTTATGGCAAGATTGAAACAACCTTAAAGAAGGCCAAGAACACCCAAAAGCGTTTAGATAAGTTAATTACCTTAGCTAAAGTTGATAACTTTAATAACCGTCGTCAAGTCAAAAAGTGGTTGTTAAACACCAACTTGTTCGATGTTGATCAACTAATGGATCACTTGTTTAGCAAAGTTGCCCCTAAATACGAAAAAACCCCCGGTGGTTACAGCCGTGTTTTAAAATTAGGGCCACGTCGCGGAGATGCCACGGAAATGGCCATCTTGCAACTAACTGATGCTAAATACAAATAA", "aa": "MTVRQQVSAVLAYGKIETTLKKAKNTQKRLDKLITLAKVDNFNNRRQVKKWLLNTNLFDVDQLMDHLFSKVAPKYEKTPGGYSRVLKLGPRRGDATEMAILQLTDAKYK", "aa_hexdigest": "58f9dbd146e31873ccf00c49f2709be5", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_Q59547", "query_cov": 1.0, "subject_cov": 0.8790322580645161, "identity": 1.0, "score": 215.0, "evalue": 1.38e-70, "valid": true, "gene": "rplQ", "product": "50S ribosomal protein L17", "uniref50_id": "UniRef50_P47424", "cog_id": "COG0203", "cog_category": "J", "kegg_orthology_id": "K02879", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_P47424", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47424" ], "product": "Large ribosomal subunit protein bL17" }, "genes": [ "rplQ" ], "id": "APKECPOBEN_447", "locus": "GCA002090215_00411" }, { "type": "cds", "sequence": "contig_1", "start": 232766, "stop": 233581, "strand": "+", "frame": 2, "gene": "ecfA1", "product": "energy-coupling factor transporter ATPase", "db_xrefs": [ "COG:COG1122", "COG:PR", "EC:7.-.-.-", "GO:0005524", "GO:0006810", "GO:0009898", "GO:0016887", "GO:0042626", "GO:0043190", "SO:0001217", "UniRef:UniRef50_P47425", "UniRef:UniRef90_Q50294" ], "nt": "ATGCTAAATACAAATAAACAAGCAGCTGCAAGCTTTCAAGGTGTTTATTTTTCTTACAGCGAATTACCATTAATTCGCAACCTCTCCTTTGATGTTTTCGAAGGGGAGTATGTTTGCATAGTTGGCCATAACGGCTCGGGTAAATCCACCATCTCGAAGCTGTTAACGGGCTTGTTAAAACCACAAGCGGGTACCATCAAAATCTTTGGTAAGACGATTTCGAGTGAAAACGTGACTTACCTACGCAACCACATTGGCATTATCTTCCAAAACCCCGATAACCAGTTTATTGGTATTACCGTGGAAGATGACATTGCTTTTGGACTGGAAAACCGCCGTTATACGCGCGAAAAGATGAAGAGCATCATTGAGGATGTAGCCCAACAAGCGGGAATAACGGAGTTATTGCAAAAAGAACCGCATAACCTTTCGGGTGGGCAAAAGCAACGCGTTGCCATTGCCTCGGTGTTAGCCTTAAACCCAGCCATCATTATCTTTGATGAGTCGACTTCGATGTTGGATCCTAAAGCGAAGCGTACCATTAAGCAGTTCATGGTGGAACTGCGCAACCAGGGCAAGTGTGTTATCTCCATTACCCATGACATGGAAGAGGTTACCAAAGCAGACAAGGTTTTGGTCATGAATGAAGGTCGTTTAATTCGTCAAGGTAAACCCAAGGATGTCTTTAACAGTGCAGCCGAATTGCAAAAAATCCGCTTGGATATTCCCTTTTCTTTAAGCCTTTCCACGAAGGTTAATGGTGTGACTAGTACAATAGACTACCAACAACTAATTGAGGCCATTGGTAAGCTATGA", "aa": "MLNTNKQAAASFQGVYFSYSELPLIRNLSFDVFEGEYVCIVGHNGSGKSTISKLLTGLLKPQAGTIKIFGKTISSENVTYLRNHIGIIFQNPDNQFIGITVEDDIAFGLENRRYTREKMKSIIEDVAQQAGITELLQKEPHNLSGGQKQRVAIASVLALNPAIIIFDESTSMLDPKAKRTIKQFMVELRNQGKCVISITHDMEEVTKADKVLVMNEGRLIRQGKPKDVFNSAAELQKIRLDIPFSLSLSTKVNGVTSTIDYQQLIEAIGKL", "aa_hexdigest": "3c727d3ba5186aa4c199538b80687fe0", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_Q50294", "query_cov": 1.0, "subject_cov": 0.9890510948905109, "identity": 1.0, "score": 525.0, "evalue": 5.27e-188, "valid": true, "gene": "ecfA1", "product": "energy-coupling factor transporter ATPase", "ec_ids": [ "7.-.-.-" ], "uniref50_id": "UniRef50_P47425", "cog_id": "COG1122", "cog_category": "PR", "go_ids": [ "GO:0005524", "GO:0006810", "GO:0009898", "GO:0016887", "GO:0042626", "GO:0043190" ] }, "pscc": { "uniref50_id": "UniRef50_P47425", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47425" ], "product": "Energy-coupling factor transporter ATP-binding protein EcfA1" }, "genes": [ "ecfA1" ], "id": "APKECPOBEN_448", "locus": "GCA002090215_00412" }, { "type": "cds", "sequence": "contig_1", "start": 233578, "stop": 234489, "strand": "+", "frame": 1, "gene": "ecfA2", "product": "energy-coupling factor transporter ATPase", "db_xrefs": [ "COG:COG1122", "COG:PR", "EC:3.6.3.-", "GO:0005524", "GO:0006810", "GO:0009898", "GO:0016887", "GO:0042626", "GO:0043190", "RefSeq:WP_010874551.1", "SO:0001217", "UniParc:UPI00001393F6", "UniRef:UniRef100_Q50293", "UniRef:UniRef50_Q50293", "UniRef:UniRef90_Q50293" ], "nt": "ATGAAAAAAATAGTTGAACTCAAAAACCCCTTAAGGGATGGAGAAATCCTCAGTGTTAGTAACCTTTCGTGCTTTTTTAACGAAAAAACAGTCCATGAAGTCAAGGTAATTGACAACTTTAGCTATACCTTTGCTAAAAATAAGGTCTACTGCATTATTGGTGACAGTGGTAGTGGTAAATCAACCTTAGTCAACCATTTTAATGGTTTAATAAAGCCCGCTTACGGAGATATTTGGGTCAAGGATATTTACATTGGTCAAAAGCAACGCAAAATTAAGGACTTTAAACGCTTGCGCAAAACAATTTCGATTGTCTTTCAGTTTCCCGAATACCAACTGTTTAAGGACACAGTGGAAAAGGACATTATGTTCGGTCCAATTGCTTTGGGTCAATCGAAAAACGAAGCACGCCAAAAGGCAGCTTACTACCTCGAAAAAATGGGGTTAAAGTACACTTTTTTAGAGCGCAATCCGTTTGAGTTGAGCGGGGGGCAAAAGCGCCGTGTAGCGATTGCTGGGATTTTAGCAATTGAACCCGAAGTTTTAATCTTTGATGAACCTACCGCTGGACTAGATCCTGAAGGGGAACGAGAAATGATGCGTTTAATTAAGGATGCTAAAGCTAGTGGTCGCACCGTCTTTATGATTACCCACCAAATGGAAAATGTTTTGGAAGTAGCGGATGAAGTGCTGGTGTTGTCCAAGGGCCAACTCGTTAAATCCGGTGATCCTTACGAAGTTTTTATGGATGAAGCATTTTTGGCACACACCACCATTATTATGCCTCCAGTCATTCAAGTTATCAAAGACCTAATTAACTTAAACCCGAAGTTTAGTTGTTTATTAGACTTTAAACCGCGCAACTTAGACCAGTTGGCGGATGCCATTAACAACACAATAGCTCATGGATAG", "aa": "MKKIVELKNPLRDGEILSVSNLSCFFNEKTVHEVKVIDNFSYTFAKNKVYCIIGDSGSGKSTLVNHFNGLIKPAYGDIWVKDIYIGQKQRKIKDFKRLRKTISIVFQFPEYQLFKDTVEKDIMFGPIALGQSKNEARQKAAYYLEKMGLKYTFLERNPFELSGGQKRRVAIAGILAIEPEVLIFDEPTAGLDPEGEREMMRLIKDAKASGRTVFMITHQMENVLEVADEVLVLSKGQLVKSGDPYEVFMDEAFLAHTTIIMPPVIQVIKDLINLNPKFSCLLDFKPRNLDQLADAINNTIAHG", "aa_hexdigest": "6d515d943aee2ee12155c598a96e60ca", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001393F6", "ncbi_nrp_id": "WP_010874551.1", "uniref100_id": "UniRef100_Q50293", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001393F6", "RefSeq:WP_010874551.1", "UniRef:UniRef100_Q50293" ] }, "ips": { "uniref100_id": "UniRef100_Q50293", "uniref90_id": "UniRef90_Q50293" }, "psc": { "uniref90_id": "UniRef90_Q50293", "gene": "ecfA2", "product": "energy-coupling factor transporter ATPase", "ec_ids": [ "3.6.3.-" ], "uniref50_id": "UniRef50_Q50293", "cog_id": "COG1122", "cog_category": "PR", "go_ids": [ "GO:0005524", "GO:0006810", "GO:0009898", "GO:0016887", "GO:0042626", "GO:0043190" ] }, "pscc": { "uniref50_id": "UniRef50_Q50293", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50293" ], "product": "Energy-coupling factor transporter ATP-binding protein EcfA2" }, "genes": [ "ecfA2" ], "id": "APKECPOBEN_449", "locus": "GCA002090215_00413" }, { "type": "cds", "sequence": "contig_1", "start": 234482, "stop": 234823, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATAGTTTTTTTATTAATGGTTATGTACCACGAGATACCTTTATCCACCGGCTCCACCCGACAACGAAACTGTTAATCTTTTTGCTGTTCGTGATCCTGGTGTTTGTACCCATTGGCTTTGTTTTTCAAAGTGTGATCTTTGTCTTTGCCACAGTTATTTTCTTTGTAGCCAAACTACCCGGACGGTTTTACCTCTCCTCGATTAAGTCAATCTCACTGTTATTCTTACTACTGTTATTTGTCAACTGGTTTACCTTCCGTGACCCCGGGTTCTACATTACCGCTGATCAGGTTAATACAGTAAAACCACACTTTAACGGTAATAACTTTAACTTCTGA", "aa": "MDSFFINGYVPRDTFIHRLHPTTKLLIFLLFVILVFVPIGFVFQSVIFVFATVIFFVAKLPGRFYLSSIKSISLLFLLLLFVNWFTFRDPGFYITADQVNTVKPHFNGNNFNF", "aa_hexdigest": "db2dfa0461e906685a91c52d88b2aa7a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13196.49359999998, "isoelectric_point": 9.868062782287598 }, "id": "APKECPOBEN_450", "locus": "GCA002090215_00414" }, { "type": "cds", "sequence": "contig_1", "start": 234854, "stop": 235327, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTTTCGCAAGTGTTTAACTTTAACCGTGCCAACATGACAGAGTTAAACAAGATTAACTTCTTCTTTAAGGAAACAGCTAATGCTAATGCATACACCAAGGTAACGGGGATTGATAAATTGGCAGAAATGTTGGCTTCAAAGAATCTCTTTAAGTTCAACGGTTCTACCAATGGCATTGATAAAAACAAGATTCTTGGTGCCTTTCTAGATCATAAGATTGCCGTTTACCTCGGTCGTAGTTGGGGTGGTGACTTTAGTGGTTTTGTGATTGACGTAAGTGTAAGTGATAAAACATCTACCTTCACGATTAAACCCTTTTTAGCTAACTCGAACTATGTGTTGACACTGCGTGCCATTATTCTAGCCTTTTACGTTACCCAAAAGATCTTGATCATGATCATTTTGGCAACAGTGTTAACTTCCACTTCAAGCTCGGTTGAGTTGGCATACGGAATTGAACGCTTGCTGTGA", "aa": "MFSQVFNFNRANMTELNKINFFFKETANANAYTKVTGIDKLAEMLASKNLFKFNGSTNGIDKNKILGAFLDHKIAVYLGRSWGGDFSGFVIDVSVSDKTSTFTIKPFLANSNYVLTLRAIILAFYVTQKILIMIILATVLTSTSSSVELAYGIERLL", "aa_hexdigest": "15aba9bee48ef64822d4ee107cc53f6f", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17461.085499999997, "isoelectric_point": 9.486925697326662 }, "id": "APKECPOBEN_451", "locus": "GCA002090215_00415" }, { "type": "cds", "sequence": "contig_1", "start": 235331, "stop": 235786, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAGCTGTTACGGGTTCCAGTCAATGTGTTTGCGATGACAATTGCAATTGCAATTCGCTTTGTCCCTTCTTTATTGTTGGAATCACAACGGATTTTAAATGCCCAAGCATCACGCGGTTTGGACTTTAAGAACGGCAATTTCTTTGTCAAAATGCGTTCATTGTCTTCCTTGGTTGTACCGATGATTTCGATTGCATTCCGCAATGCTGGGGAGTTGGCGAGTGCCATGGAAGCACGTGGTTATGATCCAACTAAGAAACGTACTACCTACCGCAAGTTTAAGATTGATTGGGTTGATGCCACAGCTTTAATTCTCACCGCTCTTTACTTTGTGGTGATTATTTTCTTAACCGTTAAGGGTGCAGTGTTCCTAGATTTAGGGACACCTGAGTGGTTGCTAACCGGCAAGATTAAAGAGCAGGTAGAGCGCTCATTATCAGTTAAAAGTGCCTAG", "aa": "MKLLRVPVNVFAMTIAIAIRFVPSLLLESQRILNAQASRGLDFKNGNFFVKMRSLSSLVVPMISIAFRNAGELASAMEARGYDPTKKRTTYRKFKIDWVDATALILTALYFVVIIFLTVKGAVFLDLGTPEWLLTGKIKEQVERSLSVKSA", "aa_hexdigest": "092427315b7d96369286f153f0d841c0", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16885.89759999999, "isoelectric_point": 10.232824897766111 }, "id": "APKECPOBEN_452", "locus": "GCA002090215_00416" }, { "type": "cds", "sequence": "contig_1", "start": 235941, "stop": 236162, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCATGCCATTAACCAAACCTTTCACGTTGATCTACCTGATAAGTTACCACTCAAACAGTTAATTGCCAAACTAAACCAATTAAACTACCCTGACATTTTTGTAAAGGCCTTAGTTCCAGTAGCACCGCGATTTCACGCGCGGTTTGCAGTAAAAACTAAGGTTTATGAATACCTTATTAACACCCGAGCTTTTAACCCAGCACAGTACCACACCGTGTGA", "aa": "MHAINQTFHVDLPDKLPLKQLIAKLNQLNYPDIFVKALVPVAPRFHARFAVKTKVYEYLINTRAFNPAQYHTV", "aa_hexdigest": "0b4ed275fcc0223680c33b317db5effd", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8488.865399999997, "isoelectric_point": 9.820742988586424 }, "id": "APKECPOBEN_453", "locus": "GCA002090215_00417" }, { "type": "cds", "sequence": "contig_1", "start": 236178, "stop": 236510, "strand": "+", "frame": 3, "gene": "truA", "product": "tRNA pseudouridine(38-40) synthase TruA", "db_xrefs": [ "COG:COG0101", "COG:J", "EC:5.4.99.12", "GO:0001522", "GO:0003723", "GO:0009451", "GO:0009982", "GO:0031119", "GO:0160147", "SO:0001217", "UniRef:UniRef50_P47428", "UniRef:UniRef90_Q50291" ], "nt": "TTGGATTTAGCACAACTTGAAGCGGATACCACGCTCTTTTTAGGCAGGAAGGATTTTTGGAGTTTTAGCACCGCTAGCCAAAAGGACACGCAGCGCACAATTAGTAAAATTATCGTGCAACAAGATCACACTGGTACGGTTAAACTGACCTTTTTTGGCAACGGTTTTTTAAGAAACCAGATTCGAATGATGGTAGCTTCTCTAGTGGCCTTAAACAATCGAACCCTGATGCGAGAAACAGTTCAACAGCTGTTTGATCAACCACAAAAGGGTGGGTGTAAGGTTAAAGCCCCCGCTTGTGGTCTTTATTTAAAAACTGTCTTATATGAATAA", "aa": "MDLAQLEADTTLFLGRKDFWSFSTASQKDTQRTISKIIVQQDHTGTVKLTFFGNGFLRNQIRMMVASLVALNNRTLMRETVQQLFDQPQKGGCKVKAPACGLYLKTVLYE", "aa_hexdigest": "f5672fd11b674d80eb5d9d5e690c2004", "start_type": "TTG", "rbs_motif": null, "genes": [ "truA" ], "truncated": "5-prime", "pseudogene": { "start": 235878, "stop": 236510, "observations": { "mutation": [ 236160 ], "stop": [ 236160 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q50291", "query_cov": 1.0, "subject_cov": 0.45267489711934156, "identity": 0.991, "score": 220.0, "evalue": 7.31e-71, "gene_start": 1, "gene_end": 110, "reference_start": 134, "reference_end": 243, "reference_sequence": "MPRYLAIVAYNGAPFRGWAKQPGYHTVQGQIEQNLALIYQTPINIYGSGRTDKGVHAINQTFHVDLPDKLPLKQLIAKLNQLNYPDIFVKALVPVAPRFHARFAVKTKVYEYLINTRAFNPAQYHTVWQYNHPLDLAQLEADTTLFLGRKDFWSFSTASQKDTQRTISKIIVQQDHTGTVKLTFFGNGFLRNQIRMMVASLVALNNRTLMRETVQQLFDQPQKGGCKVKAPACGLYLKTVLYE" }, "paralog": false, "identity": 0.9952380952380953, "score": 421.0, "evalue": 1.39e-152, "description": "Internal stop codon at 236160. Nonsense mutation around 236160.", "psc": { "uniref90_id": "UniRef90_Q50291", "gene": "truA", "product": "tRNA pseudouridine(38-40) synthase TruA", "ec_ids": [ "5.4.99.12" ], "uniref50_id": "UniRef50_P47428", "cog_id": "COG0101", "cog_category": "J", "go_ids": [ "GO:0001522", "GO:0003723", "GO:0009451", "GO:0009982", "GO:0031119", "GO:0160147" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q50291", "EC:5.4.99.12", "UniRef:UniRef50_P47428", "COG:COG0101", "COG:J", "GO:0001522", "GO:0003723", "GO:0009451", "GO:0009982", "GO:0031119", "GO:0160147" ] }, "pscc": { "uniref50_id": "UniRef50_P47428", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47428" ], "product": "tRNA pseudouridine synthase A" } }, "id": "APKECPOBEN_454", "locus": "GCA002090215_00418" }, { "type": "cds", "sequence": "contig_1", "start": 236707, "stop": 236904, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAGTAACAAGCACGCTGAAAACAGTTTGGACAAAACGATTAACGACGCTTTATTTCAGTACGAAATGTTTAAAAGTGAACATGCCTTGGTGTTTGTTGACTTTGAAAAGAACCTGTTTAAGCACGAAAAGGTGATTCGCGCATACCTCCAAGACCCTGCTTTAAAACAATACCAAAGGGATTTTGAACTAGTATGA", "aa": "MSNKHAENSLDKTINDALFQYEMFKSEHALVFVDFEKNLFKHEKVIRAYLQDPALKQYQRDFELV", "aa_hexdigest": "e91f966ec6cab7e0ec89fe83b197b7ff", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7822.796200000001, "isoelectric_point": 5.83767147064209 }, "id": "APKECPOBEN_455", "locus": "GCA002090215_00419" }, { "type": "cds", "sequence": "contig_1", "start": 236995, "stop": 237147, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTATCTACCGCTGACCTAAACCTCCAACCAGTCGTATACAAGGGTAAAACTTACGTTATTAACGCGGTTAGTGATTATCAAAGTCTCTTGGAAAATAAGGATCGTGGCCTGCGTGAAGCTGCTTACAAAGTGTGGTTGGAAATTTACTGA", "aa": "MLSTADLNLQPVVYKGKTYVINAVSDYQSLLENKDRGLREAAYKVWLEIY", "aa_hexdigest": "3c1ba1fd0c62994acf993281f85ddde7", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5780.5607, "isoelectric_point": 6.059911918640137 }, "id": "APKECPOBEN_456", "locus": "GCA002090215_00420" }, { "type": "cds", "sequence": "contig_1", "start": 237169, "stop": 237525, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAGTTTAGTGGAAAACTACATCCAATTAGAAACCTTTGCTAAGTTAAAGAAACACCCCAATTACATTGCCAAAACAGCTTTTGATGATGAAATTGATGTGGCTTTCATTGACTTTGTTTATGAGCAAGTGGCTAGCTTTGCCCCGACCTTTAAAGCCTTTCAAAGTTTAAGGAAGCAAATTTACAAGCACGTTTTAAAACTAGACAAAGCCCAACCCTGGGACTTGTCGGTACCGTTGTTTAAGGCTAGTGGTGACTACACCATTGAACAAGCCCAAACTGATGCGCTTAAGATATTAGCACCTATGGGTAGTGAATACCTGGAAGTGGTAAAGGAAGCCTTTCGGGAGCGCTGA", "aa": "MSLVENYIQLETFAKLKKHPNYIAKTAFDDEIDVAFIDFVYEQVASFAPTFKAFQSLRKQIYKHVLKLDKAQPWDLSVPLFKASGDYTIEQAQTDALKILAPMGSEYLEVVKEAFRER", "aa_hexdigest": "32012e5235e7afde35b71028d3387805", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13640.518099999988, "isoelectric_point": 5.671246910095214 }, "id": "APKECPOBEN_457", "locus": "GCA002090215_00421" }, { "type": "cds", "sequence": "contig_1", "start": 237610, "stop": 238257, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATTTTGATAAAACCCGCGCTTCATTAAATACCCTAGTCCATGAATTGGGTCACAGTGTCCATTCTTGGTATGCATCTAAGTACCAAACGCAAAACTTGGATCCTACAATTTTCTATGCTGAAATTGCTTCCATTTGTAACGAATTACTGTTGTGTTACCACGACATTATTAACTATGAAAACCGCAATCCGCAGCAGTTAATCCGTAGTTTAATGGAACAAATTAGCCACTTCTTTGGCGCTACCACGAGACAGTTAATGTTCTCTCAGTTTGAACAAGACACCTTAAAGTTAATTCAGCAAAACCAAAAGCCTGACTTTAAGACCCTAGTGGAAATTTATGGTAAGACCGCCATTAAGTACCAAGCTGCTAATGCTGACGCTATTACGAAAAAGCTCAAGCAAACCAAGTACCAAAAGTCATTGGCTTACATTACCTCCATTCCGCATTTTTACGCTGGTAACTTCTATGTTTACAAGTACGCCATTGGTCAAGTTGTGGGTACCTTAGTTGGTAAAAAACTGAGTGCTGGTGACAGTAATATGCTCGCTGCTTACAAGCGCTTCTTGTCTTCAGGCTCCACCTTACCACCACTAGAAACAATTAAATTGTTAGGGATTGACCTTACCCAACCAGAACCGTGA", "aa": "MNFDKTRASLNTLVHELGHSVHSWYASKYQTQNLDPTIFYAEIASICNELLLCYHDIINYENRNPQQLIRSLMEQISHFFGATTRQLMFSQFEQDTLKLIQQNQKPDFKTLVEIYGKTAIKYQAANADAITKKLKQTKYQKSLAYITSIPHFYAGNFYVYKYAIGQVVGTLVGKKLSAGDSNMLAAYKRFLSSGSTLPPLETIKLLGIDLTQPEP", "aa_hexdigest": "9b815534645efd0e635c0cdcc39bc895", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 24490.7961, "isoelectric_point": 9.026621437072755 }, "id": "APKECPOBEN_458", "locus": "GCA002090215_00422" }, { "type": "cds", "sequence": "contig_1", "start": 238338, "stop": 238817, "strand": "+", "frame": 3, "gene": "mte1", "product": "Type II methyltransferase M-MpnI", "db_xrefs": [ "EC:2.1.1.72", "GO:0003677", "GO:0009007", "GO:0032259", "SO:0001217", "UniRef:UniRef50_Q50290", "UniRef:UniRef90_Q50290" ], "nt": "GTGCATTACTTTAACCGCGCCAAAAAGGCAAAGAACAATGAGTTTTATACCTTGTTTGAAGACATTGCCGCTGAAGTGGCATGTTATCCCAATGCCTTTAAGGGTAAGGTAGTGTTGTGTAATTGTAATGATGGTTACCAATCGAACTTCTGGCAGTTTTTTCAAAGTCAGTTTCATGCTTTAGGACTCAAAAAACTAGTTGCGATTGCTTTTAATCCACTTGGCAACAGTTATCAACTCAACTTCGATGGAAAGGAAATAAAGGAACTACCTTTAGCTGGTAATGGCAGCTTTGACAGTGCGGAAGCCATTGTTCTTTTAAAGCAAAGTGACATTGTGGTAACTAATCCACCGTTTAGCTTGTTTCAGGACTTTGTTTGTCTTTTAGCAGAGCATGGCAAACAGTTTTTGGTTTTAGGTCACAATGGTGCTGTCGGTTATAATCAGATCTTTAAGCTGTTTAAGGAAGAACAGTTGTGA", "aa": "MHYFNRAKKAKNNEFYTLFEDIAAEVACYPNAFKGKVVLCNCNDGYQSNFWQFFQSQFHALGLKKLVAIAFNPLGNSYQLNFDGKEIKELPLAGNGSFDSAEAIVLLKQSDIVVTNPPFSLFQDFVCLLAEHGKQFLVLGHNGAVGYNQIFKLFKEEQL", "aa_hexdigest": "53cfc47731aef104e2fba8fda9c411f3", "start_type": "GTG", "rbs_motif": null, "genes": [ "mte1" ], "truncated": "3-prime", "pseudogene": { "start": 238338, "stop": 239120, "observations": { "mutation": [ 238815, 238980, 239085 ], "stop": [ 238815, 238980, 239085 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q50290", "query_cov": 1.0, "subject_cov": 0.49843260188087773, "identity": 1.0, "score": 330.0, "evalue": 1.56e-112, "gene_start": 1, "gene_end": 159, "reference_start": 1, "reference_end": 159, "reference_sequence": "MHYFNRAKKAKNNEFYTLFEDIAAEVACYPNAFKGKVVLCNCNDGYQSNFWQFFQSQFHALGLKKLVAIAFNPLGNSYQLNFDGKEIKELPLAGNGSFDSAEAIVLLKQSDIVVTNPPFSLFQDFVCLLAEHGKQFLVLGHNGAVGYNQIFKLFKEEQLWYGHTVNSSMLFQVQSNFKLYDPKSVNFVKKDGQLFQKVPGISWFTNLKKNQQPAWLKTKSRYQGNEHKYPKFDWYDAIFVSKVKEIPLDWFGYMGVPLTFLNCFNPKQFELIDCLANPYATLDTLKTNAYVRSHHGDVRNVKGKRRYVRVVIKQRQNVI" }, "paralog": false, "identity": 0.9846153846153847, "score": 528.0, "evalue": 1.41e-192, "description": "Internal stop codon at 238815, 238980, 239085. Nonsense mutation around 238815, 238980, 239085.", "psc": { "uniref90_id": "UniRef90_Q50290", "gene": "mte1", "product": "Type II methyltransferase M.MpnI", "ec_ids": [ "2.1.1.72" ], "uniref50_id": "UniRef50_Q50290", "go_ids": [ "GO:0003677", "GO:0009007", "GO:0032259" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q50290", "EC:2.1.1.72", "UniRef:UniRef50_Q50290", "GO:0003677", "GO:0009007", "GO:0032259" ] }, "pscc": { "uniref50_id": "UniRef50_Q50290", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50290" ], "product": "Type II methyltransferase M.MpnI" } }, "id": "APKECPOBEN_459", "locus": "GCA002090215_00423" }, { "type": "cds", "sequence": "contig_1", "start": 238842, "stop": 238982, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGTTCCAAGTGCAGTCTAACTTTAAACTGTATGACCCCAAAAGCGTTAACTTTGTTAAAAAGGATGGTCAACTGTTTCAAAAAGTACCGGGAATTTCCTGGTTTACAAACCTCAAGAAAAACCAACAACCAGCTTGA", "aa": "MLFQVQSNFKLYDPKSVNFVKKDGQLFQKVPGISWFTNLKKNQQPA", "aa_hexdigest": "04aa19e2bef512210a762ba521dde680", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5388.203800000001, "isoelectric_point": 10.000222969055176 }, "id": "APKECPOBEN_460", "locus": "GCA002090215_00424" }, { "type": "cds", "sequence": "contig_1", "start": 239097, "stop": 239297, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGGGTGCCATTAACCTTTCTCAATTGCTTTAATCCCAAACAGTTTGAGCTAATTGATTGTCTAGCCAATCCCTACGCTACCCTAGACACATTAAAAACTAATGCTTATGTGAGATCACACCACGGTGATGTGAGGAATGTGAAGGGTAAGCGAAGGTATGTTAGGGTAGTGATTAAACAACGTCAAAATGTGATTTAA", "aa": "MGVPLTFLNCFNPKQFELIDCLANPYATLDTLKTNAYVRSHHGDVRNVKGKRRYVRVVIKQRQNVI", "aa_hexdigest": "741a38653363bee739e4e54424ad7a9f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7660.864300000004, "isoelectric_point": 10.024140739440917 }, "id": "APKECPOBEN_461", "locus": "GCA002090215_00425" }, { "type": "cds", "sequence": "contig_1", "start": 239391, "stop": 240185, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATTCAAACTATTTTTGGGCAGCAGCTTTTTTGGTGTTGCCACCTTACTTATTGCTTGTGGTACTGGTCGCTTTGATCAAATCGATGATGGCAAAATTAAGCTTGCTGTTGTCACATCCCCTAGTGCCGCTAGTTCACTGCAAACCTTATTAGATCAATACAACAACACTAAAGCACCAGCTGATTATCCAGTGGAAGTAGTTTCACTAGACAGTACTGGGAGCTATACTAAAGGGAAATTTGATACCCAAAAACGGTTAGCTGCTAAAGATAAAAATAACTTTTATAACCTAACCTTTAACTACCCCGACTTAGTTTCATCTTTAGCAATTAACGGAATGGAACTCAACCTTGATGGGGTTAATGTTTCTAACTTCGAACAGTCTTTCTTAGACTTCAACAAGAACATTAGTGGCGTACGCAAACCCGGCATTTACGCCTTACCAGCTACCATGAGTGGTGAAGTTTTGGTTTTAAATGGGCCAGTTCTCCACTACATCTTGAACAGTGCCAAGAAGAAGGATGGTACCGAATCAAAAATTAAAACTGCTCAATTAAAAACAGCTTCAACTCAGGCAGAAGTTAAGGGTACTATGACCATGGCTAGTGATGAACAAACCACAAAGCTTTGGACTAATATTCAAAACGCCGCTAAGGAAAATGCCGCTAACGGTACAACTGAGAAAGCGGAAAAAACAGTTTCCGCTAGTTCGTTGCAATTAAAAAATACGAATAAAACCACCGAAGGTACCTTAAAAATTGGTACTGATGACAACACTAAAAATCTTTGA", "aa": "MKFKLFLGSSFFGVATLLIACGTGRFDQIDDGKIKLAVVTSPSAASSLQTLLDQYNNTKAPADYPVEVVSLDSTGSYTKGKFDTQKRLAAKDKNNFYNLTFNYPDLVSSLAINGMELNLDGVNVSNFEQSFLDFNKNISGVRKPGIYALPATMSGEVLVLNGPVLHYILNSAKKKDGTESKIKTAQLKTASTQAEVKGTMTMASDEQTTKLWTNIQNAAKENAANGTTEKAEKTVSASSLQLKNTNKTTEGTLKIGTDDNTKNL", "aa_hexdigest": "aa128dedb0673c1a480e07338b0834c1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 28490.862500000017, "isoelectric_point": 8.911223030090333 }, "id": "APKECPOBEN_462", "locus": "GCA002090215_00426" }, { "type": "cds", "sequence": "contig_1", "start": 240588, "stop": 241406, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTAACATTAAAAAAGGTCGCGCTGATTTTAGTAACTTCTTTAACCACAATTCAAAAACTTTCCAAAGTCTAAGAGATATTTTCAACAGCTTTAAGCCTTTAATTGACCAAAAGGGTTTAATTAGTAACAAACACTTTGATACTCCAGTGAATAATTACGCTAAATTTCACCAATTGGCATTCTATGTATCATCGACAGCTCGCTTTCCTTACTCGTTTGCCAAAGATAATGTAAAACGCTTAATTATTGGTAAACGCGAATTGGAAGTAAACCCTAAATCAATGTTTGCCATTAAGAAGGAAAACGGCAATAACGGTAACAGCAACCTCTTAGGTAAAGTTGCACTAGATAACAATAAATCTATTGAACTTTACGAAAATAATATTCCTAACGGTAAAACTGATGCCATCTTAATTAAGAACCAAACTTTAATTAGTGCGTTGAAGAATCCAAAACAGACAAAGAGCTCTCAAAGATCTTCTCAGTCTACAAACACCCAAAATGATGCCATTTGTTACTTAGCCTTCAACAGCAAGATTAGACCAGACGATAAAGACATTTTCCTATTAGACAAATTTGGTGAAAAATTTGTCACCGCAATTGTTAACTTTGAAGAAAAAACGGAGGTCAAAATTAATACGCTCCAAGAAAAGGAAGCTGTTGTTTTACCAGCACCACAAAAGTTTAAACCTACTGATCCAAAATCAGTGGCACTTGTTCAAGGTCCAAGTTTAATCGGTATTCACGCTAACGCTAACGAAGACCGTTCAACCATTAAGTTTCTCAACTGGTACCTTAATGCTGAAGTTGATTGA", "aa": "MFNIKKGRADFSNFFNHNSKTFQSLRDIFNSFKPLIDQKGLISNKHFDTPVNNYAKFHQLAFYVSSTARFPYSFAKDNVKRLIIGKRELEVNPKSMFAIKKENGNNGNSNLLGKVALDNNKSIELYENNIPNGKTDAILIKNQTLISALKNPKQTKSSQRSSQSTNTQNDAICYLAFNSKIRPDDKDIFLLDKFGEKFVTAIVNFEEKTEVKINTLQEKEAVVLPAPQKFKPTDPKSVALVQGPSLIGIHANANEDRSTIKFLNWYLNAEVD", "aa_hexdigest": "25d33800fb4d75335a085a3d12610ffb", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 30814.648000000023, "isoelectric_point": 9.520384788513184 }, "id": "APKECPOBEN_463", "locus": "GCA002090215_00427" }, { "type": "cds", "sequence": "contig_1", "start": 241464, "stop": 241826, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCGTTTAAAAAAGTATTAGAAAAATCCAAGCAAAACATAAAAGCGACCTCTAAGGAAGGAGAGCAAAATCAAGGCAAGAAAGGTGATGGTGCACAGAACCAAGGTAAGAAGGGCGATGGAGCTCAAAATGGTAAGAATGATAAAGCAAAGCACAATGGTTTTGTCGATATTGCCGTCAGCCAATTTTTAAACGGTTCTGTCGCTAAGTTTGTGGAACCAGCTGACTTTTTAGGTATTCGCTTACGTGATGCCGTTAAGAGTACCTTTAACGCTGCCATTAACAACACCTCAATTAACTTTGACAAGTTCATTCAGTACATTAACGATTCTTTAGGTAGCGACTTTAAGCAAAGACAGTAG", "aa": "MPFKKVLEKSKQNIKATSKEGEQNQGKKGDGAQNQGKKGDGAQNGKNDKAKHNGFVDIAVSQFLNGSVAKFVEPADFLGIRLRDAVKSTFNAAINNTSINFDKFIQYINDSLGSDFKQRQ", "aa_hexdigest": "7483ec5773002d78fc25461d4bb3f3d0", "start_type": "TTG", "rbs_motif": "AAAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13166.572299999983, "isoelectric_point": 9.697608375549319 }, "id": "APKECPOBEN_464", "locus": "GCA002090215_00428" }, { "type": "cds", "sequence": "contig_1", "start": 241956, "stop": 242756, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATTTAAGTATGGTGCCATTGTTTTCAGTGGTCTTTTAGGAGTCTCTGCCATTTTAGCTGCTTGTGGTACACGCGGTAAATTTGACCAAATTGATGATGGCAAAATTAAGCTAGCATCTTCGTTAACTTCCAAAGGTGCCGCTAATGCTTTACAAGCAATTGTGAAGAAGTATAACGAGGTTAAAAAGCCTGGTGATTACCCAATTGAAATAACCCAAATTGCGGGTGGTTACGACCAAGCACGCGTTGACTTACAATCACGGGTTGGTGTTAAGGATAAAACCAATTTTTACAACCTCATTTTGAACTATCCCGATGTAGTTTCAGTGCTAGCACGTAACCAAATGGAACTGCCATTTGATGGTGTTGATGTTTCTAAAATTTCACCTAACTTTTTAAATTTTAACGAACGTATTAGCGGAGTTTCTAAAAAGGCTAACTATGCCATTCCGGTCTCTGTTTCGACTGATATTTTAGTGCTCAATGGACCAGTTTTGCACTACATTTTAAACAGTGCTAAAGGTGAAAGTAAGGGCGCACAAAAAGATAACAAGAGTGCTGAAGTTCAAAGAAAAAGTACAGGTCAAAAGACCGTTACCCAACCTTTAACTATTGCAACTGATTCTGCTACAAACGGTCTTTGGAAAAAGATTGAAGATGCTGCAAAAGTCAACGGTAAAAAGAAAGAAGAGAAAAAGAGTACACGCAGTAAAAGAGCCACAGAAGGCACACAAACCACCAAAGAAAATACGGGTGGTGATGCTGCAACAAGTGATACCAAAATTAAAGAATCTTGA", "aa": "MKFKYGAIVFSGLLGVSAILAACGTRGKFDQIDDGKIKLASSLTSKGAANALQAIVKKYNEVKKPGDYPIEITQIAGGYDQARVDLQSRVGVKDKTNFYNLILNYPDVVSVLARNQMELPFDGVDVSKISPNFLNFNERISGVSKKANYAIPVSVSTDILVLNGPVLHYILNSAKGESKGAQKDNKSAEVQRKSTGQKTVTQPLTIATDSATNGLWKKIEDAAKVNGKKKEEKKSTRSKRATEGTQTTKENTGGDAATSDTKIKES", "aa_hexdigest": "3720b466520c4994075f19e1a0f1c1e3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 28665.232800000027, "isoelectric_point": 9.57415142059326 }, "id": "APKECPOBEN_465", "locus": "GCA002090215_00429" }, { "type": "cds", "sequence": "contig_1", "start": 242916, "stop": 244010, "strand": "+", "frame": 3, "gene": null, "product": "P68 family surface lipoprotein", "db_xrefs": [ "GO:0005886", "GO:0009897", "SO:0001217", "UniRef:UniRef50_P75280", "UniRef:UniRef90_Q50288" ], "nt": "GTGTTAGGTGTTGATAATTCACCTAATGCTTTACTATCTTCGGTGTTTGCTGCTGGTAACAGTGACTATAACAACTTCTTTTATAAGGTGCAAAATGGTCGGGCTGACTTTAGCAACTTTAACAATAAAGGCAGTTCTTATAAAAACCTTAAAAATGTCTTTAACGACTATAAGAACCTAATTGCACAAAATGGTCTTTATGTAAATAAAGGCGGCTCTTACTCTTCAAACTTCCAAAAGTTTCACCAGTTAGCTTACAGCATTTCCTCTACTTCTGGTTTTGCTTATTCGTTTGCTGGACAAAATTCCAAACGTTTTAAATTTACTGATGATGGTACTTTTGTTGAGTATCCTAGTTATACAACTGAAGTTAATGCACCAGAAAGTAATAATGGCAATGATGGAAAGCAACAAGGTCAAAGTGATCAAGGAAACCTTTTAGGAACTTTTGAAGTTGTAGATAAAAGTACTAGTGATATAAAGGTGAAACCTAAAACTCAAGCGGAATCAAAGAAAAGTAGTGATAGTAAACAAACTGCAAACACAGGCAAAGGCAGTAATTCAAAACAACAAACTCCGAAGAAAACTATTTCGCTTTATAAAACCAAAATTCCTCAAGATAAAACAGAAAACGTTGACGCTTTTTTAGTTACTGATTCAGAATTAATTAGCAAACTTGAAAAAGCTAAAAATAAAAAGGAAGAAACTAAAGCTTCAGGTAAATCTGCATCTGCACGTGTGGCAGTTCAAGCAACTCAAAAGAAACAGTCTAACGAAAAACAGATAGTTGGTTACACTACTACATCCGCATTAAGCGAAGACGGCAAACACATTTTTAAATTGGGTAAACTCAACAGCGAGAACTATGAGCGCAAAATCATTGTTGGTGCTACTGTAGAAACACTAGAACAATCCACTACCTTGCAAAGTGAAGAAGCAATTGTTTTAGCAGCACCTGGTAAGTACAAGGATAGTGATCAAAAGAGAGTTATGATCACCCAAGGTCCTAACCTAATAGGGGTTCATGCTAATACTAAAGAAAATGAAGAGACTAAGAAATTTGTTAACTGGTTTTTAAATAAAACCGAAAGTTGA", "aa": "MLGVDNSPNALLSSVFAAGNSDYNNFFYKVQNGRADFSNFNNKGSSYKNLKNVFNDYKNLIAQNGLYVNKGGSYSSNFQKFHQLAYSISSTSGFAYSFAGQNSKRFKFTDDGTFVEYPSYTTEVNAPESNNGNDGKQQGQSDQGNLLGTFEVVDKSTSDIKVKPKTQAESKKSSDSKQTANTGKGSNSKQQTPKKTISLYKTKIPQDKTENVDAFLVTDSELISKLEKAKNKKEETKASGKSASARVAVQATQKKQSNEKQIVGYTTTSALSEDGKHIFKLGKLNSENYERKIIVGATVETLEQSTTLQSEEAIVLAAPGKYKDSDQKRVMITQGPNLIGVHANTKENEETKKFVNWFLNKTES", "aa_hexdigest": "63fbf8a89ca46104fe00074471731e11", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 242616, "stop": 244313, "observations": { "mutation": [ 242754, 242823, 244008 ], "stop": [ 242754, 242823, 244008 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q50288", "query_cov": 1.0, "subject_cov": 0.45614035087719296, "identity": 0.997, "score": 679.0, "evalue": 2.32e-239, "gene_start": 1, "gene_end": 364, "reference_start": 321, "reference_end": 684, "reference_sequence": "MKFKYGAIVFSGLLGVSAILAACGTRGKFDQIDDGKIKLASSLTSKGAANALQAIVKKYNEVKKPGDYPIEITQIAGGYDQARVDLQSRVGVKDKTNFYNLILNYPDVVSVLARNQMELPFDGVDVSKISPNFLNFNERISGVSKKANYAIPVSVSTDILVLNGPVLHYILNSAKGESKGAQKDNKSAEVQRKSTGQKTVTQPLTIATDSATNGLWKKIEDAAKVNGKKKEEKKSTRSKRATEGTQTTKENTGGDAATSDTKIKESWGAYQEVEGGLKGYQFKAIVFENWHDLIDFSTRVAKSFSKVKDNSNKKGNEIQGVLGVDNSPNALLSSVFAAGNSDYNNFFYKVQNGRADFSNFNNKGSSYKNLKNVFNDYKNLIAQNGLYVNKGGSYSSNFQKFHQLAYSISSTSGFAYSFAGQNSKRFKFTDDGTFVEYPSYTTEVNAPESNNGNDGKQQGQSDQGNLLGTFEVVDKSTSDIKVKPKTQAESKKSSDSKQTANTGKGSNSKQQTPKKTISLYKTKIPQDKTENVDAFLVTDSELISKLEKAKNKKEETKASGKSASARVAVQATQKKQSNEKQIVGYTTTSALSEDGKHIFKLGKLNSENYERKIIVGATVETLEQSTTLQSEEAIVLAAPGKYKDSDQKRVMITQGPNLIGVHANTKENEETKKFVNWFLNKTESWEVKGNGKDSQTTKSLTPAQYFAESASYILPLKETVEKDHKEQTNKNTYVAKALEMLKEVSENKSVSYSDPSDFRSGRFRDALGANFNATINSKANFEKFFQGFKAALGSDFDK" }, "paralog": false, "identity": 0.9946902654867257, "score": 1100.0, "evalue": 0.0, "description": "Internal stop codon at 242754, 242823, 244008. Nonsense mutation around 242754, 242823, 244008.", "psc": { "uniref90_id": "UniRef90_Q50288", "product": "P68 family surface lipoprotein", "uniref50_id": "UniRef50_P75280", "go_ids": [ "GO:0005886", "GO:0009897" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q50288", "UniRef:UniRef50_P75280", "GO:0005886", "GO:0009897" ] }, "pscc": { "uniref50_id": "UniRef50_P75280", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75280" ], "product": "Uncharacterized lipoprotein MPN_506" } }, "id": "APKECPOBEN_466", "locus": "GCA002090215_00430" }, { "type": "cds", "sequence": "contig_1", "start": 244095, "stop": 244352, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCGCTAAAGGAAACTGTTGAAAAAGATCATAAAGAACAAACTAATAAAAACACCTATGTTGCCAAAGCTTTAGAAATGCTCAAGGAAGTTAGTGAAAACAAATCAGTCAGCTATAGTGATCCGAGTGATTTTAGAAGTGGTAGATTCCGTGATGCACTTGGTGCTAACTTTAATGCCACTATTAATTCAAAAGCAAATTTTGAAAAGTTTTTCCAAGGTTTTAAAGCTGCATTAGGCTCTGATTTTGATAAATAG", "aa": "MPLKETVEKDHKEQTNKNTYVAKALEMLKEVSENKSVSYSDPSDFRSGRFRDALGANFNATINSKANFEKFFQGFKAALGSDFDK", "aa_hexdigest": "aca2633dd13680634e95ce37c493b8f6", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9616.597199999993, "isoelectric_point": 7.948774528503418 }, "id": "APKECPOBEN_467", "locus": "GCA002090215_00431" }, { "type": "cds", "sequence": "contig_1", "start": 244457, "stop": 244636, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01420.25" ], "nt": "GTGGAGATTAAAACTTACAAAATCAAAGATATTTGTGATATTACACGCGGTAGAGTTATCAGCAAATTGGACATTAAAAAAGATCCAGGAGTTTTTCCTGTTTATTCGGCAGCTACTAATAACGATGGGGAGTTTGGTCGAATAAATTCTTATGACTTTGATGGTGAATATGTTACTTGA", "aa": "MEIKTYKIKDICDITRGRVISKLDIKKDPGVFPVYSAATNNDGEFGRINSYDFDGEYVT", "aa_hexdigest": "5e30dbd3b8714ccd24b8094a741d69d4", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01420.25", "name": "Methylase_S", "length": 61, "aa_cov": 0.9661016949152542, "hmm_cov": 0.9836065573770492, "evalue": 1.6454013143063861e-10, "score": 36.86839294433594, "start": 3, "stop": 59 } ], "seq_stats": { "molecular_weight": 6708.495900000002, "isoelectric_point": 5.161514854431151 }, "id": "APKECPOBEN_468", "locus": "GCA002090215_00432" }, { "type": "cds", "sequence": "contig_1", "start": 244617, "stop": 244712, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTGAATATGTTACTTGAACAGCTGACGGTTATGGAGGGGCTGTTTTTTATCGTAATGGTAAATTTAGCATCACAAATCTTTGTGGTCTTTTGA", "aa": "MVNMLLEQLTVMEGLFFIVMVNLASQIFVVF", "aa_hexdigest": "4165f2c157215872847f65036bab0f87", "start_type": "ATG", "rbs_motif": "AATAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3577.3857, "isoelectric_point": 4.0500284194946286 }, "id": "APKECPOBEN_469", "locus": "GCA002090215_00433" }, { "type": "cds", "sequence": "contig_1", "start": 244757, "stop": 245167, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01420.25" ], "nt": "TTGAAACTAGAAGCACCGAAATTCACAAACCGTGTTTTTAAAAATAGACCTAAGTTAACACATAAAACAATGGCCGAAATTCCCATTGATTTCCCACCCTTGAAAATTCAAGAGAAAATTGCTACTATCTTAGATACTTTTACCGAGCTAAGGGCGCGCAAGAAGCAGTATGCCTTCTATCGCGATTATCTGCTTAATCAAGAAAATATCCGAAAAATTTATGGAGCAAATATACCTTTCGAAACTTTTCAAGTAAAGGATATTTGTGAAATTAGAAGAGGTCGAGCGATCACTAAAGCGTATATTAGGAATAATCCTGGAGAAAATCCAGTTTATTCCGCAGCTACCACAAATGATGGTGAGTTAGGACACATTAAAGATTGCGACTTTGATGGTGAATACATCACTTGA", "aa": "MKLEAPKFTNRVFKNRPKLTHKTMAEIPIDFPPLKIQEKIATILDTFTELRARKKQYAFYRDYLLNQENIRKIYGANIPFETFQVKDICEIRRGRAITKAYIRNNPGENPVYSAATTNDGELGHIKDCDFDGEYIT", "aa_hexdigest": "eb0918893f28262e662f75db13ae20e7", "start_type": "TTG", "rbs_motif": "AAAA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01420.25", "name": "Methylase_S", "length": 62, "aa_cov": 0.4264705882352941, "hmm_cov": 0.9838709677419355, "evalue": 5.452314680643036e-21, "score": 70.96626281738281, "start": 79, "stop": 136 } ], "seq_stats": { "molecular_weight": 15876.039599999982, "isoelectric_point": 9.204489707946777 }, "id": "APKECPOBEN_470", "locus": "GCA002090215_00434" }, { "type": "cds", "sequence": "contig_1", "start": 245354, "stop": 245521, "strand": "+", "frame": 2, "gene": null, "product": "restriction endonuclease subunit S", "db_xrefs": [ "EC:3.1.21.3", "KEGG:K01154", "SO:0001217", "UniRef:UniRef50_UPI00190F3D87", "UniRef:UniRef90_UPI00190F3D87" ], "nt": "ATGGCTGAAATCGAACTCTCTTTCCCACCTCTAGAAATCCAAGAAAAGATTGCCGATATTCTCTTTGCTTTTGAAAAACTTTGCAATGATTTAGTAGAAGGTATTCCTGCCGAAATTGAATTGCGTAAAAAGCAATTGGATTACTATCAGAACTTCTTATTTAATTGA", "aa": "MAEIELSFPPLEIQEKIADILFAFEKLCNDLVEGIPAEIELRKKQLDYYQNFLFN", "aa_hexdigest": "bbbc1991845ad67ac2a240d1c026608d", "start_type": "ATG", "rbs_motif": "AAAA", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 245207, "stop": 245566, "observations": { "mutation": [ 245519, 245540 ], "stop": [ 245519, 245540 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00190F3D87", "query_cov": 1.0, "subject_cov": 0.46218487394957986, "identity": 1.0, "score": 114.0, "evalue": 1.06e-31, "gene_start": 1, "gene_end": 55, "reference_start": 50, "reference_end": 104, "reference_sequence": "GKCNASQDCGVLKVKNKKICTKFLSFLLKIEAPKFVHNLASRPKLSQKVMAEIELSFPPLEIQEKIADILFAFEKLCNDLVEGIPAEIELRKKQLDYYQNFLFNWVQEQKKNSLSTNLN" }, "paralog": false, "identity": 0.8907563025210085, "score": 207.0, "evalue": 8.7e-71, "description": "Internal stop codon at 245519, 245540. Nonsense mutation around 245519, 245540.", "psc": { "uniref90_id": "UniRef90_UPI00190F3D87", "product": "restriction endonuclease subunit S", "ec_ids": [ "3.1.21.3" ], "uniref50_id": "UniRef50_UPI00190F3D87", "kegg_orthology_id": "K01154", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00190F3D87", "EC:3.1.21.3", "UniRef:UniRef50_UPI00190F3D87", "KEGG:K01154" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00190F3D87", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00190F3D87" ], "product": "restriction endonuclease subunit S" } }, "id": "APKECPOBEN_471", "locus": "GCA002090215_00435" }, { "type": "cds", "sequence": "contig_1", "start": 245572, "stop": 245997, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_308010181.1", "SO:0001217", "UniParc:UPI0027E159D2", "UniRef:UniRef100_UPI0027E159D2", "UniRef:UniRef50_A0AB38W7Y3", "UniRef:UniRef90_UPI0027E159D2" ], "nt": "GTGGTTTTCGTTGCCTTGGGTGTTATTCCCAGTAGTCTTATCATTCCTTCACCCCGCCCCCGTTCAGGGCTGGAGGTTGGCTTTGTCGAGGGTGGATCATTTGGAGCCGCCTGTAGTCGTCGTCTGACTGCCACCGAGCGTGGCTTGGACGTTGGCTTTGAGACCAACGCCGACATTCGAGAACGTCGGGAGAAAGGAACGCGAACCACTTGTGTTCGTACTAGTTCCGCTGGACGACCCCGCGCCGGTTTGAGGCGCGGTTATATCATCCACGCCCCGGTGGTTATTGCCATACCTGTACCCATTCCCAATCCAGGGAATATAATCCAACACCTGGTCGAGCACCGACCCCGCGAGGTTGAACCCGGTGGAGAAGCGGGTGAGCGTGGGGGAGAAGTGGGATCAGTTTGTGGTTTGCTGAACTAA", "aa": "MVFVALGVIPSSLIIPSPRPRSGLEVGFVEGGSFGAACSRRLTATERGLDVGFETNADIRERREKGTRTTCVRTSSAGRPRAGLRRGYIIHAPVVIAIPVPIPNPGNIIQHLVEHRPREVEPGGEAGERGGEVGSVCGLLN", "aa_hexdigest": "90cc5db1e0963df56dd341c84f152ab9", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0027E159D2", "ncbi_nrp_id": "WP_308010181.1", "uniref100_id": "UniRef100_UPI0027E159D2", "db_xrefs": [ "SO:0001217", "UniParc:UPI0027E159D2", "RefSeq:WP_308010181.1", "UniRef:UniRef100_UPI0027E159D2" ] }, "ips": { "uniref100_id": "UniRef100_UPI0027E159D2", "uniref90_id": "UniRef90_UPI0027E159D2" }, "psc": { "uniref90_id": "UniRef90_UPI0027E159D2", "uniref50_id": "UniRef50_A0AB38W7Y3" }, "pscc": { "uniref50_id": "UniRef50_A0AB38W7Y3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W7Y3" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14928.934299999983, "isoelectric_point": 9.585820198059082 }, "id": "APKECPOBEN_472", "locus": "GCA002090215_00436" }, { "type": "cds", "sequence": "contig_1", "start": 246022, "stop": 246189, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGCAGTCAAAACCAGGGTTCTACAACAACTACCTCAGCGGGCAATCCCGATTCTTTAGTTACTGATAAAGTTGATCAAAAAGGTCAGGTGCAAACAAGTGGTCAAAATTTAAGTGATACCAACTACACCAACCTCCCCCCCAACCTCACCCCCACATCCGACTGA", "aa": "MGSQNQGSTTTTSAGNPDSLVTDKVDQKGQVQTSGQNLSDTNYTNLPPNLTPTSD", "aa_hexdigest": "098e3a4732806bbe0fbb790231df2247", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5712.955600000001, "isoelectric_point": 4.128068351745605 }, "id": "APKECPOBEN_473", "locus": "GCA002090215_00437" }, { "type": "cds", "sequence": "contig_1", "start": 246271, "stop": 246552, "strand": "+", "frame": 1, "gene": null, "product": "Adhesin P1-like protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB33HTF6", "UniRef:UniRef90_A0AB33HTF6" ], "nt": "GTGTTGGTTAATAAGAGTGGCGAAAATAACGAAAAATTCCAAGCCACCGACCAAAAATGGTCCTACACCGAATTAAAGTCGGATCAAACCAAGCTCAACCTCCCCGCTTATGGTGAGGTGAATGGGCTGTTGAATCCGGCGTTGGTGGAAACCTATTTTGGGACGACGCGTACGTCTAGCACTGCGAACCAAAACAGTACAACCGTCCCCGGTATCGGTTTTAAAATTCCCGAACAAAATAATGATTCAAAGGCTGTGCTGATCACCCCCGGGTTGGCTTGA", "aa": "MLVNKSGENNEKFQATDQKWSYTELKSDQTKLNLPAYGEVNGLLNPALVETYFGTTRTSSTANQNSTTVPGIGFKIPEQNNDSKAVLITPGLA", "aa_hexdigest": "94cb0503eb531ec574d7f6e43d4c288d", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 246199, "stop": 246843, "observations": { "insertion": [ 246724 ], "deletion": [ 246628 ], "mutation": [ 246550 ], "stop": [ 246550, 246694 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB33HTF6", "query_cov": 1.010752688172043, "subject_cov": 0.42727272727272725, "identity": 0.904, "score": 164.0, "evalue": 2.61e-49, "gene_start": 1, "gene_end": 93, "reference_start": 25, "reference_end": 118, "reference_sequence": "MSFTNKNNAQRVQLFLRGLLGSIPVLVNKSGQDDNSKFNSTDQKWSYTELKSDQTKLNLPAYGEVNGLLNPALVETYFGTTLTSSTANQNSTTVPSIGFKIPEQNNDSKAVLITPGLAWTPQDVGNLVVSGTSLSFQLGGWLVTVHGLYQTPRGLFGPPVKWPGCQWQRPKGVNLGQAALSGISWQLGQPVGTGRECVGFEGGVGGSSSQRGVRVASSYK" }, "paralog": false, "identity": 0.8093023255813954, "score": 326.0, "evalue": 1.74e-115, "description": "Internal stop codon at 246550, 246694. Frameshift due to insertion around 246724. Frameshift due to deletion around 246628. Nonsense mutation around 246550.", "psc": { "uniref90_id": "UniRef90_A0AB33HTF6", "product": "Adhesin P1 homolog", "uniref50_id": "UniRef50_A0AB33HTF6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB33HTF6", "UniRef:UniRef50_A0AB33HTF6" ] }, "pscc": { "uniref50_id": "UniRef50_A0AB33HTF6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB33HTF6" ], "product": "Adhesin P1 homolog" } }, "id": "APKECPOBEN_474", "locus": "GCA002090215_00438" }, { "type": "cds", "sequence": "contig_1", "start": 246776, "stop": 247036, "strand": "+", "frame": 2, "gene": null, "product": "Gt9_orf116a protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50285", "UniRef:UniRef50_UPI0034DD7934", "UniRef:UniRef90_Q50285", "UniRef:UniRef90_UPI0034DD7934" ], "nt": "GTGGAGAGTGTGTGGGATTTGAAGGGGGTGTGGGCGGATCAAGCTCAGTCCGACTCGCAAGGATCTACCACCACCGCAACAGGGGCTACCTTACCGGAGCACCCGAATGCTTTGGCCTTTCAGGTGAGTGTGGTGGAAGCGAGTGCTTACAAGCCAAACACGAGCTCCGGCCAAACCCAATCCACTAACAGTTCCCCCTACCTGCACTTGGTGAAGCCTAAGAAAGTTACCCAATCCGACAAAGTTAGACGACGATCTTAA", "aa": "MESVWDLKGVWADQAQSDSQGSTTTATGATLPEHPNALAFQVSVVEASAYKPNTSSGQTQSTNSSPYLHLVKPKKVTQSDKVRRRS", "aa_hexdigest": "7eea8784b8ef1d346e82266d66f44f25", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0034DD7934", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.988, "score": 162.0, "evalue": 1.81e-50, "valid": true, "uniref50_id": "UniRef50_UPI0034DD7934" }, "pscc": { "uniref50_id": "UniRef50_UPI0034DD7934", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0034DD7934" ] }, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 246686, "stop": 247036, "observations": { "mutation": [ 246734 ], "stop": [ 246734 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q50285", "query_cov": 1.0, "subject_cov": 0.7413793103448276, "identity": 0.988, "score": 163.0, "evalue": 1.59e-50, "gene_start": 1, "gene_end": 86, "reference_start": 31, "reference_end": 116, "reference_sequence": "MQVMRRSAPSFGPPRPWAAFRGSWVNRLGRVESVWDLKGVWADQAQSDSQGSTTTATGATLPEHPNALAFQVSVVEASAYKPNTSSGQTQSTNSSPYLHLVKPKKVTQSDKVRRRS" }, "paralog": false, "identity": 0.9913793103448276, "score": 232.0, "evalue": 4.5e-80, "description": "Internal stop codon at 246734. Nonsense mutation around 246734.", "psc": { "uniref90_id": "UniRef90_Q50285", "product": "Gt9_orf116a protein", "uniref50_id": "UniRef50_Q50285", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q50285", "UniRef:UniRef50_Q50285" ] }, "pscc": { "uniref50_id": "UniRef50_Q50285", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50285" ], "product": "Gt9_orf116a protein" } }, "id": "APKECPOBEN_475", "locus": "GCA002090215_00439" }, { "type": "cds", "sequence": "contig_1", "start": 247071, "stop": 247265, "strand": "+", "frame": 3, "gene": null, "product": "Putative adhesin P1-like protein MPN_203", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50284", "UniRef:UniRef50_UPI0027E1A8D0", "UniRef:UniRef90_Q50284", "UniRef:UniRef90_UPI0027E1A8D0" ], "nt": "ATGCTCAAATCATTTGGTACAGAAAATTTCACCCAACCCCAACCCCAACCCCAAGCCCTCAAAACAACGACACCGGTATTTGGGACGAGTAGTGGTAACCTCGGTAGTGTGCTTAGTGGTGGGGGGTACCACGCAGGGCTCAAGCACCACCAATCAACTGTCACCCGTTCAACGGGTGAGTGGGTGGACCGCTAA", "aa": "MLKSFGTENFTQPQPQPQALKTTTPVFGTSSGNLGSVLSGGGYHAGLKHHQSTVTRSTGEWVDR", "aa_hexdigest": "a8395a495eecbe98e740e258af41e552", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0027E1A8D0", "query_cov": 0.890625, "subject_cov": 1.0, "identity": 0.982, "score": 119.0, "evalue": 3.97e-34, "valid": true, "uniref50_id": "UniRef50_UPI0027E1A8D0" }, "pscc": { "uniref50_id": "UniRef50_UPI0027E1A8D0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0027E1A8D0" ] }, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 246882, "stop": 247265, "observations": { "mutation": [ 246900, 246987 ], "stop": [ 246900, 246987 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q50284", "query_cov": 1.0, "subject_cov": 0.5039370078740157, "identity": 1.0, "score": 134.0, "evalue": 1.68e-39, "gene_start": 1, "gene_end": 64, "reference_start": 64, "reference_end": 127, "reference_sequence": "MLWPFRWVWWKRVLTSQTRAPAKPNPLTVPPTCTWWSLRKLPNPTKLDDDLKNLLDPNEVRARMLKSFGTENFTQPQPQPQALKTTTPVFGTSSGNLGSVLSGGGYHAGLKHHQSTVTRSTGEWVDR" }, "paralog": false, "identity": 0.984251968503937, "score": 265.0, "evalue": 3.62e-93, "description": "Internal stop codon at 246900, 246987. Nonsense mutation around 246900, 246987.", "psc": { "uniref90_id": "UniRef90_Q50284", "product": "Putative adhesin P1-like protein MPN_203", "uniref50_id": "UniRef50_Q50284", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q50284", "UniRef:UniRef50_Q50284" ] }, "pscc": { "uniref50_id": "UniRef50_Q50284", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50284" ], "product": "Putative adhesin P1-like protein MPN_203" } }, "id": "APKECPOBEN_476", "locus": "GCA002090215_00440" }, { "type": "cds", "sequence": "contig_1", "start": 247377, "stop": 247628, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_015344891.1", "SO:0001217", "UniParc:UPI0002B84AE1", "UniRef:UniRef100_UPI0002B84AE1", "UniRef:UniRef50_A0A0H3DNZ5", "UniRef:UniRef90_A0AB38WAH1" ], "nt": "ATGAAATTGATTAAATTAGCGAGGTTTCACCGCTTTTTAAAACAAGACCAATTGCAACTTGTTTCTTTGTTTAGCCAAAACTTTTTGCAAGTTTTGTTGACACCTTTAAATAAACCGGTCAATTTATTTAGTGAAAACACAACAATGAAAACAAATAAAAAATACGCCTTTGCGGCGTTGCTAATGCAAGCTCAAGCTAAGCTCCCAAATTCATTTAAATTCTTTCTTTCAAAAAAAGGAGGTATTACTTAA", "aa": "MKLIKLARFHRFLKQDQLQLVSLFSQNFLQVLLTPLNKPVNLFSENTTMKTNKKYAFAALLMQAQAKLPNSFKFFLSKKGGIT", "aa_hexdigest": "e5444502d0ed4feaefdf48ea6846630b", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0002B84AE1", "ncbi_nrp_id": "WP_015344891.1", "uniref100_id": "UniRef100_UPI0002B84AE1", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002B84AE1", "RefSeq:WP_015344891.1", "UniRef:UniRef100_UPI0002B84AE1" ] }, "ips": { "uniref100_id": "UniRef100_UPI0002B84AE1", "uniref90_id": "UniRef90_A0AB38WAH1" }, "psc": { "uniref90_id": "UniRef90_A0AB38WAH1", "uniref50_id": "UniRef50_A0A0H3DNZ5" }, "pscc": { "uniref50_id": "UniRef50_A0A0H3DNZ5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0H3DNZ5" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9578.361899999993, "isoelectric_point": 10.675851631164551 }, "id": "APKECPOBEN_477", "locus": "GCA002090215_00441" }, { "type": "cds", "sequence": "contig_1", "start": 247796, "stop": 248074, "strand": "+", "frame": 2, "gene": null, "product": "UPF0134 protein MPN_410", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75572", "UniRef:UniRef90_P75572" ], "nt": "GTGAACACGGAATACGTAACGCGTAAAGAGTTAAACGAGTACAAAGACTCAAACGACCAACGACTTACAAAGATTGAAACCACTTTGGCCGCCCAAGGTGAGCAAATCAACAAATTAACTCAAACTGTTGAAAAACAGGGCGAACAAATTAGAGAACTTCAAGTGGAGCAAAAAGCTCAGGGTGAGCAAATTAAAGCTCAGGGAGAAACCTTAAAGCTAATCTTGCAAACGCTGCAAAAAATGAGTGATCGCTTGGACAAAATTGATCCATCTAAATAG", "aa": "MNTEYVTRKELNEYKDSNDQRLTKIETTLAAQGEQINKLTQTVEKQGEQIRELQVEQKAQGEQIKAQGETLKLILQTLQKMSDRLDKIDPSK", "aa_hexdigest": "05e342d677ef9c6d60d35fd88b1e7dc3", "start_type": "GTG", "rbs_motif": "AACAA", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 247628, "stop": 248074, "observations": { "mutation": [ 247709 ], "stop": [ 247709 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75572", "query_cov": 1.0, "subject_cov": 0.6216216216216216, "identity": 0.9890000000000001, "score": 111.0, "evalue": 1.31e-29, "gene_start": 1, "gene_end": 92, "reference_start": 57, "reference_end": 148, "reference_sequence": "MKEKTPFKNEKEFHEMVKKTKKGTFSGWYIIDKDNNSVEFSGKFNRQFKLNKPVITVNTEYVTRKELNEYKDSNDQRLTKIETTLAAQGEQINKLTQTVEKQGEQIRELQVEQKAQGEQIKAQGETLKLILQTLQKMSDRLDKIDPSK" }, "paralog": false, "identity": 0.9932432432432432, "score": 227.0, "evalue": 1.05e-77, "description": "Internal stop codon at 247709. Nonsense mutation around 247709.", "psc": { "uniref90_id": "UniRef90_P75572", "product": "UPF0134 protein MPN_410", "uniref50_id": "UniRef50_P75572", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75572", "UniRef:UniRef50_P75572" ] }, "pscc": { "uniref50_id": "UniRef50_P75572", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75572" ], "product": "UPF0134 protein MPN_204" } }, "id": "APKECPOBEN_478", "locus": "GCA002090215_00442" }, { "type": "cds", "sequence": "contig_1", "start": 248535, "stop": 248789, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF12378.14" ], "nt": "GTGCGCGATAACACTGCCAAGGGCATCACTGCCGGCAGTGGCAGTCAACAAACCACGTATGATCCTGCGCGAACCGAGGCCACCTTGACCACCACCACCTTTGCGCTGCGCCGGTATGACCTCGCCGGGCGCGCCTTATACGACCTCGACTTTTCGAAGTTAAACCCACAAACGCCAACGCGTGATGCCAACTGCCAGATCACCTTTAACCCCTTTGGCGGCTTTGGTTTGAGTGGCAGTGCACCCCAACAGTGA", "aa": "MRDNTAKGITAGSGSQQTTYDPARTEATLTTTTFALRRYDLAGRALYDLDFSKLNPQTPTRDANCQITFNPFGGFGLSGSAPQQ", "aa_hexdigest": "813deb3d05238ecb3f81e8d29c51b6db", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF12378.14", "name": "P1_N", "length": 57, "aa_cov": 0.6666666666666666, "hmm_cov": 0.8947368421052632, "evalue": 2.1422610034019357e-07, "score": 25.99983024597168, "start": 28, "stop": 83 } ], "seq_stats": { "molecular_weight": 9065.868699999999, "isoelectric_point": 7.89913387298584 }, "id": "APKECPOBEN_479", "locus": "GCA002090215_00443" }, { "type": "cds", "sequence": "contig_1", "start": 248820, "stop": 249320, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGCCCAAGACCCCACCGATCCTTATCGGTTTGCCGTTTTACTCGTGCCGCGCAGTGTGGTGTACTATGAGCAGTTGCAAAGGGGATTAGCGCTCCCTAACCAAGGGAGTTCGTCAGGCTCCGGTCAACAAAACACCACCATTGGCGCGTATGGGCTGAAGGTGAAGAACGCCGAGGCGGACACCGCAAAGAGCAATGAAAAACTCCAGGGCGATGAATCCAAGTCTTCCAATGGATCTTCAAGCACTTCCACCACCACCCAACGTGGTTCGACCAATTCCGACACCAAAGTCAAGGCTTTAAAAATAGAGGTGAAAAAGAAATCGGACTCGGAGGACAATGGTCAGCTGCAGTTAGAAAAAAATGATCTCGCCAACGCTCCCATTAAGCGGGGCGAGGAGTCGGGTCAGTCCGTCCAACTCAAGGCGGACGATTTTGGTACTGCCCCTTCCAGTTCGGGATCAGGCGGCAACTCCAACCCCGGTTCCCCCACCCCCTGA", "aa": "MAQDPTDPYRFAVLLVPRSVVYYEQLQRGLALPNQGSSSGSGQQNTTIGAYGLKVKNAEADTAKSNEKLQGDESKSSNGSSSTSTTTQRGSTNSDTKVKALKIEVKKKSDSEDNGQLQLEKNDLANAPIKRGEESGQSVQLKADDFGTAPSSSGSGGNSNPGSPTP", "aa_hexdigest": "7a57e927cdc38ee00ed47581fc7b4353", "start_type": "GTG", "rbs_motif": "AACAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17354.60639999997, "isoelectric_point": 6.317733573913575 }, "id": "APKECPOBEN_480", "locus": "GCA002090215_00444" }, { "type": "cds", "sequence": "contig_1", "start": 249282, "stop": 249734, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGGCCGACTTGGTAACATGGCCATTGCCACCAAACACCAAAAGCGCTTGCCCGAACCAGGTGAGGTTGCCAATGTTGGCGAAGTACGCTTCAAACGGGAGGGCGATGGGGGCCGACTTGTCGGAGTCAGCTTCCTTTTGAAAGCCCTGCTTGGTGTTCGAAGTATTACTTTCATCAAACCCGGTGGTTTGGAACAAAACATCGCGCGCCTTTCAGTCCCACAACCCGTGGTGGTTTCACTTGGGCATTCTTCAACTGGGCGGATAGTTCGCGGTCATCACCTTGGGATCCAAGTGATCAACGCGATCAATGGCGGTACGATTGCGCGCATAAGGCGCATCGTACAGAATCAGGATCGAGGCGGATCATTTGGGGAGGTCCTTGTGAATTTGCTCAGTCGCAAGCCACGGCCTTCAGGGGGTGGGGGAACCGGGGTTGGAGTTGCCGCCTGA", "aa": "MGRLGNMAIATKHQKRLPEPGEVANVGEVRFKREGDGGRLVGVSFLLKALLGVRSITFIKPGGLEQNIARLSVPQPVVVSLGHSSTGRIVRGHHLGIQVINAINGGTIARIRRIVQNQDRGGSFGEVLVNLLSRKPRPSGGGGTGVGVAA", "aa_hexdigest": "afc2b504d490e5f5b324bb714361e301", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15708.054699999975, "isoelectric_point": 11.834348487854005 }, "id": "APKECPOBEN_481", "locus": "GCA002090215_00445" }, { "type": "cds", "sequence": "contig_1", "start": 250428, "stop": 250652, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATCCCGATTGCCGTTTTACCTATTGCGGGTATTTTTTTGGGTGTTGGTGATGCAATTGCCGGAAATGCAGGTGATTTAACTGGTCTGCGTTATTTCGGACTTTTCATTAAAAATGGTGGTGATGTTGTTTTTGCTAACCTACCCATTTTGTTTGCTATTGCTATAGCAATAACGTTCTCACAAGACGCTGGGGTCGCCGGATTTTCGGCCTTTGTCTTTTGA", "aa": "MIPIAVLPIAGIFLGVGDAIAGNAGDLTGLRYFGLFIKNGGDVVFANLPILFAIAIAITFSQDAGVAGFSAFVF", "aa_hexdigest": "14e5d9a47c587e87911697fbcf380fdc", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7518.7656, "isoelectric_point": 4.138697242736816 }, "id": "APKECPOBEN_482", "locus": "GCA002090215_00446" }, { "type": "cds", "sequence": "contig_1", "start": 250659, "stop": 250985, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACGGGTTTATGAGCTCGTTAATATTGCCTTTTGATAAAGCAGGCAAAATTATTACTGACACTTCGACTCCAATTGCTGGTTTTAAAGTACTTTACTACAAGAGTGTCCCTGTTCATGCTATTGCTACAACCTTGGGACTGCGAACATTATCAACCTCAGTATTCGGTGGGATTATTGTAGGTGCCTTAACTTCAGTACTTTACAAGAAGTTTTATGCCATTAGGTTACCTGATGTAATTGGATTCTTTAGCGGTACTCGTTTTGTCCCTATTATTTGTTTTGTGGTAGCGATTCCTGTTGCGTTAATCTTGTTAATGATCTGA", "aa": "MNGFMSSLILPFDKAGKIITDTSTPIAGFKVLYYKSVPVHAIATTLGLRTLSTSVFGGIIVGALTSVLYKKFYAIRLPDVIGFFSGTRFVPIICFVVAIPVALILLMI", "aa_hexdigest": "ae6474d1b9b520b7a4a9996aa1460cab", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11629.966399999988, "isoelectric_point": 9.843049049377441 }, "id": "APKECPOBEN_483", "locus": "GCA002090215_00447" }, { "type": "cds", "sequence": "contig_1", "start": 251475, "stop": 251933, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGGTCTGAACGGTTCTGGTATTTTAGGTAAAAAATTGTTACTAAGTGATGGTAATGTTTACACTATTACCTTTAAAACCTTTGCCAACACTACTCCAATTGCTTGGAGTAAAGGTGCACAGGCAGTGTTACCTTTAAATGCTAGCTCTACAATCGTTAACAATCCAACCGCTTTAGCAGCAGCTACACAAAGTAATAATAACACTAACAACATTAAACTTTACCCGGTAAATTCCTTTAGGGTAGCTGTTGAAAGTTTAAATCCAGCGCAATACTCCCAGGGTAAATTCCCCTTCATGTTGTTTGGAATTCCAGCAGCTGGTGTAGCCATGATCTTGGCAGCACCTAAAGATAGAAGAAAGGAAGCAGCTTCGATTGTTGGTAGTGCAGCATTTACCAGTTTCTTAACTGGTATTACTGAACCATTTGAATTTACCTTCTTGTTCTTAGCGCCTTGA", "aa": "MGLNGSGILGKKLLLSDGNVYTITFKTFANTTPIAWSKGAQAVLPLNASSTIVNNPTALAAATQSNNNTNNIKLYPVNSFRVAVESLNPAQYSQGKFPFMLFGIPAAGVAMILAAPKDRRKEAASIVGSAAFTSFLTGITEPFEFTFLFLAP", "aa_hexdigest": "e731937ab6c91344ce87cd581481d22b", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16049.296199999986, "isoelectric_point": 9.75440502166748 }, "id": "APKECPOBEN_484", "locus": "GCA002090215_00448" }, { "type": "cds", "sequence": "contig_1", "start": 251982, "stop": 253121, "strand": "+", "frame": 3, "gene": "ptsG", "product": "PTS system glucose-specific EIICBA component", "db_xrefs": [ "COG:COG2190", "COG:G", "EC:2.7.1.199", "GO:0005886", "GO:0008982", "GO:0009401", "GO:0016301", "GO:0090563", "SO:0001217", "UniRef:UniRef50_P47315", "UniRef:UniRef90_P75569" ], "nt": "ATGAACATCTTGGGTGCTAATGTGGGTCAAACCTTCTCTGGTTCCTTTATTGACTTTATTCTCTATGGTGCTTTACCAGATGGTCGTCGTTGGTTAGCAAACTCTTACTTAGTACCTATTATTGGACTGTTCTTAGCTGCCATCTACTTCCCAACCTTCTACTTCTTAATTAAGCACTTTAACTTGGCAACTCCAGGTCGTGGTGGTAAGTTAATTACCAAGAAGGAATACTTAGCAAGCAAAGCAGCCGCTAAAGCAGAGGGTGTAAGTGGTGTAGCAGAAAACTTTACCCAAACGCAAATTGAAGCGGGCATCTTACTCCAAGCTTACGGTGGCAAGGAAAACATTGTTGAACTAGGTGCATGTATTACCAAGTTACGGGTTACTGTTAAGAACCCCGAACTAGTTAAGGAAGAACCTATTAAAGAACTTGGAGCTGCTGGGGTAATGCGTACCACCCCAACCTTCTTTGTGGCCGTTTTTGGTACCCGCGCTGCCGTTTACAAATCTGCTATGCAGGACATTATCCAGGGCAAGGTTAACTGGGAAGCGTTGCAAAAGGTTATTAATACAGATCAACTAGCTGTTGAGCCTAAAGAAACCACTCCACCAAAGGAAGTAATGCCGGTGGTACAAGACGAAATTGTGATTCTGTCCCCAGTTAACGGTACCTTAAAATCACTCAATCAAGTACCCGATGAAACCTTTAAGCAAAAGCTAGTGGGTGAAGGTGTTGCCATTGTACCGAGCGATGGTCACTTTAAAGCCCCTGGTGAAGCAGGTGTGAAAACGGAGTTAGCTTTCCCTGGTGGTCACGCTTACATCTTTGACATTGATGGCATTAAGGTAATGTTGCACATTGGGATTGACACTGTGCAAATTAACGCCAAAAAACAACCGGGCGAACCACTAGAAGTGTTTGACATTAAGACCAAACAGGGTGAGTACACTAAGGAAAAGAGTGAAAGTGTAGTGGAAGTTGATTTAAAGAAACTCAGCAAGAAGTACAACCCAATTACCCCATTTGTGGTAATGAAGGAATCACTAGAAAACTTTAAATTAGTGCCAATCCGCCAACGCGGTGAGATTAAAGTGGGCCAACCAATCTTTAAACTAGTCTACAAGAAGTCACAGGCATAA", "aa": "MNILGANVGQTFSGSFIDFILYGALPDGRRWLANSYLVPIIGLFLAAIYFPTFYFLIKHFNLATPGRGGKLITKKEYLASKAAAKAEGVSGVAENFTQTQIEAGILLQAYGGKENIVELGACITKLRVTVKNPELVKEEPIKELGAAGVMRTTPTFFVAVFGTRAAVYKSAMQDIIQGKVNWEALQKVINTDQLAVEPKETTPPKEVMPVVQDEIVILSPVNGTLKSLNQVPDETFKQKLVGEGVAIVPSDGHFKAPGEAGVKTELAFPGGHAYIFDIDGIKVMLHIGIDTVQINAKKQPGEPLEVFDIKTKQGEYTKEKSESVVEVDLKKLSKKYNPITPFVVMKESLENFKLVPIRQRGEIKVGQPIFKLVYKKSQA", "aa_hexdigest": "a5a856f4cedb2962e2a773fd71348971", "start_type": "ATG", "rbs_motif": null, "genes": [ "ptsG" ], "truncated": "5-prime", "pseudogene": { "start": 251682, "stop": 253121, "observations": { "mutation": [ 251931 ], "stop": [ 251931 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75569", "query_cov": 1.0, "subject_cov": 0.4031914893617021, "identity": 1.0, "score": 731.0, "evalue": 1.55e-257, "gene_start": 1, "gene_end": 379, "reference_start": 562, "reference_end": 940, "reference_sequence": "MQIKAQDTGQQKKSCLLSNIRNKWKNRNRGSFRQWVGKLSNGLMIPIAVLPIAGIFLGVGDAIAGNAGDLTGLRYFGLFIKNGGDVVFANLPILFAIAIAITFSQDAGVAGFSAFVFWAAMNGFMSSLILPFDKAGKIITDTSTPIAGFKVLYNKSVPVHAIATTLGLRTLSTSVFGGIIVGALTSVLYKKFYAIRLPDVIGFFSGTRFVPIICFVVAIPVALILLMIWPAVSIGLNAIGTGLGFLGGKGYGANSLIFGYIERSLIPFGVHHAFYAPLWYTSAGGSLQEIVNQQVWIRPDFHLSDNYVARVIGWVDPNNSSMYIIPGALNGQNGSSTGNTMSKDLNGALSAYMSKESTAFLTWKDLVDGLTFKGNFDKMAENGLLDGSNKIWLGLNGSGILGKKLLLSDGNVYTITFKTFANTTPIAWSKGAQAVLPLNASSTIVNNPTALAAATQSNNNTNNIKLYPVNSFRVAVESLNPAQYSQGKFPFMLFGIPAAGVAMILAAPKDRRKEAASIVGSAAFTSFLTGITEPFEFTFLFLAPWLFYGVHAVLAAVSFWLMNILGANVGQTFSGSFIDFILYGALPDGRRWLANSYLVPIIGLFLAAIYFPTFYFLIKHFNLATPGRGGKLITKKEYLASKAAAKAEGVSGVAENFTQTQIEAGILLQAYGGKENIVELGACITKLRVTVKNPELVKEEPIKELGAAGVMRTTPTFFVAVFGTRAAVYKSAMQDIIQGKVNWEALQKVINTDQLAVEPKETTPPKEVMPVVQDEIVILSPVNGTLKSLNQVPDETFKQKLVGEGVAIVPSDGHFKAPGEAGVKTELAFPGGHAYIFDIDGIKVMLHIGIDTVQINAKKQPGEPLEVFDIKTKQGEYTKEKSESVVEVDLKKLSKKYNPITPFVVMKESLENFKLVPIRQRGEIKVGQPIFKLVYKKSQA" }, "paralog": false, "identity": 0.9979123173277662, "score": 938.0, "evalue": 0.0, "description": "Internal stop codon at 251931. Nonsense mutation around 251931.", "psc": { "uniref90_id": "UniRef90_P75569", "gene": "ptsG", "product": "PTS system glucose-specific EIICBA component", "ec_ids": [ "2.7.1.199" ], "uniref50_id": "UniRef50_P47315", "cog_id": "COG2190", "cog_category": "G", "go_ids": [ "GO:0005886", "GO:0016301", "GO:0008982", "GO:0090563", "GO:0009401" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75569", "EC:2.7.1.199", "UniRef:UniRef50_P47315", "COG:COG2190", "COG:G", "GO:0005886", "GO:0016301", "GO:0008982", "GO:0090563", "GO:0009401" ] }, "pscc": { "uniref50_id": "UniRef50_P47315", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47315" ], "product": "PTS system glucose-specific EIICBA component" } }, "id": "APKECPOBEN_485", "locus": "GCA002090215_00449" }, { "type": "cds", "sequence": "contig_1", "start": 253731, "stop": 254066, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAGAACTGATTACAACACCTGTAGAAACCACCGCCAAAGCGGAACTCGTTTCCCTAGCTAAGCTCGGGGAAATGCGTACCCATGTCGGAATGGTTAAGCGTTACTGGAACCCCAAAATGGGCTTTTTTATTGAACCAGAACGCAAGCACAATAACGATCACTTTGTATTAGAGTTACAACGTCAATCTTTGCAAACAGCTTACAACTACGTCAAGGAAGTAGCACAAAACAACGGTCAAATTCTCTTCGTTGGTACTAAGAACGATTATGTGAAAAAACTCGTTAATAATATCGCTAAACGGGTTGATGTTGCTTTTATTACTCAGCGCTGA", "aa": "MSELITTPVETTAKAELVSLAKLGEMRTHVGMVKRYWNPKMGFFIEPERKHNNDHFVLELQRQSLQTAYNYVKEVAQNNGQILFVGTKNDYVKKLVNNIAKRVDVAFITQR", "aa_hexdigest": "da1e5dd8d5b14441d7e086c7a646a186", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12839.687799999985, "isoelectric_point": 9.59897174835205 }, "id": "APKECPOBEN_486", "locus": "GCA002090215_00450" }, { "type": "cds", "sequence": "contig_1", "start": 254067, "stop": 254615, "strand": "+", "frame": 3, "gene": "rpsB", "product": "30S ribosomal protein S2", "db_xrefs": [ "GO:0003735", "GO:0006412", "GO:0015935", "KEGG:K02967", "SO:0001217", "UniRef:UniRef50_UPI00132F76CA", "UniRef:UniRef90_UPI00132F76CA" ], "nt": "TTGGGTGGTACTTTAACTAACTTTAAAACACTTTCAATTTCAATTAATAAACTCAATAAGTTAGTGGAAAAACAAGCGGAAAACGCGGCTGATTTAACTAAAAAGGAAAACTTGATGCTTTCCCGCGAAATTGAACGCTTGGAGAAGTTCTTTGGCGGGGTAAAATCACTTAAACGTTTACCGAACTTACTAATTGTGGATGACCCGGTATACGAAAAGAATGCGGTAGCGGAGGCTAACATCTTGAGAATTCCTGTAGTAGCGTTGTGTAACACTAACACCAATCCGGAATTAGTTGACTTTATTATTCCCGCTAACAACCACCAACCCCAATCAACCTGCTTGTTGATGAACTTATTAGCTGATGCGGTGGCTGAAGCTAAGGCAATGCCAACCATGTTTGCTTACAAACCGGATGAGGAAATTCAAATTGAAATTCCCCAAAAACAAGAGGCACCACGCCAAGTTGTAAACCGTGCAAATTCTAAACAAATTACCTCTCAACGGTTAAACATTACTCGCAACCCAGAAGTTCTCACCCGTGAATAA", "aa": "MGGTLTNFKTLSISINKLNKLVEKQAENAADLTKKENLMLSREIERLEKFFGGVKSLKRLPNLLIVDDPVYEKNAVAEANILRIPVVALCNTNTNPELVDFIIPANNHQPQSTCLLMNLLADAVAEAKAMPTMFAYKPDEEIQIEIPQKQEAPRQVVNRANSKQITSQRLNITRNPEVLTRE", "aa_hexdigest": "4b661aa5aaf18bb7e6605bb34acf730f", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00132F76CA", "query_cov": 1.0, "subject_cov": 0.9054726368159204, "identity": 0.9890000000000001, "score": 341.0, "evalue": 3.71e-118, "valid": true, "gene": "rpsB", "product": "30S ribosomal protein S2", "uniref50_id": "UniRef50_UPI00132F76CA", "kegg_orthology_id": "K02967", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015935" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00132F76CA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00132F76CA" ], "product": "30S ribosomal protein S2" }, "genes": [ "rpsB" ], "id": "APKECPOBEN_487", "locus": "GCA002090215_00451" }, { "type": "cds", "sequence": "contig_1", "start": 254869, "stop": 255423, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTGGCCAACTCCCTGATTGGCTCCATTCAGGAATTTAAAGCGCAAAAGTCAGCTCACACCTTAAAGTCCCTAACGCAACCCTTTACGCGGGTATTCCGTGAAGAAGGCTTGGTTTCGTTACCGGTGGGTGAAGTTGTTGTCGGTGACATTATCTTTCTGGAAGCGGGTGATATTATTCCCGCTGACGGGAAGGTGCTGCAAGCGAACCACCTCCGCTGTATGGAGAGCTTTTTAACTGGTGAATCAGTCCCAGTTGATAAAAGTGTGGTTAACACCGGTGGCAAGGGTTTGTTGGAACAAACAAACCTACTCTTTTCTGGTGCCCAAGTGGTCTTTGGTAGTGGTGTATTCGAAGTAACTGCTGTGGGGTTAAATACCCAAGTCGGTCAAATTGTCAAAACAGTGGACAGTAGTGCAACTAAGCTATCGCCCCTACAGCAAAAACTGGAAAAAGTGGGGAAGTGGTTTAGCTGGTTTGGCCTTGGTTTATTCGTTGTCGTTTTCCTAGTGCAGTTAGGGTTACTTGGTTTTCACAACTTTAGCGCTAACTGA", "aa": "MLANSLIGSIQEFKAQKSAHTLKSLTQPFTRVFREEGLVSLPVGEVVVGDIIFLEAGDIIPADGKVLQANHLRCMESFLTGESVPVDKSVVNTGGKGLLEQTNLLFSGAQVVFGSGVFEVTAVGLNTQVGQIVKTVDSSATKLSPLQQKLEKVGKWFSWFGLGLFVVVFLVQLGLLGFHNFSAN", "aa_hexdigest": "cde4ae56015048cc887e0fd91da2a783", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19722.599000000002, "isoelectric_point": 6.286415290832521 }, "id": "APKECPOBEN_488", "locus": "GCA002090215_00452" }, { "type": "cds", "sequence": "contig_1", "start": 255481, "stop": 256332, "strand": "+", "frame": 1, "gene": null, "product": "cation-transporting P-type ATPase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000A405064", "UniRef:UniRef90_UPI000A405064" ], "nt": "GTGACCTTTATTAACGTTATTTTTGCCTTAAGTGTGCAGAAGCTCACCAAACAAAAGGCAATTATTAAGTACCTAGCTGCAATCGAAACACTCGGTGGCGTGCAAATCATTTGTACCGACAAAACTGGTACTTTAACACAAAACAAGATGAAGGTCGTGGACTATTTCTGTTTTAGTAACACTACCCAAACAGACTTAGCACGTGCTTTGTGTTTGTGCAACAATGCTACAGTCAACACTAACGGAGACAGCACCGGTGATCCAACTGAAATTGCCCTTTTGCAATGGTTGGACCGTGATGGTTTGGAATTAAACCACTACACACGCGTGTACGAAAAGGCATTTGATTCCAACCGCAAGCTTATGAGTGTGGTGGTGCAAAAGGACAACCGTTTTATTGTGATTGTCAAGGGCGCACACGATGTCTTGTTACCGTTATGTAAGGGCTTGGATAGCAACCAAATTAAGCCTTTAATTGATGAACGTGCTAGCAACGGCTTGCGCAACTTAGCGGTGGGACTCAAGGTTTTATACTGCTTTGATCCAGAAAATACCCAAACTGTCAACGAATTGGAAAGCGAACTCGACTTTTTGGGTTCTGTTAGCTTACAAGATCCACCACGAATTGAAAGTAAAGCTGCCATTATGGCGTGTCACCAAGCCAACATTACCCCAATTATGATCACTGGTGATCACCTCAAAACCGCTACTGCAATTGCCAAAGAATTGGGCATTCTGACCGATGAAAGGCAAGCCATTTTAGGAGTCGATTTAGACCCCGCTAAAATTATGGAGTACCGCGTCTTTGCCAGAGTAACGCCGCAGCAAAAGCTAGAAATAGTTAATGCCTGA", "aa": "MTFINVIFALSVQKLTKQKAIIKYLAAIETLGGVQIICTDKTGTLTQNKMKVVDYFCFSNTTQTDLARALCLCNNATVNTNGDSTGDPTEIALLQWLDRDGLELNHYTRVYEKAFDSNRKLMSVVVQKDNRFIVIVKGAHDVLLPLCKGLDSNQIKPLIDERASNGLRNLAVGLKVLYCFDPENTQTVNELESELDFLGSVSLQDPPRIESKAAIMACHQANITPIMITGDHLKTATAIAKELGILTDERQAILGVDLDPAKIMEYRVFARVTPQQKLEIVNA", "aa_hexdigest": "6826b544697c084abe1f0817438f3253", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 255181, "stop": 256243, "observations": { "insertion": [ 256168 ], "mutation": [ 255421 ], "stop": [ 255421, 256217 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000A405064", "query_cov": 0.8056537102473498, "subject_cov": 0.41834862385321103, "identity": 0.996, "score": 447.0, "evalue": 4.03e-153, "gene_start": 2, "gene_end": 229, "reference_start": 293, "reference_end": 520, "reference_sequence": "MNKWTGLSAAAVLESRAQHGANLIPTKKLTPFWLLFLEQFKSLVVILLLVATILSLVVAIISGVNANWLFDHNLVIEWTQPFVILITVLANSLIGSIQEFKAQKSAHTLKSLTQPFTRVFREEGLVSLPVGEVVVGDIIFLEAGDIIPADGKVLQANHLRCMESFLTGESVPVDKSVVNTGGKGLLEQTNLLFSGAQVVFGSGVFEVTAVGLNTQVGQIVKTVDSSATKLSPLQQKLEKVGKWFSWFGLGLFVVVFLVQLGLLGFHNFSANWSIALIGAIALVVAIIPEGLVTFINVIFALSVQKLTKQKAIIKYLAAIETLGGVQIICTDKTGTLTQNKMKVVDYFCFSNTTQTDLARALCLCNNATVNTNGDSTGDPTEIALLQWLDRDGLELNHYTRVYEKAFDSNRKLMSVVVQKDNRFIVIVKGAHDVLLPLCKGLDSNQIKPLIDERASNGLRNLAVGLKVLYCFDPENTQTVNELESELDFLGFVSLQDPPRIESKAAIMACHQANITPIMITXVITSKPLLQLPKNWAFWPMKGKPF" }, "paralog": false, "identity": 0.9887005649717514, "score": 677.0, "evalue": 8.5e-246, "description": "Internal stop codon at 255421, 256217. Frameshift due to insertion around 256168. Nonsense mutation around 255421.", "psc": { "uniref90_id": "UniRef90_UPI000A405064", "product": "cation-transporting P-type ATPase", "uniref50_id": "UniRef50_UPI000A405064", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000A405064", "UniRef:UniRef50_UPI000A405064" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000A405064", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000A405064" ], "product": "cation-transporting P-type ATPase" } }, "id": "APKECPOBEN_489", "locus": "GCA002090215_00453" }, { "type": "cds", "sequence": "contig_1", "start": 256417, "stop": 256812, "strand": "+", "frame": 1, "gene": null, "product": "HAD-IC family P-type ATPase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001FB434DA", "UniRef:UniRef90_UPI001FB434DA" ], "nt": "ATGGGACAAACGGGGGTTGACATTGCCAAAGATGCCGCTGATGTCATTATTAGCGATGACAACTTTGCCACAATTGTTAACGGGATTGAACAGGGTCGTAAAACCTTTTTGACCTGTAAACGGGTGTTGTTTAACCTCTTTTTAACCTCAATTGCTGGCACGATTGTCGTGTTATTGGGTTTATTTGTATTAGGGGAAGTCTTTCGTGAACAACTCAGCAAAGCAAACCATAACTTCCAGGTCTTTACCCCTACGCAACTGCTAATTATTAACCTCTTTGTTCACGGCTTTCCCGCTGTGGCCTTAGCGATCCAACCCGTCCAGGAAAAACTGATGTTAAAACCGTTTTCCACCAAAAATCTGTTTTACAACCGTGGCGGGTTCGATTTAATCTGA", "aa": "MGQTGVDIAKDAADVIISDDNFATIVNGIEQGRKTFLTCKRVLFNLFLTSIAGTIVVLLGLFVLGEVFREQLSKANHNFQVFTPTQLLIINLFVHGFPAVALAIQPVQEKLMLKPFSTKNLFYNRGGFDLI", "aa_hexdigest": "dbc40f381a642a7fb508399d7de6d4db", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 256207, "stop": 257034, "observations": { "mutation": [ 256330, 256810 ], "stop": [ 256330, 256810 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001FB434DA", "query_cov": 1.0, "subject_cov": 0.4729241877256318, "identity": 1.0, "score": 255.0, "evalue": 7.69e-84, "gene_start": 1, "gene_end": 131, "reference_start": 71, "reference_end": 201, "reference_sequence": "MGILTDERQAILGVDLDPAKIMEYRVFARVTPQQKLEIVNAWKQAGYTVAVTGDGVNDAPALVTSDVGCCMGQTGVDIAKDAADVIISDDNFATIVNGIEQGRKTFLTCKRVLFNLFLTSIAGTIVVLLGLFVLGEVFREQLSKANHNFQVFTPTQLLIINLFVHGFPAVALAIQPVQEKLMLKPFSTKNLFYNRGGFDLIWQSLLLSFLTLLFYSLGMVYAINDPELGKSGDLINRAGATCGFMVLGGSAALNSLNLMVDRPLVATNPKHYGIVXX" }, "paralog": false, "identity": 0.9890909090909091, "score": 531.0, "evalue": 4.29e-195, "description": "Internal stop codon at 256330, 256810. Nonsense mutation around 256330, 256810.", "psc": { "uniref90_id": "UniRef90_UPI001FB434DA", "product": "HAD-IC family P-type ATPase", "uniref50_id": "UniRef50_UPI001FB434DA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001FB434DA", "UniRef:UniRef50_UPI001FB434DA" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001FB434DA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001FB434DA" ], "product": "HAD-IC family P-type ATPase" } }, "id": "APKECPOBEN_490", "locus": "GCA002090215_00454" }, { "type": "cds", "sequence": "contig_1", "start": 256825, "stop": 257034, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAGCTTTTTAACCCTACTTTTCTACAGTTTAGGGATGGTCTATGCCATTAATGATCCGGAGTTAGGCAAATCCGGTGATTTAATTAACCGGGCTGGTGCGACCTGTGGTTTTATGGTGTTAGGGGGCAGTGCAGCTTTAAATTCCTTAAACCTAATGGTGGATCGACCATTGGTAGCTACTAACCCAAAGCACTATGGCATTGTGTGA", "aa": "MSFLTLLFYSLGMVYAINDPELGKSGDLINRAGATCGFMVLGGSAALNSLNLMVDRPLVATNPKHYGIV", "aa_hexdigest": "90d89e9adaf1a3081a1b760d3f328d08", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7320.5107000000025, "isoelectric_point": 6.508485221862792 }, "id": "APKECPOBEN_491", "locus": "GCA002090215_00455" }, { "type": "cds", "sequence": "contig_1", "start": 257044, "stop": 257226, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTCCTCGATCTTTGTCTTCTTATTAATTATTTTCATTAACCCCTTGGGGTTAGTGTTTAGTACATTAAAAGACCTTACTGCCCACCCGGTTTTAATTGGTTATAGCTTTGGGGGCGTGCTTTTATATATGACAATTAACGAAGTTGTTAAACTTATTAGGTTAAGTTATGGGTCTGTTTAA", "aa": "MSSIFVFLLIIFINPLGLVFSTLKDLTAHPVLIGYSFGGVLLYMTINEVVKLIRLSYGSV", "aa_hexdigest": "38c61e2afc5f1f446e6fe3015195232c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6634.966199999999, "isoelectric_point": 8.160553169250488 }, "id": "APKECPOBEN_492", "locus": "GCA002090215_00456" }, { "type": "cds", "sequence": "contig_1", "start": 257213, "stop": 259375, "strand": "+", "frame": 2, "gene": "secA", "product": "preprotein translocase subunit SecA", "db_xrefs": [ "COG:COG0653", "COG:U", "EC:7.4.2.8", "GO:0005524", "GO:0005829", "GO:0005886", "GO:0006605", "GO:0008564", "GO:0009898", "GO:0015450", "GO:0016887", "GO:0017038", "GO:0031522", "GO:0043952", "GO:0065002", "KEGG:K03070", "SO:0001217", "UniRef:UniRef50_P47318", "UniRef:UniRef90_P75559" ], "nt": "ATGGGTCTGTTTAACTTCTTGAAGTTAGTCTCCCCGCGTCATCGGATTTACCATAAGGCAAGTAAGATTGCCAACGAAGTCGAGGGACACAAAAATTACTACCGCAACCTCACTGATGTACAGCTGCTGGAAGAGTCCAATAAGCTAGTGGATTTAGTGACAAAGCAAAACTACACCATTTTGGATGTGGCGGTGGCTGCTTTGGCCTTAATTCGCGAAGTGGTCTACCGTGAAACCGGGGAGTTTGCTTACAGAGTGCAAATTATTGGTGCTTACATCGTCTTGATCGGTGACTTTGCTGAAATGATGACTGGGGAAGGGAAAACCCTGACCATTGTTTTAGCAGCCTATGTCTCGGCGTTAGAAAAACGCGGTGTCCATGTGGTTACGGTCAATGAATACCTAGCGCAGCGGGATGCTACTAACGCCACCAAGATCTTAAAGCGTGTTGGCATGACCGTTGGTTGTAACTTTGCTAACCTAGCACCACATTTAAAGCAAGCCGCGTTTGCTTGTGATGTAACTTACACCACTAACAGTGAACTCGGTTTTGATTACCTCAGGGACAATATGGTGCACCGCTTTGAGGACAAGAAGATCCGCGAGTTGCACTTTGCGATAGTAGACGAAGGCGATTCGGTTCTCATTGATGAAGCGCGCACCCCTTTAATTATTTCTGGTCCTGCCAAAAACGAGTTTGCAGCATACGTGGCTGTTGATCGCTTTGTCAAAAAGCTCAAGGAAGACGAGTACAAGATTGACCCCGAATCACGTGCCCCCGCTTTAACAGAACTTGGCATTAAGCACGCGGAAAAGAACTTTAAAACCGATAACCTGTTTGCGCTAGAAAACAGTGACCTGTTCCACAAGATTATTAACGCTTTAACCGCCGTTAAAGTTTTTGAGCAGGGCAAGGAGTACATTGTGCGTGATGGTAAGGTGTTAATTGTGGACCACTTTACTGGCCGAATCCTGGAGGGCAGGAGCTACAGTAATGGGCTCCACCAAGCGGTTCAGGCCAAGGAAATGGTCGAAATTGAACCAGAAAATGTGATAGTAGCTACGATTACCTACCAATCGTTTTTCCGTCTGTACAACCGGTTGTCAGCGGTATCGGGTACCGCTTTTACTGAATCGGAGGAATTCCTCAAGATTTACAACATGGTGGTAGTACCCGTACCTACTAACCGACCCAATATCCGCAAAGACCGTGCTGACAGCGTCTTTGGTACGCCCAACATTAAGTGGCTAGCAGTGGTCAAGGAAGTCAAACGGATCCACGAAACCGGGCGACCAATCCTAATTGGGACCGCCAACATTGATGACTCGGAACTACTTCACAACTACCTCCAAGAAGCCAACATTCCCCACGAAGTTCTCAACGCCAAGAACCACTCTCGGGAAGCGGAAATAGTCGCTAAGGCGGGCCAAAAAGGGGCGGTGACCATTTCTACCAATATGGCGGGAAGAGGTACTGACATTCGTTTAGGTGAAGGGGTCGCTGAAATGGGCGGGTTGTATGTCCTTGGTACGGAACGCAACGAATCGCGCCGGATTGACAACCAACTGCGCGGTCGGGCGGGTCGGCAAGGCGATCGCGGTGAGACCAAGTTCTTTATCTCCTTGGGTGATGCCCTGTTCAAACGCTTTGCCCATGACCGGATTGAACGCGCCATTACTAAGTTAGGGAACGATACCTTTGACAGCTCCTTCTTTTCCAAGATGTTAAGCCGTACCCAAAAACGGGTCGAAGCAATTAACTTTGACACCAGAAAGAACTTAATTGATTATGACCATGTGTTGGCCAGTCAACGGGAATTGATCTACAAACAACGCGATAAGTTCTTATTAGCCACTGATCTCAGTGACATGATCGACAAGATGTTGGAAAAGTTTGTCGAGCAGTTCTGTGACCAGTACCGCAACCCTAAGAACCAAAACCTCGTTAACCACATTGCTTTATCAGAGGCCTTAAACCTGGAATTAAACATGCACGGTGTTATTAGTCCGAAGCTGTTTGAAAACATGACTTTTGATGCGACTGTCCATAAAACCCACAGTTTAATTGGTGAAAAGATTACCAATAAGGTCAAGGTCTTAACCCCCCCAATTGCCCTCATCCGTTTTCGTGAAATTATGATCACAGCAATGGACAAGCACTGA", "aa": "MGLFNFLKLVSPRHRIYHKASKIANEVEGHKNYYRNLTDVQLLEESNKLVDLVTKQNYTILDVAVAALALIREVVYRETGEFAYRVQIIGAYIVLIGDFAEMMTGEGKTLTIVLAAYVSALEKRGVHVVTVNEYLAQRDATNATKILKRVGMTVGCNFANLAPHLKQAAFACDVTYTTNSELGFDYLRDNMVHRFEDKKIRELHFAIVDEGDSVLIDEARTPLIISGPAKNEFAAYVAVDRFVKKLKEDEYKIDPESRAPALTELGIKHAEKNFKTDNLFALENSDLFHKIINALTAVKVFEQGKEYIVRDGKVLIVDHFTGRILEGRSYSNGLHQAVQAKEMVEIEPENVIVATITYQSFFRLYNRLSAVSGTAFTESEEFLKIYNMVVVPVPTNRPNIRKDRADSVFGTPNIKWLAVVKEVKRIHETGRPILIGTANIDDSELLHNYLQEANIPHEVLNAKNHSREAEIVAKAGQKGAVTISTNMAGRGTDIRLGEGVAEMGGLYVLGTERNESRRIDNQLRGRAGRQGDRGETKFFISLGDALFKRFAHDRIERAITKLGNDTFDSSFFSKMLSRTQKRVEAINFDTRKNLIDYDHVLASQRELIYKQRDKFLLATDLSDMIDKMLEKFVEQFCDQYRNPKNQNLVNHIALSEALNLELNMHGVISPKLFENMTFDATVHKTHSLIGEKITNKVKVLTPPIALIRFREIMITAMDKH", "aa_hexdigest": "1ac3bdcbdafd767446dd7a8ddd0a26a8", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75559", "query_cov": 1.0, "subject_cov": 0.8910891089108911, "identity": 1.0, "score": 1370.0, "evalue": 0.0, "valid": true, "gene": "secA", "product": "preprotein translocase subunit SecA", "ec_ids": [ "7.4.2.8" ], "uniref50_id": "UniRef50_P47318", "cog_id": "COG0653", "cog_category": "U", "kegg_orthology_id": "K03070", "go_ids": [ "GO:0005524", "GO:0005829", "GO:0005886", "GO:0006605", "GO:0008564", "GO:0009898", "GO:0015450", "GO:0016887", "GO:0017038", "GO:0031522", "GO:0043952", "GO:0065002" ] }, "pscc": { "uniref50_id": "UniRef50_P47318", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47318" ], "product": "Protein translocase subunit SecA" }, "genes": [ "secA" ], "id": "APKECPOBEN_493", "locus": "GCA002090215_00457" }, { "type": "cds", "sequence": "contig_1", "start": 259430, "stop": 259639, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAACAAACGAGTCCCCTCAACGTTTACATTCGCGAAACCGACATCTTGTTCCAAACAATGTTGCAAAAGATTGCTCGGGACGTCATTATCCAAATTGCTAACTTAGCGACTCCCGAAGAGTTTGATGAGGAACTAATGAAGGCTAACGCCTTGAAGAAGTTGCAAGCACTTAGAGAAGCTCATGAAAAGTCCAACGAAGGACAGTAA", "aa": "MEQTSPLNVYIRETDILFQTMLQKIARDVIIQIANLATPEEFDEELMKANALKKLQALREAHEKSNEGQ", "aa_hexdigest": "9369af8055905a859603a1f7ce27b9e4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7958.0214, "isoelectric_point": 4.83963565826416 }, "id": "APKECPOBEN_494", "locus": "GCA002090215_00458" }, { "type": "cds", "sequence": "contig_1", "start": 259614, "stop": 261587, "strand": "+", "frame": 3, "gene": "uvrB", "product": "excinuclease ABC subunit UvrB", "db_xrefs": [ "COG:COG0556", "COG:L", "GO:0003677", "GO:0005524", "GO:0005737", "GO:0006289", "GO:0009380", "GO:0009381", "GO:0009432", "GO:0016887", "KEGG:K03702", "RefSeq:WP_010874568.1", "SO:0001217", "UniParc:UPI0000137EBA", "UniRef:UniRef100_P75558", "UniRef:UniRef50_P47319", "UniRef:UniRef90_P75558" ], "nt": "ATGAAAAGTCCAACGAAGGACAGTAAACTGTTCCACCTCAAGAGTAACTTTGCCCCCACTGGGGACCAACCAGCGGCGATTGCTAAGTTAGCTGAGTTTCAAACCAATGAACAGGTCTTACTCGGTGCTACGGGTACCGGTAAGACCTTTACGATTGCCAACGTTATTCAAAAGGTACAACTGCCTACAGTAGTGATTGCCCACAATAAAACGTTAGCGGGACAGCTTTATCAGGAGTTGAAGGAGTTATTCCCTAATAATGCGGTGGAGTACTTCATCTCCTACTTTGACTTTTACCAACCAGAGGCTTATCTGCCCGCTAAGGGGGTTTACATTGAAAAGAGTGCAACTGTTAACGAAGAGATTAAACGGTTACGAGTGTCCACCTTACATTCACTCTCTACCCGCAAGGATGTGATTGTGGTGGGTTCGGTAGCCAGTATTTACCCAACTTCTTCTCCCGCTGATTTCGCCCAGTACTCGCTCTGGTTAGTAGTTGGTAAGGAATACGGTTTAAGTGAGTTAAAAACGCAGTTAATTCACCTAAACTATGTTGTTAATAAACAACAGTTAACTCCGGGAAAGTTCCGCTTTCAGGGCGATGTTGTTGAAGTGTTTCCTGGTTATGCGCAGGATTATGTCTTGCGGCTTTCCTTCTTTGACCAGCAACTAGAACAAATTGCTCGCATCGATCCCTTAACTAACAAGGTATTGGAAACCCTTAACAGCTTTAAGTTAGGTCCCGCTGATGAGTACATAGTAAACCAAAATGACTTAGGAGTAGCCTTAGACACGATTAAGGCCGAACTCAAAGATCGCTTAAAGTACTTTGAACGGTTAAACTTCCCCGAACGTGCCCAGCGCTTACAAACGATTACCGAACACGATTTAGCAGATCTGAAGGCCTGGGGGGTATGTAGTGGCGTGGAAAACTATGCGCGGCATTTAGAACACCGCCCACCGCACTCCAAGCCGTATAACATCTTTGATTACTTTACTAAAGGTGAATGGCTATTGGTAGTTGATGAATCCCACCAAACGCTACCGCAAATTAAGGGGATGTACAACACCGATATTTCACGGAAACAAAGCCTAATTGAGTACGGTTTTCGCCTCCCTTCAGCTTTAGACAACCGTCCTTTATCCTATGAGGAATTCCGCCAGGGAATCAACAAGGTCATTTATGTATCCGCTACCCCTAGGGAAGAGGAAATTCAGTTAAGTCACAACAACGTGGTCGAACAGTTAGTGCGTCCCACCTACCTCTTGGACCCGGAAGTGATTGTGAAACCGAAGGACAACCAGGTTGAAGACCTAGTGAGTGAAATCATTAACCAAAGAAAACACAACGGTCGTACCTTTGTCACGGTGTTAACGATTAAGATGGCGGAAAACCTGACTGACTTTTTGAAGGAGCGCAACATTAAGGTAGCGTACATCCACAAGGATATTAAAGCCTTGGAACGTTTAATTCTGTTAACGGATTTACGCAAAGGTGAGTATGAGTGTTTAGTGGGGATTAACTTACTGCGAGAAGGTTTAGATGTTCCTGAAGTTTCACTCGTAGCCATCTTTGACGCTGACATTCCTGGATTACCACGCGATGAGCGCAGTTTAATCCAGATAATTGGCCGCGCTGCGCGAAACGTTCATGGCCGGGTGATTATGTATGCCAATACCATTAGCGAACAAATGGATAAGGCGATTAAGGAAACGCAACGCCGGCGCACCATCCAAATGGCTTACAACGAACAACACCACAAAACACCAATGACGGTGCAAAAACCAATTACCTTAAACCAGCCGATTAAGTTAAAGACCAAAAGCTCAGAGCAGCAAAAAGCTGCTTTAATTAAGCAGCTAACCAAGGAAATGAAGCAAGCAGCAGCTAACCAAAACTATGAGTTAGCGATTGAGATCCGCGACTCGATCTTTGAATTGGAAAAGCAATTTCGTGGTAAAATTAAGAGCTAG", "aa": "MKSPTKDSKLFHLKSNFAPTGDQPAAIAKLAEFQTNEQVLLGATGTGKTFTIANVIQKVQLPTVVIAHNKTLAGQLYQELKELFPNNAVEYFISYFDFYQPEAYLPAKGVYIEKSATVNEEIKRLRVSTLHSLSTRKDVIVVGSVASIYPTSSPADFAQYSLWLVVGKEYGLSELKTQLIHLNYVVNKQQLTPGKFRFQGDVVEVFPGYAQDYVLRLSFFDQQLEQIARIDPLTNKVLETLNSFKLGPADEYIVNQNDLGVALDTIKAELKDRLKYFERLNFPERAQRLQTITEHDLADLKAWGVCSGVENYARHLEHRPPHSKPYNIFDYFTKGEWLLVVDESHQTLPQIKGMYNTDISRKQSLIEYGFRLPSALDNRPLSYEEFRQGINKVIYVSATPREEEIQLSHNNVVEQLVRPTYLLDPEVIVKPKDNQVEDLVSEIINQRKHNGRTFVTVLTIKMAENLTDFLKERNIKVAYIHKDIKALERLILLTDLRKGEYECLVGINLLREGLDVPEVSLVAIFDADIPGLPRDERSLIQIIGRAARNVHGRVIMYANTISEQMDKAIKETQRRRTIQMAYNEQHHKTPMTVQKPITLNQPIKLKTKSSEQQKAALIKQLTKEMKQAAANQNYELAIEIRDSIFELEKQFRGKIKS", "aa_hexdigest": "75e1c30c7f4ab8519fd501ad689d7074", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000137EBA", "ncbi_nrp_id": "WP_010874568.1", "uniref100_id": "UniRef100_P75558", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000137EBA", "RefSeq:WP_010874568.1", "UniRef:UniRef100_P75558" ] }, "ips": { "uniref100_id": "UniRef100_P75558", "uniref90_id": "UniRef90_P75558" }, "psc": { "uniref90_id": "UniRef90_P75558", "gene": "uvrB", "product": "excinuclease ABC subunit UvrB", "uniref50_id": "UniRef50_P47319", "cog_id": "COG0556", "cog_category": "L", "kegg_orthology_id": "K03702", "go_ids": [ "GO:0003677", "GO:0005524", "GO:0005737", "GO:0006289", "GO:0009380", "GO:0009381", "GO:0009432", "GO:0016887" ] }, "pscc": { "uniref50_id": "UniRef50_P47319", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47319" ], "product": "UvrABC system protein B" }, "genes": [ "uvrB" ], "id": "APKECPOBEN_495", "locus": "GCA002090215_00459" }, { "type": "cds", "sequence": "contig_1", "start": 261590, "stop": 261997, "strand": "+", "frame": 2, "gene": null, "product": "putative protein MPNE_0244", "db_xrefs": [ "RefSeq:WP_084253831.1", "SO:0001217", "UniParc:UPI0009FFC6C7", "UniRef:UniRef100_UPI0009FFC6C7", "UniRef:UniRef50_P0CJ80", "UniRef:UniRef90_P0CJ80" ], "nt": "ATGCGTAAACTAATTAAATTAAACGTCATTGTCTTTGTCTTGTTGTACTTGGGCGAGCTGTTTGCCAGCCTTTGGTTCAAGTTAATCAGTTGCCTCAAGACACGCAACCAGTACTCCTTAAACGGGTACTATGCGTTGTTTGTCTTTGTCAACATCATCCAAAAGATGGCTAACTCTTTCCAAAAGTTAGCTTCCTCAGTTGTGTTGTTTGAAACTGAAATTAACGAATTTTTAGTTCTCTTTACTGATACAAAGAATAAACGTGAGGAGAGTGAACCAGTGCGCCAGGTGTCAACAACCCAAGAGTATCACCAGGTTACGCTCGACCAACAACACTACTTTAACCACAAACTGAGCGATTACTTCCGTTTGTTTAAGGACAAAACTTTCTTCTTTGAAATTATCTAG", "aa": "MRKLIKLNVIVFVLLYLGELFASLWFKLISCLKTRNQYSLNGYYALFVFVNIIQKMANSFQKLASSVVLFETEINEFLVLFTDTKNKREESEPVRQVSTTQEYHQVTLDQQHYFNHKLSDYFRLFKDKTFFFEII", "aa_hexdigest": "8d00bab95fab56ccf942b900014db7af", "start_type": "ATG", "rbs_motif": "AAAA", "ups": { "uniparc_id": "UPI0009FFC6C7", "ncbi_nrp_id": "WP_084253831.1", "uniref100_id": "UniRef100_UPI0009FFC6C7", "db_xrefs": [ "SO:0001217", "UniParc:UPI0009FFC6C7", "RefSeq:WP_084253831.1", "UniRef:UniRef100_UPI0009FFC6C7" ] }, "ips": { "uniref100_id": "UniRef100_UPI0009FFC6C7", "uniref90_id": "UniRef90_P0CJ80" }, "psc": { "uniref90_id": "UniRef90_P0CJ80", "product": "Uncharacterized protein MPNE_0244", "uniref50_id": "UniRef50_P0CJ80" }, "pscc": { "uniref50_id": "UniRef50_P0CJ80", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P0CJ80" ], "product": "Uncharacterized protein MG074 homolog" }, "genes": [], "id": "APKECPOBEN_496", "locus": "GCA002090215_00460" }, { "type": "cds", "sequence": "contig_1", "start": 262102, "stop": 264990, "strand": "+", "frame": 1, "gene": null, "product": "putative MG075-like protein", "db_xrefs": [ "GO:0016020", "SO:0001217", "UniRef:UniRef50_P47321", "UniRef:UniRef90_P75556" ], "nt": "ATGAAGCTTAGTGCTATTATCTCCCTATCAGTCGCTGGTACTGTGGGAACAACTGCGGTGGTAGTACCTACAACTATAACGCTTGTAAATAAGACCCACCAAGTAGAACATGAATCAGAACAATCGGATTTTCAAGATATTCGCTTTGGTCTTAATAGTGTTAAGTTGCCAAAAGCACAGCCAGCTGCGGCAACTAGAATTACCGTGGAAAACGGGACTGATAAATTAGTCAACTATAAGTCCTCACCACAACAACTCTTTTTAGCGAAGAACGCGCTTAAGGATAAACTCCAAGGTGAGTTTGATAAATTCCTAAGTGATGCGAAGGCCTTCCCAGCGCTAACCGCTGATTTACAGGAATGGGTTGACCAACAGCTGTTTAATCCAAACCAAAGTTTCTTTGATTTAAGTGCGCCCAGGTCAAACTTTACCCTTTCATCTGACAAAAAGGCTAGTTTAGACTTTATCTTCCGCTTTACTAACTTCACCGAATCCGTTCAGTTGTTAAAACTACCAGAAGGTGTATCGGTTGTAGTTGACTCCAAACAAAGCTTTGATTACTATGTCAATGCTAGTGCCCAAAAATTATTAGTTCTACCGCTGTCTTTACCAGATTACACTTTGGGTTTAAACTATATGTTTGACCACATTACTTTAAACGGTAAGGTTGTCAATAAATTTAGTTTTAATCCGTTCAAAACGAATTTAAACCTCGCCTTTAGCAACGTTTACAATGGCGTTGATGTGTTTGAAGCACAAAAGAATTTAGTAGGTAAGGGTAAATACCTAAACACCCACGTGAAGGCTGAAGACGTAAAGAAGGACGTTAATGCCAACATTAAAAACCAATTTGACATTGCCAAAATTATCGCTGAGCTAATGGGTAAAGCCCTTAAAGAATTTGGCAATCAACAAGAAGGTCAACCATTATCCTTCCTAAAGGTAATGGATAAAGTTAAAGAAGATTTTGAAAAACTGTTTAACTTAGTCCGTCCTGGATTGGGTAAATTTGTTAAGGACTTAATCCAAAGTAGTAGTCAAGCAGAAAACAAGATAACTGTCTACAAGTTAATCTTTGACAACAAAAAGACCATCTTAAACCTACTTAAAGAGCTTTCCATTCCGGAATTAAACTCTTCTTTAGGTTTAGTGGACGTCTTGTTTGATGGCATTACTGACTCTGATGGTCTCTATGAAAGGTTGCAATCTTTCAAAGACTTAATCGTTCCAGCAGTTAAAACGAATGAAAAAACCGCGGCTTTAAGTCCATTAATTGAAGAGTTATTAACCCAAAAGGATACCTATGTGTTTGACTTAATTCAAAAACACAAGGGTATCTTGACTAACTTGTTAAAGAACTTCTTAGCTGATTTCCAAAAATCAACGCCGTTTATGGCTGATCAAGTAGCCATCTTCACTGAGTTATTTGACAACGAAGGTGCGTTTGATTTATTTGGTGAGGCTGACTTTGTTGACAAGATTGCCGAACTCTTCTTAACAAAGCGTACTGTTAAAAATGGTGAAAAAATTGAAACTAAAGATTCCCTACTGGTAACATCATTAAAGAGTCTTTTAGGGGAAAAGGTAGCTGCCTTAGGTGATTTGTTAGATAGCTACATCTTTAAAAATGAATTACTTAACCGCAGTGTAGAAGTGGCTAAGGCTGAAGCTAAGGACACTAAAGGTGCTACCGATTACAAAAAGGAACAAGCTAAGGCGCTCAAAAAACTCTTTAAACACATTGGTGAAAACACTTTAAGTAAAACCAATCTCGATAAAATCACCTTAAAAGAAGTTAAAAACACCGAAAATGTTGAATTAGAAGAAACTGAAACAACCTTAAAGGTTAAAAAACTCGATGTTGAATACAAGGTAGAACTTGGCAACTTTGAAATCAAGAATGGCTTGATTAAGGCAATGCTCGAATTCTTGCCAGACACTAAAGATTTAGAAACAACTTTAGATAAACTCTTGTTCAAAGGGGAAAGCTACAAAGCGATGAAAGACAAGTACATCAAGGAAGGTTTCCCTGGTTATGGTTGGGCTAAAGGAGTAGTCCCTGGGGCCTTTGAATCTATTGAAAATACTTTTAAGAGTGCTATTGATAAAACCAAGTCCATTCGTGATCTCTTTGGCGACATGCTCTTTGGTAACGATTTAAGCAGCGTTAAAGAAACTGATTCATTCATCACCCTAGGTGGTTCGTTCGACATTAAGTATGGCGGTGAAAATCTCAATGTGTTGCCAGCTTACTACTCTTTAATTAACAGCGAAATTGGCTATCAAATTATTGGTGTAGATACCACAATTGATGCAACTAAGGTAAAAGTTGAATTAAAAAACAAAGAGTATAAGGGTAAATCGCCAGCTATTAACGGTCAGGTGAAGTTGTCACAATCATTCTTTAATGTTTGGACAAATATGTTTGACAGCATTACCAAACAAATCTTCCAAAAGAAATACGAGTTCAAAGATAACATCCAAGTGTTTGCGCGAAACGAAGATAACACATCACGTTTAGAACTTGACATTTCTGATCCTGAACAACGGGTAATTCCATTTGCTTTTGTTGATGGCTTTGGCATTCAACTCAAAGCAGTTGACAAAAACATTACGAAAGAAGCAGGTAATACTGAGCCAAAATCTCCTGTAATTCAACTTTATGAGGCACTTAATAAAGAAAAAGATCAAAAACAACAAAGTAAACAATCTCCAAAACAACTTGATACTAAAACACAGTTGGGTTACCTATTGAAATTAGGCGACAATTGGAGTAAAGATGATTACAAAAGCTTAATTGATGATACGATCATCAATAACAACTATTTAGAGGCCAGCTTTAATTCCAAGATAACGGTTGATCGCTTGGGTATTCCTATTGACCTTTGA", "aa": "MKLSAIISLSVAGTVGTTAVVVPTTITLVNKTHQVEHESEQSDFQDIRFGLNSVKLPKAQPAAATRITVENGTDKLVNYKSSPQQLFLAKNALKDKLQGEFDKFLSDAKAFPALTADLQEWVDQQLFNPNQSFFDLSAPRSNFTLSSDKKASLDFIFRFTNFTESVQLLKLPEGVSVVVDSKQSFDYYVNASAQKLLVLPLSLPDYTLGLNYMFDHITLNGKVVNKFSFNPFKTNLNLAFSNVYNGVDVFEAQKNLVGKGKYLNTHVKAEDVKKDVNANIKNQFDIAKIIAELMGKALKEFGNQQEGQPLSFLKVMDKVKEDFEKLFNLVRPGLGKFVKDLIQSSSQAENKITVYKLIFDNKKTILNLLKELSIPELNSSLGLVDVLFDGITDSDGLYERLQSFKDLIVPAVKTNEKTAALSPLIEELLTQKDTYVFDLIQKHKGILTNLLKNFLADFQKSTPFMADQVAIFTELFDNEGAFDLFGEADFVDKIAELFLTKRTVKNGEKIETKDSLLVTSLKSLLGEKVAALGDLLDSYIFKNELLNRSVEVAKAEAKDTKGATDYKKEQAKALKKLFKHIGENTLSKTNLDKITLKEVKNTENVELEETETTLKVKKLDVEYKVELGNFEIKNGLIKAMLEFLPDTKDLETTLDKLLFKGESYKAMKDKYIKEGFPGYGWAKGVVPGAFESIENTFKSAIDKTKSIRDLFGDMLFGNDLSSVKETDSFITLGGSFDIKYGGENLNVLPAYYSLINSEIGYQIIGVDTTIDATKVKVELKNKEYKGKSPAINGQVKLSQSFFNVWTNMFDSITKQIFQKKYEFKDNIQVFARNEDNTSRLELDISDPEQRVIPFAFVDGFGIQLKAVDKNITKEAGNTEPKSPVIQLYEALNKEKDQKQQSKQSPKQLDTKTQLGYLLKLGDNWSKDDYKSLIDDTIINNNYLEASFNSKITVDRLGIPIDL", "aa_hexdigest": "861423602e7e1c7e231dc5406a318299", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75556", "query_cov": 1.0, "subject_cov": 0.9339805825242719, "identity": 1.0, "score": 1835.0, "evalue": 0.0, "valid": true, "product": "Uncharacterized protein MG075 homolog", "uniref50_id": "UniRef50_P47321", "go_ids": [ "GO:0016020" ] }, "pscc": { "uniref50_id": "UniRef50_P47321", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47321" ], "product": "Uncharacterized protein MG075" }, "genes": [], "id": "APKECPOBEN_497", "locus": "GCA002090215_00461" }, { "type": "cds", "sequence": "contig_1", "start": 265030, "stop": 265194, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAGGTTCCTTACAACTTTACAGTAGTAGTGTTATCTTCCCATACGGTATTTATGACACCAGTGTTCAAGATGCTGCGAAGATTGTGAAGCGTCTGAACTTTACTGACATGGGCTTCAAACTCAACGATCCAAAACCTAACTTCTGGTTCGTTGGTTTTTAG", "aa": "MQGSLQLYSSSVIFPYGIYDTSVQDAAKIVKRLNFTDMGFKLNDPKPNFWFVGF", "aa_hexdigest": "4cdbe7fbea0ec57d6923c9bc0f5054a3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6183.027599999999, "isoelectric_point": 8.06275463104248 }, "id": "APKECPOBEN_498", "locus": "GCA002090215_00462" }, { "type": "cds", "sequence": "contig_1", "start": 265191, "stop": 265424, "strand": "-", "frame": 2, "gene": null, "product": "MPN214 family protein", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P47322", "UniRef:UniRef90_P75555" ], "nt": "GTGTGTCTCCAAGGTATAGTGCGCTTAAAAACCTGTGATGGAGTACGGTACGAAATTAGCAAGTTTAACCAATATCTAAAACTAGGTAGTATATACGCCAAGCCCAACATTAGTTTTGACGAGTATAAAGCAAAAAGTTCATCCTACCGTAAACAAACCCGCGGGTTTTGGTGGATGAACTTCTCGCTTTACCTTCTAGGTAGCTTAATCAGTATAGTAGTTAGTCTGCTCTAA", "aa": "MCLQGIVRLKTCDGVRYEISKFNQYLKLGSIYAKPNISFDEYKAKSSSYRKQTRGFWWMNFSLYLLGSLISIVVSLL", "aa_hexdigest": "25d461df7a8e49f58388b85585068976", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 265191, "stop": 265655, "observations": { "mutation": [ 265433 ], "stop": [ 265433 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75555", "query_cov": 1.0, "subject_cov": 0.5, "identity": 0.9740000000000001, "score": 152.0, "evalue": 4.64e-46, "gene_start": 1, "gene_end": 77, "reference_start": 78, "reference_end": 154, "reference_sequence": "MLVVQNILQKNDTSKYMLGTQTTNSKPREYGGLIVSTIYIVLFFAILNLTVFFNKTNNINLILKNSCVVSFVVVWLLVCLQGIVRLKTCDGARYEISKFNQYLKLGSIYAKPNISFDEYKAKSSSYRKQTRGFWWMNFSLYLLGSLISIVVSLL" }, "paralog": false, "identity": 0.9805194805194806, "score": 299.0, "evalue": 4.27e-106, "description": "Internal stop codon at 265433. Nonsense mutation around 265433.", "psc": { "uniref90_id": "UniRef90_P75555", "product": "MPN214 family protein", "uniref50_id": "UniRef50_P47322", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75555", "UniRef:UniRef50_P47322", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47322", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47322" ], "product": "Uncharacterized protein MG076" } }, "id": "APKECPOBEN_499", "locus": "GCA002090215_00463" }, { "type": "cds", "sequence": "contig_1", "start": 265431, "stop": 265649, "strand": "-", "frame": 2, "gene": null, "product": "MPN214 family protein", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P47322", "UniRef:UniRef90_P75555" ], "nt": "GTGGTTCAAAACATTCTACAAAAAAACGATACTAGTAAGTACATGTTAGGTACACAAACCACAAATTCGAAGCCGCGTGAATACGGCGGGTTAATAGTAAGCACAATTTACATTGTGCTTTTCTTCGCCATTTTGAATTTAACCGTTTTTTTCAATAAAACTAACAACATTAACCTAATTCTGAAAAACAGTTGTGTTGTTAGTTTTGTCGTTGTTTGA", "aa": "MVQNILQKNDTSKYMLGTQTTNSKPREYGGLIVSTIYIVLFFAILNLTVFFNKTNNINLILKNSCVVSFVVV", "aa_hexdigest": "35dc1714782f8651273b97459afd46a7", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 265191, "stop": 265655, "observations": { "mutation": [ 265433 ], "stop": [ 265433 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75555", "query_cov": 1.0, "subject_cov": 0.4675324675324675, "identity": 0.986, "score": 134.0, "evalue": 7.54e-39, "gene_start": 1, "gene_end": 72, "reference_start": 3, "reference_end": 74, "reference_sequence": "MLVVQNILQKNDTSKYMLGTQTTNSKPREYGGLIVSTIYIVLFFAILNLTVFFNKTNNINLILKNSCVVSFVVVWLLVCLQGIVRLKTCDGARYEISKFNQYLKLGSIYAKPNISFDEYKAKSSSYRKQTRGFWWMNFSLYLLGSLISIVVSLL" }, "paralog": false, "identity": 0.9805194805194806, "score": 299.0, "evalue": 3.54e-106, "description": "Internal stop codon at 265433. Nonsense mutation around 265433.", "psc": { "uniref90_id": "UniRef90_P75555", "product": "MPN214 family protein", "uniref50_id": "UniRef50_P47322", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75555", "UniRef:UniRef50_P47322", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47322", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47322" ], "product": "Uncharacterized protein MG076" } }, "id": "APKECPOBEN_500", "locus": "GCA002090215_00464" }, { "type": "cds", "sequence": "contig_1", "start": 265838, "stop": 266038, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTCAAGTATATTCTTAAACGTTTGGGTTTGGCAGTGTTAGCGATGTTTATCGTGATGACCATTGTCTTCTTTTTGGTTAACTCCACAGGTCAAACGCCCTTATCAGCAACCTCTTCTAAGGATTTGGAGGCTGTCAAAACACAACTAGATGCCTTTGGTTTTAACGATCCGTTAATTGTGCGATATGGTCGTTACTGA", "aa": "MFKYILKRLGLAVLAMFIVMTIVFFLVNSTGQTPLSATSSKDLEAVKTQLDAFGFNDPLIVRYGRY", "aa_hexdigest": "11d41b7e7f31b2f5c742cf530b9bb83a", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7421.7404000000015, "isoelectric_point": 9.599229621887208 }, "id": "APKECPOBEN_501", "locus": "GCA002090215_00465" }, { "type": "cds", "sequence": "contig_1", "start": 266060, "stop": 266800, "strand": "+", "frame": 2, "gene": "oppB", "product": "Oligopeptide transport system permease protein OppB", "db_xrefs": [ "COG:COG0601", "COG:EP", "GO:0005886", "GO:0015031", "GO:0015833", "GO:0055085", "SO:0001217", "UniRef:UniRef50_P75554", "UniRef:UniRef90_P75554" ], "nt": "TTGGGTACTTATTACAGCTCCCCGAACCAAACAATTGACCAAATTGTCTTTGGTCGGGTACCTAACACCCTTTACGTTGTTTTAATTTCTTTCTTTATCGGCTCTTTGTTGGGCATTATCTTCGGGATGATTTCCGGATTATTCCGTGGCAAGTTAATTGATGCGGTAATTAACGTGCTCGTTGTTTTATTTGTTTCGATTCCTTCCTTTGTGGTTGGTTTAGGTTTACTTAAGGCAGCTGGTTTGTTCCGTTTACCACCGCGCTTTATTAACTTCGATGATGCTAACTTTAACTTTGGTAACTTCTTGTTAGCCTCCATTATTCCTATTTTATCCTTGGTATTTTACACCTCAGCGGCCTTTACTTATCGGGTACGGAATGAGGTCGTGGAAGTAATGAACCAGGACTACATAAAAACAGCACGTAGTAAAGGTTTGAGTACTTTTGCTGTAGCGCTTTACCACATCTTCCGTAACTCAATTATTCCTTCTGTACCTTTATTTGTCTTTGGGATCTCTGGTGCCTTTTCGGGTGGTTTTATTATTGAATCCTTATTTGGTGTTCAGGGGGTATCACGGATCTTAATTGACTCGGTGCAAAGTAACGAAACTAACCTCGTGATGTTTAACATTATGTTCATCCAGGGGATTCCCTTACTAGCAAGTGTCTTCATTGAGTTGATTTATGTCCTAGTGGATCCTAGGATTCGGATTGCTAGTGCCGGTGGGGTGAGTCTGTGA", "aa": "MGTYYSSPNQTIDQIVFGRVPNTLYVVLISFFIGSLLGIIFGMISGLFRGKLIDAVINVLVVLFVSIPSFVVGLGLLKAAGLFRLPPRFINFDDANFNFGNFLLASIIPILSLVFYTSAAFTYRVRNEVVEVMNQDYIKTARSKGLSTFAVALYHIFRNSIIPSVPLFVFGISGAFSGGFIIESLFGVQGVSRILIDSVQSNETNLVMFNIMFIQGIPLLASVFIELIYVLVDPRIRIASAGGVSL", "aa_hexdigest": "927a60e96aff7d810b1bbbb0bc289e61", "start_type": "TTG", "rbs_motif": null, "genes": [ "oppB" ], "truncated": "both", "pseudogene": { "start": 265838, "stop": 267052, "observations": { "mutation": [ 266036, 266798, 266834, 266846 ], "stop": [ 266036, 266798, 266834, 266846 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75554", "query_cov": 1.0, "subject_cov": 0.6089108910891089, "identity": 0.996, "score": 429.0, "evalue": 8.08e-149, "gene_start": 1, "gene_end": 246, "reference_start": 75, "reference_end": 320, "reference_sequence": "MFKYIIKRLGLAVLAMFIVMTIVFFLVNSTGQTPLSATSSKDLEAVKTQLDAFGFNDPLIVRYGRYWQTLFSGSLGTYYSSPNQTIDQIVFGRVPNTLYVVLISFFIGSLLGIIFGMISGLFRGKLIDAVINVLVVLFVSIPSFVVGLGLLKAAGLFRLPPRFINFDDANFNFGNFLLASIIPILSLVFYTSAAFTYRVRNEVVEVMNQDYIKTARSKGLSTFAVALYHIFRNSIIPSVPLFVFGISGAFSGGFIIESLFGVQGVSRILIDSVQSNETNLVMFNIMFIQGIPLLASVFIELIYVLVDPRIRIASAGGVSLWTKLKFVYLRQAWLRKWRRINHTNSHNVLFNSPQHRQLLELKAIDYKHNTISLTEQQKTTLKIEPTANFVLLGTKCLKIITIHG" }, "paralog": false, "identity": 0.9851485148514851, "score": 731.0, "evalue": 2.78e-270, "description": "Internal stop codon at 266036, 266798, 266834, 266846. Nonsense mutation around 266036, 266798, 266834, 266846.", "psc": { "uniref90_id": "UniRef90_P75554", "gene": "oppB", "product": "Oligopeptide transport system permease protein OppB", "uniref50_id": "UniRef50_P75554", "cog_id": "COG0601", "cog_category": "EP", "go_ids": [ "GO:0005886", "GO:0015833", "GO:0015031", "GO:0055085" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75554", "UniRef:UniRef50_P75554", "COG:COG0601", "COG:EP", "GO:0005886", "GO:0015833", "GO:0015031", "GO:0055085" ] }, "pscc": { "uniref50_id": "UniRef50_P75554", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75554" ], "product": "Oligopeptide transport system permease protein OppB" } }, "id": "APKECPOBEN_502", "locus": "GCA002090215_00466" }, { "type": "cds", "sequence": "contig_1", "start": 266879, "stop": 267052, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTGTTCAATTCGCCCCAACACCGCCAGCTGTTAGAGCTCAAAGCAATTGATTACAAGCACAATACCATTAGCTTAACTGAACAGCAAAAAACCACCCTTAAAATTGAACCAACTGCTAACTTTGTTTTGTTAGGAACTAAATGTTTAAAGATTATTACGATCCATGGATAA", "aa": "MLFNSPQHRQLLELKAIDYKHNTISLTEQQKTTLKIEPTANFVLLGTKCLKIITIHG", "aa_hexdigest": "19b9a9f4a652cc1778d49be5d039f633", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6532.632099999999, "isoelectric_point": 9.360825538635254 }, "id": "APKECPOBEN_503", "locus": "GCA002090215_00467" }, { "type": "cds", "sequence": "contig_1", "start": 267045, "stop": 267182, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATAAGCACCAAAAATTTGACCAAAGCCTGTTTCAACGGGTAGACATTAATGTCTTAAAACGCTCTGACCAGTTGATTGGCAAACCAACCACTAACTTTGTCGAAATTATGAAGCGGTTGTTCCAAAATAAGTGA", "aa": "MDKHQKFDQSLFQRVDINVLKRSDQLIGKPTTNFVEIMKRLFQNK", "aa_hexdigest": "894d7b30ce7129b6cb20d3a36f168ca6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5436.275199999999, "isoelectric_point": 9.991906547546389 }, "id": "APKECPOBEN_504", "locus": "GCA002090215_00468" }, { "type": "cds", "sequence": "contig_1", "start": 267207, "stop": 267530, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCTAATTATTTTGTTAGCCATCATTGTACCCTTAGCATCACCTTATTCAGCCGTTACTCCAGTATCGAACAACGCTTTGGCACAGAACCTACCACCACGATACCTCTGGAACGGCGCGGGTGACATTCAAGTAGAGAAAATTACGGCACGCTCGATTGCTGAAGTGGCCCAATCAAGTGGTGTTTTGGTAGGGAAATTACCGGAGGCAAGTAGCAATCCACTCGCTACTAACGTTAAGTACAACATTGCGCCTTACCAACTAGCAGAGCTGAAAAACTACTATCCATTATTAGGCACTAACGGTTTAGGGGTTGATATTTGA", "aa": "MLIILLAIIVPLASPYSAVTPVSNNALAQNLPPRYLWNGAGDIQVEKITARSIAEVAQSSGVLVGKLPEASSNPLATNVKYNIAPYQLAELKNYYPLLGTNGLGVDI", "aa_hexdigest": "8b299a0697ca8830e9c05c81a8acf52f", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11331.995999999988, "isoelectric_point": 6.0894681930541985 }, "id": "APKECPOBEN_505", "locus": "GCA002090215_00469" }, { "type": "cds", "sequence": "contig_1", "start": 267594, "stop": 267803, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTCTTCGGCACCATTTACGGCGCGATTGCTGGGAGCTTTGTTGGACGCGCTGCTGACAACATTATGAGCCGGATCATTGAAATTATTGATTTGGTTCCTTCAATTCTCTGGATCATTGTCTTGGGAGCAACATTCCGCTTTGGTGGGGTAAAACAATTTGATGACAGTGTGGTGATCTTTACCTTAATCTTTGTCTTTTGGACCTGA", "aa": "MVFGTIYGAIAGSFVGRAADNIMSRIIEIIDLVPSILWIIVLGATFRFGGVKQFDDSVVIFTLIFVFWT", "aa_hexdigest": "77cbe5902fc33c388335cba5fa142d2c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7629.973699999999, "isoelectric_point": 4.777965354919433 }, "id": "APKECPOBEN_506", "locus": "GCA002090215_00470" }, { "type": "cds", "sequence": "contig_1", "start": 267909, "stop": 268175, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTACCAGTTGTAACTGGTCGTTTAGCAGTCGTCTTTGTGAGTTTGATTCCCGCTGTGATTGGTTATGAAGCATCACTGGTTTTCTTAGGGCTTAAACCTGCTACCGAAGTGGGACTTGGTGCTTTATTAAACCAGGTCACCAGTAGTGGTAACATAGCACTGATTACCAGCTCAATTGTGAGCTTTGCAATTCTCACCGTTTCGACCCGCGTGTTTGCTAACGCTTTAAATGACGCAATTGACCCACGGGTAATAAGGAGGTAA", "aa": "MLPVVTGRLAVVFVSLIPAVIGYEASLVFLGLKPATEVGLGALLNQVTSSGNIALITSSIVSFAILTVSTRVFANALNDAIDPRVIRR", "aa_hexdigest": "5315e020ae5c14c46bf1431cf9d7c664", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9179.767299999989, "isoelectric_point": 9.961541938781735 }, "id": "APKECPOBEN_507", "locus": "GCA002090215_00471" }, { "type": "cds", "sequence": "contig_1", "start": 268177, "stop": 268476, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00005.33" ], "nt": "ATGGCACTAAAAGCAACTAATTTTTTTACAGAACCAGAGTACAAACTGCAGGATGATTTGATCTTAGATATCCGTGATCTTCACGTGAGTTTTAAGGTTAAAGACGGCATTATGCAAGCTGTCCGTGGTGTTGATCTCAAAGTCAAAAAGGGCTCCATTGTCGGCATTGTGGGCGAATCTGGTAGTGGTAAATCGGTCTGTGTTAAGTCGATTATTGGCTTTAACGATGGCGCAAAAACGACCGCTAAGTTAATGAACTTTAAGAACATTGACATTAGCAAGATGAAAAAGCACCAGTGA", "aa": "MALKATNFFTEPEYKLQDDLILDIRDLHVSFKVKDGIMQAVRGVDLKVKKGSIVGIVGESGSGKSVCVKSIIGFNDGAKTTAKLMNFKNIDISKMKKHQ", "aa_hexdigest": "83723df501b3cd208011055721508c77", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00005.33", "name": "ABC_tran", "length": 42, "aa_cov": 0.42424242424242425, "hmm_cov": 1.0, "evalue": 1.8236701188162583e-09, "score": 33.773773193359375, "start": 42, "stop": 83 } ], "seq_stats": { "molecular_weight": 10885.681799999991, "isoelectric_point": 9.553457069396973 }, "id": "APKECPOBEN_508", "locus": "GCA002090215_00472" }, { "type": "cds", "sequence": "contig_1", "start": 268498, "stop": 269448, "strand": "+", "frame": 1, "gene": "oppD", "product": "Oligopeptide transport ATP-binding protein OppD", "db_xrefs": [ "COG:COG0444", "COG:EP", "EC:7.4.2.6", "GO:0005524", "GO:0005886", "GO:0015031", "GO:0015833", "GO:0016887", "SO:0001217", "UniRef:UniRef50_P75552", "UniRef:UniRef90_P75552" ], "nt": "GTGTCTTACATTTCCCAAGATCCACTGTTCTCGTTAAACCCCACCATGACCATCGGTCGCCAGGTTAAAGAAGCGATTTATGTGTCGTGTAAGCGCCGATATTACCAAACTAAGAGTGACCTCAAATACGATTTGCAAACAGAACGAATTGACCTGGACACTTACAAGGAAAAACTAGCACAAGCCAAGGAAACTTACAAAGCTAAAACGACTAAGGCGGCGGTCCATGCAAAAACGCTAGAAATCCTTAACTTCATTGGCATTGACCAAGCTGAAAAGCGCTTAAAGGCCTTTCCAAGTGAGTTTTCCGGGGGAATGCGGCAACGGATTGTGATCGCGATTGCCGTAGCCACCGAACCGGATTTAATTATTGCCGATGAACCGACCACGGCGTTGGATGTGACCATCCAAGCGAAGGCGTTGAACCTAATTAAACAGCTGCGTGACCTGCTCAACATTACCATTATTTTTATTAGTCACAACATCTCGTTAATTGCTAATTTCTGTGACTTTGTATACGTAATGTACGCTGGTCGATTAGTGGAAAAGGGCTTAGTTGAGGAAATCTTTACCAATCCGGTGCACCCCTACACTTGGGCATTAATGGCTTCTATCCCTGAAGGTACGGACAAAAACGCTCCGCTAACCTCAATTCCTGGGTATATTCCCAATATGTTATCCCCGCCTAAGGGCGATGCCTTTGCAGCGCGGAACCAGTTTGCACTTGCCATTGATTTCGAGCACCAACCGCCGTTCTTTGATGTAACGGAAACTCATAAGGCCGCTACCTGGCTACTCCATCCCCAAGCACCGAAGGTAGAGATGCCCGCCGATGTCAAGGAAAAGATTGCCATTACCCGCAAAGCACTCGCCATTAAAATGCCATCTCAACCTGTAAAACAACCAAAAAGCAATGGAAACAAAAAAACAAAAACAAAATCCGCTCGTTAA", "aa": "MSYISQDPLFSLNPTMTIGRQVKEAIYVSCKRRYYQTKSDLKYDLQTERIDLDTYKEKLAQAKETYKAKTTKAAVHAKTLEILNFIGIDQAEKRLKAFPSEFSGGMRQRIVIAIAVATEPDLIIADEPTTALDVTIQAKALNLIKQLRDLLNITIIFISHNISLIANFCDFVYVMYAGRLVEKGLVEEIFTNPVHPYTWALMASIPEGTDKNAPLTSIPGYIPNMLSPPKGDAFAARNQFALAIDFEHQPPFFDVTETHKAATWLLHPQAPKVEMPADVKEKIAITRKALAIKMPSQPVKQPKSNGNKKTKTKSAR", "aa_hexdigest": "aae043a35d3fe6f11b8df9911628f209", "start_type": "GTG", "rbs_motif": null, "genes": [ "oppD" ], "truncated": "5-prime", "pseudogene": { "start": 268198, "stop": 269448, "observations": { "mutation": [ 268474 ], "stop": [ 268474 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75552", "query_cov": 1.0, "subject_cov": 0.7470449172576832, "identity": 0.997, "score": 603.0, "evalue": 4.45e-216, "gene_start": 1, "gene_end": 316, "reference_start": 108, "reference_end": 423, "reference_sequence": "MALKATNFFTEPEYKLQDDLILDIRDLHVSFKVKDGIMQAVRGVDLKVKKGSIVGIVGESGSGKSVCVKSIIGFNDGAKTTAKLMNFKNIDISKMKKHQWQYYRGTYVSYISQDPLFSLNPTMTIGRQVKEAIYVSCKRRYYQTKSDLKYDLQTERIDLDTYKEKLAQAKETYKAKTTKAAVHAKTLEILNFIGIDQAEKRLKAFPSEFSGGMRQRIVIAIAVATEPDLIIADEPTTALDVTIQAKALNLIKQLRDLLNITIIFISHNISLIANFCDFVYVMYAGRLVEKGLVEEIFTNPVHPYTWALMASIPEGTDKNAPLTSIPGYIPNMLSPPKGDAFAARNQFALAIDFEHQPPFFDVTETHKAATWLLHPQAPKVEMPADVKEKIAITRKALAIKMPSQPVKQPKSNGNKKTKTKSAR" }, "paralog": false, "identity": 0.9975961538461539, "score": 820.0, "evalue": 1.17e-303, "description": "Internal stop codon at 268474. Nonsense mutation around 268474.", "psc": { "uniref90_id": "UniRef90_P75552", "gene": "oppD", "product": "Oligopeptide transport ATP-binding protein OppD", "ec_ids": [ "7.4.2.6" ], "uniref50_id": "UniRef50_P75552", "cog_id": "COG0444", "cog_category": "EP", "go_ids": [ "GO:0005886", "GO:0005524", "GO:0016887", "GO:0015833", "GO:0015031" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75552", "EC:7.4.2.6", "UniRef:UniRef50_P75552", "COG:COG0444", "COG:EP", "GO:0005886", "GO:0005524", "GO:0016887", "GO:0015833", "GO:0015031" ] }, "pscc": { "uniref50_id": "UniRef50_P75552", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75552" ], "product": "Oligopeptide transport ATP-binding protein OppD" } }, "id": "APKECPOBEN_509", "locus": "GCA002090215_00473" }, { "type": "cds", "sequence": "contig_1", "start": 269411, "stop": 270208, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00005.33" ], "nt": "ATGGAAACAAAAAAACAAAAACAAAATCCGCTCGTTAATGTTAAAGCCTTGAGCATGTTGTTCAAGGTCCGCGGTAGCTTCTTTAAAGCCTTAGATGAAATTGACTTTACCGTCAATGAAGGTGACTTCTTTGGGGTCATTGGCGAATCGGGTAGTGGTAAGTCAACCACGGGGAAGTGTTTAATCCGGTTAAATATCCCGAGTGGCGGTAAGGTGGAAATTGCTAACCACCTTATTTCTGGTAAAAAACTAACACGCGAAAACGACCACTGGTTAAAGCAAAACGTGCAGATGGTCTTTCAAGACCCATACTCTTCTTTAAACCCTACCAAGAATGTTTTAACGGTCATTTCCGAACCGTTAGTAATTACCAAAACGGTGTATGGTGAGGTAAAGGAATATCTTAAAACGCTCGCTAAACTGTCCTTTAAAACCAAGAAGGAATTGTTAAGGGAAGACTTTGAACTGGAAACCCAGTTTTACGAAAAGTTCTTTTCAAAGGTACTGTTTCACTTAGAAACTACGATTAACAAGTTTGCCCTACTCCAAGAGAGTAATAACAATAGTTCAGCCGAGTTAGCACAAACCATTTTGGGTCACACTGATGATCTAATTGAAGCATTACGGCAAGAGTTCGGTCTGGTCTATGAGTTTTCCTCATCCCAAAGCGAACCGCTACAAAAGGCCCTCAAAGACAAACAGGAAACCTTAGCACAGGACACGATTGACAAGTTAAAGCAAGAGCTGTATACAACGCAGCAAAAAGCTAAAGTTTCCACCCAGGCCTTTGCTACTTGA", "aa": "METKKQKQNPLVNVKALSMLFKVRGSFFKALDEIDFTVNEGDFFGVIGESGSGKSTTGKCLIRLNIPSGGKVEIANHLISGKKLTRENDHWLKQNVQMVFQDPYSSLNPTKNVLTVISEPLVITKTVYGEVKEYLKTLAKLSFKTKKELLREDFELETQFYEKFFSKVLFHLETTINKFALLQESNNNSSAELAQTILGHTDDLIEALRQEFGLVYEFSSSQSEPLQKALKDKQETLAQDTIDKLKQELYTTQQKAKVSTQAFAT", "aa_hexdigest": "03505af828e34312a190c749216153ea", "start_type": "ATG", "rbs_motif": "AAAA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00005.33", "name": "ABC_tran", "length": 111, "aa_cov": 0.4188679245283019, "hmm_cov": 0.8558558558558559, "evalue": 8.477415778766062e-16, "score": 54.283424377441406, "start": 31, "stop": 141 } ], "seq_stats": { "molecular_weight": 30032.951200000047, "isoelectric_point": 7.727852058410644 }, "id": "APKECPOBEN_510", "locus": "GCA002090215_00474" }, { "type": "cds", "sequence": "contig_1", "start": 270215, "stop": 271204, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAACAAACTAAGCAGAACTTAAAAGCGTACCGTGCCCAAATGGCGGAAGAGTTGCAAAACAAACCACGGATTTACCTCAATGCATGGTTGTTAACAACGAAGAACTACATTAAGGATTCACGCCAAAACACGCAGTTAACTGACGATGTCTTTGCTTTTTCTTACAACGACATGGTCGACAAAAAGCGCCGCTTAGTGTTAGTGTTATCGGAGTATTACAAGGCGTTGCCGTACTTTTATGACAATTGGATCCACCAGAACGCCGATCGTTTTGATGAATTGACCAACGCAGTCTTCTTTGACTTGATTGATGTGGTGATTGCCTTAAACCGTGATTTTGCTAATGTGGAGAGTGACGCTAAGGCCGAGTTAATCCGTTTTGTTCAGTTTATCCGTCGCTTGTGTGACTTACGCTTTGCTGCTTTGAAAAAGAGTTTTAAGAAGCAAACTAACTATAGTTTTGACTTTAACCGCGAAACCGAGTTGTTATACGCCAACAGCTGTTATGACATTAAGGAGCTCCCCCAAGTAATCCAACCATACTGGGAAAAGCTCTTTAGTGATGCTAACTATGACAAGATTGCCAAATCAGTGCAGGAACTCAACGATATTATTAGCACTGACATTGAAAAGGCATCGAATATAGCCAGTGAAATTAATACCAAGATTAGTAGTTTTAAAACTGAAATTGCGGAGTTAAAAGCTACCTTTAAAACAGAAAAAAAAGCAGAAGATCACAGTGCCCAAATTACTGGCTTAAAAACGCAAATTGCTGAAATTCAAACGCAAATCAAGCAGCAAAAACGCGAAGTCCAAAGTACCGAAAAGGCCGCTTTAAAACCAGTATTAAAGCAGTACAAGAGTGCGCTGCACCTCTATAAACGCTTTAAACAGTTGTTACGCCAATTTACCAAGCAGTTAAACCTCCTTGTCAAAAAACAGCAGGAATTAGAAAAGATTGAGGAAGGCTTGGATCTAACCATTTGA", "aa": "MQQTKQNLKAYRAQMAEELQNKPRIYLNAWLLTTKNYIKDSRQNTQLTDDVFAFSYNDMVDKKRRLVLVLSEYYKALPYFYDNWIHQNADRFDELTNAVFFDLIDVVIALNRDFANVESDAKAELIRFVQFIRRLCDLRFAALKKSFKKQTNYSFDFNRETELLYANSCYDIKELPQVIQPYWEKLFSDANYDKIAKSVQELNDIISTDIEKASNIASEINTKISSFKTEIAELKATFKTEKKAEDHSAQITGLKTQIAEIQTQIKQQKREVQSTEKAALKPVLKQYKSALHLYKRFKQLLRQFTKQLNLLVKKQQELEKIEEGLDLTI", "aa_hexdigest": "1659a76b9c7ae559a46855b7d4b29861", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 38768.90620000003, "isoelectric_point": 9.023913764953612 }, "id": "APKECPOBEN_511", "locus": "GCA002090215_00475" }, { "type": "cds", "sequence": "contig_1", "start": 271220, "stop": 271366, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTCTACCCGGTCGAAGGTGATCTCAAGAGTGAACTCAAAACACGCTTAAAGTCGTTTGGGGTGATTAACTTTGAGTACAAACGTGCTGTGCGTGAATCCCGTGTCTTTCGCTTAGTCCACTTTGGTCATGACGTGATGAAGTGA", "aa": "MFYPVEGDLKSELKTRLKSFGVINFEYKRAVRESRVFRLVHFGHDVMK", "aa_hexdigest": "c7b26262e5b846531e0f5e240b754132", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5761.681500000001, "isoelectric_point": 9.866193199157713 }, "id": "APKECPOBEN_512", "locus": "GCA002090215_00476" }, { "type": "cds", "sequence": "contig_1", "start": 271397, "stop": 271966, "strand": "+", "frame": 2, "gene": null, "product": "ABC transporter domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A4R0XTG3" ], "nt": "ATGCGTAACAAGGTGTATGAAGCATTAGACAGTGTGGGCCTCAAACGCGAGCACGCTTATCGCTATCCGCACGAGTTTTCTGGGGGTCAGCGGCAACGGATTGCGATTGCCCGCGCTTTAATTACCAAACCTAAGTTAATTATTGCAGATGAGCTTATTAGTGCTTTGGATGTATCCATTCAAGCGCAGGTGATTAACATCTTGAAGGACTTGGCTAAAAAACACAACTTAACGGTGCTCTTTATTGCCCACGACCTTTCGATGGTACAAACAGTGTGCAACCGTTTAATTATTATGCACCGCGGCAAAATTGTGGAACGGGGTAGTACGGATGAAATCTTCGCGCACCCGGTCCACCCTTACACCCGTTCCTTGATTAAGGCATCGCCAAAGTTGAGTAAGATTAACATTGACCTAGCCTCCTTTGACGAGAAGTTTACGTATGACAGTGATTATTCACTCACGAATATGCCAAGCTTCTTGAAGGTGCCAAATACCCAAGAACACGAGTTGTACTGTACTCAAGGCCAGTTTGACAGTTGGATCAAAGGAGCAAGTCGGATAAATTAA", "aa": "MRNKVYEALDSVGLKREHAYRYPHEFSGGQRQRIAIARALITKPKLIIADELISALDVSIQAQVINILKDLAKKHNLTVLFIAHDLSMVQTVCNRLIIMHRGKIVERGSTDEIFAHPVHPYTRSLIKASPKLSKINIDLASFDEKFTYDSDYSLTNMPSFLKVPNTQEHELYCTQGQFDSWIKGASRIN", "aa_hexdigest": "f94b1f67f71eb4fdf6171244cd681cf4", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0PJB1", "query_cov": 0.9417989417989417, "subject_cov": 0.9888888888888889, "identity": 0.6459999999999999, "score": 241.0, "evalue": 1.35e-78, "valid": false, "product": "Phosphate ABC transporter ATP-binding protein", "uniref50_id": "UniRef50_A0A4R0XTG3" }, "pscc": { "uniref50_id": "UniRef50_A0A4R0XTG3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A4R0XTG3" ], "product": "ABC transporter domain-containing protein" }, "genes": [], "id": "APKECPOBEN_513", "locus": "GCA002090215_00477" }, { "type": "cds", "sequence": "contig_1", "start": 272022, "stop": 272435, "strand": "+", "frame": 3, "gene": "rplK", "product": "50S ribosomal protein L11", "db_xrefs": [ "COG:COG0080", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625", "GO:0070180", "KEGG:K02867", "RefSeq:WP_010874576.1", "SO:0001217", "UniParc:UPI0000165F90", "UniRef:UniRef100_P75550", "UniRef:UniRef50_P47327", "UniRef:UniRef90_P75550" ], "nt": "ATGGCAAAAAAGACTATTACAAGAATCGCTAAGATTAACCTCTTAGGCGGGCAAGCAAAGCCCGGTCCCGCACTCGCATCTGTTGGGATTAACATGGGTGAATTCACCAAACAATTTAACGAAAAAACCAAGGACAAACAGGGCGAAATGATCCCCTGTGTGATCACCGCTTACAACGATAAGTCGTTCGACTTTATCTTAAAAACTACCCCAGTTAGCATCTTGCTTAAGCAGGCTGCAAAACTGGAAAAGGGGGCGAAAAACGCCAAAACAATTGTGGGTAAGATTACGATGGCCAAGGCCAAGGAAATTGCGCAGTACAAGTTAGTGGATCTGAACGCTAACACCGTGGAAGCAGCCCTTAAAATGGTTTTAGGTACTGCTAAACAAATGGGAATCGAGGTGATCGAATAA", "aa": "MAKKTITRIAKINLLGGQAKPGPALASVGINMGEFTKQFNEKTKDKQGEMIPCVITAYNDKSFDFILKTTPVSILLKQAAKLEKGAKNAKTIVGKITMAKAKEIAQYKLVDLNANTVEAALKMVLGTAKQMGIEVIE", "aa_hexdigest": "053c87ebf539a426ad2f8de866f82280", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000165F90", "ncbi_nrp_id": "WP_010874576.1", "uniref100_id": "UniRef100_P75550", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000165F90", "RefSeq:WP_010874576.1", "UniRef:UniRef100_P75550" ] }, "ips": { "uniref100_id": "UniRef100_P75550", "uniref90_id": "UniRef90_P75550" }, "psc": { "uniref90_id": "UniRef90_P75550", "gene": "rplK", "product": "50S ribosomal protein L11", "uniref50_id": "UniRef50_P47327", "cog_id": "COG0080", "cog_category": "J", "kegg_orthology_id": "K02867", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625", "GO:0070180" ] }, "pscc": { "uniref50_id": "UniRef50_P47327", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47327" ], "product": "Large ribosomal subunit protein uL11" }, "genes": [ "rplK" ], "id": "APKECPOBEN_514", "locus": "GCA002090215_00478" }, { "type": "cds", "sequence": "contig_1", "start": 272435, "stop": 272752, "strand": "+", "frame": 2, "gene": null, "product": "Large ribosomal subunit protein uL1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZZQ2" ], "nt": "ATGGCTAAACTATCAAAGAAAATGAAGATAGCTGTTGGTTTGGTCGATAAAACCAAGCTGTACCCGCTCCAAGAAGCAGTTGATTTAGTCAAAAAAACTTCAATTACCAAATTTAATGGCTCAGTTGATATTGCCGTTAGCCTTAATTTAGACACCACCAAGGCGGAACAACAGCTCCGCGGTGCGATTGCTTTTCCTCACAGTGTTGGCAAACCAATCCGTATTCTAGCGATCACCGATGATGAAAAGGCGGCACTGGAAGCAGGTGCTGACTTTGTTGGTGGCATTGACAAGATCAATGACATTAAAAACGGTTGA", "aa": "MAKLSKKMKIAVGLVDKTKLYPLQEAVDLVKKTSITKFNGSVDIAVSLNLDTTKAEQQLRGAIAFPHSVGKPIRILAITDDEKAALEAGADFVGGIDKINDIKNG", "aa_hexdigest": "5b19d4bfed94ee26523fc0082d49746b", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A448ZZQ2", "query_cov": 1.0, "subject_cov": 0.8467741935483871, "identity": 0.514, "score": 101.0, "evalue": 1.38e-25, "valid": false, "product": "Large ribosomal subunit protein uL1", "uniref50_id": "UniRef50_A0A448ZZQ2", "kegg_orthology_id": "K02863" }, "pscc": { "uniref50_id": "UniRef50_A0A448ZZQ2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZZQ2" ], "product": "Large ribosomal subunit protein uL1" }, "genes": [], "id": "APKECPOBEN_515", "locus": "GCA002090215_00479" }, { "type": "cds", "sequence": "contig_1", "start": 272789, "stop": 273115, "strand": "+", "frame": 2, "gene": null, "product": "Ribosomal protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2P6FDS1" ], "nt": "ATGGCCGCATTAGGTAAACTCGGGAAGCTGTTAGGTACCAAGGGTTTAATGCCTAACCCAAAAACAGAAACCGTTACTGATGATGTTCCAGCTGCTGTACGCGCTTACAAGAAGGGGAAAAAGGAATACCGCGCTGACAGCTTTGGTAACATCCACATGTCCTTAGGCCGCGTGGACAGTGCGTCCAACCACTTGGTTGAAAACGCTTTGGCACTGCTTGATCTCATTAAATCACGTAAACCAGCAACCGTTAAAGGAATTTACATTAAGAACATTGCCTTGACCACTACCATGGGTCCGAGCTTAAAGGTTAAGCTCCCTGATTAA", "aa": "MAALGKLGKLLGTKGLMPNPKTETVTDDVPAAVRAYKKGKKEYRADSFGNIHMSLGRVDSASNHLVENALALLDLIKSRKPATVKGIYIKNIALTTTMGPSLKVKLPD", "aa_hexdigest": "58ffddf1d8b4f0081ae864d6799677f9", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A2P6FDS1", "query_cov": 0.9629629629629629, "subject_cov": 0.9629629629629629, "identity": 0.5770000000000001, "score": 117.0, "evalue": 4.06e-32, "valid": false, "product": "Ribosomal protein", "uniref50_id": "UniRef50_A0A2P6FDS1", "kegg_orthology_id": "K02863" }, "pscc": { "uniref50_id": "UniRef50_A0A2P6FDS1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2P6FDS1" ], "product": "Ribosomal protein" }, "genes": [], "id": "APKECPOBEN_516", "locus": "GCA002090215_00480" }, { "type": "cds", "sequence": "contig_1", "start": 273115, "stop": 273339, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01195.25" ], "nt": "ATGGACAAGTTGCGCCTCGTTGTTGGTTTAGGTAATTTAGGTAAGCAGTACGCTGAAACCCGTCATAACGCGGGGTTTAAGGTAATTGATCGTTTACTAAGTCTTTACCATGTCCAGTTAGAGGAACGCAACAACTTAGGTGAATTCATCCTTTTAAGAAAACATAAGGTAGTGTTAGCTAAGCCAAACACTTACATGAACCACAGTGGTAAGTTTGTTAAATGA", "aa": "MDKLRLVVGLGNLGKQYAETRHNAGFKVIDRLLSLYHVQLEERNNLGEFILLRKHKVVLAKPNTYMNHSGKFVK", "aa_hexdigest": "1cbf33d032f4bf75a9e1bde68f8f53dd", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01195.25", "name": "Pept_tRNA_hydro", "length": 71, "aa_cov": 0.9324324324324325, "hmm_cov": 1.0, "evalue": 2.0561775756772046e-24, "score": 81.8519287109375, "start": 6, "stop": 74 } ], "seq_stats": { "molecular_weight": 8560.959699999996, "isoelectric_point": 10.070106697082519 }, "id": "APKECPOBEN_517", "locus": "GCA002090215_00481" }, { "type": "cds", "sequence": "contig_1", "start": 273376, "stop": 273681, "strand": "+", "frame": 1, "gene": "pth", "product": "aminoacyl-tRNA hydrolase", "db_xrefs": [ "COG:COG0193", "COG:J", "EC:3.1.1.29", "GO:0004045", "GO:0005737", "GO:0006412", "SO:0001217", "UniRef:UniRef50_P47329", "UniRef:UniRef90_P78034" ], "nt": "ATGGTGGTTTATGATGAATTAGCCTTCCCGTTAGGTACTGTTCGACTAAAAATGCAGGGTTCAGCCAACAATCATAATGGCATAAAGTCAGTAATTGCGCATTTAAATACTGAACACTTCAACCGCCTCCGGTTCGGAATTAAGTCCGATAATACTAGTAACATTCTCCATGAAGTGGTAATGTCGGAGTTTACCCCAGCCGAGCGTAATTTACTTGAAACAGCGCTTACAAAAGCGATTGAAGCACTCAAGGGTTACATTGATGGTGTGACAATGCTTAAATTAATGGAAGTTTTCAATGCCTAA", "aa": "MVVYDELAFPLGTVRLKMQGSANNHNGIKSVIAHLNTEHFNRLRFGIKSDNTSNILHEVVMSEFTPAERNLLETALTKAIEALKGYIDGVTMLKLMEVFNA", "aa_hexdigest": "15015e4edb697318eee9f8f9aaf981fa", "start_type": "ATG", "rbs_motif": null, "genes": [ "pth" ], "truncated": "5-prime", "pseudogene": { "start": 273115, "stop": 273681, "observations": { "mutation": [ 273337, 273352 ], "stop": [ 273337, 273352 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P78034", "query_cov": 1.0, "subject_cov": 0.5372340425531915, "identity": 1.0, "score": 198.0, "evalue": 4.05e-63, "gene_start": 1, "gene_end": 101, "reference_start": 88, "reference_end": 188, "reference_sequence": "MDKLRLVVGLGNLGKQYAETRHNAGFKVIDRLLSLYHVQLEERNNLGEFILLRKHKVVLAKPNTYMNHSGKFVKWACQNWNIKPDKVMVVYDELAFPLGTVRLKMQGSANNHNGIKSVIAHLNTEHFNRLRFGIKSDNTSNILHEVVMSEFTPAERNLLETALTKAIEALKGYIDGVTMLKLMEVFNA" }, "paralog": false, "identity": 0.9893617021276596, "score": 367.0, "evalue": 2.83e-132, "description": "Internal stop codon at 273337, 273352. Nonsense mutation around 273337, 273352.", "psc": { "uniref90_id": "UniRef90_P78034", "gene": "pth", "product": "aminoacyl-tRNA hydrolase", "ec_ids": [ "3.1.1.29" ], "uniref50_id": "UniRef50_P47329", "cog_id": "COG0193", "cog_category": "J", "go_ids": [ "GO:0004045", "GO:0005737", "GO:0006412" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P78034", "EC:3.1.1.29", "UniRef:UniRef50_P47329", "COG:COG0193", "COG:J", "GO:0004045", "GO:0005737", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_P47329", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47329" ], "product": "Peptidyl-tRNA hydrolase" } }, "id": "APKECPOBEN_518", "locus": "GCA002090215_00482" }, { "type": "cds", "sequence": "contig_1", "start": 273674, "stop": 273883, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCTAAAACACAATACATAGCTGGAGTATCAGGTGGTCCTGATTCAATGTTGTTGTTAAAACTGTACCACAAGAAGATTGCCTGTGTTGTACACGTTAACTACAATAAGCGGACTACTGCACTCCGCGACCAAAAAATGGTCGAAGAATACTGTAAACAGCTCAAAGTGCCCCTCATTGTGCACACGGTACCTGAAGATACCGTTTGA", "aa": "MPKTQYIAGVSGGPDSMLLLKLYHKKIACVVHVNYNKRTTALRDQKMVEEYCKQLKVPLIVHTVPEDTV", "aa_hexdigest": "59eb6744c086998d12ffa2eacba17473", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7841.224099999999, "isoelectric_point": 9.230728340148925 }, "id": "APKECPOBEN_519", "locus": "GCA002090215_00483" }, { "type": "cds", "sequence": "contig_1", "start": 274010, "stop": 274108, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAGTTGGACGCACGCAAGCGTGCCATGTATTATGGCATTAAAACACGTGGCGAATTGTATGGGATGAAGGTCTATCGTCCCTTTATTAAGTACTGA", "aa": "MQLDARKRAMYYGIKTRGELYGMKVYRPFIKY", "aa_hexdigest": "ced749f3222818e496a77cb11ec9c2b8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3947.6959000000015, "isoelectric_point": 10.087642097473143 }, "id": "APKECPOBEN_520", "locus": "GCA002090215_00484" }, { "type": "cds", "sequence": "contig_1", "start": 274265, "stop": 274543, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACCGCGTCATTGGTCAAAAGCTCTTTTCCTTAATGAAACGTGCAAAACAGTGGTTAGCTCATCCTGATGTACGTGAATTAAAGCGTTACCCACTTCCCGATCAACGTCAGTTGGTTTACAGCTTTTTAATTACCCACCACATTGATGTAACGGGGGACAAGATTGATGCCATTTTAGACTTTATCCAACCGTTTCAACAAAAGCATTACCGTCTCAAGGATGAGATCTTCTTATCCATTAAAGACGAACGGCTAACTTTACTGTACAAGAGTTAG", "aa": "MNRVIGQKLFSLMKRAKQWLAHPDVRELKRYPLPDQRQLVYSFLITHHIDVTGDKIDAILDFIQPFQQKHYRLKDEIFLSIKDERLTLLYKS", "aa_hexdigest": "e986e4fbe5ec5270d2f8cd4b97801e66", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11109.88659999999, "isoelectric_point": 9.643519401550293 }, "id": "APKECPOBEN_521", "locus": "GCA002090215_00485" }, { "type": "cds", "sequence": "contig_1", "start": 274544, "stop": 275482, "strand": "+", "frame": 2, "gene": "hprK", "product": "HPr(Ser) kinase/phosphatase", "db_xrefs": [ "COG:COG1493", "COG:T", "EC:2.7.11.-", "EC:2.7.4.-", "GO:0000155", "GO:0000160", "GO:0000287", "GO:0004674", "GO:0004712", "GO:0005524", "GO:0006109", "KEGG:K06023", "RefSeq:WP_010874580.1", "SO:0001217", "UniParc:UPI00001121FD", "UniRef:UniRef100_P75548", "UniRef:UniRef50_P75548", "UniRef:UniRef90_P75548" ], "nt": "ATGAAAAAGTTATTAGTCAAGGAGTTAATCGAACAATTTCAGGATTGTGTTAACCTCATTGATGGGCACACTAACACCAGTAATGTAATTAGAGTACCCGGATTAAAGCGGGTGGTGTTTGAAATGCTGGGGTTGTTTTCCAGCCAAATTGGCTCGGTAGCCATTTTGGGCAAACGCGAGTTTGGCTTTTTGAGTCAAAAAACGCTGGTTGAACAACAGCAAATTCTCCACAACTTACTCAAACTCAACCCACCAGCGATTATTTTGACCAAGTCCTTTACTGACCCCACGGTACTTTTACAGGTTAACCAGACTTATCAGGTACCGATCCTAAAGACCGATTTCTTTTCCACGGAACTTTCCTTTACGGTCGAAACCTACATTAACGAACAGTTTGCTACTGTTGCACAAATTCACGGCGTCTTGCTGGAAGTTTTTGGTGTAGGTGTCTTATTAACTGGTCGCAGTGGCATTGGTAAATCAGAGTGTGCCTTAGATCTAATTAACAAGAACCACCTCTTTGTCGGTGATGATGCGATTGAAATATACCGTTTGGGTAATCGCTTGTTTGGTCGCGCGCAGGAAGTAGCGAAAAAGTTTATGGAAATCCGTGGTCTTGGTATTATTAACGTCGAGCGCTTTTACGGATTGCAAATTACTAAACAGCGCACCGAAATCCAGTTAATGGTGAACTTACTGAGTTTAGAAAAACAAACGACCGTGACCTTTGAGCGTTTAGGTACCGAATTGAAAAAACAACGTTTACTCGGAGTTGATTTGAGCTTTTATGAAATTCCGATTTCCCCCGGGCGGAAGACGAGTGAAATCATTGAAAGCGCTGTAATTGATTTTAAACTTAAGCACAGTGGCTATAACTCTGCTTTAGACTTTATTGAAAACCAGAAGGCCATTTTAAAACGTAAAAAAGATGAATCCTAG", "aa": "MKKLLVKELIEQFQDCVNLIDGHTNTSNVIRVPGLKRVVFEMLGLFSSQIGSVAILGKREFGFLSQKTLVEQQQILHNLLKLNPPAIILTKSFTDPTVLLQVNQTYQVPILKTDFFSTELSFTVETYINEQFATVAQIHGVLLEVFGVGVLLTGRSGIGKSECALDLINKNHLFVGDDAIEIYRLGNRLFGRAQEVAKKFMEIRGLGIINVERFYGLQITKQRTEIQLMVNLLSLEKQTTVTFERLGTELKKQRLLGVDLSFYEIPISPGRKTSEIIESAVIDFKLKHSGYNSALDFIENQKAILKRKKDES", "aa_hexdigest": "fae0e685a2d839a331d0f12f1b2ecabd", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001121FD", "ncbi_nrp_id": "WP_010874580.1", "uniref100_id": "UniRef100_P75548", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001121FD", "RefSeq:WP_010874580.1", "UniRef:UniRef100_P75548" ] }, "ips": { "uniref100_id": "UniRef100_P75548", "uniref90_id": "UniRef90_P75548" }, "psc": { "uniref90_id": "UniRef90_P75548", "gene": "hprK", "product": "HPr(Ser) kinase/phosphatase", "ec_ids": [ "2.7.11.-", "2.7.4.-" ], "uniref50_id": "UniRef50_P75548", "cog_id": "COG1493", "cog_category": "T", "kegg_orthology_id": "K06023", "go_ids": [ "GO:0000155", "GO:0000160", "GO:0000287", "GO:0004674", "GO:0004712", "GO:0005524", "GO:0006109" ] }, "pscc": { "uniref50_id": "UniRef50_P75548", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75548" ], "product": "HPr kinase/phosphorylase" }, "genes": [ "hprK" ], "id": "APKECPOBEN_522", "locus": "GCA002090215_00486" }, { "type": "cds", "sequence": "contig_1", "start": 275589, "stop": 276401, "strand": "+", "frame": 3, "gene": "lgt", "product": "prolipoprotein diacylglyceryl transferase", "db_xrefs": [ "COG:COG0682", "COG:M", "EC:2.5.1.145", "GO:0005886", "GO:0008961", "GO:0009249", "GO:0016020", "GO:0042158", "SO:0001217", "UniRef:UniRef50_P47332", "UniRef:UniRef90_P75547" ], "nt": "ATGTTTGTCTTGAAGTTAATTTACTTTTACAAGATTCAAGATAACAGCTTTTACTTCTTTATCTTCATTGCAGTTTTAACAATGGTATTGGGCGCACGAGCTTGGTATTTTTTGATTGAAGCGGTTGATGGTCGTTCAAGTGGGAGCAACTTCTTTGACTTCCGCAACGGTGGGTTAGCAATCCAAGGTGGTGTGTTGTTAACTACGTTAGCGGGGATTATCTACTTTAACGTCTTTTTAAACATGAAGACGACCAAAACCAAAACCACCGCTAAACTATTAAACAACAAGAACCAGATTAAAACGGTTTATGTCGAGCGTAACATCTCAGTGTTTGTGATGTTGGATCTCATCGCTCCCTGTGTGTTAATTGGTCAAGCCATTGGTCGTTGGGGTAACTTCTTTAACGCGGAAGTTTATGGCGCAGCTTTAGTGGGTTCCAAAAATGATACCCTGTCAGCTGCTAACACCACCTGGGGCTTTTTAAGAATCTTAATGCCCAAAGTGTGGGACGGAATGTTTATCAATGGTAGCTTCCGCATTCCGCTTTTCTTAATCGAATCGTTCTTTAACACGATCTTCTTTGTCTTTATCTACTTTGTAATGGACCACATTAAGGGGATCCGCAGTGGGACAATTGGCTTTAGTTACTTCTTAGCGACTGGCATAGTTCGCTTAATCTTGGAAACGCAGCGTGATGAAGCGTTTAAGTACAATACCTCGATTGTGTTTAGTGCACTCTTAATTTTGGTGGGAATAGTAGGGATTATTTACTGTCAAACGTTAGCAATTAAATTGCGTGGTTATTTCTGA", "aa": "MFVLKLIYFYKIQDNSFYFFIFIAVLTMVLGARAWYFLIEAVDGRSSGSNFFDFRNGGLAIQGGVLLTTLAGIIYFNVFLNMKTTKTKTTAKLLNNKNQIKTVYVERNISVFVMLDLIAPCVLIGQAIGRWGNFFNAEVYGAALVGSKNDTLSAANTTWGFLRILMPKVWDGMFINGSFRIPLFLIESFFNTIFFVFIYFVMDHIKGIRSGTIGFSYFLATGIVRLILETQRDEAFKYNTSIVFSALLILVGIVGIIYCQTLAIKLRGYF", "aa_hexdigest": "d809685d1086983575577ed9ac438c26", "start_type": "ATG", "rbs_motif": null, "genes": [ "lgt" ], "truncated": "both", "pseudogene": { "start": 275472, "stop": 276641, "observations": { "mutation": [ 275502, 276399 ], "stop": [ 275502, 276399 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75547", "query_cov": 1.0, "subject_cov": 0.6940874035989717, "identity": 1.0, "score": 521.0, "evalue": 7.79e-185, "gene_start": 1, "gene_end": 270, "reference_start": 40, "reference_end": 309, "reference_sequence": "MNPSVSSRPPWSTAFYLGPGFPIQWYGIIVAIGIAFGILMFVLKLIYFYKIQDNSFYFFIFIAVLTMVLGARAWYFLIEAVDGRSSGSNFFDFRNGGLAIQGGVLLTTLAGIIYFNVFLNMKTTKTKTTAKLLNNKNQIKTVYVERNISVFVMLDLIAPCVLIGQAIGRWGNFFNAEVYGAALVGSKNDTLSAANTTWGFLRILMPKVWDGMFINGSFRIPLFLIESFFNTIFFVFIYFVMDHIKGIRSGTIGFSYFLATGIVRLILETQRDEAFKYNTSIVFSALLILVGIVGIIYCQTLAIKLRGYFWTYFFLYGWYKVAAFFTTLFMKDRTQACSSKFAFYEKSLPEKERSFFQLKYYNDVLPPKIYRLYDHEMLMFDKLEAVPEA" }, "paralog": false, "identity": 0.9948586118251928, "score": 775.0, "evalue": 1.75e-287, "description": "Internal stop codon at 275502, 276399. Nonsense mutation around 275502, 276399.", "psc": { "uniref90_id": "UniRef90_P75547", "gene": "lgt", "product": "prolipoprotein diacylglyceryl transferase", "ec_ids": [ "2.5.1.145" ], "uniref50_id": "UniRef50_P47332", "cog_id": "COG0682", "cog_category": "M", "go_ids": [ "GO:0005886", "GO:0008961", "GO:0009249", "GO:0016020", "GO:0042158" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75547", "EC:2.5.1.145", "UniRef:UniRef50_P47332", "COG:COG0682", "COG:M", "GO:0005886", "GO:0008961", "GO:0009249", "GO:0016020", "GO:0042158" ] }, "pscc": { "uniref50_id": "UniRef50_P47332", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47332" ], "product": "Phosphatidylglycerol--prolipoprotein diacylglyceryl transferase" } }, "id": "APKECPOBEN_523", "locus": "GCA002090215_00487" }, { "type": "cds", "sequence": "contig_1", "start": 276459, "stop": 276641, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGGATAGGACACAGGCTTGTTCTAGTAAGTTTGCATTTTACGAAAAGAGCTTACCTGAAAAGGAACGTTCCTTCTTTCAGTTGAAGTACTACAACGATGTTTTACCACCGAAAATTTACCGTTTGTACGACCATGAAATGCTCATGTTTGACAAGCTAGAAGCAGTGCCGGAAGCTTAG", "aa": "MKDRTQACSSKFAFYEKSLPEKERSFFQLKYYNDVLPPKIYRLYDHEMLMFDKLEAVPEA", "aa_hexdigest": "6ce78c1ca986456bc495a774dc51c15c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7287.329, "isoelectric_point": 6.553331184387207 }, "id": "APKECPOBEN_524", "locus": "GCA002090215_00488" }, { "type": "cds", "sequence": "contig_1", "start": 276670, "stop": 277089, "strand": "+", "frame": 1, "gene": "rpsL", "product": "30S ribosomal protein S12", "db_xrefs": [ "COG:COG0048", "COG:J", "GO:0000049", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0015935", "GO:0019843", "KEGG:K02950", "RefSeq:WP_014325410.1", "SO:0001217", "UniParc:UPI0001DD64F2", "UniRef:UniRef100_A0AB38W7X5", "UniRef:UniRef50_P92532", "UniRef:UniRef90_P47333" ], "nt": "ATGGCTACAATCGCACAATTAATTAGAAAACCGCGGAAAAAGAAGAAGGTTAAATCTAAATCACCCGCTTTACATTACAACCTCAATTTACTCAACAAAAAGGTAACCAACGTGTATTCACCACTTAAACGTGGTGTGTGTACCCGGGTTGGTACCATGACTCCAAAAAAACCGAACTCTGCTTTGCGCAAATATGCCAAGGTAAGACTTACTAACGGTTTTGAGGTGTTGGCTTATATCCCTGGTGAAGGACACAACCTCCAAGAACACAGTGTAACGCTACTCCGTGGCGGTCGGGTGAAAGACCTTCCTGGTGTGCGTTACCACATTGTTCGTGGTACCTTAGACACTGTTGGTGTGGAAAAACGCCGTCAACAACGTTCAGCTTACGGGGCTAAGAAACCAAAAGCCAAGTCCTAA", "aa": "MATIAQLIRKPRKKKKVKSKSPALHYNLNLLNKKVTNVYSPLKRGVCTRVGTMTPKKPNSALRKYAKVRLTNGFEVLAYIPGEGHNLQEHSVTLLRGGRVKDLPGVRYHIVRGTLDTVGVEKRRQQRSAYGAKKPKAKS", "aa_hexdigest": "a3ad0284a3ab459e11c3b8aec2ce0e50", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD64F2", "ncbi_nrp_id": "WP_014325410.1", "uniref100_id": "UniRef100_A0AB38W7X5", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD64F2", "RefSeq:WP_014325410.1", "UniRef:UniRef100_A0AB38W7X5" ] }, "ips": { "uniref100_id": "UniRef100_A0AB38W7X5", "uniref90_id": "UniRef90_P47333" }, "psc": { "uniref90_id": "UniRef90_P47333", "gene": "rpsL", "product": "30S ribosomal protein S12", "uniref50_id": "UniRef50_P92532", "cog_id": "COG0048", "cog_category": "J", "kegg_orthology_id": "K02950", "go_ids": [ "GO:0000049", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0015935", "GO:0019843" ] }, "genes": [ "rpsL" ], "id": "APKECPOBEN_525", "locus": "GCA002090215_00489" }, { "type": "cds", "sequence": "contig_1", "start": 277143, "stop": 277448, "strand": "+", "frame": 3, "gene": "rpsG", "product": "30S ribosomal protein S7", "db_xrefs": [ "COG:COG0049", "COG:J", "GO:0000049", "GO:0003735", "GO:0006412", "GO:0015935", "GO:0019843", "KEGG:K02992", "SO:0001217", "UniRef:UniRef50_P21469", "UniRef:UniRef90_P75545" ], "nt": "ATGCGAAAAAATCGTGCTCCCAAACGGACTGTTTTACCCGATCCCGTTTTTAACAATACGCTGGTAACCCGGATTATTAACGTCATCATGGAAGATGGCAAGAAGGGTTTAGCACAACGAATCTTATACGGTGCTTTTGATCTCATTGAACAACGCACTAAAGAAAAACCCTTAACCGTGTTTGAACGCGCTGTGGGCAACGTGATGCCGCGCTTGGAACTCCGGGTACGGCGGATTGCGGGATCCAACTATCAGGTACCTACCGAAGTACCTCAAGACCGCAAGATTGCTCTAGCACTCCGTTGA", "aa": "MRKNRAPKRTVLPDPVFNNTLVTRIINVIMEDGKKGLAQRILYGAFDLIEQRTKEKPLTVFERAVGNVMPRLELRVRRIAGSNYQVPTEVPQDRKIALALR", "aa_hexdigest": "f43a441e5f19cb8aeee3b13ea80bec62", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A519VYV2", "query_cov": 1.0, "subject_cov": 0.8278688524590164, "identity": 0.505, "score": 112.0, "evalue": 3.11e-30, "valid": false, "gene": "rpsG", "product": "30S ribosomal protein S7", "uniref50_id": "UniRef50_A0A4Q7IH49", "kegg_orthology_id": "K02992", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015935" ] }, "genes": [ "rpsG" ], "truncated": "3-prime", "pseudogene": { "start": 277143, "stop": 277610, "observations": { "mutation": [ 277446 ], "stop": [ 277446 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75545", "query_cov": 1.0, "subject_cov": 0.6516129032258065, "identity": 1.0, "score": 199.0, "evalue": 6.91e-64, "gene_start": 1, "gene_end": 101, "reference_start": 1, "reference_end": 101, "reference_sequence": "MRKNRAPKRTVLPDPVFNNTLVTRIINVIMEDGKKGLAQRILYGAFDLIEQRTKEKPLTVFERAVGNVMPRLELRVRRIAGSNYQVPTEVPQDRKIALALRWIAMFARKRHEKTMLEKIANEIIDASNNTGAAIKKKDDTHKMAEANKAFAHMRW" }, "paralog": false, "identity": 0.9935483870967742, "score": 303.0, "evalue": 3.22e-107, "description": "Internal stop codon at 277446. Nonsense mutation around 277446.", "psc": { "uniref90_id": "UniRef90_P75545", "gene": "rpsG", "product": "30S ribosomal protein S7", "uniref50_id": "UniRef50_P21469", "cog_id": "COG0049", "cog_category": "J", "kegg_orthology_id": "K02992", "go_ids": [ "GO:0000049", "GO:0003735", "GO:0006412", "GO:0015935", "GO:0019843" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75545", "UniRef:UniRef50_P21469", "COG:COG0049", "COG:J", "KEGG:K02992", "GO:0000049", "GO:0003735", "GO:0006412", "GO:0015935", "GO:0019843" ] }, "pscc": { "uniref50_id": "UniRef50_P21469", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P21469" ], "product": "Small ribosomal subunit protein uS7" } }, "id": "APKECPOBEN_526", "locus": "GCA002090215_00490" }, { "type": "cds", "sequence": "contig_1", "start": 277455, "stop": 277610, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00177.27" ], "nt": "ATGTTTGCGCGTAAGCGTCATGAAAAAACGATGTTAGAGAAGATTGCTAACGAAATTATTGATGCATCGAATAATACTGGTGCAGCCATTAAAAAGAAGGACGATACGCATAAGATGGCTGAAGCCAACAAGGCCTTTGCCCACATGCGTTGGTAA", "aa": "MFARKRHEKTMLEKIANEIIDASNNTGAAIKKKDDTHKMAEANKAFAHMRW", "aa_hexdigest": "7ac9d132e00cba7d2f89c12b1637683f", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A380FBG9", "query_cov": 0.8627450980392157, "subject_cov": 0.88, "identity": 0.75, "score": 72.8, "evalue": 4.05e-16, "valid": false, "product": "30S ribosomalprotein S7p (S5e)", "uniref50_id": "UniRef50_A0A645CV26" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00177.27", "name": "Ribosomal_S7", "length": 43, "aa_cov": 0.8431372549019608, "hmm_cov": 1.0, "evalue": 3.350582781260423e-18, "score": 61.14462661743164, "start": 2, "stop": 44 } ], "seq_stats": { "molecular_weight": 5899.768200000001, "isoelectric_point": 9.774519157409667 }, "id": "APKECPOBEN_527", "locus": "GCA002090215_00491" }, { "type": "cds", "sequence": "contig_1", "start": 277633, "stop": 277992, "strand": "+", "frame": 1, "gene": null, "product": "Elongation factor", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2S3U2I5" ], "nt": "ATGGCGCGTACAGTAGATTTAATTAACTTCCGTAATTTCGGAATTATGGCCCACATTGATGCGGGTAAAACCACCACCTCAGAGCGAATTTTATTTCACTCAGGCCGAATCCACAAGATTGGGGAAACCCATGACGGGGAGTCCGTGATGGACTGGATGGAGCAGGAAAAGGAACGCGGTATTACGATTACCTCCGCAGCTACTTCAGTTAGTTGGAAGAACTGCAGCTTAAACCTAATTGACACCCCCGGTCACGTTGACTTTACCGTAGAAGTAGAACGCAGTCTCCGTGTCCTTGATGGTGCCATTGCTGTGTTAGATGCTCAAATGGGGGTAGAACCACAAACCGAAACGGTGTGA", "aa": "MARTVDLINFRNFGIMAHIDAGKTTTSERILFHSGRIHKIGETHDGESVMDWMEQEKERGITITSAATSVSWKNCSLNLIDTPGHVDFTVEVERSLRVLDGAIAVLDAQMGVEPQTETV", "aa_hexdigest": "66d3b63bb2431530da923cf91c9296ef", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A2S3U2I5", "query_cov": 0.9663865546218487, "subject_cov": 0.8984375, "identity": 0.7909999999999999, "score": 184.0, "evalue": 2.62e-58, "valid": false, "product": "Elongation factor", "uniref50_id": "UniRef50_A0A2S3U2I5" }, "pscc": { "uniref50_id": "UniRef50_A0A2S3U2I5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2S3U2I5" ], "product": "Elongation factor" }, "genes": [], "id": "APKECPOBEN_528", "locus": "GCA002090215_00492" }, { "type": "cds", "sequence": "contig_1", "start": 278014, "stop": 279699, "strand": "+", "frame": 1, "gene": null, "product": "Elongation factor G", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q5SHN5" ], "nt": "GTGCCCCGGATTATCTTTGTCAATAAGATGGACAAAACGGGGGCTAACTTCGAGCGTTCTGTACAATCGATTCAACAGCGCTTAGGAGTAAAGGCGGTACCTATCCAATTACCAATTGGTGCGGAAAACGACTTTAACGGCATTATTGACCTGATTGAAGAGAAAGTTTACTTCTTTGATGGTGGTAAGGAAGAAAAGGCCGAGGAAAAACCAATTCCCGACCAGTTTAAGGATCAGGTCAAACAAATGCGTGCGCACCTAGTGGAAGAGGTGGCTAACTTTGACGATCAGTTAATGGCTGATTACCTTGAAGGTAAGGAAATTAGTATTGCCGACATCAAGCGCTGTATCCGCAAAGGGGTAATAGGTTGTCAGTTCTTCCCGGTGCTTTGTGGTTCTGCCTTTAAAAACAAGGGGATCAAACTGTTATTGGATGCTGTAGTAGATTTCTTACCTTCCCCAGTTGACGTACCGCAAGCCAAGGCTTATGGTGAAGATGGTAATGAGGTGTTAATCTCGGCTAGTGATGATGCACCGTTTGTTGGTTTAGCCTTTAAGGTAGCGACTGACCCATTTGTGGGACGTTTAACCTTTGTCCGCGTTTACTCCGGAGTACTTAAGTCCGGTTCTTACGTTAAGAATGTAAGAAAGAACAAAAAGGAACGGGTATCACGTTTGGTAAAAATGCACGCGCAGAACCGCAATGAAATCGAGGAAATTCGTGCTGGTGATATCTGTGCTATTATTGGATTAAAGGACACCACTACTGGAGAAACCCTAGTTGATGACAAGATTGATGTCCAACTAGAAGCGATGCAGTTCGCACAACCCGTGATCTCTTTAGCGGTGGAACCAAAAACCAAGGCAGATCAGGAAAAAATGTCGATTGCACTCTCGAAGTTAGCGGAAGAAGATCCGACCTTTAAGACCTTCACTGATCCCGAAACTGGGCAAACGATTATTGCGGGAATGGGTGAATTGCACCTAGACATCCTAGTGGATCGGATGCGCCGCGAGTTTAAGGTTGAAGTTAATGTGGGCGCACCACAAGTAAGCTTCCGTGAAACCTTCAATAAAGAAAGTGAAGTTGAGGGTAAATACATTAAACAATCGGGTGGCCGCGGTCAGTATGGTCATGTCAAGATTCGCTTTGAACCAAACAAGGACAAGGGCTTTGAGTTTGTCGACAAGATTGTTGGTGGTCGGATTCCGCGTGAGTACATTAAACCAGTGCAAGCGGGTTTGGAAAACGCTATGGCATCAGGTCCGCTAGCTGGTTATCCGATGATTGACATCAAAGCTACCTTGTTTGATGGTTCGTTCCACGAAGTTGATTCGAGTGAAATGGCCTTCAAGATTGCGGCATCCTTAGCTTTAAAAGAGGCGGGCAAGGTATGTAGTCCGGTACTGTTAGAGCCAATTATGGCAATTGAAGTGACGGTGCCAGAACAATACTTTGGGGACACCATGGGTGACATATCATCGAGAAGAGGTCTCATTGAGGGGACTGAACAACGTGATAACGTGCAGGTAATTAAGGCCAAAGTACCACTCAAAGAGATGTTTGGTTACGCCACTGATTTACGTTCCTTCTCTCAAGGTCGGGGTAACTATGTGATGCAGTTTAGCCATTACGCTGAAACCCCTAAGAGTGTGGTGAATGAGATAATTGCCACCAAGAAATAG", "aa": "MPRIIFVNKMDKTGANFERSVQSIQQRLGVKAVPIQLPIGAENDFNGIIDLIEEKVYFFDGGKEEKAEEKPIPDQFKDQVKQMRAHLVEEVANFDDQLMADYLEGKEISIADIKRCIRKGVIGCQFFPVLCGSAFKNKGIKLLLDAVVDFLPSPVDVPQAKAYGEDGNEVLISASDDAPFVGLAFKVATDPFVGRLTFVRVYSGVLKSGSYVKNVRKNKKERVSRLVKMHAQNRNEIEEIRAGDICAIIGLKDTTTGETLVDDKIDVQLEAMQFAQPVISLAVEPKTKADQEKMSIALSKLAEEDPTFKTFTDPETGQTIIAGMGELHLDILVDRMRREFKVEVNVGAPQVSFRETFNKESEVEGKYIKQSGGRGQYGHVKIRFEPNKDKGFEFVDKIVGGRIPREYIKPVQAGLENAMASGPLAGYPMIDIKATLFDGSFHEVDSSEMAFKIAASLALKEAGKVCSPVLLEPIMAIEVTVPEQYFGDTMGDISSRRGLIEGTEQRDNVQVIKAKVPLKEMFGYATDLRSFSQGRGNYVMQFSHYAETPKSVVNEIIATKK", "aa_hexdigest": "f72eaf62423e569b69770962b63c4b0f", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75544", "query_cov": 1.0, "subject_cov": 0.815406976744186, "identity": 0.8859999999999999, "score": 978.0, "evalue": 0.0, "valid": false, "gene": "fusA", "product": "elongation factor G", "uniref50_id": "UniRef50_Q5SHN5", "cog_id": "COG0480", "cog_category": "J", "kegg_orthology_id": "K02355", "go_ids": [ "GO:0003746", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0006414", "GO:0032790" ] }, "pscc": { "uniref50_id": "UniRef50_Q5SHN5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q5SHN5" ], "product": "Elongation factor G" }, "genes": [], "id": "APKECPOBEN_529", "locus": "GCA002090215_00493" }, { "type": "cds", "sequence": "contig_1", "start": 279756, "stop": 280403, "strand": "+", "frame": 3, "gene": "rpsF", "product": "30S ribosomal protein S6", "db_xrefs": [ "COG:COG0360", "COG:J", "GO:0000314", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022627", "GO:1990904", "KEGG:K02990", "RefSeq:WP_010874585.1", "SO:0001217", "UniParc:UPI00001350BA", "UniRef:UniRef100_P75543", "UniRef:UniRef50_P47336", "UniRef:UniRef90_P75543" ], "nt": "ATGCAATACAACATTATTCTTTTAGTTGATGGTTCGCTCTCGTTAGAGCAAGCTAACCAAGTGAATGAAAAACAACAGCAAACGTTAACCAACGTTGAGGGTTTGCAAACAGAATACCTTGGTTTAAAGGAGTTGGCATACCCAATTAAGAAACAACTCTCAGCGCATTATTACCGCTGGAAGTTTAGTGGTGATAACCAATCCACGAAGGACTTTAAGCGTACCGCTAACATTAACAAGCAAGTATTACGTGAACTGATTATTAACTTAGAACGTGAGTACGGCTACTTAGCTTCGATAAATCCAAAAAAGCAACAGCTGGCACTGCAAAAACGCGCTAAGTATGACGAAATCATTGCGCGGGAAAACAACCCCGAAAACCCGGATGTACCAGTAACATCAGGTTTAGCTTCCACCCAACCACGCTTATCACGTACTGAAAAGGCACAAAAACCAAAGGAAGAGCTGTGGGATGTGGTGCAAAAGATGGGAAACTTTGACAGCGTTCAAGCCAACCCATACCGCCCACGTTTCAAACGCTTTAACGCGGAACACGTTAACCAACGGCAAAACCAACAAAACAATAACAATAACCGTTTTGACCGCAACCGCAACCGTCAGCACAACCGCTTTAAGGATAAGCAATAA", "aa": "MQYNIILLVDGSLSLEQANQVNEKQQQTLTNVEGLQTEYLGLKELAYPIKKQLSAHYYRWKFSGDNQSTKDFKRTANINKQVLRELIINLEREYGYLASINPKKQQLALQKRAKYDEIIARENNPENPDVPVTSGLASTQPRLSRTEKAQKPKEELWDVVQKMGNFDSVQANPYRPRFKRFNAEHVNQRQNQQNNNNNRFDRNRNRQHNRFKDKQ", "aa_hexdigest": "873e68ea666bf750c16ec1ee7254aa62", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001350BA", "ncbi_nrp_id": "WP_010874585.1", "uniref100_id": "UniRef100_P75543", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001350BA", "RefSeq:WP_010874585.1", "UniRef:UniRef100_P75543" ] }, "ips": { "uniref100_id": "UniRef100_P75543", "uniref90_id": "UniRef90_P75543" }, "psc": { "uniref90_id": "UniRef90_P75543", "gene": "rpsF", "product": "30S ribosomal protein S6", "uniref50_id": "UniRef50_P47336", "cog_id": "COG0360", "cog_category": "J", "kegg_orthology_id": "K02990", "go_ids": [ "GO:0000314", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022627", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_P47336", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47336" ], "product": "Small ribosomal subunit protein bS6" }, "genes": [ "rpsF" ], "id": "APKECPOBEN_530", "locus": "GCA002090215_00494" }, { "type": "cds", "sequence": "contig_1", "start": 280403, "stop": 280903, "strand": "+", "frame": 2, "gene": "ssb", "product": "Single-stranded DNA-binding protein", "db_xrefs": [ "COG:COG0629", "COG:L", "GO:0003697", "GO:0006260", "GO:0009295", "RefSeq:WP_010874586.1", "SO:0001217", "UniParc:UPI0000135FAD", "UniRef:UniRef100_P75542", "UniRef:UniRef50_P75542", "UniRef:UniRef90_P75542" ], "nt": "ATGAACCGCGTTTTTTTATTTGGTAAGCTCTCGTTTGATCCTAATAAATTGCAAACCCGTACCAACAATATTGGCGCGTCCTTTTCCTTAGCGTGCATTGATTCGAGCGGTTTTAATGACAGTAAGTCCTATATTAGGATTACTGCTTGGGGTAAAGTTGCTAGCTTTGTTTTAACTTTAAAACCCGGTGACAGTGTTTTTGTGGAGGGGAGATTGTCCACTTACAAGATGAACAACCGCAGTGATGATCCCAATTCGAAGGCCACTTACGCGCTCCAAGTTATCGCTGATAAGGTTTACCGTCCCGATGAGGAAAACAGTTTAGAGCAACCTGTTGATAAGGCTACGGTAATTGATTCACCCTTTTTAGCAGCGAAAACCAATGCTACTGAAAATGAATTAGCCCAAGCATTTCCGATTAGTCTGGATGATGAAGATGATGACATTAACCCCATTTTAAACAACGATTCCCAACTAGAGGAGGAGAGTGATGATGAATAA", "aa": "MNRVFLFGKLSFDPNKLQTRTNNIGASFSLACIDSSGFNDSKSYIRITAWGKVASFVLTLKPGDSVFVEGRLSTYKMNNRSDDPNSKATYALQVIADKVYRPDEENSLEQPVDKATVIDSPFLAAKTNATENELAQAFPISLDDEDDDINPILNNDSQLEEESDDE", "aa_hexdigest": "b6ac7ad7b813f9741825c5f23f1a44b2", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000135FAD", "ncbi_nrp_id": "WP_010874586.1", "uniref100_id": "UniRef100_P75542", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000135FAD", "RefSeq:WP_010874586.1", "UniRef:UniRef100_P75542" ] }, "ips": { "uniref100_id": "UniRef100_P75542", "uniref90_id": "UniRef90_P75542" }, "psc": { "uniref90_id": "UniRef90_P75542", "gene": "ssb", "product": "Single-stranded DNA-binding protein", "uniref50_id": "UniRef50_P75542", "cog_id": "COG0629", "cog_category": "L", "go_ids": [ "GO:0009295", "GO:0003697", "GO:0006260" ] }, "pscc": { "uniref50_id": "UniRef50_P75542", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75542" ], "product": "Single-stranded DNA-binding protein" }, "genes": [ "ssb" ], "id": "APKECPOBEN_531", "locus": "GCA002090215_00495" }, { "type": "cds", "sequence": "contig_1", "start": 280893, "stop": 281207, "strand": "+", "frame": 3, "gene": "rpsR", "product": "30S ribosomal protein S18", "db_xrefs": [ "COG:COG0238", "COG:J", "GO:0000314", "GO:0003735", "GO:0006412", "GO:0022627", "GO:0070181", "KEGG:K02963", "RefSeq:WP_010874587.1", "SO:0001217", "UniParc:UPI0000134DA9", "UniRef:UniRef100_P75541", "UniRef:UniRef50_P47338", "UniRef:UniRef90_P75541" ], "nt": "ATGATGAATAACGAACACGATAATTTCCAAAAAGAAGTTGAAACTACAACTGAAACAACCTTCAACAGAGAAGAAGGCAAAAGAATGGTACGTCCTTTATTTAAGCGTTCTAAAAAGTACTGCCGTTTTTGTGCCATTGGTCAACTTAGAATTGACTTAATTGATGATTTAGAAGCGTTAAAGCGTTTCTTGAGTCCCTACGCTAAGATTAACCCACGCCGGATTACTGGTAACTGCCAAATGCATCAGCGCCATGTAGCCAAGGCCTTAAAGCGTGCACGTTACTTAGCCTTAGTTCCGTTTGTAAAAGATTAG", "aa": "MMNNEHDNFQKEVETTTETTFNREEGKRMVRPLFKRSKKYCRFCAIGQLRIDLIDDLEALKRFLSPYAKINPRRITGNCQMHQRHVAKALKRARYLALVPFVKD", "aa_hexdigest": "13d5ab5bae3dc0a1963c684bbcdb0aa6", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000134DA9", "ncbi_nrp_id": "WP_010874587.1", "uniref100_id": "UniRef100_P75541", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000134DA9", "RefSeq:WP_010874587.1", "UniRef:UniRef100_P75541" ] }, "ips": { "uniref100_id": "UniRef100_P75541", "uniref90_id": "UniRef90_P75541" }, "psc": { "uniref90_id": "UniRef90_P75541", "gene": "rpsR", "product": "30S ribosomal protein S18", "uniref50_id": "UniRef50_P47338", "cog_id": "COG0238", "cog_category": "J", "kegg_orthology_id": "K02963", "go_ids": [ "GO:0000314", "GO:0003735", "GO:0006412", "GO:0022627", "GO:0070181" ] }, "pscc": { "uniref50_id": "UniRef50_P47338", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47338" ], "product": "Small ribosomal subunit protein bS18" }, "genes": [ "rpsR" ], "id": "APKECPOBEN_532", "locus": "GCA002090215_00496" }, { "type": "cds", "sequence": "contig_1", "start": 281210, "stop": 281659, "strand": "+", "frame": 2, "gene": "rplI", "product": "50S ribosomal protein L9", "db_xrefs": [ "COG:COG0359", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904", "KEGG:K02939", "RefSeq:WP_010874588.1", "SO:0001217", "UniParc:UPI00001341F3", "UniRef:UniRef100_P75540", "UniRef:UniRef50_P75540", "UniRef:UniRef90_P75540" ], "nt": "ATGAAGGTAATTTTGAAGCAAGATGTTAGCAACCTAGGCAAACGCTTTGATGTGGTTGATGTTAAAGATGGCTATGCAATCCACTTTTTGTTTCCTAAAAAACTGGCTGCACCTTTAACGAAAAAGAGTTTACAAGACCGTGATTTGTTCTTAAAGAAACAACAAGAACACTATGAGATTAACAAGGCGTTAAGCCACAAGTTAAAGGAAGTAATTGAACAAACAGAACTCCACTTTAGCTTAAAGGAGCACAACGGTCGGCCGTATGGCTCGATTATTACCAAGCAAATTATTAACCAAGCCCACACTAAAGGGATGGCGCTGCAAAAGTTTATGTTTAAAGACAACGTGCGCTTAGGGTTTGGGGATCACGAGATTACCTTACACATCTTTGAAGATACAACCGCAGTTTTAAAAGTTAAGGTAACCCCGGATAATGGTGTCAAATAA", "aa": "MKVILKQDVSNLGKRFDVVDVKDGYAIHFLFPKKLAAPLTKKSLQDRDLFLKKQQEHYEINKALSHKLKEVIEQTELHFSLKEHNGRPYGSIITKQIINQAHTKGMALQKFMFKDNVRLGFGDHEITLHIFEDTTAVLKVKVTPDNGVK", "aa_hexdigest": "d5dbdcbeda3b0120f27d9dcdc51fef08", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001341F3", "ncbi_nrp_id": "WP_010874588.1", "uniref100_id": "UniRef100_P75540", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001341F3", "RefSeq:WP_010874588.1", "UniRef:UniRef100_P75540" ] }, "ips": { "uniref100_id": "UniRef100_P75540", "uniref90_id": "UniRef90_P75540" }, "psc": { "uniref90_id": "UniRef90_P75540", "gene": "rplI", "product": "50S ribosomal protein L9", "uniref50_id": "UniRef50_P75540", "cog_id": "COG0359", "cog_category": "J", "kegg_orthology_id": "K02939", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_P75540", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75540" ], "product": "Large ribosomal subunit protein bL9" }, "genes": [ "rplI" ], "id": "APKECPOBEN_533", "locus": "GCA002090215_00497" }, { "type": "cds", "sequence": "contig_1", "start": 281646, "stop": 282281, "strand": "+", "frame": 3, "gene": "dnaB", "product": "Replicative DNA helicase DnaB", "db_xrefs": [ "COG:COG0305", "COG:L", "EC:5.6.2.3", "GO:0003677", "GO:0003689", "GO:0005524", "GO:0005829", "GO:0006268", "GO:0006269", "GO:0009378", "GO:0016853", "GO:0016887", "GO:0036121", "GO:0061749", "GO:0061775", "GO:0140584", "GO:0140665", "GO:0140849", "GO:1990077", "GO:1990518", "KEGG:K02314", "SO:0001217", "UniRef:UniRef50_P47340", "UniRef:UniRef90_P75539" ], "nt": "ATGGTGTCAAATAAGCTTGAACAGACCTTTAAGTTTGCCAACGACAAGAACATTGAACGTGCTGAGCGTGTTTTAATGCAAGCTTTAGTACAAGATGCCGTTGGTGTGGACTTAATCTTAACCAAGCTTGAACCGAAGGACTTCTTTGGTTTTCCGTTTAACTTTATCTTTCAAACAGCACAGGAAAACTATTCTGAGGGCAATAAGTTGTTTGGTTCGGCGCTTTTAGAGGCCGTTAAGTTTAAGCTGGATACCGATTCCAAAACACAAAGGGAAATTGAAGGTTTATTTGAGGACGTTTTACTCAAAGGTCTTCCCTTTTTGGAAAGGGACATCAAGGTCTTTGTTGATGTTGTCAAAAAAGCTTCGATTTTTCGCCAACTTAAACAGTTCGCCAAAAAGGTTGAAAAGGAAGAGTTTAAGGTCAAAGATGACCGTTTTGAGGGCTATTTACAAGCAATTCAAAACGACTTTACCAAGATTATTCACAGCGCCTTTGTTTCTATTCCCGGTTTGAGTTATGACGAAATTGGTCGTGAAGAGGAAGAGTTAATTCGCAAAGTGTACCGTGGTGAATTAACTGTAAAAGGGCTTAAAAGTGGTTTTTACCCTTTAGATCAATTAACCTCTGGTTGA", "aa": "MVSNKLEQTFKFANDKNIERAERVLMQALVQDAVGVDLILTKLEPKDFFGFPFNFIFQTAQENYSEGNKLFGSALLEAVKFKLDTDSKTQREIEGLFEDVLLKGLPFLERDIKVFVDVVKKASIFRQLKQFAKKVEKEEFKVKDDRFEGYLQAIQNDFTKIIHSAFVSIPGLSYDEIGREEEELIRKVYRGELTVKGLKSGFYPLDQLTSG", "aa_hexdigest": "f06d5a9e348bb88eb54d786ec7ffaa2e", "start_type": "ATG", "rbs_motif": null, "genes": [ "dnaB" ], "truncated": "3-prime", "pseudogene": { "start": 281646, "stop": 282584, "observations": { "mutation": [ 282279 ], "stop": [ 282279 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75539", "query_cov": 1.0, "subject_cov": 0.44608879492600423, "identity": 0.991, "score": 400.0, "evalue": 4.83e-137, "gene_start": 1, "gene_end": 211, "reference_start": 1, "reference_end": 211, "reference_sequence": "MVSNKLEQTFKFANDKNIERAERVLMQALVQDAVGVDLILTKLEPKDFFGFPFNFIFQTAQENYSEGNKLFGSALLEAVKFKLDTDSKTQREIEGLFEDVLLKGLPFLERDIKVFVDVVKKASIFRQLKQFAKKVEKEEFKVKDDRFEGYLQAIQNDFTKIIHSAFVSIPGLSYDEIVREEEELIPKVYRGELTVKGLKSGFYPLDQLTSGWKEGELIVVAARPGRGKTALLINFLQGAVNDSEKFDKNKDVLLFFSLEMRNREIYQRHLMLESQVNYTLTSRQRLANVYDDLIKASEVLRDLPIKIFDYSTITLDEIRAQITEVSKTNNVKLVVIDYLQLVNAFKNSYNISRQQEVTMISKSLKSFAKEFNVPIIAAAQLSRRIEERKDSRPILSDLRESGSIEQDADMVLFIHRVKDEDSENQEAIGHNNIFQVELILEKNRSGPTGKVQFDFQSDVSTFRVREYNPDGYS" }, "paralog": false, "identity": 0.9903846153846154, "score": 597.0, "evalue": 1.65e-216, "description": "Internal stop codon at 282279. Nonsense mutation around 282279.", "psc": { "uniref90_id": "UniRef90_P75539", "gene": "dnaB", "product": "Replicative DNA helicase DnaB", "ec_ids": [ "5.6.2.3" ], "uniref50_id": "UniRef50_P47340", "cog_id": "COG0305", "cog_category": "L", "kegg_orthology_id": "K02314", "go_ids": [ "GO:0005829", "GO:1990077", "GO:0005524", "GO:0016887", "GO:0140849", "GO:0140665", "GO:0140584", "GO:0061775", "GO:0003677", "GO:0003689", "GO:0036121", "GO:0061749", "GO:0009378", "GO:0016853", "GO:1990518", "GO:0006269", "GO:0006268" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75539", "EC:5.6.2.3", "UniRef:UniRef50_P47340", "COG:COG0305", "COG:L", "KEGG:K02314", "GO:0005829", "GO:1990077", "GO:0005524", "GO:0016887", "GO:0140849", "GO:0140665", "GO:0140584", "GO:0061775", "GO:0003677", "GO:0003689", "GO:0036121", "GO:0061749", "GO:0009378", "GO:0016853", "GO:1990518", "GO:0006269", "GO:0006268" ] }, "pscc": { "uniref50_id": "UniRef50_P47340", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47340" ], "product": "Replicative DNA helicase DnaB" } }, "id": "APKECPOBEN_534", "locus": "GCA002090215_00498" }, { "type": "cds", "sequence": "contig_1", "start": 282300, "stop": 283067, "strand": "+", "frame": 3, "gene": "dnaB", "product": "Replicative DNA helicase DnaB", "db_xrefs": [ "COG:COG0305", "COG:L", "EC:5.6.2.3", "GO:0003677", "GO:0003689", "GO:0005524", "GO:0005829", "GO:0006268", "GO:0006269", "GO:0009378", "GO:0016853", "GO:0016887", "GO:0036121", "GO:0061749", "GO:0061775", "GO:0140584", "GO:0140665", "GO:0140849", "GO:1990077", "GO:1990518", "KEGG:K02314", "SO:0001217", "UniRef:UniRef50_P47340", "UniRef:UniRef90_P75539" ], "nt": "GTGGTAGCAGCGCGTCCAGGTCGCGGGAAAACAGCGCTTCTAATTAACTTTTTGCAGGGCGCGGTTAATGACAGCGAAAAGTTTGATAAAAACAAGGATGTGTTGCTGTTTTTTAGCTTAGAAATGCGTAACCGTGAAATTTATCAGCGCCATTTAATGCTAGAAAGCCAGGTAAATTACACACTCACGAGTCGTCAAAGGCTCGCTAATGTGTATGATGATTTAATTAAAGCATCAGAAGTATTGCGCGATCTTCCTATTAAAATTTTTGATTACAGCACGATTACGTTGGACGAAATTCGTGCTCAAATCACGGAAGTTTCAAAAACCAATAACGTTAAGTTGGTCGTAATTGACTATCTCCAACTAGTTAATGCATTTAAAAACAGCTATAACATTTCGCGTCAACAAGAGGTCACCATGATTTCCAAATCACTTAAAAGCTTTGCCAAGGAGTTTAACGTACCAATTATTGCCGCTGCACAGCTTTCTCGAAGAATAGAAGAACGCAAAGATTCGCGTCCCATTTTGTCGGATTTACGTGAATCGGGTTCGATTGAACAGGATGCTGACATGGTTTTATTCATCCACCGCGTCAAGGATGAGGACAGTGAAAATCAAGAAGCAATTGGTCACAACAATATTTTTCAAGTGGAGTTAATCTTGGAAAAAAACCGTAGTGGTCCGACTGGTAAAGTACAGTTTGACTTTCAAAGTGACGTTTCTACCTTTCGGGTTCGGGAATACAATCCTGATGGTTACAGTTAG", "aa": "MVAARPGRGKTALLINFLQGAVNDSEKFDKNKDVLLFFSLEMRNREIYQRHLMLESQVNYTLTSRQRLANVYDDLIKASEVLRDLPIKIFDYSTITLDEIRAQITEVSKTNNVKLVVIDYLQLVNAFKNSYNISRQQEVTMISKSLKSFAKEFNVPIIAAAQLSRRIEERKDSRPILSDLRESGSIEQDADMVLFIHRVKDEDSENQEAIGHNNIFQVELILEKNRSGPTGKVQFDFQSDVSTFRVREYNPDGYS", "aa_hexdigest": "ecb9c031c3a78514b207c1530bb8a05d", "start_type": "GTG", "rbs_motif": "AAAA", "genes": [ "dnaB" ], "truncated": "5-prime", "pseudogene": { "start": 282000, "stop": 283067, "observations": { "mutation": [ 282279 ], "stop": [ 282279 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75539", "query_cov": 1.0, "subject_cov": 0.5391120507399577, "identity": 0.996, "score": 485.0, "evalue": 1.61e-169, "gene_start": 1, "gene_end": 255, "reference_start": 219, "reference_end": 473, "reference_sequence": "MVSNKLEQTFKFANDKNIERAERVLMQALVQDAVGVDLILTKLEPKDFFGFPFNFIFQTAQENYSEGNKLFGSALLEAVKFKLDTDSKTQREIEGLFEDVLLKGLPFLERDIKVFVDVVKKASIFRQLKQFAKKVEKEEFKVKDDRFEGYLQAIQNDFTKIIHSAFVSIPGLSYDEIVREEEELIPKVYRGELTVKGLKSGFYPLDQLTSGWKEGELIVVAARPGRGKTALLINFLQGAVNDSEKFDKNKDVLLFFSLEMRNREIYQRHLMLESQVNYTLTSRQRLANVYDDLIKASEVLRDLPIKIFDYSTITLDEIRAQITEVSKTNNVKLVVIDYLQLVNAFKNSYNISRQQEVTMISKSLKSFAKEFNVPIIAAAQLSRRIEERKDSRPILSDLRESGSIEQDADMVLFIHRVKDEDSENQEAIGHNNIFQVELILEKNRSGPTGKVQFDFQSDVSTFRVREYNPDGYS" }, "paralog": false, "identity": 0.9915492957746479, "score": 677.0, "evalue": 2.11e-247, "description": "Internal stop codon at 282279. Nonsense mutation around 282279.", "psc": { "uniref90_id": "UniRef90_P75539", "gene": "dnaB", "product": "Replicative DNA helicase DnaB", "ec_ids": [ "5.6.2.3" ], "uniref50_id": "UniRef50_P47340", "cog_id": "COG0305", "cog_category": "L", "kegg_orthology_id": "K02314", "go_ids": [ "GO:0005829", "GO:1990077", "GO:0005524", "GO:0016887", "GO:0140849", "GO:0140665", "GO:0140584", "GO:0061775", "GO:0003677", "GO:0003689", "GO:0036121", "GO:0061749", "GO:0009378", "GO:0016853", "GO:1990518", "GO:0006269", "GO:0006268" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75539", "EC:5.6.2.3", "UniRef:UniRef50_P47340", "COG:COG0305", "COG:L", "KEGG:K02314", "GO:0005829", "GO:1990077", "GO:0005524", "GO:0016887", "GO:0140849", "GO:0140665", "GO:0140584", "GO:0061775", "GO:0003677", "GO:0003689", "GO:0036121", "GO:0061749", "GO:0009378", "GO:0016853", "GO:1990518", "GO:0006269", "GO:0006268" ] }, "pscc": { "uniref50_id": "UniRef50_P47340", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47340" ], "product": "Replicative DNA helicase DnaB" } }, "id": "APKECPOBEN_535", "locus": "GCA002090215_00499" }, { "type": "cds", "sequence": "contig_1", "start": 283071, "stop": 283481, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGTACAAAACAGTTAAATCGATCCCGCTTTTTTTGTTAGGCTCGATTGTGTTCACTGCTTGTTCAACACCACAATCAACCTTTCATTTACCTGTGCAAACGACCGTTAGCGCAATCAAAAAAGATATTAGTGGGAAAACAGCTACTGCTGTTAAGGCTGCTAGTAGTTCATCTAGTACTACTACATCTAACGATGACAACAACCAAAAGGGTTACTTCTTAGAAACTAATCGTTCCACCGGTACTTACGACCCTAACAATTCCACCCGGTTAATTAAATTAGGTGAAAGTGGTGATTTTCATGCAGCTGACCAGAATAAACCGGAAGAAGCCTTGTTTGAACGACTTTACGGCGGCATTGCTTCCTTATTAAATTTCCGCATTATTAAACCTGCTTTAACTTATTGA", "aa": "MKYKTVKSIPLFLLGSIVFTACSTPQSTFHLPVQTTVSAIKKDISGKTATAVKAASSSSSTTTSNDDNNQKGYFLETNRSTGTYDPNNSTRLIKLGESGDFHAADQNKPEEALFERLYGGIASLLNFRIIKPALTY", "aa_hexdigest": "3c9bf81ed1388f87208e723ee440fe3a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14759.450199999985, "isoelectric_point": 9.239302635192868 }, "id": "APKECPOBEN_536", "locus": "GCA002090215_00500" }, { "type": "cds", "sequence": "contig_1", "start": 283575, "stop": 284201, "strand": "+", "frame": 3, "gene": null, "product": "putative lipo MG095-like protein", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P75538", "UniRef:UniRef90_P75538" ], "nt": "TTGGCTAATAACTTAATTGTCGCTGATGATGGCAACAACAACTTTTGGTTTGGTTTAAAGTCACTTAGCTTTAATGCAGCTAAGTTGACTGACAACGCCCAAACACAAATGGCGCAAAAAACCACTCAAGCAGTTACCTTGAAAAGTCAAGCGCAAATGTCAAGTACAAATACCAAAAACACCAACAAAAAGATTGATTTGCGCGATAAGATTACTTTATCGAGTACGATGAATACTCAAGGAAGTGGTGATAACAAAAACCCATCTAGTGGTCTTATTCAAAAGTTAGTGAGTGTCGAAAATATTGAAGCTGAATTTAGTTTTGTTAAAACTGGCTTCAACGGTAATGAAATTAAATTTGGCGATTTTGTAACTGAGAATTCACCCACAACCACTCAACTTAAGCAAGTTTGGAAAAAGAAGTGGGGCACGGAATTGAAAAAGACAAACTATAAGCTTCAATTGAACAACTTTAGTCTATTGCTAACTTACACACCAGAAGTTAATAAAGTTGAAAAAGGTAACAATGGTGATTCCAATAAAGGCACGATCGCTACGCCAAACGGTTTTAGCTTCTTGTACCCAGCTAATTTAAATGAGACACCAAGCAGTAGCAGTAGTTATTGA", "aa": "MANNLIVADDGNNNFWFGLKSLSFNAAKLTDNAQTQMAQKTTQAVTLKSQAQMSSTNTKNTNKKIDLRDKITLSSTMNTQGSGDNKNPSSGLIQKLVSVENIEAEFSFVKTGFNGNEIKFGDFVTENSPTTTQLKQVWKKKWGTELKKTNYKLQLNNFSLLLTYTPEVNKVEKGNNGDSNKGTIATPNGFSFLYPANLNETPSSSSSY", "aa_hexdigest": "97a9ad0ae91ad0978617427e62821b39", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 283278, "stop": 284435, "observations": { "mutation": [ 283479, 284199 ], "stop": [ 283479, 284199 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75538", "query_cov": 1.0, "subject_cov": 0.4581497797356828, "identity": 0.99, "score": 395.0, "evalue": 3.06e-135, "gene_start": 1, "gene_end": 208, "reference_start": 169, "reference_end": 376, "reference_sequence": "MKYKTVKSIPLFLLGSIVFTACSTPQSTFHLPVQTTVSAIKKDISGKTATAVKAASSSSSTTTSNDDNNQKGYFLETNRSTGTYDPNNSTRLIKLGESGDFHAADQNKPEEALFERLYGGIASLLNFRIIKPALTYWNTVTPSLKAIGKSSNLITFSQDIDETELQRALANNLIVADDGNNNFWFGLKSLSFNSAKLTDNAQTQMAQKTTQAVTLKSQAQMSSTNTKNTNKKIDLRDKITLSSTMNTQGSGDNKNPSSGLIQKLVSVENIEAEFSFVKTGFNGNEIKFGDFVTENSPTTTQLKQVWKKKWGTELKKTNYKLQLNNFSLLLTYTPEVNKVEKGNNGDSNKGTIATPNGFSFLYPANLNETPSSSSSYWTNVTDLTKAATDTENTNLLNDLQKSQEQVNQFVAAITQNHLDVSEAALTKKQFGSLSISDFFKAIFKENGKDTKAKS" }, "paralog": false, "identity": 0.9922077922077922, "score": 751.0, "evalue": 9.63e-278, "description": "Internal stop codon at 283479, 284199. Nonsense mutation around 283479, 284199.", "psc": { "uniref90_id": "UniRef90_P75538", "product": "Uncharacterized lipoprotein MG095 homolog", "uniref50_id": "UniRef50_P75538", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75538", "UniRef:UniRef50_P75538", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75538", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75538" ], "product": "Uncharacterized lipoprotein MG095 homolog" } }, "id": "APKECPOBEN_537", "locus": "GCA002090215_00501" }, { "type": "cds", "sequence": "contig_1", "start": 284265, "stop": 284435, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAAAAGAGTCAGGAACAAGTCAATCAATTTGTTGCTGCTATTACCCAAAATCACCTCGATGTTTCCGAAGCTGCTTTAACTAAAAAACAGTTTGGTAGCCTTAGCATTAGTGATTTCTTTAAAGCCATCTTTAAGGAAAATGGCAAAGACACTAAAGCTAAAAGTTAA", "aa": "MQKSQEQVNQFVAAITQNHLDVSEAALTKKQFGSLSISDFFKAIFKENGKDTKAKS", "aa_hexdigest": "2b4ec1a1cb8e74bb232977eaeff3cd23", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6233.969299999999, "isoelectric_point": 9.158652687072756 }, "id": "APKECPOBEN_538", "locus": "GCA002090215_00502" }, { "type": "cds", "sequence": "contig_1", "start": 284863, "stop": 285906, "strand": "+", "frame": 1, "gene": null, "product": "putative protein MPN_234", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75537", "UniRef:UniRef90_P75537" ], "nt": "TTGACTGGTGTACTTGAAACAATTCCCCAACTGCAAAAGGAAATAAAAGCTGCTAATGACATTTTCCCTGTCAATGAACAAAGTCAAATTCCCCGTGTAGAAGCACGCTTATTTGACTTTTTGGGCAGTGGCTTTTTCCCGGTATTGAACCCGAAAGGTTTAGGGTTAGCGGACAACGTTGCTAGTTTGTTTGAACAGTTTCAGCTAGCATCAATAAACTTAAGTGGTTTTGAAGTCAAACTGATTAAAACGCATGACATTGTCATTAAGAGTAAGGTACGTTACAGCTTTGAAGTGCAAATGCAGTTCCATGCTGTTTACCAAAATAACGACACTAAAGCTAATTTAGATTTTGCGCTCAATATTAGTACGATTAACTTCGCTACCTTACAAGAATTACAAAACAGTTTTGATCTTCAAGGTAGTGATTTAACGGCTGGGTTGTTTTACAAATACAGCGTTAATAAGTTAACTAGTGGTACTAACGATTTAACCACAATTGCTAAAACCGCTTTAGGTGAAAACATTATCCAAAAGCAAGTGAGCCTAACACAATCAATTATTAAGCCACGCCTAGAAGCTGCAAAAACTCAGTACAAACAGGATATTATTGCACCTTTTGCAAAGGAACGTCAAGCTGCTTTGGCACAACATTTAAAAGAAATAGAAGAGGCCAAACAACGTGCCGAGCAATTATTAAAAGAACAGCAAGAAGCGGAAAAACGGCGCCAAGAAGAAGTTAAGAATGTCGCAGAAACACAACAGTTTAATGATAGCTTAACTTCAGCACAAAAATTTAAAGAGTACTGGTTAAAACAAGGTAAGGATGTTACCAAAAAGGTTGAACTCATTCAGGCGCTGAAATCATCTTTCTTCCGCAACCAAAACCGTACCTTTAACTTTTTAATTGCCGGTTTTCGAACTGCCATTGATTGGTACTACAATCAAGAGAAAAATAATACCACTGCTAAAAATAACGCTTTTGGTAAGAATGGCATTCAGTTCCCTGTGGCTGGCTTTCAGGGAATTTACATGTCACAGTGA", "aa": "MTGVLETIPQLQKEIKAANDIFPVNEQSQIPRVEARLFDFLGSGFFPVLNPKGLGLADNVASLFEQFQLASINLSGFEVKLIKTHDIVIKSKVRYSFEVQMQFHAVYQNNDTKANLDFALNISTINFATLQELQNSFDLQGSDLTAGLFYKYSVNKLTSGTNDLTTIAKTALGENIIQKQVSLTQSIIKPRLEAAKTQYKQDIIAPFAKERQAALAQHLKEIEEAKQRAEQLLKEQQEAEKRRQEEVKNVAETQQFNDSLTSAQKFKEYWLKQGKDVTKKVELIQALKSSFFRNQNRTFNFLIAGFRTAIDWYYNQEKNNTTAKNNAFGKNGIQFPVAGFQGIYMSQ", "aa_hexdigest": "9cf2a8e6f1af61ca881d13d8d0705a27", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 284563, "stop": 286209, "observations": { "mutation": [ 284752, 284851, 285904, 286096, 286138 ], "stop": [ 284752, 284851, 285904, 286096, 286138 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75537", "query_cov": 1.0, "subject_cov": 0.5481832543443917, "identity": 0.997, "score": 630.0, "evalue": 8.41e-223, "gene_start": 1, "gene_end": 347, "reference_start": 117, "reference_end": 463, "reference_sequence": "MKFPKKRWQKWAFLTGSFTVLPTLIAFFSILPHSSVLEPSHLRFNSTSQRYQSDNSHLLNLHGEAFSDADLNAVAEHTDWDANFALFQQKFKTKPITVNQLGLVDVFNLLSGWKQSLTGVLETIPQLQKEIKAANDIFPVNEQSQIPRVEARLFDFLGSGFFPVLNPKGLGLADNVASLFEQFQLASINLSGFEVKLIKTHDIVIKSKVRYSFEVQMQFHAVYQNNDTKANLDFALNISTINFATLQELQNSFDLQGSDLTAGLFYKYSVNKLTSGTNDLTTIAKTALGENIIQKQVSLTQSIIKPRLEAAKTQYKQDIIAPFAKERQAALAQHLKEIEEAKQRAEQLLKEQQEAEKRRQEEVKNVAETQQFNDSLTSAQKFKEYWLKQGKDVTKKVELIQALKSSFFRNQNRTFNFLIAGFRTAIDWYYNQEKNNTTAKNNAFGKNGIQFPVAGFQGIYMSQWLRDELSGKTDIKLNLKSLSVQNENKNSSINWNKQKRIEIKQVKPFNYSFEINLKYTGSYNVSLWYLIGAAIGGIPTSWSGTMDMKFIVDGDLDSGIVTKQDYPGSKFEFTEDKLWFTLHVKQQIKVKEQGFMNLLKGQSLDNLDLRTGTTKPPVVDLASYLHFVILTAK" }, "paralog": false, "identity": 0.9908759124087592, "score": 1021.0, "evalue": 0.0, "description": "Internal stop codon at 284752, 284851, 285904, 286096, 286138. Nonsense mutation around 284752, 284851, 285904, 286096, 286138.", "psc": { "uniref90_id": "UniRef90_P75537", "product": "Uncharacterized protein MPN_234", "uniref50_id": "UniRef50_P75537", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75537", "UniRef:UniRef50_P75537" ] }, "pscc": { "uniref50_id": "UniRef50_P75537", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75537" ], "product": "Uncharacterized protein MPN_234" } }, "id": "APKECPOBEN_539", "locus": "GCA002090215_00503" }, { "type": "cds", "sequence": "contig_1", "start": 285907, "stop": 286098, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03072.19" ], "nt": "TTGCGTGACGAGCTCTCTGGTAAGACCGACATTAAGCTCAACTTAAAAAGTTTAAGTGTTCAGAATGAAAATAAAAATTCTTCCATTAATTGGAACAAGCAAAAAAGAATTGAGATTAAACAAGTAAAACCATTTAACTATTCTTTTGAAATTAACTTGAAGTACACTGGTAGCTATAATGTCAGTCTTTGA", "aa": "MRDELSGKTDIKLNLKSLSVQNENKNSSINWNKQKRIEIKQVKPFNYSFEINLKYTGSYNVSL", "aa_hexdigest": "b220fc0c1ec9eeda9cb2e3f0537a031c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03072.19", "name": "DUF237", "length": 70, "aa_cov": 0.9841269841269841, "hmm_cov": 1.0, "evalue": 6.850819243468526e-19, "score": 63.76200866699219, "start": 1, "stop": 62 } ], "seq_stats": { "molecular_weight": 7395.322900000001, "isoelectric_point": 9.697672843933105 }, "id": "APKECPOBEN_540", "locus": "GCA002090215_00504" }, { "type": "cds", "sequence": "contig_1", "start": 286150, "stop": 286416, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03072.19" ], "nt": "ATGGATATGAAGTTTATCGTTGATGGTGATTTGGATAGCGGTATTGTTACCAAGCAAGATTATCCCGGTTCTAAGTTTGAATTTACCGAAGACAAACTGTGGTTTACCCTGCACGTTAAACAACAAATTAAAGTTAAAGAGCAGGGCTTTATGAACTTGCTTAAGGGTCAAAGTTTGGACAACTTGGATCTACGCACTGGTACAACTAAACCGCCAGTAGTTGATTTAGCGAGCTACCTCCACTTTGTTATTTTGACAGCTAAGTAG", "aa": "MDMKFIVDGDLDSGIVTKQDYPGSKFEFTEDKLWFTLHVKQQIKVKEQGFMNLLKGQSLDNLDLRTGTTKPPVVDLASYLHFVILTAK", "aa_hexdigest": "26088d073aaa33af3526bbdd31353688", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03072.19", "name": "DUF237", "length": 48, "aa_cov": 0.5340909090909091, "hmm_cov": 1.0, "evalue": 4.9907940403841335e-18, "score": 60.96890640258789, "start": 1, "stop": 47 } ], "seq_stats": { "molecular_weight": 10034.49619999999, "isoelectric_point": 6.040245628356933 }, "id": "APKECPOBEN_541", "locus": "GCA002090215_00505" }, { "type": "cds", "sequence": "contig_1", "start": 286441, "stop": 286782, "strand": "+", "frame": 1, "gene": "ung", "product": "uracil-DNA glycosylase", "db_xrefs": [ "COG:COG0692", "COG:L", "EC:3.2.2.27", "GO:0004844", "GO:0005737", "GO:0006281", "GO:0006284", "GO:0097510", "KEGG:K03648", "SO:0001217", "UniRef:UniRef50_P75536", "UniRef:UniRef90_P75536" ], "nt": "ATGGAACAACTTTTAGACCAGTTTTTAAGTGGTGTTTTAAGCGAATGGAAGGCTTTTATTTTGCAAGAAGCCAAACAGACCTATTTTCAAGAATTGTTAGCTAAACTAAAAAATGTCGAGCAAGAGCTAACTCCCAAAGCCTCTCAAGTCTTTCGTCCCTTTAGCTTTTTTGCACCAAATAACACTAAACTAATTATTTACGGTCAGGATCCATATCCTAATCCACAACACGCTTGTGGTCTTTCGTTTGCCTCTAACGCCCCTAAACTACCACAAAGCTTAAAGCGGATGATTCTGCGCTTGCAGCAAGAGTATCCGGAACTAGCAGGTCAAAATCACTGA", "aa": "MEQLLDQFLSGVLSEWKAFILQEAKQTYFQELLAKLKNVEQELTPKASQVFRPFSFFAPNNTKLIIYGQDPYPNPQHACGLSFASNAPKLPQSLKRMILRLQQEYPELAGQNH", "aa_hexdigest": "49a9ea9bbb4c4529cd72f5f243a6f61a", "start_type": "ATG", "rbs_motif": null, "genes": [ "ung" ], "truncated": "3-prime", "pseudogene": { "start": 286441, "stop": 287085, "observations": { "mutation": [ 286780, 286804, 286885 ], "stop": [ 286780, 286804, 286885 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75536", "query_cov": 1.0, "subject_cov": 0.4708333333333333, "identity": 1.0, "score": 226.0, "evalue": 1.96e-73, "gene_start": 1, "gene_end": 113, "reference_start": 1, "reference_end": 113, "reference_sequence": "MEQLLDQFLSGVLSEWKAFILQEAKQTYFQELLAKLKNVEQELTPKASQVFRPFSFFAPNNTKLIIYGQDPYPNPQHACGLSFASNAPKLPQSLKRMILRLQQEYPELAGQNHWTKQLLEGWAQQGILLLNGVFTTNAFQTNAHRNWGWEQFNCHLLDFLLSQKLYVLLVFLGKQTENFVLKKIGGASQFASLSYPHPSPLTGRKFFDHPDALFKQINQWLKHHNHTPIDWTNGQVQYQF" }, "paralog": false, "identity": 0.985981308411215, "score": 425.0, "evalue": 4.22e-154, "description": "Internal stop codon at 286780, 286804, 286885. Nonsense mutation around 286780, 286804, 286885.", "psc": { "uniref90_id": "UniRef90_P75536", "gene": "ung", "product": "uracil-DNA glycosylase", "ec_ids": [ "3.2.2.27" ], "uniref50_id": "UniRef50_P75536", "cog_id": "COG0692", "cog_category": "L", "kegg_orthology_id": "K03648", "go_ids": [ "GO:0004844", "GO:0005737", "GO:0006281", "GO:0006284", "GO:0097510" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75536", "EC:3.2.2.27", "UniRef:UniRef50_P75536", "COG:COG0692", "COG:L", "KEGG:K03648", "GO:0004844", "GO:0005737", "GO:0006281", "GO:0006284", "GO:0097510" ] }, "pscc": { "uniref50_id": "UniRef50_P75536", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75536" ], "product": "Uracil-DNA glycosylase" } }, "id": "APKECPOBEN_542", "locus": "GCA002090215_00506" }, { "type": "cds", "sequence": "contig_1", "start": 287361, "stop": 288326, "strand": "+", "frame": 3, "gene": "gatC", "product": "Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatC", "db_xrefs": [ "COG:COG0721", "COG:J", "GO:0005886", "GO:0006424", "GO:0006450", "GO:0030956", "GO:0050567", "GO:0070681", "SO:0001217", "UniRef:UniRef50_P47344", "UniRef:UniRef90_P75535" ], "nt": "ATGGATAAATTAATAAATAAACCTCAACCATTAACTTCTGATATTTCCACTTCTGGATTTATTTACTTTGCGGTTGTTTTTGTAGCCATTATGGGCTACCTTTTATTCAAAAATCTACTGTTTTTATTTTTCTTTAAACGCTATCCAAAAAACACTCCCAAAATTGGGGTAGGTAACATTACCACGATTGCCATGATCATTGCTGTGGCGGTGTCGATTGTTTTAGTGTTAATGGCACTAGCAGGCGGTTTAGCCGCTGCTTTGTTCCGTGGTTATCCTGGTTTTCGGGTCACCTTGGAGTTAATCCTTGTAAAGATTTCGGGACTGTTGTTTGGTCCAATTGTCGGCATTTTTTCCGCGGCCACAATTGACTTTTTAACGGTCATCTTTTCGGGTGGGGTTTTTAACGTTGGTTATGTTTTAGGTGCCATCTTAACCGGGATGATTGCTGGCATACTGCGTGAAGTTTTAATTTCAACAGCACTTTTACACAACCGTAATTTATCGGATTTTGCCTACCTAGTGCTCTCGATTGGCATGGTCATTGCCGCCTTTTTAATTACCCAGTTCTTTGTGCTTGGGATAAGTAATAACCTTAAGGAGATTAAGGGTGATGAAGAGTTTCGCTTGAAATTTAACGCACCATCAATTGTCTTTGAGCTTTCGTTAACCCAGTACGCCAACATTCTGCTCTACTTTACCATTGCCATAGTTATTGCTATGCTGGTGTTGTACATTGTTTGGTTAGTCAAACAGCGTCACTTAAGTTTTGAGCACTCTCGTTTCTTTTACCGCAGTTACAAACATGCTAATCACCAGTTTACCCTGTTTGTACTAACTAAGGAAAACTGGTTCTATTTAATCTTGAATGTAATCACCTTAGCTTCCACTAGTTTGTTGATGATCAACATTGCCTTCATTCCGATCTTTGACACGCAAACAACTGGTCAAACATACGAGTTTTGA", "aa": "MDKLINKPQPLTSDISTSGFIYFAVVFVAIMGYLLFKNLLFLFFFKRYPKNTPKIGVGNITTIAMIIAVAVSIVLVLMALAGGLAAALFRGYPGFRVTLELILVKISGLLFGPIVGIFSAATIDFLTVIFSGGVFNVGYVLGAILTGMIAGILREVLISTALLHNRNLSDFAYLVLSIGMVIAAFLITQFFVLGISNNLKEIKGDEEFRLKFNAPSIVFELSLTQYANILLYFTIAIVIAMLVLYIVWLVKQRHLSFEHSRFFYRSYKHANHQFTLFVLTKENWFYLILNVITLASTSLLMINIAFIPIFDTQTTGQTYEF", "aa_hexdigest": "5c0aeb3c80db05ed8958c954441d6aee", "start_type": "ATG", "rbs_motif": null, "genes": [ "gatC" ], "truncated": "3-prime", "pseudogene": { "start": 287361, "stop": 288629, "observations": { "mutation": [ 288324 ], "stop": [ 288324 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75535", "query_cov": 1.0, "subject_cov": 0.6701461377870563, "identity": 1.0, "score": 580.0, "evalue": 5.53e-206, "gene_start": 1, "gene_end": 321, "reference_start": 1, "reference_end": 321, "reference_sequence": "MDKLINKPQPLTSDISTSGFIYFAVVFVAIMGYLLFKNLLFLFFFKRYPKNTPKIGVGNITTIAMIIAVAVSIVLVLMALAGGLAAALFRGYPGFRVTLELILVKISGLLFGPIVGIFSAATIDFLTVIFSGGVFNVGYVLGAILTGMIAGILREVLISTALLHNRNLSDFAYLVLSIGMVIAAFLITQFFVLGISNNLKEIKGDEEFRLKFNAPSIVFELSLTQYANILLYFTIAIVIAMLVLYIVWLVKQRHLSFEHSRFFYRSYKHANHQFTLFVLTKENWFYLILNVITLASTSLLMINIAFIPIFDTQTTGQTYEFWLLARLLFAPVIFLLDIIVIYPILLLLTPLMLKGFKTAVSKNQRKTLKQSFTDLQSVVLPIINKRKHQQLRQEELKRLARATHFDLTEGEMEKLLVEFKTITQSFDRVMNIDTTSVEPMYAPFNTSPTPLRKDKVIVEKHPEKLLANCKEMSVGFVKV" }, "paralog": false, "identity": 0.9976303317535545, "score": 805.0, "evalue": 1.03e-296, "description": "Internal stop codon at 288324. Nonsense mutation around 288324.", "psc": { "uniref90_id": "UniRef90_P75535", "gene": "gatC", "product": "Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatC", "uniref50_id": "UniRef50_P47344", "cog_id": "COG0721", "cog_category": "J", "go_ids": [ "GO:0005886", "GO:0006424", "GO:0006450", "GO:0030956", "GO:0050567", "GO:0070681" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75535", "UniRef:UniRef50_P47344", "COG:COG0721", "COG:J", "GO:0005886", "GO:0006424", "GO:0006450", "GO:0030956", "GO:0050567", "GO:0070681" ] }, "pscc": { "uniref50_id": "UniRef50_P47344", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47344" ], "product": "Uncharacterized protein MG098" } }, "id": "APKECPOBEN_543", "locus": "GCA002090215_00507" }, { "type": "cds", "sequence": "contig_1", "start": 288363, "stop": 288800, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTGGACATTATTGTGATCTATCCAATTCTGCTGTTATTAACCCCGCTAATGCTCAAAGGTTTTAAAACAGCAGTTTCTAAAAACCAACGCAAAACACTAAAACAAAGCTTTACTGATTTGCAAAGTGTAGTGTTGCCCATTATTAACAAACGTAAACACCAGCAGTTGCGCCAAGAAGAGTTAAAACGCTTAGCACGAGCCACTCACTTTGATTTGACAGAAGGCGAAATGGAAAAGCTCTTGGTCGAATTTAAAACTATTACCCAGAGCTTTGACCGGGTGATGAACATTGATACCACCAGTGTGGAACCAATGTACGCACCTTTCAATACATCACCAACGCCACTGCGTAAAGATAAGGTAATAGTGGAAAAGCATCCCGAAAAATTATTAGCCAACTGTAAGGAAATGAGTGTTGGCTTTGTCAAAGTATAG", "aa": "MLDIIVIYPILLLLTPLMLKGFKTAVSKNQRKTLKQSFTDLQSVVLPIINKRKHQQLRQEELKRLARATHFDLTEGEMEKLLVEFKTITQSFDRVMNIDTTSVEPMYAPFNTSPTPLRKDKVIVEKHPEKLLANCKEMSVGFVKV", "aa_hexdigest": "b81d3988cf6cbfa4502208105427e717", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16753.69129999999, "isoelectric_point": 9.605740928649901 }, "id": "APKECPOBEN_544", "locus": "GCA002090215_00508" }, { "type": "cds", "sequence": "contig_1", "start": 289055, "stop": 289204, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01425.27" ], "nt": "TTGGAAGACTATATCCCGCCGTTTTCAGCAACTGTGTTTGAATTGCTAAACAACAGTGGCGCTTTATTAGTTGGTAAAGCGAACCTCGACGAGTTTGGTTTAGGCGGTACGGGGCTCCATTCGGGTTTTGGTTTTGTCCACCACCCTTGA", "aa": "MEDYIPPFSATVFELLNNSGALLVGKANLDEFGLGGTGLHSGFGFVHHP", "aa_hexdigest": "1f21a5e9cf2fece19ab7d8f96b820894", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01425.27", "name": "Amidase", "length": 48, "aa_cov": 0.9795918367346939, "hmm_cov": 1.0, "evalue": 7.154958528111946e-13, "score": 43.77743148803711, "start": 2, "stop": 49 } ], "seq_stats": { "molecular_weight": 5174.751499999999, "isoelectric_point": 4.834747505187988 }, "id": "APKECPOBEN_545", "locus": "GCA002090215_00509" }, { "type": "cds", "sequence": "contig_1", "start": 289253, "stop": 289612, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01425.27" ], "nt": "GTGGCGCGCGGCATTGTTCCCTTTTCAATTGGTACGGACACGGGAGATTCGGTACGCCGTCCAGCTAGCATTTGTAACATTGTTGGTTTTAAGCCCACCTATGGTTTGATATCGCGGAACGGAGTGTATCCTTACGCGCCTAGTTTAGACCATGTCGGGATCTTTGCGCGGTATGTGTATGATGTAGCACTTGTTAGTGATGAAATCATTAAACATGACAAAGCTGATTTTTCCGCTCAAAAATCTCCTGATGCTGGCAAGTTTACCAGGTCCTTAAAAGAGTCCTTCAACAAGCAAATCAAGATAGGTTATCTAAAACCGCTAGAGGAATGGTTTGACATTGAACTGAGCAAAAAGTGA", "aa": "MARGIVPFSIGTDTGDSVRRPASICNIVGFKPTYGLISRNGVYPYAPSLDHVGIFARYVYDVALVSDEIIKHDKADFSAQKSPDAGKFTRSLKESFNKQIKIGYLKPLEEWFDIELSKK", "aa_hexdigest": "d40eabf11527c5f792c7ba569a20d339", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01425.27", "name": "Amidase", "length": 113, "aa_cov": 0.9495798319327731, "hmm_cov": 0.9911504424778761, "evalue": 6.027409042412702e-41, "score": 136.30691528320312, "start": 2, "stop": 114 } ], "seq_stats": { "molecular_weight": 13303.072799999987, "isoelectric_point": 8.989681053161618 }, "id": "APKECPOBEN_546", "locus": "GCA002090215_00510" }, { "type": "cds", "sequence": "contig_1", "start": 289637, "stop": 290116, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01425.27" ], "nt": "TTGGAGGGTTGTGAGTTAATTCCGTTTCACTTTCCGCTTGAACTGCTAGAGGTAATTGATCCAGTTTACAAGCTGATTAGTTACAGTGAAGCGGTGAGCTGTTACAGTAATCTCACTGGGATTGTCTTTGGTCAAAAATTGTTTGAACCCAACCAAGCGAGTGACTTTTCCAAAACAATTACTGCCAACCGCGACCGTTTCTTTGGTGAGCAGTTAAAACGCCGCTTTATTATTGGTGCTTTTGGTACTGACAAGAATAACTTTACCAAGTACTTTGAAAAGGCTCAAAAGATCCGCCGGGTAATGGTTGATGCTTACTTAAACCTGTTTAAAGAAGCGGATTTCATTGTGTCACCTAGTGCATCCGGCTTTACTAAAACAATAGCTGCGGTGCAAAAGGGTGAGAGCTTTACTAACCTCGTGGATGACTTTTTACAACTAGCTAACTTTGCTGGTAATCCTTCAATTACGATTCCATGA", "aa": "MEGCELIPFHFPLELLEVIDPVYKLISYSEAVSCYSNLTGIVFGQKLFEPNQASDFSKTITANRDRFFGEQLKRRFIIGAFGTDKNNFTKYFEKAQKIRRVMVDAYLNLFKEADFIVSPSASGFTKTIAAVQKGESFTNLVDDFLQLANFAGNPSITIP", "aa_hexdigest": "dc2a056738c208e9952c47c45cafb867", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01425.27", "name": "Amidase", "length": 162, "aa_cov": 0.9874213836477987, "hmm_cov": 0.9382716049382716, "evalue": 8.338885633222484e-27, "score": 89.70026397705078, "start": 3, "stop": 159 } ], "seq_stats": { "molecular_weight": 17931.30759999999, "isoelectric_point": 5.8598955154418935 }, "id": "APKECPOBEN_547", "locus": "GCA002090215_00511" }, { "type": "cds", "sequence": "contig_1", "start": 290232, "stop": 291665, "strand": "+", "frame": 3, "gene": "gatB", "product": "Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB", "db_xrefs": [ "COG:COG0064", "COG:J", "EC:6.3.5.-", "GO:0005524", "GO:0006412", "GO:0006424", "GO:0016884", "GO:0030956", "GO:0050566", "GO:0050567", "GO:0070681", "KEGG:K02434", "RefSeq:WP_014574916.1", "SO:0001217", "UniParc:UPI000012B157", "UniRef:UniRef100_P75533", "UniRef:UniRef50_P75533", "UniRef:UniRef90_P75533" ], "nt": "ATGATTAACTTTGAAGCAATTATTGGGATTGAGGTACACGTGGTCTTAAACACTGCTACCAAAATGTTTTCCCCAGCGCCCAACCAAGCGCAAAACGCCACACCAAACCAGTTCATAAACGCGATTGATTTGGGCTTGCCTGGTACCATGCCCCAGGTCAATGAGCAGGCGGTACAAAAGGCGTTAATCCTTGCTGACGCGCTCAACATGCAACGGGTACAGCCAGTCTTAGTGTTTGACCGTAAGCACTACTTTTACCAAGATCTACCTAAGGGTTTTCAGATTACCCAGCAAAACTTTCCGATTGCGCAAAACGGTTACGTTGAAATTGTGGAAAACAACAAAGCGCAGCGGATTATCATTGAACGGTTTCACCTAGAGGAAGATACTGCCAAGCAACATTTTGTCGATGGGCAAATTCTGCTCGACTTTAACCGTTGTGGTGCACCGCTAATTGAAGTAGTTACCGCGCCCGTGATTAAGAGTGCTAAGCAGTCTAAGGCATACTTGCAAAAGCTACGTCAAATTTTGATTGTTAACAACATTTCCAACGCTAAGCTCGAAGATGGCTCGATGCGCAGTGACTGTAATGTATCAGTGCGCTTAAAGGGACAAACTGCTTTTGGCACCAAGGTCGAAATTAAGAACATTAACTCACTCAATAATGTGGAAAAGGCAATTGATTTGGAAATAGCACGTCAAGTCAAAGCACTAATTAAAGGCGAACCGGTTCAACAAGTTACCCTAACTTATGACGACAAAACCAATACCAATGTCTTCATGCGTAAAAAGGACAATTCAGTCGATTACCGTTATTTCATTGAACCCAACATTATGAGCAGTAACATTGATGAACTGCTACAAAAACCCAACAAGGCCTTTAACTTAACTGAGTTCTTTACCAAGCTCAAGGAAGCTGGGGTTAATGAGCAGCTAAATCAATTAGTAGTCGATGATCTGACTCTTTTTAATGCTTACACCAAGATTAACAGTGTCATTAACAGTCCCCAAGATACCATTCGCTGGTTGTGCATTGAGTTAATGGGACAACTCAACAAGCTACAAAAACCGCTCAAGGACAAAACGATTGATCACTTAATTATCCTAATCCAAATGGTACAGAAGGGCACCGTGAACCAAAAACAAGCAAAGCAGTTAATTGAGTTAATGTTAGACAATGGGCAAGACCCCCAATCGCTGGCTAAACTACACAACTTAGAACAGATAACTGATGAAAAACAACTAACGCAAATCATCCAACAAATCTTTAAGGAAAACGAGGGTGAAATCCTTAAGAACTTAGATCGAGTTGAACGAATTCAAAAGCTAATTATAGGTCAAGTTATGCAAAGAACCCACAATCGCGCCAATCCCCAGCAAGTTTTTATAATTGTTGAAAAGTTGCTTCATGATTTCAGCGAAAGAGCAAGCTAA", "aa": "MINFEAIIGIEVHVVLNTATKMFSPAPNQAQNATPNQFINAIDLGLPGTMPQVNEQAVQKALILADALNMQRVQPVLVFDRKHYFYQDLPKGFQITQQNFPIAQNGYVEIVENNKAQRIIIERFHLEEDTAKQHFVDGQILLDFNRCGAPLIEVVTAPVIKSAKQSKAYLQKLRQILIVNNISNAKLEDGSMRSDCNVSVRLKGQTAFGTKVEIKNINSLNNVEKAIDLEIARQVKALIKGEPVQQVTLTYDDKTNTNVFMRKKDNSVDYRYFIEPNIMSSNIDELLQKPNKAFNLTEFFTKLKEAGVNEQLNQLVVDDLTLFNAYTKINSVINSPQDTIRWLCIELMGQLNKLQKPLKDKTIDHLIILIQMVQKGTVNQKQAKQLIELMLDNGQDPQSLAKLHNLEQITDEKQLTQIIQQIFKENEGEILKNLDRVERIQKLIIGQVMQRTHNRANPQQVFIIVEKLLHDFSERAS", "aa_hexdigest": "4e03be2185b8f4886ffdaf587fc56391", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000012B157", "ncbi_nrp_id": "WP_014574916.1", "uniref100_id": "UniRef100_P75533", "db_xrefs": [ "SO:0001217", "UniParc:UPI000012B157", "RefSeq:WP_014574916.1", "UniRef:UniRef100_P75533" ] }, "ips": { "uniref100_id": "UniRef100_P75533", "uniref90_id": "UniRef90_P75533" }, "psc": { "uniref90_id": "UniRef90_P75533", "gene": "gatB", "product": "Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB", "ec_ids": [ "6.3.5.-" ], "uniref50_id": "UniRef50_P75533", "cog_id": "COG0064", "cog_category": "J", "kegg_orthology_id": "K02434", "go_ids": [ "GO:0005524", "GO:0006412", "GO:0006424", "GO:0016884", "GO:0030956", "GO:0050566", "GO:0050567", "GO:0070681" ] }, "pscc": { "uniref50_id": "UniRef50_P75533", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75533" ], "product": "Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B" }, "genes": [ "gatB" ], "id": "APKECPOBEN_548", "locus": "GCA002090215_00512" }, { "type": "cds", "sequence": "contig_1", "start": 291640, "stop": 291921, "strand": "+", "frame": 1, "gene": "mngR", "product": "putative HTH-type transcriptional regulator MG101-like protein", "db_xrefs": [ "COG:COG2188", "COG:K", "GO:0003677", "GO:0003700", "SO:0001217", "UniRef:UniRef50_P75532", "UniRef:UniRef90_P75532" ], "nt": "ATGATTTCAGCGAAAGAGCAAGCTAAAAAAGGTCAAATAATTTACCGTTACATTCTCCTTAAAATTCAAAGCTTTAAATGGCCTGCTGGCACCCGCATCTTCTCTGAACGCCAGCTCGAAATTCGTTTCGCTTCATCTAGAAGTCTCATTCGTACTATCCTCAATACCCTCTTAGGTAAAGATCTAATTCGCCACACTAAAGGTAATGCTGGTTACTTTGTCGCTAAGAATGCTGGTTTTAGTTTCTTTCATAAAACCCAGGACAACAAATTGCCCAAGTGA", "aa": "MISAKEQAKKGQIIYRYILLKIQSFKWPAGTRIFSERQLEIRFASSRSLIRTILNTLLGKDLIRHTKGNAGYFVAKNAGFSFFHKTQDNKLPK", "aa_hexdigest": "f903fecc7b0700eb7a22266261b6d67a", "start_type": "ATG", "rbs_motif": null, "genes": [ "mngR" ], "truncated": "3-prime", "pseudogene": { "start": 291640, "stop": 292224, "observations": { "mutation": [ 291919 ], "stop": [ 291919 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75532", "query_cov": 1.0, "subject_cov": 0.4189189189189189, "identity": 1.0, "score": 187.0, "evalue": 2.17e-58, "gene_start": 1, "gene_end": 93, "reference_start": 1, "reference_end": 93, "reference_sequence": "MISAKEQAKKGQIIYRYILLKIQSFKWPAGTRIFSERQLEIRFASSRSLIRTILNTLLGKDLIRHTKGNAGYFVAKNAGFSFFHKTQDNKLPKWAKLSTLMKNHLREVDRDVFSSIDSGVDFGNFKGLEAKLFDENKKNFLNLSFFGKDDVLQIFSEQNLQEQFFKDFAYNGIVVERKSSLICVDDETKNLLIYDLFYDDNNKFIVAMRSEYLNPRIKIINA" }, "paralog": false, "identity": 0.9948453608247423, "score": 381.0, "evalue": 3.29e-137, "description": "Internal stop codon at 291919. Nonsense mutation around 291919.", "psc": { "uniref90_id": "UniRef90_P75532", "gene": "MngR", "product": "Uncharacterized HTH-type transcriptional regulator MG101 homolog", "uniref50_id": "UniRef50_P75532", "cog_id": "COG2188", "cog_category": "K", "go_ids": [ "GO:0003677", "GO:0003700" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75532", "UniRef:UniRef50_P75532", "COG:COG2188", "COG:K", "GO:0003677", "GO:0003700" ] }, "pscc": { "uniref50_id": "UniRef50_P75532", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75532" ], "product": "Uncharacterized HTH-type transcriptional regulator MG101 homolog" } }, "id": "APKECPOBEN_549", "locus": "GCA002090215_00513" }, { "type": "cds", "sequence": "contig_1", "start": 291940, "stop": 292308, "strand": "+", "frame": 1, "gene": "mngR", "product": "putative HTH-type transcriptional regulator MG101-like protein", "db_xrefs": [ "COG:COG2188", "COG:K", "GO:0003677", "GO:0003700", "SO:0001217", "UniRef:UniRef50_P75532", "UniRef:UniRef90_P75532" ], "nt": "ATGAAGAACCACCTACGCGAAGTTGACCGTGATGTTTTCAGCTCAATTGACAGTGGCGTTGACTTTGGTAACTTTAAGGGTTTGGAAGCCAAGTTATTTGATGAAAACAAGAAGAATTTTTTAAACCTTAGCTTCTTTGGCAAGGATGATGTGCTCCAAATTTTTAGCGAGCAAAACCTCCAGGAACAATTCTTTAAGGACTTTGCTTACAACGGCATTGTGGTAGAGCGCAAAAGCTCCTTAATCTGTGTGGATGATGAGACTAAGAACTTACTGATCTACGACCTCTTTTATGATGACAACAATAAGTTCATAGTAGCAATGCGCTCTGAATATTTAAACCCCCGCATCAAAATTATTAATGCTTAA", "aa": "MKNHLREVDRDVFSSIDSGVDFGNFKGLEAKLFDENKKNFLNLSFFGKDDVLQIFSEQNLQEQFFKDFAYNGIVVERKSSLICVDDETKNLLIYDLFYDDNNKFIVAMRSEYLNPRIKIINA", "aa_hexdigest": "4af42ae1fda77222ae6c7c66b9bf9861", "start_type": "ATG", "rbs_motif": null, "genes": [ "mngR" ], "truncated": "5-prime", "pseudogene": { "start": 291640, "stop": 292308, "observations": { "mutation": [ 291919 ], "stop": [ 291919 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75532", "query_cov": 1.0, "subject_cov": 0.5495495495495496, "identity": 1.0, "score": 246.0, "evalue": 2.77e-81, "gene_start": 1, "gene_end": 122, "reference_start": 101, "reference_end": 222, "reference_sequence": "MISAKEQAKKGQIIYRYILLKIQSFKWPAGTRIFSERQLEIRFASSRSLIRTILNTLLGKDLIRHTKGNAGYFVAKNAGFSFFHKTQDNKLPKWAKLSTLMKNHLREVDRDVFSSIDSGVDFGNFKGLEAKLFDENKKNFLNLSFFGKDDVLQIFSEQNLQEQFFKDFAYNGIVVERKSSLICVDDETKNLLIYDLFYDDNNKFIVAMRSEYLNPRIKIINA" }, "paralog": false, "identity": 0.9954954954954955, "score": 437.0, "evalue": 8.44e-159, "description": "Internal stop codon at 291919. Nonsense mutation around 291919.", "psc": { "uniref90_id": "UniRef90_P75532", "gene": "MngR", "product": "Uncharacterized HTH-type transcriptional regulator MG101 homolog", "uniref50_id": "UniRef50_P75532", "cog_id": "COG2188", "cog_category": "K", "go_ids": [ "GO:0003677", "GO:0003700" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75532", "UniRef:UniRef50_P75532", "COG:COG2188", "COG:K", "GO:0003677", "GO:0003700" ] }, "pscc": { "uniref50_id": "UniRef50_P75532", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75532" ], "product": "Uncharacterized HTH-type transcriptional regulator MG101 homolog" } }, "id": "APKECPOBEN_550", "locus": "GCA002090215_00514" }, { "type": "cds", "sequence": "contig_1", "start": 292301, "stop": 293248, "strand": "+", "frame": 2, "gene": "trxB", "product": "thioredoxin-disulfide reductase", "db_xrefs": [ "COG:COG0492", "COG:O", "EC:1.8.1.9", "GO:0004791", "GO:0005737", "GO:0019430", "GO:0071949", "RefSeq:WP_010874597.1", "SO:0001217", "UniParc:UPI00001376C8", "UniRef:UniRef100_P75531", "UniRef:UniRef50_P47348", "UniRef:UniRef90_P75531" ], "nt": "ATGCTTAAAGTAAAGTCAGATTTTTTAACTAAAGATCAGGTTATTTACGATGTAGCGATAGTGGGTGCTGGGCCAGCTGGCATAGCAGCGGGTATTTATGGCAAACGCGCTAACCTCAATCTAGCCATTATTGAAGGTAGTACTCCCGGCGGTAAGGTTGTCAAAACCAACATTGTCGAGAACTATCCGGGTTACAAGTCAATTACCGGACCTGACTTGGGGTTGGAAATGTACAACCACCTAATTGATTTAGAGCCCACCTTTTTCTATGCTAACCTAATTAAGTTAGATAAGGCAGCAGATACCTTTATCTTGTACCTCGATGACAAAACTGTGGTGTTTGCCAAAACCGTTATTTACGCTACGGGAATGTTGGAGCGTAAACTGGGCGTAGCCAAAGAAGACCACTTTTACGGTAAGGGAATTAGTTACTGTGCCATTTGTGATGGGTCCTTGTACAAAGACCAGGTGGTGGGTGTTGTTGGTGGGGGGAACTCTGCCATTCAGGAAGCGTTGTATTTAGCTAGCATGGCTAAAACGGTGCACTTAATCCACCGCCGTGAGGGCTTTCGTGCTGATGAAACGGCGTTAAATAAACTGAGAAACTTACCGAATGTTGTTTTTCACTTGAACTATACCGTAAAAGAATTGTTGGGTAACAATACCCTAAACGGCATAGTGCTTCAAAACACGCTCGATCATTCAACAAAACAGATAGATCTCAATTGTGTCTTTCCCTATATTGGTTTTGAAAGTATTACCAAACCGGTGGAACACCTCAACTTAAAGTTAGATCCCCAGGGTTTTTTAATTACCAATGAACAGATGGAAACAAGTTTAAAGGGTCTGTTTGCAGCAGGTGATTGTCGTTCTAAGCACTTCCGTCAAATTGGTACTGCCATTAATGACGGCATTATCGCGGTGTTAACGATCCGTGATGTCCTTTAG", "aa": "MLKVKSDFLTKDQVIYDVAIVGAGPAGIAAGIYGKRANLNLAIIEGSTPGGKVVKTNIVENYPGYKSITGPDLGLEMYNHLIDLEPTFFYANLIKLDKAADTFILYLDDKTVVFAKTVIYATGMLERKLGVAKEDHFYGKGISYCAICDGSLYKDQVVGVVGGGNSAIQEALYLASMAKTVHLIHRREGFRADETALNKLRNLPNVVFHLNYTVKELLGNNTLNGIVLQNTLDHSTKQIDLNCVFPYIGFESITKPVEHLNLKLDPQGFLITNEQMETSLKGLFAAGDCRSKHFRQIGTAINDGIIAVLTIRDVL", "aa_hexdigest": "68e8c7593f496f8c68f04736c8fdcfe0", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001376C8", "ncbi_nrp_id": "WP_010874597.1", "uniref100_id": "UniRef100_P75531", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001376C8", "RefSeq:WP_010874597.1", "UniRef:UniRef100_P75531" ] }, "ips": { "uniref100_id": "UniRef100_P75531", "uniref90_id": "UniRef90_P75531" }, "psc": { "uniref90_id": "UniRef90_P75531", "gene": "trxB", "product": "thioredoxin-disulfide reductase", "ec_ids": [ "1.8.1.9" ], "uniref50_id": "UniRef50_P47348", "cog_id": "COG0492", "cog_category": "O", "go_ids": [ "GO:0004791", "GO:0005737", "GO:0019430", "GO:0071949" ] }, "pscc": { "uniref50_id": "UniRef50_P47348", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47348" ], "product": "Thioredoxin reductase" }, "genes": [ "trxB" ], "id": "APKECPOBEN_551", "locus": "GCA002090215_00515" }, { "type": "cds", "sequence": "contig_1", "start": 293394, "stop": 294080, "strand": "+", "frame": 3, "gene": "whiA", "product": "putative cell division protein WhiA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47349" ], "nt": "TTGTTTAGCAATGCACTCCAGTTTAGTTTTCAAATCCACGAAACGAGCACTCACTGTGAGTTTAGTTTTAGGGCTTCGCAAACAGAGGTGGATCAGTTGTTAGCCTTTGATGCAACTCAGAGCGATTTACCGCTACAGAAGGCCTATGTCATTGGTGCCTTTTTGAGTGGTGGTAGTGTGAGCGATTTACTCCATTCCAGTAACTTTCACCTCCAAATTAGCACTAACAACGAACTGCAAATAAAACAGTTAATGAAGCTGTTTCATCCTTTTAAACAAACCACTAAGCGCCACCAGCTTTTGGTCTATGTTAAAAGCTATCAGGCCATTTGTGACTTCTTTAAACTAGTCGAAGCTTTTGATGGTTATCTAGCCTTTGAAGAAAACCAATTGCAAAAAAATTTTGCCTTAGAACAAGTGCGTAAAAGTAACTTAGAAATTGCTAACCTAATGCGCACTTTAAAGACCAGCACAAGCACAGCGGAACAGCTAAAAATCCTCATAAACAGTGCTGACTTTAAAAAGCAATCGCTCAACTTTCAGCGCTTTTGTTTAGTCAAACTAGACCATCCTGACTGGTCATTGGAACAAATTGCCCAGTTTTTTGAAAGGAAATACAAAGTGCAAATTACCCGTTCGGGAATTCAACACCTCAACGCTAAGTTAAAAAAACTAAACCAAAATTAA", "aa": "MFSNALQFSFQIHETSTHCEFSFRASQTEVDQLLAFDATQSDLPLQKAYVIGAFLSGGSVSDLLHSSNFHLQISTNNELQIKQLMKLFHPFKQTTKRHQLLVYVKSYQAICDFFKLVEAFDGYLAFEENQLQKNFALEQVRKSNLEIANLMRTLKTSTSTAEQLKILINSADFKKQSLNFQRFCLVKLDHPDWSLEQIAQFFERKYKVQITRSGIQHLNAKLKKLNQN", "aa_hexdigest": "d398808f0140b4e45111776700f91edf", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47349", "query_cov": 0.9868421052631579, "subject_cov": 0.8035714285714286, "identity": 0.569, "score": 258.0, "evalue": 3.35e-83, "valid": false, "gene": "whiA", "product": "DNA-binding protein WhiA", "uniref50_id": "UniRef50_P47349", "cog_id": "COG1481", "cog_category": "K", "go_ids": [ "GO:0003677", "GO:0043937", "GO:0051301" ] }, "pscc": { "uniref50_id": "UniRef50_P47349", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47349" ], "product": "Probable cell division protein WhiA" }, "genes": [ "whiA" ], "id": "APKECPOBEN_552", "locus": "GCA002090215_00516" }, { "type": "cds", "sequence": "contig_1", "start": 294113, "stop": 294343, "strand": "+", "frame": 2, "gene": "secG", "product": "preprotein translocase subunit SecG", "db_xrefs": [ "GO:0005886", "GO:0009306", "GO:0015136", "GO:0015450", "GO:0015739", "GO:0016020", "RefSeq:WP_010874599.1", "SO:0001217", "UniParc:UPI00001357BB", "UniRef:UniRef100_Q9EXD0", "UniRef:UniRef50_P58061", "UniRef:UniRef90_Q9EXD0" ], "nt": "ATGGACGCAATTCAAATAGTAATGTTTGTAATGGCAATCCTTTGTTTAATCATCGGTTTATTGCTATCCAACCATGGTTCTACTGGCGGACTTGCTTCGCTTTCAGGTCAAGATTTGGAAATTTTTCGCAAAACCAAAGACCGCGGAATTGTCAAAATTCTCCAAATTACGATGTTTATTTTAGTAGTGCTTTTTCTTATTTTAGGGTTAGTTTTTCATTTTGCGCTTTAA", "aa": "MDAIQIVMFVMAILCLIIGLLLSNHGSTGGLASLSGQDLEIFRKTKDRGIVKILQITMFILVVLFLILGLVFHFAL", "aa_hexdigest": "45076910ab79b362bfaf44991ea0252c", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001357BB", "ncbi_nrp_id": "WP_010874599.1", "uniref100_id": "UniRef100_Q9EXD0", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001357BB", "RefSeq:WP_010874599.1", "UniRef:UniRef100_Q9EXD0" ] }, "ips": { "uniref100_id": "UniRef100_Q9EXD0", "uniref90_id": "UniRef90_Q9EXD0" }, "psc": { "uniref90_id": "UniRef90_Q9EXD0", "gene": "secG", "product": "preprotein translocase subunit SecG", "uniref50_id": "UniRef50_P58061", "go_ids": [ "GO:0005886", "GO:0009306", "GO:0015136", "GO:0015450", "GO:0015739", "GO:0016020" ] }, "pscc": { "uniref50_id": "UniRef50_P58061", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P58061" ], "product": "Probable protein-export membrane protein SecG" }, "genes": [ "secG" ], "id": "APKECPOBEN_553", "locus": "GCA002090215_00517" }, { "type": "cds", "sequence": "contig_1", "start": 294354, "stop": 294515, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGTATTAACTGATTTACAAAAGCGAATCTTCGCGATTGTGAAAAAAGAGAACGGCAAACCTATTCCTCCGGGAATAGTTGTCAGGATGATGGAGAATCAAGCTGGTTTTCCGGGAAAGCAACAGGTATACAGAGCAATTGATGACTTGTTAGAATGA", "aa": "MKVLTDLQKRIFAIVKKENGKPIPPGIVVRMMENQAGFPGKQQVYRAIDDLLE", "aa_hexdigest": "29b02c844d00215c9ae3be8493903c03", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6025.118200000003, "isoelectric_point": 9.599229621887208 }, "id": "APKECPOBEN_554", "locus": "GCA002090215_00518" }, { "type": "cds", "sequence": "contig_1", "start": 294585, "stop": 295532, "strand": "+", "frame": 3, "gene": "rnr", "product": "ribonuclease R", "db_xrefs": [ "COG:COG0557", "COG:K", "EC:3.1.13.1", "GO:0003723", "GO:0004518", "GO:0005829", "GO:0006402", "GO:0008859", "KEGG:K12573", "SO:0001217", "UniRef:UniRef50_P47350", "UniRef:UniRef90_P75529" ], "nt": "TTGGATCAAAAGTTTCAGGGCATTCTCAATTTAGGTAACAAGAACACTGGTTTTGTCCGTCCCTTAGATGATGACAAAACGGTGTACTATATCCATTTTTCCAACCTAGCGGGAGCACTGGATGGTGATCTAGTTGAATTCTGTCCGCTCGACAAACCACAGGTAGGTGATAAGTTTGATGCTGCAGTGCTCAAAATTGTGAAGCGCTCCCGGGTGCTGTACGCTGGTAACTTTTTGATTGAGTACTCCGATTTTGGTCAGGAGTTTCGCATTGTTGCTGACAATCCTCGTTTTTACCTCACCCCGATTGTCAATAAGGCCTCAGTTCCAGCTGAACTGGAGAGCAATACTAAGGTCGCTTTTCAAATTGATGAGTATGATCCTGCAAATAACCTGTGTAAAGTCTCCATTCAGCAAATCTTGGGGAATAACGATGAACCGTTAATTAACCTCAAGGCGATTATGTTAGACCACTCGATTGTCTTTGAAGATAATGATGTGGTCGAACAACAAGCAGCGAAGTTACAGTTTGATGAAAAAGAGCAGAGTAAGCCCTACCGCAAGGACTTAACCGAACTTGCCTTTGTCACGATTGACCCCGCCACTTCCAAGGACTTGGATGATGCGATTTACGTCAAGCGCACCGACAAAGGTTTTGTTTTGTATGTTGCCATTGCTGATGTGGCTTACTATGTGCAGCGCAACAGTGAACTTGACATTGAAGCCCGTCATAAAACAAGCTCAATTTACCTCCCGGGATACTATGTGGTACCGATGTTACCAGAACGTTTATCAAACGAGCTGTGTTCTTTAAACCCAAATGAAAAGCGGTATGTGGTGGTGTGTGAGTTGAACTTTGATCATGAAGCACGCTTAAACTTTAGTGAAGTGTATCCCGCTACCATTGTCTCCCAACGTCGCTTTGCTTACAGTGAAGTTAATGATTGA", "aa": "MDQKFQGILNLGNKNTGFVRPLDDDKTVYYIHFSNLAGALDGDLVEFCPLDKPQVGDKFDAAVLKIVKRSRVLYAGNFLIEYSDFGQEFRIVADNPRFYLTPIVNKASVPAELESNTKVAFQIDEYDPANNLCKVSIQQILGNNDEPLINLKAIMLDHSIVFEDNDVVEQQAAKLQFDEKEQSKPYRKDLTELAFVTIDPATSKDLDDAIYVKRTDKGFVLYVAIADVAYYVQRNSELDIEARHKTSSIYLPGYYVVPMLPERLSNELCSLNPNEKRYVVVCELNFDHEARLNFSEVYPATIVSQRRFAYSEVND", "aa_hexdigest": "2673ef501b9531c04144ba9e0b1ee5da", "start_type": "TTG", "rbs_motif": null, "genes": [ "rnr" ], "truncated": "both", "pseudogene": { "start": 294354, "stop": 295835, "observations": { "mutation": [ 294513, 295530, 295779 ], "stop": [ 294513, 295530, 295779 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75529", "query_cov": 1.0, "subject_cov": 0.43388429752066116, "identity": 0.997, "score": 624.0, "evalue": 1.43e-219, "gene_start": 1, "gene_end": 315, "reference_start": 78, "reference_end": 392, "reference_sequence": "MKVLTDLQKRIFAIVKKENGKPIPPGIVVRMMENQAGFPGKQQVYRAIDDLLEWHIFRKSGGATNQLLINYELADPVLDQKFQGILNLGNKNTGFVRPLDDDKTVYYIHFSNLAGALDGDLVEFCPLDKPQVGDKFDAAVLKIVKRSRVLYAGNFLIEYSDFGQEFRIVADNPRFYLTPIVNKASVPAELESNTKVAFQIDEYDPANNLCKVSIQQILGNNDEPLINLKAIMLDHSIVFEDNDVVEQQAAKLQFDEKEQSKPYRKDLTELAFVTIDPATSKDLDDAIYVKRTDKGFVLYVAIADVAYYVQRNSELDIEARHKTSSIYLPGYYVVPMLPERLSNELCSLNPNEKRYVVVCELNFDHEARLNFSEVYPATIVSQRRFAYSEVNDWLEDSDALKDESATVLESLKAGFTLSELIAEQRKKKGTIDLSHSETEVVVDQNYYPIEIRFLTHGKAETMIENLMVVANEAVAWTLTNHKVHLPYRVHPRPSKKKLQMLLENIVELKITQPNFVLDTVTSTQIAAWLKENKDNPSYDIFVILLLRTLGKAFYIVNPLIHFSIGSHHYTHFTSPIRRYADLTVHRLLWMNLFTPERFTDTEREQLNAELEQICETINDTEIKINGCERTANDYLTTLYLSKQVGQTFHGFISAITSFGIFMRMDENNFDGLIKITSIPEDFFVFEKDRMVLRGKRTNKVFRIGDRLTAKLTEIDTVQKRAILTLV" }, "paralog": false, "identity": 0.9939148073022313, "score": 967.0, "evalue": 0.0, "description": "Internal stop codon at 294513, 295530, 295779. Nonsense mutation around 294513, 295530, 295779.", "psc": { "uniref90_id": "UniRef90_P75529", "gene": "rnr", "product": "ribonuclease R", "ec_ids": [ "3.1.13.1" ], "uniref50_id": "UniRef50_P47350", "cog_id": "COG0557", "cog_category": "K", "kegg_orthology_id": "K12573", "go_ids": [ "GO:0003723", "GO:0004518", "GO:0005829", "GO:0006402", "GO:0008859" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75529", "EC:3.1.13.1", "UniRef:UniRef50_P47350", "COG:COG0557", "COG:K", "KEGG:K12573", "GO:0003723", "GO:0004518", "GO:0005829", "GO:0006402", "GO:0008859" ] }, "pscc": { "uniref50_id": "UniRef50_P47350", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47350" ], "product": "Ribonuclease R" } }, "id": "APKECPOBEN_555", "locus": "GCA002090215_00519" }, { "type": "cds", "sequence": "contig_1", "start": 295602, "stop": 295781, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00773.24" ], "nt": "TTGAGCGAATTGATTGCTGAACAGCGCAAAAAGAAGGGGACTATTGACCTATCTCACAGTGAAACAGAAGTAGTCGTTGATCAAAACTACTACCCGATTGAGATCCGTTTTCTCACCCACGGCAAGGCGGAAACAATGATCGAAAACCTAATGGTGGTTGCCAATGAAGCGGTGGCTTGA", "aa": "MSELIAEQRKKKGTIDLSHSETEVVVDQNYYPIEIRFLTHGKAETMIENLMVVANEAVA", "aa_hexdigest": "077f519de39ec05950be0135225b340a", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00773.24", "name": "RNB", "length": 59, "aa_cov": 0.9830508474576272, "hmm_cov": 1.0, "evalue": 2.3322835704794533e-10, "score": 35.65190887451172, "start": 2, "stop": 59 } ], "seq_stats": { "molecular_weight": 6691.574899999998, "isoelectric_point": 4.951040077209471 }, "id": "APKECPOBEN_556", "locus": "GCA002090215_00520" }, { "type": "cds", "sequence": "contig_1", "start": 295851, "stop": 296534, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00773.24", "PFAM:PF00575.29" ], "nt": "ATGTTGTTGGAAAACATTGTCGAGCTCAAAATTACCCAACCAAACTTTGTACTCGATACGGTCACTTCCACCCAAATTGCGGCTTGGTTAAAGGAAAATAAGGACAACCCGAGCTATGACATCTTTGTCATCTTGTTATTAAGAACGCTAGGTAAAGCCTTTTACATTGTTAATCCACTGATACACTTTAGCATTGGTTCACACCACTATACCCATTTCACTTCGCCCATCCGTCGCTATGCTGATTTAACGGTACACCGTTTGTTGTGGATGAATCTGTTTACTCCAGAGCGTTTTACTGACACTGAGCGTGAACAGCTCAACGCCGAGCTAGAACAAATTTGTGAAACCATTAACGATACTGAGATTAAGATTAATGGTTGTGAACGGACTGCCAACGATTACTTAACTACGCTGTATTTGTCTAAACAAGTGGGACAAACTTTCCACGGTTTTATTTCCGCTATTACTAGTTTTGGCATCTTTATGCGAATGGATGAAAACAACTTTGATGGACTTATTAAGATTACCTCCATTCCCGAAGACTTCTTTGTCTTTGAAAAGGATCGGATGGTGTTGCGCGGCAAACGCACCAACAAGGTTTTTAGAATTGGTGATAGGTTAACCGCGAAACTGACTGAAATTGATACAGTGCAGAAACGCGCCATTCTAACCCTGGTTTAA", "aa": "MLLENIVELKITQPNFVLDTVTSTQIAAWLKENKDNPSYDIFVILLLRTLGKAFYIVNPLIHFSIGSHHYTHFTSPIRRYADLTVHRLLWMNLFTPERFTDTEREQLNAELEQICETINDTEIKINGCERTANDYLTTLYLSKQVGQTFHGFISAITSFGIFMRMDENNFDGLIKITSIPEDFFVFEKDRMVLRGKRTNKVFRIGDRLTAKLTEIDTVQKRAILTLV", "aa_hexdigest": "bdab34d4f3f66ca21b8330091a27f971", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00773.24", "name": "RNB", "length": 75, "aa_cov": 0.32599118942731276, "hmm_cov": 0.9466666666666667, "evalue": 4.080166919150059e-21, "score": 71.00458526611328, "start": 20, "stop": 93 }, { "id": "PF00575.29", "name": "S1", "length": 82, "aa_cov": 0.36123348017621143, "hmm_cov": 0.8536585365853658, "evalue": 2.107610828558319e-11, "score": 39.560325622558594, "start": 144, "stop": 225 } ], "seq_stats": { "molecular_weight": 26401.13100000002, "isoelectric_point": 6.312959098815918 }, "id": "APKECPOBEN_557", "locus": "GCA002090215_00521" }, { "type": "cds", "sequence": "contig_1", "start": 296534, "stop": 297142, "strand": "+", "frame": 2, "gene": "cdaM", "product": "diadenylate cyclase CdaM", "db_xrefs": [ "COG:COG1624", "COG:T", "EC:2.7.7.85", "GO:0004016", "GO:0005524", "GO:0005886", "GO:0006171", "GO:0106408", "KEGG:K18672", "RefSeq:WP_010874601.1", "SO:0001217", "UniParc:UPI000013928C", "UniRef:UniRef100_P75528", "UniRef:UniRef50_P47351", "UniRef:UniRef90_P75528" ], "nt": "ATGATGACAGTGGAAGTCTTTTCGGTTATTATCCTTGTCCTTTTATTCTTAATCTTAGCGCTTACCTTGTTGTTTGTTTTACTCAACAAGCGTACTAGAAGCTTTGTTATTCGTACCTTTACTGGATTATTTAGATCAAAGCATACTACCAGTCAAAAGAACTTTTATGACAACTTGACCTCAACCTTACTGAGGTTATCAACTGATAAGATTGGTGCCATTATTGCCATTGAAAACCAGGACTCACTCGAGTCTTACGTCAATATTGGCTACCGCGTGACTTCGGACTTTTCCCCCGAGTTGTTAGTAACGATCTTTTACAACAAGCAGTCGCCACTTCACGATGGAGCGGTAATTGTGCGGGACTACCAGATTGTGAGTGTGTCCTCTTACTTCCCAATGACACGGCAACTAATTGATGTGTCATACGGTTCCAGACACCGCAGCGCGTTAGGTCTAACCGAAAAGTGTGATGCCATAGTCTTTATTGTTAGTGAAACAACAGGCAAAATTTCGGTGGCTGTGCGCGGCGTGATTAAAACACTGTCGAGTAACTCCGACCGTTTACAAGACCAGATTATTCACTATCTGACGGTAAAGCCGGGTTAA", "aa": "MMTVEVFSVIILVLLFLILALTLLFVLLNKRTRSFVIRTFTGLFRSKHTTSQKNFYDNLTSTLLRLSTDKIGAIIAIENQDSLESYVNIGYRVTSDFSPELLVTIFYNKQSPLHDGAVIVRDYQIVSVSSYFPMTRQLIDVSYGSRHRSALGLTEKCDAIVFIVSETTGKISVAVRGVIKTLSSNSDRLQDQIIHYLTVKPG", "aa_hexdigest": "bd9fb3567248746504c0b4dd43ffbc94", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013928C", "ncbi_nrp_id": "WP_010874601.1", "uniref100_id": "UniRef100_P75528", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013928C", "RefSeq:WP_010874601.1", "UniRef:UniRef100_P75528" ] }, "ips": { "uniref100_id": "UniRef100_P75528", "uniref90_id": "UniRef90_P75528" }, "psc": { "uniref90_id": "UniRef90_P75528", "gene": "cdaM", "product": "diadenylate cyclase CdaM", "ec_ids": [ "2.7.7.85" ], "uniref50_id": "UniRef50_P47351", "cog_id": "COG1624", "cog_category": "T", "kegg_orthology_id": "K18672", "go_ids": [ "GO:0005886", "GO:0004016", "GO:0005524", "GO:0106408", "GO:0006171" ] }, "pscc": { "uniref50_id": "UniRef50_P47351", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47351" ], "product": "Diadenylate cyclase" }, "genes": [ "cdaM" ], "id": "APKECPOBEN_558", "locus": "GCA002090215_00522" }, { "type": "cds", "sequence": "contig_1", "start": 297288, "stop": 297473, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01327.26" ], "nt": "ATGTTCTACATCCACCTAATGGATGGTGGGGTGGAACACAAGTGTCTGTTAATTAATCCCAAGATTATCAACTTAAGTGCCAACAAATCCTTTTTAAAGAGCGGTGAGGGTTGTTTGAGTGTTCCCAAAATGCACCAGGGTTATGTTATTCGCCACGAATGGATTACGATCACTGGTTTTGATTGA", "aa": "MFYIHLMDGGVEHKCLLINPKIINLSANKSFLKSGEGCLSVPKMHQGYVIRHEWITITGFD", "aa_hexdigest": "159f11430901299627617eef6d196b42", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01327.26", "name": "Pep_deformylase", "length": 63, "aa_cov": 1.0, "hmm_cov": 0.9523809523809523, "evalue": 3.5096439471518963e-13, "score": 44.89892578125, "start": 1, "stop": 61 } ], "seq_stats": { "molecular_weight": 6932.0999, "isoelectric_point": 7.846785545349122 }, "id": "APKECPOBEN_559", "locus": "GCA002090215_00523" }, { "type": "cds", "sequence": "contig_1", "start": 297480, "stop": 297662, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCTGGACGATGTTAAGGAAATTAACGAACCGACTAAACCAGTTCAGTTTCCCTTAGACCAAGCGAGTCTCGATTGCATTGCCAAAATGATGGCTTACGTTGATGCATCTTACAACGGTGATGCTGAGAAGTATGGCATTATTCCCGGCATTGGGATTGCCGCTAACCAAATTGGTTATTGA", "aa": "MLDDVKEINEPTKPVQFPLDQASLDCIAKMMAYVDASYNGDAEKYGIIPGIGIAANQIGY", "aa_hexdigest": "9066c405d33e14da462147f6c20165a3", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6507.353900000002, "isoelectric_point": 4.122554969787596 }, "id": "APKECPOBEN_560", "locus": "GCA002090215_00524" }, { "type": "cds", "sequence": "contig_1", "start": 297733, "stop": 297954, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00005.33", "PFAM:PF00625.27" ], "nt": "ATGGTCGATACAGGGAGAATTTTTGTCATTACCGGACCGAGCGGTGTTGGCAAAAGTAGCCTTGTTAGATGCTTAATTGACCATTTCAAAGATAAACTGCGCTACAGCATTTCCGCTACCACCCGTAAAATGCGTAACAGCGAAACTGAGGGTGTGGATTACTTCTTTAAAGATAAAGCGGAGTTTGAAAAACTGATTGCTGCTGATGCCTTTGTGGAGTGA", "aa": "MVDTGRIFVITGPSGVGKSSLVRCLIDHFKDKLRYSISATTRKMRNSETEGVDYFFKDKAEFEKLIAADAFVE", "aa_hexdigest": "bb55ab2126a223b29d544b78bb1acdbb", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00005.33", "name": "ABC_tran", "length": 67, "aa_cov": 0.8767123287671232, "hmm_cov": 1.0, "evalue": 1.0391755207007253e-07, "score": 28.087451934814453, "start": 2, "stop": 65 }, { "id": "PF00625.27", "name": "Guanylate_kin", "length": 68, "aa_cov": 0.9315068493150684, "hmm_cov": 1.0, "evalue": 2.8299493487290736e-19, "score": 64.94884490966797, "start": 6, "stop": 73 } ], "seq_stats": { "molecular_weight": 8237.359199999997, "isoelectric_point": 7.869220542907715 }, "id": "APKECPOBEN_561", "locus": "GCA002090215_00525" }, { "type": "cds", "sequence": "contig_1", "start": 297958, "stop": 298302, "strand": "+", "frame": 1, "gene": "gmk", "product": "guanylate kinase", "db_xrefs": [ "COG:COG0194", "COG:F", "EC:2.7.4.8", "GO:0004385", "GO:0005524", "GO:0005829", "GO:0006163", "SO:0001217", "UniRef:UniRef50_P47353", "UniRef:UniRef90_P75526" ], "nt": "ATGTATAACGATAACTACTATGGTACCCTTAAATCCCAAGCTGAACAAATTATTCACAACGGTGGCAACTTGGTGTTAGAAATTGAATATCAAGGTGCTTTACAAGTTAAGCAAAAGTATCCCAATGATGTGGTGCTAATTTTTATTAAACCACCTTCAATGGAAGAGTTGTTAGTGCGCTTAAAGAAGCGTAATGACGAGGATGCAATAACAATTCAAAACCGATTGAAACAAGCTGAGAAGGAATGTCAACAAATTGGTCACTTTAAGTATGTGGTTACCAACAACGAGTTTGACAAGACCTTAGCGGAGTTACAAGCAATTTTACTAGCTGAATTTAATTAA", "aa": "MYNDNYYGTLKSQAEQIIHNGGNLVLEIEYQGALQVKQKYPNDVVLIFIKPPSMEELLVRLKKRNDEDAITIQNRLKQAEKECQQIGHFKYVVTNNEFDKTLAELQAILLAEFN", "aa_hexdigest": "b9b1766fd16e18151b3704ab3886d132", "start_type": "ATG", "rbs_motif": null, "genes": [ "gmk" ], "truncated": "5-prime", "pseudogene": { "start": 297658, "stop": 298302, "observations": { "mutation": [ 297952 ], "stop": [ 297952 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75526", "query_cov": 1.0, "subject_cov": 0.4769874476987448, "identity": 1.0, "score": 224.0, "evalue": 1.62e-72, "gene_start": 1, "gene_end": 114, "reference_start": 126, "reference_end": 239, "reference_sequence": "MQSRLAWSKGNWTGLVGSLISLTSSSTSHALVGNNSILICGKIKIILNIEMVDTGRIFVITGPSGVGKSSLVRCLIDHFKDKLRYSISATTRKMRNSETEGVDYFFKDKAEFEKLIAADAFVEWAMYNDNYYGTLKSQAEQIIHNGGNLVLEIEYQGALQVKQKYPNDVVLIFIKPPSMEELLVRLKKRNDEDAITIQNRLKQAEKECQQIGHFKYVVTNNEFDKTLAELQAILLAEFN" }, "paralog": false, "identity": 0.9953271028037384, "score": 419.0, "evalue": 1.15e-151, "description": "Internal stop codon at 297952. Nonsense mutation around 297952.", "psc": { "uniref90_id": "UniRef90_P75526", "gene": "gmk", "product": "guanylate kinase", "ec_ids": [ "2.7.4.8" ], "uniref50_id": "UniRef50_P47353", "cog_id": "COG0194", "cog_category": "F", "go_ids": [ "GO:0004385", "GO:0005524", "GO:0005829", "GO:0006163" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75526", "EC:2.7.4.8", "UniRef:UniRef50_P47353", "COG:COG0194", "COG:F", "GO:0004385", "GO:0005524", "GO:0005829", "GO:0006163" ] }, "pscc": { "uniref50_id": "UniRef50_P47353", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47353" ], "product": "Guanylate kinase" } }, "id": "APKECPOBEN_562", "locus": "GCA002090215_00526" }, { "type": "cds", "sequence": "contig_1", "start": 298302, "stop": 298676, "strand": "+", "frame": 3, "gene": "pTC1", "product": "Putative protein phosphatase", "db_xrefs": [ "COG:COG0631", "COG:T", "EC:3.1.3.16", "GO:0017018", "GO:0033192", "GO:0140791", "GO:0180004", "GO:0180005", "GO:0180006", "GO:0180007", "GO:0180008", "GO:1990439", "KEGG:K20074", "SO:0001217", "UniRef:UniRef50_P47354", "UniRef:UniRef90_P75525" ], "nt": "ATGGACAGCACCAACCAAAACCTGTTCGCGTCATTGTCCAAAAAAGGACCCGTTCGCAAAGAAAATCAGGACTTTAGTGTTGTTACTTTCAACCGCTTTGGTCAGTTAATGAGCCTTGTTTGTGATGGTTTAGGCGGTTATAAGGGCGGTAAGATGGCTAGTGCCTTGGTGAGTGAAGTTTTTACCAAGTCCTTTACAGTCTTTGATTTTCATAGTCAAACTGAAAGGGCAGTGAAACAGTGGTTTGAAATTACTTTAATTGAAGCGCGGCGCACCTTAGAACAGTACTTTCAAACAATTAAACGTAACCAAGTCCAGTTTGCGCGAATGGCGACTACCTTAGTGTTGAGTATTATAAGTAAGCAAAACATTTGA", "aa": "MDSTNQNLFASLSKKGPVRKENQDFSVVTFNRFGQLMSLVCDGLGGYKGGKMASALVSEVFTKSFTVFDFHSQTERAVKQWFEITLIEARRTLEQYFQTIKRNQVQFARMATTLVLSIISKQNI", "aa_hexdigest": "a88e295f92a3aa202ea4fc0865483625", "start_type": "ATG", "rbs_motif": null, "genes": [ "pTC1" ], "truncated": "3-prime", "pseudogene": { "start": 298302, "stop": 298979, "observations": { "mutation": [ 298674 ], "stop": [ 298674 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75525", "query_cov": 1.0, "subject_cov": 0.47876447876447875, "identity": 1.0, "score": 242.0, "evalue": 4.74e-79, "gene_start": 1, "gene_end": 124, "reference_start": 1, "reference_end": 124, "reference_sequence": "MDSTNQNLFASLSKKGPVRKENQDFSVVTFNRFGQLMSLVCDGLGGYKGGKMASALVSEVFTKSFTVFDFHSQTERAVKQWFEITLIEARRTLEQYFQTIKRNQVQFARMATTLVLSIISKQNIWTFWVGDSRAYLINSYQSLQITEDHNLYNQLLQMHATPDVIASYKDKLLALTATVSKDQERQLKYSFRCDVVNAWDFLLLCSDGLYNFLDPNCFYEVITSAPNLKKAVTQLAKLSLDNASNDNITLNLINLKQWH" }, "paralog": false, "identity": 0.9955555555555555, "score": 447.0, "evalue": 2.69e-162, "description": "Internal stop codon at 298674. Nonsense mutation around 298674.", "psc": { "uniref90_id": "UniRef90_P75525", "gene": "PTC1", "product": "Putative protein phosphatase", "ec_ids": [ "3.1.3.16" ], "uniref50_id": "UniRef50_P47354", "cog_id": "COG0631", "cog_category": "T", "kegg_orthology_id": "K20074", "go_ids": [ "GO:0033192", "GO:0140791", "GO:1990439", "GO:0017018", "GO:0180006", "GO:0180007", "GO:0180008", "GO:0180005", "GO:0180004" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75525", "EC:3.1.3.16", "UniRef:UniRef50_P47354", "COG:COG0631", "COG:T", "KEGG:K20074", "GO:0033192", "GO:0140791", "GO:1990439", "GO:0017018", "GO:0180006", "GO:0180007", "GO:0180008", "GO:0180005", "GO:0180004" ] }, "pscc": { "uniref50_id": "UniRef50_P47354", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47354" ], "product": "Putative protein phosphatase" } }, "id": "APKECPOBEN_563", "locus": "GCA002090215_00527" }, { "type": "cds", "sequence": "contig_1", "start": 298686, "stop": 299081, "strand": "+", "frame": 3, "gene": "pTC1", "product": "Putative protein phosphatase", "db_xrefs": [ "COG:COG0631", "COG:T", "EC:3.1.3.16", "GO:0017018", "GO:0033192", "GO:0140791", "GO:0180004", "GO:0180005", "GO:0180006", "GO:0180007", "GO:0180008", "GO:1990439", "KEGG:K20074", "SO:0001217", "UniRef:UniRef50_P47354", "UniRef:UniRef90_P75525" ], "nt": "GTGGGTGATTCACGTGCTTATTTAATTAACAGTTACCAAAGCTTGCAAATTACCGAAGACCATAACCTGTACAACCAACTGTTACAGATGCACGCTACCCCTGATGTGATTGCCTCTTATAAAGACAAGCTGTTAGCCCTCACTGCTACCGTTTCCAAAGATCAGGAACGCCAGTTGAAATACAGCTTTAGGTGTGATGTAGTAAACGCTTGGGACTTTTTACTGTTGTGCTCTGATGGTCTGTACAACTTCTTGGATCCCAACTGCTTTTATGAGGTCATTACTAGTGCACCTAACCTCAAAAAGGCCGTTACACAACTAGCAAAGTTGAGCTTGGATAACGCTAGTAATGACAACATTACGCTCAACTTGATTAATTTAAAACAATGGCACTAA", "aa": "MGDSRAYLINSYQSLQITEDHNLYNQLLQMHATPDVIASYKDKLLALTATVSKDQERQLKYSFRCDVVNAWDFLLLCSDGLYNFLDPNCFYEVITSAPNLKKAVTQLAKLSLDNASNDNITLNLINLKQWH", "aa_hexdigest": "cdfd83a2d804c7344bee24f8c1941817", "start_type": "GTG", "rbs_motif": null, "genes": [ "pTC1" ], "truncated": "5-prime", "pseudogene": { "start": 298386, "stop": 299081, "observations": { "mutation": [ 298674 ], "stop": [ 298674 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75525", "query_cov": 1.0, "subject_cov": 0.5057915057915058, "identity": 0.992, "score": 265.0, "evalue": 4.78e-88, "gene_start": 1, "gene_end": 131, "reference_start": 129, "reference_end": 259, "reference_sequence": "MDSTNQNLFASLSKKGPVRKENQDFSVVTFNRFGQLMSLVCDGLGGYKGGKMASALVSEVFTKSFTVFDFHSQTERAVKQWFEITLIEARRTLEQYFQTIKRNQVQFARMATTLVLSIISKQNIWTFWVGDSRAYLINSYQSLQITEDHNLYNQLLQMHATPDVIASYKDKLLALTATVSKDQERQLKYSFRCDVVNAWDFLLLCSDGLYNFLDPNCFYEVITSAPNLKKAVTQLAKLSLDNASNDNITLNLINLKQWH" }, "paralog": false, "identity": 0.9956709956709957, "score": 460.0, "evalue": 2.35e-167, "description": "Internal stop codon at 298674. Nonsense mutation around 298674.", "psc": { "uniref90_id": "UniRef90_P75525", "gene": "PTC1", "product": "Putative protein phosphatase", "ec_ids": [ "3.1.3.16" ], "uniref50_id": "UniRef50_P47354", "cog_id": "COG0631", "cog_category": "T", "kegg_orthology_id": "K20074", "go_ids": [ "GO:0033192", "GO:0140791", "GO:1990439", "GO:0017018", "GO:0180006", "GO:0180007", "GO:0180008", "GO:0180005", "GO:0180004" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75525", "EC:3.1.3.16", "UniRef:UniRef50_P47354", "COG:COG0631", "COG:T", "KEGG:K20074", "GO:0033192", "GO:0140791", "GO:1990439", "GO:0017018", "GO:0180006", "GO:0180007", "GO:0180008", "GO:0180005", "GO:0180004" ] }, "pscc": { "uniref50_id": "UniRef50_P47354", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47354" ], "product": "Putative protein phosphatase" } }, "id": "APKECPOBEN_564", "locus": "GCA002090215_00528" }, { "type": "cds", "sequence": "contig_1", "start": 299072, "stop": 300040, "strand": "+", "frame": 2, "gene": "sPS1", "product": "Putative serine/threonine-protein kinase", "db_xrefs": [ "COG:COG0515", "COG:T", "EC:2.7.11.1", "GO:0004676", "GO:0004677", "GO:0004679", "GO:0004711", "GO:0005524", "GO:0035175", "GO:0035402", "GO:0035403", "GO:0035979", "GO:0044022", "GO:0044023", "GO:0044024", "GO:0044025", "GO:0072354", "GO:0072371", "GO:0072518", "GO:0106310", "GO:0140823", "GO:0140855", "GO:0140857", "GO:1990244", "SO:0001217", "UniRef:UniRef50_P47355", "UniRef:UniRef90_P75524" ], "nt": "ATGGCACTAAATTTAAAGATTGGTGACATTGTCCAAAACAAGTACCGGATTGAAAAGCTAATCAACCGTGGCGGGATGAACTCTTACCTGTTTTTAGCGAGCAACCTCCACGTCCAGGAATTTGGTCCACTGCAAAAGCGCCAGTTTACCCGGTTGGTGTTAAAGGTAGTACAACGCACCGACAAGATTAACGACAACAACTGGAAGAAGTTTTTGGATGGAACCATTACCACGACACGGGTGTCACACAAAAATCTGGTGCAAACCTTTGATGTAGTTAGCCCCCGTTTAAGCGTTTTGAGTGAAAACCAGGTGATCGTTTTAGAGGACACGGTGATGATTGTAATGGAGTATGTTGATGGTCCTTCTTTGCGTGAAATGCTCAACCAGAAGGGTTACTTTAGTGTGCAGGAGGTGGTGTACTACTTTACAAAGCTAGTCAAAGTAATTAACTACCTCCACAGTTTTGAACACCAAATTATTCACCGTGATCTAAAGCCAGAAAACATCCTGTTTACGAGTAACTTGACTGACATTAAACTGCTTGACTTTGGCATTGCTTCCGCTGTTATTCGTAACGCTGAGAAAACTGAAGTGTTAACGGACGAAAACTCGTTGTTTGGTACAGTTAGTTATATGACACCCGAAGTGTTGGAAAGTACTGTCAACAAAGAAGGCAAACGGATTCGTAAACCCCCTACAGTGCAGTATGACATCTATTCATTAGGAGTTATTTTGTTTGAAATGTTAGTCGGACGCGTACCGTTCAATAAATCGATTGATCCTAAAAAAGAACGGGAAACCATCCAAAAGGCGCGTAACTTTGATGTACCTTTAATGGGAAACTTGCGCAGTGATGTTCCGGTTAGTTTGGAAAACATTGTCTTTAAATGTACGGCTGTTAAACGTGAAAACAGTAAGTGGATGTATAGTGACACGAAGCAACTCTTGGCTGATTTGGCGCAATGA", "aa": "MALNLKIGDIVQNKYRIEKLINRGGMNSYLFLASNLHVQEFGPLQKRQFTRLVLKVVQRTDKINDNNWKKFLDGTITTTRVSHKNLVQTFDVVSPRLSVLSENQVIVLEDTVMIVMEYVDGPSLREMLNQKGYFSVQEVVYYFTKLVKVINYLHSFEHQIIHRDLKPENILFTSNLTDIKLLDFGIASAVIRNAEKTEVLTDENSLFGTVSYMTPEVLESTVNKEGKRIRKPPTVQYDIYSLGVILFEMLVGRVPFNKSIDPKKERETIQKARNFDVPLMGNLRSDVPVSLENIVFKCTAVKRENSKWMYSDTKQLLADLAQ", "aa_hexdigest": "3cb71ac14745abba6016be31ecb741d9", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75524", "query_cov": 1.0, "subject_cov": 0.8277634961439588, "identity": 1.0, "score": 629.0, "evalue": 1.02e-226, "valid": true, "gene": "SPS1", "product": "Putative serine/threonine-protein kinase", "ec_ids": [ "2.7.11.1" ], "uniref50_id": "UniRef50_P47355", "cog_id": "COG0515", "cog_category": "T", "go_ids": [ "GO:0004676", "GO:0004679", "GO:0005524", "GO:0004677", "GO:0044024", "GO:0072371", "GO:1990244", "GO:0035979", "GO:0044025", "GO:0140823", "GO:0035175", "GO:0044022", "GO:0140855", "GO:0035402", "GO:0072354", "GO:0140857", "GO:0035403", "GO:0044023", "GO:0106310", "GO:0072518", "GO:0004711" ] }, "pscc": { "uniref50_id": "UniRef50_P47355", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47355" ], "product": "Putative serine/threonine-protein kinase" }, "genes": [ "sPS1" ], "id": "APKECPOBEN_565", "locus": "GCA002090215_00529" }, { "type": "cds", "sequence": "contig_1", "start": 300068, "stop": 300241, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCACGAACGTATTCTAGAAGGACAAAACGAAATGCGTGAACTAATGGTAAGTAATTACCTACCGTGGTATTTAAGGAAGGGTGTTTTGATCTTTTTTTCTGTTGTTTTATTAGCACTCTTAATTGCTGTAGTTAGCTTTTTTATTATTACTGGGGTAGTTGTCCATTCGTAA", "aa": "MHERILEGQNEMRELMVSNYLPWYLRKGVLIFFSVVLLALLIAVVSFFIITGVVVHS", "aa_hexdigest": "1b2f91b1dccc1b0c699623e34bd32845", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6592.876599999998, "isoelectric_point": 6.70656909942627 }, "id": "APKECPOBEN_566", "locus": "GCA002090215_00530" }, { "type": "cds", "sequence": "contig_1", "start": 300501, "stop": 301031, "strand": "+", "frame": 3, "gene": "rsgA", "product": "ribosome small subunit-dependent GTPase A", "db_xrefs": [ "COG:COG1162", "COG:J", "EC:3.6.1.-", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0006412", "GO:0019843", "GO:0042274", "GO:0043022", "GO:0046872", "KEGG:K06949", "SO:0001217", "UniRef:UniRef50_P75523", "UniRef:UniRef90_P75523" ], "nt": "TTGTTTAAATTGTTAATCCATTTTCACGCTCAAAACGTGGCACGGTTAGTGTTAGTCATTACTAAAAACGACCTTGAGTTTGAACCTGCTGAAAAAGCCCGTTTAAGTGAACTAACCAGCTTTGGTTACCAACTCTTTTTTACAGCCCCTAATGCTGATTTACCTCTCACTTTATTTACAGAATTGCAAAACCGTTTTAGTGTATTCATGGGACAATCAGGGGTCGGTAAGTCTAGCCTTATTAACCGTTTGGACAGTCAAATTCACCAAGCGATCCAAGCGCTTTCAGCACACCAGTTCGGTAAAAACACCACCACTTCCACGGTGATGTTTCCCTTCCAAAACGGCTTTATTTGTGACACCCCTGGCTTTAATGTAATTGACTTTCCCAACCTCAAACAATTAGCAGCCCAACACTTTGTCGGTTTTGCTAGCTTAATTGGTCAATGCCATTTTTCCAACTGCACCCATCAAAGCGAAAAAGGTTGTTTTATTGTAAGTGCTGTAACCCAAAAATCGTATTCCTTGTGA", "aa": "MFKLLIHFHAQNVARLVLVITKNDLEFEPAEKARLSELTSFGYQLFFTAPNADLPLTLFTELQNRFSVFMGQSGVGKSSLINRLDSQIHQAIQALSAHQFGKNTTTSTVMFPFQNGFICDTPGFNVIDFPNLKQLAAQHFVGFASLIGQCHFSNCTHQSEKGCFIVSAVTQKSYSL", "aa_hexdigest": "8fb81df9a2babc17ea5d87eaf5e50f5f", "start_type": "TTG", "rbs_motif": null, "genes": [ "rsgA" ], "truncated": "both", "pseudogene": { "start": 300228, "stop": 301064, "observations": { "mutation": [ 300333, 300492, 301029 ], "stop": [ 300333, 300492, 301029 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75523", "query_cov": 1.0, "subject_cov": 0.6330935251798561, "identity": 0.9940000000000001, "score": 352.0, "evalue": 2.33e-121, "gene_start": 1, "gene_end": 176, "reference_start": 92, "reference_end": 267, "reference_sequence": "MSIRKTGIVFQRFGKQFKVFVENQTLVAFAQKKLQWKRDFKLLVGDKVTLENGVIVAVEERKNTLVRPKVVNVDQVVIVQSLIEPKINWQQLFKLLIHFHAQNVARLVLVITKNDLEFEPAEKARLSELTSFGYQLFFTAPNADLPLTLFTELQNRFSVFMGQSGVGKSSLINRLDSQIHQAIQALSAHQFGKNTTTSTVMFPFQNGFICDTPGFNVIDFPNLKQLAAQHFVGFASLIGQCHFSNCTHQSEKGCFIVSAVTQKSYSLWLYESYLKLIN" }, "paralog": false, "identity": 0.9856115107913669, "score": 535.0, "evalue": 7.76e-196, "description": "Internal stop codon at 300333, 300492, 301029. Nonsense mutation around 300333, 300492, 301029.", "psc": { "uniref90_id": "UniRef90_P75523", "gene": "rsgA", "product": "ribosome small subunit-dependent GTPase A", "ec_ids": [ "3.6.1.-" ], "uniref50_id": "UniRef50_P75523", "cog_id": "COG1162", "cog_category": "J", "kegg_orthology_id": "K06949", "go_ids": [ "GO:0003924", "GO:0005525", "GO:0005737", "GO:0006412", "GO:0019843", "GO:0042274", "GO:0043022", "GO:0046872" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75523", "EC:3.6.1.-", "UniRef:UniRef50_P75523", "COG:COG1162", "COG:J", "KEGG:K06949", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0006412", "GO:0019843", "GO:0042274", "GO:0043022", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_P75523", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75523" ], "product": "Small ribosomal subunit biogenesis GTPase RsgA" } }, "id": "APKECPOBEN_567", "locus": "GCA002090215_00531" }, { "type": "cds", "sequence": "contig_1", "start": 301086, "stop": 301478, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00342.24" ], "nt": "ATGGAAAGTAAATGGTTAACAGTTGACACTAAGCACCTCTATGGTTTTGATGAAGCGATTTTTATTCAGAAATACCAAAAAAAAGTTAACCAAATTCACCAACAGTTTTTAAATCAACAACTACCCGATGGTCACATGAATGGCTGGTATAGTCAACCCGATCAAGACCACAAGGGTTTGCTAAAACAAATTAACACAATTGCCAAACAGTTTAATGCTCTAAAAGTAACTGACATAGTGTATTTAGGTATTGGTGGTTCTTATACTGGCATTCGTGCCATCTTAGACTTTTTAAAACCAGAACAGAAGGCGAATATCAAAGTGCACTTTGTTCCTGACATATCGGCCTTTAACATTGCTGCAGTTGCTAGAGCAATTAAGGGTAAATCATGA", "aa": "MESKWLTVDTKHLYGFDEAIFIQKYQKKVNQIHQQFLNQQLPDGHMNGWYSQPDQDHKGLLKQINTIAKQFNALKVTDIVYLGIGGSYTGIRAILDFLKPEQKANIKVHFVPDISAFNIAAVARAIKGKS", "aa_hexdigest": "3ff64ab4288b6a7684f0e40d1caa71db", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00342.24", "name": "PGI", "length": 54, "aa_cov": 0.4153846153846154, "hmm_cov": 0.9814814814814815, "evalue": 1.2307613096534597e-06, "score": 22.81281089782715, "start": 73, "stop": 126 } ], "seq_stats": { "molecular_weight": 14785.84889999998, "isoelectric_point": 9.426003074645994 }, "id": "APKECPOBEN_568", "locus": "GCA002090215_00532" }, { "type": "cds", "sequence": "contig_1", "start": 301482, "stop": 301841, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTAGTAACTTCTAAATCTGGTCGCACACTCGAGCCGGCTGTTACCTTTCGGTACTTCCGTAATTTATTGCACAAACAATACAAGCAAAAACATGCTTTACGCACAGTTGTGATTACCGATGCCGTTAAGGGTTTACTAGTTGGCATGAGTAACCAGTACGGTTATGCACACTTAACTATTCCAAGCAATATTGGTGGGCGTTTTTCAACTCTTTCACCCGCCGGTTTACTGTTAGCTAAACTTTGTGGTCATGATCCGAAACAGCTCTTATTGGGTACTTTGACAGCCAAACAAGAGTTAGCAAACAGTGATTTAAACACTAATTCGGCTTATTATTATGCTGCTTTGCGTCACTGA", "aa": "MVVTSKSGRTLEPAVTFRYFRNLLHKQYKQKHALRTVVITDAVKGLLVGMSNQYGYAHLTIPSNIGGRFSTLSPAGLLLAKLCGHDPKQLLLGTLTAKQELANSDLNTNSAYYYAALRH", "aa_hexdigest": "41cde99de6a42c799c7e91b033a01f86", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13119.045899999983, "isoelectric_point": 9.947874641418458 }, "id": "APKECPOBEN_569", "locus": "GCA002090215_00533" }, { "type": "cds", "sequence": "contig_1", "start": 301872, "stop": 302378, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGACGGTAGCATACCACAGTGCTTATGAGTACCTCTTGTTGCAACACCGCCAACTGTTCGGTGAATCAGAGGGTAAAGGTGGTAAGTCCCTGTTCCCTACCTTTTCGCTATTTACCACTGACTTACACTCAATGGGGCAGTTGTACCAAGAAGGGGAAAAGAACTTCTTTGAAACAGTGATACAAGTGCAAACACAATTTCACGACTTGGAACTACCACCATCAGACTTTAACAATGATGATCAGCTAGATTACTTATTGGCTAAAAGTATGAATGAAATTTCAAACACTGCCCTAGAAGCAGTTGTGGAAGCTCATTTTCAATCAAACGTTAACATCATTAAGTTAACGCTCAAAGAAAGAACAACCTTTATGTTTGGTTACTTTTACTTCTGGTTATCAATGGCCACAATGATGAGTGGTTCACTGCTGGGACACAACGTCTTTGATCAACCAGGGGTGGAAGTGTACAAGCAGTTAATGTTTGCTAAACTTGGTCGTGAATAG", "aa": "MTVAYHSAYEYLLLQHRQLFGESEGKGGKSLFPTFSLFTTDLHSMGQLYQEGEKNFFETVIQVQTQFHDLELPPSDFNNDDQLDYLLAKSMNEISNTALEAVVEAHFQSNVNIIKLTLKERTTFMFGYFYFWLSMATMMSGSLLGHNVFDQPGVEVYKQLMFAKLGRE", "aa_hexdigest": "966487e1881d2146614e255c06480633", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19352.730699999975, "isoelectric_point": 5.042266654968262 }, "id": "APKECPOBEN_570", "locus": "GCA002090215_00534" }, { "type": "cds", "sequence": "contig_1", "start": 302371, "stop": 303003, "strand": "+", "frame": 1, "gene": "rpe", "product": "Ribulose-phosphate 3-epimerase", "db_xrefs": [ "COG:COG0036", "COG:G", "EC:5.1.3.1", "GO:0004750", "GO:0006098", "GO:0019323", "GO:0046872", "SO:0001217", "UniParc:UPI0001DD64FF", "UniRef:UniRef100_A0AB38W7C5", "UniRef:UniRef50_P47358", "UniRef:UniRef90_P75522" ], "nt": "GTGAATAGGGAAATTGCCTTTTCGCTTTTACCACTGTTACACCAGTTTGACCGCAAGTTGTTAGAACAATTCTTTGCGGACGGCTTGCGCTTAATCCACTATGACGTGATGGACCATTTTGTGGACAACACTGTCTTTCAAGGGGAACATTTAGATGAATTACAACAAATTGGTTTTCAGGTCAATGTACATTTGATGGTACAAGCATTAGAACAAATCTTGCCAGTATACCTCCACCATCAAGCCGTTAAGCGCATTTCCTTTCACGTTGAACCGTTTGATATCCCTACCATCAAACACTTTATTGCGCAAATTAAACAAGCGGGAAAACAAGTCGGTTTAGCGTTTAAATTCACCACTCCGCTTGTAAATTACGAGCGCTTGGTGCAACAACTGGACTTTGTTACCTTAATGAGTGTCCCCCCAGGAAAGGGCGGGCAAGCCTTTAACAGTGCTGTTTTTAATAACTTAAAACAAGCCCACAAATACCACTGCTCAATTGAAATCGATGGTGGGATCAAATTAGATAACATTCACCAAATCCAGGATGATGTCAATTTTATTGTCATGGGAAGTGGCTTTATAAAATTAGAACGATGGCAGCGCCAACAGCTGCTCAAAACCAATCAATAA", "aa": "MNREIAFSLLPLLHQFDRKLLEQFFADGLRLIHYDVMDHFVDNTVFQGEHLDELQQIGFQVNVHLMVQALEQILPVYLHHQAVKRISFHVEPFDIPTIKHFIAQIKQAGKQVGLAFKFTTPLVNYERLVQQLDFVTLMSVPPGKGGQAFNSAVFNNLKQAHKYHCSIEIDGGIKLDNIHQIQDDVNFIVMGSGFIKLERWQRQQLLKTNQ", "aa_hexdigest": "179887d09df0e062c70b45ea8ae49ba4", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD64FF", "uniref100_id": "UniRef100_A0AB38W7C5", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD64FF", "UniRef:UniRef100_A0AB38W7C5" ] }, "ips": { "uniref100_id": "UniRef100_A0AB38W7C5", "uniref90_id": "UniRef90_P75522" }, "psc": { "uniref90_id": "UniRef90_P75522", "gene": "rpe", "product": "Ribulose-phosphate 3-epimerase", "ec_ids": [ "5.1.3.1" ], "uniref50_id": "UniRef50_P47358", "cog_id": "COG0036", "cog_category": "G", "go_ids": [ "GO:0004750", "GO:0046872", "GO:0019323", "GO:0006098" ] }, "pscc": { "uniref50_id": "UniRef50_P47358", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47358" ], "product": "Ribulose-phosphate 3-epimerase" }, "genes": [ "rpe" ], "id": "APKECPOBEN_571", "locus": "GCA002090215_00535" }, { "type": "cds", "sequence": "contig_1", "start": 303088, "stop": 304377, "strand": "+", "frame": 1, "gene": "asnS", "product": "asparagine--tRNA ligase", "db_xrefs": [ "COG:COG0017", "COG:J", "EC:6.1.1.22", "GO:0000166", "GO:0003676", "GO:0004816", "GO:0005524", "GO:0005737", "GO:0006421", "KEGG:K01893", "SO:0001217", "UniRef:UniRef50_P47359", "UniRef:UniRef90_P75521" ], "nt": "TTGAAAAACAAACGTACTAGCGCCAACATTATCTTTTTGGAAGTTAATGATGGATCAACGCTGTTAAACCTACAAGCGGTGGTTAAACAAGACCAACCGGAGTTGTTTGCATTAGCTGAATCAATTAGTTTAGCGAGTGCTGTAAGTGTTTCTGGTACTGTAGCGTTAACACCCAAATCCAAACAACCCTTGGAATTAGTAGTCAAACAAATTAATGTATTGAGTACTGCGCGAGCAGACTATCCACTCCAAAAAAAGGAACACAGCTTGGAGTTTTTCCGCAACAACGCCTATTTGCGCGTACGAGCCCGTACCTACTTTGCCATCATGAAAGTGCGCAGTCTGTTGTCACAAGCCATTTTTGACTACTTCTTTAAGAATGACTTTGTTTTAGTGCACAGTCCAATTCTTACTAGCAACGACTGTGAGGGGGCAGGTGAGACCTTTGAATTAAAGCAAGGTAAGGAATTCTTTAATAAGACTACTTACCTTACTGTCAGTGGTCAGTTTGGTGCGGAGTGTTACGCCCAAGCCTTTAAAAAAGTGTTTACCTTCGGGCCGACTTTTCGCGCTGAAAAATCACACACTTCACGGCACCTTAGTGAGTTTTGGATGATTGAACCAGAAGTTGCTTTTGCCAACCTCAAGGACTTAATTAAGCTAATTGAAAGCACTGTCAAAACCGTGATTAAACAGGTTATGCAAAAAGCCAAGCAGGAACTCGACTTTTTAGAAAAGCAGTTTGATGTCAAATTAATGGAACGCTTAAAGCAAATTACAAGTACCAAAAACTTCCATGTTTTGGAATACACCAAGGCCTTAGAGATTTTAAAAACAGCCCAAGCTAGTGGTCAAGCTAACTTTGAGGTACAAGACTTTAACTTCGGTTTAGATCTCAAAACGGAACACGAACGCTTTTTGTGTGAACAGCATTTTCACAACCAACCGGTGTTTGTAATTAACTACCCCAAGGACTTTAAGGCCTTTTACATGAAGCAAAATGCTGATGGTAGAACTGTTGGTGCGGTTGACCTTTTATTTCCCCAAATTGGTGAGATCTGTGGTGGGAGTGAACGGGAAGGTAATCTAGAAAAACTAGTGGAACGTTGCCAAGCAATGCAAATTGATACCCAAACACTTAACTGGTATTTAGATATGCGTAAATGGGGTTACTTTGCCTCAGCTGGCTTTGGTTTAGGGTTTGATCGTTTACTAGCTTACATCTGTGGATTGGAAAACATCCGTGATGCGATCCCCTTTCCCCGTGCCCATGGTTCCATTAACTATTAA", "aa": "MKNKRTSANIIFLEVNDGSTLLNLQAVVKQDQPELFALAESISLASAVSVSGTVALTPKSKQPLELVVKQINVLSTARADYPLQKKEHSLEFFRNNAYLRVRARTYFAIMKVRSLLSQAIFDYFFKNDFVLVHSPILTSNDCEGAGETFELKQGKEFFNKTTYLTVSGQFGAECYAQAFKKVFTFGPTFRAEKSHTSRHLSEFWMIEPEVAFANLKDLIKLIESTVKTVIKQVMQKAKQELDFLEKQFDVKLMERLKQITSTKNFHVLEYTKALEILKTAQASGQANFEVQDFNFGLDLKTEHERFLCEQHFHNQPVFVINYPKDFKAFYMKQNADGRTVGAVDLLFPQIGEICGGSEREGNLEKLVERCQAMQIDTQTLNWYLDMRKWGYFASAGFGLGFDRLLAYICGLENIRDAIPFPRAHGSINY", "aa_hexdigest": "d36182ee8d1f0330a1fb7921302367aa", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75521", "query_cov": 1.0, "subject_cov": 0.9428571428571428, "identity": 0.998, "score": 847.0, "evalue": 1.04e-309, "valid": true, "gene": "asnS", "product": "asparagine--tRNA ligase", "ec_ids": [ "6.1.1.22" ], "uniref50_id": "UniRef50_P47359", "cog_id": "COG0017", "cog_category": "J", "kegg_orthology_id": "K01893", "go_ids": [ "GO:0000166", "GO:0003676", "GO:0004816", "GO:0005524", "GO:0005737", "GO:0006421" ] }, "pscc": { "uniref50_id": "UniRef50_P47359", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47359" ], "product": "Asparagine--tRNA ligase" }, "genes": [ "asnS" ], "id": "APKECPOBEN_572", "locus": "GCA002090215_00536" }, { "type": "cds", "sequence": "contig_1", "start": 304652, "stop": 304810, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATTATTAACGGTGTCTTAATTGCCCTAGTAGCATATGGCTACTTTCACTTTAGTTTTTTAATTGTGATTGTGCTGCGTGATTTAGTGCTAGACGGATTGCGCTTTTACGCTCAGGAAAAACAGCTAATTATTCCCGCCAACCAATGGGGTAAGTGA", "aa": "MIINGVLIALVAYGYFHFSFLIVIVLRDLVLDGLRFYAQEKQLIIPANQWGK", "aa_hexdigest": "a48a692e29dc050ff208379b344ccddf", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5981.1012, "isoelectric_point": 8.16035976409912 }, "id": "APKECPOBEN_573", "locus": "GCA002090215_00537" }, { "type": "cds", "sequence": "contig_1", "start": 304916, "stop": 305017, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCATTTGCCCTATTATTTAGCTACAGCCTTCTCCTTGGTCTCGTTTGGTATTTACGCCCAACAAATATATAAAACCATTAAGGTTAAAGTAAAGTTATAA", "aa": "MHLPYYLATAFSLVSFGIYAQQIYKTIKVKVKL", "aa_hexdigest": "b18b99d24366c3ffd08f935b0d49e79d", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3835.5974000000006, "isoelectric_point": 9.778580665588379 }, "id": "APKECPOBEN_574", "locus": "GCA002090215_00538" }, { "type": "cds", "sequence": "contig_1", "start": 305039, "stop": 305512, "strand": "+", "frame": 2, "gene": "pncC", "product": "Protein MG115-like protein", "db_xrefs": [ "COG:COG1546", "COG:H", "EC:3.5.1.42", "KEGG:K03743", "RefSeq:WP_010874611.1", "SO:0001217", "UniParc:UPI000013950A", "UniRef:UniRef100_Q9EXC9", "UniRef:UniRef50_P47361", "UniRef:UniRef90_Q9EXC9" ], "nt": "GTGTACGCGCGCTTAATTGCTGAAAAACTGCTAAATCATAAGCTCACCATTGCCACTGCAGAGTCAGTTACTGGGGGCCTTTTGAGTAGTAGCTTAACCGACATCGCTGGTGCTTCTCGTTTCTTTAAGGGTGCCATTGTGGCATACAGCAATGAATTGAAAAAATCACTTTTAAATGTCAAACAATCAACCTTAATTAACCACGGTGCTGTATCGCGTTATTGTGTTCGTGAAATGGCGTTAGGTTTAATGCAAAAACTCAATGTTGATATTGCCGTGGCCTGCAGTGGGGTAGCGGGTCCTGATGCATTGGAAAACCAAGCAGTTGGTAGCTTGTTTTTCTGTGTGATTGTTGCCAACAAGGCTTATGACTTTGAAACAAAACTACCAGCTGGCTCTAGAAACGAATTGCGCCAGCTTTTTGTGCAAAAAATCTTGCAAACAGTTGAACACATTTTAAGTGAAATTTCTTAG", "aa": "MYARLIAEKLLNHKLTIATAESVTGGLLSSSLTDIAGASRFFKGAIVAYSNELKKSLLNVKQSTLINHGAVSRYCVREMALGLMQKLNVDIAVACSGVAGPDALENQAVGSLFFCVIVANKAYDFETKLPAGSRNELRQLFVQKILQTVEHILSEIS", "aa_hexdigest": "1e2c7bf2f455a48287b6ba1e2f2cb3bf", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013950A", "ncbi_nrp_id": "WP_010874611.1", "uniref100_id": "UniRef100_Q9EXC9", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013950A", "RefSeq:WP_010874611.1", "UniRef:UniRef100_Q9EXC9" ] }, "ips": { "uniref100_id": "UniRef100_Q9EXC9", "uniref90_id": "UniRef90_Q9EXC9" }, "psc": { "uniref90_id": "UniRef90_Q9EXC9", "gene": "PncC", "product": "Protein MG115 homolog", "ec_ids": [ "3.5.1.42" ], "uniref50_id": "UniRef50_P47361", "cog_id": "COG1546", "cog_category": "H", "kegg_orthology_id": "K03743" }, "pscc": { "uniref50_id": "UniRef50_P47361", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47361" ], "product": "Protein MG115" }, "genes": [ "pncC" ], "id": "APKECPOBEN_575", "locus": "GCA002090215_00539" }, { "type": "cds", "sequence": "contig_1", "start": 305538, "stop": 306293, "strand": "+", "frame": 3, "gene": "ispD", "product": "putative MG116-like protein", "db_xrefs": [ "COG:COG1211", "COG:I", "RefSeq:WP_010874612.1", "SO:0001217", "UniParc:UPI00001392BE", "UniRef:UniRef100_P75519", "UniRef:UniRef50_P75519", "UniRef:UniRef90_P75519" ], "nt": "ATGCAGCGTACTTTAAATGTTGGGGTGATCCTGTGTGAAAGCTTTATTTCCAACGCCCAAAACCCAGTTAACTCCTACATTAAGATTTACGAAAACGTGAGGATGTTTGAGTTTGCGATTAAGCTACTTCAAGAATCGCGAATTAACTTCCAAAAGATCCTTTTATACGTTTTGGAAGAGCAAATTCCACTTGTGGAAAAAGTAGTTGCCAAGTACGAAAACTGCTGGGTCTTTCGTTCCAAGCACAACGAGGTTGAGGACATTTATGAAGCCAAGGGCTTTATTGAAGACAAGTACAAAATTGGTTTAAAAAGTTCTAAAAACCAAGCCTCTTCGTATTATGACAACTGTTGTTTTGTTGTCTTGGAAGCATGTCGACCTTTAACTTCTAAGAAGGTTGTTAAAAACGTGTATGAAAAGGCAATGATTGACGGTGCCGCTGTAGCTGTACTACCGTTTGAAAGGCAGCTTGTTTGTGGTGATAATACTAAGGCGGTGCGCCAGTTAAAGGACAAGGGCAACATGAACTATTGGCGTCAAAGTAGTACTTGGGAGTTGCAATTTCCCCAGGCTTATACGCTCAATAAGTTAAACCAGTATCATAAAAACCTCTTTATGAAGGCGCGGAACATGCTTGATTTAATGAACATTTCCGCTAAGAATCCCCTAAGCATTGTCGATGGTAGTGCTTACAGCTTTCGTGTAGTTACTAGCCTGGACTTTGAAATTTTGTTAGGTATTTTGCGCAATGGATAG", "aa": "MQRTLNVGVILCESFISNAQNPVNSYIKIYENVRMFEFAIKLLQESRINFQKILLYVLEEQIPLVEKVVAKYENCWVFRSKHNEVEDIYEAKGFIEDKYKIGLKSSKNQASSYYDNCCFVVLEACRPLTSKKVVKNVYEKAMIDGAAVAVLPFERQLVCGDNTKAVRQLKDKGNMNYWRQSSTWELQFPQAYTLNKLNQYHKNLFMKARNMLDLMNISAKNPLSIVDGSAYSFRVVTSLDFEILLGILRNG", "aa_hexdigest": "ffac60a049bc33a21b2f7b4654c78aad", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001392BE", "ncbi_nrp_id": "WP_010874612.1", "uniref100_id": "UniRef100_P75519", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001392BE", "RefSeq:WP_010874612.1", "UniRef:UniRef100_P75519" ] }, "ips": { "uniref100_id": "UniRef100_P75519", "uniref90_id": "UniRef90_P75519" }, "psc": { "uniref90_id": "UniRef90_P75519", "gene": "IspD", "product": "Uncharacterized protein MG116 homolog", "uniref50_id": "UniRef50_P75519", "cog_id": "COG1211", "cog_category": "I" }, "pscc": { "uniref50_id": "UniRef50_P75519", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75519" ], "product": "Uncharacterized protein MG116 homolog" }, "genes": [ "ispD" ], "id": "APKECPOBEN_576", "locus": "GCA002090215_00540" }, { "type": "cds", "sequence": "contig_1", "start": 306286, "stop": 306510, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATAGAAAAATAGTTCAACTAATTCATAACTTTAGCGGTACAGAAAAACTAAAAGCAGCTGTTTTTTCACACGACGAACAGTGTTTCTTTGACTTTGTTAGCTTCGATGAATTAAACAGTAAGCTAACCGGGTTTCTGTTATTTGACAGCTTGGAGAAGCTGTTCAAACTAGTAGAAACAATTCAGCAAAAGCGCAGTTGGCTTTATGTTGATGAATTGTGA", "aa": "MDRKIVQLIHNFSGTEKLKAAVFSHDEQCFFDFVSFDELNSKLTGFLLFDSLEKLFKLVETIQQKRSWLYVDEL", "aa_hexdigest": "86d92816ff1c1965dd3457d22073473c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8777.983299999996, "isoelectric_point": 5.2073270797729485 }, "id": "APKECPOBEN_577", "locus": "GCA002090215_00541" }, { "type": "cds", "sequence": "contig_1", "start": 306565, "stop": 306945, "strand": "+", "frame": 1, "gene": null, "product": "MPN256 family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75518", "UniRef:UniRef90_P75518" ], "nt": "GTGAAAAAGGAGCTTGCGCAAGTCGGTGTTTTAACCCAATTGGATACTAGTTTAGTCAAGCTTTTAATGGCTTCCAAAAATACTGACAGTGCCCTTTACAACACTTACATTAAACCAGTGGAGTTGCAACAGTTTACCCAAACACCAGCTCCTGATAACGTTAATGCAGAGCAATCGCATTTAACCTTGGAGTCTACCACCGACTTGAACAACAGTCAACTAGCCAATACTCCAGCGTTGTGGGAAGTGGAACAAACGACCCAGGAATTGTTACCAACCATGGACTTTTCTAAGTTTATTGATGAATTAGATCAAATTACCAAAAACTTTTCTGATTTAGAGTTAGAACCACTTAGTTTTAACGAAGGTTTTGATGAGTGA", "aa": "MKKELAQVGVLTQLDTSLVKLLMASKNTDSALYNTYIKPVELQQFTQTPAPDNVNAEQSHLTLESTTDLNNSQLANTPALWEVEQTTQELLPTMDFSKFIDELDQITKNFSDLELEPLSFNEGFDE", "aa_hexdigest": "901fcacd9c68733468e150ab4407c3d8", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 306286, "stop": 306957, "observations": { "mutation": [ 306508, 306943 ], "stop": [ 306508, 306943 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75518", "query_cov": 0.9920634920634921, "subject_cov": 0.5605381165919282, "identity": 1.0, "score": 236.0, "evalue": 2.15e-77, "gene_start": 2, "gene_end": 126, "reference_start": 95, "reference_end": 219, "reference_sequence": "MDRKIVQLIHNFSGTEKLKAAVFSHDEQCFFDFVSFDELNSKLTGFLLFDSLEKLFKLVETIQQKRSWLYVDELWLINTATENQQLNEVSVWLVKKELAQVGVLTQLDTSLVKLLMASKNTDSALYNTYIKPVELQQFTQTPAPDNVNAEQSHLTLESTTDLNNSQLANTPALWEVEQTTQELLPTMDFSKFIDELDQITKNFSDLELEPLSFNEGFDEWNQE" }, "paralog": false, "identity": 0.9910313901345291, "score": 434.0, "evalue": 1.69e-157, "description": "Internal stop codon at 306508, 306943. Nonsense mutation around 306508, 306943.", "psc": { "uniref90_id": "UniRef90_P75518", "product": "MPN256 family protein", "uniref50_id": "UniRef50_P75518", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75518", "UniRef:UniRef50_P75518" ] }, "pscc": { "uniref50_id": "UniRef50_P75518", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75518" ], "product": "Uncharacterized protein MG117 homolog" } }, "id": "APKECPOBEN_578", "locus": "GCA002090215_00542" }, { "type": "cds", "sequence": "contig_1", "start": 306938, "stop": 307675, "strand": "+", "frame": 2, "gene": null, "product": "NAD-dependent epimerase/dehydratase family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0000557C9D" ], "nt": "ATGAGTGAAACCAAGAGTAAAGTACTCGTTTTAGGCGGTTTAGGTTACATTGGGAGCTGTTTTATTGACCAGCTTCTAAAACAGTACCCTGATGTAACTGTTAGTGTAATTGACATTAACCACACTAGTCTGGCGCTACAACTGTTACCAAGACAGGTCAACGTTCACTTTGTTAACTTACTTGACCGTGCACAGTTAACAGATACGATTGCTCAAATTAACCCTGATGTCGTGTTTCACTTTGCGGCAAAAACAAGCGTAAAGGAATCGACCGAACAACCACTTACATATTTTGACCATAACCTAGTCGGTACGCTCAACTTACTCCACGCTTTAAAAGAACTACAAAAACCGATCCAGCTGTTCTTCTCCTCCACTGCTGCAGTCTTTGGTAGTGCTAGTACACTTCCAATTCCAGAAAACTTAGTTTTAGAGGAAACACTCGCTTCCAATCCGTATGGCATTAGTAAGTTTTTAAGTGAAATAGTACTGCAAACACTTACCCGTAGCCCCCATTTTCAAGTAATAGCGTTGCGCTACTTTAACGTAGCGGGTGCTAGTAATCCCTTTGGTAACTTCAATAAAAACACGACCTTACTGATTCCTAACCTGATTAAAGCCTTTATGGAAAAGCGCACCTTCTTTCTGTATGGTGATGACTATGACACTAAAGATGGGAGCTGTATCCGTGATTACATCCATGTAGTAGACTTGTGTGATGCCCATTTGTTGGCGTGA", "aa": "MSETKSKVLVLGGLGYIGSCFIDQLLKQYPDVTVSVIDINHTSLALQLLPRQVNVHFVNLLDRAQLTDTIAQINPDVVFHFAAKTSVKESTEQPLTYFDHNLVGTLNLLHALKELQKPIQLFFSSTAAVFGSASTLPIPENLVLEETLASNPYGISKFLSEIVLQTLTRSPHFQVIALRYFNVAGASNPFGNFNKNTTLLIPNLIKAFMEKRTFFLYGDDYDTKDGSCIRDYIHVVDLCDAHLLA", "aa_hexdigest": "1c4773f5a4ecc519cd36a8a96af491eb", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0000557C9D", "query_cov": 0.8816326530612245, "subject_cov": 0.9432314410480349, "identity": 0.537, "score": 236.0, "evalue": 3.71e-75, "valid": false, "product": "NAD-dependent epimerase/dehydratase family protein", "uniref50_id": "UniRef50_UPI0000557C9D" }, "pscc": { "uniref50_id": "UniRef50_UPI0000557C9D", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0000557C9D" ], "product": "NAD-dependent epimerase/dehydratase family protein" }, "genes": [], "id": "APKECPOBEN_579", "locus": "GCA002090215_00543" }, { "type": "cds", "sequence": "contig_1", "start": 307682, "stop": 307954, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAAGCCAATCCTAAAGTGCGCTTTGAAAGCTTTAACTTAGGTAGTGGTCAGGGCTTTTCCAATTGGGAGGTCATTAATACTGCTCAAGCAATCTTTGCGCCTGAACAATTACAGCTCAAAATTGAATCACGAAGGGCTGGTGATCCACCTGTTTTAGTGGTTGATTGCACGAAGGCAAAACGCTTACTCAACTTCCAACCAACCCGCTCATTACACAAAATGCTGAGCGATGAAACAATTTTTTATCGGGATTTTTATAATAGGCTTTAA", "aa": "MQANPKVRFESFNLGSGQGFSNWEVINTAQAIFAPEQLQLKIESRRAGDPPVLVVDCTKAKRLLNFQPTRSLHKMLSDETIFYRDFYNRL", "aa_hexdigest": "bb297810e6c1c35ac8b02d6256f7e693", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10390.765999999992, "isoelectric_point": 9.355990409851074 }, "id": "APKECPOBEN_580", "locus": "GCA002090215_00544" }, { "type": "cds", "sequence": "contig_1", "start": 307990, "stop": 308913, "strand": "+", "frame": 1, "gene": null, "product": "ATP-binding cassette domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0030B82AEE" ], "nt": "ATGGAAAATATCTGCAAGAGTTTTGACAATGGTAGGGTCAAAGCTAATGTAGATGTTAACCTCACGGTTTACGAAAATACTGTCCACACATTGCTGGGTGAAAATGGTGCGGGCAAATCTACTTTAACTTCCATTTTGTTCGGTTTATACCAACCCGACAGCGGCAAAATTTTTATTGGTGAAGAGGAAGTACACTTCAAGTCCTCCAAGGATGCCGTGCAGCACAAAATTGGGATGGTGCACCAGCACTTTAAGCTGGTTGATAACTACACCGTCTTAGACAACATTATTTTGGGCAATGAAAGTAGTTTTTCCATTCCTTTTACCAACGGTAAGCTTAAACTACCTTTATTGCACCGTAAAGCTAGCGAAGCCAAAATTCAAGCAATGATGGAACGGTATGACCTCCATGTGAACTTGCACCAAAAGGTAAGCCGCTTAACGGTGGGTCAGCAACAACGGGTCGAGATCTTGAAGGTGTTATTCCGTGACAGTGACATTCTCATCTTTGATGAACCAACCGCTGTTTTGAGCGACCAGGAGATCAAGAGCTTTTTAAACATCATTAAAAACTTCAAGAAGATGGGTAAAACGATTGTGCTCATCTCCCACAAACTCAACGAAATTAAGGAAGTAGCGGAGACTGCTACCATTTTGCGTCAAGGTCACTCCGTTGGCACCTTCCAAATTAAGGACACTTCAATTGATGAAATGGCACGTTTAATGATGGGAAAGGAGTTGAAGGAAACCAAAAACAATACTCAGTTTACCGCCAAAGGTGAGCCCGTTTTAAAGGTCGAAAACCTCCATCTTTATTTAAACCAAAACTGGTTTTACAAGTTAATTGCGCGCTGGAACCAAAAGCGAATTAATCAACTCCAAAAACAGGGTAAGCCAGCGAAAACACTGTGGTTAAAGAGTTGA", "aa": "MENICKSFDNGRVKANVDVNLTVYENTVHTLLGENGAGKSTLTSILFGLYQPDSGKIFIGEEEVHFKSSKDAVQHKIGMVHQHFKLVDNYTVLDNIILGNESSFSIPFTNGKLKLPLLHRKASEAKIQAMMERYDLHVNLHQKVSRLTVGQQQRVEILKVLFRDSDILIFDEPTAVLSDQEIKSFLNIIKNFKKMGKTIVLISHKLNEIKEVAETATILRQGHSVGTFQIKDTSIDEMARLMMGKELKETKNNTQFTAKGEPVLKVENLHLYLNQNWFYKLIARWNQKRINQLQKQGKPAKTLWLKS", "aa_hexdigest": "84e353a090df7449f11743ed6071e0ca", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0030B82AEE", "query_cov": 0.8664495114006515, "subject_cov": 0.9779411764705882, "identity": 0.508, "score": 244.0, "evalue": 6.24e-77, "valid": false, "product": "ATP-binding cassette domain-containing protein", "uniref50_id": "UniRef50_UPI0030B82AEE" }, "pscc": { "uniref50_id": "UniRef50_UPI0030B82AEE", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0030B82AEE" ], "product": "ATP-binding cassette domain-containing protein" }, "genes": [], "id": "APKECPOBEN_581", "locus": "GCA002090215_00545" }, { "type": "cds", "sequence": "contig_1", "start": 308923, "stop": 309291, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00005.33" ], "nt": "TTGGCAGCTATTGAAAAAACACCCCGTTTTATTCGTGGAATCGTTAATAATTTAGGTTTTGGTAGCCAACAGGTGTTTGATAAAGGGATTAGTTTTGAGATCCATAAGGGTGAAATTTTTGCCATTGCCGGGGTGGAAGGTAACGGTCAAAACCAATTAATAGATCTTATTTGTGGCTTGGAAAAAGCAGCACCAAAAAAGGTCTTCTTCAATGGTTTTGACATTTCACGTTATTCCATTCGCAAGCGGATTAACGCTGGTATTGGTTTTGTGTTAGAAGACCGCCATAAATACGGATTAATCTTAGACCAAACGGTGCGTTTTAACGCGGTGAATAACCAAATTGATCGTAAGCAATTTAGCAGTTGA", "aa": "MAAIEKTPRFIRGIVNNLGFGSQQVFDKGISFEIHKGEIFAIAGVEGNGQNQLIDLICGLEKAAPKKVFFNGFDISRYSIRKRINAGIGFVLEDRHKYGLILDQTVRFNAVNNQIDRKQFSS", "aa_hexdigest": "ed13da761070c5bc4fd52bc221665a26", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00005.33", "name": "ABC_tran", "length": 82, "aa_cov": 0.6721311475409836, "hmm_cov": 0.9512195121951219, "evalue": 3.773538132962372e-09, "score": 32.75098419189453, "start": 28, "stop": 109 } ], "seq_stats": { "molecular_weight": 13676.539799999979, "isoelectric_point": 9.607094764709473 }, "id": "APKECPOBEN_582", "locus": "GCA002090215_00546" }, { "type": "cds", "sequence": "contig_1", "start": 309307, "stop": 309687, "strand": "+", "frame": 1, "gene": "mglA", "product": "Galactose/methyl galactoside import ATP-binding protein MglA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A223M965" ], "nt": "ATGCAAATAGCTGTTTACACTAACCAAATCGTCGATAAATTTGATGTTCGTGGAGCGGTACAAGGTACAGCAATAGTGCGTTTACTTTCCGGGGGTAATCAGCAAAAGCTGATTATTGGTCGGGAACTGACAAAGCAGAACGAGCTGTTAGTGTTTGCTCAAGTAACCCGGGGTTTGGATGTTGGTGCCATTTCGTTTATCCACCAAAAGATTTTGGATGCAAAGAAACAAAATAATGCAATCTTGTTAGTGTCATATGAGTTAGATGAAATTTTAGCTATTGCCGATACCATTGGCGTAATTAACAAAGGCAACCTGTTGGAAGTCAATAAACGTGATGTAATGACGCGTGAACGCATAGGCAAACTAATCATGCAATAA", "aa": "MQIAVYTNQIVDKFDVRGAVQGTAIVRLLSGGNQQKLIIGRELTKQNELLVFAQVTRGLDVGAISFIHQKILDAKKQNNAILLVSYELDEILAIADTIGVINKGNLLEVNKRDVMTRERIGKLIMQ", "aa_hexdigest": "5ac53fec40fc3a7da533f40dc03ef0b4", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A223M965", "query_cov": 0.9206349206349206, "subject_cov": 0.9354838709677419, "identity": 0.5429999999999999, "score": 126.0, "evalue": 4.14e-35, "valid": false, "product": "Galactose/methyl galactoside import ATP-binding protein MglA", "uniref50_id": "UniRef50_A0A223M965" }, "pscc": { "uniref50_id": "UniRef50_A0A223M965", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A223M965" ], "product": "Galactose/methyl galactoside import ATP-binding protein MglA" }, "genes": [ "mglA" ], "id": "APKECPOBEN_583", "locus": "GCA002090215_00547" }, { "type": "cds", "sequence": "contig_1", "start": 309704, "stop": 310360, "strand": "+", "frame": 2, "gene": "nupP", "product": "putative MG120-like protein", "db_xrefs": [ "COG:COG4603", "COG:F", "GO:0005886", "GO:0022857", "SO:0001217", "UniRef:UniRef50_P75515", "UniRef:UniRef90_P75515" ], "nt": "ATGAATGCTTTAAATTACCTCAAACACCGTTTTCTGTTTAGTAAAGACAAGTTTTGGTACGCCCCTTTTAAACAAAAGCAACGTCGGAGCATCTACTCCACCTTTTCGTTGATTGTTCTCTCCTTTATTGTTTCGTTCTTTTTAATAGTCGCCATTCCCGGCATTAAAGGCGGTACCTTCTTAGAAATCTTTACCCGTTTATTTAAAGATAGGATTAACATCGAAAACTTTGCACGACAAATTGCGATTTACACCCTAGCTGCTTTGGCGTTTAGCTTTTGTATGAGTGTTGGGGTGTTTAACATTGGGATTTCGGGACAAATGATGGCTGGCGCTAATTTTGGTTTCATGATGATCCTCAAGGTTTTCCCCGAGTCATTCCGTCCTGCCTTTGGTGGTCAGATCATTACCATCCTCTTAATGATTCTAGGTAGTGTGACCGTTGCTATGGTGGTAGCTGCTTTAAAGGTCTTCTTTAAGGTTAATGAAGTGGTTAGTGCCATTATGCTCAACTGGGTAATTGTGCTGGTTAGCGCTTATCTAGTGGGTACTTACATTAAACCGGAAAAAACTGATACTTCCCAGTTTTACTCGATTGAACTACCGGATGCCTTTGCGCTGTATAACTTCTCTGATGTTCAACAAAAGTATGGTTGA", "aa": "MNALNYLKHRFLFSKDKFWYAPFKQKQRRSIYSTFSLIVLSFIVSFFLIVAIPGIKGGTFLEIFTRLFKDRINIENFARQIAIYTLAALAFSFCMSVGVFNIGISGQMMAGANFGFMMILKVFPESFRPAFGGQIITILLMILGSVTVAMVVAALKVFFKVNEVVSAIMLNWVIVLVSAYLVGTYIKPEKTDTSQFYSIELPDAFALYNFSDVQQKYG", "aa_hexdigest": "2f79f5bfd5b1582b525e48dc57e9a1a4", "start_type": "ATG", "rbs_motif": null, "genes": [ "nupP" ], "truncated": "3-prime", "pseudogene": { "start": 309704, "stop": 310663, "observations": { "mutation": [ 310358 ], "stop": [ 310358 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75515", "query_cov": 1.0, "subject_cov": 0.42166344294003866, "identity": 1.0, "score": 412.0, "evalue": 7.48e-141, "gene_start": 1, "gene_end": 218, "reference_start": 1, "reference_end": 218, "reference_sequence": "MNALNYLKHRFLFSKDKFWYAPFKQKQRRSIYSTFSLIVLSFIVSFFLIVAIPGIKGGTFLEIFTRLFKDRINIENFARQIAIYTLAALAFSFCMSVGVFNIGISGQMMAGANFGFMMILKVFPESFRPAFGGQIITILLMILGSVTVAMVVAALKVFFKVNEVVSAIMLNWVIVLVSAYLVGTYIKPEKTDTSQFYSIELPDAFALYNFSDVQQKYGWLTSLVIAIAAAIFIAVLMKFTVFGHKLKSTGLSVTGSQAAGYSVKKYQFLSFVISGILSGLLAAVVYTASFEKQLTFSDVGDFGITSVPITGFDGIAIGLIALNSPARIVIVSTIISFVTIGAKPAGLNAATASLVLGIMMYFAAIYNLMIYIKPWRMIVKLNISKMNEAAYDEFQNEMAANLETLSFQRFLDKQKRKHDKERMVWFDTKRFEEYQKKKQATLQTFHENSSQNLLQYWKQQLLVADVKRLTFKWDFLTFKHQQKYILRWYKGKNKKQTALENEFASLNEAISQKLEEK" }, "paralog": false, "identity": 0.9968652037617555, "score": 610.0, "evalue": 4.96e-221, "description": "Internal stop codon at 310358. Nonsense mutation around 310358.", "psc": { "uniref90_id": "UniRef90_P75515", "gene": "NupP", "product": "Uncharacterized protein MG120 homolog", "uniref50_id": "UniRef50_P75515", "cog_id": "COG4603", "cog_category": "F", "go_ids": [ "GO:0005886", "GO:0022857" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75515", "UniRef:UniRef50_P75515", "COG:COG4603", "COG:F", "GO:0005886", "GO:0022857" ] }, "pscc": { "uniref50_id": "UniRef50_P75515", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75515" ], "product": "Uncharacterized protein MG120 homolog" } }, "id": "APKECPOBEN_584", "locus": "GCA002090215_00548" }, { "type": "cds", "sequence": "contig_1", "start": 310370, "stop": 311074, "strand": "+", "frame": 2, "gene": "nupP", "product": "putative MG120-like protein", "db_xrefs": [ "COG:COG4603", "COG:F", "GO:0005886", "GO:0022857", "SO:0001217", "UniRef:UniRef50_P75515", "UniRef:UniRef90_P75515" ], "nt": "TTGGTTATTGCCATAGCAGCTGCCATATTCATTGCAGTTTTAATGAAGTTCACCGTTTTTGGACACAAACTCAAGTCCACGGGTTTAAGTGTGACGGGTTCGCAAGCCGCTGGTTACAGTGTTAAGAAGTATCAGTTCCTCTCGTTTGTGATTTCAGGAATCCTTTCTGGTTTACTAGCAGCTGTAGTTTATACCGCATCCTTTGAAAAGCAGTTAACGTTCTCAGATGTGGGTGACTTTGGGATTACATCGGTACCGATTACTGGGTTTGATGGAATTGCAATTGGTTTAATTGCGCTCAACAGTCCAGCGCGAATTGTAATAGTTTCCACCATTATTTCATTTGTAACCATTGGTGCTAAACCAGCGGGGTTAAACGCTGCTACTGCGAGCTTGGTGTTGGGTATCATGATGTATTTCGCTGCCATTTATAACCTGATGATTTACATCAAGCCATGGCGCATGATTGTCAAGCTCAATATTAGTAAGATGAACGAAGCGGCATATGATGAATTCCAAAACGAGATGGCTGCTAATTTAGAAACGCTGTCGTTCCAGCGCTTTTTAGATAAACAAAAACGGAAGCATGATAAAGAGCGAATGGTTTGGTTTGACACCAAGCGCTTTGAGGAGTACCAGAAAAAGAAGCAAGCAACTCTACAAACCTTCCATGAAAATTCCTCGCAAAACTTACTGCAGTACTGA", "aa": "MVIAIAAAIFIAVLMKFTVFGHKLKSTGLSVTGSQAAGYSVKKYQFLSFVISGILSGLLAAVVYTASFEKQLTFSDVGDFGITSVPITGFDGIAIGLIALNSPARIVIVSTIISFVTIGAKPAGLNAATASLVLGIMMYFAAIYNLMIYIKPWRMIVKLNISKMNEAAYDEFQNEMAANLETLSFQRFLDKQKRKHDKERMVWFDTKRFEEYQKKKQATLQTFHENSSQNLLQY", "aa_hexdigest": "8f60c02760862912bcd61cb348b9d4a7", "start_type": "TTG", "rbs_motif": null, "genes": [ "nupP" ], "truncated": "both", "pseudogene": { "start": 310070, "stop": 311257, "observations": { "mutation": [ 310358, 311072, 311120 ], "stop": [ 310358, 311072, 311120 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75515", "query_cov": 1.0, "subject_cov": 0.4526112185686654, "identity": 0.996, "score": 430.0, "evalue": 1.07e-147, "gene_start": 1, "gene_end": 234, "reference_start": 223, "reference_end": 456, "reference_sequence": "MNALNYLKHRFLFSKDKFWYAPFKQKQRRSIYSTFSLIVLSFIVSFFLIVAIPGIKGGTFLEIFTRLFKDRINIENFARQIAIYTLAALAFSFCMSVGVFNIGISGQMMAGANFGFMMILKVFPESFRPAFGGQIITILLMILGSVTVAMVVAALKVFFKVNEVVSAIMLNWVIVLVSAYLVGTYIKPEKTDTSQFYSIELPDAFALYNFSDVQQKYGWLTSLVIAIAAAIFIAVLMKFTVFGHKLKSTGLSVTGSQAAGYSVKKYQFLSFVISGILSGLLAAVVYTASFEKQLTFSDVGDFGITSVPITGFDGIAIGLIALNSPARIVIVSTIISFVTIGAKPAGLNAATASLVLGIMMYFAAIYNLMIYIKPWRMIVKLNISKMNEAAYDEFQNEMAANLETLSFQRFLDKQKRKHDKERMVWFDTKRFEEYQKKKQATLQTFHENSSQNLLQYWKQQLLVADVKRLTFKWDFLTFKHQQKYILRWYKGKNKKQTALENEFASLNEAISQKLEEK" }, "paralog": false, "identity": 0.9924050632911392, "score": 751.0, "evalue": 1.39e-276, "description": "Internal stop codon at 310358, 311072, 311120. Nonsense mutation around 310358, 311072, 311120.", "psc": { "uniref90_id": "UniRef90_P75515", "gene": "NupP", "product": "Uncharacterized protein MG120 homolog", "uniref50_id": "UniRef50_P75515", "cog_id": "COG4603", "cog_category": "F", "go_ids": [ "GO:0005886", "GO:0022857" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75515", "UniRef:UniRef50_P75515", "COG:COG4603", "COG:F", "GO:0005886", "GO:0022857" ] }, "pscc": { "uniref50_id": "UniRef50_P75515", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75515" ], "product": "Uncharacterized protein MG120 homolog" } }, "id": "APKECPOBEN_585", "locus": "GCA002090215_00549" }, { "type": "cds", "sequence": "contig_1", "start": 311257, "stop": 311466, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCACAGTCTTTAATTTCTTTTAGTAACTTAGATAACTGGTTGTTTGTCGCTCCTGCACTGCTACTCGCTGTATTGAGTGGTTACTTATCTGAGCGGGTTGGTATTGTCAATATTGCCATTAACGGGGGCATGGTGTTTGGCGGGATGTTCTTATCCTTAATGTCTTATGCCTTTGTGCCCAATGCAAATGATTCTGCACCATCTTGA", "aa": "MSQSLISFSNLDNWLFVAPALLLAVLSGYLSERVGIVNIAINGGMVFGGMFLSLMSYAFVPNANDSAPS", "aa_hexdigest": "c9258b06627954a98cba854935a5f624", "start_type": "ATG", "rbs_motif": "AAAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7300.407999999999, "isoelectric_point": 4.0500284194946286 }, "id": "APKECPOBEN_586", "locus": "GCA002090215_00550" }, { "type": "cds", "sequence": "contig_1", "start": 311560, "stop": 311991, "strand": "+", "frame": 1, "gene": "nupQ", "product": "putative MG121-like protein", "db_xrefs": [ "COG:COG1079", "COG:F", "GO:0005886", "GO:0022857", "KEGG:K23536", "SO:0001217", "UniRef:UniRef50_P47367", "UniRef:UniRef90_P75514" ], "nt": "GTGATTGTTGGCACAGGGGTTAACTTACTGGGCACGGGAATTAATTTCTTTGTGGCACAAAATGCCCGCTCATTGCTTAACGATACTGATTTAAGGGTACGATACAGCTTTGTAAGAACAGGAAACTCTGTAAGTATTGAGGGTATAGCAATCTTTGCCTTTGCAATTATCTTTGTTTTATTAGTGTGGTATTTGATGAACTTTACTAAAACGGGCTTGCGTTACCGTGCTGTTGGGGAAAATCCTAATGTGATTGATACCCAAGGTATTAGTGTGTATAAATACCAGTGGTTTGGGGTAATGGCTTCGACGATGGTAGCAGCTTTAGCGGGTTGTTGTTTTGCTTTAAGTCCACAGGTACCTAGCTTTTCTAGCGGGGATGTGAGCGGGTTTGGTTTTATTGCCATTGCCATTATGATCATTTCGATGTGA", "aa": "MIVGTGVNLLGTGINFFVAQNARSLLNDTDLRVRYSFVRTGNSVSIEGIAIFAFAIIFVLLVWYLMNFTKTGLRYRAVGENPNVIDTQGISVYKYQWFGVMASTMVAALAGCCFALSPQVPSFSSGDVSGFGFIAIAIMIISM", "aa_hexdigest": "acee666409cc5e121e0b09cfee537c26", "start_type": "GTG", "rbs_motif": null, "genes": [ "nupQ" ], "truncated": "both", "pseudogene": { "start": 311260, "stop": 312192, "observations": { "mutation": [ 311464, 311989 ], "stop": [ 311464, 311989 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75514", "query_cov": 1.0, "subject_cov": 0.45980707395498394, "identity": 0.993, "score": 278.0, "evalue": 4.11e-92, "gene_start": 1, "gene_end": 143, "reference_start": 102, "reference_end": 244, "reference_sequence": "MSQSLISFSNLDNWLFVAPALLLAVLSGYLSERVGIVNIAINGGMVFGGMFLSLMSYAFVPNANDSAPSWSLAISIPLSVIFASAVGFLFGIAAIKLKADHVIVGTGVNLLGTGINFFVAQNARSLLNDTDLRVRYSFVRTGNSVSIEGIAIFAFAIIFVLLVWYLMNFTKTGLRYRAVGENPNVIDTQGISVYKYQWFGVMASTMVAALAGCCFALSPQVPSFSSGDVSGFGFIAIAIMIISMWRIIPSIVISPLFALAYVLTTGVVGNANNTYLLRTIPFIISLLVMMVFGYLNVGPKNVGKHFDKGLR" }, "paralog": false, "identity": 0.9935483870967742, "score": 594.0, "evalue": 3.54e-219, "description": "Internal stop codon at 311464, 311989. Nonsense mutation around 311464, 311989.", "psc": { "uniref90_id": "UniRef90_P75514", "gene": "NupQ", "product": "Uncharacterized protein MG121 homolog", "uniref50_id": "UniRef50_P47367", "cog_id": "COG1079", "cog_category": "F", "kegg_orthology_id": "K23536", "go_ids": [ "GO:0005886", "GO:0022857" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75514", "UniRef:UniRef50_P47367", "COG:COG1079", "COG:F", "KEGG:K23536", "GO:0005886", "GO:0022857" ] }, "pscc": { "uniref50_id": "UniRef50_P47367", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47367" ], "product": "Uncharacterized protein MG121" } }, "id": "APKECPOBEN_587", "locus": "GCA002090215_00551" }, { "type": "cds", "sequence": "contig_1", "start": 312010, "stop": 312192, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATCTCGCCCTTGTTTGCACTAGCTTATGTGCTTACTACCGGTGTGGTAGGTAATGCTAATAACACTTACTTATTGAGAACTATTCCGTTTATTATTTCGCTGTTGGTAATGATGGTATTTGGATATCTCAATGTAGGGCCGAAAAACGTTGGTAAGCATTTCGATAAGGGTTTGCGATAG", "aa": "MISPLFALAYVLTTGVVGNANNTYLLRTIPFIISLLVMMVFGYLNVGPKNVGKHFDKGLR", "aa_hexdigest": "c3c0b9758f28338dd47dce61416b593b", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6599.869200000001, "isoelectric_point": 9.99467868804932 }, "id": "APKECPOBEN_588", "locus": "GCA002090215_00552" }, { "type": "cds", "sequence": "contig_1", "start": 312229, "stop": 312396, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTAAAAATCTGGTGGTGATTGAATCACCCAATAAAGTTAAAACGTTGCAAAAATATTTACCGAATGACTTTGAAATAGTCTCTACCATTGGTCATATTCGGGAAATGGTGCACAAAAACTTTGGTTTTAACGAAGCCGATTACTCACCAGTTTGGGAAGACTGA", "aa": "MSKNLVVIESPNKVKTLQKYLPNDFEIVSTIGHIREMVHKNFGFNEADYSPVWED", "aa_hexdigest": "233f7614c4ddde2fe0020814a1fc0412", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6409.217500000001, "isoelectric_point": 5.53830051422119 }, "id": "APKECPOBEN_589", "locus": "GCA002090215_00553" }, { "type": "cds", "sequence": "contig_1", "start": 312580, "stop": 312861, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGATGAAAAGGACAAGTCGAAGTGCCAACGCATTACCTTTAACGAAATTACCAAGAACGCCGTTTTAGATGCACTGAAAAACCCACGGGAAATTGATCAGAGTTGGGTGCAGAGTCAGTTTGCGCGCCAAATTCTTGACCGGATGATTGGTTTTCGCCTTTCAAGGCTGTTAAACAATTACCTCTCAGCTAAGTCAGCGGGACGGGTACAATCAGTAGCACTGCGTTTTTTAGAAGAACGTGAACAGGAAATTCGTAGCTTTGTACCACGGTTTTGGTGA", "aa": "MDEKDKSKCQRITFNEITKNAVLDALKNPREIDQSWVQSQFARQILDRMIGFRLSRLLNNYLSAKSAGRVQSVALRFLEEREQEIRSFVPRFW", "aa_hexdigest": "86088825daf4df9ad4be62be951e9cf5", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11062.560499999992, "isoelectric_point": 9.883535194396973 }, "id": "APKECPOBEN_590", "locus": "GCA002090215_00554" }, { "type": "cds", "sequence": "contig_1", "start": 312871, "stop": 314364, "strand": "+", "frame": 1, "gene": "topA", "product": "type I DNA topoisomerase", "db_xrefs": [ "COG:COG0551", "COG:L", "EC:5.6.2.1", "GO:0003677", "GO:0003917", "GO:0005694", "GO:0005737", "GO:0006260", "GO:0006265", "GO:0046872", "KEGG:K03168", "SO:0001217", "UniRef:UniRef50_P47368", "UniRef:UniRef90_P78032" ], "nt": "GTGTTGTTAAATCCCAAGGCTGAAGGTGTTAGGGAGGCGTGTGCTAATCGTTCAATTCCAATTGTCTTGCGTGAAATTAATCCTGCTTTACGTGCTGGCTTGAAATTTGAAGAGGAGAAGAGCGTATCGGGAATTGACTTTTTAGATGAAGCATCAGCTAAAAAGTTTGGTGAACAGCTCAAGGGTGTCTTTGAGGTGTACAACATTGATGAAACCAAACACTACAGCAGTTCGCCCAATTCCGCTTACACCACCGCTTCATTGCAAAAGGATGCCATCAACAAACTAGGTTGGTCTTCCAAAAAGGTAACCTTAATTGCCCAACACCTGTATGAAGGGGTGAGTATTAATGGAGAACAAACCGCTTTAATTAGCTATCCCCGTACCGACTCAACTCGTTTATCAGCGCAATTTCAACAAAGTTGTAAAGAATACATCTTAAACCATTACGGAGAAAAGTACCTCTCTAATCGGATTGTAAGCGCTAAGGGAAAAAAAGGTGAAAAAATTATCCAAGACGCCCATGAAGCAATTCACCCAACTGACATTAACATTACCCCGGAAATGGTCAAGAATGCGATTAAGAAGGATGAGTTCTTACTGTACCGCTTAATCTGGATCCGTACGGTCGCTAGTTTAATGGCGGATTGTAAAAAATCACATACCCACATCCGGTTTATTAACGATGGTAATAAGTTTTACGCTTCTTCCAAGTCATTAGTATTTGATGGTTATCGCAAAATTTACGAACACTTTGAAAATAAGGAAAGTAATGATCTCTACATTGATTTGGATAAGATTAGGGTTGGTGATCGTTTCATGGCTAAAGACATTAAGATCACAGCCCGCCAAACCCATCCAGCAGCCCGTTATACCCAAGCTAGTTTAATTGAAGCGTTAGAAAAGTCCAATATTGGTCGACCTTCCACCTATAACACGATGGCTAGTGTTAATTTAGACCGGGGTTATGCTAGTTTAAATAAACATGCTTTTCACGTAACACAACTAGGGGAGCAGGTTAATGAAGAATTGTCCAAACACTTTGGTAAGATCATTAACAAAGAGTTCACTAAAAACATGGAGAAGTCTTTGGATGAAATTGCTGAAAATAAAAAGAACTACCAGGAGTTTCTCCGTGATTTTTGGTCAAACTTTAAGGAAGAGGTAAAGCTAGCGGAGGGTTCGATTCAAAGGGTGAAAAAGGAAAAGGAATTTGTTGGTCGTGATTGTCCAAGCTGTGCTAGTCCCTTGTTGTACCGCTATACTAAACGGGGTAATGAAAAGTTTGTTGGGTGCAGTAACTTTCCCAACTGTAAATACAACGAGTTTAGCCAAAATAAACCGAATTTAACTTTGGAAAAGCTCGAAGAACTGTGTCCGGAGTGTAACAGCCAACTAGTGAAAAGACGCACTAAGTTTAATCCCAATAAAACCTTTGTTGGTTGTAGTAATTTCCCTAGGTGCCGCTACATAAAGAAGGATAATGCTAGCTAG", "aa": "MLLNPKAEGVREACANRSIPIVLREINPALRAGLKFEEEKSVSGIDFLDEASAKKFGEQLKGVFEVYNIDETKHYSSSPNSAYTTASLQKDAINKLGWSSKKVTLIAQHLYEGVSINGEQTALISYPRTDSTRLSAQFQQSCKEYILNHYGEKYLSNRIVSAKGKKGEKIIQDAHEAIHPTDINITPEMVKNAIKKDEFLLYRLIWIRTVASLMADCKKSHTHIRFINDGNKFYASSKSLVFDGYRKIYEHFENKESNDLYIDLDKIRVGDRFMAKDIKITARQTHPAARYTQASLIEALEKSNIGRPSTYNTMASVNLDRGYASLNKHAFHVTQLGEQVNEELSKHFGKIINKEFTKNMEKSLDEIAENKKNYQEFLRDFWSNFKEEVKLAEGSIQRVKKEKEFVGRDCPSCASPLLYRYTKRGNEKFVGCSNFPNCKYNEFSQNKPNLTLEKLEELCPECNSQLVKRRTKFNPNKTFVGCSNFPRCRYIKKDNAS", "aa_hexdigest": "d153bf82eca56e7c6923e927a752f89b", "start_type": "GTG", "rbs_motif": null, "genes": [ "topA" ], "truncated": "5-prime", "pseudogene": { "start": 312571, "stop": 314364, "observations": { "mutation": [ 312859 ], "stop": [ 312859 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P78032", "query_cov": 1.0, "subject_cov": 0.69901547116737, "identity": 0.998, "score": 934.0, "evalue": 0.0, "gene_start": 1, "gene_end": 497, "reference_start": 215, "reference_end": 711, "reference_sequence": "MSKNLVVIESPNKVKTLQKYLPNDFEIVSTIGHIREMVHKNFGFNEADYSPVWEDWTKSKKKFSSLSFKGNLKGKKLLSKYDIIKSIKEKASKATNIYLATDPDREGEAISWHVYDVLDEKDKSKCQRITFNEITKNAVLDALKNPREIDQSWVQSQFARQILDRMIGFRLSRLLNNYLSAKSAGRVQSVALRFLEEREQEIRSFVPRFWWTLDVLLNPKAEGVREACANRSIPIVLREINPALRAGLKFEEEKSVSGIDFLDEASAKKFGEQLKGVFEVYNIDETKHYSSSPNSAYTTASLQKDAINKLGWSSKKVTLIAQHLYEGVSINGEQTALISYPRTDSTRLSAQFQQSCKEYILNHYGEKYLSNRIVSAKGKKGEKIIQDAHEAIHPTDINITPEMVKNAIKKDEFLLYRLIWIRTVASLMADCKKSHTHIRFINDGNKFYASSKSLVFDGYRKIYEHFENKESNDLYIDLDKIRVGDRFMAKDIKITARQTHPAARYTQASLIEALEKSNIGRPSTYNTMASVNLDRGYASLNKHAFHVTQLGEQVNEELSKHFGKIINKEFTKNMEKSLDEIAENKKNYQEFLRDFWSNFKEEVKLAEGSIQRVKKEKEFVGRDCPSCASPLLYRYTKRGNEKFVGCSNFPNCKYNEFSQNKPNLTLEKLEELCPECNSQLVKRRTKFNPNKTFVGCSNFPRCRYIKKDNAS" }, "paralog": false, "identity": 0.998324958123953, "score": 1132.0, "evalue": 0.0, "description": "Internal stop codon at 312859. Nonsense mutation around 312859.", "psc": { "uniref90_id": "UniRef90_P78032", "gene": "topA", "product": "type I DNA topoisomerase", "ec_ids": [ "5.6.2.1" ], "uniref50_id": "UniRef50_P47368", "cog_id": "COG0551", "cog_category": "L", "kegg_orthology_id": "K03168", "go_ids": [ "GO:0003677", "GO:0003917", "GO:0005694", "GO:0005737", "GO:0006260", "GO:0006265", "GO:0046872" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P78032", "EC:5.6.2.1", "UniRef:UniRef50_P47368", "COG:COG0551", "COG:L", "KEGG:K03168", "GO:0003677", "GO:0003917", "GO:0005694", "GO:0005737", "GO:0006260", "GO:0006265", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_P47368", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47368" ], "product": "DNA topoisomerase 1" } }, "id": "APKECPOBEN_591", "locus": "GCA002090215_00555" }, { "type": "cds", "sequence": "contig_1", "start": 314266, "stop": 314547, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATCTTGTGCTTAATTTGTTCCGGCTCTAAGTGGTACTTTGTTCGTTTCTTGTTGTTTTTGAGGTGTAAATTTCAAGCAAAGAAAATGGTGATAATAAGCCAAATGAGGATACTTAAGATCAAAATGGCACTTACCAACGCTCAAATTATTGAGAAGGAAGTGGCACTTCTGCTCGTTAAACTAGCTAGCATTATCCTTCTTTATGTAGCGGCACCTAGGGAAATTACTACAACCAACAAAGGTTTTATTGGGATTAAACTTAGTGCGTCTTTTCACTAG", "aa": "MILCLICSGSKWYFVRFLLFLRCKFQAKKMVIISQMRILKIKMALTNAQIIEKEVALLLVKLASIILLYVAAPREITTTNKGFIGIKLSASFH", "aa_hexdigest": "a8d04ad9ca7cd0f20adbfb5ed441c8f3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10606.048899999985, "isoelectric_point": 10.166229057312012 }, "id": "APKECPOBEN_592", "locus": "GCA002090215_00556" }, { "type": "cds", "sequence": "contig_1", "start": 314573, "stop": 314827, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGGACTTTTACCAACAGCAGATTAACACTACTGCAACTGAGCTGAAGTGGTTGGATGGTCAGTTTCAGCAAATTGATGAAACCGATAAAAAGAAAGCGCATCAAATTGCAATTAGACTAGCGCGGAACCAACTGTTACAACAGCTTAGTGTAAAGTTAGACCAAAAGCAGTTTAGTCAACGCGCTAATAATGAACTGCAAAAGTTAAAGTTGTCTAACTTAGAGAGCTTTACTAACCAAAAGATTAAATGA", "aa": "MLDFYQQQINTTATELKWLDGQFQQIDETDKKKAHQIAIRLARNQLLQQLSVKLDQKQFSQRANNELQKLKLSNLESFTNQKIK", "aa_hexdigest": "ebb8dcd416668415c94c865c1f2b32ba", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9962.235299999993, "isoelectric_point": 9.572410774230956 }, "id": "APKECPOBEN_593", "locus": "GCA002090215_00557" }, { "type": "cds", "sequence": "contig_1", "start": 315005, "stop": 315304, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAAGACTGCAAGCAAAAAACCTGTTTAATAAGTTAATTGCTCAAAGTAGCAGTGTCAAGATGAGCGAAAAACTGATTAACAATGCTTTGCAATTAGTTCAGGACAATTTGGCATTACAAGCGAGTGAAAGTGGCAATAAGCTGCTCAAGGAGTTTGAATTATCGTGCACTAACACACAGCTAGTCCAACTGTTGGGCTTTCAGCAGTTTTACTTCGGTACAAACCTGCTTAGCTTACTTGATTTGAGCCGTTCAATCGCTGTTTTAGTGCGCTTTTTAAATGAGCACTGTAAGTGA", "aa": "MKRLQAKNLFNKLIAQSSSVKMSEKLINNALQLVQDNLALQASESGNKLLKEFELSCTNTQLVQLLGFQQFYFGTNLLSLLDLSRSIAVLVRFLNEHCK", "aa_hexdigest": "3b3dc50dad6cd8181041ef113159253d", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11202.95829999999, "isoelectric_point": 9.342516517639162 }, "id": "APKECPOBEN_594", "locus": "GCA002090215_00558" }, { "type": "cds", "sequence": "contig_1", "start": 315326, "stop": 315781, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGAAACTGCTTTGTTTAACAACTTGCAATGGGTTAATAACAACGACTTCTTTTTAAAGTCACACAATGATTTAAAACAGCTGCACCTCTCCGCTGAACAGTTGGCGATTATTGAACAGCAAAACCGTCCCTTTTACATTGATGCTTATGCTTTATTGATTGCTGGCGTAAAGCAAATGCTGATGGAGCACGATGCTGTGGAACCAAAACAAATTCACTTTCATAATGCCAAAAAGGTAATGGAAAGCTTCCAGCTGTTTGGCATTGACCAGTTAGCGTTAATAGAGTACAACAACTGTCTCTATGGCTTTGTCACCACTAAACTCTATGAGATTAAACAATTAGATGATTTAGCTTTATTTAAAGTTTTGTTTAAAAGCTTTTTGAACAAGCATTTGAAACAAAAATTTGCAACAATCAGTTTATTTGTAAATACCCAAACACTCATGATCTAA", "aa": "METALFNNLQWVNNNDFFLKSHNDLKQLHLSAEQLAIIEQQNRPFYIDAYALLIAGVKQMLMEHDAVEPKQIHFHNAKKVMESFQLFGIDQLALIEYNNCLYGFVTTKLYEIKQLDDLALFKVLFKSFLNKHLKQKFATISLFVNTQTLMI", "aa_hexdigest": "2a1997b59ce9fb766075ff61232ee127", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17700.409200000002, "isoelectric_point": 6.579590797424316 }, "id": "APKECPOBEN_595", "locus": "GCA002090215_00559" }, { "type": "cds", "sequence": "contig_1", "start": 315985, "stop": 316155, "strand": "+", "frame": 1, "gene": "trxA", "product": "thioredoxin", "db_xrefs": [ "COG:COG3118", "COG:O", "GO:0005737", "GO:0015035", "SO:0001217", "UniRef:UniRef50_P47370", "UniRef:UniRef90_P75512" ], "nt": "GTGAGCACTGTAGCGTTTGCCAAGGTAAATGTCGATGAACAAACTGACATTGCCGCTGCTTACAAAATTACCTCACTACCTACAATTGTTTTATTTGAAAAAGGCCAGGAAAAGCACCGTGCTATTGGTTTTATGCCTAAAGCCAAGATCGTGCAACTTGTCAGCCAATAA", "aa": "MSTVAFAKVNVDEQTDIAAAYKITSLPTIVLFEKGQEKHRAIGFMPKAKIVQLVSQ", "aa_hexdigest": "96c9b5bd4ff0c3b29b76367e283794c2", "start_type": "GTG", "rbs_motif": null, "genes": [ "trxA" ], "truncated": "5-prime", "pseudogene": { "start": 315847, "stop": 316155, "observations": { "mutation": [ 315922 ], "stop": [ 315922 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75512", "query_cov": 1.0, "subject_cov": 0.5490196078431373, "identity": 0.982, "score": 107.0, "evalue": 5.32e-29, "gene_start": 1, "gene_end": 56, "reference_start": 47, "reference_end": 102, "reference_sequence": "MVTEIKSLKQLGELFASNNKVIIDFWAEWCGPCKITGPEFAKAASEVSTVAFAKVNVDEQTDIAAAYKITSLPTIVLFEKGQEKHRAIGFMPKAKIVQLVSQ" }, "paralog": false, "identity": 0.9901960784313726, "score": 198.0, "evalue": 2.28e-67, "description": "Internal stop codon at 315922. Nonsense mutation around 315922.", "psc": { "uniref90_id": "UniRef90_P75512", "gene": "trxA", "product": "thioredoxin", "uniref50_id": "UniRef50_P47370", "cog_id": "COG3118", "cog_category": "O", "go_ids": [ "GO:0005737", "GO:0015035" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75512", "UniRef:UniRef50_P47370", "COG:COG3118", "COG:O", "GO:0005737", "GO:0015035" ] }, "pscc": { "uniref50_id": "UniRef50_P47370", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47370" ], "product": "Thioredoxin" } }, "id": "APKECPOBEN_596", "locus": "GCA002090215_00560" }, { "type": "cds", "sequence": "contig_1", "start": 316167, "stop": 316571, "strand": "+", "frame": 3, "gene": "cof", "product": "Putative phosphatase MPN_264", "db_xrefs": [ "COG:COG0561", "COG:HR", "EC:3.1.3.-", "GO:0000287", "GO:0005829", "GO:0016791", "SO:0001217", "UniRef:UniRef50_P47371", "UniRef:UniRef90_P75511" ], "nt": "ATGATTGACTTGCTCGGGTTAGATCTTGATGGCACTTTGTTGTCTAGAACAAGGCAAATTAACGATCCTACTAAACAAGCTTTGGCTAATTTAATCCAAAAGAAGCCTAGCTTAAAGGTAATGATTCTAACCGGTAGATCATTATTCTCCACCTTGAAGTACGTTCAAGAGCTCAACGAGCTGTGCAAAAAACCGCTAGTGGAGTACTTTTGCTGTTATGGTGGGGCTAAGTTGTACCAACTCAATAACAACCAACCCCAAGAACAGTACAAGTTTTTAATTGACAGCCGGCAAGTTAAAACAGTGTTTGAAATTGTGGAACAACACAAGGGTTTGTTTTTGGCATACCTCGATAAACCAAAGGCACCCTACATTATTTTGGGGGCAAACCAGTTTTATGCTTGA", "aa": "MIDLLGLDLDGTLLSRTRQINDPTKQALANLIQKKPSLKVMILTGRSLFSTLKYVQELNELCKKPLVEYFCCYGGAKLYQLNNNQPQEQYKFLIDSRQVKTVFEIVEQHKGLFLAYLDKPKAPYIILGANQFYA", "aa_hexdigest": "5e427497861c4538a115bcba94e94620", "start_type": "ATG", "rbs_motif": null, "genes": [ "cof" ], "truncated": "3-prime", "pseudogene": { "start": 316167, "stop": 316874, "observations": { "mutation": [ 316569 ], "stop": [ 316569 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75511", "query_cov": 1.0, "subject_cov": 0.47686832740213525, "identity": 1.0, "score": 272.0, "evalue": 2.04e-90, "gene_start": 1, "gene_end": 134, "reference_start": 1, "reference_end": 134, "reference_sequence": "MIDLLGLDLDGTLLSRTRQINDPTKQALANLIQKKPSLKVMILTGRSLFSTLKYVQELNELCKKPLVEYFCCYGGAKLYQLNNNQPQEQYKFLIDSRQVKTVFEIVEQHKGLFLAYLDKPKAPYIILGANQFYAWLIKQFWYKQRCEYFKNDHLTDGILKINVYFACPLRLKKVYQIIKRQFQDTLNVVNFSKHLIEITHKDGNKGYAIEAIAKKQGLSLKRMAVIGDSLNDRSMFEKVQYSFAMSKSPDELKLLATEIGTKTNRFRFSSLVDLITEKIIN" }, "paralog": false, "identity": 0.9957446808510638, "score": 470.0, "evalue": 6.46e-171, "description": "Internal stop codon at 316569. Nonsense mutation around 316569.", "psc": { "uniref90_id": "UniRef90_P75511", "gene": "Cof", "product": "Putative phosphatase MPN_264", "ec_ids": [ "3.1.3.-" ], "uniref50_id": "UniRef50_P47371", "cog_id": "COG0561", "cog_category": "HR", "go_ids": [ "GO:0005829", "GO:0000287", "GO:0016791" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75511", "EC:3.1.3.-", "UniRef:UniRef50_P47371", "COG:COG0561", "COG:HR", "GO:0005829", "GO:0000287", "GO:0016791" ] }, "pscc": { "uniref50_id": "UniRef50_P47371", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47371" ], "product": "Putative phosphatase MG125" } }, "id": "APKECPOBEN_597", "locus": "GCA002090215_00561" }, { "type": "cds", "sequence": "contig_1", "start": 316653, "stop": 317012, "strand": "+", "frame": 3, "gene": "cof", "product": "Putative phosphatase MPN_264", "db_xrefs": [ "COG:COG0561", "COG:HR", "EC:3.1.3.-", "GO:0000287", "GO:0005829", "GO:0016791", "SO:0001217", "UniRef:UniRef50_P47371", "UniRef:UniRef90_P75511" ], "nt": "GTGTACTTCGCGTGTCCTTTAAGGTTGAAGAAGGTGTACCAGATCATTAAGCGTCAGTTTCAAGACACGCTTAACGTTGTGAATTTTTCCAAGCACTTAATTGAAATTACCCACAAGGATGGAAATAAGGGCTATGCAATTGAAGCAATTGCCAAAAAACAAGGACTTTCGTTAAAACGGATGGCGGTGATTGGTGATTCCTTAAATGACCGTTCGATGTTTGAAAAGGTACAGTATTCGTTTGCAATGTCGAAATCACCCGATGAACTCAAACTGTTAGCCACTGAAATTGGCACTAAAACAAACCGCTTTCGTTTCAGTAGTTTAGTTGATTTAATTACCGAAAAAATTATTAACTAA", "aa": "MYFACPLRLKKVYQIIKRQFQDTLNVVNFSKHLIEITHKDGNKGYAIEAIAKKQGLSLKRMAVIGDSLNDRSMFEKVQYSFAMSKSPDELKLLATEIGTKTNRFRFSSLVDLITEKIIN", "aa_hexdigest": "c85c3973da99617a873371b24966f48c", "start_type": "GTG", "rbs_motif": null, "genes": [ "cof" ], "truncated": "5-prime", "pseudogene": { "start": 316353, "stop": 317012, "observations": { "mutation": [ 316569 ], "stop": [ 316569 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75511", "query_cov": 1.0, "subject_cov": 0.4234875444839858, "identity": 0.992, "score": 233.0, "evalue": 3.42e-75, "gene_start": 1, "gene_end": 119, "reference_start": 163, "reference_end": 281, "reference_sequence": "MIDLLGLDLDGTLLSRTRQINDPTKQALANLIQKKPSLKVMILTGRSLFSTLKYVQELNELCKKPLVEYFCCYGGAKLYQLNNNQPQEQYKFLIDSRQVKTVFEIVEQHKGLFLAYLDKPKAPYIILGANQFYAWLIKQFWYKQRCEYFKNDHLTDGILKINVYFACPLRLKKVYQIIKRQFQDTLNVVNFSKHLIEITHKDGNKGYAIEAIAKKQGLSLKRMAVIGDSLNDRSMFEKVQYSFAMSKSPDELKLLATEIGTKTNRFRFSSLVDLITEKIIN" }, "paralog": false, "identity": 0.9954337899543378, "score": 439.0, "evalue": 5.43e-159, "description": "Internal stop codon at 316569. Nonsense mutation around 316569.", "psc": { "uniref90_id": "UniRef90_P75511", "gene": "Cof", "product": "Putative phosphatase MPN_264", "ec_ids": [ "3.1.3.-" ], "uniref50_id": "UniRef50_P47371", "cog_id": "COG0561", "cog_category": "HR", "go_ids": [ "GO:0005829", "GO:0000287", "GO:0016791" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75511", "EC:3.1.3.-", "UniRef:UniRef50_P47371", "COG:COG0561", "COG:HR", "GO:0005829", "GO:0000287", "GO:0016791" ] }, "pscc": { "uniref50_id": "UniRef50_P47371", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47371" ], "product": "Putative phosphatase MG125" } }, "id": "APKECPOBEN_598", "locus": "GCA002090215_00562" }, { "type": "cds", "sequence": "contig_1", "start": 317020, "stop": 318057, "strand": "-", "frame": 1, "gene": "trpS", "product": "tryptophan--tRNA ligase", "db_xrefs": [ "COG:COG0180", "COG:J", "EC:6.1.1.2", "GO:0000166", "GO:0004830", "GO:0005524", "GO:0005829", "GO:0006436", "SO:0001217", "UniRef:UniRef50_P75510", "UniRef:UniRef90_P75510" ], "nt": "ATGAAACGCGCTCTTACCGGGATTCAAGCATCGGGCAAACAGCATTTAGGCAACTATTTGGGGGTAATGCAGTCGTTAATTGAACTCCAAGAGCAATGCCAACTGTTTGTATTTGTCGCTGATTTACACTCAATTACCGTTGATTTTCAACCCCAAGCACTGAAACAAAATAACTTTGATTTGGTACGTACTTTATTAGCGGTTGGTCTAGATCCACAAAAAGCTTGTCTCTTTTTACAAAGCGATCTTTTGGAGCACAGTATGATGGGTTATTTAATGATGGTGCAAAGCAATCTCGGTGAACTGCAACGGATGACGCAGTTCAAGGCCAAAAAAGCGGAACAAACCCGTAACCCAAACGGCACCTTAAACATTCCCACCGGACTGTTAACTTATCCAGCCCTAATGGCGGGAGATATCTTGCTGTACCAACCTGACATTGTGCCCGTTGGTAATGACCAAAAACAACACCTAGAGCTTACTCGTGATTTAGCGCAGCGAATTCAAAAGAAGTTTAAGCTCAAGCTGCGTCTACCCCAGTTTGTCCAAAACAAAGATACCAACCGTATTATGGACTTATTTGACCCAACCAAAAAGATGAGTAAGTCCTCAAAGAACCAAAACGGAGTAATTTACTTGGATGACCCAAAAGAAGTAGTGGTTAAAAAAATTCGTCAAGCTACCACTGACAGCTTTAACAAAATCCGTTTTGCTCCCAAAACGCAGCCAGGGGTGACCAATATGCTCACAATTCTCAAGGCACTTTTAAAAGAGCCTGTCAATCAATCCTTAACCAATCAGTTAGGGAATGATCTAGAAGCTTACTTTAGTACTAAATCTTATTTAGATCTCAAAAACGCACTTACAGAAGCAACCGTTAATCTGCTAGTTAACATTCAGAGAAAACGGGAACAGATTAGTCGTGAACAGGTCTTTAACTGCCTCCAGGCGGGTAAAAATCAAGCCCAAGCAACGGCCAGAACAACACTAGCACTTTTCTATGATGGCTTTGGACTAGGAAGCCAAAACATCAAGTAA", "aa": "MKRALTGIQASGKQHLGNYLGVMQSLIELQEQCQLFVFVADLHSITVDFQPQALKQNNFDLVRTLLAVGLDPQKACLFLQSDLLEHSMMGYLMMVQSNLGELQRMTQFKAKKAEQTRNPNGTLNIPTGLLTYPALMAGDILLYQPDIVPVGNDQKQHLELTRDLAQRIQKKFKLKLRLPQFVQNKDTNRIMDLFDPTKKMSKSSKNQNGVIYLDDPKEVVVKKIRQATTDSFNKIRFAPKTQPGVTNMLTILKALLKEPVNQSLTNQLGNDLEAYFSTKSYLDLKNALTEATVNLLVNIQRKREQISREQVFNCLQAGKNQAQATARTTLALFYDGFGLGSQNIK", "aa_hexdigest": "573988a65f913ed792160f486f7fef73", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75510", "query_cov": 1.0, "subject_cov": 0.9913793103448276, "identity": 0.997, "score": 660.0, "evalue": 3.62e-239, "valid": true, "gene": "trpS", "product": "tryptophan--tRNA ligase", "ec_ids": [ "6.1.1.2" ], "uniref50_id": "UniRef50_P75510", "cog_id": "COG0180", "cog_category": "J", "go_ids": [ "GO:0000166", "GO:0004830", "GO:0005524", "GO:0005829", "GO:0006436" ] }, "pscc": { "uniref50_id": "UniRef50_P75510", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75510" ], "product": "Tryptophan--tRNA ligase" }, "genes": [ "trpS" ], "id": "APKECPOBEN_599", "locus": "GCA002090215_00563" }, { "type": "cds", "sequence": "contig_1", "start": 318117, "stop": 318554, "strand": "+", "frame": 3, "gene": "arsC", "product": "putative MG127-like protein", "db_xrefs": [ "COG:COG1393", "COG:P", "GO:0016491", "KEGG:K16509", "RefSeq:WP_010874623.1", "SO:0001217", "UniParc:UPI0000139303", "UniRef:UniRef100_P75509", "UniRef:UniRef50_P75509", "UniRef:UniRef90_P75509" ], "nt": "ATGCTTAAGAAAAAAGTTAATAATGATGCTGGTAAGACGTTTATCTTAATTTCATCATCTTGCTCGTCTTGCCAAAAGGCAATCGAATACTTTAAGGAAAATAATTTGAGTTATACGATCGAAAACTTTTACAAAAAACCGATAAGTGACAAGCGCTTTCGTGACATTTTAAGTTTGAGTGAAGATGGCACCGAAAGTCTGTTTTCCAAGCGTGCTGACCAAATTCGTAGTAACACTAATCAATCAGTGGAAGATTTGACCATTCCTGAATTGATTAAGTTAATTCGCGAACGTCCTTCAATTTTGCGTCGACCAATTATTATCCAGTACAACTCTTCGGGGATTCCCAAGCGGATGCGCATTGGTTATAACGCCGCTGAAATTAAGGTATTTGAACGCAGCTTAATGGAACCTAAAGTGCGTACAGTAAAGAAGTAA", "aa": "MLKKKVNNDAGKTFILISSSCSSCQKAIEYFKENNLSYTIENFYKKPISDKRFRDILSLSEDGTESLFSKRADQIRSNTNQSVEDLTIPELIKLIRERPSILRRPIIIQYNSSGIPKRMRIGYNAAEIKVFERSLMEPKVRTVKK", "aa_hexdigest": "f928a8bde45f22565b6347623ee5e9b0", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000139303", "ncbi_nrp_id": "WP_010874623.1", "uniref100_id": "UniRef100_P75509", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139303", "RefSeq:WP_010874623.1", "UniRef:UniRef100_P75509" ] }, "ips": { "uniref100_id": "UniRef100_P75509", "uniref90_id": "UniRef90_P75509" }, "psc": { "uniref90_id": "UniRef90_P75509", "gene": "ArsC", "product": "Uncharacterized protein MG127 homolog", "uniref50_id": "UniRef50_P75509", "cog_id": "COG1393", "cog_category": "P", "kegg_orthology_id": "K16509", "go_ids": [ "GO:0016491" ] }, "pscc": { "uniref50_id": "UniRef50_P75509", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75509" ], "product": "Uncharacterized protein MG127 homolog" }, "genes": [ "arsC" ], "id": "APKECPOBEN_600", "locus": "GCA002090215_00564" }, { "type": "cds", "sequence": "contig_1", "start": 318554, "stop": 319333, "strand": "+", "frame": 2, "gene": "nadK", "product": "NAD(+) kinase", "db_xrefs": [ "COG:COG0061", "COG:H", "EC:2.7.1.23", "GO:0003951", "GO:0005524", "GO:0005737", "GO:0006741", "GO:0019674", "GO:0046872", "GO:0051287", "KEGG:K00858", "RefSeq:WP_010874624.1", "SO:0001217", "UniParc:UPI00001320B9", "UniRef:UniRef100_P75508", "UniRef:UniRef50_P75508", "UniRef:UniRef90_P75508" ], "nt": "ATGAAGTACAAGATTTTTGCATCAACAACACCACAAACCGAGCCAGTTTTGCAAAAGTTAAAGCAGGTGTTGAAAGGTTGTGAGGCAGTTGAAAAGGGCTTTGATTATCTCTTTGTATTAGGTGGTGATGGTTTCTTTGTTTCTACTGTGGCCAACTATAACTGCCACAATTGTAGGGTAGTAGGGATTAACACTGGTCATCTAGGTTTTTACACATCGTTTAATGAAAAGGATTTGGATGATAATTTCCTCCAAAAGCTCCAGCAATGTCACTTTCAACGGATTAGTTTGTTGGAAGTTAGTGTCAATGGCCAGCAGCACTTGGTTTTGAATGAACTGGCAGTTTACACCAACACAGCTTACCCAATTAACATCTTTATTGATGGTGAAGCGTGGGAGTTTTACCGCGGTTCAGGTTTATTAATCGGTCCGCGTACTGGTTCGACTGCACTGGCTAAATCTGCTAAAGGGGCGGTGATTTTCCCTGGCATTGATGTGCTGCAAATTATTGAAATGAATCCACTTTTGCACCCCAATCAAGTTACTATCCAATCACCAATTATTTTGCCCAAAGAAACGCAAGTGGAATTTGTGGTCAAAAAAGCCTTTAACCCACAACAGTTTCCCACCTTTTACTGTGACGGGAGAAAGTTGGAATTGCCAAACGCTGACACAACTTTAGCGCTTAAACTAGTGCAATCCACACCGATGTTTAACATTTCACTCAAAACACAGGACTTTATCAATAAACTTAAGTCCACTTTTATTAAACAAAGCTAA", "aa": "MKYKIFASTTPQTEPVLQKLKQVLKGCEAVEKGFDYLFVLGGDGFFVSTVANYNCHNCRVVGINTGHLGFYTSFNEKDLDDNFLQKLQQCHFQRISLLEVSVNGQQHLVLNELAVYTNTAYPINIFIDGEAWEFYRGSGLLIGPRTGSTALAKSAKGAVIFPGIDVLQIIEMNPLLHPNQVTIQSPIILPKETQVEFVVKKAFNPQQFPTFYCDGRKLELPNADTTLALKLVQSTPMFNISLKTQDFINKLKSTFIKQS", "aa_hexdigest": "2a76f8b4329ae23c917b427157968987", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001320B9", "ncbi_nrp_id": "WP_010874624.1", "uniref100_id": "UniRef100_P75508", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001320B9", "RefSeq:WP_010874624.1", "UniRef:UniRef100_P75508" ] }, "ips": { "uniref100_id": "UniRef100_P75508", "uniref90_id": "UniRef90_P75508" }, "psc": { "uniref90_id": "UniRef90_P75508", "gene": "nadK", "product": "NAD(+) kinase", "ec_ids": [ "2.7.1.23" ], "uniref50_id": "UniRef50_P75508", "cog_id": "COG0061", "cog_category": "H", "kegg_orthology_id": "K00858", "go_ids": [ "GO:0005737", "GO:0005524", "GO:0046872", "GO:0051287", "GO:0003951", "GO:0019674", "GO:0006741" ] }, "pscc": { "uniref50_id": "UniRef50_P75508", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75508" ], "product": "NAD kinase" }, "genes": [ "nadK" ], "id": "APKECPOBEN_601", "locus": "GCA002090215_00565" }, { "type": "cds", "sequence": "contig_1", "start": 319360, "stop": 319686, "strand": "+", "frame": 1, "gene": "ptsG2", "product": "Putative phosphotransferase enzyme IIB component MPN_268", "db_xrefs": [ "COG:COG1264", "COG:G", "EC:2.7.1.-", "GO:0008982", "GO:0009401", "GO:0016020", "SO:0001217", "UniRef:UniRef50_P75507", "UniRef:UniRef90_P75507" ], "nt": "GTGTTGAGCTTTGGCTTACTTTACCTTTATTTGGTAAAACGTGCTAAGAGGGCCGCGCTACAATTAAACAACAAACTAGTCGAATCACACACAATTCCTTTTGCTGTACGTGACTTTATTGCCGCTTGCGGGGGTAGAACTAACTTTGTTAGTTTACGCACCTCACCCACCCAATTAATTGTTAGTTTTGCCAAGCCAGAGTTAGTTCAAATTGCCGCTTTGCAAAAGCTTGGAATTAAAGGGATTAATAAGAGTCAGAACCAGTACCGCTTTGTATTGGGCAATTTTGTCAATCAATTAAAACAGCAAATTGAAAATGAGCGCTAA", "aa": "MLSFGLLYLYLVKRAKRAALQLNNKLVESHTIPFAVRDFIAACGGRTNFVSLRTSPTQLIVSFAKPELVQIAALQKLGIKGINKSQNQYRFVLGNFVNQLKQQIENER", "aa_hexdigest": "13d04c6ff2e45ac7835b0b3ce2bab351", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75507", "query_cov": 0.9907407407407407, "subject_cov": 0.9145299145299145, "identity": 1.0, "score": 204.0, "evalue": 1.39e-66, "valid": true, "gene": "PtsG2", "product": "Putative phosphotransferase enzyme IIB component MPN_268", "ec_ids": [ "2.7.1.-" ], "uniref50_id": "UniRef50_P75507", "cog_id": "COG1264", "cog_category": "G", "go_ids": [ "GO:0016020", "GO:0008982", "GO:0009401" ] }, "pscc": { "uniref50_id": "UniRef50_P75507", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75507" ], "product": "Putative phosphotransferase enzyme IIB component MPN_268" }, "genes": [ "ptsG2" ], "id": "APKECPOBEN_602", "locus": "GCA002090215_00566" }, { "type": "cds", "sequence": "contig_1", "start": 319676, "stop": 320989, "strand": "+", "frame": 2, "gene": "rny", "product": "Ribonuclease Y", "db_xrefs": [ "COG:COG1418", "COG:JR", "EC:3.1.-.-", "GO:0003723", "GO:0004521", "GO:0005886", "GO:0006402", "SO:0001217", "UniRef:UniRef50_P47376", "UniRef:UniRef90_P75506" ], "nt": "ATGAGCGCTAAGTTAACGTTAGAAAGCATTGCAAAAACATTTGCCGAAACATCGATCTTTGCGATCTTGTTTTTAATTATTGTCATCCTCAATTTAGGTTTACTTGTTTTTTTAGCGTATCAGTACCGTGTTTACAAAAAGAAGCAAAGAGCAAACCTCACCAAGCAGACTTATAACAATGACTATGGCCAAATTGTTAATTTAAAACAGCAAAACGGTGCCAAGATAAAGGAGCTTGCTAACTTAAAAGATCAGTTAAGTGAATTAGGCCAAAAATTTAACACCACACTGAGTGAAATCATTAACAAACCACTTGTCAATGTGATTGATGAATACTTGGATGAACAGTTTAAACAAGCTGCTAATTTTCGTGAAGCAGAACTTAATGCCGTTTTAGATAGTAATGACCAGAAAACAGTGTTCCATAAGCGTTTGTTCAATAAATTTCACTTTGGTGTCGACAAGCTAGCTAATATTAATGTCAAAAATCCGCTCAATTTATGCTGGGTTGACAGCGCTAGTTTTACCGTGATTGAAAGTGATTTCCGCAAACTTAATGGTGTTGGCGGGATTAATAAAAAGTTGTTAATTGAAAAGTTAAGGATTGAAGATATTATCTTTACCAATATCGATAAGAAGTACTATGAGGTACAGATACTATCTGACTCGCCAGTAAAGGTACAAAAAACAGTGTTAACTATTCGCAACATCTTGATTAATGATTACGTGGACAATGAAAAAATTGAGTCATATGCTAGGGAAGCTAACGGTTACTTCAATGACCACTGTAAACTGATTGGTAAGCAGGTGTTAGAACGACTCAATATCTTTGAAATTAGCCCTAAACTACACAAGTTTTTTGGTTTATTAGCGTTTCGTTATTCTTTCGGTCAAAACGTTTTATCACACTGTCTGGAAACTGGTTTTCTCACCGCTTACCTAGCGCTTTTAGTTAACTTTAAACCTGATGTTGCATTAAAGTGCGGTTTGTATCATGATATTGGTAAAGCGGATGATGAAAACGGGAAGAAGAACCACACGGTTACAGGTGCCAAGATCGGTGATGAATTTTACTTTGAAAACGATGTCAAATACACAATTGCCAACCACCATAATAAAAATGTCGATAACGTATACTGTCGTTTAACTCAAATTGGCGATAAGCTTTCAGCAGGGCGTTTGGGTGCGCGCTCTGACAGTTCGGTTTTATTTAGCCAACTCAAACAAGAATTAAAGCAAATTGTGGAAGAAACACTAGCACAATTTAAAACAACCATCCTTTTAGGACAAAGTGGACGCCGATTAGTAATTTGA", "aa": "MSAKLTLESIAKTFAETSIFAILFLIIVILNLGLLVFLAYQYRVYKKKQRANLTKQTYNNDYGQIVNLKQQNGAKIKELANLKDQLSELGQKFNTTLSEIINKPLVNVIDEYLDEQFKQAANFREAELNAVLDSNDQKTVFHKRLFNKFHFGVDKLANINVKNPLNLCWVDSASFTVIESDFRKLNGVGGINKKLLIEKLRIEDIIFTNIDKKYYEVQILSDSPVKVQKTVLTIRNILINDYVDNEKIESYAREANGYFNDHCKLIGKQVLERLNIFEISPKLHKFFGLLAFRYSFGQNVLSHCLETGFLTAYLALLVNFKPDVALKCGLYHDIGKADDENGKKNHTVTGAKIGDEFYFENDVKYTIANHHNKNVDNVYCRLTQIGDKLSAGRLGARSDSSVLFSQLKQELKQIVEETLAQFKTTILLGQSGRRLVI", "aa_hexdigest": "8bbbc23b8e6928f2aa5cf862e9ebf2ae", "start_type": "ATG", "rbs_motif": "AAAA", "psc": { "uniref90_id": "UniRef90_P75506", "query_cov": 1.0091533180778032, "subject_cov": 0.8873239436619719, "identity": 0.98, "score": 829.0, "evalue": 7.11e-302, "valid": true, "gene": "rny", "product": "Ribonuclease Y", "ec_ids": [ "3.1.-.-" ], "uniref50_id": "UniRef50_P47376", "cog_id": "COG1418", "cog_category": "JR", "go_ids": [ "GO:0005886", "GO:0003723", "GO:0004521", "GO:0006402" ] }, "pscc": { "uniref50_id": "UniRef50_P47376", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47376" ], "product": "Ribonuclease Y" }, "genes": [ "rny" ], "id": "APKECPOBEN_603", "locus": "GCA002090215_00567" }, { "type": "cds", "sequence": "contig_1", "start": 320990, "stop": 321157, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAAACCAACCAGCACAATAACATTATTGACAACCAACAGTTAACTGATTTGGCTACTACCATTAAAAGCAAAATCGTGCAAAACAACATTACTAATCGCTTTCCAATTAAGGTAGTATTACGCTATAACTTCGAACACAGCTTTGATACAAAGGACAAGAACTAG", "aa": "METNQHNNIIDNQQLTDLATTIKSKIVQNNITNRFPIKVVLRYNFEHSFDTKDKN", "aa_hexdigest": "a8d39b1aa755cf66f792235a554d7531", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6504.217700000001, "isoelectric_point": 8.140245628356933 }, "id": "APKECPOBEN_604", "locus": "GCA002090215_00568" }, { "type": "cds", "sequence": "contig_1", "start": 321160, "stop": 321447, "strand": "+", "frame": 1, "gene": null, "product": "putative MG131-like protein", "db_xrefs": [ "GO:0005886", "RefSeq:WP_010874627.1", "SO:0001217", "UniParc:UPI000013931E", "UniRef:UniRef100_Q9EXD1", "UniRef:UniRef50_Q9EXD1", "UniRef:UniRef90_Q9EXD1" ], "nt": "ATGCAATACACCGTTTTAATTCCACTGTTTATTTTTATAGGAGCCATGGTGTTGTTTGGTTTTAGCTTTCAGAAAAAGCAACCACAACGCCGCATCGTTCAAATTCTGTTCTTAGCTTACTGTGTGGATTTTTTAGCACTAATTATTGCAGTGATGATGCTCACATTCTTAAGTTATGATGACTTAATGCTGGGCGTTTTAATTCCAGTATTAATCCTTTCAATCATTATGTTCTTTGTGATGATTATTGCCCACTATCCACTAATGAAACGCTTGTTTGGACATTAG", "aa": "MQYTVLIPLFIFIGAMVLFGFSFQKKQPQRRIVQILFLAYCVDFLALIIAVMMLTFLSYDDLMLGVLIPVLILSIIMFFVMIIAHYPLMKRLFGH", "aa_hexdigest": "c68edcf8234a323dcf7d6b19cf7a548a", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013931E", "ncbi_nrp_id": "WP_010874627.1", "uniref100_id": "UniRef100_Q9EXD1", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013931E", "RefSeq:WP_010874627.1", "UniRef:UniRef100_Q9EXD1" ] }, "ips": { "uniref100_id": "UniRef100_Q9EXD1", "uniref90_id": "UniRef90_Q9EXD1" }, "psc": { "uniref90_id": "UniRef90_Q9EXD1", "product": "Uncharacterized protein MG131 homolog", "uniref50_id": "UniRef50_Q9EXD1", "go_ids": [ "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_Q9EXD1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9EXD1" ], "product": "Uncharacterized protein MG131 homolog" }, "genes": [], "id": "APKECPOBEN_605", "locus": "GCA002090215_00569" }, { "type": "cds", "sequence": "contig_1", "start": 321709, "stop": 321957, "strand": "+", "frame": 1, "gene": null, "product": "putative lipoprotein MPN_467", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P75316", "UniRef:UniRef90_P75316" ], "nt": "ATGCGTAAAAAGAAGTTTTTGTCAAGGTTTTCTTTTAGTTCTCTATTTTTATTATGTGGAACATTGTTAAGTGCTTGCACTGGTATCCAAGCTGATCTTAGAAATCTTATTAAAGAAACCACTGGTAAAGACATTGATTTGTCTAAAGCAATTAAAACTAAAGAAGGCAAAAAGAACATAATAGCTTCTTTAAAAAAATCTTACGAAGTTAATCCGAAAGATACAACAAAATTGTTGTTAGACGCCTGA", "aa": "MRKKKFLSRFSFSSLFLLCGTLLSACTGIQADLRNLIKETTGKDIDLSKAIKTKEGKKNIIASLKKSYEVNPKDTTKLLLDA", "aa_hexdigest": "65824dd032ed54499f73711e7427fdc8", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75316", "query_cov": 1.0, "subject_cov": 0.8118811881188119, "identity": 0.951, "score": 148.0, "evalue": 5.28e-45, "valid": true, "product": "Uncharacterized lipoprotein MPN_467", "uniref50_id": "UniRef50_P75316", "go_ids": [ "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75316", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75316" ], "product": "Uncharacterized lipoprotein MPN_467" }, "genes": [], "id": "APKECPOBEN_606", "locus": "GCA002090215_00570" }, { "type": "cds", "sequence": "contig_1", "start": 322045, "stop": 322134, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAGCGAAAGGTTGATCATTTTCAAATGACATACCAAAGTTTTAAGGATTTGTTAGTTGAGGCTAGGCTGAGCTATACCTTTAATTGA", "aa": "MERKVDHFQMTYQSFKDLLVEARLSYTFN", "aa_hexdigest": "e34c401a42d6c8923a0331cea5fd4f57", "start_type": "ATG", "rbs_motif": "AACAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3598.0693, "isoelectric_point": 6.518261528015137 }, "id": "APKECPOBEN_607", "locus": "GCA002090215_00571" }, { "type": "cds", "sequence": "contig_1", "start": 322154, "stop": 322306, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATTTCACTGCTAAGAGAGGTGACAAGCATTATTTTTATCTTTTCCTTAAAATTAAATCTGATCCAAAAAAACAATTTTCAGCAAAAAAATTTCTTACTGAAGGAGAAGCGTTTACTGATCAAGAAGGAAAACAAACTACAAGAAATTTAG", "aa": "MISLLREVTSIIFIFSLKLNLIQKNNFQQKNFLLKEKRLLIKKENKLQEI", "aa_hexdigest": "fff8c858299082528508dd4a4a4b764c", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6060.288099999998, "isoelectric_point": 10.214515876770019 }, "id": "APKECPOBEN_608", "locus": "GCA002090215_00572" }, { "type": "cds", "sequence": "contig_1", "start": 323507, "stop": 323734, "strand": "-", "frame": 3, "gene": null, "product": "restriction endonuclease subunit S", "db_xrefs": [ "EC:3.1.21.3", "KEGG:K01154", "SO:0001217", "UniRef:UniRef50_A0AB38W825", "UniRef:UniRef90_UPI0035BBF72E" ], "nt": "TTGCAAACATCTAAGTTTGTTAATTATGCTTGCCCTATTCCTAAATTAGGAATAAGTAAATTAGCAGAAATTGAACTTACTTTCCCACCTCTAGAAATTCAAGAAAAGATTGCCGATATCCTCTTTGCTTTTGAAAAAAATGTAACGATCTTACCGAAAGTATTCCCACAGAAATTGAAATGCGTAAAAAAGCAATTGGATTATTACCAAAACTATTTATTCGATTGA", "aa": "MQTSKFVNYACPIPKLGISKLAEIELTFPPLEIQEKIADILFAFEKNVTILPKVFPQKLKCVKKQLDYYQNYLFD", "aa_hexdigest": "e0bd4c6eb86df3a47a0374d69b571bd9", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 323462, "stop": 323926, "observations": { "mutation": [ 323509, 323866 ], "stop": [ 323509, 323866 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0035BBF72E", "query_cov": 1.0, "subject_cov": 0.487012987012987, "identity": 0.987, "score": 154.0, "evalue": 7.39e-47, "gene_start": 1, "gene_end": 75, "reference_start": 65, "reference_end": 139, "reference_sequence": "MNDGEVGKIKDCDRDGEYVTWTTGGSYAGAVFYRKGQFNARQDCGVLKVKNEEINPKFLAYALRLQTSKFVNYACPIPKLGISKLAEIELTFPPLEIQEKIADILFAFEKNVTILPKVFPQKLKCVKKQLDYYQNYLFDWVQKQKEGLNSTTLN" }, "paralog": false, "identity": 0.9805194805194806, "score": 306.0, "evalue": 5.09e-109, "description": "Internal stop codon at 323509, 323866. Nonsense mutation around 323509, 323866.", "psc": { "uniref90_id": "UniRef90_UPI0035BBF72E", "product": "restriction endonuclease subunit S", "ec_ids": [ "3.1.21.3" ], "uniref50_id": "UniRef50_A0AB38W825", "kegg_orthology_id": "K01154", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0035BBF72E", "EC:3.1.21.3", "UniRef:UniRef50_A0AB38W825", "KEGG:K01154" ] }, "pscc": { "uniref50_id": "UniRef50_A0AB38W825", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W825" ], "product": "Restriction-modification enzyme subunit S1B" } }, "id": "APKECPOBEN_609", "locus": "GCA002090215_00573" }, { "type": "cds", "sequence": "contig_1", "start": 324170, "stop": 324373, "strand": "+", "frame": 2, "gene": null, "product": "putative protein MPN_272", "db_xrefs": [ "GO:0016020", "SO:0001217", "UniRef:UniRef50_Q9EXD2", "UniRef:UniRef90_Q9EXD2" ], "nt": "ATGAACCGTCCTACTCCCAATTTTGAAGCAATCGATAAGAAGATTAGTGCCTTTGTCACTAACCACGATAACTTATTGGATAAGTTACTCAAACAACAAACCGAATTGTTAACTAGTGAAATTACGACTAATTTTGAGGTAACCCAACAAATCCAGGAAGAAGTAGCCAAAAAGACGAAGCAACACTCTAAAAACTACAAGTGA", "aa": "MNRPTPNFEAIDKKISAFVTNHDNLLDKLLKQQTELLTSEITTNFEVTQQIQEEVAKKTKQHSKNYK", "aa_hexdigest": "669edc04f61a0ea699ba98706a6e0463", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 324170, "stop": 324451, "observations": { "mutation": [ 324371 ], "stop": [ 324371 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9EXD2", "query_cov": 1.0, "subject_cov": 0.7204301075268817, "identity": 1.0, "score": 128.0, "evalue": 1.89e-37, "gene_start": 1, "gene_end": 67, "reference_start": 1, "reference_end": 67, "reference_sequence": "MNRPTPNFEAIDKKISAFVTNHDNLLDKLLKQQTELLTSEITTNFEVTQQIQEEVAKKTKQHSKNYKWLVTVVLANGVVSLFLLGGLIYLFSK" }, "paralog": false, "identity": 0.989247311827957, "score": 179.0, "evalue": 9.32e-60, "description": "Internal stop codon at 324371. Nonsense mutation around 324371.", "psc": { "uniref90_id": "UniRef90_Q9EXD2", "product": "Uncharacterized protein MPN_272", "uniref50_id": "UniRef50_Q9EXD2", "go_ids": [ "GO:0016020" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9EXD2", "UniRef:UniRef50_Q9EXD2", "GO:0016020" ] }, "pscc": { "uniref50_id": "UniRef50_Q9EXD2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9EXD2" ], "product": "Uncharacterized protein MPN_272" } }, "id": "APKECPOBEN_610", "locus": "GCA002090215_00574" }, { "type": "cds", "sequence": "contig_1", "start": 324428, "stop": 324862, "strand": "-", "frame": 3, "gene": "hinT", "product": "putative HIT-like protein", "db_xrefs": [ "COG:COG0537", "COG:FR", "GO:0003824", "GO:0009117", "RefSeq:WP_010874630.1", "SO:0001217", "UniParc:UPI000013B281", "UniRef:UniRef100_P75504", "UniRef:UniRef50_P47378", "UniRef:UniRef90_P75504" ], "nt": "ATGGTGCAAAAACAAAGTATGGCAAACAATAACTGTATCTTCTGTGGAATTGTAGAGGGGAATGTGAAATCCTTTAAGGTTGGTGAAAACGAACACGCTTTTGCCTTTTTAGATGCCTTTCCAGTTGCTGATGGTCATACGCTTGTAATTCCCAAAAAACACGCCGTTAACTATTCCTCGACTGATGATGAGAGTTTGAAGGCGGTTAGTTTACTCGCCAAAGAAATGGCTTTAAAGCTACAACAACGGCTCCAACCAGCTGGATTAAACTATGTTGTCAATGAAGGGGCTAAGGCAGGGCAGGAAGTATTTCACTACCACATGCATGTAGTACCAAAATACGAAACTGGTTTAGGCTTTTGTTACAACGTTAGAAAGACGAACAACCGCTCAATTGAAGCTAATTGGGAACTACTTACTAAAGAGGTAGATTAA", "aa": "MVQKQSMANNNCIFCGIVEGNVKSFKVGENEHAFAFLDAFPVADGHTLVIPKKHAVNYSSTDDESLKAVSLLAKEMALKLQQRLQPAGLNYVVNEGAKAGQEVFHYHMHVVPKYETGLGFCYNVRKTNNRSIEANWELLTKEVD", "aa_hexdigest": "f20e897af49fed98213163bc282f3ff9", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013B281", "ncbi_nrp_id": "WP_010874630.1", "uniref100_id": "UniRef100_P75504", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013B281", "RefSeq:WP_010874630.1", "UniRef:UniRef100_P75504" ] }, "ips": { "uniref100_id": "UniRef100_P75504", "uniref90_id": "UniRef90_P75504" }, "psc": { "uniref90_id": "UniRef90_P75504", "gene": "HinT", "product": "Uncharacterized 16.1 kDa HIT-like protein", "uniref50_id": "UniRef50_P47378", "cog_id": "COG0537", "cog_category": "FR", "go_ids": [ "GO:0003824", "GO:0009117" ] }, "pscc": { "uniref50_id": "UniRef50_P47378", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47378" ], "product": "Uncharacterized HIT-like protein MG132" }, "genes": [ "hinT" ], "id": "APKECPOBEN_611", "locus": "GCA002090215_00575" }, { "type": "cds", "sequence": "contig_1", "start": 324930, "stop": 325109, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCGCGAGCCGCTACATGAATTGGGTAAGTTTTTACTCAAGGCAGGGGTGTACCCAAGCCTTTATGCCTTTTATTTACAAACAGTACCTTTTCTAAAGATATGGGACAACGGGCATGATTCGTACTCGGTCTATGGTTTCTTCTCCCAAACCAAATATAACAGCTATGTTTGGTTTTGA", "aa": "MREPLHELGKFLLKAGVYPSLYAFYLQTVPFLKIWDNGHDSYSVYGFFSQTKYNSYVWF", "aa_hexdigest": "6600a0c7effaa00232f4fb117449c3e0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7063.991900000001, "isoelectric_point": 8.003379249572756 }, "id": "APKECPOBEN_612", "locus": "GCA002090215_00576" }, { "type": "cds", "sequence": "contig_1", "start": 325194, "stop": 325631, "strand": "-", "frame": 2, "gene": null, "product": "putative MG133-like protein", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P47379", "UniRef:UniRef90_P75503" ], "nt": "ATGAAAAAAACAATAGGACTCGCTTACCGTTTTTTCTATCTTAACAATAATTGCGACTTTTATTTACTTTTTCTCGCGCCGTTTTCCCTGTTTAACCTTGGGGTAATGGTAGCTAGTGCTGTTATCAGTGTGGTTTATAACAACCAACCACAATTAATCTGGTTTACTAACTTTGATACCTTTACCTACCAGAGCAACACAATTGCCGCTGTTTGTGTCTTAATGTACCTCTGTAAACGACGCTGTAAGCTGTTTGACAATAATGCGTTGTTTTTAAGTGCTGCAGGTTACTTGGTCTTTACCGTCATCTTTTTTAACCTGTATGTACTGTCACGTGTTACTGGATTTGTTAATGTAGAAGAACACGTTAAGGGTTGGTTTTCGACTATTACGAGTGAAATGCCATACAGTTTTAGTGGTAATCCTTTAACTGATTGA", "aa": "MKKTIGLAYRFFYLNNNCDFYLLFLAPFSLFNLGVMVASAVISVVYNNQPQLIWFTNFDTFTYQSNTIAAVCVLMYLCKRRCKLFDNNALFLSAAGYLVFTVIFFNLYVLSRVTGFVNVEEHVKGWFSTITSEMPYSFSGNPLTD", "aa_hexdigest": "169616fd36fea1a5825ef43ee09f50b5", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 324891, "stop": 325631, "observations": { "mutation": [ 324932, 325133, 325196 ], "stop": [ 324932, 325133, 325196 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75503", "query_cov": 1.0, "subject_cov": 0.5451127819548872, "identity": 1.0, "score": 293.0, "evalue": 5.94e-99, "gene_start": 1, "gene_end": 145, "reference_start": 1, "reference_end": 145, "reference_sequence": "MKKTIGLAYRFFYLNNNCDFYLLFLAPFSLFNLGVMVASAVISVVYNNQPQLIWFTNFDTFTYQSNTIAAVCVLMYLCKRRCKLFDNNALFLSAAGYLVFTVIFFNLYVLSRVTGFVNVEEHVKGWFSTITSEMPYSFSGNPLTDWISFAQLFLHVIYPASFFGFIWIFFKTYKMREPLHELGKFLLKAGVYPSLYAFYLQTVPFLKIWDNGHDSYSVYGFFSQTKYNSYVWFWSIPIFASMFLILWGLFVINNRYYGAKTKYGKQ" }, "paralog": false, "identity": 0.9878048780487805, "score": 497.0, "evalue": 1.79e-181, "description": "Internal stop codon at 324932, 325133, 325196. Nonsense mutation around 324932, 325133, 325196.", "psc": { "uniref90_id": "UniRef90_P75503", "product": "Uncharacterized protein MG133 homolog", "uniref50_id": "UniRef50_P47379", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75503", "UniRef:UniRef50_P47379", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47379", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47379" ], "product": "Uncharacterized protein MG133" } }, "id": "APKECPOBEN_613", "locus": "GCA002090215_00577" }, { "type": "cds", "sequence": "contig_1", "start": 325653, "stop": 325955, "strand": "+", "frame": 3, "gene": "ybaB", "product": "YbaB/EbfC family nucleoid-associated protein", "db_xrefs": [ "COG:COG0718", "COG:K", "GO:0003677", "KEGG:K09747", "RefSeq:WP_010874632.1", "SO:0001217", "UniParc:UPI000013932F", "UniRef:UniRef100_P75502", "UniRef:UniRef50_P47380", "UniRef:UniRef90_P75502" ], "nt": "ATGAGTTTTAAAAAGATTACCGAAATGATGCGCCAAGCTGAACGACAAAGCAAGCAAAAAGCGCTTGATTTCGAGCAAAAATTGTTTGAGTACAGCTATAAGAATGCTGCCATCAAGATTATCATTTTTGGTAATCTCACCATTAAGTCTATCACAATCGATCCCGCATTAATTGACCCAGAAGACAAGGTCACTTTAGAGGAGATGATTACAGAAGCTGTCAATGAGGCTGTGGGTGATGTTAAGGCCAAGTACGACCAGTTAATGGAAGAGGCTATGCCACAGATGCCTGGGCTTTTTTAA", "aa": "MSFKKITEMMRQAERQSKQKALDFEQKLFEYSYKNAAIKIIIFGNLTIKSITIDPALIDPEDKVTLEEMITEAVNEAVGDVKAKYDQLMEEAMPQMPGLF", "aa_hexdigest": "85a8ee1bfe713a32c945844c5f7bc40e", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013932F", "ncbi_nrp_id": "WP_010874632.1", "uniref100_id": "UniRef100_P75502", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013932F", "RefSeq:WP_010874632.1", "UniRef:UniRef100_P75502" ] }, "ips": { "uniref100_id": "UniRef100_P75502", "uniref90_id": "UniRef90_P75502" }, "psc": { "uniref90_id": "UniRef90_P75502", "gene": "YbaB", "product": "YbaB/EbfC family nucleoid-associated protein", "uniref50_id": "UniRef50_P47380", "cog_id": "COG0718", "cog_category": "K", "kegg_orthology_id": "K09747", "go_ids": [ "GO:0003677" ] }, "pscc": { "uniref50_id": "UniRef50_P47380", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47380" ], "product": "Uncharacterized protein MG134" }, "genes": [ "ybaB" ], "id": "APKECPOBEN_614", "locus": "GCA002090215_00578" }, { "type": "cds", "sequence": "contig_1", "start": 325927, "stop": 326112, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTCCTCAAGCTAGATCCAAAAAATGCTGATTTAATCTTTGGAGTTAGCTTTGGGTTGAGCTATTTCGCCATTGTATTGTTGACAGTTCTCGTCAACATTCCAACCTTTATACAGCTCACTAAAGATAAACAAATGGTTAAGCTAGCACTGCAGCTTAAAAAAGCCCAGGCATCTGTGGCATAG", "aa": "MFLKLDPKNADLIFGVSFGLSYFAIVLLTVLVNIPTFIQLTKDKQMVKLALQLKKAQASVA", "aa_hexdigest": "70b638538c507ceb45fa1c599637e4d5", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6754.133800000002, "isoelectric_point": 9.778516197204588 }, "id": "APKECPOBEN_615", "locus": "GCA002090215_00579" }, { "type": "cds", "sequence": "contig_1", "start": 326140, "stop": 326238, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTTGCGCTAGCATATCTCAGTTATGTGGCCTTGATGGTTAACATCTTTAAGGGACAAATTACCGGTAATACTACCGGCATTTTGGCTGAAGATTGA", "aa": "MFALAYLSYVALMVNIFKGQITGNTTGILAED", "aa_hexdigest": "8ae28675fb334f1ce0469fc7933e585d", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3466.0291000000007, "isoelectric_point": 4.667413520812987 }, "id": "APKECPOBEN_616", "locus": "GCA002090215_00580" }, { "type": "cds", "sequence": "contig_1", "start": 326278, "stop": 326784, "strand": "-", "frame": 1, "gene": null, "product": "putative MG135-like protein", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P47381", "UniRef:UniRef90_P75501" ], "nt": "ATGCAGTCGCTTAACTATCTTGTAGTCATCCTAACAGTAGCCGGGGTGCTAGTTATCTTGGGATTTACCCCATTAATTCGCAAATTAAAAATCCAGTTTTATTGTTTACAAGTTTTTGCAGCTATTCTCTTTCTCTATGTCTTTTTCGGGCGGCAAATTATCTATATTTTTCCCGATATTTACGGCACTGCAGCCAAGGCTAAGAATGCTGTTGCAAATGTTCCTTTAGATTCACTAAGGTTGAGCAGGATCTTTTTGTTAGATCTCTGCCCCTTCTTTGCCTTAATCGGCCCGATCTTTATCTTTTTACGCCAGAAAAAGGTGGCGGGTGTGTTGGCGATCTTTGGTTTTTACGGTGCTGCAATAACCTTATTTGGCGAGTTAATTTTTACTCCATTAAAACAAGAGGAGATTGTCAAATTTCTCTTTGTTGGTTTGGAAAACAACCAGGTTTACTTCATGATGCACTTCTTGAGTTTCTTGTTGAGTCTGGCTGTCTTTTTATGA", "aa": "MQSLNYLVVILTVAGVLVILGFTPLIRKLKIQFYCLQVFAAILFLYVFFGRQIIYIFPDIYGTAAKAKNAVANVPLDSLRLSRIFLLDLCPFFALIGPIFIFLRQKKVAGVLAIFGFYGAAITLFGELIFTPLKQEEIVKFLFVGLENNQVYFMMHFLSFLLSLAVFL", "aa_hexdigest": "1c2b5ac40e079a69c01ed1fba2dcbafb", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 325975, "stop": 326784, "observations": { "mutation": [ 326142, 326280 ], "stop": [ 326142, 326280 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75501", "query_cov": 1.0, "subject_cov": 0.5894736842105263, "identity": 1.0, "score": 313.0, "evalue": 3.54e-106, "gene_start": 1, "gene_end": 168, "reference_start": 1, "reference_end": 168, "reference_sequence": "MQSLNYLVVILTVAGVLVILGFTPLIRKLKIQFYCLQVFAAILFLYVFFGRQIIYIFPDIYGTAAKAKNAVANVPLDSLRLSRIFLLDLCPFFALIGPIFIFLRQKKVAGVLAIFGFYGAAITLFGELIFTPLKQEEIVKFLFVGLENNQVYFMMHFLSFLLSLAVFLWDDGFSLISFFYIHVFALAYLSYVALMVNIFKGQITGNTTGILAEDWLSGEYKNVAVFLKLDPKNADLIFGVSFGLSYFAIVLLTVLVNIPTFIQLTKDKQMVKLALQLKKAQASVA" }, "paralog": false, "identity": 0.9925650557620818, "score": 514.0, "evalue": 1.23e-187, "description": "Internal stop codon at 326142, 326280. Nonsense mutation around 326142, 326280.", "psc": { "uniref90_id": "UniRef90_P75501", "product": "Uncharacterized protein MG135 homolog", "uniref50_id": "UniRef50_P47381", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75501", "UniRef:UniRef50_P47381", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47381", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47381" ], "product": "Uncharacterized protein MG135" } }, "id": "APKECPOBEN_617", "locus": "GCA002090215_00581" }, { "type": "cds", "sequence": "contig_1", "start": 326829, "stop": 327254, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01336.31" ], "nt": "ATGAGCGATCGTTTAAATGATCAAGCACAAAATCGCTTGCAAAAACTCCTAAACCTCAAACAAACTGGCAACGATCCCTACCTCATTACTAAGATAGAAAACACCCACTCAGCGGCTAGTTTTCAAAAAGCATTTGCCAACCAATCTGACGCTGAGTTAAAGCAGTACCAAGTGATCTTAACCGGTCGGATTATTGCGCTCCGGCAGACCTTTATTATTATCCAAGACTTTAGTGGGAAAATGCAGTTGTACATTAATAAAAAAACTGCCCCCGAACTGTTTGAATATTTCAATAATTACATTGATTTGGGTGATCAAATAGTAGCCACTGGCCATCCAATGATGACCAAAACAGGTGTTTTAACGCTGAACGTAGAGCGATTACAAATTGTCGCTAAATGCTTACAAACCCCACCTGAAAAATGA", "aa": "MSDRLNDQAQNRLQKLLNLKQTGNDPYLITKIENTHSAASFQKAFANQSDAELKQYQVILTGRIIALRQTFIIIQDFSGKMQLYINKKTAPELFEYFNNYIDLGDQIVATGHPMMTKTGVLTLNVERLQIVAKCLQTPPEK", "aa_hexdigest": "313ab771441699e812771a1b274ce2a8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01336.31", "name": "tRNA_anti-codon", "length": 79, "aa_cov": 0.524822695035461, "hmm_cov": 0.9493670886075949, "evalue": 1.5082798931533074e-12, "score": 42.78839874267578, "start": 58, "stop": 131 } ], "seq_stats": { "molecular_weight": 16075.34399999999, "isoelectric_point": 9.014179039001466 }, "id": "APKECPOBEN_618", "locus": "GCA002090215_00582" }, { "type": "cds", "sequence": "contig_1", "start": 327261, "stop": 327707, "strand": "+", "frame": 3, "gene": null, "product": "amino acid--tRNA ligase-related protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000AFE6F12" ], "nt": "TTGACCGATCCAGAAGCGCGAGCACGAAAGCGTTACTTGGATTTGACCTACAACCGTGCGCAGGTCGAAGTCTTTTTGAAACGCACCCAGATTATTACAGCTATCCGTACCTTTTTAAATGAAGCGGGCTTTCTAGAAGTGGAAACACCCATTTTACAAGCCGTTTTAGGGGGTGCTAACGCCAAGCCCTTTAAAACACACTATAACGCCTTAAAGGGTGATTTTTACTTGCGGATTGCAAATGAAATAGCACTCAAAAAGCTCATTGTGGGCGGTTTGCCGAAAGTGTATGAAATGGGGCGGATGTTTCGTAACGAAGGGGTGGATACTACCCATAATCCGGAGTTTACGAGCATTGAAATGTATCAGGCTAACGCTGACTATGCGGTCATGATGGATTTAACCGAAAACCTCATTAAGTTTGTTTGCAAAACACTCAATCAGTGA", "aa": "MTDPEARARKRYLDLTYNRAQVEVFLKRTQIITAIRTFLNEAGFLEVETPILQAVLGGANAKPFKTHYNALKGDFYLRIANEIALKKLIVGGLPKVYEMGRMFRNEGVDTTHNPEFTSIEMYQANADYAVMMDLTENLIKFVCKTLNQ", "aa_hexdigest": "278eab2d7f0f5af08b4da585945887c5", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI000AFE6F12", "query_cov": 0.8918918918918919, "subject_cov": 0.8048780487804879, "identity": 0.598, "score": 160.0, "evalue": 9.83e-48, "valid": false, "product": "amino acid--tRNA ligase-related protein", "uniref50_id": "UniRef50_UPI000AFE6F12" }, "pscc": { "uniref50_id": "UniRef50_UPI000AFE6F12", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000AFE6F12" ], "product": "amino acid--tRNA ligase-related protein" }, "genes": [], "id": "APKECPOBEN_619", "locus": "GCA002090215_00583" }, { "type": "cds", "sequence": "contig_1", "start": 327765, "stop": 328298, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00152.25" ], "nt": "ATGGTAGATTTAATCTGCCAAGTTACTGGGGTTAATTTTGATGATGTTAAGGATGATCAAACAGCCATTGCGCTTGCAAAGCAGCACAAAGTAGAGTTAAAAAAGCACGAGCAGAACAAACAACACATTATTAACCGTTTCTTTGAACAGTTCTGTGAGCATACCTTAATACAACCCACCTTTGTCACACACTATCCCAAGGCCGTTTCCCCGCTAGCTAAACAAGATCCCCACAACCCCGAGTTTACTGAGCGCTTTGAACTTTTTATTAATACAAAAGAGTTAGCGAACGCTTACAGTGAGTTGAATGATCCCCTAGAACAACGTGCGCGCTTTGAACAACAGTTGCAGGAAAAACGCATGGGTAATGATGAAGCAAGTGAATTAGATGAATCCTTTTTGGATGCTTTGAGTTTTGGTCTCCCGCCAACAGGCGGCCTTGGGATTGGGGTTGATCGGTTAGTGATGTTATTGTGCGAGTGCAGTTCCATCCGTGACGTTGTTTTCTTTCCCCAACTACGTGAATTGAAATAA", "aa": "MVDLICQVTGVNFDDVKDDQTAIALAKQHKVELKKHEQNKQHIINRFFEQFCEHTLIQPTFVTHYPKAVSPLAKQDPHNPEFTERFELFINTKELANAYSELNDPLEQRARFEQQLQEKRMGNDEASELDESFLDALSFGLPPTGGLGIGVDRLVMLLCECSSIRDVVFFPQLRELK", "aa_hexdigest": "478c23a9d37a17f0a5bc9f0b749d0bb3", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0031D0E1C7", "query_cov": 0.9830508474576272, "subject_cov": 0.8093023255813954, "identity": 0.563, "score": 191.0, "evalue": 1.05e-58, "valid": false, "product": "amino acid--tRNA ligase-related protein", "ec_ids": [ "6.1.1.6" ], "uniref50_id": "UniRef50_A0A349PV97", "kegg_orthology_id": "K04567" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00152.25", "name": "tRNA-synt_2", "length": 127, "aa_cov": 0.6892655367231638, "hmm_cov": 0.968503937007874, "evalue": 1.2381542865080074e-37, "score": 125.01724243164062, "start": 52, "stop": 173 } ], "seq_stats": { "molecular_weight": 20361.885599999987, "isoelectric_point": 5.039197349548339 }, "id": "APKECPOBEN_620", "locus": "GCA002090215_00584" }, { "type": "cds", "sequence": "contig_1", "start": 328426, "stop": 329103, "strand": "+", "frame": 1, "gene": "glf", "product": "UDP-galactopyranose mutase", "db_xrefs": [ "COG:COG0562", "COG:M", "EC:5.4.99.9", "GO:0005829", "GO:0008767", "GO:0009273", "GO:0050660", "KEGG:K01854", "SO:0001217", "UniRef:UniRef50_P75499", "UniRef:UniRef90_P75499" ], "nt": "TTGGATGAAACCACCGGGTTACTGTTCCACCAGTACGGTCCTCATATCTTTCATACTGATAATCAGAAAGTAATGGATTTTATCCAACCCTTTTTTGAACTAAACAATTACCAGCACCGTGTTGGCTTACAACTGGACAACAATTTGGATTTAACGCTTCCATTTGACTTTAGCCAAATGCGCAAGTTGTTGGATACAAAAACAGCTAGCAGTTTAATTAACTTCTTTCAACAGCACTTTCCAGCTGAAAAGCACTTAACACTGATGCAGTTACAAACAATTAACTTTGCTCCAGTCCAACAGCTGTACCAGTTTTTAAAAATAAAGGTGTACGGTCCTTACAGTGTGAAAATGTGGGGCATGCCATTAGAACAAATTGATCCTAGTGTATTGGGTCGAGTCAAAATTAGTTTAAGTGAAAACAGTAGCTATTTCCCTACAGCCACTATTCAAGGTCTACCTAAGGGTGGTTATACAAAGGCCTTTACAAAGATGGTGGATCATCCTCTGATTGATTTAAGGTTAAACTGTCCAGCTAACTTAATTAGCGTAAACAATAATCAACTCTTGTTTGCAAATCAACCAATTACCAAACCAGTGGTTTATTGTGGTTTAATTGATCAGCTGTTTGGCTATTGTTTTGGGCGATTGCAGTACCGCTCGCTCCACTTTGAATGA", "aa": "MDETTGLLFHQYGPHIFHTDNQKVMDFIQPFFELNNYQHRVGLQLDNNLDLTLPFDFSQMRKLLDTKTASSLINFFQQHFPAEKHLTLMQLQTINFAPVQQLYQFLKIKVYGPYSVKMWGMPLEQIDPSVLGRVKISLSENSSYFPTATIQGLPKGGYTKAFTKMVDHPLIDLRLNCPANLISVNNNQLLFANQPITKPVVYCGLIDQLFGYCFGRLQYRSLHFE", "aa_hexdigest": "df9f935bab6af34d2ed203a060d59a84", "start_type": "TTG", "rbs_motif": null, "genes": [ "glf" ], "truncated": "both", "pseudogene": { "start": 328258, "stop": 329406, "observations": { "mutation": [ 328291, 329101 ], "stop": [ 328291, 329101 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75499", "query_cov": 1.0, "subject_cov": 0.5639097744360902, "identity": 0.996, "score": 465.0, "evalue": 2.09e-163, "gene_start": 1, "gene_end": 225, "reference_start": 57, "reference_end": 281, "reference_sequence": "MTLFSFPNYVNWNKFDFIVLGAGISGIVLSHVLAQHGKSVLLLEKRNQLGGNCYDKLDETTGLLFHQYGPHIFHTDNQKVMDFIQPFFELNNYQHRVGLQLDNNLDLTLPFDFSQMRKLLDTKTASSLINFFQQHFPAEKHLTLMQLQTINFAPVQQLYQFLKIKVYGPYSVKMWGMPLEQIDPSVLGRVKISLSENSSYFPTATIQGLPKGGYTKAFTKMVDHPLIDLRLNCPANLISVNNNQLLFANQPITKPVVYCGLIDQLFGYCFGRLQYRSLHFEWKRYAVKQHQAYPVMNWPLHPTITRQVEYKQLTQEGLESNQTIVSCETPGAFREGDPRFMEPYYPLNDVSNNALFARYLKLANAIPNIHLLGRLALYQYIDMDRAIAQSLAKAEQLLQ" }, "paralog": false, "identity": 0.9921465968586387, "score": 778.0, "evalue": 5.2e-289, "description": "Internal stop codon at 328291, 329101. Nonsense mutation around 328291, 329101.", "psc": { "uniref90_id": "UniRef90_P75499", "gene": "glf", "product": "UDP-galactopyranose mutase", "ec_ids": [ "5.4.99.9" ], "uniref50_id": "UniRef50_P75499", "cog_id": "COG0562", "cog_category": "M", "kegg_orthology_id": "K01854", "go_ids": [ "GO:0005829", "GO:0008767", "GO:0009273", "GO:0050660" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75499", "EC:5.4.99.9", "UniRef:UniRef50_P75499", "COG:COG0562", "COG:M", "KEGG:K01854", "GO:0005829", "GO:0008767", "GO:0009273", "GO:0050660" ] }, "pscc": { "uniref50_id": "UniRef50_P75499", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75499" ], "product": "UDP-galactopyranose mutase" } }, "id": "APKECPOBEN_621", "locus": "GCA002090215_00585" }, { "type": "cds", "sequence": "contig_1", "start": 329143, "stop": 329457, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACTGGCCACTCCACCCAACAATAACGCGCCAAGTGGAATACAAACAGTTAACGCAAGAGGGTTTGGAGTCTAACCAAACGATTGTTTCGTGTGAAACTCCTGGTGCTTTTAGGGAAGGCGATCCCCGCTTTATGGAGCCGTACTACCCGCTAAATGATGTAAGTAATAATGCCTTGTTTGCGCGTTATTTAAAGTTAGCTAACGCTATTCCTAACATACATTTGTTAGGTCGGTTGGCACTGTACCAGTACATTGATATGGATAGAGCAATTGCTCAAAGCTTAGCTAAAGCCGAACAACTATTACAGTAA", "aa": "MNWPLHPTITRQVEYKQLTQEGLESNQTIVSCETPGAFREGDPRFMEPYYPLNDVSNNALFARYLKLANAIPNIHLLGRLALYQYIDMDRAIAQSLAKAEQLLQ", "aa_hexdigest": "85fcaea78d001ecb592da9f15d933603", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11938.490099999986, "isoelectric_point": 5.580077171325683 }, "id": "APKECPOBEN_622", "locus": "GCA002090215_00586" }, { "type": "cds", "sequence": "contig_1", "start": 329457, "stop": 331253, "strand": "+", "frame": 3, "gene": "lepA", "product": "translation elongation factor 4", "db_xrefs": [ "EC:3.6.5.n1", "GO:0003746", "GO:0003924", "GO:0005525", "GO:0005886", "GO:0043022", "GO:0045727", "GO:1990606", "RefSeq:WP_015344898.1", "SO:0001217", "UniParc:UPI000012E47D", "UniRef:UniRef100_P75498", "UniRef:UniRef50_B1X3K4", "UniRef:UniRef90_P75498" ], "nt": "ATGGAACAACAAAAAATTAGGAACTTTTCAATTATTGCCCACATTGACCATGGTAAGTCAACGCTGTCAGATCGCTTAATTGAACGCAGCATTGGCTTTGAAAAACGCTTACTGCAAGCGCAAATGTTGGACACCATGGCGATTGAGCGTGAACGGGGTATTACTATTAAACTCAACGCCGTGCAGCTCAAAATGGCCCAAGGCAACCAGCAGTACTTGTTTCACCTAGTTGATACTCCGGGACACGTTGACTTTACTTACGAGGTATCACGCTCTTTAGCAGCTTGTGAGGGTGTTTTACTGTTAGTTGATGCTACCCAAGGGATTCAAGCACAAACGATTTCCAACACTTACTTAGCATTGGAAAACAACCTCGAAATTATTCCGGTAATTAACAAGGTGGACATGGAGAGTGCTGATGTCGAGAAAACCAAACAAGCCTTTCACCAACTGCTAGGGGTGGATCCCAACACCATCCCCTTAGTGTCCGCTAAAACCGGGCTTGGTATTGACCAGTTAATCACTACCATTATTGAAAAAGTACCCCCTCCAAAGGGCGATGAAAGTAAACCACTCAAAGCCTTACTGTTTGACAGCTATTACGATCCTTATAAGGGGGTAGTGTGCTTTATTCGAATCTTTGAGGGGAGTTTAAAACTCAATGATAAGATCCGCTTTGCTAGAAGTAACTCGGTTTACCAAATTGTGGAATTGGGAATTAAAAACCCCTTTTTTGAAAAACAAGACGTGTTAAAAGCCGGGGAGATTGGTTGGTTTTCCGCTGGGATTAAAAAACTAAGGGATGTAACGGTTGGTGACACGATTGTTCACGCTGAGGACACGACCACTCCACCCTTACCGGGTTACAAAAAGGTGTTACCGATGATTTACTGTGGTCTGTACCCGATTGACAACAATGATTACCAAAACCTCAAAATGGCCATGGAAAAGATTATTTTGAGCGATGTGGCCTTGGAGTATGAATATGAAACATCACAAGCATTGGGCTTTGGGGTGCGTTGTGGTTTTTTGGGGTTGTTGCACATGGATGTAATTAAGGAGCGGTTGGAGCGCGAGTACAACCTTAAGTTAATTTCCGCTCCTCCTTCAGTGCGGTATAAGGTGTTGTTAACCAACGGGGAGGAATTGGAACTCGATAACCCTTCACTCTTACCAGAGCGCTCGCGAATTAAATCAATTAGCGAACCGTTTGTACGCGTTTACATTGATCTACCCGATCATTATTTAGGTACAGTAATTGATCTGTGTCAAAACTTCCGTGGGCAATACGAAAAGTTAGAGGAAATTGACATTGACCGCAAACGCTTGGTCTACTTAATGCCCTTGGGTGAAATTATCTACAGTTTCTTTGATAAGTTGAAATCAATCACTAAGGGTTATGCTTCGCTCAATTACGAGTTTGACCAGTACCAGGTGTCCCAATTGGCTAAGGTGGAAATTATGTTAAACAAACAGAAGGTGGACGCTTTATCTTTTATTGCCCACCACGACTTTGCCTTTCAACGGGCCAAGAAGTTCTGTGTCAAATTAAAGGAACTAATTCCCAAGCACCTGTTTGAAATTCCAATTCAAGCCACAATTGGAAGTAAGGTAATTGCTAGAGAAACGATTAAAGCAGTGCGTAAAGATGTAACAGCAAAGCTGTATGGCGGGGATGTCACGCGGAAAAAGAAGCTGTTGGAAAAACAAAAGGAAGGGAAGAAAAGACTGAAAGCAATTGGTAGTGTGGAGCTCCCCCAAGAGCTGTTTAGTCATTTGCTCAAAGATGAAGATTAA", "aa": "MEQQKIRNFSIIAHIDHGKSTLSDRLIERSIGFEKRLLQAQMLDTMAIERERGITIKLNAVQLKMAQGNQQYLFHLVDTPGHVDFTYEVSRSLAACEGVLLLVDATQGIQAQTISNTYLALENNLEIIPVINKVDMESADVEKTKQAFHQLLGVDPNTIPLVSAKTGLGIDQLITTIIEKVPPPKGDESKPLKALLFDSYYDPYKGVVCFIRIFEGSLKLNDKIRFARSNSVYQIVELGIKNPFFEKQDVLKAGEIGWFSAGIKKLRDVTVGDTIVHAEDTTTPPLPGYKKVLPMIYCGLYPIDNNDYQNLKMAMEKIILSDVALEYEYETSQALGFGVRCGFLGLLHMDVIKERLEREYNLKLISAPPSVRYKVLLTNGEELELDNPSLLPERSRIKSISEPFVRVYIDLPDHYLGTVIDLCQNFRGQYEKLEEIDIDRKRLVYLMPLGEIIYSFFDKLKSITKGYASLNYEFDQYQVSQLAKVEIMLNKQKVDALSFIAHHDFAFQRAKKFCVKLKELIPKHLFEIPIQATIGSKVIARETIKAVRKDVTAKLYGGDVTRKKKLLEKQKEGKKRLKAIGSVELPQELFSHLLKDED", "aa_hexdigest": "21c09304fb422e3d6a0d4bf37aa136f2", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000012E47D", "ncbi_nrp_id": "WP_015344898.1", "uniref100_id": "UniRef100_P75498", "db_xrefs": [ "SO:0001217", "UniParc:UPI000012E47D", "RefSeq:WP_015344898.1", "UniRef:UniRef100_P75498" ] }, "ips": { "uniref100_id": "UniRef100_P75498", "uniref90_id": "UniRef90_P75498" }, "psc": { "uniref90_id": "UniRef90_P75498", "gene": "lepA", "product": "translation elongation factor 4", "ec_ids": [ "3.6.5.n1" ], "uniref50_id": "UniRef50_B1X3K4", "go_ids": [ "GO:0003746", "GO:0003924", "GO:0005525", "GO:0005886", "GO:0043022", "GO:0045727", "GO:1990606" ] }, "genes": [ "lepA" ], "id": "APKECPOBEN_623", "locus": "GCA002090215_00587" }, { "type": "cds", "sequence": "contig_1", "start": 331332, "stop": 333035, "strand": "+", "frame": 3, "gene": "rnj", "product": "Ribonuclease J", "db_xrefs": [ "COG:COG0595", "COG:J", "EC:3.1.-.-", "GO:0003723", "GO:0004521", "GO:0004534", "GO:0005737", "GO:0006364", "GO:0008270", "SO:0001217", "UniRef:UniRef50_P75497", "UniRef:UniRef90_P75497" ], "nt": "ATGATTAAGGATTTTGATCCTAGTGAGTTTGTCGGTAAAACTCCTACCAAGATTTTTGCCTTTGGTGGTATCCAAGAAGTTGGAAAAAACATGTATGGAATCGAGTACGATGATGAGATTATCATCATCGACTGCGGTATTAAGTTTGCTAGTGATGATCTACTAGGGATTGATGGTATTATCCCGAGCTTCGAATACCTCATTGAAAACCAAGCTAAGGTTAAAGCACTGTTTATCACCCACGGCCATGAAGACCACATTGGTGGGGTGCCTTACTTGTTAAAACAAGTCGATGTACCGGTGATTTACGCACCCCGGATTGCCGCTTCTTTGATCTTAAAGAAGGTCAACGAACACAAGGATGCTAAGCTCAATAAAGTGGTCGTATATGATGACTTTAGCAACTTTGAAACCAAGCACTTCAAGATTGACTTTTACCGCGTCAACCACTCGATTCCTGACGCCTTTGGTGTCTGTGTTCAAACTCCCAACGGTAACATTGTTGAGAGTGGTGACTTTCGCTTTGACTTTGCCGCTGGCGGGGAAATGCTCGATGTGCATAAGGTCGTGAAGATTGCCGAGCGCAATGTCCATGTCTTTATGTGTGAGACTACTAACGCCGAAATCCCGGGGTTCTCCCAGAGCGAAAAGTTAATTTACCGCAACATCAACAAGATTATTAAAGAAGCACGTGGTCGGGTCATTCTCACTACCTTTGCTTCCAACATTACCCGGATTAACGAAATTATTGAGATTGCTGTTAACAATAAACGGAAGGTCTGCTTGTTGGGTAAGTCGATGGATGTGAACGTCAACATCTCGCGTAAGATTGGCTTAATGGACATTGACAGCAATGACATTGTGGAAGTCCGTGACATTAAGAATTACCCCGACCGCAGTATCTTAATCTTGTGTACGGGTTCCCAAGGTGAAGACTCAGCAGCCTTAAACACAATGGCACGGGGTAAACATAACTGGGTTAGCTTAAAGTCCACTGACACCATTATTATGTCCTCCAACCCGATTCCGGGGAACTATGCAGCGGTGGAAAATCTCTTGAATGAGCTTTCGAAGTACGGGGTGACCATCTTTGAAAACTCACCGAACATGAAGCTACACGCCTCTGGTCACGCTACCCAGCAAGAGTTGCAGTTAATGTTAAACCTCGTGTTTCCAAGGTACTTAATTCCGATTCACGGGGAGTACAAGATGATGCGCACCATCAAAAACATAGCGCAAGAATGTGGCATTAACGGTGATGATGTTGGTTTGCTAGCTAACGGTCAGGTGATGTACCTAATTGATGGCAAGCTGTATTACTCAGGGGAAGTGATTAATGCTGACCCGATTTACATTGAAAGCCGCAATTCTTCTCCAGACTTAGCGCGGGTAATTAAACAACGCCAAATCTTGAGTCGGGAGGGAATGTTTGCTGTGATTGTAGTCTTTGACAAAAACAATAACATTTTAGGGATGCCTACTCTGATTACTCGGGGTTGTTTCTTTGCTCTAGATTCCAGTCCGTTAATGACTAAGATTACCCACTCAATTAAACGCGGTTTGGAAAACGTTATTCAAAACAAGCGTTTTAATACCCGTGAACAGATGATCAAGGAATTGAAACGAGTATGTAAGGAAACGGTGTCTTACTTCATTTGGAAGAACAAGAGTCGTAACCCGTTGATTTCAACCGTATTGTCTTGA", "aa": "MIKDFDPSEFVGKTPTKIFAFGGIQEVGKNMYGIEYDDEIIIIDCGIKFASDDLLGIDGIIPSFEYLIENQAKVKALFITHGHEDHIGGVPYLLKQVDVPVIYAPRIAASLILKKVNEHKDAKLNKVVVYDDFSNFETKHFKIDFYRVNHSIPDAFGVCVQTPNGNIVESGDFRFDFAAGGEMLDVHKVVKIAERNVHVFMCETTNAEIPGFSQSEKLIYRNINKIIKEARGRVILTTFASNITRINEIIEIAVNNKRKVCLLGKSMDVNVNISRKIGLMDIDSNDIVEVRDIKNYPDRSILILCTGSQGEDSAALNTMARGKHNWVSLKSTDTIIMSSNPIPGNYAAVENLLNELSKYGVTIFENSPNMKLHASGHATQQELQLMLNLVFPRYLIPIHGEYKMMRTIKNIAQECGINGDDVGLLANGQVMYLIDGKLYYSGEVINADPIYIESRNSSPDLARVIKQRQILSREGMFAVIVVFDKNNNILGMPTLITRGCFFALDSSPLMTKITHSIKRGLENVIQNKRFNTREQMIKELKRVCKETVSYFIWKNKSRNPLISTVLS", "aa_hexdigest": "d21bd145e41819c6527a6f3dc701c2a8", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75497", "query_cov": 1.0, "subject_cov": 0.9964850615114236, "identity": 1.0, "score": 1127.0, "evalue": 0.0, "valid": true, "gene": "rnj", "product": "Ribonuclease J", "ec_ids": [ "3.1.-.-" ], "uniref50_id": "UniRef50_P75497", "cog_id": "COG0595", "cog_category": "J", "go_ids": [ "GO:0005737", "GO:0004534", "GO:0003723", "GO:0004521", "GO:0008270", "GO:0006364" ] }, "pscc": { "uniref50_id": "UniRef50_P75497", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75497" ], "product": "Ribonuclease J" }, "genes": [ "rnj" ], "id": "APKECPOBEN_624", "locus": "GCA002090215_00588" }, { "type": "cds", "sequence": "contig_1", "start": 333252, "stop": 333881, "strand": "+", "frame": 3, "gene": null, "product": "P68 family surface lipoprotein", "db_xrefs": [ "GO:0005886", "GO:0009897", "SO:0001217", "UniRef:UniRef50_P75496", "UniRef:UniRef90_P75496" ], "nt": "ATGAAATTTAAGTATGGAACGGTTGTTTTGGGCAGTTTTTTAGGGCTTTCTGTGGTGTTAGCTGCCTGTGGTGCTCGTGGTAAATTTGACCAATTTGATGATGGCAAAATTAAGCTAGCATCATCCTTAACTTCCAAAGCAGCTGCCAATGCTTTACAAACAATAATAGAGAAGTACAACATTGTCAAAAGCGGCAAGGATTACCCCATTGAAATTACCCAAATTGCTGGTGGTTATGACGGCGGGCGGACTGATCTCCAAACCCGAGTTAGTGTTAAGGATAAAACGAACTTTTATAACCTTATTTTGAACTATCCAGATTTAGTTTCTGTGTTAGCGCGCAGTGGAATGGAACTGCTTTTTGACAAAGTTAATGTTGATAAATTAGAGCCGAACTTCTTAAAGTTTAATGAACAGATTAGTGGAGTCGCTAAAAGTGGTAACTATGGTATTCCCGTGTCCCTTTCAACTGACATTTTGGTCTTAAATGGTCCGGTGTTGCACTATATTTTGAACAGTGCGAAGAAAGAAGAGGCTAATTCTCAAGTTAAAAGTCAAATAAAAACATCACAAATTCAAGCTAAAGGTTCATTAACAATTGATACAGATGAAAATACCAAATCACTTTGA", "aa": "MKFKYGTVVLGSFLGLSVVLAACGARGKFDQFDDGKIKLASSLTSKAAANALQTIIEKYNIVKSGKDYPIEITQIAGGYDGGRTDLQTRVSVKDKTNFYNLILNYPDLVSVLARSGMELLFDKVNVDKLEPNFLKFNEQISGVAKSGNYGIPVSLSTDILVLNGPVLHYILNSAKKEEANSQVKSQIKTSQIQAKGSLTIDTDENTKSL", "aa_hexdigest": "c1111ab55381545f965bdb3047286aef", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 333252, "stop": 334184, "observations": { "mutation": [ 333879, 334050 ], "stop": [ 333879, 334050 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75496", "query_cov": 1.0, "subject_cov": 0.5543766578249337, "identity": 1.0, "score": 401.0, "evalue": 9.03e-139, "gene_start": 1, "gene_end": 209, "reference_start": 1, "reference_end": 209, "reference_sequence": "MKFKYGTVVLGSFLGLSVVLAACGARGKFDQFDDGKIKLASSLTSKAAANALQTIIEKYNIVKSGKDYPIEITQIAGGYDGGRTDLQTRVSVKDKTNFYNLILNYPDLVSVLARSGMELLFDKVNVDKLEPNFLKFNEQISGVAKSGNYGIPVSLSTDILVLNGPVLHYILNSAKKEEANSQVKSQIKTSQIQAKGSLTIDTDENTKSLWEKIQNSAKANGETNQKGRKAAKSNKTALVQLKNGADTTTNEENKDTKTSDDKVKQTWGDYVEKDDGLKNYTFRASVFENWHDFLDFSTRVAKSFTEKVSNITNKKGTDIQGVLGVDSSPNVLFASVFAAGGGNYENFFYKLKDGRADFSNFKNKGSSYQNLQSVFKD" }, "paralog": false, "identity": 0.9935483870967742, "score": 599.0, "evalue": 4.03e-219, "description": "Internal stop codon at 333879, 334050. Nonsense mutation around 333879, 334050.", "psc": { "uniref90_id": "UniRef90_P75496", "product": "P68 family surface lipoprotein", "uniref50_id": "UniRef50_P75496", "go_ids": [ "GO:0005886", "GO:0009897" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75496", "UniRef:UniRef50_P75496", "GO:0005886", "GO:0009897" ] }, "pscc": { "uniref50_id": "UniRef50_P75496", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75496" ], "product": "Uncharacterized lipoprotein MPN_281" } }, "id": "APKECPOBEN_625", "locus": "GCA002090215_00589" }, { "type": "cds", "sequence": "contig_1", "start": 334107, "stop": 334385, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTTGAAAACTGGCATGATTTCCTCGATTTTTCGACAAGGGTAGCTAAATCTTTTACTGAAAAGGTAAGCAACATTACCAATAAAAAGGGCACTGATATTCAGGGAGTGCTTGGTGTCGACAGTTCTCCTAATGTTTTATTTGCCTCTGTATTTGCTGCAGGTGGTGGTAATTATGAAAACTTCTTTTATAAGTTGAAAGATGGTCGGGCTGATTTTAGTAACTTTAAGAACAAGGGTAGTTCTTACCAAAACCTCCAAAGCGTTTTTAAAGACTAA", "aa": "MFENWHDFLDFSTRVAKSFTEKVSNITNKKGTDIQGVLGVDSSPNVLFASVFAAGGGNYENFFYKLKDGRADFSNFKNKGSSYQNLQSVFKD", "aa_hexdigest": "3df4d15a7e8287575d8fb3b577c324b5", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10321.302699999993, "isoelectric_point": 7.965665245056153 }, "id": "APKECPOBEN_626", "locus": "GCA002090215_00590" }, { "type": "cds", "sequence": "contig_1", "start": 334530, "stop": 334808, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAGTTTAGTGACGACAACACCTTCGTTGAATATCCTGATTTTACTCAGGAAATTAAGTCGCCAGACCAACAAAATGGTCAAAATACTGATGACAATAACAGTAAAAAAGAAGAAAACCTTTTAGGTACCTTTGAAGTTAAAGATTACTCGAAATCTAATAAAGGCGGGAAAGGAAAAAGTAAGGGTAAAGGCGGAAGCAGCAGTAATATGAAGACTATTTACATTTACAAAGGCTCTATTCCTGAAGATAAAAAGAAAGGTGATAATGCCATTTGA", "aa": "MKFSDDNTFVEYPDFTQEIKSPDQQNGQNTDDNNSKKEENLLGTFEVKDYSKSNKGGKGKSKGKGGSSSNMKTIYIYKGSIPEDKKKGDNAI", "aa_hexdigest": "d02542953b80cf220f31df956437a409", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10222.057099999995, "isoelectric_point": 7.827573966979982 }, "id": "APKECPOBEN_627", "locus": "GCA002090215_00591" }, { "type": "cds", "sequence": "contig_1", "start": 335336, "stop": 335611, "strand": "+", "frame": 2, "gene": null, "product": "Lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75594", "UniRef:UniRef90_A0AAN4XG79" ], "nt": "TTGCCCTTAAAAGAAAACTTTGAAAAGGTTCAAATTAACGATAAAGGTGAGAAAAATGAAAATGACACAAAGGGCAATACTTATACCGCTAAAGCTTTAGAACTCTTTAAAAAAGTTTCTCAAAATGAAATCGTGAGTTACAGTGACCCAAGTGACTTTAGAAGTGGTAAGTTTCGTGATGCGATTGGCTCTAACTTTAACGCCATTATTAATTCCAAAGGAGACTTCAATAAATTTATTAGTAGTTTTACCGCAAGTTTAGGCTCAGACTTTTAG", "aa": "MPLKENFEKVQINDKGEKNENDTKGNTYTAKALELFKKVSQNEIVSYSDPSDFRSGKFRDAIGSNFNAIINSKGDFNKFISSFTASLGSDF", "aa_hexdigest": "f0d0eb731e90b937640d853f7361250a", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 335231, "stop": 335611, "observations": { "mutation": [ 335255 ], "stop": [ 335255 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAN4XG79", "query_cov": 1.0, "subject_cov": 0.7222222222222222, "identity": 0.9890000000000001, "score": 175.0, "evalue": 5.05e-55, "gene_start": 1, "gene_end": 91, "reference_start": 36, "reference_end": 126, "reference_sequence": "MVLNTPETWESKRNKKNSNEKQTAAQYFAQEASYILPLKENFEKVQINDKGEKNENDTKGNTYTAKALELFKKVSQNEIVSYSDPSDFRSGKFRDAIGSNFNAIINSKGDFNKFISSFTASLGSDF" }, "paralog": false, "identity": 0.9841269841269841, "score": 246.0, "evalue": 1.27e-85, "description": "Internal stop codon at 335255. Nonsense mutation around 335255.", "psc": { "uniref90_id": "UniRef90_A0AAN4XG79", "product": "Lipoprotein", "uniref50_id": "UniRef50_P75594", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAN4XG79", "UniRef:UniRef50_P75594" ] }, "pscc": { "uniref50_id": "UniRef50_P75594", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75594" ], "product": "Uncharacterized lipoprotein MPN_098" } }, "id": "APKECPOBEN_628", "locus": "GCA002090215_00592" }, { "type": "cds", "sequence": "contig_1", "start": 336136, "stop": 336453, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD6513", "UniRef:UniRef100_A0A0H3DL71", "UniRef:UniRef50_A0A0H3DL71", "UniRef:UniRef90_A0A0H3DL71" ], "nt": "TTGAAGTTATTTTGTCACATTTACTTTTTGGGCAATTTAGATCATGAATACCGAAAGAGTATAAGATTCATTAAGTTAACGAGGTATCACCTCTTTTTAAAACAAGCCCAATCGCAACTCGTTTCTTTGTTTAACCAAAACTTTTTGCAAGTTTTGTTGGCATTTCTAAATAAATCTTTCAATTTATTTAGTGATGACACAAAAATTAATATGTTGAACTTTTTAAATAAATCAACAATGGTATTGATCCCCGTTGGCAGCAGTGCTCCAAGTTCTAGTTCCTTTTTAAATCACCTCAAGAAAGAAGGTATTGCTTAA", "aa": "MKLFCHIYFLGNLDHEYRKSIRFIKLTRYHLFLKQAQSQLVSLFNQNFLQVLLAFLNKSFNLFSDDTKINMLNFLNKSTMVLIPVGSSAPSSSSFLNHLKKEGIA", "aa_hexdigest": "a72389eee8571bf51ad05d84a99f5ac5", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD6513", "uniref100_id": "UniRef100_A0A0H3DL71", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD6513", "UniRef:UniRef100_A0A0H3DL71" ] }, "ips": { "uniref100_id": "UniRef100_A0A0H3DL71", "uniref90_id": "UniRef90_A0A0H3DL71" }, "psc": { "uniref90_id": "UniRef90_A0A0H3DL71", "uniref50_id": "UniRef50_A0A0H3DL71" }, "pscc": { "uniref50_id": "UniRef50_A0A0H3DL71", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0H3DL71" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12175.143799999983, "isoelectric_point": 9.868578529357908 }, "id": "APKECPOBEN_629", "locus": "GCA002090215_00593" }, { "type": "cds", "sequence": "contig_1", "start": 336453, "stop": 336563, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGAGAAAATTTCTAAAAAGGAATACAATGCCTTAATCCGTAAAACTGGCGAAAAGCACTTTGACGGCGAAAAGGAAGAGTACGGTGATGGCACCGTTGGACTTTGA", "aa": "MKEKISKKEYNALIRKTGEKHFDGEKEEYGDGTVGL", "aa_hexdigest": "d16543a5863734ff111d693cff88e39d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4129.604900000002, "isoelectric_point": 6.5516260147094725 }, "id": "APKECPOBEN_630", "locus": "GCA002090215_00594" }, { "type": "cds", "sequence": "contig_1", "start": 336600, "stop": 336800, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01519.22" ], "nt": "GTGAAAGTTAAATACGTAACGCAAGAAGAGTTTGGCGAATTCAAAGATGCCACCAACCAGCGGCTCACAAAGATTGAGAACGCTTTGGTCGCTCAGGGCGAACAAATTAGAGCTCAGGGTGAGCAATTAAGTCAACTAATTAAAGTTGTGCTTCTTCAGGGCGAACAAATAAAATCTCAAGGTGAGCAAATAAAATCTTAG", "aa": "MKVKYVTQEEFGEFKDATNQRLTKIENALVAQGEQIRAQGEQLSQLIKVVLLQGEQIKSQGEQIKS", "aa_hexdigest": "2ed90d23528a2752f49bba0568ee47cc", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01519.22", "name": "DUF16", "length": 64, "aa_cov": 0.9696969696969697, "hmm_cov": 1.0, "evalue": 2.7241689427528485e-15, "score": 52.22478485107422, "start": 2, "stop": 65 } ], "seq_stats": { "molecular_weight": 7476.435699999999, "isoelectric_point": 6.217981147766112 }, "id": "APKECPOBEN_631", "locus": "GCA002090215_00595" }, { "type": "cds", "sequence": "contig_1", "start": 337238, "stop": 337486, "strand": "+", "frame": 2, "gene": null, "product": "Family K-like protein", "db_xrefs": [ "RefSeq:WP_015344899.1", "SO:0001217", "UniParc:UPI0002B8464C", "UniRef:UniRef100_A0AAP8JFL4", "UniRef:UniRef50_A0AAP8E4D4", "UniRef:UniRef90_A0AAP8JFL4" ], "nt": "ATGAAAAAAGTTAAAGTTCTAGAGAAGCCATCTGTTGAAAGAATGATAGAAGTTTTAAGCGACTCTGAAATATTGAAGTTTGATTTTTCTTGCTATTATTTACCTGATGGTACTAATGAGACGCTCATAAGAATCTTTTTTCAAGAAAAAGTTTCAAGTTCTGTAGTAACAAAAGATAAAAATTCTTTAAGACTTGATGAATACTTAAAAGACTTTATTAGCTTCTTAAAAACACAAAGAAAAAACTAA", "aa": "MKKVKVLEKPSVERMIEVLSDSEILKFDFSCYYLPDGTNETLIRIFFQEKVSSSVVTKDKNSLRLDEYLKDFISFLKTQRKN", "aa_hexdigest": "6dad6782f1b05f336bf0127f2ff65eb9", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0002B8464C", "ncbi_nrp_id": "WP_015344899.1", "uniref100_id": "UniRef100_A0AAP8JFL4", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002B8464C", "RefSeq:WP_015344899.1", "UniRef:UniRef100_A0AAP8JFL4" ] }, "ips": { "uniref100_id": "UniRef100_A0AAP8JFL4", "uniref90_id": "UniRef90_A0AAP8JFL4" }, "psc": { "uniref90_id": "UniRef90_A0AAP8JFL4", "product": "Family K-like protein", "uniref50_id": "UniRef50_A0AAP8E4D4" }, "pscc": { "uniref50_id": "UniRef50_A0AAP8E4D4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAP8E4D4" ], "product": "Family K-like protein" }, "genes": [], "id": "APKECPOBEN_632", "locus": "GCA002090215_00596" }, { "type": "cds", "sequence": "contig_1", "start": 337744, "stop": 338526, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATTGAAATATGGAACCATTATTTTCAGTGGTCTTTTGGGAGTTTCCGCCATTTTAGCTGCCTGTGGTGCACGCGGCAAATTTGACCAAGTTGATGATGGCAAAATTGTTTTAGCTTCTTCTCTTACATCTAAGGGTGCAGCTAATGCCTTGCAAACAATTGTTAAAAAGTACAACGAAGTTAAAAATATTGATGACTATCCCATTGAAATTATCCAAATTGCCGGTGGTTATGATGGCGGTCGTGGTAACTTACAAACAAAACTAAGTGTTAAAGACAAAAATAGTTTCTATAACTTAATTTTGAACTACCCAGACGTAGTTTCTGTTTTAGGTCGAGTAGGCATGGAATTGCCCTTCGATAAGGTTAGAACAGATAAATTATCACCCCGTTTTCTTGATTTCAACAAGCGAATTAGTGCCATATCTAAGCAAGGTATTTACGGCATTCCAGTCTCTTTGTCTACTGAAGTTTTAGTTTTAAATGGTCCGGTATTGCACTATATTTTGAGCAGTGCAAAGGGTAGTAGTGGCAAAACGCAAGTAAGTCAAACATCATCAGGATCAAATCAACAAAAAACTCTTCAAAAACCCTTAAAGATTGATACAAGCGATTCAAGCACTTCTTCTCTTTGGACTCAAATTGAGAACGCCGCTAAAAATAACGGTAAAAAAGCAAATAATAGCAAAAGCAATAGAAGATCCACAGATCAAAGTACACAAACACATAACGATCAAGGCGATGCTTCTGAAAGTGATAAAAAGATTAAAGAATCTTGA", "aa": "MKLKYGTIIFSGLLGVSAILAACGARGKFDQVDDGKIVLASSLTSKGAANALQTIVKKYNEVKNIDDYPIEIIQIAGGYDGGRGNLQTKLSVKDKNSFYNLILNYPDVVSVLGRVGMELPFDKVRTDKLSPRFLDFNKRISAISKQGIYGIPVSLSTEVLVLNGPVLHYILSSAKGSSGKTQVSQTSSGSNQQKTLQKPLKIDTSDSSTSSLWTQIENAAKNNGKKANNSKSNRRSTDQSTQTHNDQGDASESDKKIKES", "aa_hexdigest": "394fcb8589be62a45b3e0278e5e585ef", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 28066.369700000017, "isoelectric_point": 9.551780891418456 }, "id": "APKECPOBEN_633", "locus": "GCA002090215_00597" }, { "type": "cds", "sequence": "contig_1", "start": 338542, "stop": 339744, "strand": "+", "frame": 1, "gene": null, "product": "P80 family lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001330F80B", "UniRef:UniRef90_UPI001330F80B" ], "nt": "GTGGATGGTGGTTTAAAAGGCTTTACTTTTAAAGCTAGCATCTTTGATAATTGGCACGATTTACTCGATTTTTCTACTAGAGCGGCTAAATCTTTTAAAAAGATAAAAGATAACAATACTAAAAAAGGAACTGATATCCAAGGCATTCTTGGTGTAGATAGTTCAGCCAATTCCTTATTTACTTCTGTTTTTGCAGCTGGTAATGGTGACTATGACAACTTCTTTTACAAGGTTGCAAACGGTCGGGCTGACTTTAGTAACTTTAAGAATAGAGGTAGTTCCTTCCAAAACTTACAAAGTGTTTTTAATGATTACAAGGGTTTAATTGATCAAAACGGTCTCTTTGTTAATAAGGGCGGTTCTTACTCTTCTAACTTTCAAAAGTTTCACCAGTTAGCTTACAGTATCTCCTCTACCTCTGGTTTCTATTATTCATTTGCTGGTAATAGTGCTAAGCGCTTAAAGTTTGGTGATAATTCTTTCATTGAGTATCCACAATATACTGTTCCAATTAAAGCTCCTTCAAAGAATGGTGATGGAAATAGCACAAACAGCAATAGCGATCTTTTAGGCACTTTCACTTTAAGTAGTGTTAAAAAGTCAACAGATAAAAGTAAAAGTGATAGTCAACAAAATCAAGGCAAGAAAGTAGAAGGAACACCTAACCAGGGTAAAAAGGCAGAAGGTGCCCAGAACCAAGGCAAAAAAGAAAATAACAGTACAACTATTGAAATTTACAAGAACAAAATTCCTGATGGTAAAAATGCTGGGAAAGATGCCATCTTAATAAAAGATAACAATCTCATTAAACAGCTAGAAGATGCTGCTAAGAAAAACGGTGCAGAAAGTAATCAAAAACAAGGTGGAGAGAGTAATGTTCAGAAAGAACAAATTATTGGTTATACCACCACTGGTAACGTTCGTGAAGATGGTAATCACATTTTTAGAGTAGACAAAATTAATGACGAACAATACGATCGCAAAATTATTGTTGGCGTTACTGTGGAAACTTTAGAACAGTCTAGCACTTTGCAAAGCGAAGAAGCAATTGTTTTAGCAGCACCTGGTAAATATAAAAGCACTGATAAAAAGAAGGTAACAATTACCCAAGGTCCGAATATTATTGGTATCCATGCTAACGAAAAAGAAAATGCAGAAACCCAAAAGTTTGTTGACTGGTTTTTAAATACAGAAGTAGATTGA", "aa": "MDGGLKGFTFKASIFDNWHDLLDFSTRAAKSFKKIKDNNTKKGTDIQGILGVDSSANSLFTSVFAAGNGDYDNFFYKVANGRADFSNFKNRGSSFQNLQSVFNDYKGLIDQNGLFVNKGGSYSSNFQKFHQLAYSISSTSGFYYSFAGNSAKRLKFGDNSFIEYPQYTVPIKAPSKNGDGNSTNSNSDLLGTFTLSSVKKSTDKSKSDSQQNQGKKVEGTPNQGKKAEGAQNQGKKENNSTTIEIYKNKIPDGKNAGKDAILIKDNNLIKQLEDAAKKNGAESNQKQGGESNVQKEQIIGYTTTGNVREDGNHIFRVDKINDEQYDRKIIVGVTVETLEQSSTLQSEEAIVLAAPGKYKSTDKKKVTITQGPNIIGIHANEKENAETQKFVDWFLNTEVD", "aa_hexdigest": "a1daa974218e0a05c0a516e08a397047", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001330F80B", "query_cov": 1.0, "subject_cov": 0.8528784648187633, "identity": 0.993, "score": 730.0, "evalue": 5.58e-264, "valid": true, "product": "P80 family lipoprotein", "uniref50_id": "UniRef50_UPI001330F80B" }, "pscc": { "uniref50_id": "UniRef50_UPI001330F80B", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001330F80B" ], "product": "P80 family lipoprotein" }, "genes": [], "id": "APKECPOBEN_634", "locus": "GCA002090215_00598" }, { "type": "cds", "sequence": "contig_1", "start": 340218, "stop": 340397, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01420.25" ], "nt": "GTGGAGATTAAAACTTACAAAATCAAAGATATTTGCGATATTAAACGCGGTAGAGTTATCAGCAAATTGGACATTAAAAAAGATCCAGGAGTTTTTCCTGTTTATTCGGCAGCTACTAATAACGATGGGGAGTTTGGTCGAATAAATTCTTATGACTTTGATGGTGAATATGTTACTTGA", "aa": "MEIKTYKIKDICDIKRGRVISKLDIKKDPGVFPVYSAATNNDGEFGRINSYDFDGEYVT", "aa_hexdigest": "6973df725003fab248d0403e95c16b3e", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01420.25", "name": "Methylase_S", "length": 61, "aa_cov": 0.9661016949152542, "hmm_cov": 0.9836065573770492, "evalue": 3.745638772505241e-11, "score": 38.95970153808594, "start": 3, "stop": 59 } ], "seq_stats": { "molecular_weight": 6735.564300000002, "isoelectric_point": 6.130108070373535 }, "id": "APKECPOBEN_635", "locus": "GCA002090215_00599" }, { "type": "cds", "sequence": "contig_1", "start": 340378, "stop": 340473, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTGAATATGTTACTTGAACAGCTGACGGTTATGGAGGGGCTGTTTTTTATCGTAATGGTAAATTTAGCATCACAAATCTTTGTGGTCTTTTGA", "aa": "MVNMLLEQLTVMEGLFFIVMVNLASQIFVVF", "aa_hexdigest": "4165f2c157215872847f65036bab0f87", "start_type": "ATG", "rbs_motif": "AATAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3577.3857, "isoelectric_point": 4.0500284194946286 }, "id": "APKECPOBEN_636", "locus": "GCA002090215_00600" }, { "type": "cds", "sequence": "contig_1", "start": 340518, "stop": 341240, "strand": "+", "frame": 3, "gene": "hsdS", "product": "Putative type I specificity subunit S-MpnORF285P", "db_xrefs": [ "COG:COG0732", "COG:V", "EC:3.1.21.3", "GO:0003677", "GO:0009307", "KEGG:K01154", "SO:0001217", "UniRef:UniRef50_P75435", "UniRef:UniRef90_P75492" ], "nt": "TTGAAACTAGAAGCACCGAAATTCACAAACCGTGTTTTTAAAAATAGACCTAAGTTAACACATAAAACAATGGCCGAAATTCCCATTGATTTCCCACCCTTGAAAATTCAAGAGAAAATTGCTACTATCTTAGATACTTTTACCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGAGAGCGCAGGAAGCAGTATGCCTTCTATCGCGATTATCTGCTCAACCAAGAAAATATCCGAAAAATTTACGGAGCAAATATACCTTTCGAAACTTTTCAAATAAGGGATATTTGTGAAATAAATCGTGGTCGAGAAATAAATGAAAAATACCTTCGAGAAAATCCAGGTGAATTTCCAGTTTATTCCTCAGCAACAACAAATGGTGGTTTAATTGGAAAAATTAATGATTACGATTTTCATGGTGAATATGTTACTTGA", "aa": "MKLEAPKFTNRVFKNRPKLTHKTMAEIPIDFPPLKIQEKIATILDTFTELSAELSAELSAELSAELSAELSAELSAELSAELSAELSAELSAELSAELSAELSAELSAELSAELSAELSAELSAELSAELSAELSAELSAELSAELSAELSAELRERRKQYAFYRDYLLNQENIRKIYGANIPFETFQIRDICEINRGREINEKYLRENPGEFPVYSSATTNGGLIGKINDYDFHGEYVT", "aa_hexdigest": "1abc6e5c2ca6e49a68c9e368e0b3e7bb", "start_type": "TTG", "rbs_motif": "AAAA", "genes": [ "hsdS" ], "truncated": "both", "pseudogene": { "start": 340218, "stop": 341543, "observations": { "mutation": [ 340395, 341238 ], "stop": [ 340395, 341238 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75492", "query_cov": 1.0833333333333333, "subject_cov": 0.5273833671399595, "identity": 0.912, "score": 226.0, "evalue": 6.78e-68, "gene_start": 1, "gene_end": 240, "reference_start": 101, "reference_end": 360, "reference_sequence": "MEIKTYKIKDICDIKRGRVISKLDIKKDPGVFPVYSAATNNDGEFGRINSYDFDGEYVTWTADGYGGAVFYRNGKFSITNLCGLLKVKNKEISSKYLAHILKLEAPKFTNRVFKNRPKLTHKTMAEIPIDFPPLKIQEKIATILDTFTELSTELSTELSTELSTELSTELSTELSTELSTELSTELSTELSTELSTELSTELSTELSTELSTELSTELSTELSTELSTELSTELSTELSTELSTELSTELSTELSTELSTELSTELSAELSAELRERRKQYAFYRDYLLNQENIRKIYGANIPFETFQIRDICEINRGREINEKYLRENPGEFPVYSSATTNGGLIGKINDYDFHGEYVTWTTGGAHAGNVFYRNEKFSCSQNCGLLEVKNKNKFSSKFLCFALKLQSKKFVNYASAIPVLTIKRIAEIELSFPPLEIQEKIADILFAFEKLCNDLTEGIPAEIELRKKQLDYYQNFLFNWVQNKKLESLKSL" }, "paralog": false, "identity": 0.9457700650759219, "score": 622.0, "evalue": 9.59e-226, "description": "Internal stop codon at 340395, 341238. Nonsense mutation around 340395, 341238.", "psc": { "uniref90_id": "UniRef90_P75492", "gene": "HsdS", "product": "Putative type I specificity subunit S.MpnORF285P", "ec_ids": [ "3.1.21.3" ], "uniref50_id": "UniRef50_P75435", "cog_id": "COG0732", "cog_category": "V", "kegg_orthology_id": "K01154", "go_ids": [ "GO:0003677", "GO:0009307" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75492", "EC:3.1.21.3", "UniRef:UniRef50_P75435", "COG:COG0732", "COG:V", "KEGG:K01154", "GO:0003677", "GO:0009307" ] }, "pscc": { "uniref50_id": "UniRef50_P75435", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75435" ], "product": "Type I restriction enzyme MpnII specificity subunit" } }, "id": "APKECPOBEN_637", "locus": "GCA002090215_00601" }, { "type": "cds", "sequence": "contig_1", "start": 341313, "stop": 341600, "strand": "+", "frame": 3, "gene": null, "product": "restriction endonuclease subunit S", "db_xrefs": [ "EC:3.1.21.3", "KEGG:K01154", "SO:0001217", "UniRef:UniRef50_A0AB33HM83", "UniRef:UniRef90_UPI0019133825" ], "nt": "TTGTTAGAAGTAAAGAACAAAAATAAATTTAGTTCCAAATTCTTATGTTTTGCACTAAAACTACAATCTAAAAAATTTGTGAATTATGCCTCTGCAATTCCGGTTCTAACAATAAAGAGAATTGCAGAAATCGAACTCTCCTTTCCACCTCTAGAAATTCAAGAAAAGATTGCTGATATTTTGTTTGCATTTGAAAAACTTTGCAACGATTTAACCGAGGGGATTCCTGCCGAAATTGAATTGCGTAAAAAGCAATTGGATTACTATCAGAACTTCTTATTTAATTGA", "aa": "MLEVKNKNKFSSKFLCFALKLQSKKFVNYASAIPVLTIKRIAEIELSFPPLEIQEKIADILFAFEKLCNDLTEGIPAEIELRKKQLDYYQNFLFN", "aa_hexdigest": "a11db2ee396e898e6d3a89d9fdf70479", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 341013, "stop": 341609, "observations": { "mutation": [ 341238, 341598 ], "stop": [ 341238, 341598 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0019133825", "query_cov": 1.0, "subject_cov": 0.45454545454545453, "identity": 0.9890000000000001, "score": 184.0, "evalue": 1.83e-57, "gene_start": 1, "gene_end": 95, "reference_start": 108, "reference_end": 202, "reference_sequence": "KQYAFYRDYLLNQENIRKIYGANIPFETFQIRDICEINRGREINEKYLRENPGEFPVYSSATTNGGLIGKINDYDFHGEYVTWTTGGAHAGNVFYRNEKFSCSQNCGLLEVKNKNKFSSKFLCFALKLQSKKFVNYASAIPVLTIKRIAEIELSFPPLEIQEKIADILFAFEKLCNDLTEGIPAEIELRKKQLDYYQNFLFNWVQKIRN" }, "paralog": false, "identity": 0.98989898989899, "score": 397.0, "evalue": 9.02e-144, "description": "Internal stop codon at 341238, 341598. Nonsense mutation around 341238, 341598.", "psc": { "uniref90_id": "UniRef90_UPI0019133825", "product": "restriction endonuclease subunit S", "ec_ids": [ "3.1.21.3" ], "uniref50_id": "UniRef50_A0AB33HM83", "kegg_orthology_id": "K01154", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0019133825", "EC:3.1.21.3", "UniRef:UniRef50_A0AB33HM83", "KEGG:K01154" ] }, "pscc": { "uniref50_id": "UniRef50_A0AB33HM83", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB33HM83" ] } }, "id": "APKECPOBEN_638", "locus": "GCA002090215_00602" }, { "type": "cds", "sequence": "contig_1", "start": 341649, "stop": 341987, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W7Y3" ], "nt": "TTGGTCGAGGGTGGGTCATTTAGGAATATCTTTCCCCGTAGTCGTCGTCTGACTGCCACCCAGGGTGCCCTGGACATTCGCTTTGAGGCCAACGCCGACATTCGAAAAAGTGGATGCGGGTTCGGTTCCGGATGCATTTTGCGATTGTTGTCCCTGGGTTGTTACCCCACTCGTACTACTGTTGCTACCCCGGTGGTCATTCCCATATCTGTGCCCATTCCCAATCCAGGGAATATAATCCAACACCTGGTCGAGAACCGACCCCGCGAGGTTGAACCCGGTGGAGAAGCGCGTGAGCGTGGGGGAGAAGTGGGATCAGTTTGTGGTTTGCTGAACTAA", "aa": "MVEGGSFRNIFPRSRRLTATQGALDIRFEANADIRKSGCGFGSGCILRLLSLGCYPTRTTVATPVVIPISVPIPNPGNIIQHLVENRPREVEPGGEARERGGEVGSVCGLLN", "aa_hexdigest": "46bba2331b8592fbe389507071fc4d26", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0027E159D2", "query_cov": 1.0357142857142858, "subject_cov": 0.8226950354609929, "identity": 0.621, "score": 115.0, "evalue": 7.14e-31, "valid": false, "uniref50_id": "UniRef50_A0AB38W7Y3" }, "pscc": { "uniref50_id": "UniRef50_A0AB38W7Y3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W7Y3" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11958.60609999999, "isoelectric_point": 8.932562065124515 }, "id": "APKECPOBEN_639", "locus": "GCA002090215_00603" }, { "type": "cds", "sequence": "contig_1", "start": 342345, "stop": 342671, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTGGTGAATAAGAGTGGCCAAGATGATTCCAACAAGTTCCAAGCCACCGACCAAAAATGGTCTTACACCGAATTAAAGTCGGATCAAACCAAGCTCAACCTCCCCGCTTACGGGGAGGTGAATGGGTTGTTGAATCCGGCGTTGGTGGAGGTGTACGGTCTGAGTTCCACTCAAGGTTCTAGCACCGGAGCCGGTGGCGCTGGAGGTAACACCGGAGGCGACACCAATACCCAGACTTATGCACGACCCGGAATCGGCTTTAAATTACCCTCCACGGACTCGGAATCTTCCAAAGCCACCCTGATCACCCCCGGGTTGGCTTGA", "aa": "MLVNKSGQDDSNKFQATDQKWSYTELKSDQTKLNLPAYGEVNGLLNPALVEVYGLSSTQGSSTGAGGAGGNTGGDTNTQTYARPGIGFKLPSTDSESSKATLITPGLA", "aa_hexdigest": "865988431bf197728258f961b5f778c6", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11146.043099999984, "isoelectric_point": 4.850150871276854 }, "id": "APKECPOBEN_640", "locus": "GCA002090215_00604" }, { "type": "cds", "sequence": "contig_1", "start": 342895, "stop": 343383, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03257.18" ], "nt": "GTGGAGAGTGTGTGGGATTTCAAGGGGGTGTGGGCGGATCAAGCTCGGTCCGACTCGCAAGCAGCTACAAGTACCACCACCGCAACGAAGGCTACCTTATCGGAGCACACCAATGCTTTGGCCTTTCAGGTGAGTTATACCGACCAGGATTCGTACAAGGCTTCCACTCAAAGCTCCGGCCAAAACCAAAACACCTCCCCCTACCTGCACTTGGTGCAGGGGAAAAAAGTAGGTTCCTCTGATAAGCTTTCAGACGATCTTAAAAAACTGTTGGACCCCAACGAGGTTCGCGCCAAGCTGCGCCAAAGCTTTGGTACAGAAAATTCTACCCAACCCCAACCTAAAGCCCTCAAAACAATCACACCGGTATTTGGGACGAATAGTGGTAACCTCGGCAGTGTGCTTGGTGGTGGGGGTACCACGCAGGGCTCAGGCTCAGGTCAATCTGGTGTGGATCTCTCCCCCGTTGAACGGGTGAGTGGTCACTAA", "aa": "MESVWDFKGVWADQARSDSQAATSTTTATKATLSEHTNALAFQVSYTDQDSYKASTQSSGQNQNTSPYLHLVQGKKVGSSDKLSDDLKKLLDPNEVRAKLRQSFGTENSTQPQPKALKTITPVFGTNSGNLGSVLGGGGTTQGSGSGQSGVDLSPVERVSGH", "aa_hexdigest": "51f25bd1f003895c4c9fe521ee1a4337", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03257.18", "name": "Adhesin_P1_C", "length": 117, "aa_cov": 0.7098765432098766, "hmm_cov": 0.9914529914529915, "evalue": 3.2783713106419485e-47, "score": 155.24815368652344, "start": 48, "stop": 162 } ], "seq_stats": { "molecular_weight": 17037.35549999997, "isoelectric_point": 6.827408790588379 }, "id": "APKECPOBEN_641", "locus": "GCA002090215_00605" }, { "type": "cds", "sequence": "contig_1", "start": 343702, "stop": 344226, "strand": "+", "frame": 1, "gene": null, "product": "UPF0134 protein MPN_287", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002D866ED", "UniRef:UniRef100_P75490", "UniRef:UniRef50_P75490", "UniRef:UniRef90_P75490" ], "nt": "TTGCTGCCCACCTCTTTAAACACTTTTACGTTTTTCCAATTAATAATAAAGGAGGTCTTCACCATTAAACGCACAATTTCACTTACTTTGTTTAAGAAAAGACTCAACAAAGATAAGATTAATAATTGTCATGTTTGGGAAGAAGAGTTACCTGATGGTAGCTATGACATGGGATTTAGTGACAATTTAAACCATATGGAAAAACGAAAAAGTGGTTATGTTACCCAAAAGCAGTTTAGCGAGTTCAAAGATGCCAATAATCAAAGGCTGATAAAGATTGAAACTACTTTAGCTACTCAAGGTGAACAATTAAATCAATTAATTAAAGTTGTTATTCTTCAGGGCGAACAAATTAAAGAACTTCAAGTGGAGCAAAAAGCTCAAGGAGAACAAATAAAAGCTAAAGGAGAACAAATAAAAGCTCAAAGCGAGCAAATAAAAACTCAAGGAGAAACGCTTAAATTGATCCTACAAGCACTTGGAGGAATAAATAAGCGCTTAGACAAAGTTGATCCGCCTAAATAG", "aa": "MLPTSLNTFTFFQLIIKEVFTIKRTISLTLFKKRLNKDKINNCHVWEEELPDGSYDMGFSDNLNHMEKRKSGYVTQKQFSEFKDANNQRLIKIETTLATQGEQLNQLIKVVILQGEQIKELQVEQKAQGEQIKAKGEQIKAQSEQIKTQGETLKLILQALGGINKRLDKVDPPK", "aa_hexdigest": "4f27146806afe1ad4690dfc75aa0b72c", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0002D866ED", "uniref100_id": "UniRef100_P75490", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002D866ED", "UniRef:UniRef100_P75490" ] }, "ips": { "uniref100_id": "UniRef100_P75490", "uniref90_id": "UniRef90_P75490" }, "psc": { "uniref90_id": "UniRef90_P75490", "product": "UPF0134 protein MPN_287", "uniref50_id": "UniRef50_P75490" }, "pscc": { "uniref50_id": "UniRef50_P75490", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75490" ], "product": "UPF0134 protein MPN_287" }, "genes": [], "id": "APKECPOBEN_642", "locus": "GCA002090215_00606" }, { "type": "cds", "sequence": "contig_1", "start": 344822, "stop": 345463, "strand": "+", "frame": 2, "gene": null, "product": "P68 family surface lipoprotein", "db_xrefs": [ "GO:0009897", "SO:0001217", "UniRef:UniRef50_P75595", "UniRef:UniRef90_A0AB38WA11" ], "nt": "ATGAAATTTAAGTATTGTGCCATCTTTTTCAGTGGCTTTCTAGGTCTTTCTGCCATCTTAGCTGCTTGTGGAGCGCGGGGCAAATTTGATCAAGTTGATGATGGCAAGATTAAATTGGCTTTCTCTTTAACTTCAAAAAGTGCATCAAACGCCTTACAAGCAATTGTTAAAAAATATAACGAAGTTAAAAAACCTGGTGATTACCATATTGAAATTACCCAAATCGCTGGTGGTTATGATGGTGGTCGTAGCGATTTACAAACCCGAGTCAACGTTAAGGACACCACTAACTTTTACAACCTTATCTTGAATTATCCTGACTTAGTTTCTACTTTAGGTCGTGTTGGGATGGAACTACTGTTTGATAAAGTTAATACAGATAAATTATCCGACCGTTTCTTAGATTTCAATAAACGAATTAGTGCAATTTCTAAACCAGGGATTTACGGTATTCCGGTTTCTTTATCCACCGAAGTTTTATCGATCAACGGACCGGTGTTGCACTATATTTTGAAAAGTGCAAAAGGTGATAGTGACAATGTTAAAGTAAGTCAAAGGACTGGAGAAACTACTCAAAAAAGTAAAGTAACTAATCCTTTGAAAATTAATACTCAAAACGATCCTGCTACAAAAGATCTTTGA", "aa": "MKFKYCAIFFSGFLGLSAILAACGARGKFDQVDDGKIKLAFSLTSKSASNALQAIVKKYNEVKKPGDYHIEITQIAGGYDGGRSDLQTRVNVKDTTNFYNLILNYPDLVSTLGRVGMELLFDKVNTDKLSDRFLDFNKRISAISKPGIYGIPVSLSTEVLSINGPVLHYILKSAKGDSDNVKVSQRTGETTQKSKVTNPLKINTQNDPATKDL", "aa_hexdigest": "43c47b40f91aac03cb97426358e1305f", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 344822, "stop": 345766, "observations": { "mutation": [ 345461, 345641, 345710 ], "stop": [ 345461, 345641, 345710 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB38WA11", "query_cov": 1.0, "subject_cov": 0.44375, "identity": 1.0, "score": 415.0, "evalue": 1.13e-142, "gene_start": 1, "gene_end": 213, "reference_start": 1, "reference_end": 213, "reference_sequence": "MKFKYCAIFFSGFLGLSAILAACGARGKFDQVDDGKIKLAFSLTSKSASNALQAIVKKYNEVKKPGDYHIEITQIAGGYDGGRSDLQTRVNVKDTTNFYNLILNYPDLVSTLGRVGMELLFDKVNTDKLSDRFLDFNKRISAISKPGIYGIPVSLSTEVLSINGPVLHYILKSAKGDSDNVKVSQRTGETTQKSKVTNPLKINTQNDPATKDLWEKIEASAKANGKSNKDGQTKGKKKVEKSSSSSLVNLKQSTDQTTTDDGSQKSDNKIKESWGEYQEVDGGLKNFEFKASIFENWHDLLDFSTRVAKSFTNVKGKDIKKGTDIQGVLGVDSTPNSLFTSVFAAGGGDYNNFFYKIENGRADFSNFKNKGTSYQNLQKVFGDFKGLIDKNGIFVNKGGSYSSNFQKFHQLAYSISSTSGFFYSFAGNSAKRLKFGDNFIEFPRFTQEIKAPSTENGGQSNLLGTFEVKDSSKSKEVKKN" }, "paralog": false, "identity": 0.9904458598726115, "score": 606.0, "evalue": 5.13e-220, "description": "Internal stop codon at 345461, 345641, 345710. Nonsense mutation around 345461, 345641, 345710.", "psc": { "uniref90_id": "UniRef90_A0AB38WA11", "product": "P68 family surface lipoprotein", "uniref50_id": "UniRef50_P75595", "go_ids": [ "GO:0009897" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB38WA11", "UniRef:UniRef50_P75595", "GO:0009897" ] }, "pscc": { "uniref50_id": "UniRef50_P75595", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75595" ], "product": "Uncharacterized lipoprotein MPN_097" } }, "id": "APKECPOBEN_643", "locus": "GCA002090215_00607" }, { "type": "cds", "sequence": "contig_1", "start": 345740, "stop": 346822, "strand": "+", "frame": 2, "gene": null, "product": "P68 family surface lipoprotein", "db_xrefs": [ "GO:0005886", "GO:0009897", "SO:0001217", "UniRef:UniRef50_P75280", "UniRef:UniRef90_P75489" ], "nt": "GTGGCTAAGTCGTTTACAAATGTAAAGGGAAAAGATATCAAAAAGGGAACTGATATCCAAGGTGTACTTGGTGTTGACAGTACGCCCAACTCATTATTCACCTCTGTTTTTGCAGCTGGTGGCGGTGATTACAATAATTTCTTCTACAAAATTGAAAACGGCCGTGCTGACTTTAGTAACTTTAAGAATAAAGGCACTTCCTACCAAAATCTTCAAAAAGTATTTGGTGATTTCAAAGGCTTAATTGACAAAAATGGTATCTTTGTGAATAAGGGTGGATCTTACTCATCAAACTTTCAAAAGTTCCATCAATTAGCTTACAGTATTTCCTCTACTTCCGGTTTTTTCTATTCGTTTGCCGGTAACAGCGCTAAACGCTTAAAGTTTGGTGATAACTTTATTGAATTTCCTCGTTTTACTCAGGAAATTAAAGCACCTTCTACTGAAAATGGCGGCCAAAGCAATCTTTTAGGAACCTTTGAAGTTAAAGATAGCAGTAAATCTAAGGAAGTGAAAAAAACTAATAGGAAAGAAGATGGTGCTCAAAATCAAGGTAAAAAAGAAAGCGATAAGAAAACCATTTACCTTTACAAAAGTCAAATTCCATCAGACAAAACAGAAGGCGAAAATGCCATCTTAATCAAAGAACAAAACGTTATTAATCAGCTAGAACAAGCAGCTAAAAAAGATGAAAAAGGAGAGACTGTTACAAACAAAGTTGCATCATTAGAAACCAAAGCTGCTAATGCCAAAAAGGATAGTAGTAAAACCATTATTGGTTACACCACAACTGATAATGTTCATGAAGACGGTAAAAACATTTTCAAAATTAACAAACTCAAAACTGAAGACTATGATCGTAAGATTATTGTCGGTGCCACTGAAGAAGTGTTAGAGCAATCTAGCACATTACAAAGTGATGAAGCAATTGTGTTACCAGCTCCTGGTAAGTATCAAAGTGGTGACGCTAAGAAAGTAACCATCACCCAAGGTCCTAATATCATTGGCATTCACGCTAACGAAAAGGAAAACGCCGAAACGCAAAAGTTTGTTAACTGGTTTTTAAACAATACAGAAAGTTGA", "aa": "MAKSFTNVKGKDIKKGTDIQGVLGVDSTPNSLFTSVFAAGGGDYNNFFYKIENGRADFSNFKNKGTSYQNLQKVFGDFKGLIDKNGIFVNKGGSYSSNFQKFHQLAYSISSTSGFFYSFAGNSAKRLKFGDNFIEFPRFTQEIKAPSTENGGQSNLLGTFEVKDSSKSKEVKKTNRKEDGAQNQGKKESDKKTIYLYKSQIPSDKTEGENAILIKEQNVINQLEQAAKKDEKGETVTNKVASLETKAANAKKDSSKTIIGYTTTDNVHEDGKNIFKINKLKTEDYDRKIIVGATEEVLEQSSTLQSDEAIVLPAPGKYQSGDAKKVTITQGPNIIGIHANEKENAETQKFVNWFLNNTES", "aa_hexdigest": "821b452638e11938494d378b5c1fb560", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 345440, "stop": 347125, "observations": { "mutation": [ 345461, 345641, 345710, 346820 ], "stop": [ 345461, 345641, 345710, 346820 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75489", "query_cov": 1.0, "subject_cov": 0.4235294117647059, "identity": 0.997, "score": 686.0, "evalue": 2.9e-241, "gene_start": 1, "gene_end": 360, "reference_start": 370, "reference_end": 729, "reference_sequence": "MKFKYCAIFFSGFLGLSAILAACGTKGKFDQVDDGKIKLASSLTSKSASKALQAIVKKYNEVKKPGDYPIEITQIAGGYDGGRSDLQTRVNVKDTTNFYNLILNYPDLVSTLGRVGMELLFDKVNTDKLSDRFLDFNKRISAISKPGIYGIPVSLSTEVLSINGPVLHYILKSAKGDSDNVKVSQRTGETTQKDRFLDFNNRISAISKPGIYGIPVSLSTEVLSINGPVLHYILKSAKGDSDNVKVSQRTGETTQKSKVTNPLKINTQNDPATKDLWEKIEASAKANGKSNKDGQTKGKKKVEKSSSSSLVNLKQSTDQTTTDDGSQKSDNKIKESWGEYQEVDGGLKNFEFKASIFENWHDLLDFSTRVAKSFTNVKGKDIKKGTDIQGVLGVDSTPNSLFTSVFAAGGGDYNNFFYKIENGRADFSNFKNKGTSYQNLQKVFGDFKGLIDKNGIFVNKGGSYSSNFQKFHQLAYSISSTSGFFYSFAGNSAKRLKFGDNFIEFPRFTQEIKAPSTENGGQSNLLGTFEVKDSSKSKEVKKTNRKEDGAQNQGKKESDKKTIYLYKSQIPSDKTEGENAILIKEQNVINQLEQAAKKDEKGETVTNKVASLETKAANAKKDSSKTIIGYTTTDNVHEDGKNIFKINKLKTEDYDRKIIVGATEEVLEQSSTLQSDEAIVLPAPGKYQSGDAKKVTITQGPNIIGIHANEKENAETQKFVNWFLNNTESWEVKNGKDSSTKQQTAAEFFAESASYILPLKEIFDKDNKKATENKDNNTSNKKQANTYAEKALDLFQQISKGDIVSYSDPSDFRSGKFRDGIGSNFNAAVSSKADFDRFVKGFIATLGSEI" }, "paralog": false, "identity": 0.9928698752228164, "score": 1092.0, "evalue": 0.0, "description": "Internal stop codon at 345461, 345641, 345710, 346820. Nonsense mutation around 345461, 345641, 345710, 346820.", "psc": { "uniref90_id": "UniRef90_P75489", "product": "P68 family surface lipoprotein", "uniref50_id": "UniRef50_P75280", "go_ids": [ "GO:0005886", "GO:0009897" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75489", "UniRef:UniRef50_P75280", "GO:0005886", "GO:0009897" ] }, "pscc": { "uniref50_id": "UniRef50_P75280", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75280" ], "product": "Uncharacterized lipoprotein MPN_506" } }, "id": "APKECPOBEN_644", "locus": "GCA002090215_00608" }, { "type": "cds", "sequence": "contig_1", "start": 346901, "stop": 347185, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCTTTAAAAGAAATCTTTGACAAAGATAACAAAAAAGCTACTGAAAATAAGGACAATAATACTAGCAATAAAAAACAAGCTAATACCTATGCTGAAAAGGCGTTAGATTTGTTCCAACAAATTAGTAAAGGTGATATAGTTAGTTACAGCGACCCTAGCGATTTTAGAAGTGGTAAATTCCGCGATGGGATTGGCTCTAATTTTAACGCTGCAGTAAGTTCCAAAGCTGATTTTGACAGGTTTGTTAAAGGCTTCATCGCCACTTTGGGTAGTGAAATTTAA", "aa": "MPLKEIFDKDNKKATENKDNNTSNKKQANTYAEKALDLFQQISKGDIVSYSDPSDFRSGKFRDGIGSNFNAAVSSKADFDRFVKGFIATLGSEI", "aa_hexdigest": "ccda41382d0f2345a6eb88c671150457", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10433.430199999992, "isoelectric_point": 7.908417320251465 }, "id": "APKECPOBEN_645", "locus": "GCA002090215_00609" }, { "type": "cds", "sequence": "contig_1", "start": 347275, "stop": 347454, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01420.25" ], "nt": "GTGCAAATCAAAACTTACAAAATCAAAGATATTTGCGATATTAAACGCGGTAGAGTTATCAGCAAACTATATATCAAAAACAATCCAGGCGAATTCCCTGTTTATTCATCGGCTACAGTTAATAATGGTGAAATTGGCAGAATAAAAGATTGTGATCTTAAGGGTGAGTATGTGACTTGA", "aa": "MQIKTYKIKDICDIKRGRVISKLYIKNNPGEFPVYSSATVNNGEIGRIKDCDLKGEYVT", "aa_hexdigest": "586c91d0b4398bcdcba1cdfcc9a437c9", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01420.25", "name": "Methylase_S", "length": 60, "aa_cov": 0.9491525423728814, "hmm_cov": 0.9833333333333333, "evalue": 7.732049989098151e-11, "score": 37.9355354309082, "start": 4, "stop": 59 } ], "seq_stats": { "molecular_weight": 6724.738800000003, "isoelectric_point": 9.230019187927244 }, "id": "APKECPOBEN_646", "locus": "GCA002090215_00610" }, { "type": "cds", "sequence": "contig_1", "start": 347632, "stop": 347820, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAGGAACTTTAGCAGAAATTGAGCTAGATTTCACAAGCAAGAAAATTCAAGAGAAAATTGCTACTATCTTAGATACTTTTACCGAGCTAAGCGCCGAGCTAAGCGCCGAGCTAAGCGCCGAGCTAAGAGAGCGAAAGAAGCAGTATGTCTTCTATAGCGATTATTTACTTAACCCAAAAAATTGA", "aa": "MQGTLAEIELDFTSKKIQEKIATILDTFTELSAELSAELSAELRERKKQYVFYSDYLLNPKN", "aa_hexdigest": "9bdbf408be85383351fff88d1f4d85ea", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7173.0662999999995, "isoelectric_point": 4.793937110900879 }, "id": "APKECPOBEN_647", "locus": "GCA002090215_00611" }, { "type": "cds", "sequence": "contig_1", "start": 347965, "stop": 348357, "strand": "+", "frame": 1, "gene": null, "product": "Putative type I specificity subunit S-MpnORF289P C-terminus", "db_xrefs": [ "EC:3.1.21.3", "GO:0003677", "GO:0009307", "KEGG:K01154", "SO:0001217", "UniRef:UniRef50_P75487", "UniRef:UniRef90_P75487" ], "nt": "TTGGTTTACAGTAAAACTTTTCGAGTTGAAGAAAAATCCATTACGGTAAGTGCTAGAGGCACAATTGGTGTCGTTTTCTATCGTGATTTTGCTTATTTGCCTGCTGTTAGTTTAATTTGCTTTGTCCCTAAAGAAGAATTTGATATAAGGTTCTTATTCCACGCATTAAGAGCCATTAAATTTAAAAAGCAAGGCTCTGCAACAGGCCAGTTAACAGTGGCACAATTTAAGGAATATGGTATTCATGTACCTTCGCTGAAAAAACAGAAAGAAATCGCCGCTATCTTAGATCCACTTTACAGCTTTTTTACTGATCTAAACGAAGGTCTTCCTGCTGAAATTGAATTGCGTAAAAAGCAATTGGATTACTATCAGAACTTTTTATTTAACTGA", "aa": "MVYSKTFRVEEKSITVSARGTIGVVFYRDFAYLPAVSLICFVPKEEFDIRFLFHALRAIKFKKQGSATGQLTVAQFKEYGIHVPSLKKQKEIAAILDPLYSFFTDLNEGLPAEIELRKKQLDYYQNFLFN", "aa_hexdigest": "fb11dd98dd00bd77a5f315adf3f2804a", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75487", "query_cov": 1.0, "subject_cov": 0.896551724137931, "identity": 1.0, "score": 258.0, "evalue": 3.85e-87, "valid": true, "product": "Putative type I specificity subunit S.MpnORF289P C-terminus", "ec_ids": [ "3.1.21.3" ], "uniref50_id": "UniRef50_P75487", "kegg_orthology_id": "K01154", "go_ids": [ "GO:0003677", "GO:0009307" ] }, "pscc": { "uniref50_id": "UniRef50_P75487", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75487" ], "product": "Putative type I specificity subunit S.MpnORF289P C-terminus" }, "genes": [], "id": "APKECPOBEN_648", "locus": "GCA002090215_00612" }, { "type": "cds", "sequence": "contig_1", "start": 348395, "stop": 348985, "strand": "-", "frame": 3, "gene": "tsaB", "product": "tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex dimerization subunit type 1 TsaB", "db_xrefs": [ "COG:COG1214", "COG:J", "EC:2.3.1.234", "GO:0002949", "RefSeq:WP_014325445.1", "SO:0001217", "UniParc:UPI0001DD651C", "UniRef:UniRef100_A0AAP8E4Y3", "UniRef:UniRef50_P47450", "UniRef:UniRef90_P75486" ], "nt": "ATGCGCTTTTTCAATAAGTACAAGCTCTTTTTGGACTGTGCTTACAAACACCTCAATATCGTACTGTTGGATTTTAAAACGAATACTGTGGTGGATCAACTAACAATACCAGTACAACAAAACCTCACGGAGTTGGCTGTGTATCACTTGGAAAAATTGTTAAAGAAAAACAAAGTACGCAATAACACTGTTAGACAGTTTTACGTAACCACTGGACCGGGTAGCTTTACTGGTCAACGCGTTGGTGCCATTATTGCTAAAACTTGGTGCACAGTAAACCCTAACTGTCAACTCTTTGCTTTAAACTCACTCCGCCTTCAAATTCCTTATGGCTGTGGCATTAGCAAAATTAGTGCTGGCAATGAAAAGAACTACTGTGGTTTGTTTACCGAAACTACGAGTGAAATAGCACTCTTAGCTAAACCAGATTTTGTCAAGCTGTGCAAAGCAAATACCGAGTTACCGTTATATGAAAACTTTGAAAACATCGACAGTATTGAGGAGTTATTCCTAAACAACATCGAACGGTTTGAACTAGTGGAAAATCCCCAAAATTTAGAACTACTCTACCTTAAAGATCCCGTTAATTAG", "aa": "MRFFNKYKLFLDCAYKHLNIVLLDFKTNTVVDQLTIPVQQNLTELAVYHLEKLLKKNKVRNNTVRQFYVTTGPGSFTGQRVGAIIAKTWCTVNPNCQLFALNSLRLQIPYGCGISKISAGNEKNYCGLFTETTSEIALLAKPDFVKLCKANTELPLYENFENIDSIEELFLNNIERFELVENPQNLELLYLKDPVN", "aa_hexdigest": "4637762e47aa82473716d0c7947bb369", "start_type": "ATG", "rbs_motif": "AAAA", "ups": { "uniparc_id": "UPI0001DD651C", "ncbi_nrp_id": "WP_014325445.1", "uniref100_id": "UniRef100_A0AAP8E4Y3", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD651C", "RefSeq:WP_014325445.1", "UniRef:UniRef100_A0AAP8E4Y3" ] }, "ips": { "uniref100_id": "UniRef100_A0AAP8E4Y3", "uniref90_id": "UniRef90_P75486" }, "psc": { "uniref90_id": "UniRef90_P75486", "gene": "tsaB", "product": "tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex dimerization subunit type 1 TsaB", "ec_ids": [ "2.3.1.234" ], "uniref50_id": "UniRef50_P47450", "cog_id": "COG1214", "cog_category": "J", "go_ids": [ "GO:0002949" ] }, "pscc": { "uniref50_id": "UniRef50_P47450", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47450" ], "product": "Uncharacterized protein MG208" }, "genes": [ "tsaB" ], "id": "APKECPOBEN_649", "locus": "GCA002090215_00613" }, { "type": "cds", "sequence": "contig_1", "start": 348969, "stop": 349319, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGGGAACAGTTCTAACAAGGCCAAGAATGCTTTTACTAAGTTTACGGTGTTAAACCAAAACGAAAAGGCTGCTTTAATTAAGTGTGAACTGTTAACCGGACGAACTCACCAAATTAGGGTACACTTGCAGTTTATTAAACACCCCGTTTACAACGATCCCTTATACGGTTTGAAGAGTGAGCAAGCTACTGAATATGGCCAGTATTTACACGCGCAACAAATTAGTTTTATCCACCCCACTTTGAATAAGGAAATGGGTTTTGAAGCACAGTTAGATAAAACTTTTAGCGATAAGTTAGATAATTTAAACTTGAAAATTGCTAATTCCCTTTATGCGCTTTTTCAATAA", "aa": "MGNSSNKAKNAFTKFTVLNQNEKAALIKCELLTGRTHQIRVHLQFIKHPVYNDPLYGLKSEQATEYGQYLHAQQISFIHPTLNKEMGFEAQLDKTFSDKLDNLNLKIANSLYALFQ", "aa_hexdigest": "5327af54ee972bd120c23b973a9ed1d7", "start_type": "GTG", "rbs_motif": "AATAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13287.015299999986, "isoelectric_point": 9.015726280212402 }, "id": "APKECPOBEN_650", "locus": "GCA002090215_00614" }, { "type": "cds", "sequence": "contig_1", "start": 349323, "stop": 349748, "strand": "-", "frame": 2, "gene": "rluA", "product": "putative RNA pseudouridine synthase MG209-like protein", "db_xrefs": [ "COG:COG0564", "COG:J", "EC:5.4.99.-", "GO:0000455", "GO:0003723", "GO:0120159", "KEGG:K06180", "SO:0001217", "UniRef:UniRef50_P47451", "UniRef:UniRef90_P75485" ], "nt": "GTGCAACCAGAAGACCGGGTACACGTTAATTGGAGTGAGGAACTGTTTGAAAAAGTGCCAGTTGAAGTCCAGCCCTATGACTTTCCCTTGGACATCTTATATGAAGATGAGCAAATTATGGTGGTCAATAAACCCAATGGTTTAATTAGCCACCCGACTAGTTTTAATGAAAGTGAAAGTTTACTGGGTGCCGCTTTGTTTCACTGCAACCACCAACCGGTGTTTTTAGTCCACCGCCTAGACCGTGATACGAGTGGTGTGATTATGCTAGCGAAAAACCAAAGTAGCTTGCTGCACTTGCAAAAACAGCTCCAGCAACGGGTTATGAAGCGTTATTACTTAGCTTTAGTCCATTTTCCCCTAGACAGTTTGTCTGGTACCATTAGCGCCCCGTTAGAACGAGTGGGTAACAATAAGGTCATGTGA", "aa": "MQPEDRVHVNWSEELFEKVPVEVQPYDFPLDILYEDEQIMVVNKPNGLISHPTSFNESESLLGAALFHCNHQPVFLVHRLDRDTSGVIMLAKNQSSLLHLQKQLQQRVMKRYYLALVHFPLDSLSGTISAPLERVGNNKVM", "aa_hexdigest": "f4b07f53c49b25a332940f18f3a24ad4", "start_type": "GTG", "rbs_motif": null, "genes": [ "rluA" ], "truncated": "both", "pseudogene": { "start": 349020, "stop": 349898, "observations": { "mutation": [ 349325, 349754 ], "stop": [ 349325, 349754 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75485", "query_cov": 1.0, "subject_cov": 0.45483870967741935, "identity": 0.993, "score": 282.0, "evalue": 5.52e-94, "gene_start": 1, "gene_end": 141, "reference_start": 51, "reference_end": 191, "reference_sequence": "MEQTFSVTTAQRLDTFLATLLNLSRVKVAKLIVDGLVSVNGKKITKNGWLVQPEDRVHVNWSEELFEKVPVEVQPYDFPLDILYEDEQIMVVNKPNGLISHPTSFNESESLLGAALFHCNHQPVFLVHRLDRDTSGVIMLAKNQSSLLHLQKQLQQRVMKRYYLALVHFPLDSLSGTISAPLERVGNNKVMWKVGNSSNKAKNAFTKFTVLNQNEKAALIKXCELLTGRTHQIRVHLQFIKHPVYNDPLYGLKSEQATEYGQYLHAQQISFIHPTLNKEMGFEAQLDKTFSDKLDNLNLKIANSLYALFQ" }, "paralog": false, "identity": 0.9897610921501706, "score": 570.0, "evalue": 8.82e-210, "description": "Internal stop codon at 349325, 349754. Nonsense mutation around 349325, 349754.", "psc": { "uniref90_id": "UniRef90_P75485", "gene": "RluA", "product": "Uncharacterized RNA pseudouridine synthase MG209 homolog", "ec_ids": [ "5.4.99.-" ], "uniref50_id": "UniRef50_P47451", "cog_id": "COG0564", "cog_category": "J", "kegg_orthology_id": "K06180", "go_ids": [ "GO:0003723", "GO:0120159", "GO:0000455" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75485", "EC:5.4.99.-", "UniRef:UniRef50_P47451", "COG:COG0564", "COG:J", "KEGG:K06180", "GO:0003723", "GO:0120159", "GO:0000455" ] }, "pscc": { "uniref50_id": "UniRef50_P47451", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47451" ], "product": "Uncharacterized RNA pseudouridine synthase MG209" } }, "id": "APKECPOBEN_651", "locus": "GCA002090215_00615" }, { "type": "cds", "sequence": "contig_1", "start": 349752, "stop": 349898, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01479.30" ], "nt": "ATGGAACAAACTTTTAGTGTTACTACAGCCCAACGCTTAGATACCTTTTTGGCCACACTACTCAACCTTTCCCGGGTTAAAGTAGCCAAGTTAATTGTCGATGGTTTGGTGAGTGTTAACGGTAAAAAAATTACTAAAAATGGTTGA", "aa": "MEQTFSVTTAQRLDTFLATLLNLSRVKVAKLIVDGLVSVNGKKITKNG", "aa_hexdigest": "611db4e9f4b8d7087542eda5e7a7ab1c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01479.30", "name": "S4", "length": 38, "aa_cov": 0.7708333333333334, "hmm_cov": 1.0, "evalue": 9.14990130995376e-12, "score": 40.05647277832031, "start": 11, "stop": 47 } ], "seq_stats": { "molecular_weight": 5220.094100000002, "isoelectric_point": 10.165906715393067 }, "id": "APKECPOBEN_652", "locus": "GCA002090215_00616" }, { "type": "cds", "sequence": "contig_1", "start": 349898, "stop": 349999, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTTAACTTAGCGGACTGTTGCATTACCTTTAGTTTTATTGGTCTGTTCCTCTCCTTTTTAATTCAGTTCTTCAAAGAAATGAAGCAAACTAAATCTTAA", "aa": "MFNLADCCITFSFIGLFLSFLIQFFKEMKQTKS", "aa_hexdigest": "54ad894d5bc253b4bf89f0e5464f84d7", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3899.661100000001, "isoelectric_point": 7.799981498718263 }, "id": "APKECPOBEN_653", "locus": "GCA002090215_00617" }, { "type": "cds", "sequence": "contig_1", "start": 350102, "stop": 350452, "strand": "-", "frame": 3, "gene": "lspA", "product": "signal peptidase II", "db_xrefs": [ "COG:COG0597", "COG:MU", "EC:3.4.23.36", "GO:0004190", "GO:0005886", "GO:0006508", "GO:0009306", "KEGG:K03101", "SO:0001217", "UniRef:UniRef50_P75484", "UniRef:UniRef90_P75484" ], "nt": "ATGGCAAAAGCCCCAACTTTTTTTTCAAAATTGCTAAAGCAAATCCTTTTTGCTAACCGCAAACCCTTTTTGTACTATAAGCTTGCCTTAATCCTGTTTGTCGGTTTTGTCATTTTGTTTCAGGTATTTATGCTGCGTGCTGCCTTAAATGGTGAGAAGGGTATTAATGGTGCTAATGGTACAGATGTAGCTCGCAGTTCCTTTATTAGTATCTATGTTATCGGTAACAAGGGGGTTGGTTTTAGCTTATTGGCAGACCAACCAGGACTTGTTTACTTTCTCCAGGGTTTTCTATCTTTCATAGCACTGTTCTTTTTGGTTTTCTCTACTAGTTACAACTACATCTTTTGA", "aa": "MAKAPTFFSKLLKQILFANRKPFLYYKLALILFVGFVILFQVFMLRAALNGEKGINGANGTDVARSSFISIYVIGNKGVGFSLLADQPGLVYFLQGFLSFIALFFLVFSTSYNYIF", "aa_hexdigest": "a2ce7117ea06a98a81dda82195433baf", "start_type": "ATG", "rbs_motif": "AAAA", "genes": [ "lspA" ], "truncated": "5-prime", "pseudogene": { "start": 349898, "stop": 350452, "observations": { "mutation": [ 350104 ], "stop": [ 350104 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75484", "query_cov": 1.0, "subject_cov": 0.6304347826086957, "identity": 1.0, "score": 217.0, "evalue": 2.3e-70, "gene_start": 1, "gene_end": 116, "reference_start": 1, "reference_end": 116, "reference_sequence": "MAKAPTFFSKLLKQILFANRKPFLYYKLALILFVGFVILFQVFMLRAALNGEKGINGANGTDVARSSFISIYVIGNKGVGFSLLADQPGLVYFLQGFLSFIALFFLVFSTSYNYIFWITTLAFGSLGNFFDRLTSGSGEVLDYFVFSGGNSVFNLADCCITFSFIGLFLSFLIQFFKEMKQTKS" }, "paralog": false, "identity": 0.9945652173913043, "score": 359.0, "evalue": 6.79e-129, "description": "Internal stop codon at 350104. Nonsense mutation around 350104.", "psc": { "uniref90_id": "UniRef90_P75484", "gene": "lspA", "product": "signal peptidase II", "ec_ids": [ "3.4.23.36" ], "uniref50_id": "UniRef50_P75484", "cog_id": "COG0597", "cog_category": "MU", "kegg_orthology_id": "K03101", "go_ids": [ "GO:0004190", "GO:0005886", "GO:0006508", "GO:0009306" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75484", "EC:3.4.23.36", "UniRef:UniRef50_P75484", "COG:COG0597", "COG:MU", "KEGG:K03101", "GO:0004190", "GO:0005886", "GO:0006508", "GO:0009306" ] }, "pscc": { "uniref50_id": "UniRef50_P75484", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75484" ], "product": "Lipoprotein signal peptidase" } }, "id": "APKECPOBEN_654", "locus": "GCA002090215_00618" }, { "type": "cds", "sequence": "contig_1", "start": 350491, "stop": 351111, "strand": "+", "frame": 1, "gene": "yajL", "product": "putative protein MPN_294", "db_xrefs": [ "COG:COG0693", "COG:V", "RefSeq:WP_010874651.1", "SO:0001217", "UniParc:UPI000013A574", "UniRef:UniRef100_P75483", "UniRef:UniRef50_P75483", "UniRef:UniRef90_P75483" ], "nt": "ATGGATTTGCTTACAACCGCAAACAAAACGCCGCGAATCGCCAAATCACGTCCTGTTCGAATTGCCATCATTATAACGCAGAAGACTAATGATCTGCACGCTACTGTACCCTGCTTTTTATGGCGCAAAGCAAGGTATGCTGTTGACCTGATTTCCGCTGAAGCCAAAGCTAGCATTATGTTAGAGATGGGGATTCATGTGCGCTGTGACAACACTTTGTCCAAAACTAACTTTAACCAGTATACCGCTGCTTTTATTCCCCATGGCAACACTACTAGGTTGGTGGAAATAGACAAACTAAGAAAGGACTTGGAGAAGTTTGTCTATAAACCCAAGGGACCAATGCGCTGGTTGTTTAGCAGTGGTAATGGTGCTTGTGTTTTGAAGGAGTTTGACTTGATTGCCCCTGACCAATTAGTCACCGTTCAAAACGAAAAGGAAATGGTTAAACTTTTGGGAAAAAATTTCATCAAGCAACCAGTACATGTGGACAAAAACATTATTAGTTGTGCCAATTCTTGTGGCTTGACTAAATTCTCCTTTAAGGTAATTGAAGAGCTTTCAGGAATAGAACTAGCGCGCAAAACGGCGAATCTTGTTGACCATATCTACAAAGGATAA", "aa": "MDLLTTANKTPRIAKSRPVRIAIIITQKTNDLHATVPCFLWRKARYAVDLISAEAKASIMLEMGIHVRCDNTLSKTNFNQYTAAFIPHGNTTRLVEIDKLRKDLEKFVYKPKGPMRWLFSSGNGACVLKEFDLIAPDQLVTVQNEKEMVKLLGKNFIKQPVHVDKNIISCANSCGLTKFSFKVIEELSGIELARKTANLVDHIYKG", "aa_hexdigest": "8eb1170c0b77bc3c543ae945ca8b91cc", "start_type": "ATG", "rbs_motif": "AAAA", "ups": { "uniparc_id": "UPI000013A574", "ncbi_nrp_id": "WP_010874651.1", "uniref100_id": "UniRef100_P75483", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013A574", "RefSeq:WP_010874651.1", "UniRef:UniRef100_P75483" ] }, "ips": { "uniref100_id": "UniRef100_P75483", "uniref90_id": "UniRef90_P75483" }, "psc": { "uniref90_id": "UniRef90_P75483", "gene": "YajL", "product": "Uncharacterized protein MPN_294", "uniref50_id": "UniRef50_P75483", "cog_id": "COG0693", "cog_category": "V" }, "pscc": { "uniref50_id": "UniRef50_P75483", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75483" ], "product": "Uncharacterized protein MPN_294" }, "genes": [ "yajL" ], "id": "APKECPOBEN_655", "locus": "GCA002090215_00619" }, { "type": "cds", "sequence": "contig_1", "start": 351373, "stop": 351519, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTCACTAAGCAAATTAAAGCTGGCAGTGTTAACGAAGTGTTAGCGCAAAAATTAGTCCAACTCGATGCGCTGCGCACTAACTATCTCATTGGCGCTTTGTACTCCACCAATAAAACACAAAAGAAGCTGTTTAAGCAATCGTGA", "aa": "MFTKQIKAGSVNEVLAQKLVQLDALRTNYLIGALYSTNKTQKKLFKQS", "aa_hexdigest": "9baa1dc809fb450e3e5ff1f7f9ef57b2", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5430.325400000001, "isoelectric_point": 10.09466915130615 }, "id": "APKECPOBEN_656", "locus": "GCA002090215_00620" }, { "type": "cds", "sequence": "contig_1", "start": 351562, "stop": 351822, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACCTTAGTCGGTTTGCCGTTTGAAAAAGGGGAATATCATAAGCAACTGTATGCGCATTCCCGTCAAGAGATTTTGGATCTAGTGGAAGCGGTCAAAAAGCTGTATCTCAGACCAGAAAAGGATGACAAACTGGAGTTTGCTGATTCTTCCAAGGTAAGTGAGTCTAAGAGTATTAAGGTAACCAATGCAGTAACCTTACCAAGTGATGATTTAGATAAAGAGCTGTTTGAATTTAGCGGGGAAGGGGGTGATGAATAA", "aa": "MTLVGLPFEKGEYHKQLYAHSRQEILDLVEAVKKLYLRPEKDDKLEFADSSKVSESKSIKVTNAVTLPSDDLDKELFEFSGEGGDE", "aa_hexdigest": "5d5f7dade4ba0bf05da7074ab771a95f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9733.791899999997, "isoelectric_point": 4.793652915954591 }, "id": "APKECPOBEN_657", "locus": "GCA002090215_00621" }, { "type": "cds", "sequence": "contig_1", "start": 351822, "stop": 352004, "strand": "+", "frame": 3, "gene": "rpsU", "product": "30S ribosomal protein S21", "db_xrefs": [ "COG:COG0828", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:1990904", "RefSeq:WP_010874653.1", "SO:0001217", "UniParc:UPI0000134E7D", "UniRef:UniRef100_P57079", "UniRef:UniRef50_Q8EUK4", "UniRef:UniRef90_P57079" ], "nt": "ATGCCAAAGATTGAAGTTAAAAACGATGATTTAGAGTTAGCTTTAAAAAAGTTTAAACGGGTTTCGCTAGAAATTCGCCGTTTAGCACAGCGTCACGAATACCACCTTCGAAAAGGAATGCGCTTGCGTGAAAAGCGAAAGATTGCGCAGAAGAAACGCCGTAAGTTTCGTAATATGGTCTAG", "aa": "MPKIEVKNDDLELALKKFKRVSLEIRRLAQRHEYHLRKGMRLREKRKIAQKKRRKFRNMV", "aa_hexdigest": "225e633bab1c8eb7c1d04d1005d5a625", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000134E7D", "ncbi_nrp_id": "WP_010874653.1", "uniref100_id": "UniRef100_P57079", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000134E7D", "RefSeq:WP_010874653.1", "UniRef:UniRef100_P57079" ] }, "ips": { "uniref100_id": "UniRef100_P57079", "uniref90_id": "UniRef90_P57079" }, "psc": { "uniref90_id": "UniRef90_P57079", "gene": "rpsU", "product": "30S ribosomal protein S21", "uniref50_id": "UniRef50_Q8EUK4", "cog_id": "COG0828", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_Q8EUK4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q8EUK4" ], "product": "Small ribosomal subunit protein bS21" }, "genes": [ "rpsU" ], "id": "APKECPOBEN_658", "locus": "GCA002090215_00622" }, { "type": "cds", "sequence": "contig_1", "start": 352029, "stop": 352478, "strand": "+", "frame": 3, "gene": null, "product": "putative MG211-like protein", "db_xrefs": [ "RefSeq:WP_010874654.1", "SO:0001217", "UniParc:UPI0000139480", "UniRef:UniRef100_P75481", "UniRef:UniRef50_P47453", "UniRef:UniRef90_P75481" ], "nt": "ATGGATAACGATAAAAAGGATAAGATTATTTTGAGTGGTGAGTTAACTAACCATCGCTTTAATTTTACCAAGGATGGGGAAAACGGCTATAGTGCTTATGAAGTAGATCGTTTTCTAGATCAGCTAGTTCACACTTTAACCCATTACGAAGCGCAGCGTAACCGTGAGGAGGAGATGAAAACGGCTTACGAAAAGCTCTTTCAAGACCGCGACGAAATTTTAAAGCGTTGCTCCAAACTAGAAGCAGAGCTCAATAACTTTTATGAAAACGGTTATTCCAACCGTGTGTTAATTAGTCGGGTTCAAGCCTTGGAAAACAAGATCGAAAGCCTCCCTAGTGGTCAAAATGACCGTCTAGAGCGCATCGAAAAGTTGCTAAAAAGGGTGATTAAGCACTGGACTGACGGGGAGGATTTATCCTATGGTGACTTCGATGATGATTTCTTTTAA", "aa": "MDNDKKDKIILSGELTNHRFNFTKDGENGYSAYEVDRFLDQLVHTLTHYEAQRNREEEMKTAYEKLFQDRDEILKRCSKLEAELNNFYENGYSNRVLISRVQALENKIESLPSGQNDRLERIEKLLKRVIKHWTDGEDLSYGDFDDDFF", "aa_hexdigest": "5efe52370173e871d49c59c311d74bb0", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000139480", "ncbi_nrp_id": "WP_010874654.1", "uniref100_id": "UniRef100_P75481", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139480", "RefSeq:WP_010874654.1", "UniRef:UniRef100_P75481" ] }, "ips": { "uniref100_id": "UniRef100_P75481", "uniref90_id": "UniRef90_P75481" }, "psc": { "uniref90_id": "UniRef90_P75481", "product": "Uncharacterized protein MG211 homolog", "uniref50_id": "UniRef50_P47453" }, "pscc": { "uniref50_id": "UniRef50_P47453", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47453" ], "product": "Uncharacterized protein MG211" }, "genes": [], "id": "APKECPOBEN_659", "locus": "GCA002090215_00623" }, { "type": "cds", "sequence": "contig_1", "start": 352481, "stop": 352831, "strand": "+", "frame": 2, "gene": "acpS", "product": "holo-ACP synthase", "db_xrefs": [ "COG:COG0736", "COG:I", "EC:2.7.8.7", "GO:0000287", "GO:0005737", "GO:0006633", "GO:0008897", "SO:0001217", "UniRef:UniRef50_P75480", "UniRef:UniRef90_P75480" ], "nt": "ATGATTCTAGGCATAGGGATTGATTTAGTCGAAATCAAACGCTTTGAACAATTAGCTAGACAAACTGATAACTGTTTTGCGAAACGCTTGTTGACTTCAACGGAGTACGCGCACTATGCGAAGCTGCGTAAAGATAGTGAAAAGTCGAGTTTTTTAGCAGTCCACTGGTCATTAAAGGAAGCGATTTATAAGGCTGTTAACCACATCAAGCCATTGTTTAGCCAACTGGAAATTACAAAGAAAAACCAACGTTATAACTGCCAGATTGACCCTAAGATTGAACTTTTGCTCTCGGTTAGTTACAGCAGTAATAACATTACTGCCATCTGTTTGGCACAACAAACACCATGA", "aa": "MILGIGIDLVEIKRFEQLARQTDNCFAKRLLTSTEYAHYAKLRKDSEKSSFLAVHWSLKEAIYKAVNHIKPLFSQLEITKKNQRYNCQIDPKIELLLSVSYSSNNITAICLAQQTP", "aa_hexdigest": "6e0ae46a45c7e4b65b3016037358e773", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75480", "query_cov": 1.0, "subject_cov": 0.9747899159663865, "identity": 1.0, "score": 230.0, "evalue": 1.76e-76, "valid": true, "gene": "acpS", "product": "holo-ACP synthase", "ec_ids": [ "2.7.8.7" ], "uniref50_id": "UniRef50_P75480", "cog_id": "COG0736", "cog_category": "I", "go_ids": [ "GO:0005737", "GO:0008897", "GO:0000287", "GO:0006633" ] }, "pscc": { "uniref50_id": "UniRef50_P75480", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75480" ], "product": "Holo-[acyl-carrier-protein] synthase" }, "genes": [ "acpS" ], "id": "APKECPOBEN_660", "locus": "GCA002090215_00624" }, { "type": "cds", "sequence": "contig_1", "start": 352828, "stop": 353205, "strand": "+", "frame": 1, "gene": "plsC", "product": "putative 1-acyl-sn-glycerol-3-phosphate acyltransferase", "db_xrefs": [ "COG:COG0204", "COG:I", "EC:2.3.1.51", "GO:0003841", "GO:0006654", "GO:0016020", "GO:0016024", "SO:0001217", "UniRef:UniRef50_Q49402", "UniRef:UniRef90_P75479" ], "nt": "ATGAAAAAATTAACACAAGCTTTTTTGCGTTTTTGTCTGAGATTCTTACAACTGCTCAGTTTAGTTTTGGTTTTACCCGTTTTTGTGTTAATGCTCATCTCTAGCCTAATTAGTGCTAAAAATTATGAAAGCATCCCGGAAAACTATCCCCCAGAAATCCGCTTTAAGAAGGTGTACCGCTTGGTATCACTTTTCCTCTACATTAAAGGAGTTAAAGTGGTTATTGTTAACCCTGAAAATGTGCCTAAAAAGGCTGTTTTAGTGGTAGCTAATCATAAGTCCAATTTAGATCCCCTCATCCTCATTAAGGCCTTTGGTAAAACTGAAGGGGTACCACCGTTAACCTTTATTGCCAAGATTGAACTGCAAGATACCTGA", "aa": "MKKLTQAFLRFCLRFLQLLSLVLVLPVFVLMLISSLISAKNYESIPENYPPEIRFKKVYRLVSLFLYIKGVKVVIVNPENVPKKAVLVVANHKSNLDPLILIKAFGKTEGVPPLTFIAKIELQDT", "aa_hexdigest": "49f75760ef28420f696218f266615553", "start_type": "ATG", "rbs_motif": null, "genes": [ "plsC" ], "truncated": "3-prime", "pseudogene": { "start": 352828, "stop": 353508, "observations": { "mutation": [ 353203 ], "stop": [ 353203 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75479", "query_cov": 1.0, "subject_cov": 0.4699248120300752, "identity": 1.0, "score": 228.0, "evalue": 1.28e-73, "gene_start": 1, "gene_end": 125, "reference_start": 1, "reference_end": 125, "reference_sequence": "MKKLTQAFLRFCLRFLQLLSLVLVLPVFVLMLISSLISAKNYESIPENYPPEIRFKKVYRLVSLFLYIKGVKVVIVNPENVPKKAVLVVANHKSNLDPLILIKAFGKTEGVPPLTFIAKIELQDTWLFKIMKLIDCVFIDRKNLRQMAASLEQQQQIIRQGTALCVFPEGTRVLSRQIGEFKSGALKVAYNAFVPIVPLTIVGSMGHMESKKRLQKAQVERDRGYKIQVIFNTPINPINFNQIDSQNVANNVWREISQTYAQYCQD" }, "paralog": false, "identity": 0.995575221238938, "score": 434.0, "evalue": 7.65e-157, "description": "Internal stop codon at 353203. Nonsense mutation around 353203.", "psc": { "uniref90_id": "UniRef90_P75479", "gene": "plsC", "product": "Probable 1-acyl-sn-glycerol-3-phosphate acyltransferase", "ec_ids": [ "2.3.1.51" ], "uniref50_id": "UniRef50_Q49402", "cog_id": "COG0204", "cog_category": "I", "go_ids": [ "GO:0016020", "GO:0003841", "GO:0016024", "GO:0006654" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75479", "EC:2.3.1.51", "UniRef:UniRef50_Q49402", "COG:COG0204", "COG:I", "GO:0016020", "GO:0003841", "GO:0016024", "GO:0006654" ] }, "pscc": { "uniref50_id": "UniRef50_Q49402", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49402" ], "product": "Probable 1-acyl-sn-glycerol-3-phosphate acyltransferase" } }, "id": "APKECPOBEN_661", "locus": "GCA002090215_00625" }, { "type": "cds", "sequence": "contig_1", "start": 353218, "stop": 353628, "strand": "+", "frame": 1, "gene": "plsC", "product": "putative 1-acyl-sn-glycerol-3-phosphate acyltransferase", "db_xrefs": [ "COG:COG0204", "COG:I", "EC:2.3.1.51", "GO:0003841", "GO:0006654", "GO:0016020", "GO:0016024", "SO:0001217", "UniRef:UniRef50_Q49402", "UniRef:UniRef90_P75479" ], "nt": "ATGAAGCTGATTGACTGTGTCTTTATTGATCGAAAAAACCTGCGTCAAATGGCAGCATCCTTGGAACAACAGCAGCAAATTATCCGCCAGGGCACTGCTCTGTGTGTTTTTCCTGAGGGTACACGGGTACTCTCTAGGCAAATTGGTGAGTTTAAATCGGGCGCTTTAAAGGTCGCTTACAACGCCTTTGTGCCGATTGTGCCACTAACGATTGTGGGTAGCATGGGACACATGGAGTCCAAAAAGCGTTTACAAAAAGCCCAGGTAGAGCGCGATCGTGGTTATAAAATCCAGGTCATCTTTAACACACCTATTAACCCAATTAACTTTAACCAGATTGATTCGCAAAATGTGGCTAATAATGTCTGGCGTGAAATTAGTCAAACTTATGCGCAATATTGCCAAGACTAG", "aa": "MKLIDCVFIDRKNLRQMAASLEQQQQIIRQGTALCVFPEGTRVLSRQIGEFKSGALKVAYNAFVPIVPLTIVGSMGHMESKKRLQKAQVERDRGYKIQVIFNTPINPINFNQIDSQNVANNVWREISQTYAQYCQD", "aa_hexdigest": "5b0247043a500f3e2c5ff4c822c7bc1a", "start_type": "ATG", "rbs_motif": null, "genes": [ "plsC" ], "truncated": "5-prime", "pseudogene": { "start": 352918, "stop": 353628, "observations": { "mutation": [ 353203 ], "stop": [ 353203 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75479", "query_cov": 1.0, "subject_cov": 0.5112781954887218, "identity": 1.0, "score": 268.0, "evalue": 4.49e-89, "gene_start": 1, "gene_end": 136, "reference_start": 131, "reference_end": 266, "reference_sequence": "MKKLTQAFLRFCLRFLQLLSLVLVLPVFVLMLISSLISAKNYESIPENYPPEIRFKKVYRLVSLFLYIKGVKVVIVNPENVPKKAVLVVANHKSNLDPLILIKAFGKTEGVPPLTFIAKIELQDTWLFKIMKLIDCVFIDRKNLRQMAASLEQQQQIIRQGTALCVFPEGTRVLSRQIGEFKSGALKVAYNAFVPIVPLTIVGSMGHMESKKRLQKAQVERDRGYKIQVIFNTPINPINFNQIDSQNVANNVWREISQTYAQYCQD" }, "paralog": false, "identity": 0.9957627118644068, "score": 461.0, "evalue": 1.28e-167, "description": "Internal stop codon at 353203. Nonsense mutation around 353203.", "psc": { "uniref90_id": "UniRef90_P75479", "gene": "plsC", "product": "Probable 1-acyl-sn-glycerol-3-phosphate acyltransferase", "ec_ids": [ "2.3.1.51" ], "uniref50_id": "UniRef50_Q49402", "cog_id": "COG0204", "cog_category": "I", "go_ids": [ "GO:0016020", "GO:0003841", "GO:0016024", "GO:0006654" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75479", "EC:2.3.1.51", "UniRef:UniRef50_Q49402", "COG:COG0204", "COG:I", "GO:0016020", "GO:0003841", "GO:0016024", "GO:0006654" ] }, "pscc": { "uniref50_id": "UniRef50_Q49402", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49402" ], "product": "Probable 1-acyl-sn-glycerol-3-phosphate acyltransferase" } }, "id": "APKECPOBEN_662", "locus": "GCA002090215_00626" }, { "type": "cds", "sequence": "contig_1", "start": 353752, "stop": 354081, "strand": "+", "frame": 1, "gene": null, "product": "dihydrofolate reductase", "db_xrefs": [ "EC:1.5.1.3", "KEGG:K00287", "SO:0001217", "UniRef:UniRef50_UPI000A8432F8", "UniRef:UniRef90_UPI000A8432F8" ], "nt": "ATGGGTTACAACACCTTTCAGGAGTTAGGTGACAAGCTAACTGATCACAACGTGGTGGTGTTGAGTAAAAAACACTTTGAAGAATTGCAAAACAACACCAACATTAAGGTGTTCAATTCAATTGAAAAGCTCTTGCAACACCACTTTAACCGTGATTTGTATGTGATTGGGGGCAAGCAAATCTTCCACCACTTTATTGAACTGGCTGATCGTTTAATTATTTCGGTTTTACCAGTGGACTTTAAGTGTAACCTTCGCTTAAAGTTGGGCTTGGACAGCTTTGAATTAATGCAAGAACAACAACACAGCCAATTTAAGGTGCAGTACTGA", "aa": "MGYNTFQELGDKLTDHNVVVLSKKHFEELQNNTNIKVFNSIEKLLQHHFNRDLYVIGGKQIFHHFIELADRLIISVLPVDFKCNLRLKLGLDSFELMQEQQHSQFKVQY", "aa_hexdigest": "583e60cf937ca482f3b2525aa60a4782", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 353629, "stop": 354166, "observations": { "insertion": [ 354133 ], "mutation": [ 353692, 354079 ], "stop": [ 353692, 354079 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000A8432F8", "query_cov": 1.0, "subject_cov": 0.6089385474860335, "identity": 1.0, "score": 221.0, "evalue": 2.17e-72, "gene_start": 1, "gene_end": 109, "reference_start": 42, "reference_end": 150, "reference_sequence": "MITLIWCQDKHFGIGRDNTIPWKLTEANQHFYNTTKNQTVVMGYNTFQELGDKLTDHNVVVLSKKHFEELQNNTNIKVFNSIEKLLQHHFNRDLYVIGGKQIFHHFIELADRLIISVLPVDFKCNLRLKLGLDSFELMQEQQHSQFKVQYWHKKHPERLSFNVFLEDYXMGLYQTCWNF" }, "paralog": false, "identity": 0.9832402234636871, "score": 357.0, "evalue": 5.09e-128, "description": "Internal stop codon at 353692, 354079. Frameshift due to insertion around 354133. Nonsense mutation around 353692, 354079.", "psc": { "uniref90_id": "UniRef90_UPI000A8432F8", "product": "dihydrofolate reductase", "ec_ids": [ "1.5.1.3" ], "uniref50_id": "UniRef50_UPI000A8432F8", "kegg_orthology_id": "K00287", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000A8432F8", "EC:1.5.1.3", "UniRef:UniRef50_UPI000A8432F8", "KEGG:K00287" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000A8432F8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000A8432F8" ], "product": "dihydrofolate reductase" } }, "id": "APKECPOBEN_663", "locus": "GCA002090215_00627" }, { "type": "cds", "sequence": "contig_1", "start": 354121, "stop": 354612, "strand": "+", "frame": 1, "gene": "scpA", "product": "segregation/condensation protein A", "db_xrefs": [ "KEGG:K05896", "SO:0001217", "UniRef:UniRef50_UPI000AF5CFDF", "UniRef:UniRef90_UPI000AF5CFDF" ], "nt": "TTGGAAGATTACAATGGGACTTTACCAAACTTGCTGGAACTTTTAATTGATAAGAAGTTCAACTTGCACCAGGTCGATATTGCCAAAATTACGACACAATACCTCCACTTAATTAACACTAACTTAAATAAACAAGCAATTGAACCAATTACCGACTACCTTGTCATTACTAGTCGAATTGTGGAGCAGAAGGCCAATAACTTACTGCAAATTAATGATATTGCCCTAGACTCTGACTTTTTAGACAACAAGCTGCGTGATAAACTAGTAGCACAGTTAGTGGAGTACAAACGTTACCGTGAATCACTCGATGACTTTGAGAAGCTAAGGATCAATCGCTTAGCTTACTTTTCCAAAGATAATGACTTTAACCGCTTTATCCAAACAGTTGACAAGAGTAATACAGAACCCGTGAAAATTGAGGACGAGCTACCCAATTATGTAAGTGTCTTAAAGTTGCATCACGCCATGAATAAGCTAATGCAGCGCTGA", "aa": "MEDYNGTLPNLLELLIDKKFNLHQVDIAKITTQYLHLINTNLNKQAIEPITDYLVITSRIVEQKANNLLQINDIALDSDFLDNKLRDKLVAQLVEYKRYRESLDDFEKLRINRLAYFSKDNDFNRFIQTVDKSNTEPVKIEDELPNYVSVLKLHHAMNKLMQR", "aa_hexdigest": "a1dd2b5742512480cabdfbcb33583015", "start_type": "TTG", "rbs_motif": null, "genes": [ "scpA" ], "truncated": "3-prime", "pseudogene": { "start": 354151, "stop": 354915, "observations": { "mutation": [ 354610 ], "stop": [ 354610 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000AF5CFDF", "query_cov": 0.9386503067484663, "subject_cov": 0.4608433734939759, "identity": 0.993, "score": 299.0, "evalue": 8.16e-100, "gene_start": 11, "gene_end": 163, "reference_start": 1, "reference_end": 153, "reference_sequence": "MLELLIDKKFNLHQVDIAKITTQYLHLINTNLNKQAIEPITDYLVITSRIVEQKANNLLQINDIALDSDFLDNKLRDKLVAQLVEYKRYRESLDDFEKLRINRLAYFSKDNDFNRFIQTVDKSNTEPVKIEDELPNYVSVLKLHHAMNKLMQRWRAQFLANKNISIQELSIEQVQAEILATIKQFGYHSVSLKRVLLKVNHHISLMYFITAFVALLVLINNQIIDIEQTSFDDELYICLLDSSRIEQLQETPEAMVERAVKQRQEAQELARQVAREKAIANAQKREAYLKAKYGKDYLTREQFLKLSPEERAAHVAKMKQLKLVKNDNGRDN" }, "paralog": false, "identity": 0.9921259842519685, "score": 487.0, "evalue": 3.57e-176, "description": "Internal stop codon at 354610. Nonsense mutation around 354610.", "psc": { "uniref90_id": "UniRef90_UPI000AF5CFDF", "gene": "scpA", "product": "segregation/condensation protein A", "uniref50_id": "UniRef50_UPI000AF5CFDF", "kegg_orthology_id": "K05896", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000AF5CFDF", "UniRef:UniRef50_UPI000AF5CFDF", "KEGG:K05896" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000AF5CFDF", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000AF5CFDF" ], "product": "segregation/condensation protein A" } }, "id": "APKECPOBEN_664", "locus": "GCA002090215_00628" }, { "type": "cds", "sequence": "contig_1", "start": 354685, "stop": 355149, "strand": "+", "frame": 1, "gene": "scpA", "product": "segregation/condensation protein A", "db_xrefs": [ "KEGG:K05896", "SO:0001217", "UniRef:UniRef50_UPI000AF5CFDF", "UniRef:UniRef90_UPI000AF5CFDF" ], "nt": "TTGGCTACAATTAAGCAATTTGGCTATCATAGTGTTTCGTTAAAACGGGTACTGTTAAAGGTCAACCACCACATTTCCTTAATGTATTTCATTACCGCTTTTGTCGCTTTATTGGTGTTAATTAACAACCAGATTATCGACATTGAGCAGACTAGCTTTGATGATGAGCTTTACATCTGCTTGTTAGACAGTTCGCGGATTGAACAGCTCCAAGAAACGCCAGAAGCCATGGTGGAACGTGCTGTGAAACAACGCCAGGAAGCACAGGAATTAGCCCGTCAAGTAGCTAGAGAAAAAGCAATTGCCAACGCGCAGAAACGCGAAGCTTACCTAAAGGCAAAATATGGCAAGGATTACTTAACACGTGAACAGTTTTTAAAACTGTCTCCCGAAGAACGTGCTGCCCATGTGGCTAAAATGAAACAATTAAAACTAGTAAAGAACGACAATGGACGCGACAATTAA", "aa": "MATIKQFGYHSVSLKRVLLKVNHHISLMYFITAFVALLVLINNQIIDIEQTSFDDELYICLLDSSRIEQLQETPEAMVERAVKQRQEAQELARQVAREKAIANAQKREAYLKAKYGKDYLTREQFLKLSPEERAAHVAKMKQLKLVKNDNGRDN", "aa_hexdigest": "810ffee5a3e78f552100c53f6bedccb9", "start_type": "TTG", "rbs_motif": "AACAA", "genes": [ "scpA" ], "truncated": "5-prime", "pseudogene": { "start": 354385, "stop": 355149, "observations": { "mutation": [ 354610 ], "stop": [ 354610 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000AF5CFDF", "query_cov": 1.0, "subject_cov": 0.463855421686747, "identity": 0.9940000000000001, "score": 275.0, "evalue": 2.06e-90, "gene_start": 1, "gene_end": 154, "reference_start": 179, "reference_end": 332, "reference_sequence": "MLELLIDKKFNLHQVDIAKITTQYLHLINTNLNKQAIEPITDYLVITSRIVEQKANNLLQINDIALDSDFLDNKLRDKLVAQLVEYKRYRESLDDFEKLRINRLAYFSKDNDFNRFIQTVDKSNTEPVKIEDELPNYVSVLKLHHAMNKLMQRWRAQFLANKNISIQELSIEQVQAEILATIKQFGYHSVSLKRVLLKVNHHISLMYFITAFVALLVLINNQIIDIEQTSFDDELYICLLDSSRIEQLQETPEAMVERAVKQRQEAQELARQVAREKAIANAQKREAYLKAKYGKDYLTREQFLKLSPEERAAHVAKMKQLKLVKNDNGRDN" }, "paralog": false, "identity": 0.9960629921259843, "score": 487.0, "evalue": 2.53e-176, "description": "Internal stop codon at 354610. Nonsense mutation around 354610.", "psc": { "uniref90_id": "UniRef90_UPI000AF5CFDF", "gene": "scpA", "product": "segregation/condensation protein A", "uniref50_id": "UniRef50_UPI000AF5CFDF", "kegg_orthology_id": "K05896", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000AF5CFDF", "UniRef:UniRef50_UPI000AF5CFDF", "KEGG:K05896" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000AF5CFDF", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000AF5CFDF" ], "product": "segregation/condensation protein A" } }, "id": "APKECPOBEN_665", "locus": "GCA002090215_00629" }, { "type": "cds", "sequence": "contig_1", "start": 355133, "stop": 355759, "strand": "+", "frame": 2, "gene": "scpB", "product": "SMC-Scp complex subunit ScpB", "db_xrefs": [ "COG:COG1386", "COG:K", "GO:0005515", "GO:0005737", "GO:0006260", "GO:0007059", "GO:0051301", "GO:0051304", "KEGG:K06024", "RefSeq:WP_010874658.1", "SO:0001217", "UniParc:UPI0000139488", "UniRef:UniRef100_P75477", "UniRef:UniRef50_P75477", "UniRef:UniRef90_P75477" ], "nt": "ATGGACGCGACAATTAAGGTAACTAAACCCGTTTTAAAACAAAAGGATAGCAGCGCTGCTAATTTAGTTGCTGCCATTTATGGGTTGTTGTTTGTAAGTGGCGAAAAGGGCTTAACGTTAGCGGAATTAAACCGCGTATTGCGCAAGGTGGGTTTGGAAAAGATTAAGGCCGCTTTAGTGCAGTTAGAGCGCAAGTTAAGTTTAGATGATGAAAGCGGGATTGAAATTAAAAAGTTTGGTCACAGCTTTCGCTTGGTCACCAAAATGGAGATCAAGGACTTTATCCACCGTTACCTACCCAACAAGATTAAAAATCCGCTCAATTCCAAAACAATGGAAGTGTTAGCAATTATTGCTTACAACCAACCCTGCACCCGTCCGCGCATTAATGAAATTAGGGGTGCAGATTCCTTTCAGATTGTCGATGACTTACTAGAAAAGGAGTTAATTGTTGAATTGGGCCGCAAAGACACACCTGGTCGTCCCTTTATTTATGAAGTGTCGCCTAAGTTCTATGATCTGTTTGGGATTAACAGCTTGGATGAACTGCCTAAAGTCGAGAATTTTGATCTCGACAAATTCCGCCAAGGTAGCTTTTTTGACTCTAACCGTTACGGGGATGATTAA", "aa": "MDATIKVTKPVLKQKDSSAANLVAAIYGLLFVSGEKGLTLAELNRVLRKVGLEKIKAALVQLERKLSLDDESGIEIKKFGHSFRLVTKMEIKDFIHRYLPNKIKNPLNSKTMEVLAIIAYNQPCTRPRINEIRGADSFQIVDDLLEKELIVELGRKDTPGRPFIYEVSPKFYDLFGINSLDELPKVENFDLDKFRQGSFFDSNRYGDD", "aa_hexdigest": "79f84eaf58ca309c2c01271745e51478", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000139488", "ncbi_nrp_id": "WP_010874658.1", "uniref100_id": "UniRef100_P75477", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139488", "RefSeq:WP_010874658.1", "UniRef:UniRef100_P75477" ] }, "ips": { "uniref100_id": "UniRef100_P75477", "uniref90_id": "UniRef90_P75477" }, "psc": { "uniref90_id": "UniRef90_P75477", "gene": "scpB", "product": "SMC-Scp complex subunit ScpB", "uniref50_id": "UniRef50_P75477", "cog_id": "COG1386", "cog_category": "K", "kegg_orthology_id": "K06024", "go_ids": [ "GO:0005515", "GO:0005737", "GO:0006260", "GO:0007059", "GO:0051301", "GO:0051304" ] }, "pscc": { "uniref50_id": "UniRef50_P75477", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75477" ], "product": "Segregation and condensation protein B" }, "genes": [ "scpB" ], "id": "APKECPOBEN_666", "locus": "GCA002090215_00630" }, { "type": "cds", "sequence": "contig_1", "start": 355779, "stop": 356765, "strand": "+", "frame": 3, "gene": "pfkA", "product": "putative ATP-dependent 6-phosphofructokinase", "db_xrefs": [ "COG:COG0205", "COG:G", "EC:2.7.1.11", "GO:0003872", "GO:0005524", "GO:0005829", "GO:0005945", "GO:0006002", "GO:0016208", "GO:0030388", "GO:0042802", "GO:0046872", "GO:0048029", "GO:0061621", "GO:0070095", "RefSeq:WP_010874659.1", "SO:0001217", "UniParc:UPI000012DB5C", "UniRef:UniRef100_P75476", "UniRef:UniRef50_P75476", "UniRef:UniRef90_P75476" ], "nt": "ATGAGTCCAAAAACAACCAAAAAAATTGCCATTTTAACCTCTGGTGGTGATGCTCCAGGGATGAATGCCACACTAGTTTACCTAACGCGTTATGCCACTAGTTCTGAAATTGAAGTTTTCTTTGTGAAAAACGGTTATTATGGGCTGTACCATGATGAGTTAGTACCCGCTCATCAATTAGATTTGTCAAACAGCTTGTTTAGTGCTGGCACCGTGATTGGTAGTAAGCGCTTTGTCGAATTTAAGGAGTTAAAGGTCCGCGAGCAAGCAGCACAGAATCTTAAAAAGCGCCAAATCGATTACCTAGTGGTTATTGGTGGTGATGGTAGTTACATGGGCGCTAAATTGCTAAGCGAGTTGGGCGTTAACTGTTACTGTTTGCCGGGTACGATTGACAACGACATAAATTCGAGTGAGTTTACAATTGGTTTTCTCACGGCTTTGGAGTCGATTAAGGTCAATGTCCAGGCCGTGTACCACACTACGAAATCACATGAACGGGTCGCCATTGTGGAGGTGATGGGTCGCCATTGTGGTGATTTAGCCATTTTTGGTGCCTTAGCCACCAATGCTGACTTTGTGGTGACTCCTAGCAATAAAATGGATTTAAAGCAGCTAGAAAGTGCTGTCAAAAAGATCTTGCAACACCAAAACCACTGTGTGGTGATTGTTAGTGAAAACATCTATGGCTTTGATGGTTATCCCTCACTTACAGCGATCAAACAGCACTTTGATGCTAACAACATGAAGTGTAACCTGGTGTCCTTGGGTCACACGCAACGCGGATTTGCCCCTACCAGCCTCGAGTTAGTCCAAATTAGTTTAATGGCGCAACACACGATTAACTTAATTGGACAAAACAAGGTTAACCAGGTCATTGGTAATAAGGCCAATGTGCCCGTTAACTATGACTTTGATCAAGCCTTTAACATGCCACCGGTTGATCGCAGCGCTTTAATTGCAGTCATTAATAAAAATATTATTTAA", "aa": "MSPKTTKKIAILTSGGDAPGMNATLVYLTRYATSSEIEVFFVKNGYYGLYHDELVPAHQLDLSNSLFSAGTVIGSKRFVEFKELKVREQAAQNLKKRQIDYLVVIGGDGSYMGAKLLSELGVNCYCLPGTIDNDINSSEFTIGFLTALESIKVNVQAVYHTTKSHERVAIVEVMGRHCGDLAIFGALATNADFVVTPSNKMDLKQLESAVKKILQHQNHCVVIVSENIYGFDGYPSLTAIKQHFDANNMKCNLVSLGHTQRGFAPTSLELVQISLMAQHTINLIGQNKVNQVIGNKANVPVNYDFDQAFNMPPVDRSALIAVINKNII", "aa_hexdigest": "626b61bc27bf7ea64beb6d495aa274b8", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000012DB5C", "ncbi_nrp_id": "WP_010874659.1", "uniref100_id": "UniRef100_P75476", "db_xrefs": [ "SO:0001217", "UniParc:UPI000012DB5C", "RefSeq:WP_010874659.1", "UniRef:UniRef100_P75476" ] }, "ips": { "uniref100_id": "UniRef100_P75476", "uniref90_id": "UniRef90_P75476" }, "psc": { "uniref90_id": "UniRef90_P75476", "gene": "pfkA", "product": "Probable ATP-dependent 6-phosphofructokinase", "ec_ids": [ "2.7.1.11" ], "uniref50_id": "UniRef50_P75476", "cog_id": "COG0205", "cog_category": "G", "go_ids": [ "GO:0005945", "GO:0005829", "GO:0003872", "GO:0016208", "GO:0005524", "GO:0070095", "GO:0042802", "GO:0046872", "GO:0048029", "GO:0061621", "GO:0030388", "GO:0006002" ] }, "pscc": { "uniref50_id": "UniRef50_P75476", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75476" ], "product": "Probable ATP-dependent 6-phosphofructokinase" }, "genes": [ "pfkA" ], "id": "APKECPOBEN_667", "locus": "GCA002090215_00631" }, { "type": "cds", "sequence": "contig_1", "start": 356849, "stop": 357634, "strand": "+", "frame": 2, "gene": "pyk", "product": "pyruvate kinase", "db_xrefs": [ "COG:COG0469", "COG:G", "EC:2.7.1.40", "GO:0000287", "GO:0004743", "GO:0005524", "GO:0006096", "GO:0016020", "GO:0016301", "GO:0030955", "GO:0031639", "KEGG:K00873", "SO:0001217", "UniRef:UniRef50_P78031", "UniRef:UniRef90_P78031" ], "nt": "ATGTTAGATGACCCAGCTTACGCTGCGATGAAAGCAGAAGCTTACGCCAACATTGAAAATATTATTAAAAACGGCGTAACGGTAATCCGCTTAAACTTCTCCCATGGTAACCATGAAGAGCAGGCTGTAAGGATTAAGATTGTTCGTGATGTGGCTAAAAAGCTCAACCTCCCGGTGTCAATTATGTTAGACACCAACGGTCCGGAAATCCGCGTCTTTGAAACGGCCCCTGAAGGGTTAAAGATCCTCAAGGACAGTGAGGTGGTTATTAACACCACCACTAAGGAAGTGGCTAAAAATAACCAGTTTAGTGTGAGTGATGCTAGTGGCACTTACAACATGGTAAACGATGTTAAGGTGGGACAAAAAATCCTCGTTGATGATGGTAAGTTAAGCTTAGTCGTAAAGCGGATTGATACGAAGAACAACCAGGTTATTTGTGTTGCCCAAAATGACCACACTATCTTTACCAAAAAGCGCTTAAACCTCCCCAATGCCGACTACTCAATTCCCTTTTTAAGTGCTAAAGACCTACGCGACATTGACTTTGGTTTAACCCACCAAATTGACTACATTGCCGCTTCCTTTGTCAATACAACGGAAAACATCAAGCAACTGCGTGACTACCTCGCTTCTAAGAATGCCAAGCATGTCAAGCTTATTGCCAAAATTGAGTCCAACCACGCTTTAAACAACATTGATGGGATTATTAAAGCGAGTGATGGCATTATGGTAGCACGGGGTGATTTGGGCTTGGAGATCCCATATTACAAGGTGCCTTACTGA", "aa": "MLDDPAYAAMKAEAYANIENIIKNGVTVIRLNFSHGNHEEQAVRIKIVRDVAKKLNLPVSIMLDTNGPEIRVFETAPEGLKILKDSEVVINTTTKEVAKNNQFSVSDASGTYNMVNDVKVGQKILVDDGKLSLVVKRIDTKNNQVICVAQNDHTIFTKKRLNLPNADYSIPFLSAKDLRDIDFGLTHQIDYIAASFVNTTENIKQLRDYLASKNAKHVKLIAKIESNHALNNIDGIIKASDGIMVARGDLGLEIPYYKVPY", "aa_hexdigest": "6cb764dfa725510c574b570bf5492780", "start_type": "ATG", "rbs_motif": null, "genes": [ "pyk" ], "truncated": "both", "pseudogene": { "start": 356771, "stop": 357937, "observations": { "mutation": [ 356837, 357632 ], "stop": [ 356837, 357632 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P78031", "query_cov": 1.0, "subject_cov": 0.5137795275590551, "identity": 1.0, "score": 509.0, "evalue": 1.28e-178, "gene_start": 1, "gene_end": 261, "reference_start": 27, "reference_end": 287, "reference_sequence": "MIHHLKRTKIIATCGPALTKKLWTLAMLDDPAYAAMKAEAYANIENIIKNGVTVIRLNFSHGNHEEQAVRIKIVRDVAKKLNLPVSIMLDTNGPEIRVFETAPEGLKILKDSEVVINTTTKEVAKNNQFSVSDASGTYNMVNDVKVGQKILVDDGKLSLVVKRIDTKNNQVICVAQNDHTIFTKKRLNLPNADYSIPFLSAKDLRDIDFGLTHQIDYIAASFVNTTENIKQLRDYLASKNAKHVKLIAKIESNHALNNIDGIIKASDGIMVARGDLGLEIPYYKVPYWQRYMIKACRFFNKRVITATQMLDSLEKNIQPTRAEVTDVYFAVDRGNDATMLSGETANGAFPLNAVYVMKMIDKQSETFFDYQYNLNYYMANSKARHSEFWKQVVLPLAQKTAPKRKLINSDFKYDFVVHATNNLNEIYALSNARLAAAVIILTNDPQVYTGHGVDYGIFPYLIDQKPQSLSKAEFKSLANVAIKHYQQHGEISQLKQCLGVFHNKIISL" }, "paralog": false, "identity": 0.9948453608247423, "score": 758.0, "evalue": 7.52e-279, "description": "Internal stop codon at 356837, 357632. Nonsense mutation around 356837, 357632.", "psc": { "uniref90_id": "UniRef90_P78031", "gene": "pyk", "product": "pyruvate kinase", "ec_ids": [ "2.7.1.40" ], "uniref50_id": "UniRef50_P78031", "cog_id": "COG0469", "cog_category": "G", "kegg_orthology_id": "K00873", "go_ids": [ "GO:0000287", "GO:0004743", "GO:0005524", "GO:0006096", "GO:0016020", "GO:0016301", "GO:0030955", "GO:0031639" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P78031", "EC:2.7.1.40", "UniRef:UniRef50_P78031", "COG:COG0469", "COG:G", "KEGG:K00873", "GO:0000287", "GO:0004743", "GO:0005524", "GO:0006096", "GO:0016020", "GO:0016301", "GO:0030955", "GO:0031639" ] }, "pscc": { "uniref50_id": "UniRef50_P78031", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P78031" ], "product": "Pyruvate kinase" } }, "id": "APKECPOBEN_668", "locus": "GCA002090215_00632" }, { "type": "cds", "sequence": "contig_1", "start": 357644, "stop": 357937, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00224.27" ], "nt": "ATGATAAAAGCGTGTCGTTTCTTTAACAAGCGTGTTATTACCGCAACTCAAATGTTGGACTCTTTGGAAAAAAACATCCAACCAACTCGCGCTGAAGTTACTGATGTGTACTTTGCCGTGGACCGCGGCAATGATGCCACCATGTTAAGTGGAGAAACCGCAAATGGGGCGTTTCCCCTTAATGCTGTGTATGTAATGAAGATGATTGATAAACAGTCCGAAACGTTCTTTGATTACCAGTACAACTTGAACTATTACATGGCCAACTCCAAGGCCCGTCACAGTGAGTTTTGA", "aa": "MIKACRFFNKRVITATQMLDSLEKNIQPTRAEVTDVYFAVDRGNDATMLSGETANGAFPLNAVYVMKMIDKQSETFFDYQYNLNYYMANSKARHSEF", "aa_hexdigest": "7c59f3e088c1d3bc519e2f601048efa3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00224.27", "name": "PK", "length": 63, "aa_cov": 0.6494845360824743, "hmm_cov": 1.0, "evalue": 1.5884854540259187e-24, "score": 82.03192901611328, "start": 1, "stop": 63 } ], "seq_stats": { "molecular_weight": 11198.605099999997, "isoelectric_point": 6.551739692687988 }, "id": "APKECPOBEN_669", "locus": "GCA002090215_00633" }, { "type": "cds", "sequence": "contig_1", "start": 357944, "stop": 358297, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGTGTTACCGTTAGCACAAAAAACAGCACCAAAACGCAAACTGATTAACAGTGACTTTAAATACGACTTTGTGGTGCACGCCACCAACAACCTTAATGAAATCTATGCCTTGTCCAACGCTCGCTTAGCGGCTGCGGTAATTATCTTGACCAATGACCCCCAAGTATACACCGGGCACGGCGTTGATTATGGCATCTTCCCTTACTTAATTGACCAAAAGCCGCAAAGCTTAAGTAAAGCTGAGTTTAAGAGCTTAGCTAATGTTGCCATTAAACACTACCAGCAGCATGGGGAAATTAGTCAATTAAAGCAGTGTTTGGGAGTATTTCACAATAAGATCATTAGCTTATAA", "aa": "MVLPLAQKTAPKRKLINSDFKYDFVVHATNNLNEIYALSNARLAAAVIILTNDPQVYTGHGVDYGIFPYLIDQKPQSLSKAEFKSLANVAIKHYQQHGEISQLKQCLGVFHNKIISL", "aa_hexdigest": "e13dcbdd189b0752fd87ad3cd15a034b", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13110.00559999999, "isoelectric_point": 9.240269660949707 }, "id": "APKECPOBEN_670", "locus": "GCA002090215_00634" }, { "type": "tRNA", "sequence": "contig_1", "start": 358337, "stop": 358413, "strand": "+", "gene": "trnC", "product": "tRNA-Cys(gca)", "amino_acid": "Cys", "anti_codon": "gca", "score": 60.0, "nt": "GGTGCCTTAGCCAAGTGGATTCAAGGCCTGGAGCTGCAACCTCCATATCGTCAGTTCGAATCTGACAGGCACCTCCA", "db_xrefs": [ "SO:0000258" ], "anti_codon_pos": [ 358372, 358374 ], "id": "APKECPOBEN_1", "locus": "GCA002090215_00635" }, { "type": "tRNA", "sequence": "contig_1", "start": 358465, "stop": 358541, "strand": "+", "gene": "trnP", "product": "tRNA-Pro(tgg)", "amino_acid": "Pro", "anti_codon": "tgg", "score": 83.1, "nt": "CGGGAAGTAGCTTAGTTTGGTAGAGCACTTGGTTTGGGACCAAGGGGTCGCAGGTTCAAATCCTGTCTTCCCGACCA", "db_xrefs": [ "SO:0000268" ], "anti_codon_pos": [ 358482, 358484 ], "id": "APKECPOBEN_2", "locus": "GCA002090215_00636" }, { "type": "tRNA", "sequence": "contig_1", "start": 358546, "stop": 358622, "strand": "+", "gene": "trnM", "product": "tRNA-Met(cat)", "amino_acid": "Met", "anti_codon": "cat", "score": 82.2, "nt": "GGCTGGATACCTCAGTTGGTTAGAGGACCCGGTTCATACCCGGGTTGTCGTGAGTTCGAATCTCACTCCAGCCACCA", "db_xrefs": [ "SO:0000266" ], "anti_codon_pos": [ 358580, 358582 ], "id": "APKECPOBEN_3", "locus": "GCA002090215_00637" }, { "type": "tRNA", "sequence": "contig_1", "start": 358633, "stop": 358708, "strand": "+", "gene": "trnI", "product": "tRNA-Ile2(cat)", "amino_acid": "Ile2", "anti_codon": "cat", "score": 74.7, "nt": "GGATCTATAGCTCAATGGTTAGAGCCCCCGACTCATAATCGGTCTGTTACAGGTTCGAGTCCTGTTAGATCCACCA", "db_xrefs": [ "SO:0000263" ], "id": "APKECPOBEN_4", "locus": "GCA002090215_00638" }, { "type": "tRNA", "sequence": "contig_1", "start": 358749, "stop": 358839, "strand": "+", "gene": "trnS", "product": "tRNA-Ser(tga)", "amino_acid": "Ser", "anti_codon": "tga", "score": 78.9, "nt": "GGAGACTTACCCAAGCGGCTGAAGGGTTCGGTCTTGAAAACCGAGAGGTGCTGAAAAGCACGCGAGGGTTCGAATCCCTCAGTCTCCGCCA", "db_xrefs": [ "SO:0000269" ], "anti_codon_pos": [ 358768, 358770 ], "id": "APKECPOBEN_5", "locus": "GCA002090215_00639" }, { "type": "tRNA", "sequence": "contig_1", "start": 358852, "stop": 358928, "strand": "+", "gene": "trnfM", "product": "tRNA-fMet(cat)", "amino_acid": "fMet", "anti_codon": "cat", "score": 77.7, "nt": "CGCGGGATAGAGCAGTCGGTTAGCTCGTCAGGCTCATAATCTGAAGGTCGGGGGTTCGAATCCCTCTCCCGCAACCA", "db_xrefs": [ "SO:0000266" ], "id": "APKECPOBEN_6", "locus": "GCA002090215_00640" }, { "type": "tRNA", "sequence": "contig_1", "start": 358930, "stop": 359003, "strand": "+", "gene": "trnD", "product": "tRNA-Asp(gtc)", "amino_acid": "Asp", "anti_codon": "gtc", "score": 55.6, "nt": "GGTTCCATGGTGTAGTGATAACATATCTCCCTGTCACGGAGGGGTTGCGGGTTTGATTCCCGTTGGAACCGCCA", "db_xrefs": [ "SO:0000256" ], "anti_codon_pos": [ 358962, 358964 ], "id": "APKECPOBEN_7", "locus": "GCA002090215_00641" }, { "type": "tRNA", "sequence": "contig_1", "start": 359005, "stop": 359080, "strand": "+", "gene": "trnF", "product": "tRNA-Phe(gaa)", "amino_acid": "Phe", "anti_codon": "gaa", "score": 77.8, "nt": "GGTCTTGTAGCTCAGTCGGTAGAGCAACGGTCTGAAGAACCGTGTGTCGGCAGTTCGATTCTGCCCGAGACCACCA", "db_xrefs": [ "SO:0000267" ], "anti_codon_pos": [ 359038, 359040 ], "id": "APKECPOBEN_8", "locus": "GCA002090215_00642" }, { "type": "cds", "sequence": "contig_1", "start": 359277, "stop": 359552, "strand": "+", "frame": 3, "gene": null, "product": "arginine deiminase family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00132F68E0", "UniRef:UniRef90_UPI00132F68E0" ], "nt": "ATGAAGTACAACATCAACGTTCATTCCGAAATTGGTCAACTGCAAACCGTTTTAGTCCACACCCCTGGTAATGAAATTAGACGGATTTCACCAAGACGGTTGGATGATTTACTGTTTTCAGCAGTAATAGAACCCGATACTGCCATCCAGGAACACCAAACCTTTTGTCAGTTACTGCAAGAACAAAACATTGAAGTGGTGCAACTGACTGATTTAACAGCCACCACCTTTGACAAGGCAAATGCTACAGCGCAAAACCAGTTTATTGAAACTTGA", "aa": "MKYNINVHSEIGQLQTVLVHTPGNEIRRISPRRLDDLLFSAVIEPDTAIQEHQTFCQLLQEQNIEVVQLTDLTATTFDKANATAQNQFIET", "aa_hexdigest": "e88084dfe637df752a322788b98e60e0", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 359277, "stop": 359831, "observations": { "mutation": [ 359550 ], "stop": [ 359550 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00132F68E0", "query_cov": 1.0, "subject_cov": 0.4945652173913043, "identity": 1.0, "score": 179.0, "evalue": 9.54e-56, "gene_start": 1, "gene_end": 91, "reference_start": 1, "reference_end": 91, "reference_sequence": "MKYNINVHSEIGQLQTVLVHTPGNEIRRISPRRLDDLLFSAVIEPDTAIQEHQTFCQLLQEQNIEVVQLTDLTATTFDKANATAQNQFIETWLDQAEPKLTPEHRKVAKQYLLEQKAKSTLSMVRSMMGGIDKRKVAAANTINGDFLVDPMPNLYFTRDPFASIGHGISINRMKYLTRRRQTLF" }, "paralog": false, "identity": 0.9891304347826086, "score": 360.0, "evalue": 1.31e-129, "description": "Internal stop codon at 359550. Nonsense mutation around 359550.", "psc": { "uniref90_id": "UniRef90_UPI00132F68E0", "product": "arginine deiminase family protein", "uniref50_id": "UniRef50_UPI00132F68E0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00132F68E0", "UniRef:UniRef50_UPI00132F68E0" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00132F68E0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00132F68E0" ], "product": "arginine deiminase family protein" } }, "id": "APKECPOBEN_671", "locus": "GCA002090215_00643" }, { "type": "cds", "sequence": "contig_1", "start": 359643, "stop": 359948, "strand": "+", "frame": 3, "gene": null, "product": "Arginine deiminase domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAN5BCR3", "UniRef:UniRef90_A0AAN5BCR3" ], "nt": "ATGGTACGCTCGATGATGGGTGGGATTGATAAGCGTAAGGTAGCTGCTGCTAACACCATTAACGGTGATTTTTTAGTAGATCCGATGCCTAACCTTTACTTTACCCGCGATCCGTTTGCCTCGATTGGCCATGGTATTAGTATTAACCGGATGAAGTATTTAACGCGGCGGCGTGAAACCTTATTTGCTAGTTTTATTTTGCCAACCACCCCGATTATTGCCGCACGCAAATTTACTTTAAACCAATTGACATGGGCACAATTGAGGGTGGTGATATTTTTGTATATGACCAACAAACAGTGGTGA", "aa": "MVRSMMGGIDKRKVAAANTINGDFLVDPMPNLYFTRDPFASIGHGISINRMKYLTRRRETLFASFILPTTPIIAARKFTLNQLTWAQLRVVIFLYMTNKQW", "aa_hexdigest": "ef30367e02109f27bddba0f6fdb22e9b", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 359343, "stop": 359993, "observations": { "mutation": [ 359550, 359946, 359955 ], "stop": [ 359550, 359946, 359955 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAN5BCR3", "query_cov": 1.0, "subject_cov": 0.42436974789915966, "identity": 1.0, "score": 206.0, "evalue": 1.27e-65, "gene_start": 1, "gene_end": 101, "reference_start": 123, "reference_end": 223, "reference_sequence": "MKYNINVHSEIGQLQTVLVHTPGNEIRRISPRRLDDLLFSAVIEPDTAIQEHQTFCQLLQEQNIEVVQLTDLTATTFDKANATAQNQFIETWLDQAEPKLTPEHRKVAKQYLLEQKAKSTLSMVRSMMGGIDKRKVAAANTINGDFLVDPMPNLYFTRDPFASIGHGISINRMKYLTRRRETLFASFILPTTPIIAARKFTLNQLTWAQLRVVIFLYMTNKQWWWGWVNGQPKLPLMF" }, "paralog": false, "identity": 0.9861111111111112, "score": 421.0, "evalue": 1.17e-152, "description": "Internal stop codon at 359550, 359946, 359955. Nonsense mutation around 359550, 359946, 359955.", "psc": { "uniref90_id": "UniRef90_A0AAN5BCR3", "product": "Arginine deiminase domain-containing protein", "uniref50_id": "UniRef50_A0AAN5BCR3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAN5BCR3", "UniRef:UniRef50_A0AAN5BCR3" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAN5BCR3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAN5BCR3" ], "product": "Arginine deiminase domain-containing protein" } }, "id": "APKECPOBEN_672", "locus": "GCA002090215_00644" }, { "type": "cds", "sequence": "contig_1", "start": 359894, "stop": 360187, "strand": "+", "frame": 2, "gene": null, "product": "Arginine deiminase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W742", "UniRef:UniRef90_A0AB38W742" ], "nt": "ATGGGCACAATTGAGGGTGGTGATATTTTTGTATATGACCAACAAACAGTGGTGATGGGGTTGAGTGAACGGACAACCGAAGCTGCCATTAATGTTTTAGCGAAAAAGATTCAACAAGACAGTAGCACTTCCTTTAAGCGGATCTTTGTCATTAACGTTCCACAACTACCGAACTTAATGCATTTAGACACGTGGCTGACAATGTTAGACCGCAACAAGTTCTTGTACTCCCCGAACATGTTGGCGGTTTTAAAGGCGTGGCGGATTGACTTTACTGATCCGGCTTTAAAGTGA", "aa": "MGTIEGGDIFVYDQQTVVMGLSERTTEAAINVLAKKIQQDSSTSFKRIFVINVPQLPNLMHLDTWLTMLDRNKFLYSPNMLAVLKAWRIDFTDPALK", "aa_hexdigest": "3822e135b80afeff289e2e8b6fceabe8", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 359894, "stop": 360490, "observations": { "mutation": [ 360185 ], "stop": [ 360185 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB38W742", "query_cov": 1.0, "subject_cov": 0.4898989898989899, "identity": 0.9790000000000001, "score": 191.0, "evalue": 1.82e-60, "gene_start": 1, "gene_end": 97, "reference_start": 1, "reference_end": 97, "reference_sequence": "MGTIEGGDIFVYDQQTVVVGLSERTTEAAINVLAKKIQQDSSTSFKRIFVINVPQLPNLMHLDTWLTMLDRNKFLYSPNMLAVLKAWRIDLTDPALKWNEIAGDLSTILHTIIGQKPMLIPIAGADANQTEIDIETHFDGTNYLTIAPSVVVGYARNKLTHQALEAAGVKVIAFKGNQLSLGMGSARCMSMPLVRKPL" }, "paralog": false, "identity": 0.9797979797979798, "score": 383.0, "evalue": 1.99e-138, "description": "Internal stop codon at 360185. Nonsense mutation around 360185.", "psc": { "uniref90_id": "UniRef90_A0AB38W742", "product": "Arginine deiminase", "uniref50_id": "UniRef50_A0AB38W742", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB38W742", "UniRef:UniRef50_A0AB38W742" ] }, "pscc": { "uniref50_id": "UniRef50_A0AB38W742", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W742" ], "product": "Arginine deiminase" } }, "id": "APKECPOBEN_673", "locus": "GCA002090215_00645" }, { "type": "cds", "sequence": "contig_1", "start": 360245, "stop": 360490, "strand": "+", "frame": 2, "gene": null, "product": "Arginine deiminase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W742", "UniRef:UniRef90_A0AB38W742" ], "nt": "ATGCTCATTCCCATTGCCGGAGCGGATGCTAACCAAACCGAAATTGACATTGAAACCCACTTTGATGGCACCAACTACCTCACCATCGCTCCATCGGTAGTAGTGGGGTATGCGCGCAATAAATTAACACACCAGACCTTGGAAGCGGCTGGGGTTAAAGTAATTGCCTTTAAGGGGAACCAGCTGTCTTTGGGAATGGGTTCTGCACGTTGTATGTCGATGCCATTAGTACGCAAACCACTTTAA", "aa": "MLIPIAGADANQTEIDIETHFDGTNYLTIAPSVVVGYARNKLTHQTLEAAGVKVIAFKGNQLSLGMGSARCMSMPLVRKPL", "aa_hexdigest": "f6012470d71bc0c04d95dcdec9f58ffd", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 359945, "stop": 360490, "observations": { "mutation": [ 360185 ], "stop": [ 360185 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB38W742", "query_cov": 1.0, "subject_cov": 0.4090909090909091, "identity": 0.988, "score": 160.0, "evalue": 1.91e-48, "gene_start": 1, "gene_end": 81, "reference_start": 118, "reference_end": 198, "reference_sequence": "MGTIEGGDIFVYDQQTVVVGLSERTTEAAINVLAKKIQQDSSTSFKRIFVINVPQLPNLMHLDTWLTMLDRNKFLYSPNMLAVLKAWRIDLTDPALKWNEIAGDLSTILHTIIGQKPMLIPIAGADANQTEIDIETHFDGTNYLTIAPSVVVGYARNKLTHQALEAAGVKVIAFKGNQLSLGMGSARCMSMPLVRKPL" }, "paralog": false, "identity": 0.9779005524861878, "score": 349.0, "evalue": 3.82e-125, "description": "Internal stop codon at 360185. Nonsense mutation around 360185.", "psc": { "uniref90_id": "UniRef90_A0AB38W742", "product": "Arginine deiminase", "uniref50_id": "UniRef50_A0AB38W742", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB38W742", "UniRef:UniRef50_A0AB38W742" ] }, "pscc": { "uniref50_id": "UniRef50_A0AB38W742", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W742" ], "product": "Arginine deiminase" } }, "id": "APKECPOBEN_674", "locus": "GCA002090215_00646" }, { "type": "cds", "sequence": "contig_1", "start": 360630, "stop": 360902, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02729.26" ], "nt": "TTGCACATCCAGAACCGTCCCTTAGCGGGGAAGAACATTGTTTTACTGTTCCAAAAAGATTCTACCCGTACCCGTTGTGCCTTTGAAGTGGCTGCCTTTGACTTGGGTATGGGTTGCACTTACATCGGCCCTTCTGGTTCCAATTTTGGTAAGAAGGAATCGATTGAAGACACTGCTAAAGTGTTGGGTTCAATGTATGACGGAATTGAATTCCGTGGCTTTAAGCAAAGCGATGTTGATGCTTTGGTCAAGTACTCTGGTGTTCCTGTTTGA", "aa": "MHIQNRPLAGKNIVLLFQKDSTRTRCAFEVAAFDLGMGCTYIGPSGSNFGKKESIEDTAKVLGSMYDGIEFRGFKQSDVDALVKYSGVPV", "aa_hexdigest": "1608b1a2f24569b297d54d1b9508a3af", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02729.26", "name": "OTCace_N", "length": 92, "aa_cov": 0.9555555555555556, "hmm_cov": 1.0, "evalue": 4.535546992210771e-26, "score": 87.17288208007812, "start": 5, "stop": 90 } ], "seq_stats": { "molecular_weight": 9824.148799999997, "isoelectric_point": 7.761841773986817 }, "id": "APKECPOBEN_675", "locus": "GCA002090215_00647" }, { "type": "cds", "sequence": "contig_1", "start": 360909, "stop": 361241, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00185.30" ], "nt": "TTGACTGATGCGGAACACCCTACCCAAATGCTAGCGGATTACATGACGATTAAGGAGTTGAAGGGTGATTTGAAGGGGCGCAAAATTGTCTTTGCTGGTGATATTAAAAATAATGTGGCACGCTCGTTGATGATTGGGGCAGCTTTTGTTGGTATGGACATAGTGCTAGTAGGTCCTAAAGCACAGCATGAATTAGTTCAAAATGGTGCTGGGTATAAAGAGGTCTTTGAGCAGTGTCAAGCATTGTTTCAACTCAATGGTGGTAGTGTTAGCTTTAGCACTGATAAGCTGCAAGCGGCTAAGAATGCTGATGTAATTTACACCGATGTGTGA", "aa": "MTDAEHPTQMLADYMTIKELKGDLKGRKIVFAGDIKNNVARSLMIGAAFVGMDIVLVGPKAQHELVQNGAGYKEVFEQCQALFQLNGGSVSFSTDKLQAAKNADVIYTDV", "aa_hexdigest": "334c7426cbdec7e6abf218bd0e67c9d4", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00185.30", "name": "OTCace", "length": 86, "aa_cov": 0.7818181818181819, "hmm_cov": 0.9186046511627907, "evalue": 2.5090884006120317e-19, "score": 65.3192138671875, "start": 25, "stop": 110 } ], "seq_stats": { "molecular_weight": 11936.594999999994, "isoelectric_point": 5.569902992248534 }, "id": "APKECPOBEN_676", "locus": "GCA002090215_00648" }, { "type": "cds", "sequence": "contig_1", "start": 361248, "stop": 361559, "strand": "+", "frame": 3, "gene": null, "product": "Ornithine carbamoyltransferase chain F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A4U9HLN2" ], "nt": "TTGGGTGAAGACTTTAGCTTATTTGAAGAACGGATTCAGGAGCTGGGTCAATTTCAAGTTGACATGGCTATGATCAAAGCAGCTAAGAGTGATGTGATCTTCTTGCACTGTCTCCCCGCTTTCCATGATGATCACACTAGCTTTTCACTGGAAATTAAACAAAAGTTAGGTAAAAAATATCCGGTGGTGAAAACGGGTGCGATGGAAGTGACTGATACGGTGTTTCAGTCCAAGCATAACATGGCCTTTATCCAAGCAGAAAACCGCTTGCACACGATTAAGGCCGTTATTTTAGCTACTTTAGGGTATTAA", "aa": "MGEDFSLFEERIQELGQFQVDMAMIKAAKSDVIFLHCLPAFHDDHTSFSLEIKQKLGKKYPVVKTGAMEVTDTVFQSKHNMAFIQAENRLHTIKAVILATLGY", "aa_hexdigest": "f2e0a1078b4b5f84a9b7db0a05cf2ea8", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A4U9HLN2", "query_cov": 0.8349514563106796, "subject_cov": 0.9885057471264368, "identity": 0.5, "score": 79.0, "evalue": 2.91e-17, "valid": false, "product": "Ornithine carbamoyltransferase chain F", "uniref50_id": "UniRef50_A0A4U9HLN2" }, "pscc": { "uniref50_id": "UniRef50_A0A4U9HLN2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A4U9HLN2" ], "product": "Ornithine carbamoyltransferase chain F" }, "genes": [], "id": "APKECPOBEN_677", "locus": "GCA002090215_00649" }, { "type": "cds", "sequence": "contig_1", "start": 361562, "stop": 362491, "strand": "+", "frame": 2, "gene": "arcC", "product": "Carbamate kinase-like protein", "db_xrefs": [ "COG:COG0549", "COG:E", "GO:0005829", "GO:0008804", "GO:0019546", "RefSeq:WP_010874663.1", "SO:0001217", "UniParc:UPI0000125DAF", "UniRef:UniRef100_P78030", "UniRef:UniRef50_P78030", "UniRef:UniRef90_P78030" ], "nt": "ATGCAAAAAATTGTCATAGCATTGGGTGGTAATGCATTAGGTAACAATCCCACCGAACAAAAGGACTTGGTACAACTACCAGCACGACAGGCGGTTGCTTTATTAAAGCAGGGTTATCAGATATTATTGGGTCATGGTAACGGTCCACAGGTTGGCATGATCTACAACGCCTTTAGTGCGGCCAAACAAGTCAACCCTAATACACCCACTGTACCATTTGCTGAAAGTGGGGCAATGTCTCAGGGTTACATTGGACTGCATTTGTTAACAGCATTGTACAATGAGCTGCTACACCAAAAACTACCCCATCAAGCCGTGTACTTTTTAACACAAACCTTAGTAGAAGCGAGCGATCCTGCTTTTCAAAACCCAAATAAACCAGTTGGTCCGTTTTACAACACAGAGGAAACAGCACGCTCAGCTAATCCAAACAGCACCGTGGTGGAAGATGCAGGGCGGGGTTGGCGTAAAGTAGTAGCGTCCCCCAAACCAGTTGATGTTTTGGGAATTGATGCCATTAAAAGTAGCTTTAACCAAGGCAACTTGGTGATTGTTGGTGGCGGTGGCGGTGTACCCACAATTAAGACCAAAAGTGGTTATGCAACGGTTGATGGGGTGATTGATAAGGATCTAGCATTGAGTGAAATTGCCATTAAGGTAGAGGCTGACCTGTTTGTTATTCTCACTGCCGTGGACTTTGTCTACATTAACTATGGTCAACCAAACGAGCAAAAGCTAACCTGCATTAACACCAAGGAAGCTAAGACCTTAATGGCCGCAAACCAGTTTGCTAAAGGTAGTATGCTCCCTAAGGTAGAAGCGTGTTTAAACTTTGTCCAGTCGGGTACCAATAAGACGGCCATTATTGCCCAATTAGACCAATTAGCTGCGGCCATTGCGGGCAAGATTGGTACTAAAATTGTCAAGTAG", "aa": "MQKIVIALGGNALGNNPTEQKDLVQLPARQAVALLKQGYQILLGHGNGPQVGMIYNAFSAAKQVNPNTPTVPFAESGAMSQGYIGLHLLTALYNELLHQKLPHQAVYFLTQTLVEASDPAFQNPNKPVGPFYNTEETARSANPNSTVVEDAGRGWRKVVASPKPVDVLGIDAIKSSFNQGNLVIVGGGGGVPTIKTKSGYATVDGVIDKDLALSEIAIKVEADLFVILTAVDFVYINYGQPNEQKLTCINTKEAKTLMAANQFAKGSMLPKVEACLNFVQSGTNKTAIIAQLDQLAAAIAGKIGTKIVK", "aa_hexdigest": "993d22e63285c239bdd26b61078511c6", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000125DAF", "ncbi_nrp_id": "WP_010874663.1", "uniref100_id": "UniRef100_P78030", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000125DAF", "RefSeq:WP_010874663.1", "UniRef:UniRef100_P78030" ] }, "ips": { "uniref100_id": "UniRef100_P78030", "uniref90_id": "UniRef90_P78030" }, "psc": { "uniref90_id": "UniRef90_P78030", "gene": "ArcC", "product": "Carbamate kinase-like protein", "uniref50_id": "UniRef50_P78030", "cog_id": "COG0549", "cog_category": "E", "go_ids": [ "GO:0005829", "GO:0008804", "GO:0019546" ] }, "pscc": { "uniref50_id": "UniRef50_P78030", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P78030" ], "product": "Carbamate kinase-like protein" }, "genes": [ "arcC" ], "id": "APKECPOBEN_678", "locus": "GCA002090215_00650" }, { "type": "cds", "sequence": "contig_1", "start": 362494, "stop": 363297, "strand": "+", "frame": 1, "gene": null, "product": "amino acid permease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00244EE166" ], "nt": "ATGAAACAACAAAAACCCAAAATTAGCTTTATAGCAGCGATGCTAATTGTTATCGGCTCGTCCATAGGTGCTGGTATCTTTTTTAAATCCAGCACTGTTTTGGAAAATTCCCAGGCCTCTTTAGTGCTAGCGATTTTTAACTGGTTGGTAGCTTCAGTGGCAGTGATTGCTATGGCCTTGGCATTAATTGAAATTGCCAGTGTTCGCAACGATAATCTCTCGATTATTAGCTGGGTCAAAGTGTTTAACCGGCGCTGGTTGTACCATGGTTGTAAGAATTTTATGACCTATTTGTACCTACCGTTGACCTTCTTCTTTATGCCGCTGTACTTTATCTGTTCCATTCAAGATGGGTTTCGCGGTTTGTTGGGACTGGAAACCGGAGCGCATTTCAATACTAGTGTGGACTGGTTAATCTGGTTAGCTTTAGCACTTATTATTACCACTTACTTCTTGACAATTCCACCACTGTACGCTAAGGTAGGGAACATCCAAAACATGGTGGTAAGCGCCGTGAAGTTTATTCCTTTAGTGTTTGTGCCTATCATCGGTTTTATTGTAGCGGGTACAGGTAACGGTGAATTAAAAAACGTTAAGGCATTAGTGCAACCCCCACAAATTAATGGTGCGACTGCTTCCTTTACCCAATTAGTACAAGCGGGGTATGGAATTACTCGTTTTACGGGAATTGGTGCTGGGATGGGTTCCTTTATTTCGATTGCCGCCATTTTCTTTGCGTACGATGGTTTTTACGTAACAGCTGGACTGCAGTCAGAGATGCGTGAACCGAAAAAAACACCGTGA", "aa": "MKQQKPKISFIAAMLIVIGSSIGAGIFFKSSTVLENSQASLVLAIFNWLVASVAVIAMALALIEIASVRNDNLSIISWVKVFNRRWLYHGCKNFMTYLYLPLTFFFMPLYFICSIQDGFRGLLGLETGAHFNTSVDWLIWLALALIITTYFLTIPPLYAKVGNIQNMVVSAVKFIPLVFVPIIGFIVAGTGNGELKNVKALVQPPQINGATASFTQLVQAGYGITRFTGIGAGMGSFISIAAIFFAYDGFYVTAGLQSEMREPKKTP", "aa_hexdigest": "ccea19e1dcd65445d1d7b1eb7f7c3e4b", "start_type": "ATG", "rbs_motif": "AAAA", "psc": { "uniref90_id": "UniRef90_UPI00244EE166", "query_cov": 0.9775280898876404, "subject_cov": 0.8642384105960265, "identity": 0.552, "score": 275.0, "evalue": 3.12e-89, "valid": false, "product": "amino acid permease", "uniref50_id": "UniRef50_UPI00244EE166" }, "pscc": { "uniref50_id": "UniRef50_UPI00244EE166", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00244EE166" ], "product": "amino acid permease" }, "genes": [], "id": "APKECPOBEN_679", "locus": "GCA002090215_00651" }, { "type": "cds", "sequence": "contig_1", "start": 363301, "stop": 363504, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTTTAGGTTTACTAATTACTACGCTGTTTTACCTTATCTTAGCAGTGGCACTTTCCATTAACGGTGGTTTGTTTAGTGGTATGGAAGAGTCCATGGGAAAGCTGTTTAACAATAAGCGTGCCGGGCAAATTGTCTTTGGTGTTGTTAACCTAATGATTGGCATAGGTGTCTTGGGTATTATTAACGGTTTTGCACTGTGA", "aa": "MFLGLLITTLFYLILAVALSINGGLFSGMEESMGKLFNNKRAGQIVFGVVNLMIGIGVLGIINGFAL", "aa_hexdigest": "33b2f0ba50e7efbad43f86d9b5ef9918", "start_type": "TTG", "rbs_motif": "AAAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7092.5383999999995, "isoelectric_point": 8.25416126251221 }, "id": "APKECPOBEN_680", "locus": "GCA002090215_00652" }, { "type": "cds", "sequence": "contig_1", "start": 363562, "stop": 363771, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCAGGGCAGATTGAACCCCAACAAGCCAGTGGTGGGGGTAATTTACTGTTTGGTACTTTCTTTGACTGTTCAAGTGCTGTTTACGGTAATAGGTGCTTTGGCTTACCTCCCGACAGTAGCGGATTACAAAAATTACGTTAACACTGAAATTGACAAGCTCAATTCGATGCAATGGTTGTATTCGTTTTCCGATTTAATGGCTACTTGA", "aa": "MQGRLNPNKPVVGVIYCLVLSLTVQVLFTVIGALAYLPTVADYKNYVNTEIDKLNSMQWLYSFSDLMAT", "aa_hexdigest": "e0149582a18217d9572dc2a154a50fd6", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7745.0167, "isoelectric_point": 5.916052436828613 }, "id": "APKECPOBEN_681", "locus": "GCA002090215_00653" }, { "type": "cds", "sequence": "contig_1", "start": 363898, "stop": 364191, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGTTAACTGCATTAGTGTTTTTGTAACTATCATTGAGCCCTTCATTAACCTCTTTTTACTGTTTGGTTATGATGAAACAGTGGCACACACAGTGCTGGGTAATGACTTCATTGAATTGAATGAATTAGTGATAGGACGGGTAATGCTGATAGTAGTGTTAGTTTTCTTTGCCATAATTAGCTTTCTCCCTGTTTATGTGGAGGATCAATACCACAAACGTAAGTTTGGTAGTTTAGCTAACTATCAGCAATATGTACAACAACATTTAGCACACTCCACCATTAACGGTTAA", "aa": "MVNCISVFVTIIEPFINLFLLFGYDETVAHTVLGNDFIELNELVIGRVMLIVVLVFFAIISFLPVYVEDQYHKRKFGSLANYQQYVQQHLAHSTING", "aa_hexdigest": "f9b3ac9992a53fa9f0deb599d6c69132", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11138.88329999999, "isoelectric_point": 5.32839412689209 }, "id": "APKECPOBEN_682", "locus": "GCA002090215_00654" }, { "type": "cds", "sequence": "contig_1", "start": 364247, "stop": 364741, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAGCATAGTCTTGTGGATATGCTTGACCATCCTGACCATAGCCGTAATAAGCATTGGGATCGGTGTAGTTATTAGGATCTACATAAGCGTTTGGGTCCTGATAAGCATTGGGATCTACATAGGCGTTAGGATCGGTGTAAGCGTTTGGGTCCTGATAAGCATTGGGATCGGCATAATACGCTTGGTTGGGATCTTGAGCATAGTCTTGTGGATATGCTTGACCATCCTGACCATAGCCGTAATAAGCCGCTGCATCAGGATAGGTATTCGGATCGGGGTAATACGCTTGGTTGGGATCAACTGCTTGGTCGGGGTCGTAATAAGTACTACCATCACCATAGTACTGCTGCTGATTGGGATCGTATGCAGTAGCTTGTTGGTCAGACTGATTTCAACCTGGTTTATTTATATCCATTTACTGTCTTTAAAAGGTGCCAACAGCAAAGCTGACACTATACCATTAATTTTAATTTATCTAAAAAAATGCAAATGA", "aa": "MSIVLWICLTILTIAVISIGIGVVIRIYISVWVLISIGIYIGVRIGVSVWVLISIGIGIIRLVGILSIVLWICLTILTIAVISRCIRIGIRIGVIRLVGINCLVGVVISTTITIVLLLIGIVCSSLLVRLISTWFIYIHLLSLKGANSKADTIPLILIYLKKCK", "aa_hexdigest": "ddbecde1683702dd2ef819485d510dea", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17842.236300000004, "isoelectric_point": 10.152690696716306 }, "id": "APKECPOBEN_683", "locus": "GCA002090215_00655" }, { "type": "cds", "sequence": "contig_1", "start": 365075, "stop": 365272, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCGCAAAGCGATTGATACTGTCAAGGAAAACAGCGCTAACTTGCAAATTATGAAGCAGAACTTTGAACGCCAACTCAAAGAACAGCAAACCCAACGGCTTACAAGTAATACTGACGCGGAAAAGATTGGTGCTAAGATCAACCAATTAGAGGAACGCATGCAACGTTTATCGCGCACTATGGAAAGTGTGGAGTGA", "aa": "MRKAIDTVKENSANLQIMKQNFERQLKEQQTQRLTSNTDAEKIGAKINQLEERMQRLSRTMESVE", "aa_hexdigest": "039a7f886538e5d6573fdcfaf0014f14", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7666.626500000002, "isoelectric_point": 9.297517585754395 }, "id": "APKECPOBEN_684", "locus": "GCA002090215_00656" }, { "type": "cds", "sequence": "contig_1", "start": 365363, "stop": 365542, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAATGTTTGAAAAGGTATTAATGATGAACTTACTGCGCAGCACCACTCCAGTCCAACCTCCAGTGCAGTACTTTACTCCTCAACCCCTTACAGCATCGCCACGTCCAGTGTATGAAGAACCGATCTCCGCTTCGTTCCGCCGTCGTGGTTACCGTGGTGATGAATTTTACGAATAA", "aa": "MSMFEKVLMMNLLRSTTPVQPPVQYFTPQPLTASPRPVYEEPISASFRRRGYRGDEFYE", "aa_hexdigest": "16f35960ec594689398f294edc8c290c", "start_type": "ATG", "rbs_motif": "AACAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6943.915500000001, "isoelectric_point": 8.140503501892091 }, "id": "APKECPOBEN_685", "locus": "GCA002090215_00657" }, { "type": "cds", "sequence": "contig_1", "start": 365562, "stop": 365975, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGATACTGACAAGAAGTTCCCCTTGCAACCAGTTTATGACACTGGGTTTGATGATGGGTATTTACAGCGTGATTATGAAAAGTGTTTAGAAAGTGCTGCTGCTAATGATGCACAAACAGTGGAACTACAAACCCAGCTGTTAGCAGAGATTAAAAACCTTGAAAACGAAATCAAGGCCCTAAAGGCGCAGGAATCACGCCAACCCGATCCGCACAACAATGCCCGGATTCAAAGCTTAGAAGCTAGCTTAAACCGTTTGGTCAATGAGTACAACAACTTTGAATTCCAAAAGAACTATATGGTTGATCGGGTAGCAGAGTTAAATAACAAAGCGCGTTTTTTTAAGGACGAATTAAAGCGTCTCCAACAAGAAAACGCCGCTTTTGTTAATTCCCGTTATGCCAATTGA", "aa": "MNDTDKKFPLQPVYDTGFDDGYLQRDYEKCLESAAANDAQTVELQTQLLAEIKNLENEIKALKAQESRQPDPHNNARIQSLEASLNRLVNEYNNFEFQKNYMVDRVAELNNKARFFKDELKRLQQENAAFVNSRYAN", "aa_hexdigest": "eea23d0c5bd79ca794dcf0ab6e0733ab", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15981.493999999984, "isoelectric_point": 4.97343463897705 }, "id": "APKECPOBEN_686", "locus": "GCA002090215_00658" }, { "type": "cds", "sequence": "contig_1", "start": 366216, "stop": 366338, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGGCATCGAGTATGAAGCCACATACCAGGACTTGGTGAGTGCGGATGCGGAGTTGCAAAACGTTTATGAAACAATTGCCCAAAACCAGGCCAACTTCCAAAAGCAGTGTGATGCTTATTGA", "aa": "MGIEYEATYQDLVSADAELQNVYETIAQNQANFQKQCDAY", "aa_hexdigest": "255ce307c715f42848a3b54350a7048e", "start_type": "GTG", "rbs_motif": "AAAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4576.8905, "isoelectric_point": 4.0500284194946286 }, "id": "APKECPOBEN_687", "locus": "GCA002090215_00659" }, { "type": "cds", "sequence": "contig_1", "start": 366387, "stop": 367283, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTTGATGAAGAATCGACACTTAAGGTGCGTTTAAATGACGCTGATTTTTACATTAACTCACGCTTAGCAGAATTAGATGATTTAACTAGCAAGATTAACGAACGTGATTTTGTTTCTAAAGAACAAGCTCAAGACGTTAAAGCTAGTTTAGCTAACCTCACTAAGGAAAAAGAACGTTTGAGCGCTGAGAAGGATAGCTTTGAAAGGTTAAGGAACACCGCTTTAAACGACATTAACCGAATGGAGCAGGAAAACGCGTTGTTTGCTAAACACTTAGAACAGCAACAATACGAGTTTGAAAGAAAGCAACAGGAAAGCTTATTAAAGCTAGAAACAGAGCATAAACAGCTCCAAAAACGAATTGGTGAGTTCAAAATCGAGAGTGAAGCTAAAAGTGAAGCGTTACTGATACAAGAACGTGAACTGTTGGAAAAACGCCGGGAAATTGATGATTTGTTAACCCAAGCCAGCTTGGAGTATGAACAACAACGCCGGACGAATCAGGTCTTAAAGGAAAAGCACCGTCAAGTCCAACAACACTTCCAAAACCTCGTGCATGCTAAAAAGAAATTGGATCAAAAGCGCCATTATTTAGCGGAACAAAAGCGGATTGATGAAGAACAGATTTTTAAGTTAAAGGAAAAGATTGCCACTGAACGCCGCGAATTGGAGAAGCTTTATCTAGTTAAAAAACAAAAGCAAGACCAAAAGGAAAACGATTTACTGATTTTTGAAAAACAGTTAAGGCAGTACCAAGCTGACTTTGAAAACGAAATAGAAGAGAAGCAAAACGAGCTGTTTGCATCCCAAAAATCACTCCAAAAATCGTTTACACAACTCAAAAATAAGGAAGCGGAATTAAACCAAAAGGCACAAAAGATTGCAGAGGATTGA", "aa": "MVDEESTLKVRLNDADFYINSRLAELDDLTSKINERDFVSKEQAQDVKASLANLTKEKERLSAEKDSFERLRNTALNDINRMEQENALFAKHLEQQQYEFERKQQESLLKLETEHKQLQKRIGEFKIESEAKSEALLIQERELLEKRREIDDLLTQASLEYEQQRRTNQVLKEKHRQVQQHFQNLVHAKKKLDQKRHYLAEQKRIDEEQIFKLKEKIATERRELEKLYLVKKQKQDQKENDLLIFEKQLRQYQADFENEIEEKQNELFASQKSLQKSFTQLKNKEAELNQKAQKIAED", "aa_hexdigest": "714b1d7b4bae9673bbb6068a4275bdce", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 35848.88910000005, "isoelectric_point": 6.043087577819825 }, "id": "APKECPOBEN_688", "locus": "GCA002090215_00660" }, { "type": "cds", "sequence": "contig_1", "start": 367377, "stop": 370445, "strand": "+", "frame": 3, "gene": "hmw2", "product": "Cytadherence high molecular weight protein 2", "db_xrefs": [ "COG:COG1196", "COG:D", "GO:0020035", "SO:0001217", "UniRef:UniRef50_P75471", "UniRef:UniRef90_P75471" ], "nt": "TTGGAAGCACGTACGCAGTTTGACAACCGTGTCAGTTTGCTCTCAGCACGCTTTAAACAAAAACAAGCAGAATTAGTTAAACAAAAACAAAGCTTGGAACAACTCACCGCTGCCTTTAATAAAGAGCAGGAAGCGGTAGAGCGTGATTGGAAGGATCGCTTAGCTAACTTAGAAAAGCAAAAGGAAATGCTGGGGGATAAGGTACACCAGTTTGATGAAAATTCGCTCAATATTTCCAAAAAGCTAGCGGAGCGGGAGCTAGCGATTAAATTTAAAGAAAAGGAGCTGGAGGCTGCACAAAAACAGCTAAGTTTAGACAACAACAATAACGCTGGGTTAAAGTTGCAGTTAGATAAATTGAGCGAATCACTCAAAACGGAACGCCTGGAATTAGAAGCATCTAAAGAACGTATTTTGGATTTTTACGATGAATCATCACGCCGAATTGCAGATTATGAAAGCGATTTACAAGCACGCTTAGCTGAGGTTAAGACACTAGAAAAAAACCAGCAGGAAACAGCGGCTAAAAGTGAGCGCGAACTCAAAGTAGCCTTAGAAAAGTTAAACCAAGCAAAAAAGGCTTTTTTACAAATTCGCAAACAGCAATTGCTCGAAATTGCTTCTGTAAAACAACAACTAGCACAAAAAGCTAATTTGTTGAAAAACCAACAAGCAGAGCTAGATAAGCAAACAGAAGAGTTAGAAGCAGCTTTTTTAGAGCAAGATACGGACAAAAAGGAGTTGGAAAAAGCACTCCACAGCGTTAAGTCCAAGCAAGAATTATTAGAGCGCGAACGCAGTTTTTTACTGCAAAAACAACGTGAATTTGCCGAACATGTAGCCGGCTTTAAGCGCCAAGTGCACTTTAAAACAACGCAAATGCAGCGTTTAAGTGAGTTCAACAAACAACAACAAAGTGAACAGATAAAGCGCGAAACGGAGCTCAAAATTGCTTTTGCTGATCTAAAAAAGGATTACCAGCTGTTTGAATTGCAAAAAAACCAGGAGTTCCAGCAAATCGAACAAAAGCACAAAGAACTAGAACTATTAGCACAAAAACAGGCCGAACTCAAACAGGAGTTAGAGCAAAAAGCAACAGCTTTAGCTAGCCAAGATCAAGACACAGTACAGGCCAAGCTAGATTTAGCGCGTCAACAGCATGAATTGGAATTGAGGCAAAATGCCTTTAACCAAGCTAGTCTTTCGCTCAACAAACAACGCGAACAGCTCACAAACCAAGTCAAGGTATTGCACGGTGAGTTGAAAAAACGCCATGAAAAGCTAACTTTAAAGGATCGTTTACTAGCTGAAAAGGAAAAGGATCAGCACAAAAAAGATGCAGAAATTAACCAACGCTTTAAGCAGTTTGAAAATGAATACGCTGATTTTGACCAAGCCAAAAAGCGCGAATTGCAAGAGTTAAACCAAATCCGTCGAAACTTGGAGCAGAGCAATGCATCACTGCTAAAGAAGCGCAACCAACTAACGCTTGATTTTGCTTTACTACGTAAAGTGCAACACAATACGCAAACTAACCGTGTCCAACTCAACACGCAAATTAAGGAGTTTTTACTAGAGAAGAAGAACTTTCAAAAGGCGAGTGATGAAGCCGCTTTACAAAAAGCACTGTTAATTAAACGCTTACGTAGTTTTGCTTCTAAATTACAGTTGCAACGCGAAGCTTTAGCGATTCAAAAACTGGAATTTGATAAGCGTGATGAACAACAAAAGAGTGAGATTAACAACGCTAAGTTACAGTTAGAGCAGTTCAAACTAGAAAAGCAGAACTTTGATGAAGCGAAACAAAAGCAGTTAATTGAGTTTAAGGATCAGTGCCAACGACTTGATGTTGAAAAACGTCTGTTAAAGCAAAAGTTAGTGCAGCTCAAAAACCTATCTAAGAGCTATCTTACTTACAAAAACCGTGCTGATCTGTCCCAACAACAGCTGCAACACAAGTATGCTAACTTACTGGAGTTGAAGGAAAAGCTGCAAACCGCTAAGCGTGCCTTGGACAAAAAACACCGTGCCATTTATGGCAAAATGGCACAGTTTGTCAGTGAGCTCCGCCAAGAAAAGAAGCAGCTTTTAAGTGCGCAAAAACAAGTTGATGACAAGTCACGTTTACTGGAACAAAACCAACGCCATTTACAAAACCTTTCCAGTGAAACCAAAAAGAAACGCCAGTCTTTAGAGCATGACATTAATAAGTTTGATCAAAGACGCAAGGAAGCGGTTTCTTCCATTTTAAACTCGCACAAAAAACTCAAGCAAAAAGAGGGAGAATTGCAAGGCATTTTGCAAAAGCTTAGCTTGAAAAAAACACAAATTGAACAAGAGTTTTCCAAGCTTTATCAGCAACGCGAAAAGTTAGATCGCCAACGCACTACGCTCTCTAAACTCCACAGAGAGCTAAAAGCCCAAAATGAAGCTACGGCACACAAAAATCGTGAAGTTTTAGAGATTGAGAACTATTACAAGAAGGAGTTGCAACGACTCACCACGGAAAAGAGTGAGTTTGATAACAACAAAAATCGCTTGTTTGAATACTTCCGTAAAATTCGCAATGAAATTGAAAAGAAGGAAGCACACATTAAAACCGTTTTAGAAGAAACACAAAAAAAGCGTCACCTAGTCGAAACGGAAGCGGTCAAACTACATTTACAAAAGCAATCTATCATTTCTAAGGGTCAAGAACTTAAAGAAATCAAGGAACGGGTTAGCCGTGACATTAGCCACACCAACAAACAACGCGAAGAGTTAAACAGCTTATTGCACCAAAACAAACTGTTGCAAAAGAATTTGGCTGAGCGTGAACGTGAAATTAATAATAAGGATTCACTGTTAACCCAAAAGATTCAAACAGCTAAACAAAAGTTAAGCGAAAAAGAAGCACGCATCTTAAAGCTGTTGGAAAAAATGCGTGCGGTAGAACAGCAATACCAAGCGGAAATTACCCGTTTAAAGACACGTAATGCTGATTTGGAGAAGAATGACAATAAGCACTTATTTCCACCGTTATTTAAAATTAACGGTAATGATATGAACTATCCTTACCCATATCCTTGA", "aa": "MEARTQFDNRVSLLSARFKQKQAELVKQKQSLEQLTAAFNKEQEAVERDWKDRLANLEKQKEMLGDKVHQFDENSLNISKKLAERELAIKFKEKELEAAQKQLSLDNNNNAGLKLQLDKLSESLKTERLELEASKERILDFYDESSRRIADYESDLQARLAEVKTLEKNQQETAAKSERELKVALEKLNQAKKAFLQIRKQQLLEIASVKQQLAQKANLLKNQQAELDKQTEELEAAFLEQDTDKKELEKALHSVKSKQELLERERSFLLQKQREFAEHVAGFKRQVHFKTTQMQRLSEFNKQQQSEQIKRETELKIAFADLKKDYQLFELQKNQEFQQIEQKHKELELLAQKQAELKQELEQKATALASQDQDTVQAKLDLARQQHELELRQNAFNQASLSLNKQREQLTNQVKVLHGELKKRHEKLTLKDRLLAEKEKDQHKKDAEINQRFKQFENEYADFDQAKKRELQELNQIRRNLEQSNASLLKKRNQLTLDFALLRKVQHNTQTNRVQLNTQIKEFLLEKKNFQKASDEAALQKALLIKRLRSFASKLQLQREALAIQKLEFDKRDEQQKSEINNAKLQLEQFKLEKQNFDEAKQKQLIEFKDQCQRLDVEKRLLKQKLVQLKNLSKSYLTYKNRADLSQQQLQHKYANLLELKEKLQTAKRALDKKHRAIYGKMAQFVSELRQEKKQLLSAQKQVDDKSRLLEQNQRHLQNLSSETKKKRQSLEHDINKFDQRRKEAVSSILNSHKKLKQKEGELQGILQKLSLKKTQIEQEFSKLYQQREKLDRQRTTLSKLHRELKAQNEATAHKNREVLEIENYYKKELQRLTTEKSEFDNNKNRLFEYFRKIRNEIEKKEAHIKTVLEETQKKRHLVETEAVKLHLQKQSIISKGQELKEIKERVSRDISHTNKQREELNSLLHQNKLLQKNLAEREREINNKDSLLTQKIQTAKQKLSEKEARILKLLEKMRAVEQQYQAEITRLKTRNADLEKNDNKHLFPPLFKINGNDMNYPYPYP", "aa_hexdigest": "d72fdf9c34d77bac75b9ec52f6dac826", "start_type": "TTG", "rbs_motif": "AACAA", "genes": [ "hmw2" ], "truncated": "both", "pseudogene": { "start": 367077, "stop": 370748, "observations": { "mutation": [ 367281, 370443 ], "stop": [ 367281, 370443 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75471", "query_cov": 1.0, "subject_cov": 0.5621562156215621, "identity": 0.986, "score": 1640.0, "evalue": 0.0, "gene_start": 1, "gene_end": 1022, "reference_start": 606, "reference_end": 1627, "reference_sequence": "MNDTDKKFPLQPVYDTGFDDGYLQRDYEKCLESAAANDAQTVELQTQLLAEIKNLENEIKALKAQESRQPDPHNNARIQSLEASLNRLVNEYNNFEFQKNYMVDRVAELNNKARFFKDELKRLQQENAAFVNSRYANWADFQSNYQLKLDQFQALIDQQNQTIKQLNEQIAANQGLIDQNVQRLQQNHSLDQQERDALLYEVDHLYNELYELENQKRLVGIEYEATYQDLVSADAELQNVYETIAQNQANFQKQCDAYWAQLKQVEQQIQTTKQELVDEESTLKVRLNDADFYINSRLAELDDLTSKINERDFVSKEQAQDVKASLANLTKEKERLSAEKDSFERLRNTALNDINRMEQENALFAKHLEQQQYEFERKQQESLLKLETEHKQLQKRIGEFKIESEAKSEALLIQERELLEKRREIDDLLTQASLEYEQQRRTNQVLKEKHRQVQQHFQNLVHAKKKLDQKRHYLAEQKRIDEEQIFKLKEKIATERRELEKLYLVKKQKQDQKENDLLIFEKQLRQYQADFENEIEEKQNELFASQKSLQKSFTQLKNKEAELNQKAQKIAEDWAHLKQNKHHHADLEIFLEGEFNHLQQEKHKLLEARTQFDNRVSLLSARFKQKQAELVKQKQSLEQLTAAFNKEQEAVERDWKDRLANLEKQKEMLGDKVHQFDENSLNISKKLAERELAIKFKEKELEAAQKQLSLDNNNNAGLKLQLDKLSESLKTERLELEASKERILDFYDESSRRIADYESDLQARLAEVKTLEKNQQETAAKSERELKVALEKLNQAKKAFLQIRKQQLLEIASVKQQLAQKANLLKNQQAELDKQTEELEAAFLEQDTDKKELEKALHSVKSKQELLERERSFLLQKQREFAEHVAGFKRQVHFKTTQMQRLSEFNKQQQSEQIKRETELKIAFADLKKDYQLFELQKNQEFQQIEQKHKELELLAQKQAELKQELEQKATALASQDQDTVQAKLDLARQQHELELRQNAFNQASLSLNKQREQLTNQVKVLHGELKKRHEKLTLKDRLLAEKEKDQHKKDAEINQRFKQFENEYADFDQAKKRELQELNQIRRNLEQSNASLLKKRNQLTLDFALLRKVQHNTQTNRVQLNTQIKEFLLEKKNFQKASDEAALQKALLIKRLRSFASKLQLQREALAIQKLEFDKRDEQQKSEINNAKLQLEQFKLEKQNFDEAKQKQLIEFKDQCQRLDVEKRLLKQKLVQLKNLSKSYLTYKNRADLSQQQLQHKYANLLELKEKLQTAKRALDKKHRAIYGKMAQFVSELRQEKKQLLSAQKQVDDKSRLLEQNQRHLQNLSSETKKKRQSLEHDINKFDQRRKEAVSSILNSHKKLKQKEGELQGILQKLSLKKTQIEQEFSKLYQQREKLDRQRTTLSKLHRELKAQNEATAHKNREVLEIENYYKKELQRLTTEKSEFDNNKNRLFEYFRKIRNEIEKKEAHIKTVLEETQKKRHLVETEAVKLHLQKQSIISKGQELKEIKERVSRDISHTNKQREELNSLLHQNKLLQKNLAEREREINNKDSLLTQKIQTAKQKLSEKEARILKLLEKMRAVEQQYQAEITRLKTRNADLEKNDNKHLFPPLFKINGNDMNYPYPYPWFYPQQKQEDSSNQIRHLFEQQLQFMQQRYENELTELRRQRALLEKKLDQIQLESQLSAKKNDFEKVEQMMQKLLEKTEQKLSAFDQKINALAEQINTQKAEHADSEKQQLLLRIEQLEKQNLAQAVQTPQPVQPVVQAPAVVPQVIQPQVVQSQPAFLATQQSISKQQQIAQLNAEINSIKKLIAQKAAK" }, "paralog": false, "identity": 0.9926410466067048, "score": 2164.0, "evalue": 0.0, "description": "Internal stop codon at 367281, 370443. Nonsense mutation around 367281, 370443.", "psc": { "uniref90_id": "UniRef90_P75471", "gene": "hmw2", "product": "Cytadherence high molecular weight protein 2", "uniref50_id": "UniRef50_P75471", "cog_id": "COG1196", "cog_category": "D", "go_ids": [ "GO:0020035" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75471", "UniRef:UniRef50_P75471", "COG:COG1196", "COG:D", "GO:0020035" ] }, "pscc": { "uniref50_id": "UniRef50_P75471", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75471" ], "product": "Cytadherence high molecular weight protein 2" } }, "id": "APKECPOBEN_689", "locus": "GCA002090215_00661" }, { "type": "cds", "sequence": "contig_1", "start": 370518, "stop": 371018, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAACAGCGTTACGAAAACGAATTAACTGAGTTGCGTCGCCAACGTGCTTTATTGGAAAAAAAACTAGACCAAATTCAACTCGAATCACAGTTAAGCGCGAAGAAGAACGACTTTGAAAAGGTTGAGCAAATGATGCAAAAGTTGTTGGAAAAAACTGAGCAAAAACTCAGTGCCTTTGACCAAAAAATTAATGCTTTGGCAGAACAGATTAACACACAAAAAGCAGAGCACGCTGACAGTGAAAAGCAACAGTTGTTACTAAGGATTGAACAGTTAGAAAAGCAAAACCTAGCTCAAGCTGTTCAAACACCCCAACCAGTACAACCAGTGGTACAAGCACCAGCTGTAGTACCCCAAGTGATCCAACCACAGGTAGTGCAGTCCCAACCAGCTTTTTTAGCAACGCAGCAAAGTATTTCTAAACAGCAACAAATCGCGCAATTAAACGCTGAGATCAATAGTATTAAAAAGCTAATTGCCCAAAAAGCAGCTAAATAA", "aa": "MQQRYENELTELRRQRALLEKKLDQIQLESQLSAKKNDFEKVEQMMQKLLEKTEQKLSAFDQKINALAEQINTQKAEHADSEKQQLLLRIEQLEKQNLAQAVQTPQPVQPVVQAPAVVPQVIQPQVVQSQPAFLATQQSISKQQQIAQLNAEINSIKKLIAQKAAK", "aa_hexdigest": "92065eea48fde9f1034a1024184f916b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18971.563099999985, "isoelectric_point": 9.097601127624515 }, "id": "APKECPOBEN_690", "locus": "GCA002090215_00662" }, { "type": "cds", "sequence": "contig_1", "start": 371033, "stop": 372106, "strand": "+", "frame": 2, "gene": null, "product": "putative MG218-1-like protein", "db_xrefs": [ "RefSeq:WP_010874667.1", "SO:0001217", "UniParc:UPI000013949B", "UniRef:UniRef100_P75470", "UniRef:UniRef50_Q9ZB78", "UniRef:UniRef90_P75470" ], "nt": "ATGACTAATGATTACCAACAATTAAATTATTTAGTGGAAACTGATGATGAAGCTGACATCATCATAGCTAACCTCGTCAAACAACTCAATGAATTGAAGGAAATTCTGCTTTCATTGGACAATCAAGATTTAGAAATTAATCAGGTTAACCATCGCACCACAGTGCACAATACTAGTTCTAACACTTCTAATAATAGCACTAGCAATAATATTGCTGATGGTAATTACAACAGCAATTTCTTTCACAACTTTAGTAAAGAAACGCTGCAAACACAAGCAAAACGTGGCTTTCTTTTACTAGAACGCTGTTCCTTGGTAGGTCTACAACAATTGGAGTTGGAATACCTCAACGTATTGGGTCGTAGCTTTGAATCACACCAAGACAAAATTAACTTTTTGGTCAATTTACGTGATTTAACTAACGATCACTTGTTGGACACGGAAAAGATTGTCAGTACCCTAGAGCAGATCTTTAACGTTATTGGTGGCACGGAATACACCCCGATTCTCAATTCCTTTTTTAACCAATCACTTAACGATCCTGACCCAATTCAGCGCGAAATTGGACTCAAACAGTTTGTGGCTAACTTACGCCAACGCTTTAAAACGCTATTGCAAAAAACTAACAACTCAATTCAACAGTTAGAAGCGGAAATTCAAATTCCAACTACTCATATTAAGAGTGATGAGGTAATGTTTGGCCCACCGGACATGAACGAACGCTTAGTGTTAAATGACAGTGAAACCGATGCCATTCTACGCTCAATTGAAGCGGAGTTGGAAAGCGCTTTACAAAATAAAAAGCAGGTAGTGGTTACAGCAGTCCCACCAATAGCTGCTAACTTAGCTACCGATAGTATTAGCCAAGAAACTTTAGAACACAACCTTAATAACACTGAAACGGTTAACACCACTACTGTCACGGTAACATCAGCTAGTGAAACTCAAGTGCGTAAACCACAGATTTCATTACGCCCCATTTTTCAAGGCAACTTTCCCAAACGCTTAAGTAGAGAAGACATCCAGCGCTATGCCCACCAGTTACAGGAATTAGAACAAAGTAATGAAGGATAG", "aa": "MTNDYQQLNYLVETDDEADIIIANLVKQLNELKEILLSLDNQDLEINQVNHRTTVHNTSSNTSNNSTSNNIADGNYNSNFFHNFSKETLQTQAKRGFLLLERCSLVGLQQLELEYLNVLGRSFESHQDKINFLVNLRDLTNDHLLDTEKIVSTLEQIFNVIGGTEYTPILNSFFNQSLNDPDPIQREIGLKQFVANLRQRFKTLLQKTNNSIQQLEAEIQIPTTHIKSDEVMFGPPDMNERLVLNDSETDAILRSIEAELESALQNKKQVVVTAVPPIAANLATDSISQETLEHNLNNTETVNTTTVTVTSASETQVRKPQISLRPIFQGNFPKRLSREDIQRYAHQLQELEQSNEG", "aa_hexdigest": "8c0c77514e8df669bff7b04f58c9c2c2", "start_type": "ATG", "rbs_motif": "AATAA", "ups": { "uniparc_id": "UPI000013949B", "ncbi_nrp_id": "WP_010874667.1", "uniref100_id": "UniRef100_P75470", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013949B", "RefSeq:WP_010874667.1", "UniRef:UniRef100_P75470" ] }, "ips": { "uniref100_id": "UniRef100_P75470", "uniref90_id": "UniRef90_P75470" }, "psc": { "uniref90_id": "UniRef90_P75470", "product": "Uncharacterized protein MG218.1 homolog", "uniref50_id": "UniRef50_Q9ZB78" }, "pscc": { "uniref50_id": "UniRef50_Q9ZB78", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9ZB78" ], "product": "Uncharacterized protein MG218.1" }, "genes": [], "id": "APKECPOBEN_691", "locus": "GCA002090215_00663" }, { "type": "cds", "sequence": "contig_1", "start": 372096, "stop": 372650, "strand": "+", "frame": 3, "gene": null, "product": "putative protein MPN_312", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75469", "UniRef:UniRef90_P75469" ], "nt": "ATGAAGGATAGTGCACTAACACTAAAACGGGTACGAATTGGAAAATTTTCCGAGTCAATGGTTGAAGAACGACCAACACTCAACCTGTTCGAAAAGGTCGAATTCAACCCAGTACCGACTGCTTTAGTGGATCAATTGCCAACTGAACCACTTGTTGAAGCAACTCTTTTAGAAAAGGAAGCAATTACTTTTGTCGATACTTACGCAACTTCGGAAGCTCATGATCAAATAGCTACCTTTGTTTTAGAACAATCAATGGAAACGGAGGTGGTTGAAAAAGAAGCGATTGAAACAGCAATAGTTGCACCAGCACCAGACTTAGTTGAAGAGAAAGCTGTTTTAGTGGAAGAGGTTTTAGTTGAACCAACTGCAACGGAAGCAGTTACAACTGAAGAAAATCAAGTTTCAACTACATCCGTAACTAAGATTAAAACTAAGCGCAGCAATACTAAAAAGGTGACATCAGAAACACTTGTGGCTTCTAAAAGTGTGAAAACTAAAAAGTTAATTACCCCTAACCGGGTAAGTTCGGGCAACGTTAATATAACACTTTGA", "aa": "MKDSALTLKRVRIGKFSESMVEERPTLNLFEKVEFNPVPTALVDQLPTEPLVEATLLEKEAITFVDTYATSEAHDQIATFVLEQSMETEVVEKEAIETAIVAPAPDLVEEKAVLVEEVLVEPTATEAVTTEENQVSTTSVTKIKTKRSNTKKVTSETLVASKSVKTKKLITPNRVSSGNVNITL", "aa_hexdigest": "5b386ea693dd4068c2a82f5e25a6b8e8", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75469", "query_cov": 1.0, "subject_cov": 0.8440366972477065, "identity": 1.0, "score": 270.0, "evalue": 1.55e-89, "valid": true, "product": "Uncharacterized protein MPN_312", "uniref50_id": "UniRef50_P75469" }, "pscc": { "uniref50_id": "UniRef50_P75469", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75469" ], "product": "Uncharacterized protein MPN_312" }, "genes": [], "id": "APKECPOBEN_692", "locus": "GCA002090215_00664" }, { "type": "cds", "sequence": "contig_1", "start": 372835, "stop": 373029, "strand": "-", "frame": 1, "gene": null, "product": "MPN313 family protein", "db_xrefs": [ "GO:0016020", "SO:0001217", "UniParc:UPI0001DD6525", "UniRef:UniRef100_P75468", "UniRef:UniRef50_Q49403", "UniRef:UniRef90_P75468" ], "nt": "ATGATTGTAAACGCCTTTAGTACGTTAATTAATACCGCGTTAGACATCAGCGAAGCTGCTAACGCTAATAATGCCAACGGCAATGGCAGCAGTTATAGCTATAAAAGAAGGAATTCACAAAAAGACTACTTTAGTACCGGTCGGTTCCGATTAGGTTTAACCCCTGGTAAATCGTCCTATTCCTTTCCCGTTTAA", "aa": "MIVNAFSTLINTALDISEAANANNANGNGSSYSYKRRNSQKDYFSTGRFRLGLTPGKSSYSFPV", "aa_hexdigest": "faaba2bcf9aacc473b9113f13bb76ee0", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD6525", "uniref100_id": "UniRef100_P75468", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD6525", "UniRef:UniRef100_P75468" ] }, "ips": { "uniref100_id": "UniRef100_P75468", "uniref90_id": "UniRef90_P75468" }, "psc": { "uniref90_id": "UniRef90_P75468", "product": "MPN313 family protein", "uniref50_id": "UniRef50_Q49403", "go_ids": [ "GO:0016020" ] }, "pscc": { "uniref50_id": "UniRef50_Q49403", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49403" ], "product": "Uncharacterized protein MG220" }, "genes": [], "id": "APKECPOBEN_693", "locus": "GCA002090215_00665" }, { "type": "tRNA", "sequence": "contig_1", "start": 373177, "stop": 373253, "strand": "+", "gene": "trnR", "product": "tRNA-Arg(gcg)", "amino_acid": "Arg", "anti_codon": "gcg", "score": 65.2, "nt": "GTCATCATAGCTCAATAGGACAGAGTATCAGCTTGCGGAGCTGAGGGTTACAGGTTCGATTCCTGTTGGTGACGCCA", "db_xrefs": [ "SO:0001036" ], "anti_codon_pos": [ 373211, 373213 ], "id": "APKECPOBEN_9", "locus": "GCA002090215_00666" }, { "type": "cds", "sequence": "contig_1", "start": 373508, "stop": 373933, "strand": "+", "frame": 2, "gene": "mraZ", "product": "division/cell wall cluster transcriptional repressor MraZ", "db_xrefs": [ "COG:COG2001", "COG:J", "GO:0000976", "GO:0003700", "GO:0005737", "GO:0006355", "GO:0009295", "GO:2000143", "RefSeq:WP_010874670.1", "SO:0001217", "UniParc:UPI000012F547", "UniRef:UniRef100_P75467", "UniRef:UniRef50_P47463", "UniRef:UniRef90_P75467" ], "nt": "ATGCTTTTAGGAACCTTCAACATTACCCTTGATGCAAAAAACAGGATCAGTCTGCCAGCGAAGCTGCGGGCTTTCTTTGAAGGTAGCATTGTTATCAACCGTGGCTTTGAAAACTGCTTGGAAGTGCGTAAACCACAAGACTTCCAAAAGTACTTTGAGCAGTTCAACAGCTTTCCCTCCACCCAAAAAGACACTAGAACACTCAAGCGCTTAATTTTTGCCAACGCTAACTTTGTGGATGTAGATACAGCGGGACGGGTTTTAATTCCCAACAATCTGATTAACGATGCCAAGCTAGACAAGGAAATTGTTTTAATTGGCCAGTTTGACCACTTAGAGATTTGGGACAAGAAGCTGTATGAGGACTACCTAGCCAACTCTGAGTCGTTGGAAACGGTAGCGGAAAGGATGAAAGATGTTAAGTAA", "aa": "MLLGTFNITLDAKNRISLPAKLRAFFEGSIVINRGFENCLEVRKPQDFQKYFEQFNSFPSTQKDTRTLKRLIFANANFVDVDTAGRVLIPNNLINDAKLDKEIVLIGQFDHLEIWDKKLYEDYLANSESLETVAERMKDVK", "aa_hexdigest": "8b2975df80e77adad7ccac97fe29d2f1", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000012F547", "ncbi_nrp_id": "WP_010874670.1", "uniref100_id": "UniRef100_P75467", "db_xrefs": [ "SO:0001217", "UniParc:UPI000012F547", "RefSeq:WP_010874670.1", "UniRef:UniRef100_P75467" ] }, "ips": { "uniref100_id": "UniRef100_P75467", "uniref90_id": "UniRef90_P75467" }, "psc": { "uniref90_id": "UniRef90_P75467", "gene": "mraZ", "product": "division/cell wall cluster transcriptional repressor MraZ", "uniref50_id": "UniRef50_P47463", "cog_id": "COG2001", "cog_category": "J", "go_ids": [ "GO:0000976", "GO:0003700", "GO:0005737", "GO:0006355", "GO:0009295", "GO:2000143" ] }, "pscc": { "uniref50_id": "UniRef50_P47463", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47463" ], "product": "Transcriptional regulator MraZ" }, "genes": [ "mraZ" ], "id": "APKECPOBEN_694", "locus": "GCA002090215_00667" }, { "type": "cds", "sequence": "contig_1", "start": 373923, "stop": 374849, "strand": "+", "frame": 3, "gene": "rsmH", "product": "16S rRNA (cytosine(1402)-N(4))-methyltransferase RsmH", "db_xrefs": [ "COG:COG0275", "COG:J", "EC:2.1.1.199", "GO:0005737", "GO:0016434", "GO:0070475", "GO:0071424", "RefSeq:WP_010874671.1", "SO:0001217", "UniParc:UPI000012F4EE", "UniRef:UniRef100_P75466", "UniRef:UniRef50_P47464", "UniRef:UniRef90_P75466" ], "nt": "ATGTTAAGTAATCAACCCCACAAAAGTGTCTTACTTGATGAAGTCATCCACAACCTCAACATTAAAGGTGATGGTAACTATTTAGATCTTACTGTTGGTTTTGGCGGTCATAGTCAGCACATTTTAGAGCAGCTTACCACTGGCACTTTAACTGGCAATGACATGGATAAGGACAGCATTACCTTTTGTACCGAGCTGTTCAAAGACAAAAAGAACGTTGTTTTAGTCCATGATAACTTCGCTAACTTTTTTAACCACTTAAAGCAGCTCAAAGTAACGAAGTTTGACGGCATCTTAATTGATTTAGGGGTTTCGAGCTACCAGCTAGATAAACCAGAACGCGGTTTTAGTTTTAAACACGCCGGTCCATTTGACATGCGCATGCACCAAAGCGATCGCGTACCAACTGCTTTAGATATCTTGGAGAGGTTGTCTGAGGAAGAATTGACACATGTTTTAAAGAAGTTTGGTGAAATAGCCCACCCCAAACCAATCGCCAAGGCCTTAAAAACACTCATTAATAACACTAAAAATCCAACTACGGTAGAGGTAGCGGAAACAGTTAAGGCAGCTGCTAGCAACTTTGAAAAGTACAAATCACGCAATTACTTAGCTAAAGTTTTCCAAGCCATTCGCATTTACCTCAACCGTGAACTGGAAAGTTTGGAAATTGTGCTGCAACACATTCCCAAGCTGTTGAACAATAAGGGTCGTTTTCTAGTGATTGTCTTCCATTCACTCGAGGAAAAATTGGTGCGGAACTACATTTTTAAACTTACTCACTTTGTCCAACCCCCTGAACTACCCGTTAAATTAACCCCACCGTTTGAACTCATTACTAAAAAACCCATTTTGCCAACTGAACAAGAAATTAAAACGAACCCCCGTGTTCGAAGTGCCAAACTGTTTGTCATTGAAAAGAAATAA", "aa": "MLSNQPHKSVLLDEVIHNLNIKGDGNYLDLTVGFGGHSQHILEQLTTGTLTGNDMDKDSITFCTELFKDKKNVVLVHDNFANFFNHLKQLKVTKFDGILIDLGVSSYQLDKPERGFSFKHAGPFDMRMHQSDRVPTALDILERLSEEELTHVLKKFGEIAHPKPIAKALKTLINNTKNPTTVEVAETVKAAASNFEKYKSRNYLAKVFQAIRIYLNRELESLEIVLQHIPKLLNNKGRFLVIVFHSLEEKLVRNYIFKLTHFVQPPELPVKLTPPFELITKKPILPTEQEIKTNPRVRSAKLFVIEKK", "aa_hexdigest": "fcff818c2d37528332f59242bc11609f", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000012F4EE", "ncbi_nrp_id": "WP_010874671.1", "uniref100_id": "UniRef100_P75466", "db_xrefs": [ "SO:0001217", "UniParc:UPI000012F4EE", "RefSeq:WP_010874671.1", "UniRef:UniRef100_P75466" ] }, "ips": { "uniref100_id": "UniRef100_P75466", "uniref90_id": "UniRef90_P75466" }, "psc": { "uniref90_id": "UniRef90_P75466", "gene": "rsmH", "product": "16S rRNA (cytosine(1402)-N(4))-methyltransferase RsmH", "ec_ids": [ "2.1.1.199" ], "uniref50_id": "UniRef50_P47464", "cog_id": "COG0275", "cog_category": "J", "go_ids": [ "GO:0005737", "GO:0016434", "GO:0070475", "GO:0071424" ] }, "pscc": { "uniref50_id": "UniRef50_P47464", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47464" ], "product": "Ribosomal RNA small subunit methyltransferase H" }, "genes": [ "rsmH" ], "id": "APKECPOBEN_695", "locus": "GCA002090215_00668" }, { "type": "cds", "sequence": "contig_1", "start": 374852, "stop": 375967, "strand": "+", "frame": 2, "gene": null, "product": "MPN316 family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47465", "UniRef:UniRef90_P75465" ], "nt": "ATGTATAACCTCAAAAACATCTACGCCTCCATTACCCTTTACCCCCATGAAATTAACTTGGTGGTATCGGACAACAACAATCAGTTTCATGTCTTGTATGAAAACTCGATTGCTAACGATGAGCTTTACACGCACGCGGGCATTACTAATAAGGCAAAGTTCAAGGTAGTTTTAAACCAGCTCATTAACAATGCAAACGATTATTTGGGTTTTAAACTCGAAAAAGTAATTGTGGTTTTAGCGGAGTTAGTTGATGATCTTGAAATTAACCGCTTTAAAACAGATGTCTTTTTCACTGGTTATGACTTTAACCACCAGGACATGTTGAGCAGTGAAAAGAACCGCTTCTTGCTCAAAAACCGTCATATCAACCCCAATGAAGTTTTGGACTTAGTGGCTTTAAACTACCGTGATTTAACCACTGATAAAATTAGCAAAAACTTTAAGTACAACTGCAGTTATCGGGCAAATGTAATTTATTACACCACTAAAAACTCACTAGTCAAGGAGCTCAAACCGTTTTTAAAGCGCAATATTATTGTCAAAATTGACAAAATTGTCACCCACCACATGGTGTTAGCCCACTCAATTAAACAAATCAACAAAAACAACCTCTTTGTGTATTTAGGGGAACACACTACTGACCTCATGTTGTTTATGGACAATGCCTTAGTTGATTTAGTAAGTGAACCCTTTGGTCGGGTTAACTTCTTGGAATCTGACAATGACAGTGAAAACAAAGCGTTGTTGGAATTCCTAGTGGACTCTACTGCACGCATTGGTGACTCGGGCTCGATTGGCATGGCTTACACCGATGGTTCTACTTATAAGGAAATTAGAGCTGTTACGATTGATGACCTCATTCAAAGTGTTAATGAAAAAACGAAGTACTTAATTGATTTCATTAACACTAACACGGAAATCTTCTTCCAAAAGTACGGCTTTTTACCCAGCACTTTACTGTTTTACACTAAGAGTAAACAGTTGCTTAACAACTTTCAACTCAACCAAAACCTGTTGAGTAACCACTTCAAAACTGTAACCATCTTTAAAAACGAAGTTCAGTTTGTGAGTCGCAAATACTTATTGAACTGTCAAGCAATTGCGCTTTCTTGA", "aa": "MYNLKNIYASITLYPHEINLVVSDNNNQFHVLYENSIANDELYTHAGITNKAKFKVVLNQLINNANDYLGFKLEKVIVVLAELVDDLEINRFKTDVFFTGYDFNHQDMLSSEKNRFLLKNRHINPNEVLDLVALNYRDLTTDKISKNFKYNCSYRANVIYYTTKNSLVKELKPFLKRNIIVKIDKIVTHHMVLAHSIKQINKNNLFVYLGEHTTDLMLFMDNALVDLVSEPFGRVNFLESDNDSENKALLEFLVDSTARIGDSGSIGMAYTDGSTYKEIRAVTIDDLIQSVNEKTKYLIDFINTNTEIFFQKYGFLPSTLLFYTKSKQLLNNFQLNQNLLSNHFKTVTIFKNEVQFVSRKYLLNCQAIALS", "aa_hexdigest": "9f1015fbc587b75bdfd2ba3093a21304", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75465", "query_cov": 1.0026954177897573, "subject_cov": 0.8857142857142857, "identity": 0.995, "score": 720.0, "evalue": 3.65e-261, "valid": true, "product": "MPN316 family protein", "uniref50_id": "UniRef50_P47465" }, "pscc": { "uniref50_id": "UniRef50_P47465", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47465" ], "product": "Uncharacterized protein MG223" }, "genes": [], "id": "APKECPOBEN_696", "locus": "GCA002090215_00669" }, { "type": "cds", "sequence": "contig_1", "start": 376222, "stop": 377013, "strand": "+", "frame": 1, "gene": "ftsZ", "product": "cell division protein FtsZ", "db_xrefs": [ "COG:COG0206", "COG:D", "GO:0000910", "GO:0000917", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0032153", "GO:0043093", "GO:0051258", "KEGG:K03531", "SO:0001217", "UniRef:UniRef50_P47466", "UniRef:UniRef90_P75464" ], "nt": "ATGTTGCGCATGCACCCCGAATTGCAAACGGCTAACGTCCAGTTCTTTGCTTTAAACACTGATTTACAGCACTTAAAAACAAAGCGCTATGTCCAAAATAAGGCTGTGATTCAGTTTGAAGAATCCAAGGGCTTAGGTGTTGGTGGTGATCCCCAAAAAGGTGCCGTTTTAGCACACCATTTTTTAGAGCAATTTCACAAACTTAGTGACAGCTTTGATTTCTGTATTTTAGTCGCTGGTTTTGGTAAGGGGACTGGTACTGGTGCTACCCCGGTTTTTAGTAAATTTCTTAGCAACAAAGGGGTGTTAAATCTCTCGATTGTCAGTTACCCCGCAATGTGTGAAGGGTTAAAGGCACGCGAAAAAGCAGCTAAAGGTTTAGAACGCTTAAACCAAGCGACTGACAGTTTTATGCTGTTCCGCAATGATCGCTGTACTGATGGCATTTACCAACTAGCAAACGTTGCCATTGTCAAAACAATTAAAAACATTATTGAACTAATTAATCTACCACTACAGCAAAACATTGATTTTGAAGACATTCGTTCTTTCTTTAAAAAACCAGCACAACGTTTAGAGAATGAAGCTAACTTGTTTCGCGTAACTAATACTTTTACTTTTAGCTTTGACGCGCACAATACAATTGAGCACTTTAGCCACAAATTGAAAAACTTTGAGTATGAAGGTTTTTTTGACCATAAGGTAGAAGGGGCGCAAAAGGTAATTTTAAAGGTGTTAGTCAACCAAGGTTTATATCCGCTTGATTTAACCCAAATTCAAGAGATTATTTGA", "aa": "MLRMHPELQTANVQFFALNTDLQHLKTKRYVQNKAVIQFEESKGLGVGGDPQKGAVLAHHFLEQFHKLSDSFDFCILVAGFGKGTGTGATPVFSKFLSNKGVLNLSIVSYPAMCEGLKAREKAAKGLERLNQATDSFMLFRNDRCTDGIYQLANVAIVKTIKNIIELINLPLQQNIDFEDIRSFFKKPAQRLENEANLFRVTNTFTFSFDAHNTIEHFSHKLKNFEYEGFFDHKVEGAQKVILKVLVNQGLYPLDLTQIQEII", "aa_hexdigest": "bbc85d5f84792e73cad421505bb2db29", "start_type": "ATG", "rbs_motif": "AACAA", "genes": [ "ftsZ" ], "truncated": "both", "pseudogene": { "start": 376117, "stop": 377259, "observations": { "mutation": [ 376123, 377011 ], "stop": [ 376123, 377011 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75464", "query_cov": 1.0, "subject_cov": 0.6921052631578948, "identity": 1.0, "score": 524.0, "evalue": 2.57e-186, "gene_start": 1, "gene_end": 263, "reference_start": 36, "reference_end": 298, "reference_sequence": "MDWIQTAGAGTQLPENNIKIAVFGIGGAGNNIIDDMLRMHPELQTANVQFFALNTDLQHLKTKRYVQNKAVIQFEESKGLGVGGDPQKGAVLAHHFLEQFHKLSDSFDFCILVAGFGKGTGTGATPVFSKFLSNKGVLNLSIVSYPAMCEGLKAREKAAKGLERLNQATDSFMLFRNDRCTDGIYQLANVAIVKTIKNIIELINLPLQQNIDFEDIRSFFKKPAQRLENEANLFRVTNTFTFSFDAHNTIEHFSHKLKNFEYEGFFDHKVEGAQKVILKVLVNQGLYPLDLTQIQEIIWAKIDNHNLEVQLGVDFTDANPSVQLFFLMEKKQAVSSDFIQKPAFISVKEVNQKPAKPFQVLNDLKELGLKYVKQQTGFNY" }, "paralog": false, "identity": 0.9947368421052631, "score": 750.0, "evalue": 1.07e-277, "description": "Internal stop codon at 376123, 377011. Nonsense mutation around 376123, 377011.", "psc": { "uniref90_id": "UniRef90_P75464", "gene": "ftsZ", "product": "cell division protein FtsZ", "uniref50_id": "UniRef50_P47466", "cog_id": "COG0206", "cog_category": "D", "kegg_orthology_id": "K03531", "go_ids": [ "GO:0000910", "GO:0000917", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0032153", "GO:0043093", "GO:0051258" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75464", "UniRef:UniRef50_P47466", "COG:COG0206", "COG:D", "KEGG:K03531", "GO:0000910", "GO:0000917", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0032153", "GO:0043093", "GO:0051258" ] }, "pscc": { "uniref50_id": "UniRef50_P47466", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47466" ], "product": "Cell division protein FtsZ" } }, "id": "APKECPOBEN_697", "locus": "GCA002090215_00670" }, { "type": "cds", "sequence": "contig_1", "start": 377080, "stop": 377259, "strand": "+", "frame": 1, "gene": "ftsZ", "product": "Cell division protein FtsZ", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W8D0", "UniRef:UniRef90_A0AB38W8D0" ], "nt": "GTGCAACTGTTTTTCTTAATGGAGAAAAAGCAAGCAGTGTCCTCTGACTTTATTCAAAAACCAGCATTTATCTCAGTTAAAGAAGTAAATCAGAAACCGGCCAAACCGTTCCAAGTTTTGAATGATTTAAAGGAGCTGGGACTGAAATACGTCAAACAGCAAACCGGATTTAATTATTAG", "aa": "MQLFFLMEKKQAVSSDFIQKPAFISVKEVNQKPAKPFQVLNDLKELGLKYVKQQTGFNY", "aa_hexdigest": "16a3b4888b1450bbcb56766c25c74535", "start_type": "GTG", "rbs_motif": null, "genes": [ "ftsZ" ], "truncated": "5-prime", "pseudogene": { "start": 376885, "stop": 377259, "observations": { "mutation": [ 377011 ], "stop": [ 377011 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB38W8D0", "query_cov": 0.9830508474576272, "subject_cov": 0.46774193548387094, "identity": 1.0, "score": 115.0, "evalue": 7.25e-32, "gene_start": 2, "gene_end": 59, "reference_start": 67, "reference_end": 124, "reference_sequence": "MKNFEYEGFFDHKVEGAQKVILKVLVNQGLYPLDLTQIQEIIWAKIDNHNLEVQLGVDFTDANPSVQLFFLMEKKQAVSSDFIQKPAFISVKEVNQKPAKPFQVLNDLKELGLKYVKQQTGFNY" }, "paralog": false, "identity": 0.9838709677419355, "score": 243.0, "evalue": 6.28e-85, "description": "Internal stop codon at 377011. Nonsense mutation around 377011.", "psc": { "uniref90_id": "UniRef90_A0AB38W8D0", "product": "Cell division protein FtsZ", "uniref50_id": "UniRef50_A0AB38W8D0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB38W8D0", "UniRef:UniRef50_A0AB38W8D0" ] }, "pscc": { "uniref50_id": "UniRef50_A0AB38W8D0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W8D0" ], "product": "Cell division protein FtsZ" } }, "id": "APKECPOBEN_698", "locus": "GCA002090215_00671" }, { "type": "cds", "sequence": "contig_1", "start": 377302, "stop": 377424, "strand": "+", "frame": 1, "gene": null, "product": "amino acid permease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0027DFE8A8", "UniRef:UniRef90_UPI0027DFE8A8" ], "nt": "ATGGCTGTTGCAAGTAAGAAGTTCACTGAACGTCAATTTTTATTTTTTGTTGTCAACTATATTGCTGGCTTTGGTTTTATTTCCACTACCCTATCACTGTTTGAGCTAGGACCACTTTCATGA", "aa": "MAVASKKFTERQFLFFVVNYIAGFGFISTTLSLFELGPLS", "aa_hexdigest": "2b624d0fe95090bcc8ee37d664b4cfb3", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 377302, "stop": 377568, "observations": { "mutation": [ 377422, 377524 ], "stop": [ 377422, 377524 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0027DFE8A8", "query_cov": 1.0, "subject_cov": 0.45454545454545453, "identity": 0.975, "score": 77.8, "evalue": 6.85e-18, "gene_start": 1, "gene_end": 40, "reference_start": 1, "reference_end": 40, "reference_sequence": "MAVASKKFTERQFLFFVVNYIAGFGFISTALSLFELGPLSWVIFFIVSLISLIVTLAFARVSAIDKNNYGGAYLWAKKAFNSERYSHR" }, "paralog": false, "identity": 0.9545454545454546, "score": 161.0, "evalue": 2.14e-53, "description": "Internal stop codon at 377422, 377524. Nonsense mutation around 377422, 377524.", "psc": { "uniref90_id": "UniRef90_UPI0027DFE8A8", "product": "amino acid permease", "uniref50_id": "UniRef50_UPI0027DFE8A8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0027DFE8A8", "UniRef:UniRef50_UPI0027DFE8A8" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0027DFE8A8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0027DFE8A8" ], "product": "amino acid permease" } }, "id": "APKECPOBEN_699", "locus": "GCA002090215_00672" }, { "type": "cds", "sequence": "contig_1", "start": 377806, "stop": 378777, "strand": "+", "frame": 1, "gene": null, "product": "APC family permease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001FB1D5B2", "UniRef:UniRef90_UPI001FB1D5B2" ], "nt": "GTGCTTTTGAGCACCTTAATAGTAGCACTTATAGTTATCGGCAAAAATGGTAATGGTTATGCCATTAACAACGACATAAAACAAGGCCCTTTTAAAGAACGTGAAATTACCTTGGGTGCAGTTGCTACTGTCTTTATTACCTTCTTTTATTCCTACAGTGGTGCGGAGGACATTTCAACAATGACACCGGATGTGAAAACCACTAACTTCCGCAAGATTTTAATCATCTCTTTTATTGCGGTATTCTTGTTTTACTTTGTGGGCATTTTGATCTTTAATGGTTTATTGAATGTTTCACAAAGCGATGATGATAAGGGGAAAGATGGAAGAATAGCTAGTGTAGCTGATATTTTTAGATTAGCTGGCGGTTTAGGGGCACTCATCTTTTACGGTGTGGGTGCTTTATTCAATAACGTATCTACCAGACTTTCGACCATTATTGCTAACTCACGCAAGATCTTACCGTTAGCACAAGATAACTATTTACCCACTACCTTTAGCAAAGCTAACAGTAAGGGCGAGTTCCAAAATGCAATTTGGTTTACCTTTGGTACTACCATCATTACGATGACGGTACTGTTCTTTGTTCCGCTAATAACCAGCAACTTTGACTTCGACAAATCAACCGAGTATGCCGCCTCGATAGGTTCGGCCGCTACACTGGTACAATATATCCTTGTATTTTTTATAATTTTTAGGCTTATTTATAAAAAAGAGCAACTTTATCAAAAAAACTGGACACGCGAGTTAGAACAAATACTGTTTGGGATTGGTGTCTTTATTATTCTTCTCATGTTGCTCTTTTATCTCTTTCCAGTAATTGATGGAAAAACTAAATGGCAATTGAAAAATACCCTCACTTTAGTGGTGTATGGGGTGTTGATTTTAATTGGATTTGCCCTCTTTATGACCCAAGAATATAAGCGCAATAAACAAAAGGAAAGCTATGTCACACCAAGAGCAGTTACATAA", "aa": "MLLSTLIVALIVIGKNGNGYAINNDIKQGPFKEREITLGAVATVFITFFYSYSGAEDISTMTPDVKTTNFRKILIISFIAVFLFYFVGILIFNGLLNVSQSDDDKGKDGRIASVADIFRLAGGLGALIFYGVGALFNNVSTRLSTIIANSRKILPLAQDNYLPTTFSKANSKGEFQNAIWFTFGTTIITMTVLFFVPLITSNFDFDKSTEYAASIGSAATLVQYILVFFIIFRLIYKKEQLYQKNWTRELEQILFGIGVFIILLMLLFYLFPVIDGKTKWQLKNTLTLVVYGVLILIGFALFMTQEYKRNKQKESYVTPRAVT", "aa_hexdigest": "eb16bf6966326977e9ed64777290fb65", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001FB1D5B2", "query_cov": 1.0, "subject_cov": 0.8801089918256131, "identity": 0.9940000000000001, "score": 613.0, "evalue": 8.3e-221, "valid": true, "product": "APC family permease", "uniref50_id": "UniRef50_UPI001FB1D5B2" }, "pscc": { "uniref50_id": "UniRef50_UPI001FB1D5B2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001FB1D5B2" ], "product": "APC family permease" }, "genes": [], "id": "APKECPOBEN_700", "locus": "GCA002090215_00673" }, { "type": "cds", "sequence": "contig_1", "start": 378752, "stop": 379063, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002D8CD42" ], "nt": "ATGTCACACCAAGAGCAGTTACATAAACCTAACCGGCAACAGTTTAGCGAAAAGCAATTTATTGCTTTTGCTTTTAACTATGTTGCTGGGTTCGGCTTTATTTCCGTGGTGCTAACCATGTTTAAGCTTGGCCCGTTTTCTTACTTGATCCTAGGGCTAGCAGCTTTAGGTATTTTGGGGGTAATGTTGTCCTTTTCGCGCCTTTCAATTATTTGTGGTAGCAAGGCTTACGGTGGTAGTTATTTAATAGCCAAAAAAGCACTTGGTGTGAAAACAATCACGGCGCGTTTCTTTACTTTTTTGAGTGGTTGA", "aa": "MSHQEQLHKPNRQQFSEKQFIAFAFNYVAGFGFISVVLTMFKLGPFSYLILGLAALGILGVMLSFSRLSIICGSKAYGGSYLIAKKALGVKTITARFFTFLSG", "aa_hexdigest": "49c781556a9fa06b0c0ec9b28a24047e", "start_type": "ATG", "rbs_motif": "AATAA", "psc": { "uniref90_id": "UniRef90_UPI0002D8CD42", "query_cov": 0.9223300970873787, "subject_cov": 0.8878504672897196, "identity": 0.705, "score": 133.0, "evalue": 1.86e-38, "valid": false, "uniref50_id": "UniRef50_UPI0002D8CD42" }, "pscc": { "uniref50_id": "UniRef50_UPI0002D8CD42", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002D8CD42" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11309.294599999981, "isoelectric_point": 10.08564357757568 }, "id": "APKECPOBEN_701", "locus": "GCA002090215_00674" }, { "type": "cds", "sequence": "contig_1", "start": 379100, "stop": 379276, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCCAGCGGTGCTAGTTCTCTCGTTTGCTGATATTAAAGCAGTGAAGGATAACAATGGTGCTTTAATTGGCTTACTGGTCGGGGGCTTTGTTTTATTTGGGGCATTAAACTTCATCTCCCTGTTTGGCCTTAAAATGAATAAGAACGCCATTCTCTTCTTTGCCATAGTGAAGTGA", "aa": "MPAVLVLSFADIKAVKDNNGALIGLLVGGFVLFGALNFISLFGLKMNKNAILFFAIVK", "aa_hexdigest": "ef8547207aa41428e2ef7e35cd19c12f", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6155.446099999997, "isoelectric_point": 9.82448215484619 }, "id": "APKECPOBEN_702", "locus": "GCA002090215_00675" }, { "type": "cds", "sequence": "contig_1", "start": 379301, "stop": 379831, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00324.27" ], "nt": "TTGGGAATCTACCTCATCGGTACGAACCACGGCCATGGTTTTGTCGAAAACAACACTTTAGGTGAACACATTGAAGATCTCAGTTTCCTTAAAGTTATTTCTACTACTGTGGGGATGTTGGTGGCCTTTGCTGGTACCGAAGATCTTACTGCGATTACCCCGGATGTGAAGTCAAAAAACATCCGTAAGTGTTTCTTATTGATGTTTGGTGCAGTTACCCTGCTTTATCTAATCGGTTTTGTCATTATTAGTGGGATTTCTGGTTTAAATGGTTATGGCCTTGATGGTAAAGAAAAGAATGAAAAAGCGATTAACACCTTTGGTTCGATTTACTTTCAAGCAGGCGGTAAGTATTTAGGCATCCCACTGTTGGTCATCTTTGGTCTGGGTTTTTTACTCAATTCCTTGGCATCACGATTAGGCATGACCATTACGACAGCGCGCAAATACGTTGCGTTAGCCCAAGATGGTTTCTTGCCGTCCTTTATTAACGAACAAAACAAGCACCACGAGTACCATAAGGCAGTGTGA", "aa": "MGIYLIGTNHGHGFVENNTLGEHIEDLSFLKVISTTVGMLVAFAGTEDLTAITPDVKSKNIRKCFLLMFGAVTLLYLIGFVIISGISGLNGYGLDGKEKNEKAINTFGSIYFQAGGKYLGIPLLVIFGLGFLLNSLASRLGMTITTARKYVALAQDGFLPSFINEQNKHHEYHKAV", "aa_hexdigest": "d078806c438e1273d34bd674bb283a15", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00324.27", "name": "AA_permease", "length": 169, "aa_cov": 0.9431818181818182, "hmm_cov": 0.9349112426035503, "evalue": 1.6511390091096488e-13, "score": 45.527122497558594, "start": 11, "stop": 176 } ], "seq_stats": { "molecular_weight": 19119.021000000008, "isoelectric_point": 7.866964149475098 }, "id": "APKECPOBEN_703", "locus": "GCA002090215_00676" }, { "type": "cds", "sequence": "contig_1", "start": 379844, "stop": 380059, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACCTTAGCAGTGATGGTGCTGATGATCATTGTCCCCTTTTTACCTAATGATGAAAACCCCGGTAAACAGCTCGTTATGTTCGATGCCATTAGTGTTTTAGTTGAAGTGGCGATTGAACTAGCAGTGTTAATTTCCTTAATCCAGTACTTTATTACCTACATCTTCTTCTTTATGATTCTGGCCAAAAAAGAGGGTAGTGCCAGTGTTTCGTGA", "aa": "MTLAVMVLMIIVPFLPNDENPGKQLVMFDAISVLVEVAIELAVLISLIQYFITYIFFFMILAKKEGSASVS", "aa_hexdigest": "19ea4c89d06288c76d5d0ab287d188c5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7897.521499999995, "isoelectric_point": 4.3588346481323255 }, "id": "APKECPOBEN_704", "locus": "GCA002090215_00677" }, { "type": "cds", "sequence": "contig_1", "start": 380635, "stop": 380913, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00303.24" ], "nt": "ATGAGCTTTAAAATAACCGTTTTAAGTTATTGCAAAAACTCTTTTAAAAAAGTTATTCGCTTATTTTGCAAGCTTAAAAAAAGATTTTTATACAATGCTACACCATCGAAGTTTAGGAGAGTTATGCAGCAGTATCTAGATTTAGCCCGTTATGTTTTAGAGCACGGAAAATACCGTCCAAATCGCACTGATACTGCTGGCATTGGTGTCTTTGGTTACCAGATGCACTTTGATATTTCAAAACACTTTCCGCTCTTGACTACGAAGAAGGTGCACTGA", "aa": "MSFKITVLSYCKNSFKKVIRLFCKLKKRFLYNATPSKFRRVMQQYLDLARYVLEHGKYRPNRTDTAGIGVFGYQMHFDISKHFPLLTTKKVH", "aa_hexdigest": "8badef1771ba78bade1c445ee768f036", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00303.24", "name": "Thymidylat_synt", "length": 51, "aa_cov": 0.5434782608695652, "hmm_cov": 1.0, "evalue": 3.0322906459424597e-15, "score": 51.4938850402832, "start": 43, "stop": 92 } ], "seq_stats": { "molecular_weight": 10995.94419999999, "isoelectric_point": 10.29677753448486 }, "id": "APKECPOBEN_705", "locus": "GCA002090215_00678" }, { "type": "cds", "sequence": "contig_1", "start": 381250, "stop": 381621, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00303.24" ], "nt": "ATGTTGCTCCCGCCTTGTCATTCACTGTACCAGTTTTACGTACAGGACGGGCAGTTGAGCTGCCAGTTGTACCAGCGCAGTGGTGATATTTTTCTAGGTATCCCCTTCAACATTGCGTCCTACTCTCTTCTGGTGTACTTAGTTGCTAAGGAAACTAACCTAAAACCAGGGAGCTTTGTCCACACCATTGGCGATGCCCACATTTACGAAAATCACTTGGAACAAATTAAGTTGCAATTAACACGTCAACCCAAACCATTGCCCAAGGTAGTTTTAAAGAGTGATAAATCAATTTTTGATTATCAATTCGATGATATTGAACTGGTGGATTACGACCACCACCCTACAATTAAAGGAGAGGTAGCAGTTTAA", "aa": "MLLPPCHSLYQFYVQDGQLSCQLYQRSGDIFLGIPFNIASYSLLVYLVAKETNLKPGSFVHTIGDAHIYENHLEQIKLQLTRQPKPLPKVVLKSDKSIFDYQFDDIELVDYDHHPTIKGEVAV", "aa_hexdigest": "299a13719ecd353eabe1e055efd5df75", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00260828A0", "query_cov": 1.008130081300813, "subject_cov": 0.8104575163398693, "identity": 0.637, "score": 174.0, "evalue": 8.86e-54, "valid": false, "gene": "thyA", "product": "thymidylate synthase", "ec_ids": [ "2.1.1.45" ], "uniref50_id": "UniRef50_A0A059X7H5", "kegg_orthology_id": "K00560", "go_ids": [ "GO:0004799", "GO:0006231" ] }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00303.24", "name": "Thymidylat_synt", "length": 126, "aa_cov": 1.0, "hmm_cov": 1.0, "evalue": 2.2803634537972213e-56, "score": 186.31985473632812, "start": 1, "stop": 123 } ], "seq_stats": { "molecular_weight": 14107.032299999983, "isoelectric_point": 5.7831628799438475 }, "id": "APKECPOBEN_706", "locus": "GCA002090215_00679" }, { "type": "cds", "sequence": "contig_1", "start": 381746, "stop": 382105, "strand": "+", "frame": 2, "gene": "folA", "product": "Dihydrofolate reductase", "db_xrefs": [ "COG:COG0262", "COG:H", "EC:1.5.1.3", "GO:0004146", "GO:0005829", "GO:0006730", "GO:0046452", "GO:0046654", "GO:0046655", "GO:0050661", "KEGG:K00287", "SO:0001217", "UniRef:UniRef50_P78028", "UniRef:UniRef90_P78028" ], "nt": "ATGGGCTCGGCTACCTATCTTTCCTTACCGCCGGTGTTTAGTGAGCGTAACGTTTATATCCTCACCCGTAACCTCAACTTTAACCCGCCTGATAAGGGCTGTTTGACTAAAGTAATCCATGAATATGAAAACTTTATCCAACCTTATTTGCACCATCCCGATAAACACCTCTACATCTGTGGTGGGGCACAAGTTTATGAACAGCTCATTCCCCGGTGTGATGCGTTAATTGTCTCTACCATTTTCGGTAAATACACCGGGGACAAATACCTCAAAGTGGATTTTAGTCCCTTTGAATTGACCAAAGAGATCAGTTTTGCTGAGTTTAAGGTCGCTTACTATCACAAAATTGCACGATAA", "aa": "MGSATYLSLPPVFSERNVYILTRNLNFNPPDKGCLTKVIHEYENFIQPYLHHPDKHLYICGGAQVYEQLIPRCDALIVSTIFGKYTGDKYLKVDFSPFELTKEISFAEFKVAYYHKIAR", "aa_hexdigest": "06c5e173ec0cec6d25ce0aa36513a4ef", "start_type": "ATG", "rbs_motif": null, "genes": [ "folA" ], "truncated": "5-prime", "pseudogene": { "start": 381623, "stop": 382105, "observations": { "mutation": [ 381638, 381686 ], "stop": [ 381638, 381686 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P78028", "query_cov": 1.0, "subject_cov": 0.74375, "identity": 1.0, "score": 251.0, "evalue": 3.2e-84, "gene_start": 1, "gene_end": 119, "reference_start": 42, "reference_end": 160, "reference_sequence": "MVKAIWAMDQNGLIGNGNSLPWRIKAELQHFRQTTLHQDVLMGSATYLSLPPVFSERNVYILTRNLNFNPPDKGCLTKVIHEYENFIQPYLHHPDKHLYICGGAQVYEQLIPRCDALIVSTIFGKYTGDKYLKVDFSPFELTKEISFAEFKVAYYHKIAR" }, "paralog": false, "identity": 0.9875, "score": 325.0, "evalue": 1.12e-115, "description": "Internal stop codon at 381638, 381686. Nonsense mutation around 381638, 381686.", "psc": { "uniref90_id": "UniRef90_P78028", "gene": "folA", "product": "Dihydrofolate reductase", "ec_ids": [ "1.5.1.3" ], "uniref50_id": "UniRef50_P78028", "cog_id": "COG0262", "cog_category": "H", "kegg_orthology_id": "K00287", "go_ids": [ "GO:0005829", "GO:0004146", "GO:0050661", "GO:0046452", "GO:0046655", "GO:0006730", "GO:0046654" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P78028", "EC:1.5.1.3", "UniRef:UniRef50_P78028", "COG:COG0262", "COG:H", "KEGG:K00287", "GO:0005829", "GO:0004146", "GO:0050661", "GO:0046452", "GO:0046655", "GO:0006730", "GO:0046654" ] }, "pscc": { "uniref50_id": "UniRef50_P78028", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P78028" ], "product": "Dihydrofolate reductase" } }, "id": "APKECPOBEN_707", "locus": "GCA002090215_00680" }, { "type": "cds", "sequence": "contig_1", "start": 382112, "stop": 382204, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTAACACAAAAAAATACTTTTTAGAATCAGTTTCTCCCTTAGAATATGCACAAAAAAAGTTTCAGGGCAACTTGCGCTCGGTTAACTGA", "aa": "MANTKKYFLESVSPLEYAQKKFQGNLRSVN", "aa_hexdigest": "fa66f01242337ad0ed68da612541c86a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3491.9673000000003, "isoelectric_point": 9.69799518585205 }, "id": "APKECPOBEN_708", "locus": "GCA002090215_00681" }, { "type": "cds", "sequence": "contig_1", "start": 382460, "stop": 383122, "strand": "+", "frame": 2, "gene": null, "product": "ribonucleoside-diphosphate reductase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A376CKW9" ], "nt": "ATGGTGGGGGTACACGCGCGCTCTTATGGCACCATCTTTTCCACTCTGTGTACTTCAGAACAAATTACTGAAGCACATGAATGGGTGGTGAAAACCGAAAGCTTGCAAAAGCGCGCTAAGGCTTTAATTCCCTATTACACCGGGAAAGACCCATTAAAGTCTAAGGTAGCAGCCGCTTTAATGCCCGGGTTCTTACTGTATGGTGGGTTTTATTTACCGTTTTACCTCTCCTCGCGTAAACAGCTACCCAACACTTCGGACATTATTCGCTTAATTTTGCGCGATAAGGTAATCCATAACTATTACAGTGGGTATAAGTTCCAGCGTAAAGTGGAAAAGATGTCCAAGGAAAAACAGGCTGAGATGAAACGGTTTGTCTTTGATTTAATGTACGAACTGATTGAGTTGGAAAAGGCATACCTCAAGGAACTGTATGAAGGGTTTGGGATTGTAGAAGATGCCATTAAATTCAGTATTTATAACGCTGGTAAGTTTTTGCAAAACTTAGGGTATGACTCACCGTTTACAGAAGAAGAAACGCGGATTAAACCAGAAATTTTTGCCCAATTGTCAGCCAGAGCCGATGAAAACCACGATTTCTTTTCTGGTAATGGTTCGTCATATGTGATGGGAATTAGTGAAGAGACCGAAGATAAGGATTGA", "aa": "MVGVHARSYGTIFSTLCTSEQITEAHEWVVKTESLQKRAKALIPYYTGKDPLKSKVAAALMPGFLLYGGFYLPFYLSSRKQLPNTSDIIRLILRDKVIHNYYSGYKFQRKVEKMSKEKQAEMKRFVFDLMYELIELEKAYLKELYEGFGIVEDAIKFSIYNAGKFLQNLGYDSPFTEEETRIKPEIFAQLSARADENHDFFSGNGSSYVMGISEETEDKD", "aa_hexdigest": "4567b7e2d05af6d18aceebc0821d9282", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A9E3ENY0", "query_cov": 1.0, "subject_cov": 0.9565217391304348, "identity": 0.505, "score": 227.0, "evalue": 4.71e-72, "valid": false, "gene": "nrdF", "product": "class 1b ribonucleoside-diphosphate reductase subunit beta", "ec_ids": [ "1.17.4.1" ], "uniref50_id": "UniRef50_A0A376CKW9", "go_ids": [ "GO:0004748", "GO:0005971", "GO:0009263" ] }, "pscc": { "uniref50_id": "UniRef50_A0A376CKW9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A376CKW9" ], "product": "ribonucleoside-diphosphate reductase" }, "genes": [], "id": "APKECPOBEN_709", "locus": "GCA002090215_00682" }, { "type": "cds", "sequence": "contig_1", "start": 383134, "stop": 383595, "strand": "+", "frame": 1, "gene": "nrdI", "product": "class Ib ribonucleoside-diphosphate reductase assembly flavoprotein NrdI", "db_xrefs": [ "COG:COG1780", "COG:F", "GO:0009263", "GO:0010181", "GO:0036211", "KEGG:K03647", "RefSeq:WP_010874679.1", "SO:0001217", "UniParc:UPI00001304DF", "UniRef:UniRef100_P75460", "UniRef:UniRef50_P47472", "UniRef:UniRef90_P75460" ], "nt": "ATGCATAAAGATATCAAAATAGTCGACGCTAGTGCGATCGTTAAACCAACTGGAACACCGTATGTAGTGTATTTTTCCTCCATTTCTAATAACACCCACCGTTTTATTGAAAAATTGGAGTTTGAACACACCCGCATTCCGGTTAATTTAGATGAACAGATTGAAGTAAACCAGGAGTATGTGCTCTTTTGCCCCACTTACAGTGGTGGTGGGGAATACACATCGGGGGCGGTACCCAAACAGGTAATCCACTTTTTAAACAACAAGCACAACCGTGATTTGTGTCGTGGCGTAATTTCGTCCGGGAACACTAATTTCGGCAATACTTTTGCCATTGCCGGGCCGATTTTGTCGAAAAAGTTAAACGTACCACTTTTATACCAGTTTGAACTTTTAGGCACGAAAAACGATGTAGAACAAGTTCAAACCATTATTACCAATTTCTTTGGTAAAGCCAAATAA", "aa": "MHKDIKIVDASAIVKPTGTPYVVYFSSISNNTHRFIEKLEFEHTRIPVNLDEQIEVNQEYVLFCPTYSGGGEYTSGAVPKQVIHFLNNKHNRDLCRGVISSGNTNFGNTFAIAGPILSKKLNVPLLYQFELLGTKNDVEQVQTIITNFFGKAK", "aa_hexdigest": "04747b209f09a77cbd7c237cd164a751", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001304DF", "ncbi_nrp_id": "WP_010874679.1", "uniref100_id": "UniRef100_P75460", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001304DF", "RefSeq:WP_010874679.1", "UniRef:UniRef100_P75460" ] }, "ips": { "uniref100_id": "UniRef100_P75460", "uniref90_id": "UniRef90_P75460" }, "psc": { "uniref90_id": "UniRef90_P75460", "gene": "nrdI", "product": "class Ib ribonucleoside-diphosphate reductase assembly flavoprotein NrdI", "uniref50_id": "UniRef50_P47472", "cog_id": "COG1780", "cog_category": "F", "kegg_orthology_id": "K03647", "go_ids": [ "GO:0009263", "GO:0010181", "GO:0036211" ] }, "pscc": { "uniref50_id": "UniRef50_P47472", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47472" ], "product": "Protein NrdI" }, "genes": [ "nrdI" ], "id": "APKECPOBEN_710", "locus": "GCA002090215_00683" }, { "type": "cds", "sequence": "contig_1", "start": 383619, "stop": 385247, "strand": "+", "frame": 3, "gene": null, "product": "class 1b ribonucleoside-diphosphate reductase subunit alpha", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021AFF10F" ], "nt": "ATGTCAGTTAAAGAAAAGATTCCTGCTTTCAACACCCAAGAAGACTTAGAAAGCTATATTTCCTTAAATGCCTACACTAAGGTGTACGGTGATTTTAAGATGGATTTACACGCTGTGGAGGCTTACATCCAAGAGCATGTCAAACCCAAAACAAAGGTGTTTCACTCCACCAAGGAACGCTTAGACTTTCTAGTAAAGAATGATTATTACGATGAGAACATCATCAACATGTACAGCTTTGAGCAGTTTGAGGAAATTACACGTAAGGCTTACGCTTACCGTTTTCGTTATGCTAACTTTATGGGGGCATTTAAGTTTTACAACGCTTATGCACTGAAAACCTTTGATGGGAAGTGGTATTTAGAAAACTATGAAGACCGCGTTGTCATGAACGTGCTCTTTTTAGCCAATGGTAACTATAACAAGGCTTTAAAGTTACTCAAACAAATTATTACCAACCGCTTTCAACCAGCTACCCCAACTTTTTTAAATGCGGGACGAAAAAAACGTGGTGAGTTTGTGTCGTGTTACTTACTGCGCATAGAAGACAACATGGAATCAATTGGTCGTGCCATTACCACTACCCTACAGCTCTCCAAGCGTGATGGTGGGGTGGCGTTATTGCTCACTAACATCCGCGAATCGGGTGCGCCAATTAAAAAAATAGAAAACCAGTCATCAGGCATTATTCCAATTATGAAGCTGTTGGAAGACTCTTTTTCTTACGCCAACCAATTAGGGCAAAGACAGGGAGCAGGAGCAGTGTATCTCCATGCCCACCACCCCGATGTCATGCAGTTTTTAGACACAAAGCGCGAAAACGCTGATGAAAAGATCCGGATTAAGTCGCTGTCTTTGGGTCTAGTTATTCCCGACATTACCTTTACTTTAGCTAAAAACAATGAGGAGATGGCCTTGTTTAGTCCGTATGATGTGTATGAGGAGTATGGCAAACCCTTGTCAGACATTTCAGTCACGGAAATGTATTACGAACTGTTAGCGAACCAACGCATTAAAAAGACCTTTATTAATGCGCGCAAGTTCTTCCAAACAGTGGCGGAACTGCACTTTGAAAGCGGTTATCCTTACATCTTATTTGACGATACGGTTAACCGGCGCAACGCGCACCCCAACCGCATTGTCATGTCTAACCTCTGTAGTGAAATTGTCCAACCCTCCACTCCATCGGAGTTCCACCACGATTTGGCTTTTAAAAAGGTTGGTAATGACATTAGCTGTAACTTGGGTAGCTTAAACATTGCCAAAGCAATGGAAAGCGGACCGGAGTTCTCGGAGTTGGTTAAACTAGCGATCGAATCACTTGATTTAGTTTCAAGGGTAAGCAATTTAGAAACGGCTCCATCGATCCAAAAGGGTAATTCCGAAAATCATGCTTTGGGATTAGGGGCAATGAACTTGCACGGTTTTTTAGCAACTAACCAAATTTATTACAACTCCCCTGAAGCAATCGACTTTACTAACATCTTTTTCTATACGGTAGCTTACCATGCCTTTAAAGCATCCAGTGAGCTAGCTTTAGAGAAGGGTAAGTTTAAAAACTTTGAAAACACCAAGTTTGCTGATGGGAGCTACTTTGATAAATATATCAAAGTAGAACCAGACTTTTGA", "aa": "MSVKEKIPAFNTQEDLESYISLNAYTKVYGDFKMDLHAVEAYIQEHVKPKTKVFHSTKERLDFLVKNDYYDENIINMYSFEQFEEITRKAYAYRFRYANFMGAFKFYNAYALKTFDGKWYLENYEDRVVMNVLFLANGNYNKALKLLKQIITNRFQPATPTFLNAGRKKRGEFVSCYLLRIEDNMESIGRAITTTLQLSKRDGGVALLLTNIRESGAPIKKIENQSSGIIPIMKLLEDSFSYANQLGQRQGAGAVYLHAHHPDVMQFLDTKRENADEKIRIKSLSLGLVIPDITFTLAKNNEEMALFSPYDVYEEYGKPLSDISVTEMYYELLANQRIKKTFINARKFFQTVAELHFESGYPYILFDDTVNRRNAHPNRIVMSNLCSEIVQPSTPSEFHHDLAFKKVGNDISCNLGSLNIAKAMESGPEFSELVKLAIESLDLVSRVSNLETAPSIQKGNSENHALGLGAMNLHGFLATNQIYYNSPEAIDFTNIFFYTVAYHAFKASSELALEKGKFKNFENTKFADGSYFDKYIKVEPDF", "aa_hexdigest": "cd26c88bade2a43f72ffb6d4e5e9a016", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001C049A88", "query_cov": 0.9022140221402214, "subject_cov": 0.9683168316831683, "identity": 0.6809999999999999, "score": 682.0, "evalue": 5.51e-242, "valid": false, "product": "ribonucleoside-diphosphate reductase subunit alpha", "ec_ids": [ "1.17.4.1" ], "uniref50_id": "UniRef50_UPI0021AFF10F", "go_ids": [ "GO:0004748", "GO:0005971", "GO:0009265" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0021AFF10F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021AFF10F" ], "product": "class 1b ribonucleoside-diphosphate reductase subunit alpha" }, "genes": [], "id": "APKECPOBEN_711", "locus": "GCA002090215_00684" }, { "type": "cds", "sequence": "contig_1", "start": 385353, "stop": 385784, "strand": "+", "frame": 3, "gene": null, "product": "Ribonucleotide-diphosphate reductase subunit alpha", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A655JDN6" ], "nt": "TTGGCCAATTCCCACTTACTGGCAATTGCTCCAACGGGTTCAATTTCCTATCTCTCCTCCTGTACTCCTTCACTCCAACCGGTAGTATCCCCGGTAGAAGTGCGCAAGGAAGGTCGGTTGGGACGGATTTATGTACCGGCTTACCAACTCAATAAAGACAGTTACCCTTTTTATAAAGATGGTGCTTACGAACTGGGTCCCGAACCAATTATTAACATAGCAGCAGCGGCACAACAACACGTCGATCAAGCGATTTCGCTCACCCTATTTATGACTGATAAGGCTACCACTCGTGACCTCAATAAAGCTTATATTTATGCTTTTAAAAAGGGTTGTTCTTCCATTTACTATGTCCGAGTGCGCCAGGAAGTACTAGAGGACAGTGAGGATCACACCATTCAAATGCAGCAGTGTGAGGCCTGTGTGATTTAA", "aa": "MANSHLLAIAPTGSISYLSSCTPSLQPVVSPVEVRKEGRLGRIYVPAYQLNKDSYPFYKDGAYELGPEPIINIAAAAQQHVDQAISLTLFMTDKATTRDLNKAYIYAFKKGCSSIYYVRVRQEVLEDSEDHTIQMQQCEACVI", "aa_hexdigest": "fb6271ea63a8efc0623df6849ecd1699", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A655JDN6", "query_cov": 0.986013986013986, "subject_cov": 0.9929577464788732, "identity": 0.518, "score": 147.0, "evalue": 4.24e-43, "valid": false, "product": "Ribonucleotide-diphosphate reductase subunit alpha", "uniref50_id": "UniRef50_A0A655JDN6" }, "pscc": { "uniref50_id": "UniRef50_A0A655JDN6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A655JDN6" ], "product": "Ribonucleotide-diphosphate reductase subunit alpha" }, "genes": [], "id": "APKECPOBEN_712", "locus": "GCA002090215_00685" }, { "type": "cds", "sequence": "contig_1", "start": 385931, "stop": 386233, "strand": "+", "frame": 2, "gene": "rplU", "product": "50S ribosomal protein L21", "db_xrefs": [ "COG:COG0261", "COG:J", "GO:0003723", "GO:0003735", "GO:0005737", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904", "KEGG:K02888", "RefSeq:WP_010874681.1", "SO:0001217", "UniParc:UPI0000133D48", "UniRef:UniRef100_P78026", "UniRef:UniRef50_P47474", "UniRef:UniRef90_P78026" ], "nt": "ATGCATGCTATTGTAGTCTGTGGATCAAAGCAATATTTAGTCCACGAAAATGACACTTTTTTTGTTGAAAAACTTGAAGCGCCAGTTGGTAAAGAAATCCAACTCGACAAGGTTTTAATGTTGGATGAAAAGATTGGTGCGCCATACTTGGAAAAAGCGCGAGTAGTGTGTGTGGTGGAAAAGCACGGTTTACAACGCAAAGTAAACGTGATTAAACACATCTCACAAAAACACCACTTAAAAAAGTATGGTCACCGTCAACCTTACACTAAGTTAAAGGTAGTGCGCTTTGTCCATGATTAA", "aa": "MHAIVVCGSKQYLVHENDTFFVEKLEAPVGKEIQLDKVLMLDEKIGAPYLEKARVVCVVEKHGLQRKVNVIKHISQKHHLKKYGHRQPYTKLKVVRFVHD", "aa_hexdigest": "f4fd694f151cc62406782937221bdfff", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000133D48", "ncbi_nrp_id": "WP_010874681.1", "uniref100_id": "UniRef100_P78026", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000133D48", "RefSeq:WP_010874681.1", "UniRef:UniRef100_P78026" ] }, "ips": { "uniref100_id": "UniRef100_P78026", "uniref90_id": "UniRef90_P78026" }, "psc": { "uniref90_id": "UniRef90_P78026", "gene": "rplU", "product": "50S ribosomal protein L21", "uniref50_id": "UniRef50_P47474", "cog_id": "COG0261", "cog_category": "J", "kegg_orthology_id": "K02888", "go_ids": [ "GO:0003723", "GO:0003735", "GO:0005737", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_P47474", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47474" ], "product": "Large ribosomal subunit protein bL21" }, "genes": [ "rplU" ], "id": "APKECPOBEN_713", "locus": "GCA002090215_00686" }, { "type": "cds", "sequence": "contig_1", "start": 386226, "stop": 386528, "strand": "+", "frame": 3, "gene": "prp", "product": "Ribosomal processing cysteine protease Prp", "db_xrefs": [ "COG:COG2868", "COG:S", "EC:3.4.22.-", "GO:0006508", "GO:0008234", "GO:0042254", "KEGG:K07584", "RefSeq:WP_010874682.1", "SO:0001217", "UniParc:UPI00001394CB", "UniRef:UniRef100_P75459", "UniRef:UniRef50_P75459", "UniRef:UniRef90_P75459" ], "nt": "ATGATTAAACTAACTGTGTCGCACCACAAACTCACCGCTTCCGGGCACGCTTTATTTGCCAAAAAGGGGCAAGACATTGTGTGCGCTGCTGTTAGTGGTATTATCTTTGGCGCTTTGCCCTGGTTTGAAACCAACAGCATTGCCGTTCAAGAAGATGCTACTGTACCCAGTTTAAGCTTGGAGTTAGTGCAACCAACCGCTAAGCTTATCACGGGATTGAGTGTGGTGATTATGCAACTTAAAACCCTCGCGCATTCTTACCCGCAGTTTATTTCGTTTGAAGATCAACGTAAAGATGAATAA", "aa": "MIKLTVSHHKLTASGHALFAKKGQDIVCAAVSGIIFGALPWFETNSIAVQEDATVPSLSLELVQPTAKLITGLSVVIMQLKTLAHSYPQFISFEDQRKDE", "aa_hexdigest": "74427003d8133d4a4fdf9c3971842635", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001394CB", "ncbi_nrp_id": "WP_010874682.1", "uniref100_id": "UniRef100_P75459", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001394CB", "RefSeq:WP_010874682.1", "UniRef:UniRef100_P75459" ] }, "ips": { "uniref100_id": "UniRef100_P75459", "uniref90_id": "UniRef90_P75459" }, "psc": { "uniref90_id": "UniRef90_P75459", "gene": "prp", "product": "Ribosomal processing cysteine protease Prp", "ec_ids": [ "3.4.22.-" ], "uniref50_id": "UniRef50_P75459", "cog_id": "COG2868", "cog_category": "S", "kegg_orthology_id": "K07584", "go_ids": [ "GO:0008234", "GO:0006508", "GO:0042254" ] }, "pscc": { "uniref50_id": "UniRef50_P75459", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75459" ], "product": "Ribosomal processing cysteine protease Prp" }, "genes": [ "prp" ], "id": "APKECPOBEN_714", "locus": "GCA002090215_00687" }, { "type": "cds", "sequence": "contig_1", "start": 386521, "stop": 386835, "strand": "+", "frame": 1, "gene": "rpmA", "product": "50S ribosomal protein L27", "db_xrefs": [ "GO:0000315", "GO:0003735", "GO:0006412", "GO:0022625", "KEGG:K02899", "SO:0001217", "UniParc:UPI0000133E0A", "UniRef:UniRef100_P75458", "UniRef:UniRef50_P05657", "UniRef:UniRef90_P75458" ], "nt": "ATGAATAACAAATATTTTTTAACCAAAATTGACTTACAGTTTTTTGCTTCCAAAAAGGGAGTTGGTTCAACCAAGAACGGCCGTGATTCTCATGCTAAGCGTTTAGGTGCTAAGAAGGCTGATGGTCAGATGATCCGTACTGGTCAAATTATTTACCGCCAACGCGGTACTAGGGTATATCCCGGTGTAAATGTTGGCCTTGGTAGTGATGACACCTTGTTTGCTTTAAGCGACGGCTTGGTAAAATACCAAAAGTTTGGACCTAAACAGGGCAAGACCCGCGTGAGTGTGGTCAAACACAAACTAGATGCCTAA", "aa": "MNNKYFLTKIDLQFFASKKGVGSTKNGRDSHAKRLGAKKADGQMIRTGQIIYRQRGTRVYPGVNVGLGSDDTLFALSDGLVKYQKFGPKQGKTRVSVVKHKLDA", "aa_hexdigest": "6e5d8a0e208b7b8aeada86d4cbe8eb69", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000133E0A", "uniref100_id": "UniRef100_P75458", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000133E0A", "UniRef:UniRef100_P75458" ] }, "ips": { "uniref100_id": "UniRef100_P75458", "uniref90_id": "UniRef90_P75458" }, "psc": { "uniref90_id": "UniRef90_P75458", "gene": "rpmA", "product": "50S ribosomal protein L27", "uniref50_id": "UniRef50_P05657", "kegg_orthology_id": "K02899", "go_ids": [ "GO:0000315", "GO:0003735", "GO:0006412", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_P05657", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P05657" ], "product": "Large ribosomal subunit protein bL27" }, "genes": [ "rpmA" ], "id": "APKECPOBEN_715", "locus": "GCA002090215_00688" }, { "type": "cds", "sequence": "contig_1", "start": 386828, "stop": 387673, "strand": "+", "frame": 2, "gene": "nfo", "product": "putative endonuclease 4", "db_xrefs": [ "COG:COG0648", "COG:L", "EC:3.1.21.2", "GO:0003677", "GO:0003906", "GO:0006284", "GO:0008081", "GO:0008270", "GO:0008833", "SO:0001217", "UniRef:UniRef50_P47477", "UniRef:UniRef90_P75457" ], "nt": "ATGCCTAAACTCCTAGGAAGCTTTATTTCGTTTAAATCACCCCATTATCTAGTGGGGAGTGTTCGTGACGCTGTTAGCATTAAAGCACAAGCGTTCATGATCTTTTTGGGCGCACCACACACGGCACTACGAGTCGATCCTAATCGTATGCAAATTGACGAGGGTCACACATTGATGGAACAACATAACCTCAGTAAGTCTGGGATGGTTGTCCATGCTCCTTACATTATTAACTGTGCCTCCAAAGATCCGGTAAAGCAAACCTTTGCGATTGACGTTCTGACAAGGGAGGTTAAACTGTGTCATGCCGTGGGTGCTAAACTAATCGTTCTCCACCCCGGTTCAGCGGTTGAGCAAACTCAAACCCAAGCGTTAGATCACCTAATCAAGGTCTTAAATACAGTGATTGCCAACACTAAAGAAGTCATTATTTGTCTCGAAACGATGGCTGGTAAAGGTAATGAAATTGGCCGTGATTTAGACCAGTTAAAGTATGTGATTAACCACATTGAACAACAAGAGCGGATTGGTGTTTGTCTAGATACCTGTCACTTCCATGACAGTGGTAATGACTTTAACAATACTGCCGAAATTATGGAAACGATTGACACTAAACTAGGCTTTGAGTTTTTAAAGGTCATCCACTTAAACGAGTCCAAAAACGTTTGTGGTTCCAAAAAAGACCGTCATGCCAATCTAGGTGAGGGGATGATTGGGTTTGATAATTTAATGCGCTTTATTGCCCAACCGCAAATAAAGCAAATACCGATTGTTTTAGAAACCCCATCTGACAAGCATAATTATCCCGCTGTATATGGTGCTGAAATCGAAAGGATCCGTGCTTGA", "aa": "MPKLLGSFISFKSPHYLVGSVRDAVSIKAQAFMIFLGAPHTALRVDPNRMQIDEGHTLMEQHNLSKSGMVVHAPYIINCASKDPVKQTFAIDVLTREVKLCHAVGAKLIVLHPGSAVEQTQTQALDHLIKVLNTVIANTKEVIICLETMAGKGNEIGRDLDQLKYVINHIEQQERIGVCLDTCHFHDSGNDFNNTAEIMETIDTKLGFEFLKVIHLNESKNVCGSKKDRHANLGEGMIGFDNLMRFIAQPQIKQIPIVLETPSDKHNYPAVYGAEIERIRA", "aa_hexdigest": "62a774368a256e0f18ae38b14afee857", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75457", "query_cov": 1.0, "subject_cov": 0.9825174825174825, "identity": 1.0, "score": 566.0, "evalue": 6e-204, "valid": true, "gene": "nfo", "product": "Probable endonuclease 4", "ec_ids": [ "3.1.21.2" ], "uniref50_id": "UniRef50_P47477", "cog_id": "COG0648", "cog_category": "L", "go_ids": [ "GO:0008833", "GO:0003677", "GO:0003906", "GO:0008081", "GO:0008270", "GO:0006284" ] }, "pscc": { "uniref50_id": "UniRef50_P47477", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47477" ], "product": "Probable endonuclease 4" }, "genes": [ "nfo" ], "id": "APKECPOBEN_716", "locus": "GCA002090215_00689" }, { "type": "cds", "sequence": "contig_1", "start": 387642, "stop": 388118, "strand": "+", "frame": 3, "gene": "fur", "product": "putative MG236-like protein", "db_xrefs": [ "COG:COG0735", "COG:P", "GO:0000976", "GO:0003700", "GO:0008270", "GO:0045892", "GO:1900376", "RefSeq:WP_010874685.1", "SO:0001217", "UniParc:UPI00001394D1", "UniRef:UniRef100_P75456", "UniRef:UniRef50_P75456", "UniRef:UniRef90_P75456" ], "nt": "ATGGTGCTGAAATCGAAAGGATCCGTGCTTGATTTGGAGCACGATAACCAAACACTAAAGGATTACATTAGTGTGTTTGAACGCAATAAGATGCGTTTAACCCAATCACGCTTAATGTTGCTCCAATGCTTAGTGCAACACCGTGACTGGCACACCTTAGCTGAGCTCAAACACCACTTGGAACAAAACCAACAGCGCACTACCTTAGCTTCTATCTATAACAACCTCAAGATTTTTGCTAAGTTAAAGCTAATTAATATTTTTGTAGATACCAATCGCTTTGAAACGTATTATTGCTTAAGACACGAAAACCACAAGCACATTTACTTCTTTGACGAAAACCAGCGCAAGTTTTTAACCCTACCACTACAAGACAAGGAAGCGTTGAGTTTAATTGGACATAAAAGTAAACATGGCAAAATTAAACTGAATGACTTTTACATAGTCGCTAGCGGTACACTTGAAGATGATCAATAA", "aa": "MVLKSKGSVLDLEHDNQTLKDYISVFERNKMRLTQSRLMLLQCLVQHRDWHTLAELKHHLEQNQQRTTLASIYNNLKIFAKLKLINIFVDTNRFETYYCLRHENHKHIYFFDENQRKFLTLPLQDKEALSLIGHKSKHGKIKLNDFYIVASGTLEDDQ", "aa_hexdigest": "6ae7dda45d01c26158f5d460a185332a", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001394D1", "ncbi_nrp_id": "WP_010874685.1", "uniref100_id": "UniRef100_P75456", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001394D1", "RefSeq:WP_010874685.1", "UniRef:UniRef100_P75456" ] }, "ips": { "uniref100_id": "UniRef100_P75456", "uniref90_id": "UniRef90_P75456" }, "psc": { "uniref90_id": "UniRef90_P75456", "gene": "Fur", "product": "Uncharacterized protein MG236 homolog", "uniref50_id": "UniRef50_P75456", "cog_id": "COG0735", "cog_category": "P", "go_ids": [ "GO:0003700", "GO:0000976", "GO:0008270", "GO:0045892", "GO:1900376" ] }, "pscc": { "uniref50_id": "UniRef50_P75456", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75456" ], "product": "Uncharacterized protein MG236 homolog" }, "genes": [ "fur" ], "id": "APKECPOBEN_717", "locus": "GCA002090215_00690" }, { "type": "cds", "sequence": "contig_1", "start": 388108, "stop": 388992, "strand": "+", "frame": 1, "gene": null, "product": "putative MG237-like protein", "db_xrefs": [ "RefSeq:WP_010874686.1", "SO:0001217", "UniParc:UPI00001394D4", "UniRef:UniRef100_P75455", "UniRef:UniRef50_P75455", "UniRef:UniRef90_P75455" ], "nt": "ATGATCAATAAGCCAAATCAGTTTGTCAACCACTTAAGTGCTTTAAAAAAACACTTTGCCTCTTATAAGGAGCTCCGTGAAGCGTTTAACGATTATCACAAGCATAACGGTGATGAGTTGACCACCTTCTTTTTGCACCAGTTTGATAAGGTGATGGAACTAGTCAAGCAGAAGGACTTTAAAACTGCCCAGTCTCGCTGTGAGGAGGAATTAGCCGCCCCTTATCTACCAAAACCTTTAGTAAGCTTTTTCCAATCGCTACTCCAGCTCGTGAACCATGATTTATTGGAACAGCAAAACGCGGCCTTGGCTAGTTTACCCGCAGCCAAAATCATAGAGCTTGTTTTACAGGATTACCCCAATAAACTCAACATGATTCACTACCTTTTGCCCAAAACGAAGGCGTTTGTCAAACCACATTTGTTACAACGCTTACAGTTTGTGTTAACTGATTCGGAGTTGTTGGAATTAAAGCGCTTTTCCTTCTTTCAAGCACTCAACCAGATCCCGGGCTTTCAGGGTGAGCAAGTTGAGTACTTTAACAGTAAGCTCAAACAAAAGTTTACCTTGACCTTAGGGGAGTTTGAAATTGCCCAACAGCCAGATGCCAAAGCATACTTTGAGCAGTTAATTACCCAAATCCAACAGCTCTTTTTAAAAGAGCCGGTTAACGCTGAGTTTGCCAATGAGATTATTGATGCCTTTTTGGTGAGCTATTTCCCCCTCCATCCCCCAGTACCTTTGGCACAATTAGCTGCCAAGATTTATGAGTATGTCAGCCAAATTGTGCTTAACGAAGCGGTTAACCTAAAGGATGAACTGATCAAACTAATCGTTCATACGCTTTACGAACAGTTAGATCGTCCTGTTGGGGATGAAAATTAA", "aa": "MINKPNQFVNHLSALKKHFASYKELREAFNDYHKHNGDELTTFFLHQFDKVMELVKQKDFKTAQSRCEEELAAPYLPKPLVSFFQSLLQLVNHDLLEQQNAALASLPAAKIIELVLQDYPNKLNMIHYLLPKTKAFVKPHLLQRLQFVLTDSELLELKRFSFFQALNQIPGFQGEQVEYFNSKLKQKFTLTLGEFEIAQQPDAKAYFEQLITQIQQLFLKEPVNAEFANEIIDAFLVSYFPLHPPVPLAQLAAKIYEYVSQIVLNEAVNLKDELIKLIVHTLYEQLDRPVGDEN", "aa_hexdigest": "e70a6b3f75a97b33ae1981c5cba6968a", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001394D4", "ncbi_nrp_id": "WP_010874686.1", "uniref100_id": "UniRef100_P75455", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001394D4", "RefSeq:WP_010874686.1", "UniRef:UniRef100_P75455" ] }, "ips": { "uniref100_id": "UniRef100_P75455", "uniref90_id": "UniRef90_P75455" }, "psc": { "uniref90_id": "UniRef90_P75455", "product": "Uncharacterized protein MG237 homolog", "uniref50_id": "UniRef50_P75455" }, "pscc": { "uniref50_id": "UniRef50_P75455", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75455" ], "product": "Uncharacterized protein MG237 homolog" }, "genes": [], "id": "APKECPOBEN_718", "locus": "GCA002090215_00691" }, { "type": "cds", "sequence": "contig_1", "start": 389140, "stop": 390351, "strand": "+", "frame": 1, "gene": "tig", "product": "trigger factor", "db_xrefs": [ "COG:COG0544", "COG:O", "EC:5.2.1.8", "GO:0003755", "GO:0005737", "GO:0005854", "GO:0006457", "GO:0015031", "GO:0051082", "GO:0051083", "GO:0051301", "GO:0140839", "GO:0140840", "SO:0001217", "UniRef:UniRef50_P47480", "UniRef:UniRef90_P75454" ], "nt": "ATGAAAATCAAAGGTTTTCGTAAAGGTAAAGTGCCTCCAACTTTAGCTAAGGATTACTTAGACCGTGCCGAGTTGTTGCAACGTAGCGCCCAAGCGGTAATTGATGCCATCTTCCAGCCGTTACAACAAGAAGCAGTAATTGCTGACAACGAGAATGTAATTGAGGACTTTCCTACCATTGACTTTAAAACAATCAATGAGAACGATTGTGTTATTCTGTTTGACTTTGATCTCGTACCCCAGTTTGAACCACCTGATTACAAGCACATCAAGGATTTAAGCCCGATTGTACCCCTAAAGGATGAGGAGTTCAACAAGGAGTTGCACAACATCGAAAAGAACAAAGGGAAACTAGTTGATGTTAGTGACAAAGCATTGGCCAACAATGATATTGCCGTCATTGACTTTGTTGGCAAGGTAGATGGTAAGGTGCTTGAAAGCGCTACTGCCAAACAATACGAATTAACGATTGGCTCCAACAGCTTTATTGATGGCTTTGAAAGTGGTTTAATTGGCATGAAAGTAGGGGACAAGCGTCAGTTAAAGTTAAAGTTCCCCAAGGACTACCATGCTGAAGAATTGAAGGGTAAACCAGTAGAGTTTGACATAGAGCTCAAAGCAATTAAGCAGTTAGAGATCACCCCCATGGATGAAACTAACTTTAAGGAGTATTTACCCGCACAATACCAGGGTTTCAACTCACTCAAGGAATTTAAAACTTACTTTCATAAGTTAGTTTCCGCTAAGAAACTGGAAATTACCCTACAAGAAAACAGTGTTAAAATTCGCCAGTTCTTTTTAGCTAACACCACCTTACCTTACATTCCTGATTCCTTAATTAAATTGGAATCCGATCGCTTACTGAGAGCGCAAAAGGATCAAGCGGAGCAGTACAAGATTCCCTTTGAGAGATTGTTAGCAGCTTCCAAGCTTTCATTGGAGCAGTTGCAACAACGTAACATAAAAGAAGCACGTGATAACGTTACCTTTGCTTTAGTGATGAAACGAATCGCTGATGTGGAAAAAATCAAGGTTGATAACAAAAAGATTCACAGCGAAATCGAATCGATTATTGATGTTGAATACCCCTTTGTTAATGCCGAGTTGAAAAAACAAATGTTCCACAACATGGAACAACAAAAGGACTTTGTGGAATCGATAATTTTGAACCGTTTAACCACTACCAAGATAATTGAGTACTCCACTCATTAG", "aa": "MKIKGFRKGKVPPTLAKDYLDRAELLQRSAQAVIDAIFQPLQQEAVIADNENVIEDFPTIDFKTINENDCVILFDFDLVPQFEPPDYKHIKDLSPIVPLKDEEFNKELHNIEKNKGKLVDVSDKALANNDIAVIDFVGKVDGKVLESATAKQYELTIGSNSFIDGFESGLIGMKVGDKRQLKLKFPKDYHAEELKGKPVEFDIELKAIKQLEITPMDETNFKEYLPAQYQGFNSLKEFKTYFHKLVSAKKLEITLQENSVKIRQFFLANTTLPYIPDSLIKLESDRLLRAQKDQAEQYKIPFERLLAASKLSLEQLQQRNIKEARDNVTFALVMKRIADVEKIKVDNKKIHSEIESIIDVEYPFVNAELKKQMFHNMEQQKDFVESIILNRLTTTKIIEYSTH", "aa_hexdigest": "f4462b19c0cbaa9ffa68967848d09351", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75454", "query_cov": 1.0049627791563276, "subject_cov": 0.9080717488789237, "identity": 0.993, "score": 762.0, "evalue": 6.08e-277, "valid": true, "gene": "tig", "product": "trigger factor", "ec_ids": [ "5.2.1.8" ], "uniref50_id": "UniRef50_P47480", "cog_id": "COG0544", "cog_category": "O", "go_ids": [ "GO:0003755", "GO:0005737", "GO:0005854", "GO:0006457", "GO:0015031", "GO:0051082", "GO:0051083", "GO:0051301", "GO:0140839", "GO:0140840" ] }, "pscc": { "uniref50_id": "UniRef50_P47480", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47480" ], "product": "Trigger factor" }, "genes": [ "tig" ], "id": "APKECPOBEN_719", "locus": "GCA002090215_00692" }, { "type": "cds", "sequence": "contig_1", "start": 390422, "stop": 391402, "strand": "+", "frame": 2, "gene": null, "product": "LON peptidase substrate-binding domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F21836E", "UniRef:UniRef90_UPI001F21836E" ], "nt": "ATGCCAGCTGTAAAAAAACCACAAATTCTCGTTGTGCGTAACCAAGTTATCTTTCCGTACAACGGCTTTGAACTTGATGTCGGTCGTGAACGCTCGAAAAAACTAATTAAAGCACTCAAAAACCTCAAAACCAAACGTTTGGTGTTAGTCACCCAAAAGAATTCAGATCAGCTCAACCCTGAGTTTGATGACATTTACCATTGCGGTACTTTATGTGACATTGATGAAATTATTGAAGTACCGAGTGAAGATGGCAAGACAGCTGACTATAAGATTAAGGGTAAAGGATTACAGCGCGTTGCCATTACAAGCTTCTCTGATGCTGATTTAACCAAGTACGACCATCACTTTCTCAACTCTACCCTAACAGAAAATAAAGCGTTAGATAAGTTGTTGGAACGGATCTTTCCCGATAAAGAAGACTTTGCTGAAATTTTAGACAGCTTAAACAGCTTCTTGGAACTGCAAGAGTTAAAGAAGCTATCTAAGGTACCAAAAGACATAAAACGGTATGACATTATTACCTTCAAGCTTGCTAGCTTAATTTTCAAAGACATTACCCTCCAACAAGCCATTTTGGAAGAAAATGACATTGAAAAACGGTTACAGAAGATCATTGGTTCTGGCATCGAGGATTTAGGTCACATCAGCGAAGAAGCCCGCGCTAAACAACGCGAAAGTGAGTTTGACAAGATTGACAACCGCATCACGCGCAAAGTTAACGAACAGTTATCGCGCCAACAACGTGACTTTTACCTCCGGGAAAAACTCCGGGTTATCCGTGAAGAGATCGGGATGACTTCCAAGAAGGAAGATGAAGTATCCAACATTCGCAAAAAGCTAGAGGAAAACCCATATCCAGAGCACATTAAAAAACGGATTTTAAGTGAGTTAGATCACTTTGAAAACTCCTCCTCTTCATCACAAGAATCAACCTTAACCAAGACTTACATCGACACTTTGATGAACTTGCCATGGTGA", "aa": "MPAVKKPQILVVRNQVIFPYNGFELDVGRERSKKLIKALKNLKTKRLVLVTQKNSDQLNPEFDDIYHCGTLCDIDEIIEVPSEDGKTADYKIKGKGLQRVAITSFSDADLTKYDHHFLNSTLTENKALDKLLERIFPDKEDFAEILDSLNSFLELQELKKLSKVPKDIKRYDIITFKLASLIFKDITLQQAILEENDIEKRLQKIIGSGIEDLGHISEEARAKQRESEFDKIDNRITRKVNEQLSRQQRDFYLREKLRVIREEIGMTSKKEDEVSNIRKKLEENPYPEHIKKRILSELDHFENSSSSSQESTLTKTYIDTLMNLPW", "aa_hexdigest": "f5dded132c55a2ffa45747c08e59f906", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001F21836E", "query_cov": 0.8006134969325154, "subject_cov": 1.0, "identity": 0.985, "score": 490.0, "evalue": 1.52e-173, "valid": true, "product": "LON peptidase substrate-binding domain-containing protein", "uniref50_id": "UniRef50_UPI001F21836E" }, "pscc": { "uniref50_id": "UniRef50_UPI001F21836E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F21836E" ], "product": "LON peptidase substrate-binding domain-containing protein" }, "genes": [], "id": "APKECPOBEN_720", "locus": "GCA002090215_00693" }, { "type": "cds", "sequence": "contig_1", "start": 391448, "stop": 392809, "strand": "+", "frame": 2, "gene": "lon", "product": "endopeptidase La", "db_xrefs": [ "COG:COG0466", "COG:O", "EC:3.4.21.53", "GO:0004176", "GO:0004252", "GO:0005524", "GO:0005737", "GO:0006508", "GO:0006515", "GO:0016887", "GO:0034605", "GO:0043565", "SO:0001217", "UniRef:UniRef50_P47481", "UniRef:UniRef90_P78025" ], "nt": "TTGAACAAGAACCACTCAGGACTAGATAAGGTAAAGGAACGAGTGGTTGAGTATTTAGCAGTACAGCTCCGTACCAAAAAGCTTAAGGGTCCGATTATGTGTTTAGTCGGTCCACCTGGAGTTGGTAAATCCAGTTTAGCCAAATCAATTGCTGAAGCGTTAAACAAGTGCTTTGTTAAGGTTTCATTGGGCGGGGTGCATGATGAATCGGAAATCCGTGGTCACCGTAAAACCTATTTGGGTTCGATGCCAGGACGGATTTTAAAGGGCATGGTACGTGCTAAGGTTATTAACCCACTGTTCTTGTTAGATGAAATTGACAAGATGACATCCTCCAATCAAGGTTACCCTTCCGGCGCTTTGTTAGAGGTATTAGATCCGGAGTTAAACAATAAGTTTAGCGATAACTATGTTGAGGAGGATTATGACCTGTCTAAGGTAATGTTTGTAGCCACCGCTAACTATATCGAAGATATTCCGGAAGCCTTGTTAGACCGGATGGAAGTGATTGAACTTACCTCTTACACCGAACAGGAAAAGCTACAAATTACAAAGAGCCACTTAGTTAAGCGTTGTTTAGATGACGCTGAGATTAAAACAGATGACCTCAAGTTTACCGATGAGGGTATTAGCTATATCATTAAGTTTTACACGAGGGAAGCGGGGGTGAGACAGTTAGAACGCTTAATCCAGCAAATTGTGCGTAAGTACATTGTTAACTTGCAAAAAACCGGCGAACAACAGGTAGTAGTTGATGTAGATTTGGTAAAGAAATACCTCAAGAAAGAAATCTTTGACTATACCGTGCGCGATGAAGATGCACTTCCGGGAATTGTTAACGGCATGGCTTACACCCCAACTGGTGGTGATCTGTTACCAATTGAAGTAACCCATGTAGCTGGTAAGGGTGATTTAATTCTGACCGGCAATTTAAAGCAAACAATGCGTGAAAGTGCTAGCGTAGCGTTAGGTTATGTCAAGGCCAATGCGCAGAGCTTTAACATTAACCCTAACCTCTTTAAAAAGGTTGACATTAACATCCATGTTCCAGGTGGTGGTATTCCCAAGGATGGCCCGAGTGCTGGCGCGGCTTTAGTTACTGCCATTATCTCGTCTTTAACTGGCAAAAAGGTTGACCCTAAGATTGCCATGACCGGGGAGATTACCCTAAGGGGTAAAGTAATGACCATAGGCGGGGTGAAAGAAAAAACCATTTCCGCCTACCGCGGTGGAGTACGCACCATCTTTATGCCCGAAAAGAATGAGCGTTACTTAGATGAAGTGCCTAAAGACATCGTTAAGGACTTGGAGATTATCCTAGTAAAGGAATACAAGGACATCTACAACAAGATATTTAATTAA", "aa": "MNKNHSGLDKVKERVVEYLAVQLRTKKLKGPIMCLVGPPGVGKSSLAKSIAEALNKCFVKVSLGGVHDESEIRGHRKTYLGSMPGRILKGMVRAKVINPLFLLDEIDKMTSSNQGYPSGALLEVLDPELNNKFSDNYVEEDYDLSKVMFVATANYIEDIPEALLDRMEVIELTSYTEQEKLQITKSHLVKRCLDDAEIKTDDLKFTDEGISYIIKFYTREAGVRQLERLIQQIVRKYIVNLQKTGEQQVVVDVDLVKKYLKKEIFDYTVRDEDALPGIVNGMAYTPTGGDLLPIEVTHVAGKGDLILTGNLKQTMRESASVALGYVKANAQSFNINPNLFKKVDINIHVPGGGIPKDGPSAGAALVTAIISSLTGKKVDPKIAMTGEITLRGKVMTIGGVKEKTISAYRGGVRTIFMPEKNERYLDEVPKDIVKDLEIILVKEYKDIYNKIFN", "aa_hexdigest": "fca27085787feaf06508350604fd696c", "start_type": "TTG", "rbs_motif": "AAAA", "psc": { "uniref90_id": "UniRef90_UPI0025901479", "query_cov": 0.9867549668874173, "subject_cov": 0.8799212598425197, "identity": 0.523, "score": 491.0, "evalue": 5.86e-168, "valid": false, "gene": "lon", "product": "endopeptidase La", "ec_ids": [ "3.4.21.53" ], "uniref50_id": "UniRef50_A0A7C6Y0Z4", "go_ids": [ "GO:0004252", "GO:0005524", "GO:0006508", "GO:0016887" ] }, "genes": [ "lon" ], "truncated": "5-prime", "pseudogene": { "start": 391148, "stop": 392809, "observations": { "mutation": [ 391400 ], "stop": [ 391400 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P78025", "query_cov": 1.0, "subject_cov": 0.5490909090909091, "identity": 0.993, "score": 874.0, "evalue": 2.28e-314, "gene_start": 1, "gene_end": 453, "reference_start": 373, "reference_end": 825, "reference_sequence": "MSTPLISTQSIRVLKNVLQFSILQPLILITMPAVKKPQILVVRNQVIFPYNGFELDVGRERSKKLIKALKNLKTKRLVLVTQKNSDQLNPEFDDIYHCGTLCDIDEIIEVPSEDGKTADYKIKGKGLQRVAITSFSDADLTKYDHHFLNSTLTENKALDKLLERIFPDKEDFAEILDSLNSFLELQELKKLSKVPKDIKRYDIITFKLASLIFKDITLQQAILEENDIEKRLQKIIGSGIEDLGHISEEARAKQRESEFDKIDNRITRKVNEQLSRQQRDFYLREKLRVIREEIGMTSKKEDEVSNIRKKLEENPYPEHIKKRILSELDHFENSSSSSQESTLTKTYIDTLMNLPWWQESKDNADVKNLIKILNKNHSGLDKVKERVVEYLAVQLRTKKLKGPIMCLVGPPGVGKSSLAKSIAEALNKRFVKVSLGGVHDESEIRGHRKTYLGSMPGRILKGMARAKVINPLFLLDEIDKMTSSNQGYPSGALLEVLDPELNNKFSDNYVEEDYDLSKVMFVATANYIEDIPEALLDRMEVIELTSYTEQEKLQITKSHLVKRCLDDAEIKTDDLKFTDEGISYIIKFYTREAGVRQLERLIQQIVRKYIVNLQKTGEQQVVVDVDLVKKYLKKEIFDYTVRDEDALPGIVNGMAYTPTGGDLLPIEVTHVAGKGDLILTGNLKQTMRESASVALGYVKANAQSFNINPNLFKKVDINIHVPGGGIPKDGPSAGAALVTAIISSLTGKKVDPKIAMTGEITLRGKVMTIGGVKEKTISAYRGGVRTIFMPEKNERYLDEVPKDIVKDLEIILVKEYKDIYNKIFN" }, "paralog": false, "identity": 0.9945750452079566, "score": 1073.0, "evalue": 0.0, "description": "Internal stop codon at 391400. Nonsense mutation around 391400.", "psc": { "uniref90_id": "UniRef90_P78025", "gene": "lon", "product": "endopeptidase La", "ec_ids": [ "3.4.21.53" ], "uniref50_id": "UniRef50_P47481", "cog_id": "COG0466", "cog_category": "O", "go_ids": [ "GO:0004176", "GO:0004252", "GO:0005524", "GO:0005737", "GO:0006508", "GO:0006515", "GO:0016887", "GO:0034605", "GO:0043565" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P78025", "EC:3.4.21.53", "UniRef:UniRef50_P47481", "COG:COG0466", "COG:O", "GO:0004176", "GO:0004252", "GO:0005524", "GO:0005737", "GO:0006508", "GO:0006515", "GO:0016887", "GO:0034605", "GO:0043565" ] }, "pscc": { "uniref50_id": "UniRef50_P47481", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47481" ], "product": "Lon protease" } }, "id": "APKECPOBEN_721", "locus": "GCA002090215_00694" }, { "type": "cds", "sequence": "contig_1", "start": 393121, "stop": 393660, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF12679.13" ], "nt": "GTGACACTGTTAGCGGGCAGTATTACCGCTGACATCTTTATTGAAGGGTACAACAACGGGATTGAGCTGTTCTTGTTCTCTAAACCTATTAGCCGTGACCGCATTTTGCTCATTAAGTTACTAGTGCTGTTAATGTACTTGAGTATTGTAGCCGTACTATCCACTGCTGTAGTGAGCTTGGGTTACTTTACGCGCAATGAGATTGAGAGTTATTTAGATGATTTAGCTTTAAGTATCTTTGTCGGTACCATTTTAAACGGCATTATCTTTAGCGCTGTAACTATTATGTTAGCCACGCGCTTTAGCAACATCCAGAGCTTAATCATTTTGCTCTTGTTTGTGTCGTTGTTTAGCTTTTCCTCACCAATTGCGCGGTTAATCTTCGACACGCCTGCTAAAACGTTGAGCCAACAAGGTTACAGCGTTCGCCAAATTAACGGAATTGCGCACAGCGAAAGCCCTAATGTAGAGCGTAAAATTTACGTTTCGGTCAAAAAGCGGCGTTTAACTGGGGTGGATCGCGAAGAAAAAGAGCTTTGA", "aa": "MTLLAGSITADIFIEGYNNGIELFLFSKPISRDRILLIKLLVLLMYLSIVAVLSTAVVSLGYFTRNEIESYLDDLALSIFVGTILNGIIFSAVTIMLATRFSNIQSLIILLLFVSLFSFSSPIARLIFDTPAKTLSQQGYSVRQINGIAHSESPNVERKIYVSVKKRRLTGVDREEKEL", "aa_hexdigest": "5603289a283a8d8264b129f90334c40b", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF12679.13", "name": "ABC2_membrane_2", "length": 127, "aa_cov": 0.6983240223463687, "hmm_cov": 0.9921259842519685, "evalue": 9.601028192766301e-10, "score": 33.584739685058594, "start": 3, "stop": 127 } ], "seq_stats": { "molecular_weight": 19968.253999999997, "isoelectric_point": 8.92314968109131 }, "id": "APKECPOBEN_722", "locus": "GCA002090215_00695" }, { "type": "cds", "sequence": "contig_1", "start": 393697, "stop": 394104, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTATGAGGAGGCACAACGAACCTCCTCTTACATTAATTCCTTTTACACCAACATTGGTTACTTATTTGGCTCGCTTTACCGCTTGGGTGCGTTAGCTGATGAAAATAACTTTGATGCATCAGTAGCAACCCAAAACACCAAAATTCGCTTTGGTAAGTTAGTTGATTTAGATAAGAGTACTGACATTATTGGTTATCGCACTAAAGACCGCTTTGGCCGTTTCTTTCGCTTTCACTTGCGCTTTCCAACGCTCAACTTTAAGCAAAGCTTGAGTAACAGCGGCGTTTTCTTTATCAATAAACGGGGATTAGTTAAAGCTACTTTTAGTGCTGGCGATGATGCCTTTAAACGTGACCCGAAGATAATGCAAAAGGTGTTTGACACTTTTCTAGAAAGTTTTAGTTGA", "aa": "MYEEAQRTSSYINSFYTNIGYLFGSLYRLGALADENNFDASVATQNTKIRFGKLVDLDKSTDIIGYRTKDRFGRFFRFHLRFPTLNFKQSLSNSGVFFINKRGLVKATFSAGDDAFKRDPKIMQKVFDTFLESFS", "aa_hexdigest": "ecd16d1d22dc9eb0d09bd42a00d2966c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15598.45779999998, "isoelectric_point": 9.652222633361816 }, "id": "APKECPOBEN_723", "locus": "GCA002090215_00696" }, { "type": "cds", "sequence": "contig_1", "start": 394294, "stop": 394401, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCTAGTGAAAGCACTGCCACCGGTATTTCTAGTGATCCCCAAAGTCAGGGCAAAATGAACAACAAAAGTGAGGAACAAAAAAAGAAAGAAAAAGAACTCACTTGA", "aa": "MSSESTATGISSDPQSQGKMNNKSEEQKKKEKELT", "aa_hexdigest": "0601b64c4b95ea4f51e647d93046f2b4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3844.1550000000007, "isoelectric_point": 6.117035102844238 }, "id": "APKECPOBEN_724", "locus": "GCA002090215_00697" }, { "type": "cds", "sequence": "contig_1", "start": 394495, "stop": 395256, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAACGCTGAAGACCAAAACGGGGATAAAAACCAGTTTGCCCAAGCAATGGCATCACTCAATAAGCAAATTAACATGTTTTACGTGTTGAATGAAATTACCAACAAGGAACACAATTTGCCAACTAAATACGGTGCTTATTCTGATCAATTAAAAAAGGTCATAAACGAAAACTTTGAAAAGATAGAAAATAAGAAAAAGGAGATAGAGGAAAAACAAAATAAAGAATTTCAGGAAAACAAGAATGGTGCATCAAATAATCAAGACACAAAGCAAGACGCACAAAAAGGTGATACCAATACCCAAAGCACTGAGTTAAAAGCTCGTCAAGCACAACAAGTGCAAAAGGATCAACAAAATGACAGTAAAGGCAATACTGCCACAAACAGTGATACTGGTAAATCGGACAATAAAACCGATACCACTGAAGATGAACAAAACACATATAAACCCTTAACAGCTAGGGAGAAAAAAACCAAAATTAAGGAGCTTACTAAGGACATCTTCTTCTCTACAGCGCAGAACTATTTGTTCTTAGTGCAAGGTGGGTATAACAACAAGTTGTTTGCTGGTGGTTTACCAGAGCTCCACCAACGCTTCTTGTCAGACGTAGTGGTTGATTTCTCTGAGGAAAAACTCGTTTATGAAGTACAAGGTGAACCGATTGTATCCAGTGTAGCCACCATTGTGATTACCCTATTTTTAACAGTTGTTTTATTAGCTATTGCCTTTTTCTTCTTTAAATACCGCAACGTTAAATAG", "aa": "MNAEDQNGDKNQFAQAMASLNKQINMFYVLNEITNKEHNLPTKYGAYSDQLKKVINENFEKIENKKKEIEEKQNKEFQENKNGASNNQDTKQDAQKGDTNTQSTELKARQAQQVQKDQQNDSKGNTATNSDTGKSDNKTDTTEDEQNTYKPLTAREKKTKIKELTKDIFFSTAQNYLFLVQGGYNNKLFAGGLPELHQRFLSDVVVDFSEEKLVYEVQGEPIVSSVATIVITLFLTVVLLAIAFFFFKYRNVK", "aa_hexdigest": "aec4e83f697d997c4a50cacb8c9bb602", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 28880.81380000002, "isoelectric_point": 5.884847831726074 }, "id": "APKECPOBEN_725", "locus": "GCA002090215_00698" }, { "type": "cds", "sequence": "contig_1", "start": 395355, "stop": 395624, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00005.33" ], "nt": "ATGCCCGGTGAGTTCCATGCCTTTATTGGTCAAAATGGTGCGGGGAAAACTACCACCATTAAGTGTTTAATTTCATCTTATCAGCGCTTTAAGGACGAAATTAACATCGATGGCATTAGCAATAAAAACGCCAAGTCTAAGGGTGTAATTAGTTATATCCCTGAATATGCTGTGTTTCCCAAACACCTCAATACCCATGAATACCTCTACACGTTGGGTAAACTGTCAGGTTGTAGTTTGCAAACCATTAAGGAAAAGGTTGATTACTGA", "aa": "MPGEFHAFIGQNGAGKTTTIKCLISSYQRFKDEINIDGISNKNAKSKGVISYIPEYAVFPKHLNTHEYLYTLGKLSGCSLQTIKEKVDY", "aa_hexdigest": "e43cb07d3092f0b783e035908a936b46", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00005.33", "name": "ABC_tran", "length": 69, "aa_cov": 0.7415730337078652, "hmm_cov": 1.0, "evalue": 2.4017531145203677e-11, "score": 39.8638801574707, "start": 2, "stop": 67 } ], "seq_stats": { "molecular_weight": 9961.279999999993, "isoelectric_point": 8.887047386169435 }, "id": "APKECPOBEN_726", "locus": "GCA002090215_00699" }, { "type": "cds", "sequence": "contig_1", "start": 395742, "stop": 395996, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATGAGCCCACTGCTAACTTAGATCCCAAAACACGTAATGAGTTTATGGATGTGTGTTACGAACTTAATGTACGTAACAAGATGGCGGTGTTTGTTTCATCCCATATTTTGGCTGAATTGGAAAACTATTGTGATTCGCTCACTGTTATTCATGAAGGGGAAATTCTTTTTAATGGCAAAACAAAGAACATTGCTAAAGACAACTTTGGTTACCGTTTAAAGGTTAACAATTCCGATAGTTTACGTAAGTGA", "aa": "MDEPTANLDPKTRNEFMDVCYELNVRNKMAVFVSSHILAELENYCDSLTVIHEGEILFNGKTKNIAKDNFGYRLKVNNSDSLRK", "aa_hexdigest": "3861bb5b44c45e5732343c4feb0c973e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9669.854999999987, "isoelectric_point": 5.603381156921387 }, "id": "APKECPOBEN_727", "locus": "GCA002090215_00700" }, { "type": "cds", "sequence": "contig_1", "start": 396442, "stop": 397083, "strand": "+", "frame": 1, "gene": null, "product": "ABC transporter permease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001912123E", "UniRef:UniRef90_UPI001912123E" ], "nt": "ATGTCGAGTTTAATCATTCCTTTCATTGCTCTGTTTGCCACCATTATTACCTTTCAAGTCTTTATTAATGGTTATTACAACGGTCTAGAGATCTTATTAATTACCCGCTTTTTTACTCGCGGTCGGTTATTCTTTGCTCGCTTAGCAGTCTTGTTTATTTGGATTACTGGCACGGCATTTTTAAGTGGTCTTTTTGTTAGTTTAACTGCTACTTTAGGTGCTACATCACAAACAGTTACTGACCTTGTTTTAAGCGTCTTTTTTGGAGTGCTTTTACTTAGTTTATTGTTTAGCTGTGTCTTGATTATTATCGCGCAGTTTTTAAACCGCACCCAAAGTATGTTGTTGTTACTGTTAGGGGTTTCCCTAGCTAGCTTTACAACCGTTATCTTGGCCTTTTCAATTAAGCCACCGAGTGAAAATCTCCGTGATAATGGTTACCAACCACTAAACCTAAGTCTCATCAGTAAGTCTAAGTCCAAGAGTGTCGAAAATGTCTTTACCATCATTGACTCTTCAAATGTATCCGATCCGCGCAATAAAAAGAAATCGATTGTAAAAACCAATCCGCAAGCGATTTGGGATGAATATGGGGAGAGTAGCTTCTTTCAAAGCCAGTATTACTGGAATGTAGGGTATTGA", "aa": "MSSLIIPFIALFATIITFQVFINGYYNGLEILLITRFFTRGRLFFARLAVLFIWITGTAFLSGLFVSLTATLGATSQTVTDLVLSVFFGVLLLSLLFSCVLIIIAQFLNRTQSMLLLLLGVSLASFTTVILAFSIKPPSENLRDNGYQPLNLSLISKSKSKSVENVFTIIDSSNVSDPRNKKKSIVKTNPQAIWDEYGESSFFQSQYYWNVGY", "aa_hexdigest": "ba6e9218043e87a0a4579210673b8245", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 396226, "stop": 397386, "observations": { "mutation": [ 396244, 396325, 396334, 396421, 397081 ], "stop": [ 396244, 396325, 396334, 396421, 397081 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001912123E", "query_cov": 1.0, "subject_cov": 0.49882903981264637, "identity": 0.991, "score": 379.0, "evalue": 2.58e-129, "gene_start": 1, "gene_end": 213, "reference_start": 73, "reference_end": 285, "reference_sequence": "MKLFKNWSDVNNVKQRQLLLAYFRFILKQTVKSWLLWVTAGLVLFLLALVLLIIPAFTKQDPLFLWSHPVVQMSSLIIPFIALFATIITFQVFINGYYNGLEILLITRFFTRGRLFFARLAVLFIWITGTAFLSGLFVSLTATLGATSQTVTDLVLSVFFGVLLLSLLFSCVLIIIAQFLNRTQSMLLLLLGVSLASFTTVILAFSIKPPSENLRDNSYQPLNLSLISKSKSKGVENVFTIIDSSNVSDPRNKKKSIVKTNPQAIWDEYGESSFFQSQYYWNVGYWINSLFRLNSLSDYRNFDVNLYFLNTKLNFDREVDTDKMVDFVSFRDQRHLYFLSYSFFINTHNLPTQPLPRILTYDNNGAFLEKIVPSFDDIKLDPPRVKKVYKFFSDTIIQSFRDYESEEKERQEKEEKEKAEKDNGNGQ" }, "paralog": false, "identity": 0.9792746113989638, "score": 728.0, "evalue": 4.17e-269, "description": "Internal stop codon at 396244, 396325, 396334, 396421, 397081. Nonsense mutation around 396244, 396325, 396334, 396421, 397081.", "psc": { "uniref90_id": "UniRef90_UPI001912123E", "product": "ABC transporter permease", "uniref50_id": "UniRef50_UPI001912123E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001912123E", "UniRef:UniRef50_UPI001912123E" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001912123E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001912123E" ], "product": "ABC transporter permease" } }, "id": "APKECPOBEN_728", "locus": "GCA002090215_00701" }, { "type": "cds", "sequence": "contig_1", "start": 397195, "stop": 398451, "strand": "+", "frame": 1, "gene": "nosY", "product": "putative protein MPN_335", "db_xrefs": [ "COG:COG1277", "COG:O", "GO:0005886", "SO:0001217", "UniRef:UniRef50_P75443", "UniRef:UniRef90_P75443" ], "nt": "ATGGTCGACTTTGTTAGCTTTCGCGATCAAAGACACCTTTACTTTTTAAGTTATTCCTTTTTTATCAATACCCATAACCTGCCCACACAACCGTTACCCCGGATTCTTACTTATGACAATAACGGTGCCTTTTTAGAGAAAATAGTCCCTTCCTTTGATGACATAAAACTAGATCCACCCCGGGTCAAAAAGGTTTACAAATTCTTTAGTGACACCATTATCCAGTCCTTCCGTGATTATGAAGCGGAGGAAAAAGAAAGGCAGGAAAAGGAAGAGAAAGAAAAGGCTGAAAAGGACAATGGCAACGGCCAGGATTCCAATAAAGTTAACTCTGTCAGCACGGAGCCTAACAATAAGAATTCATCAGATGCGGATTCTAAGGATAACAATGATTCATCCGATTCCCAAGGCAAAGACTCAAGTAAGAGTAAGCCTAAGTTTAGACCTAGGCTGCCACAATTCTTTGATCGTGTTTCCATTAACTACAGTAAGTTTTCCAGTAGCTTTAACACACAACTAAAACACATTCATCAAGGACTTTCTACCCGTGATGAGGCCATGGAAATCTTTAAAGATAAGGTAGCGTTATTTTATGCCCTATCAATGGCTTATGACAACTTTACTTTCCAAAAGGACAGTGGCATTATTGATAACACTAGCTTAAACAAATTTAAAACAGAGTTTAACAAGAAGCAAAAAGAGCTAACTGAAAAAAACAAGCAAAAACAGGACGGTAAAGACCAAAAGAGTCAAAGAATGACACAAGGTGCTGATAAAGCAGTCACTGTTGAGCCAAAAGCTATGCAGTCTGAAATTGGAATGACTGATCAAACTAATGACCAAAGCAGTTCGGAAACAAAGGACAGCATGGATAGTTCAGACAGTAGTGATACTGTTGATAACACTGATGAATCAGAAGATAAACAAAGTGAAGAGGAGGAAAAGTTCGACGAGGAAATTGAGAATGCCAAAAAAATGCCTAAGGCTGAAGACGCCTTTTTTAATACTGCCTCTATCTGGTTGAGCTCACCATTTCTCTTTTTTGAAAATGGTGCAAAACGCGAACAACGATACGAAATCCATTTGCTCAATTCAAACACACAGGTTAATAACGAAATAATTAAAACTAACAGCTTTTATTACGTTAGTGGTGAACCGATTGTAGGCCAAGAAGTCATTATTGCAATGGTGCTGGTGGTAACACTAGGTCTGTTAGTTGGTAGTTTCTTCGCTTACCAAAAACGCGATATTAAGTAA", "aa": "MVDFVSFRDQRHLYFLSYSFFINTHNLPTQPLPRILTYDNNGAFLEKIVPSFDDIKLDPPRVKKVYKFFSDTIIQSFRDYEAEEKERQEKEEKEKAEKDNGNGQDSNKVNSVSTEPNNKNSSDADSKDNNDSSDSQGKDSSKSKPKFRPRLPQFFDRVSINYSKFSSSFNTQLKHIHQGLSTRDEAMEIFKDKVALFYALSMAYDNFTFQKDSGIIDNTSLNKFKTEFNKKQKELTEKNKQKQDGKDQKSQRMTQGADKAVTVEPKAMQSEIGMTDQTNDQSSSETKDSMDSSDSSDTVDNTDESEDKQSEEEEKFDEEIENAKKMPKAEDAFFNTASIWLSSPFLFFENGAKREQRYEIHLLNSNTQVNNEIIKTNSFYYVSGEPIVGQEVIIAMVLVVTLGLLVGSFFAYQKRDIK", "aa_hexdigest": "4804eeb1070233fb5a8adc7548ab2efd", "start_type": "ATG", "rbs_motif": null, "genes": [ "nosY" ], "truncated": "5-prime", "pseudogene": { "start": 396895, "stop": 398451, "observations": { "mutation": [ 397081 ], "stop": [ 397081 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75443", "query_cov": 1.0, "subject_cov": 0.5580774365821095, "identity": 1.0, "score": 592.0, "evalue": 9.55e-205, "gene_start": 1, "gene_end": 418, "reference_start": 332, "reference_end": 749, "reference_sequence": "MLLLSVNKMKLFKNWSDVNNVKQRQLLLAYFRFILKQTVKSWLLWVTAGLVLFLLALVLLIIPAFTKQDPLFLWSHPVVQMSSLIIPFIALFATIITFQVFINGYYNGLEILLITRFFTRGRLFFARLAVLFIWITGTAFLSGLFVSLTATLGATSQTVTDLVLSVFFGVLLLSLLFSCVLIIIAQFLNRTQSMLLLLLGVSLASFTTVILAFSIKPPSENLRDNSYQPLNLSLISKSKSKGVENVFTIIDSSNVSDPRNKKKSIVKTNPQAIWDEYGESSFFQSQYYWNVGYWINSLFRLNSLSDYRNFDVNLYFLNTKLNFDREVDTDKMVDFVSFRDQRHLYFLSYSFFINTHNLPTQPLPRILTYDNNGAFLEKIVPSFDDIKLDPPRVKKVYKFFSDTIIQSFRDYEAEEKERQEKEEKEKAEKDNGNGQDSNKVNSVSTEPNNKNSSDADSKDNNDSSDSQGKDSSKSKPKFRPRLPQFFDRVSINYSKFSSSFNTQLKHIHQGLSTRDEAMEIFKDKVALFYALSMAYDNFTFQKDSGIIDNTSLNKFKTEFNKKQKELTEKNKQKQDGKDQKSQRMTQGADKAVTVEPKAMQSEIGMTDQTNDQSSSETKDSMDSSDSSDTVDNTDESEDKQSEEEEKFDEEIENAKKMPKAEDAFFNTASIWLSSPFLFFENGAKREQRYEIHLLNSNTQVNNEIIKTNSFYYVSGEPIVGQEVIIAMVLVVTLGLLVGSFFAYQKRDIK" }, "paralog": false, "identity": 0.9961389961389961, "score": 793.0, "evalue": 2.84e-286, "description": "Internal stop codon at 397081. Nonsense mutation around 397081.", "psc": { "uniref90_id": "UniRef90_P75443", "gene": "NosY", "product": "Uncharacterized protein MPN_335", "uniref50_id": "UniRef50_P75443", "cog_id": "COG1277", "cog_category": "O", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75443", "UniRef:UniRef50_P75443", "COG:COG1277", "COG:O", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75443", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75443" ], "product": "Uncharacterized protein MPN_335" } }, "id": "APKECPOBEN_729", "locus": "GCA002090215_00702" }, { "type": "cds", "sequence": "contig_1", "start": 398734, "stop": 399081, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTACAACAGTTTAACGTCCGACTGTTGGGTAAGTGTCCTTTAGATTTGGCATCGAGTGAAATGTTTGGTAGTCATAAATTTCGACAAATTCCCGCTAAGGTGTTGCACTATATCCACCAACACAACATTTATCTAAAAACCATTTTGCAAACACTGTTAGATGAACCGCGCATGCAACACTGTTTGCGCGTTGGTCAGTTAGCTAAAACTTTAGCAGTGGCAAATAAACTCGATGGTAAAACGGCTTATACTGCTGGTGCTTACCATGATCTAGCAAAGCAACTACCCCAAGCGCAATTAGAGAAATTAGCCAAAGTAGCGGGTGTAAATGATTACCCTAGCTGA", "aa": "MVQQFNVRLLGKCPLDLASSEMFGSHKFRQIPAKVLHYIHQHNIYLKTILQTLLDEPRMQHCLRVGQLAKTLAVANKLDGKTAYTAGAYHDLAKQLPQAQLEKLAKVAGVNDYPS", "aa_hexdigest": "b0ff642501f2cd9c8351a8382548fdd1", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12909.93339999998, "isoelectric_point": 9.48163928985596 }, "id": "APKECPOBEN_730", "locus": "GCA002090215_00703" }, { "type": "cds", "sequence": "contig_1", "start": 399157, "stop": 399486, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01467.32" ], "nt": "GTGATCTTTGGTGGTGCATTTGATCCCCTCCACCAAGCGCATATCTACATTGCTAAGCGTGCTGTACAAGCCATTAAAGCACAAAAGCTTTACTTTGTACCAACTGCTAAAGCCTTTTTTAAATCACCGATTAAAGCATCTAATCAAGCACGTTTAGCAATGTTAAGGGTTGCGCTAAAAGCATTGCCTCAGATGGCAGTATCTAACTTTGACATTAAAGCGCAAAACGGTTTTTCGTTCAATACAGTACAACACTTTAAGCAGCGCTTTCCCAATGCTGAGTTGTACTTTTTAATTGGCAGTGATAAATTAAGCGAGTTGGCCAAATGA", "aa": "MIFGGAFDPLHQAHIYIAKRAVQAIKAQKLYFVPTAKAFFKSPIKASNQARLAMLRVALKALPQMAVSNFDIKAQNGFSFNTVQHFKQRFPNAELYFLIGSDKLSELAK", "aa_hexdigest": "63fda029442a289b48af7ffea810f352", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01467.32", "name": "CTP_transf_like", "length": 106, "aa_cov": 0.963302752293578, "hmm_cov": 0.8773584905660378, "evalue": 2.1102816355208806e-12, "score": 42.86591720581055, "start": 2, "stop": 106 } ], "seq_stats": { "molecular_weight": 12231.238799999988, "isoelectric_point": 10.246040916442869 }, "id": "APKECPOBEN_731", "locus": "GCA002090215_00704" }, { "type": "cds", "sequence": "contig_1", "start": 399571, "stop": 400032, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTAAAAAAGGAACTAGAAATGTACAACCTCTATACTTTTCAAATAGACCTCGATAAAAAGCTTTTATTTGAAAAAGCAGATAATCAACAGAATTACTCTAAGATTAGAGCTCGTTATTTTAAAAATTCTGCACGTAATCAACAAGCAGTTTTTCTCAATAAAAACCTGATTAAAAACACTTTTAATAAGGCACTGTTAAACTTCTCGGATTTTTTGAGTGGTAGTGGTGTAGAGAGCATTTTTAAGCAGGTCATTGATGATCAACCTGAAGTTTTAAACTACCTCAAACAGGTCAAAAAGGAAGATAGTTGCGATGGTCACAGCGAAGCGTCACAGTTAGTATTTAATGTAGTCATTAACCCTAAAAATACGCTCGCTAACTTCTTTGAGGAATTAACCATTTACCTCCACTTTAATGAAGAGAACAACACAGTTGTCGGGAGCTTTTCGTTAAAGTGA", "aa": "MVKKELEMYNLYTFQIDLDKKLLFEKADNQQNYSKIRARYFKNSARNQQAVFLNKNLIKNTFNKALLNFSDFLSGSGVESIFKQVIDDQPEVLNYLKQVKKEDSCDGHSEASQLVFNVVINPKNTLANFFEELTIYLHFNEENNTVVGSFSLK", "aa_hexdigest": "4e64c0d7241bbd2b7fd477021622c291", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17806.978200000005, "isoelectric_point": 6.737716865539551 }, "id": "APKECPOBEN_732", "locus": "GCA002090215_00705" }, { "type": "cds", "sequence": "contig_1", "start": 400228, "stop": 400380, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGAGCAAAAGTACCCTTTTTCCACGATTCACAAAAACTTAAAGATTGTCAATTCTGATTTCTTTGATGCTTTTTTTGTGATCTTGTTGTTAATTTGTCACCTCAATAATAACTTGTTGTGGTTGTGTGAAAAAACTGAGCACTTTGAATGA", "aa": "MEQKYPFSTIHKNLKIVNSDFFDAFFVILLLICHLNNNLLWLCEKTEHFE", "aa_hexdigest": "6526f5c90980bb80098122c6ab14354d", "start_type": "GTG", "rbs_motif": "AAAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6058.011799999996, "isoelectric_point": 5.81379909515381 }, "id": "APKECPOBEN_733", "locus": "GCA002090215_00706" }, { "type": "cds", "sequence": "contig_1", "start": 400441, "stop": 400926, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTGTTGTTTTTAAAGGACTGGTACTTTGAAAACCAAGCCGTTACTAATGAAGACATTGAAAAGGTTGATGAAGTGGAAGACATTGGTAAGTTAGTGGAAAAATACAGCGCTAATCAACCACAAAAGTTGTCTTCTAATTCAACCGTTTATGTATTTGAACCAGACAAAAAACAGTGCTTTTTAAAGAATGATGACTTTTTCAACACTAACGAAGCTAAGCTGTTGTTTTTAATCACAATGCAACCCAATGTTTTTGGTCTGGATGATACTGCCATTGCCAATGACTTAAACCTAAGGGAAATTGGTGACTTCTTTAAGGAAATTGACTTTACTGACAGCGATGTGCTCAATGACTTTCAACAACAAAAAGAAACTTTGTTAGTACGGCGTACCTTTAACCAACTGTTGTTTATGAACAGTAATACCGATGTTTTGAGTATTGTTAACAATAAGTTCAAATCAGCGATCCATAACATTGTCTGA", "aa": "MLLFLKDWYFENQAVTNEDIEKVDEVEDIGKLVEKYSANQPQKLSSNSTVYVFEPDKKQCFLKNDDFFNTNEAKLLFLITMQPNVFGLDDTAIANDLNLREIGDFFKEIDFTDSDVLNDFQQQKETLLVRRTFNQLLFMNSNTDVLSIVNNKFKSAIHNIV", "aa_hexdigest": "1b14b4b571ea121106574fc9cd30a994", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18764.926, "isoelectric_point": 4.459667015075683 }, "id": "APKECPOBEN_734", "locus": "GCA002090215_00707" }, { "type": "cds", "sequence": "contig_1", "start": 400951, "stop": 401268, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTCAAGGCATTTGATTACTCCAAGATCTTTGAGCAAAACCGCACTAACGATCCTTCTCTCTTGCGTTCTAACCTCAACAGCATTAACCGGCTGCGGTATCTCAGTGAATACTTCCGTACCGCTTCAGTTAAGTATGACCAGCTGTACACCAAGGTAAAGGAGTACATGCAATTAGATCAGTTCTTAGTAGACATGATTAACCAGGTCAACCATGAAGATGAGATCTTTGGTAAATACAAGGAACGGGTTTATTTGAGTTTGGGAATAGTTACTGCTGTTGTCTTTGGTATTATTGAGTTCTTTAACTGTGTTTGA", "aa": "MLKAFDYSKIFEQNRTNDPSLLRSNLNSINRLRYLSEYFRTASVKYDQLYTKVKEYMQLDQFLVDMINQVNHEDEIFGKYKERVYLSLGIVTAVVFGIIEFFNCV", "aa_hexdigest": "c5cde1f6fda7d44f9e08dadb56cfb401", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12541.246299999995, "isoelectric_point": 6.566063117980956 }, "id": "APKECPOBEN_735", "locus": "GCA002090215_00708" }, { "type": "cds", "sequence": "contig_1", "start": 401439, "stop": 402419, "strand": "+", "frame": 3, "gene": null, "product": "MPN338 family protein", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P47484", "UniRef:UniRef90_P75440" ], "nt": "ATGGAATTCAATAAGTTACAACTCAGTCACTGTATTTCCTTTTACATTTCAGACGTTTCGGAAGTGTTCTTTGAAAGTATTAACCAACACCCTAGTCGTGATTTTGTCAACAACATCCTGCAAAAGATTAAGAGCACTCTCAGTGAAGAGGAATTGGAAAAGCTCAACACCATTGAGGAAGTTACTAAGGATGAGAAAATTTTGATCATGCTCAACCATGTGTTGAAGAAGATTGTTTCTAAAACGGGTAGTTCTAGCTGTGACTTATTTCAAATAGTGAAACACGACCGCTTTAACGAGCCGGTTTACATCCAAAGTGTCAATGCCTTTGAAAACAATTTGATTAACAATGAATTTGCGGAAAGGCGGTATGACTATTTAATTGAAATTAATAAACACTCTTATTTAAGGAAGTATGTCAATGCCATTCGCATCTCCTTCTTTTTAGATTTAAGGGCGCAAATCTTGTCAGGTTCCTTTACTTTGGATGTGATTAACAAACAGTTGGAACACCAAAATAAGGAAGAGTTGTTCCAAGCGATTTACTTACGCTCCTTAATTAAGCATTTTATCTCGAACCAACTTTACCCGATTTCCTTAAATTCGTTTATTTTTGGTGATAAAAACCGTGAAAACAAGACGGTTGAAAACGACAAGCTTAGTGTGCTTAAAAACAATTGGAATCAAATCTTCTTTTCTAAGTACTTTGATTTTGTTGCCAAAAACAAAGAAGAGCGTGTTGTCGATAACAACTGCGATGAGCTGTTTTACGCCACCATGAACACGCTCTTGATTATGTTGATCATTATTGAAGAGCTCCGGGTATACTTCAACAGTAAAGAGCCAGCCTTAATCTTAAAGCTACTAGATAACAAGGTTTCACTGCGCGAAGACCCCGACCAAAATCCAGAAACTGATTTGCATGAGTTAATTAAGTTTGCTGAAAAGAATTACCTCGAAAAGGAAAAAACAAGTCGCTGA", "aa": "MEFNKLQLSHCISFYISDVSEVFFESINQHPSRDFVNNILQKIKSTLSEEELEKLNTIEEVTKDEKILIMLNHVLKKIVSKTGSSSCDLFQIVKHDRFNEPVYIQSVNAFENNLINNEFAERRYDYLIEINKHSYLRKYVNAIRISFFLDLRAQILSGSFTLDVINKQLEHQNKEELFQAIYLRSLIKHFISNQLYPISLNSFIFGDKNRENKTVENDKLSVLKNNWNQIFFSKYFDFVAKNKEERVVDNNCDELFYATMNTLLIMLIIIEELRVYFNSKEPALILKLLDNKVSLREDPDQNPETDLHELIKFAEKNYLEKEKTSR", "aa_hexdigest": "fbed913fb571d91e2bfae898eeb5a5c4", "start_type": "ATG", "rbs_motif": "AACAA", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 401439, "stop": 402722, "observations": { "mutation": [ 402417 ], "stop": [ 402417 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75440", "query_cov": 1.0, "subject_cov": 0.5158227848101266, "identity": 1.0, "score": 631.0, "evalue": 1.73e-223, "gene_start": 1, "gene_end": 326, "reference_start": 1, "reference_end": 326, "reference_sequence": "MEFNKLQLSHCISFYISDVSEVFFESINQHPSRDFVNNILQKIKSTLSEEELEKLNTIEEVTKDEKILIMLNHVLKKIVSKTGSSSCDLFQIVKHDRFNEPVYIQSVNAFENNLINNEFAERRYDYLIEINKHSYLRKYVNAIRISFFLDLRAQILSGSFTLDVINKQLEHQNKEELFQAIYLRSLIKHFISNQLYPISLNSFIFGDKNRENKTVENDKLSVLKNNWNQIFFSKYFDFVAKNKEERVVDNNCDELFYATMNTLLIMLIIIEELRVYFNSKEPALILKLLDNKVSLREDPDQNPETDLHELIKFAEKNYLEKEKTSRWHKKRVKSLEELLEEIKQINLETKNESLAYPDEIVELELDNVHNFVSTKQVFRHQLDLQTLHGIVINPERYGIGMWSNHFVDWEEFKDLIEQITDAENGSDLYGFEKDLDESICQVNKKYLTFISSDSSSFLIIKNDQTKVISNYVWAQLYFETRRWIINDIEYDLYEKGFDKSHFASNIALLESLNFNWLDPFYGLTSIKEIMQKIDSKSNLKTSIAEMVAKFKHEQRISKKDNERVLMIFAYVAAAVVGFINFFSMVFTILTVSDLNAGLTPANIVVIAIASLLALFLIVIAVLFRFRWKYIKH" }, "paralog": false, "identity": 0.9976580796252927, "score": 845.0, "evalue": 5.38e-310, "description": "Internal stop codon at 402417. Nonsense mutation around 402417.", "psc": { "uniref90_id": "UniRef90_P75440", "product": "MPN338 family protein", "uniref50_id": "UniRef50_P47484", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75440", "UniRef:UniRef50_P47484", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47484", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47484" ], "product": "Uncharacterized protein MG242" } }, "id": "APKECPOBEN_736", "locus": "GCA002090215_00709" }, { "type": "cds", "sequence": "contig_1", "start": 402519, "stop": 402986, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGAATTAGAGTTAGATAACGTTCACAACTTTGTCTCTACTAAACAGGTTTTTCGTCACCAGTTAGACTTACAAACTTTACATGGTATAGTCATCAACCCTGAGCGCTATGGTATTGGCATGTGGAGTAACCACTTTGTCGATTGGGAGGAGTTTAAAGATTTAATTGAACAGATTACTGATGCTGAAAATGGTAGTGATCTCTATGGTTTTGAAAAGGACTTGGACGAGAGCATTTGCCAAGTTAACAAGAAGTACTTAACCTTTATTAGTAGTGACAGTAGCTCTTTCTTAATTATTAAGAATGACCAAACTAAGGTTATCTCTAATTATGTCTGGGCGCAACTGTACTTTGAAACGCGCCGGTGGATTATTAATGATATTGAATACGATCTGTATGAAAAGGGGTTTGACAAGAGCCACTTTGCTAGTAACATTGCCCTTTTAGAGAGCTTGAATTTTAACTGA", "aa": "MELELDNVHNFVSTKQVFRHQLDLQTLHGIVINPERYGIGMWSNHFVDWEEFKDLIEQITDAENGSDLYGFEKDLDESICQVNKKYLTFISSDSSSFLIIKNDQTKVISNYVWAQLYFETRRWIINDIEYDLYEKGFDKSHFASNIALLESLNFN", "aa_hexdigest": "6f14ab6eb370bebc7a0ac39820e35106", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18331.2391, "isoelectric_point": 4.609608268737793 }, "id": "APKECPOBEN_737", "locus": "GCA002090215_00710" }, { "type": "cds", "sequence": "contig_1", "start": 403026, "stop": 403319, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAAAGATTGACAGTAAGAGTAATTTAAAAACATCGATTGCCGAAATGGTCGCTAAGTTTAAACACGAACAGCGCATCTCCAAAAAGGACAATGAGCGGGTGTTGATGATCTTTGCTTATGTAGCCGCTGCGGTAGTTGGTTTTATTAACTTTTTTTCGATGGTGTTTACCATTCTTACAGTCAGTGATCTCAATGCTGGTCTCACCCCCGCTAACATTGTTGTTATTGCAATTGCGAGCTTATTGGCCTTATTCTTAATTGTTATTGCTGTTTTATTTCGGTTTAGGTGA", "aa": "MQKIDSKSNLKTSIAEMVAKFKHEQRISKKDNERVLMIFAYVAAAVVGFINFFSMVFTILTVSDLNAGLTPANIVVIAIASLLALFLIVIAVLFRFR", "aa_hexdigest": "939c945166e706743f54903491bbe67b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10793.860399999985, "isoelectric_point": 9.991777610778808 }, "id": "APKECPOBEN_738", "locus": "GCA002090215_00711" }, { "type": "cds", "sequence": "contig_1", "start": 403337, "stop": 403639, "strand": "+", "frame": 2, "gene": null, "product": "putative MG243-like protein", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P75439", "UniRef:UniRef90_P75439" ], "nt": "ATGCAGGTTAAAGTAATTGATGAAAACAGCAATACAACCGCTGTCCTTAACTGCGCTAAAGCGAAAACGCGAGCGCTAGCTTGGTTGTGTGATACAGTACTGTTAGCGATCTTACTCGCAATTATTTACGGCATTAGTAGCATTTTCATCAAAGAACAGTCCAGTGTGTTTTTAATTATGACGGTAAGTCAAGCAGTCTTGTGGTTGACTTACTTTGTCATTTTACCGGGTTTATGGAAGGGCAAAACGCTCTTTCGTGCTTTACTGGGTTTAAGTTTACTCATCTTTAAAAAACGCTTTTGA", "aa": "MQVKVIDENSNTTAVLNCAKAKTRALAWLCDTVLLAILLAIIYGISSIFIKEQSSVFLIMTVSQAVLWLTYFVILPGLWKGKTLFRALLGLSLLIFKKRF", "aa_hexdigest": "133ea20dfc5d0d5ac66f93d9121aa6fb", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 403337, "stop": 403942, "observations": { "mutation": [ 403637, 403667 ], "stop": [ 403637, 403667 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75439", "query_cov": 1.0, "subject_cov": 0.44642857142857145, "identity": 1.0, "score": 186.0, "evalue": 6.22e-58, "gene_start": 1, "gene_end": 100, "reference_start": 1, "reference_end": 100, "reference_sequence": "MQVKVIDENSNTTAVLNCAKAKTRALAWLCDTVLLAILLAIIYGISSIFIKEQSSVFLIMTVSQAVLWLTYFVILPGLWKGKTLFRALLGLSLLIFKKRFWNLLVHELFLWVWYSVIFLALAIYFFVNRDDPKILQAFFDNQNSNLSWIFVKILLSVISVLQLVFVVYFCFSSQKQALQDLLSKSFMVQKAIKVKDCKSELKSTNTIKTHSDLPGDIDLEQLGD" }, "paralog": false, "identity": 0.9900497512437811, "score": 383.0, "evalue": 5.64e-138, "description": "Internal stop codon at 403637, 403667. Nonsense mutation around 403637, 403667.", "psc": { "uniref90_id": "UniRef90_P75439", "product": "Uncharacterized protein MG243 homolog", "uniref50_id": "UniRef50_P75439", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75439", "UniRef:UniRef50_P75439", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75439", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75439" ], "product": "Uncharacterized protein MG243 homolog" } }, "id": "APKECPOBEN_739", "locus": "GCA002090215_00712" }, { "type": "cds", "sequence": "contig_1", "start": 403796, "stop": 404011, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTAAGTGTTATTAGTGTCTTACAACTAGTATTTGTGGTGTATTTCTGTTTCAGCTCCCAAAAGCAAGCGTTGCAAGATTTGTTATCTAAGAGTTTTATGGTGCAAAAAGCAATTAAAGTAAAGGATTGTAAAAGTGAATTGAAGTCCACTAACACTATAAAAACGCACTCTGATTTACCCGGCGACATTGATTTAGAACAATTAGGTGATTAA", "aa": "MLSVISVLQLVFVVYFCFSSQKQALQDLLSKSFMVQKAIKVKDCKSELKSTNTIKTHSDLPGDIDLEQLGD", "aa_hexdigest": "c1129369fbf077abbc59249845ce8ab2", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7951.2137999999995, "isoelectric_point": 6.53377857208252 }, "id": "APKECPOBEN_740", "locus": "GCA002090215_00713" }, { "type": "cds", "sequence": "contig_1", "start": 404015, "stop": 405532, "strand": "+", "frame": 2, "gene": "uvrD", "product": "putative DNA helicase MPN_340", "db_xrefs": [ "COG:COG0210", "COG:L", "EC:5.6.2.4", "GO:0000725", "GO:0003677", "GO:0003689", "GO:0005524", "GO:0005829", "GO:0009378", "GO:0016853", "GO:0016887", "GO:0033202", "GO:0036121", "GO:0061749", "GO:0061775", "GO:0140584", "GO:0140665", "GO:0140849", "GO:1990518", "SO:0001217", "UniRef:UniRef50_P75438", "UniRef:UniRef90_P75438" ], "nt": "ATGGAACATTTAAATCAGGAACAGAAAGCTGCTGTTACTTGTGATAACGGTGTTAACGTGGTGTATTCCGGTGCTGGCACGGGTAAAACAACCGTTATTGCTGAACGCTTTGCTTACTTAGTCAATGAAAAAGGCGTTAATCCTCAAAGTATTTTGGCCTTTACCTTCACTGATAAGGCAGCGAGTGAAATGCGCCAACGCATTATTAAGTTAATTCCACAAAAAAGCTTGCAGGATCTGCACATCTATACCTTTCACAGTTTTGCCAACAGGTTTTTGCAAAAACATGGCAAAAGTGACTTTGCCATCTTGAGCGATTCTAACCGCTTCTTTAGTGATTATGAAATGGGTGATCAACTACAAACAGTGGTGGAAATTTATAAAAACAAAGTAGTTGATTTAGAACTTGACAACCTAGAGTATAACTCCGCTTTTAGAGATGCTTGTACAGATACTTTTAACGAGGATTTTAGTACCATCTCCAATGGTCAGTTTCGTAAACGGGCTGCGACTGCTTTACGGGCTTACCAGAACTATTTAATTACCAATAACCTATTTGATTTTAGCGATTTAATTATTGAAACTTGCCACCTTTTAAAAGGTAATAGTGAATTGTTGCAAGCATTTACCGAAAGTGTGCATTATATCTTAGTAGATGAATTTCAAGACACTAACCTAGCCCAGTACGAATTGGTAAAGTTACTAGCTACAACTCATCCTAATCTCTTTTTGGTGGGTGATAGCAACCAGATGATTTACGGTTGGCGGGGTGCTGTTGTAGAGATCTTTGAACTTTTAAAAAATGATTTTCAAGCAGTCAAAGAGTTTTATACCACCCAAAACTACCGCAGCATCCAAGCGGTGTTAGCAGTAGCGAATGATGTACTAACCGCAATTGCTAGAAAAGAGAGGAAAGCGCTCGTGTTGTTGCACTCTAGCATTGATTCTAAAGCTGTACCGGTACACTATAAGGCCAATTCTTTGAAAAACCAAGACCAGTGGATTATTTACCAAATGAAACAGTTGCATTTAAACAATGGTGTACCTTATGATCAGATGGCGGTGTTATTTCGTAAGAATAAACACCTTGACGCTTTTAGTCAAACAGTGCTGGAAGATGGTGATTTACCTTTAGCTAAATTAAACCTTTTAACAATTCACGCTGCCAAGGGTTTGGAATTTGAGGCAGTATTTGTTTATGGCTTGGTTGAACGCGCTTTTCCTAGTTTGCACTGGGATGGAAGTGATAAGCACAAGTTACTAGAAGAAATGAAACTGTTTTACGTAGCGATTACTCGTGCTAAACAGTTTTTATTTTTAGTTTCTGTTAGTGTGGAAAGTTTCAATGCGTATTATGAGCCGTCACGCTTTTTAAAACTAATTGAAAAGGAACATTTGCAAACACAAAAGGCAGCGTACTTTAAAGAACAATTAAAGACACAGCCAAAACCAGTTAATTTGTATACAGAGACAGAAAACGCGGAAAACTTACAAAAAGCCACTGACTCTAAAAAGTGA", "aa": "MEHLNQEQKAAVTCDNGVNVVYSGAGTGKTTVIAERFAYLVNEKGVNPQSILAFTFTDKAASEMRQRIIKLIPQKSLQDLHIYTFHSFANRFLQKHGKSDFAILSDSNRFFSDYEMGDQLQTVVEIYKNKVVDLELDNLEYNSAFRDACTDTFNEDFSTISNGQFRKRAATALRAYQNYLITNNLFDFSDLIIETCHLLKGNSELLQAFTESVHYILVDEFQDTNLAQYELVKLLATTHPNLFLVGDSNQMIYGWRGAVVEIFELLKNDFQAVKEFYTTQNYRSIQAVLAVANDVLTAIARKERKALVLLHSSIDSKAVPVHYKANSLKNQDQWIIYQMKQLHLNNGVPYDQMAVLFRKNKHLDAFSQTVLEDGDLPLAKLNLLTIHAAKGLEFEAVFVYGLVERAFPSLHWDGSDKHKLLEEMKLFYVAITRAKQFLFLVSVSVESFNAYYEPSRFLKLIEKEHLQTQKAAYFKEQLKTQPKPVNLYTETENAENLQKATDSKK", "aa_hexdigest": "c2d6164d866da3a306582d742c6ce6e5", "start_type": "ATG", "rbs_motif": "AACAA", "psc": { "uniref90_id": "UniRef90_P75438", "query_cov": 1.0, "subject_cov": 0.9546313799621928, "identity": 1.0, "score": 987.0, "evalue": 0.0, "valid": true, "gene": "UvrD", "product": "Probable DNA helicase MPN_340", "ec_ids": [ "5.6.2.4" ], "uniref50_id": "UniRef50_P75438", "cog_id": "COG0210", "cog_category": "L", "go_ids": [ "GO:0005829", "GO:0033202", "GO:0005524", "GO:0016887", "GO:0140849", "GO:0140665", "GO:0140584", "GO:0061775", "GO:0003677", "GO:0003689", "GO:0036121", "GO:0061749", "GO:0009378", "GO:0016853", "GO:1990518", "GO:0000725" ] }, "pscc": { "uniref50_id": "UniRef50_P75438", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75438" ], "product": "Probable DNA helicase MPN_340" }, "genes": [ "uvrD" ], "id": "APKECPOBEN_741", "locus": "GCA002090215_00714" }, { "type": "cds", "sequence": "contig_1", "start": 405667, "stop": 406317, "strand": "+", "frame": 1, "gene": null, "product": "ATP-dependent helicase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0013300ABE", "UniRef:UniRef90_UPI0013300ABE" ], "nt": "ATGGCATTTAACATTAGCACTCAACTCAATAAGGAACAACGAGCCGCTGTTACCTGTGGTAAGGGTGTCAACATTGTCTATTCTGGTGCTGGTACTGGTAAGACCACCATTATTTCCCAACGCTTTGCTTATTTATTTAACCAAAAGCGAATTAATCCTAGCAACATTTTGGCGTTAACCTATACAAGGAAAGCGGCGAGTGAGATGAAGCAGCGGATTTTGGAGCTGTTACCGGAAAAGTATCATAAGGATGTCAACATTTACACCTTCCACAGCTTTTGTAGCCGTTTTTTACGTGAAGAAGGGCAAAAAAACTTTGTGATTGATGATGACTTAAGTAACTTTCTAAAAGACTTTCTCAAAGAGAGTGAACTAAAAAGTCAAAAGGTATTACAAATTATTGACGGTTTTAAAAACGCTTACTTTGATTTTGATACCAACAGCTTAAAGGATGATGAAAGATTGGTCGAGTTGTGTGAGTTTCATTTAGACCCCCAAGAGCGTAACTTTCAACTTTTCAAAGAAACCGCTATTGCTGCTTTTGTTGAATATGAAAAAGGTAAACAACAGAACAATAAAATTGACTTTGCTGATTTATTAATTAAAACTTGCACTTTACTGAGCAAAGATCACAAATTATTGCGCAAGTGA", "aa": "MAFNISTQLNKEQRAAVTCGKGVNIVYSGAGTGKTTIISQRFAYLFNQKRINPSNILALTYTRKAASEMKQRILELLPEKYHKDVNIYTFHSFCSRFLREEGQKNFVIDDDLSNFLKDFLKESELKSQKVLQIIDGFKNAYFDFDTNSLKDDERLVELCEFHLDPQERNFQLFKETAIAAFVEYEKGKQQNNKIDFADLLIKTCTLLSKDHKLLRK", "aa_hexdigest": "d196bf69d5447ba638200a87db8c29ec", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 405667, "stop": 406620, "observations": { "mutation": [ 406315, 406453 ], "stop": [ 406315, 406453 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0013300ABE", "query_cov": 1.0, "subject_cov": 0.494279176201373, "identity": 1.0, "score": 429.0, "evalue": 7.38e-149, "gene_start": 1, "gene_end": 216, "reference_start": 1, "reference_end": 216, "reference_sequence": "MAFNISTQLNKEQRAAVTCGKGVNIVYSGAGTGKTTIISQRFAYLFNQKRINPSNILALTYTRKAASEMKQRILELLPEKYHKDVNIYTFHSFCSRFLREEGQKNFVIDDDLSNFLKDFLKESELKSQKVLQIIDGFKNAYFDFDTNSLKDDERLVELCEFHLDPQERNFQLFKETAIAAFVEYEKGKQQNNKIDFADLLIKTCTLLSKDHKLLRKWSKKFQYILGDEFQDTNQIQYELIKMLASHHQNLFLVGDNNQMIYRWRGAVSDIIDSLKSDFKVRPENEFYITQNYRCDQNILTVANSILGTIYAKENQPDSTKGFLFSAIKSNRLPVYFQASSVEGQHSWIINKIKNLHKNHGIQYKDMAILFRTNRNMDSMTEALEADGSIPLKQNKGFFKQLETFKKVLVALITRSNYDIKLALKSLRVWTNVLNKSH" }, "paralog": false, "identity": 0.9936908517350158, "score": 627.0, "evalue": 5.25e-229, "description": "Internal stop codon at 406315, 406453. Nonsense mutation around 406315, 406453.", "psc": { "uniref90_id": "UniRef90_UPI0013300ABE", "product": "ATP-dependent helicase", "uniref50_id": "UniRef50_UPI0013300ABE", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0013300ABE", "UniRef:UniRef50_UPI0013300ABE" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0013300ABE", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0013300ABE" ], "product": "ATP-dependent helicase" } }, "id": "APKECPOBEN_742", "locus": "GCA002090215_00715" }, { "type": "cds", "sequence": "contig_1", "start": 406762, "stop": 406953, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTATTTTGTTTCGCACCAACAGAAACATGGATTCGATGACTGAAGCGCTTGAAGCTGATGGCAGTATTCCCCTTAAGCAAAATAAGGGTTTTTTTAAACAGTTAGAGACTTTTAAAAAGGTATTAGTGGCACTCATAACTAGGTCAAACTATGACATTAAACTAGCTTTAAAAAGTTTGCGGGTTTGA", "aa": "MAILFRTNRNMDSMTEALEADGSIPLKQNKGFFKQLETFKKVLVALITRSNYDIKLALKSLRV", "aa_hexdigest": "eca3d2b96738a907cdf33187523e4ba2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7218.467499999999, "isoelectric_point": 9.991777610778808 }, "id": "APKECPOBEN_743", "locus": "GCA002090215_00716" }, { "type": "cds", "sequence": "contig_1", "start": 406993, "stop": 407772, "strand": "+", "frame": 1, "gene": null, "product": "ATP-dependent helicase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0013310682", "UniRef:UniRef90_UPI0013310682" ], "nt": "GTGAATTTAGATAAAATCTTGCAAAACTTAGAGCAAGCCATTTTTTTAGATGAGCATACTCGGGGTGAGTTGACGGAAGCTGCTAAAGTTTTTACGCGTTTAATTAAGTTTGTTGAAGAACAACAGTTTGAAGCTTTATTAGCCTTTACTTTTGAGGCATTAAATACTGATCAGTTTGTCTGCAACTTTATTTTTAGAACATTGCAAAAACTGCAAACTGAAAATAAAAACTTCACCATTACCGATTTTATTAACGAGTTAAGGTTCCAACAAGATGAGTTAAAGCAGTCCAAAGATAATGTGATTAATTTAATTACAGTGCACTCTGCTAAGGGCTTGGAATTTGAAGCTGTCTTTATTTATGGGATCAATCAAGACAACTTTCCGCTCAAATCGAAGAATCAAATAATAGATCTACAAAGAGAATTGGATGAACTACGCCTTTTTTATGTGGCCTTGACAAGGGCAAAGAAATACTTGTTTTTACTTTCAGTTTATCAAATGAGTGGTGAAATTATTTACCCCTCTAAATTCATTCGTTTTATCAATAAAGAGGATAGACTAGAAATTGCCACCATTAACCATAAAGTTCACCAGGATGATGAATTCTTCGATTCTTCTAAAACAGAAGACTATCAAAAAAAGTATTTAACAGAAAATACGGATTTTGTTAATGGTGACTCTGTTAGCCACCGTACTTATGGTAAGGGCGTTGTTGTTGAAGTTAGAGAAGATGCAGTCTGTGTCGCTTTTAAAAACAGTAAGTACGGACAGAGGTGA", "aa": "MNLDKILQNLEQAIFLDEHTRGELTEAAKVFTRLIKFVEEQQFEALLAFTFEALNTDQFVCNFIFRTLQKLQTENKNFTITDFINELRFQQDELKQSKDNVINLITVHSAKGLEFEAVFIYGINQDNFPLKSKNQIIDLQRELDELRLFYVALTRAKKYLFLLSVYQMSGEIIYPSKFIRFINKEDRLEIATINHKVHQDDEFFDSSKTEDYQKKYLTENTDFVNGDSVSHRTYGKGVVVEVREDAVCVAFKNSKYGQR", "aa_hexdigest": "243e4dd0ac70085592bd8a9bfcd458a3", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 406813, "stop": 407814, "observations": { "mutation": [ 406951, 407770 ], "stop": [ 406951, 407770 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0013310682", "query_cov": 1.0, "subject_cov": 0.7777777777777778, "identity": 0.996, "score": 501.0, "evalue": 5.51e-178, "gene_start": 1, "gene_end": 259, "reference_start": 61, "reference_end": 319, "reference_sequence": "LEADCSIPLKQNKGFFKQLETFKKVLVALITRSNYDIKLALKSLRVWPNVLNKSLTVNEQVNLDKILQNLEQAIFLDEHTRGELTEAAKVFTRLIKFVEEQQFEALLAFTFEALNTDQFVCNFIFRTLQKLQTENKNFTITDFINELRFQQDELKQSKDNVINLITVHSAKGLEFEAVFIYGINQDNFPLKSKNQIIDLQRELDELRLFYVALTRAKKYLFLLSVYQMSGEIIYPSKFIRFINKEDRLEIATINHKVHQDDEFFDSSKTEDYQKKYLTENTDFVNGDSVSHRTYGKGVVVEVREDAVCVAFKNSKYGQRWIVKNHRDLVKAVY" }, "paralog": false, "identity": 0.990990990990991, "score": 642.0, "evalue": 6.92e-236, "description": "Internal stop codon at 406951, 407770. Nonsense mutation around 406951, 407770.", "psc": { "uniref90_id": "UniRef90_UPI0013310682", "product": "ATP-dependent helicase", "uniref50_id": "UniRef50_UPI0013310682", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0013310682", "UniRef:UniRef50_UPI0013310682" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0013310682", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0013310682" ], "product": "ATP-dependent helicase" } }, "id": "APKECPOBEN_744", "locus": "GCA002090215_00717" }, { "type": "cds", "sequence": "contig_1", "start": 408303, "stop": 408935, "strand": "+", "frame": 3, "gene": null, "product": "site-specific DNA-methyltransferase (adenine-specific)", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_N2BQM8" ], "nt": "TTGCGCGATATCTTCGATGATATTGAAAAGTCTACTGAACTAGGTGATGGAAGAAGTAAAGAATCCTTTAAAGGTCTTTTCAAAGACTTTAATGTTTCTGAGGTTAAGTTAGGTTCTACTTTAACGATCCGAACAGAGAAATTAAAAGAACTTTTAACAAGCATTGACACAATGGAGCTCGATGAATTTGAGAAAAATTCTATCGATGCATTTGGTGATGCATATGAGTTTTTAATTTCCATGTATGCACAAAATGCTGGAAAAAGTGGGGGTGAGTTTTTTACACCCCAAGATATATCTGAACTTCTCGCTAGAATTGCGATTGGCAAAAAGGACACCGTTGATGATGTTTATGACATGGCTTGTGGCAGTGGTTCTTTACTTTTACAAGTAATTAAAGTTTTAGGTAAAGAAAAAACTTCACTGGTTAGCTATTACGGTCAGGAAATAAACCACACAACTTACAACCTTTGTCGGATGAATATGATTCTCCACAATATTGATTACGCTAATTTCAATATTATTAACGCTGATACTTTAACAACGAAGGAGTGGGAGAAGCATTACGTAAATTGCTCAAATGAAAATGGGTTTGAAGTTGTGGTTTCCAATCCACCATATTCCATTAGTTGA", "aa": "MRDIFDDIEKSTELGDGRSKESFKGLFKDFNVSEVKLGSTLTIRTEKLKELLTSIDTMELDEFEKNSIDAFGDAYEFLISMYAQNAGKSGGEFFTPQDISELLARIAIGKKDTVDDVYDMACGSGSLLLQVIKVLGKEKTSLVSYYGQEINHTTYNLCRMNMILHNIDYANFNIINADTLTTKEWEKHYVNCSNENGFEVVVSNPPYSIS", "aa_hexdigest": "2b5d33321406ee3a793245a612dc1ed1", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_N2BQM8", "query_cov": 0.8857142857142857, "subject_cov": 0.9946524064171123, "identity": 0.5379999999999999, "score": 176.0, "evalue": 1.68e-52, "valid": false, "product": "type I restriction-modification system subunit M", "ec_ids": [ "2.1.1.72" ], "uniref50_id": "UniRef50_N2BQM8", "kegg_orthology_id": "K03427", "go_ids": [ "GO:0003677", "GO:0009007", "GO:0009307" ] }, "pscc": { "uniref50_id": "UniRef50_N2BQM8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_N2BQM8" ], "product": "site-specific DNA-methyltransferase (adenine-specific)" }, "genes": [], "id": "APKECPOBEN_745", "locus": "GCA002090215_00718" }, { "type": "cds", "sequence": "contig_1", "start": 408993, "stop": 409364, "strand": "+", "frame": 3, "gene": null, "product": "site-specific DNA-methyltransferase (adenine-specific)", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A376YJJ9" ], "nt": "TTGGCACCTAATTCAAAAGCTGATTTAGCTTTTGTTTTACATGCTCTATATGTATTAGGTCAAGAAGGTACAGCAGCAATTGTTTGTTTCCCTGGAATCTTGTACCGTGAAGGTAAAGAGCAAACCATTAGAAAATACCTAGTTGATCAAAACTTTGTTGATGCAGTGATCCAATTACCAAGTAATCTTTTTAGCACAACATCAATCGCTACTTCAATTCTTGTTCTCAAGAAAAATCGTGATAAAAAAGATCCCATTTTCTTCATTGATGGTTCTAACGAATTTGTTAGAGAAAAGAAGAATAACCGCTTAAGTCCAAAAAATATTGAAAAGATTGTTGATTGCTTTAATAGCAAAAAAAGAAGAAGCTAA", "aa": "MAPNSKADLAFVLHALYVLGQEGTAAIVCFPGILYREGKEQTIRKYLVDQNFVDAVIQLPSNLFSTTSIATSILVLKKNRDKKDPIFFIDGSNEFVREKKNNRLSPKNIEKIVDCFNSKKRRS", "aa_hexdigest": "7a5b939fcb3db43cac950d324b15ad29", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0013DFE473", "query_cov": 0.983739837398374, "subject_cov": 0.8581560283687943, "identity": 0.521, "score": 115.0, "evalue": 7.63e-31, "valid": false, "product": "N-6 DNA methylase", "ec_ids": [ "2.1.1.72" ], "uniref50_id": "UniRef50_A0A376YJJ9", "kegg_orthology_id": "K03427" }, "pscc": { "uniref50_id": "UniRef50_A0A376YJJ9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A376YJJ9" ], "product": "site-specific DNA-methyltransferase (adenine-specific)" }, "genes": [], "id": "APKECPOBEN_746", "locus": "GCA002090215_00719" }, { "type": "cds", "sequence": "contig_1", "start": 409330, "stop": 409587, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTGCTTTAATAGCAAAAAAAGAAGAAGCTAATTTTGCAAAGTCGGTTGAAAGGGACAAAATTAGGGAATCTAACTACGACCTTACAGTAGGTAAATACGTAAACTCAGAGGCTGAAAAGGAAGAATTAGATATTAAGGTTTTGAATCACTCTATTGATGAAATAGTAGATAAACAAAAAGATCTAAGAACTAAAATTAAGGATATTATTCAGGATATCAAAGTAGATTTTGACAACATTGATATTAATAACTAG", "aa": "MIALIAKKEEANFAKSVERDKIRESNYDLTVGKYVNSEAEKEELDIKVLNHSIDEIVDKQKDLRTKIKDIIQDIKVDFDNIDINN", "aa_hexdigest": "e03ca84f471a38a0d544c588cc9bc429", "start_type": "TTG", "rbs_motif": "AAAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9878.05629999999, "isoelectric_point": 4.855266380310058 }, "id": "APKECPOBEN_747", "locus": "GCA002090215_00720" }, { "type": "cds", "sequence": "contig_1", "start": 409657, "stop": 409836, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01420.25" ], "nt": "GTGCAAATTAAAACTTACAAAATCAAGGATATTTGCGAGATCTCAAGAGGCAAAGCAATTACTAAAAAATACATTAAGGATAATCCTGGTCAATATCCGGTTTATTCATCTACTACTGCTAATAACGGTGAAATTGGCAGAATAAAGGATTACGATCTTGATGGCGAATACGTTACCTGA", "aa": "MQIKTYKIKDICEISRGKAITKKYIKDNPGQYPVYSSTTANNGEIGRIKDYDLDGEYVT", "aa_hexdigest": "ab2463771057210eb59fcb3706209e77", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01420.25", "name": "Methylase_S", "length": 60, "aa_cov": 0.9491525423728814, "hmm_cov": 0.9833333333333333, "evalue": 7.319335901459064e-12, "score": 41.26675796508789, "start": 4, "stop": 59 } ], "seq_stats": { "molecular_weight": 6735.562900000004, "isoelectric_point": 8.708276557922364 }, "id": "APKECPOBEN_748", "locus": "GCA002090215_00721" }, { "type": "cds", "sequence": "contig_1", "start": 409966, "stop": 410511, "strand": "+", "frame": 1, "gene": "hsdS", "product": "Type I restriction enzyme MpnII specificity subunit", "db_xrefs": [ "COG:COG0732", "COG:V", "EC:3.1.21.3", "GO:0003677", "GO:0009307", "KEGG:K01154", "SO:0001217", "UniRef:UniRef50_P75435", "UniRef:UniRef90_P75435" ], "nt": "ATGGAGGCTCCTAAATTTGTTAATAATGCTTGTCCTATTCCTAACCTCAATTTATCAAGAACTGAGGAAATTGAATTAGATTTCCCACCCTTGCAAATTCAGCAGAAAATTGCTACTATCTTAGATACTTTTACCGAACTAAGCGCCGAACTAAGCGCCGAACTAAGCGCCGAACTAAGCGCCGAACTAAGCGCCGAACTAAGCGCCGAACTAAGCGCCGAACTAAGCGCCGAACTAAGCGCCGAACTAAGCGCCGAACTAAGCGCCGAACTAAGCGCCGAGCTAAGAGAGCGAAAGAAGCAGTATGCCTTCTATCGCGATTATCTGCTCAACCAAGAAAATATCCGAAAAATTTACGGAGCAAATATACCTTTCGAAACTTTTCAAGTAAAGGATATTTGTGAAATTAGAAGAGGTCGAGCGATAACTAAAGCGTATATTAGGAATAATCCTGGAGAAAATCCAGTTTATTCCGCAGCTACCACAAATGATGGTGAGTTAGGACGTATTAAAGATTGCGATTTTGATGGTGAATACATCACTTGA", "aa": "MEAPKFVNNACPIPNLNLSRTEEIELDFPPLQIQQKIATILDTFTELSAELSAELSAELSAELSAELSAELSAELSAELSAELSAELSAELSAELRERKKQYAFYRDYLLNQENIRKIYGANIPFETFQVKDICEIRRGRAITKAYIRNNPGENPVYSAATTNDGELGRIKDCDFDGEYIT", "aa_hexdigest": "e8b5a06b385ccaa9c7fd92f0515a97d3", "start_type": "ATG", "rbs_motif": null, "genes": [ "hsdS" ], "truncated": "both", "pseudogene": { "start": 409666, "stop": 410814, "observations": { "mutation": [ 409834, 410509 ], "stop": [ 409834, 410509 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75435", "query_cov": 1.1767955801104972, "subject_cov": 0.47438752783964366, "identity": 0.85, "score": 242.0, "evalue": 1.27e-75, "gene_start": 1, "gene_end": 181, "reference_start": 104, "reference_end": 316, "reference_sequence": "MQIKTYKIKDICEISRGKAITKKYIKDNPGQYPVYSSTTANNGEIGRIKDYDLDGEYVTWTTDGIYAGTVFYRNEKFNASQHCGVLKLKNNEISAKFLTYALGMEAPKFVNNACPIPNLNLSRTEEIELDFPPLQIQQKIATILDTFTELSAELSAELSAELSAELSAELSAELSAELSAELSAELSAELSAELSAELSAELSAELSAELSAELSAELSAELSAELSAELRERKKQYAFYRDYLLNQENIRKIYGANIPFETFQVKDICEIRRGRAITKAYIRNNPGENPVYSAATTNDGELGRIKDCDFDGEYITWTTNGYAGVVFYRNDKFNASQDCGVLKVKNKKICTKFLSFLLKIEAPKFVHNLASRPKLSQKVMAEIELSFPPLEIQEKIADILFAFEKLCNDLVEGIPAEIELRKKQLDYYQNFLFNWVQEQKKNSLSTNLN" }, "paralog": false, "identity": 0.9154589371980676, "score": 635.0, "evalue": 1.31e-232, "description": "Internal stop codon at 409834, 410509. Nonsense mutation around 409834, 410509.", "psc": { "uniref90_id": "UniRef90_P75435", "gene": "HsdS", "product": "Type I restriction enzyme MpnII specificity subunit", "ec_ids": [ "3.1.21.3" ], "uniref50_id": "UniRef50_P75435", "cog_id": "COG0732", "cog_category": "V", "kegg_orthology_id": "K01154", "go_ids": [ "GO:0003677", "GO:0009307" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75435", "EC:3.1.21.3", "UniRef:UniRef50_P75435", "COG:COG0732", "COG:V", "KEGG:K01154", "GO:0003677", "GO:0009307" ] }, "pscc": { "uniref50_id": "UniRef50_P75435", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75435" ], "product": "Type I restriction enzyme MpnII specificity subunit" } }, "id": "APKECPOBEN_749", "locus": "GCA002090215_00722" }, { "type": "cds", "sequence": "contig_1", "start": 410698, "stop": 410865, "strand": "+", "frame": 1, "gene": null, "product": "restriction endonuclease subunit S", "db_xrefs": [ "EC:3.1.21.3", "KEGG:K01154", "SO:0001217", "UniRef:UniRef50_UPI00190F3D87", "UniRef:UniRef90_UPI00190F3D87" ], "nt": "ATGGCTGAAATTGAACTCTCTTTTCCACCTCTAGAAATTCAAGAAAAGATTGCTGATATTCTCTTTGCCTTTGAAAAACTTTGCAATGATTTAGTAGAAGGTATTCCTGCCGAAATTGAATTGCGTAAAAAGCAATTGGATTACTATCAGAATTTCTTATTTAATTGA", "aa": "MAEIELSFPPLEIQEKIADILFAFEKLCNDLVEGIPAEIELRKKQLDYYQNFLFN", "aa_hexdigest": "bbbc1991845ad67ac2a240d1c026608d", "start_type": "ATG", "rbs_motif": "AAAA", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 410551, "stop": 410910, "observations": { "mutation": [ 410863 ], "stop": [ 410863 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00190F3D87", "query_cov": 1.0, "subject_cov": 0.46218487394957986, "identity": 1.0, "score": 114.0, "evalue": 1.06e-31, "gene_start": 1, "gene_end": 55, "reference_start": 50, "reference_end": 104, "reference_sequence": "GKCNASQDCGVLKVKNKKICTKFLSFLLKIEAPKFVHNLASRPKLSQKVMAEIELSFPPLEIQEKIADILFAFEKLCNDLVEGIPAEIELRKKQLDYYQNFLFNWVQEQKKNSLSTNLN" }, "paralog": false, "identity": 0.9831932773109243, "score": 231.0, "evalue": 4.77e-80, "description": "Internal stop codon at 410863. Nonsense mutation around 410863.", "psc": { "uniref90_id": "UniRef90_UPI00190F3D87", "product": "restriction endonuclease subunit S", "ec_ids": [ "3.1.21.3" ], "uniref50_id": "UniRef50_UPI00190F3D87", "kegg_orthology_id": "K01154", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00190F3D87", "EC:3.1.21.3", "UniRef:UniRef50_UPI00190F3D87", "KEGG:K01154" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00190F3D87", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00190F3D87" ], "product": "restriction endonuclease subunit S" } }, "id": "APKECPOBEN_750", "locus": "GCA002090215_00723" }, { "type": "cds", "sequence": "contig_1", "start": 411133, "stop": 411783, "strand": "+", "frame": 1, "gene": null, "product": "UPF0134 protein MPN_344", "db_xrefs": [ "RefSeq:WP_010874700.1", "SO:0001217", "UniParc:UPI000013A83F", "UniRef:UniRef100_P75434", "UniRef:UniRef50_P75100", "UniRef:UniRef90_P75434" ], "nt": "ATGCCTAAAATTATTTCTAAACGAAAATTCAACATTCTTAGAAAAACAAAAGAAGAAATCTTTTCTCAAATTGTTTACACTAAGAAGAGAAACGGTCTGCACAAGGCTGCCAAAGAATATTACTTCAAGGACAAAGACTTTACAATAATAGAAGATCAACAGGATCGACCTGATAAACCTGAGGAACTGGACACACCGGATATTCCAAAGCCACCAAAACCTCCCAAGGGTCCTGATCAACCCGAGGAACCAGGCCAACCAGGTGGTCCCGATGATCCTAATTCAGGAAATAAAAAGATGCCAAAGCCAGACGAATTTGTAACCCACAGACAACTTCAAGAATTTAAGAAGGATCTTTTAGTGGAACTTCACGAAATCTTTCCTACAAAACCTGAATTAAAACGGGTTGAAGAAAAAGTTGATGTTCTGTTTGAACTTCAAAAAGCTCAGGGTGAACAAATAAGAATCCAAGGTGAACAAATCAAAGAACTGAAAGTGGAGCAAAAAGCCCAGGGCGAAACCCTACAGCTAATCTTGCAAACACTACAAAAGATGAACGATCGTCTGGATAAGATCGAAGGTAAAATGGACAAAATGGAAAGTAGAATGGATAAAATGGAAACTCGCTTAGACAAACTAGAGTCCAAATAA", "aa": "MPKIISKRKFNILRKTKEEIFSQIVYTKKRNGLHKAAKEYYFKDKDFTIIEDQQDRPDKPEELDTPDIPKPPKPPKGPDQPEEPGQPGGPDDPNSGNKKMPKPDEFVTHRQLQEFKKDLLVELHEIFPTKPELKRVEEKVDVLFELQKAQGEQIRIQGEQIKELKVEQKAQGETLQLILQTLQKMNDRLDKIEGKMDKMESRMDKMETRLDKLESK", "aa_hexdigest": "f0e9f8120bc94c9111532b3756659c85", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013A83F", "ncbi_nrp_id": "WP_010874700.1", "uniref100_id": "UniRef100_P75434", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013A83F", "RefSeq:WP_010874700.1", "UniRef:UniRef100_P75434" ] }, "ips": { "uniref100_id": "UniRef100_P75434", "uniref90_id": "UniRef90_P75434" }, "psc": { "uniref90_id": "UniRef90_P75434", "product": "UPF0134 protein MPN_344", "uniref50_id": "UniRef50_P75100" }, "pscc": { "uniref50_id": "UniRef50_P75100", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75100" ], "product": "UPF0134 protein MPN_013" }, "genes": [], "id": "APKECPOBEN_751", "locus": "GCA002090215_00724" }, { "type": "cds", "sequence": "contig_1", "start": 413019, "stop": 413348, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00270.35" ], "nt": "TTGGCTAGTGAAATAGACTTTGTTGATAAAGTCCTTTTCGTTGTTGACAGGAAAGACTTGGACAACCAAACGATCGAAGAATATGAAAAGTTTCAAGCGGGTTCTGTTAGCGAAACTGAGAACACAAATGATCTTAAAGAAAAGATATTAGACGATAGTACCGCCACACGTGCAATTGTTACTACTATTCATAAACTTAAGAGGTTAATAGATCAGAGAAGTAAGCTTAAAGACGAAGATCTTAAAAAGAAAAACATCGTTCTTATATTCGATGAATGTCATCGATCTCAATTTGGAAAAATGAAACAAGAAATCGATGAGTTTTTTTAA", "aa": "MASEIDFVDKVLFVVDRKDLDNQTIEEYEKFQAGSVSETENTNDLKEKILDDSTATRAIVTTIHKLKRLIDQRSKLKDEDLKKKNIVLIFDECHRSQFGKMKQEIDEFF", "aa_hexdigest": "b623ee7f176d34416f914f4348479b33", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00270.35", "name": "DEAD", "length": 100, "aa_cov": 0.8899082568807339, "hmm_cov": 0.93, "evalue": 2.2561072119222392e-07, "score": 26.185123443603516, "start": 10, "stop": 106 } ], "seq_stats": { "molecular_weight": 12799.364999999987, "isoelectric_point": 5.0787572860717765 }, "id": "APKECPOBEN_752", "locus": "GCA002090215_00725" }, { "type": "cds", "sequence": "contig_1", "start": 413478, "stop": 413825, "strand": "+", "frame": 3, "gene": null, "product": "Putative type I restriction enzyme MpnIIP endonuclease subunit middle part", "db_xrefs": [ "RefSeq:WP_010874702.1", "SO:0001217", "UniParc:UPI000013A844", "UniRef:UniRef100_P75432", "UniRef:UniRef50_P75432", "UniRef:UniRef90_P75432" ], "nt": "ATGTCTGATGCTTTGGATGACGAAAATGTACTTTGTTTTAGGTATAAGAGTTATTACATTAAAGATGATGGAAGCCGAATTGATACATTAAATGAAGAGAGAATTAAGCATATTGTTGATTTCACTTTAGATAAATATCTAGATGCAACACAAAATACTCCGAAATTTGATAATTTTGAAAATCCTGTAAAAGAAGGGTTTAATTCGATTTTTGCCTGTGAGAGTAAAGAAGCAGCAATCGAATACTATAAAGAATTTAAAAAACAAATTGAACAAAAGAAGCTACCAATTAAAATTGCAAGCATTATAGCGCAAACGAAGGTAGTGATGAACAAGAAATTAGTGTAA", "aa": "MSDALDDENVLCFRYKSYYIKDDGSRIDTLNEERIKHIVDFTLDKYLDATQNTPKFDNFENPVKEGFNSIFACESKEAAIEYYKEFKKQIEQKKLPIKIASIIAQTKVVMNKKLV", "aa_hexdigest": "baa01d1bcd6606b7f793ac25aacdf3f2", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013A844", "ncbi_nrp_id": "WP_010874702.1", "uniref100_id": "UniRef100_P75432", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013A844", "RefSeq:WP_010874702.1", "UniRef:UniRef100_P75432" ] }, "ips": { "uniref100_id": "UniRef100_P75432", "uniref90_id": "UniRef90_P75432" }, "psc": { "uniref90_id": "UniRef90_P75432", "product": "Putative type I restriction enzyme MpnIIP endonuclease subunit middle part", "uniref50_id": "UniRef50_P75432" }, "pscc": { "uniref50_id": "UniRef50_P75432", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75432" ], "product": "Putative type I restriction enzyme MpnIIP endonuclease subunit middle part" }, "genes": [], "id": "APKECPOBEN_753", "locus": "GCA002090215_00726" }, { "type": "cds", "sequence": "contig_1", "start": 415208, "stop": 415342, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_014574953.1", "SO:0001217", "UniParc:UPI0001DD653D", "UniRef:UniRef100_A0AB38W8M6", "UniRef:UniRef50_A0AB38W8M6", "UniRef:UniRef90_A0AB38W8M6" ], "nt": "ATGGAAAAGAAAAATAAAAAAAATACCGCAACCGTTCCTACCAAAGAACAATTGGATGGCATGTTTAAATCTCTTGAAAAAGAAGAGAACTATGATGTCTTTGTTCAAGTCATAAAAGCTTTAGGTAAGATGTAG", "aa": "MEKKNKKNTATVPTKEQLDGMFKSLEKEENYDVFVQVIKALGKM", "aa_hexdigest": "9b3c3574738bab55abda7bca2ce6156a", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD653D", "ncbi_nrp_id": "WP_014574953.1", "uniref100_id": "UniRef100_A0AB38W8M6", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD653D", "RefSeq:WP_014574953.1", "UniRef:UniRef100_A0AB38W8M6" ] }, "ips": { "uniref100_id": "UniRef100_A0AB38W8M6", "uniref90_id": "UniRef90_A0AB38W8M6" }, "psc": { "uniref90_id": "UniRef90_A0AB38W8M6", "uniref50_id": "UniRef50_A0AB38W8M6" }, "pscc": { "uniref50_id": "UniRef50_A0AB38W8M6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W8M6" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5089.902800000002, "isoelectric_point": 9.050087928771973 }, "id": "APKECPOBEN_754", "locus": "GCA002090215_00727" }, { "type": "cds", "sequence": "contig_1", "start": 415345, "stop": 415536, "strand": "+", "frame": 1, "gene": null, "product": "Death-on-curing family protein", "db_xrefs": [ "RefSeq:WP_225971189.1", "SO:0001217", "UniParc:UPI000A86DC5E", "UniRef:UniRef100_A0AAX0S2Z8", "UniRef:UniRef50_A0AAX0S2Z8", "UniRef:UniRef90_A0AAX0S2Z8" ], "nt": "GTGCGTGAAATAAACGTTTATTATTTAAGCAACGACTCAAAAAGTGATAAAGACGATATCGATTATTTAATAGAAGAACTTTTAGTTGAAGGTTCTCGTTTTAAAAATAGTCTTGTTACGTTTTTAGAAAAAGAAAAAGACTGCCAATCTTGCATTTGTTTATTTAAATCTAAGCTTTCTTCTTCATTCTAA", "aa": "MREINVYYLSNDSKSDKDDIDYLIEELLVEGSRFKNSLVTFLEKEKDCQSCICLFKSKLSSSF", "aa_hexdigest": "1335dfe0ee308bb53da32da3d4f088e9", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000A86DC5E", "ncbi_nrp_id": "WP_225971189.1", "uniref100_id": "UniRef100_A0AAX0S2Z8", "db_xrefs": [ "SO:0001217", "UniParc:UPI000A86DC5E", "RefSeq:WP_225971189.1", "UniRef:UniRef100_A0AAX0S2Z8" ] }, "ips": { "uniref100_id": "UniRef100_A0AAX0S2Z8", "uniref90_id": "UniRef90_A0AAX0S2Z8" }, "psc": { "uniref90_id": "UniRef90_A0AAX0S2Z8", "product": "Death-on-curing family protein", "uniref50_id": "UniRef50_A0AAX0S2Z8" }, "pscc": { "uniref50_id": "UniRef50_A0AAX0S2Z8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAX0S2Z8" ], "product": "Death-on-curing family protein" }, "genes": [], "id": "APKECPOBEN_755", "locus": "GCA002090215_00728" }, { "type": "cds", "sequence": "contig_1", "start": 415942, "stop": 416061, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATCAAAAACTTTGTAGTTAGTTTTGAAGAACATAAAAGAGAAAACAGGGATGAATTTCTAGCAAAGCAAACAAAGAAATTTATCATGGACAACATCCTTTTAGCTCTCAATTTTTGA", "aa": "MIKNFVVSFEEHKRENRDEFLAKQTKKFIMDNILLALNF", "aa_hexdigest": "2f3b8c462bd921c9950515ac9f9d514c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4758.521099999999, "isoelectric_point": 8.162164878845214 }, "id": "APKECPOBEN_756", "locus": "GCA002090215_00729" }, { "type": "cds", "sequence": "contig_1", "start": 416117, "stop": 416611, "strand": "+", "frame": 2, "gene": "fAU1", "product": "5-formyltetrahydrofolate cyclo-ligase", "db_xrefs": [ "COG:COG0212", "COG:H", "EC:6.3.3.2", "GO:0005524", "GO:0005737", "GO:0006730", "GO:0009396", "GO:0030272", "GO:0035999", "GO:0046872", "RefSeq:WP_010874704.1", "SO:0001217", "UniParc:UPI00001394EC", "UniRef:UniRef100_P75430", "UniRef:UniRef50_P75430", "UniRef:UniRef90_P75430" ], "nt": "ATGGACAAAAATGCCTTAAGAAAACAAATTCTGCAAAAAAGAATGGCATTAAGTACCATTGAAAAAAGTCACTTAGATCAAAAGATTAATCAAAAATTAGTTGCTTTTTTAACTCCTAAGCCATGCATTAAAACAATTGCACTTTATGAACCCATTAAAAATGAGGTTACTTTTGTTGACTTCTTCTTTGAGTTTTTAAAGATTAACCAAATAAGAGCTGTTTACCCCAAGGTAATAAGTGATACCGAAATTATCTTTATTGATCAGGAGACAAATACATTCGAACCTAATCAAATAGACTGCTTCCTAATACCATTGGTAGGCTTTAATAAAGACAATTACCGTCTAGGCTTTGGCAAGGGCTATTATGACCGTTATTTAATGCAATTAACTAGACAACAACCTAAAATAGGAATAGCGTACAGTTTCCAAAAAGGTGATTTTTTAGCCGATCCTTGGGATGTACAACTAGACTTAATTATTAATGATGAATAG", "aa": "MDKNALRKQILQKRMALSTIEKSHLDQKINQKLVAFLTPKPCIKTIALYEPIKNEVTFVDFFFEFLKINQIRAVYPKVISDTEIIFIDQETNTFEPNQIDCFLIPLVGFNKDNYRLGFGKGYYDRYLMQLTRQQPKIGIAYSFQKGDFLADPWDVQLDLIINDE", "aa_hexdigest": "a9956fc88c9aea3f4484912cbd983518", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001394EC", "ncbi_nrp_id": "WP_010874704.1", "uniref100_id": "UniRef100_P75430", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001394EC", "RefSeq:WP_010874704.1", "UniRef:UniRef100_P75430" ] }, "ips": { "uniref100_id": "UniRef100_P75430", "uniref90_id": "UniRef90_P75430" }, "psc": { "uniref90_id": "UniRef90_P75430", "gene": "FAU1", "product": "5-formyltetrahydrofolate cyclo-ligase", "ec_ids": [ "6.3.3.2" ], "uniref50_id": "UniRef50_P75430", "cog_id": "COG0212", "cog_category": "H", "go_ids": [ "GO:0005524", "GO:0005737", "GO:0006730", "GO:0009396", "GO:0030272", "GO:0035999", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_P75430", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75430" ], "product": "5-formyltetrahydrofolate cyclo-ligase" }, "genes": [ "fAU1" ], "id": "APKECPOBEN_757", "locus": "GCA002090215_00730" }, { "type": "cds", "sequence": "contig_1", "start": 416601, "stop": 417446, "strand": "+", "frame": 3, "gene": "ymdB", "product": "Putative phosphatase/phosphodiesterase MPN_349", "db_xrefs": [ "COG:COG1692", "COG:T", "EC:3.1.-.-", "GO:0004113", "GO:0046872", "KEGG:K09769", "RefSeq:WP_010874705.1", "SO:0001217", "UniParc:UPI00001394F4", "UniRef:UniRef100_P75429", "UniRef:UniRef50_P75429", "UniRef:UniRef90_P75429" ], "nt": "ATGATGAATAGCATTAAATTCATTTTCCTAGGTGACGTCTATGGCAAGGCCGGACGTAATATTATTAAGAATAATCTAGCCCAACTCAAATCAAAGTATCAAGCTGATTTAGTCATTGTTAATGCCGAAAATACGACTCACGGTAAAGGACTTAGTTTAAAGCACTATGAGTTTCTAAAAGAGGCAGGCGTTAATTACATTACTATGGGTAATCATACCTGGTTTCAGAAGCTAGATTTAGCTGTTGTAATTAATAAAAAGGACTTAGTGCGTCCTTTAAATTTAGACACCAGTTTTGCTTTTCATAACTTAGGGCAAGGTAGTCTTGTATTTGAGTTTAATAAAGCAAAGATTAGGATAACTAATTTACTGGGTACTAGTGTGCCCTTACCCTTTAAAACAACTAATCCCTTTAAAGTTTTAAAAGAACTAATTTTAAAGCGCGATTGTGATCTTCATATAGTTGATTTTCACGCTGAAACCACCAGTGAAAAAAACGCCTTTTGCATGGCTTTTGATGGTTATGTCACAACCATTTTCGGCACCCACACCCACGTGCCTAGTGCTGATTTAAGAATCACCCCCAAAGGTAGTGCTTACATTACCGATGTCGGGATGTGTGGTCCGGGATTTGGTAGTGTTATTGGTGCTAACCCCGAACAGTCGATCAGACTCTTTTGTGCTGGTAGCAGAGAACACTTTGAGGTATCTAAATGCGGAGCACAACTCAATGGTGTCTTCTTTGAGGTTGATGTAAATACAAAAAAGGTGATTAAAACAGAGGCGATTAGGATTGTGGAGGACGATCCTAGATACTTAAAACAAGACTACTTTAACCTAATTTAA", "aa": "MMNSIKFIFLGDVYGKAGRNIIKNNLAQLKSKYQADLVIVNAENTTHGKGLSLKHYEFLKEAGVNYITMGNHTWFQKLDLAVVINKKDLVRPLNLDTSFAFHNLGQGSLVFEFNKAKIRITNLLGTSVPLPFKTTNPFKVLKELILKRDCDLHIVDFHAETTSEKNAFCMAFDGYVTTIFGTHTHVPSADLRITPKGSAYITDVGMCGPGFGSVIGANPEQSIRLFCAGSREHFEVSKCGAQLNGVFFEVDVNTKKVIKTEAIRIVEDDPRYLKQDYFNLI", "aa_hexdigest": "f7efcd5505e4e1a5cd1366968b50b3de", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001394F4", "ncbi_nrp_id": "WP_010874705.1", "uniref100_id": "UniRef100_P75429", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001394F4", "RefSeq:WP_010874705.1", "UniRef:UniRef100_P75429" ] }, "ips": { "uniref100_id": "UniRef100_P75429", "uniref90_id": "UniRef90_P75429" }, "psc": { "uniref90_id": "UniRef90_P75429", "gene": "YmdB", "product": "Putative phosphatase/phosphodiesterase MPN_349", "ec_ids": [ "3.1.-.-" ], "uniref50_id": "UniRef50_P75429", "cog_id": "COG1692", "cog_category": "T", "kegg_orthology_id": "K09769", "go_ids": [ "GO:0004113", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_P75429", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75429" ], "product": "Putative phosphatase/phosphodiesterase MPN_349" }, "genes": [ "ymdB" ], "id": "APKECPOBEN_758", "locus": "GCA002090215_00731" }, { "type": "cds", "sequence": "contig_1", "start": 417640, "stop": 417822, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATCGCTAAAATAAAGAAGGTAACTAAGCTAGCTAAAGAAACGTACTTGGTTATTAAAGTAACTAGCAATCACAAAACTAAGCAAATAACAAATCACCACAGCGAAATTGCCAGCAAAGAACCGCCTGTAGTAGCGATGGCTTTACCACCTTTAAACTTAAAGTACAGTGGAAAAATATGA", "aa": "MIAKIKKVTKLAKETYLVIKVTSNHKTKQITNHHSEIASKEPPVVAMALPPLNLKYSGKI", "aa_hexdigest": "7e73270642d0ba9ca62ab575e34a0813", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6680.9648, "isoelectric_point": 10.06707668304443 }, "id": "APKECPOBEN_759", "locus": "GCA002090215_00732" }, { "type": "cds", "sequence": "contig_1", "start": 417735, "stop": 418157, "strand": "-", "frame": 2, "gene": "plsY", "product": "glycerol-3-phosphate 1-O-acyltransferase PlsY", "db_xrefs": [ "COG:COG0344", "COG:I", "EC:2.3.1.15", "EC:2.3.1.275", "GO:0005886", "GO:0006654", "GO:0008374", "GO:0008654", "GO:0043772", "KEGG:K08591", "SO:0001217", "UniRef:UniRef50_P75428", "UniRef:UniRef90_P75428" ], "nt": "ATGAACGCAGCTAGCGCCATCGCTCTACTTATTGTCTTTAGCCTTGTTATTGGCTATTTAATGGGTTCAGTGATGTTTGCTGATGTTTTTGGCAAGATCTTAAACAAAGATGTCAGGAAATTGGGCTCCAAGAATCCGGGGGCTACTAACTCAATCCGTGTTTTTGGTTTGAAAATAGGCTTCTTAGTTGGTTTATGTGATGCTTTAAAGGGGTTTTTGGCGTTTGTTTTCAGCTTTTTAATCTTTAGCTTTTGGTTACAACAATACCTTAATGTAAACCAGTATCAAAAGGTTTATTACCTCACTTATTTAAGTTGTTTTGCAGCTACCATTGGTCATATTTTTCCACTGTACTTTAAGTTTAAAGGTGGTAAAGCCATCGCTACTACAGGCGGTTCTTTGCTGGCAATTTCGCTGTGGTGA", "aa": "MNAASAIALLIVFSLVIGYLMGSVMFADVFGKILNKDVRKLGSKNPGATNSIRVFGLKIGFLVGLCDALKGFLAFVFSFLIFSFWLQQYLNVNQYQKVYYLTYLSCFAATIGHIFPLYFKFKGGKAIATTGGSLLAISLW", "aa_hexdigest": "0c841b5f00599d8a747333815bfa2b44", "start_type": "ATG", "rbs_motif": "AATAA", "genes": [ "plsY" ], "truncated": "5-prime", "pseudogene": { "start": 417438, "stop": 418157, "observations": { "mutation": [ 417506, 417530, 417713, 417737 ], "stop": [ 417506, 417530, 417713, 417737 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75428", "query_cov": 1.0, "subject_cov": 0.5857740585774058, "identity": 1.0, "score": 270.0, "evalue": 3.75e-90, "gene_start": 1, "gene_end": 140, "reference_start": 1, "reference_end": 140, "reference_sequence": "MNAASAIALLIVFSLVIGYLMGSVMFADVFGKILNKDVRKLGSKNPGATNSIRVFGLKIGFLVGLCDALKGFLAFVFSFLIFSFWLQQYLNVNQYQKVYYLTYLSCFAATIGHIFPLYFKFKGGKAIATTGGSLLAISLWWFVICLVLWLLVTLITKYVSLASLVTFFILAIIILVPWLDYLYFFKPNPINAISYQNDWYIILFFVLWYWPLTIAVFWLHRKNIHRLLNKTENKVTQLN" }, "paralog": false, "identity": 0.9832635983263598, "score": 464.0, "evalue": 6.86e-169, "description": "Internal stop codon at 417506, 417530, 417713, 417737. Nonsense mutation around 417506, 417530, 417713, 417737.", "psc": { "uniref90_id": "UniRef90_P75428", "gene": "plsY", "product": "glycerol-3-phosphate 1-O-acyltransferase PlsY", "ec_ids": [ "2.3.1.15", "2.3.1.275" ], "uniref50_id": "UniRef50_P75428", "cog_id": "COG0344", "cog_category": "I", "kegg_orthology_id": "K08591", "go_ids": [ "GO:0005886", "GO:0006654", "GO:0008374", "GO:0008654", "GO:0043772" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75428", "EC:2.3.1.15", "EC:2.3.1.275", "UniRef:UniRef50_P75428", "COG:COG0344", "COG:I", "KEGG:K08591", "GO:0005886", "GO:0006654", "GO:0008374", "GO:0008654", "GO:0043772" ] }, "pscc": { "uniref50_id": "UniRef50_P75428", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75428" ], "product": "Glycerol-3-phosphate acyltransferase" } }, "id": "APKECPOBEN_760", "locus": "GCA002090215_00733" }, { "type": "cds", "sequence": "contig_1", "start": 418163, "stop": 418354, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGATAAAACAAAGGCGCAAACCCAGTATTTAACTGAAGAACAAATGATCTTGGGTCCGCATTTGGTTAACTTTACTGATAAACAAATGTTAGTGAAATACTACAACGGCTTGCTAAAAAACTTTACAGCACGGTTAAATCCAAGCCAGTTTGATAGTCAAGTAATCCATGTATTAAATAAAATTATTTAA", "aa": "MDKTKAQTQYLTEEQMILGPHLVNFTDKQMLVKYYNGLLKNFTARLNPSQFDSQVIHVLNKII", "aa_hexdigest": "4ba7ab20f856bfe1ca7f121bcb662a24", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7369.4989000000005, "isoelectric_point": 9.100889015197755 }, "id": "APKECPOBEN_761", "locus": "GCA002090215_00734" }, { "type": "cds", "sequence": "contig_1", "start": 418412, "stop": 418804, "strand": "-", "frame": 3, "gene": "trmK", "product": "Putative tRNA methyltransferase MPN_351", "db_xrefs": [ "COG:COG2384", "COG:J", "EC:2.1.1.-", "GO:0005737", "GO:0008033", "GO:0032259", "GO:0160105", "KEGG:K06967", "SO:0001217", "UniRef:UniRef50_P47490", "UniRef:UniRef90_P75427" ], "nt": "ATGAAAAGAAGGATTAGCACAATTGCTAATTTAGTCTTACAGCAAAAACCCAAAGCCTTTTATGACATTGGTTGTGACCATGGCTATTTAGCACGTGAACTAGTTCAACAAAACCCTAGTTTAGTGGGCGTTAACAGTGATATTAGTGCTAATGCATTAGGTGCTGCTAAAGGGTTGTTAAAAGATCACACTAACATGCACTTTATTACCAGTGACGGGTTTGACCTGTTACCTAACTTAAACTTAGATCCTGCTGTTGGTGTTATTGCTGGTTTAGGTGGGTTGAAAATTATGCAAATACTAGCACAGCACAATAACTTTTTAAAGCACTTTGTACTCCAACCGCAGTCCAACATCATTGAATTGCGCCGCTTTTTAAGTGCTAATCAATGA", "aa": "MKRRISTIANLVLQQKPKAFYDIGCDHGYLARELVQQNPSLVGVNSDISANALGAAKGLLKDHTNMHFITSDGFDLLPNLNLDPAVGVIAGLGGLKIMQILAQHNNFLKHFVLQPQSNIIELRRFLSANQ", "aa_hexdigest": "b75d3079488211a97740c68c3bcbca6a", "start_type": "ATG", "rbs_motif": null, "genes": [ "trmK" ], "truncated": "5-prime", "pseudogene": { "start": 418144, "stop": 418804, "observations": { "insertion": [ 418165 ], "mutation": [ 418414 ], "stop": [ 418414 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75427", "query_cov": 1.0, "subject_cov": 0.593607305936073, "identity": 0.992, "score": 256.0, "evalue": 3.84e-85, "gene_start": 1, "gene_end": 130, "reference_start": 1, "reference_end": 130, "reference_sequence": "MKRRISTIANLVLQQKPKAFYDIGCGHGYLARELVQQNPSLVGVNSDISANALGAAKGLLKDHTNMHFITSDGFDLLPNLNLDPAVGVIAGLGGLKIMQILAQHNNFLKHFVLQPQSNIIELRRFLSANQWTITTETLVEERGFIYLVLAVDKTKAQTQYLTEEQMILGPHLVNFTDKQMLVKYYNGLLKNFTARLNPSQFDSQVIHVLNKIIKAYERS" }, "paralog": false, "identity": 0.9908675799086758, "score": 421.0, "evalue": 2.55e-152, "description": "Internal stop codon at 418414. Frameshift due to insertion around 418165. Nonsense mutation around 418414.", "psc": { "uniref90_id": "UniRef90_P75427", "gene": "TrmK", "product": "Putative tRNA methyltransferase MPN_351", "ec_ids": [ "2.1.1.-" ], "uniref50_id": "UniRef50_P47490", "cog_id": "COG2384", "cog_category": "J", "kegg_orthology_id": "K06967", "go_ids": [ "GO:0005737", "GO:0160105", "GO:0032259", "GO:0008033" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75427", "EC:2.1.1.-", "UniRef:UniRef50_P47490", "COG:COG2384", "COG:J", "KEGG:K06967", "GO:0005737", "GO:0160105", "GO:0032259", "GO:0008033" ] }, "pscc": { "uniref50_id": "UniRef50_P47490", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47490" ], "product": "Putative tRNA methyltransferase MG248" } }, "id": "APKECPOBEN_762", "locus": "GCA002090215_00735" }, { "type": "cds", "sequence": "contig_1", "start": 418788, "stop": 420287, "strand": "-", "frame": 2, "gene": "sigA", "product": "RNA polymerase sigma factor", "db_xrefs": [ "COG:COG0568", "COG:K", "GO:0003677", "GO:0005737", "GO:0006352", "GO:0016987", "RefSeq:WP_010874708.1", "SO:0001217", "UniParc:UPI0000134830", "UniRef:UniRef100_P78022", "UniRef:UniRef50_P78022", "UniRef:UniRef90_P78022" ], "nt": "ATGTCATCGCCAAAGAAAAATTTCAAAAAACCTCAGCCTAAAACTGAAAATCAAAAACAAGCACTTAACGAAGAGCGCATTGCTGAACTTAAAAAGAGTCGTATCCTCGGTAAAAACCGTCCGTTTAAAAAGATGATTTACGTTGACACTAAGGCACAACGTAAACAAAAACACGAAAACGTAGCCTTTTTAAAAACGCTTCAAGAAAACAAGGAGAGTGATGTACCAAAGAAGCGTCGCGGTCGAAAGCCAAAACACGCACCTTTAAAGGAAAAGAACAACCTTAAGCTGTTTGATATTTTAGAAGGCTCTCTAAAGAGTCATACCGAAAATGATGATACTAACAAGGTAATTAGCCTGTTGGTGGAGGTGTGGGAGAAAAAGAACAAAAAGAAAGACAATAACTCGCTTTCCAACAAGGACATTGTTAATGTTTTGTCCAAGTTTGAACTCCCAGATGATGAAATTATCTTTGTTTTGGATGAACTGCGTGACAAGGGAATTGAACTACCACATGATGTGGAAGAGCACATCCATGAGTTCCGTGCTAACCAAGACTTGTCGATCATTGACGAGGACATTGAAGAATTAACCACCAAAAACATCTCTAATCGTGATAAGGTTGATGACAACGTACGTTTCTTTCTAGGTTCGCTCGATTCTTCCAAGATGTTGGACTTTGAGTCTGAGCAGCGAATTGCCAAAGTTTTAAACAGTACTGATGAAGAATCACGCAAGTATGCCATTAATCAACTGGTAACCTCTAACCTTAGGTTAGTGGTCTCCATTGCTAAAAAACACTTGGAAAGAGGATTGGATTTTAATGACCTTATCCAGGAAGGTAACCTAGGACTTTTAAAGGCCATTTCCAAGTTTAACTGGTCATTGGGTAATAAGTTTTCCACTTATGCTACTTGGTGGATTAAACAAGCGATTACTCGTGCTATTGCTGATCAAGCACGTACGGTTCGGATTCCCGTACACATGGTGGAAACTATTAACCGTTTAGCTAAAGCTGAACGTGCCTTAAACCAAGAATTAGGACGCGAACCCACCGCGGAAGAGTTAGCGGAAAAAATGGGTGGTCAAGCTGAAGGGTTTACAGTTAAAAAGATTGCTGAAATTAAACGTTTAAGCTTAGATCCTGTTTCTTTAGATAAAACAGTGGGCCACGATGAAGAATCGCAGTTCGGTGACTTTGTGCGTGATACTGATGCCCAAATGCCCGATGAGTTTACCGAAAGCCGTTCTAATTACGAAAAGATTGATGAGTTGTTAAACAACTGTTTGTCCGAACAGGAAGAATTAATTGTGCGGATGCGCATTGGTATGCCACCTTACAACGAAACCAAAACACTCGATGAGGTAAGTCAAAAGATTAAGATTCCCCGCGAAAAGATTCGGCAAATAGAAACCAAAGCCATTCGCAAACTGCGTCAAGCAGTGCGTAATAACCACATGAGCCTTTCCTTCATGAGGGGAAATGAAAAGAAGGATTAG", "aa": "MSSPKKNFKKPQPKTENQKQALNEERIAELKKSRILGKNRPFKKMIYVDTKAQRKQKHENVAFLKTLQENKESDVPKKRRGRKPKHAPLKEKNNLKLFDILEGSLKSHTENDDTNKVISLLVEVWEKKNKKKDNNSLSNKDIVNVLSKFELPDDEIIFVLDELRDKGIELPHDVEEHIHEFRANQDLSIIDEDIEELTTKNISNRDKVDDNVRFFLGSLDSSKMLDFESEQRIAKVLNSTDEESRKYAINQLVTSNLRLVVSIAKKHLERGLDFNDLIQEGNLGLLKAISKFNWSLGNKFSTYATWWIKQAITRAIADQARTVRIPVHMVETINRLAKAERALNQELGREPTAEELAEKMGGQAEGFTVKKIAEIKRLSLDPVSLDKTVGHDEESQFGDFVRDTDAQMPDEFTESRSNYEKIDELLNNCLSEQEELIVRMRIGMPPYNETKTLDEVSQKIKIPREKIRQIETKAIRKLRQAVRNNHMSLSFMRGNEKKD", "aa_hexdigest": "8bec5144e8d4fcd777528620d647dbbf", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000134830", "ncbi_nrp_id": "WP_010874708.1", "uniref100_id": "UniRef100_P78022", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000134830", "RefSeq:WP_010874708.1", "UniRef:UniRef100_P78022" ] }, "ips": { "uniref100_id": "UniRef100_P78022", "uniref90_id": "UniRef90_P78022" }, "psc": { "uniref90_id": "UniRef90_P78022", "gene": "sigA", "product": "RNA polymerase sigma factor", "uniref50_id": "UniRef50_P78022", "cog_id": "COG0568", "cog_category": "K", "go_ids": [ "GO:0005737", "GO:0003677", "GO:0016987", "GO:0006352" ] }, "pscc": { "uniref50_id": "UniRef50_P78022", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P78022" ], "product": "RNA polymerase sigma factor SigA" }, "genes": [ "sigA" ], "id": "APKECPOBEN_763", "locus": "GCA002090215_00736" }, { "type": "cds", "sequence": "contig_1", "start": 420340, "stop": 420555, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCAAGAGCCTGATTGGGCTGTGTTTAACAGTATTTTAGGGGAACAACAAGCAATGTTTCCAACAACTTTTTTAGCGCAAATTAAGGAGTTCTTTTTAAACAAATCACTCTCATATGACCCTGAGGATTATGAGGAAGATTTACAGTTTTTTCGGCAACTAATTGTGAAACAAAAAGAGCTTTTAAAATATTTTAAAAGCATGGTTGAGCACTAA", "aa": "MQEPDWAVFNSILGEQQAMFPTTFLAQIKEFFLNKSLSYDPEDYEEDLQFFRQLIVKQKELLKYFKSMVEH", "aa_hexdigest": "86b33f35e0a08ef27e88393a2e9f8caa", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8599.727399999998, "isoelectric_point": 4.637345695495605 }, "id": "APKECPOBEN_764", "locus": "GCA002090215_00737" }, { "type": "cds", "sequence": "contig_1", "start": 420730, "stop": 421131, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTTTAGCTGAAGGTAGTGATGCGGTTAAAGCTAAGACTCACAGGGTTTTAAACCTCGTGGAATACTTAGTGGCTTACTTTAAAACCAAGGGCTTTGATGAGCGGATTACCGTCAATAAAGTTATCGACATTATTGCACAAAACCAGAAAGTAACAGCTGACACTAGCTTTAGCCGCTTTTTGTGTCAGAAATTACAGCAATTGTTGCAATACAGTGATGTGGAAACACTGTTCACACAATTACAGCAGCAAAAGCTCAAAGTAAAAGTTAATAAAACTACAACTTTTACCCAAAGAGCCCCGATTTACGAATCAGTGGTTGGTGTGGTTGATAATAGTTTTAGAAATGAATCGCAGCCGGTTGCCATTACCAAGGAGTTTTTAGTCGAAAACAACTGA", "aa": "MFLAEGSDAVKAKTHRVLNLVEYLVAYFKTKGFDERITVNKVIDIIAQNQKVTADTSFSRFLCQKLQQLLQYSDVETLFTQLQQQKLKVKVNKTTTFTQRAPIYESVVGVVDNSFRNESQPVAITKEFLVENN", "aa_hexdigest": "8447be9a46f79bab4d6608b95f274484", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15239.282399999996, "isoelectric_point": 9.013985633850098 }, "id": "APKECPOBEN_765", "locus": "GCA002090215_00738" }, { "type": "cds", "sequence": "contig_1", "start": 421162, "stop": 421563, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08275.17", "PFAM:PF13662.12" ], "nt": "ATGATTCCGATCCATGACCTTAAGGGTAATCCAGTAGGTTTTTCGGGGCGTGCACTGCAAAAAACGGAAAAGATTAAATACAAAAACAGTGCAGAACACCAGTGGTTTAAGAAGAGTGAATTGTTATTTAACTTCCACCGGATTGATAAAAACACACTAAAGCTGTATTTAGTTGAGGGTTACTTCGATGTCTTTGCCCTTACTAGCGCTGGGATTGGTGATGTAGTGGGCTTAATGGGTTTAGCCTTGAGTGAAAGCCACATTATTGCCTTTCAACAGCAACTAAAAGCGCTAGAAACAGTGGTCTTGGCACTCGATAATGATACAGCGGGTCACGATGCTACTTTTAAGCTATTACAAGAGTTAAACGCACACGGCATTATTGTGGAAGTGGTCGATTGA", "aa": "MIPIHDLKGNPVGFSGRALQKTEKIKYKNSAEHQWFKKSELLFNFHRIDKNTLKLYLVEGYFDVFALTSAGIGDVVGLMGLALSESHIIAFQQQLKALETVVLALDNDTAGHDATFKLLQELNAHGIIVEVVD", "aa_hexdigest": "52237bdf68ef7cdbe2c613521183cc7f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08275.17", "name": "DNAG_N", "length": 47, "aa_cov": 0.3533834586466165, "hmm_cov": 1.0, "evalue": 1.1743935930648997e-17, "score": 59.711673736572266, "start": 1, "stop": 47 }, { "id": "PF13662.12", "name": "Toprim_4", "length": 82, "aa_cov": 0.5864661654135338, "hmm_cov": 1.0, "evalue": 4.3458108271784804e-16, "score": 54.42559051513672, "start": 55, "stop": 132 } ], "seq_stats": { "molecular_weight": 14807.884399999984, "isoelectric_point": 6.041268730163575 }, "id": "APKECPOBEN_766", "locus": "GCA002090215_00739" }, { "type": "cds", "sequence": "contig_1", "start": 421963, "stop": 422202, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01807.25" ], "nt": "ATGACCAGTCCCACTAGCCTAGATCAGCTCAAACAGCAAATCAAAATAGCTCCCATAGTGGAGCACTATGCCATTAAGCTAAAAAAGAAGGGGAAGGACTTTGTGGCACTCTGTCCCTTTCATGCTGACCAAAACCCCTCGATGACGGTGTCAGTTGCAAAGAACATCTTTAAGTGTTTCTCGTGTCAGGTGGGCGGTGATGGGATTGCTTTTATCCAAAAAATTGATCAGGTAGATTGA", "aa": "MTSPTSLDQLKQQIKIAPIVEHYAIKLKKKGKDFVALCPFHADQNPSMTVSVAKNIFKCFSCQVGGDGIAFIQKIDQVD", "aa_hexdigest": "3cbbbe92ce72ef01f45600ead0d41a16", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01807.25", "name": "Zn_ribbon_DnaG", "length": 76, "aa_cov": 0.9620253164556962, "hmm_cov": 0.9868421052631579, "evalue": 2.342547142597958e-32, "score": 106.20561218261719, "start": 4, "stop": 79 } ], "seq_stats": { "molecular_weight": 8724.136599999998, "isoelectric_point": 8.86396770477295 }, "id": "APKECPOBEN_767", "locus": "GCA002090215_00740" }, { "type": "cds", "sequence": "contig_1", "start": 422393, "stop": 423124, "strand": "-", "frame": 3, "gene": "glyS", "product": "glycine--tRNA ligase", "db_xrefs": [ "COG:COG0423", "COG:J", "EC:6.1.1.14", "GO:0000166", "GO:0004820", "GO:0005524", "GO:0005737", "GO:0006426", "GO:0046983", "KEGG:K01880", "SO:0001217", "UniRef:UniRef50_P75425", "UniRef:UniRef90_P75425" ], "nt": "TTGTTCCAAACTGAAATTGGCGTTGTCAACAGCGAAAAACGCCTGGTTTACCTTCGTCCGGAAACAGCTCAGGGCATCTTCATTAACTTTAAACAGCTGTTACAACTCAAAAAGCGTCCTTTACCGTTTGGCGTAGCCCAGTTTGGTAAGAGCTTTCGCAATGAAGTGACCCCGGGAAACTTTTTGTTTCGCGTTCGTGAATTTGAACAGTTTGAAATGGAGTGGTTCTGCAATCCTCAAGCCAGTTTAAGCGTTTTTGAAAGCCAACAACAAGCAATTGCTCACTTTTTGTTTAAGGTACTGCAATTAAACCCAGCATTGGTTAAACAATACGAGTATGACAAGAATGAATTAGCACACTATGCCAATAAAACAGTGGATTTTTTATTCCAGTTTCCCCATGGCTTGAGGGAATTGTGGGGTTTAGCTGATCGTGGTACCTTTGATTTAGAACAACACCAAAAGTACGCCAAAAAGCCCTTAGACTTCTTTGATGGAGAGAATAACGAACACTTTATTCCAGCTGTAGTGGAGCCTTCGGTGGGAATTGAGCGCTTGTTTTATGCCTTGATTGTTAGCAGCTATCAACAAGAGCAGTTGGAAGGGGAAATGCGCGAAGTGTTACGTTTACCCTTTCACTTATGTCCAGAACAAATTGTAGTGCTGCCCTTAGTCAATAAACTCAAAGAAACAGCACAAACATTATTTGAAGCGCTGAGTCAAACCCACTGA", "aa": "MFQTEIGVVNSEKRLVYLRPETAQGIFINFKQLLQLKKRPLPFGVAQFGKSFRNEVTPGNFLFRVREFEQFEMEWFCNPQASLSVFESQQQAIAHFLFKVLQLNPALVKQYEYDKNELAHYANKTVDFLFQFPHGLRELWGLADRGTFDLEQHQKYAKKPLDFFDGENNEHFIPAVVEPSVGIERLFYALIVSSYQQEQLEGEMREVLRLPFHLCPEQIVVLPLVNKLKETAQTLFEALSQTH", "aa_hexdigest": "f9147e9b9cf98d319e9b7c8c23760b72", "start_type": "TTG", "rbs_motif": null, "genes": [ "glyS" ], "truncated": "both", "pseudogene": { "start": 422186, "stop": 423424, "observations": { "mutation": [ 422395, 423196, 423310 ], "stop": [ 422395, 423196, 423310 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75425", "query_cov": 1.0, "subject_cov": 0.5412026726057907, "identity": 0.996, "score": 493.0, "evalue": 1.95e-173, "gene_start": 1, "gene_end": 243, "reference_start": 138, "reference_end": 380, "reference_sequence": "MAQVYSQEVYVQYLKRYGFVFQSSELYNGLANSWDFGPLGAVLKQQIKTALYNFFIKNKRDVLLIDTPIILNEQIWKASGHLANFTDALVDCKSCKLRFRVDHLDEQIKSATQWNPKQVNCPNCKANNWSEVRDFNLLFQTEIGVVNSEKRLVYLRPETAQGIFINFKQLLQLKKRPLPFGVAQFGKSFRNEVTPGNFLFRVREFEQFEMEWFCNPQASLSVFESQQQAIAHFLFKVLQLNPALVKQYEYDKNELAHYANKTVDFLFQFPHGLRELWGLADRGTFDLEQHQKYAKKPLDFFDGENNEHFIPAVVEPSVGIERLFYALIVSSYQQEQLEGEMREVLRLPFHLCPEQIVVLPLVNKLKETAQTLFEALSQTHWRIGFESAGSIGKRYRKADAIGTKFAITFDFESLEDQAVTIRERDSLKQVRVPIKELKAWFAQHDDQSH" }, "paralog": false, "identity": 0.9927184466019418, "score": 821.0, "evalue": 6.07e-305, "description": "Internal stop codon at 422395, 423196, 423310. Nonsense mutation around 422395, 423196, 423310.", "psc": { "uniref90_id": "UniRef90_P75425", "gene": "glyS", "product": "glycine--tRNA ligase", "ec_ids": [ "6.1.1.14" ], "uniref50_id": "UniRef50_P75425", "cog_id": "COG0423", "cog_category": "J", "kegg_orthology_id": "K01880", "go_ids": [ "GO:0000166", "GO:0004820", "GO:0005524", "GO:0005737", "GO:0006426", "GO:0046983" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75425", "EC:6.1.1.14", "UniRef:UniRef50_P75425", "COG:COG0423", "COG:J", "KEGG:K01880", "GO:0000166", "GO:0004820", "GO:0005524", "GO:0005737", "GO:0006426", "GO:0046983" ] }, "pscc": { "uniref50_id": "UniRef50_P75425", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75425" ], "product": "Glycine--tRNA ligase" } }, "id": "APKECPOBEN_768", "locus": "GCA002090215_00741" }, { "type": "cds", "sequence": "contig_1", "start": 423308, "stop": 423535, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTCAAGTATATTCCCAAGAAGTGTATGTGCAATACCTCAAGCGCTATGGCTTTGTCTTTCAAAGTAGTGAGCTTTACAATGGACTTGCCAACAGCTGGGACTTCGGTCCGTTGGGTGCCGTTTTAAAGCAACAAATTAAAACGGCACTGTACAACTTCTTCATTAAAAATAAGCGGGATGTATTGTTGATTGACACGCCAATTATTCTCAACGAACAGATCTGA", "aa": "MAQVYSQEVYVQYLKRYGFVFQSSELYNGLANSWDFGPLGAVLKQQIKTALYNFFIKNKRDVLLIDTPIILNEQI", "aa_hexdigest": "8a04040f23482aca9578b1a785557d31", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8752.998099999997, "isoelectric_point": 7.961281394958497 }, "id": "APKECPOBEN_769", "locus": "GCA002090215_00742" }, { "type": "cds", "sequence": "contig_1", "start": 423776, "stop": 424285, "strand": "-", "frame": 3, "gene": "rlmB", "product": "23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB", "db_xrefs": [ "COG:COG0566", "COG:J", "EC:2.1.1.-", "GO:0003723", "GO:0005829", "GO:0006396", "GO:0008173", "GO:0032259", "SO:0001217", "UniRef:UniRef50_P47494", "UniRef:UniRef90_P75424" ], "nt": "ATGAAGCGGATGCAAGAAACCAGTTTTTTATTTGGTAGTAAGGCCTTTTTGGAAGCGTTAGATAACCAACTGCAAATTAAAAAGGTCAATTTATCTGATAAGCACCAAAAGCTGTTGGGCTTAATTAAGAAACGGGGATTGCGGTATGAAATGCACTCCAGCCAGTGGTTTCACCAGCAGTTTCGCCACATTAACCACCAGGAATTTGTTTGTGTAATCAACCCTAACCAAATGCTCAAAACAATTGAGCAGTTAATCCAAATTACAGACAGCAAGTCCACCAGTACCCTGGTAATGCTCCATGAGATCCAAGATCCGCATAACTTTGGCGCCATCTTGCGCACCTGTATGGCAGCTGAAGTGGATGGGATTATCTTTAAAAAGCATAACCAGGCGCCCATTAACAGTACCGTTATTAGAACCAGTATGGGAACTGTCTTTTACCAGAACTTAGTGCAGGTTACTAACCTCAGTTATGCCATTACTACCCTGCAAAAACACGGCTTTTGA", "aa": "MKRMQETSFLFGSKAFLEALDNQLQIKKVNLSDKHQKLLGLIKKRGLRYEMHSSQWFHQQFRHINHQEFVCVINPNQMLKTIEQLIQITDSKSTSTLVMLHEIQDPHNFGAILRTCMAAEVDGIIFKKHNQAPINSTVIRTSMGTVFYQNLVQVTNLSYAITTLQKHGF", "aa_hexdigest": "3d5bf204fed9787cb52d1b8955b69014", "start_type": "ATG", "rbs_motif": "AAAA", "genes": [ "rlmB" ], "truncated": "5-prime", "pseudogene": { "start": 423557, "stop": 424285, "observations": { "mutation": [ 423568, 423778 ], "stop": [ 423568, 423778 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75424", "query_cov": 1.0, "subject_cov": 0.6983471074380165, "identity": 1.0, "score": 335.0, "evalue": 2.52e-115, "gene_start": 1, "gene_end": 169, "reference_start": 1, "reference_end": 169, "reference_sequence": "MKRMQETSFLFGSKAFLEALDNQLQIKKVNLSDKHQKLLGLIKKRGLRYEMHSSQWFHQQFRHINHQEFVCVINPNQMLKTIEQLIQITDSKSTSTLVMLHEIQDPHNFGAILRTCMAAEVDGIIFKKHNQAPINSTVIRTSMGTVFYQNLVQVTNLSYAITTLQKHGFWTVATTLDERLKPKDYRQVDFDKRILLVGNEDKGLNALLLKNADLKVKIPMNPKLNSLNVSVAVGIILFGWKS" }, "paralog": false, "identity": 0.9917355371900827, "score": 472.0, "evalue": 1.04e-171, "description": "Internal stop codon at 423568, 423778. Nonsense mutation around 423568, 423778.", "psc": { "uniref90_id": "UniRef90_P75424", "gene": "rlmB", "product": "23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB", "ec_ids": [ "2.1.1.-" ], "uniref50_id": "UniRef50_P47494", "cog_id": "COG0566", "cog_category": "J", "go_ids": [ "GO:0003723", "GO:0005829", "GO:0006396", "GO:0008173", "GO:0032259" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75424", "EC:2.1.1.-", "UniRef:UniRef50_P47494", "COG:COG0566", "COG:J", "GO:0003723", "GO:0005829", "GO:0006396", "GO:0008173", "GO:0032259" ] }, "pscc": { "uniref50_id": "UniRef50_P47494", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47494" ], "product": "Uncharacterized tRNA/rRNA methyltransferase MG252" } }, "id": "APKECPOBEN_770", "locus": "GCA002090215_00743" }, { "type": "cds", "sequence": "contig_1", "start": 424438, "stop": 424710, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF09190.17" ], "nt": "TTGGATTTAAGCCAATCTTTAATAGAGCAAGCTTGCAGTGATATTAAAAGGATCCAAAAGGCAGTCAATGTGTGCCGCACTTGGTTTGTGTATTCTGAACAATCCGCCATTCCCGCGCCCAAACAGTTTGAACCGGTGTTTAAGGCACTTCTCAATAACCTCAACTTTGCTAATGCCATTACCCACATTTGGAAGTTAGTCAAACAAATTAACCATGATGTAAGCAAGCAAAACTTGAGCGGTTTAAAGGAACATTTAAGCCATTTGGAGTGA", "aa": "MDLSQSLIEQACSDIKRIQKAVNVCRTWFVYSEQSAIPAPKQFEPVFKALLNNLNFANAITHIWKLVKQINHDVSKQNLSGLKEHLSHLE", "aa_hexdigest": "3eff6f093267ab8f71ec489b00920ee5", "start_type": "TTG", "rbs_motif": "AAAA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF09190.17", "name": "DALR_2", "length": 45, "aa_cov": 0.4444444444444444, "hmm_cov": 1.0, "evalue": 4.066373932040501e-06, "score": 22.796756744384766, "start": 48, "stop": 87 } ], "seq_stats": { "molecular_weight": 10315.780699999996, "isoelectric_point": 8.664438056945801 }, "id": "APKECPOBEN_771", "locus": "GCA002090215_00744" }, { "type": "cds", "sequence": "contig_1", "start": 424741, "stop": 424995, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01406.26" ], "nt": "GTGGAGTGTGCCTTTTTAATTGATTACAGCTTTAAAGACCAGTTGACAATTCATGGTGGTGGGGTGGATTTGAAGTTTCCCCATCATGAAAACGAAAACGCAATGCATATGGCACTGTATGACAAACCATTAACGCAGCATTGGATGCACATTGGCCACTTAATGTTTGAAAACCAAAAGATGTCCAAGTCACTGCAAAACTTTCTTTTAGCAGTTGACTTTTTGACAATCCATGACTTTCGCATTTTGCGCTGA", "aa": "MECAFLIDYSFKDQLTIHGGGVDLKFPHHENENAMHMALYDKPLTQHWMHIGHLMFENQKMSKSLQNFLLAVDFLTIHDFRILR", "aa_hexdigest": "16460efba93f13186417598b445de777", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01406.26", "name": "tRNA-synt_1e", "length": 84, "aa_cov": 1.0, "hmm_cov": 1.0, "evalue": 1.2933307999844681e-36, "score": 121.94621276855469, "start": 1, "stop": 84 } ], "seq_stats": { "molecular_weight": 9957.436399999991, "isoelectric_point": 6.196439170837402 }, "id": "APKECPOBEN_772", "locus": "GCA002090215_00745" }, { "type": "cds", "sequence": "contig_1", "start": 425059, "stop": 425601, "strand": "-", "frame": 1, "gene": "cysS", "product": "cysteine--tRNA ligase", "db_xrefs": [ "COG:COG0215", "COG:J", "EC:6.1.1.16", "GO:0004817", "GO:0005524", "GO:0005737", "GO:0005829", "GO:0006423", "GO:0008270", "KEGG:K01883", "SO:0001217", "UniRef:UniRef50_P47495", "UniRef:UniRef90_P75423" ], "nt": "ATGAACCAATTTGAACCTAAATTTACCCTCATTGACACGGTGAGCAATCAGAGCGTGGTATTAGAACAAAAGCAAATTAACATTTATCTGTGTGGACCGACTGTTTACAATGATTTGCACCTAGGCAATACCCGTCCCTTAATTGTGTTTGATGTGCTACAAAGGGTGTTGCAAGCAGCGCAGTACAAGGTGCAGTTTGTCCAAAACATCACTGATATTGACGACAAGATTATCAAGATCGCGCAGCAACAGGAAATTAGTGAAGCGCAGTTGTGCAAACAGCAAATCACCGCTTACAAAAGCTTGTTGAAAAAGCTCAACATTTTGCCGATTAAACACCTCCAAGTCACTGATAAAATTGACAAAATGCCCGGTTACATTGCCCGGTTAGTCAAAAAGGGCTTTGCTTACGTTTCGCCTTTGGGCAACACGTACTTTAGTGTAAGCCAATTACCCCAGTATGGTATTTTAGCCAACCGTGTTGTGGAAACAATTGAGGATGAAGCTACTGACAAACGCAATAAACTGGACTTTGTTTTGTGA", "aa": "MNQFEPKFTLIDTVSNQSVVLEQKQINIYLCGPTVYNDLHLGNTRPLIVFDVLQRVLQAAQYKVQFVQNITDIDDKIIKIAQQQEISEAQLCKQQITAYKSLLKKLNILPIKHLQVTDKIDKMPGYIARLVKKGFAYVSPLGNTYFSVSQLPQYGILANRVVETIEDEATDKRNKLDFVL", "aa_hexdigest": "b013318ac79bf835387a09b1965d5084", "start_type": "ATG", "rbs_motif": null, "genes": [ "cysS" ], "truncated": "5-prime", "pseudogene": { "start": 424756, "stop": 425601, "observations": { "mutation": [ 425001, 425022, 425034, 425061 ], "stop": [ 425001, 425022, 425034, 425061 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75423", "query_cov": 1.0, "subject_cov": 0.41189931350114417, "identity": 1.0, "score": 350.0, "evalue": 2.22e-118, "gene_start": 1, "gene_end": 180, "reference_start": 1, "reference_end": 180, "reference_sequence": "MNQFEPKFTLIDTVSNQSVVLEQKQINIYLCGPTVYNDLHLGNTRPLIVFDVLQRVLQAAQYKVQFVQNITDIDDKIIKIAQQQEISEAQLCKQQITAYKSLLKKLNILPIKHLQVTDKIDKMPGYIARLVKKGFAYVSPLGNTYFSVSQLPQYGILANRVVETIEDEATDKRNKLDFVLWKQTTAGVKWNSPWGWGRPGWHVECAFLIDYSFKDQLTIHGGGVDLKFPHHENENAMHMALYDKPLTQHWMHIGHLMFENQKMSKSLQNFLLAVDFLTIHDFRILRWLFYQKHYYHPLDLSQSLIEQACSDIKRIQKAVNVCRTWFVYSEQSAIPAPKQFEPVFKALLNNLNFANAITHIWKLVKQINHDVSKQNLSGLKEHLSHLEWALNILGIGFKSIHTKLNVQLIKKWASLRKNGQLDKADEVRQKLIKKGLL" }, "paralog": false, "identity": 0.9857651245551602, "score": 555.0, "evalue": 2.53e-201, "description": "Internal stop codon at 425001, 425022, 425034, 425061. Nonsense mutation around 425001, 425022, 425034, 425061.", "psc": { "uniref90_id": "UniRef90_P75423", "gene": "cysS", "product": "cysteine--tRNA ligase", "ec_ids": [ "6.1.1.16" ], "uniref50_id": "UniRef50_P47495", "cog_id": "COG0215", "cog_category": "J", "kegg_orthology_id": "K01883", "go_ids": [ "GO:0004817", "GO:0005524", "GO:0005737", "GO:0005829", "GO:0006423", "GO:0008270" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75423", "EC:6.1.1.16", "UniRef:UniRef50_P47495", "COG:COG0215", "COG:J", "KEGG:K01883", "GO:0004817", "GO:0005524", "GO:0005737", "GO:0005829", "GO:0006423", "GO:0008270" ] }, "pscc": { "uniref50_id": "UniRef50_P47495", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47495" ], "product": "Cysteine--tRNA ligase" } }, "id": "APKECPOBEN_773", "locus": "GCA002090215_00746" }, { "type": "cds", "sequence": "contig_1", "start": 425588, "stop": 426835, "strand": "-", "frame": 3, "gene": "ligA", "product": "NAD-dependent DNA ligase LigA", "db_xrefs": [ "COG:COG0272", "COG:L", "EC:6.5.1.2", "GO:0003677", "GO:0003911", "GO:0005829", "GO:0006260", "GO:0006281", "GO:0006288", "GO:0006310", "GO:0046872", "SO:0001217", "UniRef:UniRef50_P47496", "UniRef:UniRef90_P78021" ], "nt": "GTGTCCAAAGCGGTGGCTAGCTTTGATCGACCCGAAGCATTAATTGAATACCTCGAGCAGTTTGACCAAACACGTGATCAGTTGAACTTCCAAGTCGATGGCCTAGTGGTTAAACTAGATAACTTTCACTTTTACGACCAGTTAGGGTTTACTAGTAAGTTTCCCCGTTGGGCGTTGGCGTACAAGTTTAAACCGAAGTTTGTCCAAACCAAACTGCGTGGTGTCTTGGTAACAGTGGGACGCACTGGCAAGATTAACTATACCGCGCAGTTAGATCCAGTTAACTTAGAGGGGACAGTAGTATCAGCAGCCACACTGCATAACTTTGACTACATTAAGGAGAAGGACATTCGTTTAAACGATACAGTGATAATTTACAAAGCGGGGGAAATTATCCCTAAGGTATTGGAGGTGTGTTTACCGTTAAGAACAGCAGCGAATCAACCGATTCCCGAACAAACCCACTGTCCGGCATGTCAAGCCCCGTTAGTGCAGTATAAGGATGAGGTAGATCAGTACTGTACCAATGAACGTTGTGACCAGCGTAACCTTGAAGCGATTAATTACTTTGTGTCCAAAACGGCTATGGACATCCAGGGGCTAAGTATCCAGACCATTAGTAAGCTGTATGAAAATAACTTAGTGCGCAGCGTCGTTGATTTATACCACTTAAAGGAGCACAAAGCCGCGGTGTTAGCCTTGGAACTTAAAATTGGTGAAGTGCTGTTCAATAAACTAATCAGCAGCATCGAACAGTCCAAAACCAAGGGGATGGCACGCTTGTTAACTGGGCTTGGCATTAAACATGTGGGGCAAGTGTTAGCCAAAAGCTTAACGAAGCACTTTGAAAACATCGATGCCTTACAATCCGCCAGTATGGAAACACTGTTAGAACTCCCTGATGTGGGACCGACTGTGGCAGAATCGTTGTTTAACTGGTTTCACAATGACAATAACTTACAGCTGTTAGCTGCACTTAAAGCAGTTGGAGTGCAAATGGATGCTTTAAAGAGCAACACTAACTTTGACACCGCGAGCATTTACTTTCAAAAGAGTTTTGTTATTACTGGCAGCTTTCCAATTAGTCGCGATACCATTAAAAACTTGCTCGTCAATAAGTATGATTGTCGTTTTACAAATAACGTCACTAGTAAAGTTGATTTTGTGTTAGCTGGCATTAAGGCTACACCTCGTAAACTTGAACAAGCTAAAGCCCTCAATATTCCCATTATTAATGAACCAATTTGA", "aa": "MSKAVASFDRPEALIEYLEQFDQTRDQLNFQVDGLVVKLDNFHFYDQLGFTSKFPRWALAYKFKPKFVQTKLRGVLVTVGRTGKINYTAQLDPVNLEGTVVSAATLHNFDYIKEKDIRLNDTVIIYKAGEIIPKVLEVCLPLRTAANQPIPEQTHCPACQAPLVQYKDEVDQYCTNERCDQRNLEAINYFVSKTAMDIQGLSIQTISKLYENNLVRSVVDLYHLKEHKAAVLALELKIGEVLFNKLISSIEQSKTKGMARLLTGLGIKHVGQVLAKSLTKHFENIDALQSASMETLLELPDVGPTVAESLFNWFHNDNNLQLLAALKAVGVQMDALKSNTNFDTASIYFQKSFVITGSFPISRDTIKNLLVNKYDCRFTNNVTSKVDFVLAGIKATPRKLEQAKALNIPIINEPI", "aa_hexdigest": "80954d33270fa84725ec1594dcd2fe02", "start_type": "GTG", "rbs_motif": null, "genes": [ "ligA" ], "truncated": "both", "pseudogene": { "start": 425582, "stop": 427135, "observations": { "mutation": [ 425590, 426847 ], "stop": [ 425590, 426847 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P78021", "query_cov": 1.0, "subject_cov": 0.6306990881458967, "identity": 0.998, "score": 706.0, "evalue": 5.07e-251, "gene_start": 1, "gene_end": 415, "reference_start": 242, "reference_end": 656, "reference_sequence": "MAKVAQIRAIVEQLKRYDYHYYVLDDPLVSDFEYDQLYKQLQALEQAHPELIQPDSPTQRVGGIVVEAFNKFPHQNPMLSLDNAFDTATIASFIANINNLTGVQNRFVVEPKIDGVSISLTYENGLLTRALTRGDGLVGEDVLSNVKTIKSIPLTIPFHKQVEIRGEIFVDKKTFAQINENLAKPFANARNLAAGTLRNLNSEVSAKRKLKAFFYFVPNALTLGCRSQSAVLAQLKQWNFPVSKAVASFDRPEALIEYLEQFDQTRDQLNFQVDGLVVKLDNFHFYDQLGFTSKFPRWALAYKFKPKFVQTKLRGVLVTVGRTGKINYTAQLDPVNLEGTVVSAATLHNFDYIKEKDIRLNDTVIIYKAGEIIPKVLEVCLPLRTAANQPIPEQTHCPACQAPLVQYKDEVDQYCTNERCDQRNLEAINYFVSKTAMDIQGLSIQTISKLYENNLVRSVVDLYHLKEHKAAVLALELKIGEVLFNKLISSIEQSKTKGMARLLTGLGIKHVGQVLAKSLTKHFENIDALQSASMETLLELPDVGPTVAESLFNWFHNDNNLQLLAALKAVGVQMDALKSNTNFDTASIYFQKSFVITGSFPISRDTIKNLLVNKYDCRFTNNVTSKVDFVLAGIKATPRKLEQAKALNIPIINEPIWT" }, "paralog": false, "identity": 0.9961315280464217, "score": 900.0, "evalue": 0.0, "description": "Internal stop codon at 425590, 426847. Nonsense mutation around 425590, 426847.", "psc": { "uniref90_id": "UniRef90_P78021", "gene": "ligA", "product": "NAD-dependent DNA ligase LigA", "ec_ids": [ "6.5.1.2" ], "uniref50_id": "UniRef50_P47496", "cog_id": "COG0272", "cog_category": "L", "go_ids": [ "GO:0003677", "GO:0003911", "GO:0005829", "GO:0006260", "GO:0006281", "GO:0006288", "GO:0006310", "GO:0046872" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P78021", "EC:6.5.1.2", "UniRef:UniRef50_P47496", "COG:COG0272", "COG:L", "GO:0003677", "GO:0003911", "GO:0005829", "GO:0006260", "GO:0006281", "GO:0006288", "GO:0006310", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_P47496", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47496" ], "product": "DNA ligase" } }, "id": "APKECPOBEN_774", "locus": "GCA002090215_00747" }, { "type": "cds", "sequence": "contig_1", "start": 426845, "stop": 427558, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01653.23", "PFAM:PF22745.1" ], "nt": "ATGGCAAAGGTCGCACAAATTCGAGCAATAGTTGAACAGTTAAAACGCTATGATTACCATTACTATGTTTTGGACGATCCGCTGGTCAGTGACTTTGAATACGACCAGTTATACAAACAGCTGCAAGCGTTGGAACAAGCCCATCCTGAGTTAATCCAACCCGATTCACCAACCCAACGTGTTGGGGGAATTGTAGTGGAAGCATTTAATAAGTTTCCTCACCAAAATCCAATGCTTTCCCTAGACAATGCCTTCGACACAGCAACAATTGCTAGTTTTATTGCCAACATTAACAACTTAACTGGAGTGCAAAACCGCTTTGTGGTGGAACCCAAAATTGATGGTGTAAGCATCTCTTTAACTTACGAAAACGGATTGTTAACTCGGGCTTTAACCCGCGGTGATGGACTTGTGGGTGAGGATGTTTTGAGTAATGTTAAAACGATTAAGAGCATTCCCCTAACGATTCCCTTTCACAAACAGGTGGAAATCCGCGGTGAGATCTTTGTCGATAAAAAAACATTTGCGCAAATTAACGAGAATTTAGCAAAACCGTTTGCCAATGCCCGCAATTTAGCAGCAGGTACACTACGGAACCTCAACAGTGAGGTGAGTGCCAAGCGCAAACTTAAGGCCTTCTTTTACTTTGTTCCCAACGCCTTAACTTTGGGTTGTAGGAGTCAAAGTGCCGTGTTAGCACAGTTAAAGCAGTGA", "aa": "MAKVAQIRAIVEQLKRYDYHYYVLDDPLVSDFEYDQLYKQLQALEQAHPELIQPDSPTQRVGGIVVEAFNKFPHQNPMLSLDNAFDTATIASFIANINNLTGVQNRFVVEPKIDGVSISLTYENGLLTRALTRGDGLVGEDVLSNVKTIKSIPLTIPFHKQVEIRGEIFVDKKTFAQINENLAKPFANARNLAAGTLRNLNSEVSAKRKLKAFFYFVPNALTLGCRSQSAVLAQLKQ", "aa_hexdigest": "fa3048915e09a50043b520f9e07b07fe", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01653.23", "name": "DNA_ligase_aden", "length": 181, "aa_cov": 0.7172995780590717, "hmm_cov": 1.0, "evalue": 4.521423824992924e-68, "score": 224.92138671875, "start": 68, "stop": 237 }, { "id": "PF22745.1", "name": "Nlig-Ia", "length": 37, "aa_cov": 0.15611814345991562, "hmm_cov": 1.0, "evalue": 1.7607908392703994e-08, "score": 30.469568252563477, "start": 17, "stop": 53 } ], "seq_stats": { "molecular_weight": 26510.046300000016, "isoelectric_point": 8.543688774108887 }, "id": "APKECPOBEN_775", "locus": "GCA002090215_00748" }, { "type": "tRNA", "sequence": "contig_1", "start": 427622, "stop": 427695, "strand": "-", "gene": "trnG", "product": "tRNA-Gly(gcc)", "amino_acid": "Gly", "anti_codon": "gcc", "score": 78.9, "nt": "GCAGATATAGTTCAATGGCAGAACATAACCTTGCCAAGGTTAAGATGCGGGTTCGATTCCCGTTATCTGCTCCA", "db_xrefs": [ "SO:0000260" ], "anti_codon_pos": [ 427661, 427663 ], "id": "APKECPOBEN_33", "locus": "GCA002090215_00749" }, { "type": "cds", "sequence": "contig_1", "start": 427726, "stop": 428016, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000B2F78A2", "UniRef:UniRef90_UPI000B2F78A2" ], "nt": "ATGGAAACGCAAAACCAAATAGAAACGTTGCGCTATATTTTTAATCAGCTCAACAACCAAGACAAACCACAAATAATTTGGTTTTCCGGTGAAGGTGAGGATGAAAAAATTAACTTTTTAATCCGTTTGGACAATTATTTCCAACCTACCTTTGTCCAAGACCTTACTATTAACTTTCTCCCCGCTTTTGTTAAGCGAAACAAGAAAAATCCACCCAATACTTTAGCTAAGGGTAACTTTGTCAACATAGCTAATAAGTTATTGGCAGTGTTAGCGCGTTCCTTGAGCTGA", "aa": "METQNQIETLRYIFNQLNNQDKPQIIWFSGEGEDEKINFLIRLDNYFQPTFVQDLTINFLPAFVKRNKKNPPNTLAKGNFVNIANKLLAVLARSLS", "aa_hexdigest": "5b337a5ef0e9e2ad55eaf229c825958c", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 427726, "stop": 428317, "observations": { "insertion": [ 428293 ], "mutation": [ 428014, 428164 ], "stop": [ 428014, 428164 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000B2F78A2", "query_cov": 1.0, "subject_cov": 0.48484848484848486, "identity": 1.0, "score": 192.0, "evalue": 8.67e-61, "gene_start": 1, "gene_end": 96, "reference_start": 1, "reference_end": 96, "reference_sequence": "METQNQIETLRYIFNQLNNQDKPQIIWFSGEGEDEKINFLIRLDNYFQPTFVQDLTINFLPAFVKRNKKNPPNTLAKGNFVNIANKLLAVLARSLSWKQLNKPQQKWLLWLLVPFLLLRQLWLKKKVSKIFQFVNERGILSFIKEQWPILTTLVTVGTTLGTPIFSITISQQKAILENAGHGAFVFLVIFRCLQLRWV" }, "paralog": false, "identity": 0.9897959183673469, "score": 379.0, "evalue": 1.29e-136, "description": "Internal stop codon at 428014, 428164. Frameshift due to insertion around 428293. Nonsense mutation around 428014, 428164.", "psc": { "uniref90_id": "UniRef90_UPI000B2F78A2", "uniref50_id": "UniRef50_UPI000B2F78A2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000B2F78A2", "UniRef:UniRef50_UPI000B2F78A2" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000B2F78A2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000B2F78A2" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 11172.653899999981, "isoelectric_point": 9.098374748229983 }, "id": "APKECPOBEN_776", "locus": "GCA002090215_00750" }, { "type": "cds", "sequence": "contig_1", "start": 428191, "stop": 428883, "strand": "+", "frame": 1, "gene": null, "product": "putative MG255-like protein", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P75422", "UniRef:UniRef90_P75422" ], "nt": "GTGGGTACTACCTTAGGTACACCCATCTTTTCGATTACCATTTCCCAGCAAAAAGCAATTTTGGAAAACGCTGGTCATGGTGCGTTTGTCTTTTTAGTAATCTTTTCGGTGTTTGCAATTGCGCTGGGTTTAGTCTCATCGTTAATCTTTTTAGTCTCATCGTTATTTTCAATTCGTCAAAAAAAGAGCTTACAACAGCTCCACCAAATTCTCTCTCGGTTAATTAACAAGTACTTCTGTTTTGCTAACAGTGAACAAAATCAAACCGGGCGGTACCAACTGAAAAATACCGGGGTGTGCTTCTTTTATGGCTTTGACTTTGAGGAAAAGGAATACATAACCCAAGCGATGAACCTACTGTTGCTCTTGAAGCAAACGAACTGCTTTGTGTTAGTCGGTTGTAAAGAGAGCAACATGTTGTTAATTAAAAACAAGGTGGAACCCGACATTAACCTTAAACAAAGTAGTCTGTACCTTGATTTAAAGAGTCAAATTAGTCCGTTGGCACAAATTAGTAAGTACAACCTCTTGTTTGAAGAGTTGGCCCTGGATGCTGACATGTTTTACTTAGAGGACTTCTTCGCCTTGTTAAAGACTCCGCGGCAAATTGTCAACTTTTTGTTTCGTATAAAACAAAACTTAAAGGAGTTTCACCAACCCCAAACGTTGTGGTTTGACTATTTGGCCTTGTGA", "aa": "MGTTLGTPIFSITISQQKAILENAGHGAFVFLVIFSVFAIALGLVSSLIFLVSSLFSIRQKKSLQQLHQILSRLINKYFCFANSEQNQTGRYQLKNTGVCFFYGFDFEEKEYITQAMNLLLLLKQTNCFVLVGCKESNMLLIKNKVEPDINLKQSSLYLDLKSQISPLAQISKYNLLFEELALDADMFYLEDFFALLKTPRQIVNFLFRIKQNLKEFHQPQTLWFDYLAL", "aa_hexdigest": "371089ef61de6b602dc82df7445e08e3", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 427891, "stop": 429186, "observations": { "mutation": [ 428014, 428164, 428881 ], "stop": [ 428014, 428164, 428881 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75422", "query_cov": 1.0, "subject_cov": 0.4307116104868914, "identity": 0.996, "score": 437.0, "evalue": 4.19e-150, "gene_start": 1, "gene_end": 230, "reference_start": 156, "reference_end": 385, "reference_sequence": "METQNQIETLRYIFNQLNNQDKPQIIWFSGEGEDEKINFLIRLDNYFQPTFVQDLTINFLPAFVKRNKKNPPNTLAKGNFVNIANKLLAVLARSLSWKQLNKPQQKWLLWLLVPFLLLRQLWLKKKVSKIFQFVNERGILSFIKEQWPILTTLVTVGTTLGTPIFSITISQQKAILENAGHGAFVFLVIFSVFAIALGLVSSLIFLVSSLFSIRQKKSLQQLHQILSRLINKYFCFANSEQNQTGRYQLKNTGVCFFYGFDFEEKEYITQAMNLLLLLKQTNCFVLVGCKESNMLLIKNKVEPDINLKQSSLYLDLKSQISPLAQISKYNLLFEELALDADMFYLEDFFALLKTPRQIVNFLFRIKQNLKEFHQPQTLWFDYLALWALVIATDFEFNNVLWSFNDYLSLTNKQKEDYASVNLTAFFNRSLKNHKDNSLLFKPELFNTHAYIPETYTQVTLENIDSDKRAQLVPLNWFSQQKFSDFIEEKINFWQTQQAENKVFYLTLGERIFFLVLVNKKFKQIKLEAALKYLN" }, "paralog": false, "identity": 0.9930394431554525, "score": 841.0, "evalue": 7.39e-312, "description": "Internal stop codon at 428014, 428164, 428881. Nonsense mutation around 428014, 428164, 428881.", "psc": { "uniref90_id": "UniRef90_P75422", "product": "Uncharacterized protein MG255 homolog", "uniref50_id": "UniRef50_P75422", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75422", "UniRef:UniRef50_P75422", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75422", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75422" ], "product": "Uncharacterized protein MG255 homolog" } }, "id": "APKECPOBEN_777", "locus": "GCA002090215_00751" }, { "type": "cds", "sequence": "contig_1", "start": 428923, "stop": 429204, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTGGTCATTTAACGACTATTTAAGTTTAACGAACAAGCAAAAGGAAGATTATGCATCAGTTAACCTCACCGCCTTTTTTAACCGTAGTTTAAAGAACCATAAGGACAACAGCCTGCTCTTTAAACCAGAATTGTTCAACACTCACGCTTATATTCCTGAAACTTACACTCAAGTTACCTTAGAAAACATTGATTCAGACAAACGTGCGCAACTAGTGCCATTGAACTGGTTTTCACAGCAAAAGTTTAGCGATTTTATAGAAGAGAAAATTAACTTTTGA", "aa": "MWSFNDYLSLTNKQKEDYASVNLTAFFNRSLKNHKDNSLLFKPELFNTHAYIPETYTQVTLENIDSDKRAQLVPLNWFSQQKFSDFIEEKINF", "aa_hexdigest": "d14c0244b69e09d57f342a729dca89b5", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11118.29529999999, "isoelectric_point": 5.575132179260252 }, "id": "APKECPOBEN_778", "locus": "GCA002090215_00752" }, { "type": "cds", "sequence": "contig_1", "start": 429229, "stop": 429330, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTTTACCTCACTTTGGGCGAGCGGATCTTCTTTTTAGTATTAGTTAACAAGAAGTTCAAACAAATTAAACTAGAAGCAGCGTTGAAGTACCTCAACTAA", "aa": "MFYLTLGERIFFLVLVNKKFKQIKLEAALKYLN", "aa_hexdigest": "80607b1e5c74462264a515b250e1e760", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3992.853900000001, "isoelectric_point": 9.873542594909669 }, "id": "APKECPOBEN_779", "locus": "GCA002090215_00753" }, { "type": "tRNA", "sequence": "contig_1", "start": 429331, "stop": 429405, "strand": "+", "gene": "trnR", "product": "tRNA-Arg(cct)", "amino_acid": "Arg", "anti_codon": "cct", "score": 55.1, "nt": "CTTCCCTTGGTGTAATGGATAACACGACTGAGTCCTAATCAGTAAATGGAGGTTCGATTCCTCTAGGGAAGGCCA", "db_xrefs": [ "SO:0001036" ], "anti_codon_pos": [ 429335, 429337 ], "id": "APKECPOBEN_10", "locus": "GCA002090215_00754" }, { "type": "tRNA", "sequence": "contig_1", "start": 429479, "stop": 429552, "strand": "+", "gene": "trnW", "product": "tRNA-Trp(cca)", "amino_acid": "Trp", "anti_codon": "cca", "score": 74.2, "nt": "GGGGGTGTAGTTTAGTGGCAGAACAACAGTCTCCAAAACTGTCTGTGTGGGTTCGATTCCTTCCACCCCCGCCA", "db_xrefs": [ "SO:0000271" ], "anti_codon_pos": [ 429511, 429513 ], "id": "APKECPOBEN_11", "locus": "GCA002090215_00755" }, { "type": "cds", "sequence": "contig_1", "start": 429560, "stop": 429952, "strand": "+", "frame": 2, "gene": null, "product": "putative MG256-like protein", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P47498", "UniRef:UniRef90_P75421" ], "nt": "ATGAATTACAGTTACAGCTTTAAAGAATACATCGAACGGTTTGCCAAAAAGGTTAATTCGATTGACAGCGAGTACTTCGAATTTTCCTCTTACATAGAGCGCATGCGCACTGTGTTTGGTTTACTGATTGCACTGATATGCTTTAGTAACGTTCTCTGTTTTCTTTTTATTGCAACTTGGTTTAGCACAAAGGGATTTGGTCAACACTACCGGGCGTTAATATTTACCTTGTTTATTCCCTTTGTCACTTCACTTTTAGCCAACATTATCTTCATTAACCTTAATCGCGCTTTTCGCGAATACTTTAAGATCAGCAGTAAAAGCCGTTCTTTTTTGGTAATCTGTGCCTTCTCCTCATTACCGATTGTGAACATCTGGCTAATGTTGTGGTGA", "aa": "MNYSYSFKEYIERFAKKVNSIDSEYFEFSSYIERMRTVFGLLIALICFSNVLCFLFIATWFSTKGFGQHYRALIFTLFIPFVTSLLANIIFINLNRAFREYFKISSKSRSFLVICAFSSLPIVNIWLMLW", "aa_hexdigest": "9ba2b9b74d30dccfdea4641c97b659ae", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 429560, "stop": 430255, "observations": { "mutation": [ 429950 ], "stop": [ 429950 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75421", "query_cov": 1.0, "subject_cov": 0.5038759689922481, "identity": 1.0, "score": 249.0, "evalue": 5.36e-82, "gene_start": 1, "gene_end": 130, "reference_start": 1, "reference_end": 130, "reference_sequence": "MNYSYSFKEYIERFAKKVNSIDSEYFEFSSYIERMRTVFGLLIALICFSNVLCFLFIATWFSTKGFGQHYRALIFTLFIPFVTSLLANIIFINLNRAFREYFKISSKSRSFLVICAFSSLPIVNIWLMLWWVAMIKRFTSNYAFAIFDKYNGLTSGVFIFDFADNVNFEGKLVSFDNTKDTNRDFVHFYSEAKLKRDKITLQTNPIPHERMYVNRMYYQQKLSMGANQNSPSTAFANLKRYVEHKQQKIIKIKQFILT" }, "paralog": false, "identity": 0.9956709956709957, "score": 465.0, "evalue": 3.25e-169, "description": "Internal stop codon at 429950. Nonsense mutation around 429950.", "psc": { "uniref90_id": "UniRef90_P75421", "product": "Uncharacterized protein MG256 homolog", "uniref50_id": "UniRef50_P47498", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75421", "UniRef:UniRef50_P47498", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47498", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47498" ], "product": "Uncharacterized protein MG256" } }, "id": "APKECPOBEN_780", "locus": "GCA002090215_00756" }, { "type": "cds", "sequence": "contig_1", "start": 429959, "stop": 430336, "strand": "+", "frame": 2, "gene": null, "product": "putative MG256-like protein", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P47498", "UniRef:UniRef90_P75421" ], "nt": "ATGATTAAGCGCTTTACTAGTAACTATGCCTTTGCTATCTTTGATAAGTACAACGGTTTGACTAGTGGTGTGTTTATCTTTGACTTTGCTGATAATGTCAATTTTGAGGGTAAACTAGTGAGCTTTGACAATACCAAGGACACCAACCGTGACTTTGTCCACTTTTACAGTGAGGCCAAACTTAAACGCGATAAAATTACGCTACAAACGAATCCAATTCCACACGAAAGGATGTATGTTAACCGCATGTACTATCAGCAAAAGCTCAGTATGGGTGCTAACCAAAACAGCCCTTCAACGGCATTTGCTAACTTAAAGCGGTATGTGGAACATAAACAGCAAAAAATTATCAAGATTAAGCAGTTTATCTTGACCTAA", "aa": "MIKRFTSNYAFAIFDKYNGLTSGVFIFDFADNVNFEGKLVSFDNTKDTNRDFVHFYSEAKLKRDKITLQTNPIPHERMYVNRMYYQQKLSMGANQNSPSTAFANLKRYVEHKQQKIIKIKQFILT", "aa_hexdigest": "96c3cce8e61a487fbe8823ac25ab6f0f", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 429659, "stop": 430336, "observations": { "mutation": [ 429950 ], "stop": [ 429950 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75421", "query_cov": 1.0, "subject_cov": 0.4844961240310077, "identity": 1.0, "score": 247.0, "evalue": 5.05e-81, "gene_start": 1, "gene_end": 125, "reference_start": 134, "reference_end": 258, "reference_sequence": "MNYSYSFKEYIERFAKKVNSIDSEYFEFSSYIERMRTVFGLLIALICFSNVLCFLFIATWFSTKGFGQHYRALIFTLFIPFVTSLLANIIFINLNRAFREYFKISSKSRSFLVICAFSSLPIVNIWLMLWWVAMIKRFTSNYAFAIFDKYNGLTSGVFIFDFADNVNFEGKLVSFDNTKDTNRDFVHFYSEAKLKRDKITLQTNPIPHERMYVNRMYYQQKLSMGANQNSPSTAFANLKRYVEHKQQKIIKIKQFILT" }, "paralog": false, "identity": 0.9955555555555555, "score": 450.0, "evalue": 1.63e-163, "description": "Internal stop codon at 429950. Nonsense mutation around 429950.", "psc": { "uniref90_id": "UniRef90_P75421", "product": "Uncharacterized protein MG256 homolog", "uniref50_id": "UniRef50_P47498", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75421", "UniRef:UniRef50_P47498", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47498", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47498" ], "product": "Uncharacterized protein MG256" } }, "id": "APKECPOBEN_781", "locus": "GCA002090215_00757" }, { "type": "cds", "sequence": "contig_1", "start": 430381, "stop": 430674, "strand": "+", "frame": 1, "gene": "rpmE", "product": "50S ribosomal protein L31", "db_xrefs": [ "COG:COG0254", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904", "RefSeq:WP_010874716.1", "SO:0001217", "UniParc:UPI0000133F18", "UniRef:UniRef100_P78020", "UniRef:UniRef50_P47499", "UniRef:UniRef90_P78020" ], "nt": "ATGAAAAAAGATTTTCATTTTCCAAGCCAATCCGTTAGCTTCAAGTGTGCTTCTTGCAGCAATAGTTTCACCATTGAATCAACCCTAAAACAAAAAGAAATTACGATTGATATTTGTGGTAAATGTCACCCGTTTTACATTGGTGAATTAACCAAACAAACAGTCCACGGTCGTGCGGAAAAGCTTTCTGGAAAGTTCAATGCTGGCAAAGCCTTTTTAGAGAATAAAACTCCTAAAAAAGCGAAAGGCAAAACAGAAGAATATACCAAACACCGTTCTTTAAACGAACTGTAG", "aa": "MKKDFHFPSQSVSFKCASCSNSFTIESTLKQKEITIDICGKCHPFYIGELTKQTVHGRAEKLSGKFNAGKAFLENKTPKKAKGKTEEYTKHRSLNEL", "aa_hexdigest": "3c44c7a45b1bd5b7f6d5e80e4c2c5463", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000133F18", "ncbi_nrp_id": "WP_010874716.1", "uniref100_id": "UniRef100_P78020", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000133F18", "RefSeq:WP_010874716.1", "UniRef:UniRef100_P78020" ] }, "ips": { "uniref100_id": "UniRef100_P78020", "uniref90_id": "UniRef90_P78020" }, "psc": { "uniref90_id": "UniRef90_P78020", "gene": "rpmE", "product": "50S ribosomal protein L31", "uniref50_id": "UniRef50_P47499", "cog_id": "COG0254", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_P47499", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47499" ], "product": "Large ribosomal subunit protein bL31" }, "genes": [ "rpmE" ], "id": "APKECPOBEN_782", "locus": "GCA002090215_00758" }, { "type": "cds", "sequence": "contig_1", "start": 430675, "stop": 431115, "strand": "+", "frame": 1, "gene": "prfA", "product": "peptide chain release factor 1", "db_xrefs": [ "COG:COG0216", "COG:J", "GO:0003747", "GO:0005737", "GO:0006415", "GO:0016149", "KEGG:K02835", "SO:0001217", "UniRef:UniRef50_P47500", "UniRef:UniRef90_P75420" ], "nt": "GTGGAGTTTGACAAGCAGTTCTTTTCTAGTGTTGAAAAGATTGTTGAACTTGCTGAACAACTCGAAAAGGACTTAAATAAACCCGATTTGACCTTTGAACAGATCAAAGCGATCAACAAGGAACTGAAACATAAACAACCCTTAGTTGTTAAATTCAAAGAATTTAAACGGCTCATAGATCAAGCTTTGGAAGCGGAGGCCATTTTGGAAAACAACGAGCTCAAGGAGCTACATGATGAGGCCAAAAAAGAGCTGGAACGGGTGCGCAGTGTTGTCCCTGAATACGAAGAAGCGTTAAAACTGTTGTTATTACCAATTGATGAGAATAACCAGAAAAACGTGATTGTAGAGCTTCGTCCCGCTGCTGGTGGGGATGAATCGTGCATCTTTTTAGCTGATTTATTCAATATGTACCGCAACTTTTGTACTAACAAGGGTTGA", "aa": "MEFDKQFFSSVEKIVELAEQLEKDLNKPDLTFEQIKAINKELKHKQPLVVKFKEFKRLIDQALEAEAILENNELKELHDEAKKELERVRSVVPEYEEALKLLLLPIDENNQKNVIVELRPAAGGDESCIFLADLFNMYRNFCTNKG", "aa_hexdigest": "c0c64d44ddf7ae8abd90fcd93b1ef3eb", "start_type": "GTG", "rbs_motif": null, "genes": [ "prfA" ], "truncated": "3-prime", "pseudogene": { "start": 430675, "stop": 431418, "observations": { "mutation": [ 431113 ], "stop": [ 431113 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75420", "query_cov": 1.0, "subject_cov": 0.40668523676880225, "identity": 0.993, "score": 272.0, "evalue": 2.81e-89, "gene_start": 1, "gene_end": 146, "reference_start": 1, "reference_end": 146, "reference_sequence": "MEFDKQFFSSVEKIVELAEQLEKDLNKPDLTFEQIKAINKELKHKQPLVVKFKEFKRLIDQALEAEAILENNELKELHDEAKKELERVRSVVPEYEEALKLLLLPIDENNQKNVIVELRPAAGGDESCIFLADLFNMYRNFCSNKGWKLQINEMIPSSVGLNFVSFEVNGVDVFAKLKFESGVHRVQRVPATESKGRVHTSTVTVAVLPQLEAVEVHINPADLRVDTYRASGAGGQHVNRTESAVRITHLPTGIVVSCQEGKSQFTNRDTAMKMLRAKLWEKAQNEQLSTQAGLRKSQVGSGDRAEKIRTYNYPQNRVTDHRIKLTVNKLNTIILGDLDEIIEALQADEKKQQLENFFS" }, "paralog": false, "identity": 0.9878542510121457, "score": 475.0, "evalue": 1.78e-171, "description": "Internal stop codon at 431113. Nonsense mutation around 431113.", "psc": { "uniref90_id": "UniRef90_P75420", "gene": "prfA", "product": "peptide chain release factor 1", "uniref50_id": "UniRef50_P47500", "cog_id": "COG0216", "cog_category": "J", "kegg_orthology_id": "K02835", "go_ids": [ "GO:0003747", "GO:0005737", "GO:0006415", "GO:0016149" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75420", "UniRef:UniRef50_P47500", "COG:COG0216", "COG:J", "KEGG:K02835", "GO:0003747", "GO:0005737", "GO:0006415", "GO:0016149" ] }, "pscc": { "uniref50_id": "UniRef50_P47500", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47500" ], "product": "Peptide chain release factor 1" } }, "id": "APKECPOBEN_783", "locus": "GCA002090215_00759" }, { "type": "cds", "sequence": "contig_1", "start": 431134, "stop": 431754, "strand": "+", "frame": 1, "gene": null, "product": "Peptide chain release factor 1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1W9TI19" ], "nt": "ATGATCCCATCCAGTGTCGGGTTAAACTTCGTTTCCTTTGAAGTTAATGGTGTCGATGTGTTTGCCAAGCTCAAGTTTGAATCTGGCGTACACCGGGTACAACGCGTACCCGCTACTGAATCCAAGGGGAGAGTACACACTTCAACCGTTACGGTTGCTGTTTTACCACAACTAGAAGCGGTTGAAGTTCATATTAACCCAGCTGATCTTAGAGTAGATACCTACCGTGCTTCGGGTGCTGGGGGTCAACACGTTAACCGGACTGAAAGTGCAGTGCGGATTACCCATTTACCAACTGGCATAGTAGTTTCGTGTCAAGAGGGTAAATCACAATTTACTAACCGTGATACGGCAATGAAGATGCTACGTGCTAAACTGTGGGAAAAAGCGCAGAACGAACAGTTATCAACCCAAGCAGGTTTGCGTAAAAGTCAGGTTGGGTCAGGTGATCGTGCCGAAAAGATCCGCACATATAACTATCCCCAAAACCGGGTCACAGACCACCGCATTAAACTTACCGTCAATAAGCTTAACACGATTATTTTAGGTGACCTTGATGAAATTATTGAAGCGTTGCAGGCAGATGAAAAGAAACAACAACTAGAGAACTTTTTCAGTTAA", "aa": "MIPSSVGLNFVSFEVNGVDVFAKLKFESGVHRVQRVPATESKGRVHTSTVTVAVLPQLEAVEVHINPADLRVDTYRASGAGGQHVNRTESAVRITHLPTGIVVSCQEGKSQFTNRDTAMKMLRAKLWEKAQNEQLSTQAGLRKSQVGSGDRAEKIRTYNYPQNRVTDHRIKLTVNKLNTIILGDLDEIIEALQADEKKQQLENFFS", "aa_hexdigest": "76d49743f1eca11ec3aa76929feb9462", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A1W9TI19", "query_cov": 0.9660194174757282, "subject_cov": 0.8223140495867769, "identity": 0.643, "score": 248.0, "evalue": 1.85e-80, "valid": false, "gene": "prfA", "product": "peptide chain release factor 1", "uniref50_id": "UniRef50_A0A1W9TI19", "kegg_orthology_id": "K02835", "go_ids": [ "GO:0003747", "GO:0006415" ] }, "pscc": { "uniref50_id": "UniRef50_A0A1W9TI19", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1W9TI19" ], "product": "Peptide chain release factor 1" }, "genes": [], "id": "APKECPOBEN_784", "locus": "GCA002090215_00760" }, { "type": "cds", "sequence": "contig_1", "start": 431754, "stop": 431879, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACTTGTATGAACTTTTCTTGAACCAAAAGCTGCTGTATGGCACCGATCCGCACTTTAACGGTGTTTTTCTCACCCTATTAGAAAAGTTTGGTCTGCACTTTAAGGATTTAACAGCACTGTGA", "aa": "MNLYELFLNQKLLYGTDPHFNGVFLTLLEKFGLHFKDLTAL", "aa_hexdigest": "f75fee177eed71695c8a8abee943c0ea", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4815.5853, "isoelectric_point": 5.977779579162598 }, "id": "APKECPOBEN_785", "locus": "GCA002090215_00761" }, { "type": "cds", "sequence": "contig_1", "start": 432366, "stop": 432791, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGCTGATGGTAACGGAATTTTGTTTTACCAAAAGCTGTATAACTTTTTATTGGGTAACCGCCAAGTTAAACAAGTGATACTGGAGTGCTCACCAACCCAGAAAAAAGAGTTTTTAGCCTTATTTAGCATTTTTAAAACTAGTGAAATTTACACAAGCCACAAGCAATTCATCGGTTTGAGTATTGACAACACTAAACTACCAGTCTTAAAAATTGCGCAAACCAAGCAAATTAAAGCACTGCTAGACAAGGGGATGACGGCCATTATCCCCACCGACACGCAAATAGGATTAATGAGTTATTGCCAACAAGATTTAGACCACATTAAACAACGCGATCCCAACAAGCACTACGTCCAGTTTTTAGCACCTAGTCAAATTAACCAGTTACCCAAACAATTACAAAAGCTAGCCAAACTGTTTTGA", "aa": "MADGNGILFYQKLYNFLLGNRQVKQVILECSPTQKKEFLALFSIFKTSEIYTSHKQFIGLSIDNTKLPVLKIAQTKQIKALLDKGMTAIIPTDTQIGLMSYCQQDLDHIKQRDPNKHYVQFLAPSQINQLPKQLQKLAKLF", "aa_hexdigest": "6a44a0a2d0cf2713a58c0cbc4ca36782", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16171.81199999998, "isoelectric_point": 9.543722343444824 }, "id": "APKECPOBEN_786", "locus": "GCA002090215_00762" }, { "type": "cds", "sequence": "contig_1", "start": 432960, "stop": 433115, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCAGCAGAACTGTTTTATTGTGCAAAACAGCTTTAAAAGTAATAACGAACCGTCCTTAATTTACAACTTGGATACGAAGCAAATTGTTCGTGGTAGTTCAACCCAATTACAACGTTTCCAAGCGTTACTAGCAAAACACAAATTAAGACACTAA", "aa": "MQQNCFIVQNSFKSNNEPSLIYNLDTKQIVRGSSTQLQRFQALLAKHKLRH", "aa_hexdigest": "5629f11c7c49c8c20d46cdc6852e5a84", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5974.7895, "isoelectric_point": 10.182410621643069 }, "id": "APKECPOBEN_787", "locus": "GCA002090215_00763" }, { "type": "cds", "sequence": "contig_1", "start": 433247, "stop": 433555, "strand": "+", "frame": 2, "gene": null, "product": "P68 family surface lipoprotein", "db_xrefs": [ "GO:0005886", "GO:0009897", "SO:0001217", "UniParc:UPI000013A87E", "UniRef:UniRef100_P75418", "UniRef:UniRef50_P75280", "UniRef:UniRef90_P75418" ], "nt": "ATGAAATTTAAGTATGGTGCTACCCTTTTTAGTGGCTTTTTAGGGCTTTCAGCGATCCTAGCTGCTTGTGGTGCAAAGGGTAAATTTGACCAAGTTGATGATGGCAAAATTGTGTTAGCCTCTTCATTAACTTCTAAAAATGCCGCTAATGCTCTACAAGCTGTGGTGGAAAAATACAACCAGGTTAAGGGTGGCAATGATTATCCTATTGAAATTACCCAAATTACCGGTGGTTATGACGGTGGACGTGGTAACTTACAAACCAAACTTAGCGTTAAAGACAAAACGACCTTTTACAACCTCATCTAA", "aa": "MKFKYGATLFSGFLGLSAILAACGAKGKFDQVDDGKIVLASSLTSKNAANALQAVVEKYNQVKGGNDYPIEITQITGGYDGGRGNLQTKLSVKDKTTFYNLI", "aa_hexdigest": "014dc7451662ad81bfb87e93f43c0065", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013A87E", "uniref100_id": "UniRef100_P75418", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013A87E", "UniRef:UniRef100_P75418" ] }, "ips": { "uniref100_id": "UniRef100_P75418", "uniref90_id": "UniRef90_P75418" }, "psc": { "uniref90_id": "UniRef90_P75418", "product": "P68 family surface lipoprotein", "uniref50_id": "UniRef50_P75280", "go_ids": [ "GO:0005886", "GO:0009897" ] }, "pscc": { "uniref50_id": "UniRef50_P75280", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75280" ], "product": "Uncharacterized lipoprotein MPN_506" }, "genes": [], "id": "APKECPOBEN_788", "locus": "GCA002090215_00764" }, { "type": "cds", "sequence": "contig_1", "start": 433594, "stop": 433881, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAACTGCCACTAGACAACGTTGATCTCTCCAAGTTATCACCTAACTTTTTGAAGTTCAACGAACGCATTAGCGGTGTAGCCAAAAAGGCCAATTACGCCATTCCCGTATCACTTTCAACTGAAGTTTTAGCTTTAAATGGACCGGTATTACACTATATCTTGAACAGCGCGAAGGGTGGTAATGAAACAGCTGTAACACAAAGAACTGCTAGCACAAACCAACAAAATACACTTCAAAATTCGTTAAAAATTAACGAAAGCGACCCCATAACCAAAGAGCTTTGA", "aa": "MELPLDNVDLSKLSPNFLKFNERISGVAKKANYAIPVSLSTEVLALNGPVLHYILNSAKGGNETAVTQRTASTNQQNTLQNSLKINESDPITKEL", "aa_hexdigest": "484cdd45e9590026773558c21cf07e4c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10338.585999999988, "isoelectric_point": 6.554865837097168 }, "id": "APKECPOBEN_789", "locus": "GCA002090215_00765" }, { "type": "cds", "sequence": "contig_1", "start": 434074, "stop": 435627, "strand": "+", "frame": 1, "gene": null, "product": "P80 family lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001330F80B" ], "nt": "GTGGAGAATGGCCTTAAGGGTTATACCTTTAAAGCTAGTGTCTTTGAAAACTGGCATGAACTGCTCGATTTTTCCGCAAGGATAGCTAGATCATTTACTAATATTCACACCAAAGAAACCAAAAAGGGAACGGACATTCAAGGTGTACTTGGGATTGACAGTACACCTAACGCCTTATTTACTTCAGTATTTGCTGCAGGTGGTGGTAACTATGACAACTTCTTTTATAAGGTGCAAGATGGTCGGGCTGACTTTAGTAACTTTAAAAATAAGGAAACCTCTTACCAAAACTTACAAAAAGTATTTAGCGATTATAAGGAGTTAATTGATAAAAACGGTTTGTTTGTCAATAAGGGTGGCTCTTACTCTTCTAACTTCCAGAAGTTCCACCAATTAGCTTACAGTATTTCTTCCACTTCAGGTTTCTTTTATTCGTTTGCTGGTAACAGTGCTAAACGCTTAAAGTTTGATGACGGTAACTTTATTGAGTTTCCGCGCTTCACTCAACCAGTTAAAGCGCCTAATAAAACAACTCAAAGTGGTGGAGAAAATAACAATAAAAGTGAAAATAATCTTCTTGGAACTTTTGAAATAGAAGACAAAAGCAAAAAGCGCGAAAGCGTTGAATCTAGTGAAAAATCAAGTTCTGGGACTCAATCCAAAAGCAAGAAAGGTAAAAGTATGAAAACCATTTACCTTTATAAAGCACAAATTCCAGAAGGAAAAAACAAAGGTGATAATGCCATCTTAATTACTAACCAAACTGTTATTGATAAACTAGAAAAAGCTGCTAAAGAAGACGGTAAAAGTGGAACTCAAGTTTCAACTATTGCAAGTAAATCAACAACTAAAGAGAACCAAGAAAACAAAATAATTGGTTACACATCGACTGATAACCTTCGTGAAGACAATAAGAACATTTTCGATATCGATGATCTTAACGATGAAAAATATGACCGTAAGATTATTGTTAGTGCTACTGAAGAAGTATTGGAACAATCTAACACTTTACAGAGTGAAGAAGCAGTTGTTCTACCGGCACCTGCTAAATATAAAGCTAGTGATTCCACAAAAATAGCAATCACCCAAGAACCTAACCTAATTGGTATCCATGCTAACGAAAAGGAAAATGAGGAAACACGTAAGTTTGTTAATTGGTTTTTAAACAGTGAGCAAACTTGGGAATCCAAGAAATACAGTAAAAATAAGCAAAACTCCAACGAAAAGAGAACTCCTGCACAATTCTTTGCTGATTCCGCGTCTTACATTCTACCCTTAAAGAAAACCTTCGATAATGCCAACAAAAAAGAAGATCAAAGTAAGGCCAATAACAAGACTAATAGCCAAAGAACTAACACCTATATCGAAAAGGCATTAGAAATTTTTAAAGGAATTAGCCAAAACCAAATAACAAGTTACAGTGATCCAAGCGACTTTAGAAGTGGTCGCTTCCGCGATGGTATTGGTTCTAACTTTAATGCAGTAGTAAATTCCAAAGTGGATTTCAATAAATTTATTAATAACTTTATTGCTAGTCTAGGTTCTGACATCTAA", "aa": "MENGLKGYTFKASVFENWHELLDFSARIARSFTNIHTKETKKGTDIQGVLGIDSTPNALFTSVFAAGGGNYDNFFYKVQDGRADFSNFKNKETSYQNLQKVFSDYKELIDKNGLFVNKGGSYSSNFQKFHQLAYSISSTSGFFYSFAGNSAKRLKFDDGNFIEFPRFTQPVKAPNKTTQSGGENNNKSENNLLGTFEIEDKSKKRESVESSEKSSSGTQSKSKKGKSMKTIYLYKAQIPEGKNKGDNAILITNQTVIDKLEKAAKEDGKSGTQVSTIASKSTTKENQENKIIGYTSTDNLREDNKNIFDIDDLNDEKYDRKIIVSATEEVLEQSNTLQSEEAVVLPAPAKYKASDSTKIAITQEPNLIGIHANEKENEETRKFVNWFLNSEQTWESKKYSKNKQNSNEKRTPAQFFADSASYILPLKKTFDNANKKEDQSKANNKTNSQRTNTYIEKALEIFKGISQNQITSYSDPSDFRSGRFRDGIGSNFNAVVNSKVDFNKFINNFIASLGSDI", "aa_hexdigest": "3098d15a91ac07d37940ff92bc31a417", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001330F80B", "query_cov": 0.8588007736943907, "subject_cov": 0.9466950959488273, "identity": 0.588, "score": 472.0, "evalue": 2.9e-160, "valid": false, "product": "P80 family lipoprotein", "uniref50_id": "UniRef50_UPI001330F80B" }, "pscc": { "uniref50_id": "UniRef50_UPI001330F80B", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001330F80B" ], "product": "P80 family lipoprotein" }, "genes": [], "id": "APKECPOBEN_790", "locus": "GCA002090215_00766" }, { "type": "cds", "sequence": "contig_1", "start": 435665, "stop": 435844, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01420.25" ], "nt": "GTGGAGATTAAAACTTACAAAATCAAAGATATTTGCGAGATTTCTAGGGGCAAAGCAATTACCAAAGAATACATAAGAGCTAACCCTGGTGAATATCCTGTTTATTCTGGTTCAACATTAAATGATGGTGAAATAGGTCGCATAGATGAATGCGAATTTGATGGTGAATATGTAACTTGA", "aa": "MEIKTYKIKDICEISRGKAITKEYIRANPGEYPVYSGSTLNDGEIGRIDECEFDGEYVT", "aa_hexdigest": "6dc7da85cb8446a93bd914f3b9f7c3ac", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01420.25", "name": "Methylase_S", "length": 61, "aa_cov": 0.9661016949152542, "hmm_cov": 0.9836065573770492, "evalue": 2.8286824621084878e-11, "score": 39.35646438598633, "start": 3, "stop": 59 } ], "seq_stats": { "molecular_weight": 6710.4442, "isoelectric_point": 4.644507408142091 }, "id": "APKECPOBEN_791", "locus": "GCA002090215_00767" }, { "type": "cds", "sequence": "contig_1", "start": 435971, "stop": 436384, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01420.25" ], "nt": "ATGGAAGCGCCTAAACATGTTAATAATGCTTGTGTTATTCCTAATCTAACTTTAAAAAAGATGAGAGAAATAGAACTAGATTTTCCAAGCAAGAAAATTCAAGAGAAAATTGCTACTATCTTAGATACTTTTACCGAGCTAAGCGCCGAGCTAAGAGAGCGAAAGAAGCAGTATGCCTTCTATCGCGATTATCTGCTCAACCAAGAAAATATCCGAAAAATTTACGGAGCAAATATACCTTTCGAAACTTTTCAAGTAAAGGATATTTGTGAAATTAGAAGAGGTCGAGCGATCACTAAAGCGTATATTAGGAATAATCCTGGAGAAAATCCAGTTTATTCCGCAGCTACCACAAATGATGGTGAGTTAGGACGTATTAAAGATTGCGATTTTGATGGTGAATACATCACTTGA", "aa": "MEAPKHVNNACVIPNLTLKKMREIELDFPSKKIQEKIATILDTFTELSAELRERKKQYAFYRDYLLNQENIRKIYGANIPFETFQVKDICEIRRGRAITKAYIRNNPGENPVYSAATTNDGELGRIKDCDFDGEYIT", "aa_hexdigest": "76b6e8516d96bbb35b19364825e78b37", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01420.25", "name": "Methylase_S", "length": 62, "aa_cov": 0.4233576642335766, "hmm_cov": 0.9838709677419355, "evalue": 4.33602857444563e-21, "score": 71.28997039794922, "start": 80, "stop": 137 } ], "seq_stats": { "molecular_weight": 15910.972899999979, "isoelectric_point": 7.65106258392334 }, "id": "APKECPOBEN_792", "locus": "GCA002090215_00768" }, { "type": "cds", "sequence": "contig_1", "start": 436571, "stop": 436738, "strand": "+", "frame": 2, "gene": null, "product": "restriction endonuclease subunit S", "db_xrefs": [ "EC:3.1.21.3", "KEGG:K01154", "SO:0001217", "UniRef:UniRef50_UPI00190F3D87", "UniRef:UniRef90_UPI00190F3D87" ], "nt": "ATGGCTGAAATCGAACTCTCTTTTCCACCTCTAGAAATTCAAGAAAAGATTGCTGATATTCTCTTTGCCTTTGAAAAACTTTGCAATGATTTAGTAGAAGGTATTCCTGCTGAAGTCGAAATGCGTAAAAAGCAATTGGATTACTATCAGAACTTCTTATTTAATTGA", "aa": "MAEIELSFPPLEIQEKIADILFAFEKLCNDLVEGIPAEVEMRKKQLDYYQNFLFN", "aa_hexdigest": "e7efe66ecfd5fa96653adf12d03ce3d8", "start_type": "ATG", "rbs_motif": "AAAA", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 436424, "stop": 436756, "observations": { "mutation": [ 436736 ], "stop": [ 436736 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00190F3D87", "query_cov": 1.0, "subject_cov": 0.46218487394957986, "identity": 0.9640000000000001, "score": 112.0, "evalue": 6.15e-31, "gene_start": 1, "gene_end": 55, "reference_start": 50, "reference_end": 104, "reference_sequence": "GKCNASQDCGVLKVKNKKICTKFLSFLLKIEAPKFVHNLASRPKLSQKVMAEIELSFPPLEIQEKIADILFAFEKLCNDLVEGIPAEIELRKKQLDYYQNFLFNWVQEQKKNSLSTNLN" }, "paralog": false, "identity": 0.9636363636363636, "score": 213.0, "evalue": 6.53e-73, "description": "Internal stop codon at 436736. Nonsense mutation around 436736.", "psc": { "uniref90_id": "UniRef90_UPI00190F3D87", "product": "restriction endonuclease subunit S", "ec_ids": [ "3.1.21.3" ], "uniref50_id": "UniRef50_UPI00190F3D87", "kegg_orthology_id": "K01154", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00190F3D87", "EC:3.1.21.3", "UniRef:UniRef50_UPI00190F3D87", "KEGG:K01154" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00190F3D87", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00190F3D87" ], "product": "restriction endonuclease subunit S" } }, "id": "APKECPOBEN_793", "locus": "GCA002090215_00769" }, { "type": "cds", "sequence": "contig_1", "start": 436930, "stop": 437490, "strand": "+", "frame": 1, "gene": null, "product": "Putative MgpC-like protein MPN_366", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75415", "UniRef:UniRef90_P75415" ], "nt": "GTGGGACAGAACAAGTTAGTGGCGCTGGTCCGCTCAGCGTTGGTCAGGGTGAAATTCCAGGACACAACGAGTTCGGACTCTAATAACCAAGATACGAGTCAAAATGCCTTGAGTTTTGATACCCAAGAATCACAGAAGGCACTTAATGGCTCGCAGAGTGGATCTTCTGACACTTCCGGGTCTAACTCCCAAGACTTCGCCAGCTACATCCTCATCTTCCAAGCCGCGCCCAGGGCCACGTGGGTGTTTGAACGGAAGATTAAGTTGGCGTTGCCCTACGTTAAGCAGGAAAGTCAGGGTTCCGACGATCAAGGTTCCAATGGTAAGGGCTCCCTCTACACCACCCTCCAAGACCTCCTCGTCGAACAACCCGTGACCCCTTATACGCCCAATGCGGGGTTAGCCCGGGTGAATGGGGTTGCTCAGGATACGGTTCATTTTGGTTCGGGTCAAGAATCGAGTTGGAATTCCCAACGTTCCCAAAAAGGCCTTAAAACAACCCCGCTCCCAATGCCGTCACCGGCTTTAAGCTCGATAAGGGCCGCGCGTACCGGAAGCTGA", "aa": "MGQNKLVALVRSALVRVKFQDTTSSDSNNQDTSQNALSFDTQESQKALNGSQSGSSDTSGSNSQDFASYILIFQAAPRATWVFERKIKLALPYVKQESQGSDDQGSNGKGSLYTTLQDLLVEQPVTPYTPNAGLARVNGVAQDTVHFGSGQESSWNSQRSQKGLKTTPLPMPSPALSSIRAARTGS", "aa_hexdigest": "e9299a9d27a3c322ea824d325f98707b", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 436861, "stop": 437679, "observations": { "mutation": [ 436921, 437488 ], "stop": [ 436921, 437488 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75415", "query_cov": 1.0, "subject_cov": 0.6838235294117647, "identity": 0.995, "score": 349.0, "evalue": 3.31e-120, "gene_start": 1, "gene_end": 186, "reference_start": 24, "reference_end": 209, "reference_sequence": "MGGLDVVRAAHLHPSYELVDWKRVGQNKLVALVRSALVRVKFQDTTSSDSNNQDTSQNALSFDTQESQKALNGSQSGSSDTSGSNSQDFASYILIFQAAPRATWVFERKIKLALPYVKQESQGSDDQGSNGKGSLYTTLQDLLVEQPVTPYTPNAGLARVNGVAQDTVHFGSGQESSWNSQRSQKGLKTTPLPMPSPALSSIRAARTGSWMKVDRCMNPWIRPRRGRGRMRALGKIRKKQRRKMMPRWWGWLEVVRLEVLLVYKAMARTVRG" }, "paralog": false, "identity": 0.9926470588235294, "score": 536.0, "evalue": 4.54e-196, "description": "Internal stop codon at 436921, 437488. Nonsense mutation around 436921, 437488.", "psc": { "uniref90_id": "UniRef90_P75415", "product": "Putative MgpC-like protein MPN_366", "uniref50_id": "UniRef50_P75415", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75415", "UniRef:UniRef50_P75415" ] }, "pscc": { "uniref50_id": "UniRef50_P75415", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75415" ], "product": "Putative MgpC-like protein MPN_366" } }, "id": "APKECPOBEN_794", "locus": "GCA002090215_00770" }, { "type": "cds", "sequence": "contig_1", "start": 437505, "stop": 437921, "strand": "+", "frame": 3, "gene": "mgpC", "product": "MgpC family cytadherence protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75414", "UniRef:UniRef90_UPI001F33AF27" ], "nt": "GTGTATGAACCCCTGGATTCGACCAAGGAGGGGAAGGGGAAGGATGAGAGCTCTTGGAAAAATTCGGAAAAAACAACGGCGGAAAATGATGCCCCGTTGGTGGGGATGGTTGGAAGTGGTGCGGCTGGAAGTGCTTCTAGTTTACAAGGCAATGGCTCGAACAGTTCGGGGTTAAAATCGCTCTTGAGATCAGCACCTGTCAGTGTTCCACCAAGCAGTACAAGTAATCAAACTTTAAGCTTATCTAACCCCGCTCCTGTAGGCCCACAAGCGGTTGTAAGCCAACCCGCGGGGGGTGCTACGGCAGCAGTGTCCGTCAATCGCACAGCGAGTGACACCGCCACCTTTAGCAAGTACCTCAACACCGCCCAGGCCTTGCACCAGATGGGGGTGATTGTTCCGGGGTTGGAAAAATGA", "aa": "MYEPLDSTKEGKGKDESSWKNSEKTTAENDAPLVGMVGSGAAGSASSLQGNGSNSSGLKSLLRSAPVSVPPSSTSNQTLSLSNPAPVGPQAVVSQPAGGATAAVSVNRTASDTATFSKYLNTAQALHQMGVIVPGLEK", "aa_hexdigest": "8843f90dd8ff213ac750e6c3d8273e11", "start_type": "GTG", "rbs_motif": null, "genes": [ "mgpC" ], "truncated": "3-prime", "pseudogene": { "start": 437505, "stop": 438224, "observations": { "mutation": [ 437919 ], "stop": [ 437919 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001F33AF27", "query_cov": 1.0, "subject_cov": 0.4726027397260274, "identity": 0.993, "score": 246.0, "evalue": 7.28e-80, "gene_start": 1, "gene_end": 138, "reference_start": 1, "reference_end": 138, "reference_sequence": "MYEPLDSTKEGKGKDESSWKNSEKTTAENDAPLVGMVGSGAAGSASSLQGNGSNSSGLKSLLRSAPVSVPPSSTSNQTLSLSNPAPVGPQAVVSQPAGGATAAVSVNRTASDTSTFSKYLNTAQALHQMGVIVPGLEKWGGNNGTGVVASRRDATSTNLPHAAGASQTGLGTGSPREPALTATSQRAVTVVAGPLRAGNSSETDALPNVITQLYHTSTAQLAYLNGQIVVMGSDRVPSLWYWVVGEDQESGKATWWAKTELNWGTDKQKQFVENQLGFKDDSNSDSKNSNLK" }, "paralog": false, "identity": 0.9832635983263598, "score": 455.0, "evalue": 9.67e-165, "description": "Internal stop codon at 437919. Nonsense mutation around 437919.", "psc": { "uniref90_id": "UniRef90_UPI001F33AF27", "product": "MgpC family cytadherence protein", "uniref50_id": "UniRef50_P75414", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001F33AF27", "UniRef:UniRef50_P75414" ] }, "pscc": { "uniref50_id": "UniRef50_P75414", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75414" ], "product": "Putative MgpC-like protein MPN_367" } }, "id": "APKECPOBEN_795", "locus": "GCA002090215_00771" }, { "type": "cds", "sequence": "contig_1", "start": 437946, "stop": 438230, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGCTAGCCGACGGGATGCTACTTCCACTAACCTGCCCCATGCGGCAGGTGCTTCCCAAACGGGTTTGGGAACTGGTTCGCCCCGCGAACCAGCTTTAACCGCAACGTCACAGCGTGCCGTCACGGTGGTTGCTGGCCCCCTTCGTGCGGGCAATAGCAGTGAAACTGATGCCCTACCGAATGTCATCACCCAGCTCTATCATACTTCAACCGCCCAACTCGCTTACTTAAATGGCCAGATCGTTGTGATGAGTTCCGACCGGGTACCGAGTCTTTGGTATTGA", "aa": "MASRRDATSTNLPHAAGASQTGLGTGSPREPALTATSQRAVTVVAGPLRAGNSSETDALPNVITQLYHTSTAQLAYLNGQIVVMSSDRVPSLWY", "aa_hexdigest": "61b1b8708f9c1933f5ec833cf3e65ebf", "start_type": "GTG", "rbs_motif": "AACAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9782.783, "isoelectric_point": 8.291746330261233 }, "id": "APKECPOBEN_796", "locus": "GCA002090215_00772" }, { "type": "cds", "sequence": "contig_1", "start": 438330, "stop": 438578, "strand": "+", "frame": 3, "gene": "mgpC", "product": "MgpC family cytadherence protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB33HQG9", "UniRef:UniRef90_UPI0023EE2DD8" ], "nt": "TTGGGGTTTAAAGATGACTCAAATTCGGATTCCAAAAATTCGAATTTGAAGACCCAAGGCCTCACCCAACCCGCCTACCTCATCGCCGGTCTTGACGTTGTGGCCGACCACCTCGTCTTTGCGGCATTTAAAGCGGGCGCGGTGGGGTATGATATGACGACTGATTCGAACGCTTCGACCTACAACCAAGCACTCGTCTGGTCGACCACGGCCGGGTTGGACAGTGATGGGGGGACAAGGCTTTGGTAG", "aa": "MGFKDDSNSDSKNSNLKTQGLTQPAYLIAGLDVVADHLVFAAFKAGAVGYDMTTDSNASTYNQALVWSTTAGLDSDGGTRLW", "aa_hexdigest": "6a8dd1b2ae3b904bb6f4420edaceaf01", "start_type": "TTG", "rbs_motif": null, "genes": [ "mgpC" ], "truncated": "both", "pseudogene": { "start": 438195, "stop": 438718, "observations": { "deletion": [ 438567 ], "mutation": [ 438228, 438267, 438270 ], "stop": [ 438228, 438267, 438270 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0023EE2DD8", "query_cov": 0.9512195121951219, "subject_cov": 0.43575418994413406, "identity": 0.9490000000000001, "score": 148.0, "evalue": 8.15e-44, "gene_start": 1, "gene_end": 78, "reference_start": 46, "reference_end": 123, "reference_sequence": "MGSDRVPSLWYWVVGEDQESGKATWWAKTELNWGTDKQKQFVENQLGFKDDSNSDSKNSNLKAQGLTQPAYLIAGLDVVADHLVFAAFKAGAVGYDMTTDSSASTYNQALAWSTTAGLDSDGGYKALVENTAGLNGPINGLFTLLDTFAYVTPVSGMKGGSQNNEEVQTTYPVERGESE" }, "paralog": false, "identity": 0.9482758620689655, "score": 324.0, "evalue": 1.58e-115, "description": "Internal stop codon at 438228, 438267, 438270. Frameshift due to deletion around 438567. Nonsense mutation around 438228, 438267, 438270.", "psc": { "uniref90_id": "UniRef90_UPI0023EE2DD8", "product": "MgpC family cytadherence protein", "uniref50_id": "UniRef50_A0AB33HQG9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0023EE2DD8", "UniRef:UniRef50_A0AB33HQG9" ] }, "pscc": { "uniref50_id": "UniRef50_A0AB33HQG9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB33HQG9" ], "product": "Cytadherence-related protein ORF6 homolog" } }, "id": "APKECPOBEN_797", "locus": "GCA002090215_00773" }, { "type": "cds", "sequence": "contig_1", "start": 438572, "stop": 438874, "strand": "+", "frame": 2, "gene": "mgpC", "product": "MgpC protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W8G0", "UniRef:UniRef90_A0AB38W8G0" ], "nt": "TTGGTAGAAAACACGGCCGGGCTCAACGGCCCGATTAATGGTTTGTTTACCCTGCTCGACACCTTTGCGTATGTGACCCCCGTGAGTGGGATGAAAGGGGGGAGTCAGAATAATGAAGAAGTGCAAACGACTTACCCGGTCAAGTCCGACCAAAAGGCCACCGCCAAAATTGCCTCCTTAATTAATGCCAGCCCACTCAACAGTTATGGGGATGATGGCGATAAAAAAGCCATCTACCCAGAGGTAAGAGGCAGACAGCTCCAAAATATTTTTGACGATTGGAAAACTAACAAAAGCTGTTAG", "aa": "MVENTAGLNGPINGLFTLLDTFAYVTPVSGMKGGSQNNEEVQTTYPVKSDQKATAKIASLINASPLNSYGDDGDKKAIYPEVRGRQLQNIFDDWKTNKSC", "aa_hexdigest": "8ecca72c76c9948b21b33a8ec53cae0a", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0AB38W8G0", "query_cov": 1.0, "subject_cov": 0.8695652173913043, "identity": 0.99, "score": 207.0, "evalue": 5.61e-68, "valid": true, "product": "MgpC protein", "uniref50_id": "UniRef50_A0AB38W8G0" }, "pscc": { "uniref50_id": "UniRef50_A0AB38W8G0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W8G0" ], "product": "MgpC protein" }, "genes": [ "mgpC" ], "id": "APKECPOBEN_798", "locus": "GCA002090215_00774" }, { "type": "cds", "sequence": "contig_1", "start": 438895, "stop": 439038, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W8B4", "UniRef:UniRef90_A0AB38W8B4" ], "nt": "ATGAATTTACTAAATTTATTTAAATCAGCGGGAGCAGGCTCGTTTTTTCAACAAAAGTCACTCTATCTGCTCCCACTAAATAAATTGACAGAGTTTTATAAAAAAGCCAAAGAGTTTGAATATTTTTGTAATTTGGGTTTTTAA", "aa": "MNLLNLFKSAGAGSFFQQKSLYLLPLNKLTEFYKKAKEFEYFCNLGF", "aa_hexdigest": "086385bb634148ef1c83df39d50e5df5", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 438895, "stop": 439074, "observations": { "mutation": [ 439036 ], "stop": [ 439036 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB38W8B4", "query_cov": 0.9787234042553191, "subject_cov": 0.6388888888888888, "identity": 0.87, "score": 83.6, "evalue": 3.14e-20, "gene_start": 1, "gene_end": 46, "reference_start": 1, "reference_end": 46, "reference_sequence": "MNLLNLFKSAGAGSFFQHKSLYLLPLNKLRQIFKVAKEFEYFCNLGLQLHLTISRVFTFENFFGLILAPRLF" }, "paralog": false, "identity": 0.7627118644067796, "score": 92.0, "evalue": 2.52e-26, "description": "Internal stop codon at 439036. Nonsense mutation around 439036.", "psc": { "uniref90_id": "UniRef90_A0AB38W8B4", "uniref50_id": "UniRef50_A0AB38W8B4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB38W8B4", "UniRef:UniRef50_A0AB38W8B4" ] }, "pscc": { "uniref50_id": "UniRef50_A0AB38W8B4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W8B4" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 5503.392, "isoelectric_point": 9.252131843566893 }, "id": "APKECPOBEN_799", "locus": "GCA002090215_00775" }, { "type": "cds", "sequence": "contig_1", "start": 439435, "stop": 439809, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF16526.10", "PFAM:PF01519.22", "PFAM:PF03670.18" ], "nt": "GTGAACACAGAATACGTAACGCGTAAAGAGTTCAACGAGTACAAAGACTCAAACGACCAACGACTTACAAAGATTGAAAATAAGGTCGATAAGCTTGAAATTAAAGTTGATAAGCTAGAGAAGAAAGTCGATAAGCTGGAAGTTAAGGTTGATAAACTAGTCGAAACTGTTAATGCTCAAGGAGAAGATCTTAACAACTTCAAAGTTGAAGTGAGAGGAACACTTCAAAGCCAAGGCGAAACCCTGCAATTAATTTTGCAAACGCTACAAGGCATGAGTAAGCGGCTTGATTCTGTTGAAGGACGTTTAGACTCTATGGATGGTCGTCTCGATTCAATGGAAACTCGTTTGGACAAAATCGATCCGCTAAAATAG", "aa": "MNTEYVTRKEFNEYKDSNDQRLTKIENKVDKLEIKVDKLEKKVDKLEVKVDKLVETVNAQGEDLNNFKVEVRGTLQSQGETLQLILQTLQGMSKRLDSVEGRLDSMDGRLDSMETRLDKIDPLK", "aa_hexdigest": "aaeb409c7e331d3a9e52da54cbb9a5ba", "start_type": "GTG", "rbs_motif": "AACAA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF16526.10", "name": "CLZ", "length": 46, "aa_cov": 0.3709677419354839, "hmm_cov": 1.0, "evalue": 2.9172697186121933e-08, "score": 29.50621795654297, "start": 19, "stop": 64 }, { "id": "PF01519.22", "name": "DUF16", "length": 100, "aa_cov": 0.8064516129032258, "hmm_cov": 0.89, "evalue": 8.434234507998811e-35, "score": 114.91099548339844, "start": 2, "stop": 101 }, { "id": "PF03670.18", "name": "UPF0184", "length": 45, "aa_cov": 0.3629032258064516, "hmm_cov": 1.0, "evalue": 4.0919532839417793e-07, "score": 25.839595794677734, "start": 12, "stop": 56 } ], "seq_stats": { "molecular_weight": 14373.17969999999, "isoelectric_point": 5.170950126647948 }, "id": "APKECPOBEN_800", "locus": "GCA002090215_00776" }, { "type": "cds", "sequence": "contig_1", "start": 439941, "stop": 440033, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAACAAATTTTTAAGTACCTTTGCTAATAATACAGAAGGCTATTCATCAGATATTAATCTCTTCATTTACTTGGAATATTTAATTAAGTAA", "aa": "MNKFLSTFANNTEGYSSDINLFIYLEYLIK", "aa_hexdigest": "b0e26d257c1f12e45212b995b9cda380", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3549.9948000000013, "isoelectric_point": 4.677076148986816 }, "id": "APKECPOBEN_801", "locus": "GCA002090215_00777" }, { "type": "cds", "sequence": "contig_1", "start": 440097, "stop": 440366, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAGCGAAAGATTGAATATTTCCAGATGACATACCAAAGTTTTAAGGATTTGTCAATTGAAGCTAAGTTGAGCTATACCTTTAATTGGTTTGGAGATTATTCTTCAGGTGATTTCACTGCTAAGAAAGGTGACAAGCATTATTTTGATCTTTTCCTTAAAATTAAATCTAATTCTGATCCAGGAAAACAATTTACTACAGAAAAATTTCTTACTAAAGAAGAAAAAATTAAAGTTAATGGAAAAGAAATTACAAGAAATTTAGAGTGA", "aa": "MERKIEYFQMTYQSFKDLSIEAKLSYTFNWFGDYSSGDFTAKKGDKHYFDLFLKIKSNSDPGKQFTTEKFLTKEEKIKVNGKEITRNLE", "aa_hexdigest": "cfdf950205690ee16424a3112fa284e2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10600.865199999991, "isoelectric_point": 8.753146553039553 }, "id": "APKECPOBEN_802", "locus": "GCA002090215_00778" }, { "type": "cds", "sequence": "contig_1", "start": 440454, "stop": 440702, "strand": "-", "frame": 2, "gene": null, "product": "putative lipoprotein MPN_467", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75316" ], "nt": "ATGCGCAAAAAGAAGTTTTTGTCAAAGGTTTCCTTTGGTTCTTTATTTTTGTTATGTGGAACAGTTTTAAGCGCCTGTACTGGTATCCAAGCAGATCTTAGAAATCTTATTAAAGAAGCCACCGGTAAAGACATTGATGTGTATAAAGCAATTAAAACTACAGAAGGCAAAAAGAACATGATAACTTCTCTAAAGAAGTCTTACAAAGTTAATCCGAAAGATACAACAAAATTGTTGTTAGACGCCTGA", "aa": "MRKKKFLSKVSFGSLFLLCGTVLSACTGIQADLRNLIKEATGKDIDVYKAIKTTEGKKNMITSLKKSYKVNPKDTTKLLLDA", "aa_hexdigest": "9a74f050e0e7a42e1d591c8b79760ceb", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75316", "query_cov": 1.0, "subject_cov": 0.8118811881188119, "identity": 0.8290000000000001, "score": 134.0, "evalue": 1.63e-39, "valid": false, "product": "Uncharacterized lipoprotein MPN_467", "uniref50_id": "UniRef50_P75316", "go_ids": [ "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75316", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75316" ], "product": "Uncharacterized lipoprotein MPN_467" }, "genes": [], "id": "APKECPOBEN_803", "locus": "GCA002090215_00779" }, { "type": "cds", "sequence": "contig_1", "start": 441172, "stop": 441291, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0H3DJX7", "UniRef:UniRef90_A0AAN4XEA6" ], "nt": "ATGGTAGCTTACTTTGATCCAAAGGAAGACGGTACTTGTGAATTCATTATGAAAATTAAAACTGTAAATGAAGTTGCTGACACTGAGATAACAAATAAGGAAAAGCCTTTAGGCACTTGA", "aa": "MVAYFDPKEDGTCEFIMKIKTVNEVADTEITNKEKPLGT", "aa_hexdigest": "230972b44fd8b8153b632bd9c81fe607", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 441070, "stop": 441300, "observations": { "mutation": [ 441151, 441289 ], "stop": [ 441151, 441289 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAN4XEA6", "query_cov": 1.0, "subject_cov": 0.5131578947368421, "identity": 1.0, "score": 83.2, "evalue": 3.49e-20, "gene_start": 1, "gene_end": 39, "reference_start": 35, "reference_end": 73, "reference_sequence": "EKRKEHLNMKKVKALRNLFLEKASEKLEYPKIASMVAYFDPKEDGTCEFIMKIKTVNEVADTEITNKEKPLGTWKS" }, "paralog": false, "identity": 0.9605263157894737, "score": 145.0, "evalue": 3.27e-47, "description": "Internal stop codon at 441151, 441289. Nonsense mutation around 441151, 441289.", "psc": { "uniref90_id": "UniRef90_A0AAN4XEA6", "uniref50_id": "UniRef50_A0A0H3DJX7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAN4XEA6", "UniRef:UniRef50_A0A0H3DJX7" ] }, "pscc": { "uniref50_id": "UniRef50_A0A0H3DJX7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0H3DJX7" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 4407.0016000000005, "isoelectric_point": 4.554986000061036 }, "id": "APKECPOBEN_804", "locus": "GCA002090215_00780" }, { "type": "cds", "sequence": "contig_1", "start": 441343, "stop": 441675, "strand": "+", "frame": 1, "gene": null, "product": "Adhesin P1-like protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB33HQB0", "UniRef:UniRef90_A0AB33HQB0" ], "nt": "GTGTTAAAACTTGCTGTTGGTATTTTTATTTCCCCCACGCTCACCAGGTTTAGTACCGGGTTCAACCTCGCGGGGTCGGTGCTCGACCAGGTGTTGGATTATGTGCCCTGGATTGGGAATGGGCACAGGTATGGGAATAACCACCGGGGCGTGGATGATATAACCGCGCCTAAAACCGGCGCGGGGTCGTCCAGCGGAACTAGTACGAACACAAGTGGTTCGCGTTCCTTTCTCCCGACGTTCTCGAATGTCGGCGTTGGCCTCAAAGCGAATGTCCAGGGCACCCTGGGTGGCAGTCAGACGACGACTACGGGGAAAGATATTCCCAAATGA", "aa": "MLKLAVGIFISPTLTRFSTGFNLAGSVLDQVLDYVPWIGNGHRYGNNHRGVDDITAPKTGAGSSSGTSTNTSGSRSFLPTFSNVGVGLKANVQGTLGGSQTTTTGKDIPK", "aa_hexdigest": "f4c9baa25489f2b1c2371bc847c3419b", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 441343, "stop": 441976, "observations": { "deletion": [ 441949, 441952 ], "mutation": [ 441673, 441706, 441721 ], "stop": [ 441673, 441706, 441721 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB33HQB0", "query_cov": 1.0, "subject_cov": 0.41509433962264153, "identity": 1.0, "score": 216.0, "evalue": 3.39e-69, "gene_start": 1, "gene_end": 110, "reference_start": 1, "reference_end": 110, "reference_sequence": "MLKLAVGIFISPTLTRFSTGFNLAGSVLDQVLDYVPWIGNGHRYGNNHRGVDDITAPKTGAGSSSGTSTNTSGSRSFLPTFSNVGVGLKANVQGTLGGSQTTTTGKDIPKWPTLDPAKLQLWTGAGWRNDKASNKQSDENHTTFKSATGSGQQGGSTTGGSAGNPDSLKQDKISKSGQNLTTQDGAPQSNSTTESASNYDHLPPPTSPPHPIDRTRCHSPTRTTRSAPSSSSAACWAASRCWWIEVGQMIPTNSKPPTKNGLTPT" }, "paralog": false, "identity": 0.976303317535545, "score": 404.0, "evalue": 1.52e-145, "description": "Internal stop codon at 441673, 441706, 441721. Frameshift due to deletion around 441949, 441952. Nonsense mutation around 441673, 441706, 441721.", "psc": { "uniref90_id": "UniRef90_A0AB33HQB0", "product": "Adhesin P1 homolog", "uniref50_id": "UniRef50_A0AB33HQB0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB33HQB0", "UniRef:UniRef50_A0AB33HQB0" ] }, "pscc": { "uniref50_id": "UniRef50_A0AB33HQB0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB33HQB0" ], "product": "Adhesin P1 homolog" } }, "id": "APKECPOBEN_805", "locus": "GCA002090215_00781" }, { "type": "cds", "sequence": "contig_1", "start": 442060, "stop": 442341, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTGGTGAATCGAAGTGGGTCAGATGATTCCAACAAATTCCAAGCCACCGACCAAAAATGGTCTTACACCGACTTAAAGTCGGACCAAACCAAGCTCAACCTCCCCGCTTATGGTGAAGTGAATGGGTTGTTGAATCCGGCGTTGGTGGAAACCTATTTTGGGACGACGCGAGCGGGTGGTTCGGGGTCCAACACGACCAGTTCACCCGGTATCGGTTTTAAAATTCCCGAACAAAATAATGATTCGAAGGCTGTGCTGATCACCCCCGGGTTGGCTTGA", "aa": "MLVNRSGSDDSNKFQATDQKWSYTDLKSDQTKLNLPAYGEVNGLLNPALVETYFGTTRAGGSGSNTTSSPGIGFKIPEQNNDSKAVLITPGLA", "aa_hexdigest": "2649d38c15df25edcb4701e922358f22", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9868.75109999999, "isoelectric_point": 5.125819969177246 }, "id": "APKECPOBEN_806", "locus": "GCA002090215_00782" }, { "type": "cds", "sequence": "contig_1", "start": 442836, "stop": 443138, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_054173230.1", "SO:0001217", "UniParc:UPI0002D6A4F6", "UniRef:UniRef100_UPI0002D6A4F6", "UniRef:UniRef50_UPI000364214F", "UniRef:UniRef90_UPI000364214F", "PFAM:PF03257.18" ], "nt": "TTGGACCCCAACCAGGTTCGCACCAAGCTGCGCCAAAGCTTTGGTACAGACCATTCCACCCAAGCCAAACCCCAATCCCTCAAAACAACCACACCGGTGTTTGGGACGAATAGTGGTAACATTGGCAGTGTGCTTAGTGGTGGGGGTGCTGGAGGAGCAGACAGCACCAATTCGGTGGACCTCTCCCCCGTTGAACGGGTGAGTGGGTGGCTTGTGGGGCAATTACCCAGTGGGGGTGGGGGGAATAGTAGTGAGGATATTAAAAGTGTGCAAGACACTCCTATTTATATCTATATTCATTAG", "aa": "MDPNQVRTKLRQSFGTDHSTQAKPQSLKTTTPVFGTNSGNIGSVLSGGGAGGADSTNSVDLSPVERVSGWLVGQLPSGGGGNSSEDIKSVQDTPIYIYIH", "aa_hexdigest": "91ea1f2ade9a683442b9961b2d3fc062", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0002D6A4F6", "ncbi_nrp_id": "WP_054173230.1", "uniref100_id": "UniRef100_UPI0002D6A4F6", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002D6A4F6", "RefSeq:WP_054173230.1", "UniRef:UniRef100_UPI0002D6A4F6" ] }, "ips": { "uniref100_id": "UniRef100_UPI0002D6A4F6", "uniref90_id": "UniRef90_UPI000364214F" }, "psc": { "uniref90_id": "UniRef90_UPI000364214F", "uniref50_id": "UniRef50_UPI000364214F" }, "pscc": { "uniref50_id": "UniRef50_UPI000364214F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000364214F" ] }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03257.18", "name": "Adhesin_P1_C", "length": 71, "aa_cov": 0.7, "hmm_cov": 1.0, "evalue": 5.64608961448071e-27, "score": 90.00322723388672, "start": 1, "stop": 70 } ], "seq_stats": { "molecular_weight": 10318.173999999999, "isoelectric_point": 6.006426429748534 }, "id": "APKECPOBEN_807", "locus": "GCA002090215_00783" }, { "type": "cds", "sequence": "contig_1", "start": 443395, "stop": 443556, "strand": "+", "frame": 1, "gene": null, "product": "Cytadhesin P1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W6U4", "UniRef:UniRef90_A0AB38W6U4" ], "nt": "GTGCGCGACAACACCGCCAAGGGCATCACTGCCGGCAGTGGCAGTCAACAAACCACGTATGATCCCACCCGAACCGAAGCGGCTTTGACCACCGCCACCACCTTTGTGTTACGCCGATACGACCTCGCCGGGCGTTGTACGACCTCGACTTTTCGAAGTTAA", "aa": "MRDNTAKGITAGSGSQQTTYDPTRTEAALTTATTFVLRRYDLAGRCTTSTFRS", "aa_hexdigest": "b72f89e961c0b2be32e348aa25fbe6db", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 443212, "stop": 443556, "observations": { "mutation": [ 443326 ], "stop": [ 443326 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB38W6U4", "query_cov": 1.0, "subject_cov": 0.4649122807017544, "identity": 0.9620000000000001, "score": 99.8, "evalue": 5.16e-26, "gene_start": 1, "gene_end": 53, "reference_start": 62, "reference_end": 114, "reference_sequence": "MGHFTSTTTTLKRQQFSYTRPDEVALRHTNAINPRLTPWTYRNTSFSSLPLTGENPGAWALVRDNTAKGITAGSGSQQTTYDPTRTEAALTTATTFALRRYDLAGRCTTSTFRS" }, "paralog": false, "identity": 0.9736842105263158, "score": 226.0, "evalue": 1.77e-78, "description": "Internal stop codon at 443326. Nonsense mutation around 443326.", "psc": { "uniref90_id": "UniRef90_A0AB38W6U4", "product": "Cytadhesin P1", "uniref50_id": "UniRef50_A0AB38W6U4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB38W6U4", "UniRef:UniRef50_A0AB38W6U4" ] }, "pscc": { "uniref50_id": "UniRef50_A0AB38W6U4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W6U4" ], "product": "Cytadhesin P1" } }, "id": "APKECPOBEN_808", "locus": "GCA002090215_00784" }, { "type": "cds", "sequence": "contig_1", "start": 443679, "stop": 443990, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGCGCAAGACCCCTCCAATCCCTACCGGTTTGCCGTTTTACTCGTGCCGCGCAGCGTGGTGTACTATGAGCAGTTGCAAAGGGGGTTGGGCTTACCACAGCAGCGAACCGAGAATGGTCAAAATACTTCCACCACCGGGGCAATGTTTGGCTTGAAGGTGAAGAACGCCGAGGCGGACACCGCGAAGAGCAATGAAAAACTCCAGGGCGCTGAGGCCACTGGTTCTTCAACCACATCTGGATCTGGCCAATCCACCCAACGTGGGGTTCGTCAGGGGACACCAAAGTCAAGGCGTTGCAGGTGGCGGTGA", "aa": "MAQDPSNPYRFAVLLVPRSVVYYEQLQRGLGLPQQRTENGQNTSTTGAMFGLKVKNAEADTAKSNEKLQGAEATGSSTTSGSGQSTQRGVRQGTPKSRRCRWR", "aa_hexdigest": "4a8e3336df3f774fb1b6bb0024556791", "start_type": "GTG", "rbs_motif": "AACAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11234.360299999993, "isoelectric_point": 10.416430854797362 }, "id": "APKECPOBEN_809", "locus": "GCA002090215_00785" }, { "type": "cds", "sequence": "contig_1", "start": 443981, "stop": 444169, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGCGGTGAAAAAGAAATCGGACTCGGAGGACAATGGTCAGATCGAACTTGAAACCAACAACCTCGCCAACGCCCCGATTAAACGGGGCTCCAATAACAACCAGCAAGTCCAACTCAAGGCGGACGATTTTGGTACTGCCCCTTCCAGTTCGGGATCAGGCACCCAAGATGGCACCCCCACCCCCTGA", "aa": "MAVKKKSDSEDNGQIELETNNLANAPIKRGSNNNQQVQLKADDFGTAPSSSGSGTQDGTPTP", "aa_hexdigest": "b5dd8aec831b855a2d7bab32d23ae3b8", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6460.844200000005, "isoelectric_point": 4.716181373596191 }, "id": "APKECPOBEN_810", "locus": "GCA002090215_00786" }, { "type": "cds", "sequence": "contig_1", "start": 444388, "stop": 444831, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02917.20", "PFAM:PF22596.2" ], "nt": "ATGCCAAATCCTGTTAGATTTGTTTACCGTGTTGATTTGAGAAGCCCTGAAGAAATTTTTGAACATGGCTTTTCAACTTTAGGTGATGTGAGAAATTTCTTTGAACACATTCTCTCCACTAATTTTGGTAGAAGCTATTTTATTTCCACTTCAGAAACACCCACAGCAGCTATTCGCTTCTTTGGTAGCTGGTTACGGGAATATGTACCAGAGCACCCCAGAAGGGCTTACTTATATGAAATTCGTGCCGACCAACACTTTTACAATGCCCGCGCCACTGGGGAGAACTTGTTAGATTTAATGCGTCAAAGACAAGTAGTATTTGACTCTGGTGATCGAGAAATGGCACAAATGGGAATTAGAGCTTTACGCACTTCCTTTGCGTATCAACGTGAATGGTTTACCGATGGTCCAATTGCAGCAGCTAATGTCCGTAGTGCTTGA", "aa": "MPNPVRFVYRVDLRSPEEIFEHGFSTLGDVRNFFEHILSTNFGRSYFISTSETPTAAIRFFGSWLREYVPEHPRRAYLYEIRADQHFYNARATGENLLDLMRQRQVVFDSGDREMAQMGIRALRTSFAYQREWFTDGPIAAANVRSA", "aa_hexdigest": "991ddd04da404e2e6271f5eeddd807ba", "start_type": "ATG", "rbs_motif": "AAAA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02917.20", "name": "Pertussis_S1", "length": 146, "aa_cov": 0.9727891156462585, "hmm_cov": 1.0, "evalue": 2.5829564962724187e-83, "score": 274.9464111328125, "start": 3, "stop": 145 }, { "id": "PF22596.2", "name": "Scabin-like", "length": 114, "aa_cov": 0.7278911564625851, "hmm_cov": 0.9298245614035088, "evalue": 4.5296986963247205e-08, "score": 28.53148651123047, "start": 8, "stop": 114 } ], "seq_stats": { "molecular_weight": 17225.12139999999, "isoelectric_point": 6.919317436218263 }, "id": "APKECPOBEN_811", "locus": "GCA002090215_00787" }, { "type": "cds", "sequence": "contig_1", "start": 445246, "stop": 445479, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF22509.2" ], "nt": "ATGTTACAAGCAGATCCGCAAAATAACAATGTCTTTTTGGTCGAAGTAAACCCCAAACAAAAGTCCAGCTTTCCCCAAACCATCTTCTTTTGGGATGTTTATCAACGAATTTGTCTCAAGGATTTAACTGGTGCACAAATCAGTCTTTCGCTTACTGCCTTTACTACTCAGTATGCTGGTCAGCTCAAAGTGCACCTTAGTGTTAGCGCGGTTAATGCCGTGAACCAAAAGTGA", "aa": "MLQADPQNNNVFLVEVNPKQKSSFPQTIFFWDVYQRICLKDLTGAQISLSLTAFTTQYAGQLKVHLSVSAVNAVNQK", "aa_hexdigest": "6150e794e3b170ad6ba22a8f853ffe68", "start_type": "ATG", "rbs_motif": "AAAA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF22509.2", "name": "CARDS-D2_trefoil", "length": 84, "aa_cov": 1.0, "hmm_cov": 1.0, "evalue": 5.936217380437595e-38, "score": 125.75577545166016, "start": 1, "stop": 77 } ], "seq_stats": { "molecular_weight": 8644.801099999999, "isoelectric_point": 8.931723976135256 }, "id": "APKECPOBEN_812", "locus": "GCA002090215_00788" }, { "type": "cds", "sequence": "contig_1", "start": 445783, "stop": 445911, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF22346.2" ], "nt": "ATGAAAACACCAGATGGACAGATATTCTATGATCTAAAAACTTCGAAAATCTTCTTTGTCCAGGACAACCAAAACGTGTTCTTTCTCCATAATAAACTCAACAAACAAACTGGTTACAGCTGGGATTGA", "aa": "MKTPDGQIFYDLKTSKIFFVQDNQNVFFLHNKLNKQTGYSWD", "aa_hexdigest": "04c19e688bad2cd46fd20e5ed0ad5150", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF22346.2", "name": "CARDS_D3", "length": 42, "aa_cov": 1.0, "hmm_cov": 0.9761904761904762, "evalue": 1.0199585584754571e-17, "score": 59.96404266357422, "start": 1, "stop": 42 } ], "seq_stats": { "molecular_weight": 5057.648300000002, "isoelectric_point": 8.085834312438966 }, "id": "APKECPOBEN_813", "locus": "GCA002090215_00789" }, { "type": "cds", "sequence": "contig_1", "start": 445933, "stop": 446163, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF22346.2" ], "nt": "ATGAATGAGGACAAAGACGAAAACTTTAAATGGTACTTTTCGCGTGATGACCTTACCATTCCTTCCGTTGAAGGGCTTAACTTCCGCCACATTCGCTGTTACGCTGACAACCAGCAGTTAAAGGTGATCATAAGCGGTTCACGTTGGGGCGGTTGGTACTCCACTTACGATAAAGTTGAAAGTAATGTCGAAGATAAGATTTTGGTCAAAGATGGTTTTGATCGCTTTTAG", "aa": "MNEDKDENFKWYFSRDDLTIPSVEGLNFRHIRCYADNQQLKVIISGSRWGGWYSTYDKVESNVEDKILVKDGFDRF", "aa_hexdigest": "16378f58107b3c4b6ceee164ce8966fb", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF22346.2", "name": "CARDS_D3", "length": 68, "aa_cov": 0.8947368421052632, "hmm_cov": 1.0, "evalue": 4.727692292373223e-34, "score": 112.91462707519531, "start": 1, "stop": 68 } ], "seq_stats": { "molecular_weight": 9086.969899999996, "isoelectric_point": 4.984916114807128 }, "id": "APKECPOBEN_814", "locus": "GCA002090215_00790" }, { "type": "cds", "sequence": "contig_1", "start": 446180, "stop": 447037, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0035BC084C", "UniRef:UniRef90_UPI0035BC084C" ], "nt": "TTGAAGTCCAACGAGAGTTCTAAACGTGGTGTTTTAAGTCAAGCTGCTAGTGGTGCGGTGGTTGGTTATGTAGAGAGACTAACTAACAAGAAGGTTTTCTTAAAGGACGAAATTAAGTTTGATGTTGAAAAGGATGGCATCAAGCTAGACTTAAAAGCCGACTTCTCGCTTGAACAAAATGGCTCTTTAGGTTTGAATGACCCAAATGATGTCAACAAATTGGCACAGGAAGCACAATCTTACACAGTTATGGTAAGTGATGGCGGTGCCCAAACTGACAATAATGCTGAAGGTGGTAATCTAAGAATTGCCTTAACTAAGAATGCATTCAACCCAAACCAAAGCACTACGGTTGATATTCCTTACAAAATAGAAAATAGAAGTGTGGGAAATAATAAGGAGCAGAAAACACTTGTGTTTGATTTCTCTGGACTCAATCCGTATGAGTACAACATGATTGTAGGGGCATTGTTTACCGACAGCTCATTTATTAATGATGCTTATGCACCAATTCAAAGTACCTTCCAAAGACAATTGAAGGAGTTCTTACAAGTTAAGTACGAAAACCAAGTTGGTGCTAATGGTTCATTTGACCTCTTCAAACCACGCTCATTGTCTAGCCAACAACTAGTTCAAGGTGAAAGATCACTAGATGGCTTTACCGTTGAATTGAATGCAAATGGTGGCAGTTTCAACTTCTTAACCCACGTAGATCCGTTGGTAGCTGGTTTAACTGTGGCTGCTATAGCTAGTGTAGTAGTTGCTGGTGCTGTAACTTATTTAGTAGTACGTCGTTACCGCAAGCGCAACGAGTTTGTCGACAAGATCTTTGCTTCTAACATTAGAGCAAAACAGTGA", "aa": "MKSNESSKRGVLSQAASGAVVGYVERLTNKKVFLKDEIKFDVEKDGIKLDLKADFSLEQNGSLGLNDPNDVNKLAQEAQSYTVMVSDGGAQTDNNAEGGNLRIALTKNAFNPNQSTTVDIPYKIENRSVGNNKEQKTLVFDFSGLNPYEYNMIVGALFTDSSFINDAYAPIQSTFQRQLKEFLQVKYENQVGANGSFDLFKPRSLSSQQLVQGERSLDGFTVELNANGGSFNFLTHVDPLVAGLTVAAIASVVVAGAVTYLVVRRYRKRNEFVDKIFASNIRAKQ", "aa_hexdigest": "93356491d0a654c7e7a9da9a69a008e7", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0035BC084C", "query_cov": 1.0, "subject_cov": 0.9930313588850174, "identity": 1.0, "score": 542.0, "evalue": 2.07e-194, "valid": true, "uniref50_id": "UniRef50_UPI0035BC084C" }, "pscc": { "uniref50_id": "UniRef50_UPI0035BC084C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0035BC084C" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 31332.77299999999, "isoelectric_point": 6.643023109436036 }, "id": "APKECPOBEN_815", "locus": "GCA002090215_00791" }, { "type": "cds", "sequence": "contig_1", "start": 447116, "stop": 447409, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAACGGAAATGAAAGCAATGGTCACTTCTATGTGGTAGTAGATGTAACTAACGCTAACAACCTTGGTAACCAACGTCGTATTGCTAACCCTAGAAACTATTTCTATTACCTAGAAGTTTTAGACAAGAACGCGCAAAGTACTTACATTATCCGTTTTGAAACAAAGGATAAGTTCTACTCACTCGATGCACTTAGGTTCGACAACAACGGTATATACGTCGATAATGCCTCAAGGGATGCTGTAATTCAAGCAAGACAAAAATCTAAACTGTATTTCAACACTAGTGATTGA", "aa": "MNGNESNGHFYVVVDVTNANNLGNQRRIANPRNYFYYLEVLDKNAQSTYIIRFETKDKFYSLDALRFDNNGIYVDNASRDAVIQARQKSKLYFNTSD", "aa_hexdigest": "9ef9975b83d889d2665a65da8cb1bb44", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11315.330499999985, "isoelectric_point": 7.946776008605957 }, "id": "APKECPOBEN_816", "locus": "GCA002090215_00792" }, { "type": "cds", "sequence": "contig_1", "start": 447829, "stop": 447969, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCCATTTACGCTAACGTTACCCTAGGACAAAAAGTAACTAAGGAAGTTAAAGTGTGGGATGGACAAAACTTCCAGTTCTTAGCAATTGAAAACAATGAAGACCAAGCTCAATGCTTTAGCAAACGAAAACAAAGCTGA", "aa": "MAIYANVTLGQKVTKEVKVWDGQNFQFLAIENNEDQAQCFSKRKQS", "aa_hexdigest": "4652c0566a95aeb7e765497ed42c4a6b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5306.939300000001, "isoelectric_point": 7.884370613098144 }, "id": "APKECPOBEN_817", "locus": "GCA002090215_00793" }, { "type": "cds", "sequence": "contig_1", "start": 448072, "stop": 448506, "strand": "-", "frame": 1, "gene": null, "product": "putative protein MPN_374", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75407", "UniRef:UniRef90_P75407" ], "nt": "GTGAAAGAGGTGATTGACACTAACACCACTGGAACTAACTTTAAGTTTGAAAACAGAAATTACAAACTACAAAACCAAAGCTTTAGTTTTGTCCGTTACAAAAACTTTAACTTAGATAAGCTGTTGATCCCTAAAACAAACGCTAATCTAAATTCAACTAGAAACGGTGTTTTAACTCAAGCACCTACCTTAGATTTTGTTTTAAATCCAGTTTTTGTTAATGCAATCACTAACGTTTACAACCTTTCTGAACAAGTAAAGGATTTAGAACAACGTTTAGAGAGCAAAAGTAACGAAAGTGAAAAAGCTGGCATTAACAGCGTTTTAACGCAAATTAAAAACAAGCAAGTTGATTACCTAAAGGTAAAAGAATACATTAGTAGCCTAGATAAAGATACCAATAAAGTAAGTACTCCAATTAACTCAACCGGTTGA", "aa": "MKEVIDTNTTGTNFKFENRNYKLQNQSFSFVRYKNFNLDKLLIPKTNANLNSTRNGVLTQAPTLDFVLNPVFVNAITNVYNLSEQVKDLEQRLESKSNESEKAGINSVLTQIKNKQVDYLKVKEYISSLDKDTNKVSTPINSTG", "aa_hexdigest": "8cec4020a07a8bbd04c976782f08d0e6", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 447817, "stop": 448506, "observations": { "mutation": [ 447831, 448008, 448074 ], "stop": [ 447831, 448008, 448074 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75407", "query_cov": 1.0, "subject_cov": 0.62882096069869, "identity": 1.0, "score": 272.0, "evalue": 3.86e-91, "gene_start": 1, "gene_end": 144, "reference_start": 1, "reference_end": 144, "reference_sequence": "MKEVIDTNTTGTNFKFENRNYKLQNQSFSFVRYKNFNLDKLLIPKTNANLNSTRNGVLTQAPTLDFVLNPVFVNAITNVYNLSEQVKDLEQRLESKSNESEKAGINSVLTQIKNKQVDYLKVKEYISSLDKDTNKVSTPINSTGWVKWYQEANKELGLNLNQEPKDWDQFLKLVASYFSMAIYANVTLGQKVTKEVKVWDGQNFQFLAIENNEDQAQCFSKRKQSWNYC" }, "paralog": false, "identity": 0.982532751091703, "score": 446.0, "evalue": 7.96e-162, "description": "Internal stop codon at 447831, 448008, 448074. Nonsense mutation around 447831, 448008, 448074.", "psc": { "uniref90_id": "UniRef90_P75407", "product": "Uncharacterized protein MPN_374", "uniref50_id": "UniRef50_P75407", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75407", "UniRef:UniRef50_P75407" ] }, "pscc": { "uniref50_id": "UniRef50_P75407", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75407" ], "product": "Uncharacterized protein MPN_374" } }, "id": "APKECPOBEN_818", "locus": "GCA002090215_00794" }, { "type": "cds", "sequence": "contig_1", "start": 448852, "stop": 449043, "strand": "-", "frame": 1, "gene": null, "product": "putative protein MPN_375", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75406", "UniRef:UniRef90_P75406" ], "nt": "GTGCTCATTGAAAGGGGTGAACTTCACCGTCAAGCAGATTTAAAAAAATCTGACCCCGCAACAAACGAAACTTCCAAAACCGTTTGGGGTAGCATTAAAGAAAAGCTGTTACAAAACGTCAACAACCTCCACTCAGAGAAAGGAGTATTCTTATGGTTCCGTCAATCTGGTTTTACAACTACTAGAAACTAG", "aa": "MLIERGELHRQADLKKSDPATNETSKTVWGSIKEKLLQNVNNLHSEKGVFLWFRQSGFTTTRN", "aa_hexdigest": "fed5dd3bc384590074c0c74989a3d5c8", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 448852, "stop": 449241, "observations": { "mutation": [ 449085 ], "stop": [ 449085 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75406", "query_cov": 1.0, "subject_cov": 0.4883720930232558, "identity": 0.9840000000000001, "score": 129.0, "evalue": 2.32e-37, "gene_start": 1, "gene_end": 63, "reference_start": 67, "reference_end": 129, "reference_sequence": "MSFGLVGTVNNNGWKSPFRHETKYRAGYDKFKYYKTHYRGAKKAGTNDDRWRWTAWFDLDFAHQKIVLIERGELHRQADLKKSDPATNETSKTVWGSIKEKLLQNVNNLHSEKGVFLWFRQSGFTTTRN" }, "paralog": false, "identity": 0.9922480620155039, "score": 273.0, "evalue": 2.39e-96, "description": "Internal stop codon at 449085. Nonsense mutation around 449085.", "psc": { "uniref90_id": "UniRef90_P75406", "product": "Uncharacterized protein MPN_375", "uniref50_id": "UniRef50_P75406", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75406", "UniRef:UniRef50_P75406" ] }, "pscc": { "uniref50_id": "UniRef50_P75406", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75406" ], "product": "Uncharacterized protein MPN_375" } }, "id": "APKECPOBEN_819", "locus": "GCA002090215_00795" }, { "type": "cds", "sequence": "contig_1", "start": 449758, "stop": 451350, "strand": "-", "frame": 1, "gene": null, "product": "putative protein MPN_376", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P75405", "UniRef:UniRef90_P75405" ], "nt": "ATGAAGGTTTGGGATGGTGCTAAGTTTGAGTTCATCCCGATTGAAAACACTGAAAAACAATCAGAACAACTAGCAAATGAAAACCGCGCTGAGATTGCTGTAAGCGCCATTGGTTTTCAAGATGAAGGCGGTTTACGTGATGCTAGTTTTATCAATAAGGTAGCTCTAACCCCTAAGAGTAGTAAAACTAAAATTGCTAACGGTGATGCTAGCAAAATAGAAAAAGCAGCCAACGAAATTTCTTATAAGTACCATTACCGACAAAACTTTAAACAAGCTAGCTGGGACAAACAAAACTCACAAACCAAGAGTATTGTAGTACAAAGCACTGATTTGAACGATGAGCGCGAACGCTTCCAAAAGGACATTAACAACTATTTAAAGGTACAAGGCATCTCTGAAACTGAAATTAAAGTAAATGCTGTTCATAAAGTTGATGCGATGTTAAACGCCCGCAAAAGTGATGATCCGAAGCTCGCTAGCGTTCAAAGTACGGCAAACAAGTATGGTTTAAACCTACGATCTAACCCATACACTGGTCAGTTTTATGTAGTAGTGGATGTAACTAATGCCAATGACTTGGGTAACCAACGCCGGGCAAATAACGCTAAGAGTTACTTTTACTATATTGAAGGGTTGGACAAAGGAGCACAAAGTTCGTACTTAGTGCGCTTTGAAAACAAACAAAAGCTGTATTCACTCGAATCACTAGCGGTTGATAGTCGTGGCCTTTATGTGAAAAATGTGTCCAAGGATGCAATTATCCAAGCCAAGCAAAACCAAAACCTTTATCTCGATACTCATAACTGGAACGCTGCTTTGAAGGCTAACCTCACCAACGCTGAACTTACTTTGCCTACAGCAAGTGCTGACAATTCAGCTAAACTATCTACACCTAACGCTGAGAATGATGAGGGTTTTTTGAGTGAAAATGTCAGTGGTTCGATCTTAGGCTATGTTGAGCGAATGACTGGTAAAAAGCTCTTTTTAAAGGAAAGGGTGAGCTTTAATAAGGAAGACAAAAATAATTTAAAGCTACGTTTAACATCGAACTTTACCTTAGACAAGAAGGGTAATTTAGAGGTTAAAGACCCAAGTGTAATTAACCAAATTGTAGAAGAAGCTAAGGGTTACAATGTCTTGGTTTCAGAAGAAAAAGGTGATGATCCAGAATCAGACAAAAACATCTTCAAGATTACTTTAACCACCAACCCTGAACAAAGTACCGTTATTAAACTGCCTTACTGGATTGTTACCAAGAAATCCAAAACTAACAAGGATGGTACGGTTAGAGAGCAGAAAAACTTGGTGTTTGACTTCTCTAACCTAAACAACTTCGAATACAACACAGTTGTTAGTCTGTTGTTTACCGACAGCTCCTTTATTAAGAATGCCTACGCTCCATTGCAAACAGAATTTAGAAAGCAGCTCAAAACCGTTTTAGAACACAAATACCAAGCACCAATTAAGACTGGTCAACTACCTTTATTGACCAAGGTACAATTGGCAAATAACCAAAAACAAATTGATAACTTCACCTTTGATCTACACAAAAACATTTTCAATAAAGAAGACATCAATAAGATTAACTGA", "aa": "MKVWDGAKFEFIPIENTEKQSEQLANENRAEIAVSAIGFQDEGGLRDASFINKVALTPKSSKTKIANGDASKIEKAANEISYKYHYRQNFKQASWDKQNSQTKSIVVQSTDLNDERERFQKDINNYLKVQGISETEIKVNAVHKVDAMLNARKSDDPKLASVQSTANKYGLNLRSNPYTGQFYVVVDVTNANDLGNQRRANNAKSYFYYIEGLDKGAQSSYLVRFENKQKLYSLESLAVDSRGLYVKNVSKDAIIQAKQNQNLYLDTHNWNAALKANLTNAELTLPTASADNSAKLSTPNAENDEGFLSENVSGSILGYVERMTGKKLFLKERVSFNKEDKNNLKLRLTSNFTLDKKGNLEVKDPSVINQIVEEAKGYNVLVSEEKGDDPESDKNIFKITLTTNPEQSTVIKLPYWIVTKKSKTNKDGTVREQKNLVFDFSNLNNFEYNTVVSLLFTDSSFIKNAYAPLQTEFRKQLKTVLEHKYQAPIKTGQLPLLTKVQLANNQKQIDNFTFDLHKNIFNKEDINKIN", "aa_hexdigest": "870cdcc48cbd6877478a28c061d72697", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 449620, "stop": 451650, "observations": { "mutation": [ 449655, 449679, 449760, 451437, 451545 ], "stop": [ 449655, 449679, 449760, 451437, 451545 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75405", "query_cov": 1.0, "subject_cov": 0.4649122807017544, "identity": 1.0, "score": 1013.0, "evalue": 0.0, "gene_start": 1, "gene_end": 530, "reference_start": 565, "reference_end": 1094, "reference_sequence": "MKRLQYRFLLFGFALGSFGWFVASSAFTSVVKSNNETLSSDGGVHMRYLKGKGDVLSILKSKSPAITQRFDLKWNNGKKTEVYLNNVNNYISESLYAQEALKQIERTAQLSIQNGELPDVKVYTNSGASFGLVGWHTDNGRTWAFRHENKYDKNVHFSWNQTYYKYSNRERTASNPYYWKWVAWFDLGYANQRIGLVENDEYHIDKRVPEPTPRKWDKNKPLWGDIRSKILYSAERLDPDKGIFIWFNQTGFNTKGTKGWANSGFFTDFWDTNNNPNAFTTNITSEGGNSNWHSPDWGSHTTDTRFFLKLEPYSKLFYKENGQERSITVSDYIRKAKSTKTNYQWVNKNQIKTLVRKTRSIDLGLGSAVRQTYTTKSDIASNQQLKYKLKDSTFSIDTYNNFKLDKLLVPKTNEDATAIKNGVFVKQPTLSFDFNPVLTNAIVNIHNLFAQTLDLKEHLKSDQPYNESDKAAINKVIEQIKNKEVDYIQVADFIGKLKNWSQNPGSIESKGENTAQWYADAKREFGLNLNDDVNTWTQLSSLIASYFSKDIFANVKLNGAKERRMKVWDGAKFEFIPIENTEKQSEQLANENRAEIAVSAIGFQDEGGLRDASFINKVALTPKSSKTKIANGDASKIEKAANEISYKYHYRQNFKQASWDKQNSQTKSIVVQSTDLNDERERFQKDINNYLKVQGISETEIKVNAVHKVDAMLNARKSDDPKLASVQSTANKYGLNLRSNPYTGQFYVVVDVTNANDLGNQRRANNAKSYFYYIEGLDKGAQSSYLVRFENKQKLYSLESLAVDSRGLYVKNVSKDAIIQAKQNQNLYLDTHNWNAALKANLTNAELTLPTASADNSAKLSTPNAENDEGFLSENVSGSILGYVERMTGKKLFLKERVSFNKEDKNNLKLRLTSNFTLDKKGNLEVKDPSVINQIVEEAKGYNVLVSEEKGDDPESDKNIFKITLTTNPEQSTVIKLPYWIVTKKSKTNKDGTVREQKNLVFDFSNLNNFEYNTVVSLLFTDSSFIKNAYAPLQTEFRKQLKTVLEHKYQAPIKTGQLPLLTKVQLANNQKQIDNFTFDLHKNIFNKEDINKINWPLIAITFTGSAALLSTIIASGVVLHRWRKSRKHFWEQMLKARKVK" }, "paralog": false, "identity": 0.992603550295858, "score": 1307.0, "evalue": 0.0, "description": "Internal stop codon at 449655, 449679, 449760, 451437, 451545. Nonsense mutation around 449655, 449679, 449760, 451437, 451545.", "psc": { "uniref90_id": "UniRef90_P75405", "product": "Uncharacterized protein MPN_376", "uniref50_id": "UniRef50_P75405", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75405", "UniRef:UniRef50_P75405", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75405", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75405" ], "product": "Uncharacterized protein MPN_376" } }, "id": "APKECPOBEN_820", "locus": "GCA002090215_00796" }, { "type": "cds", "sequence": "contig_1", "start": 451543, "stop": 451818, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCCAAAAACCAACGAGGATGCTACGGCAATTAAGAATGGTGTTTTTGTCAAACAACCAACACTGTCCTTTGACTTTAACCCCGTTTTGACAAACGCCATTGTCAACATTCACAATCTGTTTGCGCAAACACTTGATTTAAAGGAGCATTTAAAGTCTGATCAACCTTACAATGAAAGTGACAAAGCAGCAATCAATAAGGTTATAGAACAAATCAAAAATAAGGAAGTTGACTACATTCAAGTAGCTGATTTTATTGGCAAGCTGAAAAATTGA", "aa": "MPKTNEDATAIKNGVFVKQPTLSFDFNPVLTNAIVNIHNLFAQTLDLKEHLKSDQPYNESDKAAINKVIEQIKNKEVDYIQVADFIGKLKN", "aa_hexdigest": "c56e106ad43a8b3a1c109fc22c6d28d9", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10286.595499999992, "isoelectric_point": 6.056785774230956 }, "id": "APKECPOBEN_821", "locus": "GCA002090215_00797" }, { "type": "cds", "sequence": "contig_1", "start": 452152, "stop": 452499, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGCTTGGTTTGATTTGGGTTATGCCAACCAACGCATAGGTTTGGTGGAAAATGATGAATATCATATTGACAAGCGTGTTCCAGAACCAACTCCCAGAAAATGGGATAAAAACAAACCTTTATGGGGTGATATTCGCTCGAAGATTCTCTACAGTGCTGAGCGGTTAGATCCAGATAAGGGAATCTTCATCTGGTTCAACCAAACTGGTTTTAACACCAAGGGTACTAAGGGCTGGGCTAACAGTGGCTTTTTTACCGATTTTTGGGATACCAACAATAACCCCAATGCCTTTACCACCAACATCACTTCTGAAGGTGGTAACTCCAATTGGCACAGTCCTGATTGA", "aa": "MAWFDLGYANQRIGLVENDEYHIDKRVPEPTPRKWDKNKPLWGDIRSKILYSAERLDPDKGIFIWFNQTGFNTKGTKGWANSGFFTDFWDTNNNPNAFTTNITSEGGNSNWHSPD", "aa_hexdigest": "1557b8f18486d770c223a0cfc85380d7", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13330.473299999983, "isoelectric_point": 5.623558998107909 }, "id": "APKECPOBEN_822", "locus": "GCA002090215_00798" }, { "type": "tRNA", "sequence": "contig_1", "start": 453307, "stop": 453383, "strand": "-", "gene": "trnR", "product": "tRNA-Arg(tcg)", "amino_acid": "Arg", "anti_codon": "tcg", "score": 74.9, "nt": "GCGCCCATAGCTCAATTGGATAGAGTGTCTGGCTTCGGACCAGAAGGTTATGGGTTCAAGTCCTATTGGGCGCGCCA", "db_xrefs": [ "SO:0001036" ], "anti_codon_pos": [ 453347, 453349 ], "id": "APKECPOBEN_32", "locus": "GCA002090215_00799" }, { "type": "cds", "sequence": "contig_1", "start": 453469, "stop": 453693, "strand": "+", "frame": 1, "gene": null, "product": "putative protein MPN_377", "db_xrefs": [ "RefSeq:WP_010874733.1", "SO:0001217", "UniParc:UPI000013A8AE", "UniRef:UniRef100_Q9EXD3", "UniRef:UniRef50_Q9EXD3", "UniRef:UniRef90_Q9EXD3" ], "nt": "ATGTCAAAGGATAAAAAAAATAAGGTTGAACAGCTTGAACCAGTAGACCTTTTTGAGCGGACTAAGCTAGAGGACACCCAAGTTTTAAATGATGTTGAACTAGATGACATCAAGAAACTAGAAGAGTTGAAAAAAGAATTGGAAAACACCTTTGAGCCACGTACCAGAATTGAAATTAAACGTGAAATCAAAGAGTTGGAACGTAAACTAAGAAGGAACCGTTAG", "aa": "MSKDKKNKVEQLEPVDLFERTKLEDTQVLNDVELDDIKKLEELKKELENTFEPRTRIEIKREIKELERKLRRNR", "aa_hexdigest": "6980de67e95994fd57e9f42468833157", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013A8AE", "ncbi_nrp_id": "WP_010874733.1", "uniref100_id": "UniRef100_Q9EXD3", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013A8AE", "RefSeq:WP_010874733.1", "UniRef:UniRef100_Q9EXD3" ] }, "ips": { "uniref100_id": "UniRef100_Q9EXD3", "uniref90_id": "UniRef90_Q9EXD3" }, "psc": { "uniref90_id": "UniRef90_Q9EXD3", "product": "Uncharacterized protein MPN_377", "uniref50_id": "UniRef50_Q9EXD3" }, "pscc": { "uniref50_id": "UniRef50_Q9EXD3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9EXD3" ], "product": "Uncharacterized protein MPN_377" }, "genes": [], "id": "APKECPOBEN_823", "locus": "GCA002090215_00800" }, { "type": "cds", "sequence": "contig_1", "start": 453697, "stop": 454233, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTGTTAACCTCCACACTAACTCTTACTATAACTTCCTCAACTCCACACTTTCACCGCAAAAGCTAGTTGACCTAGCTGTTCAAGACCAACAGGTAGCTGTTTGTTTAACCGATCCCAACTTATTTGGTGCTACCGAGTTTTTTTTGGCCTGTCAAAAAGCGCACATTAAACCCTTAATTGGGTTGAGTGTTACTGTTCGACATTACGAACAAAACGTTAATTTACTAGTAATTGCCCAAACCAACCGGGGTTATCAGAACCTAATGTGCTTAGCGTTAGTTAAAGACCAACCAGATTTGCAATTAGAACCCTTTTTGGATGGTAATGTGGTAATTATTTGCACACAAACTGAACTGCGCCTCAACACCGCAAACCCCGTTTACTTAGCACATGGTTTGAGTGGTCGTTATCCTAAAATTGCAGTAACACAAAAACCAGTTAAGTGTCAAAACACCAATAAGGACTTAACACTGCTCTTAACACTTAAGCAAATTAGTCAAATTAACAGCGAACACTTCCAGCCACTGGAATGA", "aa": "MFVNLHTNSYYNFLNSTLSPQKLVDLAVQDQQVAVCLTDPNLFGATEFFLACQKAHIKPLIGLSVTVRHYEQNVNLLVIAQTNRGYQNLMCLALVKDQPDLQLEPFLDGNVVIICTQTELRLNTANPVYLAHGLSGRYPKIAVTQKPVKCQNTNKDLTLLLTLKQISQINSEHFQPLE", "aa_hexdigest": "3209869950fa494f0036ef272393cc74", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 20022.923600000006, "isoelectric_point": 6.943928718566894 }, "id": "APKECPOBEN_824", "locus": "GCA002090215_00801" }, { "type": "cds", "sequence": "contig_1", "start": 454420, "stop": 455820, "strand": "+", "frame": 1, "gene": "dnaE", "product": "DNA polymerase III subunit alpha", "db_xrefs": [ "COG:COG0587", "COG:L", "EC:2.7.7.7", "GO:0003887", "GO:0005737", "GO:0006260", "GO:0008408", "SO:0001217", "UniRef:UniRef50_Q49405", "UniRef:UniRef90_P75404" ], "nt": "GTGTCACACAACGATGCACAAATGTTAAGCGAGCAGTGTCAACTAGCCTTAACTGAATTTTTAAAGCTCAATCCGCAATTAAACAAACAGCTCTATGAAGAGCGGTTAGCCAAAGAATTGGAGATAATTAACTCACTCCACTTTGCTAGTTACTTTTTAGTGGTGAGCGACTTAGTTCAGTTTGCCCATAATAACGATATCCTCATCGGTCCTGGTCGAGGATCTGCAGTGGGTTCTTTAGTGGCCTTTCTGCTCAAAATTACCCAAATTGATCCAGTAGCCAACAATCTCATTTTTGAGCGCTTTATTTCCCGTCACCGCCAGGGCTTACCTGACATTGACATTGACATTATGGAAACTAAACGTGATTTAGTAATTGACTATGTCATGCAAAAATATGGCCGGGAACAGTGTGCACAAATAGTTACTTTCCAAAAATTTAAGACCCGCTCTGCGTTGCGTGATGTCGGTAAGGTATTTAACCACATTGAGGGTGCTGAAGATTTACTGGGTAAACTACCAAAAGACAAAAGCTTATTGGAGCTTGATGTAAACGGTGTTACTGATCCGGTTTTACAACTTAGTTTAAAGAGCTTTCGCTTGTTGTGGGAGGTGGCGCGTGAAATTATTAACTTTCCGCGCCAACCAAGCATTCACGCTTCTGGTGTTGTGATTGTCACTGAACCTTTAATTACCACGATCCCTTTAATGGTGGGTAATAACAACAACTACGTCACCCAAGTGTCGATGGACTGGTTAGAATGGTACAACCTCAATAAGTTTGACTTATTGGGTTTAATTAACCTCACCATGATCCATGAGGTTGTGCAAGCGGTAAAACCCAAGGAAGTTAGCGTACAGCAATTCCTCCAACAAATACCGCTAGATGATGAAGCTACTTTTACTAACCTCACTAACCAAGCCACTTTAGGTGTTTTTCAACTAGAGTCGTTTGGGATGAAAAAGGTGCTCAAACAAATTAAACCGCACAACCTCCATGATTTAGCAATTGTTTTAGCACTTTACCGCCCCGGTCCACAGGACAACATTAATACCTTTATTGCTAACCGTAACTTAGGGTTTGACACATCAGACATCGATCCGCGAATTTTGCCAATCCTCAAAGAAACTTACGGAGTGTTAATCTTTCAAGAACAGGTTATTAACATCGCCAAAACTGTTGCTAACTATTCATTGGAAACCGCTGATAGCTTTCGCCGTGCCATTTCCAAAAAAAACTTGCAAGTAATTCAGGACAACATGCGCAGCTTTTATGAAGGCGCGCTAGCCAATAACTTTAGCTTAAAAGCAGCGACTACTATCTTTAATTACATCCAGCGCTTTGCTGGATATGGTTTTAACTTGTCCCACGCCTTGGGCTATGCTTTATTGAGTTACTGA", "aa": "MSHNDAQMLSEQCQLALTEFLKLNPQLNKQLYEERLAKELEIINSLHFASYFLVVSDLVQFAHNNDILIGPGRGSAVGSLVAFLLKITQIDPVANNLIFERFISRHRQGLPDIDIDIMETKRDLVIDYVMQKYGREQCAQIVTFQKFKTRSALRDVGKVFNHIEGAEDLLGKLPKDKSLLELDVNGVTDPVLQLSLKSFRLLWEVAREIINFPRQPSIHASGVVIVTEPLITTIPLMVGNNNNYVTQVSMDWLEWYNLNKFDLLGLINLTMIHEVVQAVKPKEVSVQQFLQQIPLDDEATFTNLTNQATLGVFQLESFGMKKVLKQIKPHNLHDLAIVLALYRPGPQDNINTFIANRNLGFDTSDIDPRILPILKETYGVLIFQEQVINIAKTVANYSLETADSFRRAISKKNLQVIQDNMRSFYEGALANNFSLKAATTIFNYIQRFAGYGFNLSHALGYALLSY", "aa_hexdigest": "089fccb277eed0c9f9467c5cd19169af", "start_type": "GTG", "rbs_motif": null, "genes": [ "dnaE" ], "truncated": "both", "pseudogene": { "start": 454120, "stop": 456123, "observations": { "mutation": [ 454231, 454384, 455818, 455827, 455866 ], "stop": [ 454231, 454384, 455818, 455827, 455866 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75404", "query_cov": 1.0, "subject_cov": 0.5344036697247706, "identity": 0.998, "score": 910.0, "evalue": 0.0, "gene_start": 1, "gene_end": 466, "reference_start": 242, "reference_end": 707, "reference_sequence": "MFVNLHTNSYYNFLNSTLSPQKLVDLAVQDQQVAVCLTDPNLFGATEFFLACQKAHIKPLIGLSVTVRHYEQNVNLLVIAQTNRGYQNLMCLALVKDQPDLQLEPFLDGNVVIICTQTELRLNTANPVYLAHGLSGRYPKIAVTQKPVKCQNTNKDLTLLLTLKQISQINSEHFQPLEWKLSRSLNEIQLDPPLLQQLRHQPFLSQKAAQQIFSEEELGNLQKLVEQSQWDLTKLKASSLQVSHNDAQMLSEQCQLALTEFLKLNPQLNKQLYEERLAKELEIINSLHFASYFLVVSDLVQFAHNNDILIGPGRGSAVGSLVAFLLKITQIDPVANNLIFERFISRHRQGLPDIDIDIMETKRDLVIDYVMQKYGREQCAQIVTFQKFKTRSALRDVGKVFNHIEGAEDLLGKLPKDKSLLELDVNGVTDPVLQLSLKSFRLLWEVAREIINFPRQPSIHASGVVIVTEPLITTIPLMVGNNNNYVTQVSMDWLEWYNLNKFDLLGLINLTMIHEVVQAVKPKEVSVQQFLQQIPLDDEATFTNLTNQATLGVFQLESFGMKKVLKQIKPHNLHDLAIVLALYRPGPQDNINTFIANRNLGFDTSDIDPRILPILKETYGVLIFQEQVINIAKTVANYSLETADSFRRAISKKNLQVIQDNMRSFYEGALANNFSLKAATTIFNYIQRFAGYGFNLSHALGYALLSYWTAWLKTHYFEQFNLWWLNHEQGKKEKQKQLLNEFISSGYEICPPLINKAKSDFSVQDKKLYLGFKLINGIGDKQAHALEHVQEVLKQNPNLSLIATVNLCLSKTVGGLELKDITLLQQAGCFNCFNYTVDFNLAKSFWVQSNHELFPKIPLDQPPVINWKSFGF" }, "paralog": false, "identity": 0.992503748125937, "score": 1293.0, "evalue": 0.0, "description": "Internal stop codon at 454231, 454384, 455818, 455827, 455866. Nonsense mutation around 454231, 454384, 455818, 455827, 455866.", "psc": { "uniref90_id": "UniRef90_P75404", "gene": "dnaE", "product": "DNA polymerase III subunit alpha", "ec_ids": [ "2.7.7.7" ], "uniref50_id": "UniRef50_Q49405", "cog_id": "COG0587", "cog_category": "L", "go_ids": [ "GO:0005737", "GO:0008408", "GO:0003887", "GO:0006260" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75404", "EC:2.7.7.7", "UniRef:UniRef50_Q49405", "COG:COG0587", "COG:L", "GO:0005737", "GO:0008408", "GO:0003887", "GO:0006260" ] }, "pscc": { "uniref50_id": "UniRef50_Q49405", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49405" ], "product": "DNA polymerase III subunit alpha" } }, "id": "APKECPOBEN_825", "locus": "GCA002090215_00802" }, { "type": "cds", "sequence": "contig_1", "start": 456052, "stop": 456297, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAACATGTTCAGGAAGTTTTAAAGCAAAACCCCAATTTATCCTTAATAGCTACCGTTAATTTGTGTTTGAGCAAAACGGTAGGGGGTTTAGAACTTAAAGACATTACATTGCTCCAACAAGCAGGTTGTTTTAATTGCTTTAATTACACGGTTGACTTTAATTTGGCCAAAAGCTTTTGGGTGCAAAGTAACCATGAATTATTTCCAAAAATACCACTAGATCAACCACCCGTAATTAACTGA", "aa": "MEHVQEVLKQNPNLSLIATVNLCLSKTVGGLELKDITLLQQAGCFNCFNYTVDFNLAKSFWVQSNHELFPKIPLDQPPVIN", "aa_hexdigest": "014008d0d84c6781f1abcd1553ee6881", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9129.473399999993, "isoelectric_point": 5.453553581237792 }, "id": "APKECPOBEN_826", "locus": "GCA002090215_00803" }, { "type": "cds", "sequence": "contig_1", "start": 456631, "stop": 457194, "strand": "+", "frame": 1, "gene": "polA", "product": "5'-3' exonuclease", "db_xrefs": [ "COG:COG0258", "COG:L", "EC:3.1.11.-", "GO:0003677", "GO:0008409", "GO:0017108", "GO:0033567", "SO:0001217", "UniRef:UniRef50_P75403", "UniRef:UniRef90_P75403" ], "nt": "GTGTGTGAACAAGACGGGTTTGAGGCAGATGATCTAATTGGTAGTTATGCGAGGTTAATGACACAAAACAATGTGGCAGTAGACATCTACAGCTCTGACCGTGATTTACTACAGTTAGTCGATAGTATGACTAGTGTTTGGCTTTGTGTAAAGGGCACAAAAGAGATGAAGGAATATAACACTGACAACTTTGCTGAGCAGTTTTTTGGCTTAACACCAAAACAAGTGATTGAATATAAAGGTTTGGTAGGGGATAACAGTGATAACCTCACTGGCATTAAGGGAATTGGTCCGAAAAAAGGGATTGATCTACTCAAACAATACGGTACCATTGACAATATTTTTGCTAACTTTGACAAGCTATCCAAAGCATTGCAAACGATTCTCCAAGGCCAAATTGACACTGCTAAGAAATTCAGCTTTTTAGCGAGCATTAAAACAGATATTAAACTTAATGACGATATTGTCCACGCTGCTTTAAAACCAATCGATAAACAAGCTTTATTAGAGCTTTTAGATAAATATGGCATTAAAGCACTAGCGCAAAAATTTAGTCAACTGTAA", "aa": "MCEQDGFEADDLIGSYARLMTQNNVAVDIYSSDRDLLQLVDSMTSVWLCVKGTKEMKEYNTDNFAEQFFGLTPKQVIEYKGLVGDNSDNLTGIKGIGPKKGIDLLKQYGTIDNIFANFDKLSKALQTILQGQIDTAKKFSFLASIKTDIKLNDDIVHAALKPIDKQALLELLDKYGIKALAQKFSQL", "aa_hexdigest": "daef5f8d5d43e87d2333fe85ad5f3a91", "start_type": "GTG", "rbs_motif": null, "genes": [ "polA" ], "truncated": "5-prime", "pseudogene": { "start": 456331, "stop": 457194, "observations": { "mutation": [ 456379, 456454 ], "stop": [ 456379, 456454 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75403", "query_cov": 1.0, "subject_cov": 0.6426116838487973, "identity": 0.995, "score": 369.0, "evalue": 8.07e-128, "gene_start": 1, "gene_end": 187, "reference_start": 105, "reference_end": 291, "reference_sequence": "MKNAILIDGNSLAYRAYFATWQQVEFAKLHNLPFNNAIRTMLMMCWNLLQSKQYDYGVISFDTKAPTFRDQLYSEYKSKRSKTPSELLVQIPVVKESLRHLGFLVCEQDGFEADDLIGSYARLMTQNNVAVDIYSSDRDLLQLVDSMTSVWLCVKGTKEMKEYNTDNFAEQFFGLTPKQVIEYKGLVGDNSDNLTGIKGIGPKKGIDLLKQYGTIDNIFANFDKLSKALQTILQGQIDTAKKFSFLASIKTDIKLNDDIVHAALKPIDKQALLELLDKYGIKALAQKFSQL" }, "paralog": false, "identity": 0.9930313588850174, "score": 560.0, "evalue": 2.43e-205, "description": "Internal stop codon at 456379, 456454. Nonsense mutation around 456379, 456454.", "psc": { "uniref90_id": "UniRef90_P75403", "gene": "polA", "product": "5'-3' exonuclease", "ec_ids": [ "3.1.11.-" ], "uniref50_id": "UniRef50_P75403", "cog_id": "COG0258", "cog_category": "L", "go_ids": [ "GO:0008409", "GO:0017108", "GO:0003677", "GO:0033567" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75403", "EC:3.1.11.-", "UniRef:UniRef50_P75403", "COG:COG0258", "COG:L", "GO:0008409", "GO:0017108", "GO:0003677", "GO:0033567" ] }, "pscc": { "uniref50_id": "UniRef50_P75403", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75403" ], "product": "5'-3' exonuclease" } }, "id": "APKECPOBEN_827", "locus": "GCA002090215_00804" }, { "type": "cds", "sequence": "contig_1", "start": 457194, "stop": 458027, "strand": "+", "frame": 3, "gene": "mutM", "product": "DNA-formamidopyrimidine glycosylase", "db_xrefs": [ "COG:COG0266", "COG:L", "EC:3.2.2.23", "GO:0003684", "GO:0005737", "GO:0006281", "GO:0006284", "GO:0008270", "GO:0008534", "GO:0034039", "GO:0046872", "GO:0140078", "KEGG:K10563", "RefSeq:WP_010874736.1", "SO:0001217", "UniParc:UPI000012AB8D", "UniRef:UniRef100_P75402", "UniRef:UniRef50_P55825", "UniRef:UniRef90_P75402" ], "nt": "ATGCCTGAACTACCTGAAGTTGCCACTGTAATTACCGAACTTAAGAGCTGTGTTTTAAACAAACCTGTCAAGCAAGTTAAGGTTCACTTAGACAAGGTGTTGAAAAATACTAATGTCAAGCAGCTTAACGATGCGTTAGTCAACCACAGCTTTGTGGACATTAAGCGCCGCGGCAAGTACATTATTTTTTGTTTAAGTAACGGTTTGTTTTTAGTGTCACACTTACGCATGGAAGGCAAATATTTCTTTGAAGCTAAAGGCAGTCAGTTTGACTTAAACCATGTTTTAGTCGAGTTTTTATTCCAAGATGGTGATCAACTTAACTATCATGATACGCGTCAATTTGGTACCTTTCACTTGTTCAATAGATATCAGTTTGAAAACGCACGTGAATTGAACAAATTAGCACTCGATCCCTTAGATCAGGAATTTAACCATCAAGCGATCTTTAACAAAGGCCATAAGTCCAACAAGAAGATTAAAACCTTTATTTTAGATCAGACCAACATTAGCGGTATTGGCAATATTTACGCTGATGAAATTTTGTTTGCAAGTAAGATTCACCCTGAAACTTTAGCTAAAAATTTGAACTTATCGCAGTATCAGCTAATTTGTCAAAATGCAACTGATATTTTAAAGAAAGCAGTTGAAATGAAGGGTACTACCATTGGCACTTTTACCTTTAAAAAAGATCATACCGGTGGTTACCAACACTTCTTGAAAATTCATGGCAAAAAGGGGAAGCAATGTCAAAGCTGTAACACCACTATTATTAAGAAAAAGATTAATGGTCGGGGCAGCTATATTTGTGAAAAGTGTCAAATACAGCGTTAA", "aa": "MPELPEVATVITELKSCVLNKPVKQVKVHLDKVLKNTNVKQLNDALVNHSFVDIKRRGKYIIFCLSNGLFLVSHLRMEGKYFFEAKGSQFDLNHVLVEFLFQDGDQLNYHDTRQFGTFHLFNRYQFENARELNKLALDPLDQEFNHQAIFNKGHKSNKKIKTFILDQTNISGIGNIYADEILFASKIHPETLAKNLNLSQYQLICQNATDILKKAVEMKGTTIGTFTFKKDHTGGYQHFLKIHGKKGKQCQSCNTTIIKKKINGRGSYICEKCQIQR", "aa_hexdigest": "d964a67a07a0bb109692ac793c81463c", "start_type": "ATG", "rbs_motif": "AAAA", "ups": { "uniparc_id": "UPI000012AB8D", "ncbi_nrp_id": "WP_010874736.1", "uniref100_id": "UniRef100_P75402", "db_xrefs": [ "SO:0001217", "UniParc:UPI000012AB8D", "RefSeq:WP_010874736.1", "UniRef:UniRef100_P75402" ] }, "ips": { "uniref100_id": "UniRef100_P75402", "uniref90_id": "UniRef90_P75402" }, "psc": { "uniref90_id": "UniRef90_P75402", "gene": "mutM", "product": "DNA-formamidopyrimidine glycosylase", "ec_ids": [ "3.2.2.23" ], "uniref50_id": "UniRef50_P55825", "cog_id": "COG0266", "cog_category": "L", "kegg_orthology_id": "K10563", "go_ids": [ "GO:0003684", "GO:0005737", "GO:0006281", "GO:0006284", "GO:0008270", "GO:0008534", "GO:0034039", "GO:0046872", "GO:0140078" ] }, "pscc": { "uniref50_id": "UniRef50_P55825", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P55825" ], "product": "Formamidopyrimidine-DNA glycosylase" }, "genes": [ "mutM" ], "id": "APKECPOBEN_828", "locus": "GCA002090215_00805" }, { "type": "cds", "sequence": "contig_1", "start": 458347, "stop": 458550, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08282.18" ], "nt": "GTGACTGGCCGTGATTACTGTCGCGTTCGTTGGTTAGAAAAGTACCTAAACACTGATCTACCAACAATTACCCTCGATGGCGCCATTATTTACTTCCGCGATGAAATTATTCGGCAAGAAGTGCTCGATAAGGAATTTATCCATGGCATTAACCAGATTGTCAAACGTTACCCTACAGCAGCCTTTAAGCTCAACATGGGTTGA", "aa": "MTGRDYCRVRWLEKYLNTDLPTITLDGAIIYFRDEIIRQEVLDKEFIHGINQIVKRYPTAAFKLNMG", "aa_hexdigest": "10eb145111f806d704660ca97303c7db", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08282.18", "name": "Hydrolase_3", "length": 58, "aa_cov": 0.8507462686567164, "hmm_cov": 1.0, "evalue": 1.814617245240887e-07, "score": 26.570499420166016, "start": 2, "stop": 58 } ], "seq_stats": { "molecular_weight": 7964.164399999998, "isoelectric_point": 7.879986763000487 }, "id": "APKECPOBEN_829", "locus": "GCA002090215_00806" }, { "type": "cds", "sequence": "contig_1", "start": 458680, "stop": 458928, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08282.18" ], "nt": "ATGAAAGTAAATCAGGTTTACTTTGACACATTTACTTGCCCTGAACCAATGATCCAAGAGCTCGATAACTTAGTTGAAAAAAGTGATGTAACCGCCAAAAGCTACCGCCATTCACTTTACATTATTAAAAAAGGGGTATCGAAGGCTAGTGCACTACAAAACCTCCAACGAGACTTTTTAGTGGAAATGAAGCCTGCTAACACGATTGTCTTTGGTGATGGGGATAATGACATTGAAATGATGCAGTGA", "aa": "MKVNQVYFDTFTCPEPMIQELDNLVEKSDVTAKSYRHSLYIIKKGVSKASALQNLQRDFLVEMKPANTIVFGDGDNDIEMMQ", "aa_hexdigest": "39b9727459c323d7ef78bc4b7d0bf32e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08282.18", "name": "Hydrolase_3", "length": 68, "aa_cov": 0.8292682926829268, "hmm_cov": 0.9705882352941176, "evalue": 3.940289046114921e-14, "score": 48.40382766723633, "start": 15, "stop": 82 } ], "seq_stats": { "molecular_weight": 9389.674399999998, "isoelectric_point": 4.93211269378662 }, "id": "APKECPOBEN_830", "locus": "GCA002090215_00807" }, { "type": "cds", "sequence": "contig_1", "start": 458941, "stop": 459051, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTCGTTAACTGGTAGTGATCCCGAGTGTTATAAGTTAGCTAAGTACCACACCGACAGCGTTGACGATGATGGGATTGCCAAATGGATTAATAAAAATCTGTTATGTTAA", "aa": "MSLTGSDPECYKLAKYHTDSVDDDGIAKWINKNLLC", "aa_hexdigest": "5b405e6f40582844bc0e46a4ed34cd66", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4045.5279999999993, "isoelectric_point": 4.822015571594237 }, "id": "APKECPOBEN_831", "locus": "GCA002090215_00808" }, { "type": "cds", "sequence": "contig_1", "start": 459045, "stop": 459323, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01121.26" ], "nt": "ATGTTAATCGCTGTTGTAGGCAAAGCTGGGGTTGGTAAAACCACCGTTTTGCAATACATCGCTGACTATTTTCATTTTCCCGTTTTTTTTGCTGATCGGTTTATCCATCAACAATACGCAAATGGTCAAGCTGGTTATGCAATTGTAAAACAGCAATTTGGTGCTCAGTTTGTCAATCATGAAGCAGTTGATCGCAAACAGTTAGCACAATACGTTTTTAATCAACCTGATGAGTTAAAGCGTTTGTCCAACTTAACCAAACCCCTAGTACAAGAGTGA", "aa": "MLIAVVGKAGVGKTTVLQYIADYFHFPVFFADRFIHQQYANGQAGYAIVKQQFGAQFVNHEAVDRKQLAQYVFNQPDELKRLSNLTKPLVQE", "aa_hexdigest": "7443c091d42745058c1f67b8fe99bff9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01121.26", "name": "CoaE", "length": 92, "aa_cov": 0.9891304347826086, "hmm_cov": 0.9891304347826086, "evalue": 3.219155417117507e-14, "score": 48.453372955322266, "start": 1, "stop": 91 } ], "seq_stats": { "molecular_weight": 10475.864699999995, "isoelectric_point": 9.004637718200684 }, "id": "APKECPOBEN_832", "locus": "GCA002090215_00809" }, { "type": "cds", "sequence": "contig_1", "start": 459577, "stop": 460425, "strand": "-", "frame": 1, "gene": "cof", "product": "Putative phosphatase MPN_383", "db_xrefs": [ "COG:COG0561", "COG:HR", "EC:3.1.3.-", "GO:0000287", "GO:0005829", "GO:0016791", "KEGG:K21064", "RefSeq:WP_010874739.1", "SO:0001217", "UniParc:UPI0000139536", "UniRef:UniRef100_P75399", "UniRef:UniRef50_P75399", "UniRef:UniRef90_P75399" ], "nt": "ATGAAACCAAAAGTACAAAACTTAATTTTTGACCTCGATGGTACTTTATTGTCTTGGGGTCATGAACCACTACCACAAACTGTTACTTTTCTCAAAGAGTTGCAAAAGCAGGGGTTTAAAATTACCTTTGCGACTGGGCGCAGTCATATCTTAATCCGCAATACCACTCAGTTTATTCAACCTGATCTACCGGTTATTTCCTCTAACGGTGCTTTAATTTATGACTTTGCTCGGGAAAAAGCGCTGCATATGACCCAGTTAGCACCCCAAAGTGTGGTACCCATTATGCGTTTACTGTTGCAATTGGAAGAAAGCTTTTGCATTTACACTGATAAAAAGGTGTTTGGTTTTGAAAAACCCGGTATACCTTGCAAACGGTTACGCACTACTCAAAGCAAAATTGTAGAACCTGACATTACACAAAATAACTTTACCATTAATCCGTTAACTGATGCTAGCAAGTTTGATTTTGCCACACAAAACATTACCAAGATTCTTTTAATCACTGAAGACAGGGGTCGCATTAGTAAAATAACCAAACATCTCGATGCGATTGAAAACATTAGCTATGTCAGTTCTATGACCTTTGCGCTTGACATTATGCACAAAGATGTCAATAAGGCATACGGTCTCAAAGCATTAGAACAGCAAACCGGTTTAGATCCCCAAATGACCATGGTCTTTGGCGATGGGGACAATGATGTGGAAATTTTTAATGCTGTCAAGTACTCTGTCGCAATGGCTAACGGTAGTGATTTAGCAAAACAAAACGCGACCTTTATTTCGGAGTTTGATAATGACCATGATGGCATTTATCACTTTTTGCAATGCTTTCTAAAAATTGAGTAA", "aa": "MKPKVQNLIFDLDGTLLSWGHEPLPQTVTFLKELQKQGFKITFATGRSHILIRNTTQFIQPDLPVISSNGALIYDFAREKALHMTQLAPQSVVPIMRLLLQLEESFCIYTDKKVFGFEKPGIPCKRLRTTQSKIVEPDITQNNFTINPLTDASKFDFATQNITKILLITEDRGRISKITKHLDAIENISYVSSMTFALDIMHKDVNKAYGLKALEQQTGLDPQMTMVFGDGDNDVEIFNAVKYSVAMANGSDLAKQNATFISEFDNDHDGIYHFLQCFLKIE", "aa_hexdigest": "1ad92bee8a24b5640789e142d243a3bb", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000139536", "ncbi_nrp_id": "WP_010874739.1", "uniref100_id": "UniRef100_P75399", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139536", "RefSeq:WP_010874739.1", "UniRef:UniRef100_P75399" ] }, "ips": { "uniref100_id": "UniRef100_P75399", "uniref90_id": "UniRef90_P75399" }, "psc": { "uniref90_id": "UniRef90_P75399", "gene": "Cof", "product": "Putative phosphatase MPN_383", "ec_ids": [ "3.1.3.-" ], "uniref50_id": "UniRef50_P75399", "cog_id": "COG0561", "cog_category": "HR", "kegg_orthology_id": "K21064", "go_ids": [ "GO:0005829", "GO:0000287", "GO:0016791" ] }, "pscc": { "uniref50_id": "UniRef50_P75399", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75399" ], "product": "Putative phosphatase MPN_383" }, "genes": [ "cof" ], "id": "APKECPOBEN_833", "locus": "GCA002090215_00810" }, { "type": "cds", "sequence": "contig_1", "start": 460428, "stop": 460943, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08264.19" ], "nt": "GTGCACAATTTCTTTCACAAGGAAAACATTATTAAAGAAACGGTAGATCAAGAAACTGTTTATGGTTATAACCTCTTTTTAAAGCGTAGTTACGAGCACTTAGAGAAACAAGAACTCAATTTGGTAATTAGCCAAATGATGATCTTTTTAAACCTGCTTTACAAAACAAAACAATTAACCCTGGCTTATGCAAAGGGCTTTTTGACGGTGTTATCGTTTTTTGCACCTTATTTAGCGGAAGAATTAAACGCTAAACTAGGGATGGAGCCGTTTATTGTTCATGCTCAACTACCAAATGTTGATAACAGCGTACTTGAAACCGATAAGGTTAAAATTATTCTTTCGGTCAACGGTCGCTTCAAAGGTACCAAGGAATTTGCTAAAGGGGTTGATGAACAGACTGTCTTAAAAGCCTTTAAGACAGATCCGGAATTCCAAGCGTTGTTCGACCAACCCTTAGCGCGTGTTGTTTTTGTCCCTAATCGTATTATTAATGTTCTTTTAAAATCCGAATAG", "aa": "MHNFFHKENIIKETVDQETVYGYNLFLKRSYEHLEKQELNLVISQMMIFLNLLYKTKQLTLAYAKGFLTVLSFFAPYLAEELNAKLGMEPFIVHAQLPNVDNSVLETDKVKIILSVNGRFKGTKEFAKGVDEQTVLKAFKTDPEFQALFDQPLARVVFVPNRIINVLLKSE", "aa_hexdigest": "e305be601141d12026cba9449c168731", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08264.19", "name": "Anticodon_1", "length": 143, "aa_cov": 0.672514619883041, "hmm_cov": 0.993006993006993, "evalue": 3.3031489516385284e-12, "score": 41.97935104370117, "start": 20, "stop": 134 } ], "seq_stats": { "molecular_weight": 19760.796199999993, "isoelectric_point": 6.921022605895996 }, "id": "APKECPOBEN_834", "locus": "GCA002090215_00811" }, { "type": "cds", "sequence": "contig_1", "start": 460986, "stop": 461423, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00133.28" ], "nt": "GTGAAACAAGGCAATCAAACTTTTACTCGGGAAACGAGCACTATGCCCCAGTGGGCTGGTTCTTGTTGGTATTACTTGGGTTATTTGATGTTAATTAATAACGAAAACTTTTGGCCAATTGACTCCAGGGAAGCGAAAGATCTTTTTGAGCGTTACCTACCAGTGGATCTGTATGTGGGTGGTGCCGAACATGCCGTGTTGCACCTCTTGTACGCGCGCTTTTGGCACCAATTCTTGTACCAAAAGGGAATAGTCACCACTAAAGAGCCCTTTAAAAAGTTAATTAACCAGGGCATGGTATTGGGACCAGATGGCAAAAAGATGTCCAAATCCAAGGGCAACACAATCAACCCTACGCCACTAATTGATTCTCATGGTGCTGATGCCTTGCGACTTTACTTGATGTTTATGGGGCCGATTAGCGCGGCGTTGACCTGA", "aa": "MKQGNQTFTRETSTMPQWAGSCWYYLGYLMLINNENFWPIDSREAKDLFERYLPVDLYVGGAEHAVLHLLYARFWHQFLYQKGIVTTKEPFKKLINQGMVLGPDGKKMSKSKGNTINPTPLIDSHGADALRLYLMFMGPISAALT", "aa_hexdigest": "c54211d16e4a80300d5dfa2f49fd1912", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0012B0E3D9", "query_cov": 0.8551724137931035, "subject_cov": 0.9323308270676691, "identity": 0.629, "score": 155.0, "evalue": 2.23e-46, "valid": false, "product": "class I tRNA ligase family protein", "uniref50_id": "UniRef50_A0A359BCU9" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00133.28", "name": "tRNA-synt_1", "length": 137, "aa_cov": 0.9379310344827586, "hmm_cov": 0.8686131386861314, "evalue": 5.86608149964795e-20, "score": 66.81082153320312, "start": 4, "stop": 139 } ], "seq_stats": { "molecular_weight": 16596.034399999982, "isoelectric_point": 8.967697334289554 }, "id": "APKECPOBEN_835", "locus": "GCA002090215_00812" }, { "type": "cds", "sequence": "contig_1", "start": 461445, "stop": 462149, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00133.28", "PFAM:PF13603.12" ], "nt": "ATGCAAACCAACTGGATTGGTAAAAGTGAAGGGGTAGAGGTAACGTTTGACATAGAAAACTCCAAGGAAACGATTACCATTTTTACCACTAAGGTGCAAACTATTTTTGGTGTAACCTTTTTAGCGCTTTCCAATAGCCATCCCTTAGTAAAAGAGGTAGCTAAAACCAACCCCAAGATAGCACAGTTTCTCCAAAAACAAGCACAGAAAACCACCACTGTAAAGCAACCTGAGACGCTCCATGACGGGGTTGATTTAAAACTTAAAGCAATTAATCCAGCAACTAATACTGCCATTCCGTTGTATGTAGCTAACTATGTAGTGGAAGGTTATGGTACGGATGCTGTGATGGGTGTGCCAGCGCATAACGAAAACGATAACTTCTTCGCGCGGAAGCAAAAGCTACCGATTATTACCGTCATTGATAAACAGGAACGCTTGCAACACTCCGGGCAGTTTAGCGGGCTCAACTCCCAAACTGCTAACACCCAAATTACGCAAATGTTAGTCGAGCGTCAAAAAGCTAAAAAAACAACGGTGTACAAACTCCGTGACTGGATTTTTAGCCGTCAACGTTACTGGGGTGAACCTTTTCCGATTTTGTTTGACGAAAACAACCAACCCCATTTAGTTAAAGAGCTCCCCGTTACCCTACCAGCTTTGGCTAACTATCAACCTGATGGTTCGACTAACCCACCATTGTGA", "aa": "MQTNWIGKSEGVEVTFDIENSKETITIFTTKVQTIFGVTFLALSNSHPLVKEVAKTNPKIAQFLQKQAQKTTTVKQPETLHDGVDLKLKAINPATNTAIPLYVANYVVEGYGTDAVMGVPAHNENDNFFARKQKLPIITVIDKQERLQHSGQFSGLNSQTANTQITQMLVERQKAKKTTVYKLRDWIFSRQRYWGEPFPILFDENNQPHLVKELPVTLPALANYQPDGSTNPPL", "aa_hexdigest": "50d8eaae518275966a9d2fc6f010b21f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00133.28", "name": "tRNA-synt_1", "length": 39, "aa_cov": 0.16666666666666666, "hmm_cov": 1.0, "evalue": 1.5000351321885294e-07, "score": 25.940616607666016, "start": 170, "stop": 208 }, { "id": "PF13603.12", "name": "tRNA-synt_1_2", "length": 180, "aa_cov": 0.6965811965811965, "hmm_cov": 1.0, "evalue": 1.2944755563205112e-54, "score": 179.9636688232422, "start": 4, "stop": 166 } ], "seq_stats": { "molecular_weight": 26351.713100000004, "isoelectric_point": 8.942103385925293 }, "id": "APKECPOBEN_836", "locus": "GCA002090215_00813" }, { "type": "cds", "sequence": "contig_1", "start": 462168, "stop": 462365, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAAGGAGATTGATGTCAACTGGTGTGCTGAATTGGGCACCGTTTTAGCCAACGAAGAGGTGTTGATTGACAGTAATGGTAATGCGGTGAGTGAGCGTGGCGAGTTCCCGGTAACCAAGCGAAAAATGAAGCAATGGGTGTTGAAAATAACTGCTTTTGCTGAAAGTTTACTCAATGGTTTGGCTGAGCTGGATTGA", "aa": "MKEIDVNWCAELGTVLANEEVLIDSNGNAVSERGEFPVTKRKMKQWVLKITAFAESLLNGLAELD", "aa_hexdigest": "2ab89cd3ebffe9460b2bc0c35556143d", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7236.2179, "isoelectric_point": 4.67252902984619 }, "id": "APKECPOBEN_837", "locus": "GCA002090215_00814" }, { "type": "cds", "sequence": "contig_1", "start": 462516, "stop": 462701, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00133.28" ], "nt": "ATGTTCCCTTATCCTTCAGCGGCTGGTTTACACCTGGGACATGTGCGCGCTTACACCATTACCGATGTTTTGAGTCGTTATCATAAAGCGAAGGGTTTTAATGTGATTCACCCGATTGGCTTTGATGCTTTTGGGTTACCAGCAGAACAATACGCGATTGCCTCCAATAAGCATCCTGGTGACTGA", "aa": "MFPYPSAAGLHLGHVRAYTITDVLSRYHKAKGFNVIHPIGFDAFGLPAEQYAIASNKHPGD", "aa_hexdigest": "f22d21b9d83767ebec682861ab446d36", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00133.28", "name": "tRNA-synt_1", "length": 47, "aa_cov": 0.7540983606557377, "hmm_cov": 1.0, "evalue": 1.2225320232569379e-09, "score": 32.821102142333984, "start": 5, "stop": 50 } ], "seq_stats": { "molecular_weight": 6666.495200000001, "isoelectric_point": 8.155846977233889 }, "id": "APKECPOBEN_838", "locus": "GCA002090215_00815" }, { "type": "cds", "sequence": "contig_1", "start": 462810, "stop": 463154, "strand": "-", "frame": 2, "gene": null, "product": "MPN385 family protein", "db_xrefs": [ "GO:0005886", "RefSeq:WP_010874741.1", "SO:0001217", "UniParc:UPI000013953E", "UniRef:UniRef100_P75397", "UniRef:UniRef50_P47509", "UniRef:UniRef90_P75397" ], "nt": "ATGCTGCTTAAACGGCTCATTAAACTAGCGATTTTCCTCTTCTTTGTCGCGGTTGGTTTTATTATTTTTATTGGCTCTTTTTGGCTCAATACCTACAATACTAAGGAGTGGGCTAATTTACTAGCTGAAAAGGATGCTAGTGGGCTAATTGTGCAAATTATTCCCAATATTAATCAATGGTTTAAAGGCACTGTAGAGCAACAGCAAAAACTGTTCCAAACCCTGGTGCACTTCTTTATTCCAGTTGGTTTTGGTTTACTTTTTGGGATTGCTGTAGCCATTATAGCTGACCTGTTCTACCATTTAATTAAGTATTTAATTAAGCGCTCGTTCAAAAAAAACTAG", "aa": "MLLKRLIKLAIFLFFVAVGFIIFIGSFWLNTYNTKEWANLLAEKDASGLIVQIIPNINQWFKGTVEQQQKLFQTLVHFFIPVGFGLLFGIAVAIIADLFYHLIKYLIKRSFKKN", "aa_hexdigest": "b14793ed5c10d43955f82c9e34d384c2", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013953E", "ncbi_nrp_id": "WP_010874741.1", "uniref100_id": "UniRef100_P75397", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013953E", "RefSeq:WP_010874741.1", "UniRef:UniRef100_P75397" ] }, "ips": { "uniref100_id": "UniRef100_P75397", "uniref90_id": "UniRef90_P75397" }, "psc": { "uniref90_id": "UniRef90_P75397", "product": "MPN385 family protein", "uniref50_id": "UniRef50_P47509", "go_ids": [ "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47509", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47509" ], "product": "Uncharacterized protein MG267" }, "genes": [], "id": "APKECPOBEN_839", "locus": "GCA002090215_00816" }, { "type": "cds", "sequence": "contig_1", "start": 463189, "stop": 463557, "strand": "-", "frame": 1, "gene": "dck", "product": "putative MG268-like protein", "db_xrefs": [ "COG:COG1428", "COG:F", "GO:0005524", "GO:0005737", "GO:0019136", "SO:0001217", "UniRef:UniRef50_P75396", "UniRef:UniRef90_P75396" ], "nt": "TTGTTCGCCAAGCAAAACATCCACCGTCCTTCCATCTTTACTTACTATAACCACTTGTGGAATGGGATTGTTAAAGAACTAATCTTTAAACATGGTATTCCCGCTTTGTACGTTATCTTAGAAGGTGATTGGGAGTTGTTTGAACAAAGATTGTTTCAACGTAACCGTAAAGTAGAAATTGATAACTTTGCCAAAAATAAGGACTACTTTAAGAACCTGTACAAGATTTATGGTGAATTTATTAAAAACGTTTGTTATGACTTTGGTATTAGCCATTGCATAGTAAACGCTAACCAATCAGTAGAATCCATCACCAAACAAGTACTAGAGGTATTAAAGTCAAAAAACTTAGACTGGGAAATTATCTAG", "aa": "MFAKQNIHRPSIFTYYNHLWNGIVKELIFKHGIPALYVILEGDWELFEQRLFQRNRKVEIDNFAKNKDYFKNLYKIYGEFIKNVCYDFGISHCIVNANQSVESITKQVLEVLKSKNLDWEII", "aa_hexdigest": "a6fb0518e0e5df9168fc660f22ac9fea", "start_type": "TTG", "rbs_motif": null, "genes": [ "dck" ], "truncated": "3-prime", "pseudogene": { "start": 463189, "stop": 463857, "observations": { "mutation": [ 463560 ], "stop": [ 463560 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75396", "query_cov": 1.0, "subject_cov": 0.5327510917030568, "identity": 0.992, "score": 258.0, "evalue": 6.68e-86, "gene_start": 1, "gene_end": 122, "reference_start": 108, "reference_end": 229, "reference_sequence": "MKTPLKPKFQPAKIANAVVVGGMIAFGKTTIAESLAKHLKGSKVIYELEEQDQLADLLLAKMYERNDELLYAPLFQLYFTLNRFNKYRKECNNKTPTIFDRSIFEDWLFAKQNIHRPSIFTYYNHLWNGIVKELIFKHGIPALYVILEGDWELFEQRLFQRNRKVEIDNFAKNKDYFKNLYKIYGEFIKNVCYDFGISHCIVNANQSVESITKQVLEVLKSKNLDWEII" }, "paralog": false, "identity": 0.9954954954954955, "score": 449.0, "evalue": 1.03e-163, "description": "Internal stop codon at 463560. Nonsense mutation around 463560.", "psc": { "uniref90_id": "UniRef90_P75396", "gene": "Dck", "product": "Uncharacterized protein MG268 homolog", "uniref50_id": "UniRef50_P75396", "cog_id": "COG1428", "cog_category": "F", "go_ids": [ "GO:0005737", "GO:0005524", "GO:0019136" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75396", "UniRef:UniRef50_P75396", "COG:COG1428", "COG:F", "GO:0005737", "GO:0005524", "GO:0019136" ] }, "pscc": { "uniref50_id": "UniRef50_P75396", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75396" ], "product": "Uncharacterized protein MG268 homolog" } }, "id": "APKECPOBEN_840", "locus": "GCA002090215_00817" }, { "type": "cds", "sequence": "contig_1", "start": 463558, "stop": 463878, "strand": "-", "frame": 1, "gene": "dck", "product": "putative MG268-like protein", "db_xrefs": [ "COG:COG1428", "COG:F", "GO:0005524", "GO:0005737", "GO:0019136", "SO:0001217", "UniRef:UniRef50_P75396", "UniRef:UniRef90_P75396" ], "nt": "ATGAAGACTCCCTTAAAACCCAAATTTCAACCAGCTAAGATTGCTAACGCCGTTGTGGTTGGTGGCATGATTGCTTTTGGTAAAACGACGATAGCTGAAAGTTTAGCTAAACACTTAAAGGGTTCTAAGGTGATTTACGAACTGGAAGAACAAGACCAGCTAGCTGATTTATTGTTAGCCAAAATGTATGAGCGTAACGACGAGTTGCTCTACGCCCCTTTATTTCAACTGTACTTTACCTTAAACCGCTTTAATAAATACCGCAAGGAATGTAACAATAAAACACCAACTATCTTTGACCGCTCGATCTTTGAAGATTGA", "aa": "MKTPLKPKFQPAKIANAVVVGGMIAFGKTTIAESLAKHLKGSKVIYELEEQDQLADLLLAKMYERNDELLYAPLFQLYFTLNRFNKYRKECNNKTPTIFDRSIFED", "aa_hexdigest": "0105850491dd30c47ea318e0d7109897", "start_type": "ATG", "rbs_motif": null, "genes": [ "dck" ], "truncated": "5-prime", "pseudogene": { "start": 463255, "stop": 463878, "observations": { "mutation": [ 463560 ], "stop": [ 463560 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75396", "query_cov": 1.0, "subject_cov": 0.462882096069869, "identity": 1.0, "score": 214.0, "evalue": 5.33e-69, "gene_start": 1, "gene_end": 106, "reference_start": 1, "reference_end": 106, "reference_sequence": "MKTPLKPKFQPAKIANAVVVGGMIAFGKTTIAESLAKHLKGSKVIYELEEQDQLADLLLAKMYERNDELLYAPLFQLYFTLNRFNKYRKECNNKTPTIFDRSIFEDWLFAKQNIHRPSIFTYYNHLWNGIVKELIFKHGIPALYVILEGDWELFEQRLFQRNRKVEIDNFAKNKDYFKNLYKIYGEFIKNVCYDFGISHCIVNANQSVESITKQVLEVLKSKNLDWEII" }, "paralog": false, "identity": 0.9951690821256038, "score": 423.0, "evalue": 1.78e-153, "description": "Internal stop codon at 463560. Nonsense mutation around 463560.", "psc": { "uniref90_id": "UniRef90_P75396", "gene": "Dck", "product": "Uncharacterized protein MG268 homolog", "uniref50_id": "UniRef50_P75396", "cog_id": "COG1428", "cog_category": "F", "go_ids": [ "GO:0005737", "GO:0005524", "GO:0019136" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75396", "UniRef:UniRef50_P75396", "COG:COG1428", "COG:F", "GO:0005737", "GO:0005524", "GO:0019136" ] }, "pscc": { "uniref50_id": "UniRef50_P75396", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75396" ], "product": "Uncharacterized protein MG268 homolog" } }, "id": "APKECPOBEN_841", "locus": "GCA002090215_00818" }, { "type": "ncRNA", "class": null, "sequence": "contig_1", "start": 463912, "stop": 464007, "strand": "-", "gene": "Bacteria_small_SRP", "product": "Bacterial small signal recognition particle RNA", "score": 60.8, "evalue": 1.1e-07, "db_xrefs": [ "RFAM:RF00169", "SO:0000655" ], "nt": "ATTTAGAGCCGTCACATCATTACGGTCGAATCATGTCAGGCCAGAAATGGAGCAGCATTAAGACTATTTAATGAGTGTGATGGTTTTTTATTGCAT", "id": "APKECPOBEN_43", "locus": "GCA002090215_00819" }, { "type": "cds", "sequence": "contig_1", "start": 464026, "stop": 465102, "strand": "-", "frame": 1, "gene": null, "product": "putative MG269-like protein", "db_xrefs": [ "RefSeq:WP_010874743.1", "SO:0001217", "UniParc:UPI0000139546", "UniRef:UniRef100_P75395", "UniRef:UniRef50_Q49407", "UniRef:UniRef90_P75395" ], "nt": "ATGACTAACTTTGAGTATTACCGTGATTTTGATGACTTTCAAAGAAGAGAGACAATTAACTTCTTTCTTTCAAAGTTTCCACTAGCGAGTCAAAAACAGCTCCAGGACTTCCTAGAGCAAGCGCGTCAGGCTTATGTGCAGTTACGCCAAACCAACCCTGCACACCTTGACTGGAACCAAACATTGCTGTATTTAGCACAGAAATTTCTCCCCGAAAAGCAATCAGAGAAGGATCGCTTAAAGAAGATCTTGGTGTTGCAAGAACAGCTCAAAGTACGGTATGAAGGGGAAATTAAACGCCAGAGCCAACAAAACAGTGAGCTGTTAATCCAGTTAGGTCAACGTGATGAGGAAATTATCCAGATGCAGCAGCTATTTAAAGAAAAGGAACGGCAACTAGAGGTGTATCAAAAGCAGCTCAATGAAGCCAAGGAGTACAACCACAAGCTTGAGGAACACTACAACAAAACCCTGGAAGAAGCGTTGAAGGAGTACGAGCAGCAGTGTACTGATGCCATTCACAGGCGTGATGAGGAAATCCAGGCCATCTTTACAAGTAAGTTAAACGAAAAGAACAGTGAGATTACCCAACTGCAAACTTACTTGCAAAGTGCGGTTGATGAAAATGAGGCGTTGCAAAAGCAGCACAAACTAGTGTTGTTTAAAAACCAAAAGTACGAAAAGATGGTGAGTGACTTGCAAGTCGATTTAGCCCGGATTCAGGAAATTAACAATTCACTTACCAGTGAAAAGCGTGACTTTCAGCGCGCTAACAACGATTTGGTCAAGCAGTACAACAAACTCAAAAACCGCTTGGAACAAAAGTTAGGTGAAATTACTAACGCGCAAGTTAATGGTCAGACCCATACCGTTAGCCTAGCTGACACTTCACAGCAATTCCACCGTCCTCAAGAGGCTGTTATCCCGCAAACCCAAGTTATTAGCTATACACTCGATGATATGGATGATGACATGGAAGTGGAAGAAACTCCACCAACAACCAATAAAGATCTACCTCGCGGCGCGACCCAGCCGAAGCGTAATTCGATTAAGCGCGTAAGCAAATTAATTGATTAA", "aa": "MTNFEYYRDFDDFQRRETINFFLSKFPLASQKQLQDFLEQARQAYVQLRQTNPAHLDWNQTLLYLAQKFLPEKQSEKDRLKKILVLQEQLKVRYEGEIKRQSQQNSELLIQLGQRDEEIIQMQQLFKEKERQLEVYQKQLNEAKEYNHKLEEHYNKTLEEALKEYEQQCTDAIHRRDEEIQAIFTSKLNEKNSEITQLQTYLQSAVDENEALQKQHKLVLFKNQKYEKMVSDLQVDLARIQEINNSLTSEKRDFQRANNDLVKQYNKLKNRLEQKLGEITNAQVNGQTHTVSLADTSQQFHRPQEAVIPQTQVISYTLDDMDDDMEVEETPPTTNKDLPRGATQPKRNSIKRVSKLID", "aa_hexdigest": "e68b6232f2acd9e744b6f05bfa25e0e0", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000139546", "ncbi_nrp_id": "WP_010874743.1", "uniref100_id": "UniRef100_P75395", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139546", "RefSeq:WP_010874743.1", "UniRef:UniRef100_P75395" ] }, "ips": { "uniref100_id": "UniRef100_P75395", "uniref90_id": "UniRef90_P75395" }, "psc": { "uniref90_id": "UniRef90_P75395", "product": "Uncharacterized protein MG269 homolog", "uniref50_id": "UniRef50_Q49407" }, "pscc": { "uniref50_id": "UniRef50_Q49407", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49407" ], "product": "Uncharacterized protein MG269" }, "genes": [], "id": "APKECPOBEN_842", "locus": "GCA002090215_00820" }, { "type": "cds", "sequence": "contig_1", "start": 465095, "stop": 465481, "strand": "-", "frame": 3, "gene": null, "product": "putative MG269-1-like protein", "db_xrefs": [ "RefSeq:WP_015344909.1", "SO:0001217", "UniParc:UPI000013954B", "UniRef:UniRef100_Q9EXD4", "UniRef:UniRef50_Q9EXD4", "UniRef:UniRef90_Q9EXD4" ], "nt": "ATGAGTAACTTCTTTGAGAAATACATTAACGGTTTTATTGAAACTTTAGACCAAATTGACGCAGCTGACTTTCAACGCATCCAACACGACTTTGACCCTAACCAGTTTCCGTATGACTGGGTGGTGGAACGGGTTAGTGATGTCAAGGATTATTTGCTCAATCCGCGTGATTTTAGCGATGTAGAAACTTTTAAAAGTACTATGCGTGCTAAGATTAAGCACTTTTATGCGTGTTATTCGAGCAAAATCCCCTTCTTTTTGTTTACTAGTTTTGTGTTAGCAATCTTTAACAGTGTTGGGCAGTATGTCAAGTACCACTGTGACTTGGACTTTACTAATCCTGATGCTGTAACTATCTTTTTCCGTGAAAAGGCCTTAAATGACTAA", "aa": "MSNFFEKYINGFIETLDQIDAADFQRIQHDFDPNQFPYDWVVERVSDVKDYLLNPRDFSDVETFKSTMRAKIKHFYACYSSKIPFFLFTSFVLAIFNSVGQYVKYHCDLDFTNPDAVTIFFREKALND", "aa_hexdigest": "9c3ded2ea07bbf7121123b7e1a4ae6ae", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013954B", "ncbi_nrp_id": "WP_015344909.1", "uniref100_id": "UniRef100_Q9EXD4", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013954B", "RefSeq:WP_015344909.1", "UniRef:UniRef100_Q9EXD4" ] }, "ips": { "uniref100_id": "UniRef100_Q9EXD4", "uniref90_id": "UniRef90_Q9EXD4" }, "psc": { "uniref90_id": "UniRef90_Q9EXD4", "product": "Uncharacterized protein MG269.1 homolog", "uniref50_id": "UniRef50_Q9EXD4" }, "pscc": { "uniref50_id": "UniRef50_Q9EXD4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9EXD4" ], "product": "Uncharacterized protein MG269.1 homolog" }, "genes": [], "id": "APKECPOBEN_843", "locus": "GCA002090215_00821" }, { "type": "cds", "sequence": "contig_1", "start": 465474, "stop": 465695, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF10437.14" ], "nt": "GTGCAGGTAGATCAGGGTAAAGTAGTCGACATTAAGTTCTATGGGGACTTTTTGAGTGTTATCGACATTACCCCCGTTACCCAGCAGTTGGTAGGACAAAAATACGATTACCAAACCTTTGCTAAAATATTGGGTAACATTGATAACTTTAAGGAGTACTTTGGTACCTTAACACCTCAACAAATGCTCGAAGTTATCTTTGATAACAAAAAGGATGAGTAA", "aa": "MQVDQGKVVDIKFYGDFLSVIDITPVTQQLVGQKYDYQTFAKILGNIDNFKEYFGTLTPQQMLEVIFDNKKDE", "aa_hexdigest": "24bc7a293f1e49b285be31c717c6d482", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF10437.14", "name": "Lip_prot_lig_C", "length": 66, "aa_cov": 0.9041095890410958, "hmm_cov": 0.9848484848484849, "evalue": 4.513729399101965e-18, "score": 60.497222900390625, "start": 1, "stop": 66 } ], "seq_stats": { "molecular_weight": 8470.543799999998, "isoelectric_point": 4.525259208679199 }, "id": "APKECPOBEN_844", "locus": "GCA002090215_00822" }, { "type": "cds", "sequence": "contig_1", "start": 465771, "stop": 466493, "strand": "-", "frame": 2, "gene": "lplA", "product": "putative lipoate-protein ligase A", "db_xrefs": [ "COG:COG0095", "COG:H", "EC:6.3.1.20", "GO:0005524", "GO:0005737", "GO:0016979", "GO:0017118", "KEGG:K03800", "SO:0001217", "UniRef:UniRef50_P47512", "UniRef:UniRef90_P75394" ], "nt": "ATGAAAACTTACATTCTCACTTCCCCTAAAAACATTCCTTACTTCAACGCAGCGTTAGAAGAATGGCTACTCACCGAATTTAAAAAGGGCGAGGAAATTAAAGTCATTTACTTCTGGCAAAACGCCAACACGATTGTGGTGGGGCGCAACCAAAATACGTACGCTGAGGTTAACCTTAGTGAAGTGGAAAAGGACAAGGTTAATTTGTTTCGCCGTTTTTCTGGAGGTGGGGCGGTGTTTCATGACATGGGTAACATCTGTTTCTCAATTATTTTGCCCAAAGCAAAAAAGGAAATGGAAAACGCTTATGAAGAAACAACCCGTAATGTGGTGAAGTTCTTAAACAGTGTAGGTGTACCCGCACAGTTTCATGGCCGCAATGACTTGGAAATTGAGGGCAAGAAGTTCTCTGGTTTAGCGGAATACCTCTCCAAAGACCGCGTGTTGGTCCACGGTACGTTGTTGTTTGATACCGACTTTACCAAGCTAGCGAAGTACCTCAATGTGGATAAAACAAAAATGGTAAGTAAGGGAATTGAAAGTGTCCAAAAACGGGTAGTTAACGTAAAAGAATACCTCCCCAACCTCTCTACCCCAACCTTCTTGGAAAAGATGGTGCAGTTCTTTACCGAAACCGAACATGCTGAAACGATTCATTTGGATGAAAGCAGCATTAAGATGGTGGAAAAACGTGCACAGGAACACTTCCAATCCTGGGATTGA", "aa": "MKTYILTSPKNIPYFNAALEEWLLTEFKKGEEIKVIYFWQNANTIVVGRNQNTYAEVNLSEVEKDKVNLFRRFSGGGAVFHDMGNICFSIILPKAKKEMENAYEETTRNVVKFLNSVGVPAQFHGRNDLEIEGKKFSGLAEYLSKDRVLVHGTLLFDTDFTKLAKYLNVDKTKMVSKGIESVQKRVVNVKEYLPNLSTPTFLEKMVQFFTETEHAETIHLDESSIKMVEKRAQEHFQSWD", "aa_hexdigest": "35fae1dcbde01bb47762ea6eb37ab1c9", "start_type": "ATG", "rbs_motif": null, "genes": [ "lplA" ], "truncated": "5-prime", "pseudogene": { "start": 465474, "stop": 466493, "observations": { "mutation": [ 465773 ], "stop": [ 465773 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75394", "query_cov": 1.0, "subject_cov": 0.7079646017699115, "identity": 1.0, "score": 478.0, "evalue": 3.07e-169, "gene_start": 1, "gene_end": 240, "reference_start": 1, "reference_end": 240, "reference_sequence": "MKTYILTSPKNIPYFNAALEEWLLTEFKKGEEIKVIYFWQNANTIVVGRNQNTYAEVNLSEVEKDKVNLFRRFSGGGAVFHDMGNICFSIILPKAKKEMENAYEETTRNVVKFLNSVGVPAQFHGRNDLEIEGKKFSGLAEYLSKDRVLVHGTLLFDTDFTKLAKYLNVDKTKMVSKGIESVQKRVVNVKEYLPNLSTPTFLEKMVQFFTETEHAETIHLDESSIKMVEKRAQEHFQSWDWNFGKTADYNFKNKKRFEGAGIFECNVQVDQGKVVDIKFYGDFLSVIDITPVTQQLVGQKYDYQTFAKILGNIDNFKEYFGTLTPQQMLEVIFDNKKDE" }, "paralog": false, "identity": 0.9970501474926253, "score": 681.0, "evalue": 1.72e-251, "description": "Internal stop codon at 465773. Nonsense mutation around 465773.", "psc": { "uniref90_id": "UniRef90_P75394", "gene": "lplA", "product": "Probable lipoate-protein ligase A", "ec_ids": [ "6.3.1.20" ], "uniref50_id": "UniRef50_P47512", "cog_id": "COG0095", "cog_category": "H", "kegg_orthology_id": "K03800", "go_ids": [ "GO:0005737", "GO:0005524", "GO:0016979", "GO:0017118" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75394", "EC:6.3.1.20", "UniRef:UniRef50_P47512", "COG:COG0095", "COG:H", "KEGG:K03800", "GO:0005737", "GO:0005524", "GO:0016979", "GO:0017118" ] }, "pscc": { "uniref50_id": "UniRef50_P47512", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47512" ], "product": "Probable lipoate-protein ligase A" } }, "id": "APKECPOBEN_845", "locus": "GCA002090215_00823" }, { "type": "cds", "sequence": "contig_1", "start": 466520, "stop": 467650, "strand": "-", "frame": 3, "gene": "lpdA", "product": "dihydrolipoyl dehydrogenase", "db_xrefs": [ "COG:C", "COG:COG1249", "EC:1.8.1.4", "GO:0004148", "GO:0005829", "GO:0006090", "GO:0006103", "GO:0016020", "GO:0050660", "KEGG:K00382", "SO:0001217", "UniRef:UniRef50_P75393", "UniRef:UniRef90_P75393" ], "nt": "TTGCTGGAGCAAAAAGGTAAGGTAGTGAGTAAGTTAGTTGGTGGGGTTAAAGCGATTATTGCTTCCGCTAAAGCTGAAACGGTAATGGGAGAAGCCAAGGTTTTGGATCCCAACACCGTGGAGGTAGCGGGTAAAACTTACACCACCAAAAGCATTGTGGTCGCAACTGGTTCACGACCCCGTTACTTAACGCTTCCGGGTTTTGCAGAAGCGCGTCAAAACGGTTTTGTAATTGACTCGACCCAAGCGCTCTCTTTGGAAGGGGTACCACGCAAGTTAGTTGTTGTTGGTGGTGGAGTGATTGGTATTGAATTTGCCTTTCTCTACGCTTCATTGGGTAGTGAAGTGACTATCTTACAAGGGGTGGATAGGATCTTGGAAATCTTTGACACTGAGGTATCAGATTTAGTCGCTAAACTCTTGCAAACCAAGAATGTCAAGATTATTACCAACGCACAGGTAACCCGTGCTAACAATAATGAAGTCTTTTATTCGCAAAACGGGCAGGAAGGCAGTGTGGTTGGTGATCGCATTTTGGTCTCGATTGGTCGCATTCCCAACACGGAATGTCTTGATGGGTTGAACTTACAACGTGATGAGCGCAACCGGATTGTGCTCAATCAAGATCTCCAAACCTCAATTCCCAACATCTACATAGTTGGCGATGCCAATGCCCAGTTAATGTTGGCCCACTTTGCCTACCAACAAGGGCGGTATGCGGTGAACCATATCTTAAACAAGAAGCAGGTCAAACCAGCGCAAAAGTTAACTTGCCCTTCCTGTATTTACACCAATCCGGAAGTAGCATCGGTGGGTTACACCGAAATGGAGTTGAAAAAACAGGGCATTCCTTATGTCAAAACCAATCTTGTTTTAGCGCACTGTGGAAAAGCAATCGCTGACAATGAAACCAACGGTTTTGTCAAAATGATGTTTGACCCACAAACCGGTAAGATCTTGGGTTGCTGTATTATTGCTGCTACTGCTAGTGACATGATTGCCGAGCTAGCGTTGGCTATGGGTGCAGGTTTGACAGTATTTGACATTGCCAACTCCATTTCACCACATCCTACCATCAACGAAATGATCGCTGATGTGTGCAAAAAAGCTCTTTTTGATCACTTTAAATAA", "aa": "MLEQKGKVVSKLVGGVKAIIASAKAETVMGEAKVLDPNTVEVAGKTYTTKSIVVATGSRPRYLTLPGFAEARQNGFVIDSTQALSLEGVPRKLVVVGGGVIGIEFAFLYASLGSEVTILQGVDRILEIFDTEVSDLVAKLLQTKNVKIITNAQVTRANNNEVFYSQNGQEGSVVGDRILVSIGRIPNTECLDGLNLQRDERNRIVLNQDLQTSIPNIYIVGDANAQLMLAHFAYQQGRYAVNHILNKKQVKPAQKLTCPSCIYTNPEVASVGYTEMELKKQGIPYVKTNLVLAHCGKAIADNETNGFVKMMFDPQTGKILGCCIIAATASDMIAELALAMGAGLTVFDIANSISPHPTINEMIADVCKKALFDHFK", "aa_hexdigest": "702a4d311be2f2d4f0b8eea0bdf4f04c", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75393", "query_cov": 1.0, "subject_cov": 0.8209606986899564, "identity": 0.992, "score": 720.0, "evalue": 1.83e-260, "valid": true, "gene": "lpdA", "product": "dihydrolipoyl dehydrogenase", "ec_ids": [ "1.8.1.4" ], "uniref50_id": "UniRef50_P75393", "cog_id": "COG1249", "cog_category": "C", "kegg_orthology_id": "K00382", "go_ids": [ "GO:0004148", "GO:0005829", "GO:0006090", "GO:0006103", "GO:0016020", "GO:0050660" ] }, "pscc": { "uniref50_id": "UniRef50_P75393", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75393" ], "product": "Dihydrolipoyl dehydrogenase" }, "genes": [ "lpdA" ], "id": "APKECPOBEN_846", "locus": "GCA002090215_00824" }, { "type": "cds", "sequence": "contig_1", "start": 467657, "stop": 467893, "strand": "-", "frame": 3, "gene": null, "product": "FAD-dependent oxidoreductase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7X1ZRV2" ], "nt": "ATGAATTACGATCTCATTATTATAGGGGCTGGTCCCGCTGGGTATGTGGCCGCTGAGTACGCGGGTAAGCATAAACTTAAAACACTCGTTGTCGAAAAGGAATACTTTGGTGGCGTGTGTTTAAATGTTGGTTGTATTCCAACGAAAACACTGTTAAAACGGGCCAAGATTGTCGATTACCTCCGCCATGCGCAGGACTATGGTATTTCAATTAATGGTCAAGTAGCACTTAACTGA", "aa": "MNYDLIIIGAGPAGYVAAEYAGKHKLKTLVVEKEYFGGVCLNVGCIPTKTLLKRAKIVDYLRHAQDYGISINGQVALN", "aa_hexdigest": "c60591b31d3004a81de8c260af0c4654", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0010C3BE0E", "query_cov": 0.9358974358974359, "subject_cov": 0.9358974358974359, "identity": 0.589, "score": 100.0, "evalue": 1.85e-26, "valid": false, "product": "FAD-dependent oxidoreductase", "uniref50_id": "UniRef50_A0A7X1ZRV2" }, "pscc": { "uniref50_id": "UniRef50_A0A7X1ZRV2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7X1ZRV2" ], "product": "FAD-dependent oxidoreductase" }, "genes": [], "id": "APKECPOBEN_847", "locus": "GCA002090215_00825" }, { "type": "cds", "sequence": "contig_1", "start": 467894, "stop": 469102, "strand": "-", "frame": 3, "gene": "pdhC", "product": "Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex", "db_xrefs": [ "COG:C", "COG:COG0508", "EC:2.3.1.12", "GO:0004742", "GO:0005829", "GO:0009986", "GO:0016020", "GO:0031405", "RefSeq:WP_010874747.1", "SO:0001217", "UniParc:UPI0000130BFE", "UniRef:UniRef100_P75392", "UniRef:UniRef50_P75392", "UniRef:UniRef90_P75392" ], "nt": "ATGGCAAATGAGTTTAAGTTCACCGATGTTGGTGAGGGCTTACATGAGGGTAAAGTAACTGAAATCCTCAAAAAAGTGGGCGATACGATCAAGGTCGATGAAGCCCTTTTTGTAGTGGAAACCGATAAGGTAACCACCGAACTTCCATCCCCTTATGCTGGGGTTATTACCGCCATTACCACTAATGTTGGTGATGTGGTGCACATTGGTCAGGTGATGGCCGTTATTGATGATGGCGCTGGTGCTGCTGCACCTGCTGCGCCCCAACCAGTATCTGCACCAGCTCCTGCCCCAACCCCAACATTTACCCCAACACCAGCACCTGTAACCACCGAACCAGTAGTGGAAGAGGCGGGTGCCTCGGTAGTGGGTGAAATTAAGGTATCGAACAGTGTGTTTCCTATTTTTGGTGTGCAGCCAAGTGCGCCCCAACCAACACCAGCTCCTGTAGTACAACCTACATCCGCTCCAACTCCCACTCCAGCTCCAGCATCTGCAGCAGCACCTAGTGGTGAGGAAACGATTGCGATCACGACAATGCGTAAAGCAATTGCTGAAGCAATGGTGAAATCACACGAAAACATTCCCGCTACGATTCTCACCTTCTATGTTAACGCTACTAAGTTAAAGCAGTACCGTGAGAGTGTTAACGGCTTAGCTTTAAGCAAGTACAACATGAAGATTTCCTTCTTTGCTTTCTTTGTTAAAGCAATTGTTAATGCTTTGAAGAAGTTTCCTGTTTTCAATGGTCGTTATGACAAGGAACGCAACCTAATTGTGTTAAACAAGGATGTTAACGTAGGTATTGCCGTGGACACTCCCGATGGTCTGATTGTGCCTAACATTAAGCAAGCACAAACGAAGTCGGTAGTCGACATTGCCAAGGACATTGTTGATCTTGCTAACCGCGCCCGCAGTAAGCAAATTAAGCTTCCTGATTTAAGTAAGGGAACGATCTCGGTAACTAACTTTGGTTCTTTAGGCGCTGCTTTTGGTACCCCGATTATTAAGCACCCGGAAATGTGTATTGTCGCTACCGGTAACATGGAAGAACGTGTTGTGCGTGCTGAAGGTGGGGTAGCCGTTCATACCATCCTACCGTTAACAATTGCTGCCGACCACCGCTGGGTCGATGGCGCTGATGTGGGTCGCTTTGGTAAGGAAATTGCCAAGCAAATCGAAGAATTAATTGATTTAGAAGTAGCTTAG", "aa": "MANEFKFTDVGEGLHEGKVTEILKKVGDTIKVDEALFVVETDKVTTELPSPYAGVITAITTNVGDVVHIGQVMAVIDDGAGAAAPAAPQPVSAPAPAPTPTFTPTPAPVTTEPVVEEAGASVVGEIKVSNSVFPIFGVQPSAPQPTPAPVVQPTSAPTPTPAPASAAAPSGEETIAITTMRKAIAEAMVKSHENIPATILTFYVNATKLKQYRESVNGLALSKYNMKISFFAFFVKAIVNALKKFPVFNGRYDKERNLIVLNKDVNVGIAVDTPDGLIVPNIKQAQTKSVVDIAKDIVDLANRARSKQIKLPDLSKGTISVTNFGSLGAAFGTPIIKHPEMCIVATGNMEERVVRAEGGVAVHTILPLTIAADHRWVDGADVGRFGKEIAKQIEELIDLEVA", "aa_hexdigest": "eb234ec07be3b945730db8634bb71aab", "start_type": "ATG", "rbs_motif": "AAAA", "ups": { "uniparc_id": "UPI0000130BFE", "ncbi_nrp_id": "WP_010874747.1", "uniref100_id": "UniRef100_P75392", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000130BFE", "RefSeq:WP_010874747.1", "UniRef:UniRef100_P75392" ] }, "ips": { "uniref100_id": "UniRef100_P75392", "uniref90_id": "UniRef90_P75392" }, "psc": { "uniref90_id": "UniRef90_P75392", "gene": "pdhC", "product": "Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex", "ec_ids": [ "2.3.1.12" ], "uniref50_id": "UniRef50_P75392", "cog_id": "COG0508", "cog_category": "C", "go_ids": [ "GO:0009986", "GO:0005829", "GO:0016020", "GO:0004742", "GO:0031405" ] }, "pscc": { "uniref50_id": "UniRef50_P75392", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75392" ], "product": "Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex" }, "genes": [ "pdhC" ], "id": "APKECPOBEN_848", "locus": "GCA002090215_00826" }, { "type": "cds", "sequence": "contig_1", "start": 469421, "stop": 469657, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02780.25" ], "nt": "GTGTTTAACTCCGTGAAGAAAACCGGGCGCTTGCTCGTAGTTACCGAAGCGGCTAAAACCTTTACCACCAGTGGGGAAATTATTGCATCGGTAACTGAAGAATTATTTAGCTATCTCAAGGCAGCCCCACAACGGGTAACCGGTTGGGACATTGTGGTACCGTTAGCACGGGGTGAACACTACCAGTTCAATTTAAATGCCCGCATTTTGGAAGCTGTTAACCAACTTTTAAAGTAA", "aa": "MFNSVKKTGRLLVVTEAAKTFTTSGEIIASVTEELFSYLKAAPQRVTGWDIVVPLARGEHYQFNLNARILEAVNQLLK", "aa_hexdigest": "42e4b34466ce553931a8307c3bb2b9f6", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02780.25", "name": "Transketolase_C", "length": 60, "aa_cov": 0.7692307692307693, "hmm_cov": 1.0, "evalue": 1.510524915594347e-13, "score": 46.22168731689453, "start": 3, "stop": 62 } ], "seq_stats": { "molecular_weight": 8694.967899999996, "isoelectric_point": 9.221831703186034 }, "id": "APKECPOBEN_849", "locus": "GCA002090215_00827" }, { "type": "cds", "sequence": "contig_1", "start": 469670, "stop": 470404, "strand": "-", "frame": 3, "gene": "pdhB", "product": "Pyruvate dehydrogenase E1 component subunit beta", "db_xrefs": [ "COG:C", "COG:COG0022", "EC:1.2.4.1", "GO:0001968", "GO:0004739", "GO:0005829", "GO:0009897", "GO:0009986", "GO:0016020", "GO:0031639", "GO:0033111", "GO:0051919", "SO:0001217", "UniRef:UniRef50_P75391", "UniRef:UniRef90_P75391" ], "nt": "ATGTCAAAAACAATTCAAGCAAATAACATTGAAGCGTTGGGTAACGCAATGGATCTAGCGTTAGAGCGCGACCCCAACGTTGTTTTATACGGTCAGGATGCTGGCTTTGAAGGTGGTGTGTTCCGTGCTACTAAGGGTTTACAAAAGAAGTACGGTGAGGAGCGTGTTTGGGACTGTCCGATTGCCGAAGCAGCTATGGCTGGTATTGGTGTGGGTGCTGCCATTGGTGGACTTAAACCAATCGTGGAAATCCAGTTCTCCGGTTTTTCTTTCCCCGCAATGTTCCAAATTTTTACCCACGCAGCACGGATCCGCAACCGTTCTCGCGGTGTGTATACCTGTCCGATTATTGTCCGCATGCCAATGGGTGGTGGGATTAAAGCACTTGAACACCACAGTGAGACCTTGGAAGCGATTTACGGTCAAATTGCCGGACTTAAAACAGTGATGCCGTCCAACCCTTACGACACCAAGGGCTTATTCTTAGCAGCAGTGGAATCACCTGACCCTGTGGTCTTCTTTGAACCAAAGAAGCTGTACCGTGCCTTCCGTCAGGAAATTCCAGCAGATTATTACACTGTGCCAATTGGTCAAGCTAACTTAATTAGCCAGGGTAACAACCTTACTATTGTTAGTTACGGTCCTACCATGTTTGACCTAATTAACATGGTCTATGGGGGTGAGTTGAAGGACAAGGGCATTGAACTCATTGACTTGCGTACCATTTCCCCTTGA", "aa": "MSKTIQANNIEALGNAMDLALERDPNVVLYGQDAGFEGGVFRATKGLQKKYGEERVWDCPIAEAAMAGIGVGAAIGGLKPIVEIQFSGFSFPAMFQIFTHAARIRNRSRGVYTCPIIVRMPMGGGIKALEHHSETLEAIYGQIAGLKTVMPSNPYDTKGLFLAAVESPDPVVFFEPKKLYRAFRQEIPADYYTVPIGQANLISQGNNLTIVSYGPTMFDLINMVYGGELKDKGIELIDLRTISP", "aa_hexdigest": "60542a124bde51fe8466edcf9360fd6d", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002FDA3C09", "query_cov": 0.930327868852459, "subject_cov": 0.9700854700854701, "identity": 0.555, "score": 267.0, "evalue": 3.08e-87, "valid": false, "product": "transketolase C-terminal domain-containing protein", "uniref50_id": "UniRef50_A0A537VMS1" }, "genes": [ "pdhB" ], "truncated": "5-prime", "pseudogene": { "start": 469421, "stop": 470404, "observations": { "mutation": [ 469672 ], "stop": [ 469672 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75391", "query_cov": 1.0, "subject_cov": 0.746177370030581, "identity": 1.0, "score": 490.0, "evalue": 4.45e-174, "gene_start": 1, "gene_end": 244, "reference_start": 1, "reference_end": 244, "reference_sequence": "MSKTIQANNIEALGNAMDLALERDPNVVLYGQDAGFEGGVFRATKGLQKKYGEERVWDCPIAEAAMAGIGVGAAIGGLKPIVEIQFSGFSFPAMFQIFTHAARIRNRSRGVYTCPIIVRMPMGGGIKALEHHSETLEAIYGQIAGLKTVMPSNPYDTKGLFLAAVESPDPVVFFEPKKLYRAFRQEIPADYYTVPIGQANLISQGNNLTIVSYGPTMFDLINMVYGGELKDKGIELIDLRTISPWDKETVFNSVKKTGRLLVVTEAAKTFTTSGEIIASVTEELFSYLKAAPQRVTGWDIVVPLARGEHYQFNLNARILEAVNQLLK" }, "paralog": false, "identity": 0.9969418960244648, "score": 646.0, "evalue": 9.28e-238, "description": "Internal stop codon at 469672. Nonsense mutation around 469672.", "psc": { "uniref90_id": "UniRef90_P75391", "gene": "pdhB", "product": "Pyruvate dehydrogenase E1 component subunit beta", "ec_ids": [ "1.2.4.1" ], "uniref50_id": "UniRef50_P75391", "cog_id": "COG0022", "cog_category": "C", "go_ids": [ "GO:0033111", "GO:0009986", "GO:0005829", "GO:0009897", "GO:0016020", "GO:0001968", "GO:0004739", "GO:0031639", "GO:0051919" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75391", "EC:1.2.4.1", "UniRef:UniRef50_P75391", "COG:COG0022", "COG:C", "GO:0033111", "GO:0009986", "GO:0005829", "GO:0009897", "GO:0016020", "GO:0001968", "GO:0004739", "GO:0031639", "GO:0051919" ] }, "pscc": { "uniref50_id": "UniRef50_P75391", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75391" ], "product": "Pyruvate dehydrogenase E1 component subunit beta" } }, "id": "APKECPOBEN_850", "locus": "GCA002090215_00828" }, { "type": "cds", "sequence": "contig_1", "start": 470426, "stop": 470662, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGAGTGACCCTGTGAAGCGGTTACGTAACTTCTTGTTTGACCGTGGTATTCTAACCCCACAACAAGAAGAGGAAATGGTAGCTAAGATTGAACAAGAAGTGCAAGCTGCATATGAGGTAATGGTTAGCAAAACGCCAGTTACCTTGGATGAAGTGTTTGATTATAACTATGAAAAGTTGACTCCTGATCTAGCGCGCCAAAAGGCTGAAGCTAAAAAATACTTTAAAGACTAA", "aa": "MKSDPVKRLRNFLFDRGILTPQQEEEMVAKIEQEVQAAYEVMVSKTPVTLDEVFDYNYEKLTPDLARQKAEAKKYFKD", "aa_hexdigest": "22a018bb5e0a188eee3497f3ddf07ced", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9202.413899999992, "isoelectric_point": 5.180442237854003 }, "id": "APKECPOBEN_851", "locus": "GCA002090215_00829" }, { "type": "cds", "sequence": "contig_1", "start": 470951, "stop": 471061, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00676.26" ], "nt": "ATGTTGCACTACAAGAAACTCCCTAACGTAGCCGTTACGATGATTGGGGATGGTGGTACAGCTGAAGGTGAGTTTTATGAAGCGATGAACATTGCTTCCATTCACAAGTGA", "aa": "MLHYKKLPNVAVTMIGDGGTAEGEFYEAMNIASIHK", "aa_hexdigest": "cd628000bc1b3f624d4cf663fe100233", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00676.26", "name": "E1_dh", "length": 34, "aa_cov": 0.9444444444444444, "hmm_cov": 1.0, "evalue": 1.8658217411139447e-09, "score": 32.36134338378906, "start": 3, "stop": 36 } ], "seq_stats": { "molecular_weight": 3937.5207, "isoelectric_point": 5.9887495040893555 }, "id": "APKECPOBEN_852", "locus": "GCA002090215_00830" }, { "type": "cds", "sequence": "contig_1", "start": 471164, "stop": 471316, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00676.26" ], "nt": "ATGTTGAACTTTGCGCCTAACTTAGGTGAGGAAGCACTGCAAGTTGGTATGGGCATGGGTTTAAATGAAAATGACTGGTTCTGCCCTACCTTCCGTTCCGGTGCGTTAATGTTGTACCGTGGTGTTAAACCAGAACAGCTTTTACTTTACTGA", "aa": "MLNFAPNLGEEALQVGMGMGLNENDWFCPTFRSGALMLYRGVKPEQLLLY", "aa_hexdigest": "fdbb75e2166b67bf059591c6d23abef4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00676.26", "name": "E1_dh", "length": 45, "aa_cov": 0.9, "hmm_cov": 1.0, "evalue": 7.352504588570254e-08, "score": 27.122459411621094, "start": 5, "stop": 49 } ], "seq_stats": { "molecular_weight": 5636.5244999999995, "isoelectric_point": 4.584314918518066 }, "id": "APKECPOBEN_853", "locus": "GCA002090215_00831" }, { "type": "cds", "sequence": "contig_1", "start": 471332, "stop": 471502, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAATTTTGATTAAAAACAAAGTCCCTACTACACTTTACCAAGTGTATGACAATGAGGGTAAGTTAATGGACCCTAATCACAAGATTACACTTAGCAACGAACAGTTAAAGCACGCTTTTTACCTCATGAATCTTTCCCGAATCATGGACAAGAAGATGCTTGTTTGA", "aa": "MAILIKNKVPTTLYQVYDNEGKLMDPNHKITLSNEQLKHAFYLMNLSRIMDKKMLV", "aa_hexdigest": "5bab2ac35b6accac1ac55ef5124ba67c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6594.8087, "isoelectric_point": 9.398539543151855 }, "id": "APKECPOBEN_854", "locus": "GCA002090215_00832" }, { "type": "cds", "sequence": "contig_1", "start": 471523, "stop": 471651, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGATCAGTGAACTGGGTTTAGTAGACGTTTACTTTCTTCCCCACTACAATAAGCCGTTCAACTTTGTGTTAGCAGCCGTGTTGCAAGCGCTTGGTTTTAGTTACTATACCCCTAAAAATAAATAA", "aa": "MLISELGLVDVYFLPHYNKPFNFVLAAVLQALGFSYYTPKNK", "aa_hexdigest": "445622aaa8dfa37c456841e475e603d1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4822.619100000001, "isoelectric_point": 8.085060691833494 }, "id": "APKECPOBEN_855", "locus": "GCA002090215_00833" }, { "type": "cds", "sequence": "contig_1", "start": 471706, "stop": 472371, "strand": "-", "frame": 1, "gene": "nox", "product": "NADH oxidase", "db_xrefs": [ "COG:COG0446", "COG:I", "EC:1.6.3.4", "GO:0008137", "SO:0001217", "UniRef:UniRef50_Q49408", "UniRef:UniRef90_P75389" ], "nt": "ATGTTGGACAAACCAGCTGGCAATAACTTTGATGAAGAGTTTACCAACGAGTTGGAAAAAGCCATGAAGAAAGCGGGCATTAACCTAATGATGGGTAGTGCCGTTAAGGGCTTTATTGTTGATGCGGACAAAAATGTCGTTAAAGGTGTTGAAACCGATAAGGGTCGGGTGGATGCTGACCTTGTCATCCAATCGATTGGGTTCCGCCCAAACACCCAGTTTGTCCCTAAAGACAGGCAGTTTGAGTTTAACCGTAACGGTTCGATTAAGGTTAATGAATACCTCCAAGCCTTAAATCATGAAAATGTGTATGTTATTGGTGGGGCTGCTGCCATTTATGATGCAGCTAGTGAACAATACGAAAACATTGACTTAGCTACCAACGCTGTGAAAAGTGGTTTAGTCGCTGCTATGCACATGATTGGTAGCAAGGCTGTAAAGTTAGAGTCCATTGTTGGTACCAATGCGCTGCACGTATTTGGTTTAAATCTAGCAGCTACTGGTTTAACCGAAAAACGTGCCAAAATGAACGGTTTTGATGTGGGAGTGAGCATTGTTGATGACAACGACCGTCCCGAATTTATGGGGACCTTTGACAAGGTGCGCTTTAAGTTAATTTACGACAAGAAGACGCTCCGTTTGTTGGGTGCCCAATTACTTTCGTGA", "aa": "MLDKPAGNNFDEEFTNELEKAMKKAGINLMMGSAVKGFIVDADKNVVKGVETDKGRVDADLVIQSIGFRPNTQFVPKDRQFEFNRNGSIKVNEYLQALNHENVYVIGGAAAIYDAASEQYENIDLATNAVKSGLVAAMHMIGSKAVKLESIVGTNALHVFGLNLAATGLTEKRAKMNGFDVGVSIVDDNDRPEFMGTFDKVRFKLIYDKKTLRLLGAQLLS", "aa_hexdigest": "3f23ecc26d2f5074f2ab9cc1410e1184", "start_type": "ATG", "rbs_motif": null, "genes": [ "nox" ], "truncated": "both", "pseudogene": { "start": 471523, "stop": 472671, "observations": { "mutation": [ 471708, 472404, 472620 ], "stop": [ 471708, 472404, 472620 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75389", "query_cov": 1.0, "subject_cov": 0.4613778705636743, "identity": 1.0, "score": 429.0, "evalue": 7.4e-148, "gene_start": 1, "gene_end": 221, "reference_start": 198, "reference_end": 418, "reference_sequence": "MKKVIVIGVNHAGTSFIRTLLSKSKDFQVNAYDRNTNISFLGCGIALAVSGVVKNTEDLFYSTPEELKAMGANVFMAHDVVGLDLDKKQVIVKDLATGKETVDHYDQLVVASGAWPICMNVENEVTHTQLQFNHTDKYCGNIKNLISCKLYQHALTLIDSFRHDKSIKSVAIVGSGYIGLELAEAAWQCGKQVTVIDMLDKPAGNNFDEEFTNELEKAMKKAGINLMMGSAVKGFIVDADKNVVKGVETDKGRVDADLVIQSIGFRPNTQFVPKDRQFEFNRNGSIKVNEYLQALNHENVYVIGGAAAIYDAASEQYENIDLATNAVKSGLVAAMHMIGSKAVKLESIVGTNALHVFGLNLAATGLTEKRAKMNGFDVGVSIVDDNDRPEFMGTFDKVRFKLIYDKKTLRLLGAQLLSWNTNHSEIIFYIALAVQKKMLISELGLVDVYFLPHYNKPFNFVLAAVLQALGFSYYTPKNK" }, "paralog": false, "identity": 0.9921465968586387, "score": 746.0, "evalue": 3.92e-275, "description": "Internal stop codon at 471708, 472404, 472620. Nonsense mutation around 471708, 472404, 472620.", "psc": { "uniref90_id": "UniRef90_P75389", "gene": "nox", "product": "NADH oxidase", "ec_ids": [ "1.6.3.4" ], "uniref50_id": "UniRef50_Q49408", "cog_id": "COG0446", "cog_category": "I", "go_ids": [ "GO:0008137" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75389", "EC:1.6.3.4", "UniRef:UniRef50_Q49408", "COG:COG0446", "COG:I", "GO:0008137" ] }, "pscc": { "uniref50_id": "UniRef50_Q49408", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49408" ], "product": "NADH oxidase" } }, "id": "APKECPOBEN_856", "locus": "GCA002090215_00834" }, { "type": "cds", "sequence": "contig_1", "start": 472402, "stop": 472608, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGTGGAAAACGAAGTAACCCACACCCAATTGCAATTCAACCACACTGATAAGTACTGTGGCAATATTAAGAACTTAATTAGTTGTAAGTTGTACCAACACGCCCTTACCTTAATTGACAGCTTCCGTCATGACAAGTCAATTAAATCAGTAGCCATTGTGGGCTCGGGTTACATTGGTTTAGAGTTAGCAGAAGCGGCTTGA", "aa": "MNVENEVTHTQLQFNHTDKYCGNIKNLISCKLYQHALTLIDSFRHDKSIKSVAIVGSGYIGLELAEAA", "aa_hexdigest": "2dc24f838a546b751a95cb4b1fe21983", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7592.557099999999, "isoelectric_point": 6.380313301086425 }, "id": "APKECPOBEN_857", "locus": "GCA002090215_00835" }, { "type": "cds", "sequence": "contig_1", "start": 472618, "stop": 472962, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF07992.20" ], "nt": "ATGAAGAAAGTGATTGTTATCGGTGTCAATCACGCCGGCACCAGTTTTATCAGAACCCTTTTAAGCAAGAGCAAAGACTTTCAGGTCAACGCTTATGATCGCAATACCAACATCTCCTTTTTGGGTTGTGGAATTGCCTTGGCAGTTAGTGGTGTAGTTAAAAACACCGAAGACCTGTTCTACTCCACCCCTGAAGAGCTAAAAGCTATGGGTGCTAATGTCTTTATGGCTCATGATGTAGTTGGTCTTGATCTAGACAAAAAACAAGTAATCGTGAAGGACTTGGCCACTGGTAAGGAAACTGTGGACCACTATGATCAATTGGTAGTAGCCTCTGGAGCTTGA", "aa": "MKKVIVIGVNHAGTSFIRTLLSKSKDFQVNAYDRNTNISFLGCGIALAVSGVVKNTEDLFYSTPEELKAMGANVFMAHDVVGLDLDKKQVIVKDLATGKETVDHYDQLVVASGA", "aa_hexdigest": "5b3d93c88d0a16c4b7e8d15b26fa8f75", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07992.20", "name": "Pyr_redox_2", "length": 115, "aa_cov": 0.9912280701754386, "hmm_cov": 0.9478260869565217, "evalue": 2.9448643165599065e-16, "score": 55.137691497802734, "start": 2, "stop": 114 } ], "seq_stats": { "molecular_weight": 12303.003799999991, "isoelectric_point": 6.256517982482911 }, "id": "APKECPOBEN_858", "locus": "GCA002090215_00836" }, { "type": "cds", "sequence": "contig_1", "start": 473030, "stop": 473563, "strand": "-", "frame": 3, "gene": "apt", "product": "adenine phosphoribosyltransferase", "db_xrefs": [ "COG:COG0503", "COG:F", "EC:2.4.2.7", "GO:0002055", "GO:0003999", "GO:0005737", "GO:0006166", "GO:0006168", "GO:0016208", "GO:0044209", "KEGG:K00759", "RefSeq:WP_010874751.1", "SO:0001217", "UniParc:UPI0000125CDF", "UniRef:UniRef100_P75388", "UniRef:UniRef50_P75388", "UniRef:UniRef90_P75388" ], "nt": "GTGAAACAGAAACTCCAAGCGCTTGATCGAGCTATTAAGCGATTTAATGACTTTCCCACTCCGGGAATCCTCTTTTACGACATTACGCCAATTTTTTTAAACAGTGAATTGTTTGAGTTTGTACTGGAACAAATGGCACAGTTTATCCAGGAAGTTAAAGCTGATGGCATCGTATGTCCCGAAGCGCGTGGTTTTATCTTTGGTGGTGCTTTGGCTTCCAAAACCAAACTACCCCTAGTGTTAGTGCGCAAACCCCATAAGCTATCAGGTGAGTTAGCGCGTGAAACCTATGATTTAGAATACCGCCAAAACTCAATCTTGGAAATGCGGGTTGATGCACTAGAAAACTGCAAGCGCTGTGTGATTGTTGATGACTTACTAGCTACTGCTGGTACGGTTGCAGCGATTGACAAACTAATAGCGCGTTTAGGTAGTCAAACTGTGGGTTATTGTTTTTTAATTGAACTGCAAAAACTGCACGGTAAAGCAAAGCTACAACCAAATGTGGCAACCAAGATCTTGCTCCACTATTAA", "aa": "MKQKLQALDRAIKRFNDFPTPGILFYDITPIFLNSELFEFVLEQMAQFIQEVKADGIVCPEARGFIFGGALASKTKLPLVLVRKPHKLSGELARETYDLEYRQNSILEMRVDALENCKRCVIVDDLLATAGTVAAIDKLIARLGSQTVGYCFLIELQKLHGKAKLQPNVATKILLHY", "aa_hexdigest": "5e39454662b44fa81a07024d889a5b2c", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000125CDF", "ncbi_nrp_id": "WP_010874751.1", "uniref100_id": "UniRef100_P75388", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000125CDF", "RefSeq:WP_010874751.1", "UniRef:UniRef100_P75388" ] }, "ips": { "uniref100_id": "UniRef100_P75388", "uniref90_id": "UniRef90_P75388" }, "psc": { "uniref90_id": "UniRef90_P75388", "gene": "apt", "product": "adenine phosphoribosyltransferase", "ec_ids": [ "2.4.2.7" ], "uniref50_id": "UniRef50_P75388", "cog_id": "COG0503", "cog_category": "F", "kegg_orthology_id": "K00759", "go_ids": [ "GO:0002055", "GO:0003999", "GO:0005737", "GO:0006166", "GO:0006168", "GO:0016208", "GO:0044209" ] }, "pscc": { "uniref50_id": "UniRef50_P75388", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75388" ], "product": "Adenine phosphoribosyltransferase" }, "genes": [ "apt" ], "id": "APKECPOBEN_859", "locus": "GCA002090215_00837" }, { "type": "cds", "sequence": "contig_1", "start": 473598, "stop": 473849, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTACGCTGCCTTTAACTTTGGTGGTATTAACGGCACGATTAACCTAGTGATCTTCTACTTAATTGCGATTGTGGCCTTGTTTAGCGTGTTTGTCAATGGCTTACCGCTGTTTAGCTTTGGTTTAATCAATGGTTGGTTAAGTCCGTACAACTATGTCCAAATCCATATTACCTTACGCCACAAAAAGCAAATGTTTAAGGAAGTGGATCAAATTGAAGAACAGTTGATTAGTGGAATTAACAGCTTTTAA", "aa": "MYAAFNFGGINGTINLVIFYLIAIVALFSVFVNGLPLFSFGLINGWLSPYNYVQIHITLRHKKQMFKEVDQIEEQLISGINSF", "aa_hexdigest": "fc43ab3fc82cab266e16351a1a6b4022", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9445.953199999996, "isoelectric_point": 6.702078819274903 }, "id": "APKECPOBEN_860", "locus": "GCA002090215_00838" }, { "type": "cds", "sequence": "contig_1", "start": 473895, "stop": 474446, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGACCAAAACATCGAGTAACTACTCGATCTTAACGGAAAACAACGACATCCAAACGGACTTCTTTATCAGTAAGAACCAGCACGACTTCTTTGGTAAGAAAAAGTGGAGCTTGCTGTTTATTTGGTTGACAGTTCTCGCCTTTGGGGTGGTGATGCTCGGCCTTTATCTGGGCGCACCACAACTGTTAGGTAACTTTTTAGCCGCTGACATCGAATCGAGTACTGGCATTATGTTAGGTGTGGGGGTGATTAGCTTGCTCTATCTCTTGTATTCGCTACCGCGCTATGGTGTCGCTTATGGGATTAGCTACCTAATTGCTTTAATTCTGTTAGCCGCTGGACTTTTTGGGGCAATGTACCTCGCTAACTTTATCTTTAGGATTGACCAGTCCACAATTCAACTAATTATCTTTGTGTACCTGTTCTGGCTGTTCTTCCAAGCCCGCATTGGTCAAACTACCATTTGGACTTACTGTTACTGGTTTAAACGCAGCTTGAAGGACCGGGTCTTTGTGAAAAACCTCTTTAACGACAATGTTAACAGCCAATGA", "aa": "MTKTSSNYSILTENNDIQTDFFISKNQHDFFGKKKWSLLFIWLTVLAFGVVMLGLYLGAPQLLGNFLAADIESSTGIMLGVGVISLLYLLYSLPRYGVAYGISYLIALILLAAGLFGAMYLANFIFRIDQSTIQLIIFVYLFWLFFQARIGQTTIWTYCYWFKRSLKDRVFVKNLFNDNVNSQ", "aa_hexdigest": "76a70d4e574f6633854a977e17852675", "start_type": "TTG", "rbs_motif": "AAAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 20991.397200000003, "isoelectric_point": 9.161811637878419 }, "id": "APKECPOBEN_861", "locus": "GCA002090215_00839" }, { "type": "cds", "sequence": "contig_1", "start": 474480, "stop": 474662, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATAGAAACCCACATTTGCTGGTTATTGAGTGCCTTAGTCGTGATTTACCTCTCCAACTACCAGGTACAACAGTTAGGTACCTTAATGGCAGTTTCAGCGATTACCTCTTACTTCTTAAACTATGGTTTAGGAGTAATCTTAGTGTGGTTGTTTGTGTCATCCCATTCCGGGTTAGAATGA", "aa": "MIETHICWLLSALVVIYLSNYQVQQLGTLMAVSAITSYFLNYGLGVILVWLFVSSHSGLE", "aa_hexdigest": "ac302e42dfe3dbde8c9e7e7eca00c1a4", "start_type": "ATG", "rbs_motif": "AAAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6706.840099999998, "isoelectric_point": 5.364145851135253 }, "id": "APKECPOBEN_862", "locus": "GCA002090215_00840" }, { "type": "cds", "sequence": "contig_1", "start": 474693, "stop": 475622, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000AF44FC6", "UniRef:UniRef90_UPI000AF44FC6" ], "nt": "GTGCGCAACATCTTTAACGTTCCTAAAGGTGGTACTGATTACCTCACCTTTAACGAACGTGAAAAGAACCTCTGGGACTTCTTGCACGCTGAGGGTAACTTTTCCTCTGGTGATAATTTGTTTTTGCAAAAAAATAATAATGGTAATTCACCAATTACCAAAGCTAGTGACATTACCTTAAAAAATATCTATTACATCTATGCAGCACCGACACATTTCTCACAAGTTGCAACCAAAGACAATAACAATAAGGACGCTGCTGATGTTGTCGCTGTAGCTAACAGCGCGGACACTAGAAAACTGCGCAGCGGTGATGCTACTGGTTTTTCTGGTTTATTCTCCAATTACATCTTGGCAGAAATTAGAACAGAAGATCCGGTAGCTAAGAATGGTGATCCGGTTAAGGTAAACGCCACTTTACAAAGCTTTTTGGATAGCAATAACCAACGCTTAGAACACGGTGGTAACATTAAGTTCCAAGTAGCTAACTTTGTTAACAATGATGGTTCATACGTTCCTGCTTCTTACTTGGAAGCTAGTAGGGTAAAGGTGCTGTTGAGCAATGGTTTTCCCGAAACCGCTACCATTGCAGCTGCTAACACCAAGTTAGTTAATGCACCGCTTTCCAACACTTTAGCACGCGATGTTTCTAACTTTGCTTCGAGCTTCATTGCCTTGGGTGTAATTATCCTGCTCGCTACAATTGCGTTAGGTATTCGTTACAAGCTACTTGGCCTTTACAAAGCCTTAGCCTTAGCGCTTAGTGCCATCTCTTCGCTCGCCTTCTTTAGTGCCGTTGGTGGTGTCGTTGATGTCTTTTCGTTTGTCGGCATCTTCTTTGTGGTTGCCGTTAACATTATTAACCTGATCACTCTGGCGGAACTGTTTTTGCGTAACATTAAGAACAACGCCTCAATTGTGGAATCGTGA", "aa": "MRNIFNVPKGGTDYLTFNEREKNLWDFLHAEGNFSSGDNLFLQKNNNGNSPITKASDITLKNIYYIYAAPTHFSQVATKDNNNKDAADVVAVANSADTRKLRSGDATGFSGLFSNYILAEIRTEDPVAKNGDPVKVNATLQSFLDSNNQRLEHGGNIKFQVANFVNNDGSYVPASYLEASRVKVLLSNGFPETATIAAANTKLVNAPLSNTLARDVSNFASSFIALGVIILLATIALGIRYKLLGLYKALALALSAISSLAFFSAVGGVVDVFSFVGIFFVVAVNIINLITLAELFLRNIKNNASIVES", "aa_hexdigest": "e9974aded01bb0a08c4857adc34fad6c", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 474390, "stop": 475793, "observations": { "mutation": [ 474482, 474695, 475709 ], "stop": [ 474482, 474695, 475709 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000AF44FC6", "query_cov": 1.0, "subject_cov": 0.42270861833105333, "identity": 0.99, "score": 572.0, "evalue": 3.81e-199, "gene_start": 1, "gene_end": 309, "reference_start": 58, "reference_end": 366, "reference_sequence": "MDFIKSKSLTDSDYNTGNGQKSVEGSGKWFKDKANGSAQNGNKTLVLWKDKEGAVQYVRNIFNVPKGGTDYLTFNEREKNLWDFLHAEGNFSSGDNLFLQKNNNGNSPITKASDITLKNIYYIYAAPTHFSQVATKDNNNKDAADVVTVANSADTRKLRSGDATGFSGLFSNYILAEIRTEDPVAKNGDPVKVNATLQSFLDSNNQRLEHGGNIKFQVANFVNNDGSYVPASYLEASRVKVLLSNGFPETATIAAANTKLVNAPLSNTLARDVSNFASSFIALGVIILLATIALGIRYKLLGLYKALALALSAISSLAFFNAVGGVVDVFSFVGIFFVVAVNIINLITLAELFLRNIKNNASIVESWKLCLKRSFFTMIETHICWLLSALVVIYLSNYQVQQLGTLMAVSAITSYFLNYGLGVILVWLFVSSHSGLEWRFFLYKKDAQALTKTSSNYSILTENNDIQTDFFISKNQHDFFGKKKWSLLFIWLTVLAFGVVMLGLYLGAPQLLGNFLAADIESSTGIMLGVGVISLLYLLYSLPRYGVAYGISYLIALILLAAGLFGAMYLANFIFRIDQSTIQLIIFVYLFWLFFQARIGQTTIWTYCYWFKRSLKDRVFVKNLFNDNVNSQWRLDLIGSSVLILLFIVYAAFNFGGINGTINLVIFYLIAIVALFSVFVNGLPLFSFGLINGWLSPYNYVQIHITLRHKKQMFKEVDQIEEQLISGINSF" }, "paralog": false, "identity": 0.987152034261242, "score": 896.0, "evalue": 0.0, "description": "Internal stop codon at 474482, 474695, 475709. Nonsense mutation around 474482, 474695, 475709.", "psc": { "uniref90_id": "UniRef90_UPI000AF44FC6", "uniref50_id": "UniRef50_UPI000AF44FC6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000AF44FC6", "UniRef:UniRef50_UPI000AF44FC6" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000AF44FC6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000AF44FC6" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 33346.40520000004, "isoelectric_point": 7.841628074645997 }, "id": "APKECPOBEN_863", "locus": "GCA002090215_00841" }, { "type": "cds", "sequence": "contig_1", "start": 476106, "stop": 476513, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGGACTGAAAAAGCGGGTTTCCTTTGACTGGCTCTTAAAGATTGGAATTATCCTGGTCTTAGCTTTTTTGGGCCTGTTTGGAATTATTTTTGGTAGTTATAAGCTTCTAAACGACAGTCGCTTAGGGGTAGTGTTCAACGGTTCCACTACCACCACTGTTTATTTTTTAAACCATAAGTCCAAGGACGCTAACAAGGAGTTAGATCCTACCCAGAAAAAGGACGGCAATGGCAGCGGTAACAATACTGAAATGATTACCGATGTGAACGGTTTTCTCAACAATATTGAAAAGAGTTACGCCAACAGCCTCTACGCCCAGGGTTTTTCGTCTGTTAACATCACGAAAAACTCACACGATACAAGTGCAAAAGAACTTTCAGATAAAGGAGTTTTTGACAGTTCTTGA", "aa": "MGLKKRVSFDWLLKIGIILVLAFLGLFGIIFGSYKLLNDSRLGVVFNGSTTTTVYFLNHKSKDANKELDPTQKKDGNGSGNNTEMITDVNGFLNNIEKSYANSLYAQGFSSVNITKNSHDTSAKELSDKGVFDSS", "aa_hexdigest": "6216c419a5b9c1d13101f2e5658d69cf", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14794.53789999998, "isoelectric_point": 8.803883171081544 }, "id": "APKECPOBEN_864", "locus": "GCA002090215_00842" }, { "type": "cds", "sequence": "contig_1", "start": 476556, "stop": 476666, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCCACTTTAGTTGAAATTGAACGCGATTTTTTACAAAAAACAGCCCAAAAATTTGCACCTGAAGTGGTTGCGTTGATTACTAAAGCACTCGATTACTCCAAAAAGTGA", "aa": "MATLVEIERDFLQKTAQKFAPEVVALITKALDYSKK", "aa_hexdigest": "ddd94cdd00635392bc3c5ea8e08e66f6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4096.786800000001, "isoelectric_point": 8.099243736267091 }, "id": "APKECPOBEN_865", "locus": "GCA002090215_00843" }, { "type": "cds", "sequence": "contig_1", "start": 476748, "stop": 478211, "strand": "+", "frame": 3, "gene": "spoT", "product": "putative guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase", "db_xrefs": [ "COG:COG0317", "COG:TK", "EC:3.1.7.2", "GO:0005886", "GO:0008893", "GO:0015970", "SO:0001217", "UniRef:UniRef50_P75386", "UniRef:UniRef90_P75386" ], "nt": "ATGGACAGCAACACCGTGTGTGCTGGTCTGTTACACGACATTATTGAAGATACCCAGGTCACTGAAGCTGATTTAACTGCCATTTTTGGTAAAGAAATTACTGATTTAGTGGTAAAAGTTACCAAAATTACCAGTGAATCCAAAAAACAACGCCAGCTAAACCGAAAGAAAGAAGATTTAAACCTCAAAAGTTTGGTTAACATTGCGATGAGCTCGCAGCAAGAGGTCAATGCTTTGGTGTTAAAGTTGGCCGACCGACTCGACAACATCTCCTCAATTGAATTTTTAGCAGTCGAAAAACAAAAGATTATTGCTAAAGAAACACTCGAACTGTATGCCAAGATAGCCGGTCGAATTGGAATGTATCCCGTTAAAACTCAACTAGCCGATCTTTCCTTTAAGGTATTAGATCCAAAAAACTTTAACAATACGCTGTCCAAGATTAACCAGCAAAAGGTCTTTTATGACAACGAATGGGGCAACTTTAAAAAACAGTTGGAAGAGATGCTGGAACAAAACCAGATTGAATACCGCTTAGAAAGTCGAATTAAAGGCATTTATTCTACTTATCAAAAATTAACCTTTCACGAGCAGAACATTGCCAAAATCCACGATCTGTTCGCCATTCGTTTAATTGTGAAGTCAGAGTTGGATTGTTACCACTTACTTGGTTTAATCCACCTCAACTTTACCGTGTTAATGAAACACTTTAAGGATTACATAGCATCCCCCAAACAAAACTTTTACCAATCAATCCACACGACTGTGCGCTTGAAGGGTTTAAATGTGGAAATCCAGATTAGAACCCAACGAATGGATCATGTGAGCAAGTACGGTTTTGCTAGCCACTGGATTTACAAAGAGAAAAAGGAAGGGTTATTGGCAAGTGCTTTACAAGTAAATTACTTAAACAGTAAACAAATGCATTCGCGCGATTTTTTTAAACGAATCTTTGGTACTGATATTATTAAGGTTAATGTAAGTTCCGATAACGAACCTAACATAGTCAAAAAACTCAATGTTGAAAGTAACAGCAAGTTGCTCGATATTGCTTATGAGTTGTATCCCAAACAGTTCAATAAATTAGAAAAAATCAAACTCGATGGGGTTGAGGTTATGTCGTTTGACGTAACTGCCGAAAACGAAATGGTAATTGAGTTTTGTTTTGGTAAAACCAATAACCTCAAACGGCGTTGGTTACGTTATATGAATAACCCCGTATTTCGCGAACGGGTTAAAAAGGATTTAAACAAGTTAAAGAAAGCGGTTAAGTACAGTGAACTACCTCTATACGAAAAGGCCTTGGAAGAGTTGCACTTAAAGCTAGCTGATGAAACCCAGATTAAACAACGCTTAAATGCTTTAGGGATCAAAAAACTGACAGAGTTCTTGGAATTAATCGAATACCCCCACTTCCCTAAAAATGAGCATTTGTACTTTTTAGCTAGTAACAATCAGAAGTGA", "aa": "MDSNTVCAGLLHDIIEDTQVTEADLTAIFGKEITDLVVKVTKITSESKKQRQLNRKKEDLNLKSLVNIAMSSQQEVNALVLKLADRLDNISSIEFLAVEKQKIIAKETLELYAKIAGRIGMYPVKTQLADLSFKVLDPKNFNNTLSKINQQKVFYDNEWGNFKKQLEEMLEQNQIEYRLESRIKGIYSTYQKLTFHEQNIAKIHDLFAIRLIVKSELDCYHLLGLIHLNFTVLMKHFKDYIASPKQNFYQSIHTTVRLKGLNVEIQIRTQRMDHVSKYGFASHWIYKEKKEGLLASALQVNYLNSKQMHSRDFFKRIFGTDIIKVNVSSDNEPNIVKKLNVESNSKLLDIAYELYPKQFNKLEKIKLDGVEVMSFDVTAENEMVIEFCFGKTNNLKRRWLRYMNNPVFRERVKKDLNKLKKAVKYSELPLYEKALEELHLKLADETQIKQRLNALGIKKLTEFLELIEYPHFPKNEHLYFLASNNQK", "aa_hexdigest": "f5bb08320ed47dbb1182a72114c6396a", "start_type": "ATG", "rbs_motif": null, "genes": [ "spoT" ], "truncated": "both", "pseudogene": { "start": 476517, "stop": 478514, "observations": { "mutation": [ 476529, 476664, 476742, 478209, 478449 ], "stop": [ 476529, 476664, 476742, 478209, 478449 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75386", "query_cov": 1.0, "subject_cov": 0.6643929058663028, "identity": 0.998, "score": 937.0, "evalue": 0.0, "gene_start": 1, "gene_end": 487, "reference_start": 78, "reference_end": 564, "reference_sequence": "MFYNWLKLYKFSKMATLVEIERDFLQKTAQKFAPEVVALITKALDYSKKWHGEQKRLSGEPFFIHPLRTALRLVEWNMDSNTVCAGLLHDIIEDTQVTEADLTAIFGKEITDLVVKVTKITSESKKQRQLNRKKEDLNLKSLVNIAMSSQQEVNALVLKLADRLDNISSIEFLAVEKQKIIAKETLELYAKIAGRIGMYPVKTQLADLSFKVLDPKNFNNTLSKINQQKVFYDNEWGNFKKQLEEMLEQNQIEYRLESRIKGIYSTYQKLTFHEQNIAKIHDLFAIRLIVKSELDCYHLLGLIHLNFTVLMKHFKDYIASPKQNFYQSIHTTVRLKGLNVEIQIRTQRMDHVSKYGFASHWIYKEKKEGLLASALQVNYLNSKQMHSRDFFKRIFGTDIIKVNVSSDNEPNIVKKLNVESNSKLLDIAYELYPKQFNKLEKIKLDGVEVMSFDVTAENEMVIEFCFGKTNNLKRRWLRYMNNHVFRERVKKDLNKLKKAVKYSELPLYEKALEELHLKLADETQIKQRLNALGIKKLTEFLELIEYPHFPKNEHLYFLASNNQKWRELIKPIKFALSQAVFQNSYFEQIEGIYITKIVIETCCTKIPDMPEQVIGILMKNILRVHLHDCRELANQKQPKIIPLYWNAHQLKMRPRKFRCQINIRGVWSETTVNKIVQTIIEGDSYLERIIPKIDKQKDEFELNITMFIDNYHQLITIMEQITTKNISYVWKYL" }, "paralog": false, "identity": 0.9909774436090225, "score": 1292.0, "evalue": 0.0, "description": "Internal stop codon at 476529, 476664, 476742, 478209, 478449. Nonsense mutation around 476529, 476664, 476742, 478209, 478449.", "psc": { "uniref90_id": "UniRef90_P75386", "gene": "spoT", "product": "Probable guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase", "ec_ids": [ "3.1.7.2" ], "uniref50_id": "UniRef50_P75386", "cog_id": "COG0317", "cog_category": "TK", "go_ids": [ "GO:0005886", "GO:0008893", "GO:0015970" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75386", "EC:3.1.7.2", "UniRef:UniRef50_P75386", "COG:COG0317", "COG:TK", "GO:0005886", "GO:0008893", "GO:0015970" ] }, "pscc": { "uniref50_id": "UniRef50_P75386", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75386" ], "product": "Probable guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase" } }, "id": "APKECPOBEN_866", "locus": "GCA002090215_00844" }, { "type": "cds", "sequence": "contig_1", "start": 478218, "stop": 478451, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTAAACCCATTAAGTTCGCTTTATCACAAGCTGTGTTCCAAAACTCTTACTTTGAACAAATTGAGGGAATTTACATCACCAAAATTGTGATTGAAACCTGTTGCACAAAGATTCCTGACATGCCAGAACAAGTTATTGGCATCTTAATGAAAAACATTCTCAGGGTACACCTTCATGATTGTAGGGAATTAGCTAATCAAAAGCAACCCAAAATTATTCCACTTTATTGA", "aa": "MIKPIKFALSQAVFQNSYFEQIEGIYITKIVIETCCTKIPDMPEQVIGILMKNILRVHLHDCRELANQKQPKIIPLY", "aa_hexdigest": "694f63dd98f8263921d95be02114cff8", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8959.653899999994, "isoelectric_point": 8.504556465148926 }, "id": "APKECPOBEN_867", "locus": "GCA002090215_00845" }, { "type": "cds", "sequence": "contig_1", "start": 478470, "stop": 478706, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCGACCACGTAAATTCCGCTGTCAAATTAACATTCGCGGTGTATGGAGTGAAACAACGGTCAATAAAATTGTTCAAACCATTATTGAAGGTGATAGCTATTTAGAACGAATTATTCCCAAGATCGATAAACAAAAAGATGAGTTTGAGCTCAACATTACTATGTTTATCGACAACTATCACCAACTAATCACAATTATGGAGCAGATTACTACTAAGAATATTAGCTACGTTTGA", "aa": "MRPRKFRCQINIRGVWSETTVNKIVQTIIEGDSYLERIIPKIDKQKDEFELNITMFIDNYHQLITIMEQITTKNISYV", "aa_hexdigest": "ed0906eba75f04ab6eb63e460804548d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9362.786699999993, "isoelectric_point": 7.868124580383301 }, "id": "APKECPOBEN_868", "locus": "GCA002090215_00846" }, { "type": "cds", "sequence": "contig_1", "start": 478715, "stop": 479371, "strand": "-", "frame": 3, "gene": null, "product": "putative MG279-like protein", "db_xrefs": [ "GO:0005886", "RefSeq:WP_010874754.1", "SO:0001217", "UniParc:UPI000013956D", "UniRef:UniRef100_P75385", "UniRef:UniRef50_P47521", "UniRef:UniRef90_P75385" ], "nt": "ATGTTTAAGTTTTTAAAAAAGCTTTCCACTTTCCTAATTGTTTTGATTGGTATTCTACTTGTTGGTGGCATTACAGCAGCGGGTTACTTTGCCTTTGAAAACCGTGAACCTATTAATAACTATTACAAGGAAGGTTATAACAAGGTTAAACAATATAACGAGGAAATTAAAAAAGTTTCAAAGTCACTTTCTAGTAACGAACTTGTAAAAACGTTAGGTGATGTTGAAAGTTCAATTAAAGAAGGAAAAGAATTGACAAAATTACTGGGTGACAGTGCTTTAGAAAGCAGTTTTAACCAACTCGAAGATTCATTAAGCAAGGTTAACAACTTCTCAAAAGGATCTACTTTTACTGAAGTTAAGAACACAATTGAAAAGATTAATCAATACGTGGACGAAATCTTAAAACGTTTTCCAAATCCTAACGAAAATGATCAGTTCAAGGAATACGTAACTAATATTTCACAAATTGTCTTTTATGTCGGGGTATCTATTATTGGTACATTTGTTGTTTCTGGTGCCTTATTGTTTATTTTCACCAAACGCGTTTATGGGGTACGAGTATCGCGCTTTAACCCACAAAGGCTATTGAAGAAACACTTAGTGTTACTTTTACAAAAGAACCAAGATGTTTATGACGAAGTATTTGAAAGTTAA", "aa": "MFKFLKKLSTFLIVLIGILLVGGITAAGYFAFENREPINNYYKEGYNKVKQYNEEIKKVSKSLSSNELVKTLGDVESSIKEGKELTKLLGDSALESSFNQLEDSLSKVNNFSKGSTFTEVKNTIEKINQYVDEILKRFPNPNENDQFKEYVTNISQIVFYVGVSIIGTFVVSGALLFIFTKRVYGVRVSRFNPQRLLKKHLVLLLQKNQDVYDEVFES", "aa_hexdigest": "d09e9188827edeafeb0adb5eb5c30297", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013956D", "ncbi_nrp_id": "WP_010874754.1", "uniref100_id": "UniRef100_P75385", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013956D", "RefSeq:WP_010874754.1", "UniRef:UniRef100_P75385" ] }, "ips": { "uniref100_id": "UniRef100_P75385", "uniref90_id": "UniRef90_P75385" }, "psc": { "uniref90_id": "UniRef90_P75385", "product": "Uncharacterized protein MG279 homolog", "uniref50_id": "UniRef50_P47521", "go_ids": [ "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47521", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47521" ], "product": "Uncharacterized protein MG279" }, "genes": [], "id": "APKECPOBEN_869", "locus": "GCA002090215_00847" }, { "type": "cds", "sequence": "contig_1", "start": 479371, "stop": 480234, "strand": "-", "frame": 1, "gene": null, "product": "putative MG280-like protein", "db_xrefs": [ "GO:0005886", "RefSeq:WP_010874755.1", "SO:0001217", "UniParc:UPI0000139575", "UniRef:UniRef100_P75384", "UniRef:UniRef50_P75384", "UniRef:UniRef90_P75384" ], "nt": "ATGCTCCTTTTTATTAATCGCTTTGCCAAAACAATCATTCTGCTCTTCGGGATGCTTGTCTTTTTGGTTTTGTTAGGTCTTGGCGGTGCTGCACTTTACTTTAAAGACAACGCAGCTAAACTTTACATTGATACCCGCAAATCGATCGACAGTTCCTTTGACTCTTCTCAAGCGTTTATAGATACTTATAATGGAAGTTCCAGTAAGTTTAGTGTTGAATCCATTAATAAACAAATAGAAGAGGTTAAGAAGAAAGTTGAAGAATCAACTAAAAAGTTAGAGGAGTACGAAAAGCAAATAAACCAAGCTAAAGGCTTAAACGGCTATCTTGTGAGTCCGGAAAAACTAAAGGAGTTACAAGAAGCTAAGAAGAGTCTACAAGCTACTAAGAGTCAAATAGAAAAGTATGCAAATACTTTAAAAACCGCTAATAATGGTAAAACTGGTCAAAATGGCACATCTTCTTCGACTATTCCAATTACTAAGATTTCTGGTTCAACAATTAGTGTAAGTACCAGAGATACTAACGGTAAAACCAATAGTGCTTTGAAAGATATTCAAGAGTTCTCTACACAAGCAAATGACATCATTAAACAGTACAAAGAAATTAAAAACAAAATTCCAACAGAAAAACAGTTTAATGAATATTACACCATAGGCGCAATTACCTTAGTTAGTGTTTCTGGTGGCGTTTTAGCAGTGCTAATAGTCTCTACTGTAATGACCTTCTTAGGTTCGAAAAAGTTAGGACTTAGAACGTTTTCGCGTTTAACCAGTACTGATCAAATTGCTGATCATGTTAATGACATCTTAGATCGTTATCCAGAGTTGGAAGATGCTGTTTTAGAGGAGCTTGATCAATAA", "aa": "MLLFINRFAKTIILLFGMLVFLVLLGLGGAALYFKDNAAKLYIDTRKSIDSSFDSSQAFIDTYNGSSSKFSVESINKQIEEVKKKVEESTKKLEEYEKQINQAKGLNGYLVSPEKLKELQEAKKSLQATKSQIEKYANTLKTANNGKTGQNGTSSSTIPITKISGSTISVSTRDTNGKTNSALKDIQEFSTQANDIIKQYKEIKNKIPTEKQFNEYYTIGAITLVSVSGGVLAVLIVSTVMTFLGSKKLGLRTFSRLTSTDQIADHVNDILDRYPELEDAVLEELDQ", "aa_hexdigest": "f1b7061bd5b38c2f1dc8d895aff3344d", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000139575", "ncbi_nrp_id": "WP_010874755.1", "uniref100_id": "UniRef100_P75384", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139575", "RefSeq:WP_010874755.1", "UniRef:UniRef100_P75384" ] }, "ips": { "uniref100_id": "UniRef100_P75384", "uniref90_id": "UniRef90_P75384" }, "psc": { "uniref90_id": "UniRef90_P75384", "product": "Uncharacterized protein MG280 homolog", "uniref50_id": "UniRef50_P75384", "go_ids": [ "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75384", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75384" ], "product": "Uncharacterized protein MG280 homolog" }, "genes": [], "id": "APKECPOBEN_870", "locus": "GCA002090215_00848" }, { "type": "cds", "sequence": "contig_1", "start": 480296, "stop": 480802, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF22806.1", "PFAM:PF20757.2", "PFAM:PF22805.1" ], "nt": "GTGAAAAACACTAATGAAAAAAATGTCAATAAAGACAACGACACCGTTTACGCTGCTCTAAAAGACATTAATCTCCACTTAGAGGAAACTTACACTCATGGCGGCAACACGATGTACCGGGTGAACGAAAATTACTATCCGGGTGCCAGTGCTTACGAAGCGGAACGCGCTACCCGTGACAGTGAGTTCCAAAAGGAAATTGTACAACGCGCTGAACTTATTGGGGTTGTCTTTGAATATGGAGTGAAAAACCTCCGTCCGGGTTTAAAGTACACCGTGAAGTTTGAAAGTCCACAAGAACAAGTGGCATTAAAGAGTACCGATAAGTTCCAACCAGTTATTGGTAGTGTCACTGATATGAGCAAATCAGTTACTGATTTAATCGGTGTATTACGTGATAACGCCGAAATTCTCAATATTACTAATGTTTCTAAAGATGAAACCGTTGTAGCGGAATTGAAAGAAAAATTGGATCGGGAAAATGTTTTCCAAGAGATTCGAACTTAA", "aa": "MKNTNEKNVNKDNDTVYAALKDINLHLEETYTHGGNTMYRVNENYYPGASAYEAERATRDSEFQKEIVQRAELIGVVFEYGVKNLRPGLKYTVKFESPQEQVALKSTDKFQPVIGSVTDMSKSVTDLIGVLRDNAEILNITNVSKDETVVAELKEKLDRENVFQEIRT", "aa_hexdigest": "0390aac5cde1d52b2939ce76a7f62944", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF22806.1", "name": "M_C", "length": 99, "aa_cov": 0.5833333333333334, "hmm_cov": 1.0, "evalue": 9.687675098274515e-54, "score": 175.40232849121094, "start": 71, "stop": 168 }, { "id": "PF20757.2", "name": "M_large_dom", "length": 42, "aa_cov": 0.25, "hmm_cov": 1.0, "evalue": 3.60097558951543e-19, "score": 64.40087890625, "start": 1, "stop": 42 }, { "id": "PF22805.1", "name": "M_smaller_dom", "length": 28, "aa_cov": 0.16666666666666666, "hmm_cov": 1.0, "evalue": 1.2241443237250765e-22, "score": 74.97817993164062, "start": 43, "stop": 70 } ], "seq_stats": { "molecular_weight": 19127.170200000015, "isoelectric_point": 5.087453651428221 }, "id": "APKECPOBEN_871", "locus": "GCA002090215_00849" }, { "type": "cds", "sequence": "contig_1", "start": 480830, "stop": 481672, "strand": "-", "frame": 3, "gene": null, "product": "IgG-blocking protein M", "db_xrefs": [ "GO:0005515", "GO:0016020", "SO:0001217", "UniRef:UniRef50_P47523", "UniRef:UniRef90_P75383" ], "nt": "GTGCCTAAGGTAGAGGTGAGCGGTTTGATTAAAACCAGTTCTACCTTTATCACGCCAAACTTTAAAGCGGGTTACTACGACCAAGTAGCAGCGGACGGGAAAACGCTCAAATATTACCAATCAACCGAATACTTTAACAACCGGGTAGTAATGCCAATTTTGCAAACTACCAACGGCACTTTAACTGCTAACAATAGGGCTTACGATGATATCTTTGTCGATCAAGGTGTCCCTAAGTTCCCTGGTTGGTTCCATGACGTGGATAAGGCTTACTATGCTGGCTCCAATGGACAAAGCGAGTACTTGTTTAAAGAGTGGAATTACTATGTTGCTAACGGTTCACCGTTATACAACGTTTACCCAAACCATCACTTTAAGCAAATAAAAACGATTGCCTTTGATGCTCCAAGGATTAAGCAAGGTAATACTGACGGCATTAACCTCAATTTAAAACAACGTAATCCTGATTACGTCATCATTAATGGTTTAACTGGTGATGGTTCTACCTTAAAGGATTTGGAATTACCAGAAAGCGTTAAAAAGGTGTCAATTTATGGTGACTACCACTCGATTAATGTTGCTAAGCAAATTTTTAAAAACGTGTTAGAGTTGGAGTTTTACTCTACTAACCAAGACAATAACTTTGGTTTTAATCCACTAGTTTTAGGCGATCACACTAATATTATCTATGACCTTTTTGCTTCCAAGCCGTTTAATTACATTGATTTAACTAGTTTAGAACTTAAAGATAACCAAGACAATATTGATGCATCTAAGTTAAAGCGTGCTGTTAGTGACATTTACATTCGTCGTCGTTTTGAACGACAAATGCAAGGTTACTGA", "aa": "MPKVEVSGLIKTSSTFITPNFKAGYYDQVAADGKTLKYYQSTEYFNNRVVMPILQTTNGTLTANNRAYDDIFVDQGVPKFPGWFHDVDKAYYAGSNGQSEYLFKEWNYYVANGSPLYNVYPNHHFKQIKTIAFDAPRIKQGNTDGINLNLKQRNPDYVIINGLTGDGSTLKDLELPESVKKVSIYGDYHSINVAKQIFKNVLELEFYSTNQDNNFGFNPLVLGDHTNIIYDLFASKPFNYIDLTSLELKDNQDNIDASKLKRAVSDIYIRRRFERQMQGY", "aa_hexdigest": "94e1cc0758c17af4793dcbe08767d015", "start_type": "GTG", "rbs_motif": "AAAA", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 480527, "stop": 481972, "observations": { "mutation": [ 480832 ], "stop": [ 480832 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75383", "query_cov": 1.0, "subject_cov": 0.48109965635738833, "identity": 0.996, "score": 570.0, "evalue": 6.89e-201, "gene_start": 1, "gene_end": 280, "reference_start": 125, "reference_end": 404, "reference_sequence": "MKLNFKIKDKKTLKRLKKGGFWALGLFGAAINAFSAVLIVNEVLRLQSGETLIASGRSGNLSFQLYSKVNQNAKSKLNSISLTDGGYRSEIDLGDGSNFREDFRNFANNLSEAITDAPKDLLRPVPKVEVSGLIKTSSTFITPNFKAGYYDQVAADGKTLKYYQSTEYFNNRVVMPILQTTNGTLTANNRAYDDIFVDQGVPKFPGWFHDVDKAYYAGSNGQSEYLFKEWNYYVANGSPLYNVYPNHHFKQIKTIAFDAPRIKQGNTDGINLNLKQRNPDYVIINGLTGDGSTLKDLELPESVKKVSIYGDYHSINVAKQIFKNVLELEFYSTNQDNNFGFNPLVLGDHTNIIYDLFASKPFNYIDLTSLELKDNQDNIDASKLKRAVSDIYIRRRFERQMQGYWAGGYIDRYLVKNTNEKNVNKDNDTVYAALKDINLHLEETYTHGGNTMYRVNENYYPGASAYEAERATRDSEFQKEIVQRAELIGVVFEYGVKNLRPGLKYTVKFESPQEQVALKSTDKFQPVIGSVTDMSKSVTDLIGVLRDNAEILNITNVSKDETVVAELKEKLDRENVFQEIRT" }, "paralog": false, "identity": 0.997920997920998, "score": 968.0, "evalue": 0.0, "description": "Internal stop codon at 480832. Nonsense mutation around 480832.", "psc": { "uniref90_id": "UniRef90_P75383", "product": "IgG-blocking protein M", "uniref50_id": "UniRef50_P47523", "go_ids": [ "GO:0005515", "GO:0016020" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75383", "UniRef:UniRef50_P47523", "GO:0005515", "GO:0016020" ] }, "pscc": { "uniref50_id": "UniRef50_P47523", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47523" ], "product": "Uncharacterized protein MG281" } }, "id": "APKECPOBEN_872", "locus": "GCA002090215_00850" }, { "type": "tRNA", "sequence": "contig_1", "start": 482332, "stop": 482405, "strand": "-", "gene": "trnG", "product": "tRNA-Gly(tcc)", "amino_acid": "Gly", "anti_codon": "tcc", "score": 69.3, "nt": "GCGAGTATAGTTTAGTGGTAGAACATCAGTCTTCCAAGCTGATCGTGTCGGTTCGATTCCGATTACTCGCTCCA", "db_xrefs": [ "SO:0000260" ], "anti_codon_pos": [ 482371, 482373 ], "id": "APKECPOBEN_31", "locus": "GCA002090215_00851" }, { "type": "tRNA", "sequence": "contig_1", "start": 482412, "stop": 482497, "strand": "-", "gene": "trnL", "product": "tRNA-Leu(taa)", "amino_acid": "Leu", "anti_codon": "taa", "score": 70.5, "nt": "GCCCAAGTGGCGGAATGGTAGACGCATGGGATTTAAGATCCCACGCTAGCAATAGCGTGCCGGTTCAAGTCCGGCTTTGGGCACCA", "db_xrefs": [ "SO:0000264" ], "anti_codon_pos": [ 482462, 482464 ], "id": "APKECPOBEN_30", "locus": "GCA002090215_00852" }, { "type": "tRNA", "sequence": "contig_1", "start": 482498, "stop": 482572, "strand": "-", "gene": "trnK", "product": "tRNA-Lys(ttt)", "amino_acid": "Lys", "anti_codon": "ttt", "score": 77.0, "nt": "GACTCACTAGCTCAGCGGTAGAGCATTTGACTTTTAATCAAAGGGTCCCGAGTTCGATCCTCGGGTGAGTCACCA", "db_xrefs": [ "SO:0000265" ], "anti_codon_pos": [ 482545, 482547 ], "id": "APKECPOBEN_29", "locus": "GCA002090215_00853" }, { "type": "tRNA", "sequence": "contig_1", "start": 482616, "stop": 482690, "strand": "-", "gene": "trnQ", "product": "tRNA-Gln(ttg)", "amino_acid": "Gln", "anti_codon": "ttg", "score": 70.6, "nt": "TGGGATGTAGCCAAGCGGTAAGGCAATAGACTTTGACTCTATCATGCGATGGTTCGATCCCATCCATCCCAGCCA", "db_xrefs": [ "SO:0000261" ], "anti_codon_pos": [ 482656, 482658 ], "id": "APKECPOBEN_28", "locus": "GCA002090215_00854" }, { "type": "tRNA", "sequence": "contig_1", "start": 482696, "stop": 482782, "strand": "-", "gene": "trnY", "product": "tRNA-Tyr(gta)", "amino_acid": "Tyr", "anti_codon": "gta", "score": 72.9, "nt": "GGACAGGTAGCGAAGTGGCTAAACGCTTCTGACTGTAGATCAGACACCTTCATGGTTTCGGGAGTTCGAATCTCTCCCTGTCCACCA", "db_xrefs": [ "SO:0000272" ], "anti_codon_pos": [ 482774, 482776 ], "id": "APKECPOBEN_27", "locus": "GCA002090215_00855" }, { "type": "cds", "sequence": "contig_1", "start": 482815, "stop": 483294, "strand": "-", "frame": 1, "gene": "greA", "product": "transcription elongation factor GreA", "db_xrefs": [ "COG:COG0782", "COG:K", "GO:0003677", "GO:0006354", "GO:0032784", "GO:0070063", "SO:0001217", "UniRef:UniRef50_P47524", "UniRef:UniRef90_P78019" ], "nt": "ATGGAACTTAACAAAAATTACCTTACCGAAGAGGGGCTAAAACAATTAGAAGCGGAGCTGGAACACCTCATTCAAGTAAAACGTCCCGCTATTATTAAGTTATTGCAAGAAGCCCGTGATCAGGGTGACTTGAGTGAAAACGCTGATTACGATGCAGCAAAAGCACAGCAGGGTGAAATTGAAACCCGCATTGCTGAAATCCAAGACATTTTAGCCAACGTTAAGTTAATTAATGAGTCGCAAACAAAAAAGGCTAATAAGGTTACCCTGGGTAGTACGGTCGAAATTTACGATTACAGTTCGAAAACACACGAAAAGTACACCATTGTGGGAGCTTTAGAGGCTAATCCCGAAGAACACCGCATCTCCAATGAATCCCCTTTGGCACACGCCATTTATGGTCGTTTAGTGGATGATGAATGTGACGTAGTTGGCATAGAAGTGCCCTACCGGGTCAAAATTGTTAAGATCATTAACTGA", "aa": "MELNKNYLTEEGLKQLEAELEHLIQVKRPAIIKLLQEARDQGDLSENADYDAAKAQQGEIETRIAEIQDILANVKLINESQTKKANKVTLGSTVEIYDYSSKTHEKYTIVGALEANPEEHRISNESPLAHAIYGRLVDDECDVVGIEVPYRVKIVKIIN", "aa_hexdigest": "68a4b0aa89c6f5b05cd466a26ebf32f6", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P78019", "query_cov": 1.0, "subject_cov": 0.99375, "identity": 1.0, "score": 301.0, "evalue": 1.76e-103, "valid": true, "gene": "greA", "product": "transcription elongation factor GreA", "uniref50_id": "UniRef50_P47524", "cog_id": "COG0782", "cog_category": "K", "go_ids": [ "GO:0003677", "GO:0006354", "GO:0032784", "GO:0070063" ] }, "pscc": { "uniref50_id": "UniRef50_P47524", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47524" ], "product": "Transcription elongation factor GreA" }, "genes": [ "greA" ], "id": "APKECPOBEN_873", "locus": "GCA002090215_00856" }, { "type": "cds", "sequence": "contig_1", "start": 483425, "stop": 483565, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_014574968.1", "SO:0001217", "UniParc:UPI0001DD63D5", "UniRef:UniRef100_A0AB38W775", "UniRef:UniRef50_A0AB38W775", "UniRef:UniRef90_A0AB38W775" ], "nt": "ATGACAACGAAAGACAATTCTGGCATTAAAAGTAAAAGACGGATTCGTTTTTTAGCTTTAGGAATTATTATTTTGATTCTTGTAATTGCTGTAATCACCATTTTATTTGTCACGGGTGTAATTACCCGCGCTAACCAATAA", "aa": "MTTKDNSGIKSKRRIRFLALGIIILILVIAVITILFVTGVITRANQ", "aa_hexdigest": "86e8640e47c62ed2fa104c8b81b0bc6b", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD63D5", "ncbi_nrp_id": "WP_014574968.1", "uniref100_id": "UniRef100_A0AB38W775", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD63D5", "RefSeq:WP_014574968.1", "UniRef:UniRef100_A0AB38W775" ] }, "ips": { "uniref100_id": "UniRef100_A0AB38W775", "uniref90_id": "UniRef90_A0AB38W775" }, "psc": { "uniref90_id": "UniRef90_A0AB38W775", "uniref50_id": "UniRef50_A0AB38W775" }, "pscc": { "uniref50_id": "UniRef50_A0AB38W775", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W775" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5082.190099999999, "isoelectric_point": 11.999967765808105 }, "id": "APKECPOBEN_874", "locus": "GCA002090215_00857" }, { "type": "cds", "sequence": "contig_1", "start": 483801, "stop": 484025, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGAGCAAGTTCAACTACCGACCCTTTCCTTTTACAGTGAGTACCAAAAGGAAAAGCGTCATGTGGAGGGCTTTAATCCTGAGCTTTTTTTAATAGAGCAAATAGGTACTAAAAAACTCCATGATCCGCTAGTACTACGTCCTACTAGTGAAATTGCTTTTTGTAACTTATGGAAAAAACAGAGCTTTTCTTACCAAAACCTTCCCGTTATCTATAACCAGTGA", "aa": "MEQVQLPTLSFYSEYQKEKRHVEGFNPELFLIEQIGTKKLHDPLVLRPTSEIAFCNLWKKQSFSYQNLPVIYNQ", "aa_hexdigest": "3447f991c38ca5aaebaa72b61424f513", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8832.037399999997, "isoelectric_point": 6.714697074890137 }, "id": "APKECPOBEN_875", "locus": "GCA002090215_00858" }, { "type": "cds", "sequence": "contig_1", "start": 484266, "stop": 484463, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCCGATGGGCAGGCATTGCAGTGTGCCACGAGCCATGATTTAGGGCAAACCTTTACCAAACCGTTTGGGCTAACCTTTCAAAACCAAGCCAACCAACAGGCCATTCCCTACAGCTTTAGTTGTGGAATTTCTACAAGAATCCTCGGTGCTTTACTGTTAACCCACAGTGATGACTTTGGACTGGTACTACCATGA", "aa": "MPDGQALQCATSHDLGQTFTKPFGLTFQNQANQQAIPYSFSCGISTRILGALLLTHSDDFGLVLP", "aa_hexdigest": "58d5c3affe15ccc5b236590c3e57401c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6983.8252999999995, "isoelectric_point": 5.18828601837158 }, "id": "APKECPOBEN_876", "locus": "GCA002090215_00859" }, { "type": "cds", "sequence": "contig_1", "start": 484542, "stop": 485027, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF09180.16" ], "nt": "GTGCAAACGTTGTTGGAACAATTAGCAATCCGTTTTCAATTTATTAAGGTCGAAAACCAGCTCGGTAAACAGTTAGGACAGGGTGAAGTCAACGGCATTCCCTTTCAACTAATAGTTGACAATCCCCAAACAGTCAACATCTTTAACCGGTTAACCAGGGTCAAAACAGCTTACAGTTTCGAACAGTTGGCAAGCCGATTTGTGGAGCTCGTGCAACAGTACCATCAAGCAATGTATGACAAAGCCAAAGCAGTGGTACAGCAAAAGGTGGTGCAAGCCACTACTTTAAAGCAAATTGAACAAGCCTTTAATGACAAAAAGGCTGTTTTGTGTGCGGTTCGTTTAACCGATACATTAGAACAGCAATTAAAGGAACGGTACCAGGTCACGGTGCGCTGTTGCTTAGAGCAGCTTCAAAAGCCCCAAATTTGTCCGTTTAGCGGTGAGTCAGCACAGGACTATGTCCTGATTGCGCGTGCCTATTAA", "aa": "MQTLLEQLAIRFQFIKVENQLGKQLGQGEVNGIPFQLIVDNPQTVNIFNRLTRVKTAYSFEQLASRFVELVQQYHQAMYDKAKAVVQQKVVQATTLKQIEQAFNDKKAVLCAVRLTDTLEQQLKERYQVTVRCCLEQLQKPQICPFSGESAQDYVLIARAY", "aa_hexdigest": "46cf1f9a1daf19c4c12de05acbaa0777", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF09180.16", "name": "ProRS-C_1", "length": 70, "aa_cov": 0.40372670807453415, "hmm_cov": 1.0, "evalue": 3.114382756992783e-11, "score": 38.782405853271484, "start": 97, "stop": 161 } ], "seq_stats": { "molecular_weight": 18543.232399999994, "isoelectric_point": 8.897168922424317 }, "id": "APKECPOBEN_877", "locus": "GCA002090215_00860" }, { "type": "cds", "sequence": "contig_1", "start": 485172, "stop": 485540, "strand": "+", "frame": 3, "gene": null, "product": "putative MG284-like protein", "db_xrefs": [ "RefSeq:WP_010874759.1", "SO:0001217", "UniParc:UPI0000139587", "UniRef:UniRef100_P75381", "UniRef:UniRef50_P47526", "UniRef:UniRef90_P75381" ], "nt": "ATGAGTTTTAAAGTTAAAAACCAGAGTAAGCACCTATACTCTTTAATGACGAAATTTAAACGTTCGCAACTGATCTTGAAGCACCAAAGCAATAACTTTGCCTCAGAGTTGTGGAACGAAGAGGACATTATCCGCTCCAAGCAGTTTATCGAACTTATAGAAGACACGCTGCTCCACCTCAAGAAGGATACGGTCGACTTTATTTACGACATCTTCATTTATGGGAAGAAGCCGTGTGATATTAGTTACTCCAATTCGACCTATTACAAAAAGTTAAACAAGGCTGCAAATAGTTTTTTTGACCATTTTGTCTGGGAGGCTCCAATTTATAAGACAAAGGAGCTTAAAAATGACAATTCTCACAGTTAG", "aa": "MSFKVKNQSKHLYSLMTKFKRSQLILKHQSNNFASELWNEEDIIRSKQFIELIEDTLLHLKKDTVDFIYDIFIYGKKPCDISYSNSTYYKKLNKAANSFFDHFVWEAPIYKTKELKNDNSHS", "aa_hexdigest": "3bb59ed69b6a3d1cb6f566a83d959a59", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000139587", "ncbi_nrp_id": "WP_010874759.1", "uniref100_id": "UniRef100_P75381", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139587", "RefSeq:WP_010874759.1", "UniRef:UniRef100_P75381" ] }, "ips": { "uniref100_id": "UniRef100_P75381", "uniref90_id": "UniRef90_P75381" }, "psc": { "uniref90_id": "UniRef90_P75381", "product": "Uncharacterized protein MG284 homolog", "uniref50_id": "UniRef50_P47526" }, "pscc": { "uniref50_id": "UniRef50_P47526", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47526" ], "product": "Uncharacterized protein MG284" }, "genes": [], "id": "APKECPOBEN_878", "locus": "GCA002090215_00861" }, { "type": "cds", "sequence": "contig_1", "start": 485521, "stop": 485751, "strand": "+", "frame": 1, "gene": null, "product": "DUF5443 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB33HMP7", "UniRef:UniRef90_A0AB33HMP7" ], "nt": "ATGACAATTCTCACAGTTAGACAAATTCAAAATAATTACTATACGGAATACTACATGGGTCCGGTTAGAAACTTTGCTGACAAAAAGGAAGTTTACTTTGATGCTAAGGTAAACAATATTGAAAGCAAAGTCAACAAGGATAAAGCCTTGTTACACATAACCCTGGCGTTAAAGTACGACAGTAACTTTCCCAGTAATATGTTCCAAGCTCAGTTTAAACTGGGTAATTGA", "aa": "MTILTVRQIQNNYYTEYYMGPVRNFADKKEVYFDAKVNNIESKVNKDKALLHITLALKYDSNFPSNMFQAQFKLGN", "aa_hexdigest": "3db421df654ed2b4b39175648bdcad95", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 485521, "stop": 485905, "observations": { "insertion": [ 485788 ], "mutation": [ 485749 ], "stop": [ 485749 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB33HMP7", "query_cov": 1.0, "subject_cov": 0.5984251968503937, "identity": 1.0, "score": 154.0, "evalue": 6.73e-47, "gene_start": 1, "gene_end": 76, "reference_start": 1, "reference_end": 76, "reference_sequence": "MTILTVRQIQNNYYTEYYMGPVRNFADKKEVYFDAKVNNIESKVNKDKALLHITLALKYDSNFPSNMFQAQFKLGNWSSEKIPLQKAPDKNPIWKIRFTIFMPLWKCHELPLLNGKLTALVSIFVKN" }, "paralog": false, "identity": 0.9921259842519685, "score": 253.0, "evalue": 2.88e-88, "description": "Internal stop codon at 485749. Frameshift due to insertion around 485788. Nonsense mutation around 485749.", "psc": { "uniref90_id": "UniRef90_A0AB33HMP7", "product": "Family of unknown function (DUF5443)", "uniref50_id": "UniRef50_A0AB33HMP7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB33HMP7", "UniRef:UniRef50_A0AB33HMP7" ] }, "pscc": { "uniref50_id": "UniRef50_A0AB33HMP7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB33HMP7" ] } }, "id": "APKECPOBEN_879", "locus": "GCA002090215_00862" }, { "type": "cds", "sequence": "contig_1", "start": 485839, "stop": 486099, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCCACGAACTGCCCTTATTAAACGGGAAATTAACCGCATTAGTGAGTATATTCGTGAAGAATTAACAATTGCTTTTCGCTTAAATGACAGTAGTGATCGCTACCATTGGAGTAACTATAACCTGTTCGATACAGTGGCAGCTGATATGTACCAAACGGTCATTAAAGAAACCGTTACCTTTGGTAACATGATTAGACTAAACCAACTCGAGGGCGAGAAGGAAGGTAACATCCAACAAAGTGAACTTAAATATGGTTGA", "aa": "MPRTALIKREINRISEYIREELTIAFRLNDSSDRYHWSNYNLFDTVAADMYQTVIKETVTFGNMIRLNQLEGEKEGNIQQSELKYG", "aa_hexdigest": "9a95947fe0086d9d3decd8c19b46e65b", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10178.338500000002, "isoelectric_point": 5.350277137756348 }, "id": "APKECPOBEN_880", "locus": "GCA002090215_00863" }, { "type": "cds", "sequence": "contig_1", "start": 486103, "stop": 486561, "strand": "+", "frame": 1, "gene": null, "product": "DUF5443 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W764", "UniRef:UniRef90_A0AB38W764" ], "nt": "ATGTTGGACTACCGCAATATGGGGCCGCTGGAAGAGGTTAATAATTTAATTAATATTAAGTTTAACCAACCAGTAAAAGTGGTAAGTGTTGGTGTGACCGTAAGCTATACAGCGCCTGATGGTAAACGCCAAGAGCTCAAAGATCAAGCTGAATACCAGCACACCCTCAACCCCAATGAAGAGTTCAAACTGAACTTTCCCAGACGGCTAAGCTTTAACAGGGTAACTAAAAAGTTGGACTTTGACCCCAATGGTAGTGCCGTGTTCCTACCACAGGGTGGCTTTGGTAGCTATGAGATCAAACTGGAGGCTAATGTCGGCAACCAGTTCTACACAATTGTGGCTACTAACAGCTTTGAGTACGCCCATCCGTTTGACGATCCCAAGACCAATGACTTCTTTCTGGTGCAGTACGCTCCAGTCTACAGTCTGTTTAACTTTAGCGATTTAATTCAGTAA", "aa": "MLDYRNMGPLEEVNNLINIKFNQPVKVVSVGVTVSYTAPDGKRQELKDQAEYQHTLNPNEEFKLNFPRRLSFNRVTKKLDFDPNGSAVFLPQGGFGSYEIKLEANVGNQFYTIVATNSFEYAHPFDDPKTNDFFLVQYAPVYSLFNFSDLIQ", "aa_hexdigest": "e0cfce36bda916afae4f31fcb9de000c", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 485839, "stop": 486561, "observations": { "mutation": [ 486097 ], "stop": [ 486097 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB38W764", "query_cov": 1.0, "subject_cov": 0.6333333333333333, "identity": 1.0, "score": 311.0, "evalue": 3.25e-106, "gene_start": 1, "gene_end": 152, "reference_start": 89, "reference_end": 240, "reference_sequence": "MPRTALIKREINRISEYIREELTIAFRLNDSSDRYHWSNYNLFDTVAADMYQTVIKETVTFGNMIRLNQLEGEKEGNIQQSELKYGWTMLDYRNMGPLEEVNNLINIKFNQPVKVVSVGVTVSYTAPDGKRQELKDQAEYQHTLNPNEEFKLNFPRRLSFNRVTKKLDFDPNGSAVFLPQGGFGSYEIKLEANVGNQFYTIVATNSFEYAHPFDDPKTNDFFLVQYAPVYSLFNFSDLIQ" }, "paralog": false, "identity": 0.9916666666666667, "score": 482.0, "evalue": 5.54e-176, "description": "Internal stop codon at 486097. Nonsense mutation around 486097.", "psc": { "uniref90_id": "UniRef90_A0AB38W764", "product": "Family of unknown function (DUF5443)", "uniref50_id": "UniRef50_A0AB38W764", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB38W764", "UniRef:UniRef50_A0AB38W764" ] }, "pscc": { "uniref50_id": "UniRef50_A0AB38W764", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W764" ] } }, "id": "APKECPOBEN_881", "locus": "GCA002090215_00864" }, { "type": "cds", "sequence": "contig_1", "start": 486568, "stop": 487161, "strand": "+", "frame": 1, "gene": null, "product": "putative MG286-like protein", "db_xrefs": [ "GO:0016020", "RefSeq:WP_010874761.1", "SO:0001217", "UniParc:UPI0000139592", "UniRef:UniRef100_P75379", "UniRef:UniRef50_P47528", "UniRef:UniRef90_P75379" ], "nt": "ATGATCTTCTCAATTAGTAAACGCAAGTTAATTTGTGGCTTTTCCCTAGTAGCCCTTACAATCGCTGGGATTGTTGGTGGGGTTTACTTAGTCACCAAAAACAATCAACAAACCACACCACAAACAAATCACTTTAATGTAGCTGACCCAGTCAACAAGGTCCCTAATTGGCGCAAACTAGGTCCAGAAACACAACGCGAACTGCGCGATCTTCTGTATCCCTTAGATGACACAGGTTACTTTATTTACAAGTACGGTGCCATTAGTCGCTACCTCCAAAGCCAAAAGGAACTGGATGAACTAGTCGATTACCGCACCGTGTTACCCTCCACCCAAAAGCACTTTAAGTATGATTCCTTTAACCAAAGCGTGTTGGAAAGTAAGCTGCGTAAGTGGTTAATGAAAGCCATTAAACAACATCCGTACTTTCAGCACTTTGAGTTTGATCCGGTGCTTAAAGCACAGTACAATATCAACATTCCTGCTCAAAAAATTACGGTGAATGCAGTTTGGTTTTACAAAAAGGATAATGATCTCACCACGGGCAAGCCAATCCGCTATTGGGATCAGTTTGAAATTAAACTAAAGCAGTAG", "aa": "MIFSISKRKLICGFSLVALTIAGIVGGVYLVTKNNQQTTPQTNHFNVADPVNKVPNWRKLGPETQRELRDLLYPLDDTGYFIYKYGAISRYLQSQKELDELVDYRTVLPSTQKHFKYDSFNQSVLESKLRKWLMKAIKQHPYFQHFEFDPVLKAQYNINIPAQKITVNAVWFYKKDNDLTTGKPIRYWDQFEIKLKQ", "aa_hexdigest": "a55d2ed0e9d28abbac6c23ab5e9e36e5", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000139592", "ncbi_nrp_id": "WP_010874761.1", "uniref100_id": "UniRef100_P75379", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139592", "RefSeq:WP_010874761.1", "UniRef:UniRef100_P75379" ] }, "ips": { "uniref100_id": "UniRef100_P75379", "uniref90_id": "UniRef90_P75379" }, "psc": { "uniref90_id": "UniRef90_P75379", "product": "Uncharacterized protein MG286 homolog", "uniref50_id": "UniRef50_P47528", "go_ids": [ "GO:0016020" ] }, "pscc": { "uniref50_id": "UniRef50_P47528", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47528" ], "product": "Uncharacterized protein MG286" }, "genes": [], "id": "APKECPOBEN_882", "locus": "GCA002090215_00865" }, { "type": "tRNA", "sequence": "contig_1", "start": 487441, "stop": 487515, "strand": "+", "gene": null, "product": "tRNA-Xxx", "score": 70.7, "nt": "AGGGGTATAGTTCAAAGGTAGAACATCTGTCTTCAAAATAGAGTGTTGTGGGTTCGAGTCCTGCTACCCCTGCCA", "db_xrefs": [], "id": "APKECPOBEN_12", "locus": "GCA002090215_00866" }, { "type": "cds", "sequence": "contig_1", "start": 487705, "stop": 487914, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGGCTATTTGAGTTTTGGTTATCTCAAAAAGCCAGCAACAGTGGTAGAGTTGCCCGAAGTAACGGTGGATGATCAAACCGGTGTGCTCGACATTAAGCAGTCTGTACCACCCAAACTAAAGGTTAACAAACAAGGCTTTGAACCTGTTTTCATCACCTTTACTAACACTAACAACAACGAGCAAGTTACCGTGGTTTTAAACAACTGA", "aa": "MGYLSFGYLKKPATVVELPEVTVDDQTGVLDIKQSVPPKLKVNKQGFEPVFITFTNTNNNEQVTVVLNN", "aa_hexdigest": "922f9de47a11d308e707933d08ee0b8d", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7668.682700000001, "isoelectric_point": 5.096036338806152 }, "id": "APKECPOBEN_883", "locus": "GCA002090215_00867" }, { "type": "cds", "sequence": "contig_1", "start": 487936, "stop": 488106, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTCTTACAGTTAGCTAAAGTGAGTGGGATTGATATTAATGAAAACAAACTGTTTAACACTGATTTTTTAAAGAAGTCGTTTTACTTTGCCGATAAATTTTCCCACCTTTCAGTGAACGGTAATGTGGAATACATGCGCAATATCTTAACAACTGTTGGGTTTGATTGA", "aa": "MFLQLAKVSGIDINENKLFNTDFLKKSFYFADKFSHLSVNGNVEYMRNILTTVGFD", "aa_hexdigest": "1c83aba5ab5176eff1197662a976d41e", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6482.352200000001, "isoelectric_point": 6.53480167388916 }, "id": "APKECPOBEN_884", "locus": "GCA002090215_00868" }, { "type": "cds", "sequence": "contig_1", "start": 488188, "stop": 488571, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13472.12" ], "nt": "TTGTTTATTGGTGATTCGATGACTCATGGTGTTGGTACTGACGGATACAGTAGTGCCCCACAACTGTTGGAACAAAGCTTGAAAAATGACTTTGGTCGTTACCGTGATGTAGTGATCAACAGTGCCATTAATGGTAGTAACACCTCCTTAGAGTTGTACATGCAAAATCATCGCTTTAAGCAGTACAAAAACATTGATGTTTATGTACTTAATTTGGGTACTAATGACATTAACCAGTTAGCGCAAGGGGTTTACACAGTCGAGCAATACAAACATAATCTTACCCAAATACTGGATCTGTTACATACGCAAAGTCCTCAAGCCCATGTTGTTTTAGCCAACATTGCTCCATCTAGCTTACTGCGAAGCTCTGAAAAGAACTGA", "aa": "MFIGDSMTHGVGTDGYSSAPQLLEQSLKNDFGRYRDVVINSAINGSNTSLELYMQNHRFKQYKNIDVYVLNLGTNDINQLAQGVYTVEQYKHNLTQILDLLHTQSPQAHVVLANIAPSSLLRSSEKN", "aa_hexdigest": "a985ec35f73bbc9cc5aa7107276c166a", "start_type": "TTG", "rbs_motif": "AAAA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13472.12", "name": "Lipase_GDSL_2", "length": 118, "aa_cov": 0.9133858267716536, "hmm_cov": 0.9491525423728814, "evalue": 6.049687752511509e-20, "score": 67.91608428955078, "start": 2, "stop": 117 } ], "seq_stats": { "molecular_weight": 14227.753399999985, "isoelectric_point": 6.158186531066893 }, "id": "APKECPOBEN_885", "locus": "GCA002090215_00869" }, { "type": "cds", "sequence": "contig_1", "start": 488926, "stop": 489240, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAGTGAAGACAAAGTTAGCTTAAGTTTGCAAGGAGAATTTGCGATTGGTAAACAGTTAGCACAAGTGTTAAAAGCTACTGGCGCTGAACCATTGCCAGTGGAAGTTACAGAACCATCACTAGAAGCTATACCAATTAATGAAGAAACCCAGTTTGATGTAAAAAATTACTACAGCTTTGGCCATTACCATGACACCAATGTCATTAATGACAGGATTGATTTTGATGATCCCAACTACAACTTAGAAACGGACTTTTTCAATAATGCTAAAGCTAATTTGGTAACTACTTCCAATCCCTTTACTGTTACTTGA", "aa": "MSEDKVSLSLQGEFAIGKQLAQVLKATGAEPLPVEVTEPSLEAIPINEETQFDVKNYYSFGHYHDTNVINDRIDFDDPNYNLETDFFNNAKANLVTTSNPFTVT", "aa_hexdigest": "c39648a9d1d8352e338100dbdf228006", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11644.65189999998, "isoelectric_point": 4.223273658752441 }, "id": "APKECPOBEN_886", "locus": "GCA002090215_00870" }, { "type": "cds", "sequence": "contig_1", "start": 489409, "stop": 489669, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTTGCTAGTGCTCGGTTTGAAGGTAAGATAGCAAACTTTGGTTTTGCCGGGCAGACTATGAAAGATATTAATGAGAGCTTTCAAGAACGGGTACTTTCAATTTACAAACCACGTACGCTAGTTTACCTCACTTCCCAAGCGGATGCAGAGCGGGTTGATTATTACGAGGAGTTTGTCAATAATCTAACCGCACTCATTAAAAAGCAAGTTGGTGAAAAGGCTGAAAAGGACAACTTTTTCATTTTGCAATTGCACTGA", "aa": "MFASARFEGKIANFGFAGQTMKDINESFQERVLSIYKPRTLVYLTSQADAERVDYYEEFVNNLTALIKKQVGEKAEKDNFFILQLH", "aa_hexdigest": "10e8c22a9cf686b7f2745657e6e1dd78", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9974.213899999993, "isoelectric_point": 5.775148582458496 }, "id": "APKECPOBEN_887", "locus": "GCA002090215_00871" }, { "type": "cds", "sequence": "contig_1", "start": 489865, "stop": 490101, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGCACTCAGAGTGATGTTTCACCACTCTTTGTGAAAAATATCAACATCCAACTACCGAGCTCCACCCATCAACAAAAGATTGAACCCTTAAATTTACCAACTAACATTAGCAAAACAGAAATAGCGAGTTGGGGTAGTGAATTCCAAAGCAAGGTATTGGACTTTTATGCTAGGGATGGTGAAGAACATGACAACAAAATTAGGTACCAACTCCCATCCTTAAGCTTTGCTTGA", "aa": "MGTQSDVSPLFVKNINIQLPSSTHQQKIEPLNLPTNISKTEIASWGSEFQSKVLDFYARDGEEHDNKIRYQLPSLSFA", "aa_hexdigest": "c33f8049d6badddb7a1fe65f6e5d6cd0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8836.776099999992, "isoelectric_point": 5.513689231872558 }, "id": "APKECPOBEN_888", "locus": "GCA002090215_00872" }, { "type": "cds", "sequence": "contig_1", "start": 490422, "stop": 491054, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTATTAAGAAGGGTTTTTTCGCGTTATCAAGTTGCACATTAGGTCTAGGTCTTATTTTAACTGCATGTGGTGCTAGGGGTAAATTTGACCAAGTTGACGATGGTGTTATTAAGCTAGCTACTTCAATCCAAAATAAGGACAGTATCGCTGCTTTAAACACCATTTACAAGAAGTATAAGGAACGCCATCCAGGGAGTTATCCAGTTCAAAATTTTCAGGTTCCCGGTGGTTACAGTGGTTTGCGGAACGACATTAGAACGCGTTTATCAGCTAAAGATGGCAACAACTTTTACAACATAGTACTCAACTATCCTGATGTTGTCTCCTCATTGGCTACAAGTGACATGCAACTAATATTGGATAACGTTGACACCAAGCTCCTTTCGAAAAATTTCTTGAGCTTTAACGAGCGAATTGGTGGTGTACGACAAAAGGGGATTTATGCAATTCCCATTTCCCTTTCCACTGAATTAATGGTGCTCAACGGACCGGTATTGCACTATATTTTGAACAGTGCTAAGAAGAAGGAAAACGGTGCTCAAATGATTAAAAGATCAGGTAGTTTTTCCACTGTTCAAAAAGGTACCATGGCAATCGACATGAATGATCAAAAGACCAAAGATCTTTGA", "aa": "MVIKKGFFALSSCTLGLGLILTACGARGKFDQVDDGVIKLATSIQNKDSIAALNTIYKKYKERHPGSYPVQNFQVPGGYSGLRNDIRTRLSAKDGNNFYNIVLNYPDVVSSLATSDMQLILDNVDTKLLSKNFLSFNERIGGVRQKGIYAIPISLSTELMVLNGPVLHYILNSAKKKENGAQMIKRSGSFSTVQKGTMAIDMNDQKTKDL", "aa_hexdigest": "fcd1555589bc2af8d9d9776fd99df763", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 23061.360500000003, "isoelectric_point": 9.57634334564209 }, "id": "APKECPOBEN_889", "locus": "GCA002090215_00873" }, { "type": "cds", "sequence": "contig_1", "start": 491586, "stop": 492368, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTACATTAATAAAGGTGGTGCTTATTCCAGTAACTTCTTAAAGTTTCACCAGTTAGCTTTTAGTGTGGGTTCCAGTTCTGGTTACCATTTTGCTTTTGCTGGTGAAAGCGCTAAACGCTTGGAATTTGGCCAGAAAGCAATCGAGTACCCACGTGACACTTACGAAATTAAGGCACCTACAAATAGCCAAAATGGTAATGGTACCCTTCTAGGTAGTTTTACTAAAAGTAAAAGTAATGGCAAAGAACAAAGTGGGCAAGATGAGGATAACCAGACCAGTGAAACGATTGAGCTTTACAAAAGCAGCGTTCCTAGTGGTAAAGAGGCGGGTAAAAATGCGCTTGCTATTACCAATCAACAACTAATTTCGGCTTTAGAAAATGCTGCAAAAGATAATAAAACCTCCCAACCACAAGCACGATCACTTACAGCTTCTGATCAAGTTCAAATTACACAAAGTTCAGATAAGGTTATTGGTTACATCACCACATCTAATTTGGACATTGACAACAACAATACCTTTGATGTTGGTAAGTTAAACGGTGATAAGTCAACCAGCAAAATCATTGTTAACGCTACTTTAAAAACCTTAAACAAGATCAACACCTTGCAAAGCGAAGAGGGCATTATTCTGCCCCATCCACAAAAATATAAGAGTACTGATCCACAAGCTGTCGCTACCGTTCAGGGTCCAAGTATTATTGGTGTTCATGCTAACGCTAAGGAAAACGCTGAAACCCAAAAATTTATCAACTGGTTTATTAACCAAAAAGAAACTTGA", "aa": "MYINKGGAYSSNFLKFHQLAFSVGSSSGYHFAFAGESAKRLEFGQKAIEYPRDTYEIKAPTNSQNGNGTLLGSFTKSKSNGKEQSGQDEDNQTSETIELYKSSVPSGKEAGKNALAITNQQLISALENAAKDNKTSQPQARSLTASDQVQITQSSDKVIGYITTSNLDIDNNNTFDVGKLNGDKSTSKIIVNATLKTLNKINTLQSEEGIILPHPQKYKSTDPQAVATVQGPSIIGVHANAKENAETQKFINWFINQKET", "aa_hexdigest": "0835509447fd7f3e56e0325ebb624ea6", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 28234.853800000015, "isoelectric_point": 7.804881095886231 }, "id": "APKECPOBEN_890", "locus": "GCA002090215_00874" }, { "type": "cds", "sequence": "contig_1", "start": 492411, "stop": 492704, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACCGCAGCACAATACTTTGCTAAGAGCTCTGGTTATGTTTTACCTTACAGTGAAACGTTTACTAAACAAAGTGAAGACGAACACAGCACAACTAAAGACGCTTATAAGATCTTGAAAGATGTAAACGATGGTAAGTTAGTTGGTTACAGTGATCCAAGTGATTTCCGTTCTGGTAAATTCCGTGACACAATTGTCGCTGCTTTTAGTGGTGCTGTAAGCTCTAAAGCTGACTTTAATAAGTTCTTTAAAGGTTTTGAACAACAATTAGGTCAAGAATACAGAAGGGGTTAA", "aa": "MTAAQYFAKSSGYVLPYSETFTKQSEDEHSTTKDAYKILKDVNDGKLVGYSDPSDFRSGKFRDTIVAAFSGAVSSKADFNKFFKGFEQQLGQEYRRG", "aa_hexdigest": "a8becba91ff954c1fb3d18cd369c6064", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10889.878499999993, "isoelectric_point": 7.900423240661622 }, "id": "APKECPOBEN_891", "locus": "GCA002090215_00875" }, { "type": "cds", "sequence": "contig_1", "start": 492678, "stop": 493094, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W7Y3" ], "nt": "GTGGTTTTCGTCACTTTGTCCGTTCTTATCATTCCTTCACCCCGAGCCGGTTCAGAGCAGGAGGTTGGCTTGGTCGAGGGTGGGTCATTTGGAGCCGCCTGTAGTCGTCGTCTGACTGCCACCAAGCGTGGCTTGGACGTTGGCTTTGAGGCCAACGCCGACATTCGAGAACGTCGGGAGAAAGGAACGCGAACCACTTGTGTTCGTACTAGTTCCGCTGGACGACCCCGCGCCGGTTTTAGGCGCGGTTATATCATCCACGCCCCGGTGGTTCTTCCCATACCTGTACCCATTCCCAATCCAGGGCACGTAATCCAACGCCTGGTCAAGGAGTGCCCCCGCGAGGTTGAACCCGGTGGAGAACCGGGAAAGGGTGGGGGAGAAGTGGGATTAACCCCTTCTGTATTCTTGACCTAA", "aa": "MVFVTLSVLIIPSPRAGSEQEVGLVEGGSFGAACSRRLTATKRGLDVGFEANADIRERREKGTRTTCVRTSSAGRPRAGFRRGYIIHAPVVLPIPVPIPNPGHVIQRLVKECPREVEPGGEPGKGGGEVGLTPSVFLT", "aa_hexdigest": "c168e273047fd4cef6fee8150d1325e2", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0027E159D2", "query_cov": 0.9710144927536232, "subject_cov": 0.950354609929078, "identity": 0.7759999999999999, "score": 194.0, "evalue": 9.34e-62, "valid": false, "uniref50_id": "UniRef50_A0AB38W7Y3" }, "pscc": { "uniref50_id": "UniRef50_A0AB38W7Y3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W7Y3" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14625.698999999984, "isoelectric_point": 9.90139293670654 }, "id": "APKECPOBEN_892", "locus": "GCA002090215_00876" }, { "type": "cds", "sequence": "contig_1", "start": 493695, "stop": 493835, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAGCTTCCAGCTCGGCGGGTGGCTGGTCACCTTCACCGACTTTGTCAAACCCCGGTCGGGGTACCTCGGCTTGCAGTTAACGGGCTTGGATGTGAGTGAAGCGACGCAACGGGAGTTAATTTGGGCCCCCCGGCCCTGA", "aa": "MSFQLGGWLVTFTDFVKPRSGYLGLQLTGLDVSEATQRELIWAPRP", "aa_hexdigest": "d1a807325feb97f81d59371f14f26a5a", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5182.904700000001, "isoelectric_point": 5.971697807312012 }, "id": "APKECPOBEN_893", "locus": "GCA002090215_00877" }, { "type": "cds", "sequence": "contig_1", "start": 493884, "stop": 494366, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03257.18" ], "nt": "GTGTGGGATTTCAAGGGGGTGTGGGCGGATCAAGCTCACAGCGCGGTGTCCGAGTCGCAAGCAGCTACAAGTGGTACCACCACCGCAACAGGGGCTGCCTTACCGGAGCACCCGAATGCTTTGGCGTTTCAGGTGAGTGTGGTGGAAGCGAGTGCTTACAAGCCAAACACGAGCTCCGGCCAAACCCAATCCACTAACAGTTCCCCCTACCTGCATTTGATTCAACCTAAGAAAGTTACCCAATCCGACAAGTTAGACGACGATCTTAAAAACCTGTTGGACCCCAACGAGGTTCGCGCCAGAATGCTCCAATCATTTGGTACAGAAAATTCCACCCAAGCCCAGCCCCAGTCGCTCAAAACAACGACACCGGTATTTGGGGCCATGAGTGGTACCTCGGCAGTGTGCTTAGTGGTGGGGGTACCACGCAGGGCTCAAGCTCAGGTCAATCTGGCGTGGATCTCTCCCCCGTTGAACGGGTGA", "aa": "MWDFKGVWADQAHSAVSESQAATSGTTTATGAALPEHPNALAFQVSVVEASAYKPNTSSGQTQSTNSSPYLHLIQPKKVTQSDKLDDDLKNLLDPNEVRARMLQSFGTENSTQAQPQSLKTTTPVFGAMSGTSAVCLVVGVPRRAQAQVNLAWISPPLNG", "aa_hexdigest": "eb0e73b2fda06397c6bf000d920f8003", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03257.18", "name": "Adhesin_P1_C", "length": 115, "aa_cov": 0.69375, "hmm_cov": 0.9652173913043478, "evalue": 4.0469623892111003e-50, "score": 164.62579345703125, "start": 49, "stop": 159 } ], "seq_stats": { "molecular_weight": 16953.692399999985, "isoelectric_point": 6.258223152160644 }, "id": "APKECPOBEN_894", "locus": "GCA002090215_00878" }, { "type": "cds", "sequence": "contig_1", "start": 494490, "stop": 494741, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_017532788.1", "SO:0001217", "UniParc:UPI0002D77CA5", "UniRef:UniRef100_A0AB38W9C2", "UniRef:UniRef50_A0A0H3DNZ5", "UniRef:UniRef90_A0A0H3DNZ5" ], "nt": "ATGAAATTAGTTAAATTAGCGAGTTTTCACCGCTTTTTAAAACAAGACCAATTGCAACTTGTTCCTTTGTTTAGTCCAAACTTTTTGCAAGTTTTATTGGCGCTTTTCAATAAACCGGTCAATTTATTTAGTGAAAACACAACAATGAAAACAAATAAAAAATACGCCTTTGCGGTGTTACTAATGCAAGCTCAAGCTAAGCTCCCAAATTCATTTAAATTCTTTCTTTCAAAGAAAGGAGGTATGGCTTAA", "aa": "MKLVKLASFHRFLKQDQLQLVPLFSPNFLQVLLALFNKPVNLFSENTTMKTNKKYAFAVLLMQAQAKLPNSFKFFLSKKGGMA", "aa_hexdigest": "a0d78bba7fd026ec85ef7e81cbeb0658", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0002D77CA5", "ncbi_nrp_id": "WP_017532788.1", "uniref100_id": "UniRef100_A0AB38W9C2", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002D77CA5", "RefSeq:WP_017532788.1", "UniRef:UniRef100_A0AB38W9C2" ] }, "ips": { "uniref100_id": "UniRef100_A0AB38W9C2", "uniref90_id": "UniRef90_A0A0H3DNZ5" }, "psc": { "uniref90_id": "UniRef90_A0A0H3DNZ5", "uniref50_id": "UniRef50_A0A0H3DNZ5" }, "pscc": { "uniref50_id": "UniRef50_A0A0H3DNZ5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0H3DNZ5" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9510.348899999994, "isoelectric_point": 10.471680259704591 }, "id": "APKECPOBEN_895", "locus": "GCA002090215_00879" }, { "type": "cds", "sequence": "contig_1", "start": 494909, "stop": 495187, "strand": "+", "frame": 2, "gene": null, "product": "UPF0134 protein MPN_410", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75572", "UniRef:UniRef90_P75572" ], "nt": "GTGAACACGGAATACGTAACGCGTAAAGAGTTCAACGAGTACAAAGACTCAAACGACCAACGACTTATAAAGATTGAAACCACTTTGACCGCCCAAGGTGAACAAATCAACAAATTGACTCAAACTGTTGAAAAACAGGGCGAACAAATTAGAGAACTTCAAGTGGAGCAAAAAGCTCAGGGTGAGCAAATTAAAGCTCAGGGAGAAACCTTAAAGCTAATCTTGCAAACGCTGCAAAAAATGAGTGATCGTTTGGACAAAATTGATCCACCCAAATAG", "aa": "MNTEYVTRKEFNEYKDSNDQRLIKIETTLTAQGEQINKLTQTVEKQGEQIRELQVEQKAQGEQIKAQGETLKLILQTLQKMSDRLDKIDPPK", "aa_hexdigest": "f539444a590abb9fb0a5e5098d17bb04", "start_type": "GTG", "rbs_motif": "AACAA", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 494741, "stop": 495187, "observations": { "mutation": [ 494822 ], "stop": [ 494822 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75572", "query_cov": 1.0, "subject_cov": 0.6216216216216216, "identity": 0.935, "score": 105.0, "evalue": 4.96e-27, "gene_start": 1, "gene_end": 92, "reference_start": 57, "reference_end": 148, "reference_sequence": "MKEKTPFKNEKEFHEMVKKTKKGTFSGWYIIDKDNNSVEFSGKFNRQFKLNKPVITVNTEYVTRKELNEYKDSNDQRLTKIETTLAAQGEQINKLTQTVEKQGEQIRELQVEQKAQGEQIKAQGETLKLILQTLQKMSDRLDKIDPSK" }, "paralog": false, "identity": 0.8918918918918919, "score": 201.0, "evalue": 2.92e-67, "description": "Internal stop codon at 494822. Nonsense mutation around 494822.", "psc": { "uniref90_id": "UniRef90_P75572", "product": "UPF0134 protein MPN_410", "uniref50_id": "UniRef50_P75572", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75572", "UniRef:UniRef50_P75572" ] }, "pscc": { "uniref50_id": "UniRef50_P75572", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75572" ], "product": "UPF0134 protein MPN_204" } }, "id": "APKECPOBEN_896", "locus": "GCA002090215_00880" }, { "type": "cds", "sequence": "contig_1", "start": 495458, "stop": 495751, "strand": "-", "frame": 3, "gene": null, "product": "lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75101", "UniRef:UniRef90_UPI0013300FD7" ], "nt": "ATGGAGCGGAAAGTTGAGTATTTCAATATGGAATATAAAAGCTTTAGTGACTTTTCAGTTAATGCTAGGTTAAGCTATATCTTTAATTGGTATGGGAGTTATTTTGGAGAAAAAAGTTTCACTGCCAATAATGGCGGTAAGCATAATTTCGATCTTTTCCTTAGTATTAAATCTCATTCAAAAAAACAATTTACAGAAAAAAGTTTCATTGTTAAAGATGAAAATTTTCAAGATCAAGATAAAAGTCAACAAATAATTACAGAATGGATTCAGCTTGATCTTTCACTGATTTGA", "aa": "MERKVEYFNMEYKSFSDFSVNARLSYIFNWYGSYFGEKSFTANNGGKHNFDLFLSIKSHSKKQFTEKSFIVKDENFQDQDKSQQIITEWIQLDLSLI", "aa_hexdigest": "3d7eea4762cde666b25b7dc2b6c60885", "start_type": "ATG", "rbs_motif": "AAAA", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 495506, "stop": 496048, "observations": { "mutation": [ 495841 ], "stop": [ 495841 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0013300FD7", "query_cov": 0.8350515463917526, "subject_cov": 0.41968911917098445, "identity": 1.0, "score": 162.0, "evalue": 3.91e-49, "gene_start": 1, "gene_end": 81, "reference_start": 113, "reference_end": 193, "reference_sequence": "MRKKKFLSRFSFSSLFLLCGTLLSACTGIQADLRNLIKETTGKDIDLSKAIKTKEGKKNIITSLKKSYEVNPKDTTKLLLDAWKQSFEEGKLGIAELAFDQATNPTKNSDFKMERKVEYFNMEYKSFSDFSVNARLSYIFNWYGSYFGEKSFTANNGGKHNFDLFLSIKSHSKKQFTEKSFIVKDENFQDQDK" }, "paralog": false, "identity": 0.9166666666666666, "score": 330.0, "evalue": 2.37e-117, "description": "Internal stop codon at 495841. Nonsense mutation around 495841.", "psc": { "uniref90_id": "UniRef90_UPI0013300FD7", "product": "lipoprotein", "uniref50_id": "UniRef50_P75101", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0013300FD7", "UniRef:UniRef50_P75101" ] }, "pscc": { "uniref50_id": "UniRef50_P75101", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75101" ], "product": "Uncharacterized protein MPN_012" } }, "id": "APKECPOBEN_897", "locus": "GCA002090215_00881" }, { "type": "cds", "sequence": "contig_1", "start": 495839, "stop": 496087, "strand": "-", "frame": 3, "gene": null, "product": "putative lipoprotein MPN_467", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75316" ], "nt": "ATGCGCAAAAAGAAATTTTTGTCAAGGTTTGCTTTTGGTTCTTTATTTTTGTTATGTGGAACAATTTTAAGCGCTTGCACTGGTATCCAAGCTGATCTTAGAAATCTTATTAAAGAAGCCACCGGTAAAGACATTGATTTGTCTAAATCAATTAAAACTACAGATGGTAAAAAGAACATAATAACTTCTTCAAAGAAGTCTTACGAAGTTAATCCGAAAGATACAACAAAATTGTTGTTAGAAGCATGA", "aa": "MRKKKFLSRFAFGSLFLLCGTILSACTGIQADLRNLIKEATGKDIDLSKSIKTTDGKKNIITSSKKSYEVNPKDTTKLLLEA", "aa_hexdigest": "9dc60504a37f775dd4f66f1cea6a9195", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75316", "query_cov": 1.0, "subject_cov": 0.8118811881188119, "identity": 0.732, "score": 114.0, "evalue": 1.37e-31, "valid": false, "product": "Uncharacterized lipoprotein MPN_467", "uniref50_id": "UniRef50_P75316", "go_ids": [ "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75316", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75316" ], "product": "Uncharacterized lipoprotein MPN_467" }, "genes": [], "id": "APKECPOBEN_898", "locus": "GCA002090215_00882" }, { "type": "cds", "sequence": "contig_1", "start": 496594, "stop": 497046, "strand": "+", "frame": 1, "gene": null, "product": "putative protein MPN_412", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75321", "UniRef:UniRef50_UPI001375E5B8", "UniRef:UniRef90_Q9EXD5", "UniRef:UniRef90_UPI001375E5B8" ], "nt": "GTGGTGTACTATGAGCAGTTGCAGCGGGGGTTGGGCTTACCACAGCAGCGAACCGAGAATGGTCAAAATACTTCAACCACCGGGGCAATGTTTGGCTTGAAGGTGAAGGATGCCCAAAAAGACGACCAGAAGTCCTCGGAATATTTAAGTGGGGAGGCGGGAAGTCAAGCGGGTGGATCCACCCAAGGTACTTCCACCACCACCCAACGTAGGGGTTCGTCAAATGAAAACAAAGTCAAGGTGTTGCAGGTGGCGATGAAAAAGAAATCGGACTCGGAGGACAATGGTCAGATCGAACTTGAAACCAACAACCTCGCCAACGCCCCGATTAAACGGGGCTCCAATAACAACCAGCAAGTCCAACTCAAGGCGGACGATTTTGGTACCACCTCCAGTTCGGAATCTGGTCAATCAGGTACTCAAGGATCCACCCCAAGTAATCCGGGACCCTGA", "aa": "MVYYEQLQRGLGLPQQRTENGQNTSTTGAMFGLKVKDAQKDDQKSSEYLSGEAGSQAGGSTQGTSTTTQRRGSSNENKVKVLQVAMKKKSDSEDNGQIELETNNLANAPIKRGSNNNQQVQLKADDFGTTSSSESGQSGTQGSTPSNPGP", "aa_hexdigest": "a116cbbde6aec246ef7f92eda764ca25", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001375E5B8", "query_cov": 0.86, "subject_cov": 0.8486842105263158, "identity": 0.992, "score": 234.0, "evalue": 4.42e-77, "valid": true, "uniref50_id": "UniRef50_UPI001375E5B8" }, "pscc": { "uniref50_id": "UniRef50_UPI001375E5B8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001375E5B8" ] }, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 496594, "stop": 497349, "observations": { "mutation": [ 497044, 497206, 497221, 497245 ], "stop": [ 497044, 497206, 497221, 497245 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9EXD5", "query_cov": 0.9, "subject_cov": 0.43973941368078173, "identity": 1.0, "score": 244.0, "evalue": 1.08e-78, "gene_start": 1, "gene_end": 135, "reference_start": 1, "reference_end": 135, "reference_sequence": "MVYYEQLQRGLGLPQQRTENGQNTSTTGAMFGLKVKDAQKDDQKSSEYLSGEAGSQAGGSTQGTSTTTQRRGSSNENKVKVLQVAMKKKSDSEDNGQIELETNNLANAPIKRGSNNNQQVQLKADDFGTTSSSESGQSGTQGSTPSNPGPWTPWLTTEQIHNDPAKFAASILILYDAPYARNRTAIDRVDHLDPKVMTANYPPSWRTPKWNHHGLWDWKARDVLLQTTGFFNPRRHPEWFDGGQTVADNEKTGFDVDNSENTKQGFQKEADSDKSAPIALPFEAYFANIGNLTWFGQALLVFGICLS" }, "paralog": false, "identity": 0.9083665338645418, "score": 449.0, "evalue": 5.03e-162, "description": "Internal stop codon at 497044, 497206, 497221, 497245. Nonsense mutation around 497044, 497206, 497221, 497245.", "psc": { "uniref90_id": "UniRef90_Q9EXD5", "product": "Uncharacterized protein MPN_412", "uniref50_id": "UniRef50_P75321", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9EXD5", "UniRef:UniRef50_P75321" ] }, "pscc": { "uniref50_id": "UniRef50_P75321", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75321" ], "product": "Uncharacterized protein MPN_462" } }, "id": "APKECPOBEN_899", "locus": "GCA002090215_00883" }, { "type": "cds", "sequence": "contig_1", "start": 497263, "stop": 497517, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF12378.14" ], "nt": "TTGCTCCAAACCACCGGGTTCTTCAACCCGCGCCGCCACCCCGAGTGGTTTGATGGCGGGCAGACGGTCGCGGATAACGAAAAGACCGGGTTTGATGTGGATAACTCCGAAAACACCAAGCAGGGCTTTCAAAAGGAAGCTGACTCCGACAAGTCGGCCCCGATCGCCCTCCCGTTTGAAGCGTACTTCGCCAACATTGGCAACCTCACCTGGTTCGAGCAAGCGCTTTTGGTGTTTGGGATTTGTTTGTCTTAA", "aa": "MLQTTGFFNPRRHPEWFDGGQTVADNEKTGFDVDNSENTKQGFQKEADSDKSAPIALPFEAYFANIGNLTWFEQALLVFGICLS", "aa_hexdigest": "451778bce0a189a6b072b668f7a1372b", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF12378.14", "name": "P1_N", "length": 77, "aa_cov": 0.9166666666666666, "hmm_cov": 0.6623376623376623, "evalue": 1.9458650160195823e-17, "score": 58.95265197753906, "start": 4, "stop": 80 } ], "seq_stats": { "molecular_weight": 9421.290499999997, "isoelectric_point": 4.418060874938965 }, "id": "APKECPOBEN_900", "locus": "GCA002090215_00884" }, { "type": "cds", "sequence": "contig_1", "start": 497662, "stop": 498039, "strand": "+", "frame": 1, "gene": null, "product": "Putative MgpC-like protein MPN_366", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75415", "UniRef:UniRef90_A0AB38W808" ], "nt": "GTGGAGGATACCAAGTTGGTGGCGCTGGTCCGTTCAGCGTTGGTCAGGGTGAAGTTTCAAGATAGTTCGAGCCAACAAGGCACTGAGAGTAATGGCCAAGACCAAAATGCCCTTAGTTTTGATACCACCAAGTCCCAACAAGCCTTACAGCAAGCTGGCGATCAATCAGGAATTTCATCTAATTCCACCCAAGAAGACTTCGCCAGCTACATCCTCATCTTTAAAGCCGCCCCGAAGGCCACGTGGGTGTTTGAACGGAAGATTAAGTTGGCGTTGCCCTACGTTAAGCAGGAAAGTCAGGGTTCCGACGATCAGGTTCCAATGGTAAGGGCTCCCTCTACAAAACCCTCCAAGACCTCCTCGTCGAACAACCCGTGA", "aa": "MEDTKLVALVRSALVRVKFQDSSSQQGTESNGQDQNALSFDTTKSQQALQQAGDQSGISSNSTQEDFASYILIFKAAPKATWVFERKIKLALPYVKQESQGSDDQVPMVRAPSTKPSKTSSSNNP", "aa_hexdigest": "aad2ff1c411dd3705c242c7ee1115381", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 497611, "stop": 498297, "observations": { "insertion": [ 498241 ], "deletion": [ 497977 ], "mutation": [ 497653 ], "stop": [ 497653, 498124, 498232 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB38W808", "query_cov": 0.808, "subject_cov": 0.42616033755274263, "identity": 0.98, "score": 182.0, "evalue": 1.19e-55, "gene_start": 5, "gene_end": 105, "reference_start": 21, "reference_end": 121, "reference_sequence": "MRAAHLHTSYELVDWKVGQNKLVALVRSALVRVKFQDSSSQQGTESNGQDQNALSFDTTKSQQALQQAGDQSGTSSNSTQEDFASYILIFKAAPRATWVFERKIKLALPYVKQESQGSDDQGSNGKGSLYTTLKDLLVEQPVTPYTPNAGLARVNGVAQDKVHGLESGWDNQRSQKNLTNNPGPKAVTGFKLDKGRAYRKLNESWPVSNPSIRPRLARGKVPQNEKRRRQMLRVMLP" }, "paralog": false, "identity": 0.8508771929824561, "score": 358.0, "evalue": 2.51e-127, "description": "Internal stop codon at 497653, 498124, 498232. Frameshift due to insertion around 498241. Frameshift due to deletion around 497977. Nonsense mutation around 497653.", "psc": { "uniref90_id": "UniRef90_A0AB38W808", "uniref50_id": "UniRef50_P75415", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB38W808", "UniRef:UniRef50_P75415" ] }, "pscc": { "uniref50_id": "UniRef50_P75415", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75415" ], "product": "Putative MgpC-like protein MPN_366" } }, "id": "APKECPOBEN_901", "locus": "GCA002090215_00885" }, { "type": "cds", "sequence": "contig_1", "start": 498036, "stop": 498125, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGACCCCTTACACGCCCAATGCGGGGTTAGCCCGGATGAATGGGGTTGCTCAGGACAAGGTGCATGGGTTGGAACAAACCACTCAATGA", "aa": "MTPYTPNAGLARMNGVAQDKVHGLEQTTQ", "aa_hexdigest": "47b2022e32c8fb70a62a7225832ee355", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3129.4827999999998, "isoelectric_point": 6.505302238464354 }, "id": "APKECPOBEN_902", "locus": "GCA002090215_00886" }, { "type": "cds", "sequence": "contig_1", "start": 498336, "stop": 498563, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAGCTCAACCGAGAGTCAAATGGCTGCAGTCACAGACAGTCAGCAATCAGGTCATAACAGTGGATTGGTATCGCTTGCCCAGCGCTCCACTACTGTTGCTGTCCAAAAAAGTGACTCTTCTGGTTCTCAAGGCCAAGGCACAACCGACAACAAATTCCAAAAATATTTAAACACCGCCCAGGCCTTGCACCAGATGGGGGTGATAGTGCCATCTTTGGAAACGTGA", "aa": "MSSTESQMAAVTDSQQSGHNSGLVSLAQRSTTVAVQKSDSSGSQGQGTTDNKFQKYLNTAQALHQMGVIVPSLET", "aa_hexdigest": "01a905cfd228e3b6c2671a39b4f01a08", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7844.479600000001, "isoelectric_point": 5.985339164733887 }, "id": "APKECPOBEN_903", "locus": "GCA002090215_00887" }, { "type": "cds", "sequence": "contig_1", "start": 498822, "stop": 499499, "strand": "+", "frame": 3, "gene": "mgpC", "product": "MgpC family cytadherence protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002246AD21", "UniRef:UniRef90_UPI002246AD21" ], "nt": "TTGAACTGGGGCACCGACAAGCAGAAGCAGTTTGTGGAGAATCAGCTTGGTTTTAAAGATGACTCAAATTCTTCCTTGACAAACTTCAAGAGTCAAGGCTTAACTCAGCCAGCTTACCTCATCGCCGGCCTTGACGTTGTCCAAGACCACCTCGTCTTTGCGGCCTTCAAAGCGGGCGCGGTGGGGTATGATATGACGACTGATTCGAACGCTTCCACCAAAGACCAAGCGCTCGCCTGGTCGACCACGGCCGGGTTGGACAGTGCTGGGGGGTACAACAACCTGGTGGAAAACACGGCCGGACTGAACGGCCCGATTAACGACTTGTTTACCCTGCTCGACACCTTTGCCTATGTGACCCCGGTGAGTGGGATGAAAGGGGGGAGTCAGAATAACGAAGAAGTGCAGACCAAGTATCCCGTTAAGGATGATAGTAAGGCTTCCGCCAAAATTGCGTCCTTAATTAATGCCAGTCCACTCAACAGTTATGGGGATGATGGGGTGACCGTGTTTGATGCGTTGGGCCTTAACTTTAACTTTAAGTTGGACGAGGCGCGCTTGCCATCGCGCACCGACCAACTGCTTGTGTATGGGATTGTAAACGAAAGTGAACTGAAGTCCGCGCGCGAAAATGCCCAGTCGACCTCCGATGATAATTCAAACACCAAAGTCAAGTGA", "aa": "MNWGTDKQKQFVENQLGFKDDSNSSLTNFKSQGLTQPAYLIAGLDVVQDHLVFAAFKAGAVGYDMTTDSNASTKDQALAWSTTAGLDSAGGYNNLVENTAGLNGPINDLFTLLDTFAYVTPVSGMKGGSQNNEEVQTKYPVKDDSKASAKIASLINASPLNSYGDDGVTVFDALGLNFNFKLDEARLPSRTDQLLVYGIVNESELKSARENAQSTSDDNSNTKVK", "aa_hexdigest": "5fc875747bbe0fa1ac9a74df200348b1", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002246AD21", "query_cov": 0.8711111111111111, "subject_cov": 0.8521739130434782, "identity": 0.985, "score": 370.0, "evalue": 1.53e-128, "valid": true, "product": "MgpC family cytadherence protein", "uniref50_id": "UniRef50_UPI002246AD21" }, "pscc": { "uniref50_id": "UniRef50_UPI002246AD21", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002246AD21" ], "product": "MgpC family cytadherence protein" }, "genes": [ "mgpC" ], "id": "APKECPOBEN_904", "locus": "GCA002090215_00888" }, { "type": "cds", "sequence": "contig_1", "start": 499849, "stop": 500304, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTAAAAAGAAAAAAACTGCTCCAGGGTTTCTTGAAATTTTTACCGCTTATCATTCCCGCAACCATTTTTGTTAGCTGTGCAAGACGTGAAAGTAATCACCTCATCTTCAACATTTCACTCGATCACGACGCTGATGCTTCTATTAGTAAGTTCTTTGAACTTTACTCTAATAATTTAAGTAAAAAACTAGATAAAAAGGTTACCGTTAGCTTCAACATTATTGATGACTCTTTTACCAAAATTAGCAACATTCAAACTGCTAAAGCTGACTTTGCTTTCGTTAATTCTCAAAGTATTAAAGATAACGGAATAGAAGAATTTAATCTTATTCTTCAAACTCAAACTGATGCATTTAAGGAAGACACCAATTTAGATTATTACAGTGACGGTCAGTTGAAATCTAAAGCAGAAAAAATGACCACTTTATTTTCAAAAACGCCATATAAAGATTGA", "aa": "MLKRKKLLQGFLKFLPLIIPATIFVSCARRESNHLIFNISLDHDADASISKFFELYSNNLSKKLDKKVTVSFNIIDDSFTKISNIQTAKADFAFVNSQSIKDNGIEEFNLILQTQTDAFKEDTNLDYYSDGQLKSKAEKMTTLFSKTPYKD", "aa_hexdigest": "f155a15e08a15f8bec8bf13ff7af3d93", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17277.55439999998, "isoelectric_point": 8.557678413391116 }, "id": "APKECPOBEN_905", "locus": "GCA002090215_00889" }, { "type": "cds", "sequence": "contig_1", "start": 500464, "stop": 500676, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATTATGGCATCGGTCATGGCTCAAGTGGTTCAGCTGGAAAGTTCCAACTACCTGATCTTTTATTAAGAAAGCACTTTGGCTCTAGTTACCCCGGTTTGCAAAATGCAATTAACCAAAACCCTGATAAGTTTGCTAATGTTAGAGGTCGGGAAATAGGTAGAGACAATAAAATCAAAATTGTCTTTGATGATGCTAATTCCTTTGCTTGA", "aa": "MNYGIGHGSSGSAGKFQLPDLLLRKHFGSSYPGLQNAINQNPDKFANVRGREIGRDNKIKIVFDDANSFA", "aa_hexdigest": "469fe2f99e433fdb535c27c8a25b915a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7651.465000000001, "isoelectric_point": 9.598198127746581 }, "id": "APKECPOBEN_906", "locus": "GCA002090215_00890" }, { "type": "cds", "sequence": "contig_1", "start": 500800, "stop": 500991, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTGTATGACATTGGCATTGTAAGTGATACTTTAAGTGATCGCTATCAAAAAGCAATTGCTGAAGTTTTTGTGGAACTAGCTAAAACAAAACAAGATATTTATGGCCCTTCATATGGTTACAACGGTTACAATTTGATTACGGACCCTAACAAAGAAATCTTGGATGTAATTCATAAAACATATGGTTAA", "aa": "MLYDIGIVSDTLSDRYQKAIAEVFVELAKTKQDIYGPSYGYNGYNLITDPNKEILDVIHKTYG", "aa_hexdigest": "d5362b939a7c09d9e9b55ef7033bf097", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7161.999900000001, "isoelectric_point": 4.749375343322753 }, "id": "APKECPOBEN_907", "locus": "GCA002090215_00891" }, { "type": "cds", "sequence": "contig_1", "start": 500984, "stop": 501286, "strand": "+", "frame": 2, "gene": "p29", "product": "putative ABC transporter ATP-binding protein p29", "db_xrefs": [ "COG:COG1120", "COG:PH", "GO:0005524", "GO:0016887", "SO:0001217", "UniRef:UniRef50_P47532", "UniRef:UniRef90_P75370" ], "nt": "ATGGTTAATCCAGTCTTAGTTTTTGATCAAGTTTCCCTTAGATATAACGGAGCACCACTTTTAGAAAACATTAACTTTACAATTTCACCTGGTGAACACATTTGCTTGTTGGGTAAATCTGGTGTTGGTAAAACTAGTCTTTTAAACTGCATTACTAACACCAAAACAATTTCGAAGGGAACCATTTATTTCAATGGTATTGCTTCTAATAACAAGGATTACAAACAATTAAAAAAACAGTTCAGTTTTTTAGATCAAGTACCTAATTTAATCGACACTGACTTCGTTTATGACGCCATTTGA", "aa": "MVNPVLVFDQVSLRYNGAPLLENINFTISPGEHICLLGKSGVGKTSLLNCITNTKTISKGTIYFNGIASNNKDYKQLKKQFSFLDQVPNLIDTDFVYDAI", "aa_hexdigest": "73fab8a3d6b507916cc268b7dcc91f8b", "start_type": "ATG", "rbs_motif": null, "genes": [ "p29" ], "truncated": "3-prime", "pseudogene": { "start": 500984, "stop": 501589, "observations": { "mutation": [ 501284, 501314 ], "stop": [ 501284, 501314 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75370", "query_cov": 1.0, "subject_cov": 0.4098360655737705, "identity": 1.0, "score": 203.0, "evalue": 1.69e-64, "gene_start": 1, "gene_end": 100, "reference_start": 1, "reference_end": 100, "reference_sequence": "MVNPVLVFDQVSLRYNGAPLLENINFTISPGEHICLLGKSGVGKTSLLNCITNTKTISKGTIYFNGIASNNKDYKQLKKQFSFLDQVPNLIDTDFVYDAIWREAKNNLKWWQRLFLVEPQSLREQIIQILEEVNLKEYVTYIIKDLSGGQKQRVEVAKLFFANSQVLLVDEPTTGLDLINAHKIMELIIQFARQKAMTLIFVTHDVEFALKYSDRIIALKNKALVLDQATNKLTKQKLMQIYHD" }, "paralog": false, "identity": 0.9900497512437811, "score": 389.0, "evalue": 6e-140, "description": "Internal stop codon at 501284, 501314. Nonsense mutation around 501284, 501314.", "psc": { "uniref90_id": "UniRef90_P75370", "gene": "p29", "product": "Probable ABC transporter ATP-binding protein p29", "uniref50_id": "UniRef50_P47532", "cog_id": "COG1120", "cog_category": "PH", "go_ids": [ "GO:0005524", "GO:0016887" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75370", "UniRef:UniRef50_P47532", "COG:COG1120", "COG:PH", "GO:0005524", "GO:0016887" ] }, "pscc": { "uniref50_id": "UniRef50_P47532", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47532" ], "product": "Probable ABC transporter ATP-binding protein p29" } }, "id": "APKECPOBEN_908", "locus": "GCA002090215_00892" }, { "type": "cds", "sequence": "contig_1", "start": 501332, "stop": 501718, "strand": "+", "frame": 2, "gene": "p29", "product": "putative ABC transporter ATP-binding protein p29", "db_xrefs": [ "COG:COG1120", "COG:PH", "GO:0005524", "GO:0016887", "SO:0001217", "UniRef:UniRef50_P47532", "UniRef:UniRef90_P75370" ], "nt": "GTGGAACCTCAATCGCTAAGAGAACAAATTATCCAAATCCTTGAAGAGGTTAACCTAAAGGAATATGTCACTTACATTATTAAGGACTTGTCAGGTGGTCAAAAACAACGTGTGGAAGTAGCTAAATTGTTCTTTGCCAATTCCCAAGTTTTATTAGTTGATGAGCCCACTACTGGACTTGATTTAATCAATGCGCACAAAATCATGGAACTAATTATCCAATTTGCGCGCCAAAAAGCAATGACCTTAATCTTTGTGACCCATGATGTTGAGTTTGCGCTCAAATACAGTGACCGTATTATTGCTCTAAAAAATAAGGCATTAGTTTTAGATCAAGCTACAAATAAGCTTACCAAACAAAAGTTAATGCAAATTTATCATGACTAG", "aa": "MEPQSLREQIIQILEEVNLKEYVTYIIKDLSGGQKQRVEVAKLFFANSQVLLVDEPTTGLDLINAHKIMELIIQFARQKAMTLIFVTHDVEFALKYSDRIIALKNKALVLDQATNKLTKQKLMQIYHD", "aa_hexdigest": "60cc4c74ec63c45c48cf5aef54fc3234", "start_type": "GTG", "rbs_motif": null, "genes": [ "p29" ], "truncated": "5-prime", "pseudogene": { "start": 501032, "stop": 501718, "observations": { "mutation": [ 501284, 501314 ], "stop": [ 501284, 501314 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75370", "query_cov": 1.0, "subject_cov": 0.5245901639344263, "identity": 0.992, "score": 240.0, "evalue": 1.98e-78, "gene_start": 1, "gene_end": 128, "reference_start": 117, "reference_end": 244, "reference_sequence": "MVNPVLVFDQVSLRYNGAPLLENINFTISPGEHICLLGKSGVGKTSLLNCITNTKTISKGTIYFNGIASNNKDYKQLKKQFSFLDQVPNLIDTDFVYDAIWREAKNNLKWWQRLFLVEPQSLREQIIQILEEVNLKEYVTYIIKDLSGGQKQRVEVAKLFFANSQVLLVDEPTTGLDLINAHKIMELIIQFARQKAMTLIFVTHDVEFALKYSDRIIALKNKALVLDQATNKLTKQKLMQIYHD" }, "paralog": false, "identity": 0.9912280701754386, "score": 441.0, "evalue": 7.07e-160, "description": "Internal stop codon at 501284, 501314. Nonsense mutation around 501284, 501314.", "psc": { "uniref90_id": "UniRef90_P75370", "gene": "p29", "product": "Probable ABC transporter ATP-binding protein p29", "uniref50_id": "UniRef50_P47532", "cog_id": "COG1120", "cog_category": "PH", "go_ids": [ "GO:0005524", "GO:0016887" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75370", "UniRef:UniRef50_P47532", "COG:COG1120", "COG:PH", "GO:0005524", "GO:0016887" ] }, "pscc": { "uniref50_id": "UniRef50_P47532", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47532" ], "product": "Probable ABC transporter ATP-binding protein p29" } }, "id": "APKECPOBEN_909", "locus": "GCA002090215_00893" }, { "type": "cds", "sequence": "contig_1", "start": 501915, "stop": 502157, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGGCTCTTACCTACTGCAAACCATTTTTTATGTCGTGAGTGGTAGTATTTTGGGATTTGTCATAGCCCTGTGGTTTTCGTACCTCACTGCCTTTAAAATCCAGCCACTTTACATCGCTTTACCAACTCGCTTATTCACCATTTTTCTGCGTTCCTTTCCAGTACTTGTGTTTGCTTTTTTGTTTAACAATTTGTTTAACAAACAACTAACAGCAACCTTAACGATTACCTGGTTTAGTTGA", "aa": "MGSYLLQTIFYVVSGSILGFVIALWFSYLTAFKIQPLYIALPTRLFTIFLRSFPVLVFAFLFNNLFNKQLTATLTITWFS", "aa_hexdigest": "3275dc4062964590309d04ffad3c665b", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9256.969499999988, "isoelectric_point": 9.994614219665529 }, "id": "APKECPOBEN_910", "locus": "GCA002090215_00894" }, { "type": "cds", "sequence": "contig_1", "start": 502344, "stop": 502562, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAGTATTGCTGGCATTACTGGAATTGGTGAGCTCATTGCCACACCTCTAGGGGGCACAGTGCAGCTTAACTTAGTTTTAATACCGATGTTAACTTTGATCGGCTTTTTACTGTTTTTAGAAGCGAGTGTTTTTCTGTTAACTAAGTTTGTTTTACAAAAACAGAGCCAAGCCGGTGATTACTTTTTACAAGCTAAAACACTGCAAAAGAGAAAGTGA", "aa": "MSIAGITGIGELIATPLGGTVQLNLVLIPMLTLIGFLLFLEASVFLLTKFVLQKQSQAGDYFLQAKTLQKRK", "aa_hexdigest": "f988381b71b2440f54ca07680c3befa8", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7822.357799999995, "isoelectric_point": 9.69799518585205 }, "id": "APKECPOBEN_911", "locus": "GCA002090215_00895" }, { "type": "cds", "sequence": "contig_1", "start": 502572, "stop": 503075, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTTACATCCTAGCTTTGGTTTTAGCGGCTTTTACCTTGGCTAATTTAGTACAGTTGGACTATACGGTTAAAGCGCCAGGGTTTGTGGCTGACTTTTTTAAACAGTTCTTTCAAACAAAAACAGCTTTTTTAATTAGTGAAGACGCTAACATTAACCCACTTTTAATGTTGTTAAAACTCACAACTCAAGCGATTAGTTTAATTACCTTAGTGTTTGTGTTAGCACTCTTGTTTGGCTTTTTAGCATCAAAGCTGTTTTCCACTATAACCTCAATTAGCTTAAAACTGTTACTTTTAGTGATTAGGGTAATTCCCAGTGTCTTACTGTTCCGCTTATTTGATCCAATTATTTTTCGCCCAGAAACTACCATTATCTTTGTTTTAGCAATCCACAGTGCTGCTAGTTATGGTCAACTGATAACGATTAACTTTGATAATGCCAACGAAGGGGTAATTAACAACATGCAGAACCACGGCTTTAGTAGGTTTTACATCTTGTGA", "aa": "MIYILALVLAAFTLANLVQLDYTVKAPGFVADFFKQFFQTKTAFLISEDANINPLLMLLKLTTQAISLITLVFVLALLFGFLASKLFSTITSISLKLLLLVIRVIPSVLLFRLFDPIIFRPETTIIFVLAIHSAASYGQLITINFDNANEGVINNMQNHGFSRFYIL", "aa_hexdigest": "af800cc8c3311ccb8242ea5532686aef", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18696.141499999987, "isoelectric_point": 9.048734092712401 }, "id": "APKECPOBEN_912", "locus": "GCA002090215_00896" }, { "type": "cds", "sequence": "contig_1", "start": 503157, "stop": 503321, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTTGGGATCTTTGGTGGTTCGATTATTGGGGGGCGAATTAACAATTTCTTTGAGCGAGCACAGTACTCTGAATTGGGCACAATAACCTTACCCTTAATGGTTTACTTAATGGTGTTTGAAGTCATTTTAATGGCAGTACGGTTAAACAAACTCAAAGTGTGA", "aa": "MFGIFGGSIIGGRINNFFERAQYSELGTITLPLMVYLMVFEVILMAVRLNKLKV", "aa_hexdigest": "358558c19e8255f9d64aafba5048e67b", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6112.340400000001, "isoelectric_point": 9.518902015686034 }, "id": "APKECPOBEN_913", "locus": "GCA002090215_00897" }, { "type": "cds", "sequence": "contig_1", "start": 503340, "stop": 503762, "strand": "-", "frame": 2, "gene": "ruvX", "product": "Holliday junction resolvase RuvX", "db_xrefs": [ "COG:COG0816", "COG:JLK", "EC:3.1.-.-", "EC:3.1.21.10", "GO:0000967", "GO:0004518", "GO:0005829", "GO:0006364", "RefSeq:WP_010874774.1", "SO:0001217", "UniParc:UPI00001395BD", "UniRef:UniRef100_P57114", "UniRef:UniRef50_Q9ZB76", "UniRef:UniRef90_P57114" ], "nt": "GTGCAGTACATTTTAGGCATTGATTTTGGTTTGAAGAGAATTGGTACAGCACTAGTCAATACCATTGACCGCTTTCCAAGTCCCTTCCGCGTGTTTGCGGTACAAAATAATTTGCAACAAGCCGTTAATACCCTTTTTAAGGATTTAAAGCAAGCTGGTTATGAATTAGTGCAAATAGTGATTGGCTTTCCCCACTTCCACTACCAGTCAAGTATCCAAGTGAGCATTCACAAGTTTGTGGAACTAATAAAAACCCGCTTTAATGTACCAGTAACACTCATTGATGAAAGTGGTACTACGAGTGAGGTAAAAGCTAACTTGCAGGAATTAGGCTTAAAAAACAGAACCTTTAAAAAGGCCAAGGACACCTTAGCGGCTACCTTAATCTTAGAGCGTTTCTTAAACCAACAAAAACCCAATTAA", "aa": "MQYILGIDFGLKRIGTALVNTIDRFPSPFRVFAVQNNLQQAVNTLFKDLKQAGYELVQIVIGFPHFHYQSSIQVSIHKFVELIKTRFNVPVTLIDESGTTSEVKANLQELGLKNRTFKKAKDTLAATLILERFLNQQKPN", "aa_hexdigest": "b415839069e543d9a2e939bc66205194", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001395BD", "ncbi_nrp_id": "WP_010874774.1", "uniref100_id": "UniRef100_P57114", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001395BD", "RefSeq:WP_010874774.1", "UniRef:UniRef100_P57114" ] }, "ips": { "uniref100_id": "UniRef100_P57114", "uniref90_id": "UniRef90_P57114" }, "psc": { "uniref90_id": "UniRef90_P57114", "gene": "ruvX", "product": "Holliday junction resolvase RuvX", "ec_ids": [ "3.1.-.-", "3.1.21.10" ], "uniref50_id": "UniRef50_Q9ZB76", "cog_id": "COG0816", "cog_category": "JLK", "go_ids": [ "GO:0000967", "GO:0004518", "GO:0005829", "GO:0006364" ] }, "pscc": { "uniref50_id": "UniRef50_Q9ZB76", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9ZB76" ], "product": "Putative pre-16S rRNA nuclease" }, "genes": [ "ruvX" ], "id": "APKECPOBEN_914", "locus": "GCA002090215_00898" }, { "type": "cds", "sequence": "contig_1", "start": 503762, "stop": 504250, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAACAAGCAAAAAACTGCTTTAACTAAGCAACTAAATCACTTTCACTTGCCGCAAGTAATTACTGATTTACGTAAATGCCAAGCTTTACTAAATGAACTCAAAATAACGGTTAATGAACTGAAAACGGAAGACTTTAAGTGGAAGCAAAAGCAGTTAGCGGAAAAGATCAAACAGGAATTGTTAGAGCTAGCAAAGCAAGACAAAGCTTATGTTCTCGCTAGCTTTGCAGCAGTTGATCCGAAACTGTTATCACAAGTAGCCCAAGCAGTCTTGAACCAACACAAAAACAAGCTGTTTGTACTCCTCAACCAATTCAACAACAGTCCGTCCTTTATGTTATTAGGGCAAGATGTTTCTAAATGTATTCAGCTTTTAAAAGCTCACTTTGAACTTAAGGGTGGGGGTAGTAATAACTTCTTTAGGGGTTCGTTTAACGAGTCGGTGGATGTAAGTAAACTACAAGCTATCCTCGATACCCTTCAATAG", "aa": "MNKQKTALTKQLNHFHLPQVITDLRKCQALLNELKITVNELKTEDFKWKQKQLAEKIKQELLELAKQDKAYVLASFAAVDPKLLSQVAQAVLNQHKNKLFVLLNQFNNSPSFMLLGQDVSKCIQLLKAHFELKGGGSNNFFRGSFNESVDVSKLQAILDTLQ", "aa_hexdigest": "858125cfe8d0ecc94c0f72852f2ab4f4", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18422.22019999999, "isoelectric_point": 9.439734840393069 }, "id": "APKECPOBEN_915", "locus": "GCA002090215_00899" }, { "type": "cds", "sequence": "contig_1", "start": 504371, "stop": 504622, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF07973.19" ], "nt": "GTGGAACACAGGATTCGACAAATTATTCAACAAGACATTCAGCGCGAAGAGATCTTTACTGATCTAGAGGGTAGTCAAAAACTTAATGCGATTGCCTACTTTGAAGAGGAGTATTCGAACCACGAGTTGTTACGGGTAATTCGCTTTGGTGATTTTAGTGTCGAGCTTTGTGGTGGTACCCATGTGGAACACACTGGTTTAATTGAGAACTGTTTTATTACTGATTACTATGCTCGTGGCACAGGGCGTTGA", "aa": "MEHRIRQIIQQDIQREEIFTDLEGSQKLNAIAYFEEEYSNHELLRVIRFGDFSVELCGGTHVEHTGLIENCFITDYYARGTGR", "aa_hexdigest": "906ac5d966814cf2bbcd1d4b368bde07", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07973.19", "name": "tRNA_SAD", "length": 37, "aa_cov": 0.4457831325301205, "hmm_cov": 1.0, "evalue": 5.0210651253896205e-14, "score": 47.535125732421875, "start": 44, "stop": 80 } ], "seq_stats": { "molecular_weight": 9751.746899999991, "isoelectric_point": 4.915629386901855 }, "id": "APKECPOBEN_916", "locus": "GCA002090215_00900" }, { "type": "cds", "sequence": "contig_1", "start": 504638, "stop": 505183, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAACAGCACCGTTTGATCTCCAAACAAAACAGTAATACGTTGAGCTTTGAAAAGCAAAATGAACACTTAGTGAACTTTAAAACAGCGAGTGAGTTCTTATACGAGGCTAATGAGATCACCGCCAAGGTCATTGGGCTGTTTGATGAACAGTACCAACCGGTGCAAAAACTGCACAACCAAAGTGGTTATGTGGTGTTTGACCAAACGGTGCTGTATGCCACATCAGGCGGTCAGCGTTATGATGAGGGGTACTGTATTAACCACAGCCAAAATGATCAAAGAGTAAGCTTTCAGGGCGTGTTTAAAGGCCCTAATAAACAACACTTCCACTTCTTTTTAACTGGTAGTTTTCAACTAGGTGACAAAGTGATCCTAGTACATGACGGTAAGTGGCGCCAACTAGTGAAAAACAACCACAGTTTAGAACACTTACTGCACGCGGCATTGCAAAATGAAATTGACCCCTTGATTAAACAAGACGGCGCCTTTAAGTCAGCGCAAAAAGCTACCATTGACTTTAATTTTTCGCGAGCCTTAACCTGA", "aa": "MEQHRLISKQNSNTLSFEKQNEHLVNFKTASEFLYEANEITAKVIGLFDEQYQPVQKLHNQSGYVVFDQTVLYATSGGQRYDEGYCINHSQNDQRVSFQGVFKGPNKQHFHFFLTGSFQLGDKVILVHDGKWRQLVKNNHSLEHLLHAALQNEIDPLIKQDGAFKSAQKATIDFNFSRALT", "aa_hexdigest": "124d6cc5f82bb9463262ee210b2392d6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 20816.012600000013, "isoelectric_point": 6.79125919342041 }, "id": "APKECPOBEN_917", "locus": "GCA002090215_00901" }, { "type": "cds", "sequence": "contig_1", "start": 505202, "stop": 505333, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTGTTTGAAAAGAGTATTGCTCATAATGCACTCCAACCCCAAGTAGCCTTCCAGTTGTACGAAACCTATGGCTTTCCGATTGAAATGATTAAGGAACTGGTGGATAAGCGCCAACTCCAGGTAGACTGA", "aa": "MLFEKSIAHNALQPQVAFQLYETYGFPIEMIKELVDKRQLQVD", "aa_hexdigest": "43c654cb30cd3ccdc08f4e718c0dfadb", "start_type": "ATG", "rbs_motif": "AACAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5082.846800000002, "isoelectric_point": 5.09427433013916 }, "id": "APKECPOBEN_918", "locus": "GCA002090215_00902" }, { "type": "cds", "sequence": "contig_1", "start": 505376, "stop": 505873, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGAAGTGTGGAACATTGTCTTTAGTCAGTTTAACAATGATGGCAATGGTAACTACAGTGAGTTAGCGCAGAAGAACATCGATACTGGGGCCGGTATTGAACGCCTAGTAGCAATCTTACAGGACGCGCCGACTAACTTTGACACGGATATTTTCTTAAAGTTAATTGGCATTATTGAACAGCACTGTAAGCATAAGTATGATACCAACTTATACTTTAAGTTTGACCAAAAGTTAAACGAAGCGCAAAGTGCCTTTCGCATTATTAGTGACCACTTTAAGGCAATTACCTTCACCATTGCCGAAGGCGTATTGCCAGGACCCAACGAGCGCAGTTACATTGTGCGCCGGTTACTGCGCCGCGCTTTGTTGGCCTGCAAAAAACTGGACTTAGACTTAAAGTTTATTGATCCGATGGTCGATGCCATTATTAGCGTTTATGGGTCTTACTACCAGCAGTTGCAGGGGAAAAACCAGGTGGTGCAACAAGCAATCTGA", "aa": "MEVWNIVFSQFNNDGNGNYSELAQKNIDTGAGIERLVAILQDAPTNFDTDIFLKLIGIIEQHCKHKYDTNLYFKFDQKLNEAQSAFRIISDHFKAITFTIAEGVLPGPNERSYIVRRLLRRALLACKKLDLDLKFIDPMVDAIISVYGSYYQQLQGKNQVVQQAI", "aa_hexdigest": "b6913b4e3ad5fad4030215ddddc32e9e", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18876.393899999977, "isoelectric_point": 6.283743858337403 }, "id": "APKECPOBEN_919", "locus": "GCA002090215_00903" }, { "type": "cds", "sequence": "contig_1", "start": 506054, "stop": 506200, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGGGTAACTTCTCGATTGGTGATTACTTTAAGGAAGAGGCCATTGGCTTTGCCAATGATCTTTTAGTCAATCATTACCACTTGGATCCGAAGCGCTTTTACATTACAGTGTACCAAGATGATGAATTGACCTTTAACACCTGA", "aa": "MLGNFSIGDYFKEEAIGFANDLLVNHYHLDPKRFYITVYQDDELTFNT", "aa_hexdigest": "d61c1cab050927d79af6d19310353653", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5676.2373, "isoelectric_point": 4.444434165954591 }, "id": "APKECPOBEN_920", "locus": "GCA002090215_00904" }, { "type": "cds", "sequence": "contig_1", "start": 506735, "stop": 507241, "strand": "-", "frame": 3, "gene": "ugpQ", "product": "putative MG293-like protein", "db_xrefs": [ "COG:COG0584", "COG:I", "GO:0006629", "GO:0008081", "SO:0001217", "UniRef:UniRef50_P75367", "UniRef:UniRef90_P75367" ], "nt": "ATGCTTAAACGACAACTTCTGCTAGCACACCGTGGGTACAGTGATATTGCGCCTGAAAACACCCAGCTAGCGTTTGAATTAGCGTTCCAATATCGCTTTGATGGCGTGGAGTTAGATGTGCACTTGACCAAGGACGGGGAGTTAGTGATTATCCATGATGAAACTACCACCAGAACAGCGCTAGTCGACAAAACAATCGAACTGGAAACACTCGCTAGTTTAAAGCAAGATGATCACAGTGCTTTCTTTAAGTTCAAAACGCAACCCCAGCCGATTATGACCTTAAAGGAGTTCTTTGATCAGTACTTAGATAAATTTCAACTAATTAACGTGGAAATTAAAACTGACCAAAAGGAATACCCGGGAATTGAAGCTAAAATTGATGCTTTGGCCCAACAGTATGGCAAAAAAGTAATTGAAAAGGTAGTGTTCTCCTCGTTTAACTTTGCTTCCTTACAGCGCTTGTATGACATTAACCCCAACTACCAAATAGCCTTTTTGTTTTGA", "aa": "MLKRQLLLAHRGYSDIAPENTQLAFELAFQYRFDGVELDVHLTKDGELVIIHDETTTRTALVDKTIELETLASLKQDDHSAFFKFKTQPQPIMTLKEFFDQYLDKFQLINVEIKTDQKEYPGIEAKIDALAQQYGKKVIEKVVFSSFNFASLQRLYDINPNYQIAFLF", "aa_hexdigest": "9150a4b1420c7ec40b6892eac91394d1", "start_type": "ATG", "rbs_motif": null, "genes": [ "ugpQ" ], "truncated": "5-prime", "pseudogene": { "start": 506516, "stop": 507241, "observations": { "mutation": [ 506605, 506668, 506737 ], "stop": [ 506605, 506668, 506737 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75367", "query_cov": 1.0, "subject_cov": 0.6970954356846473, "identity": 1.0, "score": 330.0, "evalue": 2.23e-113, "gene_start": 1, "gene_end": 168, "reference_start": 1, "reference_end": 168, "reference_sequence": "MLKRQLLLAHRGYSDIAPENTQLAFELAFQYRFDGVELDVHLTKDGELVIIHDETTTRTALVDKTIELETLASLKQDDHSAFFKFKTQPQPIMTLKEFFDQYLDKFQLINVEIKTDQKEYPGIEAKIDALAQQYGKKVIEKVVFSSFNFASLQRLYDINPNYQIAFLFWTKKQFQAVDALKIKQVCQYLHPWTNIYEKFPDMVLSLQLPLGLWTLNSEVKFHQFRQDRMVYAQIANKKFEV" }, "paralog": false, "identity": 0.9875518672199171, "score": 468.0, "evalue": 4.56e-170, "description": "Internal stop codon at 506605, 506668, 506737. Nonsense mutation around 506605, 506668, 506737.", "psc": { "uniref90_id": "UniRef90_P75367", "gene": "UgpQ", "product": "Uncharacterized protein MG293 homolog", "uniref50_id": "UniRef50_P75367", "cog_id": "COG0584", "cog_category": "I", "go_ids": [ "GO:0008081", "GO:0006629" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75367", "UniRef:UniRef50_P75367", "COG:COG0584", "COG:I", "GO:0008081", "GO:0006629" ] }, "pscc": { "uniref50_id": "UniRef50_P75367", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75367" ], "product": "Uncharacterized protein MG293 homolog" } }, "id": "APKECPOBEN_921", "locus": "GCA002090215_00905" }, { "type": "cds", "sequence": "contig_1", "start": 507291, "stop": 507881, "strand": "-", "frame": 2, "gene": null, "product": "putative MG294-like protein", "db_xrefs": [ "GO:0022857", "KEGG:K26602", "SO:0001217", "UniRef:UniRef50_P75366", "UniRef:UniRef90_P75366" ], "nt": "ATGGGAATGTATGTCTTTCAAAGTACGTTTGCTTATTATCTTTTACAAATGATGCAAAACGCCTTTTTGGCACCAGTGATCTTAGGGACTGTACTGGGTGGTGTAAGGACGTATGTTTTGCGCAGTGCTGTAAGTGTGTACTTGGGCAGGTTAGCTGATAAGTTTAGGAGCTACATCCTCTTTTTAATGTTGTGTACAGGACTTGGCATTATCTTTGTCTTGATGTTTATTCTCTTAGGTTTTGGGCAAGTGGGCCAACAGCAAAACTATGCCTTGATTATCGTATCGGCGATCCTCTACATTCTCACCGGGGTGTTGTCTTGGGGTATGGTAACCGTTAGGTACAACCAGGTAGCGGAAATAGAGATTGGTAAAAACAACTATGCTTCTAGTGTTGGTTTACTTTCCTTTATTGGTTTTTCGACTGATGGTTGGCTCTACACGGTTACAAGTGTCGTGGGTAAGGCTTATACCCCAGACGGTCAGAAGAATACCTCGATTCAGGGTTACCAAATTATTGCAGCTATTTGTCTAGGAATAGCCCTGTTTGGTTTATTGTGTGGTACGATTGTCTTTTTGGTTAATACTTGA", "aa": "MGMYVFQSTFAYYLLQMMQNAFLAPVILGTVLGGVRTYVLRSAVSVYLGRLADKFRSYILFLMLCTGLGIIFVLMFILLGFGQVGQQQNYALIIVSAILYILTGVLSWGMVTVRYNQVAEIEIGKNNYASSVGLLSFIGFSTDGWLYTVTSVVGKAYTPDGQKNTSIQGYQIIAAICLGIALFGLLCGTIVFLVNT", "aa_hexdigest": "d292932f2a7c8b84e976e4ff46349281", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 507234, "stop": 508181, "observations": { "mutation": [ 507293, 507902 ], "stop": [ 507293, 507902 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75366", "query_cov": 1.0, "subject_cov": 0.4126315789473684, "identity": 1.0, "score": 367.0, "evalue": 3.04e-124, "gene_start": 1, "gene_end": 196, "reference_start": 261, "reference_end": 456, "reference_sequence": "METKLSLKKRLQQFSKKQLIALIILGAADVFVIAAPYYIKNVVPNLHLYLGITEDEVATLTSIIGYVTLATQLPGGFLANRFSSRKLLFLSEISTGVITFWLATNILTRESQKSNALFVQYCVIWGLWGITSTLIFWTPLWKLASQQATQENQALGFGIQGAANGVWGFIFIFLIALIITAVAYPAGGESSANNPAPFAIYAFIIGGMLLVTGFTVLFFVPEKPIEKYDSHTSLKTAKKNFEQILITLKNWKLWLLSFFLMGMYVFQSTFAYYLLQMMQNAFLAPVILGTVLGGVRTYVLRSAVSVYLGRLADKFRSYILFLMLCTGLGIIFVLMFILLGFGQVGQQQNYALIIVSAILYILTGVLSWGMVTVRYNQVAEIEIGKNNYASSVGLLSFIGFSTDGWLYTVTSVVGKAYTPDGQKNTSIQGYQIIAAICLGIALFGLLCGTIVFLVNTWELKRLGKTDYRWRTLDNA" }, "paralog": false, "identity": 0.9936507936507937, "score": 615.0, "evalue": 7.02e-224, "description": "Internal stop codon at 507293, 507902. Nonsense mutation around 507293, 507902.", "psc": { "uniref90_id": "UniRef90_P75366", "product": "Uncharacterized protein MG294 homolog", "uniref50_id": "UniRef50_P75366", "kegg_orthology_id": "K26602", "go_ids": [ "GO:0022857" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75366", "UniRef:UniRef50_P75366", "KEGG:K26602", "GO:0022857" ] }, "pscc": { "uniref50_id": "UniRef50_P75366", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75366" ], "product": "Uncharacterized protein MG294 homolog" } }, "id": "APKECPOBEN_922", "locus": "GCA002090215_00906" }, { "type": "cds", "sequence": "contig_1", "start": 507900, "stop": 508199, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGGTTTTGGTATTCAGGGCGCTGCTAATGGTGTTTGGGGTTTTATTTTTATTTTCTTAATTGCCTTGATTATTACAGCAGTAGCTTACCCAGCCGGTGGTGAAAGTAGTGCTAATAATCCCGCACCGTTTGCCATTTATGCCTTTATTATTGGTGGCATGTTGCTCGTTACTGGGTTTACCGTGCTGTTCTTTGTACCAGAAAAACCAATTGAAAAGTACGACAGTCATACCAGTTTAAAAACTGCCAAAAAGAACTTTGAACAGATTCTCATTACCTTAAAGAACTGGAAGTTGTGA", "aa": "MGFGIQGAANGVWGFIFIFLIALIITAVAYPAGGESSANNPAPFAIYAFIIGGMLLVTGFTVLFFVPEKPIEKYDSHTSLKTAKKNFEQILITLKNWKL", "aa_hexdigest": "63a585f44eef0f019808b1faa2201401", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10771.587999999987, "isoelectric_point": 9.050474739074708 }, "id": "APKECPOBEN_923", "locus": "GCA002090215_00907" }, { "type": "cds", "sequence": "contig_1", "start": 508251, "stop": 508661, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAACGAAACTTAGTTTAAAGAAACGGTTGCAGCAGTTTTCCAAAAAGCAGCTCATAGCACTAATTATTCTCGGTGCGGCTGATGTTTTTGTAATTGCGGCCCCGTACTATATTAAGAATGTGGTGCCTAACTTGCACCTGTATTTAGGGATTACTGAAGATGAAGTGGCCACCTTAACCTCGATTATTGGTTATGTGACCTTAGCCACGCAGTTACCTGGTGGTTTTTTAGCGAACCGGTTTAGTTCGCGGAAACTATTGTTCTTGTCAGAAATTTCAACGGGTGTAATTACCTTTTGGCTAGCTACTAACATTCTTACTCGTGAAAGCCAAAAATCCAATGCCTTGTTTGTACAGTACTGTGTTATTTGGGGGCTTTGGGGTATTACTTCGACCTTAATCTTTTGA", "aa": "METKLSLKKRLQQFSKKQLIALIILGAADVFVIAAPYYIKNVVPNLHLYLGITEDEVATLTSIIGYVTLATQLPGGFLANRFSSRKLLFLSEISTGVITFWLATNILTRESQKSNALFVQYCVIWGLWGITSTLIF", "aa_hexdigest": "1e1de476bf12f7f72e44b0cc1490fdea", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15201.783599999988, "isoelectric_point": 9.518063926696776 }, "id": "APKECPOBEN_924", "locus": "GCA002090215_00908" }, { "type": "cds", "sequence": "contig_1", "start": 508792, "stop": 509073, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF20258.4" ], "nt": "TTGTATGTCTCGAGTGACCCAGAAGATTTACAGCGTCGTGGCATTACCCTTTCCCACTTTAACTGGTTGTACCAACCCAATCCATTGACCCAAACCGTTCTGGTACGGATTCGCCATGCCCAACCGTTAGTGCAAGGGCACATAACAGTACAACCAAACAACGTCGTGCAAGTACAACTAGATCAACCAATTGACCGCGTTACCAACGGTCAGTATGGTGTTTTGTATACCCAGAATGGCATTTGTTTGGGGAGTGGTATCATTACCGCTAGCCAAATCTAA", "aa": "MYVSSDPEDLQRRGITLSHFNWLYQPNPLTQTVLVRIRHAQPLVQGHITVQPNNVVQVQLDQPIDRVTNGQYGVLYTQNGICLGSGIITASQI", "aa_hexdigest": "dce2dc16aa520893f5e25662d3119ee3", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF20258.4", "name": "tRNA_Me_trans_C", "length": 77, "aa_cov": 0.8172043010752689, "hmm_cov": 0.987012987012987, "evalue": 2.5319142417846296e-25, "score": 84.11976623535156, "start": 13, "stop": 88 } ], "seq_stats": { "molecular_weight": 10424.715799999996, "isoelectric_point": 6.807856178283691 }, "id": "APKECPOBEN_925", "locus": "GCA002090215_00909" }, { "type": "cds", "sequence": "contig_1", "start": 509203, "stop": 509577, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03054.22" ], "nt": "ATGTTGTGTAACCGTTTAATTAAATTTGGGCTAATGCGTGATTACTGCAAGCAGTTAGATCCCAACAGTGACTTTGCTACAGGGCATTATGCGGCACTTAGCTGGGACAACAACCAACCTTTACTGGCCATTCCCAAAGACAAGCACAAGGACCAAACCTATTTTTTAGCGCATGTCAAACCAGCGCAACTACAAGACGTGGTGTTTCCCTTGGCGCATTTACTGAAAACGGAGGTGCGGCAAATAGCGTTAGCACACCACTTTAGTGTGGCTACTAAAAAGGACTCAACCGGCATTTGCTTTATTGGGGAACGCCACTTTAGTGACTTCTTAAAGAATTATTTACCAGTCAAACCGGGGGTTATTTATGATTGA", "aa": "MLCNRLIKFGLMRDYCKQLDPNSDFATGHYAALSWDNNQPLLAIPKDKHKDQTYFLAHVKPAQLQDVVFPLAHLLKTEVRQIALAHHFSVATKKDSTGICFIGERHFSDFLKNYLPVKPGVIYD", "aa_hexdigest": "ba9d228296723ef3e820f3960e70ff9b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03054.22", "name": "tRNA_Me_trans", "length": 106, "aa_cov": 0.8387096774193549, "hmm_cov": 0.9905660377358491, "evalue": 1.4969316910614325e-38, "score": 127.94937133789062, "start": 1, "stop": 104 } ], "seq_stats": { "molecular_weight": 14199.280199999983, "isoelectric_point": 8.776741981506348 }, "id": "APKECPOBEN_926", "locus": "GCA002090215_00910" }, { "type": "cds", "sequence": "contig_1", "start": 509638, "stop": 509904, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02540.23", "PFAM:PF03054.22" ], "nt": "ATGCAAAATAAAACCGTTTTTGTCGGAATATCAGGTGGAGTGGACTCAGCTGTGAGTGCGCTCCTTTTAAAGCAACAGTACCGCGAAGTGATTGGCATTTTTATGGAGTGCTGGGACAATACACTAAATAACGACCAGTTAGGTCACCGCGCCTTTAACGAGCATAAGTCGGGTTGTTCGTCCAAAGAAGATTTTCGCGAAGCGCAAGCAATTGCCCAATTACTAGGAATCAAACTAATTAAGCAAAACCTGGTGGAACCGTACTGA", "aa": "MQNKTVFVGISGGVDSAVSALLLKQQYREVIGIFMECWDNTLNNDQLGHRAFNEHKSGCSSKEDFREAQAIAQLLGIKLIKQNLVEPY", "aa_hexdigest": "1932614864dae796562766c5f9c6253f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02540.23", "name": "NAD_synthase", "length": 82, "aa_cov": 0.9318181818181818, "hmm_cov": 0.7804878048780488, "evalue": 6.330235428168294e-08, "score": 27.460763931274414, "start": 3, "stop": 84 }, { "id": "PF03054.22", "name": "tRNA_Me_trans", "length": 85, "aa_cov": 0.9659090909090909, "hmm_cov": 0.8941176470588236, "evalue": 1.9948115373448594e-22, "score": 75.30052185058594, "start": 4, "stop": 88 } ], "seq_stats": { "molecular_weight": 9856.130999999994, "isoelectric_point": 6.052977561950684 }, "id": "APKECPOBEN_927", "locus": "GCA002090215_00911" }, { "type": "cds", "sequence": "contig_1", "start": 509905, "stop": 510294, "strand": "-", "frame": 1, "gene": null, "product": "putative MG296-like protein", "db_xrefs": [ "RefSeq:WP_010874779.1", "SO:0001217", "UniParc:UPI00001395B3", "UniRef:UniRef100_P75364", "UniRef:UniRef50_P75364", "UniRef:UniRef90_P75364" ], "nt": "ATGAAACCACAACTTTTAGCCTTGAAGCAGTTTGTGCAAACTGAGTTTGAAAAAGTTGATTTTGAGACCTTTCGCCAAAACTTTAATCGTTGTTTGGAACGCGAACAGAGTACCCTGTTAATTTATGAAGATGATGATTATGATGATCAGTCCTTCTTTTTAAAACCGATGCTCAGTGACGCCTTTTTTATTAGTAGTGAAGTGGTCAAACAACTGGATTTGTTAGCTGTTTTAGTGGACAATCCTAAAGGTGATGTCAAAAGTTGTTGTCAGAGCTTTTATGAAGCGTTAACCTTATTTATTTCAGCGCTGGCCATAACTAAGGGTGTTGATGTCGGGCGTTACCACCAGCAGTTGGGTAAGCGCTTTGGTGTTTTAACAGTTTACTAG", "aa": "MKPQLLALKQFVQTEFEKVDFETFRQNFNRCLEREQSTLLIYEDDDYDDQSFFLKPMLSDAFFISSEVVKQLDLLAVLVDNPKGDVKSCCQSFYEALTLFISALAITKGVDVGRYHQQLGKRFGVLTVY", "aa_hexdigest": "bd500a8f610e597845d6216cb1c1a641", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001395B3", "ncbi_nrp_id": "WP_010874779.1", "uniref100_id": "UniRef100_P75364", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001395B3", "RefSeq:WP_010874779.1", "UniRef:UniRef100_P75364" ] }, "ips": { "uniref100_id": "UniRef100_P75364", "uniref90_id": "UniRef90_P75364" }, "psc": { "uniref90_id": "UniRef90_P75364", "product": "Uncharacterized protein MG296 homolog", "uniref50_id": "UniRef50_P75364" }, "pscc": { "uniref50_id": "UniRef50_P75364", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75364" ], "product": "Uncharacterized protein MG296 homolog" }, "genes": [], "id": "APKECPOBEN_928", "locus": "GCA002090215_00912" }, { "type": "cds", "sequence": "contig_1", "start": 510294, "stop": 510602, "strand": "-", "frame": 2, "gene": "ylxM", "product": "YlxM family DNA-binding protein", "db_xrefs": [ "COG:COG2739", "COG:K", "GO:0048500", "KEGG:K09787", "RefSeq:WP_010874780.1", "SO:0001217", "UniParc:UPI000013A7F1", "UniRef:UniRef100_P75363", "UniRef:UniRef50_P75363", "UniRef:UniRef90_P75363" ], "nt": "ATGAACCAAACCAAACTTAACCAGCGTTTAAAGCAACAGCAACTATTTGATATTTACGGGGAACTTTTAACCAAACGGCAAGCGTGTTACTTTAACGAGTACATTAACCTGGATTTGAGCATGCAAGAGATTGCTGATAAATACCAAGTTAAGAAAAGTTCAATCCACCAACACATCAAAACCTGCAACCTGATCTTTAACCGTTTTGAAGCGAAATTGCAGTTGTTTAAAAAGCAACAGCTCCGTTTAAAGTTGTACGAAAAAATAACAGATCCCCAATTACGCGAGCAACTTATTAAACTAAGGTAA", "aa": "MNQTKLNQRLKQQQLFDIYGELLTKRQACYFNEYINLDLSMQEIADKYQVKKSSIHQHIKTCNLIFNRFEAKLQLFKKQQLRLKLYEKITDPQLREQLIKLR", "aa_hexdigest": "65aa099fec66c2c2366037bd6aea5dbc", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013A7F1", "ncbi_nrp_id": "WP_010874780.1", "uniref100_id": "UniRef100_P75363", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013A7F1", "RefSeq:WP_010874780.1", "UniRef:UniRef100_P75363" ] }, "ips": { "uniref100_id": "UniRef100_P75363", "uniref90_id": "UniRef90_P75363" }, "psc": { "uniref90_id": "UniRef90_P75363", "gene": "ylxM", "product": "YlxM family DNA-binding protein", "uniref50_id": "UniRef50_P75363", "cog_id": "COG2739", "cog_category": "K", "kegg_orthology_id": "K09787", "go_ids": [ "GO:0048500" ] }, "pscc": { "uniref50_id": "UniRef50_P75363", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75363" ], "product": "UPF0122 protein MPN_424" }, "genes": [ "ylxM" ], "id": "APKECPOBEN_929", "locus": "GCA002090215_00913" }, { "type": "cds", "sequence": "contig_1", "start": 510583, "stop": 511014, "strand": "-", "frame": 1, "gene": null, "product": "Cell division protein FTSY", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PYR8" ], "nt": "GTGATCTTCCGTGGGGTGCAACAAGGCATTCAAAACGAATACGACTTTGTGTTGTGTGACACCTCGGGACGGTTGCAAAACAAAACCAACTTAATGAATGAGTTGAAAAAGATTTACCAAATTGTCCAGAAGGTGAGTAGCGCTAAACCCCAAGAAACACTGCTCGTTTTAGATGGTACTACCGGACAATCGGGGTTAGCACAGGCCAAGGTCTTTAATGAATTTACCGAGCTTACCGGGATTATCCTTACCAAAATGGACAGCTCCTCTAAGGGTGGGATTATCCTAGCGATTAAAGACCTGTTTAACTTACCGGTAAAACTGATTGGTTTTGGAGAAACAACTGCTGATTTAGCGGCCTTTGATTTAGAGCAATATGTACTGGGTCTTACCAAGAATTTAAGCCTCAACCATGAACCAAACCAAACTTAA", "aa": "MIFRGVQQGIQNEYDFVLCDTSGRLQNKTNLMNELKKIYQIVQKVSSAKPQETLLVLDGTTGQSGLAQAKVFNEFTELTGIILTKMDSSSKGGIILAIKDLFNLPVKLIGFGETTADLAAFDLEQYVLGLTKNLSLNHEPNQT", "aa_hexdigest": "7ec3940ce35358f300c5d2fe3e475f24", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0PYR8", "query_cov": 0.8601398601398601, "subject_cov": 0.968503937007874, "identity": 0.675, "score": 169.0, "evalue": 1.15e-51, "valid": false, "product": "Cell division protein FTSY", "uniref50_id": "UniRef50_A0A3B0PYR8" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0PYR8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PYR8" ], "product": "Cell division protein FTSY" }, "genes": [], "id": "APKECPOBEN_930", "locus": "GCA002090215_00914" }, { "type": "cds", "sequence": "contig_1", "start": 511072, "stop": 511629, "strand": "-", "frame": 1, "gene": "ftsY", "product": "signal recognition particle-docking protein FtsY", "db_xrefs": [ "COG:COG0552", "COG:U", "EC:3.6.5.4", "GO:0003924", "GO:0005047", "GO:0005525", "GO:0005737", "GO:0005886", "GO:0006614", "GO:0009306", "GO:0016887", "KEGG:K03110", "SO:0001217", "UniRef:UniRef50_P75362", "UniRef:UniRef90_P75362" ], "nt": "ATGAGTTTTATCCACAAGCTAATCCAGAAGTTTAAACCGAAAAAGAAACTGGTCGACCAAGTGCAACAAGCTGTCCAGGAAAAGAGCTTCTTTCAAGCCAACCAAAAGAGCTATTACCAGGGTTTAAACAAGTCGGCTAACAGCTTTGCCAATACCATTAATAAACTAGCAGCTAACTATGTAACGGTTAACGAACAGTTTCAAGAAAGTTTGTTTGAGGAGTTAGTGTTGCTTGATATTGGTTACCATGCGGCTACTAAAATCTGTGATGCGATTGTCCAAGAACTCAAACTACAACGAGTGAGTGATCCTCAGTTAATCCAGGAAATTATTGTCGATAAGTTAATTGTTTACTATATCCAAGATAAGTTGTTTGAAACTGATTTAACGGTTGAAGCTAATAAAACCAACGTGTATTTGTTTGTCGGGGTTAATGGTGTTGGCAAAACAACTTCATTAGCCAAACTAGCTGACCAGCTTACCAAGCAAAACAAACGGGTGTTAATGGTCGCTGGGGATACCTTTCGCGCTGGGGCAGTGGCCCAGTTAGCAGAATGA", "aa": "MSFIHKLIQKFKPKKKLVDQVQQAVQEKSFFQANQKSYYQGLNKSANSFANTINKLAANYVTVNEQFQESLFEELVLLDIGYHAATKICDAIVQELKLQRVSDPQLIQEIIVDKLIVYYIQDKLFETDLTVEANKTNVYLFVGVNGVGKTTSLAKLADQLTKQNKRVLMVAGDTFRAGAVAQLAE", "aa_hexdigest": "72e09c2c86b35f85ac3ac3bab2b294cf", "start_type": "ATG", "rbs_motif": null, "genes": [ "ftsY" ], "truncated": "5-prime", "pseudogene": { "start": 510769, "stop": 511629, "observations": { "mutation": [ 511074 ], "stop": [ 511074 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75362", "query_cov": 1.0, "subject_cov": 0.5316091954022989, "identity": 1.0, "score": 345.0, "evalue": 2.83e-117, "gene_start": 1, "gene_end": 185, "reference_start": 1, "reference_end": 185, "reference_sequence": "MSFIHKLIQKFKPKKKLVDQVQQAVQEKSFFQANQKSYYQGLNKSANSFANTINKLAANYVTVNEQFQESLFEELVLLDIGYHAATKICDAIVQELKLQRVSDPQLIQEIIVDKLIVYYIQDKLFETDLTVEANKTNVYLFVGVNGVGKTTSLAKLADQLTKQNKRVLMVAGDTFRAGAVAQLAEWAQRIGCDIVLPNPKEETPAVIFRGVQQGIQNEYDFVLCDTSGRLQNKTNLMNELKKIYQIVQKVSSAKPQETLLVLDGTTGQSGLAQAKVFNEFTELTGIILTKMDSSSKGGIILAIKDLFNLPVKLIGFGETTADLAAFDLEQYVLGLTKNLSLNHEPNQT" }, "paralog": false, "identity": 0.9965034965034965, "score": 551.0, "evalue": 5.81e-201, "description": "Internal stop codon at 511074. Nonsense mutation around 511074.", "psc": { "uniref90_id": "UniRef90_P75362", "gene": "ftsY", "product": "signal recognition particle-docking protein FtsY", "ec_ids": [ "3.6.5.4" ], "uniref50_id": "UniRef50_P75362", "cog_id": "COG0552", "cog_category": "U", "kegg_orthology_id": "K03110", "go_ids": [ "GO:0003924", "GO:0005047", "GO:0005525", "GO:0005737", "GO:0005886", "GO:0006614", "GO:0009306", "GO:0016887" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75362", "EC:3.6.5.4", "UniRef:UniRef50_P75362", "COG:COG0552", "COG:U", "KEGG:K03110", "GO:0003924", "GO:0005047", "GO:0005525", "GO:0005737", "GO:0005886", "GO:0006614", "GO:0009306", "GO:0016887" ] }, "pscc": { "uniref50_id": "UniRef50_P75362", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75362" ], "product": "Signal recognition particle receptor FtsY" } }, "id": "APKECPOBEN_931", "locus": "GCA002090215_00915" }, { "type": "cds", "sequence": "contig_1", "start": 511635, "stop": 514130, "strand": "-", "frame": 2, "gene": "smc", "product": "chromosome segregation protein SMC", "db_xrefs": [ "COG:COG1196", "COG:D", "GO:0003677", "GO:0005524", "GO:0005694", "GO:0005737", "GO:0006260", "GO:0007059", "GO:0007062", "GO:0016887", "GO:0030261", "KEGG:K03529", "SO:0001217", "UniRef:UniRef50_P75361", "UniRef:UniRef90_P75361" ], "nt": "GTGGAAGCCAAACCAGAGGAGCGACGTAAGATCTTTGAGGACGCTTCGGGGATTGGGCGGTACACTAAGCGCAAAGAAGAGGTTACCAACCAATTAGCGCGTACCGTGCAAAATCTCAAACAGGTCAGCATTGTTTTAAATGAACTCAAAAAGGACTTAAAGAAGCTTACCATTCAAGCGGACAAGGCCCAGCGCTTTGTCAAACTTAAGGAAGAATTAAAGGAGCTGGAGCTGAGTGTCTTGGTCGCAGATTACCTCAAGTCCCAGGGTGAGTTAGACCGCTTTAACCACCAAATTGGTTACATCGAACAGGACTTTAAACTACACGAACCGCAGTTACAACTGCTAGAAGACCAGCTCAACATCTTTAACCAGCGCTTTCGTGATGCTGATGAACAGTCCATCAAACTACAACAAGAACTGCAAGCGGTCTACCAAACGATTAACGAGTTGGAACAACGCAAGGCCGTGATTGATGTGCAGCTCAAAAACGAGTTGTCCAAAAAGGACGAAAAGCACAAAATCCAGGCGTTAAAAAAGTTAATCCGGGTAGACCAAGCGCAACTAGAAAGCTTACAAGCGCAAGTACTCAAAACAACCAGTGAAATTACCCTGTTAACCAATGAGTTGAGCACCGTCCAAACTGAGTTGGACACCACAAAGTTGAATCTTAACCAAAACAGTGCGGCGTTAGTCTACCAACAAGCGCAGCAAGAGTTTTTAAAAGCGCAAAACGAGGAGTGGGTCAAAACGAATCCGGCCCATGTTTTGGTCAAAAATGTCAAGGCCCTCACCGGTCTCTTAAACACGCTCAACACTTTTTTAAAGTTTGAAAAGCAGTACGAAAAGGCCTTGTTAAAGGCGTTGGGTAAATCAATTGGTTATCTGGTTGTTAACAACAACTTGGCGGCTTTAAAGGCAATTGATTTTTTGTTAACTAACCAGATTGGTCAAGTTACCTTTTTACCAATTGACGACATTGCCTTTGATACTAAGATAGCTCCCGAGCACATGGAAATTTTGCAACAACTCGATGGCTTTTTGGGTGTTGGTAGTGACCATGTGAGTTGTGATGAAAGCTTACAACCAATTGTTAATGCTTTATTGGGTCAAGTAATTATTGCCAGTGACCTTCAAGCCGCTTTAAAACTGTCCAGTTATACCTATAAGCTGTACCGCGTTGTCACCCTCAACGGGGAAACGGTGTACGCTGGAGGGATTATCCAGGGTGGTTATGTCAAGGACAACTTAAGCTTGTATAACCTCCAGGAAAAGTTAGCTTCTAGCGAAGCTAACATTACCCAACTAGAACATAACGAAAAGCAACTACGTACCAATCTCACTAGCTTAGAGACTAAACTCAATGAGCTCAACAAAAAGTTAAAGTACGAAGAAATCCTACTGGAAAAGTTTAACGAACGTGTTAACCACACCAATAAGGCAATTTTGAGCTATAAGATTGAGTATGAGCAGCTCACCAACGAAAGCTTTGACGGAACCCCGCACAGCTTTGATGAAACCAGATTGGTTGAATCATTAAACCGTGCTTGGGCTGAACGTGACGAACTCAATTCACAGTTAAAGCTCAACCAGGAATTGAAAGAAACACTGGCCAAATCGATTAAACTAGCCGAAGCCAAAACCGCTGACCTCCGTGCCCTTTTAGATGAACAACGCTCCCAGTTAGTGTTGGCACGCGAAGGCAAAATCCGCTTTGAAAACACAATCCACAACATTACCGACAAGATTAACGGTGGGTATAAACTAACGATGGAGTTTGCGATCGCTAACTATAACAAACCAATTAAGCTCTCCACCATGCAAGCGCAAAACAAGATTGCCAGGATGCAGAGCCAACTCGATGAAATGGGTCCAATTAACCTGGAGTCGATTGCTGAAATTGCTGATAAGCAAAAGCGCTTTGATGACATTAACGGTGAGTATGAATCACTGCAAACGGCGATTAAGGATTTGCAAACCGCGATTGGTGAAATTGATGAATTGGCCTGCAAGGAATTTGACGAGCTCATCCAAAAAGTTAATGCAGAACTACCTAAAACCTTTAACTACCTCTTTGGCGGTGGTTCGTGTCAAATTCGCTACACGGATACTGATAACGTCTTGTTGTCAGGCATAGAAGTGTTTGCTAATCCGCCTGGCAAAAATGTCGCTAACTTAATGTTGTTATCGGGTGGGGAGAAAACACTCGTGGCCTTGTCGGTCCTCTTTAGTATTTTGAGAGTTAGTGCCTTTCCTCTAGTAATTTTAGATGAAGCGGAGAGTGCCTTAGATCCGGCGAACGTGGAGCGCTTTGCTAACATTATTGGCAATTCCAGTAACAACACCCAGTTCTTAATTATTACCCACCGTCAGGGCACGATGATGAAGTGTGACATGTTACTGGGAGCGGCAATGCAGACCAAGGGAGTGACCAAGACGTTTGCCGTTTCTTTGGAAAAAGCAGAGCAGTACATTAGTAAAGATAAGCAGAACTAA", "aa": "MEAKPEERRKIFEDASGIGRYTKRKEEVTNQLARTVQNLKQVSIVLNELKKDLKKLTIQADKAQRFVKLKEELKELELSVLVADYLKSQGELDRFNHQIGYIEQDFKLHEPQLQLLEDQLNIFNQRFRDADEQSIKLQQELQAVYQTINELEQRKAVIDVQLKNELSKKDEKHKIQALKKLIRVDQAQLESLQAQVLKTTSEITLLTNELSTVQTELDTTKLNLNQNSAALVYQQAQQEFLKAQNEEWVKTNPAHVLVKNVKALTGLLNTLNTFLKFEKQYEKALLKALGKSIGYLVVNNNLAALKAIDFLLTNQIGQVTFLPIDDIAFDTKIAPEHMEILQQLDGFLGVGSDHVSCDESLQPIVNALLGQVIIASDLQAALKLSSYTYKLYRVVTLNGETVYAGGIIQGGYVKDNLSLYNLQEKLASSEANITQLEHNEKQLRTNLTSLETKLNELNKKLKYEEILLEKFNERVNHTNKAILSYKIEYEQLTNESFDGTPHSFDETRLVESLNRAWAERDELNSQLKLNQELKETLAKSIKLAEAKTADLRALLDEQRSQLVLAREGKIRFENTIHNITDKINGGYKLTMEFAIANYNKPIKLSTMQAQNKIARMQSQLDEMGPINLESIAEIADKQKRFDDINGEYESLQTAIKDLQTAIGEIDELACKEFDELIQKVNAELPKTFNYLFGGGSCQIRYTDTDNVLLSGIEVFANPPGKNVANLMLLSGGEKTLVALSVLFSILRVSAFPLVILDEAESALDPANVERFANIIGNSSNNTQFLIITHRQGTMMKCDMLLGAAMQTKGVTKTFAVSLEKAEQYISKDKQN", "aa_hexdigest": "51676cb8ac5dc3badf0230d04823fec4", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75361", "query_cov": 1.0, "subject_cov": 0.8462321792260692, "identity": 0.9990000000000001, "score": 1436.0, "evalue": 0.0, "valid": true, "gene": "smc", "product": "chromosome segregation protein SMC", "uniref50_id": "UniRef50_P75361", "cog_id": "COG1196", "cog_category": "D", "kegg_orthology_id": "K03529", "go_ids": [ "GO:0003677", "GO:0005524", "GO:0005694", "GO:0005737", "GO:0006260", "GO:0007059", "GO:0007062", "GO:0016887", "GO:0030261" ] }, "pscc": { "uniref50_id": "UniRef50_P75361", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75361" ], "product": "Chromosome partition protein Smc" }, "genes": [ "smc" ], "id": "APKECPOBEN_932", "locus": "GCA002090215_00916" }, { "type": "cds", "sequence": "contig_1", "start": 514134, "stop": 514583, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13175.12", "PFAM:PF13304.12", "PFAM:PF13476.12", "PFAM:PF13555.12", "PFAM:PF02463.25" ], "nt": "ATGGTTTTTCTCAAACGGTTTCGTGCGTATGGTTTTAAGTCATATGCTGACGAAATAACAATTAATTTTACCCACTCAATGACCGGTATAGTCGGTCCTAATGGTTCTGGCAAGTCCAACGTTGTCGATGCTTTGAAGTGGGTCTTAGGGGAACGCAGCATGAAGCACTTGCGCAGTAAGTCCGGTGATGATATGATCTTCTTTGGTTCTAAGGACAAGCCCGCTAGTAAGCTAGCGGAAGTGGAACTGACCTTTGACAATTCCCAAAAACTACTGCACGATCCCCGACCAGAGATCTCGGTGATGCGCCGGATCTATCGTGGTAGTGGTCAGAGTGAGTACTATATTAACGGCGAGTTAGTAACGCTAAAAGAAATTAGTGGGATCTTTGCTGATATTGGTTTGGAAAAAGGCTCGTTGGGGATTATTTCCCAGGGCAGTGTGTCGTGA", "aa": "MVFLKRFRAYGFKSYADEITINFTHSMTGIVGPNGSGKSNVVDALKWVLGERSMKHLRSKSGDDMIFFGSKDKPASKLAEVELTFDNSQKLLHDPRPEISVMRRIYRGSGQSEYYINGELVTLKEISGIFADIGLEKGSLGIISQGSVS", "aa_hexdigest": "a809eed79516bc538aaff6562854ba4f", "start_type": "ATG", "rbs_motif": "AAAA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13175.12", "name": "AAA_15", "length": 65, "aa_cov": 0.4228187919463087, "hmm_cov": 0.7692307692307693, "evalue": 1.0112592944425012e-10, "score": 37.22410202026367, "start": 11, "stop": 73 }, { "id": "PF13304.12", "name": "AAA_21", "length": 67, "aa_cov": 0.3221476510067114, "hmm_cov": 1.0, "evalue": 1.964513379794516e-09, "score": 33.38786315917969, "start": 27, "stop": 74 }, { "id": "PF13476.12", "name": "AAA_23", "length": 124, "aa_cov": 0.8053691275167785, "hmm_cov": 0.9354838709677419, "evalue": 6.6526696627703486e-18, "score": 61.50141525268555, "start": 11, "stop": 130 }, { "id": "PF13555.12", "name": "AAA_29", "length": 37, "aa_cov": 0.24161073825503357, "hmm_cov": 0.972972972972973, "evalue": 4.069927152790179e-08, "score": 28.39857292175293, "start": 14, "stop": 49 }, { "id": "PF02463.25", "name": "SMC_N", "length": 137, "aa_cov": 0.912751677852349, "hmm_cov": 0.9854014598540146, "evalue": 7.976612501193685e-43, "score": 141.84580993652344, "start": 3, "stop": 138 } ], "seq_stats": { "molecular_weight": 16498.679799999987, "isoelectric_point": 9.124742317199708 }, "id": "APKECPOBEN_933", "locus": "GCA002090215_00917" }, { "type": "cds", "sequence": "contig_1", "start": 514768, "stop": 515640, "strand": "+", "frame": 1, "gene": "cof", "product": "Putative phosphatase MPN_427", "db_xrefs": [ "COG:COG0561", "COG:HR", "EC:3.1.3.-", "GO:0000287", "GO:0005829", "GO:0016791", "RefSeq:WP_010874783.1", "SO:0001217", "UniParc:UPI000013AABA", "UniRef:UniRef100_P75360", "UniRef:UniRef50_P75360", "UniRef:UniRef90_P75360" ], "nt": "ATGACAAAGACAAGTAAAGCATCCGGGTTAAGCTGGTTTTTTTGTGATTTAGATGGCACTTTACTGCGTTACCAAAACAACCAGCACTTAATTGAACCAACTACGAAGCGAGCAGTGGCACAGTTAGTGGAGAGTGGTGCCAACTTTGTTGTAGCTACTGGACGCAAACCGAGTGACGTGCGCAACATTTACAAGGAGTTGGGGATTGAACAAGCCAGTCCCTACTTAATTGCCAACAACGGCGCGGTAGTGTGGGATTTAAAGCGCAATAGCTACCTCAACAAGCAAACCTTGTCTTTAAGTGACTTTGACTTAATTGACCACATTAACCAAACGTTAAACCAATTAAACCACGAGTATGGCTGCATTCTGTATGGCCTAAACGACCAGGTGTACTTTTATCATATCCACGCTCCTGACTCACAGGCCTTCAAACAGTACTTTGCTTTTTATGAAGGCGAATTTGTCCAAAACCAGTATCTGGAAATAGATGGGCTCAAAACTGAATACAATCTAGTTAAAGCCATTTGGTTCTTTAAGGAAGTGCACCAGCAAAAGGCGGTGATTGCGCAACACTTTACTAACCAAGAACGCTTGGTCATTACTTCAGCACACTCGTTTGAACTAGTACCGCTTAATGTATCAAAGGGTCATGCGATAAACCTAATTAAACAACAAGTAAAAATCACCGATAACCAGATTATGGTCCTTGGTGACTCTTACAATGACTTGCCAATGTTTCAACATGGTGTAGTTAAGGTAACTAATCACCTCGCACCCGACAACCTCAAACAACTAGCTACAAGGGTTTATGAACTACCAGCTTCACTGTTTGTGGGTCAAGCGTTGAACGACTACTTTAAGTTCGATTAA", "aa": "MTKTSKASGLSWFFCDLDGTLLRYQNNQHLIEPTTKRAVAQLVESGANFVVATGRKPSDVRNIYKELGIEQASPYLIANNGAVVWDLKRNSYLNKQTLSLSDFDLIDHINQTLNQLNHEYGCILYGLNDQVYFYHIHAPDSQAFKQYFAFYEGEFVQNQYLEIDGLKTEYNLVKAIWFFKEVHQQKAVIAQHFTNQERLVITSAHSFELVPLNVSKGHAINLIKQQVKITDNQIMVLGDSYNDLPMFQHGVVKVTNHLAPDNLKQLATRVYELPASLFVGQALNDYFKFD", "aa_hexdigest": "e0b7a6304a261db452402cc6b69a533b", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013AABA", "ncbi_nrp_id": "WP_010874783.1", "uniref100_id": "UniRef100_P75360", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013AABA", "RefSeq:WP_010874783.1", "UniRef:UniRef100_P75360" ] }, "ips": { "uniref100_id": "UniRef100_P75360", "uniref90_id": "UniRef90_P75360" }, "psc": { "uniref90_id": "UniRef90_P75360", "gene": "Cof", "product": "Putative phosphatase MPN_427", "ec_ids": [ "3.1.3.-" ], "uniref50_id": "UniRef50_P75360", "cog_id": "COG0561", "cog_category": "HR", "go_ids": [ "GO:0005829", "GO:0000287", "GO:0016791" ] }, "pscc": { "uniref50_id": "UniRef50_P75360", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75360" ], "product": "Putative phosphatase MPN_427" }, "genes": [ "cof" ], "id": "APKECPOBEN_934", "locus": "GCA002090215_00918" }, { "type": "cds", "sequence": "contig_1", "start": 515646, "stop": 516518, "strand": "-", "frame": 2, "gene": "pta", "product": "phosphate acetyltransferase", "db_xrefs": [ "COG:C", "COG:COG0280", "EC:2.3.1.8", "GO:0005737", "GO:0006085", "GO:0006086", "GO:0008959", "SO:0001217", "UniRef:UniRef50_P47541", "UniRef:UniRef90_P75359" ], "nt": "ATGGAAGCAGTTAACCAGCTCCAGCAAGCGGGTATTTTAACCCCAGCGGTGATCTTCCGCACCCGCAGTGAAGTACCAGCTGGTTTTAACACCGCCATCAAGCACTATGTGATTGAGGAAATGGACTTGACAAAGTACGCTAACTTCGTTTATGAAAAGCGCAAGCACAAGGGCATGGAAATGCGTGAAGCACAAAAGTTTGTCCGTGATGCCAGTTCCCTAGCAGCCACTTTAGTAGCACTCAACGAAGTGGATGGGGAAGTGTGTGGTAAGGAATACGCTACGAAGGATACTCTCCGTCCTGCTTTACAACTGTTAGGTACTGGTAACTTTGTGTCGAGTGTGTTCATCATGGAAAAGAATGAGGAACGCTTGTACTTTACCGACTGTGCTTTTGCTGTTTACCCTTCACCCCAAGAATTAGCAGTTGTAGCGGAAAACACCTTTAAGTTTGCCCAAAGTATGGGGGAACCAGAACTCAAGATGGTCTTTTTGAGCTATTCAACACTGGGCAGTGGTAAAGGGGAAGCGGTGGACAAAGTAGTCAGTGCGACCCAAATCTTCTTGGAAAAACACCCCGAACTCAAAGCTAATGTTTGTGGTGAATTACAGTTTGACAGTGCTTTTGTGGAAAAGGTACGCAAACAAAAAGCCCCTAATTTGACTTGGAATGGATCAGCTAACATCTATGTGTTCCCGAACCTAGATGCTGGTAACATTGGATACAAGATTGCGCAACGTTTAGGTGGTTATGAAGCAATTGGTCCAATTGTTTTAGGTCTAGCACGTCCTTTTAACGACCTTTCCCGTGGTGCCAGTGTCAGTGATGTCTTTAACGTGGGCATTATTACCGCAGCGCAAACACTTAAATAA", "aa": "MEAVNQLQQAGILTPAVIFRTRSEVPAGFNTAIKHYVIEEMDLTKYANFVYEKRKHKGMEMREAQKFVRDASSLAATLVALNEVDGEVCGKEYATKDTLRPALQLLGTGNFVSSVFIMEKNEERLYFTDCAFAVYPSPQELAVVAENTFKFAQSMGEPELKMVFLSYSTLGSGKGEAVDKVVSATQIFLEKHPELKANVCGELQFDSAFVEKVRKQKAPNLTWNGSANIYVFPNLDAGNIGYKIAQRLGGYEAIGPIVLGLARPFNDLSRGASVSDVFNVGIITAAQTLK", "aa_hexdigest": "151464f33bb12b05af6bc4ab739144ff", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75359", "query_cov": 1.0, "subject_cov": 0.90625, "identity": 0.997, "score": 570.0, "evalue": 9.08e-205, "valid": true, "gene": "pta", "product": "phosphate acetyltransferase", "ec_ids": [ "2.3.1.8" ], "uniref50_id": "UniRef50_P47541", "cog_id": "COG0280", "cog_category": "C", "go_ids": [ "GO:0005737", "GO:0006085", "GO:0006086", "GO:0008959" ] }, "pscc": { "uniref50_id": "UniRef50_P47541", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47541" ], "product": "Phosphate acetyltransferase" }, "genes": [ "pta" ], "id": "APKECPOBEN_935", "locus": "GCA002090215_00919" }, { "type": "cds", "sequence": "contig_1", "start": 516646, "stop": 516960, "strand": "-", "frame": 1, "gene": null, "product": "phosphoglycerate kinase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021F8CEA1" ], "nt": "ATGTCCTTGGATGTTGGTAGCAAAACAGTGGCCCTGTTTAGCAGTTACCTCGCCAAGGCCAAAACAATCTTTTGGAATGGTCCCATGGGCGTATTTGAATTTAGCAACTATGCTCAGGGTACCTTAGCAATTGGTAAAGCGATTGCGCAAAACCAACAAGCCTTTAGTGTCATTGGCGGTGGTGATTCTGCAGCCGCTGCTAAACAACTCCAGATTGCTGATCAATTTAGTTTTATTTCCACTGGTGGTGGTGCATCTTTAGCACTAATAGGTGGGGAAGAGCTAGTGGGAATTAGCGATATTCAAAAAAAGTAA", "aa": "MSLDVGSKTVALFSSYLAKAKTIFWNGPMGVFEFSNYAQGTLAIGKAIAQNQQAFSVIGGGDSAAAAKQLQIADQFSFISTGGGASLALIGGEELVGISDIQKK", "aa_hexdigest": "583382ee057a7fee52f78e5db83ec487", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0021F8CEA1", "query_cov": 0.8269230769230769, "subject_cov": 0.8958333333333334, "identity": 0.605, "score": 105.0, "evalue": 8.99e-28, "valid": false, "product": "phosphoglycerate kinase", "ec_ids": [ "2.7.2.3" ], "uniref50_id": "UniRef50_UPI0021F8CEA1", "kegg_orthology_id": "K00927" }, "pscc": { "uniref50_id": "UniRef50_UPI0021F8CEA1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021F8CEA1" ], "product": "phosphoglycerate kinase" }, "genes": [], "id": "APKECPOBEN_936", "locus": "GCA002090215_00920" }, { "type": "cds", "sequence": "contig_1", "start": 516982, "stop": 517428, "strand": "-", "frame": 1, "gene": null, "product": "phosphoglycerate kinase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000851E545" ], "nt": "TTGGGTGACATCTTTGTCAATGACGCCTTTGGCACCGCCCACCGCCGTCACGCTTCCAACGCTGGCATTGCCAAGTACATCAAAAACTCCTGCATTGGTCTTTTAATGGAGCGGGAATTGATCAACCTCTACCGCTTAATTAATAACCCACCCAAACCGTTTGTGGTTGTGTTGGGTGGCGCTAAGGTTTCTGATAAGTTACAGGTAGTAAATAACCTGTTCAAGATTGCGGATCATATTTTAATAGGTGGGGGCATGGTCAACACCTTCCTCAAAGCTCTGGGTAACGAAGTTGGCACTTCCTTAGTGGAGGAAGACTTAGTCCAAACCGCCAAGCAAATCCTTGACAGTGACAAGGACAAGAAAATTGTCTTGGGGGTAGACCAAATGCTTCATGCTAGCTTTAAGGATGAACCAGGCGTGGAGTGTGTTGTAGCCCCCCAATGA", "aa": "MGDIFVNDAFGTAHRRHASNAGIAKYIKNSCIGLLMERELINLYRLINNPPKPFVVVLGGAKVSDKLQVVNNLFKIADHILIGGGMVNTFLKALGNEVGTSLVEEDLVQTAKQILDSDKDKKIVLGVDQMLHASFKDEPGVECVVAPQ", "aa_hexdigest": "2d6cbbf89a091422cc072b42f753ffdc", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00210B3D86", "query_cov": 0.9054054054054054, "subject_cov": 1.0, "identity": 0.5, "score": 132.0, "evalue": 3.33e-37, "valid": false, "product": "phosphoglycerate kinase", "ec_ids": [ "2.7.2.3" ], "uniref50_id": "UniRef50_UPI000851E545", "kegg_orthology_id": "K00927" }, "pscc": { "uniref50_id": "UniRef50_UPI000851E545", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000851E545" ], "product": "phosphoglycerate kinase" }, "genes": [], "id": "APKECPOBEN_937", "locus": "GCA002090215_00921" }, { "type": "cds", "sequence": "contig_1", "start": 517435, "stop": 517875, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00162.24" ], "nt": "ATGGTTGACTTTAAAACAGTCCAAGCGTTTGACTTTCAGGGTAAAACCGTAGTCTTACGTGCTGACCTCAATGTGCCGATGAAGGACGGGGTCATTACCGACAACGAACGCATACTAGCTAGCTTAGACACGATTAAGTACCTCTTGGGTCACAATTGCAAAATTGTGTTACTGTCCCATTTGTCACGAGTCAAATCGCTCGATGACAAAAAGGGTAAGAAGTCACTCCAACCAGTAGCTAGTGCTTTACAAAATTTATTAAAGAACACCAAGGTTCACTTTTGTCCGGAAAACACGGGTGACAAGGTCAAAGTCGCAGTCAATCAACTCCCATTAGGGGAAATTTTGGTGTTGGAAAACACGCGTTACTGTGATGTGAATGAGGCTGGTGAAGTGGTTAAACACGAAAGCAAGAATAACGCTGAGTTAGCACAGTTCTGA", "aa": "MVDFKTVQAFDFQGKTVVLRADLNVPMKDGVITDNERILASLDTIKYLLGHNCKIVLLSHLSRVKSLDDKKGKKSLQPVASALQNLLKNTKVHFCPENTGDKVKVAVNQLPLGEILVLENTRYCDVNEAGEVVKHESKNNAELAQF", "aa_hexdigest": "b637979097312dfb25b7c8c993060826", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00162.24", "name": "PGK", "length": 137, "aa_cov": 0.9315068493150684, "hmm_cov": 0.9051094890510949, "evalue": 1.0385853064322444e-36, "score": 122.14776611328125, "start": 8, "stop": 143 } ], "seq_stats": { "molecular_weight": 16229.596799999985, "isoelectric_point": 8.40469493865967 }, "id": "APKECPOBEN_938", "locus": "GCA002090215_00922" }, { "type": "cds", "sequence": "contig_1", "start": 517878, "stop": 518600, "strand": "-", "frame": 2, "gene": "gap", "product": "type I glyceraldehyde-3-phosphate dehydrogenase", "db_xrefs": [ "COG:COG0057", "COG:G", "EC:1.2.1.12", "GO:0004365", "GO:0005737", "GO:0006006", "GO:0006096", "GO:0016020", "GO:0016620", "GO:0031639", "GO:0034394", "GO:0050661", "GO:0051287", "SO:0001217", "UniRef:UniRef50_P47543", "UniRef:UniRef90_P75358" ], "nt": "GTGGTTGAATCCACTGGTCGCTTTGTTAGTGAAGAAGGTGCTAGTTTGCACTTGCAAGCTGGTGCTAAACGCGTGATTATTTCCGCTCCAGCTAAACAAAAAACAATTAAAACCGTGGTTTACAACGTGAACCACAAGATTATTAACGCTGAGGACAAAATTATCTCAGCTGCTAGCTGTACCACCAATTGCTTAGCACCAATGGTACACGTCTTGGAAAAGAACTTCGGCATCTTACACGGTACCATGGTAACGGTACACGCTTACACCGCCGACCAACGTTTACAAGACGCACCCCACAGCGATCTTCGTCGTGCCCGTGCTGCTGCTTGCAACATTGTACCTACCACCACTGGAGCTGCCAAAGCAATTGGCCTAGTAGTGCCAGAAGCTACTGGTAAGCTCAACGGTATGGCCCTTCGTGTTCCCGTACTAACTGGTTCGATCGTTGAACTTTGTGTAGCCCTTGAAAAGGATGCTACCGTAGAACAAATTAACCAAGCAATGAAGAAGGCGGCTTCCGCTTCCTTTAGGTACTGTGAGGATGAAATTGTGTCCAGTGACATTGTCGGCTCGGAACACGGTTCCATCTTTGACTCCAAGCTAACCAACATTATTGAAGTGGATGGTAACAAGCTTTACAAGGTTTACGCATGGTATGACAACGAATCCTCTTACGTAAACCAGTTGGTACGGGTAGTTAACTATTGTGCCAAGCTTTAA", "aa": "MVESTGRFVSEEGASLHLQAGAKRVIISAPAKQKTIKTVVYNVNHKIINAEDKIISAASCTTNCLAPMVHVLEKNFGILHGTMVTVHAYTADQRLQDAPHSDLRRARAAACNIVPTTTGAAKAIGLVVPEATGKLNGMALRVPVLTGSIVELCVALEKDATVEQINQAMKKAASASFRYCEDEIVSSDIVGSEHGSIFDSKLTNIIEVDGNKLYKVYAWYDNESSYVNQLVRVVNYCAKL", "aa_hexdigest": "49f813cf80852db2683f96dfcbf204c1", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0012BB6B15", "query_cov": 0.9958333333333333, "subject_cov": 0.9409448818897638, "identity": 0.544, "score": 230.0, "evalue": 1.84e-72, "valid": false, "gene": "gap", "product": "type I glyceraldehyde-3-phosphate dehydrogenase", "uniref50_id": "UniRef50_A0A960BYG6", "go_ids": [ "GO:0006006", "GO:0016620", "GO:0050661", "GO:0051287" ] }, "genes": [ "gap" ], "truncated": "3-prime", "pseudogene": { "start": 517878, "stop": 518891, "observations": { "mutation": [ 518624 ], "stop": [ 518624 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75358", "query_cov": 1.0, "subject_cov": 0.712166172106825, "identity": 0.996, "score": 419.0, "evalue": 7.96e-146, "gene_start": 1, "gene_end": 240, "reference_start": 98, "reference_end": 337, "reference_sequence": "MLAKSKTIRVAINGFGRIGRLVFRALLSQKNIEIVAVNDLTHPDTLAHLLKYDSAHGEFKKKVVAKDNTLMIDKKKVLVFSEKDPANLPWAEHNIDIVVESTGRFVSEEGASLHLQAGAKRVIISAPAKQKTIKTVVYNVNHKIINAEDKIISAASCTTNCLAPMVHVLEKNFGILHGTMVTVHAYTADQRLQDAPHSDLRRARAAACNIVPTTTGAAKAIGLVVPEATGKLNGMALRVPVLTGSIVELCVALEKDATVEQINQAMKKAASASFRYCEDEIVSSDIVGSEHGSIFDSKLTNIIEVDGNKLYKVYAWYDNESSYVNQLVRVVNYCAKL" }, "paralog": false, "identity": 0.9970326409495549, "score": 608.0, "evalue": 6.78e-223, "description": "Internal stop codon at 518624. Nonsense mutation around 518624.", "psc": { "uniref90_id": "UniRef90_P75358", "gene": "gap", "product": "type I glyceraldehyde-3-phosphate dehydrogenase", "ec_ids": [ "1.2.1.12" ], "uniref50_id": "UniRef50_P47543", "cog_id": "COG0057", "cog_category": "G", "go_ids": [ "GO:0004365", "GO:0005737", "GO:0006006", "GO:0006096", "GO:0016020", "GO:0016620", "GO:0031639", "GO:0034394", "GO:0050661", "GO:0051287" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75358", "EC:1.2.1.12", "UniRef:UniRef50_P47543", "COG:COG0057", "COG:G", "GO:0004365", "GO:0005737", "GO:0006006", "GO:0006096", "GO:0016020", "GO:0016620", "GO:0031639", "GO:0034394", "GO:0050661", "GO:0051287" ] }, "pscc": { "uniref50_id": "UniRef50_P47543", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47543" ], "product": "Glyceraldehyde-3-phosphate dehydrogenase" } }, "id": "APKECPOBEN_939", "locus": "GCA002090215_00923" }, { "type": "cds", "sequence": "contig_1", "start": 518622, "stop": 518891, "strand": "-", "frame": 2, "gene": null, "product": "Glyceraldehyde-3-phosphate dehydrogenase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2X0XC69" ], "nt": "ATGCTAGCAAAGAGTAAGACTATCCGTGTTGCTATCAATGGATTTGGACGTATTGGACGCCTTGTTTTTCGTGCACTTTTAAGCCAAAAAAATATTGAGATCGTTGCCGTTAACGACCTCACCCATCCCGATACTTTAGCACACTTACTAAAGTACGACTCCGCTCACGGTGAGTTTAAGAAAAAGGTAGTAGCTAAGGACAACACCTTAATGATTGACAAAAAGAAGGTGTTAGTGTTTAGTGAAAAAGATCCTGCTAACCTGCCATGA", "aa": "MLAKSKTIRVAINGFGRIGRLVFRALLSQKNIEIVAVNDLTHPDTLAHLLKYDSAHGEFKKKVVAKDNTLMIDKKKVLVFSEKDPANLP", "aa_hexdigest": "c1be61464e39cd0954b4529b83755861", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A2X0XC69", "query_cov": 0.9213483146067416, "subject_cov": 0.9425287356321839, "identity": 0.537, "score": 92.8, "evalue": 5.82e-23, "valid": false, "product": "Glyceraldehyde-3-phosphate dehydrogenase", "uniref50_id": "UniRef50_A0A2X0XC69" }, "pscc": { "uniref50_id": "UniRef50_A0A2X0XC69", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2X0XC69" ], "product": "Glyceraldehyde-3-phosphate dehydrogenase" }, "genes": [], "id": "APKECPOBEN_940", "locus": "GCA002090215_00924" }, { "type": "cds", "sequence": "contig_1", "start": 519051, "stop": 519623, "strand": "-", "frame": 2, "gene": "ecfT", "product": "putative MG302-like protein", "db_xrefs": [ "COG:COG0619", "COG:H", "GO:0005886", "KEGG:K16785", "SO:0001217", "UniRef:UniRef50_P47544", "UniRef:UniRef90_P75357" ], "nt": "TTGGCCAGTCAAAATGAAAATTTTTTAGGTCATTTTATTTATTCAGGCGGTGAACAGTTTGGTGGGGTGAGTTGGTGGAGTGTCAATACCCGCTCTTTACTACGTTCATTGGTAATAGCACTACGAATTAGCATGCTGTTTGCCACGTCCTTTCTCTTAACTGCGTCCACTTCGATTTATGAGTTAGCGTTTGGGGTCGAACGGTTGTGTACCCCTTTGCGCTACCTCAAAATTAAAACACAACCGTTAAGCATCTTATTTGCACTGGTGTTTAAACTGTTACCCATTGTTAAGGGTGAATTAAAACGGATTAAACAAGCGCAAGCAATCCGTGGTTTTAAGTACGGTAAGCTAGCCTTTTTAAATCCAGTCAAACTCAAAACGCTCTTTATTCCGGTGTTATTGTCAACGGTCAAAAAAACCGAGGCAGTCGCGTTTGCGCTACAAGCCAAGGGTTACCAGTTGGACAATCCTAATAAAACGCACTATCTCCAAAAGTACAACCTTTGGGGTGGGATAGTCTTTCTGGGGCTTTTTGTCTTACTTAGTTGCTTATTAATGGTGAACAACTGA", "aa": "MASQNENFLGHFIYSGGEQFGGVSWWSVNTRSLLRSLVIALRISMLFATSFLLTASTSIYELAFGVERLCTPLRYLKIKTQPLSILFALVFKLLPIVKGELKRIKQAQAIRGFKYGKLAFLNPVKLKTLFIPVLLSTVKKTEAVAFALQAKGYQLDNPNKTHYLQKYNLWGGIVFLGLFVLLSCLLMVNN", "aa_hexdigest": "a75fbd3f2fd7e37bc8f9df0b6be3664d", "start_type": "TTG", "rbs_motif": null, "genes": [ "ecfT" ], "truncated": "both", "pseudogene": { "start": 518922, "stop": 519875, "observations": { "mutation": [ 519038, 519053, 519692, 519821 ], "stop": [ 519038, 519053, 519692, 519821 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75357", "query_cov": 1.0, "subject_cov": 0.5993690851735016, "identity": 0.995, "score": 365.0, "evalue": 7.46e-126, "gene_start": 1, "gene_end": 190, "reference_start": 85, "reference_end": 274, "reference_sequence": "MTSKNFLSQLPPVLKLWWWIISLVVAFLPLGLHGLIIINAIFFALVIVVERKLKTFAIIFGWLLFFFWFNIVVNGFIFLPNSSALASQNENFLGHFIYSGGEQFGGVSWWSVNTRSLLRSLVIALRISMLFATSFLLTASTSIYELAFGVERLCTPLRYLKIKTQPLSILFALVFKLLPIVKGELKRIKQAQAIRGFKYGKLAFLNPVKLKTLFIPVLLSTVKKTEAVAFALQAKGYQLDNPNKTHYLQKYNLWGGIVFLGLFVLLSCLLMVNNWHLVYWTNPHYSFTFTHQNFCFFKQISSPQLLAFWQLELLAIG" }, "paralog": false, "identity": 0.9873817034700315, "score": 616.0, "evalue": 5.89e-227, "description": "Internal stop codon at 519038, 519053, 519692, 519821. Nonsense mutation around 519038, 519053, 519692, 519821.", "psc": { "uniref90_id": "UniRef90_P75357", "gene": "EcfT", "product": "Uncharacterized protein MG302 homolog", "uniref50_id": "UniRef50_P47544", "cog_id": "COG0619", "cog_category": "H", "kegg_orthology_id": "K16785", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75357", "UniRef:UniRef50_P47544", "COG:COG0619", "COG:H", "KEGG:K16785", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47544", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47544" ], "product": "Uncharacterized protein MG302" } }, "id": "APKECPOBEN_941", "locus": "GCA002090215_00925" }, { "type": "cds", "sequence": "contig_1", "start": 519865, "stop": 520794, "strand": "-", "frame": 1, "gene": "ecfA2", "product": "Putative ABC transporter ATP-binding MG303-like protein", "db_xrefs": [ "COG:COG1122", "COG:PR", "GO:0005524", "GO:0016887", "GO:0042626", "GO:0043190", "SO:0001217", "UniRef:UniRef50_P75356", "UniRef:UniRef90_P75356" ], "nt": "GTGCACCCTTTTTATTATTTAACAGCTAAAAAACACACCCCGCTGTTTAACCAGCAGTTAGTTGATTTAGCGCAAAGTACGCTGTATTTTTACAATCTTAGTGTCTTTGTTGATAAAAGCAATGCTGGTCAAATTATTAAAAACGTTACCGGTAGTGTGGAACCTAACCAAATTACCGTTATTTTTGGTCCTAGTGGCAGTGGCAAAACGACCCTAATTAAACAGTTAGGTTTAGTTGAAAATCCGACCTGTGGTTTTTTGAACTGTGGTAACTTTTATTACTTTGCCAACCAAAAACATAACCGTGCCACAAAGCAGTTCCAAAACAGCATTGGCTATGTGTTACAAAAAGCCGAAGAACAGTTTTTCTGTGATTCCGTTTTAGAAGAGGTGTTAACGGGAGCGATTAACCTCGGTTTGTGTCAAAAGGGTGATGTCAACTTTGCCAAAAAGTACTTAGAGATGTGTGGTTTGGACCACATTCCCTTAATTAAAAACCCCTTGGAGTTGAGTGGCGGACAAAAGAAACGCTTGGCATTGGCGAGTGTATTAGCCATGCAAGTGCAATTTCTCATTTTGGACGAACCAACGGTCGGGTTAGACCAGGAGGGTAAAGCACTTAAAAGTGCTTTACTCAAACAATTAAAACAGGTCACGCGCATTATGATTGTCAGTCACGACGTTGATTTCATTTACGAAACCGCTGACAGTCTGATTCAATTAGAGGCTGGTCAAATTGTCGACCAAATGAGTGTTGCAGATTTCTTTAACAACATGCAGCTTTTACAACGGTACGAAATTACCCCACCACTTGTGGTGCAAACCATTCAGTTGTTACAAGCAAAGGGTGTGCAGTTAAACGATCCCTTAGCCATTAAAACCGTACACGATCTGATTGATCAACTCAAACCGCTCTTCCATGACCAGTAA", "aa": "MHPFYYLTAKKHTPLFNQQLVDLAQSTLYFYNLSVFVDKSNAGQIIKNVTGSVEPNQITVIFGPSGSGKTTLIKQLGLVENPTCGFLNCGNFYYFANQKHNRATKQFQNSIGYVLQKAEEQFFCDSVLEEVLTGAINLGLCQKGDVNFAKKYLEMCGLDHIPLIKNPLELSGGQKKRLALASVLAMQVQFLILDEPTVGLDQEGKALKSALLKQLKQVTRIMIVSHDVDFIYETADSLIQLEAGQIVDQMSVADFFNNMQLLQRYEITPPLVVQTIQLLQAKGVQLNDPLAIKTVHDLIDQLKPLFHDQ", "aa_hexdigest": "6fda083b9336e7474d6d4b2bc34b984a", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75356", "query_cov": 1.0, "subject_cov": 0.8089005235602095, "identity": 0.997, "score": 611.0, "evalue": 9.55e-220, "valid": true, "gene": "EcfA2", "product": "Putative ABC transporter ATP-binding protein MG303 homolog", "uniref50_id": "UniRef50_P75356", "cog_id": "COG1122", "cog_category": "PR", "go_ids": [ "GO:0043190", "GO:0005524", "GO:0016887", "GO:0042626" ] }, "pscc": { "uniref50_id": "UniRef50_P75356", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75356" ], "product": "Putative ABC transporter ATP-binding protein MG303 homolog" }, "genes": [ "ecfA2" ], "id": "APKECPOBEN_942", "locus": "GCA002090215_00926" }, { "type": "cds", "sequence": "contig_1", "start": 521010, "stop": 521720, "strand": "-", "frame": 2, "gene": "ecfA2", "product": "Putative ABC transporter ATP-binding MG304-like protein", "db_xrefs": [ "COG:COG1122", "COG:PR", "GO:0005524", "GO:0016887", "GO:0042626", "GO:0043190", "SO:0001217", "UniRef:UniRef50_P47546", "UniRef:UniRef90_P75355" ], "nt": "TTGCTCAACGTTACCAACCTCAGTTTTACCTATCCGCGCCACAGTCAACCGGCCTTAAAAAAGCTCAATTTTAGCCTCAAACCCAACACCCATGTAGCCATTATTGGCCACAATGGTTCGGGTAAATCGACACTGGTCAAACTGTTAGGCGGATTTTTAAAAGCACCGAAACAAACAATCTTTTTTAGGGGTCAACCCTTGGAAGAAGTTGGTTTTCGCCAAATTGGTATCTTACTGCAAGATCCCGACATGCAACTGTTGGGTGATACGCTGCACCAAGAGCTCATTTTTAGCTTGGAAAACCACGGGGTGGATCCTAAACAAATGGACCGAGTGATTGCTGATGTTTTAGCGGTTGTAGAACTACAAGGTAAACAGTTCACACCGCTCAGTAAGCTGTCGTTTGGCGAAAAACAACGGGCCGTGTTTGCTTGTTTACTGGCGGTGAAACCCGAACTTTATTTACTAGATGAAGCCTTTAGTATGTTGGATGGTAAGCTAGCTAGCAAACTAAAGCGGTTCATTTTTAAGGTAATTAAAGAACAGCAAAAAACAGTTATTAATGTTACCCATGACTTTAATGACCTCTTCTTAGCGGATGAAATTATCTTTTTAAGTAAGGGACAGCTGTTAAAACAGTTTTCACCCGCTGCCATATACAAACAGCTCCACTTGTTCCACCAGCACCACTTTACCCTCCCGTTTCCATGA", "aa": "MLNVTNLSFTYPRHSQPALKKLNFSLKPNTHVAIIGHNGSGKSTLVKLLGGFLKAPKQTIFFRGQPLEEVGFRQIGILLQDPDMQLLGDTLHQELIFSLENHGVDPKQMDRVIADVLAVVELQGKQFTPLSKLSFGEKQRAVFACLLAVKPELYLLDEAFSMLDGKLASKLKRFIFKVIKEQQKTVINVTHDFNDLFLADEIIFLSKGQLLKQFSPAAIYKQLHLFHQHHFTLPFP", "aa_hexdigest": "8ce67e03e31af7f4b5a49b1a278319f6", "start_type": "TTG", "rbs_motif": "AAAA", "psc": { "uniref90_id": "UniRef90_P75355", "query_cov": 1.0, "subject_cov": 0.8676470588235294, "identity": 0.992, "score": 464.0, "evalue": 1.01e-164, "valid": true, "gene": "EcfA2", "product": "Putative ABC transporter ATP-binding protein MG304 homolog", "uniref50_id": "UniRef50_P47546", "cog_id": "COG1122", "cog_category": "PR", "go_ids": [ "GO:0043190", "GO:0005524", "GO:0016887", "GO:0042626" ] }, "pscc": { "uniref50_id": "UniRef50_P47546", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47546" ], "product": "Putative ABC transporter ATP-binding protein MG304" }, "genes": [ "ecfA2" ], "id": "APKECPOBEN_943", "locus": "GCA002090215_00927" }, { "type": "cds", "sequence": "contig_1", "start": 521938, "stop": 523665, "strand": "-", "frame": 1, "gene": "dnaK", "product": "molecular chaperone DnaK", "db_xrefs": [ "COG:COG0443", "COG:O", "GO:0005524", "GO:0006457", "GO:0051082", "GO:0140662", "SO:0001217", "UniRef:UniRef50_P9WMJ9", "UniRef:UniRef90_P47547" ], "nt": "ATGAGTACAGATAACGGCTTAATTATCGGCATTGACCTTGGTACCACTAACTCCTGTGTGTCGGTCATGGAGAATGGACGCCCAGTAGTGTTGGAAAACCCTGAAGGTAAACGCACCACCCCTTCGATTGTTTCTTACAAGAACAACGAAATTATTGTGGGTGATGCTGCGAAACGGCAAATGGTAACTAACCCTAATACTATTGTTTCCATTAAGCGTTTAATGGGTACCTCCAATAAGGTAACCGTTAAGAATCCTGATGGTTCTACCAAAGAGTTAACTCCTGAAGAGGTATCAGCGCAAATCTTGAGCTACCTCAAGGACTATGCGGAAAAGAAGATTGGTAAAACGATTTCCCGTGCTGTTATTACCGTACCTGCTTACTTTAACGATGCAGAACGGAACGCTACTAAAACCGCTGGTAAGATTGCTGGTTTAAACGTTGAGCGGATTATTAACGAACCTACCGCCGCTGCATTGGCTTATGGGATCGACAAGTCTAACCGAGAAATGAAAGTCTTGGTGTACGACCTTGGTGGTGGTACCTTTGACGTTTCCTTACTTGACATTGCTGAAGGTACCTTCGAAGTATTAGCCACTGCTGGGGACAACCGTTTGGGTGGTGATGACTGGGACAACAAGATTATTGAGTTCATCTTAGCGCACATTGCCCAAGAACACAATGGGCTTAACTTGTCCAATGACAAGATGGCTATGCAACGCTTAAAGGAAGCGGCTGAACGTGCTAAGATTGAACTTTCCGCCCAACTAGAAGCAATTATCTCTTTACCGTTCTTAACGGTTACCGAAAAGGGTCCGGTAAACGTTGAACTTAAGCTAACCCGTGCTAAGTTTGAAGAAATTACCAAACAATTACTAGAACGTACTCGCAACCCAATTTCGGATGTTTTACGTGAAGCCAAGATTAAACCAGAAGAAATTAATGAAATCTTGTTGGTGGGTGGATCGACCCGGATGCCAGCAGTGCAAAAACTAGTGGAATCAATGGTACCAGGACACAGTCCAAACCGCTCAATTAACCCGGATGAGGTGGTAGCCATTGGTGCTGCCATCCAAGGGGGTGTGTTACGCGGTGATGTAAAGGACGTGTTACTGTTGGACGTTACTCCTTTAACGCTCTCGATTGAAACCCTTGGTGGTGTAGCAACTCCGTTAATTAAGCGTAACACCACCATTCCTGTAAGTAAGAGTCAAATCTTCTCTACAGCGCAAGACAACCAAGAATCAGTGGATGTGGTGGTTTGTCAAGGGGAACGCCCAATGGCACGTGACAACAAGTCTTTGGGTCGCTTTAACTTAGGGGGCATCCAACCAGCACCCAAGGGTAAACCCCAAATTGAAATTACCTTTAGCTTGGACGCCAACGGGATCTTAAACGTGAAGGCTAAAGATTTAACCACTCAAAAGGAAAACAGTATTACTATTAGTGACAACGGCAACTTGTCCGAAGAGGAAATCCAAAAGATGATTCGTGATGCGGAAGCCAACAAGGAGCGTGACAATGTGATTCGTGAACGCATTGAGCTCCGTAACGAAGGTGAAAGCATCGTGAGCACGATTAAGGAGATTCTCCAAAGTCCCGAAGCGAAGGACTTCCCTAAAGAAGAGAAGGAAAAACTCGACAAGATTACCGGTGGTATTGATGCAGCAATTAAGGCCAATGACTACACCAAGTTAAAAGCCGAAATCGAAAACTTCAAGAAGTGA", "aa": "MSTDNGLIIGIDLGTTNSCVSVMENGRPVVLENPEGKRTTPSIVSYKNNEIIVGDAAKRQMVTNPNTIVSIKRLMGTSNKVTVKNPDGSTKELTPEEVSAQILSYLKDYAEKKIGKTISRAVITVPAYFNDAERNATKTAGKIAGLNVERIINEPTAAALAYGIDKSNREMKVLVYDLGGGTFDVSLLDIAEGTFEVLATAGDNRLGGDDWDNKIIEFILAHIAQEHNGLNLSNDKMAMQRLKEAAERAKIELSAQLEAIISLPFLTVTEKGPVNVELKLTRAKFEEITKQLLERTRNPISDVLREAKIKPEEINEILLVGGSTRMPAVQKLVESMVPGHSPNRSINPDEVVAIGAAIQGGVLRGDVKDVLLLDVTPLTLSIETLGGVATPLIKRNTTIPVSKSQIFSTAQDNQESVDVVVCQGERPMARDNKSLGRFNLGGIQPAPKGKPQIEITFSLDANGILNVKAKDLTTQKENSITISDNGNLSEEEIQKMIRDAEANKERDNVIRERIELRNEGESIVSTIKEILQSPEAKDFPKEEKEKLDKITGGIDAAIKANDYTKLKAEIENFKK", "aa_hexdigest": "c4c9d98b7612066c9a72eb32f77d0427", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47547", "query_cov": 1.0, "subject_cov": 0.9663865546218487, "identity": 0.934, "score": 1022.0, "evalue": 0.0, "valid": true, "gene": "dnaK", "product": "molecular chaperone DnaK", "uniref50_id": "UniRef50_P9WMJ9", "cog_id": "COG0443", "cog_category": "O", "go_ids": [ "GO:0005524", "GO:0006457", "GO:0051082", "GO:0140662" ] }, "pscc": { "uniref50_id": "UniRef50_P9WMJ9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P9WMJ9" ], "product": "Chaperone protein DnaK" }, "genes": [ "dnaK" ], "id": "APKECPOBEN_944", "locus": "GCA002090215_00928" }, { "type": "cds", "sequence": "contig_1", "start": 523880, "stop": 524197, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTATGGTTTAGAAATTAAACGTTTAGGCTTGACGATCTTCAAATTACTAGCCAATATCTTAATAGCAGTGGTGGGTTACTTTAGTGTGTTTGCTGTTTTTGCCATTGTCAAAAAGGAAAACGCAACTAACAACGTTATTGACCAGTACTCTACGGCTGACATTTTCAACATCACTAACATTGCCATTACCGCTTCTAACTTTATTCCCGTACCTAGTGGGGAAGGTGCTACCCAGTTTGTAATGACAAGCTTTTTAAATGCCTTTAAATCGGCTGTTGGTATTGAATCGCAAGTAAAGCAAGGGGTCTTTTTGTGA", "aa": "MYGLEIKRLGLTIFKLLANILIAVVGYFSVFAVFAIVKKENATNNVIDQYSTADIFNITNIAITASNFIPVPSGEGATQFVMTSFLNAFKSAVGIESQVKQGVFL", "aa_hexdigest": "706b7193aaa74d2f01bbae0e318addc0", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11385.160599999992, "isoelectric_point": 8.00776309967041 }, "id": "APKECPOBEN_945", "locus": "GCA002090215_00929" }, { "type": "cds", "sequence": "contig_1", "start": 524267, "stop": 524368, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATCTTTGATGTGGTGGTAGCCATCTTATTTATCTTTATTGCCTACAGTAAAAGGATGCATGTCTTAATTTACTCCGGGGTAAACCAGTTTCGTAAGTGA", "aa": "MIFDVVVAILFIFIAYSKRMHVLIYSGVNQFRK", "aa_hexdigest": "38a2d1f03e38d08d6a172cce833d66d7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3919.7433, "isoelectric_point": 9.992035484313966 }, "id": "APKECPOBEN_946", "locus": "GCA002090215_00930" }, { "type": "cds", "sequence": "contig_1", "start": 524456, "stop": 524611, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCAGTTCTTTCAAATTGTCACCCCACTTTCCTTGGGTCAAGATCCCTTTCGCTTGTACTGGTTCATTAAAAAGGGCATGAAGAAGCAGACAGCGGTCTTATTAGTTACTTCCACGGGCGCCTTTTGGAACCTTGCCCAAGCGTTAATTACTTGA", "aa": "MQFFQIVTPLSLGQDPFRLYWFIKKGMKKQTAVLLVTSTGAFWNLAQALIT", "aa_hexdigest": "a1c0539050506c6ffe39ed05945a04fb", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5848.919200000001, "isoelectric_point": 10.171064186096192 }, "id": "APKECPOBEN_947", "locus": "GCA002090215_00931" }, { "type": "cds", "sequence": "contig_1", "start": 524741, "stop": 524908, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAACCCCTAAAAGCGCTAGTTTTTTTACTAGAAAAAATATTCTTGCCTTTAGCTTTTTTATTGCCTTTTTGGTGGTTGTTAGTGTATTGGTGACCGTCTTCTTTTTAGACATTAAAACTGGTGATGTTAAAACGATTATTAACACCATTAACAGAACTAACTGA", "aa": "MSTPKSASFFTRKNILAFSFFIAFLVVVSVLVTVFFLDIKTGDVKTIINTINRTN", "aa_hexdigest": "e0141c928abccabe149aedb1b9e4099d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6174.254499999999, "isoelectric_point": 10.446408653259276 }, "id": "APKECPOBEN_948", "locus": "GCA002090215_00932" }, { "type": "cds", "sequence": "contig_1", "start": 524918, "stop": 526372, "strand": "-", "frame": 3, "gene": null, "product": "putative protein MPN_437", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75341" ], "nt": "TTGGAAAACATCCAAACTAAAGTAAATGATTTTGTTAAAAACAAACTCAAACCTGCTTTAGCACCCCGACCAGCTTACAGTAACGTCATTTTGTTAAACGTTAACAATGACAAAGTACTTTCTAGTGGTGCTAACTGGAGCTTAGCTAGCTTATTACAGAGCGACAAAGTCAACCCGCTTTCGTTTATGTTGCTCAAACAAGCGTTCGATAATAACGATTTATTTAAGAAAGCACAAAAGTTGTTTAAAGACATTCAGGAAAAGTCCAGTAATAATGGTGGAATGCAAAGTAGTTCCACAACCAACAGTGATGCCGATGCATTAAGTAAAGTTATCGGTAACTATTACTACACTACTTGGGCTAAACTCACGGACAAATCGATTTACGGTAACCCGAAGGACAACAAGTTTGATGAGCTTTTCAAACTGGCTTTTGAAGCTAGCATCGACGAAAAGTCCTTTAACGTTGATTACAAAGCGGTCATTGACCATTACCGTTTTATCTATACCTTACAGTGGCTAGTGGACCAAAAATTAAAGAACTTTAAGTCACTGTTAAAAACAAACCTTAAGTTTGGTGAAGTTGCTTTTATAGCTTACAAGAACACTGAAACCACTAACTTCTCTAATCCCCAAGGCGTATTTGGTTCCTACTTCAACTATGAGAACTCCGCTAACGAAGTTAAAGAATCCACTCAAACACTAGATCCCAATAACTTCTTTTACAAAACAACAACCAAGCCAACGGTACAAGCCATTCAACAAGTTGCTAGCTTAGCATTAGTACAAAAACAACAAATGCAACAAAATTCAACTGATCACTATGGTTTTACTGGTTTGAGTACCAGCACTAGTTCGATGTTCGATGCTAGTTCCCGTGATGCCATTTTGCAGCAAATTACGAAAACCTCCTTGCAACAGTACGGTTCCAAGGATCAACTCAAAAAGATCATTCAAGGGACTAATAACCAATTGTTACTAGACCGGATTGCAGTCCAGTTAAGTGGATTAAATCCTTCCACAACTAATGGTGGCAGTGGCAAAACAATTGCGACCTACTTCCAAGTGGATGCCGTTGGCAACCCAACTTTGGACTTCCAAGCTAAGCGTAAACTGTTATTAGACCTCTTGGACCAGTACCAAAACTACTTTGGTAATGGTGCCCAAAAGAGCCAAAGGGATTCTACTCCAAGTGGAACTGGCAACTACCTCACTTACCAAAATGGTAGTGACAAGTACACCTACACCCAGTTCACTTACCAAGACATTGACAGCTTGAGTCTAACAACTACAAGCGGTACCAACAATAAAATTGCCAGTGATGTTGTGGCAGCGCTGCTTTTATTCCAAGCAGCAGACAAGGGCACACAACAGTTGGCACTTAGTGCCATTAATAAGCCGCAGTTAAATATTGGTGATAAACGGATAGAAAGTGGGTTAAAATTGCTCAAATAG", "aa": "MENIQTKVNDFVKNKLKPALAPRPAYSNVILLNVNNDKVLSSGANWSLASLLQSDKVNPLSFMLLKQAFDNNDLFKKAQKLFKDIQEKSSNNGGMQSSSTTNSDADALSKVIGNYYYTTWAKLTDKSIYGNPKDNKFDELFKLAFEASIDEKSFNVDYKAVIDHYRFIYTLQWLVDQKLKNFKSLLKTNLKFGEVAFIAYKNTETTNFSNPQGVFGSYFNYENSANEVKESTQTLDPNNFFYKTTTKPTVQAIQQVASLALVQKQQMQQNSTDHYGFTGLSTSTSSMFDASSRDAILQQITKTSLQQYGSKDQLKKIIQGTNNQLLLDRIAVQLSGLNPSTTNGGSGKTIATYFQVDAVGNPTLDFQAKRKLLLDLLDQYQNYFGNGAQKSQRDSTPSGTGNYLTYQNGSDKYTYTQFTYQDIDSLSLTTTSGTNNKIASDVVAALLLFQAADKGTQQLALSAINKPQLNIGDKRIESGLKLLK", "aa_hexdigest": "f4c3c2c83d51ef7096838343ed9540bf", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75341", "query_cov": 0.9772727272727273, "subject_cov": 0.8269230769230769, "identity": 0.721, "score": 635.0, "evalue": 1.6e-223, "valid": false, "product": "Uncharacterized protein MPN_437", "uniref50_id": "UniRef50_P75341" }, "pscc": { "uniref50_id": "UniRef50_P75341", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75341" ], "product": "Uncharacterized protein MPN_437" }, "genes": [], "id": "APKECPOBEN_949", "locus": "GCA002090215_00933" }, { "type": "cds", "sequence": "contig_1", "start": 526466, "stop": 527554, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCGCAAATTAAGGAGCTCAAAGAAGACCTTAACAACACGATTGTTGTCGATAAAAGTAAGGACGTAGAAAAAGCTTCCAAAACTAACGCACTGTTTTACAATGATCAAGAAGGTAAGCAACAACAAAGTGACTCAGATCCAATTGCTGGCGCTTTAGATGACATTTTTGCTCAAAACACAAGCGAAGGCACTAACTTAAGTAAGTTAGCGGAGCAGGTAAAGAAAGCAGCTGCAACAAAAATGGAAGCGAAAACAGCTGTTTTAAGAACTAACAATTCTAAGGGCCAACAAAACAATTACGTTGTTTTAGATGCAGCTATTCCTACATTTAATTCAACAACATCAAAATCGAAAAATAATAGCGCTTCTAATGAAGTTTTAGTTGCCTTAAAATCTGGATCTATAAATTTAAGGCAGGTTCAGCAAACTGATCAAAACAGTTACAGTCCCATTAAATTCCGGATTGTGCGTAACAGCACCGGAGTAACTGTTTTCGGCCTTGATGGTGGGAGCTATTATTTAAAACAAGATTCAACAAATAAAAAATCAGTTTCTAAGCAAAGTTTAACCTTATTAACTAAATCTAGTTCAGGTAACAGTAATAAAGTATTAAGAGACCTTGACAAGCAAAAACAATTCTTAAAGTTTCGTGCCTTTCAAGCTAAAACTAACACTTTCTACAGTACTAACTTTGCCTTTAGTTTTCCCTTAAACGAAACGCTAAAAAGTTGGTTTGATAAACACCGGGAACTAATTTTGGCCAACGCCTTAGTTAACGCTAGTCTTGACCAAAAGGATAAAGCTAGCAAAGCTTTAACTGAAGCTTTTAATCCTTATAAAGAGTTAATTAAAGAATTTGCGCCGGTGGCTTTAGCAACCACAATGATCAGCTTTTATTTTGATCAAATGAAAGCGCTCAATAACAAGTTGCTAGAGCGCGCCCGTAATCTTAACCAAAACGTCAACCAAGCCAATCCAACCCCTTGGTTGAACGGTTTGTCAGCCAAGTTACCTTACGTTAATACTAACGGTAATTACGAAAAGTTAAACAACTACTTTACCTTCTTAATCACAAAAACATTGTGA", "aa": "MPQIKELKEDLNNTIVVDKSKDVEKASKTNALFYNDQEGKQQQSDSDPIAGALDDIFAQNTSEGTNLSKLAEQVKKAAATKMEAKTAVLRTNNSKGQQNNYVVLDAAIPTFNSTTSKSKNNSASNEVLVALKSGSINLRQVQQTDQNSYSPIKFRIVRNSTGVTVFGLDGGSYYLKQDSTNKKSVSKQSLTLLTKSSSGNSNKVLRDLDKQKQFLKFRAFQAKTNTFYSTNFAFSFPLNETLKSWFDKHRELILANALVNASLDQKDKASKALTEAFNPYKELIKEFAPVALATTMISFYFDQMKALNNKLLERARNLNQNVNQANPTPWLNGLSAKLPYVNTNGNYEKLNNYFTFLITKTL", "aa_hexdigest": "aa66e56c52c222435d22bef84a58192e", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 40430.09539999999, "isoelectric_point": 9.588463401794431 }, "id": "APKECPOBEN_950", "locus": "GCA002090215_00934" }, { "type": "cds", "sequence": "contig_1", "start": 527816, "stop": 528001, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGAGTACACTGATCCCACTTTAATTAGTCAAGTTAACTATAAGTATTCTGCTCCCAGTCAAGGGTTAGGTCAGATCTATAACAGAGAGAAGTTAAAGGATAAACTAACACCTTCTTATGCCTTTCCTTTCTTTGCCGAAGAAAAAGACATTGCACCCAACCAAAACGTTGGTAATAAGCGCTGA", "aa": "MEYTDPTLISQVNYKYSAPSQGLGQIYNREKLKDKLTPSYAFPFFAEEKDIAPNQNVGNKR", "aa_hexdigest": "cf66cb80295171112421c4c0f42a0c3f", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7015.779500000002, "isoelectric_point": 7.961990547180176 }, "id": "APKECPOBEN_951", "locus": "GCA002090215_00935" }, { "type": "cds", "sequence": "contig_1", "start": 528089, "stop": 528454, "strand": "-", "frame": 3, "gene": null, "product": "putative lipoprotein MPN_439", "db_xrefs": [ "GO:0098552", "SO:0001217", "UniRef:UniRef50_P75339", "UniRef:UniRef90_P75339" ], "nt": "GTGGCCAACAAACAATTTATCGCTGCACCACTTTTAAAAGCACTAGAAGCTTGGTACGAAAACAACGAAGACAAAAAGATTACCCAGTTTTTAAAAGACACTAAGTCCAATGTTGACAGTCAGTACACAACGGCAGTCGATAAAGTGGTATCAGCATCACGCAATAAATCACTTTTTGTGCAACAAGATTTGTTGGATAACGCCGGTGGTAGTGAAGCAACCTGGAAAGCGCAAAAGCTGCTTGAACAGCTCATTAGTGACTTTGCTAGTCGGGTTTTCCAAAAGAACTACCTCAATTACAAAAAAGATGGACAAGTTTCTACTGGTCCATTTACTTATGATGAACTACACAAGGAAGAAAGCTGA", "aa": "MANKQFIAAPLLKALEAWYENNEDKKITQFLKDTKSNVDSQYTTAVDKVVSASRNKSLFVQQDLLDNAGGSEATWKAQKLLEQLISDFASRVFQKNYLNYKKDGQVSTGPFTYDELHKEES", "aa_hexdigest": "f6b67903329ec1df8755fa34cab58ee5", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 527826, "stop": 528652, "observations": { "deletion": [ 527866 ], "mutation": [ 528091, 528622 ], "stop": [ 528091, 528622 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75339", "query_cov": 0.9586776859504132, "subject_cov": 0.4218181818181818, "identity": 0.81, "score": 192.0, "evalue": 1.8e-59, "gene_start": 3, "gene_end": 118, "reference_start": 69, "reference_end": 184, "reference_sequence": "MKSFLRKPKFWLLLLGGLSTSSIILSACATPSNSALQAVFKPTSNQFFNGEHGTIQSALNTALRDPETNKKFVAAPLLKALEAWYENNQDKNITQFLKDTKTNVDNQYKTVVDKVVSAPRNKSLFVQQDLLDSSGGSEATWKARKLFEQLISDFASRVFQKNYLSYKENGKVSAGPFLYDTISKNSNWQNIVFDAGNFPETNDDFFAQIQSEVFDQWAEYTDPTIISSVTLKYSAPNQGLGQIYNKDKLKDKLTPSYAFPFFCWGKRYSGKSKRW" }, "paralog": false, "identity": 0.7381818181818182, "score": 414.0, "evalue": 7.4e-149, "description": "Internal stop codon at 528091, 528622. Frameshift due to deletion around 527866. Nonsense mutation around 528091, 528622.", "psc": { "uniref90_id": "UniRef90_P75339", "product": "Uncharacterized lipoprotein MPN_439", "uniref50_id": "UniRef50_P75339", "go_ids": [ "GO:0098552" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75339", "UniRef:UniRef50_P75339", "GO:0098552" ] }, "pscc": { "uniref50_id": "UniRef50_P75339", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75339" ], "product": "Uncharacterized lipoprotein MPN_439" } }, "id": "APKECPOBEN_952", "locus": "GCA002090215_00936" }, { "type": "cds", "sequence": "contig_1", "start": 528962, "stop": 529588, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTATGCAACAAAAAATGCAAAGTACCATGCAAGATAGTAATCATTATGGTTTTACCGGGCTAAACACCAGTACTAGTTCAATGTTAGGTGCCGCTACGCAGCAAGCCATTTTGGATCAAATAACCAAGAATTCCTTGCAACAATATGGTTCGCAGCAAGAACTGAAAACTTTGATTGAAAAGACTAATAACCAACTTTTATTAGACCGAATCGCTAGTCAATTAAGTGGATTAAATCCTTCAACCACTGGAAATAGTAATAATGGAAAGGGTAAAAATATTGCTACTTATTTCCAATTAGATGCCATTGGCAACCCAACCCTTAGCTTTCAACAAAAACGAAAACTTTTATTAGATGTTTTAGATCAGTACAAAGATTTCTTTGGTACAAATACTCAGGCTGCACAAAGAGATTCTGGTAAAGGTGGACATGGAAGCTATTCAACTTACCAAGATGGCAGTGACAAGATCACTTACCTTCAGTTCTCCTATAAAGATATAGATAACTTAAGTTTGAGTGATAAAGGAAATAGTAAGCTTGCTAGCGATGTTGTGGCTGCCCTTTTACTTTTCCAAGCAGCGGATAAAGGTACCCAACAATTAGCCTTGAGTGCTATTAATTAA", "aa": "MVMQQKMQSTMQDSNHYGFTGLNTSTSSMLGAATQQAILDQITKNSLQQYGSQQELKTLIEKTNNQLLLDRIASQLSGLNPSTTGNSNNGKGKNIATYFQLDAIGNPTLSFQQKRKLLLDVLDQYKDFFGTNTQAAQRDSGKGGHGSYSTYQDGSDKITYLQFSYKDIDNLSLSDKGNSKLASDVVAALLLFQAADKGTQQLALSAIN", "aa_hexdigest": "e7819ac808059bfc7a5c34ffcba198d0", "start_type": "ATG", "rbs_motif": "AAAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 22667.97439999998, "isoelectric_point": 7.837953376770019 }, "id": "APKECPOBEN_953", "locus": "GCA002090215_00937" }, { "type": "cds", "sequence": "contig_1", "start": 529850, "stop": 530002, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGACTGATAAATCCATTTATGGAAATCCTAAGGACAACAAATTTGATGACTTATTTAAATTAGCTTTTGAAGATAGTATTAACGAAAAGTCCTTTAATGTTGATTACAAAGCGGTCATTGAACACTACCGCTTTATCTATACCTTGGAATGA", "aa": "MTDKSIYGNPKDNKFDDLFKLAFEDSINEKSFNVDYKAVIEHYRFIYTLE", "aa_hexdigest": "6489b238ee66db2e3891a3bff0400f39", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6013.649899999999, "isoelectric_point": 4.9303506851196275 }, "id": "APKECPOBEN_954", "locus": "GCA002090215_00938" }, { "type": "cds", "sequence": "contig_1", "start": 530009, "stop": 530191, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGCTCAAACAAACGTTTGACCAAAACGACTTGTTTAAGAAAGCTAAGAAACTTTTTGAAGATATTCAAAGTAAAACAAATGGTGGAAGTTCAGGTGGGATGCAAGGTAGCAATACCTCGAGTAGTGAAGGAGCTGATGCCTTGAGTAAAGTAATTGGCAACTATTACTACAACAGTTGA", "aa": "MLLKQTFDQNDLFKKAKKLFEDIQSKTNGGSSGGMQGSNTSSSEGADALSKVIGNYYYNS", "aa_hexdigest": "162176d8455fd8b24da4fbb08368e614", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6501.096200000004, "isoelectric_point": 7.9830717086792005 }, "id": "APKECPOBEN_955", "locus": "GCA002090215_00939" }, { "type": "cds", "sequence": "contig_1", "start": 530258, "stop": 530794, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTAAAGGCAAATTTTGATCAAAAGGATAAAGCAAACAAAGCTTTAACTGAAGCCTTAAAGCCTTACAAGGATTTACTGGCAGAATATGCACCAATTGGTTTGGCCACAACCATGATTAACTTCTTGTTTAACCAAATGAACGCGTTGAACAATAAGTTTTTGGAGCGCGCTAAAGCATTAAACCAAAATGTGAATCAGGCCAATCCTACACCTTGGTTAAATGGTTTATCAGCTAAATTGCCTTATGTTAGAACAAATGGTAATTACGAAAAACTCAACAATTACTTCACTTTCTTAATTGTTAAATACATGTGGAAGAAGGTCGGTAATGAAGACGCTTCATTATCTAAAGATAGTAGCATTAACAAGCTCAAAACTAAGACCGAAGACGTTAACAAAATTAGAGATAAAATCTTAGAAGACATTCAAAAGAAGGTTCAAGAATTTGTTAAAAACAAGCTTAAACCAACCTTAGCACCACGACAAACTTACAGTAATGTAATTTTGTTAAACGTTAACAACGATAAGGTTTGA", "aa": "MVKANFDQKDKANKALTEALKPYKDLLAEYAPIGLATTMINFLFNQMNALNNKFLERAKALNQNVNQANPTPWLNGLSAKLPYVRTNGNYEKLNNYFTFLIVKYMWKKVGNEDASLSKDSSINKLKTKTEDVNKIRDKILEDIQKKVQEFVKNKLKPTLAPRQTYSNVILLNVNNDKV", "aa_hexdigest": "2e2ca51d87ffdf207bfa500b608cab5a", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 20429.38020000001, "isoelectric_point": 9.718044853210447 }, "id": "APKECPOBEN_956", "locus": "GCA002090215_00940" }, { "type": "cds", "sequence": "contig_1", "start": 530898, "stop": 531287, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCACAATCTGATGAAAACTATTTGTTATTAGATGCTGCGATCCCGGCGTTTGAAACTTCTTCACAATCACCACAAAGCAGTGGAACTATTTCTGGTAGTGGCGCTTTAGTTAATTTGACTTCTACTAGCGTCAATTTAACTCAACAAAAAAGTAGTACAACGCCTTTAAACCAGCGCATTGTTCGAAATAGTACTGGTGTAAAATTGCTGGCTCTTGAAGGCTTGCTTTACTACGTTGTTGGATCTTCCAATAAACAACAAAGTAGCTCTTCTACGATAGCCACTTCTCTCGCTCAAAACCAAAGTAATAATAATCAAGAACAAGAGATTAATAGCAGTCTTCAAAAAAATTTATTGAAGTTTCGTGCCTTTCAAGCTAAAAATTAG", "aa": "MAQSDENYLLLDAAIPAFETSSQSPQSSGTISGSGALVNLTSTSVNLTQQKSSTTPLNQRIVRNSTGVKLLALEGLLYYVVGSSNKQQSSSSTIATSLAQNQSNNNQEQEINSSLQKNLLKFRAFQAKN", "aa_hexdigest": "c1a660ba6aa31b11f4f42e7391c7b7ee", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13800.075499999986, "isoelectric_point": 9.09901943206787 }, "id": "APKECPOBEN_957", "locus": "GCA002090215_00941" }, { "type": "cds", "sequence": "contig_1", "start": 531324, "stop": 531569, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATGACACCATTGTAGCTAAATCTGATAACTTAGAGAAAACTTCTAAAACTAACAGTACTTTTTACAAAGATAATGGTGATAGTAGCGAAAAACAAGGAAGTGCTGAAAGCAAAAATTCACCTAAAACTCAATTAAAGGAAATTTTTAATGGTCAAAATGGCGATAGTGGTAATTTAGGTAAGCTAGCTGAACAGTTACAAAAAACTGACAGTACTAAAATGGAAGCTAGAAGTCAGACATGA", "aa": "MNDTIVAKSDNLEKTSKTNSTFYKDNGDSSEKQGSAESKNSPKTQLKEIFNGQNGDSGNLGKLAEQLQKTDSTKMEARSQT", "aa_hexdigest": "df552884f2d00cd94024786ab02edb11", "start_type": "TTG", "rbs_motif": "AAAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8832.463799999998, "isoelectric_point": 6.217185401916503 }, "id": "APKECPOBEN_958", "locus": "GCA002090215_00942" }, { "type": "cds", "sequence": "contig_1", "start": 532282, "stop": 532665, "strand": "-", "frame": 1, "gene": null, "product": "putative lipoprotein MPN_442", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75336" ], "nt": "TTGTTGGGTGGCCTTTCCACTAGTAGTATTATTCTCAGTGCCTGTGCAACACCTTCAAACTCAGCACTGCAAGCAGTTTTTAAACCAACTTCCAACCAATTTTTTAACGGTGAGCATGGTACCATTCAAAGCGCTTTAAACACCGCTTTAAGAGATCCGGAAACTAACAAAAAATTTGTAGCTGCTCCACTTTTAAAAGCATTAGAAGCTTGGTACGAGAATAATCAAGATAAAAACATTACTCAATTCTTAAAAGACACCAAGACTAATGTTGATAACCAATACAAAACCGTTGTAGATAAAGTAGTTTCAGCACCACGCAATAAATCCTTATTTGTACAACAAGATTTATTGGACAGTAGCGGTGGTAGTGAGGCCACTTGA", "aa": "MLGGLSTSSIILSACATPSNSALQAVFKPTSNQFFNGEHGTIQSALNTALRDPETNKKFVAAPLLKALEAWYENNQDKNITQFLKDTKTNVDNQYKTVVDKVVSAPRNKSLFVQQDLLDSSGGSEAT", "aa_hexdigest": "3c229614f401cf9a42d083d70b5c7567", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75336", "query_cov": 0.9606299212598425, "subject_cov": 0.8079470198675497, "identity": 0.762, "score": 182.0, "evalue": 4.37e-57, "valid": false, "product": "Uncharacterized lipoprotein MPN_442", "uniref50_id": "UniRef50_P75336", "go_ids": [ "GO:0098552" ] }, "pscc": { "uniref50_id": "UniRef50_P75336", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75336" ], "product": "Uncharacterized lipoprotein MPN_442" }, "genes": [], "id": "APKECPOBEN_959", "locus": "GCA002090215_00943" }, { "type": "cds", "sequence": "contig_1", "start": 532860, "stop": 533891, "strand": "-", "frame": 2, "gene": null, "product": "putative protein MPN_440", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75338", "UniRef:UniRef90_P75338" ], "nt": "TTGGCTTTTGACAGTAGTATTAACGAAAAGTCCTTTAATGTAGATTATAAGGCAGTGATTGAACACTACCGCTTTATCTATACCTTAGAGTGGTTGGTAGACAAAAACCTAAAGAACTTCAAGGATTTATTAAAGGCAAACCTCAAGTTTGGTGAAATTGCTTTTATTGCTTACAAGAATACTGAAACGCAGAACTTCTCTAACCCGCAAGGTATATTCGGTTCTTACTTTAATTACGAAAACGAAACGAATGCGGCTAAGAGTGCTACGCAAATTATAGACCCCAACAGTTTCTTCTATAAAACCACCACTAAACCAGAAGCGAAAACCACTCAAAGTGCTAATACAGCTGTGATGGTTCAAAATACGCAGATGAACAATCAGCAAACTAACAGTTATGGCTTTACTGGTTTGAGTACCAGTAGTGGTTCGATGTTAGGTGCTGCTACCCAGCAAGCCATTTTGGATCAAATAACCAAAACTTCCTTGCAACAGTATGGTTCTCAAGCTGACCTGAAAAAGATCATTGGCGAAACTAAAAATCAATTATTATTAGACCGAATTGCCAACCAACTAATAGCCTTAAAACCGAATACAAGTGGCAACAGTGGTACCCAAAAAACAATTGCTGCATACTTCCAAACAGATGCGGTTGGCAATCCTACTTTGGACTTTAAAGCGAAGCAAAAACTCTTATTGGATGTTTTAGATCAATACAAAGATTTCTTTGGTAATAATGCACAAGCAGTTCAAAGAGATTCTGGTAAGAGTGGAACTGGCAACTATTTAACCTATACCGACGGTAGTGATAAGATCACTTATTTGCAGTTTTCCTATAAAGATATTGACGGTTTAAGTTTGAGTAGTTCAAACGGAACTAGCAGCAAATTTGCCAGTGATGTTGTAGCAGCGCTTTTATTATTCCAGGCAGCCTATAAAGGTACTCAACAACTGGCTTTAAGTTCCATCAATAAACCACAATTACCAATTGGCGATAAACGCATAAAAACAGGGATCGATTTACTGAAATAG", "aa": "MAFDSSINEKSFNVDYKAVIEHYRFIYTLEWLVDKNLKNFKDLLKANLKFGEIAFIAYKNTETQNFSNPQGIFGSYFNYENETNAAKSATQIIDPNSFFYKTTTKPEAKTTQSANTAVMVQNTQMNNQQTNSYGFTGLSTSSGSMLGAATQQAILDQITKTSLQQYGSQADLKKIIGETKNQLLLDRIANQLIALKPNTSGNSGTQKTIAAYFQTDAVGNPTLDFKAKQKLLLDVLDQYKDFFGNNAQAVQRDSGKSGTGNYLTYTDGSDKITYLQFSYKDIDGLSLSSSNGTSSKFASDVVAALLLFQAAYKGTQQLALSSINKPQLPIGDKRIKTGIDLLK", "aa_hexdigest": "9d490c95a5151e7b6106bbc1f002fe9e", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 532860, "stop": 534191, "observations": { "mutation": [ 533960, 534182 ], "stop": [ 533960, 534182 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75338", "query_cov": 1.0, "subject_cov": 0.4724517906336088, "identity": 0.997, "score": 653.0, "evalue": 1.86e-230, "gene_start": 1, "gene_end": 343, "reference_start": 384, "reference_end": 726, "reference_sequence": "MQQQGETKDQYNTFGLRLVRNSVGVSVLGLDGFVKFIKGGSGGSNGGSSSAKKIDKEEQKKFLKFRAFQAKIGTFYNTNFAFSFPLNETLKGWFDKHRGLILANALVKVTLDTKEKASKALVDAFSSYKNWLSEYTPVGLATTMISFYFDQMKALNNKLLERVRSLNQNVNQANPTPWLNGLSAKLPYVNTNGNYEKLNNYFTFLITKVLWPKVGTEDTNVSEEKSKLKTKTEDVNKIREKILNNIDSKLKTFVQKLKPTLAPRPAYSNVILLNINNDKVWSAGANWSLAVLLDPKKVNPLSFMLLKQMFDQNSLFKKAKTLFENIQNKAKTSGSGKSGTTTNDDADALSKVIGNYYYNTWAKLTDKSIYGNLKDDKFDDLFKLAFDSSINEKSFNVDYKAVIEHYRFIYTLEWLVDKNLKNFKDLLKANLKFGEIAFIAYKNTETQNFSNPQGIFGSYFNYENETNAAKSATQIIDPNSFFYKTTTKPEAKTTQSANTAVMVQNTQMNNQQTNSYGFTGLSTSSGSMLGAATQQAILDQITKTSLQQYGSQADLKKIIGETKNQLLLDRIANQLIALKPNTSGNSGTQKTIAAYFQTDAVGNPTLDFKAKQKLLLDVLDQYKDFFGNNAQAVQRDSGKSGTGNYLTYTDGSDKITYLQFSYKDIDGLSLSSSNGTSSKFASDVVAALLLFQAAYKGTQQLALSSINKPQLPIGDKRIKTGIDLLK" }, "paralog": false, "identity": 0.9954853273137697, "score": 862.0, "evalue": 4.56e-315, "description": "Internal stop codon at 533960, 534182. Nonsense mutation around 533960, 534182.", "psc": { "uniref90_id": "UniRef90_P75338", "product": "Uncharacterized protein MPN_440", "uniref50_id": "UniRef50_P75338", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75338", "UniRef:UniRef50_P75338" ] }, "pscc": { "uniref50_id": "UniRef50_P75338", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75338" ], "product": "Uncharacterized protein MPN_440" } }, "id": "APKECPOBEN_960", "locus": "GCA002090215_00944" }, { "type": "cds", "sequence": "contig_1", "start": 533958, "stop": 534176, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGCAGTTTTACTAGATCCGAAAAAGGTTAACCCGCTTTCGTTTATGTTGCTCAAACAAATGTTTGATCAAAACAGTTTGTTTAAAAAAGCAAAGACTTTATTCGAAAACATTCAAAATAAAGCAAAAACTAGTGGAAGTGGTAAAAGTGGTACAACCACCAACGATGATGCCGATGCTTTGAGCAAAGTCATTGGCAACTATTACTACAACACTTGA", "aa": "MAVLLDPKKVNPLSFMLLKQMFDQNSLFKKAKTLFENIQNKAKTSGSGKSGTTTNDDADALSKVIGNYYYNT", "aa_hexdigest": "db33fac5edd172cc0438b4f386071f20", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7995.079200000001, "isoelectric_point": 9.477771186828615 }, "id": "APKECPOBEN_961", "locus": "GCA002090215_00945" }, { "type": "cds", "sequence": "contig_1", "start": 534180, "stop": 534401, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGGCACTGAGGATACTAATGTCAGTGAAGAAAAAAGCAAACTCAAAACTAAAACTGAAGATGTTAACAAGATTAGGGAAAAGATTTTGAACAACATCGACAGTAAGCTCAAAACTTTTGTCCAAAAACTCAAACCTACCTTAGCACCCCGACCAGCTTACAGTAACGTAATCTTGTTAAACATTAACAATGATAAGGTGTGGTCTGCTGGCGCTAATTGA", "aa": "MGTEDTNVSEEKSKLKTKTEDVNKIREKILNNIDSKLKTFVQKLKPTLAPRPAYSNVILLNINNDKVWSAGAN", "aa_hexdigest": "ffd08ece77589e5777c47a77399b7a75", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8214.322999999999, "isoelectric_point": 9.522189903259278 }, "id": "APKECPOBEN_962", "locus": "GCA002090215_00946" }, { "type": "cds", "sequence": "contig_1", "start": 534408, "stop": 534647, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAGTGAATACACCCCGGTTGGTTTAGCTACCACCATGATTAGCTTTTATTTTGACCAAATGAAAGCTCTCAACAATAAGCTGTTAGAACGAGTACGGAGCTTAAACCAAAATGTTAACCAAGCCAATCCTACCCCTTGGTTAAATGGGTTATCGGCTAAACTACCTTACGTTAACACTAACGGTAATTACGAAAAGTTAAACAACTACTTTACCTTTTTAATTACCAAAGTTTTGTGA", "aa": "MSEYTPVGLATTMISFYFDQMKALNNKLLERVRSLNQNVNQANPTPWLNGLSAKLPYVNTNGNYEKLNNYFTFLITKVL", "aa_hexdigest": "2dbdcca73d4f0c7418938f98707c8bef", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9078.322099999998, "isoelectric_point": 9.397701454162597 }, "id": "APKECPOBEN_963", "locus": "GCA002090215_00947" }, { "type": "cds", "sequence": "contig_1", "start": 534648, "stop": 535040, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAACAGCAAGGAGAAACTAAAGATCAATATAACACTTTTGGCCTGAGACTTGTGCGTAACAGTGTTGGCGTATCAGTTTTAGGACTTGATGGTTTTGTTAAATTCATAAAAGGCGGTAGCGGAGGTAGCAATGGCGGTTCTAGTAGTGCCAAAAAGATTGATAAAGAAGAGCAAAAGAAATTCTTAAAGTTCCGTGCTTTTCAAGCCAAAATTGGCACATTTTATAACACTAACTTTGCCTTTAGTTTTCCCCTAAACGAAACTTTAAAAGGTTGGTTTGACAAACACCGCGGATTGATCTTAGCGAACGCCTTAGTTAAAGTTACTTTAGACACAAAGGAAAAAGCAAGTAAGGCCTTAGTGGATGCTTTTAGTTCTTATAAAAACTGA", "aa": "MQQQGETKDQYNTFGLRLVRNSVGVSVLGLDGFVKFIKGGSGGSNGGSSSAKKIDKEEQKKFLKFRAFQAKIGTFYNTNFAFSFPLNETLKGWFDKHRGLILANALVKVTLDTKEKASKALVDAFSSYKN", "aa_hexdigest": "526b1f0da89f6d803e10eacb4e5892cd", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14354.209699999976, "isoelectric_point": 9.910160636901853 }, "id": "APKECPOBEN_964", "locus": "GCA002090215_00948" }, { "type": "cds", "sequence": "contig_1", "start": 535047, "stop": 535571, "strand": "-", "frame": 2, "gene": null, "product": "DUF3713 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W962", "UniRef:UniRef90_A0AB38W962" ], "nt": "GTGGATGCCACCAACATTGTCGATCAATTAGAGGCAGCTGATTTAGCCGCTGTGCTCAACATTAAACTAGACCGACTGGGTCAAGAGAAGGATGAGCTACTCGAATTTAAGGAGCTCAAAGATGACCTTAACAACGTGATTGTGATTGATAAGAGTAGTGATGCACAAAATGTGAGCAAAACTAATTCTTCTTTTTATGAGGATAATCAAAACTCTGGAACCACAAATACCGATCCCATTGCCCAAGCTTTAGATGACATCTTTGCCACAAAAGTGGAACAAAAAACTAATCTAGGCAAGTTAGCAGAACAAATTAAAAAGGCAGCGGAAATTAAAACGCAAACCCCACAAGCAAGAGGCACTGTTGTTTTGCGAAATAAATCGCAAACTCAACAAAAGAACAGTTATGTTCTATTAGATGCTGGTATTCCTACCTTTAAACAGCAAGTCAGCACCCAGGGTCAAGGTTCAGTGAGTCTTCATAACGGTACCTTAATTTCTTTAAACACATCTACAGTTGCCTAA", "aa": "MDATNIVDQLEAADLAAVLNIKLDRLGQEKDELLEFKELKDDLNNVIVIDKSSDAQNVSKTNSSFYEDNQNSGTTNTDPIAQALDDIFATKVEQKTNLGKLAEQIKKAAEIKTQTPQARGTVVLRNKSQTQQKNSYVLLDAGIPTFKQQVSTQGQGSVSLHNGTLISLNTSTVA", "aa_hexdigest": "212cef7e6e1e5ce20ca0e06747c83c69", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 535047, "stop": 535816, "observations": { "deletion": [ 535666 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB38W962", "query_cov": 1.0, "subject_cov": 0.6796875, "identity": 0.983, "score": 313.0, "evalue": 2.39e-106, "gene_start": 1, "gene_end": 174, "reference_start": 83, "reference_end": 256, "reference_sequence": "MPFRFLLQRMKLLGVKMSAINAENNWLKVKKRLKKSQSNNKMAVKQKEFKKEGLTKYVRGTDRGQVIDFPLSLSDTNETKKVVDATNIVDQLEAADLAAVLNIKLDRLGQEKDELLEFKELKDDLNNVIVIDKSSDAQNVSKTNSSFYEDNQNSGTTNTDPIAQALDDIFATNVEQKTNLGKLAEQIKKTAEIKTQTPQARGTVVLRNKSQTQQKNSYVLLDAGIPTFKQQVSTQGQGSVSLHNGTLISLNTSTVA" }, "paralog": false, "identity": 0.9921875, "score": 483.0, "evalue": 8.08e-176, "description": "Frameshift due to deletion around 535666.", "psc": { "uniref90_id": "UniRef90_A0AB38W962", "product": "Protein of unknown function (DUF3713)", "uniref50_id": "UniRef50_A0AB38W962", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB38W962", "UniRef:UniRef50_A0AB38W962" ] }, "pscc": { "uniref50_id": "UniRef50_A0AB38W962", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W962" ] } }, "id": "APKECPOBEN_965", "locus": "GCA002090215_00949" }, { "type": "cds", "sequence": "contig_1", "start": 535619, "stop": 535732, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAAGAAGCGATTGAAGAAAAGCCAAAGCAACAACAAGATGGCAGTCAAACAAAAGGAGTTCAAAAAGAAGGCCTTACCAAGTATGTACGGGGAACCGACAGAGGCCAAGTAA", "aa": "MKKRLKKSQSNNKMAVKQKEFKKKALPSMYGEPTEAK", "aa_hexdigest": "0b74f29e3bce86cae85ff832bdcd0848", "start_type": "GTG", "rbs_motif": "AAAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4312.134300000001, "isoelectric_point": 10.297937965393064 }, "id": "APKECPOBEN_966", "locus": "GCA002090215_00950" }, { "type": "cds", "sequence": "contig_1", "start": 535749, "stop": 536150, "strand": "-", "frame": 2, "gene": null, "product": "Lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB33HU13", "UniRef:UniRef90_A0AB33HU13" ], "nt": "TTGTTTGAACAGCTCATTAACGATTTTGCTAGTCGGGTCTTTCAAAAGAACTACCTCGCTTATAAGGATGGTAATAAGGTATCATCAGGTCCTTTCTTATTTGACGAAATTTCTAAATCGAGCAATTGGGACAAAATTGTCTTTGATGCGGGCCAGTTTCCCGAAATCAATGATGCCTTCTTTGCCCAGATTCAAAGCGAAGTTTTCGATCAATGGGCGGAATACACCGATCCCACTGTTATTAGTTTCACTACCTTAAAGTACTCGGCTCCTAACCAGGGATTAGGGCAAATCTATAACCAGGACAAACTAAAGGATAAATTAACCCCTTCCTATGCCTTTCCGTTTTTTGCTGCAGCGGATGAAATTGCTGGGAGTCAAAATGTCGGCAATAAACGCTGA", "aa": "MFEQLINDFASRVFQKNYLAYKDGNKVSSGPFLFDEISKSSNWDKIVFDAGQFPEINDAFFAQIQSEVFDQWAEYTDPTVISFTTLKYSAPNQGLGQIYNQDKLKDKLTPSYAFPFFAAADEIAGSQNVGNKR", "aa_hexdigest": "d977642a34b4ae1a4b0f0c6075d6a4b2", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 535588, "stop": 536210, "observations": { "deletion": [ 535667 ], "mutation": [ 535751, 536165 ], "stop": [ 535616, 535751, 536165 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB33HU13", "query_cov": 1.0, "subject_cov": 0.6394230769230769, "identity": 0.985, "score": 271.0, "evalue": 3.45e-91, "gene_start": 1, "gene_end": 133, "reference_start": 22, "reference_end": 154, "reference_sequence": "MSNKTCWIAPGGGLETWKAQKLFEQLISDFASRVFQKNYLAYKDGNKVSSGPFLFDEISKSSNWDKIVFDAGQFPEINDAFFAQIQSEVFDQWAEYTDPTVISFTTLKYSAPNQGLGQIYNQDKLKDKLTPSYAFPFFAAADEIAGSQNVGNKRWKQLVKGEEAIEEKPKQQQDGSQTKGVQKRRPYQVCTGNRQRPSNWLSVKLIRY" }, "paralog": false, "identity": 0.9711538461538461, "score": 398.0, "evalue": 1.29e-143, "description": "Internal stop codon at 535616, 535751, 536165. Frameshift due to deletion around 535667. Nonsense mutation around 535751, 536165.", "psc": { "uniref90_id": "UniRef90_A0AB33HU13", "product": "Lipoprotein", "uniref50_id": "UniRef50_A0AB33HU13", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB33HU13", "UniRef:UniRef50_A0AB33HU13" ] }, "pscc": { "uniref50_id": "UniRef50_A0AB33HU13", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB33HU13" ], "product": "Lipoprotein" } }, "id": "APKECPOBEN_967", "locus": "GCA002090215_00951" }, { "type": "cds", "sequence": "contig_1", "start": 536143, "stop": 536343, "strand": "-", "frame": 1, "gene": null, "product": "putative lipoprotein MPN_442", "db_xrefs": [ "GO:0098552", "SO:0001217", "UniRef:UniRef50_P75336", "UniRef:UniRef90_P75336" ], "nt": "TTGACAGCTTGGTATGAAAATAACCAAGACAAACAGGTCACCCAGTTCTTTAAAGACACCAAAAAGAGTGTTGATGAGCAATACAACCAAGCCGTAGATAAGGTGGTATCGGCTAGCCGAAACAAAAACCTCTTTGTCCAACAAGACTTGCTGGATAGCGCCGGGGGGGTTAGAAACTTGAAAAGCCCAGAAGTTGTTTGA", "aa": "MTAWYENNQDKQVTQFFKDTKKSVDEQYNQAVDKVVSASRNKNLFVQQDLLDSAGGVRNLKSPEVV", "aa_hexdigest": "5663a320d04936ab63c3a0bb6b06c223", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 536131, "stop": 536583, "observations": { "mutation": [ 536145, 536553 ], "stop": [ 536145, 536553 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75336", "query_cov": 1.0151515151515151, "subject_cov": 0.44370860927152317, "identity": 0.97, "score": 122.0, "evalue": 2.76e-34, "gene_start": 1, "gene_end": 66, "reference_start": 81, "reference_end": 147, "reference_sequence": "MKKLLIKPQFWFLTLGGFISSSVILVACATPSNSALQTVFKARSNQFFNGEQGSLQNALATALKDPEANKQFVAAPLLKALTAWYENNQDKQVTQFFKDTKKSVDEQYNQAVDKVVSASRNKNLFVQQDLLDSAGGGVRNLKSPEVVWTAH" }, "paralog": false, "identity": 0.9801324503311258, "score": 282.0, "evalue": 1.26e-99, "description": "Internal stop codon at 536145, 536553. Nonsense mutation around 536145, 536553.", "psc": { "uniref90_id": "UniRef90_P75336", "product": "Uncharacterized lipoprotein MPN_442", "uniref50_id": "UniRef50_P75336", "go_ids": [ "GO:0098552" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75336", "UniRef:UniRef50_P75336", "GO:0098552" ] }, "pscc": { "uniref50_id": "UniRef50_P75336", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75336" ], "product": "Uncharacterized lipoprotein MPN_442" } }, "id": "APKECPOBEN_968", "locus": "GCA002090215_00952" }, { "type": "cds", "sequence": "contig_1", "start": 536580, "stop": 536912, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGATAGCTTTTACATCCACCGTTCTGGTAGAGTAGCGCGTTTTAACAGTTGGGGCAATTCCTATGTGTTTGATTTACCGCATAACCAACACAAATTAACTAAGTTTGCCCATAAAGGTATTTTGTTTAACAGTGTTCATTTAGAACGTGATGGTACTTTAAAGTTCCCCCAACTTAAACAAAGCAAACAAAAACCAGCAGTATCCAGTGAACTTAAAACTGCCATTAAGCGGATTAAGGCAGGATACAAGAAGGTCAAGCCTAACTATAAGAAGCGCCAAAAGCAGCAGATTGCGGAACTCTTTGCCAAGCGCAAACAACGCCGTTCATGA", "aa": "MDSFYIHRSGRVARFNSWGNSYVFDLPHNQHKLTKFAHKGILFNSVHLERDGTLKFPQLKQSKQKPAVSSELKTAIKRIKAGYKKVKPNYKKRQKQQIAELFAKRKQRRS", "aa_hexdigest": "78f6aee323532ab0e972ed95b1855ea9", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12969.92439999998, "isoelectric_point": 10.869127845764158 }, "id": "APKECPOBEN_969", "locus": "GCA002090215_00953" }, { "type": "cds", "sequence": "contig_1", "start": 536925, "stop": 537227, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00271.37" ], "nt": "GTGAAACACTTTCTGCTTAACCTCAACGGTCAAGAACCGTTTGCTGGTTTATTAGCTTTACTCAACTACCACCATAACGAACAGGTCTTGGTCTTTTGTTCCAACGCTAAATCACTCAAACAACTCACTAGTTTACTAGCCCACAACCAACTTAGCTTTAAAAGCCTCCATGGTCAACTAACGCCCAATGAGCGCAAACACATCTTTACTAGTGCCGCTAATAACACGGTGAGGGTACTAGTGGTATCTGACTTACTAGCACGCGGCATTGATCTGCCCCACTTTAGTGTGGTCATTTCATGA", "aa": "MKHFLLNLNGQEPFAGLLALLNYHHNEQVLVFCSNAKSLKQLTSLLAHNQLSFKSLHGQLTPNERKHIFTSAANNTVRVLVVSDLLARGIDLPHFSVVIS", "aa_hexdigest": "149f4a1276dfc73a3101094cbe2f739b", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00271.37", "name": "Helicase_C", "length": 84, "aa_cov": 0.83, "hmm_cov": 0.9880952380952381, "evalue": 2.8476168077949697e-19, "score": 64.88178253173828, "start": 17, "stop": 99 } ], "seq_stats": { "molecular_weight": 11140.772699999987, "isoelectric_point": 9.428775215148928 }, "id": "APKECPOBEN_970", "locus": "GCA002090215_00954" }, { "type": "cds", "sequence": "contig_1", "start": 537240, "stop": 537695, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00270.35" ], "nt": "GTGGTTGGCATAGCCGAAACTGGTAGTGGTAAAACATTCGCTTACCTCTTACCAGCGCTCAACCAAATTGATGTCACGCTCAACAAGCCCCAAGCGGTGGTGTTTGTACCAACTAAGGAGTTGAAGTGGCAAATTTTAACGATCCTCAACGACATTAAAGCCAGCTTTACTGGTTTAAAGGTCAGTGAGAACTTCAACAGTAATGCCCACCTGATTGTGAGCTTAATTGGCAAAGACATTATTCTCCACGAAACGATTAAGTACGTTGTCTTTGATGAGGTCGACATGTTTTTGGAGGACAGTTCCCAAGCAGAGTGGATTAACTGTGTCCAACTGTTTCAAAAACATAAACCTCACTTTGGCTTTTTTAGTGCCACGTTATTTAACGAACAGCTCCAGCAAATTCGCAAGCAAGTCGCACCTTTAACGGTCGTCAACCAAAAGAAACGTGCTTGA", "aa": "MVGIAETGSGKTFAYLLPALNQIDVTLNKPQAVVFVPTKELKWQILTILNDIKASFTGLKVSENFNSNAHLIVSLIGKDIILHETIKYVVFDEVDMFLEDSSQAEWINCVQLFQKHKPHFGFFSATLFNEQLQQIRKQVAPLTVVNQKKRA", "aa_hexdigest": "eb333483a33dc08916a189b699169f10", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00270.35", "name": "DEAD", "length": 152, "aa_cov": 0.8874172185430463, "hmm_cov": 0.9868421052631579, "evalue": 1.6027483418856377e-25, "score": 85.22837829589844, "start": 1, "stop": 134 } ], "seq_stats": { "molecular_weight": 17089.640799999994, "isoelectric_point": 7.862386894226074 }, "id": "APKECPOBEN_971", "locus": "GCA002090215_00955" }, { "type": "cds", "sequence": "contig_1", "start": 537804, "stop": 538826, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAACCGGGCTGCTGATGACACTTCAAGCTTCTTCTACACTAAGTCTAGTAGTAGTAGTACTGGCGCAGCACAAAGTAGTGCCACTGTTTTAAGGAGTTTAAATCAAGCTAGTGGCATGACCACAAAAACTGCCAAAAACCGCTATGGCTTTAGGGGTATTGTAACGAGTAGCACTAGTGGTAGTTTACCCGAAGCGGTAAGTAGACGTTTGTTTAAGCAGTTTGTTAACCAAACAGAAAAAGGCGTTAAAGTTGGTGGTCAAATGCTAATTACCACAGCTAAAAGTGGTAAAGCTACCTTAGTTTTAAAACAACAAGCTGACGACGCCGAATCCACCACCAACAACGCTTACAAAGGTGCCTTGTTTAGCTTTGGTTCGATGGATAACTTAAAGAACATCATCAACGGAATCCAAACGCAAACCGAGTTCGATGCGCTTTACAACCACTTAACTAGTGACCTCAACATTGATGTTACTGGGGTTGATAAAAACAAAACTTTAACGGAACAGAAAACTAGTTTAACGAGTTTTGTTGATTCTAACTTTAAGCAGAGTACTCAATCTGCACAGAGAGGCGATACAAGTGCACGCAGTGCAAGATCTGCAACAGTACAAATTAAGAAAACACAAGAAGATAACCAAAATACCAACTATAAAGACGTCTTTAGCCGCTTTGATGGTTACATTGGTGATAACAAGGTAGAGGAAAAGAACTACACTTCCTACCAATTCTTAAGTGACGGTGGCAAATACCACGCTACCTTTGTCAAACAGGTCAACCTTGATGATGTGGAGAAGATCGGCACTGATTCTCTAAAGCAAGAAGATTCCAGCAAAGACAAACGCCTCAACCTCTCCCTCGAAGAATTCCTCGCTGCAATTGCGCTAGAAGCCTTGGATCCTAATAATCAAACCCAAGCCATTAACGCCTTAATTAGTGGTAATAAGAAGGGTTTAGTTAAAGTAGGTGACTTTAGGATCTTTAGTTCTATTAGTGCGCAATGGGTAAGACGTTTTTAA", "aa": "MNRAADDTSSFFYTKSSSSSTGAAQSSATVLRSLNQASGMTTKTAKNRYGFRGIVTSSTSGSLPEAVSRRLFKQFVNQTEKGVKVGGQMLITTAKSGKATLVLKQQADDAESTTNNAYKGALFSFGSMDNLKNIINGIQTQTEFDALYNHLTSDLNIDVTGVDKNKTLTEQKTSLTSFVDSNFKQSTQSAQRGDTSARSARSATVQIKKTQEDNQNTNYKDVFSRFDGYIGDNKVEEKNYTSYQFLSDGGKYHATFVKQVNLDDVEKIGTDSLKQEDSSKDKRLNLSLEEFLAAIALEALDPNNQTQAINALISGNKKGLVKVGDFRIFSSISAQWVRRF", "aa_hexdigest": "3fc4d34734e7cf695c4a134f3a18b1cd", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 37290.88300000004, "isoelectric_point": 9.271343421936034 }, "id": "APKECPOBEN_972", "locus": "GCA002090215_00956" }, { "type": "cds", "sequence": "contig_1", "start": 538848, "stop": 539057, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGATAGCGAAAAGAATAAAAATGATTCACAATCAACTTTATCCAGTACCGCTTCAAGCACAAGTAATACTGGCTTAATTCAGTTAAGATCAGTCGTTTCCCTAGCACAAAATCAAGCAGCTGGCCAAGGCGGTGATAATAATAGTGACATCACCCAAACCGAAGTCAAAAACCCGAACTTTGTGTTTGGTTCGAGTGTGTATGATTGA", "aa": "MDSEKNKNDSQSTLSSTASSTSNTGLIQLRSVVSLAQNQAAGQGGDNNSDITQTEVKNPNFVFGSSVYD", "aa_hexdigest": "c53e9fdcbed1ac14f7763e28c1f4e525", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7242.607900000003, "isoelectric_point": 4.4446615219116214 }, "id": "APKECPOBEN_973", "locus": "GCA002090215_00957" }, { "type": "cds", "sequence": "contig_1", "start": 539061, "stop": 539330, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGACTTATTTGAATCACAAGCCCAAAAATCAATCTTTGGTCATGTTGGTTCATCTGATAAAAATTCTTCAACTAAGACGGGCATCGAAGGTGTCTTGGACACACTGTACAGCTCGCTAAACTTAGAAGAGCGCTTAGATAGTGATGATGTAATTGATTATCTGTCTTACCTCTACACGGCCCATTGGTTGTTAAAGGACAACTTAAAGAACTATAAGCAAAGCTTACAGTCGAAGTTGTCGCGTACTTCCAATGCCTTTTTAGTTTGA", "aa": "MDLFESQAQKSIFGHVGSSDKNSSTKTGIEGVLDTLYSSLNLEERLDSDDVIDYLSYLYTAHWLLKDNLKNYKQSLQSKLSRTSNAFLV", "aa_hexdigest": "a4d773122e2f7ea61da954a7f99a45c4", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10083.09349999999, "isoelectric_point": 5.152136421203611 }, "id": "APKECPOBEN_974", "locus": "GCA002090215_00958" }, { "type": "cds", "sequence": "contig_1", "start": 539355, "stop": 541151, "strand": "-", "frame": 2, "gene": null, "product": "putative lipo MG309-like protein", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P47551", "UniRef:UniRef90_P75334" ], "nt": "GTGTACGCGGCCATCCAAGACAAACTGTTAGACCAGTTTATTACCAATGAAAACCCCAACTTGGTATCCCGGGTCGTGTTTACCAATGAAACTCCCGTTGACGGGTTTGATAATTACTTTAACACTAAAGTAATTCAAAGTCCAACCCCTTCCTACCAGTTTCAGGTCTTTAACAAATACAACCAACAGAGTGGTGGCACTAAGGGAGCGAATGGCTTTAACTTATTGGCTAGCAACCTCAAGAGTTACAAAAACGACCAGAGCAAAGGGATTGATATTCCCAATAAGTTTTCCAGTGATAGTGGTGGCAAATTGTTGTTAAAGGCGAGCGACATGTTCGACACCTTTGATCCGAGTTTTTCTGCTGCTTTCATTCAGGGCTATTTAGCACTGCAGAAAAAGTCGAAGGGCGCTGATAGTAAAGAAGTTGACAGTCTCATTAAGGATAAGAGTATTATTGAAAACTTTTTTGTTGACAATAATACACAGGCAGCCGCCGCTGCAAGGGCTGCGAGTTCATCATCTGAAGGAACTATTCAATTAAAAACTGCCAGTGATGGTGGTGGAACTACACAAAGTACCGTCCACAAAACTGACTTAGTCAAAATCTTTGGTGATAAAGATGTCTTTGCTGGTGAGTATAAACAACAAATAGGTAATACTAATGCTAATCAAACAGGTGGCGGAGGTTCTGGAGGCGGCGGTGGCACCTCTACCGGCAGTAGCACCGGATCTTCTACTGAAACTACTACTGGCAACAGTAGTAAGGCGGTTGTTGACTTAATTGAAGTTAAAAAAGATTCTAGTAGTCAACCAGACTACATCCTCTCCCGTGGTAAAGACGGCATCCACTTAATGGCCGTGGATGGTGGTAGTCATTATCTTACTGAAAGTGGTCGTGATGTCGCTAAACAAAAGAAATTCTTATTGTTCCGGGCTTTGCAAACCAAGTACGGTTTAGTGGATACCGACACCACATACGACTTTAAGTTATTTGATGAGGTCAAAAAGTACTTTGACACTAACCGGATCTTATTCTTATTTGAAGCTTTATTAGATCTTTCTAGTGATACAAACAACAAAGACAACTTCTTAAGCTATCCCCAGTTTAAGAAGTTTGCTGACAGTATTAAGTCAATTGAGAAGGATTTAAAAGAACTTGTTCAAGCCCACTATAAACAAGCAGTCTTTAACGAAACTGCAGTGGCGGAAAACAAGGTTACCTTAAAGCTAGCAGAACGTAACCAACCTTTTATTGACAACGAGCGCAATAACCAGATTGAACAGAACGGTTTGGCAGCTAAGTTACCTTACGAACAAGATGCTAAAACCGGTCACTATAATGACCTTGGTAACTATTACAAGGACATTATTGATAATGTTGATAAAAAAGGAACTAGTACTGTTAAAACAACCTCTTCCAATACAGGACAAACTAAAAACTTCTCTGAAGAAGTAGTTAGCAAGCTTAAAGATAATAAGAAGAAGGTAGAAGAAGCTGCTAAAAAACATGTAGAGGCACTCAAAGTCTTCACCATTCCTTCCCCACTCTACTCCCAGGTAATCTTAGTGCAAACTAAGTTGTCTTTTACCCCTGAAAGTACCTCTTTAGGACTTAACTTAGCTCTAAACAACTATTTAACCAGTACTGAGCTCCAAAACAGCATTAAGCTCAGTTACTTCCAGGAAGATGAAGCGTTTAAGAAGATTATTGATATTACGAATTTAACTTTTAGTCAACAAAGTGGTGGTACTGGTGGCACCAACGGTAACAACAACCTCACCGCTGACAACTGA", "aa": "MYAAIQDKLLDQFITNENPNLVSRVVFTNETPVDGFDNYFNTKVIQSPTPSYQFQVFNKYNQQSGGTKGANGFNLLASNLKSYKNDQSKGIDIPNKFSSDSGGKLLLKASDMFDTFDPSFSAAFIQGYLALQKKSKGADSKEVDSLIKDKSIIENFFVDNNTQAAAAARAASSSSEGTIQLKTASDGGGTTQSTVHKTDLVKIFGDKDVFAGEYKQQIGNTNANQTGGGGSGGGGGTSTGSSTGSSTETTTGNSSKAVVDLIEVKKDSSSQPDYILSRGKDGIHLMAVDGGSHYLTESGRDVAKQKKFLLFRALQTKYGLVDTDTTYDFKLFDEVKKYFDTNRILFLFEALLDLSSDTNNKDNFLSYPQFKKFADSIKSIEKDLKELVQAHYKQAVFNETAVAENKVTLKLAERNQPFIDNERNNQIEQNGLAAKLPYEQDAKTGHYNDLGNYYKDIIDNVDKKGTSTVKTTSSNTGQTKNFSEEVVSKLKDNKKKVEEAAKKHVEALKVFTIPSPLYSQVILVQTKLSFTPESTSLGLNLALNNYLTSTELQNSIKLSYFQEDEAFKKIIDITNLTFSQQSGGTGGTNGNNNLTADN", "aa_hexdigest": "f1e9e0681b464a51f7a3e1648265f54e", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 539052, "stop": 541451, "observations": { "mutation": [ 539063, 539357, 541361 ], "stop": [ 539063, 539357, 541361 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75334", "query_cov": 1.0, "subject_cov": 0.44165435745937964, "identity": 0.995, "score": 1064.0, "evalue": 0.0, "gene_start": 1, "gene_end": 598, "reference_start": 211, "reference_end": 808, "reference_sequence": "MHTFTRKVKWPFMFTAIGLTFGIVAVACAQPTASTVEGLFKPSSAFADRTDFSLSSILQKSLINRESFNQYLAMRLAPVLRTFYDDNYDTDIKERLNGFTADTDNAFVSQEQNLRNQFRENYLVHLQTDIFDNTGGNQAAWKLRDVNNKIIDDFISRIFAKNFVEYVQDGVGPLTKPTKSLIENTSNFKNIKLQPKFVNKNAKLKINNDAVYAAIQDKLLDQFITNENPNLVSRVVFTNETPVDGFDNYFNTKVIQSPTPSYQFQVFNKYNQQSGGTKGANGFNLLASNLKSYKNDQSKGIDIPNKFSSDSGGKLLLKASDMFDTFDPSFSAAFIQGYLALQKKSKGADSKEVDSLIKDKSIIENFFVDNNTQAAAAARAASSSSEGTIQLKTASDGGGTTQSTVHKTDLVKIFGDKDVFAGEYKQQIGNTNANQTGGGGSGGGGGTSTGSSTGSSTETTTGNSSKAVVDLIEVKKDSSSQPDYILSRGKDGIHLMAVDGGSHYLTEIGRDVAKQKEFLLFRALQTKYGLVDTDTTYDFKLFDEVKKYFDTNRILFLFEALLDLSSDTNNKDNFLSYPQFKKFADSIKSIEKDLKELVQAHYKQAVFNETAVAENKVTLKLAERNQPFIDNERNNQIEQNGLAAKLPYEQDAKTGHYNDLGNYYKDIIDNVDKKGTSTVKTTSSNTGQTKNFSEEVVSKLKDNKKKVEEAAKKHVEALKVFTIPSPLYSQVILVQTKLSFTPESTSLGLNLALNNYLTSTELQNSIKLSYFQEDEAFKKIIDITNLTFSQQSGGTGGTNGNNNLTADNWKIFKETYLLDLFESQAQKSIFGHVGSSDKNSSTKTGIEGVLDTLYSSLNLEERLDSDDVIDYLSYLYTAHWLLKDNLKNYKQSLQSKLSRTSNAFLVWSVDSEKNKNDSQSTLSSTASSTSNTGLIQLRSVVSLAQNQAAGQGGDNNSDITQTEVKNPNFVFGSSVYDWTNSKTPEVNRAADDTSSFFYTKSSSSTGAAQSSATVLRSLNQASGMTTKTAKNRYGFRGIVTSSTSGSLPEAVSRRLFKQFVNQTEKGVKVGGQMLITTAKSGKATLVLKQQADDAESTTNNAYKGALFSFGSMDNLKNIINGIQTQTEFDALYNHLTSDLNIDVTGVDKNKTLTEQKTSLTSFVDSNFKQSTQSAQRGDTSARSARSATVQIKKTQEDNQNTNYKDVFSRFDGYIGDNKVEEKNYTSYQFLSDGGKYHATFVKQVNLDDVEKIGTDSLKQEDSSKDKRLNLSLEEFLAAIALEALDPNNQTQAINALISGNKKGLVKVGDFRIFSSISAQWVRRFLNHYALHSTTPSVLTNEAHCWIFIYSTSCV" }, "paralog": false, "identity": 0.9937421777221527, "score": 1481.0, "evalue": 0.0, "description": "Internal stop codon at 539063, 539357, 541361. Nonsense mutation around 539063, 539357, 541361.", "psc": { "uniref90_id": "UniRef90_P75334", "product": "Uncharacterized lipoprotein MG309 homolog", "uniref50_id": "UniRef50_P47551", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75334", "UniRef:UniRef50_P47551", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47551", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47551" ], "product": "Uncharacterized lipoprotein MG309" } }, "id": "APKECPOBEN_975", "locus": "GCA002090215_00959" }, { "type": "cds", "sequence": "contig_1", "start": 541359, "stop": 541781, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCATACGTTTACGCGTAAAGTCAAGTGGCCCTTTATGTTCACCGCAATCGGGCTCACTTTTGGTATAGTCGCGGTTGCCTGTGCGCAGCCTACAGCCAGCACTGTGGAGGGGCTTTTTAAGCCCAGTAGTGCTTTTGCTGATCGCACTGACTTTAGTCTTAGCTCGATTCTGCAAAAGTCACTAATTAACCGCGAGAGTTTTAACCAGTATCTAGCAATGCGTTTAGCACCCGTTTTGCGCACCTTTTATGAGGACAATTATGATACTGATATTAAAGAGCGTTTAAACGGCTTTACCGCTGACACGGATAATGCGTTTGTCAGTCAAGAACAAAACCTACGGAACCAGTTTCGCGAAAACTATTTAGTGCACCTGCAAACTGATATCTTTGACAACACTGGCGGTAATCAAGCCGCTTGA", "aa": "MHTFTRKVKWPFMFTAIGLTFGIVAVACAQPTASTVEGLFKPSSAFADRTDFSLSSILQKSLINRESFNQYLAMRLAPVLRTFYEDNYDTDIKERLNGFTADTDNAFVSQEQNLRNQFRENYLVHLQTDIFDNTGGNQAA", "aa_hexdigest": "6a2b81dc80a0ddd17277eac1f9efb05d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15891.64569999998, "isoelectric_point": 5.610031318664551 }, "id": "APKECPOBEN_976", "locus": "GCA002090215_00960" }, { "type": "cds", "sequence": "contig_1", "start": 541836, "stop": 542465, "strand": "+", "frame": 3, "gene": "menH", "product": "Putative esterase/lipase 1", "db_xrefs": [ "COG:COG0596", "COG:HR", "EC:3.1.-.-", "GO:0016020", "GO:0052689", "SO:0001217", "UniRef:UniRef50_P75333", "UniRef:UniRef90_P75333" ], "nt": "ATGCGGCTCGAAATTGAAAACGGCTTGGAATTTGTGTGTGATCCCTTTTTAAATGAGCGCGGCAAAATCTTTTTTTTACACGCCTTTACCGGTAATATCACCAATAAATTAAGCTTTCGCACCCACTTTAAAGATTACAGTTTTTACGGGATTAACTTTCCCGGACACGGTAACAGTGTCATTCACAACCAAAGTGAATTAGACTTTAACTTCTGGATTAAGTTAGTGCAACAGTTCTTTAATAAATACCAATTAAAGAACGTGGTTTTATTTGGTCACTCGATTGGTGGCGGACTAGCGATTGCCCTCACCCAAGTTTTAACTAAGGAACAAATTAAAGGCATTATCCTCGAAGCGCCCCTAAACCCGGGGATTCGCGCTACCCCACCAAGTATTATTAGTGCCTTGGTACCTGACACCAACGAAGACTTTGAAGCGGTACAAAGGGCATTGATCTACAACATTGAACAACGCTTTGGGGCGAACTTTAAGGACTTTTGTGCTAAACAAAAACAGAAGATGATCCAAAAGTATGCACCCTTAAAGGTAATGTTGCAACCCGAACAAGCCGAACAACGGCTCCAGTTAATTGATGCGGCTTTTAAACGGTTAAGTTACCCAACCCTGTGA", "aa": "MRLEIENGLEFVCDPFLNERGKIFFLHAFTGNITNKLSFRTHFKDYSFYGINFPGHGNSVIHNQSELDFNFWIKLVQQFFNKYQLKNVVLFGHSIGGGLAIALTQVLTKEQIKGIILEAPLNPGIRATPPSIISALVPDTNEDFEAVQRALIYNIEQRFGANFKDFCAKQKQKMIQKYAPLKVMLQPEQAEQRLQLIDAAFKRLSYPTL", "aa_hexdigest": "cef98c80c29a8b88824f3c633db184bc", "start_type": "ATG", "rbs_motif": null, "genes": [ "menH" ], "truncated": "both", "pseudogene": { "start": 541776, "stop": 542645, "observations": { "mutation": [ 541827, 542463 ], "stop": [ 541827, 542463 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75333", "query_cov": 1.0, "subject_cov": 0.7231833910034602, "identity": 0.995, "score": 412.0, "evalue": 2.3e-144, "gene_start": 1, "gene_end": 209, "reference_start": 21, "reference_end": 229, "reference_sequence": "MHSRSKFGIILKFNHFLWPGMRLEIENGLEFVCDPFLNERGKILFLHAFTGNITNKLSFRTHFKDYSFYGINFPGHGNSVIHNQSELDFNFWIKLVQQFFNKYQLKNVVLFGHSIGGGLAIALTQVLTKEQIKGIILEAPLNPGIRATPPSIISALVPDTNEDFEAVQRALIYNIEQRFGANFKDFCAKQKQKMIQKYAPLKVMLQPEQAEQRLQLIDAAFKRLSYPTLWIHGQEDGIVRYLPSKAYLESLHNPLIELVGLSNTAHTTFFEQPQQFLQLVEQFLNKLNK" }, "paralog": false, "identity": 0.9896193771626297, "score": 575.0, "evalue": 6.08e-211, "description": "Internal stop codon at 541827, 542463. Nonsense mutation around 541827, 542463.", "psc": { "uniref90_id": "UniRef90_P75333", "gene": "MenH", "product": "Putative esterase/lipase 1", "ec_ids": [ "3.1.-.-" ], "uniref50_id": "UniRef50_P75333", "cog_id": "COG0596", "cog_category": "HR", "go_ids": [ "GO:0016020", "GO:0052689" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75333", "EC:3.1.-.-", "UniRef:UniRef50_P75333", "COG:COG0596", "COG:HR", "GO:0016020", "GO:0052689" ] }, "pscc": { "uniref50_id": "UniRef50_P75333", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75333" ], "product": "Putative esterase/lipase 1" } }, "id": "APKECPOBEN_977", "locus": "GCA002090215_00961" }, { "type": "cds", "sequence": "contig_1", "start": 542490, "stop": 542645, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCGTTACTTGCCCTCGAAGGCTTACCTGGAATCCTTGCACAATCCTTTAATTGAACTAGTGGGCTTATCCAACACGGCCCACACCACTTTCTTTGAGCAGCCCCAGCAGTTTCTCCAACTAGTGGAACAGTTTTTAAACAAGTTAAACAAATAA", "aa": "MRYLPSKAYLESLHNPLIELVGLSNTAHTTFFEQPQQFLQLVEQFLNKLNK", "aa_hexdigest": "3aa89d62881a95c9adeba8ff1679c381", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5990.8384, "isoelectric_point": 6.705716514587402 }, "id": "APKECPOBEN_978", "locus": "GCA002090215_00962" }, { "type": "cds", "sequence": "contig_1", "start": 542706, "stop": 543323, "strand": "-", "frame": 2, "gene": "rpsD", "product": "30S ribosomal protein S4", "db_xrefs": [ "COG:COG0522", "COG:J", "GO:0003735", "GO:0006412", "GO:0015935", "GO:0019843", "GO:0042274", "KEGG:K02986", "RefSeq:WP_010874802.1", "SO:0001217", "UniParc:UPI000013500D", "UniRef:UniRef100_P46775", "UniRef:UniRef50_P46775", "UniRef:UniRef90_P46775" ], "nt": "ATGAAATACACTGGTAGTATTTTCAAACGATCACGCCGCTTAGGTTTTTCGTTACTAGAAAACAACAAGGAGTTTTCGAAAGGGAAGAAGCGGAAAACGATTCCTGGACAACACGGGAACCGTTTCCGTAGTTCCACTATGTCTGGTTATGCACAACAGTTGCAAGAAAAGCAACGGATGCAATACATGTATGGGATTACTGATAAACAGTTTAGACGCTTGTTTAGACTGGTATTAAAACAACGGGGTAACTTAGCGGTTAACCTGTTCCGTGTTTTAGAGTCACGTTTAGACAACATTGTGTACCGCATGGGTTTTGCTCCAACCCGTCGCAGTGCGCGCCAATTAGTTAACCACGGCCACGTATTACTCAACGATCGTACCGTGGACACCCCTTCCATTATTCTCAATCCAGGGGATAAGGTACGCTTGAAGGCCAAAACAATTAAGATTCCAATTGTTAAGGCGGCGAGCGAAAGCGGTGTGGTCTCCCCGTTTGTGGAAACCAACAACAAGACCTTTGAAGGGACATACGTGCGCTTCCCAGAACGTAGTGAACTCCCTGCTGGTATTAACGAATCGTACGTGGTCGAATGGTACAAGCGTTTGGTTAAGTAG", "aa": "MKYTGSIFKRSRRLGFSLLENNKEFSKGKKRKTIPGQHGNRFRSSTMSGYAQQLQEKQRMQYMYGITDKQFRRLFRLVLKQRGNLAVNLFRVLESRLDNIVYRMGFAPTRRSARQLVNHGHVLLNDRTVDTPSIILNPGDKVRLKAKTIKIPIVKAASESGVVSPFVETNNKTFEGTYVRFPERSELPAGINESYVVEWYKRLVK", "aa_hexdigest": "8a9fa98fa0470c7da55eccdbcc2f8093", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013500D", "ncbi_nrp_id": "WP_010874802.1", "uniref100_id": "UniRef100_P46775", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013500D", "RefSeq:WP_010874802.1", "UniRef:UniRef100_P46775" ] }, "ips": { "uniref100_id": "UniRef100_P46775", "uniref90_id": "UniRef90_P46775" }, "psc": { "uniref90_id": "UniRef90_P46775", "gene": "rpsD", "product": "30S ribosomal protein S4", "uniref50_id": "UniRef50_P46775", "cog_id": "COG0522", "cog_category": "J", "kegg_orthology_id": "K02986", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015935", "GO:0019843", "GO:0042274" ] }, "pscc": { "uniref50_id": "UniRef50_P46775", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P46775" ], "product": "Small ribosomal subunit protein uS4" }, "genes": [ "rpsD" ], "id": "APKECPOBEN_979", "locus": "GCA002090215_00963" }, { "type": "cds", "sequence": "contig_1", "start": 543346, "stop": 544350, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGAAACCAAAGAGGCTTCATCAAATGTTGATGTGGTACAACAACCAGTCAAACCACTCATGCCAGTGATGGTAGAACAGCTCAGAACAACGGAGTTACAGCCAACTACTGAAATTAATCTGTTTGCCAACAGCGACATTAACAGCATTATTGCCGAGTTAAAGCAAGGTCGTTCTAACCCTGCCATTAACTTCGATGACATCTTTAAAATGTCAAGTTACCAAATGGTGGTCAAAAAGTCGTTTGTCCAAATTAGTGACTTTATTACTAATTCCAAAACCGACATTACCAACCGCTTCTTGTTAATCAAAAAGGAATTACAAGCCGAATTGACCCGCTTAATTGAAGAAAACGAACAGCTCAAAGCGGAGTTTTTAAACGCTAAAGACCTTTCGGTGTACCAAAAGGATGAACTGCTACGCTCTTTATCCAACGACTTTACGATTGCTCACCGTCCAAGTGACAGTTACGAACAATTGCAAAAGTCCGGTGAGTTAGTGCGCAACATTCAAAAAGCGATCTTGGAAAACGAAAGCAAGATTAAAAACATCCAGATTACGCTAAAAGAACTTAAAGCCGTTTATAAGCTCTGCAGTGATACTGTTTTAAACGGGATGGCGAAGCTAGACAGTGTGTTGCGCTTTAACAAGAAGGAAAAGGATCCTTTATTGTTAAACAGCATGGAAACATTGTCTAGCTTTGAGACTGAACCGCAAGCCATTATTGAAGATCTCTTGGACTTCTCCAGTAGCTTTGACAAGATGTCCAATGAACAATTGGATGAGTTTGTCTACCAAAACCTAGACAGTGGGTTAAACCTTGATTTAGATGGGTTTGATCATCAGTTAAGCAGCATGAACATCCACGGGTTAGAGCCATTAGATCCAATGAAGTTAGATGACTTTGATTTTGAAACTTTAACTCCAGATAAAACCTCTAACCTCTCTAGTATCTTGGATGATGAACTAATGGAAAACGGTGGGGATTTTAATCTTGATTATTAA", "aa": "METKEASSNVDVVQQPVKPLMPVMVEQLRTTELQPTTEINLFANSDINSIIAELKQGRSNPAINFDDIFKMSSYQMVVKKSFVQISDFITNSKTDITNRFLLIKKELQAELTRLIEENEQLKAEFLNAKDLSVYQKDELLRSLSNDFTIAHRPSDSYEQLQKSGELVRNIQKAILENESKIKNIQITLKELKAVYKLCSDTVLNGMAKLDSVLRFNKKEKDPLLLNSMETLSSFETEPQAIIEDLLDFSSSFDKMSNEQLDEFVYQNLDSGLNLDLDGFDHQLSSMNIHGLEPLDPMKLDDFDFETLTPDKTSNLSSILDDELMENGGDFNLDY", "aa_hexdigest": "0ff80898b9ddaa1a42b984d2a32d594b", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 38118.626200000086, "isoelectric_point": 4.484448814392089 }, "id": "APKECPOBEN_980", "locus": "GCA002090215_00964" }, { "type": "cds", "sequence": "contig_1", "start": 544507, "stop": 545904, "strand": "-", "frame": 1, "gene": "hmw1", "product": "Cytadherence high molecular weight protein 1", "db_xrefs": [ "GO:0005886", "GO:0020035", "GO:0033111", "GO:0042995", "SO:0001217", "UniRef:UniRef50_Q50365", "UniRef:UniRef90_Q50365" ], "nt": "TTGGAAGCAGATGTACCAGCACCAGAAGTTGCTGAACCTAGCTATGAGGTACAACCAGAGGTAGCTGCTGAGCCTGTTTACGATGTTCAACCAGAAGTAGCTGTAGAACCGGTGGGAGAAACTACTGCAACAGTAGAACCACAAGCAGTAGAAATTCAACCAGAAGTAGTTGTAGAACCAATTGTTGAATCACAACTAGAACAACCTGTGGAAGTTCAAGCTGAAATGGTTCAACCAGAAGTAGCTGTAGAGCCACAATTAGAAGTAAGTCTAGATCCAATTGGAGAAACTGCACCAATATTGGAACAAGTTGAACCACAAGCTGTACAAACCCAACCAGAGATTCCAGCTGAACAATCAGCAGTAGAGCTTCAACCAGAACCAGTTGCAGAAGTTCAATCTGAAATGGTTCAACCAGAAGCAGCTGCAGAGCCAGTTACAGAAGCACAACAAACTGAACCAACACCAGTTGTTGAAACTATTGCAGAGATTACACCTCAGGTAGTTACAGAGCCTGTAGTAGCTGTTGTAGAACATCAACCGGAAGCTGTTGCCGAGCCACTTCCAGTAGAACCAGCTGTGGCAGGTGTGTCTGAATTAATTCCAACGGAACAAGTTCAACCAGAAGTGGTTGTGGAATCGACTCCAGTTGCAGAAGTTCAATCTGAAATGGTTCAACCAGAAGTAGCTGTAGAACCGATTGTTGAACCACAACCAGAACAACCTGTGGAAGTCCAACCAGAAGTAATTACAACCCCGGAAGTAGCTTCAGTTTTAGAAGTTCAACCTGAAAATCCTGTTGTAGAAGTGGAACAAGTAGTTGAACCACAACCTGAAACCCCTGTAGAAGTTCAACCAGAACCAGTTGTTGAAACTGTTCAAGAAGCTGTGGCTGAACCAACCCAAGTAGTTGAACCACAACCACAAGCTGCACCTCAACCAGCGGTTTATGAATGGAATCTAACTCCAGAAGCTGCTCCTGTTGAACAACCAGAAGTAATTCCAGTAACAGTAGTAGAAAGCCAAGCTACCGCTACCGCAGAACCTCAACCAGCAGTAGCACCCGTTGCTGATATGGATTATGTCTTACATCTCACCGATACAGTAAAAAACCAACCACAGACTGCACCAGTACAACCAACTACCCCAATTAAAATTGAAGTGGCGGAATCAACTCCTACCGTTACAACCAGTCCAGTTGAACCAACAATTGCCCCACCACTGTTTGAAATTGAACTCAATAATACAACTTCATCTGACTTACCATTAGTTGAGGTTGTGGACTTTAAGCACAATCAACACGGTGCTGTCGGTACCCACAGCTTTGATGATTTTACCCCACCAGAAGTGGGTATGGAAAGCAAGACCCACTGTCACAGTAACAGTGAGGTTGTATGA", "aa": "MEADVPAPEVAEPSYEVQPEVAAEPVYDVQPEVAVEPVGETTATVEPQAVEIQPEVVVEPIVESQLEQPVEVQAEMVQPEVAVEPQLEVSLDPIGETAPILEQVEPQAVQTQPEIPAEQSAVELQPEPVAEVQSEMVQPEAAAEPVTEAQQTEPTPVVETIAEITPQVVTEPVVAVVEHQPEAVAEPLPVEPAVAGVSELIPTEQVQPEVVVESTPVAEVQSEMVQPEVAVEPIVEPQPEQPVEVQPEVITTPEVASVLEVQPENPVVEVEQVVEPQPETPVEVQPEPVVETVQEAVAEPTQVVEPQPQAAPQPAVYEWNLTPEAAPVEQPEVIPVTVVESQATATAEPQPAVAPVADMDYVLHLTDTVKNQPQTAPVQPTTPIKIEVAESTPTVTTSPVEPTIAPPLFEIELNNTTSSDLPLVEVVDFKHNQHGAVGTHSFDDFTPPEVGMESKTHCHSNSEVV", "aa_hexdigest": "e14740b0e6a41c4e7de0cb26a7b8abb0", "start_type": "TTG", "rbs_motif": null, "genes": [ "hmw1" ], "truncated": "both", "pseudogene": { "start": 544204, "stop": 546204, "observations": { "mutation": [ 544509, 546027 ], "stop": [ 544509, 546027 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q50365", "query_cov": 1.0, "subject_cov": 0.4567779960707269, "identity": 0.998, "score": 469.0, "evalue": 1.05e-152, "gene_start": 1, "gene_end": 465, "reference_start": 167, "reference_end": 631, "reference_sequence": "MKKSKEAVFEDKDYTEENPEQIFGNLYDGKLTVQDGKVKIAYDGDGNGYYIAFNSETGVYYDPYGDTEYDISVLFDANGNSFVFADAPTVEVLAGEQEQTEAEPDYLQYVGNEAYGYYDEAGEWVWSGYFEGDQWISTLPQTEAEEKQFGFEDNIETTPTASEDFGLEADVPAPEVAEPSYEVQPEVAAEPVYDVQPEVAVEPVGETTATVEPQAVEIQPEVVVEPIVESQLEQPVEVQAEMVQPEVAVEPQLEVSLDPIGETAPILEQVEPQAVQTQPEIPAEQSAVELQPEPVAEVQSEMVQPEAAAEPVTEAQQTEPTPVVETIAEITPQVVTEPVVAVVEHQPEAVAEPLPVEPAVAGVSELIPTEQVQPEVVVESTPVAEVQSEMVQPEVAVEPIVEPQPEQPVEVQPEVITTPEVASVLEVQPENPVVEVEQVVEPQPETPVEVQPEPVVETVQEAVAEPTQVVEPQPQAAPQPAVYEWNLTPEAAPVEQPEVIPVTVVESQATATAEPQPAVAPVADMDYVLHLTDTVKNQPQTAPVQPTTPIKIEVAESTPTVTTSPVEPTIAPPLFEIELNNTTSSDLPLVEVVDFKHNQHGAVGTHSFDDFTPPEVGMESKTHCHSNSEVVWRVSEPKTVPVPPAVSSINIQTVNRVVEPTISTPTTPVVESAPAIEIFVDTPPVETKEASSNVDVVQQPVKPLMPVMVEQLRTTELQPTTEINLFANSDINSIIAELKQGRSNPAINFDDIFKMSSYQMVVKKSFVQISDFITNSKTDITNRFLLIKKELQAELTRLIEENEQLKAEFLNAKDLSVYQKDELLRSLSNDFTIAHRPSDSYEQLQKSGELVRNIQKAILENESKIKNIQITLKELKAVYKLCSDTVLNGMAKLDSVLRFNKKEKDPLLLNSMETLSSFETEPQAIIEDLLDFSSSFDKMSNEQLDEFVYQNLDSGLNLDLDGFDHQLSSMNIHGLEPLDPMKLDDFDFETLTPDKTSNLSSILDDELMENGGDFNLDY" }, "paralog": false, "identity": 0.996996996996997, "score": 886.0, "evalue": 5.07e-318, "description": "Internal stop codon at 544509, 546027. Nonsense mutation around 544509, 546027.", "psc": { "uniref90_id": "UniRef90_Q50365", "gene": "hmw1", "product": "Cytadherence high molecular weight protein 1", "uniref50_id": "UniRef50_Q50365", "go_ids": [ "GO:0033111", "GO:0042995", "GO:0005886", "GO:0020035" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q50365", "UniRef:UniRef50_Q50365", "GO:0033111", "GO:0042995", "GO:0005886", "GO:0020035" ] }, "pscc": { "uniref50_id": "UniRef50_Q50365", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50365" ], "product": "Cytadherence high molecular weight protein 1" } }, "id": "APKECPOBEN_981", "locus": "GCA002090215_00965" }, { "type": "cds", "sequence": "contig_1", "start": 546025, "stop": 546402, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF16713.10" ], "nt": "ATGAAGAAAAGCAAAGAAGCAGTTTTTGAAGACAAGGATTACACTGAGGAAAATCCCGAACAGATCTTTGGTAATTTGTACGATGGTAAATTAACTGTTCAAGATGGCAAAGTCAAGATTGCCTATGACGGTGATGGCAATGGTTATTACATTGCCTTTAACAGTGAAACTGGAGTTTACTATGATCCTTATGGTGACACGGAATACGACATTAGCGTGTTGTTTGACGCCAATGGTAATTCGTTCGTGTTTGCTGATGCACCAACAGTGGAAGTTTTAGCGGGGGAACAGGAACAAACCGAAGCAGAACCGGATTACCTTCAGTATGTCGGTAATGAGGCTTATGGTTACTATGATGAAGCTGGGGAGTGGGTTTGA", "aa": "MKKSKEAVFEDKDYTEENPEQIFGNLYDGKLTVQDGKVKIAYDGDGNGYYIAFNSETGVYYDPYGDTEYDISVLFDANGNSFVFADAPTVEVLAGEQEQTEAEPDYLQYVGNEAYGYYDEAGEWV", "aa_hexdigest": "8edc51495a30a9ddb3d51ef33bff627a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF16713.10", "name": "EAGR_box", "length": 22, "aa_cov": 0.176, "hmm_cov": 1.0, "evalue": 9.33237067836645e-10, "score": 33.555137634277344, "start": 104, "stop": 125 } ], "seq_stats": { "molecular_weight": 14077.873, "isoelectric_point": 4.0500284194946286 }, "id": "APKECPOBEN_982", "locus": "GCA002090215_00966" }, { "type": "cds", "sequence": "contig_1", "start": 546466, "stop": 546747, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATCTTAAACCTCTACATCTTAATGATGGCCTTACTAATGACCTTAGTGGTATCATTGTTAATTGGCACTGTAGCCTCGATTGAATACCTCGTCTTTTTAAGTGGTAAACCAAGTAAGAACTTCGTTAAGTATGGTAGCTATTTCTTTTTAATGCCAAGGGTCTTGGTACAAGCGTTGTTAATGCCCTTGTACTTGGCCTTGTTTAAGCCCTTAATTAGGATTGCTGAAAATAATTTAAGAAATTATTTAAGGGTATACAACCTCTCATGGAAAAGATAA", "aa": "MILNLYILMMALLMTLVVSLLIGTVASIEYLVFLSGKPSKNFVKYGSYFFLMPRVLVQALLMPLYLALFKPLIRIAENNLRNYLRVYNLSWKR", "aa_hexdigest": "1fd5821ba3dfdad57af239fdb45e0bf3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10850.224399999986, "isoelectric_point": 10.136186790466308 }, "id": "APKECPOBEN_983", "locus": "GCA002090215_00967" }, { "type": "cds", "sequence": "contig_1", "start": 546778, "stop": 546921, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTACCTGACTGATGGCAATTTTCAAGCTGCTGGTAACCAGAGCTTTGGCCGGGGCTTTGATGTCAATGTCCACATCCTACAAGGCATTACGATGGCAGCCTTTATTAGCTTTTTTGTGGGGTTAGAAATCTTTTTGGGTTGA", "aa": "MYLTDGNFQAAGNQSFGRGFDVNVHILQGITMAAFISFFVGLEIFLG", "aa_hexdigest": "bb18af03e57aff4bd5780654a1f755b3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5115.793199999997, "isoelectric_point": 4.535092353820801 }, "id": "APKECPOBEN_984", "locus": "GCA002090215_00968" }, { "type": "cds", "sequence": "contig_1", "start": 546991, "stop": 547257, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCACTAAGCTTTATCTTTAATATTTTTTCCATTAATGTCACTAGTGTGCTCAAGGTGAGTTTCACAAGAATTCCCTTTGCCTTAATTGGTTGGATGTTTGGACCGGTGTGGGGCTTTACCTTTGGCGCCATTGCCGACACGATGGACTGGCTTACTAGGGGCTATACCTGGTTTTGGTTGTTTGCGATTCAAAAACCCATGTTCTGCTTTTTAGCGGGGCTGGTTAAGGGCGTTTATCAGGTACGCCAAAGTAGTACTAACTGA", "aa": "MALSFIFNIFSINVTSVLKVSFTRIPFALIGWMFGPVWGFTFGAIADTMDWLTRGYTWFWLFAIQKPMFCFLAGLVKGVYQVRQSSTN", "aa_hexdigest": "f28142e6e63ca6ca4e1b4291e79cfbc1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10104.854499999996, "isoelectric_point": 9.860197639465333 }, "id": "APKECPOBEN_985", "locus": "GCA002090215_00969" }, { "type": "cds", "sequence": "contig_1", "start": 547374, "stop": 548099, "strand": "-", "frame": 2, "gene": null, "product": "MPN449 family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50363", "UniRef:UniRef90_Q50363" ], "nt": "ATGTTGTTAATTGGAGCGGCCGCCATTGCTTACCTCGTGGTGGTAAACCTCTTGTTTTACAGGTATGTAAACCCTGACAGTAACCTAACTAAAGCGGTTACTGGTCAGAATAATACCGACAGGCCAGCGTTAGTTGATTTAAACGGTGGCATTAACCTCTTTTTCCCTGTTAGTGCTTCTACTTTAATTATCTTGATCTTCTTGGGTTTTGGTAGTACTTCCTTCCTTTTAGCCTTTCAAGGTAAGCCCTACAGTTTTGCTACTAGAAGTCAAACCTTTAAGGCAATGCACTTTCTCAAGCACCAGTTTGGGGTAACTGATTTTCCCAGGATCAATGACAACTACCGGTATAAGGTCAGAATTAAGTGGATCTTCTGGACAATCTTCTTGTTTGTGGCACTCAATGCCCTACCTGGGGGTAACATTATTACCGGTGGTATTTTAAACCCCTCTTTTCTGTTAAATGCCTTAAGGTCTAATGAATTAAAAATTAACAGTGAGACCTTTAAAACTATCTTTGTTGGTTTTTCCATATTTTACCTAGCGAGCATTATTCCCTTTGCCCTCATTAGTATTATTGCCTTTGTTTTGAGTCCTAAACCAAGTTCGGATCAAACAAACGAAGTGTTGAACCGTTACGTGCAAGAGGAAATGCAACAGCCATTTAAAACGGATTGTGATCCTAACAACGACAATGATTTAACACCCCCAGCTGTTTTTGGGTAA", "aa": "MLLIGAAAIAYLVVVNLLFYRYVNPDSNLTKAVTGQNNTDRPALVDLNGGINLFFPVSASTLIILIFLGFGSTSFLLAFQGKPYSFATRSQTFKAMHFLKHQFGVTDFPRINDNYRYKVRIKWIFWTIFLFVALNALPGGNIITGGILNPSFLLNALRSNELKINSETFKTIFVGFSIFYLASIIPFALISIIAFVLSPKPSSDQTNEVLNRYVQEEMQQPFKTDCDPNNDNDLTPPAVFG", "aa_hexdigest": "e77a50d83febfe58f6b787ac51b0f917", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 547374, "stop": 548399, "observations": { "mutation": [ 548126, 548135 ], "stop": [ 548126, 548135 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q50363", "query_cov": 1.0, "subject_cov": 0.5379464285714286, "identity": 0.996, "score": 469.0, "evalue": 6.59e-164, "gene_start": 1, "gene_end": 241, "reference_start": 208, "reference_end": 448, "reference_sequence": "MTFSDLLTKLQDNLDIVFNANALKERIQSDPAFAKTIREQLKLLYFLEQKQAKAKTKKKDFQPVFQNLEARFVSLGQTKLANTELNLKLDLTDATDLANYLPIAVCNLFNRDLNSFSKLSSVQPTAVEKTTNGANKPTVTIDLNQPRIHTTGTVSPELENFVNESLEARARQRAFMRVTSERMVGKIFEFQFKSQWIKWAQLAIFISMLLIGAAAIAYLVVVNLLFYRYVNPDSNLTKAVTGQNNTDRPALVDLNGGINLFFPVSASTLITLIFLGFGSTSFLLAFQGKPYSFATRSQTFKAMHFLKHQFGVTDFPRINDNYRYKVRIKWIFWTIFLFVALNALPGGNIITGGILNPSFLLNALRSNELKINSETFKTIFVGFSIFYLASIIPFALISIIAFVLSPKPSSDQTNEVLNRYVQEEMQQPFKTDCDPNNDNDLTPPAVFG" }, "paralog": false, "identity": 0.9912023460410557, "score": 664.0, "evalue": 3.62e-243, "description": "Internal stop codon at 548126, 548135. Nonsense mutation around 548126, 548135.", "psc": { "uniref90_id": "UniRef90_Q50363", "product": "MPN449 family protein", "uniref50_id": "UniRef50_Q50363", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q50363", "UniRef:UniRef50_Q50363" ] }, "pscc": { "uniref50_id": "UniRef50_Q50363", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50363" ], "product": "Uncharacterized protein MG314 homolog" } }, "id": "APKECPOBEN_986", "locus": "GCA002090215_00970" }, { "type": "cds", "sequence": "contig_1", "start": 548133, "stop": 548720, "strand": "-", "frame": 2, "gene": null, "product": "MPN449 family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50363", "UniRef:UniRef90_Q50363" ], "nt": "ATGACTTTTAGCGATCTTTTAACCAAGTTACAAGACAATTTAGACATTGTTTTTAATGCCAATGCCCTCAAAGAGCGGATCCAAAGCGACCCGGCGTTTGCTAAAACAATTCGCGAACAGTTAAAGCTGTTGTACTTTTTAGAGCAAAAGCAAGCTAAGGCTAAAACCAAGAAAAAGGACTTTCAACCTGTTTTTCAAAACTTAGAAGCGCGCTTTGTTAGTTTAGGGCAAACCAAACTAGCGAACACCGAACTTAACCTCAAACTTGATTTAACTGATGCCACGGACTTGGCTAACTATTTACCAATTGCCGTGTGCAATCTCTTTAACCGTGATTTAAATAGCTTTAGTAAGTTAAGTAGCGTTCAACCAACAGCTGTTGAAAAAACAACCAATGGAGCTAATAAACCGACGGTTACAATTGATTTAAACCAACCACGGATACATACAACTGGTACTGTAAGCCCAGAGTTGGAAAACTTTGTCAACGAATCACTCGAAGCACGTGCACGCCAACGTGCCTTTATGCGGGTCACCTCGGAACGGATGGTAGGCAAAATCTTTGAATTCCAGTTTAAAAGCCAGTGA", "aa": "MTFSDLLTKLQDNLDIVFNANALKERIQSDPAFAKTIREQLKLLYFLEQKQAKAKTKKKDFQPVFQNLEARFVSLGQTKLANTELNLKLDLTDATDLANYLPIAVCNLFNRDLNSFSKLSSVQPTAVEKTTNGANKPTVTIDLNQPRIHTTGTVSPELENFVNESLEARARQRAFMRVTSERMVGKIFEFQFKSQ", "aa_hexdigest": "d7f164fbd3bcdda1858d2b919b7ec8c9", "start_type": "ATG", "rbs_motif": "AAAA", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 547830, "stop": 548720, "observations": { "mutation": [ 548126, 548135 ], "stop": [ 548126, 548135 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q50363", "query_cov": 1.0, "subject_cov": 0.43526785714285715, "identity": 1.0, "score": 369.0, "evalue": 4.35e-125, "gene_start": 1, "gene_end": 195, "reference_start": 1, "reference_end": 195, "reference_sequence": "MTFSDLLTKLQDNLDIVFNANALKERIQSDPAFAKTIREQLKLLYFLEQKQAKAKTKKKDFQPVFQNLEARFVSLGQTKLANTELNLKLDLTDATDLANYLPIAVCNLFNRDLNSFSKLSSVQPTAVEKTTNGANKPTVTIDLNQPRIHTTGTVSPELENFVNESLEARARQRAFMRVTSERMVGKIFEFQFKSQWIKWAQLAIFISMLLIGAAAIAYLVVVNLLFYRYVNPDSNLTKAVTGQNNTDRPALVDLNGGINLFFPVSASTLITLIFLGFGSTSFLLAFQGKPYSFATRSQTFKAMHFLKHQFGVTDFPRINDNYRYKVRIKWIFWTIFLFVALNALPGGNIITGGILNPSFLLNALRSNELKINSETFKTIFVGFSIFYLASIIPFALISIIAFVLSPKPSSDQTNEVLNRYVQEEMQQPFKTDCDPNNDNDLTPPAVFG" }, "paralog": false, "identity": 0.9898648648648649, "score": 562.0, "evalue": 1.23e-203, "description": "Internal stop codon at 548126, 548135. Nonsense mutation around 548126, 548135.", "psc": { "uniref90_id": "UniRef90_Q50363", "product": "MPN449 family protein", "uniref50_id": "UniRef50_Q50363", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q50363", "UniRef:UniRef50_Q50363" ] }, "pscc": { "uniref50_id": "UniRef50_Q50363", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50363" ], "product": "Uncharacterized protein MG314 homolog" } }, "id": "APKECPOBEN_987", "locus": "GCA002090215_00971" }, { "type": "cds", "sequence": "contig_1", "start": 548909, "stop": 549631, "strand": "-", "frame": 3, "gene": "holA", "product": "DNA polymerase III subunit delta", "db_xrefs": [ "COG:COG1466", "COG:L", "EC:2.7.7.7", "GO:0003677", "GO:0003887", "GO:0006260", "GO:0006261", "GO:0009360", "SO:0001217", "UniRef:UniRef50_Q50362", "UniRef:UniRef90_Q50362" ], "nt": "ATGACCGTAGTTTATGGTGCCGATATTGGGTTAATTCACCAGCAGTTAAACCAACTGCTCAACCCAGCCGCTTGTAAACAGGTCTGGTTTCAAGATGTTAATAAGCTGTATGATGTGGTGTTAAACCAGAATCTGTTTGCTGAGGACACTAAACCGATCCTCATTCACAACTGCAGTTTTTTGGAAAAGAACAACTTAACTAAGGCTGAATTACACTGCTTAAAAACACTTAAAGATACTGATGTGGTGGTCACCATTTACAGCGACAGTCCCGCTAATGCCCTCATTAATGACCGGGCAATTACCAAGTATGCTTGTAAACCAGTCACAGCTAAAACCATCCACCAGGTGATCAGTAAGGCCGCTAAGACACTCAAACTCAACTTAAACCCTGATTTAATTGACCACTTAGCCACGATTCTACCCTTTAACTTAGGGGTCATTGAACAGGAGTTGCGTAAGTTAACTCTACTTAGCCCGGCAGAATTACAGGACAAAAAAATGTTAGAAGCCGTTTTGTGTGATTACCAAACCAGTCAAATCTTACAATTAACTGACGCCATGGTGCGTTTGCAAACAGCTAAAGCCTTAAAGTTGATTGAACGCTTGTTTTTACCAAAGCAGCTCACACCACCGCAATTCCTAGAATTTTTAGCAAATGAACTACTTTTGGCCTTGATGGTCAAAGGCGTTAAACCACAAGCTGTTTTCCAACTACAGTGA", "aa": "MTVVYGADIGLIHQQLNQLLNPAACKQVWFQDVNKLYDVVLNQNLFAEDTKPILIHNCSFLEKNNLTKAELHCLKTLKDTDVVVTIYSDSPANALINDRAITKYACKPVTAKTIHQVISKAAKTLKLNLNPDLIDHLATILPFNLGVIEQELRKLTLLSPAELQDKKMLEAVLCDYQTSQILQLTDAMVRLQTAKALKLIERLFLPKQLTPPQFLEFLANELLLALMVKGVKPQAVFQLQ", "aa_hexdigest": "086fc53dabb39ca3579aac98dda307f0", "start_type": "ATG", "rbs_motif": null, "genes": [ "holA" ], "truncated": "5-prime", "pseudogene": { "start": 548732, "stop": 549676, "observations": { "mutation": [ 548860, 548911 ], "stop": [ 548860, 548911 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q50362", "query_cov": 1.0, "subject_cov": 0.7643312101910829, "identity": 1.0, "score": 462.0, "evalue": 4.44e-163, "gene_start": 1, "gene_end": 240, "reference_start": 16, "reference_end": 255, "reference_sequence": "MGCGACCILWVQSFTMTVVYGADIGLIHQQLNQLLNPAACKQVWFQDVNKLYDVVLNQNLFAEDTKPILIHNCSFLEKNNLTKAELHCLKTLKDTDVVVTIYSDSPANALINDRAITKYACKPVTAKTIHQVISKAAKTLKLNLNPDLIDHLATILPFNLGVIEQELRKLTLLSPAELQDKKMLEAVLCDYQTSQILQLTDAMVRLQTAKALKLIERLFLPKQLTPPQFLEFLANELLLALMVKGVKPQAVFQLQWNVNPFRLKAIQSQYRFWSTNQLTALINAIWQLDIKIKRNDGLAIHLLKHFVLRFFAQK" }, "paralog": false, "identity": 0.9936305732484076, "score": 615.0, "evalue": 1.06e-225, "description": "Internal stop codon at 548860, 548911. Nonsense mutation around 548860, 548911.", "psc": { "uniref90_id": "UniRef90_Q50362", "gene": "holA", "product": "DNA polymerase III subunit delta", "ec_ids": [ "2.7.7.7" ], "uniref50_id": "UniRef50_Q50362", "cog_id": "COG1466", "cog_category": "L", "go_ids": [ "GO:0003677", "GO:0003887", "GO:0006260", "GO:0006261", "GO:0009360" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q50362", "EC:2.7.7.7", "UniRef:UniRef50_Q50362", "COG:COG1466", "COG:L", "GO:0003677", "GO:0003887", "GO:0006260", "GO:0006261", "GO:0009360" ] }, "pscc": { "uniref50_id": "UniRef50_Q50362", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50362" ], "product": "Probable DNA polymerase III subunit delta" } }, "id": "APKECPOBEN_988", "locus": "GCA002090215_00972" }, { "type": "cds", "sequence": "contig_1", "start": 549819, "stop": 549953, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTAACTTCCACCTTAATCCTCATTGTGGTCAGTCCGATTACTTTGCACTTAAATCGAAAATTAAACGCTTTAAGTGTGTTTCATAACCTCTTGTTTACTCCCATAGCCTTGTTTTACTTCTGTGCTAGCTGA", "aa": "MLTSTLILIVVSPITLHLNRKLNALSVFHNLLFTPIALFYFCAS", "aa_hexdigest": "c80a687094b032b51a396136492493e0", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4946.955299999999, "isoelectric_point": 9.304415702819824 }, "id": "APKECPOBEN_989", "locus": "GCA002090215_00973" }, { "type": "cds", "sequence": "contig_1", "start": 549969, "stop": 550289, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATTAGTGGTTTTCACCTTAGTTTTTTGTTTGGTGTCATGGAGCGCTGGTTGTACAAGCGCTTTTACATCAATAAAATAACGGGTTTTGTAATGCTGTTGCTGTACCTCTTTTTGGTCGGATTTGCCTTTTCAGCTTTACGGGTTTTTGTCTCAAAGCTGTTACGGCAAATGTTTCCCAAGCAACTACCCGAAAATAATTTGGGCTTAACGGCGCTGTTAATCATCTTAATGAGTCCGGGAGCGTTACACAACTTTGGCTTTAACTTTTCCTTTTTAGCGTGCTTTGTGTTGTTTGCCATTAATAAGGTGAAGCTGTGA", "aa": "MISGFHLSFLFGVMERWLYKRFYINKITGFVMLLLYLFLVGFAFSALRVFVSKLLRQMFPKQLPENNLGLTALLIILMSPGALHNFGFNFSFLACFVLFAINKVKL", "aa_hexdigest": "0a5e240b53b4d9b5b8e822d756a33c53", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12265.821599999981, "isoelectric_point": 10.294843482971189 }, "id": "APKECPOBEN_990", "locus": "GCA002090215_00974" }, { "type": "cds", "sequence": "contig_1", "start": 550431, "stop": 550565, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATAGTTAACCTAATTAGTTTAACGGTACACTGGAAGTTTGGCTTACTGTTAATCAGTTGTAGTGCCGCTTTACTAGCATACTGTCAGTTAATGCCAGAGTTTCAACTAGCCAAACTAATGAAAGCGAACTGA", "aa": "MIVNLISLTVHWKFGLLLISCSAALLAYCQLMPEFQLAKLMKAN", "aa_hexdigest": "ac259c2c66f82dcac581e709e9b4dad9", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4938.056199999999, "isoelectric_point": 8.803689765930176 }, "id": "APKECPOBEN_991", "locus": "GCA002090215_00975" }, { "type": "cds", "sequence": "contig_1", "start": 550704, "stop": 550970, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTGTAAACTACCGCAACCCAGCGAGTTTGAACTTCGACAGCCTTAACAGCTTTGGCGCTTACCGCAGTCCAAGTAGCTTTTACCCACTTAGAAGACCATTGGAATTGTCGAGCTTAAGGCGTAACCGCAGTTCCTTTTTCAACACGCACCGCTTTGATCTAGGGAGCAATTACACCAGTTTCACCCCGCGTTACCGCTCACCGTTACGTGGTGGTTTGTCTCAACGCTTTCCGCTCCGTTCCAGCTGGAGTAAAGAGTTTTAA", "aa": "MAVNYRNPASLNFDSLNSFGAYRSPSSFYPLRRPLELSSLRRNRSSFFNTHRFDLGSNYTSFTPRYRSPLRGGLSQRFPLRSSWSKEF", "aa_hexdigest": "b4490d7ee4ccee62d3db0bd631e5022c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10322.40539999999, "isoelectric_point": 11.510265922546388 }, "id": "APKECPOBEN_992", "locus": "GCA002090215_00976" }, { "type": "cds", "sequence": "contig_1", "start": 551037, "stop": 551273, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGAACCACAACCGTGTCAGTCAGCTTGTGTTAATAATGAAAGTGGTGCAATTACCATTCACACCACCAACCGTTCCTTATTGTTGGAAAAGCTAGCGAGTTTGGGTCACCTCCACGATGCTTCTACCCGTACGCCTTTACCGCACGAGCGCTACCAGTTAGCTCCACCGAGTGAATATGTGGCTACCAAGTACAACGAACCGCTGTTTAACCTTCCAGCAATCCGCAACAGTTGA", "aa": "MEPQPCQSACVNNESGAITIHTTNRSLLLEKLASLGHLHDASTRTPLPHERYQLAPPSEYVATKYNEPLFNLPAIRNS", "aa_hexdigest": "7a17150965b1ec7c28b936cacf25a9d2", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8654.654499999993, "isoelectric_point": 6.386338233947753 }, "id": "APKECPOBEN_993", "locus": "GCA002090215_00977" }, { "type": "cds", "sequence": "contig_1", "start": 551295, "stop": 552722, "strand": "-", "frame": 2, "gene": "hmw3", "product": "Cytadherence high molecular weight protein 3", "db_xrefs": [ "GO:0005886", "GO:0020035", "GO:0033111", "GO:0042995", "SO:0001217", "UniRef:UniRef50_Q50360", "UniRef:UniRef90_Q50360" ], "nt": "ATGACAGATAAAGAAAGAGCAAAGCTAGCGAAAGCGTATGGTAAGCTAGCACAAAAGATCCAAAAGAGCTATCCCGACATTAATGTGGTTTATGGTCGTGATGCTAAAAATAAGCTTCACGCCCTGTACCAGGATCCTGAAACCGGGAACATCTTTTCTTTGGAAAAAAGAAAACAGCTCCCAGCGGATTATCCTCTGTTCGAATTGGACAGTGATGAACCAATTAGCTTTGCCCCCAAGATTATTCCACTCACTGCCTTTGATGGCAACAACAATGAGGTAATAGTCCAATACGACCAGGTCAACAATACCTTTTACGACCAAGATGGCAATGTTTTGGATGTGAGTGGTTACCGTGATGGGGAAAACATTCCCCTAGTGGACTATTTAAACTATGGTGGTAGTACCGCAAGTGCAGATACCACTACAAGCGAACCATTGAGTGGTGAGGGCTATCCGGACATTGATGCTGGTTTACCAGTTGTGGATCCCGATGCTACACCCGAACAGCAAGCTGATCAACTGTTCGGACTCGATCCACTACCTCAAGCACCAGACGAATATCAGGATACTACAGCACCACCAGCTTATGATCAGACCTTTGATCAAGCAACATACGACCAGCAAGCGTATGACCAAAACTATGATCCGAACGCTTACTATGACCAGCAAGCTTACGATCAGTCCTTTGATCAACAAGCGTATGATCAAGCCTATGATGCTAATGCTTACAATACACAGAACTATGATCAAGCTCACGATCCGAACGCTTACTATGATTCCCAAGCTTACAGCGATCCTGATCAAGCTAGTGCTGTAGCACCAATAGAGGTAGCACCACTCCAACCAGAACCAGTTGCACCAGTAGTGGAGCCAACTGCTGTTCCTATTGTTGAATCTGCTCCAATAGTAGAGGTTACTCCAACAGTAGAACCTACCCCAACACCAGTGGTAGAGACCGCACCAGTAGTGGAAGCTCCTAAGGTAGTGGAACCTACCCCAACACCAGTGGTAGAAGCTACTCCTGCCCCTAAAGTAGAGCCTAAGGTAGTGGAACAACCACAACCAACACCTGTAACTGTTGAGGTTGACTCACCTAAAGTTGAAATACCGAAGGTAGTAACAGCAAAAGTTGCACTCCAAGTGGCCCAACCAACCCCTGTTCCAGCTGTACCTAAAGTAGCACCACAACCAACCCCTGCTCCAGTAGTTGTCCAACCAACTGCTGTAGTTCAACCAGTTGTTAAGGCAGAACCGAAGGTAGTAACACCAACGCCAGCACCACAGGTAGTAGTTACTCCTCAAGTAGCTACACCTAAAGTGACACCTAAGGTAGTCCAAACAACTCCTGCGGTTCCACCAGTTGTAGTTCAACCAGAAGTGGTAGTCCAACCAATTATCCGTCCAACACAACCCGAACCGGAATGA", "aa": "MTDKERAKLAKAYGKLAQKIQKSYPDINVVYGRDAKNKLHALYQDPETGNIFSLEKRKQLPADYPLFELDSDEPISFAPKIIPLTAFDGNNNEVIVQYDQVNNTFYDQDGNVLDVSGYRDGENIPLVDYLNYGGSTASADTTTSEPLSGEGYPDIDAGLPVVDPDATPEQQADQLFGLDPLPQAPDEYQDTTAPPAYDQTFDQATYDQQAYDQNYDPNAYYDQQAYDQSFDQQAYDQAYDANAYNTQNYDQAHDPNAYYDSQAYSDPDQASAVAPIEVAPLQPEPVAPVVEPTAVPIVESAPIVEVTPTVEPTPTPVVETAPVVEAPKVVEPTPTPVVEATPAPKVEPKVVEQPQPTPVTVEVDSPKVEIPKVVTAKVALQVAQPTPVPAVPKVAPQPTPAPVVVQPTAVVQPVVKAEPKVVTPTPAPQVVVTPQVATPKVTPKVVQTTPAVPPVVVQPEVVVQPIIRPTQPEPE", "aa_hexdigest": "70100edd875ae52fafe40f6298207f4f", "start_type": "ATG", "rbs_motif": null, "genes": [ "hmw3" ], "truncated": "5-prime", "pseudogene": { "start": 550992, "stop": 552722, "observations": { "mutation": [ 551039, 551297 ], "stop": [ 551039, 551297 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q50360", "query_cov": 0.9136842105263158, "subject_cov": 0.6439169139465876, "identity": 0.975, "score": 551.0, "evalue": 5.62e-189, "gene_start": 1, "gene_end": 432, "reference_start": 1, "reference_end": 434, "reference_sequence": "MTDKERAKLAKAYGKLAQKIQKSYPDINVVYGRDAKNKLHALYQDPETGNIFSLEKRKQLPADYPLFELDSDEPISFAPKIIPLTAFDGNNNEVIVQYDQVNNTFYDQDGNVLDVSGYRDGENIPLVDYLNYGGSTASADTTTSEPLSGEGYPDIDAGLPVVDPDATPEQQADQLFGLDPLPQAPDEYQDTTAPPAYDQTFDQATYDQQAYDQNYDPNAYYDQQAYDQSFDQQAYDQAYDANAYNTQNYDQAHDPNAYYDSQAYSDPDQAXXSAVAPIEVAPLQPEPVAPVVEPTAVPIVESAPIVEVTPTVEPTPTPVVETAPVVEAPKVVEPTPTPVVEATPAPKVEPKVVEQPQPTPVTVEVDSPKVEIPKVVTAKVALQVAQPTPVPAVPKVAPQPTPAPVVVQPTAVVQPVVKAEPKVVTPTPAPQVVVTPQVATPKVTPKVVQTTPAVPPVVVQPEVVVQPIIRPTQPEPEWKPSPASVVEPQPCQSTCVNNESGAITIHTTNRSLLLEKLASLGHLHDASTRTPLPHERYQLAPPSEYVATKYNEPLFNLPAIRNSWARFTRPTVESTPIASRFTGVTPMAVNYRNPASLNFDSLNSFGAYRSPSSFYPLRRPLELSSLRRNRSSFFNTHRFDLGSNYTSFTPRYRSPLRGGLSQRFPLRSSWSKEF" }, "paralog": false, "identity": 0.9775086505190311, "score": 771.0, "evalue": 1.07e-277, "description": "Internal stop codon at 551039, 551297. Nonsense mutation around 551039, 551297.", "psc": { "uniref90_id": "UniRef90_Q50360", "gene": "hmw3", "product": "Cytadherence high molecular weight protein 3", "uniref50_id": "UniRef50_Q50360", "go_ids": [ "GO:0033111", "GO:0042995", "GO:0005886", "GO:0020035" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q50360", "UniRef:UniRef50_Q50360", "GO:0033111", "GO:0042995", "GO:0005886", "GO:0020035" ] }, "pscc": { "uniref50_id": "UniRef50_Q50360", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50360" ], "product": "Cytadherence high molecular weight protein 3" } }, "id": "APKECPOBEN_994", "locus": "GCA002090215_00978" }, { "type": "cds", "sequence": "contig_1", "start": 552736, "stop": 553512, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTAGTGCTGTTCAGCGCTTTAATAGTGCTTGCAACCTTAATTTTGGTACAGCACAACAATACCGAACTGACAGAAGTTAAGAGTGAATTGAGTCCCCTTAACGTTGTTTTACACGCAGAAGAGGATACAGTACAAATTCAGGGCAAGCCGATTACTGAGCAAGCATGGTTTATTCCTACAGTTGCTGGTTGCTTTGGTTTTAGTGCCCTAGCCATCATCTTGGGCCTTGCTATAGGACTGCCAATTGTGAAGCGCAAGGAAAAACGCTTATTGGAGGAAAAGGAACGCCAAGAACAGTTAGCGGAACAGCTACAACGCATTTCTGCCCAACAAGAAGAGCAACAAGCGTTAGAACAACAAGCAGCTGCTGAAGCCCATGCTGAAGCGGAAGTTGAACCAGCACCACAACCAGTACCAGTACCACCTCAACCCCAAGTCCAAATTAACTTCGGTCCCCGTACTGGTTTCCCACCTCAACCCGGTATGGCGCCTCGTCCAGGTATGCCGCCACACCCCGGTATGGCTCCAAGACCTGGTTTCCCACCTCAACCCGGTATGGCGCCTCGTCCAGGTATGCCGCCACACCCCGGTATGGCTCCAAGACCTGGTTTCCCACCTCAACCCGGTATGGCGCCTCGTCCAGGCATGCCGCCACACCCCGGTATGGCTCCAAGACCTGGTTTCCCACCTCAACCCGGTATGGCGCCTCGTCCCGGAATGCAACCACCACGTCCTGGCATGCCACCCCAACCCGGTTTTCCACCAAAACGCTAA", "aa": "MLVLFSALIVLATLILVQHNNTELTEVKSELSPLNVVLHAEEDTVQIQGKPITEQAWFIPTVAGCFGFSALAIILGLAIGLPIVKRKEKRLLEEKERQEQLAEQLQRISAQQEEQQALEQQAAAEAHAEAEVEPAPQPVPVPPQPQVQINFGPRTGFPPQPGMAPRPGMPPHPGMAPRPGFPPQPGMAPRPGMPPHPGMAPRPGFPPQPGMAPRPGMPPHPGMAPRPGFPPQPGMAPRPGMQPPRPGMPPQPGFPPKR", "aa_hexdigest": "c203741093ec73067aca9b91bbeb0168", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 27748.06859999991, "isoelectric_point": 7.987648963928223 }, "id": "APKECPOBEN_995", "locus": "GCA002090215_00979" }, { "type": "cds", "sequence": "contig_1", "start": 553581, "stop": 554162, "strand": "-", "frame": 2, "gene": null, "product": "MG319/MPN454 family protein", "db_xrefs": [ "GO:0005886", "RefSeq:WP_010874810.1", "SO:0001217", "UniParc:UPI0000139609", "UniRef:UniRef100_P75329", "UniRef:UniRef50_P47561", "UniRef:UniRef90_P75329" ], "nt": "ATGGGTTTCTTCAAATTGCTCTTTAAGCTAGCCTTCTTAGCGGTACTGACGGTAGCAATTGCTTACCTCTTTTTAGCAATCTTTTACTTTGGTACTGTTAGCCCATCCTTTAAACTAGCACAACCAATGGATGTGTTTAACCGTTTTTTCTCTAAGGAAGCGCTTGACACGGTTACTACTAGTGGTGTAGGCGCTACGGTAACTGCACCTAAAGCAGCTGCAGCATCACAGTCCCACGCCTTGATACTAGCAGCAGAACAAGTAGCTAATAATGCTAACAAGCTAGATCCCCGCTTTCCGATTGATGGTAGTTTGTTTAGCCAAATCCCAGGGTATGTCGACTTTTTAAAGAAACCCAATATCCCCGCCTTTTTAGAAAAGGTTGGTCCCTACCTTACTAAGTACATTATTCCGTTGGGCATGGCCTTAGTTTCAGGGCTTATAGGTGCCTTAATTATTAACTTTATCCTGTCCAAGATTACCCGTGCCATCATCAAAAAGCGCCGTAAAGCAAAACGGGCACAACAAAGTCGTGTTGATGATTACTACTATGATGAAGCACCCCGTCGCCGCCGCCGTTAG", "aa": "MGFFKLLFKLAFLAVLTVAIAYLFLAIFYFGTVSPSFKLAQPMDVFNRFFSKEALDTVTTSGVGATVTAPKAAAASQSHALILAAEQVANNANKLDPRFPIDGSLFSQIPGYVDFLKKPNIPAFLEKVGPYLTKYIIPLGMALVSGLIGALIINFILSKITRAIIKKRRKAKRAQQSRVDDYYYDEAPRRRRR", "aa_hexdigest": "a612f3c3079920ebcbea06e1929f8c55", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000139609", "ncbi_nrp_id": "WP_010874810.1", "uniref100_id": "UniRef100_P75329", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139609", "RefSeq:WP_010874810.1", "UniRef:UniRef100_P75329" ] }, "ips": { "uniref100_id": "UniRef100_P75329", "uniref90_id": "UniRef90_P75329" }, "psc": { "uniref90_id": "UniRef90_P75329", "product": "MG319/MPN454 family protein", "uniref50_id": "UniRef50_P47561", "go_ids": [ "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47561", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47561" ], "product": "Uncharacterized protein MG319" }, "genes": [], "id": "APKECPOBEN_996", "locus": "GCA002090215_00980" }, { "type": "cds", "sequence": "contig_1", "start": 554251, "stop": 554553, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTGAACTTTACGGTGTTCCGCGGTGGTCGCACTAATATCTTGGATTTACTGTTCCCCGGACTTTCGTTAATAGTTGGTGGGATCTTTTCCTTGTTATCGGTTTACTTTGTTTGTCTACAAATTCGCAACGAACAGGACTTGGTGAAACTGTATGAAAGTCAAGACCCAGCTTTAGCCAAAGCACAAATGTGGCGTAGTGCGCTCTTTTTTGGCGCTTGGTTGGTAAGTAGCTTCATGAACTTGGTCTACTTTATCCTGCGCATTATTTTGTTAACGCGTAATTTAAGCAGAGCTTTTTAA", "aa": "MLNFTVFRGGRTNILDLLFPGLSLIVGGIFSLLSVYFVCLQIRNEQDLVKLYESQDPALAKAQMWRSALFFGAWLVSSFMNLVYFILRIILLTRNLSRAF", "aa_hexdigest": "9c4b94a219939d2077e8cef7ce3052bb", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11483.538399999992, "isoelectric_point": 9.851107597351074 }, "id": "APKECPOBEN_997", "locus": "GCA002090215_00981" }, { "type": "cds", "sequence": "contig_1", "start": 554593, "stop": 555114, "strand": "-", "frame": 1, "gene": null, "product": "putative MG320-like protein", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P47562", "UniRef:UniRef90_P75328" ], "nt": "ATGATCAATCAAGCTAATAATCAGGTCAATTTTTCCGAGCAACAGTTTGTCCATCACAAGCGGTTCTCGGTCATTCGCTTGACCTTTTCTGTTGCCGCGATTGGCATTCTGTTTATCTTCTTAATTGGTTTTGGGGTACAACAGCTGTTAACCAACACTACTAGCCTTGGCACACTGGCTAGTGATATAAGAACGTTGGGTACGATTGCTTTTGTTGCTAGTTTGGTTTCCTTAATCCTTTACTTTGTCACGGCTTTTAAACTAAGGAACCGTAACACGACCTTAGCGTGGTTCTGGGGCTTAATTATTGCCGATGTGATTAGTTATGGGATTACATTAGGAGTATTGTTAACATTAGCCACCACACAATTACGTGAATTGATTGACTTTAAGTTTAGTGACATTGTCTTTGCCTTTTTAGGGGCAGCATTAGTGTATGGGACGGTCTGGGGCTTGAGTGCATTACCGAGCCAGCAACGCCGTTACCAACAAACCCAAACGCTGTTTCGTATCTTTATTTGA", "aa": "MINQANNQVNFSEQQFVHHKRFSVIRLTFSVAAIGILFIFLIGFGVQQLLTNTTSLGTLASDIRTLGTIAFVASLVSLILYFVTAFKLRNRNTTLAWFWGLIIADVISYGITLGVLLTLATTQLRELIDFKFSDIVFAFLGAALVYGTVWGLSALPSQQRRYQQTQTLFRIFI", "aa_hexdigest": "a3768fd76e5e46b3b01c7b669d5381d5", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 554290, "stop": 555114, "observations": { "mutation": [ 554595 ], "stop": [ 554595 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75328", "query_cov": 1.0, "subject_cov": 0.6027874564459931, "identity": 1.0, "score": 324.0, "evalue": 2.55e-110, "gene_start": 1, "gene_end": 173, "reference_start": 1, "reference_end": 173, "reference_sequence": "MINQANNQVNFSEQQFVHHKRFSVIRLTFSVAAIGILFIFLIGFGVQQLLTNTTSLGTLASDIRTLGTIAFVASLVSLILYFVTAFKLRNRNTTLAWFWGLIIADVISYGITLGVLLTLATTQLRELIDFKFSDIVFAFLGAALVYGTVWGLSALPSQQRRYQQTQTLFRIFIWAFFISIIASLLTFVLNFTVFRGGRTNILDLLFPGLSLIVGGIFSLLSVYFVCLQIRNEQDLVKLYESQDPALAKAQMWRSALFFGAWLVSSFMNLVYFILRIILLTRNLSRAF" }, "paralog": false, "identity": 0.9963503649635036, "score": 526.0, "evalue": 3.06e-192, "description": "Internal stop codon at 554595. Nonsense mutation around 554595.", "psc": { "uniref90_id": "UniRef90_P75328", "product": "Uncharacterized protein MG320 homolog", "uniref50_id": "UniRef50_P47562", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75328", "UniRef:UniRef50_P47562", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47562", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47562" ], "product": "Uncharacterized protein MG320" } }, "id": "APKECPOBEN_998", "locus": "GCA002090215_00982" }, { "type": "tRNA", "sequence": "contig_1", "start": 555184, "stop": 555274, "strand": "+", "gene": "trnS", "product": "tRNA-Ser(gga)", "amino_acid": "Ser", "anti_codon": "gga", "score": 69.6, "nt": "GGATACTTACCCAAGTGGCTGAAGGGGTAGGCTTGGAAAGCTTATAGATGGGTAACACCATGCGAGGGTTCGAATCCCTCAGTATCCGCCA", "db_xrefs": [ "SO:0000269" ], "anti_codon_pos": [ 555184, 555186 ], "id": "APKECPOBEN_13", "locus": "GCA002090215_00983" }, { "type": "tRNA", "sequence": "contig_1", "start": 555276, "stop": 555364, "strand": "+", "gene": "trnS", "product": "tRNA-Ser(cga)", "amino_acid": "Ser", "anti_codon": "cga", "score": 75.0, "nt": "GGAGACTTACCCAAGTGGTTTAAGGGGGCGCTCTCGAAAAGCGTTAGGTGTTTTTGCATGCGTGGGTTCGAATCCCACAGTCTCCGCCA", "db_xrefs": [ "SO:0000269" ], "anti_codon_pos": [ 555310, 555312 ], "id": "APKECPOBEN_14", "locus": "GCA002090215_00984" }, { "type": "cds", "sequence": "contig_1", "start": 555632, "stop": 556225, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGACGCAGCCACCCAATTGAACGATCAAGGTGGCGGTGATGTTATTACCTACTCCTCGGGCGCTCACATTGTACGTACGTTGTTGTTAGCTGCGAGTCAAAAAGATGTACATAGTAAATTCACACAAAAAATAGATCAACAAAATACAGCTTCAACCACATCTGATGTGACTGTTAAGAAAGCAGATTCATCTCAAGATAGCAGCAAGAGTAATACTGAAGAAGAAAAGTGGGATGACGTAACTTCCGCTGACCTCTTCAAAGATGACCCTTACGTACTAAAGAACTTTGGTGATGCTAAAGCACAAGCTGCACAAAGAAGCACCGGTTCTACAACTAGTGGTAACGGCACTCAAGCATCTTTAGAGTTCACCAAAAAGGCTTTAAGCTTGCTCAAATTCTTAGTGGATAATGGCATTCTAGACAAAGAAAAAGTAAAGGAAGCTATCAAAGATCCAAATAAGTACCTTGGAAAGCGTGACAAGATAGAGAACGGTACAAATAAACCAAGTAAAAACGAAGATTTCATTGGTTACGAGTTGAAAGATATCTATAAGAAAGCCGCCCAGCTCAACCGCTTTAACAGTATTTGA", "aa": "MDAATQLNDQGGGDVITYSSGAHIVRTLLLAASQKDVHSKFTQKIDQQNTASTTSDVTVKKADSSQDSSKSNTEEEKWDDVTSADLFKDDPYVLKNFGDAKAQAAQRSTGSTTSGNGTQASLEFTKKALSLLKFLVDNGILDKEKVKEAIKDPNKYLGKRDKIENGTNKPSKNEDFIGYELKDIYKKAAQLNRFNSI", "aa_hexdigest": "1ac39e1e171d2524d707ad0caed25d84", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 21591.63710000001, "isoelectric_point": 6.749141502380372 }, "id": "APKECPOBEN_999", "locus": "GCA002090215_00985" }, { "type": "cds", "sequence": "contig_1", "start": 556256, "stop": 556891, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCAAACTCCGGTGACCTGAACTATTCGATCGTACCTTTTGGCATCTTTAAGGAAAAGGGTAAAAATGGTGCAAGTGTTCAACAAAAAGCTGTCTCAACTACCGAAGGTTCAGATCTGATGAACGATTACTACTATAAGATCGAGAAGGAACAGCGCCTGGGCTTGATTCCACAGCGTGAAGGTAACTACGAAAAGAATAAAAACGTTTTGGAGAGTGCGACTGTTAAGATTAATTATTATAGCAGTAAAGCAACAAGTGGTCAGGCTGGAGCAGCTGCCTCTGCAGCGTTTGCTAAGAACAACAACACATCAGACAACACACAACAAAACCAAACCTCTTCAGTAGAAGCTAAGAGTGTAAACGTAACAAAGCACAGCTTTGTTCAAGCTTTAAAGAAGGTTGGTTTCTCTGGTAGCAATCCCCTCCACTTCAACATGAAGTTAGGGAACTCTTCGCTGTCCGCTAACGGGGTGGATTACTACAACGCCGTGAAGCAAGCCCTCACTGAATTGGGTACTGCTGACAACGGAGAAAAACTAATAGTACCAGAAATTATCTTAGGTGATGCCCAAGGACCAACTCGAAATGAGTGGTACATTGGGTTGTCCTCGGTGTTAGGTTTTAGTTCCTGA", "aa": "MPNSGDLNYSIVPFGIFKEKGKNGASVQQKAVSTTEGSDLMNDYYYKIEKEQRLGLIPQREGNYEKNKNVLESATVKINYYSSKATSGQAGAAASAAFAKNNNTSDNTQQNQTSSVEAKSVNVTKHSFVQALKKVGFSGSNPLHFNMKLGNSSLSANGVDYYNAVKQALTELGTADNGEKLIVPEIILGDAQGPTRNEWYIGLSSVLGFSS", "aa_hexdigest": "9040aa5d797a24641725794bf7631afc", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 22721.937099999992, "isoelectric_point": 8.58217639923096 }, "id": "APKECPOBEN_1000", "locus": "GCA002090215_00986" }, { "type": "cds", "sequence": "contig_1", "start": 556913, "stop": 557368, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGGGCCTTACTATGTCGAGAGTTTTAACCAGAGCCAGATTGTCTTTAAGCGTAACAGTGAGTATGATACTCATATAACCCCCAACTTACCAAAAACGCGGGAAGACAACGAAAAACCCATCCCAACGATGATTAACTACTTCCAACCAGGCGCTACCCCTGAAGTGTTTTACTCCAACTATATCGCTGGAGGATTATCTTCTGCGGAGGTGCCATACTCCCAACAAGAGGATGCTAGGTCACGATTTGCGGGTACGGGGGACTTACGCTGGGTTAAAGTGCAAAAGACCGCGCAAAGTGCCCAGATTACCTATTCATCACGACCTTACGTAGTGGAAGGTGAAACCGTTAAAACTAACTCCAACATTACCGAAACCGAGGCTAAATTCTTGTACAACAGTGAGTCAGAAGAAGCGTTAACGATTCGCGCTGGCATTAACGGTTTAATTAACTGA", "aa": "MGPYYVESFNQSQIVFKRNSEYDTHITPNLPKTREDNEKPIPTMINYFQPGATPEVFYSNYIAGGLSSAEVPYSQQEDARSRFAGTGDLRWVKVQKTAQSAQITYSSRPYVVEGETVKTNSNITETEAKFLYNSESEEALTIRAGINGLIN", "aa_hexdigest": "74d3d1287f3e7bbc992a725ac524df3e", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16991.5561, "isoelectric_point": 5.064320182800292 }, "id": "APKECPOBEN_1001", "locus": "GCA002090215_00987" }, { "type": "cds", "sequence": "contig_1", "start": 557384, "stop": 557761, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGTTTGGATGTGAAAAAGACCGTGGGGATGACCAAGAAGAATGGTGCCCAAGTTCAAATGAAGGTTGCTTCATCTGATGAAAAAGATGGTGAAGAAAAAACTGTTAAGATAACGGATGATGCTTACAATCCTGAGGATTATCAAAGCACTGATGACAGCAAATTCAACGTCTACCTCACCTCGCCCTTCCCCTTCTTGTTGAGTATGATGTCTAAGGAATTCTTCTTTCCTATTCCTCATACCCACCCGAAGGTAAAAGCAATTAAGGTAGGTAAGGACTCACCATTGGTCTACAACGAAAAGAATGGGTCGAAAATCTTAGACCAAACCAAAACTAACTTTGATGGTATCTATGGTGGTGGGGTTAACGCATGA", "aa": "MGLDVKKTVGMTKKNGAQVQMKVASSDEKDGEEKTVKITDDAYNPEDYQSTDDSKFNVYLTSPFPFLLSMMSKEFFFPIPHTHPKVKAIKVGKDSPLVYNEKNGSKILDQTKTNFDGIYGGGVNA", "aa_hexdigest": "c6abcc2ba7fb1c7be85ed988142786d6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13860.549299999986, "isoelectric_point": 6.726576423645019 }, "id": "APKECPOBEN_1002", "locus": "GCA002090215_00988" }, { "type": "cds", "sequence": "contig_1", "start": 558050, "stop": 558457, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATTTCAGCGAAAATACTGGGGTTTGTTATCAACCTTAGGGGTAAGTTCAGCAGTAGCACTCAGTGCCTGCGCTGCCCAAGCCCGCGATGTGTATGTCACCTCATCCGCATCCGACTTGTTAAAGAACAATTCGGTACCGATGAGTATGTTCAACGTGTCCCCGACCTCTTCCTTTTTTGGCAGCAAGTATGCAGGCCTAACCACTTACATTGCCACTGGTTCCAACAAAGATGATGGTGTCAATGTAGCCACTCAAACCCAAGAGAAATTGGTGTTGGAATTGGCTACCTCAGTGAAGGGGTATAAGAAGAAGGATAAAGCTACTTCAAGTCAAAAAACTTCAACTAATTCTTCTTGCACTACCACTTCTAGTGGTACTAGCACTAGTGGTGAAGATGATTGA", "aa": "MKFQRKYWGLLSTLGVSSAVALSACAAQARDVYVTSSASDLLKNNSVPMSMFNVSPTSSFFGSKYAGLTTYIATGSNKDDGVNVATQTQEKLVLELATSVKGYKKKDKATSSQKTSTNSSCTTTSSGTSTSGEDD", "aa_hexdigest": "f0917414b33a019c5cfb69fbb9bd5da1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14182.60659999998, "isoelectric_point": 9.098052406311037 }, "id": "APKECPOBEN_1003", "locus": "GCA002090215_00989" }, { "type": "tRNA", "sequence": "contig_1", "start": 558677, "stop": 558765, "strand": "+", "gene": "trnS", "product": "tRNA-Ser(cga)", "amino_acid": "Ser", "anti_codon": "cga", "score": 45.4, "nt": "TAATACTTACCCAAATGGTTTAAGGGGGCGCTTTCGAAAAGCGTTAGGTTTTTTTGCATGCGTGGGTTCGAATCCCACATTCTTGGCCA", "db_xrefs": [ "SO:0000269" ], "anti_codon_pos": [ 558711, 558713 ], "id": "APKECPOBEN_15", "locus": "GCA002090215_00990" }, { "type": "cds", "sequence": "contig_1", "start": 559032, "stop": 559349, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTGAAAAACTTTGGTGATAGTACCACTCAAGCTAAGATGCAAAGTGGAGGAAGCACTAATTCAAATGGTAATGGTTCCCAAGCTTCCCTAGCCTTCACCAAGAAAGCGCTAAGCTTACTTAAGTTCTTGGTAGATAACAAAATCTTGAAGAAGGATAAGGTGAAGGAAGCTATTATGAATCCGGATCAGTACTTAAGTAAACGTAGTAAGTTAGACAGCAACAATCAAAAGCCAACTAAAGATGACTTTATTGGTAGTGAGTTCAAAGACTTATACGAAAACGCCGCGAAGCTCAACCGCTTTAACAGCATTTGA", "aa": "MLKNFGDSTTQAKMQSGGSTNSNGNGSQASLAFTKKALSLLKFLVDNKILKKDKVKEAIMNPDQYLSKRSKLDSNNQKPTKDDFIGSEFKDLYENAAKLNRFNSI", "aa_hexdigest": "bcde0802038f352e428ce2fa0864bce5", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11668.093099999984, "isoelectric_point": 9.698124122619632 }, "id": "APKECPOBEN_1004", "locus": "GCA002090215_00991" }, { "type": "cds", "sequence": "contig_1", "start": 559392, "stop": 559616, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAACGAACAAGGTGGTGGTGATGTTATTACCTATTCTTCAGGAGCTCACATTGTGCGTACGCTGTTGCTAGCTGCGAGTCAGAGTAATGTACATTCAACGTTTACAAGCAAAGCGTTGGGTAATGATCAGACTAGTTCTATGACTAGCAGTACTACTGCAGTAACAGTTGCAAAATCTGGTGATGGACAACAGCAAACAGGTGAACAAAAGGAAGAAGACTGA", "aa": "MNEQGGGDVITYSSGAHIVRTLLLAASQSNVHSTFTSKALGNDQTSSMTSSTTAVTVAKSGDGQQQTGEQKEED", "aa_hexdigest": "d6137e3bb5fc4fd0fc631f67c5cbc948", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7648.121000000004, "isoelectric_point": 4.817468452453612 }, "id": "APKECPOBEN_1005", "locus": "GCA002090215_00992" }, { "type": "cds", "sequence": "contig_1", "start": 559774, "stop": 560139, "strand": "-", "frame": 1, "gene": null, "product": "putative protein MPN_458", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75325", "UniRef:UniRef90_P75325" ], "nt": "TTGATTCCACAACGTGAAGGTCAATACGAAAAGAACAAGAACGTTTTAACGAGTGCAACGGTTAAGGTTAACCATTATAGTTCGAAACAGGGTCAAACGCAAAGCGCATCTAAAGCGGCAGTGGCTGTTAAAGCAAACAAAGCTGCTGGTGATTCAACCACAACTTCATCGCAAACCCCCTCGGTAGAAGCGAAAAAAGTTGAAGTAACAAAGCAAAGCTTTGTTCAAGCACTTAGAAAAGTCGGCTTTACTGGCAATAACCCCCTCCACTTCAACATGAAGTTAGGGAACTCTTCGCTGTCCGCTAACGGTGTGGATTACTACAACGCCGTGAAGCAAGCACTCACCGAATTGGGTACTTGCTGA", "aa": "MIPQREGQYEKNKNVLTSATVKVNHYSSKQGQTQSASKAAVAVKANKAAGDSTTTSSQTPSVEAKKVEVTKQSFVQALRKVGFTGNNPLHFNMKLGNSSLSANGVDYYNAVKQALTELGTC", "aa_hexdigest": "e4cbc1747113297590d0422c48101e8e", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 559774, "stop": 560244, "observations": { "mutation": [ 560190, 560193 ], "stop": [ 560190, 560193 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75325", "query_cov": 1.0, "subject_cov": 0.7707006369426752, "identity": 0.992, "score": 221.0, "evalue": 2.44e-72, "gene_start": 1, "gene_end": 121, "reference_start": 36, "reference_end": 156, "reference_sequence": "MFKLNKKTLDKHPKVQIWWTITTIRFKFEKEQRLGLIPQREGQYEKNKNVLTSATVKVNHYSSKQGQTQSASKAAVAVKANKAAGDSTTTSSQTPSVEAKKVEVTKQSFVQALRKVGFTGNNPLHFNMKLGNSSLSANGVDYYNAVKQALTELGTCW" }, "paralog": false, "identity": 0.9871794871794872, "score": 299.0, "evalue": 1.66e-105, "description": "Internal stop codon at 560190, 560193. Nonsense mutation around 560190, 560193.", "psc": { "uniref90_id": "UniRef90_P75325", "product": "Uncharacterized protein MPN_458", "uniref50_id": "UniRef50_P75325", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75325", "UniRef:UniRef50_P75325" ] }, "pscc": { "uniref50_id": "UniRef50_P75325", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75325" ], "product": "Uncharacterized protein MPN_458" } }, "id": "APKECPOBEN_1006", "locus": "GCA002090215_00993" }, { "type": "cds", "sequence": "contig_1", "start": 560333, "stop": 560659, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTAACTACTTCCAACCAGGGGCAACTCCGGAAGTGTTTTATTCCAACTATATCGCTGGAGGATTGTCTTCTGCGGGGGTATCGTACTCCCAACAAGAGGATGCACGTTCACGGTTTGCGAGTACGGGGGACTTGCGCTGGGTTAAAGTGCAAAAGACCGCGCAAAGTGCCCAGATTACTTATTCATCCCGACCTTACATATTGGAAGGGGAAACCGTTAAAACTAACTCCAACATTACCGAAACCGAGGCTAAATTCTTGTACAACAGTGAGTCAGAAGAAGCGTTAACGATTCGTGCTGGCATTAACGGTTTAATTAACTGA", "aa": "MINYFQPGATPEVFYSNYIAGGLSSAGVSYSQQEDARSRFASTGDLRWVKVQKTAQSAQITYSSRPYILEGETVKTNSNITETEAKFLYNSESEEALTIRAGINGLIN", "aa_hexdigest": "fd6115de7adaea13b0ebf2e876102c04", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11899.977099999991, "isoelectric_point": 5.05215663909912 }, "id": "APKECPOBEN_1007", "locus": "GCA002090215_00994" }, { "type": "cds", "sequence": "contig_1", "start": 560804, "stop": 561190, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGTTTGGATGTGGAAAAAACCGTGGGGATGACCAACGGTAAAAATGGCACTCAGGTCAAAAAAATGACAACTGATTCCAGTAGTACTCAACAGAGTGAAGAAAAGAAGATTGAAATCACCGATGACAATTACGACCCAGAAGACTACAGGAGTACCAGTGACGATAAATTCAACGTCTATCTCACCTCACCCTTCCCCTTCTTGTTGAGTATGATGTCGAAGGAATTCTTCTTTCCTATTCCGCATACCCACCCGAAGGTAAAAGCAATTAAGGTAGGTCCAAACTCACCATTGGTTTACAACGAAAAGAACAGTGCCAAAATATTAGACCAAACCAAAACTAATTTTGATGGGATTTATGGTGGTGGTGGTGTCAACGCATGA", "aa": "MGLDVEKTVGMTNGKNGTQVKKMTTDSSSTQQSEEKKIEITDDNYDPEDYRSTSDDKFNVYLTSPFPFLLSMMSKEFFFPIPHTHPKVKAIKVGPNSPLVYNEKNSAKILDQTKTNFDGIYGGGGVNA", "aa_hexdigest": "3b5a41cfda948f3814e99337d2627c09", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14221.771199999981, "isoelectric_point": 5.6451009750366214 }, "id": "APKECPOBEN_1008", "locus": "GCA002090215_00995" }, { "type": "cds", "sequence": "contig_1", "start": 561470, "stop": 561874, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATTTCAGCGAAAATACTGGGGTTTGTTATCAACCTTAGGGGTAAGTTCAGCAGTAGCACTCAGTGCCTGCGCTGCCCAAGCACGAGATGTTTATGTTACTTCATCCGCATCCGATTTGTTAAAGAACAATTTGGTACCGATGAGTATGTTCAACGTGTCCCCGACCTCTTCCTTTTTTGGCAGTAAGTATGCAGGCCTAACCACTTACATTGCCACTGGTTCCAACAAGGACGATGGCGTTAATGTGACCAGTCAAACCCAAGAGAAATTGGTGTTGGAATTGGCTACCTCAGTTAAGGGGTATAAGAAGAAAACAAGTACCGCATCTAACATGAAGACTGCTTCTGTTTCTTCGAGCTCTTCTTCTACTGGAAACAATGGAAGTACTGAGGATGAATGA", "aa": "MKFQRKYWGLLSTLGVSSAVALSACAAQARDVYVTSSASDLLKNNLVPMSMFNVSPTSSFFGSKYAGLTTYIATGSNKDDGVNVTSQTQEKLVLELATSVKGYKKKTSTASNMKTASVSSSSSSTGNNGSTEDE", "aa_hexdigest": "a2b47465280afc3d9641232ec68a199e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14077.556799999978, "isoelectric_point": 9.239753913879394 }, "id": "APKECPOBEN_1009", "locus": "GCA002090215_00996" }, { "type": "tRNA", "sequence": "contig_1", "start": 562100, "stop": 562184, "strand": "-", "gene": "trnL", "product": "tRNA-Leu(tag)", "amino_acid": "Leu", "anti_codon": "tag", "score": 65.3, "nt": "GCACTCGTGGCGGAATGGTAGACGCGCTAGACTTAGGATCTAGTTTCATTGTGGAGTGGGGGTTCAAGTCCCTTCGAGTGCACCA", "db_xrefs": [ "SO:0000264" ], "anti_codon_pos": [ 562164, 562166 ], "id": "APKECPOBEN_26", "locus": "GCA002090215_00997" }, { "type": "tRNA", "sequence": "contig_1", "start": 562189, "stop": 562264, "strand": "-", "gene": "trnK", "product": "tRNA-Lys(ctt)", "amino_acid": "Lys", "anti_codon": "ctt", "score": 85.2, "nt": "GCATCTTTAGCTCAGTTGGTAGAGCAAATGACTCTTAATCATTGGGTCGGGGGTTCGAGCCCCTCAAGATGCACCA", "db_xrefs": [ "SO:0000265" ], "anti_codon_pos": [ 562258, 562260 ], "id": "APKECPOBEN_25", "locus": "GCA002090215_00998" }, { "type": "tRNA", "sequence": "contig_1", "start": 562277, "stop": 562352, "strand": "-", "gene": "trnT", "product": "tRNA-Thr(tgt)", "amino_acid": "Thr", "anti_codon": "tgt", "score": 89.8, "nt": "GCCGACTTAGCTCAGTTGGTAGAGCAATTGACTTGTAATCAGTAGGTCGTAGGTTCGAATCCTATAGTCGGCACCA", "db_xrefs": [ "SO:0000270" ], "anti_codon_pos": [ 562317, 562319 ], "id": "APKECPOBEN_24", "locus": "GCA002090215_00999" }, { "type": "tRNA", "sequence": "contig_1", "start": 562354, "stop": 562429, "strand": "-", "gene": "trnV", "product": "tRNA-Val(tac)", "amino_acid": "Val", "anti_codon": "tac", "score": 81.7, "nt": "GGATTGTTAGCTCAGCTGGGAGAGCATATGCCTTACAAGCATAGGGTCGGGGGTTCGATCCCCTCACAATCCACCA", "db_xrefs": [ "SO:0000273" ], "anti_codon_pos": [ 562394, 562396 ], "id": "APKECPOBEN_23", "locus": "GCA002090215_01000" }, { "type": "tRNA", "sequence": "contig_1", "start": 562437, "stop": 562511, "strand": "-", "gene": "trnT", "product": "tRNA-Thr(cgt)", "amino_acid": "Thr", "anti_codon": "cgt", "score": 81.5, "nt": "GCCGAATTAGCTCAAGGGTAGAGCGATGCACTCGTAATGCATAGGCTACAGGTTCAATTCCTGTATTCGGCACCA", "db_xrefs": [ "SO:0000270" ], "anti_codon_pos": [ 562477, 562479 ], "id": "APKECPOBEN_22", "locus": "GCA002090215_01001" }, { "type": "tRNA", "sequence": "contig_1", "start": 562513, "stop": 562589, "strand": "-", "gene": "trnE", "product": "tRNA-Glu(ttc)", "amino_acid": "Glu", "anti_codon": "ttc", "score": 58.9, "nt": "GGCGCGTTGGTGAAGAGACTTAACACACACGCCTTTCACGCGTGCATTCACGGGTTTGAATCCCGTACGCGTCACCA", "db_xrefs": [ "SO:0000259" ], "anti_codon_pos": [ 562553, 562555 ], "id": "APKECPOBEN_21", "locus": "GCA002090215_01002" }, { "type": "tRNA", "sequence": "contig_1", "start": 562598, "stop": 562672, "strand": "-", "gene": "trnN", "product": "tRNA-Asn(gtt)", "amino_acid": "Asn", "anti_codon": "gtt", "score": 84.0, "nt": "GGCCACATAGCTCAGCGGTAGAGCAACCGGCTGTTAACCGGTTGGTCACAGGTTCGATCCCTGTTGTGGCCGCCA", "db_xrefs": [ "SO:0000257" ], "anti_codon_pos": [ 562638, 562640 ], "id": "APKECPOBEN_20", "locus": "GCA002090215_01003" }, { "type": "cds", "sequence": "contig_1", "start": 562700, "stop": 563179, "strand": "-", "frame": 3, "gene": "trkG", "product": "potassium transporter TrkG", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0027D97117", "UniRef:UniRef90_UPI0027D97117" ], "nt": "ATGGTGATTGCTATGTTTATCGGGGGCAGTCCGTCTTCTACCGCCGGAGGGATTCGCACTACTACTTTAGCGGTCATCTTTTTGACGTTAAAAGCGAAGTTCCGCGGTCAAAAAGAGGTCAAGGTCTTTAAGCGTAGTATTGACGGGCAAACGGTGATTAATGCCTTCTTGGTAGCCGTCTTTGGCTTAGTGTCCGTGGTTCTTATTGCCATTCTGTTGCCGTTAAGTATGCAACAACCGCTTAGCTTTGTCGACAGCCTGTTTGAAACCACTTCTGCTTTTGGAACTGTCGGACTGTCTAGTGGGGCCACGAAGATCATGGCACTAGAGCCAACACGGAACCTGTTTAACTACTTAACGTTAGGTTTACTAATGATTATGGGGCAGGTCGGGGTGAGCAGTTCGGTACTCACTTTTGTTAAAAAACACCCTCAAGGAAACAGCTTTTCTTATCCGCGGGAAGATGTTAAAGTGGGCTAG", "aa": "MVIAMFIGGSPSSTAGGIRTTTLAVIFLTLKAKFRGQKEVKVFKRSIDGQTVINAFLVAVFGLVSVVLIAILLPLSMQQPLSFVDSLFETTSAFGTVGLSSGATKIMALEPTRNLFNYLTLGLLMIMGQVGVSSSVLTFVKKHPQGNSFSYPREDVKVG", "aa_hexdigest": "8d738b66a126f157973e098d96f98f66", "start_type": "ATG", "rbs_motif": null, "genes": [ "trkG" ], "truncated": "3-prime", "pseudogene": { "start": 562700, "stop": 563338, "observations": { "mutation": [ 563212 ], "stop": [ 563212 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0027D97117", "query_cov": 1.0, "subject_cov": 0.7464788732394366, "identity": 1.0, "score": 295.0, "evalue": 3.01e-100, "gene_start": 1, "gene_end": 159, "reference_start": 55, "reference_end": 213, "reference_sequence": "VLKNKSNQELQSLIFGPIPAASRVMQLWFGVISSRSAGFSVFPWSAESDIIKGIMVIAMFIGGSPSSTAGGIRTTTLAVIFLTLKAKFRGQKEVKVFKRSIDGQTVINAFLVAVFGLVSVVLIAILLPLSMQQPLSFVDSLFETTSAFGTVGLSSGATKIMALEPTRNLFNYLTLGLLMIMGQVGVSSSVLTFVKKHPQGNSFSYPREDVKVG" }, "paralog": false, "identity": 0.9952830188679245, "score": 400.0, "evalue": 7.11e-144, "description": "Internal stop codon at 563212. Nonsense mutation around 563212.", "psc": { "uniref90_id": "UniRef90_UPI0027D97117", "product": "potassium transporter TrkG", "uniref50_id": "UniRef50_UPI0027D97117", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0027D97117", "UniRef:UniRef50_UPI0027D97117" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0027D97117", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0027D97117" ], "product": "potassium transporter TrkG" } }, "id": "APKECPOBEN_1010", "locus": "GCA002090215_01004" }, { "type": "cds", "sequence": "contig_1", "start": 563210, "stop": 563959, "strand": "-", "frame": 3, "gene": "trkG", "product": "putative cation transporter MG322-like protein", "db_xrefs": [ "COG:COG0168", "COG:P", "GO:0005886", "GO:0008324", "GO:0030001", "SO:0001217", "UniRef:UniRef50_P75323", "UniRef:UniRef90_P75323" ], "nt": "ATGTTGCAATCAGAACGGGGTGGTTCCAAGTTAGGGACCACTAGTGAAATGATTGTGGTGTCGGTGTTATTCCTCTTTATGGTGGAGTTACTGTATGGCTTTCTGTACACCATTTTGTTTTATTTTATCCCCGCGTTTGAGTCTGCTAGTGTTTTTCAAAGTAGTGGTAAGGTGTCCAACCAATTGAAGGCCCTCATAGTCGATTCCACCAAAAGATTACCCGTAGTTCATAACCTTAACTTAGCCTTTCAGTATGGTTTTTTCCACTCCTTGTCAGCGGTTAATAATGCTGGCATTGACCTGTTGGGCGCTAATTCGTTTGCCCCGTATCGCACTAACTGGGGCATCGTGATCCAGTGGTTAGCGATTAGCCAAATTATCTTTGGTGGCATTGGTTATCCGGTGTTATTTGATGCTTATGAAGCGATTAAGAAACGGCGATTGTATGGTAAGTATTACAAACACCAGTTTAGTTTGTTTACGAAGTTAGCGGTACTCACTAACCTGATTGTCACGGCTTGGTGTTTTTTAATGCTGTTAATGGTGGAGTTTATTGTTATAACTAGTTTGACCAATACGATTGCCCATCTCAATGTCGAGAAGGCCTACTTAGTGGAGGGACTGAAAAATAAATCCAATCAAGAACTCCAAAGCTTAATCTTTGGTCCCATACCAGCGGCTAGTCGGGTGATGCAACTGTGGTTTGGGGTGATTTCGTCCCGGTCCGCTGGCTTTAGTGTGTTTCCCTGA", "aa": "MLQSERGGSKLGTTSEMIVVSVLFLFMVELLYGFLYTILFYFIPAFESASVFQSSGKVSNQLKALIVDSTKRLPVVHNLNLAFQYGFFHSLSAVNNAGIDLLGANSFAPYRTNWGIVIQWLAISQIIFGGIGYPVLFDAYEAIKKRRLYGKYYKHQFSLFTKLAVLTNLIVTAWCFLMLLMVEFIVITSLTNTIAHLNVEKAYLVEGLKNKSNQELQSLIFGPIPAASRVMQLWFGVISSRSAGFSVFP", "aa_hexdigest": "2e8f4cfaf0c0bd006df29af5d3a18073", "start_type": "ATG", "rbs_motif": null, "genes": [ "trkG" ], "truncated": "both", "pseudogene": { "start": 562907, "stop": 564259, "observations": { "mutation": [ 563212, 564013 ], "stop": [ 563212, 564013 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75323", "query_cov": 1.0, "subject_cov": 0.43915343915343913, "identity": 1.0, "score": 477.0, "evalue": 4.5e-165, "gene_start": 1, "gene_end": 249, "reference_start": 147, "reference_end": 395, "reference_sequence": "MTAAHKQKAKLLAWLKLILWGDSISQRIFHFYIYCILLGAVLLFLPFALKTDYQKVISYEVDLQGHTISKQTASYGFLDALFLAVSAFSDTGLSTTVVSETYSVFGQTVLAILLQLGGIGFVVIAFLVWRLFKLHKKGKYSFYEKLMLQSERGGSKLGTTSEMIVVSVLFLFMVELLYGFLYTILFYFIPAFESASVFQSSGKVSNQLKALIVDSTKRLPVVHNLNLAFQYGFFHSLSAVNNAGIDLLGANSFAPYRTNWGIVIQWLAISQIIFGGIGYPVLFDAYEAIKKRRLYGKYYKHQFSLFTKLAVLTNLIVTAWCFLMLLMVEFIVITSLTNTIAHLNVEKAYLVEGLKNKSNQELQSLIFGPIPAASRVMQLWFGVISSRSAGFSVFPWSAESDIIKGIMVIAMFIGGSPSSTAGGIRTTTLAVIFLTLKAKFRGQKEVKVFKRSIDGQTVINAFLVXXXVFGLVSVVLIAILLPLSMQQPLSFVDSLFETTSAFGTVGLSSGATKIMALEPTRNLFNYLTLGLLMIMGQVGVSSSVLTFVKKHPQGNSFSYPREDVKVG" }, "paralog": false, "identity": 0.9889380530973452, "score": 851.0, "evalue": 8.45e-315, "description": "Internal stop codon at 563212, 564013. Nonsense mutation around 563212, 564013.", "psc": { "uniref90_id": "UniRef90_P75323", "gene": "TrkG", "product": "Uncharacterized cation transporter MG322 homolog", "uniref50_id": "UniRef50_P75323", "cog_id": "COG0168", "cog_category": "P", "go_ids": [ "GO:0005886", "GO:0008324", "GO:0030001" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75323", "UniRef:UniRef50_P75323", "COG:COG0168", "COG:P", "GO:0005886", "GO:0008324", "GO:0030001" ] }, "pscc": { "uniref50_id": "UniRef50_P75323", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75323" ], "product": "Uncharacterized cation transporter MG322 homolog" } }, "id": "APKECPOBEN_1011", "locus": "GCA002090215_01005" }, { "type": "cds", "sequence": "contig_1", "start": 564011, "stop": 564397, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAGCTGCTCATAAACAGAAAGCTAAACTACTCGCTTGGTTAAAACTAATCCTGTGGGGTGACAGTATTTCCCAGCGCATTTTCCACTTTTACATCTATTGCATCCTGTTGGGGGCTGTACTGCTTTTCCTCCCGTTTGCGCTCAAAACCGACTACCAAAAGGTTATTAGCTATGAGGTGGATTTACAGGGTCATACCATCAGTAAACAAACGGCTTCCTATGGCTTTTTGGATGCTTTGTTTTTAGCGGTCAGTGCCTTTAGTGATACGGGGCTCTCTACTACGGTAGTGAGTGAAACCTACAGTGTGTTTGGGCAAACGGTGTTGGCCATTTTACTGCAGTTAGGGGGCATTGGTTTTGTGGTGATTGCTTTCCTTGTTTGA", "aa": "MTAAHKQKAKLLAWLKLILWGDSISQRIFHFYIYCILLGAVLLFLPFALKTDYQKVISYEVDLQGHTISKQTASYGFLDALFLAVSAFSDTGLSTTVVSETYSVFGQTVLAILLQLGGIGFVVIAFLV", "aa_hexdigest": "ebe8dac325ed4fa585280446d063174f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14113.476699999981, "isoelectric_point": 7.861161994934083 }, "id": "APKECPOBEN_1012", "locus": "GCA002090215_01006" }, { "type": "cds", "sequence": "contig_1", "start": 564432, "stop": 565127, "strand": "+", "frame": 3, "gene": "trkA", "product": "putative MG323-like protein", "db_xrefs": [ "COG:COG0569", "COG:PT", "GO:0006813", "GO:0008324", "RefSeq:WP_010874817.1", "SO:0001217", "UniParc:UPI000013961A", "UniRef:UniRef100_P75322", "UniRef:UniRef50_P75322", "UniRef:UniRef90_P75322" ], "nt": "ATGAAACGTGCCGATTTTTGCATTATTGGCCTTGGTAGGTTTGGGATGCAAGTAGCACATTCTTTAAAAGAGAACAACTTTACCCTGGTTTTGATCGATAATGATCAACACAAAACCAATACTGCTGCCCAGGAATTTGACCATGTAGTGTGTTGTGATGGGAGTAACTTAACGGCGTTAGCTGAGCTCCAACTAGAGGAGTTTTCGGCAGTCATAGTTGGGGTAACTAACATAGAAGCGAGCATTATGATTTGTGCTAACCTTCGGGAATTGGGTCAAAAAAACATTATTGCCCGTGCTAAAAACGAAGTGCATAACCGGGTGTTACGTACTATGGGTATTAGGGAAGCTTTGATCCCGGAAAAGATTGTCGGCAAGAACCTTGTCATTCGTTTAATTCACGGCATGGAAACCGAAATTATTAACCTTGGCAATGACATCCTCTTTGTTCGCGCTCCGGTGAACAACAAGAAGCTCTTTAATCGTACGTTGGGGGAAATTAACATCCGTGAACACACGAATGCTAACCTCATCTCAATTATGCGGAATGGCAAAGATGTCGTCTTTCCTTTGGGTCCGAATACCCAGATTCAACCAGGGGATGTAATTACAGCCGTGTGTCAATTAAGTGGCGTGAACCAGTATTTGCGCTACATTAATCCTAACGACAAGAATAAGTACAAAGCGAGTGAATAA", "aa": "MKRADFCIIGLGRFGMQVAHSLKENNFTLVLIDNDQHKTNTAAQEFDHVVCCDGSNLTALAELQLEEFSAVIVGVTNIEASIMICANLRELGQKNIIARAKNEVHNRVLRTMGIREALIPEKIVGKNLVIRLIHGMETEIINLGNDILFVRAPVNNKKLFNRTLGEINIREHTNANLISIMRNGKDVVFPLGPNTQIQPGDVITAVCQLSGVNQYLRYINPNDKNKYKASE", "aa_hexdigest": "ed910f8d08c6738e9a02d4fb3d1effa7", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013961A", "ncbi_nrp_id": "WP_010874817.1", "uniref100_id": "UniRef100_P75322", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013961A", "RefSeq:WP_010874817.1", "UniRef:UniRef100_P75322" ] }, "ips": { "uniref100_id": "UniRef100_P75322", "uniref90_id": "UniRef90_P75322" }, "psc": { "uniref90_id": "UniRef90_P75322", "gene": "TrkA", "product": "Uncharacterized protein MG323 homolog", "uniref50_id": "UniRef50_P75322", "cog_id": "COG0569", "cog_category": "PT", "go_ids": [ "GO:0008324", "GO:0006813" ] }, "pscc": { "uniref50_id": "UniRef50_P75322", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75322" ], "product": "Uncharacterized protein MG323 homolog" }, "genes": [ "trkA" ], "id": "APKECPOBEN_1013", "locus": "GCA002090215_01007" }, { "type": "cds", "sequence": "contig_1", "start": 565189, "stop": 565623, "strand": "+", "frame": 1, "gene": null, "product": "putative protein MPN_462", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75321", "UniRef:UniRef50_UPI001375E5B8", "UniRef:UniRef90_P75321" ], "nt": "GTGGTGTACTATGAGCAGTTGCAAAGGGGATTAGCGCTCCCTAACCAAGGGAGTTCGTCAGGCTCAGACAGCACTAACCAAACAGGCGCAATGTTTGGCTTGAAGGTGAAGGATGCAACCGTGGATAGTTCGAAGCAATCAACGGAAAGCTTAAAGGGCGAAGAATCGAGTTCCAGTTCCACCACATCTTCCACCTCCACCACCCAACGTGGGGGTTCGTCAGGGGACACCAAAGTCAAGGCGTTGCAGGTGGCGGTGAAAAAGAAATCGGACTCGGAGGACAATGGTCAGATCGAACTTGAAACCAACAACCTCGCCAACGCCCCGATTAAACGGGGCTCCAATAACAACCAGCAAGTCCAACTCAAGGCGGACGATTTTGGTACTTCCCCTTCCAGTTCGGAATCTGGTCAATCAGGCACCCCCACCCCCTGA", "aa": "MVYYEQLQRGLALPNQGSSSGSDSTNQTGAMFGLKVKDATVDSSKQSTESLKGEESSSSSTTSSTSTTQRGGSSGDTKVKALQVAVKKKSDSEDNGQIELETNNLANAPIKRGSNNNQQVQLKADDFGTSPSSSESGQSGTPTP", "aa_hexdigest": "c75d4d955b19f4b6d10440fed5dbabac", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001375E5B8", "query_cov": 0.9097222222222222, "subject_cov": 0.8618421052631579, "identity": 0.6559999999999999, "score": 142.0, "evalue": 1.13e-40, "valid": false, "uniref50_id": "UniRef50_UPI001375E5B8" }, "pscc": { "uniref50_id": "UniRef50_UPI001375E5B8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001375E5B8" ] }, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 565189, "stop": 565926, "observations": { "mutation": [ 565621, 565669, 565783, 565798, 565822 ], "stop": [ 565621, 565669, 565783, 565798, 565822 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75321", "query_cov": 1.0, "subject_cov": 0.48, "identity": 0.986, "score": 224.0, "evalue": 3.68e-71, "gene_start": 1, "gene_end": 144, "reference_start": 1, "reference_end": 144, "reference_sequence": "MVYYEQLQRGLALPNQGSSSGSDSTNQTGAMFGLKVKDATVDSSNQSTESLKGEESSSSSTTSSTSTTQRGGSSGDTKVKALQVAVKKKSDSEDNGQIELETNNLANAPIKRGSNNNQQVQLKADDFGTAPSSSESGQSGTPTPWTPWLATEQIHKDLPKWSASILILYDAPYARNRTAIDRVDHLDPKVMTANYPPSWRTPKWNHHGLWDWKARDVLLQTTGFFNPRRHPEWFDQGQAVADNTQTGFDTDDTDNKKQGFRKQGEQSPAPIALPFEAYFANIGNLTWFGQALLVFGICLS" }, "paralog": false, "identity": 0.963265306122449, "score": 432.0, "evalue": 3.08e-155, "description": "Internal stop codon at 565621, 565669, 565783, 565798, 565822. Nonsense mutation around 565621, 565669, 565783, 565798, 565822.", "psc": { "uniref90_id": "UniRef90_P75321", "product": "Uncharacterized protein MPN_462", "uniref50_id": "UniRef50_P75321", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75321", "UniRef:UniRef50_P75321" ] }, "pscc": { "uniref50_id": "UniRef50_P75321", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75321" ], "product": "Uncharacterized protein MPN_462" } }, "id": "APKECPOBEN_1014", "locus": "GCA002090215_01008" }, { "type": "cds", "sequence": "contig_1", "start": 565603, "stop": 566058, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCCGAACCAGGTGAGGTTGCCAATGTTGGCGAAGTACGCTTCAAACGGGAGGGCGATGGGGGCAGGGGATTGTTCGCCTTGTTTGCGAAAGCCCTGCTTTTTATTATCGGTGTCATCTGTATCAAACCCGGTTTGGGTATTCTCCGCGACCGCTTGGCCCTGGTCAAACCACTCGGGGTGGCGGCGCGGGTTGAAGAACCCGGTGGTTTGGAGCAGGACATCGCGCGCCTTTCAGTCCCACAACCCGTGGTGGTTTCACTTGGGCGTTCTTCAACTGGGCGGATAGTTCGCGGTCATCACCTTGGGATCCAAGTGATCAACGCGATCAATGGCGGTACGATTGCGCGCATAAGGCGCATCGTACAGAATCAGGATCGAGGCGGATCATTTGGGGAGGTCCTTGTGAATTTGCTCGGTCGCAAGCCACGGCGTTCAGGGGGTGGGGGTGCCTGA", "aa": "MPEPGEVANVGEVRFKREGDGGRGLFALFAKALLFIIGVICIKPGLGILRDRLALVKPLGVAARVEEPGGLEQDIARLSVPQPVVVSLGRSSTGRIVRGHHLGIQVINAINGGTIARIRRIVQNQDRGGSFGEVLVNLLGRKPRRSGGGGA", "aa_hexdigest": "5e970773660fdd44a75b92cc369ecf7c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15897.39109999998, "isoelectric_point": 11.366952705383298 }, "id": "APKECPOBEN_1015", "locus": "GCA002090215_01009" }, { "type": "cds", "sequence": "contig_1", "start": 566234, "stop": 566809, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGGACAGAACAAGTTGGTGGCGCTGGTCCGCTCAGCGTTGGTCAGGGTGAAGTTTCAAGATAGTTCGAGCCAACAAGGCACTGAGAGTAATGGCCAAGACCAAAATGCCCTTAGTTTTGATACCACCAAGTCCCAACAAGCCTTACAGCAAGCTGGCGATCAATCAGGAACTTCATCTAACTCCACACAAGAAGACTTCGCCAGCTACATCCTCATCTTTAAAGCCGCGCCCAGGGCCACGTGGGTATTTGAACGCAAGATTAAGTTGGCGTTGCCCTACGTTAAGCAGGAAAGTCAGGGTTCCGACGATCAAGGTTCCAATGGTAAGGGCTCCCTCTACAAAACCCTCCAAGACCTCCTCGTCGAACAACCCGTGACCCCTTACACCCCGAATGCGGGGTTAGTCCGGGTGAATGGGGTTGCTCAGGATACGGTTCATTTTGGTTCGGGTCAAGAATCGAGTTGGAATTCCCAACGTTCCCAAAAAGGCCTTAAAAACAACCCCGGACCCAAAGCCGTCACCGGCTTTAAGCTCGATAAGGGCCGCGCGTACCAGAAGTTAAATGAAGCTTGA", "aa": "MGQNKLVALVRSALVRVKFQDSSSQQGTESNGQDQNALSFDTTKSQQALQQAGDQSGTSSNSTQEDFASYILIFKAAPRATWVFERKIKLALPYVKQESQGSDDQGSNGKGSLYKTLQDLLVEQPVTPYTPNAGLVRVNGVAQDTVHFGSGQESSWNSQRSQKGLKNNPGPKAVTGFKLDKGRAYQKLNEA", "aa_hexdigest": "e5593da432d099488cd452d06ba43b21", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 20744.690999999988, "isoelectric_point": 9.396218681335448 }, "id": "APKECPOBEN_1016", "locus": "GCA002090215_01010" }, { "type": "cds", "sequence": "contig_1", "start": 566813, "stop": 567139, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTATGAACCCCTGGATTCGACGAAGGAGGGGAAGGAGAAAAACGAATCGGAGTGGAAGTCTTCCGGGGCGAGTGAGGCAAAAAATGATGCCCCGTTGGTGGGATCAACCGGGAGTCAAATGGCTGCTGTTACAGACAATCAGCAATCAGGTGATAACAATGGATTGGTATCGCTTGCACAGCGCTCCACTACTGTTGCTGTCCAAAAAAGTGACTCTTCTGGTTCTCAAGGCCAAGGCACAACCGACAACAAATTCCAAAAATATTTAAACACCACCCAGGCCCTGCACCAGATGGGGGTGATAGTGCCATCTTTGGAAACGTGA", "aa": "MYEPLDSTKEGKEKNESEWKSSGASEAKNDAPLVGSTGSQMAAVTDNQQSGDNNGLVSLAQRSTTVAVQKSDSSGSQGQGTTDNKFQKYLNTTQALHQMGVIVPSLET", "aa_hexdigest": "c75d6f54ce32396215e55f305233594a", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11411.227499999995, "isoelectric_point": 4.809567832946779 }, "id": "APKECPOBEN_1017", "locus": "GCA002090215_01011" }, { "type": "cds", "sequence": "contig_1", "start": 567443, "stop": 568081, "strand": "+", "frame": 2, "gene": "mgpC", "product": "MgpC family cytadherence protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002246AD21", "UniRef:UniRef90_UPI002246AD21" ], "nt": "TTGGGGTTTAAAGATGACTCAAATTCGGATTCCAAAAATTCGAATTTGAAGGCCCAAGGCCTCACCCAACCCGCCTACCTCATCGCCGGTCTTGACGTTGTGGCCGACCACCTCGTCTTTGCGGCCTTCAAAGCGGGGGCAGTGGGGTATGATATGACGACTGATTCGAACGCTTCCACCAAAGACCAAGTGCTCGCCTGGTCGACCACGGCCGGATTGGACAGTGATGGGGGGTACAAGGCCTTGGTGGAAAACACGGCCGGATTGAACGGACCGATTAATGGCTTGTTTATCCTGTTAGACACCTTTGCCTATGTGACCCCGGTGAGTGGGATGAAAGGGGGGAGTAAGAATACGGAGGCAGTGCAAACCAAGTATCCCGTTAAGGATGATAGTAAGGCGAGTGCCAAAATTGCTTCCTTAATTAATGCCAGTCCACTCAACAGTTATGGGGATGATGGCGTGACCGTGTTTGATGCCCTGGGCCTTAACTTTAACTTTAAGTTGGACGAGGCGCGCTTGCCATCGCGCACCGACCAACTGCTTGTGTATGGGATTGTAAACGAAAGTGAACTGAAGTCCGCGCGCGAAAATGCCCAGTCGACATCCGATGATAATTCAAACACCAAAGTCAAGTGA", "aa": "MGFKDDSNSDSKNSNLKAQGLTQPAYLIAGLDVVADHLVFAAFKAGAVGYDMTTDSNASTKDQVLAWSTTAGLDSDGGYKALVENTAGLNGPINGLFILLDTFAYVTPVSGMKGGSKNTEAVQTKYPVKDDSKASAKIASLINASPLNSYGDDGVTVFDALGLNFNFKLDEARLPSRTDQLLVYGIVNESELKSARENAQSTSDDNSNTKVK", "aa_hexdigest": "cd50983f141002c00ab8a4ddc6b21e4d", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002246AD21", "query_cov": 0.9292452830188679, "subject_cov": 0.8565217391304348, "identity": 0.934, "score": 348.0, "evalue": 6.23e-120, "valid": true, "product": "MgpC family cytadherence protein", "uniref50_id": "UniRef50_UPI002246AD21" }, "pscc": { "uniref50_id": "UniRef50_UPI002246AD21", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002246AD21" ], "product": "MgpC family cytadherence protein" }, "genes": [ "mgpC" ], "id": "APKECPOBEN_1018", "locus": "GCA002090215_01012" }, { "type": "cds", "sequence": "contig_1", "start": 568835, "stop": 569197, "strand": "+", "frame": 2, "gene": null, "product": "UPF0134 protein MPN_100", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75254", "UniRef:UniRef90_P75592" ], "nt": "GTGAACACGGAATACGTAACGCGTAAAGAGTTCAACGAGTACAAAAACTCAAACAACCAACGACTTACAAAGATTGAAACCACTTTGGCCGCCCAAGGTGAACAAATCAACAAATTGACTCAAACTGTTGAAAAACAGGGCGAGCAAATCAATCAATTAGTTCAAGTTGTGCTTCTTCACGGCGAGCAAATTAACAAACTGACTCAAACTGTTGAAAAACAGGGCGAGCAAATTAGAGAACTTCAAGTAGAGCAAAAATTTCAGGGTGAACAAATAAAAGCTCAAGGAAAAACGCTTAAGTCGATCCTACAAGCGCTTGGAGGAATAAATAAACGCTTGGACAAAATTGATCCACCTAAATAG", "aa": "MNTEYVTRKEFNEYKNSNNQRLTKIETTLAAQGEQINKLTQTVEKQGEQINQLVQVVLLHGEQINKLTQTVEKQGEQIRELQVEQKFQGEQIKAQGKTLKSILQALGGINKRLDKIDPPK", "aa_hexdigest": "4dbddefcbd4c1ec3dd8b4b788f5295c9", "start_type": "GTG", "rbs_motif": "AACAA", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 568667, "stop": 569197, "observations": { "mutation": [ 568748 ], "stop": [ 568748 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75592", "query_cov": 1.0583333333333333, "subject_cov": 0.6939890710382514, "identity": 0.8190000000000001, "score": 76.6, "evalue": 4.32e-15, "gene_start": 1, "gene_end": 120, "reference_start": 57, "reference_end": 183, "reference_sequence": "MKEKIPFYNEKEFHDMVKKTKKGTFSGWYIIDKDNKSVEFSGSFNRQFKLNKPVIPVNTEYVTRKEFNEYKVSNDQRLTKIETTLAAQGEQINKLTQTVEKQGEQINQLVQVVLLHGEQINKLTQTVEKQGEQIKELQVEQKAQGEQIKAQGKQIKAQGKTLKSILQALGGINKRLDKIDPPK" }, "paralog": false, "identity": 0.8469945355191257, "score": 184.0, "evalue": 6.62e-60, "description": "Internal stop codon at 568748. Nonsense mutation around 568748.", "psc": { "uniref90_id": "UniRef90_P75592", "product": "UPF0134 protein MPN_100", "uniref50_id": "UniRef50_P75254", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75592", "UniRef:UniRef50_P75254" ] }, "pscc": { "uniref50_id": "UniRef50_P75254", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75254" ], "product": "UPF0134 protein MPN_524" } }, "id": "APKECPOBEN_1019", "locus": "GCA002090215_01013" }, { "type": "cds", "sequence": "contig_1", "start": 569663, "stop": 569752, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAGCGAAAGATTGAACATTTCCAGATGACATACCAAAGTTTTAAGGATTTGTCAGTTGAGGCTAAGTTAAGCTATACCTTTAATTGA", "aa": "MERKIEHFQMTYQSFKDLSVEAKLSYTFN", "aa_hexdigest": "0ab2fdb5e03d0b78047585486ea9391e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3572.028800000001, "isoelectric_point": 6.523547554016113 }, "id": "APKECPOBEN_1020", "locus": "GCA002090215_01014" }, { "type": "cds", "sequence": "contig_1", "start": 569839, "stop": 570087, "strand": "-", "frame": 1, "gene": null, "product": "putative lipoprotein MPN_467", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P75316", "UniRef:UniRef90_P75316" ], "nt": "ATGCGCAAAAAGAGATTGTTGTCAAGGATTTCCTTTAGTTCTCTATTTTTATTATGTGGAACATTGTTAAGTGCTTGCACTGGTATCCAAGCAGATCTTAGAAATCTTATTAAAGAAACCACTGGTAAAGACATTGATTTGTCTAAAGCAATTAAAACTAAAGAAGGCAAAAAGAACATAATAGCTTCTTTAAAAAAGTCTTACGAAGTTAATCCGAGAGATACAACAAAATTGTTGTTAGATGCTTGA", "aa": "MRKKRLLSRISFSSLFLLCGTLLSACTGIQADLRNLIKETTGKDIDLSKAIKTKEGKKNIIASLKKSYEVNPRDTTKLLLDA", "aa_hexdigest": "13dfe1101522dd7d52ba9752cb93c633", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75316", "query_cov": 1.0, "subject_cov": 0.8118811881188119, "identity": 1.0, "score": 153.0, "evalue": 5.5e-47, "valid": true, "product": "Uncharacterized lipoprotein MPN_467", "uniref50_id": "UniRef50_P75316", "go_ids": [ "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75316", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75316" ], "product": "Uncharacterized lipoprotein MPN_467" }, "genes": [], "id": "APKECPOBEN_1021", "locus": "GCA002090215_01015" }, { "type": "cds", "sequence": "contig_1", "start": 570483, "stop": 570680, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0H3DJX7", "UniRef:UniRef90_A0AAN4XEA6" ], "nt": "ATGAAAAAAGTTAAAGCATTAAGGAATCTTTTTCTAGAAAAAGCCAGTGAAAAACTTGAATACCCCAAAATTGCTTCAATGGTAGCTTACTTTGATCCAAAGGAAGATGGTACTTGTGAATTCATTATGAAAATTAAAACTGTCAATGAAGTTGTTGACACTGAGACGACAAATAAAGAAAAGCCTTTAGGCACTTGA", "aa": "MKKVKALRNLFLEKASEKLEYPKIASMVAYFDPKEDGTCEFIMKIKTVNEVVDTETTNKEKPLGT", "aa_hexdigest": "b806d581cdc531a29753cf0eb307822d", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0AAN4XEA6", "query_cov": 1.0, "subject_cov": 0.8552631578947368, "identity": 0.9690000000000001, "score": 123.0, "evalue": 1.45e-35, "valid": true, "uniref50_id": "UniRef50_A0A0H3DJX7" }, "pscc": { "uniref50_id": "UniRef50_A0A0H3DJX7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0H3DJX7" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7440.6306, "isoelectric_point": 7.800045967102052 }, "id": "APKECPOBEN_1022", "locus": "GCA002090215_01016" }, { "type": "cds", "sequence": "contig_1", "start": 570719, "stop": 571051, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACCACCCACAGTCGTCGTCTGACTGTCACCAAGCGTGCCCTGGACATTCGCTTTGAGACCAACGCCGACATTGCTAAAAGTGGGTGCAACATCATTGGACTGCGTGGTGGAACTTGTGTTGCCCGCCGGCGAGCCGTTGGTGGCGCTGTGACCCGCTCCAGCGACCCCCTTATATCCATTCCCATACCTGTACCCATTCCCAATCCAGGGAATATAATCCAACACCTGGTCAAGGAGTGCCCCCGCGAGGTTGAACCCGGTGGAGAAACGGGAAAGCGTGGGGGAGAAATAAAAATACCAACAGCAAGTTTTAACACTTACCGTTGGTAA", "aa": "MTTHSRRLTVTKRALDIRFETNADIAKSGCNIIGLRGGTCVARRRAVGGAVTRSSDPLISIPIPVPIPNPGNIIQHLVKECPREVEPGGETGKRGGEIKIPTASFNTYRW", "aa_hexdigest": "c1c0f67f3fb62067290dae71f8b93599", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11917.624299999989, "isoelectric_point": 10.277694892883297 }, "id": "APKECPOBEN_1023", "locus": "GCA002090215_01017" }, { "type": "cds", "sequence": "contig_1", "start": 571232, "stop": 571486, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGATCCTTTTTTTATTGCTTTATTTGACCTTAAATTAATTATTCACCAACACCGGGATGCTGCCCAACAGGCCGCGGAGGAACAGCTGGGCGCGCTGGGCGTTGTTCTTGTTGGTGAAGCTCAAAGCGTTCGGTCAGTCGGATGTGGGGGTGAGGTTGGGGGGGAGGTTGGTGTAGTTGGTCAAGTTGTCGCCCGATGTGGCTTCTTGAACACTGATCGAATTACCACTTTTATCAGCCTTATCCTGCTTTAA", "aa": "MDPFFIALFDLKLIIHQHRDAAQQAAEEQLGALGVVLVGEAQSVRSVGCGGEVGGEVGVVGQVVARCGFLNTDRITTFISLILL", "aa_hexdigest": "604b376762c79da35fb25ccc86a7feb5", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8866.166099999993, "isoelectric_point": 4.817638969421386 }, "id": "APKECPOBEN_1024", "locus": "GCA002090215_01018" }, { "type": "ncRNA", "class": [ "RNase_P_RNA", "SO:0000386" ], "sequence": "contig_1", "start": 571513, "stop": 571842, "strand": "-", "gene": "RNaseP_bact_b", "product": "Bacterial RNase P class B", "score": 265.8, "evalue": 6.9e-29, "db_xrefs": [ "RFAM:RF00011", "SO:0000386" ], "nt": "GCTGTCGGTCAGTTGCTGCTCAATGAGTAGAGGAAAGTCCATGCTTGCACTTGCTGGAACGCAAGTAGTGTTTGTGTAAATTGAACAAATAAGATTTAGGACGCTAATAGACGTCACGACTGGACTAGTCTAAGGTCTTTGACTATGATGATGTCCGTAAAGTACCACAGAGACGAGCTTAGTGAAAACTAAGTATGAAACGCGGTAAACTCCACAAGCAAGAAACCTAAATTTTGGTAAGGGAATGTTACTTTAAGAAAAATAATTAAAGTAATGACAGGATTACTGTAGATAAATAACTGACTTTAAAACAGAACATGGCTTATTGGC", "id": "APKECPOBEN_42", "locus": "GCA002090215_01019" }, { "type": "tmRNA", "sequence": "contig_1", "start": 571867, "stop": 572253, "strand": "+", "gene": "ssrA", "product": "transfer-messenger RNA, SsrA", "db_xrefs": [ "SO:0000584" ], "tag": { "start": 571950, "stop": 572030, "aa": "DKNNDEVLVDPMLIANQQASINYAFA", "nt": "GACAAAAATAACGACGAAGTTTTGGTAGATCCAATGTTGATCGCTAACCAACAAGCAAGTATCAACTACGCTTTCGCTTAG" }, "nt": "GGGGATGTAGAGGTTTTGACATAATGTTGAAAGGAAAACAGTTGCAGTGGGGTATGCCCCTTACAGCTCTAGGTATAATAACCGACAAAAATAACGACGAAGTTTTGGTAGATCCAATGTTGATCGCTAACCAACAAGCAAGTATCAACTACGCTTTCGCTTAGAACATACTAAAGCTACACGAATTGAATCGCCATAGTTTGGTTCGTGTCACAGTTTATGGCTCGGGGTTAACTGGTTCAACTTAATCCTTAAATTATGAACTTATCGTTTACTTGTTTGTCTTATGATCTAAAGTAAGCGAGACATTAAAACATAAGACTAAACTGTAGAAGCTGTTTTACCAATCCTTTATGGAAACGGGTTCGATTCCCGTCATCTCCACCA", "id": "APKECPOBEN_38", "locus": "GCA002090215_01020" }, { "type": "cds", "sequence": "contig_1", "start": 572344, "stop": 572559, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGCTCTTTTTGGTGTTATCGGACCAACACTTTTTCCCGCGTACGACAACGCAACTAAAAAGTAATCCTAACCTGTCACTGTTCTTTCGGATTTCTCACAAAGACAACGTTTTTAGTTCGCGCTTAAACCAACTAGGAGCGTTTGCCACCGCTTTATGTATTACGCTTGTGGTGTATGAATTGCCTTTTTTAGGACTGTTTGCCTTTAACTGA", "aa": "MLLFLVLSDQHFFPRTTTQLKSNPNLSLFFRISHKDNVFSSRLNQLGAFATALCITLVVYELPFLGLFAFN", "aa_hexdigest": "5ce299020c964f1922fcedb3f2bebbe7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8125.423799999997, "isoelectric_point": 9.18495578765869 }, "id": "APKECPOBEN_1025", "locus": "GCA002090215_01021" }, { "type": "cds", "sequence": "contig_1", "start": 572791, "stop": 573027, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTAAGATCAACGATAAACTCACCGAAATTAATACCGTTGAATACGAAGTTGCTTCCAAACACAGTCAGTACCTCTTCTACAGCCGTTTTGGTCTGTTAGATACCGCTGCTTACTTTTTGTTCTTGCTGTCTTTTTTTGTGACAGCAGTGATGTTCTTGGTGGGTATCTTTCACACCGAGCAGTTTACCTTAAACGATCAAAACCAGGGCATTAGTGGTTTTTACTTGTTTTGA", "aa": "MSKINDKLTEINTVEYEVASKHSQYLFYSRFGLLDTAAYFLFLLSFFVTAVMFLVGIFHTEQFTLNDQNQGISGFYLF", "aa_hexdigest": "2d8ba9e1b0fd9fff7c4b0f778e406837", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9082.277099999992, "isoelectric_point": 5.006230735778809 }, "id": "APKECPOBEN_1026", "locus": "GCA002090215_01022" }, { "type": "cds", "sequence": "contig_1", "start": 573068, "stop": 574132, "strand": "-", "frame": 3, "gene": "pepP", "product": "Putative Xaa-Pro aminopeptidase", "db_xrefs": [ "COG:COG0006", "COG:E", "EC:3.4.11.9", "GO:0004177", "GO:0006508", "GO:0008237", "GO:0046872", "RefSeq:WP_010874826.1", "SO:0001217", "UniParc:UPI0000125A20", "UniRef:UniRef100_P75313", "UniRef:UniRef50_P47566", "UniRef:UniRef90_P75313" ], "nt": "ATGCATAACGAACTGCAACAAAAGCTAGCAGTTTTACACAAACTGCTCCAAGACAATAAAGCTGATGCCATCTTAATTGGCTCAGACCAAAACCGCTTTTGGCTTACTGGTTTTCCCTCCAGTGCTGGTTGGTTAGTGGTTCACAAGCAAAGGGTTAACCTCTTTATTGACGGGCGTTACTTTGAAGCCGCTAAAACGGCAATTGATCCCCTAGTCAAAGTGGAATTATTTACCACTTATAAACAAGTAAAGGCATTATGTGAACAAGTGGGTGTGAAACACCTCTTGATTGAAGGGGATTACCTCACTTTTAACTACCAAAATTTCATTAAGGAGTTGTGTGCGCAGTACACGGTCATTAACGCCCAGGAAATTCGCCGACAAAAGCTCCCGAGTGAAATCTTAGCGATTGAAAAGGTAGTGGAAATTACCCGCAAAGTAGCGGTAAAACTCAAGCGCTTTATCCAACCAGGCATGACGGAGTTGTTCATTGCGCAGTGGATTACTGACCAATTGGTCAAAGCTGGTGGGGCAAAAAACTCGTTTGACCCTATTGTAGCTACTGGTAAGAACGGTGCTAATCCACACCACAAGCCTTCCAAGTTAAAGGTAAAATCAGGTGATTTTGTAACCTGTGACTTTGGCACCATTTACAACGGTTACTGTTCCGATATTACCCGCACCTTCTTAGTTGGTAAAAAACCTAATAATGAAGTGTTGCTCAAGGCCTATAAAAAGGTTGATGAAGCCAATATGGCCGGCATTAACGCTGCTAATACCCAACTCACCGGTGCGGAAGTTGACAAGGTGTGCCGTGACATTATTGAAGCATCGGAGTTCAAAGATTACTTTGTTCACAGCACTGGTCATGGTGTTGGACTTGACATTCACGAAATGCCAAACGTATCAACGTCCTATAACAAGTTGTTGTGTGAAAATGCTGTCATTACGATTGAACCGGGTATTTATATCCCTAGTGTTGGTGGCATCCGCATTGAGGACATGGTGTTAGTTAAAGACCACAAGAGTGTGTGGTTATCAGCCAAGATTCCGCGTGCCTTCTAA", "aa": "MHNELQQKLAVLHKLLQDNKADAILIGSDQNRFWLTGFPSSAGWLVVHKQRVNLFIDGRYFEAAKTAIDPLVKVELFTTYKQVKALCEQVGVKHLLIEGDYLTFNYQNFIKELCAQYTVINAQEIRRQKLPSEILAIEKVVEITRKVAVKLKRFIQPGMTELFIAQWITDQLVKAGGAKNSFDPIVATGKNGANPHHKPSKLKVKSGDFVTCDFGTIYNGYCSDITRTFLVGKKPNNEVLLKAYKKVDEANMAGINAANTQLTGAEVDKVCRDIIEASEFKDYFVHSTGHGVGLDIHEMPNVSTSYNKLLCENAVITIEPGIYIPSVGGIRIEDMVLVKDHKSVWLSAKIPRAF", "aa_hexdigest": "1bdb905ea2ec643b5ea71f618a04a30a", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000125A20", "ncbi_nrp_id": "WP_010874826.1", "uniref100_id": "UniRef100_P75313", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000125A20", "RefSeq:WP_010874826.1", "UniRef:UniRef100_P75313" ] }, "ips": { "uniref100_id": "UniRef100_P75313", "uniref90_id": "UniRef90_P75313" }, "psc": { "uniref90_id": "UniRef90_P75313", "gene": "pepP", "product": "Putative Xaa-Pro aminopeptidase", "ec_ids": [ "3.4.11.9" ], "uniref50_id": "UniRef50_P47566", "cog_id": "COG0006", "cog_category": "E", "go_ids": [ "GO:0004177", "GO:0046872", "GO:0008237", "GO:0006508" ] }, "pscc": { "uniref50_id": "UniRef50_P47566", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47566" ], "product": "Putative Xaa-Pro aminopeptidase" }, "genes": [ "pepP" ], "id": "APKECPOBEN_1027", "locus": "GCA002090215_01023" }, { "type": "cds", "sequence": "contig_1", "start": 574312, "stop": 575193, "strand": "-", "frame": 1, "gene": "degV", "product": "DegV domain-containing MG326-like protein", "db_xrefs": [ "COG:COG1307", "COG:I", "GO:0008289", "RefSeq:WP_010874828.1", "SO:0001217", "UniParc:UPI0000139625", "UniRef:UniRef100_P75312", "UniRef:UniRef50_P75312", "UniRef:UniRef90_P75312" ], "nt": "ATGAAGACCGCGATAATTACTGACTCGACTGCCTCAATTAAGGAAGGAGAGATCCAAGACGTTTACGTGTTACCGTTACAGGTCATTATTAACGGTCAAGACACTTACCGCGATGGTAAGGACATTGACTATGATCGAGTATACCAGTTGTTGAAGGAACACCCCCAAGGGTTAAACATTTCCACATCACTACCACGCCAAGCTGACTTAATTGAGTTAATTGAAGCCATTAAAGACAAGTACGATCGCTTTGTTTTTTTACCTCTCAGTAAGGGTTTGAGTGGTACGTATGATATGATTGTCCAAGCGGTTAAGCCACTCAGTACGCCCAAAAAAGAGTTTGTGGTCTTGGAAACAAGTGACATTGCCATTTCCCTGAAATGGTTAGTACAAGAGGTGAAGGCATTAACTGACACTAACTGCCCAACTAAAGCAATTGCTGAAGTGGTTGACCAGCACAAACAAAGTATTTTTACAGCTGTGACTGTAAAAAACTTAGTGCAGCTACGTAAAGGTGGGCGCATTTCTGGATTAAAGAAAATTATTGCCACACTTTTACGGGTTAAACCGATTATCTTCTTTGATAAAGGGGTAAATACTTTGAGCGGCAAGGCCTTTACCTTTGTCCAAGCATTGGAGAAAATCTTTACCTTTGTCAAATCAAAATTTGGTGATAACTTTAAGATCAAACGGATTGGTTTTTGCAATGCTTTTACCCCAGTTAAGGCCAAGGAAGTTAAAGCTTTAATTTTGGATTTTCTCCACACTAACAAAATCACTTTACAACGTGAAATCGAAAGCTCGTTTATCACAAGTGCCATTATTGCGCATACCGGAATAGATGCGTTTTCGATCAGTTTATTACTCGATAAAAATAAATAA", "aa": "MKTAIITDSTASIKEGEIQDVYVLPLQVIINGQDTYRDGKDIDYDRVYQLLKEHPQGLNISTSLPRQADLIELIEAIKDKYDRFVFLPLSKGLSGTYDMIVQAVKPLSTPKKEFVVLETSDIAISLKWLVQEVKALTDTNCPTKAIAEVVDQHKQSIFTAVTVKNLVQLRKGGRISGLKKIIATLLRVKPIIFFDKGVNTLSGKAFTFVQALEKIFTFVKSKFGDNFKIKRIGFCNAFTPVKAKEVKALILDFLHTNKITLQREIESSFITSAIIAHTGIDAFSISLLLDKNK", "aa_hexdigest": "46556300cca56294085e003b00954d5f", "start_type": "ATG", "rbs_motif": "AATAA", "ups": { "uniparc_id": "UPI0000139625", "ncbi_nrp_id": "WP_010874828.1", "uniref100_id": "UniRef100_P75312", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139625", "RefSeq:WP_010874828.1", "UniRef:UniRef100_P75312" ] }, "ips": { "uniref100_id": "UniRef100_P75312", "uniref90_id": "UniRef90_P75312" }, "psc": { "uniref90_id": "UniRef90_P75312", "gene": "DegV", "product": "DegV domain-containing protein MG326 homolog", "uniref50_id": "UniRef50_P75312", "cog_id": "COG1307", "cog_category": "I", "go_ids": [ "GO:0008289" ] }, "pscc": { "uniref50_id": "UniRef50_P75312", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75312" ], "product": "DegV domain-containing protein MG326 homolog" }, "genes": [ "degV" ], "id": "APKECPOBEN_1028", "locus": "GCA002090215_01024" }, { "type": "cds", "sequence": "contig_1", "start": 575199, "stop": 576005, "strand": "-", "frame": 2, "gene": "menH", "product": "Putative esterase/lipase 2", "db_xrefs": [ "COG:COG0596", "COG:HR", "EC:3.1.-.-", "GO:0016020", "GO:0052689", "RefSeq:WP_010874829.1", "SO:0001217", "UniParc:UPI000012A1B6", "UniRef:UniRef100_P75311", "UniRef:UniRef50_P75311", "UniRef:UniRef90_P75311" ], "nt": "ATGGCAAGCAGTAAGAGCGATCCCTTTCAATCAATCTTTGCCTTTAAACCACACCGTAAGCGCCATAACTTTATCTTTCTCCATGGTTTTGGCAGTGAGTATTCCAGCTTTAAACACGTGTTTAAACTGTTTGAAAAAAAGCGCTGGTCGTTCTTTGCCTTTAATTTTCCGGGACACGGCAATAATCAGAGTAACAGTGTTGATGAATTGAAGTTGAAACACTATGTTGAATTGGTGTGTGACTTTATCATCCAAAAGCGCTTGAAAAAGGTGGTATTGGTAGGGCACAGTATGGGCGGGGCGATTGCGGTGTTGGTCAATGCTGTATTGCGTGAACGCATTAAGGCCTTAGTGTTGGTAGCACCGATGAATCAAACATCGTTTGTTGTAAGTAAAAAACGGATCTTGGATACCTTGTTTACCAGGAGTCCTAAAAACCAGCAGGACTTCATAGAACACACCGATGATAAGAAGTCGATTGTTAACTTCTTTGTTGGTGCTTTTAAGAAGCGAGTCAACTTTAAAACACTTTACAGTGACATGGTGCAAAACGCCAAGTATGGTAATGATTATCTCGAAGCGGGTTACAACGCAATTAAGGACAAACCCACCTTAGTGGTTCTCGGTTCCAATGATATTGTCACCCCCACAAAGGCTTCTGTGGAATACTTAGCTAAACACAGCGAAACGATCATTTTCAAAATTATTGATGGTGTAGGACATTCACCCCACTACTATGCACCCAAACTTTTTTTTGATTACATTGGGGAGTTTCTAGATAATATAAAGCGAAATAAGGACAAATAG", "aa": "MASSKSDPFQSIFAFKPHRKRHNFIFLHGFGSEYSSFKHVFKLFEKKRWSFFAFNFPGHGNNQSNSVDELKLKHYVELVCDFIIQKRLKKVVLVGHSMGGAIAVLVNAVLRERIKALVLVAPMNQTSFVVSKKRILDTLFTRSPKNQQDFIEHTDDKKSIVNFFVGAFKKRVNFKTLYSDMVQNAKYGNDYLEAGYNAIKDKPTLVVLGSNDIVTPTKASVEYLAKHSETIIFKIIDGVGHSPHYYAPKLFFDYIGEFLDNIKRNKDK", "aa_hexdigest": "77a8a3ac89beaa0abf5b2a128db2a15a", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000012A1B6", "ncbi_nrp_id": "WP_010874829.1", "uniref100_id": "UniRef100_P75311", "db_xrefs": [ "SO:0001217", "UniParc:UPI000012A1B6", "RefSeq:WP_010874829.1", "UniRef:UniRef100_P75311" ] }, "ips": { "uniref100_id": "UniRef100_P75311", "uniref90_id": "UniRef90_P75311" }, "psc": { "uniref90_id": "UniRef90_P75311", "gene": "MenH", "product": "Putative esterase/lipase 2", "ec_ids": [ "3.1.-.-" ], "uniref50_id": "UniRef50_P75311", "cog_id": "COG0596", "cog_category": "HR", "go_ids": [ "GO:0016020", "GO:0052689" ] }, "pscc": { "uniref50_id": "UniRef50_P75311", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75311" ], "product": "Putative esterase/lipase 2" }, "genes": [ "menH" ], "id": "APKECPOBEN_1029", "locus": "GCA002090215_01025" }, { "type": "cds", "sequence": "contig_1", "start": 575992, "stop": 579414, "strand": "-", "frame": 1, "gene": "smc", "product": "putative MG328-like protein", "db_xrefs": [ "COG:COG1196", "COG:D", "RefSeq:WP_041359968.1", "SO:0001217", "UniParc:UPI0003043689", "UniRef:UniRef100_P75310", "UniRef:UniRef50_P75310", "UniRef:UniRef90_P75310" ], "nt": "ATGACAGCAGAAAAAGAGCTAGGTATCCACGATTTTGACGAAAACTTAAACGAACAAAGTTTGCTCAAGCAACTCATGCCCAAAACCCGTAACATTACCCTAGAACCAACAAGGGAAACAATAACAGTTCCCCTAGAACGTAACATTGTAGAACGTAGTTTCTTTGGCGAATTAGATCAGTTAGAGAAAGAACAATCAATTCGCAATTTTTTTCACTTTATGGTAGATATGGGTGTGGTCAAAAGCCAACCCCATGTTGATGTGTTAAACCACTTCGCTAACCAACTCCAAGTTCATAAACCGCAAACCGTTGATCTAACAATGGAGTTTTTAGAACAAGAAGGCCAGGAAGTACTAACAAAGGAAATTAAGGCTGGTTTTTGTGAAATTACCCCCTCTAGTATTACCGAACAAACGACCAAGCCCCAGTTAGATGAAACTCAACTAGTCGATGAATACGTGCACACCAAAGAGTTAGAAACAACACCAATACCTATATCCTTTGCCACTAAGGAAGTACTGTTTGAAGAGGTCTTTAATACTCCTTCAACCCAACAAGTCGATGAAAGTGTGCTTGTCAACGAATACATTGAACTAACCCAACAAATCAAAAACGCATCAGAACAAGTTTCATCAAACCATACCCATAAGTTTAGTGTGGCAACAGAACCAGCAGCCACAAAAGCTGTTAGTGAAACTATGTTGTTGGATGACTATGTCGAAATGGTGGAACAGGATGTTCAAGCCCAAACTGCTTTACCCCAAGCAGCACTTGATCCTACTGTTAGCTTGACCTTTAGCAGTCCGATTGACAGTAATGCCATTTTGGTGTACCCGGAAATGAAGGTACCTCATGTATTTGACACAGTTGCACCAACTACCACAACTGTTCCGTTAGACCAAACACAACTGCTTGACGAACTAGTGGAAGTGCCAGTTTTAACTCACACTGTAACTCCAGCACCGTTACAACCAAAAGCAGCACCAACTAACTTTGCACTTGACCAAACCCAGTTAGTCGATGAATTAGTAACAGTACCACTCACCCATACATTAGTCAACGAGTCTGCACCAGTTACACCTGTTGTTGTGACATCACCTGCAGCTGAACATAGCTTCTCGATTACAACGGTGGACAAGGCTAATTTAACTAATGCTTTGAGCCAAACAGTGGTTATAAAACCCGCTGAAGACAGCGCTCATCAAAGTGCCGTTTTAGACAAGGAAATTGCCACCAAACAAGCGCAGTTACAACAATTGCAAGCCCAAATTGAACTACGCCAAGCTCAACTAGAAACACCTCCTGTAACTTACATGGGTGTGGAAGAATACAAGCTACTGCCTGTTCAAGATGTAGTGCCAGTACAACCTACCGTGAGTTTTGAAATGACCCTGCTCCAAGAACAGTTAGATAAGGCCTTGAAGCACAACGCTGCTTTACAAATCCAGCTAGAAGAGCAGTTAGCAAAGCCACTTCAGTACGACCAAAGTCCGGTGTTACAGGAACGGATTGAACTGTTGCAAAACCAAAATACTAACCTCACTCAGGAGTTGAATGAACTGCAACAAAAGCTGTTTAAGAGCCAAAACAACAGCTTACTCTTAGCACGTTTGGAAGAGGAAAACCGTACGCTTAAACAGCATTTACAAAACAACCTACCGGAAGCTAACCAGTTAAACTTTGTCTTGGAAAAACAGCTTGAACAACTCCAACAAGACAAACACAGCTTAACACTCCAAATCGAACAGTACAAGTTTGATTCCAAAAAACACCAGGAACAACTGGCTTTAATTCCGTCGTTACGCAGTGAAATTAACAGTTTGGAAACGGAAGTAATTAGCTTAAAGCAAACTAACCAACGCTTAAGCCTAATTGAGCGTGAAAACAACTTCCTCAAAACCGAAATTAAACAGCTACGTGAAACTAAGTTAAACGATGAAAACACCAAGTACCGTAATCTTTTAAAGCAGTATGAGTTAATGCGTGCTGACAGTGATGCTAAGTTAAAGGAATTGGAACACGAACAACACCTAGCACACCAACACCACCAGGAACAGTTAGCACAATTACAACGCCACAACGAAGCTTTAGTAAAAGAGTTAGACCAAGTTAAGGCCACTAACTTTGAGTTAGGTCTCGCAGCACAAGGTTTTGAACAGCAAAAAGTGGTACTAGAACAAAAAAACAGCAGTTTACTTGCAAGCTTACAAGCAGCTGAGGAAAACGTCCAGGCCTTGGGTATTACTAACAGTGAACTACAAAACCAACTAAATGTTTTAGAATTTACCCACAAGGAAAAAACTGCGTTTGACAGCAAAACGCTAACACTCACAAAGCAACAGCTAGAACAAACCCAGTTTGACCTTAGCTTAACCCAAGAACAGTTAGCTACCTTCAAACAACAAAACCAAAGCCTAACTGACAAACTAATGGCTAGCGAAACCCAGTTAAACCACTTGCAACAGAGTGATGAAAACTTAACCCAGCTCCAAACGCAGCACGAGCTGTTGCAAGAGAGTTACAACAAATTGCAAGATGAAGCTAATCACACCCAGCAACAATTCCATCAAGCACAAAATGAACTTGATGCTGCCCACCAACAGTTAGCACTCTTTAAGCAAAACAACGAGGAATTAACTGATAAATGCAGTAACATTCAAAATGAGTTGCATGATTTAAACCGGGTAAAGACTAACTGGGAGAACCTCAATACAGAGCATAACTTGTTGCAAGACAAATATGCTCAACAAAAAGAGCAAATGCAACATGAACACAGTAATTTAGCCCAAATCCAAGCGGAGCATGAGTTACTGCAAGAGAGTTACAACAAGGTCAAAGCAGAATTAAACGAAATTCAAATTACTAACCTTAATGAGGCCAACGCCCAGTACCAGGACTTGCTCTCCGCTTACGAGTTGTTACAAAGTAATCACAACAAGCTCAAGCAAGAGTTGCAAGTTTTAAACCAGGTCAACTTGGAAAAACAACAGTTAGCGCAAAAGTTACACAACACACACCAAAGTTTAAGTCAAACCCATGCAGAGCTAACCCAACTCCAAGCTGCTTACAATAACCTCCAAGCAACTCCACCGGTAAGCGATGAATTGTTAGAACAATTTAACCAAGTGCAGCTAGAAAAACAACGCTTACTGCAACAAAACTTAGCCTTAGTACACGAGTTGCAATACTTCAACGAACTCAACTCGAGTCAAACCCACGAAATTAAAACAAAGCAAGACGAAACTGTTAAAGAGGTCATTATTGTGGAAAAGGAAATTCCAGTGCCACCCGAAAAGAAACCACGGCTAAAAAAACGTGATATTGTGATCGAAAACAAGGAAGATGCTTTGGGCAAGTTATCCAAAAAGGAACGGATCCAAGCGTACGCTGAACGCCTAGCTAAGATCAATGGCAAGCAGTAA", "aa": "MTAEKELGIHDFDENLNEQSLLKQLMPKTRNITLEPTRETITVPLERNIVERSFFGELDQLEKEQSIRNFFHFMVDMGVVKSQPHVDVLNHFANQLQVHKPQTVDLTMEFLEQEGQEVLTKEIKAGFCEITPSSITEQTTKPQLDETQLVDEYVHTKELETTPIPISFATKEVLFEEVFNTPSTQQVDESVLVNEYIELTQQIKNASEQVSSNHTHKFSVATEPAATKAVSETMLLDDYVEMVEQDVQAQTALPQAALDPTVSLTFSSPIDSNAILVYPEMKVPHVFDTVAPTTTTVPLDQTQLLDELVEVPVLTHTVTPAPLQPKAAPTNFALDQTQLVDELVTVPLTHTLVNESAPVTPVVVTSPAAEHSFSITTVDKANLTNALSQTVVIKPAEDSAHQSAVLDKEIATKQAQLQQLQAQIELRQAQLETPPVTYMGVEEYKLLPVQDVVPVQPTVSFEMTLLQEQLDKALKHNAALQIQLEEQLAKPLQYDQSPVLQERIELLQNQNTNLTQELNELQQKLFKSQNNSLLLARLEEENRTLKQHLQNNLPEANQLNFVLEKQLEQLQQDKHSLTLQIEQYKFDSKKHQEQLALIPSLRSEINSLETEVISLKQTNQRLSLIERENNFLKTEIKQLRETKLNDENTKYRNLLKQYELMRADSDAKLKELEHEQHLAHQHHQEQLAQLQRHNEALVKELDQVKATNFELGLAAQGFEQQKVVLEQKNSSLLASLQAAEENVQALGITNSELQNQLNVLEFTHKEKTAFDSKTLTLTKQQLEQTQFDLSLTQEQLATFKQQNQSLTDKLMASETQLNHLQQSDENLTQLQTQHELLQESYNKLQDEANHTQQQFHQAQNELDAAHQQLALFKQNNEELTDKCSNIQNELHDLNRVKTNWENLNTEHNLLQDKYAQQKEQMQHEHSNLAQIQAEHELLQESYNKVKAELNEIQITNLNEANAQYQDLLSAYELLQSNHNKLKQELQVLNQVNLEKQQLAQKLHNTHQSLSQTHAELTQLQAAYNNLQATPPVSDELLEQFNQVQLEKQRLLQQNLALVHELQYFNELNSSQTHEIKTKQDETVKEVIIVEKEIPVPPEKKPRLKKRDIVIENKEDALGKLSKKERIQAYAERLAKINGKQ", "aa_hexdigest": "8c7b0bdf767a8076fd93a548c00d7d65", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0003043689", "ncbi_nrp_id": "WP_041359968.1", "uniref100_id": "UniRef100_P75310", "db_xrefs": [ "SO:0001217", "UniParc:UPI0003043689", "RefSeq:WP_041359968.1", "UniRef:UniRef100_P75310" ] }, "ips": { "uniref100_id": "UniRef100_P75310", "uniref90_id": "UniRef90_P75310" }, "psc": { "uniref90_id": "UniRef90_P75310", "gene": "Smc", "product": "Uncharacterized protein MG328 homolog", "uniref50_id": "UniRef50_P75310", "cog_id": "COG1196", "cog_category": "D" }, "pscc": { "uniref50_id": "UniRef50_P75310", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75310" ], "product": "Uncharacterized protein MG328 homolog" }, "genes": [ "smc" ], "id": "APKECPOBEN_1030", "locus": "GCA002090215_01026" }, { "type": "cds", "sequence": "contig_1", "start": 579451, "stop": 579873, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF14714.11" ], "nt": "ATGGCTAAGTTTAAAAAGCAGTTACAAAGTCAGTTTCAACACCTCAGCTTTGCTCCCATTGTTTTTATTAGTGTCAAAAACAACAAGCGCTTACATACCATCTTTGAACAGCTCCAAATTATCCAGGAACAGCTCACCAAAAAGATTTCAACTTCCCTGTTAAACGATGTCATCCAACAAGCACAACTGTTTAACCAAGCACCCCTGTTTAAGGGTGGGAGGTTACAGGTAACTTATGCGGTGCAAACTCACAGTCAAACGCCGCACTTTGTACTGTTCTGTAATGACCCTAAGTTTGTCCACTTTTCCTATGCGCGCTTTTTAGAGAACAAGATTAGGGAAAGCTTTGGTTTTAGTGCTGTACCTATTACTCTTTATTTCAAGTCCAAAAATGCACGGATAAGAGGTGTCGCAAAAACCTAA", "aa": "MAKFKKQLQSQFQHLSFAPIVFISVKNNKRLHTIFEQLQIIQEQLTKKISTSLLNDVIQQAQLFNQAPLFKGGRLQVTYAVQTHSQTPHFVLFCNDPKFVHFSYARFLENKIRESFGFSAVPITLYFKSKNARIRGVAKT", "aa_hexdigest": "baa0285819b27dedec0c8312a740e54d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF14714.11", "name": "KH_dom-like", "length": 81, "aa_cov": 0.5785714285714286, "hmm_cov": 1.0, "evalue": 3.515034367672489e-31, "score": 102.84827423095703, "start": 48, "stop": 128 } ], "seq_stats": { "molecular_weight": 16233.708699999988, "isoelectric_point": 10.332235145568848 }, "id": "APKECPOBEN_1031", "locus": "GCA002090215_01027" }, { "type": "cds", "sequence": "contig_1", "start": 579901, "stop": 580800, "strand": "-", "frame": 1, "gene": "der", "product": "ribosome biogenesis GTPase Der", "db_xrefs": [ "COG:COG1160", "COG:J", "EC:3.6.5.-", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0042254", "GO:0043022", "KEGG:K03977", "SO:0001217", "UniRef:UniRef50_P75309", "UniRef:UniRef90_P75309" ], "nt": "GTGTTTACTGTTGCCATTATCGGCCGACCTAACGTTGGCAAATCGAGTTTGTTCAATCGCTTAATCCAAAAACCCTACGCGATTATTTCCGATACGCCCAATACCACAAGGGACCGCATTTATGGTGTTGGTGAATGGTTAACGCGGCAAATTGCCTTTATTGATACTGGGGGATTAATTTCCCAAAAGACCCCGCTCCAACAACAAATTGAAGTCCAAGTTCGAGCAGCCTTAAGTCAAGCTAATGCGATTATCTTCTTGGTGTCTTACCAAGAACAGATTAGCAGTGATGACTTTTATGTAGCTAAGGTATTGAAAAAGATTAAGGACAAACCGATTTTGTTGGTCGTCAATAAATCAGAAAACCTCAAACCAGATGCTTATGAACCCAACCTACAACAGTTCTACAGCTTTGGGTTTGGCCAACCCGTTTGTGTGAGTGCTAGCCATGGCATTGGCATTGGTAACCTAATGGATCGACTAGTTAAGGACAACCAACTCCCCCCTTACCATGGTAGTAGTGAAACCAATCCCGAAGTGCGCTTTTGTGTAATTGGTAAACCCAATGTTGGCAAGAGTTCTTTAATTAACCAACTGGTGCAGCAAAACCGGGTGTTAGTGTCCGATGAGAGTGGTACTACCCGTGATGCAATTGATATTCCCTTAAGGGTTAATGGGCAAAACTACCTTTTAATTGATACGGCTGGGATTCGCCGTAAGGGGAAAATAGCCCCCGGAATTGAAGCGGCCTCGTATGGGAAAACCCAGTTAGCAATCGCTAGGAGCAACATTATTCTGTTAATGGTTGATGGTTCCAAACCGTTGAGTGAACAGGACGAGATTATCGGTGGGTTAGCCCAAGCCGCTTTAATTCCGGTGATTATTTTGGTCAACAAATGA", "aa": "MFTVAIIGRPNVGKSSLFNRLIQKPYAIISDTPNTTRDRIYGVGEWLTRQIAFIDTGGLISQKTPLQQQIEVQVRAALSQANAIIFLVSYQEQISSDDFYVAKVLKKIKDKPILLVVNKSENLKPDAYEPNLQQFYSFGFGQPVCVSASHGIGIGNLMDRLVKDNQLPPYHGSSETNPEVRFCVIGKPNVGKSSLINQLVQQNRVLVSDESGTTRDAIDIPLRVNGQNYLLIDTAGIRRKGKIAPGIEAASYGKTQLAIARSNIILLMVDGSKPLSEQDEIIGGLAQAALIPVIILVNK", "aa_hexdigest": "5ea8883a405ca73f4a52e9ab9e63f8cc", "start_type": "GTG", "rbs_motif": null, "genes": [ "der" ], "truncated": "5-prime", "pseudogene": { "start": 579598, "stop": 580800, "observations": { "mutation": [ 579903 ], "stop": [ 579903 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75309", "query_cov": 1.0, "subject_cov": 0.6659242761692651, "identity": 1.0, "score": 582.0, "evalue": 1.92e-207, "gene_start": 1, "gene_end": 299, "reference_start": 1, "reference_end": 299, "reference_sequence": "MFTVAIIGRPNVGKSSLFNRLIQKPYAIISDTPNTTRDRIYGVGEWLTRQIAFIDTGGLISQKTPLQQQIEVQVRAALSQANAIIFLVSYQEQISSDDFYVAKVLKKIKDKPILLVVNKSENLKPDAYEPNLQQFYSFGFGQPVCVSASHGIGIGNLMDRLVKDNQLPPYHGSSETNPEVRFCVIGKPNVGKSSLINQLVQQNRVLVSDESGTTRDAIDIPLRVNGQNYLLIDTAGIRRKGKIAPGIEAASYGKTQLAIARSNIILLMVDGSKPLSEQDEIIGGLAQAALIPVIILVNKWDLVQKDSNTMAKFKKQLQSQFQHLSFAPIVFISVKNNKRLHTIFEQLQIIQEQLTKKISTSLLNDVIQQAQLFNQAPLFKGGRLQVTYAVQTHSQTPHFVLFCNDPKFVHFSYARFLENKIRESFGFSAVPITLYFKSKNARIRGVAKT" }, "paralog": false, "identity": 0.995, "score": 773.0, "evalue": 4.3e-285, "description": "Internal stop codon at 579903. Nonsense mutation around 579903.", "psc": { "uniref90_id": "UniRef90_P75309", "gene": "der", "product": "ribosome biogenesis GTPase Der", "ec_ids": [ "3.6.5.-" ], "uniref50_id": "UniRef50_P75309", "cog_id": "COG1160", "cog_category": "J", "kegg_orthology_id": "K03977", "go_ids": [ "GO:0003924", "GO:0005525", "GO:0005737", "GO:0042254", "GO:0043022" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75309", "EC:3.6.5.-", "UniRef:UniRef50_P75309", "COG:COG1160", "COG:J", "KEGG:K03977", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0042254", "GO:0043022" ] }, "pscc": { "uniref50_id": "UniRef50_P75309", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75309" ], "product": "GTPase Der" } }, "id": "APKECPOBEN_1032", "locus": "GCA002090215_01028" }, { "type": "cds", "sequence": "contig_1", "start": 580802, "stop": 581455, "strand": "-", "frame": 3, "gene": "cmk", "product": "(d)CMP kinase", "db_xrefs": [ "COG:COG0283", "COG:F", "EC:2.7.4.25", "GO:0004127", "GO:0005524", "GO:0005829", "GO:0006220", "GO:0015949", "GO:0036430", "GO:0036431", "RefSeq:WP_010874832.1", "SO:0001217", "UniParc:UPI000012DCF0", "UniRef:UniRef100_P75308", "UniRef:UniRef50_P47572", "UniRef:UniRef90_P75308" ], "nt": "ATGTATTTTCAAATTGCCATTGACGGTCCTAGTAGTTCTGGTAAATCTAGTGTTGCTAAAACAGTGGCCCGACAGCTCGGTTTTGAGTACTTTTCCACTGGCAAAATGTACCGGGCCTTCGCTTACGTGATGCAGGTCAACCGCTTGGATGTCAACCTTTTGTTAAAGGTGATTAACCAAATTAACTGGCGCTTTGAACACGAAAAGGTGTTTTATAACAACGCTGATATCTCAGAGGTTATCCTAAACCAGGAAATTGCCCAACTAGCGAGCAATTTAGCAACCAATCCCGAAGTGCGTAAGATGGCGGTATTGCGCCAACAAGCGTTAGCTAAAAACACCAACATTGTGATGGATGGACGCGACATTGGTACCGTTGTATTGAAGGACGCGCAGTTAAAGTACTTCTTGGATGCTAAACCGGAAATAAGAGCGCAACGACGCGCCCAAGATCTTGGCATAGCATATGACAGTGACAAGGCCTTTCAGGAACTAGTAGCGGAAATTAAGCACCGTGATGCGGTGGATACTTCCAGAACTGCTGATCCCTTAGTGCAAGCCCCCGATGCGATTTACATCGACAGTTCTAACCTTACTTTTCAGCAAGTAGTCGAGTTAATGGTGCAACAAGCGCGTACAGTTTTTAAACTTTAA", "aa": "MYFQIAIDGPSSSGKSSVAKTVARQLGFEYFSTGKMYRAFAYVMQVNRLDVNLLLKVINQINWRFEHEKVFYNNADISEVILNQEIAQLASNLATNPEVRKMAVLRQQALAKNTNIVMDGRDIGTVVLKDAQLKYFLDAKPEIRAQRRAQDLGIAYDSDKAFQELVAEIKHRDAVDTSRTADPLVQAPDAIYIDSSNLTFQQVVELMVQQARTVFKL", "aa_hexdigest": "2ab2763f60287f23f9a9edc9ce80afdf", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000012DCF0", "ncbi_nrp_id": "WP_010874832.1", "uniref100_id": "UniRef100_P75308", "db_xrefs": [ "SO:0001217", "UniParc:UPI000012DCF0", "RefSeq:WP_010874832.1", "UniRef:UniRef100_P75308" ] }, "ips": { "uniref100_id": "UniRef100_P75308", "uniref90_id": "UniRef90_P75308" }, "psc": { "uniref90_id": "UniRef90_P75308", "gene": "cmk", "product": "(d)CMP kinase", "ec_ids": [ "2.7.4.25" ], "uniref50_id": "UniRef50_P47572", "cog_id": "COG0283", "cog_category": "F", "go_ids": [ "GO:0004127", "GO:0005524", "GO:0005829", "GO:0006220", "GO:0015949", "GO:0036430", "GO:0036431" ] }, "pscc": { "uniref50_id": "UniRef50_P47572", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47572" ], "product": "Cytidylate kinase" }, "genes": [ "cmk" ], "id": "APKECPOBEN_1033", "locus": "GCA002090215_01029" }, { "type": "cds", "sequence": "contig_1", "start": 581482, "stop": 581991, "strand": "-", "frame": 1, "gene": null, "product": "putative MG331-like protein", "db_xrefs": [ "GO:0016020", "SO:0001217", "UniRef:UniRef50_P75307", "UniRef:UniRef90_P75307" ], "nt": "TTGAGCCCGAAAATTCTGTCGCACTACCAGCCTTTTAGTGAATTGAGTGCTAATCCGGTGAAACGCAAAAGTGAACCCAATCAGGTCTTACAATCACTGCAAACTCTCATTAACAACTTCCAAACATCGGATTTTCAAGCCATCGCCACCCAAGCTGATGCCTTGTGGTTACAAATTAAACACCACGATAGCGCGACTGGTTATGAAAGTTGGATTACTGATGACCGGGGGATGGATAAAATAGATAACCTAAAGCGCTTTTTTGAGGACAACGAACAACAGTTTATTCAGCAAACGCAAACTTTCGTAGTGCAATTGGGGAACTATAATAAGGCACTAATGGCACTCACTAACGAAAAAGTGAGCTTTACCACCACTGATTTAAGCCAGCCCTTAGCGAGCAGTTACAACCCGTTATTTAAGCGGATTTTGCTCAGTTTATTAGTATTCTTTATTAGTAGTTTAGTGGGTTTGGTTGTTTTACTAACCCTCTTATTCACTTCCCTTTAA", "aa": "MSPKILSHYQPFSELSANPVKRKSEPNQVLQSLQTLINNFQTSDFQAIATQADALWLQIKHHDSATGYESWITDDRGMDKIDNLKRFFEDNEQQFIQQTQTFVVQLGNYNKALMALTNEKVSFTTTDLSQPLASSYNPLFKRILLSLLVFFISSLVGLVVLLTLLFTSL", "aa_hexdigest": "b5b40653b8a922273dadba5fbd55fb84", "start_type": "TTG", "rbs_motif": "AAAA", "psc": { "uniref90_id": "UniRef90_P75307", "query_cov": 1.0, "subject_cov": 0.8086124401913876, "identity": 0.9940000000000001, "score": 315.0, "evalue": 9.49e-108, "valid": true, "product": "Uncharacterized protein MG331 homolog", "uniref50_id": "UniRef50_P75307", "go_ids": [ "GO:0016020" ] }, "pscc": { "uniref50_id": "UniRef50_P75307", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75307" ], "product": "Uncharacterized protein MG331 homolog" }, "genes": [], "id": "APKECPOBEN_1034", "locus": "GCA002090215_01030" }, { "type": "cds", "sequence": "contig_1", "start": 582010, "stop": 582111, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGACGCTTAGAGAAGTTTCGCTTTTACCGTCAGAACGATGACAATAATACGACTGTTCAAAAAGCGTTACTGAAAGCGCAAAAACAGGTCGCTAGTTGA", "aa": "MGRLEKFRFYRQNDDNNTTVQKALLKAQKQVAS", "aa_hexdigest": "cd0a4e426ba0cf40f9ec792e15d2b977", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3899.396700000001, "isoelectric_point": 10.160878181457516 }, "id": "APKECPOBEN_1035", "locus": "GCA002090215_01031" }, { "type": "cds", "sequence": "contig_1", "start": 582114, "stop": 582821, "strand": "-", "frame": 2, "gene": "tACO1", "product": "putative transcriptional regulatory protein MPN_478", "db_xrefs": [ "COG:COG0217", "COG:KJ", "GO:0003677", "GO:0005829", "GO:0006355", "RefSeq:WP_010874834.1", "SO:0001217", "UniParc:UPI000013963C", "UniRef:UniRef100_P75306", "UniRef:UniRef50_P47574", "UniRef:UniRef90_P75306" ], "nt": "ATGCCGCGGAAGCATTTAATTGCCAGTCAAACAAACAAAAAACAACAATCCAACGCGAAGCAACTACAAAAGTTAGCGAAGCGGATCGCTGCTGCTGTCAAAAAAGGTGGCAGTAACATTGATGCCAACCCCCAGCTAAAGGTGGCAGTCGAACTAGCGTTAGCTCATGGTTTAAGTGCGGATTCAATTAAACGCAACATCCATGGCAGTGAAAAGGATCCCACAAAACTAAGCGAGTTTTGCTATGAGATCTTTGGTCCCAATGGTGTTGGCATCATTGTCTTTGGTCTAACCGATAATCCGAACCGGTTGTTGAGTAGTTTAAACGGTTACATTGCTAAATTAAAGGCACAGCTAGCCAAGCCCAACAGTGTTAAAATTAACTTTGAGGAAAAGGGCATTGCGCTAGTTAAACACAATAACTTTACCCAAGACGAGTTAATTGAACTGTTAATTAGTAACAACATTAATTTGTTAGATCTCAATGAAGATGATGACAGCTTTGAAGTAGTGGTCGATTCACCGAGTTACTTTGCGTTGAAGGATTTACTCGTTAAAAACAGTTTTACGATTGAAGCGAGTGAGTTGCGCTTAATTCCGCTGTTAACGGTGGAACTTAACGCCGAACAACACACCCTCTTAAACCGCTTTTTAAATGCGTGTGAAGAGGATGATGACATCCAAACAGTAGTGCATAATGCACTGTAG", "aa": "MPRKHLIASQTNKKQQSNAKQLQKLAKRIAAAVKKGGSNIDANPQLKVAVELALAHGLSADSIKRNIHGSEKDPTKLSEFCYEIFGPNGVGIIVFGLTDNPNRLLSSLNGYIAKLKAQLAKPNSVKINFEEKGIALVKHNNFTQDELIELLISNNINLLDLNEDDDSFEVVVDSPSYFALKDLLVKNSFTIEASELRLIPLLTVELNAEQHTLLNRFLNACEEDDDIQTVVHNAL", "aa_hexdigest": "94cd58cb630adeb32042c4dea2725291", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013963C", "ncbi_nrp_id": "WP_010874834.1", "uniref100_id": "UniRef100_P75306", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013963C", "RefSeq:WP_010874834.1", "UniRef:UniRef100_P75306" ] }, "ips": { "uniref100_id": "UniRef100_P75306", "uniref90_id": "UniRef90_P75306" }, "psc": { "uniref90_id": "UniRef90_P75306", "gene": "TACO1", "product": "Probable transcriptional regulatory protein MPN_478", "uniref50_id": "UniRef50_P47574", "cog_id": "COG0217", "cog_category": "KJ", "go_ids": [ "GO:0005829", "GO:0003677", "GO:0006355" ] }, "pscc": { "uniref50_id": "UniRef50_P47574", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47574" ], "product": "Probable transcriptional regulatory protein MG332" }, "genes": [ "tACO1" ], "id": "APKECPOBEN_1036", "locus": "GCA002090215_01032" }, { "type": "cds", "sequence": "contig_1", "start": 582999, "stop": 583418, "strand": "-", "frame": 2, "gene": "azoR", "product": "FMN-dependent NADH:quinone oxidoreductase", "db_xrefs": [ "COG:C", "COG:COG1182", "EC:1.6.5.-", "GO:0009055", "GO:0010181", "GO:0016652", "GO:0016655", "KEGG:K01118", "SO:0001217", "UniRef:UniRef50_P47575", "UniRef:UniRef90_P75305" ], "nt": "ATGAAGAAGGTAATTATTATTGATGCTTCTGTATCACCTAGTGGTTCTTACACCCATTTATTGTTAGAACGCTTTTTAGCTACCTTTCAAGCTAAAAACAAGGACGTAGAGCTCTCCACTTGGAACCTCAACGAACTACCAGTGGGACAAATTTCCTATAACACCCAAAACGCTGGAACTTTCTTTTCTGTAGAAAACTCCGATAAGTACATTGATGCGCTCAAAGCAGCGCACGGTGTTATTATCTTGGCGCCAATGACCAACTTTAACTACCCCGCAACGTTAAAGAACTTTATTGACCATGTCTTTGTGGCAAACAAAACCTTTAAGGATAAGTATGTCACTAAGGGCGCATCATCAGGAATGTTGGGTAACCTCAAGGTAGTCGTTTTGGGTTCCCAAGGCGCACCGTTAGGTTGA", "aa": "MKKVIIIDASVSPSGSYTHLLLERFLATFQAKNKDVELSTWNLNELPVGQISYNTQNAGTFFSVENSDKYIDALKAAHGVIILAPMTNFNYPATLKNFIDHVFVANKTFKDKYVTKGASSGMLGNLKVVVLGSQGAPLG", "aa_hexdigest": "c15fd1d27fece49966a775d820ee2fcf", "start_type": "ATG", "rbs_motif": "AATAA", "genes": [ "azoR" ], "truncated": "5-prime", "pseudogene": { "start": 582825, "stop": 583418, "observations": { "mutation": [ 583001 ], "stop": [ 583001 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75305", "query_cov": 1.0, "subject_cov": 0.7055837563451777, "identity": 1.0, "score": 275.0, "evalue": 6.42e-93, "gene_start": 1, "gene_end": 139, "reference_start": 1, "reference_end": 139, "reference_sequence": "MKKVIIIDASVSPSGSYTHLLLERFLATFQAKNKDVELSTWNLNELPVGQISYNTQNAGTFFSVENSDKYIDALKAAHGVIILAPMTNFNYPATLKNFIDHVFVANKTFKDKYVTKGASSGMLGNLKVVVLGSQGAPLGWYPWGDHVNSLRGLFGFAGVASFASVIIDGTKLLYKDKSKSEVVDMFAKQVDAIANEF" }, "paralog": false, "identity": 0.9949238578680203, "score": 388.0, "evalue": 9.89e-140, "description": "Internal stop codon at 583001. Nonsense mutation around 583001.", "psc": { "uniref90_id": "UniRef90_P75305", "gene": "azoR", "product": "FMN-dependent NADH:quinone oxidoreductase", "ec_ids": [ "1.6.5.-" ], "uniref50_id": "UniRef50_P47575", "cog_id": "COG1182", "cog_category": "C", "kegg_orthology_id": "K01118", "go_ids": [ "GO:0009055", "GO:0010181", "GO:0016652", "GO:0016655" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75305", "EC:1.6.5.-", "UniRef:UniRef50_P47575", "COG:COG1182", "COG:C", "KEGG:K01118", "GO:0009055", "GO:0010181", "GO:0016652", "GO:0016655" ] }, "pscc": { "uniref50_id": "UniRef50_P47575", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47575" ], "product": "FMN-dependent NADH:quinone oxidoreductase" } }, "id": "APKECPOBEN_1037", "locus": "GCA002090215_01033" }, { "type": "cds", "sequence": "contig_1", "start": 583446, "stop": 583964, "strand": "-", "frame": 2, "gene": null, "product": "class I tRNA ligase family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001EFE51D6", "UniRef:UniRef90_UPI001EFE51D6" ], "nt": "ATGCTAAGTACCGTTACTCCCTTCTTGGCGGAACGGATCTACCAACAGTTTCACAGTGGCAGTGTCATGCAAGCAAGTTGGCCAACTGCCAAAGCAGTTAAACCACCGAAACTGTTTGCTGATGTTGTTGAAGCCGTGTCCAGCTTGCGCCACTACAAGGCCAACAACCAACTAGTAGCTAACCAGAATTTAGCCGTTGTTTTAAGTGGTAAAGCTGCTCCTGTAGTGCAAAACTACTTCCACTTTAACTGGGTCGATCTTAGGATAGAAGTTAACAAAACTCCAGGCTTCCAAATCAAAATCGTTGACAATGCAGCCAACAACTTAGCCCACTTAGAAAAACAACGGAGCTTTTATTTAGCGGAAGTCCAACGTAGTCAAGCGATTACCACTAACCCTGCCTTTCTAAAAAAAGCACCACCGCACAAAGTTAAGGCGGAGCTTTTAAAGTTAGAGGAATATCAAAAAAAATTAGCGGAAGTCAATCATTTAATCGCTAAATTAACTAAAGCTGAGTAG", "aa": "MLSTVTPFLAERIYQQFHSGSVMQASWPTAKAVKPPKLFADVVEAVSSLRHYKANNQLVANQNLAVVLSGKAAPVVQNYFHFNWVDLRIEVNKTPGFQIKIVDNAANNLAHLEKQRSFYLAEVQRSQAITTNPAFLKKAPPHKVKAELLKLEEYQKKLAEVNHLIAKLTKAE", "aa_hexdigest": "b737cbd5f6bb5767da4910322969b259", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 583446, "stop": 584264, "observations": { "mutation": [ 584072 ], "stop": [ 584072 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001EFE51D6", "query_cov": 1.0, "subject_cov": 0.46112600536193027, "identity": 1.0, "score": 335.0, "evalue": 1.68e-113, "gene_start": 1, "gene_end": 172, "reference_start": 202, "reference_end": 373, "reference_sequence": "MPTDLLVTGYDILFFWVLRMTFNSYFQTKQLPFKQVLIHGLVRDAQNRKMSKSLNNGINPMDLIRDYGADATRLFLTSNHTPGDDLIFNEQKLKSAANFLNKLWNVTKYVLQLGEQAKTVPSTHLPSTLSERWIWAKLKQLIVQTTKLLDKYQLALANQALVNFIWNDFCNTFIETIKQEDTALLPQLYTTAKTVLSTAVVMLSTVTPFLAERIYQQFHSGSVMQASWPTAKAVKPPKLFADVVEAVSSLRHYKANNQLVANQNLAVVLSGKAAPVVQNYFHFNWVDLRIEVNKTPGFQIKIVDNAANNLAHLEKQRSFYLAEVQRSQAITTNPAFLKKAPPHKVKAELLKLEEYQKKLAEVNHLIAKLTKAE" }, "paralog": false, "identity": 0.9963235294117647, "score": 532.0, "evalue": 2.49e-193, "description": "Internal stop codon at 584072. Nonsense mutation around 584072.", "psc": { "uniref90_id": "UniRef90_UPI001EFE51D6", "product": "class I tRNA ligase family protein", "uniref50_id": "UniRef50_UPI001EFE51D6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001EFE51D6", "UniRef:UniRef50_UPI001EFE51D6" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001EFE51D6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001EFE51D6" ], "product": "class I tRNA ligase family protein" } }, "id": "APKECPOBEN_1038", "locus": "GCA002090215_01034" }, { "type": "cds", "sequence": "contig_1", "start": 584070, "stop": 584567, "strand": "-", "frame": 2, "gene": null, "product": "class I tRNA ligase family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001EFE51D6", "UniRef:UniRef90_UPI001EFE51D6" ], "nt": "GTGCCAACTGATCTTTTGGTAACCGGGTATGACATCCTCTTCTTTTGGGTCTTACGGATGACCTTTAACTCGTACTTTCAAACCAAGCAGTTACCTTTTAAACAAGTGTTAATTCACGGTCTAGTCCGCGATGCCCAAAACCGCAAAATGTCCAAGTCCCTAAACAACGGGATTAACCCAATGGACTTAATCCGTGATTATGGGGCCGATGCCACGCGCTTATTCTTAACTTCCAATCACACTCCGGGTGATGATTTAATCTTCAACGAACAGAAGTTAAAGAGTGCCGCTAACTTCTTGAACAAGTTGTGGAATGTGACCAAGTACGTGTTGCAGTTAGGAGAACAAGCCAAAACTGTACCAAGTACTCACTTACCCTCTACATTGAGTGAGCGCTGGATCTGGGCTAAATTGAAACAACTCATTGTCCAAACCACCAAGTTATTAGACAAGTACCAGTTAGCTTTGGCCAACCAAGCGTTAGTCAACTTTATTTGA", "aa": "MPTDLLVTGYDILFFWVLRMTFNSYFQTKQLPFKQVLIHGLVRDAQNRKMSKSLNNGINPMDLIRDYGADATRLFLTSNHTPGDDLIFNEQKLKSAANFLNKLWNVTKYVLQLGEQAKTVPSTHLPSTLSERWIWAKLKQLIVQTTKLLDKYQLALANQALVNFI", "aa_hexdigest": "0247efda14e376f468612d986773bbb0", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 583767, "stop": 584567, "observations": { "mutation": [ 584072 ], "stop": [ 584072 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001EFE51D6", "query_cov": 1.0, "subject_cov": 0.44235924932975873, "identity": 1.0, "score": 331.0, "evalue": 5.92e-112, "gene_start": 1, "gene_end": 165, "reference_start": 1, "reference_end": 165, "reference_sequence": "MPTDLLVTGYDILFFWVLRMTFNSYFQTKQLPFKQVLIHGLVRDAQNRKMSKSLNNGINPMDLIRDYGADATRLFLTSNHTPGDDLIFNEQKLKSAANFLNKLWNVTKYVLQLGEQAKTVPSTHLPSTLSERWIWAKLKQLIVQTTKLLDKYQLALANQALVNFIWNDFCNTFIETIKQEDTALLPQLYTTAKTVLSTAVVMLSTVTPFLAERIYQQFHSGSVMQASWPTAKAVKPPKLFADVVEAVSSLRHYKANNQLVANQNLAVVLSGKAAPVVQNYFHFNWVDLRIEVNKTPGFQIKIVDNAANNLAHLEKQRSFYLAEVQRSQAITTNPAFLKKAPPHKVKAELLKLEEYQKKLAEVNHLIAKLTKAE" }, "paralog": false, "identity": 0.9924812030075187, "score": 525.0, "evalue": 1.47e-190, "description": "Internal stop codon at 584072. Nonsense mutation around 584072.", "psc": { "uniref90_id": "UniRef90_UPI001EFE51D6", "product": "class I tRNA ligase family protein", "uniref50_id": "UniRef50_UPI001EFE51D6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001EFE51D6", "UniRef:UniRef50_UPI001EFE51D6" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001EFE51D6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001EFE51D6" ], "product": "class I tRNA ligase family protein" } }, "id": "APKECPOBEN_1039", "locus": "GCA002090215_01035" }, { "type": "cds", "sequence": "contig_1", "start": 584718, "stop": 585476, "strand": "-", "frame": 2, "gene": "valS", "product": "valine--tRNA ligase", "db_xrefs": [ "EC:6.1.1.9", "GO:0000166", "GO:0004832", "GO:0005524", "GO:0006438", "SO:0001217", "UniRef:UniRef50_UPI001EFEBE54", "UniRef:UniRef90_UPI001EFEBE54" ], "nt": "TTGTTTGAACAGGGCTTTATCTACCAAGCTGAAACCTTGGTTAACTGGGACACCAAGCTAAACACTGCCATCTCGAACATTGAAGTCATTAATAAACCAGTTGACCAACAGCTGTATTACATTGCTTATAAGTTAGCTAATAATCCCAAAAAACGTTTAGTGGTGGCAACTACTAGACCAGAAACGATCTTTGTTGATGTGTGTTTGTTTGTCCATCCCAAAGACAAGCACTACCACAGCTTTGTCAAACAAAAGGTAGTTAATCCGCTCACTGGTGCGTTAATGCCAGTGTTCACCGATAGTTATGTCGACAAGAAGTTTGGCACCGGTGTGTTGAAGTGCACCCCCGCCCATGACTTTAACGACTTTGCCTTAAACGAAAAGTACCGTTTACCGTTTGTAAGTTGCATTGACCACAATGGTTTGTTAAATGAGCACGCTAAGCAATTTACTGGTTTAACCGTAAGTGCAGCACGTCAGCAGGTAGTCGAGTTTTTACAAACGCAAAAACTACTAGTGAAAACTATGCCACTAACCAGTAACGTTGGTTTTTCCGAGCGCAGTGACACGGTCGTAGAACCGTTATTATCCAAGCAGTGGTTTGTCGATTTACCGAAACTCAAAAAAGCACTCGCCATTAAGAAATACCCCGAGTTAATTCCCAAGCGTTTTAACAAACAGGTAACAAGGTGGTTGAGTCAGCTCAAACCGTGGTGCATATCGCGCCAATTAATTTGGGGTCACCCTATTCCGGTGTGA", "aa": "MFEQGFIYQAETLVNWDTKLNTAISNIEVINKPVDQQLYYIAYKLANNPKKRLVVATTRPETIFVDVCLFVHPKDKHYHSFVKQKVVNPLTGALMPVFTDSYVDKKFGTGVLKCTPAHDFNDFALNEKYRLPFVSCIDHNGLLNEHAKQFTGLTVSAARQQVVEFLQTQKLLVKTMPLTSNVGFSERSDTVVEPLLSKQWFVDLPKLKKALAIKKYPELIPKRFNKQVTRWLSQLKPWCISRQLIWGHPIPV", "aa_hexdigest": "9f1bbed346d36710fa071d13504163d2", "start_type": "TTG", "rbs_motif": null, "genes": [ "valS" ], "truncated": "both", "pseudogene": { "start": 584500, "stop": 585776, "observations": { "deletion": [ 584645 ], "mutation": [ 584720, 585542 ], "stop": [ 584537, 584558, 584720, 585542 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001EFEBE54", "query_cov": 1.0, "subject_cov": 0.5163934426229508, "identity": 0.996, "score": 517.0, "evalue": 5.77e-182, "gene_start": 1, "gene_end": 252, "reference_start": 163, "reference_end": 414, "reference_sequence": "MDKQFSFQGQYDFKTVSTGLYDSWSSASFFKPQKNKVPFTAILPPPNLTGTLHIGHAFEVSITDQIMRFKRLRGYGVNWIPGFDHAGIATQTKYEKLARETNPEYFQAPRKQKVKMIMDWALTQGDTIQSQIKSLGASLNWNQVNFTLSKKASQIVNDSFIQLFEQGFIYQAETLVNWDTKLNTAISNIEVINKPVDQQLYYIAYKLANNPKKRLVVATTRPETIFVDVCLFVHPKDKHYHSFVKQKVVNPLTGALMPVFTDSYVDKKFGTGVLKCTPAHDFNDFALNEKYRLPFVSCIDHNGLLNEHAKQFTGLTVSAARQQVVEFLQTQKLLVKTMPLTSNVGFSERSDTVVEPLLSKQWFVDLPKLKKALAIKKYPELIPKRFNKQVTRWLSQLKPWCISRQLIWGHPIPVWTHKQSGALHVGSTAPTDKQNYTQSXHRCTRYLIFLITVTVNLFRLTQKQALCANWSFGNRVWHPLLLGLTDDL" }, "paralog": false, "identity": 0.9882629107981221, "score": 840.0, "evalue": 9.13e-312, "description": "Internal stop codon at 584537, 584558, 584720, 585542. Frameshift due to deletion around 584645. Nonsense mutation around 584720, 585542.", "psc": { "uniref90_id": "UniRef90_UPI001EFEBE54", "gene": "valS", "product": "valine--tRNA ligase", "ec_ids": [ "6.1.1.9" ], "uniref50_id": "UniRef50_UPI001EFEBE54", "go_ids": [ "GO:0000166", "GO:0004832", "GO:0005524", "GO:0006438" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001EFEBE54", "EC:6.1.1.9", "UniRef:UniRef50_UPI001EFEBE54", "GO:0000166", "GO:0004832", "GO:0005524", "GO:0006438" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001EFEBE54", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001EFEBE54" ], "product": "valine--tRNA ligase" } }, "id": "APKECPOBEN_1040", "locus": "GCA002090215_01036" }, { "type": "cds", "sequence": "contig_1", "start": 585540, "stop": 585785, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00133.28" ], "nt": "GTGAGTATCACTGACCAAATTATGCGCTTTAAGCGACTGCGGGGTTATGGCGTGAACTGGATTCCCGGATTTGACCATGCGGGGATTGCTACGCAAACCAAATACGAAAAGTTAGCACGGGAAACTAATCCCGAGTACTTCCAAGCGCCGCGTAAACAAAAGGTCAAAATGATTATGGATTGGGCCTTAACACAAGGGGACACCATTCAAAGTCAGATCAAAAGTTTAGGGGCATCCTTAAACTGA", "aa": "MSITDQIMRFKRLRGYGVNWIPGFDHAGIATQTKYEKLARETNPEYFQAPRKQKVKMIMDWALTQGDTIQSQIKSLGASLN", "aa_hexdigest": "d2307621c7038561392eb0d500ad24ab", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00133.28", "name": "tRNA-synt_1", "length": 80, "aa_cov": 0.9753086419753086, "hmm_cov": 1.0, "evalue": 6.729222141806672e-15, "score": 50.14482498168945, "start": 2, "stop": 80 } ], "seq_stats": { "molecular_weight": 9319.644399999996, "isoelectric_point": 9.81829319000244 }, "id": "APKECPOBEN_1041", "locus": "GCA002090215_01037" }, { "type": "cds", "sequence": "contig_1", "start": 585946, "stop": 586527, "strand": "-", "frame": 1, "gene": "yihA", "product": "ribosome biogenesis GTP-binding protein YihA/YsxC", "db_xrefs": [ "COG:COG0218", "COG:D", "GO:0000917", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0005829", "GO:0042254", "GO:0043022", "GO:0046872", "RefSeq:WP_010874837.1", "SO:0001217", "UniParc:UPI000003226E", "UniRef:UniRef100_P75303", "UniRef:UniRef50_P47577", "UniRef:UniRef90_P75303" ], "nt": "ATGGAGGCACGTTTTTTAAAAAGTGCAAGTGACTTAGAGAGTTGTCCCCAAGACAGTGTTAAGGAAGTCTGTTTTATGGGGCGGAGTAATGTCGGCAAATCAAGCCTCATTAATGCCTTTTTTCAAAAAAAGCTAGCTAAAACTTCCGCGACGCCGGGACGTACACAACTGTTAAACTTCTTTGAATACAACCGTAAACGCTTTGTCGATTTACCAGGTTATGGCTTTGCTAAACTGAGTAAAGTGCAAAAGGAAGCAATTACTAATTTATTAACGCAGTTCCTCAATTTTCGCCAAAACTTAACTGGTGTGGTACTAGTGATTGACAGCGGTGTAGTGACCGTTCAGGACCAGGAAGTGGTCAAAACCATTCTCCAAACGGGGTTAAATTTTTTGGTGATAGCCAATAAGTTTGATAAGCTCAACCAAAGCGAGCGCTTTCATACCCAGAATAAACTGGCACACTTTTTAAAAGTCAATCCTAATAAGTGTTTATTTGTTAGTGCCAAAACCGGGTATAACCTCCAAGTAATGCACAAACAAATCTTTGAGTTATTTAAAGCAGATGGACAAGCAATTTAG", "aa": "MEARFLKSASDLESCPQDSVKEVCFMGRSNVGKSSLINAFFQKKLAKTSATPGRTQLLNFFEYNRKRFVDLPGYGFAKLSKVQKEAITNLLTQFLNFRQNLTGVVLVIDSGVVTVQDQEVVKTILQTGLNFLVIANKFDKLNQSERFHTQNKLAHFLKVNPNKCLFVSAKTGYNLQVMHKQIFELFKADGQAI", "aa_hexdigest": "abd56841e4c3a5c5f3ceb4780214e371", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000003226E", "ncbi_nrp_id": "WP_010874837.1", "uniref100_id": "UniRef100_P75303", "db_xrefs": [ "SO:0001217", "UniParc:UPI000003226E", "RefSeq:WP_010874837.1", "UniRef:UniRef100_P75303" ] }, "ips": { "uniref100_id": "UniRef100_P75303", "uniref90_id": "UniRef90_P75303" }, "psc": { "uniref90_id": "UniRef90_P75303", "gene": "yihA", "product": "ribosome biogenesis GTP-binding protein YihA/YsxC", "uniref50_id": "UniRef50_P47577", "cog_id": "COG0218", "cog_category": "D", "go_ids": [ "GO:0000917", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0005829", "GO:0042254", "GO:0043022", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_P47577", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47577" ], "product": "Probable GTP-binding protein EngB" }, "genes": [ "yihA" ], "id": "APKECPOBEN_1042", "locus": "GCA002090215_01038" }, { "type": "cds", "sequence": "contig_1", "start": 586535, "stop": 586756, "strand": "-", "frame": 3, "gene": "yneF", "product": "UPF0154 MG335-1-like protein", "db_xrefs": [ "COG:COG3763", "COG:S", "GO:0016020", "KEGG:K09976", "RefSeq:WP_014574997.1", "SO:0001217", "UniParc:UPI00001833B6", "UniRef:UniRef100_Q9EXD7", "UniRef:UniRef50_Q9EXD7", "UniRef:UniRef90_Q9EXD7" ], "nt": "ATGAATGATCTTGCGCTTGCCTTAGGTTTGGGAATTCCCCTTTCCCTGTTAGTCGGTGTCATTATTGGCTATTTTATTTCTATCAAAATCTTTAAAAAACAGATTCGTGATAACCCACCAATCACCGAAAACCAAATTAAGGCGATGTATGCCAAAATGGGTCGTAAGCTTTCTGAAACACAAGTCAAGGAAATTATGCGCTCCATTAAAAACCAGAAGTAA", "aa": "MNDLALALGLGIPLSLLVGVIIGYFISIKIFKKQIRDNPPITENQIKAMYAKMGRKLSETQVKEIMRSIKNQK", "aa_hexdigest": "6b8518efc9d5c23459413584c515b25c", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001833B6", "ncbi_nrp_id": "WP_014574997.1", "uniref100_id": "UniRef100_Q9EXD7", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001833B6", "RefSeq:WP_014574997.1", "UniRef:UniRef100_Q9EXD7" ] }, "ips": { "uniref100_id": "UniRef100_Q9EXD7", "uniref90_id": "UniRef90_Q9EXD7" }, "psc": { "uniref90_id": "UniRef90_Q9EXD7", "gene": "YneF", "product": "UPF0154 protein MG335.1 homolog", "uniref50_id": "UniRef50_Q9EXD7", "cog_id": "COG3763", "cog_category": "S", "kegg_orthology_id": "K09976", "go_ids": [ "GO:0016020" ] }, "pscc": { "uniref50_id": "UniRef50_Q9EXD7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9EXD7" ], "product": "UPF0154 protein MG335.1 homolog" }, "genes": [ "yneF" ], "id": "APKECPOBEN_1043", "locus": "GCA002090215_01039" }, { "type": "cds", "sequence": "contig_1", "start": 586815, "stop": 587444, "strand": "+", "frame": 3, "gene": "wcaA", "product": "Processive diacylglycerol beta-glycosyltransferase", "db_xrefs": [ "COG:COG0463", "COG:M", "EC:2.4.1.-", "GO:0005886", "GO:0006071", "GO:0046467", "GO:0046509", "KEGG:K19004", "SO:0001217", "UniRef:UniRef50_Q9ZB73", "UniRef:UniRef90_P75302" ], "nt": "ATGAACAAGCTAATTAGCATTCTGGTTCCTTGTTACCAATCCCAGCCCTTTCTCGATCGCTTTTTCAAATCACTTTTAAAGCAAGACTGGAACGGTGTTAAAGTAATCTTCTTTAATGATAACAAGCCAGATCCAACATACGAGATCCTAAAACAATTTCAACAAGCACATCCACAACTAGCGATTGAAGTACATTGTGGTGAGAAAAATGTCGGTGTAGGCGGTTCACGTGATCAACTAATAAATTATGTAGACACACCCTACTTTTACTTTGTTGATCCTGATGATGAATTTAGTGATCCCAACTGTTTTAAGGCAATAGTGGAAACAATTCAAGGAGAAAACTTTGATATTGCAGTGTTAAACAGTATTGTTTACCTCCAAATGTTGAAGAATGACTTTTTAATTAAACACATTCCGCTCAAAAACATCTTTCAGGGCAAGGTAAAACTCAATCCTGATAACACCGTAAATCATTTGCACTATATCCAGAATAACGACCAGTACATTTGGAACATTGTCATCAACACCGCCTTTTTCAAAGCATTGGATTTGCAGTTTGTTAACCGCTTTATTGAAGACATTGCGGTGTGGTTTCCGATTATGTTTAAAGCCCAAAAAGTGCTGTGA", "aa": "MNKLISILVPCYQSQPFLDRFFKSLLKQDWNGVKVIFFNDNKPDPTYEILKQFQQAHPQLAIEVHCGEKNVGVGGSRDQLINYVDTPYFYFVDPDDEFSDPNCFKAIVETIQGENFDIAVLNSIVYLQMLKNDFLIKHIPLKNIFQGKVKLNPDNTVNHLHYIQNNDQYIWNIVINTAFFKALDLQFVNRFIEDIAVWFPIMFKAQKVL", "aa_hexdigest": "116bcb9caa314d6839faa26b1fee38b6", "start_type": "ATG", "rbs_motif": null, "genes": [ "wcaA" ], "truncated": "3-prime", "pseudogene": { "start": 586815, "stop": 587747, "observations": { "mutation": [ 587442, 587619 ], "stop": [ 587442, 587619 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75302", "query_cov": 1.0, "subject_cov": 0.6129032258064516, "identity": 1.0, "score": 436.0, "evalue": 7.44e-153, "gene_start": 1, "gene_end": 209, "reference_start": 1, "reference_end": 209, "reference_sequence": "MNKLISILVPCYQSQPFLDRFFKSLLKQDWNGVKVIFFNDNKPDPTYEILKQFQQAHPQLAIEVHCGEKNVGVGGSRDQLINYVDTPYFYFVDPDDEFSDPNCFKAIVETIQGENFDIAVLNSIVYLQMLKNDFLIKHIPLKNIFQGKVKLNPDNTVNHLHYIQNNDQYIWNIVINTAFFKALDLQFVNRFIEDIAVWFPIMFKAQKVLWIDVNGVNYYLRPNSASTQKNSIKLLSFIEAYERLYFHLKKVGKLADFIDPNNKIESRFWRRQAFIWFSFINVSWMKAEFEQTKSVLQKLFDFMEANGIYDRVFTNKHHGIYLLWVNRLKHFKKLVQAQPHL" }, "paralog": false, "identity": 0.9935483870967742, "score": 631.0, "evalue": 3.48e-232, "description": "Internal stop codon at 587442, 587619. Nonsense mutation around 587442, 587619.", "psc": { "uniref90_id": "UniRef90_P75302", "gene": "WcaA", "product": "Processive diacylglycerol beta-glycosyltransferase", "ec_ids": [ "2.4.1.-" ], "uniref50_id": "UniRef50_Q9ZB73", "cog_id": "COG0463", "cog_category": "M", "kegg_orthology_id": "K19004", "go_ids": [ "GO:0005886", "GO:0046509", "GO:0006071", "GO:0046467" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75302", "EC:2.4.1.-", "UniRef:UniRef50_Q9ZB73", "COG:COG0463", "COG:M", "KEGG:K19004", "GO:0005886", "GO:0046509", "GO:0006071", "GO:0046467" ] }, "pscc": { "uniref50_id": "UniRef50_Q9ZB73", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9ZB73" ], "product": "Processive diacylglycerol beta-glycosyltransferase" } }, "id": "APKECPOBEN_1044", "locus": "GCA002090215_01040" }, { "type": "cds", "sequence": "contig_1", "start": 587472, "stop": 587621, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCGACCGAACAGTGCTTCCACCCAGAAAAACAGCATTAAATTACTCTCCTTTATTGAGGCTTACGAACGGTTGTATTTCCACTTAAAGAAGGTGGGTAAGCTAGCTGATTTTATTGACCCTAACAATAAGATTGAAAGTCGTTTTTGA", "aa": "MRPNSASTQKNSIKLLSFIEAYERLYFHLKKVGKLADFIDPNNKIESRF", "aa_hexdigest": "9997683b229596b41da09e2cef7b4904", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5759.5947, "isoelectric_point": 9.77445468902588 }, "id": "APKECPOBEN_1045", "locus": "GCA002090215_01041" }, { "type": "cds", "sequence": "contig_1", "start": 587667, "stop": 587786, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGCGGAATTTGAACAGACCAAAAGTGTGTTGCAAAAACTGTTTGACTTCATGGAAGCCAACGGCATCTACGACAGGGTATTTACTAATAAGCACCACGGTATTTACTTATTGTGA", "aa": "MKAEFEQTKSVLQKLFDFMEANGIYDRVFTNKHHGIYLL", "aa_hexdigest": "a6aa4d2c1b90fa3fa9ade9edace266a8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4663.333899999999, "isoelectric_point": 6.704352378845214 }, "id": "APKECPOBEN_1046", "locus": "GCA002090215_01042" }, { "type": "cds", "sequence": "contig_1", "start": 587851, "stop": 588147, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_017532763.1", "SO:0001217", "UniParc:UPI0002F4AA32", "UniRef:UniRef100_UPI0002F4AA32", "UniRef:UniRef50_A0A0H3DKQ0", "UniRef:UniRef90_A0A0H3DKQ0" ], "nt": "TTGGCAACCGAGTTGCAAGTGGTAGCTATTTTTTCAATCTCCAAAAAACAAATATTAATTATGAAAAATAAAAAAATTATCAAACTACCTTTTATTGATCACGCAATGAATTCACTCAATCAAAGTGAGCTAATTTCATTTGTAGCTTTTAACGAAGCCAATGAAGATAATAAACGCACACTTTACATAAAGATTCACGCTAATCAAAAGGAAATGGTTGTACAAGCCCAAGATGGAAAACAAACTCTAGAAAGAGAACAACTTTTTAAAAAGCTACTCGAAGCTTTAGCTTGTTAG", "aa": "MATELQVVAIFSISKKQILIMKNKKIIKLPFIDHAMNSLNQSELISFVAFNEANEDNKRTLYIKIHANQKEMVVQAQDGKQTLEREQLFKKLLEALAC", "aa_hexdigest": "9836dd356fafd039539fa67574de5c51", "start_type": "TTG", "rbs_motif": "AAAA", "ups": { "uniparc_id": "UPI0002F4AA32", "ncbi_nrp_id": "WP_017532763.1", "uniref100_id": "UniRef100_UPI0002F4AA32", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002F4AA32", "RefSeq:WP_017532763.1", "UniRef:UniRef100_UPI0002F4AA32" ] }, "ips": { "uniref100_id": "UniRef100_UPI0002F4AA32", "uniref90_id": "UniRef90_A0A0H3DKQ0" }, "psc": { "uniref90_id": "UniRef90_A0A0H3DKQ0", "uniref50_id": "UniRef50_A0A0H3DKQ0" }, "pscc": { "uniref50_id": "UniRef50_A0A0H3DKQ0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0H3DKQ0" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11276.158399999993, "isoelectric_point": 9.098954963684083 }, "id": "APKECPOBEN_1047", "locus": "GCA002090215_01043" }, { "type": "cds", "sequence": "contig_1", "start": 588345, "stop": 588656, "strand": "-", "frame": 2, "gene": null, "product": "UPF0134 protein MPN_484", "db_xrefs": [ "RefSeq:WP_010874840.1", "SO:0001217", "UniParc:UPI000013AB4F", "UniRef:UniRef100_P75301", "UniRef:UniRef50_P75076", "UniRef:UniRef90_P75301" ], "nt": "ATGGGCTTTTATGGCAATTTAAACCATATGGAAAAACGAAAAAGTGGTTACGTTACCCAAAAGCAGTTTAATGATTTCAAAAATTCCAACAATCAGCGGCTGATAAAAATTGAGAACACTTTGGTTTCTCAAGGTGAACAAATTAGTCAGCTAATCAAAGTTTCAATTCTTCAGGGTGAACAAATAAATAAGCTCACTGAAACTGTTGAAAAGCAGGGCGAACAAATTCAAACCCAAGGGGAAACGCTTAAGTTAATTCTTGAAACTCTTCAAGTAATTAACAAACGTTTGGATCGTTTGGAGTCTAAGTAG", "aa": "MGFYGNLNHMEKRKSGYVTQKQFNDFKNSNNQRLIKIENTLVSQGEQISQLIKVSILQGEQINKLTETVEKQGEQIQTQGETLKLILETLQVINKRLDRLESK", "aa_hexdigest": "9af49134a83744b70dcf9c7cd697c5d8", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013AB4F", "ncbi_nrp_id": "WP_010874840.1", "uniref100_id": "UniRef100_P75301", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013AB4F", "RefSeq:WP_010874840.1", "UniRef:UniRef100_P75301" ] }, "ips": { "uniref100_id": "UniRef100_P75301", "uniref90_id": "UniRef90_P75301" }, "psc": { "uniref90_id": "UniRef90_P75301", "product": "UPF0134 protein MPN_484", "uniref50_id": "UniRef50_P75076" }, "pscc": { "uniref50_id": "UniRef50_P75076", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75076" ], "product": "UPF0134 protein MPN_038" }, "genes": [], "id": "APKECPOBEN_1048", "locus": "GCA002090215_01044" }, { "type": "cds", "sequence": "contig_1", "start": 589073, "stop": 589372, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATGGGTTCGGGTCAGGGGACCCAAGTTAAGGGCAGTGTGCAGGGGAGTAGCCGAGCAGCTTCTGTTAGCGTTCAAACTACCCAGCAAAGCAGACAGCAATCGACTGATACCCAAGAATCGGAAGTGGTTAAGTTAGCCAAAGCGCTGTTGAAATCAAGTGCGGATTTGGCCAAACCCTTCACTGATAACCCCACCTTTAAAAAGGCCTTAACCGACATCCAATCCGAGTACAAGGATTATTTGGCTGCTGCGGGGAAGTTAAGTGAGTTTAAGAAAGATTTGGGGGAGGTGAGTTAA", "aa": "MMGSGQGTQVKGSVQGSSRAASVSVQTTQQSRQQSTDTQESEVVKLAKALLKSSADLAKPFTDNPTFKKALTDIQSEYKDYLAAAGKLSEFKKDLGEVS", "aa_hexdigest": "5d20c19b69167cb033b3f3f4522231ad", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10546.648099999991, "isoelectric_point": 8.925663948059086 }, "id": "APKECPOBEN_1049", "locus": "GCA002090215_01045" }, { "type": "cds", "sequence": "contig_1", "start": 589418, "stop": 590185, "strand": "-", "frame": 3, "gene": null, "product": "DUF3713 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000A8A20B3", "UniRef:UniRef90_UPI000A8A20B3" ], "nt": "GTGACCAAGTCCAACCTGCGCGGGGCCTTTAAGGGCGTGGGGTTAGCTACGGCCATTATTGACCAGTACGAGTACCTGGTGGGTGGGAGTAAGACGAGTTCGTTGCCCGAGGTCAAGGTAGATTCGAATAAGTCCAACCAAAATCCGCTGGATAGTTTTTTTATGGAGGGGAAGGATGCGGTGGCGATTAGATCCATAGTTTCGCGTGCGAAAATTGCCATGACCGACCAAACCCCCGGGTTTAAGGTCGACCCGGCGTTTGTCAAGGTGAAGCAGAATAATCAAACCGATGCGTTTTACACAACCCAGCGCAAACTGAGCGGCGGGCAAACTAATGGGGGTAGTAATAATTCCAATGATAAACACCACTACCTCCAAGACGCGGTGCGGTTGACGAGTAGTCAAGCAATGGCGGCAGCTTCCACTGGGGCAGGTTCTAGTAGTGGTACCAATGTTGGTGGTAGCTCCGGGGGCAACTCTGTTTTAATTCCGCTCCCAAGGAGTGCCGCCCTTACCCACACCCAGCAACAAGTTCAACAAACCACCTCCACACTCCAAACCCCTGTTTACGCCCGTGGTGACGATGGTACATACGCCCTGGCAATTGACGGTGGTGATTATTTTTTGGCGAACAACAAACGCGATTTCACCAAACAAGCCGACATCTTGCTGTACCGCTATTTACAAGCTAAGAGTAATAACTTTAAGGAAAACGGCGTCGAGTTTAGTTTGAACTTACTGGAATCTGGCAGCCTGTTCCAAACGTGA", "aa": "MTKSNLRGAFKGVGLATAIIDQYEYLVGGSKTSSLPEVKVDSNKSNQNPLDSFFMEGKDAVAIRSIVSRAKIAMTDQTPGFKVDPAFVKVKQNNQTDAFYTTQRKLSGGQTNGGSNNSNDKHHYLQDAVRLTSSQAMAAASTGAGSSSGTNVGGSSGGNSVLIPLPRSAALTHTQQQVQQTTSTLQTPVYARGDDGTYALAIDGGDYFLANNKRDFTKQADILLYRYLQAKSNNFKENGVEFSLNLLESGSLFQT", "aa_hexdigest": "2b4236b3fa015140e42e2e005ce5e182", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 589115, "stop": 590299, "observations": { "mutation": [ 589420, 590218 ], "stop": [ 589420, 590218 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000A8A20B3", "query_cov": 1.0, "subject_cov": 0.625, "identity": 0.965, "score": 403.0, "evalue": 4.03e-138, "gene_start": 1, "gene_end": 255, "reference_start": 39, "reference_end": 293, "reference_sequence": "MFPEQKNGQTQQSNTSSRTGLFDWQTKWNSNGAANKLLVTKSNLRGAFKGVGLATAIIDQYEYLVGGSKTSSLPEVKVDSNKSNQNPLDSFFMEGKDAVAIRSIVSRAKIAMTDQTPGFKVDPAFVKVKQNNQTDAFYTTQRKLSGGQTNGGSNNSNDKHHYLQDAVRLTSSQAMAAASTGADSSSGTNVGGSSGGNSVLIPLPRSAALTHTQQQVQQTTSTLQTPVYARGDDGTYALAIDGGDYFLANNKRDFTKQADILLYRYLQAKSNNFKENGVEFSLNLLESGSLFQTWAQTGLTAKLYGALVAMMGSGQGTQVKGSVQGSSRAASVSVQTTQQSRQQSTDTQESEVVKLAKALLKSSADLAKPFTDNPTFKKALTDIQSEYKDYLAAAGKLSEFKKDLGEVS" }, "paralog": false, "identity": 0.9670050761421319, "score": 619.0, "evalue": 1.29e-225, "description": "Internal stop codon at 589420, 590218. Nonsense mutation around 589420, 590218.", "psc": { "uniref90_id": "UniRef90_UPI000A8A20B3", "product": "DUF3713 domain-containing protein", "uniref50_id": "UniRef50_UPI000A8A20B3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000A8A20B3", "UniRef:UniRef50_UPI000A8A20B3" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000A8A20B3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000A8A20B3" ], "product": "DUF3713 domain-containing protein" } }, "id": "APKECPOBEN_1050", "locus": "GCA002090215_01046" }, { "type": "cds", "sequence": "contig_1", "start": 590216, "stop": 590518, "strand": "-", "frame": 3, "gene": null, "product": "DUF3713 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0006B4FEFE", "UniRef:UniRef90_UPI0006B4FEFE" ], "nt": "TTGCTCTATCTGTTTCCCCTCACCCAAGCGTTCTTCGCGTACCAAGCACCCAAGGACGGGCTCGACAGCTTATACGACCAAGCCGCGATTGGTAGTGCCTTACAGTTGGGCTATGCCTTCCCCGCGTTTCGCGAGCCCAATAATGGACAAAGCCAAGGTAAAACCACCTTCGACCCCACCCCCAACAGTGCCCAAAACTTCGGGGATTTCATTAAGGCGGTGTTTCCGAAGCAGAAGAATGGTCAAACCCAACAATCCAATACATCCTCCCGCACCGGGTTGTTTGACTGGCAAACCAAGTGA", "aa": "MLYLFPLTQAFFAYQAPKDGLDSLYDQAAIGSALQLGYAFPAFREPNNGQSQGKTTFDPTPNSAQNFGDFIKAVFPKQKNGQTQQSNTSSRTGLFDWQTK", "aa_hexdigest": "8b792a088315fbfca1588b7abc8d103e", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 589987, "stop": 590518, "observations": { "insertion": [ 590029 ], "mutation": [ 590218 ], "stop": [ 590218 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0006B4FEFE", "query_cov": 1.0, "subject_cov": 0.5681818181818182, "identity": 0.98, "score": 200.0, "evalue": 3.22e-64, "gene_start": 1, "gene_end": 100, "reference_start": 1, "reference_end": 100, "reference_sequence": "MLYLFPLTQAFFAYQAPKDGLDSLYDQAAIGSALQLVYAFPAFREPNNGQSQGKTTFDPTPNSAQNFGDFIKAVFPEQKNGQTQQSNTSSRTGLFDWQTKWNSNGAANKLLVTKSNLRGAFKGVGLATAIIDQYEYLVGGSKTSSLPEVKVDSNKSNQNPLDSFLWRGRMPWRLDP" }, "paralog": false, "identity": 0.9715909090909091, "score": 342.0, "evalue": 2.81e-122, "description": "Internal stop codon at 590218. Frameshift due to insertion around 590029. Nonsense mutation around 590218.", "psc": { "uniref90_id": "UniRef90_UPI0006B4FEFE", "product": "DUF3713 domain-containing protein", "uniref50_id": "UniRef50_UPI0006B4FEFE", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0006B4FEFE", "UniRef:UniRef50_UPI0006B4FEFE" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0006B4FEFE", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0006B4FEFE" ], "product": "DUF3713 domain-containing protein" } }, "id": "APKECPOBEN_1051", "locus": "GCA002090215_01047" }, { "type": "cds", "sequence": "contig_1", "start": 590612, "stop": 590995, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00266.25" ], "nt": "ATGACCAAAACTAAGTTTAATCCGTACCAATTTCGCAAACAATTTAAGTGGTTTAAAAACAACCCCCAATGGGTTAACTTTGACAACGCTGCTACCTCAATTGCGCTAGACACCGTTAGTCAAGCTTGCAAGGAATACTATGAGCTTTTTAGTGTCAACCCCCACAATAAAACCCCTGATCTCAACAACCAGATTATTGCCATTATTGCTGAAACCCGTCAACTAGTAGCGGACTGGTTTAATGTCACTGCACTTGAAATTATCTTTACAAGTTCAGCCACGGAGTCCATTAATTTGTTTGCCCATGGCTTAAAGCCCTGGATTAAACCCGGTGATGAAATTGTCTTAAAGGGTGATGAACACTCGGCTAACGTTTTGCCCTGA", "aa": "MTKTKFNPYQFRKQFKWFKNNPQWVNFDNAATSIALDTVSQACKEYYELFSVNPHNKTPDLNNQIIAIIAETRQLVADWFNVTALEIIFTSSATESINLFAHGLKPWIKPGDEIVLKGDEHSANVLP", "aa_hexdigest": "dbc39caa0835e675c5a40bdf377bb78e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00266.25", "name": "Aminotran_5", "length": 104, "aa_cov": 0.8110236220472441, "hmm_cov": 1.0, "evalue": 3.3259410275176664e-31, "score": 103.9818344116211, "start": 25, "stop": 127 } ], "seq_stats": { "molecular_weight": 14545.323499999986, "isoelectric_point": 6.269079399108887 }, "id": "APKECPOBEN_1052", "locus": "GCA002090215_01048" }, { "type": "cds", "sequence": "contig_1", "start": 591080, "stop": 591838, "strand": "+", "frame": 2, "gene": "csd", "product": "putative cysteine desulfurase", "db_xrefs": [ "COG:COG0520", "COG:E", "EC:2.8.1.7", "GO:0031071", "SO:0001217", "UniRef:UniRef50_Q49420", "UniRef:UniRef90_P75298" ], "nt": "TTGATTAACCCGAAAACTAAAGTAGTAGCCATTACCGCTACTTCCAATTTGTTTGGCAATTCAATTGACTTTGCTCAAATTGCCGATTACCTCAAACAAGTCAATCCTAAAGCTTTTGTAGCCGTGGATGCAGTGCAAACGGTGCAGCACAAGCAAATTGACATTGCCAAAACCCAGATCGACTTCCTAGCCTTTTCTACTCATAAGTTTTACGGTCCCACTGGACTGGGGGTAGCTTACATTAAAAAAAGCTTACAACCCCAATTACAACCCCTCAAACTCGGGGGTGATATCTTTACCCAGATTGACCCGGACAATACAATTCACTTTAAAAGTACACCCCTAAAGTTTGAAGCCGGTACCCCCAACATTATGGCCATCTATGCCTTAAACAAGCTGTTGCGCTTCTTTAAACAAAAGTTTAATTTTGCCCAAATGATGGCTTATGGTCACCAACTTAAACAACAAGCTTACGAACTGTTAAACAGTAATCCGCAAATTGTGCTGGCCAACCACGACCAGGATGTCCCGATTTTTTCCTTTAAACACCGCCAATTGGCTACCATTGATCTAGCCACTTTCCTAAACATCAATAAGATCATGGTACGCCAGGGCTCCATTTGTGTGGGACGTTACCGCAATAAGGATTACTTTGTGAGAGTTTCGTTAATGCACTATAACACAGTGAAGGAGTTGCAGTATTTAGCTAAACTCCTAGCAACTGATACCAAGACAATTATTAAAAACGTAATTAAGTAA", "aa": "MINPKTKVVAITATSNLFGNSIDFAQIADYLKQVNPKAFVAVDAVQTVQHKQIDIAKTQIDFLAFSTHKFYGPTGLGVAYIKKSLQPQLQPLKLGGDIFTQIDPDNTIHFKSTPLKFEAGTPNIMAIYALNKLLRFFKQKFNFAQMMAYGHQLKQQAYELLNSNPQIVLANHDQDVPIFSFKHRQLATIDLATFLNINKIMVRQGSICVGRYRNKDYFVRVSLMHYNTVKELQYLAKLLATDTKTIIKNVIK", "aa_hexdigest": "909b1b1d7d90a41dc23da77e1572a388", "start_type": "TTG", "rbs_motif": null, "genes": [ "csd" ], "truncated": "5-prime", "pseudogene": { "start": 590780, "stop": 591838, "observations": { "mutation": [ 590993 ], "stop": [ 590993 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75298", "query_cov": 1.0, "subject_cov": 0.6176470588235294, "identity": 0.996, "score": 498.0, "evalue": 1e-175, "gene_start": 1, "gene_end": 252, "reference_start": 157, "reference_end": 408, "reference_sequence": "MTKTKFNPYQFRKQFKWFKNNPQWVNFDNAATSIALDTVSQACKEYYELFSVNPHNKTPDLNNQIIAIIAETRQLVADWFNVTALEIIFTSSATESINLFAHGLKPWIKPGDEIVLKGDEHSANVLPWVALAKQTKARLVWVEKQANQSLEDTFKSLINPKTKVVAITATSNLFGNSIDFAQIADYLKQVNPKAFVAVDAVQTVQHKQIDIAKTQIDFLAFSTHKFYGPTGLGVAYIKKSLQPQLQPLKLGGDIFTQIDPDNTIHFKSTPLKFEAGTPNIMAIYALNKLLRFFKQKFNFAQMMAYGHQLKQQAYELLNSNPQIVLANHDQDVPIFSFKHRQLATIDLATFLNINKIMVRQGSICVGRYRNKDYFVRVSLMHYNTVKELQYLAKLLATDTKTIIKNVIK" }, "paralog": false, "identity": 0.9971590909090909, "score": 692.0, "evalue": 1.39e-254, "description": "Internal stop codon at 590993. Nonsense mutation around 590993.", "psc": { "uniref90_id": "UniRef90_P75298", "gene": "csd", "product": "Probable cysteine desulfurase", "ec_ids": [ "2.8.1.7" ], "uniref50_id": "UniRef50_Q49420", "cog_id": "COG0520", "cog_category": "E", "go_ids": [ "GO:0031071" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75298", "EC:2.8.1.7", "UniRef:UniRef50_Q49420", "COG:COG0520", "COG:E", "GO:0031071" ] }, "pscc": { "uniref50_id": "UniRef50_Q49420", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49420" ], "product": "Probable cysteine desulfurase" } }, "id": "APKECPOBEN_1053", "locus": "GCA002090215_01049" }, { "type": "cds", "sequence": "contig_1", "start": 591841, "stop": 592263, "strand": "+", "frame": 1, "gene": "iscU", "product": "putative MG337-like protein", "db_xrefs": [ "COG:COG0822", "COG:O", "GO:0005506", "GO:0016226", "GO:0051536", "RefSeq:WP_010874844.1", "SO:0001217", "UniParc:UPI0000139648", "UniRef:UniRef100_P75297", "UniRef:UniRef50_P75297", "UniRef:UniRef90_P75297" ], "nt": "ATGGATCGCAAAATAAGGGAGCAAATTATTGCCATTTACAGCAACTTACAACACAAACAGGACTTAACTCAGTTTGCCCATTGTTTAACCACTAAGGCTGATGATAACTGTGAGGACTTCTTTAACTTAGGCTTACAGTTTCAAAACAACCAACTAACAGCACTTGGTTTTAACGGGGAGGGTTGTATTATTTCGACAATTGCTAGTGAACTGACTTTAAGTGCGTTGGAAGGCAAAACAATCAAAGAAGCCATTGTCTTGTTAGAACAGTTTATGGAAGCTGTACAAACTGGTAGCTTAAGCACGCCACTACCCCAAGCTTTACAACTGTTGTGGGACTTTCAGATTGACCAGAAGCGTTTAAACTGCTTACTCCTAACACCACAGAATTTGCTACAGTGGCTTAAAGATTTTTCGCACTAA", "aa": "MDRKIREQIIAIYSNLQHKQDLTQFAHCLTTKADDNCEDFFNLGLQFQNNQLTALGFNGEGCIISTIASELTLSALEGKTIKEAIVLLEQFMEAVQTGSLSTPLPQALQLLWDFQIDQKRLNCLLLTPQNLLQWLKDFSH", "aa_hexdigest": "3a5136abf5d565a383c1df3dff163993", "start_type": "ATG", "rbs_motif": "AAAA", "ups": { "uniparc_id": "UPI0000139648", "ncbi_nrp_id": "WP_010874844.1", "uniref100_id": "UniRef100_P75297", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139648", "RefSeq:WP_010874844.1", "UniRef:UniRef100_P75297" ] }, "ips": { "uniref100_id": "UniRef100_P75297", "uniref90_id": "UniRef90_P75297" }, "psc": { "uniref90_id": "UniRef90_P75297", "gene": "IscU", "product": "Uncharacterized protein MG337 homolog", "uniref50_id": "UniRef50_P75297", "cog_id": "COG0822", "cog_category": "O", "go_ids": [ "GO:0005506", "GO:0051536", "GO:0016226" ] }, "pscc": { "uniref50_id": "UniRef50_P75297", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75297" ], "product": "Uncharacterized protein MG337 homolog" }, "genes": [ "iscU" ], "id": "APKECPOBEN_1054", "locus": "GCA002090215_01050" }, { "type": "cds", "sequence": "contig_1", "start": 592244, "stop": 592420, "strand": "-", "frame": 3, "gene": null, "product": "DUF3713 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0005B22982", "UniRef:UniRef90_UPI0005B22982" ], "nt": "ATGGCTTTACTAGTAGAACAAGCCTTGGACGCTGGTACGCAAGAGTTAGCCTTTTTAGACTTAGTGCGCGCTAACCAGGTGCAAAACAAACGTAGTCACCAGCAAATCCTGGTGGGTGACAAACGCTTGTATGATGCGTTAGGGCAAACGCTAGCGCTTAGTGCGAAAAATCTTTAA", "aa": "MALLVEQALDAGTQELAFLDLVRANQVQNKRSHQQILVGDKRLYDALGQTLALSAKNL", "aa_hexdigest": "d3438a7ea1725d724407af1ebccd8493", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 592244, "stop": 592522, "observations": { "mutation": [ 592468 ], "stop": [ 592468 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0005B22982", "query_cov": 1.0, "subject_cov": 0.6304347826086957, "identity": 1.0, "score": 109.0, "evalue": 3.72e-30, "gene_start": 1, "gene_end": 58, "reference_start": 35, "reference_end": 92, "reference_sequence": "MSYVIQITNEDVNKISASWKTDKNSALGLKKEIFMALLVEQALDAGTQELAFLDLVRANQVQNKRSHQQILVGDKRLYDALGQTLALSAKNL" }, "paralog": false, "identity": 0.9782608695652174, "score": 171.0, "evalue": 1.02e-56, "description": "Internal stop codon at 592468. Nonsense mutation around 592468.", "psc": { "uniref90_id": "UniRef90_UPI0005B22982", "product": "DUF3713 domain-containing protein", "uniref50_id": "UniRef50_UPI0005B22982", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0005B22982", "UniRef:UniRef50_UPI0005B22982" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0005B22982", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0005B22982" ], "product": "DUF3713 domain-containing protein" } }, "id": "APKECPOBEN_1055", "locus": "GCA002090215_01051" }, { "type": "cds", "sequence": "contig_1", "start": 592466, "stop": 592954, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTCGACTGCTAGTGCCGGACTCACGGAACAAATCTTTAACAACTTTGGTCAGTTAGTAACAAGTAGTGACAAATCCGGGGCCTTGAGCCAGTACAAGGATAAAGCAACCCTGAAACGCTTAATCCAAAACACTAATTCCGATGCCGAGCTCAATGCCTTTGGTGAAGTGTTGCACCGTGCAGTTAATGTCGATACCAGTAACTTAGGACGTTTTAACTCCAGTGGGGAACCGTTGATTAGCTTTGACAACAAGAAGAAGTTCTTGGTTGATGTAGTGGACAAACTGGATGATGTTTACTTTAACAAGTTCGAAGGTTATGTTGGCCAAACCAAGGTGAAGATGTCTGATTCAAGTAGTAGTTCCCAAGGTAACGAAGACTATAAGAAAGCCAAAGCCGTACCACTCACCAAGGACTCGAGTGTCAAGACTTTGAGCTATGTCATTCAGATTACCAACGAGGACGTTAACAAAATTTCTGCTAGTTGA", "aa": "MSTASAGLTEQIFNNFGQLVTSSDKSGALSQYKDKATLKRLIQNTNSDAELNAFGEVLHRAVNVDTSNLGRFNSSGEPLISFDNKKKFLVDVVDKLDDVYFNKFEGYVGQTKVKMSDSSSSSQGNEDYKKAKAVPLTKDSSVKTLSYVIQITNEDVNKISAS", "aa_hexdigest": "1b738c5695efc357d89d0ea1556dc36c", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17721.500700000004, "isoelectric_point": 6.587207221984863 }, "id": "APKECPOBEN_1056", "locus": "GCA002090215_01052" }, { "type": "cds", "sequence": "contig_1", "start": 593135, "stop": 593290, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAGCTATGATGATACGGCTACGGCATCAGCAGCCGCAGCAAAAGCGCAAGTAGCAGTATTAAAGACAGCTCAGGCAACAAATACTCAAAGCGACAACCCCTTCAATAAGTTTGTCCAAAACCCCGATTATGTCCAGGGTTCGGAAACCAACTGA", "aa": "MSYDDTATASAAAAKAQVAVLKTAQATNTQSDNPFNKFVQNPDYVQGSETN", "aa_hexdigest": "345d1816f56bb0a4f4457f4db144b964", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5381.7197000000015, "isoelectric_point": 4.359459877014161 }, "id": "APKECPOBEN_1057", "locus": "GCA002090215_01053" }, { "type": "cds", "sequence": "contig_1", "start": 593375, "stop": 593551, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTTCACTTTCAAGTAGCGCAGCAGTTGCAAATGCTGTAGCTTCAAGTGGCATGCAAAAATCTGGTGATCAAACCCTCTTGGAACTCGGTAAAAAAGCGTTTGAAAGTGAACTGGAAGCATCTAGTAGTGATGGGCAGTACAAGTACCTGCGCTTCTTGTCCACCTTAATGTGA", "aa": "MVSLSSSAAVANAVASSGMQKSGDQTLLELGKKAFESELEASSSDGQYKYLRFLSTLM", "aa_hexdigest": "b379422fe7e084317980c5e62fef166e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6121.832899999998, "isoelectric_point": 5.052213478088378 }, "id": "APKECPOBEN_1058", "locus": "GCA002090215_01054" }, { "type": "cds", "sequence": "contig_1", "start": 593897, "stop": 594547, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATGGGTTCGGGTCAGGGGACCCAAGTTAAGGGCAGTGTGCAGGGGAGTAGCCGAGCAGCTTCTGTTAGCGTTCAAACTACCCAGCAAAACAGACAGCAATCGACTGATACCCAAGAATCGGAAGTGGTTAAGTTAGCCAAGTCCCTGCTGAAATCAAGTGCGGATTTGGCCAAACCCTTCACTGATAACCCCACCTTTAAAAAGGCCTTAACCGACATCCAATCCGAGTACAAGGATTATTTGGCTGCTGCGGGGAAGTTAAGTGAGTTTAAGAAAGATTTGGGGGAGGTGAGTGGCTTACAGCAAGCTATTATTGACCGCGCTGATAAGTACATTCAACTAGAAAAACAAGCCCAAAAGAGTGCGATTGGCTTGGGCCAACCGCTCCCTTACCAACGCGCTAGTGATGGTAGTTACCCCGCTTTAGAGAAGTTCTTTATTCCGGAAGATAGTGCAGCAGATGGAAAAGTAAAAGCATCTGAATCAGGATCTGCCGCTTTAGTAACACTAAAGACAACAGATAGTCAAAAAAGCACAAACACCGTTAAACAACCGGACATCAAACCGACCCGGGAAAACAACGACAAAAAGTTAAAGCAGTTAACTAGTGATGTAGAAACTAAAGCATCTAGTTTAATCACCAAATGA", "aa": "MMGSGQGTQVKGSVQGSSRAASVSVQTTQQNRQQSTDTQESEVVKLAKSLLKSSADLAKPFTDNPTFKKALTDIQSEYKDYLAAAGKLSEFKKDLGEVSGLQQAIIDRADKYIQLEKQAQKSAIGLGQPLPYQRASDGSYPALEKFFIPEDSAADGKVKASESGSAALVTLKTTDSQKSTNTVKQPDIKPTRENNDKKLKQLTSDVETKASSLITK", "aa_hexdigest": "b23149395fbb73e67e1f2ca081500eeb", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 23251.751700000004, "isoelectric_point": 9.17064380645752 }, "id": "APKECPOBEN_1059", "locus": "GCA002090215_01055" }, { "type": "cds", "sequence": "contig_1", "start": 594593, "stop": 595354, "strand": "-", "frame": 3, "gene": null, "product": "putative protein MPN_485", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75300", "UniRef:UniRef90_P75300" ], "nt": "GTGACCAAGTCCAACCTGCGCGGGGCCTTTAAGGGCGTGGGGTTAGCTACGGCCATTATTGACCAGTACGAGTACCTGGTGGGTGGAAAAAAAACGAGTTCGTTGCCCGAGGTCAAGGTAGATTCGAATAAGTCCAACCAAAATCCGCTGGATAGTTTTTTTATGGAGGGGAAGGATGCGGTGGCGATTAGATCCATAGTTTCGCGTGCGAAAATTGCCATGACCGACCAAACCCCCGGGTTTAAGGTCAACCCGGCGTTTGTCAAGGTGAAGCAGAGTCAGCAGAACGATACCTTTTACCAAAACCAGCGCAAACTGAGCGGCGGGCAAAGTGGGGATAATAACTCCCAGGGTAAACACCACTACCTCCAAGACGCGGTGCGGTTGACGAGTAGTCAAGCAATGGCGGCAGCTTCTACTGGGGCAGATTCTAGTAGTGGTACCAATGTTGGTGGTAGCTCCGGGGGCAACTCTGTTTTAATTCCGCTCCCAAGGAGTGCCGCCCTTACCCACACCCAGCAACAAGTTCAACAAACCACCTCCACACTCCAAACCCCTGTTTACGCCCGTGGTGACGATGGTACATACGCCCTGGCAATTGACGGTGGTGATTATTTTTTGGCGAACAACAAACGCGATTTCACCAAACAAGCCGACATCTTGCTGTACCGCTATTTACAAGCTAAGAGTAATAACTTTAAGGAAAACGGCGTCGAGTTTAGTTTGAACTTACTGGAATCTGGCAGCCTGTTCCAAACGTGA", "aa": "MTKSNLRGAFKGVGLATAIIDQYEYLVGGKKTSSLPEVKVDSNKSNQNPLDSFFMEGKDAVAIRSIVSRAKIAMTDQTPGFKVNPAFVKVKQSQQNDTFYQNQRKLSGGQSGDNNSQGKHHYLQDAVRLTSSQAMAAASTGADSSSGTNVGGSSGGNSVLIPLPRSAALTHTQQQVQQTTSTLQTPVYARGDDGTYALAIDGGDYFLANNKRDFTKQADILLYRYLQAKSNNFKENGVEFSLNLLESGSLFQT", "aa_hexdigest": "0d8906a50234f53318bc3abe30f76133", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 594290, "stop": 595654, "observations": { "mutation": [ 594595, 595387 ], "stop": [ 594595, 595387 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75300", "query_cov": 1.0, "subject_cov": 0.49317738791423, "identity": 0.992, "score": 478.0, "evalue": 4.41e-166, "gene_start": 1, "gene_end": 253, "reference_start": 102, "reference_end": 354, "reference_sequence": "FFAYQAPKDGLDSLYDQAAIGSALQLVYAFPAFREPNNGQSQGKTTFDPTPNSAQNFGDFIKAVFPEQKNGQTQQSNTSSRTGLFDWQTKWNSNGAANKLLVTKSNLRGAFKGVGLATAIIDQYEYLVGGSKTSSLPEVKVDSNKSNQNPLDSFFMEGKDAVAIRSIVSRAKIAMTDQTPGFKVNPAFVKVKQSQQNDTFYQNQRKLSGGQSGDNNSQGKHHYLQDAVRLTSSQAMAAASTGADSSSGTNVGGSSGGNSVLIPLPRSAALTHTQQQVQQTTSTLQTPVYARGDDGTYALAIDGGDYFLANNKRDFTKQADILLYRYLQAKSNNFKENGVEFSLNLLESGSLFQTWAQTGLTAKLYGALVAMMGSGQGTQVKGSVQGSSRAASVSVQTTQQSRQQSTDTQESEVVKLAKSLLKSSADLAKPFTDNPTFKKALTDIQSEYKDYLAAAEKLSEFKKDLGEVSGLQQAIIDRADKYIQLEKQAQKSAIGLGQPLPYQRASDXVVTPL" }, "paralog": false, "identity": 0.947136563876652, "score": 832.0, "evalue": 5.45e-308, "description": "Internal stop codon at 594595, 595387. Nonsense mutation around 594595, 595387.", "psc": { "uniref90_id": "UniRef90_P75300", "product": "Uncharacterized protein MPN_485", "uniref50_id": "UniRef50_P75300", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75300", "UniRef:UniRef50_P75300" ] }, "pscc": { "uniref50_id": "UniRef50_P75300", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75300" ], "product": "Uncharacterized protein MPN_485" } }, "id": "APKECPOBEN_1060", "locus": "GCA002090215_01056" }, { "type": "cds", "sequence": "contig_1", "start": 595385, "stop": 595690, "strand": "-", "frame": 3, "gene": null, "product": "DUF3713 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0006B4FEFE", "UniRef:UniRef90_UPI0006B4FEFE" ], "nt": "GTGGAGGAAAACCCCCTCCCCCTCACCCAAGCCTTCTTCGCGTACCAAGCACCCAAGGACGGGCTCGACAGCTTATACGACCAAGCCGCGATTGGTAGTGCCTTACAGTTGGGCTATGCCTTCCCCGCGTTTCGCGAGCCCAATAATGGACAAAGCCAAGGTAAAACCACCTTCGACCCCACCCCCAACAGTGCCCAAAACTTCGGGGATTTCATTAAGGCGGTGTTTCCGGAGCAGAAGAATGGTCAAACCCAACAATCCAATACATCCTCCCGCACCGGGTTGTTTGACTGGCAAACCAAGTGA", "aa": "MEENPLPLTQAFFAYQAPKDGLDSLYDQAAIGSALQLGYAFPAFREPNNGQSQGKTTFDPTPNSAQNFGDFIKAVFPEQKNGQTQQSNTSSRTGLFDWQTK", "aa_hexdigest": "ed2067ba64981a82eb1574b3db4a6a8e", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 595156, "stop": 595672, "observations": { "insertion": [ 595198 ], "mutation": [ 595387 ], "stop": [ 595387 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0006B4FEFE", "query_cov": 0.9405940594059405, "subject_cov": 0.5397727272727273, "identity": 0.9890000000000001, "score": 191.0, "evalue": 1.07e-60, "gene_start": 7, "gene_end": 101, "reference_start": 6, "reference_end": 100, "reference_sequence": "MLYLFPLTQAFFAYQAPKDGLDSLYDQAAIGSALQLVYAFPAFREPNNGQSQGKTTFDPTPNSAQNFGDFIKAVFPEQKNGQTQQSNTSSRTGLFDWQTKWNSNGAANKLLVTKSNLRGAFKGVGLATAIIDQYEYLVGGSKTSSLPEVKVDSNKSNQNPLDSFLWRGRMPWRLDP" }, "paralog": false, "identity": 0.9766081871345029, "score": 333.0, "evalue": 9.19e-119, "description": "Internal stop codon at 595387. Frameshift due to insertion around 595198. Nonsense mutation around 595387.", "psc": { "uniref90_id": "UniRef90_UPI0006B4FEFE", "product": "DUF3713 domain-containing protein", "uniref50_id": "UniRef50_UPI0006B4FEFE", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0006B4FEFE", "UniRef:UniRef50_UPI0006B4FEFE" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0006B4FEFE", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0006B4FEFE" ], "product": "DUF3713 domain-containing protein" } }, "id": "APKECPOBEN_1061", "locus": "GCA002090215_01057" }, { "type": "cds", "sequence": "contig_1", "start": 595694, "stop": 595846, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCAGTTGACTGATGGTAGTGATCAGTTTAAACCCACTACCAAAGATAACCTCGACAGTACTAGCAACAAAATCAAGTTTGTTAATTCCAAACCAAATGATCCTAACGGGGAGTTCTTTGCTAACCTCCAGGCTTATCTATTTGCCCAGTGA", "aa": "MQLTDGSDQFKPTTKDNLDSTSNKIKFVNSKPNDPNGEFFANLQAYLFAQ", "aa_hexdigest": "55cc7661bf6274e0c4f2776d45f597bc", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5627.124100000001, "isoelectric_point": 4.854868507385253 }, "id": "APKECPOBEN_1062", "locus": "GCA002090215_01058" }, { "type": "cds", "sequence": "contig_1", "start": 595973, "stop": 596281, "strand": "-", "frame": 3, "gene": null, "product": "DUF3713 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FE6F233", "UniRef:UniRef90_UPI002FE6F233" ], "nt": "GTGGTGCTAGCGGCGTGTTCTGCACTCAATACCTCCAATCTGTTTCCCCGTCAAAACCGCTCCAAGCAGTTGATTGGGTTTACCGAAAACAACATCATTAAACCTGAAGCTGTACTAAAAGCCGCTTTAGCTGAGGACAATGGTACGGAAACCATTTTAAGGGTTAACTTTGGTGAAGCGTTAAAGAGTTGGTACCAAAACAATAAGGATCGCAACATTGCTACCCGTTTAACTATCTTTAGTGAAAACGTGGAGGATGAACATGATAACCTGTTGGACCAAAAACAACAGGCCGAACCGATTAATTGA", "aa": "MVLAACSALNTSNLFPRQNRSKQLIGFTENNIIKPEAVLKAALAEDNGTETILRVNFGEALKSWYQNNKDRNIATRLTIFSENVEDEHDNLLDQKQQAEPIN", "aa_hexdigest": "807c034373d4ba9a80798388f61d708a", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 595670, "stop": 596278, "observations": { "mutation": [ 595696, 595921, 595975 ], "stop": [ 595696, 595921, 595975 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI002FE6F233", "query_cov": 0.9901960784313726, "subject_cov": 0.4469026548672566, "identity": 0.99, "score": 197.0, "evalue": 4.02e-62, "gene_start": 2, "gene_end": 102, "reference_start": 1, "reference_end": 101, "reference_sequence": "MLAACSALNTSNLFPRQNRSKQLIGFTENNIIKPEAVLKAALAEDNGTETILRVNFGEALKSWYQNNKDRNIATRLTIFSENVEDEHDNLLDQKQQAEPINWPIELQKEYDQWGGSESSWKALKLYDRLIADFQSLIFSNVVANVQLTDGSDQFKPTTKDNLDSTSNKIKFVNSKPNDPNGEFFANLQAYLFAQWVVEENPLPLTQAFFAYQAPKDGLDSLYDQAA" }, "paralog": false, "identity": 0.9752475247524752, "score": 390.0, "evalue": 8.39e-141, "description": "Internal stop codon at 595696, 595921, 595975. Nonsense mutation around 595696, 595921, 595975.", "psc": { "uniref90_id": "UniRef90_UPI002FE6F233", "product": "DUF3713 domain-containing protein", "uniref50_id": "UniRef50_UPI002FE6F233", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI002FE6F233", "UniRef:UniRef50_UPI002FE6F233" ] }, "pscc": { "uniref50_id": "UniRef50_UPI002FE6F233", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FE6F233" ], "product": "DUF3713 domain-containing protein" } }, "id": "APKECPOBEN_1063", "locus": "GCA002090215_01059" }, { "type": "cds", "sequence": "contig_1", "start": 596860, "stop": 597870, "strand": "-", "frame": 1, "gene": "recA", "product": "recombinase RecA", "db_xrefs": [ "COG:COG0468", "COG:L", "GO:0003677", "GO:0003684", "GO:0003697", "GO:0005524", "GO:0005829", "GO:0006281", "GO:0006310", "GO:0008094", "GO:0009432", "GO:0016887", "GO:0140664", "KEGG:K03553", "RefSeq:WP_010874846.1", "SO:0001217", "UniParc:UPI00001334FE", "UniRef:UniRef100_P78014", "UniRef:UniRef50_P78014", "UniRef:UniRef90_P78014" ], "nt": "ATGGTACAAAAAGAAATGATTAATAAGAAAATTAGCCAAGACAACAGTTTTATTCAAAACAATAACTTGGCAGACTTTGATTTTTTAGATGCAAAGAAAAACAGTGAAATTAAAACTGTCTCAACTGGAAGCCTTCATTTAGATGAAGCGCTCGGCACTGGTGGTTTGCCACTAGGGCGCATTGTAGAACTTTATGGTAACGAGTCCTCGGGTAAAACCACGGTCGCGTTACATGCAGTCGCTAGCTTTCAAAAAGCGGGCAAAGTGGCCTGTTACATTGATGCTGAGGGTGCACTAGATTTAAGTTACGCCAAGGCAATTGGGATTGACTTGGGTAAATTACTAGTAGCCCATCCCAAGCATGGGGAAAACGCCTTTGCCCTAATGGAGTCACTGATTAAGACCAACAAAGTGGCCTTAATTGTGGTGGACTCCGTGGCGGCTTTGATTCCTAAGCAAGAACTGGAGGGCAACATGGATGACCAGACAATAGGACTCCATGCGCGGATGATGTCTAAGGGACTACGCCGTGTGCAGTCACTCTTACCTGAATCTGACACCTGCTTACTGTTTATTAACCAACTCCGGGAAAAACCCGGAGTCATGTTTGGTAATGGGGAAGTAACTACTGGGGGTCGGGCGTTGAAGTTCTACGCTTCGATGCGGATGGAGGCCAAACGCAGTGAACTGCTCAAAGACCGCTTTGGTAACTATGTCGGCATTAAGAGTAAGTTAACCGTGTCCAAGAATAAGGTAGCGCGCCCGTTTGGTGTTGCCTTTTTGGAAATTATGTTTAACCGCGGTATTGTGTATGAACACGAAGTGATTGAGTTAGCGCTTAAACACAATGTGGTGGTGCGCAGTGACAATGCATATTCGTTTAAGAGCCAAAACATTGCGATTGGCAAAGAGAAGCTCTTTAGCGTGTTGGCGGAAAAACCAGAGTTGTTTGAACAAATTAAGCAGCTCACGATTAAGCAAATCCACAGCCCGCCACCCCCAGCGAGCTAG", "aa": "MVQKEMINKKISQDNSFIQNNNLADFDFLDAKKNSEIKTVSTGSLHLDEALGTGGLPLGRIVELYGNESSGKTTVALHAVASFQKAGKVACYIDAEGALDLSYAKAIGIDLGKLLVAHPKHGENAFALMESLIKTNKVALIVVDSVAALIPKQELEGNMDDQTIGLHARMMSKGLRRVQSLLPESDTCLLFINQLREKPGVMFGNGEVTTGGRALKFYASMRMEAKRSELLKDRFGNYVGIKSKLTVSKNKVARPFGVAFLEIMFNRGIVYEHEVIELALKHNVVVRSDNAYSFKSQNIAIGKEKLFSVLAEKPELFEQIKQLTIKQIHSPPPPAS", "aa_hexdigest": "d278d8e2ad4383582cac2f1e1bd6f327", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001334FE", "ncbi_nrp_id": "WP_010874846.1", "uniref100_id": "UniRef100_P78014", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001334FE", "RefSeq:WP_010874846.1", "UniRef:UniRef100_P78014" ] }, "ips": { "uniref100_id": "UniRef100_P78014", "uniref90_id": "UniRef90_P78014" }, "psc": { "uniref90_id": "UniRef90_P78014", "gene": "recA", "product": "recombinase RecA", "uniref50_id": "UniRef50_P78014", "cog_id": "COG0468", "cog_category": "L", "kegg_orthology_id": "K03553", "go_ids": [ "GO:0003677", "GO:0003684", "GO:0003697", "GO:0005524", "GO:0005829", "GO:0006281", "GO:0006310", "GO:0008094", "GO:0009432", "GO:0016887", "GO:0140664" ] }, "pscc": { "uniref50_id": "UniRef50_P78014", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P78014" ], "product": "Protein RecA" }, "genes": [ "recA" ], "id": "APKECPOBEN_1064", "locus": "GCA002090215_01060" }, { "type": "cds", "sequence": "contig_1", "start": 597944, "stop": 598417, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATTAACACTTTGGCTGGTGTTGGGCGCTGTCGGAGTCGGTGCAGTTGGTACGGGTGTGGGCTTTGGTACCAAGTACTTTTTAGACCACAAAGCCAAAAGTAACGTGGACGTTGCGTTTAAACAACGTTCACTACGCAAACAGGTCAATGTGGGCTTTTGGAATGCACTCAACTTCTTGGGTAGTGAAAGAAGTTTCGGTAACCAAAACAGTAAGGTTAAAACCAAGGGGATTGCTGAAATTATTAACCTCCTTAAGTACGACATTGTCGGTTTAGCGGAAATTCAACCATCCACTGAAAGTACTGCTGAACGGTTTGTCGACCAACTCAACGAGTACGCTGATCCTTATACCTCCTGGTCTTATGATCTATCTCCCGTAACTACCAGTAAGACTGCTAGTGAAGGCCAGAAGGAACGGATCTTAATTGTTTACAACAGCCAGAGTGTCAAAATGGAGGGACAAGGTTGA", "aa": "MKLTLWLVLGAVGVGAVGTGVGFGTKYFLDHKAKSNVDVAFKQRSLRKQVNVGFWNALNFLGSERSFGNQNSKVKTKGIAEIINLLKYDIVGLAEIQPSTESTAERFVDQLNEYADPYTSWSYDLSPVTTSKTASEGQKERILIVYNSQSVKMEGQG", "aa_hexdigest": "6250377bbbcdca629b3c59f75f708c0e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17288.397799999988, "isoelectric_point": 9.07142696380615 }, "id": "APKECPOBEN_1065", "locus": "GCA002090215_01061" }, { "type": "cds", "sequence": "contig_1", "start": 598436, "stop": 598834, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGACATTAGTAACTATGTCAGTGATGGGAGTTCTTCCAAAACAAGAACACCTAAGAAAACTAAAACTAGTAAAAAGAAGCCTATTAAAAAGAAAAGTTCAAAAAGCAAATCTTCCAAAGGTAGTAAGAAACAAAAAACTACCAATGAAAGTGAAAGTGAAACTTTAGAACTCAAACTGGAACAACGCCGTGTAACAACACGCTCACAATCAAAGCAACAAAAGGGGCAAGAACAAGCAACTGATCAAACTGATAGTGAGGGTGTGACTACTGAAGAAGGAGCAGATAACACTGACACTGAATTAGTTGAAACCACAGCGGAAACAACCGAACAAGAAGCTACCACGAAAAGTACAAAAGACACTAAGGAAACTAAAGATAGAACCAAAGTGGACTGA", "aa": "MDISNYVSDGSSSKTRTPKKTKTSKKKPIKKKSSKSKSSKGSKKQKTTNESESETLELKLEQRRVTTRSQSKQQKGQEQATDQTDSEGVTTEEGADNTDTELVETTAETTEQEATTKSTKDTKETKDRTKVD", "aa_hexdigest": "cf0b50ffedcbd7ad578aa351d0f85d5a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14670.803399999986, "isoelectric_point": 9.27933750152588 }, "id": "APKECPOBEN_1066", "locus": "GCA002090215_01062" }, { "type": "cds", "sequence": "contig_1", "start": 598886, "stop": 599143, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGCTTTTGGTCATTTTGACTCTCCGGGGGCAAACACTAAGCGGGGTGAAAAAATCGCTAAGATGGGCAAGGGTCAAGGTGCCCATGAATACTTTGAAGCGCAAACTACCTTCAAGGCAATGGATTCCATTAAGCAGCACTTTAACAACGAAAACATCCTGTTTATGGCTGACACTAACATTAAATTCGGTAATCAAGCCGCTGCTTTTGGTGAAAGCAAAGATTACACCTTTTTAACTGAAGATAATGAAGCGTGA", "aa": "MAFGHFDSPGANTKRGEKIAKMGKGQGAHEYFEAQTTFKAMDSIKQHFNNENILFMADTNIKFGNQAAAFGESKDYTFLTEDNEA", "aa_hexdigest": "d8169903d19ba3a30488b160954a6716", "start_type": "GTG", "rbs_motif": "AAAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9459.345299999994, "isoelectric_point": 5.492601966857909 }, "id": "APKECPOBEN_1067", "locus": "GCA002090215_01063" }, { "type": "cds", "sequence": "contig_1", "start": 599615, "stop": 600262, "strand": "-", "frame": 3, "gene": "ulaE", "product": "L-ribulose-5-phosphate 3-epimerase", "db_xrefs": [ "COG:COG3623", "COG:G", "EC:5.1.3.22", "GO:0005975", "GO:0016861", "GO:0019854", "GO:0034015", "SO:0001217", "UniRef:UniRef50_P75294", "UniRef:UniRef90_P75294" ], "nt": "TTGAGCATTGATGAATCGGATGCTAGGTTACAACGCTTGGATTGGAGTGATACCGAAATTAACCAACTCCACAATGAGTTACAAGCACAAACCTTTTGTTTAAACTCAATGTGTTTAAGTGCCCACCGGCGCTTTCCTTTGGGTTCCAAGAATAAAACTACTGTCCAACAGGGCTTAACCATTTTTGAAAAGGCCTGTGTGTTAGCACGCAAACTAGGGATTCGTATTATCCAGTTAGCGGCTTATGATGTGTATTATGAGCCACATGACACAGAAACAGAACGCAATTTCATTACTAACATGCGGAAAGTGGCAGAGTTAGCGCAAAAGTACGCTGTTACCATTGCCTTTGAGGTGATGGACACGCCGTTTGCTGGCACGATTGTGCGTTGTTTAAACTTGATTAAGCGGATTGGTAAGGCTAACATTTTGCTCTATCCGGATATTGGTAACCTATCGCAATTTAGTACGGCAGTCTTTGATGAAATCGCATTAGGTCAAGACAAGATAGTGGGCTTTCACTTTAAAGATACTTTACCCAAGCAGTTTAAGGAAGTACCGTTTGGCACAGGTACAGCCCAATTTGAAGCAGCTTTAAAAGCCATTCACCAGTATGTACCAACAGTACCAATTCTAATTGAAATGTGA", "aa": "MSIDESDARLQRLDWSDTEINQLHNELQAQTFCLNSMCLSAHRRFPLGSKNKTTVQQGLTIFEKACVLARKLGIRIIQLAAYDVYYEPHDTETERNFITNMRKVAELAQKYAVTIAFEVMDTPFAGTIVRCLNLIKRIGKANILLYPDIGNLSQFSTAVFDEIALGQDKIVGFHFKDTLPKQFKEVPFGTGTAQFEAALKAIHQYVPTVPILIEM", "aa_hexdigest": "5a012f6ffce7f5176551567a9fdeded2", "start_type": "TTG", "rbs_motif": null, "genes": [ "ulaE" ], "truncated": "both", "pseudogene": { "start": 599507, "stop": 600424, "observations": { "mutation": [ 599617, 600313 ], "stop": [ 599617, 600313 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75294", "query_cov": 1.0, "subject_cov": 0.7049180327868853, "identity": 0.995, "score": 427.0, "evalue": 4.19e-150, "gene_start": 1, "gene_end": 215, "reference_start": 55, "reference_end": 269, "reference_sequence": "MLVIHFKPYNNLKMSFTSTENKHLLGVYEKAINNKFAWKDKIAIAKQASFDFIELSIDESDARLQRLDWSDTEINQLHNELQAQTFCLNSMCLSAHRRFPLGSKNKTTVQQGLTIFEKACVLARKLGIRIIQLAAYDVYYEPHDTETERNFITNMRKVAELAQKYAVTIAFEVMDTPFAGTIVRCLNLIKRIGKANILLYPDIGNLSQFSTAVFDEIALGQDKIVGFHFKDTLPKQFKEVPFGTGTAQFEAALKAIHQYVPTVPILIEMWSKNDPAESTVQNVAQLKQAKQFYEQQWDLALKRVK" }, "paralog": false, "identity": 0.9934426229508196, "score": 599.0, "evalue": 3.54e-220, "description": "Internal stop codon at 599617, 600313. Nonsense mutation around 599617, 600313.", "psc": { "uniref90_id": "UniRef90_P75294", "gene": "ulaE", "product": "L-ribulose-5-phosphate 3-epimerase", "ec_ids": [ "5.1.3.22" ], "uniref50_id": "UniRef50_P75294", "cog_id": "COG3623", "cog_category": "G", "go_ids": [ "GO:0005975", "GO:0016861", "GO:0019854", "GO:0034015" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75294", "EC:5.1.3.22", "UniRef:UniRef50_P75294", "COG:COG3623", "COG:G", "GO:0005975", "GO:0016861", "GO:0019854", "GO:0034015" ] }, "pscc": { "uniref50_id": "UniRef50_P75294", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75294" ], "product": "Probable L-ribulose-5-phosphate 3-epimerase UlaE" } }, "id": "APKECPOBEN_1068", "locus": "GCA002090215_01064" }, { "type": "cds", "sequence": "contig_1", "start": 600366, "stop": 600545, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAAACGCTTAGCGGATTTGGGCTTTAAGGTCACCGTTACCGGCGGCATTACCCTCAACGACATCCAGTTATTTAAGGATATTCCCATTTATATCTTTATTGCTGGGCGTACCATTAGGGATGCTAGTGATCCACTTCAAACCGTACAACAATTTAAAGATGAGTTTCACAAGTACTGA", "aa": "MKRLADLGFKVTVTGGITLNDIQLFKDIPIYIFIAGRTIRDASDPLQTVQQFKDEFHKY", "aa_hexdigest": "cf70789c30d3148d68fda7796cf1e1f8", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6787.792700000002, "isoelectric_point": 8.085318565368652 }, "id": "APKECPOBEN_1069", "locus": "GCA002090215_01065" }, { "type": "cds", "sequence": "contig_1", "start": 600636, "stop": 601016, "strand": "-", "frame": 2, "gene": null, "product": "orotidine 5'-phosphate decarboxylase / HUMPS family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00387B8401" ], "nt": "ATGGCTTTACCTTTAATCCAAATTGCGCTTGATAACCTCAGTTTAGCGAGTGCTTTAAATGACCTGGCTAAAGTCGGGGATGCTGTTGATGTTATTGAAGTGGGCACTATCTTACTAACAGCGGAGGGCGTTAATGCTGTTAAAGAAATTGCCAAACGCTATCCCCATAAGTTAATAGTGGCTGATGGCAAGATTGCTGATGCGGGGAAGGTGTTTAGCCAAATGTTCTTTGATGCTGGGGCACACTTTACCACGGTCATTTGTGCAGCGGAATTGCCCACCGTAAAGGATGTGGTAACTGTGGGTAACAGTTACACGCCCATAAAAGAAACCCAGGTGGAAATGACCAGTAACTTCACTTGGGAGCAGGTAACGCAGTGA", "aa": "MALPLIQIALDNLSLASALNDLAKVGDAVDVIEVGTILLTAEGVNAVKEIAKRYPHKLIVADGKIADAGKVFSQMFFDAGAHFTTVICAAELPTVKDVVTVGNSYTPIKETQVEMTSNFTWEQVTQ", "aa_hexdigest": "67d4e42584165d37aaba7faed86f9005", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00387B8401", "query_cov": 0.8095238095238095, "subject_cov": 0.9902912621359223, "identity": 0.5, "score": 107.0, "evalue": 6.18e-28, "valid": false, "product": "orotidine 5'-phosphate decarboxylase / HUMPS family protein", "uniref50_id": "UniRef50_UPI00387B8401" }, "pscc": { "uniref50_id": "UniRef50_UPI00387B8401", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00387B8401" ], "product": "orotidine 5'-phosphate decarboxylase / HUMPS family protein" }, "genes": [], "id": "APKECPOBEN_1070", "locus": "GCA002090215_01066" }, { "type": "cds", "sequence": "contig_1", "start": 601018, "stop": 601293, "strand": "-", "frame": 1, "gene": "ulaC", "product": "Ascorbate-specific PTS system EIIA component", "db_xrefs": [ "COG:COG1762", "COG:GT", "GO:0005737", "GO:0009401", "GO:0016301", "SO:0001217", "UniRef:UniRef50_P75292", "UniRef:UniRef90_P75292" ], "nt": "ATGCCTCATGCCCAGAATAACGGTACGGTACAGCAAAACTGCTTCTCTTTAGTGACCCTAAAGGAACCAGTTTACTTTGACAACGATCCCCGCCCGGTGCGCTTGTTAATTGGATTAGCGGCTACCAGCGCTGCCATTCATACGACAGAAGCTTTACCACAAATTGCGGCCCTAGTAGAAGATACTCAAGTGGTTAAGGACTTGTTGGACTGTTGTGATGAGACCAGCTTGTTTGCTGTGATTGATCGGGTTAACTTACACAAATATTTAACTTAA", "aa": "MPHAQNNGTVQQNCFSLVTLKEPVYFDNDPRPVRLLIGLAATSAAIHTTEALPQIAALVEDTQVVKDLLDCCDETSLFAVIDRVNLHKYLT", "aa_hexdigest": "2ccbafedad258642cb98db42bf2d82eb", "start_type": "ATG", "rbs_motif": null, "genes": [ "ulaC" ], "truncated": "3-prime", "pseudogene": { "start": 601018, "stop": 601497, "observations": { "mutation": [ 601425 ], "stop": [ 601425 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75292", "query_cov": 1.0, "subject_cov": 0.5723270440251572, "identity": 1.0, "score": 186.0, "evalue": 7.85e-59, "gene_start": 1, "gene_end": 91, "reference_start": 69, "reference_end": 159, "reference_sequence": "MTKLDFKAVLKQHHTVRLHQTAQDWKQAIQLCIHPLIAAKLVQPAYFDDILKSVAQYGPYFIIAENVAMPHAQNNGTVQQNCFSLVTLKEPVYFDNDPRPVRLLIGLAATSAAIHTTEALPQIAALVEDTQVVKDLLDCCDETSLFAVIDRVNLHKYLT" }, "paralog": false, "identity": 0.9937106918238994, "score": 316.0, "evalue": 1.22e-112, "description": "Internal stop codon at 601425. Nonsense mutation around 601425.", "psc": { "uniref90_id": "UniRef90_P75292", "gene": "ulaC", "product": "Ascorbate-specific PTS system EIIA component", "uniref50_id": "UniRef50_P75292", "cog_id": "COG1762", "cog_category": "GT", "go_ids": [ "GO:0005737", "GO:0016301", "GO:0009401" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75292", "UniRef:UniRef50_P75292", "COG:COG1762", "COG:GT", "GO:0005737", "GO:0016301", "GO:0009401" ] }, "pscc": { "uniref50_id": "UniRef50_P75292", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75292" ], "product": "Ascorbate-specific PTS system EIIA component" } }, "id": "APKECPOBEN_1071", "locus": "GCA002090215_01067" }, { "type": "cds", "sequence": "contig_1", "start": 601499, "stop": 601786, "strand": "-", "frame": 3, "gene": "ulaB", "product": "Ascorbate-specific PTS system EIIB component", "db_xrefs": [ "COG:COG3414", "COG:G", "EC:2.7.1.194", "GO:0005737", "GO:0008982", "GO:0009401", "GO:0016301", "RefSeq:WP_010874851.1", "SO:0001217", "UniParc:UPI00001329E2", "UniRef:UniRef100_Q9EXD8", "UniRef:UniRef50_Q9EXD8", "UniRef:UniRef90_Q9EXD8" ], "nt": "ATGGAAAATAAGAACTTACACATTATTGCCGCTTGTGGTAACGGCATGGGTACGAGCATGCTAATCAAGATTAAGGTTGAAAAGATTATGAAGGAGTTGGGCTACACCGCCAAAGTGGAAGCTTTGTCAATGGGACAAACCAAGGGGATGGAGCACAGCGCTGACATTATTATTTCCTCGATTCACTTAACGAGTGAGTTTAACCCGAATGCTAAGGCCAAGATTGTTGGGGTGTTAAACCTGATGGATGAAAACGAAATTAAGCAAGCGTTAAGTAAGGTGCTTTAA", "aa": "MENKNLHIIAACGNGMGTSMLIKIKVEKIMKELGYTAKVEALSMGQTKGMEHSADIIISSIHLTSEFNPNAKAKIVGVLNLMDENEIKQALSKVL", "aa_hexdigest": "b67d7a5b75a72095495208b826ecbf40", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001329E2", "ncbi_nrp_id": "WP_010874851.1", "uniref100_id": "UniRef100_Q9EXD8", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001329E2", "RefSeq:WP_010874851.1", "UniRef:UniRef100_Q9EXD8" ] }, "ips": { "uniref100_id": "UniRef100_Q9EXD8", "uniref90_id": "UniRef90_Q9EXD8" }, "psc": { "uniref90_id": "UniRef90_Q9EXD8", "gene": "ulaB", "product": "Ascorbate-specific PTS system EIIB component", "ec_ids": [ "2.7.1.194" ], "uniref50_id": "UniRef50_Q9EXD8", "cog_id": "COG3414", "cog_category": "G", "go_ids": [ "GO:0005737", "GO:0016301", "GO:0008982", "GO:0009401" ] }, "pscc": { "uniref50_id": "UniRef50_Q9EXD8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9EXD8" ], "product": "Ascorbate-specific PTS system EIIB component" }, "genes": [ "ulaB" ], "id": "APKECPOBEN_1072", "locus": "GCA002090215_01068" }, { "type": "cds", "sequence": "contig_1", "start": 601789, "stop": 601905, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGCTACCGTTGGTTTAATCTTGCTGGCACAAATTATTGACAATGGTAAGCAAACTAAAGTAACTAAATTACAACAATTACTCAAAATTAACCCACAATTAGATTTAAATAACTAA", "aa": "MATVGLILLAQIIDNGKQTKVTKLQQLLKINPQLDLNN", "aa_hexdigest": "ea7e76d9ecf550aeada399359f77c771", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4201.970200000001, "isoelectric_point": 9.524575233459473 }, "id": "APKECPOBEN_1073", "locus": "GCA002090215_01069" }, { "type": "cds", "sequence": "contig_1", "start": 601948, "stop": 602661, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTGGCCAGCTTAATTGCCATTATGACTGGGGTAAGGATGTTTATCACTGAGTTACAACAGGCGTTTCAAGGTATTAGTGAAAAGGTAGTACCAGGGGCCGTGGTAGCGGTTGATATTGCAGCTGTTTATGGGTTTTCAATTAACTCCGTGACCTTTGGGTTTTTAAGTGGGGTCATTGGTCAGTTTTTAGCTGTTGCCATTATGGCTGGCATTAGCTATATTCCGGGTAACCAGTTTAGCTTTGTGGCGATTCCTTTGTTTATTACACTCTTCTTTAACAGTGGTGCCTTTGGCGTGTATGCTAATGCCGAGGGTGGTTGGAAGGCGGCCTTACTGCTCCCGGGTATTATTGGCTTTTTAGAAATTATTGTGATTTCATTTGCGCTGCGCACCGTATCAAACGCTTACCAGGCTTCGGCACTGTTAGATGCTAAACAAAGCTTTGATTTAAAGGCGTTAATGGGTAATAGTTTGGACAATAACAACGGTTCGGCATTAAAAACAGCCGTAGAAAAGGTTGTAAATGCTACTGGTGTTAACCGGATAACCGAAGCGCAAAGCTTTGTTAAATCCGATAGCTTTAACAGTTTAAAAGCTGTTAACAGTACGCTAGCACAAGCAATTGAGTGGATCAGTAAAAGTGCATCACCCGTTGACAACGGTTTTATTGGAATGGCTGACTGGAATCTTTACTTTGGGTTGTTAGTTTGA", "aa": "MVASLIAIMTGVRMFITELQQAFQGISEKVVPGAVVAVDIAAVYGFSINSVTFGFLSGVIGQFLAVAIMAGISYIPGNQFSFVAIPLFITLFFNSGAFGVYANAEGGWKAALLLPGIIGFLEIIVISFALRTVSNAYQASALLDAKQSFDLKALMGNSLDNNNGSALKTAVEKVVNATGVNRITEAQSFVKSDSFNSLKAVNSTLAQAIEWISKSASPVDNGFIGMADWNLYFGLLV", "aa_hexdigest": "fa2c958b629bea6f4d56c8ada9842548", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 25030.702300000004, "isoelectric_point": 5.06540012359619 }, "id": "APKECPOBEN_1074", "locus": "GCA002090215_01070" }, { "type": "cds", "sequence": "contig_1", "start": 602719, "stop": 603198, "strand": "-", "frame": 1, "gene": null, "product": "PTS ascorbate transporter subunit IIC", "db_xrefs": [ "KEGG:K03475", "SO:0001217", "UniRef:UniRef50_UPI002244F519", "UniRef:UniRef90_UPI002244F519" ], "nt": "ATGGTTACTGGACACGTTATGTTGCAACAAGCAGCGGTAGTTACTACGCTGTTCTATATTGTCTTGTTCCGCCAAATTCCGTTGTTGGGTACGGGCATTGCTTACGGTGCCCAAGCCGGTCTAGTGATTATTAGTGGTATCTTTTTGGGAGTTTACTGGTCTACGGCCTCTACGGGTACCTATTTAGTGACCAATAAGGTAACCAATAATGCTGGTTTTTCGATTGGTCACCAGCAGATGTTAGGGATTATGACGGTTGCTAAATTGGGCAAGTACTTTGGTGACAAAAACGATAGTGCCGAGCACAAAAAACTGCCTAAGGCCCTAAAGATCTTTGAAGATAATATCTTCACCCAAACGATCATTATCCTTAGTTTGTTTGTGGTGTTGTTTATTGTGATTTTGGCTAGTTACAAGGGTGATAAACCACTGTTAATTAACTGGGACAAATTTGGTGCCATTAATGGTTTAGAGATTTGA", "aa": "MVTGHVMLQQAAVVTTLFYIVLFRQIPLLGTGIAYGAQAGLVIISGIFLGVYWSTASTGTYLVTNKVTNNAGFSIGHQQMLGIMTVAKLGKYFGDKNDSAEHKKLPKALKIFEDNIFTQTIIILSLFVVLFIVILASYKGDKPLLINWDKFGAINGLEI", "aa_hexdigest": "c5228fc0191eff5d5115782c24e927bd", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 602763, "stop": 603498, "observations": { "insertion": [ 602772 ], "mutation": [ 603219 ], "stop": [ 603219 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI002244F519", "query_cov": 0.8930817610062893, "subject_cov": 0.42388059701492536, "identity": 1.0, "score": 270.0, "evalue": 1.29e-88, "gene_start": 1, "gene_end": 142, "reference_start": 192, "reference_end": 333, "reference_sequence": "MLNKIKLQQKRKLIIGWSVFALINLVVILLTVLLRAGLPNLVSKGVTPFSAQAFGDAFIFLITRVYLDNFLRQPALLLGVITLIGYLALGRGGVQSVVGALKTVIGFILLSIGSGVLVSTARPVFDTIKGLGGTGVVLLDPYFSLASANDFFTNSFLNNDYVSLIAFSLLVGFIVNIIFVGLKRWTNTNSIMVTGHVMLQQAAVVTTLFYIVLFRQIPLLGTGIAYGAQAGLVIISGIFLGVYWSTASTGTYLVTNKVTNNAGFSIGHQQMLGIMTVAKLGKYFGDKNDSAEHKKLPKALKIFEDNIFTQTIIILSLFVVLFIVILASYKGDKHC" }, "paralog": false, "identity": 0.9959016393442623, "score": 464.0, "evalue": 3.21e-167, "description": "Internal stop codon at 603219. Frameshift due to insertion around 602772. Nonsense mutation around 603219.", "psc": { "uniref90_id": "UniRef90_UPI002244F519", "product": "PTS ascorbate transporter subunit IIC", "uniref50_id": "UniRef50_UPI002244F519", "kegg_orthology_id": "K03475", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI002244F519", "UniRef:UniRef50_UPI002244F519", "KEGG:K03475" ] }, "pscc": { "uniref50_id": "UniRef50_UPI002244F519", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002244F519" ], "product": "PTS ascorbate transporter subunit IIC" } }, "id": "APKECPOBEN_1075", "locus": "GCA002090215_01071" }, { "type": "cds", "sequence": "contig_1", "start": 603217, "stop": 603696, "strand": "-", "frame": 1, "gene": null, "product": "PTS ascorbate transporter subunit IIC", "db_xrefs": [ "KEGG:K03475", "SO:0001217", "UniRef:UniRef50_UPI002244F519", "UniRef:UniRef90_UPI002244F519" ], "nt": "GTGGTAATTCTCTTGACAGTATTACTGCGTGCAGGATTACCGAACTTAGTTAGTAAGGGAGTAACCCCGTTTTCGGCCCAAGCCTTTGGTGATGCCTTTATCTTTTTAATTACGCGGGTATACCTCGATAACTTTTTGCGTCAACCAGCGCTATTGTTAGGTGTTATTACCCTAATTGGTTACCTTGCTTTAGGTCGTGGTGGGGTGCAATCGGTGGTGGGGGCACTAAAGACCGTAATTGGCTTTATCCTGTTAAGCATTGGTAGTGGCGTGTTGGTGTCCACCGCCCGTCCCGTTTTTGACACGATTAAGGGTTTAGGGGGTACTGGTGTGGTACTATTGGATCCTTACTTTTCCTTAGCATCAGCGAACGATTTCTTTACGAACAGTTTCTTGAACAATGACTATGTGTCCTTGATTGCCTTTTCGCTGTTAGTGGGCTTTATTGTCAACATTATCTTTGTGGGGTTAAAGCGTTGA", "aa": "MVILLTVLLRAGLPNLVSKGVTPFSAQAFGDAFIFLITRVYLDNFLRQPALLLGVITLIGYLALGRGGVQSVVGALKTVIGFILLSIGSGVLVSTARPVFDTIKGLGGTGVVLLDPYFSLASANDFFTNSFLNNDYVSLIAFSLLVGFIVNIIFVGLKR", "aa_hexdigest": "727f362a7aa2c1218afdd2e34c123151", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 602914, "stop": 603771, "observations": { "mutation": [ 603219, 603723 ], "stop": [ 603219, 603723 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI002244F519", "query_cov": 0.9937106918238994, "subject_cov": 0.4716417910447761, "identity": 1.0, "score": 288.0, "evalue": 9.59e-96, "gene_start": 2, "gene_end": 159, "reference_start": 27, "reference_end": 184, "reference_sequence": "MLNKIKLQQKRKLIIGWSVFALINLVVILLTVLLRAGLPNLVSKGVTPFSAQAFGDAFIFLITRVYLDNFLRQPALLLGVITLIGYLALGRGGVQSVVGALKTVIGFILLSIGSGVLVSTARPVFDTIKGLGGTGVVLLDPYFSLASANDFFTNSFLNNDYVSLIAFSLLVGFIVNIIFVGLKRWTNTNSIMVTGHVMLQQAAVVTTLFYIVLFRQIPLLGTGIAYGAQAGLVIISGIFLGVYWSTASTGTYLVTNKVTNNAGFSIGHQQMLGIMTVAKLGKYFGDKNDSAEHKKLPKALKIFEDNIFTQTIIILSLFVVLFIVILASYKGDKHC" }, "paralog": false, "identity": 0.9929824561403509, "score": 537.0, "evalue": 3.96e-196, "description": "Internal stop codon at 603219, 603723. Nonsense mutation around 603219, 603723.", "psc": { "uniref90_id": "UniRef90_UPI002244F519", "product": "PTS ascorbate transporter subunit IIC", "uniref50_id": "UniRef50_UPI002244F519", "kegg_orthology_id": "K03475", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI002244F519", "UniRef:UniRef50_UPI002244F519", "KEGG:K03475" ] }, "pscc": { "uniref50_id": "UniRef50_UPI002244F519", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002244F519" ], "product": "PTS ascorbate transporter subunit IIC" } }, "id": "APKECPOBEN_1076", "locus": "GCA002090215_01072" }, { "type": "cds", "sequence": "contig_1", "start": 603771, "stop": 603914, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W6G9", "UniRef:UniRef90_A0AB38W6G9" ], "nt": "GTGGGAGTTTCCGATGCGGCCATTAACACCATCTTGGTCGAAAATCTCGCTGAAATCCTGGCCTTTGATGCACCGCGGCAGTTTAATCCCAAGGCTGTGCATCCACGGGTATTAGCACTTAAAAAACAACTAAAAATAGAGTAA", "aa": "MGVSDAAINTILVENLAEILAFDAPRQFNPKAVHPRVLALKKQLKIE", "aa_hexdigest": "c87fb012b4fe664ccc56e721b6a89d51", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0AB38W6G9", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.9570000000000001, "score": 87.8, "evalue": 3.54e-22, "valid": true, "uniref50_id": "UniRef50_A0AB38W6G9" }, "pscc": { "uniref50_id": "UniRef50_A0AB38W6G9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W6G9" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5184.0652, "isoelectric_point": 8.212708091735841 }, "id": "APKECPOBEN_1077", "locus": "GCA002090215_01073" }, { "type": "cds", "sequence": "contig_1", "start": 603980, "stop": 604135, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002FEEE66", "UniRef:UniRef100_A0AB38W6Q4", "UniRef:UniRef50_A0AB38W6Q4", "UniRef:UniRef90_A0AB38W6Q4" ], "nt": "GTGACCCTGTGTTTTGATGGGCCCGATCGGGTGAAGTATTACCCCGATTGTTTATTGGCTAAACACATTAAATACTTAGTGGACTTGGGCTTTGTTAAACACATTACCCTGGCCTTAGATGCGGGGCGGGTACTGTACCAAAACACTATGGTTTAG", "aa": "MTLCFDGPDRVKYYPDCLLAKHIKYLVDLGFVKHITLALDAGRVLYQNTMV", "aa_hexdigest": "4c2ee55de2c9cbfbff0f2f645c7c6ee5", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0002FEEE66", "uniref100_id": "UniRef100_A0AB38W6Q4", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002FEEE66", "UniRef:UniRef100_A0AB38W6Q4" ] }, "ips": { "uniref100_id": "UniRef100_A0AB38W6Q4", "uniref90_id": "UniRef90_A0AB38W6Q4" }, "psc": { "uniref90_id": "UniRef90_A0AB38W6Q4", "uniref50_id": "UniRef50_A0AB38W6Q4" }, "pscc": { "uniref50_id": "UniRef50_A0AB38W6Q4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W6Q4" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5859.9022, "isoelectric_point": 7.79804744720459 }, "id": "APKECPOBEN_1078", "locus": "GCA002090215_01074" }, { "type": "cds", "sequence": "contig_1", "start": 604199, "stop": 604630, "strand": "-", "frame": 3, "gene": null, "product": "Phosphotriesterase-related protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAV5N8W9", "UniRef:UniRef90_A0AAV5N8W9" ], "nt": "ATGGATCCACCGAATGTGGGCCGTGATGTCAAACGGATGGTAGCGATTGCAGAACAACTAAAGGGCAAGCTGAACATTATTATGGCCACTGGTTTTCACAAGGCTGCTTTCTATGACAAGGGAAGTTCTTGGTTAGCACAAGTGCCAGTTAATGAAATTGTGCCGATGTTGGTCGCTGAAATAGAAGAGGGTATGGATTTGTATAACTATTCCGGTCCCGTGGTTAAACGGGGCAAGGCTAAGGCAGGCATTATTAAAGCTGGTACGGGCTATGCTGCGATTGACCGGTTGGAATTAAAGGCCTTGGAAGCAGTCGCGATTACTTCGATTACTACTGGTGCTCCCGTACTCGTGCACACCCAACTCGGTACCATGGCATATGAAGCAGCACAACACCTGATTGATTTTGGCGTTAATCCGCGGAAAATTTAA", "aa": "MDPPNVGRDVKRMVAIAEQLKGKLNIIMATGFHKAAFYDKGSSWLAQVPVNEIVPMLVAEIEEGMDLYNYSGPVVKRGKAKAGIIKAGTGYAAIDRLELKALEAVAITSITTGAPVLVHTQLGTMAYEAAQHLIDFGVNPRKI", "aa_hexdigest": "5a71c4167830b9601d8ce4e62efaa19e", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0AAV5N8W9", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.993, "score": 278.0, "evalue": 7.21e-95, "valid": true, "product": "Phosphotriesterase-related protein", "uniref50_id": "UniRef50_A0AAV5N8W9" }, "pscc": { "uniref50_id": "UniRef50_A0AAV5N8W9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAV5N8W9" ], "product": "Phosphotriesterase-related protein" }, "genes": [], "id": "APKECPOBEN_1079", "locus": "GCA002090215_01075" }, { "type": "cds", "sequence": "contig_1", "start": 604664, "stop": 604825, "strand": "-", "frame": 3, "gene": null, "product": "putative protein MPN_497", "db_xrefs": [ "GO:0008270", "GO:0009056", "GO:0016787", "SO:0001217", "UniParc:UPI0001DD6406", "UniRef:UniRef100_P75290", "UniRef:UniRef50_Q4A724", "UniRef:UniRef90_P75290" ], "nt": "ATGAAAAGATTTGTAAGAACAGTTTTAGGGGACATTGACCCTAAGGATTTAGGTATTTGTGACTGTCACGATCATTTAATTAAGAATTGGGGTCCGGAAGCCAAAGAGCACCCTGATTTTGTGATGTTATCGAATGAAGCTGCTATTAAAGAGTGTTTGTAA", "aa": "MKRFVRTVLGDIDPKDLGICDCHDHLIKNWGPEAKEHPDFVMLSNEAAIKECL", "aa_hexdigest": "72db58d0e68b6a7a87116caca0a28e08", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD6406", "uniref100_id": "UniRef100_P75290", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD6406", "UniRef:UniRef100_P75290" ] }, "ips": { "uniref100_id": "UniRef100_P75290", "uniref90_id": "UniRef90_P75290" }, "psc": { "uniref90_id": "UniRef90_P75290", "product": "Uncharacterized protein MPN_497", "uniref50_id": "UniRef50_Q4A724", "go_ids": [ "GO:0016787", "GO:0008270", "GO:0009056" ] }, "pscc": { "uniref50_id": "UniRef50_Q4A724", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A724" ], "product": "Phospho-furanose lactonase" }, "genes": [], "id": "APKECPOBEN_1080", "locus": "GCA002090215_01076" }, { "type": "cds", "sequence": "contig_1", "start": 604941, "stop": 605669, "strand": "+", "frame": 3, "gene": "ulaF", "product": "putative L-ribulose-5-phosphate 4-epimerase UlaF", "db_xrefs": [ "COG:COG0235", "COG:EG", "EC:5.1.3.4", "GO:0005829", "GO:0008270", "GO:0008742", "GO:0016832", "GO:0019323", "GO:0019852", "GO:0019854", "RefSeq:WP_010874854.1", "SO:0001217", "UniParc:UPI00001358F9", "UniRef:UniRef100_P75289", "UniRef:UniRef50_P75289", "UniRef:UniRef90_P75289" ], "nt": "ATGGATCAAAAAATGATAAATGACCTCAAAGAACAGGTTTTTCAAACGAATTTATTACTACCCAAATATGGCTTAGTCATCCACACTTGGGGTAATGTTTCGATGATTGCCCCGAACCGTCAGTTCTTTGTCATTAAACCAAGTGGTGTGAGCTATGACAAGATGCGTGCCCAAGATATGGTAGTGGTCGATTTGGACAACAATGTTCTAGATACCAACGGCCTCAAACCATCAAGTGATACCCCAACCCATGCGTTAATGTACAAACATTGTCCAGATATTAAAGCTATTGTGCACACCCACTCTACTTTCGCGACATCGTTTGCTCAAGCCGATAAGCCGATTCCTTGTCTTGGTACTACCCATGCTGATAACTTCTTTGGACCAATCCCGTGTACTAGGGCTTTAAGTGACAGTGAAATTAATGGAGCTTATGAACATAATACTGGTTTAGTCATTCTGGAACACCTCAAAAACAACCAGGTTGATGTCAATGCTTGTGCGGCCATCTTAGTCAAAGAACACGGTTCGTTTGTTTGGTCCAATAAGAATGGCAAGGATGCAGTGGACCGGGCGTTAACTTTAGAACAAGTGGCCCAAATGGCCTTATATACCCAAATGATCAATCCCCACATGAAAGAAGCAAACCCAGCGTTACAACAAAAGCACTATAACCGCAAACATGGTAAAGATGCTTACTATGGACAAGATACTAAGCAAGAAGATTAA", "aa": "MDQKMINDLKEQVFQTNLLLPKYGLVIHTWGNVSMIAPNRQFFVIKPSGVSYDKMRAQDMVVVDLDNNVLDTNGLKPSSDTPTHALMYKHCPDIKAIVHTHSTFATSFAQADKPIPCLGTTHADNFFGPIPCTRALSDSEINGAYEHNTGLVILEHLKNNQVDVNACAAILVKEHGSFVWSNKNGKDAVDRALTLEQVAQMALYTQMINPHMKEANPALQQKHYNRKHGKDAYYGQDTKQED", "aa_hexdigest": "de481f86e0ac1819ae64baf5a4e9813a", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001358F9", "ncbi_nrp_id": "WP_010874854.1", "uniref100_id": "UniRef100_P75289", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001358F9", "RefSeq:WP_010874854.1", "UniRef:UniRef100_P75289" ] }, "ips": { "uniref100_id": "UniRef100_P75289", "uniref90_id": "UniRef90_P75289" }, "psc": { "uniref90_id": "UniRef90_P75289", "gene": "ulaF", "product": "Probable L-ribulose-5-phosphate 4-epimerase UlaF", "ec_ids": [ "5.1.3.4" ], "uniref50_id": "UniRef50_P75289", "cog_id": "COG0235", "cog_category": "EG", "go_ids": [ "GO:0005829", "GO:0016832", "GO:0008742", "GO:0008270", "GO:0019854", "GO:0019852", "GO:0019323" ] }, "pscc": { "uniref50_id": "UniRef50_P75289", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75289" ], "product": "Probable L-ribulose-5-phosphate 4-epimerase UlaF" }, "genes": [ "ulaF" ], "id": "APKECPOBEN_1081", "locus": "GCA002090215_01077" }, { "type": "cds", "sequence": "contig_1", "start": 605623, "stop": 606114, "strand": "+", "frame": 1, "gene": null, "product": "MPN499 family protein", "db_xrefs": [ "RefSeq:WP_010874855.1", "SO:0001217", "UniParc:UPI000013AB8A", "UniRef:UniRef100_P75288", "UniRef:UniRef50_P75288", "UniRef:UniRef90_P75288" ], "nt": "ATGGTAAAGATGCTTACTATGGACAAGATACTAAGCAAGAAGATTAAAGTGAACTGGTTGGGCGGGGTGTTTTGGTTATTACCTAACTTACTCGATCTGTTTTCCGCTAGCAAACGTAAAGCGAGTGTGCGACCCTACCAAAGCTTATTGGAACTGGTTCAAGAAAACTTCTTGAACCGGTATGACCTCGTGCACTTTAGCTTTAATGGTGACCATGACTTCTTTCACTTTAATGATTTACAAGCCATTAGGAGCTTTAACTTTACGATAGAAGAGGAACAACTAGGAGCAATGCAACCAGATGAGGTTTTACTGTTTGAACCTGTGGATCGTGTAACAGTGGAATTAGATCAAAAGGGTTTGAGTTTAATCCACAGTGGCAAAGCTTTTTGTGCTAGTGCAAACTACTTTAAGCACTGGCTAAAACGCGTACCACAGCAAGACAAAGTGACCCTAGTGTGGCGAAAATCAGGTTTTGAATTAAAGCAGTAG", "aa": "MVKMLTMDKILSKKIKVNWLGGVFWLLPNLLDLFSASKRKASVRPYQSLLELVQENFLNRYDLVHFSFNGDHDFFHFNDLQAIRSFNFTIEEEQLGAMQPDEVLLFEPVDRVTVELDQKGLSLIHSGKAFCASANYFKHWLKRVPQQDKVTLVWRKSGFELKQ", "aa_hexdigest": "9c705784d6f0e311001497b1cc97e276", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013AB8A", "ncbi_nrp_id": "WP_010874855.1", "uniref100_id": "UniRef100_P75288", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013AB8A", "RefSeq:WP_010874855.1", "UniRef:UniRef100_P75288" ] }, "ips": { "uniref100_id": "UniRef100_P75288", "uniref90_id": "UniRef90_P75288" }, "psc": { "uniref90_id": "UniRef90_P75288", "product": "MPN499 family protein", "uniref50_id": "UniRef50_P75288" }, "pscc": { "uniref50_id": "UniRef50_P75288", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75288" ], "product": "Uncharacterized protein MPN_499" }, "genes": [], "id": "APKECPOBEN_1082", "locus": "GCA002090215_01078" }, { "type": "cds", "sequence": "contig_1", "start": 606103, "stop": 606459, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTCGAGGGTGGGTCATTTGGGAATATTGTTCCCCGTAGTCGTCGTCTGACTGCCCCCGAGGGTGCCCTGGACATTCGCTTTGAGGCCAACGCCGACATTCGAAAACGTCGGGAGAAAGGAACGCGAACCACTTGTGTTCGTACTAGTTCCGCTGGACGACCCCGCGCTGGTTTGAGGCGCGGTTATATCATCCACACACCGGTGGTTATTCCCATACCTGTACCCATTCCCAATCCAGGGAATATAGTCAAACACCTGGTCGAGCACCGACCCCGCGAGGTTGAACCCGGTGGAGAAACGGGAAAGCGTGGGGGAGAAGTGCTATATAGTATTAGAGTAATTCTACTGCTTTAA", "aa": "MVEGGSFGNIVPRSRRLTAPEGALDIRFEANADIRKRREKGTRTTCVRTSSAGRPRAGLRRGYIIHTPVVIPIPVPIPNPGNIVKHLVEHRPREVEPGGETGKRGGEVLYSIRVILLL", "aa_hexdigest": "241901665b5a6d1f46a27eb37e73aa84", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12977.925399999987, "isoelectric_point": 11.206748771667481 }, "id": "APKECPOBEN_1083", "locus": "GCA002090215_01079" }, { "type": "cds", "sequence": "contig_1", "start": 606829, "stop": 607119, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTGGTTAATAAGTCCGGCCAAGATGATAACAGTAAGTTTAAGGCGGAGGACCAAAAATGGTCCTACACCGACTTACAGTCGGACCAAACCAAACTGAACCTCCCCGCTTACGGCGAGGTGAATGGGTTGTTGAATCCGGCGTTGGTGGAAACCTATTTTGGGAACACGCGAGCGAGTGGTTCGGGGTCCAACACGACCAGTTCACCCGGTATCGGTTTTAAAATTCCCGAACAAAGTGGCACAAACACAACGTCGAAGGCTGTGCTGATCACCCCCGGGTTGGCTTGA", "aa": "MLVNKSGQDDNSKFKAEDQKWSYTDLQSDQTKLNLPAYGEVNGLLNPALVETYFGNTRASGSGSNTTSSPGIGFKIPEQSGTNTTSKAVLITPGLA", "aa_hexdigest": "890f22afdbdbb58513e9f1ad165ff405", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10171.074699999988, "isoelectric_point": 5.170722770690916 }, "id": "APKECPOBEN_1084", "locus": "GCA002090215_01080" }, { "type": "cds", "sequence": "contig_1", "start": 607342, "stop": 607845, "strand": "+", "frame": 1, "gene": null, "product": "adhesin", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75593", "UniRef:UniRef90_UPI001330D5D0" ], "nt": "GTGGAGAGTGTGTGGGATTTCAAGGGGGTGTGGGCGGATCAAGCTCAGTTGGCCGCGCAAGCAGCTACAAGTAGTACCACCACCACCGCAACAGGGGCTACCTTACCGGAGCACCCGAATGCCCTCGCGTACCAAATTAGCTATACCGACAAGGATTCGTACAAGGCTTCCACTCAAGGTTCGGGTCAAACCAATTCCCAAAACAATTCGCCCTACCTCCATTTTATTAAACCTAAGAAAGTCGAAAGCACGACCCAACTCGACCAGGGCTTAAAAAACCTGTTGGACCCCAACCAGGTTCGCACCAAGCTGCGCCAAAGCTTTGGTACAGACCATTCCACCCAGCCCCAGCCCCAATCGCTCAAAACAACGACACCGGTATTTGGGAGGAGTAGTGGTAACCTCAGTAGTGTGTTTAGTGGTGGGGGTGCTGGAGGGGGTTCTTCAGGCTCAGGTCAATCTGGCGTGGATCTCTCCCCCGTTGAACGGGTGAGTGGTCACTAA", "aa": "MESVWDFKGVWADQAQLAAQAATSSTTTTATGATLPEHPNALAYQISYTDKDSYKASTQGSGQTNSQNNSPYLHFIKPKKVESTTQLDQGLKNLLDPNQVRTKLRQSFGTDHSTQPQPQSLKTTTPVFGRSSGNLSSVFSGGGAGGGSSGSGQSGVDLSPVERVSGH", "aa_hexdigest": "a24e70da1334e338a997e1439fde5de8", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 607042, "stop": 607845, "observations": { "mutation": [ 607117, 607300 ], "stop": [ 607117, 607300 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001330D5D0", "query_cov": 1.0, "subject_cov": 0.46005509641873277, "identity": 0.946, "score": 292.0, "evalue": 9.82e-97, "gene_start": 1, "gene_end": 167, "reference_start": 197, "reference_end": 363, "reference_sequence": "ALSFTNKNNAQRAQLFLRGLLGSIPVLVNKSGQDDNSKFKAEDQKWSYTDLQSDQTKLNLPAYGEVNGLLNPALVETYFGNTRASGSGSNTTSSPGIGFKIPEQSGTNTTSKAVLITPGLAWTPQDVGNLVVSGTSFSFQLGGWLVTFTDFIKPRAGYLGLQLTGLDVSEATQRELIWAKRPWAAFRGSWVNRLGRVESVWDFKGVWADQAQLAAQAATSSTTTTATGATLPEHPNALAYQISYTDKDLYKASTQGSGQTNSQNNSPYLHFIKPKKVESTTQLDQGLKNLLDPNQVRTKLRQSFGTDHSTQPQPQSLKTTTPVFGTSSGNLSSVFSGRGAGGGSSGSGQSGVDLSPVERVSGH" }, "paralog": false, "identity": 0.9588014981273408, "score": 495.0, "evalue": 5.72e-179, "description": "Internal stop codon at 607117, 607300. Nonsense mutation around 607117, 607300.", "psc": { "uniref90_id": "UniRef90_UPI001330D5D0", "product": "adhesin", "uniref50_id": "UniRef50_P75593", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001330D5D0", "UniRef:UniRef50_P75593" ] }, "pscc": { "uniref50_id": "UniRef50_P75593", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75593" ], "product": "Putative adhesin P1-like protein MPN_099" } }, "id": "APKECPOBEN_1085", "locus": "GCA002090215_01081" }, { "type": "cds", "sequence": "contig_1", "start": 607912, "stop": 608211, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD640A", "UniRef:UniRef100_A0A0H3DNZ5", "UniRef:UniRef50_A0A0H3DNZ5", "UniRef:UniRef90_A0A0H3DNZ5" ], "nt": "GTGTCTTTACCAACTTTAAGCAATTTAGTGAACGAATACCCAAAAAGTATGAAATTAGTTAAATTAGCGAGGCTTCACCGCTTTTTAAAACAAGACCAATTGCAACTTGTTCCTTTGTTTAGTCCAAACTTTTTGCAAGTTTTGTTGGCGCTTCTCAATAAACTGGTCAATTTATTTAGTGAAAACACAACAATGAAAACAAATAAAAAATACGCCTTTGCGGCGTTGCTAATGCAAGCTCAAGCTAAGCTCCCAAATTCATTTAAATTCTTTCTTTCAAAGAAAGGAGGTATGGCTTAA", "aa": "MSLPTLSNLVNEYPKSMKLVKLARLHRFLKQDQLQLVPLFSPNFLQVLLALLNKLVNLFSENTTMKTNKKYAFAALLMQAQAKLPNSFKFFLSKKGGMA", "aa_hexdigest": "9cdb1adeccaa0056dddaece30460748d", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD640A", "uniref100_id": "UniRef100_A0A0H3DNZ5", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD640A", "UniRef:UniRef100_A0A0H3DNZ5" ] }, "ips": { "uniref100_id": "UniRef100_A0A0H3DNZ5", "uniref90_id": "UniRef90_A0A0H3DNZ5" }, "psc": { "uniref90_id": "UniRef90_A0A0H3DNZ5", "uniref50_id": "UniRef50_A0A0H3DNZ5" }, "pscc": { "uniref50_id": "UniRef50_A0A0H3DNZ5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0H3DNZ5" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11274.444899999984, "isoelectric_point": 10.390707969665527 }, "id": "APKECPOBEN_1086", "locus": "GCA002090215_01082" }, { "type": "cds", "sequence": "contig_1", "start": 608379, "stop": 608801, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01519.22" ], "nt": "GTGAACACGGAATACGTAACGCGTAAAGAGTTTAACGAGTACAAAAACTCAAACGACCAACGACTTACAAAGATTGAAACCACTTTGGCCGCCCAAGGTGAACAAATCAACAAATTGACTCAAACTGTTGAAAAACAAGGCGAGCAAATCAATCAATTAGTTCAAGTTGTGCTTCTTCACGGTGAACAAATCAACAAATTAACTCAAACTGTTGAAAAACAGGGCGAGCAAATTAAAGAACTTCAAATCGAGCAAAAAGCTCAGGGCGAAACCTTAAAGCTAATCTTGCAAACGCTGCAAAAGATGAGCGATCGCTTGGACAAGATGGAAGTAAAAATGGACAAAATGGAAGTAAAAATGGACAAAATGGAAGTAAAAATGGACAAAATGGAAAAAAGAATAGACAAACTTGAGTCCAAATAA", "aa": "MNTEYVTRKEFNEYKNSNDQRLTKIETTLAAQGEQINKLTQTVEKQGEQINQLVQVVLLHGEQINKLTQTVEKQGEQIKELQIEQKAQGETLKLILQTLQKMSDRLDKMEVKMDKMEVKMDKMEVKMDKMEKRIDKLESK", "aa_hexdigest": "98cf4b48d6fb587165a7a107173fd118", "start_type": "GTG", "rbs_motif": "AACAA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01519.22", "name": "DUF16", "length": 54, "aa_cov": 0.38571428571428573, "hmm_cov": 1.0, "evalue": 4.624414866001141e-39, "score": 128.6022491455078, "start": 2, "stop": 55 } ], "seq_stats": { "molecular_weight": 16485.8895, "isoelectric_point": 6.6398401260375985 }, "id": "APKECPOBEN_1087", "locus": "GCA002090215_01083" }, { "type": "cds", "sequence": "contig_1", "start": 609246, "stop": 609503, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF12378.14" ], "nt": "GTGCGCGATAACATTGCCAAGGGCATCACTGCCGGTAGTAACACCCAACAAACCACGTATGATCCCACCCGAACCGAGGCCACCTTGACCACCGCCACCACCTTTGCGTTACGCCGGTATGACCTCGCCGGGCGCGCCTTATACGACCTCGATTTTTCGAAGTTAAACCCACAAACGCCCACGCGCGACCAAACCGGGCAGATCACCTTTAACCCCTTTGGCGGCTTTGGGCTGAGTGGGGCTGCACCCCAACAGTGA", "aa": "MRDNIAKGITAGSNTQQTTYDPTRTEATLTTATTFALRRYDLAGRALYDLDFSKLNPQTPTRDQTGQITFNPFGGFGLSGAAPQQ", "aa_hexdigest": "f069261336431c534981970471d38417", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF12378.14", "name": "P1_N", "length": 58, "aa_cov": 0.6823529411764706, "hmm_cov": 0.896551724137931, "evalue": 5.068996053965605e-09, "score": 31.335514068603516, "start": 27, "stop": 84 } ], "seq_stats": { "molecular_weight": 9232.065799999997, "isoelectric_point": 8.137860298156738 }, "id": "APKECPOBEN_1088", "locus": "GCA002090215_01084" }, { "type": "cds", "sequence": "contig_1", "start": 609534, "stop": 610043, "strand": "+", "frame": 3, "gene": null, "product": "putative protein MPN_502", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75571", "UniRef:UniRef90_P75285" ], "nt": "GTGGCCCAAGACCCCTCCAATCCTTATCGGTTTGCCGTTTTACTCGTGCCGCGTAGCGTGGTGTACTATGAGCAGTTGCAGCGGGGGTTAGCGCTCCCTAACCAAGGGAGTTCGTCAGGCTCCGGTCAACAAAACACCACCATTGGCGCGTATGGGCTGAAGGTGAAAAACGCCGAGGCGGACACCGCGAAGAGCAATGAAAAACTCCAGGGCTATGAATCCAAGTCTTCCAATGGATCTTCAAGCACTTCCACCACCCAACGTGGGGGTTCGTCAAATGAAAACAAAGTCAAGGCGTTGCAGGTGGCGGTGAAAAAGAAATCCGGGAGTCAGGGCAACTCCGGTGACCAAGGCACCGAACAGGTGGAACTTGAATCTAATGATTTAGCCAACGCCCCGATTAAACGAGGCTCCAATAACAACCAGCAAGTCCAACTCAAGGCGGACGATTTTGGTACTGCCCCTTCCAGTTCGGGATCAGGCACCCAAGATGGCACCCCCACCCCCTGA", "aa": "MAQDPSNPYRFAVLLVPRSVVYYEQLQRGLALPNQGSSSGSGQQNTTIGAYGLKVKNAEADTAKSNEKLQGYESKSSNGSSSTSTTQRGGSSNENKVKALQVAVKKKSGSQGNSGDQGTEQVELESNDLANAPIKRGSNNNQQVQLKADDFGTAPSSSGSGTQDGTPTP", "aa_hexdigest": "780379d1e8039b126587944a38c67f1a", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 609246, "stop": 610346, "observations": { "mutation": [ 609501, 610041, 610203, 610218, 610242 ], "stop": [ 609501, 610041, 610203, 610218, 610242 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75285", "query_cov": 1.0, "subject_cov": 0.4004739336492891, "identity": 0.9940000000000001, "score": 255.0, "evalue": 2.89e-81, "gene_start": 1, "gene_end": 169, "reference_start": 97, "reference_end": 265, "reference_sequence": "MRDNIAKGITAGSNTQQTTYDPTRTEATLTTATTFALRRYDLAGRALYDLDFSKLNPQTPTRDQTGQITFNPFGGFGLSGAAPQQWNEVKDKVPVEVAQDPSNPYRFAVLLVPRSVVYYEQLQRGLALPNQGSSSGSGQQNTTIGAYGLKVKNAEADTAKSNEKLQGYESKSSNGSSSTSTTQRGGSSNENKVKALQVAVKKKSGSQGNSGDQGTEQVELESNDLANAPIKRGSNNNQQVQLKADDFGTAPSSSGSGTQDGTPTPWTPWLTTEQIHNDPAKFAASILILYDAPYARNRTAIDRVDHLDPKVMTANYPPSWRTPKWNHHGLWDWKARDVLLQTTGFFNPRRHPEWFDGGQTVADNEKTGFDVDNSENTKQGFQKEADSDKSAPIALPFEAYFANIGNLTWFEQALLVFGICLS" }, "paralog": false, "identity": 0.9836065573770492, "score": 662.0, "evalue": 4.67e-244, "description": "Internal stop codon at 609501, 610041, 610203, 610218, 610242. Nonsense mutation around 609501, 610041, 610203, 610218, 610242.", "psc": { "uniref90_id": "UniRef90_P75285", "product": "Uncharacterized protein MPN_502", "uniref50_id": "UniRef50_P75571", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75285", "UniRef:UniRef50_P75571" ] }, "pscc": { "uniref50_id": "UniRef50_P75571", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75571" ], "product": "Uncharacterized protein MPN_205" } }, "id": "APKECPOBEN_1089", "locus": "GCA002090215_01085" }, { "type": "cds", "sequence": "contig_1", "start": 610260, "stop": 610514, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF12378.14" ], "nt": "TTGCTCCAAACCACCGGGTTCTTCAACCCGCGCCGCCACCCCGAGTGGTTTGATGGCGGGCAGACGGTCGCGGATAACGAAAAGACCGGGTTTGATGTGGATAACTCCGAAAACACCAAGCAGGGCTTTCAAAAGGAAGCTGACTCCGACAAGTCGGCCCCGATCGCCCTCCCGTTTGAAGCGTACTTCGCCAACATTGGCAACCTCACCTGGTTCGAGCAAGCGCTTTTGGTGTTTGGGATTTGTTTGTCTTAA", "aa": "MLQTTGFFNPRRHPEWFDGGQTVADNEKTGFDVDNSENTKQGFQKEADSDKSAPIALPFEAYFANIGNLTWFEQALLVFGICLS", "aa_hexdigest": "451778bce0a189a6b072b668f7a1372b", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF12378.14", "name": "P1_N", "length": 77, "aa_cov": 0.9166666666666666, "hmm_cov": 0.6623376623376623, "evalue": 1.9458650160195823e-17, "score": 58.95265197753906, "start": 4, "stop": 80 } ], "seq_stats": { "molecular_weight": 9421.290499999997, "isoelectric_point": 4.418060874938965 }, "id": "APKECPOBEN_1090", "locus": "GCA002090215_01086" }, { "type": "cds", "sequence": "contig_1", "start": 610659, "stop": 611123, "strand": "+", "frame": 3, "gene": null, "product": "Putative MgpC-like protein MPN_366", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75415", "UniRef:UniRef90_A0AB38W808" ], "nt": "GTGGAGGATACCAAGTTGGTGGCGCTGGTCCGTTCAGCGTTGGTCAGGGTGAAGTTTCAAGATAGTTCGAGCCAACAAGGCACTGAGAGTAATGGCCAAGACCAAAATGCCCTTAGTTTTGATACCACCAAGTCCCAACAAGCCTTACAGCAAGCTGGCGATCAATCAGGAATTTCATCTAATTCCACCCAAGAAGACTTCGCCAGCTACATCCTCATCTTTAAAGCCGCCCCGAAGGCCACGTGGGTGTTTGAACGGAAGATTAAGTTGGCGTTGCCCTACGTTAAGCAGGAAAGTCAGGGTTCCGACGATCAAGGTTCCAATGGTAAGGGCTCCCTCTACACCACCTTAAAAGACCTCCTCGTCGAACAACCCGTGACCCCTTACACGCCCAATGCGGGGTTAGCCCGGGTGAATGGGGTTGCTCAGGACAAGGTGCATGGGTTGGAACAAACCACTCAATGA", "aa": "MEDTKLVALVRSALVRVKFQDSSSQQGTESNGQDQNALSFDTTKSQQALQQAGDQSGISSNSTQEDFASYILIFKAAPKATWVFERKIKLALPYVKQESQGSDDQGSNGKGSLYTTLKDLLVEQPVTPYTPNAGLARVNGVAQDKVHGLEQTTQ", "aa_hexdigest": "0978fb526ff721c3fae22dcf45b54218", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 610608, "stop": 611294, "observations": { "insertion": [ 611238 ], "deletion": [ 611142 ], "mutation": [ 610650, 611121 ], "stop": [ 610650, 611121, 611229 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB38W808", "query_cov": 0.948051948051948, "subject_cov": 0.6160337552742616, "identity": 0.986, "score": 275.0, "evalue": 6.52e-92, "gene_start": 5, "gene_end": 150, "reference_start": 21, "reference_end": 166, "reference_sequence": "MRAAHLHTSYELVDWKVGQNKLVALVRSALVRVKFQDSSSQQGTESNGQDQNALSFDTTKSQQALQQAGDQSGTSSNSTQEDFASYILIFKAAPRATWVFERKIKLALPYVKQESQGSDDQGSNGKGSLYTTLKDLLVEQPVTPYTPNAGLARVNGVAQDKVHGLESGWDNQRSQKNLTNNPGPKAVTGFKLDKGRAYRKLNESWPVSNPSIRPRLARGKVPQNEKRRRQMLRVMLP" }, "paralog": false, "identity": 0.8596491228070176, "score": 361.0, "evalue": 4.43e-128, "description": "Internal stop codon at 610650, 611121, 611229. Frameshift due to insertion around 611238. Frameshift due to deletion around 611142. Nonsense mutation around 610650, 611121.", "psc": { "uniref90_id": "UniRef90_A0AB38W808", "uniref50_id": "UniRef50_P75415", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB38W808", "UniRef:UniRef50_P75415" ] }, "pscc": { "uniref50_id": "UniRef50_P75415", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75415" ], "product": "Putative MgpC-like protein MPN_366" } }, "id": "APKECPOBEN_1091", "locus": "GCA002090215_01087" }, { "type": "cds", "sequence": "contig_1", "start": 611120, "stop": 611230, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAACCAACAAGCCTCCCAAAAAACCTCACCAACAACCCCGCCCCCAAGGCCGTCACCGGCTTTAAGCTCGACAATGGCCGCGCGTACCGGAAGTTAAATGAAGCTTGA", "aa": "MKPTSLPKNLTNNPAPKAVTGFKLDNGRAYRKLNEA", "aa_hexdigest": "8dbba7ddc7163b721c7a87a4bfac4bcb", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3956.531000000001, "isoelectric_point": 10.288847923278805 }, "id": "APKECPOBEN_1092", "locus": "GCA002090215_01088" }, { "type": "cds", "sequence": "contig_1", "start": 611333, "stop": 611560, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAGCTCAACCGGGAGTCAAATGGCTGCAGTCACAGACAGTCAGCAATCAGGTCATAACAGTGGATTGGTATCGCTTGCCCAGCGCTCCACTACTGTTGCTGTCCAAAAAAGTGACTCTTCTGGTTTTCAAGGCCAAGGCACAACCGACAACAAATTCCAAAAATATTTAAACACCGCCCAGGCCTTGCACCAGATGGGGGTGATAGTGCCATCTTTGGAAACGTGA", "aa": "MSSTGSQMAAVTDSQQSGHNSGLVSLAQRSTTVAVQKSDSSGFQGQGTTDNKFQKYLNTAQALHQMGVIVPSLET", "aa_hexdigest": "77a80305a02f969b876e134cf9a2a425", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7832.513400000002, "isoelectric_point": 6.697645378112793 }, "id": "APKECPOBEN_1093", "locus": "GCA002090215_01089" }, { "type": "cds", "sequence": "contig_1", "start": 611819, "stop": 612376, "strand": "+", "frame": 2, "gene": "mgpC", "product": "MgpC family cytadherence protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002246AD21", "UniRef:UniRef90_UPI001FCE6DDF" ], "nt": "TTGAACTGGGGCACCGACAAGCAGAAGCAGTTTGTGGAGAATCAGCTTGGTTTTAAAGATGACTCAAATTCTTCCTTGACAAACTTCAAGAGTCAAGGCTTAACTCAGCCAGCTTACCTCATCGCCGGCCTTGACGTTGTCCAAGACCACCTCGTCTTTGCGGCCTTCAAAGCGGGCGCGGTGGGGTATGATATGACGACTGATTCGAACGCTTCCACCAAAGACCAAGCGCTCGCCTGGTCGACCACGGCCGGGTTGGACAGTGCTGGGGGGTACAACAACCTGGTGGAAAACACGGCCGGACTGAACGGCCCGATTAACGACTTGTTTACCCTGCTCGACACCTTTGCCTATGTGACCCCGGTGAGTGGGATGAAAGGGGGGAGTCAGAATAACGAAGAAGTGCAAACCAAGTATCCCGTTAAGGATGATAGTAAGGCGAGTGCAAAGATTGCCTCCTTAATTAATGCTTCGCCCTTAAACAGTTATGGGGATGATGGCGATAAAAAAGCCATCTACCCAGAGGTAAGAGGCAGACAGCTCCAAAATATTTTTTGA", "aa": "MNWGTDKQKQFVENQLGFKDDSNSSLTNFKSQGLTQPAYLIAGLDVVQDHLVFAAFKAGAVGYDMTTDSNASTKDQALAWSTTAGLDSAGGYNNLVENTAGLNGPINDLFTLLDTFAYVTPVSGMKGGSQNNEEVQTKYPVKDDSKASAKIASLINASPLNSYGDDGDKKAIYPEVRGRQLQNIF", "aa_hexdigest": "e3d23938cf56cea06e957840f36290b2", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001FCE6DDF", "query_cov": 1.0, "subject_cov": 0.8409090909090909, "identity": 0.9890000000000001, "score": 367.0, "evalue": 5.03e-128, "valid": true, "product": "MgpC family cytadherence protein", "uniref50_id": "UniRef50_UPI002246AD21" }, "pscc": { "uniref50_id": "UniRef50_UPI002246AD21", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002246AD21" ], "product": "MgpC family cytadherence protein" }, "genes": [ "mgpC" ], "id": "APKECPOBEN_1094", "locus": "GCA002090215_01090" }, { "type": "cds", "sequence": "contig_1", "start": 612782, "stop": 612892, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGAGAAAATTTCCGAAAAGGAATACAAAGCTTTAATCCGTAAAACTGGCAAAGAGCACTTTGATGGTGAAAAAGAAGAGTATGGTGATGGCACAGTTGGAGTTTGA", "aa": "MKEKISEKEYKALIRKTGKEHFDGEKEEYGDGTVGV", "aa_hexdigest": "435a21db0dde7c895a3c3aec2dc0ec06", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4130.589700000002, "isoelectric_point": 5.72024211883545 }, "id": "APKECPOBEN_1095", "locus": "GCA002090215_01091" }, { "type": "cds", "sequence": "contig_1", "start": 612929, "stop": 613162, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01519.22" ], "nt": "GTGAAAGTTAAATACGTAACCCAAGAACAATTTCAGGAATATAAAGATTCCAACAATCAGCGACTCATAAAGATTGAAAATAAGGTTGATAAATTAGTTGAAATTGTTCAAATTCACGGCGAACAAATTAAAGCACAGGGAGAAACCCTGCAATTAATTTTGCAAACGCTGCAAAAGATGAGTGATCGCTTGGACAAGATGGAAAAAAGAATAGACAAACTTGAGTCCAAATAA", "aa": "MKVKYVTQEQFQEYKDSNNQRLIKIENKVDKLVEIVQIHGEQIKAQGETLQLILQTLQKMSDRLDKMEKRIDKLESK", "aa_hexdigest": "21002b18daba759e88e7f408c2bc702c", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01519.22", "name": "DUF16", "length": 88, "aa_cov": 0.8701298701298701, "hmm_cov": 1.0, "evalue": 3.939212636606821e-20, "score": 67.77593231201172, "start": 2, "stop": 68 } ], "seq_stats": { "molecular_weight": 9203.579399999995, "isoelectric_point": 8.89162464141846 }, "id": "APKECPOBEN_1096", "locus": "GCA002090215_01092" }, { "type": "cds", "sequence": "contig_1", "start": 613386, "stop": 613628, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACATACCAAAGTTTTAAGGATTTGTCAATTGAAGCTAAGTTGAGCTATAACTTTAATTGGTTTGGAGATTATTCTTTAGGTGGTTTCACTGCTAAGAAAGGTGACAAGCATTATTTTGATCTTTTCCTTAAAATTAAATCTAATTCTGATCCAAAAAAACAATTTACTACAGAAAAATTTCTTACTAAAGAAGAAAAAAATAAAGTTAATGGAAAAGAAATTACAAGAAATTTAGAGTGA", "aa": "MTYQSFKDLSIEAKLSYNFNWFGDYSLGGFTAKKGDKHYFDLFLKIKSNSDPKKQFTTEKFLTKEEKNKVNGKEITRNLE", "aa_hexdigest": "39c150ec8bb2bbb8defe527cce6713ed", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9428.558299999997, "isoelectric_point": 9.301643562316894 }, "id": "APKECPOBEN_1097", "locus": "GCA002090215_01093" }, { "type": "cds", "sequence": "contig_1", "start": 613743, "stop": 613991, "strand": "-", "frame": 2, "gene": null, "product": "putative lipoprotein MPN_467", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P75316", "UniRef:UniRef90_P75316" ], "nt": "ATGCGCAAAAAGAGATTGTTGTCAAGGATTTCCTTTAGTTCTCTATTTTTATTATGTGGAACAGTTTTAAGCGCCTATACTGGCATCCAAGCAGATCTCAGAAATCTTATCAAGGAAACCACCAAAAAAGACATTGATGTTTACAAGGCAATTAAAACTACAGAAGGAAAAAAGAACATAATAACTTCTTTAAAGAAATCTTACGAAGTTAATCCGAAAGATACAACAAAATTGTTGTTAGACGCCTGA", "aa": "MRKKRLLSRISFSSLFLLCGTVLSAYTGIQADLRNLIKETTKKDIDVYKAIKTTEGKKNIITSLKKSYEVNPKDTTKLLLDA", "aa_hexdigest": "ed29bf6947df9f89923155db9a0c3d5a", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75316", "query_cov": 1.0, "subject_cov": 0.8118811881188119, "identity": 0.902, "score": 137.0, "evalue": 1.98e-40, "valid": true, "product": "Uncharacterized lipoprotein MPN_467", "uniref50_id": "UniRef50_P75316", "go_ids": [ "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75316", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75316" ], "product": "Uncharacterized lipoprotein MPN_467" }, "genes": [], "id": "APKECPOBEN_1098", "locus": "GCA002090215_01094" }, { "type": "cds", "sequence": "contig_1", "start": 614419, "stop": 614625, "strand": "+", "frame": 1, "gene": null, "product": "Family K-like protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD640D", "UniRef:UniRef100_A0AAP8E4R5", "UniRef:UniRef50_A0AAP8E4D4", "UniRef:UniRef90_A0AAP8E4D4" ], "nt": "ATGATAGAAGTATTAAGTGATTCTGAAATTTTGTCTTATTTATTAAGTAATAAGCACACTGCTGATGGAGTTAATGAAATTTATGTAACGATGAACATTAAAGACAAAGATTCAAACACTCAAAGGAAGCCAGATAAAATGTCTTTAGAGCTTGAATTGTTTTTAAATCAGTTTATTGAATTCTTAAAAGAGCAGAGAAAAAGCTAG", "aa": "MIEVLSDSEILSYLLSNKHTADGVNEIYVTMNIKDKDSNTQRKPDKMSLELELFLNQFIEFLKEQRKS", "aa_hexdigest": "71d7b59af7957e717843d8a92e09190f", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD640D", "uniref100_id": "UniRef100_A0AAP8E4R5", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD640D", "UniRef:UniRef100_A0AAP8E4R5" ] }, "ips": { "uniref100_id": "UniRef100_A0AAP8E4R5", "uniref90_id": "UniRef90_A0AAP8E4D4" }, "psc": { "uniref90_id": "UniRef90_A0AAP8E4D4", "product": "Family K-like protein", "uniref50_id": "UniRef50_A0AAP8E4D4" }, "pscc": { "uniref50_id": "UniRef50_A0AAP8E4D4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAP8E4D4" ], "product": "Family K-like protein" }, "genes": [], "id": "APKECPOBEN_1099", "locus": "GCA002090215_01095" }, { "type": "cds", "sequence": "contig_1", "start": 614907, "stop": 615710, "strand": "+", "frame": 3, "gene": null, "product": "putative protein MPN_146", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75140" ], "nt": "ATGAAATTTAAGTATGGTGCCATCTTTTTTAGTGGCTTTCTAGGTCTTTCTGCCATTTTGGCTGCCTGTGGTACAAAGGGTAAATTTGATCAAGTTGATGATGGCAAGATTAAATTGGCTTCCTCTTTAACTTCTAAAAGTGCATCAAAAGCCTTACAAGCAATTGTTAAAAAATATAACGAAGTTAAAAAACCTGGTGATTACCCTATTGAAATTACCCAAATCGCTGGTGGTTATGATGGTGGTCGTAGCGATTTACAAACCCGAGTCAACGTTAAAGACACCACTAACTTTTACAACTTAATCTTAAACTACCCTGATCTAGTTTCAACTTTAGGTCGTGTTGGTATGGAACTGCCGTTTGACAATGTAAAGGTTGACAAACTATCACCCCGTTTTTTAGATTTCAACAACCGCATTAGTGCAATTTCTAAACCAGGAATTTACGGTATTCCGGTTTCTTTATCCACCGAAGTTTTATCGATCAACGGACCGGTGTTGCACTATATTTTGAACAACGCTAAAAAGAAAGAAGGTACTTTAAACCAAAAAATGACAAGTTCTTCTGAAGGAAAAAACAGCAGTGGCACTTTAACAGTAGCAACTGATACTGAAACAAGTAGTTTATGGAAAAAGATAGAAGATTCTGCAAAAGCTAACGGTAAAAGCGATGAAAAAGGAAAAGGTAAGAAGAAAGATAATAAAAGTGCAACTTTTTCGCTTGTACAACTAAAACAAACTCAAGAAAAAACAGATGATTCCCAAGACACTAAAAATAGTGATGATCAAGTTAAAAAATCTTGA", "aa": "MKFKYGAIFFSGFLGLSAILAACGTKGKFDQVDDGKIKLASSLTSKSASKALQAIVKKYNEVKKPGDYPIEITQIAGGYDGGRSDLQTRVNVKDTTNFYNLILNYPDLVSTLGRVGMELPFDNVKVDKLSPRFLDFNNRISAISKPGIYGIPVSLSTEVLSINGPVLHYILNNAKKKEGTLNQKMTSSSEGKNSSGTLTVATDTETSSLWKKIEDSAKANGKSDEKGKGKKKDNKSATFSLVQLKQTQEKTDDSQDTKNSDDQVKKS", "aa_hexdigest": "3e99355df59412f73486d842bc3f3442", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75140", "query_cov": 1.0, "subject_cov": 0.9434628975265018, "identity": 0.539, "score": 242.0, "evalue": 2.49e-76, "valid": false, "product": "Uncharacterized protein MPN_146", "uniref50_id": "UniRef50_P75140" }, "pscc": { "uniref50_id": "UniRef50_P75140", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75140" ], "product": "Uncharacterized protein MPN_146" }, "genes": [], "id": "APKECPOBEN_1100", "locus": "GCA002090215_01096" }, { "type": "cds", "sequence": "contig_1", "start": 615900, "stop": 616904, "strand": "+", "frame": 3, "gene": null, "product": "P68 family surface lipoprotein", "db_xrefs": [ "GO:0005886", "GO:0009897", "SO:0001217", "UniRef:UniRef50_P75280", "UniRef:UniRef90_P75280" ], "nt": "TTGTTTACCTCTGTTTTTGCAGCTGGCGGCGGTGATTACAATAATTTCTTTTACAAGATTGAAAATGGTCGCGCTGACTTTAGTAACTTTAAGAATAAAGGGACTTCTTACCAAAATCTTCAAAAAGTATTTGGTGATTTCAAAGGCTTAATTGACAAAAATGGTATCTTTGTGAATAAGGGTGGATCTTACTCATCAAACTTCCAAAAGTTCCACCAATTAGCTTACAGCATTTCCTCTACTTCCGGTTTCTTCTATTCGTTTGCCGGTAAAAGTGCAAAGCGTTTAAATTTTGGTGATAGCTTTATTGAGTATCCGCGGTTTACTCAAGAAATTAAAGCACCATCAAAAAATGGAGAAAATGGCCAAACAAATGAAGGAAATAGTACCAATGGCGAACAAAATCTCTTAGGAACTTTCGAAGTCAAGGATGACAGTAAGCCTAAAGAAGAAGTTAAAAGTAATAAAAATAGTGGTAAAGAAAGTAGCCAAAATCAGGGTAAAAAATCTAACAATAACAAGACTATTTACCTTTATGAAACAAAAATTCCAGATGGAAAAACTGCAGGTGATAATGCTATCTTAATCAAAGATAAGAACGTTATCGAAAAGCTTAAAAGTGCAGCTAAAGAAGAAAATAAAGAACAAACTGCTGAAGCAACTAAAGCTGCAATTACAAGTAATAAAGCAAAAAGCACTAAAAAAGAAAGCAGTAAAGTTATCGGTTACACCACAACTGATAGTGTTCGCGAAGATGGCAAAAACATTTTCGCAATAGATAGAGTTAATGGTGAAAACTATGACCGTAAGATTATTGTAGGTGCTAAAGCAGAAACTTTAAACCAATCTAGCACCTTACAAAGTGAAGAAGCGATTGTTTTGCCTGCCCCTGGAAAGTATCTAAATGGTGATCCCAAGAAAGTGACAATTACCCAAGGACCTAACATCATTGGCATTCATGCTAACGAAAAGGAAAATGCAGAAACGCAAAAGTTCGTAGATTGA", "aa": "MFTSVFAAGGGDYNNFFYKIENGRADFSNFKNKGTSYQNLQKVFGDFKGLIDKNGIFVNKGGSYSSNFQKFHQLAYSISSTSGFFYSFAGKSAKRLNFGDSFIEYPRFTQEIKAPSKNGENGQTNEGNSTNGEQNLLGTFEVKDDSKPKEEVKSNKNSGKESSQNQGKKSNNNKTIYLYETKIPDGKTAGDNAILIKDKNVIEKLKSAAKEENKEQTAEATKAAITSNKAKSTKKESSKVIGYTTTDSVREDGKNIFAIDRVNGENYDRKIIVGAKAETLNQSSTLQSEEAIVLPAPGKYLNGDPKKVTITQGPNIIGIHANEKENAETQKFVD", "aa_hexdigest": "f7e6ece2e523049b72374748f2386385", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 615600, "stop": 617207, "observations": { "mutation": [ 615708, 615777, 616902, 616926 ], "stop": [ 615708, 615777, 616902, 616926 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75280", "query_cov": 1.0, "subject_cov": 0.41802252816020025, "identity": 0.997, "score": 617.0, "evalue": 2.52e-215, "gene_start": 1, "gene_end": 334, "reference_start": 338, "reference_end": 671, "reference_sequence": "MKFKYCAIFFSGFLGLSAILAACGARGKFDQVDDGKIKLAFSLTSKSASNALQAIVKKYNEVKKPGDYHIEITQIAGGYDGGRSDLQTRVNVKDTTNFYNLILNYPDLVSTLGRVGMELLFDKVNTDKLSDRFLDFNKRISAISKPGIYGIPVSLSTEVLSINGPVLHYILKSAKGDSDNVKVSQRTGETTQKSKVTNPLKINTQNDPATKDLWEKIEASAKANGKSNKDGQTKGKKKVEKSSSSSLVNLKQSTDQTTTDDGSQKSDNKIKESWGEYQEVDGGLKNFEFKASIFENWHDLLDFSTRVAKSFKKIHENSNKKGNDIQGILGVDSTPNSLFTSVFAAGGGDYNNFFYKIENGRADFSNFKNKGTSYQNLQKVFGDFKGLIDKNGIFVNKGGSYSSNFQKFHQLAYSISSTSGFFYSFAGKSAKRLNFGDSFIEYPRFTQEIKAPSKNGENGQTNEGNSTNGEQNLLGTFEVKDDSKPKEEVKSNKNSGKESSQNQGKKSNNNKTIYLYETKIPDGKTAGDNAILIKDKNVIEKLKSAAKEENKEQTAEATKAAITSNKAKSTKKESSKVIGYTTTDSVREDGKNIFAIDRVNGENYDRKIIVGAKAETLNQSSTLQSEEAIVLPAPGKYLNGDPKKVTITQGPNIIGIHANEKENAETQKFVDWFLNSPQTWEKQSRDKKGSSEKQTAAEFFAESASYILPLKEIFDKNDTKTEKGKNSKTQQRTNTYAEKALELFKQISQNQIVSYSDPSDFRSGKFRDAIGATFNAAVSSKADFNKFVQNFTATLGSDI" }, "paralog": false, "identity": 0.9495327102803738, "score": 981.0, "evalue": 0.0, "description": "Internal stop codon at 615708, 615777, 616902, 616926. Nonsense mutation around 615708, 615777, 616902, 616926.", "psc": { "uniref90_id": "UniRef90_P75280", "product": "P68 family surface lipoprotein", "uniref50_id": "UniRef50_P75280", "go_ids": [ "GO:0005886", "GO:0009897" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75280", "UniRef:UniRef50_P75280", "GO:0005886", "GO:0009897" ] }, "pscc": { "uniref50_id": "UniRef50_P75280", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75280" ], "product": "Uncharacterized lipoprotein MPN_506" } }, "id": "APKECPOBEN_1101", "locus": "GCA002090215_01097" }, { "type": "cds", "sequence": "contig_1", "start": 617109, "stop": 617288, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000A45C19C" ], "nt": "TTGGAACTCTTCAAACAAATTAGTCAAAATCAAATAGTAAGCTACAGTGATCCAAGTGACTTTAGGAGTGGAAAATTCCGTGATGCAATTGGTGCTACTTTCAATGCTGCAGTTAGTTCCAAAGCAGACTTCAATAAATTCGTCCAAAACTTTACCGCTACATTAGGTAGTGACATTTAA", "aa": "MELFKQISQNQIVSYSDPSDFRSGKFRDAIGATFNAAVSSKADFNKFVQNFTATLGSDI", "aa_hexdigest": "5c0aacd6d3654e50d73802bf158724bc", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI000A45C19C", "query_cov": 0.9152542372881356, "subject_cov": 0.9473684210526315, "identity": 0.815, "score": 92.4, "evalue": 1.1e-23, "valid": false, "product": "Putative mycoplasma lipoprotein, C-terminal region", "uniref50_id": "UniRef50_UPI000A45C19C" }, "pscc": { "uniref50_id": "UniRef50_UPI000A45C19C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000A45C19C" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6512.145100000002, "isoelectric_point": 5.987158012390137 }, "id": "APKECPOBEN_1102", "locus": "GCA002090215_01098" }, { "type": "cds", "sequence": "contig_1", "start": 617408, "stop": 617587, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01420.25" ], "nt": "GTGCAAATTAGGACTTACAAAATCAAAGATATTTGCGACATTCAACGTGGTCGTGGTATCACTAAGGAATACATTAAAAATAACTCTGGAAAATATCCTGTTTATTCTGCCGCTACTACCAATAACGGTGAGTTAGGTTTTATTAATACTTACGATTTTGCTGGTGAATATGTTACCTGA", "aa": "MQIRTYKIKDICDIQRGRGITKEYIKNNSGKYPVYSAATTNNGELGFINTYDFAGEYVT", "aa_hexdigest": "c88d0c6f6d89cfd8664377c21c3e12f3", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01420.25", "name": "Methylase_S", "length": 59, "aa_cov": 0.9322033898305084, "hmm_cov": 0.9830508474576272, "evalue": 3.5293963533972237e-12, "score": 42.29743957519531, "start": 5, "stop": 59 } ], "seq_stats": { "molecular_weight": 6731.4927000000025, "isoelectric_point": 8.786992454528807 }, "id": "APKECPOBEN_1103", "locus": "GCA002090215_01099" }, { "type": "cds", "sequence": "contig_1", "start": 617771, "stop": 618409, "strand": "+", "frame": 2, "gene": "hsdS", "product": "Putative type I specificity subunit S-MpnORF507P", "db_xrefs": [ "COG:COG0732", "COG:V", "EC:3.1.21.3", "GO:0003677", "GO:0009307", "KEGG:K01154", "SO:0001217", "UniRef:UniRef50_P75279", "UniRef:UniRef90_P75279" ], "nt": "GTGGTAGCTGAAATCTCCTTAGATTTTCCACCTCTAGAAGTTCAAGAAAAAATTGCTCATTTTCTCAAATCTTTTAATGAATTAAGCAGCCAGTTAAAAGCTGAATTAATTAAACGACAAAAGCAATACGCATTTTACAGTGATTATCTACTCAATCCTAAACATTCACAAGGTGAAGAATACAAACTCTTTAAACTCAAAGATATAGCTAAAAAGATTTTAGTTGGCGGTGAAAAGCCAAGTGATTTCCAAAAAGAAAAGGATCAAGTTTATAAATACCCTATTCTTTCTAATAGTCGCAAAGCAGACGATTTTTTAGGCTACAGCAAAACGTTTAGAATTGCTGAAAAATCAATTACAGTTAGTGCAAGAGGCACAATTGGTGCTGTTTTTTACCGTGATTTTTCCTACTTACCCGCTGTTAGTCTAATTTGCTTTATTCCTAAGCCAGAATTTAATATTAATTTTTTGTTTCATGCTTTAAAAGCGACTAAATTCCACAAACAAGGTTCTGGCACAGGACAGTTAACCATGGCACAGTTTAAAGAATATCAGGTTTATATTCCATCTTTAAAAAAACAACAAGAAATTGCTGCCACTTTGGATCCACTTTACTATATCTTTGCAAATTCTAATTGA", "aa": "MVAEISLDFPPLEVQEKIAHFLKSFNELSSQLKAELIKRQKQYAFYSDYLLNPKHSQGEEYKLFKLKDIAKKILVGGEKPSDFQKEKDQVYKYPILSNSRKADDFLGYSKTFRIAEKSITVSARGTIGAVFYRDFSYLPAVSLICFIPKPEFNINFLFHALKATKFHKQGSGTGQLTMAQFKEYQVYIPSLKKQQEIAATLDPLYYIFANSN", "aa_hexdigest": "60ad333f7270d865d0cc0fc5e742a999", "start_type": "GTG", "rbs_motif": null, "genes": [ "hsdS" ], "truncated": "both", "pseudogene": { "start": 617471, "stop": 618499, "observations": { "mutation": [ 617585, 618407, 618476 ], "stop": [ 617585, 618407, 618476 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75279", "query_cov": 1.0, "subject_cov": 0.5840220385674931, "identity": 0.995, "score": 414.0, "evalue": 6.04e-144, "gene_start": 1, "gene_end": 212, "reference_start": 122, "reference_end": 333, "reference_sequence": "MQIRTYKIKDICDIQRGRGITKEYIKNNSGKYPVYSAATTNNGELGFINTYDFAGEYVTWTTNGYAGVVFYRNGKFSASQDCGVLKVRNKEINAQFLAFALSLKTPQFVHNLGSRPKLNRKVVAEISLDFPPLEVQEKIAHFLKSFNELSSQLKAELIKRQKQYAFYSDYLLNPKHSQGEEYKLFKLKDIAKKILVGGEKPSDFQKEKDQVYKYPILSNSRKADDFLGYSKTFRIAEKSITVSARGTIGAVFYRDFSYLPAVSLICFIPKPEFNINFLFHALKATKFHKQGSGTGQLTMAQFKEYQVYIPSLKKQQEIAATLDPLYYIFANSNWGIYKEIELRKKQMQYYQERLFQWIENQKV" }, "paralog": false, "identity": 0.9912280701754386, "score": 673.0, "evalue": 1.56e-248, "description": "Internal stop codon at 617585, 618407, 618476. Nonsense mutation around 617585, 618407, 618476.", "psc": { "uniref90_id": "UniRef90_P75279", "gene": "HsdS", "product": "Putative type I specificity subunit S.MpnORF507P", "ec_ids": [ "3.1.21.3" ], "uniref50_id": "UniRef50_P75279", "cog_id": "COG0732", "cog_category": "V", "kegg_orthology_id": "K01154", "go_ids": [ "GO:0003677", "GO:0009307" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75279", "EC:3.1.21.3", "UniRef:UniRef50_P75279", "COG:COG0732", "COG:V", "KEGG:K01154", "GO:0003677", "GO:0009307" ] }, "pscc": { "uniref50_id": "UniRef50_P75279", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75279" ], "product": "Putative type I specificity subunit S.MpnORF507P" } }, "id": "APKECPOBEN_1104", "locus": "GCA002090215_01100" }, { "type": "cds", "sequence": "contig_1", "start": 618527, "stop": 618793, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03072.19" ], "nt": "ATGAACTTAAAGTTCATTGTTGATGGATCAATTCCAGTTTACACCGTTGCTAAAAAAGACTATCCTGGTTCTCTCTTCCAGTTTAATGACAAGGATGAATTGTTATTTACATTGTATGTCAAAGAACAGATTTCAGTTGCCGATCCAAACTTTATGAATTTATTGAGAGGACAAAACTTGCATGATTTAGAACTAGTCACCGGAGCTACCAAACCACCGGTAGTTGATCTAGCCAGCTATTTACACTTTGTTCTTTTAAGCGCTTAA", "aa": "MNLKFIVDGSIPVYTVAKKDYPGSLFQFNDKDELLFTLYVKEQISVADPNFMNLLRGQNLHDLELVTGATKPPVVDLASYLHFVLLSA", "aa_hexdigest": "9f2c3dc13ccd8653fd0eea10ed413ed9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03072.19", "name": "DUF237", "length": 48, "aa_cov": 0.5454545454545454, "hmm_cov": 1.0, "evalue": 5.974427293864996e-18, "score": 60.71588134765625, "start": 1, "stop": 48 } ], "seq_stats": { "molecular_weight": 9888.326399999993, "isoelectric_point": 5.066252708435058 }, "id": "APKECPOBEN_1105", "locus": "GCA002090215_01101" }, { "type": "cds", "sequence": "contig_1", "start": 619037, "stop": 619210, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAAGCAGATAGAAACAGAACTTTCAGTTTCTTAATTGCTGGATTTCGAACTGCCATTGATTGGTATTACAACGCTAAAAAAGAAAATAACGATGCGAAGCAAAAGGCCTTTGGTAGTCAAGGAATCCAATTCCCTAAAGATGGTTTAAATGGAATTTACATGTCCGATTGA", "aa": "MEADRNRTFSFLIAGFRTAIDWYYNAKKENNDAKQKAFGSQGIQFPKDGLNGIYMSD", "aa_hexdigest": "dfe380c494a1cd9798e2d5462de4b0aa", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6541.214400000003, "isoelectric_point": 8.034259605407716 }, "id": "APKECPOBEN_1106", "locus": "GCA002090215_01102" }, { "type": "cds", "sequence": "contig_1", "start": 619271, "stop": 619618, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03086.20" ], "nt": "GTGCATAAGCTAATTTCAAACGGCACAAATGATTTAACTACACTTGCTCAAACTGCTTTAGGTGATTCTTTATTTGATACTAAAGTTAACCTGACAGAATCAGTTATTGAAATAAATAACCAAACAGATGTTGCAACTAAATTTAGGGAAAAAGTACTCAATTCTTTCAAGCAAGAAAGAGAAAAAGCACATGCTGAGCACGTTGAAAAGTTAAGGAAACTAGAAGAGGAGCGTAAACTTCAAGAAGCAGAGGCCAAAGCAAAAGCAGAGGAAGTTAAAAAACTCGAAGCAGAAAGAGAAGCTTTTAACAAATCTCTAACAGCTGCATCAGAATTCAAACAATATTGA", "aa": "MHKLISNGTNDLTTLAQTALGDSLFDTKVNLTESVIEINNQTDVATKFREKVLNSFKQEREKAHAEHVEKLRKLEEERKLQEAEAKAKAEEVKKLEAEREAFNKSLTAASEFKQY", "aa_hexdigest": "072de0bffe78ae5dcf50fafceb900a01", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03086.20", "name": "DUF240", "length": 35, "aa_cov": 0.30434782608695654, "hmm_cov": 0.9714285714285714, "evalue": 3.987422252183696e-07, "score": 25.069747924804688, "start": 2, "stop": 36 } ], "seq_stats": { "molecular_weight": 13149.614399999988, "isoelectric_point": 5.972948265075684 }, "id": "APKECPOBEN_1107", "locus": "GCA002090215_01103" }, { "type": "cds", "sequence": "contig_1", "start": 619664, "stop": 620056, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03086.20" ], "nt": "ATGAATCAACTTCAAGCAGAAATAAATGCAGCTAATGCTGTGTTTCCTGTTTCCGACAGCACTAAAATTCCTAAAGTGTCACAAAAGCTTTTTGGTTTACTTGGTGATGGTTTCTTTCCGCAATTACATCCAAAGGGTTTAAAGATTGCTGACAATATTGCTGCATTATTCGATCAATATAACCTTAAGTCGATTGCACTCAAAAACTTTGATTTAAATTTAGAGCGAAAAAACGACATAGTCATTCAGGGCAAAGTTTGTTATAGTTTTTCCATCAAAATGGATTTTGCAACTATTTATGAAGGTGATGGTAGCACGATAGATTTACAGTTTGCACTCAACGCATCCACTACTAATTTTGCCAATCTAACAGATTTACAAGACAGTTTCTGA", "aa": "MNQLQAEINAANAVFPVSDSTKIPKVSQKLFGLLGDGFFPQLHPKGLKIADNIAALFDQYNLKSIALKNFDLNLERKNDIVIQGKVCYSFSIKMDFATIYEGDGSTIDLQFALNASTTNFANLTDLQDSF", "aa_hexdigest": "cac3197d6f4b36faea8ace113ed0891f", "start_type": "ATG", "rbs_motif": "AAAA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03086.20", "name": "DUF240", "length": 134, "aa_cov": 1.0, "hmm_cov": 1.0, "evalue": 2.4232931929862834e-43, "score": 143.6189727783203, "start": 1, "stop": 130 } ], "seq_stats": { "molecular_weight": 14356.163999999977, "isoelectric_point": 4.924496269226074 }, "id": "APKECPOBEN_1108", "locus": "GCA002090215_01104" }, { "type": "cds", "sequence": "contig_1", "start": 620186, "stop": 620395, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTGGCTTTTTGTACCACTACTGTAACTTTAGGCGCATTTTTATTTCCTACATTAACTCAAAAAGGTTCTAGTGCTGACATTAATACTTTAGCACTACACAATAAAAGTTTAGCTAGAGCCCATAATGACAGCTACTTGGTCAATCTTGCTAACGCATCTACTTTTAGTGAGGAAGAGTTAGCTGTAATAGCGAAAAATAAGGATTGA", "aa": "MLAFCTTTVTLGAFLFPTLTQKGSSADINTLALHNKSLARAHNDSYLVNLANASTFSEEELAVIAKNKD", "aa_hexdigest": "f238f6424a5db8fb642efc85b7fc46d7", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7401.301099999999, "isoelectric_point": 6.020522499084472 }, "id": "APKECPOBEN_1109", "locus": "GCA002090215_01105" }, { "type": "cds", "sequence": "contig_1", "start": 621739, "stop": 622002, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03086.20" ], "nt": "TTGTTTAATGACTACAACTTAAATTCAATTAAGTTAAAAGCGTTTAACTTGCAGTTGCTCAAACAAAACGACATTGTTTTAAAGAACAAGGTGCGCTATAGTTTTGCCATCGAAATGAAATTTCACACCGTTTATGTTGGCAAAAATACCAAAATTGACCTTGATTTTGCTCTGCAAGCTCAAACGAATAACTTTAGCTCTTTAGAGGAATTACGTGAAAGTTTTACTAATAGTGGTCAAACACTCTCAACCCAATTGTTCTGA", "aa": "MFNDYNLNSIKLKAFNLQLLKQNDIVLKNKVRYSFAIEMKFHTVYVGKNTKIDLDFALQAQTNNFSSLEELRESFTNSGQTLSTQLF", "aa_hexdigest": "252eb302644e232664999629963a48f0", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03086.20", "name": "DUF240", "length": 87, "aa_cov": 1.0, "hmm_cov": 1.0, "evalue": 1.9437065417270944e-29, "score": 98.10533142089844, "start": 1, "stop": 87 } ], "seq_stats": { "molecular_weight": 10130.437499999993, "isoelectric_point": 9.004250907897951 }, "id": "APKECPOBEN_1110", "locus": "GCA002090215_01106" }, { "type": "cds", "sequence": "contig_1", "start": 622918, "stop": 623109, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03072.19" ], "nt": "TTGGCTAACTGGGGTCTTGGTGGCATTCCGCCAGAATGGAGTGGTGACATGGAATTGAAGTTCCAAATTGATGGTAAGCTAGCTAACTGGATTACCCAAAAGCAAGATTACCCTGGTTCGTTATTCCAATTTCAAAATGACAAACTGTTGTTTACCCTACACGTAGTGCAACGTATTACCGTTAAGGATTAA", "aa": "MANWGLGGIPPEWSGDMELKFQIDGKLANWITQKQDYPGSLFQFQNDKLLFTLHVVQRITVKD", "aa_hexdigest": "50caf7dbb2e2ed5384acb7ddd6eafefb", "start_type": "TTG", "rbs_motif": "AAAA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03072.19", "name": "DUF237", "length": 59, "aa_cov": 0.9206349206349206, "hmm_cov": 1.0, "evalue": 1.0658609825245086e-27, "score": 92.28820037841797, "start": 6, "stop": 63 } ], "seq_stats": { "molecular_weight": 7265.242, "isoelectric_point": 5.613896369934082 }, "id": "APKECPOBEN_1111", "locus": "GCA002090215_01107" }, { "type": "cds", "sequence": "contig_1", "start": 623617, "stop": 623919, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03086.20" ], "nt": "GTGGTAGAACAATTAACCACAGATGGTGGCAATGATCTTACCAACATTGCTAAAACAGCAATTGGTGAAAGTTTGTTTAACCTCAAGGTTAACTTAACTGATTCAATAATTGATCAAAGCGTTTTACAGCAAGCGCAAAAATCATTTGAGGACAAGATCTTAGATCCGTTCCACGCTGAACGAGTTGAAGCTAAGCGCATTCATGATGAAGAAGCAAGACGCTTAGAGGCGGAACGAAAACGGATTGCAGCTGAATTGAAAGCCAAGGAAGATGAAGCTCGTCGTATTCGTGAAGAACATTGA", "aa": "MVEQLTTDGGNDLTNIAKTAIGESLFNLKVNLTDSIIDQSVLQQAQKSFEDKILDPFHAERVEAKRIHDEEARRLEAERKRIAAELKAKEDEARRIREEH", "aa_hexdigest": "b54a6cae6e0e8032f9d0c95e6e56fd6f", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03086.20", "name": "DUF240", "length": 36, "aa_cov": 0.36, "hmm_cov": 0.9722222222222222, "evalue": 2.9096157955859755e-08, "score": 28.79109764099121, "start": 2, "stop": 37 } ], "seq_stats": { "molecular_weight": 11482.709799999988, "isoelectric_point": 5.236599159240721 }, "id": "APKECPOBEN_1112", "locus": "GCA002090215_01108" }, { "type": "cds", "sequence": "contig_1", "start": 623926, "stop": 624294, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03086.20" ], "nt": "ATGCTTACTGTCCCTAAAGTGCCGGAAAACTTCTTTAGTTTCTTTCAGGAAGATTACTTTCCGCGGTTAACTCCCCGTGGTTTGAACATTGCCGATAATGTGGCTTCCTTGTTTAATGACTACAACTTAAATTCAATTGACTTTACTGGTTTTAACCTAAAACTGTTGCGCCAAAACGACATTGTTTTAAAGAATAAGGTGCGTTACAACTTCGCTCTTCAAATGGGCTTTGACACGGTTTACGCTGGAAAGAACACCACTATTTTAAAGTTTAGTTTACAAGCCCAAACAATAAACTTCACTAGCTTACAAGAACTACGTGATAGTTTTGAAAACAATGGCAACACTCTTTCAACCCATCTATTCTGA", "aa": "MLTVPKVPENFFSFFQEDYFPRLTPRGLNIADNVASLFNDYNLNSIDFTGFNLKLLRQNDIVLKNKVRYNFALQMGFDTVYAGKNTTILKFSLQAQTINFTSLQELRDSFENNGNTLSTHLF", "aa_hexdigest": "ba99fc209b20e23f6fffaba29767945e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03086.20", "name": "DUF240", "length": 120, "aa_cov": 0.9754098360655737, "hmm_cov": 1.0, "evalue": 3.247248539937413e-36, "score": 120.28932189941406, "start": 4, "stop": 122 } ], "seq_stats": { "molecular_weight": 14103.79939999998, "isoelectric_point": 6.551228141784668 }, "id": "APKECPOBEN_1113", "locus": "GCA002090215_01109" }, { "type": "cds", "sequence": "contig_1", "start": 624906, "stop": 625046, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCAAGAGCCAGAATTCATGGAATTAATTAAGTCACAAAACCTCCATAACTTAGAACTACGTACCGAGTCCACAAAGCCGCCAGTAGTGGATTTAGCGAGTTATTTACACTACCAACTGTTGAAGTTAAATCAACAGTAA", "aa": "MQEPEFMELIKSQNLHNLELRTESTKPPVVDLASYLHYQLLKLNQQ", "aa_hexdigest": "7f344c8e8368acec30a9529943802a50", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5468.2211, "isoelectric_point": 5.461397361755371 }, "id": "APKECPOBEN_1114", "locus": "GCA002090215_01110" }, { "type": "cds", "sequence": "contig_1", "start": 625239, "stop": 625688, "strand": "-", "frame": 2, "gene": null, "product": "putative protein MPN_511", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75275", "UniRef:UniRef90_P75275" ], "nt": "GTGCAAGCCTTTAAAGAATACTGGCAAAAGCAAAAGAAAGATGTTACCGATAAAAAGCAGCTGTTAGAAGCTTTAAAACTTTCCTTTGCCAAAGAACAAAATAAGACCTTTGCCTTTTTGATTAAGAACTTCCAGGACGGTATTAGCAATTATTACCCAAATGACCAAGAAGATCAAAGTGAGGCTGCTAAAACTGCTTTTGGTACACAAGGTATCGCCTTTCCCCAATCTGGTTTAAAGGGCATCTTTATGTCGGAATGGTTGCGTAAACAGCTAGGGGAAAAGGCCAAGATTAACTTAGACATAAAGAGCTTAAAGGTAACTGATTCAAAGATAAGTCCTACCATTAAATGGAACAAAGATATCGGTATTAAGCGAAATCAAGACAAACCTTACAACTTCCGCTTTGAGATTGACATTGAATATCAAGGTAATTACAAATTAAGTTGA", "aa": "MQAFKEYWQKQKKDVTDKKQLLEALKLSFAKEQNKTFAFLIKNFQDGISNYYPNDQEDQSEAAKTAFGTQGIAFPQSGLKGIFMSEWLRKQLGEKAKINLDIKSLKVTDSKISPTIKWNKDIGIKRNQDKPYNFRFEIDIEYQGNYKLS", "aa_hexdigest": "551226bacf23f303f751412bc9ba648a", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 624936, "stop": 625688, "observations": { "mutation": [ 625061, 625148, 625196, 625241 ], "stop": [ 625061, 625148, 625196, 625241 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75275", "query_cov": 1.0, "subject_cov": 0.573076923076923, "identity": 1.0, "score": 297.0, "evalue": 2.44e-100, "gene_start": 1, "gene_end": 149, "reference_start": 1, "reference_end": 149, "reference_sequence": "MQAFKEYWQKQKKDVTDKKQLLEALKLSFAKEQNKTFAFLIKNFQDGISNYYPNDQEDQSEAAKTAFGTQGIAFPQSGLKGIFMSEWLRKQLGEKAKINLDIKSLKVTDSKISPTIKWNKDIGIKRNQDKPYNFRFEIDIEYQGNYKLSWLEAIIAKFSGIPGEWKGKLNLKFIVDGDLSWEIVQKPDYPGSLFQFDDQKQQLLFKLHVWEKITVQEPEFMELIKSQNLHNLELRTESTKPPVVDLASYLHYQLLKLNQQ" }, "paralog": false, "identity": 0.98, "score": 485.0, "evalue": 8.81e-177, "description": "Internal stop codon at 625061, 625148, 625196, 625241. Nonsense mutation around 625061, 625148, 625196, 625241.", "psc": { "uniref90_id": "UniRef90_P75275", "product": "Uncharacterized protein MPN_511", "uniref50_id": "UniRef50_P75275", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75275", "UniRef:UniRef50_P75275" ] }, "pscc": { "uniref50_id": "UniRef50_P75275", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75275" ], "product": "Uncharacterized protein MPN_511" } }, "id": "APKECPOBEN_1115", "locus": "GCA002090215_01111" }, { "type": "cds", "sequence": "contig_1", "start": 625719, "stop": 625988, "strand": "-", "frame": 2, "gene": null, "product": "DUF240 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0029624419", "UniRef:UniRef90_UPI0029624419" ], "nt": "GTGTTGGGTAATAACAATGATTTAACAACTGTAGCAAAAGCAGCTGTTGGTGATAATTTATTTACCACCCAAGCTAATTTAACCAAGTCAGTTTTGGATCAAACCGTGCTAAAAGAGGCTGAATCAAGGTTTGAAGCAACTGTTTTAAAACCGTTCATTGAAGCACGGCAAAAGGCACTAGCGGAACACCAAGCACACCAAAAAGTCTTAGAGGAGCAACGCCAAAAACAGCTCGAAGAATTAAAACAAAAACAAAAGGAAGCTGAATAG", "aa": "MLGNNNDLTTVAKAAVGDNLFTTQANLTKSVLDQTVLKEAESRFEATVLKPFIEARQKALAEHQAHQKVLEEQRQKQLEELKQKQKEAE", "aa_hexdigest": "15ef2cc78a3a3556ba18ef463dc36d14", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 625770, "stop": 626063, "observations": { "mutation": [ 626012 ], "stop": [ 626012 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0029624419", "query_cov": 1.0, "subject_cov": 0.7807017543859649, "identity": 0.9890000000000001, "score": 136.0, "evalue": 7.91e-40, "gene_start": 1, "gene_end": 89, "reference_start": 26, "reference_end": 114, "reference_sequence": "MQDSLVLRGQNLTTQLFWKPTVKRLVLGNNNDLTTVAKAAVGDNLFTTQANLTKSVLDQTVLKEAESRFEATVLKPFIEARQKALAEHQAHQKVLEEQRQKQLEELKQKQKEAE" }, "paralog": false, "identity": 0.979381443298969, "score": 182.0, "evalue": 1.12e-60, "description": "Internal stop codon at 626012. Nonsense mutation around 626012.", "psc": { "uniref90_id": "UniRef90_UPI0029624419", "product": "DUF240 domain-containing protein", "uniref50_id": "UniRef50_UPI0029624419", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0029624419", "UniRef:UniRef50_UPI0029624419" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0029624419", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0029624419" ], "product": "DUF240 domain-containing protein" } }, "id": "APKECPOBEN_1116", "locus": "GCA002090215_01112" }, { "type": "cds", "sequence": "contig_1", "start": 626157, "stop": 626549, "strand": "-", "frame": 2, "gene": null, "product": "DUF240 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000A4F51D6", "UniRef:UniRef90_UPI000A4F51D6" ], "nt": "TTGCAAACTAAGTTTAAGGACAATTCGCTTAACCCACAACAGTTGGGTGTGATTGATGTTTACAACCTGTTGAGTGGATTGGAACAAAGTACGCGTGATACCGTTAAGTTAATTAATGACTTAAATGCAAAGGTATTACTAGCAAATGCCGTTTTTCCGGTGCACGAGGCATTTCAATTGCCCCAAGTACCCGAACAGTTGTTTACCATTTTGGGTAGTGATTTCTTTCCTCAATTAACCCCTAAGGGTTTAAATGTGACTGAGAATATTACGACAGTTTTGCAACAATACAAACTTCAAAGCATTGCGTTAAAGCAGTTTGATCTGAAACTAGAAAAACAACCGGACATTGTGAAGCGCTACAAGATGCGTTACAGCTTCGTAATCCAATGA", "aa": "MQTKFKDNSLNPQQLGVIDVYNLLSGLEQSTRDTVKLINDLNAKVLLANAVFPVHEAFQLPQVPEQLFTILGSDFFPQLTPKGLNVTENITTVLQQYKLQSIALKQFDLKLEKQPDIVKRYKMRYSFVIQ", "aa_hexdigest": "02e1bd1b17cff7fe0eb095975645971f", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 625978, "stop": 626810, "observations": { "deletion": [ 626072 ], "mutation": [ 626159, 626570, 626723 ], "stop": [ 626159, 626570, 626723 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000A4F51D6", "query_cov": 1.0, "subject_cov": 0.4693140794223827, "identity": 0.992, "score": 252.0, "evalue": 8.54e-83, "gene_start": 1, "gene_end": 130, "reference_start": 88, "reference_end": 217, "reference_sequence": "MKLIKKKRLLLKTLSLSLAGLTTTLAIVVWPTLTEKTNQSVSDFTLSNQRFEQNQSNRYQLQFNSGISQADLDYTTKHTNWKANFEQLQTKFKDNSLNPQQLGVIDVYNLLSGLEQSTRDTVKLINDLNAKVLLANAVFPVHEAFQLPQVPEQLFTILGSDFFPQLTPKGLNVTENITTVLQQYKLQSIALKQFDLKLEKQPDIVKRYKMRYSFVIQWDFETVYTGVNSTTNLAFSVKAMTTNFANLTGIARQLGFTWPELNYTTFLKANGQAVSVG" }, "paralog": false, "identity": 0.9855595667870036, "score": 523.0, "evalue": 9.27e-192, "description": "Internal stop codon at 626159, 626570, 626723. Frameshift due to deletion around 626072. Nonsense mutation around 626159, 626570, 626723.", "psc": { "uniref90_id": "UniRef90_UPI000A4F51D6", "product": "DUF240 domain-containing protein", "uniref50_id": "UniRef50_UPI000A4F51D6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000A4F51D6", "UniRef:UniRef50_UPI000A4F51D6" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000A4F51D6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000A4F51D6" ], "product": "DUF240 domain-containing protein" } }, "id": "APKECPOBEN_1117", "locus": "GCA002090215_01113" }, { "type": "cds", "sequence": "contig_1", "start": 626961, "stop": 627413, "strand": "-", "frame": 2, "gene": null, "product": "putative protein MPN_513", "db_xrefs": [ "RefSeq:WP_010874869.1", "SO:0001217", "UniParc:UPI000013ACFB", "UniRef:UniRef100_P75273", "UniRef:UniRef50_P75273", "UniRef:UniRef90_P75273" ], "nt": "ATGTACGACATGATTAATGCCAAGTACTTGGGCAAGTTAATCTTTTTGGATTTTGGCCAAACAATTACGAGTTTGGAAGAAACGAGTGATAACTCGACCTTTTTTATTGCTGATGAATTTGGTAATTTTGGGAGCCACTATGTGGGGGTGGTATTGGAGCAAGTCCGCTCGTTCAATACTGTAGTTATCCTGTCCTATCAATCATTTGCTAACCTGCGCAATATTGGTGGTGATAACTTTAAATCACAGATTATTAACAACACCAGTACCCTAATTGCCCACCGCTTAATTGACATGATGGAAGCAGAAGAAGTGGCTAACCTGTATGGGGTTGATATTACCTGTGCTAGTGAAATTAAAAGTTTAAAAGTGGGTCAAGCACTAGTGCGCAGTATCTCTAGTTATTATGGCGAAATCAAAGACTGGCTGGTGCAACAAATCGATAAAAGCTAA", "aa": "MYDMINAKYLGKLIFLDFGQTITSLEETSDNSTFFIADEFGNFGSHYVGVVLEQVRSFNTVVILSYQSFANLRNIGGDNFKSQIINNTSTLIAHRLIDMMEAEEVANLYGVDITCASEIKSLKVGQALVRSISSYYGEIKDWLVQQIDKS", "aa_hexdigest": "cb8379474cbdfd0cfb5bb4e6f49746f2", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013ACFB", "ncbi_nrp_id": "WP_010874869.1", "uniref100_id": "UniRef100_P75273", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013ACFB", "RefSeq:WP_010874869.1", "UniRef:UniRef100_P75273" ] }, "ips": { "uniref100_id": "UniRef100_P75273", "uniref90_id": "UniRef90_P75273" }, "psc": { "uniref90_id": "UniRef90_P75273", "product": "Uncharacterized protein MPN_513", "uniref50_id": "UniRef50_P75273" }, "pscc": { "uniref50_id": "UniRef50_P75273", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75273" ], "product": "Uncharacterized protein MPN_513" }, "genes": [], "id": "APKECPOBEN_1118", "locus": "GCA002090215_01114" }, { "type": "cds", "sequence": "contig_1", "start": 627418, "stop": 627681, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_225971167.1", "SO:0001217", "UniParc:UPI001CEF5BED", "UniRef:UniRef100_UPI001CEF5BED", "UniRef:UniRef50_A0A0H3DLB1", "UniRef:UniRef90_A0A0H3DLB1" ], "nt": "TTGTTTAAAAAAATTCACAAAGAAAAACGCCGAAAAGAAATTTTAGAAATGAAAGAAAAAGGTCGCTTTCAACCTTCCAATCAAATTTTTGTAGAAAAAATTTATGAATACTATACGGAGCATGTTCAAACCATTGACACAAAGGACTTTGAAAATTTAAGAGAAAACCTCAGGGAACTAGCGGAAATTGGTTCAATCGATTCTAATGGTGTGGGATTGATAGATATTATCAAACTGCCAAGATGCTGTTTTGTTTTCCATTAG", "aa": "MFKKIHKEKRRKEILEMKEKGRFQPSNQIFVEKIYEYYTEHVQTIDTKDFENLRENLRELAEIGSIDSNGVGLIDIIKLPRCCFVFH", "aa_hexdigest": "d282156d72d75a309d582767d643915d", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI001CEF5BED", "ncbi_nrp_id": "WP_225971167.1", "uniref100_id": "UniRef100_UPI001CEF5BED", "db_xrefs": [ "SO:0001217", "UniParc:UPI001CEF5BED", "RefSeq:WP_225971167.1", "UniRef:UniRef100_UPI001CEF5BED" ] }, "ips": { "uniref100_id": "UniRef100_UPI001CEF5BED", "uniref90_id": "UniRef90_A0A0H3DLB1" }, "psc": { "uniref90_id": "UniRef90_A0A0H3DLB1", "uniref50_id": "UniRef50_A0A0H3DLB1" }, "pscc": { "uniref50_id": "UniRef50_A0A0H3DLB1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0H3DLB1" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10476.014899999991, "isoelectric_point": 7.7838644027709964 }, "id": "APKECPOBEN_1119", "locus": "GCA002090215_01115" }, { "type": "cds", "sequence": "contig_1", "start": 627930, "stop": 628292, "strand": "-", "frame": 2, "gene": null, "product": "putative protein MPN_514", "db_xrefs": [ "RefSeq:WP_010874870.1", "SO:0001217", "UniParc:UPI000013ACFE", "UniRef:UniRef100_P75272", "UniRef:UniRef50_P75272", "UniRef:UniRef90_P75272" ], "nt": "ATGGAACAGGACATCAATAACCAGACTGGTAAGAAAATCTTTTTAAACGAGGAGTGTTTTTTGGAACTGGACAAGCTCCCTCAGCACGTCTCGGTACTAGGTGTGAGTGGTTTTGGTAAGTCCAATATCTTGCTGCACTTTTTGAAGTATGCAATTGACAACGACCATCCCTTAATTTTTGTGAATGGTAAGGGTGATAAGGAATTAATTACCCAGTTTGAACACTACCAAACGAGCGCCAAACAACTAAGTCCCGAAGACGGTTTTGATACGGTTAAGTTAGTGGCACTCAAAAATACTAGTGCCAAAATTTGGAGCTTAGATGACAGAATTGCCACGATTAAGTACAACCCGTTTAAGTAA", "aa": "MEQDINNQTGKKIFLNEECFLELDKLPQHVSVLGVSGFGKSNILLHFLKYAIDNDHPLIFVNGKGDKELITQFEHYQTSAKQLSPEDGFDTVKLVALKNTSAKIWSLDDRIATIKYNPFK", "aa_hexdigest": "2b86db1c391cf7a1ecc8ae05bfe76987", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013ACFE", "ncbi_nrp_id": "WP_010874870.1", "uniref100_id": "UniRef100_P75272", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013ACFE", "RefSeq:WP_010874870.1", "UniRef:UniRef100_P75272" ] }, "ips": { "uniref100_id": "UniRef100_P75272", "uniref90_id": "UniRef90_P75272" }, "psc": { "uniref90_id": "UniRef90_P75272", "product": "Uncharacterized protein MPN_514", "uniref50_id": "UniRef50_P75272" }, "pscc": { "uniref50_id": "UniRef50_P75272", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75272" ], "product": "Uncharacterized protein MPN_514" }, "genes": [], "id": "APKECPOBEN_1120", "locus": "GCA002090215_01116" }, { "type": "cds", "sequence": "contig_1", "start": 628454, "stop": 629977, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF05000.22", "PFAM:PF04998.22" ], "nt": "GTGAAGGAAAAGGTGTCGAGTGAGATCCAGGACTTAATTAAACGTGAAGAGTACCGTGATAACTCGATTGTTGTCATGGCCGATTCCGGGGCGCGGGGTAATATCTCCAACTTTACCCAGCTGTTTGGGATGCGGGGGTTAATGAGTAAGAGCTTTAACTATGAACGCAACAACCAGTCCAAAATTATTAAGGACACGATTGAGGTTCCTATTAAGCACTCCTTCCTGGAAGGTTTAACGATTAACGAGTACTTCAACTCATCTTATGGGGCGCGTAAGGGGATGACCGATACCGCCATGAAAACGGCTAAGTCTGGTTACATGACCCGTAAACTGGTGGATGCAACCCACGAATTGATTATTAACCACGATGACTGTGGTACACGCAAGGGAATTGTGGTCGAAGCAATTGTGGAAACCAAAACCCGTTCGTTGGTGGAATCACTGTTTGACCGCATTGTCAACCGTTACACGATTGGACCAATTTTAGATCCCGAAACCAAGGCGGAAATTGTCCCTGCTAACAGCCTAATTACCCAAGAGTTAGCCAAGCAAATTTGTGCCACTTCGATTAAACAAGTGTTAGTGCGCTCTGTGATTTACTGTGAACGCGAAAACGGCGTGTGTCAGTACTGTTTCGGGGTCGATTTGTCCACCGGCAAACTGGTGGAATTGGGTACCGCTGTGGGTGTAATTGCAGCGCAATCGATCGGTGAACCAGGAACCCAGTTAACGATGCGTACCTTCCACACCGGGGGGGTTTCGACCGAAAACAACTTGGCCCAAGGGTTTGAACGTTTAAAGCAAATCTTTGAAGTAGTCGCCCCTAAGGATTACGAGCGCTGTGTCATTTCTGAAGTTAAGGGGGTAGTTAAATCGATTACTACCACCCAAAACGCGCAGGAAGTGTTGATTGAATCTAGTGTCGATGAACGCACTTACTCGATTCCGTTTAGTGCGCAACTGCGTGTCAAGGTCGGTGATGCAGTAGAACTAGGTTCGAAGATTACCGAAGGTTCGATTGACATTCGCCAGTTATTACGGGTTGCTGGGATCCAACGGGTACGCCAGTACATGATTGTCGAAATCCAAAAGGTGTACCGTATTCAGGGGATTGAGATTGCTGATAAGTATGTCGAAATTATTATCCGCCAGTTGACGAGCTTACTCCAAGTAACCGATGCTGGCAGCAGTAACCTGTTTGTGGGTCAACTGGTACACAGTCACCACCTCAATGAACTCAACAAGAGCTTGTTGCTGTCGGGGAAGATGCCAGTGATTGCCATTAACCAGGTGTTTGGGATCGATGAGGCGGCCAGCAAGTCGAACTCCTTCTTGAGTGCCGCATCCTTCCAAGACACCAAGAAGATCTTGACCGATGCTGCGGTAAAAACCCAAGTTGACTACCTCTTAGGGTTGAAAGAAAACGTGATCATTGGGGGTAAGATCCCGGCTGGTACTGGGTTTTTAACCGATGAAGAATTGGCCTACCTCGGTGCCAAAACGGTTCAAGAGGAGTATTAA", "aa": "MKEKVSSEIQDLIKREEYRDNSIVVMADSGARGNISNFTQLFGMRGLMSKSFNYERNNQSKIIKDTIEVPIKHSFLEGLTINEYFNSSYGARKGMTDTAMKTAKSGYMTRKLVDATHELIINHDDCGTRKGIVVEAIVETKTRSLVESLFDRIVNRYTIGPILDPETKAEIVPANSLITQELAKQICATSIKQVLVRSVIYCERENGVCQYCFGVDLSTGKLVELGTAVGVIAAQSIGEPGTQLTMRTFHTGGVSTENNLAQGFERLKQIFEVVAPKDYERCVISEVKGVVKSITTTQNAQEVLIESSVDERTYSIPFSAQLRVKVGDAVELGSKITEGSIDIRQLLRVAGIQRVRQYMIVEIQKVYRIQGIEIADKYVEIIIRQLTSLLQVTDAGSSNLFVGQLVHSHHLNELNKSLLLSGKMPVIAINQVFGIDEAASKSNSFLSAASFQDTKKILTDAAVKTQVDYLLGLKENVIIGGKIPAGTGFLTDEELAYLGAKTVQEEY", "aa_hexdigest": "29f64c210d032d56c87940bbf668f5d3", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF05000.22", "name": "RNA_pol_Rpb1_4", "length": 77, "aa_cov": 0.14398422090729784, "hmm_cov": 0.987012987012987, "evalue": 5.206207549213905e-17, "score": 57.22364044189453, "start": 3, "stop": 75 }, { "id": "PF04998.22", "name": "RNA_pol_Rpb1_5", "length": 341, "aa_cov": 0.6331360946745562, "hmm_cov": 0.7536656891495601, "evalue": 6.281276824403097e-59, "score": 195.03057861328125, "start": 78, "stop": 398 } ], "seq_stats": { "molecular_weight": 56001.55770000005, "isoelectric_point": 6.254130744934082 }, "id": "APKECPOBEN_1121", "locus": "GCA002090215_01117" }, { "type": "cds", "sequence": "contig_1", "start": 629984, "stop": 632167, "strand": "-", "frame": 3, "gene": "rpoC", "product": "DNA-directed RNA polymerase subunit beta'", "db_xrefs": [ "COG:COG0086", "COG:K", "EC:2.7.7.6", "GO:0000287", "GO:0000428", "GO:0001054", "GO:0001055", "GO:0001056", "GO:0001057", "GO:0001058", "GO:0001066", "GO:0003677", "GO:0003899", "GO:0006351", "GO:0008270", "KEGG:K03046", "SO:0001217", "UniRef:UniRef50_P47582", "UniRef:UniRef90_P47582" ], "nt": "TTGTTTGATGAAGCCATTTTTGGTCCAGTGAAGGACTATGAGTGTGCTTGTGGGAAGTTTAAGAAGATTAAGTACCGTGGCGTGCGCTGTGATCGCTGTGGTGTTTGGGTAACCGAATCAATTGTGCGCCGGGAACGTATGGGCCACATTGCCCTAGTCAGTCCGGTAGCGCACATCTGGATGTCGAAGGAACTGCCTTCCCCTTCCAAGATCTCCTTAGTGTTAAACATCTCTTATAAGGAAGTGGAACAGGTCTTGTACTTTGTTAACTACATAGTATTAGATACTGGGAAGATCAAAGATCCCAAGATCATGCCGTTTAAGTTTAAGGAAGTGTTGGACTTAGCAGGCAAGGGTTCTTTAACCACGCGGCAAAAAATGCGCCGGGTGATCGGTTACATCTTCCGGAACCTGATTAAGAACAGGAGTAGCGAAGACTACCGCAAGGGGAAGATCTTTTATGAAAGCTTAAAGAACAGTTCGTTACCGTTCTCCTTAAATGATGCCTTTAACTACATTAAGAAGTACACTGGCTTCCGCGTTGGTATCGGGGCTGAAGCCATTTTAGAATTGCTCAACAAGATTGACCTTAACTATGAGTTTAGTAAGCTCAATGATGCGTTACGGAAGGCTAAGAAGGATAGTGTTGAGGATGCTAAGGTTAAAAAGATCTTGCGTCAATTGGAAACAATTAGCTGGTTTAGAAATTCGAAGCTGCACCCGAAAAACATGATCCTGCACACGGTGCCAGTGATCCCGCCAGACATCCGGCCGATTATCCAGTTAGATGGTGCTAAATTTACCACCAGTGACATTAATAACTTCTACAGAAGGGTAATTATCCGTAACGACCGTTTACGCCGCATCTTGGAAGATGGTACCGTACCTGCCATTGTGGTTAACAACGAAAAGCGCTTGTTACAAGAATCAGTTGATGCCTTGTTTGACAACTCGTCGCGCCACAAGCCAGCGTTATCCAAAGACAAACGTTCCTTGAAGTCACTCACCGACCGCTTAAAAGGGAAACAAGGGTTGTTCCGTCACAACCTTTTAGGTAAACGGGTGGACTATTCTGGTCGGAGTGTAATTGTGGTGGGTCCAGAACTAAAGATGTATGAGGTCGGGATTCCAGCATTAATGATCCTCAAGCTGTTCAAACCATTTATTATTCACGGGTTAATTAATAAGTTTGACAGCAACGGTAACGAAATTAGACCAATTGCGTCCTCGATTCGGCAAGCGGAGGACATGATTAAAAATCAGGACGACCTGATCTGGGGGATTGTGTATGATGTCATTAAGGACCGTCCGGTGTTATTAAACCGGGCACCTACTTTGCACCGTTTGGGAATACAGGCCTTTGAACCGCGGATTGTCGATGGGAAAGCGATTCGCCTACACCCGTTAGTGACCACTGCTTTTAACGCTGACTTTGACGGTGACCAGATGGCGGTGCACGTTCCGTTGAGCGAAAATGCCGTTAACGAAGCGCGTGCTATTCTGTTAGCTTCCAAGCACATCTTGGGACTAAAAGATGGCCGGCCAATTGTCACCCCAACCCAGGACATGGTGTTAGGTAACTATTACCTCACTACTGAGCGTAAGGGTCAAACGGGTGAAGGCATCATCTTTGGTACCGTGCATGAAGCCCGCGCGGCATACGAAGCGGGCAAGGTGCACCTCCACGCCATTGTCGGCATTAGCACCAAAGCCTTTCCAAACAAGCACTTTGAAGCTCAAGGTACTCTAATCACTACGGTTGGTAAGATTATCTTTAACGATGTGTTAGGGGACAACATCCCTTACATTAATGAAGGGGAATTTGATGAACATGCGTGTCCGCAAAAGTTCATAGTACCACCAAGTGGTGATGTGCGTGCTGCGATTGCCGCACACCAAGTGCTCCCTGCCTTTGGTAAGAAGGTCATCTCCAAGTTAATTGACTTACTTTACACGGTGGTTGAATTTAAGGATCTACCACGGATCTTGGAAAACATTAAGGCCTTAGGCTTTAAGTACTCGACCCACTCTTCCACCACTGTATCGGTGTTTGATATTCCGAAGTACTCCAACAAACAGCAGTACTTTGATGAAGCGGACCAACAGGTCTTGAAGTACAAGCAGTTTTACAACAAGGGTTTACTGACCGATGATGAACGGTATAAACGGGTGGTGAAACTGTGA", "aa": "MFDEAIFGPVKDYECACGKFKKIKYRGVRCDRCGVWVTESIVRRERMGHIALVSPVAHIWMSKELPSPSKISLVLNISYKEVEQVLYFVNYIVLDTGKIKDPKIMPFKFKEVLDLAGKGSLTTRQKMRRVIGYIFRNLIKNRSSEDYRKGKIFYESLKNSSLPFSLNDAFNYIKKYTGFRVGIGAEAILELLNKIDLNYEFSKLNDALRKAKKDSVEDAKVKKILRQLETISWFRNSKLHPKNMILHTVPVIPPDIRPIIQLDGAKFTTSDINNFYRRVIIRNDRLRRILEDGTVPAIVVNNEKRLLQESVDALFDNSSRHKPALSKDKRSLKSLTDRLKGKQGLFRHNLLGKRVDYSGRSVIVVGPELKMYEVGIPALMILKLFKPFIIHGLINKFDSNGNEIRPIASSIRQAEDMIKNQDDLIWGIVYDVIKDRPVLLNRAPTLHRLGIQAFEPRIVDGKAIRLHPLVTTAFNADFDGDQMAVHVPLSENAVNEARAILLASKHILGLKDGRPIVTPTQDMVLGNYYLTTERKGQTGEGIIFGTVHEARAAYEAGKVHLHAIVGISTKAFPNKHFEAQGTLITTVGKIIFNDVLGDNIPYINEGEFDEHACPQKFIVPPSGDVRAAIAAHQVLPAFGKKVISKLIDLLYTVVEFKDLPRILENIKALGFKYSTHSSTTVSVFDIPKYSNKQQYFDEADQQVLKYKQFYNKGLLTDDERYKRVVKL", "aa_hexdigest": "5e1d7fe530965636e4327be96934b350", "start_type": "TTG", "rbs_motif": "AAAA", "genes": [ "rpoC" ], "truncated": "both", "pseudogene": { "start": 629681, "stop": 632323, "observations": { "mutation": [ 629986, 632236 ], "stop": [ 629986, 632236 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47582", "query_cov": 1.0, "subject_cov": 0.5626934984520123, "identity": 0.935, "score": 1346.0, "evalue": 0.0, "gene_start": 1, "gene_end": 727, "reference_start": 56, "reference_end": 782, "reference_sequence": "MTTTRRNKRNNKLYKNIKAIKLSIASNDTILNWSEGEVTKAETINYKSLKPEPGGLFDEAIFGPVKDYECACGKFKKIKYRGVRCDRCGVWVTESIVRRERMGHIALVSPVAHIWMSKELPSPSKISLVLNISYKEVEQVLYFVNYIVLDTGKIKDDKIMPFKFKEVLDLTGKGSLSTRQKMRRVIGYIFRNLIKSKSSEDYRKGKIFYESLKNSSLPFSLNDAFNYIKKYTGFRVGIGAEAILELLNKIDLNLEFSRLNDALRKAKKDSVEDAKVKKILRQLETISWFRNSKLHPKNMILHTVPVIPPDIRPIIQLDGAKFTTSDINNFYRRVIIRNDRLRRILEDGTVPSIVVNNEKRLLQESVDALFDNSSRHKPSLSKDKRSLKSLTDRLKGKQGLFRHNLLGKRVDYSGRSVIVVGPELKMYEVGIPALMILKLFKPFIIHGLINKFDENGNEIRPIAASIRQAEDMIKNQDDLIWGIVYDVIKDRPVLLNRAPTLHRLGIQAFEPRIVDGKAIRLHPLVTTAFNADFDGDQMAVHVPLSENAVNEARAVLLASKHILGLKDGRPIVTPTQDMVLGNYYLTTERKGQLGEGIIFSTVYEARAAYESQKVHLHAIVGISTKAFPNKKFACQGTLITTVGKIIFNDVLGNNVPYINDGEFDENACPEKFIVKQGEDVRQSILKHQIIPAFSKKVISKLIDLLYLLLEFKDLPKTLDNIKALGFKYSTFSSTTVSVFDIPKYTNKQNYFDSADQQVLKYKQFYNKGLLTDDERYKRVVKLWNNVKEKVSDEIQNLIKQEQYRDNSIVVMADSGARCNISNFTQLFGMRGLMSKSFNYERNNQSKIIKDTIEVPIKHSFFEGLTINEYFNSSYGARKGMTDTAMKTAKSGYMTRKLVDATHELIINHDDCGTRKGIVVEAIVETKTKSLIESLFDRIVNRYSITPIVDPETQKTIVEANSLITTQLAKQICATSIKEVLVRSVIYCERENGICQYCFGIDLSTGKLVELGTAVGVIAAQSIGEPGTQLTMRTFHTGGVSTENNLAQGFERLKQIFEVVTPKDFEKAVISEVKGTVKSITTVQNAQEVVIKSNVDERIYTIPFSAQIRVHVGDQVSPGSKITEGSVDIKQLLRIAGIQRVRQYMIVEIQKVYRIQGIDIADKYVEIIIRQLTNLLQVTDAGNSNLFVGQLVHSHYLNELNKSLLLAGKMPVIAINQVFGIDEAASKSNSFLSAASFQDTKKILTDAAVKNQVDYLLGLKENVIIGGKIPAGTGFLTDEELTFLGSKTVAEEY" }, "paralog": false, "identity": 0.9352272727272727, "score": 1636.0, "evalue": 0.0, "description": "Internal stop codon at 629986, 632236. Nonsense mutation around 629986, 632236.", "psc": { "uniref90_id": "UniRef90_P47582", "gene": "rpoC", "product": "DNA-directed RNA polymerase subunit beta'", "ec_ids": [ "2.7.7.6" ], "uniref50_id": "UniRef50_P47582", "cog_id": "COG0086", "cog_category": "K", "kegg_orthology_id": "K03046", "go_ids": [ "GO:0000287", "GO:0000428", "GO:0001054", "GO:0001055", "GO:0001056", "GO:0001057", "GO:0001058", "GO:0001066", "GO:0003677", "GO:0003899", "GO:0006351", "GO:0008270" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47582", "EC:2.7.7.6", "UniRef:UniRef50_P47582", "COG:COG0086", "COG:K", "KEGG:K03046", "GO:0000287", "GO:0000428", "GO:0001054", "GO:0001055", "GO:0001056", "GO:0001057", "GO:0001058", "GO:0001066", "GO:0003677", "GO:0003899", "GO:0006351", "GO:0008270" ] }, "pscc": { "uniref50_id": "UniRef50_P47582", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47582" ], "product": "DNA-directed RNA polymerase subunit beta'" } }, "id": "APKECPOBEN_1122", "locus": "GCA002090215_01118" }, { "type": "cds", "sequence": "contig_1", "start": 632234, "stop": 632326, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAAAGCGTAATAAAAAGAACAACAAGCTGTACAAGAACATTAAGGCAATTAAGCTTTCGATTGCTTCCAACGACACGATCCTAAACTGA", "aa": "MTKRNKKNNKLYKNIKAIKLSIASNDTILN", "aa_hexdigest": "72f98435a436ea482b017ee4a2a46954", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3476.1007, "isoelectric_point": 10.468972587585448 }, "id": "APKECPOBEN_1123", "locus": "GCA002090215_01119" }, { "type": "cds", "sequence": "contig_1", "start": 632330, "stop": 632626, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF04560.25" ], "nt": "TTGGAAGCTTACGGCGCTGCTTACAACCTTCAGGAATTACTTACCATTAAGTCCGATGATGTGCAGGGCCGGAATAAGGCCTATGCCGCGATCGTTAAGGGCGCTGCCTTCCCCGAACCAGGTATCCCCGAATCGTTTAAACTGTTAACGAAGGAACTACAAGGGTTAGCGTTGAGTGTGTCCTTTATTTACGATGACAACACCCAACAAGATTCCAACAACGTTTCAATTCTCCAAGCTGATGGAGAACAGGACGATCTCTTTAATGACTTTGAATTTGACACGGAGGGTTATTAA", "aa": "MEAYGAAYNLQELLTIKSDDVQGRNKAYAAIVKGAAFPEPGIPESFKLLTKELQGLALSVSFIYDDNTQQDSNNVSILQADGEQDDLFNDFEFDTEGY", "aa_hexdigest": "f83241a1831aaa94eba3c9c027ad662e", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF04560.25", "name": "RNA_pol_Rpb2_7", "length": 73, "aa_cov": 0.6428571428571429, "hmm_cov": 1.0, "evalue": 3.4136345416210983e-19, "score": 64.57222747802734, "start": 1, "stop": 63 } ], "seq_stats": { "molecular_weight": 10832.72439999999, "isoelectric_point": 4.0500284194946286 }, "id": "APKECPOBEN_1124", "locus": "GCA002090215_01120" }, { "type": "cds", "sequence": "contig_1", "start": 632630, "stop": 633778, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00562.33" ], "nt": "GTGATTTCCGAGCGCTTAGTGAAGGATGATGTTCTAACCTCCTTAACGATTAACGAATACGTAGCCCAGTGTTTATCGACCAAAAACGGTGATGAACAAATTACCCGTGATATCCCCAACGTTAGTGATGCTAACAAGCGTTACTTAGATGAAAACGGCATTATCATGGTCGGTGCTGAAGTTAAGGAAGGTGATGTCCTTGTCGGTAAGGTATCCCCTAAGGGACAGGTTGAAGTATCACCTGAAGAAAAGCTGTTTAAGGCCATTTTCCCTGAAAGTGTGCAAAACGTCCGTGACTCTTCCTTAAAGCTCCCTCATGGTGGTGATGGGATTGTGTCCTGTGTCAAGCGTTTTAGCATTGCCAACGGTAATGAACTTAATGATGGTGTCATCGAAATGATTAAGGTCTATGTGGTGCAAAAACGCAAGATCCAAATCGGAGATAAGTTGGCAGGGCGCCATGGTAACAAAGGGGTTATCTCTAAGGTTGTCCCAGTAGCGGACATGCCCCACTTAGAAGATGGTACTCCAGTCGACATCCTCTTAAACCCACTGGGGGTACCGAGTCGGATGAATATTGGCCAAATCTTTGAAATGCACTTGGGTTATGCAGCACATAACCTTGCCAAGCGAATGTTAATTAGTGCTTGTTTTGATGACAAGAAGGCTCAAGCACTTTCAACCGAAATTAACCAGCCACAGTACAAACTTGACCGCTTAATTACTGGTTTAAAAGCCCAAATTACGAACCGCGGTCTTAAAGACGAGCAGGCAGCCTTGGCCCAACTCAATAACGGTGACATTGCTTTAGTGCTCAAGGAAATCGGCATGAGCTTTGATGACCTCCACTTTAAAGTAGCGACCCCGATCTTCCAAGGGGTGAACTTCCAAGACTTGCAAGACATCATGGATGAAGCAGGATTGAAACCAGCGGAAACGCACGGGAAGTTCAAGCTGATTGATGGTCGCACTGGTTTACCATTTGAAAAACCAATTTCCTTGGGAATCATGTACATTATGAAGTTAAACCACATGGTTGATGACAAGATCCATGCTCGCGCTGTCGGTCCTTACTCCAAGATTACCCAACAACCATTGGGTGGTAAATCACAAAATGGGGGCCAACGCTTTGGGGAAATGGAAGTGTGA", "aa": "MISERLVKDDVLTSLTINEYVAQCLSTKNGDEQITRDIPNVSDANKRYLDENGIIMVGAEVKEGDVLVGKVSPKGQVEVSPEEKLFKAIFPESVQNVRDSSLKLPHGGDGIVSCVKRFSIANGNELNDGVIEMIKVYVVQKRKIQIGDKLAGRHGNKGVISKVVPVADMPHLEDGTPVDILLNPLGVPSRMNIGQIFEMHLGYAAHNLAKRMLISACFDDKKAQALSTEINQPQYKLDRLITGLKAQITNRGLKDEQAALAQLNNGDIALVLKEIGMSFDDLHFKVATPIFQGVNFQDLQDIMDEAGLKPAETHGKFKLIDGRTGLPFEKPISLGIMYIMKLNHMVDDKIHARAVGPYSKITQQPLGGKSQNGGQRFGEMEV", "aa_hexdigest": "7ed30a82dd800d1cf4cab1525dc34f18", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00562.33", "name": "RNA_pol_Rpb2_6", "length": 377, "aa_cov": 0.9712041884816754, "hmm_cov": 0.7559681697612732, "evalue": 3.916690765743429e-100, "score": 330.9714050292969, "start": 1, "stop": 371 } ], "seq_stats": { "molecular_weight": 41947.88460000004, "isoelectric_point": 6.332909584045409 }, "id": "APKECPOBEN_1125", "locus": "GCA002090215_01121" }, { "type": "cds", "sequence": "contig_1", "start": 633806, "stop": 636505, "strand": "-", "frame": 3, "gene": "rpoB", "product": "DNA-directed RNA polymerase subunit beta", "db_xrefs": [ "COG:COG0085", "COG:K", "EC:2.7.7.6", "GO:0000428", "GO:0001054", "GO:0001055", "GO:0001056", "GO:0001057", "GO:0001058", "GO:0001066", "GO:0003677", "GO:0003899", "GO:0006351", "GO:0032549", "SO:0001217", "UniRef:UniRef50_P47715", "UniRef:UniRef90_P78013" ], "nt": "ATGTCACAAAAACCTAGTTTCTTTCAGAAAAAATACTCACCCACTGCTACCCGCCGTTACTATGGCAAAATTGCCACTGATTTTGTGCAACCTAATCTGGCTGATATTCAGATTAGGAGCTATCAAACTTTTCTAGATCACGATCTGGAAAACCTGATTGCTGCTTACTTTCCAATTAAGTCCCCTAATGATCGTTACACGATCAACTTTAAGGGTTTGCGTCGTACCGCTCCTGAACGTAATGAAGCCCAATCACGCTCTGAGTCCAAGACCTATGAAATTGGTATTTACGCTGACTTAGAATTAATTGATTCCGCTACCGGAACCATTAAAAAGCCGCGCAAATCCAAGAAGAACAGTGCCACCAGTAGTGTCGATGGCGTTTTCTTAACTAACTTACCATTGATTACCCGCGATGGGGTCTTTATTGTCAACGGGATTGAGAAGTTTGTGATTGCCCAAATTACCCGTTCACCGGGGATCTACATGCTCACTAAGTCCCAGTTAAAATTGTCTAGTTCGCGTAAACGGGTACAAGAAGGTTATGTATGTGAAGTATTACCAGCCAACGGTTCGGTAATGCTGATCTACATCTCCAATAAAAAGAAGATTGAAGATGCCTTTGTGCAAATCTTGCTCCGGGATGCTGTACGTGAAGGGGCAAAGATCTTCCCTATTACCACCTTGCTCAAAGCCTTTGGGATGAGTGGCAAGGAAATCCTCAAAGTTTTCAAAAACAACGAGTTTATTACCCGTTCCTTAGAAGCGGAAGTCTACAACGCTAAGGATTTCTTAAACAACGTTGACCCTGAAATTAAAAACTTATTAAGGGAATTCCGCGATGGCAAAACTGATCTCCGCCGTAAGGGGATTGCCTCTGACCAAAAGATCCGCAGCTTAGTGAGTGACTATGTCCTTTTAGAAAAGGAACACAAAGCGCTCAGTGAAGCTAAACCAAATGACCCTAAAGTAGGTCAGTTAGAAGCGGACATGGATGAGTTGATGGATAAAATCATCACCGAACGGGCTGCTAAACACATTGTTCATGAATTGTCCATTTCACTACGTGGTTTAGAAAACACCGATGAATGTCCGGAAAACAGTTACCACGCTTTACTGTGTTCACGTTTCTTCCGTCAAAGAAGGTATAACCTCTCCGCTGCTGGTCGTTATAAGGTATCACGTAAGTTAAGAATTACCGAGCGGATTTACCAAAAAACACTTGCTTGTGACCTTCATTTAAAAAACGGGGAACTCTTATTAAAGAAGGGCACCTTATTAGTTAAAGAAGAAATCGACAAGATTAAGCAAGCTGCCCAAAACAACCAAATTGACTTTGTCCAAAAGATTAAGTTAACCACTGATGGTAGTGCTGTTAACCTTTCCCCTGAATCGTTGTTGTATGAATCACTTGATGTTTATGTGAACAACGACAACTTCGATGTATCCGTGCCAGTGGTGGGGATCCACAACGATAACGATCTCAATAAGGCAATTACTTTAAGTGACTTTATTGCCTCGATTAGTTATGTGATTAACATCCCTTCGGCCATCGGTAAGTACGATGACATTGATCACTTAGGTAACAAACGCGTCAAGTTAATTAACGAGTTAATTAGTTCCCGTTTGGAAAGTGGAATCACCCGCATGGAGCGCTTCTTAAAGGAAAAGCTCACAATTGCTGATGGAGTGAACCGTGGTCAACAAATTAACGAAGAGGGTCAAGTAATTGAACAAGCGGAAAAGAAGGAATTGACCATTAAATCCTTAATTAATTCCAAACCTATTCAAATTGTCATTCGTGACTTCTTCAACACTCACCAGTTGACCCAGTTCTTAGATCACCAAAATCCACTTTCAGAATTAAGTAATAAGCGCCGGATTTCCGCCATGGGTCCAGGGGGTATCTCCCGCGAAGACCCGAACTTGGATATCCGGGATGTGCACTATTCCCAATATGGTCGGATTTGTCCAATTGAAACACCGGAAGGGATGAACATTGGTTTAATCATGTCGTTGGCTTCCTTTGCCAAGATTGACGAAAACGGATTCCTAATGGCGCCTTACCGCAAGATTAAAAATGGTGTCATTACCGATGAAGTTGAATACCTCACGGCATTAAGGGAAGACGAGCACATTATTGCCGAAATCTCTTCGTTGGTCAACATTGACGAAAACAACAAGATCTTAGATAAGGAAATTATTGGTCGTTACCGCTCGATGCAAGGGTTGTATGATCCCTCGAAGATTGACTACATCGACGTGGCCCCTCATCAGGTAGTGTCCATCGGTTCGTCCTTAATTCCCTTCTTGGAAAACGATGACTCAGCGCGGGCATTGATGGGTACCAACATGCAACGCCAAGCCTATCCTTTAATTAAGCCTTACGCTCCCGTTGTGGGTACAGGTCAAGAGTACAAGATTGCTCGCGACTCCGGCTTGACGATGTTAGCACCTTGCTCAGGTACGGTAAAGTATGTAGACAACAGTAAGATCACGATTGAAAGTGACAGTGGTGAACAGCACACTTTGGATCTAATTAAGTTTGAACGTTCCAACCAAAATACCTGCTACAACCACGTTCCCTTAGTAGAAAAGGGGCAACGGGTAACTAAGGACGAAGTGATTGCTGATGGTCCTGCAGTTAACAAGAGTGAGCTGTCTTTAGGGCAAAATGTCCTTGTCGCCTTTACGACCTGA", "aa": "MSQKPSFFQKKYSPTATRRYYGKIATDFVQPNLADIQIRSYQTFLDHDLENLIAAYFPIKSPNDRYTINFKGLRRTAPERNEAQSRSESKTYEIGIYADLELIDSATGTIKKPRKSKKNSATSSVDGVFLTNLPLITRDGVFIVNGIEKFVIAQITRSPGIYMLTKSQLKLSSSRKRVQEGYVCEVLPANGSVMLIYISNKKKIEDAFVQILLRDAVREGAKIFPITTLLKAFGMSGKEILKVFKNNEFITRSLEAEVYNAKDFLNNVDPEIKNLLREFRDGKTDLRRKGIASDQKIRSLVSDYVLLEKEHKALSEAKPNDPKVGQLEADMDELMDKIITERAAKHIVHELSISLRGLENTDECPENSYHALLCSRFFRQRRYNLSAAGRYKVSRKLRITERIYQKTLACDLHLKNGELLLKKGTLLVKEEIDKIKQAAQNNQIDFVQKIKLTTDGSAVNLSPESLLYESLDVYVNNDNFDVSVPVVGIHNDNDLNKAITLSDFIASISYVINIPSAIGKYDDIDHLGNKRVKLINELISSRLESGITRMERFLKEKLTIADGVNRGQQINEEGQVIEQAEKKELTIKSLINSKPIQIVIRDFFNTHQLTQFLDHQNPLSELSNKRRISAMGPGGISREDPNLDIRDVHYSQYGRICPIETPEGMNIGLIMSLASFAKIDENGFLMAPYRKIKNGVITDEVEYLTALREDEHIIAEISSLVNIDENNKILDKEIIGRYRSMQGLYDPSKIDYIDVAPHQVVSIGSSLIPFLENDDSARALMGTNMQRQAYPLIKPYAPVVGTGQEYKIARDSGLTMLAPCSGTVKYVDNSKITIESDSGEQHTLDLIKFERSNQNTCYNHVPLVEKGQRVTKDEVIADGPAVNKSELSLGQNVLVAFTT", "aa_hexdigest": "ba37769ed89d798322ab4f5e11afd33b", "start_type": "ATG", "rbs_motif": null, "genes": [ "rpoB" ], "truncated": "5-prime", "pseudogene": { "start": 633503, "stop": 636505, "observations": { "mutation": [ 633808 ], "stop": [ 633808 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P78013", "query_cov": 1.0, "subject_cov": 0.6458333333333334, "identity": 1.0, "score": 1738.0, "evalue": 0.0, "gene_start": 1, "gene_end": 899, "reference_start": 1, "reference_end": 899, "reference_sequence": "MSQKPSFFQKKYSPTATRRYYGKIATDFVQPNLADIQIRSYQTFLDHDLENLIAAYFPIKSPNDRYTINFKGLRRTAPERNEAQSRSESKTYEIGIYADLELIDSATGTIKKPRKSKKNSATSSVDGVFLTNLPLITRDGVFIVNGIEKFVIAQITRSPGIYMLTKSQLKLSSSRKRVQEGYVCEVLPANGSVMLIYISNKKKIEDAFVQILLRDAVREGAKIFPITTLLKAFGMSGKEILKVFKNNEFITRSLEAEVYNAKDFLNNVDPEIKNLLREFRDGKTDLRRKGIASDQKIRSLVSDYVLLEKEHKALSEAKPNDPKVGQLEADMDELMDKIITERAAKHIVHELSISLRGLENTDECPENSYHALLCSRFFRQRRYNLSAAGRYKVSRKLRITERIYQKTLACDLHLKNGELLLKKGTLLVKEEIDKIKQAAQNNQIDFVQKIKLTTDGSAVNLSPESLLYESLDVYVNNDNFDVSVPVVGIHNDNDLNKAITLSDFIASISYVINIPSAIGKYDDIDHLGNKRVKLINELISSRLESGITRMERFLKEKLTIADGVNRGQQINEEGQVIEQAEKKELTIKSLINSKPIQIVIRDFFNTHQLTQFLDHQNPLSELSNKRRISAMGPGGISREDPNLDIRDVHYSQYGRICPIETPEGMNIGLIMSLASFAKIDENGFLMAPYRKIKNGVITDEVEYLTALREDEHIIAEISSLVNIDENNKILDKEIIGRYRSMQGLYDPSKIDYIDVAPHQVVSIGSSLIPFLENDDSARALMGTNMQRQAYPLIKPYAPVVGTGQEYKIARDSGLTMLAPCSGTVKYVDNSKITIESDSGEQHTLDLIKFERSNQNTCYNHVPLVEKGQRVTKDEVIADGPAVNKSELSLGQNVLVAFTTWNGYNYEDAIVISERLVKDDVLTSLTINEYVAQCLSTKNGDEQITRDIPNVSDANKRYLDENGIIMVGAEVKEGDVLVGKVSPKGQVEVSPEEKLFKAIFPESVQNVRDSSLKLPHGGDGIVSCVKRFSIANGNELNDGVIEMIKVYVVQKRKIQIGDKLAGRHGNKGVISKVVPVADMPHLEDGTPVXDILLNPLGVPSRMNIGQIFEMHLGYAAHNLAKRMLISACFDDKKAQALSTEINQPQYKLDRLITGLKAQITNRGLKDEQAALAQLNNGDIALVLKEIGMSFDDLHFKVATPIFQGVNFQDLQDIMDEAGLKPAETHGKFKLIDGRTGLPFEKPISLGIMYMMKLNHMVDDKIHARAVGPYSKITQQPLGGKSQNGGQRFGEMEVWALEAYGAAYNLQELLTIKSDDVQGRNKAYAAIVKGAAFPEPGIPESFKLLTKELQGLALSVSFIYDDNTQQDSNNVSILQADGEQDDLFNDFEFDTEGY" }, "paralog": false, "identity": 0.999, "score": 1950.0, "evalue": 0.0, "description": "Internal stop codon at 633808. Nonsense mutation around 633808.", "psc": { "uniref90_id": "UniRef90_P78013", "gene": "rpoB", "product": "DNA-directed RNA polymerase subunit beta", "ec_ids": [ "2.7.7.6" ], "uniref50_id": "UniRef50_P47715", "cog_id": "COG0085", "cog_category": "K", "go_ids": [ "GO:0000428", "GO:0001054", "GO:0001055", "GO:0001056", "GO:0001057", "GO:0001058", "GO:0001066", "GO:0003677", "GO:0003899", "GO:0006351", "GO:0032549" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P78013", "EC:2.7.7.6", "UniRef:UniRef50_P47715", "COG:COG0085", "COG:K", "GO:0000428", "GO:0001054", "GO:0001055", "GO:0001056", "GO:0001057", "GO:0001058", "GO:0001066", "GO:0003677", "GO:0003899", "GO:0006351", "GO:0032549" ] }, "pscc": { "uniref50_id": "UniRef50_P47715", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47715" ], "product": "DNA-directed RNA polymerase subunit beta" } }, "id": "APKECPOBEN_1126", "locus": "GCA002090215_01122" }, { "type": "cds", "sequence": "contig_1", "start": 636598, "stop": 637098, "strand": "-", "frame": 1, "gene": "ssuE", "product": "putative MG342-like protein", "db_xrefs": [ "COG:C", "COG:COG0431", "GO:0005524", "GO:0005829", "GO:0010181", "GO:0016491", "RefSeq:WP_010874873.1", "SO:0001217", "UniParc:UPI000013965D", "UniRef:UniRef100_P75270", "UniRef:UniRef50_P75270", "UniRef:UniRef90_P75270" ], "nt": "ATGAGTACCAAACCGTTAATCTTATTGTTAGCTAACTCCAAAAATTCAATTAACCGGAAGTTTGCTAAAGCTTTGGAACAACAACTCAATGCTGAATTAATTGAACTAGTTGACTACCAAGTGGATTTTTATTGTGAAGATTTGGAAAAGGATCATTTTCCCGAAAAAATTAAGAGTTTAGTGCGCAAACTACACGATCACAAAACGCTAATTTTTGTCACTCCAGAACACAACGGTTTTGTCCCTGCCTTTGCTAAAAACACAATCGACTGGATGACCCGCGATACCCAATACGGTAAAAACCAGTTTTTAAAGGAGCTTGATGGAATTATTTGCTGTGTCACTCCTGCAGCTAAGAGTGGTGGTAAGACAGTGCTGGAACTACTCACTAAATTCTTTAGTTTTAGTGGTCTCAACGTTAAGGGAGCCGTGTTAGTAAATGGCTATCATGATGGCTTTGATTTTCAGCCATTTATCACTGACGTACAAAAATTGATATAA", "aa": "MSTKPLILLLANSKNSINRKFAKALEQQLNAELIELVDYQVDFYCEDLEKDHFPEKIKSLVRKLHDHKTLIFVTPEHNGFVPAFAKNTIDWMTRDTQYGKNQFLKELDGIICCVTPAAKSGGKTVLELLTKFFSFSGLNVKGAVLVNGYHDGFDFQPFITDVQKLI", "aa_hexdigest": "e2a5d7c7594bd7c1481c1e3a67847f94", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013965D", "ncbi_nrp_id": "WP_010874873.1", "uniref100_id": "UniRef100_P75270", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013965D", "RefSeq:WP_010874873.1", "UniRef:UniRef100_P75270" ] }, "ips": { "uniref100_id": "UniRef100_P75270", "uniref90_id": "UniRef90_P75270" }, "psc": { "uniref90_id": "UniRef90_P75270", "gene": "SsuE", "product": "Uncharacterized protein MG342 homolog", "uniref50_id": "UniRef50_P75270", "cog_id": "COG0431", "cog_category": "C", "go_ids": [ "GO:0005829", "GO:0005524", "GO:0010181", "GO:0016491" ] }, "pscc": { "uniref50_id": "UniRef50_P75270", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75270" ], "product": "Uncharacterized protein MG342 homolog" }, "genes": [ "ssuE" ], "id": "APKECPOBEN_1127", "locus": "GCA002090215_01123" }, { "type": "cds", "sequence": "contig_1", "start": 637525, "stop": 637743, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W6P5", "UniRef:UniRef90_A0AB38W6P5" ], "nt": "GTGAAAGTTTTGGTAGTTGTGAAACTAATAAAAAAAGATGTTTATTGTTTTCAATTCTTATTTAAGAAAAATTTAGAGGAAAACTTAGAGGATGATCATAAAGAAGAGGTATTGGATAGAATAGAAGGTTTATTCAAAAGTCTAAGAAAGGGAGGAAGCTTTAAAGAAGACGATCAGTCTAAAGATTTAATTAAGCATTTAAAAAGCATTAAAAAATGA", "aa": "MKVLVVVKLIKKDVYCFQFLFKKNLEENLEDDHKEEVLDRIEGLFKSLRKGGSFKEDDQSKDLIKHLKSIKK", "aa_hexdigest": "f9b4401fcf027851af0d30774ad05cf4", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 637333, "stop": 637743, "observations": { "mutation": [ 637368, 637437, 637527 ], "stop": [ 637368, 637437, 637527 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB38W6P5", "query_cov": 1.0, "subject_cov": 0.5255474452554745, "identity": 1.0, "score": 140.0, "evalue": 2.36e-41, "gene_start": 1, "gene_end": 72, "reference_start": 1, "reference_end": 72, "reference_sequence": "MKVLVVVKLIKKDVYCFQFLFKKNLEENLEDDHKEEVLDRIEGLFKSLRKGGSFKEDDQSKDLIKHLKSIKKWSENEDKKHLKELKKYLKSSMDTKLPSEDHWNAKEAKERLASAKKDALLELDSEWYTTFDHDKRE" }, "paralog": false, "identity": 0.9635036496350365, "score": 254.0, "evalue": 8.61e-89, "description": "Internal stop codon at 637368, 637437, 637527. Nonsense mutation around 637368, 637437, 637527.", "psc": { "uniref90_id": "UniRef90_A0AB38W6P5", "uniref50_id": "UniRef50_A0AB38W6P5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB38W6P5", "UniRef:UniRef50_A0AB38W6P5" ] }, "pscc": { "uniref50_id": "UniRef50_A0AB38W6P5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W6P5" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 8552.959799999993, "isoelectric_point": 9.015017127990724 }, "id": "APKECPOBEN_1128", "locus": "GCA002090215_01124" }, { "type": "cds", "sequence": "contig_1", "start": 637763, "stop": 637888, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0013960FD0", "UniRef:UniRef90_UPI0013960FD0" ], "nt": "ATGGAAAACTCAAAGAGAGATGAGATTAATAAGTTTTTCCGAGGGTGTGCAGCTAAAGAAGAACAAGCAAAACAAAAAAGAATTCAAGAAATTGAATTAAAAACGAAAGAAGAATTAAAAAAGTGA", "aa": "MENSKRDEINKFFRGCAAKEEQAKQKRIQEIELKTKEELKK", "aa_hexdigest": "e0e8ba66d909b53e4216456840e8ed75", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 637679, "stop": 637897, "observations": { "mutation": [ 637765 ], "stop": [ 637765 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0013960FD0", "query_cov": 1.0, "subject_cov": 0.5694444444444444, "identity": 1.0, "score": 75.9, "evalue": 2.72e-17, "gene_start": 1, "gene_end": 41, "reference_start": 4, "reference_end": 44, "reference_sequence": "MKKMENSKRDEINKFFRGCAAKEEQAKQKRIQEIELKTKEELKKWDTDKFSCESFGSCETNKKRCLLFSILI" }, "paralog": false, "identity": 0.9722222222222222, "score": 142.0, "evalue": 3.54e-46, "description": "Internal stop codon at 637765. Nonsense mutation around 637765.", "psc": { "uniref90_id": "UniRef90_UPI0013960FD0", "uniref50_id": "UniRef50_UPI0013960FD0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0013960FD0", "UniRef:UniRef50_UPI0013960FD0" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0013960FD0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0013960FD0" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 4953.655999999998, "isoelectric_point": 9.25045566558838 }, "id": "APKECPOBEN_1129", "locus": "GCA002090215_01125" }, { "type": "cds", "sequence": "contig_1", "start": 638191, "stop": 638436, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTGTATTTTGAAAACTTGCAAGTGTTTGCCCAAGAAGCTAGCACTTTACAAGTTAATGCAATTGTGATTAAACAAGATGCCTATTTTATTGTTGAAGTAATGAAGGGTTACAACTATGACAATAATTGCATCTGTTTATTAGAGGCCAATAAAGATCAAAACGTTGGTTTAACCACCGTAAGCACTAATACTCTAATAGGTGACATCAAGCAATACGAGCCTTGTTTCTTTATTGAAAAGTAA", "aa": "MLYFENLQVFAQEASTLQVNAIVIKQDAYFIVEVMKGYNYDNNCICLLEANKDQNVGLTTVSTNTLIGDIKQYEPCFFIEK", "aa_hexdigest": "b89cb279c8548b1e591f5ffe155e6a8e", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9280.518199999995, "isoelectric_point": 4.31762638092041 }, "id": "APKECPOBEN_1130", "locus": "GCA002090215_01126" }, { "type": "cds", "sequence": "contig_1", "start": 638470, "stop": 639237, "strand": "-", "frame": 1, "gene": null, "product": "putative MG343-like protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75269", "UniRef:UniRef90_P75269" ], "nt": "ATGGCACAAACAACCATTAACGAGCAAACCTTGGTGAATGAGCAAAGACTTTTAGCTTACGATTGCTTTCAAAACACCAATAAAGTGGGATTACTTTCCGCTTTAGAATACTTAGATTTTGTCAATCACCTAGTTAAGTCGAATCAGATTAAATACTTATTAGTACCATCAGCGAGTCAAACAGTTTACCAACACGTTTTCTTTGATCACTTTCGCTTTTTAAGTTTTGACAATAACTTATTAAGTGGTTTTGGTTTAGCGATTAGGTTACATAAAGAAGCGGAAACAGTGTTTATGTTGGTCGAAAAAACCAAACAAACAGAAACAGAAATTAAAAAGCTGTTAGAAGCAGTTAAACAGTTTTCCGGGCTGAAATTAGTGGTTTTACTAGTTAATTCTCCGTTTGAAAATAAGCGAACAAAGCTTTCTTATGAAATCGTTAAAATCATTAAAAAGCACGGTTATCAGTACAAACGTTATTGCCGCAAAAACTTTGAAAAGAAGTTTTTTAATACTTGGAACCACCAAAAGCGCAAGCAAAAAACAAAGAATAACCTTTTCTTTGTCGAGTTAAATTCACAGTTTGCTTTTGGCACTTCTATGGCTAACAGTAACAGTTTTGACCTGAGCTTTACCAGTTTCAAAGAACGCTTTAATACTGAAAATAACTTAGACATTGATGTGCACTTCAACGTACCACACAAGTATTTACTCAAAAACTTGGAGAGTATTAAGCACCACTTACAAATAGATAACAATACCATTTGA", "aa": "MAQTTINEQTLVNEQRLLAYDCFQNTNKVGLLSALEYLDFVNHLVKSNQIKYLLVPSASQTVYQHVFFDHFRFLSFDNNLLSGFGLAIRLHKEAETVFMLVEKTKQTETEIKKLLEAVKQFSGLKLVVLLVNSPFENKRTKLSYEIVKIIKKHGYQYKRYCRKNFEKKFFNTWNHQKRKQKTKNNLFFVELNSQFAFGTSMANSNSFDLSFTSFKERFNTENNLDIDVHFNVPHKYLLKNLESIKHHLQIDNNTI", "aa_hexdigest": "bf619e378be14f057abde21c21c8ccb1", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 638191, "stop": 639237, "observations": { "mutation": [ 638472 ], "stop": [ 638472 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75269", "query_cov": 1.0, "subject_cov": 0.7327586206896551, "identity": 1.0, "score": 492.0, "evalue": 3.64e-174, "gene_start": 1, "gene_end": 255, "reference_start": 1, "reference_end": 255, "reference_sequence": "MAQTTINEQTLVNEQRLLAYDCFQNTNKVGLLSALEYLDFVNHLVKSNQIKYLLVPSASQTVYQHVFFDHFRFLSFDNNLLSGFGLAIRLHKEAETVFMLVEKTKQTETEIKKLLEAVKQFSGLKLVVLLVNSPFENKRTKLSYEIVKIIKKHGYQYKRYCRKNFEKKFFNTWNHQKRKQKTKNNLFFVELNSQFAFGTSMANSNSFDLSFTSFKERFNTENNLDIDVHFNVPHKYLLKNLESIKHHLQIDNNTIWKQAFINFAGQLLLYFENLQVFAQEASTLQVNAIVIKQDAYFIVEVMKGYNYDNNCICLLEANKDQNVGLTTASTNTLIGDIKQYEPCFFIEK" }, "paralog": false, "identity": 0.9942528735632183, "score": 693.0, "evalue": 8.22e-256, "description": "Internal stop codon at 638472. Nonsense mutation around 638472.", "psc": { "uniref90_id": "UniRef90_P75269", "product": "Uncharacterized protein MG343 homolog", "uniref50_id": "UniRef50_P75269", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75269", "UniRef:UniRef50_P75269" ] }, "pscc": { "uniref50_id": "UniRef50_P75269", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75269" ], "product": "Uncharacterized protein MG343 homolog" } }, "id": "APKECPOBEN_1131", "locus": "GCA002090215_01127" }, { "type": "cds", "sequence": "contig_1", "start": 639240, "stop": 639548, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAGTTAGACAAGCATACTGCTATGTGCAAAAAACTCACCTTTCAGATCTTTAAGGAATTGTCGCGCTTAAACAGTGCTTATAAAACTATTACCATACCAGCCTTTTTACTGTTGGCTCAACATGATGATTTTATGCCTACAAAAGCTACTTTAAACTACTTTAACCGCTTTTTGATTAAGAAAGGTAACTTGCAATCAGGGGTTATCTTACACAGTCAACACCAAATGTTTAACAGCAAGCATGAAAGTTTCTGTAAAGCAATGCACGATATTTTGAAGCATAATAAACTAAGTAAAATTTACTAG", "aa": "MELDKHTAMCKKLTFQIFKELSRLNSAYKTITIPAFLLLAQHDDFMPTKATLNYFNRFLIKKGNLQSGVILHSQHQMFNSKHESFCKAMHDILKHNKLSKIY", "aa_hexdigest": "5dff4fa48a3661def8ac3fa04d03498e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11971.97859999999, "isoelectric_point": 9.69825305938721 }, "id": "APKECPOBEN_1132", "locus": "GCA002090215_01128" }, { "type": "cds", "sequence": "contig_1", "start": 639552, "stop": 639758, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGTGGGGCAGTGGCTAGTTATGCATTGCAGTTTTTAAAACCACAACGAGTGGAAAAACTAGTGCTCTTAGCGCCACTGTCTTATTCCAACTTGTTGAATTACTACAAGATTAAAAAGGCTTTTAAAAAGAAGGACGAAAAGCTGTCTTACTTTAAGCGGATGTTTCAACCAAAGTTTCCTGATTTACAAAACGATGGACAGTGA", "aa": "MGGAVASYALQFLKPQRVEKLVLLAPLSYSNLLNYYKIKKAFKKKDEKLSYFKRMFQPKFPDLQNDGQ", "aa_hexdigest": "5aab33eaa30b1b6da90de7cda195fd41", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7946.292999999999, "isoelectric_point": 9.889272880554199 }, "id": "APKECPOBEN_1133", "locus": "GCA002090215_01129" }, { "type": "cds", "sequence": "contig_1", "start": 639879, "stop": 640058, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAGAGACAAAGTCAATATTAATACTGTTGATATTAGCCAACTAGAAGTATTTTTTCAACCAGCTAAAAAACCCGCTAAACAAACTATTGTGTTTGCGCACGGTTTTTCAGTGCACCATTCTTATTTTAAGAGCTTTTCTGAAACGCTGGTGGATTATGACTATTACGCACCCTTGTGA", "aa": "MRDKVNINTVDISQLEVFFQPAKKPAKQTIVFAHGFSVHHSYFKSFSETLVDYDYYAPL", "aa_hexdigest": "f3be089e3822adf74f66f4f7186c04ae", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6869.7207, "isoelectric_point": 6.813312721252441 }, "id": "APKECPOBEN_1134", "locus": "GCA002090215_01130" }, { "type": "cds", "sequence": "contig_1", "start": 640061, "stop": 640465, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCTAAAAAGCCGGTTAGTGTAAACCTGTTTGCAGGACCAGCCATGTTTAAAGTTGCTAATGTTAAAGGGTTAGATCGCTTACACGAAAGCTTTAGCGCCATTAAAGACCGGGCTTATGCAGCCATTGAACAAGCACGTCAAAATGGTGTGATTACTAAAAACAACCAGGTTGTATTGACATTGGGTGTGGATTCTACTAGTGCAATTGATCAACGTCTTGTAAAACATTTAGCAGTTTGGCTAAACGTAAACCAGGTAAATATAACGAATGACCCCAACGAAATTAAGGTTGAACACACCGATCAAGTCATGTGTGAACGATGTTGGAACTTCCAAACCCAAATTTACCCTAAAAAAGGACACCAATTGTGTGCAAGGTGTTTTACTTTTCTTAAAAGGTAA", "aa": "MPKKPVSVNLFAGPAMFKVANVKGLDRLHESFSAIKDRAYAAIEQARQNGVITKNNQVVLTLGVDSTSAIDQRLVKHLAVWLNVNQVNITNDPNEIKVEHTDQVMCERCWNFQTQIYPKKGHQLCARCFTFLKR", "aa_hexdigest": "4f70531f0f68287ee56a6c328711dd4e", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15209.438499999995, "isoelectric_point": 9.474805641174317 }, "id": "APKECPOBEN_1135", "locus": "GCA002090215_01131" }, { "type": "cds", "sequence": "contig_1", "start": 640478, "stop": 640891, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08264.19" ], "nt": "GTGGACAACCGCATAGGTGACAACATTATCAAGCAAATTGTCGAGCAATACCGCCGCATTCGCAACAGCTTATTGCGCTTTATATTGGGTAACCTCAACCACTTTAACTTTGGTGAGATGAAGGATTATCGTTTTGCTCTAGAGGACAAAATCGTAATCCATAAAACTAATGCATTGGTACAAGAATTGCACCAGTGGTTAAAGCAATACAACTTTTTAAATTGCTTAAAGGCCATTAATAAGTTTGTGTTGTGGTTATCGGGTTGGTATTTTGAAATTATTAAGGACACACTTTATTGTGATGCTAAAACTAATCCTAATCGGTTGGCTAAGCAGGCAGTATTGAACTATATCTTTACCCAGTTAATTGGTTTCTTAAATATCTTTATTCCCCATACTGCCGAAGATGCTTGA", "aa": "MDNRIGDNIIKQIVEQYRRIRNSLLRFILGNLNHFNFGEMKDYRFALEDKIVIHKTNALVQELHQWLKQYNFLNCLKAINKFVLWLSGWYFEIIKDTLYCDAKTNPNRLAKQAVLNYIFTQLIGFLNIFIPHTAEDA", "aa_hexdigest": "98e8e502d76f64856c5f398eb523986b", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08264.19", "name": "Anticodon_1", "length": 89, "aa_cov": 0.6423357664233577, "hmm_cov": 0.9550561797752809, "evalue": 9.766555535281052e-18, "score": 59.92002868652344, "start": 49, "stop": 136 } ], "seq_stats": { "molecular_weight": 16338.79909999999, "isoelectric_point": 9.120100593566896 }, "id": "APKECPOBEN_1136", "locus": "GCA002090215_01132" }, { "type": "cds", "sequence": "contig_1", "start": 640895, "stop": 641377, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00133.28" ], "nt": "TTGCCGATTCCCTTAATTTATGCAGATAACCAACCCTTACTGGATGTGACTACCATTAAATACACCATTCAACAGCTCAAAAAGTACGGGATTGACAGTTGGTTTGAAAAGGATATTAATTTTTTTTTAAACCCAAAGAAAATACAGCCAGGAGTTGAATATAAAAAGGAAACTGATACCTTGGAAGTATGGTTTGACTCTGGTTCAACTTACAACGTTTTAATTAGTAATAAACTGAACTTTCCCGCTGATCTTTATTTGGAGGGTAGTGACCAATACCGCGGTTGGTTTAACTCATCCGCCAGTTGCGGTATTATCCAAACTGACCAATTACCCTTCAAGAGTTTGATTTCCCATGGTTTTACCTTGGATGAACATGGTAATAAGATGTCCAAGTCACTCGGTAATGTGGTCGACCCTTTAAAGTTGTGTGATCAGTATGGCGCAGACATTCTGCGTTTATGGGTAACTAATGTTGATTGA", "aa": "MPIPLIYADNQPLLDVTTIKYTIQQLKKYGIDSWFEKDINFFLNPKKIQPGVEYKKETDTLEVWFDSGSTYNVLISNKLNFPADLYLEGSDQYRGWFNSSASCGIIQTDQLPFKSLISHGFTLDEHGNKMSKSLGNVVDPLKLCDQYGADILRLWVTNVD", "aa_hexdigest": "cdce0c59656cff9ae2a743dd0e82c19e", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00133.28", "name": "tRNA-synt_1", "length": 167, "aa_cov": 0.98125, "hmm_cov": 0.9760479041916168, "evalue": 1.0562789509488818e-39, "score": 131.8479461669922, "start": 2, "stop": 158 } ], "seq_stats": { "molecular_weight": 18293.550699999996, "isoelectric_point": 4.967182350158691 }, "id": "APKECPOBEN_1137", "locus": "GCA002090215_01133" }, { "type": "cds", "sequence": "contig_1", "start": 641381, "stop": 641548, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00133.28" ], "nt": "GTGATTTACCGTGCCTCCAAACAGCTCTTCATTAAAACTAAATCAATTCAAAGTAAGCTAAAGCGTCAAATTAAAAGGGTTAAGTTTGTTAATAACAAGAATAAAGAGCGCCTACAAGAAATGCTGTTGCAACGTGCTGAATGGTGTATTTCCAGACAACGAGTATGA", "aa": "MIYRASKQLFIKTKSIQSKLKRQIKRVKFVNNKNKERLQEMLLQRAEWCISRQRV", "aa_hexdigest": "252389a40024f64179a19fcfbe583db8", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00133.28", "name": "tRNA-synt_1", "length": 54, "aa_cov": 0.9818181818181818, "hmm_cov": 1.0, "evalue": 4.262354894062634e-12, "score": 40.91628646850586, "start": 2, "stop": 55 } ], "seq_stats": { "molecular_weight": 6793.0944, "isoelectric_point": 11.394609642028808 }, "id": "APKECPOBEN_1138", "locus": "GCA002090215_01134" }, { "type": "cds", "sequence": "contig_1", "start": 641564, "stop": 641851, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAACCCGGTGTTGTTGGGTAATCACGTTTTATGTAATGAGGGTACAGGACTCGTGCATACTTCACCAGCTTATGGTTTGGATGACTTTTATTTGTGCAAGCAAAACAAGTTAAATGAAGCGCTAGTTTCACTTGATGAAAAAGGAGTTTTTAATGATACGTTGAACGATCCAGTTTTAACGGGTTTGTTTTATCTAAAGGCTAATGATGTGATTATTGAACGCTTAAAGCAACACCATAACTTTGTCTTTAGTGAAAGCTTTTTGCACCGCGAACCCCACGATTGA", "aa": "MNPVLLGNHVLCNEGTGLVHTSPAYGLDDFYLCKQNKLNEALVSLDEKGVFNDTLNDPVLTGLFYLKANDVIIERLKQHHNFVFSESFLHREPHD", "aa_hexdigest": "fedd404a89f9918e5212c7f1fb1d71e8", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10781.060099999992, "isoelectric_point": 5.345559501647949 }, "id": "APKECPOBEN_1139", "locus": "GCA002090215_01135" }, { "type": "cds", "sequence": "contig_1", "start": 642203, "stop": 642496, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00133.28" ], "nt": "GTGTTTATTCCTGGTTGGGATTGCCATGGTTTGCCCATAGAGCATGCGGTGAGCAAAAAAGATCCGCAACACTATGCGAGCTTATCATTGAGTGAAAAGCGCGATTTATGCAAGCAATTTGCCCTGTCACAAATTGCCATTCAAAAAGCCCAGTTTCAACGCTTAGGTCTGTTGAATGACTTTAGTAAGTATTACAAAACAATTGATGAAAGCTTTCAACAAAATGAGCTAGACCTGTTTTTACAAGCAGTGAAAAAGGATTTAATCTTCCAAGCGTTAAAACCAACTTACTGA", "aa": "MFIPGWDCHGLPIEHAVSKKDPQHYASLSLSEKRDLCKQFALSQIAIQKAQFQRLGLLNDFSKYYKTIDESFQQNELDLFLQAVKKDLIFQALKPTY", "aa_hexdigest": "6ba38bf4d506117874a4f2508a75eaf1", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00133.28", "name": "tRNA-synt_1", "length": 98, "aa_cov": 0.9690721649484536, "hmm_cov": 1.0, "evalue": 5.004820411812333e-21, "score": 70.33189392089844, "start": 2, "stop": 95 } ], "seq_stats": { "molecular_weight": 11278.85899999999, "isoelectric_point": 7.773664283752441 }, "id": "APKECPOBEN_1140", "locus": "GCA002090215_01136" }, { "type": "cds", "sequence": "contig_1", "start": 642668, "stop": 642760, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACTTAAAAAAGACACTGTTAATGCCCCAAACTGCATTTGAAATGCAGGGCAAATTAACCACTAAGGAACAGCAGTTTCAAGCTTTTTGA", "aa": "MNLKKTLLMPQTAFEMQGKLTTKEQQFQAF", "aa_hexdigest": "f6888f38c3847dcbb77891cbd9482cf2", "start_type": "ATG", "rbs_motif": "AATAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3532.1588, "isoelectric_point": 9.524639701843263 }, "id": "APKECPOBEN_1141", "locus": "GCA002090215_01137" }, { "type": "cds", "sequence": "contig_1", "start": 642803, "stop": 643201, "strand": "+", "frame": 2, "gene": "trmL", "product": "tRNA (uridine(34)/cytosine(34)/5-carboxymethylaminomethyluridine(34)-2'-O)-methyltransferase TrmL", "db_xrefs": [ "COG:COG0219", "COG:J", "EC:2.1.1.207", "GO:0002130", "GO:0003723", "GO:0005737", "GO:0006400", "GO:0008175", "GO:0141098", "GO:0141102", "KEGG:K03216", "SO:0001217", "UniRef:UniRef50_P47588", "UniRef:UniRef90_P75257" ], "nt": "ATGTATAAATCAGCGATTAATATCGTCTTATTTTGCCCAGAAATTCCTAATAACACTGGGAACATTGTTCGGAGCTGTACCGCTTTTAAAGCTAACTTGCATCTGATTAAGCCCTATGGCTTTTTTCTCAATGATAAGCGGATGGTGCGTGCTGGGTTAAATTGTTGGGATAAGGTGCAAATGTTTGAACACAAATCCTGGGAACACTTCATTGAAACAACACCACCAAATAAAGCAATTTGGTTGTTGACAAAGAGTGGTACTACTACCCCAGACCAAATTAACATGCAAACAGATAACAGGGAACAACTGTACTTTGTTTTTGGTCAAGAAACCAAGGGATTACCGCAAACACTCATGGAACAATACTCGCAGAACCAGGTACGGATTCCGATTTGA", "aa": "MYKSAINIVLFCPEIPNNTGNIVRSCTAFKANLHLIKPYGFFLNDKRMVRAGLNCWDKVQMFEHKSWEHFIETTPPNKAIWLLTKSGTTTPDQINMQTDNREQLYFVFGQETKGLPQTLMEQYSQNQVRIPI", "aa_hexdigest": "f6f3fa676df99b31653d2ad76cc1e762", "start_type": "ATG", "rbs_motif": null, "genes": [ "trmL" ], "truncated": "3-prime", "pseudogene": { "start": 642803, "stop": 643303, "observations": { "mutation": [ 643199 ], "stop": [ 643199 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75257", "query_cov": 1.0, "subject_cov": 0.7951807228915663, "identity": 1.0, "score": 283.0, "evalue": 1.49e-96, "gene_start": 1, "gene_end": 132, "reference_start": 1, "reference_end": 132, "reference_sequence": "MYKSAINIVLFCPEIPNNTGNIVRSCTAFKANLHLIKPYGFFLNDKRMVRAGLNCWDKVQMFEHKSWEHFIETTPPNKAIWLLTKSGTTTPDQINMQTDNREQLYFVFGQETKGLPQTLMEQYSQNQVRIPIWNPVRSINLSNTVACVLYEYAKQNHYFNLDKQCA" }, "paralog": false, "identity": 0.9939759036144579, "score": 347.0, "evalue": 3.38e-124, "description": "Internal stop codon at 643199. Nonsense mutation around 643199.", "psc": { "uniref90_id": "UniRef90_P75257", "gene": "trmL", "product": "tRNA (uridine(34)/cytosine(34)/5-carboxymethylaminomethyluridine(34)-2'-O)-methyltransferase TrmL", "ec_ids": [ "2.1.1.207" ], "uniref50_id": "UniRef50_P47588", "cog_id": "COG0219", "cog_category": "J", "kegg_orthology_id": "K03216", "go_ids": [ "GO:0002130", "GO:0003723", "GO:0005737", "GO:0006400", "GO:0008175", "GO:0141098", "GO:0141102" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75257", "EC:2.1.1.207", "UniRef:UniRef50_P47588", "COG:COG0219", "COG:J", "KEGG:K03216", "GO:0002130", "GO:0003723", "GO:0005737", "GO:0006400", "GO:0008175", "GO:0141098", "GO:0141102" ] }, "pscc": { "uniref50_id": "UniRef50_P47588", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47588" ], "product": "Putative tRNA (cytidine(34)-2'-O)-methyltransferase" } }, "id": "APKECPOBEN_1142", "locus": "GCA002090215_01138" }, { "type": "cds", "sequence": "contig_1", "start": 643294, "stop": 643635, "strand": "+", "frame": 1, "gene": "trmB", "product": "tRNA (guanosine(46)-N7)-methyltransferase TrmB", "db_xrefs": [ "COG:COG0220", "COG:J", "EC:2.1.1.33", "GO:0006400", "GO:0008176", "GO:0043527", "SO:0001217", "UniRef:UniRef50_P47589", "UniRef:UniRef90_P75256" ], "nt": "GTGCGCTTAAGAAAAGTTAAAGATGCTCTTGTAAGGGTACAAGACAGCCCTTACTTTAGTAACCCAGAACAACTGTCAACTATTGAAAGTGAATCGCTCTTCGTGGAAATAGGTTGTGGTAAAAGTTGGTTTTTAAGCCAGCAAGCACAGCAAAACCCAAAGTGTTTTTTTGTGGGAATCGAGCGTGAACCCACGATTGTTTTAAAGGCAATTAATAAGGTAAACCGCTTAGAGGTTAAACCACGTAATTTATTAATAACGTGTTTGGATGCCAACCAGCTAACAGAGTACTTAAAACCACAATCGATTAGCAAAATCTTCATTAACTTCCCCGATCCTTGA", "aa": "MRLRKVKDALVRVQDSPYFSNPEQLSTIESESLFVEIGCGKSWFLSQQAQQNPKCFFVGIEREPTIVLKAINKVNRLEVKPRNLLITCLDANQLTEYLKPQSISKIFINFPDP", "aa_hexdigest": "51c22b221694814d10f5d606558cc1e1", "start_type": "GTG", "rbs_motif": null, "genes": [ "trmB" ], "truncated": "3-prime", "pseudogene": { "start": 643294, "stop": 643926, "observations": { "mutation": [ 643633 ], "stop": [ 643633 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75256", "query_cov": 1.0, "subject_cov": 0.5380952380952381, "identity": 1.0, "score": 229.0, "evalue": 4.51e-75, "gene_start": 1, "gene_end": 113, "reference_start": 1, "reference_end": 113, "reference_sequence": "MRLRKVKDALVRVQDSPYFSNPEQLSTIESESLFVEIGCGKSWFLSQQAQQNPKCFFVGIEREPTIVLKAINKVNRLEVKPRNLLITCLDANQLTEYLKPQSISKIFINFPDPWPKKRHTLRRLTAPHFLKQFHQLLKKQGLIEFKTDNDQLFEFTLEVLQKTVAHFKIIEQTTDLHNSPLTSTNIMTEYEQRFVSLGVKIKKLTLEKLN" }, "paralog": false, "identity": 0.9904761904761905, "score": 414.0, "evalue": 3.73e-150, "description": "Internal stop codon at 643633. Nonsense mutation around 643633.", "psc": { "uniref90_id": "UniRef90_P75256", "gene": "trmB", "product": "tRNA (guanosine(46)-N7)-methyltransferase TrmB", "ec_ids": [ "2.1.1.33" ], "uniref50_id": "UniRef50_P47589", "cog_id": "COG0220", "cog_category": "J", "go_ids": [ "GO:0006400", "GO:0008176", "GO:0043527" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75256", "EC:2.1.1.33", "UniRef:UniRef50_P47589", "COG:COG0220", "COG:J", "GO:0006400", "GO:0008176", "GO:0043527" ] }, "pscc": { "uniref50_id": "UniRef50_P47589", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47589" ], "product": "tRNA (guanine-N(7)-)-methyltransferase" } }, "id": "APKECPOBEN_1143", "locus": "GCA002090215_01139" }, { "type": "cds", "sequence": "contig_1", "start": 643654, "stop": 643926, "strand": "+", "frame": 1, "gene": "trmB", "product": "tRNA (guanosine(46)-N7)-methyltransferase TrmB", "db_xrefs": [ "COG:COG0220", "COG:J", "EC:2.1.1.33", "GO:0006400", "GO:0008176", "GO:0043527", "SO:0001217", "UniRef:UniRef50_P47589", "UniRef:UniRef90_P75256" ], "nt": "TTGAGGCGACTAACTGCTCCACATTTCCTCAAGCAGTTTCACCAACTTTTAAAAAAGCAAGGTTTAATAGAATTCAAAACGGACAACGATCAGTTGTTTGAATTTACCTTAGAAGTCTTGCAGAAAACAGTAGCTCACTTTAAGATAATTGAGCAAACGACAGATCTGCACAATTCACCTTTGACTAGTACCAATATTATGACCGAATACGAACAGCGCTTTGTTAGTTTAGGGGTTAAGATTAAAAAGCTAACGCTTGAAAAACTAAATTAG", "aa": "MRRLTAPHFLKQFHQLLKKQGLIEFKTDNDQLFEFTLEVLQKTVAHFKIIEQTTDLHNSPLTSTNIMTEYEQRFVSLGVKIKKLTLEKLN", "aa_hexdigest": "1c22775d4ebee013521eb50beb379408", "start_type": "TTG", "rbs_motif": null, "genes": [ "trmB" ], "truncated": "5-prime", "pseudogene": { "start": 643354, "stop": 643926, "observations": { "mutation": [ 643633 ], "stop": [ 643633 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75256", "query_cov": 1.0, "subject_cov": 0.42857142857142855, "identity": 0.9890000000000001, "score": 177.0, "evalue": 8.31e-55, "gene_start": 1, "gene_end": 90, "reference_start": 121, "reference_end": 210, "reference_sequence": "MRLRKVKDALVRVQDSPYFSNPEQLSTIESESLFVEIGCGKSWFLSQQAQQNPKCFFVGIEREPTIVLKAINKVNRLEVKPRNLLITCLDANQLTEYLKPQSISKIFINFPDPWPKKRHTLRRLTAPHFLKQFHQLLKKQGLIEFKTDNDQLFEFTLEVLQKTVAHFKIIEQTTDLHNSPLTSTNIMTEYEQRFVSLGVKIKKLTLEKLN" }, "paralog": false, "identity": 0.9947368421052631, "score": 377.0, "evalue": 4.49e-136, "description": "Internal stop codon at 643633. Nonsense mutation around 643633.", "psc": { "uniref90_id": "UniRef90_P75256", "gene": "trmB", "product": "tRNA (guanosine(46)-N7)-methyltransferase TrmB", "ec_ids": [ "2.1.1.33" ], "uniref50_id": "UniRef50_P47589", "cog_id": "COG0220", "cog_category": "J", "go_ids": [ "GO:0006400", "GO:0008176", "GO:0043527" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75256", "EC:2.1.1.33", "UniRef:UniRef50_P47589", "COG:COG0220", "COG:J", "GO:0006400", "GO:0008176", "GO:0043527" ] }, "pscc": { "uniref50_id": "UniRef50_P47589", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47589" ], "product": "tRNA (guanine-N(7)-)-methyltransferase" } }, "id": "APKECPOBEN_1144", "locus": "GCA002090215_01140" }, { "type": "tRNA", "sequence": "contig_1", "start": 643956, "stop": 644031, "strand": "+", "gene": "trnH", "product": "tRNA-His(gtg)", "amino_acid": "His", "anti_codon": "gtg", "score": 71.9, "nt": "GCGATTGTGGCGAAGTGGTTAACGCACCTGATTGTGGATCAGGCATTCGTGGGTTCAATTCCCATCAGTCGCCCCA", "db_xrefs": [ "SO:0000262" ], "anti_codon_pos": [ 643962, 643964 ], "id": "APKECPOBEN_16", "locus": "GCA002090215_01141" }, { "type": "cds", "sequence": "contig_1", "start": 644081, "stop": 644446, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGACTTTTATTCAAACAGTTTACAGCGGTAGTGTCAATAATCTTATTAACCAGTCCAATGTTCATGCCCAAACGGTTGGCACTGATGTAACTAAAGCAATTGTGATTGCTTTTCGCAAAAACAATTTAGATCAAATTAGTGCGCATTTCTATAATCCTGATAACAGTAACGGCAGTAATGCACCAGGTTCAAACCAACCAAATCAGGACAGTGGAAATAACGGTTCAACAACTCCAGCAGCTCCTGCTGCAGCCGCCGCTAAAAAATCCTATTCAGCTGGATCATTTGGCGTTAAACGCCATGCCGTGCAGGTAAGCATTAACTTTCTCAATTTCTTGGATAGTGTTTACACACCCTTAAACTAG", "aa": "MTFIQTVYSGSVNNLINQSNVHAQTVGTDVTKAIVIAFRKNNLDQISAHFYNPDNSNGSNAPGSNQPNQDSGNNGSTTPAAPAAAAAKKSYSAGSFGVKRHAVQVSINFLNFLDSVYTPLN", "aa_hexdigest": "0fcea345e36f9b8529eec0172f7e4236", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12759.832499999982, "isoelectric_point": 9.1006311416626 }, "id": "APKECPOBEN_1145", "locus": "GCA002090215_01142" }, { "type": "cds", "sequence": "contig_1", "start": 644678, "stop": 644998, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGAAGTGGTTTAGTTCAATTTCTAAAAAGAAAGTATCTTTTTCAACCTTACTCTTGTTAGGTTCAGGAATTGTTTTGAGTTCCTGTAGCAATATTGATAAACCAAATGTTTTTCGCACTCTTTCACAGCAAAGCGTAGAAAACAAAGTTGATTACAGTAAGCTACCTAAAGAAAATAAAACGGTTAGAAACTTAGTTTTTGGAACAGCGGAATACAATGATGGTAACTACGTTCTTGTAGTAACCACTGAAACTGACAGTTCTCAAATTAACTTTCTAAATGGTTCAAACAATCAAGCTGTTAGTACCGAAAATTGA", "aa": "MKKWFSSISKKKVSFSTLLLLGSGIVLSSCSNIDKPNVFRTLSQQSVENKVDYSKLPKENKTVRNLVFGTAEYNDGNYVLVVTTETDSSQINFLNGSNNQAVSTEN", "aa_hexdigest": "f436f68e8794e7874d1fb98a8b981eb7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11744.072799999993, "isoelectric_point": 9.09850368499756 }, "id": "APKECPOBEN_1146", "locus": "GCA002090215_01143" }, { "type": "tRNA", "sequence": "contig_1", "start": 645013, "stop": 645098, "strand": "-", "gene": "trnL", "product": "tRNA-Leu(gag)", "amino_acid": "Leu", "anti_codon": "gag", "score": 65.6, "nt": "GTCGGAGTGGTGGAATGGTAGACACGCAAGCTTGAGGGGCTTGTGGTCGCAAGACTGTGCCAGTTCAAGTCTGGTCTCCGACACCA", "db_xrefs": [ "SO:0000264" ], "anti_codon_pos": [ 645092, 645094 ], "id": "APKECPOBEN_19", "locus": "GCA002090215_01144" }, { "type": "cds", "sequence": "contig_1", "start": 645588, "stop": 645947, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01519.22" ], "nt": "GTGAAAGTTAAATACGTTACCCAAGAACAATTTCAGGAATATAAAGATTCTAACAATCAGCGACTCATAAAGATTGAAACCACTTTGGCGGCCCAAGGTGAGCAAATAAGAATCCAGGTTGAGCAAATTAAAGAACTTCAAATCGAGCAAAAAGCTCAGGGCGAAACCTTAAAGCTAATCTTGCAAACGCTGCAAAAGATGAGCGATCGCTTGGACAAGATGGAAGTAAAAATGGACAAGATGGAAGAAAAAATGGACAAGATGGAAGGAAAAATAGACAAAATAGAAAATAGAATGGACAAGATGGAAGTAAAAATGGACAAAATGGAAAAAAGAATAGACAAACTTGAGTCCAAATAA", "aa": "MKVKYVTQEQFQEYKDSNNQRLIKIETTLAAQGEQIRIQVEQIKELQIEQKAQGETLKLILQTLQKMSDRLDKMEVKMDKMEEKMDKMEGKIDKIENRMDKMEVKMDKMEKRIDKLESK", "aa_hexdigest": "129b6ba801580606e6e1bba469a6b057", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01519.22", "name": "DUF16", "length": 88, "aa_cov": 0.6218487394957983, "hmm_cov": 1.0, "evalue": 8.623647535187092e-26, "score": 85.96153259277344, "start": 2, "stop": 75 } ], "seq_stats": { "molecular_weight": 14313.624699999993, "isoelectric_point": 7.801722145080567 }, "id": "APKECPOBEN_1147", "locus": "GCA002090215_01145" }, { "type": "cds", "sequence": "contig_1", "start": 645984, "stop": 646094, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGAAAAAATTTCCGAAAAGGAATACAAAGCCTTAATCCGTAAAATTGGAAAAGAACACTTTGATGGTGAAAAAGAAGAGTACGGTGATGGCACAGTTGGAGTTTGA", "aa": "MKEKISEKEYKALIRKIGKEHFDGEKEEYGDGTVGV", "aa_hexdigest": "7ebb4537de1704cf08275a6cca7d1498", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4142.643400000003, "isoelectric_point": 5.72024211883545 }, "id": "APKECPOBEN_1148", "locus": "GCA002090215_01146" }, { "type": "cds", "sequence": "contig_1", "start": 646687, "stop": 646803, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAGCCGAACTATTACCGTGTCATCAAGAAAGCAACCTCATTTTCAGGGTTAGATATGATCTCTCATGCCTATGGTCAGATTGCTGGCGTTGAAGTTGTGGGTTTTTATTTATGA", "aa": "MQPNYYRVIKKATSFSGLDMISHAYGQIAGVEVVGFYL", "aa_hexdigest": "b5707dbd8c72970a7499dee8c5e630f8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4254.8816, "isoelectric_point": 8.119486808776855 }, "id": "APKECPOBEN_1149", "locus": "GCA002090215_01147" }, { "type": "cds", "sequence": "contig_1", "start": 647071, "stop": 647673, "strand": "+", "frame": 1, "gene": "dnaB2", "product": "putative MG349-like protein", "db_xrefs": [ "COG:COG3611", "COG:L", "KEGG:K03346", "SO:0001217", "UniRef:UniRef50_P75253", "UniRef:UniRef90_P75253" ], "nt": "ATGGGAAAACTGGAATATGACCGCAACTGCCTTTCCTTTGAACAAATCGATAACATTCAGTTTGACAACGCACTGGAAATTACCGCTAACTTTGAAGCTAACTTTACCAGTAAGCAAGATTGTCTTAGCTTTAGTTTTGACTTTGAAGCTTTTCATAAACAATTAGTTAAACAAAATCTCTTTGTTAGTTTTAACCAGAAAACTAAGGCTGTTATTAATGGTTACTTTGAAAAATACCAAATAACACTAGAAGGCATATTGGAATGTGTTATCCCTAGTGTAGTTAATGATGAAGTTGACTTGGAACTGCTACAAAAATTACTAGAGCAGCTAGTTAAAAACAACACAGCTCCAATTGTTGATACTGTAATAAATGACCGCAACTTCTTTTATGATAATCAGAATTTAGCCACAGAAACAAAAGATGCAATTTCCCGTTGTCATTTGGAATACAACGCGGAAAAATACCTATTTCTCTTGTACGGTAAAGTAGAAAACGAGCAGTTAGATCTAATTCGCCGCTTAAGACAAGAGTTTGGAATAGCTGATAAGGTAATTAATCTAATAGTAGACTTCTCCTTTTGGAAAAACAACAGCATGTGA", "aa": "MGKLEYDRNCLSFEQIDNIQFDNALEITANFEANFTSKQDCLSFSFDFEAFHKQLVKQNLFVSFNQKTKAVINGYFEKYQITLEGILECVIPSVVNDEVDLELLQKLLEQLVKNNTAPIVDTVINDRNFFYDNQNLATETKDAISRCHLEYNAEKYLFLLYGKVENEQLDLIRRLRQEFGIADKVINLIVDFSFWKNNSM", "aa_hexdigest": "7ea46e9cacdd831ddf76e2dc8f905020", "start_type": "ATG", "rbs_motif": "AAAA", "genes": [ "dnaB2" ], "truncated": "both", "pseudogene": { "start": 646771, "stop": 647928, "observations": { "mutation": [ 646801, 646915, 646999, 647020, 647671 ], "stop": [ 646801, 646915, 646999, 647020, 647671 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75253", "query_cov": 1.0, "subject_cov": 0.48426150121065376, "identity": 1.0, "score": 398.0, "evalue": 4.93e-137, "gene_start": 1, "gene_end": 200, "reference_start": 129, "reference_end": 328, "reference_sequence": "MQPNYYRVIKKATSFSGLDMISHAYGQIAGVEVVGFYLWLITEANAQAFNSEIRTPISRLQNAFNTTGTKNVNVPDWIYKTIGKLESLGLVRTFFSQEKSEMTFWIVEPLGWKEFNQKKHFKEKLIESMGKLEYDRNCLSFEQIDNIQFDNALEITANFEANFTSKQDCLSFSFDFEAFHKQLVKQNLFVSFNQKTKAVINGYFEKYQITLEGILECVIPSVVNDEVDLELLQKLLEQLVKNNTAPIVDTVINDRNFFYDNQNLATETKDAISRCHLEYNAEKYLFLLYGKVENEQLDLIRRLRQEFGIADKVINLIVDFSFWKNNSMWREQYILKIAESVERYRSHNNYQATLDNFIRASSLAKKQRTKTKIEKSEPETSAAPVNETDDVNYFLNRIKAINKKNRENGKHKR" }, "paralog": false, "identity": 0.987012987012987, "score": 744.0, "evalue": 4.12e-276, "description": "Internal stop codon at 646801, 646915, 646999, 647020, 647671. Nonsense mutation around 646801, 646915, 646999, 647020, 647671.", "psc": { "uniref90_id": "UniRef90_P75253", "gene": "DnaB2", "product": "Uncharacterized protein MG349 homolog", "uniref50_id": "UniRef50_P75253", "cog_id": "COG3611", "cog_category": "L", "kegg_orthology_id": "K03346", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75253", "UniRef:UniRef50_P75253", "COG:COG3611", "COG:L", "KEGG:K03346" ] }, "pscc": { "uniref50_id": "UniRef50_P75253", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75253" ], "product": "Uncharacterized protein MG349 homolog" } }, "id": "APKECPOBEN_1150", "locus": "GCA002090215_01148" }, { "type": "cds", "sequence": "contig_1", "start": 647909, "stop": 648211, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAAACACAAACGCTAAAACTCAACCAGCAATAAATGAGTTAGATAAGACTACAGTTCAAGACACAATTAAGAATTACGAACAACAACTTCAAAAAGAGCTTAATGAAGAACAACTAAAAGCTTTTAAAAAGCGCTTTAAGTATTTGAAGCTAACTAAAAGAGAAGTGATTGAAAAGGAAAGCAGGGAACTTTTATTGCAAGCATTTAGAGACTTTGAAAATTGTGAAAAGATAAAAGGCAATCATTGTCCTAATAAAGGCAATGCTCACCTCAACATCTTTCGCGAGCCACCTTTTTGA", "aa": "MENTNAKTQPAINELDKTTVQDTIKNYEQQLQKELNEEQLKAFKKRFKYLKLTKREVIEKESRELLLQAFRDFENCEKIKGNHCPNKGNAHLNIFREPPF", "aa_hexdigest": "e74725482ce126c15413da07ea46fdf6", "start_type": "ATG", "rbs_motif": "AACAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11923.459799999984, "isoelectric_point": 8.885951423645022 }, "id": "APKECPOBEN_1151", "locus": "GCA002090215_01149" }, { "type": "tRNA", "sequence": "contig_1", "start": 648955, "stop": 649036, "strand": "+", "gene": "trnL", "product": "tRNA-Leu(caa)", "amino_acid": "Leu", "anti_codon": "caa", "score": 59.5, "nt": "CCTGGAGTGGCGGAATGGTAGACGCGGTGGACTCAAAACCCACTAGGAAACTGTAGGAGTTCAAGTCTCCTCTCCAGGACCA", "db_xrefs": [ "SO:0000264" ], "anti_codon_pos": [ 648988, 648990 ], "id": "APKECPOBEN_17", "locus": "GCA002090215_01150" }, { "type": "cds", "sequence": "contig_1", "start": 649174, "stop": 649518, "strand": "-", "frame": 1, "gene": null, "product": "MPN527 family putative ECF transporter permease subunit", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_Q9ZB72", "UniRef:UniRef90_P75251" ], "nt": "GTGAGTTTTACACGGTTGTACTTTCACATTTTTGCTCAAATTAAACTTCGCTGGTTATGGGTATTTAGTTTGGCTACTCTATCTAACACTTTGCTGTTAACAACGCTCAACGGTTTGTTAATTACCCCTTTATACTGGTATTGGTTTGGCTATGTGCCGACTGCTAACTTTGTCGAAGTGGCCAAGATATATAACAAAACACCATATTTTCACTTCTTTTTATTTGGCGTACCTAATTATTGGGGTGGCATCTTTGCTTTATACTCACTCTTTAATGTGATTAAATTCACACTAGTGAGTTTAATTGGTGTTCCAGTGATGCGTGCTTTCCAAAAGTTTTACTGA", "aa": "MSFTRLYFHIFAQIKLRWLWVFSLATLSNTLLLTTLNGLLITPLYWYWFGYVPTANFVEVAKIYNKTPYFHFFLFGVPNYWGGIFALYSLFNVIKFTLVSLIGVPVMRAFQKFY", "aa_hexdigest": "8d5d8f4facd413fa0442d661665c76b3", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 649150, "stop": 649818, "observations": { "mutation": [ 649176, 649620 ], "stop": [ 649176, 649620 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75251", "query_cov": 0.9912280701754386, "subject_cov": 0.5022222222222222, "identity": 1.0, "score": 232.0, "evalue": 9.37e-76, "gene_start": 2, "gene_end": 114, "reference_start": 105, "reference_end": 217, "reference_sequence": "MNGARIAFWPKKEQHQLFNLSFSAMMLALALIASFVSHFISIPFLSALKLTIDISSVFLIACAFFVSYSWALVITVALSLCSFIWDGNNWIGILTLTIANFAIVSFTRLYFHIFAQIKLRWLWVFSLATLSNTLLLTTLNGLLITPLYWYWFGYVPTANFVEVAKIYNKTPYFHFFLFGVPNYWGGIFALYSLFNVIKFTLVSLIGVPVMRAFQKFYWKKAQIVY" }, "paralog": false, "identity": 0.990990990990991, "score": 442.0, "evalue": 5.09e-161, "description": "Internal stop codon at 649176, 649620. Nonsense mutation around 649176, 649620.", "psc": { "uniref90_id": "UniRef90_P75251", "product": "MPN527 family putative ECF transporter permease subunit", "uniref50_id": "UniRef50_Q9ZB72", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75251", "UniRef:UniRef50_Q9ZB72", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_Q9ZB72", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9ZB72" ], "product": "Uncharacterized protein MG350.1" } }, "id": "APKECPOBEN_1152", "locus": "GCA002090215_01151" }, { "type": "cds", "sequence": "contig_1", "start": 649618, "stop": 649827, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGGTGCAAGAATAGCTTTTTGGCCAAAAAAAGAACAACACCAACTGTTTAACCTTAGTTTTAGTGCCATGATGCTAGCGCTTGCACTAATTGCTTCGTTTGTTAGTCACTTTATCAGTATTCCGTTTTTGTCAGCACTTAAATTAACGATTGACATTTCATCAGTCTTTTTAATTGCTTGTGCTTTTTTTGTTTCTTATTCTTGA", "aa": "MNGARIAFWPKKEQHQLFNLSFSAMMLALALIASFVSHFISIPFLSALKLTIDISSVFLIACAFFVSYS", "aa_hexdigest": "550e5d77e2c6ceb6ad32534136d2f50c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7703.113999999996, "isoelectric_point": 9.1023717880249 }, "id": "APKECPOBEN_1153", "locus": "GCA002090215_01152" }, { "type": "cds", "sequence": "contig_1", "start": 649882, "stop": 650436, "strand": "+", "frame": 1, "gene": "ppa", "product": "Inorganic pyrophosphatase", "db_xrefs": [ "COG:C", "COG:COG0221", "EC:3.6.1.1", "GO:0000287", "GO:0004427", "GO:0005737", "GO:0006796", "RefSeq:WP_010874884.1", "SO:0001217", "UniParc:UPI000012D841", "UniRef:UniRef100_P75250", "UniRef:UniRef50_P47593", "UniRef:UniRef90_P75250" ], "nt": "ATGGACAAATTTTTAATCGATGTTACTGTAGAAATTCCTAAGAGCAGTAAGATCAAGTATGAATACGACCGCAAGACTAGTCAAATCCGTGTCGACAGGATTCTCTTTGGTAGTGAATCTTATCCACAAAACTATGGTTTTATCGCTAACACCTTAGACTGGGATGGTGATGAATTGGACTGTTTTATCTTCGCTGACCAAGCCTTTCTACCAGGTGTAGTAGTACCTACTAGAATTGTAGGAGCTTTAGAAATGGTAGATGACGGTGAATTGGATACCAAACTTTTAGGGGTCATTGATTGTGATCCGCGTTACAAAGAGATTAACTCCGTCAATGATTTACCAAAACACCGCGTTGATGAAATTATTGGTTTTCTCAAAACTTACAAATTACTACAAAAGAAAGAAGTAATTATTAAGGGAGTGCAAAGTTTGGAGTGGGCTAAGAAAGAATACCAAGTGTGTGTTGATTTAATGAAGCAATATGGTAAGTTGCCTAAGGATGAGTTTATCGCTAAAATGCAAAAACTCCATCCTGAACACTACCAAAAATAG", "aa": "MDKFLIDVTVEIPKSSKIKYEYDRKTSQIRVDRILFGSESYPQNYGFIANTLDWDGDELDCFIFADQAFLPGVVVPTRIVGALEMVDDGELDTKLLGVIDCDPRYKEINSVNDLPKHRVDEIIGFLKTYKLLQKKEVIIKGVQSLEWAKKEYQVCVDLMKQYGKLPKDEFIAKMQKLHPEHYQK", "aa_hexdigest": "0a734c793bda5d5833afdf32071f2525", "start_type": "ATG", "rbs_motif": "AATAA", "ups": { "uniparc_id": "UPI000012D841", "ncbi_nrp_id": "WP_010874884.1", "uniref100_id": "UniRef100_P75250", "db_xrefs": [ "SO:0001217", "UniParc:UPI000012D841", "RefSeq:WP_010874884.1", "UniRef:UniRef100_P75250" ] }, "ips": { "uniref100_id": "UniRef100_P75250", "uniref90_id": "UniRef90_P75250" }, "psc": { "uniref90_id": "UniRef90_P75250", "gene": "ppa", "product": "Inorganic pyrophosphatase", "ec_ids": [ "3.6.1.1" ], "uniref50_id": "UniRef50_P47593", "cog_id": "COG0221", "cog_category": "C", "go_ids": [ "GO:0005737", "GO:0004427", "GO:0000287", "GO:0006796" ] }, "pscc": { "uniref50_id": "UniRef50_P47593", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47593" ], "product": "Inorganic pyrophosphatase" }, "genes": [ "ppa" ], "id": "APKECPOBEN_1154", "locus": "GCA002090215_01153" }, { "type": "cds", "sequence": "contig_1", "start": 650763, "stop": 650963, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03838.20" ], "nt": "TTGTTAATCTATTTTCAGGTACAAGACCTCTTATATGCGTTGCATACCAAGGATCTCTTGCAGATGATCGAAGCGCAAAAGGGTAAAAGTAAAACAATTAAGCGTGACCTTATTGATCAAAAAGCACAAAAGATAAATCTAATCTACCCAGGGGTTATAGATCTAATTGATGTAATAGAAAACTTTATAAAAGACTCTTAG", "aa": "MLIYFQVQDLLYALHTKDLLQMIEAQKGKSKTIKRDLIDQKAQKINLIYPGVIDLIDVIENFIKDS", "aa_hexdigest": "4a2d0030fe739b402b27a8b5fe5cd040", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03838.20", "name": "RecU", "length": 60, "aa_cov": 0.8939393939393939, "hmm_cov": 1.0, "evalue": 6.232031524800981e-09, "score": 31.473615646362305, "start": 1, "stop": 59 } ], "seq_stats": { "molecular_weight": 7691.999399999998, "isoelectric_point": 6.533949089050293 }, "id": "APKECPOBEN_1155", "locus": "GCA002090215_01154" }, { "type": "cds", "sequence": "contig_1", "start": 650975, "stop": 651289, "strand": "-", "frame": 3, "gene": "hupA", "product": "putative MG353-like protein", "db_xrefs": [ "COG:B", "COG:COG0776", "GO:0003677", "GO:0030527", "SO:0001217", "UniRef:UniRef50_P47595", "UniRef:UniRef90_P47595" ], "nt": "ATGGAAAAAACAACAACATCGAGTAAGCCATTGTCTCGTAGCGAAATTAACAAGATTATTGCAGTAGCTACTGGAGTAAAAGAGGCTAAGATAAAAGAGATCTTTAAATACCTCAACACCCTCTTACTTAATGAATTAGTAAGTCGTAGCGTTTGCATTTTGCCCGAAAATTTGGGTAAATTACGCATTACGATTCGTAACGCTAGAATTCAAAAAGACATGAAAACTGGTGAAATGAAACACATTCCACCAAAACCATTAGTGCGTTACAGTCCAAGCAAAACCATTAAGGAAACGGCTGCTAAGGTACGCTGA", "aa": "MEKTTTSSKPLSRSEINKIIAVATGVKEAKIKEIFKYLNTLLLNELVSRSVCILPENLGKLRITIRNARIQKDMKTGEMKHIPPKPLVRYSPSKTIKETAAKVR", "aa_hexdigest": "3b0cdc9dd4db5fa729fc2ead1a810834", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47595", "query_cov": 1.0, "subject_cov": 0.9541284403669725, "identity": 0.9129999999999999, "score": 178.0, "evalue": 2.14e-56, "valid": true, "gene": "HupA", "product": "Uncharacterized protein MG353 homolog", "uniref50_id": "UniRef50_P47595", "cog_id": "COG0776", "cog_category": "B", "go_ids": [ "GO:0003677", "GO:0030527" ] }, "pscc": { "uniref50_id": "UniRef50_P47595", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47595" ], "product": "Uncharacterized protein MG353" }, "genes": [ "hupA" ], "id": "APKECPOBEN_1156", "locus": "GCA002090215_01155" }, { "type": "cds", "sequence": "contig_1", "start": 651438, "stop": 651848, "strand": "+", "frame": 3, "gene": null, "product": "putative MG354-like protein", "db_xrefs": [ "RefSeq:WP_010874887.1", "SO:0001217", "UniParc:UPI0000139690", "UniRef:UniRef100_P75248", "UniRef:UniRef50_P47596", "UniRef:UniRef90_P75248" ], "nt": "ATGGAACCAAATAATTTAAAAGAAGAGCTAGTTTCAGTCTTTGAAAAAGCTTGTTCTTCACATAAAGAAAGGCTTGATTTTATCTGTTCTGTAAGAGAAAGCGATACCTTTTCCAATGTGGATGTACCGCTAGCTCCTATTAAAACTATTATCGAAATTGCTAAAAACGAGGAAAATCAAACCGAAATTCTCAAATTAGCGATCGAAAACATTAAGACTCTATCAACTGTTGGGTCTGGTCAATACATTGCATCGCACTTCTCAACCCATAACGAAGTTGCTATTATTTTCTGTATCTCTTACTTTTTGTATCACTTCAACTTCTTGCACGATGAAAACAAAAAGCAGTTGCTAAAACGTGCCTTTGAAGCAGTTGCTGAAAAAATAGCTGACTACTTAAACGAAAACTAA", "aa": "MEPNNLKEELVSVFEKACSSHKERLDFICSVRESDTFSNVDVPLAPIKTIIEIAKNEENQTEILKLAIENIKTLSTVGSGQYIASHFSTHNEVAIIFCISYFLYHFNFLHDENKKQLLKRAFEAVAEKIADYLNEN", "aa_hexdigest": "3b4796a373f6743d4de66b1311841eeb", "start_type": "ATG", "rbs_motif": "AAAA", "ups": { "uniparc_id": "UPI0000139690", "ncbi_nrp_id": "WP_010874887.1", "uniref100_id": "UniRef100_P75248", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139690", "RefSeq:WP_010874887.1", "UniRef:UniRef100_P75248" ] }, "ips": { "uniref100_id": "UniRef100_P75248", "uniref90_id": "UniRef90_P75248" }, "psc": { "uniref90_id": "UniRef90_P75248", "product": "Uncharacterized protein MG354 homolog", "uniref50_id": "UniRef50_P47596" }, "pscc": { "uniref50_id": "UniRef50_P47596", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47596" ], "product": "Uncharacterized protein MG354" }, "genes": [], "id": "APKECPOBEN_1157", "locus": "GCA002090215_01156" }, { "type": "cds", "sequence": "contig_1", "start": 651845, "stop": 652969, "strand": "-", "frame": 3, "gene": "clpB", "product": "Chaperone protein ClpB", "db_xrefs": [ "COG:COG0542", "COG:O", "GO:0005524", "GO:0005737", "GO:0016887", "GO:0034605", "SO:0001217", "UniRef:UniRef50_P47597", "UniRef:UniRef90_P75247" ], "nt": "TTGCAAACACGCTTAGAGCTCTACCAAACGGAAGGTAACTATGAAGCTGCTTCTAAGATTCTTTACTATGACATCCCCAAACTTAAAAACCAATTAGAACAAGCACAGAAAAAGTATGTTGATTCCAAACATGACTTGTTTAAGACAGAAGTGTCAGAAAACGAAGTGGCCGAAGTAGTATCACAAGCTACCGGTATTCCCTTAAAGAAGCTGTTAGAAACCGAAAAGGAAAAGCTTTTGCATTTAGGTGATGAAATAAAAAAACGGGTTAAGGGTCAAGATGCGGCCGTGGAGACAGTGGTCAATACCGTTATGCGTGGAAGGGTAAACCTCAATGACCCTAACCGGCCAATTGGTTCCTTTATCTTTTTAGGCTCTACTGGTGTTGGTAAAACGGAATTGGCTAAATCACTAGCAGAGGTCTTGTTTGATAATGAAAAAGCGATGATTCGCTTTGACATGAGTGAATACATGGAAAAGCATTCGGTCGCTAAGTTAATCGGAGCGCCTCCTGGATATGTAGGTTATGAACAATCTGGTTTACTAACTGAAGCAGTCCGACGCAAACCTTACTGTGTGCTTCTGTTCGATGAAATTGAAAAAGCACACCCTGATGTAACTAATATTCTTTTACAAGTATTAGATGATGGTACTTTAAAGGACTCTCAAGGTCGTTTAGTTAACTTTAAGAATACGATGATAATTATGACTTCCAACTTGGGTTCCAATTACATTATGGAAAACAAACGCGATTTAGCGATGGAAGCACTAAAAAAACACTTCCGTGCTGAATTTATTAACCGGATTGATGAAATCGTTTTCTTTAGCGTATTGCAAAAAACAACCGTTTTAGAAATTATTACAAATTTATTGGATCAATTAAATCAAAGATTAGCTAAACAAAACTTAAAGTTCACGTTTGACCCTAAGTTAAATGAGTTTATTTACAAATCTAGTTTCGACGAACAGTTTGGTGCTCGACCAATTAAACGTTTTATCGATCGGCAAATAGCTACTCTAATTGCCAAGCAAATTCTGGAAGGTATTATTACTAAAGATGTTAGTTACAACGTAATAGTTGAGAAAGATAAAGTGGCAATAGTAGCCAATAAGGTTAAATCTTAG", "aa": "MQTRLELYQTEGNYEAASKILYYDIPKLKNQLEQAQKKYVDSKHDLFKTEVSENEVAEVVSQATGIPLKKLLETEKEKLLHLGDEIKKRVKGQDAAVETVVNTVMRGRVNLNDPNRPIGSFIFLGSTGVGKTELAKSLAEVLFDNEKAMIRFDMSEYMEKHSVAKLIGAPPGYVGYEQSGLLTEAVRRKPYCVLLFDEIEKAHPDVTNILLQVLDDGTLKDSQGRLVNFKNTMIIMTSNLGSNYIMENKRDLAMEALKKHFRAEFINRIDEIVFFSVLQKTTVLEIITNLLDQLNQRLAKQNLKFTFDPKLNEFIYKSSFDEQFGARPIKRFIDRQIATLIAKQILEGIITKDVSYNVIVEKDKVAIVANKVKS", "aa_hexdigest": "e8ac366f582e392158e748bda5bea9c0", "start_type": "TTG", "rbs_motif": "AAAA", "psc": { "uniref90_id": "UniRef90_UPI00258F0CA4", "query_cov": 0.9545454545454546, "subject_cov": 0.8419811320754716, "identity": 0.535, "score": 362.0, "evalue": 5.07e-120, "valid": false, "product": "AAA family ATPase", "uniref50_id": "UniRef50_A0A3D0HNG7" }, "genes": [ "clpB" ], "truncated": "3-prime", "pseudogene": { "start": 651845, "stop": 653269, "observations": { "mutation": [ 653029 ], "stop": [ 653029 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75247", "query_cov": 1.0, "subject_cov": 0.5223463687150838, "identity": 0.997, "score": 709.0, "evalue": 3.17e-252, "gene_start": 1, "gene_end": 374, "reference_start": 343, "reference_end": 716, "reference_sequence": "MDFSFTPTPDKRDFLKEMGRSINDEVLKNKVDPIIGRDNEIRRLIEILSRKNKNNPVLIGEPGVGKTAIVEGFVRRVVNNDVPLNLRDVEIYELSLSGLIAGTQYQGEFEKRVNGILKQVKESNGKIILFIDEIHQIVGLGRNXSSSGAMDIANILKPMLARGEIKVIGATTLKEYREYVEKDGALERRFQKILVSEPSQQEALTIMRGLKTRWELFHNLTIFDSALVAAVEMSARYIPDRNLPDKAIDLIDEASAKIKTEMASEPVVIDTLKREIINLETEYAALKQDKENADNKKKQGHLDNLKQQLDELKKKRDSLTAEWKKEKTNFESINKLKKEIEDLQTRLELYQTEGNYEAASKILYYDIPKLKNQLEQAQKKYVDSKHDLFKTEVSENEVAEVVSQATGIPLKKLLETEKEKLLHLGDEIKKRVKGQDAAVETVVNTVMRGRVNLNDPNRPIGSFIFLGSTGVGKTELAKSLAEVLFDNEKAMIRFDMSEYMEKHSVAKLIGAPPGYVGYEQSGLLTEAVRRKPYCVLLFDEIEKAHPDVTNILLQVLDDGTLKDSQGRLVNFKNTMIIMTSNLGSNYIMENKRDLAMEALKKHFRAEFINRIDEIVFFSVLQKTTVLEIITNLLDQLNQRLAKQNLKFTFDPKLNEFIYKSSFDEQFGARPIKRFIDRQIATLIAKQILEGIITKDVSYNVIVEKDKVAIVANKVKS" }, "paralog": false, "identity": 0.9978902953586498, "score": 912.0, "evalue": 0.0, "description": "Internal stop codon at 653029. Nonsense mutation around 653029.", "psc": { "uniref90_id": "UniRef90_P75247", "gene": "clpB", "product": "Chaperone protein ClpB", "uniref50_id": "UniRef50_P47597", "cog_id": "COG0542", "cog_category": "O", "go_ids": [ "GO:0005737", "GO:0005524", "GO:0016887", "GO:0034605" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75247", "UniRef:UniRef50_P47597", "COG:COG0542", "COG:O", "GO:0005737", "GO:0005524", "GO:0016887", "GO:0034605" ] }, "pscc": { "uniref50_id": "UniRef50_P47597", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47597" ], "product": "Chaperone protein ClpB" } }, "id": "APKECPOBEN_1158", "locus": "GCA002090215_01157" }, { "type": "cds", "sequence": "contig_1", "start": 653027, "stop": 653992, "strand": "-", "frame": 3, "gene": "clpB", "product": "Chaperone protein ClpB", "db_xrefs": [ "COG:COG0542", "COG:O", "GO:0005524", "GO:0005737", "GO:0016887", "GO:0034605", "SO:0001217", "UniRef:UniRef50_P47597", "UniRef:UniRef90_P75247" ], "nt": "ATGGATTTTAGTTTTACACCAACACCAGATAAGCGTGATTTCTTAAAGGAAATGGGGCGTAGCATTAACGATGAGGTGCTCAAAAATAAGGTTGATCCTATTATTGGTCGCGACAACGAAATTCGACGCTTAATTGAAATTTTAAGCCGGAAAAACAAGAACAATCCCGTTTTAATTGGTGAACCAGGCGTCGGGAAAACCGCCATTGTTGAAGGATTTGTAAGAAGGGTAGTTAATAATGACGTGCCTTTAAACTTGCGTGATGTTGAAATTTATGAACTTTCGTTGTCTGGTTTAATTGCTGGCACCCAATACCAAGGTGAGTTTGAAAAACGGGTTAACGGTATCTTAAAGCAAGTCAAAGAATCAAATGGCAAGATTATTTTATTCATTGATGAAATCCACCAAATAGTGGGGCTAGGTCGTAACTCCAGTAGTGGTGCCATGGACATTGCCAACATCTTAAAACCAATGTTAGCACGCGGTGAAATTAAGGTTATTGGTGCCACGACACTGAAAGAGTACCGTGAATATGTGGAGAAGGACGGCGCTTTAGAACGGCGCTTCCAGAAGATCCTTGTCAGTGAACCGAGTCAACAAGAAGCACTTACCATTATGCGGGGTTTGAAGACCCGTTGGGAACTTTTTCATAACCTCACCATCTTTGACAGTGCCTTGGTAGCTGCTGTAGAAATGTCAGCACGTTATATTCCTGATCGTAATTTACCCGATAAAGCGATTGATTTAATTGATGAAGCTAGTGCCAAGATTAAAACGGAAATGGCTTCAGAGCCAGTGGTAATTGACACCCTTAAGCGGGAAATTATTAACTTAGAAACTGAATATGCCGCTTTAAAACAAGATAAGGAAAATGCTGATAACAAGAAAAAACAAGGACACTTAGACAATCTAAAACAACAGTTAGATGAGTTAAAGAAAAAGCGTGATTCTCTAACTGCCGAATGA", "aa": "MDFSFTPTPDKRDFLKEMGRSINDEVLKNKVDPIIGRDNEIRRLIEILSRKNKNNPVLIGEPGVGKTAIVEGFVRRVVNNDVPLNLRDVEIYELSLSGLIAGTQYQGEFEKRVNGILKQVKESNGKIILFIDEIHQIVGLGRNSSSGAMDIANILKPMLARGEIKVIGATTLKEYREYVEKDGALERRFQKILVSEPSQQEALTIMRGLKTRWELFHNLTIFDSALVAAVEMSARYIPDRNLPDKAIDLIDEASAKIKTEMASEPVVIDTLKREIINLETEYAALKQDKENADNKKKQGHLDNLKQQLDELKKKRDSLTAE", "aa_hexdigest": "98b21fab96182594327a88b305f536c5", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_U4KPM2", "query_cov": 0.8535825545171339, "subject_cov": 0.9415807560137457, "identity": 0.588, "score": 314.0, "evalue": 1.1e-103, "valid": false, "product": "ATP-dependent Clp protease (ATP-binding subunit, clpB), 1", "uniref50_id": "UniRef50_A0A930RWV4" }, "genes": [ "clpB" ], "truncated": "5-prime", "pseudogene": { "start": 652724, "stop": 653992, "observations": { "mutation": [ 653029 ], "stop": [ 653029 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75247", "query_cov": 1.0031152647975077, "subject_cov": 0.44972067039106145, "identity": 0.997, "score": 603.0, "evalue": 2.02e-211, "gene_start": 1, "gene_end": 321, "reference_start": 1, "reference_end": 322, "reference_sequence": "MDFSFTPTPDKRDFLKEMGRSINDEVLKNKVDPIIGRDNEIRRLIEILSRKNKNNPVLIGEPGVGKTAIVEGFVRRVVNNDVPLNLRDVEIYELSLSGLIAGTQYQGEFEKRVNGILKQVKESNGKIILFIDEIHQIVGLGRNXSSSGAMDIANILKPMLARGEIKVIGATTLKEYREYVEKDGALERRFQKILVSEPSQQEALTIMRGLKTRWELFHNLTIFDSALVAAVEMSARYIPDRNLPDKAIDLIDEASAKIKTEMASEPVVIDTLKREIINLETEYAALKQDKENADNKKKQGHLDNLKQQLDELKKKRDSLTAEWKKEKTNFESINKLKKEIEDLQTRLELYQTEGNYEAASKILYYDIPKLKNQLEQAQKKYVDSKHDLFKTEVSENEVAEVVSQATGIPLKKLLETEKEKLLHLGDEIKKRVKGQDAAVETVVNTVMRGRVNLNDPNRPIGSFIFLGSTGVGKTELAKSLAEVLFDNEKAMIRFDMSEYMEKHSVAKLIGAPPGYVGYEQSGLLTEAVRRKPYCVLLFDEIEKAHPDVTNILLQVLDDGTLKDSQGRLVNFKNTMIIMTSNLGSNYIMENKRDLAMEALKKHFRAEFINRIDEIVFFSVLQKTTVLEIITNLLDQLNQRLAKQNLKFTFDPKLNEFIYKSSFDEQFGARPIKRFIDRQIATLIAKQILEGIITKDVSYNVIVEKDKVAIVANKVKS" }, "paralog": false, "identity": 0.9952718676122931, "score": 808.0, "evalue": 1.79e-294, "description": "Internal stop codon at 653029. Nonsense mutation around 653029.", "psc": { "uniref90_id": "UniRef90_P75247", "gene": "clpB", "product": "Chaperone protein ClpB", "uniref50_id": "UniRef50_P47597", "cog_id": "COG0542", "cog_category": "O", "go_ids": [ "GO:0005737", "GO:0005524", "GO:0016887", "GO:0034605" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75247", "UniRef:UniRef50_P47597", "COG:COG0542", "COG:O", "GO:0005737", "GO:0005524", "GO:0016887", "GO:0034605" ] }, "pscc": { "uniref50_id": "UniRef50_P47597", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47597" ], "product": "Chaperone protein ClpB" } }, "id": "APKECPOBEN_1159", "locus": "GCA002090215_01158" }, { "type": "cds", "sequence": "contig_1", "start": 654168, "stop": 654287, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCTCAAACACAGCAAATTATTGATTACAAAAAGTGGGTGCAAAAGCGCTTGAACTACTTTTTAAAGGCACTGTTCCCTAACACTTGGAAGATTGCCAAAACAATTCCGTTGTACTAG", "aa": "MPQTQQIIDYKKWVQKRLNYFLKALFPNTWKIAKTIPLY", "aa_hexdigest": "84e24c4ca8c6d5540c98f8f0f6fabad4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4827.7321, "isoelectric_point": 10.09466915130615 }, "id": "APKECPOBEN_1160", "locus": "GCA002090215_01159" }, { "type": "cds", "sequence": "contig_1", "start": 654297, "stop": 654569, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01633.27" ], "nt": "GTGGAATTAACAGATAAACACAAAGCGATTCCCAAAACCTTGTGTCACCACGATTCCACCTTTGATAATTTGGTTTATACCCCAAAAAAGCAGGTCGTTTTAATTGACTTTGAGTGGTCTTGTGTGGACAACCCTTACTATGAGATTGCCAACATTATTCGCGAAGAGTTGACAATTGAAACAGCCAACCAAATTATTGACTTGTACGGTAGCTTAGACAAAAAACTAGTTTTTGAAACGGTAATTTACGTGTTGCTGTTTGCCTTTCAGTGA", "aa": "MELTDKHKAIPKTLCHHDSTFDNLVYTPKKQVVLIDFEWSCVDNPYYEIANIIREELTIETANQIIDLYGSLDKKLVFETVIYVLLFAFQ", "aa_hexdigest": "a90ae292dc3c2d7c044b849a270f7ab7", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01633.27", "name": "Choline_kinase", "length": 47, "aa_cov": 0.5111111111111111, "hmm_cov": 1.0, "evalue": 4.535431534026695e-09, "score": 31.452850341796875, "start": 11, "stop": 56 } ], "seq_stats": { "molecular_weight": 10554.03859999999, "isoelectric_point": 4.813148689270019 }, "id": "APKECPOBEN_1161", "locus": "GCA002090215_01160" }, { "type": "cds", "sequence": "contig_1", "start": 654657, "stop": 655016, "strand": "-", "frame": 2, "gene": "cotS", "product": "putative MG356-like protein", "db_xrefs": [ "COG:COG0510", "COG:H", "SO:0001217", "UniRef:UniRef50_Q49423", "UniRef:UniRef90_P75246" ], "nt": "ATGAATAAGGGCGATGCAATTGCTTTTCTTTGTGAAAAGTTAAACCTCCAACCAACTGCTGTAGCTCATGTGGAACAAATCCACTCGGGTTTTACTAATTTTTCCTTTTTTACAGAACTGAAAGATGGGAGTAAGTACCAAGTCAGGTTAGCGCGTAAAGATGTACCGTTGAACCGACGCAACGAGCAAATTATCCTGGACTTAGTGACCCCCATCTTTGGTAACCCCTTTGTTTACTTTGATGTAACTGCCGGTAATGCCATTAAGAAGTGGATTGAAGGAAAACAACCAAAACGTCCCACAAGGCTGTTTTTAGAACAACTAGTGGCTTCTTTAAAAAAAGTACATGCAGTCGAGTGA", "aa": "MNKGDAIAFLCEKLNLQPTAVAHVEQIHSGFTNFSFFTELKDGSKYQVRLARKDVPLNRRNEQIILDLVTPIFGNPFVYFDVTAGNAIKKWIEGKQPKRPTRLFLEQLVASLKKVHAVE", "aa_hexdigest": "a4f1a497e500c1f96ff8828df40d2b1d", "start_type": "ATG", "rbs_motif": null, "genes": [ "cotS" ], "truncated": "5-prime", "pseudogene": { "start": 654354, "stop": 655016, "observations": { "mutation": [ 654635, 654659 ], "stop": [ 654635, 654659 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75246", "query_cov": 1.0, "subject_cov": 0.4219858156028369, "identity": 0.992, "score": 239.0, "evalue": 1.32e-77, "gene_start": 1, "gene_end": 119, "reference_start": 1, "reference_end": 119, "reference_sequence": "MNKGDAIDFLCEKLNLQPTAVAHVEQIHSGFTNFSFFTELKDGSKYQVRLARKDVPLNRRNEQIILDLVTPIFGNPFVYFDVTAGNAIKKWIEGKQPKRPTRLFLEQLVASLKKVHAVEWKQFANEIWIFDPLIYFNQTKLPDFYKKLYVELTDKHKAIPKTLCHHDSTFDNLVYTPKKQVVLIDFEWSCVDNPYYEIANIIREELTIETANQIIDLYGSLDKKLVFETVIYVLLFAFQWTEIMPQTQQIIDYKKWVQKRLNYFLKALFPNTWKIAKTIPLY" }, "paralog": false, "identity": 0.9863636363636363, "score": 440.0, "evalue": 2.79e-159, "description": "Internal stop codon at 654635, 654659. Nonsense mutation around 654635, 654659.", "psc": { "uniref90_id": "UniRef90_P75246", "gene": "CotS", "product": "Uncharacterized protein MG356 homolog", "uniref50_id": "UniRef50_Q49423", "cog_id": "COG0510", "cog_category": "H", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75246", "UniRef:UniRef50_Q49423", "COG:COG0510", "COG:H" ] }, "pscc": { "uniref50_id": "UniRef50_Q49423", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49423" ], "product": "Uncharacterized protein MG356" } }, "id": "APKECPOBEN_1162", "locus": "GCA002090215_01161" }, { "type": "cds", "sequence": "contig_1", "start": 655024, "stop": 656196, "strand": "-", "frame": 1, "gene": "ackA", "product": "Acetate kinase", "db_xrefs": [ "COG:C", "COG:COG0282", "EC:2.7.2.1", "GO:0000287", "GO:0005524", "GO:0005829", "GO:0006083", "GO:0006085", "GO:0008776", "KEGG:K00925", "RefSeq:WP_010874890.1", "SO:0001217", "UniParc:UPI0000125296", "UniRef:UniRef100_P75245", "UniRef:UniRef50_P75245", "UniRef:UniRef90_P75245" ], "nt": "ATGAACGACAACAAAATTTTAGTAGTCAATGCTGGCAGTAGCTCGATCAAATTCCAATTGTTTGATTATCACAAAAAAGTCCTAGCTAAAGCCCTTTGTGAACGAATTTTTGTCGATGGTTTTTTCAAACTTGAATTTAACGAACAGAAGGTTGAAGAAAAAGTAGCTTTCCCCGACCACCACGCCGCTGTAACCCACTTTTTAAACACACTTAAAAAGCACAAAATTATCCAGGAACTTAGTGACATTATTCTAGTTGGTCACCGCGTGGTGCAGGGCGCTAACTACTTTAAGGATTCGGTGATCGTAGATGCAGAAGCACTCGCTAAAATTAAGGAATTTATTAAGTTAGCGCCACTGCACAACAAGCCAGAGGCTGATGTGATTGAAATCTTTTTCAAAGAAGTTCCTAGTGCTAAGAATGTGGCAGTCTTTGACACAACCTTTCACACTACCATTCCCCAAGAAAACTACTTGTATGCGGTACCACGTTCATGGGAGCAAAAACACTTAGTACGTCGTTATGGCTTCCATGGTACTTCTTACAAGTTCATTAATAACTATTTAGAAAAGCACTTGAACAAGCAAAACCTGAACCTGATTGTGTGTCACTTGGGTAACGGTGCGAGTGTTTGTGCCATTAAGAATGGTAAGTCATTTAATACTTCAATGGGATTCACCCCGCTAGAAGGTCTTATTATGGGAACCCGTAGTGGTGACCTTGACCCTGCCATTATTGGTTATGTAGCAGAACAGGAAAACATGAGTGCTAGTGATGTGGTCAATGCGCTTAATAAAAAATCAGGGATGTTGGCGTTAACTGGTGCATCGGATATGCGTGATGTTTTCGCTAAACCCCAAGAAAACGCTGTGGCTATCAAAATGTATGTTAACCGGGTAGCGGATTACATTGCTAAATACTTGAACCAATTGGAAGGCAACATTGATGGTTTAGTGTTTACTGGTGGCATTGGCGAGAATGCTAGTGATTGTGTAGAGTTGTTTATTAACGCCGTTAAATCATTAGGCTTTGCGACTGATCTAAAACTGTTTGTCAAGTACGGTGATAGCTGTGTGGTATCCACGCCCCAAAGTAAGTACAAGATTTACCGGGTACGTACTAATGAAGAGTTAATGATTGTGGAAGACTCCATTCGACTAACTCAAAAATAG", "aa": "MNDNKILVVNAGSSSIKFQLFDYHKKVLAKALCERIFVDGFFKLEFNEQKVEEKVAFPDHHAAVTHFLNTLKKHKIIQELSDIILVGHRVVQGANYFKDSVIVDAEALAKIKEFIKLAPLHNKPEADVIEIFFKEVPSAKNVAVFDTTFHTTIPQENYLYAVPRSWEQKHLVRRYGFHGTSYKFINNYLEKHLNKQNLNLIVCHLGNGASVCAIKNGKSFNTSMGFTPLEGLIMGTRSGDLDPAIIGYVAEQENMSASDVVNALNKKSGMLALTGASDMRDVFAKPQENAVAIKMYVNRVADYIAKYLNQLEGNIDGLVFTGGIGENASDCVELFINAVKSLGFATDLKLFVKYGDSCVVSTPQSKYKIYRVRTNEELMIVEDSIRLTQK", "aa_hexdigest": "cc3a5769ac0214af4287f7e9e5d2356a", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000125296", "ncbi_nrp_id": "WP_010874890.1", "uniref100_id": "UniRef100_P75245", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000125296", "RefSeq:WP_010874890.1", "UniRef:UniRef100_P75245" ] }, "ips": { "uniref100_id": "UniRef100_P75245", "uniref90_id": "UniRef90_P75245" }, "psc": { "uniref90_id": "UniRef90_P75245", "gene": "ackA", "product": "Acetate kinase", "ec_ids": [ "2.7.2.1" ], "uniref50_id": "UniRef50_P75245", "cog_id": "COG0282", "cog_category": "C", "kegg_orthology_id": "K00925", "go_ids": [ "GO:0005829", "GO:0008776", "GO:0005524", "GO:0000287", "GO:0006083", "GO:0006085" ] }, "pscc": { "uniref50_id": "UniRef50_P75245", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75245" ], "product": "Acetate kinase" }, "genes": [ "ackA" ], "id": "APKECPOBEN_1163", "locus": "GCA002090215_01162" }, { "type": "cds", "sequence": "contig_1", "start": 656265, "stop": 656435, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAN4XFQ7", "UniRef:UniRef90_A0AAN4XFQ7" ], "nt": "ATGAGTGCTAAAACTTCATATATTGTGGCTGCTGTTTCGACAGCTGTAGGTATTATTCTCTACTTTATCTCTTCCCTCATGGGGGTAATTGGTGTAAGTAATGATAAGACCTTAATCATTGTCTTTTTTGTACTAGCTTCAATCATCATTATTAACGCCTGTGTTTTTTGA", "aa": "MSAKTSYIVAAVSTAVGIILYFISSLMGVIGVSNDKTLIIVFFVLASIIIINACVF", "aa_hexdigest": "83b14a31674ea43c546bcd3a635081ad", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0AAN4XFQ7", "query_cov": 1.0, "subject_cov": 0.8115942028985508, "identity": 1.0, "score": 94.4, "evalue": 2.28e-24, "valid": true, "uniref50_id": "UniRef50_A0AAN4XFQ7" }, "pscc": { "uniref50_id": "UniRef50_A0AAN4XFQ7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAN4XFQ7" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5899.115099999997, "isoelectric_point": 7.915057563781738 }, "id": "APKECPOBEN_1164", "locus": "GCA002090215_01163" }, { "type": "cds", "sequence": "contig_1", "start": 656537, "stop": 656959, "strand": "+", "frame": 2, "gene": null, "product": "putative protein MPN_534", "db_xrefs": [ "RefSeq:WP_010874891.1", "SO:0001217", "UniParc:UPI000013AD23", "UniRef:UniRef100_P75244", "UniRef:UniRef50_P75244", "UniRef:UniRef90_P75244" ], "nt": "ATGAATACTTTTAAAGAAACCCTCTTTTCATTAAAGAAAAGTAATTATTTGTTGAATGAGAGTGCTCAAATTCTCAATGATTGTGTAGTAAATAACAATGAAATCGATGAACGCTTACGCAATTCGATTAAATTTGCCCTTGGCATTATTAATTTAGCTAGTTACTGTCTTAAAAACGAACAGGAACAGCTAGACCATGAACTCAACCATGGTAATGAAGCGCCCTGGGAGTTTAGTTTCAATGAAGCTGAACAACTAATAGAATGCACTGTCAAAAATAACTTTGGCAACGAAAAACTAGTGGATTTAATTTTCCACATTGGTGATGCCATGGAGACTTACCGTACCACTAATATTAAGTTTAGAGTACCCAAGTCTTACTATGATGCCAAACAGCAAATTAGAAAGGTAATTAAAAACTAA", "aa": "MNTFKETLFSLKKSNYLLNESAQILNDCVVNNNEIDERLRNSIKFALGIINLASYCLKNEQEQLDHELNHGNEAPWEFSFNEAEQLIECTVKNNFGNEKLVDLIFHIGDAMETYRTTNIKFRVPKSYYDAKQQIRKVIKN", "aa_hexdigest": "19281e5bfc309493ec36856b74870de5", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013AD23", "ncbi_nrp_id": "WP_010874891.1", "uniref100_id": "UniRef100_P75244", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013AD23", "RefSeq:WP_010874891.1", "UniRef:UniRef100_P75244" ] }, "ips": { "uniref100_id": "UniRef100_P75244", "uniref90_id": "UniRef90_P75244" }, "psc": { "uniref90_id": "UniRef90_P75244", "product": "Uncharacterized protein MPN_534", "uniref50_id": "UniRef50_P75244" }, "pscc": { "uniref50_id": "UniRef50_P75244", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75244" ], "product": "Uncharacterized protein MPN_534" }, "genes": [], "id": "APKECPOBEN_1165", "locus": "GCA002090215_01164" }, { "type": "cds", "sequence": "contig_1", "start": 656965, "stop": 657585, "strand": "+", "frame": 1, "gene": "ruvA", "product": "Holliday junction branch migration protein RuvA", "db_xrefs": [ "COG:COG0632", "COG:L", "EC:3.6.4.12", "GO:0000400", "GO:0003678", "GO:0005524", "GO:0005737", "GO:0006281", "GO:0006310", "GO:0009378", "GO:0009379", "GO:0036121", "GO:0048476", "GO:0061749", "GO:1990518", "RefSeq:WP_010874892.1", "SO:0001217", "UniParc:UPI0000135351", "UniRef:UniRef100_P75243", "UniRef:UniRef50_Q49424", "UniRef:UniRef90_P75243" ], "nt": "ATGATTGCTTCAATTTTTGGAAAAATTACCTTTGTTGGTAAGCGAAAAATTATTGTTGAAGCCAATTGTATTTCCTATTGGTTTAATGTGAAAGAAAACCATAGCTTCGAAAAGAACTTGGAAAAACCGCGCCAGGTCTTCTGTCAGATCATTAAAAGAATGGTTACGAACCAGATCTTGGAAGAGGGCTTTGCCTTCAATACCTTAGAGGAAAAGGAATGGTTTTCGAAGTTCATCGAACTCAATGGCATTGGTAGTAAAACTGCTTTAAACTTGCTCAATAACAACCTCGAGGAAATGCGTGATTATATTAAAAACAGTAACTACCATGCCTTGACTAAGTTAACTGGTTCTAACAGTAAGGTAGCTCGCGCATTGCTGGCACTGGAACTGTATGACCGAGACGATGGTGGTAAACGGATTAAACCCAACACTGCCATGGCCAATGACTATGATGAGATGTTTGACACCTTAAAGTCGTTGGGCTATAAACCCCAAGATATCCAAAACGCTTTGAGCAAAATTGAAATTAAACCTAACTTTGATGTAAGTGAAGTGATTGCTGAAGTGATTAAGTTAATGTCTTTCCAGAACAATGAAGTTACAAATAAAACCGCCTAA", "aa": "MIASIFGKITFVGKRKIIVEANCISYWFNVKENHSFEKNLEKPRQVFCQIIKRMVTNQILEEGFAFNTLEEKEWFSKFIELNGIGSKTALNLLNNNLEEMRDYIKNSNYHALTKLTGSNSKVARALLALELYDRDDGGKRIKPNTAMANDYDEMFDTLKSLGYKPQDIQNALSKIEIKPNFDVSEVIAEVIKLMSFQNNEVTNKTA", "aa_hexdigest": "b50832c041587a46bea8bff4b6db39d6", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000135351", "ncbi_nrp_id": "WP_010874892.1", "uniref100_id": "UniRef100_P75243", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000135351", "RefSeq:WP_010874892.1", "UniRef:UniRef100_P75243" ] }, "ips": { "uniref100_id": "UniRef100_P75243", "uniref90_id": "UniRef90_P75243" }, "psc": { "uniref90_id": "UniRef90_P75243", "gene": "ruvA", "product": "Holliday junction branch migration protein RuvA", "ec_ids": [ "3.6.4.12" ], "uniref50_id": "UniRef50_Q49424", "cog_id": "COG0632", "cog_category": "L", "go_ids": [ "GO:0000400", "GO:0003678", "GO:0005524", "GO:0005737", "GO:0006281", "GO:0006310", "GO:0009378", "GO:0009379", "GO:0036121", "GO:0048476", "GO:0061749", "GO:1990518" ] }, "pscc": { "uniref50_id": "UniRef50_Q49424", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49424" ], "product": "Holliday junction branch migration complex subunit RuvA" }, "genes": [ "ruvA" ], "id": "APKECPOBEN_1166", "locus": "GCA002090215_01165" }, { "type": "cds", "sequence": "contig_1", "start": 657560, "stop": 657979, "strand": "+", "frame": 2, "gene": "ruvB", "product": "Holliday junction branch migration DNA helicase RuvB", "db_xrefs": [ "EC:3.6.4.12", "GO:0003677", "GO:0005524", "GO:0006281", "GO:0006310", "GO:0009378", "SO:0001217", "UniRef:UniRef50_UPI001EFC4F44", "UniRef:UniRef90_UPI001EFC4F44" ], "nt": "ATGAAGTTACAAATAAAACCGCCTAACAATTTCGCTGAGTTTGTCGGTAAACAAGAAATTATTAACCAAATTCAACTCAGCATTAAAGCGTCCCGTATCAATAAGGCGCAGTTGGACCACATTCTTTTATACGGTCCACCTGGTGTTGGTAAAACCACATTAGCTCGCTTAATTGCGAGTGAAATGAACACCAAACTGCAAATTATTCAGGGTGGGCACTTACAGCGACCGAGTGATTTTCTCAACGCCGTTTCCCTCATTAAAAAGGGTGATGTACTGTTTGTGGATGAAATCCATGCAGTAGCACCCAGTGTGATGGAGTTAATGTTTCCCGTGATGGATGATTTTCGCGTCCAAGTCTTAATTGGCAAGGACTTTAATTCCAAGATGGTGGAAATGAAAGTTAATCCGTTTACCTGA", "aa": "MKLQIKPPNNFAEFVGKQEIINQIQLSIKASRINKAQLDHILLYGPPGVGKTTLARLIASEMNTKLQIIQGGHLQRPSDFLNAVSLIKKGDVLFVDEIHAVAPSVMELMFPVMDDFRVQVLIGKDFNSKMVEMKVNPFT", "aa_hexdigest": "25c1121214f8dba1cd72b410497cfbed", "start_type": "ATG", "rbs_motif": null, "genes": [ "ruvB" ], "truncated": "3-prime", "pseudogene": { "start": 657560, "stop": 658256, "observations": { "insertion": [ 658232 ], "mutation": [ 657977 ], "stop": [ 657977 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001EFC4F44", "query_cov": 1.0, "subject_cov": 0.5965665236051502, "identity": 1.0, "score": 271.0, "evalue": 7.24e-91, "gene_start": 1, "gene_end": 139, "reference_start": 1, "reference_end": 139, "reference_sequence": "MKLQIKPPNNFAEFVGKQEIINQIQLSIKASRINKAQLDHILLYGPPGVGKTTLARLIASEMNTKLQIIQGGHLQRPSDFLNAVSLIKKGDVLFVDEIHAVAPSVMELMFPVMDDFRVQVLIGKDFNSKMVEMKVNPFTWIGATTQFGKIINPLEDRFGMILNIDYYSNQEIERIVSIYGEQMELELKPEEITQITQHSKQTPRIAIRIVKRLFEQKIVNKKIDLIWLLCSKA" }, "paralog": false, "identity": 0.9871244635193133, "score": 439.0, "evalue": 2.94e-159, "description": "Internal stop codon at 657977. Frameshift due to insertion around 658232. Nonsense mutation around 657977.", "psc": { "uniref90_id": "UniRef90_UPI001EFC4F44", "gene": "ruvB", "product": "Holliday junction branch migration DNA helicase RuvB", "ec_ids": [ "3.6.4.12" ], "uniref50_id": "UniRef50_UPI001EFC4F44", "go_ids": [ "GO:0003677", "GO:0005524", "GO:0006281", "GO:0006310", "GO:0009378" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001EFC4F44", "EC:3.6.4.12", "UniRef:UniRef50_UPI001EFC4F44", "GO:0003677", "GO:0005524", "GO:0006281", "GO:0006310", "GO:0009378" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001EFC4F44", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001EFC4F44" ], "product": "Holliday junction branch migration DNA helicase RuvB" } }, "id": "APKECPOBEN_1167", "locus": "GCA002090215_01166" }, { "type": "cds", "sequence": "contig_1", "start": 658037, "stop": 658483, "strand": "+", "frame": 2, "gene": "ruvB", "product": "Holliday junction branch migration DNA helicase RuvB", "db_xrefs": [ "COG:COG2255", "COG:L", "EC:3.6.4.-", "EC:3.6.4.12", "GO:0000400", "GO:0003677", "GO:0003689", "GO:0005524", "GO:0005737", "GO:0006281", "GO:0006310", "GO:0009378", "GO:0016887", "GO:0036121", "GO:0048476", "GO:0061749", "GO:0061775", "GO:0140584", "GO:0140665", "GO:0140849", "GO:1990518", "SO:0001217", "UniRef:UniRef50_Q49425", "UniRef:UniRef90_P75242" ], "nt": "ATGATCCTTAACATTGACTACTACAGTAACCAAGAGATCGAGCGGATTGTAAGCATCTATGGTGAGCAAATGGAGTTGGAATTAAAACCGGAAGAAATTACCCAGATTACCCAACACAGTAAGCAAACCCCGCGGATTGCCATTCGAATTGTCAAGCGTTTGTTTGAACAGAAAATAGTCAACAAAAAGATTGATCTGGCTGCTCTGTTCAAAAGCTTAATGATTTACAAAAATGGTTTACAGAGCATTGATGTGCAATATCTCAAAGCACTCAACGGGCAGTACGAGCCTCAGGGTATTAAGTCAATTTGTTCGATGCTAGGGATTGATAAATCCACTGTCGAAAACAAGATTGAGCCCTTCTTGCTGCGGGAAAACATGATCCAAAAGACCAAGAAAGGGCGGATCATAACCCGCACTGGGCGTAATTATTTAACTAGCTGCTAA", "aa": "MILNIDYYSNQEIERIVSIYGEQMELELKPEEITQITQHSKQTPRIAIRIVKRLFEQKIVNKKIDLAALFKSLMIYKNGLQSIDVQYLKALNGQYEPQGIKSICSMLGIDKSTVENKIEPFLLRENMIQKTKKGRIITRTGRNYLTSC", "aa_hexdigest": "c69cdcc102f00b3e67331262167c55dc", "start_type": "ATG", "rbs_motif": null, "genes": [ "ruvB" ], "truncated": "5-prime", "pseudogene": { "start": 657737, "stop": 658483, "observations": { "mutation": [ 657977 ], "stop": [ 657977 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75242", "query_cov": 1.0, "subject_cov": 0.4820846905537459, "identity": 1.0, "score": 284.0, "evalue": 1.16e-94, "gene_start": 1, "gene_end": 148, "reference_start": 160, "reference_end": 307, "reference_sequence": "MKLQIKPPNNFAEFVGKQEIINQIQLSIKASRINKAQLDHILLYGPPGVGKTTLARLIASEMNTKLQIIQGGHLQRPSDFLNAVSLIKKGDVLFVDEIHAVAPSVMELMFPVMDDFRVQVLIGKDFNSKMVEMKVNPFTWIGATTQFGKIINPLEDRFGMILNIDYYSNQEIERIVSIYGEQMELELKPEEITQITQHSKQTPRIAIRIVKRLFEQKIVNKKIDLAALFKSLMIYKNGLQSIDVQYLKALNGQYEPQGIKSICSMLGIDKSTVENKIEPFLLRENMIQKTKKGRIITRTGRNYLTSC" }, "paralog": false, "identity": 0.9959677419354839, "score": 482.0, "evalue": 5.45e-175, "description": "Internal stop codon at 657977. Nonsense mutation around 657977.", "psc": { "uniref90_id": "UniRef90_P75242", "gene": "ruvB", "product": "Holliday junction branch migration DNA helicase RuvB", "ec_ids": [ "3.6.4.-", "3.6.4.12" ], "uniref50_id": "UniRef50_Q49425", "cog_id": "COG2255", "cog_category": "L", "go_ids": [ "GO:0000400", "GO:0003677", "GO:0003689", "GO:0005524", "GO:0005737", "GO:0006281", "GO:0006310", "GO:0009378", "GO:0016887", "GO:0036121", "GO:0048476", "GO:0061749", "GO:0061775", "GO:0140584", "GO:0140665", "GO:0140849", "GO:1990518" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75242", "EC:3.6.4.-", "EC:3.6.4.12", "UniRef:UniRef50_Q49425", "COG:COG2255", "COG:L", "GO:0000400", "GO:0003677", "GO:0003689", "GO:0005524", "GO:0005737", "GO:0006281", "GO:0006310", "GO:0009378", "GO:0016887", "GO:0036121", "GO:0048476", "GO:0061749", "GO:0061775", "GO:0140584", "GO:0140665", "GO:0140849", "GO:1990518" ] }, "pscc": { "uniref50_id": "UniRef50_Q49425", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49425" ], "product": "Holliday junction branch migration complex subunit RuvB" } }, "id": "APKECPOBEN_1168", "locus": "GCA002090215_01167" }, { "type": "cds", "sequence": "contig_1", "start": 658469, "stop": 659131, "strand": "-", "frame": 3, "gene": "dinP", "product": "putative MG360-like protein", "db_xrefs": [ "COG:COG0389", "COG:L", "GO:0003684", "GO:0003887", "GO:0005829", "GO:0009432", "GO:0042276", "SO:0001217", "UniRef:UniRef50_P75241", "UniRef:UniRef90_P75241" ], "nt": "GTGGAACTTCCTGGGATAGGCAAACGACAACTTGACAACGCCTTTAAAAATAATTTTCACAAGATTGGAGACCTCGCTAAGTGTAAAGATGTGACCTTGTTCAAACGGGTGTTTGGTATTGCGTGGGAAAGCTTGCACGCTGTGGCTTTGGGTGAAACTTATACGCAAAGCGAGCAGGATGTCAAAAGTCGCTCAATTGCTGTAAGTGAAACGCTGGAAGATTTGAACTACTCTTCCAATCAACTCCAGCAAAAACTCACTAGTATTTTCAACGAGTTGTATGCTAGGTTACAACTGTCCTTTCAAATGTGCAAGGGAGTAGTGGTGCAGTTAAAGAGCAATGACTTTATTGTCAACTCCCACTCTCAATCGATCAAGAAATACACCGCTGATTACCAAACCCTACTCGTAATAGTCAAAAAGCTCTTTAACAGGCTATTAATGGGTGTGGGGCTTAACATCCGCTTAATTGGCGTGAGTTTTTTTGGATTAAAAAATAACCCCTCCAGTTCAAGACCAGAAGGGTTACTTTTTTATGAGTATCAGCAAGCTAAGCCTAAACAACAGACTGCTCACTTTGCTTTAGATCAAATGATCTATGAGATTAACCAAAGCTTTGGTTATGAGATTATCCAGCGTGCCAAAAAGTTAGCAGCTAGTTAA", "aa": "MELPGIGKRQLDNAFKNNFHKIGDLAKCKDVTLFKRVFGIAWESLHAVALGETYTQSEQDVKSRSIAVSETLEDLNYSSNQLQQKLTSIFNELYARLQLSFQMCKGVVVQLKSNDFIVNSHSQSIKKYTADYQTLLVIVKKLFNRLLMGVGLNIRLIGVSFFGLKNNPSSSRPEGLLFYEYQQAKPKQQTAHFALDQMIYEINQSFGYEIIQRAKKLAAS", "aa_hexdigest": "90bf47b9eb9554f52e98fc31ac938fa3", "start_type": "GTG", "rbs_motif": null, "genes": [ "dinP" ], "truncated": "3-prime", "pseudogene": { "start": 658469, "stop": 659431, "observations": { "mutation": [ 659146 ], "stop": [ 659146 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75241", "query_cov": 1.0, "subject_cov": 0.5339805825242718, "identity": 0.991, "score": 422.0, "evalue": 2.87e-146, "gene_start": 1, "gene_end": 220, "reference_start": 193, "reference_end": 412, "reference_sequence": "MIGTFTYLDPTIQADPNLLFFYFDFDAFFASVEEIENPELKNQPLIVGNRTSRSVVSTCNYLARSYGIKSGMPIAKALELCPQAIFATSHFRNYRKYSAKIFAMIAEQFNLEVHTLSIDEGFVCFRDLSPRKAFSLAKRIQRHVYEQLNFHISIGISNQFTLAKIFSNQAKPFGVKSCFSKEVKRKLWPLPIVELPGIGKRQLDNAFKNNFHKIGDLAKCKDVTLFKRVFGIAWESLHAVALGETYTQSEQDVKSRSIAVSETLEYLNYSSNQLQQKLTSIFNELYARLQLSFQMCKGVVVQLKSNDFIVNSHSQSIKKYTADYQTLLVIVKKLFNRLLMGVGLNIRLIGVSFFGLKNNPSSSRPEGLLFYEYQQAKPKQQTAHFALDQMIYEINQSFGYEIIQRAKKLAAS" }, "paralog": false, "identity": 0.99375, "score": 623.0, "evalue": 1.16e-227, "description": "Internal stop codon at 659146. Nonsense mutation around 659146.", "psc": { "uniref90_id": "UniRef90_P75241", "gene": "DinP", "product": "Uncharacterized protein MG360 homolog", "uniref50_id": "UniRef50_P75241", "cog_id": "COG0389", "cog_category": "L", "go_ids": [ "GO:0005829", "GO:0003684", "GO:0003887", "GO:0042276", "GO:0009432" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75241", "UniRef:UniRef50_P75241", "COG:COG0389", "COG:L", "GO:0005829", "GO:0003684", "GO:0003887", "GO:0042276", "GO:0009432" ] }, "pscc": { "uniref50_id": "UniRef50_P75241", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75241" ], "product": "Uncharacterized protein MG360 homolog" } }, "id": "APKECPOBEN_1169", "locus": "GCA002090215_01168" }, { "type": "cds", "sequence": "contig_1", "start": 659144, "stop": 659707, "strand": "-", "frame": 3, "gene": "dinP", "product": "putative MG360-like protein", "db_xrefs": [ "COG:COG0389", "COG:L", "GO:0003684", "GO:0003887", "GO:0005829", "GO:0009432", "GO:0042276", "SO:0001217", "UniRef:UniRef50_P75241", "UniRef:UniRef90_P75241" ], "nt": "ATGATCGGCACTTTTACATATTTAGATCCAACCATACAAGCTGATCCTAATCTGCTTTTTTTCTATTTTGATTTTGATGCCTTTTTTGCATCAGTAGAAGAGATAGAAAACCCCGAGCTCAAAAATCAACCTTTAATTGTCGGTAACCGGACATCTAGGAGTGTGGTGTCGACTTGTAATTACCTTGCCCGGAGCTATGGCATTAAGTCGGGCATGCCAATTGCTAAGGCCTTGGAATTGTGTCCCCAAGCGATCTTTGCCACATCACACTTTCGTAACTACCGCAAGTATTCCGCTAAGATCTTTGCCATGATTGCTGAGCAGTTTAACCTAGAGGTACACACCCTTTCGATTGATGAGGGCTTTGTGTGTTTTCGTGACCTTTCACCCCGTAAAGCATTTAGTTTGGCTAAACGGATTCAGCGCCACGTTTATGAACAGTTAAACTTTCACATCTCCATTGGTATTTCCAACCAATTTACCCTAGCGAAAATCTTTTCCAATCAGGCTAAACCGTTTGGGGTTAAAAGCTGTTTTAGCAAGGAAGTTAAACGCAAACTCTGA", "aa": "MIGTFTYLDPTIQADPNLLFFYFDFDAFFASVEEIENPELKNQPLIVGNRTSRSVVSTCNYLARSYGIKSGMPIAKALELCPQAIFATSHFRNYRKYSAKIFAMIAEQFNLEVHTLSIDEGFVCFRDLSPRKAFSLAKRIQRHVYEQLNFHISIGISNQFTLAKIFSNQAKPFGVKSCFSKEVKRKL", "aa_hexdigest": "ef81b4fc58cc981941b32eeebdbd9fa7", "start_type": "ATG", "rbs_motif": "AATAA", "genes": [ "dinP" ], "truncated": "5-prime", "pseudogene": { "start": 658841, "stop": 659707, "observations": { "mutation": [ 659146 ], "stop": [ 659146 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75241", "query_cov": 1.0, "subject_cov": 0.4538834951456311, "identity": 1.0, "score": 374.0, "evalue": 1.04e-127, "gene_start": 1, "gene_end": 187, "reference_start": 1, "reference_end": 187, "reference_sequence": "MIGTFTYLDPTIQADPNLLFFYFDFDAFFASVEEIENPELKNQPLIVGNRTSRSVVSTCNYLARSYGIKSGMPIAKALELCPQAIFATSHFRNYRKYSAKIFAMIAEQFNLEVHTLSIDEGFVCFRDLSPRKAFSLAKRIQRHVYEQLNFHISIGISNQFTLAKIFSNQAKPFGVKSCFSKEVKRKLWPLPIVELPGIGKRQLDNAFKNNFHKIGDLAKCKDVTLFKRVFGIAWESLHAVALGETYTQSEQDVKSRSIAVSETLEYLNYSSNQLQQKLTSIFNELYARLQLSFQMCKGVVVQLKSNDFIVNSHSQSIKKYTADYQTLLVIVKKLFNRLLMGVGLNIRLIGVSFFGLKNNPSSSRPEGLLFYEYQQAKPKQQTAHFALDQMIYEINQSFGYEIIQRAKKLAAS" }, "paralog": false, "identity": 0.9930555555555556, "score": 567.0, "evalue": 2.61e-206, "description": "Internal stop codon at 659146. Nonsense mutation around 659146.", "psc": { "uniref90_id": "UniRef90_P75241", "gene": "DinP", "product": "Uncharacterized protein MG360 homolog", "uniref50_id": "UniRef50_P75241", "cog_id": "COG0389", "cog_category": "L", "go_ids": [ "GO:0005829", "GO:0003684", "GO:0003887", "GO:0042276", "GO:0009432" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75241", "UniRef:UniRef50_P75241", "COG:COG0389", "COG:L", "GO:0005829", "GO:0003684", "GO:0003887", "GO:0042276", "GO:0009432" ] }, "pscc": { "uniref50_id": "UniRef50_P75241", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75241" ], "product": "Uncharacterized protein MG360 homolog" } }, "id": "APKECPOBEN_1170", "locus": "GCA002090215_01169" }, { "type": "cds", "sequence": "contig_1", "start": 659825, "stop": 660310, "strand": "+", "frame": 2, "gene": "rplJ", "product": "50S ribosomal protein L10", "db_xrefs": [ "COG:COG0244", "COG:J", "GO:0003735", "GO:0006412", "GO:0015934", "GO:0070180", "KEGG:K02864", "RefSeq:WP_010874895.1", "SO:0001217", "UniParc:UPI0000133B3C", "UniRef:UniRef100_P75240", "UniRef:UniRef50_P36263", "UniRef:UniRef90_P75240" ], "nt": "ATGGAAGCTAAAAAAGATAAAGCTCAGCAGGTTGCTGATGTCTCCCATTTGCTTTCAACCAGTGCAGGATTTGTCATTTTTGACTATACCAGCATGTCAGCAATTGAAGCGACTTCTATTCGCAAGAAGTTGTTTAAGAATGGCAGCAAAATTAAGGTAATTAAAAACAATATTTTGCGCCGTGCTTTAAAAGCTGGTAAGTTTGAAGGAATTGACGAAACTGCCATTAAAGGCAAATTGGCGGTTGCTGTTGGAGTGAACGAAATTGTAGAAACGTTGAAAGCTGTTGACGGTGTGGTAAAGGCGAAGGAAGCGATGAACTTTGTTTGTGGTTACTTTGACAACCGTGCTTTTAACAGTGCTGATTTAGAGAAGATTGCAAAACTGCCAGGACGAAACGAACTTTATGGGATGTTTCTCTCGGTATTACAAGCTCCATTGCGCAAGTTCTTATACGCTTTGGAAGCAGTTAAGGCTGCAAAATAA", "aa": "MEAKKDKAQQVADVSHLLSTSAGFVIFDYTSMSAIEATSIRKKLFKNGSKIKVIKNNILRRALKAGKFEGIDETAIKGKLAVAVGVNEIVETLKAVDGVVKAKEAMNFVCGYFDNRAFNSADLEKIAKLPGRNELYGMFLSVLQAPLRKFLYALEAVKAAK", "aa_hexdigest": "82648b8a97d4dd48affe0eb54c8d0619", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000133B3C", "ncbi_nrp_id": "WP_010874895.1", "uniref100_id": "UniRef100_P75240", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000133B3C", "RefSeq:WP_010874895.1", "UniRef:UniRef100_P75240" ] }, "ips": { "uniref100_id": "UniRef100_P75240", "uniref90_id": "UniRef90_P75240" }, "psc": { "uniref90_id": "UniRef90_P75240", "gene": "rplJ", "product": "50S ribosomal protein L10", "uniref50_id": "UniRef50_P36263", "cog_id": "COG0244", "cog_category": "J", "kegg_orthology_id": "K02864", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015934", "GO:0070180" ] }, "pscc": { "uniref50_id": "UniRef50_P36263", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P36263" ], "product": "Large ribosomal subunit protein uL10" }, "genes": [ "rplJ" ], "id": "APKECPOBEN_1171", "locus": "GCA002090215_01170" }, { "type": "cds", "sequence": "contig_1", "start": 660348, "stop": 660716, "strand": "+", "frame": 3, "gene": "rplL", "product": "50S ribosomal protein L7/L12", "db_xrefs": [ "COG:COG0222", "COG:J", "GO:0003729", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625", "RefSeq:WP_010874896.1", "SO:0001217", "UniParc:UPI00001341B5", "UniRef:UniRef100_P75239", "UniRef:UniRef50_P36255", "UniRef:UniRef90_P36255" ], "nt": "ATGGCAAAACTAGATAAAAACCAATTAATTGAATCGTTGAAGGAAATGACCATCATGGAAATCGATGAAATCATTAAGGCTGTAGAAGAAGCTTTTGGAGTATCGGCAACACCTGTAGTAGCTGCTGGTGCTGTTGGTGGTACACAAGAAGCTGCTAGCGAAGTGACTGTGAAAGTTACTGGTTACACTGACAACGCTAAATTAGCTGTGTTAAAGCTTTACCGCGAAATTGCTGGTGTTGGTTTAATGGAAGCTAAAACTGCTGTGGAAAAACTTCCTTGTGTTGTTAAGCAAGACATCAAACCTGAAGAAGCTGAAGAACTTAAAAAGCGTTTCGTTGAAGTTGGAGCAACTGTTGAAATCAAATAA", "aa": "MAKLDKNQLIESLKEMTIMEIDEIIKAVEEAFGVSATPVVAAGAVGGTQEAASEVTVKVTGYTDNAKLAVLKLYREIAGVGLMEAKTAVEKLPCVVKQDIKPEEAEELKKRFVEVGATVEIK", "aa_hexdigest": "b467230a3713ef0da97269c657dbc075", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001341B5", "ncbi_nrp_id": "WP_010874896.1", "uniref100_id": "UniRef100_P75239", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001341B5", "RefSeq:WP_010874896.1", "UniRef:UniRef100_P75239" ] }, "ips": { "uniref100_id": "UniRef100_P75239", "uniref90_id": "UniRef90_P36255" }, "psc": { "uniref90_id": "UniRef90_P36255", "gene": "rplL", "product": "50S ribosomal protein L7/L12", "uniref50_id": "UniRef50_P36255", "cog_id": "COG0222", "cog_category": "J", "go_ids": [ "GO:0003729", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_P36255", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P36255" ], "product": "Large ribosomal subunit protein bL12" }, "genes": [ "rplL" ], "id": "APKECPOBEN_1172", "locus": "GCA002090215_01171" }, { "type": "cds", "sequence": "contig_1", "start": 660723, "stop": 660896, "strand": "+", "frame": 3, "gene": "rpmF", "product": "50S ribosomal protein L32", "db_xrefs": [ "COG:COG0333", "COG:J", "GO:0003735", "GO:0006412", "GO:0015934", "RefSeq:WP_010874897.1", "SO:0001217", "UniParc:UPI0000165F9C", "UniRef:UniRef100_P75238", "UniRef:UniRef50_P47603", "UniRef:UniRef90_P75238" ], "nt": "ATGGCAGTACAACAACGACGCTCAAGCAAACACCGTCGTGACAAAAGACGTTCCCACGATGCACTAACAGCTCAAGCGCTGAGTGTTTGCCAAAAATGCGGTAAGAAAAAACTGTTCCACCGAGTCTGTTCGTGTGGAATGTATGGTGATCTGAGAGTAAAAAAGGCTTATTAA", "aa": "MAVQQRRSSKHRRDKRRSHDALTAQALSVCQKCGKKKLFHRVCSCGMYGDLRVKKAY", "aa_hexdigest": "a5783c96b7f62d63cbb77818d06c12b3", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000165F9C", "ncbi_nrp_id": "WP_010874897.1", "uniref100_id": "UniRef100_P75238", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000165F9C", "RefSeq:WP_010874897.1", "UniRef:UniRef100_P75238" ] }, "ips": { "uniref100_id": "UniRef100_P75238", "uniref90_id": "UniRef90_P75238" }, "psc": { "uniref90_id": "UniRef90_P75238", "gene": "rpmF", "product": "50S ribosomal protein L32", "uniref50_id": "UniRef50_P47603", "cog_id": "COG0333", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015934" ] }, "pscc": { "uniref50_id": "UniRef50_P47603", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47603" ], "product": "Large ribosomal subunit protein bL32" }, "genes": [ "rpmF" ], "id": "APKECPOBEN_1173", "locus": "GCA002090215_01172" }, { "type": "cds", "sequence": "contig_1", "start": 660906, "stop": 661169, "strand": "-", "frame": 2, "gene": "rpsT", "product": "30S ribosomal protein S20", "db_xrefs": [ "COG:COG0268", "COG:J", "GO:0003723", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904", "KEGG:K02968", "RefSeq:WP_010874898.1", "SO:0001217", "UniParc:UPI0000134E4B", "UniRef:UniRef100_P75237", "UniRef:UniRef50_P55750", "UniRef:UniRef90_P75237" ], "nt": "ATGGCTAATATCAAATCGAACGAAAAACGTTTACGACAAAACATTAAACGTAATCTGAATAACAAGGGACAGAAAACTAAGTTAAAGACCAACGTGAAGAATTTTCACAAGGAAATTAACCTAGACAACCTTGGTAATGTTTATTCCCAAGCAGATCGCTTAGCACGTAAAGGTATTATTTCTACTAATCGTGCGCGTCGTTTAAAATCACGCAACGTTGCTGTTTTAAATAAAACACAAGTAACAGCTGTTGAAGGCAAATAA", "aa": "MANIKSNEKRLRQNIKRNLNNKGQKTKLKTNVKNFHKEINLDNLGNVYSQADRLARKGIISTNRARRLKSRNVAVLNKTQVTAVEGK", "aa_hexdigest": "538c8e0f9db797a8beca525fe42905be", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000134E4B", "ncbi_nrp_id": "WP_010874898.1", "uniref100_id": "UniRef100_P75237", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000134E4B", "RefSeq:WP_010874898.1", "UniRef:UniRef100_P75237" ] }, "ips": { "uniref100_id": "UniRef100_P75237", "uniref90_id": "UniRef90_P75237" }, "psc": { "uniref90_id": "UniRef90_P75237", "gene": "rpsT", "product": "30S ribosomal protein S20", "uniref50_id": "UniRef50_P55750", "cog_id": "COG0268", "cog_category": "J", "kegg_orthology_id": "K02968", "go_ids": [ "GO:0003723", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_P55750", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P55750" ], "product": "Small ribosomal subunit protein bS20" }, "genes": [ "rpsT" ], "id": "APKECPOBEN_1174", "locus": "GCA002090215_01173" }, { "type": "cds", "sequence": "contig_1", "start": 661230, "stop": 661388, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGGTGGTGGACAACAGGGTGGTTTTTTTGGCCTACTCGTCATAATTATCCCTGTCATATTGTTGATTGTCTTTTTCTCTAAAAAGAAGAATTCACAAAAAACAGAGTTTGGTGGTGAAGGGGGCAGCCGTGCTTCCAAAAAAGATGAAGTTTGA", "aa": "MNGGGQQGGFFGLLVIIIPVILLIVFFSKKKNSQKTEFGGEGGSRASKKDEV", "aa_hexdigest": "b3b6d63816216fe6ea79769fefa8c627", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5528.383600000002, "isoelectric_point": 9.69799518585205 }, "id": "APKECPOBEN_1175", "locus": "GCA002090215_01174" }, { "type": "cds", "sequence": "contig_1", "start": 661473, "stop": 661886, "strand": "+", "frame": 3, "gene": null, "product": "putative MG364-like protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47604", "UniRef:UniRef90_P75236" ], "nt": "TTGCACTCCAGGAAAGACCGGCAGTTTTTTAACCAAGAAGTGCAAGCTTACATTACAGCCCATAACTTAAGCAAAACAGCAGCCAAGCGTTACCGTCACGAGCAATTAAAACTCAAACAACGTGAACTGTACTGCATTTACTTCATTACCAAGGACGCTAAAACCAGCGTTTTTGATGAAGCACGCATTATTGAAGCTGAAGTTTACCAAAAGCCCAATAAAACGGGTAAGGGTGCACCAGAACGTTTAATTCGAATCTTAGGATTGAAAAACTTCAATGACGAAATGAAGTGGATTCAACCACTAATGGATCGCGAGGAAAAACGAAAGGAAAAAGAAGAGAAACGCAAGCGGGAATTGGCAGCACGTCAGTTAAAAAGACAAGAAAAGAAAAAACAAAAAACTTCCAAATAA", "aa": "MHSRKDRQFFNQEVQAYITAHNLSKTAAKRYRHEQLKLKQRELYCIYFITKDAKTSVFDEARIIEAEVYQKPNKTGKGAPERLIRILGLKNFNDEMKWIQPLMDREEKRKEKEEKRKRELAARQLKRQEKKKQKTSK", "aa_hexdigest": "be6a29ee600b559f269b992cea5c13c5", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 661329, "stop": 661886, "observations": { "mutation": [ 661386 ], "stop": [ 661386 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75236", "query_cov": 1.0, "subject_cov": 0.6284403669724771, "identity": 0.993, "score": 233.0, "evalue": 9.5e-76, "gene_start": 1, "gene_end": 137, "reference_start": 82, "reference_end": 218, "reference_sequence": "MNGGGQQGGFFGLLVIIIPVILLIVFFSKKKNSQKTEFGGEGGSRASKKDEVWKTVKQFLQEQNERGKEIIKTFVAKNPNPLHSRKDRQFFNQEVQAYITAHNLSKTAAKRYRHEQLKLKQRELYCIYFITKDAKTSVFDEARIIEAEVYQKPNKTGKGAPERLIRILGLKNFNDEMKWIQPLMDREEKRKEKEEKRKRELAARQLKRQEKKKQKTSK" }, "paralog": false, "identity": 0.9837837837837838, "score": 327.0, "evalue": 2.85e-115, "description": "Internal stop codon at 661386. Nonsense mutation around 661386.", "psc": { "uniref90_id": "UniRef90_P75236", "product": "Uncharacterized protein MG364 homolog", "uniref50_id": "UniRef50_P47604", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75236", "UniRef:UniRef50_P47604" ] }, "pscc": { "uniref50_id": "UniRef50_P47604", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47604" ], "product": "Uncharacterized protein MG364" } }, "id": "APKECPOBEN_1176", "locus": "GCA002090215_01175" }, { "type": "cds", "sequence": "contig_1", "start": 661891, "stop": 662256, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00551.24" ], "nt": "ATGATTAAAGTGGTTTTCTTTGGGACATCCACCCTTTCAAAGTGTTGTTTAGAAGCAATTTTTCAAGATCCTGAGTTTGTGGTTTGCGGCGTAGTGACCCAGCCCGATAAGGTAAACGAGCGCAACAATAAAATTAACTTTTCTGCAGTTAAACAATTTTGTATAGAAAACAACATTCCCTGTTTTCAACCAGAAAAAAACATTCAGATTAAAACAGAGTTAGCCCAACTCCAAGCTGATATTGGCGTTTGTGTAGCCTTTGGCCAATACATCCACAACGATATTATTAACCTCTTTCCTTACAAGATTGCCAACCTACACCCCTCAAAATTACCACTACTGCGTGGTGGAGCACCATTGCACTGA", "aa": "MIKVVFFGTSTLSKCCLEAIFQDPEFVVCGVVTQPDKVNERNNKINFSAVKQFCIENNIPCFQPEKNIQIKTELAQLQADIGVCVAFGQYIHNDIINLFPYKIANLHPSKLPLLRGGAPLH", "aa_hexdigest": "384d25ffad9edeb7870b72daad1e208b", "start_type": "ATG", "rbs_motif": "AAAA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00551.24", "name": "Formyl_trans_N", "length": 123, "aa_cov": 0.9752066115702479, "hmm_cov": 0.975609756097561, "evalue": 6.589945321447271e-16, "score": 54.031036376953125, "start": 3, "stop": 120 } ], "seq_stats": { "molecular_weight": 13547.68919999998, "isoelectric_point": 7.602692604064942 }, "id": "APKECPOBEN_1177", "locus": "GCA002090215_01176" }, { "type": "cds", "sequence": "contig_1", "start": 662644, "stop": 662826, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02911.23" ], "nt": "ATGACAAGTACAACCGCAGTTGGTCAAATCACTAAAATTTCTAAACAAGGTATAGAAATCGCCTTAGCTAATGATGAAATTGTTCTACTCCAAATAATTCAAATTCCGGGAAAGCGGGCAATGGGAGTGTCAGAAATAATTAACGGTAAACATCCTTTTGCTGAAGGTAAATTTTTTCATTAA", "aa": "MTSTTAVGQITKISKQGIEIALANDEIVLLQIIQIPGKRAMGVSEIINGKHPFAEGKFFH", "aa_hexdigest": "19dbfaca56bd86a3e6a12aada593cd65", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02911.23", "name": "Formyl_trans_C", "length": 44, "aa_cov": 0.7333333333333333, "hmm_cov": 0.9772727272727273, "evalue": 3.440697089959477e-08, "score": 29.089759826660156, "start": 7, "stop": 50 } ], "seq_stats": { "molecular_weight": 6491.536899999999, "isoelectric_point": 8.18195667266846 }, "id": "APKECPOBEN_1178", "locus": "GCA002090215_01177" }, { "type": "cds", "sequence": "contig_1", "start": 662826, "stop": 663950, "strand": "+", "frame": 3, "gene": null, "product": "putative MG366-like protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47606", "UniRef:UniRef50_UPI00031D7E73", "UniRef:UniRef90_P75234" ], "nt": "ATGCTCACCAAGGCCTTTTTTTTAAAAAACTTTGATCGCTCAAAGGAGTTGATTCCTCTGTCTTACGCTGATGTTTTTGGCGCTGCTAGTCAATTGTTAAAAAAGCACCATAAGCAGGTCGATACCGAAATTGATGTGCAAGAAGATCTAATTGAAGATCCGGTATTTGAAATAGATATTTTAGAGCTACTCAACGAAGCGCCAGAACTATTATTTGATAGTAAAAATCCTCGGGTTAAAGAGGCACAAATCATTATTGAAAAGGCCAAAAAAGATATTGCTAGCTATTTCCACTTAGACAATATTTTAGACACTGACAACTTAGGGTTAAAAGCCACTGTTACCGAAAAAATCCAATTTACCGAAAAACAAATCGAAGCTGCAGTACAAAACAAAAAAGCAGCGATTATTTTTAAACCAGTGTTTACCGTCAACCAGTGTTTAATCCAACCAGATGCTGTAGTAGTACATGCTAATGGTTTGTGTGAGTTTGTTGTTATTAAAGCGACAACAAATACCAAGAGAAAATTTATCTTGGAAATTATTTATGACTTCTTGCTGTTTGAAAAATTGGGCAAATACAAACTGGTTAACTATTACTTCTGCATAGTTAATTATGAACTGAAAAATAAACACAATGTTTCCTTCTTTTTAAACACCGAAATTAAAACAGCCAAAAACAGTAGTACGAGTAAAACTAAAGAAGAGCAAGTGTTGTACGGGCATCTCCCTTTTAATGATCCAAAAAAAATTGCTTACATTCATAGCAAGAAGAGTGGTGGTGTCAATGGTTTTCTGCTCGTCAAACTAGTGGACAACATTATTAGAAGCGGTGTTACTAATTTGGAACAAATTATTAGTTTTGTTTTTCGTGAACTGAATGCACCCAGCATTCGTAGCTTAAAGCAAATTATTAGTGAAGTAGCTAAAGTACAACTTGATTTTTGGAACATTATTGATGATGTTAAACAACACCAAGAGTTACAAGACAACCAAATTACCTTCAATTACAGTGATGCCTTCAACTCTTTTTGGAATAACTATTTATTGCGCAATCTAATTAAATTAGTCTTTGCTTATAAATACAGTGAAATCTTTCGACTTTCAGGAAAATTAGCTAAATGA", "aa": "MLTKAFFLKNFDRSKELIPLSYADVFGAASQLLKKHHKQVDTEIDVQEDLIEDPVFEIDILELLNEAPELLFDSKNPRVKEAQIIIEKAKKDIASYFHLDNILDTDNLGLKATVTEKIQFTEKQIEAAVQNKKAAIIFKPVFTVNQCLIQPDAVVVHANGLCEFVVIKATTNTKRKFILEIIYDFLLFEKLGKYKLVNYYFCIVNYELKNKHNVSFFLNTEIKTAKNSSTSKTKEEQVLYGHLPFNDPKKIAYIHSKKSGGVNGFLLVKLVDNIIRSGVTNLEQIISFVFRELNAPSIRSLKQIISEVAKVQLDFWNIIDDVKQHQELQDNQITFNYSDAFNSFWNNYLLRNLIKLVFAYKYSEIFRLSGKLAK", "aa_hexdigest": "d7297195fa447fccef86a89f0f9c2dd4", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00031D7E73", "query_cov": 0.9973262032085561, "subject_cov": 0.8197802197802198, "identity": 0.627, "score": 470.0, "evalue": 7.74e-162, "valid": false, "uniref50_id": "UniRef50_UPI00031D7E73" }, "pscc": { "uniref50_id": "UniRef50_UPI00031D7E73", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00031D7E73" ] }, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 662826, "stop": 664253, "observations": { "mutation": [ 663948, 664092, 664140 ], "stop": [ 663948, 664092, 664140 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75234", "query_cov": 1.0, "subject_cov": 0.5632530120481928, "identity": 1.0, "score": 721.0, "evalue": 1.44e-257, "gene_start": 1, "gene_end": 374, "reference_start": 1, "reference_end": 374, "reference_sequence": "MLTKAFFLKNFDRSKELIPLSYADVFGAASQLLKKHHKQVDTEIDVQEDLIEDPVFEIDILELLNEAPELLFDSKNPRVKEAQIIIEKAKKDIASYFHLDNILDTDNLGLKATVTEKIQFTEKQIEAAVQNKKAAIIFKPVFTVNQCLIQPDAVVVHANGLCEFVVIKATTNTKRKFILEIIYDFLLFEKLGKYKLVNYYFCIVNYELKNKHNVSFFLNTEIKTAKNSSTSKTKEEQVLYGHLPFNDPKKIAYIHSKKSGGVNGFLLVKLVDNIIRSGVTNLEQIISFVFRELNAPSIRSLKQIISEVAKVQLDFWNIIDDVKQHQELQDNQITFNYSDAFNSFWNNYLLRNLIKLVFAYKYSEIFRLSGKLAKWDEVVEAYKENKSVKIDGFLYELNQGKMKKTKNPATSQFNKIHFFLRAWNDKKGIAVGNKFKSVWQKLKEKKVYFDFETISSAVRVIDKSLPFTQIVTQCSLIVDDNTESDKSKLVCQNLIFDPLTIGIEDFKTVVDALYQKQCDQYSFVVYNKSFEKNRLLEMATFINEAPYQQRVQAIIENLFDLADIFGLENDCLAFKQLDGFSSIKKVLPMIDQRFLDASRTVSYQSLKVQKGDVAQELTLARFLNCLDEQQWAQTALELKQYCENDVRAMIAIELFIKDFITNQL" }, "paralog": false, "identity": 0.9936842105263158, "score": 924.0, "evalue": 0.0, "description": "Internal stop codon at 663948, 664092, 664140. Nonsense mutation around 663948, 664092, 664140.", "psc": { "uniref90_id": "UniRef90_P75234", "product": "Uncharacterized protein MG366 homolog", "uniref50_id": "UniRef50_P47606", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75234", "UniRef:UniRef50_P47606" ] }, "pscc": { "uniref50_id": "UniRef50_P47606", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47606" ], "product": "Uncharacterized protein MG366" } }, "id": "APKECPOBEN_1179", "locus": "GCA002090215_01178" }, { "type": "cds", "sequence": "contig_1", "start": 663987, "stop": 664094, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAAAATTGATGGCTTTTTATACGAACTTAACCAAGGGAAAATGAAAAAAACTAAAAATCCCGCTACTTCCCAATTTAACAAAATCCACTTTTTTCTAAGAGCTTGA", "aa": "MKIDGFLYELNQGKMKKTKNPATSQFNKIHFFLRA", "aa_hexdigest": "88b619553b00c8d34a49e95a038c0045", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4145.848800000001, "isoelectric_point": 10.121810340881346 }, "id": "APKECPOBEN_1180", "locus": "GCA002090215_01179" }, { "type": "cds", "sequence": "contig_1", "start": 664236, "stop": 664718, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF11074.14" ], "nt": "GTGACCCAGTGTTCTCTTATTGTTGATGACAATACCGAGTCTGATAAAAGCAAGTTAGTTTGTCAAAACCTGATCTTTGATCCGCTAACTATTGGTATTGAGGACTTTAAAACAGTCGTTGATGCCCTTTACCAAAAGCAGTGTGACCAGTACAGTTTTGTCGTTTATAACAAATCGTTTGAAAAGAACAGGTTACTGGAAATGGCTACTTTTATCAATGAAGCTCCATACCAACAAAGAGTGCAAGCAATTATTGAAAATTTATTTGATTTAGCAGATATTTTTGGACTTGAAAATGATTGCTTAGCCTTTAAACAACTTGATGGTTTTTCATCAATTAAAAAGGTCTTGCCAATGATTGATCAACGCTTTTTAGATGCTAGTCGAACTGTTAGTTACCAATCATTAAAGGTACAAAAAGGTGATGTAGCACAAGAGCTTACTTTGGCACGCTTTTTAAATTGTTTAGACGAACAGCAATGA", "aa": "MTQCSLIVDDNTESDKSKLVCQNLIFDPLTIGIEDFKTVVDALYQKQCDQYSFVVYNKSFEKNRLLEMATFINEAPYQQRVQAIIENLFDLADIFGLENDCLAFKQLDGFSSIKKVLPMIDQRFLDASRTVSYQSLKVQKGDVAQELTLARFLNCLDEQQ", "aa_hexdigest": "0b066c4eafd93c818b983ce543e345ce", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF11074.14", "name": "DUF2779", "length": 112, "aa_cov": 0.69375, "hmm_cov": 0.9017857142857143, "evalue": 5.838535400246781e-15, "score": 51.15733337402344, "start": 2, "stop": 112 } ], "seq_stats": { "molecular_weight": 18385.77879999999, "isoelectric_point": 4.52236042022705 }, "id": "APKECPOBEN_1181", "locus": "GCA002090215_01180" }, { "type": "cds", "sequence": "contig_1", "start": 664861, "stop": 665481, "strand": "-", "frame": 1, "gene": "rnc", "product": "ribonuclease III", "db_xrefs": [ "COG:COG0571", "COG:K", "EC:3.1.26.3", "GO:0003723", "GO:0004525", "GO:0005737", "GO:0006364", "GO:0006396", "GO:0006397", "GO:0008033", "GO:0046872", "SO:0001217", "UniRef:UniRef50_P47607", "UniRef:UniRef90_P75233" ], "nt": "TTGGAATTTTTAGGTGATGCCTTAATTGATTTCATAGTAGCGAAAAAACTGTTTGAGCTTTATCCCAATTACAACGAAGGTATGTTGACCAGAACTAAGATTGAAATTGTTAAGGGCGAAAACCTCAACCGTATTGGGAAGGAATTGAACTTTGGCAATTTCATTAAGTTAGGTAAAGGAATGCCGTATACTGAAACACTTTTTGGTGATGTACTAGAGGCGTTAGTGGCAGCCATTTACGAAGACCTTGGCATAGAGAAAGCGAATCAATTTGTCGAAGAACATATTTTTAAAAAAACTTACTCAGAAATTCTCAAGTACAACTTCTTTTCACTGTTTCAAGAACAAAAGCTACCAGAACCTAGGGTACGGGTTAGTTTAACTAGCAATAACCTTGTGTTGAGTATTATTGAGCTTAACGGTGACATTATCTGGTCACAAGCTGTACCTAACAGTAAACACTATGATGACAAGAGCGTGTTAGAACACAATGCAATGTCAGCCTTTACCCAGTTTCTTAAAAGTGGTAAAGGCATTAACTTTTTTAGTGACATTAAAAATAAGTTAGACAGTCAAAAGCCTATGCGAGCTTTAACAGTTAGACCGAAAAAGATTAACTGA", "aa": "MEFLGDALIDFIVAKKLFELYPNYNEGMLTRTKIEIVKGENLNRIGKELNFGNFIKLGKGMPYTETLFGDVLEALVAAIYEDLGIEKANQFVEEHIFKKTYSEILKYNFFSLFQEQKLPEPRVRVSLTSNNLVLSIIELNGDIIWSQAVPNSKHYDDKSVLEHNAMSAFTQFLKSGKGINFFSDIKNKLDSQKPMRALTVRPKKIN", "aa_hexdigest": "737f0a18fe7873f149c09b795d6154a2", "start_type": "TTG", "rbs_motif": null, "genes": [ "rnc" ], "truncated": "both", "pseudogene": { "start": 664804, "stop": 665652, "observations": { "mutation": [ 664863, 665559 ], "stop": [ 664863, 665559 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75233", "query_cov": 1.0, "subject_cov": 0.7304964539007093, "identity": 0.995, "score": 407.0, "evalue": 1.52e-142, "gene_start": 1, "gene_end": 206, "reference_start": 58, "reference_end": 263, "reference_sequence": "MKNKKDKTQKPKVIDEKFVAFFKSLNIEPQNWQFYEDAFVHSSYVNENEDARASYDRLEFLGDALIDFIVAKKLFELYPNYNEGMLTRTKIEIVKGENLNRIGKELNFGNFIKLGKGMPYTETLFGDVLEALVAAIYEDLGIEKANQFVEEHIFKKTYSEILKYNFFSLFQEQKLPEPRVRVSLTSNNLVLSIIELNGDIIWSQAVPNSKHYDDKSVLEHNAMSAFTQFLKSGKGINFFSDIKNKLDSQKPMRALTVRPKKINWKARKPKLKALKNKVKADS" }, "paralog": false, "identity": 0.9929078014184397, "score": 552.0, "evalue": 4.03e-202, "description": "Internal stop codon at 664863, 665559. Nonsense mutation around 664863, 665559.", "psc": { "uniref90_id": "UniRef90_P75233", "gene": "rnc", "product": "ribonuclease III", "ec_ids": [ "3.1.26.3" ], "uniref50_id": "UniRef50_P47607", "cog_id": "COG0571", "cog_category": "K", "go_ids": [ "GO:0003723", "GO:0004525", "GO:0005737", "GO:0006364", "GO:0006396", "GO:0006397", "GO:0008033", "GO:0046872" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75233", "EC:3.1.26.3", "UniRef:UniRef50_P47607", "COG:COG0571", "COG:K", "GO:0003723", "GO:0004525", "GO:0005737", "GO:0006364", "GO:0006396", "GO:0006397", "GO:0008033", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_P47607", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47607" ], "product": "Ribonuclease 3" } }, "id": "APKECPOBEN_1182", "locus": "GCA002090215_01181" }, { "type": "cds", "sequence": "contig_1", "start": 665557, "stop": 665652, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAATAAGAAAGATAAAACTCAAAAACCTAAGGTCATTGATGAAAAGTTTGTCGCTTTTTTCAAGAGTTTAAACATTGAACCGCAAAACTGA", "aa": "MKNKKDKTQKPKVIDEKFVAFFKSLNIEPQN", "aa_hexdigest": "611bbaf44cf0d061be9744ba31977735", "start_type": "ATG", "rbs_motif": "AAAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3694.304400000001, "isoelectric_point": 9.778516197204588 }, "id": "APKECPOBEN_1183", "locus": "GCA002090215_01182" }, { "type": "cds", "sequence": "contig_1", "start": 665642, "stop": 666628, "strand": "-", "frame": 3, "gene": "plsX", "product": "phosphate acyltransferase PlsX", "db_xrefs": [ "COG:COG0416", "COG:I", "EC:2.3.1.274", "GO:0005737", "GO:0006633", "GO:0008610", "GO:0008654", "GO:0043811", "KEGG:K03621", "RefSeq:WP_010874903.1", "SO:0001217", "UniParc:UPI0000131C66", "UniRef:UniRef100_P75232", "UniRef:UniRef50_Q49427", "UniRef:UniRef90_P75232" ], "nt": "ATGGCTTTTAGACTAGCAGTTGATTGTTTAGGTTTTGAAAACCACCCGCGTGAAGCAATTGACGCAGTATTAGAATACTGGAGCTATCATCAAGAGTTGGAATTTATCTTAGTTGGTGATGAAAAGACCTTTGACGGACTTGATTATCTACCTAAAAACATTACCAAGCAATTAGCCAGTTCTTGCATAGATATGACCGACACACCACTAACCGCACGGCGCAAGGTTAACAACTCAATGCAGAAAGCGATTAACCTTGTGCGTGATGGTGCTGCTGATGTTGTGATTTCCGCTGGCTCCTCGGCCGTTTATGCCTCTTTAACATACGATGGCTTTGGCAAAATCCATAAAGATGTTAAAAGTGCTTTTATGTCTTATGTACCAACTGCCAATAACGACTGGTTTTACTTTTTAGATGTTGGCGCTAACAAGAACTTTACGGGTAAAGAGCTCTATTTTTTGGGCCTAATGGCGGATATTTTTGTCAAAAAGACCACGAACAAAATTAGTCCACGTATAGCATTGCTAAACATTGGTACCGAAATCCATAAAGGTTTTGATTACCACCAGGAAGGCTACCAATTGCTAAACGAAGATAAGCACCTTAACTTCACTGGTTTCATTGAACCACGCTTCTTACTTGACGGGGTGTGTGATATCTTAGTGGCTGATGGTTACAGCGGTAATCTTGTGTTGAAATCAATGGAAGGCACCTTTAAAACAATTGCGCGTCTTCTCAAGCAAGGTTATAAGCGTAACCCGTTGGCTGGGCTTTTTAGTTTGGGAATTTTGAAAAGAATTGCTAAGCGTTTTGATTACAAAAATAACGCAGGCGCCGTTGTTATCGGTTTAAATAAACTTGCTTTAAAAACCCATGGATCAGCTGATAAACAACAGTTTTTAAGCACCATTAGATTAGCACACACTAGCTTAAAGTCTGATCTAATTAACGCCATTAAAAGTTCCTTAGATAACTATGAAAAATAA", "aa": "MAFRLAVDCLGFENHPREAIDAVLEYWSYHQELEFILVGDEKTFDGLDYLPKNITKQLASSCIDMTDTPLTARRKVNNSMQKAINLVRDGAADVVISAGSSAVYASLTYDGFGKIHKDVKSAFMSYVPTANNDWFYFLDVGANKNFTGKELYFLGLMADIFVKKTTNKISPRIALLNIGTEIHKGFDYHQEGYQLLNEDKHLNFTGFIEPRFLLDGVCDILVADGYSGNLVLKSMEGTFKTIARLLKQGYKRNPLAGLFSLGILKRIAKRFDYKNNAGAVVIGLNKLALKTHGSADKQQFLSTIRLAHTSLKSDLINAIKSSLDNYEK", "aa_hexdigest": "ffd9716d0cd482bd4b5c37f0ff1829a5", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000131C66", "ncbi_nrp_id": "WP_010874903.1", "uniref100_id": "UniRef100_P75232", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000131C66", "RefSeq:WP_010874903.1", "UniRef:UniRef100_P75232" ] }, "ips": { "uniref100_id": "UniRef100_P75232", "uniref90_id": "UniRef90_P75232" }, "psc": { "uniref90_id": "UniRef90_P75232", "gene": "plsX", "product": "phosphate acyltransferase PlsX", "ec_ids": [ "2.3.1.274" ], "uniref50_id": "UniRef50_Q49427", "cog_id": "COG0416", "cog_category": "I", "kegg_orthology_id": "K03621", "go_ids": [ "GO:0005737", "GO:0006633", "GO:0008610", "GO:0008654", "GO:0043811" ] }, "pscc": { "uniref50_id": "UniRef50_Q49427", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49427" ], "product": "Phosphate acyltransferase" }, "genes": [ "plsX" ], "id": "APKECPOBEN_1184", "locus": "GCA002090215_01183" }, { "type": "cds", "sequence": "contig_1", "start": 666628, "stop": 667767, "strand": "-", "frame": 1, "gene": "fakA", "product": "putative MG369-like protein", "db_xrefs": [ "COG:COG1461", "COG:I", "GO:0004371", "GO:0006071", "SO:0001217", "UniRef:UniRef50_P75231", "UniRef:UniRef90_P75231" ], "nt": "ATGCTCCCAGTGTTAAAGGCCTCTGGTGTAGTAGATTCAGGTGCTTATGGGTTCGCTTGCTTTTTAGAGGGGATGTTGTCCTTTTATGGTGAAAAGGCTACTTTAAACGATGGTAAGCTCACCAGTGCTGAATTGAGCCAAATGACCATTAGCGGTGAAAAACACGTTACGGAAGAAGAGTTTGGTTACTGCACTGAATATGTCTTGAAACTGGGCATGTCAGTAAGTCAAGAAGTTGAAAAGCAGAAGTTTAACCAAAAGAAGTTTGAAAGTAAGGTGAGCAAAATTGCCACTTCGGTAGTAGTTGCTAGTGACAAAGATAACGGCTTTGTTAAAGTACACGCCCATACCGAAAAACCTAACCTGTTATTAGAACTTGGGCTTAACTATGGTGAGTTTGAACTCGTCAAAATTGAAAACATGAATTTACAGGTAGCGAAGCAAAAACCCGCTCCTGTGAAGCGCAACATTAAACCAGCCATAGTAGTGACTGTACCAACTGAAGCTTTTGCTGATCGGATTCGCGAAGACTATGACATTCAAGCTATCTTGTGTACCGATGACACCGGTGCACCTTCGGTCTTTTCGTTACTAGAAGCAGTCAAGCTAACTCATTCCAGTAACATTATTTTCTTGTTACATGACAAGAACTACTTTCTCTCTGCCAACGAAGCGTTAAAACAATTAAAGCACCAAAAGATTAGTGCTGATTGTGTGATGACTACCAACCCGATTGAATCACTAGCGGCGCTTACTGTTTTTAACAGTGACCTTAACATTCACACCAATGTCAAAACAATGCGCCGTTTTGTCAAAGGCTTTGCATCTGCAACCATTACTCAAGCTTCGAAAAAGTACAAGGAAAATCGGATTGAAGTTAATAAGGGTGACTTTATTGCTGTAGCTAATAACAGCATTTGTGTTAGTGAAAAAGAGCTTGTTCAATGCGTGTTCAACACCATTGACCACCTTTTGAAAAAGGTAAAAAAGCCGGAGTTTCTTTTAGCTTACTATGGTAAAGATATCACCGCTGAGGAAGCGGAAGCAATGAAGGAAAAAATAGAAAAGAAATACAAGCTTTTCTGTGAGTTTTCCCCAGGGGAACAAAAAGTTTTTTCTTATATACTAGGCATACAATAA", "aa": "MLPVLKASGVVDSGAYGFACFLEGMLSFYGEKATLNDGKLTSAELSQMTISGEKHVTEEEFGYCTEYVLKLGMSVSQEVEKQKFNQKKFESKVSKIATSVVVASDKDNGFVKVHAHTEKPNLLLELGLNYGEFELVKIENMNLQVAKQKPAPVKRNIKPAIVVTVPTEAFADRIREDYDIQAILCTDDTGAPSVFSLLEAVKLTHSSNIIFLLHDKNYFLSANEALKQLKHQKISADCVMTTNPIESLAALTVFNSDLNIHTNVKTMRRFVKGFASATITQASKKYKENRIEVNKGDFIAVANNSICVSEKELVQCVFNTIDHLLKKVKKPEFLLAYYGKDITAEEAEAMKEKIEKKYKLFCEFSPGEQKVFSYILGIQ", "aa_hexdigest": "1305cb2248b8843c5d3ec1e68f7fc9ab", "start_type": "ATG", "rbs_motif": null, "genes": [ "fakA" ], "truncated": "3-prime", "pseudogene": { "start": 666628, "stop": 668067, "observations": { "mutation": [ 667794 ], "stop": [ 667794 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75231", "query_cov": 1.0, "subject_cov": 0.6792114695340502, "identity": 1.0, "score": 732.0, "evalue": 1.09e-263, "gene_start": 1, "gene_end": 379, "reference_start": 180, "reference_end": 558, "reference_sequence": "MSTVNLSNFIDMLRLGCKNIANNFEYINQLNVFPVPDGDTGTNMKVTLSEAFKKLESEISHIKSFSDLGKSFTRDLLLFSRGNSGVIFSQIMKGFFSDMISTKTATETELGIEDFATAFIKAEEVAYKNVSKPVEGTMLTVIRLISTDFKNQKNRAKTVQKLFEQVIKTAWQTVKKTPQMLPVLKASGVVDSGAYGFACFLEGMLSFYGEKATLNDGKLTSAELSQMTISGEKHVTEEEFGYCTEYVLKLGMSVSQEVEKQKFNQKKFESKVSKIATSVVVASDKDNGFVKVHAHTEKPNLLLELGLNYGEFELVKIENMNLQVAKQKPAPVKRNIKPAIVVTVPTEAFADRIREDYDIQAILCTDDTGAPSVFSLLEAVKLTHSSNIIFLLHDKNYFLSANEALKQLKHQKISADCVMTTNPIESLAALTVFNSDLNIHTNVKTMRRFVKGFASATITQASKKYKENRIEVNKGDFIAVANNSICVSEKELVQCVFNTIDHLLKKVKKPEFLLAYYGKDITAEEAEAMKEKIEKKYKLFCEFSPGEQKVFSYILGIQ" }, "paralog": false, "identity": 0.9979123173277662, "score": 929.0, "evalue": 0.0, "description": "Internal stop codon at 667794. Nonsense mutation around 667794.", "psc": { "uniref90_id": "UniRef90_P75231", "gene": "FakA", "product": "Uncharacterized protein MG369 homolog", "uniref50_id": "UniRef50_P75231", "cog_id": "COG1461", "cog_category": "I", "go_ids": [ "GO:0004371", "GO:0006071" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75231", "UniRef:UniRef50_P75231", "COG:COG1461", "COG:I", "GO:0004371", "GO:0006071" ] }, "pscc": { "uniref50_id": "UniRef50_P75231", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75231" ], "product": "Uncharacterized protein MG369 homolog" } }, "id": "APKECPOBEN_1185", "locus": "GCA002090215_01184" }, { "type": "cds", "sequence": "contig_1", "start": 667792, "stop": 668304, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCTACAGTAAATTTAAGCAACTTTATTGACATGTTGCGTCTTGGTTGTAAGAATATAGCTAATAATTTCGAATACATTAATCAACTCAATGTTTTCCCGGTGCCAGATGGTGATACGGGGACAAACATGAAGGTCACGTTGAGTGAAGCGTTCAAAAAACTAGAGAGTGAAATTAGCCACATTAAGAGCTTTAGTGATTTGGGCAAAAGCTTTACCCGTGACCTTTTACTTTTCTCACGCGGTAACTCTGGTGTTATTTTTTCCCAAATTATGAAGGGCTTTTTTAGTGACATGATTAGCACTAAAACAGCCACCGAAACCGAACTTGGCATAGAGGATTTTGCTACAGCTTTTATAAAGGCCGAAGAGGTGGCATACAAGAATGTCTCCAAGCCCGTAGAGGGTACGATGCTCACGGTTATTCGCTTAATTAGCACAGACTTTAAAAACCAAAAGAACCGTGCCAAAACAGTGCAAAAACTGTTTGAACAAGTAATTAAAACAGCCTGA", "aa": "MSTVNLSNFIDMLRLGCKNIANNFEYINQLNVFPVPDGDTGTNMKVTLSEAFKKLESEISHIKSFSDLGKSFTRDLLLFSRGNSGVIFSQIMKGFFSDMISTKTATETELGIEDFATAFIKAEEVAYKNVSKPVEGTMLTVIRLISTDFKNQKNRAKTVQKLFEQVIKTA", "aa_hexdigest": "52f3e1424588af7cb74f9eee66caf169", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19027.6914, "isoelectric_point": 8.62466106414795 }, "id": "APKECPOBEN_1186", "locus": "GCA002090215_01185" }, { "type": "cds", "sequence": "contig_1", "start": 668392, "stop": 668727, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTAACAGTTAAGGCCAAACCATTTCCTAACGCTAAACCGATTACCACCATTTTTGAAAACAGTAAGTACTTAACTAAGTTAGATCTTTCGTTGCTTACTATACGCTTAGTAAGTGGTAAAACCCACCAAATTCGTGCTTGCTTAAACCTCTTGAATACCCAGTTAGTTGGTGATAGAAAATACCAATTACTGCAATTTAAAAACCGTAACCGCGATTTTAAACACCAAGCCTTACATGCCACAAATCTAAGTTTTGCCCAATTAGATAAGCAGAAATTTCCCTTACTGGCTAATTACTCGCAACGGCAGTTTAAAAGCCAACTAGTACCGTGA", "aa": "MVTVKAKPFPNAKPITTIFENSKYLTKLDLSLLTIRLVSGKTHQIRACLNLLNTQLVGDRKYQLLQFKNRNRDFKHQALHATNLSFAQLDKQKFPLLANYSQRQFKSQLVP", "aa_hexdigest": "c96dc94f449ef1bc5722ce1f013a8783", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12855.907599999984, "isoelectric_point": 10.4221040725708 }, "id": "APKECPOBEN_1187", "locus": "GCA002090215_01186" }, { "type": "cds", "sequence": "contig_1", "start": 668749, "stop": 669339, "strand": "-", "frame": 1, "gene": "rluA", "product": "putative RNA pseudouridine synthase MG370-like protein", "db_xrefs": [ "COG:COG0564", "COG:J", "EC:5.4.99.-", "GO:0000455", "GO:0003723", "GO:0120159", "SO:0001217", "UniRef:UniRef50_P47610", "UniRef:UniRef90_P75230" ], "nt": "ATGTCAACAGCTAAGTTTGTAGTGCCTAAAGCTGTTGAAAACGTTAGGATTGAAAAGTTTTGTTTAAAGTTGTTGCCCAACATTAAGCTAAGTCAGTTCTTTAAATTGTTGCGCTTAGGTAAGGTCTTGCTCAATAACACGAAGGCTAAATTAGGTCAGCGTGTTAGCAGCGGTGATACAATCGTTTTTCAGTTTGCAATTGAACCTTACTTACACAACCACAGCGAAGTAGTGGATTTAACCCAAGTCCAAGATGATTTGCAGGTAATCTTTGAGGATGAACAGCTCATTGTGGTGGACAAACCAGCGGGTGTAGTGTGTCAACCCGATGCTAAACACGAACTGTTTAATTTAGCTAACAGCTTACTGAAACACTGTGGTTATAATCAATACTTTAAAAACAGTCTTAGCTTCATACCACGGTTTGCGCACCGAATTGATCGTAATACCTGTGGTTTGGTAATAGGTGCTAAAACCAACCAGGCATTACAGGAGTTAGAGGCGGTTTTTCGCCATAACTTGTTAATGAAACAATACCAAGGATTAGTGTTTGGCCCATTTAATTTTAAGGGCACACAAAAAGCTTATTGA", "aa": "MSTAKFVVPKAVENVRIEKFCLKLLPNIKLSQFFKLLRLGKVLLNNTKAKLGQRVSSGDTIVFQFAIEPYLHNHSEVVDLTQVQDDLQVIFEDEQLIVVDKPAGVVCQPDAKHELFNLANSLLKHCGYNQYFKNSLSFIPRFAHRIDRNTCGLVIGAKTNQALQELEAVFRHNLLMKQYQGLVFGPFNFKGTQKAY", "aa_hexdigest": "6e8b5124e74d0f1360d9faa9fdf7b300", "start_type": "ATG", "rbs_motif": null, "genes": [ "rluA" ], "truncated": "5-prime", "pseudogene": { "start": 668446, "stop": 669339, "observations": { "mutation": [ 668751 ], "stop": [ 668751 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75230", "query_cov": 1.0, "subject_cov": 0.6012269938650306, "identity": 1.0, "score": 390.0, "evalue": 1.67e-135, "gene_start": 1, "gene_end": 196, "reference_start": 1, "reference_end": 196, "reference_sequence": "MSTAKFVVPKAVENVRIEKFCLKLLPNIKLSQFFKLLRLGKVLLNNTKAKLGQRVSSGDTIVFQFAIEPYLHNHSEVVDLTQVQDDLQVIFEDEQLIVVDKPAGVVCQPDAKHELFNLANSLLKHCGYNQYFKNSLSFIPRFAHRIDRNTCGLVIGAKTNQALQELEAVFRHNLLMKQYQGLVFGPFNFKGTQKAYWAKDAYQALVTVKAKPFPNAKPITTIFENSKYLTKLDLSLLTIRLVSGKTHQIRACLNLLNTQLVGDRKYQLLQFKNRNRDFKHQALHATNLSFAQLDKQKFPLLANYSQRQFKSQLVPWFASLIKGVSI" }, "paralog": false, "identity": 0.9966329966329966, "score": 588.0, "evalue": 1.37e-215, "description": "Internal stop codon at 668751. Nonsense mutation around 668751.", "psc": { "uniref90_id": "UniRef90_P75230", "gene": "RluA", "product": "Uncharacterized RNA pseudouridine synthase MG370 homolog", "ec_ids": [ "5.4.99.-" ], "uniref50_id": "UniRef50_P47610", "cog_id": "COG0564", "cog_category": "J", "go_ids": [ "GO:0003723", "GO:0120159", "GO:0000455" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75230", "EC:5.4.99.-", "UniRef:UniRef50_P47610", "COG:COG0564", "COG:J", "GO:0003723", "GO:0120159", "GO:0000455" ] }, "pscc": { "uniref50_id": "UniRef50_P47610", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47610" ], "product": "Uncharacterized RNA pseudouridine synthase MG370" } }, "id": "APKECPOBEN_1188", "locus": "GCA002090215_01187" }, { "type": "cds", "sequence": "contig_1", "start": 669326, "stop": 669448, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTGGTGGTGGTGGGCACAAGTATGCTGCTGCCTTTGTGTTGGATGACAAGCGTCAGTTTATGAAGTTAGTGGAAATTATGGATGACTTTTTAGCCAAACAAAAGCATGTCAACAGCTAA", "aa": "MFGGGGHKYAAAFVLDDKRQFMKLVEIMDDFLAKQKHVNS", "aa_hexdigest": "6ad50d8c0e8924b3ec1958914c3de93b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4544.239600000001, "isoelectric_point": 8.140052223205569 }, "id": "APKECPOBEN_1189", "locus": "GCA002090215_01188" }, { "type": "cds", "sequence": "contig_1", "start": 669497, "stop": 670303, "strand": "-", "frame": 3, "gene": "nrnA", "product": "putative MG371-like protein", "db_xrefs": [ "COG:COG0618", "COG:FT", "EC:3.1.13.3", "EC:3.1.3.7", "GO:0003676", "KEGG:K06881", "SO:0001217", "UniRef:UniRef50_P75229", "UniRef:UniRef90_P75229" ], "nt": "ATGATTAACATCGATCCCCATTTTATTCATAACCTAACTAATAAACTCAAGACATTCGATAACTTTTCGTTATATGTGCATGTAAACCCGGACTTTGATGCCTTTGGCGCTGCTTTTGCCTTTAAGGCCTTTTTAGCGGTTTACTTCCCCCATAAAAAGGCTTACGTGATGGGATCACACAACATTAAGGCTGACGGGAAAGATTTGTTCCCCTTTGAAGCGGCACCTATTGATGATGCGTTTGTAAAAAACTCATTGGCAATTATCTTTGACACCTCCAACCAAGAACGAGTATTAACCCAAAAACATAAATTAGCTAAAGAAACCGTGCGGATTGATCACCATCCTAAAACCGAGAGTTTTGCTGATCTGGAATGGATTGACCCGGCTTTTTCAGCAGCAGCAGAAATGGTCGGTTACTTAATCTTGCAAATGGGTTATGAACTTAATGCTGAGATGGCTGCTTACATTTATGCGGGTATTATTACCGATACACAGCGCTTTTCCAGTTCCGCTACCACCCCACAAACCTTTGCTTTAACTGCTAAGTTACTAGAAACTGGATTTAACCGCAATAAGGTTCATGATGCAGTTTATCTAAAACCACTTTTAGAACACAAGTACTTTAGCTATGTCTTAAACAAAGCCAAGATCACCCCGAATGGTTTGGCTTACGCACTTCTTAAAAAAGGGACTTATAAGCAGTTTGGGGTGGTCTCACCACTACCGATGGTGCACGCTTTAAACAACATCAAAGGAGTCAAAATTTGGACGACTTGTTACTTTAACGAAGACATTAAGAAGTGA", "aa": "MINIDPHFIHNLTNKLKTFDNFSLYVHVNPDFDAFGAAFAFKAFLAVYFPHKKAYVMGSHNIKADGKDLFPFEAAPIDDAFVKNSLAIIFDTSNQERVLTQKHKLAKETVRIDHHPKTESFADLEWIDPAFSAAAEMVGYLILQMGYELNAEMAAYIYAGIITDTQRFSSSATTPQTFALTAKLLETGFNRNKVHDAVYLKPLLEHKYFSYVLNKAKITPNGLAYALLKKGTYKQFGVVSPLPMVHALNNIKGVKIWTTCYFNEDIKK", "aa_hexdigest": "7ecaa9433da3a58a934864d1378b3540", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75229", "query_cov": 1.0, "subject_cov": 0.8246153846153846, "identity": 1.0, "score": 538.0, "evalue": 9.88e-193, "valid": true, "gene": "NrnA", "product": "Uncharacterized protein MG371 homolog", "ec_ids": [ "3.1.13.3", "3.1.3.7" ], "uniref50_id": "UniRef50_P75229", "cog_id": "COG0618", "cog_category": "FT", "kegg_orthology_id": "K06881", "go_ids": [ "GO:0003676" ] }, "pscc": { "uniref50_id": "UniRef50_P75229", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75229" ], "product": "Uncharacterized protein MG371 homolog" }, "genes": [ "nrnA" ], "id": "APKECPOBEN_1190", "locus": "GCA002090215_01189" }, { "type": "cds", "sequence": "contig_1", "start": 670296, "stop": 670433, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATACCTGTACTGTGTTTGCTCCCAAAAAACCAACTACTAAGGCTAAGTTAGCGATCGTTGAAAAGCTTGAGAGTGAACTTTTGTTTGTCCGTGAACTAATTGAAGCTGGTGTCAAGAAGCTGCAAAATGATTAA", "aa": "MDTCTVFAPKKPTTKAKLAIVEKLESELLFVRELIEAGVKKLQND", "aa_hexdigest": "b23bbd64bd2dfe4cacb12c9dbca1bfea", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5046.941199999999, "isoelectric_point": 7.871863746643066 }, "id": "APKECPOBEN_1191", "locus": "GCA002090215_01190" }, { "type": "cds", "sequence": "contig_1", "start": 670476, "stop": 671459, "strand": "-", "frame": 2, "gene": "thiI", "product": "putative tRNA sulfurtransferase", "db_xrefs": [ "COG:COG0301", "COG:HJ", "EC:2.8.1.4", "GO:0000049", "GO:0002937", "GO:0004810", "GO:0005524", "GO:0005829", "GO:0009228", "GO:0009229", "GO:0052837", "GO:0140741", "KEGG:K03151", "SO:0001217", "UniRef:UniRef50_P47612", "UniRef:UniRef90_P75228" ], "nt": "ATGGGATTGCATAATGAACCAAATACTATTCTAATCTGCCGTTATGGCGAACTTGTTTTAAAGGGCAAGAACCGCTTGCAGTTTGTCAAACAACTCAAAAAGAACGTTAAACAAGCCTTTAAGAAACTAAGCATTACTAACCCAGTTGACTACCAGTTTGACATGTTGGTAGTGGGTGAAGTAATTTCCACCCAAAGATCGTTATTAAAAAATCTCTTTACCAGATTACCGGGTTTGAGTGTTTGCTTGTTTGCCTTACAAATCCCTCATGATGAAGCGCAATTGTTAGCACTGCTACAGCAAGTGGTACAATCCCACCCATCGTTCAAAATTGAGGTAAGACGGCGTGATAAATTATTTGCTTGCAATTCCAGTGCCTTCAAAAAGTACCTAGCTTTACAGTTATGGGAAAAATACCAATTGAAGGGTAAGTTAGTTGACCCTGCCATTACGGTGCATGTCGAAGTAACTAAGGAACATTTCCTAATAATTAGTGAAAGCTTTAATGGTATTGGTGGTCTACCGGTCTTTACTAGTGGTACTGCTTTGGCGTTACTGTCTGGTGGCATAGACAGTCCAGTAGCCGCTTCCCTAGTTTTACAACGAGGTTTTAATGTTGACTTTATTACTTTTATTAATGAGCCCGGACATAACGCTGCCACAATAGGCAAAATTCAACGGTTAGCTAACTTAGTTTCCCTTAACCAAACGCTTTGTACTGGGAGACTGTTTGTCTTTGACTTTACCGATTTACAGAAAGAGTTGAGTCATATTAGTCTGGAAGGATACCGGATTGTTTTAATGCGCCGTTGCTTTTACAAGATTGCTAGTTTGTTTAAATATGATTGTTTAATCACCGGGGAAGCATTGGGACAAGTAGCTTCACAAACAATTGATAATTTGAAGGTAATCCAAGCTGTTGTGCCCAATACCTTTGTGATTCGCCCGTTAATAGGCTTAAGTAAAGATAAAATAATTGAATGA", "aa": "MGLHNEPNTILICRYGELVLKGKNRLQFVKQLKKNVKQAFKKLSITNPVDYQFDMLVVGEVISTQRSLLKNLFTRLPGLSVCLFALQIPHDEAQLLALLQQVVQSHPSFKIEVRRRDKLFACNSSAFKKYLALQLWEKYQLKGKLVDPAITVHVEVTKEHFLIISESFNGIGGLPVFTSGTALALLSGGIDSPVAASLVLQRGFNVDFITFINEPGHNAATIGKIQRLANLVSLNQTLCTGRLFVFDFTDLQKELSHISLEGYRIVLMRRCFYKIASLFKYDCLITGEALGQVASQTIDNLKVIQAVVPNTFVIRPLIGLSKDKIIE", "aa_hexdigest": "88c17433a7a858e83239292eb5fef1b1", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75228", "query_cov": 1.0, "subject_cov": 0.8449612403100775, "identity": 1.0, "score": 635.0, "evalue": 6e-229, "valid": true, "gene": "thiI", "product": "Probable tRNA sulfurtransferase", "ec_ids": [ "2.8.1.4" ], "uniref50_id": "UniRef50_P47612", "cog_id": "COG0301", "cog_category": "HJ", "kegg_orthology_id": "K03151", "go_ids": [ "GO:0005829", "GO:0005524", "GO:0004810", "GO:0000049", "GO:0140741", "GO:0009228", "GO:0009229", "GO:0052837", "GO:0002937" ] }, "pscc": { "uniref50_id": "UniRef50_P47612", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47612" ], "product": "Probable tRNA sulfurtransferase" }, "genes": [ "thiI" ], "id": "APKECPOBEN_1192", "locus": "GCA002090215_01191" }, { "type": "cds", "sequence": "contig_1", "start": 671470, "stop": 672117, "strand": "-", "frame": 1, "gene": null, "product": "MPN551 family DNA-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75227", "UniRef:UniRef90_P75227" ], "nt": "ATGGTCAAGATTTATGAGGATGAATTTGATGAAACACTCTCACGCGCGGGGCAAGTTATTGAACCCAAAATCCGTGATTATGTGATAGCTAAAACTGGTTTTAATTTCCATTCGTATGATCCCAAGGAGGTTAAGTGGGATCTCTTTCCTGAAAATCCAGTTTTTGGAGGAATTCCTGATGGGGAACCGGTCGATGTTTATGGAAAATTGGCTTACGATACCAATGCGCCGATGTTAGAAATTAAAACCACTTCCTGTGATTCTTTGGTCTATAAAAAGATTAACGGTAACCTCAAAATTGTGTTTGATGAAAACGGCATGCCGATTGTCAAAAAGATTAACGGAAAGAAAGATAGTTGGTTTGACAGTAACGGCAAAATCGTGATTAGCCCGGCTTACTATTGCCAAATTGGGTTGTATTTGTACTTGCGCAATATTACCAAAGGAATGTTTGCGATTGCTTTTTTAGAACCACAAGACTATGTGCATCCCGAATGGTTTGAAGCAAAGCAACGCGACATCCGTTTGGTACCAGTACAAATTGATCGCAAGGCCTTTGAAGTTTTAACTAATAAAGCACAGCTGTGGTACAACAGTTTTATTCGTACTGGTAAAAGTCCACAATTAACCTCACAAGACTGGGAGTGA", "aa": "MVKIYEDEFDETLSRAGQVIEPKIRDYVIAKTGFNFHSYDPKEVKWDLFPENPVFGGIPDGEPVDVYGKLAYDTNAPMLEIKTTSCDSLVYKKINGNLKIVFDENGMPIVKKINGKKDSWFDSNGKIVISPAYYCQIGLYLYLRNITKGMFAIAFLEPQDYVHPEWFEAKQRDIRLVPVQIDRKAFEVLTNKAQLWYNSFIRTGKSPQLTSQDWE", "aa_hexdigest": "c4594b244de3c8e7953141338c4ad21f", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 671446, "stop": 672291, "observations": { "mutation": [ 671472, 672126 ], "stop": [ 671472, 672126 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75227", "query_cov": 1.0, "subject_cov": 0.7651245551601423, "identity": 1.0, "score": 410.0, "evalue": 8.99e-144, "gene_start": 1, "gene_end": 215, "reference_start": 59, "reference_end": 273, "reference_sequence": "MGFTKQYKIDFDIIDNQIVLSNNYFQKHKGSFRKITGTRFGKVIGISEYETSLKTWANMVKIYEDEFDETLSRAGQVIEPKIRDYVIAKTGFNFHSYDPKEVKWDLFPENPVFGGIPDGEPVDVYGKLAYDTNAPMLEIKTTSCDSLVYKKINGNLKIVFDENGMPIVKKINGKKDSWFDSNGKIVISPAYYCQIGLYLYLRNITKGMFAIAFLEPQDYVHPEWFEAKQRDIRLVPVQIDRKAFEVLTNKAQLWYNSFIRTGKSPQLTSQDWEWLRENGIA" }, "paralog": false, "identity": 0.9928825622775801, "score": 534.0, "evalue": 1.11e-194, "description": "Internal stop codon at 671472, 672126. Nonsense mutation around 671472, 672126.", "psc": { "uniref90_id": "UniRef90_P75227", "product": "MPN551 family DNA-binding protein", "uniref50_id": "UniRef50_P75227", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75227", "UniRef:UniRef50_P75227" ] }, "pscc": { "uniref50_id": "UniRef50_P75227", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75227" ], "product": "Uncharacterized protein MG373 homolog" } }, "id": "APKECPOBEN_1193", "locus": "GCA002090215_01192" }, { "type": "cds", "sequence": "contig_1", "start": 672124, "stop": 672291, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGATTTACCAAACAGTACAAAATCGATTTTGATATTATTGACAACCAAATAGTTTTGTCAAACAACTATTTTCAAAAACATAAAGGTAGTTTTCGCAAGATTACCGGTACCCGATTTGGTAAAGTAATAGGTATTTCTGAGTATGAAACTTCACTTAAAACTTGA", "aa": "MGFTKQYKIDFDIIDNQIVLSNNYFQKHKGSFRKITGTRFGKVIGISEYETSLKT", "aa_hexdigest": "cc6b4530b501482e28ce0c7bb25e98ba", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6407.267899999999, "isoelectric_point": 9.630819129943848 }, "id": "APKECPOBEN_1194", "locus": "GCA002090215_01193" }, { "type": "cds", "sequence": "contig_1", "start": 672298, "stop": 672960, "strand": "-", "frame": 1, "gene": null, "product": "MPN552 family protein", "db_xrefs": [ "GO:0005524", "SO:0001217", "UniRef:UniRef50_P75226", "UniRef:UniRef90_P75226" ], "nt": "GTGTATCTGTTAGATTTACTTTCACTCAACAACGTTGATGTAATTGCGGAATCAGGTGCCATTACCTGGTTTTGCAAAACCAATCAGTATGACTATCAAGCCTTTTTAGACCTCGCTAGTATTAATGTCATTATCCACCACGCTGTGATTACCAATTCCGGGATCTTTACCATGGGTAAAAGCCGCGTTGATGATACCGCTAATTTATCAGCTAACTATTTCATTTCACTACAAAAGTACAAGGACTTTAAGTCGCTGTGGTTAACGGACTTTGAACAAACCTTAAAGTACGAAAACTTTTTAAAACAACTCGGTAAATTGGAACTCAGTTCTATCTACGTCTTTAGTCCCCAATACCACATGGACTTAGCTTTTATTGACCAAATTGCTAGTGGTCAACCACGCTTTACCCACAGCAATTTCTATCCCAATAACCTGTTGTTTACAAGTAATAAAGTAACTAAATTTAATGCACTGGAAAAATACACTAAGACCCAAGGCTTAATGCTTAAAGATGTGCACTACATTAACTTAGATGAAACTTTGGTGCAAAACAGTGAGCAGTTAGCTAGTGCTGTTTTCATCAAGAAACAGAACAATGAAGGTTTAACAGTGCAGGACATTCCTGATGTGTTACAAAAGCTTTGTGCACAACTCTTGTAA", "aa": "MYLLDLLSLNNVDVIAESGAITWFCKTNQYDYQAFLDLASINVIIHHAVITNSGIFTMGKSRVDDTANLSANYFISLQKYKDFKSLWLTDFEQTLKYENFLKQLGKLELSSIYVFSPQYHMDLAFIDQIASGQPRFTHSNFYPNNLLFTSNKVTKFNALEKYTKTQGLMLKDVHYINLDETLVQNSEQLASAVFIKKQNNEGLTVQDIPDVLQKLCAQLL", "aa_hexdigest": "ff2b4e6e901bfbcab640c7cd4c669444", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75226", "query_cov": 1.0, "subject_cov": 0.8178438661710037, "identity": 0.995, "score": 438.0, "evalue": 7.68e-155, "valid": true, "product": "MPN552 family protein", "uniref50_id": "UniRef50_P75226", "go_ids": [ "GO:0005524" ] }, "pscc": { "uniref50_id": "UniRef50_P75226", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75226" ], "product": "Uncharacterized protein MG374 homolog" }, "genes": [], "id": "APKECPOBEN_1195", "locus": "GCA002090215_01194" }, { "type": "cds", "sequence": "contig_1", "start": 673136, "stop": 673396, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03129.25" ], "nt": "ATGGCACCAGTACAAGTAGTGGTGATTCCGGTTAACATCGATAAACACGAAAAGGCTGCCAAAAAACTGTACCAAAAATTGTTAAAGGAAAACATTAGGGTCAGCCTAGATGAGAGCGAAGATCGTTTGGGCAAAAAAGTTCGCACAGCCATTGTTAACAAGATTCCACTGCAAATTATTGTCGGCGATAAGGAGATGCAGGATCTTACTCGAATAACTTGTCGTGGCTTTAAAGGTGAGAAGGTACAGAAACTGTATTGA", "aa": "MAPVQVVVIPVNIDKHEKAAKKLYQKLLKENIRVSLDESEDRLGKKVRTAIVNKIPLQIIVGDKEMQDLTRITCRGFKGEKVQKLY", "aa_hexdigest": "f0d2dd0b06db86d9cf87c7e0ae22a61e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03129.25", "name": "HGTP_anticodon", "length": 80, "aa_cov": 0.9069767441860465, "hmm_cov": 0.9875, "evalue": 1.8059412508444893e-21, "score": 71.61233520507812, "start": 5, "stop": 82 } ], "seq_stats": { "molecular_weight": 9857.613199999996, "isoelectric_point": 9.716755485534666 }, "id": "APKECPOBEN_1196", "locus": "GCA002090215_01195" }, { "type": "cds", "sequence": "contig_1", "start": 673397, "stop": 673675, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00587.30" ], "nt": "GTGTTTAAGGAGTTAAATGTGCCCTATAAAATTGATGTGGGTGCAGCTGCTTTTTATGGACCTAAAATTGACTTTCAGTTTAAAACGGCACTCAATAAGATGATTACCATTGCCACTATTCAACTGGATTTTTTACTGCCAGAACGCTTTGAATTGCGTTACTTTGATGAGCAGAGCAATGCTCAAACTCCCGTAATTATTCACGTTGGCATAGTCGGTACGTACGAGCGTTTTATTGCTGCTTTATTGGAAAAAACTCATGGCAATCTACCGTTGTGA", "aa": "MFKELNVPYKIDVGAAAFYGPKIDFQFKTALNKMITIATIQLDFLLPERFELRYFDEQSNAQTPVIIHVGIVGTYERFIAALLEKTHGNLPL", "aa_hexdigest": "fda05000102537b83a3dfef45eb140e3", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00587.30", "name": "tRNA-synt_2b", "length": 72, "aa_cov": 0.7608695652173914, "hmm_cov": 0.9722222222222222, "evalue": 3.7080509510146256e-15, "score": 51.888248443603516, "start": 16, "stop": 85 } ], "seq_stats": { "molecular_weight": 10505.126599999989, "isoelectric_point": 6.054114341735839 }, "id": "APKECPOBEN_1197", "locus": "GCA002090215_01196" }, { "type": "cds", "sequence": "contig_1", "start": 673703, "stop": 674221, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00587.30" ], "nt": "TTGGAAAAGGGAGCAGTTTTAAAAAAGATTATTGGTGATTTTGTCCACTACCAACAGTTAATGTTTGGTTTTAAAACGGTATCTTCACCAGTATTAGCTAACTTAGAGCTGTACAAGATTAGTGGTCACTATGTGCACTATAGTGAGGACATGTTCCCCTCTGTCAAATTAGAAAACCAAATGATGATGCTCCGGCCAATGACCTGTCCGCACCACTGTTTGATTTTCAAGCACAAACGCCGCTCTTACAAAGAGTTACCACAACGCTTCTGTGAGGATTCCATCTTGCACCGCTTTGAAGCATCGGGTGGGCTTACTGGTTTGGAACGGGTAAGGTGCATGACACTGCTCGACAACCACATCTTTTGTCGTACTGACCAAATTAAAGCTGAGATTAAAAATGCCTTTAAGTTAATCCAAACGGTCAACAACAAGTTTGGTTTTAGCTTTGACCGAATTGACTTGGCCTTACACGATCCTAATAACAAGGCCAAATTCATTGACAACGATCAGTTATGA", "aa": "MEKGAVLKKIIGDFVHYQQLMFGFKTVSSPVLANLELYKISGHYVHYSEDMFPSVKLENQMMMLRPMTCPHHCLIFKHKRRSYKELPQRFCEDSILHRFEASGGLTGLERVRCMTLLDNHIFCRTDQIKAEIKNAFKLIQTVNNKFGFSFDRIDLALHDPNNKAKFIDNDQL", "aa_hexdigest": "2b75d7c9c35898e5159a9b5f73e2e4ec", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00587.30", "name": "tRNA-synt_2b", "length": 104, "aa_cov": 0.5930232558139535, "hmm_cov": 0.9807692307692307, "evalue": 2.6335866576792055e-20, "score": 68.66114807128906, "start": 58, "stop": 159 } ], "seq_stats": { "molecular_weight": 20113.2449, "isoelectric_point": 8.953191947937015 }, "id": "APKECPOBEN_1198", "locus": "GCA002090215_01197" }, { "type": "cds", "sequence": "contig_1", "start": 674222, "stop": 674686, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGAAAAGAGCTTTTCCATTAAAGAGTTTGAACAGCTCGAACAGGACTTAAATGCTTATTTTAATCAGTTAAATAATGTTGTTCAGACAAAAATTGATAAGACAAAATCACTCAAATTTTTTCAAGCTGATCCGTTTACAACCACGCTTATTGAAAGTATAGAAAGCAACAGCCTTGTAGTTACCACCGTAAATGAACAAACATTTTGGGTGCATGATTTAGTGTTGCCATTACCCAAACAGGGCTTTGTGAAACTGCTCAATGTTAGTGCCAATTATTTTTTAGGTGATCCCACAAAACCACAACTACAAAGGCTTGTGGGAATTTTTGCAGCCTCTAAAGAACAGCTTAAAAGTCTTATTGCGGCTAATGAACAAAAATACCAAAATGATCACCGTGCTATTGGTAAGCGTTTAGAGATCTTTACCTTTGATCCCTTAGTCGGTGCAGGCTTTCCGATTTGA", "aa": "MEKSFSIKEFEQLEQDLNAYFNQLNNVVQTKIDKTKSLKFFQADPFTTTLIESIESNSLVVTTVNEQTFWVHDLVLPLPKQGFVKLLNVSANYFLGDPTKPQLQRLVGIFAASKEQLKSLIAANEQKYQNDHRAIGKRLEIFTFDPLVGAGFPI", "aa_hexdigest": "5b7ca69845cf2cf926b94364f10abb75", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17524.850199999983, "isoelectric_point": 6.116296195983886 }, "id": "APKECPOBEN_1199", "locus": "GCA002090215_01198" }, { "type": "cds", "sequence": "contig_1", "start": 674866, "stop": 675021, "strand": "-", "frame": 1, "gene": null, "product": "putative MG376-like protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75224", "UniRef:UniRef90_P75224" ], "nt": "ATGCGCTTTTTTGGCGAGCGTTTATTTACTGACTATTACGTCATTTATGCCAATGGTCAACTGGCTTACGAATTGGAGAAACACACCAAGAAGTACAAGACCATTAGTATTGAAGGCATTTTGCGCACTTATTTAGAGCGTAAGAGTGAAATTTGA", "aa": "MRFFGERLFTDYYVIYANGQLAYELEKHTKKYKTISIEGILRTYLERKSEI", "aa_hexdigest": "46c870f8326c63aabc3fc298666ec418", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 674794, "stop": 675108, "observations": { "mutation": [ 674868, 675063 ], "stop": [ 674868, 675063 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75224", "query_cov": 1.0, "subject_cov": 0.4636363636363636, "identity": 1.0, "score": 102.0, "evalue": 5.16e-27, "gene_start": 1, "gene_end": 51, "reference_start": 36, "reference_end": 86, "reference_sequence": "GGGGGGMLNRVFLEGEIESSCWSVKKTGFLVTIKQMRFFGERLFTDYYVIYANGQLAYELEKHTKKYKTISIEGILRTYLERKSEIWKTTIEIVKIFNPKNEIVIDYKEI" }, "paralog": false, "identity": 0.9807692307692307, "score": 200.0, "evalue": 4.32e-68, "description": "Internal stop codon at 674868, 675063. Nonsense mutation around 674868, 675063.", "psc": { "uniref90_id": "UniRef90_P75224", "product": "Uncharacterized protein MG376 homolog", "uniref50_id": "UniRef50_P75224", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75224", "UniRef:UniRef50_P75224" ] }, "pscc": { "uniref50_id": "UniRef50_P75224", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75224" ], "product": "Uncharacterized protein MG376 homolog" } }, "id": "APKECPOBEN_1200", "locus": "GCA002090215_01199" }, { "type": "cds", "sequence": "contig_1", "start": 675110, "stop": 675691, "strand": "-", "frame": 3, "gene": null, "product": "MPN555 family protein chaperone", "db_xrefs": [ "GO:0006457", "GO:0015031", "RefSeq:WP_010874912.1", "SO:0001217", "UniParc:UPI00001396F1", "UniRef:UniRef100_P75223", "UniRef:UniRef50_P75223", "UniRef:UniRef90_P75223" ], "nt": "ATGGCTACAAATCTTAAATCAACCGCTAAACTAGTTAAACCAATTCAGTACGATGAGGTAATTGAAGTAGAACGCATCTTTGCTGACCCTGCTTTTATTGAACAACACCGCCAACGGATCTTAGCTTCCTTTAAGGACGCTAAGGAAAGTGCGCTTTATCATGAACTAACGCACATTGTCATTAAGGACAACCTCTTTTCCTGTGCCATGAATGCTATTGTGGGTTACTTTGAATTCAACATCGATGAAGCTGAATTGAAGAATGTCATGGAAGGTCTAAAGCGTGATGTGATTCAGGGCGCTGAGGACAACACCGTGCAAGCAATTGCTGAAAAAATTATTAAGAAGGCTTTAGTGTTTAACCACCTCCAAAAAGAATGGAAAGTGGAAATCACTGATGAAGTGGTGAAGAATGTAATTAGTCTTTATTACGAAAAAACCAATCAAAGTGTGCGCGAATACTTGGATGATAAACAAAAGTTTGAGGGTGTGCGTACAGCCTTGCTAGAAGAGCGCATGGTGTTAGAAACCATCAACCACTTTAAATTTCACTTTAACTTAACTGGACAATTACCAAACTAA", "aa": "MATNLKSTAKLVKPIQYDEVIEVERIFADPAFIEQHRQRILASFKDAKESALYHELTHIVIKDNLFSCAMNAIVGYFEFNIDEAELKNVMEGLKRDVIQGAEDNTVQAIAEKIIKKALVFNHLQKEWKVEITDEVVKNVISLYYEKTNQSVREYLDDKQKFEGVRTALLEERMVLETINHFKFHFNLTGQLPN", "aa_hexdigest": "cc9fcabe515c72c0b2d1c8146e89e4ef", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001396F1", "ncbi_nrp_id": "WP_010874912.1", "uniref100_id": "UniRef100_P75223", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001396F1", "RefSeq:WP_010874912.1", "UniRef:UniRef100_P75223" ] }, "ips": { "uniref100_id": "UniRef100_P75223", "uniref90_id": "UniRef90_P75223" }, "psc": { "uniref90_id": "UniRef90_P75223", "product": "MPN555 family protein chaperone", "uniref50_id": "UniRef50_P75223", "go_ids": [ "GO:0006457", "GO:0015031" ] }, "pscc": { "uniref50_id": "UniRef50_P75223", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75223" ], "product": "Uncharacterized protein MG377 homolog" }, "genes": [], "id": "APKECPOBEN_1201", "locus": "GCA002090215_01200" }, { "type": "cds", "sequence": "contig_1", "start": 675734, "stop": 676522, "strand": "-", "frame": 3, "gene": "argS", "product": "arginine--tRNA ligase", "db_xrefs": [ "COG:COG0018", "COG:J", "EC:6.1.1.19", "GO:0004814", "GO:0005524", "GO:0005737", "GO:0006420", "KEGG:K01887", "SO:0001217", "UniRef:UniRef50_P47618", "UniRef:UniRef90_P75222" ], "nt": "TTGAAGACTACGCTCTATGGTGATGATAAGGACCGGGTGTTAGTGCGTCAAGACAATCGGCCTTCTTACTTTGGTACCGATGTCGCTTACCACTTAGACAAGGCTGCGCGGGGCTTTGACCTCTTATACGACATTTGGGGCAGTGATCATGAAGGGCACATTAAACGGATGCACTGTGTTTATGAGGGACTAAAGATTCACCAAAAATGCCAGCTCAAAATTACTGCCTTACAGTTGGTAATGCTGTACAAGAATAAGGAAATAGTCAGATTGTCCAAACGTGCTGGCAACGTTATTACCATTAAGCAAATGTTGCAAATGCTCAGTGAGGATGCGGCGCGTTGGTTTATGTTGTCGCAAACCAACAACTCGATTATCAAGATTGATTTGGACACTGCTAACCTGCAAAACTCTTCCAATCCGGTTTATTACGTGCAATATGCGTATGCCAGAATGTGCAGTGTATTAAAGGTAGTGGATCAAGCAGCACTAGCTCAAGTAAATGACTGCAGCTTATTAACGCATGAAAAGGAAATTGCTTTACTAGACCAGTTAGTGTACTTTAAGAGCTTACTGGAAAAAGTTCAGGTTAGCCATGAACTGCACCTCTTAACTAATTACTTATACGAGACAGCTACCTTGTTTCATTCTTGGTATAAGGCTTGCAAAATTAACGATCCAGCGCAGTACAATTTAACCCAGCAACGCTTACTTTTACTGCAATCATTGCACCATGTTTTTGGTCAGCTATTGCAAATCTTAAACATTACTGCACCACAACAAATGTAA", "aa": "MKTTLYGDDKDRVLVRQDNRPSYFGTDVAYHLDKAARGFDLLYDIWGSDHEGHIKRMHCVYEGLKIHQKCQLKITALQLVMLYKNKEIVRLSKRAGNVITIKQMLQMLSEDAARWFMLSQTNNSIIKIDLDTANLQNSSNPVYYVQYAYARMCSVLKVVDQAALAQVNDCSLLTHEKEIALLDQLVYFKSLLEKVQVSHELHLLTNYLYETATLFHSWYKACKINDPAQYNLTQQRLLLLQSLHHVFGQLLQILNITAPQQM", "aa_hexdigest": "d3a64c3b9aaab12963d011da547d34d9", "start_type": "TTG", "rbs_motif": null, "genes": [ "argS" ], "truncated": "3-prime", "pseudogene": { "start": 675734, "stop": 676822, "observations": { "mutation": [ 676525 ], "stop": [ 676525 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75222", "query_cov": 1.0, "subject_cov": 0.48789571694599626, "identity": 0.996, "score": 520.0, "evalue": 4e-182, "gene_start": 1, "gene_end": 262, "reference_start": 276, "reference_end": 537, "reference_sequence": "MLFINTDLQECLNALNLEFDEHKELVKLVKNNSFSGFASTVVFHLKGVNQKETAQQIAAWLLKHKKAHYRRVFVANNNFINFEISPQKYLDFLKTKPTFAPKPTKVLIEWVSANPTGELHLGHVRNAFFGHVLNNLMVFLGFQTVREYWVNDYGQQARVFGFSVYQALHLQQNIKVTPHPDGYEGELVDSIAKTITGIPLDKLSFEEFLQQPFLDQLLADCTAKVLEVIKQDLATIHIHFDSWKFESQVVKETDYKKLLTQFKDEAHYEKDGAIWLKTTLYGDDKDRVLVRQDNRPSYFGTDVAYHLDKAARGFDLLYDIWGSDHEGHIKRMHCVYEGLKIHQKCQLKITALQLVMLYKNKEIVRLSKRAGNVITIKQMLQMLSEDAARWFMLSQTNNSIIKIDLDTANLQNSSNPVYYVQYAYARMCSVLKVVDQAALAQVNDCSLLTHEKEIALLDQLVYFKSLLEKVQVSHELHLLTNYLYETATLFHSWYKACKINDPAQYNLTQQRLLLLQSLHHVFGQLLQILNITAPQQM" }, "paralog": false, "identity": 0.9972375690607734, "score": 725.0, "evalue": 1.95e-265, "description": "Internal stop codon at 676525. Nonsense mutation around 676525.", "psc": { "uniref90_id": "UniRef90_P75222", "gene": "argS", "product": "arginine--tRNA ligase", "ec_ids": [ "6.1.1.19" ], "uniref50_id": "UniRef50_P47618", "cog_id": "COG0018", "cog_category": "J", "kegg_orthology_id": "K01887", "go_ids": [ "GO:0004814", "GO:0005524", "GO:0005737", "GO:0006420" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75222", "EC:6.1.1.19", "UniRef:UniRef50_P47618", "COG:COG0018", "COG:J", "KEGG:K01887", "GO:0004814", "GO:0005524", "GO:0005737", "GO:0006420" ] }, "pscc": { "uniref50_id": "UniRef50_P47618", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47618" ], "product": "Arginine--tRNA ligase" } }, "id": "APKECPOBEN_1202", "locus": "GCA002090215_01201" }, { "type": "cds", "sequence": "contig_1", "start": 676523, "stop": 677347, "strand": "-", "frame": 3, "gene": "argS", "product": "arginine--tRNA ligase", "db_xrefs": [ "COG:COG0018", "COG:J", "EC:6.1.1.19", "GO:0004814", "GO:0005524", "GO:0005737", "GO:0006420", "KEGG:K01887", "SO:0001217", "UniRef:UniRef50_P47618", "UniRef:UniRef90_P75222" ], "nt": "ATGCTTTTCATTAACACAGATCTTCAAGAATGTCTTAACGCTTTGAATCTAGAATTTGATGAGCACAAAGAGTTAGTCAAACTTGTTAAAAACAACAGCTTTAGTGGTTTTGCTTCCACCGTTGTGTTCCACTTAAAGGGTGTAAATCAAAAGGAAACCGCCCAACAGATTGCTGCTTGGTTGTTAAAACACAAAAAGGCGCATTACCGCCGTGTGTTTGTCGCTAATAACAACTTCATTAACTTTGAAATTAGCCCTCAAAAATACCTCGATTTTTTAAAAACCAAACCTACTTTTGCCCCTAAACCAACTAAGGTTCTAATTGAATGGGTGTCGGCTAACCCCACGGGTGAGTTGCACTTAGGTCATGTCCGCAATGCCTTCTTTGGTCATGTACTCAATAACCTGATGGTTTTTTTAGGCTTTCAAACAGTTCGGGAATACTGGGTCAATGACTATGGTCAACAAGCACGGGTGTTTGGTTTTAGTGTGTACCAAGCACTCCACTTACAACAAAACATTAAGGTCACTCCTCACCCTGATGGTTATGAGGGGGAGCTAGTGGACAGTATTGCCAAAACTATTACCGGTATTCCTTTAGATAAACTCAGCTTTGAAGAGTTTTTACAACAACCCTTTCTGGACCAGTTACTAGCTGACTGTACTGCCAAAGTCTTGGAAGTAATTAAGCAAGACTTAGCTACAATCCACATTCACTTTGACAGCTGGAAGTTTGAAAGTCAAGTGGTTAAGGAAACAGATTACAAAAAACTGTTAACCCAGTTTAAGGACGAAGCGCACTATGAAAAGGATGGTGCCATTTGA", "aa": "MLFINTDLQECLNALNLEFDEHKELVKLVKNNSFSGFASTVVFHLKGVNQKETAQQIAAWLLKHKKAHYRRVFVANNNFINFEISPQKYLDFLKTKPTFAPKPTKVLIEWVSANPTGELHLGHVRNAFFGHVLNNLMVFLGFQTVREYWVNDYGQQARVFGFSVYQALHLQQNIKVTPHPDGYEGELVDSIAKTITGIPLDKLSFEEFLQQPFLDQLLADCTAKVLEVIKQDLATIHIHFDSWKFESQVVKETDYKKLLTQFKDEAHYEKDGAI", "aa_hexdigest": "c94a838e5467e08923c311b171eed8a2", "start_type": "ATG", "rbs_motif": null, "genes": [ "argS" ], "truncated": "5-prime", "pseudogene": { "start": 676220, "stop": 677347, "observations": { "mutation": [ 676525 ], "stop": [ 676525 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75222", "query_cov": 1.0, "subject_cov": 0.5102420856610801, "identity": 1.0, "score": 555.0, "evalue": 9.72e-196, "gene_start": 1, "gene_end": 274, "reference_start": 1, "reference_end": 274, "reference_sequence": "MLFINTDLQECLNALNLEFDEHKELVKLVKNNSFSGFASTVVFHLKGVNQKETAQQIAAWLLKHKKAHYRRVFVANNNFINFEISPQKYLDFLKTKPTFAPKPTKVLIEWVSANPTGELHLGHVRNAFFGHVLNNLMVFLGFQTVREYWVNDYGQQARVFGFSVYQALHLQQNIKVTPHPDGYEGELVDSIAKTITGIPLDKLSFEEFLQQPFLDQLLADCTAKVLEVIKQDLATIHIHFDSWKFESQVVKETDYKKLLTQFKDEAHYEKDGAIWLKTTLYGDDKDRVLVRQDNRPSYFGTDVAYHLDKAARGFDLLYDIWGSDHEGHIKRMHCVYEGLKIHQKCQLKITALQLVMLYKNKEIVRLSKRAGNVITIKQMLQMLSEDAARWFMLSQTNNSIIKIDLDTANLQNSSNPVYYVQYAYARMCSVLKVVDQAALAQVNDCSLLTHEKEIALLDQLVYFKSLLEKVQVSHELHLLTNYLYETATLFHSWYKACKINDPAQYNLTQQRLLLLQSLHHVFGQLLQILNITAPQQM" }, "paralog": false, "identity": 0.9973333333333333, "score": 763.0, "evalue": 5.45e-280, "description": "Internal stop codon at 676525. Nonsense mutation around 676525.", "psc": { "uniref90_id": "UniRef90_P75222", "gene": "argS", "product": "arginine--tRNA ligase", "ec_ids": [ "6.1.1.19" ], "uniref50_id": "UniRef50_P47618", "cog_id": "COG0018", "cog_category": "J", "kegg_orthology_id": "K01887", "go_ids": [ "GO:0004814", "GO:0005524", "GO:0005737", "GO:0006420" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75222", "EC:6.1.1.19", "UniRef:UniRef50_P47618", "COG:COG0018", "COG:J", "KEGG:K01887", "GO:0004814", "GO:0005524", "GO:0005737", "GO:0006420" ] }, "pscc": { "uniref50_id": "UniRef50_P47618", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47618" ], "product": "Arginine--tRNA ligase" } }, "id": "APKECPOBEN_1203", "locus": "GCA002090215_01202" }, { "type": "cds", "sequence": "contig_1", "start": 677375, "stop": 679213, "strand": "+", "frame": 2, "gene": "mnmG", "product": "tRNA uridine-5-carboxymethylaminomethyl(34) synthesis enzyme MnmG", "db_xrefs": [ "COG:COG0445", "COG:J", "GO:0002098", "GO:0005829", "GO:0008033", "GO:0030488", "GO:0050660", "KEGG:K03495", "RefSeq:WP_010874914.1", "SO:0001217", "UniParc:UPI000012B445", "UniRef:UniRef100_P75221", "UniRef:UniRef50_P75221", "UniRef:UniRef90_P75221" ], "nt": "ATGTCTTTTACACTAACAGTTATTGGTGGCGGTCATGCCGGATTAGAGGCCGCATTTATTGCTAGTAAACTAGGGCTAAAAGTTAACCTCTTAGTACTAGATCCCAACCATGTGGGGTCGTGTCCCTGTAACCCGGCCATAGGAGGTCCTGCTAAAGGTATAGTAACCCGGGAAATCGATGTTTTAGGAGGGATGCAGGGTAAGGCCGCTGATGCCACTGCTTTACAATACAAGCTGCTCAACAGCTCGAAAGGTCCAGCAGTGCAAGCAATCCGCGCTCAAATAGACAAAATTGCTTATCAGAAGTGGTTTCGCCAGCAAATTGACCAAACGCCTAACATTGAGTTAATTGCTGGGGAAGCCGTTGATATCCTTGAAAGTAACGGTAAAGTTAAAGGTGTCGTGTTAGCTGATGGTAGTGAACTGGCATCAGATGCTGTTATTGTGACTACGGGAACTTATCTAAAGGCCAAAACTTACTGTGGTAGCTTAAGCAAAGAAGAAGGTCCTGACCGCGCTAAGCGCAGTGAATACCTTTCCACTAACTTAATTAAGCGGGGTTTTAAAACGTTACGACTAAAAACTGGGACACCACCACGGATCCTACGCGAATCACTCGACTTTAGCCAAATGGCTGTGGAAGCTAACACTACCCCTCATTTAGCCTTTAGCTTTACTACTAAGAATTATTTACCACTAGAACAGCAAGTGATCTGTCACTTAATCCATACCAATCCCCAGATCCATCAGTTAATTCTGGCCAACTTAAAACAGTCCGCTGTCTTTAACGGTAGTATTAAAGCAAACGGACCACTTTACTGTCCGAGTATTGAAGACAAGGTATTTCGCTTTCAAGACAAAGAACGGCACCAAATCTTTGTCGAACCTGAATCACTTAGTTTAGAAACGGTTTACCTAGCAGGGTTTTCCACTTCCTTTCCCCCAGAGGTGCAAGAGCACATTGTCCGTTTACTCCCTGGGTTTAAAAATGCCCGGTTTCAAAAGTATGGCTATGCCATTGAATATGATGCCTTTAGTTCGATACAACTCAAATCAACACTAGAAACTAAGTTAATTCAAAATCTGTATTTTGCCGGACAGATTAATGGTACCAGTGGGTATGAGGAAGCAGCTGGCCAGGGCTTAATCGCTGGGATTAACGCCGCTTTAAAGCTACAGCGTAAACCCGAATTTGTGTTACAGCGCAATGAGGCATACCTTGGTGTCATGATTAACGACCTGGTTACTAAGGAAATTAGTGATCCCTACCGCTTGTTAACTTCGCGCGCTGAACACCGTTTGTGGTTGCGCAATGATAACTTACAAGAACGTCTAATTGAAAAGAGCCGAGCCTTAGGTTTAGTGGAAGCAGATGTGTATGCCAATTACTTGGAACAACAACAAAAGAAAAAACAATTAATTGATTATTTGCAAACCACTACCGTTGGGCAAATTGCTGCCTTGAAATTAAACTTTAAAAACACGGCCCAAACGCTGTTTGACTTTACCAAGCGTGCTGAAATTAAGCTGGTTGATTTAGTACAACTACTCCCCAAGCGTTTTGACTTGGATGTACAAAGTTTAAACCAGATTGACATTGACATTAAGTACGCTGGTTACATTAAGAAGAGTGAAAAGTACTTCAAGAGTTTAAACAACCTCAGTAGTGTCAAAATTCCCTTAAAGTTAAATTACCACAAAGTACCTAACTTAGCGAGTGAAGCAATTGTTAAACTCTCCAAAATTAGACCAACTGATCTCAGTGTAGCTAGTCAAGTAGCGGGCATTAACTTTAACGATATTTTAGCGATTAAACATTTTTTAGATAACCATGAATAA", "aa": "MSFTLTVIGGGHAGLEAAFIASKLGLKVNLLVLDPNHVGSCPCNPAIGGPAKGIVTREIDVLGGMQGKAADATALQYKLLNSSKGPAVQAIRAQIDKIAYQKWFRQQIDQTPNIELIAGEAVDILESNGKVKGVVLADGSELASDAVIVTTGTYLKAKTYCGSLSKEEGPDRAKRSEYLSTNLIKRGFKTLRLKTGTPPRILRESLDFSQMAVEANTTPHLAFSFTTKNYLPLEQQVICHLIHTNPQIHQLILANLKQSAVFNGSIKANGPLYCPSIEDKVFRFQDKERHQIFVEPESLSLETVYLAGFSTSFPPEVQEHIVRLLPGFKNARFQKYGYAIEYDAFSSIQLKSTLETKLIQNLYFAGQINGTSGYEEAAGQGLIAGINAALKLQRKPEFVLQRNEAYLGVMINDLVTKEISDPYRLLTSRAEHRLWLRNDNLQERLIEKSRALGLVEADVYANYLEQQQKKKQLIDYLQTTTVGQIAALKLNFKNTAQTLFDFTKRAEIKLVDLVQLLPKRFDLDVQSLNQIDIDIKYAGYIKKSEKYFKSLNNLSSVKIPLKLNYHKVPNLASEAIVKLSKIRPTDLSVASQVAGINFNDILAIKHFLDNHE", "aa_hexdigest": "60eda10628024d38f59690539339f17b", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000012B445", "ncbi_nrp_id": "WP_010874914.1", "uniref100_id": "UniRef100_P75221", "db_xrefs": [ "SO:0001217", "UniParc:UPI000012B445", "RefSeq:WP_010874914.1", "UniRef:UniRef100_P75221" ] }, "ips": { "uniref100_id": "UniRef100_P75221", "uniref90_id": "UniRef90_P75221" }, "psc": { "uniref90_id": "UniRef90_P75221", "gene": "mnmG", "product": "tRNA uridine-5-carboxymethylaminomethyl(34) synthesis enzyme MnmG", "uniref50_id": "UniRef50_P75221", "cog_id": "COG0445", "cog_category": "J", "kegg_orthology_id": "K03495", "go_ids": [ "GO:0002098", "GO:0005829", "GO:0008033", "GO:0030488", "GO:0050660" ] }, "pscc": { "uniref50_id": "UniRef50_P75221", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75221" ], "product": "tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG" }, "genes": [ "mnmG" ], "id": "APKECPOBEN_1204", "locus": "GCA002090215_01203" }, { "type": "cds", "sequence": "contig_1", "start": 679206, "stop": 679781, "strand": "+", "frame": 3, "gene": "rsmG", "product": "16S rRNA (guanine(527)-N(7))-methyltransferase RsmG", "db_xrefs": [ "COG:COG0357", "COG:J", "EC:2.1.1.-", "EC:2.1.1.170", "GO:0005829", "GO:0008168", "GO:0031167", "GO:0046118", "GO:0070043", "KEGG:K03501", "RefSeq:WP_010874915.1", "SO:0001217", "UniParc:UPI000012B477", "UniRef:UniRef100_P75220", "UniRef:UniRef50_P75220", "UniRef:UniRef90_P75220" ], "nt": "ATGAATAACAGTAAATTGAAACAGTATGTTCAACTAGTACAAACAGCCAACCAAAACTTTAACCTCACCGGTCTTAAAACTGAAGGCGAAATTTACGAACACTTAGTCCAAGAAATTATCGAGCTCTTTAATGAGTATGACAGTTACTTTGACCATAAAAAGGTTGCAGATTTGGGTAGTGGAAATGGTTGTCCTGGTGTCATCTTAAAGCTGTTGTTTCCCCAAATCAAAACTTTGGATTTAATTGATAGCAAACACAAAAAGGTTAACTTTTTAAAAGAGGTGATTCAAACACTGGAACTAAACAATACCCAAGCACTTTGTGCCAGAATTGAAAACCACACTGAACAATACGACACTTTATGCTCCCGCGGACTTGGCAGCATTATTGAGGTTAATGCCTTTGCTTTAAAACTGTTAAAGCCCAACGGCATTATCTTCCACATTAAACAAAGCTTAGATCAATACCTCGAGTTTGAGGATTCGGAGCAAAAAGACCAGTTTAAACCACTGTTCTTTAAGTTTTTCCATGGCAAGCGCCAGCAAATCCTGATTGCCATGAAGAAAAATGTTTAA", "aa": "MNNSKLKQYVQLVQTANQNFNLTGLKTEGEIYEHLVQEIIELFNEYDSYFDHKKVADLGSGNGCPGVILKLLFPQIKTLDLIDSKHKKVNFLKEVIQTLELNNTQALCARIENHTEQYDTLCSRGLGSIIEVNAFALKLLKPNGIIFHIKQSLDQYLEFEDSEQKDQFKPLFFKFFHGKRQQILIAMKKNV", "aa_hexdigest": "be8301c57ed36447e490b8d2609dca06", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000012B477", "ncbi_nrp_id": "WP_010874915.1", "uniref100_id": "UniRef100_P75220", "db_xrefs": [ "SO:0001217", "UniParc:UPI000012B477", "RefSeq:WP_010874915.1", "UniRef:UniRef100_P75220" ] }, "ips": { "uniref100_id": "UniRef100_P75220", "uniref90_id": "UniRef90_P75220" }, "psc": { "uniref90_id": "UniRef90_P75220", "gene": "rsmG", "product": "16S rRNA (guanine(527)-N(7))-methyltransferase RsmG", "ec_ids": [ "2.1.1.-", "2.1.1.170" ], "uniref50_id": "UniRef50_P75220", "cog_id": "COG0357", "cog_category": "J", "kegg_orthology_id": "K03501", "go_ids": [ "GO:0005829", "GO:0008168", "GO:0031167", "GO:0046118", "GO:0070043" ] }, "pscc": { "uniref50_id": "UniRef50_P75220", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75220" ], "product": "Ribosomal RNA small subunit methyltransferase G" }, "genes": [ "rsmG" ], "id": "APKECPOBEN_1205", "locus": "GCA002090215_01204" }, { "type": "cds", "sequence": "contig_1", "start": 679831, "stop": 679983, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAATTTGCCAAACAGTTTTATGTAACTAGTAGTGCTGGTGCAATTCTGTTTGACCTTAAAAAGAGTCAGGGCTTAGACCAACTGCAAGCAATTGAAAAAACAAGGAAGGTTATTATTACAGAACAGTTTGCCAACCAAACTGGTAAATGA", "aa": "MEFAKQFYVTSSAGAILFDLKKSQGLDQLQAIEKTRKVIITEQFANQTGK", "aa_hexdigest": "71181d2970941635dc4be202fd1bff47", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5622.407499999999, "isoelectric_point": 9.22402362823486 }, "id": "APKECPOBEN_1206", "locus": "GCA002090215_01205" }, { "type": "cds", "sequence": "contig_1", "start": 679987, "stop": 680208, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTGACAAGGAAAACACTAAACGGATTAACAGCTTAGCGCAAGAGCACATTACTCCCTTAATAAAACGGATTTTACGCTTAGCGGACTTTAAAAATGTCTTGATTAATGTGCAGCACCACAAGAAGCTGCAAAAATGTCTGTTATGGGAAATTAACGGTTTAATCTGCTTAGTTGAAAGTTTACAGTTCATGGAAAATCCCACTGCAATCATGGAATGA", "aa": "MFDKENTKRINSLAQEHITPLIKRILRLADFKNVLINVQHHKKLQKCLLWEINGLICLVESLQFMENPTAIME", "aa_hexdigest": "907136105473540eec5b93e8c71ce90e", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8614.161799999998, "isoelectric_point": 8.712273597717285 }, "id": "APKECPOBEN_1207", "locus": "GCA002090215_01206" }, { "type": "tRNA", "sequence": "contig_1", "start": 680443, "stop": 680518, "strand": "-", "gene": "trnR", "product": "tRNA-Arg(tct)", "amino_acid": "Arg", "anti_codon": "tct", "score": 74.8, "nt": "GCGTCGGTAGCTCAGCGGATAGAGTACACGCCTTCTAAGTGTGTTGTCGTGGGTTCGAGTCCCACCCGATGCGCCA", "db_xrefs": [ "SO:0001036" ], "anti_codon_pos": [ 680483, 680485 ], "id": "APKECPOBEN_18", "locus": "GCA002090215_01207" }, { "type": "cds", "sequence": "contig_1", "start": 680520, "stop": 681836, "strand": "-", "frame": 2, "gene": "arcA", "product": "Arginine deiminase-like protein", "db_xrefs": [ "COG:COG2235", "COG:E", "GO:0016990", "GO:0019546", "RefSeq:WP_010874917.1", "SO:0001217", "UniParc:UPI0000125DB1", "UniRef:UniRef100_P75218", "UniRef:UniRef50_P75218", "UniRef:UniRef90_P75218" ], "nt": "ATGTCAAAAAAACAGCTTGTCAAAACCGATGGGCATAACCAATTAGATCAACCAAACACTAAAGCACTACAGTTAAAAAAGAAGCAGTTTAACAGTGGTGTACGCGTTACTAGTGAAATTAGTTTTTTAAGGGAAGTAATTGCCCATCATCCCGGTATCGAAACCGAACGAGTGATTGATAACCAGACCTTTGGCAGTGCAATGTACTTAGAACGCGCTCAAAAGGAACACCAGTTATTTATTAAGATTTTGCGCCAACATGGTACAAAGGTGCACTATTTACAAGATTTACTGTTAGAAGCTTTAAGTGCGGCTGACCCTAATGTGCGCCAGGACTTCATTAAGAACTTTTTATTGGAATCAGGCATTAAAAGTGTGAGTACCTTCGAAGCTTGCTTAAACTTCTTTCGCAGTTTAGATTCGCTTGTAGATGTGATTAAGGTCATGTTTGGTGGCATTAAGGTATCAGATGTACCACCGATTACCCCGCAACGGTTTGCCGACATTCATGTAAGTAATTCACCGTTTTTAATTAAACCGCTATCGTTTTCCCTTTATCCACATAAGTTCTTCAATACCCTAGGAACGGGTGTCGCTTTATTTGTCACTAATGATTCTGAGTTAAAGCGCCATTCGTTAGTGTATGAATACATCATGCGCTTTCACCCCCGTTTTGATGGAGTTAAGCTTTATACTAACCGTGACTTTAAGAACTGTTTGATTAACAGTAGTGACATTATTCAAATTAGCAATGAAATCTTGCTAATTGGTATTAGTCATGACACCGATGTGTTGGGAATTGAAAGCTTGGCACGTAATTTATTAAGCGATCACACTAATCCAATTAAGCAGATTATTGCAATTAATATCCACAAGTTTGGTGCTAAGACCAATCTCAATAAACTTATAGCGATGGTGGATGTCGACAAGTTCATTATTGCGCGCAAAGTGTTACAAGCTACCGAAATCTTTGAACTCACAGCCACCGCCCAGAGGGATGTGGATGGCTTAGCACAAATTAAATTCAAACCGCTAAAGTTTAATTTTGGTGAGATAATTGAAGCGATTATTGATAAACAACCCCGCTTTGTTATTATTGGTGGTGGTGATGAAGTGGCTGAGCGTAAAGAACTCTTGGACTGTGGTATGGGCGTATTAAACTTAAGCCCTGGAGAAATTGTGGTCTTTGATCGCAACCACTACACCAATAATCTTCTCAATGAACTGGGATTAATCATCCATAAGATCCCCGCTTCCGAACTTTCACGGGGTCCTAGTGGCCCCTTGGAAATGGTTTGCTCTCTCTGGAGAGAATAA", "aa": "MSKKQLVKTDGHNQLDQPNTKALQLKKKQFNSGVRVTSEISFLREVIAHHPGIETERVIDNQTFGSAMYLERAQKEHQLFIKILRQHGTKVHYLQDLLLEALSAADPNVRQDFIKNFLLESGIKSVSTFEACLNFFRSLDSLVDVIKVMFGGIKVSDVPPITPQRFADIHVSNSPFLIKPLSFSLYPHKFFNTLGTGVALFVTNDSELKRHSLVYEYIMRFHPRFDGVKLYTNRDFKNCLINSSDIIQISNEILLIGISHDTDVLGIESLARNLLSDHTNPIKQIIAINIHKFGAKTNLNKLIAMVDVDKFIIARKVLQATEIFELTATAQRDVDGLAQIKFKPLKFNFGEIIEAIIDKQPRFVIIGGGDEVAERKELLDCGMGVLNLSPGEIVVFDRNHYTNNLLNELGLIIHKIPASELSRGPSGPLEMVCSLWRE", "aa_hexdigest": "779244268347b9d84ffb03fd1fa41faa", "start_type": "ATG", "rbs_motif": "AAAA", "ups": { "uniparc_id": "UPI0000125DB1", "ncbi_nrp_id": "WP_010874917.1", "uniref100_id": "UniRef100_P75218", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000125DB1", "RefSeq:WP_010874917.1", "UniRef:UniRef100_P75218" ] }, "ips": { "uniref100_id": "UniRef100_P75218", "uniref90_id": "UniRef90_P75218" }, "psc": { "uniref90_id": "UniRef90_P75218", "gene": "ArcA", "product": "Arginine deiminase-like protein", "uniref50_id": "UniRef50_P75218", "cog_id": "COG2235", "cog_category": "E", "go_ids": [ "GO:0016990", "GO:0019546" ] }, "pscc": { "uniref50_id": "UniRef50_P75218", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75218" ], "product": "Arginine deiminase-like protein" }, "genes": [ "arcA" ], "id": "APKECPOBEN_1208", "locus": "GCA002090215_01208" }, { "type": "cds", "sequence": "contig_1", "start": 681890, "stop": 682096, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00485.24" ], "nt": "ATGGATAGTAAAAAAGGAATTTTAGTGGCAATTGGTGGCGGGAGTTGTTCTGGTAAAACTACGATCGCTGACATGATTTACCAACTTTTGCGTAAAAAGCTTAAAGTTGCTATTCTGCCCCAGGACAACTATTACAAACCATACAAGAACAAATCAATGGCGCAACGCAAAGCGATTAACTTCGATCACCCCGATGCCTTTGATTGA", "aa": "MDSKKGILVAIGGGSCSGKTTIADMIYQLLRKKLKVAILPQDNYYKPYKNKSMAQRKAINFDHPDAFD", "aa_hexdigest": "e1ab7c080a2409d72ea9958a36166eff", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00485.24", "name": "PRK", "length": 69, "aa_cov": 0.8823529411764706, "hmm_cov": 1.0, "evalue": 6.67384735076276e-07, "score": 24.76234245300293, "start": 9, "stop": 68 } ], "seq_stats": { "molecular_weight": 7606.822200000001, "isoelectric_point": 9.629658699035645 }, "id": "APKECPOBEN_1209", "locus": "GCA002090215_01209" }, { "type": "cds", "sequence": "contig_1", "start": 682130, "stop": 682375, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00485.24" ], "nt": "ATGGGTAAAACAGTGGCTGTGCCAATGTATGACTATGTTAATTACACCCGTAAAAAGGAAACCATAGAAATAGGTCCTTTAAATGTAGTCATCTTGGAAGGTTTAATGCCGTGGTTTGATGAACGGATTGCTAAATTGTGTAAGCTCAAGATCTTTGTGGAAGCGACTGGAGAGGAACGCTTAATTCGCCGGATTGAACGGGACTGGGAACGCGGTCGTGATGTCGCTTCTATCATTAAACAATGA", "aa": "MGKTVAVPMYDYVNYTRKKETIEIGPLNVVILEGLMPWFDERIAKLCKLKIFVEATGEERLIRRIERDWERGRDVASIIKQ", "aa_hexdigest": "77dc1989918fd2cfa2d3ab0aab7eea23", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00485.24", "name": "PRK", "length": 81, "aa_cov": 0.9876543209876543, "hmm_cov": 1.0, "evalue": 2.7482485522050393e-18, "score": 61.91554260253906, "start": 2, "stop": 81 } ], "seq_stats": { "molecular_weight": 9540.122499999992, "isoelectric_point": 8.816132164001466 }, "id": "APKECPOBEN_1210", "locus": "GCA002090215_01210" }, { "type": "cds", "sequence": "contig_1", "start": 682394, "stop": 682531, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTACGAAATCTTTGTCGAAAAGATGAAACAGAAGGCAGATTTAATTATTCCCTGGAGTGAAAGACACGAAGTGAGTACTAATGTGCTCGACTTTGCAATTGAACACCTCTTTCGCAAACACGTGGATCCCAACTAG", "aa": "MYEIFVEKMKQKADLIIPWSERHEVSTNVLDFAIEHLFRKHVDPN", "aa_hexdigest": "6d882a79fb617e52dbfdf00aca6704cf", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5455.228799999999, "isoelectric_point": 5.80777416229248 }, "id": "APKECPOBEN_1211", "locus": "GCA002090215_01211" }, { "type": "cds", "sequence": "contig_1", "start": 682528, "stop": 683193, "strand": "-", "frame": 1, "gene": "nadE", "product": "NH(3)-dependent NAD(+) synthetase", "db_xrefs": [ "COG:COG0171", "COG:H", "EC:6.3.1.5", "GO:0003952", "GO:0004359", "GO:0005524", "GO:0005737", "GO:0008795", "GO:0009435", "GO:0046872", "SO:0001217", "UniRef:UniRef50_P47623", "UniRef:UniRef90_P75216" ], "nt": "GTGGTTGTTGGATTGTCAGGTGGAGTTGATTCGGCAGTTGTAGCCGCCATGACTAAAAAACTGTTTCCGCAAAACCACTTAACCTTGGTAATGCACATTAATAACTCCGAGTTAGATCACAAGGCCACAACTGCCTTAGTGGAACAATTACAACTCAACAATAAACAGGTGGATTTAGAACCACCTTACCGCGCTATGTTGCAAGCATTGACCATTGATCCCCAAAAGGAGTTAATGGTAGCCGGCAACCTCAAAGCACGTTTACGAATGGCTTGTCTATACACCCATGCCCAAAAGCACAACTACTTAGTTTTGGGTACTGGCAACTTCATTGAGTACAGCTTGGGCTACTTTACTAAATGGGGTGATGGGGCGTGTGATGTGGCACCCTTAGCGTTCTTGTTAAAGAGCGATGTTTATGCTTTATCCCAGCACTTCAATGTTCCTGAATTGGTCATTGAACGAGCCCCTACTGCTAGTCTGTTTGCCGGGCAGACTGACGAAGCGGAAATGGGTTTGACGTATAAGGAATTGGACCAGTACTTTCAGGGCCACTTACAGTTAAGTGCCACGAAACAACAACGGGTTGACCACTTGCGCCAAAGCTCCCAGCACAAGCGTAGTTTACCTAAAACCTTTAAACCACTTTATTCCTTCCAAATCTAG", "aa": "MVVGLSGGVDSAVVAAMTKKLFPQNHLTLVMHINNSELDHKATTALVEQLQLNNKQVDLEPPYRAMLQALTIDPQKELMVAGNLKARLRMACLYTHAQKHNYLVLGTGNFIEYSLGYFTKWGDGACDVAPLAFLLKSDVYALSQHFNVPELVIERAPTASLFAGQTDEAEMGLTYKELDQYFQGHLQLSATKQQRVDHLRQSSQHKRSLPKTFKPLYSFQI", "aa_hexdigest": "494604ac890dec4b640a4f9f3b11fc2d", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75216", "query_cov": 1.0, "subject_cov": 0.8911290322580645, "identity": 0.995, "score": 433.0, "evalue": 3.6e-153, "valid": true, "gene": "nadE", "product": "NH(3)-dependent NAD(+) synthetase", "ec_ids": [ "6.3.1.5" ], "uniref50_id": "UniRef50_P47623", "cog_id": "COG0171", "cog_category": "H", "go_ids": [ "GO:0005737", "GO:0005524", "GO:0004359", "GO:0046872", "GO:0003952", "GO:0008795", "GO:0009435" ] }, "pscc": { "uniref50_id": "UniRef50_P47623", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47623" ], "product": "NH(3)-dependent NAD(+) synthetase" }, "genes": [ "nadE" ], "id": "APKECPOBEN_1212", "locus": "GCA002090215_01212" }, { "type": "cds", "sequence": "contig_1", "start": 683267, "stop": 684433, "strand": "-", "frame": 3, "gene": "obgE", "product": "GTPase ObgE", "db_xrefs": [ "COG:COG0536", "COG:DL", "EC:3.6.5.-", "GO:0000287", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0042254", "SO:0001217", "UniRef:UniRef50_P75215", "UniRef:UniRef90_P75215" ], "nt": "GTGTTGCAAGCCGACCACAACTGCGATTCCCTCTTTTTTTTAAAGAATAAAAAACACCTGTTTGCTGAAAGTGGCGGCAATGGCAAACCCGATTTAGCCCATGGTAAAAACGGGGAAGATTTGGTTATTAAGGTACCAGTGGGAACTACGGTGCGTGACCTTGATACTAACCAGATCTTAATGGACTTTGTTCATGACCAACAGAGCTTTATTCTCTGTTACGGTGGCAAGGGCGGTAAGGGCAACGCAGCTTTTAAAAGTCCAATTATGCGCGCTCCCAATCTGTATGAAAACGGCGACAAGGGTCAAAGTTTGCATGTGAGCTTGGAAATAAAGTATTTAGCTAATGTGGGGATTGTGGGTTTTCCCAACACCGGTAAGTCCACTTTAATTTCCAAACTGTCCAACGCTAAACCCAAGATTGCTAACTACCGTTTTACAACCTTAGTGCCCGTATTGGGGGTAGTTAAACACAACGACCAAAGTTTGGTGTTTGCTGATATTCCCGGGTTAATTGAAAACGCCAGTGAGGGTAGTGGTCTGGGTCACTATTTCCTGCGCCACATTGAGCGTTGTGAAATCCTAATTCACTTAATTTCACTCGATCCCGTGGATCATGACGATCCTTGTCAAGCGTATGAACAAATTATGCGTGAACTGTCGAAGTACTCCCAGCTGTTAGTTAAAAAGAAGATGCTGGTCGTGGCCAATAAAACAGACGTTGACCTTGATGGTACCCGTTTTCAAAAGTTAGCACAATACCTCGAAAATAAAGGAATTCCCTTGTTCAAAATTAGTGCACTCAAACAAGAACTGGGTGATCTAGTGGCCCAAGTGTTTGCGCTCCACCAAAAAACGCTAGCGCAATTCGGTGCCAATAAGTTTCATTTACCCATGGAAATGGAGAAGCACTATGTCTTTGAACAGGCCAGTGAAACCGACCATGATCCACTCAACATTGAACGTGATGCCTTGGGGCGGTGGCATGTTGAATGCAAGCGTTTACATTATTGGTTTGACAAGATTCCCCAAACTACACTTGACAACATTCGCCGTTTAGGTAATAAGATTAAAGAAGTGGGCATAGAAGACCAATTAAAGGTGGCAGGTGCCAAAAAAGGCGATGTCATTGTGTTTGCTGGCCAGGAATTTGTAATTAATGACTAA", "aa": "MLQADHNCDSLFFLKNKKHLFAESGGNGKPDLAHGKNGEDLVIKVPVGTTVRDLDTNQILMDFVHDQQSFILCYGGKGGKGNAAFKSPIMRAPNLYENGDKGQSLHVSLEIKYLANVGIVGFPNTGKSTLISKLSNAKPKIANYRFTTLVPVLGVVKHNDQSLVFADIPGLIENASEGSGLGHYFLRHIERCEILIHLISLDPVDHDDPCQAYEQIMRELSKYSQLLVKKKMLVVANKTDVDLDGTRFQKLAQYLENKGIPLFKISALKQELGDLVAQVFALHQKTLAQFGANKFHLPMEMEKHYVFEQASETDHDPLNIERDALGRWHVECKRLHYWFDKIPQTTLDNIRRLGNKIKEVGIEDQLKVAGAKKGDVIVFAGQEFVIND", "aa_hexdigest": "7c5bbd50a3cee0623c8e3dc979003ce8", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75215", "query_cov": 1.0, "subject_cov": 0.8960739030023095, "identity": 0.997, "score": 785.0, "evalue": 2.08e-286, "valid": true, "gene": "obgE", "product": "GTPase ObgE", "ec_ids": [ "3.6.5.-" ], "uniref50_id": "UniRef50_P75215", "cog_id": "COG0536", "cog_category": "DL", "go_ids": [ "GO:0000287", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0042254" ] }, "pscc": { "uniref50_id": "UniRef50_P75215", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75215" ], "product": "GTPase Obg" }, "genes": [ "obgE" ], "id": "APKECPOBEN_1213", "locus": "GCA002090215_01213" }, { "type": "cds", "sequence": "contig_1", "start": 684772, "stop": 685122, "strand": "-", "frame": 1, "gene": null, "product": "NADP-dependent alcohol dehydrogenase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A645H7Y1", "UniRef:UniRef90_A0AB38W5V0" ], "nt": "GTGGAAAAGGTGTTAATTTCTGGTGGTGGTTCGACTATCTTAGAAACAGCGATTAAGGTTTTAAGACCAGGAGGCAAAATTGGTAACGTTAATTACTTTGGCGCTGGTGAGTTTTTAACTATTCCTAGAGTTGAATGGGGCGTTGGTATGGCTCACAAGGCCATTCATGGTGGTCTTATGCTTGGTGGTCGGTTAAGGTTAGAAAAATTAGCACGTTTAATTATGACCAAAAAACTCGATCCTTCCAAAATGATTACCCATCGCTTTAAGGGTTTTGAACACATTGAAGAAGCCCTGTTTTTAATGAAAGATAAACCGAAGGACTTAATTAAATCCGTGGTTATTTTTTAA", "aa": "MEKVLISGGGSTILETAIKVLRPGGKIGNVNYFGAGEFLTIPRVEWGVGMAHKAIHGGLMLGGRLRLEKLARLIMTKKLDPSKMITHRFKGFEHIEEALFLMKDKPKDLIKSVVIF", "aa_hexdigest": "3dab30da03fae1a49e874498de88cc9e", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002ED5893F", "query_cov": 0.9482758620689655, "subject_cov": 0.8148148148148148, "identity": 0.5820000000000001, "score": 139.0, "evalue": 1.88e-40, "valid": false, "uniref50_id": "UniRef50_A0A352UL42" }, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 684772, "stop": 685392, "observations": { "mutation": [ 685140, 685341, 685350 ], "stop": [ 685140, 685341, 685350 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB38W5V0", "query_cov": 1.0, "subject_cov": 0.5631067961165048, "identity": 1.0, "score": 230.0, "evalue": 2.22e-75, "gene_start": 1, "gene_end": 116, "reference_start": 91, "reference_end": 206, "reference_sequence": "MYVVRHDSHRLSRQWTSWHPIRCRVVVFGAGPVGLMAIAGAALKGAGRIIVVDSRPDIVEIAKAYGATDYIDFKKVSVVDEILKWTNNEGMEKVLISGGGSTILETAIKVLRPGGKIGNVNYFGAGEFLTIPRVEWGVGMAHKAIHGGLMLGGRLRLEKLARLIMTKKLDPSKMITHRFKGFEHIEEALFLMKDKPKDLIKSVVIF" }, "paralog": false, "identity": 0.9757281553398058, "score": 392.0, "evalue": 1.72e-141, "description": "Internal stop codon at 685140, 685341, 685350. Nonsense mutation around 685140, 685341, 685350.", "psc": { "uniref90_id": "UniRef90_A0AB38W5V0", "product": "NADP-dependent alcohol dehydrogenase", "uniref50_id": "UniRef50_A0A645H7Y1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB38W5V0", "UniRef:UniRef50_A0A645H7Y1" ] } }, "id": "APKECPOBEN_1214", "locus": "GCA002090215_01214" }, { "type": "cds", "sequence": "contig_1", "start": 685138, "stop": 685287, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00107.32" ], "nt": "ATGGCGATCGCTGGTGCAGCCTTAAAGGGTGCTGGACGCATTATTGTGGTGGATTCTAGACCAGATATTGTGGAAATTGCCAAAGCATACGGTGCGACTGATTACATTGATTTCAAAAAGGTTTCAGTTGTGGATGAAATCTTGAAGTGA", "aa": "MAIAGAALKGAGRIIVVDSRPDIVEIAKAYGATDYIDFKKVSVVDEILK", "aa_hexdigest": "f8c5e57a2fa40ab36b66ca698a395dbf", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00107.32", "name": "ADH_zinc_N", "length": 48, "aa_cov": 0.9795918367346939, "hmm_cov": 0.9791666666666666, "evalue": 1.5842113344327445e-09, "score": 33.29688262939453, "start": 1, "stop": 48 } ], "seq_stats": { "molecular_weight": 5192.0359, "isoelectric_point": 6.049965095520019 }, "id": "APKECPOBEN_1215", "locus": "GCA002090215_01215" }, { "type": "cds", "sequence": "contig_1", "start": 685287, "stop": 685526, "strand": "-", "frame": 2, "gene": null, "product": "NADP-dependent alcohol dehydrogenase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B0EC62", "UniRef:UniRef90_A0AB38W6U1" ], "nt": "ATGCACTCCGGTGGCATGCTGGGTGGTTGGAAGTTTTCCAACGTTAAAGATGGCATGTTTGCTGAATACTTTCACGTCAATGATGCTGAGGGTAACTTAGCATTGATGCCAGAAGGGATGGATTTAGCTGATGCATGTATGTTGTCAGACATGATTCCCACAGGCTTTCACGCCAATGAACTAGCTGACATCCAATACGGTGTCGCGTTGTCGTTTTTTGTGCTGGTCCAGTTGGTTTAA", "aa": "MHSGGMLGGWKFSNVKDGMFAEYFHVNDAEGNLALMPEGMDLADACMLSDMIPTGFHANELADIQYGVALSFFVLVQLV", "aa_hexdigest": "7342993a5f61ef62f56f2f82cdc05774", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 685287, "stop": 685826, "observations": { "mutation": [ 685559, 685787 ], "stop": [ 685559, 685787 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB38W6U1", "query_cov": 1.0, "subject_cov": 0.441340782122905, "identity": 0.987, "score": 161.0, "evalue": 3.45e-49, "gene_start": 1, "gene_end": 79, "reference_start": 101, "reference_end": 179, "reference_sequence": "MKAYAMLKIGATGWIEKPRPVCGPNDAIIRPLAVAPCTSDVHTVWEGGIGERHNMVLGHEGCGVVDEVGSEVKSFKVGDRVLVAAITPEWNSVNAQAGYPMHSGGMLGGWKFSNVKDGMFAEYFHVNDAEGNLALMPEGMDLADACMLSDMIPTGFHANELADIQYGVALSFLVLVQLV" }, "paralog": false, "identity": 0.9832402234636871, "score": 360.0, "evalue": 9.87e-130, "description": "Internal stop codon at 685559, 685787. Nonsense mutation around 685559, 685787.", "psc": { "uniref90_id": "UniRef90_A0AB38W6U1", "product": "NADP-dependent alcohol dehydrogenase", "uniref50_id": "UniRef50_B0EC62", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB38W6U1", "UniRef:UniRef50_B0EC62" ] } }, "id": "APKECPOBEN_1216", "locus": "GCA002090215_01216" }, { "type": "cds", "sequence": "contig_1", "start": 685557, "stop": 685664, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08240.17" ], "nt": "ATGGTTTTAGGCCATGAGGGCTGTGGTGTTGTGGATGAAGTGGGGTCTGAGGTCAAAAGCTTTAAGGTTGGTGATCGCGTTTTAGTGGCAGCCATTACACCAGAATGA", "aa": "MVLGHEGCGVVDEVGSEVKSFKVGDRVLVAAITPE", "aa_hexdigest": "028f5933663f10cf6e1ec59bc2a80dd0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08240.17", "name": "ADH_N", "length": 32, "aa_cov": 0.9142857142857143, "hmm_cov": 1.0, "evalue": 5.0482809906745806e-11, "score": 38.2827033996582, "start": 1, "stop": 32 } ], "seq_stats": { "molecular_weight": 3628.1351999999997, "isoelectric_point": 4.781830406188964 }, "id": "APKECPOBEN_1217", "locus": "GCA002090215_01217" }, { "type": "cds", "sequence": "contig_1", "start": 686106, "stop": 686273, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTAGTGTTGGTAGTGTTAATGCTGAGTTTCTTTGTAATTAATAAACTAACTAACTTAGTTTTACTACGTTCACGCTATGCCATGGTAGTAGCACAAGGACTGGTTCACACAGGTAAAGTCAAACAACAAAGCCAACAGGCTATGTCTGACATTAAAACGAAATGA", "aa": "MLVLVVLMLSFFVINKLTNLVLLRSRYAMVVAQGLVHTGKVKQQSQQAMSDIKTK", "aa_hexdigest": "87bf19a1e80e57d93829103f5e0d3f66", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6190.500700000004, "isoelectric_point": 10.65547962188721 }, "id": "APKECPOBEN_1218", "locus": "GCA002090215_01218" }, { "type": "cds", "sequence": "contig_1", "start": 686301, "stop": 686594, "strand": "-", "frame": 2, "gene": null, "product": "MPN565 family protein", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P75213", "UniRef:UniRef90_P75213" ], "nt": "TTGGTCTTTGTCTACTACATCATTGTGTGGGTGTTATTAGGGGAAATTAATGCCTTAGGTTTTAACTTTGTCATTGCCCCAAATAACCGTACTTTAATGGGTAGGGAAGTAGAACCCATTTTTGATTTAGTACTACTGTGGTTCTTACTAGTGCCCTTAGTAGTTTATGCGTTGCTTTTACTGCTTTTATTCTTTACAACTCCATACCTAATTGTGGAGGCAATTCCCTTTTGTTATGGAATTGCTTTGATGATGATTAGCCTCTTTATGAGTGGTATGTTTCCTCAAGCTTGA", "aa": "MVFVYYIIVWVLLGEINALGFNFVIAPNNRTLMGREVEPIFDLVLLWFLLVPLVVYALLLLLLFFTTPYLIVEAIPFCYGIALMMISLFMSGMFPQA", "aa_hexdigest": "2661d68f16c021f36a56fa4daaceb78f", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 686040, "stop": 686666, "observations": { "mutation": [ 686108, 686303, 686621 ], "stop": [ 686108, 686303, 686621 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75213", "query_cov": 1.0, "subject_cov": 0.46634615384615385, "identity": 0.9790000000000001, "score": 162.0, "evalue": 8.7e-49, "gene_start": 1, "gene_end": 97, "reference_start": 25, "reference_end": 121, "reference_sequence": "MEQFFQSTAKRKTPAWLTGSFSFFLVFVYYIIVWVLLGEINALGFNFVIAPNNRTLMGREVEPIFDLVLLWFLLVPLVVYALLLLLLFFTTPYLIVEAIPFFYGIALMMISLFMSGMFPQAWNVWVIFGRFVLVLVVLMLSFFVINKLTNLVLLRSRYAMVVAQGLVHTGKVKQQSQQAMSDIKTKWDKEKSKTVVVTIKKKRVKSSD" }, "paralog": false, "identity": 0.9759615384615384, "score": 373.0, "evalue": 3.66e-134, "description": "Internal stop codon at 686108, 686303, 686621. Nonsense mutation around 686108, 686303, 686621.", "psc": { "uniref90_id": "UniRef90_P75213", "product": "MPN565 family protein", "uniref50_id": "UniRef50_P75213", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75213", "UniRef:UniRef50_P75213", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75213", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75213" ], "product": "Uncharacterized protein MG384.1 homolog" } }, "id": "APKECPOBEN_1219", "locus": "GCA002090215_01219" }, { "type": "cds", "sequence": "contig_1", "start": 686668, "stop": 687381, "strand": "-", "frame": 1, "gene": "ugpQ", "product": "putative MG385-like protein", "db_xrefs": [ "COG:COG0584", "COG:I", "GO:0006629", "GO:0008081", "RefSeq:WP_010874923.1", "SO:0001217", "UniParc:UPI000013970F", "UniRef:UniRef100_P75212", "UniRef:UniRef50_P47625", "UniRef:UniRef90_P75212" ], "nt": "ATGCGCAAACAGTTTTTAATTGCACACCGTGGTTACAGCACCATTGCTCCTGAAAACACTCATTTAGCCTTTGCTGCTGCACAATTGTTTGGTTTTGATGGTTTGTGGATGGAAGTGCAACTCACTAAAGATAAACAAATAGTAGTCACTAATTTAGACAACTATAAGGTAGGAAATAAAACCCATAAACTGAGTGATATTAACCTAGTTAATCTCAAAAAGATTAACTTAGCGAAGCAGTTTAAGGTAAATGTCCAAGAACAGACAATCTTGACCTTAAAAGAGGTGTTAATGGAATTTTTAACCCCGTTTCGCTTTCTCTTGCTTTTTATTAAGGGCGAAGAAGAACAACAAAACCAGGTACTAGTAGAACAGTTAGCACAACTACTAGAAGGTTTTGAGTTAGCTAAAGAAAAGTTAATCTTGCTGTCCAGTCACTTTTCCACGATTAAGTACCTCAACGAAAAGTTAAAGAGCTTTAAAACTAGTTTTGTCTTTAACAGTAAAAAGCAGTTAGTTCATTTAACTAAAGATGAGATTACCCAGAACTGTCGTTTCTTAGCGCCCAATGATAGCTTTTACCACAAAAATTGTGCGGAGCTACAAAGCTATGGCCTGCCAATTATCCTCTGGGTAATTAAAGGGCTTTTACGTTACCAGTTTTACGAAAACGATCGCTTTGTTAAATTTCAAATAGCAGCACAACTTTACTAA", "aa": "MRKQFLIAHRGYSTIAPENTHLAFAAAQLFGFDGLWMEVQLTKDKQIVVTNLDNYKVGNKTHKLSDINLVNLKKINLAKQFKVNVQEQTILTLKEVLMEFLTPFRFLLLFIKGEEEQQNQVLVEQLAQLLEGFELAKEKLILLSSHFSTIKYLNEKLKSFKTSFVFNSKKQLVHLTKDEITQNCRFLAPNDSFYHKNCAELQSYGLPIILWVIKGLLRYQFYENDRFVKFQIAAQLY", "aa_hexdigest": "aa94bb1fbf20169247fe5c767527eeee", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013970F", "ncbi_nrp_id": "WP_010874923.1", "uniref100_id": "UniRef100_P75212", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013970F", "RefSeq:WP_010874923.1", "UniRef:UniRef100_P75212" ] }, "ips": { "uniref100_id": "UniRef100_P75212", "uniref90_id": "UniRef90_P75212" }, "psc": { "uniref90_id": "UniRef90_P75212", "gene": "UgpQ", "product": "Uncharacterized protein MG385 homolog", "uniref50_id": "UniRef50_P47625", "cog_id": "COG0584", "cog_category": "I", "go_ids": [ "GO:0008081", "GO:0006629" ] }, "pscc": { "uniref50_id": "UniRef50_P47625", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47625" ], "product": "Uncharacterized protein MG385" }, "genes": [ "ugpQ" ], "id": "APKECPOBEN_1220", "locus": "GCA002090215_01220" }, { "type": "cds", "sequence": "contig_1", "start": 687777, "stop": 687893, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTCATTATGAACCTAGTGGGGAATGGGTATGAGCTGGTTACTTTGATGACAACCAAATATGAACACCAGATGCTAGTGTTGAATGGGCACGTGAAAGTGATTACACAGATCTAA", "aa": "MVIMNLVGNGYELVTLMTTKYEHQMLVLNGHVKVITQI", "aa_hexdigest": "8f59a77be459679adb3303dbbee94c4d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4332.179400000001, "isoelectric_point": 6.696679115295409 }, "id": "APKECPOBEN_1221", "locus": "GCA002090215_01221" }, { "type": "cds", "sequence": "contig_1", "start": 687919, "stop": 688323, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAAACGATTCCTGAACCAGAATCAATTGAAACTACTGAACCAGAACCTAATTTTGAACCAGAAGTAGAGCTTGAACCAGAAATTGAACCTAATTTCGAATCCGAAACTGAAGTTCAACAAGAGCTAGCACAAGAATCAAGTTTCGAGTCTGAACCAGAACCTAATTTTGAAACAGAAGTAGAAGTTCAACCTGAATCAGAAATTGAATCTAAATTCGAAGCCGAAGTTCAATCAGAGCCAAAAGTAAGTTTGAATTCAGATTTTGAAACTAAACCAGAAGCTCAAGCGGAGGTAACGCCAGAAACCTTGGAAGTAGAGGCAACATCTGAAGCTCCAGAACTTCAACCAGAAACTGAAGCAACAAAAGTAGTTGATGACGTTGAAGAAGAGCAACTTGATTGA", "aa": "METIPEPESIETTEPEPNFEPEVELEPEIEPNFESETEVQQELAQESSFESEPEPNFETEVEVQPESEIESKFEAEVQSEPKVSLNSDFETKPEAQAEVTPETLEVEATSEAPELQPETEATKVVDDVEEEQLD", "aa_hexdigest": "d8f2ab916f976f8e3934b32a61c4edfd", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15155.808399999993, "isoelectric_point": 4.0500284194946286 }, "id": "APKECPOBEN_1222", "locus": "GCA002090215_01222" }, { "type": "cds", "sequence": "contig_1", "start": 688501, "stop": 689748, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF16713.10" ], "nt": "GTGTGGAAGGGTTTCTTTGACACCAAACTCAATTGGATTCCTGACCCAGTGCGCTTTACCTTGCCAATGGTTGAAAAAGCCACTACAACTACACCTGTTGTACAAATTGAATTACCAGCACCACCAACTGTAACTGTAGTAGACCAAACATCACCACCAACTGCTGCTGTCACCGTTTCTACATCACAGCCAGTCATAGAAGAGCAGACCACTGTATTTAATCAAACTACTCAGCTAGAACAACTAAGTGTTAGCGCACCACTGTTAGACCAATCGGAAGTGGAAACGGAAATGGTAGAAGTGCCGTTTGTTGCACCGTCAACAACTACCACTCAACCTCAAGTGGTTACTGTACAAGCACAACCAGCATCAAGTTCGATTCAGTTTCAAGAACCAATTATTAAGGTTGAGTTTGTTAATGAAAGTTTTGATTTCAAAAAACCAAGCCAAACAGCAGCTGCAGCTAGTCAAGCCCCTTCTCAAGCAATTAACATTGCTTTAAATGAAGCTGACCTCATTGATGAATTAGTGGCGGTTGGCACCACAGCCACCACAGCCTTACCACAAAGCGAGCTGATTCAAGAGGTGGTGGTGATTGACAACGGTCAACCCCAACAAGCTGGGTTTCATTATGTAGTTGATTTTCTCACTAGCACTGCACCACTAACTGTAGCAGAAATAGAGTTGCAAGAGCAAGAGCTAGTCAATGAGTTTGTCACAACTACTAGTAGAGAAACAACTACTTTTGCATCAACTCCAGTTTTTGAACCAGTTGTAATTCCAACAGTAGAATCTGAAGAACAACTTTTAGAAAACGAGTTTGTTGAATCAACTGTTGTAAGTGCAACATCAAATGAACCTAATGTTGCATCAACTCCAGTTGTTGAAACGGTTGAACTTACAGAAACACCAGTAAGTTTAGAACCGCTTGAAACTGTTCAACTAGAAACAGCACCAGTTGTTACTGAAACTGTTACAGTTACTGAGAAAGCAGTAGAACCAGAAGTATTAGCAGTTGTTGAAGAAGCACCACTTGCTGTAGAACCAATTGTTGAAACTTCAACAACATTAGCTGCAGAGACCGTTGAAGAAGCGCAAGTTGAACAAGAGTCAACAGCTGTTGCAGTCGAACCAGCTATTGAAACCGAGTCAAAAGCAACTTCTGAAGCCCAAGCTGAACTGGATTGGGAGGCGTTAATTGGTAACAGTGAATACGGTTACTTTGATGCAGAGCAAAACTGGATTTGA", "aa": "MWKGFFDTKLNWIPDPVRFTLPMVEKATTTTPVVQIELPAPPTVTVVDQTSPPTAAVTVSTSQPVIEEQTTVFNQTTQLEQLSVSAPLLDQSEVETEMVEVPFVAPSTTTTQPQVVTVQAQPASSSIQFQEPIIKVEFVNESFDFKKPSQTAAAASQAPSQAINIALNEADLIDELVAVGTTATTALPQSELIQEVVVIDNGQPQQAGFHYVVDFLTSTAPLTVAEIELQEQELVNEFVTTTSRETTTFASTPVFEPVVIPTVESEEQLLENEFVESTVVSATSNEPNVASTPVVETVELTETPVSLEPLETVQLETAPVVTETVTVTEKAVEPEVLAVVEEAPLAVEPIVETSTTLAAETVEEAQVEQESTAVAVEPAIETESKATSEAQAELDWEALIGNSEYGYFDAEQNWI", "aa_hexdigest": "a51161f6ff175cc90a83220ca2f6fc30", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF16713.10", "name": "EAGR_box", "length": 21, "aa_cov": 0.05060240963855422, "hmm_cov": 1.0, "evalue": 2.2276140981555806e-11, "score": 38.744998931884766, "start": 395, "stop": 415 } ], "seq_stats": { "molecular_weight": 44792.19870000012, "isoelectric_point": 4.0500284194946286 }, "id": "APKECPOBEN_1223", "locus": "GCA002090215_01223" }, { "type": "cds", "sequence": "contig_1", "start": 689974, "stop": 690495, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCAAAAACGATAAAAAAACAGAACCCATCGAACACTACATTACAGTACAAAAAGTATTTAGAACAGTCTAAAGAGAAAACTGCTAAAGCTAAAAATAAGGATGTTAGCATCGATGATTTACTCAAGAAGCCCTTCTTAGAGGAGATCAAAACCAATGTACTTAAGAAGAATAAAACTACCAGAGCTAGCACCGCTACCAGAGGTACTTCTAAAGTCAAAAAACAGATAGTTGAATCTTCTATTGACTTTTTTGACGAGAAAAAGCGTGGTGTTTTTATTGTGCCACCAGCTGGTACTAGTGTTATTAACGATGACCGGGATGATAATAAAGCGGTAGAGGAGACTGTTAGCAAAACTGCTATTAGTCAAAATCAACTGGCACATTATGCCAACAGTGAGCTTGTTGAAACCGAACAATTTGAACTAAAACCGGTTGCTTTAGAACATAATCAGGTTCTAACCAGCACCCGCCACAGTCAAGAACGTGAATCGATTTTTGAAAAAGCACAACTCTTTTGA", "aa": "MPKTIKKQNPSNTTLQYKKYLEQSKEKTAKAKNKDVSIDDLLKKPFLEEIKTNVLKKNKTTRASTATRGTSKVKKQIVESSIDFFDEKKRGVFIVPPAGTSVINDDRDDNKAVEETVSKTAISQNQLAHYANSELVETEQFELKPVALEHNQVLTSTRHSQERESIFEKAQLF", "aa_hexdigest": "5a6c18c6b1f7954811de60174a524ec2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19684.043899999997, "isoelectric_point": 9.336520957946778 }, "id": "APKECPOBEN_1224", "locus": "GCA002090215_01224" }, { "type": "cds", "sequence": "contig_1", "start": 690595, "stop": 690975, "strand": "-", "frame": 1, "gene": "era", "product": "GTPase Era", "db_xrefs": [ "COG:COG1159", "COG:J", "EC:3.6.5.-", "GO:0000028", "GO:0003723", "GO:0003924", "GO:0005525", "GO:0005829", "GO:0005886", "GO:0043024", "GO:0070181", "SO:0001217", "UniRef:UniRef50_P47627", "UniRef:UniRef90_P75210" ], "nt": "GTGAAACTACAAGCAAATGAGTTTCGTAAAGATGTTGAATTTTTAGATGCTGACACTGATAACTTTAAGATTTTAGAAGCGCTGCGCGAACAGATCATTAAGTACTGCAGCGAAGAAATTCCCCATGTAGTAAGGCTAGAGATTGTCGATAAGAGTTTTGATCAAGCTAAAAACCTGTTGAAGCTACACCTTTCCATTAGTGTGCCTAAACTTAGTCAAAAAAAAATTATTATTGGCCGTAATGCCCAAATGGTGAAAAAGATCGGGATGCAAATGCGTCAAAAACTACTGGAGTACTATGACTGCAATGTCTTTGTGGAATTGTTTGTAAGGACTTATGACCCTAAACAAAAAAAGTCACCTTATGGCTCTTTAATTTAG", "aa": "MKLQANEFRKDVEFLDADTDNFKILEALREQIIKYCSEEIPHVVRLEIVDKSFDQAKNLLKLHLSISVPKLSQKKIIIGRNAQMVKKIGMQMRQKLLEYYDCNVFVELFVRTYDPKQKKSPYGSLI", "aa_hexdigest": "f967823dbf0c4dafbbd23959c56a2e33", "start_type": "GTG", "rbs_motif": null, "genes": [ "era" ], "truncated": "3-prime", "pseudogene": { "start": 690595, "stop": 691275, "observations": { "mutation": [ 690993 ], "stop": [ 690993 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75210", "query_cov": 1.0, "subject_cov": 0.4329896907216495, "identity": 0.992, "score": 246.0, "evalue": 3.04e-80, "gene_start": 1, "gene_end": 126, "reference_start": 166, "reference_end": 291, "reference_sequence": "MESIRIGVLGLTNAGKSTLVNQLHKANNLLVSPMNNTTLLAVSTNTITHEKQNITFIDVPGFSEKRHSSYELISQEIRKALSGIDVLLLVVRSDQQQKLPLLQTQLQPLKRYRDLTRVLLINNFFDVVLQAEDKQAIVLDFKPQAVLETDLLHFDATSFWNQFNEVKLQANEFRKDVEFLDADTDNFKILEALREQIIKYCSEEIPHVVRLEIVDKSFDQAKNLLKLHLSISVPKLSQKKIIIGRNAQMVKKIGMQMRQKLLEYYDCNVFVELFVRTYDPKQKKSPYGSLI" }, "paralog": false, "identity": 0.995575221238938, "score": 436.0, "evalue": 2.38e-157, "description": "Internal stop codon at 690993. Nonsense mutation around 690993.", "psc": { "uniref90_id": "UniRef90_P75210", "gene": "era", "product": "GTPase Era", "ec_ids": [ "3.6.5.-" ], "uniref50_id": "UniRef50_P47627", "cog_id": "COG1159", "cog_category": "J", "go_ids": [ "GO:0000028", "GO:0003723", "GO:0003924", "GO:0005525", "GO:0005829", "GO:0005886", "GO:0043024", "GO:0070181" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75210", "EC:3.6.5.-", "UniRef:UniRef50_P47627", "COG:COG1159", "COG:J", "GO:0000028", "GO:0003723", "GO:0003924", "GO:0005525", "GO:0005829", "GO:0005886", "GO:0043024", "GO:0070181" ] }, "pscc": { "uniref50_id": "UniRef50_P47627", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47627" ], "product": "GTPase Era" } }, "id": "APKECPOBEN_1225", "locus": "GCA002090215_01225" }, { "type": "cds", "sequence": "contig_1", "start": 690991, "stop": 691470, "strand": "-", "frame": 1, "gene": "era", "product": "GTPase Era", "db_xrefs": [ "COG:COG1159", "COG:J", "EC:3.6.5.-", "GO:0000028", "GO:0003723", "GO:0003924", "GO:0005525", "GO:0005829", "GO:0005886", "GO:0043024", "GO:0070181", "SO:0001217", "UniRef:UniRef50_P47627", "UniRef:UniRef90_P75210" ], "nt": "ATGGAATCGATAAGAATTGGTGTTTTAGGTCTAACCAACGCTGGTAAATCAACTCTCGTTAACCAATTACACAAAGCGAATAACTTGTTGGTTTCACCTATGAACAACACTACGTTGTTGGCGGTTAGCACTAATACTATTACCCATGAAAAGCAAAACATTACCTTCATTGATGTTCCTGGCTTTAGTGAAAAACGTCACAGCTCCTATGAGTTAATTAGTCAAGAGATTCGCAAGGCTTTAAGTGGGATAGATGTCTTGTTGTTAGTGGTGCGCAGTGATCAACAGCAAAAGTTACCCTTGTTACAAACGCAATTACAACCGTTAAAGCGTTACCGTGATTTAACTCGTGTTTTGTTAATTAATAACTTCTTTGATGTTGTTTTGCAAGCGGAGGACAAGCAAGCAATTGTGCTCGATTTTAAACCGCAAGCAGTACTGGAAACTGATCTGCTTCATTTTGATGCCACTTCTTTTTGA", "aa": "MESIRIGVLGLTNAGKSTLVNQLHKANNLLVSPMNNTTLLAVSTNTITHEKQNITFIDVPGFSEKRHSSYELISQEIRKALSGIDVLLLVVRSDQQQKLPLLQTQLQPLKRYRDLTRVLLINNFFDVVLQAEDKQAIVLDFKPQAVLETDLLHFDATSF", "aa_hexdigest": "dfe2d35fec5a8946cc6e9fd10130e6a3", "start_type": "ATG", "rbs_motif": null, "genes": [ "era" ], "truncated": "5-prime", "pseudogene": { "start": 690688, "stop": 691470, "observations": { "mutation": [ 690993 ], "stop": [ 690993 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75210", "query_cov": 1.0, "subject_cov": 0.5463917525773195, "identity": 1.0, "score": 297.0, "evalue": 7.31e-100, "gene_start": 1, "gene_end": 159, "reference_start": 1, "reference_end": 159, "reference_sequence": "MESIRIGVLGLTNAGKSTLVNQLHKANNLLVSPMNNTTLLAVSTNTITHEKQNITFIDVPGFSEKRHSSYELISQEIRKALSGIDVLLLVVRSDQQQKLPLLQTQLQPLKRYRDLTRVLLINNFFDVVLQAEDKQAIVLDFKPQAVLETDLLHFDATSFWNQFNEVKLQANEFRKDVEFLDADTDNFKILEALREQIIKYCSEEIPHVVRLEIVDKSFDQAKNLLKLHLSISVPKLSQKKIIIGRNAQMVKKIGMQMRQKLLEYYDCNVFVELFVRTYDPKQKKSPYGSLI" }, "paralog": false, "identity": 0.9961538461538462, "score": 496.0, "evalue": 1.09e-180, "description": "Internal stop codon at 690993. Nonsense mutation around 690993.", "psc": { "uniref90_id": "UniRef90_P75210", "gene": "era", "product": "GTPase Era", "ec_ids": [ "3.6.5.-" ], "uniref50_id": "UniRef50_P47627", "cog_id": "COG1159", "cog_category": "J", "go_ids": [ "GO:0000028", "GO:0003723", "GO:0003924", "GO:0005525", "GO:0005829", "GO:0005886", "GO:0043024", "GO:0070181" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75210", "EC:3.6.5.-", "UniRef:UniRef50_P47627", "COG:COG1159", "COG:J", "GO:0000028", "GO:0003723", "GO:0003924", "GO:0005525", "GO:0005829", "GO:0005886", "GO:0043024", "GO:0070181" ] }, "pscc": { "uniref50_id": "UniRef50_P47627", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47627" ], "product": "GTPase Era" } }, "id": "APKECPOBEN_1226", "locus": "GCA002090215_01226" }, { "type": "cds", "sequence": "contig_1", "start": 691458, "stop": 691763, "strand": "-", "frame": 2, "gene": "ybeY", "product": "rRNA maturation RNase YbeY", "db_xrefs": [ "COG:COG0319", "COG:J", "EC:3.1.-.-", "GO:0004222", "GO:0004521", "GO:0004540", "GO:0005737", "GO:0006364", "GO:0008270", "GO:0042254", "GO:0046872", "SO:0001217", "UniRef:UniRef50_P47628", "UniRef:UniRef90_P75209" ], "nt": "ATGAAGCGCTTAAACCGGCAGTTTCTCAACCGCAAAGGGGTAACTGATGTGATTAGCATCTGTTACAACGAAAATGAGGCGGGGTTTTCACCAGCCATTGGTGAAATCTTTTTGTGTCCCAAACACATCTTTAAGCAGGCCAAGCAGTTTGGTTGCACACCTTGGTTTTTACTAACACGCAACTTAATCCATGGTTTGCTACACCTGTTTGAGTTTGACCATGAGCAAAGTCTGGCCTTTGAAAGTGTAACGATGTTTTTTCAAGATGAAATTCATGAAACCGTTTTAAAACTATGGAATCGATAA", "aa": "MKRLNRQFLNRKGVTDVISICYNENEAGFSPAIGEIFLCPKHIFKQAKQFGCTPWFLLTRNLIHGLLHLFEFDHEQSLAFESVTMFFQDEIHETVLKLWNR", "aa_hexdigest": "81f26937fc4e70cc3ffaf4e7419feb3e", "start_type": "ATG", "rbs_motif": null, "genes": [ "ybeY" ], "truncated": "3-prime", "pseudogene": { "start": 691458, "stop": 691922, "observations": { "mutation": [ 691769 ], "stop": [ 691769 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75209", "query_cov": 1.0, "subject_cov": 0.6558441558441559, "identity": 1.0, "score": 213.0, "evalue": 1.08e-69, "gene_start": 1, "gene_end": 101, "reference_start": 54, "reference_end": 154, "reference_sequence": "MKPSFSINSNYLFKRFFGKNFEAGIELCLQIIKDSLQLSFQPEFALMIVSPWKMKRLNRQFLNRKGVTDVISICYNENEAGFSPAIGEIFLCPKHIFKQAKQFGCTPWFLLTRNLIHGLLHLFEFDHEQSLAFESVTMFFQDEIHETVLKLWNR" }, "paralog": false, "identity": 0.987012987012987, "score": 314.0, "evalue": 1.21e-111, "description": "Internal stop codon at 691769. Nonsense mutation around 691769.", "psc": { "uniref90_id": "UniRef90_P75209", "gene": "ybeY", "product": "rRNA maturation RNase YbeY", "ec_ids": [ "3.1.-.-" ], "uniref50_id": "UniRef50_P47628", "cog_id": "COG0319", "cog_category": "J", "go_ids": [ "GO:0004222", "GO:0004521", "GO:0004540", "GO:0005737", "GO:0006364", "GO:0008270", "GO:0042254", "GO:0046872" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75209", "EC:3.1.-.-", "UniRef:UniRef50_P47628", "COG:COG0319", "COG:J", "GO:0004222", "GO:0004521", "GO:0004540", "GO:0005737", "GO:0006364", "GO:0008270", "GO:0042254", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_P47628", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47628" ], "product": "Endoribonuclease YbeY" } }, "id": "APKECPOBEN_1227", "locus": "GCA002090215_01227" }, { "type": "cds", "sequence": "contig_1", "start": 692110, "stop": 692295, "strand": "-", "frame": 1, "gene": null, "product": "putative MG389-like protein", "db_xrefs": [ "GO:0016020", "SO:0001217", "UniRef:UniRef50_P47629", "UniRef:UniRef90_P75208" ], "nt": "ATGAAGCAAAAAATTATTGGCCTAACTCTAGCTTTCTTTGTTTTATTCTTAACAGCCGTTGCCATCTTATTTACGGTTAAGGTACAACGATACCTAACTACAAGCTTATGGGCAAAATTGTCTGAACAAACCTTTTTAGTTTTTAAAAACGAGCAGGATGAAGCCCAGTTTAACCAGGTATCTTGA", "aa": "MKQKIIGLTLAFFVLFLTAVAILFTVKVQRYLTTSLWAKLSEQTFLVFKNEQDEAQFNQVS", "aa_hexdigest": "fbf65018700e9ab47c995fe43f42d65b", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 691906, "stop": 692295, "observations": { "mutation": [ 692112 ], "stop": [ 692112 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75208", "query_cov": 1.0, "subject_cov": 0.4728682170542636, "identity": 1.0, "score": 112.0, "evalue": 1.06e-30, "gene_start": 1, "gene_end": 61, "reference_start": 1, "reference_end": 61, "reference_sequence": "MKQKIIGLTLAFFVLFLTAVAILFTVKVQRYLTTSLWAKLSEQTFLVFKNEQDEAQFNQVSWTNFQAETTKKEDKKAFRLYKKKISLEQLENENQQQLFQAVNLSINLKQGWYNITILLPSKALFETVF" }, "paralog": false, "identity": 0.9922480620155039, "score": 248.0, "evalue": 1.7e-86, "description": "Internal stop codon at 692112. Nonsense mutation around 692112.", "psc": { "uniref90_id": "UniRef90_P75208", "product": "Uncharacterized protein MG389 homolog", "uniref50_id": "UniRef50_P47629", "go_ids": [ "GO:0016020" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75208", "UniRef:UniRef50_P47629", "GO:0016020" ] }, "pscc": { "uniref50_id": "UniRef50_P47629", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47629" ], "product": "Uncharacterized protein MG389" } }, "id": "APKECPOBEN_1228", "locus": "GCA002090215_01228" }, { "type": "cds", "sequence": "contig_1", "start": 692297, "stop": 693595, "strand": "-", "frame": 3, "gene": "sunT", "product": "Putative ABC transporter ATP-binding MG390-like protein", "db_xrefs": [ "COG:COG2274", "COG:V", "GO:0005524", "GO:0006508", "GO:0008234", "GO:0016887", "GO:0042626", "GO:0043190", "SO:0001217", "UniRef:UniRef50_P75207", "UniRef:UniRef90_P75207" ], "nt": "ATGCAAACATTACAAACCAAGAGTTTTGTCTACTTTAGCAATAAATCACCTAACCAATTAACCGAGCGTCAGTTTTACCTCAAGGAAGTGTTGTCCTTCTTTAATGTCCATATTCCTAACTTAATTATTAGCTGTACAGTAGCTTTAATTATTGGCACCTTAATTGGTATTAACCAAATGGAGTTCTTATGGATAGCAATTGTCCAAATTGTGGTGAATTGTGCCATTTTTCTCTATGACTTCTTTTTCACTAAACGGATTACGAAGCAAGCAATTCCGCAAATGGAGTTACAAAACAAGGTCAGTTTGCAGTTAGATGGCAATTTGCGTGATGAACAAAACGGCAAGCGCTTCAATTACTTAATGATGCAGTTGCGCAAAGCACTTATTAAAAACCAAAACATTAGTAATCAAAAGGAAGTGAACCACTTAGCGAGTGATGGTGTTAAAAGTTTTGCCCAACAGGTATTTGACTTTTTAATTTTGGCCTTGGGCATTATTGGCATTATTGAACAGCGCTACACCTTAGCCTTTCTCTTTTATATCTTTTCCATTCAGGCATTGTTTTCCGCTTATGCCACGAGAATCATCCAGTTTGGTGCAGCAGTTAACCTGTACCAGTTCTGTAAGGACAAACTAGTTACCTTGTTTGAAGACAAGGTTAATGATTGCAATTTCAAGGTCAGTTGGAAGTGTCCCAAAGTGATTAACTTAAACAACTGTAGTATCACTCTGAACCAAAATCTCGATTTGGCTAACCTCAACCTGAATTTAACTAATGGCATGGTTATTAGTGGTGAGAATGGTAGCGGTAAAAGTACCTTGCTGAAAATTCTGACTGGTCGAGGTTTGTCATACCAGGGTCAAATTAAGTTGGATGAATTAGACCTAAAGGATTTTAGTGCAAGCCAACTGTTTCACAACGTTTACTATTTAACGGGGCAGTTAACGGCCTACAACGACATTACTGACTTTGGTTACAGTGAAGCCTTGTTAAACTGTAAAAATCCCCAAGTATACCAGCTGTTAGCCGACACCGGCATCCACAACCAGATTAAGTTAAGTTCAGGACAAAAACAGATCTTGCAACTGTTCTTATTACAAAACCTCAAAGACAAGGTGATTTTGTTAGATGAAACACTCAACGCCATTGCCACGGAGCTCAAACCGCGCGTGTATCAGCTTTTGATCAAACCGTTGACCTATAACAACTTTGTTCTGATGGTCGAACACGATTTACGCTTTGTAAACAGTGAGCAAGACCTAATTAACCTCAGTCCTTATTTACAGCAGACATAA", "aa": "MQTLQTKSFVYFSNKSPNQLTERQFYLKEVLSFFNVHIPNLIISCTVALIIGTLIGINQMEFLWIAIVQIVVNCAIFLYDFFFTKRITKQAIPQMELQNKVSLQLDGNLRDEQNGKRFNYLMMQLRKALIKNQNISNQKEVNHLASDGVKSFAQQVFDFLILALGIIGIIEQRYTLAFLFYIFSIQALFSAYATRIIQFGAAVNLYQFCKDKLVTLFEDKVNDCNFKVSWKCPKVINLNNCSITLNQNLDLANLNLNLTNGMVISGENGSGKSTLLKILTGRGLSYQGQIKLDELDLKDFSASQLFHNVYYLTGQLTAYNDITDFGYSEALLNCKNPQVYQLLADTGIHNQIKLSSGQKQILQLFLLQNLKDKVILLDETLNAIATELKPRVYQLLIKPLTYNNFVLMVEHDLRFVNSEQDLINLSPYLQQT", "aa_hexdigest": "41023850ad6d8426abc7562c5c088333", "start_type": "ATG", "rbs_motif": null, "genes": [ "sunT" ], "truncated": "3-prime", "pseudogene": { "start": 692297, "stop": 693895, "observations": { "mutation": [ 693601, 693628 ], "stop": [ 693601, 693628 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75207", "query_cov": 1.0, "subject_cov": 0.6545454545454545, "identity": 1.0, "score": 825.0, "evalue": 7.89e-298, "gene_start": 1, "gene_end": 432, "reference_start": 229, "reference_end": 660, "reference_sequence": "MKIIYQEQPNECGICVLGMLANELHEDKYAHDELLEQINLPASGLSFFELETYGKKFGLEIASYQLTLEELKQLEGKYFIVHFPKHFVVVHKKQDNLWEVFDPAKGKYLLNDEELKKQWTGYAATVQKSFKEIPPINKRNFFKHFFDLNLIIFYVFIELIIIGISTLLATASKTMIANTVDFGTSVNIVVFVVFFLVLKGLYLLLYALLQMVRNVLFWKQYRGYLGWIMQTLQTKSFVYFSNKSPNQLTERQFYLKEVLSFFNVHIPNLIISCTVALIIGTLIGINQMEFLWIAIVQIVVNCAIFLYDFFFTKRITKQAIPQMELQNKVSLQLDGNLRDEQNGKRFNYLMMQLRKALIKNQNISNQKEVNHLASDGVKSFAQQVFDFLILALGIIGIIEQRYTLAFLFYIFSIQALFSAYATRIIQFGAAVNLYQFCKDKLVTLFEDKVNDCNFKVSWKCPKVINLNNCSITLNQNLDLANLNLNLTNGMVISGENGSGKSTLLKILTGRGLSYQGQIKLDELDLKDFSASQLFHNVYYLTGQLTAYNDITDFGYSEALLNCKNPQVYQLLADTGIHNQIKLSSGQKQILQLFLLQNLKDKVILLDETLNAIATELKPRVYQLLIKPLTYNNFVLMVEHDLRFVNSEQDLINLSPYLQQT" }, "paralog": false, "identity": 0.9962406015037594, "score": 1035.0, "evalue": 0.0, "description": "Internal stop codon at 693601, 693628. Nonsense mutation around 693601, 693628.", "psc": { "uniref90_id": "UniRef90_P75207", "gene": "SunT", "product": "Putative ABC transporter ATP-binding MG390 homolog", "uniref50_id": "UniRef50_P75207", "cog_id": "COG2274", "cog_category": "V", "go_ids": [ "GO:0043190", "GO:0005524", "GO:0016887", "GO:0042626", "GO:0008234", "GO:0006508" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75207", "UniRef:UniRef50_P75207", "COG:COG2274", "COG:V", "GO:0043190", "GO:0005524", "GO:0016887", "GO:0042626", "GO:0008234", "GO:0006508" ] }, "pscc": { "uniref50_id": "UniRef50_P75207", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75207" ], "product": "Putative ABC transporter ATP-binding MG390 homolog" } }, "id": "APKECPOBEN_1229", "locus": "GCA002090215_01229" }, { "type": "cds", "sequence": "contig_1", "start": 693626, "stop": 693904, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCAAAAATCATTTAAAGAAATTCCGCCCATTAATAAGCGCAATTTCTTTAAGCACTTCTTTGATTTAAACCTGATTATTTTCTATGTCTTCATTGAACTAATCATTATTGGCATTTCAACACTTTTAGCAACGGCCTCTAAAACGATGATTGCCAACACCGTTGACTTTGGGACATCAGTTAACATTGTCGTTTTTGTGGTTTTCTTTTTAGTTTTAAAAGGCTTGTACTTACTTTTGTATGCCTTGTTGCAAATGGTGCGTAATGTTTTGTTTTGA", "aa": "MQKSFKEIPPINKRNFFKHFFDLNLIIFYVFIELIIIGISTLLATASKTMIANTVDFGTSVNIVVFVVFFLVLKGLYLLLYALLQMVRNVLF", "aa_hexdigest": "7666c791d5f5341b6690e0381e40269d", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10638.804399999994, "isoelectric_point": 9.69728603363037 }, "id": "APKECPOBEN_1230", "locus": "GCA002090215_01230" }, { "type": "cds", "sequence": "contig_1", "start": 693923, "stop": 694279, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAATTATTTATCAAGAACAACCCAACGAATGTGGCATTTGTGTGCTTGGCATGTTAGCCAATGAACTGCATGAGGATAAGTATGCCCATGATGAACTGTTGGAACAAATTAACCTACCAGCTAGTGGTTTGAGTTTCTTTGAACTAGAGACCTATGGCAAGAAGTTTGGTCTCGAAATTGCTAGTTACCAGCTAACCCTAGAAGAGCTCAAACAACTAGAGGGTAAATACTTTATTGTCCACTTCCCTAAGCATTTTGTGGTGGTGCATAAAAAACAAGATAATCTCTGGGAAGTATTTGACCCCGCTAAAGGGAAATATCTTTTAAATGATGAAGAATTAAAGAAACAGTGA", "aa": "MKIIYQEQPNECGICVLGMLANELHEDKYAHDELLEQINLPASGLSFFELETYGKKFGLEIASYQLTLEELKQLEGKYFIVHFPKHFVVVHKKQDNLWEVFDPAKGKYLLNDEELKKQ", "aa_hexdigest": "ce8098bad00b7f2b0d6cabca416d3d6c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13817.719499999987, "isoelectric_point": 5.183454704284666 }, "id": "APKECPOBEN_1231", "locus": "GCA002090215_01231" }, { "type": "cds", "sequence": "contig_1", "start": 694383, "stop": 694613, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00883.26" ], "nt": "ATGCACCCGGATTACCTAAAACCATTGGAATCAAAACTAGCGGACTTACAAAACTCCACTAGTGTTAAGGGAGCAGGTTCATCCCGGGCGGCATGTTTCTTAGCTGAATTTAGGGAAGGGGTACCTTTGATCCACTGTGATATTGCATCCACAGCTTCGATTCAAGATCTTGGTCAAGGGGTTTTAGTACGTACTTTATACGAACGTGCTGCGCAGCAAGCAAAGGAATAA", "aa": "MHPDYLKPLESKLADLQNSTSVKGAGSSRAACFLAEFREGVPLIHCDIASTASIQDLGQGVLVRTLYERAAQQAKE", "aa_hexdigest": "4fbf813e5ddb1cf735b4bca6149ef76a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00883.26", "name": "Peptidase_M17", "length": 73, "aa_cov": 0.8552631578947368, "hmm_cov": 0.9863013698630136, "evalue": 4.023832339343207e-12, "score": 41.44631576538086, "start": 2, "stop": 66 } ], "seq_stats": { "molecular_weight": 8203.218999999997, "isoelectric_point": 6.064061164855956 }, "id": "APKECPOBEN_1232", "locus": "GCA002090215_01232" }, { "type": "cds", "sequence": "contig_1", "start": 694623, "stop": 695720, "strand": "-", "frame": 2, "gene": "pepA", "product": "leucyl aminopeptidase", "db_xrefs": [ "COG:COG0260", "COG:E", "EC:3.4.11.1", "GO:0005737", "GO:0006508", "GO:0030145", "GO:0070006", "SO:0001217", "UniRef:UniRef50_P47631", "UniRef:UniRef90_P75206" ], "nt": "ATGAAATTAAATAAGTTATTTGACAGCAAGACGGTTGTTTTTAAAAAGTCCGATAAGTACGGCAAGAGTGATTGCTGTAAGACTAAGTGTGTTGAAGGCCAAATTGAATTTGGCGTAAAAATTCCGACTGATTTCCCCGCTTTCAACCGCGCTCTAGTTACCTTTTTGAAAACGCAAAAGAATCAACTAAACGTGGACTTAGACAGTTTTGTGGAACTCTACAAAGCAGAAAACCTCTGTTACAAAACAGCATTAAAGGTAGTGGTAGCTAGCATTACCTTCTGTGAAACAACACCTTTCACCATGAAGACCGAACCACAAAAGAATGTGGAAGTAGCAGTGAAGTGTGACAGCGAACACACGAGCCTCATTAAAGAATACGAGGTAGTGGGTAACTATGTCAACATGGCGCGTCAACTCCAAGACACCCCCTCTGACCAACTCTACCCAGAGGAATTTGTGAAGCGCTTTGAAAAGGCGGCTACTGGTTTAGGTGTTAAGATTACCGTTTTAAAGCAAGCTGATCTCATCAAAAAGAAGATGGGCTTACTGTTAGGGGTTAACAAGGGTTCGGAACGTGAAGCGCGTTTACTAGTAATTTCCTATAACAATAATAAGAAGAGCAGTGAAACTTTAGCTTTAGTTGGTAAGGGCATTACCTATGATTCGGGTGGGATGAACATTAAGACCGGTGACTACATGCGTGGTATGAAGTACGACATGAGTGGGGCAGCTATTGTGTGTTCCACCGTGTTGGCCTTAGCGAAAAACAAGGTGAAAACCAATGTAGTAGCAGTAGCTGCTTTAACAGAAAACCTACCAGGTCCCCACGCACAACGCCCTGATGACATCCAAACCGCTTACAATGGTAAAACGGTTGAAATTGACAATACCGATGCTGAAGGTCGCTTGGTGTTAGCCGATGCCATTAGCTATGCTGCTAAGGATCTTAAAGCTACCCAAATTATTGATGTCGCTACCTTAACCGGTTTAATGTCTTACATCTTAAGTACCACTTACACCGGGATCTTTAGTACCTGTGACATGGCATGGGATGCCTTTAAAAAAGCAGCTTGCTGTGCAGGCGAACCGGTATGA", "aa": "MKLNKLFDSKTVVFKKSDKYGKSDCCKTKCVEGQIEFGVKIPTDFPAFNRALVTFLKTQKNQLNVDLDSFVELYKAENLCYKTALKVVVASITFCETTPFTMKTEPQKNVEVAVKCDSEHTSLIKEYEVVGNYVNMARQLQDTPSDQLYPEEFVKRFEKAATGLGVKITVLKQADLIKKKMGLLLGVNKGSEREARLLVISYNNNKKSSETLALVGKGITYDSGGMNIKTGDYMRGMKYDMSGAAIVCSTVLALAKNKVKTNVVAVAALTENLPGPHAQRPDDIQTAYNGKTVEIDNTDAEGRLVLADAISYAAKDLKATQIIDVATLTGLMSYILSTTYTGIFSTCDMAWDAFKKAACCAGEPV", "aa_hexdigest": "fdd1ae83e24b3cd833bd1a60de804876", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75206", "query_cov": 1.0, "subject_cov": 0.8202247191011236, "identity": 1.0, "score": 709.0, "evalue": 9.43e-257, "valid": true, "gene": "pepA", "product": "leucyl aminopeptidase", "ec_ids": [ "3.4.11.1" ], "uniref50_id": "UniRef50_P47631", "cog_id": "COG0260", "cog_category": "E", "go_ids": [ "GO:0005737", "GO:0030145", "GO:0070006", "GO:0006508" ] }, "pscc": { "uniref50_id": "UniRef50_P47631", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47631" ], "product": "Probable cytosol aminopeptidase" }, "genes": [ "pepA" ], "id": "APKECPOBEN_1233", "locus": "GCA002090215_01233" }, { "type": "cds", "sequence": "contig_1", "start": 695734, "stop": 697365, "strand": "-", "frame": 1, "gene": "groL", "product": "chaperonin GroEL", "db_xrefs": [ "COG:COG0459", "COG:O", "EC:5.6.1.7", "GO:0005524", "GO:0005737", "GO:0016853", "GO:0042026", "GO:0051082", "GO:0140662", "RefSeq:WP_010874930.1", "SO:0001217", "UniParc:UPI00001276EF", "UniRef:UniRef100_P78012", "UniRef:UniRef50_P47632", "UniRef:UniRef90_P78012" ], "nt": "ATGGCAAAGGAATTAGTATTTGGCAAGAATGCCCGCAATAAACTGCTAGCTGGTATTAATAAATTAGCAGACGCTGTTAAGGTAACCGTTGGTCCTAAAGGTCAAAACGTGATTTTAGGTCGGAAGTTTTCCAATCCTTTAATTACTAACGATGGAGTGACCATCGCTAAGGAAATTGAACTAACTGATCCTTTAGAAAACATTGGTGCTAAGGTTATTTCGGTAGCTGCTGTATCTACCAATGATATTGCTGGGGACGGTACTACCACTGCTACCATCTTGGCACAGGAAATGACTAACCGTGGTGTGGAAGCTGTTAATAATGGCGCTAATCCAGTTAATGTCCGCCGTGGGATTGAAGATGCTAGTCAGTTAATTATTACTGAGTTAGATAAGCGTTCCAAAAAGATTAATACCAACGAAGAAATCGAGCAAGTTGCAGCGATCTCTTCCGGTTCCAAGGAAATTGGGAAGCTGATTGCTCAAGCTATGGCCTTAGTTGGTAAAAACGGTGTCATTACCACCGATGATGCTAAGACGATTAACACTACCCTAGAAACCACTGAAGGGATTGAGTTTAAAGGTACTTACGCTTCCCCTTACATGGTTAGCGATCAAGAAAAAATGGAAGTGGTGTTAGACCAACCGAAGATCTTGGTCAGTGCGATGAAGATTAACACCATTAAGGAAATCTTACCGTTACTAGAAGGTAGCATGGAAAACGGTAACCCACTGTTAATCGTGGCACCTGACTTTGCTGAAGAAGTGGTAACTACTTTAGCGGTGAACAAGCTTCGCGGTACCATTAATGTAGTTGCTGTTAAATGTAACGAATACGGGGAACGCCAAAAGGCAGCTTTGGAAGATTTAGCCATTAGTACTGGCACCTTAGCTTACAACAATGAACTAGGTGGTGGCTTTAAGGATGTGACTGTAAACCACTTAGGGGAAGCTCGCCGTGTGCAAGTAGCCAAAGAAAAAACCACGGTTATTGGTGGTAAGGGTTCTAAGGAAACGATCCAAAAACACCTTGACCTTCTAAATGGTCGTTTAAAGCAAACTACCGAAAAGTACGATACCGATCTGTTAAAGGAACGGATTGCCCACCTCAGTCAAGGGGTAGCTGTAGTACGGGTTGGTGGTGCTACGGAACTAGCACAAAAAGAGTTAAAACTCCGCATTGAAGATGCCTTAAACTCGACTAAGGCAGCTGTTGAAGAAGGGATTATTTCCGGGGGTGGTATTGCTTTACTTAACGTATCCACTATCCTCAATGACAGTAAGTTGGCTGACAAGTACAAGGCAGAAACTAGTGCGGAAAACCTCAAGGAAATCCTGGTTGGTTATGAAATTGTGCGTAAGTCACTAGAAGCACCAGTGCGCCAAATTATTGAAAACTCCGGGGTTAACCCAGTTAAAGTATTTGCAGAACTACGCAGTGAAGCTGATGGTGTTGGTTTTGATGCTGAAACCAAAAAGAAGGTCGACATGATCCGCAGCGGTATTATTGACCCTACTAAGGTAACCAAAACTGCACTCGAAAAAGCTGCATCAGTAGCAAGTTCCTTAATTACCACCAGCGTTGCTGTGTATGATATTAAGGAAAACAAGGAAGGTAGCTTCCAAGAATAG", "aa": "MAKELVFGKNARNKLLAGINKLADAVKVTVGPKGQNVILGRKFSNPLITNDGVTIAKEIELTDPLENIGAKVISVAAVSTNDIAGDGTTTATILAQEMTNRGVEAVNNGANPVNVRRGIEDASQLIITELDKRSKKINTNEEIEQVAAISSGSKEIGKLIAQAMALVGKNGVITTDDAKTINTTLETTEGIEFKGTYASPYMVSDQEKMEVVLDQPKILVSAMKINTIKEILPLLEGSMENGNPLLIVAPDFAEEVVTTLAVNKLRGTINVVAVKCNEYGERQKAALEDLAISTGTLAYNNELGGGFKDVTVNHLGEARRVQVAKEKTTVIGGKGSKETIQKHLDLLNGRLKQTTEKYDTDLLKERIAHLSQGVAVVRVGGATELAQKELKLRIEDALNSTKAAVEEGIISGGGIALLNVSTILNDSKLADKYKAETSAENLKEILVGYEIVRKSLEAPVRQIIENSGVNPVKVFAELRSEADGVGFDAETKKKVDMIRSGIIDPTKVTKTALEKAASVASSLITTSVAVYDIKENKEGSFQE", "aa_hexdigest": "c64a2abdaf698cb2342265bde9053f1a", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001276EF", "ncbi_nrp_id": "WP_010874930.1", "uniref100_id": "UniRef100_P78012", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001276EF", "RefSeq:WP_010874930.1", "UniRef:UniRef100_P78012" ] }, "ips": { "uniref100_id": "UniRef100_P78012", "uniref90_id": "UniRef90_P78012" }, "psc": { "uniref90_id": "UniRef90_P78012", "gene": "groL", "product": "chaperonin GroEL", "ec_ids": [ "5.6.1.7" ], "uniref50_id": "UniRef50_P47632", "cog_id": "COG0459", "cog_category": "O", "go_ids": [ "GO:0005524", "GO:0005737", "GO:0016853", "GO:0042026", "GO:0051082", "GO:0140662" ] }, "pscc": { "uniref50_id": "UniRef50_P47632", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47632" ], "product": "Chaperonin GroEL" }, "genes": [ "groL" ], "id": "APKECPOBEN_1234", "locus": "GCA002090215_01234" }, { "type": "cds", "sequence": "contig_1", "start": 697368, "stop": 697718, "strand": "-", "frame": 2, "gene": "groES", "product": "co-chaperone GroES", "db_xrefs": [ "COG:COG0234", "COG:O", "GO:0005524", "GO:0005737", "GO:0044183", "KEGG:K04078", "RefSeq:WP_010874931.1", "SO:0001217", "UniParc:UPI0000127635", "UniRef:UniRef100_P75205", "UniRef:UniRef50_P47633", "UniRef:UniRef90_P75205" ], "nt": "ATGAAGATAACACCAATTCATGACAACATTTTGGTTTCACTAGTGGAATCAAACAAAGAAGAAGTGTCACAAAAAGGGATTATTACAGCACTAGCTAACCCTGATAAAAATGAAAGTGCCCACAAGGGAACTGTCGTTGCTTTAGGTGCAGGACCGGCATATGGTAAAAGCGAAAAACCGAAATATGCTTTTGGCATCGGTGACACGATCTACTTTAAGGAATACAGTGGTATTTCCTTTGAAGAGGAAGGTACCAAGTACAAGATTATTTCCTTGGAAGATGTCCTTGCTTTTGAAAAGCATGGTAATACAAAAACTACTACTGTAAAGAAAGGAGCTAAGAAAAAATAG", "aa": "MKITPIHDNILVSLVESNKEEVSQKGIITALANPDKNESAHKGTVVALGAGPAYGKSEKPKYAFGIGDTIYFKEYSGISFEEEGTKYKIISLEDVLAFEKHGNTKTTTVKKGAKKK", "aa_hexdigest": "ed0cbe8839984c80d949ab01ca30e622", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000127635", "ncbi_nrp_id": "WP_010874931.1", "uniref100_id": "UniRef100_P75205", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000127635", "RefSeq:WP_010874931.1", "UniRef:UniRef100_P75205" ] }, "ips": { "uniref100_id": "UniRef100_P75205", "uniref90_id": "UniRef90_P75205" }, "psc": { "uniref90_id": "UniRef90_P75205", "gene": "groES", "product": "co-chaperone GroES", "uniref50_id": "UniRef50_P47633", "cog_id": "COG0234", "cog_category": "O", "kegg_orthology_id": "K04078", "go_ids": [ "GO:0005737", "GO:0005524", "GO:0044183" ] }, "pscc": { "uniref50_id": "UniRef50_P47633", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47633" ], "product": "Co-chaperonin GroES" }, "genes": [ "groES" ], "id": "APKECPOBEN_1235", "locus": "GCA002090215_01235" }, { "type": "cds", "sequence": "contig_1", "start": 697906, "stop": 698292, "strand": "-", "frame": 1, "gene": null, "product": "putative protein MPN_575", "db_xrefs": [ "GO:0005886", "RefSeq:WP_010874932.1", "SO:0001217", "UniParc:UPI000013AD79", "UniRef:UniRef100_P75204", "UniRef:UniRef50_P75204", "UniRef:UniRef90_P75204" ], "nt": "ATGCTGCAAAATTTAGCTTTAAGCTTTCCTTTTATTACGCGTTTCTTCCAAAAGCAAATGCTTGGTTCCCAGAATTCTAGTGGTAAAACTCCTGGTTTCAATGAAGCCGAAGGAATAACGAGTAACATTTTCCAAATTGCTGGTGGAATTAGCCTTTTAGTTATTTTGTTGTTAATCATTGGTTTCTTGAGTTGTTTGTTGGGTGGCATCTTCCTACATAAGCACAAATACGCTGAAGTGGGTAGTCCCGCTCATGCAAAAACGAAAAATCTATTTGTTGCTTTCTTTGTTGTCGGTAGCTTATTGTTGTTAGTGGCCGTAGTCATGTTAATTGCTTTCGGTGTGCTTGATGCTAGTCTGCCACTTCCAAAAGAAAATAACTCTTAA", "aa": "MLQNLALSFPFITRFFQKQMLGSQNSSGKTPGFNEAEGITSNIFQIAGGISLLVILLLIIGFLSCLLGGIFLHKHKYAEVGSPAHAKTKNLFVAFFVVGSLLLLVAVVMLIAFGVLDASLPLPKENNS", "aa_hexdigest": "9d0d7d64a6c1f621bc8c2efc311497bc", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013AD79", "ncbi_nrp_id": "WP_010874932.1", "uniref100_id": "UniRef100_P75204", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013AD79", "RefSeq:WP_010874932.1", "UniRef:UniRef100_P75204" ] }, "ips": { "uniref100_id": "UniRef100_P75204", "uniref90_id": "UniRef90_P75204" }, "psc": { "uniref90_id": "UniRef90_P75204", "product": "Uncharacterized protein MPN_575", "uniref50_id": "UniRef50_P75204", "go_ids": [ "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75204", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75204" ], "product": "Uncharacterized protein MPN_575" }, "genes": [], "id": "APKECPOBEN_1236", "locus": "GCA002090215_01236" }, { "type": "cds", "sequence": "contig_1", "start": 698727, "stop": 698984, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00464.25" ], "nt": "TTGCAACAAGCCAACATTGTGTTGAACATGAACACGGTTCCTTTCGATAAGAATCCTGCCATAAATCCTTCCGGTATTCGTATTGGTACACCTGCCATGACTACTCGTGGCTTTAAGGAAAAGCACTTCCTTTATGTTGCTGCCTTAATTGACAAGATTATTAAATCTGACGGTAATAAGAAAGTTATTAAAGAAGTAAAAAAAGCGGTTTTAAAACTGTTAGAACGTTTTCCGTTGTATAAGGGATTGGAGTATTAG", "aa": "MQQANIVLNMNTVPFDKNPAINPSGIRIGTPAMTTRGFKEKHFLYVAALIDKIIKSDGNKKVIKEVKKAVLKLLERFPLYKGLEY", "aa_hexdigest": "1c7ac642cdc9098ecaa540745a491ff2", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00464.25", "name": "SHMT", "length": 49, "aa_cov": 0.5764705882352941, "hmm_cov": 0.9795918367346939, "evalue": 1.5729954581929956e-14, "score": 48.78683853149414, "start": 2, "stop": 50 } ], "seq_stats": { "molecular_weight": 9617.351999999995, "isoelectric_point": 9.939171409606931 }, "id": "APKECPOBEN_1237", "locus": "GCA002090215_01237" }, { "type": "cds", "sequence": "contig_1", "start": 698985, "stop": 699701, "strand": "-", "frame": 2, "gene": "glyA", "product": "Serine hydroxymethyltransferase", "db_xrefs": [ "COG:COG0112", "COG:E", "EC:2.1.2.1", "GO:0004372", "GO:0005829", "GO:0019264", "GO:0030170", "GO:0035999", "KEGG:K00600", "SO:0001217", "UniRef:UniRef50_P47634", "UniRef:UniRef90_P78011" ], "nt": "GTGCAACCGCACTCCGGTTCTTCTGCTAACTATGCCGTGTACTTAGCTCTACTTAAACCTGGCGATGCCATTTTAGGGCTTGACCTTAACTGTGGTGGGCACTTAACTCACGGTAATAAATTTAACTTTTCGGGTAAACAGTACCAGCCTTATTCCTACACCATCAACCCCGAAACCGAGATGTTGGACTATGATGAGGTGCTACGTGTTGCTCGGGAGGTAAAACCTAAACTAATTATCTGCGGTTTTTCCAATTACTCGAGAACCGTTGACTTTGAGCGCTTTAGTGCAATTGCGAAAGAAGTTGGTGCTTACCTTTTAGCGGACATTGCCCACATTGCGGGATTGGTAGCAGCTGGCTTGCACCCCAATCCCTTACCTTACACTGATGTGGTTACATCTACTACCCACAAAACACTGCGCGGTCCACGGGGAGGGTTAATTATGTCCAATAACGAAGCGATTATTCGCAAATTGGACAGCGGTGTCTTTCCGGGTTGTCAAGGTGGTCCCTTACAGCACGTAATTGCGGCTAAGTATGTTTGTTTTAAAGAAGCTTTACAACCAAAATACAAGCAGTACATCCAAAACGTTAAGACCAATGCAGCGAGCATGGCTAGTTGGTTCAAACAACAAGGTTACCGCGTCATTTCTAACGGAACTGATACCCACTTGTTCTCGCTTGATGTCGGTAAGGGTAAAGATGTTTCACAATGA", "aa": "MQPHSGSSANYAVYLALLKPGDAILGLDLNCGGHLTHGNKFNFSGKQYQPYSYTINPETEMLDYDEVLRVAREVKPKLIICGFSNYSRTVDFERFSAIAKEVGAYLLADIAHIAGLVAAGLHPNPLPYTDVVTSTTHKTLRGPRGGLIMSNNEAIIRKLDSGVFPGCQGGPLQHVIAAKYVCFKEALQPKYKQYIQNVKTNAASMASWFKQQGYRVISNGTDTHLFSLDVGKGKDVSQ", "aa_hexdigest": "658de7ed1af763f065344de750164f70", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI000FEF2304", "query_cov": 0.9705882352941176, "subject_cov": 0.9352226720647774, "identity": 0.623, "score": 285.0, "evalue": 1.95e-94, "valid": false, "product": "serine hydroxymethyltransferase", "ec_ids": [ "2.1.2.1" ], "uniref50_id": "UniRef50_A0A661GFR2", "kegg_orthology_id": "K00600" }, "genes": [ "glyA" ], "truncated": "both", "pseudogene": { "start": 698727, "stop": 699947, "observations": { "mutation": [ 698987, 699710 ], "stop": [ 698987, 699710 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P78011", "query_cov": 1.0, "subject_cov": 0.5862068965517241, "identity": 0.996, "score": 486.0, "evalue": 2.75e-171, "gene_start": 1, "gene_end": 238, "reference_start": 83, "reference_end": 320, "reference_sequence": "MEPKIRRILNKELQRQRDCICLIASENYVSRDILEVTGSILTNKYAEGYPTRRFYEGCEVVDESESLAINTCKELFGAKWANVQPHSGSSANYAVYLALLKPGDAILGLDLNCGGHLTHGNKFNFSGKQYQPYSYTINPETEMLDYDEVLRVAREVKPKLIICGFSNYSRTVDFERFSAIAKEVGAYLLADIAHIAGLVAAGLHPNPLPYTDVVTSTTHKTLRGPRGGLIMSNNEAIIRKLDSGVFPGCQGGPLQHVIAAKYVCFKEALQPKYKQYIQNVKTNAASMASWFKQQGYRVISNGTDTHLFSLDVGKGKDVSQWLQQANIVLNMNTVPFDKNPAINPSGIRIGTPAMTTRGFKEKHFLYVAALIDKIIKSDGNKKVIKEVKKAVLKLLERFPLYKGLEY" }, "paralog": false, "identity": 0.9950738916256158, "score": 811.0, "evalue": 8.93e-302, "description": "Internal stop codon at 698987, 699710. Nonsense mutation around 698987, 699710.", "psc": { "uniref90_id": "UniRef90_P78011", "gene": "glyA", "product": "Serine hydroxymethyltransferase", "ec_ids": [ "2.1.2.1" ], "uniref50_id": "UniRef50_P47634", "cog_id": "COG0112", "cog_category": "E", "kegg_orthology_id": "K00600", "go_ids": [ "GO:0005829", "GO:0004372", "GO:0030170", "GO:0019264", "GO:0035999" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P78011", "EC:2.1.2.1", "UniRef:UniRef50_P47634", "COG:COG0112", "COG:E", "KEGG:K00600", "GO:0005829", "GO:0004372", "GO:0030170", "GO:0019264", "GO:0035999" ] }, "pscc": { "uniref50_id": "UniRef50_P47634", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47634" ], "product": "Serine hydroxymethyltransferase" } }, "id": "APKECPOBEN_1238", "locus": "GCA002090215_01238" }, { "type": "cds", "sequence": "contig_1", "start": 699708, "stop": 699947, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00464.25" ], "nt": "ATGGAACCAAAGATTAGAAGAATCCTCAATAAGGAATTGCAAAGACAAAGAGATTGCATTTGTTTAATAGCATCAGAGAACTATGTGAGTAGGGACATCCTAGAGGTCACTGGTTCTATTTTGACCAATAAGTACGCTGAGGGTTATCCTACTAGACGCTTTTATGAAGGTTGTGAGGTAGTTGATGAATCAGAGAGCTTAGCCATTAATACCTGTAAGGAATTATTTGGTGCGAAGTGA", "aa": "MEPKIRRILNKELQRQRDCICLIASENYVSRDILEVTGSILTNKYAEGYPTRRFYEGCEVVDESESLAINTCKELFGAK", "aa_hexdigest": "99b359e63f0562419aa7e22af2aef923", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A1F2Y6L7", "query_cov": 0.9367088607594937, "subject_cov": 0.8505747126436781, "identity": 0.5539999999999999, "score": 83.6, "evalue": 1.76e-19, "valid": false, "product": "Serine hydroxymethyltransferase-like domain-containing protein", "ec_ids": [ "2.1.2.1" ], "uniref50_id": "UniRef50_A0A838MK70", "kegg_orthology_id": "K00600" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00464.25", "name": "SHMT", "length": 77, "aa_cov": 0.9746835443037974, "hmm_cov": 1.0, "evalue": 1.776958759300126e-29, "score": 98.00299072265625, "start": 2, "stop": 78 } ], "seq_stats": { "molecular_weight": 9119.335399999994, "isoelectric_point": 5.453383064270019 }, "id": "APKECPOBEN_1239", "locus": "GCA002090215_01239" }, { "type": "cds", "sequence": "contig_1", "start": 700025, "stop": 700162, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGTGTAGTGTTGTTTGGCAAATTGGGTATAGTAATGGGTAGTATTGCGGTTGCCAAAGATAAGATCATAACCAATGCTGCTCTAGTTCGGATTGAGGGAATTGCCCTCACCCTGAAAGCGCAGCATTTGGACTAA", "aa": "MGVVLFGKLGIVMGSIAVAKDKIITNAALVRIEGIALTLKAQHLD", "aa_hexdigest": "e9ff952172233911abf3e8ed993ef43e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4689.6711, "isoelectric_point": 9.52289905548096 }, "id": "APKECPOBEN_1240", "locus": "GCA002090215_01240" }, { "type": "cds", "sequence": "contig_1", "start": 700109, "stop": 700312, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01732.22" ], "nt": "ATGTATGGTCATGGTTTAGGAATCGATCAGGTTAACCTAAGAAAAGGGACTTCTAGTTCTTTAGTGATTAACCAAAAGCGTCAAATTGTGGGAATCTATTTTGCTACGGTAATTACTAATCCCAAGAAGGCTGTTCGTAACGATGTTGGTTTAGTCCAAATGCTGCGCTTTCAGGGTGAGGGCAATTCCCTCAATCCGAACTAG", "aa": "MYGHGLGIDQVNLRKGTSSSLVINQKRQIVGIYFATVITNPKKAVRNDVGLVQMLRFQGEGNSLNPN", "aa_hexdigest": "8973777533218428a702c0160bd05934", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01732.22", "name": "Mycop_pep_DUF31", "length": 34, "aa_cov": 0.5074626865671642, "hmm_cov": 1.0, "evalue": 3.287095108046899e-08, "score": 28.994007110595703, "start": 2, "stop": 35 } ], "seq_stats": { "molecular_weight": 7357.3937000000005, "isoelectric_point": 10.277243614196774 }, "id": "APKECPOBEN_1241", "locus": "GCA002090215_01241" }, { "type": "cds", "sequence": "contig_1", "start": 700373, "stop": 701014, "strand": "-", "frame": 3, "gene": null, "product": "putative protein MPN_577", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75203", "UniRef:UniRef90_P75203" ], "nt": "TTGAGCAATCCGAAGGACTATGCACCGTTTAATAAAGCTAGCATTGGCAACAGTTTAACTACGGTATTTTGTCTAGGGAAATATATTAACCCTCAATTGTTTAAGCTACGTACTGATGTAAGCAACGCCTTTGTGTCAATACAAACTTCCACTATTCCCAAAACAGCCTTTGTAGCACGCGACTTTGTGCCCTTACAAAACCGCGGTAACCAGTGGGTAGCACCAGTTCGTGCATCAGAAGATGATCCTGCACTAGCAAAAAGCTATTTGGATTTTGCGATCATAGAAGTTCCACTCTTTTTACACAACCAAATGGATAAACAGATCTATGACCACTTTATGCGCCCCGCCATCAATACCTATGAACGGTTAGGTAACAGTGTGGGAATCTTTGCTTACCAACCTATGGCAAGCTTTAAGCGAGATAGCTATTTTGCTTTAGGTTATCCCCAAGTAGAGAGCAATATTGCTGCACTTAACTTGAACCAAACTGAAGTTAAACCTACTCGCCCAGAGGATGTTGCTCAAGTTACTTTTAAAGAGCCTTGGTCAGTAGATCACCACCGCGAAATTCCTACTTTGACTACCAATCAACTTACCACCATTAAAACAAAACACTTTTCTGGTAGTAAGTTATCTTGA", "aa": "MSNPKDYAPFNKASIGNSLTTVFCLGKYINPQLFKLRTDVSNAFVSIQTSTIPKTAFVARDFVPLQNRGNQWVAPVRASEDDPALAKSYLDFAIIEVPLFLHNQMDKQIYDHFMRPAINTYERLGNSVGIFAYQPMASFKRDSYFALGYPQVESNIAALNLNQTEVKPTRPEDVAQVTFKEPWSVDHHREIPTLTTNQLTTIKTKHFSGSKLS", "aa_hexdigest": "0ad465293935d5aa7e54199c4b8c9619", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 700109, "stop": 701269, "observations": { "mutation": [ 700375, 701095, 701107, 701128, 701227 ], "stop": [ 700375, 701095, 701107, 701128, 701227 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75203", "query_cov": 1.0, "subject_cov": 0.5518134715025906, "identity": 0.995, "score": 429.0, "evalue": 2.31e-149, "gene_start": 1, "gene_end": 213, "reference_start": 86, "reference_end": 298, "reference_sequence": "MKTVTAAGGVKRKEWKTNYLINTIPKKSEALHDLYDLSFSMQLANNKWRVFGTGWLIDWKKPKRTHNLSEPFYLYLATNLHIAVALSNPKDYAPFNKASIGNSLTTVFCLGKYINPQLFKLRTDVSNAFVSIQTSTIPKTAFVARDFVPLQNRGNQWVAPVRASEDDPALAKSYLDFAIIEVPLFLHNQMDKQIYDHFMRPAINTYERLGNSVGIFAYQPMASFKRDSYFALGYPQVESNIAALNLNQTEVKPTRPEDVAQVTFKEPWSVDHHREIPTLTTNQLTTIKTKHFSGSKLSWPFDHTKSFKIKNKWLGQNYQMYGHGLGIDQVNLRKGTSSSLVINQKRQIVGIYFATVITNPKKAVRNDVGLVQMLRFQGEGNSLNPN" }, "paralog": false, "identity": 0.9844559585492227, "score": 759.0, "evalue": 2.86e-282, "description": "Internal stop codon at 700375, 701095, 701107, 701128, 701227. Nonsense mutation around 700375, 701095, 701107, 701128, 701227.", "psc": { "uniref90_id": "UniRef90_P75203", "product": "Uncharacterized protein MPN_577", "uniref50_id": "UniRef50_P75203", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75203", "UniRef:UniRef50_P75203" ] }, "pscc": { "uniref50_id": "UniRef50_P75203", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75203" ], "product": "Uncharacterized protein MPN_577" } }, "id": "APKECPOBEN_1242", "locus": "GCA002090215_01242" }, { "type": "cds", "sequence": "contig_1", "start": 702376, "stop": 702798, "strand": "-", "frame": 1, "gene": null, "product": "putative protein MPN_580", "db_xrefs": [ "GO:0003723", "GO:0003724", "GO:0005524", "RefSeq:WP_010874937.1", "SO:0001217", "UniParc:UPI000013AD84", "UniRef:UniRef100_P75200", "UniRef:UniRef50_P75200", "UniRef:UniRef90_P75200" ], "nt": "ATGACCGCCCAGTATTACCAACTAGACGAAAAGTTTAATCAGCAAACCTATGAACAGTATGGTAAAGGCTTAGCCTTAAATGATTTGTATATCCGCAGGGGCGCTTCTGGTAGTTTAGTCTTCAATCAGGATCAACAAATATCAGGCATCTTTTTTGCAGTAGCTAGTCATTCCGACCCTAAACCAGGTGAAAAAACTCTGGTACAACTGTTACGCTTGCCAGTTGATGAAAGTACTACAGCGACGGTAAAGGACAACTGTGTGCCCTATGATTTAATCTTTGGCAACCGCAATACTACCCATTACTATACCCAATTTGCCAAGCAACACCACACCCATTTGTATGAGCAAATTCAACAAAGTAATGACCAGTTAATTAAGTTTATTGATCGCAAAAATGTGTCCTGTTCAATGGTTTCGTAA", "aa": "MTAQYYQLDEKFNQQTYEQYGKGLALNDLYIRRGASGSLVFNQDQQISGIFFAVASHSDPKPGEKTLVQLLRLPVDESTTATVKDNCVPYDLIFGNRNTTHYYTQFAKQHHTHLYEQIQQSNDQLIKFIDRKNVSCSMVS", "aa_hexdigest": "f8138e022066dd8b567e1f4d8128bd17", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013AD84", "ncbi_nrp_id": "WP_010874937.1", "uniref100_id": "UniRef100_P75200", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013AD84", "RefSeq:WP_010874937.1", "UniRef:UniRef100_P75200" ] }, "ips": { "uniref100_id": "UniRef100_P75200", "uniref90_id": "UniRef90_P75200" }, "psc": { "uniref90_id": "UniRef90_P75200", "product": "Uncharacterized protein MPN_580", "uniref50_id": "UniRef50_P75200", "go_ids": [ "GO:0005524", "GO:0003723", "GO:0003724" ] }, "pscc": { "uniref50_id": "UniRef50_P75200", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75200" ], "product": "Uncharacterized protein MPN_580" }, "genes": [], "id": "APKECPOBEN_1243", "locus": "GCA002090215_01243" }, { "type": "cds", "sequence": "contig_1", "start": 702881, "stop": 703267, "strand": "-", "frame": 3, "gene": null, "product": "putative protein MPN_581", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75199", "UniRef:UniRef90_P75199" ], "nt": "ATGGCTTATGCAGCGGTGGACTTTGTTGATTATCGGTTTGACAATAAACATTGGAAATCCTTAAATGAAATTTGTCCGGAAGTTGGTTTAAAACCTTATGCTGATTTTGCCGTGTTAGAAGTACCCTTGTACTTAAACAACAAGTTGGATTACCAAGTATGGGAAAACTTTATCAAACCCGCCATTGCTACCTATAAGGCCTTAGGTGATAGTACAAACTTGTTTGCAAGTACGTCATCTTCTGATTTACAAAATGACACCTATTTTGCTCTTGGTTATCCCTTACTAGAGAGTAACATTGATGCGATTCACTTTAACCAAACAGGTGCCACTTTAACTAGTGTTCCGGTTAAAGACCGCATTAAAGTTAAAAACCAAAGCTTCTGA", "aa": "MAYAAVDFVDYRFDNKHWKSLNEICPEVGLKPYADFAVLEVPLYLNNKLDYQVWENFIKPAIATYKALGDSTNLFASTSSSDLQNDTYFALGYPLLESNIDAIHFNQTGATLTSVPVKDRIKVKNQSF", "aa_hexdigest": "7af1985afa574e988a4eafa9b8f1ad0b", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 702866, "stop": 703567, "observations": { "mutation": [ 702883, 703501, 703534 ], "stop": [ 702883, 703501, 703534 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75199", "query_cov": 1.0, "subject_cov": 0.4830188679245283, "identity": 1.0, "score": 264.0, "evalue": 1.47e-87, "gene_start": 1, "gene_end": 128, "reference_start": 133, "reference_end": 260, "reference_sequence": "MQLEKVPRAEYQTKWATNTVPKPTDFYHQLYNLSFSLTLTSQRWNTYGTGWLIDWKDTSTNENKFTAYLATNFHVADTLKNPHDYPPYNQVDNTQDLTTSFRIGKYTDPSLFGLYTNVKHAFVDVQLAALPKMAYAAVDFVDYRFDNKHWKSLNEICPEVGLKPYADFAVLEVPLYLNNKLDYQVWENFIKPAIATYKALGDSTNLFASTSSSDLQNDTYFALGYPLLESNIDAIHFNQTGATLTSVPVKDRIKVKNQSFWTLNK" }, "paralog": false, "identity": 0.9871244635193133, "score": 468.0, "evalue": 2.35e-170, "description": "Internal stop codon at 702883, 703501, 703534. Nonsense mutation around 702883, 703501, 703534.", "psc": { "uniref90_id": "UniRef90_P75199", "product": "Uncharacterized protein MPN_581", "uniref50_id": "UniRef50_P75199", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75199", "UniRef:UniRef50_P75199" ] }, "pscc": { "uniref50_id": "UniRef50_P75199", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75199" ], "product": "Uncharacterized protein MPN_581" } }, "id": "APKECPOBEN_1244", "locus": "GCA002090215_01244" }, { "type": "cds", "sequence": "contig_1", "start": 703754, "stop": 703861, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCCAGTTTGCAAAAAAATAACACTCACTTTTATGGGCAAATTAAATCTGCGCGAGACCCTAGTTTAAAATATGTAGATAACGAATCAAAGGTAAAAAATACTTAA", "aa": "MPSLQKNNTHFYGQIKSARDPSLKYVDNESKVKNT", "aa_hexdigest": "0639fd0271805c332aaa09bb3493acd1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4039.487000000001, "isoelectric_point": 9.601679420471193 }, "id": "APKECPOBEN_1245", "locus": "GCA002090215_01245" }, { "type": "cds", "sequence": "contig_1", "start": 703944, "stop": 704099, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01732.22" ], "nt": "ATGTATGGCAAGGGAATTGGGATCACTAATGGTAGTTTAAGTCGTGGCGCTTCGGGTTCCTTAGTCTTAAATAACAAGCGACAGATTGTGGCCATTTACTTTGCTAGTAGAATCACTGAAACACAAGAATGGGGTCTAGCGCAGCTGTTACGCTGA", "aa": "MYGKGIGITNGSLSRGASGSLVLNNKRQIVAIYFASRITETQEWGLAQLLR", "aa_hexdigest": "2591b3d16ac1c27cebacf4a224baedf9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01732.22", "name": "Mycop_pep_DUF31", "length": 34, "aa_cov": 0.6666666666666666, "hmm_cov": 1.0, "evalue": 1.556864402122609e-10, "score": 36.63825225830078, "start": 2, "stop": 35 } ], "seq_stats": { "molecular_weight": 5540.316699999999, "isoelectric_point": 10.419847679138183 }, "id": "APKECPOBEN_1246", "locus": "GCA002090215_01246" }, { "type": "cds", "sequence": "contig_1", "start": 704562, "stop": 705116, "strand": "-", "frame": 2, "gene": null, "product": "putative lipoprotein MPN_582", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P75198", "UniRef:UniRef90_P75198" ], "nt": "TTGGCACTAACTGTTTTAAGTGCTTGTTCAGCCTTTCGAAAACCTGATAAATTCGAAGGGTATTACATTAACAATATTCCTGGTAGTGCAGAAATCGATCATGCCAACTATGATTTAACCTTTTTTTTAGGTTTTTTTAACAGAATTGAAAAAACCGAAAAAGATAAAAGTCAAATAGATTTTGGTTCCATTCACGGAACGGGTTGGTTAATTGACTGGAAGGAAGTTGACAAGAAAGATAAAAAAGCTAATAAATTTACTGTTTACTTAGCTACTAATTTACACGTCATACAGGCCTTAAAAAACCGAGAAGATCATCCGCCTTACAACCAATTTGATATCAATTTTGTGCGAACCGTTGATTTTAGAATTGGCAAATACACTGATGTTAAACAATTTGTCGCACCAAGCAAATTAGGCTTACCCAACTCTACCCAAGCTTTTGTAGCAGCACAACCAACGGTACTTCCTAAAACTGCTTTCATTGCACATGACTTTGTGAACTACACTTTATCCAAAGATCAAAAGAATAAAAAACAAAGAGAACAGCAATGA", "aa": "MALTVLSACSAFRKPDKFEGYYINNIPGSAEIDHANYDLTFFLGFFNRIEKTEKDKSQIDFGSIHGTGWLIDWKEVDKKDKKANKFTVYLATNLHVIQALKNREDHPPYNQFDINFVRTVDFRIGKYTDVKQFVAPSKLGLPNSTQAFVAAQPTVLPKTAFIAHDFVNYTLSKDQKNKKQREQQ", "aa_hexdigest": "3c5d5de4050202365fc2f3f442bb9315", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 704259, "stop": 705158, "observations": { "mutation": [ 704564, 705155 ], "stop": [ 704564, 705155 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75198", "query_cov": 1.0, "subject_cov": 0.4043956043956044, "identity": 0.9890000000000001, "score": 374.0, "evalue": 3.71e-127, "gene_start": 1, "gene_end": 184, "reference_start": 15, "reference_end": 198, "reference_sequence": "MWVALKRFCFLSGLLALTVLSACSAFRKPDKFEGYYINNIPGSAEIDHANYDLTFSLGFFNRIEKTEKDKSQIDFGSIHGTGWLIDWKEVDKKDKKANKFTVYLATNLHVIQALKNREDHPPYNQFDINFVRTVDFRIGKYTDVKQFVAPSKLGLPNSTQAFVAAQPTVLPKTAFIAHDFVNYTLSKDQKNKKQREQQWKVQIKPNKDEVHPYADFAVLELPLFLNNSSDRQIFDHFIQPAIRAYKQLGDSLNIFAYPTLDQFKHSHYYVLGYPYIAKKLPTLFVNQTGKEKTVPGETAQIPNDQPFVSTINEEGPHLGRIKSDKFNGGTWAWNHDNTKNFPFNRQFRGKEYQMYGKGIGITNGSLSRGASGSLVLNNKRQIVAIYFASRITETQEWGLAQLLRWKPRSVLNEEKDSVAYDLIFGNSNTKKYYAQFAKKITLTFMGKLNLRETLV" }, "paralog": false, "identity": 0.9832775919732442, "score": 595.0, "evalue": 1.22e-216, "description": "Internal stop codon at 704564, 705155. Nonsense mutation around 704564, 705155.", "psc": { "uniref90_id": "UniRef90_P75198", "product": "Uncharacterized lipoprotein MPN_582", "uniref50_id": "UniRef50_P75198", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75198", "UniRef:UniRef50_P75198", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75198", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75198" ], "product": "Uncharacterized lipoprotein MPN_582" } }, "id": "APKECPOBEN_1247", "locus": "GCA002090215_01247" }, { "type": "cds", "sequence": "contig_1", "start": 705233, "stop": 705376, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGCCTATGACTTAATCTTTGGTAACAAAGACACTAAAAAGTACTACGCTCAATTTGCTAAACAACATAAAACCCATTTGTTTGAGCAAATTAAGCAAAGCAACGACCAACAATTTACCTTTGTTAAAAACCAAAAGTCTTAA", "aa": "MAYDLIFGNKDTKKYYAQFAKQHKTHLFEQIKQSNDQQFTFVKNQKS", "aa_hexdigest": "093ceb9e08a6c6cb4bb8c2259fa78da6", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5687.358000000002, "isoelectric_point": 9.574667167663574 }, "id": "APKECPOBEN_1248", "locus": "GCA002090215_01248" }, { "type": "cds", "sequence": "contig_1", "start": 705629, "stop": 705991, "strand": "-", "frame": 3, "gene": null, "product": "putative protein MPN_583", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75197", "UniRef:UniRef90_P75197" ], "nt": "GTGTTAGAGCTAACCTTATTTTTAGATAACCCAGTTGATAAAAAGGTGTTTGACCATTTTATCCAACCAGCCATCAAAACTTATGAGCAATTAGGTAATAGCTTAAAGCTGTTTGCCAAGCCAACATTAACTGAACTCAAAAACCACCGCTATTATTTATCGGGTTATCCCTTCTTGGAAAACAAGGTTAATGTATTGAGTGTTAATCAAATTAAGAAAGATACATCTACAGAAACAACAATAAATGGACAAACAGTACAACAAAGTAACCACGAACAACCCCTTATTGTGCGATCGACAATAGCAAAGCCAACCCTAATTAGAAATCAAGGTGATGACTTTAATGGCAGTAACTGGGTTTGA", "aa": "MLELTLFLDNPVDKKVFDHFIQPAIKTYEQLGNSLKLFAKPTLTELKNHRYYLSGYPFLENKVNVLSVNQIKKDTSTETTINGQTVQQSNHEQPLIVRSTIAKPTLIRNQGDDFNGSNWV", "aa_hexdigest": "790d2211dd4e7a156dd27d13919386a5", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 705596, "stop": 706273, "observations": { "mutation": [ 705631, 706075 ], "stop": [ 705631, 706075 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75197", "query_cov": 1.0, "subject_cov": 0.5333333333333333, "identity": 0.992, "score": 241.0, "evalue": 3.8e-79, "gene_start": 1, "gene_end": 120, "reference_start": 95, "reference_end": 214, "reference_sequence": "MPNNAQAFVNVQTTVIPKTAFTAHDFVNYTLPKDKKKQNDTENKKKQPKDGENDKQKEQAETQPFEWIQQKDADDKKESNTANTSDSLAYADFAVLELTLFLDNPVDKKVFDHFIQPAIKTYEQLGNSLKLFAKPTLTELKNHRYYLSGYPFLENKVNVLSVNQIKKDTSTETTINGQTVQQSNHEQPLIVRSTIAKPTLIRNQGDDFNGSNWVWNYDNSRVFRF" }, "paralog": false, "identity": 0.9866666666666667, "score": 448.0, "evalue": 2.35e-163, "description": "Internal stop codon at 705631, 706075. Nonsense mutation around 705631, 706075.", "psc": { "uniref90_id": "UniRef90_P75197", "product": "Uncharacterized protein MPN_583", "uniref50_id": "UniRef50_P75197", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75197", "UniRef:UniRef50_P75197" ] }, "pscc": { "uniref50_id": "UniRef50_P75197", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75197" ], "product": "Uncharacterized protein MPN_583" } }, "id": "APKECPOBEN_1249", "locus": "GCA002090215_01249" }, { "type": "cds", "sequence": "contig_1", "start": 706073, "stop": 706273, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCCAATAATGCTCAAGCCTTTGTTAATGTCCAAACCACAGTAATTCCCAAAACCGCTTTTACAGCGCATGACTTTGTCAACTACACTTTGCCAAAAGACAAAAAGAAGCAAAATGATACAGAAAATAAAAAGAAGCAACCAAAGGATGGAGAAAACGATAAGCAAAAGGAGCAAGCGGAAACCCAACCGTTTGAGTGA", "aa": "MPNNAQAFVNVQTTVIPKTAFTAHDFVNYTLPKDKKKQNDTENKKKQPKDGENDKQKEQAETQPFE", "aa_hexdigest": "27f00c9c5972bf2d144a27d58588c4bf", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7593.327200000004, "isoelectric_point": 7.967276954650878 }, "id": "APKECPOBEN_1250", "locus": "GCA002090215_01250" }, { "type": "cds", "sequence": "contig_1", "start": 706468, "stop": 706713, "strand": "-", "frame": 1, "gene": null, "product": "DUF31 family putative serine protease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00143DB747", "UniRef:UniRef90_UPI00143DB747" ], "nt": "ATGATTGTGGCTTTCAAGCGCCTTGGTTTTCTCGCTAGCATAGCTTCCTTAACTGTATTAAGTGCTTGTGCTGCAGTGCCACTAGAAAAGTGGGATCAGTTCTATGTAAGTAATATTCCTAGTAGTGGTTCGATTCACCGTGCCAACTATGATTTAACCTTTGCTTTAACTTTTACTAATAAAACTACTAACAATGGGCAAGATAACTTTAGCGCCTTTTCTGGCACTGGCTGGCTCATTGATTGA", "aa": "MIVAFKRLGFLASIASLTVLSACAAVPLEKWDQFYVSNIPSSGSIHRANYDLTFALTFTNKTTNNGQDNFSAFSGTGWLID", "aa_hexdigest": "27a4ae5b6919c157144c26f8a16e0504", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00143DB747", "query_cov": 0.9629629629629629, "subject_cov": 0.896551724137931, "identity": 1.0, "score": 158.0, "evalue": 5.08e-49, "valid": true, "product": "DUF31 family putative serine protease", "uniref50_id": "UniRef50_UPI00143DB747" }, "pscc": { "uniref50_id": "UniRef50_UPI00143DB747", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00143DB747" ], "product": "DUF31 family putative serine protease" }, "genes": [], "id": "APKECPOBEN_1251", "locus": "GCA002090215_01251" }, { "type": "cds", "sequence": "contig_1", "start": 706777, "stop": 706932, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGCTTATGATTTAATCTTTGGTAATAAAAATACCACAAAATACTATGCTCAGTTTGTCAAAGAACACAAAACCCATTTATACGAGCAAATTAAGCAAATTAACAATGATGAATTCCGCTTTGTCGAAAAGACTAAAAGGCATAACTTGGGGTAA", "aa": "MAYDLIFGNKNTTKYYAQFVKEHKTHLYEQIKQINNDEFRFVEKTKRHNLG", "aa_hexdigest": "51af47c8df0916edb372643869b2f736", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6251.007699999999, "isoelectric_point": 9.347158241271973 }, "id": "APKECPOBEN_1252", "locus": "GCA002090215_01252" }, { "type": "cds", "sequence": "contig_1", "start": 706969, "stop": 707184, "strand": "-", "frame": 1, "gene": null, "product": "putative protein MPN_584", "db_xrefs": [ "GO:0003723", "GO:0003724", "GO:0005524", "SO:0001217", "UniRef:UniRef50_P75196", "UniRef:UniRef90_P75196" ], "nt": "TTGAACTATGATCTCTCCCAGAAATTTAAGGTTTATCTTGAGTTTTCGGGCAAAAAGTACCGAATCTATGGCCAAAGTATAGGCATTAGTCATCTTGATTTAGGCGGTGGATCTTCTGGTTCTTTGTTGTTAAATGATAAGAAACAAATCGCTGGCATTTACTTTGGCGTTGATGGTGCTAACAATGAATTAGGCTTAGCACAGTTATTACGCTGA", "aa": "MNYDLSQKFKVYLEFSGKKYRIYGQSIGISHLDLGGGSSGSLLLNDKKQIAGIYFGVDGANNELGLAQLLR", "aa_hexdigest": "1aa1fda7670c3abf5a06f733d472adf5", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 706777, "stop": 707184, "observations": { "mutation": [ 706971 ], "stop": [ 706971 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75196", "query_cov": 1.0, "subject_cov": 0.5259259259259259, "identity": 1.0, "score": 141.0, "evalue": 5.23e-42, "gene_start": 1, "gene_end": 71, "reference_start": 1, "reference_end": 71, "reference_sequence": "MNYDLSQKFKVYLEFSGKKYRIYGQSIGISHLDLGGGSSGSLLLNDKKQIAGIYFGVDGANNELGLAQLLRWKPETYHNDEARSVAYDLIFGNKNTTKYYAQFVKEHKTHLYEQIKQINNDEFRFVEKTKRHNLG" }, "paralog": false, "identity": 0.9851851851851852, "score": 272.0, "evalue": 5.57e-96, "description": "Internal stop codon at 706971. Nonsense mutation around 706971.", "psc": { "uniref90_id": "UniRef90_P75196", "product": "Uncharacterized protein MPN_584", "uniref50_id": "UniRef50_P75196", "go_ids": [ "GO:0005524", "GO:0003723", "GO:0003724" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75196", "UniRef:UniRef50_P75196", "GO:0005524", "GO:0003723", "GO:0003724" ] }, "pscc": { "uniref50_id": "UniRef50_P75196", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75196" ], "product": "Uncharacterized protein MPN_584" } }, "id": "APKECPOBEN_1253", "locus": "GCA002090215_01253" }, { "type": "cds", "sequence": "contig_1", "start": 707270, "stop": 707689, "strand": "-", "frame": 3, "gene": null, "product": "putative lipoprotein MPN_585", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P75195", "UniRef:UniRef90_P75195" ], "nt": "GTGCAAACAAGTGTAATTCCCAAAACCGCTTTTGCTGCTAGGGACTTTGTGGATTACAGTTTTCCTCAAGAACAAAAAGATAAAGAAAAGAGAAAACAACAATGGGTGAAAAACAGTCATACTAAGAATTCTGATGTTCAGCCTTTTGCTGAGTTTGCAATTTTAGAAATTCCCTTATTTTCGAAAAGTAAAGTAGATCGCAAAATCTTTAATCACTTTATTCAACCAGCCATACGCACTTATAAACAGTTAGGTGATAGCCTTAACATTTTTGCTAACCCCACACTGGATCAATTAAAACAAAATCGCTATTATGTGTTAGGTTATCCTTTTTTAAAAAATAAAGTGTCATCACTATTTCTTAACCAAACGGGAAAAAAGAAAGGAATATTTAGGCGAAAATACACAAATATCCCATGA", "aa": "MQTSVIPKTAFAARDFVDYSFPQEQKDKEKRKQQWVKNSHTKNSDVQPFAEFAILEIPLFSKSKVDRKIFNHFIQPAIRTYKQLGDSLNIFANPTLDQLKQNRYYVLGYPFLKNKVSSLFLNQTGKKKGIFRRKYTNIP", "aa_hexdigest": "db4ef5465f033749355a44ef45576c3d", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 707246, "stop": 707989, "observations": { "mutation": [ 707272, 707788, 707917, 707929 ], "stop": [ 707272, 707788, 707917, 707929 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75195", "query_cov": 1.0, "subject_cov": 0.4602649006622517, "identity": 0.993, "score": 276.0, "evalue": 1.5e-91, "gene_start": 1, "gene_end": 139, "reference_start": 156, "reference_end": 294, "reference_sequence": "MLVVFKRLGFIVSIFSLTFLSACAAFDKWQEFYINNIPSSTEIHRFNYDLNFSLTFTNRITSNGEAKFSVTYGTGWLIDWKEPDKKKENDPFRAYLATNLHVAASLINPQDYEPYKNKDDAGWTTTFRLGKYTKVSDFVSPNQFGLPNAAQALVNVQTSVIPKTAFAARDFVDYSFPQEQKDKEKRKQQWVKNSHTKNSDVQPFAEFAILEIPLFSKSKVDRKIFNHFIQPAIRTYKQLGDSLNIFANPTLDQLKQNRYYVLGYPFLKNKVSSLFLNQTGKKKGIFRRKYTNIPWKTSYYLN" }, "paralog": false, "identity": 0.9838056680161943, "score": 488.0, "evalue": 2.37e-177, "description": "Internal stop codon at 707272, 707788, 707917, 707929. Nonsense mutation around 707272, 707788, 707917, 707929.", "psc": { "uniref90_id": "UniRef90_P75195", "product": "Uncharacterized lipoprotein MPN_585", "uniref50_id": "UniRef50_P75195", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75195", "UniRef:UniRef50_P75195", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75195", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75195" ], "product": "Uncharacterized lipoprotein MPN_585" } }, "id": "APKECPOBEN_1254", "locus": "GCA002090215_01254" }, { "type": "cds", "sequence": "contig_1", "start": 707927, "stop": 708154, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGGTTGTTTTCAAGCGTCTTGGTTTTATTGTTAGCATATTCTCTTTAACTTTTTTAAGTGCTTGTGCAGCCTTTGATAAGTGGCAAGAGTTTTACATTAACAATATCCCTAGTAGTACGGAAATCCACCGATTCAACTATGACTTAAACTTCTCGCTTACTTTTACTAATAGAATCACTAGCAACGGTGAAGCCAAATTTAGTGTCACATATGGTACTGGTTGA", "aa": "MLVVFKRLGFIVSIFSLTFLSACAAFDKWQEFYINNIPSSTEIHRFNYDLNFSLTFTNRITSNGEAKFSVTYGTG", "aa_hexdigest": "a220d5ac26ea612ca60edbad7584f6ed", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8588.689499999993, "isoelectric_point": 7.907579231262207 }, "id": "APKECPOBEN_1255", "locus": "GCA002090215_01255" }, { "type": "cds", "sequence": "contig_1", "start": 708624, "stop": 709178, "strand": "-", "frame": 2, "gene": null, "product": "DUF31 family putative serine protease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75197", "UniRef:UniRef90_UPI0006BA639D" ], "nt": "TTGTGTCACCAAACGAAAATAGGATTACCTAATGGTGCACAAGCCTTTGTATCAGCGCAGACAAGCACAATTCCCAAAACGGCTTTCACTGCGCACGATTTTGTGGATTACACCTTGCCCCAAGAACAAAAAGGTAAAGAACAAAAGGAGCAGCAGTGGATGAAGAACAGTCAAACTAAGGATACTGATGTTCATCCCTATGCTGATTTTGCAGTCTTAGAAATCCTTTTGTTTCCTGACAGTTCTACTGATCGCAAAATTTTTAATCACTTTATCCAACCTGCCATTAGAGCCTATAAACAATTAGGTGATAGCTTAAATATCTTTGCTAATCAAACCCTAGATCAACCTAAACATAACCGTTATTACTTGTTGGGTTATCCTTTTTTAAGAAATAAAGCGGCATCATTGTTTTTAAATCAAACGGAACAAAGAAAGGAACATATGGACAAAACTACCCAAATACTTCATGAAAAACCATTTGTTGTTTTAACCGATCCAGCTAAGCCTACTTTAATTAGGAATAAAGGCGCTAATTTTACTGGCAACACTTGA", "aa": "MCHQTKIGLPNGAQAFVSAQTSTIPKTAFTAHDFVDYTLPQEQKGKEQKEQQWMKNSQTKDTDVHPYADFAVLEILLFPDSSTDRKIFNHFIQPAIRAYKQLGDSLNIFANQTLDQPKHNRYYLLGYPFLRNKAASLFLNQTEQRKEHMDKTTQILHEKPFVVLTDPAKPTLIRNKGANFTGNT", "aa_hexdigest": "b2a88f5047c3f6a8bfe14e03f198a73b", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0006BA639D", "query_cov": 1.0, "subject_cov": 0.9108910891089109, "identity": 1.0, "score": 374.0, "evalue": 6.52e-131, "valid": true, "product": "DUF31 family putative serine protease", "uniref50_id": "UniRef50_P75197" }, "pscc": { "uniref50_id": "UniRef50_P75197", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75197" ], "product": "Uncharacterized protein MPN_583" }, "genes": [], "id": "APKECPOBEN_1256", "locus": "GCA002090215_01256" }, { "type": "cds", "sequence": "contig_1", "start": 709345, "stop": 709599, "strand": "-", "frame": 1, "gene": null, "product": "putative lipoprotein MPN_587", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P75193", "UniRef:UniRef90_P75193" ], "nt": "ATGGTAATTGCCTTAAAGCGGTTTAGTTTTCTAGCTAGCATAGCTACATTAACTGTGCTCAACGCTTGTGCAACAATTTCACTATCTGATAAATCTGGTGAGTTTTACATTAACAATATTCCTAGTAGTGCTGAATTACACCGGATTAACTATGACTTGACCTTCTCTTTAGGTTTTACCAACAGAATTCAGACTAAAAAGAAGAATGAAGTAAACTTCGTTGCCTTTTATGGTACTGGTTTGTTGATTGACTGA", "aa": "MVIALKRFSFLASIATLTVLNACATISLSDKSGEFYINNIPSSAELHRINYDLTFSLGFTNRIQTKKKNEVNFVAFYGTGLLID", "aa_hexdigest": "f7d61b1db985e8fab48937b4f1ff3aae", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 709147, "stop": 709599, "observations": { "mutation": [ 709224, 709347 ], "stop": [ 709224, 709347 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75193", "query_cov": 1.0, "subject_cov": 0.56, "identity": 1.0, "score": 161.0, "evalue": 1.73e-49, "gene_start": 1, "gene_end": 84, "reference_start": 1, "reference_end": 84, "reference_sequence": "MVIALKRFSFLASIATLTVLNACATISLSDKSGEFYINNIPSSAELHRINYDLTFSLGFTNRIQTKKKNEVNFVAFYGTGLLIDWKEGNQYQPFRVYLATNVHVAAGLKNEADYLPYSQPDAELGWTVDFRLGKYTNVKDFVSPNENRIT" }, "paralog": false, "identity": 0.9866666666666667, "score": 293.0, "evalue": 9.2e-104, "description": "Internal stop codon at 709224, 709347. Nonsense mutation around 709224, 709347.", "psc": { "uniref90_id": "UniRef90_P75193", "product": "Uncharacterized lipoprotein MPN_587", "uniref50_id": "UniRef50_P75193", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75193", "UniRef:UniRef50_P75193", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75193", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75193" ], "product": "Uncharacterized lipoprotein MPN_587" } }, "id": "APKECPOBEN_1257", "locus": "GCA002090215_01257" }, { "type": "cds", "sequence": "contig_1", "start": 709657, "stop": 710103, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01732.22" ], "nt": "GTGATCCAAGACAAAGGTGTGGTGAACTTTGCTTCCTTTAGCAACTTTACCTTGAAGTACCACGAAAAGCGGTTCCAACAATACGGCTATGGCTTAATGTTGAATGACACTAATTTTCCGGGTGGCTCTTCGGGTAGTCCTTTAATTGGTAAAGACAATAAATTGAACAGCATTTACTTTGGGGTGTTGGAAATTTACCAATCTGGTAGTCTTGCACGCAATGACATTGGGATGAGCCAAATCTTACGTACTCCACAGAACGATAAAGGTTCAAGTATTTCTAAGGGTAGCTATGACTTGATCTTTGGCGATAAGAACACCAAGAATTATTACGCTAAGTTTGCTAAAGATCATCAAACTCATTTGTATCAGAAGATTAAAGAAAGCAAAGACGAACAATTCCGCTTTGTGGAAACACAAGAGACAACTAATAATTTAGGCAATTAA", "aa": "MIQDKGVVNFASFSNFTLKYHEKRFQQYGYGLMLNDTNFPGGSSGSPLIGKDNKLNSIYFGVLEIYQSGSLARNDIGMSQILRTPQNDKGSSISKGSYDLIFGDKNTKNYYAKFAKDHQTHLYQKIKESKDEQFRFVETQETTNNLGN", "aa_hexdigest": "f5d75ab01227c9950491b5ffc0dc0555", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01732.22", "name": "Mycop_pep_DUF31", "length": 51, "aa_cov": 0.34459459459459457, "hmm_cov": 0.8823529411764706, "evalue": 5.387867654321662e-15, "score": 51.307559967041016, "start": 11, "stop": 61 } ], "seq_stats": { "molecular_weight": 16819.534199999987, "isoelectric_point": 8.981106758117676 }, "id": "APKECPOBEN_1258", "locus": "GCA002090215_01258" }, { "type": "cds", "sequence": "contig_1", "start": 710239, "stop": 711252, "strand": "-", "frame": 1, "gene": null, "product": "putative lipoprotein MPN_588", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_Q50339", "UniRef:UniRef90_Q50339" ], "nt": "ATGAGACTGCAATTCAAGTTATTGGGTTTCTTAACACTGCTGGGTACCAGCACTATTCTTAGTGCCTGTGCTGCAACCCAGCCAAACTTTGAACCCAATAACATTGAAGAAAGTGGTCCCATTACACCTACCACACCTACAACTGATGTACCTAAACCAACCGCAGAGGTAGTGCCTGTTAACCGTGGCTTTCACTTCCAAACCAATAAGGTACCTAGTGAAAGTGATGTTTTTAAGCATAACTATGATCTAACTTTTTCGTTGAACTTTACCAATAAGTCTAACGATATCTATGGCACCGGCTGGTTATTTGACTGGAAGGGTGATGAAAAGGCATTAGGTATTGATGGCAGTTTTGTCCCTAGCATTACAAGCAACATTGATAACTCCCTTCTAAAGGATGATCAATTCACCGTTTATTTAGCCACTAACTTACACGTAGCTGATGCTTTAAGGAATGATCAGGATTACGAACCCTACAAAAAAGATCAGAACAAGCAAGATTTTACTGAGAATACTAAAACTGAATTCTTTTCTTTGGGTAAATACTTAGAAGGTGAGCAGTTAAAGCAGTACATTTCTAAAGAAGAAAATCAATCAGCCAATCAAACTGATAAAGCCTTGGTTTCGATTCAGGCCTCCAATATTCCCAAAACGGCTTACACTGCTACTGACTTTGTTGATATGAATAGTTACAGTTATAACAACATCACAACAAGTCTTCCTGGCAACTATGCTGACTTTGCCGTGATTGAAGTTAACCTAAACCTTAAAAATCAACGCGATCAGCAAATTCTTCATGACTTTGTCAAACCAGCAATTAAGGCTTACAAGGCTTTAGGTGATACTTTGGAACTGTTTAGTGCCAAACCACTCAACCAGTTCATCGAACAAAACTACTACTTGTTAGGTTATCCAGTGATTAATAAAGGAAACAATACCGCTAACCTTTTATTAGCGCAACAAAAGAGCTTTGATCACAACAATTCGGATCATAACCAAAAGAGCCAGTGA", "aa": "MRLQFKLLGFLTLLGTSTILSACAATQPNFEPNNIEESGPITPTTPTTDVPKPTAEVVPVNRGFHFQTNKVPSESDVFKHNYDLTFSLNFTNKSNDIYGTGWLFDWKGDEKALGIDGSFVPSITSNIDNSLLKDDQFTVYLATNLHVADALRNDQDYEPYKKDQNKQDFTENTKTEFFSLGKYLEGEQLKQYISKEENQSANQTDKALVSIQASNIPKTAYTATDFVDMNSYSYNNITTSLPGNYADFAVIEVNLNLKNQRDQQILHDFVKPAIKAYKALGDTLELFSAKPLNQFIEQNYYLLGYPVINKGNNTANLLLAQQKSFDHNNSDHNQKSQ", "aa_hexdigest": "cdd50434066eedfcc3904d9d1939c39a", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 709936, "stop": 711252, "observations": { "mutation": [ 710241 ], "stop": [ 710241 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q50339", "query_cov": 1.002967359050445, "subject_cov": 0.6353383458646616, "identity": 0.997, "score": 654.0, "evalue": 8.66e-234, "gene_start": 1, "gene_end": 337, "reference_start": 1, "reference_end": 338, "reference_sequence": "MRLQFKLLGFLTLLGTSTILSACAATQPNFEPNNIEESGPITPTTPTTDVPKPTAEVVPVNRGFHFQTNNKVPSESDVFKHNYDLTFSLNFTNKSNDIYGTGWLFDWKGDEKALGIDGSFVPSITSNIDNSLLKDDQFTVYLATNLHVADALRNDQDYEPYKKDQNKQDFTENTKTEFFSLGKYLEGEQLKQYISKEENQSANQTDKALVSIQASNIPKTAYTATDFVDMNSYSYNNITTSLPGNYADFAVIEVNLNLKNQRDQQILHDFVKPAIKAYKALGDTLELFSAKPLNQFIEQNYYLLGYPVINKGNNTANLLLAQQKSFDHNNSDHNQKSQWFTKDQSYINKLDRIPVLTNNYRAYNESTGSQLFANQQNESWLNGVVIQDKGVVNFASFSNFTLKYHEKRFQQYGYGLMLNDTNFPGGSSGSPLIGKDNKLNSIYFGVLEIYQSGSLARNDIGMSQILRTPQNDKGSSISKGSYDLIFGDKNTKNYYAKFAKDHQTHLYQKIKESKDEQFRFVETQETTNNLGN" }, "paralog": false, "identity": 0.9954441913439636, "score": 882.0, "evalue": 0.0, "description": "Internal stop codon at 710241. Nonsense mutation around 710241.", "psc": { "uniref90_id": "UniRef90_Q50339", "product": "Uncharacterized lipoprotein MPN_588", "uniref50_id": "UniRef50_Q50339", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q50339", "UniRef:UniRef50_Q50339", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_Q50339", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50339" ], "product": "Uncharacterized lipoprotein MPN_588" } }, "id": "APKECPOBEN_1259", "locus": "GCA002090215_01259" }, { "type": "cds", "sequence": "contig_1", "start": 711468, "stop": 711845, "strand": "-", "frame": 2, "gene": null, "product": "putative protein MPN_589", "db_xrefs": [ "GO:0003723", "GO:0003724", "GO:0005524", "SO:0001217", "UniRef:UniRef50_Q50338", "UniRef:UniRef90_Q50338" ], "nt": "ATGCCAACACTTACCAAAAACTTAAGGCAAGGTCAACCAGTTGCTGGTAGTAAAGTGCACACAGATTACAGTAACGGCTTCCTTAATGAATATTCCTTAAATCAAGGAATTGTTGATTTTGGTACTTTTAAAAGTGCTATCACTGATTATCATGGATATGAATATAAAAACCACGGCTATGGCTTAGCTCTAACTGATACTGATTTACTAGGTGGATCGAGTGGCAGTTTAGTGTTTAACCAAGACAAAAAGATTAGCAGTATTTATTCAGCTGCTACTGAATCAGATAGTGTTGGCTATGCCCAGCTATTGCCTGTTCCTAAAGATGTTAATGGGGTAAGTTTAGTTAAATACAGTTACGACCTTATACTTTGGTGA", "aa": "MPTLTKNLRQGQPVAGSKVHTDYSNGFLNEYSLNQGIVDFGTFKSAITDYHGYEYKNHGYGLALTDTDLLGGSSGSLVFNQDKKISSIYSAATESDSVGYAQLLPVPKDVNGVSLVKYSYDLILW", "aa_hexdigest": "9e1a1b21ff5e9fd437a4d89b7f92c259", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 711468, "stop": 711938, "observations": { "mutation": [ 711893 ], "stop": [ 711893 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q50338", "query_cov": 1.0, "subject_cov": 0.7961783439490446, "identity": 1.0, "score": 254.0, "evalue": 2.24e-85, "gene_start": 1, "gene_end": 125, "reference_start": 32, "reference_end": 156, "reference_sequence": "MGYPTLWTDDAKLFEWTKTEQRFNHDDFYGSMPTLTKNLRQGQPVAGSKVHTDYSNGFLNEYSLNQGIVDFGTFKSAITDYHGYEYKNHGYGLALTDTDLLGGSSGSLVFNQDKKISSIYSAATESDSVGYAQLLPVPKDVNGVSLVKYSYDLILWW" }, "paralog": false, "identity": 0.9871794871794872, "score": 319.0, "evalue": 3.41e-113, "description": "Internal stop codon at 711893. Nonsense mutation around 711893.", "psc": { "uniref90_id": "UniRef90_Q50338", "product": "Uncharacterized protein MPN_589", "uniref50_id": "UniRef50_Q50338", "go_ids": [ "GO:0005524", "GO:0003723", "GO:0003724" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q50338", "UniRef:UniRef50_Q50338", "GO:0005524", "GO:0003723", "GO:0003724" ] }, "pscc": { "uniref50_id": "UniRef50_Q50338", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50338" ], "product": "Uncharacterized protein MPN_589" } }, "id": "APKECPOBEN_1260", "locus": "GCA002090215_01260" }, { "type": "cds", "sequence": "contig_1", "start": 712079, "stop": 712327, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAATGATTATGAGTTTTTCAATCAATTTGATTACTTTGACGATCCAACTGAATCTTTCACTTTAGGTAAGTTCGTTGATGGTAATGAAATAAAGCAAATAGTTCCTGATGCTATGCATGAACCTAGCTTAGTGCGGATTGAAACTTCAAAATTGCCAAAAACTGCTTACACGACAACACTTTTTATTAATGATTTGGGGGAATATAGATTACCTGCCGCTGACTTTGCTGTATTGGAATCAATCTAG", "aa": "MNDYEFFNQFDYFDDPTESFTLGKFVDGNEIKQIVPDAMHEPSLVRIETSKLPKTAYTTTLFINDLGEYRLPAADFAVLESI", "aa_hexdigest": "7feff5d78befa06371addbd56dcc2d15", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9432.427499999996, "isoelectric_point": 4.195479393005371 }, "id": "APKECPOBEN_1261", "locus": "GCA002090215_01261" }, { "type": "cds", "sequence": "contig_1", "start": 712403, "stop": 712624, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCCTGTAAACAACAAAAGTGTTAGTGCAGTTCAACCAGAAGACAAGCAAGCATTAAAACCAGACCCTGCTTTAGGTGAATACACCTCTGACTTAAAGTTCAATACTAACTTCATTCCAAAAAAGGATGATCCCTTTCGTCCCTTTTATGATCTAAGTTTTACGCTCCAGTTTCAAGATCCATATACAGCTACTTACGGTACTGGTTGGCTCATTGATTGA", "aa": "MPVNNKSVSAVQPEDKQALKPDPALGEYTSDLKFNTNFIPKKDDPFRPFYDLSFTLQFQDPYTATYGTGWLID", "aa_hexdigest": "e8bd5c455fb50bb570a386a0bfaa2cff", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8334.204199999996, "isoelectric_point": 4.611427116394043 }, "id": "APKECPOBEN_1262", "locus": "GCA002090215_01262" }, { "type": "cds", "sequence": "contig_1", "start": 712889, "stop": 713950, "strand": "-", "frame": 3, "gene": null, "product": "putative protein MPN_591", "db_xrefs": [ "GO:0003723", "GO:0003724", "GO:0005524", "RefSeq:WP_010874948.1", "SO:0001217", "UniParc:UPI000013ADAA", "UniRef:UniRef100_Q50336", "UniRef:UniRef50_Q50336", "UniRef:UniRef90_Q50336" ], "nt": "GTGGCTAACAGCTTAAGGAACGTTAATGATTACGATCCTTTTAAATACCGCCCCAGTTATTTTGATCTAGAGGCACCAACCGAATCATTTGCTTTAGGTAAGTTTGTCGATGCGATTGAAGTTAAGCAGATGGCACTAGATGCCTTTAGTGAATCTAGCATGGTCAGTATTGCAACTTCACAGCTACCCAAAACAGCTTACACAGCTACAAGTTTTATTGAAAGCGAGTATTATAGTTTTCCTTATTTATTTAACGACCAAAAATATTACTGGGATTATTTTGATTACAAAATCCCTGCCGCTGACTTTGCCGTATTAGAGTTAGAACTGGATTTAAACAACCAGCAGGATCAGCAAATTAAAGATCATTTCATCGATCCAGCGATCAAGGCTTATAAACAATTAGGTGATAGTACCGGTTTGTTTGCCACCAAACCATTAACTGAATATCAAAACGATACCCATTACCTCTTGGGCTATCCGGTTGTACCTACTGATCATACACAACTGTGGGAATGCAAACAAGGGGCAGAGCGCTTTAGCTATGGTTATTTTTACAGTAATATGGCTAGACTAACCAAAAACCTAAGGCAGGGCGATCCAAACGCTGGTAGTAAGACACACATCGAATACAGCAATGAGTTACTCGATAAGGACTCAATGGATCAAGGTATTGTGCGTTTTAGTACCTTTTTGGGTGCTAACATTAATTACCACGATTACGATTACCGTCAACAGGGTTACGGTTTAACCTTAACCGACACTAACTTGCCAGGTGGATCGAGTGGCAGTTTAGTGTTTAACCAAGACAAAAAGATTAGCAGTATTTATTCAGCTGCTACTGAATCAGATAGTGTTGGCTATGCTCAGTTATTACGAACTCCTAGGGATGTCAACGGTATAAGTGTAGTTTCACAAAGCTATGACCTCATCTTTGGTGATAGCAACACTAAGCGTTACTATGCGATGTTTGCTAAAAAACAGCAAACTCACTTGTACAGTGAAATCCTTAAAAGTACTGATGAACAGTACCGTTATGTGGTAGACAAACAATTCAATTAA", "aa": "MANSLRNVNDYDPFKYRPSYFDLEAPTESFALGKFVDAIEVKQMALDAFSESSMVSIATSQLPKTAYTATSFIESEYYSFPYLFNDQKYYWDYFDYKIPAADFAVLELELDLNNQQDQQIKDHFIDPAIKAYKQLGDSTGLFATKPLTEYQNDTHYLLGYPVVPTDHTQLWECKQGAERFSYGYFYSNMARLTKNLRQGDPNAGSKTHIEYSNELLDKDSMDQGIVRFSTFLGANINYHDYDYRQQGYGLTLTDTNLPGGSSGSLVFNQDKKISSIYSAATESDSVGYAQLLRTPRDVNGISVVSQSYDLIFGDSNTKRYYAMFAKKQQTHLYSEILKSTDEQYRYVVDKQFN", "aa_hexdigest": "e61db966f3ccde618ac15ccaaa1edfdf", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013ADAA", "ncbi_nrp_id": "WP_010874948.1", "uniref100_id": "UniRef100_Q50336", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013ADAA", "RefSeq:WP_010874948.1", "UniRef:UniRef100_Q50336" ] }, "ips": { "uniref100_id": "UniRef100_Q50336", "uniref90_id": "UniRef90_Q50336" }, "psc": { "uniref90_id": "UniRef90_Q50336", "product": "Uncharacterized protein MPN_591", "uniref50_id": "UniRef50_Q50336", "go_ids": [ "GO:0005524", "GO:0003723", "GO:0003724" ] }, "pscc": { "uniref50_id": "UniRef50_Q50336", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50336" ], "product": "Uncharacterized protein MPN_591" }, "genes": [], "id": "APKECPOBEN_1263", "locus": "GCA002090215_01263" }, { "type": "cds", "sequence": "contig_1", "start": 714473, "stop": 714928, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01732.22" ], "nt": "TTGGTACAAAATCAAATTAACTATTTACCCTTTGCACAAAATGACCCTAAAGGCGTAATGGACTTTAGCAAGTATTTAAATTACGTTTTTAACTATCACGAAAAGCAATACCAACACCATGGTTATGGTTTATTGTTAGAAGACACTGACTTCCCGGGTGGTTCCAGTGGTAGTCCGTTATTTAACCAAAACAAACAGATTAACAGTATTTACTTTGCGGCTTTACCAAGTAAAAGTTACGGTGTATCGCAAATTCTCCGCGCTACGCAAAACAAAGACAAGTCCAAAAACTACGATTTAATCTTTGGTGATAGCAACACCAAGAAATACTATGCTCAATTTGCTAAGGAGCACAAAACCCACTTGTACCATCAAATTCTTCAAAGTAACGATGAACAGTTCCGCTTTGTCGAAAACGATCAAACTGTAACAAGTCAAACACCATTTAAAAGCTAA", "aa": "MVQNQINYLPFAQNDPKGVMDFSKYLNYVFNYHEKQYQHHGYGLLLEDTDFPGGSSGSPLFNQNKQINSIYFAALPSKSYGVSQILRATQNKDKSKNYDLIFGDSNTKKYYAQFAKEHKTHLYHQILQSNDEQFRFVENDQTVTSQTPFKS", "aa_hexdigest": "12695e155d9926e4f36d3ccd5db99013", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01732.22", "name": "Mycop_pep_DUF31", "length": 71, "aa_cov": 0.47019867549668876, "hmm_cov": 0.9154929577464789, "evalue": 1.6851917329565098e-16, "score": 56.25593948364258, "start": 3, "stop": 73 } ], "seq_stats": { "molecular_weight": 17569.239399999984, "isoelectric_point": 7.887013816833496 }, "id": "APKECPOBEN_1264", "locus": "GCA002090215_01264" }, { "type": "cds", "sequence": "contig_1", "start": 715037, "stop": 715480, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01732.22" ], "nt": "TTGGGTAAATACATTGATGCCCAAACTGTGCAAAAGTTAACTCCAGAGTACCAAAATCTTAAGCACCTCAATAACCGGAATAGTGATGCATTAGTTTCAATTCAAACATCGAAGTTACCAAAAACTGCTTACACTGCCACTGACTTTATCAAAACTGCTCAGTACAAATACAATCACATAGTGAGTAACACAGTTTATGAGTTGGACTTATTCCAAAATGCCGTAAGTTACGCTGACTTTGCGGTGTTGGAATTAGAGTTAAATTTAGCCAACAATCGTGACCAGCAAATCTTTGACAGTTTTATAAATCCAGCGGTAACTGCTTACGAAAAGCTAGGTAACAGTTTGGGTCTCTTTTCCAACCTGCAATTAGATCAGTATGTTGATGATACCCATTATCTATTGGGTTATCCGTTACTTAAACGTGAAAAGACGTCTTACTGA", "aa": "MGKYIDAQTVQKLTPEYQNLKHLNNRNSDALVSIQTSKLPKTAYTATDFIKTAQYKYNHIVSNTVYELDLFQNAVSYADFAVLELELNLANNRDQQIFDSFINPAVTAYEKLGNSLGLFSNLQLDQYVDDTHYLLGYPLLKREKTSY", "aa_hexdigest": "196463e8abfcd48ad4f870f9190e8a0e", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01732.22", "name": "Mycop_pep_DUF31", "length": 152, "aa_cov": 0.9659863945578231, "hmm_cov": 0.9473684210526315, "evalue": 9.077707146819816e-31, "score": 103.17800903320312, "start": 2, "stop": 143 } ], "seq_stats": { "molecular_weight": 16871.721399999995, "isoelectric_point": 5.521191978454589 }, "id": "APKECPOBEN_1265", "locus": "GCA002090215_01265" }, { "type": "cds", "sequence": "contig_1", "start": 715670, "stop": 716038, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGGTTTAAACTCAAGGGTTTTGGTTTTCTCACACTGTTCGCCAGTCAAGCATTTCTAACTGCTTGCTCTGCTACGCTAACAGTAGCTAACACTAATCACAAAAATGAAAGTGATAAGTTTGTGATTTTTGAACCTCAACCACCATTGAGCCAAACTATCCCCAAACCAGAGGCTGAACCAGTTATAGAGCCCGATGCTGTTGCTACACCACCTGTGCAGAATGCCGAGGTGCAAATTAAGCCTGACAGCTCCAAGGGTGTTTACAGTCCTGGTTTTAAGTTCAACACTAACTTTATTCCTAAAGTAAATACTAAGTATCGGCCGGGCTATGATCTTAGCTTTGCCTTAAAGTTTGGTACTAGTTGA", "aa": "MGFKLKGFGFLTLFASQAFLTACSATLTVANTNHKNESDKFVIFEPQPPLSQTIPKPEAEPVIEPDAVATPPVQNAEVQIKPDSSKGVYSPGFKFNTNFIPKVNTKYRPGYDLSFALKFGTS", "aa_hexdigest": "b9906f6d0939d177fcf7279089c58415", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13317.044699999979, "isoelectric_point": 8.709501457214355 }, "id": "APKECPOBEN_1266", "locus": "GCA002090215_01266" }, { "type": "cds", "sequence": "contig_1", "start": 716139, "stop": 716507, "strand": "-", "frame": 2, "gene": null, "product": "putative protein MPN_593", "db_xrefs": [ "GO:0005886", "RefSeq:WP_010874950.1", "SO:0001217", "UniParc:UPI000013ADB0", "UniRef:UniRef100_Q50334", "UniRef:UniRef50_Q50334", "UniRef:UniRef90_Q50334" ], "nt": "ATGAACAAAAAAGAAAGCACTACCACTAAAAAGCAGTGGTTTAAAAAGTGTTCTTTTAAGAAGTTAAAAGCGGAAATTTGCAACATGTTGCCAACAACGCCGCACAACACCAAGCGCACCTTAATCTGGGTGATTGTTTTTAGCTTTATTACTTTTCTTAGTTTTATTTTTGCTTATGTTTGCTTTAACTATGCTCCAGTATCCACCGGTTTCTTGTACTTCTTAGGAGCAGTGTTTTTACTAATTGGCTTTGCCTTTGCTATCTTGAGTTTTGTAGCAATGGTTAAATTTGTGGCTGATTATTTTGCCAATCGTTTTAGTAACACTCAGCTCAAAATGGACTGTGATTGTGCTAAAACGAAAAAGTAA", "aa": "MNKKESTTTKKQWFKKCSFKKLKAEICNMLPTTPHNTKRTLIWVIVFSFITFLSFIFAYVCFNYAPVSTGFLYFLGAVFLLIGFAFAILSFVAMVKFVADYFANRFSNTQLKMDCDCAKTKK", "aa_hexdigest": "e886a167d041086ca03c2e9933835d60", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013ADB0", "ncbi_nrp_id": "WP_010874950.1", "uniref100_id": "UniRef100_Q50334", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013ADB0", "RefSeq:WP_010874950.1", "UniRef:UniRef100_Q50334" ] }, "ips": { "uniref100_id": "UniRef100_Q50334", "uniref90_id": "UniRef90_Q50334" }, "psc": { "uniref90_id": "UniRef90_Q50334", "product": "Uncharacterized protein MPN_593", "uniref50_id": "UniRef50_Q50334", "go_ids": [ "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_Q50334", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50334" ], "product": "Uncharacterized protein MPN_593" }, "genes": [], "id": "APKECPOBEN_1267", "locus": "GCA002090215_01267" }, { "type": "cds", "sequence": "contig_1", "start": 716733, "stop": 717035, "strand": "-", "frame": 2, "gene": null, "product": "putative protein MPN_594", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniParc:UPI0001DD643B", "UniRef:UniRef100_P75191", "UniRef:UniRef50_P75191", "UniRef:UniRef90_P75191" ], "nt": "ATGAGAGGGAAAAATAGGAATAAATGCAACAGCATTATTTTTTTAACAGTTGGTTTACTGTTGTTTATAGCAGCTTTAGCGCTTGGTGTATTAGTACTTTTTAACGGGTATCAGGTTAATGTCAATGCTAATGGAGTTGACTTGAAACCCTTTGAGATTGTGCATTTTCCATTTGCGGTCAAAACTTTTCTTTCTGTTTTAACCTTTGTGTTAGCAGCCTTTGGTTTTGTGTGTATGGTAGCGAGTTTTCTCTACTTTGTCAGTTTTAAGAAGCTTAAACCAAAGGCTAATTCTGCTAGTTAG", "aa": "MRGKNRNKCNSIIFLTVGLLLFIAALALGVLVLFNGYQVNVNANGVDLKPFEIVHFPFAVKTFLSVLTFVLAAFGFVCMVASFLYFVSFKKLKPKANSAS", "aa_hexdigest": "652f9e3e0df54c69fe055bbf982575e8", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD643B", "uniref100_id": "UniRef100_P75191", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD643B", "UniRef:UniRef100_P75191" ] }, "ips": { "uniref100_id": "UniRef100_P75191", "uniref90_id": "UniRef90_P75191" }, "psc": { "uniref90_id": "UniRef90_P75191", "product": "Uncharacterized protein MPN_594", "uniref50_id": "UniRef50_P75191", "go_ids": [ "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75191", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75191" ], "product": "Uncharacterized protein MPN_594" }, "genes": [], "id": "APKECPOBEN_1268", "locus": "GCA002090215_01268" }, { "type": "cds", "sequence": "contig_1", "start": 717019, "stop": 717477, "strand": "-", "frame": 1, "gene": "rpiB", "product": "ribose 5-phosphate isomerase B", "db_xrefs": [ "COG:COG0698", "COG:G", "EC:5.3.1.6", "GO:0004751", "GO:0009052", "GO:0019316", "KEGG:K01808", "RefSeq:WP_010874952.1", "SO:0001217", "UniParc:UPI000013973D", "UniRef:UniRef100_P53527", "UniRef:UniRef50_P47636", "UniRef:UniRef90_P53527" ], "nt": "ATGCAAATGAACCATCCAATTTACATTGCTTCTGACCACACTGGTTTAGAGCTCAAAAGCTTAGTGATAAAACACTTAGAACAACAAAAGTTGCAAGTGATTGATTTAGGTCCTACAGAGCTAGATCCACTTGATGACTACCCGGACTATGCCTTTTTGTTAGCGCAAACAATGCAAGCTAATCCTAATAGTTTAGGCATCCTTATTTGTGGTACTGGTGTGGGTGTTTGTATGGCCGCTAACAAGGCTAAGGGTATCTTAGCTGCTTTAGTGGTAGACAGTAAAACAGCAGCATTGGCACGCCAACATGATGACGCCAATGTGCTGTGTTTGTCTAGCCGCTTTGTGGTTCCTGAAGAAAACATTAAAATAGTAGATGAATTTCTCCAGGCGCAGTTTGAGGGTGGGCGCCATAGTAAACGTGTCGGTAAGATCATTGCTTATGAGAGGGAAAAATAG", "aa": "MQMNHPIYIASDHTGLELKSLVIKHLEQQKLQVIDLGPTELDPLDDYPDYAFLLAQTMQANPNSLGILICGTGVGVCMAANKAKGILAALVVDSKTAALARQHDDANVLCLSSRFVVPEENIKIVDEFLQAQFEGGRHSKRVGKIIAYEREK", "aa_hexdigest": "6b3e3d8771c22daa47888aaef1965a1e", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013973D", "ncbi_nrp_id": "WP_010874952.1", "uniref100_id": "UniRef100_P53527", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013973D", "RefSeq:WP_010874952.1", "UniRef:UniRef100_P53527" ] }, "ips": { "uniref100_id": "UniRef100_P53527", "uniref90_id": "UniRef90_P53527" }, "psc": { "uniref90_id": "UniRef90_P53527", "gene": "rpiB", "product": "ribose 5-phosphate isomerase B", "ec_ids": [ "5.3.1.6" ], "uniref50_id": "UniRef50_P47636", "cog_id": "COG0698", "cog_category": "G", "kegg_orthology_id": "K01808", "go_ids": [ "GO:0004751", "GO:0009052", "GO:0019316" ] }, "pscc": { "uniref50_id": "UniRef50_P47636", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47636" ], "product": "Probable ribose-5-phosphate isomerase B" }, "genes": [ "rpiB" ], "id": "APKECPOBEN_1269", "locus": "GCA002090215_01269" }, { "type": "cds", "sequence": "contig_1", "start": 717477, "stop": 719186, "strand": "-", "frame": 2, "gene": null, "product": "putative MG397-like protein", "db_xrefs": [ "GO:0016020", "RefSeq:WP_010874953.1", "SO:0001217", "UniParc:UPI0000139743", "UniRef:UniRef100_Q50333", "UniRef:UniRef50_P47637", "UniRef:UniRef90_Q50333" ], "nt": "ATGGTTGTCATTGCTGCTTTATTGGGATCGTTAGCTGTCTTAGCTTTTCTGTTTTACTTGTGGTATTTAACGATTTTCATTATTGTGCATAAAAACCAGCAATACTTGGAACAGACCAAGGCGCTGTACAACAAGTTAAAACTCAGTAATTTCAACTCGGTAATTGTGCCGTTTCAACTGTTGCGCAGTGAAAAGAAAGCGCTGGAACAACCAGTGAAATTGCTCAAAAGTTTTCAAGAGAGCTTTAACAGCGAAATCAATTCCGCTTTAGAACAGCTGTTAGTTTTTAGTGAACCAAAAGCGCTTTACAACTGCTTTTGGTTTAACCGTAATATTAAGTTGTTGCGCAAAAACTTACAAGGATTACATGACAAGCAACAGCGTTATGTTAAGCTGACTAAAAACGCAATTGCGTACTTTGACAGTAGTTATGATACCCTAGTATTCTACCGACAAGCCTTTTGTTTACTAGTTAGTTTTATTAATGACTTTTTGGTGCATAAGTACGATTCCTTGTTTTACCTCAACATTATTAACCGCATTTCCTTATTGTTTAAGGATGTGGAACTACACATCAAAAATAAGGATGTGAAACAGCAAAACCAAGCGTTGAATAAACTCCACAATAACTTAGCACAAACCATTATTACCGCATCAAGACAATACTTCTTTGATGTCAAAGTAGGCTATTTGAACTACCACTTCCAAATTTTGAGTCAAAAGGTAACGCAATTGCGCTCGATGAAAAACAAAGCAATTAACTCCCAAGAGGTAGCTAAGTTTCAGGAATTAATTACTAATATTGGTGCTACTTTGGCGGAATGTAGCCAAGCGTTGGGCAACATTAACTTAGCGTTGTGTGAACAGCGCATTAACCAAGCTAAGGAACAGATTGATGTGTTATCTAATGCTGTTAGTGTTAAAGAAAAAGCGATTAGCTTAGTGGTATCTAATGTGGATAGCTTCTTAAAGTCAATTAACCACTACCAACAACAAAACGCCTTGTTACAGACTTTAATGAAGGAAATTGAGTTAATGTTTCAAAACAACTTGGATACCGTTAACATCATTAACCAGATTAACGAACACTCGCTGTTAATTGCACAAAAAACCCAACTACTCAACCAGGAAAATGCGCTGACCGAATTCATTGATTATGAGAAGTTGTTTCGCTTAATGAGTGAAGCGATGAAGTTATTGGATGGATTGAAAAATCTGTTAAACGAGCTGTTTGCGCTGTCAGCTAATAAGTTTGATGATTACCGCTTTTTTGTCTATACCTTAGATGACTTTCGTTTTAAGTTTTTCCAAATTGAAAACTTAATTGCGAATGAAGAGTTAATTATTTCCGAAAAGACTTTAAAGATTATTAGTCAAGCTAAGCACCAGTTTGATGATATTTTTACCCAAGCGAAAAATGACTATGAGGGTGCTTTTAGCTATTTAAACGAAAAGGTGAGGTTCTTCCAAAACCAACTAACCCATGTGATTGTTGATGTAGTAGGTTTAATGAACCTGAGATCAATGTGTAAACATACCTTTATCTTTGCCAATAAGTACCGCCAAGAAAGCCCCCAAATTAACGAAAGCTTGGAAACGTTGACCCAGTTTTACCAACAGCAGAATTATTCGGAAACTTTGAGTGGTTTAATTAGTTTGTTGGGTAAAATTAAATCATCTGCTAAACAACACCACCTTGATTTAAACTAA", "aa": "MVVIAALLGSLAVLAFLFYLWYLTIFIIVHKNQQYLEQTKALYNKLKLSNFNSVIVPFQLLRSEKKALEQPVKLLKSFQESFNSEINSALEQLLVFSEPKALYNCFWFNRNIKLLRKNLQGLHDKQQRYVKLTKNAIAYFDSSYDTLVFYRQAFCLLVSFINDFLVHKYDSLFYLNIINRISLLFKDVELHIKNKDVKQQNQALNKLHNNLAQTIITASRQYFFDVKVGYLNYHFQILSQKVTQLRSMKNKAINSQEVAKFQELITNIGATLAECSQALGNINLALCEQRINQAKEQIDVLSNAVSVKEKAISLVVSNVDSFLKSINHYQQQNALLQTLMKEIELMFQNNLDTVNIINQINEHSLLIAQKTQLLNQENALTEFIDYEKLFRLMSEAMKLLDGLKNLLNELFALSANKFDDYRFFVYTLDDFRFKFFQIENLIANEELIISEKTLKIISQAKHQFDDIFTQAKNDYEGAFSYLNEKVRFFQNQLTHVIVDVVGLMNLRSMCKHTFIFANKYRQESPQINESLETLTQFYQQQNYSETLSGLISLLGKIKSSAKQHHLDLN", "aa_hexdigest": "e2b370d0b231a36cccfafeab8a83ce7b", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000139743", "ncbi_nrp_id": "WP_010874953.1", "uniref100_id": "UniRef100_Q50333", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139743", "RefSeq:WP_010874953.1", "UniRef:UniRef100_Q50333" ] }, "ips": { "uniref100_id": "UniRef100_Q50333", "uniref90_id": "UniRef90_Q50333" }, "psc": { "uniref90_id": "UniRef90_Q50333", "product": "Uncharacterized protein MG397 homolog", "uniref50_id": "UniRef50_P47637", "go_ids": [ "GO:0016020" ] }, "pscc": { "uniref50_id": "UniRef50_P47637", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47637" ], "product": "Uncharacterized protein MG397" }, "genes": [], "id": "APKECPOBEN_1270", "locus": "GCA002090215_01270" }, { "type": "cds", "sequence": "contig_1", "start": 719186, "stop": 719587, "strand": "-", "frame": 3, "gene": "atpC", "product": "F0F1 ATP synthase subunit epsilon", "db_xrefs": [ "COG:C", "COG:COG0355", "EC:7.1.2.2", "GO:0005524", "GO:0005886", "GO:0045261", "GO:0046933", "RefSeq:WP_010874954.1", "SO:0001217", "UniParc:UPI00001264FB", "UniRef:UniRef100_Q50332", "UniRef:UniRef50_P47638", "UniRef:UniRef90_Q50332" ], "nt": "ATGCAACCATTGCGCTTTTTAGTCTTAAGTCCCAGTGGTATTAAACTCGATCAAGCCATTATTAGTGCGCAAGTCAAAACCACTAATGGTTATCTGGGTTTGAACTATAACCGTGCGCCCTTAATTGCGGCCGTACAATCGCACTTTTGCAAAATTCTGTTTGCCAATAACACGCGGCGCAATGCCATCATAGGAGCGGGGTTAGTGCTCATTAAGAAAACTGAGGCCAAGATCTTTACCGAGAACTTTGTCTTTGAGGATGAAATAGATCTGGAAGCGACTTTGAAGCGTAAGGCCGAGTTAGAGCGGCAAATGAACCACTCGAAGGACTTAAAGCTCAACATTAAGATTGAACAAAACTTAATGTTTGAACTGTTAAAGCTTTCCAATAAGCAACGCTAA", "aa": "MQPLRFLVLSPSGIKLDQAIISAQVKTTNGYLGLNYNRAPLIAAVQSHFCKILFANNTRRNAIIGAGLVLIKKTEAKIFTENFVFEDEIDLEATLKRKAELERQMNHSKDLKLNIKIEQNLMFELLKLSNKQR", "aa_hexdigest": "cf26a0ac06b3935c1b93e9248ddb46c2", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001264FB", "ncbi_nrp_id": "WP_010874954.1", "uniref100_id": "UniRef100_Q50332", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001264FB", "RefSeq:WP_010874954.1", "UniRef:UniRef100_Q50332" ] }, "ips": { "uniref100_id": "UniRef100_Q50332", "uniref90_id": "UniRef90_Q50332" }, "psc": { "uniref90_id": "UniRef90_Q50332", "gene": "atpC", "product": "F0F1 ATP synthase subunit epsilon", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_P47638", "cog_id": "COG0355", "cog_category": "C", "go_ids": [ "GO:0005886", "GO:0045261", "GO:0005524", "GO:0046933" ] }, "pscc": { "uniref50_id": "UniRef50_P47638", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47638" ], "product": "ATP synthase epsilon chain" }, "genes": [ "atpC" ], "id": "APKECPOBEN_1271", "locus": "GCA002090215_01271" }, { "type": "cds", "sequence": "contig_1", "start": 719590, "stop": 721017, "strand": "-", "frame": 1, "gene": "atpD", "product": "F0F1 ATP synthase subunit beta", "db_xrefs": [ "COG:C", "COG:COG0055", "EC:7.1.2.2", "GO:0005524", "GO:0005886", "GO:0015986", "GO:0016887", "GO:0045261", "GO:0046933", "GO:0046961", "RefSeq:WP_010874955.1", "SO:0001217", "UniParc:UPI0000126461", "UniRef:UniRef100_Q50331", "UniRef:UniRef50_P50002", "UniRef:UniRef90_Q50331" ], "nt": "ATGAAAAAGGAAAACATTACATACGGTAAGGTCCACCAAGTGATTGGCCCGGTAGTTGATGTCATCTTTACGGAAAGTAGTCAGTTACCCCGCATTTACGATTGTTTGAGTGTTAAGTTAGCTGGGGAAGAACTGTTTTTGGAAGCCGCGCAGCTAATAGGCGATGACATTGTGCGCTGCATTGCTTTGGGTCCAACTGAAGGGTTAGCACGCAACGAAAAGGTGACTAACTATAACCACCCGATTGAAGTCCCAGTTGGCAAAAACGTCTTGGGCCGGATGTTTAATGTTTTGGGTAAACCCATTGACGGGAAGGAGGAGTTACCCAAAAAACCACAACTCCCGATTCACCGCAAACCACCTTCGTTTGATGACCAGTCCAATACGCTAGAAATCTTTGAAACAGGGATTAAGGTGATTGACTTGCTCACTCCCTATGCCCGTGGTGGTAAGATTGGTTTATTTGGTGGTGCTGGTGTTGGTAAAACGGTTTTAGTGCAAGAGTTAATCCACAACATTGCTAAGGAACACTCTGGTTTAAGTGTGTTTGCTGGTGTGGGGGAACGCACCCGGGAAGGTAATGATCTCTACTATGAAATGATCCAGGGTGGGGTGATTGACAAAACTGCTTTAGTGTTTGGTCAGATGAACGAACCCCCAGGAGCACGGATGCGGGTCGCTTTAACGGCGCTTACTATGGCCGAATACTTCCGTGACCATGACAACCAGGATGTCTTGCTCTTCATTGACAACATCTTCCGTTTTACCCAAGCTGGTAGTGAGGTATCAGCACTGTTAGGTCGGATGCCATCAGCCGTGGGTTACCAACCAACGTTGGCGACTGAAATGGGGCAATTACAAGAGCGGATTGCTTCCACTAAAACCGGTTCGATTACCTCGGTGCAAGCGATCTATGTGCCAGCTGATGACTTGACTGACCCCGCTCCGGCTACCACCTTTACCCACCTGGATGCTAAAACGGTGTTAGACCGGAACATAGCAGCGTTGGGAATTTTTCCAGCGATCAATCCCTTAGAGTCTACCAGTCGCTTATTGGATCCTAATATTGTCGGGATTAACCACTATAAGGTAGCGTTAGGGGTGCAAAACATCTTACAGCGCTTTGCGGAACTACAAGACATTATTGCCATCTTGGGAATTGATGAGTTAGCGGATGAGGACAAGATCATTGTGGAACGGGCACGCCGGATCCGCAACTTCCTCTCCCAACCGTTCTTTGTAGCGGAAAAGTTCTCGGGGATTGCTGGGAAGTATGTCCCTTTAAGTGACACGATCCAATCGTTTAAGGAAATCTTGGACGGTAAGCATGATGATCTCCCCGAACAGGCCTTCTTCTTTGTTGGTACCATTCAGGAAGCAGTGGAGAAAGCGAAGCGGCTTAAAAAAGCTACTGTTGAGGAGAAATAA", "aa": "MKKENITYGKVHQVIGPVVDVIFTESSQLPRIYDCLSVKLAGEELFLEAAQLIGDDIVRCIALGPTEGLARNEKVTNYNHPIEVPVGKNVLGRMFNVLGKPIDGKEELPKKPQLPIHRKPPSFDDQSNTLEIFETGIKVIDLLTPYARGGKIGLFGGAGVGKTVLVQELIHNIAKEHSGLSVFAGVGERTREGNDLYYEMIQGGVIDKTALVFGQMNEPPGARMRVALTALTMAEYFRDHDNQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQPTLATEMGQLQERIASTKTGSITSVQAIYVPADDLTDPAPATTFTHLDAKTVLDRNIAALGIFPAINPLESTSRLLDPNIVGINHYKVALGVQNILQRFAELQDIIAILGIDELADEDKIIVERARRIRNFLSQPFFVAEKFSGIAGKYVPLSDTIQSFKEILDGKHDDLPEQAFFFVGTIQEAVEKAKRLKKATVEEK", "aa_hexdigest": "a5f6f0fc6f01080bf3da9392d68c15c1", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000126461", "ncbi_nrp_id": "WP_010874955.1", "uniref100_id": "UniRef100_Q50331", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000126461", "RefSeq:WP_010874955.1", "UniRef:UniRef100_Q50331" ] }, "ips": { "uniref100_id": "UniRef100_Q50331", "uniref90_id": "UniRef90_Q50331" }, "psc": { "uniref90_id": "UniRef90_Q50331", "gene": "atpD", "product": "F0F1 ATP synthase subunit beta", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_P50002", "cog_id": "COG0055", "cog_category": "C", "go_ids": [ "GO:0005524", "GO:0005886", "GO:0015986", "GO:0016887", "GO:0045261", "GO:0046933", "GO:0046961" ] }, "pscc": { "uniref50_id": "UniRef50_P50002", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P50002" ], "product": "ATP synthase subunit beta, sodium ion specific" }, "genes": [ "atpD" ], "id": "APKECPOBEN_1272", "locus": "GCA002090215_01272" }, { "type": "cds", "sequence": "contig_1", "start": 721017, "stop": 721451, "strand": "-", "frame": 2, "gene": "atpG", "product": "ATP synthase F1 subunit gamma", "db_xrefs": [ "COG:C", "COG:COG0224", "EC:7.1.2.2", "GO:0005524", "GO:0005886", "GO:0015986", "GO:0042777", "GO:0045261", "GO:0046933", "SO:0001217", "UniRef:UniRef50_P47640", "UniRef:UniRef90_Q50330" ], "nt": "TTGAGCTTTGATTACTGTCAACAGGTGTCCGACCAAATTATGGAGCAGTTTGATTTACACCAATTAGACCGTATTTGCATCATTTACACCCAGTTTAAAAACCCCTTAATTCAACACGCCAACAGCTTCCAGGTTTTTCCCTTTGATGTAGCGATGTTTAAGGCCTTCAACCCGGTCAAAATGGAACAAAAGCTCGACTTTGAACCCGATGAGGAGACAATTATTAAGCTCATTAGTCCCCAGTTCTTTGACATTGCGTTATACGGTGGGTTGGTAGAGACAAAGTTGTGTGAGTCAGCATCAAGACAAAACGCCATGGATGCCGCTGCTAAGAATGCCAAGGATCTGTATGAAAAGTACAGTATTCAATTTAATAAGTTAAGACAAAACTCTATTACCCAAGAAATTATTGAAATCATAGGAGGTATTAGTTAA", "aa": "MSFDYCQQVSDQIMEQFDLHQLDRICIIYTQFKNPLIQHANSFQVFPFDVAMFKAFNPVKMEQKLDFEPDEETIIKLISPQFFDIALYGGLVETKLCESASRQNAMDAAAKNAKDLYEKYSIQFNKLRQNSITQEIIEIIGGIS", "aa_hexdigest": "118938d06364c706d2bc1fb92b85c8bf", "start_type": "TTG", "rbs_motif": null, "genes": [ "atpG" ], "truncated": "3-prime", "pseudogene": { "start": 721017, "stop": 721751, "observations": { "mutation": [ 721511 ], "stop": [ 721511 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q50330", "query_cov": 1.0, "subject_cov": 0.5161290322580645, "identity": 0.993, "score": 286.0, "evalue": 9.69e-96, "gene_start": 1, "gene_end": 144, "reference_start": 136, "reference_end": 279, "reference_sequence": "MAFIQEIKRKMTTVQSTIKITNAMKMVSRAKFVRFKKQFKEVNYFFNEFYKAVGQVVLSLKKMPKPLENPKTLWVMMSSSLGLCGQHNTNMNKLLQTKFQPGDKIFFLGRKNQSFWNKGDTDNPTVGYVDIQDRDLSFDYCQQVSDQIMEQFDLHQLDRICIIYTQFKNPLIQHANSFQVFPFDVAMFKAFNPVKMEQKLDFEPDEETIIKLISPQFFDIALYGGLVETKLCESASRQNAMDAAAKNAKDLYEKYSIQFNKLRQNSITQEIIEIIGGIS" }, "paralog": false, "identity": 0.9959016393442623, "score": 492.0, "evalue": 2.64e-179, "description": "Internal stop codon at 721511. Nonsense mutation around 721511.", "psc": { "uniref90_id": "UniRef90_Q50330", "gene": "atpG", "product": "ATP synthase F1 subunit gamma", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_P47640", "cog_id": "COG0224", "cog_category": "C", "go_ids": [ "GO:0005524", "GO:0005886", "GO:0015986", "GO:0042777", "GO:0045261", "GO:0046933" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q50330", "EC:7.1.2.2", "UniRef:UniRef50_P47640", "COG:COG0224", "COG:C", "GO:0005524", "GO:0005886", "GO:0015986", "GO:0042777", "GO:0045261", "GO:0046933" ] }, "pscc": { "uniref50_id": "UniRef50_P47640", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47640" ], "product": "ATP synthase gamma chain" } }, "id": "APKECPOBEN_1273", "locus": "GCA002090215_01273" }, { "type": "cds", "sequence": "contig_1", "start": 721509, "stop": 721856, "strand": "-", "frame": 2, "gene": "atpG", "product": "ATP synthase F1 subunit gamma", "db_xrefs": [ "COG:C", "COG:COG0224", "EC:7.1.2.2", "GO:0005524", "GO:0005886", "GO:0015986", "GO:0042777", "GO:0045261", "GO:0046933", "SO:0001217", "UniRef:UniRef50_P47640", "UniRef:UniRef90_Q50330" ], "nt": "ATGGCCTTTATCCAGGAAATTAAGCGCAAAATGACCACGGTGCAGTCCACCATTAAGATTACTAACGCAATGAAAATGGTGTCCCGTGCTAAGTTTGTCCGCTTTAAAAAACAGTTTAAGGAAGTTAACTACTTCTTCAATGAGTTTTATAAGGCCGTTGGGCAAGTAGTTCTCAGCTTAAAGAAGATGCCCAAGCCCCTGGAAAATCCCAAAACCCTGTGGGTGATGATGAGTTCATCGTTGGGTCTTTGTGGACAGCACAACACTAACATGAACAAGTTGTTGCAAACGAAGTTCCAACCCGGTGACAAAATCTTCTTTTTGGGGCGCAAAAACCAGTCTTTTTGA", "aa": "MAFIQEIKRKMTTVQSTIKITNAMKMVSRAKFVRFKKQFKEVNYFFNEFYKAVGQVVLSLKKMPKPLENPKTLWVMMSSSLGLCGQHNTNMNKLLQTKFQPGDKIFFLGRKNQSF", "aa_hexdigest": "d84c18f22f3a98013e8fd9bae2cc8332", "start_type": "ATG", "rbs_motif": null, "genes": [ "atpG" ], "truncated": "5-prime", "pseudogene": { "start": 721206, "stop": 721856, "observations": { "mutation": [ 721511 ], "stop": [ 721511 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q50330", "query_cov": 1.0, "subject_cov": 0.4121863799283154, "identity": 1.0, "score": 228.0, "evalue": 1.27e-73, "gene_start": 1, "gene_end": 115, "reference_start": 1, "reference_end": 115, "reference_sequence": "MAFIQEIKRKMTTVQSTIKITNAMKMVSRAKFVRFKKQFKEVNYFFNEFYKAVGQVVLSLKKMPKPLENPKTLWVMMSSSLGLCGQHNTNMNKLLQTKFQPGDKIFFLGRKNQSFWNKGDTDNPTVGYVDIQDRDLSFDYCQQVSDQIMEQFDLHQLDRICIIYTQFKNPLIQHANSFQVFPFDVAMFKAFNPVKMEQKLDFEPDEETIIKLISPQFFDIALYGGLVETKLCESASRQNAMDAAAKNAKDLYEKYSIQFNKLRQNSITQEIIEIIGGIS" }, "paralog": false, "identity": 0.9953703703703703, "score": 436.0, "evalue": 1.44e-157, "description": "Internal stop codon at 721511. Nonsense mutation around 721511.", "psc": { "uniref90_id": "UniRef90_Q50330", "gene": "atpG", "product": "ATP synthase F1 subunit gamma", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_P47640", "cog_id": "COG0224", "cog_category": "C", "go_ids": [ "GO:0005524", "GO:0005886", "GO:0015986", "GO:0042777", "GO:0045261", "GO:0046933" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q50330", "EC:7.1.2.2", "UniRef:UniRef50_P47640", "COG:COG0224", "COG:C", "GO:0005524", "GO:0005886", "GO:0015986", "GO:0042777", "GO:0045261", "GO:0046933" ] }, "pscc": { "uniref50_id": "UniRef50_P47640", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47640" ], "product": "ATP synthase gamma chain" } }, "id": "APKECPOBEN_1274", "locus": "GCA002090215_01274" }, { "type": "cds", "sequence": "contig_1", "start": 721856, "stop": 723412, "strand": "-", "frame": 3, "gene": "atpA", "product": "F0F1 ATP synthase subunit alpha", "db_xrefs": [ "COG:C", "COG:COG0056", "EC:7.1.2.2", "GO:0005524", "GO:0005886", "GO:0015986", "GO:0043531", "GO:0045261", "GO:0046933", "GO:0046961", "RefSeq:WP_010874957.1", "SO:0001217", "UniParc:UPI00001263FA", "UniRef:UniRef100_Q50329", "UniRef:UniRef50_P33252", "UniRef:UniRef90_Q50329" ], "nt": "ATGGCAGACAAATTAAATGAATATGTAGCACTCATTAAAAATGAGATTAAGAAGTATTCCAAACAGATCTTTAACAGTGAAATTGGCAAGGTTATTAGTGTCGCTGATGGCATTGCTAAGGTCAGTGGTTTGGAAAACGCACTCTTAAACGAGTTAATTGAGTTTGAAAACAACGTGCAGGGGATTGCGCTTAACCTAGAGCAAAACACGGTTGGGGTGGCGTTGTTTGGTGATTACTCTAAAATCCGTGAAGGTAGTACGGCTAAACGGACCCACAACGTGATGCAAACTCCCGTGGGTGATGTAATGTTGGGCCGGATTGTCAATGCCCTGGGAGAACCGGTGGATGGTCGTGGTCCGATTAAGGCTGAAGAGTTTGACCAAGTGGAGAAGATTGCCCCTGGGGTAATGACCCGCAAAACCGTTAACCAACCACTGGAAACTGGAATCTTGACGATTGATGCGTTGTTTCCGATTGGTAAGGGACAACGGGAGTTAATTGTGGGGGACCGCCAAACTGGTAAGACCTCAATTGCAATTGACACCATTATTAACCAACGTGGTAAGGACGTGTACTGTGTCTATGTAGCGATGGGACAAAAGAATTCCTCGGTAGCGCAAATTGTCCACCAGTTAGAGGTAACTGATTCGATGAAGTACACCACGGTGGTGTGTGCTACAGCCAGTGATCCCGCTTCGATGATCTACCTCACGCCCTTTACTGGCATTACCATTGCTGAGTACTGGCTCAAACAGGGTAAGGATGTTCTAATTGTGTTTGATGATTTGTCCAAGCACGCGATTGCTTACCGTACCTTATCCTTACTGTTACGCCGACCACCAGGACGGGAAGCCTTTCCTGGGGATGTGTTCTACTTGCACTCGCGTTTACTGGAACGTGCTTGCCGTTTAAACGAGGAGCATGGTGGTGGCTCGATTACCGCTTTACCTATTATTGAAACGCAAGCTGGGGACATTTCCGCTTACATTCCGACCAACGTCATTTCGATTACCGATGGTCAACTGTTCATGGTGTCAAACCTGTTTAACTCCGGTCAACGCCCTGCCATCCACGTGGGGTTATCGGTGTCGCGGGTTGGTAGTGCAGCGCAAATTAAAGCAATTAAACAACAAACCGGGAGCTTAAAGTTAGAGCTAGCGCAGTACAGTGAATTAGACAGCTTTAGTCAGTTTGGTAGTGACTTGGACGAAAATACCAAGCAAATCTTGGAACGCGGTAAGCGGGTAATGGAGATGATTAAACAACCCAACGGTAAACCTTACTCACAAACCCATGAAGCGTTATTCTTGTTTGCGATTGCCAAGTCCTTCATTAAGTTTATTCCACTGGATTACATTGCTAAATTTAAGCAACGCATTATGGAGGAATTTGACAAGGAACACCCGATTTACAAGGAAATAGCGACCCAAAAGAGCTTTAGTGAAGCGTTGGAAGCACAAACTAACACTGCTTTTAAGGCACTAGTAAAACGCTTTGTTAGTGCTTTACCAGATTACGACATCACTAAATACGGAACGATGGAGGAGTTGGAATAA", "aa": "MADKLNEYVALIKNEIKKYSKQIFNSEIGKVISVADGIAKVSGLENALLNELIEFENNVQGIALNLEQNTVGVALFGDYSKIREGSTAKRTHNVMQTPVGDVMLGRIVNALGEPVDGRGPIKAEEFDQVEKIAPGVMTRKTVNQPLETGILTIDALFPIGKGQRELIVGDRQTGKTSIAIDTIINQRGKDVYCVYVAMGQKNSSVAQIVHQLEVTDSMKYTTVVCATASDPASMIYLTPFTGITIAEYWLKQGKDVLIVFDDLSKHAIAYRTLSLLLRRPPGREAFPGDVFYLHSRLLERACRLNEEHGGGSITALPIIETQAGDISAYIPTNVISITDGQLFMVSNLFNSGQRPAIHVGLSVSRVGSAAQIKAIKQQTGSLKLELAQYSELDSFSQFGSDLDENTKQILERGKRVMEMIKQPNGKPYSQTHEALFLFAIAKSFIKFIPLDYIAKFKQRIMEEFDKEHPIYKEIATQKSFSEALEAQTNTAFKALVKRFVSALPDYDITKYGTMEELE", "aa_hexdigest": "92e9957249aafa0a41b1b91cd85c96b6", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001263FA", "ncbi_nrp_id": "WP_010874957.1", "uniref100_id": "UniRef100_Q50329", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001263FA", "RefSeq:WP_010874957.1", "UniRef:UniRef100_Q50329" ] }, "ips": { "uniref100_id": "UniRef100_Q50329", "uniref90_id": "UniRef90_Q50329" }, "psc": { "uniref90_id": "UniRef90_Q50329", "gene": "atpA", "product": "F0F1 ATP synthase subunit alpha", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_P33252", "cog_id": "COG0056", "cog_category": "C", "go_ids": [ "GO:0005524", "GO:0005886", "GO:0015986", "GO:0043531", "GO:0045261", "GO:0046933", "GO:0046961" ] }, "pscc": { "uniref50_id": "UniRef50_P33252", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P33252" ], "product": "ATP synthase subunit alpha" }, "genes": [ "atpA" ], "id": "APKECPOBEN_1275", "locus": "GCA002090215_01275" }, { "type": "cds", "sequence": "contig_1", "start": 723415, "stop": 723951, "strand": "-", "frame": 1, "gene": "atpH", "product": "ATP synthase F1 subunit delta", "db_xrefs": [ "COG:C", "COG:COG0712", "EC:7.1.2.2", "GO:0005886", "GO:0015986", "GO:0045261", "GO:0046933", "RefSeq:WP_010874958.1", "SO:0001217", "UniParc:UPI00001264AB", "UniRef:UniRef100_Q50328", "UniRef:UniRef50_P47642", "UniRef:UniRef90_Q50328" ], "nt": "ATGATTAACGCGCAGGCTTTTGGAATGGCACTCTTCCAACTTAGTGAAGAGGAAAAGAAGGTTAAGAAGGTCTATGACCAGAGCCATGATTTTTTAAAACTAGCGCGTAACTTTAAAGATGGATCACTAGCTGCTCTACTCAATGCTTATACTTTAACCAAAAAGGAAAAGCTTAAGCTAATAGATAAGCTGTTTAAAAACCACTTTTGTGACCTCTTTGTTGACTTTTTAAAAACGATTGTGCTCAAGGGCTACTTTAACCTAGTCGAGCAAGCCTTAAAGTACTTCTTTGATTGTGTTGAGAATGAAAAACACGTCCAGTTCATTAGGATCATTACCGCTTTTGAACTGAGTGCGCCCCAACTCAAGCGAATCGTAGCGGCAATGGAAAAGAAGCTCAATTCCAAGATTGTTTACAAAACCGAAATTGACAAGAGCTTGATTTCGGGTATCCGAATTGAATCGAGCGCCCAGTTATTTGAAAAGAATATCCGTGATCAACTATCACGTTTAATGGAACAGTTTAAAGGGAATTAA", "aa": "MINAQAFGMALFQLSEEEKKVKKVYDQSHDFLKLARNFKDGSLAALLNAYTLTKKEKLKLIDKLFKNHFCDLFVDFLKTIVLKGYFNLVEQALKYFFDCVENEKHVQFIRIITAFELSAPQLKRIVAAMEKKLNSKIVYKTEIDKSLISGIRIESSAQLFEKNIRDQLSRLMEQFKGN", "aa_hexdigest": "80aeac672ecca882a1b69f079bab2327", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001264AB", "ncbi_nrp_id": "WP_010874958.1", "uniref100_id": "UniRef100_Q50328", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001264AB", "RefSeq:WP_010874958.1", "UniRef:UniRef100_Q50328" ] }, "ips": { "uniref100_id": "UniRef100_Q50328", "uniref90_id": "UniRef90_Q50328" }, "psc": { "uniref90_id": "UniRef90_Q50328", "gene": "atpH", "product": "ATP synthase F1 subunit delta", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_P47642", "cog_id": "COG0712", "cog_category": "C", "go_ids": [ "GO:0005886", "GO:0015986", "GO:0045261", "GO:0046933" ] }, "pscc": { "uniref50_id": "UniRef50_P47642", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47642" ], "product": "ATP synthase subunit delta" }, "genes": [ "atpH" ], "id": "APKECPOBEN_1276", "locus": "GCA002090215_01276" }, { "type": "cds", "sequence": "contig_1", "start": 723944, "stop": 724318, "strand": "-", "frame": 3, "gene": "atpF", "product": "F0F1 ATP synthase subunit B", "db_xrefs": [ "COG:C", "COG:COG0711", "EC:7.1.2.2", "GO:0005886", "GO:0015078", "GO:0015986", "GO:0045263", "GO:0046933", "GO:0046961", "SO:0001217", "UniRef:UniRef50_Q50327", "UniRef:UniRef90_Q50327" ], "nt": "TTGGAAGAGCAAGTCAACCAGGCCAACAGCTTAGAACAACAAGCTCAAGCATTACTCCAACAAGCCAACCAACGCCATGAAAACTCACTGGTAGTTGCTAAGGAAATTGTAGACCAGGCCAACTATGAGGCGTTGCAACTTAAAAGTGAAATCGAAAAGAAGGCTAACCGGCAAGCTAACTTAATGATCTTCCAAGCGCGTCAGGAAATTGAAAAGGAAAAGCGCTTGATCCAAGAACAATCCTTAAAGGAAAGTGTCGAGCTAGCCATGTTAGCTGCTAAGGAATTGATTATCAAAAAGGTGGATGTCAAAGCCGACAAGGCCTTTATTGAAGAGTTTATCCGCGAACTAGAAGCAGAGGACGACCATGATTAA", "aa": "MEEQVNQANSLEQQAQALLQQANQRHENSLVVAKEIVDQANYEALQLKSEIEKKANRQANLMIFQARQEIEKEKRLIQEQSLKESVELAMLAAKELIIKKVDVKADKAFIEEFIRELEAEDDHD", "aa_hexdigest": "0a202e2ebfc50ed763cad45145aeba80", "start_type": "TTG", "rbs_motif": null, "genes": [ "atpF" ], "truncated": "3-prime", "pseudogene": { "start": 723944, "stop": 724567, "observations": { "mutation": [ 724360 ], "stop": [ 724360 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q50327", "query_cov": 1.0, "subject_cov": 0.5990338164251208, "identity": 0.992, "score": 210.0, "evalue": 3.69e-67, "gene_start": 1, "gene_end": 124, "reference_start": 84, "reference_end": 207, "reference_sequence": "MKLRATFVFKTTLVALSFALFALFLVSCTENVKEIKSESVINELFPNLWVFLAHLLAFVILLFLLLFLFWKPTQKFLNQRKALLEEQVNQANSLEQQAQALLQQANQRHENSLVVAKEIVDQANYEALQLKSEIEKKANRQANLMIFQARQEIEKEKRLIQEQSLKESVELAMLAAKELIIKKVDVKADKAFIEEFIRELEAEDDHD" }, "paralog": false, "identity": 0.9951690821256038, "score": 386.0, "evalue": 4.64e-139, "description": "Internal stop codon at 724360. Nonsense mutation around 724360.", "psc": { "uniref90_id": "UniRef90_Q50327", "gene": "atpF", "product": "F0F1 ATP synthase subunit B", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_Q50327", "cog_id": "COG0711", "cog_category": "C", "go_ids": [ "GO:0005886", "GO:0015078", "GO:0015986", "GO:0045263", "GO:0046933", "GO:0046961" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q50327", "EC:7.1.2.2", "UniRef:UniRef50_Q50327", "COG:COG0711", "COG:C", "GO:0005886", "GO:0015078", "GO:0015986", "GO:0045263", "GO:0046933", "GO:0046961" ] }, "pscc": { "uniref50_id": "UniRef50_Q50327", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50327" ], "product": "ATP synthase subunit b" } }, "id": "APKECPOBEN_1277", "locus": "GCA002090215_01277" }, { "type": "cds", "sequence": "contig_1", "start": 724358, "stop": 724567, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAACTACGAGCAACTTTTGTATTTAAAACGACCTTAGTTGCGCTTAGTTTTGCCCTGTTTGCCTTGTTTTTAGTTTCCTGTACCGAAAACGTTAAGGAAATTAAAAGTGAATCAGTCATCAACGAACTTTTTCCGAACCTCTGGGTCTTTTTAGCCCACTTGTTGGCCTTTGTGATCCTGTTGTTCTTGTTGTTGTTCCTCTTTTGA", "aa": "MKLRATFVFKTTLVALSFALFALFLVSCTENVKEIKSESVINELFPNLWVFLAHLLAFVILLFLLLFLF", "aa_hexdigest": "5878f6f07991407453cf537e44b74ec1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7955.630299999993, "isoelectric_point": 7.940844917297363 }, "id": "APKECPOBEN_1278", "locus": "GCA002090215_01278" }, { "type": "cds", "sequence": "contig_1", "start": 724570, "stop": 724887, "strand": "-", "frame": 1, "gene": "atpE", "product": "F0F1 ATP synthase subunit C", "db_xrefs": [ "COG:C", "COG:COG0636", "EC:7.1.2.2", "GO:0005886", "GO:0008289", "GO:0045263", "GO:0046933", "RefSeq:WP_010874960.1", "SO:0001217", "UniParc:UPI00001265D2", "UniRef:UniRef100_Q59550", "UniRef:UniRef50_P47644", "UniRef:UniRef90_Q59550" ], "nt": "ATGGAACACGTTAATGAAATTCTCGCCACAGTTGGCCGCATCTTACACGAAACAACAACAGCTAATACCAATGTTGCCAATAAAAGTACCGAACGTTTAGGGGCTTACATTGGGGCTGGGATTACCATGGTCGGTGGTGCGACCGTGGGTCTTGGTCAAGGTTACATCTTTGGGAAAGCGGTTGAAGCCGTAGCGCGGAACCCCGAAGTGGAAAAGCAGGTCTTTAAATTAATCTTTATTGGCTCTGCTATTAGTGAATCGAGTTCCATCTATTCCTTGTTAATAGCCTTCATTCTTATCTTTGTATCGGGCGCTTAA", "aa": "MEHVNEILATVGRILHETTTANTNVANKSTERLGAYIGAGITMVGGATVGLGQGYIFGKAVEAVARNPEVEKQVFKLIFIGSAISESSSIYSLLIAFILIFVSGA", "aa_hexdigest": "db463fb393f3e86270e9f374118cb4dc", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001265D2", "ncbi_nrp_id": "WP_010874960.1", "uniref100_id": "UniRef100_Q59550", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001265D2", "RefSeq:WP_010874960.1", "UniRef:UniRef100_Q59550" ] }, "ips": { "uniref100_id": "UniRef100_Q59550", "uniref90_id": "UniRef90_Q59550" }, "psc": { "uniref90_id": "UniRef90_Q59550", "gene": "atpE", "product": "F0F1 ATP synthase subunit C", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_P47644", "cog_id": "COG0636", "cog_category": "C", "go_ids": [ "GO:0005886", "GO:0045263", "GO:0008289", "GO:0046933" ] }, "pscc": { "uniref50_id": "UniRef50_P47644", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47644" ], "product": "ATP synthase subunit c" }, "genes": [ "atpE" ], "id": "APKECPOBEN_1279", "locus": "GCA002090215_01279" }, { "type": "cds", "sequence": "contig_1", "start": 724890, "stop": 725240, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00119.25" ], "nt": "TTGAGCTTCTTGGGTGAGTTTGCGCCACTGTTCTCGATTTCGCTTCGTTTGTGGGGCAATATCCTTGGGGGAACCTTGATTTTAGCGCTCTTTTATAACTTCTGGTTCTTTGCTTTTTCGACTTTAAGCAATAAGCCACTAGCGCTTAGTTTAGGTGCCATTTTTGCAGGAATTCTCACCCCAGCCCTACATGTATATTTTGATGTAGTAGTGGGTACACTGCAGGGATATGTCTTTGTGATGTTGACTTATAATTATTGGGCAAAAATGCGTAACATTGGCTTGGAAGAAAGTCAAGAAGCAGCGCAGCGTTTGCAAAATTTGGAGGTAGCAAAAGAAATAATTAATTAG", "aa": "MSFLGEFAPLFSISLRLWGNILGGTLILALFYNFWFFAFSTLSNKPLALSLGAIFAGILTPALHVYFDVVVGTLQGYVFVMLTYNYWAKMRNIGLEESQEAAQRLQNLEVAKEIIN", "aa_hexdigest": "965d7137397394ff0a697528c60a6335", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00119.25", "name": "ATP-synt_A", "length": 90, "aa_cov": 0.7413793103448276, "hmm_cov": 0.9, "evalue": 5.649498051197441e-19, "score": 64.29066467285156, "start": 2, "stop": 87 } ], "seq_stats": { "molecular_weight": 13047.127499999991, "isoelectric_point": 5.668689155578614 }, "id": "APKECPOBEN_1280", "locus": "GCA002090215_01280" }, { "type": "cds", "sequence": "contig_1", "start": 725257, "stop": 725469, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGAGTACTTCTTGCCCTTAATCGTAATGTTGAAGGTAAAGCTTTTAAAGAAGGATCACCGTTGGTACCTAATCCCCATAATGACAATTCCCAAAAAGGTGGCTAGCCCTAAAGAAAAGGTAAAGGTCAGTGATGAAGTTGGTGGGGAAATGCCACCTAATAATCCAATCAAGTTACTGGAAACCAGGTACAACAGCAACATTAAAAAGTAA", "aa": "MEYFLPLIVMLKVKLLKKDHRWYLIPIMTIPKKVASPKEKVKVSDEVGGEMPPNNPIKLLETRYNSNIKK", "aa_hexdigest": "9e2672b52be875872eced49919f45324", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8192.855099999997, "isoelectric_point": 9.822548103332519 }, "id": "APKECPOBEN_1281", "locus": "GCA002090215_01281" }, { "type": "cds", "sequence": "contig_1", "start": 726252, "stop": 727586, "strand": "-", "frame": 2, "gene": "eno", "product": "phosphopyruvate hydratase", "db_xrefs": [ "COG:COG0148", "COG:G", "EC:4.2.1.11", "GO:0000015", "GO:0000287", "GO:0004634", "GO:0005576", "GO:0005829", "GO:0006096", "GO:0009986", "KEGG:K01689", "SO:0001217", "UniRef:UniRef50_P75189", "UniRef:UniRef90_P75189" ], "nt": "ATGAGTGCACAAACTGGAACCGATCTTTTCAAGATTGCTGATCTTTTTGCTTACCAAGTTTTTGATTCACGGGGTTTTCCCACTGTTGCTTGTGTTGTGAAGTTAGCTAGTGGTCATACTGGAGAAGCGATGGTTCCATCTGGGGCTTCTACTGGGGAAAAAGAAGCGATTGAACTACGCGATGGTGATCCCAAAGCTTACTTTGGCAAAGGGGTGAGCCAAGCTGTGCAAAACGTTAACCAAACTATTGCCCCTAAACTAATTGGTTTAAATGCGACTGATCAAGCAGCCATTGATGCTTTAATGATCCAACTGGATGGTACCCCCAATAAGGCTAAGTTAGGTGCTAATGCCATTTTGGCAGTTTCTCTAGCTGTGGCTAAAGCAGCTGCTAGCGCCCAAAAGACTTCCTTGTTTAAGTACCTTGCTAACCAGGTCATGGGTCTAAATAAAACCGAGTTTATCCTCACCGTACCAATGCTAAACGTCATTAACGGTGGAGCACATGCTGACAATAACATTGACTTCCAGGAGTTCATGATTATGCCATTGGGTGCTAACTCGATGCACCAAGCATTGAAGATGGCGAGTGAAACCTTCCATGCTTTACAAAAGCTGTTAAAGCAACGGGGTTTAAACACCAATAAGGGTGATGAAGGTGGTTTTGCGCCTAACTTAAAGTTAGCGGAAGAAGCGCTTGATTTAATGGTCGAAGCAATTAAGGCAGCTGGGTACCAACCAGGTAGTGACATTGCGATTGCACTGGATGTAGCAGCGAGTGAGTTTTATGATGACACCACCAAGCGCTATGTCTTCAAAAAAGGAATTAAGGCCAAAATTCTTGACGAGAAGGAATGGAGTTTAACTACTGCCCAAATGATCGCTTACCTCAAGAAGCTTACTGAGCAGTACCCTATTATTTCCATAGAAGATGGTCTTAGCGAACACGACTGGGAAGGTATGGAAACATTAACTAAAACACTGGGTCAACACATTCAAATAGTTGGTGATGATCTGTACTGCACCAATCCAGCAATTGCTGAAAAAGGTGTTGCTCACAAAGCTACCAACTCGATCTTAATTAAACTCAACCAAATTGGTACCCTTACAGAAACAATTAAAGCGATTAACATTGCCAAGGACGCTAACTGGAGTCAGGTAATTTCCCACCGTAGTGGGGAAACCGAAGACACGACAATTGCTGATCTAGCAGTAGCGGCTTGCACTGGTCAAATTAAAACTGGTTCGATGTCCCGCTCCGAACGAATTGCCAAATACAATCGCTTATTGCAAATTGAACTGGAATTGGGCAATAATGCTAAGTACTTAGGTTGA", "aa": "MSAQTGTDLFKIADLFAYQVFDSRGFPTVACVVKLASGHTGEAMVPSGASTGEKEAIELRDGDPKAYFGKGVSQAVQNVNQTIAPKLIGLNATDQAAIDALMIQLDGTPNKAKLGANAILAVSLAVAKAAASAQKTSLFKYLANQVMGLNKTEFILTVPMLNVINGGAHADNNIDFQEFMIMPLGANSMHQALKMASETFHALQKLLKQRGLNTNKGDEGGFAPNLKLAEEALDLMVEAIKAAGYQPGSDIAIALDVAASEFYDDTTKRYVFKKGIKAKILDEKEWSLTTAQMIAYLKKLTEQYPIISIEDGLSEHDWEGMETLTKTLGQHIQIVGDDLYCTNPAIAEKGVAHKATNSILIKLNQIGTLTETIKAINIAKDANWSQVISHRSGETEDTTIADLAVAACTGQIKTGSMSRSERIAKYNRLLQIELELGNNAKYLG", "aa_hexdigest": "bafd65c3ff0d85920093de9bf30d0326", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75189", "query_cov": 1.0, "subject_cov": 0.9568965517241379, "identity": 1.0, "score": 859.0, "evalue": 2.48e-314, "valid": true, "gene": "eno", "product": "phosphopyruvate hydratase", "ec_ids": [ "4.2.1.11" ], "uniref50_id": "UniRef50_P75189", "cog_id": "COG0148", "cog_category": "G", "kegg_orthology_id": "K01689", "go_ids": [ "GO:0000015", "GO:0000287", "GO:0004634", "GO:0005576", "GO:0005829", "GO:0006096", "GO:0009986" ] }, "pscc": { "uniref50_id": "UniRef50_P75189", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75189" ], "product": "Enolase" }, "genes": [ "eno" ], "id": "APKECPOBEN_1282", "locus": "GCA002090215_01282" }, { "type": "cds", "sequence": "contig_1", "start": 727641, "stop": 728114, "strand": "+", "frame": 3, "gene": "msrA", "product": "Peptide methionine sulfoxide reductase MsrA", "db_xrefs": [ "COG:COG0225", "COG:O", "EC:1.8.4.11", "GO:0005737", "GO:0008113", "GO:0034599", "GO:0036211", "GO:0036456", "RefSeq:WP_010874964.1", "SO:0001217", "UniParc:UPI000012F657", "UniRef:UniRef100_P75188", "UniRef:UniRef50_P75188", "UniRef:UniRef90_P75188" ], "nt": "ATGAAACAAATCTATTTTGGTGGTGGCTGTTTTTGGGGTACCCAAAAGTATTTTGATCTGATTAAGGGTGTCCAAAAGACCAGTGTGGGCTACCTCAATTCCAACATGAAAAATCCCACTTACGAACAAGTATGTAGTGGGCAGACCAACGCAGTGGAAGCAGTGTTCGTTGAATATGATGAAAACAAGGTAAGCTTAAACGAACTTATTGATGCTTTCTTTAAGGTCATTGATCCCACCATTAGAAACCGTCAGGGTAATGATATTGGTACCCAGTACCGTACCGGAGTTTATTGGGTAGATCCCCAAGACGAGCAGTTAATTACGCAAAAGTTTAGGGAACTACAGGCCAATTATCCAAAACCAATTGTGACGGAAAACCGTGCAATGGAAAACTATTTTTTAGCAGAAGAGTACCACCAGGATTACTTAAAGAAAAATCCTCATGGTTACTGTCACATTAAATTTGACTAG", "aa": "MKQIYFGGGCFWGTQKYFDLIKGVQKTSVGYLNSNMKNPTYEQVCSGQTNAVEAVFVEYDENKVSLNELIDAFFKVIDPTIRNRQGNDIGTQYRTGVYWVDPQDEQLITQKFRELQANYPKPIVTENRAMENYFLAEEYHQDYLKKNPHGYCHIKFD", "aa_hexdigest": "a6f32d55a9980d1a2810341e441f7a7f", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000012F657", "ncbi_nrp_id": "WP_010874964.1", "uniref100_id": "UniRef100_P75188", "db_xrefs": [ "SO:0001217", "UniParc:UPI000012F657", "RefSeq:WP_010874964.1", "UniRef:UniRef100_P75188" ] }, "ips": { "uniref100_id": "UniRef100_P75188", "uniref90_id": "UniRef90_P75188" }, "psc": { "uniref90_id": "UniRef90_P75188", "gene": "msrA", "product": "Peptide methionine sulfoxide reductase MsrA", "ec_ids": [ "1.8.4.11" ], "uniref50_id": "UniRef50_P75188", "cog_id": "COG0225", "cog_category": "O", "go_ids": [ "GO:0005737", "GO:0036456", "GO:0008113", "GO:0034599", "GO:0036211" ] }, "pscc": { "uniref50_id": "UniRef50_P75188", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75188" ], "product": "Peptide methionine sulfoxide reductase MsrA" }, "genes": [ "msrA" ], "id": "APKECPOBEN_1283", "locus": "GCA002090215_01283" }, { "type": "cds", "sequence": "contig_1", "start": 728111, "stop": 728512, "strand": "-", "frame": 3, "gene": "phoU", "product": "phosphate signaling complex protein PhoU", "db_xrefs": [ "COG:COG0704", "COG:P", "GO:0006817", "GO:0030643", "GO:0045936", "SO:0001217", "UniRef:UniRef50_P47649", "UniRef:UniRef90_P75187" ], "nt": "ATGTGTAGTCGTGATTTGGAACGCATTTGTGATTATGCCAATAACCTCACTAAGTTTGTCAAGCACTACCAGCACTTGGATGTCAGCATCTTTAGTAAGTTAGTTAATTTGCACAAAAGTGTGTTGAACAACTTAAAGCAAACGTTTGCCAGTTTACAAGATAAGGAAAAACCGCTTACTATTCAGTTTGAGAATGTCACTAAAATTCTGACAGAGTTTGAACAGCAGTACCGCGTCGTGTTAACTGAATACTATGACAAGGTTAAAGACGAGAAGCTGAGTGATCGCATCTTCTTAATTGACTTAATTCTGTCGGTGAAACACATTGAACGGATTAATGACTATTGCTACAACATCATTAAGGCATTCCTGTTTGTGAAAAACCCAGAAGTCTTTAACTAG", "aa": "MCSRDLERICDYANNLTKFVKHYQHLDVSIFSKLVNLHKSVLNNLKQTFASLQDKEKPLTIQFENVTKILTEFEQQYRVVLTEYYDKVKDEKLSDRIFLIDLILSVKHIERINDYCYNIIKAFLFVKNPEVFN", "aa_hexdigest": "2ef5fac641a11a628cb67912b411cd79", "start_type": "ATG", "rbs_motif": null, "genes": [ "phoU" ], "truncated": "3-prime", "pseudogene": { "start": 728111, "stop": 728788, "observations": { "mutation": [ 728572 ], "stop": [ 728572 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75187", "query_cov": 1.0, "subject_cov": 0.5911111111111111, "identity": 1.0, "score": 264.0, "evalue": 3.38e-88, "gene_start": 1, "gene_end": 133, "reference_start": 93, "reference_end": 225, "reference_sequence": "MESINYQILKRSEKKLLGLFFDYFQHVIKMHETLNKLLCEADVTKREKLIQAIYEMEDFSNKSEFKLINESIWAISKNSPLTNHLRLTITIIMCSRDLERICDYANNLTKFVKHYQHLDVSIFSKLVNLHKSVLNNLKQTFASLQDKEKPLTIQFENVTKILTEFEQQYRVVLTEYYDKVKDEKLSDRIFLIDLILSVKHIERINDYCYNIIKAFLFVKNPEVFN" }, "paralog": false, "identity": 0.9955555555555555, "score": 443.0, "evalue": 5.26e-161, "description": "Internal stop codon at 728572. Nonsense mutation around 728572.", "psc": { "uniref90_id": "UniRef90_P75187", "gene": "phoU", "product": "phosphate signaling complex protein PhoU", "uniref50_id": "UniRef50_P47649", "cog_id": "COG0704", "cog_category": "P", "go_ids": [ "GO:0006817", "GO:0030643", "GO:0045936" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75187", "UniRef:UniRef50_P47649", "COG:COG0704", "COG:P", "GO:0006817", "GO:0030643", "GO:0045936" ] }, "pscc": { "uniref50_id": "UniRef50_P47649", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47649" ], "product": "Uncharacterized protein MG409" } }, "id": "APKECPOBEN_1284", "locus": "GCA002090215_01284" }, { "type": "cds", "sequence": "contig_1", "start": 728570, "stop": 728788, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAAGCATTAACTACCAAATTTTAAAGCGTTCAGAAAAAAAGCTGTTAGGACTCTTTTTTGATTACTTTCAGCACGTCATTAAGATGCATGAAACACTCAATAAACTGTTATGTGAAGCGGATGTAACTAAGCGCGAAAAGTTGATTCAAGCAATTTACGAAATGGAAGACTTTTCCAATAAATCGGAGTTTAAACTAATTAACGAATCCATTTGA", "aa": "MESINYQILKRSEKKLLGLFFDYFQHVIKMHETLNKLLCEADVTKREKLIQAIYEMEDFSNKSEFKLINESI", "aa_hexdigest": "ef26c005b503b2183c85d4866faf7786", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8674.006699999994, "isoelectric_point": 6.086455726623535 }, "id": "APKECPOBEN_1285", "locus": "GCA002090215_01285" }, { "type": "cds", "sequence": "contig_1", "start": 728791, "stop": 729339, "strand": "-", "frame": 1, "gene": "pstB", "product": "phosphate ABC transporter ATP-binding protein PstB", "db_xrefs": [ "COG:COG1117", "COG:P", "EC:7.3.2.1", "GO:0005524", "GO:0005886", "GO:0006817", "GO:0009898", "GO:0015415", "GO:0016887", "GO:0035435", "GO:0055052", "SO:0001217", "UniRef:UniRef50_P47650", "UniRef:UniRef90_P75186" ], "nt": "TTGGGTAAAAACATTAATTCCGGAATTATTAATGATTTAACCTTGCGCACACGGGTTGGTATGGTGTTCCAGCAACTCACCCCTTTTAACTTTTCCATCTTTGAAAACATTGCCTATGGACTGAGAGCCCATGGCATTCATAACAAGCAAGCGATTCATGAAATTGTGGAACAGGCCTTAAAGTCAACCGCCTTATGGGACGAGGTCAAAGATAACTTACACCGTAATGCCAATACCCTTTCGGGTGGTCAACAACAACGGTTGTGTATTGCCAGAGCCATTGCTCTACAACCCGATGTCTTATTAATGGATGAACCAACTAGTGCGTTGGACTCAATTGCCACTAACTCGATTGAGCTGTTAATCCAGCAATTAAAGGAAAAATACACGATTATTATTGTGACTCACTCAATGGCACAAACTATTAGAATCACTGATGAAACGATTTTCTTTGCTAACGGAAGGGTAGTGGAACAAGGTACTACCAAGCAAATCTTTACGAGACCAAAGCAAAAGGAAACTAACCGTTACATTAGTGGGAGGAATTAA", "aa": "MGKNINSGIINDLTLRTRVGMVFQQLTPFNFSIFENIAYGLRAHGIHNKQAIHEIVEQALKSTALWDEVKDNLHRNANTLSGGQQQRLCIARAIALQPDVLLMDEPTSALDSIATNSIELLIQQLKEKYTIIIVTHSMAQTIRITDETIFFANGRVVEQGTTKQIFTRPKQKETNRYISGRN", "aa_hexdigest": "70ff4345f8e9c529a343819ee6637b4f", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_R5YHY1", "query_cov": 0.9175824175824175, "subject_cov": 0.9881656804733728, "identity": 0.593, "score": 199.0, "evalue": 1.34e-62, "valid": false, "product": "Phosphate ABC transporter ATP-binding protein", "uniref50_id": "UniRef50_A0A836TDX0" }, "genes": [ "pstB" ], "truncated": "3-prime", "pseudogene": { "start": 728791, "stop": 729639, "observations": { "mutation": [ 729360 ], "stop": [ 729360 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75186", "query_cov": 1.0, "subject_cov": 0.5531914893617021, "identity": 0.995, "score": 352.0, "evalue": 1.71e-120, "gene_start": 1, "gene_end": 182, "reference_start": 148, "reference_end": 329, "reference_sequence": "MKKGLKTIWHNFIQKREKVKQYRALYEKQIKQYQQKVAKLDPTTKAEEIANLQSEIDVLQRLIKIKNTKDDVVKQDFDKKNVFEIENLNFWYNKDKQVLFDINLKIKRNKITALIGKSGCGKSTFIRCLNKLNDLNENVRWNGKIFFLGKNINSGIINDLTLRTRVGMVFQQLTPFNFSIFENIAYGLRAHGIHNKQAIHEIVEQALKSTALWDEVKDNLHRNANTLSGGQQQRLCIARAIALQPDVLLMDEPTSALDSIATNSIELLIQQLKEKYTIIIVTHSMAQTIRITDETIFFANGRVVEQGTTKQIFTRPKQKETNRYISGRN" }, "paralog": false, "identity": 0.9964539007092199, "score": 553.0, "evalue": 4.47e-202, "description": "Internal stop codon at 729360. Nonsense mutation around 729360.", "psc": { "uniref90_id": "UniRef90_P75186", "gene": "pstB", "product": "phosphate ABC transporter ATP-binding protein PstB", "ec_ids": [ "7.3.2.1" ], "uniref50_id": "UniRef50_P47650", "cog_id": "COG1117", "cog_category": "P", "go_ids": [ "GO:0005524", "GO:0005886", "GO:0006817", "GO:0009898", "GO:0015415", "GO:0016887", "GO:0035435", "GO:0055052" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75186", "EC:7.3.2.1", "UniRef:UniRef50_P47650", "COG:COG1117", "COG:P", "GO:0005524", "GO:0005886", "GO:0006817", "GO:0009898", "GO:0015415", "GO:0016887", "GO:0035435", "GO:0055052" ] }, "pscc": { "uniref50_id": "UniRef50_P47650", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47650" ], "product": "Phosphate import ATP-binding protein PstB" } }, "id": "APKECPOBEN_1286", "locus": "GCA002090215_01286" }, { "type": "cds", "sequence": "contig_1", "start": 729358, "stop": 729726, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00005.33" ], "nt": "GTGAAGCAGTACCGTGCTTTATATGAAAAACAAATTAAGCAATACCAGCAAAAAGTTGCTAAGTTAGATCCCACTACCAAAGCTGAGGAAATTGCTAACTTGCAGAGTGAAATTGATGTGTTGCAACGCCTCATCAAAATTAAGAATACCAAGGACGATGTTGTCAAACAAGACTTTGACAAGAAGAATGTCTTTGAAATTGAAAACCTTAACTTCTGGTACAACAAGGACAAGCAGGTCTTATTTGACATTAACCTCAAAATTAAGCGCAATAAGATTACCGCTTTAATTGGTAAATCCGGTTGTGGTAAGTCCACCTTTATCCGTTGTTTAAATAAATTAAATGACCTCAATGAAAATGTGCGCTGA", "aa": "MKQYRALYEKQIKQYQQKVAKLDPTTKAEEIANLQSEIDVLQRLIKIKNTKDDVVKQDFDKKNVFEIENLNFWYNKDKQVLFDINLKIKRNKITALIGKSGCGKSTFIRCLNKLNDLNENVR", "aa_hexdigest": "c3d9d7c7bda39285a93dd15c52942bc5", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00005.33", "name": "ABC_tran", "length": 34, "aa_cov": 0.2786885245901639, "hmm_cov": 1.0, "evalue": 3.713635200818957e-11, "score": 39.25088882446289, "start": 82, "stop": 115 } ], "seq_stats": { "molecular_weight": 14423.573699999977, "isoelectric_point": 9.562031364440916 }, "id": "APKECPOBEN_1287", "locus": "GCA002090215_01287" }, { "type": "cds", "sequence": "contig_1", "start": 729807, "stop": 730340, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCCTCGATTATTTACGGATTGTTTGGTCTATCCTTCTTTTTGCGAACACTGCAATTAAGTGCGGGTGGTGCGAATGGTACTAGTTTAATGGCGGGTATTCTTACTATTAGTGTAGTGGTCTTACCCTTTTTAATTCGCACATGTCAGGAAGCACTCAATAATGTCAGTTGGGACTTGCGGGTAAGTGCTTATGCCTTAGGTGTAAGTAAAAGGGAAGTAATTTGGAAAATTGTGTTACCAGGGGCGTTGAAGGGTTTAATTATTGCGCTTATTTTAACGATTAACAGAATTATTGCGGAAACAGCTCCTTTCTTTATTACCGCTGGGTTAGCATCCAGCAACTTGTTTGATTTAAGTCTACCGGGTCAAACCTTAACCACAAGAATTTATGGTCAGTTATTTTCCACTAACAGTACGGCGGTTGATGTAATGCTGGAAACCGCTTTGGTATCAATTGTCTTCCTGATGTTCTTAATCTTTTTAAGCTCTTACTTAATTCCTTACCTGTTTTCGTTTAATAAACAAAAGTGA", "aa": "MPSIIYGLFGLSFFLRTLQLSAGGANGTSLMAGILTISVVVLPFLIRTCQEALNNVSWDLRVSAYALGVSKREVIWKIVLPGALKGLIIALILTINRIIAETAPFFITAGLASSNLFDLSLPGQTLTTRIYGQLFSTNSTAVDVMLETALVSIVFLMFLIFLSSYLIPYLFSFNKQK", "aa_hexdigest": "842b7b2880b4c45e266f286c90844484", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19304.744499999986, "isoelectric_point": 9.461202812194827 }, "id": "APKECPOBEN_1288", "locus": "GCA002090215_01288" }, { "type": "cds", "sequence": "contig_1", "start": 730410, "stop": 730586, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTAGGGATTCTCTTGTTTGTTTTTATTAATGGTAGTCAAGCGATTCAGCGCAGTGGTTCCACTGTCTTTTCGTTTGGTGTAGATACTACAGGGCGAGCTTTAGTCAATACCTTGGTGATTATTCTGGTTGCCATTGGTATTACCTTTCCAATCGCTTTGTTAATTGCCATTTGA", "aa": "MLGILLFVFINGSQAIQRSGSTVFSFGVDTTGRALVNTLVIILVAIGITFPIALLIAI", "aa_hexdigest": "f0a2977fc6b1f3b97b37e326e24a2fff", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6077.2666999999965, "isoelectric_point": 9.3493501663208 }, "id": "APKECPOBEN_1289", "locus": "GCA002090215_01289" }, { "type": "cds", "sequence": "contig_1", "start": 730632, "stop": 730790, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTGGTTTATTCCTAATAACATTGGCGCTCTTTTTACTGATTTAACTGCTAGAAGACAACAGGTTAAAAAGATTACAGCTGCTAATGTTGAGCAGCGCGCTACCTTCTTCAAGGAAAGGATGCAGACTAACCACTTAAATAAGGTTTACACATCGTGA", "aa": "MWFIPNNIGALFTDLTARRQQVKKITAANVEQRATFFKERMQTNHLNKVYTS", "aa_hexdigest": "241db1da5e0dfd144bc01120f48985f2", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6128.9974, "isoelectric_point": 10.533698844909669 }, "id": "APKECPOBEN_1290", "locus": "GCA002090215_01290" }, { "type": "cds", "sequence": "contig_1", "start": 730860, "stop": 731720, "strand": "-", "frame": 2, "gene": "pstA", "product": "phosphate ABC transporter permease PstA", "db_xrefs": [ "COG:COG0581", "COG:P", "GO:0005315", "GO:0005886", "GO:0006817", "GO:0015415", "GO:0035435", "GO:0055052", "SO:0001217", "UniRef:UniRef50_P75185", "UniRef:UniRef90_P75185" ], "nt": "GTGAAACAGAAAATTAAAAGCCGTTTAAAAAAAGACAACTGGCTGCGGTACTTATCGCAAACAGTGGCTGTTTGTTTTTTACTGCTCTTCATTAGCTTCTTTATTTTCCTCTTAATTGAAGCAGCTAAAACTGGTCCTGACTTTACCAAGTCCTTACTGGGTTTGGAATTTAACTTGGGTGCTAAAAAAGCGAGCATCTGGTTTCCGCTGTTAGTTAGTTTTGTGGTTTCGATTGGCTCGTTAATTATTGCGAGCTATATCGGTGTTCGCACTTCGATTTTCTTGGTTTATCGTTGCAAACCCCGGATTCGCAAAAAACTACTTTTAGTAATTGACATTCTGTCCGGGATCCCTTCAGTGATCTTTGGATTGTTTGCTACCCAAATTCTCAGCAGTATCTTCCGTGACGTGTTGCACTTGCCACCCTTATCACTGCTTAACGTGATTGTGATGCTGTCCTTCATGATCATTCCGATTGTGATTTCCTTAACCACCAATGCCTTACTGCACGTGGAATCTAGCTTAATGACGGTTGCCATTTCACTTGGTGAAAACAAAACCAGTGTGATTTATAAGGTAATTAAAAAGGAAATCAAAGCCCAACTAGTAGTAATCTTGGTATTAGCGTTTGGTCGTGCCATTAGCGAAACGATGGCTGTGAACTTTATTTTGCAGAGTGTTAACTACCAGGAAGTGATTGCTAATGATCGTTTCTTTACTTCAGATCTCAAAACTTTGGGTTCTGTTATCTCCACCTTTATCTTTTCGGAAAACGGCGATGAACAGGTTAGTGGGGTCTTATACACTTTTGGCATTATTATCTTTGTCTTAATTTCCTTCTTGAACTTTTTTGCCATTTGA", "aa": "MKQKIKSRLKKDNWLRYLSQTVAVCFLLLFISFFIFLLIEAAKTGPDFTKSLLGLEFNLGAKKASIWFPLLVSFVVSIGSLIIASYIGVRTSIFLVYRCKPRIRKKLLLVIDILSGIPSVIFGLFATQILSSIFRDVLHLPPLSLLNVIVMLSFMIIPIVISLTTNALLHVESSLMTVAISLGENKTSVIYKVIKKEIKAQLVVILVLAFGRAISETMAVNFILQSVNYQEVIANDRFFTSDLKTLGSVISTFIFSENGDEQVSGVLYTFGIIIFVLISFLNFFAI", "aa_hexdigest": "98c1f0a0e0b0ea8e6e84632324317ae2", "start_type": "GTG", "rbs_motif": null, "genes": [ "pstA" ], "truncated": "5-prime", "pseudogene": { "start": 730557, "stop": 731720, "observations": { "mutation": [ 730634, 730862 ], "stop": [ 730634, 730862 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75185", "query_cov": 1.0, "subject_cov": 0.4393241167434716, "identity": 1.0, "score": 506.0, "evalue": 9.16e-175, "gene_start": 1, "gene_end": 286, "reference_start": 1, "reference_end": 286, "reference_sequence": "MKQKIKSRLKKDNWLRYLSQTVAVCFLLLFISFFIFLLIEAAKTGPDFTKSLLGLEFNLGAKKASIWFPLLVSFVVSIGSLIIASYIGVRTSIFLVYRCKPRIRKKLLLVIDILSGIPSVIFGLFATQILSSIFRDVLHLPPLSLLNVIVMLSFMIIPIVISLTTNALLHVESSLMTVAISLGENKTSVIYKVIKKEIKAQLVVILVLAFGRAISETMAVNFILQSVNYQEVIANDRFFTSDLKTLGSVISTFIFSENGDEQVSGVLYTFGIIIFVLISFLNFFAIWSTRPKTLERYPFLKKISNFIYQVVWFIPNNIGALFTDLTARRQQVKKITAANVEQRATFFKERMQTNHLNKVYTSWKILQEIFCAVLAFGFVLGILLFVFINGSQAIQRSGSTVFSFGVDTTGRALVNTLVIILVAIGITFPIALLIAIWLNEYTKSRIAKNTFSFVIDSLSSMPSIIYGLFGLSFFLRTLQLSAGGANGTSLMAGILTISVVVLPFLIRTCQEALNNVSWDLRVSAYALGVSKREVIWKIVLPGALKGLIIALILTINRIIAETAPFFITAGLASSNLFDLSLPGQTLTTRIYGQLFSTNSTAVDVMLETALVSIVFLMFLIFLSSYLIPYLFSFNKQKWLQIKSKLQLWKKA" }, "paralog": false, "identity": 0.9922480620155039, "score": 728.0, "evalue": 1.81e-264, "description": "Internal stop codon at 730634, 730862. Nonsense mutation around 730634, 730862.", "psc": { "uniref90_id": "UniRef90_P75185", "gene": "pstA", "product": "phosphate ABC transporter permease PstA", "uniref50_id": "UniRef50_P75185", "cog_id": "COG0581", "cog_category": "P", "go_ids": [ "GO:0005315", "GO:0005886", "GO:0006817", "GO:0015415", "GO:0035435", "GO:0055052" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75185", "UniRef:UniRef50_P75185", "COG:COG0581", "COG:P", "GO:0005315", "GO:0005886", "GO:0006817", "GO:0015415", "GO:0035435", "GO:0055052" ] }, "pscc": { "uniref50_id": "UniRef50_P75185", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75185" ], "product": "Phosphate transport system permease protein PstA homolog" } }, "id": "APKECPOBEN_1291", "locus": "GCA002090215_01291" }, { "type": "cds", "sequence": "contig_1", "start": 731720, "stop": 731896, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001FB5478A", "UniRef:UniRef90_UPI001FB5478A" ], "nt": "TTGTTATTTAGCAACAACAGTGAAATTAAGAAAATATACGATGACTTTGGTGTGTTAGAGTTAACGGCTGACGAAAAGAAAAAGATGTTTAAAACAGGTAATGCAGAAATGAGCAACATTGCCAACTTCTGGGTTGATGATTACAGTCTGAACAACCAAACCTTCGGTGCACTCTAG", "aa": "MLFSNNSEIKKIYDDFGVLELTADEKKKMFKTGNAEMSNIANFWVDDYSLNNQTFGAL", "aa_hexdigest": "db872504cdb65525c719520b4b55ed97", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001F234BB1", "query_cov": 1.0, "subject_cov": 0.8529411764705882, "identity": 0.569, "score": 71.2, "evalue": 3.43e-15, "valid": false, "uniref50_id": "UniRef50_UPI001FB5478A" }, "pscc": { "uniref50_id": "UniRef50_UPI001FB5478A", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001FB5478A" ] }, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 731720, "stop": 731965, "observations": { "mutation": [ 731899 ], "stop": [ 731899 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001FB5478A", "query_cov": 1.0, "subject_cov": 0.7160493827160493, "identity": 0.983, "score": 118.0, "evalue": 8.42e-34, "gene_start": 1, "gene_end": 58, "reference_start": 24, "reference_end": 81, "reference_sequence": "MNSVVSLLPKQKTLPSIQRFFNWLLFSNNSEIKKIYDDFGVLELTADEKKKMFKTGNAEMSNIANFWVDDYSLNNQTFGAL" }, "paralog": false, "identity": 0.9753086419753086, "score": 159.0, "evalue": 1.77e-52, "description": "Internal stop codon at 731899. Nonsense mutation around 731899.", "psc": { "uniref90_id": "UniRef90_UPI001FB5478A", "uniref50_id": "UniRef50_UPI001FB5478A", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001FB5478A", "UniRef:UniRef50_UPI001FB5478A" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001FB5478A", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001FB5478A" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 6658.412000000002, "isoelectric_point": 4.563057136535646 }, "id": "APKECPOBEN_1292", "locus": "GCA002090215_01292" }, { "type": "cds", "sequence": "contig_1", "start": 731897, "stop": 732877, "strand": "-", "frame": 3, "gene": "pstS", "product": "putative lipo MG412-like protein", "db_xrefs": [ "COG:COG0226", "COG:P", "GO:0005886", "SO:0001217", "UniRef:UniRef50_P47652", "UniRef:UniRef90_P75184" ], "nt": "ATGCCAGTTTTTCTAAAATTAACGCACACAATTAGAAAAGTGCTAAGAGTTGCAAGACTTTCTAGACTAGCATTACTATCACTAACCGCTGTTATTTTTAGCGGTTGTGCCAATATTAATTTAATTAGTGCTGTTGGTTCTTCTTCGGTACAACCGTTGTTAAGCAAACTCAGTTCGCACTATGTCTTGAACCACAATGACAAGGATAACCTTGTAGAAATTAGTGTCCAAGCGGGTGGCTCTAGTGCTGGGGTGAAAGCAATCACCAAGGGACTAGCTGACATTGGTAATGTCTCGAAAAACACCAAGAGCTATGCTGAGGAAAACAAGCAGTTGTGGATGGACAAAAAGCTCAAAACAATTACACTTGGCAAAGATGCCATTGCTGTTATTTATAAAGCACCATCAGAGTTTAAGGGCAAACTAGTTCTCACTAAAGACAACCTCAACGATCTTTACGATCTGTTCGCTGGTAGCAAAAGCGTTGACATTAATAAGTTTGTCGAAAACGGACAAACCACTAAAAACAGTAATCATAATTTGATAGGCTTCCCCCGTACTGGGGGCGCTTTTGCTTCTGGTACCGCTGAAGCTTTCTTGAAGTTTTCGGGTCTTACACAGACAAAAACTTTAGATAAAGATTCCAAAGAAATTTTGGAAGGTCAACGCAACTATGGCCCCAATGCGCGACCAACTAGTGAAACCAACATTGAGGCCTTTAATACCTTTGTCACAACTTTGCGACAACCCAATTTATACGGCATGGTGTACCTCAGTTTAGGGTTTGTGAATAACAACATGAACCTAATTAAGAGTGAAGGTTTTGAGGTTTTAAAAGTCAAATATGATAATAACGCAGTTACCCCCTCCAGTCAAGCAGTTTCTAGCAACACTTACAAATGGGTACGCCCGTTGAACTCAGTGGTTTCCCTGTTACCAAAACAAAAAACACTGCCAAGTATCCAACGCTTTTTTAACTGA", "aa": "MPVFLKLTHTIRKVLRVARLSRLALLSLTAVIFSGCANINLISAVGSSSVQPLLSKLSSHYVLNHNDKDNLVEISVQAGGSSAGVKAITKGLADIGNVSKNTKSYAEENKQLWMDKKLKTITLGKDAIAVIYKAPSEFKGKLVLTKDNLNDLYDLFAGSKSVDINKFVENGQTTKNSNHNLIGFPRTGGAFASGTAEAFLKFSGLTQTKTLDKDSKEILEGQRNYGPNARPTSETNIEAFNTFVTTLRQPNLYGMVYLSLGFVNNNMNLIKSEGFEVLKVKYDNNAVTPSSQAVSSNTYKWVRPLNSVVSLLPKQKTLPSIQRFFN", "aa_hexdigest": "2d738190940de6080edb27631c107cab", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75184", "query_cov": 1.0, "subject_cov": 0.8467532467532467, "identity": 1.0, "score": 620.0, "evalue": 3.3e-223, "valid": true, "gene": "PstS", "product": "Uncharacterized lipoprotein MG412 homolog", "uniref50_id": "UniRef50_P47652", "cog_id": "COG0226", "cog_category": "P", "go_ids": [ "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47652", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47652" ], "product": "Uncharacterized lipoprotein MG412" }, "genes": [ "pstS" ], "id": "APKECPOBEN_1293", "locus": "GCA002090215_01293" }, { "type": "cds", "sequence": "contig_1", "start": 733119, "stop": 734348, "strand": "-", "frame": 2, "gene": null, "product": "putative MG414-like protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75183", "UniRef:UniRef90_P75183" ], "nt": "TTGACACCACAACAACACGCTTCCAACGAATACACCATTAACTTTTTAAGTTTGGATTTTATTGTTGACGGTTATGAATCAGAAAACTTGCACTTCCAAGTAGTTGATCAACTTTTTGGTGCAGTGAAAAACCTACCAACGATCAACAAAATTCCAGAAGGTTACCAGTACAAATTTAAATATCGCAGTAACTTTAGCAACCAGCAATTTAAAGAAAAGGACTTTAGTGTTCCGTTACACAAAGCGATTAAAAGCTTTAGTGTAGGTGAGTTAAAGCAACGCATGGAAAAACTCAATGATTTTGAAAAGCAACAAATTGTTTTTGTCATTAAAAACAGTTTTGCTGGTGTTGATAAAACCGTGTTAGATGTAGATGCTAACAGCTTAACCTTTGTGGAAGATCCCCGACAATACAAACTTGATTTAGGAAACATGAACGAAAACAAAGGTTTCTTCTATGTGTTAGCCGACATCCACAACAGCAACGAACTGTTTAAGTTAGGTTCCACGATCGATCCCGAAATTATAAAAGGGCAAATATACTTTGTTAAACAAAATCAAATAAAGCCCAACACACGCACTTACTTGTTCAAAATTAATACGCGCAAGCTGTTGCTTGACAAACAAAAGTTGTGGTTTAAACCCCACTTGGAAACCCAAACAGTGAGCTTTATGTTTGATGAATTCGAAATTGGCAAATTAGATATAGAAACATCCAACATCCAATTAATAGATGACTATGAGTTTGAGTTTCAATCAGACTTTAGTTTGCAAGATGAACAAGTGTTATTGGAAAAACTCAACATTGTTTTGAGTCAAATGAACCTCCATGTGGAAACGAAGAATGCTAGGTTGAATCCGGTAAATAAAATGGCTTATTTAACCGTTTTAAAAAACAACCAAAAGTTCAACTTAGAGTTCCATGTTGATCGCTATACTAGTCAGCTCTCCATTCAAGCAATGTTGGGTAGTCAAAGACTTTTTGCAATTAACTATGACTTAAAGCTATCACAAAATAAGCAAATGCTATATCTCATTAATAAAGATGGTCTAGAAAAGTCAGTTTGGTTCAACATTAAAAATGACAGTCAAACTAAGCTAGCACAACAACTAGCTAGATTCTTAAAGGAAAACAACTTCCAATTTAGACAAAAACCAGTCTTTGATTTCCACACCCAAAACAAAGCCTTTGCACTGGAAAAGTTGGACAATAAAGATGACACTGATTAA", "aa": "MTPQQHASNEYTINFLSLDFIVDGYESENLHFQVVDQLFGAVKNLPTINKIPEGYQYKFKYRSNFSNQQFKEKDFSVPLHKAIKSFSVGELKQRMEKLNDFEKQQIVFVIKNSFAGVDKTVLDVDANSLTFVEDPRQYKLDLGNMNENKGFFYVLADIHNSNELFKLGSTIDPEIIKGQIYFVKQNQIKPNTRTYLFKINTRKLLLDKQKLWFKPHLETQTVSFMFDEFEIGKLDIETSNIQLIDDYEFEFQSDFSLQDEQVLLEKLNIVLSQMNLHVETKNARLNPVNKMAYLTVLKNNQKFNLEFHVDRYTSQLSIQAMLGSQRLFAINYDLKLSQNKQMLYLINKDGLEKSVWFNIKNDSQTKLAQQLARFLKENNFQFRQKPVFDFHTQNKAFALEKLDNKDDTD", "aa_hexdigest": "97733399d760c30899855a13381ac721", "start_type": "TTG", "rbs_motif": "AAAA", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 733119, "stop": 734648, "observations": { "mutation": [ 734360 ], "stop": [ 734360 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75183", "query_cov": 1.0, "subject_cov": 0.4102306920762287, "identity": 0.998, "score": 805.0, "evalue": 3.91e-285, "gene_start": 1, "gene_end": 409, "reference_start": 589, "reference_end": 997, "reference_sequence": "MRSYHYLPLLGFVAFPTLLANASLDKNLVDSSLYISNSAHHNQGFTVGIKLKDSYFRKDFNFENPLIYKPNNKTTFGWNYSTLWTQLADGYYDRYNQLLRENNFNWYNNIYYIKDVNLGIESSYTSSLAWNKTFSYPGVRNSFKSDIHDLQYFHFHNDSKSGFGPSRINDPWYPQHDKYVTLSAGRTAEEAKNAGWSIPYNLETQRLFSPVWNRNKDFKWINVFFGFMNRSLSVKSPNINDIVQNAKWIKQGKNGNYFIIFNAYAITQNDGFFEDRPNNIFIATLWNNWWLESTPIYIYRVFWEVELIKTPRVFEQDNVQWNEPVLPKETWVFSIDNKQANAYWSSSNGFKDKVIKDRKIMEAFTRNRQVTQSILNMGKNVVVKPLTDDEEQSLFSNKWDIFKYTPIIPVNAQRFGVDFYVEQHNADLKPPISFSQLSRLWLSQLQFDPNSVVDTLSKTFNEKDLSQKDQLQIMFKKEFTQAIAQISKLNEEIDLATDKATALQKFDSLKSKDSNVNFASLQYLYDLLGMEPTNKEHLIFIRNLPEMLQTIFARAKLLAKVKIGDTLQEITLLKNNVNVFDIKTWEKYLTPQQHASNEYTINFLSLDFIVDGYESENLHFQVVDQLFGAVKNLPTINKIPEGYQYKFKYRSNFSNQQFKEKDFSVPLHKAIKSFSVGELKQRMEKLNDFEKQQIVFVIKNSFAGVDKTVLDVDANSLTFVEDPRQYKLDLGNMNENKGFFYVLADIHNSNELFKLGSTIDPEIIKGQIYFVKQNQIKPNTRTYLFKINTRKLLLDKQKLWFKPHLETQTVSFMFDEFEIGKLDIETSNIQLIDDYEFEFQSDFSLQDEQVLLEKLNIVLSQMNLHVETKNARLNPVNKMAYLTVLKNNQKFNLEFHVDRYTSQLSIQAMLGSQRLFAINYDLKLSQNKQMLYLINKDGLEKSVWFNIKNDSQTKLAQQLARFLKENNFQFRQKPVFDFHTQNKAFALEKLDNKDDTD" }, "paralog": false, "identity": 0.9980353634577603, "score": 1005.0, "evalue": 0.0, "description": "Internal stop codon at 734360. Nonsense mutation around 734360.", "psc": { "uniref90_id": "UniRef90_P75183", "product": "Uncharacterized protein MG414 homolog", "uniref50_id": "UniRef50_P75183", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75183", "UniRef:UniRef50_P75183" ] }, "pscc": { "uniref50_id": "UniRef50_P75183", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75183" ], "product": "Uncharacterized protein MG414 homolog" } }, "id": "APKECPOBEN_1294", "locus": "GCA002090215_01294" }, { "type": "cds", "sequence": "contig_1", "start": 734358, "stop": 734693, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTCAAAAAGGAGTTTACTCAAGCGATTGCCCAAATTAGTAAGTTGAACGAAGAGATTGATTTAGCTACTGATAAAGCAACAGCGCTACAAAAGTTTGACTCTTTAAAGTCCAAGGACAGCAACGTTAATTTTGCTAGCTTGCAGTACCTCTATGACTTATTAGGGATGGAGCCTACCAATAAAGAACACCTTATCTTCATTAGGAACTTACCAGAGATGTTGCAAACTATTTTTGCACGAGCCAAACTTTTAGCTAAAGTCAAAATTGGTGATACCTTGCAAGAAATTACGTTGCTTAAAAACAATGTCAATGTGTTTGATATCAAAACTTGA", "aa": "MFKKEFTQAIAQISKLNEEIDLATDKATALQKFDSLKSKDSNVNFASLQYLYDLLGMEPTNKEHLIFIRNLPEMLQTIFARAKLLAKVKIGDTLQEITLLKNNVNVFDIKT", "aa_hexdigest": "aa69fbe639e214cd7a833e100596a99f", "start_type": "ATG", "rbs_motif": "AAAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12692.638499999983, "isoelectric_point": 7.916540336608887 }, "id": "APKECPOBEN_1295", "locus": "GCA002090215_01295" }, { "type": "cds", "sequence": "contig_1", "start": 734790, "stop": 735032, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAGCCTTTACTAGAAACCGCCAAGTAACCCAATCAATCTTGAATATGGGTAAAAACGTTGTAGTAAAGCCATTAACTGATGATGAGGAACAAAGTTTGTTTAGCAATAAGTGGGACATTTTCAAATACACCCCAATTATCCCAGTAAACGCTCAGCGCTTTGGTGTTGACTTTTATGTGGAACAACATAATGCAGATCTAAAACCACCTATTAGTTTTAGTCAACTTTCTAGGTTGTGA", "aa": "MEAFTRNRQVTQSILNMGKNVVVKPLTDDEEQSLFSNKWDIFKYTPIIPVNAQRFGVDFYVEQHNADLKPPISFSQLSRL", "aa_hexdigest": "898462f945d8a76462aab728de47360b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9302.476099999996, "isoelectric_point": 6.54571475982666 }, "id": "APKECPOBEN_1296", "locus": "GCA002090215_01296" }, { "type": "cds", "sequence": "contig_1", "start": 735525, "stop": 735821, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB33HPZ9", "UniRef:UniRef90_A0AB33HPZ9" ], "nt": "TTGCTTAGAGAAAATAACTTTAATTGGTATAACAATATCTATTACATCAAAGATGTTAACTTAGGCATTGAAAGTAGCTATACTAGCAGTTTGGCATGGAACAAAACTTTTAGCTATCCCGGAGTTAGAAACTCCTTTAAATCAGATATACACGACCTTCAGTATTTCCACTTTCACAATGATAGTAAGTCTGGTTTTGGACCATCTCGCATTAATGACCCGTGGTATCCACAGCATGATAAATATGTGACATTAAGCGCTGGTCGAACTGCCGAAGAAGCTAAAAACGCAGGATGA", "aa": "MLRENNFNWYNNIYYIKDVNLGIESSYTSSLAWNKTFSYPGVRNSFKSDIHDLQYFHFHNDSKSGFGPSRINDPWYPQHDKYVTLSAGRTAEEAKNAG", "aa_hexdigest": "7a72347229b26f1751ab42b255f19204", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 735395, "stop": 736112, "observations": { "insertion": [ 735443 ], "mutation": [ 735527, 735863, 735881 ], "stop": [ 735430, 735527, 735863, 735881 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB33HPZ9", "query_cov": 1.0, "subject_cov": 0.4117647058823529, "identity": 0.99, "score": 207.0, "evalue": 2.76e-66, "gene_start": 1, "gene_end": 98, "reference_start": 98, "reference_end": 195, "reference_sequence": "MRSYHYLPLLGFVAFPTLLANASLDKNLVDSSLYISNSAHHNQGFTVGIKLKDSYFRKDFNFENPLIYKPNNKTTFGWNYSTLWTQLADGYYDRYNQLLRENNFNWYNNIYYIKDVNLGIESSYTSSLAWNKTFSYPGVRNSFKSDIHDLQYFHFHNDSKSGFGPSRINDPWYPQHDKYVTLSAGRTAEEAKNAGWSIPYNLETQRLFSPVWNRNKDFKWINVFSVLWTVPYQLSHLT" }, "paralog": false, "identity": 0.9831932773109243, "score": 483.0, "evalue": 4.4e-177, "description": "Internal stop codon at 735430, 735527, 735863, 735881. Frameshift due to insertion around 735443. Nonsense mutation around 735527, 735863, 735881.", "psc": { "uniref90_id": "UniRef90_A0AB33HPZ9", "uniref50_id": "UniRef50_A0AB33HPZ9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB33HPZ9", "UniRef:UniRef50_A0AB33HPZ9" ] }, "pscc": { "uniref50_id": "UniRef50_A0AB33HPZ9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB33HPZ9" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 11434.36359999999, "isoelectric_point": 6.899025917053222 }, "id": "APKECPOBEN_1297", "locus": "GCA002090215_01297" }, { "type": "cds", "sequence": "contig_1", "start": 735879, "stop": 736112, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCGCTCATATCATTATTTACCTTTATTAGGTTTTGTTGCGTTCCCAACTCTTTTAGCGAACGCGTCGTTGGATAAAAATCTTGTTGATAGTAGTTTGTATATTTCTAACTCAGCACACCACAATCAAGGGTTTACTGTTGGGATCAAATTAAAGGATAGTTATTTCCGTAAAGACTTTAATTTCGAAAATCCTTTAATTTATAAACCAAATAACAAAACTACTTTTGGTTGA", "aa": "MRSYHYLPLLGFVAFPTLLANASLDKNLVDSSLYISNSAHHNQGFTVGIKLKDSYFRKDFNFENPLIYKPNNKTTFG", "aa_hexdigest": "ec624bcf87c076c6f7c13776da001dba", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8801.906699999992, "isoelectric_point": 9.347158241271973 }, "id": "APKECPOBEN_1298", "locus": "GCA002090215_01298" }, { "type": "cds", "sequence": "contig_1", "start": 736227, "stop": 736889, "strand": "-", "frame": 2, "gene": null, "product": "putative MG415-like protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75182", "UniRef:UniRef90_P75182" ], "nt": "TTGCCTAACGTTGCTTTCTTTAACGATTACCAAGTAGATTTGGTTTCTACCTTAACGTTAGCTGATAAGCAACTTTTAATAAGAAGACTGAACCTTGCATTAGTTGGCATGAACTTAATGGTTGATGAACAAGCTACTAACATAGAAACTTTTCCCAAACAAATAAGACTCACTACAAAGTCAATAGATCAATCGTTTGATTTTGATGTTGAATTTAATCCAAAAGCTAGTGAACTTAATGTAATTGTTAAGGTTGACGAACAACCATTATTTAAGTTGGATTATGAACTAGTGATTAAAAATTCAAACACTCTACAACTTGTTAACCGCAACAAGCATTTAGGCATGTATATCTGGTCTGTTGCTGATCAACGATACCAAGCTAAATTGCTCGATACCTTGCAGTTATTTCTTCAAAGTAAGCAGATGTACTTAAAAGAAAAGATTCCGCTTAAACTGCAGGATAATACCGCAACTTTTAAAATCGCCAAACAGGCTCAACCAAATAATGGTGAAAATAAAAACACCAATTTTATTGGTTACTTAGTAATTGCTGCTAGTAGTCTGTTTTTAGTGGTAGTTGCTTTTAGTTTTTACTTTTACAAGCGCAAAAAGAATTTGAAATCGCACAAACAAAAAGCAGTTGTCAAAACGGATAAATAA", "aa": "MPNVAFFNDYQVDLVSTLTLADKQLLIRRLNLALVGMNLMVDEQATNIETFPKQIRLTTKSIDQSFDFDVEFNPKASELNVIVKVDEQPLFKLDYELVIKNSNTLQLVNRNKHLGMYIWSVADQRYQAKLLDTLQLFLQSKQMYLKEKIPLKLQDNTATFKIAKQAQPNNGENKNTNFIGYLVIAASSLFLVVVAFSFYFYKRKKNLKSHKQKAVVKTDK", "aa_hexdigest": "9ad9bf0b1e80b026d35592ba731ba42a", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 736227, "stop": 737189, "observations": { "mutation": [ 736961, 737096 ], "stop": [ 736961, 737096 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75182", "query_cov": 1.0, "subject_cov": 0.6395348837209303, "identity": 0.995, "score": 416.0, "evalue": 1.06e-144, "gene_start": 1, "gene_end": 220, "reference_start": 125, "reference_end": 344, "reference_sequence": "MVFSFQDDTALTVALNSIKLVKNPEELIPGLIPTNPKTGIFYIAAEITNSNNFFKWGTDSNREIIKGQMYFLKKETKIPTIRTYLFQMRTHKIALNANDVWFQANNDKVKVIVDGIELDQFDPKLPNVAFFNDYQVDLVSTLTLADKQLLIRRLNLALVGMNLMVDEQATNIETFPKQIRLTTKSIDQSFDFDVEFNPKASELNVIVKVDEQPLFKLDYELVIKNSNTLQLVNRNKHLGMYIWSVADQRYQAKLLDTLQLFLQSKQMYLKEKIPLKLQDNTATFKIAKQAQPNNGENKNTNFIGYLVIAASSLFLVVVAFSFYFYKRKKNLKSHKQKAVVKTDK" }, "paralog": false, "identity": 0.99375, "score": 622.0, "evalue": 1.9e-228, "description": "Internal stop codon at 736961, 737096. Nonsense mutation around 736961, 737096.", "psc": { "uniref90_id": "UniRef90_P75182", "product": "Uncharacterized protein MG415 homolog", "uniref50_id": "UniRef50_P75182", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75182", "UniRef:UniRef50_P75182" ] }, "pscc": { "uniref50_id": "UniRef50_P75182", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75182" ], "product": "Uncharacterized protein MG415 homolog" } }, "id": "APKECPOBEN_1299", "locus": "GCA002090215_01299" }, { "type": "cds", "sequence": "contig_1", "start": 736959, "stop": 737057, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTACTTTCTCAAAAAAGAAACAAAGATTCCAACAATTCGTACTTACTTGTTTCAAATGAGAACACACAAAATTGCTTTGAATGCCAATGACGTTTGA", "aa": "MYFLKKETKIPTIRTYLFQMRTHKIALNANDV", "aa_hexdigest": "f55ad7917052de9d645a5a467e9e5e81", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3885.6002000000008, "isoelectric_point": 9.99467868804932 }, "id": "APKECPOBEN_1300", "locus": "GCA002090215_01300" }, { "type": "cds", "sequence": "contig_1", "start": 737094, "stop": 737261, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTTTTTCATTTCAGGATGATACGGCATTAACTGTTGCCTTAAATTCAATCAAATTAGTTAAAAATCCTGAAGAACTAATTCCTGGTTTAATTCCGACAAATCCTAAAACGGGCATCTTCTATATTGCAGCAGAAATTACTAACAGCAATAATTTCTTTAAATGA", "aa": "MVFSFQDDTALTVALNSIKLVKNPEELIPGLIPTNPKTGIFYIAAEITNSNNFFK", "aa_hexdigest": "7d2cfff18e9a15ac49bde0fbe0e257d7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6086.957300000002, "isoelectric_point": 4.93575038909912 }, "id": "APKECPOBEN_1301", "locus": "GCA002090215_01301" }, { "type": "cds", "sequence": "contig_1", "start": 737239, "stop": 737487, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTTTCTTTATTTGAATCTGCTAAAAATAAACCAACCATTCAAAAAACAAACCAGGGCATTCAATACCGTTTAAGGTTTCGCTCAAATTACAACGAACAGTATTTAAATAAATATCGTTTTAGCATTCCCGTCGTTAATAACGGCAAGGAGTTTAGCGTTTTAGACATCCAAGATAAAGAATTGACTGAAGAGCAGAAAAACCAAATAGCTTTTGTAATTAAAAATGGTTTTTTCATTTCAGGATGA", "aa": "MFSLFESAKNKPTIQKTNQGIQYRLRFRSNYNEQYLNKYRFSIPVVNNGKEFSVLDIQDKELTEEQKNQIAFVIKNGFFISG", "aa_hexdigest": "93c50c456b6b8dda0a806a03bc835af7", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9702.884399999997, "isoelectric_point": 9.520900535583493 }, "id": "APKECPOBEN_1302", "locus": "GCA002090215_01302" }, { "type": "cds", "sequence": "contig_1", "start": 737506, "stop": 737667, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGTGGGCAATTAAGAAAGATTACTTTAGTTAAAAACAACAATAACGTTTTTAATCTAGGCCAGTTTGAACAATTCTTAAATTTAGATCAAATTAGCATTAATTTATTTGAAGTTGAATTTCTCTCTTTTGATTTCATATCAGATCAATATCCTAGTTGA", "aa": "MGGQLRKITLVKNNNNVFNLGQFEQFLNLDQISINLFEVEFLSFDFISDQYPS", "aa_hexdigest": "f92003b0e74c2a2f68530ec69285d987", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6186.908599999997, "isoelectric_point": 4.295857048034668 }, "id": "APKECPOBEN_1303", "locus": "GCA002090215_01303" }, { "type": "cds", "sequence": "contig_1", "start": 737800, "stop": 738174, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F17BD43", "UniRef:UniRef90_UPI001F17BD43" ], "nt": "ATGGGTTTTTCATTAACCCCACTGTTGCTCTCTAGCGCAACTATCAATTCACATGTAATTGACAGCTCGTTTTACTTAACAAAAAATTTAGTAGAGAGCGAAAATCAATCACCTATTCAGTTCAATCAGCTATCGCGAATTATTTTAGTTCAATTAGAGTTTGACCCTGATTCAATAGTTGATAATACTTCTATCACCTTTAATAACCGAACGCTTGGCCAAAAAGCTCTTTTGAAATTACGTTTTAAAAGGGAATTTACCTTAGCGATTCAAGAAATTACAGAGCTTAATCAGTTAGTTGATCAAGCAATTGATAAAAATACAGTTTTACAAAATTTCTTAACGCTTAAAAATGTTGAACGAAAACAGCAATGA", "aa": "MGFSLTPLLLSSATINSHVIDSSFYLTKNLVESENQSPIQFNQLSRIILVQLEFDPDSIVDNTSITFNNRTLGQKALLKLRFKREFTLAIQEITELNQLVDQAIDKNTVLQNFLTLKNVERKQQ", "aa_hexdigest": "19607bc6a64832d6d7eace8082e8d799", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 737506, "stop": 738219, "observations": { "mutation": [ 737802, 738183 ], "stop": [ 737802, 738183 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001F17BD43", "query_cov": 1.0, "subject_cov": 0.5232067510548524, "identity": 1.0, "score": 234.0, "evalue": 1.8e-76, "gene_start": 1, "gene_end": 124, "reference_start": 16, "reference_end": 139, "reference_sequence": "MNVKIAMPFLKYWKLMGFSLTPLLLSSATINSHVIDSSFYLTKNLVESENQSPIQFNQLSRIILVQLEFDPDSIVDNTSITFNNRTLGQKALLKLRFKREFTLAIQEITELNQLVDQAIDKNTVLQNFLTLKNVERKQQWERLSYLYKLLNFDFRDPQELSLVRDLPRLLKTIFESASINFQVRMGGQLRKITLVKNNNNVFNLGQFEQFLNLDQISINLFEFEFLSFDFISDQYPS" }, "paralog": false, "identity": 0.9831223628691983, "score": 448.0, "evalue": 6.02e-163, "description": "Internal stop codon at 737802, 738183. Nonsense mutation around 737802, 738183.", "psc": { "uniref90_id": "UniRef90_UPI001F17BD43", "uniref50_id": "UniRef50_UPI001F17BD43", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001F17BD43", "UniRef:UniRef50_UPI001F17BD43" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001F17BD43", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F17BD43" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 14198.057499999977, "isoelectric_point": 5.770260429382325 }, "id": "APKECPOBEN_1304", "locus": "GCA002090215_01304" }, { "type": "cds", "sequence": "contig_1", "start": 738287, "stop": 738484, "strand": "-", "frame": 3, "gene": "hsdS", "product": "Putative type I specificity subunit S-MpnORF615P", "db_xrefs": [ "COG:COG0732", "COG:V", "EC:3.1.21.3", "GO:0003677", "GO:0009307", "KEGG:K01154", "SO:0001217", "UniParc:UPI0001DD6453", "UniRef:UniRef100_A0AB38W6H7", "UniRef:UniRef50_P75435", "UniRef:UniRef90_P75180" ], "nt": "ATGGCTGAAATTGAACTCTCTTTTCCACCTCTAGAAATTCAAGAAAAGATTGCTGATATTCTCTTTGCCTTTGAAAAACTTTGCAATGATTTAGTAGAAGGTATTCCTGCTGAAATCGAAATGCGCAAAAAGCAATTGGATTATTACTACCATTTAATCTTTAGCAAGATTGCTCACTTTTCCAAACAATTAGCTTAG", "aa": "MAEIELSFPPLEIQEKIADILFAFEKLCNDLVEGIPAEIEMRKKQLDYYYHLIFSKIAHFSKQLA", "aa_hexdigest": "977700c6647ca33d66291832bb7844ef", "start_type": "ATG", "rbs_motif": "AAAA", "ups": { "uniparc_id": "UPI0001DD6453", "uniref100_id": "UniRef100_A0AB38W6H7", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD6453", "UniRef:UniRef100_A0AB38W6H7" ] }, "ips": { "uniref100_id": "UniRef100_A0AB38W6H7", "uniref90_id": "UniRef90_P75180" }, "psc": { "uniref90_id": "UniRef90_P75180", "gene": "HsdS", "product": "Putative type I specificity subunit S.MpnORF615P", "ec_ids": [ "3.1.21.3" ], "uniref50_id": "UniRef50_P75435", "cog_id": "COG0732", "cog_category": "V", "kegg_orthology_id": "K01154", "go_ids": [ "GO:0003677", "GO:0009307" ] }, "pscc": { "uniref50_id": "UniRef50_P75435", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75435" ], "product": "Type I restriction enzyme MpnII specificity subunit" }, "genes": [ "hsdS" ], "id": "APKECPOBEN_1305", "locus": "GCA002090215_01305" }, { "type": "cds", "sequence": "contig_1", "start": 738671, "stop": 739036, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB33HM83", "UniRef:UniRef90_A0AB33HM83", "PFAM:PF01420.25" ], "nt": "ATGCAAGGAATTTTAGCAGAAATTGAGCTAGATTTCCCACCTTTGCAAATTCAAGAGAAAATTGCTACTATCTTAGATACTTTTACCGAGCTAAGCGCCGAGCTAAGAGAGCGAAAGAAGCAGTATGCCTTCTATCGCGATTATCTGCTCAACCAAGAAAATATCCGAAAAATTTACGGAGCAAATATACCTTTCGAAACTTTTCAAGTAAAGGATATTTGTGAAATTAGAAGAGGTCGAGCGATCACTAAAGCGTATATTAGGAATAATCCTGGAGAAAATCCAGTTTATTCCGCAGCTACCACAAATGATGGTGAGTTAGGACGCATTAAAGATTGCGACTTTGATGGTGAATACATCACTTGA", "aa": "MQGILAEIELDFPPLQIQEKIATILDTFTELSAELRERKKQYAFYRDYLLNQENIRKIYGANIPFETFQVKDICEIRRGRAITKAYIRNNPGENPVYSAATTNDGELGRIKDCDFDGEYIT", "aa_hexdigest": "f11f8a4cdb6656ff93304f1aa4eb0818", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 738368, "stop": 738994, "observations": { "mutation": [ 738673 ], "stop": [ 738673 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB33HM83", "query_cov": 0.9173553719008265, "subject_cov": 0.45491803278688525, "identity": 0.946, "score": 210.0, "evalue": 1.06e-66, "gene_start": 15, "gene_end": 121, "reference_start": 1, "reference_end": 111, "reference_sequence": "MQYQEKIATILDTFTELSAELSAELRERKKQYAFYRDYLLNQENIRKIYGANIPFETFQVKDICEIRRGRAITKAYIRNNPGENPVYSAATTNDGELGRIKDCDFDGEYITWTTNGYAGVVFYRNGKFNASQDCGVLKVQNKKICTKFLSFLLKIEAPKFVHNLASRPKLSQKVMAEIELSFPPLEIQEKIADILFAFEKLCNDLVEGIPAEIELRKKQLDYYQNFLFNWVQEQKKNSLSTNLN" }, "paralog": false, "identity": 0.9622641509433962, "score": 405.0, "evalue": 7.72e-146, "description": "Internal stop codon at 738673. Nonsense mutation around 738673.", "psc": { "uniref90_id": "UniRef90_A0AB33HM83", "uniref50_id": "UniRef50_A0AB33HM83", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB33HM83", "UniRef:UniRef50_A0AB33HM83" ] }, "pscc": { "uniref50_id": "UniRef50_A0AB33HM83", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB33HM83" ] } }, "hypothetical": true, "pfams": [ { "id": "PF01420.25", "name": "Methylase_S", "length": 62, "aa_cov": 0.4793388429752066, "hmm_cov": 0.9838709677419355, "evalue": 1.212103427359693e-18, "score": 63.32994079589844, "start": 64, "stop": 121 } ], "seq_stats": { "molecular_weight": 14029.699099999978, "isoelectric_point": 4.922791099548339 }, "id": "APKECPOBEN_1306", "locus": "GCA002090215_01306" }, { "type": "cds", "sequence": "contig_1", "start": 739214, "stop": 739393, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01420.25" ], "nt": "GTGTTGATTAAACGTTTCAAAATCAAAGATATTTGCGATATTAAACGCGGTAGAGTTATCAGCAAACTATATATCAAAAACAATCCAGGAGAATTCCCTGTTTATTCATCAGCAACAGTTAATAACGGTGAAATCGGTAAGATAAAGGATTGTGATTTTAATGGAGAGTACGTCACTTGA", "aa": "MLIKRFKIKDICDIKRGRVISKLYIKNNPGEFPVYSSATVNNGEIGKIKDCDFNGEYVT", "aa_hexdigest": "148aa3d4c075baf84a3b0da6e83ab4d5", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01420.25", "name": "Methylase_S", "length": 60, "aa_cov": 0.9491525423728814, "hmm_cov": 0.9833333333333333, "evalue": 4.591804807995799e-11, "score": 38.6718864440918, "start": 4, "stop": 59 } ], "seq_stats": { "molecular_weight": 6740.782700000003, "isoelectric_point": 9.263607215881347 }, "id": "APKECPOBEN_1307", "locus": "GCA002090215_01307" }, { "type": "cds", "sequence": "contig_1", "start": 739466, "stop": 739864, "strand": "-", "frame": 3, "gene": "rpsI", "product": "30S ribosomal protein S9", "db_xrefs": [ "COG:COG0103", "COG:J", "GO:0003723", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022627", "KEGG:K02996", "RefSeq:WP_010874973.1", "SO:0001217", "UniParc:UPI00001351B9", "UniRef:UniRef100_P75179", "UniRef:UniRef50_Q98Q72", "UniRef:UniRef90_P75179" ], "nt": "ATGGAAAAACAATCTTATTATGGACTTGGACGCCGTAAGTCTTCAAGTGCTAAAGTTTATCTAACGCCTACCCAAGACAAGGGTAAGATTACTGTTAACCGTCGTGATCCTAGTGAATACTTTCCTAATAAACTAGTGATCCAAGACATGGAACAACCTTTAGATCTCACTGATCTTAAAAAGAACTTTGATATCAATGTGGTAGTTAAAGGTGGTGGTTTTACCGGACAAGCTGGTGCAATTCGTTTAGGAATTGTTCGCGCGCTTTTACAGTTCAATCCAGAATTGAAAAAGATTCTCAAGTCCAAGAAACTAACTACCCGTGACAAGCGGGTTAAAGAACGGAAGAAGTTCGGTTTATACGGTGCCCGCCGTGCTCCACAATTTACCAAGCGTTAG", "aa": "MEKQSYYGLGRRKSSSAKVYLTPTQDKGKITVNRRDPSEYFPNKLVIQDMEQPLDLTDLKKNFDINVVVKGGGFTGQAGAIRLGIVRALLQFNPELKKILKSKKLTTRDKRVKERKKFGLYGARRAPQFTKR", "aa_hexdigest": "a7919c96a6331410e88cdda464aa3870", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001351B9", "ncbi_nrp_id": "WP_010874973.1", "uniref100_id": "UniRef100_P75179", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001351B9", "RefSeq:WP_010874973.1", "UniRef:UniRef100_P75179" ] }, "ips": { "uniref100_id": "UniRef100_P75179", "uniref90_id": "UniRef90_P75179" }, "psc": { "uniref90_id": "UniRef90_P75179", "gene": "rpsI", "product": "30S ribosomal protein S9", "uniref50_id": "UniRef50_Q98Q72", "cog_id": "COG0103", "cog_category": "J", "kegg_orthology_id": "K02996", "go_ids": [ "GO:0003723", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022627" ] }, "pscc": { "uniref50_id": "UniRef50_Q98Q72", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98Q72" ], "product": "Small ribosomal subunit protein uS9" }, "genes": [ "rpsI" ], "id": "APKECPOBEN_1308", "locus": "GCA002090215_01308" }, { "type": "cds", "sequence": "contig_1", "start": 739948, "stop": 740310, "strand": "-", "frame": 1, "gene": "rplM", "product": "50S ribosomal protein L13", "db_xrefs": [ "COG:COG0102", "COG:J", "GO:0003729", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0017148", "GO:0022625", "KEGG:K02871", "SO:0001217", "UniRef:UniRef50_P47657", "UniRef:UniRef90_P75178" ], "nt": "ATGCAAAAAACATCAATGCTAACAAAAGAACAGGCCAACAAGCGACGACAGTGGTATATTGTCGACGCTGCTGGGCTCGTTCTCGGTAAGTTAGCGGTTAAAGCGGCCGATCTTATTCGCGGCAAGAATAAAGTTGATTTCACCCCTAACCAAGATTGTGGTGATTACTTGATCATTATTAACAGTGATCAAGTTGTGTTGACTGGCAATAAAAAAGAAAACGAGTTTTGGTACCACCACTCCCAATACATTGGTGGCATTAAGAAGGTAAGTGGTCGTGATATGTTGAAAAAACAATCCGACAAACTGGTCTATAATGCTGTTAAGGGCATGTTACCTGATAACCGTTTAAGCCGTCGTTGA", "aa": "MQKTSMLTKEQANKRRQWYIVDAAGLVLGKLAVKAADLIRGKNKVDFTPNQDCGDYLIIINSDQVVLTGNKKENEFWYHHSQYIGGIKKVSGRDMLKKQSDKLVYNAVKGMLPDNRLSRR", "aa_hexdigest": "2cd7e8a41898bbd90dc239a949ba520f", "start_type": "ATG", "rbs_motif": "AATAA", "psc": { "uniref90_id": "UniRef90_P75178", "query_cov": 1.0, "subject_cov": 0.821917808219178, "identity": 1.0, "score": 243.0, "evalue": 3.32e-81, "valid": true, "gene": "rplM", "product": "50S ribosomal protein L13", "uniref50_id": "UniRef50_P47657", "cog_id": "COG0102", "cog_category": "J", "kegg_orthology_id": "K02871", "go_ids": [ "GO:0003729", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0017148", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_P47657", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47657" ], "product": "Large ribosomal subunit protein uL13" }, "genes": [ "rplM" ], "id": "APKECPOBEN_1309", "locus": "GCA002090215_01309" }, { "type": "cds", "sequence": "contig_1", "start": 740341, "stop": 740562, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTAGAAATTTTTCAACAAAACCTGTTCTTGAGTTCCTTTACTTTGAGTGAATATGAAACTAAGGTACTAGCTAAAGTGGAACAACTAACGCATAAACCCCAAGTGTTGCAATTACAACAGTTAGAACAGTTAAGTAGTACCGTTGTTAAAAAAGCTCAAAAAACAGCGGCACAGGAGATCGCTGATACGTTCTTCAAAGGGCTTTATGAAGAAAAGTAA", "aa": "MLEIFQQNLFLSSFTLSEYETKVLAKVEQLTHKPQVLQLQQLEQLSSTVVKKAQKTAAQEIADTFFKGLYEEK", "aa_hexdigest": "260cfc62722e2f3367007752bcb7a6c8", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8435.6081, "isoelectric_point": 5.7392263412475595 }, "id": "APKECPOBEN_1310", "locus": "GCA002090215_01310" }, { "type": "cds", "sequence": "contig_1", "start": 740599, "stop": 742386, "strand": "-", "frame": 1, "gene": "dnaX", "product": "DNA polymerase III subunit gamma/tau", "db_xrefs": [ "COG:COG2812", "COG:L", "EC:2.7.7.7", "GO:0003887", "GO:0005524", "GO:0006260", "GO:0006261", "GO:0009360", "GO:0016887", "GO:0046872", "KEGG:K02343", "SO:0001217", "UniRef:UniRef50_P47658", "UniRef:UniRef90_P75177" ], "nt": "ATGCGCAAGGTCCTTTACCAGAAATACCGTCCCACCAAGTTTAGCGATACTGTCGGTCAGGACAGCATTAAACGCATTATTGTTAATGCCATTACCCAAGACCAATTACCCCACGGTTACATCTTTGCGGGGGAGCGGGGTACGGGAAAAACGACCTTTGCCAAGATTATTGCCAAAGCCATTAACTGTTTAAACTGGAACGGCGATGTTTGCAACCAGTGTGAGGCATGCCAAGCGATTAACAGCAACAGTGCGATTGATGTGTTTGAAATTGATGCTGCTTCCAAAAACGGGATTAACGACATTAGGGAGCTAGCGGAAAATGTCTTTAACCTACCCTTTAAGTTCAAAAAGAAGGTTTACATTTTGGATGAAGCCCACATGCTCACCCCCCAATCTTGGAGTGGGCTTTTAAAGACCTTAGAAGAAGCACCCGATTATGTCTTGTTTATTTTTGCCACCACCGAATTTAACAAAATTCCGATCACGATCTTGTCGCGTTGCCAAAGCTTCTTCTTTAAACAAATTACTAACGATCTCATCCAGCAACGGTTAGCGGAAGTAGCGGCTAAGGAAAGCATTAAGATTACTACTGATGCTTTGGTCAAACTAGCTGATTTAGCGCAAGGTTCGTTGCGTGATGGGTTGAGCTTGTTGGACCAAATTAGTAACTTTAGTGAAAGTAAAACCATTAGTTTAGCTGATGTTGAAAAGACCTTTAACCTGCTTGACAAGGAACAAAAGTTTGGTTTTATTGAAGCAGTTTTAAGTGGTGATCTCAAACAATCCTTTCACTTAATTGACAACTTTGAAAGTCAAGGGATTAACTTTGTTCACTTTCTCAGGGAACTGTTTGCCCTAACAGTTGATTTATATGGTTATGTCAAAACGGGACAAATTGCAGTTGTAAAACCCAGCGACCAAACAATGGCGGCAAAGCTTCGTTTTCACCCCAAACAGTATGCTCTTTTGGTTCAAGCAATTGAAGCTAACACTGGTTATGGTCCTTCACAACTATCCTTAAGTGATCAGATTAAAGCAATTGTGATTCACTATAACAATGCCGTTAGCAAAGAGCCACACATACCCGCTTATACGCCAGTGGTACAAACTCAATCACCAGCAAAAGAACACGTTCCTTCAAAAACTGTAGAAGAGGCCAAGCCCCAACTACCAGCAAAAGAACCTGTTCTTTATAAAGTAATAGAAGAACCTAAAGTTTCATCATTCGTAAAAGATCCTGTTGCTTCTAAACTAATAGAACAACCCCAAACTATAGTGCAACCAGAAGCACAGCAAGAAATTACAGAAGTAGAACAACCAACAGAAACTTCACCAGCTCCAGCAACTGATTTATTTGGGTTAGCGATTAAACCAGAGGTGGTACGCAAACGCGGTCGTAGACCTCTTTCTGTAGAAAACACTGATTTTTTTCAGCCCGCTGTTAAAAAATTAATACAGCCTGTAAGCAAGCCTGCTCCTATTAAGTTAATAGAACCATCAGATAATAACCCAGTTTCCATCCCAATAAAACTTAACCGGGCTGTGATTGCCGTTTCTGTTTATGGGCACAATGATCCTAAACTAGTAGCCCACTTCCAACAGTTATTGGACAGCTTTAAAAGGGAGTTCACCCAAGCAGAAAAAACTAAAGACAGCAGTTACTTAAAGCAGTTTTCCGATAAGTTTACCGCTAGTGATTTAAGTAAAGTTATTAAGGTTCTAGCAGCATCACCCTTTGGTTTAGTCTTAATTTTCGAAGATAAAGAGATAGCAACAAGGTTGTGA", "aa": "MRKVLYQKYRPTKFSDTVGQDSIKRIIVNAITQDQLPHGYIFAGERGTGKTTFAKIIAKAINCLNWNGDVCNQCEACQAINSNSAIDVFEIDAASKNGINDIRELAENVFNLPFKFKKKVYILDEAHMLTPQSWSGLLKTLEEAPDYVLFIFATTEFNKIPITILSRCQSFFFKQITNDLIQQRLAEVAAKESIKITTDALVKLADLAQGSLRDGLSLLDQISNFSESKTISLADVEKTFNLLDKEQKFGFIEAVLSGDLKQSFHLIDNFESQGINFVHFLRELFALTVDLYGYVKTGQIAVVKPSDQTMAAKLRFHPKQYALLVQAIEANTGYGPSQLSLSDQIKAIVIHYNNAVSKEPHIPAYTPVVQTQSPAKEHVPSKTVEEAKPQLPAKEPVLYKVIEEPKVSSFVKDPVASKLIEQPQTIVQPEAQQEITEVEQPTETSPAPATDLFGLAIKPEVVRKRGRRPLSVENTDFFQPAVKKLIQPVSKPAPIKLIEPSDNNPVSIPIKLNRAVIAVSVYGHNDPKLVAHFQQLLDSFKREFTQAEKTKDSSYLKQFSDKFTASDLSKVIKVLAASPFGLVLIFEDKEIATRL", "aa_hexdigest": "cbb93b6265eb90d308ea0905f9323ac3", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75177", "query_cov": 1.0, "subject_cov": 0.8737151248164464, "identity": 1.0, "score": 1045.0, "evalue": 0.0, "valid": true, "gene": "dnaX", "product": "DNA polymerase III subunit gamma/tau", "ec_ids": [ "2.7.7.7" ], "uniref50_id": "UniRef50_P47658", "cog_id": "COG2812", "cog_category": "L", "kegg_orthology_id": "K02343", "go_ids": [ "GO:0003887", "GO:0005524", "GO:0006260", "GO:0006261", "GO:0009360", "GO:0016887", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_P47658", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47658" ], "product": "DNA polymerase III subunit gamma/tau" }, "genes": [ "dnaX" ], "id": "APKECPOBEN_1311", "locus": "GCA002090215_01311" }, { "type": "cds", "sequence": "contig_1", "start": 742395, "stop": 744251, "strand": "-", "frame": 2, "gene": null, "product": "UvrABC system protein A", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PGE6" ], "nt": "TTGTCTTTAATTAACCACTATCAAATTCCCCTAGACCAACCCTTAGGTCAAATGGATACTGATTTAGTACGGATGATCTTGGAGGGTAGTGATGAACCAATTGAGATTAAAACAGTATCAAACTCAGGTGCCAAAAATGTGCGCTTTGAACACTATGAGGGTGTGGCTCACTTAATTAAACGCCGTCACTTAGAGACTAGTAGTCAAGCCAGTCGTGAATGGTACTCGGCTTACATGTCTGAAATTACCTGTAAGAAGTGCCAGGGCAAGAAGCTTACTACTAATTCTTTAAGTGTCAAGTTAGGTGGGTTGGACATTATTAGCTTTACGGAACTCTCGATTGATAAAGCAATTGAATTCTTGTTGCAAATAGAACTGAATCAAGAACAGAAGAAGATTGGTGAGTTAGCTCTTAAAGAGATTATTAACCGTTTATCCTTCTTGAAGAATGTGGGCTTAGAATACCTTAACTTAGCGCGCCGGGCTTCTACCCTGTCAGGTGGTGAAGCACAACGGATTCGCTTAGCCACCCAAATTGGTTCGCAACTCACTGGCGTCCTTTATGTCTTGGATGAACCCTCAATTGGGCTACACCAAAAGGACAATGATCGCTTAATTAACACGATGATGGTAATGCGGGATTTGGGCAACACCTTACTCGTGGTAGAACACGACAGTGAAACCATGCTAGCAGCTGATTACCTCATTGATATTGGTCCTAAAGCGGGTAACCAAGGTGGGGAAGTAGTAGCTGCTGGGACACCGCTAGAGGTAATGGAAAATCCTGACTCCTTAACGGGACAGTATCTCAGTGGCAAAAAGCAAATTGAGGTACCAAAAACCCGGCATGCTGGTAATGGTCGCACCTTAACCTTAAAAGGAGCCAAGGGCAACAACTTAAAGAACATTAATGTCACTATTCCCTTAAATAAATTAGTACTAGTGACCGGGGTATCGGGTTCAGGGAAATCAACCTTAATTAACCAAACCTTAGTACCGATCTTGGAACGCTTGGTGAACTATAAGAATGTTAAACCTGCACCCTATAAGGAAATCATAGGGGTCAACCACATTGATAAGGTGGTAGTGGTGTCGCAAGATCCGATCGGTCGCACTCCCCGCTCTAACCCCGCTACCTATGTCAGTGTATTTGATGACATTAGGGAGTTGTTTGCCAACACTAAGGAAGCCAAGGCTCGGGGTTACACTAACTCGCGCTTTTCCTTTAATGTAGCCGGTGGGCGCTGTGATAAGTGTTTTGGTGATGGGGTCATTCGGATTGAAATGCACTTTTTACCCGATGTTTATGTGACGTGTGAAATGTGTGATGGCAAGAAGTACAATCCCCAAACCCTGGAAGTTAAGTACTTAGGCAAATCGATCTTTGATGTGTTACAGATGTCATGTCAGGAAGCTTATGATTTTTTTAAAGCAATTCCGAATATTGCGAGAAAGTTAAAACTACTCTGTGATGTCGGTCTGGAATACCTCCAGTTAGGTCTTAATGTTACCTTCTTGTCAGGTGGGGAAGCACAACGAATTAAACTAGCCAAGTTTCTCCAGAAAAAGGCAACTGGTAAAACCTTGTACGTCCTGGATGAACCTTCAACGGGACTACACATAGAAGACATTAATAAGCTGTTAACGGTGATCCAACGGATTATTAAAAACGGTGATAGTGTCATTATTATTGAGCACAACCTAGATATTATTAAGATGGCGGACTACATCATTGATTTGGGTCCTGAAGGTGGGGAAAAAGGTGGACAAATTATTGCCCAAGGCACACCAGAACAACTGTTAAATCAGGTCGATAAGTCCTACACTGCCCAGTATCTCGCTAAAATCTTAAAGTAA", "aa": "MSLINHYQIPLDQPLGQMDTDLVRMILEGSDEPIEIKTVSNSGAKNVRFEHYEGVAHLIKRRHLETSSQASREWYSAYMSEITCKKCQGKKLTTNSLSVKLGGLDIISFTELSIDKAIEFLLQIELNQEQKKIGELALKEIINRLSFLKNVGLEYLNLARRASTLSGGEAQRIRLATQIGSQLTGVLYVLDEPSIGLHQKDNDRLINTMMVMRDLGNTLLVVEHDSETMLAADYLIDIGPKAGNQGGEVVAAGTPLEVMENPDSLTGQYLSGKKQIEVPKTRHAGNGRTLTLKGAKGNNLKNINVTIPLNKLVLVTGVSGSGKSTLINQTLVPILERLVNYKNVKPAPYKEIIGVNHIDKVVVVSQDPIGRTPRSNPATYVSVFDDIRELFANTKEAKARGYTNSRFSFNVAGGRCDKCFGDGVIRIEMHFLPDVYVTCEMCDGKKYNPQTLEVKYLGKSIFDVLQMSCQEAYDFFKAIPNIARKLKLLCDVGLEYLQLGLNVTFLSGGEAQRIKLAKFLQKKATGKTLYVLDEPSTGLHIEDINKLLTVIQRIIKNGDSVIIIEHNLDIIKMADYIIDLGPEGGEKGGQIIAQGTPEQLLNQVDKSYTAQYLAKILK", "aa_hexdigest": "e1b7430cd007607beaa6f06597bed8c9", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0PGE6", "query_cov": 0.8187702265372169, "subject_cov": 0.9980276134122288, "identity": 0.708, "score": 719.0, "evalue": 3.77e-255, "valid": false, "gene": "uvrA", "product": "excinuclease ABC subunit UvrA", "uniref50_id": "UniRef50_A0A3B0PGE6", "kegg_orthology_id": "K03701", "go_ids": [ "GO:0003677", "GO:0006289", "GO:0009380", "GO:0016887" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3B0PGE6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PGE6" ], "product": "UvrABC system protein A" }, "genes": [], "id": "APKECPOBEN_1312", "locus": "GCA002090215_01312" }, { "type": "cds", "sequence": "contig_1", "start": 744261, "stop": 744611, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGACCGGATTGTCATTAACCAAGACAAGCAAACCTTGGGTCGGATTGTTGATGCAATTGAAGGTGTGATTAAACTAACTGAGGGTCGGATTGAAGTGTTGTTGGAAGATGGTAAGATACTTAGTTTTAATAAGAACCACGGTTGTGATCAGTGTGGTTTTTCAATTAGTGAACTAGAGCCACGCTTATTCTCCTTTAACTCCCCTTTAGGTTCGTGTGAGTACTGTAAGGGTTTAGGTTTTAGTTATGAACCGGATGTGGAAAAGATTATTCCCAATCCGTTGTTGTCCATCAATGAAGGTGGTATTGACATCTTTAAGAACATAGTGCACGGTACATCACTAGATTGA", "aa": "MDRIVINQDKQTLGRIVDAIEGVIKLTEGRIEVLLEDGKILSFNKNHGCDQCGFSISELEPRLFSFNSPLGSCEYCKGLGFSYEPDVEKIIPNPLLSINEGGIDIFKNIVHGTSLD", "aa_hexdigest": "c864bc54e6c03a8afa32be3527d46d3a", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12827.505099999984, "isoelectric_point": 4.7428388595581055 }, "id": "APKECPOBEN_1313", "locus": "GCA002090215_01313" }, { "type": "cds", "sequence": "contig_1", "start": 744885, "stop": 745241, "strand": "-", "frame": 2, "gene": null, "product": "UvrABC system protein A", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0G0SCP6", "UniRef:UniRef90_A0A0G0SCP6" ], "nt": "TTGAAACCAGAGCTAAAAACTAATGATTTTATTCGCGTTAAGGGCGCGCGTGAAAACAACCTGAAGAATGTAAACATCGATATTCCCAAAAACCAGTTTGTCGTTATTACGGGGTTGTCGGGTTCGGGTAAATCATCACTTGCCTTTAACACCATTTACGCGGAAGGCCGCCGTCGGTATTTGGAATCACTCTCGTCATATGCCAGACAGTTTCTCGGTAACAGTGATAAACCGGATGTAGATTCGATTGAAGGGTTATCCCCAGCCATTTCAATTGACCAAAAAACCACTTCACACAATCCCCGCTCCACTGTGGGAACAGTAACGGAAATTTACGATTACCTCCGTTTACTGTGA", "aa": "MKPELKTNDFIRVKGARENNLKNVNIDIPKNQFVVITGLSGSGKSSLAFNTIYAEGRRRYLESLSSYARQFLGNSDKPDVDSIEGLSPAISIDQKTTSHNPRSTVGTVTEIYDYLRLL", "aa_hexdigest": "3e2ffd1f63e310734d00ad5da4e8ac46", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_X0QG93", "query_cov": 0.940677966101695, "subject_cov": 0.8809523809523809, "identity": 0.82, "score": 178.0, "evalue": 9.2e-56, "valid": false, "product": "UvrABC system protein A", "uniref50_id": "UniRef50_X0QG93" }, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 744582, "stop": 745214, "observations": { "mutation": [ 744887 ], "stop": [ 744887 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A0G0SCP6", "query_cov": 0.923728813559322, "subject_cov": 0.40977443609022557, "identity": 0.835, "score": 186.0, "evalue": 6.48e-57, "gene_start": 10, "gene_end": 118, "reference_start": 4, "reference_end": 112, "reference_sequence": "MEQFIKIRGARQHNLKNVDLDIPKNKLVVLTGVSGSGKSSLAFDTLYAEGQRRYVESLSSYARQFLGIMEKPDVDSIEGLSPAISIDQKTTSHNPRSTVGTVTEIYDYLRLLFARIGHPHCPVCGREISHQTLDQIVDQTMNLIETTAKADKKAWFLVLSPMVVARKGEFSQLFENLKSKGYRQVRIDGFIHDLSEDLILIKTNKHTIEAVTDRVALSADSLKGQPSKAELKNRLADSIQQALNLSEGMLVLAHVQDKEFTQYQSS" }, "paralog": false, "identity": 0.5806451612903226, "score": 247.0, "evalue": 2.39e-83, "description": "Internal stop codon at 744887. Nonsense mutation around 744887.", "psc": { "uniref90_id": "UniRef90_A0A0G0SCP6", "product": "UvrABC system protein A", "uniref50_id": "UniRef50_A0A0G0SCP6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A0G0SCP6", "UniRef:UniRef50_A0A0G0SCP6" ] } }, "id": "APKECPOBEN_1314", "locus": "GCA002090215_01314" }, { "type": "cds", "sequence": "contig_1", "start": 745219, "stop": 745482, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATCTTTAGCGATTTAAATAAAATTAACACCAAGCAACGCTTTAAACTTTACCTGTTATTTAAGCTATTGTTACACCCCCAGTTGGTGTTGGTCGACTCCTTTGTCAACTTTAACAAACACACTTATAACTTTACCCGTGGTTTGTTAATTGCCCACCAAAACCAACAAGGCATTGCATATTTATTTAACGATCCGCACAATAACCTCGTAAAGGATTTTTTCACCCAAACAATTAACTTTGAAACCAGAGCTAAAAACTAA", "aa": "MIFSDLNKINTKQRFKLYLLFKLLLHPQLVLVDSFVNFNKHTYNFTRGLLIAHQNQQGIAYLFNDPHNNLVKDFFTQTINFETRAKN", "aa_hexdigest": "adb64aeb952abc6ab0434b9db13fe4f5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10373.862199999989, "isoelectric_point": 9.820742988586424 }, "id": "APKECPOBEN_1315", "locus": "GCA002090215_01315" }, { "type": "cds", "sequence": "contig_1", "start": 745483, "stop": 745803, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTACAAAAAAGAAATTAAGCAAAAGCAACAGCAAATTAAAGCACTGCTAAAAAATTATGGCACTTTAAATAAGCTGCTCAATACTCAAATTAGTAAAGTCAATAACGTGGTACGCAAAACCTTCTTTGTGGATTCAGAAGAGTGTGATTTAAACCGTTTACAGGCATCGAATAAGCTGCACTTTAATCTCTTAAACGCGATGGTGAATGTCATTAGTTTTTGTCTCAAAAAGTGCCGGCAAAATCCCAAAAAACTAAACCGTACTGCTAACCTCAAAATGCTGTTGGATAACACCTTTAAAAACGGTATTCCGAGCTGA", "aa": "MYKKEIKQKQQQIKALLKNYGTLNKLLNTQISKVNNVVRKTFFVDSEECDLNRLQASNKLHFNLLNAMVNVISFCLKKCRQNPKKLNRTANLKMLLDNTFKNGIPS", "aa_hexdigest": "405d74d65708e6fa8d928a0ccd470747", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12328.422499999982, "isoelectric_point": 10.112978172302242 }, "id": "APKECPOBEN_1316", "locus": "GCA002090215_01316" }, { "type": "cds", "sequence": "contig_1", "start": 745852, "stop": 746001, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAATAAAACAGTTAATAAATACTTCGAAGCCAACTTGCACCACCCTCAAGCTTACTTGTTGTTACCTAATCGTAATGCCACTACTTTAACAGAAGCAAACACCACTACCATAAACCAACTTAACTTAAAAACCCAGCTCAAACAGTGA", "aa": "MNKTVNKYFEANLHHPQAYLLLPNRNATTLTEANTTTINQLNLKTQLKQ", "aa_hexdigest": "079e920e788379f07eb00fa706fca17f", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5654.373200000001, "isoelectric_point": 9.698124122619632 }, "id": "APKECPOBEN_1317", "locus": "GCA002090215_01317" }, { "type": "cds", "sequence": "contig_1", "start": 746212, "stop": 746574, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAAAAGTTAACTAAGCCAATTGTTTTGACTCATGATGCGGTGATTGGTTTTTTAATTAAATCTCGTTTAGCTTTTTTGCAAGAATACGCTAAGGGCTTAAATCGCTGTGAGCAACACAACAAGCTGGTCAATGAGTTAAAGCAAAACGTTCTGTTAAACCAAAACCAGTATAAGGGTGAGGTTAGCCAAAGCTATTCGGTTGTAAACCAAAAGGCGGTTTTTAAGGGTTTTATCCAAACAGTTAAAGCAGCGCTTAACTACACTAAGTTAAAACACACCTTAGATCCGTTTAACCTAATGAATATTGTTCAAGAACGCTGTTTTGAACAACTCTTAACTACTTACGAAAAATTAGATTGA", "aa": "MEKLTKPIVLTHDAVIGFLIKSRLAFLQEYAKGLNRCEQHNKLVNELKQNVLLNQNQYKGEVSQSYSVVNQKAVFKGFIQTVKAALNYTKLKHTLDPFNLMNIVQERCFEQLLTTYEKLD", "aa_hexdigest": "9084ed01dc385e38f2f74d0a8d14c4ac", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13918.025699999986, "isoelectric_point": 9.250649070739744 }, "id": "APKECPOBEN_1318", "locus": "GCA002090215_01318" }, { "type": "cds", "sequence": "contig_1", "start": 746599, "stop": 746733, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCGCTTACTTAATCCCGATTTGTTAGTGAGCACAATTGCTGAAAGTGAGCGCCTAGTAGAACAGTTAATGAGCAACATCCAGCGCTTGTACCAGCAAATTTTGCAGCTAAAACCAGGTCAATCACTTAACTGA", "aa": "MRLLNPDLLVSTIAESERLVEQLMSNIQRLYQQILQLKPGQSLN", "aa_hexdigest": "561e64c6286c3fb9f7101f765077ed75", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5094.9057999999995, "isoelectric_point": 6.014099693298339 }, "id": "APKECPOBEN_1319", "locus": "GCA002090215_01319" }, { "type": "cds", "sequence": "contig_1", "start": 746755, "stop": 747324, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGGCTTTAATGCACTCAAGAAGTTAAGCCAGTTTGTGGTGTTTGAACTAGATAACACCAATAAGTTAACGAGTGAAACACTTAGTCAGTTCTTCACCACTAAGTTTGTCAATAAGGTGTACTTTAACCAGATCCAGTTTGAGTACAACAACTTTGTTGGTTTATTGGAAGACCATGAACGTGACCTAGTGGAATTTAGCACCCACTTCTTTAACAGCTTCTTTGAACAACCTGATCACTTAACTAAAAGTTTTTTTGAACAGTACTATGCGCTTACAAGACAACGAGAACGTTTACTGCACAATTTAAAAGTTCAAAGCTATGAAACCAAACACGGGGTTAATACCTACTTTGAAAATTTGCAAATTACCCGTGCACAACAGCAAGCCAATCAGCTTAAGCGTGCCTTCAAAAAACAGCAGCGCAATTCGGTGCAGCTGATTAACCGCTTTATGTCGTCCTTGTGGTGGAGTCAAACCAAGATTAAGTTCAAGAGTATCTTTAACTTTTACAAAACCCGGATTTTGGAAAAACGGATTGTAGCCAAGCGAATCCAAATCAAAATTTGA", "aa": "MGFNALKKLSQFVVFELDNTNKLTSETLSQFFTTKFVNKVYFNQIQFEYNNFVGLLEDHERDLVEFSTHFFNSFFEQPDHLTKSFFEQYYALTRQRERLLHNLKVQSYETKHGVNTYFENLQITRAQQQANQLKRAFKKQQRNSVQLINRFMSSLWWSQTKIKFKSIFNFYKTRILEKRIVAKRIQIKI", "aa_hexdigest": "eadd1df54e193b03ba5c58100650fccf", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 22985.023500000014, "isoelectric_point": 9.958447456359863 }, "id": "APKECPOBEN_1320", "locus": "GCA002090215_01320" }, { "type": "cds", "sequence": "contig_1", "start": 747784, "stop": 749469, "strand": "-", "frame": 1, "gene": "rnjA", "product": "putative MG423-like protein", "db_xrefs": [ "COG:COG0595", "COG:J", "GO:0003723", "GO:0005886", "GO:0046872", "RefSeq:WP_010874978.1", "SO:0001217", "UniParc:UPI00001397B4", "UniRef:UniRef100_P75174", "UniRef:UniRef50_P47662", "UniRef:UniRef90_P75174" ], "nt": "ATGGCTAAAATTAACTTCTTTGCCTTCGGTGGTCAGGACGAGAGGGGCAAGAATTGTTTTGTTCTCGAAATCAATAACGACGTTTTTATTTTTAACGTTGGTAGTTTGACACCAACAACCGCTGTTTTAGGGGTCAAAAAGATCATCCCTGACTTTAGTTGGATCCAGGAAAACCAGGCCCGCATTAAGGGCATCTTTATCGGTAATCCAGTAACGGAAAACATTGGTTCGCTGGAGTTCTTATTCCACACCGTTGGTTTCTTTCCGATTTACACCAGTACGATTGGCGCTGTGGTTATTAAAACTAAGATCCACGAAAACAAGCTCAATATTCCCCATGACGAGTTGGAAATCCACGAGCTTAAACCGTTAGAAACGGTCAAGATCGGGCACCATAACATTACGCCCTTTAAGGTATCGAGTTCGATTCCTTCCTCGTTTGGCTTTGCGCTCCACACTGATGATGGGTACATTGTCTATGTCGATGACTTCATTGTGCTCAATGATAAGAACATCGCCTTTGAAAACCAACTCAATCAGATTATTCCCCAAGTAGCGAACAAGACCCTTTTGTTAATTACTGGAGTTGGTCTAGTGGGACGTAACACTGGTTTTACAACCCCTAAGCACAAATCCTTGGAACAACTCAACCGCATTATTGCCTCCGCTAAGGGTCGGGTATTTGCTGCTTGTTATGATTCCAACGCTTACAGTGTGATGACCTTGGCCCAAATTGCACGGATGCAAAACCGCCCCTTTGTCATCTACTCCCATTCATTTGTCCACCTCTTCAACGCGATTGTGCGCCAAAAGCTGTTTAACAACACCCACTTAAACACTATTTCGATTGAAGAGATTAACAACTCCACAAACGCCATTGTTGTTTTAACCGCGCCACCAGATAAGTTGTATGCCAAGCTGTTTAAGATTGGTACTAATGAGGATGAACGGGTACGGTATCGCAAGACCGATAGCTTTATCTTCATGATTCCCCGCATAGCTGGGTATGAAGAGTTGGAAGCACAAATCTTGGACGATGTCGCGCGTAATGAGGTGAGTTACTACAACTTGGGTCGGGAAATCTTGTCGATCAATGCATCGGATGAAGACATGAAGTTTTTAGTAACTTCCTTAAAACCTAAGTACATTATTCCCACATCCGGTCTGTACCGTGACTTCATTAACTTTACCATGGTAATGAAGCAAGCTGGTGTGGAACAAAGTCAGGTCTTAATTCCCTTTAATGGTGAAGTCCTAGCCATTAACCACAAGCAAATTGACAACAAAAAACGGGAATTAAAGCTCAACCCTAAGTGTGTTGACTCCGCTGGTTTGCAGGAAATAGGGGCCAGCATCATGTTTGAGCGTGATCAGATGTCTGAAGCGGGTGTGGTTACCATCATCATCTACTATGACTCCAAGAAGAGTGAGTTCCTAAACGAAATTACCTATTCCTTTTTAGGAGTTAGCCTAGATAGTAACAATCAGGTTAAACTCAAGACCAAAATGGAAGAGTTAATTCGCAAACAAATTAACGACATTAAGGACTTTACGACAATTAAGCGACGTTTAGGCAAAGACACATCGAAGGAACTCAAGGTTTCTATTAAGCGAGCTGTCATGAATTTATTTACCAAAATGACTGCTAAAGCACCACTAATCCTGTCAACTATTATTTCTATTTAG", "aa": "MAKINFFAFGGQDERGKNCFVLEINNDVFIFNVGSLTPTTAVLGVKKIIPDFSWIQENQARIKGIFIGNPVTENIGSLEFLFHTVGFFPIYTSTIGAVVIKTKIHENKLNIPHDELEIHELKPLETVKIGHHNITPFKVSSSIPSSFGFALHTDDGYIVYVDDFIVLNDKNIAFENQLNQIIPQVANKTLLLITGVGLVGRNTGFTTPKHKSLEQLNRIIASAKGRVFAACYDSNAYSVMTLAQIARMQNRPFVIYSHSFVHLFNAIVRQKLFNNTHLNTISIEEINNSTNAIVVLTAPPDKLYAKLFKIGTNEDERVRYRKTDSFIFMIPRIAGYEELEAQILDDVARNEVSYYNLGREILSINASDEDMKFLVTSLKPKYIIPTSGLYRDFINFTMVMKQAGVEQSQVLIPFNGEVLAINHKQIDNKKRELKLNPKCVDSAGLQEIGASIMFERDQMSEAGVVTIIIYYDSKKSEFLNEITYSFLGVSLDSNNQVKLKTKMEELIRKQINDIKDFTTIKRRLGKDTSKELKVSIKRAVMNLFTKMTAKAPLILSTIISI", "aa_hexdigest": "d8eed88c141103209b004c9f0e6aed2d", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001397B4", "ncbi_nrp_id": "WP_010874978.1", "uniref100_id": "UniRef100_P75174", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001397B4", "RefSeq:WP_010874978.1", "UniRef:UniRef100_P75174" ] }, "ips": { "uniref100_id": "UniRef100_P75174", "uniref90_id": "UniRef90_P75174" }, "psc": { "uniref90_id": "UniRef90_P75174", "gene": "RnjA", "product": "Uncharacterized protein MG423 homolog", "uniref50_id": "UniRef50_P47662", "cog_id": "COG0595", "cog_category": "J", "go_ids": [ "GO:0005886", "GO:0046872", "GO:0003723" ] }, "pscc": { "uniref50_id": "UniRef50_P47662", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47662" ], "product": "Uncharacterized protein MG423" }, "genes": [ "rnjA" ], "id": "APKECPOBEN_1321", "locus": "GCA002090215_01321" }, { "type": "cds", "sequence": "contig_1", "start": 749474, "stop": 749734, "strand": "-", "frame": 3, "gene": "rpsO", "product": "30S ribosomal protein S15", "db_xrefs": [ "COG:COG0184", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022627", "RefSeq:WP_010874979.1", "SO:0001217", "UniParc:UPI0000134D13", "UniRef:UniRef100_P75173", "UniRef:UniRef50_P47663", "UniRef:UniRef90_P75173" ], "nt": "ATGCAAATTGATAAAAACGGGATCATTAAATCCGCACAACTCCACGACAAGGACGTGGGTAGTATTCAGGTACAAGTGTCCCTTTTAACGAGCCAAATTAAACAGCTCACCGACCACTTACTAGCTAACAAGAAGGACTTTATTTCCAAACGTGGTTTGTACGCGAAAGTATCAAAGCGCAAGCGCTTGTTGAAGTACTTAAAGCACAACGATTTAGAGGCTTACAGAAATTTAGTTAAAACCTTAAATCTCCGGGGTTAG", "aa": "MQIDKNGIIKSAQLHDKDVGSIQVQVSLLTSQIKQLTDHLLANKKDFISKRGLYAKVSKRKRLLKYLKHNDLEAYRNLVKTLNLRG", "aa_hexdigest": "e53f2d5f814b7b149311b178fd0d2b9b", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000134D13", "ncbi_nrp_id": "WP_010874979.1", "uniref100_id": "UniRef100_P75173", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000134D13", "RefSeq:WP_010874979.1", "UniRef:UniRef100_P75173" ] }, "ips": { "uniref100_id": "UniRef100_P75173", "uniref90_id": "UniRef90_P75173" }, "psc": { "uniref90_id": "UniRef90_P75173", "gene": "rpsO", "product": "30S ribosomal protein S15", "uniref50_id": "UniRef50_P47663", "cog_id": "COG0184", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022627" ] }, "pscc": { "uniref50_id": "UniRef50_P47663", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47663" ], "product": "Small ribosomal subunit protein uS15" }, "genes": [ "rpsO" ], "id": "APKECPOBEN_1322", "locus": "GCA002090215_01322" }, { "type": "cds", "sequence": "contig_1", "start": 749892, "stop": 750233, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00270.35" ], "nt": "ATGGACTCAACTTTTAATGAACTAGGCGTCTCTCCTGCCTTAATTGCCACACTCAAAGATAACAACATTAACCAACCAACTACGATTCAACAACTGGCTATTCCCCAGTTTCTCCAACATCAAAACCTGATTGTCCACTCCCCCACCGGCACTGGTAAAACCGCTGTTTTTGCCATTCCGGTCATTGAAACACTCCTTAAAAAACCAAGTAAAGGTACGACCCAAACATTGGTAGTTGCACCTACCCGTGAACTGGCCGAACAGATTAAAACCACCTTTATTAACTTTGCTAAACACACCCATTTAAAGGTAGTGAGCTTAATTGGTGGCATCCCCATTTGA", "aa": "MDSTFNELGVSPALIATLKDNNINQPTTIQQLAIPQFLQHQNLIVHSPTGTGKTAVFAIPVIETLLKKPSKGTTQTLVVAPTRELAEQIKTTFINFAKHTHLKVVSLIGGIPI", "aa_hexdigest": "6f28b63d51c4fb90203bf0d1d38e6b1a", "start_type": "ATG", "rbs_motif": "AATAA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00270.35", "name": "DEAD", "length": 84, "aa_cov": 0.7433628318584071, "hmm_cov": 0.9761904761904762, "evalue": 4.785813010677931e-25, "score": 83.68274688720703, "start": 27, "stop": 110 } ], "seq_stats": { "molecular_weight": 12220.109599999982, "isoelectric_point": 9.523156929016114 }, "id": "APKECPOBEN_1323", "locus": "GCA002090215_01323" }, { "type": "cds", "sequence": "contig_1", "start": 750282, "stop": 751229, "strand": "+", "frame": 3, "gene": "srmB", "product": "putative ATP-dependent RNA helicase MG425-like protein", "db_xrefs": [ "COG:COG0513", "COG:L", "EC:3.6.4.13", "GO:0003689", "GO:0003723", "GO:0003724", "GO:0005524", "GO:0005829", "GO:0016887", "GO:0061775", "GO:0140584", "GO:0140665", "GO:0140849", "SO:0001217", "UniRef:UniRef50_P47664", "UniRef:UniRef90_P75172" ], "nt": "ATGGGGCGGGTGATGGACCTGTTAGAACGGGGTGTAATTAAGTTTGAACACCTAGAACACCTCATTATTGACGAGGTTGACCTAATGCTCGACCGTGGTTTTAAGAGAAAACTGTTTGATCTGTTAAGTCGCATTGAAAAGTTTGAACAAATTGCGGTTTATTCTGCTAGTTACAATGAAGAGACAATTGAAACAGCTAAACAAATTACCAAGAATGGTATTTTCCTAGCAGCACCAGAATTAAAACAAAATGCCCCTGAACCAGATAACAAACTCATTGATCAATTTGTTTGTTATCTTTTTTCTAACCGGAAGAAACAAGCCCTCTATTCTTTGGTATCGCAAACTAGAGCGAAGTCGATTATTGTTTTTTGTGACACCAAGAAGTTAGTTGATGAGCTGTGCATCTTCTTGCGCAAGAATGATGTCAAAACTTATCCACTTCACGGGGACAAAGCGCAGTTCATTCGCGAACGCAACTTAAAGCTGTTTGCTAACACAACTGCTCCTATAGTATTAGTAACAACTGATCTAATCGGTCGTGGTATCCATGTGGAAGGGGTGGACATGGTAGTCAACTATTCCGCTTGTGTAAACTTTGAAACTTATTTGCACCGGATGGGTCGCACAGGACGGAACAACCACAAGGGCTCATGTATTACCTTCTGTACCTCACACGAAAAGCAAGCATTTTTAAAGTTATTAGAACAAGTTAACGACAAGCGCATTAGCCCTCTCCGCCCCATGCGCTTAAGGTTAATTCCCTTGAAGTGCAAAACCCAACCCAAGAAGGGGAAACTTTCCTTACAGTCAGTGCAAAAGATTTACGTCAATCCTCGTTCCAATGGCACCTTTAAACGCGTACCTTTAGCGGGGGATTTGTTTAAAAGCCGGATGCGTCAACCTGAGCGGGACATGCAAAAGAACAAATTGCATGACAGTGATTGA", "aa": "MGRVMDLLERGVIKFEHLEHLIIDEVDLMLDRGFKRKLFDLLSRIEKFEQIAVYSASYNEETIETAKQITKNGIFLAAPELKQNAPEPDNKLIDQFVCYLFSNRKKQALYSLVSQTRAKSIIVFCDTKKLVDELCIFLRKNDVKTYPLHGDKAQFIRERNLKLFANTTAPIVLVTTDLIGRGIHVEGVDMVVNYSACVNFETYLHRMGRTGRNNHKGSCITFCTSHEKQAFLKLLEQVNDKRISPLRPMRLRLIPLKCKTQPKKGKLSLQSVQKIYVNPRSNGTFKRVPLAGDLFKSRMRQPERDMQKNKLHDSD", "aa_hexdigest": "eca2e53d249dafd2789a5b9ba7b535de", "start_type": "ATG", "rbs_motif": null, "genes": [ "srmB" ], "truncated": "both", "pseudogene": { "start": 749982, "stop": 751244, "observations": { "mutation": [ 750231, 751227 ], "stop": [ 750231, 751227 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75172", "query_cov": 1.0, "subject_cov": 0.7, "identity": 1.0, "score": 625.0, "evalue": 4.81e-224, "gene_start": 1, "gene_end": 315, "reference_start": 131, "reference_end": 445, "reference_sequence": "MDSTFNELGVSPALIATLKDNNINQPTTIQQLAIPQFLQHQNLIVHSPTGTGKTAVFGIPVIETLLKKPSKGTTQTLVVAPTRELAEQIKTTFINFAKHTHLKVVSLIGGIPIWQQLKQLENQPEIVVGTMGRVMDLLERGVIKFEHLEHLIIDEVDLMLDRGFKRKLFDLLSRIEKFEQIAVYSASYNEETIETAKQITKNGIFLAAPELKQNAPEPDNKLIDQFVCYLFSNRKKQALYSLVSQTRAKSIIVFCDTKKLVDELCIFLRKNDVKTYPLHGDKAQFIRERNLKLFANTTAPIVLVTTDLIGRGIHVEGVDMVVNYSACVNFETYLHRMGRTGRNNHKGSCITFCTSHEKQAFLKLLEQVNDKRISPLRPMRLRLIPLKCKTQPKKGKLSLQSVQKIYVNPRSNGTFKRVPLAGDLFKSRMRQPERDMQKNKLHDSDWQSNM" }, "paralog": false, "identity": 0.9928571428571429, "score": 824.0, "evalue": 6.63e-305, "description": "Internal stop codon at 750231, 751227. Nonsense mutation around 750231, 751227.", "psc": { "uniref90_id": "UniRef90_P75172", "gene": "SrmB", "product": "Probable ATP-dependent RNA helicase MG425 homolog", "ec_ids": [ "3.6.4.13" ], "uniref50_id": "UniRef50_P47664", "cog_id": "COG0513", "cog_category": "L", "go_ids": [ "GO:0005829", "GO:0005524", "GO:0016887", "GO:0140849", "GO:0140665", "GO:0140584", "GO:0061775", "GO:0003689", "GO:0003723", "GO:0003724" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75172", "EC:3.6.4.13", "UniRef:UniRef50_P47664", "COG:COG0513", "COG:L", "GO:0005829", "GO:0005524", "GO:0016887", "GO:0140849", "GO:0140665", "GO:0140584", "GO:0061775", "GO:0003689", "GO:0003723", "GO:0003724" ] }, "pscc": { "uniref50_id": "UniRef50_P47664", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47664" ], "product": "Probable ATP-dependent RNA helicase MG425" } }, "id": "APKECPOBEN_1324", "locus": "GCA002090215_01324" }, { "type": "cds", "sequence": "contig_1", "start": 751248, "stop": 751463, "strand": "+", "frame": 3, "gene": "rpmB", "product": "50S ribosomal protein L28", "db_xrefs": [ "COG:COG0227", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:1990904", "SO:0001217", "UniRef:UniRef50_Q6MTC5", "UniRef:UniRef90_P75171" ], "nt": "TTGTTACAATTATTTGATATGGCAAAAAAAGACCAACTTACTTTAAGAGGGCCTTTGTATGGCAACAATCGTTCCCACTCCAAGACTATTACGCGCAGAAAGTGGAATGTTAACCTCCAACCATGCAAGGTCAAAACTGCTGATGGCAAGACCACCAGAATCTTAGTTTCTACCAGAACACTGCGCACCTTAAAGAAACACAACCGCCTCTCTTAG", "aa": "MLQLFDMAKKDQLTLRGPLYGNNRSHSKTITRRKWNVNLQPCKVKTADGKTTRILVSTRTLRTLKKHNRLS", "aa_hexdigest": "74510e73d215b5087aac105bb3dc7cc6", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75171", "query_cov": 0.9154929577464789, "subject_cov": 1.0, "identity": 1.0, "score": 127.0, "evalue": 4.1e-37, "valid": true, "gene": "rpmB", "product": "50S ribosomal protein L28", "uniref50_id": "UniRef50_Q6MTC5", "cog_id": "COG0227", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_Q6MTC5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q6MTC5" ], "product": "Large ribosomal subunit protein bL28" }, "genes": [ "rpmB" ], "id": "APKECPOBEN_1325", "locus": "GCA002090215_01325" }, { "type": "cds", "sequence": "contig_1", "start": 751507, "stop": 751932, "strand": "+", "frame": 1, "gene": "yhfA", "product": "Hydroperoxide reductase", "db_xrefs": [ "COG:COG1765", "COG:R", "EC:1.11.1.-", "GO:0004601", "GO:0005737", "RefSeq:WP_010874982.1", "SO:0001217", "UniParc:UPI00001397C2", "UniRef:UniRef100_P75170", "UniRef:UniRef50_P47666", "UniRef:UniRef90_P75170" ], "nt": "ATGGACAAAAAGTACGACATCACTGCTGTTTTAAACGAAGACAGCTCCATGACTGCCATCAGTGATCAGTTTCAAATTACACTCGATGCCCGTCCCAAGCACACCGCTAAGGGTTTTGGTCCTTTAGCTGCTCTGCTCTCTGGTTTAGCTGCTTGTGAATTGGCAACCGCTAACTTGATGGCACCAGCTAAAATGATTACCATCAATAAACTGTTGATGAATGTCACTGGTTCACGCTCAACCAACCCCACCGATGGTTATTTTGGTTTAAGGGAAATTAACCTGCACTGGGAAATCCACTCCCCTAACAGTGAAACGGAAATCAAGGAGTTCATTGACTTTGTTTCCAAACGCTGCCCAGCGCACAATACGCTCCAAGGTGTGAGTCAACTCAAGATTAACGTTAATGTAACTTTAGTGCATTAA", "aa": "MDKKYDITAVLNEDSSMTAISDQFQITLDARPKHTAKGFGPLAALLSGLAACELATANLMAPAKMITINKLLMNVTGSRSTNPTDGYFGLREINLHWEIHSPNSETEIKEFIDFVSKRCPAHNTLQGVSQLKINVNVTLVH", "aa_hexdigest": "72cd36b4ae77fb22d150dbc14598aff0", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001397C2", "ncbi_nrp_id": "WP_010874982.1", "uniref100_id": "UniRef100_P75170", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001397C2", "RefSeq:WP_010874982.1", "UniRef:UniRef100_P75170" ] }, "ips": { "uniref100_id": "UniRef100_P75170", "uniref90_id": "UniRef90_P75170" }, "psc": { "uniref90_id": "UniRef90_P75170", "gene": "YhfA", "product": "Hydroperoxide reductase", "ec_ids": [ "1.11.1.-" ], "uniref50_id": "UniRef50_P47666", "cog_id": "COG1765", "cog_category": "R", "go_ids": [ "GO:0005737", "GO:0004601" ] }, "pscc": { "uniref50_id": "UniRef50_P47666", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47666" ], "product": "Hydroperoxide reductase" }, "genes": [ "yhfA" ], "id": "APKECPOBEN_1326", "locus": "GCA002090215_01326" }, { "type": "cds", "sequence": "contig_1", "start": 752163, "stop": 752309, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTCAATTGCGCTCCACGCCGAGCATGACTCCAAGAAGCGCTATGACCCCAAATACGGTTTGAGCTTTGAGACCTACTTGAAGTTAAATGGTGCTAACTTCATTAAGTCGAGTTACCGTAGCTTAATGAATAAGGTTGATGACTGA", "aa": "MSIALHAEHDSKKRYDPKYGLSFETYLKLNGANFIKSSYRSLMNKVDD", "aa_hexdigest": "27e5f115f6e63c225c5d5c1df04044e8", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5583.270300000002, "isoelectric_point": 9.049830055236818 }, "id": "APKECPOBEN_1327", "locus": "GCA002090215_01327" }, { "type": "cds", "sequence": "contig_1", "start": 752325, "stop": 752585, "strand": "+", "frame": 3, "gene": null, "product": "putative MG428-like protein", "db_xrefs": [ "GO:0003700", "GO:0006352", "SO:0001217", "UniRef:UniRef50_P47667", "UniRef:UniRef90_P75169" ], "nt": "ATGAAAACACTCGAGGAACAAAACACAGTCTCTTACACTCCTGAAAACTATTTAAGAAGTGTAGAATTCCGTGAAATGGTGCACTTAGCGTTTAAGAAAGCCAAGAATGAGGATGAACGTCAGGTGTTTGCGCTTTATGTAAAGGGTTACAAAAATTTAGAGATTGCTAAAAAACTCAACCTAACGACCCGTAGAGTGCGCTACCTAATTTCAATCTTTAAGGATCACATTAAGGTATTGACTAAACGGTATGGTTACTAG", "aa": "MKTLEEQNTVSYTPENYLRSVEFREMVHLAFKKAKNEDERQVFALYVKGYKNLEIAKKLNLTTRRVRYLISIFKDHIKVLTKRYGY", "aa_hexdigest": "239f6dca832476888c6b6e2744552adc", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 752067, "stop": 752585, "observations": { "mutation": [ 752121, 752307 ], "stop": [ 752121, 752307 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75169", "query_cov": 1.0, "subject_cov": 0.5, "identity": 1.0, "score": 168.0, "evalue": 6.81e-52, "gene_start": 1, "gene_end": 86, "reference_start": 87, "reference_end": 172, "reference_sequence": "MKLQIFNTKLGLLAAKIYWKSWRHLGLSEDEIVSIALHAEHDSKKRYDPKYGLSFETYLKLNGANFIKSSYRSLMNKVDDWIGFKDMKTLEEQNTVSYTPENYLRSVEFREMVHLAFKKAKNEDERQVFALYVKGYKNLEIAKKLNLTTRRVRYLISIFKDHIKVLTKRYGY" }, "paralog": false, "identity": 0.9883720930232558, "score": 337.0, "evalue": 9.65e-121, "description": "Internal stop codon at 752121, 752307. Nonsense mutation around 752121, 752307.", "psc": { "uniref90_id": "UniRef90_P75169", "product": "Uncharacterized protein MG428 homolog", "uniref50_id": "UniRef50_P47667", "go_ids": [ "GO:0003700", "GO:0006352" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75169", "UniRef:UniRef50_P47667", "GO:0003700", "GO:0006352" ] }, "pscc": { "uniref50_id": "UniRef50_P47667", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47667" ], "product": "Uncharacterized protein MG428" } }, "id": "APKECPOBEN_1328", "locus": "GCA002090215_01328" }, { "type": "cds", "sequence": "contig_1", "start": 752582, "stop": 752812, "strand": "-", "frame": 3, "gene": null, "product": "PEP-utilising enzyme C-terminal domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0K9GSR8" ], "nt": "ATGTGTGGTGAGATGGCCGGTGAACCGTTAGCGATTCCCCTACTTTTAGGTTTGGGTCTCAAAGAGTTTTCCATGAGTGCTAGTTCCATGTTTAAGGCGCGGATGATTATCGCTAAGCTCAATTACACAGAGTGTCAGACATTAGCACAAAAAGCATTAACCTTAGCGAACGCCAAGGAGGTGGAAAAGCTAGTGGAAAAATTCTTCAAGAAGAAGGATATCTTCATCTAG", "aa": "MCGEMAGEPLAIPLLLGLGLKEFSMSASSMFKARMIIAKLNYTECQTLAQKALTLANAKEVEKLVEKFFKKKDIFI", "aa_hexdigest": "34a06d14b9bb79439c625a730ce9b8d0", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A6L3V2M9", "query_cov": 0.8552631578947368, "subject_cov": 0.9027777777777778, "identity": 0.569, "score": 69.3, "evalue": 4.51e-14, "valid": false, "product": "PEP-utilising enzyme C-terminal domain-containing protein", "uniref50_id": "UniRef50_A0A0K9GSR8" }, "pscc": { "uniref50_id": "UniRef50_A0A0K9GSR8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0K9GSR8" ], "product": "PEP-utilising enzyme C-terminal domain-containing protein" }, "genes": [], "id": "APKECPOBEN_1329", "locus": "GCA002090215_01329" }, { "type": "cds", "sequence": "contig_1", "start": 752819, "stop": 753340, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02896.24" ], "nt": "GTGTTAGAACAAGCGCACGGTGATTTAGTTATTATCCGCACCCTAGACATTGGTGGGGACAAGAAGCTTAATTACTATGAGTTTCCTCACGAAGATAACCCCTTCTTGGGCTACCGCGCTTTGCGTTTAACCTTAGACAAACAGGACATCTTTAAAACACAATTGCGGGCTTTACTACGTGCTGCTGATCACGGTCAGTTGGGCATTATGTTCCCGATGGTCGCTACCTTGGATGAACTGTTGCAAGCCAAGCAACTGTTAAACCAGGTGCACCAGGAGTTGGGTGGCAATAAACAGTTTAAGCTCGGGATTATGATCGAAATCCCCGCTGCTGTTTTAGCGGCTAACACCTTAAGTCACCACGTGGATTTCTTCTCCATTGGTACCAATGACCTGATCCAGTACAGCTTTGCGGCTGACCGGATGAATAAGAATGTTAGTTATCTCTACCAACCTTTAAACCCAGCTTTATTAAAACTCATTTACCTCACCATTGAAGGGGGTAAAGTGAATGATATTTGA", "aa": "MLEQAHGDLVIIRTLDIGGDKKLNYYEFPHEDNPFLGYRALRLTLDKQDIFKTQLRALLRAADHGQLGIMFPMVATLDELLQAKQLLNQVHQELGGNKQFKLGIMIEIPAAVLAANTLSHHVDFFSIGTNDLIQYSFAADRMNKNVSYLYQPLNPALLKLIYLTIEGGKVNDI", "aa_hexdigest": "0c7ee1613f3aa3f4bc83d4d45e76f441", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02896.24", "name": "PEP-utilizers_C", "length": 174, "aa_cov": 0.976878612716763, "hmm_cov": 1.0, "evalue": 2.6771770016110947e-71, "score": 235.5618133544922, "start": 1, "stop": 169 } ], "seq_stats": { "molecular_weight": 19522.377600000003, "isoelectric_point": 6.038313102722167 }, "id": "APKECPOBEN_1330", "locus": "GCA002090215_01330" }, { "type": "cds", "sequence": "contig_1", "start": 753377, "stop": 754300, "strand": "-", "frame": 3, "gene": "ptsI", "product": "Phosphoenolpyruvate-protein phosphotransferase", "db_xrefs": [ "COG:COG1080", "COG:G", "EC:2.7.3.9", "GO:0005737", "GO:0008965", "GO:0009401", "GO:0016301", "GO:0046872", "SO:0001217", "UniRef:UniRef50_P47668", "UniRef:UniRef90_P75168" ], "nt": "ATGAAAAAACTATCTGGTATCGGTGTATCTGATGGCATGGCCCTAGCCAAAGCCTTCCTTGTTAAAACACCGGAATTCGCTGTTAACAAATACCTCAAGCACCAATTAACGAAGGCGCAGGCCAAAAGACTTTTGGACTCAGCTTTCAAAAAAGCCGTTAAGGACTTAGAGGAAATTAAGGAAATTACCGTTAACAACATTAACACTGAAGCGGGCATGATCTTTGATGCGCACATTCAAATGCTCAACGATCCCACGATCACCGAGCAACTAGAACAACAGTTAGCGCAAAATGTTCATCCTGTTATAGCCGTGGACACTGTCTTTAGCCAAACGGCTACCATGTTTAGCCAAATGCAGGACAAGTATTTCCAGGAACGTGCTGCAGACATTTTGGACTTGCGCCAACGCTTACTAGCTTATTTAACCGGACAAAAACCACATGACCTGGTCAAAATTAAGAGTGATGTCATTATTGTGGCCCATGACTTAACGCCGAGTCAAACTGCTACCCTTAACAAAAAATACGTCAAGGGTTTTTTAACCGAAATAGGTGGTCGTACCAGTCACGCTGCCATTATGGCGCGTTCACTGGAAATTCCCGCAGTGGTGGGCATTAAGGGCATTACTACTAAGGTCAAAGACGGTCAAATAGTTGGGGTTGATGGGCGCAAAGGAATAGCTGGCTTAGACCTAAACTCCAAGGACACTACCGAGTGGAAGAAACAAAAAGCGTTGGAAGAAAAATACCAACAAGAACTCAAGCAGTACACCAACAAGGAAACCGTGACTTTAGATGGTCATGCGGTTGTGGTGGCTGCTAACATTGGTAATGTGAAAGACATGGAATTGGCATGTCAGTACAATACCAACGGGGTTGGTTTATTCCGCACTGAGTTCCTCTACATGAACAGTCAAGAATGA", "aa": "MKKLSGIGVSDGMALAKAFLVKTPEFAVNKYLKHQLTKAQAKRLLDSAFKKAVKDLEEIKEITVNNINTEAGMIFDAHIQMLNDPTITEQLEQQLAQNVHPVIAVDTVFSQTATMFSQMQDKYFQERAADILDLRQRLLAYLTGQKPHDLVKIKSDVIIVAHDLTPSQTATLNKKYVKGFLTEIGGRTSHAAIMARSLEIPAVVGIKGITTKVKDGQIVGVDGRKGIAGLDLNSKDTTEWKKQKALEEKYQQELKQYTNKETVTLDGHAVVVAANIGNVKDMELACQYNTNGVGLFRTEFLYMNSQE", "aa_hexdigest": "91d29755422ad7de7874ab0b52257d8c", "start_type": "ATG", "rbs_motif": "AATAA", "genes": [ "ptsI" ], "truncated": "5-prime", "pseudogene": { "start": 753074, "stop": 754300, "observations": { "mutation": [ 753379 ], "stop": [ 753379 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75168", "query_cov": 1.0, "subject_cov": 0.5367132867132867, "identity": 1.0, "score": 588.0, "evalue": 1.06e-207, "gene_start": 1, "gene_end": 307, "reference_start": 1, "reference_end": 307, "reference_sequence": "MKKLSGIGVSDGMALAKAFLVKTPEFAVNKYLKHQLTKAQAKRLLDSAFKKAVKDLEEIKEITVNNINTEAGMIFDAHIQMLNDPTITEQLEQQLAQNVHPVIAVDTVFSQTATMFSQMQDKYFQERAADILDLRQRLLAYLTGQKPHDLVKIKSDVIIVAHDLTPSQTATLNKKYVKGFLTEIGGRTSHAAIMARSLEIPAVVGIKGITTKVKDGQIVGVDGRKGIAGLDLNSKDTTEWKKQKALEEKYQQELKQYTNKETVTLDGHAVVVAANIGNVKDMELACQYNTNGVGLFRTEFLYMNSQEWPDEETQYQAYKAVLEQAHGDLVIIRTLDIGGDKKLNYYEFPHEDNPFLGYRALRLTLDKQDIFKTQLRALLRAADHGQLGIMFPMVATLDELLQAKQLLNQVHQELGGNKQFKLGIMIEIPAAVLAANTLSHHVDFFSIGTNDLIQYSFAADRMNKNVSYLYQPLNPALLKLIYLTIEGGKVNDIWTGMCGEMAGEPLAIPLLLGLGLKEFSMSASSMFKARMIIAKLNYTECQTLAQKALTLANAKEVEKLVEKFFKKKDIFI" }, "paralog": false, "identity": 0.9975490196078431, "score": 795.0, "evalue": 8.41e-292, "description": "Internal stop codon at 753379. Nonsense mutation around 753379.", "psc": { "uniref90_id": "UniRef90_P75168", "gene": "ptsI", "product": "Phosphoenolpyruvate-protein phosphotransferase", "ec_ids": [ "2.7.3.9" ], "uniref50_id": "UniRef50_P47668", "cog_id": "COG1080", "cog_category": "G", "go_ids": [ "GO:0005737", "GO:0016301", "GO:0046872", "GO:0008965", "GO:0009401" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75168", "EC:2.7.3.9", "UniRef:UniRef50_P47668", "COG:COG1080", "COG:G", "GO:0005737", "GO:0016301", "GO:0046872", "GO:0008965", "GO:0009401" ] }, "pscc": { "uniref50_id": "UniRef50_P47668", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47668" ], "product": "Phosphoenolpyruvate-protein phosphotransferase" } }, "id": "APKECPOBEN_1331", "locus": "GCA002090215_01331" }, { "type": "cds", "sequence": "contig_1", "start": 754305, "stop": 755831, "strand": "-", "frame": 2, "gene": "gpmI", "product": "2,3-bisphosphoglycerate-independent phosphoglycerate mutase", "db_xrefs": [ "COG:COG0696", "COG:G", "EC:5.4.2.12", "GO:0005829", "GO:0006007", "GO:0006096", "GO:0016020", "GO:0030145", "GO:0046537", "GO:0051919", "KEGG:K15633", "RefSeq:WP_010874985.1", "SO:0001217", "UniParc:UPI000012BA19", "UniRef:UniRef100_P75167", "UniRef:UniRef50_P75167", "UniRef:UniRef90_P75167" ], "nt": "ATGCATAAGAAGGTCTTACTAGCCATTTTGGATGGTTATGGTATTAGTAATAAGCAACACGGTAATGCGGTGTACCATGCTAAAACTCCAGCTTTAGACAGTTTGATTAAGGATTATCCCTGTGTCATGTTAGAAGCTTCGGGGGAAGCCGTTGGTCTACCACAAGGACAAATCGGTAACTCGGAAGTGGGTCACCTCAATATTGGGGCTGGTCGAATTGTTTACACTGGTTTATCGTTAATTAACCAAAACATTAAAACCGGGGCCTTTCACCACAACCAGGTGTTACTAGAGGCGATTGCTAGGGCCAAGGCTAACAATGCTAAGTTGCACTTAATTGGTCTGTTTTCCCATGGCGGGGTACACAGTCACATGGACCATTTGTATGCCTTGATTAAACTAGCAGCACCGCAGGTCAAAATGGTGCTCCACCTCTTTGGTGATGGGCGGGATGTAGCACCTTGCACGATGAAGAGTGACCTTGAAGCCTTCATGGTGTTCTTAAAGGATTACCACAATGTCATCATTGGTACCTTAGGTGGGCGTTACTATGGTATGGATCGTGACCAGCGCTGGGACAGAGAAGAGATTGCTTATAATGCCATTTTGGGTAACAGTAAGGCTAGCTTTACTGATCCGGTAGCTTATGTTCAAAGTGCCTATGACCAAAAGGTAACCGATGAGTTTTTGTACCCGGCTGTCAATGGTAATGTAGATAAAGAGCAGTTTGCGCTCAAAGATCATGACAGTGTGATCTTCTTTAACTTCCGTCCTGATCGTGCGCGGCAAATGTCACACATGCTCTTTCAAACAGATTACTATGACTACACCCCTAAAGCAGGACGAAAGTACAACCTCTTCTTTGTCACGATGATGAACTATGAGGGGATTAAACCAAGTGCCGTGGTCTTTCCGCCCGAAACTATTCCCAATACTTTTGGGGAAGTCATTGCGCACAATAAGTTAAAGCAACTGCGAATTGCAGAAACGGAAAAGTACGCCCATGTAACCTTCTTCTTTGATGGTGGAGTGGAAGTGGATCTACCCAATGAAACTAAGTGCATGGTACCTTCGTTAAAGGTGGCTACGTATGACTTAGCACCGGAAATGGCATGTAAGGGGATAACTGATCAATTACTAAATCAAATTAACCAGTTCGATTTGACGGTGTTGAACTTTGCCAACCCTGATATGGTAGGTCATACTGGTAACTATGCCGCTTGTGTGCAGGGCTTAGAGGCCTTGGATGTGCAAATACAGCGCATTATTGATTTCTGCAAGGCTAACCACATTACCCTGTTCTTAACTGCTGACCATGGTAACGCGGAAGAGATGATTGACAGCAACAATAACCCAGTTACCAAACACACTGTCAACAAGGTGCCGTTTGTATGTACTGATACAAACATTGACTTACAACAAGACAGTGCTAGTCTAGCCAATATTGCCCCAACCATTTTGGCTTACTTAGGGCTCAAACAGCCCGCAGAAATGACCGCAAACTCCCTTTTAATAAGTAAAAAGTAA", "aa": "MHKKVLLAILDGYGISNKQHGNAVYHAKTPALDSLIKDYPCVMLEASGEAVGLPQGQIGNSEVGHLNIGAGRIVYTGLSLINQNIKTGAFHHNQVLLEAIARAKANNAKLHLIGLFSHGGVHSHMDHLYALIKLAAPQVKMVLHLFGDGRDVAPCTMKSDLEAFMVFLKDYHNVIIGTLGGRYYGMDRDQRWDREEIAYNAILGNSKASFTDPVAYVQSAYDQKVTDEFLYPAVNGNVDKEQFALKDHDSVIFFNFRPDRARQMSHMLFQTDYYDYTPKAGRKYNLFFVTMMNYEGIKPSAVVFPPETIPNTFGEVIAHNKLKQLRIAETEKYAHVTFFFDGGVEVDLPNETKCMVPSLKVATYDLAPEMACKGITDQLLNQINQFDLTVLNFANPDMVGHTGNYAACVQGLEALDVQIQRIIDFCKANHITLFLTADHGNAEEMIDSNNNPVTKHTVNKVPFVCTDTNIDLQQDSASLANIAPTILAYLGLKQPAEMTANSLLISKK", "aa_hexdigest": "2134cfd9ff5202aa978d0e0c1680bb52", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000012BA19", "ncbi_nrp_id": "WP_010874985.1", "uniref100_id": "UniRef100_P75167", "db_xrefs": [ "SO:0001217", "UniParc:UPI000012BA19", "RefSeq:WP_010874985.1", "UniRef:UniRef100_P75167" ] }, "ips": { "uniref100_id": "UniRef100_P75167", "uniref90_id": "UniRef90_P75167" }, "psc": { "uniref90_id": "UniRef90_P75167", "gene": "gpmI", "product": "2,3-bisphosphoglycerate-independent phosphoglycerate mutase", "ec_ids": [ "5.4.2.12" ], "uniref50_id": "UniRef50_P75167", "cog_id": "COG0696", "cog_category": "G", "kegg_orthology_id": "K15633", "go_ids": [ "GO:0005829", "GO:0006007", "GO:0006096", "GO:0016020", "GO:0030145", "GO:0046537", "GO:0051919" ] }, "pscc": { "uniref50_id": "UniRef50_P75167", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75167" ], "product": "2,3-bisphosphoglycerate-independent phosphoglycerate mutase" }, "genes": [ "gpmI" ], "id": "APKECPOBEN_1332", "locus": "GCA002090215_01332" }, { "type": "cds", "sequence": "contig_1", "start": 755824, "stop": 756006, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00121.24" ], "nt": "GTGTACCTTAGTGAACTGTACAACAAGGAAATTGCCCAACAAACCTCGATTCTCTATGGTGGTTCGGTTGCTAAGGACAACATTAAGGAGTTAGCACAAACCGAGCAAATTGATGGCTTTTTAGTGGGCAAGGCATCTTTGGATGTCAACGACTTTTTAGTAATGGCACAAGTATATGCATAA", "aa": "MYLSELYNKEIAQQTSILYGGSVAKDNIKELAQTEQIDGFLVGKASLDVNDFLVMAQVYA", "aa_hexdigest": "d51d19e6c8eaf0e8e0101c3f8fe5ec8f", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00121.24", "name": "TIM", "length": 55, "aa_cov": 0.9166666666666666, "hmm_cov": 0.9818181818181818, "evalue": 6.895313254826843e-18, "score": 60.47081756591797, "start": 3, "stop": 57 } ], "seq_stats": { "molecular_weight": 6645.474500000002, "isoelectric_point": 4.285114479064942 }, "id": "APKECPOBEN_1333", "locus": "GCA002090215_01333" }, { "type": "cds", "sequence": "contig_1", "start": 756070, "stop": 756492, "strand": "-", "frame": 1, "gene": "tpiA", "product": "triose-phosphate isomerase", "db_xrefs": [ "COG:COG0149", "COG:G", "EC:5.3.1.1", "GO:0004807", "GO:0005829", "GO:0006094", "GO:0006096", "GO:0019563", "GO:0046166", "KEGG:K01803", "SO:0001217", "UniRef:UniRef50_P78010", "UniRef:UniRef90_P78010" ], "nt": "GTGGAGCAATTTAAACAGCACCCTGCTAAAACTAAGGCGGTTTTGGGTATTGCCCCAGTCCACGTCCACTTAACCGAGGTAAACAAAGTCTTGCCTAATAACCTCTTACTGTTAGCCCAAGACGCTAACTTTATTGCTAGTGGTTCTTACACTGGTACGGTGAGCTATACCCAACTGCAAGACATTAAGGTCAACAGTGTCATTATTGGTCACTCGGAACGGCGAAAGTACTTTAACGAAACAGCGCAAGTTATTAATCAAAAACTAAAAGCCTGTTTACAAGCTGGAATGTTAGTCGTGCTCTGTATTGGTGAAACGGAAGGTCAACCAATTAGTTTTCTCAAAGAAGACTTAACCCAAGTCTTGCAAGGCATTGACCTAAGCTTACTCAAACAGTTAGTGATTGCTTACGAACCAATTTGA", "aa": "MEQFKQHPAKTKAVLGIAPVHVHLTEVNKVLPNNLLLLAQDANFIASGSYTGTVSYTQLQDIKVNSVIIGHSERRKYFNETAQVINQKLKACLQAGMLVVLCIGETEGQPISFLKEDLTQVLQGIDLSLLKQLVIAYEPI", "aa_hexdigest": "9c5023fcb743242a885b5d55a7d0cbe1", "start_type": "GTG", "rbs_motif": null, "genes": [ "tpiA" ], "truncated": "both", "pseudogene": { "start": 755824, "stop": 756558, "observations": { "mutation": [ 756072, 756531 ], "stop": [ 756072, 756531 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P78010", "query_cov": 1.0, "subject_cov": 0.5737704918032787, "identity": 0.993, "score": 271.0, "evalue": 1.55e-90, "gene_start": 1, "gene_end": 140, "reference_start": 23, "reference_end": 162, "reference_sequence": "MRTKYLIGNWKTNKDLHQALAFVEQFKQHPAKTKAVLGIAPVHVHLTEVNKVLPNNLLLLAQDANFIASGSYTGTVSYTQLQDIKVNSVIIGHSERRKYFNETAQVINQKLKACLQAGMLVVLCIGETEGQPISFLKEDLTQVLQGIDLSLLKQLVIAYEPIWAIGTGKTATPEIANNTIAQIRVYLSELYNKEIAQQTSILYGGSVAKDNIKELAQTEQIDGFLVGKASLDVNDFLVMAQVYA" }, "paralog": false, "identity": 0.9918032786885246, "score": 466.0, "evalue": 1.43e-169, "description": "Internal stop codon at 756072, 756531. Nonsense mutation around 756072, 756531.", "psc": { "uniref90_id": "UniRef90_P78010", "gene": "tpiA", "product": "triose-phosphate isomerase", "ec_ids": [ "5.3.1.1" ], "uniref50_id": "UniRef50_P78010", "cog_id": "COG0149", "cog_category": "G", "kegg_orthology_id": "K01803", "go_ids": [ "GO:0004807", "GO:0005829", "GO:0006094", "GO:0006096", "GO:0019563", "GO:0046166" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P78010", "EC:5.3.1.1", "UniRef:UniRef50_P78010", "COG:COG0149", "COG:G", "KEGG:K01803", "GO:0004807", "GO:0005829", "GO:0006094", "GO:0006096", "GO:0019563", "GO:0046166" ] }, "pscc": { "uniref50_id": "UniRef50_P78010", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P78010" ], "product": "Triosephosphate isomerase" } }, "id": "APKECPOBEN_1334", "locus": "GCA002090215_01334" }, { "type": "cds", "sequence": "contig_1", "start": 756611, "stop": 757825, "strand": "-", "frame": 3, "gene": "yitT", "product": "putative MG432-like protein", "db_xrefs": [ "COG:COG1284", "COG:S", "GO:0005886", "RefSeq:WP_010874987.1", "SO:0001217", "UniParc:UPI00001397D0", "UniRef:UniRef100_P75166", "UniRef:UniRef50_Q49432", "UniRef:UniRef90_P75166" ], "nt": "ATGCAAGATAAAAACGTCAAAATTCAGGGCAATCTGGTACGGGTACACCTTTCGGGATCGTTTCTGAAGTTCCAGGCAATTTACAAGGTGAAAAAGCTGTACTTACAGCTGTTAATTCTCTCCGTGATTGCCTTCTTTTGGGGCTTGTTAGGAGTTGTGTTTGTCCAGTTTTCTGGATTATATGACATTGGCATTGCTTCCATTAGTCAGGGCTTAGCACGGTTAGCGGATTATTTAATTAGGTCGAACAAGGTCAGTGTGGATGCTGACACCATTTACAACGTCATCTTCTGGTTGAGTCAAATTCTGATTAACATTCCCTTATTTGTTTTGGGTTGGTACAAGATTTCCAAAAAGTTTACCTTGTTAACCCTTTACTTTGTGGTAGTCTCCAACGTTTTTGGGTTTGCCTTCTCTTACATTCCGGGCGTGGAAAACTTCTTCTTGTTTGCTAATTTAACTGAACTTACTAAGGCCAACGGTGGCTTAGAACAAGCGATTAACAACCAAGGGGTGCAACTGATCTTTTGGGAACAAACCGCTGAAAAGCAAATTTCGTTAATGTTCTATGCGCTGATCTGGGGTTTTCTTCAAGCTGTGTTTTACTCAGTTATCCTAATTATTGATGCATCGAGTGGTGGGTTGGACTTTTTGGCCTTCTGGTATTCGGAAAAGAAACACAAGGACATTGGTGGTATTTTGTTTATTGTTAACACCCTTAGTTTCTTGATCGGTTACACCATTGGCACTTACCTTACCGGTAGCTTACTAGCACAAGGCTTTCAAGAAGATAGACAAAAACCGTTTGGAGTGGCTTTTTTCTTGTCCCCTAACTTAGTGTTTACGATTTTCATGAACATTATCTTAGGGATCTTTACCTCCTACTTCTTTCCTAAATACCAGTTTGTCAAAGTGGAAGTGTATGGTAAACACATGGAACAAATGCGCAACTACTTGTTGAGCAGTAACCAGTCCTTTGCGGTCACTATGTTCGAAGTGGAAGGGGGGTACTCGCGCCAAAAGAACCAGGTGTTAGTTACAAACTGTTTGTTTACGAAAACGGCCGAACTTTTAGAAGCTGTTAGACGAGTCGATCCGGATGCTCTGTTCTCAATTACCTTCATTAAAAAGTTGGATGGTTATATCTATGAAAGAAAAGCACCTGATAAAGTAGTCCCACCAGTAAAAGACCCAGTTAAAGCCCAGGAAAATTAA", "aa": "MQDKNVKIQGNLVRVHLSGSFLKFQAIYKVKKLYLQLLILSVIAFFWGLLGVVFVQFSGLYDIGIASISQGLARLADYLIRSNKVSVDADTIYNVIFWLSQILINIPLFVLGWYKISKKFTLLTLYFVVVSNVFGFAFSYIPGVENFFLFANLTELTKANGGLEQAINNQGVQLIFWEQTAEKQISLMFYALIWGFLQAVFYSVILIIDASSGGLDFLAFWYSEKKHKDIGGILFIVNTLSFLIGYTIGTYLTGSLLAQGFQEDRQKPFGVAFFLSPNLVFTIFMNIILGIFTSYFFPKYQFVKVEVYGKHMEQMRNYLLSSNQSFAVTMFEVEGGYSRQKNQVLVTNCLFTKTAELLEAVRRVDPDALFSITFIKKLDGYIYERKAPDKVVPPVKDPVKAQEN", "aa_hexdigest": "6c1b6d1da7ce150ae378c0010c848b87", "start_type": "ATG", "rbs_motif": "AAAA", "ups": { "uniparc_id": "UPI00001397D0", "ncbi_nrp_id": "WP_010874987.1", "uniref100_id": "UniRef100_P75166", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001397D0", "RefSeq:WP_010874987.1", "UniRef:UniRef100_P75166" ] }, "ips": { "uniref100_id": "UniRef100_P75166", "uniref90_id": "UniRef90_P75166" }, "psc": { "uniref90_id": "UniRef90_P75166", "gene": "YitT", "product": "Uncharacterized protein MG432 homolog", "uniref50_id": "UniRef50_Q49432", "cog_id": "COG1284", "cog_category": "S", "go_ids": [ "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_Q49432", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49432" ], "product": "Uncharacterized protein MG432" }, "genes": [ "yitT" ], "id": "APKECPOBEN_1335", "locus": "GCA002090215_01335" }, { "type": "cds", "sequence": "contig_1", "start": 757912, "stop": 758028, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAACAAAAATAGAGCTCATAAAAGAACTACGTAAAACAACACAAGCGAGCATGATGGACTGTAAAAAGGCTTTGGAACAAAATAACGATGATCTCGAAAAGGCCATTAAGTGA", "aa": "MATKIELIKELRKTTQASMMDCKKALEQNNDDLEKAIK", "aa_hexdigest": "f0cb3a4a31ad7258ac8d1163fc305f1b", "start_type": "ATG", "rbs_motif": "AAAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4381.1236, "isoelectric_point": 7.870574378967285 }, "id": "APKECPOBEN_1336", "locus": "GCA002090215_01336" }, { "type": "cds", "sequence": "contig_1", "start": 758029, "stop": 758808, "strand": "+", "frame": 1, "gene": "tsf", "product": "translation elongation factor Ts", "db_xrefs": [ "COG:COG0264", "COG:J", "GO:0003746", "GO:0005737", "GO:0006414", "SO:0001217", "UniRef:UniRef50_P47246", "UniRef:UniRef90_P78009" ], "nt": "TTGAGAGAAAACGGAATTGTTAAATCTGCTAAGAAATTAGGCAAAGTAGCCGCTGATGGTTGCATTGTGTTACACAGCGATCACCACAAGGCAGTGATGGTGGAAATTAATTCTCAAACGGACTTTGTAGCGCGTAGTCAAGAGTTAACGGACTTTGCGCAATTAATGATTAGTGAAGTCTTTAAAAAGGCTACCCCTACCACTACTATTGAAGAAGTAACCCAGTACGAACTCCAAGGCAAAGAAAAGGTAGCAGAACGTTTGGCTTTAGTCGCTTCCAAAACTGATGAAAAGATTGTGTTGCGCCGCTTAATGGTGTTTGAAAGCAAAACTAACCACATCTTTAGCTACCTCCACGCTAACAAACGGATTGGCGTAATCTTGGAAGTGGAAGGCAAGTTTGATGAACAAGATGGTAAACACTTAGCAATGCACATTGCCGCTAACTCACCGCAGTTCATTGATCAGGACAATGTGGATCAAACATGGTTAGCTAACGAAACCAGCATCATTAAGTCCCAAGCCAAACTAGAAGTCCAAGACAATCCGAAGAAAGCAGCGTTCTTAGACAAAACTATTGCTGGACGCGTGAACAAACTCTTAATTGACATTTGTCTGGTAAACCAAAAGTACTTGGTCGATGAATCCAAAACAGTTGGTCAATTCTTAAAGGAAAAGAACAGTAAAGTAATCCATTTTGTGCGCTTTGAAGTTGGTGAAGGCATTGTCAAAGAAGCAGTTGACTTCGCTAGTGAAGTAAGTGCGCAAATGAAAAAATAA", "aa": "MRENGIVKSAKKLGKVAADGCIVLHSDHHKAVMVEINSQTDFVARSQELTDFAQLMISEVFKKATPTTTIEEVTQYELQGKEKVAERLALVASKTDEKIVLRRLMVFESKTNHIFSYLHANKRIGVILEVEGKFDEQDGKHLAMHIAANSPQFIDQDNVDQTWLANETSIIKSQAKLEVQDNPKKAAFLDKTIAGRVNKLLIDICLVNQKYLVDESKTVGQFLKEKNSKVIHFVRFEVGEGIVKEAVDFASEVSAQMKK", "aa_hexdigest": "bdf2184fb757c8bf8769a32d2c5e8e8d", "start_type": "TTG", "rbs_motif": "AAAA", "psc": { "uniref90_id": "UniRef90_P78009", "query_cov": 1.0, "subject_cov": 0.8691275167785235, "identity": 0.996, "score": 496.0, "evalue": 2.1e-176, "valid": true, "gene": "tsf", "product": "translation elongation factor Ts", "uniref50_id": "UniRef50_P47246", "cog_id": "COG0264", "cog_category": "J", "go_ids": [ "GO:0003746", "GO:0005737", "GO:0006414" ] }, "pscc": { "uniref50_id": "UniRef50_P47246", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47246" ], "product": "Elongation factor Ts" }, "genes": [ "tsf" ], "id": "APKECPOBEN_1337", "locus": "GCA002090215_01337" }, { "type": "cds", "sequence": "contig_1", "start": 758810, "stop": 759517, "strand": "+", "frame": 2, "gene": "pyrH", "product": "UMP kinase", "db_xrefs": [ "COG:COG0528", "COG:F", "EC:2.7.4.22", "GO:0005524", "GO:0005737", "GO:0006221", "GO:0006225", "GO:0015949", "GO:0033862", "GO:0044210", "KEGG:K09903", "SO:0001217", "UniParc:UPI0001DD6475", "UniRef:UniRef100_A0AAN4XGQ3", "UniRef:UniRef50_P47672", "UniRef:UniRef90_P75165" ], "nt": "ATGCGCCTAAAGATCCTAATCAAACTAAGCGGTGCTGGCATGAGCGCTGATAGTTCCGAACCGTTTTCCAACCAATTTTTAGAAACTGTCATCGCTCAACTAAAACAACTAGTACCCAACTACCAAATAGGCATTGTGATTGGTGGGGGCAACATTATGCGCGGTAAGTCTTGTAGTGACTACAACATTACCGAAATAGCAGGGCACCACTTGGGCATTATGGCCACGGTTATTAACGGCGCTTTTTTAAAGGCTAAGTTTGATAGTCATAAACTTAACAGCACCCTGTTGAGTGCAGTGAGCTGTCCTAGTTTAGCGACCCACATTGTGTCCCAGGCCACGATTGATGATGCCTTTAAAAACCATGACATTGTCATTTTTGCGGGCGGTACTGGTAACCCGTACTTTTCGACTGATACCGCTGTGGCGTTGCGTGCTACCCAAATGCAAGCCGATATTATTCTAATTGGCAAGAATGGTGTTGATGGGGTTTACACTGCGGATCCTAAAAAGGATAAGCATGCTAAGTTTTTAGCTAGCTTAACTTACGCTGAAGCGATTAAGAATGACCTGCAAATCATGGACATTACCGCCTTTACAATGTGTAAGGAAAATAACTTAAAGGTGATCATATTTAACATCAATGCCGAGCATGCCATCATTAAGGCATTGAGCAAACAAGGTAAATACACCTTAATAGAAAAATAA", "aa": "MRLKILIKLSGAGMSADSSEPFSNQFLETVIAQLKQLVPNYQIGIVIGGGNIMRGKSCSDYNITEIAGHHLGIMATVINGAFLKAKFDSHKLNSTLLSAVSCPSLATHIVSQATIDDAFKNHDIVIFAGGTGNPYFSTDTAVALRATQMQADIILIGKNGVDGVYTADPKKDKHAKFLASLTYAEAIKNDLQIMDITAFTMCKENNLKVIIFNINAEHAIIKALSKQGKYTLIEK", "aa_hexdigest": "510b04b8c0c148efd9efe0ac5d887d14", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD6475", "uniref100_id": "UniRef100_A0AAN4XGQ3", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD6475", "UniRef:UniRef100_A0AAN4XGQ3" ] }, "ips": { "uniref100_id": "UniRef100_A0AAN4XGQ3", "uniref90_id": "UniRef90_P75165" }, "psc": { "uniref90_id": "UniRef90_P75165", "gene": "pyrH", "product": "UMP kinase", "ec_ids": [ "2.7.4.22" ], "uniref50_id": "UniRef50_P47672", "cog_id": "COG0528", "cog_category": "F", "kegg_orthology_id": "K09903", "go_ids": [ "GO:0005524", "GO:0005737", "GO:0006221", "GO:0006225", "GO:0015949", "GO:0033862", "GO:0044210" ] }, "pscc": { "uniref50_id": "UniRef50_P47672", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47672" ], "product": "Uridylate kinase" }, "genes": [ "pyrH" ], "id": "APKECPOBEN_1338", "locus": "GCA002090215_01338" }, { "type": "cds", "sequence": "contig_1", "start": 759689, "stop": 760363, "strand": "+", "frame": 2, "gene": null, "product": "ATP-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000A4BAEE8", "UniRef:UniRef90_UPI000A4BAEE8" ], "nt": "TTGATTGCCAATGCGATTGATGAACACCGCTTAAGCAACACCGCTTTCCCCGTAATTGAATTGCAAAAAGGCTTTCTAAACTCTTCTTTAGTGATTAAAGATTATGGTAGGGGTATTAAGTCCAATCACTTTATCCAAAACGAAAGTAGGGAAAAGGTTCAAAGTGAAAAAACTATTGGTAAGTTTGGCATTGGTCTCAAAGATGCCATAGCTGTTCTTTTCCGTCATAATGTCAAAGTTAGCTTTACCAGTAGTGAAGGCACTTTTACCCCTGTTGAGCGAATGAAGGAAGGTATGAAAGACGGCACCAAAACAATTCAAATAACAGTGGATGAAACCAAGAAGATTGACAAGGGCACTGACATCCTCATTAGCAAGATTAGTAGAAGTGATTACGAAAAAGCCATTGCTATTTTTTTGGAATTAAGAACTGGCTATCAAAAACTGGCTTCTTCCAAAAAGGGTGATATTTATCGCAGTGAAAATGGTTCAGAGATCTTTCTTAACGGGATGAAAATCGGAACCGATGAAAACTTCTTGTTTTCTTATGACATTAAAGAACCCAATAAGAAATTGCAAAAATCCTTAAACCGGGAGCGTAAAACACTTTCCCGTGATAGCTACCGTGACAATATCATTTCCATTTTGAAGTCCAGCATTAATAAAAATACCTAA", "aa": "MIANAIDEHRLSNTAFPVIELQKGFLNSSLVIKDYGRGIKSNHFIQNESREKVQSEKTIGKFGIGLKDAIAVLFRHNVKVSFTSSEGTFTPVERMKEGMKDGTKTIQITVDETKKIDKGTDILISKISRSDYEKAIAIFLELRTGYQKLASSKKGDIYRSENGSEIFLNGMKIGTDENFLFSYDIKEPNKKLQKSLNRERKTLSRDSYRDNIISILKSSINKNT", "aa_hexdigest": "965c3833b9988e462c88120886eed234", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI000A4BAEE8", "query_cov": 1.0, "subject_cov": 0.8, "identity": 0.991, "score": 421.0, "evalue": 6.79e-148, "valid": true, "product": "ATP-binding protein", "uniref50_id": "UniRef50_UPI000A4BAEE8" }, "pscc": { "uniref50_id": "UniRef50_UPI000A4BAEE8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000A4BAEE8" ], "product": "ATP-binding protein" }, "genes": [], "id": "APKECPOBEN_1339", "locus": "GCA002090215_01339" }, { "type": "cds", "sequence": "contig_1", "start": 760577, "stop": 760741, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAAATGATGCCGAATTTAAAAGCTATACCCTCAACGATTTTGGTGATAGATTTGTCAATGAATTCCAAACGGAAGAGGTGCCATTCCACAAATTAACTGAGATCGAAAAAGACAACTGGAATTGGGTAATGGCTAAGGTCAAAGAACTTACCAGGGTTTGA", "aa": "MENDAEFKSYTLNDFGDRFVNEFQTEEVPFHKLTEIEKDNWNWVMAKVKELTRV", "aa_hexdigest": "65a8f1754f43d7a31f7033ce0dddd929", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6570.2459, "isoelectric_point": 4.637345695495605 }, "id": "APKECPOBEN_1340", "locus": "GCA002090215_01340" }, { "type": "cds", "sequence": "contig_1", "start": 761157, "stop": 761258, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI00035C1894", "UniRef:UniRef100_A0AB38W6X6", "UniRef:UniRef50_A0AB38W6X6", "UniRef:UniRef90_A0AB38W6X6" ], "nt": "ATGTTTTATCTCGATTTAAACATTCAAAATATTCTTACCAATTGGGAGATAGCTGATGCTGTTCGCGAACTGATCGCTAACGCCATTGATGAGCACATTTAA", "aa": "MFYLDLNIQNILTNWEIADAVRELIANAIDEHI", "aa_hexdigest": "be05d12b720a62bb5b8bcb4ad1f3b971", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00035C1894", "uniref100_id": "UniRef100_A0AB38W6X6", "db_xrefs": [ "SO:0001217", "UniParc:UPI00035C1894", "UniRef:UniRef100_A0AB38W6X6" ] }, "ips": { "uniref100_id": "UniRef100_A0AB38W6X6", "uniref90_id": "UniRef90_A0AB38W6X6" }, "psc": { "uniref90_id": "UniRef90_A0AB38W6X6", "uniref50_id": "UniRef50_A0AB38W6X6" }, "pscc": { "uniref50_id": "UniRef50_A0AB38W6X6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W6X6" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3872.3594999999987, "isoelectric_point": 4.0500284194946286 }, "id": "APKECPOBEN_1341", "locus": "GCA002090215_01341" }, { "type": "cds", "sequence": "contig_1", "start": 761462, "stop": 762019, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAX0SSK4", "UniRef:UniRef90_A0AAX0SSK4" ], "nt": "GTGAGTTTTACAAATCAGCAAGGTACTTTTATTCCTGTAGAACGGAGTAAAACTGAACTGGAAGGTAGCATCAAAACAATTCAAATGGAAGTTGATCCAGCTGAACAAATTGAAAAAGCGGGTACCTTAATCACAATTGAAGGATTGAAAAAAGATGACTATGACAAAGCTATTGTTAATTTCTTAGCTCTACGGGAAGATTTGCAATTATTAGCTGCTTCACCCAAAGGCGATGTTTACCGTAATACATCAGGTAACGGCGCAGAAATTTTTCTCAACGGGATGAAAATTGCAACCGATGAAGACTTTCTCTTTTCCTATCACATTAAAGAACCTAACAAAAAGTTACAGCGTTCCCTTAACCGGGAAAATAAAAATTTACCAAGAGATTGTTATCGTGAAAACATCATCACCATTCTTAAGTCCAACATTAACAATAGAACACAAACACTTATTGATGAATTAATAGATTCACGTGACCAATATGACAATGGCGAGTGGAGTTTTATTGATGTCAAAAAACTAATTGGTTTAAACACAAACAGAAATATTTTGTGA", "aa": "MSFTNQQGTFIPVERSKTELEGSIKTIQMEVDPAEQIEKAGTLITIEGLKKDDYDKAIVNFLALREDLQLLAASPKGDVYRNTSGNGAEIFLNGMKIATDEDFLFSYHIKEPNKKLQRSLNRENKNLPRDCYRENIITILKSNINNRTQTLIDELIDSRDQYDNGEWSFIDVKKLIGLNTNRNIL", "aa_hexdigest": "c4432440b4e568a12ed0b40007efae01", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0AAX0SSK4", "query_cov": 1.0, "subject_cov": 0.9296482412060302, "identity": 0.995, "score": 357.0, "evalue": 2.2e-124, "valid": true, "uniref50_id": "UniRef50_A0AAX0SSK4" }, "pscc": { "uniref50_id": "UniRef50_A0AAX0SSK4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAX0SSK4" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 21185.706700000017, "isoelectric_point": 5.207952308654785 }, "id": "APKECPOBEN_1342", "locus": "GCA002090215_01342" }, { "type": "cds", "sequence": "contig_1", "start": 762071, "stop": 762310, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATACCAAAAAATATGAAATTTTGGCGTTAAATTCCTTGCAATACAGAAGCATGGAAAACGATGATCGTTTAAAAAAGCAAACACTGATGCATGTAAGTGAAAAGTTAAAACAACAAAGAATTGAAGAAGAGGCTGAGAAAATCAAAGTTAAAGAACAAAAGCCACGAAAACGATTTGAGGAAGAAGATTTACCAATAGAAGATTTAAATCCAATTGAAAGAGAAGGTTGGGATTGA", "aa": "MDTKKYEILALNSLQYRSMENDDRLKKQTLMHVSEKLKQQRIEEEAEKIKVKEQKPRKRFEEEDLPIEDLNPIEREGWD", "aa_hexdigest": "50ffb4e4359e1d44410c4673845b3261", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9659.859599999996, "isoelectric_point": 5.302191352844238 }, "id": "APKECPOBEN_1343", "locus": "GCA002090215_01343" }, { "type": "cds", "sequence": "contig_1", "start": 762314, "stop": 762604, "strand": "+", "frame": 2, "gene": null, "product": "SprT-like domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAX0S5E5", "UniRef:UniRef90_A0AAX0S5E5" ], "nt": "ATGGAAAAGGCCAGAGAATTATGTGGTTTTATAAGAGGATGGGAAAAACTTTATGAAGAATATCAGTTTGTTCTAATGGAAAAGAATCACAAATATGTCGGGCTTTGTTACACCGATCAAAAAATTATCAAACTCTCAAGAGGAATATTGAAAGATGAATACAGTTTATTGAATACCTTAGTGCATGAAATTTGTCATGCAACAACTAATGGTCGAGACGGAACGAAGAAGTTTGAAAGGGGTCTAACTGATGCTTTTCATCCATTATTTAAACTAGGGCAAAGTAAATAA", "aa": "MEKARELCGFIRGWEKLYEEYQFVLMEKNHKYVGLCYTDQKIIKLSRGILKDEYSLLNTLVHEICHATTNGRDGTKKFERGLTDAFHPLFKLGQSK", "aa_hexdigest": "92c114f8ac44fdc5a111a3d047cd05a7", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 762020, "stop": 762604, "observations": { "mutation": [ 762308 ], "stop": [ 762308 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAX0S5E5", "query_cov": 1.0, "subject_cov": 0.4485981308411215, "identity": 1.0, "score": 206.0, "evalue": 6.84e-66, "gene_start": 1, "gene_end": 96, "reference_start": 119, "reference_end": 214, "reference_sequence": "MEFYDVKKLIGLNTNRNILWADSSSKNIEKLIYSLYGMDTKKYEILALNSLQYRSMENDDRLKKQTLMHVSEKLKQQRIEEEAEKIKVKEQKPRKRFEEEDLPIEDLNPIEREGWDWAMEKARELCGFIRGWEKLYEEYQFVLMEKNHKYVGLCYTDQKIIKLSRGILKDEYSLLNTLVHEICHATTNGRDGTKKFERGLTDAFHPLFKLGQSK" }, "paralog": false, "identity": 0.9948453608247423, "score": 391.0, "evalue": 3.06e-141, "description": "Internal stop codon at 762308. Nonsense mutation around 762308.", "psc": { "uniref90_id": "UniRef90_A0AAX0S5E5", "product": "SprT-like domain-containing protein", "uniref50_id": "UniRef50_A0AAX0S5E5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAX0S5E5", "UniRef:UniRef50_A0AAX0S5E5" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAX0S5E5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAX0S5E5" ], "product": "SprT-like domain-containing protein" } }, "id": "APKECPOBEN_1344", "locus": "GCA002090215_01344" }, { "type": "cds", "sequence": "contig_1", "start": 762753, "stop": 763235, "strand": "+", "frame": 3, "gene": "frr", "product": "ribosome recycling factor", "db_xrefs": [ "COG:COG0233", "COG:J", "GO:0005737", "GO:0006412", "GO:0006415", "GO:0008135", "GO:0043023", "SO:0001217", "UniRef:UniRef50_P47673", "UniRef:UniRef90_P75161" ], "nt": "TTGAGCAAAATCCGTACTGGTAGACCTAATCCAAAACTGTTTGATAATCTCCTTGTGGAAAGCTATGGTGACAGAATGCCAATGGTAGCATTAGCACAAATTGCGGTAAATCCACCGCGGGAAATTGTGATCAAGCCGTTTGACGTTAAAAACAATATTAATGCCATCTACAGCGAAATTCAACGTGCTAACTTAGGTGTACAACCAGTGATTGACGGAGATAAAATTCGCATTAACTTTCCCCCAATGACCCAAGAAAGTCGTTTGGAAAGCATTAAGCAAGCTAAAAAAGTAGTGGAACAAATCCACCAAGAGTTGCGCAGTGTGCGACGTGATACCCTTCAAATGATTAAGAAGGATGATCATAAAGACGAAGACTTTGAAGAATTCTTAAAAGAAGAAGTAGAAAAAGTTAACAAGCAGTATATTGCACAGTTAGAAACTATTCAAAAACAAAAAGAAAAAGAACTATTAGTGGTCTAA", "aa": "MSKIRTGRPNPKLFDNLLVESYGDRMPMVALAQIAVNPPREIVIKPFDVKNNINAIYSEIQRANLGVQPVIDGDKIRINFPPMTQESRLESIKQAKKVVEQIHQELRSVRRDTLQMIKKDDHKDEDFEEFLKEEVEKVNKQYIAQLETIQKQKEKELLVV", "aa_hexdigest": "4337bbbf3b18ffb09f5b7f69afaab4a0", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75161", "query_cov": 1.0, "subject_cov": 0.8648648648648649, "identity": 0.988, "score": 298.0, "evalue": 1.02e-101, "valid": true, "gene": "frr", "product": "ribosome recycling factor", "uniref50_id": "UniRef50_P47673", "cog_id": "COG0233", "cog_category": "J", "go_ids": [ "GO:0005737", "GO:0006412", "GO:0006415", "GO:0008135", "GO:0043023" ] }, "pscc": { "uniref50_id": "UniRef50_P47673", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47673" ], "product": "Ribosome-recycling factor" }, "genes": [ "frr" ], "id": "APKECPOBEN_1345", "locus": "GCA002090215_01345" }, { "type": "cds", "sequence": "contig_1", "start": 763235, "stop": 763351, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATCTGAAAGATAATTCCTTCGCTAAAAAACGATCGACAGTGTTCGTTGTTTTGCTCATTGTCTTTTGTTTTTTTTTAATGTTTTCAGCCTTTGCTGACGGCTTTAACTTTTGA", "aa": "MDLKDNSFAKKRSTVFVVLLIVFCFFLMFSAFADGFNF", "aa_hexdigest": "630807abe7f3f7807ea1454ee89a90fc", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4417.217199999999, "isoelectric_point": 7.933946800231935 }, "id": "APKECPOBEN_1346", "locus": "GCA002090215_01346" }, { "type": "cds", "sequence": "contig_1", "start": 763439, "stop": 763648, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCATCCTGACTTTAAGGCAGTCCGTTTTGCTTTTGGCTTAGTGATAGTTTTATTTATTAGCGTTATTGCCGTTTTAATGAATTGGGAGTTGTCTAACAGTATCTTTAAAAACTATGAAAAGCTCAACTTATCCTTATCACTACTTAGTGGAATTATGGTTTCAGGTGGGATGATTCCAACCTTTTTCGTAATCTATTTCCGTGAATGA", "aa": "MHPDFKAVRFAFGLVIVLFISVIAVLMNWELSNSIFKNYEKLNLSLSLLSGIMVSGGMIPTFFVIYFRE", "aa_hexdigest": "7833b4eeb50329d2239de1359567d57b", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7876.366299999994, "isoelectric_point": 8.16248722076416 }, "id": "APKECPOBEN_1347", "locus": "GCA002090215_01347" }, { "type": "cds", "sequence": "contig_1", "start": 763691, "stop": 764167, "strand": "+", "frame": 2, "gene": "cdsA", "product": "Putative phosphatidate cytidylyltransferase", "db_xrefs": [ "COG:COG0575", "COG:I", "EC:2.7.7.41", "GO:0004605", "GO:0005886", "GO:0016024", "KEGG:K00981", "SO:0001217", "UniRef:UniRef50_Q49433", "UniRef:UniRef90_P75160" ], "nt": "ATGATTGTCTTTTTATGGGCAGTGTACATGATTAGCACTTCCTTCATAAAGATCCGACCATCATTGCAAGTGATTTATAGCCTAGGTGCAGTGATCTGTTTCATCGCTTGCATAGGTACAATTTACTTTTCCGTAATTAGGGGATGGACTACCATTTTTCTCTTAATTGCTATTGGCGTTTGCACTGATACCTTTGCTTATCTGTTTGGTAAACGTTTTGGAAAAAATCCCCTCATTAAAATTTCTCCCTCAAAAACTTGGGAAGGTGCTTTCTTTGGGGTTACTGGTACAGTTTTAACCATTTCTATTATTTGTGTTTTGTACTCGATCCCTAACTATGTAAGACAACCTAGCATAAAGGATGCAAGTAAGACAGCACTACAAACACCACAAAACTATGATGTGCATAACCTTATTACCAATGTCTTTCTCATCTCCTTCATTTCCGGTGGTAGTACCTTTTACATCTACTGGTGA", "aa": "MIVFLWAVYMISTSFIKIRPSLQVIYSLGAVICFIACIGTIYFSVIRGWTTIFLLIAIGVCTDTFAYLFGKRFGKNPLIKISPSKTWEGAFFGVTGTVLTISIICVLYSIPNYVRQPSIKDASKTALQTPQNYDVHNLITNVFLISFISGGSTFYIYW", "aa_hexdigest": "c40ab368dd201c84e57d8d7d9d61fa57", "start_type": "ATG", "rbs_motif": null, "genes": [ "cdsA" ], "truncated": "both", "pseudogene": { "start": 763391, "stop": 764422, "observations": { "mutation": [ 763646, 763664, 763670, 764165 ], "stop": [ 763646, 763664, 763670, 764165 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75160", "query_cov": 1.0, "subject_cov": 0.4, "identity": 1.0, "score": 312.0, "evalue": 2.44e-104, "gene_start": 1, "gene_end": 158, "reference_start": 153, "reference_end": 310, "reference_sequence": "MDLKDNSFAKKRSTVFVVLLIVFCFFLMFSAFADGFNFWSPWSTDFNSRVIHIKSDGNIETTSIVSHELHPDFKAVRFAFGLVIVLFISVIAVLMNWELSNSIFKNYEKLNLSLSLLSGIMVSGGMIPTFFVIYFREWNATVNWIWTASFAGMIVFLWAVYMISTSFIKIRPSLQVIYSLGAVICFIACIGTIYFSVIRGWTTIFLLIAIGVCTDTFAYLFGKRFGKNPLIKISPSKTWEGAFFGVTGTVLTISIICVLYSIPNYVRQPSIKDASKTALQTPQNYDVHNLITNVFLISFISGGSTFYIYWWVSTLALIFTASIFAIGGDLFFSYIKRLTKIKDFSKLLGKHGGILDRFDSSSFLISFFFIYHVIAGISSNQRLLMEPNTYFSAVS" }, "paralog": false, "identity": 0.9883381924198251, "score": 662.0, "evalue": 1.57e-244, "description": "Internal stop codon at 763646, 763664, 763670, 764165. Nonsense mutation around 763646, 763664, 763670, 764165.", "psc": { "uniref90_id": "UniRef90_P75160", "gene": "cdsA", "product": "Putative phosphatidate cytidylyltransferase", "ec_ids": [ "2.7.7.41" ], "uniref50_id": "UniRef50_Q49433", "cog_id": "COG0575", "cog_category": "I", "kegg_orthology_id": "K00981", "go_ids": [ "GO:0005886", "GO:0004605", "GO:0016024" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75160", "EC:2.7.7.41", "UniRef:UniRef50_Q49433", "COG:COG0575", "COG:I", "KEGG:K00981", "GO:0005886", "GO:0004605", "GO:0016024" ] }, "pscc": { "uniref50_id": "UniRef50_Q49433", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49433" ], "product": "Putative phosphatidate cytidylyltransferase" } }, "id": "APKECPOBEN_1348", "locus": "GCA002090215_01348" }, { "type": "cds", "sequence": "contig_1", "start": 764526, "stop": 765017, "strand": "+", "frame": 3, "gene": "hsdS", "product": "Putative type I specificity subunit S-MpnORF638P", "db_xrefs": [ "COG:COG0732", "COG:V", "EC:3.1.21.3", "GO:0003677", "GO:0009307", "KEGG:K01154", "SO:0001217", "UniRef:UniRef50_Q49434", "UniRef:UniRef90_P75159" ], "nt": "ATGCTCGAAAAAGAGCTATTGGCGCCCGATGGTAAATACGAATACTTTAACGGTGGTATTAAAGCCTCTGGACGCACCAATGAGTTCAATACCTTTAAGAACACCATTAGCATTATCATAGGCGGTTCTTGTGGTTATGTCAGATTAGCCGATAAGGATTACTTCTGTGGCCAGAGTAGCTGTACCCTAACTGTTTTAGATCCCCTGGAAATTGACCTCAAGTTTGCTTATTATGCACTTAAATCACAAGAGGAAAAAATTACCAGTTTAGCTAGCGGAACTACCATTAAAAACATTCGCCTAAGTGATTTAAAGGATTTACCTATTCCGCTTGTTAAAAGCATTCAGGATCAACGAACTATTGCACATGCTTTAAGTGTGTTTGACCTTCGCATCGAACACCTCAATGAATTAATTGAAGTAAACCGTAAGCTCAGAGACGAGTATGCCCACAAATTATTTACCTTAGATCCAGACTTCTTAACACACTGA", "aa": "MLEKELLAPDGKYEYFNGGIKASGRTNEFNTFKNTISIIIGGSCGYVRLADKDYFCGQSSCTLTVLDPLEIDLKFAYYALKSQEEKITSLASGTTIKNIRLSDLKDLPIPLVKSIQDQRTIAHALSVFDLRIEHLNELIEVNRKLRDEYAHKLFTLDPDFLTH", "aa_hexdigest": "a1858b4425475498eaa5bee35fab524d", "start_type": "ATG", "rbs_motif": null, "genes": [ "hsdS" ], "truncated": "both", "pseudogene": { "start": 764442, "stop": 765320, "observations": { "mutation": [ 764478, 765015 ], "stop": [ 764478, 765015 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75159", "query_cov": 1.0, "subject_cov": 0.43466666666666665, "identity": 1.0, "score": 328.0, "evalue": 6.72e-111, "gene_start": 1, "gene_end": 163, "reference_start": 29, "reference_end": 191, "reference_sequence": "MTPKLKLNTNSNWTKKTLGSLFELKKGEMLEKELLAPDGKYEYFNGGIKASGRTNEFNTFKNTISIIIGGSCGYVRLADKDYFCGQSSCTLTVLDPLEIDLKFAYYALKSQEEKITSLASGTTIKNIRLSDLKDLPIPLVKSIQDQRTIAHALSVFDLRIEHLNELIEVNRKLRDEYAHKLFTLDPDFLTHWNLHELHEQMGEISLGEVFHLKSGKYLKADERFEDGKFPYYGAGIESTSFVNEPNTKGDTLSMIANGYSIGNIRYHTIPWFNGTGGIAMEALKPNKTYVPFFYCALKYMQKDLKERFKRDESPFISLKLAGEIKVPFVKSFALQRKAGKIIYLLDKTLEECKEEAKSLISIRDNLLGKLFPTLS" }, "paralog": false, "identity": 0.9931506849315068, "score": 584.0, "evalue": 5.34e-214, "description": "Internal stop codon at 764478, 765015. Nonsense mutation around 764478, 765015.", "psc": { "uniref90_id": "UniRef90_P75159", "gene": "HsdS", "product": "Putative type I specificity subunit S.MpnORF638P", "ec_ids": [ "3.1.21.3" ], "uniref50_id": "UniRef50_Q49434", "cog_id": "COG0732", "cog_category": "V", "kegg_orthology_id": "K01154", "go_ids": [ "GO:0003677", "GO:0009307" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75159", "EC:3.1.21.3", "UniRef:UniRef50_Q49434", "COG:COG0732", "COG:V", "KEGG:K01154", "GO:0003677", "GO:0009307" ] }, "pscc": { "uniref50_id": "UniRef50_Q49434", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49434" ], "product": "Putative type I specificity subunit S.MgeORF438P" } }, "id": "APKECPOBEN_1349", "locus": "GCA002090215_01349" }, { "type": "cds", "sequence": "contig_1", "start": 765042, "stop": 765569, "strand": "+", "frame": 3, "gene": "hsdS", "product": "Putative type I specificity subunit S-MpnORF638P", "db_xrefs": [ "COG:COG0732", "COG:V", "EC:3.1.21.3", "GO:0003677", "GO:0009307", "KEGG:K01154", "SO:0001217", "UniRef:UniRef50_Q49434", "UniRef:UniRef90_P75159" ], "nt": "ATGGGTGAAATTAGTTTAGGTGAAGTGTTTCACTTAAAGAGTGGTAAATACTTAAAGGCTGATGAGCGCTTTGAGGATGGCAAATTCCCTTATTATGGTGCTGGTATTGAAAGCACTAGCTTTGTCAATGAACCCAACACCAAAGGTGATACTCTTTCAATGATTGCTAACGGTTACTCCATCGGAAACATTCGCTACCATACTATTCCTTGGTTTAATGGTACTGGTGGTATTGCCATGGAAGCATTAAAACCTAATAAAACCTATGTGCCGTTCTTTTACTGTGCTCTAAAATACATGCAAAAGGATCTAAAGGAACGCTTTAAACGTGATGAATCACCGTTTATTTCCTTAAAACTAGCCGGTGAAATTAAGGTTCCTTTCGTTAAATCGTTTGCACTCCAAAGAAAAGCCGGTAAGATTATTTACTTGTTAGATAAAACTCTTGAAGAATGCAAAGAAGAAGCTAAGTCTTTAATCAGTATTCGCGATAACTTATTGGGCAAATTGTTCCCAACACTATCTTAA", "aa": "MGEISLGEVFHLKSGKYLKADERFEDGKFPYYGAGIESTSFVNEPNTKGDTLSMIANGYSIGNIRYHTIPWFNGTGGIAMEALKPNKTYVPFFYCALKYMQKDLKERFKRDESPFISLKLAGEIKVPFVKSFALQRKAGKIIYLLDKTLEECKEEAKSLISIRDNLLGKLFPTLS", "aa_hexdigest": "510dd43ea5a669ff1edb319c07327d84", "start_type": "ATG", "rbs_motif": null, "genes": [ "hsdS" ], "truncated": "5-prime", "pseudogene": { "start": 764742, "stop": 765569, "observations": { "mutation": [ 765015 ], "stop": [ 765015 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75159", "query_cov": 1.0, "subject_cov": 0.4666666666666667, "identity": 1.0, "score": 356.0, "evalue": 1.28e-121, "gene_start": 1, "gene_end": 175, "reference_start": 201, "reference_end": 375, "reference_sequence": "MTPKLKLNTNSNWTKKTLGSLFELKKGEMLEKELLAPDGKYEYFNGGIKASGRTNEFNTFKNTISIIIGGSCGYVRLADKDYFCGQSSCTLTVLDPLEIDLKFAYYALKSQEEKITSLASGTTIKNIRLSDLKDLPIPLVKSIQDQRTIAHALSVFDLRIEHLNELIEVNRKLRDEYAHKLFTLDPDFLTHWNLHELHEQMGEISLGEVFHLKSGKYLKADERFEDGKFPYYGAGIESTSFVNEPNTKGDTLSMIANGYSIGNIRYHTIPWFNGTGGIAMEALKPNKTYVPFFYCALKYMQKDLKERFKRDESPFISLKLAGEIKVPFVKSFALQRKAGKIIYLLDKTLEECKEEAKSLISIRDNLLGKLFPTLS" }, "paralog": false, "identity": 0.9963636363636363, "score": 547.0, "evalue": 4.97e-199, "description": "Internal stop codon at 765015. Nonsense mutation around 765015.", "psc": { "uniref90_id": "UniRef90_P75159", "gene": "HsdS", "product": "Putative type I specificity subunit S.MpnORF638P", "ec_ids": [ "3.1.21.3" ], "uniref50_id": "UniRef50_Q49434", "cog_id": "COG0732", "cog_category": "V", "kegg_orthology_id": "K01154", "go_ids": [ "GO:0003677", "GO:0009307" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75159", "EC:3.1.21.3", "UniRef:UniRef50_Q49434", "COG:COG0732", "COG:V", "KEGG:K01154", "GO:0003677", "GO:0009307" ] }, "pscc": { "uniref50_id": "UniRef50_Q49434", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49434" ], "product": "Putative type I specificity subunit S.MgeORF438P" } }, "id": "APKECPOBEN_1350", "locus": "GCA002090215_01350" }, { "type": "cds", "sequence": "contig_1", "start": 765566, "stop": 766174, "strand": "-", "frame": 3, "gene": null, "product": "putative protein MPN_639", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75158", "UniRef:UniRef90_P75158" ], "nt": "TTGCTTTCACAGAGAATATTAGAACGTTGGCAAACTGAAGTAGGCCGCTTTGCTAAATCCTTCGGCAACGGTAGTAACCAAAACAGTGTTCAACCCAATGCTACGCTTAAGGGTTTAAAGTTAAGTGAACGTTCAACAACTGAGATTCAATTATTAGCTGAACAAGCCATCACTGTTAGCAAGCAAGAAGTGAAGGAGTTTACCTACAAAGTTGAATCGAATAAGCAATTTGAGGCTACGGTTAAGATTAAGGCAGATTTAAAAATTGATCCAACCAAGGCGATGAGCCATATAGAGAATATTTTTAAGGATGATGAAACTAAGAAAAAAGATGCTCAAAATTCTTTAACCATGAGTATGGGTCAAAATGAAGCTCTAGAAGCCACTTTTACATATAGTCCAGCTACTCAAGGCATCTTTGGCCGCGCTTCATTTGACCGTTTTACCAGTAACATCAAACTTAACACAAAGTTAAGAATTCAAGTTTCTAGTACGAGTGATCTAATGAAGAAGTTATTGGAGAATAGCTTAACCAGCAGTCTCAAAGACCAGAGCTTTGATAACGAAGGTGTTAACCTTTTCCCTTACACCTTATTTGCTTTGCTTTAA", "aa": "MLSQRILERWQTEVGRFAKSFGNGSNQNSVQPNATLKGLKLSERSTTEIQLLAEQAITVSKQEVKEFTYKVESNKQFEATVKIKADLKIDPTKAMSHIENIFKDDETKKKDAQNSLTMSMGQNEALEATFTYSPATQGIFGRASFDRFTSNIKLNTKLRIQVSSTSDLMKKLLENSLTSSLKDQSFDNEGVNLFPYTLFALL", "aa_hexdigest": "50c6448ff0fe4eb386c73cda3d2a38fd", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 765566, "stop": 766429, "observations": { "mutation": [ 766186, 766390, 766396 ], "stop": [ 766186, 766390, 766396 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75158", "query_cov": 1.0, "subject_cov": 0.7038327526132404, "identity": 0.995, "score": 374.0, "evalue": 2.73e-129, "gene_start": 1, "gene_end": 202, "reference_start": 86, "reference_end": 287, "reference_sequence": "MLGSMALKLRKWIWASIPSLALILSSCSALVASVELKSLSELQNLADRNTELTKNKRTLITTLRESYGLNPKGTLNLLFEGWRYTLLSQRILERWQTEVGRFAKSFGNGSNQNSVQPNATLKGLKLSERSTTEIQLLAEQAITVSKQEVKEFTYKVESNKQFEATVKIKADLKIDPTKAMSHIENIFKDDETKKKDAQNSLTMSMGQNEALEATFTYSPATQGIFGRASFDRFTSNIKLNTKLRIQVSSTSDLMKKLLENSLTSSLKDQSFDNEGVNLFPYTLFALL" }, "paralog": false, "identity": 0.9895470383275261, "score": 536.0, "evalue": 7.25e-196, "description": "Internal stop codon at 766186, 766390, 766396. Nonsense mutation around 766186, 766390, 766396.", "psc": { "uniref90_id": "UniRef90_P75158", "product": "Uncharacterized protein MPN_639", "uniref50_id": "UniRef50_P75158", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75158", "UniRef:UniRef50_P75158" ] }, "pscc": { "uniref50_id": "UniRef50_P75158", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75158" ], "product": "Uncharacterized protein MPN_639" } }, "id": "APKECPOBEN_1351", "locus": "GCA002090215_01351" }, { "type": "cds", "sequence": "contig_1", "start": 766437, "stop": 766865, "strand": "-", "frame": 2, "gene": null, "product": "putative lipo MG439-like protein", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P75150", "UniRef:UniRef90_P75157" ], "nt": "ATGGATGGAAAGATTGATGAATCAAGCAAAGCATTGATTAAATCATTCTTAGAGGGTAATAGTTCGGGAGGTAAAGGTAGCAATGGTAAAAACAGTATTGATGAAAGTGAATACACTGGTGAAAAAGCCAAATTCACTGGTAACTTTATTTTTACCTATACCCCACCAACTGGAGGTGCTAGAAACTTCTCTGATAAATCCTTTGATGTACCAACTAGTTCGATTAACTTTCCCGCTAATGTCAAGATTGATGTAACAACTTCTCACACAAAGCTGAATGAATTATTAGAAAGCAACGAACAGGTCAAAAAGATGAAAAGTCGTCAATTAACAGGTAAGTTATTTAATTTATTACCGTTTTTTACTACGCTCTGCTTTAATTCTTTTAGTCCTTTTACAGTATTTGCGGTAATTTTTACAATTGTTTAA", "aa": "MDGKIDESSKALIKSFLEGNSSGGKGSNGKNSIDESEYTGEKAKFTGNFIFTYTPPTGGARNFSDKSFDVPTSSINFPANVKIDVTTSHTKLNELLESNEQVKKMKSRQLTGKLFNLLPFFTTLCFNSFSPFTVFAVIFTIV", "aa_hexdigest": "020221207fcfa993ee9fa81708e7241d", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 766437, "stop": 767165, "observations": { "mutation": [ 766907, 767123 ], "stop": [ 766907, 767123 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75157", "query_cov": 1.0, "subject_cov": 0.47333333333333333, "identity": 1.0, "score": 270.0, "evalue": 2.13e-89, "gene_start": 1, "gene_end": 142, "reference_start": 159, "reference_end": 300, "reference_sequence": "MKLKLLLIPLLGSSLLLSACSSATSQVISSLSSAQKYFEANKGELNKKNVINILKDGYNSDPNKTVNALLAGWKYTLMDQKLLERNLDASRFASAFGSTSKKDDITPNISEKSLFLADTFPGISSEIAKVFKVEKQTVSGFSYSWNSPKKFQVNIQIKMDGKIDESSKALIKSFLEGNSSGGKGSNGKNSIDESEYTGEKAKFTGNFIFTYTPPTGGARNFSDKSFDVPTSSINFPANVKIDVTTSHTKLNELLESNEQVKKMKSRQLTGKLFNLLPFFTTLCFNSFSPFTVFAVIFTIV" }, "paralog": false, "identity": 0.9917355371900827, "score": 469.0, "evalue": 3.53e-170, "description": "Internal stop codon at 766907, 767123. Nonsense mutation around 766907, 767123.", "psc": { "uniref90_id": "UniRef90_P75157", "product": "Uncharacterized lipoprotein MG439 homolog 5", "uniref50_id": "UniRef50_P75150", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75157", "UniRef:UniRef50_P75150", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75150", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75150" ], "product": "Uncharacterized lipoprotein MG439 homolog 1" } }, "id": "APKECPOBEN_1352", "locus": "GCA002090215_01352" }, { "type": "cds", "sequence": "contig_1", "start": 766905, "stop": 767108, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00938.23" ], "nt": "ATGGATCAAAAGCTTTTAGAAAGAAATTTAGATGCTTCACGCTTTGCTAGCGCTTTTGGTTCTACTAGCAAGAAGGACGATATTACCCCTAACATTAGTGAAAAAAGTTTATTTTTAGCAGACACTTTTCCTGGTATTAGTTCAGAAATCGCTAAAGTATTTAAAGTTGAAAAGCAAACAGTTAGTGGTTTTTCTTACAGTTGA", "aa": "MDQKLLERNLDASRFASAFGSTSKKDDITPNISEKSLFLADTFPGISSEIAKVFKVEKQTVSGFSYS", "aa_hexdigest": "34706520f715a4c0fd24b91346f9bf69", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00938.23", "name": "Lipoprotein_3", "length": 68, "aa_cov": 1.0, "hmm_cov": 1.0, "evalue": 3.4856114743659758e-28, "score": 94.14722442626953, "start": 1, "stop": 67 } ], "seq_stats": { "molecular_weight": 7380.2125, "isoelectric_point": 6.140964317321777 }, "id": "APKECPOBEN_1353", "locus": "GCA002090215_01353" }, { "type": "cds", "sequence": "contig_1", "start": 767121, "stop": 767339, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00938.23" ], "nt": "ATGAAACTTAAACTGTTATTAATTCCTTTATTAGGATCTAGTTTATTGCTGAGCGCTTGTTCCTCTGCAACTAGCCAGGTTATATCGAGTTTAAGTAGCGCGCAAAAATACTTTGAAGCAAATAAAGGTGAATTGAACAAAAAGAACGTTATCAACATTCTGAAAGATGGCTATAACTCTGATCCTAACAAAACAGTTAACGCTCTTTTAGCAGGTTGA", "aa": "MKLKLLLIPLLGSSLLLSACSSATSQVISSLSSAQKYFEANKGELNKKNVINILKDGYNSDPNKTVNALLAG", "aa_hexdigest": "3be1533c2100bb96a1ce5416f96fd50b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00938.23", "name": "Lipoprotein_3", "length": 39, "aa_cov": 0.5277777777777778, "hmm_cov": 1.0, "evalue": 1.3889526444391616e-12, "score": 43.08498001098633, "start": 35, "stop": 72 } ], "seq_stats": { "molecular_weight": 7637.823099999997, "isoelectric_point": 9.46507091522217 }, "id": "APKECPOBEN_1354", "locus": "GCA002090215_01354" }, { "type": "cds", "sequence": "contig_1", "start": 767339, "stop": 768169, "strand": "-", "frame": 3, "gene": null, "product": "putative lipo MG440-like protein", "db_xrefs": [ "GO:0005886", "RefSeq:WP_010874998.1", "SO:0001217", "UniParc:UPI0000139815", "UniRef:UniRef100_P75156", "UniRef:UniRef50_P47678", "UniRef:UniRef90_P75156" ], "nt": "ATGAATAAGAAAAGACTATTACCCAAAGCTTCATTAGGTGCTTTATTCATGTTGTTCGGAACAGCTTTAACTGCTTGTTCTAATTCTGATTTTCAAACTAATTTAACAAGTTTGAATCAATTGAGGGAAGGCGTTAATCAAAATACCAGTCTAACCCAAGACAAGAAAGCTTTTGTGGAGTCACTGCAAAAAGCATTTGAAAATAATCCTGAAGGAACTACCAAAGTTTTATTAGACGCTTGGAAATTTACCTTATTAGATTCCAAAATTTTGGAGAGCAAAGACCCTTCTAGATTTGTCAAAGCTTTTGGCTCTGGAAAAAGTAATGAAGATGTTGAGCCTAATGCTAGTGTTAAAGGTTTAAGACTTGATAAACGATTTGAACCAAGCACTGCTAATATTATCAACAATGTTATTTCGCTAAATGAGCAAAAAGTAGAAGCATTTAATATCCAATACAAAAGTCGAACTAGTTTTAAAGTTCAAGTAAAACTTAATGCTCAAGGAAAATACCAAAAAAGTCAAGTTCAATCTTATCTACAACAAATTGGTTTAAATGATGGTGATTTGAAACAAGAAAGTGGCACTCTTTCTGCAGATCTTATTTTTACCTATACTGTACCTGAATCAAATCTTTTCTCTAGAAAGAACTTCGATACTTTAATGAAGAAAATTAATTTCAATACCACCTTAAAAATAGATATGGTTGGTAAAGACGAAATCATGAAAAAGATTCTTCAAAGTACTACCTTTACTAATAATTTGTCCAGTCAAACTTTCCAAGACCAATCAATTGATCTTTTACCTTACTTGCTTTATTCCATTCTCTAA", "aa": "MNKKRLLPKASLGALFMLFGTALTACSNSDFQTNLTSLNQLREGVNQNTSLTQDKKAFVESLQKAFENNPEGTTKVLLDAWKFTLLDSKILESKDPSRFVKAFGSGKSNEDVEPNASVKGLRLDKRFEPSTANIINNVISLNEQKVEAFNIQYKSRTSFKVQVKLNAQGKYQKSQVQSYLQQIGLNDGDLKQESGTLSADLIFTYTVPESNLFSRKNFDTLMKKINFNTTLKIDMVGKDEIMKKILQSTTFTNNLSSQTFQDQSIDLLPYLLYSIL", "aa_hexdigest": "4b9f41be117a2038f5733fcdea9871b4", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000139815", "ncbi_nrp_id": "WP_010874998.1", "uniref100_id": "UniRef100_P75156", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139815", "RefSeq:WP_010874998.1", "UniRef:UniRef100_P75156" ] }, "ips": { "uniref100_id": "UniRef100_P75156", "uniref90_id": "UniRef90_P75156" }, "psc": { "uniref90_id": "UniRef90_P75156", "product": "Uncharacterized lipoprotein MG440 homolog 3", "uniref50_id": "UniRef50_P47678", "go_ids": [ "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47678", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47678" ], "product": "Uncharacterized protein MG440" }, "genes": [], "id": "APKECPOBEN_1355", "locus": "GCA002090215_01355" }, { "type": "cds", "sequence": "contig_1", "start": 768172, "stop": 768537, "strand": "-", "frame": 1, "gene": null, "product": "putative lipo MG439-like protein", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P75150", "UniRef:UniRef90_P75155" ], "nt": "ATGGAAGGGATGATTAATGAAAAGAGTAAGGAAACAATTAAGTCTTTTTTGAGTAACAAAGATTCAAATGGAAGTAATAGCGTAGAAGAAAGTGAATATACTGGAGATAAAGCTAAATTCACTGCAGACTTTATCTTTTCCTATACTCCACCAACTGGAGGTACTAGATCTTTAACCGATAAATCATTTGATGTTATCACCAATATGATCAATTTCCCTGCTGATGTAAAGATTGATGTGAACACTTCGCATAATAAACTAAATGAACTGCTTGAGAAAAACGATCAAGTTAAGCGAATGAAAAGTCGTACATTTAATGGAAGAAATATTGACTTGCTACCCTTCTTTTACTATGCTCTACTTTAA", "aa": "MEGMINEKSKETIKSFLSNKDSNGSNSVEESEYTGDKAKFTADFIFSYTPPTGGTRSLTDKSFDVITNMINFPADVKIDVNTSHNKLNELLEKNDQVKRMKSRTFNGRNIDLLPFFYYALL", "aa_hexdigest": "0f6a0c56917a4bae56a36bb06b24bb2e", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 768172, "stop": 768836, "observations": { "deletion": [ 768608 ], "stop": [ 768578 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75155", "query_cov": 1.0, "subject_cov": 0.4336917562724014, "identity": 1.0, "score": 234.0, "evalue": 1.22e-75, "gene_start": 1, "gene_end": 121, "reference_start": 159, "reference_end": 279, "reference_sequence": "MKLKLYLIPLLASGIILSACSSTTSQVISSLSSAQKYFEANKGELNKNNVINILKDGYNSDPNKTVNALLAGWKYTLMDQKLLENNLDPSRFKNTFGPNKNKDDVTPNISEKGLFLNETFTGLGSQIAEVFNVQKQIVSGFSYSWTSPKSFKVNIHIKMEGMINEKSKETIKSFLSNKDSNGSNSVEESEYTGDKAKFTADFIFSYTPPTGGTRSLTDKSFDVITNMINFPADVKIDVNTSHNKLNELLEKNDQVKRMKSRTFNGRNIDLLPFFYYALL" }, "paralog": false, "identity": 0.995475113122172, "score": 436.0, "evalue": 1.82e-157, "description": "Internal stop codon at 768578. Frameshift due to deletion around 768608.", "psc": { "uniref90_id": "UniRef90_P75155", "product": "Uncharacterized lipoprotein MG439 homolog 4", "uniref50_id": "UniRef50_P75150", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75155", "UniRef:UniRef50_P75150", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75150", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75150" ], "product": "Uncharacterized lipoprotein MG439 homolog 1" } }, "id": "APKECPOBEN_1356", "locus": "GCA002090215_01356" }, { "type": "cds", "sequence": "contig_1", "start": 768552, "stop": 769010, "strand": "-", "frame": 2, "gene": null, "product": "putative lipo MG439-like protein", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P75150", "UniRef:UniRef90_P75155" ], "nt": "ATGAAACTTAAACTTTATTTAATTCCTCTTTTAGCTTCCGGCATTATTTTAAGTGCATGTTCTTCCACTACCAGCCAGGTTATATCGAGTTTAAGTAGCGCTCAAAAATACTTTGAAGCAAATAAAGGTGAGTTGAATAAAAATAACGTTATCAACATTCTGAAAGATGGCTATAACTCTGATCCTAACAAAACAGTTAACGCCTTACTAGCAGGCTGGAAGTACACCTTAATGGATCAAAAATTGTTGGAAAATAATTTAGATCCATCTCGTTTTAAAAATACTTTTGGCCCGAATAAAAATAAAGATGACGTTACTCCGAATATTAGCGAGAAAGGTTTATTTTTAAATGAAACTTTTACTGGTTTAGGAAGTCAAATTGCTGAAGTGTTTAATGTACAAAACAAATTGTTAGTGGTTTTTCTTACAGCTGAACCAGCCCTAAAAGTTTTAAAGTAA", "aa": "MKLKLYLIPLLASGIILSACSSTTSQVISSLSSAQKYFEANKGELNKNNVINILKDGYNSDPNKTVNALLAGWKYTLMDQKLLENNLDPSRFKNTFGPNKNKDDVTPNISEKGLFLNETFTGLGSQIAEVFNVQNKLLVVFLTAEPALKVLK", "aa_hexdigest": "83de4baeaa53114d936c1446bdd3bab5", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 768250, "stop": 769010, "observations": { "deletion": [ 768608 ], "stop": [ 768578 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75155", "query_cov": 0.9078947368421053, "subject_cov": 0.4946236559139785, "identity": 0.971, "score": 258.0, "evalue": 1.18e-84, "gene_start": 1, "gene_end": 138, "reference_start": 1, "reference_end": 138, "reference_sequence": "MKLKLYLIPLLASGIILSACSSTTSQVISSLSSAQKYFEANKGELNKNNVINILKDGYNSDPNKTVNALLAGWKYTLMDQKLLENNLDPSRFKNTFGPNKNKDDVTPNISEKGLFLNETFTGLGSQIAEVFNVQKQIVSGFSYSWTSPKSFKVNIHIKMEGMINEKSKETIKSFLSNKDSNGSNSVEESEYTGDKAKFTADFIFSYTPPTGGTRSLTDKSFDVITNMINFPADVKIDVNTSHNKLNELLEKNDQVKRMKSRTFNGRNIDLLPFFYYALL" }, "paralog": false, "identity": 0.9960474308300395, "score": 492.0, "evalue": 2.53e-179, "description": "Internal stop codon at 768578. Frameshift due to deletion around 768608.", "psc": { "uniref90_id": "UniRef90_P75155", "product": "Uncharacterized lipoprotein MG439 homolog 4", "uniref50_id": "UniRef50_P75150", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75155", "UniRef:UniRef50_P75150", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75150", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75150" ], "product": "Uncharacterized lipoprotein MG439 homolog 1" } }, "id": "APKECPOBEN_1357", "locus": "GCA002090215_01357" }, { "type": "cds", "sequence": "contig_1", "start": 769010, "stop": 769468, "strand": "-", "frame": 3, "gene": null, "product": "putative protein MPN_643", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47678", "UniRef:UniRef90_P75154" ], "nt": "GTGGAAAGATACACACAAGACACTGCCAATATAATTAATAACGTAATTAAACTGGACAAACAAAAAGTAGAAGCTTTTAGCATTCAATACAAAGATCCTAAAAACTTTAGAGTTCAAGTAAAAATTAATGCTAAAGGTAATTACAAAAAGGATACTGTAAAAACTTATTTAAGTCAAGTAGGTTTATCAGATGGTGATCTAAATGATACAGGTACATTAGAAGCTGAAATAATTTACACCTATATGCCACCTGCTGCCAGTTTCTTTTCTGCAAGTAAATTTGACAAACTAACAAGAGCCATAAACTTTAATACCAATTTACGAATACAAATTATAGGCAAAGATAGTGTGATGACTAAATTATTGCAACAAAGTAGTTTTGTAAAGCAACTAGCTGATCAAAAATTTCAAGACCAATCGATAAATCTCTTGCCTTACGTGCTTTACTCCATACTCTAA", "aa": "MERYTQDTANIINNVIKLDKQKVEAFSIQYKDPKNFRVQVKINAKGNYKKDTVKTYLSQVGLSDGDLNDTGTLEAEIIYTYMPPAASFFSASKFDKLTRAINFNTNLRIQIIGKDSVMTKLLQQSSFVKQLADQKFQDQSINLLPYVLYSIL", "aa_hexdigest": "9c3439f16ee6c689de554c12e96f4e75", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 769010, "stop": 769768, "observations": { "mutation": [ 769597 ], "stop": [ 769597 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75154", "query_cov": 1.0, "subject_cov": 0.5033112582781457, "identity": 0.993, "score": 290.0, "evalue": 4.3e-97, "gene_start": 1, "gene_end": 152, "reference_start": 151, "reference_end": 302, "reference_sequence": "MLKKLKVVRLLVNHLIYCPSIFMPYSKNMKKKIWNKTSLGALFMLFGTALTACSNSGFEANLTSLNQLRTSASKNTNLTQNKADLVTALKSAFENNPEGTTRVLLDAWKFTLLDAQILEKQDFSKFSKSFGSGRSIEDVEPSAGVRGLRLVERYTQDTANIINNVIKLDKQKVEAFSIQYKDPKNFRVQVKINAKGNYKKDTVKTYLSQVGLSDGDLNDTGTLEAEIIYTYMPPAASFFSASKFDKLTRAINFNTNLRIQIIGKDSVMTKLLQQSSFVKQLADQKFQDQSINLLPYVLYSIL" }, "paralog": false, "identity": 0.996031746031746, "score": 484.0, "evalue": 9.2e-176, "description": "Internal stop codon at 769597. Nonsense mutation around 769597.", "psc": { "uniref90_id": "UniRef90_P75154", "product": "Uncharacterized protein MPN_643", "uniref50_id": "UniRef50_P47678", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75154", "UniRef:UniRef50_P47678" ] }, "pscc": { "uniref50_id": "UniRef50_P47678", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47678" ], "product": "Uncharacterized protein MG440" } }, "id": "APKECPOBEN_1358", "locus": "GCA002090215_01358" }, { "type": "cds", "sequence": "contig_1", "start": 769595, "stop": 769789, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00938.23" ], "nt": "ATGTTGTTTGGAACAGCTTTAACTGCTTGTTCTAATTCTGGTTTTGAAGCAAATCTTACTAGCTTGAATCAATTAAGAACCAGTGCAAGTAAAAATACTAATTTAACCCAAAATAAAGCTGATTTAGTTACAGCCTTAAAATCAGCTTTTGAAAATAATCCAGAAGGCACTACTAGGGTTTTATTAGACGCTTGA", "aa": "MLFGTALTACSNSGFEANLTSLNQLRTSASKNTNLTQNKADLVTALKSAFENNPEGTTRVLLDA", "aa_hexdigest": "1f85c5aa94dd5ce0d870cf36554211e7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00938.23", "name": "Lipoprotein_3", "length": 39, "aa_cov": 0.609375, "hmm_cov": 1.0, "evalue": 6.263023218203884e-11, "score": 37.6708984375, "start": 26, "stop": 64 } ], "seq_stats": { "molecular_weight": 6776.489100000002, "isoelectric_point": 6.036323738098144 }, "id": "APKECPOBEN_1359", "locus": "GCA002090215_01359" }, { "type": "cds", "sequence": "contig_1", "start": 769839, "stop": 770201, "strand": "-", "frame": 2, "gene": null, "product": "putative lipo MG439-like protein", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P75150", "UniRef:UniRef90_P75153" ], "nt": "ATGATGCAAGGCATTGTTGGCAGTGGTGAAGAAAGTAATTCTTTAATTAAATCTTTCTTGAGCTCTGGAAATAATGGCAATGTTACTGAAAATGATTTTAATAATGGAAATGCAAACTTTGATGGCACATTCATATTCACTTTTACTCCGCCTACAGATGGAAGAAGATTTGCATTTAGTAACTTTGATCCAATCACTGGAACAATTAATTTCCCTGCTAATCTTCAAATTGATGCAAGCACAACTCATGAAAAACTCAATATCCTAATGCAAAACAACGAACATGTTAAAAAAATTAAAAGTCGTTCGTTTACTGGTAAATCATTTGATCTATTGCCCTTCTATTTTTATGCCTTACTCTAA", "aa": "MMQGIVGSGEESNSLIKSFLSSGNNGNVTENDFNNGNANFDGTFIFTFTPPTDGRRFAFSNFDPITGTINFPANLQIDASTTHEKLNILMQNNEHVKKIKSRSFTGKSFDLLPFYFYALL", "aa_hexdigest": "fe00e36e5c8379a0bf758de99fde893b", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 769839, "stop": 770501, "observations": { "mutation": [ 770240, 770456 ], "stop": [ 770240, 770456 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75153", "query_cov": 1.0, "subject_cov": 0.42402826855123676, "identity": 1.0, "score": 236.0, "evalue": 1.15e-76, "gene_start": 1, "gene_end": 120, "reference_start": 164, "reference_end": 283, "reference_sequence": "MNKKRLLFRTPLDALFLLFGTALSACSSTATNVISSLSSAQKYFDAHKSELIKKNVINLLKEGYSTDSKATVNSLFAGWKYTLMDQKILERNLDASRFTKAFGTNKGKDDVIPSISEKGLFLDETYSGVSQQIAKVLGVQSQKVTGFSYSWSSTTNFKVVISFMMQGIVGSGEESNSLIKSFLSSGNNGNVTENDFNNGNANFDGTFIFTFTPPTDGRRFAFSNFDPITGTINFPANLQIDASTTHEKLNILMQNNEHVKKIKSRSFTGKSFDLLPFYFYALL" }, "paralog": false, "identity": 0.990909090909091, "score": 430.0, "evalue": 2.72e-155, "description": "Internal stop codon at 770240, 770456. Nonsense mutation around 770240, 770456.", "psc": { "uniref90_id": "UniRef90_P75153", "product": "Uncharacterized lipoprotein MG439 homolog 3", "uniref50_id": "UniRef50_P75150", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75153", "UniRef:UniRef50_P75150", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75150", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75150" ], "product": "Uncharacterized lipoprotein MG439 homolog 1" } }, "id": "APKECPOBEN_1360", "locus": "GCA002090215_01360" }, { "type": "cds", "sequence": "contig_1", "start": 770238, "stop": 770441, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00938.23" ], "nt": "ATGGATCAAAAAATACTGGAAAGAAATCTAGATGCTAGTCGCTTTACAAAAGCATTTGGTACTAACAAAGGCAAAGATGATGTCATTCCAAGTATTAGTGAGAAAGGTTTATTTTTAGATGAAACTTACAGTGGTGTAAGTCAACAAATAGCTAAAGTACTTGGAGTGCAAAGCCAAAAAGTAACTGGATTTTCTTACAGCTGA", "aa": "MDQKILERNLDASRFTKAFGTNKGKDDVIPSISEKGLFLDETYSGVSQQIAKVLGVQSQKVTGFSYS", "aa_hexdigest": "4cdc6e1d98c7e3e755b2d743e9930c32", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00938.23", "name": "Lipoprotein_3", "length": 68, "aa_cov": 1.0, "hmm_cov": 1.0, "evalue": 2.1828087930373014e-30, "score": 101.35880279541016, "start": 1, "stop": 67 } ], "seq_stats": { "molecular_weight": 7373.2247000000025, "isoelectric_point": 8.007634162902832 }, "id": "APKECPOBEN_1361", "locus": "GCA002090215_01361" }, { "type": "cds", "sequence": "contig_1", "start": 770454, "stop": 770690, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00938.23" ], "nt": "ATGAATAAGAAAAGGCTATTATTCAGAACTCCATTAGATGCTTTATTTTTATTGTTCGGCACTGCTTTAAGTGCCTGTTCATCAACTGCCACCAATGTTATTTCTAGTTTAAGTAGTGCGCAAAAATACTTTGATGCTCACAAAAGTGAACTAATCAAAAAGAATGTAATTAATCTTTTAAAAGAAGGTTACAGTACTGATTCAAAAGCTACAGTTAATTCACTTTTTGCTGGTTGA", "aa": "MNKKRLLFRTPLDALFLLFGTALSACSSTATNVISSLSSAQKYFDAHKSELIKKNVINLLKEGYSTDSKATVNSLFAG", "aa_hexdigest": "a27f1dd6ef62585b245e844e16b32470", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00938.23", "name": "Lipoprotein_3", "length": 39, "aa_cov": 0.48717948717948717, "hmm_cov": 1.0, "evalue": 5.695667854164809e-10, "score": 34.532745361328125, "start": 41, "stop": 78 } ], "seq_stats": { "molecular_weight": 8487.714099999994, "isoelectric_point": 9.666728019714355 }, "id": "APKECPOBEN_1362", "locus": "GCA002090215_01362" }, { "type": "cds", "sequence": "contig_1", "start": 770693, "stop": 771067, "strand": "-", "frame": 3, "gene": null, "product": "putative lipo MG439-like protein", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P75150", "UniRef:UniRef90_P75152" ], "nt": "ATGAAGGGAAAAGTTGGAAGTGATTCAGAGAGTCAACAATTAATTAAATCTTTTTTAAGTTCTGATAACAATGGATCAAATCAAAATGGCGGAGTAAAAGAAACTGACTTTAATGGTGATTCTGCTAATTTCGATGGTTTCTTTACTTTTACTTATACACCTCCTACTCAAAGTAGAAAATTTGGTGCCACTTCTTTTGACCCTCTTACAACTAAGATTAACTTTCCAGCAGACTTACAAATAGATGTAAGCACTACTCACCAAAAGTTAAATACTTTAATGGAAGCCAATGAACAAGTAAAACAGATTAAAAGCCGTAAGTTTACTGGTAAAACCTTTGACTTGTTACCGTTCTTTTACTACGCACTACTTTAA", "aa": "MKGKVGSDSESQQLIKSFLSSDNNGSNQNGGVKETDFNGDSANFDGFFTFTYTPPTQSRKFGATSFDPLTTKINFPADLQIDVSTTHQKLNTLMEANEQVKQIKSRKFTGKTFDLLPFFYYALL", "aa_hexdigest": "418b1dd1c030be37cf8acbe8ca0300f6", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 770693, "stop": 771367, "observations": { "mutation": [ 771109, 771322 ], "stop": [ 771109, 771322 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75152", "query_cov": 1.0, "subject_cov": 0.4381625441696113, "identity": 1.0, "score": 241.0, "evalue": 2.91e-78, "gene_start": 1, "gene_end": 124, "reference_start": 160, "reference_end": 283, "reference_sequence": "MKLKLKFLLISLLGSSLLLSACSSAATQVISSLSSAQKYFESSQGELNKKNVIKILKEGYESDANKAVHALLAGWKYTLMDQQLLSKEVDSRFIKAFGSGRDKGDVTPSVSEKGLYLNETYTGFSSQIAKVLGVQSQTVKQFNYKWSSNSDFKVQIQISMKGKVGSDSESQQLIKSFLSSDNNGSNQNGGVKETDFNGDSANFDGFFTFTYTPPTQSRKFGATSFDPLTTKINFPADLQIDVSTTHQKLNTLMEANEQVKQIKSRKFTGKTFDLLPFFYYALL" }, "paralog": false, "identity": 0.9910714285714286, "score": 437.0, "evalue": 5.79e-158, "description": "Internal stop codon at 771109, 771322. Nonsense mutation around 771109, 771322.", "psc": { "uniref90_id": "UniRef90_P75152", "product": "Uncharacterized lipoprotein MG439 homolog 2", "uniref50_id": "UniRef50_P75150", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75152", "UniRef:UniRef50_P75150", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75150", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75150" ], "product": "Uncharacterized lipoprotein MG439 homolog 1" } }, "id": "APKECPOBEN_1363", "locus": "GCA002090215_01363" }, { "type": "cds", "sequence": "contig_1", "start": 771107, "stop": 771307, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00938.23" ], "nt": "ATGGACCAGCAATTACTTTCTAAAGAAGTTGATAGTCGCTTTATCAAAGCTTTTGGTAGCGGACGCGATAAAGGCGATGTAACTCCTAGTGTTAGTGAAAAGGGATTGTACTTAAACGAAACTTACACTGGCTTTAGTAGTCAAATTGCCAAAGTCTTAGGTGTACAAAGTCAAACTGTTAAACAATTTAATTACAAGTGA", "aa": "MDQQLLSKEVDSRFIKAFGSGRDKGDVTPSVSEKGLYLNETYTGFSSQIAKVLGVQSQTVKQFNYK", "aa_hexdigest": "6b02599e548b92439bb16ad32249be61", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00938.23", "name": "Lipoprotein_3", "length": 68, "aa_cov": 1.0, "hmm_cov": 1.0, "evalue": 9.281227347917318e-28, "score": 92.75507354736328, "start": 1, "stop": 66 } ], "seq_stats": { "molecular_weight": 7351.177600000002, "isoelectric_point": 9.04899196624756 }, "id": "APKECPOBEN_1364", "locus": "GCA002090215_01364" }, { "type": "cds", "sequence": "contig_1", "start": 771320, "stop": 771544, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00938.23" ], "nt": "ATGAAACTGAAACTTAAATTTCTATTAATTTCTCTTTTAGGTTCTAGTTTGTTGTTAAGCGCTTGTTCTTCAGCAGCTACTCAAGTAATTTCTAGTTTAAGTAGTGCTCAAAAATACTTTGAATCCAGTCAAGGTGAACTCAACAAAAAGAATGTCATCAAAATCTTAAAGGAAGGTTATGAATCTGATGCTAATAAGGCAGTCCATGCTTTGCTAGCTGGTTGA", "aa": "MKLKLKFLLISLLGSSLLLSACSSAATQVISSLSSAQKYFESSQGELNKKNVIKILKEGYESDANKAVHALLAG", "aa_hexdigest": "8109af68c9dce6c3800d644fce144112", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00938.23", "name": "Lipoprotein_3", "length": 39, "aa_cov": 0.5135135135135135, "hmm_cov": 1.0, "evalue": 2.511131236203525e-10, "score": 35.69691848754883, "start": 37, "stop": 74 } ], "seq_stats": { "molecular_weight": 7886.143999999996, "isoelectric_point": 9.41762218475342 }, "id": "APKECPOBEN_1365", "locus": "GCA002090215_01365" }, { "type": "cds", "sequence": "contig_1", "start": 771544, "stop": 772107, "strand": "-", "frame": 1, "gene": null, "product": "putative lipo MG440-like protein", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P47678", "UniRef:UniRef90_P75151" ], "nt": "TTGGAAAAACAAGATCCAGTTAAATTTGTTAAAGCATTTGGTAGCGGTAAAAGTAAGGAAGACATAGAACCTAGTGCTGGCGTACGTGGTTTAAGATTTGTTGAACGTTTTGGTGATAACACAGCTAGTCTTATTAAAAATGCAATTTTATTGGATCAACAAAAAGTAGAGGTTTTTAATATTCGCTTTAAAAGTAGTCGTGATTTCACTATTCAGATTAAGCTGAATGCAAAAGGTAATTACAAAAAGAGTGAAGTCGAGAAATATAAGTCACAAATTGGTGTTCAAGATAATGAACTGGGCGATAAGGAAGAAGGCACTTTAGAAGCTGATTTAATCTTCACTTACACTCCACCTGCTTCAAATTTGTTTTCTAAGAGTAATTTTGACAAGTTAACTAAATCGATTAATTTCAATACAAATCTGAAATTAGAAATGGTTGGTAAAGACGAAGTAATGAGAAAGATTCTTAACAGTAGTGCCTTCACTAATAATCTTGCCAGTGTTAATTACCCAAACCAAGCAGTTAATTTACTGCCTTACGTACTTTACTCCATCCTCTAA", "aa": "MEKQDPVKFVKAFGSGKSKEDIEPSAGVRGLRFVERFGDNTASLIKNAILLDQQKVEVFNIRFKSSRDFTIQIKLNAKGNYKKSEVEKYKSQIGVQDNELGDKEEGTLEADLIFTYTPPASNLFSKSNFDKLTKSINFNTNLKLEMVGKDEVMRKILNSSAFTNNLASVNYPNQAVNLLPYVLYSIL", "aa_hexdigest": "c49e5b47447712b59840004c9c12d823", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 771544, "stop": 772377, "observations": { "mutation": [ 772137, 772359 ], "stop": [ 772137, 772359 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75151", "query_cov": 1.0, "subject_cov": 0.6750902527075813, "identity": 0.995, "score": 357.0, "evalue": 2.6e-123, "gene_start": 1, "gene_end": 187, "reference_start": 91, "reference_end": 277, "reference_sequence": "MNKKSIWSKTAFGSLFLLLGTAFTACSNYDFQANLTSLNQLRTSANKDINLTQDKNTLIDALKTAFENNSEGTTKVLLDAWKFSLYDAKILEKQDPVKFVKAFGSGKSKEDIEPSAGVRGLRFVERFGDNTASLIKNAILLDQQKVEVFNIRFKSSRDFTIQIKLNAKGNYKKSEVEKYKSQIGVQDNELGDKEEGTLEADLIFTYTPPASNLFSKSNFDKLTKSINFNTNLKLEMVGKDEVMRKILNSSAFTNNLASVNYPNQAVNLLPYVLYSIL" }, "paralog": false, "identity": 0.9927797833935018, "score": 531.0, "evalue": 3.81e-194, "description": "Internal stop codon at 772137, 772359. Nonsense mutation around 772137, 772359.", "psc": { "uniref90_id": "UniRef90_P75151", "product": "Uncharacterized lipoprotein MG440 homolog 1", "uniref50_id": "UniRef50_P47678", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75151", "UniRef:UniRef50_P47678", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47678", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47678" ], "product": "Uncharacterized protein MG440" } }, "id": "APKECPOBEN_1366", "locus": "GCA002090215_01366" }, { "type": "cds", "sequence": "contig_1", "start": 772135, "stop": 772335, "strand": "-", "frame": 1, "gene": null, "product": "lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001912A3C4", "UniRef:UniRef90_UPI001912A3C4" ], "nt": "TTGTTTCTTTTATTAGGAACAGCGTTTACTGCTTGTTCTAACTATGACTTTCAAGCTAACTTAACTAGTTTGAACCAATTGAGAACTTCAGCCAATAAAGACATTAATTTAACTCAGGACAAAAATACCTTAATTGATGCTTTAAAAACTGCCTTTGAAAATAATTCAGAAGGTACCACTAAGGTTTTACTAGATGCCTGA", "aa": "MFLLLGTAFTACSNYDFQANLTSLNQLRTSANKDINLTQDKNTLIDALKTAFENNSEGTTKVLLDA", "aa_hexdigest": "c535947d0bfa283cab62c1a492dee459", "start_type": "TTG", "rbs_motif": "AAAA", "genes": [], "truncated": "both", "pseudogene": { "start": 772057, "stop": 772377, "observations": { "mutation": [ 772137, 772359 ], "stop": [ 772137, 772359 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001912A3C4", "query_cov": 1.0, "subject_cov": 0.6226415094339622, "identity": 0.985, "score": 124.0, "evalue": 1.27e-35, "gene_start": 1, "gene_end": 66, "reference_start": 15, "reference_end": 80, "reference_sequence": "MNKKSIWSKTAFGSLFLLLGTAFTACSNYDFQANLTSLNQLRTSANKDINLTQDKNTLIDALKTAFENNSEGTTKVLLDAWKFSLYDAKILEKQDRVKFVKAFGRG" }, "paralog": false, "identity": 0.9622641509433962, "score": 196.0, "evalue": 2.95e-66, "description": "Internal stop codon at 772137, 772359. Nonsense mutation around 772137, 772359.", "psc": { "uniref90_id": "UniRef90_UPI001912A3C4", "product": "lipoprotein", "uniref50_id": "UniRef50_UPI001912A3C4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001912A3C4", "UniRef:UniRef50_UPI001912A3C4" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001912A3C4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001912A3C4" ], "product": "lipoprotein" } }, "id": "APKECPOBEN_1367", "locus": "GCA002090215_01367" }, { "type": "cds", "sequence": "contig_1", "start": 772400, "stop": 772783, "strand": "-", "frame": 3, "gene": null, "product": "putative lipo MG439-like protein", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P75150", "UniRef:UniRef90_P75150" ], "nt": "ATGAAAGGAAAAGTTGGTAGTGATGGCACAAGTCAAACTTTAATTAAGTCTTTTTTAGGTACTGATAGCAAAGGCAGTGGAAGCAATAATCAAAATGGTGGTGTAACTGAAAAAGATTTTGAAGGTGACCAAGCTAACTTTGATGGCAATTTTATCTTTACTTACACTCAACCTTCCGATGGCAGAAGACTTGCGTCCAACAACTTTGATCCAATTACTGGAACCATCAATTTTCCTGCTGATCTCCAAATTGAGGTAAGTACTAGTCATGAAAAGTTAAACACTTTAATGACAACTAATACTCAGGTAGGAATGATTAAGAACCGCTCTTTTAAAGGCAAATCATTTAATCTTTTGCCTTTCTTTTACTACGCCCTTCTCTAA", "aa": "MKGKVGSDGTSQTLIKSFLGTDSKGSGSNNQNGGVTEKDFEGDQANFDGNFIFTYTQPSDGRRLASNNFDPITGTINFPADLQIEVSTSHEKLNTLMTTNTQVGMIKNRSFKGKSFNLLPFFYYALL", "aa_hexdigest": "71a8e5688db1ea025521316d9b1a0062", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 772393, "stop": 773083, "observations": { "insertion": [ 772420 ], "mutation": [ 772825, 773038 ], "stop": [ 772825, 773038 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75150", "query_cov": 0.9606299212598425, "subject_cov": 0.4178082191780822, "identity": 0.992, "score": 238.0, "evalue": 7.14e-77, "gene_start": 1, "gene_end": 122, "reference_start": 164, "reference_end": 285, "reference_sequence": "MNKKSILSKTSLGSLFFLFGTALSACSSATTEVISSFSSAQKYFSANKKELNKRNLVTILKDSYNSDPKSTVNSLLAGWKYSLLDQKLLENPMDPSRFSKAFGSNTKDDVTPNISEKGLYLAETYPGVSSQIAQVLGVQSQKVTGFSYSWTSKTKFEVKILIKMKGKVGSDGTSQTLIKSFLGTDSKGSGSNNQNGGVTEKDFEGDQANFDGNFIFTYTQPSDGRRLASSNFDPITGTINFPADLQIEVSTSHEKLNTLMTTNTQVGMIKNRSFKGKSFNLLPFFTTPFSNF" }, "paralog": false, "identity": 0.982532751091703, "score": 440.0, "evalue": 5.44e-159, "description": "Internal stop codon at 772825, 773038. Frameshift due to insertion around 772420. Nonsense mutation around 772825, 773038.", "psc": { "uniref90_id": "UniRef90_P75150", "product": "Uncharacterized lipoprotein MG439 homolog 1", "uniref50_id": "UniRef50_P75150", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75150", "UniRef:UniRef50_P75150", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75150", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75150" ], "product": "Uncharacterized lipoprotein MG439 homolog 1" } }, "id": "APKECPOBEN_1368", "locus": "GCA002090215_01368" }, { "type": "cds", "sequence": "contig_1", "start": 772823, "stop": 773026, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00938.23" ], "nt": "TTGCTGGATCAAAAGTTATTGGAGAACCCAATGGATCCTTCCCGTTTTTCTAAAGCTTTTGGTTCTAACACTAAAGATGATGTTAACCCGAACATCAGTGAAAAAGGTTTATATCTTGCAGAAACTTATCCCGGTGTAAGTAGTCAAATCGCTCAAGTTCTTGGTGTCCAAAGCCAAAAGGTAACTGGTTTTTCTTATAGCTGA", "aa": "MLDQKLLENPMDPSRFSKAFGSNTKDDVNPNISEKGLYLAETYPGVSSQIAQVLGVQSQKVTGFSYS", "aa_hexdigest": "c61772bc211af54c92902807b3142935", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00938.23", "name": "Lipoprotein_3", "length": 69, "aa_cov": 1.0, "hmm_cov": 1.0, "evalue": 2.0339270206650776e-29, "score": 98.18608856201172, "start": 1, "stop": 67 } ], "seq_stats": { "molecular_weight": 7328.1177000000025, "isoelectric_point": 5.042607688903809 }, "id": "APKECPOBEN_1369", "locus": "GCA002090215_01369" }, { "type": "cds", "sequence": "contig_1", "start": 773036, "stop": 773272, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00938.23" ], "nt": "ATGAACAAAAAATCGATTTTGTCAAAGACCAGTCTAGGTTCTTTGTTTTTCCTGTTTGGCACAGCTTTAAGTGCCTGCTCCTCTGCCACCACAGAAGTAATTTCCAGTTTTAGTAGTGCCCAGAAGTACTTTAGTGCCAACAAAAAAGAACTTAATAAACGTAATCTAGTAACCATTTTGAAGGATAGTTATAATTCTGATCCCAAGTCAACAGTTAATTCTTTATTAGCTGGCTGA", "aa": "MNKKSILSKTSLGSLFFLFGTALSACSSATTEVISSFSSAQKYFSANKKELNKRNLVTILKDSYNSDPKSTVNSLLAG", "aa_hexdigest": "40b50d430d2f5d5994d1915b01417aac", "start_type": "ATG", "rbs_motif": "AAAA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00938.23", "name": "Lipoprotein_3", "length": 39, "aa_cov": 0.48717948717948717, "hmm_cov": 1.0, "evalue": 5.831186258809286e-12, "score": 41.045589447021484, "start": 41, "stop": 78 } ], "seq_stats": { "molecular_weight": 8388.492099999996, "isoelectric_point": 9.78032131195068 }, "id": "APKECPOBEN_1370", "locus": "GCA002090215_01370" }, { "type": "cds", "sequence": "contig_1", "start": 773629, "stop": 773793, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAGCCAATTTAAAGCAAATGTACTAGCTATCTTACTAGTCTCCTTGTTTTTAATTAACGGTCTTGTTTTTCTTTCGAAAACATTATTTAAGTTATTTAATTTTCGTGTCACCCACTCATTAAATAACTACTATACCTTTAATCTGTTAATTAAAGCTTGA", "aa": "MKSQFKANVLAILLVSLFLINGLVFLSKTLFKLFNFRVTHSLNNYYTFNLLIKA", "aa_hexdigest": "7b062f5e42e9a4aca979e4c01b7d1285", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6268.4995, "isoelectric_point": 10.390772438049318 }, "id": "APKECPOBEN_1371", "locus": "GCA002090215_01371" }, { "type": "cds", "sequence": "contig_1", "start": 774680, "stop": 775090, "strand": "-", "frame": 3, "gene": null, "product": "putative protein MPN_649", "db_xrefs": [ "RefSeq:WP_010875006.1", "SO:0001217", "UniParc:UPI000013AF9D", "UniRef:UniRef100_P75148", "UniRef:UniRef50_P75148", "UniRef:UniRef90_P75148" ], "nt": "TTGAAAATTTCCTTAAGCACTAGTCTCTTTAGTATCGAACAAAAGGTTGAGTATTTCAATTTGAATTACCAAAGCCTTAGTGATTTTTCAGTTGTTGCCAAATTGAACTATACCTTTACATGGTATGGCAATGATTTTTCAATAGGTTTCGCTCCAAAAAAAGGTGAAAAGCTTTATTTTGATCTCTTTTTTACTTTTAAAGCTTCTCCCAACCATCCATTTGCAGCAGAAAACTTTAAACCTGATTCAGAAGCAATCGACTTTTATGTTTCATTTAGCTGGAGATTAGAAGGAAAAGACGAAGTTAGCAAAAAACTTTTTGAATTAAGTGTTTTTGGTAGAGCAAGAGCTTTTCAAATTGATGACTACAAAATCAACCTTTTTAGTTATTTAGTTTACGTAATTAGATAA", "aa": "MKISLSTSLFSIEQKVEYFNLNYQSLSDFSVVAKLNYTFTWYGNDFSIGFAPKKGEKLYFDLFFTFKASPNHPFAAENFKPDSEAIDFYVSFSWRLEGKDEVSKKLFELSVFGRARAFQIDDYKINLFSYLVYVIR", "aa_hexdigest": "74f3664669cc55e687dbc7fd4ab2c752", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013AF9D", "ncbi_nrp_id": "WP_010875006.1", "uniref100_id": "UniRef100_P75148", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013AF9D", "RefSeq:WP_010875006.1", "UniRef:UniRef100_P75148" ] }, "ips": { "uniref100_id": "UniRef100_P75148", "uniref90_id": "UniRef90_P75148" }, "psc": { "uniref90_id": "UniRef90_P75148", "product": "Uncharacterized protein MPN_649", "uniref50_id": "UniRef50_P75148" }, "pscc": { "uniref50_id": "UniRef50_P75148", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75148" ], "product": "Uncharacterized protein MPN_649" }, "genes": [], "id": "APKECPOBEN_1372", "locus": "GCA002090215_01372" }, { "type": "cds", "sequence": "contig_1", "start": 775096, "stop": 775380, "strand": "-", "frame": 1, "gene": null, "product": "putative lipoprotein MPN_650", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P75147", "UniRef:UniRef90_P75147" ], "nt": "ATGAAATTTAAATATCTATCAACTCCGTTATTATTTTCAGCTTTATTATTTAGCGCTTGTTCTAGCGTCAATGTTGGAGCTAATTTGAAAAGTCTTATTAAGGAAACCACCGATCAAGACCTTGATTTGTCTGAATCAGTTAGTACTTCTGAGGGAAAGAAGAATTTAATAAGTTCTTTAAAGAAGTCTTACGAAACTAACCCTAGCAAAACAGCTAGTGTACTCTTGAATGCCTGGAAGCAAAGTGTAGAAAACGGTCAAATAGAGAGTTCTGAAACTAATTGA", "aa": "MKFKYLSTPLLFSALLFSACSSVNVGANLKSLIKETTDQDLDLSESVSTSEGKKNLISSLKKSYETNPSKTASVLLNAWKQSVENGQIESSETN", "aa_hexdigest": "856656c27a4a32982dfc34ad2685c8b5", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75147", "query_cov": 1.0, "subject_cov": 0.9306930693069307, "identity": 1.0, "score": 167.0, "evalue": 2.92e-52, "valid": true, "product": "Uncharacterized lipoprotein MPN_650", "uniref50_id": "UniRef50_P75147", "go_ids": [ "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75147", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75147" ], "product": "Uncharacterized lipoprotein MPN_650" }, "genes": [], "id": "APKECPOBEN_1373", "locus": "GCA002090215_01373" }, { "type": "cds", "sequence": "contig_1", "start": 775380, "stop": 775517, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W7G3", "UniRef:UniRef90_A0AB38W7G3" ], "nt": "ATGAGACTAGAAGTTTTTCCTAAGGATGAAATAACCAAAAAACTTTTAGATAATGGTTCTATTGGAAAAGTGGTAGATAAAATTTACTTTGATTACAAAGTAAATTTACTGCTATATCTGGTTTATTCAGTCCTCTAA", "aa": "MRLEVFPKDEITKKLLDNGSIGKVVDKIYFDYKVNLLLYLVYSVL", "aa_hexdigest": "3c3334c3be7335e5acc8c45bae3e2fbd", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0AB38W7G3", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.978, "score": 89.7, "evalue": 5.35e-23, "valid": true, "uniref50_id": "UniRef50_A0AB38W7G3" }, "pscc": { "uniref50_id": "UniRef50_A0AB38W7G3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W7G3" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5294.249900000001, "isoelectric_point": 7.982878303527833 }, "id": "APKECPOBEN_1374", "locus": "GCA002090215_01374" }, { "type": "cds", "sequence": "contig_1", "start": 775542, "stop": 775775, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W5Y3", "UniRef:UniRef90_A0AB38W5Y3" ], "nt": "TTGTTTGACATTCAGGCAAACAATAAAAATGATTTTAAGGTTGTTGTTAAAATGAACACAACTTTTAATGCTGATATTAATCAAAAACCAAATCAAGGAGGGTTAATAATGTCGGGCTTTGGCAACTACTTGCTTAATTTTGATCTTATTTACACATACAAACCATTTAAAAACAAACCAATTCTAGAGACAAGTATTGTTAAAAACTCTGATAATAAATTAGGGAATTTGTGA", "aa": "MFDIQANNKNDFKVVVKMNTTFNADINQKPNQGGLIMSGFGNYLLNFDLIYTYKPFKNKPILETSIVKNSDNKLGNL", "aa_hexdigest": "639af4a3159d0034284dc2ca5fc6b675", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0AB38W5Y3", "query_cov": 1.0, "subject_cov": 0.927710843373494, "identity": 1.0, "score": 156.0, "evalue": 2.21e-48, "valid": true, "uniref50_id": "UniRef50_A0AB38W5Y3" }, "pscc": { "uniref50_id": "UniRef50_A0AB38W5Y3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W5Y3" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8761.966999999995, "isoelectric_point": 9.302352714538571 }, "id": "APKECPOBEN_1375", "locus": "GCA002090215_01375" }, { "type": "cds", "sequence": "contig_1", "start": 776294, "stop": 776446, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E56736E", "UniRef:UniRef90_UPI001E56736E" ], "nt": "GTGATTGCTGTCATTATTGCCATTGCTGTAATAGCTGCTGGTCAAACCGAAGCATTTACAAAAATTGTTGGTCAAAAATCAGTAATGTTTTTAGGTGTGATGATATTCGGCCCAATTGCAGCGCTAATCTTAAAACACACTGAAAAGTTTTGA", "aa": "MIAVIIAIAVIAAGQTEAFTKIVGQKSVMFLGVMIFGPIAALILKHTEKF", "aa_hexdigest": "bc86010e07d4734418fd8b24215049f8", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 776294, "stop": 776506, "observations": { "mutation": [ 776444 ], "stop": [ 776444 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E56736E", "query_cov": 1.0, "subject_cov": 0.7142857142857143, "identity": 1.0, "score": 91.3, "evalue": 3.01e-23, "gene_start": 1, "gene_end": 50, "reference_start": 1, "reference_end": 50, "reference_sequence": "MIAVIIAIAVIAAGQTEAFTKIVGQKSVMFLGVMIFGPIAALILKHTEKFWIHRIKSGFEMLVNNFYLAF" }, "paralog": false, "identity": 0.9571428571428572, "score": 129.0, "evalue": 8.24e-41, "description": "Internal stop codon at 776444. Nonsense mutation around 776444.", "psc": { "uniref90_id": "UniRef90_UPI001E56736E", "uniref50_id": "UniRef50_UPI001E56736E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E56736E", "UniRef:UniRef50_UPI001E56736E" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E56736E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E56736E" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 5302.492199999998, "isoelectric_point": 9.52476863861084 }, "id": "APKECPOBEN_1376", "locus": "GCA002090215_01376" }, { "type": "cds", "sequence": "contig_1", "start": 776474, "stop": 777517, "strand": "+", "frame": 2, "gene": null, "product": "PTS mannitol transporter subunit IICB", "db_xrefs": [ "EC:2.7.1.197", "KEGG:K02800", "SO:0001217", "UniRef:UniRef50_UPI001F2174FA", "UniRef:UniRef90_UPI001F2174FA" ], "nt": "ATGCTGGTAAATAATTTTTACCTTGGCTTTTTAGGTTTCGCCTTAATATTTCCTAGTTTTTACCTATCAATTTACTTAATTGGTTACATTCAATTAGGTTTAAAACTGCTTGTTGAAATAATGCAACAGTACAAACTTTATCCAATAGCTGCCATTGTGATAGAGCCGGCCAAAGTTTTATTCCTAAATAATGCCATTAACCATGGCGTGCTAACTCCATTAGGCTTACAACAAGTAAGAGACAGTGGTAAATCAATTCTTTTCTTATTGGAATCAAATCCTGGTCCAGGTTTGGGTCTCCTTGTTGCTTTCTTAATCTTTTTCTTTAAACGTGATAAAAAGCTTTCTAGTAATGCGGCATCTTCTAGTCCTATTCACCTCTTTGGTGGTATCCATGAAGTTTACTTTCCGTTTGTGCTATTAAAACCTGTGTTAATTCTGGCCACAATTGCTGTTGGTGTTGTGGGAAACGGCATTTTGCAAATATTTAATGCTGGGACAATTGCACCAGTGTCACCTGGTTCTGTTATTGCTGGTTTTCTCCAAATTAATAAAACACCTTTAGATGTAGCTGGTTATGCTTTGGCACTCGTTTTATCAGCTGTAACTTCGTTGTTAATTTCGCTTTTATTACTTTCTTTAACACGTAAAAAACAATTGAAAACACTGCAAGAAGCGCAAGCACAAGTTGCAGAAATGAAGCAAACGCCTGCAAAAAAACCAAGACAAAAAGATACTCCAGCAATTGCTACAAAGATAGACTTTTCCCAAGTTACCTTTGTTTGCGATGCAGGTATGGGTTCTTCTACTATGGGAGCAGCTATATTTAGAAAAGAACTAAAAAATCAAAATATTGAAGACATTACAGTGATTAATAAAGCAATAGTTGATTTAAAAGATGAAAAGGTAATTATTACCATTTCCCAACTTTATGATCGCGTAAAAGCGAAGCGTGCAGACGCTACGATTTATACCATCAATCAGTTCTTAGATAAACAAGGCTATTTAACTATTATTGAAAAGATTAAAAATGAAAAGAATTAA", "aa": "MLVNNFYLGFLGFALIFPSFYLSIYLIGYIQLGLKLLVEIMQQYKLYPIAAIVIEPAKVLFLNNAINHGVLTPLGLQQVRDSGKSILFLLESNPGPGLGLLVAFLIFFFKRDKKLSSNAASSSPIHLFGGIHEVYFPFVLLKPVLILATIAVGVVGNGILQIFNAGTIAPVSPGSVIAGFLQINKTPLDVAGYALALVLSAVTSLLISLLLLSLTRKKQLKTLQEAQAQVAEMKQTPAKKPRQKDTPAIATKIDFSQVTFVCDAGMGSSTMGAAIFRKELKNQNIEDITVINKAIVDLKDEKVIITISQLYDRVKAKRADATIYTINQFLDKQGYLTIIEKIKNEKN", "aa_hexdigest": "ccf02f6cefe23cf2bd81e0cd3c7459f0", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001F2174FA", "query_cov": 1.0, "subject_cov": 0.8321342925659473, "identity": 0.9940000000000001, "score": 632.0, "evalue": 4.72e-227, "valid": true, "product": "PTS mannitol transporter subunit IICB", "ec_ids": [ "2.7.1.197" ], "uniref50_id": "UniRef50_UPI001F2174FA", "kegg_orthology_id": "K02800" }, "pscc": { "uniref50_id": "UniRef50_UPI001F2174FA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F2174FA" ], "product": "PTS mannitol transporter subunit IICB" }, "genes": [], "id": "APKECPOBEN_1377", "locus": "GCA002090215_01377" }, { "type": "cds", "sequence": "contig_1", "start": 777504, "stop": 777707, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01232.29" ], "nt": "ATGAAAAGAATTAATGTATTGCACTTTGGCGCTGGTAATATAGGTAGAGGTGTTATATTACCGATTTACCAACAAAATGACTTTTCCATTGATTTAGTGGAATTGAATCAAAATACAGTTAATGAGTTACAAAAGCAAAAACAATACCAAGTTCATTACCTTGATTGTGATCAAAGTCAGTTAGTTAATGACTTTAATACTTGA", "aa": "MKRINVLHFGAGNIGRGVILPIYQQNDFSIDLVELNQNTVNELQKQKQYQVHYLDCDQSQLVNDFNT", "aa_hexdigest": "ddda0e9fd35972435ab7f37929199393", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01232.29", "name": "Mannitol_dh", "length": 62, "aa_cov": 0.9104477611940298, "hmm_cov": 1.0, "evalue": 1.0432625424831932e-21, "score": 73.26538848876953, "start": 5, "stop": 65 } ], "seq_stats": { "molecular_weight": 7779.6486, "isoelectric_point": 5.393133735656738 }, "id": "APKECPOBEN_1378", "locus": "GCA002090215_01378" }, { "type": "cds", "sequence": "contig_1", "start": 777741, "stop": 778511, "strand": "+", "frame": 3, "gene": "mtlD", "product": "mannitol-1-phosphate 5-dehydrogenase", "db_xrefs": [ "COG:COG0246", "COG:G", "EC:1.1.1.17", "GO:0005829", "GO:0008926", "GO:0019592", "KEGG:K00009", "SO:0001217", "UniRef:UniRef50_P78008", "UniRef:UniRef90_P78008" ], "nt": "ATGGAAAGAGCTGATGTTATTTCCACTTCAATAGGTGCTAAAAACTTAGCTAGTTTAAAAACTTTGTTTGACAAAGCTAAGTTCCATAAAAGGGCTATAGTGCTTTGCTTTGAAAACGGCTTTCGGATTAGTTCTAACTTTAAAAACATTTTGCAATTAAATAACACACAAGTAAATTTTGTGGATGTGGTAATCGATACAATTGCACCTAACTTCGAAAAGAAAGCGAATTTCTTGGATATTTATTGCGAGAAGTATTCAGAAATTTATGCTGAAACTTTCCCGCTAGAAATTAAGGGAGTAAATCAAAAGAATAGTTTAGATAGATTCATTATTAAAAAACTTTTATTAGTCAATGCACTGCACAGTGTTATTGGACTTTTAGGCTTTCAACAAAAGCTTAAGTATGTACATGAAACATTGCAAGTTAAAAGTAATTTGACTTTTGTAGAAAAATTAGCCCAACAAATTATTGATGCCCTTTGTGCTGAATATCCTGAATTTAATAAAAATAATTTGTTAAGTTATGGCAAAAATAACTTAGTCCGTTTTGCTAATCCCAAGATTCAAGATTTAAACACACGCTTAATTAGGGAACCATTACGTAAACTTAACCAAAATGAACGCTTTTATGCCATTTATAAACTCTTTAAAAAGAATAAGATTGCACTAAATAATATTTTGCAAGTTTATCTGATGGTTTTAAAAACTAATATAACAGATGATACCGAAAGCCAACAAATTGCTAAATTAATAAATGAAAAAGCTTGA", "aa": "MERADVISTSIGAKNLASLKTLFDKAKFHKRAIVLCFENGFRISSNFKNILQLNNTQVNFVDVVIDTIAPNFEKKANFLDIYCEKYSEIYAETFPLEIKGVNQKNSLDRFIIKKLLLVNALHSVIGLLGFQQKLKYVHETLQVKSNLTFVEKLAQQIIDALCAEYPEFNKNNLLSYGKNNLVRFANPKIQDLNTRLIREPLRKLNQNERFYAIYKLFKKNKIALNNILQVYLMVLKTNITDDTESQQIAKLINEKA", "aa_hexdigest": "2cd67a8114f1c81fcfb5bc6c24245e77", "start_type": "ATG", "rbs_motif": null, "genes": [ "mtlD" ], "truncated": "both", "pseudogene": { "start": 777504, "stop": 778598, "observations": { "mutation": [ 777705, 778509, 778551 ], "stop": [ 777705, 778509, 778551 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P78008", "query_cov": 1.0, "subject_cov": 0.7032967032967034, "identity": 1.0, "score": 495.0, "evalue": 4.07e-175, "gene_start": 1, "gene_end": 256, "reference_start": 80, "reference_end": 335, "reference_sequence": "MKRINVLHFGAGNIGRGVILPIYQQNDFSIDLVELNQNTVNELQKQKQYQVHYLDCDQSQLVNDFNTWNLKDEAKIIELMERADVISTSIGAKNLASLKTLFDKAKFHKRAIVLCFENGFRISSNFKNILQLNNTQVNFVDVVIDTIAPNFEKKANFLDIYCEKYSEIYAETFPLEIKGVNQKNSLDRFIIKKLLLVNALHSVIGLLGFQQKLKYVHETLQVKSNLTFVEKLAQQIIDALCAEYPEFNKNNLLSYGKNNLVRFANPKIQDLNTRLIREPLRKLNQNERFYAIYKLFKKNKIALNNILQVYLMVLKTNITDDTESQQIAKLINEKAWTELAKLSSLEESEWNLIKQELSREITKK" }, "paralog": false, "identity": 0.9917582417582418, "score": 699.0, "evalue": 4.01e-258, "description": "Internal stop codon at 777705, 778509, 778551. Nonsense mutation around 777705, 778509, 778551.", "psc": { "uniref90_id": "UniRef90_P78008", "gene": "mtlD", "product": "mannitol-1-phosphate 5-dehydrogenase", "ec_ids": [ "1.1.1.17" ], "uniref50_id": "UniRef50_P78008", "cog_id": "COG0246", "cog_category": "G", "kegg_orthology_id": "K00009", "go_ids": [ "GO:0005829", "GO:0008926", "GO:0019592" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P78008", "EC:1.1.1.17", "UniRef:UniRef50_P78008", "COG:COG0246", "COG:G", "KEGG:K00009", "GO:0005829", "GO:0008926", "GO:0019592" ] }, "pscc": { "uniref50_id": "UniRef50_P78008", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P78008" ], "product": "Mannitol-1-phosphate 5-dehydrogenase" } }, "id": "APKECPOBEN_1379", "locus": "GCA002090215_01379" }, { "type": "cds", "sequence": "contig_1", "start": 778579, "stop": 779010, "strand": "+", "frame": 1, "gene": "mtlF", "product": "Mannitol-specific phosphotransferase enzyme IIA component", "db_xrefs": [ "COG:COG4668", "COG:G", "GO:0005737", "GO:0005886", "GO:0009401", "GO:0016301", "GO:0090563", "RefSeq:WP_010875010.1", "SO:0001217", "UniParc:UPI000013295B", "UniRef:UniRef100_P75145", "UniRef:UniRef50_P75145", "UniRef:UniRef90_P75145" ], "nt": "GTGAAATTACTAAAAAATAACATTTACATTAACGTTTATTTAAAAAACAAACAAGAAATTTTTGAATTTGTTTTTAAAAAGTTTAAAGAAGATGGTGCTGTACTAGATAGTTTTTTACCAGCAATTGTGGAACGCGATAAAGCAGCTAGTGTAGCAATTGGTAATTACCTCTTTTTACCCCACCCAGTTTATGACGAAATAGCAAACATTCAAAAAGAAAAAATGGTGTTTATTGGTTTAAAAGATGTAATTAACATTGATGGTCAACCAATTAAATTTATCTGTGGTTTAGCCTTAAAAGGTGAGCACCAAATGGATGCGTTGCAATCTTTAGCAATAGCTTTTTCAGACCCTGAAGAAGTGGAAAAGTTAGTTAAAGATAAGGATTTAACACAAGATAAGGTACTTGAATTTTTAGCAAAACACAACTAA", "aa": "MKLLKNNIYINVYLKNKQEIFEFVFKKFKEDGAVLDSFLPAIVERDKAASVAIGNYLFLPHPVYDEIANIQKEKMVFIGLKDVINIDGQPIKFICGLALKGEHQMDALQSLAIAFSDPEEVEKLVKDKDLTQDKVLEFLAKHN", "aa_hexdigest": "c06514f8b7e06b0a390b0475ecc1436f", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013295B", "ncbi_nrp_id": "WP_010875010.1", "uniref100_id": "UniRef100_P75145", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013295B", "RefSeq:WP_010875010.1", "UniRef:UniRef100_P75145" ] }, "ips": { "uniref100_id": "UniRef100_P75145", "uniref90_id": "UniRef90_P75145" }, "psc": { "uniref90_id": "UniRef90_P75145", "gene": "mtlF", "product": "Mannitol-specific phosphotransferase enzyme IIA component", "uniref50_id": "UniRef50_P75145", "cog_id": "COG4668", "cog_category": "G", "go_ids": [ "GO:0005737", "GO:0005886", "GO:0016301", "GO:0090563", "GO:0009401" ] }, "pscc": { "uniref50_id": "UniRef50_P75145", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75145" ], "product": "Mannitol-specific phosphotransferase enzyme IIA component" }, "genes": [ "mtlF" ], "id": "APKECPOBEN_1380", "locus": "GCA002090215_01380" }, { "type": "cds", "sequence": "contig_1", "start": 779518, "stop": 779772, "strand": "-", "frame": 1, "gene": null, "product": "putative lipoprotein MPN_467", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75316" ], "nt": "ATGTTAATGCGTAAAAAGAAACTTTTGTCAAGGATTTCCTTCGGTTCTTTGTTTTTGTTATGTGGAACAATATTAAGTGCTTGCACATGGATCCAAGCCAATCTTAGGAATCTTCTTAAAGAAACAACTGGCAAAGATTTTGATTTGTCTAAAGCAATTAAAATTCCGGAAGGTAGGCAAAACCTCATAAATTCTTTAAAGAAATCTTATGAAGTTAATCCAAAAGATACAACAAAGCTATTGTTAGACGCATGA", "aa": "MLMRKKKLLSRISFGSLFLLCGTILSACTWIQANLRNLLKETTGKDFDLSKAIKIPEGRQNLINSLKKSYEVNPKDTTKLLLDA", "aa_hexdigest": "1b5af5852a8f105a0151a9168d3fd15f", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75316", "query_cov": 0.9761904761904762, "subject_cov": 0.8118811881188119, "identity": 0.8290000000000001, "score": 133.0, "evalue": 5.08e-39, "valid": false, "product": "Uncharacterized lipoprotein MPN_467", "uniref50_id": "UniRef50_P75316", "go_ids": [ "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75316", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75316" ], "product": "Uncharacterized lipoprotein MPN_467" }, "genes": [], "id": "APKECPOBEN_1381", "locus": "GCA002090215_01381" }, { "type": "cds", "sequence": "contig_1", "start": 780049, "stop": 780663, "strand": "+", "frame": 1, "gene": null, "product": "UPF0134 protein MPN_655", "db_xrefs": [ "RefSeq:WP_010875012.1", "SO:0001217", "UniParc:UPI000013AFBA", "UniRef:UniRef100_P75136", "UniRef:UniRef50_P75136", "UniRef:UniRef90_P75136" ], "nt": "ATGAGCAACAATAATAAATATTTCACTATAACTAAAAAACAGTACAAAAAAATGCGCCGCAATAAAATTGAACTTCTTTTTAATGTGAAAGTCCTAAAAAAGAAAAACGGTCGACAAAAGTTCAAAATACTGCATGAAGTTGAGAATAAGCCAAAAATACCAATTAAAATAATTGAAGATCAACCGGAGTCGCCAAAGCCACTTAAACCCCCTAAACCGCCTAAACCGCCTAAAGGTCCTGACAATCCTGAGGAGCCGGATTCTCCAGAAGAACCCAAAGAAACAGATCAACCAGGTGGTCCCGATAATCCTAATGCAGGAAATAAAAAGATGCCAACACCTGAAGAATACGTAACTCGTAAAGAATTCAACGAATTCAAAGATTCAAACAATCAACGACTTACAAAGATTGAAAATAAGGTTGATAAGTTAGAAGTCAAAGTCGACAAATTAGCTGAAATTGTTCAAACTCAGGGCGAAGAAATCAAAGAACTGAAAGTGGAGCAAAAAGCCCAGAGTGAAACCCTACAGCTAATTTTGAAAACACTGCAAAAGATGAACGATCGTCTGGACAGAATGGAAACTCGCTTAGACAAGATCGATTCGCCCAAATAG", "aa": "MSNNNKYFTITKKQYKKMRRNKIELLFNVKVLKKKNGRQKFKILHEVENKPKIPIKIIEDQPESPKPLKPPKPPKPPKGPDNPEEPDSPEEPKETDQPGGPDNPNAGNKKMPTPEEYVTRKEFNEFKDSNNQRLTKIENKVDKLEVKVDKLAEIVQTQGEEIKELKVEQKAQSETLQLILKTLQKMNDRLDRMETRLDKIDSPK", "aa_hexdigest": "779fa67d3db9eb70dec7aadf62938d21", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013AFBA", "ncbi_nrp_id": "WP_010875012.1", "uniref100_id": "UniRef100_P75136", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013AFBA", "RefSeq:WP_010875012.1", "UniRef:UniRef100_P75136" ] }, "ips": { "uniref100_id": "UniRef100_P75136", "uniref90_id": "UniRef90_P75136" }, "psc": { "uniref90_id": "UniRef90_P75136", "product": "UPF0134 protein MPN_655", "uniref50_id": "UniRef50_P75136" }, "pscc": { "uniref50_id": "UniRef50_P75136", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75136" ], "product": "UPF0134 protein MPN_655" }, "genes": [], "id": "APKECPOBEN_1382", "locus": "GCA002090215_01382" }, { "type": "cds", "sequence": "contig_1", "start": 780848, "stop": 781027, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W5N8", "UniRef:UniRef90_A0AB38W5N8" ], "nt": "ATGAAATATAAAAAGATAGGTGATATTAAATCTAAAGGATTTTTCGAATCTAAAGAAGAATATAAGGAACTTTTAAAAAAATGTGAACTAATTTCTTTTGAAGCTTTTTCTGAAAAATTACCTAATGGTGATTATGAAGTAACTAAGAAACTTAAGCTTGTAGAAAAAGGTCCAAATTGA", "aa": "MKYKKIGDIKSKGFFESKEEYKELLKKCELISFEAFSEKLPNGDYEVTKKLKLVEKGPN", "aa_hexdigest": "a4126a45b83c3d27106973a8370f4762", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 780777, "stop": 781096, "observations": { "deletion": [ 780849 ], "stop": [ 781026 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB38W5N8", "query_cov": 1.0, "subject_cov": 0.5566037735849056, "identity": 1.0, "score": 117.0, "evalue": 7.41e-33, "gene_start": 1, "gene_end": 59, "reference_start": 25, "reference_end": 83, "reference_sequence": "NLFSGWAKCNFFDFLESSRKEVLIMKYKKIGDIKSKGFFESKEEYKELLKKCELISFEAFSEKLPNGDYEVTKKLKLVEKGPNWKRKTYKQSLENEKILKWLIEHD" }, "paralog": false, "identity": 0.9811320754716981, "score": 204.0, "evalue": 1.07e-69, "description": "Internal stop codon at 781026. Frameshift due to deletion around 780849.", "psc": { "uniref90_id": "UniRef90_A0AB38W5N8", "uniref50_id": "UniRef50_A0AB38W5N8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB38W5N8", "UniRef:UniRef50_A0AB38W5N8" ] }, "pscc": { "uniref50_id": "UniRef50_A0AB38W5N8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W5N8" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 6904.994200000001, "isoelectric_point": 8.990712547302245 }, "id": "APKECPOBEN_1383", "locus": "GCA002090215_01383" }, { "type": "cds", "sequence": "contig_1", "start": 781136, "stop": 781456, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAGCGATACCCCAGGAGTCTTCTTTAAACGAATTGATGAAATGGCTGTTGGTTACAAGTTAGTTTTAACTAACGTTATTAAAAGGGAAGTAGTCCCTTTGGAAGATGTAGGTGCGTTTGCTTTTTGTTATTTAAGCAAACATTATCCGCAATTGTTACCATATGAAGGTACAGACTTCACTGAGTTTTTACATAAATTTGCTATCAGTCGTAAGTTGTTGCAAAAATCGAATCAGCTCAACATTAATTTAGCGTGCGAACTGTTTGTTAGTGAACTGATAAACGGGAAGTACGGTAAACTGAGTTTTGAGTTAGATTAG", "aa": "MSDTPGVFFKRIDEMAVGYKLVLTNVIKREVVPLEDVGAFAFCYLSKHYPQLLPYEGTDFTEFLHKFAISRKLLQKSNQLNINLACELFVSELINGKYGKLSFELD", "aa_hexdigest": "9ca259566b1ccafaf0ec49739a05e34a", "start_type": "TTG", "rbs_motif": "AATAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12155.996799999988, "isoelectric_point": 6.096573066711424 }, "id": "APKECPOBEN_1384", "locus": "GCA002090215_01384" }, { "type": "cds", "sequence": "contig_1", "start": 781481, "stop": 781951, "strand": "-", "frame": 3, "gene": "ylqF", "product": "ribosome biogenesis GTPase YlqF", "db_xrefs": [ "COG:COG1161", "COG:J", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0006412", "GO:0042254", "SO:0001217", "UniRef:UniRef50_P75135", "UniRef:UniRef90_P75135" ], "nt": "ATGGATACATTTACCTCCGCAGTCAAGATTAACTGGTTTCCGGGTCACATGAAGAAGACCCATGACCAACTCAAAAAACTAGCTAGTAGTTTAGATGGTGTAATTGAGGTAGTTGATGCGCGTGCACCAACGTTAACCCAAAACCCCGAAATTACGGCTTACTTTACCAATAAACCGAAACTCACTTTAGCTTTAAAGGCTGATTTAGCACAAACAGTGGCTAACTCCAATATTTTATGGGGAACCTTAAAGCAAGGTTTGCAACTTAAACGCTTAGTTATTAAGAAACTGCAAACTTTGTTTCAAGCAAAAAAGAATCAGTTAAAAGCCAAAGGTTTATTAGTGCACCAGTTTCGGTTAGCGGTTATTGGAATGCCCAACGTTGGTAAATCCAGTTTAATTAACTTGTTACTAAATAAAAATCACTTACAGGTAGCTAATCGGGCTGGGGTTACCAAGTCAATGAGTTGA", "aa": "MDTFTSAVKINWFPGHMKKTHDQLKKLASSLDGVIEVVDARAPTLTQNPEITAYFTNKPKLTLALKADLAQTVANSNILWGTLKQGLQLKRLVIKKLQTLFQAKKNQLKAKGLLVHQFRLAVIGMPNVGKSSLINLLLNKNHLQVANRAGVTKSMS", "aa_hexdigest": "26aecbf46c5a5e62f936b20eae5dd902", "start_type": "ATG", "rbs_motif": null, "genes": [ "ylqF" ], "truncated": "5-prime", "pseudogene": { "start": 781178, "stop": 781951, "observations": { "mutation": [ 781483 ], "stop": [ 781483 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75135", "query_cov": 1.0, "subject_cov": 0.5756457564575646, "identity": 1.0, "score": 300.0, "evalue": 4.05e-101, "gene_start": 1, "gene_end": 156, "reference_start": 1, "reference_end": 156, "reference_sequence": "MDTFTSAVKINWFPGHMKKTHDQLKKLASSLDGVIEVVDARAPTLTQNPEITAYFTNKPKLTLALKADLAQTVANSNILWGTLKQGLQLKRLVIKKLQTLFQAKKNQLKAKGLLVHQFRLAVIGMPNVGKSSLINLLLNKNHLQVANRAGVTKSMSWNQISSEFYLSDTPGVFFKRIDEMAVGYKLVLTNVIKREVVPLEDVGAFAFCYLSKHYPQLLPYEGTDFTEFLHKFAISRKLLQKSNQLNINLACELFVSELINGKYGKLSFELD" }, "paralog": false, "identity": 0.9961089494163424, "score": 502.0, "evalue": 2.43e-183, "description": "Internal stop codon at 781483. Nonsense mutation around 781483.", "psc": { "uniref90_id": "UniRef90_P75135", "gene": "ylqF", "product": "ribosome biogenesis GTPase YlqF", "uniref50_id": "UniRef50_P75135", "cog_id": "COG1161", "cog_category": "J", "go_ids": [ "GO:0003924", "GO:0005525", "GO:0005737", "GO:0006412", "GO:0042254" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75135", "UniRef:UniRef50_P75135", "COG:COG1161", "COG:J", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0006412", "GO:0042254" ] }, "pscc": { "uniref50_id": "UniRef50_P75135", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75135" ], "product": "Probable ribosome biogenesis GTPase A" } }, "id": "APKECPOBEN_1385", "locus": "GCA002090215_01385" }, { "type": "cds", "sequence": "contig_1", "start": 781920, "stop": 782231, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF10035.14" ], "nt": "GTGTTATTTACCGGTACAGTAGTGAGTCACCTTTTCCCAAGGTATAACTTTGCTGAAATTAAGGTCTTTACTGACAAGATTGAAGAAGTGAGGTTGGCTCTGCTCAACGATAAAGCGACTCACAGTCTGTCCATTCAAGAAACGATGGGTGGATACTCATTAGCCAAAAAACGGATGATAGTTTCAGTAACTATGTATGTGGAAATTCCTAACCTCATCCGCATCATCCGGAAAATTGACAAAGATTGTTTAGTATCAATTACAAGGATCCGAGGGATCGATGGATACATTTACCTCCGCAGTCAAGATTAA", "aa": "MLFTGTVVSHLFPRYNFAEIKVFTDKIEEVRLALLNDKATHSLSIQETMGGYSLAKKRMIVSVTMYVEIPNLIRIIRKIDKDCLVSITRIRGIDGYIYLRSQD", "aa_hexdigest": "33dee91c770c25c59834e4626163ef0f", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF10035.14", "name": "DUF2179", "length": 52, "aa_cov": 0.5048543689320388, "hmm_cov": 1.0, "evalue": 2.4635339041687447e-11, "score": 38.782386779785156, "start": 44, "stop": 95 } ], "seq_stats": { "molecular_weight": 11882.873999999985, "isoelectric_point": 9.371398353576659 }, "id": "APKECPOBEN_1386", "locus": "GCA002090215_01386" }, { "type": "cds", "sequence": "contig_1", "start": 782280, "stop": 782465, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGGATCTTGTGCTGGGGGTGCCGATTTCCTAACGCAATACTTTGCGAGAAAGAAAAACCGCTCCGTGGGGCCAATTCTCTTTTATGTCAATACCTTTATTTTGATCATTGCGATCTTAATGGGTTCGTTTGTTGCTGGCAGTATTGTGCTCCAAGATATTCCCGATTACAAAAAAAGTGCTTGA", "aa": "MGSCAGGADFLTQYFARKKNRSVGPILFYVNTFILIIAILMGSFVAGSIVLQDIPDYKKSA", "aa_hexdigest": "0644a8e2ac08d27f410a46c58827d58e", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6618.7403, "isoelectric_point": 9.35966510772705 }, "id": "APKECPOBEN_1387", "locus": "GCA002090215_01387" }, { "type": "cds", "sequence": "contig_1", "start": 782562, "stop": 783029, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTAGTGCCCTTTATGAGGCTAAAAAACCTTTAAAGTACACAATTGTCTACATCCTGGCTTTAGTGAACGCCTTCTTTTTGCTGGTGTTTATCCAACAAACGGGATTGTACAGCTTTGGGATTAGTTCGCTAACCCAAGGTTTTGCGCGTTTACTGTTTGTCTTGTTGAAGAACCTCGAAGATGGACAACGCAACCTAGTGTTCAACATCTTTTACTGGCTGTTTTATGTAATAGTCAACATTCCCCTGATTATTTTTTCTTACAAAAAAATTGGGAAACGTTTCACCATTCTGTCAACCCATTACGTGGTCGCTTCCAACGTCTTTTGTTTTATCTTTAGTATTATTCCGGGGGCTAATCAATTGCCATCGATGTTGTCCGCAGTGCACCACACTGAATTTTGGGAAGCTGCAAAAAAAGCTGAAGGTGTTGATCAAAGCGCGTTATTTGTACCGTTCTTATGA", "aa": "MFSALYEAKKPLKYTIVYILALVNAFFLLVFIQQTGLYSFGISSLTQGFARLLFVLLKNLEDGQRNLVFNIFYWLFYVIVNIPLIIFSYKKIGKRFTILSTHYVVASNVFCFIFSIIPGANQLPSMLSAVHHTEFWEAAKKAEGVDQSALFVPFL", "aa_hexdigest": "c1084168e775933201ea69c6cdf83371", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17738.810499999985, "isoelectric_point": 9.380488395690918 }, "id": "APKECPOBEN_1388", "locus": "GCA002090215_01388" }, { "type": "cds", "sequence": "contig_1", "start": 783141, "stop": 783500, "strand": "-", "frame": 2, "gene": "rplS", "product": "50S ribosomal protein L19", "db_xrefs": [ "COG:COG0335", "COG:J", "GO:0000311", "GO:0003735", "GO:0006412", "GO:0022625", "KEGG:K02884", "RefSeq:WP_010875015.1", "SO:0001217", "UniParc:UPI0000133CB4", "UniRef:UniRef100_P75133", "UniRef:UniRef50_Q8EWU9", "UniRef:UniRef90_P75133" ], "nt": "ATGAAAAAAATAAATAAACAAGCTTTAATAGATTTAGTAGAACAAAAGCAATTAAAGGCTTATGTGCCTGAGTTTAGCGCTGGTGACGAAGTGAATGTTGCCATCAAACTTAAAGAAAAGGAAAAGGTCAGAATCCAAAACTTTACGGGAACTGTCCTACGCCGTCGTGGTAAAGGTATTAGTGAAACCTTCATTGTGCGTAAAACCACTGATGGTATTCCGATTGAAAAAAACTTCCAAATTCATAACCCTAATATTTCAATTGAATTGAAAAGACGGGGTAAAGTAAGACGCGCTTACATTTCATATATGCGTGAGCGCTCTGGTAAAGCTGCCAAAATTAAAGAGCGAAAACAATAG", "aa": "MKKINKQALIDLVEQKQLKAYVPEFSAGDEVNVAIKLKEKEKVRIQNFTGTVLRRRGKGISETFIVRKTTDGIPIEKNFQIHNPNISIELKRRGKVRRAYISYMRERSGKAAKIKERKQ", "aa_hexdigest": "5d27fe9483784a60add336e9224bdb23", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000133CB4", "ncbi_nrp_id": "WP_010875015.1", "uniref100_id": "UniRef100_P75133", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000133CB4", "RefSeq:WP_010875015.1", "UniRef:UniRef100_P75133" ] }, "ips": { "uniref100_id": "UniRef100_P75133", "uniref90_id": "UniRef90_P75133" }, "psc": { "uniref90_id": "UniRef90_P75133", "gene": "rplS", "product": "50S ribosomal protein L19", "uniref50_id": "UniRef50_Q8EWU9", "cog_id": "COG0335", "cog_category": "J", "kegg_orthology_id": "K02884", "go_ids": [ "GO:0000311", "GO:0003735", "GO:0006412", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_Q8EWU9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q8EWU9" ], "product": "Large ribosomal subunit protein bL19" }, "genes": [ "rplS" ], "id": "APKECPOBEN_1389", "locus": "GCA002090215_01389" }, { "type": "cds", "sequence": "contig_1", "start": 783484, "stop": 784026, "strand": "-", "frame": 1, "gene": null, "product": "tRNA (guanine-N(1)-)-methyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B1GZ20" ], "nt": "ATGGCTTACGGAGGAGGTGGCGGGATGGTTCTAAAAGCTGAACCAATTCTCAAAGCTTTAAAGGCTTGTAGAACTCCACAATCGAAAGTAGTTTTATTATCACCTGAGGGACAACAGTTTTCCCAACCCATGGCGCAAGCTTTAACCCAAACGGAACACCTCATTTTGATCTGTGGTCACTATGAGGGCTTTGATTACCGCTTGTATAAACATGTCGATCAAATTATTTCGTTAGGTGACTTTGTGTTGAGTGGTGGGGAGTTAGTGGCTTTAAGTGTGATTGATGCCACTGTTCGTTTAATTAAGGGTGTCATTAATGATCAGAGCTTAATCCACGAATCATTTAACAACTACTTACTGGATTTCCCCGCTTACACTAGGCCATATGATCTAGACGGGGATAAGGTACCTGAAATATTATTATCAGGTGACCATAAAAAGATAGAGGCTTATCGCAAAGAACAACAACTGCTCAGAACGGCTCAATACCGTCCTGATTTGTATAAGCAATATTTAGCAAAGAAAGATGAAAAAAATAAATAA", "aa": "MAYGGGGGMVLKAEPILKALKACRTPQSKVVLLSPEGQQFSQPMAQALTQTEHLILICGHYEGFDYRLYKHVDQIISLGDFVLSGGELVALSVIDATVRLIKGVINDQSLIHESFNNYLLDFPAYTRPYDLDGDKVPEILLSGDHKKIEAYRKEQQLLRTAQYRPDLYKQYLAKKDEKNK", "aa_hexdigest": "51ad4a2c39a9875da92070ad9cf04f7b", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A534NV99", "query_cov": 0.9444444444444444, "subject_cov": 0.821256038647343, "identity": 0.541, "score": 171.0, "evalue": 4.88e-51, "valid": false, "gene": "trmD", "product": "tRNA (guanosine(37)-N1)-methyltransferase TrmD", "ec_ids": [ "2.1.1.228" ], "uniref50_id": "UniRef50_B1GZ20", "kegg_orthology_id": "K00554", "go_ids": [ "GO:0008033", "GO:0052906" ] }, "pscc": { "uniref50_id": "UniRef50_B1GZ20", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B1GZ20" ], "product": "tRNA (guanine-N(1)-)-methyltransferase" }, "genes": [], "id": "APKECPOBEN_1390", "locus": "GCA002090215_01390" }, { "type": "cds", "sequence": "contig_1", "start": 784293, "stop": 784442, "strand": "-", "frame": 2, "gene": "rpsP", "product": "30S ribosomal protein S16", "db_xrefs": [ "COG:COG0228", "COG:J", "GO:0000314", "GO:0003735", "GO:0005737", "GO:0006412", "GO:0015935", "GO:0022627", "SO:0001217", "UniRef:UniRef50_P47684", "UniRef:UniRef90_P75131" ], "nt": "ATGGGTCGTGTTCACTACCCAACCTACAGAATAGTAGCTGTTGATTCCAGAGTTAAACGTGATGGTAAGTACATTGCGTTAATTGGCCATCTCAATCCTGCATTAAAAGAAAATAAGTGCAAGATTGATGAAGCTGTGGCCTTGGAATGA", "aa": "MGRVHYPTYRIVAVDSRVKRDGKYIALIGHLNPALKENKCKIDEAVALE", "aa_hexdigest": "637d190f7323f0450db83dfbabd2a9be", "start_type": "ATG", "rbs_motif": "AAAA", "genes": [ "rpsP" ], "truncated": "5-prime", "pseudogene": { "start": 784182, "stop": 784466, "observations": { "mutation": [ 784295 ], "stop": [ 784295 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75131", "query_cov": 1.0, "subject_cov": 0.5212765957446809, "identity": 1.0, "score": 102.0, "evalue": 1.49e-27, "gene_start": 1, "gene_end": 49, "reference_start": 9, "reference_end": 57, "reference_sequence": "MVKIRLMRMGRVHYPTYRIVAVDSRVKRDGKYIALIGHLNPALKENKCKIDEAVALEWLNKGAKPTDTVRSLFSQTGLWKKFVESKKKPVAKSK" }, "paralog": false, "identity": 0.9787234042553191, "score": 184.0, "evalue": 2.84e-62, "description": "Internal stop codon at 784295. Nonsense mutation around 784295.", "psc": { "uniref90_id": "UniRef90_P75131", "gene": "rpsP", "product": "30S ribosomal protein S16", "uniref50_id": "UniRef50_P47684", "cog_id": "COG0228", "cog_category": "J", "go_ids": [ "GO:0000314", "GO:0003735", "GO:0005737", "GO:0006412", "GO:0015935", "GO:0022627" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75131", "UniRef:UniRef50_P47684", "COG:COG0228", "COG:J", "GO:0000314", "GO:0003735", "GO:0005737", "GO:0006412", "GO:0015935", "GO:0022627" ] }, "pscc": { "uniref50_id": "UniRef50_P47684", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47684" ], "product": "Small ribosomal subunit protein bS16" } }, "id": "APKECPOBEN_1391", "locus": "GCA002090215_01391" }, { "type": "cds", "sequence": "contig_1", "start": 784530, "stop": 784754, "strand": "-", "frame": 2, "gene": "norM", "product": "putative MG447-like protein", "db_xrefs": [ "COG:COG0534", "COG:V", "GO:0005886", "GO:0015297", "GO:0042910", "RefSeq:WP_243679576.1", "SO:0001217", "UniParc:UPI000AC194D4", "UniRef:UniRef100_P75130", "UniRef:UniRef50_P47685", "UniRef:UniRef90_P75130" ], "nt": "ATGCAAGGGGTTATTGTTTTTGTACCCTTGTTGTTTATCTTTCAAGCAATTACCTTACAAACAAAAAACATTGAAATCTTTATCTGGTTATTAACGGCTAACGCTGCTCTGGCTGGATTGATTAACGTTTTGATTGGCCAAGTACACATCCACTTCTTTATGGATAAATACTTCGCTCAAAAACATAAGAGTCGGATTGTCCAGTTCATTGAGCGCTATTCTTAG", "aa": "MQGVIVFVPLLFIFQAITLQTKNIEIFIWLLTANAALAGLINVLIGQVHIHFFMDKYFAQKHKSRIVQFIERYS", "aa_hexdigest": "3b5ab56965889da8b0b63069a76e0208", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000AC194D4", "ncbi_nrp_id": "WP_243679576.1", "uniref100_id": "UniRef100_P75130", "db_xrefs": [ "SO:0001217", "UniParc:UPI000AC194D4", "RefSeq:WP_243679576.1", "UniRef:UniRef100_P75130" ] }, "ips": { "uniref100_id": "UniRef100_P75130", "uniref90_id": "UniRef90_P75130" }, "psc": { "uniref90_id": "UniRef90_P75130", "gene": "NorM", "product": "Uncharacterized protein MG447 homolog", "uniref50_id": "UniRef50_P47685", "cog_id": "COG0534", "cog_category": "V", "go_ids": [ "GO:0005886", "GO:0015297", "GO:0042910" ] }, "pscc": { "uniref50_id": "UniRef50_P47685", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47685" ], "product": "Uncharacterized protein MG447" }, "genes": [ "norM" ], "id": "APKECPOBEN_1392", "locus": "GCA002090215_01392" }, { "type": "cds", "sequence": "contig_1", "start": 784767, "stop": 786179, "strand": "-", "frame": 2, "gene": "norM", "product": "putative MG447-like protein", "db_xrefs": [ "COG:COG0534", "COG:V", "GO:0005886", "GO:0015297", "GO:0042910", "SO:0001217", "UniRef:UniRef50_P47685", "UniRef:UniRef90_P75130" ], "nt": "GTGAATAGCCAACAAGATCGTCTAGCGAAGCAAGTCCTCATCCAAAAATCCTTTGAATCGAAAAGACTGTTTCTAACGATCTTGCGCTTTGCTATTCCGACTTTTTTCTTTGCGCTGTTTTCCGCTGCTTATGTCTTTGTGGACCAAATTATGGTGATTAAGTTTGTCCCGCATGGACCACTTAATCCCGATTCAATCTTTACTGACCAGGCCTTAATTGAGGAATTTAAAGCATCCGCGTTTTATAAGGGTGGGGACATTCCCAACCACACGGAACTAACAGCTAGTCAATTAGTCAAAACAGTCTTAAATATTAGCCAGCCAATTGTAGTCATTCTCAACGCCATTACCATCTTTGTGCCGTTGGGTACGGGGGTGATCTTTTCCAAAACCATTGGTAAGGGTGATGAGAAAAAGATTAAGGATGCTTGGAATACCGGGTTAGTTTCCACTACCTTATTTGCCCTAGTGACCCAAATTATTGTGCTAGCGATTGCCAAAGAGTGGTTGCAGTTTAACTTGGATAAAGTCGATGAACAACACCACGTACAAGTAGCGGATCAGTTTCAACACTTCTTTAACGAAAAGGCGGTTGCGATTGGTTCAGAGTACGTTTACATTTTGATTGGCTTTAACATCATACCGATGTTGAGCCGCTTGTTTTTTTACTTAGGCCAATCAGAGGGCCGGCAACTGTTTATTGCAATTGTGCCTCCTTTATCGAACTTACTGAACGTCCTGTTTGTTTTCTTGTTAGTGCGTTTTTCCACTTTAGGGGTTGTGGGCTCCGCGGTGGCTGCGATTTTGGTTTACTTCATTACCTTTATGGCTTATGTGGTGTACCTTATTAGTTTAAATAAACGTGGTTTAACCTACCTTTCGTTGAGGGACTTTTCCTTTAAACGGGTTAGTTTTAACCTCTTTTTGGTCATTTCAATGGTGGGGTTAGCCTCCTTTTTCCGTAATGGTTCGCTCTCAATTCTCAACACCTTTTACGAAAGCTTTTTGGTGAATTTGACCAAAACGCTTACGGACCAAAGTGATACCTTTTACTTGGTGTTACTAACGGGACCCATTGCAATTGCTAACCTAACTTCAGCCGCCATCTTTGGTGTTCTCCAAGGGGTGCGGACAGTGGTATCCTACAAGTTTGGTCAGGGCCAGTTGGCTGACATTAAAAGGATTAATGTTTATACGTTGTTAGTGTGTTTGGTATTTGCTGCTTTGCTGTATTTAATTCTGGCAGTTGGATTGGGCAAAGAAATCCTAGTGCACCTGTTTGATACAAGTGCAGCTACCTTAATGCTCGCTAATCAGTTTTCCTTAATTGTGCAAGCGCAAGTGTTCTTTGTGGCCATTGGCGCTACCAGTCAACAGTACTTCCAAAATAACAACCGGGTGTTGTATTCGTGA", "aa": "MNSQQDRLAKQVLIQKSFESKRLFLTILRFAIPTFFFALFSAAYVFVDQIMVIKFVPHGPLNPDSIFTDQALIEEFKASAFYKGGDIPNHTELTASQLVKTVLNISQPIVVILNAITIFVPLGTGVIFSKTIGKGDEKKIKDAWNTGLVSTTLFALVTQIIVLAIAKEWLQFNLDKVDEQHHVQVADQFQHFFNEKAVAIGSEYVYILIGFNIIPMLSRLFFYLGQSEGRQLFIAIVPPLSNLLNVLFVFLLVRFSTLGVVGSAVAAILVYFITFMAYVVYLISLNKRGLTYLSLRDFSFKRVSFNLFLVISMVGLASFFRNGSLSILNTFYESFLVNLTKTLTDQSDTFYLVLLTGPIAIANLTSAAIFGVLQGVRTVVSYKFGQGQLADIKRINVYTLLVCLVFAALLYLILAVGLGKEILVHLFDTSAATLMLANQFSLIVQAQVFFVAIGATSQQYFQNNNRVLYS", "aa_hexdigest": "2318a3f2de74384e0f803c80edbd7952", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75130", "query_cov": 1.0, "subject_cov": 0.8561020036429873, "identity": 0.998, "score": 825.0, "evalue": 8.77e-299, "valid": true, "gene": "NorM", "product": "Uncharacterized protein MG447 homolog", "uniref50_id": "UniRef50_P47685", "cog_id": "COG0534", "cog_category": "V", "go_ids": [ "GO:0005886", "GO:0015297", "GO:0042910" ] }, "pscc": { "uniref50_id": "UniRef50_P47685", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47685" ], "product": "Uncharacterized protein MG447" }, "genes": [ "norM" ], "id": "APKECPOBEN_1393", "locus": "GCA002090215_01393" }, { "type": "cds", "sequence": "contig_1", "start": 786180, "stop": 786368, "strand": "-", "frame": 2, "gene": null, "product": "peptide-methionine (R)-S-oxide reductase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0024947FD0" ], "nt": "ATGATCCGCACCGAAGTACGCGCCAAAAACTCCAACAGCCACCTAGGGCACGTCTTTCGTGATGGTCCCGAAGAGCATGGTGGTTTGCGTTACTGCATTAACTCCGCTGCTCTAAAGTTCATTCCGTTTGCGGAAATGGAATCGGCGGGGTATGGTGAATACCTCAAATTATTTAAGGAATCTAACTAA", "aa": "MIRTEVRAKNSNSHLGHVFRDGPEEHGGLRYCINSAALKFIPFAEMESAGYGEYLKLFKESN", "aa_hexdigest": "280923d08d9886123ebc2193d66ab6d1", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0024947FD0", "query_cov": 0.9193548387096774, "subject_cov": 0.8769230769230769, "identity": 0.7190000000000001, "score": 93.2, "evalue": 7.58e-24, "valid": false, "product": "peptide-methionine (R)-S-oxide reductase", "uniref50_id": "UniRef50_UPI0024947FD0" }, "pscc": { "uniref50_id": "UniRef50_UPI0024947FD0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0024947FD0" ], "product": "peptide-methionine (R)-S-oxide reductase" }, "genes": [], "id": "APKECPOBEN_1394", "locus": "GCA002090215_01394" }, { "type": "cds", "sequence": "contig_1", "start": 786432, "stop": 786635, "strand": "-", "frame": 2, "gene": "msrB", "product": "Peptide methionine sulfoxide reductase MsrB", "db_xrefs": [ "COG:COG0229", "COG:O", "EC:1.8.4.12", "GO:0005737", "GO:0006979", "GO:0030091", "GO:0033743", "SO:0001217", "UniRef:UniRef50_Q9CJ17", "UniRef:UniRef90_P75129" ], "nt": "ATGAGTAAGTACCAAAAAAAGAGTGACGGGGAACTAAAGCGGACCTTAACTAAACTACAGTACGACGTTACCCAAAACGCCCACACCGAACCGCCCTATACTAATGAATATAACCGCCATTACGAAAAGGGTATTTACGTTGACATTACGTCCGGTGAACCGTTGTTTATCTCAACTGATAAGTTCAAATCCGGTTGTGGTTGA", "aa": "MSKYQKKSDGELKRTLTKLQYDVTQNAHTEPPYTNEYNRHYEKGIYVDITSGEPLFISTDKFKSGCG", "aa_hexdigest": "e6168f0acf4796d952af691d466aa850", "start_type": "ATG", "rbs_motif": null, "genes": [ "msrB" ], "truncated": "5-prime", "pseudogene": { "start": 786180, "stop": 786635, "observations": { "mutation": [ 786434 ], "stop": [ 786434 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75129", "query_cov": 1.0, "subject_cov": 0.44370860927152317, "identity": 1.0, "score": 143.0, "evalue": 1.77e-42, "gene_start": 1, "gene_end": 67, "reference_start": 1, "reference_end": 67, "reference_sequence": "MSKYQKKSDGELKRTLTKLQYDVTQNAHTEPPYTNEYNRHYEKGIYVDITSGEPLFISTDKFKSGCGWPAFTKPISQDLIANYRDESHGMIRTEVRAKNSNSHLGHVFRDGPEEHGGLRYCINSAALKFIPFAEMESAGYGEYLKLFKESN" }, "paralog": false, "identity": 0.9933774834437086, "score": 311.0, "evalue": 2.52e-111, "description": "Internal stop codon at 786434. Nonsense mutation around 786434.", "psc": { "uniref90_id": "UniRef90_P75129", "gene": "msrB", "product": "Peptide methionine sulfoxide reductase MsrB", "ec_ids": [ "1.8.4.12" ], "uniref50_id": "UniRef50_Q9CJ17", "cog_id": "COG0229", "cog_category": "O", "go_ids": [ "GO:0005737", "GO:0033743", "GO:0030091", "GO:0006979" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75129", "EC:1.8.4.12", "UniRef:UniRef50_Q9CJ17", "COG:COG0229", "COG:O", "GO:0005737", "GO:0033743", "GO:0030091", "GO:0006979" ] }, "pscc": { "uniref50_id": "UniRef50_Q9CJ17", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9CJ17" ], "product": "Peptide methionine sulfoxide reductase MsrB" } }, "id": "APKECPOBEN_1395", "locus": "GCA002090215_01395" }, { "type": "cds", "sequence": "contig_1", "start": 786632, "stop": 787303, "strand": "-", "frame": 3, "gene": "ytpR", "product": "YtpR family tRNA-binding protein", "db_xrefs": [ "COG:COG0073", "COG:J", "GO:0000049", "SO:0001217", "UniRef:UniRef50_P47687", "UniRef:UniRef90_P75128" ], "nt": "ATGCGTTACGGTTTTTTTGATATTTCTCAGGACTTTATCACCATTTTTTGTCCCAAGAAAACACTTAAGAACTGTATGTTTGGTTTAATTGGTAGCCGCACGCAAGCTACTTTGCGCCAAGAAAAGAACCAGAACTTTAGCTTCTTTGTCAATGAAGCCAATGAGATTGCTGGGTTTAACTTCTTTGACATTAAAAAGAGCTTTCGCAGGGGATTAATTTCCCATCACTTTACCGCAGGGTTGAATTACCCTTCTTTGAAGTTGGTCAAAAAGATTAGTGAGCTGTTGAACTATGATTTAACACCCTTAGCCAAAAAGGTACCCTTTGTGGTTTGTGAGGTCATTTCGGCCATTCCGATTCCCAACACTCATTTGAAAAGGTGTAAGGTCAACACTGGTTCCAATAAGTCCTTGGATGTCGTGTGTGGGGCGGATAATGTTCGGGTTGGCCTTAAAACTGTTTTAGTCCATGTTGGTGGTGTTTTACCTGATGGCACGATTATCAAGAAGGCTAAAATTGCGGGGTATGATTCCATGGGAATGCTCTGTTCGGAAAAAGAGCTCAATTTAAAACCTAAAAATCAGGGTATAATTGAAATTAAGTCGCACATCAAAATAGGCAAAAGTTTCCTTGATGTTTACTTAAATAATAGTGAAAAATTCAGTGCATGA", "aa": "MRYGFFDISQDFITIFCPKKTLKNCMFGLIGSRTQATLRQEKNQNFSFFVNEANEIAGFNFFDIKKSFRRGLISHHFTAGLNYPSLKLVKKISELLNYDLTPLAKKVPFVVCEVISAIPIPNTHLKRCKVNTGSNKSLDVVCGADNVRVGLKTVLVHVGGVLPDGTIIKKAKIAGYDSMGMLCSEKELNLKPKNQGIIEIKSHIKIGKSFLDVYLNNSEKFSA", "aa_hexdigest": "f181a72f1f768b350ad8831b4930193a", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75128", "query_cov": 1.0, "subject_cov": 0.9529914529914529, "identity": 1.0, "score": 449.0, "evalue": 9.3e-160, "valid": true, "gene": "ytpR", "product": "YtpR family tRNA-binding protein", "uniref50_id": "UniRef50_P47687", "cog_id": "COG0073", "cog_category": "J", "go_ids": [ "GO:0000049" ] }, "pscc": { "uniref50_id": "UniRef50_P47687", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47687" ], "product": "Uncharacterized protein MG449" }, "genes": [ "ytpR" ], "id": "APKECPOBEN_1396", "locus": "GCA002090215_01396" }, { "type": "cds", "sequence": "contig_1", "start": 787310, "stop": 788182, "strand": "-", "frame": 3, "gene": "degV", "product": "DegV domain-containing MG450-like protein", "db_xrefs": [ "COG:COG1307", "COG:I", "GO:0008289", "RefSeq:WP_015344932.1", "SO:0001217", "UniParc:UPI0002B84ADF", "UniRef:UniRef100_P75127", "UniRef:UniRef50_P47688", "UniRef:UniRef90_P75127" ], "nt": "GTGAGAGTCGCATTCTTAGTTGACTCTGTTAGTAACCTCAAGGAAAGCCAGGCACAACATCTGTATGTGCTACCGCTTTTCATCATTGAATCAACTCCTGAAGCTGAAACCACCTTTAGTTCGGGGTTTGACATTGATTTAAAAACCCTCACGGACAAGATGGCTAACGCTCCGAAGGGAGTGAAGTTCTCCACATCGCAAACTACTGAAGAAATAGTGCGCGCTAAGGTGAGTGAACTAGTGGAGCAGTATGACCTGATTATCGGCATTCCGATTGATAAGGAAATCTCCGCTTCGTACGCGAACTGGAAGTTAGTTGAAAAGGATTACGCTTCGAAGTTCCATGTATTGGATGCCAGAGCGGTGGAAATGATCATTGATTGGCTAATCAATGACATTAAGGGTTGGTTGAACACCAATCCTTATTCCAGGGAAGGCTTGGATCGCTTTGTGGAGCAGTACCGCAAGAAAACGGCCGCAGTGTTGTTTGTCACTGATACCAAACCACTAGTGGCAGGCGGGCGGCTTTCCAATCTTAAATCCTTTATTATCAAGAGCTTAAAGTTTCACTTACTGATTAGTTTTTTAGGTGAAAATGGTAAGTTACAGTTCTTTGATAAAGCGCGTTCGGCTCATGATGCGCATAAGCTAGCAGTCAAGTTTTTAAAGAAACAGCTGTTAAAACACTCCGCTAAACTGAAACGGGGTGCCTTTCTTACCACCGTCTTTGACGAACAGACCAACACTAATTTAGTCCAAGAGTTTGATAAACTGTTGGATCATTCCATTAATATAGAACAGAGCTTACTGTCCCCGGTCATTTGTACCCACACGGGATTGAACAGCTATGTAATTGTGCTGCAAGGCGAATAG", "aa": "MRVAFLVDSVSNLKESQAQHLYVLPLFIIESTPEAETTFSSGFDIDLKTLTDKMANAPKGVKFSTSQTTEEIVRAKVSELVEQYDLIIGIPIDKEISASYANWKLVEKDYASKFHVLDARAVEMIIDWLINDIKGWLNTNPYSREGLDRFVEQYRKKTAAVLFVTDTKPLVAGGRLSNLKSFIIKSLKFHLLISFLGENGKLQFFDKARSAHDAHKLAVKFLKKQLLKHSAKLKRGAFLTTVFDEQTNTNLVQEFDKLLDHSINIEQSLLSPVICTHTGLNSYVIVLQGE", "aa_hexdigest": "c374d05a9635935e8a49a688f000b38d", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0002B84ADF", "ncbi_nrp_id": "WP_015344932.1", "uniref100_id": "UniRef100_P75127", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002B84ADF", "RefSeq:WP_015344932.1", "UniRef:UniRef100_P75127" ] }, "ips": { "uniref100_id": "UniRef100_P75127", "uniref90_id": "UniRef90_P75127" }, "psc": { "uniref90_id": "UniRef90_P75127", "gene": "DegV", "product": "DegV domain-containing protein MG450 homolog", "uniref50_id": "UniRef50_P47688", "cog_id": "COG1307", "cog_category": "I", "go_ids": [ "GO:0008289" ] }, "pscc": { "uniref50_id": "UniRef50_P47688", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47688" ], "product": "DegV domain-containing protein MG450" }, "genes": [ "degV" ], "id": "APKECPOBEN_1397", "locus": "GCA002090215_01397" }, { "type": "cds", "sequence": "contig_1", "start": 788182, "stop": 789366, "strand": "-", "frame": 1, "gene": "tuf", "product": "elongation factor Tu", "db_xrefs": [ "COG:COG0050", "COG:J", "GO:0001968", "GO:0003746", "GO:0003924", "GO:0005525", "GO:0005829", "GO:0006414", "GO:0009897", "GO:0016020", "RefSeq:WP_084253842.1", "SO:0001217", "UniParc:UPI000A043E20", "UniRef:UniRef100_UPI000A043E20", "UniRef:UniRef50_P23568", "UniRef:UniRef90_P23568" ], "nt": "ATGGCAAGAGAGAAATTTGACCGATCTAAACCCCACGTTAATGTAGGTACTATTGGCCACATTGACCACGGTAAAACAACTTTAACAGCAGCTATTTGTACTGTATTAGCAAAAGAAGGTAAATCAGCTGCTACTCGTTACGACCAAATCGATAAGGCTCCGGAAGAAAAAGCACGGGGAATTACGATTAACTCCGCTCACGTGGAGTACTCCTCTGACAAGCGTCACTATGCTCACGTTGACTGTCCAGGACACGCTGACTACATTAAGAACATGATTACTGGTGCTGCACAAATGGATGGTGCCATTCTAGTAGTTTCAGCAACTGACAGTGTTATGCCCCAAACCCGTGAACACATTTTGTTGGCCCGCCAAGTGGGTGTGCCACGCATGGTAGTGTTCCTAAACAAGTGTGACATTGCAACTGATGAAGAAGTGCAAGAGTTAGTAGCAGAAGAGGTACGTGACTTATTAACTTCTTACGGCTTTGATGGCAAGAACACCCCTATTATTTATGGTTCTGCACTTAAAGCGCTTGAAGGTGATCCTAAGTGGGAAGCTAAGATCCATGATTTAATGAATGCAGTTGATGAATGGATTCCAACTCCTGAACGTGAAGTGGACAAACCCTTCTTGTTGGCAATCGAAGACACCATGACGATTACTGGCCGTGGTACCGTGGTTACCGGTCGGGTTGAACGTGGTGAATTGAAAGTAGGTCAAGAAATTGAAATCGTTGGTTTACGTCCAATCCGTAAAGCAGTTGTTACCGGAATCGAAATGTTCAAAAAGGAACTTGATTCAGCAATGGCTGGGGACAACGCTGGGGTATTACTCCGTGGTGTGGACCGTAAAGAAGTGGAACGTGGTCAAGTGTTAGCTAAACCAGGTTCGATTAAACAGCACAAGAAATTTAAAGCGGAAATCTATGCTTTAAAGAAGGAAGAAGGTGGTCGTCACACCGGTTTCTTAAACGGTTACCGTCCCCAATTCTACTTCCGTACTACAGACGTTACTGGTTCGATTTCCCTACCAGAAAACACCGAAATGGTGCTACCAGGTGACAATACCTCGATTACAGTTGAACTAATTGCACCAATTGCTTGTGAAAAAGGTAGTAAGTTCTCCATCCGTGAAGGTGGTCGAACGGTTGGTGCTGGTTCAGTCACGGAAGTGCTTGAATAG", "aa": "MAREKFDRSKPHVNVGTIGHIDHGKTTLTAAICTVLAKEGKSAATRYDQIDKAPEEKARGITINSAHVEYSSDKRHYAHVDCPGHADYIKNMITGAAQMDGAILVVSATDSVMPQTREHILLARQVGVPRMVVFLNKCDIATDEEVQELVAEEVRDLLTSYGFDGKNTPIIYGSALKALEGDPKWEAKIHDLMNAVDEWIPTPEREVDKPFLLAIEDTMTITGRGTVVTGRVERGELKVGQEIEIVGLRPIRKAVVTGIEMFKKELDSAMAGDNAGVLLRGVDRKEVERGQVLAKPGSIKQHKKFKAEIYALKKEEGGRHTGFLNGYRPQFYFRTTDVTGSISLPENTEMVLPGDNTSITVELIAPIACEKGSKFSIREGGRTVGAGSVTEVLE", "aa_hexdigest": "8dd3046703e24cd08b8da8b3ddf0b88a", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000A043E20", "ncbi_nrp_id": "WP_084253842.1", "uniref100_id": "UniRef100_UPI000A043E20", "db_xrefs": [ "SO:0001217", "UniParc:UPI000A043E20", "RefSeq:WP_084253842.1", "UniRef:UniRef100_UPI000A043E20" ] }, "ips": { "uniref100_id": "UniRef100_UPI000A043E20", "uniref90_id": "UniRef90_P23568" }, "psc": { "uniref90_id": "UniRef90_P23568", "gene": "tuf", "product": "elongation factor Tu", "uniref50_id": "UniRef50_P23568", "cog_id": "COG0050", "cog_category": "J", "go_ids": [ "GO:0001968", "GO:0003746", "GO:0003924", "GO:0005525", "GO:0005829", "GO:0006414", "GO:0009897", "GO:0016020" ] }, "pscc": { "uniref50_id": "UniRef50_P23568", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P23568" ], "product": "Elongation factor Tu" }, "genes": [ "tuf" ], "id": "APKECPOBEN_1398", "locus": "GCA002090215_01398" }, { "type": "cds", "sequence": "contig_1", "start": 789517, "stop": 789912, "strand": "+", "frame": 1, "gene": null, "product": "putative MG452-like protein", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P75125", "UniRef:UniRef90_P75125" ], "nt": "TTGGGGGTAATTCCCTTATTTTTTATTTGTTTTGTTTTTGTAATTGCTGACATTGTCATCAGTTTGCAAAATAAAGGCCACATTATAGAAGAGATAGATAAGTTTACTAACCAGAGCAATGTTATGTTGCTCATTTATGCATGTTGGTATGTAAGCAAACCAAAGAGCCATTATCTCAAGAACCAGCAGTTCTTTTTGAGTGCCTTTGCTTACATCATCTTTACTTTTTTAGGCTACAACGTCATTTTGGCGGCTTCACAACAAGCTTACAGTGATAAGGATGCTTATTCCTTGGCAAGTTCTGTCTTTCTCCATGTGTTGGCACCAATTGCTTTCTTGGTTGCAGGTATAGTTAAGATGAAAACGGACAAGGATGTAACCTTTAATCATTTTTGA", "aa": "MGVIPLFFICFVFVIADIVISLQNKGHIIEEIDKFTNQSNVMLLIYACWYVSKPKSHYLKNQQFFLSAFAYIIFTFLGYNVILAASQQAYSDKDAYSLASSVFLHVLAPIAFLVAGIVKMKTDKDVTFNHF", "aa_hexdigest": "45aa88c1ce0d70b2c955d8260f249865", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 789451, "stop": 790206, "observations": { "mutation": [ 789481, 789910, 790072 ], "stop": [ 789481, 789910, 790072 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75125", "query_cov": 1.0, "subject_cov": 0.5219123505976095, "identity": 0.992, "score": 255.0, "evalue": 3.3e-84, "gene_start": 1, "gene_end": 131, "reference_start": 23, "reference_end": 153, "reference_sequence": "MEQNNCKIRTWFAKLALAQKVFLGVIPLFFICFVFVIADIVISLQNKGHIIEEIDKFTNQSNVMLLIYACWYVSKPKSHYLKNQQFFLSAFAYIIFTFLGYNVILAASQQAYSDKDAYSLASSVFLHVLAPIAFLVAGIVKMKTDKDVTFNHFWKSLGYFMIYPLVYGLYLATIPYVRGHYVSDDGKSTTYVVYGEITNTKDNPIVAWPVVICFLFIYFPLSFLAVYALQCKLLNRPLKAQFKCATNKCPK" }, "paralog": false, "identity": 0.9880478087649402, "score": 497.0, "evalue": 5.98e-182, "description": "Internal stop codon at 789481, 789910, 790072. Nonsense mutation around 789481, 789910, 790072.", "psc": { "uniref90_id": "UniRef90_P75125", "product": "Uncharacterized protein MG452 homolog", "uniref50_id": "UniRef50_P75125", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75125", "UniRef:UniRef50_P75125", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75125", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75125" ], "product": "Uncharacterized protein MG452 homolog" } }, "id": "APKECPOBEN_1399", "locus": "GCA002090215_01399" }, { "type": "cds", "sequence": "contig_1", "start": 789931, "stop": 790074, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATCTATCCCTTAGTGTATGGGTTGTACTTAGCGACCATTCCTTATGTAAGGGGTCATTATGTGAGTGATGATGGTAAGTCTACTACCTATGTGGTTTATGGGGAGATTACCAACACCAAGGATAACCCGATCGTCGCTTGA", "aa": "MIYPLVYGLYLATIPYVRGHYVSDDGKSTTYVVYGEITNTKDNPIVA", "aa_hexdigest": "785f9afb9451ddad465ab75d45e2a03a", "start_type": "ATG", "rbs_motif": "AAAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5271.944600000002, "isoelectric_point": 5.4105833053588865 }, "id": "APKECPOBEN_1400", "locus": "GCA002090215_01400" }, { "type": "cds", "sequence": "contig_1", "start": 790194, "stop": 790730, "strand": "+", "frame": 3, "gene": null, "product": "UTP--glucose-1-phosphate uridylyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3D0M420" ], "nt": "ATGCCCAAAATAAGAAAAGCCGTTATTCCCGCAGCCGGACTGGGTACCAGATTACTCCCAGCCACTAAGGCCATTCCCAAAGAGATGTTGCCCTTAGTCAATAAACCCACAATTCAGTACATTGTGGAAGAAGCAGTAGCGAGTGGCATTAAGGAAATCCTAGTAATTGTGTCTTCTAAAAAGGAAGCGATTATTGACCACTTTGACTATGACTTTATCTTGGAAAATGCTTTACTCCAAAAGCACAAAGATCAAGAACACCAAGAAATTAAGGATATTGCCAACTTAGCGCACATTTACTTTGTCCGCCAAAAACACCAACACGGTTTAGGAGATGCCATTTTGCACGCCAAGTCGTTTGTTGGTAACGAAGACTTTGCCGTCCTTTTAGGTGATGATGTCGTGTTTGGTGAACAACCGGCATTAGCACAATGTATTCAAGCTTATGAACAAACAGATTGTCAGGTAATAGGCGTGCAAGAAGTGCCCCATGACCAGGTCAACAAGTATGGCATAGTAACCCCTGAAGCTAATTGA", "aa": "MPKIRKAVIPAAGLGTRLLPATKAIPKEMLPLVNKPTIQYIVEEAVASGIKEILVIVSSKKEAIIDHFDYDFILENALLQKHKDQEHQEIKDIANLAHIYFVRQKHQHGLGDAILHAKSFVGNEDFAVLLGDDVVFGEQPALAQCIQAYEQTDCQVIGVQEVPHDQVNKYGIVTPEAN", "aa_hexdigest": "80fdc0b8a704ba45efd13621b614de99", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0020C167D8", "query_cov": 0.9831460674157303, "subject_cov": 0.9259259259259259, "identity": 0.589, "score": 199.0, "evalue": 2.2e-62, "valid": false, "product": "UTP--glucose-1-phosphate uridylyltransferase", "ec_ids": [ "2.7.7.9" ], "uniref50_id": "UniRef50_A0A3D0M420", "kegg_orthology_id": "K00963" }, "pscc": { "uniref50_id": "UniRef50_A0A3D0M420", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3D0M420" ], "product": "UTP--glucose-1-phosphate uridylyltransferase" }, "genes": [], "id": "APKECPOBEN_1401", "locus": "GCA002090215_01401" }, { "type": "cds", "sequence": "contig_1", "start": 790761, "stop": 791069, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTGGAAAAACCAGCTGTCAACGAAGCCAAGAGCAACCTAGCCATTTTGAGCCGTTACATCCTTAAACCCAGTATTTTTACAGCTCTTAAACAAGTACCGTTTGGTGTTGGGGGTGAGTTACAGTTGACTGATGGGTTGAACTATTGTTTACAGCAAGGGGAACCCTTCTTTGCCAAGCACTTTGGTGGTACCCGGTTTGATGTCGGTACTAAAAATGGTTTTATTAAAGCGAACCTGTACACGGCTTTAAAAACCGATGCCATTACTAAGGATGAAGTCTTGGCCATCCTCAAGGAATTTGCTTAA", "aa": "MVEKPAVNEAKSNLAILSRYILKPSIFTALKQVPFGVGGELQLTDGLNYCLQQGEPFFAKHFGGTRFDVGTKNGFIKANLYTALKTDAITKDEVLAILKEFA", "aa_hexdigest": "bf0631771f763e0b86637096c7b8d18b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11181.844799999984, "isoelectric_point": 8.733677101135253 }, "id": "APKECPOBEN_1402", "locus": "GCA002090215_01402" }, { "type": "cds", "sequence": "contig_1", "start": 791066, "stop": 791488, "strand": "-", "frame": 3, "gene": "osmC", "product": "Organic hydroperoxide resistance protein-like", "db_xrefs": [ "COG:COG1764", "COG:V", "EC:1.11.1.28", "GO:0006979", "KEGG:K04063", "RefSeq:WP_010875025.1", "SO:0001217", "UniParc:UPI0000139829", "UniRef:UniRef100_P75123", "UniRef:UniRef50_P47692", "UniRef:UniRef90_P75123" ], "nt": "ATGGCAGTAATTTACAAAACAACAGCACACGCTAGCGCCGGCAGGGAAGGCGTTGTCCAAACCGTTGATGGTTTTACCGTTAGTTTAGCATTTCCTAAACCTGGTGCTACTCACCAGGACAAAAACAACCCCGAACAGCTGTTTGCTTCAGCTTATGCTGGTTGTTTTTCGCAAGCTGTGCGTGTGGTTTTACAACAGCACCAACTGCAATTAGCTACTCAACCTATTGTGGGTGTTAGTGTCGAACTGCATGACCAGGACGGTCTGTTCCACATTAAAGCCGGTGTTGAATTAGCGATTACGGGCGTGGACCAAACCACAGCGCAAACAGTCATTACGGCAGCGCATGCCATGTGTCCGTTTAGCCGTTTAATTAAACCAGAAAACTTCTTGGGTTTAACCTTGAACGGTGCCAAGCTTTAA", "aa": "MAVIYKTTAHASAGREGVVQTVDGFTVSLAFPKPGATHQDKNNPEQLFASAYAGCFSQAVRVVLQQHQLQLATQPIVGVSVELHDQDGLFHIKAGVELAITGVDQTTAQTVITAAHAMCPFSRLIKPENFLGLTLNGAKL", "aa_hexdigest": "b94a5980c1a9ce9401afd5a7f15cdac8", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000139829", "ncbi_nrp_id": "WP_010875025.1", "uniref100_id": "UniRef100_P75123", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139829", "RefSeq:WP_010875025.1", "UniRef:UniRef100_P75123" ] }, "ips": { "uniref100_id": "UniRef100_P75123", "uniref90_id": "UniRef90_P75123" }, "psc": { "uniref90_id": "UniRef90_P75123", "gene": "OsmC", "product": "Organic hydroperoxide resistance protein-like", "ec_ids": [ "1.11.1.28" ], "uniref50_id": "UniRef50_P47692", "cog_id": "COG1764", "cog_category": "V", "kegg_orthology_id": "K04063", "go_ids": [ "GO:0006979" ] }, "pscc": { "uniref50_id": "UniRef50_P47692", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47692" ], "product": "Organic hydroperoxide resistance protein-like" }, "genes": [ "osmC" ], "id": "APKECPOBEN_1403", "locus": "GCA002090215_01403" }, { "type": "cds", "sequence": "contig_1", "start": 791514, "stop": 792713, "strand": "+", "frame": 3, "gene": "tyrS", "product": "tyrosine--tRNA ligase", "db_xrefs": [ "COG:COG0162", "COG:J", "EC:6.1.1.1", "GO:0000166", "GO:0003723", "GO:0004831", "GO:0005524", "GO:0005829", "GO:0006437", "KEGG:K01866", "RefSeq:WP_010875026.1", "SO:0001217", "UniParc:UPI0000136767", "UniRef:UniRef100_P75122", "UniRef:UniRef50_P47693", "UniRef:UniRef90_P75122" ], "nt": "ATGACAAAAGATCTCCTAGCACTTTTAAAAGAACGGGGCTTATTTGTACAAGCTAACTTTGAAAAGGAATTAAAGCAATTATTAAACCAAGGATCATTTGCTTTCTATGTTGGCTTTGACCCCACAGCACCCTCACTCCATATTGGCAACTATGTGCTGCTCCATGTAGCACAAATCTTCCAAGCAATGGGTCATATTCCCCACGTCTTGTTGGGTAGTGGGACGGCTTTAATAGGTGATCCTACTGGCAGAATGGAACTGCGCCAGATGATGAGTCGTGAAACAATTGCCGAAAATACGCGCAACATTAAAAAACAAATTAGGCGCTTTTTGGGTAGTAATGTAGTGTTTTGCCAAAACGAGACCTGGTTAAAGAAACTCAATTACATTGAGGTCATCAGGGAATTGGGACCCTGCTTTTCGGTCAACAAGATGTTAGCGACCGATGCCTTTAGTGCTCGTTGGGAACGCGGTTTAACGTTGATGGAATTGAACTACATGGTATTACAGGCCTATGACTTCTATTATTTAAACCAAAAGTATGGGGTGCAACTGCAAATAGGGGGCAGTGACCAGTGGGCTAATATCCTAGCGGGGGCTGATTTAATTAGACGCAAAACCCAAAAACAGGTGTACGGCATGACCACTAACTTATTAGTGAAAGCTAATGGGGAAAAGATGGGTAAGAGTGCTAGTGGAGCTTTATGGTTAGATCCACAAAAGACCAGTCCGTATGACTTCTACCAGTATTGGATTAACCTTGATGATGCTAGTTTGCAAAAGGTTTTCCTTATGCTCACTAAATTAGATACCAAGGCAATTGAAACCCTCTGCAACTTGAAGGGCGCTGCCATTAAGGAGGCCAAGGCAAAACTAGCCTTCGAGTTAACTGACGCTATTCATGGTACCAAGGCAGCTTTAGTCGCCCAAGCGAAAAGTGCCCGAATCTTTGCCTTTCAACCTGATACTGAAACCAAAACCGTGCGAGCTGGCACCAGGTTAGTGGATGTGATTGTTGATTTAGGTTTAGTTGTATCCAGATCAGAAGCGCGCCGGGTGATTCAGCAAGGTGGTTTAACGATTAACCAAGAAAAAGTGACTGATGTGGAAATGGTCCTACAAGCGAGTTCCCAACCACTAGTGATTGGTAAGGGCAAAAAGCGTTTTGTAACGGTGCAAGTGATAGCTAACACCAAATAA", "aa": "MTKDLLALLKERGLFVQANFEKELKQLLNQGSFAFYVGFDPTAPSLHIGNYVLLHVAQIFQAMGHIPHVLLGSGTALIGDPTGRMELRQMMSRETIAENTRNIKKQIRRFLGSNVVFCQNETWLKKLNYIEVIRELGPCFSVNKMLATDAFSARWERGLTLMELNYMVLQAYDFYYLNQKYGVQLQIGGSDQWANILAGADLIRRKTQKQVYGMTTNLLVKANGEKMGKSASGALWLDPQKTSPYDFYQYWINLDDASLQKVFLMLTKLDTKAIETLCNLKGAAIKEAKAKLAFELTDAIHGTKAALVAQAKSARIFAFQPDTETKTVRAGTRLVDVIVDLGLVVSRSEARRVIQQGGLTINQEKVTDVEMVLQASSQPLVIGKGKKRFVTVQVIANTK", "aa_hexdigest": "ffc02f922f0387f48598d1eff0a60063", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000136767", "ncbi_nrp_id": "WP_010875026.1", "uniref100_id": "UniRef100_P75122", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000136767", "RefSeq:WP_010875026.1", "UniRef:UniRef100_P75122" ] }, "ips": { "uniref100_id": "UniRef100_P75122", "uniref90_id": "UniRef90_P75122" }, "psc": { "uniref90_id": "UniRef90_P75122", "gene": "tyrS", "product": "tyrosine--tRNA ligase", "ec_ids": [ "6.1.1.1" ], "uniref50_id": "UniRef50_P47693", "cog_id": "COG0162", "cog_category": "J", "kegg_orthology_id": "K01866", "go_ids": [ "GO:0000166", "GO:0003723", "GO:0004831", "GO:0005524", "GO:0005829", "GO:0006437" ] }, "pscc": { "uniref50_id": "UniRef50_P47693", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47693" ], "product": "Tyrosine--tRNA ligase" }, "genes": [ "tyrS" ], "id": "APKECPOBEN_1404", "locus": "GCA002090215_01404" }, { "type": "cds", "sequence": "contig_1", "start": 792753, "stop": 793790, "strand": "-", "frame": 2, "gene": null, "product": "putative MG456-like protein", "db_xrefs": [ "GO:0005886", "RefSeq:WP_010875027.1", "SO:0001217", "UniParc:UPI0000139830", "UniRef:UniRef100_P75121", "UniRef:UniRef50_P47694", "UniRef:UniRef90_P75121" ], "nt": "ATGCCAGAGCTAACTCGTTTTCAAAAGTTCTTTTTAACACCTGAAAAGTTCAATAAATTTACAAGAGTTGTTGGTTTTTGTGGTGTCTTTGCTTTAATTGCCCTTTCTTTAGGGATTTACAGCTATGTTGGTCAGGGTTCAATTGTTCCTAAAGTGGCAGCTCTTTTCCTAATTGCCTTAGGCGGGTTTACCTTGCTGTTGAGCTTTGTGATTAACTTTGTAGCTTTGTACAAACGCAGTCAGTTAATTCATTTGGTCAACCGTCAAGATCGTACTGATTTGTGGTTACAAAAGATGGCAAACAACAAGCAGTTTGAGCAATTTGAATTGTTTGAAAAAGGTCCAATTAGTGCGGATATTCTACCGACTTTTTACCCTGCTACCATCTATAACTTTGAGTTAGTTCCCAAGCAATTTAAGGTACAGTACAAGAATGGTCAAACGCTTAACTTTGCTAAGTTAAGTGCCATTAAGAGAAGTACTTCCAAAAATGAAAAGGTGGCTTGCTTAGTCGCTATTATTGATGCTGTTAGTGACCAACACTGGTTCTTAACTAAAAGCGATTTCCCCTTAATTAACACTGGTTTTTACGAATCTTTAACTGAAAGTAACCGCCAGAACAACGTTTTGCTCTACACTGAAAAAGACGCTAGTTTTAACTTTAATCAGTTAGATAAGGAGATGATAAAGCAGGTGTTGTTTAACCCGGTTAATGTGTATGCTAACTTTAACGTTTACAACAATACCACACACACTTACCTCATGATGAGTGTACCGATTACCTTCATGGATACTTCTTTAAGAATGGAGGAAGCTGTGGGTGATTTAGAACTCAATATTACCCGTCAAGCTGGTTATGACGCTGCTACCTTAGATTCATTCCATAAGGTAGTGGAACTGTTAAAGACCAAACTCATTGGTGATTTCAACAACGCTGAAACTACCAGTGCAACAGAAACAACAGTTGTGGCTGAAGTTACTGAACCCACAACCAATTCCAAGCGCAAACCAGTTAAAGCGAAGAAGGCTAAGAAATAA", "aa": "MPELTRFQKFFLTPEKFNKFTRVVGFCGVFALIALSLGIYSYVGQGSIVPKVAALFLIALGGFTLLLSFVINFVALYKRSQLIHLVNRQDRTDLWLQKMANNKQFEQFELFEKGPISADILPTFYPATIYNFELVPKQFKVQYKNGQTLNFAKLSAIKRSTSKNEKVACLVAIIDAVSDQHWFLTKSDFPLINTGFYESLTESNRQNNVLLYTEKDASFNFNQLDKEMIKQVLFNPVNVYANFNVYNNTTHTYLMMSVPITFMDTSLRMEEAVGDLELNITRQAGYDAATLDSFHKVVELLKTKLIGDFNNAETTSATETTVVAEVTEPTTNSKRKPVKAKKAKK", "aa_hexdigest": "5c92a724c8cf4ee52878013d9ef9b424", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000139830", "ncbi_nrp_id": "WP_010875027.1", "uniref100_id": "UniRef100_P75121", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139830", "RefSeq:WP_010875027.1", "UniRef:UniRef100_P75121" ] }, "ips": { "uniref100_id": "UniRef100_P75121", "uniref90_id": "UniRef90_P75121" }, "psc": { "uniref90_id": "UniRef90_P75121", "product": "Uncharacterized protein MG456 homolog", "uniref50_id": "UniRef50_P47694", "go_ids": [ "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47694", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47694" ], "product": "Uncharacterized protein MG456" }, "genes": [], "id": "APKECPOBEN_1405", "locus": "GCA002090215_01405" }, { "type": "cds", "sequence": "contig_1", "start": 793778, "stop": 794359, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_015344934.1", "SO:0001217", "UniParc:UPI0002B849E8", "UniRef:UniRef100_UPI0002B849E8", "UniRef:UniRef50_A0AAX0SQR6", "UniRef:UniRef90_A0AAX0SQR6" ], "nt": "GTGAAACAAACAGTTAAGTTTGCTAGTTTTGTCGATCAGCTCCTAGTTAACCTCGCGCACTTGCAAAAGCAGCTCAACCACTTGCAACCCGATTTGGGCACCTATAGCTTTCAGTGTTTTACCACCCTCAACGATGTTGATAAAACCCCGCAAAACTTTATTGCAGTCTTAAAGCAACACTCCAACTTGTTGGGTTGTGAAACCAATGCTGTGCTAACTTGGTGGGAGGCTGTAGAATACCTGTTGTTAGAAGAAAACAAGGATGTGATTACCGTGGTGAAAACCCTTGTTAAACACACCGAAAAATTGATACAAACTAAAGCAATTACTTTAGGTCACAAACGGGTGTGGGATTTGTTGTATTACTTTTGTAAAAGCTTCTTGCAATTAGTAAACCGCACCAAAAGGCAGTACAAGCAGCTCGATGGGGTGTTAGTTGAACTGTATGAACGGCTTTGTCAAACCCGGGCGCAGTTTGGCAAGGCGCAGTTTTTAGGGAAACGTTCTGTAGGGCAAACAACTCCAGCGTTGTGTTTAACTTATCTTTTCTTACAATCTTTTATATACGCATGCCAGAGCTAA", "aa": "MKQTVKFASFVDQLLVNLAHLQKQLNHLQPDLGTYSFQCFTTLNDVDKTPQNFIAVLKQHSNLLGCETNAVLTWWEAVEYLLLEENKDVITVVKTLVKHTEKLIQTKAITLGHKRVWDLLYYFCKSFLQLVNRTKRQYKQLDGVLVELYERLCQTRAQFGKAQFLGKRSVGQTTPALCLTYLFLQSFIYACQS", "aa_hexdigest": "b3a7fb326fe2149ff03a1fff09249483", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0002B849E8", "ncbi_nrp_id": "WP_015344934.1", "uniref100_id": "UniRef100_UPI0002B849E8", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002B849E8", "RefSeq:WP_015344934.1", "UniRef:UniRef100_UPI0002B849E8" ] }, "ips": { "uniref100_id": "UniRef100_UPI0002B849E8", "uniref90_id": "UniRef90_A0AAX0SQR6" }, "psc": { "uniref90_id": "UniRef90_A0AAX0SQR6", "uniref50_id": "UniRef50_A0AAX0SQR6" }, "pscc": { "uniref50_id": "UniRef50_A0AAX0SQR6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAX0SQR6" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 22371.794200000004, "isoelectric_point": 9.097407722473147 }, "id": "APKECPOBEN_1406", "locus": "GCA002090215_01406" }, { "type": "cds", "sequence": "contig_1", "start": 794343, "stop": 795800, "strand": "-", "frame": 2, "gene": "ftsH", "product": "ATP-dependent zinc metalloprotease FtsH", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A357JCK8" ], "nt": "GTGAGGTTTGACAACATTGCGGGACTCCAAGAAGAAAAACACGAGTTACTGGAAATTGTTGATTACCTCAAAAACCCCTTGAAGTATGCCCAAATGGGCGCTCGTTCCCCACGCGGTGTTATTCTTTACGGCCCTCCTGGTACGGGTAAAACGCTGTTAGCGAAAGCTGTTGCTGGTGAAGCTGGTGTGCCTTTCTTCCAGTCCACGGGTTCTGGCTTTGAAGATATGCTGGTTGGTGTGGGCGCCAAACGGGTACGGGACTTGTTCAATAAGGCTAAGAAAGCGGCCCCATGTATTATCTTCATCGATGAAATTGACTCGGTTGGTTCTAAACGGGGTCGCGTGGAGTTGTCCTCCTACTCGGTGGTCGAACAAACCTTAAACCAACTGTTAGCGGAAATGGATGGGTTTACTAGCCGCACTGGTGTTGTGGTGATGGCTGCTACCAACCGCTTGGATGTCCTTGATGATGCGTTATTACGCCCAGGTCGGTTTGATCGTCACATCCAGATTAACCTTCCCGACATTAAGGAACGCGAAGGCATCTTACAAGTGCACGCCAAGAACAAGAACTTGTCTTCCAAGATTAGCTTATTGGATGTTGCTAAACGTACCCCTGGTTTTAGTGGGGCTCAATTGGAAAACGTCATTAATGAAGCTACCCTGTTAGCAGTCCGTGATAACCGGACCACGATCAACATGAACGACATTGATGAAGCCATTGACCGGGTCATAGCTGGTCCTGCCAAAAAATCTCGGGTGGTCAGTGATGCTGATCGTAAACTAGTGGCCTACCACGAAGCAGGTCATGCTTTGGTTGGCCTCCATGTTCACAGTAACGATGAAGTGCAAAAGATTACCATTATTCCCCGGGGTCAAGCGGGTGGTTATACCTTGTCAACCCCTAAGAGTGGCGATCTCAATTTAAAGCGTAAATCTGACTTGTTAGCGATGATTGCTACAGCCATGGGCGGCCGTGCTGCAGAGGAAGAAATTTACGGTCCCCTAGAAATTACCACTGGCGCTTCCTCGGACTTTTATAAGGCGACCAATATTGCCCGTGCCATGGTTACCCAGTTAGGAATGTCCAAGTTAGGGCAAGTGCAATATGTACCTAGTCAAGGTACCGTCCCACCAGGTACCAAACTGTTCTCTGAGCAAACAGCAAAGGATATTGACTTTGAGATTAACGCCATTATTGAAGAACAGTACAAGAAGGCACGTACCATTATTAAAACGAACCGTAAGGAGTTGGAATTGTTAGTTGAAGCCTTGTTGATTGCCGAAACCATCTTAAAGAGTGACATCGATTACATTCACGAACACACTAAACTCCCTCCAGAGATCCTAGCGCAAAAGCAAGAACAGCAAGCCAAACAAAAGGCAGAAGCCAAGGAAGCTAAGTTAAATAAGAAGACTGAGAAGGATACAGAAAAAGACAGTGAAACAAACAGTTAA", "aa": "MRFDNIAGLQEEKHELLEIVDYLKNPLKYAQMGARSPRGVILYGPPGTGKTLLAKAVAGEAGVPFFQSTGSGFEDMLVGVGAKRVRDLFNKAKKAAPCIIFIDEIDSVGSKRGRVELSSYSVVEQTLNQLLAEMDGFTSRTGVVVMAATNRLDVLDDALLRPGRFDRHIQINLPDIKEREGILQVHAKNKNLSSKISLLDVAKRTPGFSGAQLENVINEATLLAVRDNRTTINMNDIDEAIDRVIAGPAKKSRVVSDADRKLVAYHEAGHALVGLHVHSNDEVQKITIIPRGQAGGYTLSTPKSGDLNLKRKSDLLAMIATAMGGRAAEEEIYGPLEITTGASSDFYKATNIARAMVTQLGMSKLGQVQYVPSQGTVPPGTKLFSEQTAKDIDFEINAIIEEQYKKARTIIKTNRKELELLVEALLIAETILKSDIDYIHEHTKLPPEILAQKQEQQAKQKAEAKEAKLNKKTEKDTEKDSETNS", "aa_hexdigest": "16d13bd606ae783af3b7d49c732ab51c", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3D5RJK9", "query_cov": 0.9237113402061856, "subject_cov": 0.8327137546468402, "identity": 0.5579999999999999, "score": 473.0, "evalue": 3.3e-160, "valid": false, "gene": "ftsH", "product": "ATP-dependent zinc metalloprotease FtsH", "uniref50_id": "UniRef50_A0A357JCK8", "go_ids": [ "GO:0004222", "GO:0006508", "GO:0016020", "GO:0016887", "GO:0051301" ] }, "pscc": { "uniref50_id": "UniRef50_A0A357JCK8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A357JCK8" ], "product": "ATP-dependent zinc metalloprotease FtsH" }, "genes": [ "ftsH" ], "id": "APKECPOBEN_1407", "locus": "GCA002090215_01407" }, { "type": "cds", "sequence": "contig_1", "start": 795894, "stop": 796385, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGTTATCCTAGCCATTATTATTGGTATTTTGGTTTATATCCTAATGCCAAGGGCTACCACGGCTGTAATTGAAAAGTGGGAGCTTAGTGGAACTACACTTTCGGCTCAAATCAAAGGCTTATCAGGCAAACACACCTTCCAAAGAATTAATAATTCCACCTATGTCACCGATGACATTCTGCAGGTTTCCATTAGTTTCCAAGGGATAAATCCCATAGTAGTAACTGCCCACAAAGCTACTAATGGTAGTGGGGAAACTATCTTTAATATAGCAAACCTCTCGATTAATCAGTCAACCGGAAAGGCCATAGTTAATGGAATGATGACCCAAGACCAAAAAAGTAACAATGGCACTGAATTAGCTTCTATTAAAGGTCTCCATGACATCGGTACCTTTGTTGCTCCTGATATGAGAGCACGTGATGTGCTCAACATCTTCTTTGGCCTACTACCAATTATTATCTTTGTCATTTTCTTCTTACTCTTTTGA", "aa": "MVILAIIIGILVYILMPRATTAVIEKWELSGTTLSAQIKGLSGKHTFQRINNSTYVTDDILQVSISFQGINPIVVTAHKATNGSGETIFNIANLSINQSTGKAIVNGMMTQDQKSNNGTELASIKGLHDIGTFVAPDMRARDVLNIFFGLLPIIIFVIFFLLF", "aa_hexdigest": "cff2596e72640ef1c03aced95719f4bf", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17739.54669999999, "isoelectric_point": 8.068298912048338 }, "id": "APKECPOBEN_1408", "locus": "GCA002090215_01408" }, { "type": "cds", "sequence": "contig_1", "start": 796653, "stop": 797180, "strand": "-", "frame": 2, "gene": "hpt", "product": "hypoxanthine phosphoribosyltransferase", "db_xrefs": [ "COG:COG0634", "COG:F", "EC:2.4.2.8", "GO:0000166", "GO:0000287", "GO:0004422", "GO:0005829", "GO:0006166", "GO:0006178", "GO:0032263", "GO:0032264", "GO:0046100", "GO:0052657", "KEGG:K00760", "RefSeq:WP_010875029.1", "SO:0001217", "UniParc:UPI000012CB66", "UniRef:UniRef100_P75119", "UniRef:UniRef50_P47696", "UniRef:UniRef90_P75119" ], "nt": "ATGGGCATTAAATCTATCATCATTGATCAAAAACAGGTTGAAGCGGGTTGTAACGCTGCGCTCAAATGGTGTAATGAACATTTTGCTGGTAAACAGGTCATAGTATTAGGGATCTTGAAGGGTTGTATTCCCTTTTTGGGCAAACTTATTAGTCAGTTTACCTTTGATTTACAACTGGACTTTGTGGCAGTCGCTTCTTACCATGGTGGCAGTCGCCAACAAGAAGCACCGAAGATTGTCCTAGATATGTCCCATGACCCTAAAGGCAAGGACATTCTTTTAATAGAAGACATAGTCGACAGTGGTCGTTCTATTAAATTAGTATTGGACTTACTCCACACCCGAAAGGCCAAAAGTGTGATCTTAGTGAGCTTCATTGAAAAGCTCAAACCCAGGGAAGCTGACATTAAGGTGGACTATTCTTGCTTTAAAAACCAAGACGAATTCTTGGTTGGTTTTGGGTTAGACTACCAGGGCTTTTATCGCAATTTACCCTATGTTGGTGTCTTTGATCCTGAAGATAATTAA", "aa": "MGIKSIIIDQKQVEAGCNAALKWCNEHFAGKQVIVLGILKGCIPFLGKLISQFTFDLQLDFVAVASYHGGSRQQEAPKIVLDMSHDPKGKDILLIEDIVDSGRSIKLVLDLLHTRKAKSVILVSFIEKLKPREADIKVDYSCFKNQDEFLVGFGLDYQGFYRNLPYVGVFDPEDN", "aa_hexdigest": "aa34081ce838239386bd64d03af60595", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000012CB66", "ncbi_nrp_id": "WP_010875029.1", "uniref100_id": "UniRef100_P75119", "db_xrefs": [ "SO:0001217", "UniParc:UPI000012CB66", "RefSeq:WP_010875029.1", "UniRef:UniRef100_P75119" ] }, "ips": { "uniref100_id": "UniRef100_P75119", "uniref90_id": "UniRef90_P75119" }, "psc": { "uniref90_id": "UniRef90_P75119", "gene": "hpt", "product": "hypoxanthine phosphoribosyltransferase", "ec_ids": [ "2.4.2.8" ], "uniref50_id": "UniRef50_P47696", "cog_id": "COG0634", "cog_category": "F", "kegg_orthology_id": "K00760", "go_ids": [ "GO:0000166", "GO:0000287", "GO:0004422", "GO:0005829", "GO:0006166", "GO:0006178", "GO:0032263", "GO:0032264", "GO:0046100", "GO:0052657" ] }, "pscc": { "uniref50_id": "UniRef50_P47696", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47696" ], "product": "Hypoxanthine-guanine phosphoribosyltransferase" }, "genes": [ "hpt" ], "id": "APKECPOBEN_1409", "locus": "GCA002090215_01409" }, { "type": "cds", "sequence": "contig_1", "start": 797180, "stop": 797605, "strand": "-", "frame": 3, "gene": "ispF", "product": "putative MG459-like protein", "db_xrefs": [ "COG:COG0245", "COG:I", "GO:0008685", "GO:0016114", "SO:0001217", "UniRef:UniRef50_Q49436", "UniRef:UniRef90_P75118" ], "nt": "GTGGAAATAGAAGATTCCAAGTACATTTATGACCACGATGCTAGTGATGACGCCAGTGACGTTATCCAGCTAGCAGTAGCTGATGCTTTATTTGGTGCTACTGCCTTAGGTGATGGACAAATTGTCTTCAATAAAGAAAAAACCCAGATCCCTTTAAAGGGTAATCCGCGTAAAGAAGCGCCCCGTATTTTGGCACGGACTTACAACTTTATTCGCAAGAACTGGTACATCAATAACATTGATATCACCTTAGAAATTCCCTCACAACAAAAGATGGATGACTATAAGCATGCCATCTTTGACTTTATTTGTACGGCTTTACGGATTACCGAACTGACAATTAACCTCAAGGTACGTGAACCGTTGAATCCGAATGAAATTAGTTGTTTAGCGGTCGTGTTAGTGGAACGACAAAGATTAAAGTAA", "aa": "MEIEDSKYIYDHDASDDASDVIQLAVADALFGATALGDGQIVFNKEKTQIPLKGNPRKEAPRILARTYNFIRKNWYINNIDITLEIPSQQKMDDYKHAIFDFICTALRITELTINLKVREPLNPNEISCLAVVLVERQRLK", "aa_hexdigest": "32def9018c861a8f14d0018ce4450556", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75118", "query_cov": 1.0, "subject_cov": 0.834319526627219, "identity": 0.993, "score": 278.0, "evalue": 1.61e-94, "valid": true, "gene": "IspF", "product": "Uncharacterized protein MG459 homolog", "uniref50_id": "UniRef50_Q49436", "cog_id": "COG0245", "cog_category": "I", "go_ids": [ "GO:0008685", "GO:0016114" ] }, "pscc": { "uniref50_id": "UniRef50_Q49436", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49436" ], "product": "Uncharacterized protein MG459" }, "genes": [ "ispF" ], "id": "APKECPOBEN_1410", "locus": "GCA002090215_01410" }, { "type": "cds", "sequence": "contig_1", "start": 797806, "stop": 798798, "strand": "+", "frame": 1, "gene": "ldh", "product": "L-lactate dehydrogenase", "db_xrefs": [ "COG:C", "COG:COG0039", "EC:1.1.1.27", "GO:0004459", "GO:0005737", "GO:0006089", "GO:0006096", "GO:0016020", "KEGG:K00016", "SO:0001217", "UniParc:UPI0001DD6490", "UniRef:UniRef100_P78007", "UniRef:UniRef50_P78007", "UniRef:UniRef90_P78007" ], "nt": "ATGATTGGTGGCTTAAACCAAATTAAAATGAAGGCAAGCTTGAATAAATTAGTTATGAAGAGTCTTAAAGTAGCACTCATTGGTTCTGGTGCAGTGGGCACCAGCTTTCTCTACGCTGCCATGAGCCGTGGTTTAGCGAGTGAATACATGGTTATCGACATTAACGAAAAATCCCAAGTAGGGAATGTTTTTGACCTCCAAGATGCTGTACCATCATCACCGCAATACAGTAAGGTAATTGCTGGTGATTACAAACAACTCAAAGATTACGACTTTATCTTTATTGGTGCTGGTCGACCCCAAAAGCAAGGTGGGGAAACCCGGTTACAACTCCTTGAAGGTAATGTCGAAATTATGAAGAACATTGCCAAAGCCGTGAAGGAATCGGGCTTTAAGGGCATTACCTTAATTGCTTCCAACCCAGTCGACATTATGGCTTACACCTATTTAAAGGTAACGGGGTTTGAACCGAATAAGGTAATTGGTTCTGGAACCTTACTAGACAGCGCCCGGTTAAAGTTTGCGATTGCTGAAAAATATGGTATGAGCAGCAGGGATGTCCAAGCATATGTGTTAGGTGAACACGGGGATAGTTCAGTAAGTATTATTTCCTCCGCTAAGATTGCTGGCTTACCACTCAAACACTTCTCGAAAGCAAGTGATATAGAAAAAGAATTTGCTGAAATTGACCACTTTATTCGCCGTCGAGCTTATGAAATCATCGAACGCAAAGGAGCCACCTTTTATGGCATCGGGGAAGCAACTGCCGAAGTAGCAGAATTAATTCTCCGCGACACTAAAGAAGTACGAGTAGTAGCATCGTTAATTAATGGCCAATATGGTGCTAAAGATGTAATGTTTGGCACCCCTTGTGTCTTGGGACGCAATGGTGTGGAAAAGATCTTGGAAATTGAATTGTCTGCTACAGAAAAAGCAGGCTTGGACAAATCAATCCAAGTATTGAAGGACAACATTAAGTTAGCTAAACTATAA", "aa": "MIGGLNQIKMKASLNKLVMKSLKVALIGSGAVGTSFLYAAMSRGLASEYMVIDINEKSQVGNVFDLQDAVPSSPQYSKVIAGDYKQLKDYDFIFIGAGRPQKQGGETRLQLLEGNVEIMKNIAKAVKESGFKGITLIASNPVDIMAYTYLKVTGFEPNKVIGSGTLLDSARLKFAIAEKYGMSSRDVQAYVLGEHGDSSVSIISSAKIAGLPLKHFSKASDIEKEFAEIDHFIRRRAYEIIERKGATFYGIGEATAEVAELILRDTKEVRVVASLINGQYGAKDVMFGTPCVLGRNGVEKILEIELSATEKAGLDKSIQVLKDNIKLAKL", "aa_hexdigest": "853c4494060764869b93776d963abaf6", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD6490", "uniref100_id": "UniRef100_P78007", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD6490", "UniRef:UniRef100_P78007" ] }, "ips": { "uniref100_id": "UniRef100_P78007", "uniref90_id": "UniRef90_P78007" }, "psc": { "uniref90_id": "UniRef90_P78007", "gene": "ldh", "product": "L-lactate dehydrogenase", "ec_ids": [ "1.1.1.27" ], "uniref50_id": "UniRef50_P78007", "cog_id": "COG0039", "cog_category": "C", "kegg_orthology_id": "K00016", "go_ids": [ "GO:0004459", "GO:0005737", "GO:0006089", "GO:0006096", "GO:0016020" ] }, "pscc": { "uniref50_id": "UniRef50_P78007", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P78007" ], "product": "L-lactate dehydrogenase" }, "genes": [ "ldh" ], "id": "APKECPOBEN_1411", "locus": "GCA002090215_01411" }, { "type": "cds", "sequence": "contig_1", "start": 799203, "stop": 799508, "strand": "-", "frame": 2, "gene": null, "product": "UPF0134 protein MPN_675", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013AFE3", "UniRef:UniRef100_P75117", "UniRef:UniRef50_P75076", "UniRef:UniRef90_P75117" ], "nt": "ATGGGCTTTTATGGCAATTTAAACCATATGGAAAAAGGCAAAAGCGGATACGTAACCCATAAACAATTGGACAAAAAACTAGAAGTTTTTAAACAAGAGCTTTTAGTTGAGTTTGATCAAAGATATGTTACTAAGGCAGAATTTAAAGAATTCCAAATAGAAGTTAGAGAAGGCTTTCGAATTCAGGGTGAAGCAATGAACGCCCGCATGGATCGCTTTGAAAGTCTTGTGCTTAAAAGCCTGGAATCAATTAACAATACCTTAATAGACTTTGGCAAACGTATAGACAAATTGGAATCTAAATAA", "aa": "MGFYGNLNHMEKGKSGYVTHKQLDKKLEVFKQELLVEFDQRYVTKAEFKEFQIEVREGFRIQGEAMNARMDRFESLVLKSLESINNTLIDFGKRIDKLESK", "aa_hexdigest": "71ea43af843ad1e29008d9c7697ae0cf", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013AFE3", "uniref100_id": "UniRef100_P75117", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013AFE3", "UniRef:UniRef100_P75117" ] }, "ips": { "uniref100_id": "UniRef100_P75117", "uniref90_id": "UniRef90_P75117" }, "psc": { "uniref90_id": "UniRef90_P75117", "product": "UPF0134 protein MPN_675", "uniref50_id": "UniRef50_P75076" }, "pscc": { "uniref50_id": "UniRef50_P75076", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75076" ], "product": "UPF0134 protein MPN_038" }, "genes": [], "id": "APKECPOBEN_1412", "locus": "GCA002090215_01412" }, { "type": "cds", "sequence": "contig_1", "start": 799862, "stop": 799999, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W5S1", "UniRef:UniRef90_A0AB38W5S1" ], "nt": "ATGGTTGATCGAAGGTTCGCAAACATTGAAGCGGAGTTGAGAGACTTCAAAGTAGAAGTTAGAGAAACTTTTAAAGTTGTGCTTCAGAGCCTTCAAGCCATCAACGAACGTTTAGATAGGTTTGAATACAAGAAATAA", "aa": "MVDRRFANIEAELRDFKVEVRETFKVVLQSLQAINERLDRFEYKK", "aa_hexdigest": "098ca20f459a5a429f789fe78fbc3d7c", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0AB38W5S1", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.978, "score": 82.4, "evalue": 4.27e-20, "valid": true, "uniref50_id": "UniRef50_A0AB38W5S1" }, "pscc": { "uniref50_id": "UniRef50_A0AB38W5S1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W5S1" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5531.3084, "isoelectric_point": 8.1404390335083 }, "id": "APKECPOBEN_1413", "locus": "GCA002090215_01413" }, { "type": "cds", "sequence": "contig_1", "start": 800776, "stop": 800889, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAAACAAGCACACTAACCTCAGTGAAATCAGTATGTTGGTAAAGCGTAGTAATGAACTGTTCTTTGAAAATGCACTGTACAAGTGGGCTAACGTTTTCTACCGCCTTTAA", "aa": "MQNKHTNLSEISMLVKRSNELFFENALYKWANVFYRL", "aa_hexdigest": "8b6d8dbb7a728f17c886c7e3bc5f6e96", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4536.196000000001, "isoelectric_point": 9.397636985778806 }, "id": "APKECPOBEN_1414", "locus": "GCA002090215_01414" }, { "type": "cds", "sequence": "contig_1", "start": 800923, "stop": 802053, "strand": "-", "frame": 1, "gene": "ydhJ", "product": "putative MG461-like protein", "db_xrefs": [ "COG:COG1078", "COG:R", "GO:0006203", "GO:0008832", "SO:0001217", "UniRef:UniRef50_P47699", "UniRef:UniRef90_P75115" ], "nt": "ATGCAACAAATCTTTTTTAAAGATCCCATTTTAGGGGAAGTGCTGTTTGACCAACAAACTAAGTGGATGTATGAGTTGGTGGAAACGGAAGCGTTTCGCCGTTTACGTAACATTAAGCAGCTAGGAATTAACTTTCATTTTTATCCGGGGGGCGTGCACACGCGTTATTCCCACTCCTTGGGGGTTTATGAACTGTTAAGGCGGATCTTAAACACCCCCGCGTTTGCTCCGATTGATGAAAACAAAAAGCAAACGGTGTTAGTAGCGGGATTACTGCACGACATTGGTCATGCACCGCATTCCCATGCCTTTGAGATTTACTTTGCCAAAGCCCCTAACTTTAAGAAGGAGCTGTTTATCCACGAAGAAGTTACAACCTTATTTGTCAACAGTGAACCGATTAAATCAATCTTAAAGGCGAACCAGATTGATCCTAAACTAGTAGCTGCTTTAATTGATGAAAACAAGGAGTTAAAGCCATCCAACTACTGGATGCGCCAGTTAATTTCATCTGACTTGGATGCTGACCGGATGGATTACCTGTTGCGCGATTCCTACTTTACGGGTACCAGTCATTCCTTGATAGATTACCAAACGATTATTAAGGAGATGGACTGTGTCAAGGTCAAGGGGATTTACGAAATCTTCTTTAAGGATAAGTGCTTGCCCTTAATTGAAAACTTCTTAATTACCCGGCACCACATGTACCAATCAATCTACAGCGATGGCAGGAGTATTTCCACCGAGTTAAACCTTTGGTTTGTCTTTCAACGAATTAAGGACTTAGTGGACAAAAACCAGTTTGACTTCAACGGTTATAAAACCTTAGAACAGGTGTGCTTACCACTACTCAAGAACGAACACTTTGACAAAAAGATGCTGCCAGCGTTTGTTAAGTTAGATGACTATGTGTTCCAATCGTTCTTTGTTAACTTGTACCAAACCACCAAGGACAAGATCTTAAAGAAGTTGTTGGACAGTTATTTAAACAGTTTGAAGTTCGAAATTAAGTTTTACGAAACCAAGGAACAGAGGGACTTAGACTTTGAAAAACAGGCGAGCAAGTACAAGGATGCTAAGTACTTTATTACGAAGTTTAACAATCAGTTTAAGGGCTTTTATGAGGGTTGA", "aa": "MQQIFFKDPILGEVLFDQQTKWMYELVETEAFRRLRNIKQLGINFHFYPGGVHTRYSHSLGVYELLRRILNTPAFAPIDENKKQTVLVAGLLHDIGHAPHSHAFEIYFAKAPNFKKELFIHEEVTTLFVNSEPIKSILKANQIDPKLVAALIDENKELKPSNYWMRQLISSDLDADRMDYLLRDSYFTGTSHSLIDYQTIIKEMDCVKVKGIYEIFFKDKCLPLIENFLITRHHMYQSIYSDGRSISTELNLWFVFQRIKDLVDKNQFDFNGYKTLEQVCLPLLKNEHFDKKMLPAFVKLDDYVFQSFFVNLYQTTKDKILKKLLDSYLNSLKFEIKFYETKEQRDLDFEKQASKYKDAKYFITKFNNQFKGFYEG", "aa_hexdigest": "41b5d44cf3184cccf5c2ffedb090e24b", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75115", "query_cov": 1.0, "subject_cov": 0.8847058823529412, "identity": 1.0, "score": 761.0, "evalue": 1.88e-277, "valid": true, "gene": "YdhJ", "product": "Uncharacterized protein MG461 homolog", "uniref50_id": "UniRef50_P47699", "cog_id": "COG1078", "cog_category": "R", "go_ids": [ "GO:0008832", "GO:0006203" ] }, "pscc": { "uniref50_id": "UniRef50_P47699", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47699" ], "product": "Uncharacterized protein MG461" }, "genes": [ "ydhJ" ], "id": "APKECPOBEN_1415", "locus": "GCA002090215_01415" }, { "type": "cds", "sequence": "contig_1", "start": 802034, "stop": 802495, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF19269.4" ], "nt": "TTGAGTGATAATGCCTACTTTAACTTTATTGATAACTACTTAACGATTGACTTTGATTACCTCAAGAACAAGCGCAAAGAAGTCAGTCTGTTGTTCAAAAACCAACTAGCGTTTGGCATTGAAATTAACCAACTGATTAAGGAAACCTTTGCCCCCAAGTTAGGGGTGCAGCATTTAAGTGTTAAGCACCGTGAACTCTTCAAAGAGCTCCAATCAGCCCTACAACAACTGAGTGAACAACTCCAAGCCCTACCTGATTGGACGAAGGACAATGTCAAAAGTACCTTAACCCAAATTGGTGAACAGTTTAATCTGAAGGGCAAAAAGCTGTTTATGCCACTACGGTTGATCTTTACCAACAAAGAGCATGGTCCTGATTTGGCGGGCATCATGGTGCTGCACGGTAAGACTCAGGTATTAGCGCTGCTTCAAGAATTTATTCATGCAACAAATCTTTTTTAA", "aa": "MSDNAYFNFIDNYLTIDFDYLKNKRKEVSLLFKNQLAFGIEINQLIKETFAPKLGVQHLSVKHRELFKELQSALQQLSEQLQALPDWTKDNVKSTLTQIGEQFNLKGKKLFMPLRLIFTNKEHGPDLAGIMVLHGKTQVLALLQEFIHATNLF", "aa_hexdigest": "93e9bd6b2bdb07f6f2f7d800aa4a175b", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF19269.4", "name": "Anticodon_2", "length": 127, "aa_cov": 0.8104575163398693, "hmm_cov": 1.0, "evalue": 5.615317871748724e-20, "score": 67.65188598632812, "start": 23, "stop": 146 } ], "seq_stats": { "molecular_weight": 17706.353199999987, "isoelectric_point": 8.780029869079591 }, "id": "APKECPOBEN_1416", "locus": "GCA002090215_01416" }, { "type": "cds", "sequence": "contig_1", "start": 802631, "stop": 802930, "strand": "-", "frame": 3, "gene": null, "product": "glutamate--tRNA ligase family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002849521E" ], "nt": "TTGAAGGCCAATGGCATTGCCACGTATAACTTTGCCGTAGTCATTGATGACCATGACATGGAAATTACCGATGTGTTGCGGGGGGCGGAACACATTTCCAACACCGCTTACCAACTAGCAATTAACCAAGCCTTAGGCTATCAAAGGATTCCCCGCTTTGGTCATTTATCGGTGATAGTCGACAAGAGTGGCAAGAAACTGTCTAAGCGTGATACCAAAACGATCCAGTTCATTGAACAGTTCAAACAAGAGGGTTACCTACCAGAAGCGGTGGTAAACTTCTTGGCCTTACTGGGTTGA", "aa": "MKANGIATYNFAVVIDDHDMEITDVLRGAEHISNTAYQLAINQALGYQRIPRFGHLSVIVDKSGKKLSKRDTKTIQFIEQFKQEGYLPEAVVNFLALLG", "aa_hexdigest": "55a97675094a3aea2b1a3abc8eeb52f4", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0037046112", "query_cov": 0.9292929292929293, "subject_cov": 0.8288288288288288, "identity": 0.598, "score": 114.0, "evalue": 3.63e-31, "valid": false, "product": "glutamate--tRNA ligase family protein", "uniref50_id": "UniRef50_UPI002849521E" }, "pscc": { "uniref50_id": "UniRef50_UPI002849521E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002849521E" ], "product": "glutamate--tRNA ligase family protein" }, "genes": [], "id": "APKECPOBEN_1417", "locus": "GCA002090215_01417" }, { "type": "cds", "sequence": "contig_1", "start": 802991, "stop": 803488, "strand": "-", "frame": 3, "gene": null, "product": "Glutamate--tRNA ligase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A654IDE7" ], "nt": "ATGGAAAAGATTAGAACCCGTTATGCCCCTTCCCCTACCGGTTACCTTCATGTTGGGGGTGCGCGTACTGCTATCTTTAACTTCTTGTTAGCAAAACACTTTAACGGCGAGTTTATTATCCGGATTGAGGACACTGACACTGAGCGCAATGTGGAAGGGGGAATTGAATCACAACTAGAAAACTTGCGCTGGTTAGGAATTATTCCCGATGAATCAATTTACAACCCGGGTAATTATGGGCCTTACATCCAAAGTCAAAAGTTGGCTACCTACAAAAAACTAGCTTATGAACTTGTGGGCAAGGGTTTGGCCTACCGTTGTTTCTGTACTAAAGAAAAGTTAGAACACGAACGCCAATTGGCTTTAGAACACCACCAAACCCCGAAGTATTTGGGAACGTGTCGCAACTTACACTCCAAGCACATTCAAACAAACTTAGACAACCAAGTACCCTTCACGATTCGCTTAAAGATTAACCAGGACGCTGAGTTTGCCTGA", "aa": "MEKIRTRYAPSPTGYLHVGGARTAIFNFLLAKHFNGEFIIRIEDTDTERNVEGGIESQLENLRWLGIIPDESIYNPGNYGPYIQSQKLATYKKLAYELVGKGLAYRCFCTKEKLEHERQLALEHHQTPKYLGTCRNLHSKHIQTNLDNQVPFTIRLKINQDAEFA", "aa_hexdigest": "625e0b899028ac41140e30eaeea8ae4a", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A654IDE7", "query_cov": 0.8666666666666667, "subject_cov": 0.9727891156462585, "identity": 0.58, "score": 162.0, "evalue": 2.71e-48, "valid": false, "product": "Glutamate--tRNA ligase", "uniref50_id": "UniRef50_A0A654IDE7" }, "pscc": { "uniref50_id": "UniRef50_A0A654IDE7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A654IDE7" ], "product": "Glutamate--tRNA ligase" }, "genes": [], "id": "APKECPOBEN_1418", "locus": "GCA002090215_01418" }, { "type": "cds", "sequence": "contig_1", "start": 803475, "stop": 804266, "strand": "-", "frame": 2, "gene": "rsmA", "product": "16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase RsmA", "db_xrefs": [ "COG:COG0030", "COG:J", "EC:2.1.1.182", "GO:0000179", "GO:0003723", "GO:0005829", "GO:0006364", "GO:0052908", "RefSeq:WP_010875036.1", "SO:0001217", "UniParc:UPI000012E0AF", "UniRef:UniRef100_P75113", "UniRef:UniRef50_P47701", "UniRef:UniRef90_P75113" ], "nt": "GTGAATAGTTTTTATCCTTCACGCAAATTAGGGCAAAACTTTACGGTTGACCAGAGTGTAATTGCCAAAACCTGTCGGTTAATTAAGAGTCTCAACCCAACGGCCTTAATTGAAGTGGGGCCGGGTAAGGGAGCGTTAACCAAGGCTTTACTCAAGTTACAACTACCATACCATGGCATAGAGTTGGATAAACGCTTAGCGGAATACCTCTTGGTCAACGAAATCTTAACTGAAGAACAACTAACCATTGGTGATGCCTTAAAACAAAACTTGGATCAGTATTTTCCTGACACTATACCTTTGCTTTGTGGCAACATTCCCTACAGTATTAGCTCCCCTTTGATTGCTAACTTTTTGGCTTCCAAACTACAACAATTTGTGCTGGTGTGCCAGTGGGAGTTTGGTCAAAGGTTAGTAGCACCGGTAAATTCGCCTAACTATAGTGCCTTTGGTGTGTTTTGCCAATACCATTTGCAAATTAAATCAGTTTTTAAAATTGATAAGGTTGCTTTTAAGCCCAAGCCCCAAGTGGATTCGGTGTTAATGTTGTTAAAAAAGAAACCCCAAGTAGCCTATGAAGCTCATTTTGGCCGGTTCTTAAAACAGTGTTTTCACCAGCGCCGCAAGCTGTTAGTCAATAACCTCAAGCAGCTTCTCCCACCCACCTTGTTAACTAATGTTTTACAACAACAGGACTTGGCGGCAACGGTTAGAGCCCAAGAGTTAACCCCGACCCAGTTGTTTCGTTTGTATCTTAGCCTTAAACCACATTTAAGCGATGGAAAAGATTAG", "aa": "MNSFYPSRKLGQNFTVDQSVIAKTCRLIKSLNPTALIEVGPGKGALTKALLKLQLPYHGIELDKRLAEYLLVNEILTEEQLTIGDALKQNLDQYFPDTIPLLCGNIPYSISSPLIANFLASKLQQFVLVCQWEFGQRLVAPVNSPNYSAFGVFCQYHLQIKSVFKIDKVAFKPKPQVDSVLMLLKKKPQVAYEAHFGRFLKQCFHQRRKLLVNNLKQLLPPTLLTNVLQQQDLAATVRAQELTPTQLFRLYLSLKPHLSDGKD", "aa_hexdigest": "3bd4dccd0ca3a6ffdda6131a30d13600", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000012E0AF", "ncbi_nrp_id": "WP_010875036.1", "uniref100_id": "UniRef100_P75113", "db_xrefs": [ "SO:0001217", "UniParc:UPI000012E0AF", "RefSeq:WP_010875036.1", "UniRef:UniRef100_P75113" ] }, "ips": { "uniref100_id": "UniRef100_P75113", "uniref90_id": "UniRef90_P75113" }, "psc": { "uniref90_id": "UniRef90_P75113", "gene": "rsmA", "product": "16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase RsmA", "ec_ids": [ "2.1.1.182" ], "uniref50_id": "UniRef50_P47701", "cog_id": "COG0030", "cog_category": "J", "go_ids": [ "GO:0000179", "GO:0003723", "GO:0005829", "GO:0006364", "GO:0052908" ] }, "pscc": { "uniref50_id": "UniRef50_P47701", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47701" ], "product": "Ribosomal RNA small subunit methyltransferase A" }, "genes": [ "rsmA" ], "id": "APKECPOBEN_1419", "locus": "GCA002090215_01419" }, { "type": "cds", "sequence": "contig_1", "start": 804259, "stop": 804558, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCAATTTATCTCGCAAAAGTTGCCCCAAATTTGGGCTAGCAAACGCAATGAGAATGCTAAAGCGCATTCGCAAAAGAGCATTGAACAGCTCAATAAAACAAAGCGAATGCAGTTAATTTTCTACTTTGTGTTTGCTGTTATTACGGCTTTTAGTGCCGCTGGTGTGGGTGTGTACTGGTTCTTAAATGCCTTGTTTACCTTACTCCAGTCTTATCTAACCCACCTCTTTATTGTTAAAAGGCGTACCAGAAGACGGTTAACGTATTCCAAATTAGAACAAATGTTAGAACGTGAATAG", "aa": "MQFISQKLPQIWASKRNENAKAHSQKSIEQLNKTKRMQLIFYFVFAVITAFSAAGVGVYWFLNALFTLLQSYLTHLFIVKRRTRRRLTYSKLEQMLERE", "aa_hexdigest": "58fb5a875f8968c7bcd6e65d0c32bcd5", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11818.760199999988, "isoelectric_point": 10.620344352722167 }, "id": "APKECPOBEN_1420", "locus": "GCA002090215_01420" }, { "type": "cds", "sequence": "contig_1", "start": 804592, "stop": 805020, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTACGCTACCAGGGTTCCTCTAGGTAGCGGTCCGGAATTGGGCTTCAACATGATCTTGTCTTTGATTGTGTTGTTGTTCCTAGTACGGTTAATTACCATAGTGATTACCCTCAACTCCACGCTGGCGTTAGAAAAAATGAACGAGGTCCAGGGCAAATTAGCAGAAATTAATGCCAAGTACAAAGGGGCGTTGGACTTACAGAGCAAACGCAACCGCCAGATGGAGATCATGTCCTTGTACAAGAAGCACAACATTAAGTCTTCTGCTTCCTTTGTTCAGGTCTTTGTTACCTTACCCATCTTCCTCATTATTTACCGGATTGTCACGACTTTAAGACCAATTAAAGCGATTATCCTGTTTAACTTCTGGGACTTATCTAAGGTACCGTTAACGGAAATCTTCTCGAACTTTACCAGTACCGGGTGA", "aa": "MYATRVPLGSGPELGFNMILSLIVLLFLVRLITIVITLNSTLALEKMNEVQGKLAEINAKYKGALDLQSKRNRQMEIMSLYKKHNIKSSASFVQVFVTLPIFLIIYRIVTTLRPIKAIILFNFWDLSKVPLTEIFSNFTSTG", "aa_hexdigest": "2f220ddcb11b846e54f198a5e5a794ec", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16096.09469999999, "isoelectric_point": 9.992873573303225 }, "id": "APKECPOBEN_1421", "locus": "GCA002090215_01421" }, { "type": "cds", "sequence": "contig_1", "start": 805397, "stop": 805546, "strand": "-", "frame": 3, "gene": "rnpA", "product": "ribonuclease P protein component", "db_xrefs": [ "COG:COG0594", "COG:J", "EC:3.1.26.5", "GO:0000049", "GO:0001682", "GO:0004526", "GO:0008033", "GO:0030677", "GO:0042781", "SO:0001217", "UniParc:UPI0001DD64AE", "UniRef:UniRef100_P75111", "UniRef:UniRef50_P47703", "UniRef:UniRef90_P75111" ], "nt": "ATGCGGGAACAGTTTTGCAATTTAAACGCTGTTGATGTTTTGGTTATTATTAATCAAGGCTTCCTTGAATTAACATTTAAAGAAAAGCAAACGATCTTTTTAAACCTTTGCCAAAAGCTCCAAGAACTCGATGCCCCTAAACCTAAATAA", "aa": "MREQFCNLNAVDVLVIINQGFLELTFKEKQTIFLNLCQKLQELDAPKPK", "aa_hexdigest": "f7b14340e2ac70ad9eba8d9433301b42", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD64AE", "uniref100_id": "UniRef100_P75111", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD64AE", "UniRef:UniRef100_P75111" ] }, "ips": { "uniref100_id": "UniRef100_P75111", "uniref90_id": "UniRef90_P75111" }, "psc": { "uniref90_id": "UniRef90_P75111", "gene": "rnpA", "product": "ribonuclease P protein component", "ec_ids": [ "3.1.26.5" ], "uniref50_id": "UniRef50_P47703", "cog_id": "COG0594", "cog_category": "J", "go_ids": [ "GO:0000049", "GO:0001682", "GO:0004526", "GO:0008033", "GO:0030677", "GO:0042781" ] }, "pscc": { "uniref50_id": "UniRef50_P47703", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47703" ], "product": "Ribonuclease P protein component" }, "genes": [ "rnpA" ], "id": "APKECPOBEN_1422", "locus": "GCA002090215_01422" }, { "type": "cds", "sequence": "contig_1", "start": 805628, "stop": 805753, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAGCGCTAAGACCAAGCACCACCTCCGTGATCGTAAAGTATTTGCTGCCCTTTTAAGGAGTAAAAGACGCTTCTTCTCGACCTTTTTGATGGCCTACTTTATGCCGAACCGCGTCCGCACTTGA", "aa": "MSAKTKHHLRDRKVFAALLRSKRRFFSTFLMAYFMPNRVRT", "aa_hexdigest": "00ce52d5ef20012e906f3ddf8b70315d", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5016.966, "isoelectric_point": 11.999967765808105 }, "id": "APKECPOBEN_1423", "locus": "GCA002090215_01423" }, { "type": "cds", "sequence": "contig_1", "start": 805743, "stop": 805889, "strand": "-", "frame": 2, "gene": "rpmH", "product": "50S ribosomal protein L34", "db_xrefs": [ "COG:COG0230", "COG:J", "GO:0000315", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625", "GO:1990904", "KEGG:K02914", "RefSeq:WP_010875039.1", "SO:0001217", "UniParc:UPI0000133FBC", "UniRef:UniRef100_P78006", "UniRef:UniRef50_Q7X5L3", "UniRef:UniRef90_P78006" ], "nt": "ATGAAACGTACATATCAACCAAGTAAATTAAAGCGAGCAAAAACCCATGGCTTTTTAGCTCGCATGGCCACCGCTTCTGGTCGTAAAGTATTGAAGTTAAGAAGAAAAAAACAACGCGCCCAACTCACCGTATCCAGTGAGCGCTAA", "aa": "MKRTYQPSKLKRAKTHGFLARMATASGRKVLKLRRKKQRAQLTVSSER", "aa_hexdigest": "077659cf990bc319d68efbe827197fe7", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000133FBC", "ncbi_nrp_id": "WP_010875039.1", "uniref100_id": "UniRef100_P78006", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000133FBC", "RefSeq:WP_010875039.1", "UniRef:UniRef100_P78006" ] }, "ips": { "uniref100_id": "UniRef100_P78006", "uniref90_id": "UniRef90_P78006" }, "psc": { "uniref90_id": "UniRef90_P78006", "gene": "rpmH", "product": "50S ribosomal protein L34", "uniref50_id": "UniRef50_Q7X5L3", "cog_id": "COG0230", "cog_category": "J", "kegg_orthology_id": "K02914", "go_ids": [ "GO:0000315", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_Q7X5L3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q7X5L3" ], "product": "Large ribosomal subunit protein bL34" }, "genes": [ "rpmH" ], "id": "APKECPOBEN_1424", "locus": "GCA002090215_01424" }, { "type": "cds", "sequence": "contig_1", "start": 805912, "stop": 806496, "strand": "-", "frame": 1, "gene": "lolD", "product": "Putative ABC transporter ATP-binding MG467-like protein", "db_xrefs": [ "COG:COG1136", "COG:M", "GO:0005524", "GO:0016887", "SO:0001217", "UniRef:UniRef50_P75110", "UniRef:UniRef90_P75110" ], "nt": "GTGATTATTTTAGGTCCATCAGGTTCGGGGAAAACTACCCTTTTAAACCTAATAGGCGGTTATGACTCAATTTCTTTGGGCAGCTGTGTGGTGGCCAATTGTCCCTTGGAAAAATGTACCACCGAACAGCTCTTAACTTACCGCAAGTTAAACCTGGGTTATGTGTACCAACGCTATAACCTCATAGAACTCTTAAGTGCGTATGACAACATTGCCATCTCGCAAAACCTAATTCCTAAAGAACAACGCCACTTAGACATCGAGGAGTTAGCGGCTAAACTCGACATTAAGGAAATCTTGTACAAGTTTCCGTACGAGATGTCGGGGGGTCAAAAGCAACGGGTGGCGATTGCCCGTGCCATTATTAAAGAACCGCGCTTGTTGCTCTGTGACGAACCTACGGGGGCTTTAGACAGTAATTCAGCCGAAAACATTATTGCCTTATTACAAGCAATCAATAAGGAATATAAGCAAACTATTCTAATGGTGACCCACGATGAAACGTTAACGCGGATCGCTAACAGGATCATTAAGATCTCCGATGGTAAGATAGTGTCTAACGAATTGGTGCGTCCTTTATCTTAG", "aa": "MIILGPSGSGKTTLLNLIGGYDSISLGSCVVANCPLEKCTTEQLLTYRKLNLGYVYQRYNLIELLSAYDNIAISQNLIPKEQRHLDIEELAAKLDIKEILYKFPYEMSGGQKQRVAIARAIIKEPRLLLCDEPTGALDSNSAENIIALLQAINKEYKQTILMVTHDETLTRIANRIIKISDGKIVSNELVRPLS", "aa_hexdigest": "99686f010059a8b4df434a739a78c711", "start_type": "GTG", "rbs_motif": null, "genes": [ "lolD" ], "truncated": "3-prime", "pseudogene": { "start": 805912, "stop": 806685, "observations": { "mutation": [ 806580, 806661 ], "stop": [ 806580, 806661 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75110", "query_cov": 1.0, "subject_cov": 0.5722713864306784, "identity": 0.995, "score": 377.0, "evalue": 3.59e-130, "gene_start": 1, "gene_end": 194, "reference_start": 146, "reference_end": 339, "reference_sequence": "MKDTEKKTEKELVWDECVDILENVKANSFKATLEYYLASDKKTKKAKPAKVKKVKEPKAKAVKPEQVKPTKTTKAPKPKKPKKQGGLISQWKEKLEAPYRTPYVEQKQGMVISIDKMWKHVHGEDSKEQIAILSDVSLEIGYGEIVIILGPSGSGKTTLLNLIGGYDSISLGSCVVANCPLEKCTTEQLLTYRKLNLGYVYQRYNLIELLSAYDNIAISQNLIPKEQRHLDIEELAAKLDIKEILYKFPYEMSGGQKQRVAIARAIIKEPRLLLCDEPTGALDSNSAENIIALLQAINKEYKQTILMVTHDETLTRIANRIIKISDGKIVSNELVRPLS" }, "paralog": false, "identity": 0.9922178988326849, "score": 493.0, "evalue": 3.9e-178, "description": "Internal stop codon at 806580, 806661. Nonsense mutation around 806580, 806661.", "psc": { "uniref90_id": "UniRef90_P75110", "gene": "LolD", "product": "Putative ABC transporter ATP-binding protein MG467 homolog", "uniref50_id": "UniRef50_P75110", "cog_id": "COG1136", "cog_category": "M", "go_ids": [ "GO:0005524", "GO:0016887" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75110", "UniRef:UniRef50_P75110", "COG:COG1136", "COG:M", "GO:0005524", "GO:0016887" ] }, "pscc": { "uniref50_id": "UniRef50_P75110", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75110" ], "product": "Putative ABC transporter ATP-binding protein MG467 homolog" } }, "id": "APKECPOBEN_1425", "locus": "GCA002090215_01425" }, { "type": "cds", "sequence": "contig_1", "start": 806659, "stop": 806931, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGATACAGAAAAGAAAACCGAGAAAGAACTTGTCTGGGACGAGTGTGTTGATATATTAGAAAACGTTAAGGCCAACAGCTTTAAGGCCACCTTGGAGTATTATTTAGCATCAGACAAGAAAACAAAGAAGGCCAAACCCGCTAAGGTGAAAAAAGTTAAGGAACCGAAGGCGAAAGCAGTTAAACCGGAACAAGTTAAACCCACGAAGACCACCAAGGCACCTAAACCCAAAAAACCGAAAAAGCAGGGTGGGTTGATCTCCCAGTGA", "aa": "MKDTEKKTEKELVWDECVDILENVKANSFKATLEYYLASDKKTKKAKPAKVKKVKEPKAKAVKPEQVKPTKTTKAPKPKKPKKQGGLISQ", "aa_hexdigest": "72b5dc402f4736b3e5f9d631b6f73eef", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10167.930099999992, "isoelectric_point": 9.882503700256347 }, "id": "APKECPOBEN_1426", "locus": "GCA002090215_01426" }, { "type": "cds", "sequence": "contig_1", "start": 806972, "stop": 807406, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTGCCAGCTCTCAAATTAGATGCCGCTGGTGCTAGTGCTCAAATTATCAGGAATAATGCTGAGGTGTTGTTTAATACCGTGAACCAAGTTGATGGGTTCTTGTTGGGCACCATTATTCCCTTTATCTTTATTACCTGTGTGGTCTTGGGGATCTCGATGTTAGAGGAAATGAAACGGATCTTTATTTCCTTAAAATCGATTGGTTACAAAGATAGTCAAAACTTAGTTAGTCTCTTGTGCTTCTTTATTCCCGCTTTTGTACTTTCGTTATTAATCTCGATTGCTATCTTAGCTGGGTTACTAGTAGGAGTACAAGCCCTAGTCTTTGGGGTTGCCCAGGTCTTTTTAACCAACGTGTTTGAATTCTTACCTTACATGGTGGGGATAGTCCTGTTTGGTGCTACCATCTTTGTAATAGGTTCTTACTTCTGA", "aa": "MVPALKLDAAGASAQIIRNNAEVLFNTVNQVDGFLLGTIIPFIFITCVVLGISMLEEMKRIFISLKSIGYKDSQNLVSLLCFFIPAFVLSLLISIAILAGLLVGVQALVFGVAQVFLTNVFEFLPYMVGIVLFGATIFVIGSYF", "aa_hexdigest": "afc3bf22dd50d791de4defe46e49f6af", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15601.642999999982, "isoelectric_point": 5.095865821838378 }, "id": "APKECPOBEN_1427", "locus": "GCA002090215_01427" }, { "type": "cds", "sequence": "contig_1", "start": 807461, "stop": 809176, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACTCCCTTGGGTAAATTCCGGGTGTCCTTGATTATTTCCCGGTTATCGCGGTTATTTACCTATGTGGGGTTAAGTTCGATTGCCCTGCTTTTAATTGGGATTGCCGGTACCATCCCGCAAAAGTTTGGGGCAGCGCAGTCCAACACCGTTTTAAACCGCAACTTTAACTATAGGTTGAACTTGCAAACCCCCACAGAACAATCCGGCTGGTATGCAATCCAACCCTACTCCCGGTTTGGTCAAACTGATGATAGTTTGGGGATTAAGGCCTTGTATAAAGATAAGGGTGATCAAATTCAGCAACAGCAACAGCAGCAACAACAGCAAGGCAACGACAAAGAACACCCATACAACCTCAAAGAGTTAAAGATTAGTGATAGGGGCGGAAACCCAATCAAGCATAATGGTAAGGAAATCGAACTCGGTAACCTCCTTCTCCCAAGTTTTGGTGGTGCCCAACAATTAAACACTGACGAAAACTTCTTCCGTCATGCTTCGTTGTCGAAGTGGCTCATTGACTTCCCAATTCGCGTTGGTGGTGCCAACATTAACCCGTGGGAAATTGTCGAGAAGTCCATTCCTAAACAAATTACCCAATTGTTATCGGCTTCCAGTGACCAGTTTTTAATTGCGGTTCTTACTGATGACTACTTTAATAACCTCAACAATAATGGTTTCTTAACCCGCAACCCTCGGACTAACTTTATTCAGTTGGATGCCGCTAGGGTATTAACCCAGATTAACGTCTTCAATCCTGGTGGAGTTAAATTTAACGAACAATTCCTTAAATTCCTTACTAAGGTCTATGGTGATCCGGAGTTGTCCTACCAAGACTCGAAGTTAACGTACGGGATTGTCCCGGTTGATCCACAAATAGAGGAGACATACACCTATGTTCAAGGACCATTTGGTTTTAAGGAAACCGAGTTGAATCCTGATTCCCCTTACACCTTAACGGGAATTAGTCCCGATTCCAAGTTTGTCAACCTCACTGACAGCGGTGGTAACTCCTTGAGGAGCTTAATTAGCTCTGACAGTGAGATGAATGTCATTGTCAACGCTGGCTTTCAGTACGCTAATAACACCAAGATCGGGGACTTCATCTTTATTCAACCCAAGAACACTGCGACCCGCTATTCAGAGAAGTTCTTAAAGAGCCCACCTAAAACACCAACCGTGAAGTTCCGGGTTGTCGGGGTATCTACCGATGCCTTTGGCCAAGAGTTGTATATTAACCAAAACATTGCCAACCGCTTGTTAAAGCTCAATGGCTTTGATGGCCGCGGGGTCATTAAGGATGTGGTGAAGGACGGCCAATCCACCGATGATAGCGGTGGTACTTCGTCTGGCGGTGGCTCTTGTGGGGGTGGAAGTACCTCTTCTACCACCAAAGACAAGTACAAGATTGAGTACGTCAAGCCAACTGGTTATGTTCCCTTTAACGGGGTCTTCTCCAAGGAACTCAACCCTTCTTTGGTAAGTAAAGCGCTAGTACTTAACTCCAACATTGGTGTCTGGGGTAACTTTACGGATTTTGGTAATAACTTTACTAACCTCGTTAAGGGTAAGGAAAACAAGATTATTACCTCAATCTTACCAAGTGATCCGGATATCTTAAAGCAGTTGGCTAAGGAAAAGGGTGAGAATGGTGTGGATAGCATGACCTATGAAAACCTCAGAAAAAAGGTTATCGAAAAATATACGAGCGAATGA", "aa": "MTPLGKFRVSLIISRLSRLFTYVGLSSIALLLIGIAGTIPQKFGAAQSNTVLNRNFNYRLNLQTPTEQSGWYAIQPYSRFGQTDDSLGIKALYKDKGDQIQQQQQQQQQQGNDKEHPYNLKELKISDRGGNPIKHNGKEIELGNLLLPSFGGAQQLNTDENFFRHASLSKWLIDFPIRVGGANINPWEIVEKSIPKQITQLLSASSDQFLIAVLTDDYFNNLNNNGFLTRNPRTNFIQLDAARVLTQINVFNPGGVKFNEQFLKFLTKVYGDPELSYQDSKLTYGIVPVDPQIEETYTYVQGPFGFKETELNPDSPYTLTGISPDSKFVNLTDSGGNSLRSLISSDSEMNVIVNAGFQYANNTKIGDFIFIQPKNTATRYSEKFLKSPPKTPTVKFRVVGVSTDAFGQELYINQNIANRLLKLNGFDGRGVIKDVVKDGQSTDDSGGTSSGGGSCGGGSTSSTTKDKYKIEYVKPTGYVPFNGVFSKELNPSLVSKALVLNSNIGVWGNFTDFGNNFTNLVKGKENKIITSILPSDPDILKQLAKEKGENGVDSMTYENLRKKVIEKYTSE", "aa_hexdigest": "b4487ab33f2c662e1b52f28546df8ee2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 63269.45210000015, "isoelectric_point": 8.779836463928223 }, "id": "APKECPOBEN_1428", "locus": "GCA002090215_01428" }, { "type": "cds", "sequence": "contig_1", "start": 809201, "stop": 809326, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCAAGAAGACCGAGAACTTGGTTTCATTACCCTGGTCAATCAGGACATTAACCGGTTTACTAAAGAGCTGTTTAAAGGCTATTCAGCTAATAAACTGGAAGATCACAAAGATCAACAGCATTGA", "aa": "MQEDRELGFITLVNQDINRFTKELFKGYSANKLEDHKDQQH", "aa_hexdigest": "d53d7337194de875612b233604a11f18", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4937.417500000001, "isoelectric_point": 5.473106193542479 }, "id": "APKECPOBEN_1429", "locus": "GCA002090215_01429" }, { "type": "cds", "sequence": "contig_1", "start": 809285, "stop": 809935, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02687.27" ], "nt": "TTGTCCACAAACTCACCGCTGCCCAACTCCCAGGATGAAGGCTTAATCTTTCTCAATGAGCAGGGATACCGCAGTGTCTTGTTTGCTGTGCCTGCTGCTAGTGAGGAGAACTACTACGCCTTTAAGTCGGATGACATTAAAGCGAAGTTCCCTGGCCAAGATCCGATCCAGGTGGTGGCCTCTAAGTTAAAGGGCTATCTCAACGTCCCTGACAGTGATCTCGCCTTTAACGTTAAGGACATCTCCAAGTTCCAGTACTTAACTACAGCCCGGAACTATTTCCCGGACTTAGTGCAGAACTATTTAGCCATTGCCAGTGTGGTCATTGCGGCCTTCTTATCCATCTTGGCCTTATACCTCACCATTCTCTTAATTAAGTCTTTTATTAAGAAGAACCAAACGGAGTTCTCAATTATCCGCGCTGGTGGGTTCTCCACAGCCAAGTTTATTGCCGGCATGAGTGTGTTTGCCGGCATAGTAGCCTTGGCCTCATCCTTCTTTGGGGTCTTATTTGCCTTCCTTTTAGAAAGACAAGTTAAGGGGATTATCTCCAGGTATTGGTTTATAGCCCTACCGGCCAACAGCTTTAACTGGATTTCCTTCTTTGGATCAATGCTGTTGATCTTTGTGATCTTCCAGTTTATTAGCTGA", "aa": "MSTNSPLPNSQDEGLIFLNEQGYRSVLFAVPAASEENYYAFKSDDIKAKFPGQDPIQVVASKLKGYLNVPDSDLAFNVKDISKFQYLTTARNYFPDLVQNYLAIASVVIAAFLSILALYLTILLIKSFIKKNQTEFSIIRAGGFSTAKFIAGMSVFAGIVALASSFFGVLFAFLLERQVKGIISRYWFIALPANSFNWISFFGSMLLIFVIFQFIS", "aa_hexdigest": "658120ad7211e625058e72ed5c370a57", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02687.27", "name": "FtsX", "length": 109, "aa_cov": 0.5046296296296297, "hmm_cov": 0.981651376146789, "evalue": 5.5164286329301714e-12, "score": 41.29035568237305, "start": 108, "stop": 216 } ], "seq_stats": { "molecular_weight": 24064.715899999992, "isoelectric_point": 9.023333549499512 }, "id": "APKECPOBEN_1430", "locus": "GCA002090215_01430" }, { "type": "cds", "sequence": "contig_1", "start": 810071, "stop": 810916, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAAGAAGGTCACCGCGTTATTAGAAAAATACCGCGATGCCTTCTTAAAGGCGAAAGCCTTAAAGGAATTGGAGTTTTCCCGGCAAAACTTAGCGATTGCTACCAATGTTAGCTCCGAAGAATCCGCTTCCTTCTTGGTCTCCAATAAGGATTCCCAGAAGTACAACGACCTTTCTATTATTGAAGGGATTAACTTTAAGTCTTGGCTTGCCAAGGAAAAAAGCAATCCTTTAGACATGGTATATGGGGGCAAGAGTAACAGTGAAGGCTTCTTGGAAAAGGTTGAGTATGAGTTTAAGCCTACTCAAACAGATGAAAAGAAAAAAGCAGCAGCCAAAACTACGCAAGGCACAACGGACTCTTTAACCCAGTTAGCAGATGCATCTTCTTCCTCCAGCTCCTCTTCAACTGGTGATACTAAGAGTACTTCTACCAAATTCCAAATCTACCCCAAACTAGCTAACATCTTAGCCCAAGCCCAACTCCCCGAGGCTTCCAGTATTCCTGATACCTTAACCAATGCCATTAAGCAGTGGTCTACATTAGATAAAAAAGGCTTTGAGGCTTTAGATGATACAGGTAAGAGTAAAGCCGCTAACAACTATGTTGCTTTATTGAGTTACTTTACCCCGGAGTTTCAAGATCCGAATGAACTGGTAGTAACTAACCGCCAAAAGTTAGATATCCCGATTATCTTTAAAAACGGGGTTAACCCGTTAACACTACCCACCGACCAACAGTCCTTGGTAGTCCAAACCCCGGAAGCCCACGGTGCGGTGGTATCCCAGCAATGGCTATTTAAACACGACAAGGAAGTATTACCTTTAGAAGGGGAATACTCCTGA", "aa": "MKKVTALLEKYRDAFLKAKALKELEFSRQNLAIATNVSSEESASFLVSNKDSQKYNDLSIIEGINFKSWLAKEKSNPLDMVYGGKSNSEGFLEKVEYEFKPTQTDEKKKAAAKTTQGTTDSLTQLADASSSSSSSSTGDTKSTSTKFQIYPKLANILAQAQLPEASSIPDTLTNAIKQWSTLDKKGFEALDDTGKSKAANNYVALLSYFTPEFQDPNELVVTNRQKLDIPIIFKNGVNPLTLPTDQQSLVVQTPEAHGAVVSQQWLFKHDKEVLPLEGEYS", "aa_hexdigest": "11bda8aa8e40cb8798c5eea825e53613", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 31020.43390000002, "isoelectric_point": 5.6295270919799805 }, "id": "APKECPOBEN_1431", "locus": "GCA002090215_01431" }, { "type": "cds", "sequence": "contig_1", "start": 810941, "stop": 811162, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTGCACTTCGCTCTTCTGTTTCTTTCAAGCCGCAATTACAACAAACGAACGTTGCGTTGGCTCAGCAACAACAAGATAAGCAGGAAAGTAGTGCAGATGACGGTGTAAAAGATCCAACTTTTAGTGATGTCCAAACTGAATTCGACAAAATTGGCACCGAAAACCACACTCCGCAAAAGAATTTAAATAACGTTTATGCTGCCCTTTTACACCAATGA", "aa": "MIALRSSVSFKPQLQQTNVALAQQQQDKQESSADDGVKDPTFSDVQTEFDKIGTENHTPQKNLNNVYAALLHQ", "aa_hexdigest": "056c214fb13dbbed568e977ab32818e0", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8143.847, "isoelectric_point": 5.034650230407714 }, "id": "APKECPOBEN_1432", "locus": "GCA002090215_01432" }, { "type": "cds", "sequence": "contig_1", "start": 811409, "stop": 812581, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTTCGTTTTTTAAACAGATCTTTAAGTCACTCAAAAAGTTCTTCTTTTTACTTTTTGGGATTATTTTCGTCCTTTTCAGCATTATCTTCCTGGAAACGTCGATTCTTCAGTTAAGTAATAACCTTGTTAATACTTATACTGCCCTTGTTCAAAAAACGAATTCTTCTGACATAGTAGCACCGGCTATCTTTAAAGAAAGTAGTCCGGTGTATAAAACGGAACTTAAAGAAGAAAAAAGACATTTTTCCAAAATCAAGTTAACCGAAAAGAAGATTAACTTTATCTGGCCCTACCAAGAATCTGATTTTGGGAGTGATTCTGAAAAGAAAGATTCAACCACCAAATCATCAGATAATAACCCACGAAAAGGTGATGTTAACGATAAAGACAAACTCTTTTTAGCTCGTAAAAGAGGCATTTTGAAAGCTTACGGGGAAGCCAACATTGCCGAAAAACGGATCTATAAGGGTTTAGCTGTTTCCTTTCAAAATACCTATTCTTTTACTGGAACCGATCAAGAAAGTAATAACTTAAACCAAAATACGGTTTCCGATCCCCAAAACCTGATTTATGACAAAGAAGGTAACCTTTTAGGTTACTTTGTCGATGGTTTAATTCTGGATGGGATTCCCCTGCGCGCTGGGATTGCTCGCTTTCCTGGTGATAAAGGGAAGGGTGAGGATAAGAAAACTACTAAAAAAAAATCAGAAATCAAGCAAGCGAGTAGTGCCACAACAGTTTTACAACCGTTAGCAGCTCAGGCCAAGATGACTGATGCTAAAGAAACCACAAATAACGAGGAGCCCAAAAAAGATAGTAATGTAGAAGAACAATACACTACCAATAATAAAGACAAAGTTTGGTTTAAAAGTGATGAAACTCAAGCTGGTAGTAGCAGTGGAGAAAGCGAAACCAGTAAACTAAGTACCTCTTATCTATTTACCGGAGGGCAAGAAGCTGCTAACTGGTTTCCCAATCTGTACGCTAATGTACCTATTGTGCTCCCGATTAGTCCGGGTTCCCAGTTTTGGTTAGAGACTAATCCCTTTAAAGAAATAATAGAGGTCTTCCAAAAAGAAAAAGAGGAAAAAGAAAAACAATCTTTCTCCTTAACTTTTACTTTAGATACAAGTAAGTTATCTCATTTAGATAAGGAAGAATTTGACTGA", "aa": "MFSFFKQIFKSLKKFFFLLFGIIFVLFSIIFLETSILQLSNNLVNTYTALVQKTNSSDIVAPAIFKESSPVYKTELKEEKRHFSKIKLTEKKINFIWPYQESDFGSDSEKKDSTTKSSDNNPRKGDVNDKDKLFLARKRGILKAYGEANIAEKRIYKGLAVSFQNTYSFTGTDQESNNLNQNTVSDPQNLIYDKEGNLLGYFVDGLILDGIPLRAGIARFPGDKGKGEDKKTTKKKSEIKQASSATTVLQPLAAQAKMTDAKETTNNEEPKKDSNVEEQYTTNNKDKVWFKSDETQAGSSSGESETSKLSTSYLFTGGQEAANWFPNLYANVPIVLPISPGSQFWLETNPFKEIIEVFQKEKEEKEKQSFSLTFTLDTSKLSHLDKEEFD", "aa_hexdigest": "4ae3a448d49e9504b74a9bd5ccda0ab1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 44161.13900000007, "isoelectric_point": 6.816779899597168 }, "id": "APKECPOBEN_1433", "locus": "GCA002090215_01433" }, { "type": "cds", "sequence": "contig_1", "start": 812593, "stop": 813258, "strand": "-", "frame": 1, "gene": "lolD", "product": "Putative ABC transporter ATP-binding MG468-1-like protein", "db_xrefs": [ "COG:COG1136", "COG:M", "GO:0005524", "GO:0016887", "SO:0001217", "UniRef:UniRef50_Q50316", "UniRef:UniRef90_Q50316" ], "nt": "GTGACCAATGGGATCACAAACCAGTTAATCTGTGATCACATTGATCTCAAAATTGCCCCTGGGGAGTTTGTGGTTATTTTGGGTAAATCTGGTTCTGGTAAGACTAGTTTACTCTCTTTAATCTCCGCCCTAGACCGACCTACTAGCGGGGTGAGTTTTGTGTGTGGTAGGAGTACTATTTGTTGTAATGATGCCCAATTAACATCACTACGGAATAAAAACGTAGGTTATATATTCCAACAGTATGGCCTTTTAAGGGATCTGAACGTTGATGACAACATTAAACTGGCAGTTCCCTTTAAAAAGCGCCATAACAACAATTTGGAAGAGCTGTTAGAGCGGTTGGAGCTCAAAGAGCACCGTAATAAAAAGATTACCAAGCTCTCTGGGGGACAACAACAACGGGTAGCAATTGCGCGGGCACTAATCAAAGAGCCGCGTATTTTATTTGGTGATGAGCCAACTGGTGCTGTTAACGTGGATATTTCCAAAAAGATCCTCCAGTTCTTTGTGGAGTATAACCGCGATAAGGGCACTACTATAGTATTAGTTACCCACAATGAAAAGATTGTGGAGCTAGCTAAGCGGGTCATTAAGATCCACGATGGCAAGATTGTGGCTGATTATCTTAACCAAAGACCCAAGACTATTAACGAAATTAACTGA", "aa": "MTNGITNQLICDHIDLKIAPGEFVVILGKSGSGKTSLLSLISALDRPTSGVSFVCGRSTICCNDAQLTSLRNKNVGYIFQQYGLLRDLNVDDNIKLAVPFKKRHNNNLEELLERLELKEHRNKKITKLSGGQQQRVAIARALIKEPRILFGDEPTGAVNVDISKKILQFFVEYNRDKGTTIVLVTHNEKIVELAKRVIKIHDGKIVADYLNQRPKTINEIN", "aa_hexdigest": "9b2835fec29bde0f45f83c1213c1044d", "start_type": "GTG", "rbs_motif": null, "genes": [ "lolD" ], "truncated": "3-prime", "pseudogene": { "start": 812593, "stop": 813441, "observations": { "mutation": [ 813393 ], "stop": [ 813393 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q50316", "query_cov": 1.0, "subject_cov": 0.775438596491228, "identity": 0.995, "score": 428.0, "evalue": 1.3e-150, "gene_start": 1, "gene_end": 221, "reference_start": 63, "reference_end": 283, "reference_sequence": "MSLNAKNKRSLDYCLQWPDFCQSKKASKLIVKLNKKHPKRRXHYKDPEAKHYDVLFKGVCKAVTNGITNQLICDHIDLKIAPGEFVVILGKSGSGKTSLLSLISALDRPTSGVSFVCGRSTICCNDAQLTSLRNKNVGYIFQQYGLLRDLNVDDNIKLAVPFKKRHNNNLEELLERLELKEHRNKKITKLSGGQQQRVAIARALIKEPRILFGDEPTGAVNVDISKKILQFFVEYNRDKGTTIVLVTHNEKIVELAKRVIKIHDGKIVADYLNQRPKTINEINWV" }, "paralog": false, "identity": 0.9929328621908127, "score": 555.0, "evalue": 4.84e-203, "description": "Internal stop codon at 813393. Nonsense mutation around 813393.", "psc": { "uniref90_id": "UniRef90_Q50316", "gene": "LolD", "product": "Putative ABC transporter ATP-binding protein MG468.1 homolog", "uniref50_id": "UniRef50_Q50316", "cog_id": "COG1136", "cog_category": "M", "go_ids": [ "GO:0005524", "GO:0016887" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q50316", "UniRef:UniRef50_Q50316", "COG:COG1136", "COG:M", "GO:0005524", "GO:0016887" ] }, "pscc": { "uniref50_id": "UniRef50_Q50316", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50316" ], "product": "Putative ABC transporter ATP-binding protein MG468.1 homolog" } }, "id": "APKECPOBEN_1434", "locus": "GCA002090215_01434" }, { "type": "cds", "sequence": "contig_1", "start": 813509, "stop": 814828, "strand": "-", "frame": 3, "gene": "dnaA", "product": "chromosomal replication initiator protein DnaA", "db_xrefs": [ "COG:COG0593", "COG:L", "GO:0003688", "GO:0005524", "GO:0005737", "GO:0005886", "GO:0006270", "GO:0006275", "GO:0016887", "KEGG:K02313", "RefSeq:WP_010875043.1", "SO:0001217", "UniParc:UPI0000129530", "UniRef:UniRef100_Q59549", "UniRef:UniRef50_P35888", "UniRef:UniRef90_Q59549" ], "nt": "ATGGAACAATTTAGTGCTTTTAAACTTCTTTTAAAAAAGCAATACGAAACTACTTTAGGTTTTTATGACAAGTACATTAAGAATCTTAAACGGTTTGCTTTAAAAAATAATGTCCTTTTTGTGATTGTGGACAATGAGTTTGCCCGTGAAACCCTCAATAGCAACCCTGATATTGTCGGGTTAGCTAGCAAACTGTACGATGACATTAGAGCAGTTCGGTTTGTCAACGAACAGGACTTCTTTATCAATTTAGCCAAACTAGAGGAAGATAACCGCGAAACGCTTTACCAAAGTTCGGGGCTAAGCAAAAACTTTACCTTTAAAAACTTTGTGGTTGGTGATGGCAACCGCCGGGTTTATGAAGCGGGTGTACGGATTAGCGAAACTCAGGACGCTGATTTCTCCCCCTTATTTATCTATGGGGAGACTGGTCTGGGTAAAACCCACTTACTCCAAGCGATTGGGAATGATAAGTTCTTCCACTTTCCCAACGCTAAGGTAAAGTATGTGGTATCGAGTGACTTTGCCCAGGAGGTTGTCAATGCCTTTTACCAAAAGGAAAAGGATCAAGGCATTGAAAAGCTCAAGGCTGCTTACGAGTCATTGGATTTACTCTTAATTGACGATACTCAGATCTTCGGGAAAAAGGAAAAAACCTTAGAGATCCTCTTTTCCATCTTTAATAACTTAGTAAGTAAGGGCAAGCAAATTGTGTTTGTGTCCGATAAAACCCCCGATGAGTTGGCCAACATCGATCCACGGATGGTATCACGCTTTAAATCGGGACTCTTGTTAAAGATTGAAAAACACAATTTGAGCTCCCTGTGTGAAATTTTGGCCGTTAAGCTCAAAGAAAAGGATCCCAACATCCAAATTACCAATGAAGCACGCGAAAAGGCAGCGCAAATATCTGGTAACGATGTTCGTTCTTTAAACGGGATTGCGACTAAGTTGCTGTTTTATGTCAAAACAACTAAGCAAAACCTGATTAACAACGACAATCTAAAGGAAATCCTGTTTGAGGAATTTGAGAAGTTCCACAAAAAGAGCTTTGATCCTTATTTATTAATAGAAAACGTTTGCCGCCGCTTTAATGTACCGGTGGACAGTGTTTTGTCTGAGAACCGTAAAGCAGAGTTAGTTCGTGTTCGTGACGTTTGTAACTATATTTTAAGGGAAAAGTACAAGATGCAGTTCCAACAAATAGGCAAAATTTTTAAAAGGAACCACTCTACAGTGGTGGCTGCAGTTAAAAGGGTTGCTAAAATGATTGAGAAGGACGACTCCCTCCAAGACATTATCACTTCCTTGGTAATTTAG", "aa": "MEQFSAFKLLLKKQYETTLGFYDKYIKNLKRFALKNNVLFVIVDNEFARETLNSNPDIVGLASKLYDDIRAVRFVNEQDFFINLAKLEEDNRETLYQSSGLSKNFTFKNFVVGDGNRRVYEAGVRISETQDADFSPLFIYGETGLGKTHLLQAIGNDKFFHFPNAKVKYVVSSDFAQEVVNAFYQKEKDQGIEKLKAAYESLDLLLIDDTQIFGKKEKTLEILFSIFNNLVSKGKQIVFVSDKTPDELANIDPRMVSRFKSGLLLKIEKHNLSSLCEILAVKLKEKDPNIQITNEAREKAAQISGNDVRSLNGIATKLLFYVKTTKQNLINNDNLKEILFEEFEKFHKKSFDPYLLIENVCRRFNVPVDSVLSENRKAELVRVRDVCNYILREKYKMQFQQIGKIFKRNHSTVVAAVKRVAKMIEKDDSLQDIITSLVI", "aa_hexdigest": "430c2edf04af85b1c30c24e3e77b7a00", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000129530", "ncbi_nrp_id": "WP_010875043.1", "uniref100_id": "UniRef100_Q59549", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000129530", "RefSeq:WP_010875043.1", "UniRef:UniRef100_Q59549" ] }, "ips": { "uniref100_id": "UniRef100_Q59549", "uniref90_id": "UniRef90_Q59549" }, "psc": { "uniref90_id": "UniRef90_Q59549", "gene": "dnaA", "product": "chromosomal replication initiator protein DnaA", "uniref50_id": "UniRef50_P35888", "cog_id": "COG0593", "cog_category": "L", "kegg_orthology_id": "K02313", "go_ids": [ "GO:0005737", "GO:0005886", "GO:0005524", "GO:0016887", "GO:0003688", "GO:0006270", "GO:0006275" ] }, "pscc": { "uniref50_id": "UniRef50_P35888", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P35888" ], "product": "Chromosomal replication initiator protein DnaA" }, "genes": [ "dnaA" ], "id": "APKECPOBEN_1435", "locus": "GCA002090215_01435" }, { "type": "cds", "sequence": "contig_1", "start": 814828, "stop": 815580, "strand": "-", "frame": 1, "gene": null, "product": "putative protein MPN_687", "db_xrefs": [ "RefSeq:WP_010875044.1", "SO:0001217", "UniParc:UPI000013AFF4", "UniRef:UniRef100_Q50315", "UniRef:UniRef50_Q50315", "UniRef:UniRef90_Q50315" ], "nt": "ATGGCCATCAGTAAAAAGAAACGCTTCTTCTTTGACCTAGCTCAGGATGAAGATGATGCAGAAACTGTTCAAGAAGTTAAAAAGGTAGAACAACAATTAAAACTAGAACCGGTTGTCCAACCCCAACATGATCTAACTAACCAAACTAAAGCAAATCAATCTAGTCAAGACAGAAAGTTCTTTTCTAAGGACATGCCCCAGTTTGATTTTGGACCATTACTCAAGTTTGGCGATGAGTTTGTGAAATCCTTTAACCAGTTTCCCAAACAAGAACCACAAACTTCAACCCAACCAGTTAATGTGCAACCGCAATCAGAACCAACTAACTTTAATAATCAGGTTCCAACCCAACCAGTTCACCAAACTGCAGAAGTGCACTTAAATGAGTTTCAACAACCTACAACCACTAACTTTAATCAACAACCAGTAGCTACATCTAATATTCAGGTGGAGGCTACGCAACCAATTGTGGAACCTGTTCCCCAACCAGAACCACAACCAGCGGTTGAACAGCCACAAGTTAAGCAGACTACTCGCCCATCTAATAAATTGCAGGAAGAAGAAAATCTACCACCACCAAAGGCGAAAGTGCCCGGTATTATTCCCTTAGAACGGCAAGAACGCTTAACCACCGGGGTGCACTTTTATACGAGTACCCGTGTTTGGAATAAGGTTAAGCGTTATGCTAAAGCGGTTAACATTCCGATTTCGCGGATCTTAACGATGATTCTTGATCAGGTAATAGAAGAGTAA", "aa": "MAISKKKRFFFDLAQDEDDAETVQEVKKVEQQLKLEPVVQPQHDLTNQTKANQSSQDRKFFSKDMPQFDFGPLLKFGDEFVKSFNQFPKQEPQTSTQPVNVQPQSEPTNFNNQVPTQPVHQTAEVHLNEFQQPTTTNFNQQPVATSNIQVEATQPIVEPVPQPEPQPAVEQPQVKQTTRPSNKLQEEENLPPPKAKVPGIIPLERQERLTTGVHFYTSTRVWNKVKRYAKAVNIPISRILTMILDQVIEE", "aa_hexdigest": "1fa6f86f9aea8ab7acbbe64c6fd4afe0", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013AFF4", "ncbi_nrp_id": "WP_010875044.1", "uniref100_id": "UniRef100_Q50315", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013AFF4", "RefSeq:WP_010875044.1", "UniRef:UniRef100_Q50315" ] }, "ips": { "uniref100_id": "UniRef100_Q50315", "uniref90_id": "UniRef90_Q50315" }, "psc": { "uniref90_id": "UniRef90_Q50315", "product": "Uncharacterized protein MPN_687", "uniref50_id": "UniRef50_Q50315" }, "pscc": { "uniref50_id": "UniRef50_Q50315", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50315" ], "product": "Uncharacterized protein MPN_687" }, "genes": [], "id": "APKECPOBEN_1436", "locus": "GCA002090215_01436" }, { "type": "cds", "sequence": "contig_1", "start": 815567, "stop": 816379, "strand": "-", "frame": 3, "gene": "parA", "product": "ParA family protein MPN_688", "db_xrefs": [ "COG:COG1192", "COG:DN", "KEGG:K03496", "RefSeq:WP_010875045.1", "SO:0001217", "UniParc:UPI0000131320", "UniRef:UniRef100_Q50314", "UniRef:UniRef50_P47706", "UniRef:UniRef90_Q50314" ], "nt": "ATGATTATTTCTTTTGTTAATAACAAAGGTGGCGTTTTAAAAACAACCATGGCCACTAACGTTGCTGGTTCGCTTGTAAAGTTGTGCCCTGAACAACGGAAAGTTATTTTAGACCTTGATGGTCAGGGCAACGTTTCAGCCTCTTTTGGACAAAATCCAGAAAGATTAAATAACACCTTAATTGATATTTTGTTAAAAGTGCCTAAGTTTAACGGCGCCAACAGTTCAATTGAAATTGATGACTGTTTGTTGCCCGTTTATGAAGGACTAGACATTTTGCCTTGTAACTTTGAGTTAAATTTTGCGGACATTGATATTGCTCGCAAAAAGTACAAGGCTTCTGATATTGCGGAAATAGTTAAACAACTCACCCGCAGGTATGACTTTGTTTTACTAGACACACCGCCTAACATGGCTACCTTGGTCTCCACAGCCATGAGCCTCTCTGATGTGATTGTGATCCCGTTTGAACCTGATCAATACTCTATGTTGGGTTTAATGCGGATTGTCGAAACGATTGATACTTTTAAGGAAAAGAACCCCAACTTAAAAACCATTTTGGTACCAACCAAGGTCAATATGCGCACCAGATTACACAATGATGTGATTGAGTTGGTGAAATCAAAAGCGCACAAGAACAACGTTGCTTTTTCAGAACACTTTGTATCTTTAACATCTAAGTCATCTGCTGCTGTTGGTTATGAAAAGTTACCTATCTCTTTGGTATCACCAACATCCAACAAGAAGTACCAAACTGAATATTTAGAGATTACTAAAGAAATTCTTAACCTAGTCAACCATGGCCATCAGTAA", "aa": "MIISFVNNKGGVLKTTMATNVAGSLVKLCPEQRKVILDLDGQGNVSASFGQNPERLNNTLIDILLKVPKFNGANSSIEIDDCLLPVYEGLDILPCNFELNFADIDIARKKYKASDIAEIVKQLTRRYDFVLLDTPPNMATLVSTAMSLSDVIVIPFEPDQYSMLGLMRIVETIDTFKEKNPNLKTILVPTKVNMRTRLHNDVIELVKSKAHKNNVAFSEHFVSLTSKSSAAVGYEKLPISLVSPTSNKKYQTEYLEITKEILNLVNHGHQ", "aa_hexdigest": "2a80685578b86c1abf65fb4d027b6b5e", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000131320", "ncbi_nrp_id": "WP_010875045.1", "uniref100_id": "UniRef100_Q50314", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000131320", "RefSeq:WP_010875045.1", "UniRef:UniRef100_Q50314" ] }, "ips": { "uniref100_id": "UniRef100_Q50314", "uniref90_id": "UniRef90_Q50314" }, "psc": { "uniref90_id": "UniRef90_Q50314", "gene": "ParA", "product": "ParA family protein MPN_688", "uniref50_id": "UniRef50_P47706", "cog_id": "COG1192", "cog_category": "DN", "kegg_orthology_id": "K03496" }, "pscc": { "uniref50_id": "UniRef50_P47706", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47706" ], "product": "ParA family protein MG470" }, "genes": [ "parA" ], "id": "APKECPOBEN_1437", "locus": "GCA002090215_01437" } ], "sequences": [ { "id": "contig_1", "description": "[organism=Mycoplasmoides pneumoniae] [strain=S63-tet-R] [gcode=11] [completeness=complete] [topology=circular]", "nt": "TATTTACCGACGAAATTAATACCATCAGGGTATTAAGATGCTACCAACGTGGTATTAAAATGTGCCCAACCGCGAAAAAGAAAGTGGTATATAGGAAAATGGAAAACAAATTTGTTTGCCGTTTACTATTAAATACTAATCTTCTATATAGTATAGAGAAACTTTTTCTTTAACATAATATTATCTTAATATTATTTACCTACTAATAGCTTAATATTATTAGTATTTATTTAGTATTATGCTAATACTATGCAGATATTATCTTAATATTATCTATAGTATTAGGCTAATATTATTCTTAATATTTATTAAGGTACTAAAGCATTACCTATAGGTGATATTATGACAATACTAAAGTGGTTAGTATTATTAGGGTATTATTCAAAGTATTCTCCAACACTATTCCCTTAGCTCTAACTTAATATCCCTTTAATACCAAGATGGTTAAACAATGAAGAAAATTAGTTGTTTGTTCTGGCCCTTTACTATATATGTACATAGTACATATATAAGAAAGGCTACCATGACATTATATATAATAGGCATCCAGAAATAAAAGCCAAATTAATCTATAATTAAGTATTTATACTTAATTATAGTTATTTTATTAACAAAATTAGTGACATTTGGCCATCCATAATAAATAAAATCAAATAAACAAAACAAAAAGCAAAAAAACTATAAAAAGGGCATGAAAGTTTTGATTAATAAGAATGAGTTGAACAAAATCCTCAAAAAACTCAACAATGTAATCGTATCTAACAATAAGATGAAACCATACCACTCTTATTTATTAATAGAGGCTACAGAAAAGGAAATTAACTTCTATGCTAACAACGAGTACTTTTCTGCTAAATGTACCTTAGCCGAAAACATTGATGTACTTGAAGAAGGTGAAGTAATTGTTAAAGGCAAAATCTTTAGCGAACTCATTAACGGAATTAAAGAAGACATCATTACTATTCAAGAGAAAGATCAAACTCTTTTAGTCAAAACAAAAAAAACAAACATTAACCTTAACACGATTGATAAGAAAGAATTCCCCAGAATCCGTTTCAACCAAAACGTTGATTTGAAGGAATTTGATGAACTTAAAATCCAACACAGCCTTTTAACTAAAGGACTTAAAAAGATTGCCCATGCTGTTTCTACATTTAGAGAATCCACTAGAAAATTCAACGGGGTTAACTTCAACGGTTCCAATGGTAAACAAATCTTTTTAGAGGCATCGGATTCTTATAAGCTCTCTGTTTATGAAATCAAACAAAAAACCGATCCATTTAATTTCATTGTCGAAACTAATCTTTTGAGCTTCATCAATTCTTTTAACCCTGAAGGTGGTGATTTAATCAGTATCTTCTTCCGCAAAGAACACAAGGATGATTTAAGTACCGAATTACTGATTAAGTTAGATAACTTCTTAATTAACTACACCTCAATTAACGAAAGCTTTCCGCGGGTAATGCAGTTGTTTGACTTTGAACCAGAAACCAAAGTAACCATTCAAAAAAACGAACTTAAAGATGCATTACAAAGAATCTTGACGTTAGCTCAAAACGAGCGCTTCTTTCTCTGTGACATGCAAGTAACCAACTCCCACCTCAAAATTAATTCCAACGTTCAAAACATTGGTGCATCCTTAGAAGAAGTTACTTGCCTTAAGTTCGAAGGTCACAAACTCAACATCGCTGTTAATGCGCTTTCCCTCTTGGAACACATTGACTCATTTGATACTGATGAAATTGAGCTTTACTTCCAGGGCAGTAACAAATACTTTTTAATTAGTTCGAACAACGAACCTGAACTTAAAGAAATCCTAGTTCCTTCCAAGTAACTAATGACGCTTTATGACTTACTAGAGCTGCCCCAAACTGCTACACTACAGGAAATCAAAACAGCTTACAAACGCTTAGCTAAACGTTATCACCCTGACATCAATAAACAAGGGGCAGATACCTTTGTCAAGATTAATAACGCCTATGCCGTTTTAAGCGATACCACCCAAAAAGCGGAGTATGACGCTATGCTGCGCTTTAGTGAATTTGAAGACCGAGTTAAACGCCTGGACTTCTCGATTAAGTGACACGAACAGTTTATGGAGGAATTACAGTTCCACCACAACTGGGACTTTGACTTTATCCGCAACAGGGAATACACCCAGCCAACCCCTACCAACAATAAGTATTCTTCCTTTTTAGACAAGGATGTATCCTTGGCCTTTTACCAACTTTACTCCAAGGGCAAGTTGGACTTTGACTTGGAAGACACACTTCTTAGACGTCATTCGATCAAGCAAGCCTTCTTAAAGGGCAAGAAGCTTAACGATGTTTTAAAAGAGCAGTACAACTATTTAGGTTGATTAGAGGCCAAGCGCTATTTCAACATTGATGTCGAAATCGAATTAACCCCCAAAGAAGTGCGTGAAGGTGGGGTGGTCAACCTCCCTTTAAAGATCAAAGTCATTTCCAATAACTACCCTGGACAAATGTGGTACGAACTCAACAAGAACTATTCCTTCCGGCTTCTTTGAGACATTAAAAACGGTGAGGTGGCAGAGTTCTTTGGCAAGGGTAATCGGGCCTTGGGTTGACGCGGTGATCTGATTGTGCGAATGCGGATTGTCGACAAAATTAAGAAGCGCTTACGCATCTTTTCCAGTCATTTTGAACAGGACAAAACCAAGTTGTGATTCCTCGTGCCGCAGGACAAACAAGACAATCCTAATAAGTGGGTTTTTGACTATAAAACACACGAGTTTATTGTTTAAAATCGCTTAATTTTTAATGTGGTATAATTGTTTGGATTCGCCATAAATTTTGCTTATGGCTTTTAACTTTCCAACGTGCACATTAAATGGAAGACAATTAAATGGAAGACAATAACAAAACGCAAGCTTACGATTCCAGTAGCATTAAGATTCTTGAAGGCTTAGAGGCCGTGCGTAAGCGCCCCGGCATGTACATCGGTTCCACCGGCGAAGAAGGGTTGCACCACATGATCTGGGAAATCATTGATAACTCAATTGACGAGGCCATGGGTGGCTTTGCGAGTACTGTCAAACTAACCCTTAAAGACAACTTTGTCACCATTGTGGAGGATGATGGCCGGGGCATTCCCGTTGACATCCATCCTAAGACCAACCGCTCCACCGTTGAAACAGTGTTTACGGTACTCCACGCTGGGGGTAAGTTTGACAACGATAGCTATAAGGTGTCAGGGGGACTCCACGGTGTGGGTGCCTCGGTTGTTAATGCTTTAAGTTCCTCCTTTAAGGTATGGGTAGCCAGGGAGCACCAACAGTACTTCCTAGCGTTCCACAACGGTGGGGAAGTGATTGGTGATCTTGTCAACGAAGGTAAGTGTGACAAGGAACACGGTACCAAGGTTGAGTTTGTCCCGGACTTTACCGTGATGGAAAAGAGTGATTACAAACAAACGGTAATTGCTAGTAGGTTGCAACAATTAGCCTTTCTTAACAAAGGGATCCAAATTGACTTTGTTGATGAACGCCGTCAAAATCCGCAAAGCTTTTCTTGGAAGTATGATGGTGGCTTAGTCCAATACATCCACCACCTCAACAACGAAAAGGAACCTTTATTTGAGGACATTATCTTTGGTGAAAAAACCGATACTGTTAAATCAGTTAGCCGTGATGAGAGCTACACAATTAAGGTGGAAGTGGCCTTTCAGTACAACAAGACGTATAACCAATCAATCTTTAGTTTTTGTAACAACATTAATACCACTGAAGGCGGTACCCATGTTGAAGGCTTTCGCAATGCCTTAGTGAAGATCATTAACCGCTTTGCTGTCGAAAACAAGTTCTTAAAGGAAACGGATGAAAAGATTACCCGCGATGACATCTGTGAAGGGTTAACGGCCATTATCTCAATTAAGCACCCCAACCCCCAGTATGAGGGTCAAACCAAAAAGAAATTGGGTAACACCGAAGTGCGTCCTCTAGTTAACAGTATTGTTAGTGAAATCTTTGAGCGCTTTATGTTGGAAAACCCCCAGGAAGCCAACGCCATTATTCGCAAAACGCTGTTAGCGCAAGAAGCACGGCGCCGTAGTCAGGAAGCACGGGAACTGACCCGCAGGAAATCTCCCTTTGACAGTGGTTCACTCCCGGGTAAGTTAGCAGACTGTACCACCCGTGATCCCTCAATTAGTGAACTGTACATTGTCGAGGGAGATAGCGCTGGTGGTACGGCTAAAACGGGGCGGGACCGTTACTTCCAAGCGATCCTACCATTAAGGGGTAAGATCCTCAATGTGGAAAAGTCCCACTTTGAACAAATCTTTAATAATGTGGAAATCTCGGCTTTAGTCATGGCCGTTGGCTGTGGCATTAAACCAGACTTCGAGTTGGAAAAACTGCGGTATAACAAGATCATTATCATGACCGATGCTGATGTCGATGGGGCGCACATCCGTACCCTCCTTTTAACCTTCTTCTTTCGCTTTATGTATCCCTTGGTGGAACAGGGCAACATTTACATTGCCCAACCCCCACTGTATAAGGTCTCTTACTCTAATAAGGACTTATACATGCAAACCGATGTCCAACTAGAGGAGTGGAAGCAACAACACCCTAATCTGAAATACAACTTACAACGCTACAAGGGTTTAGGGGAAATGGATGCCATCCAACTGTGGGAAACCACGATGGATCCCAAGGTGCGTACCTTACTAAAGGTAACCGTTGAAGATGCTTCAATTGCTGATAAAGCCTTCTCCTTGTTAATGGGCGATGAAGTTCCCCCAAGACGGGAATTCATCGAACAAAACGCACGTAACGTAAAGAACATTGATATCTAATGGCAAAGCAACAAGATCAAATTGACAAAATTCGTCAGGAATTAGCACAATCAGCAATTAAAAACATCTCCCTCTCCAGTGAACTAGAACGTTCCTTCATGGAGTATGCGATGTCGGTGATAGTGGCTCGCGCGCTCCCAGATGCGAGGGATGGACTTAAACCTGTGCACCGCCGTGTGCTCTATGGTGCTTACACTGGGGGCATGCACCACGATCGCCCCTTTAAAAAGTCAGCACGGATTGTCGGTGATGTAATGAGTAAGTTCCACCCCCACGGGGACATGGCCATTTACGACACAATGTCGCGGATGGCGCAGGACTTTTCTTTGCGTTACCTCTTAATTGACGGACATGGTAACTTTGGCTCGATTGATGGCGATCGTCCCGCAGCACAACGTTATACGGAAGCACGGTTGTCGAAATTAGCGGGGGAACTGTTACGTGACATTGACAAGGACACGGTCGACTTTGTCGCTAACTATGATGGGGAAGAACAAGAACCAACGGTTTTACCAGCTGCCTTTCCTAATTTATTAGCTAATGGTTCGAGTGGGATTGCGGTAGGGATGTCCACTTCCATTCCGTCTCACAACTTATCGGAGTTAATCCAGGGCTTAATCCTTTTAATTGACAACCCTGACTGCACCATTAATGATTTATTAGGTGTCATTAAAGGACCGGACTTTCCCACCGGAGCCAACATTATTTACACCAAGGGGATTGAAAGCTACTTTGAAACTGGGAAGGGTAATGTGGTCATTCGTTCTAAAGTCAGCATTGAACAACTACCAACCCGGGCTGCTTTAGTAGTCACGGAAATTCCCTACATGGTCAACAAGACCAGTTTAATTGAAAAGATTGTCGAGTTAGTCAAGGCTGAGGAAATCACCGGGATTGCCGATATCCGTGATGAGTCATCCAGGGAAGGAATTCGCTTGGTGATTGAAGTGAAGCGCGACACCGTTCCGGAAGTCTTATTAAACCAACTGTTCAAATCAACCCGTTTACAGGTGCGGTTCCCCGTGAACATGTTGGCACTGGTTAAGGGTGCACCTAAATTGCTTAACATGAAGCAAGCGTTAACGGTTTACCTCGAGCACCAACTCGATGTTCTGATTCGCAAAACCCAGTTTAACCTCAAGAAGTACCAGGAGCGCTTTCATATCTTAAGTGGCCTTTTAATTGCGGCGTTGAACATTGATGAGGTAATTGCGATCATTAAAAAGTCCGCTAATAACCAGGTAGCAATGGAAGCACTCCACGAACGCTTCGGGTTAGATGAAATCCAAGCACGGGCGGTTCTGGACATGCGTTTACGTAGCTTAAGTGTCTTGGAAGTCAATAAACTACAAACCGAACAACAAGAGTTGAAAGCTTTAATTGAGTTCTGTCAACAGGTGTTAGCTGACAAACAACTGCAACTCAAACTAATTAAGGAGCAGTTAACCAAGATTAACGAACAGTTTGGCGATCCACGCCGTAGTGAAATCCTGTACGGTATTAGTGAAGACATTGATGATGAGGACTTAATTACCCAGGAAAACGTGGTAATCACGATGTCCACTAACGGTTATCTCAAACGGATTGGGGTAGACGCTTATAACCTGCAACACCGTGGTGGGGTGGGTGTTAAGGGTTTAACTACCTACACCGATGACAGTATTAGCCAGCTGTTAGTCTGCTCGACCCACTCGGACCTCTTATTCTTTACGGACAAGGGAAAGGTCTACCGCATCCGGGCCCACCAAATTCCCCCGGGTTTTCGTACTAACAAGGGGATTCCGGCGGTTAACCTGATTAAGATCGACAAGGACGAGAAGATCTGTGCCTTAATTTCGGTGAATGATTACCAAAATGGTTACTTCTTCTTTTGTACCAAGAACGGTACCATTAAGCGCACCAGTCTAAGTGAGTTTGCCAACATCTTGAGTATTGGTAAACGCGCCATCCTCTTTAAGGAAAACGATGTTTTGTTCTCGGTTATTCGCACCAGTGGTCAAGATGACATCTTTATCGGTTCGACCGCGGGGTTTGTCGTGCGTTTCCACGAAGACACCGTCCGTCCGCTGTCCCGCGCTGCCATGGGGGTCTTGGGGATTAATCTCAACCAGTGCGAATTTGTCAATGGTCTGTCCACTTCCAGTAACGGGAGTTTATTACTCTCTGTGGGTCAAAATGGCATTGGTAAGCTCACCAGTATTGACAAATATCGTTTAACCAAGCGGAATGCTAAGGGAGTTAAAACACTACGAGTAACTGCTAAAACTGGTCCGGTTGTGACCACTACGACCGTCTTTGGTAACGAAGATTTGTTAATGATTTCTTCAGCTGGTAAGATTGTCCGGATTAGTCTGGAACAACTTTCAGAACAGGGAAAAAACACTTCTGGTGTTAAACTAATCAAACTAAAAGAGAAGGAACGCTTAGAGACTGTTACTATCTTTAAAAAGGAAGAAGCCATTAAAACCACAACAGCAACCGAGACAGACGATGTTGGATCGAAACAAATTACGCAATAACCTTGACTTCTTTAAAAAGAAGTTAGTTGAACGGGGTGTAAGTGAAAGCCAGTTCGAGGCGTACGTGCAAGCTGATAAAGCAATGCGCAAACTGTTGCACCAAATTGAACTGGCCAACCAAAAGCAAACCCTGTTAGCGCAACAGGTTGCCAAGAAGAAGGGTGATCCGAAGTTATTAAAGGAATCCAAGGAGTTAAAGCAAAAACTAGAACAGCTCAACATCGCTTTTAAGGAAGCGGAAACCTTAAGCCAAGAGTTACTGTTAAACCTCCCTAACATTGCTGATGAATCAGTACCAGTTGGACGGGATGAAACTGCTAACTTAGAACTGCTCAAAGAAGGGCGTAAACCGGTGTTTGACTTTACTCCCTTACCCCACTGGGAGTTGTGTGAACGCTTACAGTTAGTTGCCTTTGATAAGGCCACTAAGCTAACGGGTGCACGGTTTGTCGCTTACACTGATAAAGCAGCGAAACTGCTCCGCGCCATTGCTAGCTTAATGATTGACCTCAACAAAAACAAATACCAGGAGTGAAACGTGCCGGTCATTGTGAACGAGACTAGTCTTACTGGTACGGGTCAGCTACCCAAATTTAAGGATGATGTCTTTAAGTTAGAAAACACGCGTTACTACCTCTCCCCGACCCTAGAGGTGCAACTAGCCAACCTCCATGCCAACGAAATCTTCACGGAGGGTGAACTCCCCAAATACTATACCGCTACTGGGGTGAACTTTCGCCAGGAAGCGGGCAGTGCTGGTAAACAAACTAAGGGTACGATTCGCCTACACCAGTTCCAAAAGGTAGAACTAGTGAAGTTTTGTAAACCAAGTGAAGCGATTCACGAACTGGAAGAAATGACCCGGGACGCTGAGCAAATTTTGTTAGAACTCAAAATTCCCTTTCGCCGTTTGTTACTCTGTAGCGGTGACATGGGTTTTAGTGCCCAGAAAACCTATGACTTGGAAGTGTGGATGGCGGGTTGCAATGAGTACCGCGAAGTTTCGTCCTGTTCTTCTTGTGGTGACTTTCAAGCACGCAGAGCAATGATCCGCTATAAGGACTTAACCACAGGCAAGAATACCTATGTGGCTACCTTAAACGGCACCGCTTTAGCGATTGACCGCATCTTTGCAGCCATCTTGGAACACTACCAAACGAAGGCAGGGGAAGTAATGATTCCCCAAGCCTTGTTGAAATACTTAGACTTTGACAAAATTACCAAACCAAAATAAACCAATGAAACAAGGCGTGTTTGTGGCCATAGAGGGCGTGGATGGTGCGGGGAAAACCGTCCTCCTAGAAGCTTTTAAACAGCGCTTTCCCCAAAGCTTTTTGGGCTTTAAAACGCTTTTTAGCAGGGAACCCGGGGGCACACCGTTAGCGGAAAAAATCCGGGCACTGCTCCTGCATGAAGCAATGGAACCACTTACAGAAGCTTATCTGTTTGCGGCATCGCGCACCGAACACGTGCGCCAGTTAATCCAACCAGCGTTACAACAAAAACAGTTAGTGATAGTAGACCGCTTTGTGTGATCTAGTTATGCATACCAGGGCTTAATTAAAAAAGTCGGACTTGATGTGGTCAAAAAGCTCAATGCTGATGCGGTGGGGGATAGTATGCCCGACTTTACCTTTATTGTTGATTGTGACTTTGAGACTGCTTTAAACCGGATGGCCAAACGCGGTCAGGACAATTTATTGGACAATACTGTTAAAAAGCAAGCGGACTTTAATACTATGCGCCAGTATTACCACAGTTTGGTTGATAACAAACGCGTCTTTCTCTTAGACGGGCAAAACCAAACGGGTTGTCTGGAACAATTTATTGAACAATTAAGCCAATGTTTAACCCAACCCACGCTCTCTTAATTATTCAAAGACGGGGTAGCTATCTCCAACCAGTTTTAACGGAGTACCTAACCCGGGTCGTTTGTGAACAGCAAACGGGGTGTCAAACATGTCCGAGTTGCTTGGAAATTCTCCATGGTACTTACAACAACTTTTACAGTTTTGACCAAGCCAACCCCTTTAAAAGGGAACATGCCTTGCACCTTAGTGAAGTGTTGAACCGCCAAAGTGAATCCAACCAAAAACAGCTGTACCTGATTAAAAACTTAGAAACGCTCACCGCTACGGCCATGAACAGCTTGCTCCGTTTAATTGAGGAACACCCGGTGAATACCTATGGTGTTTTTACCACGAAAAACGAAAACATGATCTTACCCACCATTTTGAGTCGCGTCCAAAAAGTAGTACTCAAAAAAGCGACCCAGTTACCGTTCCAAGTGGACAGTAAGGACCAGGCGATTCTCAAAAGTTTCTTTAGTGTAGATGAACAGTTACAAGCATTGGACAACGGTAGTTTTACCCGCTTAAAAACGATTATTACGACACTTACCAATAAGAAAAACACAGCGAGCACGGTTCACGAGGCTTGGGTGTTGTTTAAACAATTAAACCAAACGGAAACGGCTCAAGTACTGAACTTTATGGTGGATTACACCAAGGACTTAACCAAAAAGGACCGCTTGTTAAACATGGTGCAAAATTTGGTGTTTAACCCACCGAAAGCAGCGTTGTTTGCTAACCTCATTAACTGATAAGATGGACACCAAACAAACCATGTTTGCCCTCGCTACTGCGCCCTTCAACAGTGCCATTCACATTATTAGGTTATCTGGTCCCGATGTTTATCGCATTATTAACCAAATAACCAATAAGGAAGTCAAACCGTTGGGTATGCGGATTCAACGCGTTTGGTTAATTGATCACAACCAGAAAAAAGTGGATGATGTGTTGCTGTTTAAATTTGTCGCACCTAACTCTTACACTGGGGAAGATCTGATTGAAATTTCGTGCCATGGCAGCATGGTAATAGTCAACGAAATTATTGGTTTATTGTTAAAGCACGGGGCGGTACAAGCCCAACCCGGGGAGTTTACGCAGCGCGGCTATCTCAACGGTAAGATGAGTTTAAACCAAGCGGCTTCTGTTAATAACTTAGTACTTTCACCGAATACAACCCTAAAGGATGTCGCTTTAAATGCGTTAGCTGGTCAAGTTGATGCCAGGTTAGAACCCTTAGTGGAAAAGTTGGGTCAACTAGTAATGCAAATGGAAGTGAACTTGGATTACCCCGAGTACACTGATGAACAGCGCGAACTGGTGACAATGAACCAAGCAGTCGTGCAAATAACCCAAATCTTAAACCAAATCGTGGTTGGTCAAGACCAGTTACAACGGCTCAAAGATCCGTTTAAGATTGCCATTATTGGTAATACTAATGTTGGTAAGTCCTCCTTATTGAATGCTCTGTTAGATCAAGATAAAGCGATAGTATCCGCCATTAAAGGCAGTACCCGCGACATTGTTGAGGGTGACTTTGCTCTAAACGGTCACTTCGTCAAAATCCTCGACACAGCGGGAATTCGCCAACACCAAAGTGCGCTCGAAAAAGCGGGCATTCAAAAAACCTTTGGCGCCATTAAAACCGCTAACTTAGTAATTTACCTTTTGGATGCCCGGCAGCCAGAACCTGATCCCAAGATTATTGCGCGATTAAAGAAGCTGAAGAAGGACTTCTTTTTGGTCCACAATAAAGCAGACTTAGTGCAGCAAAGCTTTCAGGTAAGTATTTCTGCCAAACAAAAACAGATCCAACCACTTGTCGATTTATTAACCCAATACCTACACCAGTTTTACAGTGTGGAGCAGAACCAGCTCTACTTAATTAGTGATTGGCAAACGATCCTGCTCCAAAAAGCAATAGCCGAACTAGAACATTTTTTAATTAAACAACAAAACTGCCTTTTCTTTGATATCCTAGTTGTTCATTTACGTGCTGCTCACGAATACATTCTCCAAGTCTTGGGTAAAAACACCAACTATGACCTGATTAACGAAATCTTTAAGCACTTTTGCTTAGGTAAGTAATGGAATACTTTGATGCGCACTGTCACTTAAACTGTGAACCCCTACTGGAACAGCACGAAAAGAGTTTGGCCAACTTTAGGTTAATTGGGTTAAAGGCCAATGTTGTGGGCACTAATTTAACTAACTCCCAAATTGCGGTGAATTTAGCTAAACAGCACCCTGATTTACTCAAGGCAGGGGTGGGTATTCACCCTAATGATGTCCAGCTTTTTGATTTAAAAGCAGCGCAAGCAACACTAAAAAAATTAGTAAGTACGCATCGCTCATTCATTAGTTGTATTGGTGAATATGGCTTTGATTACCACTACACTAAGGACTACATTACCCAACAAGAACAGTTCTTTTTGATGCAGTTCCAACTAGCAGAACAGTACCAGTTAGTGCACATGTTGCATGTCCGTGATGTGCACGAAAGGATATACGAAGTTTTGAAAAGGTTAAAACCTAAACAACCAGTGGTGTTTCACTGTTTTAGTGAAGACACCAATACTGCTTTAAAGCTGCTAACTTTAAGAGAAGTAGGACTAAAGGTTTATTTTTCGATTCCGGGGATTGTGACCTTTAAGAATGCGAAAAACTTGCAAGCCGCTTTATCAGTTATTCCAACAGAGCTACTTTTAAGTGAAACCGATTCGCCTTATTTAGCACCAGTACCTTTTCGGGGCAAAACTAATTGACCGGAATGCGTTGTTCATACAGTGCAAACGATTGCTGACATTAAGCAAGTTCCTTTAGCTGAAATAAAACAAGCGATTGTGCATAACGCCAAGAAGTTATTTTGGTAGTCCTTAGGATTAACTTTTAAAAGTTCTTCTTTTGTTGGAACCTTTTCACAACTTATTCAATTTATTTAGTGTAGACATGAATAAAAATGCATACACTAGAAAAAATTAGTTCCTGATTAAAGGGGAATCCTTATTTAAACAAAATTGTAAGTAGTAGTTTTCTCAACCTCTTTAAAAAGAAAGGAGGTATTGCTTAATGGCTTACTCGCCTTCGCTTAACGACATTAAGAGCATTTTGAACAAATATACCTCTAAGGACTATGAGTTAAAATGCGAAAATAGGTATGATGGCAAGCTTGAATTATGATTAAAAGGAGTTTTTGAAGAAATCGTGAAAACACCTGGCACTAGGTACGTCACCCATAAGCAACTTGATGAAAAGCTCAAAAATTTTGTCACTAAAACAGAATTTAAAGAGTTCCAAACTGTAGTTATGGAGAGTTTTGCTGTTCAAAACCAGAATATTGATGCTCAGGGTGAGCAGATCAAAGAACTTCAAGTTGAACAAAAAGCTCAAGGAAAGACCCTGCAATTGATACTTGAAGCTCTCCAGGGTATAAACAAACGTCTAGACAACTTAGAGTCCAAATAAAAAACTAAAGCAGTTTTTATACCAACAGTTGACTCTTAAAAAGTTTGCTGTCGGTATTTTTATCCACTTCTAACAAAAAATCATTAAAAAACAGGCGACTAAAAAATCTTGAAGAACAACGTTAGGTTGTTCAAAGAATTTTGAACAATAAAAAACAAAACTAAGTTGAATTTTTAGTTAAGCCATTACTCAATTAAATAGACTAAATATCTGAATAGATTAATTTGGTAGTTATCAACTATGTATTCAATATAACCATTAAGAACTGTTTTGTCAAGAATTTTTTGACTAAGTTCATCTTTGCCTTTTAGTATTCAATGAAACGAAACAGATAACCTAATTCTTTTGGAATAGACATCAAAATTTTTTGACGTAAATGGTAAATATGAATTCGCTTTAAGAGTAAGGAAACTGTTGAAATAATGTTTGTCACCTTTGTGGATATTGAATCCTCTGTAAGAATAATTTTCATTTCATGTGAGAGTATGGTTCAACTTGGCTTTAACTGAAACATCGCTAATGCCTTTGTATGTCATGTTGAAATACTCAACTTTTTGTTTCTTATTAAAAGTATTAACGCCTGAAAAAATCGATTTAGTTCAATTAAATAAATCAATACCGATTTCATTTTTTTCTGCACTTTGCTTTCAGGCATTTAGTAATAGTTCAGTAGTTTTTTCAGAGTTAACTTCGTAAGATTTTTTTAAATAACCTATTAAGATTTGTTTCCCTTCCGAAGTAATAATTAATTTAGAAACATCAAGGTCTTTACCATCTGTTTCCTTAATAAGATTTTTGAGATCAGCTTCGAAAGTGGCGCTACAAGCACTAAGAAATAAAACTGAAGATAATAGTGGGGTTAATAAGAATTTAAATTTCATTAGCGCACGTAGTAAATTACTTAATTAAGAAAAGCAAGTATCTAAAGAGATTAACTGTGTATTCAGAATTTGTTCTAGTATCATAGCCTTCCAAAAAGATTTTCTTAAGATCTCTTTGACTTTGTTTATCTTGCCTTGCAATGATCAACTAAGTGAAACAGGGAACTCAATTCATTCTGTGTCAACGCCTTCCCCTGTCTTAGTAATGAAACTTATCGTTTTAGATTGGTTCTTTGAAGATGGCTTAATTGCAAGAGGAAGATCAAAAACATGTTTGTCACCTTTTTTGGCAGCAAACCCATTGGAAGAATAATTTCCATGCCAATTAAAGTTATAGTTCAACTTAGCATTTATCGAAAAATTATTAAGATCTTGATATTCCATATCGAAATATTCAACTTTTAGTTCGATTTTTAAAGGATCATTGCCTGTTGCATAAATACCAACATCGGGAAAATTAATTTTCTCAATATCTATTTCACCTTTTTCCGCACTCTGTTTTCAGGCATCTAACAATAGTTTTGTGGTTTTATCAGGATTAACTTCGTAAGATTTCTTTAGAGAAGTTATTAAGTTTTTTCTGCCTTCAGAAGTTTTGATTACTTTGTACACATCAACGTCTTGTTTAGTGGTTTCTTGAATAAGATTTCTTAGATCAGACTGGATAGAAGCATAAGCACTAAACAATAAAGTTGAAGATAAAAACGGAATTGAAAGGTATTTAAATTTCATTGAGGTACAGAAAAACTAACTATGGCAATAGAACCTTTTTGGTAATGGTAAATTTTATCTAGTGTTTTATTGTTTTTCAACTTAATTCAGTAAAAGCATCCACATCTAAATAAAAAAGCCATCTGTTTAGGGCGAAAAGCAGATGACTAAAAATAATTTTTTAACGATTTAAAAGCTAACGGAGATTGTTAGTTTGATGTATAAGTAAAAATATGTTTTCTAAAAATTTATTAAGTTGGCCGGGAGCAGTAAAAACTTTTGATTCCAGCAGCTCTTCCTGCTCCCACTAAATAAATTGACTTGGTTTTACAAAGACGCCAAAAAGTTTGGAGCTTTTTGTAATTTAGTTTTTTAGCTTCTTCTAACTATTAAGAAATATTTACTTTTCTCAAAAGCTTTTGAAAGGTTTTTGTCACTTCGCTATTTTTAAAGGCATTTAAGAGATGTAAATACATCGCTTATGTTTCTAAATACTCTTAAAAATTTATTTGGGTGACTTGGATCGCTCTTGATGTTGTTTTGTTTGAAGTCTCTTTACTTTAAATTGCAAAAATTTTTAGCAATTTTATTGGCAGTTTTTGAAATTCTGGTCAATTTATTTAGTGAAGGAAAAATTATGTCTTTACTCAATATATTTATTAGATCAACGATGATGTTGATCCTTGCTGGCAACAAAGTTGCACGTTTTAGTTTTCTAAACCTTGAGCAGAAAGGAGGAATTTATTAATGCCTAAAGTTATTTCCCAAAAGGAATTTAATATCCTTTCCCGGACAAAGGAAAGAGTTAATTTTCAAATTGTCTATACCAAGCAAAGAAATGGTTTGCACAAAGCTGCTAAGGAGTACTTTTTTAAAGACAAAGACTTTACAATAATAGAAGATACACCGGACACACCTGACCAACCAGATGGACCATGTATTACTAAAGGCCCAAAGGAACCAGGTGGTCCTGAAGAGCCAAATGGTCCGGATAAACCAGAAGATCCTAAAGATCCTGATACGCCAGATGTTTCTGAAGGCCCTAAAGAACAAGGTGGTCAGAAAGATCCAGGTGGTCCGGATGATCCCAGTGAAGGAAAGCAGAAAGTGTCCAAACCAGATAAATACGTAACCCACCGTGAGCTTGATGAAAAGCTTAAAGACTTTGCCACCAAAGCTGATTTCAAACGCGTCGAAGACAAAGTTGATGTTTTATTTGAACTGCAAAAAACGCAAGGCGAACAAATAAAGGTTCAAGGTGAGCAGATCAAAGCTCAAGGAAAACAAATTGAACAATTAACTGAAACTGTCAAAGTGCAAGGCGAGCAGATTAGGGCTCAAGGAGAACAAATCAAAGCTCAGAGCGAAGAAATAAAAGAAATTAAGGTTGAGCAAAAAGCCCAAGGCGAGCAAATCAAAGAACTTCAAGTAGAACAAAAAGCCCAAGGTAAAACCCTACAGTTAATTTTAAAGACGCTAGAAAAAATGAACGAGCGTCTAGAAAAATTGGAATCTAAATAGATAACAATCAAAGCAAAAATACCAGCAGTTGACCCTTATAGGTTATCTGTTGGTATTTTTATTTTGGCGCTTACTAGTTCTTGTAACCATTTATTAGTTAAATGGAACTTAGAAATACAAAATTAGAACAACTCTTTTACATCTATCACCGCAACCTGAAGTATAGATGTCCAATGCAGTACCTCACTAAAAGGGGCTTTAACTTACAAGACCTCTTGTCAGTTGGTGGTGGATTAGCTTACTTGGGCGAAAAACAGTGGTTAAATTTATCACTGTACAACTTTAACAACCAGTTAGTTGGTTTTTTAAGTCGGAAGGTAGGTTTTGAAAAGAAGTTCCTTTATTTACCTATTAATAAACCACCAAGTAAAAGTGAATCTTTTTTAGGTTTAAAACACTTACCAACGGAAACTAACACCATTTACCTAGTTGAAGGTGATTTTGATTGGTTAGCTTTCCGTAAGGGTGGTATTTTGAACTGCTTGCCTTTATGTGGATTAACTTTATCAGATAAACAAATGAAGTTTTTTCAACAAAGTAAGATCGAAAAAGTGGTGCTTTGCTTAGACAATGATTTTGCTGGTAAAGTAGCTGCTGCTAACCTGGAACGCATTTTAAAAAATGCTGGTTTTCAGGTTAAGGTAGTTCAGCTAAAGGGTAAAGTTAAGGACTGAAATGAATTATTACTACTTTACCCGAAAAACTGAGCTAAAGCCTTAAGGGATCATTTGGCGCTTTAAATGATGTTTAAAGTGAGTTAAGGACGATTTAATAATTACCCTTGGTAGGTTTAAGTCAGGACACATTTTGGTTAGTGCCCTAGCACCAGCAGCATCCTCGATTTCATTGACCACTATTCGGTTGTACTTATTTAAGAAGAAGTCAATGGCGTAGTAACCATGACCTAGCTTTTTAGCAATCCGTTTAATTTTGTGCTTTATGTATCACTTTAGTTTAAACAAATCAACAGCAGCATTTTGTGAGTAGTTAGCCCTAAAATCATCACCACTAGCAGTCCTTCTAATTGTTTTTAACACCTTACCAAACAAGATGTAAGCCCGGTATTCCACTTTACCAATTGACAAAAATGGTTGGACAATCCACTCATTAGGATTTTGAATTGGTAACTGCTTAATTTCTTCCGCATTTTGAATTAAGTGCACATCTTCCCCACCATAAGAATCACGCTTTTTGATCACAATCGGAAAAGTCTTAATCTTTTTGGGATCAAAAATGGTGGTATCAACGGTCAAGAACTTATGCTGCTTTAACCACCTGTAAGTTTCATACTTATCATTGGCTACCAAAACGATATTTTGGGGATTAATGAGAAAACTGTTGAGAAAGTTGTGTTTGGTTAAAAAATCAATTCTGCGCGAACGGTTAATGATCAATTCCACATTTTTAATGTGTTTGTGATCAGCGGTTTCATCATCTAACAGCAAAATATGCCCAACTAGTTTTTTCTTGTTCAGTTCATCGACTAGTGCTTGAGCAAAGTGCTTGTTCTTGTCAAAATCGGTCTTATTGTAAACAACGAGTGCTTTTCTGTTTTTTAACTTAATTTTGGCCATTATGCGATTGATGCTAAAAGTTGAATGGCGAGGTTAGTTTTCAAATACATCGAGCTTTTGGTGAGCTGTACATTGGAATTAACTTCGCAGAAAATTAACGAACGATCCTGGTCAAATAAAAAGTCAATGCCACAATAAAAGAGTTGCATTGCTTGGCTAATTGTAATTGCTAATTTGGTTTCTTCCTGACTTAGTTGATATGGTTCACCAACCGCTCCCTTATTTAAATTAGAGCGAAAATCACCCTCATTTTGAGTATGTAAGGCCCCAACTACCTGGTTATTGACCACAATTACCCTGACCGCTTCACTATTAGAAGTGGCAATGTATTGCTGCACAATGATCTGTTCAGTTAGCCCGCTTAAGGTTTGACGTAACTTCTGTTCATCTTTGCACAAAAAAACGTAATCACCAAAAGAACCATAGATACTTTTGACAATCACTGGAAACTGAAAACAGCGCTTAATTTGTTCGATGAATTGTTCCAACTTTTCCGGATATCAAGCTAAACGAAAGTTCTGTGGGCCAATTAATGTAGGAATCTGTTTAATGGTTGGGTGTTGCGCTAAAACAGCATGACTCAAGGCCTTATTGTCCGCGTTATTAATGGCAATCGAACTGTTAATAACGCGCAAACCAACTGATTCTAACCATTGCGCTAACGCTATGTTTTTATCCAAAAACAAAATGGTATTGGACTTAAAGGGTAGTTTGGTCTTATCCTGATTGGTATTAATATCAAAGCAAGTAAAGAAATCGAGTTGGATGTTTAAGCTCTGGGCTGCATCCTTTAACAGTTCCGTTTGGTTTAATTTGGAAGCAATGTTAGTAAAAGCAGGGTTATATACGGCGCTGATGTGCTTCATAAAGGACTCAAAGGTTTTTCATAATGGCTAACACAGTAAAGGGTCCTACCCCTCCAGGTGTGGGGCTTCACCATTTGGCAACTTTCTCTATCCCATTAGGATCGATGTCACCACACAGTTTGTTTAAGACATTACGTGATACCCCCACATCATAAAGAATAACACCTGGTTTAAAGTTAGTGGTCTTAAAGAAATGGGGCTTGCCAATGGCTGTGATCACCACGTCAGCGGTTTTAATTAGCTCCATGGTGTTGGGGGTATGCTTGTCACATCCCACCACCTGATGACCCGTTTCCTTTAAATAGTGAAAGATCGGCCCGCCACCAGTGACGCCTAAACCCACCACCACAATCTGCTTGTTGGTGAAATCATGGTGAAAGTGTTCTTTCAAAATTAACACCGCTTCCACCACACAGGACCTAATGGGTAGGGGGCAGTTACCAGTTAAGTAATCCACATCCTTGTAAGGATCAATTGCTTTTAAGAGCTTGTTTTGTTTTAGATGGGGATACACTGGCAGTTGCAAGATAATCCCTGTTACCTCAGGATCAGTGTTGAGTTGTTGAATTTTTTCTAACAATTCTTTCTCAGTGACACTTTCGTGGTACTTAATTAGTTCACTCTGGGCCCCTAGAGTATTACAAGCATTCATTTTTTGGCGAATAAAGGCATCAGATGACACATCATTATTAGTTTGCAGAATGACTAATTTGACCTGACTTCAATCCCTGTTTTTGTAAGTTAAAAGGATTTCGTGTGCTAGTTGCTTGCCATCAAAGGACATCTCTTAAGTTGTTGTTTTTTGTCAAGCTAAACAAAAAACGCCTAACTTAATCTTATATGACTAGTTAGACGTTTGGATCTTTAATGGTGGAGATGAAGGGTCTCAAACCCTCAACCTCCTGAGTGCAAATCAGGTGCTCTATCAGTTGAGCTACATCCCCATTATTGGTGGAAGTAAATGGACTTGAACCATCGACCTCACCCTTATCAGGGGTGTGCTCTAACCAACTGAGCTATACTTCCAGGCAAAATCTTCGTACAGGTTCGCTTCATAATTATATTAATTTAGCCAAAGCTAGTTTTTAAGCGGTCTTATAATTAAAGCAAGTAGTTTTTATGGGTCTTGTTTTAAAACAATTTAACAGGAAAATAAGAACAGCTTTAATTCTGGCGCCGCTTTTCACCTTTGCACAGATTATTATTGATCTTATTATTCCGTCCTTTTTGGCCAGTGCTATTGCCGTAGTGTTCTCAATTGTCACGCTTAAACAAAAAGAAGCATCAGGCGAGGGAGTTGCTGTTGATTTTATTGCTGAAAGTAAGTTAAGTTTCCAAAGTGTCCAGGAAGCGCAAATAGTGTTAGCGACCTCAGTGATCTTGTTAGCACTCTTTGGTTTAGTGTTTGGGTTAATTTCCATTTTTTGTGCCAGCATAGTAGCGGGGAATACCTCTTACTTTTTAAGAAGGAAGATTTTTCGCAAGATTATGCACATTACCGCACCTTCCCATGACCAATATGGCTCTTCTACGTTGTTGGTAAGGTTGACCAATGACGTTTACTTAATGGAAATCATGACCTTTGATTTTTTGCGGTTAATTGTTCGTGCACCTTTCTTGTTTATAGGGGGTCTAGCCTTTGCTATTGCCACTAACTCTGACATGTCGATTTCGCTAGCCATTACCTTTCCTTTGACCTTCTTAGTGATTGGGATTCTCAACAAGAAATCGACCCCGTTATTTAAGAACAACCAGAAATCGGTGGACCAAATTAACGAACGCGTGGAGGAAGATGTGTCGGGTTACAAGGTAGTGCAGTCGTTTAACTTAAAGGACACGGAATGTCTCAAGTTTAAGGCAGCTAATGCAAGGTGAACTAAGTCGAGTACGAATTCCTTGTTTGTCAATACACTGAACATTCCCTTTACCTTCTTCTTTTCCAGTATGACTATCATCATTGCGTTGCTTTTAGTCTTCCAACTAGATAATTCAGTCAGAGTGGATCCCCTACCAGATAATGCTGCCATTAGACCCAGCATCTTTGCTTTCTTCCAGTACAACTTCTATATTGTCTTGGGTTTAATCTTGACCTCGCTAACAATGGTCAACTTTACCCGTTCCCGCGTGGCCTTGGGTCGGATTAAGGATGTTTTGAATAAACCGGAAATCCAACAACATGTGGTTCCTGATCAAACAGTTCTAGCCCCTAGCTTGGAATTCAAAAACGTGGCCTTTGGGTTAGGTAACAAGGAAAACCGGGACTTCTTGCAAGACCTTAACTTTAAGTTTGAGGCCGGTAAGACTTACGGAATTGTGGGACCTACTGGTTCGGGTAAGTCTTTAATAGCTAATATTATTGGGGGTTTATATGAACCCAACCAAGGTGAAATCTTTGTTGGTGGTCAAAGTATTAAAACGATTGACAGTGACTATTTAGCCAAGATGATCGGGATTGTCTTTCAGCAAAACATTCTCTTTAAGGGCACGATTGCTTCCAACATTAAGATAGGCCTGGAAACCAGGGAAGACTGGAAGCACGAACCAGATAGCAAAAAGGATGCTGCCATGAAACGCGCTGCTGCCATTGCTTGTGCTGATACCTTCATCGAAAAGTTCAGCGATACCTATGACCATACGGTGGAACAGTTAGGCAAAAACCTCTCTGGTGGGCAAAAACAACGCGTCGCCATTGCTAGAACCGTTATTACTAAACCACAAATCTTAGTGTTGGATGACAGTATGAGTGCGCTGGACGCTTTAACCGAAAAGAAGGTCCGGGAAAACATTGCCAACGAACTTCCGGGCACCACTAAGATCATTATTAGTCAAAACATTAATTCGATTAAGTACGCGCACAAAATTATGGTGATTGATAACGGTCGGATTGCTGGGTTTGACAGTGATGCTAAGCTCATGCAGAGCTGTGACATCTATGTCAAGATGAAGCAAGCACAAAAAGACCAAGGAGGTGATTTCGATGCTGTCGCATAACCAAAAACCCAATTCCTGGAAGATTTTGTGACGTTTAATTAAGTCCGTGCAAGGTCGCACTTCCTCCAAGGTGCTGTATGTCATGGTCTGTGCCATCTTTGGTATTCTCACTGGGGTTACTAACTCGATTCTCTTGGCCCAGGGCCTGGGTTTTATTTTTCCTACCACTAATACCGAAACTGATGGTATCCAATCAGTGTACTTACTGGTATTTGCGCACAACTTACCGGTCATGGAGCGCTTGACGATTGTTTGTGTTACCGTGGTTGTGGCTTACATTCTCATCTTTAGTTTTAACGTAGCGCAGAACTATTTAGGTTTAAAGCTGTACCAAGAGATCTGTGCCTTGTTGCGCTGAAAGGCTTACCTCAAGATTCAAAGTATGTCCACTAGTTTCTTTGATACGCAGAACAACGGGGATTTAATGAGTCGCTTGACCAACGACGTTTACAACATTAACAACCTGTATGCCCAAGTGGGTGGACAGACAATTCAGAGTTTGTTTATCTTAATGACAACAGCGACGATTCTGTTTGTTTTAAGTCCAGTCATTGCCTTAATTTCGCTAACAGTCTTAATTGCTTTAATTGCCCTTTCCTTTCTCTTTTTAAAGAAGGCACGCGCTGCTTATGCAAAGGTGCAAAACAACTTAGGGGACATGTCGGGTTACATTGAAGAAGTGTTGTCGAACCATAAGGTAGTTCATGTCTTGAAACTGCAAGAGGTGATGATTGATAACTTTGACAAGTACAACCGCCCGATGGTTAATCCCACCATTAAGGCCAATACCTATGCGGTGTTTATTTACTCCTGGTTTGGTTTTATTTCCAATATTACTTACTTAGCTTCGATCTCAATTGCCACGGCCTTTAGTGTGAATAACATTCCCTCCTTTGGGGTGAGTGCGATTAACTATTCGTTTATGCTGTCTTACATTGCGGCCTTACGTCAAACAGCACTCCCTTTAAACCAGATCTTTAGCTTGTGAAACTTAATCCAGTTAGGGATAGTAAGTGGCGAGAGGGTCTTCAAGATCTTGGATCTAGAAAGTCCCCAAAAACAGGCCACCATTACTAAGTTACCCAACATTAAGGGCAACATCCGTTTTGAAAAGGTAGCTTTTGGTTACAGTGCGGACAAACCAATTTTAACGGGGATTGATTTCTCGGTTAAACACGGTGACATTGTGGCCATAGTCGGTCCTACTGGAGCGGGTAAGTCCACCATTATTAACCTGTTGATGAAGTTCTACAAACCGTTTGCTGGGAAGATCTACATGGACAACTTTGAGATTAGTGAGGTGAGTGAAACTGCTTGACGGGAAAAGATTTCGATTGTGCTCCAAGACCCCTTCTTGTTTAGTGGCACGATTAAGGAAAACATCCGCATGGGACGGCAAGATGCCACTGATGAGGAAATTATAGAGGCGTGCAAGGTGGCCAATGCTCATGACTTTATTATGCGTTTACCCCAAGGTTACAACACCTTTATTTCCAACAAAACTGATTATCTCTCGGTCGGTGAGCGCCAACTGTTAACTATTGCCCGCGCTGTTATTCGTAATGCCCCGGTGTTATTATTGGATGAAGCCACGAGCTCCATCGATGTACACTCCGAAAAATTGATCCAACAATCAATAGGCAGACTAATGAAGGATAAAACATCGTTTATAATATCCCATCGACTCTCAATCATCCGTAATGCGACGCTGATAATTGTGATTAATGATGGAAAAGTTTTGGAAATGGGTAACCATGAACAATTAATGCGGCAAAACGGTTTTTATGCTCGATTAAAGCGCTCTGCTGTGAAGTAAGATCCAACCATGACGATTGCTGAAATAAGGAAATTTGCACAAACCACGCAAAAGTTTGTCGAAGCGGAAAACCTGTTTGAGCATGGTAACGTCGTCTTGCCCAAAAAATACCTCAATAAGGCGCGGGGCATGGCTGAAGTATTGTACAACAGCCAGGTCATTAAAGTAAGTTTTACGGCTAAAGATGGTGAGCTAACGTGTAAGTGTAGTTGTTTGGCTAACGTTGACAACTGCGTGCACATTGTAGCTGTATTGCTCAAATACCACCAAATGCTAGTGGAGTCCAAACGCAGTTTTAACTTAGCTGAAGCCTTTCACTTGGACTGTGATCAGGCCGAAATGCTGATTGAAAACCTCAGTTTAGAGATTATTGCCGGGGGATGAAACTTTAAGCTGGGGTTCACGATTAACTTAGATAAGCACAATCCCCAACCGAGTGTCTTGCGCTTTTATTGTTGTGATGCGACCTTTGTGTACTTCCTTCACTTGGAAAACGACACTTTGCACTCAGTCGAATTGTCCAAGTTTAAACCAGAGGAACGCGCTACCTTACTCTTCTTTGATAAATTGTGCAAGCAGTTTACAGTGGGGTATGACCGCAACAGTTTGCTCTTTCCGTTAGCGGGTTTTTTAAAGGAGTTACAAGCCAACACGGAGCCCACCATCTTTGTCTTTAACGATGACAAAATTGACAACATCCTCTTTTTAAGGATTTCCAAAAAGCACCATGGTTTAAACCATGTTTGTGGTTTTAGTGGCAAGAAGGTATTTGACTTTGTTACTTATAAACAAAAGGAAAAGCAGATTGTGTTGCGCTCAGCGTACCTGAGTAAGTTCACTGACTTTAAGTTTCCTTACACCATTAACATCTACAAACTCCAGTTTGGGGAACCGCTGTTCTTTTACTTTTTAATTCAGCTAAAGCGTGATGGCTTTAAGAACTTCTACTTTCAAAGTGAAGACAGTATTGTCAAGGAAAAGGAATACCTACCGAAGCTGTACTTTAAGGTCGAGTACGATCCGGTCAAAAACAAGTTTGTCAGTGATGCCTTCTTTAAGTACAAGAACCACTTTAATAAGGGCACAACCACGGTGTATCCGCACCGCTATTACATGGCTAAAAAGTCGGATCGCGGTGGTTTTAACCGCTTGTTGTTTTACGAAGAGGCCGTGGAAAACTTCTACCAAGACCAGTTTGATTTGGGTTACTTCCGTAAGTTTGAACACCTACCGATCCAGGACAAAAACCAGATTGAGGCCTTTAAAGCAGCGCTAGATGACTTAATGCCCGTGGATTTGGCTGAAGTGAGTTTATCCGATAACTTACTCCACCAAAAACCGCTTCATTTTGCCCTGTCTGATCTAGAAGCAGTTGCTGTTGATGACAAACAAATTAAGTTAAGTTTTGCCCCTAGTGCGGTTGAACTGAAATTAATTAAACGGATCTTATCCGCTTACCATAAGGGCAATGTGGTCTGTATCGACCAGGAGAGTTGGTATGACCTCAAGCAACCAGCTGCTAAAGAACTAATCCAGTTCTGAAACCAGTTTGACTTACGCAACGCTACCAGTGATGGTAATCACATCTACCTCCCGAAATATTACTTGTTTGAAGTGGCCAAGATCTTTAGTCAGTACTTGGACATTAAAAACCTGTTTGATGTACCGACCATTAAAAAGATTGAAGACCAAAACAACAACGTCTTTGACCTTTCCTTAGAACACAAAAAGATTACTTCACTGCGCAACTACCAACAAGAAGGAGTGAAGTGAATTCGCGGTTTGGAGGAAAATAAGTTTGGTGGTATTCTGGCTGACGAAATGGGGTTAGGGAAAACGGTGCAGGTGATCTTTGCGCTGTTGGACAGCTATCTCAAAAACCACGTTAACCTCCCTAGCTTAATTATTGTTCCGGCTTCTTTATTGTTGAACTGAAAGAGTGAGTTTGAAAAGTTTGCCCCGCAAATTAAGGTCAAGGTAGCTAACATCCCTTCTAAGGAACGCGGTGAGTTGTACGAAAAACTTACTAACGAAATCCTGATTGTGAGCTTTAACGTGTTGCGCAGTGATGTGAAGTTAATTACCAAACAGCGCTTTCACTATGTTGTAATTGACGAAGCGCAGGGCATTAAAAACGACAGCTCTTCAATTACCAAAGCTGCCAAAAAGGTGAAGGGCAACTTCTGTTTGGCCTTAACTGGTACGCCAATAGAAAACCGCTTGTTGGACCTTTGATCCTGCTTTGATTTTGTGCTCCCAAGCTTTTTAGGGAACAAAAAGCAGTTTACCGACCAGTTTGAAAAGGAAAAAACCGACCAAAGCTTCCATTTGTTAATGCAGCGCACTAGTCCCTTTATTTTGCGTCGTACCAAGTCCAAGGTCTTGAAGGAACTGCCTAACAAAATTACCACCGATATTTACGTAGAATTAAACCCAATGCACCAGAAGCTGTATGAGGAAGAACGTGACCGCGGTTTGGAGGAAATTAAGCAAATCCAAGACAAGAGTTCCTTTAACATCTTGACTTTAATCTTGAAGTTAAGGCACTTGTGTAGTTTACCCAAAAACAGTCAGGGTATTTTGGAAAACAGTGCCAAAAAGGAAGCTGCTTTAGAAATTATCCACGAAGCGATTGAAAACCAGCGCAAGATTATCCTCTTTACCCAGTTCATTGATGTAATTGACCACTTTAAAGACACCTTTAAAGAGCAGGGCATTGAGTACTTTATCTTTGATGGCCGTAAGTCTCCTAAAAGCCGTCACAGTATTATCGAGAAGTTCAACAATGCCAAAAACCCTTGTGTGTTGTTAGCGTCCTTGAAAGCAGGGGGTGTGGGAATTAACCTCACTGCCGCTGAAGTGGTAATCCACTTTGATGTGTGGTGAAATACCGCCGTGGAAAACCAAGCGACTGACCGGGCCCACCGCATAGGACAAAAGAAAACAGTCCAAGTTTACCGCATTATTGCTAAAAATACGATTGAAGAGCGGGTCTGTCAAGTGCAGGCCGAAAAACAGGAGTTAGTGAGCAAAACGCTAGTGGAAGACGTCAATTTTTTCGAATCCTTGACCAATGAGGAATTATTACGTCTTTTTGAATAAATCAAAGCAGTTTTATAATTAACACTCGAATGATTAAAGTGATAAATGGCCGCTGGTAAACGTGATTATTATGAAGTGTTAGGCGTTTCCAGATCCGCCACCGCACAAGACATCAAACGAGCTTTCCGCAAGCTTGCTATGCAGTACCACCCCGACCGCCATAAGGGTGAGGGTGAGACTGTCCAAAAACAAAACGAAGAGAAGTTTAAAGAGGTCAATGAGGCGTACGAGGTCTTAAGTGACACCGAGAAGCGTGGTATGTACGACCGCTTTGGCCATGAAGGGTTAAATGCCTCTGGCTTCCATGAAACTGGGTTTAATCCCTTTGACATCTTTAACAGTGTCTTTGGTGAGGGCTTTTCGTTTGACATGGACGGGGGTTCACCGTTTGACTTTATCTTTAGTAGGGGCAAAAAGTCGCGGCAAAACCGGGTTGTTTTGCCCTATGATTTAGAGATTGCGGTGGGGGTAGACATTAGCTTCTTTGAGATGACTAATGGTTGTACTCGGACGATTGAATATACCAAACGGGTGACCTGTAGTGCCTGTGATGGTTTTGGGGCTGAAGGGGGAGAAACCGGGATGGTTAGCTGTAACAGTTGTGAAGGCAATGGTTTTATTTTGAAGAATCAACGCTCCATCTTTGGTACAGTCCAGTCCCAGATGTTGTGTCAGAGCTGTGGTGGTCAGGGTAAACAGGCCAAACACAAGTGTAAGACTTGTAAGGGAAGTAAGTACAAGAAGGTACCAACTACCAAGGAAATTCAGATTCCCGCTGGCATTTACCATGGTGATGCGTTAGTCGATGACCATGGTGGTAATGAATTCGGGGGTCACGTTGGCAAGTTAGTGGTCCATGTGGGGGTACTACCGAGTAAGATCTTTAAACGGGTTGACCATAATGTAGTAGCCAATGTTTTAGTCGATCCGATGATTGCTATCACCGGAGGGAAAATTGAACTCCCTACCTTAGAAGGCATTAAGGAATTCAACTTACGTGCTGGTACTAAGAGTGGCGAGCAAATAGTAATCCCTGGTGGTGGGATTAAATTTACGAAGAACTTCCGCAAGAAGGCCGGGGATCTCATTATTATTATCAGTTATGCCCGTCCAAGTGAATACAGTGCGCCAGAGTTGCGCAAGCTCAAGGAATTTATTAAACCTAATCAAGAGGTAAAACAATATTTAAACGCACTTAAGAATGAATACAAGTCCTAAGCAAAGTGGTTATTTAAAGGTTGGTAATGGTCATGAGGTCTACTTTTGAACTGCTGGTAATCCCCAAGGAAAATCAGCACTTTACGTTCATGGGGGACCAGGTTCGGGTACGGATGCGGGTTGTTTAAAGTACTTTGACTTAGACACTACCTATGTAATCTTGTTAGACCAACGGGGTTGTGGTCAAAGTAAAGCGGTTAATCCCTTACTGCACAACACTACCCAAGACTTAGTTGGGGACTTGGAAGCGTTGCGCCAACACTTAAAACTTGAGCGTTGGACCCTATTTGGTGGTAGTTGGGGTTCTACTTTAGCCTTAGTGTATGCCATTACCCATCCCCAAGTAGTCGAACAAGTCTTTTTAAGAGCGCTCTTTTTGGGCCGTGAACAAGACTGGGCTGAAATGCTGTTGGGCTTGGGCAAGCTCTTCTACCCTTACGAACACCAAACGTTACTGAAAGCCATTCCCCAGGCTTGTCGCACTGACTTTACGAAATTCACTAACTACTTTTATGAAGTGTTGCAAGGTAACGATTCCGCTTTAAAAACGCAGTTAGCCAACGCTTGGGTTAAGTGGGAAAATACCTTACTATCACCGATTAGCTATGTCAAAGATGAGAAAGCAGAAGATGCGAACTTTACCTTTAAGTTAGCGCTCTTGGAATGTCACTACGCAAAGCACCACAGCTTTTTAAAACCGAACTTTATCCTGGAAAATGTTGCGGTATTAAAGGACAAACCGGTGCACTTAATCCACGGCCGTTTTGATCTAGTCTGTCCGTTAAGTCAAGCCTTGGAACTCAAACGTGCTTTACCAACGCTTAACTTGTATGTAACTAACAATGCCGGGCACAGTGGCAGTGATCCCAATAACCTCACTACCATTAAACACCTTTTAAAAACACAACTGTAATGAAACGGTGTTACATTACCACCCCGATTTACTACGCCTCGGGTAAGCCCCACATTGGCCATGCCTTTACGACCATCTTAGCGGATGTGATTAAGCGGTATAAACAGCAAAACGGTTATGAAGCTTACTTTCTAACTGGTACGGATGAACACGGCAACAAAATTGAAAGTAAGGCCAAAAGTTTAGGTCTAGATCCCCAAACCTTTGTGGATCAAAACGTGGCTTACTTTCAACAAATGTGAAAGCAGTTAGACATTAACTTTGACCACTTTATCCGCACCACTGATCTTAGTCATAAAGCCCAAGTTCAACATGCCTTTCAACTACTTTATGACAAGGGGTTAATTTACCAAAGTAACTGGGAGGGTGCTTACTGTGTCGAGTGTGAGCAGAACTATTTCACTTATGATAAACAAACAATGTTGTGTGAAATAGGTCACCAGTTAACCCTAGTCCAAGAACCATCTTTATTTATTGCCTTTAAAGATAGTAAGGACTGGATTGGGGAAATGATTGCGACAAACAAGCTCAACATTACCCCTGAATCCAGAGCCGCTGAGTTAAAGAACAACTTCTTGGATGGGGGTTTAAATGATTTAGCTTTAACACGGCAAAACGTGACTTGGGGAATTCCCGTACCCTTTGACAACAAGCAAACGATTTACGTGTGGTTTGATGCCTTGTTTAACTACATTACTAACTTAGGGTTTGCCCATAACGACCCTAAATTTAACAAATGGTGGAACAATAATGATGAGGAACACGAAGTCATTCATTTAATTTCACGCGAAATTACCCGTTTCCACTGCATTTACTGACCAATCTTTCTCCACCAGTTGGGTTTTAAACTACCGACCCAGTTTCTCTCCCATGGTTGGATTGTCGATGGTAATGGGCACAAGATGTCCAAGTCGTTGGGCAATGTCATTTCCCCCGAAGAACTATTGGCCCAGTTTGGTGTTGATGGTACGCGTTATTGCTTACTTAAAGAAATGCGCTTGGACAAGGACAACCGCTGTAGTATGGCGATCTTTAAGGACATTTACAACGCTGATTTGGCCAACAGTTTTGGTAACCACGCATCACGTACCTTTGGCATGATCAAAAAGTACTTGGGCGGCCAGTTAGACTTTATCGAAGTCCAAGACCCTCAAGTAAAGCAGCTAATGGACCAAGCGAACCAAGCGATGGTTCAGTTTGACACCGCTTGAAATAACTTCCAGTTTTACAAGGGCATTAATGGCTTACTGCAACTAGTCTTTCAAGCTAGCAAGTTAATTGACCAGTTAAAACCGTGGGAGTTAGTCAAACAAACAGATTACACCTTACTCAAGCAATTACTGTTTGCTTGTGTCCGTTGTACCCAAGTGTGCTTTGTCTTGTTAGCGCCGATCTTAGTTCATACCAGTACCCAGATATTTGACCTGTTTAATTTCAGTGCCCAAGCACGTAGTAAGACCCACCTAGCTGATCCACAACAACTGCAAAAAATCAGCTTAGCACCTGTAATTCAACCTTTATTTAAAAGATTGGATTAATTAAATTAGATTAGAATTTAATGACTATGCAAGTAGAATACCTCGATCTCATCTCCCAAGCGAAGGAGATTGCTGAGACCCAATTTAAGGCGGAACCGTTTTCCTTTGATGCTATTTGAAAGGAAGTAGTGAAGCACTTTAAGATCTCCAAACAAGACGAACCTAACCTAATTTCGCGGTTTTACCAAGACTTTTTGGAAGACCCTAACTTTGTTTATTTAGGGGAACGCAACTGGAAGTTAAGGGACTTCATGAAGTTTGATGAATGGAACAAGATTTCCCAAGCAATGTTTGTGACCAAGGAAATCTTTGAAGAGGGTTATGAAGACCTGTCCAACAAGAAGAAGGAAAACGAGGAAGAAGTGAATGACTTCATTATGGGCAATGATGGTGATGACAACAGTACCGGTGATGAAATTGTGCAAGGACTCATTAACGATTTTCGCGACGATAACCAATAATGCTAGTAAACATCAAACAAATGTTGCAACACGCTAAGCAACACCACTATGCTGTACCCCACATTAATATCAATAACTATGAATGGGCTAAGGCAGTATTAACAGCGGCTCAGCAGGCCAAGAGTCCGATTATTGTCTCCACTTCTGAGGGCGCTTTAAAGTATATTTCCGGACACCAAGTGGTAGTACCGATGGTAAAGGGCTTAGTCGATGCTTTAAAGATTACTGTACCCGTAGCACTCCACTTAGACCATGGTAGTTATGAAGGTTGTAAGGCAGCGTTGCAAGCTGGGTTTAGTTCCATTATGTTTGATGGCTCGCATTTACCCTTTCAAGAAAACTTCACTAAATCCAAGGAGTTAATTGAACTAGCTAAACAAACCAATGCTTCTGTGGAACTAGAAGTGGGTACCTTAGGTGGGGAAGAAGATGGTATTGTGGGTCAAGGTGAATTGGCCAACATTGAAGAGTGCAAGCAAATTGCGACCTTAAAACCGGATGCCTTAGCTGCTGGGATTGGTAACATCCACGGGCTTTACCCCGACAACTGAAAGGGTTTAAACTATGAACTAATTGAAGCGATTGCTAAAGCGACTAACTTACCCTTAGTGCTCCATGGTGGTTCGGGAATTCCGGAAGCGGATGTCAAAAAAGCAATTGGCTTAGGCATTAGCAAGCTCAACATTAACACTGAATGTCAACTAGCGTTTGCTAAAGCGATTCGGGAATATGTGGAAGCCAAGAAGGACTTAGATACCCACAATAAGGGCTATGACCCACGGAAATTGTTAAAGTCACCTACCCAAGCAATTGTGGACTGCTGCTTGGAAAAGATGCAGCTCTGTGGTTCAACCAATAAGGCTTAGATGTTAAGTGCGGGAATTGTTGGTTTACCCAACGTTGGTAAATCAACCTTGTTTAGTGCTATTACCAACCTGCAAGTGGAAATAGCTAACTATCCCTTTGCCACGATTGAACCGAATGCCGGGATAGTTAACGTAATCGATGAGCGCTTAGACAAACTAGCGAGTTTAATTAAACCCGATAAGGTTACCCACACTACCTTTCGCTTTGTTGACATTGCTGGTTTAGTTAAGGGTGCCAGTAAGGGAGAAGGACTGGGCAACCAGTTCTTGGCCAACATCAGAGAAGTTGACCTTATTTGTCACGTAGTGCGTTGCTATGAGGACAAGAAGATTGTCCACGTTAATAACCAGGTCGATCCCGTATTTGACTTTGAAATTATTGTCAATGAGTTAATCCAGGCCGACATTGAGGTGGTGAATACCCGGATTGGCAAGATTAAGCGCAAGGCCGAATCTGGTGATAAGCAGTCCAAGGAAGAATATCAACTGCTAGCACCCGTTTTACAAGGTTTGCAACAAAACCAGATGGTGTTGCATCTAGTGAATGAAGTAGATTTAAAGAAACTCAAGTCACTTAATTTACTAACCGCCAAACCGATCCTAGTGGTGGCCAATGTGTCAGAAGCGGATTTGTCCAACCTTGACCATAATCCCCACCTCACTCAGCTCAACCAGTTCTTAAAACAACATAACTTACCCCATGCCATTCCTGTTTGTGCATTATTAGAAAATGAGTTGAGTAGCTTGGATGCCAATGGTCGTCAAGACTGGCTCAAGGAGTTGGGTTTAAGTGATTACCAAGGTTTAAACCAACTAATTAAAACGGCTTATGATGCGATTGGTCTGTGGTCATTCTTTACCTTTGGTAAGCAAGAAGTGCGCGCTTGGGCTTTTAAAAAGGGTTGGTTAGCACCGCAGTGTGCGGGTGAAATTCACACTGACTTTGAACGTGGTTTTATTAAAGTAGAAGTAATTAGTTGAAACCAACTATACGAATTAAAATCGCTCCAGGAAGCCAAAAAGCAGGGCCTGGTCCGTTTAGAGGGCAAGGCTTACGTGATGCAAGATGGTGATGTGTGTCACTTTAAGTTTAACGTTTAGCAAGTAACTGCTGAAACGAGTAAACAACCCAACTTTGCTTGTGGTTGTAACTAATACATAAACAACACAACTCATAGTTAAAAAAGACTTCCACCTGTCCCTTAGTTAACTTGTGGGTTTTAAATCAAGCTAACAGCGCTTTTTGACTGTCGGCATAGGCATCAAAACCAATGTTAATTAGGTTAACGGTGAGGTTCTTAGTTGGTTTTAACATTTGGTTAACCTTTCACCCTGACCCTCAACCAAGATAGGGTTTGGTTTCTATGACTGACTCCCCTTTAAAGTCCTTGGTGCCAAAGTACACCTTCGGGGCATTGGGCTCAGCTTTCTTAAAGTAAAACAGGGAAGGAAAACCCAGTTTGTTAGCGAGGTGCCGTGACCTTTGGTTGGATTCATAAAACGATCACACTAATTCCCGCGGTTGCAACGTACGCTGGTAGTACTTAAGAAAGAAACTAATTGTTTCTAAAGCGACACCACTTTGTTGTTGACTGGGATCAATCCAAAACGATAATACCGGCATGTTGCCCCATTGCCCGACACAAAACCAACCCAAAAACTGCTTGTTTTTAAATAAGAGCGCTAACAGTTTACCGGGTTGCAGTTTGTCGTGGATGTATTCCAAGAACTGCTCAAAGTTTTCCACAATTTGCGCCATATAGTTAGTAGCACTTAAGGTGGTCCGGTGCTTCATGAAAACATTAAAAGCAGCTTTGACCTGTTTATTTTTGACCAACTTATTGGGGTTTTTCAGTGCCATTAGTTGGTAACGATCACCAAAAAAGCAAAACTCAAGAATAGTGTTTTTATCCATTTCAAAAGTCTTTTATAATCTTAAAGATAGTTGTGCAATTCAAGTATTTATTCACCGTGATTATTCCCACTTACAATTGTGGTCAATACATTCCAAAAGCATTAGATTCCTTGCTTTTACAAGGTGAATATTTTACTAAGACCCAAGTTTTAATCGTCAATGATGGTTCGACTGATAATACCAAGCAGATCGTAGAACCCTATACCCAACAATACAGTAACATTGAGTACTTGGAAAAACCCAATGGCAATTGAGGCAGTGTGGTCAACTTTGTCAAACAAAACCAGTTAGCTAAGGGTCAGTACATTACTGTATTAGACAGCGATGATTATTTTCTAGCTAACGCTTTTCAACGCGTAGCTGCTCACTTTGGCCATGACATGATTGTGAGCGCCTTTTACTGTTACATTAGCCCTAAAAGAAGGCGTTTTCTCAAACCATACTTTGGTAAAACGGGGGTAATTGAACAAAAGACTAAACTACGCACACCCCATTCCCAACCCTTGGCTAAGTTCTACCGCCACGAGATCTTTCACTTGCTAGATCCTTTAAAAGAAAAGCTGTTTTACCAGGACTGTCTGTTGTACCATAATGCCATCAATAAGGTGCAAAGTGTCTTTTACATTTGTGAACCCTTAGCAGTGTGGTATGCCACTAGACCAGGTAATTCCACCACTATGCCCTGAAATAATGCCGATAAGTTCCAGGCCTGGTGTGACTTGCTCAAACAAATGAACTTATACGGCGCGGGTATAGTGATTTACATTTACACTATGTTACCTGGTTTTTTAAAAGAATTAAAACGGCAACAACTAGTGTTAGATTTAGCTAAAAAACCCGCTTATACCTGATTGCCCCAACCCTTAGCATTTCTGTTTGGTGGCTTAATGGCACTCAGGACAAGAAAGTACATTCGTTACCCTAAGAATTAAGATTAAGATAGATAAAGCATATGGCAGACATGATTGAAGCGAAAAGTTTACGCAGTGGACAAACGATTTTTGGTCCCAATAAAGAGATTCTGTTGGTGTTAGAAAACACCTTTAATAAGACCGCAATGCGCCAGGGAATTGTTAAAACCAAGGTCAAAAACTTACGCACTGGGGCCATTGTTTGGATTGAGTTTACCGGAGACAAGTTAGAACAAGTCATTATTGACAAGAAGAAAATGACCTTCTTGTACAAGGATGGTGCTAACTATGTTTTTATGGACCAACAAGACTACAGTCAAATTGAGATCCCCGAAAAGCAGTTAGAGTGGGAAAAGAACTTTATCACTGAAGACAGTGAAGTTACTATTATTAGCTACCAAAGTGAAATTTTGGGCGTTAACCTACCCGAATTAGTGCCGATTGAAGTGGAATTTGCCGAAGAAGCAGTGCAAGGTAATACCGCTAACATGGCGCGTAAACGGGCTCGGTTAGTTTCGGGTTACGAATTAGATGTGCCCCAGTTCATTCGTACTGGTGATAAGATTGTGATTTCCACCATTGACGGGAGCTATCGTGAACGGTACAACAAGTAAATTAACTAGAACCCAAAGACGGATTGCAATTGTGGAGTTTATCTTCGCCACGCTGTTCTTTTTACCGAAAACTGCTGACCAAATCCAAGCGGCCTTTTTGGACTATGATGTGCCAGAACGTCCGCTCAACGATTGGCAAAAAGAGATTGTCAAAGTCTTTAGTGAACGTTGTGTGGAATTCATTGAACTAATTGAAAACCAACAACAACGCAACCAAGCAGAAGTACAAAGCAAGTACAACAAGGTTTCCGGTAAAAAAGTCGACTTATTGACTAAGGCCGTGATTCTCTGTGCTTTGAGCGAACAACACGCGCAAGCGACTGATAAACCATTATTAATTAGTGAAGCCTTACTGATTATGGACCACTATTCCCAGGTACCCGAAAAGAAGCAAACCCATGCTTTATTGGATAAACTCTTGTAATGCGACGTAATTGAAGGGAACACTATAACGTGTTTGTAGCAAACCTAGCCTTAGTGTTGGGTTTTATGTTGAACATTGTGGTGGCGCGGTATACCTTAACTGGTGCTACGCCCCAAGCACGTTTTTTATTCCTCACACCCTTTTTAGGCATAGTAGCAGCGAGTATCTTCTACTTCTTTGATGTGAAGTGGTTCTTAGCGGATTATCCCTACAAGAAGTTCCATTTTCAAAAGAAGTGAACCTGGACTTACTTAAGTGGCGTATTTGTTTTCTTTGCTAATATCTTGGTTAATGTGATCTTGTTAGCACTCCTCGTTAACCAGATGACCAACCAAATCCTTAGTGAAAAGTATACGGGTTTACTGGACAACGCTTACCCACTACTTTGATCCGCTGTGGGTGTTAGTATCTTTTTGAGCTTAATTAGTATTGGTTTGAGCAAAACAGCCCACTTTAAAATTGATGTGGAAATGCTCAAAGCGAAAAAAGGCGAACCCACCGCCGCTGATAAAACCGACAGCCGGCCGGTAGTTGTTGATTTAGACCAAACTAAATCAAAAAAAGATGGGGATAACCCACCCCAAGCTTCGGGTGATATGACTAGTCTTTAGCTTCGTTACCGAACAAGCGGTGGTTAATTAACTCGTTTAAACTAGTGTCATCAAACAAGGTGCAGACCGCAGTAGTTGCAAATGCCAAAGCACAACCAGCACTACGACCAGTGACGAGTTTATTGTGGACCGTAACTGGGCTAACATTGCGGTGCTTTGATCACTTTAAGCTATGATTGGGAAACGAGGAGTAGTTTTCCGTTTCACTAATAATGCCGTGCTTTTACAACACGTTGGGGGTATCACAAATGGCAAAGACATACTTGTCATGATCCTTAAAGTAACGGATAGTGTCGAGTAACTGTTGGTCATGGTCTAATACCTTGGTAGCTCCCATACCGCCAGGGATAAAGACACCATCAAATTCCTCCAGATTAACCTTGCTAGTAATCTGGTTTACTAAAGTTACCCCATTACTGCCCTTAACTGTACTTAAGTTAGGGTGATAAATCACGGTTTGTAATTCTTTAACAAAACCAAATACCACCATTGTGTTGGTGTATTCCACATCATTCATGTCAGGGTAAACAATGACTAAGAGTTTTTTCATCTTTTATTTAGTACCAAATAAGCGATCCCCAGCATCACCAAGCCCGGGAATAATGTAATTATCCTTATTTAATTTTTCATCAATGGCTGCTAGGTAAATGTCCACATCAGGGTGCGCTGCTTCCACGGCTTTAATGCCCTCTGGTGCTGCTACTATGGCCACCACACTAATCTTAATCGGGTGGTTCTTTTTAATGGAACTAATGGCTGTGGCTAGGGTAGTACCAGTAGCTAGCATTGGGTCTAAGATAATCACGTGGGAGTTGCCAATGTTTTCGGGCATTTTCTTATAGTAAGAAATTACATTAGTGCCCTCATGTTCACGGTAGATCCCTAAGTGACCCACACGAATCTTTTCCGAATAGCGCACAATTGGGTCAACCATACCCAGGCCAGCGCGCATTATGGGGACTAGAACAATTTCGTTTTTGAGCTTATAACCCTTCGTTTTGGTAAACGGGGTTTTTACAACAATTTCTTCTAAGGGTGATTCCTTAGTTACTTCAAAAAAGAGTAGTGAAGTGATTTGGCTTAACGCCATCCGAAATTGCGAGGAGGAAGAAGATTTATCACGTAGCTTTGTCAATTCGTTTTCGATCAAAGCGTGGTGCACTTTTTTGATCACGGTTAAAGATAGTCTTAATAAGGTTATATTAAAGATTGACTAAAAAAGTTTTTTCTGGTTGTCTTGCTCCCTAAACTCATCGAGTAGTTGCATTTGGGTAAACGCTTCAATGTGTTTTTTGGTAATTTTAGTGCGTTTTTTAAAGTCATCGAGCGAACTAAAGGGCTTTTCGTTACGCGCTTCTATGATCGAATTGGCCACAGCTTCCCCCATCCCCGGAATCGTAATAAAAGGGGCAATTAGCATGCCGTTGTGCTCGACAAAACGAGTGGCGTGGGAGTGGGTTAAAGAGATTTGTTCTAGTTTAATGCCGCGGGCCATCATCTCTAAATAAACCTCGTAACTGGTTAACAGTTCTGCTTCTTTGGGTTTAATCCGTTTAATGCGGTACAGTGTGTTGAGCTCCTCTAGTTTTTGCTTGACAAAGCTCACACCTGATTTAAAACCACTGACATCATGTTCCTTGAGTTTAAAGCTCAACAAACAAGCATAGTAGCTCAACGGGTGGTACAGTTTGAACCAGGCAATTCGTCAGGCCATTAAAACATAAGCCGCGGCGTGGGCTTTCGGGAACAGGTAGCTAATCTTTAAACACGAATTAATCCAGTGTTGTTCGACCCCATTGCTCTGCATTAACTGCACTTCCTTGGCATTGAGAGCAATTCCCTTACGCACCTTTTCCATGATCTCAAAAGCATCTTTGGCCTGCATCCCCTTATGAATGAGGTAGAGCATAATGTCATCGCGACAGGCAATTACATCCTTTAAGCTTAAGTTTTGGTCCTTCAAGATTTTCTGCGCATTGTCCGCCCACACGTTCTTCCCGTGACTTAGACCCGAGACGCGAATGAGGTCACCAAAGCCCTTTGGTTTGGTTTGTTCCAAGATCTTGCGAACGAACTTAGTACCAAACTCGGGAATCCCAATCGCTCCCGTGGGTTCATCTAAGTAGTGCGGTTTTAGTTTTAAGGGATTGACCGACCAAAACATTTCGGTGAGCTTCTTGTCAAACCGGGGAATGTTTTGTGGATTGACATGGGTAAGATCGGCCAGGTGCTTTAACATAGTCGGATCATCCTGGCCCAGAATATCTAACTTTAAGATGGTGTCCCCTAACGCATCATATTCAAAGTGGGTCGTTTTTCAATCACTGGAAGTATCATCAGCTGGATAACCACACGGGGTAAACTCATATACCGATTTGTGGTTGGGGAAGATCATAATCCCCCCGGGGTGTTGGCCGGTCGTGCGTTTAATCCCGACTAACTTTTGCTTAAAGCGTTCAATTTCGGGAGCGGTGGCCAATTCGGTTTGTTTAATAATTTCAAAGTAGTTGCGCGCATACCCGTAAGCCGTTTTTTCGGCCACTGTCGCAATTGTGCCAGCACGGAAGGTGTACTGTTCACCAAACAGTTCCCTCACATAAGCATGGGCTTTGGCTTGGTACTCACTGGAAAAGTTTAAGTCAATATCGGGAATCTTGTCCCCAGCAAAACCCATAAAGGTAGCAAAGGGAATGTTATGACCATCACCCTTAAAGGCTGCTTTGGCACCACACTTTGGACAATCCCTTACCATGAGGTCATACCCATCATCGACCTCTTCGTTGACCTCAAAGTACTGACAGCTCTCACAGAGATAATGCGGCACTAAGGGATTAATTTCCGAGATACCAATCAGGTTAGCTACCAACGAAGAACCAATTGAACCCCGCGGTCCAACAAAGTAACCATCTTGAACCGATTGTTTGACTAGTAAATGGGAAATCCAGTAAATAATGCCAAAGCCATTGTCAATGATCGCTTTTAACTCTTCCTGGATCCGTTGTTCTATTAACTTAGGTAAGGGATCACCGTAAATGGCCTTGGCTTGACTCCAGGTTTTGGCTGCCAGGTTTTCGTTAGAACCTTCAATTACTGGTGGTTGGAGTTTGGTTTGCACTGGTACCAGGTCATCAGCGGTGAGTTGTTTCGCTAACTTATTGGTGTTTTCGACCACTAACTCGTAAGCTAATTGTTCGCCTAAAAAACTCATTTCATTGAGCATTTCCCCTGTGGTACGCACAAACACATTGGGAACCCGTTGTTCCCTTTCCTTATAGTTAAAGTGACGGTGTCAACGCCCACCCAGACCCTTGGCACAAACTATGGCCTTATAGTACTCATTCTCCCAGGGGTGAATAAAGTAAGCATCCGAGGCAACTGCCACTAACTTATTGAGTCTTTGGGCCGTGTCCACCACTAACTTAATTGCGTTAGGAATGGCTTCTTTTTTCAAACCTTCCCTGATGGTGTAACCAGCATAAGCACCAGGTGGGGCAATTAAAACAAAGTCAACTCTTTTAATGGCCGCTTCTAATTCTTTAAGCGGTTTAGTCAATGCCGCTTTAAAGATATCACCTTGCACCGGGTTATCAGTAATGACAAAGGATTTAGCAAACTTTTGGAGTGTTGAAGTTAACACTAGGGGTCGAGTAGCGTTGTTATCGGTGAGCGCAATTGATAACAGTTCATACAGTTTAGCAAACCCACTTTGGCTCTTAATGTAGATAATGCCGGTATTGGTAAAAGTGCGCTTGAGCAGTTCAAAGTGGCACTGCTGGTTTAACTCTTGGTCTAACTGCTCGAGATTAGTAATCCCCATGAGTTCGACCTGTTCCTTGAAGTAGTCAAAGACCTTTTTTAAGGCCTGGGTATCATAATCCGCTCGGTGCAATCGTTCATCATCAAAGTCAAACTTGAGCTTGGCACAAATATTGCTCAACGTATGGGAGGCAAACCCGGGATTTAAGGCCCAGGATAAGGCTAAGGTATCAATTAAGGGATTGGTTAGTGGCGCAATGTTGTATTTTTCAAACTGGGTTTGTAAAAAGGGCAAGTCAAAACTCAAACCGTTATGGGCTACCATTACACAACCATCTAGTCAAGCGCGCAGTTTTTCCAACCCCGCTTGCTGTTCCATCCCATCTTCTAGTTGTGCTTGGGTAATCTTGGTAATTTCGGTAACTGCTGCAGGTAAGGGTCCATCAATCTTGAGAAAGAACTGCATTCGTTCCACTTCACGGTTATGTTTGACCTTAATTCCCGCAAACTCAATCACCGAGTCATACCGACCGTGTAACCCAGTTGTTTCAATGTCGAAGATGACAAAGGTCGCATCCTTAAGTTTGGTTTTATCAGGATTGCTAACTATCTTAATGTGATCATCAATGAGGTTACATTCCAAACCATAAATGGCCTTCAAGCCGTACTTTTTGGCCACTTCATATAAAGTGGGATAAATGTGAATGTTGTCCTTGTCAGTGACAGCAATTGTCTTCCAATCGCGTTCTTTGGCAAACTGGGCGTATTCCTGGACACTGTTAATGCCATCAAAGGCACTCATCTTGGTGTGGAAAACTAATTCGACCCGTTTTTGTTTGTCCTGATCGGGGCGGCGATAGTAAGCGGGGGTTTCAATCGGGGAAATTTCCTTAATGATACCCTGGAGTAGTTGGGTCTTGGCATCACGTTCGACCTTAACCGTGGCCTTAACTCAGGTACCAATCTTAATGCCCTCAATTGTTTTGGGATCCTTGTAAAACCACTTCAAAATTAACGAACCACCTAGTTGGAAATCAGTGACATAAATATTAAGGGTATTACGGAGGTTGTTGTACCCAGGAAAGTCCTTTAACTCAAAGATTTGCCCCATTAGTTTGATCTCATCCATTTGTTGGTTAATCTTGTGAATGGGGACAAATTCCGTACTAAAGACCTCGGTTTCAAACTGCTTGTCGTACTTGGATACCTTCATTGATTTCACTTTTAGTTGTTTTTCCGCTGGTGGATTAAACAACGAGACAAAACCAAAGTGGGTAAACCCAGCTTGGTGCATCCACTTAATGAAGGCTTGGCGTTGTTCAATTAACCATTGGTCTAATTCTTGGGACTGGCAGTTAGCTTTCAGTTCATGGTTACTGTAGGACAAGAAGTTAGGATGGGTTAACTCTTGCTCTAACAGGATGCGGTACTTCTTGTCCTTACCAAAGAAGCTTTTGAAGTAGTCTTTGATAATGGCTAAGGTGACACTGGACTTAGTGGACCCGTCCTTAAAGAAGGTGGCATCTTCTTGGAAGGTAATCCTCAGTTCTGCCCCTTCCGCATCCTGAAAGCCCTCATACAGGGCGTTTCAAATGTCAATTGTTAACGGTTTTTTTAAAGTGAGCGCCACGAGAAACAGGGCGTTTTCGCGATCATTCTCGAGGTAAACCAGTTCAATCCGGTTGTTGAGTTGGTGCAATTCGTTGTGATCGAGAATATTTTTCTTAACTAAAAACTGTGATAATGCTCAAATGCGTTTACCCTTTTCGGTTTCGGTGTCGAATACCATATGTGCTAGTTCAAAGCTTATAAAACTGAAAAGGTAATAGCTTTATCTAACTGTTACCCGACGGTATTAGTTTACTATCTTGATCGTTTTGAAAACTTAAATGGTTAGTTAACTAGGTTCATTTCAACCACTACCTTTTTCAAGTTCCCGATTTGGTGTTCAAACCTAAAAAAAGGTTTGCTAAACTTAGGTGAGATTGCAAGATCTCGTTTAAATTTCCTCTGTTGCTAGTGGTAGTGTACCCAACTTTAATGCCATAAACGATTGCTATTTATTGATGAAAGCGTTCCGAAAATTGTTGCTGGGCCTCGCCTTACCCACAACCATCGGTCCCTTATTAGGCTTATTATTAACTAATACTGATACTGTCAAAAATGAAAGCTTAACTACTGTGCGCCACAGGTCCAGCATTAACCAGGACTTTGATGGCATTTTTCATACCGTTAAGTTATTAGAACCAATCCAGCAAAGTAATGCTGATCCCGCCGCTAGTTTTGCGCTCTTTCAGCAAAAGTTTCCCAACCTAAAACGTGTAGCCAACTCCACCTTAAATACCTTTGATGTCTACAACCTTTTGAGTGGTTGGAAGAGTAGCTTAACAGCGTACCTCAACCAGGTGTTGGCACTCCAACAACGGATTAAAGCAGCTGATCAAATTTTCCCTAACCAAAAGGAAACTTTACCCAAGGATGAGAATCCCAACATCTTTGAAGTGTTAGGTGCATATGGTGGTAAGGGTTTCTTTCCCACGCTAGGATCCAACGGGTTACACCTGCCACCACAGCTGTTCCAGTTCTTCCGTGACTTCCAACTAAGTAGCTTTACCATTAAAGACTTTGAGGTAGCGTTAGTGAGTGAACCGGACATAGTGCAACATGACAAAGTGCGCTATGCCTTTCAGGTGCAATTTAACTTAGTGTTACAATTACAAATTAACCGTAACCCAGTGTTATTTGACTTTAACCTACAGTTAAAGACGAATAACTTTGCCAATCAAACCAGTTTTGATGAATTGTTTAACGAAAAAACTAATCCACTTAACTGGCAGTTCTTTAGCAAGCTTAAAGTTAACCATTTAGTGTATGAAGGCAATGACATAACCCAGTTAGCTAATACCTTATTACAAAGCCAGTTTAATGTCTTACAACTTGATTTAAATAAGTCAATTTACCGCTTGAACTTAAACGCAATGGCACAGCGCTTTGAACACGACTGGGTACAACCGTTGTATGCCAAACGCACGCAGGCCAAAATTGCTTACGAAGCGGAACAAGCACGGATTGCGGCCGAGAACAAACGCAAGGAACTAGAACGGCAAAAGCTGTTAGCAGAATTGAAAGCCAAAGCTGAGCATTACCAAAAGATCAAGCAAGCACGGGAAAACATGCTAAAGGGCTTAAAGAGTATTACTGATTTTGTCAAGTTCTGAAAGAGTCCGGACCGTTTATTAGTTGGCTTTAACAAACAGGACGACATTACTACTCGTGCTGGGGTTTACAAGGCGTTACAAATTGCTTACGCTAACTATCCGCAGTGAACCTTTTATCTAACCCTACAAGGTTGGAAGAATGGTTCGGAGTTGTTGTTAAAAAGACCTAGTTGAACTAACTTATTAAGTGATATCCACTTCCAAAAGGCCTTTAACTTAAAGAACACCGTATCAGAACAAACCCTGGGTGCAGCTACCCTACCAGGTTATGGTTACTATGGCTTACGCATGAGTGACTGGTTGCGCTGGGCTTTGGGCTATTACTCCTATATCCATATGGGCGTACCGCAAAATGTACAGACCAAGTTTACGGGCACCCCAGATAATGAACAACAAGTCTGGATTGCTAACGAACCGTTTGAATGGAACAAACATTACGGTGTTGGTCCTAAATACAAGGATAAGGCCTACCGCTTTAACATGGAAATTAGTTTTGAACTAGAGGGTTGAATTGCTGTTAAGTGGTGAGCATGAGCCTTTAAGGGTTCAATTCCTGGGAACTGAAAAGGCAAACTCAAAGTAACCCATCTGTTTGATGGTATGGTACCAGTGTGGGAATTGGGCCCAGTCAATACCCATTTACCCCAATACAGCTTTACCGATCAGCAACAACTGTTGTTTGTACCCCACTCCATTCAAAAGATTGAAGCGATTGGTGCGGACAAAGGTATAAACGATCTTTTAAAAACCCAAAACCTCCATAACCTAGAGCGCTTGTCATACGAATCAACTAACCCAATTGACCTCATTTCTTACCTCTTGTACGCGATCCAGTACATTAAGGTTTAGTCACTTTCAGCAGTTAAAGTTCAGGTGTAAAGTTAACGATTAAGATCAAAAACCGTTCCTAAAAAAAGATCTTTTTCTAAAATCTAAGCGAGTTACAACTCAATTTAAATTTTCTCTCTGGTTGGTTCGCCAACAGTTTTAGCCACTTCAACATTCGTCAAACACCATTAAATGAAAACACTGCACAAGTTGCTGTTGGGGCTTTTCTTACCAGCAATCCTCGGTCCGTTGTTTGGTCTACTAGTTACAAAAACTGATGCTGAATCACCAGTTGCAGCATTACATAAATCGAAATCTTTTAGCAAAAACTTAGATAGTTTCCGCGCCTTATTTGTGGGCGAGGATATTGAGGAACAACTAGCTAAGGTACAACAACAAACACCAGAAGTTAGCTTTGCTAGCTTTCAACAAAAGTTTCCTGATAAGGCCAGTTTAAGAAACGGTTTTCAACCAATTGATGTTTACAACTTTTTAAGTGGTTGAAAAGGTGCTTTAGAAAACTTCTTGGCTAAGGTAGTTGAACTGCAAAAGAAGATTAAAGCAGCTGACGATATTTTCCCGAACCAAAAGAAAAATCCCGACAAGAAAGATAACCCAAATGTTTTAGAAGTGTTAGGTGAATACGGTGGAGATGGTTTCTTTCCTACATTAGGTAATAACGGTTTAAACATCCCGGACCAAGTTTTCCAATCCTTTAATAACTTCCAAATTGAGAACTATAAGATTAGTGACTTTAAGGTGGACATTGCCAGTGAACGTGACATTGTCCAACACGACAAGTTCCGTTTTAGTTATGTCGTTAACATTGGTTTAGAGTTAGCACTTTTAGTCAATAAGCGTCCGGTTTACTTTAACTTTAGCTTGGATCTGCGGACCAATAACTTCTCCAACCAAGCCGGTTTTAACGAAATCTTTAATGCGCCTGGTAACGCAGCTACTAACTGACAGTTCTTCAGCAAGGTTAAAGTGAAACAGTTGAACTATGATGGTAATGACAGTACCCATTTAGCCAATACCCTCTTACAAGATCAGTTCAATACCTTAAACTTAAACTTGCAAAAGTCGATCTATGAGTTGCCATTAACCGAAATGGAAACTAAGTTCCAGCAAGAATATGTGGAACTTTTACTAGCTAAACGTAGAGAGGAAAAACGACTTTGGGATGAAGAACAAAAACGAATTGAAGCAGAACGGAAACAAAAAGAAGCAGAGCTAGCACGAATTCAGCGTGAACTTAAAGAAAAGGCTGAGAAGGATGCGGCGGTTAAACAAGCTCAAACTAACTTAAAAACTGCTCTAAGTAGTGTGGAAGGCTTTGTGAAATTCTGAACTGAAGGTGAAGACCGCATTAAGTTGGGTTTCACGAAGGAGGACAACCTTTATACCCGTGCAGGTTTAGTGAAAGCACTCAAAATTTCGTTTGCTAACTACCGCGCTTGAACCTTCTACATTACCTTGCTTGGTTGAAAAGCAGGTAGTGAAAAGTTACTTCGGAAGACTAGCTGAACTAACCTATTAAGTGACGTTAACTTCCAAAATGCTTTTGGCTTAAAAAATACAGCATCGGAAGAACAAGTTGGTAAAACATCATTACCAGGTTATGGTTACTATGGAATTCGCATGAGTAACTGACTCCGTTGGGCATTAGGTTACTATGCTAATACCCATGTGGGTAGTCCACAAAATGTGAAAGCCACCATTGATGGTAATCCAAGTGATACCACCAAAGTATGGATTGCACCAGAAGACTTTAAACAGGGTGTACACATTGGTGTGGGGGAACACTTCAAAGGTAAGGCTTACAAGTTTAAGTTTGAAGTTAGTTTTGAATTAGAAGGCTCGATTGCAGCCCACTGGTGAACTTTAGCCTTACGTGGTAACATCCCGGGCTACTGAAAGGGTAAACTCCAAGTAACCCACACTTTTGATGGTGATGTACCTTCCTGGTACTACGGTTCGGTACATACCCACGCTCCTGAATACCGTTTTACTGAAGATAACAAGCTATTATTTGTACCGCACTCCATTCAAAAGATTACCGCTGTTGGTGGTGATAGTAATGGTGTCAACGGCTTGTTGAAATCACAAAACTTACATAATCTCGAACGTCAGTCTTATGAGGTTACAGCACCAATTGATCTAGTTTCCTATTTGATGTTTGCGATTGCTAAAGAACCAACAAATAACTGGTAGTTCTTTGCTGTATATAATTAGCGCTTTATCTAGCACTATTTAGATTTCAAAAAAAGTCTAAGTGTGAATAAAAATACTAACAGCAAAACCTAATCAATATAGGCACTGTTGGTATTTTTATTGTTATTTGGAATTATTTAGATTCCAAATTGTCTAGACGTCTGTTTATGCCTTCAAGTGCTTTCAAAATTAATCTAAGTGTTTCTCCTTGATTTTTTTGTTCAACTTGAAGCTCTTTAATTTGTTGGCCTTGAACGATAACAACTTCAGTTAGTCGATCAACTTTGTCCTCAACAACTTGAATCCTTTGAACCTGACTCTTTAACTCAGTTTCAATCTTTGTAAGTCGTTGGTCTTTTGATTTTTTGTACTCGTTAAACTCTTTACGCGTTACGTATTCAGTGTTCACTGGTATAACCGGTTTGTTAAGTTTAAATTGTCTGTTGAAATTGCCAGAGAATTCCACACTGTTGTTGTCTTTGTCGATAATATATCAACCTGAAAAAGTACCCTTTTGGTCTTTTTCACCATGTCATGAAATTCTTTTTCGTTATAAAACGGAATTTTCTCTTTCATTAAGCCATACCTCCTTTCTTTTCAGTGAAATTTAAAAAGTAACTACTAATTGCAACTTTGTTGTCAACGAGGATCAACTGCTTGATTGATCAATATGCAAAATTATTTCTGTGCATAATATTGTCTCCACTAAATAAATTGACCAGACTTTAAAAAGGTGCCAAAAATTAAATCAAAAAGTTTTTAAGGATTTTTAAAGGAGTAAAAGTACCAGCAGCTAACCAATAAAGATTAACTGCTGGTACTTTTACTTTGTGCTTTATCTACTTAGATTCCAATTTGTCTAAACGTTTGTTTATACCCTGGAGAGCTTCGAGTATTAATTGAAGTGTCTTGCCTTGAGCCTTTTGCTCAATTTGAAGTTCTTTGATTTGCTTGCCCTGAGCTCTGAATTCTTCCTTAGTTACAAATTTTTCACTAAGTTCAACTAGAAGATCTTGTTTGAACACTTCTAGTTTTTTATCCAGTTGTTTATGGGTGACGTATCCACTTTTACCTTTTTCCATATGGTTTAAATTGCCATGAAATCCCATGTCGTAGCTACCATCAGGTAGTTCTTCTTCCCATGTATAACAATCATTAATCTTATCTTTATTTAGTCTTTTCTTAAACAAAGTAAGGGAAATTTTGCGTTTAATGGCAAAGACCTCCTTTATTTATTGATTGGAAAAAGGTGAAAGTGTTTAAAGAGGTAGGCACCAACAGGTCACTGAGCGAGTTGACTGCCCAAATAAAATAAATGATTTTTTAAAACTGTCATTTTATTACGCATACATCATTTAAATTACCCAAAAAACAAAAAGACGCCAAAAATTAGTGTGCAAATTTTACGAGTTTAAAAGTGTCTTTTTTAACTGGTAGAACTTTGTCTCAAAAATTAACAACAGTTAAACCGGCAAAAATAAAAATTTTGGGTTAAACTTTGGGGCGAGGTTTCCTCAGTTAAATATCCTTTTGGTTGGTTTTGCCAACAGTTTTAGTTGTCAGATTTTTTATTTGAGATTGCTAATAATTAATGACAAAACTCCCTAAATTGTTACTGGGGTTAACCTTTTCTGTCTCACTTATACCGTTTTCTAGTTTGCTTATTACTAGTACTGATGTCAATAAGCAACCGGTTCCAACTGCCTTACAACGCACTGGCGCCTCTGCCATTCATGAAGGTAGTTTTCAAACTATTACTTTAGGTCAAAGTTTAATGGAGCAAATTGAACAGCTCCAACAATTTACGCCAGCGCAGCGCTTTACCCAATTTAAAAAGAAATTTCCCAACCAAAAATTACTCAGTCAAAGTGAGTTAAGTCCAGTTGATGTTTACAATTTTTTAAGTGGTTGACAAGATGCTTTGGTCTCTTTCTTGGATAGAGTAATTAAATTACAAGGCAAGGTTAAGGAAGCAAATGAAATTTTTAATCCAAATGTAGGTGATCAAATAGTTTTACCTAAAAAGGAAAACCCGAATGTTTTGGAAGTATTGGGCGAGTATAACGGTTTTGGTTTCTTCCCTACATTGGGTAAAAACGGATTAAACCTACCACAGCAAATCTTTGAAAACTTTACTGACTTTAAAGTAGAGAGTTACCAAATTAACGATTTCAAGGTAAGTCTAGTGGGCGAGCGTGACATTATTAAAAACGACAAAGTCCGTTTTTCTTACGCGGTGCAAATACCACTCAACTTAGAACTGCTTGTTAACAACCAAAAGGTTACTTTCAATATTACGGTTGATTTGAGAACCAATAACTTTTCTACCCAAGAGACTTTCAATGATTTATTCAACAATGGAACTGCACCAACCAACTGACAGTTTTTTAGTCGAATAAAGGTAAATAAGTTGCACTATGACCAAACTGATGCCACCCATTTAGCTAACACCCTACTACAAGACCAGTTTAATGCGTTAAACCTTGATTTACAAAAATCGATTTACGACCTTAACCTTGATGATTTAGAAGAAAGGTTTGAAGAGGAATACGCTAAGCCACTTCGTGAAAAAAGAACTCAGCAAAAGAAAGAATGGGAAGAGGAATAGCGAAGACGGGCAGAGGAAGATGCTAAACTGCAAAAAGAAATTGAAGAACAACAAAAGAAACAAAGAGAACAAGCAAAAAACGATGCTGAGCTCAAAAAAGCACAAGATGATCTCAAAGTTGCGCTTAATAGTCTTGACGGTTTTGTTAAATTCTTTACATCAAACGAAGGTCGCTTTAAATTAGGTTTTGAAAAGACAGATGACATTAAAACCCGTGCAGGGTTATACAAGGCACTGAAAGTTTCTTTTAGTAACTACCGTGCTTGAACCTTCTATCTAACTTTATTAGGTTGGAAGGAAACTACCGCAAAGATACTTACAAAAGCTAATTGAAAAGATATAAGAAGTGACGCTAATTTACGCAAGGCCTTCGGTTTAAAAGAAAGTGCCAACGAAAAAGAGGCTTCCAGGGTCATTACTCCGGGTTATGGTTACCAAGGTATTCACTTAAAACAATCGTTGCGTGATGCGATTGATAAATACGCTACTTACAGCGTTTCTCGACTAAATAACGTCACAACTTATTTACCTGGCAAAGTTGATGGAAGTGGAAAAGATCAAATTTGAATTTCGCCAAACAACTTTCAAGTGAATCGGGAATGGGGTAATGGTGCACATTTTAAAGATAAAGCATATCGCTTTAATTTTGATGTCAAAGTTGAATATGATGTAGAGGTAAAAGCTGCTTGATGAACTGCTCTTTTCCGAGGTAGCATCCCAGGGTATTGAAAAGGTAAATTTAAAGTTACTTACTCCTTTAATGGTGAAGTACCTTCTTGAAACTATGGTGACAAGCAAGTGCGTCCACCACAATACAGTTTTAAGGAACAAGAAAAACAACTTTTATTTGTACCACGTCATGTACAAAAAATTGAAGCTGAAGGTAAACACCTTGAAATAATTAATCCGTTCTTAAAAGATCAACATTTAGATTTTTTTGAACACTACCACCCAGATTTAACGCAGCCTTTAGACATGGTGTCATACCTAATGTATGCCATCGCTGACAAGGTGAAATAACAAAAACATTTTGTCTGACAGTTGTAGCGACTCTTATCCATTTCTCAATTAATTATTAGTAATGAAAAAACTCCACAAAATTTTATTGGGTTTATCTTTGCCCGCATCTTTGGCGCCCTTATCTGGGTTTATTAGTACTGATGCTGATAACTCACAGTTTTCACTAAAGAAGTCTAGCACTGAAATTAAAGGTACACAATCCATTCACCTGCTTAATTTAGGGGAAAGTTTAACTGAAGAAATTAAAGAGGCACAAAAGCGTACCCCAGAAACTAGTTTTGCCAGTTTCAAACAAAAGTTTCCCAATAAGGAAAGTTTTGTTAAAGGTTTTCAACCAATTGATGTCTACAACTTACTAAGTGGTTGAAAGGATGCCATTTCATCCTTTTTAGATAAGGTTGTTGAGCTACAGAAAAAGATCGAAGAAGCTAATAAAATTTTCAACACTAATATTGGCAACCAAATTGATTTACCCGAAGATGAAAATCCCAATGTGCTAAACGTTTTGGGCTCATATGGTGGTGAAGGGTTCTTCCCTACATTGGGTAAAAACGGGTTGAACCTACCACAACAAATCTTTGAAAACTTCACTGATTTCAAAGTTGTTAGTCATAAAATTCATGACTTCCAAGTAAGTTTGGTAGGCGAACGTGACATTATTAAAAACGACAAAGTCCGTTTTTCTTACGCGGTGCAAATACCACTCAACTTAGAGCTGCTTGTTAACAACCAAAAAGTTACTTTCAACATTACGGTTGATTTGAGAACCAATAATTTCTCTACCCAAGAAACTTTTAACGAGCTGTTCAATAAATGCATTGGCCCCGTTAACTGACAATTCTTTAGTAGGGTTAAAGTAGATAAATTGCACTACGATCAAACTGACGCTACTCATTTAGCTAACACGTTGTTACAAGACCAGTTCAATGCGTTAAATCTTGATTTAGAAAAGTCCATCTATGACTTGGAACTACCTAGATTAGAAGCAGAGTTCCAACAAAAATACGTTGATCCTTTAATTGAGAAAAAGCAAAGACAAAAAGCAGAGTGGGAAGAAGCGGAACGTATCCGTAAGGAAGAAGAGGAAAAACACCAAAAGGAGCTTGAAGAACAACAAAGAATTCAAGCAGAAAAAGCGAAAAATGACGAACAGTTACGTAAAGCGCAAACTGAATTGAAGAAGGCGCTTGGTGGCATCGACTCCTTTGTTGAATTCTTTACGAATAATGATTTACGTCTTAAACTAGGTTACACCAAAGAAGACAACGTTAGAACCCGCGCTGGACTTTTTAGAGCACTAGAAGTTTCCTTTGGTAATTACCGCGCTTGAACCTTCTACATTACATTATTAGGTTGAAAGGATACTACTGAAAAGATCTTTAAGAAAGCTAAATGGCAAGACATTAGGGATGATGAAAAATTCCGTAAGGCCTTTGGTTTAAGTCCAAAAGCCACCGAAAAAGATGTTGGTAAAGTAACCAATCCAGGTTATGGTTATCAAGGGATTTACATAAAGGATTCCCTGCGTGATGGAATTGCTAAGTACTCTGATTCGACGGTGTCTGAACCTAAAAACGTTAAGGTTTCTTTACCAGGTACTGTTGGTGATAATGAAGAGGGTAAGATCTGAATTGCTTCCCATAACTTTAGACAAAACCACGAATGGGGTGCTGGTGAAAAATTTAAATATTCAGCTTATCGTTTTAAGTTTGATGTAACAGTGGATTATGATGTAGAGGTAAGTGCTAAATGATGAACTTGAGCCTTTCGGGGTAGTATTCCAGGATATTGGAGGGGTAAATTCAAGGTTACCTATTCCTTTGATGGTGTCGTACCTTCTTGGAAGTATGGTCACATCCAAGTGCGTACGCCACAATACAGCTTTAATAAGCAGGAACAAAAGATTCTGTTTGTACCCCATGCTATCCAAAAGATTGCTGCTGAAGGTAGCAACTTAGATTTAATTAATCCATTCTTAAAGGATCAAAAATTAGATGAATTTGAGCACTACCACCCAGATTTAACTAAACCATTAGATTTAGTAGCTTATCTGTTATACGCAATAACCACGAGATCATAGTTGCCTAGTGGGATAATTAATAGAAATAAGAGCTTCTAGGCTTTTTTCATTTATTACAGCTTTTTCTTGGCTGATAAATTTACAAACAACTGCATTTGCCCATTTTACAAAAGTGCTAAATCAAGGGCTTTATTTGAAAATTGACCCGTTAAAAAGTTAATTAATGGGAGGATAAGCAAGCTTTAAAAAGCGCAAAATAGCGTTTAAAAAACACCTACTGTTTTCAGTTTTATTTTGAAAAATTCTAAATAAGAGATATAATTTTACTGATAGCTTTTCTTAAAAAATAACTTTTTGAGAGGGCGAAAATTCTTTTTACTAATATGTTTAATTTAAGTGATTTTAGTGAATTACCACGTTGGATTGGTGCCGAATTTTTAGGCACATTCTTTTTAATTCTTTTAGGTAACGGTGCTGGTTCGCAGCTAACACTCAATAAGATGTTTGCTAAAGAATCGAAAGCAAAGTTACTAACTGCTGCTTTTGCTTGAGGTATTGCTGTATTAGTTGGTGTTTTAATAGCTAACTCTTTGTTTGAGGGTGCGGGTAATATTAACCCGGCAGTGAGTTTGTTCTATGCTGTTTCGGGAACTATTCAAAAAGCTTTATATCCATTGCATGTAAACTTCAGCATTCCATTACTTTGAGTAGCACTCCTACTTGCTTGAGTAGCACAGTTTGCTGGGGCAATGTTGGCACAAGCATTGTTAAACTTCTTGTTCTGAAAGCATATTGAACAAACTGATCCACAATCGGTTTTGGTTACCCATTGTACTAACCCAGCAATCTTTAACATTCCTCGCAACTTTGCAACTGAGTTTGTAGCAACTAGCGTTTTAATTGCTAGTTTACTGGTGGCTGGTAGTTTTGGTGCCAACCGATTTGACCAGTCACCACGAGGGGTTGTACCAATGTTAGTGGTTACTGGCTTAATTATGTCTTTTGGTGCCGCTACTGGTACTGCAATTAACCCAGCCCGTGATTTAGGTCCGAGAATTGTTTACTGATTGAGTCCAATTAAAAATAAGGATCCTAACTTAAAGTACAGTTGGATTCCGGTAGCTGCACCTCTAAGTGCTAGTGTTATCTTAGGTGTGCTTGTGGCTGTTATCGTTTAGTTCCTTAAAGCGTTCTTTAAACTGCTTGGTCTTAACTGTAACTGGCTTACCCTGCACGTGGTGGTGCAACTTACAACGTGGTTCATAAGCTTCAATATCACCAATTAAAACGGTTTCGTTGCTCTTGGACACTATGCGCTGGGTGCGTTGGGCTAACTGACCACAGACATTACAAACGGCATCTAACTTACAAATCTTGTCGGCAATCGCTAATAGTTGGGGAATGCTACCAAAGGGTTCAGCGCGGAAGTCCGTGTCTAAACCAGAAACAATGACGTTAATGCCGAGATCATTGAGTGATTTAACCACTTCGACAATTTCACTCGAAAAGAATTGGGCTTCATCAATGGCAACCACATCAAAACGATCTTTAGTGAGGTAGTCATAGATTTCTAAAGGTGAATTAATTTCAATGGCTTCATCACTGTGACCGTTACGGGATTTCACGAGGTGTTGTTGGCGCGTATCAATCTTTGGTTTAAAGATAATAACGGGAATCTTGGCGAGCTTTCAGCGCTTAATTTTGCGCAATAATTCTTCGGTTTTACCGGAAAACATCGGCCCACAAATCACTTCGATTCAACCACGTGGGGATTGGTGGAAAACTTGGGAAAAGCTCATAGTCGCCCAATTATAATTAAACAAAGATGAGTGTGTTGCAAAAACCGCGCGGGGTTAAAGACTGGTATGGTGAGGAGTTAATCTACTTCAATTGAACGGTGCACCAGATCACTAATTTGGCCTGAAAATGAGGCTTTAGTGAGGTCAAAACACCCCTGTTGGAATACGCTGAAGCCTTTAAGCGCACCAATGCCAACGCCGATATTGTCAAGAAGGAGTTGTATGAATTCCATGACAAATCGAACCGGCTGTTAGCCCTGCGTCCCGAGGCTACTGCTGGGATAGTGCGCTTGGTCTGTGAGAACAAACTACTCCAACCACAAAACTACCCGTTAAGGTTGTTTACCATTGGCACCATGTACCGGTATGAACGCCCCCAAAGTAACCGTTACCGCGAGCACTACCAGTTTAGTTGTGAAGTGATAGGTGACACTAATCCAACCGTGTTACTCGACACTTTACTGTTGGGTCATGCCATTATCCAGCAATTGGGAATTGAAGGAGTGATCCTCAAACTTAACAACTTAGGTAACAGTGCTACCATTCAACAATGAAACCAAGCGCTGCAAGCTTACTTAACCCAGTTTAAGGCGCAGTTAACCGAGTTATCACAAAGCCGTTTATCGACCAATCCTCTAAGGATCTTGGACGACAAGGTTGATGGTCAACTACCCTTTATCAGTGATGCCCCCCAAATTGAGCAGTTCTTGGATGCTGAGCAGCAGGCACTGAATACTTGACTGCAGCAACAGTTAACCCAACAACAGGTGCCGTTTGAGTGGAATCCAACCCTAGTCCGGGGCTTGGACTACTATACTGGGGTAGTGTTTGAATTTGTTAAGGATGACACCACGGTACTAGCTGGTGGGGTGTATGATAACCTAGTGGAAGAGTTAGGTGGTACACCTACTAAAGCGCTGGGTTTTGCTTGTGGCATTGAACGCTCAATTAATTGTTTAAGTGCGGTTAAAAAGCAAGCAATTTTGGCCAATCAACCCCCACGATTACTGGTTATAGGTCTCACCGAAGCAGCGTTGGAAAAGCTGTTACAACTTTCATTGGGTTGAAGGGCGTATCATCCTGTGACTATTTATCCTAAAGTTATTAGAATTATCAATGGCATCAGAGCCGCGCAACGGTTAGGTTACCGCTTTTTAGGGGTCATTGGTGGTAATAATTTAGAACAGCAAACCATTACCGTTAAAGACCTGGCTACTGAACAGCAAACAACATACACTTGAGATGAATTTAGACAACGCCAAGTGCTTTAAGCAACGGGTTTTTATTGGCAATTTAACTTCCGAGCACCTCAATAAAACCGTTACTATTGCCGGATGGGTCAAACGGATTAAGAAGTTAGGTGAACTTAACTTTGTTATTGTCGGGGACAAGACTAATACTATTCAGGTTACCTGTAAAAATAAGGAACAGGTCAAGTACTTAACCAGGGAAGACTTGGTGATTGTCAAGGGCAAACTGAAACGCTTAGACAGTGTTCGGTTTGAAATTACCAATCCTACTATTACCTTATTTGCCAAGTCCAAAACACCCCCTCTTATCATTGAGGACAAAACTGATGCTTTAGAAGAAGTGCGTTTGCGTTACCGCTACTTAGACTTACGCCGTCCGGTGATGCAAAAGCGCTTGGCGTTACGCCATAAGGTAACTTTAGCCGTCCGGAACTGACTAGATCAAATGGGGTTTATTGAAGTAGAAACACCTACGCTCACGAAGTCCACACCGGAGGGTGCTAGGGACTTTTTGGTACCGGCCAGAATTAGGGAACATAGCTTTTACGCTTTGCCCCAAAGTCCACAAATTTATAAACAGTTATTAATGGTTGGTGGTACGGAAAAGTACTTCCAAATTGCCCATGTTTACCGCGATGAAGACAGCCGCAAAGACCGTCAACCTGAACACACCCAAATTGACTTAGAGGTGGCCTTTTACACCAAGGAAATGGTGATGGATTTAATCCAAAGGTTATTTGTCGATGTCTTTCGCCAAGTGTTAAACATTAAGCTCAAAAAACCGTTTCCAGTGTTAAAGTTTGCTGAAGCCTTTAACCGTTTTGGCAGTGACAAACCGGATTTACGTTATGGTTTCGAACTGGAAGACTGCACTGATTTATTCCAAGACAGTCCGAACCAATTCACTAACTTAATTAATGCTGGCGGCATAGTCGGTGGGATCCAATTACCGAATCTTTATTTAGACGAAGTTTCTTTTAAAGCATTGAGGAAACTAGCTAAGGACAACGGGGTTTCCTTAGAGTTTTACTCTGATAAAGCTAGTTCCCTAAAACAACCCCTGGATTTACCGTTAGCAGGCACCATCTTACTGGTAGCACACAAAAGTAAAACGCAAGCTTGAACGGCATTAGGCGCCATTCGCAATGAGTTGAAGTACCACCTCAACTTAGTGAAACCCAACCAGTACAGCTTTTGTTGAATTGTAGACTTTCCTTTATACGAGTTTGACGAAAAGGAACAGAAGTGGGTTTCGGCGCACAACATGTTCTCCAATCCCCAACCGCAGTGGTTGGTTAACTTTGAAAACCACAAGGCTGAGGCGTTGAGTGAACAGTACGACCTAGTTTTAAATGGGTTTGAACTGGGTAGTGGTTCAATTCGAATCCATGACCCAGAAGTACAAACCCGTTTAATGCAATCTTTAGGGGTGGATCCCCAACAGTTTGGCTTTGTGATGGAGGCTTACCAGTACGGTGCTCCTGTACACGCTGGCATGGGTTTGGGATTAGACCGCTTAATGATGATCATTAACAATGTGGACAACATTAGGGAAGTGATGGCCTTTCCGAAAAATGCCCAAGGGATAGAGATGCACACTAATGCCCCTGATCAGGTTGACATTAAAGACATAACAACAATATGGTCCAAACACCCAGTGAAATAAATACCCACCTTAAACACCTCTTGGCCTGTGATGCTTATAAGCTATCACACCGCTTAATGTATCCTAATGACACGACCAACTTATACAGTTGTTTAACAGCCCGGGGTGGTCGTGGTGGTTTTCCAAATTTTGTGTGAAACCACGAGTTTGCCAAAAAGATTATTCTTGAAGTGTTTGGTAACTTTTGTGATAGTGTGTTAGCAGTCCAAAACGATCCTGGGTTGGCCCAAGCGCTCACTGACAAGGTGACCACTGTTTTTGGTGATCCCCAGTTTGGGTTGGAGTTTACCCAGCACATCTGTTATTTAGCTAACTTTTTAAAACAACACCACCAGTTACCCTTAACGGTAAAGATTCACCAGAGTTCTGAGGGTTTAGCCTTCCGTACACCCTTAGTAACTATTACAGGTTCGGACCAAATGGTTCCCGAGTTAGTTTGGTTAGTCAACTATTTTGAAACCGTATTATTGGAAAACATCTGATTGTACCAAACTACTTTAACTGTCGCCCAAAGCTTAAAGCTGCTGTTGGAACGCTATGCTAACGAAACCGCGGATAATACTGAATTTACCCACTTTCAGTGTCATGATTTTAGTATGCGTGGTATGAGTAGTCTTCAAAGTGCTTTATATGTAGCTAATGCTCACCTGCAGTACTTTAGTGGCAGTGACACCATTTTAGGCGGTGTAGCTGCTAAATCGATCTTAGCTAGTGAACACTCGGTGATGTGTGCTGATGGTCAAGAGGGTGAATTGAATACCTTTAAACGTTTATTGGAGCAGTTTCCTAACAAAAATCTGTCCTTAGTGATTGATTCTTATGACATGTGGCATGTTCTAGATAACATCTTACCCCAACTCAAGGACTTAGTTTTACAACGACAGGAGAAGCTGTACCTCCGACCTGACTCGGGCAACTTTGAAACCCTTATTTGTCAGGGCAAACGGTTTAACCCCGAGGACAAAACTACCTGGGGAGTAATTGATTACTTAGATTACCACTTTGGTTCCACTGTTAACCAAAAAGGTTATAAGGTTTTGAACCAAAAGTTGGGTATTGTTTATGGCGATGGCATTACCTATGAGCGGATTGAGTACATTTTGGAACAGCTCAAACAACGTGGGTTTTGTTCTTCCAACATTGTCTTTGGGGTTGGTAGTACAACTTACCAAAACCTTAACCGTGATACGTTGGGATTTGTGTATAAGTTGACAGCCATCAAAAAGGGTAATACCTGACATGATGTTACCAAGAGTCCCATAACCGATCCCACCAAGCAGTCCATAGGGGGACGATTTGATAACCCCAACCTAATTCAGGTTTACGGTTAATGCACCTCAAAAAACCGTTAGTGGTTACTAAGGATTGCTTTTATTGACTTAGTTTCGGATCGCTAAAATGATTGTTAGCACCAATCTTTTTAAAATTTTCCAAACATTAAATTTTCTTTGTTGGTTGTAGTTGTTAGGTTGTTTAGTCAAAGATCCACCTTTTCGTAATAGTTAATTAAGTAATTTAGTTAGTTTCTTTTTATCCAATTTTAAACAGACTAAAAAATCCGCTTTCGGTATAAAGCGTTTCTACTAACTTTATTTGCGTCAACCACAACGCTTACTGGATTCATAATACCTACTTTGAGCCAACACGGTTCGAGTACGGATCCGAGTTTTGTGGTATTGCGCAACAACAGTAGTTTAAGCCGTGATGGTAAAAGTCGGCTTTTAGAATTTGGTAATGACCTTGGTTTTAGCAAGGAAGAAACTGATGCCTTTGCTAAACAAAAGGATTGAAACCAGAACTTTGTTAAATTTCAAAAGCAGTTTGAAAACAAGCTTTTAGACCCAAAGAACTTTAGCTTAACCGATGTTTATAACCTCTTTAGTGGTTTTCAACAAAGTGTTACGGCTACTGTTCAGCTAATGAACGAGTTGCAAACCAAAGTCAATGAGGCTAATAATATTTTCCCAGTTGAAGCCTTCAAGGTACCTAAGGTTCCAGAAAAACTGTTTGGTTTTGTCAACCAAGGCTTCTTTCCTAAACTAAACCCTAAGGGCTTAAACATTGCTGATAACGTGGCTTCCTTATTTGAACAATACAGCTTAAAGCAAGCAAGTCTTAAGGACTTTGACATATTATTGGAAAAGAAAAACGACATTGTGTTGGAACACAAGGTGCGTTATAACTTTGCTTTGCAATTTAACTTTGAAACCACTTATGTTGGTACTGGTGGGGAAATTAACCTACAGTTTGCACTGCAAGCTTCCACTACTAACTTTAGTAGCTTAGAAGAACTCCAAGCTAGCTTTAGTAAAACAGGCGATAACCTTACTGCCCAATTGTTCTGAAAACCAACTGTAACTAAGTTAGTTTCTGGGGAAAATGATCTAACCCATATTGCCCAAACAGCAATCGGTGAAAGTCTGTTTGACAGCCGGGTTGACTTAAGTGCTTCTATTATTAATAGTGAAGCAACCCTTAAAACAGCCGAAGCAACATTTACAACCCAAGTTTTAAATCCGTTTAAAGCGGAACGGGAAAAGGCTTTGGCCATTAAGAAAGCTGAAGAAGAGAAAATTAAAAAAGAACTTGAAGAGCAAAAGAAGCGTCAAGAAGAATTAGCAAAACAACAACGAGACAAAGAGGCATTACAAAAGTCACTTTGAAACTTTCAAGAATTTATTAGCTACTGAAACGGTCAAGGAAAAGACGTTAAGCAAAAAGAACAATTTATTCAAGCATTAGAAGCGGCGTTTTCTACCAATTGGAACGAGGTTTTCAATTTGTTAATTGCCGGTTTTAGAAGTGCAATTCAGACTTATTACAAAGATGGCAAAGCAGATCAAAGCCAAAACGCTAAAATTGCTTTTGGCGAAAAGGGTATTCAATTCCCTAAATCTGGCCCAGGACTTGACGGTATTTTCATGTCAGATTTCCTTCGTGGTAACCTTACTGGCAACGCTCACTTTGATTTAAAACTTAAAAAAGTTGAAGTTAAAAACACACAAGGAAAAGACGCACAAGGCAATGATAAAAAAGCCTCCATTAATTGACAAGCTAAGCAAAATAACTTCCCCTTCAGACAAGTAAACCCATGGGATTTTTCCTTTGAAGTAGAACTTAAGTATGAAGGTAGCTATGGCCTATATCCAGGTGCTAGGTTCCTTAACTTATTTGGTTCCTTAGGTATTCCTAACGATTGAAAAGGCGAAATGAGCGTTAAGTTTGTGCTCGATGGTAAGACACCTCAATGGATTGCTGATAAACCAGATTACCCAGGTTCCTTGTTTAAATTTGAAAAGAACCAGTTAAAGTTCACTCCCCACGTTAAGGAGCATGTACATGTTGAAAACAAACAATTTATGGAAAAGTTAAAGAGTCAAAACTTGCATAACTTGGAGTTAGCAACTGGTGCTACAAAACCACCTGTTGTGGACTTAGCTAGTTATCTACACTACCTCATTTTGAACCACAAGTAGATTTAACGTATTGCTAAATTGGTTGTATAAGCCAAACTTTATACAATCTTTCAAACATTAAATTTTCTTTGTTGGTTGTAGTTGTTAGGTTGTTTAGTCGAAGATCCACCTTTTCGTAAAAGTTAATTAAGTCATTTAGTTAGTTTCTTTTTATCCAACTTGAAACAGACTAAAAAATGCGCTTTCGGTATAAGGCGTTTCTACTAACTTTATTTGCGTCAACCACAACGCTTACTGGATTCATAATACCTACTTTGAGCCAACACGGTTCGAGTACGGATCCGAGTTTTGTGGCATTGCGCAACAACAGTAGTTTAAGCTGTGATGGTAAAAGTCGGCTTTTAGAATTTGGTAATGACCTTGGTTTTAGCAAGGAAGAAACTGATGTGTTAGCGAAACAGAAAAACTGGGAGCAGAATTACACTAACTTTAAAAAGCAGTTTGAACACAAGCTTTTAGATCCGAAGAGCTTTAGCTTAACTGATGTGTATAACCTCTTTAGTGGTTTTCAACAAAGTGTAGCTGACACGGTTAAGCTAATGAATGAATTGCAAACTCAGGTAAATAAAGCTAACGACATTTACCCAGTGGAAAGCTTTCAAGTGCCTAAGGTACCACAAAAGCTATTTGGATTTGTCGACCAGGGCTTCTTTCCGAAGTTAAACCCGAAGGGCTTGAACATTGCTGATAACGTGGCTTCCTTGTTTGAGAAGTACAGCCTAAAACAAGCAACCCTTAAAGACTTTGACATCGTTTTGGAAAAGAAGAACGACATTGTGTTGGAACACAAGGTGCGTTATAACTTTGCTTTGCAATTTCACTTTGAAACTACATACATAGGTAGCGGTGGCGAAATTAACCTACAGTTTGCACTGCAAGCTTCCACCACTAACTTTAGTAGTTTAGAGGAATTGCAAGCTAGCTTTAGTAAGGTAGGTAATAATTTAACTGCTCAATTGTTCTGAAAACCAGTTGTAAACAAGCTCACTTCTGGAGAAAATGACTTAACTCACATTGCTCAAACCGCCGTTGGGGAAAGTTTATTTGACAGCAGGGTTGACTTAACATCCTCTATTATTAACAACGAAGCAGCCATTAAAACTACTCAGCAACAATTTGAAACAGAGGTTTTAGCACTTTTTAAAGCAGAACGTGAAAAAGCTTTAGCGGAATATAAAGCAGAGCAAGAACGAATTGCTAAAGAACTTGAAGAACAAAGAAAAGAATTAGAACGTCTCAAAAAAGAACAACAAAATAAACAAGAGTTAGTAGAGTCCTTATACAATGTTGCTAACTTTGTTAGTTATTGAGAAAAGCGTGGCAAGGACGTTACTGACAAAAAACAATTAATTCAAGCACTTAAATCAGCTTTTGCTACCAACTGAAACGAAGTGTTTCAATTATTAACTGCTGGAATGCGTGAAGGTATTAAGGAGTATTACAAACATAATAAACCTGACCAAAGCGCTAATGCTAAAAAAGCCTTTGGCCAAAATGGTCTTGCATTTCCGCGAACTGGCTTTGATGGTATCTACATGTCTGACTGGCTTCGTGGTGAATTGAGGAATAAGGGTAACATCAATCTCCATCTCAAACAAAACGAAACCACCGTTAAAAAGATTAGGGATGACATTAGTATCGAATGAAATGAAAGCAAGGGCGGTATTGAGTTTCACCAAACTTACCCTTACTGATTTGAATTTGAAGTTAACTTTAAGTACATTGGTGGTTACTCCTTAAACTGATGGGATGCCATTTGAGCTAAAGTAGCTGGGATCCCAGGTTCTTGAAAGGGTGAAATGAACTTAAAGCTAGTTATTGATGGTGAAATTCATAAGTGAATGGTAACCAAGCCAGACTATCCTCGAACCTTCTTTCAATTTGATGACCAGTTTGATAAGCTTTGATTCACCTTGCACGTTAGTCAAGAAATTAGTGTAAGGGATGAATCCTTCATGAATTTGCTCAAGAAACAAGGATTAGATAAATTAGATCTTCGAACAGGATCAACCAAACCACCAGTAGTCGATCTAGCTAGCTACCTCCACTACTTGATTTTGGCAGACAAGAGTTAATAACTAAATTAACTATCAAAAAGTTTAAGGGCATCTACCTAAATAGGTGTCCTTTTACTTTACCTTTCAAACTAAACAAAAAATACTCTGTTGGAGTAGATTTTTTCCTCTCTAACCCTTTTAAGTATTGGTTGTAAGTGGTAATAAACGCTTCGCGATGAAAATCAAAAGTTCATTAGTTTAGGTTAATAAATTTAAGTAAAAATTAAATATGCTTAATTGATTTAAAAAAATTTTTTTCTCTGATTGAGGTGGAGCAGAAGATAAGCAGAATAAAAATAAGAAAAATGAACAAGCTGTTTTTGTAGATAAAGTAATGAGTAGAACAGATTTAACTGATGAAGAAAAAACAAATTCTAGTTGATTACTATCAGATGTTAGATGACTATTTGGACGATGATTGAGACTAACTAAAACCACCCCGTGGGGTAGTTTTTAATTTTAAGAACTAACCTTGTCTTAGTCTAAGCTAGCTCATTTTAGTGTTCTTTTAACAGCTTCGTTTCAACGCTTTGATTTCTTTTCCCGCACAGTTTGGTTCATTTGACTTTTGAACTGCTTAGCGATTCCAGTTAGTTTTTCTAGTTGTTTAGTGTCCTTTCAATAATTAACTGCTAAACCAGCTAGGAAGGCCGCACCCATGGCAGTGGTTTCCACATTCTTAGGAATAATCACCTCTAAGTTAGCAATATCAGCTTGAAATTGCATTAAATAGTTGGAATTGCAAGCACCGCCATCAGCCTTAATTTTTTTAATAGAACTGTTTAAATCACTAGCCATTGCCTTAATCAGGTCATTAGCTTGAAAGGCAATTGCTTCCAAGGTAGCTTTTACTAAATGTTCCCTTTTGGTATTAGCTTCTATCCCAAAGATGGCGCCTCTTGCTGAAGCATCCCAGTAAGGTGCGCCTAAACCAGTGAAGGCTGGGACAAACACCACTTCCTGTTCTTCCTTTTGTGCTAGCTTAGCATAAAAGTCACTTTCAGCTGCCGAGTACATTACCTTAAGACTATCCCTTAATCACTTTAAGGCTGCGCCAGCCACAAAAACACTACCTTCTAAGGCATACACCGGTTTTTGGTTTTCTAACTGCCAAGCTACAGTTGTTAATAAGTTATGTTGGGAGTATTTCAGTTCGTTACCGATGTTCATCAACATAAAACAACCTGTACCATAGGTGTTTTTAACCATCGCTGGTTCCACGCAGAGCTGACCAAACAAGGCTGCTTGTTGGTCACCGGCAACCCCACGAATAGGTACCATACCAGTAGCACTAGTAGATCAATGACTCGGTACTATGTCACCAAAGTGGGCATTGGATGGCATCACTTTGGGTAGGATTGATAAAGGTACTTTAAAGATGTCGCCTAACTCTTGTGATCATGTCATGGTGGTAATGTCAAACAAGAGCGTACGCGATGCATTACTTACATCAGTTACATGCATCTTACCACCCGTTAGTTTTCAAATTAACCAGGTATCAATTGTACCGGCTAACAACTTGTCTTCCTTAAGCATCTTTTGCGCTAAAGGAACATTTTCCAAGATTCAAGCAATCTTAGTGGCACTAAAATAAGGGTTAATTGGTAAACCAGTGTGTTTCTTGACAAACTCCTTTATCTTGGTGTCTTGGTTTAACTTATCACACAAACTAGCAGTGCGTTGATCTTGCCATACTATTGCATTGTAAACGGGCAAACCATTTTCTTTGTTTCATAACACAATGGTTTCCCGTTGGTTGGTAATCCCTACAGCAGCAATGTTGTTTGATTTAATTTGTGCCTTATTTTTAGCACTTTGCATCGTAGCTAATTGGACTGATCAAATTTCTAAGGGATCTTGTTCGACCCAACCACTCTTAGGAAAGAACTGGTTAAATTCGTTTTGGGCAATCGCTACTTGGTTTAAATCCTTGTCAAACACAATCGTGCGGCAAGAACTGGTTCCTTCATCTAGAGCAAGAATGTATTGTTGTTTTAGATCCATGGCAGATTGTACTTTCCGTTTCAGTTCGGCTTTTTAGTTAATTTCCAATTTAAAAGTCTGACAGCTTCCCGGGCAATGGCTGGTGCTGCAGTTAGCCCTGGGGATTTCATACCCCCTAAAATGACAAAGTCAGGGTCATTGTGAGCTACTCTAATAATAAAGTCATTAGTGGCTTTGTCAATTGGACGCGAACCAGCAAAGCTTATTAAGGCATTATTAATGTTTAGACTTGGCACCATATGTTTGCCAATCTTAGTCAACATACATGGAGCATCTTTGGTAATGTAACGAGTAGCTTCCTTGTCTACCCCATCTAAAGCGGTGGGTCCGACTAAGAAGTTGCCATCTAACATGGGTGACACAATTACCCCTTTACCATGGATTGTTGGTACCATGAAAACCATTGAGTTTAAGTGGAGTTCGCCCTGATTAGTTACCACCACATACTGACCTCGACGGGTTGTTTGTTCAAAATCATCAGCTTTGGCTAAATGAGCTAAATAATCAGCATAATGACCTGCAGCATCAATAATCTTCTTAACTTTAAAACTAGGAGTAGTTTCGTTGTTAGTTCAAACTAAATAAGTCCCATCAACTTGCTTACTAATATTAGTAACCTCAGTATTAGTGCAAATTTGTACTTTGTTTTGCTGTGCTGCTAAAGCTAAACATTTGCTCGCTAACACTGGATCAATTGCTCAGCTACCTTCGATTTTTAATCCAGCCACAATTTCCGGATTAACGTAAGGCTCTAACTTTAAGGTTTCTTCTTTAGAGAGCATTTGAATATCTTCCAGATTAATTTGATTGGCAATGCCTCTTTGCTTTAGTACTTCTAATTGTTCGCGCTCCATTTCATTGAAAGCCACAATTAAAGTACGGATCTTTTGCCTTGGAAAACCTAAGCGTTTAAAGTAGGTATTTAATCAGAGCTTTTTACCCAAAATGTTGTATTTAGCAGTTAACTTGTGTGGATTGGGATCAATGCCAGTGTGGATAACACCACTGTTGGCATGGCTAGTCTCTTGTGCCAAATAATGGTGCTTTTCTACCAGTGTCACTTTGAGCTTGTACTGGCTCAATTCATAAGCAGTGGCACAGCCTATTACACCACCGCCAACTATTAAAACATCTCTTGTTTCCATAAAGGCTTAACTAATTAATAGTTACAGAAATGCCTTGACACGCCGATGCTTTTATCGTTTAAAGTTTTGCAGAGCTGAAAAAATGGTTTTTTAAAAAACATTTAACTCTTATTTAAATACTTTGAAAATCAACGATCTAATAAGTTACTATTACGCATATTTTTTAGAAAGGGTGTAATTTTATGTTTGGAAAAGGGTTGGTCAAAAAAAGCCTTTTATTTTTTAGTGGCGTGAGTACTATGGCCGTCTTTTTAGTGTCTTGTGGTGCTACTAGAATTTGGGAATCTTCGATTCAACTGTTAGTTTCCAATGATGAAGCTACATTAGCTGACAAATCCTTTTCTGAGATGTCCTATGAAGGGATTAGAAGGTACTTTAGATCTCAAAAACATATTGAATTACCATCACCAAATAGTTCCCTGTTACAAGACGGCAACGGCCTTTGAAAACGTCCCGGACGTACTTTAAGTGATCGGATTGCTACCTTTAAAAACATTAAGAACGATGGTAGTGATGTGATTGTAGCTACTGGATTTAACCAACAAGAAGCACTACAAGCAATTAGCTCTGATGACCGTCGTTATTTAGCTGATAAAAACGACCTTGCTAAAGTTGGTTTCATTTTTGTCGATGGCCAAATTGAAAAGGAATATAACGTGATTAATAAAACGCCACAATTTAGATCAACCCCACTAAACATTTCATCCGTTGCCTTCCGCAGTGATGATGGTTCCTTCTTAACTGGAGTAGCAACTGCAGTTTATTTGAATTTAAATCAAGATTACTTTTTAAAGAAGAATGGAGCAACCAATAATAGTAGCCAAGATTTAACCGTTTCTGGTTTTGTCGGTGTTCCTATTCCTTCAACCCTTTCTTTTTTAAACGGTTTCCGTTTGGGCATTGCTTATTTTAATGAGGTGATTTACACCCATTTAAGTGATGCTGAAACAACATCTGATAACAAGAGTAATTCTTCAAGTGCTAGCAACTCTGTTTTAGTGCAACTTAAACAGATGCAAGGAAACGACAAAAAGATTAAAAAGATTAAATGGATATCTCCTAAACAAGGAAGCGATAATAATTCGAACCTCAGTATCGATGATCACAAGTCTGGTTCTTTCTCCAGCACTGAACCACGAGCAACAACTATCATTAATAATCTTTTAGACAAGGGTGTGAGTGCCATTATTCCTGTTGCCGGACCGCAAGTAAACTTAGCAGTTAACGAGGTGGCACGTCGCAAAGCGCACACTGCTATTATTGGTGTTGATAGTGCGCAAGAATTATTAGATATTAACCAAGATGCACCTGATAAAGATCAGTTAATCAAAGGTAATAAAAAAATTATTCCTTTCTCTTCTATTAAAGCCTTGGATGTTGCCATTGAAAACATGTTGATTGCCATTCAAAAAGGCAGTGATAATAATGGATATAAAGGATTTGGTTATAACAACATTGGTACGGTTGGAACTAGTTCAGTAGGTATTAGTGAGGCTGGTTATGAATTCTTAATTGATCCAGTTTTTTGAAAGACTACTCAGTCACAAGGGAAATCTATGGCCACAAATATGACTAATTTAAAACGTTTATCATCTGATGATACAAACACTAAGAAAGCATTAAAAGAGGTAAGCACCCATAAAAATGGTAGTGATAAAGATGGGATTATTGGTAAATATTCCAATTTATTAACTAAAAAAAGCACAACAGTAACTGCTGTTGCTCAAAAATCTATGACAGACAATAATAGTGGAACTGAGCAAAAAAAGAATCTTTCTGAAGTAGATACTAAGAAGAAAGAGAAAGAAAGTAAAGGTAAAACTCAAAGTAATGGTCAGGACAGTGGCCAACAAAACGGAAAAGAAACAAACGATATTATTTCTAAGTATTCTAAGCTGTTAACTATGACAACTATGAACAATAAAGTAATGTCATCTAAAAAACAAAGTAGCGATGATAACAGTTTTAAAAAGACTTCAGAAAACGGAGACTGAGTAATTAAAGGAGACGAGTTAACTAAGAAAAAATCAACTGAGCTTCCTGCTTTTGCTAAAGGTGCAGATTACCCTACTTTCCCTACAGAAGCGGTAAGCGTTATTAACGGAAGTACCGCTTTAGACGGCAAAGGTTTTAAGTGGAGCTTTAAACAAATTTAAAATAATACTCGGCTGATATGAAGAAGATTCAAGTAGTCGTTAAAGACCCTGTGGGCATTCACGCCCGTCCTGCTAGTATTATTGCTGGTGAGGCTAATAAATTTAAATCGGAATTAAAGCTAGTATCACCAAGCGGCGTTGAAGGTAACATCAAGTCGATCATCAACTTAATGTCTTTAGGCATTAAGCAAAACGACCACATTACCATCAAGGCAGAAGGAACTGATGAAGAAGAGGCACTAAATGCTATTAAAGCAGTTTTAGAAAAACACCAAGTTATTTAAGTAATTTAATTTAACTTAGAATAAAAGCAACAAGCCAATCCCCAAGGAAAAGCTTGTTGCTTTTTTATTAAGAAATATGCTTTACTACGACGACGAGGATGAATATGACACTTCTAATTCTGGTGGTGTCGTAGATTTAGCATTTTTAGCACTAATAGTAGTTTTTCTATTTACCGCTTTTATTTCTTGGTTTGGGGCATGAACTTGCTACAAAACAAGGCGTTTTTGAAAGGTAATTAATAAACCCATTAATGTAATAGCAACAGCTTTCTTTAATTTGGGGAACCTTACTTTTTCTTTGTAGCATTAACTTTTCTACTAGGAAATATACAAGCATTTACTCATGCTAATTACAAACATCCTAATAGTAGTAGCACAGAACTTCAGGGAATAGCAATTAATATTGCCTTAATTGTTGGCATCATTAATTTACCTTTATTTATATTCTTCCGAATAATGCTGAAAAGTACTCATAATTTCTTAGACAAAAACATTCCACTACGAAAGAAATGAATTGAAGAAAAGATTGCTAGTGATGAATTGGAAGGATGAGAATACACCCAAATTTATCCTTTCCTAAGAAAGCTTTCTAGAAGAAAGCGTTACCATTTTCTAAGAGCCTTAGAAAATGGTGATAAACCAACTTCTGTTTTAACAGATAGAGAAAATCAATTGTCTGAAAATCAAATAAGTCAAGCACATGCTTAGTAAATAGACACTTAGCAAATTTATGTTTGCAAGTCTTTTTAATTTTTTAGCATCAAATTCGTCAATAAACTCTCTAATTTTTACCTAAAAATTCCTTATTTATTTCCTTATATTTTTATTTAAGAATAATCTTTTTTATTTCTCAAAATTTCCTTGATTTTATCCAAAATTTCCAATTTTATTTCTGGGAATTTTTACTTGTTTTATCTAAAAAATTACACCTTTCTTTTTTAGTGTTTCTCAAATATTTCTCTAAGAATGAGATTTTTATTCTTTGGTATTTCCACGTTATTTATCGAAATAATCTCTTTTTATTTCTAGAAAATTACTTTGATTTTTGTCTAAGGATTCTCAACTTAGTCCCTAAATTTTCTCCGCTTTTTAGCGAAGAATATTTCAATTTATTCTTTGATTTTTATCTAAAAACTATCCCCTTAGTTACTTTAAATTTCTGTTAAATTTGTTTAGGGACTCTCATTTGTTTCTAAAAAACTTTTCCTTTTTCTTTCCAAAAGATTACAGTTTTTATTCTTAGAAACTTATCCGCCGTTTATCACAATATCTTTTTATCAAAACTTCTCCTTTTTATTCTTTAGGTTCCTCCTTTAATTTATCGAAATTTTCCATTTTTTCTTTTCTGGAATGTTTTTCTTTTTATAGCTAACTTAGTCTTTGAAATTTCTCTTTTTTATGTTCGAAGATTACGAATTGATAGACAAGAAATTGAAGATTTTTCAATTTTTCAAGCCTCCTTTTTTCAAAAAAACAGAATTGGTGAAGTTACTTTTTTAATTTTGTTCTAAAAGTAGCTTCGTTACCTTTCTAATTTAATGAAGATTAAATATTTCTTTATTCCCTTGTTTTCTTCAGCTATATTATTTAGTGCCTGTTCTTCTATTCAGTCTGATCTTAGGAATCTTATTAAAGAAACAACTGGCAAAGATTTTGACGTATCTAAATTAATTAAAACCTCTGAAGGAAGAAAAAACTTAATAAATTCTCTCAAAAAGTCTTACGAATCTAAGCCCGATGAAACAGCTAGCTTATTACTAATTGCTTGAAAGCAAAGTGCAGAAATGGGCGAAATAGGTTTTGATGATATTAAAGAAGGAGGAATTTATACCAGAGACAATGATCCTTTCAAATTGGAACAAAAGGTTGAGTACTTCAATATGGAATACAAAATTTTAGTGATGTTTTAATTAAAGCTAAATTGATCTATATCTTTAAATGGCATGGCGATCATTCTTCTTCAGAAGGCTTAACTGCTAAAAATGGCGGCAAGCATCATTTCGATCTTTTTCTTAATCTTAAATCCTCTTCAAAAAAACCATTTAATTCAAAAAGTTTTGATACAAAATCGGGAGAAGTTTTTCAAACGGAATGAATTAGTTTCCATACTTTAGTTAGTTGATCATTAAAAGGTAAAGATGAAAAAAGTCAAAAAAATCTTGACAAAATTATCATTAGCGAGTTTGTTACTAAAAAAGACCATGAACATGATTTTAATCTCTTTATATATTTAAACTATTCAATTAAGTAAGCTCTCTAGATTATTTAGTTTTGATTTGCTAAAAATTCAAGTACTTTACTTTCAGTTCAGTCTTTAAAGTTAGCTATTTTTTAGATCAAAGATTAAATTTATTGGATTTCTATCAAAAAATTCCTCTTTTTACTCTTTGAATTTTCTCTGTTTTTTATCCAAGAATTACACCCTGATTCCATTAAAAGTTTTTGATTTTTACCAAAAAATTTCATTTTTTATTTCTTAAATTTACCTTTCTTTTTACCTAGGCATTACCCCCTTATTAGCAGAAGTTTCTACTTGCTTTAACCAAAAGATTTCCTTTTTCTTCTTTAAAAATTCGCTTTACTTCGCCTAATATTTACCCTTTTTTATTGAAAAAATTCCCTGATTTTAGCCAAAAGATAGCTTTTTCTTTGTCTAAACTTTTAGCTCTTTTTTTACCCAAAAATTTCCTTTTTGCTTTTTTCTCCAAAATTTCTCTTTTTTTGAATTAGAAGAATGCACTTTTTTATTCAGAAGTTTTCTGCCTTTTGTCTTTTAAATAATTTCTTAATTTTTATCGCAAAACTCTCTGTTTTGTTTCCTGATAAATTCTTAGTTTTTATCTAAAAGTTTCCACTTTCTCTTTTAAGAATTTCTCTACTTTTTATCAAGAAAGTTTCTTTCTCTTGTTTTTCTCTAAAAATTCCACTTCTTATTCCACTTATTTTCAGTTTTAATTTCTCCTTTTATTTTTGAAATAATTTCCTTCTTTTATCAAGTTCTTAACCTTGTTCTTATTTGTTAATTCCCCTTCTTTTAGCCTACAACTTCTCTTTTTTCTTGTTAAAAGATTCCCTTTCTTTTTATCAAGAAAATCTCTTTTTTTATGTCTTAAAAGTGGTCTTTATTTTGGTTCAAAATTTAGTCCAAAAACAGTCAAATTTTTAGCACAAATTTAACTGCAAAAACTGTTCAAAAAATCTCACAAAATTGCAATCATTTTTTTGCAAGAAAATTTATCCCAGTTTTAGGGGGCAAGCGGGGTCAAAAATTGCTACAATAAATACCGTTTTTTCTTTAAAAATTAAGTAGTTAAATTGAACGAACGTTTTTTAATTGAAATCGAAGGTTTAAACAAAACTTTCGATGATGGTTTTGTATCAGTACGTGATATTAACCTCAAAATTAAGAAGGGCGAATTTATTACGATTCTCGGTCCTTCTGGTTGTGGCAAAACAACTACTCTTAGGTTGTTAGCAGGTTTTGAAGATCCTACTTATGGCAAGATCAAAGTTAATGGTCTTGACATTAAAGACTTGCCCATTCATAAGCGTCCCTTTGCTACTGTCTTCCAAGACTATGCCTTGTTTTCACACTTAACTGTGTACAAGAACATTGCTTATGGCTTGAAGGCAATGTACACAAAGCTGGATCCGATTGATAAGCTAGTGGAACAATACCACCAAAGCTTACTGGACAAGCAACACCGCTTGCACAAGAGGATTGAACGCTTAGAAAAAAGTAATGCTAATCCACAACTGTTAGAACAACTTAAACAAACGGTTGTCGTTCAACAAAAACAGTTTAAACAACAAACAGAGACTTTTAAACAAAAGGAAAATGCTCGACGTGATGCCATTCAACAACGCTTGGTGCAACTTACCAAGGAATGGGAAAGCTTATCAAAGCAGAAGTTGCAACAATTAGAAGCGGAGAAAAAGTTGTTAGATAAAAAGTTTGAACAAACTGAGCGCAAATACCAAAAGGATGCTTGGATGGCAACCCACAGTGAAATGCGTTTAAAGCAGTTCAAACAAGAGGTGTTAGCTTTAAAACAACTAATTAAAACTAAGTTCAAACAAAATGAACCAGTTGACAAGTTGCAGTTAAAGCTGCAAACCTTAAAGCAAAAGTATGCTGCTAAAAGACAAATTGATAAGGAATACGATAAGTTGGTCTTAGCCTACAACAAGAAGGACTATTGAACTTCATACTGGGAAACCTATTCTTTACAACAACAAGAAGCCTTTGAAAAGCGTTACTTATCGCGCAAACTCACTAAACAAGAACAACACCAAAAGGTGTGTGCTGTAATTGAGTTAGTTGGTTTAAAGGGTAACGAGGACAAACTCCCAGAAGAATTGTCTGGGGGAATGAAGCAACGGGTAGCTTTAGCGCGTTCCTTGGTAATTGAACCAGACATTCTCTTATTGGATGAACCCTTGTCTGCTTTAGATGCTAAGGTACGTAAAAACTTGCAAAAGGAATTGCAAAAGATTCACCAACAAAGTGGTTTAACCTTTATCTTAGTTACTCACGACCAGGAAGAAGCCTTGGTCTTGTCAAACCGCATTGTGGTAATGAATGAGGGTAACATTCTCCAAGTTGGTAGTCCGGCTGATATTTACGATTCACCCAAAACCGAGTGGATTGCTAACTTCATTGGTCAAGCTAATATCTTTAAGGGTACTTACCTAGGTGACCTTAAGATTAAACTGCACAGCGGTGAAGTGATTAAAACTGATGTGGACAATAACTATGTGGTTGGTAAGGAATACAAAATCCTAATTCGTCCCGAAGACTTTGACATTGTGCCCAAAAACAAAGGCTTCTTTAATGTTCGGGTAATTGATAAAACCTACAAAGGATTACTCTGAAAGATTACTACTAAGTTAGTTGATGACACCATAGTAGATCTAGAGAGTGTCAACGACATTGAGGTTGACAAAACCTTTGGGGTCACCTTTGATCCGATTGATGTTCACCTAATGGAGGTATAGGATGAAACTATCGAAAAAATACCTCTTAGCTGTACCATTCTTTGTTTTAATGGTCATCTTCTTTGTAGTACCAATGGCATGGATTATTGTTAGTGGACTCCAAAATGAAAACGGTGCAAGTATAACGGAAAAGTACCAACCCTTAGTTGGTGGTTATTCTTTCTTTCAGAGTTTTTGAACTAGTCTGTGAACGGCGACTGTGACAGTACTAGTAGCCTTACTGGTTGCCTTTCCCTTTTGTTACTTTTTATCACAAAGTAAAAACAAGGTATTCCGCAGTTTTGTGATTGCCTTAGCTACTGCTCCCATATGGAGTAGTTTTCTCATTAAGTTAATTGGGTTAAAAACACTGTTGGACTTAGTTTTGGGGTTAGCGTTGAACCGGGTGGGTGATAATAACCTCACCTTTGGTAGTGGTTATACCTTGATTGGGATGATCTACCTGTTCACCCCCTTCATGTTTTTACCACTTTACAACAACTTTTGTATCTTACCGAAAAACTTAATCTTAGCTAGTCAAGATTTGGGTTACAACTGGATTACGAGTTTTATTAAGGTAGTTATTCCCTTTTCTAAAACTGCCATTCTGTCAGGGATTGCGTTAACTTTCTTTCCGTCTTTAACCTCGGTAGCGATTGCCCAGTTCTTGGACAACTCGAATCAAAATAACACCTTAGGTAACTATGTCTTTACTTTAGGTAACAATGGTTATGACAGTGCGATTGAGCGCGGCCGTGCTTCTGGTGCCATTATTATTGCGGCTTTAATTACCTTTGCCTTTTACTTTGTGGTGATCTTTGCTCCAAGAATAGTGCGCTTAATCCAGACTAAGTGTTTGAAATACAGGAGGGTTAATGTTTAAGATGAAAAGCTGTAAGTTATGACTACGGGGAAGTTTCTTTGTCATTGTCCTTGTTCTCATCTACCTCCCTTTAATAATAGTTGTCTTAGTTAGTTTTAACGGTTCTTCGACTAGGGGTAACATTGTCTTAGACTTTGGTAATGTTTTAAACCCTAACCCGGATGCCAAGTCAGCATATCTAAGATTAGGGGAAGCGGACTTTGCCATCCCTTTACTCAACTCTGTCATTATTGGCTTAATTACGGTCATTGTCTCGATCCCGATTGCCATCATGACTGCCTTTGCTTTATTGCGATCCAGGCAATGGCTCAATAAAACAGTCTTTGGGATTGCTAACTTTTCTCTAGCAACTCCCGATATTATTACCGGGATATCGCTAGTGTTGTTATTTGCCAACACCTGGTTGAGCTTTAACCAACAGTTAGGTTTCTTTACTATTATTAGTTCCCACATCTCCTTTTCGGTGCCTTATGCCTTGGTGTTAATTTACCCAAAGATGCAAAAGTTAAACCGTAACTTAATCCTAGCGAGTCAGGATTTGGGTTACTCACCCATTGCTACCTTCTTTCACATTACCTTACCTTACTTACTACCGAGTATCTTGTCAGCCATTCTAGTAGTGTTTGCCACGAGCTTTGATGACTATGTGATTACTTCCTTAGTTCAAGGTTCAGTTAAAACAGTCGCCAGTGAGCTCTATTCCTTTAGAAAGGGGATTAAGGCCTGAGCTATTGCCTTTGGTACCATTTTAATCTTGGTCAGCATTCTAGCTGTCTTGCTAGTAACACTGCACAAGTACCTACGCTTTAAACATAAAGAAATGCTCCGTGTTAAACAATGAAAAAACAGTTAAAGTATTGGTTTAGTCTAATGGGACTAACCATGTCAGTTGGGTTAACTGCTTGTAGTTCCAACCAGTTTGTGTTTGCTAACTTTGAGTCGTATGTGTCGCCCTTACTTTTAGAAAGGGCAGAAGCCAAGCAACCAATGACCTTTCTTACTTATCCTACTAACGAAAAGTTAATTAACGGATTTGCTAACAATACCTATACGGTAGCAGTGGCCTCGAGTTATGCTGTGAGTGAACTGCAGCAACAAGGCCACTTACTACCAATTGATTGAGCTAAATTCAATTTAAAGAAAACCCAAAATGGTAGTAACCAAGCCACCATTCAAAACAAAGAGGATGCCAAAGAATTATTTACCAAAGAAATAGGCGACATTAGTGGTGAACTTTTAAACTGGGGTGTGCCCTACTTTTTACAAGACCTCGTGTTTGTTTACCGGGGCGAAAAGATTCAAGAACTTGAAGGTCAAGATGTTACTTGATCAACGATTATTAAAGCTATTGTTAATCACAAAGACCGCTTTAACAACAACCGTTTAGCCTTAATTGATGATGCTAGAACAATCTTTTCCTTAGCGAACGTCGTGCACCATGAGGTTAAGAATACCACGGTAGATGTTAATCCTACAGGTAGCACCTTAAACTACTTTGGCAACGTTTACGAAAGTTTTGCAAATTTAGGTCTAAAACGTGATAACCTCAACACGCTCTTTGTCAATTCGGACTCCAACATTATTATTAATGAATTAGCTAATGGCAGAAGACAAGGTGGCATTGTTTACAATGGTGATGCCGTATATGCCGCTTTGGGCGGTGATCTCCGCGATGAAATTAATGAGAATAACTTGCCCAACGGCGATAACTTCCACATTGTGCAACCGAAACACTCGCCTGTGGCCCTAGACTTTCTCATTATTAACCAGCAACAAACTCACTTTAGAGATGCTGCACACCAGTTAATTTACCAGTTGGCCTTAGAAGGCGCTGATCAAACAGCAGAAGAATTATTAAAAACTGATGAAGAGAAAGGTACCAGTGATGAGGACTATTACACTTATGGTGCAATGCAGAATTTTAGTTACGTTAACTATGTTTCGCCTTTAAAGAATATTTCTGATGAAACCACTGGGATTGTGTTTAAGGAAAACAAACAAGCTGATACTAAGCAAGTTGTCAAACAACAAAGTCAATCAGAACAACAATCTGAATCAGCAGAAAAGGAAGAAACGGAACAAGATGATTTCTACACTGCTACTCTCAAAAGTTTGTTGAAAGCGGACAGTTTAGATGACAAAGCTAAGAAGTTAGTAGACACAATTAAAAAGACTTACAAGATCAAAAAAGCCGACAATATTAATTGGGCTAACTTAATCGAGAAACCAATTACCCCATTACAAAGGAGTAATTTAACATTATCGTGGCTTGACTTTAAGCAACGCTTTTGATAATGGAACAACCTTTGTGTATCTTGGGAATTGAAACCACTTGTGATGACACTAGCATAGGGGTAATCACTGAGAGTAAGGTACAAGCACACATTGTTTTATCATCCGCCAAATTACACGCCCAAACTGGTGGCGTAGTGCCCGAAGTTGCAGCTAGGAGTCACGAACAAAACTTGCTTAAAGCACTGCAACAATCAGGGGTTGTTTTAGAACAAATTACTCACATTGCTTATGCCGCTAATCCGGGATTACCCGGTTGTCTGCATGTGGGAGCTACCTTTGCGCGCAGTTTAAGCTTCTTATTGGACAAACCCTTATTGCCCATCAACCACCTTTATGCCCACATCTTCTCGGCCTTAATTGACCAAGATATTAATCAGTTAAAACTCCCCGCTTTAGGCTTAGTGGTGTCCGGGGGGCACACTGCCATTTACCTAATTAAGTCCTTGTTTGACTTGGAGTTAATTGCTGAGACTAGCGATGATGCGATTGGCGAAGTTTATGATAAGGTGGGCCGCGCTATGGGTTTTCCTTATCCAGCTGGACCCCAGTTAGATAGTTTGTTTCAACCGGAGTTAGTTAAGTCACATTATTTCTTTCGTCCTTCCACGAAGTGAACGAAATTTTCCTACTCGGGGTTGAAGTCACAGTGTTTTACCAAGATTAAACAGTTAAGGGAAAGGAAAGGCTTTAATCCCCAAACCCACGATTGGAATGAGTTTGCTTCTAACTTCCAGGCCACCATTATTGATCACTACATCAATCATGTCAAGGATGCCATTCAGCAACACCAACCGCAAATGTTGCTGTTAGGTGGTGGGGTAAGTGCTAATAAATACTTAAGAGAACAGGTAACACAGTTACAGCTGCCTTACTTAATTGCGCCGCTCAAGTACACGAGCGATAACGGGGCGATGATTGGTTTTTATGCTAATCTTTTAATTAATGGCAAAAACAATTAAACATCCCCGATGGGGTAGATATGTAGCAGAAGCAGTTGGTCGTGGTCACCCCGATAAGATCTGTGACCAGATTGCGGACAGTATCTTAGATGAATGCATTAAGCAAAGTCCGACCAGTCACGTTGCTTGTGAGGTCTTTGCTTCCAAAAATCTGATTATGGTGGGCGGGGAAATCCTTACCACTGGTTATGTTGATGTGGTCCAAACCGGTTGAAAGGTATTAAATCGGTTGGGTTACACCGAAAATGACTTTAGCTTTTTGAGTTGTATTAACTCCCAGTCCAGTGAAATTAACCAAGCTGTGCAAAGTAATGACGAAATAGGCGCGGGCGACCAGGGCATTACCGTTGGTTATGCTTGTAGTGAAACCGAGCAATTAATGCCCTTAGGGAGTATCGTAGCGCAAGCGCTGGTGCAACGCGCTGCTCGTATCATTGACCAATACCCCTTCATTAAACACGACATGAAGAGTCAGGTAGTGCTCAACTATACTGGTAACAAAGTTCAATGTGAATCAGTTTTAATGTCGGTACAACATACTCAGGACGTAAGTTTAGACCAACTGCGCCAAACAATTATCAACCAAGTGATCCTCCCCGTATTAACGGAGTACGGTTTAAATGATCCTAAAATCAAACACCTAGTCAATCCCGGGGGTTCGTTTGTCGTTGGGGGCCCGATGGCCGATACGGGGTTAACCGGACGCAAGATTATTGTGGACACCTATGGTCCGTACGCTAATCATGGTGGGGGGAGTTTTAGTGGTAAAGACCCTACCAAGGTCGACCGCACTGGAGCTTACTTTGCGCGTTTTATTGCTAAACACATTGTTAGTTTGGGTTGAGCGGAAGAGTGTGAAGTGAGTATTAGCTGGGTCTTTTCCCAACCATTACCACAATCAATTCAGGTCAAATGCTTTAACATTAATAAGGAATTTAGTGAACAATTAATAAACCAAGTTATAAGTCAATATTTCAATTGATCTGTTGCTAAAATTATTGCAAAGTTAAAACTCCTTGATCAAGTTGAGTACTTTAGGTATGCTGTTTACGGTCACTTTGGTCACCAAACAGCCCCTTGAGAACAACTAAGTGAACGTGACTCGCTGCAATGTTTAATCAAAAATTTCCAATAAAAGCTCCTAAAGCACCATTTTTTAAAAAGAAAACACCACCAGTACCACCAAAGAATCCTGTGGTAACCAGCACTACCCCAGAACTCAAGAAAACCAAAATTAAAAAAGTAAAGGAAGCAGGGGATACCAATCTTTGGGATAAGATTAAAGCAAAGGTAAAAAAACACCCTTACCTGTTTGCTGTTTTAGTGTCCTTTATTCTTTTAATTGCACTTTTAGGCTGATTATTTGCAATTCCGATTTCGCAAATTCCTTAAGCTGCAAAAAGATAAAAAAGCAAGGTTCGCTTAAAAACCTAAGCCACCAAGTCCACCAAAGCCACTAAAGGGATTTTTACCACCTTTCAGCATCCGGCCCATTTCTGCTGTTTTCGTTTGCAGTTTACTCCACTCCTTCATTAGCTTATTGAGTTCATCCATTTTACGTCCCGAACCCTTAATGATGCGTTGTTTTCGCGATGGGTCACGATTAATTAATTTGGGGTGCCGGCGTTCTTCACGCGTCATTGAGTTAATTAATACCTTTCACAGTTTAACCTTGGCTTCAATGCTCTCTACATGATCATCCGATACCGTTAAGTTACCCGGGAGCATCTTGACAATACTACTAACAGAACCCATTTGGTTCATCTGTTCCATGTAAAGCAACAGATCTTCCAGGTCCATCTTGCCCAAGAACATCTTGCTAACTGTTTTGGTTAAGCTCTGTTTGTCAAATACTTCTTCCGCTTTTTCCACTAAGGTCATCACATCCCCTAAACCCAAGATCCGACTGGCAATCCGTTCTGGGTGAAATTGTTCTAGACCTGTGAGTTTTTCTGATGTCCCCATTAGTTTAATCGGAACTTCCAACAAACTCGTTAAGGACAAAGCCGCTCCAGCGCGGGCATCACTGTCAAGCTTGGTAATAATAAAACCGGTGAGTTTTAAACGGTTATGAAACTCCTTGGCAACATTAATAATTTCCTGGCCACTTAAACCATCGACCACCATTAGGATTTCATCGGGGTGGAGTTCGTTTTTAATTGCTACCAACTCATCCATTAATTCCTGATTAGTTTGTAACCGTCCCGCCGTGTCACAGATTATTGCTTGACAACCGCTTTCCTTAAAGGTCTTAACGGCCTCCTTGGTGGTTTGGTCGGGGGCTTGGGTACCCTTGGCAAAGAAGACAGACTCGGTTTGTTCGGCTAGGGTCGCTAACTGGTCAATCGCAGCGGGGCGGTAGATGTCTAGTGCCACTAACATCGCTTTTTGTTGAAACTGTTTTTTCAGTCACACCGCTAACTTCCCACAGGTAGTGGTTTTACCTGAACCCTGTAAACCGACTACCATTACCTTAAGTGGACGTTTACTAGTGATTTCTTGGTTGGGTTGGCTCAAAATGTTGATTAACTCTTGTTTAATTACTTTGAGCAGTCACTTCTGTAAGTCCTGACCGGGTTCTACGGTTTGACCAACGGTTTGTTCGCGAATCGCCTTAATGAAGTTTTTGACCACCAACAGGTTCACGTCAGCGTCTAAAAGAGCAATCCGAATTTCCTTTAAAACGGCTTGGACATCGGTTTCACTGATCGTTTGCGCGTTGATCTTTTTTTGCATCGAGCGCATAACGATGCTCGAGATCATTGACTTAAACATGGTGCTTTAATTATTTATTTTTTTATTATTTCTACGTATAGATAAAGCTAGTTTCATGACACCGCACATAAACGCAAAAAAAGATGACATTGCTAAGGTTGTTTTAATGCCCGGTGATCCCTTACGTGCTAAGTGGATTGCGGAACAATTTATGGAAAAGCCCCGGCTCGTCAACGAAGTGCGAGGCATGTTAGCTTTTACCGGTCAGTATAAGGGTAAAACCATTACCATTATGGGCCATGGCATGGGGATTCCCTCAATTGGGATTTATTCTTATGAACTGATGAAGTTCTATGAGGTTAATACCATTATCCGAATTGGTAGTTGCGGGGCGTTGCAAGGTTCCTTAAACCTCCAGGACCTCATTATTGCCGCTAAGGCTTGGAGTGAGTCGATTTACGCTAACGACATGGGTGTGGAAGTGCCAGCAGACAAGATCTTAATGGCCTCCCCGCAACTAGTAGAGTTAGCTAAAAAGACCGCTAACCAACTTCAACTGGCCTTCCATGAGGGCTTGGTGTTCTGTGAGGATGCCTTCCACCAAATCAGGAAAGACGTGTTAAAGCTAGCACAAGAAAAACACGCTTTAGCAGTGGAAATGGAAGCGCATGCTCTGTACGCTAATGCCATGCTGCTCAACAAGCAAGCGTTAACGATGCTCACGGTGTCCGATTCTTTGGTAACCCATGCTGCTCTACCAGCGGAACAACGCCAAGCCACCTTTAAGAACATGGCTATCTTATCCTTGGAAATGGCTAGCCAACTCGTTTAAACAATGAAATTAGAATACAACCGCATTATTGACAGTACACTACTTAAAGCTGATACCTTACCCCACGAAATTGATGCTTTATGTGCTGATGCTCATAAGTACCAGTTTTACGCAGTTTGTGTCAATCCTAGCTATGTGCGGTATGCCAAAAATATCCTTAAGGGTACGGGGGTGAAACTGTGCACTGTCGTTGGTTTTCCCTTAGGACAAACAACACAACGCCAGAAGGTGTATGAAACTAAGATTGCCATTAAGGAAGGTGCGGATGAAATTGACATGGTAATGAACATTGCCGAGTTCAAAAAGCGCTGTGCCTGTGTCATTAGTGAAATAAGGGCAGTCAAAAAGGTTTGTGGTAAGCGAACGCTCAAAGTCATTATTGAAACGGCTTTGTTAAACCAGGACGAAATTCGTGATGCCGTTAATGTCTGTATTGATGGTAACGCTGACTTTGTCAAAACTTCTACCGGGTTTTCCATGCGTGGTGCATCATTGGAAGATATCACAATTATGCGCGAAGCTAGTGGTAATCTCATTAAGATCAAAGCATCGGGTGGGGTTCAAACAGCGCAGCAATTCCTTGACTTCTTTAATGCTGGAGTTAGTCGGATTGGCACTTCCAATGCGGTCAAGATTATGGAAGAATTGCACAAACTAGAAAGCCATGAACATCGTTAACCTTATTAGTAAAAAGCAACGCGGCAAGGCGTTAACGGAAACAGAAATTAACTGGTTTGTCCATTCCGTTAATAACAAAAGCTTGGCCGATTATCAGGTCAGTGCCTTTTTAATGGCAGTGTGATTTCAGGGGATGAATTCCAAGGAGTTGTTTTGTCTCACCAAAGCGATGGTGAAATCAGGAGAGAGCCTCCACTTTAACCACCACAGCAAGTTAAGTGTTGACAAACACTCCACTGGTGGTATTGGTGACAAGGTTTCCATTGCTTTAATTCCAATCTTAACTGCCTTAGATTACAGTGTCGCAAAACTGTCGGGTCGTGGTTTGGGCTACACTGGTGGCACAATTGACAAATTGGAAGCAGTGGGAGTAAAAACAGATTTTACACCAACAGAGGCGCAGAACTTACTAGACCAAAATGACTGCTTTATTATTGGTCAAAGTGAGGGCATTGCTCCCGTAGACAAGGTGTTGTATGCACTACGCGATACTACGGCAACTGTGGACAGTCTGCCCTTAATTGCTTCCAGTGTCATGTCGAAGAAGTTGGCAATTAACAACGACTACATCTTCATTGACCTTAAGTACGGTAAGGGCGCTTTCTGTAAAACGAAGACGATGGCTAAAGAGCTAGCGCAGTACATGTACAGTATTGCCAAGCAGTTTAAGCGCAAACTCTACATTAAACTAAGTGACATGAACCAGGTTTTGGGTAAAACGATTGGTAATGCGTTAGAGGTCTTGGAAGTAGTGCACTTCTTAAAACGGAATTGAACTGAAGTGGGTGCTGACTTTATTCAGTTGATGGAGCAAATAGTCACTGAAATCCTTATAGAAACAAAACGTGCACCAAACAAACGGGCAGCTGTAGCACTTTACCACGCTACATTAGAGGGCGAAAAACCATGGCAGCGTTTTTTAAAATTTATTGAGCTGCAAGGTAGTAGTTGGGAACGTTTTTTGGATTTAAAAGAGCTGTTTAATCCCCAGTATAAAGCGCCAGTGTTAGCATCTCAAAGTGGTACTTTGAGCTATACCAGTCCGGTGGATTTAGCCATGGTCTCGATTAGCTTAGGTGCTGGTCGAATGGTTAAAACAGATCTGATTGACCCAATGGCGGGGATTAAGTTAGTGAAACAAGCTAACGAAGTGGTAAAAGCTGGGGACACAGTCTTAGAACTTTACAGCTCCAAACCAATTACGCCTGCCCACATAGAAGCTGCGCAGCACACCATTATTATTAAGCAGTAAATAAACCTCAATGAAAGTTGATTTAGATTGAGTCCACCACAAGTTACAGGAGGTGGTCAATCATGCCTACACGCCTTTTTCAAAGTTTAAGGTAGCTTGTATGTTGGTGGCCAATAACCAGGCATTTTATGGAGTCAATATTGAAAACGCTTCTTACCCAGTAACCCTGTGTGCGGAACGTAGTGCGATTGCTAACATGGTCACTAGTATCGGCAAGGCCACAATTGATTATGTCTTTGTGTACTTTGACACTAAAACTCCAACCAATTCACCTTGTGGCATGTGTCGGCAAAACATCTTTGAGTTTGCCACTGATAAGACCCAGTTATTCTGCATTGAGAAGGACAAGAGCTTTAAACAATTTACTATTCCCGAAATTTTAAAGGGAGGTTTTCGATCATATGAACAGTAACGCATACTTGGAAGCTCAAAGGTGGCTAAGCCATCCGCGGGTAAAACCCAACTTAAAGGAGGTCATTACGGCTATGAGTGCTGAGGAAATCGAGCACTTCTTTAGCTTAAAGAAACCGAGTTTTGGCACTGCTGGGGTACGGGGCAAAATGGCACCGGGGTACCATGGGATGAATGTGTTTTCCTATGCTTACTTAACCCAGGGTTATGTCAACTACATCCAGTCGTTAAACCCAACTAAAAAACCATTACGCTTTTTAGTAGCCAGAGACACTAGAAAACACGGGGCATTGTTTAATGGCATTGTGTGTGATGTGATTACCTCTATGGGTCATGTGGTCTATATGTTTGATAACAATGAACCAACACCAACACCATTAGTATCTTATGTGATTAAGAAGTACCACTTTGATGGTGGGGTGAATGTCACTGCTAGTCATAATCCTAAAACCGATAATGGCTTTAAGATTTACGATGGTCATGGCGCGCAGTTACTAGACTTCCAAACTGATCAATTAATCGCAATGTTACCCCCAGTGGTAACAATGTTAGATTTCGAACCACGTGGTAACAACGAGCTGTTACACTTTTTGGATAACGAAGTGGTCTACAAAAACTACTTTGATGATCTTAAGGAAAGCCTAGTAGTGGACAACGACAGCTTTAAAAACCTCCCGGTGGTCTTTACCGGGTTACATGGTACTTCGGTGAAGTTGTTACCAAGGTTTTTAACCTATTTGGGTTACAGCAACATTATTAGTGTCCAACCACAAAACGTGTTTGACGCTAACTTTGCTAATGCTGACCACCTCAACCCCGAAAGTAAGGATACCTGAGAGTTAGCGCGACAGTATGCATCCAACACTAAGGCCAAGTTAATGATGGCAATTGATCCGGATGCTGATCGCTTTGCGATTGCCGAATGAAATCCGCAAACCCAAGACTGGCACTACTTCTCCGGGAACGAGTCAGGAGTTATGGTGGCTTACTATAAGCTCAAACACAAGCAGTTTAAGCGTCAACCTTACATTGTAACGACGGTAGTCTCTACTGATCTAGTGGATAAGATTGCCAAGAAGTACGGGGCTTTTGTCAAGCGCACAAATGTCGGCTTTAAGTTTATTGGTCAAGCTGTTAATCACTTCTCCAAAGACAATGAACTAGTAGTGGCCTTTGAAGAAGCGATTGGTATGATGGCTAGTGATGGTTTGAACCGCGAAAAGGATTCCTTTCAAGCAGCGGCCATCATGCTTGAAATTGCGCGCTACTGTCATAATAAAGGAATTAGCTTACTAGAGTTCTACCGTGGTGAGATCTTCGGTGAATTTGGTGATTACTATAACTGAACGGTACCACACACCATTCACGGAGTTAACTGGAAGGAAAAAATGGAACAGGTATTACACCAGTTAACCACAGCTACCATTAAAGAGGTTGTGGGACACAAGATTACCAAGATTAAAAACTATGTCGACATTAACCTAGTCGAATATGTCTTGGAAAACGGTAACTGGATTAAGTTCCGCATTTCCGGTACAGAACCGAAGTTAAAGCTGTACTTTAACCTATCTAACGGTTACTTAGCAGCTTTAAAACACGAAGCGAAAAAGATGCACGAGTTCCTAGTGCGATTGCTCAACCTCGATAAAGCTTAGGTTTTCTAAAGGCAAGTTAATTAACTGCGTTTTGGTGGTTAATTCGACTTCCACTACCGCTTTGTTAATTGTTCCTTGGTATAGAGCCTTAATTTGACCTTGAATGTGAATCGCACCAGTTAAGTGGACTTCCACTAAGTTACCAACACTAAATTGTTGCAGTTGATCGACCTTAGTGTCACCTTCGACAATTTCGTATTTACCCAAGTAAGGAATTAACACTGATGGGTCAACCTTGTTAGCTTCACTTTTAACAATCGCTACATCCTCGTTTCATTCGTCTTGTTCGGCCTTATTAGCTTCCATTAGAGCTTGCGCAAACTGGTTAGTATCAATGATTTCACCAGATTCGTTAACTTGGGCAATTGCTTCGGGTTTAACTTGTTTGTGCTTGAGAATGTACTGACACTTTTCGTCAAACTTGTCCGCATCCATTTCCACAATGGCAGTGTTGGTCACTAACACCCGCTTTTTCGGATTGACCGAAATACCCTTAAGCATTTGTTCGAGGTTATCAGCATCGGCTTGGTCTAAAGGAGTTGGTAACGCACCGCGACCAGAGGAACCAACAATTCCAGTAATACCAGGGAAGTTACGGATAATGCGTCATGCATCTTCACTGTAGATCATCTTAATGTAGATGTAACGACCTAGTAAATTCTTCTCGCTAATCTTCACCTTTAAATAACGGTCTTCATCTAACACAAATCACTTTGTAAAAGCGGTGTTCTTTAATGAACGGGGCAGCTTGCCGGATTTTAAGGAAAAGACTTCTTCGATGACTTCGCGTTCTTTCAAAACCCTTACATCTAAGATTTCGTTGTCAAATCCCAAAGCTTTTACCTTAGCTTTGAGGTTTCGCACAACTGCTTCATCCTTAACACTTACAGGGGCAACGTATCATTGCGGGATTAATTCCACTTGTTCCATAAACTAATTATTTACCTTTAAAAACTCCAAGCGCAATAAAGAGGTGGTTAATGCCAAAGTAGATCCCTATCATAATGGCACTAACTAAAACAATTAACAAAAAGATCGTAATTAATTTCTTGCTTGTTACCCAGACAATCTTGTTAACCTCTTTGTCAATCCCAAACCACAGGTTCGAAAACTTCTTGGTAAAAGGCACCTTAACCTTTGGGGTTTGGTCCACTTTTTTATCTGTGTCTTTAGTTTTACTGTCTTGTTTCTCTTTGGCCTTCTTGGCTTCTATTTTGGCCTTTAGTTGACGGTGTAACTCGTGGATCTTCTCATCGTTGTAGGCGGTGAGTTTTTCCTTGTTCTTTAAACCAAAAGGGAGTTTCTTTTTTTCCATGAAGCTTAAAAGGAATCGAGGTGCTTGGTTTTTTGGTTGCAGTGCTTGCAATACTTATTAATTATAAGACGATTAAGAACCTGCTTCGACCAAGACATTACATAGTTCCTACTTAAACAGTCTTGGCAAACAAAGATAATCTTCTTGCGCACGTGGAGTTGGGTCAATTATATATTATCGGTTTGGGTTACCAATTTAATCTTATGGTAACAGTAAAGTTGGAGCAAACCAAAGGGCAGAAAACGTGATCATTTCAAGGCTTTAGCAAACTTTTGCTCGACGTTTTCGGCAACCTCGTTAATAATGTTGGCTGCCATATTGGACATAACTAATTGGGCCACCATTAAAAGGATTTCTAACACCCCTAAAACAATTAAGACTGTGTTGAGGTTCCGAAAAATCGGATCACGACGTGGTGCACTCAATGAAGTTCTGTTAATGAGGTCATACATAATGCCAATGCCAATTAGGGAGATAGTGCACACAAAGAAGAAGATGCACACCAGCATCGTTAGTTCATTGAGCAGATTGAGCTTCTTAATCACTTTCATGGAATAAGGTGTAGAGCTTATTTGATTTTAATTGCAATCGTTTAGCTAAATAATGACAAGCATCCTTAACCTTAACGCCCATTTGGACTAATTGGGTAATTTCTTGTTTTAAGAGTTGGTCGGAATATTGCCCCACCGGTTGGGTGTGGTGGTTGTTAATCACAATCACAAACTCGCCCTTTAGAGTAATGTCTGGCAGCGGTGCACTTGTATTAAACCAGTAGTGGCTTTCGTGGAGCTTGGTCAGTTCCCGCCCTACAAAAACTTCAGTATTACTAAACACCGTTTGGATTACTTCCAGTGTTTGTTGTAAACGGTGTACCGCTTCAAATAAAACAATGGTGCCTTGGAAGTTTTGGTAGTGTTTGAGTGTTTGGGTTACTTGAGTGGGTTTGTGGTCCAAGAAGCCCAAGAACAGTAGTGGACTTTCGGGGAAGCCACTGGTTACCAAACCACAAACCAGCGCACTAGGACCATTAATTACTTCTATTGCGATTTGGGGTAGTTGCGTTCTGACATACTCCACCAATATGTGCCCGGGATCGGATACTGAGGGATAACCCGCATCACTGACCAAACCACAGTGATACTGGTGGATTAAGTTAGCTACCTTACTTAAGTTTTGCTTTTCCTTAAACTCGTTGTTAATAATGAACTGTTTATTTGAAAAGTCAATGCCGAGTAAGCCCAAGAGTTTTTTGGTTACTCTGGTGTCCTCACAAAAGAGCACTTCACACTTGGTTAAAGCCGCTTTAACTCGCGGACTAACTTCCTCAATGTTGCCAATGGGGGTGGCAATTACTTTAAGAGCTGGCATACTTGGTCAAAGGTAAGTTGGAGTAAATTAAGTTTGCGAAAGAGCTGCTTGTGGTTAAAGTAGCTTAAGTGTAGTTTGTTACACAACGCTAACCGTTTACTTTTACTGTTGAGGTTTAATTCCAAATATTGTTCCCAGCTTAAAGTGGGTTGGGTTGTTTTAGTGAACACCTGACGTTGTTGTAACGCTTGGATTAAAGCTGCATCAGTTGCTTCCGCTACCCCAATTTTAGTGCTGTTGGGTTTCATGTCTTTCCGAGCTATAAAACAACTTTCATACTTATCTAAGTGTTGTTCTAACTGCCGGCGAATTTTCTGACCAGCAACATCGGGGTCCAAGAAGAGGATCACCGGTTGTTTTTTAGCTACAGCCTTAATCAGGTTAATGGTTCTTTGGTTTAAAGCTGAACCGTTCGTGGTAATAACACTAACATCAAACAGCTGTTGTAAGCGGGCTTGGTCAGTTTTGCCCTCACACACTATCACCCCATCTAGTTTTAGACGTGTTTTTTTATCCATTCAATGTGGCGCGTATAAATGGCAGAAATGGAAGAGTTATTAGCGTAACACAATAACACCTCTTCAAATAACGGTGCTAGATCAATTACCTGAAACTGGGGACACTGGTCAAACTTAGTTTGAGGAATGGAGTTGGAGACAAACAAATAGTCCACTAAGCCTTCGTTAAAGGCCTTTTGGAAGCGTTGCGGTGCTTCACCGTTAAACAAACCATGGGTGGCCATGACACAGACCTTTTTGGCATGGTGTTCCCGGAGTAGTTTAGCTGCTGCTATTACCGTACCACCGGTGTCAATCATGTCGTCCACTATTAGACAGTTTTTGTTCGCTACTTCACCCAAGACATTAATTGATTCCGCTACATTGTGGGCTGGGCGACGCTTGTCAATAATCGCTAGCGGTAGTTCCAAAGATGTTGCAATTAACCTTGCCCGCTTTACACCGCCATAATCGGGGGACACCACCACAAGGTCTTTTTTACCTAACAGCTCTACAGTGCGGGTTAAAAAGACGTGGTAGGTTCGCAATGTATCCACGGGTATATTAAAGAAACCCTGGGTTTGGTCGCTGTGGATGTCGGTTAAAGCTACGCGGCTCACCCCAGCCGTGGTTAACAAATCCGCTACTAATTTACTAGTTATCGGTTCGCGTCCCATTGTTTTGCGATCTTGCCGTGCATAACCATAGTACGGCAAAATGGCGGTAATACTTTTTGCACTACCCCGTTTTAAGGCATCAATTGCTATTAACAATTCCATTAAGGAATCGTTGACTGGCGCACAGGTAGACTGAAAGATAAAAACATCCTTATTGCGCACAGACTCCTCAAAGCGGATGTAGGTCTCCCCATCAGCAAACTCATTATGGGTAATTAACCCCATGGGCATTTTGAGTTTCTGACAAATACGACTAACTAAGTCGTGGGTTTTCGATAAGCTAAAAACAACGTGGTTATGGCGATCCATATTGTTGGCGCAATTCGCGCTGGATCGTTTTACTTTTGATGGCTTCACGTTTATCAAATTTTTTCTTAGGTTTGGCCAACCAGATTTCCATTTTAATTTTGCCATTTTTAAAGAACACTTTAAGTGGAATAATGGAAAGTCCTTCTTGCTTTTGGCGTGCCGTAATTTGTTTAATCTCATTCCGGTTTAAAAGGAGCTTTTTCACCCGTTTGAAGGGGTGGTTGAACTCGCCTGAAAAGGCATAGGGCGAAATGCTAAAATCTTTAATATAGACCTCGTGCTTGTCAATAAAAACATAGGCTTCCTTTAAGCTACCTTGCCCTAAAGCTAGCGATTTGACTTCACTACCTTTTAAGACTAAGCCAGCACAATAACCCGTTAAAAGGTAATAGTCATATTGCGCTCTGGGATTGTTGACAAGTACTCGCATCTGTAGATGAAAATCTCTGTTATTATCTCCACTTACAACTGTGGTGCTTTAATTGTGAAAGCACTATGTTCATTAGTGTCAAACCAAACACCAGCATGTGAGTTAGAAGTGCTTGTGATTGATGATGGATCCATTGATAATACTAGGCAAATTATCAAAAAATTTCAAGCAAAAGTTAGCTTTACTTTAAAGTACTTTTACAAAAAAAACGGAAATTGGGGTAGTGTAATTAACTATGTCAAGGAAAACCGTTTGGCCAACGGCGATTGGATTACTGTTTTAGACAGTGATGATACCTTAAAACCTAATACACTCAACAAGTTGGCTAATTTAGTGGAAAAAGCGGATTATGACTTAGTAGTTTTTGACTACACTAAGTGCTGAAAAAAGATTAAACTGAAAATCCACACTTACCCGACTTGGTGAAAAAACATGACCCGGGAACTACAAAAGCAAACACCGTTTTGTATTCCTCTAGGTAAGTTTTTGAAACGCAACCTGTTTTACAAGTTGCCCAAGCTCAAAGAAAAGGTGAGTTTTCAGGATGCCTTGTATACGGCTAGTAGTTTAAAGCTCGCTAAAAAAGTGCGTCATGTTAATCAGTCTGGTGGCAACTACCACTTTAAGCGTGCTGGTAATTCAATGAGTATTCCCTGAAACATTAAGCGCTTTAGTGCTGAATTGGACATTTGTAAAGACTTAATTCGTTTAAATGCTCAGGAAATTGCCCTAGTGCACTTATTGCGACAACAGTTTCGGGTGCAACTTAAAGAAAAGCAGATACAACTAGCGGTTACCCGTGACTTTAACTTTAGTGGGTTCTCTTGGTACACCCGCTGCTTTTTATGGATGGTATACCAAACGATGTTAAAGCGCTACTTTTACTTACAAACAACTAAACAATAAAAAAGGCTGTGCAGCCAAACCACACAACCCTTTTTATTTTCTTTACTTTAACTATTTCAACAAGTCCGCGTAACGGTTCTTTTCCTTACTTGCCACCAAGAACTGTCACTGGTTTAACTTCCACTTTTTGGTCATTTCACTTACCAGTTCATCGCCATTCTTAAGATACGAAGATGGCAGTGTTACCACAATCGCAATGGCTGCTAACAACAAACCAGCAAACAGCGCCACACCAGCAATTGCCCCTGGGAGGATGCTTACTCCTTTCATTCCAGGGTTTGCTAGTTTCGGTGCTTCTAAGAACATTGAGTTAGTAATGTCAAAGGCGGTGTAGATCACATAACCAAAGCCCCAGATGTAACCAAACAACACGGACACTTCACTACGCTTGTACTCTTTGAATTCGTGCGGGAGGATCAAAATGGTACTTTGCATACTCCAAGCAAACGAACCACCAATAAAGGTTGCAATCGCCACAAAGGCAAAGCCAACAGCAGTACCCACACCTAGAGTGGCTGCAAACAATACGATTACAATCACCATTAAGGCATTCATGAAGAACATGAAGCTCAATCAACGCTTGCGATCGTAAACCGTTTTGTTAAATGGTGAAAACACCATGTAACCCATACCATAACCTAATACCCAGAGGATTGCTAAGGTTGGGAGACCAGCAAAGTAACCACCAGCACCCTCTAGTGGTTTTAATGTTTTAACACCATCTTTAACATTAAACGAACTCGCTGGACTCACTGCTTGGAGAATGTTTCATCAACCACCAGTAAGTGGGTTTACTAACACCACCAAACAGATGCCATACATCCCGATCATCTTTCAGGTCGCTTTTCGCTTTAAGATATCACCAGCACTTGGTTGGTTACCCAACTGCATTTGTTCCTTGGTTTGCTTTTGGGGCATAAACGGATCCATTTGCTTTTCAAACCAAGCAAACACACAGAGAATGCCAAAACCAATCAAGATTAAAGCAGTTAGGATAAAGACCCAGTACTTAGTACCAGCTTGTTGAACAGATTGGAATAAGAACGGTACAAAAGCAACTACAATCCCACTGTTAAAGCCCCAGAGGTTCGCGTTGGATAACACTGACTTACGACGTTGCGTTGAAAGTGAAGCAATTAACGGTTGGGAGTAAGTTACTAAAGTTGTACCACCAATAGCAATCGTGGAACGGAAAATAATAAACAACGCATAACCAGCAATTGATGAACTGGTACCAATTAAATTTTTATCTAAACCTTGAACTAAATCAATAGTTTGACCATCTGCTAACATGGTCTTGCCTTCTGCCATAACAGGCCCCAACAATTGACCTTCATGTAATTGCGAAGATAGACTAGAAAGTTTACCCATTACTTCCTTTGAAAGTGGTCGCAATAAAGTTAACTGGTTATTTCCCCCTAATGGATCACCAATAATTAAGAAGGGGAAACAGAGACACATTAGCCCCATCATAATCATGACCGCATAACGGTAACCAAACTTCAGGACCACTATTCCACACAATACGGATCCGACTGCACGTAGCAGGGTAATACTCCAGTTGGTGGCACTACCAGCAATTGATCCAGCACTGTTAGCAAAGAAGGAATTGACCCAACCATCATAGTTAGGTAAAACCTTAGTATTGCTATCTAAAATACCAGCTGGCTTACCAGCAATTCGGTCAATTACAAATCACTCTACGACAAACAACAAATAACCAAGCAAGACAATTGCCCACAACATGACAAGGCGTAAATCACTCGGCTTTTTTACAGACGTTTGCACAATCTCAACGACTCCTTAGTTAATTTATTTTTAAATTGTTTCAAGTCGACTTTTGTCCCTTATCTAAAAACAAGCTAGTTTTTTAGGCTTATCTTTGTTAACAAAATAAAAGACCAGAGCCGTTTGGCCCTGGACTTTTATTCCTTTTAGCGGTTTATCTAAGTTATTTTAAGAGATCCGCGTAACGGTTTTTAGCTTTATTCGCAATCAAAAACTGTCACTGCGTTATTCTCCACTTCTTTGTCATTTCACTTACACATTCATCACCGTTTTTAATTCAGGATGATGGGAGGTAAATAATGACCCAGTTAATAACTAGAAGGAGTGATACAAACAAGACAATAGCGGCAATGGCACCTGGCAGAATATTGGCACTTGTATTACCAGTAGCAATTCTTGGTGCATCTAAAAAGACAGACGTAATAATGTCAAAGATGCTGTAGAAGACGTAAGCAAAGCCTCACACATAACCAAAGATAATAGAAACTTCGTTGCGCTTGTATTCCTTGTACTCGTAAGGGAGAATCAAGATCGAGCTACCTAAACTTCAACCAAAACCACCAGCAATAAAGGTAAAGATACCCACAAACGTAAAGCCAACCACAGTATTTAAACCAAGCGTTGCAGCAAAGATTACTATTAACAACACCAACACGGCGTTGGCCGTAAAGATAAAGTGGAGCCAACGCTTCTTGTCATAAATTGTTTTGTTAAACGGTGACAGTAAGAAGTAACCCAAGAGGTAACCCATTACCCAGAGAATTAATAAGATTGGACGGGCAGTTGGAATAATTGCTTTTTTAGCACCTGAAATAGCTAACAAGCTGTTAAACCAGGTTTGGGTTAACGGGTTAATTAAGAGAATTGTACCTACACCAGCTATTGCCAACAGCTTCCAAGTTGTTTTGCGTTTCAAAATTGATAACGCACTTGGTCTCACACTCAAGCTTTGATTGGTTTTTGATTGCTTTTGGGGCAACATGTGATCGACCTTCTTTTCAAATCAAGCAAATACTAACAACATCGCAAACACCAATAAGATCATGAACGTTACAACTCAAACCCAGTTAGCAGTCGCAAAGCGGCCAACACCTGGAATTAAGAAAGGTGCTGCCACAATTACAAGTCCTACGTTAAAACCTCAGAAGTTAGCATTCGATAAAATCGATTTCTTGCGGTTTGAAGAAAGGGAAGCAATAATTGGTTGCGCATAAGCAATTAAGGTCGTACCACCAATAGCAATCGTAGAACGGAAAATAATGAAAAACGCATAACCAGTCATTGACGCACTGGTACCAATTTCGTTGCCGTTAATTCCTTTAATTAAGCTAACAGGAGTGCCGTCAGCCAACATAGTTTGACTACCCACTTGAACAGGACCTAACAGTTGACCTTGTTGTAAACTGGAAGAAATTTTAGTAAGTTTACTTATTACTTCACTTGAAGCCGGTCGCAACAAGGTCAACTCGTTATGACCACCTAACGGACTACCGATAATTAAGAAAGGAAAACAAACACACATGAGTCCCATCATGATCATCACTGCATGACGGTAACCAAACTTCACCACCATTACCCCACATAAGATCGAACCAACTGCCCTCAATAAGGTAATAGTTCAGTTAGTAGCTTGACCTGGAATAAAACCAGCGTCCGCTACAAAAAAAGAACTGAACCAACCACCGTACTGGGGAACAGCACTAGTTTGTTCAGTTAAAAGGGAAGCCGCACTACCTCTAATGAAGTCAATCACAAACCATTCAATCACAAACAACACATAACCAAGTAAGACGAGACCTCACAGTGTAACGAGCCTCAGGTCGCTTGGTTTTTTTACAGAATTTTGCACTTCAGTAATAAAAGAAAACGCAAAAATTTTTATAAATTGTTTGCAACAGCAAATTCCGGAATTAAGCGAGCTCTTCTAAAAATTCCGTTTCTTTCTTGTTAACAAAAAGCGCAATAAAAAAGCAGACCACTGATGGTCTGCTTACAATTTATATTGAGAAATTTTTATGGCGGAAGCGGTGGGATTCGAACCCACGCACCGTAGAACGATCTAACACCTTAGCAGGGTGTCCTCTTAACCACTTGAGTACGCTCCCAGGAACTGGAATCTAAATTTATTATAAATATTGGATTTTCTGACTTTAGGATGTTCAAGCCATTGTTAAGCGCTGAGCTGTTTTTTAACTGAACAGCTAAAGATTTTAAAGATAAAACAAGCTTTTTAAAACAAGCGTGTCGTGTTTTACAGGACAAAAACTGCATTAAAGAAGAACAAATAGCACTCACTGCTTTAAAAGAAAGGGAGGCACAAATCACCACCGGAATTATGTCAAAACTGGCATTGCCCCACATGCAAAGTGCTACCGTGTTAAAACCGTTTGTGGCGGTGTTTAAGGTCAACAATGTGGACTGGCAATCCTTAGACAACCAACCGGTTAAACTAATCTTTTTAATTGGCGTACCCAAAGATCAAGGCAACCTCCACTTGGAATTTATTTCGCAGTTTTCCAAGTTAATGTTGCAGGACGAGTTTGCCAACAAAGTGCCCAACATTCGCAGCTTTAACGGGTTGATCAATTTAATTGACAGTTTTCAACAAACTGCAGTTGCAAGTCAACCTGTTGTTAACGAAGCAGCAGCTCAGACTGAAGAGCCAAAGGACACAAACACTCAGTATGACTTTGTGGCGGTAACGGCGTGTCCCACCGGGATTGCTCACACCTTTATGGCCAAGGAAGCGCTGGAAAAGTTTGCCCGCGATCACAACCTCAAAGTTAAGGTCGAAACTCAAGGAACTGATGGGATTCAGAACCAGTTAACCGAGTCTGATTTAAACAATACCAAAGGCATTATTTTGGCCTGTGACCGCTTAATTGATTTAACGCGTTTTTACGGTCACGCAAATGTGGTGGAAGTGTCCACAACCAAAGCAATTAAAACACCACAAACAGTTTATGACCAAGTGGTAAAGAAGGAAGGCAAACTACTCGGTAATAAGTCCAGTGATAGTGCTAGTCAAACGGAACTCAAAGAAACCACTGAACAGCTCTCGTTTAAAGACTTTCACAAACGGATTTACCGCGCCATTTTAAGTGGGGTTTCTTACATGCTGCCATTTGTCGTGTTTGGTGGCATCTTAATTGCAATTGCCTTTTTAATTGACATTAATAATGCTGGCAATGCTGGTAAACAATTTGGCTCTAAAGATCCCATAGCTAACTGGTTTAAAACACTCGGTGGGTTGTCGTTTGGTTTAATTGTTCCCATTTTAAGTGCTTACATTGCCTTTGCTCTAGTCGGCCGGCAAGGGTTACTGCCCGGCTTTATTGTGGGGTTAATTTCAGCTGGTAAGTTTTTACTCAACATTGACATTGTTACTGGAAAAATTGATTGAGCGACTGAATCAAAAGTATCGAGTGGTTTCTTTGGTGCCATTTTTGGAGGCTTGTTGGCAGCAGTGTTAATTATTGTGCAGCAACGTTACATCTATAGAAAATTACCGCAAGCATTACAAGGCATTAAGAACATTCTCTTTATTCCGTTACTAGGAACGCTAGTCACAGCAGCCTTGTTCTGAGTCATTAACATTCCCCTAATTTACTTGAACTATGGTCTGTCCAAGTTCTTGCAAATTATGGACAAACCATATTTGGCTCCCTTACTTGGTTTAGTGATTGGACTCATGATGTGCTTTGACTTGGGTGGACCGGTCAACAAAGCGGCCTATGTGTTTGGGGTAGTTTCGCTAGAGAGCCAAAACAGTGGTACGGTTGCAATGGCTAGTGCTATCTTATCGGGGATGGTGCCACCATTGGGTATTGCCATTGCTGCGACCATTCGCAAGCAGTGCTTTGACAAGGAAGAATTACCAGCAGCGTATGCTTGTTATGTGATGGGATTAAGTTTTATTAGTGAAGGGGCGATTCCTTTTGTTGCTAAACGGCCTAAGATTATGTTAGCTGCTAACCTAATTGGTGGTGCTGTGTGTGGTGTTTTAACGGGAGCATTTGCGCTAACGATTCGTGCCCCTCATGGTGGCGTGTTTGTCTTTGCCTTATTGAAAACTAACTTAGAGGGCATTGCCGGCAACACACTCCAAATCGGCGCTGGGGTTGGCTTAGCACTTCTAGCGCTAATAGTATCCAGCTTCATTAGTGCTGGCATTATTATTGGTCATAACCTGCTTGTAGTGCGTAAAAAAACAAAACAACTAGTAAATACCAATGCTTAACCACAACAGTAAAGTTTGAATAGTTAACTATGCTTGTGCGATTGACTATTACTTAGATAAACACAAACAACAACGCGGTGTGTTAACACCCGGTGGTAAGGGAATTAACATGGCGATTGTAATGGCTTTATTTGGCATCAAACCAACCGTGTTAACCTTTTTGGGTCAACCTACTAAAGATTTATTCTTGCAGTTGTTAAAACCGTACCAACTGGACTTAGTTAGCTTTCCAGCGACTACCCAAACACGGATTAACGTGAAGCTGTTGGATGGTGCCCAAACAACTGAAATTAACGATGTCACCCCGTTAATTGAGGAACAAGCAGTCCATGAAATGATTGCTTACCTTAAGGCTAACGTTAAGCCCAATGACCTGTTGGTGTTGAACGGCCGTTTTTTACAACGCGATTTAGTTAAGTTGTTAGATGTTGCTTTTAGCTTAACCAAGTATGTTGTATTAGATGTTGATGAACCTCAACTATTACAACTGTTAAACCAGCGCCAACCCTGGTTAATGAAACCGAACCGTGATGAGTTTGTAGCAATGGTTAATGCAAATAACAGTAACGTTGACCAACAAGAGTTGGTGCAGTTAATAAAGCAGTTTCAGACTACACAGAACTTATTAATGTCAGACGGCGCGCAGGGTGCTTACTTCTTTGATCAACAGCAACTGTTATTTATGGAAGCGATTCCTCCCCAACAGTTAGTTTCCACTACGGGAGCGGGAGATACATTGCTGGGTGTTTTCCTAGCTAACTTACTGTTAGATAAGGATCCAGTGGGTAGTTTAAAGGTAGCGGTTAACTATGCTAGTGCTACGATTAGCAAGCTCGCTGTGGTCAACAGTAATGACCAAATAGTGCTAAAAGCTACTAATTATTACTATTTATAATTAGTAGATGTCAACTAAAGAACTAGCCAAACAGCCAAAGGTCAATCAAGAGAGGGAATCGATTTTGTTACCCGTTTTGCTAGCTGTTTTCAGTATGCTCTTCTTTATGCTACTGTTAGCTTTAGTGTTGTTGTACGCTATTCCAGGTAAAGTTAATAACTTGAACTAGCCACAGGTTTAACTGTGTTCAATTGGGAAGCCAAACTTAATCTCGCACTTTTAATGAACTAGTTTTTGTGCGCAACTTAATTAAAAACGTTTTTCGCTCTTTTCGCACCGCTAAGATTGCCTTAATTGCGCTTACCTTTTTAATCTTTGTCGCGGTTGGTAGTTTCGTGTTATTAAACAATACCGTTAATAACTTTAACGCTTCCTTTCAACACGTTACCGAACAAGGACAATTGTCTAACGCCATTATTAACGAGCGCTATGACTTTGGTAAACTCGAGTTCCAAAAAACGAGTAATGATGCTACTAGTTCTGCCCAACCTAGTGTTAGTACTCGAAGTACTTTAACTGCCCAAAAAGCAAATGGCGCTAATTCCAGTTTTACTCTGACCTTAACACCTGACTCACGGACTTCATTTATTAACAACGCACTGCAACTAAAACCGGATGTTTATAACAGTTTAGTTACCGTTACTTTTAGTTATGGCAGTGAATCTGATCAAACCAAGAAAACCCAAATAGAAGAACAGTCCAAGTTAATTGCCGCAAACAATTTAAGCCGGGCATTACAAACCGATAAGGAGTTATTAGTTACGGGACAGTTAGAACAGTTAAAGGCTACCTTTCGCACTTATAAGGCAATTAACATTACTGATCAAGGAGTGTTCAAGAAGCTCATAGCCTCTGAACCGACCGATCAAGTTAACCGTCTCGTTCTGTTTGACGGACATCAACTAGCACGTTCCCGCCAAGTCGAATTTAACGAGCTGTTTAGTCAGTTTACCCAGGTGCAAGCTAAGGGTAAAGATCAGTTATCCACTACTCTAAAGAATGGTAATTACGAACAACTTTTGCAAGCGATCGCTAACAACTTTGGTAGTAGTGAAACTGAGTTACTTAAAGAAATTCAAGATGCTTTAAAGCAAACGAATAGTTCCACCACCCTTGCCCAAACCCAAAGTAGTCAACAATTTAGTAAGTTAAAAAACCTGTTTGATTCTGGTAGTACCTTTACTAACATTGCCGGCAAACTCACGTTGCAGTGGATGGAAGGTACAAACAAAAAGCAACTGGTAATCTTTGACCCGAGTAGCTATGAAACGGTAGTAGCACCGGGTAACTGGAGTTACGAACAGCAACAGGGCAAAGCGGTGTATCAGGACATTAACCACTGAAACCGGATTAAGCGTTTACCCTTCAATGAGTTTGAACGCGAATTCCAAAAGATTGATAAACGGTACAAAATGTTGGTCGACAAAATTGAATACCTCATCTTGGGAGTGGGAATTACCCCAGACTTTGTCTACCCCGTCTTTAGTGAATCACTGTTAATTCCCAATACGGCTAACGAGCAGTTGTATTATGTCAACCAAACTGGTTATGAACGTACCGCTAGCTCCTTTTTAACTAATCCCACCGAAACTGGCATAGTAGCACGGTTACCTAACCTCAACCATGACCTTGATGTAATTAACCAGTGGGCTGTTAAAAACATGTCCTGACCCCCGAACCTCAAGGCCGCTTACAGTAATACCGATACCACTAACATCCTTAACCTGTTAGCAGCGCGAACCGTTTTTATTCCTAACCTCATTAACACGATTAGCTTAGTCTCTTCCTTCTTAACAATTGCCATCTTACTAGTGGCCATCTTTGTCACCATTTTAATCTTGGTTAGTTACCTCAAAAAGAACACGGAACAAATCGGCATCTTAAAGGCTAATGGGGTGAGTGGCCGGAAGATTAACTTAAGCCTGTTAATCTTTAGTTTAATCCCGGGGATAGTGGGGTCCTTTATTGGTTATACGTTAGGTATTAGTATGCAAAACGTGGCCATTAACCTCTTTAGTAACTACTGGTTTATCCCCACGCAAACTTCGAGTTTCTCGGTGTTAGGGTTACTTTTCTTTGCCTTATTAGTGGTTCTTATGATGAGTGGGATTAGTCTGTTTGTGGGGTGACTGATCTTAAGGCAGGATGTAGTCAAGATTCTTAAACACGACAGTGAGTTTAAGGTATCGCACTTAGGGTTGTGAGCTAAATGACTGTTTGCTAAGTTTGGTATTATGACCAGATTTCGTGTCGCTTTAGCCTTTAATGCGCCGTGGAAACTGGTCTTTTTAACCTTAATGAGTGCACTAACGATGATGATCCTAAACCTTAGTTTTGCCACTAAGGATGCCTTTCAGGTAGCACAAACAAAGACTACTTTAACCCAATCGAACCACCAGTACGAGTTTGAACTAGCATCGCCAACGGAGCAGAGTGGCTTGCTCAAGTGACAGCTGTTTGCCGAATTGGGCGCTACGGATGATCGTACTACCGATGCCGTTAAATTAGCAAACCGAAGGATGGAAATTCAAAGCGTTGACGAATCCAAAAAGTGAAAAAACCAACAGGTAATTAACTTTGTTACTGATGCCACCGGTTTTAGTAATGACATTAACTATTTAGAAAACATTGTTCAGTCCAAAATTGGCTTGGACTATTCACTGGGTTTTGGTAACGTTTCTTCCAATCCGTGGAAGCTGAGTGAAACATTAATGCCCGCCAATCAAGCATCCGCTTCCAACACGGCTTTCCAAAACTTTTTAAAGGCGATTATTGTAATTGACCCTAACGAGGGCGGTAAGTACATTAAACAAACTAAGGATGAAGTTACTGTGCGTTTTTTATACGAAATAGACAGTAGTAAAGCTTTAAAAAACGGCAACACCAAATCAGTTAATAAAATACAAAAAAGTACCGCAGCACCCCAAGCCCAACAAGGACAAAGTAACCAGTTAATGCTTGACAATGATTTTGCTAAATTCTTGTACAAGCAGTTTAACCTAATCAAGGAAGGGGGCAAAGCAGATCCGGTTGATCTCAATAACTTTGACTTTGCTAATCAGAATCAGATTAAGCGTTTTTACAGTAAATACAACGCCTTACCACCGGTAGATTACAAGCTGGCCTTTAACGTGATCGGTCTCCCTAAAGAAACAATTGCCAACCAAAAGGATGCGCCCCTGTTTGGCTTTTTAACCTTGCAAGCGCAACTGGGCAATGACGCGATTAAGATTAAGGGGATTAAAGACTGAAAGCTGGATAACACCAACTTGGGTCCTGTGTTGCGTACCAATGAGGACGAGATTATTAACCAGCGCTTGTTTAGTAAACCCAACGGTATTATTATCAACAGTTCAGCTGCTAAAAAGTACCGCTTAAAGGAAGGTAGTCAGCTCCATGTCAAAATTCACAACGGTTACAAACGAGTGAATGCTAAGTTAGTTAACCAAGATCCCACTTTAACTGCCACCTTTAACATTGTTGGCATTAACAATTCGGTGCACGAACCGGAGTTTTACACGAGTTACAAAACGGCAGCAACACTATTGCAATACCCCAATGAATGGATCGAAAAGCAGTTACCCTTTAACAGCTTTTATGCCAATTCGATCTTGCCCTTTACCCAGTCTACTTCCCTCTTTTCCGAATCTGGCTTATTCCCTGGTACCAGTAGCTTTGCCGCTAACAACACGGTGCTTACTGAAGTAATTAAGAAAACGATTGAAGGTGGTAAAACTAACAGTAAAACTGATGTGCAAAAGCGCGCTGCTCAAGCTACTGCTAACGGTAAGTATAACAGTAGTCAAAACAACAAGATGAATTATGAGCGCTTACAAAAGGCACTGGGCATTACCAGTGATTTAGATAGCTCTAAAGCCGGTGAATACGCCACCATCTTAGAGCGGGTGTATGATGGTTTGCCATACAACTCCACTATTAGCTTTATTAGTAATGTGCAGGCCAACGATGCCTTGTTTGCTAACATTGCTAACACCACCCAGCAAATCCAAACAGCGGCCATTGGCATTATTATTCCGATTATTATGCTAATTGTGTTGTTAGTATCAACTACCCTAATTCAGGAATTGAAGAAGATTGCGATTCGTTTAAAAGCATTGGGCTATTCCAACCCGAAGATCTTGCTGTCCTTCTTGTCGATTTACCTCCCGTTATTTATCTTTGGTTTGTTAATTTCGATCCCAATTTCGATTTATCTAATTGCACTCCACAATCAAGTTATTTTCTCCAGTGCTGCCATCTTACTGGAAGCCACATTAAACTTCCCCACGGTCATTCTGTCGATGTTAGTGTTAAGTGGGGTGTTATCAATTACCTTTGTGTTGAATTGGTTGGAGTTGAACAAAATCAAAATTGCCAAGGAGATTAAGAATAGCTAATGCGATACTTTGCCCTCACTGATTTAACCAAGCCCACTGCAAAATTTACCACTGATGCCATGGAACTGCGTCAGATATCCAATGCTTACCTCAACCAAGCCCAAGCATATTTGCAAAAGGGTTTAAAGCAACTCAAAAAGGATTACAAGAATGCCATTTTGTATACCCCCAAAACGGAGTACAAGCGCTTTTTGAAATGGAAGCAAACTTTTCTTCAAGATTTAAACCAAACCCAAAAGCGCTTCTTTATTGTTCGTGCCCAACATTTTTCTTACGTGTTGTTGTTTAACTTATTGGATGAACAGGTGGAAGCGGTGATTGCGACCTTTAACAACTTCTTGGATGAACACCGCTTGGAGGCACAATCCAAGCAGTTTGATTTAGACACAGCTGTCAATGAACTGCATGATTACTTTGACCAACTGCAAAAGAACACCGCTTACAGCGAGGATTTACCAACTCACCTCACGCAAAAAACCGAGCAATTAATTAACGCTCGTAATACCCAGTTAACGAATTTGTTAAACAAGATTGCTACTACCAAACCACCCCTAAACAAACAGCAACGTTTGTTAGCCAGTTTTCGCAATTACCACGAACACCTCTTTTTAAAGAACGAGGTCAAAAAGGTGACTTGGTTAAACGAACCACGCGCCAAAAAAGAGTCTGTTACCCCTGATGAAGAACACATTATTGAACTTAAAAACGTTTACAAGTACATTACTAATGGGGTGACTACCAATGCCGTTTTAAAGGGCATAGATCTCAAACTCAAAGCCCATGATTTCATTGTGATTTTAGGTCCCTCTGGCTCGGGGAAAACGACCCTGCTCAACATTATTTCCGGGATGGACCGGCCATCAAGCGGATCTGTTGTCGTTAATGGTCAGGAAATGATTTGCATGAACGACCGTCAGTTAACTAACTTTCGCCGCAACTATGTCGGTTACATCTTCCAGCAGTACGGGCTTTTACCCAACCTAACTGTAAGGGAAAACGTTGAAGTGGGTGCTAACTTGCAACGTAACCCTGATAAGCGGATTAACATTGATGAACTGTTAGAAGCAGTAGGGATGAAACACCTCCAAAAGAAACTCCCCAACGAGTTGAGTGGTGGACAACAACAACGGGTCTCGATTGCACGGGCGTTTGCTAAAAATCCCTTACTCATTTTTGGTGATGAACCAACTGGGGCTTTGGATTTGGAAATGACCCAAATTGTGCTCAAACAGTTTTTAGCCATTAAACAACGCTATAAAACCACGATGGTCATAGTGACGCACAATAACCTAATTGCCCAACTAGCCGATTTAGTCATTTATGTGGCAGATGGCAAAATTCAAGCACTGCAAGCAAACCCTAATCCCAAACAGGTTGAGGACATTAACTGAATTTAGATCTAATAAAATTAGAGCAAATGAAAAACCTGTTTGCTTGCCAACACTTAGCGTTAAGTGCTATTCAACACGCTAAGGGTGGTCATGTGGGAATGGCCTTAGGTGCTAGTCCTATTTTGTACACCTTATGGACTAAACACATCCAGTTTAACCCTAACTGTCCCAAATGGATTAACCGGGACCGTTTAGTGATGAGTGCAGGTCATGGTAGTATGGCCTTATACCCGATCTTACACTTTGCGGGGTTAATTACGAAGCAGGAAATGCTCCATCACAAATACGGGCAAGTCAACACCTCTTCCCATCCCGAGTATGCCCCTAATAACTTTATTGATGCGTCCACTGGTCCCTTAGGACAAGGCCTAGGAATGGCTGTCGGGATGGCCTTGACACAACGGGTACTAGCAGCTGAATTTAAAGCGTTAAGTCCCAAACTGTTTGACCACTTTACCTATGTGGTTGTGGGTGATGGTGATCTCCAGGAAGGGGTGAGTTATGAGGTAGCTCACTTAGCAGGGGTCTACCAGTTAAATAAGCTAATAGTGCTCCATGATTCCAACCGGGTGCAGATGGACAGTGTCGTGCGTGATGTGTCCTTGGAAAACCTGCAAACCCGGTTTACCAACATGGGGTGAAATTACCTTGAAACTAGTGATGCAGTTGCAGACATTGATGCTGCCATTAAACAAGCGAAAAAGTCCGATAAGCCGACCTTCATTGAGGTGCACACGACAATTGCTAAAAACACCACCTTGGAAGACCAACCCGCTGGTCACTGGTTTATTCCTACCGACAAGGACTTTGCGCGCTTTAACAGCAACACCAAGACTAACTTTACCCCGTTTGAATATCCCCAAACGGTGTATGACTTCTTTCACAAGCAGGTCATAGCACGCCAAGCTAAACCAGTGCAAGCTTACAAGGAATTACTGGAAAAACTAAAGGACAAACCGCTGTATACTAAGTTTATTAATTGAACGGAAAACGATTACCAGGCGTTGTACCTCAACCAGTTAGATGAACGCAAGGTAGCACAAGCCAATGCGGCAACCCGTAACTACCTCAAGGACTTTTTAGGCCAAATTAACAACAGTAATTCCAACTTGTACTGTCTCAATGCTGATGTAGCACGTTCGTGTAACATTAAGTTAGGTGATGATAATTTACATACCAATCCGCATTCGCGCAACATTCAGGTGGGTATTCGGGAATTTGGTATGTCCACCATTATGAACGGGATGGCACTCCATGGTGGGGTCAAAGTGATGGGTGGTACCTTCCTAGCGTTTGCTGATTATTCCAAACCCGCAATTCGCTTAGGGGCGTTAATGAACCTCCCCACCTTTTATGTGTACACCCATGACTCTTACCAAGTCGGTGGTGATGGACCAACCCACCAACCGTACGATCAACTCCCGATGTTAAGGGCCATTGAAAACGTTCAGGTTTGACGGCCTTGTGATGAAAAGGAAACAGCGGCTGGGGTTAACTATGGTTTGTTAAGCCAAGACCAAACTAACGTATTAATTTTGACCCGTCAAGCTTTGCCTTCCCTAGAACAATCTGATAGTGTACAGACCTTGAAAGGGGGCTATATTATTAGTAATAGAAAACAACCCGATGTCATTGTGGCCGCTAGTGGCAGTGAAGTTCAGCTGGCATTGCAACTAGAACAAGCTCTAAACGAACAACAATTAAAGACGCGGGTGGTGTCAGTGCCCAACATTAACATGTTGTTAAGTCAACCCCAAAGCTACTTGCAACAACTGTTTGATCCTAACAGTGTTTTACTCACCTTAGAAGCGAGTGCTAGCATGGAGTGGTATGCACTAGCAAAATACGTCAAAAAGCACACCCACTTAGGTGCTTTTAGCTTTGGTGAGTCCAATGACGGGCAAGTCGTGTATGAACACAAGGGCTTTAATGTGACTAACTTACTCAAGTTGATTAAAACTCTAAAGAGTTAAAATAGAGTAATGGTTAAGGTCTGAAAAATTGGGTTTGGGGTCTTTCTACCAACAGCGCTTTTATTCTCGGCCTGTTCGTTTAAGGACTATATTCCTACACCAAGCTTTAGGAAGGATTTCAGTACGGAGAACAATTTTGTTAAAAATAAGGTTCCCGGGAAGGATGACATTTACAGTAAATTTTACGATCTAACCTTTTCCTTAAACTTTGTCAACAACCAAGCCCAAGAGTTTGGTACCGGTTGGTTAATTGACTGGAAGGGTGATGAGAATAAGAATCTAAGCAAAAACAAAGAAGGACAAACGGCATCTCAAACCAGAAGTTCCTCTGAACAAACTACCGATCAAGACGCTAACCTCTTTACTGCCTATATCGCCACTAACCTCCATGTCGCGGATGGTTTAAAGAATGATCAGGACTACGCTCCTTACAACAAGGATGGTTGGGGTCAACCATATCCTTACCAGCAAAAAACCCAATCCTTCTTATTAGGTAAGTACACCAAACCTAATGTCCAGTTAGTCAAGACTAACTATGAAAAGCCAGAGGATGCCGTGATTGAGCAGAAGCTCAAGGAAGATTCGCTGTTGTTTATCCAAACGAGTACGTTACCTAAAACAGCATACGCAGCAATTGATCCGGTGAACTTTAGCTATAACCCAACCCGTACCAACGGTTTTTGAACAGCCGGTAAATACAATGTTTACAATGGTGGTAACAGCATTGGTAACTATGCTGATTTTGCTGTGATTGAAGTACCGTTAGTACTATCCAACCCCAATGATGCCAAGATCTACCAAGAGTGGATTAGACCAGCTACCCAAGCCTACAAATATTTAGGTGATGTAGAAGGTTTGTTTGCTAAGAAGGGCTATCGCAGTTACATCCAAGATTTCTACCACTTATTGGGTTATCCGGTAACTAAGAACACTAAATCAGAGTTTATTTTGGGACAAAGTCAAGGTACTGTGAACCACATGAGTTTTAGTAATGATGAGTCCAATACTACTAATACTATTACTAAAGCTAGCTTAACTCAGCAACCCCACAAGGAACAATCAGCTTACGTGGTACGTGAAAGTGGACTGCCAACCCTAACAATGAATGTGGACAAGTACACTGGTGCAAAGGGAACTCACCTAGTTAACGTTGACCAAATTACTGACCTTAGTTTGGGTGATGGTTTAATTGACTTTGGTGGATTATCACGCTTTATCTTGCAGTACCACAATGTGAACTATAAGCAATTTGGTTATGGCACGATCTTGTGAGATACTAACTTTGCCGGTGGTTCTTCTGGTAGTGCCATCTTTAACCAAAACAAGCAGATTAACAGTATTTACTTTGGAGCATTAGTTAATGTAACTACCGACAGAAACGAAAATGTGGGTTTAGGCTTAGGGCAAATTCTCCGTGCTCCCAATACCTTCAACTCTTCCCACGAAGTGCCCTATTCTTATGACCTCATCTTTGGTGATGTTAACACCACCAATTTTTACGCCCAGTTTGCGAAAAAGCACAACACCCACATGTGGAGCAAAATTCAAAGTACGCAGAATGGAGAAATTGGTTTCCACAAAAATTCAAAAACCGGTCAACAGCGGCATTAATCGGTTTTTGAGTAAATTTATAAAGTACTATTTTCTTTAACTAGTTGCCATGTTACTCCGCTCTGTTTGGTATAAGCTTGGTAGTCTGTTAATCATCTTACCGCTAACGGGTTGTGGTTATGTCAGACTAAAGCGTGCTAATTTCCAAACAGACTTTACCATTAACCGCATTCCCACCCAAGGGGATGTGTACCATGATAACTATGACCTGACTTTTTCACTCAACTTTGCTACTAGTTCCAAAGATTCTTATGGTACGGGTTGGTTAATTGACTGGAAGGGTGATGAGAATAAGCCTAGCAATACCGATCCCTTCCTGATTTACTTAGCGACTAACCTACACGTAGTCGATGCCTTGCGCAATCCCCAAGACTATGAACCTTACAATAAGGATAGTAATGGTCAGGACTATGGTAATCGTGATATAACCCATTTCTTTGCATTGGGTAAATACACTGATCCAGGTTTATTGGGTGTTGAAACAAAAGAGCAATCAGCCTTTATTTCGATTCAAACATCAGCAATCCCCAAAACCGCTTATACGGCTAATGATTTTGTTGATTATCAGTATGATAGTCTTACAAAACAGTGAAATAAAAAGAGTGATCAAAAAGATCAAACTCAAGAACAAACGGTAATAGGGCAAAGCTGATCTTACAAACCAGCTTATGCTGACTTTGCGGTAATTGAAGTGCCATTGTTCTTAGACAACGTCCGTGACAAACAGGTCTTTGATTATTTTGTCAAACCAGCCATTGAGACCTATAAAAAGCTTGGTGATACGGCTCAATTATTTACTGAGCAAAACCTAAAGGAATTGGAAAAGGAAACCTATATTATGCTGGGCTATCCGGTAGTGGAGAGCAATATTTATGCCCACATTTTAGGTCAAGGAAAAGAGTTGCGTGTGACTCAACAAGTCAACAACCAACCAGTTAAAAAGAACTATGTTTTGCAAACCATTACTAAAGAGCAACACACTTTGGATATTACGCGTGAAATACCAACCTTAATCCAAAACAAGAGTTTATCGGGTGATTTTGTTGGTAGTAAGCTCTTATCTCAAGAAGAACAACAGCAAGAACATGCTTTTTTGGGTAACTTAAACCAAGGGATAATTGACTTTGCTAGATTGTCAAACTTTAACTTGCAGTACCACAACCGCGAATACCAACAATACGGTAAGGGTTTAGCATTAGCTAACACTAACTTTAGTGGTGGTTCATCCGGTACCTTAGTTTTAAATCAACAAAAACAAATTTCCGGGGTTTACTTTGGCGTGTTAGAGTTTGGTGGCACCAACGGCACTAACCGTGAAAGTAGTATTGGTGTTGGTCAAATTTTGCGAGTAAAAGATGACGCTCAACTCCAACAACACAGTCACAACAACTTACTTTCACAATTAGGTTCCAGTCATAACTCGATTACTTACGACATTATTTTTGGTAACAAAGACACTAAAAACTACTACGCCCAGTTTGCCAAAAAACATCAAACACATTTGTACAGCCAAATAAGTTCAAGTAATCAGCAAGAACTCAAATACGTCGATAACGATCCAAATCTCAAGATTAAAGAAGAGGCTGCCAAAAGCACTGTTCAGAATTTAACTGTCTACAGTTAATTACTTTTCGCATTTCAATAATTGAAGAGTTGGTTAGCACAAATTTAAATTTAAGGCTAGTTTAATGACTATTTATTTTTAATTAGTCAACAGTGATTTAAATTTTATGCAATTAACTACAAGTCAACAAATGCAAACTAACTTTCTTACTACAAATAAACATCACTTATTGAAATACACCAACGGTTTGATTTGGTGTTGGTGATTATTTGTTATTTCTTTAGTTTTAGCTTCATCGACATTTAGAGGTTTCTTTCTCGGCACTATCAATATTGTGAATTTTGTGTTTTGGATATTAGCACTGATTTTTGGTGTAGCTGTCGCTTTCATTAATGGTGTTTTGAGTAGTGAATTAAAAGAAAATTCAGTTTTTCAAGAAGAACAAAAACGATTCTTCTTGGGCTTTTTCTTTCCACAAATGGCTTTCTGTAATGCGCTTTGACTGAAACTTAAATTAAGCTATCTAAATTCTGAAAGAGAAAACTTATTAGAAAAAATAAAGCAAAAACTAAAGAAGCTTACCTTGTCTGTTTTTGTTGTTTGAGGAATTTATTGTGTTTTAGCAACCTCAATTTATTTACCAAATGCATTAAGAATTTTAAACATTTATCAAATACCTAACTTAATCGCTTTAATAAATAATAGATTGAGTGAAATTTTGCCAGATGGTAACCGTTATTTTTTAGGTCATTCTTCATTCGCTTTTCATTACTATGAAATAGTTTCAAGGATTCCTTTTTTAGTTTTCTTTATTATTCCAACAATAACCTTAATTACTTTAGGTTGCTATTTATTCGCTTACTTAAGGTTTATAAACTCAAATAAGTTAAGAAAACCACTTTCTACTTTATCAATAGTTATCATGCTGACTGATGTTGTGGGAATAATTCAATGAATAATTATTGATATCTTATTAATTTGGCTTAATGTACCCTTTGTTATATTTGTAATTTTTTGAGTAATAAAACTTGTTTTGCCTTTAGCAATGATAGGTACGTTTGTCAGCTCCTTAACTATTTACAAAAAAGTAACAAGCAAAGAATGATTAGCAATAAAAGAAGAACAAATTAATTTAACGACAATGAATATTAATATTAATATGGGAGAGCAATCATCCAAAAACATGAATTCTTTTGAAAATCATGAATCAAATGAACGAAATAGTCTTCAAATTTATCAACAACATTCATCTATGATGTCAGAAACAAAGAGAAAACAAAGTAGCTTAAGTTATGATGCCAGAATCCTTTTACCTAAAAGTCCCTATAACACAAAGAAAACTTTATTTTTAATTATTTTCTTTTCCATTATCTCCTTGATATTGGCAACTATAGGTAGTGTGTTCATTTCTTTTGCTATAGTTCAAATTTCTATACCTTTTTACGTTATAGGTGGAGTTATTTGATTTTTTACTTTTATTTCTTTATAGTAACTTTTGTAGCAATTGTAAGGTTCATAGCAGAGTACTTCGCTAATAAGTTAAATGCACTTACATGAAAAGTGGATCTTTCTGGACTAGAGATTAAACCTAAGGAAGAAGAATAGTTATTGATTAAATTTGTTATTAAAAACCAAAAATTTAGTTTTTAAATAACTTTCTGTCTCTAAAAAAACGTTATCTTGTAAACTCAAAGATAGTTTCAGATTAGCTTTGCTTGCAAAAGTTTAATCTGTAAAAAGTTTTAATTCTATGGAGTCTGCACTAAACCAAGAGTTTCAAATTGATTTTTGTGTAAAAAACAAAAAACTGTTAAAAATTTTAGCTAACGTTCTTATTGCTTCTTGGTTATCGTTTGTTGTTTTTTTAATCCTAGGTTGTATAGCTATTGATCTTTTTCGATTTGATCTTTATTCCCAATTTTTCTTTAATCACTTAAGTACTTTATCTGCTTTAGCATGAACGTTTTTTGTATTAGCAATACTTTTTGGAGCAGCTACATTAGCCATTAATGGGTTTTTCTATAAAGAAATAAGAAAAAAATCAGCTTTTAAAGAAGATTCTAAATAGTTTCTTTTAGGCATTTTCTTTCCCCAACTTGCTTTTTCCAATGCGCTTTTCCTTAAATTTAAGTTAGCCCATTTGGATTTAAAAAAAGAACTTTTATTTAAAAAAATAAAGCATAAAATAAAACTAATTAGTTTATTTATTTTTGGATTTTGATCAATTTATGTTGTTTGCATGACTTTAATTTATGTGCCAACTACACTTAATTTAATTGATAGTTTCAATTATTCCGAATCCATCTATAAATGAGGTGACTATTTCTTTCGCCATTTAGAAAGTAGAGATTTTTATTTTAGTAACTTTGGTTTTATATCACTCTTTTTGTTGCTTTTTGTTATTCCAACAATCACTCTCACAACATTAGGTTGCTTTCTATTTAGTTATTTACGGTTCACAGATATAAACAAAATAAAGATACAGATTTACAGCTTGTTAACTGTTTTCATTTTTATTGATGTTTTTGGTTTAGTGGTGTCTGTTCTGTTTGGTTATCTTTTACCTCTTGCTTTTGACTCACTGCCTTTTTCAGTGAATTTAACAAGAGAGGTGTTTTTGTCTTTAGCTATGATAGTCATATTTGCTAACAGTGTCATTTTTACTTTAAGGCAAAAAAGAAATATTGATTAATTAGCGAAAAAGAACGATTAAAGCTTCAAGCAATTGCTAATTTAAATATGGAAAAAGAAACAAAGAAAGATTTATTAAGTGAAGTGAAAAATAGCTTTCATTCTTCTAACAAAAATATTAAAAGCGAAATTATTTTGAAGAACACTTCAGGAAATAATATTTATTTAAGAAGCGATGCGAGAAGGCTTTTACCTAGAATCCCTTATAACACAAAGAGTGCATTATTATTCACCCTTTTATTTGCATTTCTATCTGGCCTTTCTTATGGTCTTGCATCTTTATGTATTAACTTCTCGTTTACTTCGCCACAAATTTTCATTGTCTTAATTTATTAGGGGTTCTTTTCTTTATTGTTGGTCTTGTTTTGGGAATTATTACCTTTATATGATTAATAAGATTCATAGCAGAATACTTTGCTAATCGTTTTTATGCACTTAAATGAAAAACCGATGTTCCTAGTACGTAAAATAAATCGAAGCAATAATTTAAAAATTGTTATTAAAACAGAATTTTAGTTTTTATCTAGATTTCCGTTTCCAAAAAAATGTTTCCTTGTAAATTTAAAGAAGTACTTTAAATAGAAAAAAGTGAAACAGAATTTAAGTAGTAGAAACTTTATTAAAAAGTCAGAAAAAACAGTATCAACTACTGTAAATATAAATACAGAAGCTAGTAGTTATATTGCCAAAAATCTAGAAAACCAAAAGGCAGTTCTTTCAGCAAAGGAATTAGAAAAACAAGAGCTTTTACTAGTGCGCTTAAATGCAGTAAAAAGATTTCCGTGCTATCCATACAATACAAAAAAGGCATTTAATATTTCTGTTTTATTTGTTCTATTAGCTGGTCTATTTTTTACTGCGTCTTATTTCTCTATTTTTTATTCGATTTTTACTAGGTTTAAAGCTTTACTTATTTTCTTAGGAGTAGTTTTTATTCTTATAACTTTCATTATGCTAATTCATTGCTATATATGGTCAGTAAGGTTCAAATCAGAGTACCTTGCTAATCGTATTTATGCACTGAATTTTCAAAAAGGAGCCTAATATAAGTGTTCAACAAAACCCAACAAAAGAAAACGTTAGTGATGTTAGCATGATAGAAATAAATAATTTGACCGCTTTAGAAAGGGATGAAAAAGTAGAAACGGAAGACATTAATACCAATGTAATAGCAAATACTTATACAGTGCAAGCCAATGTTCCAACTGCATTTCAAACCAGTGAAACAAATAGTAATACTGTTACTAACGTCCCAAAATCTCCAGAGTTAATAAGTATTCAAAGATTAGAAAGATTAAAAAATAGGTACACTTTCCTATAATTTTTTTCAATTTTAATTGGCTTCATTTTAGCCATATTACACATTGGAGGCTTTACTTTTGCAGGTGGTATTAATTTATTGGCCTTTTTAAGTTTTATAGATGCAGTTGCTTTTATTTGTTTTATTGTTTTCCTGGTTTTAGCAATTTCTACATCGCGAAGAATTAGAGACTTAGAAATGATGCAACAAATGAAAGAACTTGTTGATAAAAAGCAAGAGACACCAGAGCAAAATAAACAGTAAGTTATTTATTTTTAAGGGTTATTTATTTCTCTTTTTAGGAAATCCATTTGAAATATTTTGCGAATTTAGTTTATTCAATTCAAAAGACTTTTAAAAGAAATTAAACTGTTACTGTCGTGGAGATTAAAACTTACAAAATTAAAGATATTTGTGAAGCTTCACATGGAAGAGAAATAAATACTAAATATCTCCGTGAAAATCAAGGAATTTATCCTGTTTATTCATCAGCAACTTCTAATGAAGGTGAAATGGGCAGAATCAAAACTTACGATTTTGATGGTGAATATGTTACCTGAACTACACGTTGAAGTTATGCCGGTAGCATTTACTATCGAAATGGTAAATTTAGTGCTTCCTCAAATTGTGGAATTTTAAAAGTTTTAAATAAGGAAATAAATCCTAAATTTTTAGCTTATGCTTTAAAAAAAGAAGCAAAAAAATTTGTTAATACAACTTCTGCTATTCCTATCCTTAGAACGCAAAAAGTTGTCGAAATTCCCATTGATTTCCCACCCTTGCAAATTCAAGAAAAAATTGCTACTATCTTAGATACTTTTACCGAGCTAAGCGCCGAGCTAAGCGCCGAGCTAAGCGCCGAGCTAAGAGAGCGAAAGAAGCAGTATGCCTTCTATCGCGATTATTTACTTAACCTAAAAAATTGAAAAGAGGAAAATAAATATTACAAGCTGGGAGAAATAGCTCAAAAAGTTTTAGTAGGTGGTGAAAAGCCTGCTGATTTTAGTAAGGAAAAGAACGAAGTTTATAAATATCCAATTCTTTCTAACAACAGTAAAGCTGAGGAATTTTTGGTTTACAGTAAAACTTTTCGAGTTGAAGAAAAATCCATTACGGTAAGTGCTAGAGGCACAATTGGTGCCGTTTTCTATCGTGATTTTGCTTATTTGCCTGCTGTTAGTTTAATTTGCTTTGTACCTAAAGAAGAATTTGATATAAGGTTTTTATTCCACGCATTAAGAGCCATTAAATTTAAAAAGCAAGGTTCTGCAACAGGCCAGTTAACAGTGGCACAATTTAAGGAGTATGGTATTCATGTACCTTCGCTGAAAAAACAGAAAGAAATCGCTGCTATCTTAGATCCACTTTACAGCTTTTTTACTGATCTAAACGAAGGTATTCCTGCTGAAATTGAACTGCGCAAAAAGCAATTGGATTACTATCAGAACTTCTTATTTAATTGAGTGCAAAACCAAAAAGCAGCATCAATCCTGTAAGCAAAGAACTTTTTCTCTATGTTCTTTTAGTGCATTTTCTAACTGAAAATATCCAAGTAATTAGTTGTATTTTTCAGAAAGCTTAACTAAAATTCCATTGATTCGGTAGTTTGTTTATTTAAGTATTTGGCCAACATGACCAAATAAATTTTTTATGCAAACTATCTTAACTGATCAAGCTAGCTTCAACATATTAACTAGCAAGAGAAATTTAATTAATAAATTAACTATTACAAACATAGTGCTTTGAGCACTGTTTGTCTTTTGTGCAATAGCCACAGCCTTTTTAGCTATCAGCCATTCTTTTCACAAGAATTTTTCTTTTTTTCTGGAGTCTGGAAATTCAAACAACGAAGGTGAAACAACAACGAAAGTTAATATTCACGTTCTTGTAGCTCAAATATTAACCCCCATTTTTACAGTTTTACTGATTCTTTGTTGAATGGGCAACATCGGGATTAACAGTTTATTGTTCGGTCAACTAAGTATTTGCCAACAATTTCAAAATGAGAAAAAATGATTATTGTGAGCCATCTTTATCCCACAACTCACACTAACTAACTCACTGTTAGTAAGAGAAAAACTGAAGAACATTTTGGATGCAAATAGCAATAGTTCTACTCAAAAGAACAGACTTACTTGATGGATGATTGGCTTTTTTGTTGTTTGATTTATCCAACTTTTTCTAGGTTTCTTAATTTATTTACCAGCTTACAGTGCTGAAGCTCGCTTAAATTTAAATTTATCTTTTTTATCCTTTGCCTTAAATTCACCTTTACCAGTAGGGCACGCAGCCAATGGCTATTTTTTCTTAGGTTTGATTTTTGCATTTATGGATTTAACCATAATAGTACTCCTAGTTATTAGTGGCTTCATCTTGAAGCATTTAATTCAAACTAATAAAACTGAGCACAAAAAATACCAACTAATGTTTTTCTGAACTGCTTTAGCAATTGATGTAATTGGGCTAATTATGACCATTTCATTTATAGTTGTTAGCTTTTCCTTAGAAAAAGGAGATGACTACTTATTAAAAACACCTTTCTTTGGTAGTTCATTGATGGCATTAATTACTATTGCAACGCTTGTAACTACAATCCTTTTATTTATTAGATTTAATAAAAAAAGATTACTTAAAGATTAATAAATAGTTTCCAACACCTACCTCCTCCAAGACCACAACACCCTCACCCCCTACACGCCCTTTACGACACCGCTCAATGGGGGGCTGGATGTTGTGCGCGCCGCCCATTTACACCCCTCATACGAACTCGTGGACTGAAAGGTGGGACAGAACAAGTTGGTGGCGCTGGTCCGCTCAGCGTTGGTCAGGGTGAAGTTTCAAGATAGTTCGAGCCAACAAGGCACTGAGAGTAATGGCCAAGACCAAAATGCCCTTAGTTTTGATACCACCAAGTCCCAACAAGCCTTACAGCAAGCTGGCGATCAATCAGGAACTTCATCTAATTCCACCCAAGAAGACTTCGCCAGCTACATCCTCATCTTTAAAGCCGCGCCCAGGGCCACGTGGGTGTTTGAACGCAAGATTAAGTTGGCGTTGCCCTACGTTAAGCAGGAAAGTCAGGGTTCCGACGATCAAGGTTCCAATGGTAAGGGCTCCCTCTACACCACCTTAAAAGACCTCCTCGTCGAACAACCCGTGACCCCTTACACGCCCAATGCGGGGTTAGCCCGGGTGAATGGGGTTGCTCAGGACAAGGTGCATGGGTTGGAGTCTGGGTGAGACAACCAACGTTCCCAAAAAAACCTCACCAACAACCCCGGACCCAAAGCCGTCACCGGCTTTAAGCTCGATAAGGGCCGCGCGTACCGGAAGCTGAATGAAAGTTGACCGGTGTCGAACCCCTCGATTCGACCAAGGCTGGCAAGGGGAAAAGTTCCACAGAATGAAAAACGGAGGAGACAAATGCTAAGAGTGATGCTCCCCTAGCTAATGGTCAGCAAGTGGGGGGCTGGAAGTGCTTTTGGTTTACAAGGCAATGGCTCGAACAGTTCGGGGTTAAGACCGCTCTTGAAGCGCACCGCCCAAATTAATTTAAGGCAAACCCAAGACAACGCGCAAACTGGTAAATTCTCCAAATACCTCAACACCGCCCAGGCCTTGCACCAGATGGGGGTGATAGTGCCATCTTTGGAAACGTGACCTGGTAAACCAAGTACTGGAATCGCTACCCGTGCTGTTGGTGGTGTTTCAGTCCAAGCAGCGACTCGGCTCGACTTCTACAAATGAAGATCTGCCGAATGTAATAACAAAGTTATACCACACCTCCACGTCCCAACTCGCTTACTTAAATGGCCAGATCGTTGTGATGGGTTCAAACGCGGTACCGAGTTTGTGGTATTGGGTTGTCGGGGAGGACCAGGAATCGGGTCGCGCAACCTGATGAGCCCACACCGAGCTCAACTGGGGCACCGACAAGCAGAAGCAGTTTGTCGAAAACCAGTTGGGTTTTAAAGATGACTCCAATTCGGATTCCAAGAATTCGAATTTGAAGGCTCAAGGCCTCACCCAACCCGCCTACCTCATCGCCGGTCTTGACGTTGTGGCCGACCACCTCGTCTTTGCGGCCTTTAAAGCGGGCGCGGTGGGGTATGATATGAGCACGGAAAACAGTGCTGCCACCAAAGACCAAGCACTCGCCTGGTCGACCACGGCCGGGTTGGACAGTGCTGGGGGGTACAAGGCCTTGGTGGAAAACACGGCCGGGCTCAACGGTCCGATTAATGGCTTGTTTACCCTGCTCGACAGCTTTGCCTATGTGACCCCGGTGAGTGGCATGAAAGGGGGTAGTCAGAATAACGAAGAAGTGCAGACCAAGTATCCCGTTAAGGATGATAGTAAGGCTTCCGCCAAAATTGCGTCCTTAATTAATGCCAGCCCACTCAACAGTTATGGGGATGATGGCGTGACCGTGTTTGATGCCCTGGGCCTTAACTTTAACTTTAAGTTGAACGAGGCGCGCTTGCCGTCGCGCACCGACCAACTGCTTGTGTATGGGATTGTAAACGAAAGTGAACTGAAGTCCGCGCGCGAAAATGCCCAATCAACTTCCGACGCTAATTCGAACACCAAAGTCAAGTGAACCAACACCGCCTCGCACTACCTCCCCGTGCCGTATTACTATAGTGCCAATTTCCCCGAAGTCGGTAACAGAAGAAGAGCGGAGCAGCGGAATGGGGTGATAACAATAAAAAGGCCATCTACCCAGAGGTAAGAGGCAGACAGCTCCAAAATATTTTTGACGATTGGAAAACTAACAAAAGCTGTTAGTTTGATGTAGAGGCAAAACTATGAATTTACTAAATTTATTTAAATCAGCGGGAGCAGGCTCGTTTTTTTAACACAAGTCACTCTATCTGCTCCCACTAAATAAATTGAGACAGATTTTCAAAATAGCCAAAAAGTTTGAATATTTTGCAATTTGGGTTTACAACTCCATCTAACTATTAGTAGGAACTTTTACTTTCAAAAAAACTTTTTGGTTTATTTTTGGTGTCTTTACTAACTTAAAGCAATTTAGTTAGTGTAAGCACAAAAACCGTTATGTGTAATGTTTTTATTAATTTAGCAAAACAGCTGAACTTAGTTGGCAGCACTACTGCAAGTTGTAGTTTTCTCAACCTCTTTCAGAAAGGAGTTATTGCTTAATGAAAGAGAAAATTCCGTTTTATAACGAAAAAGAATTCCATGACATGATGAAAAAGACCAAAAAGGGCACTTTTTCGGGATGATACATCATTGACAAAGATAACAAAAGTGTGGAATTCTCTGGCAATTTCAACAGACAATTTAAACTTAACAAACCGGTTATACCAGTGAACACAGAATACGTAACGCGTAAAGAGTTTAATGAGTACAAAGACTCAAACGACCAACGACTTACAAAGATTGAAACCACTTTGGCCGCCCAAGGTGAACAAATAAGAATCCAGGGTGAGCAAATTAAAGAACTTCAAATCGAGCAAAAAGCTCAGGGAGAAACCTTAAAGCTAATCTTGCAAACGCTGCAAAAGATGAGCGATCGTTTGAACAAGATGGATGTTCGTCTAGATAAACTAGAGTCTAAGTAGATATTATTCAAAATAAAGAATACCAGCAATTAGTCTTTAAAAGATAGTTGTTGGTATTTTTATTCATTTAATACTTTCTAAAAACTTTTTGCAATTTTGTTGGCACTTCTCAATAAGCCGGTCAATTTATTTAGTGGAAGAGCAAAATGCAAATCTTTAATTTTTGAAATTGATCAGCAAAGCGTCTGATCCCTGTTGGCAACTTAGTTGAAAGTTTTATTTACTTTCCAAACCTTACAAAGAAAGGAGGATTTAAACATGAAAAAAGTTAAAGCATTAAGGAATCTTTTTCTAGAAAAAGCCGAAGAAAAGCTTAAATACCCCAAAATTGCTTCAATGGTAGCTTACTTTGATCCAAAGGAAGATGGTACTTGTGAATTCATTATGAAAATTAAAACTGTCAATGAAGTTACTGATAACGAAGTAAAAAGCGAGGAAAAGCCTTTAGGCACTTGAAAGAGTTAGTTGATTTTTTGGTCTAAAAAGACATAAATTTACCAACAGTAAGTGTTAAAACTTGCTGTTGGTATTTTTATTCTCCCCCACGCTTTCCCGTTTCTCCACCGGGTTCAACCTCGCGGGGACTGTTTTGGACCAGGTGTTGGATTATGTTCCCTGGATTGGGAATGGGTACAGGTATGGGAATGACCACCGGGGGAGCAACAGTAGTACGAGTGGGGTAACGACCCAGGGACAATCCCAAAATGCATCCAGTAACGAACCCGCACCGACGTTTTCGAATGTCGGCGTTGGTCTCAAAGCCAACGTCCAAGCGACGCTCGGGGGCAGTCAGACGACGACTACCAAGGGTTCGCCTTGAAGAATCCTCGACCCAGCCAACCTCCAGCTCTGAACCGGCGCGGGGTGAAGGAATGATAAGGCTTCAAACAAACAAAGTGACGAAAACCACACCACCTTTAAAAGCGCTACGGGGATGGGCAAGCAGGGACAATCAGGTACCTCCGCGGGGAATCCCGATGCGTTAAAGCAGGATAAAATCACCAAGGAAAACTCGGGCAACCTCAAAACGGAGGATGGTACTGCTACTAATTCGACGACGGCATTCGCGTCGAATTACACCAACCTCCCCCCCAACCTCACCCCCACCGCTGATTGACCGAACGCGCTGTCATTCACCAATAAGAACAACGCGCAGCGCGCCCAGCTGTTCCTGCGCGGCCTGTTGGGCAGCATCCCGGTGTTGGTCAATAAGAGTGGTGAAAATAACTAAAAATTCCAAGCCACCGACCAAAAATGGTCGTATGCCGACTTAAAAACAGACCAAACCAAACTCAACCTCCCCGCTTACGGCGAGGTGAATGGGCTAATATTTCTAAAAATTCAGTTTATAAGCAAAACTTATTAGTTAATCAACAGTTTGAAAAAGTTTTATTCTGAACCTATTAAATGTTAATAAAACTTATGTAAGTATCACGAACACTCTTTTTAAATTCTTAGAAAAATGCACTTTTTTCCACTTTTTATAGCGATTTCTTTTGCATTCTTTAAACATAAATAAAAAGTTTTTCTGTATAATCTTCAGGCTGTTGACAACTCTGTCGACGCCGAAAATGTTCTTTCAAAACTGGATGCAATCTGTCAATTTTTCTGAGAGTTTGATCCTGGCTCAGGATTAACGCTGGCGGCATGCCTAATACATGCAAGTCGATCGGAAGTAGTAATACTTTAGAGGCGAACGGGTGAGTAACACGTATCCAATCTACCTTATAATGGGGGATAACTAGTTGAAAGACTAGCTAATACCGCATAAGAACTTTGGTTCGCATGAATCAAAGTTGAAAGGACCTGCAAGGGTTCGTTATTTGATGAGGGTGCGCCATATCAGCTAGTTGGTGGGGTAACGGCCTACCAAGGCAATGACGTGTAGCTATGCTGAGAAGTAGAATAGCCACAATGGGACTGAGACACGGCCCATACTCCTACGGGAGGCAGCAGTAGGGAATTTTTCACAATGAGCGAAAGCTTGATGGAGCAATGCCGCGTGAACGATGAAGGTCTTTAAGATTGTAAAGTTCTTTTATTTGGGAAGAATGACTTTAGCAGGTAATGGCTAGAGTTTGACTGTACCATTTTGAATAAGTGACGACTAACTATGTGCCAGCAGTCGCGGTAATACATAGGTCGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCAAGCGCAGGCGGATTGAAAAGTCTGGTGTTAAAGGCAGCTGCTTAACAGTTGTATGCATTGGAAACTATTAATCTAGAGTGTGGTAGGGAGTTTTGGAATTTCATGTGGAGCGGTGAAATGCGTAGATATATGAAGGAACACCAGTGGCGAAGGCGAAAACTTAGGCCATTACTGACGCTTAGGCTTGAAAGTGTGGGGAGCAAATAGGATTAGATACCCTAGTAGTCCACACCGTAAACGATAGATACTAGCTGTCGGGGCGATCCCCTCGGTAGTGAAGTTAACACATTAAGTATCTCGCCTGGGTAGTACATTCGCAAGAATGAAACTCAAACGGAATTGACGGGGACCCGCACAAGTGGTGGAGCATGTTGCTTAATTCGACGGTACACGAAAAACCTTACCTAGACTTGACATCCTTGGCAAAGTTATGGAAACATAATGGAGGTTAACCGAGTGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCGTTAGTTACATTGTCTAGCGAGACTGCTAATGCAAATTGGAGGAAGGAAGGGATGACGTCATATCATCATGCCCCTTATGTCTAGGGCTGCAAACGTGCTACAATGGCCAATACAAACAGTCGCCAGCTTGTAAAAGTGAGCAAATCTGTAAAGTTGGTCTCAGTTCGGATTGAGGGCTGCAATTCGTCCTCATGAAGTCGGAATCACTAGTAATCGCGAATCAGCTATGTCGCGGTGAATACGTTCTCGGGTCTTGTACACACCGCCCGTCAAACTATGAAAGCTGGTAATATTTAAAAACGTGTTGCTAACCATTAGGAAGCGCATGTCAAGGATAGCACCGGTGATTGGAGTTAAGTCGTAACAAGGTACCCCTACGAGAACGTGGGGGTGGATCACCTCCTTTCTAATGGAGTTTTTTACTTTTTCTTTTCATCTTTAATAAAGATAAATACTAAACAAAACATCAAAATCCATTTATTTATCGGTGGTAAATTAAACCCAAATCCCTGTTTGGTCTCACAACTAACATATTTGGTCAGATTGTATCCAGTTCTGAAAGAACATTTCCGCTTCTTTCAAAACTGAAAACGACAATCTTTCTAGTTCCAAATAAATACCAAAGGATCAATACAATAAGTTACTAAGGGCTTATGGTGGATGCCTTGGCACTAATAGGCGATGAAGGACGTGTTAACCTGCGATAAGCTTCGGGTAGGTGGTAAGAACCTCAGATCCGGAGATTTCCGAATGGAGCAATCCGGTAGTTGGAAACAGCTATCATTAATTGATGAATAAATAGTCAATTAAAGCAATACGTGGTGAAGTGAAACATCTCAGTAGCCACAGGAAAAGAAAACGAATGTGATTCCGTGTGTAGTGGCGAGCGAAAGCGGAACAGGCCAAACTTATCATTAGATAGGGGTTGTAGGGCTTGCAATGTGGACTTGAAAACGATAGAAGAAGCTGTTGGAAAGCAGCGCGCAAAAGGGTGATAGCCCCGTATTTGAAATTGTTTTCATACCTAGCGAGATCCCTGAGTAGCTCGGAAAACGTTATTTTGAGTGAATCTGCCCAGACCATTGGGTAAGCCTAAATACTAATTAGTGACCGATAGCGAAACAGTACCGTGAGGGAAAGGTGAAAAGAACCCAGAGATGGGAGTGAAATAGATTCTGAAACCATATGCCTACAACGTGTCAGAGCACATTAATGTGTGATGGCGTGCGTTTTGAAGTATGAGCCGGCGAGTTATGATAGCAAGCGTTAGTTAACCAGGAGATGGGGAGCTGTAGCGAAAGCGAGTTTTAAAAGAGCGTTTGTTTGTTATTATAGACCCGAAACGGGTTGAGCTAGTCATGAGCAGGTTGAAGGTTGAGTAACATCAACTGGAGGACCGAACCGACTCTCGTTGAAACGATAGCGGATGACTTGTGATTAGGGGTGAAATTCCAATCGAAATCCGTGATAGCTGGTTCTCGTCGAAATAGCTTTAAGGCTAGCGTGAGATCACAAATAAGTGGAGGTAAAGCTACTGAATGTATGATGGCGCCACCTAGGCGTACTGAATACAATTAAACTCTGAATGCCATTTATTTTATTCTCGCAGTCAGACAGTGGGGGATAAGCTTCATTGTCAAGAGGGGAAGAGCCCAGATCATTAAATAAGGTCCCCAAAATATACTAAGTGGAAAAGGATGTGAAAGTGCTAAAACAGCAAGGATGTTGGCTTAGAAGCAGCCATCGTTTAAAGAGTGCGTAACAGCTCACTTGTCGAGTGTTTTTGCGCCGAAGATGTAACGGGGCTAAGTATATTACCGAATTTATGGATAAGATTTATATCTTGTGGTAGACGAGCGTTGTATTGGAGTTGAAGTCAAAGCGTGAGCATTGGTGGATCCAATACAAGTGAGAATGCCGGCGTGAGTAACGCTTGGGAGTGAGAATCTCCCAAACCGATTGACTAAGGTTTCCTGGACCAGGGTCGTCCTTCCAGGGTTAGTCTGGACCTAAGCTGAGGCTGAAAAGCGTAGGCGATGGACAACAGGTTAATATTCCTGTACTTACAGTTAGACTGATGGAGTGACAAAGAAGGTTTTCCACCCCCATAATTGGATTTGGGGATAAATCATAAGGTGGTACAATAGGCAAATCCGTTGTGCATAACATTGAGTGATGATGTCGAGTGAATGAGTGATCAAGTAGCGAAGGTGGTATTAATCATGCTTTCAAGAAAAGCTTCTAGGGTTAATCTAGCTGTAACCAGTACCGAGAACGAACACACGTAGTCAAGGAGAGGATCCTAAGGTTAGCGAGTGAACTATAGCCAAGGAACTCTGCAAATTAACCCCGTAAGTTAGCGAGAAGGGGTGCTTATGTAAAAGTAAGCCGCAGTGAAGAACGAGGGGGGACTGTTTAACTAAAACACAACTCTATGCCAAACCGTAAGGTGATGTATATGGGGTGACACCTGCCCAGTGCTGGAAGGTTAAAGAAGGAGGTTAGCGCAAGCGAAGCTTTTAACTGAAGCCCCAGTGAACGGCGGCCGTAACTATAACGGTCCTAAGGTAGCGAAATTCCTAGTCGGGTAAATTCCGTCCCGCTTGAATGGTGTAACCATCTCTTGACTGTCTCGGCTATAGACTCGGTGAAATCCAGGTACGGGTGAAGACACCCGTTAGGCGCAACGGGACGGGAAGACCCCGTGAAGCTTTACTGTAGCTTAATATTGATCAGGACATTATCATGTAGAGAATAGGTAGGAGCAATCGATGCAAGTTCGCTAGGACTTGTTGATGCGAAAGGTGGAATACTACCCTTGGTTGTGTGCTGTTCTAATTGGTAACTGTTATCCAGTTTCAAGACAGTGTTAGGTGGGCAGTTTGACTGGGGCGGTCGCCTCCTAAAAGGTAACGGAGGCGTACAAAGGTACCTTCAGTACGGTTGGAAATCGTATGTAGAGTGTAATGGTGTAAGGGTGCTTGACTGTGAGACATACAGGTCGAACAGGTGAGAAATCAGGTCATAGTGATCCGGTGGTCCAGTATGGAATGGCCATCGCTCAACGGATAAAAGCTACTCCGGGGATAACAGGCTGATACTGCCCAAGAGTTCATATCGACGGCAGTGTTTGGCACCTCGATGTCGACTCATCTCATCCTCGAGCTGAAGCAGGTTCGAAGGGTTCGGCTGTTCGCCGATTAAAGAGATACGTGAGTTGGGTTCAAACCGTCGTGAGACAGGTTGGTCCCTATCTATTGTGCCCGTAGGAAGATTGAAGAGTGTTGCTTCTAGTACGAGAGGACCGAAGCGAGGACACCTCTTATGCTCCAGTTGTAGCGCCAGCTGCACCGCTGGGTAGTAACGTGTCTATTAGATAAACGCTGAAAGCATCTAAGTGTGAAACTATCTCAAAGATTAATCTTCCCATTTCGCAAGAAAGTAAGAGCCGTCAAAGACGATGACGTTGATAGGTTACAGGTGTAAGCATAGTGATATGTTGAGCTGAGTAATACTAATTGCTCGAGGACTTATTGGAAGCCTTTGGTAGGAAATACGCAGGATTGTCGTTTTTGTTTGGTGCCCATGTCGCTGTGGAAACACCTGGTTCCATTTCGAACCCAGCAGTTAAGCACAGTGGAGCCGAATGTAGCTGTTTCAGTGAGAATAGGAAAGCACCAAGCGTCAAGAAGATAAAAGAGACTTAGGCCTATGCCTAGGTCTTTTTTATTGTTTTGGTAAGCCAAAATAGTCCATTTTATAGCTGTTTTTACTTTGCGAAAAAAACAGAAAACTCCAAGCAATCAATTAAAGCTTTGAGATAATTAAAGGCTTTGGTACTAGTCAAAACCAAAGCCGATTTTTTCATGAACCAACAATTAAATACCACCAGGAAAAGTACAGCTGCTAGGGGCAGAATGGGACTTGTTGGTGGTATTTTACTCGTCATTGGTACCTGCATAGGGGCAGGTATTTTCTTTAAGAGTGAACGGGTGTTGCAAAACATGGGCGGAAACACCACTTTAGCCTTACTGGTGTGGTTAATGGCAGGGATTACCGTGATCTTAATGGGGTTGGCGCTGGTTGAAATTACTGCTAAAGCAGCCTTTGATGACTTGGCATTATTGTCATGAACACAAAAGTTTACCAATAACACCTTTTACAAGGCCTGTAAGCGCTTTTTAATCTGGATTTACTTGCCCACTACCTTCTTCTTTATGCCGTTGTATTTGGTGCAATCACTCCAAGATGGTTTGCGTGGTTTTGGGGTCGCCAACCACTTCAATACACCACATGATTGGGCCATTTGAATGGTCATAGTGCTGTTGATTAACCTGTGATTTTTCTTTACGAGTGGTCTTAGTGTTAAGTGAACCAGTGTGCAGAATGTCGTGCTATTATTGCTCAAAGTAATTCCCCTAATAGCAGTGGTTATTTTAGCGCTGTGATTGGGTGCAAGTGCTGAACAAATGGAACGCCAACCAGTGGTTCCAGTCAAAGATTTTACTGCCATTTCACCCTTCTTTGGTTGGTTTAGTGCTATGGGCGCGATTTTCTTTGCTTTTGATGGTTTTTACGTGTCAGCAGCAGCGAAAACGCAGTTGAAAAAACCAAAAAAATTACCGCAAGTAATGTTGTTGGGCTTAGGGATTGTGGTACTGATTTACAGCTTAATTGCACTAAGTGTGAGTTTAACCACACCTAACGGTGCCTTTTCGGGACTTGGTGATTGGTTAAAGCACAAAAAGCTTGGTTGATTCTTTGGCGTGCTCAATCTCTTAATAGCGCTTGGAGTAGCTGGGATTATTAACGGTTTTGTAATGTGAACGGGAAAGTTAACCCAAAGCTTAATTAAATCAGGAGAGTTGTGGGTACCTGATAAGTGTAAGCTCTGTTTAAATAAGCCCAAGCCAGTAGTGGGTTTAATCCATGCAGGGATTTTGATGGTGTTAACTACCGTAGCACTTTCTAGTTTAGGTGGTTTGTTGTACTTACCTAAAGTTAATGCTTCCTATGACGGCAAGGGCTTTAAATCAATGGGGTGCTTACTGGAGTTTGCTGACTTAATTGCCACTTGGACTAGTGTCGGGATCTTTTGGTTCTTGGGCTTAGTTTTACTGGGTGGTTTATTGCAAATCAAGAAACCGAAGCGGTGGTATTTTAGAACAACGGGTTGGCTAGCTGTAGTAGTGATTGGTCTCACTACTTTAGTGGTCATGGTGCAACCGTTTGTAGATTTAGGAATAGCAGTGTTTAACCGTAGTTATGAACGGATTGTGGCTAACACTATTTTGATTGCTATTCTTGTGATAATTGTTTTAGTAATGTTCTTTCCAACCGAACCAATTAAGCTAAGACTTTGACGCAAACGAATCCAAGCCATGGAAGCGTGCGGTGAGGATTGTGACGCTTGCGTGGAGTATTAACTAAAACTATAAAAAATAAAACTGGATAACCAAGCATGTAATATGAACAAGTTAAAGCGGCATAGTTAATTTGCGCTTAATTTCTTTTGGGGAGTTACACCGGGTAGAGGGCGAGTTTTGCTGGTATGCTTAGCGCGTTTTTGTAAGATTTGGCTTTCTTTCTAGTGTTGCTGAAATGCAATTTAAGTATGGTGCGCTAATTTTTAGCGGCTTTTTAGGACTTTCAATAGTTCTAGCTAGTTGTGGGGCGAGGGGTAAATTTGATCAAGTTGATGATGGTAAGATTAAGCTAGCTTCATCTTTAACTTCTAGAAGTGCGTCAGCGGCTTTACAAAAAGTTGTTGAAAAGTACAACAAGGTTAAAGGTGTAAATGATTACCCCATTGAGATTACCCAGATTGCTGGTGGTTATGATGGCGGGCGAACTGATCTCCAAACCCGGGTTAGTGTAAAGGATAAAACCAGCTTTTACAACATGATTCTAAACTACCCTGATTTAGTGTCGGTGTTAGCCCGTAACGGGATGGAATTACCGTTTGATGGAGTCAATGTTGACAAGTTATCTCCCAACTTCTTAAAGTTCAATGAACGCATTAGTGGCGTAGCGAAAAAAGCTAACTATGCCATTCCCATTTCGATGTCAACCGATATCTTAATCCTGAATGCACCGGTGTTGCACTATATTTTGAACAGTGCTAAGAAGAATGATGGCAACACTAAAGTTCAAGTTAAAGCTCAGTCAAAAGATTCACAAACAAAAGTTAAAGGTACTATGGAAATTGGCACTGATGAAAGTACTAAAAACTTATGAAGTGATATTCAAAAGAAAGCTGGCGAAAATGGCAAAGCTACTACTGAAGGCACGAAGAAGGCAGCAGCAAAGTCTACTCATTTAACTCTGTTAACTAAGTCGGAACAAAGTACTCAAGGTAATAATGGAGCATCTGAAAGTGATAAAAAAATTGAAGAAACTTGGGGTACTTACAGTGAAGTTGATGGTGGTCTAAAGAATTACACCTTTAAAGCCGATGTGTTTGATACTTGACATGGGTTGATCGATTTTTCAACGCGCGTAGCAAAATCATTTAAAAACAAAGTTAGTGATATTTCCACTAAAAAGGGCACTGACATTCAGGGCGTTTTAGGTCTAGACAGTACACCAAATGCTTTGTTTACCTCAGTTTTTGCTGCAGGTGATAGTAACTTTGATAACTTCTTTTATAAGGTTAAAGATGGTCGCGCTGACTTTAGTAACTTTAACGAAAACGGTACCTCTTACAAAAATCTGGAAAAGGTTTTTAATGACTATAAAAAACTCACAGACAGCAACGGTTTGTTTGTCAATAAAGGGGGTTCTTACACCTCTAACTTCCAGAAGTTTCACCAGTTAGCCTACAGCATTTCCTCCTCTTCTGGTTATGCTTATGCGTTTGCTGGTGAAAATTCAAAACGTTTAAAGTTCAACGACGATACTTTCATTGAGTACCCTAGTTTTACCCAGGAAATTCATGCGCCTGGTCAAAGTAGTCAAAAAGAAGGTGGACAGCAACAAAGCAACAGTAAAGATAACGGCAATCTTTTAGGCACTTTTACTATTGAAGCAGCGAAAAGTAAAACAAAAACAGAAGTTAAAAAAACTGAAGACACACAAAATCAAGGCAAGAAGGCTGAAGGCACACCTAACCAAGGTAAAAAAGCCGAAGGGACCGAAAACCAAGGTAAGACTATTTTCCTTTATAAAACATCAATTCCTAATGATAAGCAAGATGGTGTTGACGCTGTATTAATTAAAGACAAGTAGTTAATTCAAAAACTAGAGCAAGCTAAAAATAATAACGGAGCCGAGAAAAAGGATAAGTCTGAAACTAAAACCAATGAAAACAAAGGCACAGTCATCGGTTACACCACTACGAGTTTCCTGAATGCTGACAAAAAGCATGTCTTTGAAATTAGCAAGCTTAATGATGAAAAATACGACCGCAAGATTATCGTTGGTGCTACTGAGGAACAATTAGATCAATCAAGTACGTTACAAAGTGCGGAAGCAATTATCCTGCCAGCACCTGGTAAATACAGTGCCGAAAACCAGCATAAGGTAATGATGACTCAAGGTCCTAATCTAATTGGTATCCATACTAATACCAAGGAAAATGAAGAAACGCAAAAGTTTGTTAACTGGTTCTTAAATACCAGTCTAACTTGAGATAACAACGAAAGCAAAACACCAGCGCAGTACTTTACCGAGTCTGCTTCTTACATCCTGCCTTTAAAAGAAACTTTTACCGGCTCTAACAACAAAGGTCAAAGCGGCAAGAATGACGGTAAGAATTCCAATAACACCTTTAAAGCCAAAGCACTAGAGCTCTTTAAGGAACAGAGCGAAAACAAAATAGTGGGTTACAGTGATCCAAGCGATTTCAGGGGTGGTAAATTCCGCGAATCAATTGGTTCTGCCTTTAACGCCACGGTCAATTCACACGTTGACTTTAATACTTTCGTTGCTAACTTCCGTGCTAACTTAGGTTCTGGTTACGATAAATAGTTAATCCCACTTCTCCCCCACGCTTTCCCGTTTCTCCACCGGGTTCAACCTCGCGGGGGCACTCCTCGACCAGGTGTTGGATTATATTCCCTGGATTGGGAATGGGCACAGGTATGGGAATGATCACCGGGGTGTGGATGATATAACCGCGCCTAAAACCGGCGCGGGGTCGTCCAGCGGAACTAGTACGAACACAAGTGGTTCGCGTTCCTTTCTCCCGACGTTTTCGAATGTCGGCGTCGGTCTCAAAGCGAATGTCCAAGCCACCCTCGGGGGCAGTCAGACGACGACTACGGGGAAAGATATTCCCAAATGACCCACCCTCGACCAAGCCAACCTCCAGCTCTGAACCGGGGCGGGGTGAAGGAATGATAAGGCTACTGGGAATAACACCTAAGGCAACGAAAACCACACCAAGTTCGCGAGTGCTACGGGCACGGGCCAGCAGCAGGGTGGTTCTTCAGGTACAACTAATTCCGCGGGGAATCCCGACTCGTTAAAGCAGGATAAGATTAGTAAGAGTGGGGATAGTTTAACCACGCAGGACGGCAATTCGACCGGTCAACAAGAGGCCACCAACTACACCAACCTCCCCCCCAACCTCACCCCCACCGCTGATTGACCGAACGCGCTGTCATTCACCAACAAGAACAACGCGCAGCGCGCCCAGCTGTTCCTGCGCGGCTTGTTGGGCAGCATCCCGGTGTTGGTGAATCGAAGTGGGTCCGATTCCAACAAATTCCAAGCCACCGACCAAAAATGGTCCTACACCGACTTACAGTCGGACCAAACCAAACTGAACCTCTCCGCTTACGGTGAGGTGAATGGGTTGTTGAATCCGGCTTTGGTAGAAACCTATTTTGGGACGACGCGTACGTCTAGCACTGCGAACCAAAACAGTACAACCGTCCCCGGTATCGGTTTTAAAATTCCCGAACAAAATAATGATTCCAAAGCCACCCTGATCACCCCCGGGTTGGCTTGAACGCCCCAGGACGTCGGTAACCTCGTTGTCAGTGGCACCACGGTGAGCTTCCAGCTCGGCGGGTGGCTGGTCACCTTCACGGACTTTGTCAAACCCCGCGCGGGTTACCTCGGGCTCCAGTTAAGTGGCCTGAATGCCAGTGACAGCGACCAAAGGGAGTTAATTTGGGCCCCCCGGCCCTGAGCGGCCTTTCGTGGCAGTTGGGTCAACCGGTTGGGCCGCGTGGAGAGTGTGTGGGATTTGAAGGGGGTGTGGGCGGATCAAGCTCAGTTGGCCGCGCAAGCAGCTACAAGTAGTACCACCACCACCGCAACAGGGGCTACCTTACCGGAGCACCCGAATGCTTTGGCGTACCAAATTAGCTATACCGACAAGGATTCGTACAAGGCTTCCACTCAAGGTTCGGGTCAAACCAATTCCCAAAACAATTCGCTCTACCTCCATTTGATTAAACCTAAGAAAGTCGAAAGCACGACCCAACTCGACCAGGGCTTAAAAAACCTGTTGGACCCCAACCAGGTTCGCACCAAGCTGCGCCAAAGCTTTGGTACAGACCATTCCACCCAGCCCCAGCCCCAATCGCTCAAAACAACGACACCGGTGTTTGGAGCCATGAGTGGTAACCTCGGCAGTGTGCTTAGTGGTGGGGGTGCTGGAGGAGCAGGCAGCACCAATTCGGTGGACCTCTCCCCCGTTGAACGGGTGAGTGGGTCACTAACCATTAATAGGAATTTTTCTTATTAATAGGAACTTTTCTTTCAAAAAAGTTTTGAAGTTATTTTGGTGTCTTTACTAACTTAAAGCAATTTAGTGAACGAATACCCAAAAAGTATGAAATTAGTTAAATTAACGAGGCTTCACCGCTTTTTAAAACAAGTTCAATTGCAACTTGTTCCTTTGTTTAGCCAAAACTTTTTGCAAGTTTTATTGGCGCTTCTCAATAAACCGGTCAATTTATTTAGTGAAAACACAACAATGAAAACAAATAAAAAATACGCCTTTGCAGCGTTGCTAATGCAAGCTCAAGCTAAGCTCCCAAATTCATTTAAATTCTTTCTTTCAAAGAAAGGAGGTATGGCTTAATGAAAGAGAAAATTCCGTTTTATAACGAAAAAGAATTCCATGACATGGTAAAAAAGACCAAAAAGGGCACTTTTTCGGGATGATACATCATTGACAAAGATAACAAAAGTGTGGAATTCTCTGGCAGTTTCAACAGACAATTTAAACTTAACAAACCGGTTATACCAGTGAACACGGAATACGTAACGCGTAAAGAATTTAACGAATATAAAGTTTCAAACGACCAACGGCTTACAAAGATTGAAACCACTTTGGCCGCCCAAGGTGAACAAATCAACAAATTGACTCAAACTGTTGAAAAACAAGGCGAGCAAATCAATCAATTAGTTCAAGTTGTGCTTCTTCACGGTGAACAAATCAACAAATTGACTCAAACTGTTGAAAAACAGGGCGAGCAAATTAAAGAACTTCAAGTGGAGCAAAAAGCTCAGGGTGAGCAAATTAAAGCTCAAGGAAAACAAATAAAAGCTCAAGGAAAAACGCTTAAGTCGATCCTACAAGCACTTGGAGGAATAAATAAACGCTTGGACAAAATTGATCCACCTAAATAGTTTTTAGGCTAATAACAAAAGTGCCAGCAATTAATTTTATAAAGCTTATTTGCTGGTATTTTTATTCACAACCAATAAACAAATAAAAAGCCATCCACCGCGAGGTGAACGGTAGACGGCTAAAAATTTTTAATGATTTTGAAAACTAACGAAAAGTGTTAGTTTGAGTTGTAAAGCAGTTATGAATGTTTGTTCCTTTAAAAATTTGTTTGTTGTTTAGCTAAATATGTGCTGAAAGCCATTGCGTTAACATAAATAAATTAAGACATAAAAAATAAACACATTAATCAAATAAGTGTGGGAACAGAAAATCAGGCTTTCAACACAAAATTTTCGTTTTTCTGCTCCCACTAAATAAATTGAGTTGGTTTTTAAAAACCGCCAAAAAATTTTTAATTTAGTTTTTGCTACTTTCCCCTCCCCCTCACGGGTGAAAACCCCGGCGCTTGGGCCTTAGTGCGCGACAACAGCGCCAAGGGCATCACTGCCGGTAGTGAAAGTCAACAAACCACGTATGATCCCACTCGAACCGAAGCGGCTTTGACCGCATCAACCACCTTTGCGTTACGCCGGTATGACCTCGCCGGGCGCGCCTTATACGACCTCGACTTTAGTAGGTTAAACCCACAGACACCAACGCGCGACCAAACCGGGCAGATCACCTTTAACCCCTTTGGCGGCTTTGGTTTGAGTGGGGCTGCACCCCAACAGTGAAACGAGGTCAAAAACAAGGTCCCCGTCGAGGTGGCCCAAGACCCCTCCAATCCTTATCGGTTTGCCGTTTTACTCGTGCCGCGTAGCGTGGTGTACTATGAGCAGTTGCAGCGGGGGTTAGCGCTCCCTAACCAAGGGAGTTCGTCAGGCTCCGGTCAACAAAACACCACCATTGGCGCGTATGGGCTGAAGGTGAAGAACGCCGAGGCGGACACCGCGAAGAGCAATGAAAAACTCCAGGGCGATGAATCCAAGTCTTCCAATGGATCTTCAAGCACTTCCACCACCACCCAACGTGGGGGTTCGTCAGGGGACACCAAAGTCAAGGCGTTGCAGGTGGCGGTGAAAAAGAAATCCGGGAGTCAGGGCAACTCCGGTGAACAAGGCACCGAACAGGTGGAACTTGAATCTAATGATCTCGCCAACGCTCCCATTAAGCGGGGCGAGGAGTCGGGTCAGTCCGTCCAACTAAAGGCAGCCGACTTCGGCACCACCCCATCCAGTTCGGGATCAGGCGGCAACTCCAACCCCGGTTCCCCCACCCCCTGAAGGCCGTGGCTTGCGACCGAGCAAATTCACAAGGACCTCCCCAAATGATCCGCCTCGATCCTGATTCTGTACGATGCGCCTTATGCGCGCAATCGTACCGCCATTGATCGCGTTGATCACTTGGATCCCAAGGTGATGACCGCGAACTATCCGCCCAGTTGAAGAACGCCCAAGTGAAACCACCACGGGTTGTGGGACTGAAAGGCCAGAGATGTTTTGCTCCAAACCACCGGGTTCTTCAACTCGCGGCGCCACCCCGAGTGGTTTGACCAGGGCCAAGCGGTCGCGGATAATACCCAAACCGGGTTTGATACAGATGACACCGATAATAAAAAAACAAGGCTTTCAAAAGGAAGCTGACTCCGACAAGCCGGCCCCGATCGCCCTCCCGTTTGAAGCGTACTTCGCCAACATTGGCAACCTCACCTGGTTCGGGCAAGCGCTTTTGGTGTTTGGGATTTGTTTGTCTTAATTAACTAACAAATTAACTAACAAATTAATAAATAGGTCTATGGATTAATTACCTCACCCCCTACACGCCCTTTACGACACCGCTCAATGGGGGGCTGGATGTCGTGCGGGCCGCCCATTTACACCCCTCATACGAACTCGTGGACTGAAAGCGGGTGGGACAGAACAAGTTGGTGGCGCTGGTCCGCTCAGCGTTGGTCAGGGTGAGGCTTAATGATGGGACGAGTTCGGATTCCAATACCACCACTGATGGGGCGCTTACGTTTGACACGAATGAAAGTCAAAAAGCTTTAGGCTCCACGGACACGCAGGGTTCCGGGTCTAACTCCCAAGACTTCGCCAGCTACATCCTCATCTTTAAAGCCGCGCCCAGGGCCACGTGGGTGTTTGAACGGAAGATTAAGTTGGCGTTGCCCTACGTTAAAAACGAATCGGGAGCAGGGGACTCCACCACAACTAACAGTGGCTCCCTCTACAAAACCCTCCAAGACCTCCTCGTCGAACAACCCGTGACCCCTTACACGCCCAATGCGGGGTTAGCCCGGGTGAATGGGGTTGCTCAGGACAAGGTGCATGGGTTGGAGTCTGGGTGAGACAACCAACGTTCCCAAAAAAACCTCACCAACAACCCCGGACCCAAAGCCGTCACCGGCTTTAAGCTCGATAAGGGCCGCGCGTACCGGAAGCTAAATGAAGCTTGACCGGTGTATGAACCCCTGGATTCGACCAAGGAGGGGAAGGGGAAGGATGAGAGCTCTTGGAAAAATTAGGAAAAAACAACGGCGGAAAATGATGCCCCGTTGGTGGGATCAACCGGGAGTCAAATGGCTTCTAGTACCAGTTTGGGCAATGTTGGGGACACCACTGCCCTAACTCCACTTTTAAGAGGTGCTACTACTACTACTACTACCGTCCAGCTAAAACAAACAGATTCTAACAGTCAAGATCAACAAAAATTCCAAAAATATTTAAACACCGCCCAGGCCCTGCACCAGATGGGGGTGATAGTGCCAGAACTTAGCCAAGGCGGTTGGCAAAGCCAAACAGCGCGGCACAGTTCCACCCGGGGACTACAGTCAGCCGGGGCCAATCGTGGCGCTAGTTTAGCGGCACGCACCACCACAACTGTGGGGGTGGGTAGAAGAGATTCCACTTCCACCACCTTGGAACTACCAAACGTCATCACCCAGCTTTATCATACCTCCACGTCCCAACTCGCTTACTTAAATGGCCAGATCGTTGTGATGGGTTCAAACGCGGTACCGAGTTTGTGGTATTGGGTCGTGGATGAGCGAACCACGTCCGGGAGGGCGACTTGATGAGCGAAGACACATTTGAACTTCGGCACTGAAGTTCAAAAAAACTTCGTCGAAAACCAGTTGGGGTTTAAATCCGAAGACAACTCCAACACCTCCTTGACGAACTTCAAGAGTCAAGGCTTAACTCAGCCAGCTTACTTAATCAGTGGCCTTGACGTTGTGGCCGACCACCTCGTCTTTGCGGCCTTTAAAGCGGGCGCGGTGGGGTATGATATGACGACTGATTCGAACGCTTCGACCTACAACCAAGCGCTCACCTGGTCGACCACGGCCGGGTTGGACAGTGATGGGGGGACAACAACCTGGTGACGAATACTGCGGGGTTAAACGGTCCGATTAACGGCTTGTTTACCCTGTTAGACACCTTTGCCTATGTGACCCCGGTGAGTGGGATGAAAGGGGGGAGTAAGAATAACGAAGAAGTGCAAACGACCTACCCGGTCAAGTCCGACCAAAAGGCCACCGCCAAAATTGCGTCCTTAATTAATGCTTCGCCCTTAAACAGTTATGGGGATGATGGCGATAAAAAAGCCATCCACCTAAGGTGAAAGGCAGACAGCTCCAAAATATTTTTGACTATTGGAAAACTAACAAAAGCTGTTAGTTTGATGTAGAGGCAAAACTATGAATTTACTAAATTTATTTAAATCAGCGGGAGCAGGCTCGTTTTTTCAACACAAGTCACTCTACCTGCTCCCACTAAATAAATTGAGACAGATTTTCAAAGTAGCCAAAGAGTTTGAATATTTTTGCAATTTGGGTTTACAACTCCATCTAACTATTAGTAGGGTTTTCACTTTTGAAATTTTTTTTGGATTAATTTTGGCACCCCGCCTTTTTTAAAGGCATTTAAGAGATATAAAAGAACAACTCATGTTTCTAGATACTTTCAAAAGATTTTTCGGGTAACTTAGATCACCTTTAATGTTGTTGTGGTTGGAGTTGATTTGGTTTAAATGGCAAAGGTTATGGTAAAGGTTTTTGGCACCTTTCTTTTACTTGTGCAATTCATTTATTAGAAGGGCAACAAATGGTCTTTTTGAACTAATCAGTTGTTGCCTAGCTTTATTTAAAAAATGATGAGTAAAAATAAATTCTTTACCATTACTAAAGAATAGTACAGGAAGTTTACACGCAATAAAATTAAACTTCTTTTTAAGGTCCAATGCACCGAAAAGAAAAAGGGTAAACGTAGGTTCAAAATATTTCAGCAAGTCGAGGATAAACCAAAACCACCAGTTAAAATAATTGAAAAGGAATCTAAGGGACCGGAAGAACCAAAGGATCCTGGTATTAAAAATGGACCTAAGGGTCCCGGTGGACCAAAAGGACCTAAAAAGCCACCTGTTCCCGATGATCCTGATACACCAGATGTTCCTAAAGGCCCCAAAGAACCAGGCGGTTCAAAAGGTCCAGGTGGTCCCGATGATCCTAATGGAGGAAAGAAGAAAGTGCCTAAACCAGATAAGTATGTAACACATCGCGAGCTTGATGAGAAACTTACTGAGTTTCATGACAAGCTTATGGTAGAACTTGATGGTAAGTTTGCTACTAAAGCTGATTTAAAACGGGTTGAAGATAAAGTTGATGTTTTGTTTGAATTGCAAAAAACGCAAGGTGAACAAATCAAGGTTCAAGGAAAACAAATCGAACAGTTAACTGAAACTGTTCAAAAACAGGGCGAACAAATAAAGGAACTTCAAGTTCAGGTAAAAGCCCAAGGTGAAGAAATAAAAGAAATTAAAGTTGAACAAAAAGCTCAAGGCCAAACCTTGCAGTTAATTCTGAAAGCACTTGAAGGAATAAACAAGCGACTAGATAATTTGGAATCGAAATAATCCCATTCCAAAATAAAAATACCAACAGTCAATCCTTAAAAAGTTCACTGTTGGTATTTTTATTCACTCAAAAAATTCTTAAAATTTTTTTGTAATTTGTCGTCACTTCGAAATAACTGAATCAATTTATTTAGTGGAGGAGTAATTATGAGTAACTTTGAATTTTGATTAATCCTTCAGAAAGGAGGATTTGATTATGAAAGATGAAACAAATATTTGTTCTCAATCTTTAAAAAGACCTTTAAGCGAAGACCAATTGATAGAGTATTTGAGTACTTATGAAATAACTTTATTTGAGGTTTATGCTGAGGATATGCTAGATGGCAATTGCAAAATTTATATACATGCTAAAGGACGTCCAAAAGACCCAGATTGAAAAAGAAAGACTGATAAAAAATCTTTAGAAATTGAACGATATGTAAAGAAATTTATTAAGTTTTTAAAGAGAGAACAGAAAAAATAAAAACAAAATACCAACAGTTAATTCTTAAAAAACGCGCTGTTGGTATTTTTATTCAACGCAAATAAAGGAATAAAAAAGCCATCTACCCAGAGGTGAAAGGCAGACAGCTCCAAAATATTTTTGACGATTGGAAAACTAACAAAAGCTGTTAGTTTGATGTAGAGGCAAAACTATGAATTTACTAAATTTATTTAAATCAGCGGGAGCAGGCTCGTTTTTTCAACACAAGTCACTCTGCCTGCTCCCACTAAATAAATTGACAGGGTTTTATAAAAAAGCCAAAAGCTTTTAAAGTAACTATTTATTTGCAAATTAGTGCAAATATTCGTGTGCTATTTAAATTAACTATAGCACAATTAATAACAGTTGCATGATTGATCAAAGTAAGTTAATTGAAAGGTGAAAAACTACCTTTGAAACAGCACAAAATCCTACTGAATTATTAGCTTTTAAAAACAGCTTCCGCAATGCTGATTTAAAGCCTTTACTTAGTCAAATTAAGGAAACCACGGACATCGAAACTAAGCGCCATTTAGGCCAACTTTACAAGCAATTGGAAAGCACCTTGCAAACACTCCATGATACACAGCTACAAGTCTTTACCCAAGCCCAGTCTAGCAGTGTATTAACTCATGGTGATGTGATGTTGCTAGCAACCTCTTTTGCCCCTGGTTCTAGCAACATTATTTACCAGGTAATTGATGAGTTAGTGAACTATTTTAAGAAGTTCTTGTTTACGGTTAACTATGACAGTGAATTGACTACTATTGCAGACTGTTTTGACCTGCTAAACATTCCCAAAGACCATCCTAGTCGTAACCTTACTGACACCTTTTACTTAGACAAAAACCGCTTACTGAGAACACATTGTACTGCTGCTACTTTACGTGCTGTTAAGGAAACGAAAAAGAGTAATAACCCCGATATTCGGATTGCTTCCTTTGGTGCTGTTTTTCGTAAAGATGATGACGATGCCACCCACTCACACCAGTTCAACCAACTGGACTTCATGTGGATTAAAAAGGATTTTTCTTTAACTAACCTCAAGTGGTTTATGCAGAATATGATTAACCACATCTTTGGCGAAAATACTTCAGCGCGTTTTCGTCTGTCCCACTTCCCTTTTACCGAACCTTCCTTTGAAATTGACATCCGTTGCTGGTTGTGTCAGAATGGTTGTGGTGTATGTAAGAAAACACGTTGGATTGAGGTATTAGGGGCGGGTATCCTCCACCCTCAAGTGATGGCTAACATGGGCTTTAGTGACACGGATAATATAAGGGGTATAGCAGCTGGCATTGGGATTGAGCGGTTGGTAATGTTGAAACACGGCATTAGTGACATCCGTGACCTTTACGACAATAACTTCAAATTTTTAGCCCAGTTTACTGATTAGCGATGTTAATTTCTAAAAAAACTTTAGCTGTTTTAATTCCCGAAATTACCCACGTTTCCAACAATGAAATTTGTGAAAAGTTACAGCAAATCGGGATTGAAGTGGAAGCAATTCGCGCCTTTAAAAACCCTGATTACTTACAGTTGGGTATTCTCCGCGCTGTTACCCCCCACCCCCATGACAACCACTTGTATGTGTGTCAAGTTCAAATAGACAAAAACAAACAGCTCAATGTGGTGACTGGGGCAGTTAACATAGTTGATCCCAATAATCTCAATAAACACGTGATTGTCGCCAAAAAAGGGGCAGAACTGCTCAATGGTTTAATCATTAAAACGAAGAACATTAAGGGAATTATCTCGGACGGGATGTTGTGTAGTTATGTTGATATTAATCCGTTTAGTAAGCATTTAATTGCTGACGGTGATGACACGCATGCGATTGTGCTAGACAACATTAACCGCGATGAATTTGGTGATTATTTAAGTTTTTTAAACCTCGATGACGTGGTGTTTGAGGTTACTTTACCTACCAACCGTTCTGATTTACAGAGCTTAATCTTCCTCGCTAAGGAACTAGCAGCAGTGTTGAAACGTCCGGTCTTCTTGGAACAGAAAACCACCATGACCCTGCGCGAGTTCTACCGCTTTCCGCTTAACTTAAGAAACCGTGCTCAGGCCAATTTCTTTGGTGGACTTTTTTTGCGTGATGTAGCAATTACCAGTTCCCCTTGAACTACTAAGGGTTTGTTAATTAACCAGGAGTTACGTCCAGTCAACTGCTTTGTGGACCAGGCTAATATGGTAACTGTTTACACTGGTCAACCAATCCACTGCCATGATGCTGACAAGATCCATGGAAGTGTGGAACTTAAACTAGCCACCCAATTGGAAACGATGTTGGCCTTAGACAACAAGGAGTATGAGATTAAACCGGGTGATCTAGTGGTAGCTGATGAACAGGGCACAATTGCCATAGTCGGTATTATTGGTAGTAAGCGCACTATGGTCGACAATACGACCAACAACATCTTTTTTGAAGTTGTTAACTATAACCATGAGCGAATTAAGCAAACCGCTCAGCGTTTAGGAGTTGCTAACTTTGCTAGTAGGTTAATGAGTAAACCAATTAGTTTACAAGCTACCGAAAACTGCTTGAACTATTTGCAAAATAACTTCCTCAATCCCGAAAGCATTGGCAAGATTAGTAAGTTTTCCAGCACCATTAAAGCCCCAGCTTTTAACCGCAAAATTTACCTCAACTTTAATCAGTTACGGGAGTTAATTGGAGTGACTAAAAAGCAGTTAAATGACCACATGATCCGTAACTACCTTACTAGTTTAGGGTTCAAAATGGAAAACCAAATTGCCCGCGCCCCCGCTTACCGGCAAGACATTACGGTTTGACAAGACATTAGTGAAGAGTTGTTAAAGATCTTGGACCTCAACAAGATTAAAGAGGATGAAATTTTAAGCTCCACTAAACTAGAGAAACACGAAAAACTCAATGCTTACGATGCGTTGCAAAAGCTACGCACTAAACTGCAAACCTTGGGCTTTCACAATGTGATTACTTACCAGTTAATTTCACCAGAACGAGCGCGTAACTTTAACCTGTTTGGGTTGAGTAATTTGTGGGAAATTAAAAACCCGCTTTCCAACGAACGGAGTGTTTTACGGGTTGGTTTAATTGACTCCTTGTTGCGAGTAATTCAAAAGAATGCCGCATACAAAAATAAGTTAGGCAACATCTTTGAGTTTTCTTTTGTCAAAACTAAGGACAGTAACCAACTCCACATTGCTGCTCTTTGATTGGAAAAAATGTTTGGTTCTACCTACCAAAAGGATCAAGGCGTTAGTGTCGATATTCCCGCCATGAAGGGCTTAGCGCAGTTAATTATTTCCAACTTTGGTTTTAACTGCGACTTTGAACCAATTACTGAAGGTGAGTACTTCACTAAGAACGTCGGTTTAAAACTAGTGGTGTTTAACGAACAGATTGGTTATGTTGGTTTAATTAAGGATGAGCTGTTGGCGCCTTATGATTTAAAGGGTAGACCGGTGTATGGTTTGGAAATTAACCTAGACCGCTTGTTGAACTCCTTAAACCGGTTGGAGCGCAGTTATACCCCGATTAGCAAACTACAAGATGTCTTTAAGGATATTACCTTTAGCTTCCCCCGTGATGAAAGTCACTTTGAAACGTTTGTGAAAGCGATTAAAAAACTACAAACGATTTTTAAATGGGAATTGATCTCGGTGTTTGACACCGAAAAGGATGGTGTACCAATCACCAAATATACAGTGCGCTATTACTTAAAGAATTTCACTAATACACCATTAACGTTAGAACAAATTAAAGCAGTGGAAACGCAGTTAAAACAACAGTGTGAACTAGCTAAAATAGCACTCGATCTTTAGAAAACGTACTTTTTAAAAGATTTCGTTAGGTCTGTTCGGTTTTCGGTGTCTTCTTTTATTAAACTGATTCGGTTTCTATTTTTAAAGTAATAGAAATAAAGCTAGCCAAACAATGACAAACCAAAACCTACAAGACTTTCTGGACGGAGTTTTGTTAAAAGAGGAAAATTTAAACAAAATTACTGAAGTTTCCCTAGAAAAGGGATTACCGCACCTTTTAGATGAGGGTATCCATAACGAAGATAACTTTCAGATCTTTACGAGCCAAAAGATTGTGGCAGACATGATCAAAATGGTCGGTGTAAAGGAAATGAAAAATGTCCACACTACTGTTTTAGAACCAACTAGTGGTGATGGTGCTTTTACTTGTGCTATTTTGGATTTACGTTTAAAAAACATTAAAAAATCAGACAGCTTTTTAAAAGAAGCTTTAACCGGTCTATCCACCATTTATTCAGTTGAAATTGACGAAGAGTTGGTTTGGTTACAACGCAATAACCTTTACTCTATCTTTTTAGCCTTCTTAAAAAAGCACGGTATTTTTAACGAAGAGTTAAGTCTCTTAGTGAAAAAAATCTTGATGATTAATGTGAGTTGGGGCGAGTTAATCACAGAAACCAAAGATAACGAGAACTTCGTTTGCAACTACGATTATTTTTTAAGGAATAAAAAGACAAAGAAAAAACCCGCAAAGCATAAACATGAATTTCCAGAGGGTTTATTTTCCAATGGAGAAGGTAAGTGCAAAAGTTTGTTTGTTAATTGGTCATTTGCTGATGTCATTAATGAAATTGCTCATAATTCCAATTTTTACTTTGCCAAAATAGCAAAGATACCTGGTGAAATAATTCAATTTCAAATTAATTAGTATGAGTCAAAATGGCGATAAAGACCACGAATTGATTCAGGGAAAGGTCAAAAGCAAAACTGAAATAGAAGATTCCGTTATATTGGATCAAGACACTTTGCAAGGCAAGGGTGAAGAAAAAACTAAATTAGATGAGTTGATTAGGTGAGAAGCCGAGAAAAACGACTTGCTCAAGTTAATTGATAACGTCGGTAATGATGAAGTTTTTACTACTGTAGAAACTTGTCAAAGAATGCTTGACGATCTCTTTCCTCAAGACCATGAAGTTTGATCGAATCCGGACTTAAAGTGATTAAATCCTTGTGATAAAAATGGTGTTTTCTTTCGTGAAATTGCGTTAAGGCTAGACAAAGGCTTGGCCAAAGTTATACCGGATGAATATGAACGCAAGAAACACATCATGACCAAGATGCTTTTTAGTATTGACTTAACTAAATTTACTAGCTTGATGGTTCGTCGTACGCTTTACTATTGCATTAAAGCTAACAAGCGTAAAACTAGTGAAGATGAGGGCTGTGCAATTGCAAATGGCGCATGATTTAATAACGAATGCGGTAATGTTGTTAAACCTTACAAGGAACATTATTTCGACAAACAAGGAAAAAATAAAAAATGTAAGTTTTGTAGAACAGATGAGAAATTTAAATACCAACAATCTTCTTCAAATGAAAAATACGCCTATGACTTCATTCATTTAAATCCAAACGAATATGAAAGCTACTTTAAAACTAATTTTGGTGTTATGAAATTTGATGTAATTATTGGTAATCCACCTTATCAGTTAGCAAATAATCGTGGTGGTGATGCTGGTGATGGTAATGGAGCAAACCCCATTTTTCAAGAATTTATCTTAAGAGCACTTGAATTACAACCAAAATATTTAGCAATGATTGTTCCTGCAAGATGAATTAATTCCTCTGAAAAAGTTTTTCTAAAGTTCAGAGCAAAATTACAAAAATCAAATGGTTTTAAAGGAATTAATATTTTCTTTGACTCTAAACATTGCTTTCCAAACAGACAAATAAAAGGTGGTGTTTGTTACTTTAATTGACAGAATGGCTATGAGGGTCAAACACTCATAACCACTAAATCTTCAAAAAAAGGCAAACAAGAAGAAATTGAAACTTATAAGAGGGACTTTTTTGCACCTGTTTTAAACAATACTGAGCAAGTTATTTTCCGCAAAAAATTTGACACGACATTTATAAAAAGGTAGATTCTTTTCACGCTAATGAATTAATCCATGCTCTCAGAGAGAGAGAGAGAGAGAGCAACTTAGAAGATCAGCCTTCTAATGGAATAACACACACAGAGAGCAATTTAGAAACTAATTTCGAAAGTTTCTCTAAACGTGTATCTGGCAAGGATCTGTTTAATTTAGCAACTAATTTTTTTGATAAGTTTCCCTTAGTTGTAAAAGACAAAAAATTTCCTGGTGCAATTTCCATTTATAGCAGTTCGGGCGTAAAATACGCCGAACTGAAAGAGATCCCTAATATAAAGAATAAGGAACAAATTTCCAAATACAAGATCATTATGCCCAGAATAACTGGTAGCGGTGTGTTTGGTGATTACAGACCCAATATAAAAATTGTTGAACCTACTGGAGTTTGCACTCATAGTTATTGTAGTTGCTTATTTAACGACAAATAAGTTTGAAGTTGAAAATGTCTTTTTATACATGAAAACTAAATTTTTTAGATTTTTTGTCGAGATTTTTAAAGCAACCATCAATATCAGTTCACATAACTTCAAATACGTTCCCATTCAAGATTTTTCCCGTCCTTGAAATGACAGGGAGCTTTATCAAAAATACAACTTAACTCAAGAAGAATGACAATACATCGAGAACAATATTAGTTCTTATGAAGATTAGCTTTAAAATTAAGGAACATGAAATTTAAGCTTAATTTTCACGAAAAAATCAACCAAAAAGATTGCTGACAATCTCTAATTGATCACAAGGAACGTTCCTACTCATTAGACTTCGTTAATAATACGGAAAAGGAACTACCACTTATCTATGGATACGAAGTAAAAGACTTTGAAAATCACGGGGTTAAAATTGGTTATACAACATGTAAACCGTCTGATAAAATTCAATCAGCGATAGAAGAACGTATCCTTTCACAAGAAAAAGAATTTCGCTTTTTAGATGAAAATATAGAAAAGATAGAGGAAGTTAAAGTTATCTTTTGAGCTATTGCCATCAATGAAAAGGATGAATCATTCAAGGATTATTCACTCCATTCATTTATTAAGGAAAAAAACTTATTAAAAGAATCGCAAGCTGGCGGTGAATGGTTCATAGTTGATGAAAATAAGGATAAGTTTGAATATCTTAGCCAAATTTTTAGACAGTTTCGCGCACCTTCTCTTTTTAAAAAATAAGGAAATTTATACACCTAGAAAAGAGCAAGCCGCATGTGTTGAAGTGTTAGAAAGATACTTTCAAGCCAATCCCGAAAACGGTAGGTTTTTAATGAACTGCAAAATGCGGTTCGGTAAATGTTTCACCTTGTACTCTTATGCACAAAAGAATAACATTAACAAGATTTTGATCCTAACGTTTGTTCCAGCTGTCGAAGAATCTTTAAAGGATGATTTAAATCACATTGAAAAGAATTACAAATTTTATACCGATGATGATTTGCAAAAATCTAATTTTGATTTAAAGAATCAAAACGAGCCTTATGTAGTCTTTTTATCACTGCAAAACGTCTTAGGTAAACAAAGAATAGATGGCGCCAAAACTGATTTTGATAAAGAGCGAATTAGTAAGCTACAAGAGATTGATTTTGATTTAATTGTTTTTGATGAGTACCACTACGGAGCCAACAAAAAACGAACCCAAATCAAAGTTGAAAAAGTCAATAAAAAGATTGATAATCCAGAACAGCAAGATAATCAAGATGATGCTGAGGAAGAATTGGCAAGTACTTTTAAGTTAAAAGACATCAAAAGTAAATTTCAATTTTCATACAAACAATTAGTTTGTCTGTCAGGTACTCCTTTTTCCTCTTTAAGAAATAATGAGTTTTCCAGTAAAGATCAGGTGTTTACTTATTCTTACTTTGATGAACAGAAGGCCAAATCAGCAGAAAATCACCCACTCAAATTAGGGCAATACGGAATATTTCCGGAAATGAATATTTACTGTTTTGAATTGGCGGAAATTTTTACTGCACAAGAACAAGAAATATTCATAACTCCAGGTAAAGGTAAAAATAAGTTACCTGAAATTAGTTTTCGAAAGCTGTTTCAAACGGAAAACGTTTCTAAAGAATCAAGCAAACCAGTTTACAGATTTGTAAATGAAAACCTGGTTGAAAAGCTTATAGATTCTTTAATTGATAAACGTAAAGGTTTTAGCCACACACCTTTAAGTTGGGAAAACATTGACAAGCATAAGCATTCCTTATTAATTTTGCCAACTAGAGTTGCTTGTTTTGCGTTGGCCAATTTACTTAAAAATCACTGGTACTTTGAAAACAATGATTTTCAAATTATCAACATGTCAGAGAGCCAGTTTGGTAATGGCAAAAAAGCTTTAATTGAGCTTAATAAACACTTAGATGAAGCTAAAAAGACTAATAAAAATACTTTAACTATTACTGTAGCTAAATTAACTATTGGTATTACAGTTAAAGAGTGATCGACAGTATTTTTCTTGAAGGATTTAAAGGGTGCTGAATCTTATTTCCAAACGATTTTTAGAATCCAAACTCCATATATAAAGAACTGTAAAAATCTGAAAGAAATTTGTTATGTTTACGACTTCAACATGTATAGGTGTTTGGAAGTTACTAACGAGTATTCAAAACAAACACAAACAGACCCTAAATTTAGTGCTAGTTGATTCCAAAACTTAGACAAATTTTTACCTATCTATTTAGTTAGAGGCGATGAAATTCAAAAGACAGATCCAGAAATATTGCAGAAGTATGAGTACTTCATTATGGACAAGCGTGCATTCTCTACAAGATGAATGGATGAAAGTAACATTATCGACATAGATGTACTTTGCAATGTTGGGCAAGATGAAGATGCGCAAAAAATATTGAAAAAAATATTAGCTCATAAAAAATTTAAATCATCCAAAAAGAAGCGAGAGTTTGAAGACGTTCATTTGGAAAAAAGTCCAAAAAGTGAGGCATTTAGTGAAGGCGTTCGCTCTGGTAAGGATTACGCCGCTGAACAAGGTAATGTTTTAGAAGAACTTGAAAATTTCTGAAACTTTAACCAAGCATTAGAACAAAAAGCAAAAGCTGAATTTCAACAAAAAAACTTTGATGATAATGAATGAAACAATTACAAAAAAGGGTTTAATTTTGGAGTTAATAAGCATTTTGAAGATAAAGTTTCCATAAAGAAAATTGTTCAGAATAAAATCAAAGACTTTAAAAAAAGAAAAGGAAGAACAACTTCAACTTTTAAAAAATGAAAATGGTTATATTTCTGATGAGAATAACTTAAAAAAGCTAAGAGATTTAATTAAAGAATATGTACAAAACAGAAGCAACATACCTCCAGAATTAGACTGATTTTTTTACCGAGACCGTTTTATTCAGCTCTTTGAAAAGAGTTGTTACCAGCATTTCAAAAAGAAGACTTTAACTGAAGAACAAAAAGAGAAAAAGAAAAAAGTTAATGACAACTTTGATAGTTTAATGCATACATGGGCACGTGTTCTGCACTTTCTTTATGTATCTGTTTATAGAGAAAAAACTTTAAGAGATGTGTTGAATAAAACTGATCCCAAAGTTTTTTCAGTGGCAGTCGGAATTGAAATGGATGAGTTTTCCAAAATCCAAAAATTTTTTAAAGTGGAAAATATGAATAGACTCATCGAACAGTTTAGTTTTAGAGAAAGTGTTAAAACTGAAAACACAGAAAAATCACAGTATAGAAACAGTTTCAATTGATTCGGTTTTGGCCCCGATAGCTTAAAGAGCTAAATTTCACATTTTTAGTATGAACAAAAATAGTGGTAAAGACTACGAATCGATTCAAGGTAAGATTAAGAGTAAAGCTGAAATAGAAGATTCAGTTATATTGGAAAAAGAAACTTTTCAAGGCAAGGGTGAAGAAAAAACTAAATTAGATGAGTTGATTAGGTGAGAGGCCGAGAAAAATGACTTACTCAAGTTAATTGACAACGTCGGTAATGACGAAGTTTTCACTCCCGTAGAAACTTGTCAAAGAATGCTCGATGAGCTCTTTCCTGAAGATCATGAAGTTTGATCAAATCCCGATCTCAAGTGATTAAATCCGTGTGATAAAAACGGAGTTTTCTTTCGCGAAATTGCGCTAAGACTTGATAAAGGATTAACCAAAATTATTCCCGACGAATATGCCCGCAAGAAGCATATTATGACCAAGATGCTTTTTAGCATTGGCTTAACTAAATTTACTAGTTTGATGGTGCGTCGCACGCTTTACTATTGCATTAAAGCTAACAAGCGTAAAACCAGCGAAGATGAGGGTTGTGCAATTGCAAATGGCGCACGATTTAATAATGAATTTGGCAATGTTGTTACCCCTTATAAAGAACACTATTTTGGAAAAGAATCGAAAAGCAAAAACTGTCACTTTTGTGGGACAAATAAGAATTCTAAATATGTAAATTCTATGACTGAAGAAAAGTATGCCTATGACTTCATTCATTTAAATTCGGATGAATATGAAAACTACTTTAAAACTAATTTTGGTGTTATGAAATTTGATGTAATTATTGGAAATCCGCCTTACCAGCTATCTAATGGTAGCGGTTCTGATGGCAATGGAGCCAAAGCAATTTTTCAAGATTTTGTTTTAAAAGCAATTGACTTAGAACCAAAATATTTAGCGATGATTATCCCAGCAAAATGAATGATTTCTGCTGAAAATATTTTTTTAAATCTAAGAGATAAATTAAAGAAAAATAAAGATATAAAAGAAATTAATATTTTTTTCGACTCTAAAGACTGTTTTCCAAAGAGAGAAATAAAAGGTGGCATTTGTTACTTTATCTGGCAGAATAACTATCAAGGTAAGACTCTTATAAATACCAAATTTTCAAAGAAAGGAAAAAGCACAGAGATCGATAGTTCTAAAAGAAACTTATTTGTTTCCGTTTTAAACAACACTGAACAAATCATTTTTCGCAAAAGGATTTGACAAGACATTTACACAAAGATTGATTCCATCTCTGCTAGTGAAATGAAGAACACACACACAGAGAGAGAGCAATTTAGAAGTTAGATTTCTTAATTTCTCTGATTACGTTTCAACGCAAAAATTCTTTAATCTAAGAAGTAATTTTTTCGATAAATTTCCTTCGATTTTGAAAGCAGAAAAATTTCCCGGTTTGATTGCAATTTATGGAAGTCATTCGGTTATTAAACACGTGGATATTAAAGATATTCCGCAAATAAAAAATAATGAAGAAATTGCTAAATACAAAATTATTGTTCCACACGTTTATAGCCGTGGCAATGGAACTTTTGGAAATTACAAACCAAAAGTAAAAATTATTAAACCTAATGAAATTTGCACCGAAACTTATTTAGTGGTATATCCCACCGAAAGTAAAGTTGAGATTGAAAACGTCGCTTCTTATATGAGAACTAAATTTTTCAGATTTTTAGTTGAAATTTTTAAGGATTCTATTAACACAAATTCTCAAAATTTCAAGTTTATTCCTCTACAAGATTTTTCACGTCCTTGGAACGATAGAGAACTTTATGAAAAATACGGTTTAACACTCGAAGAACAGCAATACATCGAGGCGAACATAAGTGCTTACGAGGACTAAATTGCCGCTAATTGAATTGATTAAAGTTTTGTTTTTAAGCGTTTTAAGTACTACTTTTAATACTTCGAAAAACGCAATCAAAACAAATACTCCCAAAACTAAATTTTAGATACAAAAATAACAACTAAACACATTAAAAAGTGGTTTGCTATACTCTTTTATTACTAGTGGTTTTACACATTTTTTCACTTAACAAAAAGTTTTAAAAAACACCAAAATTGCCACCAGCTAGCAAATCCAACACATAAAGAAAAACGTTCTTTTAAGGCTTATTAGAATAAGGGTAGATTATTCATTAGTTGTTGTATGCTAGATAAATTACTCCAGAAATTTCGAGACCAAAAGAAGCCCGTCTTCCACAAAGAAGAGGGCTACTGGGAAATTAGTGCCTTGCGGAAATGGGCAGCCATTTTAATAATTGCCTTTGGAGCTGGCATTATTTACATTGTGCCCTACTTTGCCTTCTTTCAGTTTAAGACAGCAGTAGCTAATGTAACGGGAGTGGAGCCCAACAGAATCTCCCTTCTCTTAACTGCTTACGGGATTGTTTCATTGCTGTTTTATATTCCTGGTGGTTGGTTAGCTGACCGGATTTCAGCTAAAGCGCTGTTTTCGGTATCAATGTTTGGTACTGGCATTATTACCTTCTGGTACTTCTTAGTTGGTTTGAAGGGCATAGTGTGGATTACACCCAACTAGTTATTATCCACGTCCTGTATGCCTTTAGTACGGTCTTAACCTTCTGGAGTGCCTTCATTAAAAGTATTAAGTTATTGGGTCCAGCGGATGAACAAGCGAGGTTGTATTCCACATCTGAAGGATCACGCTACTTAATCCAAGTAATTACTGGTTACATTAGTGCTGGTTTGGGTGCTATTGTCCTTATTAGTACTCCCTTTCTCATTTACAAAGCACCAACTGCTGCTGACCCTAAAAACTTAGCTTCTTCCTCAGGGATCTTCTTTGCCTTACTGTTTTATGCCATTTGTTATGTGTTAGCTGGTTTACTGTCACTGTTTGTTTTACCAGGAAAGTTCTGGGAAAAGCGCAAGATTTACTTGGAAGATGAACGAATTGAATACTTTAACCCTGTGGACCAGGCTTATCACATCTTTAAATCGGAAGCAGAATTAGAAGCCTTTAAGAAACATAACACTAAGGTTTTTTGAACGAAGGTATGAACTGATACCAAAGAAACTTTGAGTTCTACAAGCGTTTGGTTAATTGTCTTTCTGATCTTCTTTATTATGAATGTGTACCAAACGATTGCTAACTTTGGTACTAACATTTTGGAATCGCTAGGACTAGAGGCAGCACTCTCCCCAACTTTAAGTAATACCTATATCTATGGGATTCCGGTGTTAGGGGCAATCTTTTCTGGTTTGATTACCAAAAAGTTAACTAAATCAACCGCTAAAAGTCTGATTGTGCAAGCCTTGTTTGTCATTTTGGGTACCTTAGCCTTTTTAGTAATAACACTAGCTTCCCCAGCCAAACCAGAAACTGGTGCATTTAACCAAGCTACCTTGTGAATAGCATTTGTGGTGATTTGCTTCATCATGTTCTTTATTGGCGCGAACCGTTCCATTTTTTGGAGTACGATCACAGAACTGAAAGTCAATAAGGAAATAGTAGGACTGGCAGTTGGTTTTATTTCGATTATTGGTTTTTCCAAAGATGTGTGGTTAAGTCCCTTACTGACTGGTACTACTAACCAGTTCATTGTCAAAAACAGTCAGGGTACCTCGTTTTACAGCCAGCAAGCCTTGGTAGCCTGAGCCATTTTTGCCCTAATTAATGCTTGTTTAGCACTACTAGTGACTTACATGATAGTGCGCAAAGTGAAGTATGGCAAAGTGTGAGTTAACCCTAAATTTAAAAAAGTCTTTGCACTAGGAGAACAACAATATGGACACTAAATTAACACCACCCTGGTTGCGTCAAGAAAATAACCTGCTCAACCCCGAAGTTACCCAACGTTTGTTTATCAACCAGGACAACATTCCGAACTTACCCACTGAAGCCCCCCAAGTTTATTTAGCCCGTTTTTTGGAATGAGTTGAACCACTAGTCTCGAAAGTAAAATTTGTGAAAGCACAAGCAGCAGTTCAGGATTACCTCAATTCTAAAGCCTGTGCACAAATTGAAACCATTATTGCAGAACGGGCGCAAAACACCCATTCGAGTTGGTTAGCTAACTGGTGGGTGCAGTACGCTTATTTAACTTCGACTGGCCCGGTTAGTCCGGAAGTTAATGCACCCTATTATTTAGAGCTCCCGACTGTTGGTTGAAGTCAAGCCGAGTTAGCAGCAGCATTGTCAGCCCAGCTATGACACATATACCAGCAAGTCCAAAAACGGCAGTTAACGAGCTTTAGTGTCAAGGACAAGCTGTTTTCACTCGATACCTTGCAATCAATCTTTGCTAGTTGCCAGATTCACCGCGCTGATGGGGATGTGTACTTTGTTAATGATCAACCTGCTAACTTTATTGTGGTGATTAAGAACAATGTCTTCTATAAGTTAGTAATAGATAACAGTGGTAGCTTAGAACAACTGCAAGCGCAACTGCAGTTAAGCTTTGTGCAAATCTTAGACAATGAGCTAAGTCACCCACCGCACTGGAACTTACTCACTGCTACCACAACCAAAGCGGAGTCACAAAGTTTACTCGATCAGTTGTGAGCGCAAAACCCAGAAATGTTGTTGGACATCTATAACAGTGCCTTTATTGTGAACTTGGACAACGTCGAATTAACGACTCCCTTACAACTGTTGCGCAATTCGACATGAACCCCTAACTTTAACCGTTGGCACGCTAAGGGGATTCAGTTGGTCATTACTAAGAACGCTCAGTTAGTCATTCTAGCGGATCACACTAGTTTTGACGGTTCTAGTGTAGCTACCCTAGCCAACATCTTTGTCTCTAAACTCCAAAAGGTCAACACTGAGGGTGCCAGCGCTTTAACACCGACCATGCTTTCCTTTCCAACAGTAGACCAGGATAAGCAAAAGTATTTTAAGCAATTGAGCAAAAATTTTAAGGACTATGTGTACAATGCCGTTATGTTTGAACTGAAATGGGACTGGTTTACGAAGCCACTTATCAAAGCCAAAGGGATTAAGAACTCCGAAGCCTTTATCCATCTCTGTTACCAAATTGCCCAGTACCAAACGAACAAAAAGTTGCAGAACACCTATGTGGCCGTGGACATGCGTCAGTACTTCCGGGGACGCACCGAGTGTCTTCGTCCTTTAAGTAAACAATCAGTTGCCTTTGTCAAACGCTACTGCAAAGATCCGAAAGGTACCTTAAAGCAGTTCCGAAAGTACTACCCAGCGATTGAAAGTCTCCACTTTGAAAAAACCCGGTTAGCGCAAAAGGGTAGTGGGGTCAACCGTCACTTGCTGGGAGCATATTTGGCTTGGAATGAACACCAAGACACAATAGCTAAACCCGCTTTATTTGAAACAAAGGCTTGAAAAACGATTGCTGCCAACCCACTGTCCACATCGAGCATTGTGGACAAGTATTTGCGCAACTTTTCCTTCGACCCCGTAGAGCCAAACGGCATTGGTATTGCCTATGCCATTGATGACACTAATTTCCGTGCCATTCTCAGTGTGTATCAGCACAACTTACAGTACCTCAAAGATTGGATGAAACACTTTGAACAAACAGTGAAAACAATCCTTAAAACTCTTAAATAAAATTACTCCTTAATGGTTAATTGAGTTAAAACGGTTGGAGATTGTTAAAATATTGGCGCTATACAAAGTAGTCTTTACGACTCGCCCGAATGCCGTTGATTCTTGATCAGGGCTGTTTTGTTAGTGAAAGTAGATTTCATAGATTGGCACAGAACATAAAACAAGGTTCCAGGCGTGAGCGCGAACAAAAACCGTTAATTAACGATAAAATTGGCTTTAATGAGTTCATTTTAATTGACGAAAATGGCTCCAATTTGGGGACGGTTAGAAGGACCGATGCCCTCAAGATGGCTGAGGAAAAACAACTCGATTTAGTCTTAATTGGTTCAAACCCAGCCAAACCCATTGTTAAGCTCTTAGACTTTGGTCGTTACACTTATGATTTAAAACGCAAAAAACGCCAGTCCAAGAAAAACCAAACCATTATCCAAATAAAGGAAGTGGTAGTGAAACCGACAATCGCTAAACATGATCTGGAATTTAAAGCTAAGCAAACGACGGGATGAGCTGAAAAGGGTTATCACGTTAAGTTTGTAGTGCGCGCTTTTGGTAGAGTAAGTACTAGGATTGAGTTAATTGAAAAAGTCTTTAATGACTTTTATCTGCTAGTAGAACCAGCAGTGGAGGTCCAAAAACCACTAACTGCTTCTTCCAAAACAATGTATTCTGCTTTGTTAGTTCCCCGTAAGAAATAAAAATTATGAAAGTAAAAAGTGCTGCAAAGAAGCGTTTTAAGCTCACTAAGTCTGGCCAAATTAAGCGCAAACACGCTTATACTTCCCACTTAGCACCGCACAAAACGACGAAGCAAAAGCGCCACTTGCGCAAGCAAGGAACTGTCAGTGCGAGTGACTTTAAACGCATTGGTAATTTAATTTAAGGAGTGAACTATGAGAATTAAAGGTGGAAAACAAACCCGCGTACGTCGCAAGAAGTGGTTAAAGCAAGCTAGTGGTAGCTTTGGTACCCGACATGCCTCTTATAAGGTGGCAAAGCAAACGGTGATCCAAGCCGCTAAGTATGCTTACCGCGATCGTCGTAACAAAAAGCGCGATTTCCGTTCGTTGTGGATCTTGCGCTTAAACGCCGCTTTGCGCGAACAGGGCATGACCTATTCGGTCTTTATCAATCTATTGAAGAAGCACAACATTGAGATTAACCGCAAAGTGTTGTCGGAACTAGCCATTAAAGAACCAAGTAAATTTAACCTAATTGTGCAAAAGGTTAAAAGTGAACAACCGAAAGCCGCTAAGCCAGCTGCTTTAGGAAACTAGCGACATTTTTAAAGGTAGTTTTACAGACCGCACTGTACTGGGCGAACTTGTGAAACTGCCTTTTTGTTTTTAGTAAGTGTAACGCTTCCACAATGCCATGGGCACTACCCTTGGGAAACTGTACACCGTACTGGTGGCTTAACTGTTCCATTTGTGCAATAAAAGCAGCACGTGCTAAGATCGAAGCAGCGGCAATTACCTTAGAATAGCTCTCACCCCTAATTAAGAAGTGGTTGGGAATAACTAATTCCTTTAACTCATTTTGCAGGCTGGTGAGTTTTTGCGTGTATTGGGTGAACAGTTTGACATTAGCAAACTCATCAATGCTAATGTCACATTGTTGTGCTGTTAGTTTGTTTTGTTGCAACAACTGGTGGTACACCTTGCAGTGCAAAAAGGTCAACATTACGTTGACATTTTTAAAGCTTTGCACTAGTTGGTTGTAAGTTTTAGCATCAACGCTTAATGTAACTTGGTCATGAACCGCTGCCTTGATTTTAGGCACTAAAGCTTTAATTTGGTAATCGTTAAATTGCTTCGAATCCCCCACACCAATTTGGTGGAGTGTATTGATTTTGTCCTTATGAATTAAAACAGCACTTACAGCAATTCCGCCAAAGCTATCACCCTTGCCAGATTCATCACTGCCAATTAGGTAAAGTGCGGTTGTGTTTGCGTGTTGCAATGGCTTATAAATTAATAAAATTATACCAACTCTACTTTCTTAGAATAAACGTTACATGGCAGAGGCAAAACGTGATTATTATGAAGTCTTAGGGCTTAGTCGGGATGCTGATGATAACGACATAAAAAAGGCCTTTCGTAAACTAGCCAAAAAGTACCACCCCGACCGCAATAAGGCACCGGATGCGGCTCAAATTTTTGCCGAAATTAACGAGGCCAATGACGTGTTGTCTAACCCCAAAAAACGTGCTAACTATGACAAATACGGCCATGATGGGGTGGACAATGAAGGTGGTTTTGCCTTTCAAGCTGATGTGTTTGATTCTTTTTTTGAGGAGATTGAAAAATCGGGTGCCTTTGATAATTTAAGTGAGTCTAACACCAAAAAGAAAGAGAAGACTAAAACTAAGAAGAAGGGTTGGTTTTGGGGCAAATCCAAACAGGAAGAATCTACCAGCGATACTACTGAATATGCCGATGTTGACGCTGGTTTAGAAGATTATCCGCCTCAATCTGATTATCCAGATGATATTCCTGATGTGGATGCCAGGATCGAAGAAGTGGATCAATCTGCCTATGCCGATGACATTCCCGATGTGGATGCGGGAATGGATTGGGAACAAAACGCAGAAGTAGCAAATTCTGCTAGTGAAATCATCCCCGATGTCGATGCTGGTTTAGCGGATGAGTTTAATACTAGTAGTGCAGCACCCCAAGCTAGTGACTGGGAAGCAATGATTGGTAATCCTGAGTACGGTTACTTTGATGCTGCTGGTGAGTGAAACTGAAAGGGCTTCTTTGATGAAGCGGGTCAATGGGTTTGACTAGAAGAGACTGAACCTAGTAGTGTCAGTAATGACGAAACTACAACAGATAGTGATGCAGTGACTGCTGCAACTACAGTAGAGGAAACGGATCAAGACAGTTGAACAGCAAACTCAGCACCAGAGCCAGTTGATGTAGAAACTCCAGTAGAATTGCAACCGGAAACTGAACCAGAACCAATTATTACCTTAAGTTCAGAGCCAGTGGAAGCACCAGCTTCTGTTGTGATTGAACCTACCCCTGAAATTGAGGAAACAACTAGTGCTGTAGAAATGGATGCTTCAGTTAAAGCAGATGTTTCTGATGAAGCTGACGCAACTAATGAACCAACTGAACAGGATACCATTTCAGAGCCAGAACAAGAAACAGACGCGGCTGCATTAGAAGAAATTAACCACACCACTGCTGATTTGGAACCAGCAGAAGTTAGTGCTACTAATGATCTAGAACAAGATGTAGTAGAGAAAGTTAATTTTTCAGAACCAGAATCAACAGTAGATACTGCTGCAACCGATCCTGTTGTAGAACAAGCTACGGAAACCTCCACCAATGGTTTCAAATTCTTTAACTTTAGTTCGTTTGTTTTAAGTGACCAAAACCCCAATCCACAAACACCCACCCACCATGAAGAAGATGCTGCAGCCCCCGAGCCAACCGTTGATGAAACTTCTGGAGAATCCACAGCCCCCGAAGTTACCATAGCTGAGTCAACTGTAGAATTGGAAACCGCAGCGGAAATTAATAACCCCGCTACCTTTGTGGAAGAATACCTCCAACCAACTAAAACTACGGTTGTTGATAAATTAGATGAACCAACTGTAGCAAAACCTACTGTTAGCGATTCTGAAAATTCAGTTGCACCAGAACCGGAATTTGTTGCTGGACCGGAACAAACCTTTAGCTGAAAACCAGCAATATCAGAAACCGAAGAAATTCCGTTAACTGCGGTAGAACCTGCTTCAGAGACTCAAACACTAATAGCAGAAGATGTAACTTCGCCAGTAACACCAACAGCCACAGCTATTCCCGCACCTTCAATTAACGCTGTACCAACTGCACCAGTAGCGGAAACCTTTGAGGCGGCGGTTGACTTCTTAAAGGAAGCTGCTAAGATTGAGGCACAACTACCACTAGTCCCAACAGTCCCAGAACAGATTGATGGAACTGATCCTAGTTTACTAACCCAGTGAGATGAGTACCTTGAAAAGACCCGCAAGCTGTTCCACAAGCTCTTTTTAACTGAGCAACTGCCTTTTATTGTTAAGACTGATCAATTTGAGATAGTTGACCCCAATTTAGACGAGCACAACGTTAACTTAATTTACACCGAACACGTACCCCAAATTTGTTTCTTGAACGAACAGCTCAAGGAAATTAGGTACACCCGTAAGTTAGTCGATCCACAAACTCAGGTAACCACCACTGAATCAATTACCCTAGAAGTTCAGCTATCACATAAATCCCAAACGGAAGCAATTGCCATCTTTAAGGGCTTTGGTCATGACTATGGTAGTGGCTGTGGTGACCTTAAGGTAGTACTTAAAGTGATCCCTAGTGTCTTTTTCCAACTGCAAGCTGATGGCTTACACACCGCTGCCCTAGTCGACCCTCTAGTGGCTTACAACGGTGGTTTAATTGATGTCTTTGGTCCAGTTAACAGCTTTAAAGTTGATATTGAAGGTGGGATTGCTAACAACGATTTAATTGAGTTTAACCAGTTAGGAGTACTAAGAACTAAGACCAAACGTGGTAGTTTATACGTCCACTTGTACTATAGTAGTGTAGCCAAAAAGGGCACTAAGACTAACTGCCAAGTGCAACAGTTCTTTGATCTAGTTCATGTGGAATACAAGCTGTTAAACTACAACTTAAAACAACTCCACAACTACCACTCGGCTCTCACTGCCCAGAAAAAAACCTTGGACCGCAAAAGCTATCAATGTTTAGCAGTGGAAAGCCATTAATTAGAATTACAATAAATTACTCATGAGCGAAAACTCACTCACAATTACCGAAATTCTCAGTTCCATCCGTACGCTTTTAGTCAAACACAATAAGGCTAAAGTAACCCAAATTGAAAAGGAACTTTTACAAGCAGTTGCTGAACTGGAAAAGAAGTTTAAGCAACAAGTACAAAACTTCAACGAGTTGCAACAAAAGATTCCTAACTTACAAAAAGTCAACGAGGAATTTCGCTTAAAAGTGGAAAAAATCCAGGAAGAAGCGCAGAAGAAAATTCAGGAAAAAGTGGCAGAACTGACCATTAAAAGTAAGGAAGAGTTGGAAAATGCAAAGAAGTATGTGATCGAAAAGTCGATTGATCAACCCTTAATTATTATTGACCAGTTCGAAATTGCACTTTCCTATGCTCAAAAGGATCCCCAAGTAAAAAACTATACCACTGGGTTTAACATGGTGTTGGATGCCTTTTCGCGCTGATTAGAAGGTTTTGGTGTTACTAAGATTGCCATTGAACCAGGGGCACAATTTGACGAAAAGGTGATGGCAGCACTGGAAGTTGTGCCTTCTGATCAACCGGCTAATACGGTGGTGAAGGTATCGAAATCGGGTTACAAACTCCACGACAAGGTCATTCGCTTTGCTTCCGTAGTGGTAAGCCAAGGTAATAAAACAGAATAATGAGCACAAATAAAAGAAGAACCATTCAGATTGAAATCACTGAAGAACACTTCAAGGATTTAGAAAAAGCCTTGGAAGCCTTGAAGGGTACCCAATTACCCTTCTCCACTACCGTAGAACAGTTTGTGGAATTAATTTTGTCCAACTATGTCGCTACTTCCAACAAGATTTCTAATTTAGCTGAAAGTGGCTTTGATGTGGCTTCTATTCAACAAGAATTAGAAAAAATTGGCAGCGCTGCAGGGGCGGATGATGCGCTCAAAAGCTTCTTGGATGAGTTGTTAAAAACATCGCAAAAAAGCTTTAGCAATACCAAAGATGGCAAAAAAAATGATGACGATAATAATTCGTCATCAAAATCATAAGAGGAAATATTAATAATTGGTGCCGAATAACAGAGTCGAACTGTTCTCTGATCCTTACCATGGATCTGTTTTGCCCCTAAACTAATTCGGCTTCAAGGATAATTCTAGTTTAAATTTGGACGGAGATGAAAAATAATTACAGCGAAGCGAATATCAAAATTCTCAAGGGGCTAGATGCTGTAAAAAAACGCCCGGGAATGTACATCGGCTCAACCGATAGTCGCGGTTTTCACCACTTGCTGTGGGAAATTTTAGACAACTGTGTCGATGAAGTTTTATCGGGCTTTGCTAATACTATTGCCGTTGTTTTACATGCCGAAAACCAGATAACTGTTAGTGACAACGGTCGTGGTATTCCCTTCGAAACACACTCCGATTCCAAGATTTCTACGATTGACACCGTCTTTACCTACCTCCATGCCGGTGGTAAGTTTGATAACGATTCTTATAAGATTGCTGGAGGGTTGCACGGGGTGGGGGCTTCGGTGGTGAATGCCTTGAGTGACCAGTTACAAGTAACGGTTAAACGTCAGGGCAAGGTGTACCGCTCGGTGTATGAAAATGGTGGTAAGATTAAGCAAAAAGCGCATTGCATTGGTAATGCCAAAATTGACGAACACGGTACCAGTGTGACTTTTCGTCCTGACCCAAAGGTCTTTAAAAAGATCCACTTTGACAGCGAACTAATTCGTGCGCGGTTAAAGGAATTAGCCTTCCTTTTCAAAAAGTTACAACTGACCTTCGTTGATGAAACCGGCAGTGGGGAAAAAGAGGTGTTCTTTACCGAAGCCGGTATTAGTCAGTACTTGGACGAACTTAACGCTGATTCCAAACAAATTGCGCAAAAGATCTTTGTTAGTGGCACTGAAGATGACATTGAACTGGAAGCCGTGTTTCAGTTTATTGATGGGGAGGATGAAAAACTACTTTCGTTTGCCAATTCGATTCGCACCAGCGAAGGGGGTAGTCATGAAGCGAGTTTTCGTCAATCTGTTGGTGATGTGATTAACAACTACTGCCGCAAGTACAACTTCCTAAAGGAGCGTGACAAGAACTTTGAGGCCAGTGAAATCCGTGAAGGCTTAAACGGCATTATTAAGGTCAATCTCCCCGAAAAGATTATTGCCTTTGAAGGGCAAACGAAGAGCAAACTGTTCTCCAAGGAAGTTAAAGCGGTAGTGCAAAAGCTAACCCAAAAGCACTTCTTCCAGTTCTTGGAACGTAACAGTGTTGATGCTAAATTAATTGTCGAAAAGCTGTTTTATAACCGCAAACTGCGCCAAGAACTCAAACAACAACGCCAAATTAAAAAGAACCTTTCTAATCCGAAGGCCGAGCGCATTTTGTTTGGTAAATTAGCACCGGCGCAGTCCAAAAAAGTGGCTGAACGCGAACTGTTTGTGGTGGAAGGTGACAGTGCTGGTGGTACGGCTAAAATGGGACGAGATCGCTTTCTACAAGCCATCTTACCGTTACGCGGTAAGGTGTTAAATGTGGAGAAAATTAACAACAAGAAGGAAGCGATTAACAACGAAGAGCTGTTAACACTCATCTTCTGTATCGGTACTGGTATTGGTAACAACTTCACCATTCGCGATCGTAAATACGACAAGATTATTATCATGACTGATGCTGACAATGACGGTGCGCACATCCAGATTTTACTGCTCACCTTCTTCTACCGTTACATGAAACCCCTAATTGAAAAAGGACACATTTACCTAGCACTTCCACCACTTTATAAGTTTGAAGGTCGTGATAAAAAAGCCCGTTATCTCTGAACCGAACAGGAATTGGAACAGTACCGTGCTAAGCATAGCCACTTTAATGTGCAACGGTACAAGGGTTTAGGGGAAATGAATGCTGATCAGCTCTGGGAAACCACGATGAACCCGATGACGCGTAAACTAATTCAGGTCAAATTGGATAACTTTATCCAAGCTGAAAAGCAAATTAATGTTTTTATGGGTGATAAAACCGAATTGCGTAAAAGCTGGATTGAAGCTAACATTAACTTTAGTAGTGAGAACTAATGGAAAAGAATAAACAAGCGTTATTACTCCAGGCCATCGAAGATGTCTTTGCCTTTAGCTTTAGTAAGTATGCTAAGTACATTATCCAAGACCGGGCTTTACCTGATCTACGTGATGGGCTTAAACCAGTTCAACGCCGCATCCTCTATGGCATGTACCAAATGGGCTTAAAACCCACTTCACCCTACAAAAAGTCAGCTCGGGCAGTCGGAGAAATTATGGGTAAGTACCACCCCCATGGCGATGCATCGATCTATGATGCGATTGTAAGGATGTCACAAGCCTGAAAGAACAACCTCACTACTATCTCCATTCACGGGAATAATGGTTCCATAGATGGTGATAACGCTGCCGCGATGCGCTATACCGAAGCGCGTTTAAGTCCGTACGGGTTTGAACTACTCAAAGACATTGAAAAGCAGTTAGTACCGTTTGTGAATAACTTTGATGACAGTGAGGTCGAACCGAGCGTGTTACCAACCCTGTTACCTAACCTCTTTATTAACGGTACCAGTGGAATAGCGGCGGGTTATGCCACTAATATTGCACCCCATAATGTCGGTGAACTGCTTGATGGTTTGAGTTACCGGATTGAAAACCCTGATTGTGATTTAAAGGCCATCTTAAAGATTGTTAAGGGACCGGATTTTCCAACTGGGGGATTAGTGTACTTTGAGCAAGAGTTGGCGAACATCTACCAAACTGGGAAGGGTAAGTTTGTGATTCAGGCCAAATATGAAACGAACACTGCGTTTGGCCAAAACCAGATTGTAATCACCGAAATCCCATACGAAACGGTGAAGGCCAATATTGTTAAACAAATTGAAGAGTTAATTAGTGACAATAAACTCAGTGCCTTGGAAAGTGTGATTGACAGCTCTGATCGTAGTGGTATTCGAATCATTATTAACCACAAGGACTTTTTGAGTGCTGACAAGATTATGGCGTTCTTGTTCAAACACACCCAACTCCAAGTAAACTTTAACCTCAACAATACGGTAATTGCCAACCGTTGTCCGGTACGGGTGGGGTTGTTAGCTTACTTTGATCAGTTCCTAGCCTTTGCCCACGAATTGATCATTAACAGCGCCAAGTATGATTTAGCATTGGCCAATAAGCGCTTGGAAATCATTAAGGGCTTAATTAAGGCGGTTTCCATGATTGATGAAATTATTCGTTTAATTCGCCGTGCTACTGACAAACAGGATGCGAAAACCAAGCTCATTGACAAGTATGCTTTTACCCTAAATCAAGCCGAAGCGATTGTGTCGTTGCGTTTGTACCAGTTAACTAACACCGACATTAAGGTCCTGTTTGCCGAACAAAAAGAGTTAGAGCAAACGATCCAAACAGCCGAACGCTTAATTGCTAAACCGCAAGCACGAAACCAGTTGTTACAAGCACAGTTTTTCCAATACAAAAAGCAGTTTAACCAACCACGACGCGCACAAATAGTTGGTTTAATTGAAAAGCAAAAGGTACAAGACAGTGACTTCATCGAACACAAGGAAGTGGGCTTACTCATTAGTCACGATGGCATCTACTTTAAGTTTGAACCAGAACAGTTAGCGAAGCACCTAGTGGAATTTAAAAGTGAACAAGACCAACTGATTTTTGGAGGCGTAGTACAAAACTCCGATTACTTTTTTATGGTGACTTCACTCGGTAACATTATTACCGTTCCCATTTACAAAACGTTAAGTAACACCAAAACCAAGATGAACGAGTTGTTGGCCAAAAAACCGATCCTAATGGAAGATGAAAAATTGGTACTAGCAGGGATTGTAAACCCAGACAAAATGGAACAACAGCTCTTGGTGTTAACGTCACAGTGCGGTATGGTCAAACGGGTCGAGTTGAGCAAAGTCATTAACACGAAGCAAATTAAGTCAAGCTGCTGCATGGCACTCCGGGAACGCGACAAACTGGTCAACGCCTTTGTTCAAACTAAAGGGGAACCTAAACTAGTTTGTCTGGTGTCATCTTCCAACAGTTTTGCTACCTTCTTAGCGGAAGAAATTCCAATTATTAGTAACAAGGGAATTGGGGTGAAGGGGATTAAACTCAAAGCTGAGGAAAAGGTACGGTTTGCAATGCCGTTACAGGATAATGATGCCTTAGTAGTGATTAACAGTGATGGTGGTGTCTATAACTTTGAAGTAGTGGAACTGGCAGTCGCTAGCCGCATGAGTGTTGGTAAAAAACTAATTCCCAAAACCAAAACCCCGGTAAGCTGTTTTGCTGCTAATAAGCACAGTGAGATTATTGGTCATCGTGGTAAAAATGGTAGTGATCTGTTTACCCTAAATGAGTTAAACCGTTTACCAAAGTCAACCGTTAGTCAAATGCGTTTGTTTAAGTGGAGCTAGTGGTTTTACCACCGTGAAGTTGCTTATCGTACTCCTCGATCTCTTCTTTGAGGGTCAACAATAACGCTTTCACCTTTTGGTAGTCCATCCGGGTGGGACCCACTATCGCTAGTTGGTGCTTACTTTCGTTGGTGGTATTAATAAGAGTAGAAGCTACCGAAACCTCTTCCCCTTCTAATCCTAACTTGTCACCAAACGTAATATTAGTAGTCTGGTTGTTTTGGTTCATAAAGGCCATTTGTTGTCAGACACTAGTATCTTCCAGTAAGTTTAAGATACGCGTCAATCTTTCCTGGTTGGCAAACTCTGGTTGTTGGGCTAGGTAATGGATGCCATAAACCTGCTTACGTGCTTGTTGGAATTCCTCAAAGTTAAACAGCTTAAACAGGATTTCGTCAATGACATACTGGTACTCGTGTACCTTGCTGCGAATAATTTCCTTTAAAACATCTAAGTGTTGGGCAATATCACAGAAACGTGTATCGATAATGCGATCATTAAAGATCCTGACACATACCATGATGTCTTCAAACTGTCTTTGGTTAGCATAGCTAATCGTTTTCTTAAAGACGTTACCACTAGCTGACACTAACAGTAGCAGTGCACAGGACTGATCGAGCATAATGAGATCAATCTTTTTTAAGACTTCATCACTACTAAAGTGGGTTAACACCACTGGTAGGTTTACCATTTCGTTGATAAACTTTACCCCCAGTTCAATAATTTCATCAATTGTTTTGTGTTTTTGCAAGATAATCGCCCTTAAGCGGGTTTTAAGGTTACTCTTATCATCATTTTTAGTGAGTAACTTGACATAGTACTGATAGCCCAACTGGGAGGGGATTCGTCCTGAAGAGATGTGGTTCTTTTTGAGATAACCCTCTTTTTCCAAAACGGCCATTTCGTTTCGGAGTGTACCGCCAGAGAGGTTTTTAAAATATTTTTTAGTTAATAATTTACTACCAACAGGTACTGGATAGGCAATATATTCGTTGATGATGGCCTTGAGAATTTGGGCTTGTCGGGTAGTTAAATTTTTCATTAGGTTATCCTAATTTTATTTTGTTAGCCGCTTGATCATCGTGAACAATACGCTCGCTTTTAAGTTAAAGAACGCACCCCATAAGCCAGGCTGCTATTTGTGAAAGGATGACGCTGGTCAAGTTTTGTATGTCGGGAAGGCTAAGGACATCTTTAAGCGGGTACACCATTACTTTAATCCAAACCGTTCCTTTAAAACACGGGCACTAGTGGAACGAATTGCGGATGTCGAATACGTCATTTTGAAAAATGAAAATGATGCTTTGAACTTAGAGGCCAAACTAATTAAACAGTACAAACCGCGCTTTAATTTAGTTTTAAAGGAAAATAATGGTTATCTCTATTTCTTTATTACCGCCAGTGTTAAACCCACCTTGGAATTGGGCCGCCGGTATGAGTTTTCCAAGAATAAGTACTTCGGTCCGTTTGCATCGAGTAAGTTTCGACTGCGTGACATTTATGACCTGTTGTTAAAGCTGTTTCCATTACGCAAGTGCGCGCCCCATGAACGGGGTCACCCCTGCTTTTACTATCAGCTTAAAATGTGCATGGGTCAGTGTATGGGCGAGGATACACCGGAACGTTACCAGACCACCGTCAAGGGAATTGAACAGTTTTTTAACCACGGTCCCGAGCAGGTTTTAAACCACTTGCAACAGCAAGAAATCAAGGCTAGTGAACAACAAAACTTTGAAGCAGCACGCCACTTCTTGGACTTACAGAAAGCCGTTTTAGAACTAGTGAACATGCAACAAACGGCCTTCATTAAAGCAAAGCAAAGCCATGACTTTATTGGTTATGTCTTTGAGAAAAATGTGTTAGCCATTACCGTCTTTGCTTACGTTGATAACCAACTAATAGGCAAAAACCAGCAGGTCTTTGAACTACCACAGGACGATGAAAAGGAAGTTGAAAGTGCTTTGGTTACCTTTATTTACCACTATTATTCCACCAATAAGATACCCAAAACCCTTACAGTTTCTTTAAGCGAAGAGAATTTGAGCTTACTGGCCAACAGTCTTAAAATTAATGTTACGCAACCAAAAAACGGCGAGCAAAAATCCATTTTGCAAACCGTGATTGACAACGCCCGTTATGCCTTAAACACCAAGTGAACTGGGTTTATCAATAACCTTAACCGTGCCGAAGTGCACCAGCAATTAGCGCAGCTTTTACAAGTTCCTTCGATTCAAAGTCTGGAAATTATTGACATCTCCTTTTATGACAAAGACCACGTGGTGGGGGCGATGTTACGGTACGAAAATGGCAAACGGATGAAAGCGTTATCACGCCGTTACAACATTAACATTGACCATAAGGGTGACACTAACTATATGGCTGATGTGGTGTACCGCCGGATTATTTCGTCCATCCAAACCCATAAACAGCTACCTTTAAGTGATCTTTTAATTGTCGATGGTGGGATTGCCCAAATTAATACGGTAACCAAGGTGTTTGCCTCTTTCCCTAACGTTACCCAACCGATCATTATTGGCTTAGCGAAAAACACCCGTCACCAAACTGACCACATTGTGTTAACGGATAACACTACCATTAACATTGACAAAAATACGCCTTTGTTTGCTTATCTTACCACCATTCAAGAAGAGGTCGATAGCTTTGCCAAACACAATGCCTTTAAGCGAGTTAGTCGCGCTCGCTTCCAAAATCCACTCTTACAAATTGAAGGGGTTGGGCGAAAAACTGTCCAAATATTACTTGATAATTTTCAAACAATGCAAAACATTGAAGCTGCTTCTTTGAGCGAATTATCACAGTTTATTCCTGCTAATTTAGCACAAAAAATTAAAACTTTTCTAAAGGAAAATGACTAAAGTATTAGTGTTATCAGACACCCATGGCTATAACGATAGGTGACTAGCCGTTATGAAGTTGCACAACCCTGATGTAGTAATTCATGCTGGTGATCATTTAACTACTAAAAAGTTTATGGATCAAAATGCCACTTTTTGGGTTGCTGGTAATCATGATGTTGTTGGCGAAGAGATTCAAATGTTTGAGTTAGAGGGAATCCAGTTTGTTTTAATGCACGGCCATCAAGCGCCCCGTCATGATCTCAAGCAGTGGTATAAAATGCTGGTTGATCAAGCTAAAAGCTATCTTTGTGATGTTTTAATTGTGGGTCACAGTCACATAGAGCACTATGAAACTATTGATGGGATCCAGGTTATTAACCCCGGTTCTCTAGAAATTCCCCGTAATCCGAGAAAGCTACCTACCTACTGTAATTTAAACTTAAGTCAGGGCAGGATAAGTGATTTAACGTTCCATTTTCCTCGGGATTAAGTCAAATAAATAAAAAAAGCCATCTACCTAACAAGCAGAAGGCTAAAAAATACTTTTTTTAGCGATATTGAAATTAACAGAAAGATCTGTTAGCAAATGTAAGGAACAAGCTTATGCAAACTTTCAATATGTGACGTGTTGAAAAACCTTTAAGAGTATGCATCAATTGGGAGTAGGAAAGCAGGCTTTCAACACAAGTTTTCGTTTTCCTGCTCCCACTAAATAAATTGGGAAAGGTTTTCAAAGTCGCCAAAAAGATTAAATATTTTTGGAATTTTGAATTTTTAGTTGCTTCTAATTGTTAATAGAGATTTACTTTCAAAAAAACTTTTTGGTTTATTTTTGGTGTCTTTACTAACTTAAAGCAATTTAGTTAGTGTAAAGACTCATAATGTTTAAATTAAAAATTAACAACTTCCAATTAGGTTTCAAACTCGTTCAGTGACCAGTGACGAACCACCTTCTTAAACATTTTTACGTTTTTCCAATCAATAATAAAGGAGGTCTTGCCATTAAACGCATAATTTCACTTGCTTTGTTTAAGAAAAGACTTAACAAAGATAAGATTAATAATTGTCATGTTTGGGAAGAAGAGTTACCTGATGGTAGCTACGACATGGGATTTAATGGCAATTTCAACCATATGGAAAAACGAAAAAGTGGTTATGTTACCCAAAAGCAGTTTAGCGAGTTCAAAGATGCCAACAATCAGCGTCTCATAAAGATTGAAACTACTTTGGCTATCCAAGGCGAACAAATCAACAAATTGACTCAAACTGTTGAAAAGCAAGGCGAACAAATCAATCAATTAGTTCAAGTTGTGCTTCTTCAGGGCGAGCAAATTAGAGAACTTCAAGTGGAGCAAAAAGCACAAAGACAAGAGTTTAATGCCCGCATGGATCGTTTGGAAAATCTTTTGGTGGAAAGTATAGAATCTACCAATAAGCGCTTCGACTCTATGGAAAGACGTTTAGACTCTATGGATAGTCGTCTTGATTCTATGGAAAATCGCTTAGATTCAATGGAAGGTCGTCTTGATTCTATGGAAAATCGCTTAGATTCAATGGAAGGTCGTCTTGATTCTGTTGAAGGACGCTTAGACTCTATGGAAACTCGTTTAGACTCTATGGAAACTCGCCTGGACAAAGTCGATCCGCCCAAATAGTTTTTAGTTAAAAAATAAGGGTATCAGCAGTTAGTCTTAAAGATTAGTTGCTGGTATTTTTATTCATTTAATGCTTTCTAAAAACTTTTTGTCAATTTTGTTGGCACTTCTAAATTGCTAGTTCAATTTATTAAGTGGAGGAGCAAAATGCAAATCTTTAATTTTTGAAATTGATCAGTAAAAAATCTGATCACCGTTGGTAGCATTGCTGCAAATAGTAGTTATGTAATTTGCAAACAGAAAGGAATTTTTCAATATGAGTAATACAAAAAAATTAAGAATAACTTCAATCGAAGAAACAATAGAAATAATAGTTAATTCAAAACTATTAGCTTTTGGTTTTTACCCAGTTGGTTTAGAAGATGGAACTTACGAACTTAATGTAAGGATTATTTTTAAAGAAAAAACTGCAGATTCTGAGAAGAGTAGCAGTGTACATCCTTTACAAAAGGAGTATTATGTAAGGGAATTTTTGAGATTTCTTGAGCGCCATAAAGATTCTCTGGCGCAACAACTATTAGTAGTAGAAGACAATTAAATAATTTCTCCCAAACTGGATTGACTCATCCTCACCGCCACCGCCTCCCTCGCGGCGGGACTCACCGTAGTGGGACACTTCACAAGTACCACCACGACGCTCAAGCGCCAGCAATTTAGCTACACCCGCCCTGACGAGGTCGCGCTGCGCCACGCCAATGCCATCAACCCGCGCTTAACCCCGTGAACGTATCGTAACACGAGCTTTTCCTCCCTCCCCCTCACGGGTGAAAACCCCGGGGCGTGGGCCTTAGTGCGCGACAACAGCGCTAAGGGCATCACTGCCGGCAGTGGCAGTCAACAAACCACGTATGAACCTGCGCGAACCGAAGCGGCTTTGACCGCATCAACCACCTTTGCGTTACGCCGGTATGACCTCGCCGGGCGCGCCTTATACGACCTCGACTTTTCGAAGTTAAACCCACAAACGCCCACGCGCGACCAAACCGGGCAGATCACCTTTAACCCCTTTGGCGGCTTTGATTTGAGTGGGGCTGCACCCCAACAGTGAAACGAGGTCAAAAACAAGGTCCCCGTCGAGGTGGCGCAAGACCCCTCCAATCCCTACCGGTTTGCCGTTTTACTCGTGCCGCGCAGCGTGGTGTACTATGAGCAGTTGCAAAGGGGGTTGGGCTTACCACAGCAGCGAACCGAGAATGGTCAAAATACTTCCACCACCGGGGCAATGTTTGGCTTGAAGGTGAAGAACGCCGAGGCGGACACCGCGAAGAGCAATGAAAAACTCCAGGGCGCTGAGGCCACTGGTTCTTCAACCACATCTGGATCTGGCCAATCCACCCAACGTGGGGGTTCGTCAGGGGACACCAAAGTCAAGGCGTTGCAGGTGGCGGTGAAAAAGAAATCCGGGAGTCAGGGCAACTCCGGTGACCAAGGCACCGAACAGGTGGAACTTGAATCTAATGATTTAGCCAACGCCCCGATTAAACGGGGCTCCAATCCAGCAAGTCCAACTCAAGGCAGCCGACTTCGGCACCACCCCATCCAGTTCGGAATCTGGTCAATCAGGCACCCCCACCCCCTGAAGGCCGTGGCTTGCGACCGAGCAAATTCACAAGGACCTCCCCAAATGATCCGCCTCGATCCTCATTCTGTACGATGCGCCTTATGCCTTTAATTGCTCTTGTTAATTTAGTTAAGTTTTTGTTTGCACAAAATTAAATTTTCTGTTTAATTAACTATCAATATTTTGCTGCTTTAAAATTTAAAGCAACTTTTAAACAGTAAGTTGTATCTTTTCACAAAGATTTAGTTCGATAAATTATTGGTAACAAGAGGGATGGAAACAACCGCAAAAAGTATTGATTGCAAAGAATTAGGCCTTTCTGTAACGACGAAGCAACAACTGCATAAATTAACACTTGCTACTATTTGACTTTGGGATTCTTTGTTTTTTGTTTTCTCTTACTTTTGTTTATAACGCTTACTTATACGATCTATTTTGAGCAACACATTTTGGGAAGTGATAAACCACTTCCTTTTTGGTGATACATTAAAAATCATGTGGTTCCCACCTTTGGCGTCTTAACTTTAGCTTGTTGGTGTGCGTTGATTGGGATTAACTTTTGGGTTTACAAACACACTTCACAAACAGAGCTGTTTCACAAATCAATTAGGTGACTTAAATGAGGTATTTTATTACCGCAAATTGCGCTAGCTAACTCCCTTTTCATTAGACAGAAACTAAGGCTAATGGAAGGCGAAGAACAGTTGAATAACAACAAAATAGAACAGAAACTAAAAGCTTTATTTATTAGTTTCTTTACTATTTGAGCAGTACAATTGCTTTTAGGTTTTTTCTTTTGGTTGCCGTTCTATGGTCTCTTTTTTTGATATATATACCAAAGCATCGATTGTAGAAGCTGTACGTTTTTCTTTTTTGAATACCTTTCAATTAAAGGAACTTGGTTTACCGATTAAAGAAATTGCTGTACCATTAGGCACATTAGTCTTTCTTTTTGTTGTTATTATTACCTTAATTCCGTTGTTAATTATTGGTAATTTAATTTGGACTAACTTGCGTTTAATAGAACGGGAAAATACACAGCAGTACCAACTCGTTTTTGGTTACAGTTTGATAGTTAGTGATATTGTTGGTTTTGCTATTGTTTTCTTCGGTGCCATTTTAGGTACAAATTTAAAAAGTGTTGAATTATTCATAGCATTGGGTTGAATGATGATGTTAGGATCATTAATTGCATTGGGCACTACTGCTAACTTAGTGAGCTCAATTTATCTCTACATAAAACTAGCATTGAAGAGATAGACACACTTCTCCCCCACGCTCACGCGCTTCTCCACCGGGTTCAACCTCGCGGGGTCGGTTCTCGACCAGGTGTTGGATTATGTGCCCTGGATTGGGAATGGGTACAGGTATGGGAATAACCACCGGGGCGTGGATGATATAACCGCGCCTAAAACCGGCGCGGGGTCGTCCAGCGGAACTAGTACGAACACAAGTGGTTCGCGTTCCTTTCTCCCGACGTTCTCGAATGTCGGCGTTGGCCTCAAAGCCAACGTCCAAGCCACGCTTGGTGGCAGTCAGAAGACGACTACAGGCGGCTCCAAATGACCCACCCTCGACCAAGCCAACCTCCTGCTCTGAACCGGCGCGGGGTGAAGGAATGATAAGAACGGACAAAGTGACGAAAACCACACCACCTTTAAAAGCGCTACGGGCAGTGGCCAGCAGGGTGGTTCTTCAGGTACAAATACCTCAGCGGGGAATCCCGACTCGTTAAAGCAGGATAAAATCACCAAGGAAAGCTCGGGTAACCTCAAAACGGAGGACGGCGCTGCCAACTCTAACGAAAACAAAGACAACACTAATTATCAAAATTTACCCGCTTATGGGAAAGTTAGTGGGTGGATAACACCTGATTATTAATAGGCTTTTTACTTTCCAAAAGTTTTAAAGTTGTTTTGGCACTTTCAAACTTTAAAGACCATTTAAAAGGCATAAAAGGATAGTTATGTCTTTAAGAAATATTTTGCGATTCAGTTCGCTTTTGGTTTTATTTGGTTTTGAGTCTTTTCTAGTTAATGCTCAAATCTTTTTGCAAAGTTTGTTGTCACTTCTAAATAACCTACTCAATGTATTTAGTGGAGGACAAAAAATGCTTTTCATAAATAAATTGATTAAATCAGTAAAGAGTCTGATATCAGTTGGCAGCACTGCTGCAAGTAGTAGTTTTCTCAACCTCTTTCAGAAAGGAGTTATTGCTTAATGAAAGAAAAAACTCCCTTTAAAAACGAAAAAGAATTCCATGACATGGTGAAAAAGACCAAAAAGGGCACTTTTTCAGGTTGATACATCGTTAATCCAGAGAACAACAGTGTGGAATTCTCTGGCAATTTCAATAGACAATTTAAACTTAACAAACCGGTTATACCAGTGAACACGGAATACGTAACGCGTAAAGAGTTCAACGAGTACAAAGACTCAAACGACCAACGACTCATAAAGATTGAAACCACTTTGGCTGCCCAAGGTGAACAAATAAGAATCCAGGGTGAGCAAATTAAAGAACTTCAAATCGAGCAAAAAGCTCAGAGCGAAACCTTAAAGCTAATCTTGCAAACTTTGCAAAAGATGAGCGATCGCTTGGACAAGATGGATGTTCGCCTAGATAAACTAGAGTCTAAGTAGATATTATTCAAAATAAAGAATACCAGCAATTAGTCTTTAAAAGATAGTTGTTGGTATTTTTATTCATTTAACACTTTCTAAAAACTTTTTGCAATTTTGTTGGCACTTCTCAATAAGCCGGTCAATTTATTTAGTGGAGGAACAAAATGCAAATCTTTAATTTTTGAAATTGATCAGCAAAGCGTCTGATCCCTGTTGGCAACCGAGTTGCAAGTGTTAGTTATTTTTTAAAAAATCAAAAGAAAAAAGAAAGGAACATTTAAACATGAAAAAAGTTAAAGCATTAAGGAATCTTTTTTTAGAAAAAGCCAGTGAAAAACTTGAATACCCCAAAATTGCTTCAATGGTAGCTTACTTTGATCCAAAGGAAGATGGTACTTGTGAATTCATTATGAAAATTAAAACTGTCAATGAAGTTGCTGACACTGAGACGACAAATAAGGAAAAGCCTTTAGGCACTTGAAAGAGTTAGTTGATTTTTTGGTCTAAAAAGACATAAATTTACCAACAGTAAGTGTTAAAACTTGCTGTTGGTATTTTTATTCCCCCACGCTTTCCCGCTTCTCCACCGGGTTCAACCTCGCGGGGGCACTCCTCGACCAGGTGTTGGATTATGTGCCCTGGATTGGGAATGGGTACAGGTATGGGAATAACCACCGGGGGAGCAACAGTAGTACGAGTGGGGTAACGACCCAGGGACAATCCCAAAATGCATCCAGTAACGAACCCGCACCGACGTTTTCGAATGTCGGCGTTGGTCTCAAAGCCAATGTCAACGGCACCTTAAGTGGTTCGCGAACTACGCCTAATCAGCAAGGCACTCCCTGGTTAACCCTCGACCAAGCCAACCTCCAGCTCTGAACGGGCGCGGGGTGAAGGAATGATAAGAACGGACAAAGTGACGAAAACTACACCAATTTCGCGAGTGCTAAGGGCAGTACGAACCAGCAGGGTTCAACAACGGGTGGATCAGCGGGCAACCCCGACTCGTTAAAGCAGGATAAGGCTGATAAAAGTGGTGATTCGGTAACGGTTGCAGAAGCCACATCGGGCGACAACTTGACGAATTACACCAACCTCCCCCCAACATCACCCCCACATCCGACTGACCGAACGCGCTGTCATTCACCAACAAGAACAACGCCCAGCGGGTGCAGCTGTTCCTGCGCGGCCTGTTGGGCAGCATCCCGGTGTTGGTCAATAAGAGTGGGCAAGATGATAACAGTAAGTTTAATTCCACCGACCAAAAATGGTCTTACACCGAATTAAAGTCGGATCAAACCAAGCTCAACCTCCCCGCTTATGGTGAGGTGAATGGGTTGTTGAATCCGGCGTTGGTGGAAACCTATTTTGGGACGACGCGTACGTCTAGCACTGCGAACCAAAACAGTACAACCGTCCCCGGTATCGGTTTTAAAATTCCCGAACAAAATAATGATTCAAAGGCTGTGCTGATCACCCCCGGGTTGGCTTGAACGCCCCAGGACGTCGGTAACCTCGTTGTCAGTGGTACCAGCTTGAGCTTCCAGCTCGGCGGGTGGTTAGTTACGGTTCACGGACTTTATCAAACCCCGCGCGGGTTATTTGGGCCTCCAGTTAAGTGGCCTGGATGCCAGTGACAGCGACCAAAGGGAGTTAATTTGGGCCAAGCGGCCCTGAGCGGCATTTCGTGGCAGTTGGGTCAACCGGTTGGGACGGGTCGAGAGTGTGTGGGATTTGAAGGGGGTGTGGGCGGATCAAGCTCACAGCGCGGTGTCCGAGTCGCAAGCAGCTACAAGTAGTACCACCACCACCGCAACAGGGGACACCTTACCGGAGCACCCGAATGCCCTCGCGTACCAAATTAGCTCCACCGACAAGGATTCGTACAAGGCTTCCACTCAAGGCTCCGGTCAAACCAATTCCCAAAACACCTCCCCCTACCTGCATTTGATTAAACCTAAAAAAGTTACTGCTTCCGACAAGTTAGACGACGATCTTAAAAACCTGTTGGACCCCAACGAGGTTCGGGTGAAGCTGCGCCAAAGCTTTGGTACAGACCATTCCACCCAACCCCAACCCCAACCCCTCAAAACAACGACACCGGTGTTTGGGACGAATAGTGGTAACCTCGGTAGTGTGCTTAGTGGTGGGGGTACCACGCAGGACTCAAGCACCACCAATCAACTGTCACCCGTTCAACGGGTGAGTGGGTGGCTTGTGGGGCAGTTACCAAGCACGAGTGACGGAAACACCTCCTCCACCAACAACCTCGCGCCTAATACTAATACGGGGAATGAGGTGGTGGGGGTGGGAGATTTGTCTAAGCGAGCTTCCATTGAATCAAGTCGTTTGTGAATAGCATTAAAACCTTAGCTAACGCTAACTTTGGGCGATTTAATCCACTTAGTTTTGAACTCCTTAAATTTGTGTTTTTCTTGACTTTGCTAAAAAAACAATTCAAAGAGGCTTGCAATGAACAGGTCAGCACTGCTAAATTGGTGAGTGCAAGAACTAAATAAAAGTAATTATTTTGTAATTTTTCGGCGGTCTTGCCATACATCAATTAAATGCTTGATCAAAACCAAGGTGTCAAAATTTAGTGCACAATTGCTAAGAGCGCCTGTATATTTAGAGTAAAACTACTTAAATTGCTAAAAATTAACCGTTTTTGAATTAGTTTTAGGGTCAAAATTAGGCCAATTTCCCGCTTTCTTTTTTTAAAAAATCTCTACTTTTTACCCCCTAAATAACCTAACAACTACACAATTAATTCCCAATTAATGTAAATTTATCTAAATTGTTCACCGTAGTTAGTTCAATTTAGGTAAAGATGAAAGGGTTTTCTTGCTCCAGACCGGGCTATCTTACTGGTCTCTTGCTCCTCGCTGTAGCCCCTATTCTCACTGCTTGTACCCGCGACTATACTACCAAGAACGAATTTCAATTAACAACAGCTCAACAGGCCAAATTAAAGCCAGCCACCATCGAATATTGGCGTGATGGTGATACGCCTGAGATCAATTACGCAAGCGAAGAGCGCCGAAAGGAAGCTGAACAAAAATCTAAAGAAAACGCTAAGAAAGAAGACAAAAAAGAGGAAAAGAAAACTGAAGATTCCCAAGATTCTTCTTCTGCATCCACGCAAGTTCGTAGTAGTAAACACGGTCTCCGCATCTATGGAATAGATACACCCGAAAAACATGTAAGTAGCAAAGGCGATAGCACCGGTGATGAAAAAATCGAAGCCGAAAAGGCCTCTAACTATGCTGAAAAGCTAATCCCTAAAGGCAGTACTGTTTGGGTTTGATCACTCAACACTTACAGCTATGATCGTGAAGTGGGCGCTTTATTCTTTAAGTCTAACCCTAAGCAAACCTTCTTCCAATCCTTTGAAGTAGCTATGGTTGAGGCTGGTCATGCTATTCCAATTGCTGGGACTGGTTTAAATTTAATCGCTGATCCGGAATTAAGTGCTGATGATCCTTTAAGTGTCATTGGTCTACAGTTAGCCAACGCTGCCAATAAGGCTTACAACGCTAAAATTAACATTTGGAGCCATGACACTGATGGTTATCGTTCCTTGACTGCTGTTTACAAGTTAAGGGGCGCTGATATTAGTTGAACCCGTTTCCTAGATGAAGCCAATGGATATTCCTCTGCTAGTGCTGGAACCGGTGCTAGTTTGTACCAACTATGAGATCAACGTCAAGCCAAATTAGCCCAAAAGGGTAGTTAATGAAAGCAGTTTAAACCAGCAATATGGAAAAGATACAAGCGGAAAAATCCCAAAGTGCAATTGAGTTTAAGAATATTGTAGTCGACTTTGGTGAGTCAATCGCAATTGATAACATTAACCTCACGGTTAAAAAGAAGGAGTTAGTTACCTTACTAGGTCCTTCTGGGTGTGGGAAAACAACTTCGCTTTCCGTGATTGCGGGTCTTATCGCCCCTACTAGTGGTCAGGTTTTATTTAATGGTTATGATGTCACAAAAAAGCCACCACAACAACGCAAACTGGGATTAGTGTTCCAAAACTATGCCTTGTATCCGCACATGAGTGTGTTTGAAAACATTGTCTTCCCACTCTACAGTGATACGTCTTGACGCGAAGCCATCTTTGAAAAAAATACCTGAGCGCAACACGACATTAATTGCTTGATCTTAAAGGCTAATGGTGCTACTAGTGAGGAGCTGGCAGAGCTCAATCGTTTAATGCAACAACGAATCGATGAGCCCAAACGAATGGCTTACCAGATTAATGATTTGATGGTAAGTGTGTTTCAAAAACAGAGTGAACTGGAAGCTAACCTAAAGTTAATTCCCCGCAAAAAACAGTTTGCCATTATTTCACTCAGCAAGGAAACACTAAGTCAAATAAGGGATGTTGAAACTAAAGCCAAGGCTGCATTGGAAACCGCTGATAGTGCTGAAGTGGAGCAGACCATTAAAAGTGAACTTAAGCAAAAACTAAGTGAAATTAAGGCTAATTACCACGATGAAAAGGCCAACATTAAAGCTTACTGATGGGAAATGTTGGCCAATATTAAGACCGAATTAAAAACCGAAAAAACTGCCATTAAGCAAACTAATGACTATGCCAAATTGAAGGAATTGAAGTGAAAGATCCACTTTGAACCCTTGAATTTAAAGAAGCAGTACCGCAGTTACTTTAAACAACTAAAAGCCAAATACAGCTTAAAAGATGGCAATTTAACTGAAAGCGAATTAAGTCAAATTGAGGAGTTGCAAAAACGGATTGTTTCGCTCAAAGATTTCATTAACCGCACTGCTAAAGAAGTAGCTGAAAAACTGGAAATTACTAAGATTCTCCACAAGCGCCCCGCTAATATTTCTGGGGGTCAACAACAACGGGTAGCTATTGCTAGAGCAATTGTTAGAAGACCTAAGGTTCTACTTATGGACGAACCGTTGTCCAACTTAGATGCGAAGTTGCGGGTACAAACACGGCAATGGATCCGTAAGTTCCAGCAAGACCTGCAAATTACTACTGTCTTTGTGACTCACGATCAGGAAGAGGCAATGAGTATTAGCGACACGATTGTCTGCATGTCAACTGGTAAGGTACAGCAAATAGGCTCCCCTTCCGAGTTGTACTTAAAACCGGCCAATGAGTTCGTCGCTACCTTTTTGGGTAGCCCGGAAATGAATATCGTCAACGCTACGGTTAAAGCTGGACAGCTGTTGTGAAACGAAAATCCCCTAGTTAAAACAAAGTTTGATTTACCTGATGGTGCCATTCGGGTTGGTTTCCGTTATGATGAGGTAACGGCTCCCAAAAACGATGGTAGTCCCGTATTTAGCGGTACCCTTATAAGTGTGGAAAACCTAGGGAAACACATGGTTGGGGTTGTGGAGAGCAATGGGGTACAGCTCAATGTGCGCTTAGAATTGAGCCACCAGTTTGAAGTAGGTAATGCAGTTAAGTTTACGATTAAGCCTAATGGCTTACACTTCTTTGATCCCCAAACAACGCAACGGGTGGAGGTAAAACATGTTTAAGTGATTGTTAAAGCACCGCTCTAAACCAACGCCATTGGAGCTGGGAATCTTTGACCAAAAGGTTTCCTTTTGAAAACCATTTTTGCTGTTTTGCCCAGCTTTATTAACTACCTTTCTCTTTACTTTAGTACCGTTCTTCCTTACGTTACAGAAAGGGTTTAGTCACAATGAGGACATCTACCGGGTTGATTCCCAACAGTTTGGCTTTCAAACTTTCGCTAACCTTTTTAGTGAGTCCAACTTTATTTTGGGCTTGCGCAATTCCTTTTTGTACTCGATTATTTCCCTACCACTGACAATTGTGCTAGCAATTATTATTTCTAGTGCCATTGTCTTTGTGTACCGGAAGTTAGCACGGGGCTTTTGACAAACAGTATTCTTTTTACCCTATGTAACTTCCGGAGTAGCGGTTTCGATTGCTTTTATTTATATTTTGGACTCCTCTTCGGGAATCTTAAACAACATCTTTCATGTCAACATTAAGTGGTTAGATTCGGGGGAACGTGATACCTTTAATGCCTTATGGGGAATTCTTATCTTTGGAATTTGGAAGAACATGGCTTTTAATGTGCTGGTAATATCAACCGCCATGTTAAGTGTGGATCCTACTTTATATAAGGTAGCTAACTTAGATGGTGCAAAACCAATTAGGCAGTTCTTTAAGATTACCTTACCTTCAATCCGCCCAACCTTAATTTTTCTGTTGACCCTGTTAATTTTGGGCGGAATGCAGGTGTTTCCAATTTCGTTGTTTAACGGGAATGATAGTGAAGCTGTAACCAATGGGGGTTCGACCATCTTGCTTTACATCTTCCAAAAGATTCGTGATCAGAATAACAATTTTGCTGGTGCTGCTACCTTGGTGTTGTTCATCTTGGGGGTTTGTTATGGGTTAGTGTTGCGCAATGGTTTTCGCTTAATTGAATGAGCGCAGTGAAAGATAAAGAGACATTATGTTCAAACAAAGCTTAATCTGGTATAACCGTTACGTCCAAAAACGGAAGAAGGGTTTAGATCTCACCATTAAAGATCGATCTTGATCGAATGTATTGTTGGGTTTAATCTTTAAAACGGTGGTGTTGTGCTTCTTTGGTTTAATGGTCATCTTTCCCTTTTATTTGATGCTGGTGGTAGCACTCACCAGTGATGAAGTGGTCTTAAACATTCGTGAACCAATTCTCAAATCCGATGGGTGGCACTTCGAGAACTTCCGCCGTGTTTTGGAAGATGGTAAATACCTTAATGCCATTTGGATTAACAGCTTAGTAACAATTTTGTCGATTATCCTACGGCTTTTCTTTACCGTTAGCATGGGCTATGCCTTTTCGTTAAAAAAGTGAAAGCTCAAGAAGCTGTTTTGGTTTATCTTTTTGGCAGTGTTAATTTTACCCGAATCGGCCTTGCTAATTGGTCAGTATCGTGTGGTGGTAGTTGCCAATTGAAATCAACCAGAAAAACCAGCGATTATCTTGGGCTTAACAATGCCATTTGTCGCTTCTGTGTTCTCTGGGTTTATGTTCCGTACTGCCTTTGAAGCAATTCCACCACGAATTAAAGAATCGGCCTTTGTTGATGGTTGTACAGGGTTGAGGTACTTCCTCAAAATTGCCTTTCCAATGGTACGTAGTACCACTTGAACGGTAAGTATTTTAACGGCCTTTGCGGCGTGAAATTCTTACTTATGACCGTTATTGTTACTAACAAACAGACCCGATCTCAACATTAACCTGTGGGTGTTGGCGCAAGGGACTGATGGCAATGCCGGGCAAAGTGACGAACAAATCCGGGTGTTGTTGAATTTAAAGATGGCAGCAGCCATTTTAGCAATTCTGCCAATGTTTATTGTTTACTTCTTGTTTAGGAAAAGGATTATGAAAGCCGTGGGATCACGTGCCAATACGATTAAAGGATAGTTATGGACAGCTTAAAGCATTTTTGACAACAACTAGTAATGTGATCAAAAACTGTTACCTTTTGAATCATAGTAGCAGTAGGGATTGCTTTTTGTGCCTTTGCCATTTGGGGCATTATTGCTTGCATTGAAACGATTATCAAAGAAGGGGCGTTGTTGTAAGTTAGCTTTCTTAGCTTTTTTATAAAAACACCAGATTGATATTTAGCCTTTTTGAGTTAGCTGTTCTTACTGCTTTTGGCGGTTGCTATACAGAATGAAGGTCGTTTGTAAAGGAACGTAAACTTTGCTAACTTAAAGATCTCTTTTTGGGTTTTAGGTGTTTTATTTTTGTTACATAAAATGAAGAAAAGGTTAGGAAACTACTTGCAATCAAAAAAAGCGTTTTAGGTAAAAAAAGTGCTTTGTTTCATAACCACCCTTCGAGGTGGTTTTTTATTTGTTTTATAGCTTTGTATACGAATTCACTCTATAGATATAGGGTGCTTTTATTTTCTGGCAAGCTGAAAAGGGTAAAAAATACCAAAAGTTAGCAGTTAAGAAACCACTAAGAATTGGTATTTTGGTTTTACTAAATACTATTTGGGTGGATCGATTTTGTCTAAGCGAGTTTCCATTGAATCCAAGCGATTTTCCATAGAGTCTAAACGTCCTTCAACAGAATCAAGGCGACCTTCAACAGAATCAAGGCGACCTTCTATTGAATCCAAGCGATTTTCCATAGAATCAAGACGACTATCCATAGAGTCTAGACGACCTTCAATAGAGTCGAAACGTTTATTGGTGGATTCTATACTTTCAACCAAAAGGTTTTCCAAACGATCCATGCGGGCGTTAAACTCTTGTCTTTGCGCTTTTTGCTCCACTTGAAGTTCTCTAATTTGCTCGCCCTGTTTTTCAACAATTTGAGTTAATTTGTTGATTTGCTCACCGTGAAGAAGCACAACTTGAACTAAGGCTTCTAACTTTTGGTTAATGCGTGCCTCCGAATCTCTAAGGTCAGCTTTTGTAGCAAATGTGTCATGCAGTTCAACCATTAGATCTTGCTTGAACTCTTTTAATTTCTGATCAAGCTGTTTATGCGTTACGTACTTTGTGCTTGGTTTTTTAGTCACGACTTCAAAATCTCCATTAAAACTTAAATGATAAGTGGATTTACCCTTATTCCATCTACGTTTAATGATAAGGTTAGTTATTTTATCTTTGTTTAAAGATTTTTTAAATTCTTCTATCGATATATCTTCTTCTCATGGCTTCTTTTCCATTAAGCAATACCTCCTTTCTTGTTGTGGTTTAAAAAACTACTATTTACAACAGTGTTTAAGTAAGGGTTCCCATTTAATCAGGAATTGAGTTTCTCTAGTGTAATCATTTCTATGATGCATACACTAACTAAATGGATTTACTTATGCAAAAACGCCAAAAATAAACCAAAAAGTTTTTTGAGCAAAATTGCATGAATAAAAATACCAACAGCGATTTAGCGATTGGTATCTTCATTTTTTATCTAAAACTATTTGGGTGGTTCAATTTTGTCTAAGCGACCTTCAATAGAATCAAGGCGTTTGTTAATAGCAGCAAGGATTTTAGCTAACATTTCAAAGCCACTGTCCACTTTAGCTTCAAGTTTGTCGACTTTTTCTTCTAATTTGTCCACTTTAGCTTCAAGTTTGTCGACTTTTTCTTCTAATTTGTCCACTTTAGCTTCTAGCTTATCGACTTTCTCTTCTAATTTATCGACCTTAACTTCCAGCTTATCAACCTTATTTTCAATCTTTGTAAGTCGTTGGTCGTTTGAGTCTTTGTACTCGTTAAACTCTTTGCGCGTTACGTATTCCGTGTTCACTGGTATAACCGGTTTGTTAAGTTTAAATTGTCTATTGAAATTGCCAGAGAATTCCACACTTTTGTTTTCTGGATTAATGATGTATCAGCCCGAAAAAGTGCCCTTTTTGGTCTTTTTCATCATCTCGTTAAATTCCTTTTCGTTATAAAACGGAATTTTCTCTTTCATTAATAAGCGACTCCTTTCTTTTCAGTGAAATTTAAAAAGTAACTACTACTTGCAACTCAGTTGCCAACAGGGATCAGACCATTTACTGATCAATTTAAAAAATAAAAGATTTGCATTTTGCTCCTCCACTAAATAAATTGACCGGCTTATTGAGAAGTGCCAACAAAATTGCAAAAAATTTCAAATGAATAAAAATACCAATAGCAATTTAGCCATTGGTATTTTTTAGTCTACAGTTATTTAGGCGGATCGATTTTGTCTAAACGAGTTTCTATTACGTCGAGGCGTTTACCAAAATCAGTCAAAGTGTTTCCGATTGATTCTAGGCTTTTAAGCACAAGACTTTCAAAGCGATCCATGCGGGCGTTCATTGCTTCACCCTGGGTTTTGATTTGTTGACTCTGAACCATAACAACTTCAGTTAGTCGATCAACTTTGTCCTCAACAACTTGAATCCTTTGACCCTGACTCTTTAACTCAGTTTCAATCTTTGTAAGTCTTTGGTCGTTTGAGTCTTTGTACTCGTTAAATTCTTTACGCGTTACGTATTCTGTGTTCACTGGTATAACTGGTTTGTTAAGTTTAAATTGTCTGTTGAAACTGCCAGAGAATTCCACACTGTTGTTATTTTTATTGATGATGTATCAACCCGAAAAAGTGCCCTTTTTGGTCTTTTTCACCATGTCATGGAATTCTTTTTCGTTATAAAACGGAATTTTCTCTTTCATTAAGCAATAACTCCTTTCTGATTAAAGCGGTTCAAAAAACTACTACTTACAACTCTTTGTCATTGGACTTTTCAAAATTTGATCATTGTTTTAATGCTCCTTACTAACTAAATTGACCCGCTTCATAAAAACTGCCAACCAAGTTCAAAAAAGTTTTTAAGCTATTTTTGCACCAAAATGGCGTTGTCAGTCAAACTACGACAACAACAGTTGCTGTTTGATTCTTTAAACTTAAACAGAATTAGCACCAATGAATAGCCAAGTACACCGCAAGGGCTCTATTGCAGAAGCAGTTAGTGCCATCCAAGCTCATGATAAGATCGTGATCTTCCACCACATTCGTCCTGATGGCGATTGTTTGGGCGCACAACACGGCTTAGCGCGTTTAATCCAAACTAACTTTCCCCACAAGCAGGTCTTCTGTGTTGGTGATCCCAAACACAACTTTCCCTGATTGGAGATGGTTTTCACTCCAAAGGAACAGATTACCCCGGAGTTAATGCAACAAGCCTTAGCCGTTATTGTTGATGCCAACTATAAGGAACGGATTGAGTGCCGGGACTTATTAGACCAAAACCAGTTTAAGGCAGTATTGCGGATTGACCACCACCCCAATGAGGACGATCTCAATACGACCCATAACTTCGTTGATGCGTCTTACATTGCCGCGGCTGAACAAGTGGTAGATCTAGCGGTGCAGGCCAAATGGAAGCTTAGCCCCCCAGCGGCTACGGCGCTGTATCTAGGTATTTATACAGATAGTAATAGGTTTCTATATAGTAATACATCATGAAGAACACTATATCTAGGATCTATGCTATATAGAGCTCAAGCTAATATAGCTAAGATCCATGATGAGTTAAACCACACTTCCTTAAAGGACATCCAGTTTAAACAATATGTCTTTAAAAACTTTCAGACCTTTCAGAATGTTATTTACTTTGTGGCCGATAAGAAGTTCCAAAAGAAATTAAAGGTAACACCCTTAGAATGTGCACGGGTAAATATCCTAGCTAACATTGAACAATTCCACATTTGGCTGTTCTTTATAGAAGAGGGTAAGAACCACTATCGGGTCGAATTCCGTAGTAACGGAATTAACGTACGCGAAGTAGCTTTAAAGTATGGTGGCGGGGGTCATATTCAGGCCAGCGGTGCAGTTCTTAAAAGCAAGCGCGACATAATTCGTGTAGTTCAAGATTGCCAAAAGCAAATTGCTGTATAATTTTTAACAACTATGCACCAAACCAAAAAAACTGCCTTGTCCAAGTCCACTTGGATTCTCATCCTCACCGCCACCGCCTCCCTCGCGACGGGACTCACCGTAGTGGGACACTTCACAAGTACCACCACGACGCTCAAGCGCCAGCAATTTAGCTACACCCGCCCTGACGAGGTCGCGCTGCGCCACACCAATGCCATCAACCCGCGCTTAACCCCGTGAACGTATCGTAACACGAGCTTTTCCTCCCTCCCCCTCACGGGTGAAAATCCCGGGGCGTGGGCCTTAGTGCGCGACAACAGCGCTAAGGGCATCACTGCCGGCAGTGGCAGTCAACAAACCACGTATGATCCCACCCGAACCGAAGCGGCTTTGACCGCATCAACCACCTTTGCGTTACGCCGGTATGACCTCGCCGGGCGCGCCTTATACGACCTCGATTTTTCGAAGTTAAACCCGCAAACGCCCACGCGCGACCAAACCGGGCAGATCACCTTTAACCCCTTTGGCGGCTTTGGTTTGAGTGGGGCTGCACCCCAACAGTGAAACGAGGTCAAAAACAAGGTCCCCGTCGAGGTGGCGCAAGACCCCTCCAATCCCTACCGGTTTGCCGTTTTACTCGTGCCGCGCAGCGTGGTGTACTATGAGCAGTTGCAAAGGGGGTTGGGCTTACCACAGCAGCGAACCGAGAGTGGTCAAAATACTTCCACCACCGGGGCAATGTTTGGCTTGAAGGTGAAGAACGCCGAGGCGGACACCGCGAAGAGCAATGAAAAACTCCAGGGCGCTGAGGCCACTGGTTCTTCAACCACATCTGGATCTGGCCAATCCACCCAACGTGGGGGTTCGTCAGGGGACACCAAAGTCAAGGCTTTAAAAATAGAGGTGAAAAAGAAATCGGACTCGGAGGACAATGGTCAGCTGCAGTTAGAAAAAAATGATCTCGCCAACGCTCCCATTAAGCGGAGCGAGGAGTCGGGTCAGTCCGTCCAACTCAAGGCGGACGATTTTGGTACTGCCCTTTCCAGTTCGGGATCAGGCGGCAACTCCAATCCCGGTTCCCCCACCCCCTGAAGGCCGTGGCTTGCGACTGAGCAAATTCACAAGGACCTCCCCAAATGATCCGCCTCGATCCTGATTCTGTACGATGCGCCTTATGCGCGCAACCGTACCGCCATTGACCGCGTTGATCACTTGGATCCCAAGGCCATGACCGCGAACTATCCGCCCAGTTGAAGAACGCCCAAGTGAAACCACCACGGTTTGTGGGACTGAAAGGCGCGCGATGTTTTGCTCCAAACCACCGGGTTCTTCAACCCGCGCCGCCACCCCGAGTGGTTTGATGGCGGGCAGACGGTCGCGGATAACGAAAAGACCGGGTTTGATGTGGATAACTCTGAAAACACCAAGCAGGGCTTTCAAAAGGAAGCTGACTCCGACAAGTCGGCCCCGATCGCCCTCCCGTTTGAAGCGTACTTCGCCAACATTGGCAACCTCACCTGGTTCGGGCAAGCGCTTTTGGTGTTTGGTGGCAATGGCCATGTTACCAAGTCGGCCCACACCGCGCCTTTGAGTATAGGTGTCTTTAGGGTGCGCTATAATGCAACTGGTACCAGTGCTACTGTAACTGGTTGACCATATGCCTTACTGTTCTCAGGCATGGTCAACAAACAAACTGACGGGTTAAAGGATCTACCCTTTAACAATAACCGCTGGTTTGAATATGTACCACGGATGGCAGTTGCTGGCGCTAAGTTCGTTGGTAGGGAACTCGTTTTAGCGGGTACCATTACCATGGGTGATACCGCTACCGTACCTCGCTTACTGTACGATGAACTTGAAAGCAACCTGAACTTAGTAGCGCAAGGCCAAGGTCTTTTACGCGAAGACTTGCAACTCTTCACACCCTACGGATGAGCCAATCGTCCGGATTTACCAATCGGGGCTTGAAGTAGTAGTAGTAGTAGTAGTAGTCACAACGCACCCTACTACTTCCACAATAACCCCGATTGACAAGACCGTCCAATCCAAAATGTGGTTGATGCCTTTATTAAGCCCTGAGAGGACAAGAACGGTAAGGATGATGCCAAATACATCTACCCTTACCGTTACAGTGGCATGTGAGCTTGACAGGTATACAACTGGTCCAATAAGCTCACTGACCAACCATTAAGTGCTGACTTTGTCAATGAGAATGCTTACCAACCAAACTCCTTGTTTGCTGCTATTCTCAATCCGGAATTGTTAGCAGCTCTTCCCGACAAGGTTAAATACGGTAAGGAAAACGAGTTTGCTGCTAACGAGTACGAGCGCTTTAACCAGAAGTTAACGGTAGCTCCTACCCAAGGAACAAACTGATCCCACTTCTCCCCCACGCTTTCCCGTTTCTCCACCGGGTTCAACCTTGTGGGGTCGGTGCTCGACCAGGTGTTGGATTATGTGCCCTGGATTGGGAATGGGTACAGGTATGGCAATAACCACCGGGGCGTGGATGATATAACCGCGCCTCAAACCAGCGCGGGGTCGTCCAGCGGAATTAGTACGAACACAAGTGGTTCGCGTTCCTTTCTCCCGACGTTTTCCAACATCGGCGTCGGCCTCAAAGCGAATGTCCAAGCCACCCTCGGGGGCAGTCAGACGATGATTACAGGCGGTTCGCCTCGAAGAACCCTCGACCAAGCCAACCTCCAGCTCTGAACGGGGGCGGGGTGAAGGAATGATAAGGCTTCAAGTGGACAAAGTGACGAAAACCACACCAAGTTCACGAGCGCTACGGGGATGGACCAGCAGGGACAATCAGGTACCTCCGCGGGGAATCCCGACTCGTTAAAGCAGGATAATATTAGTAAGAGTGGGGATAGTTTAACCACGCAGGACGGCAATGCGATCGATCAACAAGAGGCCACCAACTACACCAACCTCCCCCCCAACCTCACCCCCACCGCTGATTGACCGAACGCGCTGTCATTCACCAACAAGAACAACGCGCAGCGCGCCCAGCTCTTCCTCCGCGGCTTGTTGGGCAGCATCCCGGTGTTGGTGAATCGAAGTGGGTCCGATTCCAACAAATTCCAAGCCACCGACCAAAAATGGTCCTACACCGACTTACATTCGGACCAAACCAAACTGAACCTCCCCGCTTACGGTGAGGTGAATGGGTTGTTGAATCCGGCGTTGGTGGAAACCTATTTTGGGAACACGCGAGCGGGTGGTTCGGGGTCCAACACGACCAGTTCACCCGGTATCGGTTTTAAAATTCCCGAACAAAATAATGATTCCAAAGCCACCCTGATCACCCCCGGGTTGGCTTGAACGCCCCAGGACGTCGGTAACCTCGTTGTCAGTGGCACCACGGTGAGCTTCCAGCTCGGCGGGTGGCTGGTCACCTTCACGGACTTTGTCAAACCCCGCGCGGGTTACCTCGGTCTCCAGTTAACGGGCTTGGATGCAAGTGATGCGACGCAGCGCGCCCTCATTTGGGCCCCCCGGCCCTGAGCGGCCTTTCGTGGCAGTTGGGTCAACCGGTTGGGCCGCGTGGAGAGTGTGTGGGATTTGAAGGGGGTGTGGGCGGATCAAGCTCAGTCCGACTCGCAAGGATCTACCACCACCGCAACAAGGAACGCCTTACCGGAGCACCCGAATGCTTTGGCCTTTCAGGTGAGTGTGGTGGAAGCGAGTGCTTACAAGCCAAACACGAGCTCCGGCCAAACCCAATCCACTAACAGTTCCCCCTACCTGCACTTGGTGAAGCCTAAGAAAGTTACCCAATCCGACAAGTTAGACGACGATCTTAAAAACCTGTTGGACCCCAACCAGGTTCGCACCAAGCTGCGCCAAAGCTTTGGTACAGACCATTCCACCCAGCCCCAGCCCCAATCGCTCAAAACAACGACACCGGTATTTGGGACGAGTAGTGGTAACCTCAGTAGTGTGCTTAGTGGTGGGGGTGCTGGAGGGGGTTCTTCAGGCTCAGGTCAATCTGGCGTGGATCTCTCCCCCGTTGAAAAAGTGAGTGGGTGGCTTGTGGGGCAGTTACCAAGCACGAGTGACGGAAACACCTCCTCCACCAACAACCTCGCGCCTAATACTAATACGGGGAATGATGTGGTGGGGGTTGGTCGACTTTCTGAAAGCAACGCCGCAAAGATGAATGACGATGTTGATGGTATTGTACGCACCCCACTCGCTGAACTGTTAGATGGGGAAGGACAAACAGCTGACACTGGTCCACAAAGCGTGAAGTTCAAGTCTCCTGACCAAATTGACTTCAACCGCTTGTTTACCCACCCAGTCACCGATCTGTTTGATCCGGTAACTATGTTGGTGTATGACCAGTACATACCGCTGTTTATTGATATCCCAGCAAGTGTGAACCCTAAAATGGTTCGTTTAAAGGTCTTGAGCTTTGACACCAACGAACAGAGCTTAGGTCTCCGCTTAGAGTTCTTTAAACCTGATCAAGATACCCAACCAAACAACAACGTTCAGGTCAATCCGAATAACGGTGACTTCTTACCACTGTTAACGGCCTCCAGTCAAGGTCCCCAAACCTTGTTTAGTCCGTTTAACCAGTGACCTGATTACGTGTTGCCGTTAGCGATCACTGTACCTATTGTTGTGATTGTGCTCAGTGTTACCTTAGGACTTGCCATTGGAATCCCAATGCACAAGAACAAACAGGCCTTGAAGGCTGGGTTTGCGCTATCAAACCAAAAGGTTGATGTGTTGACCAAAGCGGTTGGTAGTGTCTTTAAGGAAATCATTAACCGCACAGGTATCAGTCAAGCGCCAAAACGCTTGAAACAAACCAGTGCGGCTAAACCAGGAGCACCCCGCCCACCAGTACCACCAAAGCCAGGGGCTCCTAAGCCACCAGTGCAACCACCTAAAAAACCCGCTTAGTATTTATGAAATCGAAGCTAAAGTTAAAACGTTATTTACTGTTTTTACCACTTTTACCGCTAGGGACGTTGTCACTAGCCAACACCTACCTCCTCCAAGACCACAACACCCTCACCCCCTACACGCCCTTTACGACACCGCTCAATGGGGGGCTGGATGTCGTGCGCGCCGCCCATTTACACCCCTCATACGAACTCGTGGACTGAAAGCGGGTGGGGGATACCAAGTTGGTGGCGCTGGTCCGCTCAGCGTTGGTCAGGGTGAAATTCCAGGACACAACGAGTTCGGATCAAAGTAATACCAACCAAAATGCCTTGAGTTTTGATACCCAAGAATCACAGAAGGCACTTAATGGCTCGCAGAGTGGATCTTCTGACACTTCCGGGTCTAACTCCCAAGACTTCGCCAGCTATGTCCTCATCTTTAAAGCCGCGCCCAGGGCCACGTGGGTGTTTGAACGCAAGATTAAGTTGGCGTTGCCCTACGTTAAGCAGGAAAGTCAGGGTTCCGGCGATCAAGGTTCCAATGGTAAGGGCTCCCTCTACAAAACCCTCCAAGACCTCCTCGTCGAACAACCCGTGACCCCTTACACCCCGAATGCGGGGTTAGCCCGGGTGAATGGGGTTGCTCAGGATACGGTTCATTTTGGTTCGGGTCAAGAATCGAGTTGGAATTCCCAACGTTCCCAAAAAGGCCTTAAAAACAACCCCGGACCCAAAGCCGTCACCGGCTTTAAGCTCGATAAGGGCCGCGCGTACCGGAAGCTGAATGAAAGTTGACCGGTGTATGAACCCCTGGATTCGACCAAGGAGGGGAAGGGGAAGGATGAGAGCTCTTGGAAAAATTCGGAAAAAACAACAGCGGAAAATGATGCCCCGTTGGTGGGGATGGTTGGAAGTGGTGCGGCTGGAAGTGCTTCTAGTTTACAAGGCAATGGCTCGAACAGTTCGGGGTTAAAATCGCTCTTGAGATCAGCACCTGTCAGTGTTCCACCAAGCAGTACAAGTAATCAAACTTTAAGCTTATCTAACCCCGCTCCTGTGGGCCCACAAGCGGTTGTAAGCCAACCCGCGGGGGGTGCTACGGCAGCAGTGTCCGTCAATCGCACAGCGAGTGACACCGCCACCTTTAGCAAGTACCTCAACACCGCCCAGGCCTTGCACCAGATGGGGGTGATTGTTCCGGGGTTGGAAAAATGAGGTGGTAACAACGGTACGGGTGTAGTGGCTAGCCGACAGGATGCTACTTCCACTAACCTGCCCCATGCGGCAGGTGCTTCCCAAACGGGTTTGGGAACTGGTTCGCCCCGCGAACCAGCTTTAACCGCAACGTCACAGCGTGCCGTCACGGTGGTTGCTGGCCCCCTTCGTGCGGGCAATAGCAGTGAAACTGATGCCCTACCGAATGTCATCACCCAGCTCTATCATACTTCAACCGCCCAACTCGCTTACTTAAATGGCCAGATCGTTGTGATGGGTTCCGACCGGGTACCGAGTCTTTGGTATTGAGTTGTCGGGGAGGACCAGGAATCGGGCAAAGCGACCTGATGAGCGAAAACCGAGCTCAACTGGGGCACCGACAAGCAGAAGCAGTTTGTCGAAAACCAGTTGGGGTTTAAAGATGACTCAAATTCGGATTCCAAAAATTCGAATTTGAAGGCCCAAGGCCTCACCCAACCCGCCTACCTCATCGCCGGTCTTGACGTTGTGGCCGACCACCTCGTCTTTGCGGCCTTTAAAGCGGGCGCGGTGGGGTATGATATGACGACTGATTCGAGCGCTTCGACCTACAACCAAGCACTCGCCTGGTCGACCACGGCCGGGTTGGACAGTGATGGGGGGTACAAGGCCTTGGTGGAAAACACGGCCGGGCTCAACGGCCCGATTAATGGCTTGTTTACCCTGCTCGACACCTTTGCGTATGTGACCCCCGTGAGTGGGATGAAAGGGGGGAGTCAGAATAATGAAGAAGTGCAAACGACTTACCCGGTCAAGTCCGACCAAAAGGCCACCGCCAAAATTGCCTCCTTAATTAATGCCAGCCCACTCAACAGTTATGGGGATGATGGGGTGACCGTGTTTGATGCCCTGGGCCTTAACTTTAACTTTAAGTTGAACGAGGAGCGCTTGCCATCGCGCACCGACCAACTGCTTGTGTATGGGATTGTAAACGAAAGTGAACTGAAGTCCGCACGGGAAAATGCCCAGTCGACCTCCGATGATAATTCAAACACCAAAGTCAAGTGAACCAACACCGCCTCGCACTACCTCCCCGTGCCGTATTACTACAGTGCCAATTTCCCCGAAGCGGGTAACAGAAGGCGAGCGGAGCAGCGGAATGGGGTGAAGATTAGCACCTTGGAATCGCAAGCCACTGATGGCTTTGCCAACTCGTTACTTAACTTTGGTACCGGTCTTAAAGCCGGTGTTGACCCAGCTCCAGTAGCACGGGGTCATAAACCGAACTATAGTGCAGTACTACTAGTGCGTGGTGGCGTTGTAAGGTTAAACTTTAACCCCGATACTGATAAACTGTTGGATTCTACTGACAAAAACAGTGAACCTATCTCCTTCTCCTATACCCCATTTGGGTCTGCTGAAAGTGCCGTAGACCTCACCACGTTGAAGGATGTGACCTATATTGCTGAAAGTGGTCTGTGGTTCTATACCTTTGACAATGGTGAAAAACCAACGTACGATGGTAAACAACAACAGGTCAAAAACCGCAAGGGTTATGCTGTGATTACCGTATCACGTACCGGAATTGAATTTAACGAGGACGCTAATACCACAACCTTAAGCCAAGCCCCAGCTGCTTTGGCTGTCCAAAACGGGATTGCTTCCAGTCAGGACGACCTCACAGGCATCCTACCGTTATCCGATGAGTTCTCCGCTGTGATTACCAAGGATCAAACATGGACCGGTAAGGTTGATATCTATAAGAACACCAACGGGTTGTTTGAAAAGGATGATCAGCTATCGGAAAACGTGAAGAGGCGTGACAACGGTTTGGTCCCTATTTACAACGAAGGTATCGTCGATATTTGGGGCAGAGTGGATTTTGCTGCCAACAGTGTTTTGCAAGCGCGTAACCTCACTGATAAAACGGTTGATGAGGTGATCAATAACCCCGATATCCTCCAAAGCTTCTTTAAGTTTACCCCAGCCTTTGATAACCAAAGAGCAATGCTAGTGGGGGAAAAGACATCGGATACTACCTTAACGGTTAAACCGAAGATTGAGTACTTGGATGGTAACTTCTATGGTGAGGATTCCAAGATTGCTGGAATTCCGCTCAACATTGATTTCCCTTCCCGGATTTTTGCTGGCTTTGCTGCTTTACCGTCCTGGGTCATTCCGGTATCAGTCGGTTCATCGGTGGGCATTCTCTTAATCCTGCTCATCTTAGGCCTTGGTATTGGAATTCCAATGTATAAGGTCCGCAAGCTTCAAGACTCCAGCTTTGTTGATGTGTTTAAAAAGGTGGATACGTTGACAACCGCTGTGGGTAGCGTGTACAAGAAGATTATCACCCAAACGAGTGTGATCAAAAAAGCTCCTAGTGCGTTGAAAGCTGCTAATAACGCTGCTCCTAAAGCACCAGTTAAACCAGCTGCTCCAACAGCTCCAAGACCACCAGTCCAACCACCTAAAAAGGCTTAACGATTAAGCCCCAACAGAAAGAAGCACAACTGTAAAAAGGTTGTGTTTTTTTTCATATTTACAACTTTTTGCTTTTTTGTTGGCACATTACCTTCCTAGTGCAATTTAGAAGGTGTATGAGTCATAAAGATTTTAATGGGCTTCAAGCCCCTCAGTTGTTAAGTTCGAGTTCCCCTGTTGCAAAGAAACAAAGTAGTCATAAACTCAGGCATGCCTTAAAGCACGCCCGTTACTTAAACCATAGTAGTAAGCGTACCCTCAAGCATGCCTTAGAGCTGCATGAAGACAACCAGGTCTTACTGGAAAAAGAAGGTAGTCCTAACTTCCAGGACTGGCTTTCTAAGCAACCTGGGGTCAATAAAACCAGCCTTAAGTACAACAAAAGCTTGGGTAGTTGAATCTCTAAGGAAAGTAAACCGAAAAAGCGTTTTCCACCGTACTTTACGTACAAGGGCAGTAAAACTACTCCAGAAGAGGCCAAAGCCTTGCAGCAAATGAAACAATCCCAAAAGCGCTTCTTTCACGAAAATATGCACAGCTTTCTCAATGAAGTTGCACATAACCCAATGATCCAGCGCTTTAAGCAAAAGCAGGCCAAACGGGCTGCTAATACGCGACAACGCACTTACAAATACCGTCAGTAACAATGCACAAGATTAAACACTATAAACCCTTAAAGCGCAAGACCTGGTTAAACAAGGTTTCCAAGCAACGCCGCGAGCGCATGTCCGCTAAGTGCTATCAACCACCTAAGAGAGAAGTGATGCAGCCAACTTGAAAGAGTTAGCACCACCTGATCCCACTTCTCCCCCACGCTTTCCCGTTTCTCCACCGGGTTCAACCTTGTGGGGTCTGTTCTGGACCAGGTGTTGGATTATATTCCCTGGATTGGGAATGGGTACAGGTATGGGAATAACCACCGGGGCGTGAATGATATAACCGCGCCTCAAACCAGCGCGGGGTCGTCCAGCGGAACTAGTACGAACACAAGTGGTTCGCGTTCCTTTTTCCCGACGTTTTCCAACATCGGCGTTGGCCTCAAAGCGAATGTCCAAGCCACCCTCGGGGACAGTCAGACGACGACTGTGGGCGGTTCGCCTTGAAGAACCCTCGACCAAGCAAACCTCCAGCTCTGAACGGGGGCGGGGTGAAGGAATGATAAGGCTTCAAGTGGACAAAGTGACGAAAACCACACCACCTTTAAAAGCGCTACGGGGATGGGCCAGCAGGGACAATCAGGTACCTCCGCGGGGAATCCAGACTCGTTAAAGCAGGATAAGATTAGTAAGAGTGGGGATAGTTTAACCACGCAGGACGGCAATGCGACCGGTCAACAAGAGGCCACTAACTACACCAACCTCCCCCCCAACCTCACCCCCACCGCTGATTGACCGAACGCGCTGTCATTCACCAACAAGAACAACGCGCATCGCGCCCAGCTCTTCCTTCGCGGCTTGTTGGGCAGCATCCCGGTGTTGGTGAATCGAAGTGGGTCCGATTCCAACAAATTCCAAGCCACCGACCAAAAATGGTCCTACACCGACTTACAGTCGGACCAAACCAAACTGAACCTCCCCGCTTACGGTGAGGTGAATGGGTTGTTGAATCCGGCGTTGGTGGAAACCTATTTTGGGAACACGCGAGCGGGTGGTTCGGGGTCCAACACGACCAGTTCACCCGGTATCGGTTTTAAAATTCCCGAACAAAATAATGATTCCAAAGCCACCCTGATCACCCCCGGGTTGGCTTGAACGCCCCAGGACGTCGGTAACCTCGTTGTCAGTGGCACCAGCTTGAGCTTCCAGCTCGGCGGGTGGCTGGTCAGCTTCACGGACTTTATCAAACCCCGCGCGGGTTATTTGGGCCTCCAGTTAAGTGGCCTGGATGCCAGTGACAGCGACCAAAGGGAGTTAATTTGGGCCAAGCGGCCCTGAGCGGCCTTTCGTGGCAGTTGGGTCAACCGGCTGGGCCGCGTGGAGAGTGTGTGGGATTTAAAGGGGGTGTGGGCGGATCAAGCTCAGTTGGCCGCGCAAGCAGCTACAAGTGAAGCTTCCGGGTCAGCTTTGGCACCTCACCCGAATGCTTTGGCGTTTCAGGTGAGTGTGGTGGAAGCGAGTGCTTACAGCTCTTCAACCTCAAGTTCGGGTTCCGGGTCAAGTTCAAACACCTCCCCCTACCTCCATTTGATTAAACCTAAGAAAGTCGAAAGCACGACCCAACTCGACCAGGGCTTAAAAAACCTGTTGGACCCCAACCAGGTTCGCACCAAGCTGCGCCAAAGCTTTGGTACAGACCATTCCACCCAGCCCCAATCGCTCAAAACAACGACACCGGTATTTGGGACGAGTAGTGGTAACATTGGCAGTGTGCTTAGTGGTGGGGGTGCTGGAGGGGGTTCTTCAGGCTCAGGTCAATCTGGTGTGGACCTCTCCCCCGTTGAACGGGTGAGTGGTCACTAACTATTAATAGGAACTTTTACTTTCAAAAAAGTTTTGAAGTTATTTTGGTGTCTTTACCAACTTTAAGCAATTTAGTGAACGAATACCCAAAAAGTATGAAATTAGTTAAATTAGCGAGGCTTCACCTCTTTTTAAAACAAGTTCAATTGCAACTTGTTCCTTTGTTTAGCCAAAACTTTTTGCAATTTTGTTGGCAGCTTTAAATAAACCGATCAATTTATTTAGCAATGACACAAAAATTAATATGTTGAATTTTTTAAATGAATCAACAATGGTATTGATCCCCGTTGGCAGCAGTGCTGCAAGTATTAGTTACTTTCCAAACCTTACAAAGAAAGGAGGTGATTTTTAATGTCTTACTCACCTTCACTTAAGGAAATTATTGCTATTTTGCAAAAATACACTTCGAAAAACTACCAGTCAAATTGCAAAACTAGACCCGATGGTAAGCTTGAATTATCATTAAATGGAGTTTTTGAAGAAATCGTGAAAACACCCGGTAAAACTAGGTACGTAACCCATAAACAGCTTGATCAGAAATTAAAAGATTTCAAGCAAGATCTGATGGTTGAGTTGCACGACACGTTTGCTACTAAAACTGATCTTAAAGAATCGGAAGCACGCATTAACCAAAAGTTAGAAGCACTAATTCAAATTGTTATGGTTCAGGGTGAGCAGATAAAAGTTCACGGCGAGCAAATTAACAAACTGACTCAAGCTGTTGAAAAACAGGGCGAGAAAATTGAAGCTCAAGGACAACAAATTCAAAAAGTTAATGAAACTTTGAATTTTGTAGTTGAAAGCCTTGGATCTATCCACAAAAGACTCGATTCAATGGAAGGCCGCTTGGACTCAATGGAAAACCGTCTTGACAAACTAGAGTCCAAGTAGTTCTAATGGAAATAAAAATTCGAGCAGTTAGTCTTAGAGATTAGTTGCTGGTATTTTTATTCACTTTGAAGAGAAAAATCATTTGTTTAAACTCAAAAAGGTCAATAATTTTTAAAAAATGCTGAGGTACAACCACCTTTTAAAAATTCCTTAGATATAAATCGCTAAACTATCTTTATTTATTTTGTATGTGCCAAATTATTTTTGACACATCAAATCAAATATTATTTTTTATCATTCCCCTAAATTTCAGGGGTATTTGAGTATGCGATTTAAATATGGTGCAGTTGCTTTTAGTGGCTTTTTAGGTCTAGGTGATGTTGGCTAGTTGTGGTGCTAAAGGGCGTTTTGACCAGGTTGATGATGGCAAAATTAAGCTAGCTTCTTCACTTACCGGTAAGCGCGATGTGATACTGCAAGAAGTGCTCAACAAATATAACAGTCGCAAAGCCAAGGACGATTATCCGATTGAAATAACCAAAATAGCCGGTAGCTATGATGGTGGTCGATCTGATCTGCAAACGCGGTTGAGTGTCAAGGATAAAACAACCTTTTACAACATGATCTTGAATTATTCTGACACCATTTCTACTTTAGGTCGCAGTAACATGGAACTACCATTAGATAGCGTTGACGTATCACAGTTTTCAGAAAACTTCTTGAGCTTTAATGACCGCATAAGTGGTATTTCCAGAAAAGGCATTTACGGCATTCCGGTATCAATGTCAACTGATATCCTAGTAATCAACGGGCCGGTATTGCACTATATTTTGAATAGTGCAAAAAAGAAAGATGGTGCAGTAACTAAGAAAAACGCAAGTAATTCCAATGGTAACGAAGGAACGTTAACAGTAAATAACGATCAACAAACCACTGAACTATGAAAAAAGATTGAAGAAGCCGCTAAAACTAACGGTAAAACTACCCAAGAACAAACAAAAAGAGATGCAAAACAAAGTACATCCTTAATCCAACTAAAAGAAGGATCTGCAAATACAACCGAAGGCAATGCTTCAGAAAGTGATAAAGAAATCAAAAAATCTTGAGGCAATTACCAAGAAGTAGATGGTGGTCTTAAGGGCTATACCTTCAAAGCAAGCGTCTTTGAAAACTAAAGTGAAATGGTTGATTTTGCCACTAGAGTAGCGAAATCATTTAAGGATAAAGTTAGTGGTATCGATAATAAAAAAGGCACTGACATCCAAGGTGTTTTAGGTCTAGACAGTACACCAAACGTTTTGTTCGCTTCAGTATTTGCAGCTGGTGGTGGTAGCTATGACAACTTCTTTTACAAGGTAGAAAATGGCCGGGCTGATTTCCGTAATTTTGCAAATAAAGGTACTTCTTACAAAAACTTAGAGAAGGTTTACAACGATTACAAGAATTTAATCGGTAGCAACGGTCTTTTTGCTAGCAAAGATGGCTCTTACTCATCTAACTTTGAAAAGTTCCACCAGTTAGCTTTTTATGTAGGTTCTAGCTCTGGTTATAACTATGCTTTTGCTGATGAAACTGCTAAACGGTTAAAGTTTGGTGATAGTGCCATTGAGTATCCTAACGATACGCTTGAAATTAAGGCACCGACAAATAATAGTCAAAGTGGTGATGGGAATGGTGGCACCAATAACGACAATCTCTTAGGTACTTTTGACATTAGGGAAAAGAGTAACGGCAAAAAAGGTGAGAGCAACGGTAAACAAGGAAATGGTCAAGATAAAAAGACAATTTCGCTTTATAAAGATTCGATTCCTAAAGAAAAAACTGAAGGCACTGATGCAATTCTAATTAGCGATAATCAACTCATTAATCAATTACAAACTGCCGCTAAAACTAGTAGTTCACAAAATAAAGCTAACACTGCAGCTATAACTTTTAAGCAAAGCAATAAAAGTGAAACTGACCAAAGTACAACTAGTCAGGTTATCGGCTACACAACAACTGCTTCATTGAAAGCTGATAAGAAAAATATCTTTGATGTGAAGAAGCTAAATAACGAAAAGTCTGAGCGCAAAATCATTGTCGGTGCCACGGTGGAAACCTTAAATCAAGCAAACACATTGCAAGCTAATGAAGCCATTATTAAACCAGCACCTGGTAAGTACCAAAGTACTGATTCACACAAGGTCATGATTACTCAAGGCCCGAATATTGTCGGAATTCATGCCAACGAAAAAGAGGACAAAGAAACACAAAAGTTTATTAACTGATACCTCAATAAAGAAGAGAGTTGATCTGTGCAAAACAGCGGTTCAACTACAACTAAAAAGCAAACTGCTGCTCAATATTTTGCCGAACAGTCCTCTTACATCACTCCTTTAAAAAATAACTTTAAAGCAGATCAAAGTGCAACAAAAGATAGCACTGTTAACACTAACTATTTCACTAAACAAACATTTGATCTCTTCAAAGAAGTTAATGATGGAAAAGTGCTTGGTTTTAATGATCCAAGTGACTTTAGGAGTGGTAAGTTCCGCAATACTATTGGTTCTACTTTTAACGCAACCATCAGTTCTAAAGTTGACTTTAACAAGTTTTTTGAAAACTTTAAAGCTTCTTTAGGGTCTGGTTTTGAGCGTTAATTTTAAAGAAAATTTTTCTTTTCAATTGCGCTTAATCTAAAGGGCAAAAGTAACAGCAGTTCACTGCTAAGGTTGATTGTGTTAACCCGAAATTTCAATTAATTTCCTTTTCCTCAAAAGGTTTTCCAAAAAAAAAAAAAAAAGTAAAATAGAAAAGCTATTTTCACTTTTGATGCACAAAAAGAAGTTTTTATCAAGAACTTCCTTTGCTTCTTTGTTTTTGCTTTTAGGAACAGTATTGAGTGCTTGTAGCAAAGTAGAGTCCACAAAAAGTGTTCATCCTGTAAAAAGTTTTAAATCTGATCTAAAAAGTCTTCTTAAAGAAACAATTAAACAAGATATTGATTTATCTAAAACAATCAAAACTTCAGAAGGCAAACAACAGATAATAACTTCTTTAAAAAAGTCTTACGAAGTTAATCCGAAAGATACAACAAAATTGTTGTTAGACGCATGAAAACAGAGCTTTGAAGAAGGTGAAATAGGTATTGCGGATCTTGATTTTGAAATAGCTTATCCCAAAACCCAAGATCCTTTTACAATGGAGCGAAAGGTTGATCATTTCCAAATGACATACCAAAGTTTTAAGGATTTATCAATTAAAGCTAAGTTGAGCTATACCTTTAATTGATTTGGAGATTATTCTTCAGGTGGTTTCACTGCTAAGAAAGGTGACAAGCATTATTTCGATCTTTTCCTTAAAATCAAACCTGATCCCAACAAATCATTTAAGGCTGCAAATTTTAAAACTGAAGAAAAAAACTCAACAGCTATTGATGGACAAGAAACTACAAGAAATTTAGAGTGAATTGAGTTTGGTGCTTCAATTAGCTGATCATTAAAAGGCAAAGATGATGCTAGTGAAAAAAGTGTGAAACAATTTTTAGATAGCTATGCTAATAACACATCAGGGTACTCATCAGATATTAATCTCTTTAGTTATTTGGAGTATTTAATTAGGTAATTTGTTGTGATTGCTTGGTTGTACTTGGAAAAATTTTAGCCACTTGCTTTTCGCCTTGAACAGATGGCTTTTTATTTTTATTCAAATTAATTTCATCAAAAACTTTTTTGCAATTTTGTTGACACTTCCAAAAGCTTATTTCAATTTATTTAGTGGAGGAGCAAAATGCAAATCTTTAATTTTTGAAATTGATCAGCAAAGCGTCTGATCCCTGTTGGCAACCGAGTTGCAAGTGTTAGTTATTTTTTAAAAAATCAAAAGAAAGGAGGATTTGACCATTAAAAAAGTAAAGGTTACAGAGAAAGCTTCTGTTGAAAGAATGATAGAAGTATTAAGTGATTCTGAAATTTTATCTTATTTATTAAGTAATAAATACACTGCTGATGGAGTTAATGAAATTTATGTAACGATGAACATTAAAGACAAAGATTCAAACACTCAAAGGAAGCCAGATAAAATGTCTTTAGAGCTTGAATTGTTTTTAAATCAGTTTATTGAATTCTTAAAAGAGCAGAGAAAAAGCTAGCAGTATAAAATACCAGCAGTTAATGCATGGCTGTTGGTATTTTTATTCTTTCACTAAACCCCTCATACGAACTCGTGGACTGAAAGCGGGTGGGTGATACCAAGTTGGTGGCGCTGGTCCGCTCAGCGTTGGTCAGGGTGAAGTTTAATGATGGGACGAGTTCGGATTCTAATAACCAAGATACGAATCAAAATGCCTTGAGTTTTGATACCCAAGAATCACAGAAGGCACTTAATGGCTCGCAGAGTGGATCTTCTGACACTTCCGGGTCTAACTCCCAAGACTTCGCCAGCTACATCCTCATCTTCCAAGCCGCGCCCAGGGCCACGTGGGTGTTTGAACGGAAGATTAAGTTAGAGTTGCCCTACGTTAAAAACGAATCGGGAGCAGGGGACTCCACCACAACTAACAGTGGCTCCCTCTACACCACCCTCCAAGACCTCCTCGTCGAACAACCCGTGACCCCTTACACGCCCAATGCGGGGTTAGCCCGGGTGAATGGGGCTGCTCAGGATACGGTTCATTTTGGTTCGGGTCAAGAATCGAGTTGGAATTCCCAACGTTCCCAAAAAGTCCTTAAAAACAACCCCGGACCCAAAGCCGTCACCGGCTTTAAGCTCGATAAGGGCCGCGCGTACCGGAAGCTAAATGAAGCTTGACCGGTGTATGAACCCCTGGATTCGACCAAGGATGGCAAGGGGAAGGATGAGAGCTCTTGGAACAGTTCGGAAAAAACAACGGCGGAAAGTGATGCCCCGTTGGTGGGATCAACCGGGAGTCAAATGGCTGCAGTCACAGACAGTCAGCAATCAGGTGATAACAATGGATTGGTATCGCTTGCCCAGCGCTCCACTACTGTTGCTGTCCAAAAAAGTGACTCTTCTGGTTCTCAAGGCCAAGGCACAACCGATAACAAATTCCAAAAATACCTCAACACCACCCAGGCCCTGCACCAGATGGGGGTGATAGTGCCATCTTTGGAAACGTGGGCGGGGGAAAACAAGTACTGGAATCGCTACCCGTGCTGCTGGTGGTGTTTCAGTCCAAGCAGCGACCCGGCAATCGTCTTCCACAAATGAAGATCTACCGAATGTAATCACCCAGTTATACCATACTTCAACCGCCCAACTCGCTTACTTAAATGGCCAGATCGTTGTGATGGGTTCGAACGCGGTACCGAGTTTGTGGTATTGGGTCGTGGATGAGCGAACCACGTCCGGAAGGGGTACTTGATGAGCCCACACCGAGTTGAACTGGGGCACCGACAAGCAGAAGGAGTTTGTGGAGAATCAGCTTGGTTTCAATGAAACCAGCGCCACCGACTCGCATAACTTTAAAAAAGCGCTGTTACATCAACCCGCCTACCTCATCAGTGGCCTTGACGTTGTGGCCGACCACCTCGTCTTTGCGGCCTTTCGAGCCGGCGCGGTGGGGTATGATATGACGACTGATACGAACGCTTCGACCTACAACCAAGCGCTCACCTGGTCGACCACGGCCGGGTTGGACAGTGCTGGGGGGTACAAGGCCTTGGTGGAAAACACGGCCGGGCTCAACGGCCCGATTAACGGCTTGTTTACCCTGCTCGACACCTTTGCGTATGTGACCCCGGTGAGTGGGATGAAAGGGGGGAGTAAGAATACGGAGGCAGTGCAAACGACTTATCCGGTCAAGTCCGACCAAAAGGCTTCCGCCAAAATTGCGTCCTTAATTAATGCCAGTCCACTCAACAGTTATGGGGATAAAAAAAGCCATCCACCTAAGGTGAAAGGCAGACAGCTCCAAAATATTTTTGACGATTGAAAACTAACAAAAGCTGTTAGTTTGATGTAGAGGCAAAACTATGAATTTACTAAATTTATTTAAATCAGCGGGAGCAGGCTCGTTTTTTCAACACAAGTCACTCTGCCTGCTCCCACTAAATAAATTGAGAAAGATTTTCAAAGTAGTCAAAAAGTTTGAATATTTTGCAATTTGGGTTTGTAATCCCTTCTAACTATTAATAGGAACTTTTACTTTCAAAAAAGTTTTGAAGTTATTTTGGTGTCTTTACTAACTTTAAGCAATTTAGTTAGTGTAAAGACTCATAATGTTTAAATTAAAAATTAACAACTTCCAATTAGGTTTCAAACTCGTTCAGTGACCAGTGACGAACCACCTCCTTAAACATTTTTACGTTTTTCCAATCAATAATAAAGGAGGTCTTCACCATTAAACGCACAATTTCACTTACTTTGTTTAAGAAAAGACTCAACAAAGATAAGATTAATAATTGTCATGTTTGGGAAGAAGAGTTACCTGATGGTAGCTACGACATGGAATTTAATGGCAATTTAAACCATATGGAAAAACGAAAAAGTGGTTATGTTACCCAAAAGCAGTTTAACGAATATAAAGACTCAAACGACCAACGGCTCATAAAGATTGAAACTACTTTGGCTGCCCAAGGTGAGCAAATTAGTCAGCTAGTTCAAATTGTGTTCCTCCAAGGAAAACAAATTAAAGAACTTCAAGCGGAGCAAAAAGCACAAAGAGTTGAATTTAACGCGCGCATGGATCGCTTTGAAAGCCTTGTGCTTAAGAGTCTGGAATCAATCGGAAACACTTTGACTGATTTTGGTAAACGATTCGATTCAATGGAAACTCGTTTGGACTCTATGGATGGTCGTCTCGATTCAATGGAAACTCGTTTAGACAAAATCGATCCACCTAAATAGTTTTAAGCTAAAAATACCAGCAGCTAAACTGTTTTTGGTATTTTTATTCACAAAAAAGCTAAAACGATTTAACGACCAAAATTGGTTTTAAAAGTAATTTCAAAAAAAAATAATAACACACTGAGTAGAACCATTTAAACATTCAACTTTTAATTAATTAAAAACAGTCAAAATTTGCTTTTAAAAATACTTTTACTTCAGTAACTCAAACTAGTAAATTTCAAGGGTTAAACATCTACAAACTTTGTTAATATTTCTCTAAATTTCGGGTTTCTAGTGTTTATGAAATTTAAGTACGGCGCTATTGTTTTTAGTGGTCTTTTGGGCGTTTCGGCCATTTTAGCTGCCTGTGGGGCAAGGGGCAAATTTAACCAAGTTGATGATGGCAAGATTAAGCTAGCTTCATCTTTAACTTCCAGAAGTGCGTCAACAGCTTTACAAAAAGTTGTTGAAAAGTATAACAAGGTTAAAGGCGTAAATGATTACCCCATTGAAATTACCCAAATTGCTGGCGGTTATGATGGCGGGCGGACTGATCTACAAACCCGAGTTAACGTTAAAGACACGACTAATTTTTATAACCTCATCTTGAACTATCCCGACTTAGTTTCCACTTTGGGACGTGTTGGGATGGAACTACCTTTTGATAAGGTTAAAACTGATAAGTTAGCTGACCGTTTTTTGGATTTCAACAACCGTATTAGTGCCATTTCCAAAAAGGGCATTTATGGCATTCCGGTTTCTTTATCCACCGAAGTTTTATCGATTAATGGACCCGTTTTGCACTATATTTTGAATAGTGCACAAGGAAAGGTTAGTTCTGTCACTTCGGTGCAAAGAACAGCAGATTCTGGTAGTGGAACAACAAATAATAATGGAGTAACAAAACCTTTGAAGATTGATAAAGAAAATGAATCTACAAAGAAACTTTGAGAAGAAATAGAAAACAAGGCAAAAGAAAACGGTAAGAAAACGAGTTCTTCAAGAAGAAAAAGAAATTTAAGTAGTTCTAAACAAATGAGCACTCAAACTCAACCTACTGACAATAGTAACGATGCTAATCAAAGCGACCAGGCGATAGAAAAGACTTGGGGTAAATATCAGGAGGTTGAAGGTGGTTTAAAAGATTACACCTTCAAAGCCAGTGTGTTTGAAAACTGACACGATTTGCTTGACTTTTCTAATAGGGTAGCTAAGTCATTTAGCAATATAAACACTAACACCAATAAAAAGGGCACCGATATTCAAGGTGTTCTGGGAATCGATAGCACTCCTAACTCCTTATTTACCTCTGTTTTTGCTGCAGGTGGCGGTAACTATGACAACTTCTTTTATAAGGTTGCAAACGGTCGGGCTGACTTTAGTAACTTTAAGAATAAAGGCACTTCTTACCAAAACTTACAAAAAGTTTATGGTGATTACAAAGGCTTGATTGACAGCAATGGTCTCTTTGTCAATAAGGGTGGTTCTTATTCATCGAACTTCCAAAAGTTTCACCAATTAGCTTACAGTATTTCCTCGACTTCCGGCTTCTTCTACTCGTTTGCTGGTGAAAACTCAAAGCGCTTGAAGTTTGATGGCGGTAATTTTATTGAGTTTCCTGGATTTACTCAAGCAATTTATGCGCCTGAAAATAATAGTCAAAGTGGTGGAGAGAGTGGCGATCAAACCACAAATAATGAAGCCAATCTTTTAGGCACTTTCAAAATAAAAAGCTCTAATAAATCTAAATCTTCAAGTTCCAAATCAAGCACTAAAGCCGAAACAGGCAAAACTAGTGGTGGAGACCAAAACCAAGGCAAAAAAGGCGAAGGCGCTCAAAATCAAGGCAAAAAAGGCGAAGGCGCTCAAAATCAAGGCAAAAACGGAGGTGAAGAAACAACTATTTACCTTTATAAAAACTCAATTCCATCTGGAAAAAATAAAGGCGAAAATGCAATTCTTATAGATAACAAAACTATTGTGGAACAACTAGAAAGTGCGGCTAAAAAAGAAGATAAGAAGAGTGGCGAATCTACCACAGAACAGACACAAATACAAAGTAAAAGTGTAACAGAACAAAAACAACCCAAAATCATCGGTTACACTACCACTGGCAATGTTCACGAAGACAACAAACACATTTTCCCAATTGACAAACTTACATCTGATCGAAATTTTGACCGCAAAATTATTGTTGGTGCCACTGAAGAAACACTCGATAAATCGAATACTTTGCAGTCCAATGAAGCCATTGTTTTACCAGCACCCTCTAAGTACAAAAGTACTGACACTAACAAAGTAACCATCACCCAAGGTCCGAATATCATTGGAATTCACGCCAACGAAAAGGAAAATGCAGAAACACAAAAGTTTGTGGATTGATTCTTAAACAGTCCGCAAACTTGAGAGGGTAAGGGAAAAGGTAAAGAACAAACTAATAAAACCGCAGCTGAATTCTTTGCTGAATCGGCTTCTTATATCTTGCCACTCAAGGAAATTTTTGAGCAAAAGAATGAAAAGAAAGAAGGCAGCGATCAAAAAGATAGTAAAAGCAATGGTAGAGGTAAACAAACTAACCTCTACACCGAAAAGGCCTTGGAACTATTTAGAGGTATTAGTACCGATCAAATAGTAAGTTACAGTGACCAAGTGACTTTAGGAGTGGTCGTTTCCGTGATGGAATAGGTGCCACTTTTAACGCAGCAGTAAGCTCTAAAGCATCTTTTGAAAAGTTTGTTCAAAACTTTATTGCAACTTTAGGTAGTGATATTTAGCTAATCACTTTTGCAGTCCAGTCAAGGGTTTCATAAAGCTAGCGCTTTATAATCAAATAATGCTTTAAAGCAATTTAGACGACTCCATGAACGACTGACAGTGACTCAAAAACCGGCTGGTTAACGCCAAAACTAAGGCTGTTAGTTTCTGGCTAGCCCAAACTACCAGCACCATCTTAGATATTGCTGAACTAATTAAATGTTGTTCGGATTTAAAGAATACTTCCATTAATGGGTTAGTTGATCTTATAAACCAACAAGAAAAACTCGAATTTAACTTAACAAGGTTAAAGGAAATTGATGGCGAGGATGCACGACAGTTGTTTGGCATTGAAGGTAATGTTTACAAACACTTCCAAACTGAACTTAGTCGCTTTTACAAGCAAACGCGCAAACACTTTCGCGAAACTGGTTCGGAAAGTCTCTTTTTAGGTTTACCAGTAATTGAGGGCATTAACGAGTTTAACGATGTCTTTCGTGCTCCTTTACTCTATGTGGGGGTTAAGTTAAAAGTAGCACCACGACTTGAACGCTTTTGATTGGAAATAAACCGCGAGGAAATCTTTTTAAATCCGACCATCATCGGGGTTGAAATTAACAAGCGTAACAGCTTGTTTAAAAATAACTATGACACCACTAAGGTTGATATTAACCAGGCATTGGAAATCTTTCGTGAGTTGGAATACCAATTTCGGATGCCACTCACCTCGGAGTTAAAAAGTTTTTCCAAAAAGGCTAAGAGTGACTTTAACACTGAAAAACGGACTAACTTCCTCACTAATAACGTTCTTTTAGGAATCTTTGACGTTAAGGGGGATCAACTGTTCCAAAACTTTAACGAAATTCTAAACACTGATCCTGATGTTTTGGATGAATTGTTAAAGGATCGCCGCGATCTCTTATACGACAACCGTGAGTTTCGTGAAAACTTTAACTTAAAGGGCACTTACCTCTTTAGCCACTTAGACATCTTCCAGCAGTACGCTGTCAAACAGGCATTTGATGGGGATGTCATTATTGAAGGTCCACCTGGTACTGGTAAGAGTGAAACGATTGTCAACATCTTGGTCAACCTCGCTTTAAACAAAAAGAAGGTGTTGTTTGTGTCAGAAAAGGTAACTGCCTTAGATGTCGTTTACAACCGGCTAGGTAGCTTTAAGCACATTGCCTTATTTAATGCTTCGGTAGCGAGTGAGAAGAAGCGCTTTTACAGCCAGTTCGCTGATTATGAAAGCTTCTTTACCGATAACTTTTCCAAGAAGGATTTAGTTAACGAAATGCCGGTGTTTGACGGGCAGTGGGTTGATAAGATCCTAAGTGAATTCACTAACCTTCAAAACATTTACGATACCCAAATTAATTCGGGTAACCAGAGCTATTCCTTCAAGGAAATCCTAAGTAGTTTTCCAATACTTGATGTCAGTTACATTAAGATCAAGGAACACGATCGTTTTGATGAATGAGTGCGGGTATTTTCCTCACAAGTTTGATTAGAAAAACACTTAACTTACTTAGCGTTTAAAGCGGAACTGAGCAAACGCTGACAAAACATCGATAACTTCTACGCTTTAAAAGACTTACTGGAAAAAAGAAAGAACATCCGCGTGTTGTGCTATGTGTTGGATTACTTCGAGCAGAACAACAGCATAATCAAACCGAAGCGGGTATTGCTCTATACCCCCACTGAACGCGGGCAAAAGCAGTTGCACCAACTCCAGCAAGACGTTGCTAAGTACAACAGCTTGCAACGTTTTAAATCGGCTGCTAAGTTTGAAACAATTAAGCTCAATTTAGCTAACAAACTAGCACAAAACGCCAAGCCTTTTTTCTTTTCTTGATTCATTCAAACACACGCGCAAACACTGTTGGAAAATCTCGTACAAACCCAAAAGCAATTGGTTAAGGCTAAGCAAAGCTACTTAAGCAAAATTGAGCAGTATGTAGTTAGTTGTAAACGGATTCTCAAAGCTACCATTTTAGCCAACTTCTTTGAGTTGTACCAAACCAATAAGAATGAACTATTGGATATCTGTCGGGAAGCCAAAAACCCTGTTTTAAAGGAAATTACCTGGTGGTTTAAGAAGAACTTTGCACTGTTATCAAAGCTATTCCCCGTCCACATTATGACGTTTGAATCAGCCGCCTTATTAACCCCGAACCAGCGCCGTTTGTATGACTATGTGGTGATTGATGAAGCTAGCCAGGTTTATCTAGAACGTGCCATTCCAATCTTATACCGTGGCGCTAAGTACATTATTGCTGGAGACACCAAACAATTAAAACCATCCAATTTCTTCCAAGCCCGGGCTGAGTATGATGTGGATGAAGAGTTTGAAGATGGTAACGTTGAAGCTGCGGTGCACTCGACTTCGTTACTACACTTTTTGAAGAACCGTTCGCGCATTTTAACGCTGCTCAAATTCCACTACCGCAGTGATTCCGCTAACCTAATTGCCTTTACTAATAACCGCATTTATAACAACGAGCTCATCTTTATGAATAAAGCAACCGCTGACAAGCAGGTCTTTATTGTCCATGATGTCATCGATGGTATTTGACGGAACAACCGTAACCTTCAAGAAGCACGCGATGTCGTACAACGCTTAGAACAGTTGACCCAAACTGCGGAATATCAAAAATCATTAGGGGTGATCTGTTTCAATAAAAATCAGGCCGAGTTAATTGAGTACATGATTGACAAGCAGAACAATCCGCTACTCAATGAGTGACGTGACCGGGTGAACGCTCAGGGCGAGTATGTCGGTTTGTTTGTCAAAAACATTGAAAACGTTCAGGGTGATGAACGGGACATTATTATCTTCTCGTTGGGCTATGACCGTTCTGTCAACAGCTATGGCCCGATTTCCAAGCAAGGTGGGGAGAACCGTTTGAATGTAGCGATTACCCGTGCTAAGCAGCGGATTGAACTGTTCAAGACTAACCGTGCTAGTGATTACAATGGTTTGAGCTCCAATTCCTTGGGTAGTAAGTTATTGGTGGAATACTTACTGTACTGTGAGGCAATGGCCAACAACCAGGGTGAGAGCTTAGACTTCCAAGCAACCCAAAAACAAGCACCCAAGGCCAAGTACGAATTGGAATTGGAGAACCAGTTCTTTAATGAGTTGGAACTTATTTTTGGTGAGCAGTTTACGATTAAACGCAATGTCAATGAGGGTGCATACAGCTTTAGCTTTGTCTTTTACTTTAACGAAAACCCTTACCTAGCGGTTGACTTTAACCCTGCTTTACCCCACTCCAGAAAGGAAGTGAGTGAAAACATTATTTACCGCGAACAATTTTTGAAAAAGCGGAAATGGAACCTCGTTAACATTTGGTTAGACGAGTGAAAACTCAATCCAGGTGGTGTGTTGCAAAAGCTTAGAAATTGTCTAACTCACAGCGAAAATGAGTTTGAAGAAATATAATTAAACGATATATTTCTCGTCATTTAAGAGAAGTGTCGCTAGGCACTTCTTTTTTATTTGCCCACTTTATCACATTATGAACAATCAATCAAATCACTTTACCAATCCTTTGTTACAACTGATTAAGAACGTTGCCGAGACCAAGAATTTAGCCATCGATGATGTTGTTTTGTGTCTTAAAACAGCATTAGCACAAACATACAAGAAGCATTTGAACTATGTCAATGTGGAAGTCAACATTGACTTTAACAAGGGCTTAATGCAAATTGAACAGCTCTTCGATGTTGTAGATGATAACAATGAGGATTATGATGACTTTTTGGAAATGCCTTTAAGTGAGGCTAAGAAGCTCAATCCGAACCTAGAAGTTGGCGGGGTGTTGCGCAAACCGGTTTCACTCAAAGACATTAAGGGCGATCTCATTAGCAAAATGGTATTACTCTTCAACCAAAAGATTAACGAAACAGCTTTTAAAACGGTGATGAGTGACTTCATTAATGAAGTCGGTCAGGTAATTGAAGCTAGAGTAGAGGACATTGATACGAACAAAGATGGTGGATTGAAGGGTTACATTGTTAACTTGGAAACCACCAAGGGTTACATGCCTAAACGTGAGTTGAGTAAGGGCGAAAAGCTAGATATTGGCAAGAAGTACCTCTTTGTGATTAAGGAAATCCAAAAGCAATCTTCGATGTGACCAATTACGCTTTCCAGGAGTGATTCGCGTTTACTGGAATTCTTGCTAAATTCCAACACTCCCGAAATTGCTAACGGTACGATCGAAATTAAAAAGATGGAACGCTCTCCTGGTACCAAGTCTAAGGTGGCCGTGATTTCCAAAGACCCAGTAGTTGATCCGATTGCGGCCATTTTGGGTCCCAAGGGCGAGCGCATTCGCGGCATTAGTGAAGAGTTTAACGGTGAGATCATTGATATTGTCATCTGAAATGAGGACAAGTTAAAGTTCTTGGTCAACGCTGTTTTACCGGCTGAAGTGGTCGGTTACAACATCCTTCAAGACGATGAGCGCGATACCAGCATTGAAATCGTGGTACCCGCCAACCAGATTGCCAACGTCTTTGGTTTTAAAGGAATTAACATTCGCTTAATTAGTAACCTAACGGGTTGGAGTAGTGTGGACGTTTACACCGAAAAAGATGCTGCTGAACAAGGGATTGAATTCACCCGGGTCAACTTTCAACCCCAAGGTATTTTTGGCATCAAAAAGCGCCGCGACAAGATCTCCAACAATCCACGCAACAACAACCAACAGCTCGCATCTGATAAGGTGTTTTACACTTCGAAGGCCAATGTGGTTGATGATGAGATCATAGTCGATCTAGCAAAGCAAGCGGAAGCCAAACGGGTCAAGCAAATCAAGCAGGAAGCAACTAAACCCGAGTTGCAATTACAACAAGAACTTAACCTCGAAGCAACCCCTAAGGTAGCTGCTCCAACCCCAACCCCTGCACCCCAACCAACTCCCGCACCAACTAAAGTTGAGCCAGTACCACCACCAGTAAGCGTTACACCTAAGCCAATCCCCAAGGTCAATAAGCCCAAGCCAGTGGTTAAACCAAAATCAGTCTTTTCGATTACGGTTGAAGCGGATGACAGTAAGACTAAACCAGAAAAGTCAAGCGCTAAAACTAACACCCCACAAACGAAACAAACCTTTGATAACTTTGACGATCTCTAGTCGTTAATCTAACCATTTCACACTATGCAAAGTTGGCGCCAATGTTTAGTTACCAGAAAGTCTTTTGCTAAGCCGCTACTTTTGCGTCTTGTAAAATTAGAGCATCAACTACGGGTGGATTTAGCGCAAACACTACCAGGTCGGGGGTATTATTTAAGTGTAGCTGCTTTAAAACTGGAGCGGAAAAACTTTAAAGCACTGTTACAAAAACGCTTAAAGGTAAGCTGTTCTGACGCAGAGCTAGATCACATTATTGCTTGTTTAAAGGAAAGGGAAAGCGATGTCAAAGCATAAACCACGTCATTTTCAAAAGAATAAGTTTGACAACCGGGCTAAAACGAGTGCCAAACAACAGTTTCGCCAGGTCAAAACCGGTGTTAAAGATGGTGTCTTTGTCTATAAGGGACCACTCACTGTTAGTGAGTTTTGTCTCAAAACCAATATTCCGACGGCCAACATCATCAAGCACTTCTTTTTAAATGGGGTTCCCTTAACCTTAAATTCGGTGTTATCGACCGAACAACTAGCTGACGCTTGTGTTAACTTTGGCTTTGACTTTAAGGTAGAAACCGAAATCACCCACGACAACATTATCTCCAACATTAAGTTTGACGATGATCCCACCCAGTTAAGTCCAAGACCCCCGATCGTTACCATTATGGGTCACGTGGATCACGGGAAAACATCCTTGTTAGACGCCATTCGCCAAACTAATACAGCAGCCAAAGAGTTTGGTGGCATTACCCAAAAGATTGGGGCGTACCAGGTCAAAAACCAGGAGGGCAAAACGATTACCTTCATTGATACCCCCGGTCATGAAGCCTTTACCGGCATGCGCGCCCGTGGTGCTCAAGTGACTGACATTGTTGTCTTAGTGGTAGCGGGTGATGATGGCTTAAAACAACAAACTGAGGAAGCGATTTCCCACGCCAAGAGTGCCAAAACCCCGATCATTGTCTTTATTAACAAGATGGATAAACCAACCGCTAACCCCGACATGGTGATCCAACAGCTCAACAAGTTTGATTTAGTCCCTGAAGAATGGGGTGGTGATACCATCTTTGTCAAGGGCAGTGCCTTAACCAAAGAGGGGATCCAGGAGCTCTTGGACAGTATTCTGTTAGTAGCGGAAGTGGAAGATTACAAGGCCAACTTTAACGCCCACTCATCGGGTTATGCGATTGAGGTGCAAACCACCAAGGGGTTGGGTCCCACCGCTACCATTATTGTTAAACGTGGTACCCTAAAGATTGGTGACATCGTTGTCTTGGGTCCCGCTTGGGGTAAGGTTCGTACCATGCAAGACGAAAACGGAGTCCATCTCCAAGAAGCTATGCCCTCTAAACCAGTACAAATTTCGGGGTTTGACATTGTCCCAGTTGCTGGGGAAAAATTCATTGTCTTTGACGATGAAAAGGATGCGAAGTTAATTGCCAATAAGTTCCGCGAACAGCAAAAACAAAAGCTCAATACCACCCAAATTAACGAGGAGCTCAAACAAAAGATTAAGAGCAAGGAAATTAAGGTTTTAAATCTGATTTTTAAGGTTGACAGTGATGGTAGTTTAGCGGCCATTAAACAAGCTATGCAGAGCATTGATGTGCCGGGGATGAGCGTCAACATTATCCACTCAGGTGTTGGTTTAATCTCGGAAAACGACATTATGTTAGCCAAGGCGTCCGGGGCATTACTGTTTAGCTTGAACCTCGGTTTAAGTCAAGTTGTTAAGAACATTGCTAGTCTTCAAGGAGTGAAGGTCGATGTCCACTACCACATCCCTAAGTTAGCGGAAGAGATCGAAAACATCTTAAAGGGCCAGTTAGAACCAGTGTACGAAGACGTGGAACTGGGGCGTGCTGAAGTGTTACAACTCTGGTACCACTCCAAGGTGGGGCACATTGCTGGTACCTTAGTTAAAACAGGTAAGGTTAAACGGGGCGCCTTGTGTAAGCTGTTACGCCGTAACGAAACCATCTATGAAGGCCGGGTGGACTCATTGAAGAGTGAAAAGAACCCGGTCAACCAGATGGAAGCGGGGAAGAACTGCGGTATTGTTATTAATGGGTGTGAGGACATTCAGGTGTGCGACATTATCTTGGTGTACGAAAAGCAGGAGGTCAAGTCCAAAAGTTAACGATGGCTAGTTACAAAAAGGAACGTCTGGAAAACGACATTATTCGTTTAATTAACCGCACTGTTATTCACGAAATCTACAACGAAACGGTCAAAACCGGTCATGTAACCCACGTGAAACTATCGGATGATTTACTCCATGTAACAGTTTACCTAGACTGTTATAACCGTGAGCAAATTGACCGCGTGGTGGGTGCCTTTAACCAGGCCAAAGGGGTGTTTAGCCGCGTGTTAGCACACAATTTGTACTTAGCCAAAGCCGTGCAAATCCATTTTGTCAAGGACAAAGCAATTGATAACGCAATGCGCATTGAGTCTATCATCAACTCCCTTAAAAAATCAAAGCCTAATTAAAATTAATACTGTTTAATGAAACCACCTAAAAAGCCAGTCAACCCACCGTTTGGTAAGCTAACTGATTCTAAAGCTGGAGTAGTCAAAGCTACCTCTAGTCAAGAGACCAAAAAAGTAGCTGACACCAAACCCAAAAATAAGGGTGGTTTGTTTAGCTTCTTTAAAAAAGACAAAACGGAAAAACCAGCTAAAGCAGCCAAACCCAAAGATGCCTTTAAGAGTGCGATAGCTGAATTGAATCCGAAAGCCAATCCTAAGACAGTCAAAGCGGATGTTGCACCGGCTAAAATTCCCCACAGCGATAAGGGTACGGTTACCCCTGTAGAGTTAAAACCCCAAACGGAAGCACCGTTACCACCAGGAGTTAAACAACCTGATCCTAAAAAAGATAAACCTAAGGGCGGTTTATTTGGCTTCTTCAAAAAAGATAAGAACAAAGATGTCAAAAAGGAGCCTGCTAAGCCAGCTACTCCAGTAAAAACTGAACCAACCTCACCTAAGGTAGAACCAACTAAGGTCAAACCACCGGGCGGTGTACCGACTAAGCCGGTAGTGGAAAAACCAGTGTCAGCTCAACCAACGGTACCATTACAACCAGAACCTACTTTCCCAGTCAAAGCAGCACCACTACCACCAGGTGTAAAAGCAGAACCGGAAAGCAAAAAACGCTTTGGTTTGTTTAAGGCATTCCAGAAAGATGACGCTAAACAACCCAAGCAAAAGCTCAATCTCCAAGACCAACAGGATCGCTTTATTGATGACCCCACCGCCAAAAAGCACTTTAGCGCCTTTAACCAAAAAGTTGGTAACTTACTGAAGGACAAAAAGACCCGTAACCGTGATTGGAAGATTGTGGGTTGAATCCACGGTTTAATCCTTCTGTTCTTCATTCCACTGTTAGCAATCATGAATAAATTTGTGACCCTCCCAGCACAAAGCTATCCTGCTGTATCACTACAAGTATCGATTAACAATGCGTTGTGGGGGATTGCGATCTTTGTGATAGCCAACATTGCTCTACCCTTCATTACGATGTTCGTGCTCTTCTTGACTGGGGTCAGAGATGTGCATGCTAGTCGGCCAGTGCACTATTTCATCTGGGTGTTAATGTTGTTAAACCTTACCTTCTTAGTAATTAGTTGTTGTTTACTGGCTGCTGCTTATGCTAACTTAGACCTTTACAACATCTGACGCAACCTTCAGGCTTTAGACCCGAATAATTAGGGCTTGAGTTCTATGCAGCAAACCTTGATCATCGGTGCTTTTGATGGTTTACACAAGGGTCATCAATTGTTAGCACAAGCGGCTAGTGGTCCAGTGGTGGCCTTGTTAATTGCTAACATTCCCAGTCTACAAAGTGACTGGCTATACGAACCGAAGCAACGGCAAGTGCAGTTACAACAACACTTTCAGTCCGTGGTGCACAGCTATGATGTGATTGAACACAACATTAGTGCCCAAGCGTTTTTCGATCAAATCATTAGCCCACTACGGTGTCAGCAATTAGTCGTGGGTGCGGACTTTTGCTTTGGTAAAGACAACCAGAATGCTGATTTTTTAAGACGTTTATTTCCCAATACCACGATTATTCCCAAAGACAGTCAAACGCTGTCCAGTTCTACCATTCGCCAATGGTTAAAGCAGGGCCAGATAGAACAAGCCAACGCCGTGTTATTGGAACCCTACTTTAGGGAAGGGGTGGTTATTCGTGGTAACCAGCAAGCCCGGTTTTTGGGCTGACCAACCGCTAACATTACCTTAAAGCCGTACATGGTGCCGTTGCGCTGTGGGAGCTATGTGATTACGGTGACTTACCACCAAACCAATTACCCGGGTGTAGGGTTTATCAGTTACAAAAACGACCAGTTAGTCTGTGAAACCCACTTAATTGGTTTTAGTGGTGATCTTTACGGTAAACAACTACGCTTTACCTTCAACCAGTTTATTCGCCCCCAGCAAAAGTTTAGTGGGGTGCAAGCGTTGCAAAAAGCGATTAGTGGTGATTTAAAGCAAGCGCAGAAGTGGTTTGCTCAATCAACAAATTAAATAAAATTTAAACCTAATTTAACTTATGGAAAGTGCCCCCAGTGGTGGCCTGTTGGCCTTAATTATTATCAGTATTATCCTCCTGGCTTGTATTAGTGCCGTGGTTTCGGCTTACGAAACCGCCATTACCTCAATTACATCATACAAGTGGAGCAACTATGTCAAAACGCATAACAAGCAAAAGAAGTTAACGACCAAGATTGTTAACCACTTCCAAAAGAACTATTCGGCTTGTTTAATTACCATCTTAGTGGCCAATAACATTGTCGCCATTTTGGTTTCCAACATCCTCTTTTTGGCTTTAGATCAATCGATTAAGAATCCGGCCATTTCCAGTGCCTTGAACCTCTTAATTAGTGGGGTGTTATTGTTAATGCTGTGTGAAATTACTCCCAAAACCTTAGCACGGATTAACATCATCCGCGTCTTGGTCTACTTTGCGGTGGTAGTGTATTTTTTCTACATCCTTTTTTGACCAATTACCAAGTTAGCCAGCATTATCTTTGCCAAATACGAAAAGGCACCCCCAGTTTCCCGCCGTGATGTTTACTTCTTTATTGATGAAATTGAGCAAAACGGGTTATTTACCAAAGAAGACGGGCAGCTAATTAAACGGACCCTAATCTTTGACCAGGTCTTAGTTGACCAAATCATGATTAAGTGAAACCGGGTGGTGTACTGTTATGAAGGTGATCCAGTCAAGACCATTAAGGAAAAGTTCTTGCACGGCCAGTTTTCCAGAATGCCCGTACTCGACCAGACAAGCAATGAAGTGGTTGGTTTTATCCACCTCAAGGACTTGTTTAGTTCACTCGAAAAGAGCAACGAGCCGTTTGTTTTACAGGAGTTATTGTATCCAGCTGTTTTAGTAAGTAACACCACACCCATCAAGCAAGCTTTGCGCCAAATGCGGTTACACCGGGCCCACTTAGCGGTCGTGCAGGACAAACACCACCACACGATCGGGATTGTTTCTATGGAAGACATCATTGAGGAATTAGTCGGGGAAATTTACGATGAACATGATGAGGTGGAGGCAGTGCAAACGTTGGACAACAACACCTGGTTAGTGTTACCGAACGTAAAAGCCGCCTTTTTCTTTAACAAGTGGATTAAACGGGATTTAGTAAAATCAAAAAACATGACGATCCAACGTTACTTGGCATCGTTTGAAAATGATGGGTTAAACACCCAGAATAAACTAGAAACGCCGTGGTTTATTGCTGAAGCAATTGTCGACAGCGAGAACCCCGAGCAAATTCGGTACGAAATTCGCAAAAAGAGTGATGTTGTGGACTAGAGCCTTAATCTTAGAGTTAAAAACCAATAAGCAAAGCCGCTTGTTGTGGTTACTTGCAATTCCCTTACTAATATCGTTAACCTTACTAACGTACGGTTTAGTGTTGTTTAGTTCCAGTGGTCGAATTGATCATGGTGATCACTTCCACTTACGTGAGCGCTTTGTGTTAACGACCGAAGAGTTAGTTACCTTTGTGGTAGCGAGTGTCGTGTTTGCGCTAACTGTTGCCTTGTTTGGGCTGGGTTGCTGGAAGTTGCTCCAAGGTCCAAAAGTGGATAGAACAAACATAAAGCTAGCAAACAGCAATCCTGCTCCCCAAGCCGTGGTATTGCAAGCTGATTGTGATCACTTTCAGGTAGGGGATCACTGTGTGTTTAGCGCTGAAAAGCAACACTTTAAACAACAGTTCAAACAGGACTTTTTGGGTAAAAGTAAGTTTTCCTTTCGCAATGAACTGTACCGTTTTTGTCTCATTGGTGTGTTAATTTCGCTGAATTTAGCGCTCAGTATGGTGGAAATCCCCGGCATTGTTCTACCGTGGGGCAGTTCAATCCAGTTCCGCTTTTTTAACACCGCCATCTTGTTTATTGCGATTCGCTTTGTTGGGTTACTCTCCACATCGTTAATTGCGATTATTACTCCATGGATTCACCTCCTCCTTCACCCCGTTCATACCCCGATTAGTACCGTTTTTTACATGGGCAATGACTTGGTAGTTCTGTGAATATTCTATTTCTTTTACTACCACATCTTTAAGGCCGAAGTCAAACAAACCACTACGGTTGTCAATAACAAGGAGTTTAGTCAATTAGTCAACACCCACAAAACGAAGGTAGCCAAGGCCTTGGCGTTAATTCCAGTTAATTTAATCTGTGGTTTTATTGAGGGTTTGGGTTTCTATGTCGGTTACTTCTTGATTTTGGGTAAATTTGGTAGTGTAGGGCATAAGATTTTCTATGACAGTCAAGCTAACCGTGACCTAATTAACAGTGCTAACGTGATCTATTTCTTACTCACTACTACCACCATTTTTAGCTTAAAGTACCTGTTTGAACTACTGTTCTTTTACAGTGTCGAGAAGGGTATACTTAACATTTCGCGCCACTTTGGTTTGTATTAAAAGGTAAATAATTTAGCTTTTTGTTTAATCAGCAATTTCCTTTATATATAAAAAAGGTAATACAACGATAGTATAGGGGTTCTAGGGGAGTTAATTTGCAGAAAACAGCTAAACCATTGTTTCACCTGGCAAACAATTTAAAGTTAATGCCATATGAGTGACGTCAAATACTTACAGGTCGAAATTGTCGATAAAAACACCATTCGCCTCCAGGAAGATGGCAAAAAGGGTCAACAAATTAGGTTAGATCAAATTATTAAGGTTGATCAAAGTAACATCTTAAACACTTTGGAGCAGGCACAACGCGAGGCATACGAAAGGGAGGCGCAAAGCCGTTACCAAACTAAGTTGGCCAAAGAACTGAGTGAAAAAGACAACACTTTTTTAAAGCAAAAAGCAGCTTGAAACCAAGCGCACAACACGCAAATCCAACAGTTATACCAACAAATTACTAACTTAGAAAATCAGGTTAACAACATCAAAAGGGAAACTGAAAGTAAAAAGGATAACGAATATCAACAACAAATAGTTAAGTTAGAAACGCAGCTCCAAAGCATTAAAAAGGAGACCGAAAGTCAAAAAGACTTGGAATACGAACGTAAAGCAAATAAAACTAAGGAAGAAAATCAACAAGAATTAGAAAGACAACGCCAGTACTTTTTAGAACAGTTAGAACAAGCCCAAAAGGACATTGAAGAGCTCAAAACGCGTGAAGAGCGTTTTAGTAAGTGAGCAATTGCCAAAAAAGGGAAGGAATTGGAAAAGTGGTGTTGGGAAGCTTACAAAAATTACGAAGAGTTGTTCCAAAACTGTGTGTTTGCCCCTTACAGTGAGCTAGTTAAGGGTGCCAAAAAGAGCATAGGTGTAGAAGACGATGAGAGCAATATTAACGAAAAGGCTGACTTTATCTTCCAGGTATTTAATCCCAATAATGACAAAGAACCCTTCTTTTCAATTTGCTGTGAAATGAAAACAGAGTTTACTGAAAGCAAGAGTCGCACTAAAAACGAAGATCATGTCAAAAAACTGATTGCCGACGCTAAGCGCGCTAAGTGCCAATACGGTTTTTTAGTAAGTGAATTGGAACTCAACACTGAAAACGATGTCCAAGTGCAACGGATGCATACCTCGAACAGTGAAGTGGAGGTTTACCTAGTGCGCCCAATGTTCTTCATTGTGATGTTGCGGTTGTTTTACTTTTTGGCCAAAAAGATGTTTGCCCAAGTTGATGTAAACAGCGAGTACTTAGATAAAGAAGCTTTAAATGCTAGCTTTAGCGATTTAAAGAAAAGCTTACTTGAAAAAACCTTTACTGATCTCAACAAGGTCTTTCAAAACAACATTGATGAACTAGAAAAGATTGAAGGATTAGTAACAAAGTTGAAAGCCGCTAATGAAAAGGCCTTAAATAGTCGTTTAAATAACTGGGAAGAAAAGATCCGTAAGTTTGAATTTAAGCTCAATAAAGACATTGTTAAAAAGCTTGAATAAGGTTCTTTTAAGTAGTCTTTTTAGGGTAGAAATTAGTTTCCAAAAACGGTTTTAAATTAGAGGCTTATTTTTAAAAATTCAAAATAAGCAAATATAATTAAACCGTCTTTTATCTGAATATATGAAACTAAATCTGCGATTTCCTAGCTATTTTTTACCCGTCGTTGCCGCTAGTGCTTTCCTTGTTAGTTGCGCCACACCTAACACTTACGAAGTACAACGCGCTGCCTTGATTCAACTAGTTGAAGAAGACGAACGACAAAACTACATTCAAAAGGGTAGTATGGGTGCAAATGCTGTTATGACTGCTGCAAAAGCAGAAACCAAAACAGCAGAAAAAACAGCAACTTCTACCAAAGCTGCTTCAATAGAACTTAAAAAAACTGATACTGATATTAAGACTACAACTACTACCGAAAACAAATCAGCATCCGGATACAAACTAGATACCCTCTTTGGTGACTACATCTTGTGAGTAGTTGACCACCTTTCTGGTTTACTTTTCTCACCTAAGACTAATAACAGTACAACTACTCAAAAAATCCAACTCATTACCGAAGATAAGATGATTTTAGACGGTGGTAACTTGACCGTGGAAAAAAATCATGAACATGGCCATACTCATAAAAATGGTGAAACTCACGAACATGATCATGATCACCACGAAGGTGAAGAGGAAGTAATCGTTGGTCGTGCCCTTTCTTTTGCTAATGGTCTCTTCTTAGTTGTGGATCTTAAGGAAGAAAAACACGAAGAGAAGAAGGAAGCAAAATCTGAAATGTCAATGAACAGTAAAGATATGGTGATGATGACCAAAACTGAAATGATGTCTAAGGAAATGAAATCCGAACAAAAAATGGAGAAGAAGGAGGAACACGAACATCCTCACAAAAAGCTGAGCCTCAGTACCACGGCTTACAAGTTTGGTCAAAGCTTTAACATCCTGGAATTTACTGGTGCTATGCACCACAAAACGGCACACAATAACGAAACCGAATTTAAAAATTTAGGCAAAAAATACGGTGGTATGACCGAGTACACGATTGTCGACTTTGACTTTAATCCACCAAAACCAACTAAGTAAACGGCTTAACTAGCGCAAACAAGTCCCAATGCTTTTAGCTTGGAGCTTTTTTGCTGTTAGTAAGTTTCTTTTAACAATTAAATGGGTCTTTTAAAGTGACACAATAAGATCGCCAAAAAGCGGGGTTACTACTTTTTTGAAGTTGTTTAATTTCAATGATTATGGGAAAATAATAAGGCCTGCAAGCGGCAAACTAGTTTGGAAAAAGATAAGGAATTAGTCAGTTCAATTGTGACTGCTAAAAGCATGATTGACCGGTTTTTCTGGTCTATTTTGAGCTTTCTGCTCACTAACTTAGTCTTTTTATTTGTGGCTTTTTTAATTCTGATTATTTACTTAATTTCCGAAATCACCCAACAGTTTGCCTTTGCTTTTATCGCAACGATTGTCTTCATCATTTTTTACAACATCCTCTTTTTGAGCTACCTGTTGACGATGTACATTAAAGGCTTAAAACAGATTGAGCAAAAGTCACGTTATTTACTCTTAGTGCTTGATGTTAAGGCTGATGAACTGCTTCCTTTCTCCTTTTTAGGTAGTTTAAGAAAGAGTCACATGCTTGAAGAAATGTTGTTAGAGCAGTAATTATTTGCTACAATCTTAAGGCTTTGGTCGAAGTGGATACACCGAAAAGCGTAGTCATTTTGACCATCAAATTTCACCCTTAAGAACTGAGATATGAACGCCGCTAACGCAGTTAAGTACCCTGAGCTCAAGATTAAGCTCGAGTCCTATGACAGCACCCTTTTAGACCTCACTACGAAAAAGATAGTGGAGGTAGTAAAGGGTGTCGATGTCAAAATTAAAGGTCCATTGCCTCTACCTACCAAAAAAGAGGTAATTACGATCATCCGTTCACCCCATGTGGATAAAGCATCACGCGAACAGTTTGAAAAAAACCGCCACAAACGTTTAATGATCTTGGTGGATGTTAACCAAGGTGCGATTGACAGCTTGAAGCGGATCAAGATCCCCGTTGGAGTAACTTTAAGATTTTCAAAGTAGTTAAAGAGAGAGTATGGAAATTCGTGGTATTTTTGGGGTTAAAGTAGGCATGAGCCAGGTGTTTACAACCAATAATGAGCGCCTACCTATTACCGTTATTTATTGTGAACCTAACCAGGTAGCAGGTGTTAAGACTGAGGCTAAGGACAAGTACAGTGCTACCTTGTTGAGTTTTGACACCGTTGAAAACAAAAAGCTCAACAAGCCTCAACAAGGCTTCTTTGAAAAAAACAACTTAAAGCCAACCAAGCACTTACAGGAAATCCGCAATATGACCGGGTTTGAAATGGGGCAACAAATCACACCGCAAAATCTCTTCCAAGTGGGTGAGTATGTTGATGTAAGTGCCATTAGTAAAGGTCGTGGGTTTACTGGTGCCATTAAGCGCTGGAACTTTAAGATCGGTCCGTTAGGTCATGGGGCTGGTTATCCCCACCGCTTCCAAGGTTCAGTCCAAGCTGGTCGTGGGGGTGCGAGTGCACAAAGAGTGTTTAAGGGCAAAAAGATGTCCGGACACTATGGTCATGAAAAAGTAACGGTACAAAACTTACGCATTGTTGGTTTTGATGAAGCTAACATGTTGGTATTAGTATCAGGGGCGATCGCAGGTCCTGAAGGTGGTGTGGTCTTAATCCGTACCGCTAAGAAAAAACCGGGGGTAGTGAAACCAATTGAGTTAGCTGTTCAAACCGAAAAGGCACCGGAAGCAAAACCGGCCAAGCTTTCCAAAAAGAAACAAGCCAAGGAGTTAGCGAAAGCACAAGCAGCTAACCAGCAAACTGTTGAAGCTAAGGTAGACACTCCAGTAGTAGAACCAAAACCAACTGAAGTTAAAAAAGCAGCACCAGTTGTAGAAAAGAAAGGGGAAGATAAATAATGGCTAAGCTTAAACTAATTAAAATCGACGGTTCCTTTGAAACTGAACCAGTGAAACTAAGCCCTGGTTTAATTGCTAAGGAACTCAAACAACAACCGGTGTTTGATGCCGTGTTAGTAGAACAAGCTTCCTGACGCCAAGGCACCCATTCCATTTTAACCAAGGGCGAAGTCCGTGGTGGTGGTAAGAAGCCTTACAAACAAAAACACACCGGGAAAGCGCGTCAAGGTTCAACCCGTAACCCCCACTTTGTTGGTGGTGGCATTGTGTTTGGTCCCAAGCCCAATCGTAACTACAGCTTAAAACTCAACAAGAAAGCGCACACGGCAGCCTTGCACACTGTTTGGAGTGAAAAGTTAGCTTCAGACAACACCCACCTAGTGGACCAAAACCTCTTTAACAAAACTGAGGGTAAAACCAAGGTGATGATGCAATTTTTAAAGAGTGCCAAGCTCTTGGACAAAAACGTGTTGTTTGTAGTTAATACCCTTAACACCAACTTAGAACAAAGCACCAGCAACATTAAAAACGTGCAGGTCAAACACCTAGACAAGGTTTCTGTAAGGGATCTGATGTTAGCTAACGCTCTTTTAGTTGAAAAAGAAGTTTTAAAAGCTTTGGAGGGCAAATTTAAATAACCATGGATGTAACGAACGTACTGCTAAAACCAGTTCTAACCGAAAAGGTGTACTTCAACCAAATGGGTGAAACCAAGAAGTACGTCTTTGTGGTGAACCCTAAAGCAAGTAAAACACGCGTTAAACTAGCTTTTGAATTAGTGTATGGCATTAAGCCACTGAAGGTTAACACCTTAATTAGAAAACCAACCACCATTCGCGGTGGGAGCCGTTTTCCTGGTTTGAGCAAACTAGAAAAGTTGGCTGTGATTACCTTACCAAAGGGTATTGCCATTTCAGTTACTGGTGAAGCGCCAGAAAAAACCGATAAGCCAGCGGACAAAACCACCCTTAAGGAAAGTACTGTTAAGGAGATTAAGGACACCAAAAACAGTCCAGAAGCTGTTGTGAAAACTGCTGTGGAAGCGTTGCAAATTAAACCAACTGCCGCTCCTGTAACCACCGCCCCTCTACAAACCGTGGCGGTGAAGGTAGCTAAAGAAGTTAAAGAAGTTAAGGTAGAAAAGCCGGTTAAAGTGGAAAAACCAACCAAACCGGCAAAGGTAGCAAAAGAAGCTAAAACGACCAAAGTAGCTAAGGAAACTAAAGCTGAAAAGTCAGTTCAAACAACCAAAGTAGCTAAGGAAACTAAAACTGAAAAGTCAGCTAAAACTACCAAAACTACAGCTACAAAAACTACTAAAACTAAAACCACCAAAAAGGAGGTTAAAAAGTAATGCCTATTAAGAAAATTATTAGTCGTTCTAACAGTGGGATCCACCACTCCACGGTAATTGACTATAAGAAGTTATTAACCACCAATAAGAACAAACCAGAAAAGTCGTTGTTAGTTACCCTTAAAAAACACGGGGGTCGCAACAACCAAGGGAAAATTACGGTGCGCCACCAGGGTGGACGCAACAAGCGCAAGTACCGCATTATTGACTTTAAACGAACGCACTATGACAACATCGAAGCGACCGTTAAGTCGATCGAGTACGATCCGAACCGTTCTTGCTTTGTGTCGTTAATTACTTACGCTAACGGTGCCAAGAGTTACATCATTAGCCCTGATGGCATTAAGGTAGGTGATAAGATCTTGGCTTCCGAACACCCGATTGACATTAAACCGGGCTTTAGTATGCCCTTAGCCTTTATCCCTGAAGGTACCCAAGTGCATAACATTGAACTCCACCCTAAGGGAGGTGGTCAAATTGCCAGGAGTGCGGGGAGTTATGCCCGCATTTTGGGTCAAGACGAAACTGGTAAGTATGTCATTTTGCAACTTTTAAGTGGTGAAACACGCAAGTTCTTAAAGGAGTGCCGTGCTACTGTGGGAGTAGTTTCTAACTTAGACCACAACCTCGTAGTCATTGGTAAGGCTGGTCGCAACCGTCACCGCGGCATCCGTCCAACCGTTCGTGGTTCGGCCATGAACCCCAATGACCACCCCCACGGTGGTGGTGAAGGTCGTAGTCCCGTGGGTCGGGATGCACCCCGGACTCCATGGGGTAAGCGCCACATGGGTGTGAAGACCCGCAACATGAAGAAAGCATCCACTAACTTAATTATTCGTAACCGGAAAGGAGAACAATACTAATGTCACGCAGTGCTAAAAAAGGAGCCTTTGTTGATGCTCACCTCTTAAAGAAAGTGATCGATATGAACAAACAAGAGAAAAAACGTCCGATCAAAACCTGGTCCCGTCGTAGTACCATCTTCCCCGAATTTGTCGGTAATACCTTTGCTGTACATAACGGCAAGACCTTCATTAATGTTTATGTAACTGATGACATGGTGGGGCACAAACTCGGTGAGTTCTCCCCGACCCGTAACTTCAAACAACACACCGCTAACCGTTAGTATGATCGCCTTTGCAAAACAATTCCGTGTGAGAATCTCACCCCAAAAAGCCCGCTTAGTGTGTCAACTCATTGTGGGTAAGAAAACCGCTGATGCCCAAAACATCCTTTCCAATACACCCAAAAAAGCGGCTACCTTAATCGCTAAACTACTCAACAGTGCGATTGCCAACGCTACCAATAACCACGGCATGAATGGGGATGCTCTTTATGTCTTTGAGTGTGTGGCTAACCAAGGTCCGAGTATGAAGCGCACTATTCCCCGCGCTAAGGGCTCGAGTAACATGATTACCAAGCGCTCTTCTAATTTAGTGGTGAAACTATCGGATAACCCGAATGAACGTCAAGAATTAATTAAACAACAAAAGGCCCTAGTTAAAAAACGGGTCGAAGGTCAACAAAAAGCAAAGATGGCACGTCAAAAAGCAGTAACATCAGTGGTAAAAGCGCCAAGTAAAACTCAAGGAGGTGTACAAAAGTAATATGGGACAAAAAGTAAACTCCAACGGCTTGCGCTTTGGTATTAACAAAAACTGGATTTCGCGTTGAACCGCTAACTCCCACGCCCAGACTGCCAAGTGGTTAATTGAAGACGAAAAAATTCGTAACCTCTTCTTTGTCAACTACCGCAATGCCCAAGTTTCGAACGTGGAGATTGAACGTACTCAAGCAACCGTGGACGTATTTGTGTACGCCGCCCAACCCGCTTTTCTCATTGGTAGTGAAAACAAGAACATCCAAAAGATCACCAAGCAAATTAAGCAAATTATTGGTCGTACCACCAATTTAGACCTCACCATTAACGAAATTGGCTCCCCTATGTTATCAGCGCGCATTATTGCGCGGGATCTAGCCAACGCGATTGAAGCACGGGTACCGTTACGAACGGCAATGCGCCAATCCTTAATTAAGGTGTTGAAGGCTGGCGCTAACGGGATTAAGGTGTTGGTATCGGGTCGGTTAAACGGTGCCGAAATTGCGCGCGACAAGATGTACATCGAGGGCAATATGCCACTGTCAACCCTGCGTGCTGACATTGACTATGCCTTGGAAAAAGCGCAAACTACGTACGGTGTCATTGGTGTTAAGGTATGGATTAATCGCGGCATGATTTACACCAAGGGCTTAAACCGCACCCCGGCCCACATTCTCCACCCACAAAAGAAACAACCAAACCGCCAAAACCAACAACCACGCCACTTTAACCAGGGCCAGGTGTTGAGTGCTAATAAGTTAACTGGTAGTGATGTGGAGACCAGCAGTATCCAAGCACTTACTAAGCCTAACAAGGAGGACAAACAATAATGTTACAACCAAAAAGAACAAAGTACCGCAAACCTCACAACGTGAGTTACGAGGGCAAGGCTAAGGGCAACAGCTATGTTGCTTTTGGTGAATATGGTCTAGTAGCTACTAAGGGTAACTGGATCGATGCCCGTGCCATAGAATCAGCCCGGATTGCCATTAGTAAGTGTTTGGGTAAAACCGGTAAGATGTGGATCCGCATCTTCCCCCACATGTCCAAGACTAAAAAACCGTTAGAAGTACGGATGGGTTCTGGTAAGGGTAACCCGGAATTCTGGGTAGCAGTAGTAAAACAGGGCACGGTGATGTTTGAGGTAGCTAACATCCCTGAAAGCCAAATGATTAAGGCTCTAACCCGTGCTGGTCACAAGTTACCCGTGACCTGAAAGATTCTCAAACGCGAGGAGGTAAGTGCATAATGACCGTTGCAAAAGAATTACGCCAAAAGAGTTCCGAAGAGTTGGTAAAACTCGTGATTAAGCTCAAAGGGGAACTGTTAGAGTACCGCTTTAAGTTAGCACACGGGGAGCTAGACAAACCGCACTTAATTAACCAAACCAGGCGGTTATTGGCTACCATTTTAACGATTCTTACCGAGCGTAAGTTGAACTGACAAGAAGAACAAGCTAAGTACAAACTGCTCACTAAAAAAACGAACGAAGCAGCTGTGAATGCTTGGAAGCAACACCTAGAAGCTAACAAAGCGAAGCTGTTAAAGTCACGTGCTAAACGCGAGGATGCTAGCAAGAAATAATGAAACGTAATCAAAGAAAGGTCCTAATTGGGATTGTTAAAAGTACTAAAAACGCCAAGACCGCTACGGTTCAAGTAGAATCACGGTTTAAGCACCCTTTGTACCATAAGTCCGTAGTGCGACACAAGAAGTACCAAGCCCACAATGAGGGTGAAGTACTCGCTAAGGACGGGGACAAGGTACAAATTGTGGAAACGCGACCGTTGTCTGCAACCAAGCGCTTCCGCATTGCCAAAATTATTGAGAGGGCTAAATAGACTATGGTAAGTTTCATGACAAGACTAAATGTTGCCGATAACACGGGCGCCAAGCAAGTGGGCATCATTAAGGTATTAGGTTCCACCCGGAAACGTTATGCCTTTTTGGGTGATGTGGTAGTGGTATCAGTTAAGGATGCTATCCCTAGCGGTATGGTCAAAAAGGGTCAAGTGCTACGGGCAGTAATTGTCCGCACTAAAAAGGGCCAACAACGTAAGGATGGTACCCACCTTAAGTTTGATGACAACGCTTGTGTGTTAATTAAGGAAGACAAGTCACCAAGAGGCACACGGATCTTTGGTCCGGTAGCGCGTGAACTACGGGAACGTGGTTACAACAAAATTTTGAGTTTAGCAGTGGAGGTAGTGTAATGCAACGAATTAAAAAAGGTGATAAAGTAGTCGTAATTACCGGTAAGAATAAGGGTGGTAGTGGCATTGTTTTGAAAATTATGCCCGCGCGCCAACAAGCAATTGTAGAGGGACTCAACAAAGTAACACGCCACAAAAAGAAGGACCAAACCACAAAGCGCGCTGCTAAACAATCGACCGGTAAAGTGCAACAAGAAGCGCCAATCTTCTTGAGCAAGTTAGCTTTATTTGACCAAAAGGCAAAACAACAAACAATTGGCAAAATTAAGTACGTTATGGACCCTAAGACTAATAAGAAGACCCGGGTCTTTAAGAAATCCAACAACACGCTCTAGTTATGAATAATCTCAAAGCACATTACCAAAAAACAATTGCCAAAGAGCTGCAAAAGAGCTTTGCGTTTTCCTCGATTATGCAAGTACCGCGCTTGGAAAAAATTGTGATTAACATGGGGGTGGGTGACGCCATTCGCGACAGTAAGTTCTTGGAATCAGCCTTAAACGAACTCCACCTCATTAGCGGTCAAAAACCAGTTGCTACTAAGGCTAAAAACGCCATTTCCACCTATAAGCTGCGCGCTGGTCAACTAATCGGTTGTAAGGTTACCTTGCGCGGTGAGCGGATGTGGGCCTTTTTGGAAAAACTGATTTATGTTGCCCTACCTCGGGTGCGGGATTTCCGCGGCCTTTCTTTAAAGTCTTTTGATGGTCGGGGTAACTATACCATTGGAATTAAAGAACAAATTATTTTCCCGGAAATTGTCTATGACGACATTAAACGGATCCGCGGGTTTGACGTTACTCTGGTAACTTCAACCAATAAGGACAGCGAAGCATTAGCTTTACTTAGAGCGTTAAACCTCCCACTTGTAAAAGGATAATATGGCCAAAAAATCATTAAAAGTAAAACAAACTAGAATCCCCAAATTTGCCGTACGGGCTTACACCCGCTGTCAGCGTTGTGGTCGAGCCCGGGCAGTTTTAAGCCACTTTGGTGTGTGCCGTTTGTGCTTCCGCGAACTAGCATACGCTGGCGCAATTCCAGGAGTTAAAAAAGCATCATGATAACAACTACAAAACCAATTAAAGCCCACTTTGATCCAGTGGCTGACTTACTAACGAAGATTAACAATGCGCGCAAAGCGAAGTTAATGACGGTAACCACAATTGCTTCCAAGCTCAAGATTGCCATCTTGGAAATTTTGGTCAAGGAAGGTTATTTAGCTAACTTCCAGGTCTTGGAAAACAAGAGCAAGACCAAGCGGATTGTCACCTTTAACTTAAAATACACGCAGCGCCGGATTCCCTCCATTAACGGGGTGAAACAAATTTCCAAGCCGGGTCTGCGGATCTACCGGCCCTTTGAAAAGCTCCCGTTAGTTTTAAACGGTCTTGGGATTGCCATTATTTCCACTAGTGATGGCGTGATGACTGATAAGGTAGCGCGCTTAAAGAAAATTGGAGGGGAGATTCTCGCTTACGTTTGGTAAACAGTTATGTCAAAAATAGGAAACAGAACGATTACACTAGACCCCGCTAAGGTCAACCTTAACTTCCAGAAGGACCACATTGCCGTTAAGGGTCCTTTGGGTCAAATTGAGCTCAAACTACCCCCTAACTTACCGCTCAAGTTTGAACTGAAGGATAATAACCTCCAAATTACGCGGAACAACGAGTTAAAGCAATCCAAGATCTTCCATGGTACTTACAACGCGTTAATTACCAACGCCATTATTGGGGTTACTCAAGGCTTTGAAAAGAAGCTGCGCTTAGTCGGTGTGGGTTACCGTGCTAACGTGGAAGGGGAAACGCTCAACCTCCAGTTGGGTTACTCCCATCCCATTAAGGAAAAGATTCCCAAAGGCTTAACAGTTAAAGTAGAAAAGAACACCGAGATTACCATTTCGGGAATTAGTAAGGAGTTAGTGGGTCAGTTTGCCACTGAGGTGCGCAAGTGACGCAAACCCGAACCGTATAAGGGTAAGGGAGTGTTGTACTTTGATGAAGTGATTGTCCGTAAAGCTGGTAAAACCGCGGAGGGTAAAAAATAGATGAAAACACGAACAGAACAACGGCGCTTACGCCATAAAAGGATTGTGAAGAAGATCCGCGCCACTAACCACGACAACCGCGTTGTGTTAATGGTGATTAAGTCCTTAAACCATATTAGTGTGCAAGCGTGGGACTTTTCCCAGAACATTGTTTTAGCGTCCTCTTCCTCACTTGCTTTAAAATTAAAGAATGGTAACAAGGACAATGCCAAGTTAGTGGGGCAAGACATTGCTGATAAACTAGTGAAGCTCAAACTAACCAACGTTGTCTTTGACACTGGTGGTAGTAAGTACCACGGCCGGATTGCGGCATTGGCCGAAGCAGCCCGCGAAAGGGGGTTAAACTTCTAATTTATGACTGATCAAAACCAAAAAGCAAATCAAGGTAACGGCTTGCAAACCACTAACCTCCAAGCCCACGCACAGCGTAAACACAACCTCCGTCCTTCCTCTGAAGGGATTAAGAAAGCCGTGTCCAAAAAGGAAGGTGGTGGTCACAACCGTAACAACCAAAACCGTCGCTTCCAAAAACCGGCTTTCAAGAGTGAGTTTGAAGAACGCATCGTCAAACTAAAGCGCATTTCTAAAACCACTAAGGGTGGACGGAACATGCGGTTTAGTGTGCTGGTCGTAGTTGGTAACCGCAAGGGCAAGATCGGTTATGGGATTGCTAAGGCCTTGGAAGTACCGAATGCGATTAAGAAAGCCATTAAAGCAGCGCACAACTCACTCCACACAATTGAAATCCACAAGGGTTCAATTTACCACGAAGTAATTGGCCGTAGTGGGGCGAGTCGCGTGTTGCTCAAACCCGCTCCTCAAGGTACGGGAATTATTGCTGGTGGTGCCATTCGGGCCATTATTGAGCTCGCTGGTTACAGTGACATTTACACCAAGAACCTCGGTCGCAACACGCCAATTAACATGATCCACGCCACCATGGATGGGATCTTAAAGCAGCTCTCTCCACGTCGGGTAGCGATCTTACGTAACAAAAACCTCAACGAACTTTAGACAATGGAACTCAATCAACTCAAAAGTGTCCCCAAAGCGCGAAACCACAAGACCAAAACTTTAGGTCGTGGGCACGGCTCGGGTTTAGGTAAAACATCTGGTCGAGGCCAGAAGGGGCAAAAAGCGCGCAAGTCCGGTTTAACCCGTCCTGGTTTTGAAGGAGGACAAACGCCACTGTACCGTCGTTTACCGAAGTTTGGTAACGCACGCAAGGGCTTTTTAAAGCAGGAGTGGGTGGTGTTAAACCTCAATAAAATTGCTAAATTAAAGCTCGACAAAATTAACCGCGCTAGCTTAATTGAAAAGCAAGTAATTAGCGCAAAGAGCCAATTACCAATTAAACTAATTGGCCACACTAAGCTCGAAAAGCCGTTGCACTTTGAGGTGCACAAGGTTTCCAAGCAAGCTTTGAAAGCGGTTGAAAACGCCAACGGTAGTGTGAAGCTGTTAGAGAAATAATGCAAGCCAAACCAACAACTGCAGTTAAACAAAAACAGAATTTTGGGCAACGTTTGTTCACTTTGTTGCGCAACCGTGACTTCATGATCTCGTTTTTAATAACGGTCGTGCTGTTGGTTTTGTTTCGTGTTTTAGCGATTATTCCGCTCCCCGGCATCCAAGTCAACCAAACCGGGCTAGACCAAAACTCAAACGATTTCTTCTCACTGTTTAACCTACTTGGTGGGGGTGGATTGAACCAACTATCGCTGTTTGCGGTGGGAATTAGTCCGTATATTTCGGCGCAAATTGTTATGCAGCTGTTGTCAACGGACTTAATTCCCCCGTTATCTAAACTGGTGAACAGTGGGGAAGTGGGTCGGCGTAAAATCGAGATGATTACGCGGATTATTACGCTACCGTTTGCGTTGGTGCAATCGTTTGCTGTGATCCAAATTGCCACTAATTCCGGTGGCGGTTCTTCACCTATTACCTTAAAAAACAACGGTTCGGATTTTGTCGCGTTTTACATCATTGCCATGACTGCTGGCACCTACCTGTCGGTCTTTTTAGGTGACACCATTTCCAAAAAGGGAATTGGTAACGGGATCACCCTGTTAATTCTTTCGGGGATTTTAGCCCAGCTACCCGAAGGATTTATAGCTGCTTACAGCGTGTTATCAGGTGTAGTGGTAACCATTAACGCAACCTTAACCACTGCCATTAGCTTCTTCATTTACTTCATGGCGTTTTTGGCATTACTGTTTGCCACTACCTTTATTACCCAAGCGACCCGGAAGATTCCGATTCAACAATCCGGTCAGGGTTTAGTAACCGAATCCTCTGCGTTACCTTACCTCCCAATTAAGGTCAACTCCGCCGGGGTAATTCCGGTGATCTTTGCGTCTTCGATTATGTCAATTCCGGTGACGATTGCCCAGTTCCAACCGCAAACCGAATCACGCTGGTTTGTGGAGGACTATCTTTCACTGTCCAAACCAACTGGCATTGTCCTGTATGGTATCTTGGTGATTCTCTTTAGCTTCTTTTACAGCTACATCCAGATCAATCCAGAACGGTTAGCCAAAAACTTCGAGAAGTCCGGGCGTTTTATCCCGGGTATTCGTCCCGGTAAGGATACTGAGAAACACATTGCCAGAGTACTCGTGCGGATTAACTTTATTGGCGCCCCTTTCCTAACGGTGATTGCCATTATTCCTTACATAGTGTCAGCCTTAATCCACTTACCCAACTCCTTAAGTTTAGGGGGTACGGGGATTATTATCATTGTTACCGCTGTGGTTGAGTTTATGTCAGCACTCCGCTCTGCAGCCACCGCTACTAACTACCAACAACTGCGCCGCAACTTGGCGATTGAAGTGCAAAAAACAGCCCAACAAGACAAGGAGGAACAACTGCGCGCTGAAACCCCTGGTATTGGTAACCTATGGTAATGGAAAATAAGTTCTTGTTTTTAGGCGCTCCGGGTGTGGGTAAGGGTACACTAGCTAAGCAAGTAGCCAACACCACCGGATTGTTTCACCTTTCCACTGGTGACATCTTTCGCAGTGTGATGCAAGAGCAGGGTGCGTTGAGTCAAACGCTAGCGCACTATATGAACCAGGGTTTGTATGTCCCTGATGAATTGACTAACCAAACCTTTTGGCACTTTGTCACTACCCACCAAAATGAACTCCACAAGGGCTTTATCCTAGATGGGTATCCCCGTACCTTAAACCAACTCGAGTTTTTGCAAAGCAAGTTGCAATTAGACCAGGTGTTTCACCTTAAGCTAAGTGATCCCCAAGTGCTAGTAGCGCGGATCTTAAACCGTTTGGTTTGTCCTAGTTGTGGTAGTGTATACAACAAGCAGAGCAAACCACCATTGAAGGCCAATCAGTGTGATCGTTGCCATGCTACGTTACAAGCGCGCAATGATGACACGGAAGCGGTAATCTTAAAGCGTTTAACGCTTTACGAAGACACCGTTAAACCCTTAATTGAGGCCTTCACCAAGCAGGGCATTCTCACCGTCATTGAAGCCCAATTACCGCTCGAACAACAGGTCAATTTGGTTCAACAGTTAGTGCATTAATGGTTTATTTAAAGTCTGCGCGTGAGGTCGAACAGATCCGCCAAGCATGTAAGATTTTCCAAGAAGCAAAGGCATATTTTACAATTGAAAGGCTACTTGGCAAATCTTTAACAGCAATTGATCAAGCGTTAAAGCAGTTTATTGAGAGCAAAGGTGCAACTTGTGCCTTTCATAAATACCAAAATTTCCCGGGATTTAACTGCCTTTCGTTAAACGAAACGGTAATCCATGGCATAGCGGACAACCGTGTTTTTGGTGTTAAGGATAAACTGACGTTGGATATTGGCATTAACCTTAACGGTTATATTTGCGATGCAGCTTTTACGGTTTTGGGCCCCAAGGCTCCAGAGCCCATGCAAACGCTGTTAGAGGTAACGGAAGCTTGTTTTACTGCTGTGGTGGAGCCTCAACTGCGCCCCAATAACCCCACTGGTAATGTTTCCCATGCCATCCAAACCTACTTTGAATCTAAGGGTTATTACCTTTTAAAACAGTTTGGTGGTCATGGTTGTGGGATTAAGGTGCACGAAGAACCGCTGATTTTGAACTATGGTAAGCCTGATACAGGTACTAAGTTAGAACCAGGGATGGTCTTGTGCATTGAGCCCATGGTAATGACTGACAGTGATGCAATGGTAATGCATAACAACAGCTGGAATGTATTAACCCCTAAAAGCCGTTACAATTGTCATGTAGAACAGATGTATGTGATCACCACAAGTGGATTTGAATGTTTAACTAATTAATGCAACCAAAGTTTAATAACCAAGCAAAGCAGGACAAACTCGTCTTAACGGGCAAAATCTTGGAAATCATCCACGGTGACAAGTTTCGCGTGTTGTTGGAAAACAACGTTGAAGTAGATGCTCACTTAGCGGGCAAAATGCGAATGCGCCGGTTACGAATTTTACCGGGCGATTTAGTTGAAGTGGAATTTTCCCCTTATGATTTAAAGTTAGGTCGAATAATAGGTAGAAAATAATTAAAATACTATGAAAGTTAGAGCGAGCGTAAAGCCAATTTGCAAAGATTGCAAGATCATTAAGCGCCACCAAATTGTGCGCGTTATTTGCAAAACTCAAAAACATAAACAACGACAAGGATAATGGCACGGATTTTAGGTATTGACATTCCCAACCAGAAACGCATAGAAATTGCGTTAACCTATATTTTTGGTATAGGGCTTTCGCGCTCCCAAGCCATCTTAAAGCAAGCGAACATTAACCCCGATAAACGGGTCAAAGACTTAACCGAAGAGGAGTTTGTTGCCATTAGGAATGTAGCTAGTGCTTACAAGATTGAGGGTGATTTGCGTCGTGAAATTGCACTCAACATTAAGCATTTGAGTGAAATTGGGGCATGAAGGGGCTTGCGCCACCGCAAGAACTTACCAGTGCGCGGCCAACGTACCCGTACCAATGCCCGCACCAGAAAGGGTCCACGTAAGACCGTGGCTAACAAGAAGATTGAAAGCAAGTAATGGCTAAGAAGAAAAAGATTAATGTATCTAGTGGAATTATCCACGTATCCTGTTCCCCAAATAACACAATTGTATCCGCTAGTGATCCGGGGGGCAACGTGTTGTGCTGAGCTAGCAGTGGTACCATGGGCTTTAAGGGATCGCGCAAAAAGACCCCGTACTCAGCCGGCATAGCAGCCGATAAGGTGGCCAAAACCGTCAAGGAAATGGGGATGGCGACCGTTAAGCTCTTTGTCAAGGGTACAGGTCGGGGTAAGGATACCGCCATTCGCAGCTTTGCTAATGCGGGACTTTCTATTACTGAAATTAACGAAAAGACCCCGATTCCCCACAACGGTTGCAAACCACCAAAACGACCACGCTAAGATTTATGGAAAAGTTTTTAAAGTACGAAATAAAGGTAAATAACGAACAAGCACGCGCTAACCCCAACTACGGCATCTTTGAAGTTGGACCACTCGAATCGGGCTTTGGCATTACCATCGGTAATGCTATGCGCCGGGTGCTGTTGAGCTGTATTCCAGGTGCCAGTGTGTTTGCACTCTCAATTTCGGGTGCCAAGCAAGAGTTTGCTGCTGTGGAAGGGATGAAGGAAGATGTCACTGAAGTGGTGTTAAACTTCAAGCAATTGGTAGTGAAGATTTCTGACCTTCTGTTTGAAGATGGTGAAATGGTCGAACCACCACTAGAACGTTGACCATTGTTAACGGTAACAGCTGAGAAAGCGGGACCGGTGTATGCGAAGGACTTGGAGTGCCCAGCTGGCTTTGAAGTGGTCAACAAGGACTTGTACCTGTTCTCGTTGCAAACTGACAAAAAGGTAACAGTTAACGTGTATGTGAAACAAGGACGGGGGTTTGTTACTTTCTTGGAAAACCGTGAAATGATTAATTCTTTGGGGATTATTGCCACTGACTCGAACTTCTCCCCGGTATTACACTGTGGTTATGAAGTACAGGAATTGAAAACTTCCAAGCAAAAGATTACTGATCACTTAACTTTCAAAATTGCTACTAACGGTGCCATATCCGCTGTGGATGCCTTTGCAATGGCTGCAAAGATCTTGATCGAACACCTCAATCCGATTGTTAATGTCAACGAATCGATTAAGGCGTTGAATATTATCCAAGAGAAAGCCGAAGAACGCCGTGTCCGTTCGTTTGCGAAGCAAATTGAAGAGTTGGACTTTACCGTGCGTACCTTTAACTGTTTGAAGCGTAGTGGGATCCACACCTTACAAGAGTTGTTGTCCAAGTCATTAGCTGATATTCGTGAAATCCGTAACCTCGGTAAGAAATCGGAACGCGAAATTATTAAGAAGGTACACGAATTAGGACTCAAATTAAGATCTTAGTTATGTCATACATTAATAAACCAGGTAAAACTAGTGCGTGAAGAGTCATGACCGTGCGCCAACAGGTAAGCGCTGTTTTGGCTTATGGCAAGATTGAAACAACCTTAAAGAAGGCCAAGAACACCCAAAAGCGTTTAGATAAGTTAATTACCTTAGCTAAAGTTGATAACTTTAATAACCGTCGTCAAGTCAAAAAGTGGTTGTTAAACACCAACTTGTTCGATGTTGATCAACTAATGGATCACTTGTTTAGCAAAGTTGCCCCTAAATACGAAAAAACCCCCGGTGGTTACAGCCGTGTTTTAAAATTAGGGCCACGTCGCGGAGATGCCACGGAAATGGCCATCTTGCAACTAACTGATGCTAAATACAAATAAACAAGCAGCTGCAAGCTTTCAAGGTGTTTATTTTTCTTACAGCGAATTACCATTAATTCGCAACCTCTCCTTTGATGTTTTCGAAGGGGAGTATGTTTGCATAGTTGGCCATAACGGCTCGGGTAAATCCACCATCTCGAAGCTGTTAACGGGCTTGTTAAAACCACAAGCGGGTACCATCAAAATCTTTGGTAAGACGATTTCGAGTGAAAACGTGACTTACCTACGCAACCACATTGGCATTATCTTCCAAAACCCCGATAACCAGTTTATTGGTATTACCGTGGAAGATGACATTGCTTTTGGACTGGAAAACCGCCGTTATACGCGCGAAAAGATGAAGAGCATCATTGAGGATGTAGCCCAACAAGCGGGAATAACGGAGTTATTGCAAAAAGAACCGCATAACCTTTCGGGTGGGCAAAAGCAACGCGTTGCCATTGCCTCGGTGTTAGCCTTAAACCCAGCCATCATTATCTTTGATGAGTCGACTTCGATGTTGGATCCTAAAGCGAAGCGTACCATTAAGCAGTTCATGGTGGAACTGCGCAACCAGGGCAAGTGTGTTATCTCCATTACCCATGACATGGAAGAGGTTACCAAAGCAGACAAGGTTTTGGTCATGAATGAAGGTCGTTTAATTCGTCAAGGTAAACCCAAGGATGTCTTTAACAGTGCAGCCGAATTGCAAAAAATCCGCTTGGATATTCCCTTTTCTTTAAGCCTTTCCACGAAGGTTAATGGTGTGACTAGTACAATAGACTACCAACAACTAATTGAGGCCATTGGTAAGCTATGAAAAAAATAGTTGAACTCAAAAACCCCTTAAGGGATGGAGAAATCCTCAGTGTTAGTAACCTTTCGTGCTTTTTTAACGAAAAAACAGTCCATGAAGTCAAGGTAATTGACAACTTTAGCTATACCTTTGCTAAAAATAAGGTCTACTGCATTATTGGTGACAGTGGTAGTGGTAAATCAACCTTAGTCAACCATTTTAATGGTTTAATAAAGCCCGCTTACGGAGATATTTGGGTCAAGGATATTTACATTGGTCAAAAGCAACGCAAAATTAAGGACTTTAAACGCTTGCGCAAAACAATTTCGATTGTCTTTCAGTTTCCCGAATACCAACTGTTTAAGGACACAGTGGAAAAGGACATTATGTTCGGTCCAATTGCTTTGGGTCAATCGAAAAACGAAGCACGCCAAAAGGCAGCTTACTACCTCGAAAAAATGGGGTTAAAGTACACTTTTTTAGAGCGCAATCCGTTTGAGTTGAGCGGGGGGCAAAAGCGCCGTGTAGCGATTGCTGGGATTTTAGCAATTGAACCCGAAGTTTTAATCTTTGATGAACCTACCGCTGGACTAGATCCTGAAGGGGAACGAGAAATGATGCGTTTAATTAAGGATGCTAAAGCTAGTGGTCGCACCGTCTTTATGATTACCCACCAAATGGAAAATGTTTTGGAAGTAGCGGATGAAGTGCTGGTGTTGTCCAAGGGCCAACTCGTTAAATCCGGTGATCCTTACGAAGTTTTTATGGATGAAGCATTTTTGGCACACACCACCATTATTATGCCTCCAGTCATTCAAGTTATCAAAGACCTAATTAACTTAAACCCGAAGTTTAGTTGTTTATTAGACTTTAAACCGCGCAACTTAGACCAGTTGGCGGATGCCATTAACAACACAATAGCTCATGGATAGTTTTTTTATTAATGGTTATGTACCACGAGATACCTTTATCCACCGGCTCCACCCGACAACGAAACTGTTAATCTTTTTGCTGTTCGTGATCCTGGTGTTTGTACCCATTGGCTTTGTTTTTCAAAGTGTGATCTTTGTCTTTGCCACAGTTATTTTCTTTGTAGCCAAACTACCCGGACGGTTTTACCTCTCCTCGATTAAGTCAATCTCACTGTTATTCTTACTACTGTTATTTGTCAACTGGTTTACCTTCCGTGACCCCGGGTTCTACATTACCGCTGATCAGGTTAATACAGTAAAACCACACTTTAACGGTAATAACTTTAACTTCTGAAACATCTCCCTGTTTAACTATCAGGACAATGTGTTTTCGCAAGTGTTTAACTTTAACCGTGCCAACATGACAGAGTTAAACAAGATTAACTTCTTCTTTAAGGAAACAGCTAATGCTAATGCATACACCAAGGTAACGGGGATTGATAAATTGGCAGAAATGTTGGCTTCAAAGAATCTCTTTAAGTTCAACGGTTCTACCAATGGCATTGATAAAAACAAGATTCTTGGTGCCTTTCTAGATCATAAGATTGCCGTTTACCTCGGTCGTAGTTGGGGTGGTGACTTTAGTGGTTTTGTGATTGACGTAAGTGTAAGTGATAAAACATCTACCTTCACGATTAAACCCTTTTTAGCTAACTCGAACTATGTGTTGACACTGCGTGCCATTATTCTAGCCTTTTACGTTACCCAAAAGATCTTGATCATGATCATTTTGGCAACAGTGTTAACTTCCACTTCAAGCTCGGTTGAGTTGGCATACGGAATTGAACGCTTGCTGTGACCGTTGAAGCTGTTACGGGTTCCAGTCAATGTGTTTGCGATGACAATTGCAATTGCAATTCGCTTTGTCCCTTCTTTATTGTTGGAATCACAACGGATTTTAAATGCCCAAGCATCACGCGGTTTGGACTTTAAGAACGGCAATTTCTTTGTCAAAATGCGTTCATTGTCTTCCTTGGTTGTACCGATGATTTCGATTGCATTCCGCAATGCTGGGGAGTTGGCGAGTGCCATGGAAGCACGTGGTTATGATCCAACTAAGAAACGTACTACCTACCGCAAGTTTAAGATTGATTGGGTTGATGCCACAGCTTTAATTCTCACCGCTCTTTACTTTGTGGTGATTATTTTCTTAACCGTTAAGGGTGCAGTGTTCCTAGATTTAGGGACACCTGAGTGGTTGCTAACCGGCAAGATTAAAGAGCAGGTAGAGCGCTCATTATCAGTTAAAAGTGCCTAGGTACTTAGCGATTGTCGCTTACAATGGTGCTCCCTTTCGTGGTTGAGCCAAACAACCGGGTTACCACACCGTTCAGGGTCAAATAGAACAAAACCTAGCCTTAATTTACCAAACACCAATTAATATTTATGGTTCGGGTCGGACTGATAAGGGAGTGCATGCCATTAACCAAACCTTTCACGTTGATCTACCTGATAAGTTACCACTCAAACAGTTAATTGCCAAACTAAACCAATTAAACTACCCTGACATTTTTGTAAAGGCCTTAGTTCCAGTAGCACCGCGATTTCACGCGCGGTTTGCAGTAAAAACTAAGGTTTATGAATACCTTATTAACACCCGAGCTTTTAACCCAGCACAGTACCACACCGTGTGACAGTACAACCACCCATTGGATTTAGCACAACTTGAAGCGGATACCACGCTCTTTTTAGGCAGGAAGGATTTTTGGAGTTTTAGCACCGCTAGCCAAAAGGACACGCAGCGCACAATTAGTAAAATTATCGTGCAACAAGATCACACTGGTACGGTTAAACTGACCTTTTTTGGCAACGGTTTTTTAAGAAACCAGATTCGAATGATGGTAGCTTCTCTAGTGGCCTTAAACAATCGAACCCTGATGCGAGAAACAGTTCAACAGCTGTTTGATCAACCACAAAAGGGTGGGTGTAAGGTTAAAGCCCCCGCTTGTGGTCTTTATTTAAAAACTGTCTTATATGAATAACCAATACAACTGAAACCTCGAAGTTTTACTAAATGGTAAGAGTTTAGCTGATAACTTTACCGAACTCAAACAACTCAGTGAGCAGGAAAAAGCACTTTACGATGGTGGTGCTTGCTTTCAAACCAAAGCTAAGTTCACCGAATTTTTACAACTACAAGAAAAGATTGAGGTCTTAGAAAACCGTTACAGCAATTTTTTGAGTAACAAGCACGCTGAAAACAGTTTGGACAAAACGATTAACGACGCTTTATTTCAGTACGAAATGTTTAAAAGTGAACATGCCTTGGTGTTTGTTGACTTTGAAAAGAACCTGTTTAAGCACGAAAAGGTGATTCGCGCATACCTCCAAGACCCTGCTTTAAAACAATACCAAAGGGATTTTGAACTAGTATGACGGAACAAGAAACACCAAATTGATCCCGCTAGTCAAAAGTTGTTAGCGCAAATTAGTCCGGCATGAAACCAAGCGGACAAGATCTTTAATGTGTTATCTACCGCTGACCTAAACCTCCAACCAGTCGTATACAAGGGTAAAACTTACGTTATTAACGCGGTTAGTGATTATCAAAGTCTCTTGGAAAATAAGGATCGTGGCCTGCGTGAAGCTGCTTACAAAGTGTGGTTGGAAATTTACTGACCCACACGCAATACCTTGAGTGTGAGTTTAGTGGAAAACTACATCCAATTAGAAACCTTTGCTAAGTTAAAGAAACACCCCAATTACATTGCCAAAACAGCTTTTGATGATGAAATTGATGTGGCTTTCATTGACTTTGTTTATGAGCAAGTGGCTAGCTTTGCCCCGACCTTTAAAGCCTTTCAAAGTTTAAGGAAGCAAATTTACAAGCACGTTTTAAAACTAGACAAAGCCCAACCCTGGGACTTGTCGGTACCGTTGTTTAAGGCTAGTGGTGACTACACCATTGAACAAGCCCAAACTGATGCGCTTAAGATATTAGCACCTATGGGTAGTGAATACCTGGAAGTGGTAAAGGAAGCCTTTCGGGAGCGCTGAATTTCCTGGTTACCCGATAAGAACAAGTATACCGGGGCTTACTCAATTTCCAACGTTAAGGGTTTAGATCATTACTTCATTTTGATGAATTTTGATAAAACCCGCGCTTCATTAAATACCCTAGTCCATGAATTGGGTCACAGTGTCCATTCTTGGTATGCATCTAAGTACCAAACGCAAAACTTGGATCCTACAATTTTCTATGCTGAAATTGCTTCCATTTGTAACGAATTACTGTTGTGTTACCACGACATTATTAACTATGAAAACCGCAATCCGCAGCAGTTAATCCGTAGTTTAATGGAACAAATTAGCCACTTCTTTGGCGCTACCACGAGACAGTTAATGTTCTCTCAGTTTGAACAAGACACCTTAAAGTTAATTCAGCAAAACCAAAAGCCTGACTTTAAGACCCTAGTGGAAATTTATGGTAAGACCGCCATTAAGTACCAAGCTGCTAATGCTGACGCTATTACGAAAAAGCTCAAGCAAACCAAGTACCAAAAGTCATTGGCTTACATTACCTCCATTCCGCATTTTTACGCTGGTAACTTCTATGTTTACAAGTACGCCATTGGTCAAGTTGTGGGTACCTTAGTTGGTAAAAAACTGAGTGCTGGTGACAGTAATATGCTCGCTGCTTACAAGCGCTTCTTGTCTTCAGGCTCCACCTTACCACCACTAGAAACAATTAAATTGTTAGGGATTGACCTTACCCAACCAGAACCGTGACAAGAAGCCCATGCGGAGTTAAAGCGCTGGATTAAACTAGTACAAACAGCCTTTAAACAGCTGCAACACAAAAAGCGCTAGTGCATTACTTTAACCGCGCCAAAAAGGCAAAGAACAATGAGTTTTATACCTTGTTTGAAGACATTGCCGCTGAAGTGGCATGTTATCCCAATGCCTTTAAGGGTAAGGTAGTGTTGTGTAATTGTAATGATGGTTACCAATCGAACTTCTGGCAGTTTTTTCAAAGTCAGTTTCATGCTTTAGGACTCAAAAAACTAGTTGCGATTGCTTTTAATCCACTTGGCAACAGTTATCAACTCAACTTCGATGGAAAGGAAATAAAGGAACTACCTTTAGCTGGTAATGGCAGCTTTGACAGTGCGGAAGCCATTGTTCTTTTAAAGCAAAGTGACATTGTGGTAACTAATCCACCGTTTAGCTTGTTTCAGGACTTTGTTTGTCTTTTAGCAGAGCATGGCAAACAGTTTTTGGTTTTAGGTCACAATGGTGCTGTCGGTTATAATCAGATCTTTAAGCTGTTTAAGGAAGAACAGTTGTGATATGGTCATACTGTTAACAGTTCGATGTTGTTCCAAGTGCAGTCTAACTTTAAACTGTATGACCCCAAAAGCGTTAACTTTGTTAAAAAGGATGGTCAACTGTTTCAAAAAGTACCGGGAATTTCCTGGTTTACAAACCTCAAGAAAAACCAACAACCAGCTTGACTAAAAACAAAATCACGCTATCAGGGCAACGAACACAAATACCCCAAGTTTGACTGGTATGATGCCATTTTTGTCAGTAAGGTTAAAGAAATTCCCCTAGACTGATTTGGTTACATGGGGGTGCCATTAACCTTTCTCAATTGCTTTAATCCCAAACAGTTTGAGCTAATTGATTGTCTAGCCAATCCCTACGCTACCCTAGACACATTAAAAACTAATGCTTATGTGAGATCACACCACGGTGATGTGAGGAATGTGAAGGGTAAGCGAAGGTATGTTAGGGTAGTGATTAAACAACGTCAAAATGTGATTTAATGCCTTAAGTTAAGTAATTTTTGCAAAAAATTTCCTTTAATTTGCTAAATTATTTTAAGGATAGCACACTGCTGTCAAACCAAATGAGAACGAATGAAATTCAAACTATTTTTGGGCAGCAGCTTTTTTGGTGTTGCCACCTTACTTATTGCTTGTGGTACTGGTCGCTTTGATCAAATCGATGATGGCAAAATTAAGCTTGCTGTTGTCACATCCCCTAGTGCCGCTAGTTCACTGCAAACCTTATTAGATCAATACAACAACACTAAAGCACCAGCTGATTATCCAGTGGAAGTAGTTTCACTAGACAGTACTGGGAGCTATACTAAAGGGAAATTTGATACCCAAAAACGGTTAGCTGCTAAAGATAAAAATAACTTTTATAACCTAACCTTTAACTACCCCGACTTAGTTTCATCTTTAGCAATTAACGGAATGGAACTCAACCTTGATGGGGTTAATGTTTCTAACTTCGAACAGTCTTTCTTAGACTTCAACAAGAACATTAGTGGCGTACGCAAACCCGGCATTTACGCCTTACCAGCTACCATGAGTGGTGAAGTTTTGGTTTTAAATGGGCCAGTTCTCCACTACATCTTGAACAGTGCCAAGAAGAAGGATGGTACCGAATCAAAAATTAAAACTGCTCAATTAAAAACAGCTTCAACTCAGGCAGAAGTTAAGGGTACTATGACCATGGCTAGTGATGAACAAACCACAAAGCTTTGGACTAATATTCAAAACGCCGCTAAGGAAAATGCCGCTAACGGTACAACTGAGAAAGCGGAAAAAACAGTTTCCGCTAGTTCGTTGCAATTAAAAAATACGAATAAAACCACCGAAGGTACCTTAAAAATTGGTACTGATGACAACACTAAAAATCTTTGAAAAAAGATTGAAGATGCCGCAAAAACTAACGGGGAAAAAGGTAATGAGAAGCAAGAAGCTACTCAAAGCAATGCTAGCGCTAGTCTCGTTAAGTTGGATCAAAAAAATACTAGTCAGGATAAAACTCAAAACACCCAGACTAGTGATGATGAAATTAAAAAATCTTGAGGCGAATATAAGGAAGTAGAAGGCGGTCTTAAGGGTTATGAATTTAAGGCTGATGTCTTTGAGAGTTGGGAAAAGTTAAACGATTTTGCAGTACGGGTAGCAAAGTCGTTTAGCAAGGTTTCTGAAGAGAAGAAAAGTGGTAGTGACATCCAGGGTGTTTTTGGCATTGGTAGTTTAGAAAATGCGCTTTACACTGCATCATTTGCAGCCGGTGGTGGCGACTATAACAACTTCTTGTTTAACATTAAAAAAGGTCGCGCTGATTTTAGTAACTTCTTTAACCACAATTCAAAAACTTTCCAAAGTCTAAGAGATATTTTCAACAGCTTTAAGCCTTTAATTGACCAAAAGGGTTTAATTAGTAACAAACACTTTGATACTCCAGTGAATAATTACGCTAAATTTCACCAATTGGCATTCTATGTATCATCGACAGCTCGCTTTCCTTACTCGTTTGCCAAAGATAATGTAAAACGCTTAATTATTGGTAAACGCGAATTGGAAGTAAACCCTAAATCAATGTTTGCCATTAAGAAGGAAAACGGCAATAACGGTAACAGCAACCTCTTAGGTAAAGTTGCACTAGATAACAATAAATCTATTGAACTTTACGAAAATAATATTCCTAACGGTAAAACTGATGCCATCTTAATTAAGAACCAAACTTTAATTAGTGCGTTGAAGAATCCAAAACAGACAAAGAGCTCTCAAAGATCTTCTCAGTCTACAAACACCCAAAATGATGCCATTTGTTACTTAGCCTTCAACAGCAAGATTAGACCAGACGATAAAGACATTTTCCTATTAGACAAATTTGGTGAAAAATTTGTCACCGCAATTGTTAACTTTGAAGAAAAAACGGAGGTCAAAATTAATACGCTCCAAGAAAAGGAAGCTGTTGTTTTACCAGCACCACAAAAGTTTAAACCTACTGATCCAAAATCAGTGGCACTTGTTCAAGGTCCAAGTTTAATCGGTATTCACGCTAACGCTAACGAAGACCGTTCAACCATTAAGTTTCTCAACTGGTACCTTAATGCTGAAGTTGATTGAAAAGATGGAGAAAAGAAAACACCAGCAGAATACTTAGCAGAAAAAGCTTCTTACCTATTGCCGTTTAAAAAAGTATTAGAAAAATCCAAGCAAAACATAAAAGCGACCTCTAAGGAAGGAGAGCAAAATCAAGGCAAGAAAGGTGATGGTGCACAGAACCAAGGTAAGAAGGGCGATGGAGCTCAAAATGGTAAGAATGATAAAGCAAAGCACAATGGTTTTGTCGATATTGCCGTCAGCCAATTTTTAAACGGTTCTGTCGCTAAGTTTGTGGAACCAGCTGACTTTTTAGGTATTCGCTTACGTGATGCCGTTAAGAGTACCTTTAACGCTGCCATTAACAACACCTCAATTAACTTTGACAAGTTCATTCAGTACATTAACGATTCTTTAGGTAGCGACTTTAAGCAAAGACAGTAGTTTAAACTGATTCTTTACCTCTAAATAAAAATACTAAAGCCCATTAAAGCCGAACCACTAATTTTGCCCTAGCTTGTAAAGCAGTTAAATATTTTTTAAGATATCTTCACCTCTTTTTTGCGTTTAGTGATGAAATTTAAGTATGGTGCCATTGTTTTCAGTGGTCTTTTAGGAGTCTCTGCCATTTTAGCTGCTTGTGGTACACGCGGTAAATTTGACCAAATTGATGATGGCAAAATTAAGCTAGCATCTTCGTTAACTTCCAAAGGTGCCGCTAATGCTTTACAAGCAATTGTGAAGAAGTATAACGAGGTTAAAAAGCCTGGTGATTACCCAATTGAAATAACCCAAATTGCGGGTGGTTACGACCAAGCACGCGTTGACTTACAATCACGGGTTGGTGTTAAGGATAAAACCAATTTTTACAACCTCATTTTGAACTATCCCGATGTAGTTTCAGTGCTAGCACGTAACCAAATGGAACTGCCATTTGATGGTGTTGATGTTTCTAAAATTTCACCTAACTTTTTAAATTTTAACGAACGTATTAGCGGAGTTTCTAAAAAGGCTAACTATGCCATTCCGGTCTCTGTTTCGACTGATATTTTAGTGCTCAATGGACCAGTTTTGCACTACATTTTAAACAGTGCTAAAGGTGAAAGTAAGGGCGCACAAAAAGATAACAAGAGTGCTGAAGTTCAAAGAAAAAGTACAGGTCAAAAGACCGTTACCCAACCTTTAACTATTGCAACTGATTCTGCTACAAACGGTCTTTGGAAAAAGATTGAAGATGCTGCAAAAGTCAACGGTAAAAAGAAAGAAGAGAAAAAGAGTACACGCAGTAAAAGAGCCACAGAAGGCACACAAACCACCAAAGAAAATACGGGTGGTGATGCTGCAACAAGTGATACCAAAATTAAAGAATCTTGAGGTGCTTACCAAGAAGTAGAAGGCGGCTTAAAGGGTTATCAATTTAAAGCCATTGTTTTTGAAAACTGACACGATCTAATTGATTTTTCTACCAGAGTAGCTAAATCATTTAGCAAGGTCAAAGATAACAGCAACAAGAAAGGCAATGAAATTCAAGGTGTGTTAGGTGTTGATAATTCACCTAATGCTTTACTATCTTCGGTGTTTGCTGCTGGTAACAGTGACTATAACAACTTCTTTTATAAGGTGCAAAATGGTCGGGCTGACTTTAGCAACTTTAACAATAAAGGCAGTTCTTATAAAAACCTTAAAAATGTCTTTAACGACTATAAGAACCTAATTGCACAAAATGGTCTTTATGTAAATAAAGGCGGCTCTTACTCTTCAAACTTCCAAAAGTTTCACCAGTTAGCTTACAGCATTTCCTCTACTTCTGGTTTTGCTTATTCGTTTGCTGGACAAAATTCCAAACGTTTTAAATTTACTGATGATGGTACTTTTGTTGAGTATCCTAGTTATACAACTGAAGTTAATGCACCAGAAAGTAATAATGGCAATGATGGAAAGCAACAAGGTCAAAGTGATCAAGGAAACCTTTTAGGAACTTTTGAAGTTGTAGATAAAAGTACTAGTGATATAAAGGTGAAACCTAAAACTCAAGCGGAATCAAAGAAAAGTAGTGATAGTAAACAAACTGCAAACACAGGCAAAGGCAGTAATTCAAAACAACAAACTCCGAAGAAAACTATTTCGCTTTATAAAACCAAAATTCCTCAAGATAAAACAGAAAACGTTGACGCTTTTTTAGTTACTGATTCAGAATTAATTAGCAAACTTGAAAAAGCTAAAAATAAAAAGGAAGAAACTAAAGCTTCAGGTAAATCTGCATCTGCACGTGTGGCAGTTCAAGCAACTCAAAAGAAACAGTCTAACGAAAAACAGATAGTTGGTTACACTACTACATCCGCATTAAGCGAAGACGGCAAACACATTTTTAAATTGGGTAAACTCAACAGCGAGAACTATGAGCGCAAAATCATTGTTGGTGCTACTGTAGAAACACTAGAACAATCCACTACCTTGCAAAGTGAAGAAGCAATTGTTTTAGCAGCACCTGGTAAGTACAAGGATAGTGATCAAAAGAGAGTTATGATCACCCAAGGTCCTAACCTAATAGGGGTTCATGCTAATACTAAAGAAAATGAAGAGACTAAGAAATTTGTTAACTGGTTTTTAAATAAAACCGAAAGTTGAGAAGTTAAAGGAAACGGAAAGGATTCACAAACTACTAAATCACTTACACCAGCGCAATATTTTGCTGAATCAGCATCTTATATTTTGCCGCTAAAGGAAACTGTTGAAAAAGATCATAAAGAACAAACTAATAAAAACACCTATGTTGCCAAAGCTTTAGAAATGCTCAAGGAAGTTAGTGAAAACAAATCAGTCAGCTATAGTGATCCGAGTGATTTTAGAAGTGGTAGATTCCGTGATGCACTTGGTGCTAACTTTAATGCCACTATTAATTCAAAAGCAAATTTTGAAAAGTTTTTCCAAGGTTTTAAAGCTGCATTAGGCTCTGATTTTGATAAATAGAATAGAAGTTTTTAGTTGCTTTTTAGATAAAAATTTCAAATATTTCTCCTTTATAAAACAATCTTTCAAAAAGCTTTTTAGAAAGAAATTAAACTGTTACTGTCGTGGAGATTAAAACTTACAAAATCAAAGATATTTGTGATATTACACGCGGTAGAGTTATCAGCAAATTGGACATTAAAAAAGATCCAGGAGTTTTTCCTGTTTATTCGGCAGCTACTAATAACGATGGGGAGTTTGGTCGAATAAATTCTTATGACTTTGATGGTGAATATGTTACTTGAACAGCTGACGGTTATGGAGGGGCTGTTTTTTATCGTAATGGTAAATTTAGCATCACAAATCTTTGTGGTCTTTTGAAAGTAAAAAATAAAGAAATTAGTTCAAAATATCTTGCTCATATTTTGAAACTAGAAGCACCGAAATTCACAAACCGTGTTTTTAAAAATAGACCTAAGTTAACACATAAAACAATGGCCGAAATTCCCATTGATTTCCCACCCTTGAAAATTCAAGAGAAAATTGCTACTATCTTAGATACTTTTACCGAGCTAAGGGCGCGCAAGAAGCAGTATGCCTTCTATCGCGATTATCTGCTTAATCAAGAAAATATCCGAAAAATTTATGGAGCAAATATACCTTTCGAAACTTTTCAAGTAAAGGATATTTGTGAAATTAGAAGAGGTCGAGCGATCACTAAAGCGTATATTAGGAATAATCCTGGAGAAAATCCAGTTTATTCCGCAGCTACCACAAATGATGGTGAGTTAGGACACATTAAAGATTGCGACTTTGATGGTGAATACATCACTTGAACTACAAATGGTTATGCTGGAGTAGTTTTTTATCGTAATGGCAAGTTTAATGCATCACAAGATTGCGGTGTTTTAAAAGTTAAAAATAAAAAAATCTGCACCAAATTTCTATCCCTTTTATTAAAGATAGAAGCGCCAAAATTCGTTCATAATCTAGCTTCAAGACCAAAATTAAGCCAAAAAGTAATGGCTGAAATCGAACTCTCTTTCCCACCTCTAGAAATCCAAGAAAAGATTGCCGATATTCTCTTTGCTTTTGAAAAACTTTGCAATGATTTAGTAGAAGGTATTCCTGCCGAAATTGAATTGCGTAAAAAGCAATTGGATTACTATCAGAACTTCTTATTTAATTGAGTTCAAAAAATAAGAAATTAGAAAGTCTCAAATCTCTTTAATAATAAAAGTTAGTTCAGCAAACCACAAACTGATCCCACTTCTCCCCCACGCTCACCCGCTTCTCCACCGGGTTCAACCTCGCGGGGTCGGTGCTCGACCAGGTGTTGGATTATATTCCCTGGATTGGGAATGGGTACAGGTATGGCAATAACCACCGGGGCGTGGATGATATAACCGCGCCTCAAACCGGCGCGGGGTCGTCCAGCGGAACTAGTACGAACACAAGTGGTTCGCGTTCCTTTCTCCCGACGTTCTCGAATGTCGGCGTTGGTCTCAAAGCCAACGTCCAAGCCACGCTCGGTGGCAGTCAGACGACGACTACAGGCGGCTCCAAATGATCCACCCTCGACAAAGCCAACCTCCAGCCCTGAACGGGGGCGGGGTGAAGGAATGATAAGACTACTGGGAATAACACCCAAGGCAACGAAAACCACACCACCTTTAAAAACGCTACGGGGATGGGCAGTCAAAACCAGGGTTCTACAACAACTACCTCAGCGGGCAATCCCGATTCTTTAGTTACTGATAAAGTTGATCAAAAAGGTCAGGTGCAAACAAGTGGTCAAAATTTAAGTGATACCAACTACACCAACCTCCCCCCCAACCTCACCCCCACATCCGACTGACCGAACGCGCTCAGCTTCACCAACAAGAACAACGCGCAGCGCGCCCAGCTGTTCCTGCACGGCTTGTTGGGCAGCATCCCGGTGTTGGTTAATAAGAGTGGCGAAAATAACGAAAAATTCCAAGCCACCGACCAAAAATGGTCCTACACCGAATTAAAGTCGGATCAAACCAAGCTCAACCTCCCCGCTTATGGTGAGGTGAATGGGCTGTTGAATCCGGCGTTGGTGGAAACCTATTTTGGGACGACGCGTACGTCTAGCACTGCGAACCAAAACAGTACAACCGTCCCCGGTATCGGTTTTAAAATTCCCGAACAAAATAATGATTCAAAGGCTGTGCTGATCACCCCCGGGTTGGCTTGAACGCCCCAGGACGTTGGTAACCTCGTTGTCAGTGGCACCAGCTTCAGCTTCCAGCTCGGCGGGTGGCTGGTCAGCTTCACGGACTTTGTCAAACCCCGCGCGGGTTACCTCGGACTCCAGTTAACGGGCTTGGATGCAAGTGATGCGACGCAGCGCGCCCTCATTTGGGCCCCCCCGGCCCTGAGCGGCCTTTCGTGGCAGTTGGGTCAACCGGTTGGGCCGCGTGGAGAGTGTGTGGGATTTGAAGGGGGTGTGGGCGGATCAAGCTCAGTCCGACTCGCAAGGATCTACCACCACCGCAACAGGGGCTACCTTACCGGAGCACCCGAATGCTTTGGCCTTTCAGGTGAGTGTGGTGGAAGCGAGTGCTTACAAGCCAAACACGAGCTCCGGCCAAACCCAATCCACTAACAGTTCCCCCTACCTGCACTTGGTGAAGCCTAAGAAAGTTACCCAATCCGACAAAGTTAGACGACGATCTTAAAAACCTGTTGGACCCCAACGAGGTTCGCGCCAGAATGCTCAAATCATTTGGTACAGAAAATTTCACCCAACCCCAACCCCAACCCCAAGCCCTCAAAACAACGACACCGGTATTTGGGACGAGTAGTGGTAACCTCGGTAGTGTGCTTAGTGGTGGGGGGTACCACGCAGGGCTCAAGCACCACCAATCAACTGTCACCCGTTCAACGGGTGAGTGGGTGGACCGCTAACTATTAATAGGAACTTTTCTTATTAAGAAGAACTTTTTTGAAAAAAGTTTTGAAGTTATTTTGGTGTCTTTACTAACTTAAAGCAATTTAGTTAACGAATACCCAAAAAGTATGAAATTGATTAAATTAGCGAGGTTTCACCGCTTTTTAAAACAAGACCAATTGCAACTTGTTTCTTTGTTTAGCCAAAACTTTTTGCAAGTTTTGTTGACACCTTTAAATAAACCGGTCAATTTATTTAGTGAAAACACAACAATGAAAACAAATAAAAAATACGCCTTTGCGGCGTTGCTAATGCAAGCTCAAGCTAAGCTCCCAAATTCATTTAAATTCTTTCTTTCAAAAAAAGGAGGTATTACTTAATGAAAGAAAAAACTCCCTTTAAAAACGAAAAAGAATTCCATGAAATGGTGAAAAAGACCAAAAAGGGCACTTTTTCAGGTTGATATATCATTGACAAAGATAACAACAGTGTGGAATTCTCTGGCAAATTCAACAGACAATTTAAACTTAACAAACCGGTTATAACAGTGAACACGGAATACGTAACGCGTAAAGAGTTAAACGAGTACAAAGACTCAAACGACCAACGACTTACAAAGATTGAAACCACTTTGGCCGCCCAAGGTGAGCAAATCAACAAATTAACTCAAACTGTTGAAAAACAGGGCGAACAAATTAGAGAACTTCAAGTGGAGCAAAAAGCTCAGGGTGAGCAAATTAAAGCTCAGGGAGAAACCTTAAAGCTAATCTTGCAAACGCTGCAAAAAATGAGTGATCGCTTGGACAAAATTGATCCATCTAAATAGTTTTTAGGCTAATAACAAAAGTGCCAGCAGTTAATCTTAAAGATTAGTTGCTGGTTTTTTTCTTCACAACCAATAAACAAATAAAAAGCCATCCACCGCGAGGTGAACGGTAGATGGCTAAAAATAGTTTTTAATGATTTTGAAAACTAACGAAAAGTGTTAGTTTGAGTTGTAAAGCAGTTATGAATGTTTGTTCCTTTAAAAATTTGTTTTTTGTTTAGCTAAATATGTGCTGAAAGCCATTGCGTTAACATAAGTAAATTAAGACATAAAAAATGAACACATTAATCAAATAAGTGTGGGAACAGAAAATCAGGCTTTCAACACAAAATTTTCGTTTTTCTGCTCCCACTAAATAAATTGAGTTGGGTTTTAAAAACCGCCAAAAATTTTTAATTTAGTTTTTGCTACTTTCTCCTCCCTCCCCCTCACGAGTGAAAACCCCGGGGCGTGGGCCTTAGTGCGCGATAACACTGCCAAGGGCATCACTGCCGGCAGTGGCAGTCAACAAACCACGTATGATCCTGCGCGAACCGAGGCCACCTTGACCACCACCACCTTTGCGCTGCGCCGGTATGACCTCGCCGGGCGCGCCTTATACGACCTCGACTTTTCGAAGTTAAACCCACAAACGCCAACGCGTGATGCCAACTGCCAGATCACCTTTAACCCCTTTGGCGGCTTTGGTTTGAGTGGCAGTGCACCCCAACAGTGAAACGAGGTCAAAAACAAGGTCCCCGTCGAGGTGGCCCAAGACCCCACCGATCCTTATCGGTTTGCCGTTTTACTCGTGCCGCGCAGTGTGGTGTACTATGAGCAGTTGCAAAGGGGATTAGCGCTCCCTAACCAAGGGAGTTCGTCAGGCTCCGGTCAACAAAACACCACCATTGGCGCGTATGGGCTGAAGGTGAAGAACGCCGAGGCGGACACCGCAAAGAGCAATGAAAAACTCCAGGGCGATGAATCCAAGTCTTCCAATGGATCTTCAAGCACTTCCACCACCACCCAACGTGGTTCGACCAATTCCGACACCAAAGTCAAGGCTTTAAAAATAGAGGTGAAAAAGAAATCGGACTCGGAGGACAATGGTCAGCTGCAGTTAGAAAAAAATGATCTCGCCAACGCTCCCATTAAGCGGGGCGAGGAGTCGGGTCAGTCCGTCCAACTCAAGGCGGACGATTTTGGTACTGCCCCTTCCAGTTCGGGATCAGGCGGCAACTCCAACCCCGGTTCCCCCACCCCCTGAAGGCCGTGGCTTGCGACTGAGCAAATTCACAAGGACCTCCCCAAATGATCCGCCTCGATCCTGATTCTGTACGATGCGCCTTATGCGCGCAATCGTACCGCCATTGATCGCGTTGATCACTTGGATCCCAAGGTGATGACCGCGAACTATCCGCCCAGTTGAAGAATGCCCAAGTGAAACCACCACGGGTTGTGGGACTGAAAGGCGCGCGATGTTTTGTTCCAAACCACCGGGTTTGATGAAAGTAATACTTCGAACACCAAGCAGGGCTTTCAAAAGGAAGCTGACTCCGACAAGTCGGCCCCCATCGCCCTCCCGTTTGAAGCGTACTTCGCCAACATTGGCAACCTCACCTGGTTCGGGCAAGCGCTTTTGGTGTTTGGTGGCAATGGCCATGTTACCAAGTCGGCCCACACCGCGCCTTTGAGTATTTGGCTTTATATATATTTAGTAAAGGCAGTCACTTTTAGGTTGCTTTTAGCTAACTCTTTATTATCAAAAAGCAACATATATAAAAAAACAGCTAATTAAAGCTAGTAGAGCTAAGCGATTATTTCTTTTAAGTTAAATCTTAGTTGCTCAAGATTAAAACGCACTAAATTGAATGCTGTTGTAATAAGGCGTTTTTTCAAATCAAATTCATACACTAAAAGTGCGAAAAGGTTGTATACGCATGGAAAATGCGCGAGTTCTCTTAATTGTTTTCGGTATCGTACTAATTTTTGTTGGTATTGGCTTTTTATTTTGAGCATTAAGAATTACGCTTAAGCGTCAAAAAGGAGGCGAAGCTACTACTAGCTTAACGACTAACGATCCTGCTTTAAAGAACTTTGGTCGGCCAAGTTGATGGCAACGTAATAATCAACTTGTGCTCTTCCTCTTAGCGACAGTATTTTTGATGTTGGGAATAGGCGGTCTTTCTTCCATTCCCTCAATGTTTAAGTAACAAAAATTTAAGGATTTAAAAAAGGTTCATTTATGCAAATTAAAGCACAGGACACAGGACAGCAAAAGAAGTCCTGTTTATTGTCTAACATCCGCAATAAATGGAAAAACCGGAATCGCGGGAGTTTCCGCCAATGAGTTGGAAAGCTTTCCAATGGGTTGATGATCCCGATTGCCGTTTTACCTATTGCGGGTATTTTTTTGGGTGTTGGTGATGCAATTGCCGGAAATGCAGGTGATTTAACTGGTCTGCGTTATTTCGGACTTTTCATTAAAAATGGTGGTGATGTTGTTTTTGCTAACCTACCCATTTTGTTTGCTATTGCTATAGCAATAACGTTCTCACAAGACGCTGGGGTCGCCGGATTTTCGGCCTTTGTCTTTTGAGCTGCTATGAACGGGTTTATGAGCTCGTTAATATTGCCTTTTGATAAAGCAGGCAAAATTATTACTGACACTTCGACTCCAATTGCTGGTTTTAAAGTACTTTACTACAAGAGTGTCCCTGTTCATGCTATTGCTACAACCTTGGGACTGCGAACATTATCAACCTCAGTATTCGGTGGGATTATTGTAGGTGCCTTAACTTCAGTACTTTACAAGAAGTTTTATGCCATTAGGTTACCTGATGTAATTGGATTCTTTAGCGGTACTCGTTTTGTCCCTATTATTTGTTTTGTGGTAGCGATTCCTGTTGCGTTAATCTTGTTAATGATCTGACCAGCTGTCTCCATTGGACTTAATGCTATTGGTACTGGTTTAGGTTTCTTAGGTGGTAAAGGTTATGGTGCCAACAGTTTGATCTTTGGTTACATTGAACGTTCCTTAATTCCGTTTGGTGTGCACCACGCCTTCTATGCACCACTTTGATACACCTCTGCTGGTGGTAGCTTACAAGAAATTGTTAACCAACAGGTTTGGATTCGTCCCGACTTCCACTTAAGTGATAACTATGTAGCACGGGTAATTGGTTGAGTTGATCCTAATAACTCTAGCATGTACATTATTCCTGGTGCATTAAATGGTCAAAATGGAAGTTCAACCGGTAATACAATGTCAAAAGATCTTAATGGAGCACTTTCTGCCTACATGAGTAAAGAAAGCACAGCTTTCTTAACTTGAAAAGATCTAGTTGATGGCTTAACCTTTAAAGGTAATTTTGATAAGATGGCCGAAAACGGTTTGTTAGACGGTTCTAACAAAATTTGATTGGGTCTGAACGGTTCTGGTATTTTAGGTAAAAAATTGTTACTAAGTGATGGTAATGTTTACACTATTACCTTTAAAACCTTTGCCAACACTACTCCAATTGCTTGGAGTAAAGGTGCACAGGCAGTGTTACCTTTAAATGCTAGCTCTACAATCGTTAACAATCCAACCGCTTTAGCAGCAGCTACACAAAGTAATAATAACACTAACAACATTAAACTTTACCCGGTAAATTCCTTTAGGGTAGCTGTTGAAAGTTTAAATCCAGCGCAATACTCCCAGGGTAAATTCCCCTTCATGTTGTTTGGAATTCCAGCAGCTGGTGTAGCCATGATCTTGGCAGCACCTAAAGATAGAAGAAAGGAAGCAGCTTCGATTGTTGGTAGTGCAGCATTTACCAGTTTCTTAACTGGTATTACTGAACCATTTGAATTTACCTTCTTGTTCTTAGCGCCTTGATTATTCTATGGGGTGCACGCTGTTTTGGCAGCTGTAAGCTTCTGGTTAATGAACATCTTGGGTGCTAATGTGGGTCAAACCTTCTCTGGTTCCTTTATTGACTTTATTCTCTATGGTGCTTTACCAGATGGTCGTCGTTGGTTAGCAAACTCTTACTTAGTACCTATTATTGGACTGTTCTTAGCTGCCATCTACTTCCCAACCTTCTACTTCTTAATTAAGCACTTTAACTTGGCAACTCCAGGTCGTGGTGGTAAGTTAATTACCAAGAAGGAATACTTAGCAAGCAAAGCAGCCGCTAAAGCAGAGGGTGTAAGTGGTGTAGCAGAAAACTTTACCCAAACGCAAATTGAAGCGGGCATCTTACTCCAAGCTTACGGTGGCAAGGAAAACATTGTTGAACTAGGTGCATGTATTACCAAGTTACGGGTTACTGTTAAGAACCCCGAACTAGTTAAGGAAGAACCTATTAAAGAACTTGGAGCTGCTGGGGTAATGCGTACCACCCCAACCTTCTTTGTGGCCGTTTTTGGTACCCGCGCTGCCGTTTACAAATCTGCTATGCAGGACATTATCCAGGGCAAGGTTAACTGGGAAGCGTTGCAAAAGGTTATTAATACAGATCAACTAGCTGTTGAGCCTAAAGAAACCACTCCACCAAAGGAAGTAATGCCGGTGGTACAAGACGAAATTGTGATTCTGTCCCCAGTTAACGGTACCTTAAAATCACTCAATCAAGTACCCGATGAAACCTTTAAGCAAAAGCTAGTGGGTGAAGGTGTTGCCATTGTACCGAGCGATGGTCACTTTAAAGCCCCTGGTGAAGCAGGTGTGAAAACGGAGTTAGCTTTCCCTGGTGGTCACGCTTACATCTTTGACATTGATGGCATTAAGGTAATGTTGCACATTGGGATTGACACTGTGCAAATTAACGCCAAAAAACAACCGGGCGAACCACTAGAAGTGTTTGACATTAAGACCAAACAGGGTGAGTACACTAAGGAAAAGAGTGAAAGTGTAGTGGAAGTTGATTTAAAGAAACTCAGCAAGAAGTACAACCCAATTACCCCATTTGTGGTAATGAAGGAATCACTAGAAAACTTTAAATTAGTGCCAATCCGCCAACGCGGTGAGATTAAAGTGGGCCAACCAATCTTTAAACTAGTCTACAAGAAGTCACAGGCATAAAAACTGCTTGGCAAACAAAGAGGTCATAGGTAAGCCTATGGCCTTTTTGTTTATAATGAAAGTCCGGGGAAGGCTTTAGATGAATTTTTCCCTGGTGTTTCATCTGGTTATCTTTTATAAGTTTTAGCACCTGCAATTTTTTATGTTCTTGCACGCGTGTAGCAAACATCTTTTAAGTTTTTTCATTCTTCAACTTTAATTTCTTCAACAGTTTTCTAGTAGTTGCTTATTTTTCAAACTTAAGTTAGTTAGTTAAGTATTGCCTCTTTTTTTGAAGCAGCTACACTTATCAAGTTACTCATATTTTTCTTTTTCATATTTAGACATAGGTTGTGTTAAGGGATTTTTAGGACTATCCATGTAAATACATCTGATAACTTATTTTGATTTTGTTAGCAATAACACCAAAATCCACATCTAAAGCAAACCAAAAAGTCACCTGTTTTTAACGGGTGGCTTTTTAATTGTGGAACTGCAAGCTCCTTAATAATTCTTATTAAGGAGGATGACAATGTTTGGCTTAACACTTTGGCACAAAAATGGTGTTGGTTAGCAATCATTAATTAAGTTAACTTGCTCTGTTTGGTGCATAATGTAAAATTACAAAGCATGTCAGAACTGATTACAACACCTGTAGAAACCACCGCCAAAGCGGAACTCGTTTCCCTAGCTAAGCTCGGGGAAATGCGTACCCATGTCGGAATGGTTAAGCGTTACTGGAACCCCAAAATGGGCTTTTTTATTGAACCAGAACGCAAGCACAATAACGATCACTTTGTATTAGAGTTACAACGTCAATCTTTGCAAACAGCTTACAACTACGTCAAGGAAGTAGCACAAAACAACGGTCAAATTCTCTTCGTTGGTACTAAGAACGATTATGTGAAAAAACTCGTTAATAATATCGCTAAACGGGTTGATGTTGCTTTTATTACTCAGCGCTGATTGGGTGGTACTTTAACTAACTTTAAAACACTTTCAATTTCAATTAATAAACTCAATAAGTTAGTGGAAAAACAAGCGGAAAACGCGGCTGATTTAACTAAAAAGGAAAACTTGATGCTTTCCCGCGAAATTGAACGCTTGGAGAAGTTCTTTGGCGGGGTAAAATCACTTAAACGTTTACCGAACTTACTAATTGTGGATGACCCGGTATACGAAAAGAATGCGGTAGCGGAGGCTAACATCTTGAGAATTCCTGTAGTAGCGTTGTGTAACACTAACACCAATCCGGAATTAGTTGACTTTATTATTCCCGCTAACAACCACCAACCCCAATCAACCTGCTTGTTGATGAACTTATTAGCTGATGCGGTGGCTGAAGCTAAGGCAATGCCAACCATGTTTGCTTACAAACCGGATGAGGAAATTCAAATTGAAATTCCCCAAAAACAAGAGGCACCACGCCAAGTTGTAAACCGTGCAAATTCTAAACAAATTACCTCTCAACGGTTAAACATTACTCGCAACCCAGAAGTTCTCACCCGTGAATAAGTGAACTGGGTTGAGTGCTGCGGCCGTTTTAGAAAGCCGCGCACAGCATGGTGCTAATTTAATTCCTACAAAAAAATTAACCCCTTTTTGACTACTGTTCTTAGAACAGTTCAAGAGCTTAGTGGTGATCTTACTGCTGGTAGCTACCATCCTTTCACTAGTGGTTGCCATTATTAGTGGGGTTAATGCTAACTGGTTGTTTGACCACAACCTTGTTATTGAGTGAACCCAACCGTTTGTAATTTTGATAACGGTGTTGGCCAACTCCCTGATTGGCTCCATTCAGGAATTTAAAGCGCAAAAGTCAGCTCACACCTTAAAGTCCCTAACGCAACCCTTTACGCGGGTATTCCGTGAAGAAGGCTTGGTTTCGTTACCGGTGGGTGAAGTTGTTGTCGGTGACATTATCTTTCTGGAAGCGGGTGATATTATTCCCGCTGACGGGAAGGTGCTGCAAGCGAACCACCTCCGCTGTATGGAGAGCTTTTTAACTGGTGAATCAGTCCCAGTTGATAAAAGTGTGGTTAACACCGGTGGCAAGGGTTTGTTGGAACAAACAAACCTACTCTTTTCTGGTGCCCAAGTGGTCTTTGGTAGTGGTGTATTCGAAGTAACTGCTGTGGGGTTAAATACCCAAGTCGGTCAAATTGTCAAAACAGTGGACAGTAGTGCAACTAAGCTATCGCCCCTACAGCAAAAACTGGAAAAAGTGGGGAAGTGGTTTAGCTGGTTTGGCCTTGGTTTATTCGTTGTCGTTTTCCTAGTGCAGTTAGGGTTACTTGGTTTTCACAACTTTAGCGCTAACTGATCGATTGCCTTAATTGGTGCAATTGCCTTAGTAGTCGCTATTATCCCTGAGGGCTTAGTGACCTTTATTAACGTTATTTTTGCCTTAAGTGTGCAGAAGCTCACCAAACAAAAGGCAATTATTAAGTACCTAGCTGCAATCGAAACACTCGGTGGCGTGCAAATCATTTGTACCGACAAAACTGGTACTTTAACACAAAACAAGATGAAGGTCGTGGACTATTTCTGTTTTAGTAACACTACCCAAACAGACTTAGCACGTGCTTTGTGTTTGTGCAACAATGCTACAGTCAACACTAACGGAGACAGCACCGGTGATCCAACTGAAATTGCCCTTTTGCAATGGTTGGACCGTGATGGTTTGGAATTAAACCACTACACACGCGTGTACGAAAAGGCATTTGATTCCAACCGCAAGCTTATGAGTGTGGTGGTGCAAAAGGACAACCGTTTTATTGTGATTGTCAAGGGCGCACACGATGTCTTGTTACCGTTATGTAAGGGCTTGGATAGCAACCAAATTAAGCCTTTAATTGATGAACGTGCTAGCAACGGCTTGCGCAACTTAGCGGTGGGACTCAAGGTTTTATACTGCTTTGATCCAGAAAATACCCAAACTGTCAACGAATTGGAAAGCGAACTCGACTTTTTGGGTTCTGTTAGCTTACAAGATCCACCACGAATTGAAAGTAAAGCTGCCATTATGGCGTGTCACCAAGCCAACATTACCCCAATTATGATCACTGGTGATCACCTCAAAACCGCTACTGCAATTGCCAAAGAATTGGGCATTCTGACCGATGAAAGGCAAGCCATTTTAGGAGTCGATTTAGACCCCGCTAAAATTATGGAGTACCGCGTCTTTGCCAGAGTAACGCCGCAGCAAAAGCTAGAAATAGTTAATGCCTGAAAGCAAGCGGGTTACACGGTAGCCGTTACTGGTGATGGGGTCAATGATGCGCCCGCTTTAGTGACTAGTGATGTTGGTTGTTGTATGGGACAAACGGGGGTTGACATTGCCAAAGATGCCGCTGATGTCATTATTAGCGATGACAACTTTGCCACAATTGTTAACGGGATTGAACAGGGTCGTAAAACCTTTTTGACCTGTAAACGGGTGTTGTTTAACCTCTTTTTAACCTCAATTGCTGGCACGATTGTCGTGTTATTGGGTTTATTTGTATTAGGGGAAGTCTTTCGTGAACAACTCAGCAAAGCAAACCATAACTTCCAGGTCTTTACCCCTACGCAACTGCTAATTATTAACCTCTTTGTTCACGGCTTTCCCGCTGTGGCCTTAGCGATCCAACCCGTCCAGGAAAAACTGATGTTAAAACCGTTTTCCACCAAAAATCTGTTTTACAACCGTGGCGGGTTCGATTTAATCTGACAATCCTTGCTGTTGAGCTTTTTAACCCTACTTTTCTACAGTTTAGGGATGGTCTATGCCATTAATGATCCGGAGTTAGGCAAATCCGGTGATTTAATTAACCGGGCTGGTGCGACCTGTGGTTTTATGGTGTTAGGGGGCAGTGCAGCTTTAAATTCCTTAAACCTAATGGTGGATCGACCATTGGTAGCTACTAACCCAAAGCACTATGGCATTGTGTGACTAGGGGCGTTGTCCTCGATCTTTGTCTTCTTATTAATTATTTTCATTAACCCCTTGGGGTTAGTGTTTAGTACATTAAAAGACCTTACTGCCCACCCGGTTTTAATTGGTTATAGCTTTGGGGGCGTGCTTTTATATATGACAATTAACGAAGTTGTTAAACTTATTAGGTTAAGTTATGGGTCTGTTTAACTTCTTGAAGTTAGTCTCCCCGCGTCATCGGATTTACCATAAGGCAAGTAAGATTGCCAACGAAGTCGAGGGACACAAAAATTACTACCGCAACCTCACTGATGTACAGCTGCTGGAAGAGTCCAATAAGCTAGTGGATTTAGTGACAAAGCAAAACTACACCATTTTGGATGTGGCGGTGGCTGCTTTGGCCTTAATTCGCGAAGTGGTCTACCGTGAAACCGGGGAGTTTGCTTACAGAGTGCAAATTATTGGTGCTTACATCGTCTTGATCGGTGACTTTGCTGAAATGATGACTGGGGAAGGGAAAACCCTGACCATTGTTTTAGCAGCCTATGTCTCGGCGTTAGAAAAACGCGGTGTCCATGTGGTTACGGTCAATGAATACCTAGCGCAGCGGGATGCTACTAACGCCACCAAGATCTTAAAGCGTGTTGGCATGACCGTTGGTTGTAACTTTGCTAACCTAGCACCACATTTAAAGCAAGCCGCGTTTGCTTGTGATGTAACTTACACCACTAACAGTGAACTCGGTTTTGATTACCTCAGGGACAATATGGTGCACCGCTTTGAGGACAAGAAGATCCGCGAGTTGCACTTTGCGATAGTAGACGAAGGCGATTCGGTTCTCATTGATGAAGCGCGCACCCCTTTAATTATTTCTGGTCCTGCCAAAAACGAGTTTGCAGCATACGTGGCTGTTGATCGCTTTGTCAAAAAGCTCAAGGAAGACGAGTACAAGATTGACCCCGAATCACGTGCCCCCGCTTTAACAGAACTTGGCATTAAGCACGCGGAAAAGAACTTTAAAACCGATAACCTGTTTGCGCTAGAAAACAGTGACCTGTTCCACAAGATTATTAACGCTTTAACCGCCGTTAAAGTTTTTGAGCAGGGCAAGGAGTACATTGTGCGTGATGGTAAGGTGTTAATTGTGGACCACTTTACTGGCCGAATCCTGGAGGGCAGGAGCTACAGTAATGGGCTCCACCAAGCGGTTCAGGCCAAGGAAATGGTCGAAATTGAACCAGAAAATGTGATAGTAGCTACGATTACCTACCAATCGTTTTTCCGTCTGTACAACCGGTTGTCAGCGGTATCGGGTACCGCTTTTACTGAATCGGAGGAATTCCTCAAGATTTACAACATGGTGGTAGTACCCGTACCTACTAACCGACCCAATATCCGCAAAGACCGTGCTGACAGCGTCTTTGGTACGCCCAACATTAAGTGGCTAGCAGTGGTCAAGGAAGTCAAACGGATCCACGAAACCGGGCGACCAATCCTAATTGGGACCGCCAACATTGATGACTCGGAACTACTTCACAACTACCTCCAAGAAGCCAACATTCCCCACGAAGTTCTCAACGCCAAGAACCACTCTCGGGAAGCGGAAATAGTCGCTAAGGCGGGCCAAAAAGGGGCGGTGACCATTTCTACCAATATGGCGGGAAGAGGTACTGACATTCGTTTAGGTGAAGGGGTCGCTGAAATGGGCGGGTTGTATGTCCTTGGTACGGAACGCAACGAATCGCGCCGGATTGACAACCAACTGCGCGGTCGGGCGGGTCGGCAAGGCGATCGCGGTGAGACCAAGTTCTTTATCTCCTTGGGTGATGCCCTGTTCAAACGCTTTGCCCATGACCGGATTGAACGCGCCATTACTAAGTTAGGGAACGATACCTTTGACAGCTCCTTCTTTTCCAAGATGTTAAGCCGTACCCAAAAACGGGTCGAAGCAATTAACTTTGACACCAGAAAGAACTTAATTGATTATGACCATGTGTTGGCCAGTCAACGGGAATTGATCTACAAACAACGCGATAAGTTCTTATTAGCCACTGATCTCAGTGACATGATCGACAAGATGTTGGAAAAGTTTGTCGAGCAGTTCTGTGACCAGTACCGCAACCCTAAGAACCAAAACCTCGTTAACCACATTGCTTTATCAGAGGCCTTAAACCTGGAATTAAACATGCACGGTGTTATTAGTCCGAAGCTGTTTGAAAACATGACTTTTGATGCGACTGTCCATAAAACCCACAGTTTAATTGGTGAAAAGATTACCAATAAGGTCAAGGTCTTAACCCCCCCAATTGCCCTCATCCGTTTTCGTGAAATTATGATCACAGCAATGGACAAGCACTGAATCGAACACTTAGACAATGTCTTTAAGCTCAGGGAAGGGGTTACCTTGCGCTCGATGGAACAAACGAGTCCCCTCAACGTTTACATTCGCGAAACCGACATCTTGTTCCAAACAATGTTGCAAAAGATTGCTCGGGACGTCATTATCCAAATTGCTAACTTAGCGACTCCCGAAGAGTTTGATGAGGAACTAATGAAGGCTAACGCCTTGAAGAAGTTGCAAGCACTTAGAGAAGCTCATGAAAAGTCCAACGAAGGACAGTAAACTGTTCCACCTCAAGAGTAACTTTGCCCCCACTGGGGACCAACCAGCGGCGATTGCTAAGTTAGCTGAGTTTCAAACCAATGAACAGGTCTTACTCGGTGCTACGGGTACCGGTAAGACCTTTACGATTGCCAACGTTATTCAAAAGGTACAACTGCCTACAGTAGTGATTGCCCACAATAAAACGTTAGCGGGACAGCTTTATCAGGAGTTGAAGGAGTTATTCCCTAATAATGCGGTGGAGTACTTCATCTCCTACTTTGACTTTTACCAACCAGAGGCTTATCTGCCCGCTAAGGGGGTTTACATTGAAAAGAGTGCAACTGTTAACGAAGAGATTAAACGGTTACGAGTGTCCACCTTACATTCACTCTCTACCCGCAAGGATGTGATTGTGGTGGGTTCGGTAGCCAGTATTTACCCAACTTCTTCTCCCGCTGATTTCGCCCAGTACTCGCTCTGGTTAGTAGTTGGTAAGGAATACGGTTTAAGTGAGTTAAAAACGCAGTTAATTCACCTAAACTATGTTGTTAATAAACAACAGTTAACTCCGGGAAAGTTCCGCTTTCAGGGCGATGTTGTTGAAGTGTTTCCTGGTTATGCGCAGGATTATGTCTTGCGGCTTTCCTTCTTTGACCAGCAACTAGAACAAATTGCTCGCATCGATCCCTTAACTAACAAGGTATTGGAAACCCTTAACAGCTTTAAGTTAGGTCCCGCTGATGAGTACATAGTAAACCAAAATGACTTAGGAGTAGCCTTAGACACGATTAAGGCCGAACTCAAAGATCGCTTAAAGTACTTTGAACGGTTAAACTTCCCCGAACGTGCCCAGCGCTTACAAACGATTACCGAACACGATTTAGCAGATCTGAAGGCCTGGGGGGTATGTAGTGGCGTGGAAAACTATGCGCGGCATTTAGAACACCGCCCACCGCACTCCAAGCCGTATAACATCTTTGATTACTTTACTAAAGGTGAATGGCTATTGGTAGTTGATGAATCCCACCAAACGCTACCGCAAATTAAGGGGATGTACAACACCGATATTTCACGGAAACAAAGCCTAATTGAGTACGGTTTTCGCCTCCCTTCAGCTTTAGACAACCGTCCTTTATCCTATGAGGAATTCCGCCAGGGAATCAACAAGGTCATTTATGTATCCGCTACCCCTAGGGAAGAGGAAATTCAGTTAAGTCACAACAACGTGGTCGAACAGTTAGTGCGTCCCACCTACCTCTTGGACCCGGAAGTGATTGTGAAACCGAAGGACAACCAGGTTGAAGACCTAGTGAGTGAAATCATTAACCAAAGAAAACACAACGGTCGTACCTTTGTCACGGTGTTAACGATTAAGATGGCGGAAAACCTGACTGACTTTTTGAAGGAGCGCAACATTAAGGTAGCGTACATCCACAAGGATATTAAAGCCTTGGAACGTTTAATTCTGTTAACGGATTTACGCAAAGGTGAGTATGAGTGTTTAGTGGGGATTAACTTACTGCGAGAAGGTTTAGATGTTCCTGAAGTTTCACTCGTAGCCATCTTTGACGCTGACATTCCTGGATTACCACGCGATGAGCGCAGTTTAATCCAGATAATTGGCCGCGCTGCGCGAAACGTTCATGGCCGGGTGATTATGTATGCCAATACCATTAGCGAACAAATGGATAAGGCGATTAAGGAAACGCAACGCCGGCGCACCATCCAAATGGCTTACAACGAACAACACCACAAAACACCAATGACGGTGCAAAAACCAATTACCTTAAACCAGCCGATTAAGTTAAAGACCAAAAGCTCAGAGCAGCAAAAAGCTGCTTTAATTAAGCAGCTAACCAAGGAAATGAAGCAAGCAGCAGCTAACCAAAACTATGAGTTAGCGATTGAGATCCGCGACTCGATCTTTGAATTGGAAAAGCAATTTCGTGGTAAAATTAAGAGCTAGCAATGCGTAAACTAATTAAATTAAACGTCATTGTCTTTGTCTTGTTGTACTTGGGCGAGCTGTTTGCCAGCCTTTGGTTCAAGTTAATCAGTTGCCTCAAGACACGCAACCAGTACTCCTTAAACGGGTACTATGCGTTGTTTGTCTTTGTCAACATCATCCAAAAGATGGCTAACTCTTTCCAAAAGTTAGCTTCCTCAGTTGTGTTGTTTGAAACTGAAATTAACGAATTTTTAGTTCTCTTTACTGATACAAAGAATAAACGTGAGGAGAGTGAACCAGTGCGCCAGGTGTCAACAACCCAAGAGTATCACCAGGTTACGCTCGACCAACAACACTACTTTAACCACAAACTGAGCGATTACTTCCGTTTGTTTAAGGACAAAACTTTCTTCTTTGAAATTATCTAGTTACTAAATTGACCTGAATTGCAACCTTTCAGGCACTTTTTACTGTTAGTAACTAGTCTTTTTTCATTTCAATATTTAAGTTTTTAATTTAACCAATTTTTACTATGAAGCTTAGTGCTATTATCTCCCTATCAGTCGCTGGTACTGTGGGAACAACTGCGGTGGTAGTACCTACAACTATAACGCTTGTAAATAAGACCCACCAAGTAGAACATGAATCAGAACAATCGGATTTTCAAGATATTCGCTTTGGTCTTAATAGTGTTAAGTTGCCAAAAGCACAGCCAGCTGCGGCAACTAGAATTACCGTGGAAAACGGGACTGATAAATTAGTCAACTATAAGTCCTCACCACAACAACTCTTTTTAGCGAAGAACGCGCTTAAGGATAAACTCCAAGGTGAGTTTGATAAATTCCTAAGTGATGCGAAGGCCTTCCCAGCGCTAACCGCTGATTTACAGGAATGGGTTGACCAACAGCTGTTTAATCCAAACCAAAGTTTCTTTGATTTAAGTGCGCCCAGGTCAAACTTTACCCTTTCATCTGACAAAAAGGCTAGTTTAGACTTTATCTTCCGCTTTACTAACTTCACCGAATCCGTTCAGTTGTTAAAACTACCAGAAGGTGTATCGGTTGTAGTTGACTCCAAACAAAGCTTTGATTACTATGTCAATGCTAGTGCCCAAAAATTATTAGTTCTACCGCTGTCTTTACCAGATTACACTTTGGGTTTAAACTATATGTTTGACCACATTACTTTAAACGGTAAGGTTGTCAATAAATTTAGTTTTAATCCGTTCAAAACGAATTTAAACCTCGCCTTTAGCAACGTTTACAATGGCGTTGATGTGTTTGAAGCACAAAAGAATTTAGTAGGTAAGGGTAAATACCTAAACACCCACGTGAAGGCTGAAGACGTAAAGAAGGACGTTAATGCCAACATTAAAAACCAATTTGACATTGCCAAAATTATCGCTGAGCTAATGGGTAAAGCCCTTAAAGAATTTGGCAATCAACAAGAAGGTCAACCATTATCCTTCCTAAAGGTAATGGATAAAGTTAAAGAAGATTTTGAAAAACTGTTTAACTTAGTCCGTCCTGGATTGGGTAAATTTGTTAAGGACTTAATCCAAAGTAGTAGTCAAGCAGAAAACAAGATAACTGTCTACAAGTTAATCTTTGACAACAAAAAGACCATCTTAAACCTACTTAAAGAGCTTTCCATTCCGGAATTAAACTCTTCTTTAGGTTTAGTGGACGTCTTGTTTGATGGCATTACTGACTCTGATGGTCTCTATGAAAGGTTGCAATCTTTCAAAGACTTAATCGTTCCAGCAGTTAAAACGAATGAAAAAACCGCGGCTTTAAGTCCATTAATTGAAGAGTTATTAACCCAAAAGGATACCTATGTGTTTGACTTAATTCAAAAACACAAGGGTATCTTGACTAACTTGTTAAAGAACTTCTTAGCTGATTTCCAAAAATCAACGCCGTTTATGGCTGATCAAGTAGCCATCTTCACTGAGTTATTTGACAACGAAGGTGCGTTTGATTTATTTGGTGAGGCTGACTTTGTTGACAAGATTGCCGAACTCTTCTTAACAAAGCGTACTGTTAAAAATGGTGAAAAAATTGAAACTAAAGATTCCCTACTGGTAACATCATTAAAGAGTCTTTTAGGGGAAAAGGTAGCTGCCTTAGGTGATTTGTTAGATAGCTACATCTTTAAAAATGAATTACTTAACCGCAGTGTAGAAGTGGCTAAGGCTGAAGCTAAGGACACTAAAGGTGCTACCGATTACAAAAAGGAACAAGCTAAGGCGCTCAAAAAACTCTTTAAACACATTGGTGAAAACACTTTAAGTAAAACCAATCTCGATAAAATCACCTTAAAAGAAGTTAAAAACACCGAAAATGTTGAATTAGAAGAAACTGAAACAACCTTAAAGGTTAAAAAACTCGATGTTGAATACAAGGTAGAACTTGGCAACTTTGAAATCAAGAATGGCTTGATTAAGGCAATGCTCGAATTCTTGCCAGACACTAAAGATTTAGAAACAACTTTAGATAAACTCTTGTTCAAAGGGGAAAGCTACAAAGCGATGAAAGACAAGTACATCAAGGAAGGTTTCCCTGGTTATGGTTGGGCTAAAGGAGTAGTCCCTGGGGCCTTTGAATCTATTGAAAATACTTTTAAGAGTGCTATTGATAAAACCAAGTCCATTCGTGATCTCTTTGGCGACATGCTCTTTGGTAACGATTTAAGCAGCGTTAAAGAAACTGATTCATTCATCACCCTAGGTGGTTCGTTCGACATTAAGTATGGCGGTGAAAATCTCAATGTGTTGCCAGCTTACTACTCTTTAATTAACAGCGAAATTGGCTATCAAATTATTGGTGTAGATACCACAATTGATGCAACTAAGGTAAAAGTTGAATTAAAAAACAAAGAGTATAAGGGTAAATCGCCAGCTATTAACGGTCAGGTGAAGTTGTCACAATCATTCTTTAATGTTTGGACAAATATGTTTGACAGCATTACCAAACAAATCTTCCAAAAGAAATACGAGTTCAAAGATAACATCCAAGTGTTTGCGCGAAACGAAGATAACACATCACGTTTAGAACTTGACATTTCTGATCCTGAACAACGGGTAATTCCATTTGCTTTTGTTGATGGCTTTGGCATTCAACTCAAAGCAGTTGACAAAAACATTACGAAAGAAGCAGGTAATACTGAGCCAAAATCTCCTGTAATTCAACTTTATGAGGCACTTAATAAAGAAAAAGATCAAAAACAACAAAGTAAACAATCTCCAAAACAACTTGATACTAAAACACAGTTGGGTTACCTATTGAAATTAGGCGACAATTGGAGTAAAGATGATTACAAAAGCTTAATTGATGATACGATCATCAATAACAACTATTTAGAGGCCAGCTTTAATTCCAAGATAACGGTTGATCGCTTGGGTATTCCTATTGACCTTTGATTGTTTAAGATTTGACCTAAGTTCAATTTGGAAATCCCAATGCAAGGTTCCTTACAACTTTACAGTAGTAGTGTTATCTTCCCATACGGTATTTATGACACCAGTGTTCAAGATGCTGCGAAGATTGTGAAGCGTCTGAACTTTACTGACATGGGCTTCAAACTCAACGATCCAAAACCTAACTTCTGGTTCGTTGGTTTTTAGAGCAGACTAACTACTATACTGATTAAGCTACCTAGAAGGTAAAGCGAGAAGTTCATCCACCAAAACCCGCGGGTTTGTTTACGGTAGGATGAACTTTTTGCTTTATACTCGTCAAAACTAATGTTGGGCTTGGCGTATATACTACCTAGTTTTAGATATTGGTTAAACTTGCTAATTTCGTACCGTACTCCATCACAGGTTTTTAAGCGCACTATACCTTGGAGACACACAAGCAATCAAACAACGACAAAACTAACAACACAACTGTTTTTCAGAATTAGGTTAATGTTGTTAGTTTTATTGAAAAAAACGGTTAAATTCAAAATGGCGAAGAAAAGCACAATGTAAATTGTGCTTACTATTAACCCGCCGTATTCACGCGGCTTCGAATTTGTGGTTTGTGTACCTAACATGTACTTACTAGTATCGTTTTTTTGTAGAATGTTTTGAACCACTAACAATGTTTTTAGCGACTAAAATGCTGTTTGACTCCGAGTGGAAACCGATTATAAATCGCGTTTCCCTGTAGCCATCTGCACTTTTAGTTGGACTGGTTACATCAAATCTTTCAGGTAACCAGAATATTAGCATGCTGAGGGACCTGAGAACCAGGTTCCTTTTTCTTTTTTATTAGTATTCGAAAGTGTTCAAGTATATTCTTAAACGTTTGGGTTTGGCAGTGTTAGCGATGTTTATCGTGATGACCATTGTCTTCTTTTTGGTTAACTCCACAGGTCAAACGCCCTTATCAGCAACCTCTTCTAAGGATTTGGAGGCTGTCAAAACACAACTAGATGCCTTTGGTTTTAACGATCCGTTAATTGTGCGATATGGTCGTTACTGACAAACCCTCTTTTCAGGTAGTTTGGGTACTTATTACAGCTCCCCGAACCAAACAATTGACCAAATTGTCTTTGGTCGGGTACCTAACACCCTTTACGTTGTTTTAATTTCTTTCTTTATCGGCTCTTTGTTGGGCATTATCTTCGGGATGATTTCCGGATTATTCCGTGGCAAGTTAATTGATGCGGTAATTAACGTGCTCGTTGTTTTATTTGTTTCGATTCCTTCCTTTGTGGTTGGTTTAGGTTTACTTAAGGCAGCTGGTTTGTTCCGTTTACCACCGCGCTTTATTAACTTCGATGATGCTAACTTTAACTTTGGTAACTTCTTGTTAGCCTCCATTATTCCTATTTTATCCTTGGTATTTTACACCTCAGCGGCCTTTACTTATCGGGTACGGAATGAGGTCGTGGAAGTAATGAACCAGGACTACATAAAAACAGCACGTAGTAAAGGTTTGAGTACTTTTGCTGTAGCGCTTTACCACATCTTCCGTAACTCAATTATTCCTTCTGTACCTTTATTTGTCTTTGGGATCTCTGGTGCCTTTTCGGGTGGTTTTATTATTGAATCCTTATTTGGTGTTCAGGGGGTATCACGGATCTTAATTGACTCGGTGCAAAGTAACGAAACTAACCTCGTGATGTTTAACATTATGTTCATCCAGGGGATTCCCTTACTAGCAAGTGTCTTCATTGAGTTGATTTATGTCCTAGTGGATCCTAGGATTCGGATTGCTAGTGCCGGTGGGGTGAGTCTGTGAACTAAGCTTAAGTTTGTTTATTTACGCCAAGCTTGATTGCGTAAGTGAAGACGGATTAACCACACTAATTCCCACAATGTGTTGTTCAATTCGCCCCAACACCGCCAGCTGTTAGAGCTCAAAGCAATTGATTACAAGCACAATACCATTAGCTTAACTGAACAGCAAAAAACCACCCTTAAAATTGAACCAACTGCTAACTTTGTTTTGTTAGGAACTAAATGTTTAAAGATTATTACGATCCATGGATAAGCACCAAAAATTTGACCAAAGCCTGTTTCAACGGGTAGACATTAATGTCTTAAAACGCTCTGACCAGTTGATTGGCAAACCAACCACTAACTTTGTCGAAATTATGAAGCGGTTGTTCCAAAATAAGTGAGCAATCCTCTTCTTTTTACTGATTGTGCTAATTATTTTGTTAGCCATCATTGTACCCTTAGCATCACCTTATTCAGCCGTTACTCCAGTATCGAACAACGCTTTGGCACAGAACCTACCACCACGATACCTCTGGAACGGCGCGGGTGACATTCAAGTAGAGAAAATTACGGCACGCTCGATTGCTGAAGTGGCCCAATCAAGTGGTGTTTTGGTAGGGAAATTACCGGAGGCAAGTAGCAATCCACTCGCTACTAACGTTAAGTACAACATTGCGCCTTACCAACTAGCAGAGCTGAAAAACTACTATCCATTATTAGGCACTAACGGTTTAGGGGTTGATATTTGAACTTTACTGTGAGCCAGTATGGCCAAGTCACTCTGAATTGCGATTGTGGTAGCCTTAGTTTCAATGGTCTTCGGCACCATTTACGGCGCGATTGCTGGGAGCTTTGTTGGACGCGCTGCTGACAACATTATGAGCCGGATCATTGAAATTATTGATTTGGTTCCTTCAATTCTCTGGATCATTGTCTTGGGAGCAACATTCCGCTTTGGTGGGGTAAAACAATTTGATGACAGTGTGGTGATCTTTACCTTAATCTTTGTCTTTTGGACCTGACCCGCTGCTACCACAAGGATCTACATCTTAAAGAATAAGGATACGGAGTACATCCAAGCAGCGCGTACCTTAGGAGCAAAACAAATTCGCATTATTTTTGTCCACATGCTACCAGTTGTAACTGGTCGTTTAGCAGTCGTCTTTGTGAGTTTGATTCCCGCTGTGATTGGTTATGAAGCATCACTGGTTTTCTTAGGGCTTAAACCTGCTACCGAAGTGGGACTTGGTGCTTTATTAAACCAGGTCACCAGTAGTGGTAACATAGCACTGATTACCAGCTCAATTGTGAGCTTTGCAATTCTCACCGTTTCGACCCGCGTGTTTGCTAACGCTTTAAATGACGCAATTGACCCACGGGTAATAAGGAGGTAATATGGCACTAAAAGCAACTAATTTTTTTACAGAACCAGAGTACAAACTGCAGGATGATTTGATCTTAGATATCCGTGATCTTCACGTGAGTTTTAAGGTTAAAGACGGCATTATGCAAGCTGTCCGTGGTGTTGATCTCAAAGTCAAAAAGGGCTCCATTGTCGGCATTGTGGGCGAATCTGGTAGTGGTAAATCGGTCTGTGTTAAGTCGATTATTGGCTTTAACGATGGCGCAAAAACGACCGCTAAGTTAATGAACTTTAAGAACATTGACATTAGCAAGATGAAAAAGCACCAGTGACAGTACTACCGTGGCACGTACGTGTCTTACATTTCCCAAGATCCACTGTTCTCGTTAAACCCCACCATGACCATCGGTCGCCAGGTTAAAGAAGCGATTTATGTGTCGTGTAAGCGCCGATATTACCAAACTAAGAGTGACCTCAAATACGATTTGCAAACAGAACGAATTGACCTGGACACTTACAAGGAAAAACTAGCACAAGCCAAGGAAACTTACAAAGCTAAAACGACTAAGGCGGCGGTCCATGCAAAAACGCTAGAAATCCTTAACTTCATTGGCATTGACCAAGCTGAAAAGCGCTTAAAGGCCTTTCCAAGTGAGTTTTCCGGGGGAATGCGGCAACGGATTGTGATCGCGATTGCCGTAGCCACCGAACCGGATTTAATTATTGCCGATGAACCGACCACGGCGTTGGATGTGACCATCCAAGCGAAGGCGTTGAACCTAATTAAACAGCTGCGTGACCTGCTCAACATTACCATTATTTTTATTAGTCACAACATCTCGTTAATTGCTAATTTCTGTGACTTTGTATACGTAATGTACGCTGGTCGATTAGTGGAAAAGGGCTTAGTTGAGGAAATCTTTACCAATCCGGTGCACCCCTACACTTGGGCATTAATGGCTTCTATCCCTGAAGGTACGGACAAAAACGCTCCGCTAACCTCAATTCCTGGGTATATTCCCAATATGTTATCCCCGCCTAAGGGCGATGCCTTTGCAGCGCGGAACCAGTTTGCACTTGCCATTGATTTCGAGCACCAACCGCCGTTCTTTGATGTAACGGAAACTCATAAGGCCGCTACCTGGCTACTCCATCCCCAAGCACCGAAGGTAGAGATGCCCGCCGATGTCAAGGAAAAGATTGCCATTACCCGCAAAGCACTCGCCATTAAAATGCCATCTCAACCTGTAAAACAACCAAAAAGCAATGGAAACAAAAAAACAAAAACAAAATCCGCTCGTTAATGTTAAAGCCTTGAGCATGTTGTTCAAGGTCCGCGGTAGCTTCTTTAAAGCCTTAGATGAAATTGACTTTACCGTCAATGAAGGTGACTTCTTTGGGGTCATTGGCGAATCGGGTAGTGGTAAGTCAACCACGGGGAAGTGTTTAATCCGGTTAAATATCCCGAGTGGCGGTAAGGTGGAAATTGCTAACCACCTTATTTCTGGTAAAAAACTAACACGCGAAAACGACCACTGGTTAAAGCAAAACGTGCAGATGGTCTTTCAAGACCCATACTCTTCTTTAAACCCTACCAAGAATGTTTTAACGGTCATTTCCGAACCGTTAGTAATTACCAAAACGGTGTATGGTGAGGTAAAGGAATATCTTAAAACGCTCGCTAAACTGTCCTTTAAAACCAAGAAGGAATTGTTAAGGGAAGACTTTGAACTGGAAACCCAGTTTTACGAAAAGTTCTTTTCAAAGGTACTGTTTCACTTAGAAACTACGATTAACAAGTTTGCCCTACTCCAAGAGAGTAATAACAATAGTTCAGCCGAGTTAGCACAAACCATTTTGGGTCACACTGATGATCTAATTGAAGCATTACGGCAAGAGTTCGGTCTGGTCTATGAGTTTTCCTCATCCCAAAGCGAACCGCTACAAAAGGCCCTCAAAGACAAACAGGAAACCTTAGCACAGGACACGATTGACAAGTTAAAGCAAGAGCTGTATACAACGCAGCAAAAAGCTAAAGTTTCCACCCAGGCCTTTGCTACTTGACAAAAGTTGCAACAAACTAAGCAGAACTTAAAAGCGTACCGTGCCCAAATGGCGGAAGAGTTGCAAAACAAACCACGGATTTACCTCAATGCATGGTTGTTAACAACGAAGAACTACATTAAGGATTCACGCCAAAACACGCAGTTAACTGACGATGTCTTTGCTTTTTCTTACAACGACATGGTCGACAAAAAGCGCCGCTTAGTGTTAGTGTTATCGGAGTATTACAAGGCGTTGCCGTACTTTTATGACAATTGGATCCACCAGAACGCCGATCGTTTTGATGAATTGACCAACGCAGTCTTCTTTGACTTGATTGATGTGGTGATTGCCTTAAACCGTGATTTTGCTAATGTGGAGAGTGACGCTAAGGCCGAGTTAATCCGTTTTGTTCAGTTTATCCGTCGCTTGTGTGACTTACGCTTTGCTGCTTTGAAAAAGAGTTTTAAGAAGCAAACTAACTATAGTTTTGACTTTAACCGCGAAACCGAGTTGTTATACGCCAACAGCTGTTATGACATTAAGGAGCTCCCCCAAGTAATCCAACCATACTGGGAAAAGCTCTTTAGTGATGCTAACTATGACAAGATTGCCAAATCAGTGCAGGAACTCAACGATATTATTAGCACTGACATTGAAAAGGCATCGAATATAGCCAGTGAAATTAATACCAAGATTAGTAGTTTTAAAACTGAAATTGCGGAGTTAAAAGCTACCTTTAAAACAGAAAAAAAAGCAGAAGATCACAGTGCCCAAATTACTGGCTTAAAAACGCAAATTGCTGAAATTCAAACGCAAATCAAGCAGCAAAAACGCGAAGTCCAAAGTACCGAAAAGGCCGCTTTAAAACCAGTATTAAAGCAGTACAAGAGTGCGCTGCACCTCTATAAACGCTTTAAACAGTTGTTACGCCAATTTACCAAGCAGTTAAACCTCCTTGTCAAAAAACAGCAGGAATTAGAAAAGATTGAGGAAGGCTTGGATCTAACCATTTGAGAGCGGATCCAACTGTTGTTCTACCCGGTCGAAGGTGATCTCAAGAGTGAACTCAAAACACGCTTAAAGTCGTTTGGGGTGATTAACTTTGAGTACAAACGTGCTGTGCGTGAATCCCGTGTCTTTCGCTTAGTCCACTTTGGTCATGACGTGATGAAGTGAGGATTGTTCTTACCATTGACCAAGATCTTCATGCGTAACAAGGTGTATGAAGCATTAGACAGTGTGGGCCTCAAACGCGAGCACGCTTATCGCTATCCGCACGAGTTTTCTGGGGGTCAGCGGCAACGGATTGCGATTGCCCGCGCTTTAATTACCAAACCTAAGTTAATTATTGCAGATGAGCTTATTAGTGCTTTGGATGTATCCATTCAAGCGCAGGTGATTAACATCTTGAAGGACTTGGCTAAAAAACACAACTTAACGGTGCTCTTTATTGCCCACGACCTTTCGATGGTACAAACAGTGTGCAACCGTTTAATTATTATGCACCGCGGCAAAATTGTGGAACGGGGTAGTACGGATGAAATCTTCGCGCACCCGGTCCACCCTTACACCCGTTCCTTGATTAAGGCATCGCCAAAGTTGAGTAAGATTAACATTGACCTAGCCTCCTTTGACGAGAAGTTTACGTATGACAGTGATTATTCACTCACGAATATGCCAAGCTTCTTGAAGGTGCCAAATACCCAAGAACACGAGTTGTACTGTACTCAAGGCCAGTTTGACAGTTGGATCAAAGGAGCAAGTCGGATAAATTAATTTAAAATTTCTAGCAAATTGTGGGAGCTAGTTTAGCGTTATTACCACACCAATTATGGCAAAAAAGACTATTACAAGAATCGCTAAGATTAACCTCTTAGGCGGGCAAGCAAAGCCCGGTCCCGCACTCGCATCTGTTGGGATTAACATGGGTGAATTCACCAAACAATTTAACGAAAAAACCAAGGACAAACAGGGCGAAATGATCCCCTGTGTGATCACCGCTTACAACGATAAGTCGTTCGACTTTATCTTAAAAACTACCCCAGTTAGCATCTTGCTTAAGCAGGCTGCAAAACTGGAAAAGGGGGCGAAAAACGCCAAAACAATTGTGGGTAAGATTACGATGGCCAAGGCCAAGGAAATTGCGCAGTACAAGTTAGTGGATCTGAACGCTAACACCGTGGAAGCAGCCCTTAAAATGGTTTTAGGTACTGCTAAACAAATGGGAATCGAGGTGATCGAATAATGGCTAAACTATCAAAGAAAATGAAGATAGCTGTTGGTTTGGTCGATAAAACCAAGCTGTACCCGCTCCAAGAAGCAGTTGATTTAGTCAAAAAAACTTCAATTACCAAATTTAATGGCTCAGTTGATATTGCCGTTAGCCTTAATTTAGACACCACCAAGGCGGAACAACAGCTCCGCGGTGCGATTGCTTTTCCTCACAGTGTTGGCAAACCAATCCGTATTCTAGCGATCACCGATGATGAAAAGGCGGCACTGGAAGCAGGTGCTGACTTTGTTGGTGGCATTGACAAGATCAATGACATTAAAAACGGTTGACTAGACTTTGACCTGATCATCACTTCTCCAAAGTTTATGGCCGCATTAGGTAAACTCGGGAAGCTGTTAGGTACCAAGGGTTTAATGCCTAACCCAAAAACAGAAACCGTTACTGATGATGTTCCAGCTGCTGTACGCGCTTACAAGAAGGGGAAAAAGGAATACCGCGCTGACAGCTTTGGTAACATCCACATGTCCTTAGGCCGCGTGGACAGTGCGTCCAACCACTTGGTTGAAAACGCTTTGGCACTGCTTGATCTCATTAAATCACGTAAACCAGCAACCGTTAAAGGAATTTACATTAAGAACATTGCCTTGACCACTACCATGGGTCCGAGCTTAAAGGTTAAGCTCCCTGATTAATGGACAAGTTGCGCCTCGTTGTTGGTTTAGGTAATTTAGGTAAGCAGTACGCTGAAACCCGTCATAACGCGGGGTTTAAGGTAATTGATCGTTTACTAAGTCTTTACCATGTCCAGTTAGAGGAACGCAACAACTTAGGTGAATTCATCCTTTTAAGAAAACATAAGGTAGTGTTAGCTAAGCCAAACACTTACATGAACCACAGTGGTAAGTTTGTTAAATGAGCTTGTCAAAACTGAAACATTAAACCAGATAAGGTAATGGTGGTTTATGATGAATTAGCCTTCCCGTTAGGTACTGTTCGACTAAAAATGCAGGGTTCAGCCAACAATCATAATGGCATAAAGTCAGTAATTGCGCATTTAAATACTGAACACTTCAACCGCCTCCGGTTCGGAATTAAGTCCGATAATACTAGTAACATTCTCCATGAAGTGGTAATGTCGGAGTTTACCCCAGCCGAGCGTAATTTACTTGAAACAGCGCTTACAAAAGCGATTGAAGCACTCAAGGGTTACATTGATGGTGTGACAATGCTTAAATTAATGGAAGTTTTCAATGCCTAAAACACAATACATAGCTGGAGTATCAGGTGGTCCTGATTCAATGTTGTTGTTAAAACTGTACCACAAGAAGATTGCCTGTGTTGTACACGTTAACTACAATAAGCGGACTACTGCACTCCGCGACCAAAAAATGGTCGAAGAATACTGTAAACAGCTCAAAGTGCCCCTCATTGTGCACACGGTACCTGAAGATACCGTTTGAAAGAAAAACTTTCAAGCACAAGCTAGAAAAATTCGCTTTGAACAGTTTCAAAAAGCAGCTAGCCTTTATAAAGTAGATAAGCTGCTGTTAGCCCACCACCGCGATGATTTTATCGAACAGGCCAAAATGCAGTTGGACGCACGCAAGCGTGCCATGTATTATGGCATTAAAACACGTGGCGAATTGTATGGGATGAAGGTCTATCGTCCCTTTATTAAGTACTGAAAAAACGAGATCTTAGCCTTGTGTGAAGAACACAAAGTGCCTTATGGTATTGATGAAACCAACGCGCAGCCAATTTACAAACGTAATCAGGTACGCCAGGAGATCGCTAACTGATCGAAGGAAGAGAAGGAGGAGTTTTACATTGGTGTGTGCGCCATGAACCGCGTCATTGGTCAAAAGCTCTTTTCCTTAATGAAACGTGCAAAACAGTGGTTAGCTCATCCTGATGTACGTGAATTAAAGCGTTACCCACTTCCCGATCAACGTCAGTTGGTTTACAGCTTTTTAATTACCCACCACATTGATGTAACGGGGGACAAGATTGATGCCATTTTAGACTTTATCCAACCGTTTCAACAAAAGCATTACCGTCTCAAGGATGAGATCTTCTTATCCATTAAAGACGAACGGCTAACTTTACTGTACAAGAGTTAGATGAAAAAGTTATTAGTCAAGGAGTTAATCGAACAATTTCAGGATTGTGTTAACCTCATTGATGGGCACACTAACACCAGTAATGTAATTAGAGTACCCGGATTAAAGCGGGTGGTGTTTGAAATGCTGGGGTTGTTTTCCAGCCAAATTGGCTCGGTAGCCATTTTGGGCAAACGCGAGTTTGGCTTTTTGAGTCAAAAAACGCTGGTTGAACAACAGCAAATTCTCCACAACTTACTCAAACTCAACCCACCAGCGATTATTTTGACCAAGTCCTTTACTGACCCCACGGTACTTTTACAGGTTAACCAGACTTATCAGGTACCGATCCTAAAGACCGATTTCTTTTCCACGGAACTTTCCTTTACGGTCGAAACCTACATTAACGAACAGTTTGCTACTGTTGCACAAATTCACGGCGTCTTGCTGGAAGTTTTTGGTGTAGGTGTCTTATTAACTGGTCGCAGTGGCATTGGTAAATCAGAGTGTGCCTTAGATCTAATTAACAAGAACCACCTCTTTGTCGGTGATGATGCGATTGAAATATACCGTTTGGGTAATCGCTTGTTTGGTCGCGCGCAGGAAGTAGCGAAAAAGTTTATGGAAATCCGTGGTCTTGGTATTATTAACGTCGAGCGCTTTTACGGATTGCAAATTACTAAACAGCGCACCGAAATCCAGTTAATGGTGAACTTACTGAGTTTAGAAAAACAAACGACCGTGACCTTTGAGCGTTTAGGTACCGAATTGAAAAAACAACGTTTACTCGGAGTTGATTTGAGCTTTTATGAAATTCCGATTTCCCCCGGGCGGAAGACGAGTGAAATCATTGAAAGCGCTGTAATTGATTTTAAACTTAAGCACAGTGGCTATAACTCTGCTTTAGACTTTATTGAAAACCAGAAGGCCATTTTAAAACGTAAAAAAGATGAATCCTAGTGTTAGTAGCAGACCACCATGATCAACGGCCTTTTACTTAGGGCCGGGTTTTCCTATTCAATGGTATGGGATCATAGTCGCAATTGGGATTGCCTTTGGCATCTTAATGTTTGTCTTGAAGTTAATTTACTTTTACAAGATTCAAGATAACAGCTTTTACTTCTTTATCTTCATTGCAGTTTTAACAATGGTATTGGGCGCACGAGCTTGGTATTTTTTGATTGAAGCGGTTGATGGTCGTTCAAGTGGGAGCAACTTCTTTGACTTCCGCAACGGTGGGTTAGCAATCCAAGGTGGTGTGTTGTTAACTACGTTAGCGGGGATTATCTACTTTAACGTCTTTTTAAACATGAAGACGACCAAAACCAAAACCACCGCTAAACTATTAAACAACAAGAACCAGATTAAAACGGTTTATGTCGAGCGTAACATCTCAGTGTTTGTGATGTTGGATCTCATCGCTCCCTGTGTGTTAATTGGTCAAGCCATTGGTCGTTGGGGTAACTTCTTTAACGCGGAAGTTTATGGCGCAGCTTTAGTGGGTTCCAAAAATGATACCCTGTCAGCTGCTAACACCACCTGGGGCTTTTTAAGAATCTTAATGCCCAAAGTGTGGGACGGAATGTTTATCAATGGTAGCTTCCGCATTCCGCTTTTCTTAATCGAATCGTTCTTTAACACGATCTTCTTTGTCTTTATCTACTTTGTAATGGACCACATTAAGGGGATCCGCAGTGGGACAATTGGCTTTAGTTACTTCTTAGCGACTGGCATAGTTCGCTTAATCTTGGAAACGCAGCGTGATGAAGCGTTTAAGTACAATACCTCGATTGTGTTTAGTGCACTCTTAATTTTGGTGGGAATAGTAGGGATTATTTACTGTCAAACGTTAGCAATTAAATTGCGTGGTTATTTCTGAACTTACTTCTTCCTATACGGTTGGTACAAGGTAGCCGCTTTCTTTACTACCTTATTTATGAAGGATAGGACACAGGCTTGTTCTAGTAAGTTTGCATTTTACGAAAAGAGCTTACCTGAAAAGGAACGTTCCTTCTTTCAGTTGAAGTACTACAACGATGTTTTACCACCGAAAATTTACCGTTTGTACGACCATGAAATGCTCATGTTTGACAAGCTAGAAGCAGTGCCGGAAGCTTAGATTGTGTTACAATATTCGCGCTGTCTTTATGGCTACAATCGCACAATTAATTAGAAAACCGCGGAAAAAGAAGAAGGTTAAATCTAAATCACCCGCTTTACATTACAACCTCAATTTACTCAACAAAAAGGTAACCAACGTGTATTCACCACTTAAACGTGGTGTGTGTACCCGGGTTGGTACCATGACTCCAAAAAAACCGAACTCTGCTTTGCGCAAATATGCCAAGGTAAGACTTACTAACGGTTTTGAGGTGTTGGCTTATATCCCTGGTGAAGGACACAACCTCCAAGAACACAGTGTAACGCTACTCCGTGGCGGTCGGGTGAAAGACCTTCCTGGTGTGCGTTACCACATTGTTCGTGGTACCTTAGACACTGTTGGTGTGGAAAAACGCCGTCAACAACGTTCAGCTTACGGGGCTAAGAAACCAAAAGCCAAGTCCTAATATCTTAAATTCCTTTTTTTACGAAAAACATTAACTCAATCAATTAACTTTATATGCGAAAAAATCGTGCTCCCAAACGGACTGTTTTACCCGATCCCGTTTTTAACAATACGCTGGTAACCCGGATTATTAACGTCATCATGGAAGATGGCAAGAAGGGTTTAGCACAACGAATCTTATACGGTGCTTTTGATCTCATTGAACAACGCACTAAAGAAAAACCCTTAACCGTGTTTGAACGCGCTGTGGGCAACGTGATGCCGCGCTTGGAACTCCGGGTACGGCGGATTGCGGGATCCAACTATCAGGTACCTACCGAAGTACCTCAAGACCGCAAGATTGCTCTAGCACTCCGTTGAATTGCAATGTTTGCGCGTAAGCGTCATGAAAAAACGATGTTAGAGAAGATTGCTAACGAAATTATTGATGCATCGAATAATACTGGTGCAGCCATTAAAAAGAAGGACGATACGCATAAGATGGCTGAAGCCAACAAGGCCTTTGCCCACATGCGTTGGTAATAGAGAATAGAGTTAGTTATTTATGGCGCGTACAGTAGATTTAATTAACTTCCGTAATTTCGGAATTATGGCCCACATTGATGCGGGTAAAACCACCACCTCAGAGCGAATTTTATTTCACTCAGGCCGAATCCACAAGATTGGGGAAACCCATGACGGGGAGTCCGTGATGGACTGGATGGAGCAGGAAAAGGAACGCGGTATTACGATTACCTCCGCAGCTACTTCAGTTAGTTGGAAGAACTGCAGCTTAAACCTAATTGACACCCCCGGTCACGTTGACTTTACCGTAGAAGTAGAACGCAGTCTCCGTGTCCTTGATGGTGCCATTGCTGTGTTAGATGCTCAAATGGGGGTAGAACCACAAACCGAAACGGTGTGACGCCAAGCATCGCGTTACGAAGTGCCCCGGATTATCTTTGTCAATAAGATGGACAAAACGGGGGCTAACTTCGAGCGTTCTGTACAATCGATTCAACAGCGCTTAGGAGTAAAGGCGGTACCTATCCAATTACCAATTGGTGCGGAAAACGACTTTAACGGCATTATTGACCTGATTGAAGAGAAAGTTTACTTCTTTGATGGTGGTAAGGAAGAAAAGGCCGAGGAAAAACCAATTCCCGACCAGTTTAAGGATCAGGTCAAACAAATGCGTGCGCACCTAGTGGAAGAGGTGGCTAACTTTGACGATCAGTTAATGGCTGATTACCTTGAAGGTAAGGAAATTAGTATTGCCGACATCAAGCGCTGTATCCGCAAAGGGGTAATAGGTTGTCAGTTCTTCCCGGTGCTTTGTGGTTCTGCCTTTAAAAACAAGGGGATCAAACTGTTATTGGATGCTGTAGTAGATTTCTTACCTTCCCCAGTTGACGTACCGCAAGCCAAGGCTTATGGTGAAGATGGTAATGAGGTGTTAATCTCGGCTAGTGATGATGCACCGTTTGTTGGTTTAGCCTTTAAGGTAGCGACTGACCCATTTGTGGGACGTTTAACCTTTGTCCGCGTTTACTCCGGAGTACTTAAGTCCGGTTCTTACGTTAAGAATGTAAGAAAGAACAAAAAGGAACGGGTATCACGTTTGGTAAAAATGCACGCGCAGAACCGCAATGAAATCGAGGAAATTCGTGCTGGTGATATCTGTGCTATTATTGGATTAAAGGACACCACTACTGGAGAAACCCTAGTTGATGACAAGATTGATGTCCAACTAGAAGCGATGCAGTTCGCACAACCCGTGATCTCTTTAGCGGTGGAACCAAAAACCAAGGCAGATCAGGAAAAAATGTCGATTGCACTCTCGAAGTTAGCGGAAGAAGATCCGACCTTTAAGACCTTCACTGATCCCGAAACTGGGCAAACGATTATTGCGGGAATGGGTGAATTGCACCTAGACATCCTAGTGGATCGGATGCGCCGCGAGTTTAAGGTTGAAGTTAATGTGGGCGCACCACAAGTAAGCTTCCGTGAAACCTTCAATAAAGAAAGTGAAGTTGAGGGTAAATACATTAAACAATCGGGTGGCCGCGGTCAGTATGGTCATGTCAAGATTCGCTTTGAACCAAACAAGGACAAGGGCTTTGAGTTTGTCGACAAGATTGTTGGTGGTCGGATTCCGCGTGAGTACATTAAACCAGTGCAAGCGGGTTTGGAAAACGCTATGGCATCAGGTCCGCTAGCTGGTTATCCGATGATTGACATCAAAGCTACCTTGTTTGATGGTTCGTTCCACGAAGTTGATTCGAGTGAAATGGCCTTCAAGATTGCGGCATCCTTAGCTTTAAAAGAGGCGGGCAAGGTATGTAGTCCGGTACTGTTAGAGCCAATTATGGCAATTGAAGTGACGGTGCCAGAACAATACTTTGGGGACACCATGGGTGACATATCATCGAGAAGAGGTCTCATTGAGGGGACTGAACAACGTGATAACGTGCAGGTAATTAAGGCCAAAGTACCACTCAAAGAGATGTTTGGTTACGCCACTGATTTACGTTCCTTCTCTCAAGGTCGGGGTAACTATGTGATGCAGTTTAGCCATTACGCTGAAACCCCTAAGAGTGTGGTGAATGAGATAATTGCCACCAAGAAATAGCAAAATAGATAGGCATAAATCTTTACTTTTATATTTAGTTTTCCCATAAAGTTACGATGCAATACAACATTATTCTTTTAGTTGATGGTTCGCTCTCGTTAGAGCAAGCTAACCAAGTGAATGAAAAACAACAGCAAACGTTAACCAACGTTGAGGGTTTGCAAACAGAATACCTTGGTTTAAAGGAGTTGGCATACCCAATTAAGAAACAACTCTCAGCGCATTATTACCGCTGGAAGTTTAGTGGTGATAACCAATCCACGAAGGACTTTAAGCGTACCGCTAACATTAACAAGCAAGTATTACGTGAACTGATTATTAACTTAGAACGTGAGTACGGCTACTTAGCTTCGATAAATCCAAAAAAGCAACAGCTGGCACTGCAAAAACGCGCTAAGTATGACGAAATCATTGCGCGGGAAAACAACCCCGAAAACCCGGATGTACCAGTAACATCAGGTTTAGCTTCCACCCAACCACGCTTATCACGTACTGAAAAGGCACAAAAACCAAAGGAAGAGCTGTGGGATGTGGTGCAAAAGATGGGAAACTTTGACAGCGTTCAAGCCAACCCATACCGCCCACGTTTCAAACGCTTTAACGCGGAACACGTTAACCAACGGCAAAACCAACAAAACAATAACAATAACCGTTTTGACCGCAACCGCAACCGTCAGCACAACCGCTTTAAGGATAAGCAATAATGAACCGCGTTTTTTTATTTGGTAAGCTCTCGTTTGATCCTAATAAATTGCAAACCCGTACCAACAATATTGGCGCGTCCTTTTCCTTAGCGTGCATTGATTCGAGCGGTTTTAATGACAGTAAGTCCTATATTAGGATTACTGCTTGGGGTAAAGTTGCTAGCTTTGTTTTAACTTTAAAACCCGGTGACAGTGTTTTTGTGGAGGGGAGATTGTCCACTTACAAGATGAACAACCGCAGTGATGATCCCAATTCGAAGGCCACTTACGCGCTCCAAGTTATCGCTGATAAGGTTTACCGTCCCGATGAGGAAAACAGTTTAGAGCAACCTGTTGATAAGGCTACGGTAATTGATTCACCCTTTTTAGCAGCGAAAACCAATGCTACTGAAAATGAATTAGCCCAAGCATTTCCGATTAGTCTGGATGATGAAGATGATGACATTAACCCCATTTTAAACAACGATTCCCAACTAGAGGAGGAGAGTGATGATGAATAACGAACACGATAATTTCCAAAAAGAAGTTGAAACTACAACTGAAACAACCTTCAACAGAGAAGAAGGCAAAAGAATGGTACGTCCTTTATTTAAGCGTTCTAAAAAGTACTGCCGTTTTTGTGCCATTGGTCAACTTAGAATTGACTTAATTGATGATTTAGAAGCGTTAAAGCGTTTCTTGAGTCCCTACGCTAAGATTAACCCACGCCGGATTACTGGTAACTGCCAAATGCATCAGCGCCATGTAGCCAAGGCCTTAAAGCGTGCACGTTACTTAGCCTTAGTTCCGTTTGTAAAAGATTAGTTATGAAGGTAATTTTGAAGCAAGATGTTAGCAACCTAGGCAAACGCTTTGATGTGGTTGATGTTAAAGATGGCTATGCAATCCACTTTTTGTTTCCTAAAAAACTGGCTGCACCTTTAACGAAAAAGAGTTTACAAGACCGTGATTTGTTCTTAAAGAAACAACAAGAACACTATGAGATTAACAAGGCGTTAAGCCACAAGTTAAAGGAAGTAATTGAACAAACAGAACTCCACTTTAGCTTAAAGGAGCACAACGGTCGGCCGTATGGCTCGATTATTACCAAGCAAATTATTAACCAAGCCCACACTAAAGGGATGGCGCTGCAAAAGTTTATGTTTAAAGACAACGTGCGCTTAGGGTTTGGGGATCACGAGATTACCTTACACATCTTTGAAGATACAACCGCAGTTTTAAAAGTTAAGGTAACCCCGGATAATGGTGTCAAATAAGCTTGAACAGACCTTTAAGTTTGCCAACGACAAGAACATTGAACGTGCTGAGCGTGTTTTAATGCAAGCTTTAGTACAAGATGCCGTTGGTGTGGACTTAATCTTAACCAAGCTTGAACCGAAGGACTTCTTTGGTTTTCCGTTTAACTTTATCTTTCAAACAGCACAGGAAAACTATTCTGAGGGCAATAAGTTGTTTGGTTCGGCGCTTTTAGAGGCCGTTAAGTTTAAGCTGGATACCGATTCCAAAACACAAAGGGAAATTGAAGGTTTATTTGAGGACGTTTTACTCAAAGGTCTTCCCTTTTTGGAAAGGGACATCAAGGTCTTTGTTGATGTTGTCAAAAAAGCTTCGATTTTTCGCCAACTTAAACAGTTCGCCAAAAAGGTTGAAAAGGAAGAGTTTAAGGTCAAAGATGACCGTTTTGAGGGCTATTTACAAGCAATTCAAAACGACTTTACCAAGATTATTCACAGCGCCTTTGTTTCTATTCCCGGTTTGAGTTATGACGAAATTGGTCGTGAAGAGGAAGAGTTAATTCGCAAAGTGTACCGTGGTGAATTAACTGTAAAAGGGCTTAAAAGTGGTTTTTACCCTTTAGATCAATTAACCTCTGGTTGAAAAGAGGGTGAGTTAATTGTGGTAGCAGCGCGTCCAGGTCGCGGGAAAACAGCGCTTCTAATTAACTTTTTGCAGGGCGCGGTTAATGACAGCGAAAAGTTTGATAAAAACAAGGATGTGTTGCTGTTTTTTAGCTTAGAAATGCGTAACCGTGAAATTTATCAGCGCCATTTAATGCTAGAAAGCCAGGTAAATTACACACTCACGAGTCGTCAAAGGCTCGCTAATGTGTATGATGATTTAATTAAAGCATCAGAAGTATTGCGCGATCTTCCTATTAAAATTTTTGATTACAGCACGATTACGTTGGACGAAATTCGTGCTCAAATCACGGAAGTTTCAAAAACCAATAACGTTAAGTTGGTCGTAATTGACTATCTCCAACTAGTTAATGCATTTAAAAACAGCTATAACATTTCGCGTCAACAAGAGGTCACCATGATTTCCAAATCACTTAAAAGCTTTGCCAAGGAGTTTAACGTACCAATTATTGCCGCTGCACAGCTTTCTCGAAGAATAGAAGAACGCAAAGATTCGCGTCCCATTTTGTCGGATTTACGTGAATCGGGTTCGATTGAACAGGATGCTGACATGGTTTTATTCATCCACCGCGTCAAGGATGAGGACAGTGAAAATCAAGAAGCAATTGGTCACAACAATATTTTTCAAGTGGAGTTAATCTTGGAAAAAAACCGTAGTGGTCCGACTGGTAAAGTACAGTTTGACTTTCAAAGTGACGTTTCTACCTTTCGGGTTCGGGAATACAATCCTGATGGTTACAGTTAGTTTATGAAGTACAAAACAGTTAAATCGATCCCGCTTTTTTTGTTAGGCTCGATTGTGTTCACTGCTTGTTCAACACCACAATCAACCTTTCATTTACCTGTGCAAACGACCGTTAGCGCAATCAAAAAAGATATTAGTGGGAAAACAGCTACTGCTGTTAAGGCTGCTAGTAGTTCATCTAGTACTACTACATCTAACGATGACAACAACCAAAAGGGTTACTTCTTAGAAACTAATCGTTCCACCGGTACTTACGACCCTAACAATTCCACCCGGTTAATTAAATTAGGTGAAAGTGGTGATTTTCATGCAGCTGACCAGAATAAACCGGAAGAAGCCTTGTTTGAACGACTTTACGGCGGCATTGCTTCCTTATTAAATTTCCGCATTATTAAACCTGCTTTAACTTATTGAAACACAGTAACACCTTCTCTAAAAGCGATTGGTAAAAGCAGTAATCTAATTACCTTTAGTCAAGATATTGATGAAACGGAGTTACAACGGGCATTGGCTAATAACTTAATTGTCGCTGATGATGGCAACAACAACTTTTGGTTTGGTTTAAAGTCACTTAGCTTTAATGCAGCTAAGTTGACTGACAACGCCCAAACACAAATGGCGCAAAAAACCACTCAAGCAGTTACCTTGAAAAGTCAAGCGCAAATGTCAAGTACAAATACCAAAAACACCAACAAAAAGATTGATTTGCGCGATAAGATTACTTTATCGAGTACGATGAATACTCAAGGAAGTGGTGATAACAAAAACCCATCTAGTGGTCTTATTCAAAAGTTAGTGAGTGTCGAAAATATTGAAGCTGAATTTAGTTTTGTTAAAACTGGCTTCAACGGTAATGAAATTAAATTTGGCGATTTTGTAACTGAGAATTCACCCACAACCACTCAACTTAAGCAAGTTTGGAAAAAGAAGTGGGGCACGGAATTGAAAAAGACAAACTATAAGCTTCAATTGAACAACTTTAGTCTATTGCTAACTTACACACCAGAAGTTAATAAAGTTGAAAAAGGTAACAATGGTGATTCCAATAAAGGCACGATCGCTACGCCAAACGGTTTTAGCTTCTTGTACCCAGCTAATTTAAATGAGACACCAAGCAGTAGCAGTAGTTATTGAACTAATGTTACTGACTTAACTAAAGCAGCTACTGACACCGAAAATACCAATTTACTCAACGACTTGCAAAAGAGTCAGGAACAAGTCAATCAATTTGTTGCTGCTATTACCCAAAATCACCTCGATGTTTCCGAAGCTGCTTTAACTAAAAAACAGTTTGGTAGCCTTAGCATTAGTGATTTCTTTAAAGCCATCTTTAAGGAAAATGGCAAAGACACTAAAGCTAAAAGTTAAAATTAACCGTTAAATTTTCCTGTAGTTTTAGTTTTCCAGTAGTTTTTAGTATTTGATTTAGTTCTTCTAACTACTTTAATTGAAATTTCCAAAAAAGCGCTGACAAAAATGAGCGTTCCTGACCGGTAGTTTTACCGTTTTACCCACGCTAATTGCATTTTTCTCGATTTTACCGCACAGTTCTGTTTTAGAACCAAGCCACTTAAGGTTCAATTCAACTAGCCAGCGTTACCAATCTGACAACTCCCACCTCTTAAATTTACACGGTGAAGCATTTAGTGATGCTGACTTGAATGCAGTGGCAGAGCATACTGACTGAGACGCTAACTTTGCTTTATTTCAGCAAAAGTTCAAGACAAAGCCAATTACGGTTAACCAGTTAGGTTTAGTCGATGTTTTTAACTTGTTGAGTGGTTGAAAACAGAGTTTGACTGGTGTACTTGAAACAATTCCCCAACTGCAAAAGGAAATAAAAGCTGCTAATGACATTTTCCCTGTCAATGAACAAAGTCAAATTCCCCGTGTAGAAGCACGCTTATTTGACTTTTTGGGCAGTGGCTTTTTCCCGGTATTGAACCCGAAAGGTTTAGGGTTAGCGGACAACGTTGCTAGTTTGTTTGAACAGTTTCAGCTAGCATCAATAAACTTAAGTGGTTTTGAAGTCAAACTGATTAAAACGCATGACATTGTCATTAAGAGTAAGGTACGTTACAGCTTTGAAGTGCAAATGCAGTTCCATGCTGTTTACCAAAATAACGACACTAAAGCTAATTTAGATTTTGCGCTCAATATTAGTACGATTAACTTCGCTACCTTACAAGAATTACAAAACAGTTTTGATCTTCAAGGTAGTGATTTAACGGCTGGGTTGTTTTACAAATACAGCGTTAATAAGTTAACTAGTGGTACTAACGATTTAACCACAATTGCTAAAACCGCTTTAGGTGAAAACATTATCCAAAAGCAAGTGAGCCTAACACAATCAATTATTAAGCCACGCCTAGAAGCTGCAAAAACTCAGTACAAACAGGATATTATTGCACCTTTTGCAAAGGAACGTCAAGCTGCTTTGGCACAACATTTAAAAGAAATAGAAGAGGCCAAACAACGTGCCGAGCAATTATTAAAAGAACAGCAAGAAGCGGAAAAACGGCGCCAAGAAGAAGTTAAGAATGTCGCAGAAACACAACAGTTTAATGATAGCTTAACTTCAGCACAAAAATTTAAAGAGTACTGGTTAAAACAAGGTAAGGATGTTACCAAAAAGGTTGAACTCATTCAGGCGCTGAAATCATCTTTCTTCCGCAACCAAAACCGTACCTTTAACTTTTTAATTGCCGGTTTTCGAACTGCCATTGATTGGTACTACAATCAAGAGAAAAATAATACCACTGCTAAAAATAACGCTTTTGGTAAGAATGGCATTCAGTTCCCTGTGGCTGGCTTTCAGGGAATTTACATGTCACAGTGATTGCGTGACGAGCTCTCTGGTAAGACCGACATTAAGCTCAACTTAAAAAGTTTAAGTGTTCAGAATGAAAATAAAAATTCTTCCATTAATTGGAACAAGCAAAAAAGAATTGAGATTAAACAAGTAAAACCATTTAACTATTCTTTTGAAATTAACTTGAAGTACACTGGTAGCTATAATGTCAGTCTTTGATATCTAATTGGGGCTGCAATTGGTGGGATTCCAACATCTTGAAGTGGAACTATGGATATGAAGTTTATCGTTGATGGTGATTTGGATAGCGGTATTGTTACCAAGCAAGATTATCCCGGTTCTAAGTTTGAATTTACCGAAGACAAACTGTGGTTTACCCTGCACGTTAAACAACAAATTAAAGTTAAAGAGCAGGGCTTTATGAACTTGCTTAAGGGTCAAAGTTTGGACAACTTGGATCTACGCACTGGTACAACTAAACCGCCAGTAGTTGATTTAGCGAGCTACCTCCACTTTGTTATTTTGACAGCTAAGTAGTTCATTGAGCCGCAAAGGCAGCAAATGGAACAACTTTTAGACCAGTTTTTAAGTGGTGTTTTAAGCGAATGGAAGGCTTTTATTTTGCAAGAAGCCAAACAGACCTATTTTCAAGAATTGTTAGCTAAACTAAAAAATGTCGAGCAAGAGCTAACTCCCAAAGCCTCTCAAGTCTTTCGTCCCTTTAGCTTTTTTGCACCAAATAACACTAAACTAATTATTTACGGTCAGGATCCATATCCTAATCCACAACACGCTTGTGGTCTTTCGTTTGCCTCTAACGCCCCTAAACTACCACAAAGCTTAAAGCGGATGATTCTGCGCTTGCAGCAAGAGTATCCGGAACTAGCAGGTCAAAATCACTGAACGAAGCAACTGTTAGAGGGTTGAGCACAACAAGGCATCTTGTTGTTAAATGGCGTCTTTACCACCAATGCCTTTCAAACCAATGCGCACCGCAATTGGGGGTGAGAGCAGTTTAACTGTCATTTACTCGATTTTCTCTTGTCACAAAAACTCTATGTACTACTCGTTTTTTTGGGCAAACAAACCGAGAATTTTGTTCTTAAAAAGATTGGTGGAGCAAGCCAATTTGCTAGTTTATCCTACCCCCATCCCTCACCTTTAACCGGGCGGAAGTTTTTCGATCATCCCGATGCTTTGTTTAAGCAAATTAACCAGTGATTAAAGCACCATAACCATACTCCAATTGATTGGACTAACGGCCAGGTACAGTACCAGTTCTAACTGTTTTGGTAAAGTTTTGTCTTTAATTCAACAAACAAAGCTTTAGCATTTTTCATTATTTTTCTTATGATTTTTATTCCGCTTGTACTCAAACAGAAAAGCACAGCGAGCAGTCTTTTCTTTACAATGTAAAGAACTGCAAAGCTGCTTAAACTGTCTTTGCAAGCTTGTTTTAGAGTTGCAAAGCAAAGTCCAAAATGGATAAATTAATAAATAAACCTCAACCATTAACTTCTGATATTTCCACTTCTGGATTTATTTACTTTGCGGTTGTTTTTGTAGCCATTATGGGCTACCTTTTATTCAAAAATCTACTGTTTTTATTTTTCTTTAAACGCTATCCAAAAAACACTCCCAAAATTGGGGTAGGTAACATTACCACGATTGCCATGATCATTGCTGTGGCGGTGTCGATTGTTTTAGTGTTAATGGCACTAGCAGGCGGTTTAGCCGCTGCTTTGTTCCGTGGTTATCCTGGTTTTCGGGTCACCTTGGAGTTAATCCTTGTAAAGATTTCGGGACTGTTGTTTGGTCCAATTGTCGGCATTTTTTCCGCGGCCACAATTGACTTTTTAACGGTCATCTTTTCGGGTGGGGTTTTTAACGTTGGTTATGTTTTAGGTGCCATCTTAACCGGGATGATTGCTGGCATACTGCGTGAAGTTTTAATTTCAACAGCACTTTTACACAACCGTAATTTATCGGATTTTGCCTACCTAGTGCTCTCGATTGGCATGGTCATTGCCGCCTTTTTAATTACCCAGTTCTTTGTGCTTGGGATAAGTAATAACCTTAAGGAGATTAAGGGTGATGAAGAGTTTCGCTTGAAATTTAACGCACCATCAATTGTCTTTGAGCTTTCGTTAACCCAGTACGCCAACATTCTGCTCTACTTTACCATTGCCATAGTTATTGCTATGCTGGTGTTGTACATTGTTTGGTTAGTCAAACAGCGTCACTTAAGTTTTGAGCACTCTCGTTTCTTTTACCGCAGTTACAAACATGCTAATCACCAGTTTACCCTGTTTGTACTAACTAAGGAAAACTGGTTCTATTTAATCTTGAATGTAATCACCTTAGCTTCCACTAGTTTGTTGATGATCAACATTGCCTTCATTCCGATCTTTGACACGCAAACAACTGGTCAAACATACGAGTTTTGACTATTAGCACGCTTACTGTTCGCTCCCGTAATCTTCTTGTTGGACATTATTGTGATCTATCCAATTCTGCTGTTATTAACCCCGCTAATGCTCAAAGGTTTTAAAACAGCAGTTTCTAAAAACCAACGCAAAACACTAAAACAAAGCTTTACTGATTTGCAAAGTGTAGTGTTGCCCATTATTAACAAACGTAAACACCAGCAGTTGCGCCAAGAAGAGTTAAAACGCTTAGCACGAGCCACTCACTTTGATTTGACAGAAGGCGAAATGGAAAAGCTCTTGGTCGAATTTAAAACTATTACCCAGAGCTTTGACCGGGTGATGAACATTGATACCACCAGTGTGGAACCAATGTACGCACCTTTCAATACATCACCAACGCCACTGCGTAAAGATAAGGTAATAGTGGAAAAGCATCCCGAAAAATTATTAGCCAACTGTAAGGAAATGAGTGTTGGCTTTGTCAAAGTATAGTTGTGAAATCGCAAATCTTAAAGCTCCAGCAAACACTAACTAAAAAACCAGCGTCAATCAATCCGTTATTACAACAAATTGATGGAGCAATTAACGAACACTGATCGAGTAACTTTTTGTTGAAAAATACGGTTGAGTGAGCACAAGCCCAAGCACCCAAAAACCGCAGTAAATCACCACTCAACAACATTCCCTTTGTTTTAAAGGACAACATTGCCACGAAGGGAATTGTTACTACTGGTGGTTCGAGGTTTTTGGAAGACTATATCCCGCCGTTTTCAGCAACTGTGTTTGAATTGCTAAACAACAGTGGCGCTTTATTAGTTGGTAAAGCGAACCTCGACGAGTTTGGTTTAGGCGGTACGGGGCTCCATTCGGGTTTTGGTTTTGTCCACCACCCTTGAAACGAAACCCTAATTCCTGGTGGTTCTTCATCCGGTTCCGCTTATGCAGTGGCGCGCGGCATTGTTCCCTTTTCAATTGGTACGGACACGGGAGATTCGGTACGCCGTCCAGCTAGCATTTGTAACATTGTTGGTTTTAAGCCCACCTATGGTTTGATATCGCGGAACGGAGTGTATCCTTACGCGCCTAGTTTAGACCATGTCGGGATCTTTGCGCGGTATGTGTATGATGTAGCACTTGTTAGTGATGAAATCATTAAACATGACAAAGCTGATTTTTCCGCTCAAAAATCTCCTGATGCTGGCAAGTTTACCAGGTCCTTAAAAGAGTCCTTCAACAAGCAAATCAAGATAGGTTATCTAAAACCGCTAGAGGAATGGTTTGACATTGAACTGAGCAAAAAGTGAAACAGCTTAAAGGAAAGAATTACCTTGGAGGGTTGTGAGTTAATTCCGTTTCACTTTCCGCTTGAACTGCTAGAGGTAATTGATCCAGTTTACAAGCTGATTAGTTACAGTGAAGCGGTGAGCTGTTACAGTAATCTCACTGGGATTGTCTTTGGTCAAAAATTGTTTGAACCCAACCAAGCGAGTGACTTTTCCAAAACAATTACTGCCAACCGCGACCGTTTCTTTGGTGAGCAGTTAAAACGCCGCTTTATTATTGGTGCTTTTGGTACTGACAAGAATAACTTTACCAAGTACTTTGAAAAGGCTCAAAAGATCCGCCGGGTAATGGTTGATGCTTACTTAAACCTGTTTAAAGAAGCGGATTTCATTGTGTCACCTAGTGCATCCGGCTTTACTAAAACAATAGCTGCGGTGCAAAAGGGTGAGAGCTTTACTAACCTCGTGGATGACTTTTTACAACTAGCTAACTTTGCTGGTAATCCTTCAATTACGATTCCATGATTGGTCAAACAAAAGGACCAAACGATTGGTTTGAGTGTTAATGCTAACTGCTTTCACGACAAGCAATTATTACAAGTAGCTGCTTGATTGGAGGAATTATTCCAAATTGAACATGATGATTAACTTTGAAGCAATTATTGGGATTGAGGTACACGTGGTCTTAAACACTGCTACCAAAATGTTTTCCCCAGCGCCCAACCAAGCGCAAAACGCCACACCAAACCAGTTCATAAACGCGATTGATTTGGGCTTGCCTGGTACCATGCCCCAGGTCAATGAGCAGGCGGTACAAAAGGCGTTAATCCTTGCTGACGCGCTCAACATGCAACGGGTACAGCCAGTCTTAGTGTTTGACCGTAAGCACTACTTTTACCAAGATCTACCTAAGGGTTTTCAGATTACCCAGCAAAACTTTCCGATTGCGCAAAACGGTTACGTTGAAATTGTGGAAAACAACAAAGCGCAGCGGATTATCATTGAACGGTTTCACCTAGAGGAAGATACTGCCAAGCAACATTTTGTCGATGGGCAAATTCTGCTCGACTTTAACCGTTGTGGTGCACCGCTAATTGAAGTAGTTACCGCGCCCGTGATTAAGAGTGCTAAGCAGTCTAAGGCATACTTGCAAAAGCTACGTCAAATTTTGATTGTTAACAACATTTCCAACGCTAAGCTCGAAGATGGCTCGATGCGCAGTGACTGTAATGTATCAGTGCGCTTAAAGGGACAAACTGCTTTTGGCACCAAGGTCGAAATTAAGAACATTAACTCACTCAATAATGTGGAAAAGGCAATTGATTTGGAAATAGCACGTCAAGTCAAAGCACTAATTAAAGGCGAACCGGTTCAACAAGTTACCCTAACTTATGACGACAAAACCAATACCAATGTCTTCATGCGTAAAAAGGACAATTCAGTCGATTACCGTTATTTCATTGAACCCAACATTATGAGCAGTAACATTGATGAACTGCTACAAAAACCCAACAAGGCCTTTAACTTAACTGAGTTCTTTACCAAGCTCAAGGAAGCTGGGGTTAATGAGCAGCTAAATCAATTAGTAGTCGATGATCTGACTCTTTTTAATGCTTACACCAAGATTAACAGTGTCATTAACAGTCCCCAAGATACCATTCGCTGGTTGTGCATTGAGTTAATGGGACAACTCAACAAGCTACAAAAACCGCTCAAGGACAAAACGATTGATCACTTAATTATCCTAATCCAAATGGTACAGAAGGGCACCGTGAACCAAAAACAAGCAAAGCAGTTAATTGAGTTAATGTTAGACAATGGGCAAGACCCCCAATCGCTGGCTAAACTACACAACTTAGAACAGATAACTGATGAAAAACAACTAACGCAAATCATCCAACAAATCTTTAAGGAAAACGAGGGTGAAATCCTTAAGAACTTAGATCGAGTTGAACGAATTCAAAAGCTAATTATAGGTCAAGTTATGCAAAGAACCCACAATCGCGCCAATCCCCAGCAAGTTTTTATAATTGTTGAAAAGTTGCTTCATGATTTCAGCGAAAGAGCAAGCTAAAAAAGGTCAAATAATTTACCGTTACATTCTCCTTAAAATTCAAAGCTTTAAATGGCCTGCTGGCACCCGCATCTTCTCTGAACGCCAGCTCGAAATTCGTTTCGCTTCATCTAGAAGTCTCATTCGTACTATCCTCAATACCCTCTTAGGTAAAGATCTAATTCGCCACACTAAAGGTAATGCTGGTTACTTTGTCGCTAAGAATGCTGGTTTTAGTTTCTTTCATAAAACCCAGGACAACAAATTGCCCAAGTGAGCTAAGCTCTCAACCTTAATGAAGAACCACCTACGCGAAGTTGACCGTGATGTTTTCAGCTCAATTGACAGTGGCGTTGACTTTGGTAACTTTAAGGGTTTGGAAGCCAAGTTATTTGATGAAAACAAGAAGAATTTTTTAAACCTTAGCTTCTTTGGCAAGGATGATGTGCTCCAAATTTTTAGCGAGCAAAACCTCCAGGAACAATTCTTTAAGGACTTTGCTTACAACGGCATTGTGGTAGAGCGCAAAAGCTCCTTAATCTGTGTGGATGATGAGACTAAGAACTTACTGATCTACGACCTCTTTTATGATGACAACAATAAGTTCATAGTAGCAATGCGCTCTGAATATTTAAACCCCCGCATCAAAATTATTAATGCTTAAAGTAAAGTCAGATTTTTTAACTAAAGATCAGGTTATTTACGATGTAGCGATAGTGGGTGCTGGGCCAGCTGGCATAGCAGCGGGTATTTATGGCAAACGCGCTAACCTCAATCTAGCCATTATTGAAGGTAGTACTCCCGGCGGTAAGGTTGTCAAAACCAACATTGTCGAGAACTATCCGGGTTACAAGTCAATTACCGGACCTGACTTGGGGTTGGAAATGTACAACCACCTAATTGATTTAGAGCCCACCTTTTTCTATGCTAACCTAATTAAGTTAGATAAGGCAGCAGATACCTTTATCTTGTACCTCGATGACAAAACTGTGGTGTTTGCCAAAACCGTTATTTACGCTACGGGAATGTTGGAGCGTAAACTGGGCGTAGCCAAAGAAGACCACTTTTACGGTAAGGGAATTAGTTACTGTGCCATTTGTGATGGGTCCTTGTACAAAGACCAGGTGGTGGGTGTTGTTGGTGGGGGGAACTCTGCCATTCAGGAAGCGTTGTATTTAGCTAGCATGGCTAAAACGGTGCACTTAATCCACCGCCGTGAGGGCTTTCGTGCTGATGAAACGGCGTTAAATAAACTGAGAAACTTACCGAATGTTGTTTTTCACTTGAACTATACCGTAAAAGAATTGTTGGGTAACAATACCCTAAACGGCATAGTGCTTCAAAACACGCTCGATCATTCAACAAAACAGATAGATCTCAATTGTGTCTTTCCCTATATTGGTTTTGAAAGTATTACCAAACCGGTGGAACACCTCAACTTAAAGTTAGATCCCCAGGGTTTTTTAATTACCAATGAACAGATGGAAACAAGTTTAAAGGGTCTGTTTGCAGCAGGTGATTGTCGTTCTAAGCACTTCCGTCAAATTGGTACTGCCATTAATGACGGCATTATCGCGGTGTTAACGATCCGTGATGTCCTTTAGTGTTCAGATTAAGCACGAGTTAGCCACCAACCCTTTAATTAAAACAGAGTGATCCAGCTTTTTAGCTGGTTATTTTCAAAACGGGTTAAAACTACTCGCTACTGGTCAATGATCCTTTAAAACTAGCACTGCTACTTTAAAGGCGTTGTTTAGCAATGCACTCCAGTTTAGTTTTCAAATCCACGAAACGAGCACTCACTGTGAGTTTAGTTTTAGGGCTTCGCAAACAGAGGTGGATCAGTTGTTAGCCTTTGATGCAACTCAGAGCGATTTACCGCTACAGAAGGCCTATGTCATTGGTGCCTTTTTGAGTGGTGGTAGTGTGAGCGATTTACTCCATTCCAGTAACTTTCACCTCCAAATTAGCACTAACAACGAACTGCAAATAAAACAGTTAATGAAGCTGTTTCATCCTTTTAAACAAACCACTAAGCGCCACCAGCTTTTGGTCTATGTTAAAAGCTATCAGGCCATTTGTGACTTCTTTAAACTAGTCGAAGCTTTTGATGGTTATCTAGCCTTTGAAGAAAACCAATTGCAAAAAAATTTTGCCTTAGAACAAGTGCGTAAAAGTAACTTAGAAATTGCTAACCTAATGCGCACTTTAAAGACCAGCACAAGCACAGCGGAACAGCTAAAAATCCTCATAAACAGTGCTGACTTTAAAAAGCAATCGCTCAACTTTCAGCGCTTTTGTTTAGTCAAACTAGACCATCCTGACTGGTCATTGGAACAAATTGCCCAGTTTTTTGAAAGGAAATACAAAGTGCAAATTACCCGTTCGGGAATTCAACACCTCAACGCTAAGTTAAAAAAACTAAACCAAAATTAAAGCATTAGTTTAAAATTAACACACATTAAAGCATGGACGCAATTCAAATAGTAATGTTTGTAATGGCAATCCTTTGTTTAATCATCGGTTTATTGCTATCCAACCATGGTTCTACTGGCGGACTTGCTTCGCTTTCAGGTCAAGATTTGGAAATTTTTCGCAAAACCAAAGACCGCGGAATTGTCAAAATTCTCCAAATTACGATGTTTATTTTAGTAGTGCTTTTTCTTATTTTAGGGTTAGTTTTTCATTTTGCGCTTTAAAATCTAACAGATGAAAGTATTAACTGATTTACAAAAGCGAATCTTCGCGATTGTGAAAAAAGAGAACGGCAAACCTATTCCTCCGGGAATAGTTGTCAGGATGATGGAGAATCAAGCTGGTTTTCCGGGAAAGCAACAGGTATACAGAGCAATTGATGACTTGTTAGAATGACACATTTTTCGCAAGTCGGGTGGTGCTACAAACCAGCTGTTAATTAACTATGAACTAGCTGATCCGGTATTGGATCAAAAGTTTCAGGGCATTCTCAATTTAGGTAACAAGAACACTGGTTTTGTCCGTCCCTTAGATGATGACAAAACGGTGTACTATATCCATTTTTCCAACCTAGCGGGAGCACTGGATGGTGATCTAGTTGAATTCTGTCCGCTCGACAAACCACAGGTAGGTGATAAGTTTGATGCTGCAGTGCTCAAAATTGTGAAGCGCTCCCGGGTGCTGTACGCTGGTAACTTTTTGATTGAGTACTCCGATTTTGGTCAGGAGTTTCGCATTGTTGCTGACAATCCTCGTTTTTACCTCACCCCGATTGTCAATAAGGCCTCAGTTCCAGCTGAACTGGAGAGCAATACTAAGGTCGCTTTTCAAATTGATGAGTATGATCCTGCAAATAACCTGTGTAAAGTCTCCATTCAGCAAATCTTGGGGAATAACGATGAACCGTTAATTAACCTCAAGGCGATTATGTTAGACCACTCGATTGTCTTTGAAGATAATGATGTGGTCGAACAACAAGCAGCGAAGTTACAGTTTGATGAAAAAGAGCAGAGTAAGCCCTACCGCAAGGACTTAACCGAACTTGCCTTTGTCACGATTGACCCCGCCACTTCCAAGGACTTGGATGATGCGATTTACGTCAAGCGCACCGACAAAGGTTTTGTTTTGTATGTTGCCATTGCTGATGTGGCTTACTATGTGCAGCGCAACAGTGAACTTGACATTGAAGCCCGTCATAAAACAAGCTCAATTTACCTCCCGGGATACTATGTGGTACCGATGTTACCAGAACGTTTATCAAACGAGCTGTGTTCTTTAAACCCAAATGAAAAGCGGTATGTGGTGGTGTGTGAGTTGAACTTTGATCATGAAGCACGCTTAAACTTTAGTGAAGTGTATCCCGCTACCATTGTCTCCCAACGTCGCTTTGCTTACAGTGAAGTTAATGATTGACTGGAAGACAGTGATGCTCTAAAGGATGAAAGTGCAACAGTACTAGAGAGCTTAAAGGCTGGTTTTACCTTGAGCGAATTGATTGCTGAACAGCGCAAAAAGAAGGGGACTATTGACCTATCTCACAGTGAAACAGAAGTAGTCGTTGATCAAAACTACTACCCGATTGAGATCCGTTTTCTCACCCACGGCAAGGCGGAAACAATGATCGAAAACCTAATGGTGGTTGCCAATGAAGCGGTGGCTTGAACACTTACAAACCACAAAGTTCACTTACCTTACCGGGTGCATCCCCGACCAAGCAAAAAGAAGTTACAAATGTTGTTGGAAAACATTGTCGAGCTCAAAATTACCCAACCAAACTTTGTACTCGATACGGTCACTTCCACCCAAATTGCGGCTTGGTTAAAGGAAAATAAGGACAACCCGAGCTATGACATCTTTGTCATCTTGTTATTAAGAACGCTAGGTAAAGCCTTTTACATTGTTAATCCACTGATACACTTTAGCATTGGTTCACACCACTATACCCATTTCACTTCGCCCATCCGTCGCTATGCTGATTTAACGGTACACCGTTTGTTGTGGATGAATCTGTTTACTCCAGAGCGTTTTACTGACACTGAGCGTGAACAGCTCAACGCCGAGCTAGAACAAATTTGTGAAACCATTAACGATACTGAGATTAAGATTAATGGTTGTGAACGGACTGCCAACGATTACTTAACTACGCTGTATTTGTCTAAACAAGTGGGACAAACTTTCCACGGTTTTATTTCCGCTATTACTAGTTTTGGCATCTTTATGCGAATGGATGAAAACAACTTTGATGGACTTATTAAGATTACCTCCATTCCCGAAGACTTCTTTGTCTTTGAAAAGGATCGGATGGTGTTGCGCGGCAAACGCACCAACAAGGTTTTTAGAATTGGTGATAGGTTAACCGCGAAACTGACTGAAATTGATACAGTGCAGAAACGCGCCATTCTAACCCTGGTTTAATGATGACAGTGGAAGTCTTTTCGGTTATTATCCTTGTCCTTTTATTCTTAATCTTAGCGCTTACCTTGTTGTTTGTTTTACTCAACAAGCGTACTAGAAGCTTTGTTATTCGTACCTTTACTGGATTATTTAGATCAAAGCATACTACCAGTCAAAAGAACTTTTATGACAACTTGACCTCAACCTTACTGAGGTTATCAACTGATAAGATTGGTGCCATTATTGCCATTGAAAACCAGGACTCACTCGAGTCTTACGTCAATATTGGCTACCGCGTGACTTCGGACTTTTCCCCCGAGTTGTTAGTAACGATCTTTTACAACAAGCAGTCGCCACTTCACGATGGAGCGGTAATTGTGCGGGACTACCAGATTGTGAGTGTGTCCTCTTACTTCCCAATGACACGGCAACTAATTGATGTGTCATACGGTTCCAGACACCGCAGCGCGTTAGGTCTAACCGAAAAGTGTGATGCCATAGTCTTTATTGTTAGTGAAACAACAGGCAAAATTTCGGTGGCTGTGCGCGGCGTGATTAAAACACTGTCGAGTAACTCCGACCGTTTACAAGACCAGATTATTCACTATCTGACGGTAAAGCCGGGTTAATTACCTTCCACTCTTTATTTGTAAACAAAGGGTTTAGCGGATTAATGCGGTGGTAGTAAAACCGCCCTTGCAAATGATCGAATTCGTGTTGCAAGCACATCCCAAATAAACCAGTAGCAGTAATGGTGATCTCTTTTTGTTGGAGTCAATCAAAACCAGTGATCGTAATCCATTCGTGGCGAATAACATAACCCTGGTGCATTTTGGGAACACTCAAACAACCCTCACCGCTCTTTAAAAAGGATTTGTTGGCACTTAAGTTGATAATCTTGGGATTAATTAACAGACACTTGTGTTCCACCCCACCATCCATTAGGTGGATGTAGAACATTTGCTTTCAATAACCAATTTGGTTAGCGGCAATCCCAATGCCGGGAATAATGCCATACTTCTCAGCATCACCGTTGTAAGATGCATCAACGTAAGCCATCATTTTGGCAATGCAATCGAGACTCGCTTGGTCTAAGGGAAACTGAACTGGTTTAGTCGGTTCGTTAATTTCCTTAACATCGTCCAGCACTAGTCATGCTTTAGTTGGCAATAATTCCATATTAATTTGCGGTAAAATTAAAATTATTCTAAATATTGAAATGGTCGATACAGGGAGAATTTTTGTCATTACCGGACCGAGCGGTGTTGGCAAAAGTAGCCTTGTTAGATGCTTAATTGACCATTTCAAAGATAAACTGCGCTACAGCATTTCCGCTACCACCCGTAAAATGCGTAACAGCGAAACTGAGGGTGTGGATTACTTCTTTAAAGATAAAGCGGAGTTTGAAAAACTGATTGCTGCTGATGCCTTTGTGGAGTGAGCGATGTATAACGATAACTACTATGGTACCCTTAAATCCCAAGCTGAACAAATTATTCACAACGGTGGCAACTTGGTGTTAGAAATTGAATATCAAGGTGCTTTACAAGTTAAGCAAAAGTATCCCAATGATGTGGTGCTAATTTTTATTAAACCACCTTCAATGGAAGAGTTGTTAGTGCGCTTAAAGAAGCGTAATGACGAGGATGCAATAACAATTCAAAACCGATTGAAACAAGCTGAGAAGGAATGTCAACAAATTGGTCACTTTAAGTATGTGGTTACCAACAACGAGTTTGACAAGACCTTAGCGGAGTTACAAGCAATTTTACTAGCTGAATTTAATTAATGGACAGCACCAACCAAAACCTGTTCGCGTCATTGTCCAAAAAAGGACCCGTTCGCAAAGAAAATCAGGACTTTAGTGTTGTTACTTTCAACCGCTTTGGTCAGTTAATGAGCCTTGTTTGTGATGGTTTAGGCGGTTATAAGGGCGGTAAGATGGCTAGTGCCTTGGTGAGTGAAGTTTTTACCAAGTCCTTTACAGTCTTTGATTTTCATAGTCAAACTGAAAGGGCAGTGAAACAGTGGTTTGAAATTACTTTAATTGAAGCGCGGCGCACCTTAGAACAGTACTTTCAAACAATTAAACGTAACCAAGTCCAGTTTGCGCGAATGGCGACTACCTTAGTGTTGAGTATTATAAGTAAGCAAAACATTTGAACCTTTTGGGTGGGTGATTCACGTGCTTATTTAATTAACAGTTACCAAAGCTTGCAAATTACCGAAGACCATAACCTGTACAACCAACTGTTACAGATGCACGCTACCCCTGATGTGATTGCCTCTTATAAAGACAAGCTGTTAGCCCTCACTGCTACCGTTTCCAAAGATCAGGAACGCCAGTTGAAATACAGCTTTAGGTGTGATGTAGTAAACGCTTGGGACTTTTTACTGTTGTGCTCTGATGGTCTGTACAACTTCTTGGATCCCAACTGCTTTTATGAGGTCATTACTAGTGCACCTAACCTCAAAAAGGCCGTTACACAACTAGCAAAGTTGAGCTTGGATAACGCTAGTAATGACAACATTACGCTCAACTTGATTAATTTAAAACAATGGCACTAAATTTAAAGATTGGTGACATTGTCCAAAACAAGTACCGGATTGAAAAGCTAATCAACCGTGGCGGGATGAACTCTTACCTGTTTTTAGCGAGCAACCTCCACGTCCAGGAATTTGGTCCACTGCAAAAGCGCCAGTTTACCCGGTTGGTGTTAAAGGTAGTACAACGCACCGACAAGATTAACGACAACAACTGGAAGAAGTTTTTGGATGGAACCATTACCACGACACGGGTGTCACACAAAAATCTGGTGCAAACCTTTGATGTAGTTAGCCCCCGTTTAAGCGTTTTGAGTGAAAACCAGGTGATCGTTTTAGAGGACACGGTGATGATTGTAATGGAGTATGTTGATGGTCCTTCTTTGCGTGAAATGCTCAACCAGAAGGGTTACTTTAGTGTGCAGGAGGTGGTGTACTACTTTACAAAGCTAGTCAAAGTAATTAACTACCTCCACAGTTTTGAACACCAAATTATTCACCGTGATCTAAAGCCAGAAAACATCCTGTTTACGAGTAACTTGACTGACATTAAACTGCTTGACTTTGGCATTGCTTCCGCTGTTATTCGTAACGCTGAGAAAACTGAAGTGTTAACGGACGAAAACTCGTTGTTTGGTACAGTTAGTTATATGACACCCGAAGTGTTGGAAAGTACTGTCAACAAAGAAGGCAAACGGATTCGTAAACCCCCTACAGTGCAGTATGACATCTATTCATTAGGAGTTATTTTGTTTGAAATGTTAGTCGGACGCGTACCGTTCAATAAATCGATTGATCCTAAAAAAGAACGGGAAACCATCCAAAAGGCGCGTAACTTTGATGTACCTTTAATGGGAAACTTGCGCAGTGATGTTCCGGTTAGTTTGGAAAACATTGTCTTTAAATGTACGGCTGTTAAACGTGAAAACAGTAAGTGGATGTATAGTGACACGAAGCAACTCTTGGCTGATTTGGCGCAATGACAAACCGAGCAAACCTTAATTAAACCAGTGCACGAACGTATTCTAGAAGGACAAAACGAAATGCGTGAACTAATGGTAAGTAATTACCTACCGTGGTATTTAAGGAAGGGTGTTTTGATCTTTTTTTCTGTTGTTTTATTAGCACTCTTAATTGCTGTAGTTAGCTTTTTTATTATTACTGGGGTAGTTGTCCATTCGTAAAACTGGCATAGTATTTCAACGCTTTGGAAAGCAGTTTAAGGTGTTTGTAGAAAACCAAACACTAGTTGCTTTTGCACAGAAAAAACTACAGTGAAAGCGCGATTTTAAACTGTTAGTTGGGGACAAAGTTACCCTTGAGAATGGTGTCATTGTCGCAGTTGAAGAGCGTAAAAACACACTGGTGCGCCCAAAGGTAGTTAATGTAGATCAGGTAGTAATAGTCCAATCACTAATTGAACCCAAGATTAACTGACAACAGTTGTTTAAATTGTTAATCCATTTTCACGCTCAAAACGTGGCACGGTTAGTGTTAGTCATTACTAAAAACGACCTTGAGTTTGAACCTGCTGAAAAAGCCCGTTTAAGTGAACTAACCAGCTTTGGTTACCAACTCTTTTTTACAGCCCCTAATGCTGATTTACCTCTCACTTTATTTACAGAATTGCAAAACCGTTTTAGTGTATTCATGGGACAATCAGGGGTCGGTAAGTCTAGCCTTATTAACCGTTTGGACAGTCAAATTCACCAAGCGATCCAAGCGCTTTCAGCACACCAGTTCGGTAAAAACACCACCACTTCCACGGTGATGTTTCCCTTCCAAAACGGCTTTATTTGTGACACCCCTGGCTTTAATGTAATTGACTTTCCCAACCTCAAACAATTAGCAGCCCAACACTTTGTCGGTTTTGCTAGCTTAATTGGTCAATGCCATTTTTCCAACTGCACCCATCAAAGCGAAAAAGGTTGTTTTATTGTAAGTGCTGTAACCCAAAAATCGTATTCCTTGTGACTTTATGAGAGCTATCTAAAGTTGATTAATTAAAATCAACTAAAAAAAGGATTTATGGAAAGTAAATGGTTAACAGTTGACACTAAGCACCTCTATGGTTTTGATGAAGCGATTTTTATTCAGAAATACCAAAAAAAAGTTAACCAAATTCACCAACAGTTTTTAAATCAACAACTACCCGATGGTCACATGAATGGCTGGTATAGTCAACCCGATCAAGACCACAAGGGTTTGCTAAAACAAATTAACACAATTGCCAAACAGTTTAATGCTCTAAAAGTAACTGACATAGTGTATTTAGGTATTGGTGGTTCTTATACTGGCATTCGTGCCATCTTAGACTTTTTAAAACCAGAACAGAAGGCGAATATCAAAGTGCACTTTGTTCCTGACATATCGGCCTTTAACATTGCTGCAGTTGCTAGAGCAATTAAGGGTAAATCATGAGCTTTGGTAGTAACTTCTAAATCTGGTCGCACACTCGAGCCGGCTGTTACCTTTCGGTACTTCCGTAATTTATTGCACAAACAATACAAGCAAAAACATGCTTTACGCACAGTTGTGATTACCGATGCCGTTAAGGGTTTACTAGTTGGCATGAGTAACCAGTACGGTTATGCACACTTAACTATTCCAAGCAATATTGGTGGGCGTTTTTCAACTCTTTCACCCGCCGGTTTACTGTTAGCTAAACTTTGTGGTCATGATCCGAAACAGCTCTTATTGGGTACTTTGACAGCCAAACAAGAGTTAGCAAACAGTGATTTAAACACTAATTCGGCTTATTATTATGCTGCTTTGCGTCACTGACTTTACACGACAAAGAAGCTCAAAATAGAAGTGACGGTAGCATACCACAGTGCTTATGAGTACCTCTTGTTGCAACACCGCCAACTGTTCGGTGAATCAGAGGGTAAAGGTGGTAAGTCCCTGTTCCCTACCTTTTCGCTATTTACCACTGACTTACACTCAATGGGGCAGTTGTACCAAGAAGGGGAAAAGAACTTCTTTGAAACAGTGATACAAGTGCAAACACAATTTCACGACTTGGAACTACCACCATCAGACTTTAACAATGATGATCAGCTAGATTACTTATTGGCTAAAAGTATGAATGAAATTTCAAACACTGCCCTAGAAGCAGTTGTGGAAGCTCATTTTCAATCAAACGTTAACATCATTAAGTTAACGCTCAAAGAAAGAACAACCTTTATGTTTGGTTACTTTTACTTCTGGTTATCAATGGCCACAATGATGAGTGGTTCACTGCTGGGACACAACGTCTTTGATCAACCAGGGGTGGAAGTGTACAAGCAGTTAATGTTTGCTAAACTTGGTCGTGAATAGGGAAATTGCCTTTTCGCTTTTACCACTGTTACACCAGTTTGACCGCAAGTTGTTAGAACAATTCTTTGCGGACGGCTTGCGCTTAATCCACTATGACGTGATGGACCATTTTGTGGACAACACTGTCTTTCAAGGGGAACATTTAGATGAATTACAACAAATTGGTTTTCAGGTCAATGTACATTTGATGGTACAAGCATTAGAACAAATCTTGCCAGTATACCTCCACCATCAAGCCGTTAAGCGCATTTCCTTTCACGTTGAACCGTTTGATATCCCTACCATCAAACACTTTATTGCGCAAATTAAACAAGCGGGAAAACAAGTCGGTTTAGCGTTTAAATTCACCACTCCGCTTGTAAATTACGAGCGCTTGGTGCAACAACTGGACTTTGTTACCTTAATGAGTGTCCCCCCAGGAAAGGGCGGGCAAGCCTTTAACAGTGCTGTTTTTAATAACTTAAAACAAGCCCACAAATACCACTGCTCAATTGAAATCGATGGTGGGATCAAATTAGATAACATTCACCAAATCCAGGATGATGTCAATTTTATTGTCATGGGAAGTGGCTTTATAAAATTAGAACGATGGCAGCGCCAACAGCTGCTCAAAACCAATCAATAACTTTATATGTCTGCAACTGCTATTAGCGATCTCTTTGAAAAACCAGCCCAATTTAAAAACAAGAAAATTAAGCTAACGGGTTGATTGAAAAACAAACGTACTAGCGCCAACATTATCTTTTTGGAAGTTAATGATGGATCAACGCTGTTAAACCTACAAGCGGTGGTTAAACAAGACCAACCGGAGTTGTTTGCATTAGCTGAATCAATTAGTTTAGCGAGTGCTGTAAGTGTTTCTGGTACTGTAGCGTTAACACCCAAATCCAAACAACCCTTGGAATTAGTAGTCAAACAAATTAATGTATTGAGTACTGCGCGAGCAGACTATCCACTCCAAAAAAAGGAACACAGCTTGGAGTTTTTCCGCAACAACGCCTATTTGCGCGTACGAGCCCGTACCTACTTTGCCATCATGAAAGTGCGCAGTCTGTTGTCACAAGCCATTTTTGACTACTTCTTTAAGAATGACTTTGTTTTAGTGCACAGTCCAATTCTTACTAGCAACGACTGTGAGGGGGCAGGTGAGACCTTTGAATTAAAGCAAGGTAAGGAATTCTTTAATAAGACTACTTACCTTACTGTCAGTGGTCAGTTTGGTGCGGAGTGTTACGCCCAAGCCTTTAAAAAAGTGTTTACCTTCGGGCCGACTTTTCGCGCTGAAAAATCACACACTTCACGGCACCTTAGTGAGTTTTGGATGATTGAACCAGAAGTTGCTTTTGCCAACCTCAAGGACTTAATTAAGCTAATTGAAAGCACTGTCAAAACCGTGATTAAACAGGTTATGCAAAAAGCCAAGCAGGAACTCGACTTTTTAGAAAAGCAGTTTGATGTCAAATTAATGGAACGCTTAAAGCAAATTACAAGTACCAAAAACTTCCATGTTTTGGAATACACCAAGGCCTTAGAGATTTTAAAAACAGCCCAAGCTAGTGGTCAAGCTAACTTTGAGGTACAAGACTTTAACTTCGGTTTAGATCTCAAAACGGAACACGAACGCTTTTTGTGTGAACAGCATTTTCACAACCAACCGGTGTTTGTAATTAACTACCCCAAGGACTTTAAGGCCTTTTACATGAAGCAAAATGCTGATGGTAGAACTGTTGGTGCGGTTGACCTTTTATTTCCCCAAATTGGTGAGATCTGTGGTGGGAGTGAACGGGAAGGTAATCTAGAAAAACTAGTGGAACGTTGCCAAGCAATGCAAATTGATACCCAAACACTTAACTGGTATTTAGATATGCGTAAATGGGGTTACTTTGCCTCAGCTGGCTTTGGTTTAGGGTTTGATCGTTTACTAGCTTACATCTGTGGATTGGAAAACATCCGTGATGCGATCCCCTTTCCCCGTGCCCATGGTTCCATTAACTATTAAAACTTGACAGAAAAAGTTACCTAATTGGTTAACCATTTACCGCATTTTTATTGCTGTACCTACCATTATTTTTTTGGGATTAAATCACTTACTTGGTAGTGTTGCTAGTTTTACAGTGTTAGGCAATGTCACCATTCACCTACAGGTTAGCCTTTTTATTGGGGGAGTCTTGTTTATTACCGCAGTTATTTCTGATTATTTAGATGGGTATTGAGCGCGGAAGTGAAGGGTTGTGTCTAACTTCGGTAAGCTGTGAGATCCGTTAGCTGACAAGGTGATTATTAACGGTGTCTTAATTGCCCTAGTAGCATATGGCTACTTTCACTTTAGTTTTTTAATTGTGATTGTGCTGCGTGATTTAGTGCTAGACGGATTGCGCTTTTACGCTCAGGAAAAACAGCTAATTATTCCCGCCAACCAATGGGGTAAGTGAAAAACCACTTGACAAATGATAGCAATCTTAATGAGCTGTTTTGTGTTTAGTTTTTCTTTGAAGGAAACCAACAGTGCTAACACAAAAATCTTTTACTGAGCCATAGTGCATTTGCCCTATTATTTAGCTACAGCCTTCTCCTTGGTCTCGTTTGGTATTTACGCCCAACAAATATATAAAACCATTAAGGTTAAAGTAAAGTTATAATTTTGGAACTTTCTTATTGTTGTGTACGCGCGCTTAATTGCTGAAAAACTGCTAAATCATAAGCTCACCATTGCCACTGCAGAGTCAGTTACTGGGGGCCTTTTGAGTAGTAGCTTAACCGACATCGCTGGTGCTTCTCGTTTCTTTAAGGGTGCCATTGTGGCATACAGCAATGAATTGAAAAAATCACTTTTAAATGTCAAACAATCAACCTTAATTAACCACGGTGCTGTATCGCGTTATTGTGTTCGTGAAATGGCGTTAGGTTTAATGCAAAAACTCAATGTTGATATTGCCGTGGCCTGCAGTGGGGTAGCGGGTCCTGATGCATTGGAAAACCAAGCAGTTGGTAGCTTGTTTTTCTGTGTGATTGTTGCCAACAAGGCTTATGACTTTGAAACAAAACTACCAGCTGGCTCTAGAAACGAATTGCGCCAGCTTTTTGTGCAAAAAATCTTGCAAACAGTTGAACACATTTTAAGTGAAATTTCTTAGGTTAAATAAGGTTTAAGAGAACAAGATGCAGCGTACTTTAAATGTTGGGGTGATCCTGTGTGAAAGCTTTATTTCCAACGCCCAAAACCCAGTTAACTCCTACATTAAGATTTACGAAAACGTGAGGATGTTTGAGTTTGCGATTAAGCTACTTCAAGAATCGCGAATTAACTTCCAAAAGATCCTTTTATACGTTTTGGAAGAGCAAATTCCACTTGTGGAAAAAGTAGTTGCCAAGTACGAAAACTGCTGGGTCTTTCGTTCCAAGCACAACGAGGTTGAGGACATTTATGAAGCCAAGGGCTTTATTGAAGACAAGTACAAAATTGGTTTAAAAAGTTCTAAAAACCAAGCCTCTTCGTATTATGACAACTGTTGTTTTGTTGTCTTGGAAGCATGTCGACCTTTAACTTCTAAGAAGGTTGTTAAAAACGTGTATGAAAAGGCAATGATTGACGGTGCCGCTGTAGCTGTACTACCGTTTGAAAGGCAGCTTGTTTGTGGTGATAATACTAAGGCGGTGCGCCAGTTAAAGGACAAGGGCAACATGAACTATTGGCGTCAAAGTAGTACTTGGGAGTTGCAATTTCCCCAGGCTTATACGCTCAATAAGTTAAACCAGTATCATAAAAACCTCTTTATGAAGGCGCGGAACATGCTTGATTTAATGAACATTTCCGCTAAGAATCCCCTAAGCATTGTCGATGGTAGTGCTTACAGCTTTCGTGTAGTTACTAGCCTGGACTTTGAAATTTTGTTAGGTATTTTGCGCAATGGATAGAAAAATAGTTCAACTAATTCATAACTTTAGCGGTACAGAAAAACTAAAAGCAGCTGTTTTTTCACACGACGAACAGTGTTTCTTTGACTTTGTTAGCTTCGATGAATTAAACAGTAAGCTAACCGGGTTTCTGTTATTTGACAGCTTGGAGAAGCTGTTCAAACTAGTAGAAACAATTCAGCAAAAGCGCAGTTGGCTTTATGTTGATGAATTGTGACTGATCAATACTGCAACTGAAAACCAACAACTCAATGAAGTGAGCGTTTGGTTAGTGAAAAAGGAGCTTGCGCAAGTCGGTGTTTTAACCCAATTGGATACTAGTTTAGTCAAGCTTTTAATGGCTTCCAAAAATACTGACAGTGCCCTTTACAACACTTACATTAAACCAGTGGAGTTGCAACAGTTTACCCAAACACCAGCTCCTGATAACGTTAATGCAGAGCAATCGCATTTAACCTTGGAGTCTACCACCGACTTGAACAACAGTCAACTAGCCAATACTCCAGCGTTGTGGGAAGTGGAACAAACGACCCAGGAATTGTTACCAACCATGGACTTTTCTAAGTTTATTGATGAATTAGATCAAATTACCAAAAACTTTTCTGATTTAGAGTTAGAACCACTTAGTTTTAACGAAGGTTTTGATGAGTGAAACCAAGAGTAAAGTACTCGTTTTAGGCGGTTTAGGTTACATTGGGAGCTGTTTTATTGACCAGCTTCTAAAACAGTACCCTGATGTAACTGTTAGTGTAATTGACATTAACCACACTAGTCTGGCGCTACAACTGTTACCAAGACAGGTCAACGTTCACTTTGTTAACTTACTTGACCGTGCACAGTTAACAGATACGATTGCTCAAATTAACCCTGATGTCGTGTTTCACTTTGCGGCAAAAACAAGCGTAAAGGAATCGACCGAACAACCACTTACATATTTTGACCATAACCTAGTCGGTACGCTCAACTTACTCCACGCTTTAAAAGAACTACAAAAACCGATCCAGCTGTTCTTCTCCTCCACTGCTGCAGTCTTTGGTAGTGCTAGTACACTTCCAATTCCAGAAAACTTAGTTTTAGAGGAAACACTCGCTTCCAATCCGTATGGCATTAGTAAGTTTTTAAGTGAAATAGTACTGCAAACACTTACCCGTAGCCCCCATTTTCAAGTAATAGCGTTGCGCTACTTTAACGTAGCGGGTGCTAGTAATCCCTTTGGTAACTTCAATAAAAACACGACCTTACTGATTCCTAACCTGATTAAAGCCTTTATGGAAAAGCGCACCTTCTTTCTGTATGGTGATGACTATGACACTAAAGATGGGAGCTGTATCCGTGATTACATCCATGTAGTAGACTTGTGTGATGCCCATTTGTTGGCGTGAAAGTGGTTGCAAGCCAATCCTAAAGTGCGCTTTGAAAGCTTTAACTTAGGTAGTGGTCAGGGCTTTTCCAATTGGGAGGTCATTAATACTGCTCAAGCAATCTTTGCGCCTGAACAATTACAGCTCAAAATTGAATCACGAAGGGCTGGTGATCCACCTGTTTTAGTGGTTGATTGCACGAAGGCAAAACGCTTACTCAACTTCCAACCAACCCGCTCATTACACAAAATGCTGAGCGATGAAACAATTTTTTATCGGGATTTTTATAATAGGCTTTAAATTCAATTTTGGCAATGTCAAAAATAGCCTTCAGGATGGAAAATATCTGCAAGAGTTTTGACAATGGTAGGGTCAAAGCTAATGTAGATGTTAACCTCACGGTTTACGAAAATACTGTCCACACATTGCTGGGTGAAAATGGTGCGGGCAAATCTACTTTAACTTCCATTTTGTTCGGTTTATACCAACCCGACAGCGGCAAAATTTTTATTGGTGAAGAGGAAGTACACTTCAAGTCCTCCAAGGATGCCGTGCAGCACAAAATTGGGATGGTGCACCAGCACTTTAAGCTGGTTGATAACTACACCGTCTTAGACAACATTATTTTGGGCAATGAAAGTAGTTTTTCCATTCCTTTTACCAACGGTAAGCTTAAACTACCTTTATTGCACCGTAAAGCTAGCGAAGCCAAAATTCAAGCAATGATGGAACGGTATGACCTCCATGTGAACTTGCACCAAAAGGTAAGCCGCTTAACGGTGGGTCAGCAACAACGGGTCGAGATCTTGAAGGTGTTATTCCGTGACAGTGACATTCTCATCTTTGATGAACCAACCGCTGTTTTGAGCGACCAGGAGATCAAGAGCTTTTTAAACATCATTAAAAACTTCAAGAAGATGGGTAAAACGATTGTGCTCATCTCCCACAAACTCAACGAAATTAAGGAAGTAGCGGAGACTGCTACCATTTTGCGTCAAGGTCACTCCGTTGGCACCTTCCAAATTAAGGACACTTCAATTGATGAAATGGCACGTTTAATGATGGGAAAGGAGTTGAAGGAAACCAAAAACAATACTCAGTTTACCGCCAAAGGTGAGCCCGTTTTAAAGGTCGAAAACCTCCATCTTTATTTAAACCAAAACTGGTTTTACAAGTTAATTGCGCGCTGGAACCAAAAGCGAATTAATCAACTCCAAAAACAGGGTAAGCCAGCGAAAACACTGTGGTTAAAGAGTTGACTAGAAGGGTTGGCAGCTATTGAAAAAACACCCCGTTTTATTCGTGGAATCGTTAATAATTTAGGTTTTGGTAGCCAACAGGTGTTTGATAAAGGGATTAGTTTTGAGATCCATAAGGGTGAAATTTTTGCCATTGCCGGGGTGGAAGGTAACGGTCAAAACCAATTAATAGATCTTATTTGTGGCTTGGAAAAAGCAGCACCAAAAAAGGTCTTCTTCAATGGTTTTGACATTTCACGTTATTCCATTCGCAAGCGGATTAACGCTGGTATTGGTTTTGTGTTAGAAGACCGCCATAAATACGGATTAATCTTAGACCAAACGGTGCGTTTTAACGCGGTGAATAACCAAATTGATCGTAAGCAATTTAGCAGTTGAAATTTTTTAAATCAAATGCAAATAGCTGTTTACACTAACCAAATCGTCGATAAATTTGATGTTCGTGGAGCGGTACAAGGTACAGCAATAGTGCGTTTACTTTCCGGGGGTAATCAGCAAAAGCTGATTATTGGTCGGGAACTGACAAAGCAGAACGAGCTGTTAGTGTTTGCTCAAGTAACCCGGGGTTTGGATGTTGGTGCCATTTCGTTTATCCACCAAAAGATTTTGGATGCAAAGAAACAAAATAATGCAATCTTGTTAGTGTCATATGAGTTAGATGAAATTTTAGCTATTGCCGATACCATTGGCGTAATTAACAAAGGCAACCTGTTGGAAGTCAATAAACGTGATGTAATGACGCGTGAACGCATAGGCAAACTAATCATGCAATAAGAAAAAACTGGGAATTATGAATGCTTTAAATTACCTCAAACACCGTTTTCTGTTTAGTAAAGACAAGTTTTGGTACGCCCCTTTTAAACAAAAGCAACGTCGGAGCATCTACTCCACCTTTTCGTTGATTGTTCTCTCCTTTATTGTTTCGTTCTTTTTAATAGTCGCCATTCCCGGCATTAAAGGCGGTACCTTCTTAGAAATCTTTACCCGTTTATTTAAAGATAGGATTAACATCGAAAACTTTGCACGACAAATTGCGATTTACACCCTAGCTGCTTTGGCGTTTAGCTTTTGTATGAGTGTTGGGGTGTTTAACATTGGGATTTCGGGACAAATGATGGCTGGCGCTAATTTTGGTTTCATGATGATCCTCAAGGTTTTCCCCGAGTCATTCCGTCCTGCCTTTGGTGGTCAGATCATTACCATCCTCTTAATGATTCTAGGTAGTGTGACCGTTGCTATGGTGGTAGCTGCTTTAAAGGTCTTCTTTAAGGTTAATGAAGTGGTTAGTGCCATTATGCTCAACTGGGTAATTGTGCTGGTTAGCGCTTATCTAGTGGGTACTTACATTAAACCGGAAAAAACTGATACTTCCCAGTTTTACTCGATTGAACTACCGGATGCCTTTGCGCTGTATAACTTCTCTGATGTTCAACAAAAGTATGGTTGATTAACCTCGTTGGTTATTGCCATAGCAGCTGCCATATTCATTGCAGTTTTAATGAAGTTCACCGTTTTTGGACACAAACTCAAGTCCACGGGTTTAAGTGTGACGGGTTCGCAAGCCGCTGGTTACAGTGTTAAGAAGTATCAGTTCCTCTCGTTTGTGATTTCAGGAATCCTTTCTGGTTTACTAGCAGCTGTAGTTTATACCGCATCCTTTGAAAAGCAGTTAACGTTCTCAGATGTGGGTGACTTTGGGATTACATCGGTACCGATTACTGGGTTTGATGGAATTGCAATTGGTTTAATTGCGCTCAACAGTCCAGCGCGAATTGTAATAGTTTCCACCATTATTTCATTTGTAACCATTGGTGCTAAACCAGCGGGGTTAAACGCTGCTACTGCGAGCTTGGTGTTGGGTATCATGATGTATTTCGCTGCCATTTATAACCTGATGATTTACATCAAGCCATGGCGCATGATTGTCAAGCTCAATATTAGTAAGATGAACGAAGCGGCATATGATGAATTCCAAAACGAGATGGCTGCTAATTTAGAAACGCTGTCGTTCCAGCGCTTTTTAGATAAACAAAAACGGAAGCATGATAAAGAGCGAATGGTTTGGTTTGACACCAAGCGCTTTGAGGAGTACCAGAAAAAGAAGCAAGCAACTCTACAAACCTTCCATGAAAATTCCTCGCAAAACTTACTGCAGTACTGAAAGCAACAGTTACTAGTAGCCGATGTAAAGCGATTAACTTTCAAATGAGACTTTCTTACCTTTAAACACCAGCAAAAGTACATCTTACGCTGGTACAAGGGCAAGAACAAAAAGCAAACAGCACTAGAAAATGAGTTTGCTAGTTTAAACGAAGCTATTAGTCAAAAATTGGAGGAGAAATAATGTCACAGTCTTTAATTTCTTTTAGTAACTTAGATAACTGGTTGTTTGTCGCTCCTGCACTGCTACTCGCTGTATTGAGTGGTTACTTATCTGAGCGGGTTGGTATTGTCAATATTGCCATTAACGGGGGCATGGTGTTTGGCGGGATGTTCTTATCCTTAATGTCTTATGCCTTTGTGCCCAATGCAAATGATTCTGCACCATCTTGATCACTAGCAATAAGCATCCCTTTAAGTGTTATCTTTGCCTCAGCTGTTGGTTTTCTGTTTGGCATTGCCGCCATTAAACTCAAAGCCGATCACGTGATTGTTGGCACAGGGGTTAACTTACTGGGCACGGGAATTAATTTCTTTGTGGCACAAAATGCCCGCTCATTGCTTAACGATACTGATTTAAGGGTACGATACAGCTTTGTAAGAACAGGAAACTCTGTAAGTATTGAGGGTATAGCAATCTTTGCCTTTGCAATTATCTTTGTTTTATTAGTGTGGTATTTGATGAACTTTACTAAAACGGGCTTGCGTTACCGTGCTGTTGGGGAAAATCCTAATGTGATTGATACCCAAGGTATTAGTGTGTATAAATACCAGTGGTTTGGGGTAATGGCTTCGACGATGGTAGCAGCTTTAGCGGGTTGTTGTTTTGCTTTAAGTCCACAGGTACCTAGCTTTTCTAGCGGGGATGTGAGCGGGTTTGGTTTTATTGCCATTGCCATTATGATCATTTCGATGTGACGAATTATCCCAAGTATTGTGATCTCGCCCTTGTTTGCACTAGCTTATGTGCTTACTACCGGTGTGGTAGGTAATGCTAATAACACTTACTTATTGAGAACTATTCCGTTTATTATTTCGCTGTTGGTAATGATGGTATTTGGATATCTCAATGTAGGGCCGAAAAACGTTGGTAAGCATTTCGATAAGGGTTTGCGATAGGTTAACCTTTATTTATAGTTATAGTCAAGCCAACAAATGAGTAAAAATCTGGTGGTGATTGAATCACCCAATAAAGTTAAAACGTTGCAAAAATATTTACCGAATGACTTTGAAATAGTCTCTACCATTGGTCATATTCGGGAAATGGTGCACAAAAACTTTGGTTTTAACGAAGCCGATTACTCACCAGTTTGGGAAGACTGAACTAAATCAAAAAAGAAGTTTTCTAGCCTTAGCTTTAAAGGTAATCTTAAGGGCAAAAAACTTTTAAGTAAGTACGACATCATCAAAAGCATTAAAGAGAAGGCTAGTAAAGCCACTAACATTTACCTAGCAACCGACCCAGACCGTGAGGGAGAGGCAATTTCCTGACATGTTTACGATGTTTTGGATGAAAAGGACAAGTCGAAGTGCCAACGCATTACCTTTAACGAAATTACCAAGAACGCCGTTTTAGATGCACTGAAAAACCCACGGGAAATTGATCAGAGTTGGGTGCAGAGTCAGTTTGCGCGCCAAATTCTTGACCGGATGATTGGTTTTCGCCTTTCAAGGCTGTTAAACAATTACCTCTCAGCTAAGTCAGCGGGACGGGTACAATCAGTAGCACTGCGTTTTTTAGAAGAACGTGAACAGGAAATTCGTAGCTTTGTACCACGGTTTTGGTGAACGCTCGATGTGTTGTTAAATCCCAAGGCTGAAGGTGTTAGGGAGGCGTGTGCTAATCGTTCAATTCCAATTGTCTTGCGTGAAATTAATCCTGCTTTACGTGCTGGCTTGAAATTTGAAGAGGAGAAGAGCGTATCGGGAATTGACTTTTTAGATGAAGCATCAGCTAAAAAGTTTGGTGAACAGCTCAAGGGTGTCTTTGAGGTGTACAACATTGATGAAACCAAACACTACAGCAGTTCGCCCAATTCCGCTTACACCACCGCTTCATTGCAAAAGGATGCCATCAACAAACTAGGTTGGTCTTCCAAAAAGGTAACCTTAATTGCCCAACACCTGTATGAAGGGGTGAGTATTAATGGAGAACAAACCGCTTTAATTAGCTATCCCCGTACCGACTCAACTCGTTTATCAGCGCAATTTCAACAAAGTTGTAAAGAATACATCTTAAACCATTACGGAGAAAAGTACCTCTCTAATCGGATTGTAAGCGCTAAGGGAAAAAAAGGTGAAAAAATTATCCAAGACGCCCATGAAGCAATTCACCCAACTGACATTAACATTACCCCGGAAATGGTCAAGAATGCGATTAAGAAGGATGAGTTCTTACTGTACCGCTTAATCTGGATCCGTACGGTCGCTAGTTTAATGGCGGATTGTAAAAAATCACATACCCACATCCGGTTTATTAACGATGGTAATAAGTTTTACGCTTCTTCCAAGTCATTAGTATTTGATGGTTATCGCAAAATTTACGAACACTTTGAAAATAAGGAAAGTAATGATCTCTACATTGATTTGGATAAGATTAGGGTTGGTGATCGTTTCATGGCTAAAGACATTAAGATCACAGCCCGCCAAACCCATCCAGCAGCCCGTTATACCCAAGCTAGTTTAATTGAAGCGTTAGAAAAGTCCAATATTGGTCGACCTTCCACCTATAACACGATGGCTAGTGTTAATTTAGACCGGGGTTATGCTAGTTTAAATAAACATGCTTTTCACGTAACACAACTAGGGGAGCAGGTTAATGAAGAATTGTCCAAACACTTTGGTAAGATCATTAACAAAGAGTTCACTAAAAACATGGAGAAGTCTTTGGATGAAATTGCTGAAAATAAAAAGAACTACCAGGAGTTTCTCCGTGATTTTTGGTCAAACTTTAAGGAAGAGGTAAAGCTAGCGGAGGGTTCGATTCAAAGGGTGAAAAAGGAAAAGGAATTTGTTGGTCGTGATTGTCCAAGCTGTGCTAGTCCCTTGTTGTACCGCTATACTAAACGGGGTAATGAAAAGTTTGTTGGGTGCAGTAACTTTCCCAACTGTAAATACAACGAGTTTAGCCAAAATAAACCGAATTTAACTTTGGAAAAGCTCGAAGAACTGTGTCCGGAGTGTAACAGCCAACTAGTGAAAAGACGCACTAAGTTTAATCCCAATAAAACCTTTGTTGGTTGTAGTAATTTCCCTAGGTGCCGCTACATAAAGAAGGATAATGCTAGCTAGTTTAACGAGCAGAAGTGCCACTTCCTTCTCAATAATTTGAGCGTTGGTAAGTGCCATTTTGATCTTAAGTATCCTCATTTGGCTTATTATCACCATTTTCTTTGCTTGAAATTTACACCTCAAAAACAACAAGAAACGAACAAAGTACCACTTAGAGCCGGAACAAATTAAGCACAAGATCATCCAAAATAAGACTAAATTGGGCAAAATGTTGGACTTTTACCAACAGCAGATTAACACTACTGCAACTGAGCTGAAGTGGTTGGATGGTCAGTTTCAGCAAATTGATGAAACCGATAAAAAGAAAGCGCATCAAATTGCAATTAGACTAGCGCGGAACCAACTGTTACAACAGCTTAGTGTAAAGTTAGACCAAAAGCAGTTTAGTCAACGCGCTAATAATGAACTGCAAAAGTTAAAGTTGTCTAACTTAGAGAGCTTTACTAACCAAAAGATTAAATGAGACCAGGAGGGGATGAAATCCGCTGTATCCCGCGTTACCATTAACGAGTGAACCTTTAACCATTTTGCTGGTAAAAACCGTGTTTACTGGGATTACTTTAAACAAGTTTGTGATGTGGATTGCTCGATTAAACCACTTAAAGATCAGTTAGAGATTACTTTTTCGAGCTGAAGCTTATTGAAAAGACTGCAAGCAAAAAACCTGTTTAATAAGTTAATTGCTCAAAGTAGCAGTGTCAAGATGAGCGAAAAACTGATTAACAATGCTTTGCAATTAGTTCAGGACAATTTGGCATTACAAGCGAGTGAAAGTGGCAATAAGCTGCTCAAGGAGTTTGAATTATCGTGCACTAACACACAGCTAGTCCAACTGTTGGGCTTTCAGCAGTTTTACTTCGGTACAAACCTGCTTAGCTTACTTGATTTGAGCCGTTCAATCGCTGTTTTAGTGCGCTTTTTAAATGAGCACTGTAAGTGAGAATTAAACGAACGCTTACTAGTGGAAACTGCTTTGTTTAACAACTTGCAATGGGTTAATAACAACGACTTCTTTTTAAAGTCACACAATGATTTAAAACAGCTGCACCTCTCCGCTGAACAGTTGGCGATTATTGAACAGCAAAACCGTCCCTTTTACATTGATGCTTATGCTTTATTGATTGCTGGCGTAAAGCAAATGCTGATGGAGCACGATGCTGTGGAACCAAAACAAATTCACTTTCATAATGCCAAAAAGGTAATGGAAAGCTTCCAGCTGTTTGGCATTGACCAGTTAGCGTTAATAGAGTACAACAACTGTCTCTATGGCTTTGTCACCACTAAACTCTATGAGATTAAACAATTAGATGATTTAGCTTTATTTAAAGTTTTGTTTAAAAGCTTTTTGAACAAGCATTTGAAACAAAAATTTGCAACAATCAGTTTATTTGTAAATACCCAAACACTCATGATCTAACACTTTAGATCATGGAAACAATTAGCGAACTTTTAGAATATAAAACAATTGAAAACAAATAGTTTATGGTAACTGAAATTAAAAGTCTCAAACAACTCGGAGAGTTATTTGCTAGCAACAATAAGGTAATTATTGATTTTTGAGCAGAATGGTGTGGGCCGTGCAAAATTACTGGACCAGAATTTGCTAAAGCTGCTAGTGAAGTGAGCACTGTAGCGTTTGCCAAGGTAAATGTCGATGAACAAACTGACATTGCCGCTGCTTACAAAATTACCTCACTACCTACAATTGTTTTATTTGAAAAAGGCCAGGAAAAGCACCGTGCTATTGGTTTTATGCCTAAAGCCAAGATCGTGCAACTTGTCAGCCAATAAAACTTCGTTTCATGATTGACTTGCTCGGGTTAGATCTTGATGGCACTTTGTTGTCTAGAACAAGGCAAATTAACGATCCTACTAAACAAGCTTTGGCTAATTTAATCCAAAAGAAGCCTAGCTTAAAGGTAATGATTCTAACCGGTAGATCATTATTCTCCACCTTGAAGTACGTTCAAGAGCTCAACGAGCTGTGCAAAAAACCGCTAGTGGAGTACTTTTGCTGTTATGGTGGGGCTAAGTTGTACCAACTCAATAACAACCAACCCCAAGAACAGTACAAGTTTTTAATTGACAGCCGGCAAGTTAAAACAGTGTTTGAAATTGTGGAACAACACAAGGGTTTGTTTTTGGCATACCTCGATAAACCAAAGGCACCCTACATTATTTTGGGGGCAAACCAGTTTTATGCTTGACTGATTAAACAGTTTTGGTACAAACAGCGTTGTGAGTACTTTAAAAATGATCATTTAACCGATGGCATCTTAAAGATTAATGTGTACTTCGCGTGTCCTTTAAGGTTGAAGAAGGTGTACCAGATCATTAAGCGTCAGTTTCAAGACACGCTTAACGTTGTGAATTTTTCCAAGCACTTAATTGAAATTACCCACAAGGATGGAAATAAGGGCTATGCAATTGAAGCAATTGCCAAAAAACAAGGACTTTCGTTAAAACGGATGGCGGTGATTGGTGATTCCTTAAATGACCGTTCGATGTTTGAAAAGGTACAGTATTCGTTTGCAATGTCGAAATCACCCGATGAACTCAAACTGTTAGCCACTGAAATTGGCACTAAAACAAACCGCTTTCGTTTCAGTAGTTTAGTTGATTTAATTACCGAAAAAATTATTAACTAAAACTTTTTTACTTGATGTTTTGGCTTCCTAGTCCAAAGCCATCATAGAAAAGTGCTAGTGTTGTTCTGGCCGTTGCTTGGGCTTGATTTTTACCCGCCTGGAGGCAGTTAAAGACCTGTTCACGACTAATCTGTTCCCGTTTTCTCTGAATGTTAACTAGCAGATTAACGGTTGCTTCTGTAAGTGCGTTTTTGAGATCTAAATAAGATTTAGTACTAAAGTAAGCTTCTAGATCATTCCCTAACTGATTGGTTAAGGATTGATTGACAGGCTCTTTTAAAAGTGCCTTGAGAATTGTGAGCATATTGGTCACCCCTGGCTGCGTTTTGGGAGCAAAACGGATTTTGTTAAAGCTGTCAGTGGTAGCTTGACGAATTTTTTTAACCACTACTTCTTTTGGGTCATCCAAGTAAATTACTCCGTTTTGGTTCTTTGAGGACTTACTCATCTTTTTGGTTGGGTCAAATAAGTCCATAATACGGTTGGTATCTTTGTTTTGGACAAACTGGGGTAGACGCAGCTTGAGCTTAAACTTCTTTTGAATTCGCTGCGCTAAATCACGAGTAAGCTCTAGGTGTTGTTTTTGGTCATTACCAACGGGCACAATGTCAGGTTGGTACAGCAAGATATCTCCCGCCATTAGGGCTGGATAAGTTAACAGTCCGGTGGGAATGTTTAAGGTGCCGTTTGGGTTACGGGTTTGTTCCGCTTTTTTGGCCTTGAACTGCGTCATCCGTTGCAGTTCACCGAGATTGCTTTGCACCATCATTAAATAACCCATCATACTGTGCTCCAAAAGATCGCTTTGTAAAAAGAGACAAGCTTTTTGTGGATCTAGACCAACCGCTAATAAAGTACGTACCAAATCAAAGTTATTTTGTTTCAGTGCTTGGGGTTGAAAATCAACGGTAATTGAGTGTAAATCAGCGACAAATACAAACAGTTGGCATTGCTCTTGGAGTTCAATTAACGACTGCATTACCCCCAAATAGTTGCCTAAATGCTGTTTGCCCGATGCTTGAATCCCGGTAAGAGCGCGTTTCATCATATGATTGCAATTTGTAGAATCTATTACAGATAGACAAACGTTATTTTAAAAGAAAAATGCTTAAGAAAAAAGTTAATAATGATGCTGGTAAGACGTTTATCTTAATTTCATCATCTTGCTCGTCTTGCCAAAAGGCAATCGAATACTTTAAGGAAAATAATTTGAGTTATACGATCGAAAACTTTTACAAAAAACCGATAAGTGACAAGCGCTTTCGTGACATTTTAAGTTTGAGTGAAGATGGCACCGAAAGTCTGTTTTCCAAGCGTGCTGACCAAATTCGTAGTAACACTAATCAATCAGTGGAAGATTTGACCATTCCTGAATTGATTAAGTTAATTCGCGAACGTCCTTCAATTTTGCGTCGACCAATTATTATCCAGTACAACTCTTCGGGGATTCCCAAGCGGATGCGCATTGGTTATAACGCCGCTGAAATTAAGGTATTTGAACGCAGCTTAATGGAACCTAAAGTGCGTACAGTAAAGAAGTAATGAAGTACAAGATTTTTGCATCAACAACACCACAAACCGAGCCAGTTTTGCAAAAGTTAAAGCAGGTGTTGAAAGGTTGTGAGGCAGTTGAAAAGGGCTTTGATTATCTCTTTGTATTAGGTGGTGATGGTTTCTTTGTTTCTACTGTGGCCAACTATAACTGCCACAATTGTAGGGTAGTAGGGATTAACACTGGTCATCTAGGTTTTTACACATCGTTTAATGAAAAGGATTTGGATGATAATTTCCTCCAAAAGCTCCAGCAATGTCACTTTCAACGGATTAGTTTGTTGGAAGTTAGTGTCAATGGCCAGCAGCACTTGGTTTTGAATGAACTGGCAGTTTACACCAACACAGCTTACCCAATTAACATCTTTATTGATGGTGAAGCGTGGGAGTTTTACCGCGGTTCAGGTTTATTAATCGGTCCGCGTACTGGTTCGACTGCACTGGCTAAATCTGCTAAAGGGGCGGTGATTTTCCCTGGCATTGATGTGCTGCAAATTATTGAAATGAATCCACTTTTGCACCCCAATCAAGTTACTATCCAATCACCAATTATTTTGCCCAAAGAAACGCAAGTGGAATTTGTGGTCAAAAAAGCCTTTAACCCACAACAGTTTCCCACCTTTTACTGTGACGGGAGAAAGTTGGAATTGCCAAACGCTGACACAACTTTAGCGCTTAAACTAGTGCAATCCACACCGATGTTTAACATTTCACTCAAAACACAGGACTTTATCAATAAACTTAAGTCCACTTTTATTAAACAAAGCTAATGAAAGTATTGTTGTGAATTGGTTATGTGTTGAGCTTTGGCTTACTTTACCTTTATTTGGTAAAACGTGCTAAGAGGGCCGCGCTACAATTAAACAACAAACTAGTCGAATCACACACAATTCCTTTTGCTGTACGTGACTTTATTGCCGCTTGCGGGGGTAGAACTAACTTTGTTAGTTTACGCACCTCACCCACCCAATTAATTGTTAGTTTTGCCAAGCCAGAGTTAGTTCAAATTGCCGCTTTGCAAAAGCTTGGAATTAAAGGGATTAATAAGAGTCAGAACCAGTACCGCTTTGTATTGGGCAATTTTGTCAATCAATTAAAACAGCAAATTGAAAATGAGCGCTAAGTTAACGTTAGAAAGCATTGCAAAAACATTTGCCGAAACATCGATCTTTGCGATCTTGTTTTTAATTATTGTCATCCTCAATTTAGGTTTACTTGTTTTTTTAGCGTATCAGTACCGTGTTTACAAAAAGAAGCAAAGAGCAAACCTCACCAAGCAGACTTATAACAATGACTATGGCCAAATTGTTAATTTAAAACAGCAAAACGGTGCCAAGATAAAGGAGCTTGCTAACTTAAAAGATCAGTTAAGTGAATTAGGCCAAAAATTTAACACCACACTGAGTGAAATCATTAACAAACCACTTGTCAATGTGATTGATGAATACTTGGATGAACAGTTTAAACAAGCTGCTAATTTTCGTGAAGCAGAACTTAATGCCGTTTTAGATAGTAATGACCAGAAAACAGTGTTCCATAAGCGTTTGTTCAATAAATTTCACTTTGGTGTCGACAAGCTAGCTAATATTAATGTCAAAAATCCGCTCAATTTATGCTGGGTTGACAGCGCTAGTTTTACCGTGATTGAAAGTGATTTCCGCAAACTTAATGGTGTTGGCGGGATTAATAAAAAGTTGTTAATTGAAAAGTTAAGGATTGAAGATATTATCTTTACCAATATCGATAAGAAGTACTATGAGGTACAGATACTATCTGACTCGCCAGTAAAGGTACAAAAAACAGTGTTAACTATTCGCAACATCTTGATTAATGATTACGTGGACAATGAAAAAATTGAGTCATATGCTAGGGAAGCTAACGGTTACTTCAATGACCACTGTAAACTGATTGGTAAGCAGGTGTTAGAACGACTCAATATCTTTGAAATTAGCCCTAAACTACACAAGTTTTTTGGTTTATTAGCGTTTCGTTATTCTTTCGGTCAAAACGTTTTATCACACTGTCTGGAAACTGGTTTTCTCACCGCTTACCTAGCGCTTTTAGTTAACTTTAAACCTGATGTTGCATTAAAGTGCGGTTTGTATCATGATATTGGTAAAGCGGATGATGAAAACGGGAAGAAGAACCACACGGTTACAGGTGCCAAGATCGGTGATGAATTTTACTTTGAAAACGATGTCAAATACACAATTGCCAACCACCATAATAAAAATGTCGATAACGTATACTGTCGTTTAACTCAAATTGGCGATAAGCTTTCAGCAGGGCGTTTGGGTGCGCGCTCTGACAGTTCGGTTTTATTTAGCCAACTCAAACAAGAATTAAAGCAAATTGTGGAAGAAACACTAGCACAATTTAAAACAACCATCCTTTTAGGACAAAGTGGACGCCGATTAGTAATTTGATTGGAAACCAACCAGCACAATAACATTATTGACAACCAACAGTTAACTGATTTGGCTACTACCATTAAAAGCAAAATCGTGCAAAACAACATTACTAATCGCTTTCCAATTAAGGTAGTATTACGCTATAACTTCGAACACAGCTTTGATACAAAGGACAAGAACTAGTTATGCAATACACCGTTTTAATTCCACTGTTTATTTTTATAGGAGCCATGGTGTTGTTTGGTTTTAGCTTTCAGAAAAAGCAACCACAACGCCGCATCGTTCAAATTCTGTTCTTAGCTTACTGTGTGGATTTTTTAGCACTAATTATTGCAGTGATGATGCTCACATTCTTAAGTTATGATGACTTAATGCTGGGCGTTTTAATTCCAGTATTAATCCTTTCAATCATTATGTTCTTTGTGATGATTATTGCCCACTATCCACTAATGAAACGCTTGTTTGGACATTAGACAAAACGTGTCTCATTCAAGTTGTGCACAAACTTAGGTGTTTTAAGTAGATAGTCGAAGCGTTAAAAAAGTATTTTGGGTCTTCTACTTTTTCCCTAAGTAGATGTTTTTTATTGATGTAAATAAAAGGCCAAATTTAACTCTTATGAGTTAAAAAGTTCATTTTTTAAATAACATTAAACCGGAAATTTCAATTAGTTTCCTCTTCTTCAAAAGGATTTTCCAAAAAAAGCAAAATAATGCAATTATTTTTTACTGTTAATGCGTAAAAAGAAGTTTTTGTCAAGGTTTTCTTTTAGTTCTCTATTTTTATTATGTGGAACATTGTTAAGTGCTTGCACTGGTATCCAAGCTGATCTTAGAAATCTTATTAAAGAAACCACTGGTAAAGACATTGATTTGTCTAAAGCAATTAAAACTAAAGAAGGCAAAAAGAACATAATAGCTTCTTTAAAAAAATCTTACGAAGTTAATCCGAAAGATACAACAAAATTGTTGTTAGACGCCTGAAAACAGAGTTTTGAAGAGGGTAAATTAGGTATTGCGGATTTTGATTTTGACCATGTAGCTTATCCTCAAACAAATGATCCTTTCACGATGGAGCGAAAGGTTGATCATTTTCAAATGACATACCAAAGTTTTAAGGATTTGTTAGTTGAGGCTAGGCTGAGCTATACCTTTAATTGATTTGGGGATTATTCTTCAGGTGATTTCACTGCTAAGAGAGGTGACAAGCATTATTTTTATCTTTTCCTTAAAATTAAATCTGATCCAAAAAAACAATTTTCAGCAAAAAAATTTCTTACTGAAGGAGAAGCGTTTACTGATCAAGAAGGAAAACAAACTACAAGAAATTTAGAGTGAATTGAGTTTAGCGCTTCGATTAGCTGATGAACAAAAGGCAAAGATGATGTTAGTCAAAAAAGTTTGAAGAAATTTTTAGAAAGCTTTGCTACTAACACAGGCTACTCATCAGATATTAATCTCTTTAGTTATTTGGAATATTTAATTAAGTAATTTGTTGTGATTTTTTAGTTTTACTTGGAAAAAATTTAGCCACCTGCTTTTCAACTTGAACAAATGGTTTTTTATTTGTACCGCATGAATAAAAAACCAGCAACTAACTTTTACAGATTAATTGCTGGTATTCTTTATTTTGAATAATATCTACTTAGAATCTAGTTTATCTAGGCGAACATCCATTGAATCCAAGCGATTGCTCATCTTTTGCAACATTTGCAAGATTAACTGTAGAGTTTCTCCTTGAACTTTTTGCTCCGCTTGAAGTTCTTTAATTTGTTCCCCTTGAGTTTGAACAGTTTCGGTTAATTGTTTAATTTGCTCACCTTGAACCTTGATCTGTTCACCCTACGTTTTTTGCAGTTCAAACACAACATCAACTTTACCTTCAACGCGCTTCAATCGGCTTTAGTGGCGAAGTCTTTGAGCTTTTCGTCAAGCTATTGGTGAATTACAAATTCATATGTTTTTGACACCTTTTTACTTCCTGAATTAGGATCATTAGGACCTTTTGGAAATCCTGGTTATTTAGGGTTCTCAGGTTATTCTGGTTTTTCTAGAAATTTTGGCAACTTCGGAGACTTTGGAATATCCGGTGTATTCGGATTCTCAGGTTTATCCAGTTGATCTTCTATTATCGTAAAGTCTTTATCCTTAAAATAGTACTCTTAGGTAGCTTGTGCAAACCATTTCTTTGCTTGGTATAGACAATTTGAAAATTAACTCTTTCCTTTGTCCGGGAAAGGATATTAAATTCCTTTTAGGAAATAACTTTAGGCATTAATAAATTCCTCCTTTCTGTTCAAGGTTTAGAAAACTAAAACGTGCAACTTTGTTGCCAGCAAGGATCAACATCATCGTTGATCTAATAAATATATTGAGTAAAGGCATAATTTCCCTTCACTAAATAAATTGAACGCCTTATTTTGAAATGACAACAAATTGACAAATTTCTTTTTAATTAAAGAAAATTTTGGAAATTAAGAGCTAATGCTTAATTTAAAGTCGTTGAATTTAAGCCTTCTTTTTGTTTTTGAACTCAATCGAATAAATAGTTTTGGTAATAATCCAATTGCTTTTTTACGCATTTCAATTTCTGTGGGAATACTTTCGGTAAGATCGTTACATTTTTTTCAAAAGCAAAGAGGATATCGGCAATCTTTTCTTGAATTTCTAGAGGTGGGAAAGTAAGTTCAATTTCTGCTAATTTACTTATTCCTAATTTAGGAATAGGGCAAGCATAATTAACAAACTTAGATGTTTGCAATCTGAGTGCATAAGCTAAAAATTTAGGATTAATTTCTTCGTTTTTTACTTTTAAAACGCCACAGTCTTGTCTTGCATTAAACTGTCCTTTTCTATAGAATACAGCACCAGCATAAGAACCTCCTGTAGTTCAAGTCACGTACTCGCCATCTCGGTCACAATCTTTAATTTTGCCAACTTCACCATCATTTAAGGTTGAAGCTGAATAAACCGGGTATTAACCGAGATTATCCTTAATATATTTTTTAGTAATTGCACGACCCCTCGAGATCTCGCAAATATCTTTGATTTTGTAAGTTTTGATTTGCACAACAGTAACAGTTTAATTTCTTTTAAAAGTCATTTTCAGCGACTGTTTTAGATTTTTCTTTGTCATCATTATTGCGCTCTTTCTGCATATTAAGTGCTTGTTGGCAAATTAGAATAATACCCAGTTATGAACCGTCCTACTCCCAATTTTGAAGCAATCGATAAGAAGATTAGTGCCTTTGTCACTAACCACGATAACTTATTGGATAAGTTACTCAAACAACAAACCGAATTGTTAACTAGTGAAATTACGACTAATTTTGAGGTAACCCAACAAATCCAGGAAGAAGTAGCCAAAAAGACGAAGCAACACTCTAAAAACTACAAGTGACTAGTCACTGTTGTTTTAGCGAACGGTGTTGTAAGTCTCTTTCTTTTAGGTGGGTTAATCTACCTCTTTAGTAAGTAGTTCCCAATTAGCTTCAATTGAGCGGTTGTTCGTCTTTCTAACGTTGTAACAAAAGCCTAAACCAGTTTCGTATTTTGGTACTACATGCATGTGGTAGTGAAATACTTCCTGCCCTGCCTTAGCCCCTTCATTGACAACATAGTTTAATCCAGCTGGTTGGAGCCGTTGTTGTAGCTTTAAAGCCATTTCTTTGGCGAGTAAACTAACCGCCTTCAAACTCTCATCATCAGTCGAGGAATAGTTAACGGCGTGTTTTTTGGGAATTACAAGCGTATGACCATCAGCAACTGGAAAGGCATCTAAAAAGGCAAAAGCGTGTTCGTTTTCACCAACCTTAAAGGATTTCACATTCCCCTCTACAATTCCACAGAAGATACAGTTATTGTTTGCCATACTTTGTTTTTGCACCATAATAACGGTTATTTATAACAAAAAGCCCCCACAGGATTAAAAACATTGATGCAAAAATAGGGATTGATCAAAACCAAACATAGCTGTTATATTTGGTTTGGGAGAAGAAACCATAGACCGAGTACGAATCATGCCCGTTGTCCCATATCTTTAGAAAAGGTACTGTTTGTAAATAAAAGGCATAAAGGCTTGGGTACACCCCTGCCTTGAGTAAAAACTTACCCAATTCATGTAGCGGCTCGCGCATTTTGTAAGTTTTAAAGAAGATTCAGATAAAGCCAAAGAAGCTAGCGGGGTAAATCACGTGGAGAAACAGCTGCGCAAATGAAATTCAATCAGTTAAAGGATTACCACTAAAACTGTATGGCATTTCACTCGTAATAGTCGAAAACCAACCCTTAACGTGTTCTTCTACATTAACAAATCCAGTAACACGTGACAGTACATACAGGTTAAAAAAGATGACGGTAAAGACCAAGTAACCTGCAGCACTTAAAAACAACGCATTATTGTCAAACAGCTTACAGCGTCGTTTACAGAGGTACATTAAGACACAAACAGCGGCAATTGTGTTGCTCTGGTAGGTAAAGGTATCAAAGTTAGTAAACCAGATTAATTGTGGTTGGTTGTTATAAACCACACTGATAACAGCACTAGCTACCATTACCCCAAGGTTAAACAGGGAAAACGGCGCGAGAAAAAGTAAATAAAAGTCGCAATTATTGTTAAGATAGAAAAAACGGTAAGCGAGTCCTATTGTTTTTTTCATCGCAAGTTAGTTAACTAATACATGAGTTTTAAAAAGATTACCGAAATGATGCGCCAAGCTGAACGACAAAGCAAGCAAAAAGCGCTTGATTTCGAGCAAAAATTGTTTGAGTACAGCTATAAGAATGCTGCCATCAAGATTATCATTTTTGGTAATCTCACCATTAAGTCTATCACAATCGATCCCGCATTAATTGACCCAGAAGACAAGGTCACTTTAGAGGAGATGATTACAGAAGCTGTCAATGAGGCTGTGGGTGATGTTAAGGCCAAGTACGACCAGTTAATGGAAGAGGCTATGCCACAGATGCCTGGGCTTTTTTAAGCTGCAGTGCTAGCTTAACCATTTGTTTATCTTTAGTGAGCTGTATAAAGGTTGGAATGTTGACGAGAACTGTCAACAATACAATGGCGAAATAGCTCAACCCAAAGCTAACTCCAAAGATTAAATCAGCATTTTTTGGATCTAGCTTGAGGAACACCGCTACATTCTTGTACTCACCGCTCAGTCAATCTTCAGCCAAAATGCCGGTAGTATTACCGGTAATTTGTCCCTTAAAGATGTTAACCATCAAGGCCACATAACTGAGATATGCTAGCGCAAACACGTGGATGTAGAAGAACGAAATTAAGCTAAAGCCATCGTCTCATAAAAAGACAGCCAGACTCAACAAGAAACTCAAGAAGTGCATCATGAAGTAAACCTGGTTGTTTTCCAAACCAACAAAGAGAAATTTGACAATCTCCTCTTGTTTTAATGGAGTAAAAATTAACTCGCCAAATAAGGTTATTGCAGCACCGTAAAAACCAAAGATCGCCAACACACCCGCCACCTTTTTCTGGCGTAAAAAGATAAAGATCGGGCCGATTAAGGCAAAGAAGGGGCAGAGATCTAACAAAAAGATCCTGCTCAACCTTAGTGAATCTAAAGGAACATTTGCAACAGCATTCTTAGCCTTGGCTGCAGTGCCGTAAATATCGGGAAAAATATAGATAATTTGCCGCCCGAAAAAGACATAGAGAAAGAGAATAGCTGCAAAAACTTGTAAACAATAAAACTGGATTTTTAATTTGCGAATTAATGGGGTAAATCCCAAGATAACTAGCACCCCGGCTACTGTTAGGATGACTACAAGATAGTTAAGCGACTGCATTAATTGCTTAAAATTAAGATAATAATTTTTTAAATTTGTCTTCGATGAGCGATCGTTTAAATGATCAAGCACAAAATCGCTTGCAAAAACTCCTAAACCTCAAACAAACTGGCAACGATCCCTACCTCATTACTAAGATAGAAAACACCCACTCAGCGGCTAGTTTTCAAAAAGCATTTGCCAACCAATCTGACGCTGAGTTAAAGCAGTACCAAGTGATCTTAACCGGTCGGATTATTGCGCTCCGGCAGACCTTTATTATTATCCAAGACTTTAGTGGGAAAATGCAGTTGTACATTAATAAAAAAACTGCCCCCGAACTGTTTGAATATTTCAATAATTACATTGATTTGGGTGATCAAATAGTAGCCACTGGCCATCCAATGATGACCAAAACAGGTGTTTTAACGCTGAACGTAGAGCGATTACAAATTGTCGCTAAATGCTTACAAACCCCACCTGAAAAATGACATGGGTTGACCGATCCAGAAGCGCGAGCACGAAAGCGTTACTTGGATTTGACCTACAACCGTGCGCAGGTCGAAGTCTTTTTGAAACGCACCCAGATTATTACAGCTATCCGTACCTTTTTAAATGAAGCGGGCTTTCTAGAAGTGGAAACACCCATTTTACAAGCCGTTTTAGGGGGTGCTAACGCCAAGCCCTTTAAAACACACTATAACGCCTTAAAGGGTGATTTTTACTTGCGGATTGCAAATGAAATAGCACTCAAAAAGCTCATTGTGGGCGGTTTGCCGAAAGTGTATGAAATGGGGCGGATGTTTCGTAACGAAGGGGTGGATACTACCCATAATCCGGAGTTTACGAGCATTGAAATGTATCAGGCTAACGCTGACTATGCGGTCATGATGGATTTAACCGAAAACCTCATTAAGTTTGTTTGCAAAACACTCAATCAGTGAAGTTTTAACTGGAACGGGCAAACACTTGATTTAGCCAAACCGTTCAAGAAAGTCAAAATGGTAGATTTAATCTGCCAAGTTACTGGGGTTAATTTTGATGATGTTAAGGATGATCAAACAGCCATTGCGCTTGCAAAGCAGCACAAAGTAGAGTTAAAAAAGCACGAGCAGAACAAACAACACATTATTAACCGTTTCTTTGAACAGTTCTGTGAGCATACCTTAATACAACCCACCTTTGTCACACACTATCCCAAGGCCGTTTCCCCGCTAGCTAAACAAGATCCCCACAACCCCGAGTTTACTGAGCGCTTTGAACTTTTTATTAATACAAAAGAGTTAGCGAACGCTTACAGTGAGTTGAATGATCCCCTAGAACAACGTGCGCGCTTTGAACAACAGTTGCAGGAAAAACGCATGGGTAATGATGAAGCAAGTGAATTAGATGAATCCTTTTTGGATGCTTTGAGTTTTGGTCTCCCGCCAACAGGCGGCCTTGGGATTGGGGTTGATCGGTTAGTGATGTTATTGTGCGAGTGCAGTTCCATCCGTGACGTTGTTTTCTTTCCCCAACTACGTGAATTGAAATAAGTTTGATTTCATTGTTTTAGGTGCTGGCATTAGTGGCATAGTTTTATCACATGTTTTAGCACAGCATGGTAAAAGTGTTTTACTGTTAGAAAAACGCAACCAATTAGGTGGTAATTGTTATGACAAGTTGGATGAAACCACCGGGTTACTGTTCCACCAGTACGGTCCTCATATCTTTCATACTGATAATCAGAAAGTAATGGATTTTATCCAACCCTTTTTTGAACTAAACAATTACCAGCACCGTGTTGGCTTACAACTGGACAACAATTTGGATTTAACGCTTCCATTTGACTTTAGCCAAATGCGCAAGTTGTTGGATACAAAAACAGCTAGCAGTTTAATTAACTTCTTTCAACAGCACTTTCCAGCTGAAAAGCACTTAACACTGATGCAGTTACAAACAATTAACTTTGCTCCAGTCCAACAGCTGTACCAGTTTTTAAAAATAAAGGTGTACGGTCCTTACAGTGTGAAAATGTGGGGCATGCCATTAGAACAAATTGATCCTAGTGTATTGGGTCGAGTCAAAATTAGTTTAAGTGAAAACAGTAGCTATTTCCCTACAGCCACTATTCAAGGTCTACCTAAGGGTGGTTATACAAAGGCCTTTACAAAGATGGTGGATCATCCTCTGATTGATTTAAGGTTAAACTGTCCAGCTAACTTAATTAGCGTAAACAATAATCAACTCTTGTTTGCAAATCAACCAATTACCAAACCAGTGGTTTATTGTGGTTTAATTGATCAGCTGTTTGGCTATTGTTTTGGGCGATTGCAGTACCGCTCGCTCCACTTTGAATGAAAGCGTTATGCTGTAAAACAACACCAAGCTTACCCCGTAATGAACTGGCCACTCCACCCAACAATAACGCGCCAAGTGGAATACAAACAGTTAACGCAAGAGGGTTTGGAGTCTAACCAAACGATTGTTTCGTGTGAAACTCCTGGTGCTTTTAGGGAAGGCGATCCCCGCTTTATGGAGCCGTACTACCCGCTAAATGATGTAAGTAATAATGCCTTGTTTGCGCGTTATTTAAAGTTAGCTAACGCTATTCCTAACATACATTTGTTAGGTCGGTTGGCACTGTACCAGTACATTGATATGGATAGAGCAATTGCTCAAAGCTTAGCTAAAGCCGAACAACTATTACAGTAATGGAACAACAAAAAATTAGGAACTTTTCAATTATTGCCCACATTGACCATGGTAAGTCAACGCTGTCAGATCGCTTAATTGAACGCAGCATTGGCTTTGAAAAACGCTTACTGCAAGCGCAAATGTTGGACACCATGGCGATTGAGCGTGAACGGGGTATTACTATTAAACTCAACGCCGTGCAGCTCAAAATGGCCCAAGGCAACCAGCAGTACTTGTTTCACCTAGTTGATACTCCGGGACACGTTGACTTTACTTACGAGGTATCACGCTCTTTAGCAGCTTGTGAGGGTGTTTTACTGTTAGTTGATGCTACCCAAGGGATTCAAGCACAAACGATTTCCAACACTTACTTAGCATTGGAAAACAACCTCGAAATTATTCCGGTAATTAACAAGGTGGACATGGAGAGTGCTGATGTCGAGAAAACCAAACAAGCCTTTCACCAACTGCTAGGGGTGGATCCCAACACCATCCCCTTAGTGTCCGCTAAAACCGGGCTTGGTATTGACCAGTTAATCACTACCATTATTGAAAAAGTACCCCCTCCAAAGGGCGATGAAAGTAAACCACTCAAAGCCTTACTGTTTGACAGCTATTACGATCCTTATAAGGGGGTAGTGTGCTTTATTCGAATCTTTGAGGGGAGTTTAAAACTCAATGATAAGATCCGCTTTGCTAGAAGTAACTCGGTTTACCAAATTGTGGAATTGGGAATTAAAAACCCCTTTTTTGAAAAACAAGACGTGTTAAAAGCCGGGGAGATTGGTTGGTTTTCCGCTGGGATTAAAAAACTAAGGGATGTAACGGTTGGTGACACGATTGTTCACGCTGAGGACACGACCACTCCACCCTTACCGGGTTACAAAAAGGTGTTACCGATGATTTACTGTGGTCTGTACCCGATTGACAACAATGATTACCAAAACCTCAAAATGGCCATGGAAAAGATTATTTTGAGCGATGTGGCCTTGGAGTATGAATATGAAACATCACAAGCATTGGGCTTTGGGGTGCGTTGTGGTTTTTTGGGGTTGTTGCACATGGATGTAATTAAGGAGCGGTTGGAGCGCGAGTACAACCTTAAGTTAATTTCCGCTCCTCCTTCAGTGCGGTATAAGGTGTTGTTAACCAACGGGGAGGAATTGGAACTCGATAACCCTTCACTCTTACCAGAGCGCTCGCGAATTAAATCAATTAGCGAACCGTTTGTACGCGTTTACATTGATCTACCCGATCATTATTTAGGTACAGTAATTGATCTGTGTCAAAACTTCCGTGGGCAATACGAAAAGTTAGAGGAAATTGACATTGACCGCAAACGCTTGGTCTACTTAATGCCCTTGGGTGAAATTATCTACAGTTTCTTTGATAAGTTGAAATCAATCACTAAGGGTTATGCTTCGCTCAATTACGAGTTTGACCAGTACCAGGTGTCCCAATTGGCTAAGGTGGAAATTATGTTAAACAAACAGAAGGTGGACGCTTTATCTTTTATTGCCCACCACGACTTTGCCTTTCAACGGGCCAAGAAGTTCTGTGTCAAATTAAAGGAACTAATTCCCAAGCACCTGTTTGAAATTCCAATTCAAGCCACAATTGGAAGTAAGGTAATTGCTAGAGAAACGATTAAAGCAGTGCGTAAAGATGTAACAGCAAAGCTGTATGGCGGGGATGTCACGCGGAAAAAGAAGCTGTTGGAAAAACAAAAGGAAGGGAAGAAAAGACTGAAAGCAATTGGTAGTGTGGAGCTCCCCCAAGAGCTGTTTAGTCATTTGCTCAAAGATGAAGATTAACTTATTTATAATTGATTACTGTTTAATTACTGATACCTTGTAAAGGTATTAACTAATTATTTTGATCTTTTATAAGTAATGATTAAGGATTTTGATCCTAGTGAGTTTGTCGGTAAAACTCCTACCAAGATTTTTGCCTTTGGTGGTATCCAAGAAGTTGGAAAAAACATGTATGGAATCGAGTACGATGATGAGATTATCATCATCGACTGCGGTATTAAGTTTGCTAGTGATGATCTACTAGGGATTGATGGTATTATCCCGAGCTTCGAATACCTCATTGAAAACCAAGCTAAGGTTAAAGCACTGTTTATCACCCACGGCCATGAAGACCACATTGGTGGGGTGCCTTACTTGTTAAAACAAGTCGATGTACCGGTGATTTACGCACCCCGGATTGCCGCTTCTTTGATCTTAAAGAAGGTCAACGAACACAAGGATGCTAAGCTCAATAAAGTGGTCGTATATGATGACTTTAGCAACTTTGAAACCAAGCACTTCAAGATTGACTTTTACCGCGTCAACCACTCGATTCCTGACGCCTTTGGTGTCTGTGTTCAAACTCCCAACGGTAACATTGTTGAGAGTGGTGACTTTCGCTTTGACTTTGCCGCTGGCGGGGAAATGCTCGATGTGCATAAGGTCGTGAAGATTGCCGAGCGCAATGTCCATGTCTTTATGTGTGAGACTACTAACGCCGAAATCCCGGGGTTCTCCCAGAGCGAAAAGTTAATTTACCGCAACATCAACAAGATTATTAAAGAAGCACGTGGTCGGGTCATTCTCACTACCTTTGCTTCCAACATTACCCGGATTAACGAAATTATTGAGATTGCTGTTAACAATAAACGGAAGGTCTGCTTGTTGGGTAAGTCGATGGATGTGAACGTCAACATCTCGCGTAAGATTGGCTTAATGGACATTGACAGCAATGACATTGTGGAAGTCCGTGACATTAAGAATTACCCCGACCGCAGTATCTTAATCTTGTGTACGGGTTCCCAAGGTGAAGACTCAGCAGCCTTAAACACAATGGCACGGGGTAAACATAACTGGGTTAGCTTAAAGTCCACTGACACCATTATTATGTCCTCCAACCCGATTCCGGGGAACTATGCAGCGGTGGAAAATCTCTTGAATGAGCTTTCGAAGTACGGGGTGACCATCTTTGAAAACTCACCGAACATGAAGCTACACGCCTCTGGTCACGCTACCCAGCAAGAGTTGCAGTTAATGTTAAACCTCGTGTTTCCAAGGTACTTAATTCCGATTCACGGGGAGTACAAGATGATGCGCACCATCAAAAACATAGCGCAAGAATGTGGCATTAACGGTGATGATGTTGGTTTGCTAGCTAACGGTCAGGTGATGTACCTAATTGATGGCAAGCTGTATTACTCAGGGGAAGTGATTAATGCTGACCCGATTTACATTGAAAGCCGCAATTCTTCTCCAGACTTAGCGCGGGTAATTAAACAACGCCAAATCTTGAGTCGGGAGGGAATGTTTGCTGTGATTGTAGTCTTTGACAAAAACAATAACATTTTAGGGATGCCTACTCTGATTACTCGGGGTTGTTTCTTTGCTCTAGATTCCAGTCCGTTAATGACTAAGATTACCCACTCAATTAAACGCGGTTTGGAAAACGTTATTCAAAACAAGCGTTTTAATACCCGTGAACAGATGATCAAGGAATTGAAACGAGTATGTAAGGAAACGGTGTCTTACTTCATTTGGAAGAACAAGAGTCGTAACCCGTTGATTTCAACCGTATTGTCTTGAGTTTAAACCTTAGTTTTAACTAATTCTCAATTGGACCCAATTATTGGAAATTAACACCTTTATAAGGCTGTTTTTGCCAGTAGTTGGGTTTTCTTTTGTTTGCTCTTGAGATCTAATTAATGCATACAAAAAGCCATTAATTGCCCAAAAATCAGATTCTTTTTACTTTCTTAAAAATATTTTTTTAAGATTTGCCATCTTTTTTCGCTTGGGAAGATGAAATTTAAGTATGGAACGGTTGTTTTGGGCAGTTTTTTAGGGCTTTCTGTGGTGTTAGCTGCCTGTGGTGCTCGTGGTAAATTTGACCAATTTGATGATGGCAAAATTAAGCTAGCATCATCCTTAACTTCCAAAGCAGCTGCCAATGCTTTACAAACAATAATAGAGAAGTACAACATTGTCAAAAGCGGCAAGGATTACCCCATTGAAATTACCCAAATTGCTGGTGGTTATGACGGCGGGCGGACTGATCTCCAAACCCGAGTTAGTGTTAAGGATAAAACGAACTTTTATAACCTTATTTTGAACTATCCAGATTTAGTTTCTGTGTTAGCGCGCAGTGGAATGGAACTGCTTTTTGACAAAGTTAATGTTGATAAATTAGAGCCGAACTTCTTAAAGTTTAATGAACAGATTAGTGGAGTCGCTAAAAGTGGTAACTATGGTATTCCCGTGTCCCTTTCAACTGACATTTTGGTCTTAAATGGTCCGGTGTTGCACTATATTTTGAACAGTGCGAAGAAAGAAGAGGCTAATTCTCAAGTTAAAAGTCAAATAAAAACATCACAAATTCAAGCTAAAGGTTCATTAACAATTGATACAGATGAAAATACCAAATCACTTTGAGAGAAAATTCAAAATTCAGCCAAAGCTAATGGTGAAACTAATCAAAAAGGACGAAAAGCTGCAAAATCAAATAAAACTGCATTAGTTCAACTTAAAAATGGCGCTGATACTACCACTAACGAAGAGAATAAAGATACCAAAACTAGTGATGATAAAGTTAAACAAACTTGAGGTGATTATGTAGAAAAGGATGATGGTCTTAAAAACTATACCTTTAGGGCTAGCGTGTTTGAAAACTGGCATGATTTCCTCGATTTTTCGACAAGGGTAGCTAAATCTTTTACTGAAAAGGTAAGCAACATTACCAATAAAAAGGGCACTGATATTCAGGGAGTGCTTGGTGTCGACAGTTCTCCTAATGTTTTATTTGCCTCTGTATTTGCTGCAGGTGGTGGTAATTATGAAAACTTCTTTTATAAGTTGAAAGATGGTCGGGCTGATTTTAGTAACTTTAAGAACAAGGGTAGTTCTTACCAAAACCTCCAAAGCGTTTTTAAAGACTAAAGTGAACTAATTAAAAGTAACGGTCTTTTTGTCAATAAAGGTGGTTCTTATTCTTCCAATTTCCAGAAGTTTCACCAATTAGCTTACAGTATTTCTTCCACTTCTGGCTTTAAGTATTCGTTTGCCGATAAGAACGCTAAGCGCTTGAAGTTTAGTGACGACAACACCTTCGTTGAATATCCTGATTTTACTCAGGAAATTAAGTCGCCAGACCAACAAAATGGTCAAAATACTGATGACAATAACAGTAAAAAAGAAGAAAACCTTTTAGGTACCTTTGAAGTTAAAGATTACTCGAAATCTAATAAAGGCGGGAAAGGAAAAAGTAAGGGTAAAGGCGGAAGCAGCAGTAATATGAAGACTATTTACATTTACAAAGGCTCTATTCCTGAAGATAAAAAGAAAGGTGATAATGCCATTTGAGTTACTGAGAAAAAAATTATTGATGCTTTAGAAAAAGCTGCTAATTCTAATGGTGAAAGTACACAATCTAATCCAAAAACCATAGCACTTCAAGCAGCTAAAAAAGAAACTAAAAAGAGCAATAAGAAAGAATCAAAAACGATTGGATATACAACTACTGGTTATGTCAATGCTGATGGTAAACATATTTTTGATACAGACAGAATTAACAATGAGAGGTTTGATCGTAAAATTATTATTGGTGCTACTCTAGAAACTTTAGACCAATCTAGTACACTCCAAAGTGGTGAAGCAATTGTCTTACCAGCACCTGGTAAATATAAGAGTAGTGATCAAAAAACGGTTATGACAGCGCAAGGCCCCAAGATAATCGGGATCCATGCTAATGCTAAAGAGAACGAAGAAACACAAAAATTTGTTAATTGGTTTTAAATACGCCAGAAACTTGAGAAAGTAAGAGGAATAAGAAAAACTCTAATGAAAAACAAACTGCAGCACAATACTTTGCTCAAGAGGCATCTTACATCTTGCCCTTAAAAGAAAACTTTGAAAAGGTTCAAATTAACGATAAAGGTGAGAAAAATGAAAATGACACAAAGGGCAATACTTATACCGCTAAAGCTTTAGAACTCTTTAAAAAAGTTTCTCAAAATGAAATCGTGAGTTACAGTGACCCAAGTGACTTTAGAAGTGGTAAGTTTCGTGATGCGATTGGCTCTAACTTTAACGCCATTATTAATTCCAAAGGAGACTTCAATAAATTTATTAGTAGTTTTACCGCAAGTTTAGGCTCAGACTTTTAGTCTTTTAAATACCCAATTGATTTATTTTTTAATCGATTAATTTATTTAATGGAAAAACAAGTAGATAAATTAGTGAAAACTTTAATTCATCAAAAAGCGTTTCAATAAATCCGGTTCTTAGAAATAAGGTTTTAATAAAAAAACATACCAGCAGTTAATGCTTCCAAATTAGCTGTGGGTATGTTTATTCTTGTCAAATAAATAAAAAAAGCCATCTATCTAAAAGACAGACAGCTAAAAAATACTTTTTTTAACGATATTGAAATTAACAGAAAGATCTGTTAGCAAATGTGAAGGAAGAGGCTCTATGCAAACTTTCAATATGTGGCGTGTTGAAAACCTTTAAGAATATGCACCAATTGGGGGCAGAAAAGCAGGTCTTCAACATAAATTTTCGTTTTCCTGCTCCCATTAGATAAATTGAGTTGCTTTTTAAAAGACGCCAAAAAGATTAAATGTTCTTCTAATTTGGTTTTTTAACTTCTTCTAACTATTAATAGGGTCTTTTACTTTCTAAAAATTTTTTGAAGTTATTTTGTCACATTTACTTTTTGGGCAATTTAGATCATGAATACCGAAAGAGTATAAGATTCATTAAGTTAACGAGGTATCACCTCTTTTTAAAACAAGCCCAATCGCAACTCGTTTCTTTGTTTAACCAAAACTTTTTGCAAGTTTTGTTGGCATTTCTAAATAAATCTTTCAATTTATTTAGTGATGACACAAAAATTAATATGTTGAACTTTTTAAATAAATCAACAATGGTATTGATCCCCGTTGGCAGCAGTGCTCCAAGTTCTAGTTCCTTTTTAAATCACCTCAAGAAAGAAGGTATTGCTTAATGAAAGAGAAAATTTCTAAAAAGGAATACAATGCCTTAATCCGTAAAACTGGCGAAAAGCACTTTGACGGCGAAAAGGAAGAGTACGGTGATGGCACCGTTGGACTTTGAACTTATGAATTGCGAAAATATAAACTTAAACCACCTGTGAAAGTTAAATACGTAACGCAAGAAGAGTTTGGCGAATTCAAAGATGCCACCAACCAGCGGCTCACAAAGATTGAGAACGCTTTGGTCGCTCAGGGCGAACAAATTAGAGCTCAGGGTGAGCAATTAAGTCAACTAATTAAAGTTGTGCTTCTTCAGGGCGAACAAATAAAATCTCAAGGTGAGCAAATAAAATCTTAGGGCGAACAAATCAACAAATTAACTCAAACTGTTGAAAAGCAGGGCGAGAAAATTGAAGTCCAAGGACAACAGATTCAAACAGTTAATGAAACTTTGAAGTTTGTAGTTGAAAGCCTTGAAACTATCCATAAACGGCTCGATGTAATGGAAATTCGTTTAGACAAACTCGAGTCCAAATAGATCTGATTCAAATAAGAGTACCAGCAGTTAGTTCTAAAAGCAAGCTGTTGGTATTTTTATTGATCTAAAACTACCTAAAAAAATTTTTTGCAATTTTGTTGTCACATATAAAGTCTTAGTTCAATTTATTTAGTGGAGGAGCAAAATGCAAATCTTTAATTTTTGAAATTGATCAGTAAAGAGTCTGATCCCTGTTGACAACCGAGTTGCAAGTGTTAGTTATTTTTTAAAAAATCAAAAGAAAGGAGGATTTAAACATGAAAAAAGTTAAAGTTCTAGAGAAGCCATCTGTTGAAAGAATGATAGAAGTTTTAAGCGACTCTGAAATATTGAAGTTTGATTTTTCTTGCTATTATTTACCTGATGGTACTAATGAGACGCTCATAAGAATCTTTTTTCAAGAAAAAGTTTCAAGTTCTGTAGTAACAAAAGATAAAAATTCTTTAAGACTTGATGAATACTTAAAAGACTTTATTAGCTTCTTAAAAACACAAAGAAAAAACTAAGAGCGTAAAAATCCAATAGTGAAAATTCACTGTTGGTATTTTTATTTATGTCAAATAGAAGATATTAGATGGAAAATTCAAATAGAAACTATAACACACAGAGCTAAGTAATTTTTATTTGTACTTTTTCTTGTTTAAAAAGCAGTTACTATTTATTCTTTCTTTATTTCATTTTTATCTTTGAAAAATGAGAAAGTTGACAATATTAAAAACTTAAACTTTTTTTAAGATTCTGTAGATTTTCTGGGTTTTAGTGCATGAAATTGAAATATGGAACCATTATTTTCAGTGGTCTTTTGGGAGTTTCCGCCATTTTAGCTGCCTGTGGTGCACGCGGCAAATTTGACCAAGTTGATGATGGCAAAATTGTTTTAGCTTCTTCTCTTACATCTAAGGGTGCAGCTAATGCCTTGCAAACAATTGTTAAAAAGTACAACGAAGTTAAAAATATTGATGACTATCCCATTGAAATTATCCAAATTGCCGGTGGTTATGATGGCGGTCGTGGTAACTTACAAACAAAACTAAGTGTTAAAGACAAAAATAGTTTCTATAACTTAATTTTGAACTACCCAGACGTAGTTTCTGTTTTAGGTCGAGTAGGCATGGAATTGCCCTTCGATAAGGTTAGAACAGATAAATTATCACCCCGTTTTCTTGATTTCAACAAGCGAATTAGTGCCATATCTAAGCAAGGTATTTACGGCATTCCAGTCTCTTTGTCTACTGAAGTTTTAGTTTTAAATGGTCCGGTATTGCACTATATTTTGAGCAGTGCAAAGGGTAGTAGTGGCAAAACGCAAGTAAGTCAAACATCATCAGGATCAAATCAACAAAAAACTCTTCAAAAACCCTTAAAGATTGATACAAGCGATTCAAGCACTTCTTCTCTTTGGACTCAAATTGAGAACGCCGCTAAAAATAACGGTAAAAAAGCAAATAATAGCAAAAGCAATAGAAGATCCACAGATCAAAGTACACAAACACATAACGATCAAGGCGATGCTTCTGAAAGTGATAAAAAGATTAAAGAATCTTGAGGCGATTATGAAGAAGTGGATGGTGGTTTAAAAGGCTTTACTTTTAAAGCTAGCATCTTTGATAATTGGCACGATTTACTCGATTTTTCTACTAGAGCGGCTAAATCTTTTAAAAAGATAAAAGATAACAATACTAAAAAAGGAACTGATATCCAAGGCATTCTTGGTGTAGATAGTTCAGCCAATTCCTTATTTACTTCTGTTTTTGCAGCTGGTAATGGTGACTATGACAACTTCTTTTACAAGGTTGCAAACGGTCGGGCTGACTTTAGTAACTTTAAGAATAGAGGTAGTTCCTTCCAAAACTTACAAAGTGTTTTTAATGATTACAAGGGTTTAATTGATCAAAACGGTCTCTTTGTTAATAAGGGCGGTTCTTACTCTTCTAACTTTCAAAAGTTTCACCAGTTAGCTTACAGTATCTCCTCTACCTCTGGTTTCTATTATTCATTTGCTGGTAATAGTGCTAAGCGCTTAAAGTTTGGTGATAATTCTTTCATTGAGTATCCACAATATACTGTTCCAATTAAAGCTCCTTCAAAGAATGGTGATGGAAATAGCACAAACAGCAATAGCGATCTTTTAGGCACTTTCACTTTAAGTAGTGTTAAAAAGTCAACAGATAAAAGTAAAAGTGATAGTCAACAAAATCAAGGCAAGAAAGTAGAAGGAACACCTAACCAGGGTAAAAAGGCAGAAGGTGCCCAGAACCAAGGCAAAAAAGAAAATAACAGTACAACTATTGAAATTTACAAGAACAAAATTCCTGATGGTAAAAATGCTGGGAAAGATGCCATCTTAATAAAAGATAACAATCTCATTAAACAGCTAGAAGATGCTGCTAAGAAAAACGGTGCAGAAAGTAATCAAAAACAAGGTGGAGAGAGTAATGTTCAGAAAGAACAAATTATTGGTTATACCACCACTGGTAACGTTCGTGAAGATGGTAATCACATTTTTAGAGTAGACAAAATTAATGACGAACAATACGATCGCAAAATTATTGTTGGCGTTACTGTGGAAACTTTAGAACAGTCTAGCACTTTGCAAAGCGAAGAAGCAATTGTTTTAGCAGCACCTGGTAAATATAAAAGCACTGATAAAAAGAAGGTAACAATTACCCAAGGTCCGAATATTATTGGTATCCATGCTAACGAAAAAGAAAATGCAGAAACCCAAAAGTTTGTTGACTGGTTTTTAAATACAGAAGTAGATTGACCCGCTAAGGAAAATTCTTCTAATAAACAAGATCAACAAAATTCAACCAAAAAACAAACCGCTGCTGAATTCTTTGTTGAATCCGCTTCTTATATTCTGCCTTTAAAGGAAATCTTCGAAAATAAAGAGAAAAAAGAAAATACAAGCAATAGTGATAAAAATAAGAGTAGTAGTCAAAGGAAAAATACTTACGCTGAGAAAGCGCTAGAACTTTTTCAACAAATAAGTAAAGATGAAATAGTTAGTTACAGCGATCCGAGTGATTTTAGAAGTGGTAAATTCCGCGATGGGATTGGTTCTAACTTTAATGCTGCTGTAAGCTCTAAAGCAGACTTTAATAAGTTTGTTAAAGGCTTCATCGCCACTTTGGGTAGTGAAATTTAAGCGAATTTGTGAGCTAACTAATCACAATAATTTTAGGTTAAAACAAAACCTTAAAAAGGTTTAGTGAAAGAAATTAAACTGTTACTATCGTGGAGATTAAAACTTACAAAATCAAAGATATTTGCGATATTAAACGCGGTAGAGTTATCAGCAAATTGGACATTAAAAAAGATCCAGGAGTTTTTCCTGTTTATTCGGCAGCTACTAATAACGATGGGGAGTTTGGTCGAATAAATTCTTATGACTTTGATGGTGAATATGTTACTTGAACAGCTGACGGTTATGGAGGGGCTGTTTTTTATCGTAATGGTAAATTTAGCATCACAAATCTTTGTGGTCTTTTGAAAGTAAAAAATAAAGAAATTAGTTCAAAATATCTTGCTCATATTTTGAAACTAGAAGCACCGAAATTCACAAACCGTGTTTTTAAAAATAGACCTAAGTTAACACATAAAACAATGGCCGAAATTCCCATTGATTTCCCACCCTTGAAAATTCAAGAGAAAATTGCTACTATCTTAGATACTTTTACCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGAGAGCGCAGGAAGCAGTATGCCTTCTATCGCGATTATCTGCTCAACCAAGAAAATATCCGAAAAATTTACGGAGCAAATATACCTTTCGAAACTTTTCAAATAAGGGATATTTGTGAAATAAATCGTGGTCGAGAAATAAATGAAAAATACCTTCGAGAAAATCCAGGTGAATTTCCAGTTTATTCCTCAGCAACAACAAATGGTGGTTTAATTGGAAAAATTAATGATTACGATTTTCATGGTGAATATGTTACTTGAACAACGGGCGGCGCACATGCCGGAAATGTCTTTTACCGTAATGAAAAGTTTAGTTGTTCACAAAATTGCGGATTGTTAGAAGTAAAGAACAAAAATAAATTTAGTTCCAAATTCTTATGTTTTGCACTAAAACTACAATCTAAAAAATTTGTGAATTATGCCTCTGCAATTCCGGTTCTAACAATAAAGAGAATTGCAGAAATCGAACTCTCCTTTCCACCTCTAGAAATTCAAGAAAAGATTGCTGATATTTTGTTTGCATTTGAAAAACTTTGCAACGATTTAACCGAGGGGATTCCTGCCGAAATTGAATTGCGTAAAAAGCAATTGGATTACTATCAGAACTTCTTATTTAATTGAGTTCAAAATAAGAAATTAGAAAGTCTCAAATCTCTTTAATAATAAAAGTTAGTTCAGCAAACCACAAACTGATCCCACTTCTCCCCCACGCTCACGCGCTTCTCCACCGGGTTCAACCTCGCGGGGTCGGTTCTCGACCAGGTGTTGGATTATATTCCCTGGATTGGGAATGGGCACAGATATGGGAATGACCACCGGGGTAGCAACAGTAGTACGAGTGGGGTAACAACCCAGGGACAACAATCGCAAAATGCATCCGGAACCGAACCCGCATCCACTTTTTCGAATGTCGGCGTTGGCCTCAAAGCGAATGTCCAGGGCACCCTGGGTGGCAGTCAGACGACGACTACGGGGAAAGATATTCCTAAATGACCCACCCTCGACCAAGCCAACCTCCAGCTCTGAACGGGGGCGGGGTGGCGGAATGATAAGGCTTCAAGTGGACAAAGTGACGAAAACCACACCAAGTTCACGAGCGCTACGGGCAGTGGGCAGCAGGGCAGTTCTTCAGGTACAACTAATTCCGCGGGCAATCCCGACTCGTTAAAGCAGGATAAGGTTGATAAAAGTGGTGATTCGGTAACGGTTGCAGAAGCCACTTCAGGGGACAACTTGACGAATTACACCAACCTCCCTCCCAACCTCACCCCCACCGCTGATTGACCGAACGCGCTGTCATTCACCAACAAGAACAACGCGCAGCGCGCCCAGCTCTTCCTCCGCGCCCTGTTGGGCAGCATCCCGGTGTTGGTGAATAAGAGTGGCCAAGATGATTCCAACAAGTTCCAAGCCACCGACCAAAAATGGTCTTACACCGAATTAAAGTCGGATCAAACCAAGCTCAACCTCCCCGCTTACGGGGAGGTGAATGGGTTGTTGAATCCGGCGTTGGTGGAGGTGTACGGTCTGAGTTCCACTCAAGGTTCTAGCACCGGAGCCGGTGGCGCTGGAGGTAACACCGGAGGCGACACCAATACCCAGACTTATGCACGACCCGGAATCGGCTTTAAATTACCCTCCACGGACTCGGAATCTTCCAAAGCCACCCTGATCACCCCCGGGTTGGCTTGAACAGCGCAAGATGTCGGTAACCTCGTTGTCAGTGGTACCAGCTTGAGCTTCCAGCTCGGCGGGTGGTTGGTTACCTTCACGGATTTTATCAAACCCCGGTCGGGTTACCTCGGGCTCCAGTTAACGGGCTTGGATGCAAATGACAGCGACCAAAGGGAGTTAATTTGAGCCCCCCCGGCCCTGAACCGCCTTTCGTGGCAGTTGGGTCAACCGCTTGGGCCGCGTGGAGAGTGTGTGGGATTTCAAGGGGGTGTGGGCGGATCAAGCTCGGTCCGACTCGCAAGCAGCTACAAGTACCACCACCGCAACGAAGGCTACCTTATCGGAGCACACCAATGCTTTGGCCTTTCAGGTGAGTTATACCGACCAGGATTCGTACAAGGCTTCCACTCAAAGCTCCGGCCAAAACCAAAACACCTCCCCCTACCTGCACTTGGTGCAGGGGAAAAAAGTAGGTTCCTCTGATAAGCTTTCAGACGATCTTAAAAAACTGTTGGACCCCAACGAGGTTCGCGCCAAGCTGCGCCAAAGCTTTGGTACAGAAAATTCTACCCAACCCCAACCTAAAGCCCTCAAAACAATCACACCGGTATTTGGGACGAATAGTGGTAACCTCGGCAGTGTGCTTGGTGGTGGGGGTACCACGCAGGGCTCAGGCTCAGGTCAATCTGGTGTGGATCTCTCCCCCGTTGAACGGGTGAGTGGTCACTAACTATTAATAGGAACTTTTCTTATTAATAGGAACTTTTACTTTCAAAAAATTTTTGAAGTTATTTTGGCACTTTCAAACTTTAAAGACCATTTAAAAGGCATAAAAGGATAGTTATGTTTTTAAGAAATATTTTGCGATTCAGTTCGCTTTTGGTTTTGTTTGGTTTTGAGTCTTTTCTTTTGAATGTTCAAAAATTCTTTGAATTAATTTTTGGCAACTTTTCCAATTTATGCAAATTTACATGATGTAAAACACTCACGATGATAGTTTTCAAAAAATATTTATTTTATTTAGATAGCCAAATAATCCAGTGATCTGTTGCTGCCCACCTCTTTAAACACTTTTACGTTTTTCCAATTAATAATAAAGGAGGTCTTCACCATTAAACGCACAATTTCACTTACTTTGTTTAAGAAAAGACTCAACAAAGATAAGATTAATAATTGTCATGTTTGGGAAGAAGAGTTACCTGATGGTAGCTATGACATGGGATTTAGTGACAATTTAAACCATATGGAAAAACGAAAAAGTGGTTATGTTACCCAAAAGCAGTTTAGCGAGTTCAAAGATGCCAATAATCAAAGGCTGATAAAGATTGAAACTACTTTAGCTACTCAAGGTGAACAATTAAATCAATTAATTAAAGTTGTTATTCTTCAGGGCGAACAAATTAAAGAACTTCAAGTGGAGCAAAAAGCTCAAGGAGAACAAATAAAAGCTAAAGGAGAACAAATAAAAGCTCAAAGCGAGCAAATAAAAACTCAAGGAGAAACGCTTAAATTGATCCTACAAGCACTTGGAGGAATAAATAAGCGCTTAGACAAAGTTGATCCGCCTAAATAGCTTTAGGCTAAAAAATAAAAGTGCCAGCAATTAATCTATAAAGGTTAGTTGCTGGTATTTTTATTCATTTGAAATAAATAAAAAAGCCATCTGCTCAAGGCGAAAAGCAGACAGCTCAAAAATTTTTTAATGATTTTGAAAACTAACGAAAAGTGTTAGTTTGAGTTGTAAGACAGATATGAATGTTTATTAATTCAAAAAAATGTTCGTTGCTTAATGAAAGATGTGCTGAAAACCGTAACGGTAAGATAGATTTGTAAAAAGAGTAAAGAACAAAACCATGTACGCAATAACTAAATTGATGGGAACAGAAAATCAGGCTTTCAACACAGAATTTTCGTTTTCCTGCTCCCAATAAATAAATTGATTTAGTTTTTTAAATGTGACGTCAAATTTCAATAAAAATCAAAATTTCAAACCAAAAACTATAACACACCAAGCTCATTGATTTTAATCTCCAGTTTTTTCTCATTTAAAAAGGATCAAAATTTGTTTTTAATAGTTTCGATTTTATGTCTTCTTTAATCACTGAATTTTTAGGAATTAAACTTGTTTAATCTTTATTAAGATTTCTCCAAATTTTGGGTTTTAGTGCATGAAATTTAAGTATTGTGCCATCTTTTTCAGTGGCTTTCTAGGTCTTTCTGCCATCTTAGCTGCTTGTGGAGCGCGGGGCAAATTTGATCAAGTTGATGATGGCAAGATTAAATTGGCTTTCTCTTTAACTTCAAAAAGTGCATCAAACGCCTTACAAGCAATTGTTAAAAAATATAACGAAGTTAAAAAACCTGGTGATTACCATATTGAAATTACCCAAATCGCTGGTGGTTATGATGGTGGTCGTAGCGATTTACAAACCCGAGTCAACGTTAAGGACACCACTAACTTTTACAACCTTATCTTGAATTATCCTGACTTAGTTTCTACTTTAGGTCGTGTTGGGATGGAACTACTGTTTGATAAAGTTAATACAGATAAATTATCCGACCGTTTCTTAGATTTCAATAAACGAATTAGTGCAATTTCTAAACCAGGGATTTACGGTATTCCGGTTTCTTTATCCACCGAAGTTTTATCGATCAACGGACCGGTGTTGCACTATATTTTGAAAAGTGCAAAAGGTGATAGTGACAATGTTAAAGTAAGTCAAAGGACTGGAGAAACTACTCAAAAAAGTAAAGTAACTAATCCTTTGAAAATTAATACTCAAAACGATCCTGCTACAAAAGATCTTTGAGAAAAAATAGAAGCTTCTGCAAAAGCTAATGGTAAAAGCAATAAGGATGGACAAACTAAGGGTAAAAAGAAAGTAGAAAAAAGTTCATCTTCTTCGTTAGTTAATTTAAAGCAGTCTACGGACCAAACAACGACAGATGACGGTTCTCAAAAAAGTGATAACAAAATCAAAGAATCTTGAGGCGAATACCAAGAAGTAGATGGTGGTCTTAAAAATTTTGAATTTAAAGCGAGCATCTTCGAAAATTGACATGACTTACTTGATTTTTCTACTAGAGTGGCTAAGTCGTTTACAAATGTAAAGGGAAAAGATATCAAAAAGGGAACTGATATCCAAGGTGTACTTGGTGTTGACAGTACGCCCAACTCATTATTCACCTCTGTTTTTGCAGCTGGTGGCGGTGATTACAATAATTTCTTCTACAAAATTGAAAACGGCCGTGCTGACTTTAGTAACTTTAAGAATAAAGGCACTTCCTACCAAAATCTTCAAAAAGTATTTGGTGATTTCAAAGGCTTAATTGACAAAAATGGTATCTTTGTGAATAAGGGTGGATCTTACTCATCAAACTTTCAAAAGTTCCATCAATTAGCTTACAGTATTTCCTCTACTTCCGGTTTTTTCTATTCGTTTGCCGGTAACAGCGCTAAACGCTTAAAGTTTGGTGATAACTTTATTGAATTTCCTCGTTTTACTCAGGAAATTAAAGCACCTTCTACTGAAAATGGCGGCCAAAGCAATCTTTTAGGAACCTTTGAAGTTAAAGATAGCAGTAAATCTAAGGAAGTGAAAAAAACTAATAGGAAAGAAGATGGTGCTCAAAATCAAGGTAAAAAAGAAAGCGATAAGAAAACCATTTACCTTTACAAAAGTCAAATTCCATCAGACAAAACAGAAGGCGAAAATGCCATCTTAATCAAAGAACAAAACGTTATTAATCAGCTAGAACAAGCAGCTAAAAAAGATGAAAAAGGAGAGACTGTTACAAACAAAGTTGCATCATTAGAAACCAAAGCTGCTAATGCCAAAAAGGATAGTAGTAAAACCATTATTGGTTACACCACAACTGATAATGTTCATGAAGACGGTAAAAACATTTTCAAAATTAACAAACTCAAAACTGAAGACTATGATCGTAAGATTATTGTCGGTGCCACTGAAGAAGTGTTAGAGCAATCTAGCACATTACAAAGTGATGAAGCAATTGTGTTACCAGCTCCTGGTAAGTATCAAAGTGGTGACGCTAAGAAAGTAACCATCACCCAAGGTCCTAATATCATTGGCATTCACGCTAACGAAAAGGAAAACGCCGAAACGCAAAAGTTTGTTAACTGGTTTTTAAACAATACAGAAAGTTGAGAAGTTAAAAATGGAAAAGATTCCTCCACTAAACAACAAACTGCTGCCGAATTCTTTGCTGAGTCAGCTTCTTATATCTTGCCTTTAAAAGAAATCTTTGACAAAGATAACAAAAAAGCTACTGAAAATAAGGACAATAATACTAGCAATAAAAAACAAGCTAATACCTATGCTGAAAAGGCGTTAGATTTGTTCCAACAAATTAGTAAAGGTGATATAGTTAGTTACAGCGACCCTAGCGATTTTAGAAGTGGTAAATTCCGCGATGGGATTGGCTCTAATTTTAACGCTGCAGTAAGTTCCAAAGCTGATTTTGACAGGTTTGTTAAAGGCTTCATCGCCACTTTGGGTAGTGAAATTTAAACGAATTTGTGAGCTAACTAATCACAATAATTTCAGGTTAAAACAAAACCTTAAAAATGTTTAATGAAAGAAATTAAATTGTTACTGTTGTGCAAATCAAAACTTACAAAATCAAAGATATTTGCGATATTAAACGCGGTAGAGTTATCAGCAAACTATATATCAAAAACAATCCAGGCGAATTCCCTGTTTATTCATCGGCTACAGTTAATAATGGTGAAATTGGCAGAATAAAAGATTGTGATCTTAAGGGTGAGTATGTGACTTGAACAACTGATGGCGCTCAAGCTGGTAGTGTTTTTTATCGTAACGGTCAATTTAACGCAACCAATGTTTGTGGAATTTTAAAGGTAAATAATGATGAAATTTACCCTAAATTTTTAGCCTATGCGCTTAGATTGAAAGCACCTAAATTTGTAAATTATGCTTGCCCTATTCCTAAATTAATGCAAGGAACTTTAGCAGAAATTGAGCTAGATTTCACAAGCAAGAAAATTCAAGAGAAAATTGCTACTATCTTAGATACTTTTACCGAGCTAAGCGCCGAGCTAAGCGCCGAGCTAAGCGCCGAGCTAAGAGAGCGAAAGAAGCAGTATGTCTTCTATAGCGATTATTTACTTAACCCAAAAAATTGAAAATAGGAAAATAAATATTACAAGCTGGGAGAAATAGCTCAAAAAGTTTTAGTAGGTGGTGAAAAACCTGCTGATTTTAGTAAGAAAAAGAACGAAGTTTATAAATATCCAATTCTTTCTAACAGCAGTAAAGCTGAGGAATTTTTGGTTTACAGTAAAACTTTTCGAGTTGAAGAAAAATCCATTACGGTAAGTGCTAGAGGCACAATTGGTGTCGTTTTCTATCGTGATTTTGCTTATTTGCCTGCTGTTAGTTTAATTTGCTTTGTCCCTAAAGAAGAATTTGATATAAGGTTCTTATTCCACGCATTAAGAGCCATTAAATTTAAAAAGCAAGGCTCTGCAACAGGCCAGTTAACAGTGGCACAATTTAAGGAATATGGTATTCATGTACCTTCGCTGAAAAAACAGAAAGAAATCGCCGCTATCTTAGATCCACTTTACAGCTTTTTTACTGATCTAAACGAAGGTCTTCCTGCTGAAATTGAATTGCGTAAAAAGCAATTGGATTACTATCAGAACTTTTTATTTAACTGAATTCAAAAACAAAAAGAACTAGTTGAACAAGCATCCACTAATTAACGGGATCTTTAAGGTAGAGTAGTTCTAAATTTTGGGGATTTTCCACTAGTTCAAACCGTTCGATGTTGTTTAGGAATAACTCCTCAATACTGTCGATGTTTTCAAAGTTTTCATATAACGGTAACTCGGTATTTGCTTTGCACAGCTTGACAAAATCTGGTTTAGCTAAGAGTGCTATTTCACTCGTAGTTTCGGTAAACAAACCACAGTAGTTCTTTTCATTGCCAGCACTAATTTTGCTAATGCCACAGCCATAAGGAATTTGAAGGCGGAGTGAGTTTAAAGCAAAGAGTTGACAGTTAGGGTTTACTGTGCACCAAGTTTTAGCAATAATGGCACCAACGCGTTGACCAGTAAAGCTACCCGGTCCAGTGGTTACGTAAAACTGTCTAACAGTGTTATTGCGTACTTTGTTTTTCTTTAACAATTTTTCCAAGTGATACACAGCCAACTCCGTGAGGTTTTGTTGTACTGGTATTGTTAGTTGATCCACCACAGTATTCGTTTTAAAATCCAACAGTACGATATTGAGGTGTTTGTAAGCACAGTCCAAAAAGAGCTTGTACTTATTGAAAAAGCGCATAAAGGGAATTAGCAATTTTCAAGTTTAAATTATCTAACTTATCGCTAAAAGTTTTATCTAACTGTGCTTCAAAACCCATTTCCTTATTCAAAGTGGGGTGGATAAAACTAATTTGTTGCGCGTGTAAATACTGGCCATATTCAGTAGCTTGCTCACTCTTCAAACCGTATAAGGGATCGTTGTAAACGGGGTGTTTAATAAACTGCAAGTGTACCCTAATTTGGTGAGTTCGTCCGGTTAACAGTTCACACTTAATTAAAGCAGCCTTTTCGTTTTGGTTTAACACCGTAAACTTAGTAAAAGCATTCTTGGCCTTGTTAGAACTGTTCCCCACCTTTCACATGACCTTATTGTTACCCACTCGTTCTAACGGGGCGCTAATGGTACCAGACAAACTGTCTAGGGGAAAATGGACTAAAGCTAAGTAATAACGCTTCATAACCCGTTGCTGGAGCTGTTTTTGCAAGTGCAGCAAGCTACTTTGGTTTTTCGCTAGCATAATCACACCACTCGTATCACGGTCTAGGCGGTGGACTAAAAACACCGGTTGGTGGTTGCAGTGAAACAAAGCGGCACCCAGTAAACTTTCACTTTCATTAAAACTAGTCGGGTGGCTAATTAAACCATTGGGTTTATTGACCACCATAATTTGCTCATCTTCATATAAGATGTCCAAGGGAAAGTCATAGGGCTGGACTTCAACTGGCACTTTTTCAAACAGTTCCTCACTCCAATTAACGTGTACCCGGTCTTCTGGTTGCACTAATCAACCATTTTTAGTAATTTTTTTACCGTTAACACTCACCAAACCATCGACAATTAACTTGGCTACTTTAACCCGGGAAAGGTTGAGTAGTGTGGCCAAAAAGGTATCTAAGCGTTGGGCTGTAGTAACACTAAAAGTTTGTTCCATTAAGATTTAGTTTGCTTCATTTCTTTGAAGAACTGAATTAAAAAGGAGAGGAACAGACCAATAAAACTAAAGGTAATGCAACAGTCCGCTAAGTTAAACACGGAATTACCTCCACTAAAGACAAAGTAATCTAGTACTTCACCACTACCCGAGGTTAACCGATCAAAGAAATTACCAAGTGAACCAAACGCTAAGGTAGTAATTCAAAAGATGTAGTTGTAACTAGTAGAGAAAACCAAAAAGAACAGTGCTATGAAAGATAGAAAACCCTGGAGAAAGTAAACAAGTCCTGGTTGGTCTGCCAATAAGCTAAAACCAACCCCCTTGTTACCGATAACATAGATACTAATAAAGGAACTGCGAGCTACATCTGTACCATTAGCACCATTAATACCCTTCTCACCATTTAAGGCAGCACGCAGCATAAATACCTGAAACAAAATGACAAAACCGACAAACAGGATTAAGGCAAGCTTATAGTACAAAAAGGGTTTGCGGTTAGCAAAAAGGATTTGCTTTAGCAATTTTGAAAAAAAAGTTGGGGCTTTTGCCATCTTGATGAGCTTAATTTTAAAAATGTAAAATAACGGCCATGGATTTGCTTACAACCGCAAACAAAACGCCGCGAATCGCCAAATCACGTCCTGTTCGAATTGCCATCATTATAACGCAGAAGACTAATGATCTGCACGCTACTGTACCCTGCTTTTTATGGCGCAAAGCAAGGTATGCTGTTGACCTGATTTCCGCTGAAGCCAAAGCTAGCATTATGTTAGAGATGGGGATTCATGTGCGCTGTGACAACACTTTGTCCAAAACTAACTTTAACCAGTATACCGCTGCTTTTATTCCCCATGGCAACACTACTAGGTTGGTGGAAATAGACAAACTAAGAAAGGACTTGGAGAAGTTTGTCTATAAACCCAAGGGACCAATGCGCTGGTTGTTTAGCAGTGGTAATGGTGCTTGTGTTTTGAAGGAGTTTGACTTGATTGCCCCTGACCAATTAGTCACCGTTCAAAACGAAAAGGAAATGGTTAAACTTTTGGGAAAAAATTTCATCAAGCAACCAGTACATGTGGACAAAAACATTATTAGTTGTGCCAATTCTTGTGGCTTGACTAAATTCTCCTTTAAGGTAATTGAAGAGCTTTCAGGAATAGAACTAGCGCGCAAAACGGCGAATCTTGTTGACCATATCTACAAAGGATAAAACCAACAATTTAAAATTACCTAACGCGCTTGCGCTTAAAAAATCGCTTTGAACAACACGAAAAACAAGTCAGATTGACAGCTCTTTTTGGAGGACTACCGTTTTTATCACGAAAAGGAATTTGATTGAATTACTTACCTCAATCACTGCCTCAACAGCTATCCTGACTTTGACATTTTAAAGTTCATTCGCAAATACGGTCCGGAGTGTGAAAAGAGCTTTTTATCACTCCAAAGTAAAACTAAGGCCGATGTTTATGGCGTGTTCACTAAGCAAATTAAAGCTGGCAGTGTTAACGAAGTGTTAGCGCAAAAATTAGTCCAACTCGATGCGCTGCGCACTAACTATCTCATTGGCGCTTTGTACTCCACCAATAAAACACAAAAGAAGCTGTTTAAGCAATCGTGAAAGAATGCTAAAAAGCAGGGTTACACAAAGCAAGAGTGGCTAATGACCTTAGTCGGTTTGCCGTTTGAAAAAGGGGAATATCATAAGCAACTGTATGCGCATTCCCGTCAAGAGATTTTGGATCTAGTGGAAGCGGTCAAAAAGCTGTATCTCAGACCAGAAAAGGATGACAAACTGGAGTTTGCTGATTCTTCCAAGGTAAGTGAGTCTAAGAGTATTAAGGTAACCAATGCAGTAACCTTACCAAGTGATGATTTAGATAAAGAGCTGTTTGAATTTAGCGGGGAAGGGGGTGATGAATAATGCCAAAGATTGAAGTTAAAAACGATGATTTAGAGTTAGCTTTAAAAAAGTTTAAACGGGTTTCGCTAGAAATTCGCCGTTTAGCACAGCGTCACGAATACCACCTTCGAAAAGGAATGCGCTTGCGTGAAAAGCGAAAGATTGCGCAGAAGAAACGCCGTAAGTTTCGTAATATGGTCTAGTTACTAGCACCTTACCAGTTTTATATGGATAACGATAAAAAGGATAAGATTATTTTGAGTGGTGAGTTAACTAACCATCGCTTTAATTTTACCAAGGATGGGGAAAACGGCTATAGTGCTTATGAAGTAGATCGTTTTCTAGATCAGCTAGTTCACACTTTAACCCATTACGAAGCGCAGCGTAACCGTGAGGAGGAGATGAAAACGGCTTACGAAAAGCTCTTTCAAGACCGCGACGAAATTTTAAAGCGTTGCTCCAAACTAGAAGCAGAGCTCAATAACTTTTATGAAAACGGTTATTCCAACCGTGTGTTAATTAGTCGGGTTCAAGCCTTGGAAAACAAGATCGAAAGCCTCCCTAGTGGTCAAAATGACCGTCTAGAGCGCATCGAAAAGTTGCTAAAAAGGGTGATTAAGCACTGGACTGACGGGGAGGATTTATCCTATGGTGACTTCGATGATGATTTCTTTTAAAAATGATTCTAGGCATAGGGATTGATTTAGTCGAAATCAAACGCTTTGAACAATTAGCTAGACAAACTGATAACTGTTTTGCGAAACGCTTGTTGACTTCAACGGAGTACGCGCACTATGCGAAGCTGCGTAAAGATAGTGAAAAGTCGAGTTTTTTAGCAGTCCACTGGTCATTAAAGGAAGCGATTTATAAGGCTGTTAACCACATCAAGCCATTGTTTAGCCAACTGGAAATTACAAAGAAAAACCAACGTTATAACTGCCAGATTGACCCTAAGATTGAACTTTTGCTCTCGGTTAGTTACAGCAGTAATAACATTACTGCCATCTGTTTGGCACAACAAACACCATGAAAAAATTAACACAAGCTTTTTTGCGTTTTTGTCTGAGATTCTTACAACTGCTCAGTTTAGTTTTGGTTTTACCCGTTTTTGTGTTAATGCTCATCTCTAGCCTAATTAGTGCTAAAAATTATGAAAGCATCCCGGAAAACTATCCCCCAGAAATCCGCTTTAAGAAGGTGTACCGCTTGGTATCACTTTTCCTCTACATTAAAGGAGTTAAAGTGGTTATTGTTAACCCTGAAAATGTGCCTAAAAAGGCTGTTTTAGTGGTAGCTAATCATAAGTCCAATTTAGATCCCCTCATCCTCATTAAGGCCTTTGGTAAAACTGAAGGGGTACCACCGTTAACCTTTATTGCCAAGATTGAACTGCAAGATACCTGACTGTTCAAGATTATGAAGCTGATTGACTGTGTCTTTATTGATCGAAAAAACCTGCGTCAAATGGCAGCATCCTTGGAACAACAGCAGCAAATTATCCGCCAGGGCACTGCTCTGTGTGTTTTTCCTGAGGGTACACGGGTACTCTCTAGGCAAATTGGTGAGTTTAAATCGGGCGCTTTAAAGGTCGCTTACAACGCCTTTGTGCCGATTGTGCCACTAACGATTGTGGGTAGCATGGGACACATGGAGTCCAAAAAGCGTTTACAAAAAGCCCAGGTAGAGCGCGATCGTGGTTATAAAATCCAGGTCATCTTTAACACACCTATTAACCCAATTAACTTTAACCAGATTGATTCGCAAAATGTGGCTAATAATGTCTGGCGTGAAATTAGTCAAACTTATGCGCAATATTGCCAAGACTAGATGATCACGTTAATCTGGTGCCAAGATAAACACTTCGGGATTGGGAGAGACAACACCATTCCCTGAAAGTTAACGGAAGCCAACCAACACTTCTATAACACTACTAAGAACCAAACGGTTGTAATGGGTTACAACACCTTTCAGGAGTTAGGTGACAAGCTAACTGATCACAACGTGGTGGTGTTGAGTAAAAAACACTTTGAAGAATTGCAAAACAACACCAACATTAAGGTGTTCAATTCAATTGAAAAGCTCTTGCAACACCACTTTAACCGTGATTTGTATGTGATTGGGGGCAAGCAAATCTTCCACCACTTTATTGAACTGGCTGATCGTTTAATTATTTCGGTTTTACCAGTGGACTTTAAGTGTAACCTTCGCTTAAAGTTGGGCTTGGACAGCTTTGAATTAATGCAAGAACAACAACACAGCCAATTTAAGGTGCAGTACTGACACAAAAAGCACCCCGAACGTTTATCGTTTAATGTTTTTTTGGAAGATTACAATGGGACTTTACCAAACTTGCTGGAACTTTTAATTGATAAGAAGTTCAACTTGCACCAGGTCGATATTGCCAAAATTACGACACAATACCTCCACTTAATTAACACTAACTTAAATAAACAAGCAATTGAACCAATTACCGACTACCTTGTCATTACTAGTCGAATTGTGGAGCAGAAGGCCAATAACTTACTGCAAATTAATGATATTGCCCTAGACTCTGACTTTTTAGACAACAAGCTGCGTGATAAACTAGTAGCACAGTTAGTGGAGTACAAACGTTACCGTGAATCACTCGATGACTTTGAGAAGCTAAGGATCAATCGCTTAGCTTACTTTTCCAAAGATAATGACTTTAACCGCTTTATCCAAACAGTTGACAAGAGTAATACAGAACCCGTGAAAATTGAGGACGAGCTACCCAATTATGTAAGTGTCTTAAAGTTGCATCACGCCATGAATAAGCTAATGCAGCGCTGAAGAGCCCAGTTTTTAGCGAACAAAAACATTTCAATTCAGGAGCTTTCAATTGAACAAGTCCAAGCGGAAATTTTGGCTACAATTAAGCAATTTGGCTATCATAGTGTTTCGTTAAAACGGGTACTGTTAAAGGTCAACCACCACATTTCCTTAATGTATTTCATTACCGCTTTTGTCGCTTTATTGGTGTTAATTAACAACCAGATTATCGACATTGAGCAGACTAGCTTTGATGATGAGCTTTACATCTGCTTGTTAGACAGTTCGCGGATTGAACAGCTCCAAGAAACGCCAGAAGCCATGGTGGAACGTGCTGTGAAACAACGCCAGGAAGCACAGGAATTAGCCCGTCAAGTAGCTAGAGAAAAAGCAATTGCCAACGCGCAGAAACGCGAAGCTTACCTAAAGGCAAAATATGGCAAGGATTACTTAACACGTGAACAGTTTTTAAAACTGTCTCCCGAAGAACGTGCTGCCCATGTGGCTAAAATGAAACAATTAAAACTAGTAAAGAACGACAATGGACGCGACAATTAAGGTAACTAAACCCGTTTTAAAACAAAAGGATAGCAGCGCTGCTAATTTAGTTGCTGCCATTTATGGGTTGTTGTTTGTAAGTGGCGAAAAGGGCTTAACGTTAGCGGAATTAAACCGCGTATTGCGCAAGGTGGGTTTGGAAAAGATTAAGGCCGCTTTAGTGCAGTTAGAGCGCAAGTTAAGTTTAGATGATGAAAGCGGGATTGAAATTAAAAAGTTTGGTCACAGCTTTCGCTTGGTCACCAAAATGGAGATCAAGGACTTTATCCACCGTTACCTACCCAACAAGATTAAAAATCCGCTCAATTCCAAAACAATGGAAGTGTTAGCAATTATTGCTTACAACCAACCCTGCACCCGTCCGCGCATTAATGAAATTAGGGGTGCAGATTCCTTTCAGATTGTCGATGACTTACTAGAAAAGGAGTTAATTGTTGAATTGGGCCGCAAAGACACACCTGGTCGTCCCTTTATTTATGAAGTGTCGCCTAAGTTCTATGATCTGTTTGGGATTAACAGCTTGGATGAACTGCCTAAAGTCGAGAATTTTGATCTCGACAAATTCCGCCAAGGTAGCTTTTTTGACTCTAACCGTTACGGGGATGATTAAGGTTATATAATTAGCGCAAATGAGTCCAAAAACAACCAAAAAAATTGCCATTTTAACCTCTGGTGGTGATGCTCCAGGGATGAATGCCACACTAGTTTACCTAACGCGTTATGCCACTAGTTCTGAAATTGAAGTTTTCTTTGTGAAAAACGGTTATTATGGGCTGTACCATGATGAGTTAGTACCCGCTCATCAATTAGATTTGTCAAACAGCTTGTTTAGTGCTGGCACCGTGATTGGTAGTAAGCGCTTTGTCGAATTTAAGGAGTTAAAGGTCCGCGAGCAAGCAGCACAGAATCTTAAAAAGCGCCAAATCGATTACCTAGTGGTTATTGGTGGTGATGGTAGTTACATGGGCGCTAAATTGCTAAGCGAGTTGGGCGTTAACTGTTACTGTTTGCCGGGTACGATTGACAACGACATAAATTCGAGTGAGTTTACAATTGGTTTTCTCACGGCTTTGGAGTCGATTAAGGTCAATGTCCAGGCCGTGTACCACACTACGAAATCACATGAACGGGTCGCCATTGTGGAGGTGATGGGTCGCCATTGTGGTGATTTAGCCATTTTTGGTGCCTTAGCCACCAATGCTGACTTTGTGGTGACTCCTAGCAATAAAATGGATTTAAAGCAGCTAGAAAGTGCTGTCAAAAAGATCTTGCAACACCAAAACCACTGTGTGGTGATTGTTAGTGAAAACATCTATGGCTTTGATGGTTATCCCTCACTTACAGCGATCAAACAGCACTTTGATGCTAACAACATGAAGTGTAACCTGGTGTCCTTGGGTCACACGCAACGCGGATTTGCCCCTACCAGCCTCGAGTTAGTCCAAATTAGTTTAATGGCGCAACACACGATTAACTTAATTGGACAAAACAAGGTTAACCAGGTCATTGGTAATAAGGCCAATGTGCCCGTTAACTATGACTTTGATCAAGCCTTTAACATGCCACCGGTTGATCGCAGCGCTTTAATTGCAGTCATTAATAAAAATATTATTTAACTGATATGATTCACCACCTAAAACGCACTAAGATTATTGCCACCTGTGGTCCGGCTTTAACTAAAAAGCTATGAACGCTAGCAATGTTAGATGACCCAGCTTACGCTGCGATGAAAGCAGAAGCTTACGCCAACATTGAAAATATTATTAAAAACGGCGTAACGGTAATCCGCTTAAACTTCTCCCATGGTAACCATGAAGAGCAGGCTGTAAGGATTAAGATTGTTCGTGATGTGGCTAAAAAGCTCAACCTCCCGGTGTCAATTATGTTAGACACCAACGGTCCGGAAATCCGCGTCTTTGAAACGGCCCCTGAAGGGTTAAAGATCCTCAAGGACAGTGAGGTGGTTATTAACACCACCACTAAGGAAGTGGCTAAAAATAACCAGTTTAGTGTGAGTGATGCTAGTGGCACTTACAACATGGTAAACGATGTTAAGGTGGGACAAAAAATCCTCGTTGATGATGGTAAGTTAAGCTTAGTCGTAAAGCGGATTGATACGAAGAACAACCAGGTTATTTGTGTTGCCCAAAATGACCACACTATCTTTACCAAAAAGCGCTTAAACCTCCCCAATGCCGACTACTCAATTCCCTTTTTAAGTGCTAAAGACCTACGCGACATTGACTTTGGTTTAACCCACCAAATTGACTACATTGCCGCTTCCTTTGTCAATACAACGGAAAACATCAAGCAACTGCGTGACTACCTCGCTTCTAAGAATGCCAAGCATGTCAAGCTTATTGCCAAAATTGAGTCCAACCACGCTTTAAACAACATTGATGGGATTATTAAAGCGAGTGATGGCATTATGGTAGCACGGGGTGATTTGGGCTTGGAGATCCCATATTACAAGGTGCCTTACTGACAACGGTACATGATAAAAGCGTGTCGTTTCTTTAACAAGCGTGTTATTACCGCAACTCAAATGTTGGACTCTTTGGAAAAAAACATCCAACCAACTCGCGCTGAAGTTACTGATGTGTACTTTGCCGTGGACCGCGGCAATGATGCCACCATGTTAAGTGGAGAAACCGCAAATGGGGCGTTTCCCCTTAATGCTGTGTATGTAATGAAGATGATTGATAAACAGTCCGAAACGTTCTTTGATTACCAGTACAACTTGAACTATTACATGGCCAACTCCAAGGCCCGTCACAGTGAGTTTTGAAAGCAGGTGGTGTTACCGTTAGCACAAAAAACAGCACCAAAACGCAAACTGATTAACAGTGACTTTAAATACGACTTTGTGGTGCACGCCACCAACAACCTTAATGAAATCTATGCCTTGTCCAACGCTCGCTTAGCGGCTGCGGTAATTATCTTGACCAATGACCCCCAAGTATACACCGGGCACGGCGTTGATTATGGCATCTTCCCTTACTTAATTGACCAAAAGCCGCAAAGCTTAAGTAAAGCTGAGTTTAAGAGCTTAGCTAATGTTGCCATTAAACACTACCAGCAGCATGGGGAAATTAGTCAATTAAAGCAGTGTTTGGGAGTATTTCACAATAAGATCATTAGCTTATAAGCGCTAAAAATTAATTAGAGTTTGTAAAATATTCTACACGGTGCCTTAGCCAAGTGGATTCAAGGCCTGGAGCTGCAACCTCCATATCGTCAGTTCGAATCTGACAGGCACCTCCATGCACATGAACAATAAATCCACTACAAGGTGAGAACCCTTGCATGTGCAAACGGGAAGTAGCTTAGTTTGGTAGAGCACTTGGTTTGGGACCAAGGGGTCGCAGGTTCAAATCCTGTCTTCCCGACCATAATGGCTGGATACCTCAGTTGGTTAGAGGACCCGGTTCATACCCGGGTTGTCGTGAGTTCGAATCTCACTCCAGCCACCAAAATCATTAAGGATCTATAGCTCAATGGTTAGAGCCCCCGACTCATAATCGGTCTGTTACAGGTTCGAGTCCTGTTAGATCCACCATCTGGTTGCTGTGTTAGAATAGTACTTGCCGCATTCACCTGGAGACTTACCCAAGCGGCTGAAGGGTTCGGTCTTGAAAACCGAGAGGTGCTGAAAAGCACGCGAGGGTTCGAATCCCTCAGTCTCCGCCAAACCACATTGATCGCGGGATAGAGCAGTCGGTTAGCTCGTCAGGCTCATAATCTGAAGGTCGGGGGTTCGAATCCCTCTCCCGCAACCACGGTTCCATGGTGTAGTGATAACATATCTCCCTGTCACGGAGGGGTTGCGGGTTTGATTCCCGTTGGAACCGCCACGGTCTTGTAGCTCAGTCGGTAGAGCAACGGTCTGAAGAACCGTGTGTCGGCAGTTCGATTCTGCCCGAGACCACCATAAAACTTTCTTACCTTACCTTTAAAACTAAGGGTAAGGTTTTTTATTGCTTACCACCTTTATTTAAGGCAGTACAACAGCTGTAAATTAGTTGCCCAATTCTTTTTGCAATCAAGTTCACTTTGCCTTTTTTTCTTGGGCTTTTGTGTGGACGTGTAACAATTAAAGCAGTAAGCTTAGCCTTATTACATGTAATATGAAGTACAACATCAACGTTCATTCCGAAATTGGTCAACTGCAAACCGTTTTAGTCCACACCCCTGGTAATGAAATTAGACGGATTTCACCAAGACGGTTGGATGATTTACTGTTTTCAGCAGTAATAGAACCCGATACTGCCATCCAGGAACACCAAACCTTTTGTCAGTTACTGCAAGAACAAAACATTGAAGTGGTGCAACTGACTGATTTAACAGCCACCACCTTTGACAAGGCAAATGCTACAGCGCAAAACCAGTTTATTGAAACTTGACTAGATCAAGCAGAGCCCAAGTTAACCCCAGAACACCGTAAAGTAGCGAAGCAGTACTTACTGGAACAAAAAGCAAAATCGACCCTCAGCATGGTACGCTCGATGATGGGTGGGATTGATAAGCGTAAGGTAGCTGCTGCTAACACCATTAACGGTGATTTTTTAGTAGATCCGATGCCTAACCTTTACTTTACCCGCGATCCGTTTGCCTCGATTGGCCATGGTATTAGTATTAACCGGATGAAGTATTTAACGCGGCGGCGTGAAACCTTATTTGCTAGTTTTATTTTGCCAACCACCCCGATTATTGCCGCACGCAAATTTACTTTAAACCAATTGACATGGGCACAATTGAGGGTGGTGATATTTTTGTATATGACCAACAAACAGTGGTGATGGGGTTGAGTGAACGGACAACCGAAGCTGCCATTAATGTTTTAGCGAAAAAGATTCAACAAGACAGTAGCACTTCCTTTAAGCGGATCTTTGTCATTAACGTTCCACAACTACCGAACTTAATGCATTTAGACACGTGGCTGACAATGTTAGACCGCAACAAGTTCTTGTACTCCCCGAACATGTTGGCGGTTTTAAAGGCGTGGCGGATTGACTTTACTGATCCGGCTTTAAAGTGAAATGAAATAGCTGGTGATCTTTCAACGATCTTACACACCATTATTGGGCAAAAACCTATGCTCATTCCCATTGCCGGAGCGGATGCTAACCAAACCGAAATTGACATTGAAACCCACTTTGATGGCACCAACTACCTCACCATCGCTCCATCGGTAGTAGTGGGGTATGCGCGCAATAAATTAACACACCAGACCTTGGAAGCGGCTGGGGTTAAAGTAATTGCCTTTAAGGGGAACCAGCTGTCTTTGGGAATGGGTTCTGCACGTTGTATGTCGATGCCATTAGTACGCAAACCACTTTAATTTTTCTTTATTTACTTATACAAATTTATGCCAATTAACTTAAAAGGTCGTAGCCTTGACTCTGCTTTAAACTTTACTACTGCTCAAATTAACTATTTAATTGACCTCGCCATTGATTTAAAACGCAGTAAATACCAAGTTGCACATCCAGAACCGTCCCTTAGCGGGGAAGAACATTGTTTTACTGTTCCAAAAAGATTCTACCCGTACCCGTTGTGCCTTTGAAGTGGCTGCCTTTGACTTGGGTATGGGTTGCACTTACATCGGCCCTTCTGGTTCCAATTTTGGTAAGAAGGAATCGATTGAAGACACTGCTAAAGTGTTGGGTTCAATGTATGACGGAATTGAATTCCGTGGCTTTAAGCAAAGCGATGTTGATGCTTTGGTCAAGTACTCTGGTGTTCCTGTTTGAAACGGTTTGACTGATGCGGAACACCCTACCCAAATGCTAGCGGATTACATGACGATTAAGGAGTTGAAGGGTGATTTGAAGGGGCGCAAAATTGTCTTTGCTGGTGATATTAAAAATAATGTGGCACGCTCGTTGATGATTGGGGCAGCTTTTGTTGGTATGGACATAGTGCTAGTAGGTCCTAAAGCACAGCATGAATTAGTTCAAAATGGTGCTGGGTATAAAGAGGTCTTTGAGCAGTGTCAAGCATTGTTTCAACTCAATGGTGGTAGTGTTAGCTTTAGCACTGATAAGCTGCAAGCGGCTAAGAATGCTGATGTAATTTACACCGATGTGTGACTGTCGTTGGGTGAAGACTTTAGCTTATTTGAAGAACGGATTCAGGAGCTGGGTCAATTTCAAGTTGACATGGCTATGATCAAAGCAGCTAAGAGTGATGTGATCTTCTTGCACTGTCTCCCCGCTTTCCATGATGATCACACTAGCTTTTCACTGGAAATTAAACAAAAGTTAGGTAAAAAATATCCGGTGGTGAAAACGGGTGCGATGGAAGTGACTGATACGGTGTTTCAGTCCAAGCATAACATGGCCTTTATCCAAGCAGAAAACCGCTTGCACACGATTAAGGCCGTTATTTTAGCTACTTTAGGGTATTAAAGATGCAAAAAATTGTCATAGCATTGGGTGGTAATGCATTAGGTAACAATCCCACCGAACAAAAGGACTTGGTACAACTACCAGCACGACAGGCGGTTGCTTTATTAAAGCAGGGTTATCAGATATTATTGGGTCATGGTAACGGTCCACAGGTTGGCATGATCTACAACGCCTTTAGTGCGGCCAAACAAGTCAACCCTAATACACCCACTGTACCATTTGCTGAAAGTGGGGCAATGTCTCAGGGTTACATTGGACTGCATTTGTTAACAGCATTGTACAATGAGCTGCTACACCAAAAACTACCCCATCAAGCCGTGTACTTTTTAACACAAACCTTAGTAGAAGCGAGCGATCCTGCTTTTCAAAACCCAAATAAACCAGTTGGTCCGTTTTACAACACAGAGGAAACAGCACGCTCAGCTAATCCAAACAGCACCGTGGTGGAAGATGCAGGGCGGGGTTGGCGTAAAGTAGTAGCGTCCCCCAAACCAGTTGATGTTTTGGGAATTGATGCCATTAAAAGTAGCTTTAACCAAGGCAACTTGGTGATTGTTGGTGGCGGTGGCGGTGTACCCACAATTAAGACCAAAAGTGGTTATGCAACGGTTGATGGGGTGATTGATAAGGATCTAGCATTGAGTGAAATTGCCATTAAGGTAGAGGCTGACCTGTTTGTTATTCTCACTGCCGTGGACTTTGTCTACATTAACTATGGTCAACCAAACGAGCAAAAGCTAACCTGCATTAACACCAAGGAAGCTAAGACCTTAATGGCCGCAAACCAGTTTGCTAAAGGTAGTATGCTCCCTAAGGTAGAAGCGTGTTTAAACTTTGTCCAGTCGGGTACCAATAAGACGGCCATTATTGCCCAATTAGACCAATTAGCTGCGGCCATTGCGGGCAAGATTGGTACTAAAATTGTCAAGTAGTTATGAAACAACAAAAACCCAAAATTAGCTTTATAGCAGCGATGCTAATTGTTATCGGCTCGTCCATAGGTGCTGGTATCTTTTTTAAATCCAGCACTGTTTTGGAAAATTCCCAGGCCTCTTTAGTGCTAGCGATTTTTAACTGGTTGGTAGCTTCAGTGGCAGTGATTGCTATGGCCTTGGCATTAATTGAAATTGCCAGTGTTCGCAACGATAATCTCTCGATTATTAGCTGGGTCAAAGTGTTTAACCGGCGCTGGTTGTACCATGGTTGTAAGAATTTTATGACCTATTTGTACCTACCGTTGACCTTCTTCTTTATGCCGCTGTACTTTATCTGTTCCATTCAAGATGGGTTTCGCGGTTTGTTGGGACTGGAAACCGGAGCGCATTTCAATACTAGTGTGGACTGGTTAATCTGGTTAGCTTTAGCACTTATTATTACCACTTACTTCTTGACAATTCCACCACTGTACGCTAAGGTAGGGAACATCCAAAACATGGTGGTAAGCGCCGTGAAGTTTATTCCTTTAGTGTTTGTGCCTATCATCGGTTTTATTGTAGCGGGTACAGGTAACGGTGAATTAAAAAACGTTAAGGCATTAGTGCAACCCCCACAAATTAATGGTGCGACTGCTTCCTTTACCCAATTAGTACAAGCGGGGTATGGAATTACTCGTTTTACGGGAATTGGTGCTGGGATGGGTTCCTTTATTTCGATTGCCGCCATTTTCTTTGCGTACGATGGTTTTTACGTAACAGCTGGACTGCAGTCAGAGATGCGTGAACCGAAAAAAACACCGTGAGCTTTGTTTTTAGGTTTACTAATTACTACGCTGTTTTACCTTATCTTAGCAGTGGCACTTTCCATTAACGGTGGTTTGTTTAGTGGTATGGAAGAGTCCATGGGAAAGCTGTTTAACAATAAGCGTGCCGGGCAAATTGTCTTTGGTGTTGTTAACCTAATGATTGGCATAGGTGTCTTGGGTATTATTAACGGTTTTGCACTGTGAGCACCACGGTTTGTGGAAGACTTATTAGCCCAAGGTGATTTACCTTTTTGAAAGCAAGTGCAGGGCAGATTGAACCCCAACAAGCCAGTGGTGGGGGTAATTTACTGTTTGGTACTTTCTTTGACTGTTCAAGTGCTGTTTACGGTAATAGGTGCTTTGGCTTACCTCCCGACAGTAGCGGATTACAAAAATTACGTTAACACTGAAATTGACAAGCTCAATTCGATGCAATGGTTGTATTCGTTTTCCGATTTAATGGCTACTTGAACTTCGCTGTTTACCTTCGCCTTTATTGCCTGTGCCATCTTTGGTGCCATTGTTAATCGCAAAACCAAGAAGATTACGATTGCCAATCCAAAGCGTTATTTTCTCCCAGCGGCTTGGATTGCCGTAGTGGTTAACTGCATTAGTGTTTTTGTAACTATCATTGAGCCCTTCATTAACCTCTTTTTACTGTTTGGTTATGATGAAACAGTGGCACACACAGTGCTGGGTAATGACTTCATTGAATTGAATGAATTAGTGATAGGACGGGTAATGCTGATAGTAGTGTTAGTTTTCTTTGCCATAATTAGCTTTCTCCCTGTTTATGTGGAGGATCAATACCACAAACGTAAGTTTGGTAGTTTAGCTAACTATCAGCAATATGTACAACAACATTTAGCACACTCCACCATTAACGGTTAATTAGTTTTGTAAGGCTTGCAAGCTTTGCCGTCATTTTCATTTAAATAGCATTTATTCATTTGCATTTTTTTAGATAAATTAAAATTAATGGTATAGTGTCAGCTTTGCTGTTGGCACCTTTTAAAGACAGTAAATGGATATAAATAAACCAGGTTGAAATCAGTCTGACCAACAAGCTACTGCATACGATCCCAATCAGCAGCAGTACTATGGTGATGGTAGTACTTATTACGACCCCGACCAAGCAGTTGATCCCAACCAAGCGTATTACCCCGATCCGAATACCTATCCTGATGCAGCGGCTTATTACGGCTATGGTCAGGATGGTCAAGCATATCCACAAGACTATGCTCAAGATCCCAACCAAGCGTATTATGCCGATCCCAATGCTTATCAGGACCCAAACGCTTACACCGATCCTAACGCCTATGTAGATCCCAATGCTTATCAGGACCCAAACGCTTATGTAGATCCTAATAACTACACCGATCCCAATGCTTATTACGGCTATGGTCAGGATGGTCAAGCATATCCACAAGACTATGCTCAAGATCCCAACCAAGCGTATTATGCCGATCCCAATGCTTATCAGGACCCAAACGCTTACACCGATCCTTACTATGTTACTAGTACCGATCCCAACGCTTACTATGGTCAGGTCGATAATGTACCTGCTTTAGAGGCGAGTGATCTCGCTTATGAGGTAACACCGCAAGAGCAAGCAGCGGAACAAGAGCTGTTTAGCGAACCGGAAACAAAGGTAATCCGTGAAATCCACGAATTTCCCTTTGAGAAGATTCGCTCTTACTTCCAAACGGACTTTGACAGTTATAACAGCCGTTTAACCCAGCTCAAAGACAAGTTAGATAACGCCATCTTTTCGATGCGCAAAGCGATTGATACTGTCAAGGAAAACAGCGCTAACTTGCAAATTATGAAGCAGAACTTTGAACGCCAACTCAAAGAACAGCAAACCCAACGGCTTACAAGTAATACTGACGCGGAAAAGATTGGTGCTAAGATCAACCAATTAGAGGAACGCATGCAACGTTTATCGCGCACTATGGAAAGTGTGGAGTGAACCAAAAAGGAGCCACGTCAAGAACAGTTCGATCCACGCTTTGTCGATCCACGTAACTTTAATAACTATGTAAACAACACTGACACAATGATGTCAATGTTTGAAAAGGTATTAATGATGAACTTACTGCGCAGCACCACTCCAGTCCAACCTCCAGTGCAGTACTTTACTCCTCAACCCCTTACAGCATCGCCACGTCCAGTGTATGAAGAACCGATCTCCGCTTCGTTCCGCCGTCGTGGTTACCGTGGTGATGAATTTTACGAATAAACACTGGCTCTTTTAACCGATGAATGATACTGACAAGAAGTTCCCCTTGCAACCAGTTTATGACACTGGGTTTGATGATGGGTATTTACAGCGTGATTATGAAAAGTGTTTAGAAAGTGCTGCTGCTAATGATGCACAAACAGTGGAACTACAAACCCAGCTGTTAGCAGAGATTAAAAACCTTGAAAACGAAATCAAGGCCCTAAAGGCGCAGGAATCACGCCAACCCGATCCGCACAACAATGCCCGGATTCAAAGCTTAGAAGCTAGCTTAAACCGTTTGGTCAATGAGTACAACAACTTTGAATTCCAAAAGAACTATATGGTTGATCGGGTAGCAGAGTTAAATAACAAAGCGCGTTTTTTTAAGGACGAATTAAAGCGTCTCCAACAAGAAAACGCCGCTTTTGTTAATTCCCGTTATGCCAATTGAGCTGACTTTCAATCCAACTATCAGCTCAAATTAGACCAGTTTCAAGCGTTAATTGACCAGCAAAACCAAACAATTAAGCAGCTCAACGAACAGATTGCAGCTAACCAGGGCTTAATTGATCAAAACGTACAGCGCTTACAGCAAAACCACAGTTTAGACCAACAAGAACGTGACGCCTTACTGTATGAGGTTGACCATTTATACAACGAACTGTACGAACTTGAAAACCAAAAACGCTTAGTGGGCATCGAGTATGAAGCCACATACCAGGACTTGGTGAGTGCGGATGCGGAGTTGCAAAACGTTTATGAAACAATTGCCCAAAACCAGGCCAACTTCCAAAAGCAGTGTGATGCTTATTGAGCACAGCTCAAGCAAGTAGAACAGCAAATTCAAACGACAAAGCAAGAATTGGTTGATGAAGAATCGACACTTAAGGTGCGTTTAAATGACGCTGATTTTTACATTAACTCACGCTTAGCAGAATTAGATGATTTAACTAGCAAGATTAACGAACGTGATTTTGTTTCTAAAGAACAAGCTCAAGACGTTAAAGCTAGTTTAGCTAACCTCACTAAGGAAAAAGAACGTTTGAGCGCTGAGAAGGATAGCTTTGAAAGGTTAAGGAACACCGCTTTAAACGACATTAACCGAATGGAGCAGGAAAACGCGTTGTTTGCTAAACACTTAGAACAGCAACAATACGAGTTTGAAAGAAAGCAACAGGAAAGCTTATTAAAGCTAGAAACAGAGCATAAACAGCTCCAAAAACGAATTGGTGAGTTCAAAATCGAGAGTGAAGCTAAAAGTGAAGCGTTACTGATACAAGAACGTGAACTGTTGGAAAAACGCCGGGAAATTGATGATTTGTTAACCCAAGCCAGCTTGGAGTATGAACAACAACGCCGGACGAATCAGGTCTTAAAGGAAAAGCACCGTCAAGTCCAACAACACTTCCAAAACCTCGTGCATGCTAAAAAGAAATTGGATCAAAAGCGCCATTATTTAGCGGAACAAAAGCGGATTGATGAAGAACAGATTTTTAAGTTAAAGGAAAAGATTGCCACTGAACGCCGCGAATTGGAGAAGCTTTATCTAGTTAAAAAACAAAAGCAAGACCAAAAGGAAAACGATTTACTGATTTTTGAAAAACAGTTAAGGCAGTACCAAGCTGACTTTGAAAACGAAATAGAAGAGAAGCAAAACGAGCTGTTTGCATCCCAAAAATCACTCCAAAAATCGTTTACACAACTCAAAAATAAGGAAGCGGAATTAAACCAAAAGGCACAAAAGATTGCAGAGGATTGAGCACATCTCAAACAAAACAAACACCACCACGCTGATCTAGAAATCTTTCTGGAGGGTGAATTTAACCACTTACAACAAGAAAAGCACAAGCTGTTGGAAGCACGTACGCAGTTTGACAACCGTGTCAGTTTGCTCTCAGCACGCTTTAAACAAAAACAAGCAGAATTAGTTAAACAAAAACAAAGCTTGGAACAACTCACCGCTGCCTTTAATAAAGAGCAGGAAGCGGTAGAGCGTGATTGGAAGGATCGCTTAGCTAACTTAGAAAAGCAAAAGGAAATGCTGGGGGATAAGGTACACCAGTTTGATGAAAATTCGCTCAATATTTCCAAAAAGCTAGCGGAGCGGGAGCTAGCGATTAAATTTAAAGAAAAGGAGCTGGAGGCTGCACAAAAACAGCTAAGTTTAGACAACAACAATAACGCTGGGTTAAAGTTGCAGTTAGATAAATTGAGCGAATCACTCAAAACGGAACGCCTGGAATTAGAAGCATCTAAAGAACGTATTTTGGATTTTTACGATGAATCATCACGCCGAATTGCAGATTATGAAAGCGATTTACAAGCACGCTTAGCTGAGGTTAAGACACTAGAAAAAAACCAGCAGGAAACAGCGGCTAAAAGTGAGCGCGAACTCAAAGTAGCCTTAGAAAAGTTAAACCAAGCAAAAAAGGCTTTTTTACAAATTCGCAAACAGCAATTGCTCGAAATTGCTTCTGTAAAACAACAACTAGCACAAAAAGCTAATTTGTTGAAAAACCAACAAGCAGAGCTAGATAAGCAAACAGAAGAGTTAGAAGCAGCTTTTTTAGAGCAAGATACGGACAAAAAGGAGTTGGAAAAAGCACTCCACAGCGTTAAGTCCAAGCAAGAATTATTAGAGCGCGAACGCAGTTTTTTACTGCAAAAACAACGTGAATTTGCCGAACATGTAGCCGGCTTTAAGCGCCAAGTGCACTTTAAAACAACGCAAATGCAGCGTTTAAGTGAGTTCAACAAACAACAACAAAGTGAACAGATAAAGCGCGAAACGGAGCTCAAAATTGCTTTTGCTGATCTAAAAAAGGATTACCAGCTGTTTGAATTGCAAAAAAACCAGGAGTTCCAGCAAATCGAACAAAAGCACAAAGAACTAGAACTATTAGCACAAAAACAGGCCGAACTCAAACAGGAGTTAGAGCAAAAAGCAACAGCTTTAGCTAGCCAAGATCAAGACACAGTACAGGCCAAGCTAGATTTAGCGCGTCAACAGCATGAATTGGAATTGAGGCAAAATGCCTTTAACCAAGCTAGTCTTTCGCTCAACAAACAACGCGAACAGCTCACAAACCAAGTCAAGGTATTGCACGGTGAGTTGAAAAAACGCCATGAAAAGCTAACTTTAAAGGATCGTTTACTAGCTGAAAAGGAAAAGGATCAGCACAAAAAAGATGCAGAAATTAACCAACGCTTTAAGCAGTTTGAAAATGAATACGCTGATTTTGACCAAGCCAAAAAGCGCGAATTGCAAGAGTTAAACCAAATCCGTCGAAACTTGGAGCAGAGCAATGCATCACTGCTAAAGAAGCGCAACCAACTAACGCTTGATTTTGCTTTACTACGTAAAGTGCAACACAATACGCAAACTAACCGTGTCCAACTCAACACGCAAATTAAGGAGTTTTTACTAGAGAAGAAGAACTTTCAAAAGGCGAGTGATGAAGCCGCTTTACAAAAAGCACTGTTAATTAAACGCTTACGTAGTTTTGCTTCTAAATTACAGTTGCAACGCGAAGCTTTAGCGATTCAAAAACTGGAATTTGATAAGCGTGATGAACAACAAAAGAGTGAGATTAACAACGCTAAGTTACAGTTAGAGCAGTTCAAACTAGAAAAGCAGAACTTTGATGAAGCGAAACAAAAGCAGTTAATTGAGTTTAAGGATCAGTGCCAACGACTTGATGTTGAAAAACGTCTGTTAAAGCAAAAGTTAGTGCAGCTCAAAAACCTATCTAAGAGCTATCTTACTTACAAAAACCGTGCTGATCTGTCCCAACAACAGCTGCAACACAAGTATGCTAACTTACTGGAGTTGAAGGAAAAGCTGCAAACCGCTAAGCGTGCCTTGGACAAAAAACACCGTGCCATTTATGGCAAAATGGCACAGTTTGTCAGTGAGCTCCGCCAAGAAAAGAAGCAGCTTTTAAGTGCGCAAAAACAAGTTGATGACAAGTCACGTTTACTGGAACAAAACCAACGCCATTTACAAAACCTTTCCAGTGAAACCAAAAAGAAACGCCAGTCTTTAGAGCATGACATTAATAAGTTTGATCAAAGACGCAAGGAAGCGGTTTCTTCCATTTTAAACTCGCACAAAAAACTCAAGCAAAAAGAGGGAGAATTGCAAGGCATTTTGCAAAAGCTTAGCTTGAAAAAAACACAAATTGAACAAGAGTTTTCCAAGCTTTATCAGCAACGCGAAAAGTTAGATCGCCAACGCACTACGCTCTCTAAACTCCACAGAGAGCTAAAAGCCCAAAATGAAGCTACGGCACACAAAAATCGTGAAGTTTTAGAGATTGAGAACTATTACAAGAAGGAGTTGCAACGACTCACCACGGAAAAGAGTGAGTTTGATAACAACAAAAATCGCTTGTTTGAATACTTCCGTAAAATTCGCAATGAAATTGAAAAGAAGGAAGCACACATTAAAACCGTTTTAGAAGAAACACAAAAAAAGCGTCACCTAGTCGAAACGGAAGCGGTCAAACTACATTTACAAAAGCAATCTATCATTTCTAAGGGTCAAGAACTTAAAGAAATCAAGGAACGGGTTAGCCGTGACATTAGCCACACCAACAAACAACGCGAAGAGTTAAACAGCTTATTGCACCAAAACAAACTGTTGCAAAAGAATTTGGCTGAGCGTGAACGTGAAATTAATAATAAGGATTCACTGTTAACCCAAAAGATTCAAACAGCTAAACAAAAGTTAAGCGAAAAAGAAGCACGCATCTTAAAGCTGTTGGAAAAAATGCGTGCGGTAGAACAGCAATACCAAGCGGAAATTACCCGTTTAAAGACACGTAATGCTGATTTGGAGAAGAATGACAATAAGCACTTATTTCCACCGTTATTTAAAATTAACGGTAATGATATGAACTATCCTTACCCATATCCTTGATTTTATCCGCAACAAAAACAAGAAGATAGTTCAAACCAAATTCGCCATCTTTTTGAACAACAGTTGCAATTTATGCAACAGCGTTACGAAAACGAATTAACTGAGTTGCGTCGCCAACGTGCTTTATTGGAAAAAAAACTAGACCAAATTCAACTCGAATCACAGTTAAGCGCGAAGAAGAACGACTTTGAAAAGGTTGAGCAAATGATGCAAAAGTTGTTGGAAAAAACTGAGCAAAAACTCAGTGCCTTTGACCAAAAAATTAATGCTTTGGCAGAACAGATTAACACACAAAAAGCAGAGCACGCTGACAGTGAAAAGCAACAGTTGTTACTAAGGATTGAACAGTTAGAAAAGCAAAACCTAGCTCAAGCTGTTCAAACACCCCAACCAGTACAACCAGTGGTACAAGCACCAGCTGTAGTACCCCAAGTGATCCAACCACAGGTAGTGCAGTCCCAACCAGCTTTTTTAGCAACGCAGCAAAGTATTTCTAAACAGCAACAAATCGCGCAATTAAACGCTGAGATCAATAGTATTAAAAAGCTAATTGCCCAAAAAGCAGCTAAATAAGGAAGAGATGAAAGATGACTAATGATTACCAACAATTAAATTATTTAGTGGAAACTGATGATGAAGCTGACATCATCATAGCTAACCTCGTCAAACAACTCAATGAATTGAAGGAAATTCTGCTTTCATTGGACAATCAAGATTTAGAAATTAATCAGGTTAACCATCGCACCACAGTGCACAATACTAGTTCTAACACTTCTAATAATAGCACTAGCAATAATATTGCTGATGGTAATTACAACAGCAATTTCTTTCACAACTTTAGTAAAGAAACGCTGCAAACACAAGCAAAACGTGGCTTTCTTTTACTAGAACGCTGTTCCTTGGTAGGTCTACAACAATTGGAGTTGGAATACCTCAACGTATTGGGTCGTAGCTTTGAATCACACCAAGACAAAATTAACTTTTTGGTCAATTTACGTGATTTAACTAACGATCACTTGTTGGACACGGAAAAGATTGTCAGTACCCTAGAGCAGATCTTTAACGTTATTGGTGGCACGGAATACACCCCGATTCTCAATTCCTTTTTTAACCAATCACTTAACGATCCTGACCCAATTCAGCGCGAAATTGGACTCAAACAGTTTGTGGCTAACTTACGCCAACGCTTTAAAACGCTATTGCAAAAAACTAACAACTCAATTCAACAGTTAGAAGCGGAAATTCAAATTCCAACTACTCATATTAAGAGTGATGAGGTAATGTTTGGCCCACCGGACATGAACGAACGCTTAGTGTTAAATGACAGTGAAACCGATGCCATTCTACGCTCAATTGAAGCGGAGTTGGAAAGCGCTTTACAAAATAAAAAGCAGGTAGTGGTTACAGCAGTCCCACCAATAGCTGCTAACTTAGCTACCGATAGTATTAGCCAAGAAACTTTAGAACACAACCTTAATAACACTGAAACGGTTAACACCACTACTGTCACGGTAACATCAGCTAGTGAAACTCAAGTGCGTAAACCACAGATTTCATTACGCCCCATTTTTCAAGGCAACTTTCCCAAACGCTTAAGTAGAGAAGACATCCAGCGCTATGCCCACCAGTTACAGGAATTAGAACAAAGTAATGAAGGATAGTGCACTAACACTAAAACGGGTACGAATTGGAAAATTTTCCGAGTCAATGGTTGAAGAACGACCAACACTCAACCTGTTCGAAAAGGTCGAATTCAACCCAGTACCGACTGCTTTAGTGGATCAATTGCCAACTGAACCACTTGTTGAAGCAACTCTTTTAGAAAAGGAAGCAATTACTTTTGTCGATACTTACGCAACTTCGGAAGCTCATGATCAAATAGCTACCTTTGTTTTAGAACAATCAATGGAAACGGAGGTGGTTGAAAAAGAAGCGATTGAAACAGCAATAGTTGCACCAGCACCAGACTTAGTTGAAGAGAAAGCTGTTTTAGTGGAAGAGGTTTTAGTTGAACCAACTGCAACGGAAGCAGTTACAACTGAAGAAAATCAAGTTTCAACTACATCCGTAACTAAGATTAAAACTAAGCGCAGCAATACTAAAAAGGTGACATCAGAAACACTTGTGGCTTCTAAAAGTGTGAAAACTAAAAAGTTAATTACCCCTAACCGGGTAAGTTCGGGCAACGTTAATATAACACTTTGACAAGTAGACAAGAAGTCCACTAACCTAACTAAAACTAAAACCGATCTCTTTGGTAAAAAGCACCAGTTTAAGGGTCCACAGTTAATTTCTTACAAGAAGTAAGCAAAACAAAAGCCAGGCACTAACCTGGCTGTTTGTTTCGGAGAATCTAAACAAGTATTTAAATTTAAGTATTAAGTATTAATTAAACGGGAAAGGAATAGGACGATTTACCAGGGGTTAAACCTAATCGGAACCGACCGGTACTAAAGTAGTCTTTTTGTGAATTCCTTCTTTTATAGCTATAACTGCTGCCATTGCCGTTGGCATTATTAGCGTTAGCAGCTTCGCTGATGTCTAACGCGGTATTAATTAACGTACTAAAGGCGTTTACAATCATAATTACTCCCGCACTGAAGCTTCAAAATGATAAGCCTCCAACTACTTGTTTAGCTTCTGATTTTTTTAATCTCTGCATTATTGTGTCTCCATTTAAATGAATTACATAACTAAAGTAAAATGCCAAAGTTTTATAATTAACTTAACTGTCATCATAGCTCAATAGGACAGAGTATCAGCTTGCGGAGCTGAGGGTTACAGGTTCGATTCCTGTTGGTGACGCCATAATACTTTCTAACCTACCAGTGTTACCCTGGTAGGTTTTTTATTTGCTCCGTTGGCTCACGGTGGATGAAACCACCTTTGACTTACTTTTGTAAACCCAAAAAGTGGTCTTGTAAACCACTTTGGTGCCTTTTACTTTGCTGTTCCAAAGCGCTTTTTTTAGGTTTGCTGGCAGTTCTTTTAGCCAAAAAAGTTTTTTTATTCTAAGATATAAAGTGTTTTAAAGTGTCACAAAGTGGCATAAAGTGGTAAAAATGCTTTTAGGAACCTTCAACATTACCCTTGATGCAAAAAACAGGATCAGTCTGCCAGCGAAGCTGCGGGCTTTCTTTGAAGGTAGCATTGTTATCAACCGTGGCTTTGAAAACTGCTTGGAAGTGCGTAAACCACAAGACTTCCAAAAGTACTTTGAGCAGTTCAACAGCTTTCCCTCCACCCAAAAAGACACTAGAACACTCAAGCGCTTAATTTTTGCCAACGCTAACTTTGTGGATGTAGATACAGCGGGACGGGTTTTAATTCCCAACAATCTGATTAACGATGCCAAGCTAGACAAGGAAATTGTTTTAATTGGCCAGTTTGACCACTTAGAGATTTGGGACAAGAAGCTGTATGAGGACTACCTAGCCAACTCTGAGTCGTTGGAAACGGTAGCGGAAAGGATGAAAGATGTTAAGTAATCAACCCCACAAAAGTGTCTTACTTGATGAAGTCATCCACAACCTCAACATTAAAGGTGATGGTAACTATTTAGATCTTACTGTTGGTTTTGGCGGTCATAGTCAGCACATTTTAGAGCAGCTTACCACTGGCACTTTAACTGGCAATGACATGGATAAGGACAGCATTACCTTTTGTACCGAGCTGTTCAAAGACAAAAAGAACGTTGTTTTAGTCCATGATAACTTCGCTAACTTTTTTAACCACTTAAAGCAGCTCAAAGTAACGAAGTTTGACGGCATCTTAATTGATTTAGGGGTTTCGAGCTACCAGCTAGATAAACCAGAACGCGGTTTTAGTTTTAAACACGCCGGTCCATTTGACATGCGCATGCACCAAAGCGATCGCGTACCAACTGCTTTAGATATCTTGGAGAGGTTGTCTGAGGAAGAATTGACACATGTTTTAAAGAAGTTTGGTGAAATAGCCCACCCCAAACCAATCGCCAAGGCCTTAAAAACACTCATTAATAACACTAAAAATCCAACTACGGTAGAGGTAGCGGAAACAGTTAAGGCAGCTGCTAGCAACTTTGAAAAGTACAAATCACGCAATTACTTAGCTAAAGTTTTCCAAGCCATTCGCATTTACCTCAACCGTGAACTGGAAAGTTTGGAAATTGTGCTGCAACACATTCCCAAGCTGTTGAACAATAAGGGTCGTTTTCTAGTGATTGTCTTCCATTCACTCGAGGAAAAATTGGTGCGGAACTACATTTTTAAACTTACTCACTTTGTCCAACCCCCTGAACTACCCGTTAAATTAACCCCACCGTTTGAACTCATTACTAAAAAACCCATTTTGCCAACTGAACAAGAAATTAAAACGAACCCCCGTGTTCGAAGTGCCAAACTGTTTGTCATTGAAAAGAAATAACTATGTATAACCTCAAAAACATCTACGCCTCCATTACCCTTTACCCCCATGAAATTAACTTGGTGGTATCGGACAACAACAATCAGTTTCATGTCTTGTATGAAAACTCGATTGCTAACGATGAGCTTTACACGCACGCGGGCATTACTAATAAGGCAAAGTTCAAGGTAGTTTTAAACCAGCTCATTAACAATGCAAACGATTATTTGGGTTTTAAACTCGAAAAAGTAATTGTGGTTTTAGCGGAGTTAGTTGATGATCTTGAAATTAACCGCTTTAAAACAGATGTCTTTTTCACTGGTTATGACTTTAACCACCAGGACATGTTGAGCAGTGAAAAGAACCGCTTCTTGCTCAAAAACCGTCATATCAACCCCAATGAAGTTTTGGACTTAGTGGCTTTAAACTACCGTGATTTAACCACTGATAAAATTAGCAAAAACTTTAAGTACAACTGCAGTTATCGGGCAAATGTAATTTATTACACCACTAAAAACTCACTAGTCAAGGAGCTCAAACCGTTTTTAAAGCGCAATATTATTGTCAAAATTGACAAAATTGTCACCCACCACATGGTGTTAGCCCACTCAATTAAACAAATCAACAAAAACAACCTCTTTGTGTATTTAGGGGAACACACTACTGACCTCATGTTGTTTATGGACAATGCCTTAGTTGATTTAGTAAGTGAACCCTTTGGTCGGGTTAACTTCTTGGAATCTGACAATGACAGTGAAAACAAAGCGTTGTTGGAATTCCTAGTGGACTCTACTGCACGCATTGGTGACTCGGGCTCGATTGGCATGGCTTACACCGATGGTTCTACTTATAAGGAAATTAGAGCTGTTACGATTGATGACCTCATTCAAAGTGTTAATGAAAAAACGAAGTACTTAATTGATTTCATTAACACTAACACGGAAATCTTCTTCCAAAAGTACGGCTTTTTACCCAGCACTTTACTGTTTTACACTAAGAGTAAACAGTTGCTTAACAACTTTCAACTCAACCAAAACCTGTTGAGTAACCACTTCAAAACTGTAACCATCTTTAAAAACGAAGTTCAGTTTGTGAGTCGCAAATACTTATTGAACTGTCAAGCAATTGCGCTTTCTTGAAGACAAGACCAAATTAATGTTAATCCCACTAGCTTTGCCCTACCGTTTTACTCAGACAAAATTGAAAAGCAGTTTAAAAAGTCGTTTTTAATGTTGAAGGTACACACGCACATTAATAAGTTAGTGCAAAAACTAATAAAGTAAGCTTTATGGATTGAATACAAACAGCAGGTGCTGGTACTCAACTACCAGAGAACAACATTAAGATAGCCGTTTTTGGAATTGGTGGTGCTGGTAACAACATTATTGATGACATGTTGCGCATGCACCCCGAATTGCAAACGGCTAACGTCCAGTTCTTTGCTTTAAACACTGATTTACAGCACTTAAAAACAAAGCGCTATGTCCAAAATAAGGCTGTGATTCAGTTTGAAGAATCCAAGGGCTTAGGTGTTGGTGGTGATCCCCAAAAAGGTGCCGTTTTAGCACACCATTTTTTAGAGCAATTTCACAAACTTAGTGACAGCTTTGATTTCTGTATTTTAGTCGCTGGTTTTGGTAAGGGGACTGGTACTGGTGCTACCCCGGTTTTTAGTAAATTTCTTAGCAACAAAGGGGTGTTAAATCTCTCGATTGTCAGTTACCCCGCAATGTGTGAAGGGTTAAAGGCACGCGAAAAAGCAGCTAAAGGTTTAGAACGCTTAAACCAAGCGACTGACAGTTTTATGCTGTTCCGCAATGATCGCTGTACTGATGGCATTTACCAACTAGCAAACGTTGCCATTGTCAAAACAATTAAAAACATTATTGAACTAATTAATCTACCACTACAGCAAAACATTGATTTTGAAGACATTCGTTCTTTCTTTAAAAAACCAGCACAACGTTTAGAGAATGAAGCTAACTTGTTTCGCGTAACTAATACTTTTACTTTTAGCTTTGACGCGCACAATACAATTGAGCACTTTAGCCACAAATTGAAAAACTTTGAGTATGAAGGTTTTTTTGACCATAAGGTAGAAGGGGCGCAAAAGGTAATTTTAAAGGTGTTAGTCAACCAAGGTTTATATCCGCTTGATTTAACCCAAATTCAAGAGATTATTTGAGCCAAAATTGACAATCATAACCTAGAGGTACAACTTGGAGTTGACTTTACTGATGCCAACCCTTCAGTGCAACTGTTTTTCTTAATGGAGAAAAAGCAAGCAGTGTCCTCTGACTTTATTCAAAAACCAGCATTTATCTCAGTTAAAGAAGTAAATCAGAAACCGGCCAAACCGTTCCAAGTTTTGAATGATTTAAAGGAGCTGGGACTGAAATACGTCAAACAGCAAACCGGATTTAATTATTAGTGGTCTTTCTTCTTTAGAATAAGCTAACTAATAAGAAGGTTAATGGCTGTTGCAAGTAAGAAGTTCACTGAACGTCAATTTTTATTTTTTGTTGTCAACTATATTGCTGGCTTTGGTTTTATTTCCACTACCCTATCACTGTTTGAGCTAGGACCACTTTCATGAGTGATCTTTTTTCTCGTTAGCTTAATTAGCCTCATTGTTACCTTAGCATTTGCCAGGGTATCAGCGATTGACAAAAATAATTACGGCGGCGCTTACCTGTGAGCCAAAAAAGCCTTTAACAGTGAACGGTATTCCCACCGCTTGTTTATCTTCTTTACCGGGTGAAACAATTTTATTGCTTCACCGCTATACGCCGCTATTGCCCCGCTTTTTATTCTCTCTGCTTTTAAAGGTATTGATGGTATTCAGGGCAATGCAGCTAACACTTGAATCCTTATTGCCGCTGGTTTTACCTTTTATTTACTGTTAGCCTTTCTTTCCACAAAGGGAACTTCCCTCAATAAAAAGTTAATTGCGGTATTTGCCTCAATTAAATGGGCGGTGCTTTTGAGCACCTTAATAGTAGCACTTATAGTTATCGGCAAAAATGGTAATGGTTATGCCATTAACAACGACATAAAACAAGGCCCTTTTAAAGAACGTGAAATTACCTTGGGTGCAGTTGCTACTGTCTTTATTACCTTCTTTTATTCCTACAGTGGTGCGGAGGACATTTCAACAATGACACCGGATGTGAAAACCACTAACTTCCGCAAGATTTTAATCATCTCTTTTATTGCGGTATTCTTGTTTTACTTTGTGGGCATTTTGATCTTTAATGGTTTATTGAATGTTTCACAAAGCGATGATGATAAGGGGAAAGATGGAAGAATAGCTAGTGTAGCTGATATTTTTAGATTAGCTGGCGGTTTAGGGGCACTCATCTTTTACGGTGTGGGTGCTTTATTCAATAACGTATCTACCAGACTTTCGACCATTATTGCTAACTCACGCAAGATCTTACCGTTAGCACAAGATAACTATTTACCCACTACCTTTAGCAAAGCTAACAGTAAGGGCGAGTTCCAAAATGCAATTTGGTTTACCTTTGGTACTACCATCATTACGATGACGGTACTGTTCTTTGTTCCGCTAATAACCAGCAACTTTGACTTCGACAAATCAACCGAGTATGCCGCCTCGATAGGTTCGGCCGCTACACTGGTACAATATATCCTTGTATTTTTTATAATTTTTAGGCTTATTTATAAAAAAGAGCAACTTTATCAAAAAAACTGGACACGCGAGTTAGAACAAATACTGTTTGGGATTGGTGTCTTTATTATTCTTCTCATGTTGCTCTTTTATCTCTTTCCAGTAATTGATGGAAAAACTAAATGGCAATTGAAAAATACCCTCACTTTAGTGGTGTATGGGGTGTTGATTTTAATTGGATTTGCCCTCTTTATGACCCAAGAATATAAGCGCAATAAACAAAAGGAAAGCTATGTCACACCAAGAGCAGTTACATAAACCTAACCGGCAACAGTTTAGCGAAAAGCAATTTATTGCTTTTGCTTTTAACTATGTTGCTGGGTTCGGCTTTATTTCCGTGGTGCTAACCATGTTTAAGCTTGGCCCGTTTTCTTACTTGATCCTAGGGCTAGCAGCTTTAGGTATTTTGGGGGTAATGTTGTCCTTTTCGCGCCTTTCAATTATTTGTGGTAGCAAGGCTTACGGTGGTAGTTATTTAATAGCCAAAAAAGCACTTGGTGTGAAAACAATCACGGCGCGTTTCTTTACTTTTTTGAGTGGTTGAAATGTTTCCTTAACCGGTCCTTTTAATGGGTTAATTGTGCCAGCGGTGCTAGTTCTCTCGTTTGCTGATATTAAAGCAGTGAAGGATAACAATGGTGCTTTAATTGGCTTACTGGTCGGGGGCTTTGTTTTATTTGGGGCATTAAACTTCATCTCCCTGTTTGGCCTTAAAATGAATAAGAACGCCATTCTCTTCTTTGCCATAGTGAAGTGAGTAGTTGTTCTGGGCGGTTTAATTTTGGGAATCTACCTCATCGGTACGAACCACGGCCATGGTTTTGTCGAAAACAACACTTTAGGTGAACACATTGAAGATCTCAGTTTCCTTAAAGTTATTTCTACTACTGTGGGGATGTTGGTGGCCTTTGCTGGTACCGAAGATCTTACTGCGATTACCCCGGATGTGAAGTCAAAAAACATCCGTAAGTGTTTCTTATTGATGTTTGGTGCAGTTACCCTGCTTTATCTAATCGGTTTTGTCATTATTAGTGGGATTTCTGGTTTAAATGGTTATGGCCTTGATGGTAAAGAAAAGAATGAAAAAGCGATTAACACCTTTGGTTCGATTTACTTTCAAGCAGGCGGTAAGTATTTAGGCATCCCACTGTTGGTCATCTTTGGTCTGGGTTTTTTACTCAATTCCTTGGCATCACGATTAGGCATGACCATTACGACAGCGCGCAAATACGTTGCGTTAGCCCAAGATGGTTTCTTGCCGTCCTTTATTAACGAACAAAACAAGCACCACGAGTACCATAAGGCAGTGTGAGCTAGCAACATTATGACCTTAGCAGTGATGGTGCTGATGATCATTGTCCCCTTTTTACCTAATGATGAAAACCCCGGTAAACAGCTCGTTATGTTCGATGCCATTAGTGTTTTAGTTGAAGTGGCGATTGAACTAGCAGTGTTAATTTCCTTAATCCAGTACTTTATTACCTACATCTTCTTCTTTATGATTCTGGCCAAAAAAGAGGGTAGTGCCAGTGTTTCGTGATGGGAAATTGCCAGTTATGGCGTAAGCTTTGCCATTATTACGGTTTTGTTGTTTGTTAACCTCTTTCCTATAACGGCTTGAAAGAACACCAACACCTTTAAGCTAAGTATCCTAGCGGCCTTCTTTGCCTTGGGTATTGGCTTCTTTATCCACTCGGAAATAAAACACAAAGGACAATTAGTCAAGTCAGTCGAGTGTAATTAAGATCACTCCATTAAACCGGTAGAAAAAGCCGTCACGCATTTCGGGTGGCGGTTTTTTCTTGTTCTTTTGACTGCCATAAGACAAAAAATACAAAAAGCATTCAGCGTTTATCTCTTAACCACCGGTTTGAATAGTTAATTGAGCTGCTAAAACGGCTTTTTAGTGTCTTATAAGCGCTTAGAAAGCCTTTTCTGTGCCATTTTCCTAGCTTGAGCCTAAAATGAGCCCTACAGGCCGTTTTGGGGGCTTACAAGCGGTTATTAACATTGTTTTAACAGGTTATTAACATTGTTTTAAAAGGCTCCATTAGCTCATTTTTGCAGTGTTAAAACCGCTTTAAAGCTTATAAAGCTTTAAAAAAAGGCGTTTAAATGAGCTTTAAAATAACCGTTTTAAGTTATTGCAAAAACTCTTTTAAAAAAGTTATTCGCTTATTTTGCAAGCTTAAAAAAAGATTTTTATACAATGCTACACCATCGAAGTTTAGGAGAGTTATGCAGCAGTATCTAGATTTAGCCCGTTATGTTTTAGAGCACGGAAAATACCGTCCAAATCGCACTGATACTGCTGGCATTGGTGTCTTTGGTTACCAGATGCACTTTGATATTTCAAAACACTTTCCGCTCTTGACTACGAAGAAGGTGCACTGAAAGTCGATTGTGCACGAACTTTTGTGGTTCATTAAGGGTGACACCAATATTAAGTACCTAGTCGACAATAAGGTCAACATTTGGAATGAATGACCTTACGAAAGCTTTAAAAAGTCACCTCACTTTAACGGTGAGAGTCAAAAGGAGTTCATTGAACGGATTCGACAAGACGCGAAGTTTGCCCAACAGTTTGGTAACCTCGGACCCGTTTATGGAAAGCAATGGCGTGACTTTAACGGAGTCGATCAATTGAAAAAGGTAATTGCGCAAATTAAGGTAAATCCGTTTTCGCGGCGCTTAATTGTGTCTAGTTGAAATCCCAATGAGGTGGATCAAATGTTGCTCCCGCCTTGTCATTCACTGTACCAGTTTTACGTACAGGACGGGCAGTTGAGCTGCCAGTTGTACCAGCGCAGTGGTGATATTTTTCTAGGTATCCCCTTCAACATTGCGTCCTACTCTCTTCTGGTGTACTTAGTTGCTAAGGAAACTAACCTAAAACCAGGGAGCTTTGTCCACACCATTGGCGATGCCCACATTTACGAAAATCACTTGGAACAAATTAAGTTGCAATTAACACGTCAACCCAAACCATTGCCCAAGGTAGTTTTAAAGAGTGATAAATCAATTTTTGATTATCAATTCGATGATATTGAACTGGTGGATTACGACCACCACCCTACAATTAAAGGAGAGGTAGCAGTTTAAGATGGTAAAAGCAATTTGAGCAATGGATCAAAATGGGTTAATTGGTAACGGTAACAGTTTGCCCTGAAGGATTAAAGCGGAGTTACAGCACTTTCGCCAGACCACGTTGCACCAAGATGTATTAATGGGCTCGGCTACCTATCTTTCCTTACCGCCGGTGTTTAGTGAGCGTAACGTTTATATCCTCACCCGTAACCTCAACTTTAACCCGCCTGATAAGGGCTGTTTGACTAAAGTAATCCATGAATATGAAAACTTTATCCAACCTTATTTGCACCATCCCGATAAACACCTCTACATCTGTGGTGGGGCACAAGTTTATGAACAGCTCATTCCCCGGTGTGATGCGTTAATTGTCTCTACCATTTTCGGTAAATACACCGGGGACAAATACCTCAAAGTGGATTTTAGTCCCTTTGAATTGACCAAAGAGATCAGTTTTGCTGAGTTTAAGGTCGCTTACTATCACAAAATTGCACGATAAAACAATATGGCTAACACAAAAAAATACTTTTTAGAATCAGTTTCTCCCTTAGAATATGCACAAAAAAAGTTTCAGGGCAACTTGCGCTCGGTTAACTGAAACCTAGTGGATGATGAGAAGGATCTGGAAGTGTGAAACCGGATCACGCAAAACTTCTGGTTACCAGAAAAGATTCCGGTATCCAATGACATTCCATCATGAAAGCAGCTGTCTAAGGAATGGCAGGATTTAATTACCAAAACCTTTACGGGGTTAACATTACTTGACACCATCCAAGCTACCATTGGTGACATTAAGCAAATTGACTATGCTTTAACAGATCACGAGCAGGTAATTTACGCCAACTTTGCCTTTATGGTGGGGGTACACGCGCGCTCTTATGGCACCATCTTTTCCACTCTGTGTACTTCAGAACAAATTACTGAAGCACATGAATGGGTGGTGAAAACCGAAAGCTTGCAAAAGCGCGCTAAGGCTTTAATTCCCTATTACACCGGGAAAGACCCATTAAAGTCTAAGGTAGCAGCCGCTTTAATGCCCGGGTTCTTACTGTATGGTGGGTTTTATTTACCGTTTTACCTCTCCTCGCGTAAACAGCTACCCAACACTTCGGACATTATTCGCTTAATTTTGCGCGATAAGGTAATCCATAACTATTACAGTGGGTATAAGTTCCAGCGTAAAGTGGAAAAGATGTCCAAGGAAAAACAGGCTGAGATGAAACGGTTTGTCTTTGATTTAATGTACGAACTGATTGAGTTGGAAAAGGCATACCTCAAGGAACTGTATGAAGGGTTTGGGATTGTAGAAGATGCCATTAAATTCAGTATTTATAACGCTGGTAAGTTTTTGCAAAACTTAGGGTATGACTCACCGTTTACAGAAGAAGAAACGCGGATTAAACCAGAAATTTTTGCCCAATTGTCAGCCAGAGCCGATGAAAACCACGATTTCTTTTCTGGTAATGGTTCGTCATATGTGATGGGAATTAGTGAAGAGACCGAAGATAAGGATTGAGACTTTTAACAATGCATAAAGATATCAAAATAGTCGACGCTAGTGCGATCGTTAAACCAACTGGAACACCGTATGTAGTGTATTTTTCCTCCATTTCTAATAACACCCACCGTTTTATTGAAAAATTGGAGTTTGAACACACCCGCATTCCGGTTAATTTAGATGAACAGATTGAAGTAAACCAGGAGTATGTGCTCTTTTGCCCCACTTACAGTGGTGGTGGGGAATACACATCGGGGGCGGTACCCAAACAGGTAATCCACTTTTTAAACAACAAGCACAACCGTGATTTGTGTCGTGGCGTAATTTCGTCCGGGAACACTAATTTCGGCAATACTTTTGCCATTGCCGGGCCGATTTTGTCGAAAAAGTTAAACGTACCACTTTTATACCAGTTTGAACTTTTAGGCACGAAAAACGATGTAGAACAAGTTCAAACCATTATTACCAATTTCTTTGGTAAAGCCAAATAACCGTTATTAATGATTAGCCAATTATGTCAGTTAAAGAAAAGATTCCTGCTTTCAACACCCAAGAAGACTTAGAAAGCTATATTTCCTTAAATGCCTACACTAAGGTGTACGGTGATTTTAAGATGGATTTACACGCTGTGGAGGCTTACATCCAAGAGCATGTCAAACCCAAAACAAAGGTGTTTCACTCCACCAAGGAACGCTTAGACTTTCTAGTAAAGAATGATTATTACGATGAGAACATCATCAACATGTACAGCTTTGAGCAGTTTGAGGAAATTACACGTAAGGCTTACGCTTACCGTTTTCGTTATGCTAACTTTATGGGGGCATTTAAGTTTTACAACGCTTATGCACTGAAAACCTTTGATGGGAAGTGGTATTTAGAAAACTATGAAGACCGCGTTGTCATGAACGTGCTCTTTTTAGCCAATGGTAACTATAACAAGGCTTTAAAGTTACTCAAACAAATTATTACCAACCGCTTTCAACCAGCTACCCCAACTTTTTTAAATGCGGGACGAAAAAAACGTGGTGAGTTTGTGTCGTGTTACTTACTGCGCATAGAAGACAACATGGAATCAATTGGTCGTGCCATTACCACTACCCTACAGCTCTCCAAGCGTGATGGTGGGGTGGCGTTATTGCTCACTAACATCCGCGAATCGGGTGCGCCAATTAAAAAAATAGAAAACCAGTCATCAGGCATTATTCCAATTATGAAGCTGTTGGAAGACTCTTTTTCTTACGCCAACCAATTAGGGCAAAGACAGGGAGCAGGAGCAGTGTATCTCCATGCCCACCACCCCGATGTCATGCAGTTTTTAGACACAAAGCGCGAAAACGCTGATGAAAAGATCCGGATTAAGTCGCTGTCTTTGGGTCTAGTTATTCCCGACATTACCTTTACTTTAGCTAAAAACAATGAGGAGATGGCCTTGTTTAGTCCGTATGATGTGTATGAGGAGTATGGCAAACCCTTGTCAGACATTTCAGTCACGGAAATGTATTACGAACTGTTAGCGAACCAACGCATTAAAAAGACCTTTATTAATGCGCGCAAGTTCTTCCAAACAGTGGCGGAACTGCACTTTGAAAGCGGTTATCCTTACATCTTATTTGACGATACGGTTAACCGGCGCAACGCGCACCCCAACCGCATTGTCATGTCTAACCTCTGTAGTGAAATTGTCCAACCCTCCACTCCATCGGAGTTCCACCACGATTTGGCTTTTAAAAAGGTTGGTAATGACATTAGCTGTAACTTGGGTAGCTTAAACATTGCCAAAGCAATGGAAAGCGGACCGGAGTTCTCGGAGTTGGTTAAACTAGCGATCGAATCACTTGATTTAGTTTCAAGGGTAAGCAATTTAGAAACGGCTCCATCGATCCAAAAGGGTAATTCCGAAAATCATGCTTTGGGATTAGGGGCAATGAACTTGCACGGTTTTTTAGCAACTAACCAAATTTATTACAACTCCCCTGAAGCAATCGACTTTACTAACATCTTTTTCTATACGGTAGCTTACCATGCCTTTAAAGCATCCAGTGAGCTAGCTTTAGAGAAGGGTAAGTTTAAAAACTTTGAAAACACCAAGTTTGCTGATGGGAGCTACTTTGATAAATATATCAAAGTAGAACCAGACTTTTGAACACCCAAAACCGAGCGCGTTAAGGCGTTGTTTCAAAAGTACCAAGTGGAAATACCAACACGTGAAAACTGAAAGGAGTTAGCACTCAACATTCAGAAAAACGGTTTGGCCAATTCCCACTTACTGGCAATTGCTCCAACGGGTTCAATTTCCTATCTCTCCTCCTGTACTCCTTCACTCCAACCGGTAGTATCCCCGGTAGAAGTGCGCAAGGAAGGTCGGTTGGGACGGATTTATGTACCGGCTTACCAACTCAATAAAGACAGTTACCCTTTTTATAAAGATGGTGCTTACGAACTGGGTCCCGAACCAATTATTAACATAGCAGCAGCGGCACAACAACACGTCGATCAAGCGATTTCGCTCACCCTATTTATGACTGATAAGGCTACCACTCGTGACCTCAATAAAGCTTATATTTATGCTTTTAAAAAGGGTTGTTCTTCCATTTACTATGTCCGAGTGCGCCAGGAAGTACTAGAGGACAGTGAGGATCACACCATTCAAATGCAGCAGTGTGAGGCCTGTGTGATTTAATAATACATATAAATACATATAGTAAGTCTAGCCTTAAGCATGACTTTAGCAGGGGTTAATTTGTTATAATTTCACGCTTATTTGCGTCTTCTGCGGTTCCTAAATTTTGTTTATACAAAATACCAAAAGAAACGGTTTTATTGAAAATGCATGCTATTGTAGTCTGTGGATCAAAGCAATATTTAGTCCACGAAAATGACACTTTTTTTGTTGAAAAACTTGAAGCGCCAGTTGGTAAAGAAATCCAACTCGACAAGGTTTTAATGTTGGATGAAAAGATTGGTGCGCCATACTTGGAAAAAGCGCGAGTAGTGTGTGTGGTGGAAAAGCACGGTTTACAACGCAAAGTAAACGTGATTAAACACATCTCACAAAAACACCACTTAAAAAAGTATGGTCACCGTCAACCTTACACTAAGTTAAAGGTAGTGCGCTTTGTCCATGATTAAACTAACTGTGTCGCACCACAAACTCACCGCTTCCGGGCACGCTTTATTTGCCAAAAAGGGGCAAGACATTGTGTGCGCTGCTGTTAGTGGTATTATCTTTGGCGCTTTGCCCTGGTTTGAAACCAACAGCATTGCCGTTCAAGAAGATGCTACTGTACCCAGTTTAAGCTTGGAGTTAGTGCAACCAACCGCTAAGCTTATCACGGGATTGAGTGTGGTGATTATGCAACTTAAAACCCTCGCGCATTCTTACCCGCAGTTTATTTCGTTTGAAGATCAACGTAAAGATGAATAACAAATATTTTTTAACCAAAATTGACTTACAGTTTTTTGCTTCCAAAAAGGGAGTTGGTTCAACCAAGAACGGCCGTGATTCTCATGCTAAGCGTTTAGGTGCTAAGAAGGCTGATGGTCAGATGATCCGTACTGGTCAAATTATTTACCGCCAACGCGGTACTAGGGTATATCCCGGTGTAAATGTTGGCCTTGGTAGTGATGACACCTTGTTTGCTTTAAGCGACGGCTTGGTAAAATACCAAAAGTTTGGACCTAAACAGGGCAAGACCCGCGTGAGTGTGGTCAAACACAAACTAGATGCCTAAACTCCTAGGAAGCTTTATTTCGTTTAAATCACCCCATTATCTAGTGGGGAGTGTTCGTGACGCTGTTAGCATTAAAGCACAAGCGTTCATGATCTTTTTGGGCGCACCACACACGGCACTACGAGTCGATCCTAATCGTATGCAAATTGACGAGGGTCACACATTGATGGAACAACATAACCTCAGTAAGTCTGGGATGGTTGTCCATGCTCCTTACATTATTAACTGTGCCTCCAAAGATCCGGTAAAGCAAACCTTTGCGATTGACGTTCTGACAAGGGAGGTTAAACTGTGTCATGCCGTGGGTGCTAAACTAATCGTTCTCCACCCCGGTTCAGCGGTTGAGCAAACTCAAACCCAAGCGTTAGATCACCTAATCAAGGTCTTAAATACAGTGATTGCCAACACTAAAGAAGTCATTATTTGTCTCGAAACGATGGCTGGTAAAGGTAATGAAATTGGCCGTGATTTAGACCAGTTAAAGTATGTGATTAACCACATTGAACAACAAGAGCGGATTGGTGTTTGTCTAGATACCTGTCACTTCCATGACAGTGGTAATGACTTTAACAATACTGCCGAAATTATGGAAACGATTGACACTAAACTAGGCTTTGAGTTTTTAAAGGTCATCCACTTAAACGAGTCCAAAAACGTTTGTGGTTCCAAAAAAGACCGTCATGCCAATCTAGGTGAGGGGATGATTGGGTTTGATAATTTAATGCGCTTTATTGCCCAACCGCAAATAAAGCAAATACCGATTGTTTTAGAAACCCCATCTGACAAGCATAATTATCCCGCTGTATATGGTGCTGAAATCGAAAGGATCCGTGCTTGATTTGGAGCACGATAACCAAACACTAAAGGATTACATTAGTGTGTTTGAACGCAATAAGATGCGTTTAACCCAATCACGCTTAATGTTGCTCCAATGCTTAGTGCAACACCGTGACTGGCACACCTTAGCTGAGCTCAAACACCACTTGGAACAAAACCAACAGCGCACTACCTTAGCTTCTATCTATAACAACCTCAAGATTTTTGCTAAGTTAAAGCTAATTAATATTTTTGTAGATACCAATCGCTTTGAAACGTATTATTGCTTAAGACACGAAAACCACAAGCACATTTACTTCTTTGACGAAAACCAGCGCAAGTTTTTAACCCTACCACTACAAGACAAGGAAGCGTTGAGTTTAATTGGACATAAAAGTAAACATGGCAAAATTAAACTGAATGACTTTTACATAGTCGCTAGCGGTACACTTGAAGATGATCAATAAGCCAAATCAGTTTGTCAACCACTTAAGTGCTTTAAAAAAACACTTTGCCTCTTATAAGGAGCTCCGTGAAGCGTTTAACGATTATCACAAGCATAACGGTGATGAGTTGACCACCTTCTTTTTGCACCAGTTTGATAAGGTGATGGAACTAGTCAAGCAGAAGGACTTTAAAACTGCCCAGTCTCGCTGTGAGGAGGAATTAGCCGCCCCTTATCTACCAAAACCTTTAGTAAGCTTTTTCCAATCGCTACTCCAGCTCGTGAACCATGATTTATTGGAACAGCAAAACGCGGCCTTGGCTAGTTTACCCGCAGCCAAAATCATAGAGCTTGTTTTACAGGATTACCCCAATAAACTCAACATGATTCACTACCTTTTGCCCAAAACGAAGGCGTTTGTCAAACCACATTTGTTACAACGCTTACAGTTTGTGTTAACTGATTCGGAGTTGTTGGAATTAAAGCGCTTTTCCTTCTTTCAAGCACTCAACCAGATCCCGGGCTTTCAGGGTGAGCAAGTTGAGTACTTTAACAGTAAGCTCAAACAAAAGTTTACCTTGACCTTAGGGGAGTTTGAAATTGCCCAACAGCCAGATGCCAAAGCATACTTTGAGCAGTTAATTACCCAAATCCAACAGCTCTTTTTAAAAGAGCCGGTTAACGCTGAGTTTGCCAATGAGATTATTGATGCCTTTTTGGTGAGCTATTTCCCCCTCCATCCCCCAGTACCTTTGGCACAATTAGCTGCCAAGATTTATGAGTATGTCAGCCAAATTGTGCTTAACGAAGCGGTTAACCTAAAGGATGAACTGATCAAACTAATCGTTCATACGCTTTACGAACAGTTAGATCGTCCTGTTGGGGATGAAAATTAAAATATAGCTCAGATCTAGTTTAGAATGAAACAATACAAGCTTGTCAATACAACCCAAAAAGAAAAGACGCTTTGTCTTGAAATAGCAATTGACACGAAGCTGTGAAAGGAGACACAGCAAAAGCAAACCCAAGATTTAACCAAAAACATGAAAATCAAAGGTTTTCGTAAAGGTAAAGTGCCTCCAACTTTAGCTAAGGATTACTTAGACCGTGCCGAGTTGTTGCAACGTAGCGCCCAAGCGGTAATTGATGCCATCTTCCAGCCGTTACAACAAGAAGCAGTAATTGCTGACAACGAGAATGTAATTGAGGACTTTCCTACCATTGACTTTAAAACAATCAATGAGAACGATTGTGTTATTCTGTTTGACTTTGATCTCGTACCCCAGTTTGAACCACCTGATTACAAGCACATCAAGGATTTAAGCCCGATTGTACCCCTAAAGGATGAGGAGTTCAACAAGGAGTTGCACAACATCGAAAAGAACAAAGGGAAACTAGTTGATGTTAGTGACAAAGCATTGGCCAACAATGATATTGCCGTCATTGACTTTGTTGGCAAGGTAGATGGTAAGGTGCTTGAAAGCGCTACTGCCAAACAATACGAATTAACGATTGGCTCCAACAGCTTTATTGATGGCTTTGAAAGTGGTTTAATTGGCATGAAAGTAGGGGACAAGCGTCAGTTAAAGTTAAAGTTCCCCAAGGACTACCATGCTGAAGAATTGAAGGGTAAACCAGTAGAGTTTGACATAGAGCTCAAAGCAATTAAGCAGTTAGAGATCACCCCCATGGATGAAACTAACTTTAAGGAGTATTTACCCGCACAATACCAGGGTTTCAACTCACTCAAGGAATTTAAAACTTACTTTCATAAGTTAGTTTCCGCTAAGAAACTGGAAATTACCCTACAAGAAAACAGTGTTAAAATTCGCCAGTTCTTTTTAGCTAACACCACCTTACCTTACATTCCTGATTCCTTAATTAAATTGGAATCCGATCGCTTACTGAGAGCGCAAAAGGATCAAGCGGAGCAGTACAAGATTCCCTTTGAGAGATTGTTAGCAGCTTCCAAGCTTTCATTGGAGCAGTTGCAACAACGTAACATAAAAGAAGCACGTGATAACGTTACCTTTGCTTTAGTGATGAAACGAATCGCTGATGTGGAAAAAATCAAGGTTGATAACAAAAAGATTCACAGCGAAATCGAATCGATTATTGATGTTGAATACCCCTTTGTTAATGCCGAGTTGAAAAAACAAATGTTCCACAACATGGAACAACAAAAGGACTTTGTGGAATCGATAATTTTGAACCGTTTAACCACTACCAAGATAATTGAGTACTCCACTCATTAGCACTCAAAGTATTAGAGTGCTAAAAAATGTGTTACAATTTAGCATTCTTCAACCTCTTATCTTAATAACTATGCCAGCTGTAAAAAAACCACAAATTCTCGTTGTGCGTAACCAAGTTATCTTTCCGTACAACGGCTTTGAACTTGATGTCGGTCGTGAACGCTCGAAAAAACTAATTAAAGCACTCAAAAACCTCAAAACCAAACGTTTGGTGTTAGTCACCCAAAAGAATTCAGATCAGCTCAACCCTGAGTTTGATGACATTTACCATTGCGGTACTTTATGTGACATTGATGAAATTATTGAAGTACCGAGTGAAGATGGCAAGACAGCTGACTATAAGATTAAGGGTAAAGGATTACAGCGCGTTGCCATTACAAGCTTCTCTGATGCTGATTTAACCAAGTACGACCATCACTTTCTCAACTCTACCCTAACAGAAAATAAAGCGTTAGATAAGTTGTTGGAACGGATCTTTCCCGATAAAGAAGACTTTGCTGAAATTTTAGACAGCTTAAACAGCTTCTTGGAACTGCAAGAGTTAAAGAAGCTATCTAAGGTACCAAAAGACATAAAACGGTATGACATTATTACCTTCAAGCTTGCTAGCTTAATTTTCAAAGACATTACCCTCCAACAAGCCATTTTGGAAGAAAATGACATTGAAAAACGGTTACAGAAGATCATTGGTTCTGGCATCGAGGATTTAGGTCACATCAGCGAAGAAGCCCGCGCTAAACAACGCGAAAGTGAGTTTGACAAGATTGACAACCGCATCACGCGCAAAGTTAACGAACAGTTATCGCGCCAACAACGTGACTTTTACCTCCGGGAAAAACTCCGGGTTATCCGTGAAGAGATCGGGATGACTTCCAAGAAGGAAGATGAAGTATCCAACATTCGCAAAAAGCTAGAGGAAAACCCATATCCAGAGCACATTAAAAAACGGATTTTAAGTGAGTTAGATCACTTTGAAAACTCCTCCTCTTCATCACAAGAATCAACCTTAACCAAGACTTACATCGACACTTTGATGAACTTGCCATGGTGACAGGAAAGCAAGGACAATGCTGATGTAAAAAACCTGATTAAGATCTTGAACAAGAACCACTCAGGACTAGATAAGGTAAAGGAACGAGTGGTTGAGTATTTAGCAGTACAGCTCCGTACCAAAAAGCTTAAGGGTCCGATTATGTGTTTAGTCGGTCCACCTGGAGTTGGTAAATCCAGTTTAGCCAAATCAATTGCTGAAGCGTTAAACAAGTGCTTTGTTAAGGTTTCATTGGGCGGGGTGCATGATGAATCGGAAATCCGTGGTCACCGTAAAACCTATTTGGGTTCGATGCCAGGACGGATTTTAAAGGGCATGGTACGTGCTAAGGTTATTAACCCACTGTTCTTGTTAGATGAAATTGACAAGATGACATCCTCCAATCAAGGTTACCCTTCCGGCGCTTTGTTAGAGGTATTAGATCCGGAGTTAAACAATAAGTTTAGCGATAACTATGTTGAGGAGGATTATGACCTGTCTAAGGTAATGTTTGTAGCCACCGCTAACTATATCGAAGATATTCCGGAAGCCTTGTTAGACCGGATGGAAGTGATTGAACTTACCTCTTACACCGAACAGGAAAAGCTACAAATTACAAAGAGCCACTTAGTTAAGCGTTGTTTAGATGACGCTGAGATTAAAACAGATGACCTCAAGTTTACCGATGAGGGTATTAGCTATATCATTAAGTTTTACACGAGGGAAGCGGGGGTGAGACAGTTAGAACGCTTAATCCAGCAAATTGTGCGTAAGTACATTGTTAACTTGCAAAAAACCGGCGAACAACAGGTAGTAGTTGATGTAGATTTGGTAAAGAAATACCTCAAGAAAGAAATCTTTGACTATACCGTGCGCGATGAAGATGCACTTCCGGGAATTGTTAACGGCATGGCTTACACCCCAACTGGTGGTGATCTGTTACCAATTGAAGTAACCCATGTAGCTGGTAAGGGTGATTTAATTCTGACCGGCAATTTAAAGCAAACAATGCGTGAAAGTGCTAGCGTAGCGTTAGGTTATGTCAAGGCCAATGCGCAGAGCTTTAACATTAACCCTAACCTCTTTAAAAAGGTTGACATTAACATCCATGTTCCAGGTGGTGGTATTCCCAAGGATGGCCCGAGTGCTGGCGCGGCTTTAGTTACTGCCATTATCTCGTCTTTAACTGGCAAAAAGGTTGACCCTAAGATTGCCATGACCGGGGAGATTACCCTAAGGGGTAAAGTAATGACCATAGGCGGGGTGAAAGAAAAAACCATTTCCGCCTACCGCGGTGGAGTACGCACCATCTTTATGCCCGAAAAGAATGAGCGTTACTTAGATGAAGTGCCTAAAGACATCGTTAAGGACTTGGAGATTATCCTAGTAAAGGAATACAAGGACATCTACAACAAGATATTTAATTAATTTTTACCAATTCAGGACTTCAAGGTGCCTAAGATCAGATAATTACCCTTTTTAAGGGTAAAATGGTCATCATTAAACTAATTCATTTCTTATAAGTGTTAGCTAGAAGAGGACGACGGCAAAAGAGTGCTGTAGGACTTACTTATTTTGCTTACCTTGCACAGCAAACACTCCGCAGCAAAACCTGCTGGGTGATGTTTGTGCTGTTGATGCTGGCGTTAGTGATGCTGTTCATTGTGCAATCGAGCACCAACGTGATTGCGCTTTGAACAAACAATGTCTTTCAGTTTTCACTCATCTTAATACCGTTAGTGACACTGTTAGCGGGCAGTATTACCGCTGACATCTTTATTGAAGGGTACAACAACGGGATTGAGCTGTTCTTGTTCTCTAAACCTATTAGCCGTGACCGCATTTTGCTCATTAAGTTACTAGTGCTGTTAATGTACTTGAGTATTGTAGCCGTACTATCCACTGCTGTAGTGAGCTTGGGTTACTTTACGCGCAATGAGATTGAGAGTTATTTAGATGATTTAGCTTTAAGTATCTTTGTCGGTACCATTTTAAACGGCATTATCTTTAGCGCTGTAACTATTATGTTAGCCACGCGCTTTAGCAACATCCAGAGCTTAATCATTTTGCTCTTGTTTGTGTCGTTGTTTAGCTTTTCCTCACCAATTGCGCGGTTAATCTTCGACACGCCTGCTAAAACGTTGAGCCAACAAGGTTACAGCGTTCGCCAAATTAACGGAATTGCGCACAGCGAAAGCCCTAATGTAGAGCGTAAAATTTACGTTTCGGTCAAAAAGCGGCGTTTAACTGGGGTGGATCGCGAAGAAAAAGAGCTTTGAAAGAGTGATGAGCAAGCAGCACTGCGTGGTAGAAATTTGTATGAGGAGGCACAACGAACCTCCTCTTACATTAATTCCTTTTACACCAACATTGGTTACTTATTTGGCTCGCTTTACCGCTTGGGTGCGTTAGCTGATGAAAATAACTTTGATGCATCAGTAGCAACCCAAAACACCAAAATTCGCTTTGGTAAGTTAGTTGATTTAGATAAGAGTACTGACATTATTGGTTATCGCACTAAAGACCGCTTTGGCCGTTTCTTTCGCTTTCACTTGCGCTTTCCAACGCTCAACTTTAAGCAAAGCTTGAGTAACAGCGGCGTTTTCTTTATCAATAAACGGGGATTAGTTAAAGCTACTTTTAGTGCTGGCGATGATGCCTTTAAACGTGACCCGAAGATAATGCAAAAGGTGTTTGACACTTTTCTAGAAAGTTTTAGTTGATTTTTAGACAAGAAAATTAACCGCCGGATTGCCAGAATTTACGCTGAAAGTGATAACAAGGGTAGTCCTTTCCCAAAGGAAGGAGATGATGAAAATACTAAGGGGGATGACAACAGTAGTGAAAAAACGGATACAGTCTCTGTATCAACTAAGTTAAAAACAACTGCTGATCAATCTGAAAGTACTCAGATGTCTAGTGAAAGCACTGCCACCGGTATTTCTAGTGATCCCCAAAGTCAGGGCAAAATGAACAACAAAAGTGAGGAACAAAAAAAGAAAGAAAAAGAACTCACTTGACAAATCTACCGTGATATTAAGTTTCTCAATTCCATTTATTTCTTACAGGTAAATAACGAACGTTTGTGGGACAATGTAGCTTTTTTAAATAAAGTGAACGCTGAAGACCAAAACGGGGATAAAAACCAGTTTGCCCAAGCAATGGCATCACTCAATAAGCAAATTAACATGTTTTACGTGTTGAATGAAATTACCAACAAGGAACACAATTTGCCAACTAAATACGGTGCTTATTCTGATCAATTAAAAAAGGTCATAAACGAAAACTTTGAAAAGATAGAAAATAAGAAAAAGGAGATAGAGGAAAAACAAAATAAAGAATTTCAGGAAAACAAGAATGGTGCATCAAATAATCAAGACACAAAGCAAGACGCACAAAAAGGTGATACCAATACCCAAAGCACTGAGTTAAAAGCTCGTCAAGCACAACAAGTGCAAAAGGATCAACAAAATGACAGTAAAGGCAATACTGCCACAAACAGTGATACTGGTAAATCGGACAATAAAACCGATACCACTGAAGATGAACAAAACACATATAAACCCTTAACAGCTAGGGAGAAAAAAACCAAAATTAAGGAGCTTACTAAGGACATCTTCTTCTCTACAGCGCAGAACTATTTGTTCTTAGTGCAAGGTGGGTATAACAACAAGTTGTTTGCTGGTGGTTTACCAGAGCTCCACCAACGCTTCTTGTCAGACGTAGTGGTTGATTTCTCTGAGGAAAAACTCGTTTATGAAGTACAAGGTGAACCGATTGTATCCAGTGTAGCCACCATTGTGATTACCCTATTTTTAACAGTTGTTTTATTAGCTATTGCCTTTTTCTTCTTTAAATACCGCAACGTTAAATAGTTATGTCTGTTACACCAATTGTTGAAGTTAAACATTTAGAAAAGGAATTTGGTTTTTGACGCAAAAACCGTATCTTAAAGGATGTGAATTTTGCCATTATGCCCGGTGAGTTCCATGCCTTTATTGGTCAAAATGGTGCGGGGAAAACTACCACCATTAAGTGTTTAATTTCATCTTATCAGCGCTTTAAGGACGAAATTAACATCGATGGCATTAGCAATAAAAACGCCAAGTCTAAGGGTGTAATTAGTTATATCCCTGAATATGCTGTGTTTCCCAAACACCTCAATACCCATGAATACCTCTACACGTTGGGTAAACTGTCAGGTTGTAGTTTGCAAACCATTAAGGAAAAGGTTGATTACTGACTAGCACGTTTTCAAATTGAACACCTCCGCTTTAAAAAGCCAAATGACTTTTCTTCTGGACAAAAGAAAAAGGTTTTATTAATCCAGGCCTTATTTAATGATCCCAAGCTGTTAATCATGGATGAGCCCACTGCTAACTTAGATCCCAAAACACGTAATGAGTTTATGGATGTGTGTTACGAACTTAATGTACGTAACAAGATGGCGGTGTTTGTTTCATCCCATATTTTGGCTGAATTGGAAAACTATTGTGATTCGCTCACTGTTATTCATGAAGGGGAAATTCTTTTTAATGGCAAAACAAAGAACATTGCTAAAGACAACTTTGGTTACCGTTTAAAGGTTAACAATTCCGATAGTTTACGTAAGTGATTAAAAGCACAAAAAATTGCTTACAAGTATTTCCCTGCAACCGATGACTTTCAAGTTGACTTAAAACAAAAGCAATCCAACAAATTTGCAGTTGAGCTTTACCAACAACCTGACTTTGAGGTATTTATTTTTGCACGACAAGGCAACTCGTTGCAAAACATTTACAATAACCTAATTGAGCAATACGAGTACGATCAAGTCCAGCGTGCTGTTGCTATCCGTGAACAAGATGAAGCTGTTTAAAAATTGAAGCGATGTTAACAACGTTAAACAACGGCAACTTTTACTAGCTTACTTCCGTTTTATCTTAAAACAGATCGTTAAATCTTGACTTTTATGAGTTACTGCTGGACTAGTACTGTTTTTACTGGCTTTAGTGCTGTTAATCATCCCCGCTTTCACCAAACAAGATCCTTTGTTTTTGTGATCACACCCAGTGGTGCAAATGTCGAGTTTAATCATTCCTTTCATTGCTCTGTTTGCCACCATTATTACCTTTCAAGTCTTTATTAATGGTTATTACAACGGTCTAGAGATCTTATTAATTACCCGCTTTTTTACTCGCGGTCGGTTATTCTTTGCTCGCTTAGCAGTCTTGTTTATTTGGATTACTGGCACGGCATTTTTAAGTGGTCTTTTTGTTAGTTTAACTGCTACTTTAGGTGCTACATCACAAACAGTTACTGACCTTGTTTTAAGCGTCTTTTTTGGAGTGCTTTTACTTAGTTTATTGTTTAGCTGTGTCTTGATTATTATCGCGCAGTTTTTAAACCGCACCCAAAGTATGTTGTTGTTACTGTTAGGGGTTTCCCTAGCTAGCTTTACAACCGTTATCTTGGCCTTTTCAATTAAGCCACCGAGTGAAAATCTCCGTGATAATGGTTACCAACCACTAAACCTAAGTCTCATCAGTAAGTCTAAGTCCAAGAGTGTCGAAAATGTCTTTACCATCATTGACTCTTCAAATGTATCCGATCCGCGCAATAAAAAGAAATCGATTGTAAAAACCAATCCGCAAGCGATTTGGGATGAATATGGGGAGAGTAGCTTCTTTCAAAGCCAGTATTACTGGAATGTAGGGTATTGAATTAACAGTCTGTTTCGGTTAAACAGCCTCAGTGATTACCGCAACTTTGATGTTAACCTCTATTTTTTAAACACTAAGCTTAATTTTGACAGGGAAGTAGATACTGATAAAATGGTCGACTTTGTTAGCTTTCGCGATCAAAGACACCTTTACTTTTTAAGTTATTCCTTTTTTATCAATACCCATAACCTGCCCACACAACCGTTACCCCGGATTCTTACTTATGACAATAACGGTGCCTTTTTAGAGAAAATAGTCCCTTCCTTTGATGACATAAAACTAGATCCACCCCGGGTCAAAAAGGTTTACAAATTCTTTAGTGACACCATTATCCAGTCCTTCCGTGATTATGAAGCGGAGGAAAAAGAAAGGCAGGAAAAGGAAGAGAAAGAAAAGGCTGAAAAGGACAATGGCAACGGCCAGGATTCCAATAAAGTTAACTCTGTCAGCACGGAGCCTAACAATAAGAATTCATCAGATGCGGATTCTAAGGATAACAATGATTCATCCGATTCCCAAGGCAAAGACTCAAGTAAGAGTAAGCCTAAGTTTAGACCTAGGCTGCCACAATTCTTTGATCGTGTTTCCATTAACTACAGTAAGTTTTCCAGTAGCTTTAACACACAACTAAAACACATTCATCAAGGACTTTCTACCCGTGATGAGGCCATGGAAATCTTTAAAGATAAGGTAGCGTTATTTTATGCCCTATCAATGGCTTATGACAACTTTACTTTCCAAAAGGACAGTGGCATTATTGATAACACTAGCTTAAACAAATTTAAAACAGAGTTTAACAAGAAGCAAAAAGAGCTAACTGAAAAAAACAAGCAAAAACAGGACGGTAAAGACCAAAAGAGTCAAAGAATGACACAAGGTGCTGATAAAGCAGTCACTGTTGAGCCAAAAGCTATGCAGTCTGAAATTGGAATGACTGATCAAACTAATGACCAAAGCAGTTCGGAAACAAAGGACAGCATGGATAGTTCAGACAGTAGTGATACTGTTGATAACACTGATGAATCAGAAGATAAACAAAGTGAAGAGGAGGAAAAGTTCGACGAGGAAATTGAGAATGCCAAAAAAATGCCTAAGGCTGAAGACGCCTTTTTTAATACTGCCTCTATCTGGTTGAGCTCACCATTTCTCTTTTTTGAAAATGGTGCAAAACGCGAACAACGATACGAAATCCATTTGCTCAATTCAAACACACAGGTTAATAACGAAATAATTAAAACTAACAGCTTTTATTACGTTAGTGGTGAACCGATTGTAGGCCAAGAAGTCATTATTGCAATGGTGCTGGTGGTAACACTAGGTCTGTTAGTTGGTAGTTTCTTCGCTTACCAAAAACGCGATATTAAGTAATTAGTTTTTCTGTAACGACCTAACGTACTTCAAGGTAATTTGGTAAGCTAGTTTTAAATCCTTTTTCGCTAGTTTAACCAAAGCGGTAATATCAATGCCTTTAGCTCACTCTTCATGAACGCGCATTGGTTCTAACTTATCCGCTAAGTAGATAATCATGTCTAGTTGACTCATCTTTTGTGGTGGTACAGTGTGGTTTCAAATAGCGCTAAAAACAGCACTATTATTTAAACCATACCAATGCTTTAAAATGTATGCACCTGCATAGCTGTGTAACACCTTTCAGCTAGGGTAATCATTTACACCCGCTACTTTGGCTAATTTCTCTAATTGCGCTTGGGGTAGTTGCTTTGCTAGATCATGGTAAGCACCAGCAGTATAAGCCGTTTTACCATCGAGTTTATTTGCCACTGCTAAAGTTTTAGCTAACTGACCAACGCGCAAACAGTGTTGCATGCGCGGTTCATCTAACAGTGTTTGCAAAATGGTTTTTAGATAAATGTTGTGTTGGTGGATATAGTGCAACACCTTAGCGGGAATTTGTCGAAATTTATGACTACCAAACATTTCACTCGATGCCAAATCTAAAGGACACTTACCCAACAGTCGGACGTTAAACTGTTGTACCAATTGTTCATCAATTGGATAACCGAAACGTTCATAACAGACAAAACGACAAAGTTTTTGTAACTGCTCAATGTTATGTCATTTGGCCAACTCGCTTAATTTATCACTGCCAATTAAAAAGTACAACTCAGCATTGGGAAAGCGCTGCTTAAAGTGTTGTACTGTATTGAACGAAAAACCGTTTTGCGCTTTAATGTCAAAGTTAGATACTGCCATCTGAGGCAATGCTTTTAGCGCAACCCTTAACATTGCTAAACGTGCTTGATTAGATGCTTTAATCGGTGATTTAAAAAAGGCTTTAGCAGTTGGTACAAAGTAAAGCTTTTGTGCTTTAATGGCTTGTACAGCACGCTTAGCAATGTAGATATGCGCTTGGTGGAGGGGATCAAATGCACCACCAAAGATCACTATTTTTTTTCTCAAAGAGCTTAAATTGTTTAATTCTAACTAAAATAATCCTGATAGCTACAGCCATTTGTGCTGTCCAGGTCCATGGTAAAAAAGGAACTAGAAATGTACAACCTCTATACTTTTCAAATAGACCTCGATAAAAAGCTTTTATTTGAAAAAGCAGATAATCAACAGAATTACTCTAAGATTAGAGCTCGTTATTTTAAAAATTCTGCACGTAATCAACAAGCAGTTTTTCTCAATAAAAACCTGATTAAAAACACTTTTAATAAGGCACTGTTAAACTTCTCGGATTTTTTGAGTGGTAGTGGTGTAGAGAGCATTTTTAAGCAGGTCATTGATGATCAACCTGAAGTTTTAAACTACCTCAAACAGGTCAAAAAGGAAGATAGTTGCGATGGTCACAGCGAAGCGTCACAGTTAGTATTTAATGTAGTCATTAACCCTAAAAATACGCTCGCTAACTTCTTTGAGGAATTAACCATTTACCTCCACTTTAATGAAGAGAACAACACAGTTGTCGGGAGCTTTTCGTTAAAGTGAAACATTAAGCGTGCCGACCTGTTTTCAGAAACGAAAAATATTGCCATTAACAATCTGATTCACACCTTCTGTAAAAACAACTTAAACGAGGTTAGCTTTATCCAGATCATTAAATGCTTTGCTAAAACACTGATTAATAAGCAAGGACAAATTGTGTTGGAGAGTTGTGCCTTTAAGCAAAAATGACAAAACATCGTGGAGCAAAAGTACCCTTTTTCCACGATTCACAAAAACTTAAAGATTGTCAATTCTGATTTCTTTGATGCTTTTTTTGTGATCTTGTTGTTAATTTGTCACCTCAATAATAACTTGTTGTGGTTGTGTGAAAAAACTGAGCACTTTGAATGAAAGCTCAACAGCAAGATTAGTAATTTCAAAGAAGATAACACTGAAGTGTACTTGTCGAAAATGCTGTTGTTTTTAAAGGACTGGTACTTTGAAAACCAAGCCGTTACTAATGAAGACATTGAAAAGGTTGATGAAGTGGAAGACATTGGTAAGTTAGTGGAAAAATACAGCGCTAATCAACCACAAAAGTTGTCTTCTAATTCAACCGTTTATGTATTTGAACCAGACAAAAAACAGTGCTTTTTAAAGAATGATGACTTTTTCAACACTAACGAAGCTAAGCTGTTGTTTTTAATCACAATGCAACCCAATGTTTTTGGTCTGGATGATACTGCCATTGCCAATGACTTAAACCTAAGGGAAATTGGTGACTTCTTTAAGGAAATTGACTTTACTGACAGCGATGTGCTCAATGACTTTCAACAACAAAAAGAAACTTTGTTAGTACGGCGTACCTTTAACCAACTGTTGTTTATGAACAGTAATACCGATGTTTTGAGTATTGTTAACAATAAGTTCAAATCAGCGATCCATAACATTGTCTGAACGATTACTTACAGTAAGGCAATTATGCTCAAGGCATTTGATTACTCCAAGATCTTTGAGCAAAACCGCACTAACGATCCTTCTCTCTTGCGTTCTAACCTCAACAGCATTAACCGGCTGCGGTATCTCAGTGAATACTTCCGTACCGCTTCAGTTAAGTATGACCAGCTGTACACCAAGGTAAAGGAGTACATGCAATTAGATCAGTTCTTAGTAGACATGATTAACCAGGTCAACCATGAAGATGAGATCTTTGGTAAATACAAGGAACGGGTTTATTTGAGTTTGGGAATAGTTACTGCTGTTGTCTTTGGTATTATTGAGTTCTTTAACTGTGTTTGAACGGTTTTAACAGTCAGTCAACAAACGGCTGAAAAAAGTTTAGCTGATCCAAGAAACACGGTAATTATTGGTATTGGTACTATCCTTGTTTTAACACTGTTAATTACCATTTTAACCTTTATGACAAGACGGCTTTACCTGTTTGAATTTAACAAGAAGCATAAATAAAGATGGAATTCAATAAGTTACAACTCAGTCACTGTATTTCCTTTTACATTTCAGACGTTTCGGAAGTGTTCTTTGAAAGTATTAACCAACACCCTAGTCGTGATTTTGTCAACAACATCCTGCAAAAGATTAAGAGCACTCTCAGTGAAGAGGAATTGGAAAAGCTCAACACCATTGAGGAAGTTACTAAGGATGAGAAAATTTTGATCATGCTCAACCATGTGTTGAAGAAGATTGTTTCTAAAACGGGTAGTTCTAGCTGTGACTTATTTCAAATAGTGAAACACGACCGCTTTAACGAGCCGGTTTACATCCAAAGTGTCAATGCCTTTGAAAACAATTTGATTAACAATGAATTTGCGGAAAGGCGGTATGACTATTTAATTGAAATTAATAAACACTCTTATTTAAGGAAGTATGTCAATGCCATTCGCATCTCCTTCTTTTTAGATTTAAGGGCGCAAATCTTGTCAGGTTCCTTTACTTTGGATGTGATTAACAAACAGTTGGAACACCAAAATAAGGAAGAGTTGTTCCAAGCGATTTACTTACGCTCCTTAATTAAGCATTTTATCTCGAACCAACTTTACCCGATTTCCTTAAATTCGTTTATTTTTGGTGATAAAAACCGTGAAAACAAGACGGTTGAAAACGACAAGCTTAGTGTGCTTAAAAACAATTGGAATCAAATCTTCTTTTCTAAGTACTTTGATTTTGTTGCCAAAAACAAAGAAGAGCGTGTTGTCGATAACAACTGCGATGAGCTGTTTTACGCCACCATGAACACGCTCTTGATTATGTTGATCATTATTGAAGAGCTCCGGGTATACTTCAACAGTAAAGAGCCAGCCTTAATCTTAAAGCTACTAGATAACAAGGTTTCACTGCGCGAAGACCCCGACCAAAATCCAGAAACTGATTTGCATGAGTTAATTAAGTTTGCTGAAAAGAATTACCTCGAAAAGGAAAAAACAAGTCGCTGACACAAAAAACGCGTCAAAAGCTTAGAAGAGTTACTAGAAGAAATTAAACAAATTAATTTAGAGACTAAAAACGAATCGTTAGCATACCCCGATGAAATTGTGGAATTAGAGTTAGATAACGTTCACAACTTTGTCTCTACTAAACAGGTTTTTCGTCACCAGTTAGACTTACAAACTTTACATGGTATAGTCATCAACCCTGAGCGCTATGGTATTGGCATGTGGAGTAACCACTTTGTCGATTGGGAGGAGTTTAAAGATTTAATTGAACAGATTACTGATGCTGAAAATGGTAGTGATCTCTATGGTTTTGAAAAGGACTTGGACGAGAGCATTTGCCAAGTTAACAAGAAGTACTTAACCTTTATTAGTAGTGACAGTAGCTCTTTCTTAATTATTAAGAATGACCAAACTAAGGTTATCTCTAATTATGTCTGGGCGCAACTGTACTTTGAAACGCGCCGGTGGATTATTAATGATATTGAATACGATCTGTATGAAAAGGGGTTTGACAAGAGCCACTTTGCTAGTAACATTGCCCTTTTAGAGAGCTTGAATTTTAACTGACTCGATCCCTTTTACGGATTGACCTCAATCAAGGAGATCATGCAAAAGATTGACAGTAAGAGTAATTTAAAAACATCGATTGCCGAAATGGTCGCTAAGTTTAAACACGAACAGCGCATCTCCAAAAAGGACAATGAGCGGGTGTTGATGATCTTTGCTTATGTAGCCGCTGCGGTAGTTGGTTTTATTAACTTTTTTTCGATGGTGTTTACCATTCTTACAGTCAGTGATCTCAATGCTGGTCTCACCCCCGCTAACATTGTTGTTATTGCAATTGCGAGCTTATTGGCCTTATTCTTAATTGTTATTGCTGTTTTATTTCGGTTTAGGTGAAAGTACATTAAGCACTAATGCAGGTTAAAGTAATTGATGAAAACAGCAATACAACCGCTGTCCTTAACTGCGCTAAAGCGAAAACGCGAGCGCTAGCTTGGTTGTGTGATACAGTACTGTTAGCGATCTTACTCGCAATTATTTACGGCATTAGTAGCATTTTCATCAAAGAACAGTCCAGTGTGTTTTTAATTATGACGGTAAGTCAAGCAGTCTTGTGGTTGACTTACTTTGTCATTTTACCGGGTTTATGGAAGGGCAAAACGCTCTTTCGTGCTTTACTGGGTTTAAGTTTACTCATCTTTAAAAAACGCTTTTGAAACCTCTTAGTGCACGAGCTTTTTCTGTGAGTATGGTACTCAGTTATCTTTCTAGCACTAGCAATTTACTTTTTTGTCAACAGGGATGATCCGAAGATACTGCAAGCTTTTTTTGATAACCAAAACAGCAATCTTAGCTGGATATTTGTCAAAATCTTGCTAAGTGTTATTAGTGTCTTACAACTAGTATTTGTGGTGTATTTCTGTTTCAGCTCCCAAAAGCAAGCGTTGCAAGATTTGTTATCTAAGAGTTTTATGGTGCAAAAAGCAATTAAAGTAAAGGATTGTAAAAGTGAATTGAAGTCCACTAACACTATAAAAACGCACTCTGATTTACCCGGCGACATTGATTTAGAACAATTAGGTGATTAAGTTATGGAACATTTAAATCAGGAACAGAAAGCTGCTGTTACTTGTGATAACGGTGTTAACGTGGTGTATTCCGGTGCTGGCACGGGTAAAACAACCGTTATTGCTGAACGCTTTGCTTACTTAGTCAATGAAAAAGGCGTTAATCCTCAAAGTATTTTGGCCTTTACCTTCACTGATAAGGCAGCGAGTGAAATGCGCCAACGCATTATTAAGTTAATTCCACAAAAAAGCTTGCAGGATCTGCACATCTATACCTTTCACAGTTTTGCCAACAGGTTTTTGCAAAAACATGGCAAAAGTGACTTTGCCATCTTGAGCGATTCTAACCGCTTCTTTAGTGATTATGAAATGGGTGATCAACTACAAACAGTGGTGGAAATTTATAAAAACAAAGTAGTTGATTTAGAACTTGACAACCTAGAGTATAACTCCGCTTTTAGAGATGCTTGTACAGATACTTTTAACGAGGATTTTAGTACCATCTCCAATGGTCAGTTTCGTAAACGGGCTGCGACTGCTTTACGGGCTTACCAGAACTATTTAATTACCAATAACCTATTTGATTTTAGCGATTTAATTATTGAAACTTGCCACCTTTTAAAAGGTAATAGTGAATTGTTGCAAGCATTTACCGAAAGTGTGCATTATATCTTAGTAGATGAATTTCAAGACACTAACCTAGCCCAGTACGAATTGGTAAAGTTACTAGCTACAACTCATCCTAATCTCTTTTTGGTGGGTGATAGCAACCAGATGATTTACGGTTGGCGGGGTGCTGTTGTAGAGATCTTTGAACTTTTAAAAAATGATTTTCAAGCAGTCAAAGAGTTTTATACCACCCAAAACTACCGCAGCATCCAAGCGGTGTTAGCAGTAGCGAATGATGTACTAACCGCAATTGCTAGAAAAGAGAGGAAAGCGCTCGTGTTGTTGCACTCTAGCATTGATTCTAAAGCTGTACCGGTACACTATAAGGCCAATTCTTTGAAAAACCAAGACCAGTGGATTATTTACCAAATGAAACAGTTGCATTTAAACAATGGTGTACCTTATGATCAGATGGCGGTGTTATTTCGTAAGAATAAACACCTTGACGCTTTTAGTCAAACAGTGCTGGAAGATGGTGATTTACCTTTAGCTAAATTAAACCTTTTAACAATTCACGCTGCCAAGGGTTTGGAATTTGAGGCAGTATTTGTTTATGGCTTGGTTGAACGCGCTTTTCCTAGTTTGCACTGGGATGGAAGTGATAAGCACAAGTTACTAGAAGAAATGAAACTGTTTTACGTAGCGATTACTCGTGCTAAACAGTTTTTATTTTTAGTTTCTGTTAGTGTGGAAAGTTTCAATGCGTATTATGAGCCGTCACGCTTTTTAAAACTAATTGAAAAGGAACATTTGCAAACACAAAAGGCAGCGTACTTTAAAGAACAATTAAAGACACAGCCAAAACCAGTTAATTTGTATACAGAGACAGAAAACGCGGAAAACTTACAAAAAGCCACTGACTCTAAAAAGTGAATTATTTTAGGGGCGATTCTTTTGATAATTGTGATTATTACCGCTGTGTTAAAACTTTTTGTTGAAAATTAACATTTTATCCCTTTTTTATAACAGATTACACTAATTAAAAAAACAATCTTAAACCTAGTACTATGGCATTTAACATTAGCACTCAACTCAATAAGGAACAACGAGCCGCTGTTACCTGTGGTAAGGGTGTCAACATTGTCTATTCTGGTGCTGGTACTGGTAAGACCACCATTATTTCCCAACGCTTTGCTTATTTATTTAACCAAAAGCGAATTAATCCTAGCAACATTTTGGCGTTAACCTATACAAGGAAAGCGGCGAGTGAGATGAAGCAGCGGATTTTGGAGCTGTTACCGGAAAAGTATCATAAGGATGTCAACATTTACACCTTCCACAGCTTTTGTAGCCGTTTTTTACGTGAAGAAGGGCAAAAAAACTTTGTGATTGATGATGACTTAAGTAACTTTCTAAAAGACTTTCTCAAAGAGAGTGAACTAAAAAGTCAAAAGGTATTACAAATTATTGACGGTTTTAAAAACGCTTACTTTGATTTTGATACCAACAGCTTAAAGGATGATGAAAGATTGGTCGAGTTGTGTGAGTTTCATTTAGACCCCCAAGAGCGTAACTTTCAACTTTTCAAAGAAACCGCTATTGCTGCTTTTGTTGAATATGAAAAAGGTAAACAACAGAACAATAAAATTGACTTTGCTGATTTATTAATTAAAACTTGCACTTTACTGAGCAAAGATCACAAATTATTGCGCAAGTGATCGAAAAAATTTCAGTACATTTTAGGTGATGAGTTTCAAGATACCAACCAAATTCAGTATGAACTAATTAAGATGTTGGCCAGCCATCACCAAAACCTTTTTTTAGTGGGTGACAATAACCAGATGATTTACCGCTGAAGGGGCGCGGTTAGTGACATTATTGATTCTTTAAAAAGTGATTTTAAGGTAAGACCCGAAAACGAGTTTTACATTACCCAAAACTACCGTTGTGATCAGAACATCTTAACGGTGGCAAACTCGATTCTAGGCACCATTTATGCCAAAGAAAACCAACCTGATTCAACTAAAGGCTTTCTTTTTTCTGCCATTAAATCAAACCGGCTTCCAGTTTATTTCCAAGCCTCTTCAGTTGAAGGCCAACATTCTTGAATTATCAATAAGATTAAGAATTTACATAAAAACCATGGCATTCAATACAAAGACATGGCTATTTTGTTTCGCACCAACAGAAACATGGATTCGATGACTGAAGCGCTTGAAGCTGATGGCAGTATTCCCCTTAAGCAAAATAAGGGTTTTTTTAAACAGTTAGAGACTTTTAAAAAGGTATTAGTGGCACTCATAACTAGGTCAAACTATGACATTAAACTAGCTTTAAAAAGTTTGCGGGTTTGACCTAATGTTTTAAATAAAAGTCTCACTGTAAACGAGCAAGTGAATTTAGATAAAATCTTGCAAAACTTAGAGCAAGCCATTTTTTTAGATGAGCATACTCGGGGTGAGTTGACGGAAGCTGCTAAAGTTTTTACGCGTTTAATTAAGTTTGTTGAAGAACAACAGTTTGAAGCTTTATTAGCCTTTACTTTTGAGGCATTAAATACTGATCAGTTTGTCTGCAACTTTATTTTTAGAACATTGCAAAAACTGCAAACTGAAAATAAAAACTTCACCATTACCGATTTTATTAACGAGTTAAGGTTCCAACAAGATGAGTTAAAGCAGTCCAAAGATAATGTGATTAATTTAATTACAGTGCACTCTGCTAAGGGCTTGGAATTTGAAGCTGTCTTTATTTATGGGATCAATCAAGACAACTTTCCGCTCAAATCGAAGAATCAAATAATAGATCTACAAAGAGAATTGGATGAACTACGCCTTTTTTATGTGGCCTTGACAAGGGCAAAGAAATACTTGTTTTTACTTTCAGTTTATCAAATGAGTGGTGAAATTATTTACCCCTCTAAATTCATTCGTTTTATCAATAAAGAGGATAGACTAGAAATTGCCACCATTAACCATAAAGTTCACCAGGATGATGAATTCTTCGATTCTTCTAAAACAGAAGACTATCAAAAAAAGTATTTAACAGAAAATACGGATTTTGTTAATGGTGACTCTGTTAGCCACCGTACTTATGGTAAGGGCGTTGTTGTTGAAGTTAGAGAAGATGCAGTCTGTGTCGCTTTTAAAAACAGTAAGTACGGACAGAGGTGAATTGTTAAAAATCACCGTGATTTGGTGAAGGCTGTGTATTAGTTATGATCTGCTATTAAAAAAGAAATTGTAATAATAAAAATGAACAAGTTTTTACCAATTTAATAACTAAAATTAAAATCCTTATTAATTGTTAGTAATAACAACTTTCAACATAACTAAATAACTAAACTCACTATATCATGGAAAAGAAACGTACTGAACAACGTAATGGCGTTGAAAAGAAGATTTGAGAAATAGCTGATAAATTAAGGGGAACCATTGATGGATGGGACTTTAAAAGCTATGTTTTAATTGGTCTTTTTTACCGTTTTCTTTCCGAAAACCTATGCAAATATTTTAACGATTCTGAAAGAAGGAATAATCCTGATTTTAGTTACGAAAATTTAACAGATGATTATGAAGCAATTGATGCATTAAAAGATGCTGCCATTGCAAGTAAAGGCTTTTTTATTAAGCCATCTCAGCTTTTTCAAAATGTTGTTAAAAGTATTCGTGAAAATAAAAATAACGAAGATCTAAATACAACTTTGCGCGATATCTTCGATGATATTGAAAAGTCTACTGAACTAGGTGATGGAAGAAGTAAAGAATCCTTTAAAGGTCTTTTCAAAGACTTTAATGTTTCTGAGGTTAAGTTAGGTTCTACTTTAACGATCCGAACAGAGAAATTAAAAGAACTTTTAACAAGCATTGACACAATGGAGCTCGATGAATTTGAGAAAAATTCTATCGATGCATTTGGTGATGCATATGAGTTTTTAATTTCCATGTATGCACAAAATGCTGGAAAAAGTGGGGGTGAGTTTTTTACACCCCAAGATATATCTGAACTTCTCGCTAGAATTGCGATTGGCAAAAAGGACACCGTTGATGATGTTTATGACATGGCTTGTGGCAGTGGTTCTTTACTTTTACAAGTAATTAAAGTTTTAGGTAAAGAAAAAACTTCACTGGTTAGCTATTACGGTCAGGAAATAAACCACACAACTTACAACCTTTGTCGGATGAATATGATTCTCCACAATATTGATTACGCTAATTTCAATATTATTAACGCTGATACTTTAACAACGAAGGAGTGGGAGAAGCATTACGTAAATTGCTCAAATGAAAATGGGTTTGAAGTTGTGGTTTCCAATCCACCATATTCCATTAGTTGAGCTGGTGATAAAAAATCCAATTTAGTAAGTGACGTTCGTTTTAAAGATGCAGGGACATTGGCACCTAATTCAAAAGCTGATTTAGCTTTTGTTTTACATGCTCTATATGTATTAGGTCAAGAAGGTACAGCAGCAATTGTTTGTTTCCCTGGAATCTTGTACCGTGAAGGTAAAGAGCAAACCATTAGAAAATACCTAGTTGATCAAAACTTTGTTGATGCAGTGATCCAATTACCAAGTAATCTTTTTAGCACAACATCAATCGCTACTTCAATTCTTGTTCTCAAGAAAAATCGTGATAAAAAAGATCCCATTTTCTTCATTGATGGTTCTAACGAATTTGTTAGAGAAAAGAAGAATAACCGCTTAAGTCCAAAAAATATTGAAAAGATTGTTGATTGCTTTAATAGCAAAAAAAGAAGAAGCTAATTTTGCAAAGTCGGTTGAAAGGGACAAAATTAGGGAATCTAACTACGACCTTACAGTAGGTAAATACGTAAACTCAGAGGCTGAAAAGGAAGAATTAGATATTAAGGTTTTGAATCACTCTATTGATGAAATAGTAGATAAACAAAAAGATCTAAGAACTAAAATTAAGGATATTATTCAGGATATCAAAGTAGATTTTGACAACATTGATATTAATAACTAGTTGATAATAAAGCAATTCTTTGTTAATTTACAAGAATTTAAAAACTAATAAATTAATTTCATTATTGTTGTGCAAATTAAAACTTACAAAATCAAGGATATTTGCGAGATCTCAAGAGGCAAAGCAATTACTAAAAAATACATTAAGGATAATCCTGGTCAATATCCGGTTTATTCATCTACTACTGCTAATAACGGTGAAATTGGCAGAATAAAGGATTACGATCTTGATGGCGAATACGTTACCTGAACTACAGATGGTATATATGCAGGTACTGTTTTTTATCGTAACGAAAAGTTTAATGCTTCTCAGCACTGCGGTGTTTTAAAACTTAAAAATAACGAAATAAGTGCTAAGTTTTTAACTTATGCATTAGGAATGGAGGCTCCTAAATTTGTTAATAATGCTTGTCCTATTCCTAACCTCAATTTATCAAGAACTGAGGAAATTGAATTAGATTTCCCACCCTTGCAAATTCAGCAGAAAATTGCTACTATCTTAGATACTTTTACCGAACTAAGCGCCGAACTAAGCGCCGAACTAAGCGCCGAACTAAGCGCCGAACTAAGCGCCGAACTAAGCGCCGAACTAAGCGCCGAACTAAGCGCCGAACTAAGCGCCGAACTAAGCGCCGAACTAAGCGCCGAACTAAGCGCCGAGCTAAGAGAGCGAAAGAAGCAGTATGCCTTCTATCGCGATTATCTGCTCAACCAAGAAAATATCCGAAAAATTTACGGAGCAAATATACCTTTCGAAACTTTTCAAGTAAAGGATATTTGTGAAATTAGAAGAGGTCGAGCGATAACTAAAGCGTATATTAGGAATAATCCTGGAGAAAATCCAGTTTATTCCGCAGCTACCACAAATGATGGTGAGTTAGGACGTATTAAAGATTGCGATTTTGATGGTGAATACATCACTTGAACTACAAATGGTTATGCTGGAGTAGTTTTTTATCGTAATGGCAAGTTTAATGCATCACAAGATTGCGGTGTTTTAAAGGTTAAAAATAAAAAAATCTGCACCAAATTCCTATCCTTTTTATTAAAGATAGAAGCACCAAAATTCGTTCATAATCTAGCTTCAAGACCAAAACTAAGCCAAAAAGTAATGGCTGAAATTGAACTCTCTTTTCCACCTCTAGAAATTCAAGAAAAGATTGCTGATATTCTCTTTGCCTTTGAAAAACTTTGCAATGATTTAGTAGAAGGTATTCCTGCCGAAATTGAATTGCGTAAAAAGCAATTGGATTACTATCAGAATTTCTTATTTAATTGAGTGCAAGAACAAAAGAAGAACTCACTTTCTACTAATCTAAATTAAAGGTTATTTCTTAACCTTTGAGAGTTTCTTTAATTAAAAAAGAAATTTCTTAATTTTGTTGGCATTTCTAATTAAGCCGTTCAATTTATTTAGTGTAAAGAGCGCATATGCATTACACATATAAATTGAAAATCGGGCACTTGTTAAATTTAGCTCAATTCTTATACAAAATTGAACGTTTTAGTTATTTTTTAGATTCTCCAAAGAAAGGAGGGATTTTTTATGCCTAAAATTATTTCTAAACGAAAATTCAACATTCTTAGAAAAACAAAAGAAGAAATCTTTTCTCAAATTGTTTACACTAAGAAGAGAAACGGTCTGCACAAGGCTGCCAAAGAATATTACTTCAAGGACAAAGACTTTACAATAATAGAAGATCAACAGGATCGACCTGATAAACCTGAGGAACTGGACACACCGGATATTCCAAAGCCACCAAAACCTCCCAAGGGTCCTGATCAACCCGAGGAACCAGGCCAACCAGGTGGTCCCGATGATCCTAATTCAGGAAATAAAAAGATGCCAAAGCCAGACGAATTTGTAACCCACAGACAACTTCAAGAATTTAAGAAGGATCTTTTAGTGGAACTTCACGAAATCTTTCCTACAAAACCTGAATTAAAACGGGTTGAAGAAAAAGTTGATGTTCTGTTTGAACTTCAAAAAGCTCAGGGTGAACAAATAAGAATCCAAGGTGAACAAATCAAAGAACTGAAAGTGGAGCAAAAAGCCCAGGGCGAAACCCTACAGCTAATCTTGCAAACACTACAAAAGATGAACGATCGTCTGGATAAGATCGAAGGTAAAATGGACAAAATGGAAAGTAGAATGGATAAAATGGAAACTCGCTTAGACAAACTAGAGTCCAAATAAATCTAATTGAAATAAGAATAACAGCAGTTAATTTTTAGGTTAGCACGTTGCTAATTTATTCATAAAAGAAGAACTATTATTAGTTATTTATTCAGCTTCTTCAGTGGTCTTTAAAGAAATATTACTATTTAATAAAAAAATTTATTGTTGTTAAAGTTTAAACTCTAGTATTTCTTATTTATCATTAAGTTATTTAATTAACATTAAGTTATTTAATTAACTTATTTAACTTAATCTTCACATGGAAAAACTGGATTTTTTAAAGATAAAAAAAGATTGAACTGTACCAGTTGAATATCAACCTAGTGATCAAAAAATAGAGTGACAATCTTCAAGAAAAATAGTTCAAAATTTAAAAAACAAACTTTTATCCTTGGGTTATGAAGATATTAGCAAGGGTTTTGACGAAATTCCTAAATTCTTAAGAGAAAAATTTAAGAAATCAACTTAGCTTATTAAACGATTTTCAATTTTCCGATAGAGAGTGATCAGATTTTTTAAATAACTTTATTTTTCAATTAGGTGACGGCAAAAAAGGAAGAATTGAAAAAATCCAAAAAGGTGAGCTTTATGAACTAAAAGATGAAAATGACAGAATCATTAAAAATGTTCGTTTAGTAAATAAAAGAGAACTATCTAAAAATAAATTGCAATTTATTTGCCAAGATGAAAGGAATGAGGTTATTATATTCATCAATGGCCTTCCTTTTGTTTTAATAAGTATCTTTGAATTTAATTCACCACTTCAATCAAGCTTTCAAGCATGAAAAATTATTAATTCCCTAATAGATGACGAAAAATTTAAAAAGTTTTCCGATTACTTGCACCTTTTTGTTTGAACAAACGCAAAGGAAACAAGATACTGCTCAATAACAACATGGGATAACCTTAAAGAAAAAAGGAAATCAGTAAGAGGATTGTCAAACTCTTCTTTTAAAAGTTTGATGAGATGAAGTGACTCTGAAAACAATAAAATCGACTATATTGAAGACTTTGCAACTCATTTTTTAAACAAAAACGCCTTATTAAACGTTATTTGTAAATTTTGTGTGTTCAGGTCAAATAGTGATCTTTGAGTTATGCGTCCTTACCAAATATGCGCAACTGAAAGAATTTTAGAAAAAATAAAAGAAGACAATAGGAACAGTAAAAACTCTAAAAATGCAAGTAAGGGCGGCTGTATTTGACACTCAACCGGAAGTGGTAAAACACTAACTTCTTTTAAAGCTGTTCAATTGGCTAGTGAAATAGACTTTGTTGATAAAGTCCTTTTCGTTGTTGACAGGAAAGACTTGGACAACCAAACGATCGAAGAATATGAAAAGTTTCAAGCGGGTTCTGTTAGCGAAACTGAGAACACAAATGATCTTAAAGAAAAGATATTAGACGATAGTACCGCCACACGTGCAATTGTTACTACTATTCATAAACTTAAGAGGTTAATAGATCAGAGAAGTAAGCTTAAAGACGAAGATCTTAAAAAGAAAAACATCGTTCTTATATTCGATGAATGTCATCGATCTCAATTTGGAAAAATGAAACAAGAAATCGATGAGTTTTTTTAAAATGGGATTTTATTTGGCTTTACTGGAACGCCTATTTTTGAAAGTGAATATTCAGACAAGAATCAAACTCTAGAGAACCAGCAAAAGACAACTAGAAGTAAATTTGGTGAAATTATTCATACCTATACAATGTCTGATGCTTTGGATGACGAAAATGTACTTTGTTTTAGGTATAAGAGTTATTACATTAAAGATGATGGAAGCCGAATTGATACATTAAATGAAGAGAGAATTAAGCATATTGTTGATTTCACTTTAGATAAATATCTAGATGCAACACAAAATACTCCGAAATTTGATAATTTTGAAAATCCTGTAAAAGAAGGGTTTAATTCGATTTTTGCCTGTGAGAGTAAAGAAGCAGCAATCGAATACTATAAAGAATTTAAAAAACAAATTGAACAAAAGAAGCTACCAATTAAAATTGCAAGCATTATAGCGCAAACGAAGGTAGTGATGAACAAGAAATTAGTGTAAAAGGTCTTGATAAATCCTCAAAGAGTTTTCTAAGTCAAATCATTTCTGATTACAACGAAACCTTTCAAACAAACTTTAGCATTAACAATCTAGATGAATATTACAAAAATGTTTCTGATAGATTAAAGCCAAAAAAGACAAAAAATTTAGATGGTGAAAATAAAAACACAAATGGCTGAGAAATCGACATTTTAATTGTTGTTAACATGTTCTTAACTGGTTTTGATGCACCAACCCTTAATACACTTTGAGTAGATAAGAATTTAAGCGAACACACTTTAATCCAAGCTTTTTCAAGAACTAATAGATGTTTTGGAGAAACTAAAAAATTCGGAAACATTTTTAGCTTTAGAGATATAAGAGAAAACTTCGATCAAGCTCTAAAAATGTACGCAGACAAGCGTGGTCTTAAGGATATAAAATCCAACATGGTTATGCGAGAATTTGAGGAAGTTTTTGAAAAATTCAAAGATTGAACCGAGAAAATAAAACAAAAATACCCAAATCCAGATGAAATTCTTAATTTAGAAAAAGAAGAAAAGTGAGATTTTTCTGATCTTTTTGGTGAATTTCTTGGAGATTACAGAACTGTAAAAAGCTATCATGAATTCGACAAAGACAACCCTTATCTTCCAAGTGATGAATTCAATAAATATTTTGCTGCGTTTTCTCAAATTCAAGAAGAGATAAGGAAAGAAAAGGCAGAGAAAAAAGCGCTAGAAGTACAAGAAGAAGATTTATCAAATGAAGTACGAAAAGAACCGCAAATCTTTTCTTGAGAATATGGTGAGGGTATTCAAGCCAATTTAGATTACATTTTTCATCTAATTAAGGATCTCTATAATTGTGATAATGAGCAAGATAGACAAAAAGTATTAGACAAAATTAAGACTGCAATTAGAACAAATCCAGAGATTCAAGTGAATGAAGATATTCTTAATGAATTTATAGAGGACTTAGGGAAGTCGTTCGAGAAAGAAAAACTTGAAGAAGTTTGAAAAACAGAAGGTAAATTACGTGAGTGTTTAAAGGATTTCTGTAAAAAGAAGGAAGAAAGCAATAAGAAAAAAATTATTAATGACTTTTTAGTTAAAGGAGTTTTCCTTGTTGAATGAGTAGCTGATAAACAACTAAATACAGCTAAAAAGAAAGGTGAATACAGTCCTTCTTGATCTGATACAGAAAAATTAATTTGAAAAGAAAAATCAACCAATCTTCCAACTATGACAGAAAAAGATGAAGATGGCAATTTCAAGTGAAGAAAATTAATTACAGATATCTCGGAAGCTTTTAAAAATCACTTCGAAAGATTTATCCGCTTTGTTGAAATTAGATAGTTTTAAATTATTATCTATAAATCAGGTTTTCAACCATAGATTACTATGGAAAAGAAAAATAAAAAAAATACCGCAACCGTTCCTACCAAAGAACAATTGGATGGCATGTTTAAATCTCTTGAAAAAGAAGAGAACTATGATGTCTTTGTTCAAGTCATAAAAGCTTTAGGTAAGATGTAGTTGTGCGTGAAATAAACGTTTATTATTTAAGCAACGACTCAAAAAGTGATAAAGACGATATCGATTATTTAATAGAAGAACTTTTAGTTGAAGGTTCTCGTTTTAAAAATAGTCTTGTTACGTTTTTAGAAAAAGAAAAAGACTGCCAATCTTGCATTTGTTTATTTAAATCTAAGCTTTCTTCTTCATTCTAAATAAGAGTCAAATTTAATGAATTAAATTTAGATGCAAGTGATGAAAAAACTAAGGAATTTTTAAGATACATAGTTAGTAAATCAATAAACAATGCAAAATTCCTATCTTCTGAAGACTTTGATGACAAAAAAGAAGACGAAATTCTAAGCATCATTGAGAATACCTTTAATAGTTATTGCTACAAAAGCGATTTACTAAGTCCAAGTTTGTATTGTAAAGAAGGCCGCTTGAACATAATTGATTTAATTAACGATATTTTTTGCAAGGTTTTAACTTTTCATAAATTGTCGAACGGAAATAAAAGACTAGCTACAGCCTTATTAGTTAATTTTCTTTACACTTTTGGATATTATTTCAAATGAACAATACCTCTTAAAAAAGAGGAATACTTTAAGTATGCTCACATGATCAAAAACTTTGTAGTTAGTTTTGAAGAACATAAAAGAGAAAACAGGGATGAATTTCTAGCAAAGCAAACAAAGAAATTTATCATGGACAACATCCTTTTAGCTCTCAATTTTTGAGAGACTAAATTAAATTAGTTTTTCTAAAAAACAACAATTAGCTAAGAAAAGTTATATGGACAAAAATGCCTTAAGAAAACAAATTCTGCAAAAAAGAATGGCATTAAGTACCATTGAAAAAAGTCACTTAGATCAAAAGATTAATCAAAAATTAGTTGCTTTTTTAACTCCTAAGCCATGCATTAAAACAATTGCACTTTATGAACCCATTAAAAATGAGGTTACTTTTGTTGACTTCTTCTTTGAGTTTTTAAAGATTAACCAAATAAGAGCTGTTTACCCCAAGGTAATAAGTGATACCGAAATTATCTTTATTGATCAGGAGACAAATACATTCGAACCTAATCAAATAGACTGCTTCCTAATACCATTGGTAGGCTTTAATAAAGACAATTACCGTCTAGGCTTTGGCAAGGGCTATTATGACCGTTATTTAATGCAATTAACTAGACAACAACCTAAAATAGGAATAGCGTACAGTTTCCAAAAAGGTGATTTTTTAGCCGATCCTTGGGATGTACAACTAGACTTAATTATTAATGATGAATAGCATTAAATTCATTTTCCTAGGTGACGTCTATGGCAAGGCCGGACGTAATATTATTAAGAATAATCTAGCCCAACTCAAATCAAAGTATCAAGCTGATTTAGTCATTGTTAATGCCGAAAATACGACTCACGGTAAAGGACTTAGTTTAAAGCACTATGAGTTTCTAAAAGAGGCAGGCGTTAATTACATTACTATGGGTAATCATACCTGGTTTCAGAAGCTAGATTTAGCTGTTGTAATTAATAAAAAGGACTTAGTGCGTCCTTTAAATTTAGACACCAGTTTTGCTTTTCATAACTTAGGGCAAGGTAGTCTTGTATTTGAGTTTAATAAAGCAAAGATTAGGATAACTAATTTACTGGGTACTAGTGTGCCCTTACCCTTTAAAACAACTAATCCCTTTAAAGTTTTAAAAGAACTAATTTTAAAGCGCGATTGTGATCTTCATATAGTTGATTTTCACGCTGAAACCACCAGTGAAAAAAACGCCTTTTGCATGGCTTTTGATGGTTATGTCACAACCATTTTCGGCACCCACACCCACGTGCCTAGTGCTGATTTAAGAATCACCCCCAAAGGTAGTGCTTACATTACCGATGTCGGGATGTGTGGTCCGGGATTTGGTAGTGTTATTGGTGCTAACCCCGAACAGTCGATCAGACTCTTTTGTGCTGGTAGCAGAGAACACTTTGAGGTATCTAAATGCGGAGCACAACTCAATGGTGTCTTCTTTGAGGTTGATGTAAATACAAAAAAGGTGATTAAAACAGAGGCGATTAGGATTGTGGAGGACGATCCTAGATACTTAAAACAAGACTACTTTAACCTAATTTAACTGGGTTACCTTATTTTCTGTTTTATTCAATAAACGGTGAATGTTTTTGCGGTGCAATCAAAACACCGCAATTGTTAGTGGTCAATACCACAATACAAAGAAGAGAATAATGTACCAATCATTTTGGTAAGAGATCGCGTTTATTGGATTAGGCTTAAAAAAGTAAAGGTAATCTAACCAAGGCACTAGGATAATGATCGCTAAAATAAAGAAGGTAACTAAGCTAGCTAAAGAAACGTACTTGGTTATTAAAGTAACTAGCAATCACAAAACTAAGCAAATAACAAATCACCACAGCGAAATTGCCAGCAAAGAACCGCCTGTAGTAGCGATGGCTTTACCACCTTTAAACTTAAAGTACAGTGGAAAAATATGACCAATGGTAGCTGCAAAACAACTTAAATAAGTGAGGTAATAAACCTTTTGATACTGGTTTACATTAAGGTATTGTTGTAACCAAAAGCTAAAGATTAAAAAGCTGAAAACAAACGCCAAAAACCCCTTTAAAGCATCACATAAACCAACTAAGAAGCCTATTTTCAAACCAAAAACACGGATTGAGTTAGTAGCCCCCGGATTCTTGGAGCCCAATTTCCTGACATCTTTGTTTAAGATCTTGCCAAAAACATCAGCAAACATCACTGAACCCATTAAATAGCCAATAACAAGGCTAAAGACAATAAGTAGAGCGATGGCGCTAGCTGCGTTCATATGCTTTAAATAATTTTATTTAATACATGGATTACTTGACTATCAAACTGGCTTGGATTTAACCGTGCTGTAAAGTTTTTTAGCAAGCCGTTGTAGTATTTCACTAACATTTGTTTATCAGTAAAGTTAACCAAATGCGGACCCAAGATCATTTGTTCTTCAGTTAAATACTGGGTTTGCGCCTTTGTTTTATCCACAGCTAAAACTAAGTAAATAAAACCACGTTCTTCAACTAGTGTTTCAGTGGTAATAGTTCATTGATTAGCACTTAAAAAGCGGCGCAATTCAATGATGTTGGACTGCGGTTGGAGTACAAAGTGCTTTAAAAAGTTATTGTGCTGTGCTAGTATTTGCATAATTTTCAACCCACCTAAACCAGCAATAACACCAACAGCAGGATCTAAGTTTAAGTTAGGTAACAGGTCAAACCCGTCACTGGTAATAAAGTGCATGTTAGTGTGATCTTTTAACAACCCTTTAGCAGCACCTAATGCATTAGCACTAATATCACTGTTAACGCCCACTAAACTAGGGTTTTGTTGAACTAGTTCACGTGCTAAATAGCCATGGTCACAACCAATGTCATAAAAGGCTTTGGGTTTTTGCTGTAAGACTAAATTAGCAATTGTGCTAATCCTTCTTTTCATTTCCCCTCATGAAGGAAAGGCTCATGTGGTTATTACGCACTGCTTGACGCAGTTTGCGAATGGCTTTGGTTTCTATTTGCCGAATCTTTTCGCGGGGAATCTTAATCTTTTGACTTACCTCATCGAGTGTTTTGGTTTCGTTGTAAGGTGGCATACCAATGCGCATCCGCACAATTAATTCTTCCTGTTCGGACAAACAGTTGTTTAACAACTCATCAATCTTTTCGTAATTAGAACGGCTTTCGGTAAACTCATCGGGCATTTGGGCATCAGTATCACGCACAAAGTCACCGAACTGCGATTCTTCATCGTGGCCCACTGTTTTATCTAAAGAAACAGGATCTAAGCTTAAACGTTTAATTTCAGCAATCTTTTTAACTGTAAACCCTTCAGCTTGACCACCCATTTTTTCCGCTAACTCTTCCGCGGTGGGTTCGCGTCCTAATTCTTGGTTTAAGGCACGTTCAGCTTTAGCTAAACGGTTAATAGTTTCCACCATGTGTACGGGAATCCGAACCGTACGTGCTTGATCAGCAATAGCACGAGTAATCGCTTGTTTAATCCACCAAGTAGCATAAGTGGAAAACTTATTACCCAATGACCAGTTAAACTTGGAAATGGCCTTTAAAAGTCCTAGGTTACCTTCCTGGATAAGGTCATTAAAATCCAATCCTCTTTCCAAGTGTTTTTTAGCAATGGAGACCACTAACCTAAGGTTAGAGGTTACCAGTTGATTAATGGCATACTTGCGTGATTCTTCATCAGTACTGTTTAAAACTTTGGCAATTCGCTGCTCAGACTCAAAGTCCAACATCTTGGAAGAATCGAGCGAACCTAGAAAGAAACGTACGTTGTCATCAACCTTATCACGATTAGAGATGTTTTTGGTGGTTAATTCTTCAATGTCCTCGTCAATGATCGACAAGTCTTGGTTAGCACGGAACTCATGGATGTGCTCTTCCACATCATGTGGTAGTTCAATTCCCTTGTCACGCAGTTCATCCAAAACAAAGATAATTTCATCATCTGGGAGTTCAAACTTGGACAAAACATTAACAATGTCCTTGTTGGAAAGCGAGTTATTGTCTTTCTTTTTGTTCTTTTTCTCCCACACCTCCACCAACAGGCTAATTACCTTGTTAGTATCATCATTTTCGGTATGACTCTTTAGAGAGCCTTCTAAAATATCAAACAGCTTAAGGTTGTTCTTTTCCTTTAAAGGTGCGTGTTTTGGCTTTCGACCGCGACGCTTCTTTGGTACATCACTCTCCTTGTTTTCTTGAAGCGTTTTTAAAAAGGCTACGTTTTCGTGTTTTTGTTTACGTTGTGCCTTAGTGTCAACGTAAATCATCTTTTTAAACGGACGGTTTTTACCGAGGATACGACTCTTTTTAAGTTCAGCAATGCGCTCTTCGTTAAGTGCTTGTTTTTGATTTTCAGTTTTAGGCTGAGGTTTTTTGAAATTTTTCTTTGGCGATGACATTCAGTCTAGTGCGCACGCGTATACACGCACGCCAAGTGGAATTGTACATTATTTAGTGCTCAACCATGCTTTTAAAATATTTTAAAAGCTCTTTTTGTTTCACAATTAGTTGCCGAAAAAACTGTAAATCTTCCTCATAATCCTCAGGGTCATATGAGAGTGATTTGTTTAAAAAGAACTCCTTAATTTGCGCTAAAAAAGTTGTTGGAAACATTGCTTGTTGTTCCCCTAAAATACTGTTAAACACAGCCCAATCAGGCTCTTGCACCTTAGCTCAATAAAGCCGCGCTTTATCAATAAAAAGGGCAACATCAAACAACGGTGTTTGCAATGAAGCAAACAATTCATCTAAATCAGATTGCTTTAATTCGACTAAAAACTGCTTATCTAACAGTACGTAAGCGAAGATTTCAGCATGAAATACACGTTCCTTGGTCTCCTTTCAGTTGTTTTCGACTAAAAACTCCTTGGTAATGGCAACCGGCTGCGATTCATTTCTAAAACTATTATCAACCACACCAACCACTGATTCGTAAATCGGGGCTCTTTGGGTAAAAGTTGTAGTTTTATTAACTTTTACTTTGAGCTTTTGCTGCTGTAATTGTGTGAACAGTGTTTCCACATCACTGTATTGCAACAATTGCTGTAATTTCTGACACAAAAAGCGGCTAAAGCTAGTGTCAGCTGTTACTTTCTGGTTTTGTGCAATAATGTCGATAACTTTATTGACGGTAATCCGCTCATCAAAGCCCTTGGTTTTAAAGTAAGCCACTAAGTATTCCACGAGGTTTAAAACCCTGTGAGTCTTAGCTTTAACCGCATCACTACCTTCAGCTAAAAACAACTCATCCCAGTCTTTGTAAGCGGCTTGGTTTCAATCGACCACTTCCACAATAATGCCGTGTGCGTTTAACTCTTGTAATAGCTTAAAAGTAGCATCGTGACCCGCTGTATCATTATCGAGTGCCAAGACCACTGTTTCTAGCGCTTTTAGTTGCTGTTGAAAGGCAATAATGTGGCTTTCACTCAAGGCTAAACCCATTAAGCCCACTACATCACCAATCCCAGCGCTAGTAAGGGCAAAGACATCGAAGTAACCCTCAACTAAATACAGCTTTAGTGTGTTTTTATCAATCCGGTGGAAGTTAAATAACAATTCACTCTTCTTAAACCACTGGTGTTCTGCACTGTTTTTGTATTTAATCTTTTCCGTTTTTTGCAGTGCACGCCCCGAAAAACCTACTGGATTACCCTTAAGGTCATGGATCGGAATCATAATCCGGCTCTTAAAGGTTGCATAATGCGTTTGTTGGTTAAAAAATCCTAAAGCTTCTGGGAATTTTTCAGTAGCAAAACAGAGCTCTGCCTTTTCAATTGCCGGGCTAATTCAGGGATAGTTTAAGAGACTGGGTAGAAAATACTGGTCTTCTAAAGTAAACGCTAAACCCAACTGAAAGCGTTCAATTACTTGAGGACTTAACTTTCGTGTTTCAGTTAAGTAAGTCAAACCCTGCTCTTTGGGCTCTAGCTTTAACCGGGTTTGATAGTAATCGACTAGGGCACTGTGTAGATCCCAATAGCGCTTTAACTTTGGGTCAACTTCCTTAAGGTAAAAGTTAACAGCGTATTGTGAGTCAAGATTGAGAATACTAAGTGCCTTATTGAGCGCCGTTTTTCAATCTACCTGATCAATTTTTTGGATAAAAGCAATCCCATCACCGCCCACCTGACACGAGAAACACTTAAAGATGTTCTTTGCAACTGACACCGTCATCGAGGGGTTTTGGTCAGCATGAAAGGGACAGAGTGCCACAAAGTCCTTCCCCTTCTTTTTTAGCTTAATGGCATAGTGCTCCACTATGGGAGCTATTTTGATTTGCTGTTTGAGCTGATCTAGGCTAGTGGGACTGGTCATCGTGTTGGGCAAACCAAGCCTTGAGCTCCTTGATTGGGACACGCACTTGCTTGAGTGAATCCCGTTCTCTGATGGTAACAGCTTGGTCTTCTAAACTTTCAAAGTCAAAGGTAATGGCAAACTTAGTGCCAATGGCATCAGCTTTACGGTACCGTTTGCCAATACTACCGGCGCTTTCAAAGCCAATCCGTCAGTGGGTTTGACTCAGCGCTTCAAATAATGTTTGTGCTGTTTCTTTGAGTTTATTGACTAAGGGCAGCACTACAATTTGTTCTGGACATAAGTGAAAGGGTAAACGTAACACTTCGCGCATTTCCCCTTCCAACTGCTCTTGTTGATAGCTGCTAACAATCAAGGCATAAAACAAGCGCTCAATTCCCACCGAAGGCTCCACTACAGCTGGAATAAAGTGTTCGTTATTCTCTCCATCAAAGAAGTCTAAGGGCTTTTTGGCGTACTTTTGGTGTTGTTCTAAATCAAAGGTACCACGATCAGCTAAACCCCACAATTCCCTCAAGCCATGGGGAAACTGGAATAAAAAATCCACTGTTTTATTGGCATAGTGTGCTAATTCATTCTTGTCATACTCGTATTGTTTAACCAATGCTGGGTTTAATTGCAGTACCTTAAACAAAAAGTGAGCAATTGCTTGTTGTTGGCTTTCAAAAACGCTTAAACTGGCTTGAGGATTGCAGAACCACTCCATTTCAAACTGTTCAAATTCACGAACGCGAAACAAAAAGTTTCCCGGGGTCACTTCATTGCGAAAGCTCTTACCAAACTGGGCTACGCCAAACGGTAAAGGACGCTTTTTGAGTTGTAACAGCTGTTTAAAGTTAATGAAGATGCCCTGAGCTGTTTCCGGACGAAGGTAAACCAGGCGTTTTTCGCTGTTGACAACGCCAATTTCAGTTTGGAACAAGAGGTTAAAATCGCGTACTTCTGACCAATTATTGGCCTTACAGTTAGGACAGTTGACCTGCTTTGGGTTTCACTGGGTGGCGCTTTTAATTTGTTCATCCAAATGATCAACACGAAAACGCAACTTACAGCTCTTACAATCCACAAGAGCATCAGTGAAATTGGCTAAATGGCCACTAGCTTTTCAGATCTGTTCGTTGAGAATAATTGGCGTGTCAATCAACAATACATCCCGCTTATTTTTAATGAAGAAGTTGTACAGTGCCGTTTTAATTTGTTGCTTTAAAACGGCACCCAACGGACCGAAGTCCCAGCTGTTGGCAAGTCCATTGTAAAGCTCACTACTTTGAAAGACAAAGCCATAGCGCTTGAGGTATTGCACATACACTTCTTGGGAATATACTTGAGCCATAGTGTTAATTAATCTTAAAAATTAGCTTTTTCATCCAAACAAGATAATCCCCACGGCAACACTAACATTGAGTGAGTTGAGCTTGGGATTCATCGGAATTTTGACCTTTAAATCAGCGTTTTTTAACAGTAGTGCATTTAAGCCCTTATCTTCATTACCTACCAGTAAAATGCGCTTGTCAAAGTCTACTTGGCGGTAATCTTTTGGCTTTAAGCGCTCATCTAATGTTGTAGCGACCGTTCAAAAGCCGTGTTTTTGCAGGGTAGTAATGGCATAACTGAGGTTAGTAACCTGCACTAAGTTCTGGTAAAAGACAGTTCCCATACTGGTTCTAATAACGGTACTGTTAATGGGCGCCTGGTTATGCTTTTTAAAGATAATCCCATCCACTTCAGCTGCCATACAGGTGCGCAAGATGGCGCCAAAGTTATGCGGATCTTGGATCTCATGGAGCATTACCAGGGTACTGGTGGACTTGCTGTCTGTAATTTGGATTAACTGCTCAATTGTTTTGAGCATTTGGTTAGGGTTGATTACACAAACAAATTCCTGGTGGTTAATGTGGCGAAACTGCTGGTGAAACCACTGGCTGGAGTGCATTTCATACCGCAATCCCCGTTTCTTAATTAAGCCCAACAGCTTTTGGTGCTTATCAGATAAATTGACCTTTTTAATTTGCAGTTGGTTATCTAACGCTTCCAAAAAGGCCTTACTACCAAATAAAAAACTGGTTTCTTGCATCCGCTTCATTACTATAACAAACCTTTTTTAATTAGCTTTTGGCGCACTTCATCAGCCTTGTCTAACTGACCATTTTTGCGCAAACTAGCTCACTTTTTAATTAACTGTACATTGAGCTTAGTGTGGATCGACTTAAAGCCAATGCCCAAGATGTTTAAAGCTCACTCCAAATGGCTTAAATGTTCCTTTAAACCGCTCAAGTTTTGCTTGCTTACATCATGGTTAATTTGTTTGACTAACTTCCAAATGTGGGTAATGGCATTAGCAAAGTTGAGGTTATTGAGAAGTGCCTTAAACACCGGTTCAAACTGTTTGGGCGCGGGAATGGCGGATTGTTCAGAATACACAAACCAAGTGCGGCACACATTGACTGCCTTTTGGATCCTTTTAATATCACTGCAAGCTTGCTCTATTAAAGATTGGCTTAAATCCAACGGGTGGTAATAGTGTTTTTGATAGAACAATCAGCGCAAAATGCGAAAGTCATGGATTGTCAAAAAGTCAACTGCTAAAAGAAAGTTTTGCAGTGACTTGGACATCTTTTGGTTTTCAAACATTAAGTGGCCAATGTGCATCCAATGCTGCGTTAATGGTTTGTCATACAGTGCCATATGCATTGCGTTTTCGTTTTCATGATGGGGAAACTTCAAATCCACCCCACCACCATGAATTGTCAACTGGTCTTTAAAGCTGTAATCAATTAAAAAGGCACACTCCACGTGTCAACCCGGCCGACCCCAACCTCACGGACTGTTTCACTTTACCCCAGCGGTCGTTTGTTTTCACAAAACAAAGTCCAGTTTATTGCGTTTGTCAGTAGCTTCATCCTCAATTGTTTCCACAACACGGTTGGCTAAAATACCATACTGGGGTAATTGGCTTACACTAAAGTACGTGTTGCCCAAAGGCGAAACGTAAGCAAAGCCCTTTTTGACTAACCGGGCAATGTAACCGGGCATTTTGTCAATTTTATCAGTGACTTGGAGGTGTTTAATCGGCAAAATGTTGAGCTTTTTCAACAAGCTTTTGTAAGCGGTGATTTGCTGTTTGCACAACTGCGCTTCACTAATTTCCTGTTGCTGCGCGATCTTGATAATCTTGTCGTCAATATCAGTGATGTTTTGGACAAACTGCACCTTGTACTGCGCTGCTTGCAACACCCTTTGTAGCACATCAAACACAATTAAGGGACGGGTATTGCCTAGGTGCAAATCATTGTAAACAGTCGGTCCACACAGATAAATGTTAATTTGCTTTTGTTCTAATACCACGCTCTGATTGCTCACCGTGTCAATGAGGGTAAATTTAGGTTCAAATTGGTTCATTAATAATGGGAATATTGAGGGCTTTAGCTTGTTCAAGTTTACGAGGTGTAGCCTTAATGCCAGCTAACACAAAATCAACTTTACTAGTGACGTTATTTGTAAAACGACAATCATACTTATTGACGAGCAAGTTTTTAATGGTATCGCGACTAATTGGAAAGCTGCCAGTAATAACAAAACTCTTTTGAAAGTAAATGCTCGCGGTGTCAAAGTTAGTGTTGCTCTTTAAAGCATCCATTTGCACTCCAACTGCTTTAAGTGCAGCTAACAGCTGTAAGTTATTGTCATTGTGAAACCAGTTAAACAACGATTCTGCCACAGTCGGTCCCACATCAGGGAGTTCTAACAGTGTTTCCATACTGGCGGATTGTAAGGCATCGATGTTTTCAAAGTGCTTCGTTAAGCTTTTGGCTAACACTTGCCCCACATGTTTAATGCCAAGCCCAGTTAACAAGCGTGCCATCCCCTTGGTTTTGGACTGTTCGATGCTGCTGATTAGTTTATTGAACAGCACTTCACCAATTTTAAGTTCCAAGGCTAACACCGCGGCTTTGTGCTCCTTTAAGTGGTATAAATCAACGACGCTGCGCACTAAGTTATTTTCATACAGCTTACTAATGGTCTGGATACTTAGCCCCTGGATGTCCATAGCCGTTTTGGACACAAAGTAATTAATCGCTTCAAGGTTACGCTGGTCACAACGTTCATTGGTACAGTACTGATCTACCTCATCCTTATACTGCACTAACGGGGCTTGACATGCCGGACAGTGGGTTTGTTCGGGAATCGGTTGATTCGCTGCTGTTCTTAACGGTAAACACACCTCCAATACCTTAGGGATAATTTCCCCCGCTTTGTAAATTATCACTGTATCGTTTAAACGAATGTCCTTCTCCTTAATGTAGTCAAAGTTATGCAGTGTGGCTGCTGATACTACTGTCCCCTCTAAGTTAACTGGATCTAACTGCGCGGTATAGTTAATCTTGCCAGTGCGTCCCACTGTTACCAAGACACCACGCAGTTTGGTTTGGACAAACTTCGGTTTAAACTTGTACGCCAACGCCCAACGGGGAAACTTACTAGTAAACCCTAACTGGTCGTAAAAGTGAAAGTTATCTAGTTTAACCACTAGGCCATCGACTTGGAAGTTCAACTGATCACGTGTTTGGTCAAACTGCTCGAGGTATTCAATTAATGCTTCGGGTCGATCAAAGCTAGCCACCGCTTTGGACACTGGAAAGTTTCACTGCTTTAACTGTGCTAACACGGCACTTTGACTCCTACAACCCAAAGTTAAGGCGTTGGGAACAAAGTAAAAGAAGGCCTTAAGTTTGCGCTTGGCACTCACCTCACTGTTGAGGTTCCGTAGTGTACCTGCTGCTAAATTGCGGGCATTGGCAAACGGTTTTGCTAAATTCTCGTTAATTTGCGCAAATGTTTTTTTATCGACAAAGATCTCACCGCGGATTTCCACCTGTTTGTGAAAGGGAATCGTTAGGGGAATGCTCTTAATCGTTTTAACATTACTCAAAACATCCTCACCCACAAGTCCATCACCGCGGGTTAAAGCCCGAGTTAACAATCCGTTTTCGTAAGTTAAAGAGATGCTTACACCATCAATTTTGGGTTCCACCACAAAGCGGTTTTGCACTCCAGTTAAGTTGTTAATGTTGGCAATAAAACTAGCAATTGTTGCTGTGTCGAAGGCATTGTCTAGGGAAAGCATTGGATTTTGGTGAGGAAACTTATTAAATGCTTCCACTACAATTCCCCCAACACGTTGGGTTGGTGAATCGGGTTGGATTAACTCAGGATGGGCTTGTTCCAACGCTTGCAGCTGTTTGTATAACTGGTCGTATTCAAAGTCACTGACCAGCGGATCGTCCAAAACATAGTAATGGTAATCATAGCGTTTTAACTGTTCAACTATTGCTCGAATTTGTGCGACCTTTGCCATAAATTAATAAAAAGCCCTGATTAGCTTACAATGCCAACCAGGTGCTTTTAGGAAAATTTAATATGGAGCAGATAACGGGAATCGAACCCGCATCTTAACCTTGGCAAGGTTATGTTCTGCCATTGAACTATATCTGCGCAGCTCATAATTTTAGTATATTAAAGCTAATGGAAACGCAAAACCAAATAGAAACGTTGCGCTATATTTTTAATCAGCTCAACAACCAAGACAAACCACAAATAATTTGGTTTTCCGGTGAAGGTGAGGATGAAAAAATTAACTTTTTAATCCGTTTGGACAATTATTTCCAACCTACCTTTGTCCAAGACCTTACTATTAACTTTCTCCCCGCTTTTGTTAAGCGAAACAAGAAAAATCCACCCAATACTTTAGCTAAGGGTAACTTTGTCAACATAGCTAATAAGTTATTGGCAGTGTTAGCGCGTTCCTTGAGCTGAAAGCAGTTAAACAAACCCCAGCAAAAATGGTTGTTATGGTTACTAGTGCCCTTTTTGCTTTTGCGCCAACTATGGTTAAAAAAGAAGGTAAGCAAGATCTTTCAGTTTGTCAATGAACGTGGCATCTTGAGCTTCATTAAGGAGCAGTGACCGATTTTAACGACACTCGTTACCGTGGGTACTACCTTAGGTACACCCATCTTTTCGATTACCATTTCCCAGCAAAAAGCAATTTTGGAAAACGCTGGTCATGGTGCGTTTGTCTTTTTAGTAATCTTTTCGGTGTTTGCAATTGCGCTGGGTTTAGTCTCATCGTTAATCTTTTTAGTCTCATCGTTATTTTCAATTCGTCAAAAAAAGAGCTTACAACAGCTCCACCAAATTCTCTCTCGGTTAATTAACAAGTACTTCTGTTTTGCTAACAGTGAACAAAATCAAACCGGGCGGTACCAACTGAAAAATACCGGGGTGTGCTTCTTTTATGGCTTTGACTTTGAGGAAAAGGAATACATAACCCAAGCGATGAACCTACTGTTGCTCTTGAAGCAAACGAACTGCTTTGTGTTAGTCGGTTGTAAAGAGAGCAACATGTTGTTAATTAAAAACAAGGTGGAACCCGACATTAACCTTAAACAAAGTAGTCTGTACCTTGATTTAAAGAGTCAAATTAGTCCGTTGGCACAAATTAGTAAGTACAACCTCTTGTTTGAAGAGTTGGCCCTGGATGCTGACATGTTTTACTTAGAGGACTTCTTCGCCTTGTTAAAGACTCCGCGGCAAATTGTCAACTTTTTGTTTCGTATAAAACAAAACTTAAAGGAGTTTCACCAACCCCAAACGTTGTGGTTTGACTATTTGGCCTTGTGAGCCTTAGTAATTGCGACTGACTTTGAATTTAACAATGTCTTGTGGTCATTTAACGACTATTTAAGTTTAACGAACAAGCAAAAGGAAGATTATGCATCAGTTAACCTCACCGCCTTTTTTAACCGTAGTTTAAAGAACCATAAGGACAACAGCCTGCTCTTTAAACCAGAATTGTTCAACACTCACGCTTATATTCCTGAAACTTACACTCAAGTTACCTTAGAAAACATTGATTCAGACAAACGTGCGCAACTAGTGCCATTGAACTGGTTTTCACAGCAAAAGTTTAGCGATTTTATAGAAGAGAAAATTAACTTTTGACAAACTCAGCAGGCGGAAAACAAGGTGTTTTACCTCACTTTGGGCGAGCGGATCTTCTTTTTAGTATTAGTTAACAAGAAGTTCAAACAAATTAAACTAGAAGCAGCGTTGAAGTACCTCAACTAACTTCCCTTGGTGTAATGGATAACACGACTGAGTCCTAATCAGTAAATGGAGGTTCGATTCCTCTAGGGAAGGCCAAATTATCACAGAGACCTAGTTAACTACTAGGTCTTTTGTGTATATAAATTAAATTAAAATGATTAACCGCTAAGGGGGTGTAGTTTAGTGGCAGAACAACAGTCTCCAAAACTGTCTGTGTGGGTTCGATTCCTTCCACCCCCGCCACCTAGTGATGAATTACAGTTACAGCTTTAAAGAATACATCGAACGGTTTGCCAAAAAGGTTAATTCGATTGACAGCGAGTACTTCGAATTTTCCTCTTACATAGAGCGCATGCGCACTGTGTTTGGTTTACTGATTGCACTGATATGCTTTAGTAACGTTCTCTGTTTTCTTTTTATTGCAACTTGGTTTAGCACAAAGGGATTTGGTCAACACTACCGGGCGTTAATATTTACCTTGTTTATTCCCTTTGTCACTTCACTTTTAGCCAACATTATCTTCATTAACCTTAATCGCGCTTTTCGCGAATACTTTAAGATCAGCAGTAAAAGCCGTTCTTTTTTGGTAATCTGTGCCTTCTCCTCATTACCGATTGTGAACATCTGGCTAATGTTGTGGTGAGTAGCGATGATTAAGCGCTTTACTAGTAACTATGCCTTTGCTATCTTTGATAAGTACAACGGTTTGACTAGTGGTGTGTTTATCTTTGACTTTGCTGATAATGTCAATTTTGAGGGTAAACTAGTGAGCTTTGACAATACCAAGGACACCAACCGTGACTTTGTCCACTTTTACAGTGAGGCCAAACTTAAACGCGATAAAATTACGCTACAAACGAATCCAATTCCACACGAAAGGATGTATGTTAACCGCATGTACTATCAGCAAAAGCTCAGTATGGGTGCTAACCAAAACAGCCCTTCAACGGCATTTGCTAACTTAAAGCGGTATGTGGAACATAAACAGCAAAAAATTATCAAGATTAAGCAGTTTATCTTGACCTAAAAAAGCAATAAATATATAATCTAAGCCGTTGCTTTGTAAAACCAATGAAAAAAGATTTTCATTTTCCAAGCCAATCCGTTAGCTTCAAGTGTGCTTCTTGCAGCAATAGTTTCACCATTGAATCAACCCTAAAACAAAAAGAAATTACGATTGATATTTGTGGTAAATGTCACCCGTTTTACATTGGTGAATTAACCAAACAAACAGTCCACGGTCGTGCGGAAAAGCTTTCTGGAAAGTTCAATGCTGGCAAAGCCTTTTTAGAGAATAAAACTCCTAAAAAAGCGAAAGGCAAAACAGAAGAATATACCAAACACCGTTCTTTAAACGAACTGTAGGTGGAGTTTGACAAGCAGTTCTTTTCTAGTGTTGAAAAGATTGTTGAACTTGCTGAACAACTCGAAAAGGACTTAAATAAACCCGATTTGACCTTTGAACAGATCAAAGCGATCAACAAGGAACTGAAACATAAACAACCCTTAGTTGTTAAATTCAAAGAATTTAAACGGCTCATAGATCAAGCTTTGGAAGCGGAGGCCATTTTGGAAAACAACGAGCTCAAGGAGCTACATGATGAGGCCAAAAAAGAGCTGGAACGGGTGCGCAGTGTTGTCCCTGAATACGAAGAAGCGTTAAAACTGTTGTTATTACCAATTGATGAGAATAACCAGAAAAACGTGATTGTAGAGCTTCGTCCCGCTGCTGGTGGGGATGAATCGTGCATCTTTTTAGCTGATTTATTCAATATGTACCGCAACTTTTGTACTAACAAGGGTTGAAAGTTGCAAATTAACGAAATGATCCCATCCAGTGTCGGGTTAAACTTCGTTTCCTTTGAAGTTAATGGTGTCGATGTGTTTGCCAAGCTCAAGTTTGAATCTGGCGTACACCGGGTACAACGCGTACCCGCTACTGAATCCAAGGGGAGAGTACACACTTCAACCGTTACGGTTGCTGTTTTACCACAACTAGAAGCGGTTGAAGTTCATATTAACCCAGCTGATCTTAGAGTAGATACCTACCGTGCTTCGGGTGCTGGGGGTCAACACGTTAACCGGACTGAAAGTGCAGTGCGGATTACCCATTTACCAACTGGCATAGTAGTTTCGTGTCAAGAGGGTAAATCACAATTTACTAACCGTGATACGGCAATGAAGATGCTACGTGCTAAACTGTGGGAAAAAGCGCAGAACGAACAGTTATCAACCCAAGCAGGTTTGCGTAAAAGTCAGGTTGGGTCAGGTGATCGTGCCGAAAAGATCCGCACATATAACTATCCCCAAAACCGGGTCACAGACCACCGCATTAAACTTACCGTCAATAAGCTTAACACGATTATTTTAGGTGACCTTGATGAAATTATTGAAGCGTTGCAGGCAGATGAAAAGAAACAACAACTAGAGAACTTTTTCAGTTAATGAACTTGTATGAACTTTTCTTGAACCAAAAGCTGCTGTATGGCACCGATCCGCACTTTAACGGTGTTTTTCTCACCCTATTAGAAAAGTTTGGTCTGCACTTTAAGGATTTAACAGCACTGTGAAAGCACGCTAAAACAATTACTGACTTTGACGAACAGGGAATTGTTAATGCTTTAAAAGCTTACTTTGTCGATCAACTACCCTTACCCTATATTACCGGGAGTGTTAAGCTAGGTTCATTAACTTTTAAAACTCAACCTGGCGTCTTTATTCCTCGTGCCGACAGTTTAGCGCTTTTAAAGGTAGTCAAAGCACAAAACCTTAAAACCGCGGTTGACCTTTGCTGTGGAAGCGGTACGTTAGCAATTGCGCTAAAAAAGCGTTTTCCTCACTTAAATGTTTATGGTAGTGACCTCAACCCCCAAGCATTGCAGTTAGCGGCGCAAAATGCCCGTTTGAATATGGTTGAAGTGCAGTGAATTGAAGCTGATTTTTTAGCTGCTCTAGCACAGGTAAACACACCTATTGATCTCATTATTACCAATCCACCCTATTTAAACGAAAGTCAGTTAGATCAAACTTTAAACCACGAACCGCGCAACAGTTTAGTGGCTGATGGTAACGGAATTTTGTTTTACCAAAAGCTGTATAACTTTTTATTGGGTAACCGCCAAGTTAAACAAGTGATACTGGAGTGCTCACCAACCCAGAAAAAAGAGTTTTTAGCCTTATTTAGCATTTTTAAAACTAGTGAAATTTACACAAGCCACAAGCAATTCATCGGTTTGAGTATTGACAACACTAAACTACCAGTCTTAAAAATTGCGCAAACCAAGCAAATTAAAGCACTGCTAGACAAGGGGATGACGGCCATTATCCCCACCGACACGCAAATAGGATTAATGAGTTATTGCCAACAAGATTTAGACCACATTAAACAACGCGATCCCAACAAGCACTACGTCCAGTTTTTAGCACCTAGTCAAATTAACCAGTTACCCAAACAATTACAAAAGCTAGCCAAACTGTTTTGACCAGGTGCTTACACCTTTATTGTAGATGGACAGAGCTATCGTCTACCCAACAGCCCACAGCTGTTAAAGCTCTTAAAAACGGTTGGTTTAATTTACTGTACCAGTGCTAATCAAGCTAAACAAAAACCATTTGGCAAGTTAAGCGCTTACCAGAACGATCCTTACTGAGTGCAGCAGAACTGTTTTATTGTGCAAAACAGCTTTAAAAGTAATAACGAACCGTCCTTAATTTACAACTTGGATACGAAGCAAATTGTTCGTGGTAGTTCAACCCAATTACAACGTTTCCAAGCGTTACTAGCAAAACACAAATTAAGACACTAAAAGTTTCAAATTTGACTGCTAAAAAGCACTATTAACGGATTATTGGAGCTATCCAACTTATTTTTAACTTTTAAAAATAAAGAATTTGCACCATTGCTGTATAATTTCCCATCTTTTTGGTGTTTTGTAAGATGAAATTTAAGTATGGTGCTACCCTTTTTAGTGGCTTTTTAGGGCTTTCAGCGATCCTAGCTGCTTGTGGTGCAAAGGGTAAATTTGACCAAGTTGATGATGGCAAAATTGTGTTAGCCTCTTCATTAACTTCTAAAAATGCCGCTAATGCTCTACAAGCTGTGGTGGAAAAATACAACCAGGTTAAGGGTGGCAATGATTATCCTATTGAAATTACCCAAATTACCGGTGGTTATGACGGTGGACGTGGTAACTTACAAACCAAACTTAGCGTTAAAGACAAAACGACCTTTTACAACCTCATCTAAACTATCCCGACTTAGTTTCCACCCTAGGAAGTGTTGGGATGGAACTGCCACTAGACAACGTTGATCTCTCCAAGTTATCACCTAACTTTTTGAAGTTCAACGAACGCATTAGCGGTGTAGCCAAAAAGGCCAATTACGCCATTCCCGTATCACTTTCAACTGAAGTTTTAGCTTTAAATGGACCGGTATTACACTATATCTTGAACAGCGCGAAGGGTGGTAATGAAACAGCTGTAACACAAAGAACTGCTAGCACAAACCAACAAAATACACTTCAAAATTCGTTAAAAATTAACGAAAGCGACCCCATAACCAAAGAGCTTTGAGAAAAAATTCAAAAGGCTGCTGAAACTAATGGAAAGGCTAAAGAACAAGGTAAAAGTAAGAAACAAGGCACAGAGAAAACTACCACTCTAACATTACTTAAAACTGAATCTAGTCAACAATCTAACACCACTAAAGATGGTAAAGCTACAAGCGATGAAGCAATTAAGAAAACTTGAGGTGATTACCAAAAGGTGGAGAATGGCCTTAAGGGTTATACCTTTAAAGCTAGTGTCTTTGAAAACTGGCATGAACTGCTCGATTTTTCCGCAAGGATAGCTAGATCATTTACTAATATTCACACCAAAGAAACCAAAAAGGGAACGGACATTCAAGGTGTACTTGGGATTGACAGTACACCTAACGCCTTATTTACTTCAGTATTTGCTGCAGGTGGTGGTAACTATGACAACTTCTTTTATAAGGTGCAAGATGGTCGGGCTGACTTTAGTAACTTTAAAAATAAGGAAACCTCTTACCAAAACTTACAAAAAGTATTTAGCGATTATAAGGAGTTAATTGATAAAAACGGTTTGTTTGTCAATAAGGGTGGCTCTTACTCTTCTAACTTCCAGAAGTTCCACCAATTAGCTTACAGTATTTCTTCCACTTCAGGTTTCTTTTATTCGTTTGCTGGTAACAGTGCTAAACGCTTAAAGTTTGATGACGGTAACTTTATTGAGTTTCCGCGCTTCACTCAACCAGTTAAAGCGCCTAATAAAACAACTCAAAGTGGTGGAGAAAATAACAATAAAAGTGAAAATAATCTTCTTGGAACTTTTGAAATAGAAGACAAAAGCAAAAAGCGCGAAAGCGTTGAATCTAGTGAAAAATCAAGTTCTGGGACTCAATCCAAAAGCAAGAAAGGTAAAAGTATGAAAACCATTTACCTTTATAAAGCACAAATTCCAGAAGGAAAAAACAAAGGTGATAATGCCATCTTAATTACTAACCAAACTGTTATTGATAAACTAGAAAAAGCTGCTAAAGAAGACGGTAAAAGTGGAACTCAAGTTTCAACTATTGCAAGTAAATCAACAACTAAAGAGAACCAAGAAAACAAAATAATTGGTTACACATCGACTGATAACCTTCGTGAAGACAATAAGAACATTTTCGATATCGATGATCTTAACGATGAAAAATATGACCGTAAGATTATTGTTAGTGCTACTGAAGAAGTATTGGAACAATCTAACACTTTACAGAGTGAAGAAGCAGTTGTTCTACCGGCACCTGCTAAATATAAAGCTAGTGATTCCACAAAAATAGCAATCACCCAAGAACCTAACCTAATTGGTATCCATGCTAACGAAAAGGAAAATGAGGAAACACGTAAGTTTGTTAATTGGTTTTTAAACAGTGAGCAAACTTGGGAATCCAAGAAATACAGTAAAAATAAGCAAAACTCCAACGAAAAGAGAACTCCTGCACAATTCTTTGCTGATTCCGCGTCTTACATTCTACCCTTAAAGAAAACCTTCGATAATGCCAACAAAAAAGAAGATCAAAGTAAGGCCAATAACAAGACTAATAGCCAAAGAACTAACACCTATATCGAAAAGGCATTAGAAATTTTTAAAGGAATTAGCCAAAACCAAATAACAAGTTACAGTGATCCAAGCGACTTTAGAAGTGGTCGCTTCCGCGATGGTATTGGTTCTAACTTTAATGCAGTAGTAAATTCCAAAGTGGATTTCAATAAATTTATTAATAACTTTATTGCTAGTCTAGGTTCTGACATCTAATAAAGCTCTTGTTGAAACAAATTAAACTGTTACTGTCGTGGAGATTAAAACTTACAAAATCAAAGATATTTGCGAGATTTCTAGGGGCAAAGCAATTACCAAAGAATACATAAGAGCTAACCCTGGTGAATATCCTGTTTATTCTGGTTCAACATTAAATGATGGTGAAATAGGTCGCATAGATGAATGCGAATTTGATGGTGAATATGTAACTTGAACAATTGATGGTTATGCTGGCATAGTTTTTTACCGCAATGAGAGGTTTAATGCCTCTCAACATTGTGGTGTCTTAAAGGTAAAAAGCAATGAAATTTGCCCTAAATTTTTGGCATATGCATTGGGGATGGAAGCGCCTAAACATGTTAATAATGCTTGTGTTATTCCTAATCTAACTTTAAAAAAGATGAGAGAAATAGAACTAGATTTTCCAAGCAAGAAAATTCAAGAGAAAATTGCTACTATCTTAGATACTTTTACCGAGCTAAGCGCCGAGCTAAGAGAGCGAAAGAAGCAGTATGCCTTCTATCGCGATTATCTGCTCAACCAAGAAAATATCCGAAAAATTTACGGAGCAAATATACCTTTCGAAACTTTTCAAGTAAAGGATATTTGTGAAATTAGAAGAGGTCGAGCGATCACTAAAGCGTATATTAGGAATAATCCTGGAGAAAATCCAGTTTATTCCGCAGCTACCACAAATGATGGTGAGTTAGGACGTATTAAAGATTGCGATTTTGATGGTGAATACATCACTTGAACTACAAATGGTTATGCTGGAGTAGTTTTTTATCGTAATGGCAAGTTTAATGCATCACAAGATTGCGGTGTTTTAAAGGTTAAAAATAAAAAAATCTGCACCAAATTCCTATCCTTTTTATTAAAGATAGAAGCACCAAAATTCGTTCATAATCTAGCTTCAAGACCAAAACTAAGCCAAAAAGTAATGGCTGAAATCGAACTCTCTTTTCCACCTCTAGAAATTCAAGAAAAGATTGCTGATATTCTCTTTGCCTTTGAAAAACTTTGCAATGATTTAGTAGAAGGTATTCCTGCTGAAGTCGAAATGCGTAAAAAGCAATTGGATTACTATCAGAACTTCTTATTTAATTGAGTGCAAGAACAAAAAACTCAATTAGAGCAAATTATGTAATGCTTAATAAATAGTTTCCAACACCTACCTCCTCCAAGACCACAACACCCTCACCCCCTACACGCCCTTTACCACACCGCTCAATGGGGGGGCTGGATGTTGTGCGGGCCGCCCATTTACACCCCTCATACGAACTCGTGGACTGAAAGCGGGTGGGACAGAACAAGTTAGTGGCGCTGGTCCGCTCAGCGTTGGTCAGGGTGAAATTCCAGGACACAACGAGTTCGGACTCTAATAACCAAGATACGAGTCAAAATGCCTTGAGTTTTGATACCCAAGAATCACAGAAGGCACTTAATGGCTCGCAGAGTGGATCTTCTGACACTTCCGGGTCTAACTCCCAAGACTTCGCCAGCTACATCCTCATCTTCCAAGCCGCGCCCAGGGCCACGTGGGTGTTTGAACGGAAGATTAAGTTGGCGTTGCCCTACGTTAAGCAGGAAAGTCAGGGTTCCGACGATCAAGGTTCCAATGGTAAGGGCTCCCTCTACACCACCCTCCAAGACCTCCTCGTCGAACAACCCGTGACCCCTTATACGCCCAATGCGGGGTTAGCCCGGGTGAATGGGGTTGCTCAGGATACGGTTCATTTTGGTTCGGGTCAAGAATCGAGTTGGAATTCCCAACGTTCCCAAAAAGGCCTTAAAACAACCCCGCTCCCAATGCCGTCACCGGCTTTAAGCTCGATAAGGGCCGCGCGTACCGGAAGCTGAATGAAAGTTGACCGGTGTATGAACCCCTGGATTCGACCAAGGAGGGGAAGGGGAAGGATGAGAGCTCTTGGAAAAATTCGGAAAAAACAACGGCGGAAAATGATGCCCCGTTGGTGGGGATGGTTGGAAGTGGTGCGGCTGGAAGTGCTTCTAGTTTACAAGGCAATGGCTCGAACAGTTCGGGGTTAAAATCGCTCTTGAGATCAGCACCTGTCAGTGTTCCACCAAGCAGTACAAGTAATCAAACTTTAAGCTTATCTAACCCCGCTCCTGTAGGCCCACAAGCGGTTGTAAGCCAACCCGCGGGGGGTGCTACGGCAGCAGTGTCCGTCAATCGCACAGCGAGTGACACCGCCACCTTTAGCAAGTACCTCAACACCGCCCAGGCCTTGCACCAGATGGGGGTGATTGTTCCGGGGTTGGAAAAATGAGGTGGTAACAACGGTACGGGTGTAGTGGCTAGCCGACGGGATGCTACTTCCACTAACCTGCCCCATGCGGCAGGTGCTTCCCAAACGGGTTTGGGAACTGGTTCGCCCCGCGAACCAGCTTTAACCGCAACGTCACAGCGTGCCGTCACGGTGGTTGCTGGCCCCCTTCGTGCGGGCAATAGCAGTGAAACTGATGCCCTACCGAATGTCATCACCCAGCTCTATCATACTTCAACCGCCCAACTCGCTTACTTAAATGGCCAGATCGTTGTGATGAGTTCCGACCGGGTACCGAGTCTTTGGTATTGAGTTGTCGGGGAGGACCAGGAATCGGGCAAAGCGACCTGATGAGCGAAAACCGAGCTCAACTGGGGCACCGACAAGCAGAAGCAGTTTGTCGAAAACCAGTTGGGGTTTAAAGATGACTCAAATTCGGATTCCAAAAATTCGAATTTGAAGACCCAAGGCCTCACCCAACCCGCCTACCTCATCGCCGGTCTTGACGTTGTGGCCGACCACCTCGTCTTTGCGGCATTTAAAGCGGGCGCGGTGGGGTATGATATGACGACTGATTCGAACGCTTCGACCTACAACCAAGCACTCGTCTGGTCGACCACGGCCGGGTTGGACAGTGATGGGGGGACAAGGCTTTGGTAGAAAACACGGCCGGGCTCAACGGCCCGATTAATGGTTTGTTTACCCTGCTCGACACCTTTGCGTATGTGACCCCCGTGAGTGGGATGAAAGGGGGGAGTCAGAATAATGAAGAAGTGCAAACGACTTACCCGGTCAAGTCCGACCAAAAGGCCACCGCCAAAATTGCCTCCTTAATTAATGCCAGCCCACTCAACAGTTATGGGGATGATGGCGATAAAAAAGCCATCTACCCAGAGGTAAGAGGCAGACAGCTCCAAAATATTTTTGACGATTGGAAAACTAACAAAAGCTGTTAGTTTGATGTAGAGGCAAAACTATGAATTTACTAAATTTATTTAAATCAGCGGGAGCAGGCTCGTTTTTTCAACAAAAGTCACTCTATCTGCTCCCACTAAATAAATTGACAGAGTTTTATAAAAAAGCCAAAGAGTTTGAATATTTTTGTAATTTGGGTTTTTAATACCTTCTAACTATTAATAGGAACTTTTCTTATTAATAAGAACTTTTTTGAAAAAAGTTTTGAAGTTATTTTGGTGTCTTTACTAACTTTAAGCAATTTAGTTAGTGTAAGCACAAAAACCGTTATGTGTAATGTTTTTATTAATTTAGCAAAACAGCTGAACTTAGTTGGCAGCACTGCTGCAAGTAGTAGTTTTCTCAACCTCTTTCAGAAAGGAGTCGCTTATTAATGAAAGAGAAAATTCCGTTTTATAACGAAAAAGAATTCCATTACATGATGAAAAAGACCAAAAAAGGCACTTTTTCGGGATGATACATCATTAATCCAGAAAACAACAGTGTGGAATTCTCTGGCAGTTTCAACAGACAATTTAAACTTAACAAACCGGTTATACCAGTGAACACAGAATACGTAACGCGTAAAGAGTTCAACGAGTACAAAGACTCAAACGACCAACGACTTACAAAGATTGAAAATAAGGTCGATAAGCTTGAAATTAAAGTTGATAAGCTAGAGAAGAAAGTCGATAAGCTGGAAGTTAAGGTTGATAAACTAGTCGAAACTGTTAATGCTCAAGGAGAAGATCTTAACAACTTCAAAGTTGAAGTGAGAGGAACACTTCAAAGCCAAGGCGAAACCCTGCAATTAATTTTGCAAACGCTACAAGGCATGAGTAAGCGGCTTGATTCTGTTGAAGGACGTTTAGACTCTATGGATGGTCGTCTCGATTCAATGGAAACTCGTTTGGACAAAATCGATCCGCTAAAATAGTCTTAGGCTAATAACAAAAGTGCCAGCAATTAATCTATAAAGGTTAGTCGCTGGTATTTTCATTTATTTGAAATAAATAAAAAAGCCATCCTATCAAGGAGAAAGACAGACAGCTAATGAATTGAAATCAATTACTTAATTAAATATTCCAAGTAAATGAAGAGATTAATATCTGATGAATAGCCTTCTGTATTATTAGCAAAGGTACTTAAAAATTTGTTCACACTTTTTTCACTAACATCATCTTTGCCTTTTAATGAAAAGCTAAGTGAAGCGCTAAACTCAATTCACTCTAAATTTCTTGTAATTTCTTTTCCATTAACTTTAATTTTTTCTTCTTTAGTAAGAAATTTTTCTGTAGTAAATTGTTTTCCTGGATCAGAATTAGATTTAATTTTAAGGAAAAGATCAAAATAATGCTTGTCACCTTTCTTAGCAGTGAAATCACCTGAAGAATAATCTCCAAACCAATTAAAGGTATAGCTCAACTTAGCTTCAATTGACAAATCCTTAAAACTTTGGTATGTCATCTGGAAATATTCAATCTTTCGCTCCATCGTAAAAGGATCCTTGCTTGCGGGATAAATTACATCGTCAAAATCAAAATCTGGAATACCTAATTCGCCCTTTTCCGCACTTTGTTTTCAGGCGTCTAACAACAATTTTGTTGTATCTTTCGGATTAACTTTGTAAGACTTCTTTAGAGAAGTTATCATGTTCTTTTTGCCTTCTGTAGTTTTAATTGCTTTATACACATCAATGTCTTTACCGGTGGCTTCTTTAATAAGATTTCTAAGATCTGCTTGGATACCAGTACAGGCGCTTAAAACTGTTCCACATAACAAAAATAAAGAACCAAAGGAAACCTTTGACAAAAACTTCTTTTTGCGCATCAACAGTAAAAATAACTCTTTAATTTTGCTTTTTTTGTAAAAAGCCTTTTTGGGATAAGAACTTGAATTGGAATTTCGGGTTGAACATTAATTAAAACACTGAAATTTCCAATCATTTATTAACAACTATCTACTGATAAAGGTTTTGATCTGAGTAGTTCTAATAATTGCTAATTGTTGGCATTTTTATTCATTTAATGCTTTCTAAAAACTTTTTGCAATTTTGTTGGCACTTCTCAATAAGTCGGTCAATTTATTTAGTGGAAGAGCAAATGCAAATCTTTAATTTTTTGAATTGATCAGCAAAGCGTCTGATCCCTGTTGGCAACCGAGTTGCAAGTGTTAGTTATTTTTTAAAAAATCAAAAGAAAAAAGAAAGGAACATTTAAACATGAAAAAAGTTAAAGCATTAAGGAATCTTTTTCTAGAAAAAGCCAATGAAAAACTTTAATACCCCAAAATTGCTTCAATGGTAGCTTACTTTGATCCAAAGGAAGACGGTACTTGTGAATTCATTATGAAAATTAAAACTGTAAATGAAGTTGCTGACACTGAGATAACAAATAAGGAAAAGCCTTTAGGCACTTGAAAGAGTTAGTTAACTTTTTGGTCTAAAAAGACATAAATTTACCAACGGTAAGTGTTAAAACTTGCTGTTGGTATTTTTATTTCCCCCACGCTCACCAGGTTTAGTACCGGGTTCAACCTCGCGGGGTCGGTGCTCGACCAGGTGTTGGATTATGTGCCCTGGATTGGGAATGGGCACAGGTATGGGAATAACCACCGGGGCGTGGATGATATAACCGCGCCTAAAACCGGCGCGGGGTCGTCCAGCGGAACTAGTACGAACACAAGTGGTTCGCGTTCCTTTCTCCCGACGTTCTCGAATGTCGGCGTTGGCCTCAAAGCGAATGTCCAGGGCACCCTGGGTGGCAGTCAGACGACGACTACGGGGAAAGATATTCCCAAATGACCCACCCTCGACCCAGCCAACCTCCAGCTCTGAACGGGGGCGGGGTGAAGGAATGATAAGGCTTCAAACAAACAAAGTGACGAAAACCACACCACCTTTAAAAGCGCTACGGGCAGTGGCCAGCAGGGTGGTTCTACAACGGGTGGTTCAGCGGGCAATCCCGACTCGTTAAAGCAGGATAAGATTAGTAAATCAGGTCAGAACTTAACCACGCAGGACGGCGCGCCCCAGTCTAATTCGACGACGGAATCCGCGTCGAATTATGATCACCTCCCCCCCAACCTCACCCCCACATCCGATTGACCGAACGCGCTGTCATTCACCAACAAGAACAACGCGCAGCGCGCCCAGCTCTTCCTCCGCGGCTTGTTGGGCAGCATCCCGGTGTTGGTGAATCGAAGTGGGTCAGATGATTCCAACAAATTCCAAGCCACCGACCAAAAATGGTCTTACACCGACTTAAAGTCGGACCAAACCAAGCTCAACCTCCCCGCTTATGGTGAAGTGAATGGGTTGTTGAATCCGGCGTTGGTGGAAACCTATTTTGGGACGACGCGAGCGGGTGGTTCGGGGTCCAACACGACCAGTTCACCCGGTATCGGTTTTAAAATTCCCGAACAAAATAATGATTCGAAGGCTGTGCTGATCACCCCCGGGTTGGCTTGAACGCCGCAAGACGTTGGTAACCTCGTTGTCAGTGGTACCAGCTTGAGCTTCCAGTTGGGCGGGTGGCTGGTCACCTTCACGGACTTTGTCAAACCCCGCGCGGGTTACCTCGGGCTCCAGTTAACGGGCTTGGATGCAAGTGATGCGACGCAGCGCGCCCTCATTTGGGCCAAGCGGCCCTGAGCGGCCTTTCGTGGCAGTTGGGTCAACCGCTTGGGCCGGGTCGAGAGTGTGTGGGATTTAAAGGGGGTGTGACAAGATCAAGCTCAGGCGGCCGCGCAAGCAGCTACCACCGCCGCCGCAACAGGGGACGCCTTACCGGAGCACCCCAATGCCCTCGCGTACCAAATTAGCTCCACCGACAAGGATTCGTACAAGGCTTCCACTCAAAGCTCCGGTCAAACCAATTCCCAAAACACCTCCCCCTACCTCCATTTGATTAAACCTAAGAAAGTCGAAAACACGACCCAACTCGACCAGGGCTTAAAAACCTGTTGGACCCCAACCAGGTTCGCACCAAGCTGCGCCAAAGCTTTGGTACAGACCATTCCACCCAAGCCAAACCCCAATCCCTCAAAACAACCACACCGGTGTTTGGGACGAATAGTGGTAACATTGGCAGTGTGCTTAGTGGTGGGGGTGCTGGAGGAGCAGACAGCACCAATTCGGTGGACCTCTCCCCCGTTGAACGGGTGAGTGGGTGGCTTGTGGGGCAATTACCCAGTGGGGGTGGGGGGAATAGTAGTGAGGATATTAAAAGTGTGCAAGACACTCCTATTTATATCTATATTCATTAGTATATTCTTTTTAAATTGTTCCCTCACTTTATTCATATGAACCACCGCCTCCCTCGCGACGGGACTCACCGTAGTGGGACACTTCACAAGTACCACCACGACGCTCAAGCGCCAGCAATTTAGCTACACCCGCCCTGACGAGGTCGCGCTGCGCCACACCAATGCCATCAACCCGCGCTTAACCCCGTGAACGTATCGTAACACGAGCTTTTCGTCCCTCCCCCTCACGGGTGAAAACCCCGGGGCGTGGGCCTTAGTGCGCGACAACACCGCCAAGGGCATCACTGCCGGCAGTGGCAGTCAACAAACCACGTATGATCCCACCCGAACCGAAGCGGCTTTGACCACCGCCACCACCTTTGTGTTACGCCGATACGACCTCGCCGGGCGTTGTACGACCTCGACTTTTCGAAGTTAAACCCGCAAACGCCCACGCGCGACCAAACCGGGCAGATCACCTTTAACCCCTTTGGCGGCTTTGGTTTGAGTGGGGCTGCACCCCAACAGTGAAACGAGGTCAAAAACAAGGACCCCGTCGAGGTGGCGCAAGACCCCTCCAATCCCTACCGGTTTGCCGTTTTACTCGTGCCGCGCAGCGTGGTGTACTATGAGCAGTTGCAAAGGGGGTTGGGCTTACCACAGCAGCGAACCGAGAATGGTCAAAATACTTCCACCACCGGGGCAATGTTTGGCTTGAAGGTGAAGAACGCCGAGGCGGACACCGCGAAGAGCAATGAAAAACTCCAGGGCGCTGAGGCCACTGGTTCTTCAACCACATCTGGATCTGGCCAATCCACCCAACGTGGGGTTCGTCAGGGGACACCAAAGTCAAGGCGTTGCAGGTGGCGGTGAAAAAGAAATCGGACTCGGAGGACAATGGTCAGATCGAACTTGAAACCAACAACCTCGCCAACGCCCCGATTAAACGGGGCTCCAATAACAACCAGCAAGTCCAACTCAAGGCGGACGATTTTGGTACTGCCCCTTCCAGTTCGGGATCAGGCACCCAAGATGGCACCCCCACCCCCTGAACGCCGTGGTTAACGACTGAGCAAATTCACAACGACCCCGCCAAATTCGCCGCCTCGATCCTCATTCTGTACGATGCGCCTTATGCGCGCAACCGTACCGTCATTGACCGCGTTGATCACTTGGATCCCAAGGTGATGGTGGATAATTACTGTTAACAATTTTTTTAGAAAAAGGCAGTTTTTGAAGTTTTTAATTTGTAAAATTTCATTTTTTAAAAATGCCAAATCCTGTTAGATTTGTTTACCGTGTTGATTTGAGAAGCCCTGAAGAAATTTTTGAACATGGCTTTTCAACTTTAGGTGATGTGAGAAATTTCTTTGAACACATTCTCTCCACTAATTTTGGTAGAAGCTATTTTATTTCCACTTCAGAAACACCCACAGCAGCTATTCGCTTCTTTGGTAGCTGGTTACGGGAATATGTACCAGAGCACCCCAGAAGGGCTTACTTATATGAAATTCGTGCCGACCAACACTTTTACAATGCCCGCGCCACTGGGGAGAACTTGTTAGATTTAATGCGTCAAAGACAAGTAGTATTTGACTCTGGTGATCGAGAAATGGCACAAATGGGAATTAGAGCTTTACGCACTTCCTTTGCGTATCAACGTGAATGGTTTACCGATGGTCCAATTGCAGCAGCTAATGTCCGTAGTGCTTGACTAGTAGATGCTGTTCCCGTTGAACCTGGTCATGCTCACCACCCGGCTGGTCGTGTTGTAGAGACTACTAGAATTAATGAACCGGAAATGCACAACCCTCATTATCAAGAGCTGCAAACCCAAGCCAATGATCAACCATGATTGCCAACACCAGGAATAGCTACTCCTGTACATTTATCAATTCCCCAAGCAGCTTCCGTTGCTGATGTTTCGGAAGGTACTTCCGCTTCGCTATCGTTTGCGTGCCCTGATTGAAGTCCACCTTCTAGTAATGGTGAAAATCCGCTAGACAAATGCATTGCGGAAAAGATTGATAACTATAACCTACAATCCTTACCACAGTACGCTAGCAGTGTAAAGGAACTGGAAGATACACCAGTATACCTAAGGGGAATTAAAACGCAAAAAACCTTTATGTTACAAGCAGATCCGCAAAATAACAATGTCTTTTTGGTCGAAGTAAACCCCAAACAAAAGTCCAGCTTTCCCCAAACCATCTTCTTTTGGGATGTTTATCAACGAATTTGTCTCAAGGATTTAACTGGTGCACAAATCAGTCTTTCGCTTACTGCCTTTACTACTCAGTATGCTGGTCAGCTCAAAGTGCACCTTAGTGTTAGCGCGGTTAATGCCGTGAACCAAAAGTGAAAAATGACACCGCAAGACATTGCAATAACTCAGTTTCGGGTCTCCTCTGAACTGTTAGGTCAAACTGAAAATGGCTTGTTCTGAAATACCAAGAGTGGTGGTTCACAACACGATTTGTATGTATGTCCTTTGAAAAATCCACCTAGTGATTTGGAAGAATTACAAATAATTGTTGATGAATGTACTACCCATGCGCAGTTTGTTACTATGCGTGCAGCTAGCACCTTCTTTGTTGATGTTCAGCTAGGCTGGTATTGAAGGGGTTATTACTATACCCCACAATTAAGTGGTTGATCTTATCAGATGAAAACACCAGATGGACAGATATTCTATGATCTAAAAACTTCGAAAATCTTCTTTGTCCAGGACAACCAAAACGTGTTCTTTCTCCATAATAAACTCAACAAACAAACTGGTTACAGCTGGGATTGAGTAGAATGGCTAAAACATGACATGAATGAGGACAAAGACGAAAACTTTAAATGGTACTTTTCGCGTGATGACCTTACCATTCCTTCCGTTGAAGGGCTTAACTTCCGCCACATTCGCTGTTACGCTGACAACCAGCAGTTAAAGGTGATCATAAGCGGTTCACGTTGGGGCGGTTGGTACTCCACTTACGATAAAGTTGAAAGTAATGTCGAAGATAAGATTTTGGTCAAAGATGGTTTTGATCGCTTTTAGCGATTAAGCTTTAACGTCACTGTTTTGCTCTAATGTTAGAAGCAAAGATCTTGTCGACAAACTCGTTGCGCTTGCGGTAACGACGTACTACTAAATAAGTTACAGCACCAGCAACTACTACACTAGCTATAGCAGCCACAGTTAAACCAGCTACCAACGGATCTACGTGGGTTAAGAAGTTGAAACTGCCACCATTTGCATTCAATTCAACGGTAAAGCCATCTAGTGATCTTTCACCTTGAACTAGTTGTTGGCTAGACAATGAGCGTGGTTTGAAGAGGTCAAATGAACCATTAGCACCAACTTGGTTTTCGTACTTAACTTGTAAGAACTCCTTCAATTGTCTTTGGAAGGTACTTTGAATTGGTGCATAAGCATCATTAATAAATGAGCTGTCGGTAAACAATGCCCCTACAATCATGTTGTACTCATACGGATTGAGTCCAGAGAAATCAAACACAAGTGTTTTCTGCTCCTTATTATTTCCCACACTTCTATTTTCTATTTTGTAAGGAATATCAACCGTAGTGCTTTGGTTTGGGTTGAATGCATTCTTAGTTAAGGCAATTCTTAGATTACCACCTTCAGCATTATTGTCAGTTTGGGCACCGCCATCACTTACCATAACTGTGTAAGATTGTGCTTCCTGTGCCAATTTGTTGACATCATTTGGGTCATTCAAACCTAAAGAGCCATTTTGTTCAAGCGAGAAGTCGGCTTTTAAGTCTAGCTTGATGCCATCCTTTTCAACATCAAACTTAATTTCGTCCTTTAAGAAAACCTTCTTGTTAGTTAGTCTCTCTACATAACCAACCACCGCACCACTAGCAGCTTGACTTAAAACACCACGTTTAGAACTCTCGTTGGACTTCAAGAAGTGCGCTACGATCTCTCTGTTTTTGGTTGAAAGAGAAGTGGTTGTTTGGAAGAAGTTTTGCGGTAATTTTCCCCTTCAATCACTAGTGTTGAAATACAGTTTAGATTTTTGTCTTGCTTGAATTACAGCATCCCTTGAGGCATTATCGACGTATATACCGTTGTTGTCGAACCTAAGTGCATCGAGTGAGTAGAACTTATCCTTTGTTTCAAAACGGATAATGTAAGTACTTTGCGCGTTCTTGTCTAAAACTTCTAGGTAATAGAAATAGTTTCTAGGGTTAGCAATACGACGTTGGTTACCAAGGTTGTTAGCGTTAGTTACATCTACTACCACATAGAAGTGACCATTGCTTTCATTTCCGTTCAAGTTAATATTGTTCTTAGTTTGAAAGTTTTGCAGTGCAGTTTTGAGTTGAGTATCTTTAGTATCAGTGCTGCCTAAAACATCTTGAACTTTGCGTACGTTATCAAAGTCGAGGTTAGTGCTAATACCGTTGCTGGTAAGGTAATCGTTAATGCTCTTGTTGAAACTCTTGTCATCAATTTCACGTAACTGATCATTTTGTGCTTTTCAAACAATACTTCTTGTCTGTAAGTTTGCCCGTTCTCAACTAGCACTTTGGAAATCTCTTCTTCAGTTAATATCTGAAGACTAGCTTGTTGTCTTCCTTCTTAAGTAGTGTTTTAGCTACAGATACAACCTTATCTTCTTTCACTTCCAAGCGATCAAGGAAGTCATTATCACGTCAGCCACCATCTTGAAAACCAATTGCATTAACAATAATTTCAGCTTTGTTTTCGTTTGCTAAAGCATTGAGCTTGGTCTTCATTGTTTTCAATTGCTAAGAACTGGAAGTTTTGTCCATCCCACACTTTAACTTCCTTAGTTACTTTTTGTCCTAGGGTAACGTTAGCGTAAATGGCCATGGAAAAGTAAGAAGCTACTAACTTTAAGAATTGATCTCAGTCCTTCGGTTCTTGGTTTAAATTAAGACCTAATTCCTTATTTGCTTCTTGATACCACTTAACTCAACCGGTTGAGTTAATTGGAGTACTTACTTTATTGGTATCTTTATCTAGGCTACTAATGTATTCTTTTACCTTTAGGTAATCAACTTGCTTGTTTTTAATTTGCGTTAAAACGCTGTTAATGCCAGCTTTTTCACTTTCGTTACTTTTGCTCTCTAAACGTTGTTCTAAATCCTTTACTTGTTCAGAAAGGTTGTAAACGTTAGTGATTGCATTAACAAAAACTGGATTTAAAACAAAATCTAAGGTAGGTGCTTGAGTTAAAACACCGTTTCTAGTTGAATTTAGATTAGCGTTTGTTTTAGGGATCAACAGCTTATCTAAGTTAAAGTTTTTGTAACGGACAAAACTAAAGCTTTGGTTTTGTAGTTTGTAATTTCTGTTTTCAAACTTAAAGTTAGTTCCAGTGGTGTTAGTGTCAATCACCTCTTTCACTGCACTACCTAACCCAACATTTAGAGTTTCATCCTTGCGTACGTATGCTTTAAGTTGACTTTCAGCTGCACTTTGATAGTTGGCCTGTGTACTCTTAGCGTTAGCAATAAAGTCAGCTGTTTTTATCGATTGCTGCTGACCATTGCTGTCGGTGTAAAAAAGCTTGGTTCAAGGATCTAACTTAACAAAGAAAGTAATGCCGTGATTAGTAAAGCTCTTTAAAGGACGGTCGGAATAATGAATTGTTGCAGGGTAATCTAATGGATCACTGTTAGCACCTAAGAAAGTGGTTTCAAAGTTTACGTTAGGTTTTTCAATGAAGTTACGATCTTTGTTTGCAAGTCACTAGTTTCTAGTAGTTGTAAAACCAGATTGACGGAACCATAAGAATACTCCTTTCTCTGAGTGGAGGTTGTTGACGTTTTGTAACAGCTTTTCTTTAATGCTACCCCAAACGGTTTTGGAAGTTTCGTTTGTTGCGGGGTCAGATTTTTTTAAATCTGCTTGACGGTGAAGTTCACCCCTTTCAATGAGCACTATCTTTTGGTGGGCAAAGTCAAGGTCAAACCAAGCAGTTCATCTCCATCGATCATCATTGGTTCCAGCTTTTTTTGCACCCCGGTAGTGGGTTTTGTAATACTTGAACTTATCATACCCTGCTCGGTATTTTGTTTCATGTCGAAACGGCGATTTCCAACCGTTATTATTAACAGTGCCCACTAAACCAAAACTCATATCTGACTTAGTTTAGATGTTTACTTCTTTTATTCCTGGAAACTTGTTACTTAATTTAGAAAAGGAAGATCCACCAACAAGCCAACCAAAACCAGCGAGTCCAGCACACAACAGCAACGAATAATTTCGTAAACGCTTTATCCTCATACGTGTAAATTTATAACAAAAAACGCCCCTTGGGGAACGTTTTTAAACAAGTTTAATTATTAGTCATTTTTATTGAATATTAAATCTTCATTTATTTAATGCTTAAAGATTAATTAACTTTTGCACTTGCTTAAGAAGTCCATTTTGACAAAGAAAAATCCCAAAAGGATGGCAATAAAGCTCACGCTTTTGGGATCTTCCTTGTGGTTTAAACCATTTTTTTAGCTAAGTCTACTTAACTTTTCTTGCTTTGAGCATTTGTTCTCAGAAGTGCTTACGACTCTTACGTCATCGGTGTAAGACAACACCACTAGCTATTATTGTTGAAAGTAATGCAGCACTACCAGTGAAAGTAATTGCTATTAATGGTCAGTTAATCTTATTGATGTCTTCTTTATTGAAAATGTTTTTGTGTAGATCAAAGGTGAAGTTATCAATTTGTTTTTGGTTATTTGCCAATTGTACCTTGGTCAATAAAGGTAGTTGACCAGTCTTAATTGGTGCTTGGTATTTGTGTTCTAAAACGGTTTTGAGCTGCTTTCTAAATTCTGTTTGCAATGGAGCGTAGGCATTCTTAATAAAGGAGCTGTCGGTAAACAACAGACTAACAACTGTGTTGTATTCGAAGTTGTTTAGGTTAGAGAAGTCAAACACCAAGTTTTTCTGCTCTCTAACCGTACCATCCTTGTTAGTTTTGGATTTCTTGGTAACAATCCAGTAAGGCAGTTTAATAACGGTACTTTGTTCAGGGTTGGTGGTTAAAGTAATCTTGAAGATGTTTTTGTCTGATTCTGGATCATCACCTTTTTCTTCTGAAACCAAGACATTGTAACCCTTAGCTTCTTCTACAATTTGGTTAATTACACTTGGGTCTTTAACCTCTAAATTACCCTTCTTGTCTAAGGTAAAGTTCGATGTTAAACGTAGCTTTAAATTATTTTTGTCTTCCTTATTAAAGCTCACCCTTTCCTTTAAAAAGAGCTTTTTACCAGTCATTCGCTCAACATAGCCTAAGATCGAACCACTGACATTTTCACTCAAAAAACCCTCATCATTCTCAGCGTTAGGTGTAGATAGTTTAGCTGAATTGTCAGCACTTGCTGTAGGCAAAGTAAGTTCAGCGTTGGTGAGGTTAGCCTTCAAAGCAGCGTTCCAGTTATGAGTATCGAGATAAAGGTTTTGGTTTTGCTTGGCTTGGATAATTGCATCCTTGGACACATTTTTCACATAAAGGCCACGACTATCAACCGCTAGTGATTCGAGTGAATACAGCTTTTGTTTGTTTTCAAAGCGCACTAAGTACGAACTTTGTGCTCCTTTGTCCAACCCTTCAATATAGTAAAAGTAACTCTTAGCGTTATTTGCCCGGCGTTGGTTACCCAAGTCATTGGCATTAGTTACATCCACTACTACATAAAACTGACCAGTGTATGGGTTAGATCGTAGGTTTAAACCATACTTGTTTGCCGTACTTTGAACGCTAGCGAGCTTCGGATCATCACTTTTGCGGGCGTTTAACATCGCATCAACTTTATGAACAGCATTTACTTTAATTTCAGTTTCAGAGATGCCTTGTACCTTTAAATAGTTGTTAATGTCCTTTTGGAAGCGTTCGCGCTCATCGTTCAAATCAGTGCTTTGTACTACAATACTCTTGGTTTGTGAGTTTTGTTTGTCCCAGCTAGCTTGTTTAAAGTTTTGTCGGTAATGGTACTTATAAGAAATTTCGTTGGCTGCTTTTTCTATTTTGCTAGCATCACCGTTAGCAATTTTAGTTTTACTACTCTTAGGGGTTAGAGCTACCTTATTGATAAAACTAGCATCACGTAAACCGCCTTCATCTTGAAAACCAATGGCGCTTACAGCAATCTCAGCGCGGTTTTCATTTGCTAGTTGTTCTGATTGTTTTTCAGTGTTTTCAATCGGGATGAACTCAAACTTAGCACCATCCCAAACCTTCATCCGTCTTTCCTTTGCGCCGTTTAATTTGACGTTAGCAAAAATGTCCTTAGAGAAGTAAGAAGCAATTAAGCTGGAAAGTTGTGTTCAGGTATTAACATCATCGTTCAAGTTTAAACCAAACTCTCTTTTAGCATCAGCATACCATTGTGCAGTATTTTCACCCTTGGATTCAATACTACCTGGATTTTGGCTTCAATTTTTCAGCTTGCCAATAAAATCAGCTACTTGAATGTAGTCAACTTCCTTATTTTTGATTTGTTCTATAACCTTATTGATTGCTGCTTTGTCACTTTCATTGTAAGGTTGATCAGACTTTAAATGCTCCTTTAAATCAAGTGTTTGCGCAAACAGATTGTGAATGTTGACAATGGCGTTTGTCAAAACGGGGTTAAAGTCAAAGGACAGTGTTGGTTGTTTGACAAAAACACCATTCTTAATTGCCGTAGCATCCTCGTTGGTTTTTGGCACTAGTAACTTATCGAGCTTAAAGTTATTGTAAGTGTCAATGCTAAAGGTCGAATCCTTAAGTTTGTACTTTAGCTGTTGGTTGGAGGCTATATCACTCTTAGTGGTGTAAGTTTGACGTACAGCACTACCTAAACCTAAGTCAATACTGCGAGTTTTTCTAACAAGCGTTTTAATCTGGTTTTTATTAACCCATTGGTAATTAGTCTTGGTACTCTTTGCTTTTCTAATGTAATCACTTACAGTAATTGATCTTTCCTGACCGTTTTCCTTGTAAAACAGTTTGCTGTAAGGTTCTAGCTTTAAAAAGAAGCGCGTGTCAGTGGTATGACTACCTCAATCAGGACTGTGCCAATTGGAGTTACCACCTTCAGAAGTGATGTTGGTGGTAAAGGCATTGGGGTTATTGTTGGTATCCCAAAAATCGGTAAAAAAGCCACTGTTAGCCCAGCCCTTAGTACCCTTGGTGTTAAAACCAGTTTGGTTGAACCAGATGAAGATTCCCTTATCTGGATCTAACCGCTCAGCACTGTAGAGAATCTTCGAGCGAATATCACCCCATAAAGGTTTGTTTTTATCCCATTTTCTGGGAGTTGGTTCTGGAACACGCTTGTCAATATGATATTCATCATTTTCCACCAAACCTATGCGTTGGTTGGCATAACCCAAATCAAACCAAGCCACCCACTTTCAGTAATAAGGGTTACTTGCTGTCCTTTCGCGGTTAGAGTACTTGTAATATGTTTGGTTCCAGCTAAAGTGAACGTTTTTGTCGTATTTGTTCTCGTGCCGGAACGCTCAAGTGCGACCGTTATCAGTGTGTCAACCCACTAAACCAAAGCTAGCGCCAGAGTTGGTATACACCTTTACATCTGGTAGTTCACCGTTCTGAATGGAAAGCTGAGCAGTACGCTCAATTTGCTTAAGTGCTTCTTGAGCGTAAAGGCTTTCACTAATGTAGTTATTAACGTTGTTTAAGTAGACCTCTGTCTTTTTACCGTTATTTCACTTTAAATCAAAGCGCTGGGTAATAGCTGGACTTTTACTCTTTAAGATTGAAAGCACATCGCCTTTACCCTTAAGGTAGCGCATGTGAACGCCACCATCGCTTGATAGTGTTTCATTGTTACTTTTAACAACGCTAGTGAATGCTGAACTTGCAACGAATCAACCAAAACTTCCTAATGCAAAACCAAAAAGTAAGAATCGGTACTGTAAACGTTTCATGCTTTATTCTAGCACTTTAAAAATATTTTTGACAATCCTTCTGCGCGTGTATTAAAGCAAATGCTGAACGAAATTGACAAAAGACGCACGGTTTGAACTCAAAAAACTTTTTATGGCGTGGTATTTCACCAAGCATTTCATTCATTTTACCCTCTAAATGAAGAGTTACTTTGCTCTGCTTATCTCAATATAGTTAAAGTAATGTGTATAAGCGTAGCTAAAATAAAAAAACCAACTGAGAGCAGTTGGTTGGAATCAATAATTTGGCGCGCCCAATAGGACTTGAACCCATAACCTTCTGGTCCGAAGCCAGACACTCTATCCAATTGAGCTATGGGCGCTTCTTATAGTTAATTCTATTAAATCTCTGAGAACTGCGCAAATCTTTATTAAAATTAGCTGACTTCAACACAGTCGCTCTAATACATGTCAAAGGATAAAAAAAATAAGGTTGAACAGCTTGAACCAGTAGACCTTTTTGAGCGGACTAAGCTAGAGGACACCCAAGTTTTAAATGATGTTGAACTAGATGACATCAAGAAACTAGAAGAGTTGAAAAAAGAATTGGAAAACACCTTTGAGCCACGTACCAGAATTGAAATTAAACGTGAAATCAAAGAGTTGGAACGTAAACTAAGAAGGAACCGTTAGTGAATGTTTGTTAACCTCCACACTAACTCTTACTATAACTTCCTCAACTCCACACTTTCACCGCAAAAGCTAGTTGACCTAGCTGTTCAAGACCAACAGGTAGCTGTTTGTTTAACCGATCCCAACTTATTTGGTGCTACCGAGTTTTTTTTGGCCTGTCAAAAAGCGCACATTAAACCCTTAATTGGGTTGAGTGTTACTGTTCGACATTACGAACAAAACGTTAATTTACTAGTAATTGCCCAAACCAACCGGGGTTATCAGAACCTAATGTGCTTAGCGTTAGTTAAAGACCAACCAGATTTGCAATTAGAACCCTTTTTGGATGGTAATGTGGTAATTATTTGCACACAAACTGAACTGCGCCTCAACACCGCAAACCCCGTTTACTTAGCACATGGTTTGAGTGGTCGTTATCCTAAAATTGCAGTAACACAAAAACCAGTTAAGTGTCAAAACACCAATAAGGACTTAACACTGCTCTTAACACTTAAGCAAATTAGTCAAATTAACAGCGAACACTTCCAGCCACTGGAATGAAAATTAAGCCGTAGTTTAAACGAAATCCAATTGGATCCACCATTGTTGCAACAACTCCGGCACCAACCTTTTTTAAGCCAAAAGGCAGCACAGCAAATTTTTAGCGAAGAGGAATTGGGAAATCTGCAAAAGCTAGTGGAGCAAAGTCAATGAGATTTAACCAAACTCAAAGCGTCATCGTTACAAGTGTCACACAACGATGCACAAATGTTAAGCGAGCAGTGTCAACTAGCCTTAACTGAATTTTTAAAGCTCAATCCGCAATTAAACAAACAGCTCTATGAAGAGCGGTTAGCCAAAGAATTGGAGATAATTAACTCACTCCACTTTGCTAGTTACTTTTTAGTGGTGAGCGACTTAGTTCAGTTTGCCCATAATAACGATATCCTCATCGGTCCTGGTCGAGGATCTGCAGTGGGTTCTTTAGTGGCCTTTCTGCTCAAAATTACCCAAATTGATCCAGTAGCCAACAATCTCATTTTTGAGCGCTTTATTTCCCGTCACCGCCAGGGCTTACCTGACATTGACATTGACATTATGGAAACTAAACGTGATTTAGTAATTGACTATGTCATGCAAAAATATGGCCGGGAACAGTGTGCACAAATAGTTACTTTCCAAAAATTTAAGACCCGCTCTGCGTTGCGTGATGTCGGTAAGGTATTTAACCACATTGAGGGTGCTGAAGATTTACTGGGTAAACTACCAAAAGACAAAAGCTTATTGGAGCTTGATGTAAACGGTGTTACTGATCCGGTTTTACAACTTAGTTTAAAGAGCTTTCGCTTGTTGTGGGAGGTGGCGCGTGAAATTATTAACTTTCCGCGCCAACCAAGCATTCACGCTTCTGGTGTTGTGATTGTCACTGAACCTTTAATTACCACGATCCCTTTAATGGTGGGTAATAACAACAACTACGTCACCCAAGTGTCGATGGACTGGTTAGAATGGTACAACCTCAATAAGTTTGACTTATTGGGTTTAATTAACCTCACCATGATCCATGAGGTTGTGCAAGCGGTAAAACCCAAGGAAGTTAGCGTACAGCAATTCCTCCAACAAATACCGCTAGATGATGAAGCTACTTTTACTAACCTCACTAACCAAGCCACTTTAGGTGTTTTTCAACTAGAGTCGTTTGGGATGAAAAAGGTGCTCAAACAAATTAAACCGCACAACCTCCATGATTTAGCAATTGTTTTAGCACTTTACCGCCCCGGTCCACAGGACAACATTAATACCTTTATTGCTAACCGTAACTTAGGGTTTGACACATCAGACATCGATCCGCGAATTTTGCCAATCCTCAAAGAAACTTACGGAGTGTTAATCTTTCAAGAACAGGTTATTAACATCGCCAAAACTGTTGCTAACTATTCATTGGAAACCGCTGATAGCTTTCGCCGTGCCATTTCCAAAAAAAACTTGCAAGTAATTCAGGACAACATGCGCAGCTTTTATGAAGGCGCGCTAGCCAATAACTTTAGCTTAAAAGCAGCGACTACTATCTTTAATTACATCCAGCGCTTTGCTGGATATGGTTTTAACTTGTCCCACGCCTTGGGCTATGCTTTATTGAGTTACTGAACAGCATGATTGAAGACACATTACTTTGAGCAATTTAACCTGTGGTGATTAAACCACGAACAGGGCAAAAAGGAAAAGCAAAAACAGCTCTTAAACGAGTTTATTAGCAGTGGTTATGAAATTTGCCCACCACTAATCAATAAGGCTAAAAGTGATTTTAGTGTTCAAGATAAAAAGCTGTATTTAGGCTTTAAGTTAATCAATGGTATTGGTGACAAGCAAGCCCATGCCTTGGAACATGTTCAGGAAGTTTTAAAGCAAAACCCCAATTTATCCTTAATAGCTACCGTTAATTTGTGTTTGAGCAAAACGGTAGGGGGTTTAGAACTTAAAGACATTACATTGCTCCAACAAGCAGGTTGTTTTAATTGCTTTAATTACACGGTTGACTTTAATTTGGCCAAAAGCTTTTGGGTGCAAAGTAACCATGAATTATTTCCAAAAATACCACTAGATCAACCACCCGTAATTAACTGAAAGAGCTTTGGTTTTTAATTTATGAAAAATGCAATTTTAATTGATGGTAACTCACTAGCCTACCGTGCTTACTTCGCTACTTGACAACAGGTGGAGTTTGCCAAACTACACAACTTACCCTTTAACAATGCGATTCGCACAATGTTAATGATGTGCTGAAACTTACTGCAGTCAAAACAGTATGACTATGGTGTTATTAGCTTTGATACTAAAGCGCCCACTTTTCGTGATCAACTTTACAGTGAATACAAAAGTAAGCGCAGTAAAACACCGAGTGAACTGTTAGTCCAAATTCCTGTTGTTAAAGAATCACTGCGTCATTTAGGTTTTTTAGTGTGTGAACAAGACGGGTTTGAGGCAGATGATCTAATTGGTAGTTATGCGAGGTTAATGACACAAAACAATGTGGCAGTAGACATCTACAGCTCTGACCGTGATTTACTACAGTTAGTCGATAGTATGACTAGTGTTTGGCTTTGTGTAAAGGGCACAAAAGAGATGAAGGAATATAACACTGACAACTTTGCTGAGCAGTTTTTTGGCTTAACACCAAAACAAGTGATTGAATATAAAGGTTTGGTAGGGGATAACAGTGATAACCTCACTGGCATTAAGGGAATTGGTCCGAAAAAAGGGATTGATCTACTCAAACAATACGGTACCATTGACAATATTTTTGCTAACTTTGACAAGCTATCCAAAGCATTGCAAACGATTCTCCAAGGCCAAATTGACACTGCTAAGAAATTCAGCTTTTTAGCGAGCATTAAAACAGATATTAAACTTAATGACGATATTGTCCACGCTGCTTTAAAACCAATCGATAAACAAGCTTTATTAGAGCTTTTAGATAAATATGGCATTAAAGCACTAGCGCAAAAATTTAGTCAACTGTAATGCCTGAACTACCTGAAGTTGCCACTGTAATTACCGAACTTAAGAGCTGTGTTTTAAACAAACCTGTCAAGCAAGTTAAGGTTCACTTAGACAAGGTGTTGAAAAATACTAATGTCAAGCAGCTTAACGATGCGTTAGTCAACCACAGCTTTGTGGACATTAAGCGCCGCGGCAAGTACATTATTTTTTGTTTAAGTAACGGTTTGTTTTTAGTGTCACACTTACGCATGGAAGGCAAATATTTCTTTGAAGCTAAAGGCAGTCAGTTTGACTTAAACCATGTTTTAGTCGAGTTTTTATTCCAAGATGGTGATCAACTTAACTATCATGATACGCGTCAATTTGGTACCTTTCACTTGTTCAATAGATATCAGTTTGAAAACGCACGTGAATTGAACAAATTAGCACTCGATCCCTTAGATCAGGAATTTAACCATCAAGCGATCTTTAACAAAGGCCATAAGTCCAACAAGAAGATTAAAACCTTTATTTTAGATCAGACCAACATTAGCGGTATTGGCAATATTTACGCTGATGAAATTTTGTTTGCAAGTAAGATTCACCCTGAAACTTTAGCTAAAAATTTGAACTTATCGCAGTATCAGCTAATTTGTCAAAATGCAACTGATATTTTAAAGAAAGCAGTTGAAATGAAGGGTACTACCATTGGCACTTTTACCTTTAAAAAAGATCATACCGGTGGTTACCAACACTTCTTGAAAATTCATGGCAAAAAGGGGAAGCAATGTCAAAGCTGTAACACCACTATTATTAAGAAAAAGATTAATGGTCGGGGCAGCTATATTTGTGAAAAGTGTCAAATACAGCGTTAATGGTTAACTGTTCCAGTTAATGCCAGAATTATGGCTTTATAAAAAAGAGCTTAAATATCTTAAACTTAAGCTCTTTTTGATCACTTAAATTCACCATAAAGATTGTTTTTAATCCTTAGATTAATCCACGCTTGTTAGAAAAAATATGAAAAACAAAATTAAATACGTTTACTCCGACCTGGATGGCACGATTGTGAGCTGAAATCCTAAAAACCAGTTTACCCATCAGGGCAAAACTTACAAAAATCTCCATGAAGTTAGTCACGCTACCGTAACTGCTTTTAAACAATTACAAGCTCAAGGTATCAAAATTGGAATTGTGACTGGCCGTGATTACTGTCGCGTTCGTTGGTTAGAAAAGTACCTAAACACTGATCTACCAACAATTACCCTCGATGGCGCCATTATTTACTTCCGCGATGAAATTATTCGGCAAGAAGTGCTCGATAAGGAATTTATCCATGGCATTAACCAGATTGTCAAACGTTACCCTACAGCAGCCTTTAAGCTCAACATGGGTTGAGGTAACTATTACACCTGCAATCCTTCTTTAATCTTTGAAGGTGACCATGCTTACCGTGAGCACTTTAATGCTGACAGTAAGTTTTATCGCAAAGAGATCGACAACACGGTTGATTGAGATATAAATAACATGAAAGTAAATCAGGTTTACTTTGACACATTTACTTGCCCTGAACCAATGATCCAAGAGCTCGATAACTTAGTTGAAAAAAGTGATGTAACCGCCAAAAGCTACCGCCATTCACTTTACATTATTAAAAAAGGGGTATCGAAGGCTAGTGCACTACAAAACCTCCAACGAGACTTTTTAGTGGAAATGAAGCCTGCTAACACGATTGTCTTTGGTGATGGGGATAATGACATTGAAATGATGCAGTGAGCTGATCACAGTGTGTCGTTAACTGGTAGTGATCCCGAGTGTTATAAGTTAGCTAAGTACCACACCGACAGCGTTGACGATGATGGGATTGCCAAATGGATTAATAAAAATCTGTTATGTTAATCGCTGTTGTAGGCAAAGCTGGGGTTGGTAAAACCACCGTTTTGCAATACATCGCTGACTATTTTCATTTTCCCGTTTTTTTTGCTGATCGGTTTATCCATCAACAATACGCAAATGGTCAAGCTGGTTATGCAATTGTAAAACAGCAATTTGGTGCTCAGTTTGTCAATCATGAAGCAGTTGATCGCAAACAGTTAGCACAATACGTTTTTAATCAACCTGATGAGTTAAAGCGTTTGTCCAACTTAACCAAACCCCTAGTACAAGAGTGACTCAATCAATTAAAAGCGCAATTCCAAGACAAAATTGCACTTGTGGAAATTGCGGTGATGCTCAATTACTGAAACGATTACCGCCCTTTCTTTGATGAAGTAATCCAAATTGAACGTGATGCTAAGATAGTAAAACAAGCACTAAAAGCACGTGGCGTTGATGTAGAGCAGGTGCAAAAGTTAATTGCTGATCCGACTTACCCAATTCTTACAGTAATCAATAACAGCACTGTAGCTGAGTGTGCTTTACACGTTACTCAATTTTTAGAAAGCATTGCAAAAAGTGATAAATGCCATCATGGTCATTATCAAACTCCGAAATAAAGGTCGCGTTTTGTTTTGCTAAATCACTACCGTTAGCCATTGCGACAGAGTACTTGACAGCATTAAAAATTTCCACATCATTGTCCCCATCGCCAAAGACCATGGTCATTTGGGGATCTAAACCGGTTTGCTGTTCTAATGCTTTGAGACCGTATGCCTTATTGACATCTTTGTGCATAATGTCAAGCGCAAAGGTCATAGAACTGACATAGCTAATGTTTTCAATCGCATCGAGATGTTTGGTTATTTTACTAATGCGACCCCTGTCTTCAGTGATTAAAAGAATCTTGGTAATGTTTTGTGTGGCAAAATCAAACTTGCTAGCATCAGTTAACGGATTAATGGTAAAGTTATTTTGTGTAATGTCAGGTTCTACAATTTTGCTTTGAGTAGTGCGTAACCGTTTGCAAGGTATACCGGGTTTTTCAAAACCAAACACCTTTTTATCAGTGTAAATGCAAAAGCTTTCTTCCAATTGCAACAGTAAACGCATAATGGGTACCACACTTTGGGGTGCTAACTGGGTCATATGCAGCGCTTTTTCCCGAGCAAAGTCATAAATTAAAGCACCGTTAGAGGAAATAACCGGTAGATCAGGTTGAATAAACTGAGTGGTATTGCGGATTAAGATATGACTGCGCCCAGTCGCAAAGGTAATTTTAAACCCCTGCTTTTGCAACTCTTTGAGAAAAGTAACAGTTTGTGGTAGTGGTTCATGACCCCAAGACAATAAAGTACCATCGAGGTCAAAAATTAAGTTTTGTACTTTTGGTTTCATAGCTATTCGGATTTTAAAAGAACATTAATAATACGATTAGGGACAAAAACAACACGCGCTAAGGGTTGGTCGAACAACGCTTGGAATTCCGGATCTGTCTTAAAGGCTTTTAAGACAGTCTGTTCATCAACCCCTTTAGCAAATTCCTTGGTACCTTTGAAGCGACCGTTGACCGAAAGAATAATTTTAACCTTATCGGTTTCAAGTACGCTGTTATCAACATTTGGTAGTTGAGCATGAACAATAAACGGCTCCATCCCTAGTTTAGCGTTTAATTCTTCCGCTAAATAAGGTGCAAAAAACGATAACACCGTCAAAAAGCCCTTTGCATAAGCCAGGGTTAATTGTTTTGTTTTGTAAAGCAGGTTTAAAAAGATCATCATTTGGCTAATTACCAAATTGAGTTCTTGTTTCTCTAAGTGCTCGTAACTACGCTTTAAAAAGAGGTTATAACCATAAACAGTTTCTTGATCTACCGTTTCTTTAATAATGTTTTCCTTGTGAAAGAAATTGTGCACCCGATCAAGTCAACGCCGCATTCCGTTTAACCCATCATCCAATCAGGTCAACGCCGCGCTAATCGGCCCCATAAACATCAAGTAAAGTCGCAAGGCATCAGCACCATGAGAATCAATTAGTGGCGTAGGGTTGATTGTGTTGCCCTTGGATTTGGACATCTTTTTGCCATCTGGTCCCAATACCATGCCCTGGTTAATTAACTTTTTAAAGGGCTCTTTAGTGGTGACTATTCCCTTTTGGTACAAGAATTGGTGCCAAAAGCGCGCGTACAAGAGGTGCAACACGGCATGTTCGGCACCACCCACATACAGATCCACTGGTAGGTAACGCTCAAAAAGATCTTTCGCTTCCCTGGAGTCAATTGGCCAAAAGTTTTCGTTATTAATTAACATCAAATAACCCAAGTAATACCAACAAGAACCAGCCCACTGGGGCATAGTGCTCGTTTCCCGAGTAAAAGTTTGATTGCCTTGTTTCACCTTAGCCCACTCCTGGTTTCGTCACAATGGTGGGTTAGTCGAACCATCAGGTTGATAGTTAGCCAAAGCTGGTAGGGTAACGGGGAGCTCTTTAACTAAATGGGGTTGGTTGTTTTCGTCAAACAAAATCGGAAAAGGTTCACCCCAGTAACGTTGACGGCTAAAAATCCAGTCACGGAGTTTGTACACCGTTGTTTTTTTAGCTTTTTGACGCTCGACTAACATTTGCGTAATTTGGGTGTTAGCAGTTTGGGAGTTGAGCCCGCTAAACTGCCCGGAGTGTTGCAAGCGTTCCTGTTTATCAATGACGGTAATAATCGGTAGCTTTTGCTTCCGCGCGAAGAAGTTATCGTTTTCGTTATGCGCTGGCACACCCATCACAGCATCCGTACCATAACCTTCCACTACATAGTTAGCTACATACAACGGAATGGCAGTATTAGTTGCTGGATTAATTGCTTTAAGTTTTAAATCAACCCCGTCATGGAGCGTCTCAGGTTGCTTTACAGTGGTGGTTTTCTGTGCTTGTTTTTGGAGAAACTGTGCTATCTTGGGGTTGGTTTTAGCTACCTCTTTTACTAAGGGATGGCTATTGGAAAGCGCTAAAAAGGTTACACCAAAAATAGTTTGCACCTTAGTGGTAAAAATGGTAATCGTTTCCTTGGAGTTTTCTATGTCAAACGTTACCTCTACCCCTTCACTTTTACCAATCCAGTTGGTTTGCATTTCCTTAATAGATTGATGTCAATCCAGCTCAGCCAAACCATTGAGTAAACTTTCAGCAAAAGCAGTTATTTTCAACACCCATTGCTTCATTTTTCGCTTGGTTACCGGGAACTCGCCACGCTCACTCACCGCATTACCATTACTGTCAATCAACACCTCTTCGTTGGCTAAAACGGTGCCCAATTCAGCACACCAGTTGACATCAATCTCCTTCACTTCAGCCAAACCGGCTTCAAATAACTGACCAAAGATCCACTGTGTGTACTGGTAATAGCGTGGATCAGTTGTCTTTAAAGACAGCTGGTAGTCATAATCAAAGCCAAAGGCCGTTAACTGCTCAATGAAATTAGTAATGTTTTTGTCAGTTCAGTCACCAGGATGCTTATTGGAGGCAATCGCGTATTGTTCTGCTGGTAACCCAAAAGCATCAAAGCCAATCGGGTGAATCACATTAAAACCCTTCGCTTTATGATAACGACTCAAAACATCGGTAATGGTGTAAGCGCGCACATGTCCCAGGTGTAAACCAGCCGCTGAAGGATAAGGGAACATGTCCAAGATATAGTATTTGGGCTTAGTTTTATCATCAAAAAAACGGTTAACTTGCTTCGTTTTTCACAATTGCAACCATTTCTCTTCCAGTAAGTTGTGGTTATACATCTAGTTTTTTTTGAACGAGCGCTTAATTAAATACTTAATTAAATGGTAGAACAGGTCAGCTATAATGGCTACAGCAATCCCAAAAAGTAAACCAAAACCAACTGGAATAAAGAAGTGCACCAGGGTTTGGAACAGTTTTTGCTGTTGCTCTACAGTGCCTTTAAACCATTGATTAATATTGGGAATAATTTGCACAATTAGCCCACTAGCATCCTTTTCAGCTAGTAAATTAGCCCACTCCTTAGTATTGTAGGTATTGAGCCAAAAAGAGCCAATAAAAATAATAAAACCAACCGCGACAAAGAAGAGGAAAATCGCTAGTTTAATGAGCCGTTTAAGCAGCATGGTAAGTGGAAATTAAGCTAATTAAATTATAAAGCTAGATAATTTCCCAGTCTAAGTTTTTTGACTTTAATACCTCTAGTACTTGTTTGGTGATGGATTCTACTGATTGGTTAGCGTTTACTATGCAATGGCTAATACCAAAGTCATAACAAACGTTTTTAATAAATTCACCATAAATCTTGTACAGGTTCTTAAAGTAGTCCTTATTTTTGGCAAAGTTATCAATTTCTACTTTACGGTTACGTTGAAACAATCTTTGTTCAAACAACTCCCAATCACCTTCTAAGATAACGTACAAAGCGGGAATACCATGTTTAAAGATTAGTTCTTTAACAATCCCATTCCACAAGTGGTTATAGTAAGTAAAGATGGAAGGACGGTGGATGTTTTGCTTGGCGAACAATCAATCTTCAAAGATCGAGCGGTCAAAGATAGTTGGTGTTTTATTGTTACATTCCTTGCGGTATTTATTAAAGCGGTTTAAGGTAAAGTACAGTTGAAATAAAGGGGCGTAGAGCAACTCGTCGTTACGCTCATACATTTTGGCTAACAATAAATCAGCTAGCTGGTCTTGTTCTTCCAGTTCGTAAATCACCTTAGAACCCTTTAAGTGTTTAGCTAAACTTTCAGCTATCGTCGTTTTACCAAAAGCAATCATGCCACCAACCACAACGGCGTTAGCAATCTTAGCTGGTTGAAATTTGGGTTTTAAGGGAGTCTTCATAATACTTAGAACGTGGTTACAGTGCAAAGTATTATGCAATAAAAAACCATCACACTCATTAAATAGTCTTAATGCTGCTCCATTTCTGGCCTGACATGATTCGACCGTAATGATGTGACGGCTCTAAATTCTAACACTTAACTGCTTTTAATCAATTAATTTGCTTACGCGCTTAATCGAATTACGCTTCGGCTGGGTCGCGCCGCGAGGTAGATCTTTATTGGTTGTTGGTGGAGTTTCTTCCACTTCCATGTCATCATCCATATCATCGAGTGTATAGCTAATAACTTGGGTTTGCGGGATAACAGCCTCTTGAGGACGGTGGAATTGCTGTGAAGTGTCAGCTAGGCTAACGGTATGGGTCTGACCATTAACTTGCGCGTTAGTAATTTCACCTAACTTTTGTTCCAAGCGGTTTTTGAGTTTGTTGTACTGCTTGACCAAATCGTTGTTAGCGCGCTGAAAGTCACGCTTTTCACTGGTAAGTGAATTGTTAATTTCCTGAATCCGGGCTAAATCGACTTGCAAGTCACTCACCATCTTTTCGTACTTTTGGTTTTTAAACAACACTAGTTTGTGCTGCTTTTGCAACGCCTCATTTTCATCAACCGCACTTTGCAAGTAAGTTTGCAGTTGGGTAATCTCACTGTTCTTTTCGTTTAACTTACTTGTAAAGATGGCCTGGATTTCCTCATCACGCCTGTGAATGGCATCAGTACACTGCTGCTCGTACTCCTTCAACGCTTCTTCCAGGGTTTTGTTGTAGTGTTCCTCAAGCTTGTGGTTGTACTCCTTGGCTTCATTGAGCTGCTTTTGATACACCTCTAGTTGCCGTTCCTTTTCTTTAAATAGCTGCTGCATCTGGATAATTTCCTCATCACGTTGACCTAACTGGATTAACAGCTCACTGTTTTGTTGGCTCTGGCGTTTAATTTCCCCTTCATACCGTACTTTGAGCTGTTCTTGCAACACCAAGATCTTCTTTAAGCGATCCTTCTCTGATTGCTTTTCGGGGAGAAATTTCTGTGCTAAATACAGCAATGTTTGGTTCCAGTCAAGGTGTGCAGGGTTGGTTTGGCGTAACTGCACATAAGCCTGACGCGCTTGCTCTAGGAAGTCCTGGAGCTGTTTTTGACTCGCTAGTGGAAACTTTGAAAGAAAGAAGTTAATTGTCTCTCTTCTTTGAAAGTCATCAAAATCACGGTAATACTCAAAGTTAGTCATTTAAGGCCTTTTCACGGAAAAAGATAGTTACAGCATCAGGATTAGTAAAGTCCAAGTCACAGTGGTACTTGACATACTGCCCAACACTGTTAAAGATTGCTAACACAAAACTAGTAAACAAAAAGAAGGGGATTTTGCTCGAATAACACGCATAAAAGTGCTTAATCTTAGCACGCATAGTACTTTTAAAAGTTTCTACATCGCTAAAATCACGCGGATTGAGCAAATAATCCTTGACATCACTAACCCGTTCCACCACCCAGTCATACGGAAACTGGTTAGGGTCAAAGTCGTGTTGGATGCGTTGAAAGTCAGCTGCGTCAATTTGGTCTAAAGTTTCAATAAAACCGTTAATGTATTTCTCAAAGAAGTTACTCATCCTTTTTGTTATCAAAGATAACTTCGAGCATTTGTTGAGGTGTTAAGGTACCAAAGTACTCCTTAAAGTTATCAATGTTACCCAATATTTTAGCAAAGGTTTGGTAATCGTATTTTTGTCCTACCAACTGCTGGGTAACGGGGGTAATGTCGATAACACTCAAAAAGTCCCCATAGAACTTAATGTCGACTACTTTACCCTGATCTACCTGCACGTTACATTCAAAGATGCCCGCACCCTCAAACCGTTTCTTGTTTTTAAAGTTGTAGTCAGCTGTTTTACCAAAGTTTCAATCCCAGGATTGGAAGTGTTCCTGTGCACGTTTTTCCACCATCTTAATGCTGCTTTCATCCAAATGAATCGTTTCAGCATGTTCGGTTTCGGTAAAGAACTGCACCATCTTTTCCAAGAAGGTTGGGGTAGAGAGGTTGGGGAGGTATTCTTTTACGTTAACTACCCGTTTTTGGACACTTTCAATTCCCTTACTTACCATTTTTGTTTTATCCACATTGAGGTACTTCGCTAGCTTGGTAAAGTCGGTATCAAACAACAACGTACCGTGGACCAACACGCGGTCTTTGGAGAGGTATTCCGCTAAACCAGAGAACTTCTTGCCCTCAATTTCCAAGTCATTGCGGCCATGAAACTGTGCGGGTACACCTACACTGTTTAAGAACTTCACCACATTACGGGTTGTTTCTTCATAAGCGTTTTCCATTTCCTTTTTTGCTTTGGGCAAAATAATTGAGAAACAGATGTTACCCATGTCATGAAACACCGCCCCACCTCCAGAAAAACGGCGAAACAAATTAACCTTGTCCTTTTCCACTTCACTAAGGTTAACCTCAGCGTACGTATTTTGGTTGCGCCCCACCACAATCGTGTTGGCGTTTTGCCAGAAGTAAATGACTTTAATTTCCTCGCCCTTTTTAAATTCGGTGAGTAGCCATTCTTCTAACGCTGCGTTGAAGTAAGGAATGTTTTTAGGGGAAGTGAGAATGTAAGTTTTCATATTCAGTTGTTGATTAGTTAGACAGTTTATTTAAAGTGATCAAAAAGAGCTTTTTTGCACACATCAGCGATCATTTCGTTGATGGTAGGATGTGGTGAAATGGAGTTGGCAATGTCAAATACTGTCAAACCTGCACCCATAGCCAACGCTAGCTCGGCAATCATGTCACTAGCAGTAGCAGCAATAATACAGCAACCCAAGATCTTACCGGTTTGTGGGTCAAACATCATTTTGACAAAACCGTTGGTTTCATTGTCAGCGATTGCTTTTCCACAGTGCGCTAAAACAAGATTGGTTTTGACATAAGGAATGCCCTGTTTTTTCAACTCCATTTCGGTGTAACCCACCGATGCTACTTCCGGATTGGTGTAAATACAGGAAGGGCAAGTTAACTTTTGCGCTGGTTTGACCTGCTTCTTGTTTAAGATATGGTTCACCGCATACCGCCCTTGTTGGTAGGCAAAGTGGGCCAACATTAACTGGGCATTGGCATCGCCAACTATGTAGATGTTGGGAATTGAGGTTTGGAGATCTTGATTGAGCACAATCCGGTTGCGCTCATCACGTTGTAAGTTCAACCCATCAAGACATTCCGTGTTGGGAATGCGACCAATCGAGACCAAAATGCGATCACCAACCACACTGCCTTCCTGCCCGTTTTGCGAATAAAAGACTTCATTATTGTTAGCACGGGTTACCTGTGCGTTGGTAATAATCTTGACATTCTTGGTTTGCAAGAGTTTAGCGACTAAATCTGATACCTCAGTGTCAAAGATTTCCAAGATCCTATCCACCCCTTGTAAGATAGTCACTTCACTACCCAATGAAGCGTAGAGAAAGGCAAATTCAATACCAATCACTCCACCACCAACAACAACTAACTTGCGTGGTACCCCTTCCAAAGAGAGCGCTTGGGTCGAGTCAATTACAAAACCGTTTTGACGCGCTTCTGCAAAACCCGGAAGCGTTAAGTAACGGGGTCGTGAACCAGTTGCGACCACAATGCTTTTGGTGGTGTAAGTTTTACCCGCTACCTCCACGGTGTTGGGATCCAAAACCTTGGCTTCTCCCATTACCGTTTCAGCTTTAGCGGAAGCAATAATCGCTTTAACCCCACCAACTAACTTACTCACTACCTTACCTTTTTGCTCCAGCAATTGGTTTCAGTTAAGTGCTACTTGACCATTAATTGAAATACCATAGTCCTGCGCATGGCGGAGGTAATCGACAATCTTGGCCCGTTTTAACAGTGTTTTCGTTGGAATACAACCAACATTTAAACACACGCCACCAAAGTATTCCTTTTCGACAACGAGTGTTTTAAGTTTATGCTTACCCGCGTACTCAGCGGCCACATACCCAGCGGGACCAGCCCCTATAATAATGAGATCGTAATTCATCTAAGCTACTTCTAAATCAATTAATTCTTCGATTTGCTTGGCAATTTCCTTACCAAAGCGACCCACATCAGCGCCATCGACCCAGCGGTGGTCGGCAGCAATTGTTAACGGTAGGATGGTATGAACGGCTACCCCACCTTCAGCACGCACAACACGTTCTTCCATGTTACCGGTAGCGACAATACACATTTCCGGGTGCTTAATAATCGGGGTACCAAAAGCAGCGCCTAAAGAACCAAAGTTAGTTACCGAGATCGTTCCCTTACTTAAATCAGGAAGCTTAATTTGCTTACTGCGGGCGCGGTTAGCAAGATCAACAATGTCCTTGGCAATGTCGACTACCGACTTCGTTTGTGCTTGCTTAATGTTAGGCACAATCAGACCATCGGGAGTGTCCACGGCAATACCTACGTTAACATCCTTGTTTAACACAATTAGGTTGCGTTCCTTGTCATAACGACCATTGAAAACAGGAAACTTCTTCAAAGCATTAACAATTGCTTTAACAAAGAAAGCAAAGAAGGAAATCTTCATGTTGTACTTGCTTAAAGCTAAGCCGTTAACACTCTCACGGTACTGCTTTAACTTAGTAGCGTTAACATAGAAGGTGAGAATCGTAGCGGGAATGTTTTCGTGTGATTTCACCATTGCTTCAGCAATTGCTTTACGCATTGTCGTGATCGCAATCGTTTCCTCACCACTAGGTGCTGCTGCAGATGCTGGAGCTGGAGTGGGAGTTGGAGCGGATGTAGGTTGTACTACAGGAGCTGGTGTTGGTTGGGGCGCACTTGGCTGCACACCAAAAATAGGAAACACACTGTTCGATACCTTAATTTCACCCACTACCGAGGCACCCGCCTCTTCCACTACTGGTTCGGTGGTTACAGGTGCTGGTGTTGGGGTAAATGTTGGGGTTGGGGCAGGAGCTGGTGCAGATACTGGTTGGGGCGCAGCAGGTGCAGCAGCACCAGCGCCATCATCAATAACGGCCATCACCTGACCAATGTGCACCACATCACCAACATTAGTGGTAATGGCGGTAATAACCCCAGCATAAGGGGATGGAAGTTCGGTGGTTACCTTATCGGTTTCCACTACAAAAAGGGCTTCATCGACCTTGATCGTATCGCCCACTTTTTTGAGGATTTCAGTTACTTTACCCTCATGTAAGCCCTCACCAACATCGGTGAACTTAAACTCATTTGCCATGATATAAAAACTGTTTTAATTTTAGATTTAAAGCAAAAGTAATGCAAATAAAAAACCAGTCTTACGACTGGCTTTTTATCTAAAACGAAATTTAGAGAAATTTCGCCTTATTTTTGGCAGCATTTGCAACCGCCACAGTTGCATTTACTGCAGTTAGCTGTTTCGGTTTTGCAACCACAGCATTTCTTGTCTTCCATCTTAATATGAAATAAATGGTGTTCCCTAGCCTTCTCAACTTTTAGTTAAAAAAACTAGGTGCAATTATTCTAACAAACCCCAAACTTATTTCAAATGGCTTGATTTAGAAAAGTCGTTAGTTTACTTTAAAAGTTGGTTAACAGCTTCCAAAATGCGGGCATTTAAATTGAACTGGTAGTGTTCACCCCGTGCTAACGGTACCACAATGTCCCAACCGGTTACCCGTTGTGGGGCTGCCTTGAGATAGCTAAATAATTCTTCAGTTACCGATGCAATAATTTCCCCACTGGTGGTAAAGGTTTTAGCCGCTTCGGTAACTACGAGCAAGCGCCCGGTTTTCTTCACGGAGTTAAACACCGTTTCCTTGTCTCAAGGGGAAATGGTACGCAAGTCAATGAGTTCAATGCCCTTGTCCTTCAACTCACCCCCATAGACCATGTTAATTAGGTCAAACATGGTAGGACCGTAACTAACAATAGTAAGGTTGTTACCCTGGCTAATTAAGTTAGCTTGACCAATTGGCACAGTGTAATAATCTGCTGGAATTTCCTGACGGAAGGCACGGTACAGCTTCTTTGGTTCAAAGAAGACCACAGGGTCAGGTGATTCCACTGCTGCTAAGAATAAGCCCTTGGTGTCGTAAGGGTTGGACGGCATCACTGTTTTAAGTCCGGCAATTTGACCGTAAATCGCTTCCAAGGTCTCACTGTGGTGTTCAAGTGCTTTAATCCCACCACCCATTGGCATGCGGACAATAATCGGACAGGTATACACACCGCGAGAACGGTTGCGGATCCGTGCTGCGTGGGTAAAAATTTGGAACATTGCGGGGAAAGAAAAACCGGAGAACTGGATTTCCACGATTGGTTTAAGTCCACCAATGGCAGCACCCACACCAATACCAGCCATAGCTGCTTCGGCAATCGGACAGTCCCAAACACGCTCCTCACCGTACTTCTTTTGTAAACCCTTAGTAGCACGGAACACACCACCTTCAAAGCCAGCATCCTGACCGTATAAAACAACGTTGGGGTCGCGCTCTAACGCTAGATCCATTGCGTTACCCAACGCTTCAATGTTATTTGCTTGAATTGTTTTTGACATAGTGAATTTATTAATGTGAAATTAGTCTTTAAAGTATTTTTTAGCTTCAGCCTTTTGGCGCGCTAGATCAGGAGTCAACTTTTCATAGTTATAATCAAACACTTCATCCAAGGTAACTGGCGTTTTGCTAACCATTACCTCATATGCAGCTTGCACTTCTTGTTCAATCTTAGCTACCATTTCCTCTTCTTGTTGTGGGGTTAGAATACCACGGTCAAACAAGAAGTTACGTAACCGCTTCACAGGGTCACTCTTCATCGCTTCCGCTTCCTCTTCTTTAGTACGGTAAATGGAAGGGTCATCGGAAGTGGTGTGGGGACCTTGACGTCAAGAGAAGAACTCAATTAACACTGGTCCATTACCGCTTCTCGCATAGTTAGCTGCTTCGTGCATTGCTTCGTAACTCGCAATTAAGTCATTACCGTCAACGCGAATACGAGGAATGTTAACCGCAATAGCCTTGGTGGAAAGGTCACTTACAGCGGACTCTAATTTAGTTCTGGTGGAAATGGCAAACTGGTTGTTGTTAATACAAAAGACCGAGTTTCACTTGTGAATGGAAGCAATGTTCATCGCTTCATAAAACTCACCTTCAGCTGTACCACCATCCCCAATCATCGTAACGGCTACGTTAGGGAGTTTCTTGTAGTGCAACATGTAACCAAGTCCAGCAGCATGAGAGTACTGTGCACCAATGGTAATGTTAATGGGCAGCGTTTTGTATTTAGCTTCGATCTTACTACCGTTTTCGTTACCGTTTCAGTAAAGTAAAAGCTGTTCTGGTTTAACACCACGGTACAACATTAACGCACCGGAACGGAAGGTAGGGCAGAACCAGTCATTTTCATTTAAACCCATGCCCATACCAACTTGCAGTGCTTCCTCACCTAAGTTAGGCGCAAAGTTCAACATCTTACCAGCACGTTGTCAAACAAGCATCTTCTTGTCCATGATTCGGGAAAGATTCATGAGGTAAAAAGCGTGCTTTAACTGTTCGTTGCTAAGTGTAATCTTGTGATTAGGGTCCATTAACTTACCCTCATTGTCATACACTTGGTAAAGTGTAGTAGGGACTTTGTTTTTAATCAAAATTGCCATAATTATTTGTTAGTTTAATCTTATTTATTTTTAGGGGTATAGTAACTAAAACCAAGCGCTTGCAACACGGCTGCTAACACAAAGTTGAACGGCTTATTGTAGTGGGGAAGAAAGTAAACGTCTACTAAACCCAGTTCACTGATCAACATTTTCTTCTGTACTGCTAAAGCAATATAGAAGATAATTTCACTGTGGTTGGTGTTTCACGAAAGTAATTGGGCACCCAACAAACGGAGCGTCTTCTTGTCGTAAATTAACTTAAAGCGCACCTTGTCAAAGGTCCCCATAAATTCGGGACGGTCGTTGTCATCAACAATGCTCACTCCCACATCAAAACCGTTCATTTTGGCACGTTTTTCGGTTAAACCAGTAGCTGCTAGATTTAAACCAAATACGTGCAGCGCATTGGTACCAACAATGGACTCTAACTTTACAGCCTTGCTACCAATCATGTGCATAGCAGCGACTAAACCACTTTTCACAGCGTTGGTAGCTAAGTCAATGTTTTCGTATTGTTCACTAGCTGCATCATAAATGGCAGCAGCCCCACCAATAACATACACATTTTCATGATTTAAGGCTTGGAGGTATTCATTAACCTTAATCGAACCGTTACGGTTAAACTCAAACTGCCTGTCTTTAGGGACAAACTGGGTGTTTGGGCGGAACCCAATCGATTGGATGACAAGGTCAGCATCCACCCGACCCTTATCGGTTTCAACACCTTTAACGACATTTTTGTCCGCATCAACAATAAAGCCCTTAACGGCACTACCCATCATTAGGTTAATGCCCGCTTTCTTCATGGCTTTTTCCAACTCGTTGGTAAACTCTTCATCAAAGTTATTGCCAGCTGGTTTGTCCAACATGTCAATCACAGTCACCTGTTTACCACACTGTCAAGCCGCTTCTGCTAACTCTAAACCAATGTAACCCGAGCCCACAATGGCTACTGATTTAATTGACTTGTCATGACGGAAGCTGTCAATTAAGGTAAGGGCGTGTTGGTACAACTTACAACTAATTAAGTTCTTAATATTGCCACAGTACTTATCAGTGTGGTTGAATTGCAATTGGGTGTGGGTTACTTCGTTTTCCACATTCATACAAATCGGTCAAGCTCCAGAGGCTACTACCAATTGATCATAGTGGTCCACAGTTTCCTTACCAGTGGCCAAGTCCTTCACGATTACTTGTTTTTTGTCTAGATCAAGACCAACTACATCATGAGCCATAAAGACATTAGCACCCATAGCTTTTAGCTCTTCAGGGGTGGAGTAGAACAGGTCTTCGGTGTTTTTAACTACACCACTAACTGCCAAGGCAATTCCACAACCCAAAAAGGAGATGTTGGTATTGCGATCATAAGCGTTGACCTGAAAGTCTTTGCTCTTGCTTAAAAGGGTTCTGATAAAACTGGTGCCGGCGTGATTGACACCGATAACAATCACTTTCTTCATATCGTAATCAATGTCTTAAGATTAATATTTTCACGCCAAACTAAGTTTAGTTAAGTGCGGTATTTATTTAATAGTGGAGCAAGATCTTGGTTGCCACATTTGGTTGTAGCTTTGCTTTACCGTGCAGTTTTTGCAGTTCAATTAAAAAACAATAACCCACAGTTTGACTACCTAAACGCGCTATTAGTTTGTCAATCGCTGCAACCGTACCAGCAGTAGCTAGTAAGTCATCAACAATCACACAGCGCTTGCAGTTTTCTAGTGCATCAACCCGCATTTCCAAGATTGAGTTTTGGCGGTATTCTAAATCATAGGTTTCACGCGCTAACTCACCTGATAGCTTATGGGGTTTGCGCACTAACACTAGGGGTAGTTTGGTTTTGGAAGCCAAAGCACCACCAAAGATAAAACCACGCGCTTCGGGACATACGATGCCATCAGCTTTAACTTCCTGGATAAACTGTGCCATTTGTTCCAGTACAAACTCAAACAATTCACTGTTTAAAAAAATTGGCGTAATGTCGTAAAAGAGGATTCCCGGAGTGGGAAAGTCATTAAATCGCTTAATAGCTCGATCAAGCGCTTGGAGTTTCTGTTTCACTGTTTATTATCTTAAAAACTGTTAAAAGCGTTTCTTAAAAGCTGTTAATTCCACTAATCAACTGTTCTTCAATTTGATCCACTTCCTTAAACATTTGCTTTTTGTGGCGTAAGGTAATATGGATTTGGACATAGTTGTACGGACTTAACCAACCATTGATTAAACCAAAGCTAAACAGCGGTAAGCCATTGACAAACACGCTAAACAAGGCCACAATCGCAATTAAGTAGAAGATCACTAGGTTAATCGTGCCGTTAATACCACCAAAGTTAAAGGCAGCGTACACAATGAAGAGTAGGATTAAAACACTGCTACCAATAAGATCTAACCTTCATTGGCTGTTAACATTGTCGTTAAAGAGGTTTTTCACAAAGACCCGGTCCTTCAAGCTGCGTTTAAACCAGTAACAGTAAGTCCAAATGGTAGTTTGACCAATGCGGGCTTGGAAGAACAGCCAGAACAGGTACACAAAGATAATTAGTTGAATTGTGGACTGGTCAATCCTAAAGATAAAGTTAGCGAGGTACATTGCCCCAAAAAGTCCAGCGGCTAACAGAATTAAAGCAATTAGGTAGCTAATCCCATAAGCGACACCATAGCGCGGTAGCGAATACAAGAGATAGAGCAAGCTAATCACCCCCACACCTAACATAATGCCAGTACTCGATTCGATGTCAGCGGCTAAAAAGTTACCTAACAGTTGTGGTGCGCCCAGATAAAGGCCGAGCATCACCACCCCAAAGGCGAGAACTGTCAACCAAATAAACAGCAAGCTCCACTTTTTCTTACCAAAGAAGTCGTGCTGGTTCTTACTGATAAAGAAGTCCGTTTGGATGTCGTTGTTTTCCGTTAAGATCGAGTAGTTACTCGATGTTTTGGTCAACGCTTGCGCGTCCTTTTTATAGAGGAAGAAGCGTCATTCTAACCCGGAATGGGATGACACAAACAACCACACTAAGATTACTCCTAAACCATAGTTTAAGAAGTAAGAGGTAATCGCTGAAACTGCCATTAAGGTACCTAACTGTTGTACCTGGTAGTTGGAGAGGTAAATCACGACTAAGGCACTCAATAACCAGCAAATGTGGGTTTCTATCATCGTGAAGAAACTACGTTTTAAACACAGTTTTCACGATTCCACAATTGAGGCGTTGTTCTTAATGTTACGCAAAAACAGTTCCGCCAGAGTGATCAGGTTAATAATGTTAACGGCAACCACAAAGAAGATGCCGACAAACGAAAAGACATCAACGACACCACCAACGGCACTAAAGAAGGCGAGCGAAGAGATGGCACTAAGCGCTAAGGCTAAGGCTTTGTAAAGGCCAAGTAGCTTGTAACGAATACCTAACGCAATTGTAGCGAGCAGGATAATTACACCCAAGGCAATGAAGCTCGAAGCAAAGTTAGAAACATCGCGTGCTAAAGTGTTGGAAAGCGGTGCATTAACTAACTTGGTGTTAGCAGCTGCAATGGTAGCGGTTTCGGGAAAACCATTGCTCAACAGCACCTTTACCCTACTAGCTTCCAAGTAAGAAGCAGGAACGTATGAACCATCATTGTTAACAAAGTTAGCTACTTGGAACTTAATGTTACCACCGTGTTCTAAGCGTTGGTTATTGCTATCCAAAAAGCTTTGTAAAGTGGCGTTTACCTTAACCGGATCACCATTCTTAGCTACCGGATCTTCTGTTCTAATTTCTGCCAAGATGTAATTGGAGAATAAACCAGAAAAACCAGTAGCATCACCGCTGCGCAGTTTTCTAGTGTCCGCGCTGTTAGCTACAGCGACAACATCAGCAGCGTCCTTATTGTTATTGTCTTTGGTTGCAACTTGTGAGAAATGTGTCGGTGCTGCATAGATGTAATAGATATTTTTTAAGGTAATGTCACTAGCTTTGGTAATTGGTGAATTACCATTATTATTTTTTTGCAAAAACAAATTATCACCAGAGGAAAAGTTACCCTCAGCGTGCAAGAAGTCCCAGAGGTTCTTTTCACGTTCGTTAAAGGTGAGGTAATCAGTACCACCTTTAGGAACGTTAAAGATGTTGCGCACATACTGTACTGCACCTTCCTTATCCTTCCATAAAACTAAAGTTTTATTTCCGTTTTGTGCACTACCGTTAGCCTTGTCTTTAAATCATTTACCGCTACCTTCAACACTCTTTTGACCATTACCAGTATTATAGTCAGAATCAGTTAAACTCTTACTCTTAATAAAGTCCAAAAAGTCGTTGTGTAAAGATTCTTTGGTAATAACATTTTTGCTGAGCTTGTACTCAAACGTTAAGGCACTGTTGGTGTTTGTGGTTTGGGGAACAACTGCTGTAAGCGAATTTCTAATAAATTCCTTATCAAACGAGTCAAAAAGAACAATTCCGTCAGTGGTTTCTAACGAAAGTTTGTAGTTGTGTTCAATTGCACTTAAAGCGTTGCGCTTGACCTGTGCATCTATTTGCCGCTTTCGTGCTCGGGTACGCGCCTCTTCACGACGTTGCTCAAAGGTAACAGAAATAGAGGGTAAACCATCATTAACTAGTCAAGAACTGTCAAAAACTCCTTTATCTGAAAGTTCTTTTGCACTTGTATCGTGTGAGTTTTTCGTGATGTTAACAGACGAAAAACCCTGGGCGTAGAGGCTGTTGGCGTAACTCTTTTCAATATTGTTGAGAAAACCGTTCACATCGGTAATCATTTCAGTATTGTTACCGCTGCCATTGCCGTCCTTTTTCTGGGTAGGATCTAACTCCTTGTTAGCGTCCTTGGACTTATGGTTTAAAAAATAAACAGTGGTGGTAGTGGAACCGTTGAACACTACCCCTAAGCGACTGTCGTTTAGAAGCTTATAACTACCAAAAATAATTCCAAACAGGCCCAAAAAAGCTAAGACCAGGATAATTCCAATCTTTAAGAGCCAGTCAAAGGAAACCCGCTTTTTCAGTCCCACTGGATGTTTTACAATTGACTTAAATTATATAAATTTAGCAAAATGGCCACTTTAGTTGAAATTGAACGCGATTTTTTACAAAAAACAGCCCAAAAATTTGCACCTGAAGTGGTTGCGTTGATTACTAAAGCACTCGATTACTCCAAAAAGTGACACGGTGAGCAAAAAAGGCTGAGTGGTGAACCGTTTTTTATCCATCCCTTGAGAACAGCACTGCGCTTAGTGGAGTGAAACATGGACAGCAACACCGTGTGTGCTGGTCTGTTACACGACATTATTGAAGATACCCAGGTCACTGAAGCTGATTTAACTGCCATTTTTGGTAAAGAAATTACTGATTTAGTGGTAAAAGTTACCAAAATTACCAGTGAATCCAAAAAACAACGCCAGCTAAACCGAAAGAAAGAAGATTTAAACCTCAAAAGTTTGGTTAACATTGCGATGAGCTCGCAGCAAGAGGTCAATGCTTTGGTGTTAAAGTTGGCCGACCGACTCGACAACATCTCCTCAATTGAATTTTTAGCAGTCGAAAAACAAAAGATTATTGCTAAAGAAACACTCGAACTGTATGCCAAGATAGCCGGTCGAATTGGAATGTATCCCGTTAAAACTCAACTAGCCGATCTTTCCTTTAAGGTATTAGATCCAAAAAACTTTAACAATACGCTGTCCAAGATTAACCAGCAAAAGGTCTTTTATGACAACGAATGGGGCAACTTTAAAAAACAGTTGGAAGAGATGCTGGAACAAAACCAGATTGAATACCGCTTAGAAAGTCGAATTAAAGGCATTTATTCTACTTATCAAAAATTAACCTTTCACGAGCAGAACATTGCCAAAATCCACGATCTGTTCGCCATTCGTTTAATTGTGAAGTCAGAGTTGGATTGTTACCACTTACTTGGTTTAATCCACCTCAACTTTACCGTGTTAATGAAACACTTTAAGGATTACATAGCATCCCCCAAACAAAACTTTTACCAATCAATCCACACGACTGTGCGCTTGAAGGGTTTAAATGTGGAAATCCAGATTAGAACCCAACGAATGGATCATGTGAGCAAGTACGGTTTTGCTAGCCACTGGATTTACAAAGAGAAAAAGGAAGGGTTATTGGCAAGTGCTTTACAAGTAAATTACTTAAACAGTAAACAAATGCATTCGCGCGATTTTTTTAAACGAATCTTTGGTACTGATATTATTAAGGTTAATGTAAGTTCCGATAACGAACCTAACATAGTCAAAAAACTCAATGTTGAAAGTAACAGCAAGTTGCTCGATATTGCTTATGAGTTGTATCCCAAACAGTTCAATAAATTAGAAAAAATCAAACTCGATGGGGTTGAGGTTATGTCGTTTGACGTAACTGCCGAAAACGAAATGGTAATTGAGTTTTGTTTTGGTAAAACCAATAACCTCAAACGGCGTTGGTTACGTTATATGAATAACCCCGTATTTCGCGAACGGGTTAAAAAGGATTTAAACAAGTTAAAGAAAGCGGTTAAGTACAGTGAACTACCTCTATACGAAAAGGCCTTGGAAGAGTTGCACTTAAAGCTAGCTGATGAAACCCAGATTAAACAACGCTTAAATGCTTTAGGGATCAAAAAACTGACAGAGTTCTTGGAATTAATCGAATACCCCCACTTCCCTAAAAATGAGCATTTGTACTTTTTAGCTAGTAACAATCAGAAGTGAAGGGAATTGATTAAACCCATTAAGTTCGCTTTATCACAAGCTGTGTTCCAAAACTCTTACTTTGAACAAATTGAGGGAATTTACATCACCAAAATTGTGATTGAAACCTGTTGCACAAAGATTCCTGACATGCCAGAACAAGTTATTGGCATCTTAATGAAAAACATTCTCAGGGTACACCTTCATGATTGTAGGGAATTAGCTAATCAAAAGCAACCCAAAATTATTCCACTTTATTGAAATGCACACCAATTAAAGATGCGACCACGTAAATTCCGCTGTCAAATTAACATTCGCGGTGTATGGAGTGAAACAACGGTCAATAAAATTGTTCAAACCATTATTGAAGGTGATAGCTATTTAGAACGAATTATTCCCAAGATCGATAAACAAAAAGATGAGTTTGAGCTCAACATTACTATGTTTATCGACAACTATCACCAACTAATCACAATTATGGAGCAGATTACTACTAAGAATATTAGCTACGTTTGAAAGTACCTTTAACTTTCAAATACTTCGTCATAAACATCTTGGTTCTTTTGTAAAAGTAACACTAAGTGTTTCTTCAATAGCCTTTGTGGGTTAAAGCGCGATACTCGTACCCCATAAACGCGTTTGGTGAAAATAAACAATAAGGCACCAGAAACAACAAATGTACCAATAATAGATACCCCGACATAAAAGACAATTTGTGAAATATTAGTTACGTATTCCTTGAACTGATCATTTTCGTTAGGATTTGGAAAACGTTTTAAGATTTCGTCCACGTATTGATTAATCTTTTCAATTGTGTTCTTAACTTCAGTAAAAGTAGATCCTTTTGAGAAGTTGTTAACCTTGCTTAATGAATCTTCGAGTTGGTTAAAACTGCTTTCTAAAGCACTGTCACCCAGTAATTTTGTCAATTCTTTTCCTTCTTTAATTGAACTTTCAACATCACCTAACGTTTTTACAAGTTCGTTACTAGAAAGTGACTTTGAAACTTTTTTAATTTCCTCGTTATATTGTTTAACCTTGTTATAACCTTCCTTGTAATAGTTATTAATAGGTTCACGGTTTTCAAAGGCAAAGTAACCCGCTGCTGTAATGCCACCAACAAGTAGAATACCAATCAAAACAATTAGGAAAGTGGAAAGCTTTTTTAAAAACTTAAACATTATTGATCAAGCTCCTCTAAAACAGCATCTTCCAACTCTGGATAACGATCTAAGATGTCATTAACATGATCAGCAATTTGATCAGTACTGGTTAAACGCGAAAACGTTCTAAGTCCTAACTTTTTCGAACCTAAGAAGGTCATTACAGTAGAGACTATTAGCACTGCTAAAACGCCACCAGAAACACTAACTAAGGTAATTGCGCCTATGGTGTAATATTCATTAAACTGTTTTTCTGTTGGAATTTTGTTTTTAATTTCTTTGTACTGTTTAATGATGTCATTTGCTTGTGTAGAGAACTCTTGAATATCTTTCAAAGCACTATTGGTTTTACCGTTAGTATCTCTGGTACTTACACTAATTGTTGAACCAGAAATCTTAGTAATTGGAATAGTCGAAGAAGATGTGCCATTTTGACCAGTTTTACCATTATTAGCGGTTTTTAAAGTATTTGCATACTTTTCTATTTGACTCTTAGTAGCTTGTAGACTCTTCTTAGCTTCTTGTAACTCCTTTAGTTTTTCCGGACTCACAAGATAGCCGTTTAAGCCTTTAGCTTGGTTTATTTGCTTTTCGTACTCCTCTAACTTTTTAGTTGATTCTTCAACTTTCTTCTTAACCTCTTCTATTTGTTTATTAATGGATTCAACACTAAACTTACTGGAACTTCCATTATAAGTATCTATAAACGCTTGAGAAGAGTCAAAGGAACTGTCGATCGATTTGCGGGTATCAATGTAAAGTTTAGCTGCGTTGTCTTTAAAGTAAAGTGCAGCACCGCCAAGACCTAACAAAACCAAAAAGACAAGCATCCCGAAGAGCAGAATGATTGTTTTGGCAAAGCGATTAATAAAAAGGAGCATTTAAAAGCTTAAGTTAGGCTTAAGGTTAATATATTTAACCAAACGGACAACCCGCTAAAGGTTAAGTTCGAATCTCTTGGAAAACATTTTCCCGATCCAATTTTTCTTTCAATTCCGCTACAACGGTTTCATCTTTAGAAACATTAGTAATATTGAGAATTTCGGCGTTATCACGTAATACACCGATTAAATCAGTAACTGATTTGCTCATATCAGTGACACTACCAATAACTGGTTGGAACTTATCGGTACTCTTTAATGCCACTTGTTCTTGTGGACTTTCAAACTTCACGGTGTACTTTAAACCCGGACGGAGGTTTTTCACTCCATATTCAAAGACAACCCCAATAAGTTCAGCGCGTTGTACAATTTCCTTTTGGAACTCACTGTCACGGGTAGCGCGTTCCGCTTCGTAAGCACTGGCACCCGGATAGTAATTTTCGTTCACCCGGTACATCGTGTTGCCGCCATGAGTGTAAGTTTCCTCTAAGTGGAGATTAATGTCTTTTAGAGCAGCGTAAACGGTGTCGTTGTCTTTATTGACATTTTTTTCATTAGTGTTTTTCACCAAATAACGGTCAATGTAACCACCAGCTCAGTAACCTTGCATTTGTCGTTCAAAACGACGACGAATGTAAATGTCACTAACAGCACGCTTTAACTTAGATGCATCAATATTGTCTTGGTTATCTTTAAGTTCTAAACTAGTTAAATCAATGTAATTAAACGGCTTGGAAGCAAAAAGGTCATAGATAATATTAGTGTGATCGCCTAAAACTAGTGGATTAAAACCAAAGTTATTGTCTTGGTTAGTAGAGTAAAACTCCAACTCTAACACGTTTTTAAAAATTTGCTTAGCAACATTAATCGAGTGGTAGTCACCATAAATTGACACCTTTTTAACGCTTTCTGGTAATTCCAAATCCTTTAAGGTAGAACCATCACCAGTTAAACCATTAATGATGACGTAATCAGGATTACGTTGTTTTAAATTGAGGTTAATGCCGTCAGTATTACCTTGCTTAATCCTTGGAGCATCAAAGGCAATCGTTTTTATTTGCTTAAAGTGATGGTTTGGGTAAACGTTGTATAACGGTGAACCGTTAGCAACATAGTAATTCCACTCTTTAAACAAGTACTCGCTTTGTCCATTGGAGCCAGCATAGTAAGCCTTATCCACGTCATGGAACCAACCAGGGAACTTAGGGACACCTTGATCGACAAAGATATCATCGTAAGCCCTATTGTTAGCAGTTAAAGTGCCGTTGGTAGTTTGCAAAATTGGCATTACTACCCGGTTGTTAAAGTATTCGGTTGATTGGTAATATTTGAGCGTTTTCCCGTCCGCTGCTACTTGGTCGTAGTAACCCGCTTTAAAGTTTGGCGTGATAAAGGTAGAACTGGTTTTAATCAAACCGCTCACCTCTACCTTAGGCACTGGTCGAAGTAAGTCTTTTGGTGCATCAGTAATTGCTTCTGATAAGTTATTGGCAAAATTACGGAAATCCTCACGGAAGTTGGAACCATCACCAAGATCGATTTCACTGCGGTATCCCCCATCGGTTAACGAAATCGAATTAAGTTTAGACTTGGCGTTTTGGTTTACCTTGGAATAAAGCTGGAAACTCAGGTTACCACTGCGCCCCGAAGCAATGAGTGTTTCACCACTTTGTAGCCTTAATACCTCATTAACGATTAGAACTGCGGAAAAAGCATTTATTGCAGCACCAAAGAGACCAAGCGCTCAGAAACCACCCTTTTTGAGGCGCTTTAGCGTCTTTTTGTCCTTGATTTTGAAATTTAATTTCATCTTTTTTAATAGTTTAAAATTGCAGAACTAAAACAGTTCTTACTCAACTAAATACTTGAACTACCTCAACGACAACGGGAAATTTAATTGAACACCTATTAAAGATATAGTGTTCTTCTCTATTTTAGATTTAGTAATCCCTAAAGGAGAAAATTTACGCCAAATGTGTACCAAAAAAAGAGAAATTTTTGGCGATTACGTTATTCATTTCTTTCCAAAATAGAGGTTAAGCGATAATAAAAGCACCCGCTAAGCAGGTGCTAACCTTCTTTTGGAAATGTTTAAACTGGAGCGAGTAATCGGAATCGAACCGACACGATCAGCTTGGAAGACTGATGTTCTACCACTAAACTATACTCGCAGAACTTGGTGCCCAAAGCCGGACTTGAACCGGCACGCTATTGCTAGCGTGGGATCTTAAATCCCATGCGTCTACCATTCCGCCACTTGGGCTGGTGACTCACCCGAGGATCGAACTCGGGACCCTTTGATTAAAAGTCAAATGCTCTACCGCTGAGCTAGTGAGTCAATAAACAGGGTTGCCCCTGTCATTTTATGAATGCTTTTTTACTGGCTGGGATGGATGGGATCGAACCATCGCATGATAGAGTCAAAGTCTATTGCCTTACCGCTTGGCTACATCCCAATAAATGGTGGACAGGGAGAGATTCGAACTCCCGAAACCATGAAGGTGTCTGATCTACAGTCAGAAGCGTTTAGCCACTTCGCTACCTGTCCAATAACCAGCAAAAGATATTTTACAAAAATTATCAGTTAATGATCTTAACAATTTTGACCCGGTAGGGCACTTCTATGCCAACTACGTCACATTCATCATCCACTAAACGACCATAAATGGCGTGTGCCAAAGGGGATTCATTGGAGATGCGGTGTTCTTCGGGATTAGCCTCTAAAGCTCCCACAATGGTGTACTTTTCGTGTGTTTTCGAACTGTAATCGTAAATTTCGACCGTACTACCCAGGGTAACCTTATTAGCCTTTTTTGTTTGCGACTCATTAATTAACTTAACGTTGGCTAAAATGTCTTGGATTTCAGCAATGCGGGTTTCAATTTCACCCTGCTGTGCTTTTGCTGCATCGTAATCAGCGTTTTCACTCAAGTCACCCTGATCACGGGCTTCTTGCAATAACTTAATAATAGCGGGACGTTTTACTTGAATGAGGTGTTCCAGCTCCGCTTCTAATTGTTTTAGCCCCTCTTCGGTAAGGTAATTTTTGTTAAGTTCCATTAATTAGAAATTGTGTTTAAAGTTAAATTAAATCTACTTTTTTTTCCCAATATTTGCCAAAAAAATGCAAAATTTTATTTGCAATTTGACCTAACAGCACTAGTTAAAGCTACTGCTTGTTGGATGACAGATGACAACGAAAGACAATTCTGGCATTAAAAGTAAAAGACGGATTCGTTTTTTAGCTTTAGGAATTATTATTTTGATTCTTGTAATTGCTGTAATCACCATTTTATTTGTCACGGGTGTAATTACCCGCGCTAACCAATAAAACGTAGTTGATGGCCAATAAAGATCAAAATCTAACGCTTTGATACGACCAACTACTGAGCAAAGCACAATTAGTGAGCTATGGCGATGTGAAGGGCACTAACTGTTTTTTACCCAACAGTTGGAACCTCTGACTACAAATTCAAAGACTCTATAATAACGCTACTGCTCTAATTAAATTAAAGGACAAGGTTATTTTAAAGCAGTTTATCCCGATTGAACCACTGCCATACACAGTGGAGCAAGTTCAACTACCGACCCTTTCCTTTTACAGTGAGTACCAAAAGGAAAAGCGTCATGTGGAGGGCTTTAATCCTGAGCTTTTTTTAATAGAGCAAATAGGTACTAAAAAACTCCATGATCCGCTAGTACTACGTCCTACTAGTGAAATTGCTTTTTGTAACTTATGGAAAAAACAGAGCTTTTCTTACCAAAACCTTCCCGTTATCTATAACCAGTGAACCTGTGTTTTTCGCGCTGAAAAAAACACCCGCCCTTTCTTGCGCAACAGTGAGTTTTACTGACAAGAAACCCACGGTTTGTTTAGTGATGGAGTTAATTCTGAAAGCGCAGCAATTGCTTTTTGAAAACTGTACCAGGACATTATTGTCAACCAGCTCTGTATCCCCGCTTTTGTGGGGTTAAAGAGTCCCAATGAGCGCTTTGCCGGAGCACAAAATACCTGAACGGTGGAAAGCATTATGCCCGATGGGCAGGCATTGCAGTGTGCCACGAGCCATGATTTAGGGCAAACCTTTACCAAACCGTTTGGGCTAACCTTTCAAAACCAAGCCAACCAACAGGCCATTCCCTACAGCTTTAGTTGTGGAATTTCTACAAGAATCCTCGGTGCTTTACTGTTAACCCACAGTGATGACTTTGGACTGGTACTACCATGAAAAGTAGCACCAATTCAAGTCAAACTGTACTTATTTGACAAAAAAGGTGATACAAAGACAGTAGAGTTAGCACAGAAAGTGCAAACGTTGTTGGAACAATTAGCAATCCGTTTTCAATTTATTAAGGTCGAAAACCAGCTCGGTAAACAGTTAGGACAGGGTGAAGTCAACGGCATTCCCTTTCAACTAATAGTTGACAATCCCCAAACAGTCAACATCTTTAACCGGTTAACCAGGGTCAAAACAGCTTACAGTTTCGAACAGTTGGCAAGCCGATTTGTGGAGCTCGTGCAACAGTACCATCAAGCAATGTATGACAAAGCCAAAGCAGTGGTACAGCAAAAGGTGGTGCAAGCCACTACTTTAAAGCAAATTGAACAAGCCTTTAATGACAAAAAGGCTGTTTTGTGTGCGGTTCGTTTAACCGATACATTAGAACAGCAATTAAAGGAACGGTACCAGGTCACGGTGCGCTGTTGCTTAGAGCAGCTTCAAAAGCCCCAAATTTGTCCGTTTAGCGGTGAGTCAGCACAGGACTATGTCCTGATTGCGCGTGCCTATTAATAACTTTTTACGAAAACTTTTTGCTTAAAAGCAAACCCTAAATGTAAAGGCTAAAAAGGTGGTTTTTATTTTCCACTTCTGGTCGTTTTTTGTCAATAAAATCAGTTAAACCTTTAAATTTTTTAAGGTTTTTTTGATCTATTTATGAGTTTTAAAGTTAAAAACCAGAGTAAGCACCTATACTCTTTAATGACGAAATTTAAACGTTCGCAACTGATCTTGAAGCACCAAAGCAATAACTTTGCCTCAGAGTTGTGGAACGAAGAGGACATTATCCGCTCCAAGCAGTTTATCGAACTTATAGAAGACACGCTGCTCCACCTCAAGAAGGATACGGTCGACTTTATTTACGACATCTTCATTTATGGGAAGAAGCCGTGTGATATTAGTTACTCCAATTCGACCTATTACAAAAAGTTAAACAAGGCTGCAAATAGTTTTTTTGACCATTTTGTCTGGGAGGCTCCAATTTATAAGACAAAGGAGCTTAAAAATGACAATTCTCACAGTTAGACAAATTCAAAATAATTACTATACGGAATACTACATGGGTCCGGTTAGAAACTTTGCTGACAAAAAGGAAGTTTACTTTGATGCTAAGGTAAACAATATTGAAAGCAAAGTCAACAAGGATAAAGCCTTGTTACACATAACCCTGGCGTTAAAGTACGACAGTAACTTTCCCAGTAATATGTTCCAAGCTCAGTTTAAACTGGGTAATTGAAGTAGTGAAAAGATTCCGCTGCAAAAAGCACCGGATAAAAAATCCGATCTGGAAAATAAGATTCACTATTTTTATGCCACTTTGGAAGTGCCACGAACTGCCCTTATTAAACGGGAAATTAACCGCATTAGTGAGTATATTCGTGAAGAATTAACAATTGCTTTTCGCTTAAATGACAGTAGTGATCGCTACCATTGGAGTAACTATAACCTGTTCGATACAGTGGCAGCTGATATGTACCAAACGGTCATTAAAGAAACCGTTACCTTTGGTAACATGATTAGACTAAACCAACTCGAGGGCGAGAAGGAAGGTAACATCCAACAAAGTGAACTTAAATATGGTTGAACGATGTTGGACTACCGCAATATGGGGCCGCTGGAAGAGGTTAATAATTTAATTAATATTAAGTTTAACCAACCAGTAAAAGTGGTAAGTGTTGGTGTGACCGTAAGCTATACAGCGCCTGATGGTAAACGCCAAGAGCTCAAAGATCAAGCTGAATACCAGCACACCCTCAACCCCAATGAAGAGTTCAAACTGAACTTTCCCAGACGGCTAAGCTTTAACAGGGTAACTAAAAAGTTGGACTTTGACCCCAATGGTAGTGCCGTGTTCCTACCACAGGGTGGCTTTGGTAGCTATGAGATCAAACTGGAGGCTAATGTCGGCAACCAGTTCTACACAATTGTGGCTACTAACAGCTTTGAGTACGCCCATCCGTTTGACGATCCCAAGACCAATGACTTCTTTCTGGTGCAGTACGCTCCAGTCTACAGTCTGTTTAACTTTAGCGATTTAATTCAGTAATTGACCATGATCTTCTCAATTAGTAAACGCAAGTTAATTTGTGGCTTTTCCCTAGTAGCCCTTACAATCGCTGGGATTGTTGGTGGGGTTTACTTAGTCACCAAAAACAATCAACAAACCACACCACAAACAAATCACTTTAATGTAGCTGACCCAGTCAACAAGGTCCCTAATTGGCGCAAACTAGGTCCAGAAACACAACGCGAACTGCGCGATCTTCTGTATCCCTTAGATGACACAGGTTACTTTATTTACAAGTACGGTGCCATTAGTCGCTACCTCCAAAGCCAAAAGGAACTGGATGAACTAGTCGATTACCGCACCGTGTTACCCTCCACCCAAAAGCACTTTAAGTATGATTCCTTTAACCAAAGCGTGTTGGAAAGTAAGCTGCGTAAGTGGTTAATGAAAGCCATTAAACAACATCCGTACTTTCAGCACTTTGAGTTTGATCCGGTGCTTAAAGCACAGTACAATATCAACATTCCTGCTCAAAAAATTACGGTGAATGCAGTTTGGTTTTACAAAAAGGATAATGATCTCACCACGGGCAAGCCAATCCGCTATTGGGATCAGTTTGAAATTAAACTAAAGCAGTAGTAATAAATCTTTATAATTCTTATTGGGTTTACCGATGCAAGAGCGTGACATTCTTTTAAAAATTAAAGAGATCGCTAAGGCTAAAAACTTCAAAACTGAGTTAGATGAAAGTGTGTTGCAAAAACCCTTTCGCGAACTGAAAATTGACTCACTTGATATGTTTAGCGTGATTGTCAGCTTAGAAAAGGAGTTTGACATTATGTTTGAAGATGAAAAACTGATGCAGCTCAATAATCTCGCTGAGTTAATTGCTGAGGTAAAACACCTAATAAGTCAAAAAGGGGTATAGTTCAAAGGTAGAACATCTGTCTTCAAAATAGAGTGTTGTGGGTTCGAGTCCTGCTACCCCTGCCATTAAAGATTTGGCCTAGTGTTGAATGACACTGGGCTTTTTTATTGCTTTGATTCAATCAATTCAAAGAAGTTGGCGTTTTCCTGATCACGGAAGGTGGTTTTCACGCGACGAATTTGCTTTACGTCAATGTTCCAGTGCTTACATTTTAATCATGGTGCAAAATCAGTTTTGTGTGTTTCTCACTGACTTCAGTTGTTTAAAACCACGGTAACTTGCTCGTTGTTGTTAGTGTTAGTAAAGGTGATGAAAACAGGTTCAAAGCCTTGTTTGTTAACCTTTAGTTTGGGTGGTACAGACTGCTTAATGTCGAGCACACCGGTTTGATCATCCACCGTTACTTCGGGCAACTCTACCACTGTTGCTGGCTTTTTGAGATAACCAAAACTCAAATAGCCCAAACTAGCAAAGGCTGAATTTTGTCAATCAAACCCAACAGTTGTTAAGATATTGCGCATGTATTCCACATTACCGTTCACTGAAAGGTGGGAAAATTTATCGGCAAAGTAAAACGACTTCTTTAAAAAATCAGTGTTAAACAGTTTGTTTTCATTAATATCAATCCCACTCACTTTAGCTAACTGTAAGAACAAACTCTTTTGGTCTAGTAAATGAACAAAGCTGCTGTAATTACTATCTTTAGGAATACCTTCGAGAAAATCGTTAAAGGTTTTTCAGTTCTTTTCAGAGCTTCGCAGTAAGCTAGATGGAGCAATGTTGGCTAAAACAACATGGGCTTGAGGACTTTGCGTATGTAACAGATCCAGTATTTGGGTAAGATTATGTTTGTATTGCTCGACTGTGTAAACCCCTTGCGCTAACTGGTTAATGTCATTAGTACCCAAATTAAGTACATAAACATCAATGTTTTTGTACTGCTTAAAGCGATGATTTTGCATGTACAACTCTAAGGAGGTGTTACTACCATTAATGGCACTGTTGATCACTACATCACGGTAACGACCAAAGTCATTTTTCAAGCTTTGTTCCAACAGTTGTGGGGCACTACTGTATCCGTCAGTACCAACACCATGAGTCATCGAATCACCAATAAACAACCAGTTGGCTTGGGTTTTATCTTTTAAGAAATCAAAGATAGTTGGTGTGTTGTCTTTTTCTTTATTAATCCAACCGTTAATTACCTGGTATGAACCTTGCCCGTCTGTCCTTGTAATTTGAAGTAGGTAGTTGAGTGGCTCATTTTCTTTTATATCAAGTGCTTTATGGATATATTTCCAGTAAAGAGATATGTCAATTTGTTTGTTAGTAATGGTACCGTTTCAACGCAAATAGCTGGTAGGACGTTTCTTAATATCTCTAGCGTTAAAGTCACTCGCAAACGAAAGCTGGTACTGCAGCCCATTAGGATTAACCTCTGTACTTTCAATTTCCAACTCTTTGGTTTTAGTCTTTCAAGTAACAGTAAAGGGATTGGAAGTAGTTACCAAATTAGCTTTAGCATTATTGAAAAAGTCCGTTTCTAAGTTGTAGTTGGGATCATCAAAATCAATCCTGTCATTAATGACATTGGTGTCATGGTAATGGCCAAAGCTGTAGTAATTTTTTACATCAAACTGGGTTTCTTCATTAATTGGTATAGCTTCTAGTGATGGTTCTGTAACTTCCACTGGCAATGGTTCAGCGCCAGTAGCTTTTAACACTTGTGCTAACTGTTTACCAATCGCAAATTCTCCTTGCAAACTTAAGCTAACTTTGTCTTCACTCAAATAATTGCCGCTAAGTCAATCACTAGGTACAAAGTCACCTTCAAAGTGATCAACTACCTTAATTTTGGACGCAAAATTACGGTTTTGATCAGTTAATTGCTTAACGGTGGCTAAAGCGACAAAGTTAAGGACACTTAACTTCTCGTTAAAAGCTGAATCATTTGTTTTTCAGTGCAATTGCAAAATGAAAAAGTTGTCCTTTTCAGCCTTTTCACCAACTTGCTTTTTAATGAGTGCGGTTAGATTATTGACAAACTCCTCGTAATAATCAACCCGCTCTGCATCCGCTTGGGAAGTGAGGTAAACTAGCGTACGTGGTTTGTAAATTGAAAGTACCCGTTCTTGAAAGCTCTCATTAATATCTTTCATAGTCTGCCCGGCAAAACCAAAGTTTGCTATCTTACCTTCAAACCGAGCACTAGCAAACACAAACTGATCGCTAAAATTAATGTCGCTGATAGTTTGTTTATCACTGTTTCATTCGCATAAAACATCAACCCGATCAACTTGTCCAGCTGGTCAGTTCGGGTTGGATCAAAAGCGCCAGTACTGGTCAAAGTAAGCGACAAAGTTACGCGCACCGTGTAGTCGAGCAAAGTCGCTAACTAATTCCTTACCACCAGTTCAAGCAAAGCTTAAGGATGGGAGTTGGTACCTAATTTTGTTGTCATGTTCTTCACCATCCCTAGCATAAAAGTCCAATACCTTGCTTTGGAATTCACTACCCCAACTCGCTATTTCTGTTTTGCTAATGTTAGTTGGTAAATTTAAGGGTTCAATCTTTTGTTGATGGGTGGAGCTCGGTAGTTGGATGTTGATATTTTTCACAAAGAGTGGTGAAACATCACTCTGAGTGCCCATTAAAAAGAGGGTAAAGAAGGCACCAACACTTACACCACAACCAAAAAAGGCTAGAGAATTGAGTCAGTATCTGTACTTTGTGCGTTTTTTCATATTGGTTTATTCCGGTTCATTAATGAGTTATTTTTATTGAATAACAGCAACTGCAACTTTACCTGTTATTTAGCTATAACTAATTTTAAAACTTTGTAATTTTGAACCACATTTTTTTGAAAGAATTGGCAATCGGGATAAATTGCTATTTTGAAAAATTCAAAATTATTAAAATTAATCCCAAATGTTTTTTGGGTTTTCAAAAACTCTCTAATAACAAATAAACAATGGTTATTAAGAAGGGTTTTTTCGCGTTATCAAGTTGCACATTAGGTCTAGGTCTTATTTTAACTGCATGTGGTGCTAGGGGTAAATTTGACCAAGTTGACGATGGTGTTATTAAGCTAGCTACTTCAATCCAAAATAAGGACAGTATCGCTGCTTTAAACACCATTTACAAGAAGTATAAGGAACGCCATCCAGGGAGTTATCCAGTTCAAAATTTTCAGGTTCCCGGTGGTTACAGTGGTTTGCGGAACGACATTAGAACGCGTTTATCAGCTAAAGATGGCAACAACTTTTACAACATAGTACTCAACTATCCTGATGTTGTCTCCTCATTGGCTACAAGTGACATGCAACTAATATTGGATAACGTTGACACCAAGCTCCTTTCGAAAAATTTCTTGAGCTTTAACGAGCGAATTGGTGGTGTACGACAAAAGGGGATTTATGCAATTCCCATTTCCCTTTCCACTGAATTAATGGTGCTCAACGGACCGGTATTGCACTATATTTTGAACAGTGCTAAGAAGAAGGAAAACGGTGCTCAAATGATTAAAAGATCAGGTAGTTTTTCCACTGTTCAAAAAGGTACCATGGCAATCGACATGAATGATCAAAAGACCAAAGATCTTTGACAAAAAATCGAAAACGCTGCTAAGGCTAATGGTAAGACAACTAGCACTCAAACAAGTCCACAACCAAAAAACGCTGTTAGCTCACTCCAGCTAAAACAGGCAGCTGAAGGTACTAGCACTGATAATTCTCAGGATGCAGAAAATAGCGATAACGAAATCAAAAAGACTTGGGGTGAATACAAGGAAGAGGGTAACCACACACTGAAGGGTTACACCTTTAAGGCTAGTGTCTTTGAAAACTGAAATGAACTATTAGACTTTTCCACGCGGGTAGCCAATTCGTTCCCAGACAAAATTAAAAATCAAGCATCTAAGAAAGCTACCGAACTGCAAGGTGTCTTTGGGGTTGACAGCGTCTCTGGTGCTTTATTTTCCGCTACCTTTGCTGCTGGTGGTGGAGACTATGATAAGTTCTTCTTTAATGTAAAGAATGGTCGGGGTAATTTTAGAAATTTACTCGAAAAGGGTAGTTCTTACAATAATCTGCAAAAGGTCTTTAACGATTACAAGCAGTTAATTAGCTCTAACGGTTTGTACATTAATAAAGGTGGTGCTTATTCCAGTAACTTCTTAAAGTTTCACCAGTTAGCTTTTAGTGTGGGTTCCAGTTCTGGTTACCATTTTGCTTTTGCTGGTGAAAGCGCTAAACGCTTGGAATTTGGCCAGAAAGCAATCGAGTACCCACGTGACACTTACGAAATTAAGGCACCTACAAATAGCCAAAATGGTAATGGTACCCTTCTAGGTAGTTTTACTAAAAGTAAAAGTAATGGCAAAGAACAAAGTGGGCAAGATGAGGATAACCAGACCAGTGAAACGATTGAGCTTTACAAAAGCAGCGTTCCTAGTGGTAAAGAGGCGGGTAAAAATGCGCTTGCTATTACCAATCAACAACTAATTTCGGCTTTAGAAAATGCTGCAAAAGATAATAAAACCTCCCAACCACAAGCACGATCACTTACAGCTTCTGATCAAGTTCAAATTACACAAAGTTCAGATAAGGTTATTGGTTACATCACCACATCTAATTTGGACATTGACAACAACAATACCTTTGATGTTGGTAAGTTAAACGGTGATAAGTCAACCAGCAAAATCATTGTTAACGCTACTTTAAAAACCTTAAACAAGATCAACACCTTGCAAAGCGAAGAGGGCATTATTCTGCCCCATCCACAAAAATATAAGAGTACTGATCCACAAGCTGTCGCTACCGTTCAGGGTCCAAGTATTATTGGTGTTCATGCTAACGCTAAGGAAAACGCTGAAACCCAAAAATTTATCAACTGGTTTATTAACCAAAAAGAAACTTGACCTGAAAATAGTAAGGGAAATAAGAATGGCCAAAATGGTCAAATGACCGCAGCACAATACTTTGCTAAGAGCTCTGGTTATGTTTTACCTTACAGTGAAACGTTTACTAAACAAAGTGAAGACGAACACAGCACAACTAAAGACGCTTATAAGATCTTGAAAGATGTAAACGATGGTAAGTTAGTTGGTTACAGTGATCCAAGTGATTTCCGTTCTGGTAAATTCCGTGACACAATTGTCGCTGCTTTTAGTGGTGCTGTAAGCTCTAAAGCTGACTTTAATAAGTTCTTTAAAGGTTTTGAACAACAATTAGGTCAAGAATACAGAAGGGGTTAATCCCACTTCTCCCCCACCCTTTCCCGGTTCTCCACCGGGTTCAACCTCGCGGGGGCACTCCTTGACCAGGCGTTGGATTACGTGCCCTGGATTGGGAATGGGTACAGGTATGGGAAGAACCACCGGGGCGTGGATGATATAACCGCGCCTAAAACCGGCGCGGGGTCGTCCAGCGGAACTAGTACGAACACAAGTGGTTCGCGTTCCTTTCTCCCGACGTTCTCGAATGTCGGCGTTGGCCTCAAAGCCAACGTCCAAGCCACGCTTGGTGGCAGTCAGACGACGACTACAGGCGGCTCCAAATGACCCACCCTCGACCAAGCCAACCTCCTGCTCTGAACCGGCTCGGGGTGAAGGAATGATAAGAACGGACAAAGTGACGAAAACCACACCACCTTTAAAAGCGCTACGGGCAGTGGGCAGCAGGGTGGTTCTTCAGGTACCTCCGCGGGCAACCCCGACTCGTTGAAGCAGGATAAGATTAGTAAGAGTGGGGATAGTTTAAGTGTTGTGGATACGGGTCAAGGCGACGACACCCATTATGCTAATTTGCCACCAAGTATCACCCCCACATCCGACTGACCGAACGCGCTGTCATTCACCAACAAGAACAACGCGCAGCGGGTGCAGCTGTTCCTCCGCGGCCTGTTGGACAGCATCCCGGTGTTGGTGAATAAGAGTGGCCAAGATGATAACAGTAAGTTTAAGGCGGAGCACCAAAAATGAAAGTACTCCGACTTTAAGTCGGACCAAACCAAACTGAACCTCCCCGCTTACGGTGAGGTGAATGGGCTGTTGAATCCGGCCTTAGTTGAAACCTATTTTGGCACGACACGAGCGGGTGGTTCGGGGAACCAAAACAGTACAACCGTCCCCGGTATCGGTTTTAAAATTCCCGAACAAAATAATGATTCAAAGGCTGTGCTGATCACCCCCGGGTTGGCTTGAACGCCCCAGGACGTTGGTAACCTCGTTGTCAGTGGCACCACGGTGAGCTTCCAGCTCGGCGGGTGGCTGGTCACCTTCACCGACTTTGTCAAACCCCGGTCGGGGTACCTCGGCTTGCAGTTAACGGGCTTGGATGTGAGTGAAGCGACGCAACGGGAGTTAATTTGGGCCCCCCGGCCCTGAGCGGCCTTTCGTGGCAGTTGGGTCAACCGGCTGGGCCGGGTCGAGAGTGTGTGGGATTTCAAGGGGGTGTGGGCGGATCAAGCTCACAGCGCGGTGTCCGAGTCGCAAGCAGCTACAAGTGGTACCACCACCGCAACAGGGGCTGCCTTACCGGAGCACCCGAATGCTTTGGCGTTTCAGGTGAGTGTGGTGGAAGCGAGTGCTTACAAGCCAAACACGAGCTCCGGCCAAACCCAATCCACTAACAGTTCCCCCTACCTGCATTTGATTCAACCTAAGAAAGTTACCCAATCCGACAAGTTAGACGACGATCTTAAAAACCTGTTGGACCCCAACGAGGTTCGCGCCAGAATGCTCCAATCATTTGGTACAGAAAATTCCACCCAAGCCCAGCCCCAGTCGCTCAAAACAACGACACCGGTATTTGGGGCCATGAGTGGTACCTCGGCAGTGTGCTTAGTGGTGGGGGTACCACGCAGGGCTCAAGCTCAGGTCAATCTGGCGTGGATCTCTCCCCCGTTGAACGGGTGAGTGGGTCACTAACTATTAATAGGAACTTTTCTTATTAATAAGAACTTTTTTGAAAAAAGTTTTGAATTTATTTTGGTGTCTTTACTAACTTAAAGCAATTTAGTGAACGAATACCCAAAAAGTATGAAATTAGTTAAATTAGCGAGTTTTCACCGCTTTTTAAAACAAGACCAATTGCAACTTGTTCCTTTGTTTAGTCCAAACTTTTTGCAAGTTTTATTGGCGCTTTTCAATAAACCGGTCAATTTATTTAGTGAAAACACAACAATGAAAACAAATAAAAAATACGCCTTTGCGGTGTTACTAATGCAAGCTCAAGCTAAGCTCCCAAATTCATTTAAATTCTTTCTTTCAAAGAAAGGAGGTATGGCTTAATGAAAGAGAAAATTCCGTTTTATAACGAAAAGGAATTTAACGAGATGATGAAAAAAACCAAAAAGGGCACTTTTTCGGGATGATACATCATTAATCCAGAAAACAACAGTGTGGAATTCTCTGGCAGTTTCAATAGACAATTTAAACTTAACAAACCGATTATCCCAGTGAACACGGAATACGTAACGCGTAAAGAGTTCAACGAGTACAAAGACTCAAACGACCAACGACTTATAAAGATTGAAACCACTTTGACCGCCCAAGGTGAACAAATCAACAAATTGACTCAAACTGTTGAAAAACAGGGCGAACAAATTAGAGAACTTCAAGTGGAGCAAAAAGCTCAGGGTGAGCAAATTAAAGCTCAGGGAGAAACCTTAAAGCTAATCTTGCAAACGCTGCAAAAAATGAGTGATCGTTTGGACAAAATTGATCCACCCAAATAGTTTTAGGCTGAAAATAAAAATACTAGCAATTAGTCTTAAAAACAAGCTGTTGGTATTTTTTTATAGATAAAAAGTTAACTGTTTAAAAAAGCAAATGATTAAATTTTATTCAAATACAAATAAGTAAGTAATCACGATGAATTACTTAATCAAATGCTGTAAGTAGGTGAATAAATTAATTTGTTTTTCGTTAGCACTGTAAGTAATATAGTCTTTTAAAAAAATTTTATCCAGACTTTTTCTGCTAAGTTCATCTTGTCCTTTTAATGATCAAATCAGTGAAAGATCAAGCTGAATCCATTCTGTAATTATTTGTTGACTTTTATCTTGATCTTGAAAATTTTCATCTTTAACAATGAAACTTTTTTCTGTAAATTGTTTTTTTGAATGAGATTTAATACTAAGGAAAAGATCGAAATTATGCTTACCGCCATTATTGGCAGTGAAACTTTTTTCTCCAAAATAACTCCCATACCAATTAAAGATATAGCTTAACCTAGCATTAACTGAAAAGTCACTAAAGCTTTTATATTCCATATTGAAATACTCAACTTTCCGCTCCATTTTAAAATCGCTATTTTTTGTGGGGTTAGTTGCTTGATCGAAAGCAAGTTCCGCAATACCTAATTTACCCTCTTCAAAACTTTGTTTTCATGCTTCTAACAACAATTTTGTTGTATCTTTCGGATTAACTTCGTAAGACTTCTTTGAAGAAGTTATTATGTTCTTTTTACCATCTGTAGTTTTAATTGATTTAGACAAATCAATGTCTTTACCGGTGGCTTCTTTAATAAGATTTCTAAGATCAGCTTGGATACCAGTGCAAGCGCTTAAAATTGTTCCACATAACAAAAATAAAGAACCAAAAGCAAACCTTGACAAAAATTTCTTTTTGCGCATCAACAGTAAAAATAGTTCATCAATTCTGCTTTTTTTGGAAAAGCACGCTCAAAAAAGAACTTCATTGAAATTTTCGGTTGATTTTTTTCTTAAAAAAGAACTTTTTTAACTTAGAAAATTTACTTTTGACATTTTTATTCACATAAATAAAAAAAGCCATCTGCCCAAGGTGAAAAAAACAGACGGCTTTTTGACGATTTTGAAACTAACAGCAAGTTGTAACTGTTAGTGATTTGAATGAAAGAAATATATGACCTTTAGTCTTTTTAAAACAAATAAATCAAATGTGCTGAAAGCCATTACGTTAACATAAGTAAATTAAGACATAAAAAATGAACACATTAATCAAATAAGTGTGGGAACAGAAAATCAGGCTTTCAACACAAAATTTTCGTTTTTCTGCTCCCACTAAATAAATTGAGTTGGTTTTTAAAAACCGCCAAAAAGATTAAAGTTTTAACCCCACCGATCCTTATCGGTTTGCCGTTTTACTCGTGCCGCGCAGCGTGGTGTACTATGAGCAGTTGCAGCGGGGGTTGGGCTTACCACAGCAGCGAACCGAGAATGGTCAAAATACTTCAACCACCGGGGCAATGTTTGGCTTGAAGGTGAAGGATGCCCAAAAAGACGACCAGAAGTCCTCGGAATATTTAAGTGGGGAGGCGGGAAGTCAAGCGGGTGGATCCACCCAAGGTACTTCCACCACCACCCAACGTAGGGGTTCGTCAAATGAAAACAAAGTCAAGGTGTTGCAGGTGGCGATGAAAAAGAAATCGGACTCGGAGGACAATGGTCAGATCGAACTTGAAACCAACAACCTCGCCAACGCCCCGATTAAACGGGGCTCCAATAACAACCAGCAAGTCCAACTCAAGGCGGACGATTTTGGTACCACCTCCAGTTCGGAATCTGGTCAATCAGGTACTCAAGGATCCACCCCAAGTAATCCGGGACCCTGAACCCCGTGGTTAACGACTGAGCAAATTCACAACGACCCCGCCAAATTCGCCGCCTCGATCCTGATCCTGTACGATGCGCCTTATGCGCGCAACCGTACCGCCATTGATCGCGTTGATCACTTGGATCCCAAGGTGATGACCGCGAACTATCCGCCCAGTTGAAGAACGCCCAAGTGAAACCACCACGGTTTGTGGGACTGAAAGGCGCGCGATGTTTTGCTCCAAACCACCGGGTTCTTCAACCCGCGCCGCCACCCCGAGTGGTTTGATGGCGGGCAGACGGTCGCGGATAACGAAAAGACCGGGTTTGATGTGGATAACTCCGAAAACACCAAGCAGGGCTTTCAAAAGGAAGCTGACTCCGACAAGTCGGCCCCGATCGCCCTCCCGTTTGAAGCGTACTTCGCCAACATTGGCAACCTCACCTGGTTCGAGCAAGCGCTTTTGGTGTTTGGGATTTGTTTGTCTTAATTAACTAACAAAATTAATAAATAGGTCTATAGGTTATATTAGTACACCCCCTACACGCCCTTTACGACACCGCTCAATGGGGGGCTGGATGTAGTGAAGGCGGCCAATTTACACCCCTCATACGAACTCGTGGACTGAAAGCGGGTGGAGGATACCAAGTTGGTGGCGCTGGTCCGTTCAGCGTTGGTCAGGGTGAAGTTTCAAGATAGTTCGAGCCAACAAGGCACTGAGAGTAATGGCCAAGACCAAAATGCCCTTAGTTTTGATACCACCAAGTCCCAACAAGCCTTACAGCAAGCTGGCGATCAATCAGGAATTTCATCTAATTCCACCCAAGAAGACTTCGCCAGCTACATCCTCATCTTTAAAGCCGCCCCGAAGGCCACGTGGGTGTTTGAACGGAAGATTAAGTTGGCGTTGCCCTACGTTAAGCAGGAAAGTCAGGGTTCCGACGATCAGGTTCCAATGGTAAGGGCTCCCTCTACAAAACCCTCCAAGACCTCCTCGTCGAACAACCCGTGACCCCTTACACGCCCAATGCGGGGTTAGCCCGGATGAATGGGGTTGCTCAGGACAAGGTGCATGGGTTGGAACAAACCACTCAATGAAACCAACAAGCCTCCCAAAAAAACCTCACCAACAACCCCGCCCCCAAGGCCGTCACCGGCTTTAAGCTCGACAAGGGCCGCGCGTACCGGAAGTTAAATGAAGCTTGACCGGTGTATGAACCCCTGGATTCGACGAAGGATGGCAAGGGGAAGGATAAAGATGGGTGAACCACTTCCGGGGCGAGTGAGGCCAAAGGAGATGCCCCGTTGGTGAGCTCAACCGAGAGTCAAATGGCTGCAGTCACAGACAGTCAGCAATCAGGTCATAACAGTGGATTGGTATCGCTTGCCCAGCGCTCCACTACTGTTGCTGTCCAAAAAAGTGACTCTTCTGGTTCTCAAGGCCAAGGCACAACCGACAACAAATTCCAAAAATATTTAAACACCGCCCAGGCCTTGCACCAGATGGGGGTGATAGTGCCATCTTTGGAAACGTGACCTGGTAAACCAAGTACTGGAATCGCTACCCGTGCTGGTGGTGGTGTTTCAGTCCAAGCAGCGACCCGGCAATCGTCTTCTACAAATGAAGATCTGCCGAATGTAATAACACAGTTATACCACACCTCCACGTCCCAACTCGCTTACTTAAATGGCCAGATCGTTGTGATGGGTTCAAACGCGGTACCGAGTTTGTGGTATTGGGTCGTGGATGAGCGAACCACGTCCGGGAGGGCGACTTGATGAGCCCACACCGAGTTGAACTGGGGCACCGACAAGCAGAAGCAGTTTGTGGAGAATCAGCTTGGTTTTAAAGATGACTCAAATTCTTCCTTGACAAACTTCAAGAGTCAAGGCTTAACTCAGCCAGCTTACCTCATCGCCGGCCTTGACGTTGTCCAAGACCACCTCGTCTTTGCGGCCTTCAAAGCGGGCGCGGTGGGGTATGATATGACGACTGATTCGAACGCTTCCACCAAAGACCAAGCGCTCGCCTGGTCGACCACGGCCGGGTTGGACAGTGCTGGGGGGTACAACAACCTGGTGGAAAACACGGCCGGACTGAACGGCCCGATTAACGACTTGTTTACCCTGCTCGACACCTTTGCCTATGTGACCCCGGTGAGTGGGATGAAAGGGGGGAGTCAGAATAACGAAGAAGTGCAGACCAAGTATCCCGTTAAGGATGATAGTAAGGCTTCCGCCAAAATTGCGTCCTTAATTAATGCCAGTCCACTCAACAGTTATGGGGATGATGGGGTGACCGTGTTTGATGCGTTGGGCCTTAACTTTAACTTTAAGTTGGACGAGGCGCGCTTGCCATCGCGCACCGACCAACTGCTTGTGTATGGGATTGTAAACGAAAGTGAACTGAAGTCCGCGCGCGAAAATGCCCAGTCGACCTCCGATGATAATTCAAACACCAAAGTCAAGTGAACCAAACCGCCTCGCACTACCTCCCCGTGCCGTATTACTACAGTGCCAATTTCCCCGAAGCGGGTAACAGAAGAAGAGCGGAGCAGCGGGAATCATTAGCATTTAAAAAGTTCTTTTAAAACAAATTTGAAATAAAAAGGATTCAAGAAATAAATTTCTTGTTTATTGTGAAAAATTCAAAAAAGCAACAATTTTTAAAGTGATTTCGCTTTGATTACAGACCATTTTTCAAAGCAAAATTAAAAAAGCTTTTTATCGTTTTTTGCATATAGAAAATTCAGCATTTATTAAGCTCAAAAAATAAGAAAAGCAGCAATTTCATAATTGAATTAAGTTTAATTCGCTAGTAGTGTTAAAAAGAAAAAAACTGCTCCAGGGTTTCTTGAAATTTTTACCGCTTATCATTCCCGCAACCATTTTTGTTAGCTGTGCAAGACGTGAAAGTAATCACCTCATCTTCAACATTTCACTCGATCACGACGCTGATGCTTCTATTAGTAAGTTCTTTGAACTTTACTCTAATAATTTAAGTAAAAAACTAGATAAAAAGGTTACCGTTAGCTTCAACATTATTGATGACTCTTTTACCAAAATTAGCAACATTCAAACTGCTAAAGCTGACTTTGCTTTCGTTAATTCTCAAAGTATTAAAGATAACGGAATAGAAGAATTTAATCTTATTCTTCAAACTCAAACTGATGCATTTAAGGAAGACACCAATTTAGATTATTACAGTGACGGTCAGTTGAAATCTAAAGCAGAAAAAATGACCACTTTATTTTCAAAAACGCCATATAAAGATTGAGAAGATACCGCACAACAATGAACCGGTAGCCGCTATAACTTTTTGTATGAAACAAACAAACTAATCAATTTCTACAGAGGCATGATTTTAATCACAGGTAGTGAGGAAGAAATTAAAAAAATTAAAGAAGCTTGAGATCAAAAGAAATGAAGTGATTTCATGAATTATGGCATCGGTCATGGCTCAAGTGGTTCAGCTGGAAAGTTCCAACTACCTGATCTTTTATTAAGAAAGCACTTTGGCTCTAGTTACCCCGGTTTGCAAAATGCAATTAACCAAAACCCTGATAAGTTTGCTAATGTTAGAGGTCGGGAAATAGGTAGAGACAATAAAATCAAAATTGTCTTTGATGATGCTAATTCCTTTGCTTGAACTCACAACGACAAGAATGCAACTAATCACTTTTATACACCAACAGAAAATAATGGCAAAGGTGATTCAGAAAAAAGCAATAATAAAAATAATAAAGTCGAAATCTTAACCTATACTGATCCAATGCTGTATGACATTGGCATTGTAAGTGATACTTTAAGTGATCGCTATCAAAAAGCAATTGCTGAAGTTTTTGTGGAACTAGCTAAAACAAAACAAGATATTTATGGCCCTTCATATGGTTACAACGGTTACAATTTGATTACGGACCCTAACAAAGAAATCTTGGATGTAATTCATAAAACATATGGTTAATCCAGTCTTAGTTTTTGATCAAGTTTCCCTTAGATATAACGGAGCACCACTTTTAGAAAACATTAACTTTACAATTTCACCTGGTGAACACATTTGCTTGTTGGGTAAATCTGGTGTTGGTAAAACTAGTCTTTTAAACTGCATTACTAACACCAAAACAATTTCGAAGGGAACCATTTATTTCAATGGTATTGCTTCTAATAACAAGGATTACAAACAATTAAAAAAACAGTTCAGTTTTTTAGATCAAGTACCTAATTTAATCGACACTGACTTCGTTTATGACGCCATTTGAAGGGAAGCGAAAAATAACCTGAAATGGTGACAACGGTTATTTTTAGTGGAACCTCAATCGCTAAGAGAACAAATTATCCAAATCCTTGAAGAGGTTAACCTAAAGGAATATGTCACTTACATTATTAAGGACTTGTCAGGTGGTCAAAAACAACGTGTGGAAGTAGCTAAATTGTTCTTTGCCAATTCCCAAGTTTTATTAGTTGATGAGCCCACTACTGGACTTGATTTAATCAATGCGCACAAAATCATGGAACTAATTATCCAATTTGCGCGCCAAAAAGCAATGACCTTAATCTTTGTGACCCATGATGTTGAGTTTGCGCTCAAATACAGTGACCGTATTATTGCTCTAAAAAATAAGGCATTAGTTTTAGATCAAGCTACAAATAAGCTTACCAAACAAAAGTTAATGCAAATTTATCATGACTAGCTTGTTTTTCTATCAAATATCAGATAAGCAAAAGCGTTGAAATTGGTATTGAAAGTTAGCTTTAGCGATTATTGTTTTAGTTGTTATTATCTACAGCTTTATCGATAACTTTTCCGGTTTTAACGTATCTGGTTTTAGGAATTTTAGTCGTAATTTTATCCGGTTATTCACCCCTGACACAGCACGTGATTACTTTTTGGGCTCTTACCTACTGCAAACCATTTTTTATGTCGTGAGTGGTAGTATTTTGGGATTTGTCATAGCCCTGTGGTTTTCGTACCTCACTGCCTTTAAAATCCAGCCACTTTACATCGCTTTACCAACTCGCTTATTCACCATTTTTCTGCGTTCCTTTCCAGTACTTGTGTTTGCTTTTTTGTTTAACAATTTGTTTAACAAACAACTAACAGCAACCTTAACGATTACCTGGTTTAGTTGATTGTGAAGCACTAAGTACATAACTGCTTTTTTTGAAAACAGTGCTTTAAAACAATTTTTTAACCAAAGCTCTAGATATATACATAAATTCAAAGCTTTTTGGAATACCGTTGTTATTTCCCAAGCTGAGCGCTTATGGTTGTTTTTGCTCTACAGCTTGGAAGCTAACTTTCGCTGAACAACAGTTTTGAGTATTGCTGGCATTACTGGAATTGGTGAGCTCATTGCCACACCTCTAGGGGGCACAGTGCAGCTTAACTTAGTTTTAATACCGATGTTAACTTTGATCGGCTTTTTACTGTTTTTAGAAGCGAGTGTTTTTCTGTTAACTAAGTTTGTTTTACAAAAACAGAGCCAAGCCGGTGATTACTTTTTACAAGCTAAAACACTGCAAAAGAGAAAGTGAAAAAAAGTGATGATTTACATCCTAGCTTTGGTTTTAGCGGCTTTTACCTTGGCTAATTTAGTACAGTTGGACTATACGGTTAAAGCGCCAGGGTTTGTGGCTGACTTTTTTAAACAGTTCTTTCAAACAAAAACAGCTTTTTTAATTAGTGAAGACGCTAACATTAACCCACTTTTAATGTTGTTAAAACTCACAACTCAAGCGATTAGTTTAATTACCTTAGTGTTTGTGTTAGCACTCTTGTTTGGCTTTTTAGCATCAAAGCTGTTTTCCACTATAACCTCAATTAGCTTAAAACTGTTACTTTTAGTGATTAGGGTAATTCCCAGTGTCTTACTGTTCCGCTTATTTGATCCAATTATTTTTCGCCCAGAAACTACCATTATCTTTGTTTTAGCAATCCACAGTGCTGCTAGTTATGGTCAACTGATAACGATTAACTTTGATAATGCCAACGAAGGGGTAATTAACAACATGCAGAACCACGGCTTTAGTAGGTTTTACATCTTGTGAAATTACCTCATACCAACCACTAAGCCCCAACTGTTAAATACGTTAAGTGATAGCTTTGATAATGCTATCCGCGACTTAGTTGTGTTTGGGATCTTTGGTGGTTCGATTATTGGGGGGCGAATTAACAATTTCTTTGAGCGAGCACAGTACTCTGAATTGGGCACAATAACCTTACCCTTAATGGTTTACTTAATGGTGTTTGAAGTCATTTTAATGGCAGTACGGTTAAACAAACTCAAAGTGTGACAGCGCCATCTGTGGTAGTTAATTGGGTTTTTGTTGGTTTAAGAAACGCTCTAAGATTAAGGTAGCCGCTAAGGTGTCCTTGGCCTTTTTAAAGGTTCTGTTTTTTAAGCCTAATTCCTGCAAGTTAGCTTTTACCTCACTCGTAGTACCACTTTCATCAATGAGTGTTACTGGTACATTAAAGCGGGTTTTTATTAGTTCCACAAACTTGTGAATGCTCACTTGGATACTTGACTGGTAGTGGAAGTGGGGAAAGCCAATCACTATTTGCACTAATTCATAACCAGCTTGCTTTAAATCCTTAAAAAGGGTATTAACGGCTTGTTGCAAATTATTTTGTACCGCAAACACGCGGAAGGGACTTGGAAAGCGGTCAATGGTATTGACTAGTGCTGTACCAATTCTCTTCAAACCAAAATCAATGCCTAAAATGTACTGCACTATTGAAGGGTATCGAGGATAGCTTGTAGTTTACTTACATCCACCGACTCGTTAAACGAACCCCTAAAGAAGTTATTACTACCCCCACCCTTAAGTTCAAAGTGAGCTTTTAAAAGCTGAATACATTTAGAAACATCTTGCCCTAATAACATAAAGGACGGACTGTTGTTGAATTGGTTGAGGAGTACAAACAGCTTGTTTTTGTGTTGGTTCAAGACTGCTTGGGCTACTTGTGATAACAGTTTCGGATCAACTGCTGCAAAGCTAGCGAGAACATAAGCTTTGTCTTGCTTTGCTAGCTCTAACAATTCCTGTTTGATCTTTTCCGCTAACTGCTTTTGCTTCCACTTAAAGTCTTCCGTTTTCAGTTCATTAACCGTTATTTTGAGTTCATTTAGTAAAGCTTGGCATTTACGTAAATCAGTAATTACTTGCGGCAAGTGAAAGTGATTTAGTTGCTTAGTTAAAGCAGTTTTTTGCTTGTTCAAAGCGGGGTTAGCAATGTTGTTTAGTGTATTGTGTAAACTGTTAATGGTTTCACTTAACTTGCCATTCTGCTCATTGAGGTAAGCTGCAATTGTTTCGTTACTACTAATGATTTCAATCCGTCAACGCCCTGTGCCACGAGCATAGTAATCAGTAATAAAACAGTTCTCAATTAAACCAGTGTGTTCCACATGGGTACCACCACAAAGCTCGACACTAAAATCACCAAAGCGAATTACCCGTAACAACTCGTGGTTCGAATACTCCTCTTCAAAGTAGGCAATCGCATTAAGTTTTTGACTACCCTCTAGATCAGTAAAGATCTCTTCGCGCTGAATGTCTTGTTGAATAATTTGTCGAATCCTGTGTTCCACCCGTTCCAGTTCGGCTCAGGTTAAGGCTCGCGAAAAATTAAAGTCAATGGTAGCTTTTTGCGCTGACTTAAAGGCGCCGTCTTGTTTAATCAAGGGGTCAATTTCATTTTGCAATGCCGCGTGCAGTAAGTGTTCTAAACTGTGGTTGTTTTTCACTAGTTGGCGCCACTTACCGTCATGTACTAGGATCACTTTGTCACCTAGTTGAAAACTACCAGTTAAAAAGAAGTGGAAGTGTTGTTTATTAGGGCCTTTAAACACGCCCTGAAAGCTTACTCTTTGATCATTTTGGCTGTGGTTAATACAGTACCCCTCATCATAACGCTGACCGCCTGATGTGGCATACAGCACCGTTTGGTCAAACACCACATAACCACTTTGGTTGTGCAGTTTTTGCACCGGTTGGTACTGTTCATCAAACAGCCCAATGACCTTGGCGGTGATCTCATTAGCCTCGTATAAGAACTCACTCGCTGTTTTAAAGTTCACTAAGTGTTCATTTTGCTTTTCAAAGCTCAACGTATTACTGTTTTGTTTGGAGATCAAACGGTGCTGTTCCATCAGTTGTTCCACGGCCTTTCAGTCTACCTGGAGTTGGCGCTTATCCACCAGTTCCTTAATCATTTCAATCGGAAAGCCATAGGTTTCGTACAACTGGAAGGCTACTTGGGGTTGGAGTGCATTATGAGCAATACTCTTTTCAAACAGCATTAAGCCCAAGTTAATTGTTTTATCAAAGGCCGTAACTTCCTTTCAGATTGCTTGTTGCACCACCTGGTTTTTCCCCTGCAACTGCTGGTAGTAAGACCCATAAACGCTAATAATGGCATCGACCATCGGATCAATAAACTTTAAGTCTAAGTCCAGTTTTTTGCAGGCCAACAAAGCGCGGCGCAGTAACCGGCGCACAATGTAACTGCGCTCGTTGGGTCCTGGCAATACGCCTTCGGCAATGGTGAAGGTAATTGCCTTAAAGTGGTCACTAATAATGCGAAAGGCACTTTGCGCTTCGTTTAACTTTTGGTCAAACTTAAAGTATAAGTTGGTATCATACTTATGCTTACAGTGCTGTTCAATAATGCCAATTAACTTTAAGAAAATATCCGTGTCAAAGTTAGTCGGCGCGTCCTGTAAGATTGCTACTAGGCGTTCAATACCGGCCCCAGTATCGATGTTCTTCTGCGCTAACTCACTGTAGTTACCATTGCCATCATTGTTAAACTGACTAAAGACAATGTTCCACACTTCCACATAACGGTCGTTTTCAATGTCCTCAAAGAACAGTTTTTCACCAACCTTGTGCGGATCAAACCGCTCCCCGCGGTCATAGTAAATTTCCGTACACGGTCCACATGGTCCTAAACCTAAGTCCCAGAAGTTGCGGTCGCGGTCACACTTAATAATCCGACTTGCAGGAATACCGTGTTTAAGTCAGGTGTTAAAGGTCAATTCATCATCTTGGTACACTGTAATGTAAAAGCGCTTCGGATCCAAGTGGTAATGATTGACTAAAAGATCATTGGCAAAGCCAATGGCCTCTTCCTTAAAGTAATCACCAATCGAGAAGTTACCCAACATCTCAAACAGCGTTTGGTGTCGCGATGTAAAACCGACGTTTTCAATGTCATTAACCCGTAGACAAATCTGGGCGTTGGCTAACCGTTTACTCGGTGGTGTTTTCTTAGCACTAAAGTAGTCCTTTAAGGTCGCTACCCCGGAGTTAATCCACAGCAGTGAAGGATCATTTACGGGAATTAACGATTTGGAAGGGAGTGCTAAGTGGCCCTTGGCAGTAAAGTAATCTAATCAGGTTTGTCTAACCTTATCAGTTGTTCATTTCATTGGTTTAATTGTAAATGTTTGCCTTAAACCAAGGATGACCACACAACAAGCTTACACTTCAAACTTCTTGTTGGCAATTTGTGCATACACCATTCGATCTTGACGGAACTGGTGGAATTTAACCTCACTGTTAAGCGTTCACAGCCCTAAAGGTAACTGCAAGCTCAACACCATGTCCGGGAACTTTTCGTAAATGTTTGTTCAGGGATGGAGATACTGACACACTTGCTTAATTTTGAGTGCATCAACTGCTTGGAACTGCTTTTTCGTTCAAAACAAAAAGGCTATTTGGTAGTTGGGGTTAATGTCATACAAGCGCTGTAAGGAAGCAAAGTTAAACGAGGAGAACACTACCTTTTCAATTACTTTTTTGCCATACTGTTGGGCCAAAGCATCAATTTTAGCTTCAATTCCCGGGTATTCCTTTTGGTCAGTTTTAATTTCCACGTTAATTAGTTGAAATTTATCTAAGTACTGATCAAAGAACTCCTTTAAGGTCATAATCGGCTGGGGTTGCGTTTTGAACTTAAAGAAAGCACTGTGATCATCTTGCTTTAAACTAGCGAGTGTTTCCAGTTCGATTGTTTTGTCGACTAGCGCTGTTCTGGTGGTAGTTTCATCATGGATAATCACTAACTCCCCGTCCTTGGTCAAGTGCACATCTAACTCCACGCCATCAAAGCGATATTGGAACGCTAATTCAAACGCTAGCTGGGTGTTTTCAGGCGCAATATCACTGTACCCACGGTGTGCTAGCAGAAGTTGTCGTTTAAGCATTGTCTAAGGTTCTCCAGCGGTAATCGGTTTTACCCAAGCGCTTTAACTCTCAAGTATTAACCAAAAAGACAATCGTACCACACAATAAACCAAACAGGGCTATTCCTAGACAAATAGCTGCAATAATTTGGTAACCCTGAATCGAGGTATTCTTCTGACCGTCTGGGGTATAAGCCTTACCCACGACACTTGTAACCGTGTAGAGCCAACCATCAGTCGAAAAACCAATAAAGGAAAGTAAACCAACACTAGAAGCATAGTTGTTTTTACCAATCTCTATTTCCGCTACCTGGTTGTACCTAACGGTTACCATACCCCAAGACAACACCCCGGTGAGAATGTAGAGGATCGCCGATACGATAATCAAGGCATAGTTTTGCTGTTGGCCCACTTGCCCAAAACCTAAGAGAATAAACATCAAGACAAAGATAATGCCAAGTCCTGTACACAACATTAAAAAGAGGATGTAGCTCCTAAACTTATCAGCTAACCTGCCCAAGTACACACTTACAGCACTGCGCAAAACATACGTCCTTACACCACCCAGTACAGTCCCTAAGATCACTGGTGCCAAAAAGGCGTTTTGCATCATTTGTAAAAGATAATAAGCAAACGTACTTTGAAAGACATACATTCCCATTAAAAAGAAGGACAAGAGTCACAACTTCCAGTTCTTTAAGGTAATGAGAATCTGTTCAAAGTTCTTTTTGGCAGTTTTTAAACTGGTATGACTGTCGTACTTTTCAATTGGTTTTTCTGGTACAAAGAACAGCACGGTAAACCCAGTAACGAGCAACATGCCACCAATAATAAAGGCATAAATGGCAAACGGTGCGGGATTATTAGCACTACTTTCACCACCGGCTGGGTAAGCTACTGCTGTAATAATCAAGGCAATTAAGAAAATAAAAATAAAACCCCAAACACCATTAGCAGCGCCCTGAATACCAAAACCCAATGCTTGGTTTTCCTGGGTTGCTTGTTGACTCGCTAACTTTCACAGTGGTGTTCAAAAGATTAAGGTCGAAGTAATACCCCAAAGCCCCCAAATAACACAGTACTGTACAAACAAGGCATTGGATTTTTGGCTTTCACGAGTAAGAATGTTAGTAGCTAGCCAAAAGGTAATTACACCCGTTGAAATTTCTGACAAGAACAATAGTTTCCGCGAACTAAACCGGTTCGCTAAAAAACCACCAGGTAACTGCGTGGCTAAGGTCACATAACCAATAATCGAGGTTAAGGTGGCCACTTCATCTTCAGTAATCCCTAAATACAGGTGCAAGTTAGGCACCACATTCTTAATATAGTACGGGGCCGCAATTACAAAAACATCAGCCGCACCGAGAATAATTAGTGCTATGAGCTGCTTTTTGGAAAACTGCTGCAACCGTTTCTTTAAACTAAGTTTCGTTTCCATTGTGACACAACGAAATTACGCCATTATTTTAGTGGTAATTATGGGGTTAATCCGGTTAGCTTGCCAAAAAATAAATTAGAAAAACGTTTTTGGCATACATTTATTGCAAAAAGTGAGAGGAAAACCGTATTTAGATTTGGCTAGCGGTAATGATACCACTCCCCAAACAAATGCCATTCTGGGTATACAAAACACCATACTGACCGTTGGTAACGCGGTCAATTGGTTGATCTAGTTGTACTTGCACGACGTTGTTTGGTTGTACTGTTATGTGCCCTTGCACTAACGGTTGGGCATGGCGAATCCGTACCAGAACGGTTTGGGTCAATGGATTGGGTTGGTACAACCAGTTAAAGTGGGAAAGGGTAATGCCACGACGCTGTAAATCTTCTGGGTCACTCGAGACATACAAGGTGTTTGTTTTTAAGTCCTTTTCAACGACAAAGTTCCGTGCTGCTTGCCCACCTAAATTTAAGCCACTGCGCTGCCCAGTGGTGTAAAATCACACCCCTTCATGACTGCCTAGTTGGCGCTGCGTTTTTCAATCATAAATAACCCCCGGTTTGACTGGTAAATAATTCTTTAAGAAGTCACTAAAGTGGCGTTCCCCAATAAAGCAAATGCCGGTTGAGTCCTTTTTAGTAGCCACACTAAAGTGGTGTGCTAACGCTATTTGCCGCACCTCCGTTTTCAGTAAATGCGCCAAGGGAAACACCACGTCTTGTAGTTGCGCTGGTTTGACATGCGCTAAAAAATAGGTTTGGTCCTTGTGCTTGTCTTTGGGAATGGCCAGTAAAGGTTGGTTGTTGTCCCAGCTAAGTGCCGCATAATGCCCTGTAGCAAAGTCACTGTTGGGATCTAACTGCTTGCAGTAATCACGCATTAGCCCAAATTTAATTAAACGGTTACACAACATGTCGGGGTTAGGGGTTAACCCGTTTTTAAAGGCATCAATGGTAGGGAGAAATACCTGCTTTCAGTACGGTTCCACCAGGTTTTGCTTAATTAGTTTGATTCCTAGTAATTGGGCAATTGCTTGCGCTTCGCGAAAATCTTCTTTGGACGAACAACCCGACTTATGCTCGTTAAAGGCGCGGTGACCTAACTGGTCGTTATTTAGTGTATTGTCCCAGCACTCCATAAAAATGCCAATCACTTCGCGGTACTGTTGCTTTAAAAGGAGCGCACTCACAGCTGAGTCCACTCCACCTGATATTCCGACAAAAACGGTTTTATTTTGCATCTAGTAAACTGTTAAAACACCAAAGCGCTTACCCAACTGCTGGTGGTAACGCCCGACATCAACACCCTTAGTTATGGCCAGCGCTGAAATAAATAAGGTTAACGCTTCATAAAAGCTCTGACAACAACTTTTGACATCACCTTTAGGATTGTCCACTAAAACAGCTAACAAATCCAGTTGTTTGACCACTTCACTACTAATAAAAAAGGCGTCACTGAGCATCGGTTTTAAAAAGAAGGACTGATCATCATAATCATCATCTTCATAAATTAACAGGGTACTCTGTTCGCGTTCCAAACAACGATTAAAGTTTTGGCGAAAGGTCTCAAAATCAACTTTTTCAAACTCAGTTTGCACAAACTGCTTCAAGGCTAAAAGTTGTGGTTTCATTACCTTAGTTTAATAAGTTGCTCGCGTAATTGGGGATCTGTTATTTTTTCGTACAACTTTAAACGGAGCTGTTGCTTTTTAAACAACTGCAATTTCGCTTCAAAACGGTTAAAGATCAGGTTGCAGGTTTTGATGTGTTGGTGGATTGAACTTTTCTTAACTTGGTATTTATCAGCAATCTCTTGCATGCTCAAATCCAGGTTAATGTACTCGTTAAAGTAACACGCTTGCCGTTTGGTTAAAAGTTCCCCGTAAATATCAAATAGTTGCTGTTGCTTTAAACGCTGGTTAAGTTTGGTTTGGTTCATGGTTGAGGCTTAAATTCTTGGTAAGACCCAGTACATATTGCTCTAAATCAAAGGCCGCTAAATCAGCAGTTGTTTCTCCAAAACCAATCAGTTTTACCGGTAAGTTAAACAGGTCTTTAATCGCTAGGATAATCCCACCCTTAGAGGAGCTGTCCATTTTGGTAAGGATAATCCCGGTAAGCTCGGTAAATTCATTAAAGACCTTGGCCTGTGCTAACCCCGATTGTCCGGTAGTACCATCTAAAACGAGCAGTGTTTCTTGGGGTTTAGCGCTACTCACCTTCTGGACAATTTGGTAAATCTTTTTCAACTCATTCATTAAGTTGGTTTTGTTTTGCAACCGTCCCGAGGTGTCACACAACACAAAGTCGTATTCGTTTTGAATGCCTTGTTGCACCCCACGGAAGATCACCGCTGGCGTTTCCTCTTTGGGATTGGGTAAAACAATGTCACAACCTATGCGCTGCGCTCATTCTGCTAACTGGGCCACTGCCCCAGCGCGAAAGGTATCCCCAGCGACCATTAACACCCGTTTGTTTTGCTTGGTAAGCTGGTCAGCTAGTTTGGCTAATGAAGTTGTTTTGCCAACACCATTAACCCCGACAAACAAATACACGTTGGTTTTATTAGCTTCAACCGTTAAATCAGTTTCAAACAACTTATCTTGGATATAGTAAACAATTAACTTATCGACAATAATTTCCTGGATTAACTGAGGATCACTCACTCGTTGTAGTTTGAGTTCTTGGACAATCGCATCACAGATTTTAGTAGCCGCATGGTAACCAATATCAAGCAACACTAACTCCTCAAACAAACTTTCTTGAAACTGTTCGTTAACCGTTACATAGTTAGCTGCTAGTTTATTAATGGTATTGGCAAAGCTGTTAGCCGACTTGTTTAAACCCTGGTAATAGCTCTTTTGGTTGGCTTGAAAGAAGCTCTTTTCCTGGACAGCTTGTTGCACTTGGTCGACCAGTTTCTTTTTCGGTTTAAACTTCTGGATTAGCTTGTGGATAAAACTCATAACAATTAGTTCTGCTTATCTTTACTAATGTACTGCTCTGCTTTTTCCAAAGAAACGGCAAACGTCTTGGTCACTCCCTTGGTCTGCATTGCCGCTCCCAGTAACATGTCACACTTCATCATCGTGCCCTGACGGTGGGTAATAATTAAGAACTGGGTGTTGTTACTGGAATTGCCAATAATGTTAGCAAAGCGCTCCACGTTCGCCGGATCTAAGGCACTCTCCGCTTCATCTAAAATTACTAGAGGAAAGGCACTAACTCTCAAAATACTAAAGAGGACCGACAAGGCCACGAGTGTTTTCTCCCCACCCGATAACAACATTAAGTTAGCGACATTTTTGCCAGGCGGATTAGCAAACACTTCTATGCCTGACAACAAGACGTTATCAGTATCCGTGTAGCGAATTTGACACGAACCACCGCCAAAGAGGTAGTTAAAGGTTTTAGGTAGTTCTGCATTAACTTTTTGGATGAGCTCGTCAAATTCCTTGCAGGCCAATTCATCAATTTCACCAATCGCGGTTTGCAAATCCTTAATCGCCGTTTGCAGTGATTCATACTCACCGTTAATGTCATCAAAGCGCTTTTGCTTATCAGCAATTTCAGCAATCGACTCCAGGTTAATTGGACCCATTTCATCGAGTTGGCTCTGCATCCTGGCAATCTTGTTTTGCGCTTGCATGGTGGAGAGCTTAATTGGTTTGTTATAGTTAGCGATCGCAAACTCCATCGTTAGTTTATACCCACCGTTAATCTTGTCGGTAATGTTGTGGATTGTGTTTTCAAAGCGGATTTTGCCTTCGCGTGCCAACACTAACTGGGAGCGTTGTTCATCTAAAAGGGCACGGAGGTCAGCGGTTTTGGCTTCGGCTAGTTTAATCGATTTGGCCAGTGTTTCTTTCAATTCCTGGTTGAGCTTTAACTGTGAATTGAGTTCGTCACGTTCAGCCCAAGCACGGTTTAATGATTCAACCAATCTGGTTTCATCAAAGCTGTGCGGGGTTCCGTCAAAGCTTTCGTTGGTGAGCTGCTCATACTCAATCTTATAGCTCAAAATTGCCTTATTGGTGTGGTTAACACGTTCGTTAAACTTTTCCAGTAGGATTTCTTCGTACTTTAACTTTTTGTTGAGCTCATTGAGTTTAGTCTCTAAGCTAGTGAGATTGGTACGTAGTTGCTTTTCGTTATGTTCTAGTTGGGTAATGTTAGCTTCGCTAGAAGCTAACTTTTCCTGGAGGTTATACAAGCTTAAGTTGTCCTTGACATAACCACCCTGGATAATCCCTCCAGCGTACACCGTTTCCCCGTTGAGGGTGACAACGCGGTACAGCTTATAGGTATAACTGGACAGTTTTAAAGCGGCTTGAAGGTCACTGGCAATAATTACTTGACCCAATAAAGCATTAACAATTGGTTGTAAGCTTTCATCACAACTCACATGGTCACTACCAACACCCAAAAAGCCATCGAGTTGTTGCAAAATTTCCATGTGCTCGGGAGCTATCTTAGTATCAAAGGCAATGTCGTCAATTGGTAAAAAGGTAACTTGACCAATCTGGTTAGTTAACAAAAAATCAATTGCCTTTAAAGCCGCCAAGTTGTTGTTAACAACCAGATAACCAATTGATTTACCCAACGCCTTTAACAAGGCCTTTTCGTACTGCTTTTCAAACTTTAAAAAAGTGTTGAGCGTGTTTAAGAGACCGGTGAGGGCCTTGACATTTTTGACCAAAACATGGGCCGGATTCGTTTTGACCCACTCCTCGTTTTGCGCTTTTAAAAACTCTTGCTGCGCTTGTTGGTAGACTAACGCCGCACTGTTTTGGTTAAGATTCAACTTTGTGGTGTCCAACTCAGTTTGGACGGTGCTCAACTCATTGGTTAACAGGGTAATTTCACTGGTTGTTTTGAGTACTTGCGCTTGTAAGCTTTCTAGTTGCGCTTGGTCTACCCGGATTAACTTTTTTAACGCCTGGATTTTGTGCTTTTCGTCCTTTTTGGACAACTCGTTTTTGAGCTGCACATCAATCACGGCCTTGCGTTGTTCCAACTCGTTAATCGTTTGGTAGACCGCTTGCAGTTCTTGTTGTAGTTTGATGGACTGTTCATCAGCATCACGAAAGCGCTGGTTAAAGATGTTGAGCTGGTCTTCTAGCAGTTGTAACTGCGGTTCGTGTAGTTTAAAGTCCTGTTCGATGTAACCAATTTGGTGGTTAAAGCGGTCTAACTCACCCTGGGACTTGAGGTAATCTGCGACCAAGACACTCAGCTCCAGCTCCTTTAATTCTTCCTTAAGTTTGACAAAGCGCTGGGCCTTGTCCGCTTGAATGGTAAGCTTCTTTAAGTCCTTTTTGAGTTCATTTAAAACAATGCTGACCTGTTTGAGATTTTGCACGGTACGCGCTAATTGGTTGGTAACCTCTTCTTTGCGCTTAGTGTACCGCCCAATCCCCGAAGCGTCCTCAAAGATCTTACGTCGCTCCTCTGGTTTGGCTTCCACAAATCACGACACACTGCCCTGGGAAATAATCCCCAACGAGCCTTTTTCCAAACCAATATCAGCAAAGATCCCACTAATTTCTTTTAGCGTTACTAACTCGCCGTTAATATAGTACTCACTCTGACCACTACCACGATAGATCCGGCGCATCACCGAGATCTCTGGTCGGGGATCGTGCAGTAGTTTTTGGGAATTGTCAAAGGTCAGTTCCACTTCCGCTAGCTTACTAGCGGGCTTGTCCTTAGAACCAAAGAAGATCATATCATCACCGGACTTACTGCGCAAGTGCTTCATGCTGCGTTCCCCTAAGACCCACTTCAAAGCATCGACAACGTTGGACTTGCCAGAACCATTAGGACCGACTATACCGGTCATTGAGTGGGTAAAATTAATTGTTATTTCGTCAGCATATGACTTAAAACCATACGCACGAAACCGTTTGAGAAAAACCATTTAAATCAGGTTAGATTTTGCAATAGTGCATTACTAGATTATACTAGTTGGCTTTAAGGAAAGGTTTAGCGCCCCTCAACTAGTTGCACCAACCACTTGTCTTTGGCAAACTAGTACTTGTAATACTTACTTACAATCTAACTATCATGAGCGGTTCAGTTAAAAAAATTAAGCTTAATTAAGTATGACAAAGACAAGTAAAGCATCCGGGTTAAGCTGGTTTTTTTGTGATTTAGATGGCACTTTACTGCGTTACCAAAACAACCAGCACTTAATTGAACCAACTACGAAGCGAGCAGTGGCACAGTTAGTGGAGAGTGGTGCCAACTTTGTTGTAGCTACTGGACGCAAACCGAGTGACGTGCGCAACATTTACAAGGAGTTGGGGATTGAACAAGCCAGTCCCTACTTAATTGCCAACAACGGCGCGGTAGTGTGGGATTTAAAGCGCAATAGCTACCTCAACAAGCAAACCTTGTCTTTAAGTGACTTTGACTTAATTGACCACATTAACCAAACGTTAAACCAATTAAACCACGAGTATGGCTGCATTCTGTATGGCCTAAACGACCAGGTGTACTTTTATCATATCCACGCTCCTGACTCACAGGCCTTCAAACAGTACTTTGCTTTTTATGAAGGCGAATTTGTCCAAAACCAGTATCTGGAAATAGATGGGCTCAAAACTGAATACAATCTAGTTAAAGCCATTTGGTTCTTTAAGGAAGTGCACCAGCAAAAGGCGGTGATTGCGCAACACTTTACTAACCAAGAACGCTTGGTCATTACTTCAGCACACTCGTTTGAACTAGTACCGCTTAATGTATCAAAGGGTCATGCGATAAACCTAATTAAACAACAAGTAAAAATCACCGATAACCAGATTATGGTCCTTGGTGACTCTTACAATGACTTGCCAATGTTTCAACATGGTGTAGTTAAGGTAACTAATCACCTCGCACCCGACAACCTCAAACAACTAGCTACAAGGGTTTATGAACTACCAGCTTCACTGTTTGTGGGTCAAGCGTTGAACGACTACTTTAAGTTCGATTAACTTATTTATTTAAGTGTTTGCGCTGCGGTAATAATGCCCACGTTAAAGACATCACTGACACTGGCACCACGGGAAAGGTCGTTAAAAGGACGTGCTAGACCTAAAACAATTGGACCAATTGCTTCATAACCACCTAAACGTTGCGCAATCTTGTATCCAATGTTACCAGCATCTAGGTTCGGGAACACATAGATGTTAGCTGATCCATTCCAAGTCAAATTAGGGGCTTTTTGTTTGCGTACCTTTTCCACAAAAGCACTGTCAAACTGTAATTCACCACAAACATTAGCTTTGAGTTCGGGGTGTTTTTCCAAGAAGATTTGGGTCGCACTGACTACTTTGTCCACCGCTTCCCCTTTACCACTGCCCAGTGTTGAATAGCTCAAAAAGACCATCTTGAGTTCTGGTTCCCCCATACTTTGGGCAAACTTAAAGGTGTTTTCCGCTACAACTGCTAATTCTTGGGGTGAAGGGTAAACAGCAAAAGCACAGTCGGTAAAGTACAAGCGTTCCTCATTCTTTTCCATGATGAACACACTCGACACAAAGTTACCAGTACCTAACAGTTGTAAAGCAGGACGGAGAGTATCCTTCGTAGCGTATTCCTTACCACACACTTCCCCATCCACTTCGTTGAGTGCTACTAAAGTGGCTGCTAGGGAACTGGCATCACGGACAAACTTTTGTGCTTCACGCATTTCCATGCCCTTGTGCTTGCGCTTTTCATAAACGAAGTTAGCGTACTTTGTCAAGTCCATTTCCTCAATCACATAGTGCTTGATGGCGGTGTTAAAACCAGCTGGTACTTCACTGCGGGTGCGGAAGATCACCGCTGGGGTTAAAATACCCGCTTGCTGGAGCTGGTTAACTGCTTCCATTACGGTTGGCGATCAACCTTCAGGGAAAATGATCACTGGCTTTTTACCAGCACTTTGAACACGTTGTTTTAAAAGATCAATAACACTCATAAAAACCTGCTTACTTAGTTTTACTCTTTACTTTAATTTACTTTTTTTGAATATCGCTAATTCCCACTAGCTCTTCCCCACCTATTAGTGCTAAAGATGCACCACCACCAGTGGAAATAAAACTAAATTGATCAGCAATCTGGAGTTGTTTAGCAGCGGCTGCAGAATCACCACCGCCAATGACACTAAAGGCTTGTTGGTTTTGCGCAATCGCTTTACCAATTGCTAAGGTACCCTGAGCATAGTTGCTAAATTCAAATACGCCCATGGGACCATTCCAAAAGATTGTTTTGGCCTTGGCGAGGTAACTGCTAAACAGGGCCACTGTTTTGCTACCAACATCCAAGGACATAAAGCCCTGAAGTTCTGCTGGTCATTGGGGGGCTACAACACACTCCACGCCTGGTTCATCCTTAAAGCTAGCATGAAGCATTTGGTCTACCCCCAAGACAATTTTCTTGTCCTTGTCACTGTCAAGGATTTGCTTGGCGGTTTGGACTAAGTCTTCCTCCACTAAGGAAGTGCCAACTTCGTTACCCAGAGCTTTGAGGAAGGTGTTGACCATGCCCCCACCTATTAAAATATGATCCGCAATCTTGAACAGGTTATTTACTACCTGTAACTTATCAGAAACCTTAGCGCCACCCAACACAACCACAAACGGTTTGGGTGGGTTATTAATTAAGCGGTAGAGGTTGATCAATTCCCGCTCCATTAAAAGACCAATGCAGGAGTTTTTGATGTACTTGGCAATGCCAGCGTTGGAAGCGTGACGGCGGTGGGCGGTGCCAAAGGCGTCATTGACAAAGATGTCACCCAATGATGCTCAGAACTGTGCTAACTCAGCGTTATTCTTGCTTTCGTGTTTAACCACTTCACCAGCCTCATTCACATCACAGTAACGCGTGTTTTCCAACACCAAAATTTCCCCTAATGGGAGTTGATTGACTGCGACTTTGACCTTGTCACCCGTGTTTTCCGGACAAAAGTGAACCTTGGTGTTCTTTAATAAATTTTGTAAAGCACTAGCTACTGGTTGGAGTGACTTCTTACCCTTTTTGTCATCGAGCGATTTGACTCGTGACAAATGGGACAGTAACACAATTTTGCAATTGTGACCCAAGAGGTACTTAATCGTGTCTAAGCTAGCTAGTATGCGTTCGTTGTCGGTAATGACCCCGTCCTTCATCGGCACATTGAGGTCAGCACGTAAGACTACGGTTTTACCCTGAAAGTCAAACGCTTGGACTGTTTTAAAGTCAACCATGATTAAAGCTTGGCACAATAGTTAACTACCCGTACCAACTGGTTTACGTAAGAGGATTCGTTGTCATACCATGCGTAAACCTTGTAAAGCTTGTTACCATCCACTTCAATAATGTTGGTTAGCTTGGAGTCAAAGATGGAACCGTGTTCCGAGCCGACAATGTCACTGGACACAATTTCATCCTCACAGTACCTAAAGGAAGCGGAAGCCGCCTTCTTCATTGCTTGGTTAATTTGTTCTACGGTAGCATCCTTTTCAAGGGCTACACAAAGTTCAACGATCGAACCAGTTAGTACGGGAACACGAAGGGCCATACCGTTGAGCTTACCAGTAGCTTCTGGCACTACTAGGCCAATTGCTTTGGCAGCTCCAGTGGTGGTAGGTACAATGTTGCAAGCAGCAGCACGGGCACGACGAAGATCGCTGTGGGGTGCGTCTTGTAAACGTTGGTCGGCGGTGTAAGCGTGTACCGTTACCATGGTACCGTGTAAGATGCCGAAGTTCTTTTCCAAGACGTGTACCATTGGTGCTAAGCAATTGGTGGTACAGCTAGCAGCTGAGATAATTTTGTCCTCAGCGTTAATAATCTTGTGGTTCACGTTGTAAACCACGGTTTTAATTGTTTTTTGTTTAGCTGGAGCGGAAATAATCACGCGTTTAGCACCAGCTTGCAAGTGCAAACTAGCACCTTCTTCACTAACAAAGCGACCAGTGGATTCAACCACAATATCAATATTGTGTTCTGCTCATGGCAGGTTAGCAGGATCTTTTTCACTAAACACTAACACCTTCTTTTTGTCAATCATTAAGGTGTTGTCCTTAGCTACTACCTTTTTCTTAAACTCACCGTGAGCGGAGTCGTACTTTAGTAAGTGTGCTAAAGTATCGGGATGGGTGAGGTCGTTAACGGCAACGATCTCAATATTTTTTTGGCTTAAAAGTGCACGAAAAACAAGGCGTCCAATACGTCCAAATCCATTGATAGCAACACGGATAGTCTTACTCTTTGCTAGCATAATCCTCTAATTAGTTTATATTTTACTACTTTAACCTATGGCGAGTAATTCTAGTTGCCAAAAAGCTAAAAGTTGTGGACTGGAAATTTGCTTAAAAAAGCAAAAGTTTTGGTGGGTAAAGGTAAAGCTGTAGTGCGGATTGGTTCAGTACACCAAGTGTCAGTTGTTCACCATTAATAAGCAACTAAGTAAGACAAAAAGCCCCAGAAAGACTATCCCACCCCAAAGGTTGTACTTTTGGAGATAGTGCGTTTTATTAGGATTGTCCAACTGGTAACCCTTGGCTTGTAGCGCAAACGCGACTGCCTCGGTTTTTTTGACCGTTGACAATAACACCGGAATAAAGAGCGTTTTGAGTTTGACTGGATTTAAAAAGGCTAGCTTACCGTACTTAAAACCACGGATTGCTTGCGCTTGTTTAATCCGTTTTAATTCACCCTTAACAATGGGTAACAGTTTAAACACCAGTGCAAATAAGATGCTTAACGGTTGTGTTTTAATTTTGAGGTAGCGCAAAGGGGTACACAACCGTTCGACCCCAAACGCTAACTCATAAATCGAAGTGGACGCAGTTAAGAGAAAGGACGTGGCAAACAGCATGCTAATTCGTAGTGCTATTACCAATGAACGTAGTAAAGAGCGGGTATTGACACTCCACCAACTCACCCCACCAAACTGTTCACCGCCTGAATAAATAAAATGACCTAAAAAATTTTCATTTTGACTGGCCAATGCACTCGAATTCGGTAAGAAAATAAAGCCGTTGACGACAATGTTAAACCAAAAGAAGAATAACAGTCAGCCAAAGATAATGGCAAACGTTTTGAGTTTGCGCTCCACTACAATCACAAGCGCAAAGAAGATCGCATTAATAATAATTAGTCCGTGTAGCCCTAACGGGAGAAAGGCCACCACAAGGCTAATAATTCACCACCACAGCTTTAAAACGGGTGGTAACTGACTTAAAAAGTTTTTACTGGTCATGGAAGAGCGGTTTGAGTTGATCAATCAGATCGTGTACGGTTTTAATGGCTAAGGGATCGTTTAACTGCACACCCTTTGCTTGTAACAACTGAATGGTTTGCACCACAAGTGGTGGGGTAATTTCGTACCGTTGTAAAAGCTGCATGTTGTTAAAGAAATCTGCAACACTCATTTGGTCGACAATTTGACCAGCCTCTAATTGAATCAGACTGTCAGCGGTTTCGTAAATGAAATCAACGTCGTGACTGACAATCATAATGCGCGTGACCTGTTTTAATTGTTTGAGTAAAGCACTTTTAAGTGCTTTACCCTCCTGGTCTAACCCGACCGTTGGTTCGTCCAAAATGAGAAATTGCACTTGCATGGCTAATACACTCGCCAATGCCAAGCGTTTCTTTTGTCCGCCACTCAACTCCAAGGGGTTTTTAATTAAGGGAATGTGGTCCAAACCACACATCTCTAAGTACTTTTTGGCAAAGTTGACATCACCCTTTTGACACAAACCGAGGTTAATCGCTCCCGTTAACACCTCTTCTAAAACGGAATCACAGAAAAACTGTTCTTCGGCTTTTTGTAACACATAGCCAATGCTGTTTTGGAACTGCTTTGTGGCACGGTTATGTTTTTGGTTGGCAAAGTAATAAAAGTTACCACAGTTCAAAAAACCACAGGTCGGATTTTCAACTAAACCTAACTGTTTAATTAGGGTCGTTTTGCCACTGCCACTAGGACCAAAAATAACGGTAATTTGGTTAGGTTCCACACTACCGGTAACGTTTTTAATAATTTGACCAGCATTGCTTTTATCAACAAAGACACTAAGATTGTAAAAATACAGCGTACTTTGCGCTAAATCAACTAACTGCTGGTTAAACAGCGGGGTGTGTTTTTTAGCTGTTAAATAATAAAAAGGGTGCACCAGTTTATCAAACTGTTTGCAGTACCAAAAGTAGTGTTTAATGCTGCTTTGAATCAGTTTGCGGTTAACCTTATTGGCTAAACCACGGTACTGTGGTGCAATCAGTGATCAAATTATTTGAGGTGTTTGCATATTCAATCAACAACGTCTTGCAACTCCTCAATTGGACCCTTCATTTCGTGGTGGAGATGATCCGCTACTTCATGGGCCAAAAGTCATGGAAACGGGAGGGTAAAGTGGTGCTGGTGGAACAAGTGGAGCTGTTTGTATATGGCAGCGGGTGAAAACTGTTTTAACAGCTGTCCCTTACTTAAAAAGATAATTTCATCCGCTAAGAAGAGGTCATTAAAGTCATGGGTAACATTAATAACTGTTTTTTGCTGTTCTTTAATTACCTTAAAAATGAACCGCTTTAGTTTGCTAGCTAGCTTACCATCCAACATACTAAAGGCTTCATCTAGTAAATAAAGTTCGGGTTTCACCGCCAGTAAACAAGCAAACACGGCCCGTTGTTTTTCGCCAAACGACAGCTTACTGAGCGGTGTGAACTGTTTACCTTGTAGTTCTACAACCGCTAAAACATCAGCAATCACTCGGTCCATTTGTTTAGGATCCACCCCGTGGTTTTCCAAGCTAAAAATGAGCTCTTGGTGCAGCGTATCACCCAACAGTTGCATGTCGGGATCTTGCAGTAAGATACCAATTTGGCGAAAACCAACTTCTTCCAAGGGTTGACCCCTAAAAAAGATTGTTTGTTTCGGTGCTTTTAAAAATCCGCCTAACAGTTTGACCAGTGTCGATTTACCCGAACCATTGTGGCCAATAATGGCTACATGGGTGTTGGGTTTGAGGCTAAAATTGAGCTTTTTTAAGGCCGGTTGACTGTGGCGCGGATAGGTAAAACTGAGGTTGGTAACGTTGAGCAATTTAAGTTTTTATTTTTAAATTTTAAAAATAAGACTAATTGAAAAAATGGTTTAAAAAGTGCTTAAAGACGTCTATAAACCATTATTACAGGTCAAATAAGACATAAAAAAAGAGCAGTTTAACTGCCCTCTTTTTATTGATCTACTGCAACAGTAATTATTGTGCTGGTTGACCTTGATCCCCGTTAGGGTTGTACTTCTTGGCCATTTCTTCCCTTCACTTCTTGAAGTTTTCGATTTCGGCTTTTAACTTGGTGTAGTCATTGGCCTTAATTGCTGCATCAATACCACCGGTAATCTTGTCGAGTTTTTCCTTCTCTTCTTTAGGGAAGTCCTTCGCTTCGGGACTTTGGAGAATCTCCTTAATCGTGCTCACGATGCTTTCACCTTCGTTACGGAGCTCAATGCGTTCACGAATCACATTGTCACGCTCCTTGTTGGCTTCCGCATCACGAATCATCTTTTGGATTTCCTCTTCGGACAAGTTGCCGTTGTCACTAATAGTAATACTGTTTTCCTTTTGAGTGGTTAAATCTTTAGCCTTCACGTTTAAGATCCCGTTGGCGTCCAAGCTAAAGGTAATTTCAATTTGGGGTTTACCCTTGGGTGCTGGTTGGATGCCCCCTAAGTTAAAGCGACCCAAAGACTTGTTGTCACGTGCCATTGGGCGTTCCCCTTGACAAACCACCACATCCACTGATTCTTGGTTGTCTTGCGCTGTAGAGAAGATTTGACTCTTACTTACAGGAATGGTGGTGTTACGCTTAATTAACGGAGTTGCTACACCACCAAGGGTTTCAATCGAGAGCGTTAAAGGAGTAACGTCCAACAGTAACACGTCCTTTACATCACCGCGTAACACACCCCCTTGGATGGCAGCACCAATGGCTACCACCTCATCCGGGTTAATTGAGCGGTTTGGACTGTGTCCTGGTACCATTGATTCCACTAGTTTTTGCACTGCTGGCATCCGGGTCGATCCACCCACCAACAAGATTTCATTAATTTCTTCTGGTTTAATCTTGGCTTCACGTAAAACATCCGAAATTGGGTTGCGAGTACGTTCTAGTAATTGTTTGGTAATTTCTTCAAACTTAGCACGGGTTAGCTTAAGTTCAACGTTTACCGGACCCTTTTCGGTAACCGTTAAGAACGGTAAAGAGATAATTGCTTCTAGTTGGGCGGAAAGTTCAATCTTAGCACGTTCAGCCGCTTCCTTTAAGCGTTGCATAGCCATCTTGTCATTGGACAAGTTAAGCCCATTGTGTTCTTGGGCAATGTGCGCTAAGATGAACTCAATAATCTTGTTGTCCCAGTCATCACCACCCAAACGGTTGTCCCCAGCAGTGGCTAATACTTCGAAGGTACCTTCAGCAATGTCAAGTAAGGAAACGTCAAAGGTACCACCACCAAGGTCGTACACCAAGACTTTCATTTCTCGGTTAGACTTGTCGATCCCATAAGCCAATGCAGCGGCGGTAGGTTCGTTAATAATCCGCTCAACGTTTAAACCAGCAATCTTACCAGCGGTTTTAGTAGCGTTCCGTTCTGCATCGTTAAAGTAAGCAGGTACGGTAATAACAGCACGGGAAATCGTTTTACCAATCTTCTTTTCCGCATAGTCCTTGAGGTAGCTCAAGATTTGCGCTGATACCTCTTCAGGAGTTAACTCTTTGGTAGAACCATCAGGATTCTTAACGGTTACCTTATTGGAGGTACCCATTAAACGCTTAATGGAAACAATAGTATTAGGGTTAGTTACCATTTGCCGTTTCGCAGCATCACCCACAATAATTTCGTTGTTCTTGTAAGAAACAATCGAAGGGGTGGTGCGTTTACCTTCAGGGTTTTCCAACACTACTGGGCGTCCATTCTCCATGACCGACACACAGGAGTTAGTGGTACCAAGGTCAATGCCGATAATTAAGCCGTTATCTGTACTCATATGTTTATGTGCTAAATATTATAAACAAATCGTTAGCACTTTTAGCGTTTGAGTGCTAATTATGTAACTTTTTATTGTTCCAAAAGTGGTGGTTAATCAAGTTAACTGTTGGTAATACTGGTTTGGGGTGTTTGAATTCAATTACTACTTGAACCACTCAACCAATAAAACAGAGACTAAATAAGATAGCTGGAATATAAACAGAGAGAAACCTTCACAAAAAGACCCCTTGCTTTACTTGCGATTCAATACCAACAGCCGATTTAAAGGCATTTAAAAAGCTTGTCATTACAAACTGGGTAGCACCTTCCCCACTAGGTACGGGAATAAAGTTAGAAGCGGTAATGGCAATGTTAGTGATGTTGAAAATGTCAGCCGTAGAGTACTGGTCAATAACGTTGTTAGTTGCGTTTTCCTTTTTGACAATGGCAAAAACAGCAAACACACTAAAGTAACCCACCACTGCTATTAAGATATTGGCTAGTAATTTGAAGATCGTCAAGCCTAAACGTTTAATTTCTAAACCATACAACTTATTAAACTCTGCCTTATCAATAAAACGCTGGTAAATCTGTTCCTTAGTTAAGTAAGGACGCTTAATTCACTTACGAAACTGGTTTACCCCGGAGTAAATTAAGACATGCATCCTTTTACTGTAGGCAATAAAGATAAATAAGATGGCTACCACCACATCAAAGATCATCCCCGCAAAACTAAATCAGTACGACACAAACCCTTCGTGGTTTTGTTCTAACAAAGCATAGTTTTGGGAAAGAACAAAGAAGGATGGTCAAGTAATTAACGCTTGGGCAAGGTTCCAAAAGGCGCCCGTGGAAGTAACTAATAAGACCGCTGTCTGCTTCTTCATGCCCTTTTTAATGAACCAGTACAAGCGAAAGGGATCTTGACCCAAGGAAAGTGGGGTGACAATTTGAAAGAACTGCACTACACAAGCAAACAGAACCCACTCCCATCAAGGGGCATGGAAGCAAAAGCGGCGTGCTACTCATCAATTAATGATTATGTTTCAAGCTAGGGTAACCACAATCCCCAATACTATTAACAGTATTCAGGGTCAGTTAGTTCTGTTAATGGTGTTAATAATCGTTTTAACATCACCAGTTTTAATGTCTAAAAAGAAGACGGTCACCAATACACTAACAACCACCAAAAAGGCAATAAAAAAGCTAAAGGCAAGAATATTTTTTCTAGTAAAAAAACTAGCGCTTTTAGGGGTTGACATTGACCTTTGCTATTTGAGCAATTTTAACCCACTTTCTATCCGTTTATCACCAATATTTAACTGCGGCTTATTAATGGCACTAAGTGCCAACTGTTGTGTGCCCTTGTCTGCTGCTTGGAATAAAAGCAGCGCTGCCACAACATCACTGGCAATTTTATTGTTGGTACCGCTTGTAGTTGTTAGACTCAAGCTGTCAATGTCTTGGTAAGTGAACTGGGTGTAGGTGTACTTGTCACTACCATTTTGGTAAGTGAGGTAGTTGCCAGTTCCACTTGGAGTAGAATCCCTTTGGCTCTTTTGGGCACCATTACCAAAGTAGTTTTGGTACTGGTCCAAGAGGTCTAATAACAGTTTACGCTTAGCTTGGAAGTCCAAAGTTGGGTTGCCAACGGCATCCACTTGGAAGTAGGTCGCAATTGTTTTGCCACTGCCACCATTAGTTGTGGAAGGATTTAATCCACTTAACTGGACTGCAATCCGGTCTAGTAACAATTGGTTATTAGTCCCTTGAATGATCTTTTTGAGTTGATCCTTGGAACCGTACTGTTGCAAGGAGGTTTTCGTAATTTGCTGCAAAATGGCATCACGGGAACTAGCATCGAACATCGAACTAGTGCTGGTACTCAAACCAGTAAAACCATAGTGATCAGTTGAATTTTGTTGCATTTGTTGTTTTTGTACTAATGCTAAGCTAGCAACTTGTTGAATGGCTTGTACCGTTGGCTTGGTTGTTGTTTTGTAAAAGAAGTTATTGGGATCTAGTGTTTGAGTGGATTCTTTAACTTCGTTAGCGGAGTTCTCATAGTTGAAGTAGGAACCAAATACGCCTTGGGGATTAGAGAAGTTAGTGGTTTCAGTGTTCTTGTAAGCTATAAAAGCAACTTCACCAAACTTAAGGTTTGTTTTTAACAGTGACTTAAAGTTCTTTAATTTTTGGTCCACTAGCCACTGTAAGGTATAGATAAAACGGTAATGGTCAATGACCGCTTTGTAATCAACGTTAAAGGACTTTTCGTCGATGCTAGCTTCAAAAGCCAGTTTGAAAAGCTCATCAAACTTGTTGTCCTTCGGGTTACCGTAAATCGATTTGTCCGTGAGTTTAGCCCAAGTAGTGTAGTAATAGTTACCGATAACTTTACTTAATGCATCGGCATCACTGTTGGTTGTGGAACTACTTTGCATTCCACCATTATTACTGGACTTTTCCTGAATGTCTTTAAACAACTTTTGTGCTTTCTTAAATAAATCGTTATTATCGAACGCTTGTTTGAGCAACATAAACGAAAGCGGGTTGACTTTGTCGCTCTGTAATAAGCTAGCTAAGCTCCAGTTAGCACCACTAGAAAGTACTTTGTCATTGTTAACGTTTAACAAAATGACGTTACTGTAAGCTGGTCGGGGTGCTAAAGCAGGTTTGAGTTTGTTTTTAACAAAATCATTTACTTTAGTTTGGATGTTTTCCAAGATTTTGTCCCTAATCTTGTCAACGTCAGCAGTTTTAGTTTTGAGCTTATTGCTTTCTTCACTTATGCTTGTTTCTTCTTGACCAACTTTAGGTCACAATGTTTTTGTGATTAAGAAGGTAAAGTAGTTGTTTAACTTTTCGTAATTACCGTTAGTATTAACGTAAGGTAACTTGGCTGACAAACCGTTCAACCAAGGGGTTGGATTGGCTTGGTTGACGTTTTGGTTAAGATTACGGGCGCGCTCTAGCAACTTGTTATTGAGCGCTTTCATTTGATCAAAATAAAAGCTGATCATTGTGGTTGCTAAAGCCACCGGCGCAAATTCTTTAATTAACTCTTTATAAGGATTAAAAGCTTCAGTTAAAGCTTTGCTAGCTTTATCCTTTTGGTCAAGACTAGCGTTAACTAAGGCGTTGGCCAAAATTAGTTCCCGGTGTTTATCAAACCAACTTTTTAGCGTTTCGTTTAAGGGAAAACTAAAGGCAAAGTTAGTACTGTAGAAAGTGTTAGTTTTAGCTTGAAAGGCACGAAACTTTAAGAATTGTTTTTGCTTGTCAAGGTCTCTTAATACTTTATTACTGTTACCTGAACTAGATTTAGTTAATAAGGTTAAACTTTGCTTAGAAACTGATTTTTTATTTGTTGAATCTTGTTTTAAATAATAGCTCCCACCATCAAGGCCGAAAACAGTTACTCCGGTGCTGTTACGCACAATCCGGAATTTAATGGGACTGTAACTGTTTTGATCAGTTTGCTGAACCTGCCTTAAATTTATAGATCCAGATTTTAAGGCAACTAAAACTTCATTAGAAGCGCTATTATTTTTCGATTTTGATGTTGTTGAATTAAATGTAGGAATAGCTGCATCTAAAACAACGTAATTGTTTTGTTGGCCCTTAGAATTGTTAGTTCTTAAAACAGCTGTTTTCGCTTCCATTTTTGTTGCAGCTGCTTTCTTTACCTGCTCCGCTAACTTACTTAAGTTAGTGCCTTCGCTTGTGTTTTGAGCAAAAATGTCATCTAAAGCGCCAGCAATTGGATCTGAGTCACTTTGTTGTTGCTTACCTTCTTGATCATTGTAAAACAGTGCGTTAGTTTTGGAAGCTTTTTCTACGTCCTTACTTTTATCGACAACAATCGTGTTGTTAAGGTCTTCTTTGAGCTCCTTAATTTGCGGCAATTCGTCATTATCTTGCTCAAAAACTTGCAGTTTTAAATTTAAAGCTGCACCTAAGTTAGCAGCTTCTAACTGATCGACAATGTTAGAAGCGTCTACTAATTGCTTCGTTTCGTTAGTATCTGATAAATTTAAGGGAAAATCAAGAAAATCACCCTTATTTGATTCATTACGGTATTTGAGCAGACCAGTTTTTTGGCTGTTTGTACCGCTTTGACCGATATTATTATCAGTAATAGCGCCCTCACCTTTAACTAACTGCTTTCAGCGCTTATTACCAACGTTTTGGTTGGGTGCAATGTCTTTTTCTTCGGCAAAGAAAGGAAAGGCATAAGAAGGTGTTAGTTTATCCTTTAACTTCTCTCTGTTATAGATCTGACCTAACCCTTGACTGGGAGCAGAATACTTATAGTTAACTTGACTAATTAAAGTGGGATCAGTGTACTCCACCCATTGGTCAAATACTTGGCTTTGAATTTTGGCGAAAAAGTCATCATTAGTTTCACTAAAACGTGGGGCACTAAATTCAAAGTTTTTTCAGCTTTCTTCCTTGTGTAGTTCATCATAAGTAAATGGACCAGTAGAAACTTGTCCATCTTTTTTGTAATTGAGGTAGTTCTTTTGGAAAACCCGACTAGCAAAGTCACTAATGAGCTGTTCAAGCAGCTTTTGCGCTTTCCAGGTTGCTTCACTACCACCGGCGTTATCCAACAAATCTTGTTGCACAAAAAGTGATTTATTGCGTGATGCTGATACCACTTTATCGACTGCCGTTGTGTACTGACTGTCAACATTGGACTTAGTGTCTTTTAAAAACTGGGTAATCTTTTTGTCTTCGTTGTTTTCGTACCAAGCTTCTAGTGCTTTTAAAAGTGGTGCAGCGATAAATTGTTTGTTGGCCACTGGATTTTTTAAAGCCGTTGTTAGGGCACTTTGTAAACTACCCTGTTCACCATTGAAAAACTGACTGGAACGCGCTTTAAAAACGGTTTGTAAAGCCGAGTTGGAAGGGGTAGCACAAGCAGCTAAAATAACACTAGTAGACAGAAAACCGCCTAATGTTAAGAGTCAAAATTGGGGTTTTCTTAGAAACTTTTTCATTAACGCGGTTTTTGCTCTCTAAAGGTTAAATTTTTGCTTTATTTTGGCACTTTAAAAGCTAGTTTTTACCTAAAAATAAGTATTGATAAGTAAGAAAAACTGCTTTAGTGCCCACTGGTGCAACTAGCTTAATCTCTTGGCTTAATTTTGTACATTTTAAACCACGATGGCATGACAAACTTACAATTTCAAACGTTTTTTTTTTTTTTTTTGAAAGAAATTGATTGCTTTAACTAACCTGCTTTTATCTAACTATTTAAGTAAATCTATTCCTGTTTTAATCCGCTTATCACCAATTGGTAGTTGTGGTTAATTAATAGCACTCAAGGCTAATTGTTGGGTACCTTTATCCGCTGCTTGGAAAAGTAAAAGGGCAGCCACAACATCGCTAGCAAGCTTACTATTTCCTTTATCACTCAAACTTAAGTTATCTATATCTTTATAGGAGAACTGAAGGTAAGTGATCTTGTCACTGCCATCTTGGTAAGTTGAATAGCTTCCATGTCCACCTTTACCAGAATCTCTTTGTGCAGCCTGAGTATTTGTACCAAAGAAATCTTTGTACTGATCTAAAACATCTAATAAAAGTTTTCGTTTTTGTTGAAAGCTAAGGGTTGGGTTGCCAATGGCATCTAATTGGAAATAAGTAGCAATATTTTTACCCTTTCCATTATTACTATTTCCAGTGGTTGAAGGATTTAATCCACTTAATTGACTAGCGATTCGGTCTAATAAAAGTTGGTTATTAGTCTTTTCAATCAAAGTTTTCAGTTCTTGCTGCGAACCATATTGTTGCAAGGAATTCTTGGTTATTTGATCCAAAATGGCTTGCTGCGTAGCGGCACCTAACATTGAACTAGTACTGGTGTTTAGCCCGGTAAAACCATAATGATTACTATCTTGCATGGTACTTTGCATTTTTTGTTGCATAACCATCATGCTTGCACCATTTTGTGCGGTTTTAACTTCTTCTGGTTTGGTAGTTGTTTTATAAAAGAAATTATTTGGGTCTAAGTTTTGCGCAGCAATCTTAACTTCATTAGTTTCGTTTTCGTAATTAAAAGCAGAACCAAATACACCTTGCGGATTGGAAAATTCTTTGGTTTCAGTGTTTTTATAAGCAATGAAAGCAATTTCACCAAACTTTAAATTTGCTTTTAATAAGTCCTTAAAGTTCTTTAAGTTACCGTTAACTAGTCATTCCAAGGTATAGATAAAGCGGTAGTGTTCAATGACCGCTTTGTAATCAACATTAAAGGACTTTTCGTTAATACTATCTTCAAAAGCTAATTTAAATAAGTCATCAAATTTGTTGTCCTTAGGATTTCCATAAATGGATTTATCAGTCAATTTAGCTCAACTGTTGTAGTAATAGTTGCCAATTACTTTACTCAAGGCATCAGCTCCTTCACTACTCGAGGTATTGCTACCTTGCATCCCACCTGAACTTCCACCATTTGTTTTACTTTGAATATCTTCAAAAAGTTTCTTAGCTTTCTTAAACAAGTCGTTTTGGTCAAACGTTTGTTTGAGCAACATAAATGAAAGTGGATTAATTTTGCTCGTATCCAATAAGTTAGCTAAAGCTCAATTGGCGCCCATCGATCAAACCTTATCGTTGTTAACGTTTAACAAAATTACATTACTGTAAGTTTGTCGTGGTGCTAAGGTTGGTTTAAGCTTGTTTTTAACAAATTCTTGAACCTTCTTTTGAATGTCTTCTAAGATTTTATCTCTAATTTTGTTAACGTCTTCGGTCTTAGTTTTGAGCTTGTTAATGCTACTATCTTTAGATAATGAAGCGTCTTCATTACCGACCTTCTTCCACATGTATTTAACAATTAAGAAAGTGAAGTAATTGTTGAGTTTTTCGTAATTACCATTTGTTCTAACATAAGGCAATTTAGCTGATAAACCATTTAACCAAGGTGTAGGATTGGCCTGATTCACATTTTGGTTTAATGCTTTAGCGCGCTCCAAAAACTTATTGTTCAACGCGTTCATTTGGTTAAACAAGAAGTTAATCATGGTTGTGGCCAAACCAATTGGTGCATATTCTGCCAGTAAATCCTTGTAAGGCTTTAAGGCTTCAGTTAAAGCTTTGTTTGCTTTATCCTTTTGATCAAAATTTGCCTTTACCAAAGCGTTCGCTAAGATTAGTTCGCGGTGTTTATCAAATCAACCTTTCAAGGTTTCATTTAACGGAAACGAAAAGGCATATTTGGTGCCGCCTTGTTTGAAAACTCTAATTTTTAGCTTGAAAGGCACGAAACTTCAATAAATTTTTTTGAAGACTGCTATTAATCTCTTGTTCTTGATTATTATTACTTTGGTTTTGAGCGAGAGAAGTGGCTATCGTAGAAGAGCTACTTTGTTGTTTATTGGAAGATCCAACAACGTAGTAAAGCAAGCCTTCAAGAGCCAGCAATTTTACACCAGTACTATTTCGAACAATGCGCTGGTTTAAAGGCGTTGTACTACTTTTTTGTTGAGTTAAATTGACGCTAGTAGAAGTCAAATTAACTAAAGCGCCACTACCAGAAATAGTTCCACTGCTTTGTGGTGATTGTGAAGAAGTTTCAAACGCCGGGATCGCAGCATCTAATAACAAATAGTTTTCATCAGATTGTGCCATGTTTGTGGTTTTGAGACTAACCGCATTTCTTAGTTGTCATGTCTGACTTCTAGCTTCCATTTTAGTACTGTCAGTTTTTTGTAACTGTTCAGCTAGCTTACCTAAATTACCACTATCGCCATTTTGACCATTAAAAATTTCCTTTAATTGAGTTTTAGGTGAATTTTTGCTTTCAGCACTTCCTTGTTTTTCGCTACTATCACCATTATCTTTGTAAAAAGTACTGTTAGTTTTAGAAGTTTTCTCTAAGTTATCAGATTTAGCTACAATGGTGTCATTCAAATTGTCAGGAAGCTTTTTAATAACAGGCAGTTCATCAAAGTCCTGTTTTAAAAAACTGAGTTTAAGGTTAAGTGCTGCTCCTAAATCGGCTGCTTCTAGTTGATCAACAATAGTAGTGGCATCAACTATTTGTTTAGTTTCACCAACATCAGTAAGAGTATTCGGGAAATCAAATAAGTTTCCGCTTGATGTATCACCTCTTCATCCAAATAACCCTTTTGTAGAAGAGGAACTATCGTCGTCATCTGAACTCTTCTCCTTGCCATCATCAGAGCCCTCGCCTTTAATTAATTTCTTTCAGCGCTTGTTACCAACGTTTTGATTTTCCGGAATATCTTTTTCCTCAGCAAAAAAAGGGGAAGGCATAGGAAGGAGTAAGTTTATCCTTTAACTTATCTTTGTTATAAATTTGACCTAAACCTTAATTAGGAGCTGAATACTTTAAGGTAACTGAGCTAATAATAGTTGGATCGGTGTACTCAGCCCATTGATCAAAAACTTCGCTTTGAATCTTCGCGAAAAAATCATCATTAGTTTCTGGAAAATTAACAGCATCAAAAACTATATTCTGTCAATTACTATTTTTTGAAATCGTGTCGTATAAAAACGGACCGGCTGATACTTTGCCATTTTCCTTATAAGACAAATAATTCTTTTGAAAAACTCGTGAAGCAAAATCACTAATTAGCTGTTCAAACAGTTTGCGCGCTTTTCAAGTGGCCTCACTACCACCGCTACTGTCCAATAAATCTTGTTGTACAAATAAGGATTTATTGCGTGGTGCTGAAACTACTTTATCTACAACGGTTTTGTATTGGTTATCAACATTAGTCTTGGTGTCTTTTAAGAATTGAGTAATGTTTTTATCTTGATTATTCTCGTACCAAGCTTCTAATGCTTTTAAAAGTGGAGCAGCTACAAATTTTTTGTTAGTTTCCGGATCTCTTAAAGCGGTGTTTAAAGCGCTTTGAATGGTACCATGCTCACCGTTAAAAAATTGGTTGGAAGTTGGTTTAAAAACTGCTTGCAGTGCTGAGTTTGAAGGTGTTGCACAGGCACTGAGAATAATACTACTAGTGGAAAGGCCACCCAACAATAAAAGTCAAAACTTGGGTTTTCTTAAAAAACTCTTCATTTAACAATAGTGCCGTTTGTTCTAAAAAAGAAAAAAGCAAGCTTACTTTTTGGCTTAAGGTTTTTAAATTTTAAAGCACCTCGATATTTTTGAAAAATTATTTCTAAACTTTTTTTGCAAAAAGTTGTTAGGCTTAAAACCAATCTTTTAGCTCACTATTTCAGTAAATCGATCCCTGTTTTTATGCGTTTATCGCCAATTGGTAATTGTGGTTTATTGATGGAACTTAAAGCCAGTTGTTGAGTACCTTTATAGGCTGCCTGGAATAATAAAAGCGCTGCTACAACATCACTGGCAAATTTGCTGCTAGTTCCGTTTGAACTACTCAAACTTAAACCGTCAATATCTTTATAGGAAAACTGCAAATAAGTGATCTTATCACTACCGTCGGTATAGGTTAAATAGTTGCCAGTTCCACTCTTACCAGAATCTCTTTGAACTGCTTGTGCATTATTACCAAAGAAATCTTTGTATTGATCTAAAACATCCAATAAGAGTTTTTGCTTCGCTTTAAAGTCCAAAGTAGGATTGCCAACCGCATCTGTTTGGAAGTATGCAGCAATTGTTTTTTGGGTACCACTGTTGCCACTTGTATTCGGTTTTAAGGCTATTAGTTGGTTGGCAATTCGGTCTAATAATAATTGATTTTTAGTTTCGCCAATGATCTTTTTCAGGTCAGCTTGAGAACCATACTGTTGCAAGGAAGTTTTGGTTATTTGATCCAAAATGGCTTGCTGGGTAGCAGCACCTAACATCGAACCACTACTGGTACTCAAACCAGTAAAGCCATAACTGTTAGTTTGCTGATTGTTCATCTGCGTATTTTGAACCATCACAGCTGTATTAGCACTTTGAGTGGTTTTCGCTTCTGGTTTAGTGGTGGTTTTATAGAAGAAACTGTTGGGGTCTATAATTTGCGTAGCACTCTTAGCCGCATTCGTTTCGTTTTCGTAATTAAAGTAAGAACCGAATATACCTTGCGGGTTAGAGAAGTTCTGCGTTTCAGTATTCTTGTAAGCAATAAAAGCAATTTCACCAAACTTGAGGTTTGCCTTTAATAAATCCTTGAAGTTCTTTAGGTTTTTGTCTACCAACCACTCTAAGGTATAGATAAAGCGGTAGTGTTCAATCACTGCCTTATAATCTACATTAAAGGACTTTTCGTTAATACTACTGTCAAAAGCCAATTTAAAGAGATCATCAAATTTGTCATCCTTAAGGTTGCCATAAATTGATTTATCGGTTAGCTTAGCTCAAGTGTTGTAGTAATAGTTGCCAATGACTTTGCTCAAAGCATCGGCATCATCGTTGGTGGTTGTACCACTTTTACCACTTCCACTAGTTTTTGCTTTATTTTGAATGTTTTCGAATAAAGTCTTTGCTTTTTTAAACAAACTGTTTTGATCAAACATTTGTTTGAGCAACATAAACGAAAGCGGGTTAACCTTTTTCGGATCTAGTAAAACTGCCAAACTTCAATTAGCGCCAGCAGACCACACCTTATCATTGTTAATGTTTAACAAGATTACGTTACTGTAAGCTGGTCGGGGTGCTAAGGTAGGTTTGAGTTTTTGGACAAAAGTTTTGAGCTTACTGTCGATGTTGTTCAAAATCTTTTCCCTAATCTTGTTAACATCTTCAGTTTTAGTTTTGAGTTTGCTTTTTTCTTCACTGACATTAGTATCCTCAGTGCCCACCTTAGGTCACAAAACTTTGGTAATTAAAAAGGTAAAGTAGTTGTTTAACTTTTCGTAATTACCGTTAGTGTTAACGTAAGGTAGTTTAGCCGATAACCCATTTAACCAAGGGGTAGGATTGGCTTGGTTAACATTTTGGTTTAAGCTCCGTACTCGTTCTAACAGCTTATTGTTGAGAGCTTTCATTTGGTCAAAATAAAAGCTAATCATGGTGGTAGCTAAACCAACCGGGGTGTATTCACTCAATCAGTTTTTATAAGAACTAAAAGCATCCACTAAGGCCTTACTTGCTTTTTCCTTTGTGTCTAAAGTAACTTTAACTAAGGCGTTCGCTAAGATCAATCCGCGGTGTTTGTCAAACCAACCTTTTAAAGTTTCGTTTAGGGGAAAACTAAAGGCAAAGTTAGTGTTATAAAATGTGCCAATTTTGGCTTGAAAAGCACGGAACTTTAAGAATTTCTTTTGCTCTTCTTTATCAATCTTTTTGGCACTACTAGAACCGCCATTGCTACCTCCGCTACCGCCTTTTATGAATTTAACAAAACCATCAAGTCCTAAAACTGATACGCCAACACTGTTACGCACAAGTCTCAGGCCAAAAGTGTTATATTGATCTTTAGTTTCTCCTTGCTGTTGCATTTTTAGTTAGGCAACTGTAGATGTGTTTAAAGAAATTAAGGTACCGTTATGAAGACTCACTGAACCTTGACCCTGGGTGCTGACTTGCTGTTTAAAGGTAGGAATACCAGCATCTAATAGAACATAACTGTTCTTTTGTTGAGTTTGCGATTTATTTCGCAAAACAACAGTGCCTCTTGCTTGTGGGGTTTGCGTTTTAATTTCCGCTGCCTTTTTAATTTGTTCTGCTAACTTGCCTAGATTAGTTTTTTGTTCCACTTTTGTGGCAAAGATGTCATCTAAAGCTTGGGCAATGGGATCGGTATTTGTGGTTCCAGAGTTTTGATTATCCTCATAAAAAGAAGAATTAGTTTTGCTCACATTTTGTGCATCACTACTCTTATCAATCACAATCACGTTGTTAAGGTCATCTTTGAGCTCCTTAAATTCGAGTAGCTCATCCTTCTCTTGACCCAGTCGGTCTAGTTTAATGTTGAGCACAGCGGCTAAATCAGCTGCCTCTAATTGATCGACAATGTTGGTGGCATCCACGACTTTTTTGGTCTCGTTAGTATCTGATAAGCTTAACGGAAAGTCAATTACTTGGCCTCTGTCGGTTCCCCGTACATACTTGGTAAGGCCTTCTTTTTGAACTCCTTTTGTTTGACTGCCATCTTGTTGTTGCTTTGGCTTTTCTTCAATCGCTTCTTCACCTTTAACCAGTTGTTTTCAGCGTTTATTGCCGACATTTTGACTCCCAGCAATTTCATCCGCTGCAGCAAAAAACGGAAAGGCATAGGAAGGGGTTAATTTATCCTTTAGTTTGTCCTGGTTATAGATTTGCCCTAATCCCTGGTTAGGAGCCGAGTACTTTAAGGTAGTGAAACTAATAACAGTGGGATCGGTGTATTCCGCCCATTGATCGAAAACTTCGCTTTGAATCTGGGCAAAGAAGGCATCATTGATTTCGGGAAACTGGCCCGCATCAAAGACAATTTTGTCCCAATTGCTCGATTTAGAAATTTCGTCAAATAAGAAAGGACCTGATGATACCTTATTACCATCCTTATAAGCGAGGTAGTTCTTTTGAAAGACCCGACTAGCAAAATCGTTAATGAGCTGTTCAAACAACTTCTGGGCTTTTCAAGTTTCTAACCCCCCCGGCGCTATCCAGCAAGTCTTGTTGGACAAAGAGGTTTTTGTTTCGGCTAGCCGATACCACCTTATCTACGGCTTGGTTGTATTGCTCATCAACACTCTTTTTGGTGTCTTTAAAGAACTGGGTGACCTGTTTGTCTTGGTTATTTTCATACCAAGCTGTCAATGCCTTTAAAAGGGGAGCAGCGACAAACTGTTTGTTGGCCTCTGGATCTTTTAAAGCAGTGGCTAAAGCGTTTTGTAAACTACCCTGTTCCCCGTTGAAGAACTGGTTCGAACGTGCTTTAAAAACAGTTTGGAGTGCTGAATTGGAAGGGGTAGCACAGGCAACTAAAATAACACTTGAAGAGATAAAGCCACCAAGGGTTAAGAATCAAAACTGGGGTTTTATTAGAAGTTTTTTCATGAACGGCGTTGTTTGCGCTTGGCAAAGAGTTCCGCAATCTGCTGCTTTTGGCGCTTCTTATAGTTAGGCTTGACCTTCTTGTATCCTGCCTTAATCCGCTTAATGGCAGTTTTAAGTTCACTGGATACTGCTGGTTTTTGTTTGCTTTGTTTAAGTTGGGGGAACTTTAAAGTACCATCACGTTCTAAATGAACACTGTTAAACAAAATACCTTTATGGGCAAACTTAGTTAATTTGTGTTGGTTATGCGGTAAATCAAACACATAGGAATTGCCCCAACTGTTAAAACGCGCTACTCTACCAGAACGGTGGATGTAAAAGCTATCCACTAGGGGTAAGTCTCATGAAATGACCACACTAAAGTGGGGCAGATCAATGCCGCGTGCTAGTAAGTCAGATACCACTAGTACCCTCACCGTGTTATTAGCGGCACTAGTAAAGATGTGTTTGCGCTCATTGGGCGTTAGTTGACCATGGAGGCTTTTAAAGCTAAGTTGGTTGTGGGCTAGTAAACTAGTGAGTTGTTTGAGTGATTTAGCGTTGGAACAAAAGACCAAGACCTGTTCGTTATGGTGGTAGTTGAGTAAAGCTAATAAACCAGCAAACGGTTCTTGACCGTTGAGGTTAAGCAGAAAGTGTTTCACCAAAGAGTGCTTTCAAGCACGTTTCTTTTGGTTGACGACCGTTAAAGGTGCGACTTGCTTGCGAATTTGCTGGAGCTGTTCGTTAAATAACGTGGCACTAAAAAAGCCAAAGTGAGGTTTATGTTTTTGAAACAGTTGGACACAGTTAATCCACTCTGCTTGGGAACTGTCCTCCAAAAACATGTCGACCTCATCAAAGACAACGTACTTAATCGTTTCGTGGAGAATAATGTCTTTGCCAATTAAGCTCACAATCAGGTGGGCATTACTGTTGAAGTTCTCACTGACCTTTAAACCAGTAAAGCTGGCTTTAATGTCGTTGAGGATCGTTAAAATTTGCCACTTCAACTCCTTAGTTGGTACAAACACCACCGCTTGGGGCTTGTTGAGCGTGACATCAATTTGGTTGAGCGCTGGTAAGAGGTAAGCGAATGTTTTACCACTACCAGTTTCGGCTATGCCAACCACATTTTGACGTGGTCAAACTTTAAACACAGCTTGTTGAATAGATGAAAATTCAACAATGTGCTTCGTTTGTAAGAACGCTTGGAGTGGTTGAATGAAGTGCATAGTGGTTTAAAAACGTCTTACCCATTGCGCACTAATAGAACTAAAGATCCTAAAGTCACCTACTTTAACTAAACCCTTCTTATTACCACTAATTAAGGCGTTAATGGCTTGGGTTTGATTATTAGGATCCAAGGCTTCTAGCGCAATTGCAGCGAGGAATTCTTCGAGGGAGAGGTTGAGGCGTTTGTCTTTGCTGGAATCTTCTTGCTTTAGAGAATCAGTGCCGATCTTCTCCACATCATCAAGGTTGACCTGTTTGACAAAGGTAGCGTGGTATTTGCCACCGTCACTTAAGAATTGGTAGGAAGTGTAGTTCTTTTCCTCTACCTTGTTATCACCAATGTAACCATCAAAGCGGCTAAAGACGTCTTTATAGTTGGTATTTTGGTTATCTTCTTGTGTTTTCTTAATTTGTACTGTTGCAGATCTTGCACTGCGTGCACTTGTATCGCCTCTCTGTGCAGATTGAGTACTCTGCTTAAAGTTAGAATCAACAAAACTCGTTAAACTAGTTTTCTGTTCCGTTAAAGTTTTGTTTTTATCAACCCCAGTAACATCAATGTTGAGGTCACTAGTTAAGTGGTTGTAAAGCGCATCGAACTCGGTTTGCGTTTGGATTCCGTTGATGATGTTCTTTAAGTTATCCATCGAACCAAAGCTAAACAAGGCACCTTTGTAAGCGTTGTTGGTGGTGGATTCGGCGTCGTCAGCTTGTTGTTTTAAAACTAAGGTAGCTTTACCACTTTTAGCTGTGGTAATTAGCATTTGACCACCAACTTTAACGCCTTTTTCTGTTTGGTTAACAAACTGCTTAAACAAACGTCTACTTACCGCTTCGGGTAAACTACCACTAGTGCTACTCGTTACAATACCCCTAAAGCCATAGCGGTTTTTGGCAGTTTTTGTGGTCATGCCACTAGCTTGATTTAAACTCCTTAAAACAGTGGCACTACTTTGTGCTGCGCCAGTACTACTACTACTAGACTTAGTGTAGAAGAAGCTTGAAGTGTCATCAGCAGCCCGGTTCACTTCTGGGGTTTTACTGTTGGTTCAATCATACACACTCGAACCAAACACAAAGTTCGGGTTTTTGACTTCGGTTTGGGTGATGTCACTATTATTATCACCGCCTTGGCCAGCTGCTTGATTTTGTGCTAGGGAAACGACTGATCTTAACTGAATTAAGCCAGTATTACTTGTGCTTGAAGCGGTACTGGATAAAGTTGATTGTGAATCATTTTTATTCTTTTCGCTATCCACACTTCAAACTAAAAAGGCATTGGAAGTACGCGACAACTTCGACTGTAAGCTTTGCTTATAGTTCTTTAAGTTGTCCTTTAACAACCAATGGGCCGTGTAGAGGTAAGACAGATAATCAATTACATCATCACTATCTAAGCGCTCTTCTAAGTTTAGCGAGCTGTACAGTGTGTCCAAGACACCTTCGATGCCCGTCTTAGTTGAAGAATTTTTATCAGATGAACCAACATGACCAAAGATTGATTTTTGGGCTTGTGATTCAAATAAGTCCAATAAATAGGTTTCTTTAAAAATCTTTCAGTTGTCAGCGGTGAGGTTGTTGTTACCGTTGGTGCCACCAGTACCACCACTTTGTTGACTAAAAGTTAAATTCGTAATATCAATAATCTTCTTAAACGCTTCATCTTCCTGGAAGTAACTGAGCTTAATGCTGTTTTGGAGCTCAGTACTGGTTAAATAGTTGTTTAGAGCTAAGTTAAGTCCTAAAGAGGTACTTTCAGGGGTAAAAGACAACTTAGTTTGCACTAAGATTACCTGGGAGTAGAGTGGGGAAGGAATGGTGAAGACTTTGAGTGCCTCTACATGTTTTTTAGCAGCTTCTTCTACCTTCTTCTTATTATCTTTAAGCTTGCTAACTACTTCTTCAGAGAAGTTTTTAGTTTGTCCTGTATTGGAAGAGGTTGTTTTAACAGTACTAGTTCCTTTTTTATCAACATTATCAATAATGTCCTTGTAATAGTTACCAAGGTCATTATAGTGACCGGTTTTAGCATCTTGTTCGTAAGGTAACTTAGCTGCCAAACCGTTCTGTTCAATCTGGTTATTGCGCTCGTTGTCAATAAAAGGTTGGTTACGTTCTGCTAGCTTTAAGGTAACCTTGTTTTCCGCCACTGCAGTTTCGTTAAAGACTGCTTGTTTATAGTGGGCTTGAACAAGTTCTTTTAAATCCTTCTCAATTGACTTAATACTGTCAGCAAACTTCTTAAACTGGGGATAGCTTAAGAAGTTGTCTTTGTTGTTTGTATCACTAGAAAGATCTAATAAAGCTTCAAATAAGAATAAGATCCGGTTAGTGTCAAAGTACTTTTTGACCTCATCAAATAACTTAAAGTCGTATGTGGTGTCGGTATCCACTAAACCGTACTTGGTTTGCAAAGCCCGGAACAATAAGAATTTCTTTTGTTTAGCGACATCACGACCACTTTCAGTAAGATAATGACTACCACCATCCACGGCCATTAAGTGGATGCCGTCTTTACCACGGGAGAGGATGTAGTCTGGTTGACTACTAGAATCTTTTTTAACTTCAATTAAGTCAACAACCGCCTTACTACTGTTGCCAGTAGTAGTTTCAGTAGAAGATCCGGTGCTACTGCCGGTAGAGGTGCCACCGCCGCCTCCAGAACCTCCGCCACCTGTTTGATTAGCATTAGTATTACCTATTTGTTGTTTATACTCACCAGCAAAGACATCTTTATCACCAAAGATTTTGACTAAGTCAGTTTTGTGGACGGTACTTTGTGTAGTTCCACCACCATCACTGGCAGTTTTTAATTGAATAGTTCCTTCAGATGATGAACTCGCAGCCCTTGCAGCGGCGGCTGCCTGTGTATTATTGTCAACAAAAAAGTTTTCAATAATACTCTTATCCTTAATGAGACTGTCAACTTCTTTACTATCAGCGCCCTTCGACTTTTTCTGCAGTGCTAAATAGCCCTGAATGAAAGCAGCAGAAAAACTCGGATCAAAGGTGTCGAACATGTCGCTCGCCTTTAACAACAATTTGCCACCACTATCACTGGAAAACTTATTGGGAATATCAATCCCTTTGCTCTGGTCGTTTTTGTAACTCTTGAGGTTGCTAGCCAATAAGTTAAAGCCATTCGCTCCCTTAGTGCCACCACTCTGTTGGTTGTATTTGTTAAAGACCTGAAACTGGTAGGAAGGGGTTGGACTTTGAATTACTTTAGTGTTAAAGTAATTATCAAACCCGTCAACGGGAGTTTCATTGGTAAACACGACCCGGGATACCAAGTTGGGGTTTTCATTGGTAATAAACTGGTCTAACAGTTTGTCTTGGATGGCCGCGTACACCGCATCATTGTTAATCTTGAGCTTAGCATTCTTATTAACAAATTTGGGTTGGAGCTTAATGTTTTTAAAGTTAGAGGTGTTTTCAATTAAGCTCTTGGTGGGTTTAGTTAAAGGACCTACACCGTCCTGGACATACTCGACAAAGTTCTTGGCAAAGATCCGTGAGATAAAGTCATCAATTATCTTGTTGTTAACATCACGCAGTTTTCAAGCGGCTTGATTACCGCCAGTGTTGTCAAAGATATCAGTTTGCAGGTGCACTAAATAGTTTTCGCGAAACTGGTTCCGTAGGTTTTGTTCTTGACTGACAAACGCATTATCCGTGTCAGCGGTAAAGCCGTTTAAACGCTCTTTAATATCAGTATCATAATTGTCCTCATAAAAGGTGCGCAAAACGGGTGCTAAACGCATTGCTAGATACTGGTTAAAACTCTCGCGGTTAATTAGTGACTTTTGCAGAATCGAGCTAAGACTAAAGTCAGTGCGATCAGCAAAAGCACTACTGGGCTTAAAAAGCCCCTCCACAGTGCTGGCTGTAGGCTGCGCACAGGCAACCGCGACTATACCAAAAGTGAGCCCGATTGCGGTGAACATAAAGGGCCACTTGACTTTACGCGTAAACGTATGCATTCGCGTTCAAAATTTGGTATAATTCTAAAATTTAACCACTTTCTTTGACCGGGGATGCGGCTCGAAATTGAAAACGGCTTGGAATTTGTGTGTGATCCCTTTTTAAATGAGCGCGGCAAAATCTTTTTTTTACACGCCTTTACCGGTAATATCACCAATAAATTAAGCTTTCGCACCCACTTTAAAGATTACAGTTTTTACGGGATTAACTTTCCCGGACACGGTAACAGTGTCATTCACAACCAAAGTGAATTAGACTTTAACTTCTGGATTAAGTTAGTGCAACAGTTCTTTAATAAATACCAATTAAAGAACGTGGTTTTATTTGGTCACTCGATTGGTGGCGGACTAGCGATTGCCCTCACCCAAGTTTTAACTAAGGAACAAATTAAAGGCATTATCCTCGAAGCGCCCCTAAACCCGGGGATTCGCGCTACCCCACCAAGTATTATTAGTGCCTTGGTACCTGACACCAACGAAGACTTTGAAGCGGTACAAAGGGCATTGATCTACAACATTGAACAACGCTTTGGGGCGAACTTTAAGGACTTTTGTGCTAAACAAAAACAGAAGATGATCCAAAAGTATGCACCCTTAAAGGTAATGTTGCAACCCGAACAAGCCGAACAACGGCTCCAGTTAATTGATGCGGCTTTTAAACGGTTAAGTTACCCAACCCTGTGAATTCACGGGCAGGAAGATGGCATTGTGCGTTACTTGCCCTCGAAGGCTTACCTGGAATCCTTGCACAATCCTTTAATTGAACTAGTGGGCTTATCCAACACGGCCCACACCACTTTCTTTGAGCAGCCCCAGCAGTTTCTCCAACTAGTGGAACAGTTTTTAAACAAGTTAAACAAATAAAAAACCTAACAACTTTAGTTGCTAGATCTCTTATTGTTGATTGATGTTTAAGCGTAGTTACTACTTAACCAAACGCTTGTACCATTCGACCACGTACGATTCGTTAATACCAGCAGGGAGTTCACTACGTTCTGGGAAGCGCACGTATGTCCCTTCAAAGGTCTTGTTGTTGGTTTCCACAAACGGGGAGACCACACCGCTTTCGCTCGCCGCCTTAACAATTGGAATCTTAATTGTTTTGGCCTTCAAGCGTACCTTATCCCCTGGATTGAGAATAATGGAAGGGGTGTCCACGGTACGATCGTTGAGTAATACGTGGCCGTGGTTAACTAATTGGCGCGCACTGCGACGGGTTGGAGCAAAACCCATGCGGTACACAATGTTGTCTAAACGTGACTCTAAAACACGGAACAGGTTAACCGCTAAGTTACCCCGTTGTTTTAATACCAGTCTAAACAAGCGTCTAAACTGTTTATCAGTAATCCCATACATGTATTGCATCCGTTGCTTTTCTTGCAACTGTTGTGCATAACCAGACATAGTGGAACTACGGAAACGGTTCCCGTGTTGTCCAGGAATCGTTTTCCGCTTCTTCCCTTTCGAAAACTCCTTGTTGTTTTCTAGTAACGAAAAACCTAAGCGGCGTGATCGTTTGAAAATACTACCAGTGTATTTCATGTGGTGACAATTTTAAATTATCTTAATAATCAAGATTAAAATCCCCACCGTTTTCCATTAGTTCATCATCCAAGATACTAGAGAGGTTAGAGGTTTTATCTGGAGTTAAAGTTTCAAAATCAAAGTCATCTAACTTCATTGGATCTAATGGCTCTAACCCGTGGATGTTCATGCTGCTTAACTGATGATCAAACCCATCTAAATCAAGGTTTAACCCACTGTCTAGGTTTTGGTAGACAAACTCATCCAATTGTTCATTGGACATCTTGTCAAAGCTACTGGAGAAGTCCAAGAGATCTTCAATAATGGCTTGCGGTTCAGTCTCAAAGCTAGACAATGTTTCCATGCTGTTTAACAATAAAGGATCCTTTTCCTTCTTGTTAAAGCGCAACACACTGTCTAGCTTCGCCATCCCGTTTAAAACAGTATCACTGCAGAGCTTATAAACGGCTTTAAGTTCTTTTAGCGTAATCTGGATGTTTTTAATCTTGCTTTCGTTTTCCAAGATCGCTTTTTGAATGTTGCGCACTAACTCACCGGACTTTTGCAATTGTTCGTAACTGTCACTTGGACGGTGAGCAATCGTAAAGTCGTTGGATAAAGAGCGTAGCAGTTCATCCTTTTGGTACACCGAAAGGTCTTTAGCGTTTAAAAACTCCGCTTTGAGCTGTTCGTTTTCTTCAATTAAGCGGGTCAATTCGGCTTGTAATTCCTTTTTGATTAACAAGAAGCGGTTGGTAATGTCGGTTTTGGAATTAGTAATAAAGTCACTAATTTGGACAAACGACTTTTTGACCACCATTTGGTAACTTGACATTTTAAAGATGTCATCGAAGTTAATGGCAGGGTTAGAACGACCTTGCTTTAACTCGGCAATAATGCTGTTAATGTCGCTGTTGGCAAACAGATTAATTTCAGTAGTTGGCTGTAACTCCGTTGTTCTGAGCTGTTCTACCATCACTGGCATGAGTGGTTTGACTGGTTGTTGTACCACATCAACATTTGATGAAGCCTCTTTGGTTTCCACTGGTGGAGTGTCGACAAAGATTTCGATCGCAGGTGCACTTTCGACAACTGGGGTAGTTGGTGTTGAAATTGTTGGCTCTACTACCCGGTTGACCGTTTGTATGTTAATGCTAGATACAGCAGGTGGAACAGGAACTGTTTTCGGTTCGCTTACTCGTCATACAACCTCACTGTTACTGTGACAGTGGGTCTTGCTTTCCATACCCACTTCTGGTGGGGTAAAATCATCAAAGCTGTGGGTACCGACAGCACCGTGTTGATTGTGCTTAAAGTCCACAACCTCAACTAATGGTAAGTCAGATGAAGTTGTATTATTGAGTTCAATTTCAAACAGTGGTGGGGCAATTGTTGGTTCAACTGGACTGGTTGTAACGGTAGGAGTTGATTCCGCCACTTCAATTTTAATTGGGGTAGTTGGTTGTACTGGTGCAGTCTGTGGTTGGTTTTTTACTGTATCGGTGAGATGTAAGACATAATCCATATCAGCAACGGGTGCTACTGCTGGTTGAGGTTCTGCGGTAGCGGTAGCTTGGCTTTCTACTACTGTTACTGGAATTACTTCTGGTTGTTCAACAGGAGCAGCTTCTGGAGTTAGATTCCATTCATAAACCGCTGGTTGAGGTGCAGCTTGTGGTTGTGGTTCAACTACTTGGGTTGGTTCAGCCACAGCTTCTTGAACAGTTTCAACAACTGGTTCTGGTTGAACTTCTACAGGGGTTTCAGGTTGTGGTTCAACTACTTGTTCCACTTCTACAACAGGATTTTCAGGTTGAACTTCTAAAACTGAAGCTACTTCCGGGGTTGTAATTACTTCTGGTTGGACTTCCACAGGTTGTTCTGGTTGTGGTTCAACAATCGGTTCTACAGCTACTTCTGGTTGAACCATTTCAGATTGAACTTCTGCAACTGGAGTCGATTCCACAACCACTTCTGGTTGAACTTGTTCCGTTGGAATTAATTCAGACACACCTGCCACAGCTGGTTCTACTGGAAGTGGCTCGGCAACAGCTTCCGGTTGATGTTCTACAACAGCTACTACAGGCTCTGTAACTACCTGAGGTGTAATCTCTGCAATAGTTTCAACAACTGGTGTTGGTTCAGTTTGTTGTGCTTCTGTAACTGGCTCTGCAGCTGCTTCTGGTTGAACCATTTCAGATTGAACTTCTGCAACTGGTTCTGGTTGAAGCTCTACTGCTGATTGTTCAGCTGGAATCTCTGGTTGGGTTTGTACAGCTTGTGGTTCAACTTGTTCCAATATTGGTGCAGTTTCTCCAATTGGATCTAGACTTACTTCTAATTGTGGCTCTACAGCTACTTCTGGTTGAACCATTTCAGCTTGAACTTCCACAGGTTGTTCTAGTTGTGATTCAACAATTGGTTCTACAACTACTTCTGGTTGAATTTCTACTGCTTGTGGTTCTACTGTTGCAGTAGTTTCTCCCACCGGTTCTACAGCTACTTCTGGTTGAACATCGTAAACAGGCTCAGCAGCTACCTCTGGTTGTACCTCATAGCTAGGTTCAGCAACTTCTGGTGCTGGTACATCTGCTTCCAAACCAAAGTCTTCTGAAGCTGTAGGTGTGGTTTCAATGTTATCCTCAAAGCCAAACTGTTTCTCTTCCGCTTCGGTTTGGGGTAAAGTCGAGATCCATTGATCACCCTCAAAGTAACCAGATCAAACCCACTCCCCAGCTTCATCATAGTAACCATAAGCCTCATTACCGACATACTGAAGGTAATCCGGTTCTGCTTCGGTTTGTTCCTGTTCCCCCGCTAAAACTTCCACTGTTGGTGCATCAGCAAACACGAACGAATTACCATTGGCGTCAAACAACACGCTAATGTCGTATTCCGTGTCACCATAAGGATCATAGTAAACTCCAGTTTCACTGTTAAAGGCAATGTAATAACCATTGCCATCACCGTCATAGGCAATCTTGACTTTGCCATCTTGAACAGTTAATTTACCATCGTACAAATTACCAAAGATCTGTTCGGGATTTTCCTCAGTGTAATCCTTGTCTTCAAAAACTGCTTCTTTGCTTTTCTTCATGAAAAAAGAGCTTACAAAAGTGTAAGCATGGTTAACTTTGATTATAAAACTTTCTTTAAGGCCTTATCTTTTCCATGAGAGGTTGTATACCCTTAAATAATTTCTTAAATTATTTTCAGCAATCCTAATTAAGGGCTTAAACAAGGCCAAGTACAAGGGCATTAACAACGCTTGTACCAAGACCCTTGGCATTAAAAAGAAATAGCTACCATACTTAACGAAGTTCTTACTTGGTTTACCACTTAAAAAGACGAGGTATTCAATCGAGGCTACAGTGCCAATTAACAATGATACCACTAAGGTCATTAGTAAGGCCATCATTAAGATGTAGAGGTTTAAGATCATCTCTTTCGGTTTTTTGACCTTAGTGTACTTTCAACCCAAAAAGATTTCTAACCCCACAAAAAAGCTAATAAAGGCTGCCATCGTAATGCCTTGTAGGATGTGGACATTGACATCAAAGCCCCGGCCAAAGCTCTGGTTACCAGCAGCTTGAAAATTGCCATCAGTCAGGTACATTAATAATAAGACTAGGGTCAAGACAAAGAAACCAATCAGAATGCTTTGTAAGATCCAAAAGTCAATTTTTCAGTTAGTACTACTTTGGCGTACCTGATAAACGCCCTTAACCAGCCCCGCTAAAAAGCAGAACATGGGTTTTTGAATCGCAAACAACCAAAACCAGGTATAGCCCCTAGTAAGCCAGTCCATCGTGTCGGCAATGGCGCCAAAGGTAAAGCCCCACACCGGTCCAAACATCCAACCAATTAAGGCAAAGGGAATTCTTGTGAAACTCACCTTGAGCACACTAGTGACATTAATGGAAAAAATATTAAAGATAAAGCTTAGTGCCATTAACACCGCCGCTCATACTAACAATTGTAAGTTCCGCGTTACCTTTAGGGGGTAATACGGAAACAAAAAGATAAAGAAGGCGTGGTACTTAGTTTTTAATAGTTTGAAGGTCTAGATTACCCAAAAACAGCTGGGGGTGTTAAATCATTGTCGTTGTTAGGATCACAATCCGTTTTAAATGGCTGTTGCATTTCCTCTTGCACGTAACGGTTCAACACTTCGTTTGTTTGATCCGAACTTGGTTTAGGACTCAAAACAAAGGCAATAATACTAATGAGGGCAAAGGGAATAATGCTCGCTAGGTAAAATATGGAAAAACCAACAAAGATAGTTTTAAAGGTCTCACTGTTAATTTTTAATTCATTAGACCTTAAGGCATTTAACAGAAAAGAGGGGTTTAAAATACCACCGGTAATAATGTTACCCCCAGGTAGGGCATTGAGTGCCACAAACAAGAAGATTGTCCAGAAGATCCACTTAATTCTGACCTTATACCGGTAGTTGTCATTGATCCTGGGAAAATCAGTTACCCCAAACTGGTGCTTGAGAAAGTGCATTGCCTTAAAGGTTTGACTTCTAGTAGCAAAACTGTAGGGCTTACCTTGAAAGGCTAAAAGGAAGGAAGTACTACCAAAACCCAAGAAGATCAAGATAATTAAAGTAGAAGCACTAACAGGGAAAAAGAGGTTAATGCCACCGTTTAAATCAACTAACGCTGGCCTGTCGGTATTATTCTGACCAGTAACCGCTTTAGTTAGGTTACTGTCAGGGTTTACATACCTGTAAAACAAGAGGTTTACCACCACGAGGTAAGCAATGGCGGCCGCTCCAATTAACAACATGGAGATAAAGATTGCTAACTGGGCTCACTTAATTCACTGGCTTTTAAACTGGAATTCAAAGATTTTGCCTACCATCCGTTCCGAGGTGACCCGCATAAAGGCACGTTGGCGTGCACGTGCTTCGAGTGATTCGTTGACAAAGTTTTCCAACTCTGGGCTTACAGTACCAGTTGTATGTATCCGTGGTTGGTTTAAATCAATTGTAACCGTCGGTTTATTAGCTCCATTGGTTGTTTTTTCAACAGCTGTTGGTTGAACGCTACTTAACTTACTAAAGCTATTTAAATCACGGTTAAAGAGATTGCACACGGCAATTGGTAAATAGTTAGCCAAGTCCGTGGCATCAGTTAAATCAAGTTTGAGGTTAAGTTCGGTGTTCGCTAGTTTGGTTTGCCCTAAACTAACAAAGCGCGCTTCTAAGTTTTGAAAAACAGGTTGAAAGTCCTTTTTCTTGGTTTTAGCCTTAGCTTGCTTTTGCTCTAAAAAGTACAACAGCTTTAACTGTTCGCGAATTGTTTTAGCAAACGCCGGGTCGCTTTGGATCCGCTCTTTGAGGGCATTGGCATTAAAAACAATGTCTAAATTGTCTTGTAACTTGGTTAAAAGATCGCTAAAAGTCATCTTGGTATAGTTTACTTTTGCGCAAAGAATCGCAACACGAAGTGCTTTAGCAAATGGATTGCTAAGCCATCATTGCGCTTAATTTTAATATCCAGCTGCCAAATCGCATTAATTAAGGCCGTTAATTGGTTGGTGGATCAAAAGCGGTACTGGCTTTGAATCGCCTTTAAACGAAAGGGATTGACATTTCACTGTAGTTGGAAAACAGCTTGTGGTTTAACGCCTTTGACCATCAAGGCCAAAAGTAGTTCATTTGCTAAAAATTCTAGGAATTGCGGTGGTGTGAGCTGCTTTGGTAAAAACAAGCGTTCAATCAACTTTAAGGCTTTAGCTGTTTGCAAACGCACCATGGCGTCAGTTAATTGTAAGATTTGACTGGTTTGGTAATCACACAAAACGGCTTCTAACATTTTTTTGTCCTGTAATTCTGCCGGGCTAAGTAGAGTTAACTTACGCAACTCCTGTTCAATGACCCCTAAGTTAAAGGGTAGAATCGTGGCTAAGTGGTCAATTAAATCAGGGTTTAAGTTGAGTTTGAGTGTCTTAGCGGCCTTACTGATCACCTGGTGGATGGTTTTAGCTGTGACTGGTTTACAAGCATACTTGGTAATTGCCCGGTCATTAATGAGGGCATTAGCGGGACTGTCGCTGTAAATGGTGACCACCACATCAGTATCTTTAAGTGTTTTTAAGCAGTGTAATTCAGCCTTAGTTAAGTTGTTCTTTTCCAAAAAACTGCAGTTGTGAATGAGGATCGGTTTAGTGTCCTCAGCAAACAGATTCTGGTTTAACACCACATCATACAGCTTATTAACATCTTGAAACCAGACCTGTTTACAAGCGGCTGGGTTGAGCAGTTGGTTTAACTGCTGGTGAATTAACCCAATATCGGCACCATAAACTACGGTCATAGTAAAAGACTGTACCCACAGTATACAACAAGCCCCACAGCCCATAATAAACCAGGTAAAACACCCAGTTAGGTTTGGGTAGATTTAAAAAGACGGTCGTGTTTAAGGCTCACTGACTCAACCAGGGAAGTGGTCAGTAAAAACCGACCAAACTGTTGCCCATTCATGGAATTAGCGGTAACAACAGTCAGCTAGCACAGAAGTAAAACAAGGCTATGGGAGTAAACAAGAGGTTATGAAACACACTTAAAGCGTTTAATTTTCGATTTAAGTGCAAAGTAATCGGACTGACCACAATGAGGATTAAGGTGGAAGTTAACACCGCTTGCAGTGGCTTTCACAGCTTCACCTTATTAATGGCAAACAACACAAAGCACGCTAAAAAGGAAAAGTTAAAGCCAAAGTTGTGTAACGCTCCCGGACTCATTAAGATGATTAACAGCGCCGTTAAGCCCAAATTATTTTCGGGTAGTTGCTTGGGAAACATTTGCCGTAACAGCTTTGAGACAAAAACCCGTAAAGCTGAAAAGGCAAATCCGACCAAAAAGAGGTACAGCAACAGCATTACAAAACCCGTTATTTTATTGATGTAAAAGCGCTTGTACAACCAGCGCTCCATGACACCAAACAAAAAACTAAGGTGAAAACCACTAATCACAAACAGGTGGACAATATTGAGCTGAATGGCACTCTGATACAGTGTGTTTTTTGTTGTTTCGTTAATTAAAAACAGCTTTAAGTACTGATTAAAAACACCACTAGTGTGTTGATCTAGTCAACTAATAAAAGGGGTGCGAAGTCAGTTCGCTTTCATTAGTTTGGCTAGTTGAAACTCTGGCATTAACTGACAGTATGCTAGTAAAGCGGCACTACAACTGATTAACAGTAAGCCAAACTTCCAGTGTACCGTTAAACTAATTAGGTTAACTATCACCAAAAAACTCGCTAAAATTACCCACTGATTTTGTTCGATAAAGAAGTAAGCAGGGGTAAAGGTGAGTAACGTAAAAAAATAAAACAGCTTTCACTTCATGCTGTTTTATTAATTGGTTAATTAGTTTAGAAATGCCAATTAAAACTCTTTACTCCAGCTGGAACGGAGCGGAAAGCGTTGAGACAAACCACCACGTAACGGTGAGCGGTAACGCGGGGTGAAACTGGTGTAATTGCTCCCTAGATCAAAGCGGTGCGTGTTGAAAAAGGAACTGCGGTTACGCCTTAAGCTCGACAATTCCAATGGTCTTCTAAGTGGGTAAAAGCTACTTGGACTGCGGTAAGCGCCAAAGCTGTTAAGGCTGTCGAAGTTCAAACTCGCTGGGTTGCGGTAGTTTACAGCCATCGGTGTAACACCAGTAAACCGACTGGCAATTGGTGTTGACTCTACTGTTGGCCGTGTAAAACGTGCTCAACTGTTGCGGATTGCTGGAAGGTTAAACAGCGGTTCGTTGTACTTGGTAGCCACATATTCACTCGGTGGAGCTAACTGGTAGCGCTCGTGCGGTAAAGGCGTACGGGTAGAAGCATCGTGGAGGTGACCCAAACTCGCTAGCTTTTCCAACAATAAGGAACGGTTGGTGGTGTGAATGGTAATTGCACCACTTTCATTATTAACACAAGCTGACTGACACGGTTGTGGTTCCACTACCGATGCAGGGGATGGTTTTCATTCCGGTTCGGGTTGTGTTGGACGGATAATTGGTTGGACTACCACTTCTGGTTGAACTACAACTGGTGGAACCGCAGGAGTTGTTTGGACTACCTTAGGTGTCACTTTAGGTGTAGCTACTTGAGGAGTAACTACTACCTGTGGTGCTGGCGTTGGTGTTACTACCTTCGGTTCTGCCTTAACAACTGGTTGAACTACAGCAGTTGGTTGGACAACTACTGGAGCAGGGGTTGGTTGTGGTGCTACTTTAGGTACAGCTGGAACAGGGGTTGGTTGGGCCACTTGGAGTGCAACTTTTGCTGTTACTACCTTCGGTATTTCAACTTTAGGTGAGTCAACCTCAACAGTTACAGGTGTTGGTTGTGGTTGTTCCACTACCTTAGGCTCTACTTTAGGGGCAGGAGTAGCTTCTACCACTGGTGTTGGGGTAGGTTCCACTACCTTAGGAGCTTCCACTACTGGTGCGGTCTCTACCACTGGTGTTGGGGTAGGTTCTACTGTTGGAGTAACCTCTACTATTGGAGCAGATTCAACAATAGGAACAGCAGTTGGCTCCACTACTGGTGCAACTGGTTCTGGTTGGAGTGGTGCTACCTCTATTGGTGCTACAGCACTAGCTTGATCAGGATCGCTGTAAGCTTGGGAATCATAGTAAGCGTTCGGATCGTGAGCTTGATCATAGTTCTGTGTATTGTAAGCATTAGCATCATAGGCTTGATCATACGCTTGTTGATCAAAGGACTGATCGTAAGCTTGCTGGTCATAGTAAGCGTTCGGATCATAGTTTTGGTCATACGCTTGCTGGTCGTATGTTGCTTGATCAAAGGTCTGATCATAAGCTGGTGGTGCTGTAGTATCCTGATATTCGTCTGGTGCTTGAGGTAGTGGATCGAGTCCGAACAGTTGATCAGCTTGCTGTTCGGGTGTAGCATCGGGATCCACAACTGGTAAACCAGCATCAATGTCCGGATAGCCCTCACCACTCAATGGTTCGCTTGTAGTGGTATCTGCACTTGCGGTACTACCACCATAGTTTAAATAGTCCACTAGGGGAATGTTTTCCCCATCACGGTAACCACTCACATCCAAAACATTGCCATCTTGGTCGTAAAAGGTATTGTTGACCTGGTCGTATTGGACTATTACCTCATTGTTGTTGCCATCAAAGGCAGTGAGTGGAATAATCTTGGGGGCAAAGCTAATTGGTTCATCACTGTCCAATTCGAACAGAGGATAATCCGCTGGGAGCTGTTTTCTTTTTTCCAAAGAAAAGATGTTCCCGGTTTCAGGATCCTGGTACAGGGCGTGAAGCTTATTTTTAGCATCACGACCATAAACCACATTAATGTCGGGATAGCTCTTTTGGATCTTTTGTGCTAGCTTACCATACGCTTTCGCTAGCTTTGCTCTTTCTTTATCTGTCATCTTGAAGCACTAATTAGCGTTTTGGTGGAAAACCGGGTTGGGGTGGCATGCCAGGACGTGGTGGTTGCATTCCGGGACGAGGCGCCATACCGGGTTGAGGTGGGAAACCAGGTCTTGGAGCCATACCGGGGTGTGGCGGCATGCCTGGACGAGGCGCCATACCGGGTTGAGGTGGGAAACCAGGTCTTGGAGCCATACCGGGGTGTGGCGGCATACCTGGACGAGGCGCCATACCGGGTTGAGGTGGGAAACCAGGTCTTGGAGCCATACCGGGGTGTGGCGGCATACCTGGACGAGGCGCCATACCGGGTTGAGGTGGGAAACCAGTACGGGGACCGAAGTTAATTTGGACTTGGGGTTGAGGTGGTACTGGTACTGGTTGTGGTGCTGGTTCAACTTCCGCTTCAGCATGGGCTTCAGCAGCTGCTTGTTGTTCTAACGCTTGTTGCTCTTCTTGTTGGGCAGAAATGCGTTGTAGCTGTTCCGCTAACTGTTCTTGGCGTTCCTTTTCCTCCAATAAGCGTTTTTCCTTGCGCTTCACAATTGGCAGTCCTATAGCAAGGCCCAAGATGATGGCTAGGGCACTAAAACCAAAGCAACCAGCAACTGTAGGAATAAACCATGCTTGCTCAGTAATCGGCTTGCCCTGAATTTGTACTGTATCCTCTTCTGCGTGTAAAACAACGTTAAGGGGACTCAATTCACTCTTAACTTCTGTCAGTTCGGTATTGTTGTGCTGTACCAAAATTAAGGTTGCAAGCACTATTAAAGCGCTGAACAGCACTAGCATTCAGGCTAATAAAAACAGTTTAAGCTTTCTTCGAGGTGGTAACTTCATGCACTAATTAAAGGGAGACACTAACGGCGGCGGCGACGGGGTGCTTCATCATAGTAGTAATCATCAACACGACTTTGTTGTGCCCGTTTTGCTTTACGGCGCTTTTTGATGATGGCACGGGTAATCTTGGACAGGATAAAGTTAATAATTAAGGCACCTATAAGCCCTGAAACTAAGGCCATGCCCAACGGAATAATGTACTTAGTAAGGTAGGGACCAACCTTTTCTAAAAAGGCGGGGATATTGGGTTTCTTTAAAAAGTCGACATACCCTGGGATTTGGCTAAACAAACTACCATCAATCGGAAAGCGGGGATCTAGCTTGTTAGCATTATTAGCTACTTGTTCTGCTGCTAGTATCAAGGCGTGGGACTGTGATGCTGCAGCTGCTTTAGGTGCAGTTACCGTAGCGCCTACACCACTAGTAGTAACCGTGTCAAGCGCTTCCTTAGAGAAAAAACGGTTAAACACATCCATTGGTTGTGCTAGTTTAAAGGATGGGCTAACAGTACCAAAGTAAAAGATTGCTAAAAAGAGGTAAGCAATTGCTACCGTCAGTACCGCTAAGAAGGCTAGCTTAAAGAGCAATTTGAAGAAACCCATTGCTAGAGAAGAGTTAAATATTGGGAAAATTTTAACTTTTCAAAGCTTTAATTCCCCACTTACAGCTTAACTTCCTTAATTAAGAGCTTTAAAAAGCTCTGCTTAAATTACGCGTTAACAAAATAATGCGCAGGATAAAGTAGACCAAGTTCATGAAGCTACTTACCAACCAAGCGCCAAAAAAGAGCGCACTACGCCACATTTGTGCTTTGGCTAAAGCTGGGTCTTGACTTTCATACAGTTTCACCAAGTCCTGTTCGTTGCGAATTTGTAGACAAACAAAGTAAACCGATAACAAGGAAAAGATCCCACCAACTATTAACGAAAGTCCGGGGAACAGTAAATCCAAGATATTAGTGCGACCACCGCGGAACACCGTAAAGTTCAACACAAAAGTCAATAAGCTCGCAATTATGCTAATAAAGAAGGCTCAAATAAAGATACGAAACAGCGTTTGGGTTTGTTGGTAACGGCGTTGCTGGCTCGGTAATGCACTCAAGCCCCAGACCGTCCCATACACTAATGCTGCCCCTAAAAAGGCAAAGACAATGTCACTAAACTTAAAGTCAATCAATTCACGTAATTGTGTGGTGGCTAATGTTAACAATACTCCTAATGTAATCCCATAACTAATCACATCGGCAATAATTAAGCCCCAGAACCACGCTAAGGTCGTGTTACGGTTCCTTAGTTTAAAAGCCGTGACAAAGTAAAGGATTAAGGAAACCAAACTAGCAACAAAAGCAATCGTACCCAACGTTCTTATATCACTAGCCAGTGTGCCAAGGCTAGTAGTGTTGGTTAACAGCTGTTGTACCCCAAAACCAATTAAGAAGATAAACAGAATGCCAATCGCGGCAACAGAAAAGGTCAAGCGAATGACCGAGAACCGCTTGTGATGGACAAACTGTTGCTCGGAAAAATTGACCTGATTATTAGCTTGATTGATCATGATAAGTCAACTGGGTCAATTTTAATTTGTAAGCGCTTAATTGCTAATAAATGCAAAATTATAGAAAGGGGATACTTACCCAAGTGGCTGAAGGGGTAGGCTTGGAAAGCTTATAGATGGGTAACACCATGCGAGGGTTCGAATCCCTCAGTATCCGCCACGGAGACTTACCCAAGTGGTTTAAGGGGGCGCTCTCGAAAAGCGTTAGGTGTTTTTGCATGCGTGGGTTCGAATCCCACAGTCTCCGCCAAAATATTAACTGTATCAATTAAAAAAGCACACCTTTTGGGGATGTGCTTTTTTTAGTGTTTCCTAATACTGACAACTATTTATTTTCGGGAATAAAACCATAGTCACGGATGGTACCGTTACCCGATGGCGCACTGCGGAATTGGAACCATGGTTTGAGCAGCGTTGGTGATGTTTCGTTCAACCCAGCTGCTGGTACGGGTAAAACCGGGAAGTAAGAGTCGACTACGGTAATTAAGAACTTAGCGTTATCGGTGTCTTTTTCCGCTCAAATACTGTTAAAGCGGTTGAGCTGGGCGGCTTTCTTATAGATATCTTTCAACTCGTAACCAATGAAATCTTCGTTTTTACTTGGTTTATTTGTACCGTTCTCTATCTTGTCACGCTTTCCAAGGTACTTATTTGGATCTTTGATAGCTTCCTTTACTTTTTCTTTGTCTAGAATGCCATTATCCACTAAGAATTTGAGCAAGCTTAAAGCCTTTTTGGTGAACTCTAAAGATGCTTGAGTGCCGTTACCACTAGTTGTAGAACCGGTGCTTCTTTGTGCAGCTTGTGCTTTAGCATCACCAAAGTTCTTTAGTACGTAAGGGTCATCTTTGAAGAGGTCAGCGGAAGTTACGTCATCCCACTTTTCTTCTTCAGTATTACTCTTGCTGCTATCTTGAGATGAATCTGCTTTCTTAACAGTCACATCAGATGTGGTTGAAGCTGTATTTTGTTGATCTATTTTTTGTGTGAATTTACTATGTACATCTTTTTGACTCGCAGCTAACAACAACGTACGTACAATGTGAGCGCCCGAGGAGTAGGTAATAACATCACCGCCACCTTGATCGTTCAATTGGGTGGCTGCGTCCAATCAGGTACCCACACCATCATAGTCAGGGCTTCAGGAACTAAAACCTAACACCGAGGACAACCCAATGTACCACTCATTTCGAGTTGGTCCTTGGGCATCACCTAAGATAATTTCTGGTACTATTAGTTTTTCTCCGTTGTCAGCAGTACCCAATTCAGTGAGGGCTTGCTTCACGGCGTTGTAGTAATCCACCCCGTTAGCGGACAGCGAAGAGTTCCCTAACTTCATGTTGAAGTGGAGGGGATTGCTACCAGAGAAACCAACCTTCTTTAAAGCTTGAACAAAGCTGTGCTTTGTTACGTTTACACTCTTAGCTTCTACTGAAGAGGTTTGGTTTTGTTGTGTGTTGTCTGATGTGTTGTTGTTCTTAGCAAACGCTGCAGAGGCAGCTGCTCCAGCCTGACCACTTGTTGCTTTACTGCTATAATAATTAATCTTAACAGTCGCACTCTCCAAAACGTTTTTATTCTTTTCGTAGTTACCTTCACGCTGTGGAATCAAGCCCAGGCGCTGTTCCTTCTCGATCTTATAGTAGTAATCGTTCATCAGATCTGAACCTTCGGTAGTTGAGACAGCTTTTTGTTGAACACTTGCACCATTTTTACCCTTTTCCTTAAAGATGCCAAAAGGTACGATCGAATAGTTCAGGTCACCGGAGTTTGGCAACAGGATAATCGCTAGGTTTTGTCAGTTAATTAAACCGTTAATGCCAGCGCGAATCGTTAACGCTTCTTCTGACTCACTGTTGTACAAGAATTTAGCCTCGGTTTCGGTAATGTTGGAGTTAGTTTTAACGGTTTCACCTTCCACTACGTAAGGTCGTGATGAATAGGTAATCTGGGCACTTTGCGCGGTCTTTTGCACTTTAACCCAGCGTAAGTCCCCCGTACCCGCAAATCGTGACCTAGCATCCTCTTGTTGGGAGTATGGCACCTCCGCAGAAGATAATCCTCCAGCGATATAGTTGGAGTAAAACACTTCAGGGGTAGCGCCTGGTTGGAAGTAGTTAATCATCGTTGGGATGGGTTTTTCGTTGTCTTCCCGCGTTTTTGGTAAGTTGGGGGTTATATGAGTATCATACTCACTGTTACGCTTAAAGACAATCTGGCTCTGGTTAAAACTCTCGACATAGTAAGGCCCCACTGATCAAGTATCCCGTCATGCGTTAACCCCACCACCATAGATACCATCAAAGTTAGTTTTGGTTTGGTCTAAGATTTTCGACCCATTCTTTTCGTTGTAGACCAATGGTGAGTCCTTACCTACCTTAATTGCTTTTACCTTCGGGTGGGTATGAGGAATAGGAAAGAAGAATTCCTTAGACATCATACTCAACAAGAAGGGGAAGGGCGAGGTGAGGTAGACGTTGAATTTGCTGTCATCAGTGCTTTGATAATCCTCAGGATTGTAAGCATCATCCGTTATCTTAACAGTTTTTTCTTCACCATCTTTTTCATCAGATGAAGCAACCTTCATTTGAACTTGGGCACCATTCTTCTTGGTCATCCCCACGGTCTTTTTCACATCCAAACCCATTAAGTCAATGAAGTAACCGTTGCGGTTAAACCTGAGTTCCGATGATAAGATGTAAGCCTCAAAACCGCGTTCAAAGTCTTTGGAAGAGAGTTTGACATCATTGCCACTAGCGTCCTTTACCGGTTTACCGTTATTGTCGACCCATTTGATACCAGTATCAATGGCGAATTCGTAACGGGTGGCCTTTTGCGAAATTTCTTGGAATTTTTCTTCCTCAGTAATCGACTTCGATTGTTGATTATTTTGACCGTTACTTGAACTTTGTCTTTTAATTTCACCAATGCATTCTCAATCATCTTCACCACTAGTGCTAGTACCACTAGAAGTGGTAGTGCAAGAAGAATTAGTTGAAGTTTTTTGACTTGAAGTAGCTTTATCCTTCTTCTTATACCCCTTCACTGAGGTAGCCAATTCCAACACCAATTTCTCTTGGGTTTGAGTGGCTACATTGACACCATCATCTTTGTTGGAACCAGTGGCAATGTAAGTGGTTAGGCCTGCATACTTGCTGCCAAAAAAGGAAGAGGTCGGGGACACGTTGAACATACTCATCGGTACCGAATTGTTCTTTAACAAGTCGGATGCGGATGAGGTGACATACACATCGCGGGCTTGGGCAGCGCAGGCACTGAGTGCTACTGCTGAACTTACCCCTAAGGTTGATAACAAACCCCAGTATTTTCGCTGAAATTTCATGGATCGTTCATAAATGTTGTTGATGATGTTTATTGCTATATGAAAAACATGGCATGAAAGCCATGTTGAACCATGAGCAACATACTCTTAGCTGTGAGCTACTAAAAGTGATTTTATTCTAATTTGGGAGCTTGCTAATTTGCGCAATTAAAACTCAGGTTTTAAAAATTGCTGTTGCAATAAATTAATTCTTAATTTAAACGCTTCAATATCTGCCACTAATACTTACCCAAATGGTTTAAGGGGGCGCTTTCGAAAAGCGTTAGGTTTTTTTGCATGCGTGGGTTCGAATCCCACATTCTTGGCCAAAATATTAACTGTATCAATCAAAAAAGCACACCTTTTGGGATGTGCTTTTTTTAGTGTTTCCTACTACTAACAACTATTTATTTTCTGGGATAAAACCATAGTCACGGATGGTACCGTTACCCGATGGCGCACTGCGGAATTGGAACCATGGCTTGAGCAGCGTTGGTGAAGTTTCATTTAATCCAGCTGCTGGTACCGGTAATACCGGGAAGTAAGAGTCAACTACGGTAATTAAGAACTTAGCGTTATCCGTGTCTTTTTCCGCTCAAATGCTGTTAAAGCGGTTGAGCTTCGCGGCGTTTTCGTATAAGTCTTTGAACTCACTACCAATAAAGTCATCTTTAGTTGGCTTTTGATTGTTGCTGTCTAACTTACTACGTTTACTTAAGTACTGATCCGGATTCATAATAGCTTCCTTCACCTTATCCTTCTTCAAGATTTTGTTATCTACCAAGAACTTAAGTAAGCTTAGCGCTTTCTTGGTGAAGGCTAGGGAAGCTTGGGAACCATTACCATTTGAATTAGTGCTTCCTCCACTTTGCATCTTAGCTTGAGTGGTACTATCACCAAAGTTTTTCAACACGTAAGGGTCATCTTTGAAAAGATCTGCCTTAGAAATATCTTTTCAGTCTTCTTCCTTTTGTTCACCTGTTTGCTGTTGTCCATCACCAGATTTTGCAACTGTTACTGCAGTAGTACTGCTAGTCATAGAACTAGTCTGATCATTACCCAACGCTTTGCTTGTAAACGTTGAATGTACATTACTCTGACTCGCAGCTAGCAACAGCGTACGCACAATGTGAGCTCCTGAAGAATAGGTAATAACATCACCACCACCTTGTTCGTTCAACTGAGTCGCTGCGTCTAATCAAGTGCCCACACCGTCATAGTCAGGGCTTCAGAAACTAAAGCCTAACACCGAGGACAACCCAATGTACCACTCGTTCCGGGTTGGTCCTTGAGCATCACCCAGAATAATTTCTGGCACTATGAGCTTTTCACCGTTATCAGCAAGTACCCAATTCGGTGAGTGCTTGCTTCACGGCGTTGTAGTAATCCACACCGTTAGCGGACAGCGAAGAGTTCCCTAACTTCATGTTGAAGTGGAGGGGGTTATTGCCAGTAAAGCCGACTTTTCTAAGTGCTTGAACAAAGCTTTGCTTTGTTACTTCAACTTTTTTCGCTTCTACCGAGGGGGTTTGCGATGAAGTTGTGGTTGAATCACCAGCAGCTTTGTTTGCTTTAACAGCCACTGCCGCTTTAGATGCGCTTTGCGTTTGACCCTGTTTCGAACTATAATGGTTAACCTTAACCGTTGCACTCGTTAAAACGTTCTTGTTCTTTTCGTATTGACCTTCACGTTGTGGAATCAAACCTAAACGTTGTTCCTTTTCAAACTTAAATCTTATAGTAGTAATCGTTCATCAGATCTGAACCTTGGGATGCTTGTCCAGTGTTTTTTTGTTTAACTTGAACATCCTTACCCTTTTCCTTAAAGATGCCAAAAGGTACGATCGAATAGTTCAGGTCACCGGAGTTTGGTAACAGGATAATCGCTAGGTTTTGTCAGTTAATTAAACCGTTAATGCCAGCACGAATCGTTAACGCTTCTTCTGACTCACTGTTGTACAAGAATTTAGCCTCGGTTTCGGTAATGTTGGAGTTAGTTTTAACGGTTTCCCCTTCCAATATGTAAGGTCGGGATGAATAAGTAATCTGGGCACTTTGCGCGGTCTTTTGCACTTTAACCCAGCGCAAGTCCCCCGTACTCGCAAACCGTGAACGTGCATCCTCTTGTTGGGAGTACGATACCCCCGCAGAAGACAATCCTCCAGCGATATAGTTGGAATAAAACACTTCCGGAGTTGCCCCTGGTTGGAAGTAGTTAATCATCGTTGGGATTGGTTGTTCGTTGCCTTCCCGCGTCTTTGGTAAGTTTGGTGTGATGTGATCATCATACTCCTTATTACGCTTAAAGACAATCTGGCTCTGGTTAAAACTCTCGACATAGTAAGGTCCGACTGATCAAGCATCCCGTCATGCGTTGACACCACCACCACCATAAATCCCATCAAAATTAGTTTTGGTTTGGTCTAATATTTTGGCACTGTTCTTTTCGTTGTAAACCAATGGTGAGTTTGGACCTACCTTAATTGCTTTTACCTTCGGGTGGGTATGCGGAATAGGAAAGAAGAATTCCTTCGACATCATACTCAACAAGAAGGGGAAGGGTGAGGTGAGATAGACGTTGAATTTATCGTCACTGGTACTCCTGTAGTCTTCTGGGTCGTAATTGTCATCGGTGATTTCAATCTTCTTTTCTTCACTCTGTTGAGTACTACTGGAATCAGTTGTCATTTTTTTGACCTGAGTGCCATTTTTACCGTTGGTCATCCCCACGGTTTTTTCCACATCCAAACCCATTAAGTCAATGAAGTAACCGTTGCGGTTAAACCTAAGTTCCGATGATAAGATGTAAGCTTCAAAGCCACGTTCGAAGTCCTTGGAAGAGAGTTTGACTTCTTGACCTTTTTCGTCCTTTACCGGTTTACCATTGTTGTCAACCCATTTGATACTAGTATCAATGGTGAATTCATAGCGGGTGGCTTGTTGGGAAATAGTCTGAAAGTTTGAATCTTCAGTAATGGACTTGCCGTTGTCGCCTTTTCCAGATTCACGTTTAATTTCTGAACCAACAACTTCTCATTCATCCTCAGTACTTCCATTGTTTCCAGTAGAAGAAGAGCTCGAAGAAACAGAAGCAGTCTTCATGTTAGATGCGGTACTTGTTTTCTTCTTATACCCCTTAACTGAGGTAGCCAATTCCAACACCAATTTCTCTTGGGTTTGACTGGTCACATTAACGCCATCGTCCTTGTTGGAACCAGTGGCAATGTAAGTGGTTAGGCCTGCATACTTACTGCCAAAAAAGGAAGAGGTCGGGGACACGTTGAACATACTCATCGGTACCAAATTGTTCTTTAACAAATCGGATGCGGATGAAGTAACATAAACATCTCGTGCTTGGGCAGCGCAGGCACTGAGTGCTACTGCTGAACTTACCCCTAAGGTTGATAACAAACCCCAGTATTTTCGCTGAAATTTCATGGATCATTCATAAATGTTGTTGATATGATGTTTATTGCTATATGAAAAACATGGCATGAAAGCCATGTTGAACCATGAGCAACATACTCTTAGCTGTGAGCTACTAAAAGTGATTTTATTCTAATTTGGGAGCTTGCTAATCTGCGAAATTAAAGCTCAAGTTTCAAAAATTGCTGTTGCAATAAATTAATTCTTAATTTAAAGGCTTCAGCAAACGCTAAAACTTGGTGCACTCGAAGGGACTTGAACCCCCACTCCACAATGAAACTAGATCCTAAGTCTAGCGCGTCTACCATTCCGCCACGAGTGCATATTGGTGCATCTTGAGGGGCTCGAACCCCCGACCCAATGATTAAGAGTCATTTGCTCTACCAACTGAGCTAAAGATGCATTAAGGGAAGCTGGTGCCGACTATAGGATTCGAACCTACGACCTACTGATTACAAGTCAATTGCTCTACCAACTGAGCTAAGTCGGCCTGGTGGATTGTGAGGGGATCGAACCCCCGACCCTATGCTTGTAAGGCATATGCTCTCCCAGCTGAGCTAACAATCCAGGCTTTTGGTGCCGAATACAGGAATTGAACCTGTAGCCTATGCATTACGAGTGCATCGCTCTACCCTTGAGCTAATTCGGCTTGGTGACGCGTACGGGATTCAAACCCGTGAATGCACGCGTGAAAGGCGTGTGTGTTAAGTCTCTTCACCAACGCGCCAAAATTCTTGGCGGCCACAACAGGGATCGAACCTGTGACCAACCGGTTAACAGCCGGTTGCTCTACCGCTGAGCTATGTGGCCAATAATGCAAGTACAATTCTAGAATTTCTAGCCCACTTTAACATCTTCCCGCGGATAAGAAAAGCTGTTTCCTTGAGGGTGTTTTTTAACAAAAGTGAGTACCGAACTGCTCACCCCGACCTGCCCCATAATCATTAGTAAACCTAACGTTAAGTAGTTAAACAGGTTCCGTGTTGGCTCTAGTGCCATGATCTTCGTGGCCCCACTAGACAGTCCGACAGTTCCAAAAGCAGAAGTGGTTTCAAACAGGCTGTCGACAAAGCTAAGCGGTTGTTGCATACTTAACGGCAACAGAATGGCAATAAGAACCACGGACACTAAGCCAAAGACGGCTACCAAGAAGGCATTAATCACCGTTTGCCCGTCAATACTACGCTTAAAGACCTTGACCTCTTTTTGACCGCGGAACTTCGCTTTTAACGTCAAAAAGATGACCGCTAAAGTAGTAGTGCGAATCCCTCCGGCGGTAGAAGACGGACTGCCCCCGATAAACATAGCAATCACCATAATCCCCTTAATGATGTCAGACTCAGCACTTCAGGGAAACACACTAAAGCCAGCGGACCGGGACGAAATCACCCCAAACCACAGTTGCATCACCCGACTAGCCGCTGGTATGGGACCAAAGATTAAGCTTTGGAGTTCTTGATTGGATTTATTTTTCAGTCCCTCCACTAAGTAGGCCTTCTCGACATTGAGATGGGCAATCGTATTGGTCAAACTAGTTATAACAATAAACTCCACCATTAACAGCATTAAAAAACACCAAGCCGTGACAATCAGGTTAGTGAGTACCGCTAACTTCGTAAACAAACTAAACTGGTGTTTGTAATACTTACCATACAATCGCCGTTTCTTAATCGCTTCATAAGCATCAAATAACACCGGATAACCAATGCCACCAAAGATAATTTGGCTAATCGCTAACCACTGGATCACGATGCCCCAGTTAGTGCGATACGGGGCAAACGAATTAGCGCCCAACAGGTCAATGCCAGCATTATTAACCGCTGACAAGGAGTGGAAAAAACCATACTGAAAGGCTAAGTTAAGGTTATGAACTACGGGTAATCTTTTGGTGGAATCGACTATGAGGGCCTTCAATTGGTTGGACACCTTACCACTACTTTGAAAAACACTAGCAGACTCAAACGCGGGGATAAAATAAAACAAAATGGTGTACAGAAAGCCATACAGTAACTCCACCATAAAGAGGAATAACACCGACACCACAATCATTTCACTAGTGGTCCCTAACTTGGAACCACCCCGTTCTGATTGCAACATTAACTTCTCATAAAAGCTGTACTTGCCCTTCTTGTGTAACTTAAACAGTCTTCAAACAAGGAAAGCAATCACCACAAAACCAATGCCCCCTAACTGCAGTAAAATGGCCAACACCGTTTGCCCAAACACACTGTAGGTTTCACTCACTACCGTAGTAGAGAGCCCCGTATCACTAAAGGCACTGACCGCTAAAAACAAAGCATCCAAAAAGCCATAGGAAGCCGTTTGTTTACTGATGGTATGACCCTGTAAATCCACCTCATAGCTAATAACCTTTTGGTAGTCGGTTTTGAGCGCAAACGGGAGGAAAAGCAGTACAGCCCCCAACAGGATGCAATAGATGTAAAAGTGGAAAATGCGCTGGGAAATACTGTCACCCCACAGGATTAGTTTTAACCAAGCGAGTAGTTTAGCTTTCTGTTTATGAGCAGCTGTCATGTAAATGACAATATAAATATAATTTTACCAGCATATGAAACGTGCCGATTTTTGCATTATTGGCCTTGGTAGGTTTGGGATGCAAGTAGCACATTCTTTAAAAGAGAACAACTTTACCCTGGTTTTGATCGATAATGATCAACACAAAACCAATACTGCTGCCCAGGAATTTGACCATGTAGTGTGTTGTGATGGGAGTAACTTAACGGCGTTAGCTGAGCTCCAACTAGAGGAGTTTTCGGCAGTCATAGTTGGGGTAACTAACATAGAAGCGAGCATTATGATTTGTGCTAACCTTCGGGAATTGGGTCAAAAAAACATTATTGCCCGTGCTAAAAACGAAGTGCATAACCGGGTGTTACGTACTATGGGTATTAGGGAAGCTTTGATCCCGGAAAAGATTGTCGGCAAGAACCTTGTCATTCGTTTAATTCACGGCATGGAAACCGAAATTATTAACCTTGGCAATGACATCCTCTTTGTTCGCGCTCCGGTGAACAACAAGAAGCTCTTTAATCGTACGTTGGGGGAAATTAACATCCGTGAACACACGAATGCTAACCTCATCTCAATTATGCGGAATGGCAAAGATGTCGTCTTTCCTTTGGGTCCGAATACCCAGATTCAACCAGGGGATGTAATTACAGCCGTGTGTCAATTAAGTGGCGTGAACCAGTATTTGCGCTACATTAATCCTAACGACAAGAATAAGTACAAAGCGAGTGAATAATTAGATCACCCAAGACCCCTCCAATCCTTATCGGTTTGCCGTTTTACTCGTGCCGCGCAGCGTGGTGTACTATGAGCAGTTGCAAAGGGGATTAGCGCTCCCTAACCAAGGGAGTTCGTCAGGCTCAGACAGCACTAACCAAACAGGCGCAATGTTTGGCTTGAAGGTGAAGGATGCAACCGTGGATAGTTCGAAGCAATCAACGGAAAGCTTAAAGGGCGAAGAATCGAGTTCCAGTTCCACCACATCTTCCACCTCCACCACCCAACGTGGGGGTTCGTCAGGGGACACCAAAGTCAAGGCGTTGCAGGTGGCGGTGAAAAAGAAATCGGACTCGGAGGACAATGGTCAGATCGAACTTGAAACCAACAACCTCGCCAACGCCCCGATTAAACGGGGCTCCAATAACAACCAGCAAGTCCAACTCAAGGCGGACGATTTTGGTACTTCCCCTTCCAGTTCGGAATCTGGTCAATCAGGCACCCCCACCCCCTGAACGCCGTGGCTTGCGACCGAGCAAATTCACAAGGACCTCCCCAAATGATCCGCCTCGATCCTGATTCTGTACGATGCGCCTTATGCGCGCAATCGTACCGCCATTGATCGCGTTGATCACTTGGATCCCAAGGTGATGACCGCGAACTATCCGCCCAGTTGAAGAACGCCCAAGTGAAACCACCACGGGTTGTGGGACTGAAAGGCGCGCGATGTCCTGCTCCAAACCACCGGGTTCTTCAACCCGCGCCGCCACCCCGAGTGGTTTGACCAGGGCCAAGCGGTCGCGGAGAATACCCAAACCGGGTTTGATACAGATGACACCGATAATAAAAAGCAGGGCTTTCGCAAACAAGGCGAACAATCCCCTGCCCCCATCGCCCTCCCGTTTGAAGCGTACTTCGCCAACATTGGCAACCTCACCTGGTTCGGGCAAGCGCTTTTGGTGTTTGGGATTTGTTTGTCTTAATTAACTAACAAATTAATAAATAGGTCTATGGATTAATTACCTCACCCCCTACACGCCCTTTACGACACCGCTCAATGGGGGGCTGGATGTCGTGCGCGCCGCCCATTTACACCCCTCATACGAACTCGTGGACTGAAAGCGGGTGGGACAGAACAAGTTGGTGGCGCTGGTCCGCTCAGCGTTGGTCAGGGTGAAGTTTCAAGATAGTTCGAGCCAACAAGGCACTGAGAGTAATGGCCAAGACCAAAATGCCCTTAGTTTTGATACCACCAAGTCCCAACAAGCCTTACAGCAAGCTGGCGATCAATCAGGAACTTCATCTAACTCCACACAAGAAGACTTCGCCAGCTACATCCTCATCTTTAAAGCCGCGCCCAGGGCCACGTGGGTATTTGAACGCAAGATTAAGTTGGCGTTGCCCTACGTTAAGCAGGAAAGTCAGGGTTCCGACGATCAAGGTTCCAATGGTAAGGGCTCCCTCTACAAAACCCTCCAAGACCTCCTCGTCGAACAACCCGTGACCCCTTACACCCCGAATGCGGGGTTAGTCCGGGTGAATGGGGTTGCTCAGGATACGGTTCATTTTGGTTCGGGTCAAGAATCGAGTTGGAATTCCCAACGTTCCCAAAAAGGCCTTAAAAACAACCCCGGACCCAAAGCCGTCACCGGCTTTAAGCTCGATAAGGGCCGCGCGTACCAGAAGTTAAATGAAGCTTGACCGGTGTATGAACCCCTGGATTCGACGAAGGAGGGGAAGGAGAAAAACGAATCGGAGTGGAAGTCTTCCGGGGCGAGTGAGGCAAAAAATGATGCCCCGTTGGTGGGATCAACCGGGAGTCAAATGGCTGCTGTTACAGACAATCAGCAATCAGGTGATAACAATGGATTGGTATCGCTTGCACAGCGCTCCACTACTGTTGCTGTCCAAAAAAGTGACTCTTCTGGTTCTCAAGGCCAAGGCACAACCGACAACAAATTCCAAAAATATTTAAACACCACCCAGGCCCTGCACCAGATGGGGGTGATAGTGCCATCTTTGGAAACGTGACCTGGTAAACCAAGTACTGGAATCGCTACCCGTGCTGCTGGTGGTGTTTCAGTCCAAGCAGCGACCCGGCAATCGACTTCTACAAATGAAGATTTGTCGAATGTAATAACACAGCTTTATCATACCTCCACGTCCCAACTCGCTTACTTAAATGGCCAGATAGTTGTGATGGGCTCAGACCGGGTACCGAGTTTGTGGTATTGGGTCGTGGATGAGCGAACCACGTCCGGGAGGGCGACTTGATGAGCCCACACCGAGCTCAACTGGGGCACCGACAAGCAGAAGCAGTTTGTCGAAAACCAGTTGGGGTTTAAAGATGACTCAAATTCGGATTCCAAAAATTCGAATTTGAAGGCCCAAGGCCTCACCCAACCCGCCTACCTCATCGCCGGTCTTGACGTTGTGGCCGACCACCTCGTCTTTGCGGCCTTCAAAGCGGGGGCAGTGGGGTATGATATGACGACTGATTCGAACGCTTCCACCAAAGACCAAGTGCTCGCCTGGTCGACCACGGCCGGATTGGACAGTGATGGGGGGTACAAGGCCTTGGTGGAAAACACGGCCGGATTGAACGGACCGATTAATGGCTTGTTTATCCTGTTAGACACCTTTGCCTATGTGACCCCGGTGAGTGGGATGAAAGGGGGGAGTAAGAATACGGAGGCAGTGCAAACCAAGTATCCCGTTAAGGATGATAGTAAGGCGAGTGCCAAAATTGCTTCCTTAATTAATGCCAGTCCACTCAACAGTTATGGGGATGATGGCGTGACCGTGTTTGATGCCCTGGGCCTTAACTTTAACTTTAAGTTGGACGAGGCGCGCTTGCCATCGCGCACCGACCAACTGCTTGTGTATGGGATTGTAAACGAAAGTGAACTGAAGTCCGCGCGCGAAAATGCCCAGTCGACATCCGATGATAATTCAAACACCAAAGTCAAGTGAACTAACACCGCCTCGCACTACCTCCCCGTGCCGTATTACTACAGTGCCAATTTCCCCGAAGCGGGTAACAGGAGAAGAGCGGAGCAGCGGAATGGGGTGATAACAATAAAAAAAGCCATCTACCCAGAGGTAAGAGGCAGACAGCTCCAAAATATTTTTGACGATTGGAAAACTAACAAAAGCCGTTAGTTTGATGTAGAGGCAAAACTATGAATTCACTAAATTTATTTAAATCAGCGGGAGCAGGCTCGTTTTTTCAACACAAGTCACTCTGCCTGCTCCCACTAAATAAATTGAGACAGATTTTCAAAGTAGCCAAAGAGTTTGAATATTTTTGCAATTTGGGTTTTTAATACCTTCTAACTATTAATAGGAACTTTTCTTATTAATAAGAACTTTTTTGAAAAAAGTTTTGAAGTTGTTTTGGTGTCTTTACTAACTTTAAGCAATTTAGTTAGTGTAAGCACAAAGACTCGTTATGTGTAATGTTTTTATTAATTTAGCAAAACAGCTGAACTTAGTTGGCAGCAGTGCTGCAAGTTTTAGTGACTTTTCAAACCTTGCAAAGAAAGGAGGTATTACTTAATGAAAGAGAAAATTCCGTTTTATAACGAAAAAGAATTCCATGACATGGTGAAAAAGACCAAAAAGGGCACTTTTTCAGGTTGATACATCATTAATCCAGAAAACAACAGTGTGGAATTCTCTGGCAAATTCAACAGACAATTTAAACTTAACAAACCGGTTATACCAGTGAACACGGAATACGTAACGCGTAAAGAGTTCAACGAGTACAAAAACTCAAACAACCAACGACTTACAAAGATTGAAACCACTTTGGCCGCCCAAGGTGAACAAATCAACAAATTGACTCAAACTGTTGAAAAACAGGGCGAGCAAATCAATCAATTAGTTCAAGTTGTGCTTCTTCACGGCGAGCAAATTAACAAACTGACTCAAACTGTTGAAAAACAGGGCGAGCAAATTAGAGAACTTCAAGTAGAGCAAAAATTTCAGGGTGAACAAATAAAAGCTCAAGGAAAAACGCTTAAGTCGATCCTACAAGCGCTTGGAGGAATAAATAAACGCTTGGACAAAATTGATCCACCTAAATAGTTTTTGGGATAATAACAAAAGTGCCAGCAGTTAATCTTAAAGATTAATTGCTGGTATTTTCATTCATTTGAAATGAACAAAAAAGCCATCCTATCAAAGGTGAAAGATGGACGGCTAATGAATTGAAATCAATTACTTAATTAAATATTCCAAGTAAATAAAGAGATTAATATTTGATGAGTAGCCTTCTGTATTATTAGCAAAGGTACTTAAAAATTTGTTCACACTTTTTTGACTAACATCATCTTTGCCTTTTAACGAAAATTTAAGTGAAGCGCTAAACTCAATTCACTCTAAATTTCTTGTAGTTTCTTTCCCACCAATAGTTGTAGAGTTGTCTTCTTCAGTTTTAAAGTTTGCAATTGTAAATGATTTTTTGGCATCAGGTTTGATTTTAAGGAAAAGATCAAAATAATGCTTATCACCTTTCTTAGCAGTGAAACCACCTGAAGAATAATCTCCAAATCAATTAAAGGTATAGCTTAACTTAGCCTCAACTGACAAATCCTTAAAACTTTGGTATGTCATCTGGAAATGTTCAATCTTTCGCTCCATCGTAAAAGGATCCTTGCTTGCGGGATAAATTACATCGTCAAATCAAAATCTGGAATACCTAATTTACCCTCTTCAAAACTCTGTTTTCAAGCATCTAACAACAATTTTGTTGTATCTCTCGGATTAACTTCGTAAGACTTTTTTAAAGAAGCTATTATGTTCTTTTTGCCTTCTTTAGTTTTAATTGCTTTAGACAAATCAATGTCTTTACCAGTGGTTTCTTTAATAAGATTTCTAAGATCTGCTTGGATACCAGTGCAAGCACTTAACAATGTTCCACATAATAAAAATAGAGAACTAAAGGAAATCCTTGACAACAATCTCTTTTTGCGCATAAACAGTAAAAATAACTCTTTGATTTTGCTTTTTTTTGGAAAATCCTTTTGAATAAGAGGAAACTAATTGAAATTTCGGGTTTAACATTAGTTAAAAAATGAAATTTCTAATTATTTATATAACAACTATCTACTGATAAACGTCTCGATCTGAGTAGTTCTAATAATTACCAATTGTTGGTATTTTTATTCATTTAATGCTTTCTAAAAACTTTTTTTTGCAATTTTGTTTGCACTTCTCAATAAGCCGGTCAATTTATTTAGTGGAGGAACAAAATGCAAATCTTTTATTTTTTGAATTGATCAGCAAAGCGTCTGATCCCTGTTGGCAACCGAGTTGCAAGTGTTAGTTATTTTTTAAAAAATCAAAAGAAAAAAGAAAGGAACATTTAAACATGAAAAAAGTTAAAGCATTAAGGAATCTTTTTCTAGAAAAAGCCAGTGAAAAACTTGAATACCCCAAAATTGCTTCAATGGTAGCTTACTTTGATCCAAAGGAAGATGGTACTTGTGAATTCATTATGAAAATTAAAACTGTCAATGAAGTTGTTGACACTGAGACGACAAATAAAGAAAAGCCTTTAGGCACTTGAAAGAGTTAGTTGATTTTTTGGTCTAAAAAGACATAAATTTACCAACGGTAAGTGTTAAAACTTGCTGTTGGTATTTTTATTTCTCCCCCACGCTTTCCCGTTTCTCCACCGGGTTCAACCTCGCGGGGGCACTCCTTGACCAGGTGTTGGATTATATTCCCTGGATTGGGAATGGGTACAGGTATGGGAATGGATATAAGGGGGTCGCTGGAGCGGGTCACAGCGCCACCAACGGCTCGCCGGCGGGCAACACAAGTTCCACCACGCAGTCCAATGATGTTGCACCCACTTTTAGCAATGTCGGCGTTGGTCTCAAAGCGAATGTCCAGGGCACGCTTGGTGACAGTCAGACGACGACTGTGGGTGGTCATCAATGACCCACCCTCGACCCAGCCAACCTCCAGCTCTGAACCGGCGCGGAGTGAAGGAATGATTCAAAGACGCAAAATAATACTACGACCAACGAAAACCACACCAAGTTCGCGAGTGCTACGGGCAGTGGTCAGCAGCAGGGTTCCACAACAACTACCTCCGCCGGCAACCCCGACTCGTTAAAGCAGGATAAGGCTGATAAAAGTGGTAATTCGATCAGTGTTCAAGAAGCCACATCGGGCGACAACTTGACCAACTACACCAACCTCCCCCCCAACCTCACCCCCACATCCGACTGACCGAACGCTTTGAGCTTCACCAACAAGAACAACGCCCAGCGCGCCCAGCTGTTCCTCCGCGGCCTGTTGGGCAGCATCCCGGTGTTGGTGAATAATTAATTTAAGGTCAAATAAAGCAATAAAAAAAGGATCCAAACAGATCCTTTTTCAAATAGAACGCTGCCAATAAGCCATGTTCTGTTTTAAAGTCAGTTATTTATCTACAGTAATCCTGTCATTACTTTAATTATTTTTCTTAAAGTAACATTCCCTTACCAAAATTTAGGTTTCTTGCTTGTGGAGTTTACCGCGTTTCATACTTAGTTTTCACTAAGCTCGTCTCTGTGGTACTTTACGGACATCATCATAGTCAAAGACCTTAGACTAGTCCAGTCGTGACGTCTATTAGCGTCCTAAATCTTATTTGTTCAATTTACACAAACACTACTTGCGTTCCAGCAAGTGCAAGCATGGACTTTCCTCTACTCATTGAGCAGCAACTGACCGACAGCGGCTAAAATTATAATTTGAGCGTTGGGGATGTAGAGGTTTTGACATAATGTTGAAAGGAAAACAGTTGCAGTGGGGTATGCCCCTTACAGCTCTAGGTATAATAACCGACAAAAATAACGACGAAGTTTTGGTAGATCCAATGTTGATCGCTAACCAACAAGCAAGTATCAACTACGCTTTCGCTTAGAACATACTAAAGCTACACGAATTGAATCGCCATAGTTTGGTTCGTGTCACAGTTTATGGCTCGGGGTTAACTGGTTCAACTTAATCCTTAAATTATGAACTTATCGTTTACTTGTTTGTCTTATGATCTAAAGTAAGCGAGACATTAAAACATAAGACTAAACTGTAGAAGCTGTTTTACCAATCCTTTATGGAAACGGGTTCGATTCCCGTCATCTCCACCATTAACAACGCAAGTTGACCAAACCTTCCTTCGGGAAGGTTTTTTATTGCTTACGTTTGCGACAAAAAATGGCCTTAGCGCGTTGCTTGTTTCAGTTAAAGGCAAACAGTCCTAAAAAAGGCAATTCATACACCACAAGCGTAATACATAAAGCGGTGGCAAACGCTCCTAGTTGGTTTAAGCGCGAACTAAAAACGTTGTCTTTGTGAGAAATCCGAAAGAACAGTGACAGGTTAGGATTACTTTTTAGTTGCGTTGTCGTACGCGGGAAAAAGTGTTGGTCCGATAACACCAAAAAGAGCAACATCAACGCAATTCAAAAGCAATAAGCGACCACACTAAAGTAAAAACCAGTACTAAAAATGACCCGTTCTCACTTAGTGATTTGACTCTTAATAAACAACGAAATTGCTCAGCGGTAACCCAAAAAGATCATTAAAAACAAGCTAAAGAGGCCCAACGCTAGAATGGCAATACCAAAGCTGCTAATCGAATACACAAAGTTAGCGTTAAAGATGTCAGCTGGCTTTTTGACATTTCAAAACAAGTAAAAACCACTAATGCCCTGGTTTTGATCGTTTAAGGTAAACTGCTCGGTGTGAAAGATACCCACCAAGAACATCACTGCTGTCACAAAAAAAGACAGCAAGAACAAAAAGTAAGCAGCGGTATCTAACAGACCAAAACGGCTGTAGAAGAGGTACTGACTGTGTTTGGAAGCAACTTCGTATTCAACGGTATTAATTTCGGTGAGTTTATCGTTGATCTTACTCATCCAACCACCGCCGCTAGCAACGTTCTTTTTCTTGTTAGTCTTAGAAGGCACGCGGAATCTTGGCTGATAACCACACACTCTTGTGGTCTTTAACTAACACCATGTCCTCAATGCGGATGCCACCAACACTAGGGATATAAATACCCGGTTCAATCGTAATGACAGCATTTTCACACAACAACTTGTTATAGGACGTTGATACGTTTGGCATTTCGTGAATGTCAAGTCCAACACCATGACCAGTGCTGTGAACAAAGTAATCTTTGAACTCCGATGCTTCAATAATGTCACGGCACACCTTGTCAACTTCCGCACCGGTGAGTTGGGTATTAGCAGCGTTAATGCCGGCCATATTGGCTTCATCAACCTTTTTATAGGCCTTGAGCAACACTTCATTATTAGGTTTTTTACCAACTAAGAAGGTGCGGGTAATATCGGAACAGTAACCGTTGTAAATGGTGCCAAAGTCACAGGTTACAAAATCACCTGATTTTACCTTTAACTTGGAAGGCTTGTGGTGTGGATTAGCACCGTTCTTACCAGTAGCTACAATAGGGTCAAACGAGTTTTTTGCCCCACCAGCTTTGACCAATTGGTCAGTAATCCACTGCGCAATGAACAACTCCGTCATGCCTGGTTGGATAAAGCGCTTGAGTTTTACCGCTACTTTGCGGGTAATTTCCACTACCTTTTCAATCGCTAAGATTTCACTCGGGAGCTTTTGTCGGCGAATTTCCTGGGCGTTAATGACCGTGTACTGCGCACACAACTCCTTAATGAAATTTTGGTAGTTAAAAGTGAGGTAATCCCCTTCAATCAAGAGGTGTTTCACACCCACTTGTTCACATAATGCCTTTACTTGTTTATAAGTGGTAAATAATTCCACTTTGACTAGGGGATCAATTGCCGTTTTAGCGGCTTCAAAGTAACGCCCGTCAATAAAGAGGTTAACCCTTTGCTTGTGAACCACTAACCAACCAGCACTGGAGGGAAAACCAGTAAGCCAAAAGCGGTTTTGGTCTGAGCCAATTAAGATGGCATCAGCTTTATTGTCTTGGAGCAGTTTGTGTAAAACTGCTAGCTTTTGTTGCAGTTCGTTATGCATTATTTTCGTTTTACTTCCTTGTGTACTACTACCTTGCGACAACGCGAACAAAACTTGTTGAGGGCAAGTTTTTCAGGGTTTTTCTTGACGTTTTTAAACGTTAGATAGTTAATTTCACGACAATCATTACATCCTAGCCGTGTGCTTCTCTTAACAGCCATAAAGTATATAGATTCTAATTATTTATTTTTATCGAGTAATAAACTGATCGAAAACGCATCTATTCCGGTATGCGCAATAATGGCACTTGTGATAAACGAGCTTTCGATTTCACGTTGTAAAGTGATTTTGTTAGTGTGGAGAAAATCCAAAATTAAAGCTTTAACTTCCTTGGCCTTAACTGGGGTAAAAGCATTGCAAAAACCAATCCGTTTGATCTTAAAGTTATCACCAAATTTTGATTTGACAAAGGTAAAGATTTTCTCCAATGCTTGGACAAAGGTAAAGGCCTTGCCGCTCAAAGTATTTACCCCTTTATCAAAGAAGATAATCGGTTTAACCCGTAAAAGTGTGGCAATAATTTTCTTTAATCCAGAAATGCGCCCACCTTTACGTAGCTGCACTAAGTTTTTTACAGTCACAGCTGTAAAAATACTTTGTTTGTGCTGGTCAACCACTTCAGCAATTGCTTTAGTTGGGCAGTTAGTGTCAGTTAATGCCTTCACCTCTTGTACTAACCATTTCAGGGAAATGGCAATGTCACTTGTTTCCAAGACCACAAACTCTTTTTTGGGCGTACTGAGTGGCTTAACCGCTTGGACAATCATATCATACGTACCACTCAAACCCTTACTGAGAGGTAAAAAAACAAAGCGATCGTACTTGTCTTTAATGGCTTCAATTAACTCAATTAAGTCAGCTTGGCGTGGTAGTGATGTGGAAATGTTTAACCCTTGGGGGTGTTCCTTCAACAACTGGTATACTCGATCATAGTCAATGTCCTTACCATCGCGGTAAGTGTCTTGACCGTTAATAATGACCTGTAACGGTAACACGTAAACGTCTTGGATCTCTCCTTCCTTAATTGAGGCAGTCGAGTCAGTAATTATCGCGGTCTTCATGCGCTCTATTTGTCCTTATTTCGCTTTATATTATCTAGAAACTCCCCAATGTAATCAAAAAAAAGTTTGGGTGCATAGTAGTGGGGTGAATGTCCTACACCATCAATAATTTTGAAAATGATCGTTTCGCTGTGTTTAGCTAAGTATTCCACAGAAGCCTTTGTGGGGGTGACAATATCATTGGAACCGAGAACCACTAAGGTGGGTTTGTCCTTAATTGCGTTGTAACCCGCTTCGAGATAATCATTACCATACTTGGCGTTTTGCACCATGTCACTGTAAAGTGTTTTAAAGTTGACTCGCTTCTTAAAAGCACCAACAAAGAAGTTAACAATCGACTTCTTATCATCGGTGTGTTCTATGAAGTCCTGCTGGTTTTTAGGACTCCTGGTAAACAAGGTATCCAAGATCCGTTTTTTACTTACAACAAACGATGTTTGATTCATCGGTGCTACCAACACTAAGGCCTTAATGCGTTCACGCAATACAGCATTGACCAACACCGCAATCGCCCCGCCCATACTGTGCCCTACCAATACCACCTTTTTCAAGCGCTTTTGGATGATAAAGTCACACACCAATTCAACATAGTGTTTCAACTTCAATTCATCAACACTGTTACTCTGATTATTGCCGTGTCCCGGAAAATTAAAGGCAAAGAACGACCAGCGCTTTTTTTCAAACAGTTTAAACACGTGTTTAAAGCTGGAATACTCACTGCCAAAACCATGGAGAAAGATAAAGTTATGGCGCTTACGGTGTGGTTTAAAGGCAAAGATTGATTGAAAGGGATCGCTCTTACTGCTTGCCATTGATCTTAGCTAGGCGTTCAGCGTACGCTTGGATCCGTTCCTTTTTGGATAACTTGCCCAAAGCATCTTCCTTGTTTTCGATCACAATATCACGTTTTTTTAGCCGTGGTTTCTTTTCGGGTGGCACTGGAATTTCCTTTTCCACAATAATGACCTCTTTAACAGTTTCGTCTTGCTTTGTTTTAATTTCGTGGGTTTGACTCGAGTTGAGTTCGTTGAAGTATTGCAACTCGTGTACTAAGGCTAAGTTTTGTTGCAGTAAGCGTTGTTTTTCTAGCTGCACTTGGTTAAATTGTTCTAACAATTCATCGCTTACCGGTGGAGTTGCTTGGAGGTTATTGTAAGCAGCTTGGAGTTGGGTTAGCTCTGCATGGGTTTGACTTAAACTTTGGTGTGTGTTGTGTAACTTTTGCGCTAACTGTTGTTTTTCCAAGTTGACCTGGTTTAAAACTTGCAACTCTTGCTTGAGCTTGTTGTGATTACTTTGTAACAACTCGTAAGCGGAGAGCAAGTCCTGGTACTGGGCGTTGGCCTCATTAAGGTTAGTAATTTGAATTTCGTTTAATTCTGCTTTGACCTTGTTGTAACTCTCTTGCAGTAACTCATGCTCCGCTTGGATTTGGGCTAAATTACTGTGTTCATGTTGCATTTGCTCTTTTTGTTGAGCATATTTGTCTTGCAACAAGTTATGCTCTGTATTGAGGTTCTCCCAGTTAGTCTTTACCCGGTTTAAATCATGCAACTCATTTTGAATGTTACTGCATTTATCAGTTAATTCCTCGTTGTTTTGCTTAAAGAGTGCTAACTGTTGGTGGGCAGCATCAAGTTCATTTTGTGCTTGATGGAATTGTTGCTGGGTGTGATTAGCTTCATCTTGCAATTTGTTGTAACTCTCTTGCAACAGCTCGTGCTGCGTTTGGAGCTGGGTTAAGTTTTCATCACTCTGTTGCAAGTGGTTTAACTGGGTTTCGCTAGCCATTAGTTTGTCAGTTAGGCTTTGGTTTTGTTGTTTGAAGGTAGCTAACTGTTCTTGGGTTAAGCTAAGGTCAAACTGGGTTTGTTCTAGCTGTTGCTTTGTGAGTGTTAGCGTTTTGCTGTCAAACGCAGTTTTTTCCTTGTGGGTAAATTCTAAAACATTTAGTTGGTTTTGTAGTTCACTGTTAGTAATACCCAAGGCCTGGACGTTTTCCTCAGCTGCTTGTAAGCTTGCAAGTAAACTGCTGTTTTTTTGTTCTAGTACCACTTTTTGCTGTTCAAAACCTTGTGCTGCGAGACCTAACTCAAAGTTAGTGGCCTTAACTTGGTCTAACTCTTTTACTAAAGCTTCGTTGTGGCGTTGTAATTGTGCTAACTGTTCCTGGTGGTGTTGGTGTGCTAGGTGTTGTTCGTGTTCCAATTCCTTTAACTTAGCATCACTGTCAGCACGCATTAACTCATACTGCTTTAAAAGATTACGGTACTTGGTGTTTTCATCGTTTAACTTAGTTTCACGTAGCTGTTTAATTTCGGTTTTGAGGAAGTTGTTTTCACGCTCAATTAGGCTTAAGCGTTGGTTAGTTTGCTTTAAGCTAATTACTTCCGTTTCCAAACTGTTAATTTCACTGCGTAACGACGGAATTAAAGCCAGTTGTTCCTGGTGTTTTTTGGAATCAAACTTGTACTGTTCGATTTGGAGTGTTAAGCTGTGTTTGTCTTGTTGGAGTTGTTCAAGCTGTTTTTCCAAGACAAAGTTTAACTGGTTAGCTTCCGGTAGGTTGTTTTGTAAATGCTGTTTAAGCGTACGGTTTTCCTCTTCCAAACGTGCTAAGAGTAAGCTGTTGTTTTGGCTCTTAAACAGCTTTTGTTGCAGTTCATTCAACTCCTGAGTGAGGTTAGTATTTTGGTTTTGCAACAGTTCAATCCGTTCCTGTAACACCGGACTTTGGTCGTACTGAAGTGGCTTTGCTAACTGCTCTTCTAGCTGGATTTGTAAAGCAGCGTTGTGCTTCAAGGCCTTATCTAACTGTTCTTGGAGCAGGGTCATTTCAAAACTCACGGTAGGTTGTACTGGCACTACATCTTGAACAGGCAGTAGCTTGTATTCTTCCACACCCATGTAAGTTACAGGAGGTGTTTCTAGTTGAGCTTGGCGTAGTTCAATTTGGGCTTGCAATTGTTGTAACTGCGCTTGTTTGGTGGCAATTTCCTTGTCTAAAACGGCACTTTGATGAGCGCTGTCTTCAGCGGGTTTTATAACCACTGTTTGGCTCAAAGCATTAGTTAAATTAGCCTTGTCCACCGTTGTAATCGAGAAGCTATGTTCAGCTGCAGGTGATGTCACAACAACAGGTGTAACTGGTGCAGACTCGTTGACTAATGTATGGGTGAGTGGTACTGTTACTAATTCATCGACTAACTGGGTTTGGTCAAGTGCAAAGTTAGTTGGTGCTGCTTTTGGTTGTAACGGTGCTGGAGTTACAGTGTGAGTTAAAACTGGCACTTCCACTAGTTCGTCAAGCAGTTGTGTTTGGTCTAACGGAACAGTTGTGGTAGTTGGTGCAACTGTGTCAAATACATGAGGTACCTTCATTTCCGGGTACACCAAAATGGCATTACTGTCAATCGGACTGCTAAAGGTCAAGCTAACAGTAGGATCAAGTGCTGCTTGGGGTAAAGCAGTTTGGGCTTGAACATCCTGTTCCACCATTTCGACATAGTCATCCAACAACATAGTTTCACTAACAGCTTTTGTGGCTGCTGGTTCTGTTGCCACACTAAACTTATGGGTATGGTTTGATGAAACTTGTTCTGATGCGTTTTTGATTTGTTGGGTTAGTTCAATGTATTCGTTGACAAGCACACTTTCATCGACTTGTTGGGTTGAAGGAGTATTAAAGACCTCTTCAAACAGTACTTCCTTAGTGGCAAAGGATATAGGTATTGGTGTTGTTTCTAACTCTTTGGTGTGCACGTATTCATCGACTAGTTGAGTTTCATCTAACTGGGGCTTGGTCGTTTGTTCGGTAATACTAGAGGGGGTAATTTCACAAAAACCAGCCTTAATTTCCTTTGTTAGTACTTCCTGGCCTTCTTGTTCTAAAAACTCCATTGTTAGATCAACGGTTTGCGGTTTATGAACTTGGAGTTGGTTAGCGAAGTGGTTTAACACATCAACATGGGGTTGGCTTTTGACCACACCCATATCTACCATAAAGTGAAAAAAATTGCGAATTGATTGTTCTTTCTCTAACTGATCTAATTCGCCAAAGAAACTACGTTCTACAATGTTACGTTCTAGGGGAACTGTTATTGTTTCCCTTGTTGGTTCTAGGGTAATGTTACGGGTTTTGGGCATGAGTTGCTTGAGCAAACTTTGTTCGTTTAAGTTTTCGTCAAAATCGTGGATACCTAGCTCTTTTTCTGCTGTCATGAATGGCACAGACCTCATCTAGTTAAGTATATTATCTTAGGTTTTTGCGACACCTCTTATCCGTGCATTTTTGGACTTGAAATAAAGAGTAATAGGTACAGCACTAAAACCAAAGCTTTCCCTAATCTTGTTCTCTAAAAAGCGCGCATAGGAAAAGTGGACAAACTTAGGGTCATTACAGAACAGTACAAAGTGCGGCGTTTGACTGTGAGTTTGCACCGCATAAGTTACCTGTAACCTCCCACCCTTAAACAGGGGTGCTTGGTTAAACAGTTGTGCTTGTTGGATGACATCGTTTAACAGGGAAGTTGAAATCTTTTTGGTGAGCTGTTCCTGGATAATTTGGAGCTGTTCAAAGATGGTATGTAAGCGCTTGTTGTTTTTGACACTAATAAAAACAATGGGAGCAAAGCTGAGGTGTTGAAACTGACTTTGTAACTGCTTTTTAAACTTAGCCATCGTGTTGCTGTCCTTCTGTACTAAGTCTCATTTGTTGACCAAAATAATCACCGGAATTAAAGCGGCTTGGGCTAACCCACCGATAATCTCGTCCTGTTCACTCAACGGTTTGGAACCATCAACCATTAACAGAATAATGTTGCTCCTAGCGATTGCTAACTGGGTTTTCCCATACGAGGCCGCTTCAATTCCGGGGGCTATTTTCCCCTTACGGCGAATCCCAGCCGTATCAATTAAAAGGTAGTTTTGCCCATTAACCCTTAAGGGAATATCAATTGCATCACGGGTAGTACCACTCTCATCGGACACTAACACCCGGTTTTGCTGCACCAGTTGGTTAATTAAAGAACTCTTGCCAACATTGGGTTTACCAATTACACAAAAGCGCACTTCGGGATTGGTTTCACTACTACCATGGTAAGGGGGGAGTTGGTTGTCCTTAACTAGTCGATCCATTAGGTTACCAATGCCAATGCCATGGCTAGCACTCACACAAACGGGTTGGCCAAACCCAAAGCTGTAGAACTGTTGTAGGTTGGGTTCATAAGCATCTGGTTTGAGGTTTTCTGATTTATTGACGACCAACAAAATCGGTTTGTCCTTAATCTTTTTCAATACCTTAGCTACATAAAAGTCATCACTGCTAATCTGTTCTTGGTAAGACACCAAGAAGATAATCGCATTAGCTTGACTTAAGGCTGCTCGAACTTGGACTTCAATTTGTTGTTGGAGCGGGGTCTTTTGGGAAATTAATCCCCCAGTATCAATAAAGGCAATTTGCCGCGTTAACCATTCACCAACACCATAAATGCGGTCCCTTGTGGTATTGGGCGTATCGGAAATAATCGCGTAGGGTTTTTGGATTAAGCGATTGAACAAACTCGATTTGCCAACGTTAGGTCGGCCGATAATGGCAACAGTAAACACCTTAAAGTTTAAAAACTGTACGCGCTTGTTGCACCATTAACTCGACTACTTGCTGAAAAGTAAGGTTAGAACTGTCGATGTAAATCGCATCGGGGGCTTGCACTAAGGGATCAGCAGTTCTGGAAGTATCCACCGCATCACGGTGCTTAATTTCCGCTACTAGTTCCTGAAAGGCCTTGTCACTGTCATATGCTATGCCAAGATCTTGGGCGCGTCGTTGCGCTCTTATTTCCGGTTTAGCATCCAAGAAGTACTTTAACTGCGCGTCCTTCAATACAACGGTACCAATGTCGCGTCCATCCATCACAATGTTGGTGTTTTTAGCTAACGCTTGTTGGCGCAATACCGCCATCTTACGCACTTCGGGATTGGTTGCTAAATTGCTCGCTAGTTGGGCAATTTCCTGGTTTAGGATAACCTCTGAGATATCAGCGTTGTTATAAAACACCTTTTCGTGTTCAAAGCGCCAGTTAATTTGGTTAATCACCTTTAACAAAAGGTTGACATCCAAGCGGTTGACCTGCATCACGTAAGCGAAGGCCCGGTACATTTTGCCAGTGGAAAAGTACTCAAAACCGAGCTGTCGGGCCACTGTTTTAGCAACACTAGATTTACCAGAACTACTAGGACCGTCAATGGCAATTTGAAAATACATAAATTTAACTTAATGGGTGTAAGCAATTAAAGGGAAGTGAATAAGAGGGTTAGTAAAACAACCAAACCCACTAAACTACTAATAAAGAATACTAATAAACTGAGCAAAATCCGCTTAAATAACGGGTTGTAACTGCTCGCTAAGGGCTGGCTTAAATCAGTGGTGGTAAAGCTCACTTTTTCGTTAGTGAGTGCCATTAGTGCCTTATTATAGTTCCCCAATTGCACTACGAAAGTTTGCGTTTGCTGAATAAACTGTTGTTCGTTGTCCTCAAAAAAGCGCTTTAGGTTATCTATTTTATCCATCCCCCGGTCATCAGTAATCCAACTTTCATAACCAGTCGCGCTATCGTGGTGTTTAATTTGTAACCACAAGGCATCAGCTTGGGTGGCGATGGCTTGAAAATCCGATGTTTGGAAGTTGTTAATGAGAGTTTGCAGTGATTGTAAGACCTGATTGGGTTCACTTTTGCGTTTCACCGGATTAGCACTCAATTCACTAAAAGGCTGGTAGTGCGACAGAATTTTCGGGCTCAATTCGTCTAGTTCTACTTTTCAACTAGCGACCTGTTTTTGCGCTTTCAGTAACGCTTTTTGAACAGTCGTATTATTGTCATCGTTCTGACGGTAAAAGCGAAACTTCTCTAAGCGTCCCATTGCTACAGTGCATTATGCACTACTGTTTGGATGTCATCATCCTCTTCACACGCATTTAAAAAGCGGTTTAAGAGGGTGTGTTGTTCGGCGTTAAGTTCCACCGTTAACAGCGGAATTAAGCGCAACTCACTCGCTTCAATCGTAAAACTGTTTTTAACGAGTAAATCCTTCAACGCAAAGTAACTCGGTGAATCGACCACTACTTCAAAGCTGTCATCATCTTCATTGAGATCTAACAAATTAATGTTGTTACTAATTAACAGTTCAATTAACTCGTCTTGGGTAAAGTTATTGTGTTTAACTAGCGCAATGCCCTTTTCCTCAAAGTTAATTTTAACACTGTTGGGCTTGGCTAGCTGTGCCTTTAATTTAGCAATGTAACCGTTTAAACTACTCAACAACCGGTTCGGATTATCGGTTAGACCAAAGACAATGATGCCAACACCATTGGGACCAAAGATCTCATAGCAAAACTCGCTTAGTTTTGTGGGATCCTTTTCACTGCCATGGATGTTGCGTTTAATTGAATCCGCACTTAAACCATGAGCTAACGCTAGTTCGACTGCCACCTTTAGCTGGGGGTTGGCATCAATGTTACTGCCACCTTTTTTGACAGCAGCAGCGATCCGCTTCGCTAACTTTTGTAGTTGCTTCGCGTTGGATTGTTGTTTTTTGTTTGTTTGACTGGCAATTAAATGCTTCCGCGGCATGAATTAAAACTCATTAGCAATTGCGTCCACTTGTTTAGCGAACATGTCCACTACTTCACTCTTACTTTTGTCCTTGTAAAGTAGCTTGGTACCATCAATGATTACACTAGCAAAACTAGCGACTCCAGCAAAACCAAACAGACCGCGTAGACTGTTGACATGATCGCCCCATGGGTATCAACCTAACGGTGCGCCTTGGGAACCCAAAACGACTACCTTGAGGTTACCCAACATTCCTGATGATGCGCCCTTAGTGACATACTTATCCTTAAAGGTTTTGTTTGCCACAAAGACATGGTCAATAAAGTTCTTTAACGTTGCGGGGTAGTTAAAGTTGGTCATTGGCGCCAAGATAATAACACCGTGCGCTGCTTTGAGCGCATCAATGTACTTATCGGAGTTTTCTACAGAAAAGAAAGTTCCAGCGTTTTGGGTGTTATAGGAAATTTGTCCCACTGGTAGTTCGTTGAGGTTCCAAGTGGAGAGCTCTACGTCCTTGTTTTTAGCTTGAAAGGTAGCTAAAAAGCGTTCTAACAATAAATGGGTGTAAGAACCACTAGGTGATACAGAAGCATCAATAATAATTACCTTCTTCATACTGAACTAATTGTTATTGTTTAAATTCTACTCAGCTTTAGTTAATTTAGCGATTAAATGATTGACTTCCGCTAATTTTTTTTGATATTCCTCTAACTTTAAAAGCTCCGCCTTAACTTTGTGCGGTGGTGCTTTTTTTAGAAAGGCAGGGTTAGTGGTAATCGCTTGACTACGTTGGACTTCCGCTAAATAAAAGCTCCGTTGTTTTTCTAAGTGGGCTAAGTTGTTGGCTGCATTGTCAACGATTTTGATTTGGAAGCCTGGAGTTTTGTTAACTTCTATCCTAAGATCGACCCAGTTAAAGTGGAAGTAGTTTTGCACTACAGGAGCAGCTTTACCACTTAAAACAACGGCTAAATTCTGGTTAGCTACTAGTTGGTTGTTGGCCTTGTAGTGGCGCAAGCTGGACACGGCTTCAACAACATCAGCAAACAGTTTCGGTGGTTTAACTGCTTTGGCAGTTGGCCAACTTGCTTGCATGACACTGCCACTGTGAAACTGTTGGTAGATCCGTTCCGCCAAGAAGGGAGTAACGGTACTTAGCATAACGACCGCCGTACTTAAAACAGTTTTAGCAGTTGTGTACAGCTGGGGTAAAAGTGCCGTGTCCTCTTGCTTAATCGTTTCAATGAAGGTATTGCAAAAATCATTTCAAATAAAGTTGACTAACGCTTGGTTGGCCAAAGCTAACTGGTACTTGTCTAATAACTTGGTGGTTTGGACAATGAGTTGTTTCAATTTAGCCCAGATCCAGCGCTCACTCAATGTAGAGGGTAAGTGAGTACTTGGTACAGTTTTGGCTTGTTCTCCTAACTGCAACACGTACTTGGTCACATTCCACAACTTGTTCAAGAAGTTAGCGGCACTCTTTAACTTCTGTTCGTTGAAGATTAAATCATCACCCGGAGTGTGATTGGAAGTTAAGAATAAGCGCGTGGCATCGGCCCCATAATCACGGATTAAGTCCATTGGGTTAATCCCGTTGTTTAGGGACTTGGACATTTTGCGGTTTTGGGCATCGCGGACTAGACCGTGAATTAACACTTGTTTAAAAGGTAACTGCTTGGTTTGAAAGTACGAGTTAAAGGTCATCCGTAAGACCCAAAAGAAGAGGATGTCATACCCGGTTACCAAAAGATCAGTTGGCACAAAGTGCTTGTTTTTGTGTCAGTCTAAACAAATTAACGGTCACAGTGATGAGGAAAATCAGGTATCGAGTACATCGGTGGACTGGGTGTAGTTTTGCTTATCAGTTGGTGCAGTACTGCCCACATGCAACGCACCACTTTGTTTATGTGTTCACACCGGAATAGGGTGACCCCAAATTAATTGGCGCGATATGCACCACGGTTTGAGCTGACTCAACCACCTTGTTACCTGTTTGTTAAAACGCTTGGGAATTAACTCGGGGTATTTCTTAATGGCGAGTGCTTTTTTGAGTTTCGGTAAATCGACAAACCACTGCTTGGATAATAACGGTTCTACGACCGTGTCACTGCGCTCGGAAAAACCAACGTTACTGGTTAGTGGCATAGTTTTCACTAGTAGTTTTTGCGTTTGTAAAAACTCGACTACCTGCTGACGTGCTGCACTTACGGTTAAACCAGTAAATTGCTTAGCGTGCTCATTTAACAAACCATTGTGGTCAATGCAACTTACAAACGGTAAACGGTACTTTTCGTTTAAGGCAAAGTCGTTAAAGTCATGGGCGGGGGTGCACTTCAACACACCGGTGCCAAACTTCTTGTCGACATAACTATCGGTGAACACTGGCATTAACGCACCAGTGAGCGGATTAACTACCTTTTGTTTGACAAAGCTGTGGTAGTGCTTGTCTTTGGGATGGACAAACAAACACACATCAACAAAGATCGTTTCTGGTCTAGTAGTTGCCACCACTAAACGTTTTTTGGGATTATTAGCTAACTTATAAGCAATGTAATACAGCTGTTGGTCAACTGGTTTATTAATGACTTCAATGTTCGAGATGGCAGTGTTTAGCTTGGTGTCCCAGTTAACCAAGGTTTCAGCTTGGTAGATAAAGCCCTGTTCAAACAACTGGATGAAACTATCGTTGACAATTTGACTCGCTTTTTTGGACAGGGTAAAGTTAACCTGGTTTCAGTTTAAGGATGCCCCTAAACTTTTGATCTGACTTTGAATGGTGTCCCCTTGTGTTAAGGCCCAATCCATAATCATTTTGACCTTTTGTTTACGCGGCGCTTGGAAGTACTCGGGATTAGTTTCCCGTGCTAACTTTTCGTATTTGGTTTGCGTAGCAATCCCCGCATGGTCAAATCCGGGAATCCAGTTCACGCCATAACCCCGCAGTCGCTTAAAGCGCATAATTTGGTCAGTGATACTCACTTCAAAGGCATGACCAATGTGCAGCGTACCCGTTAAGTTTGGTGGGGGGAGAATGGCAGTGAAGGGTACCTTGTTTTTTTGTGGTTTAAAAAAACTAGCACTGCTTCAACTATCATAAAGACCAGTGCTCACTGTTTTGAAGTCGTATTGACCTTGAAAACTAAATTGCTTGTCCATCTGCTTTAAATAACTCAAAGATTTGTTTGTGCATTACTTGGAGGTTATACCCGGTTTTGGCACTAACAAATAAACACTTATTAGGATTGACTTTTAAAAAGTGTGCCAGTTTATTCTGGGTATGAAAGCGCTCGCTTTGGTTGAGCTTATCAAACTTATTGGCTATCACCAAAAAATTTAACCCCGTTTGGAGAATGGTTTTGACCACTTCCTGGTCCTGAACGGTCACTACACCGCTGTCAATCACTAGTACCACACCAGTTAAGTTTTGGCGAAAATTGAGGAACTGCGTTAATAAATTAGTAATTGCTTCCTTTTGCACTTTACTCAGTTTAGCAAAGCCATAACCTGGTAAATCGACAAAGCGTTTACGGTTGTATTCAAAGAAGTTTAACAGTTGTGTACGTCCCGGCGTCGCGGAAGTTTTAGCTAGCTTTTTTTGAAAAAAGGCATTAATGAGGCTTGATTTGCCGACATTACTCCGCCCCATAAAACAGACTTCCTTAACACTGTCTTGGGGACAACTCTCTAAGTCACTTGCACTTTTTAAAAAACGTGCCTCCATTTATTAATTACTTCTGGTTTTTAATGGAGCGCATAATTTCCTTGACTTGTGTTTCAGAAAGCTTACGACCCATTTTGGCATACATCGCCTTAATTTGGTTTTCGGTGATTGGTGGGTTATCACGAATCTGTTTTTTAAAGATTTTGATAGAAATAAAATAGCCAATAATGACACCGACTAACAGGGAAAGGGGAATTCCCAAACCTAAGGCAAGCGCAAGATCATTCATATCTATATCTTAATCTTAATCCTCTCTAGAATCTTATAAAATCAGAATAGCGTAATTAATGAACAAGCTAATTAGCATTCTGGTTCCTTGTTACCAATCCCAGCCCTTTCTCGATCGCTTTTTCAAATCACTTTTAAAGCAAGACTGGAACGGTGTTAAAGTAATCTTCTTTAATGATAACAAGCCAGATCCAACATACGAGATCCTAAAACAATTTCAACAAGCACATCCACAACTAGCGATTGAAGTACATTGTGGTGAGAAAAATGTCGGTGTAGGCGGTTCACGTGATCAACTAATAAATTATGTAGACACACCCTACTTTTACTTTGTTGATCCTGATGATGAATTTAGTGATCCCAACTGTTTTAAGGCAATAGTGGAAACAATTCAAGGAGAAAACTTTGATATTGCAGTGTTAAACAGTATTGTTTACCTCCAAATGTTGAAGAATGACTTTTTAATTAAACACATTCCGCTCAAAAACATCTTTCAGGGCAAGGTAAAACTCAATCCTGATAACACCGTAAATCATTTGCACTATATCCAGAATAACGACCAGTACATTTGGAACATTGTCATCAACACCGCCTTTTTCAAAGCATTGGATTTGCAGTTTGTTAACCGCTTTATTGAAGACATTGCGGTGTGGTTTCCGATTATGTTTAAAGCCCAAAAAGTGCTGTGAATTGATGTAAATGGGGTTAACTATTACTTGCGACCGAACAGTGCTTCCACCCAGAAAAACAGCATTAAATTACTCTCCTTTATTGAGGCTTACGAACGGTTGTATTTCCACTTAAAGAAGGTGGGTAAGCTAGCTGATTTTATTGACCCTAACAATAAGATTGAAAGTCGTTTTTGACGGCGTCAAGCCTTCATTTGGTTTAGTTTTATTAATGTTTCATGGATGAAAGCGGAATTTGAACAGACCAAAAGTGTGTTGCAAAAACTGTTTGACTTCATGGAAGCCAACGGCATCTACGACAGGGTATTTACTAATAAGCACCACGGTATTTACTTATTGTGAGTTAACCGCTTAAAACACTTTAAGAAGTTAGTGCAAGCACAACCACATCTATAAGTTTTGTGTTCTAACAAGCTAAAGCTTCGAGTAGCTTTTTAAAAAGTTGTTCTCTTTCTAGAGTTTGTTTTCCATCTTGGGCTTGTACAACCATTTCCTTTTGATTAGCGTGAATCTTTATGTAAAGTGTGCGTTTATTATCTTCATTGGCTTCGTTAAAAGCTACAAATGAAATTAGCTCACTTTGATTGAGTGAATTCATTGCGTGATCAATAAAAGGTAGTTTGATAATTTTTTTATTTTTCATAATTAATATTTGTTTTTTGGAGATTGAAAAAATAGCTACCACTTGCAACTCGGTTGCCAACAGGGATCAGACGTTTTGCTGATCAATTAAAAAAATAAAAGATTTGCATTTTGCTCCTCCACTAAATAAATTGAACTGAGACTTTATATGTGACAACAAAATTACCAAAAATTTTTTTGAATATTTTTTAGATAAATAAAAATACCAACAACTGACATATAAAAATCAACTGCTGGTACTTTTATTTTGGGTTGAATCTACTTAGACTCCAAACGATCCAAACGTTTGTTAATTACTTGAAGAGTTTCAAGAATTAACTTAAGCGTTTCCCCTTGGGTTTGAATTTGTTCGCCCTGCTTTTCAACAGTTTCAGTGAGCTTATTTATTTGTTCACCCTGAAGAATTGAAACTTTGATTAGCTGACTAATTTGTTCACCTTGAGAAACCAAAGTGTTCTCAATTTTTATCAGCCGCTGATTGTTGGAATTTTTGAAATCATTAAACTGCTTTTGGGTAACGTAACCACTTTTTCGTTTTTCCATATGGTTTAAATTGCCATAAAAGCCCATGTCATAGCTGCCATCAGGTAGATCTTCGATTCAGGCTTTTGATGTTTTAATCTTATCTTTTTTCAAATTTCTTTTAAACAAAGTAATTGAAATTTCACGTTTAATGATCAAATCCTCCTTTCTTTACAAGATTTAAAAAACTACAACGTGCAACAATGTTGCCAGAGGATATCAACTGCTTGGTTGATCCAGTAATTTTCTTGAAATACTTCATAACGATCATTTCACTAAATAAATTGAGTAGTTGATTTGGAAGTAACAAGTAGAATTTCTGAAATTTTTGAAGAGTCAAAAAATCTATTCATGAAATTAATTGAATGAAATTCAGGTAACAAGGCCTTTTCAAACTAAAAAAGCGCTTTTGCCGCCCTACTAATTAATCAATTGCGTAAATCAAATTGAAACGCCAAATTTATTTAACTCACCTCCCCCAAATCTTTCTTAAACTCACTTAACTTCCCCGCAGCAGCCAAATAATCCTTGTACTCGGATTGGATGTCGGTTAAGGCCTTTTTAAAGGTGGGGTTATCAGTGAAGGGTTTGGCCAAATCCGCACTTGATTTCAACAGCGCTTTGGCTAACTTAACCACTTCCGATTCTTGGGTATCAGTCGATTGCTGTCTGCTTTGCTGGGTAGTTTGAACGCTAACAGAAGCTGCTCGGCTACTCCCCTGCACACTGCCCTTAACTTGGGTCCCCTGACCCGAACCCATCATCGCCACCAAAGCACCGTACAACTTCGCGGTTAACCCCGTTTGTGCTCACGTTTGGAACAGGCTGCCAGATTCCAGTAAGTTCAAACTAAACTCGACGCCGTTTTCCTTAAAGTTATTACTCTTAGCTTGTAAATAGCGGTACAGCAAGATGTCGGCTTGTTTGGTGAAATCGCGTTTGTTGTTCGCCAAAAAATAATCACCACCGTCAATTGCCAGGGCGTATGTACCATCGTCACCACGGGCGTAAACAGGGGTTTGGAGTGTGGAGGTGGTTTGTTGAACTTGTTGCTGGGTGTGGGTAAGGGCGGCACTCCTTGGGAGCGGAATTAAAACAGAGTTGCCCCCGGAGCTACCACCAACATTGGTACCACTACTAGAACCTGCCCCAGTGGAAGCTGCCGCCATTGCTTGACTACTCGTCAACCGCACCGCGTCTTGGAGGTAGTGGTGTTTATCATTGGAATTATTACTACCCCCATTAGTTTGCCCGCCGCTCAGTTTGCGCTGGGTTGTGTAAAACGCATCGGTTTGATTATTCTGCTTCACCTTGACAAACGCCGGGTCGACCTTAAACCCGGGGGTTTGGTCGGTCATGGCAATTTTCGCACGCGAAACTATGGATCTAATCGCCACCGCATCCTTCCCCTCCATAAAAAAACTATCCAGCGGATTTTGGTTGGACTTATTCGAATCTACCTTGACCTCGGGCAACGAACTCGTCTTACTCCCACCCACCAGGTACTCGTACTGGTCAATAATGGCCGTAGCTAACCCCACGCCCTTAAAGGCCCCGCGCAGGTTGGACTTGGTCACCAAGAGCTTATTGGCAGCGCCATTGCTGTTTCACTTGGTTTGCCAGTCAAACAACCCGGTGCGGGAGGATGTATTGGATTGTTGGGTTTGACCATTCTTCTGCTTCGGAAACACCGCCTTAATGAAATCCCCGAAGTTTTGGGCACTGTTGGGGGTGGGGTCGAAGGTGGTTTTACCTTGGCTTTGTCCATTATTGGGCTCGCGAAACGCGGGGAAGGCATAGCCCAACTGTAAGGCACTACCAATCGCGGCTTGGTCGTATAAGCTGTCGAGCCCGTCCTTGGGTGCTTGGTACGCGAAGAACGCTTGGGTGAGGGGAAACAGATAGAGCAAAAGCTGTAAGCCACTTTATAATTAAGTAAATTATAGCAACGCTGTCAAAGCGTACTTTTACCATTGATTATTTCCTTAAAAAGACTTGTTTAAATGACCAAAACTAAGTTTAATCCGTACCAATTTCGCAAACAATTTAAGTGGTTTAAAAACAACCCCCAATGGGTTAACTTTGACAACGCTGCTACCTCAATTGCGCTAGACACCGTTAGTCAAGCTTGCAAGGAATACTATGAGCTTTTTAGTGTCAACCCCCACAATAAAACCCCTGATCTCAACAACCAGATTATTGCCATTATTGCTGAAACCCGTCAACTAGTAGCGGACTGGTTTAATGTCACTGCACTTGAAATTATCTTTACAAGTTCAGCCACGGAGTCCATTAATTTGTTTGCCCATGGCTTAAAGCCCTGGATTAAACCCGGTGATGAAATTGTCTTAAAGGGTGATGAACACTCGGCTAACGTTTTGCCCTGAGTTGCACTAGCTAAACAAACAAAGGCCAGACTAGTATGGGTCGAAAAGCAAGCTAACCAATCCTTAGAAGACACTTTTAAGTCATTGATTAACCCGAAAACTAAAGTAGTAGCCATTACCGCTACTTCCAATTTGTTTGGCAATTCAATTGACTTTGCTCAAATTGCCGATTACCTCAAACAAGTCAATCCTAAAGCTTTTGTAGCCGTGGATGCAGTGCAAACGGTGCAGCACAAGCAAATTGACATTGCCAAAACCCAGATCGACTTCCTAGCCTTTTCTACTCATAAGTTTTACGGTCCCACTGGACTGGGGGTAGCTTACATTAAAAAAAGCTTACAACCCCAATTACAACCCCTCAAACTCGGGGGTGATATCTTTACCCAGATTGACCCGGACAATACAATTCACTTTAAAAGTACACCCCTAAAGTTTGAAGCCGGTACCCCCAACATTATGGCCATCTATGCCTTAAACAAGCTGTTGCGCTTCTTTAAACAAAAGTTTAATTTTGCCCAAATGATGGCTTATGGTCACCAACTTAAACAACAAGCTTACGAACTGTTAAACAGTAATCCGCAAATTGTGCTGGCCAACCACGACCAGGATGTCCCGATTTTTTCCTTTAAACACCGCCAATTGGCTACCATTGATCTAGCCACTTTCCTAAACATCAATAAGATCATGGTACGCCAGGGCTCCATTTGTGTGGGACGTTACCGCAATAAGGATTACTTTGTGAGAGTTTCGTTAATGCACTATAACACAGTGAAGGAGTTGCAGTATTTAGCTAAACTCCTAGCAACTGATACCAAGACAATTATTAAAAACGTAATTAAGTAACTATGGATCGCAAAATAAGGGAGCAAATTATTGCCATTTACAGCAACTTACAACACAAACAGGACTTAACTCAGTTTGCCCATTGTTTAACCACTAAGGCTGATGATAACTGTGAGGACTTCTTTAACTTAGGCTTACAGTTTCAAAACAACCAACTAACAGCACTTGGTTTTAACGGGGAGGGTTGTATTATTTCGACAATTGCTAGTGAACTGACTTTAAGTGCGTTGGAAGGCAAAACAATCAAAGAAGCCATTGTCTTGTTAGAACAGTTTATGGAAGCTGTACAAACTGGTAGCTTAAGCACGCCACTACCCCAAGCTTTACAACTGTTGTGGGACTTTCAGATTGACCAGAAGCGTTTAAACTGCTTACTCCTAACACCACAGAATTTGCTACAGTGGCTTAAAGATTTTTCGCACTAAGCGCTAGCGTTTGCCCTAACGCATCATACAAGCGTTTGTCACCCACCAGGATTTGCTGGTGACTACGTTTGTTTTGCACCTGGTTAGCGCGCACTAAGTCTAAAAAGGCTAACTCTTGCGTACCAGCGTCCAAGGCTTGTTCTACTAGTAAAGCCATAAAGATTTCCTTTTTGAGGCCTAAAGCACTGTTCTTATCAGTTTTTCAACTAGCAGAAATTTTGTTAACGTCCTCGTTGGTAATCTGAATGACATAGCTCAAAGTCTTGACACTCGAGTCCTTGGTGAGTGGTACGGCTTTGGCTTTCTTATAGTCTTCGTTACCTTGGGAACTACTACTTGAATCAGACATCTTCACCTTGGTTTGGCCAACATAACCTTCGAACTTGTTAAAGTAAACATCATCCAGTTTGTCCACTACATCAACCAAGAACTTCTTCTTGTTGTCAAAGCTAATCAACGGTTCCCCACTGGAGTTAAAACGTCCTAAGTTACTGGTATCGACATTAACTGCACGGTGCAACACTTCACCAAAGGCATTGAGCTCGGCATCGGAATTAGTGTTTTGGATTAAGCGTTTCAGGGTTGCTTTATCCTTGTACTGGCTCAAGGCCCCGGATTTGTCACTACTTGTTACTAACTGACCAAAGTTGTTAAAGATTTGTTCCGTGAGTCCGGCACTAGCAGTCGACACACTTTGGGGCGAGTTAATCGTTAAACCGTTAAAGCCAAAGAAGTGTTTTTCGTTAGTGGTACCACCAGTAGACCTCTTTTGACTTTTCGTCTTATCATCAAACGGTTCCGCGGTAAAGTTGTAAGTGCTTTCGCTTTCCAGTAACGAATCGGGTTTAATTGGCGTACTCTTATCGTTAAATCAGTTGGTTTCCGAACCCTGGACATAATCGGGGTTTTGGACAAACTTATTGAAGGGGTTGTCGCTTTGAGTATTTGTTGCCTGAGCTGTCTTTAATACTGCTACTTGCGCTTTTGCTGCGGCTGCTGATGCCGTAGCCGTATCATCATAGCTCACCGTTCACGACACAAAGGCACGGGTACCCACGGTAATCGCTTGTTGGAGCAAGCGCTTATAGTTCTTCGCCCCATCCTTAACTAGTCACATTAAGGTGGACAAGAAGCGCAGGTACTTGTACTGCCCATCACTACTAGATGCTTCCAGTTCACTTTCAAACGCTTTTTTACCGAGTTCCAAGAGGGTTTGATCACCAGATTTTTGCATGCCACTTGAAGCTACAGCATTTGCAACTGCTGCGCTACTTGAAAGTGAAACCATTCCTGATTGAGCTGCTTGTGCTTTTACACTACCCAACTCCTTGTAATAACCAAACTTTTCCTTGGAAGTGAGTCGTTGTCAGGAACGTAAGTACAAGGTTTGTTTAACGAGGTCGTTAAAGTCCTTGTACTCATTGCCTTTAAGTTCTTTCTTTTCAGAATCGTAATTGTTCTTAAAGTCGTTGGTATTAGTAAAGAACCACTCCTTAAAGGAATTGAGAATCTTGGTTCACTTAATCCCAACATCAGACAATAAGGCCAAGATCATGTTGGTCTGGGGCTTATTGTCCTTACTCTTGAGCGAAACAATTTCGGAGAACTGACTACCAATTTGGGGTGTTGCACCTCATTTGGTGATTAAACTAGATGCTTTAGTTTCTACATCACTAGTTAACTGCTTTAACTTTTTGTCGTTGTTTTCCCGGGTCGGTTTGATGTCCGGTTGTTTAACGGTGTTTGTGCTTTTTTGACTATCTGTTGTCTTTAGTGTTACTAAAGCGGCAGATCCTGATTCAGATGCTTTTACTTTTCCATCTGCTGCACTATCTTCCGGAATAAAGAACTTCTCTAAAGCGGGGTAACTACCATCACTAGCGCGTTGGTAAGGGAGCGGTTGGCCCAAGCCAATCGCACTCTTTTGGGCTTGTTTTTCTAGTTGAATGTACTTATCAGCGCGGTCAATAATAGCTTGCTGTAAGCCACTCACCTCCCCCAAATCTTTCTTAAACTCACTTAACTTCCCCGCAGCAGCCAAATAATCCTTGTACTCGGATTGGATGTCGGTTAAGGCCTTTTTAAAGGTGGGGTTATCAGTGAAGGGTTTGGCCAAATCCGCACTTGATTTCAGCAGGGACTTGGCTAACTTAACCACTTCCGATTCTTGGGTATCAGTCGATTGCTGTCTGTTTTGCTGGGTAGTTTGAACGCTAACAGAAGCTGCTCGGCTACTCCCCTGCACACTGCCCTTAACTTGGGTCCCCTGACCCGAACCCATCATCGCCACCAAAGCACCGTACAACTTCGCGGTTAACCCCGTTTGTGCTCACGTTTGGAACAGGCTGCCAGATTCCAGTAAGTTCAAACTAAACTCGACGCCGTTTTCCTTAAAGTTATTACTCTTAGCTTGTAAATAGCGGTACAGCAAGATGTCGGCTTGTTTGGTGAAATCGCGTTTGTTGTTCGCCAAAAAATAATCACCACCGTCAATTGCCAGGGCGTATGTACCATCGTCACCACGGGCGTAAACAGGGGTTTGGAGTGTGGAGGTGGTTTGTTGAACTTGTTGCTGGGTGTGGGTAAGGGCGGCACTCCTTGGGAGCGGAATTAAAACAGAGTTGCCCCCGGAGCTACCACCAACATTGGTACCACTACTAGAATCTGCCCCAGTAGAAGCTGCCGCCATTGCTTGACTACTCGTCAACCGCACCGCGTCTTGGAGGTAGTGGTGTTTACCCTGGGAGTTATTATCCCCACTTTGCCCGCCGCTCAGTTTGCGCTGGTTTTGGTAAAAGGTATCGTTCTGCTGACTCTGCTTCACCTTGACAAACGCCGGGTTGACCTTAAACCCGGGGGTTTGGTCGGTCATGGCAATTTTCGCACGCGAAACTATGGATCTAATCGCCACCGCATCCTTCCCCTCCATAAAAAAACTATCCAGCGGATTTTGGTTGGACTTATTCGAATCTACCTTGACCTCGGGCAACGAACTCGTTTTTTTTCCACCCACCAGGTACTCGTACTGGTCAATAATGGCCGTAGCTAACCCCACGCCCTTAAAGGCCCCGCGCAGGTTGGACTTGGTCACCAAGAGCTTATTGGCAGCGCCATTGGAGTTTCACTTGGTTTGCCAGTCAAACAACCCGGTGCGGGAGGATGTATTGGATTGTTGGGTTTGACCATTCTTCTGCTCCGGAAACACCGCCTTAATGAAATCCCCGAAGTTTTGGGCACTGTTGGGGGTGGGGTCGAAGGTGGTTTTACCTTGGCTTTGTCCATTATTGGGCTCGCGAAACGCGGGGAAGGCATAGCCCAACTGTAAGGCACTACCAATCGCGGCTTGGTCGTATAAGCTGTCGAGCCCGTCCTTGGGTGCTTGGTACGCGAAGAAGGCTTGGGTGAGGGGGAGGGGGTTTTCCTCCACTACTCACTGGGCAAATAGATAAGCCTGGAGGTTAGCAAAGAACTCCCCGTTAGGATCATTTGGTTTGGAATTAACAAACTTGATTTTGTTGCTAGTACTGTCGAGGTTATCTTTGGTAGTGGGTTTAAACTGATCACTACCATCAGTCAACTGCACATTAGCAACAATGTTGCTAAAGATAAGTGATTGGAAGTCGGCTATTAAACGGTCGTATAACTTTAAGGCCTTTCAGGAACTTTCACTCCCACCCCACTGGTCGTATTCCTTTTGGAGCTCTATTGGTCAATTAATCGGTTCGGCCTGTTGTTTTTGGTCCAACAGGTTATCATGTTCATCCTCCACGTTTTCACTAAAGATAGTTAAACGGGTAGCAATGTTGCGATCCTTATTGTTTTGGTACCAACTCTTTAACGCTTCACCAAAGTTAACCCTTAAAATGGTTTCCGTACCATTGTCCTCAGCTAAAGCGGCTTTTAGTACAGCTTCAGGTTTAATGATGTTGTTTTCGGTAAACCCAATCAACTGCTTGGAGCGGTTTTGACGGGGAAACAGATTGGAGGTATTGAGTGCAGAACACGCCGCTAGCACCACCCCTAGGCCAATTCCGGTAAATGTAAACGCAACTAAAGGTCATCGCTTTAACTTATAACCCATAAATTGTGTGTCCGTTAAAGAATAATAGTTTGCTAATTATCTCATGAATGGCACTAATTTAGCCATTGTTTATCCAGCTAATAAAATAGGTGAAGGTGAAAAACTTCAAACCGCTTTTACAATTTTGTGAGTTCTTTTGCCCAATTAAAACATTGGGCAGACCAGCATTAGATTGCTATGGTATATTTAAGCGGGACTTGGGAACCGTTAATACTATCTCATTTTCCTCAAGATACAACTGTTTCATTTTTTCGTAACTTTTATATTTTGTAAACGTTCCTCTTATCATTCATACATGTCTTTATAATTCCCAAGTTCTAAAGAGTCCCTTCGGGGACTTTCTTAATGCTAAAAAAGCAGTTTGATGCTACTTATAGTCGCTATAAGCCATCTAAACTGCTTTCTTAGTTTTGATAGTTATTCCTAATGTTGTGTCTATATATTATATATAGTCCTTATTAATAACTATTCTTATTAATAACTATTTTTATATTTACCGGCGCTGTGGGGGTTGGCTAGCTCGCTGGGGGTGGCGGGCTGTGGATTTGCTTAATCGTGAGCTGCTTAATTTGTTCAAACAACTCTGGTTTTTCCGCCAACACGCTAAAGAGCTTCTCTTTGCCAATCGCAATGTTTTGGCTCTTAAACGAATATGCATTGTCACTGCGCACCACCACATTGTGTTTAAGCGCTAACTCAATCACTTCGTGTTCATACACAATACCGCGGTTAAACATAATTTCCAAAAAGGCAACACCAAACGGGCGCGCTACCTTATTCTTGGACACGGTTAACTTACTCTTAATGCCGACATAGTTACCAAAGCGGTCTTTGAGCAGTTCACTGCGTTTGGCCTCCATCCGCATCGAAGCGTAGAACTTCAACGCCCGACCCCCAGTAGTTACTTCCCCATTACCAAACATGACTCCGGGTTTTTCCCGGAGTTGGTTAATAAACAGTAAGCAGGTGTCAGATTCAGGTAAGAGTGACTGCACACGGCGTAGTCCCTTAGACATCATCCGCGCATGGAGTCCTATTGTCTGGTCATCCATGTTGCCCTCCAGTTCTTGCTTAGGAATCAAAGCCGCCACGGAGTCCACCACAATTAAGGCCACTTTGTTGGTCTTAATCAGTGACTCCATTAGGGCAAAGGCGTTTTCCCCATGCTTGGGATGGGCTACTAGTAATTTACCCAAGTCAATCCCAATTGCCTTGGCGTAACTTAAATCTAGTGCACCCTCAGCATCAATGTAACAGGCCACTTTGCCCGCTTTTTGAAAGCTAGCGACTGCATGTAACGCGACCGTGGTTTTACCCGAGGACTCGTTACCATAAAGTTCTACAATGCGCCCTAGTGGCAAACCACCAGTGCCGAGCGCTTCATCTAAATGAAGGCTTCCAGTTGAGACAGTTTTAATTTCACTGTTTTTCTTTGCATCTAAAAAATCAAAGTCTGCCAAGTTATTGTTTTGAATAAAACTGTTGTCTTGGCTAATTTTCTTATTAATCATTTCTTTTTGTACCATTGTCGTTTACATCATCTAAATTGATTCAAAAAGGGAAAAACGCCATTTTTTTAGATAATTAAGGCAAATTTTTATGAAATTAACACTTTGGCTGGTGTTGGGCGCTGTCGGAGTCGGTGCAGTTGGTACGGGTGTGGGCTTTGGTACCAAGTACTTTTTAGACCACAAAGCCAAAAGTAACGTGGACGTTGCGTTTAAACAACGTTCACTACGCAAACAGGTCAATGTGGGCTTTTGGAATGCACTCAACTTCTTGGGTAGTGAAAGAAGTTTCGGTAACCAAAACAGTAAGGTTAAAACCAAGGGGATTGCTGAAATTATTAACCTCCTTAAGTACGACATTGTCGGTTTAGCGGAAATTCAACCATCCACTGAAAGTACTGCTGAACGGTTTGTCGACCAACTCAACGAGTACGCTGATCCTTATACCTCCTGGTCTTATGATCTATCTCCCGTAACTACCAGTAAGACTGCTAGTGAAGGCCAGAAGGAACGGATCTTAATTGTTTACAACAGCCAGAGTGTCAAAATGGAGGGACAAGGTTGATTTTATGACAATCCCGAAATGGACATTAGTAACTATGTCAGTGATGGGAGTTCTTCCAAAACAAGAACACCTAAGAAAACTAAAACTAGTAAAAAGAAGCCTATTAAAAAGAAAAGTTCAAAAAGCAAATCTTCCAAAGGTAGTAAGAAACAAAAAACTACCAATGAAAGTGAAAGTGAAACTTTAGAACTCAAACTGGAACAACGCCGTGTAACAACACGCTCACAATCAAAGCAACAAAAGGGGCAAGAACAAGCAACTGATCAAACTGATAGTGAGGGTGTGACTACTGAAGAAGGAGCAGATAACACTGACACTGAATTAGTTGAAACCACAGCGGAAACAACCGAACAAGAAGCTACCACGAAAAGTACAAAAGACACTAAGGAAACTAAAGATAGAACCAAAGTGGACTGATCGCGTCCCCCTTACAGTGCTGAATTTAGTACCCAAAAGGGCAAAGTAGTAGTGGCTTTTGGTCATTTTGACTCTCCGGGGGCAAACACTAAGCGGGGTGAAAAAATCGCTAAGATGGGCAAGGGTCAAGGTGCCCATGAATACTTTGAAGCGCAAACTACCTTCAAGGCAATGGATTCCATTAAGCAGCACTTTAACAACGAAAACATCCTGTTTATGGCTGACACTAACATTAAATTCGGTAATCAAGCCGCTGCTTTTGGTGAAAGCAAAGATTACACCTTTTTAACTGAAGATAATGAAGCGTGAAAATCTTCATTGGGTACAAAGAGTGGTTATGCCAACCCTTATGACAAGATTATTACCTCTAACAGCTTTAAGAATAAGGTCTTGGATCAAGCTGAAACCTACTGAAATTACTTTGACCGTTACTTAAAAACTAAGGGTCACAATAGTTATCTGTTTGATCTAGCTAGTTGGGGCACTAAACCTAGAACTATTTCTGACCACGCCCCTGTAGGAGTGTTGTTTTAAGTTAATTAACAGCGCAAAATATAAACATGGCGGATAAATGTGTGACCCTTTTTGGGTTCCGCCATTCATAAGGGCGCTCGGGAAACCGGGAGTTTTTTATTGCTAAAGAATACAAGTAAACGGCTTTTTAGGCAAAAGCTATTTAACTCTCTTTAAAGCCAAGTCCCACTGCTGTTCGTAAAACTGCTTGGCTTGTTTTAACTGCGCCACATTTTGCACGGTACTTTCAGCAGGATCATTTTTCGATCACATTTCAATTAGAATTGGTACTGTTGGTACATACTGGTGAATGGCTTTTAAAGCTGCTTCAAATTGGGCTGTACCTGTGCCAAACGGTACTTCCTTAAACTGCTTGGGTAAAGTATCTTTAAAGTGAAAGCCCACTATCTTGTCTTGACCTAATGCGATTTCATCAAAGACTGCCGTACTAAATTGCGATAGGTTACCAATATCCGGATAGAGCAAAATGTTAGCCTTACCAATCCGCTTAATCAAGTTTAAACAACGCACAATCGTGCCAGCAAACGGCGTGTCCATCACCTCAAAGGCAATGGTAACAGCGTACTTTTGCGCTAACTCTGCCACTTTCCGCATGTTAGTAATGAAATTGCGTTCTGTTTCTGTGTCATGTGGCTCATAATACACATCATAAGCCGCTAACTGGATAATACGAATCCCTAGTTTGCGTGCTAACACACAGGCCTTTTCAAAAATGGTTAAGCCCTGTTGGACAGTAGTTTTATTCTTGGAACCCAAAGGAAAGCGCCGGTGGGCACTTAAACACATTGAGTTTAAACAAAAGGTTTGTGCTTGTAACTCATTGTGGAGTTGGTTAATTTCGGTATCACTCCAATCCAAGCGTTGTAACCTAGCATCCGATTCATCAATGCTCAACTCAATAAAGTCAAAGCTAGCTTGCTTAGCAATAGCAATTTTGTCCTTTCAGGCAAACTTATTGTTAATTGCCTTTTCATAAACACCCAGGAGGTGTTTATTTTCAGTACTTGTGAAACTCATCTTTAAATTGTTGTACGGTTTGAAGTGGATCACTAGCATCCCTAATGGTACGCCCAGCAATAAAGATATAAATGGGAATATCCTTAAATAACTGGATGTCGTTGAGGGTAATGCCGCCGGTAACGGTGACCTTAAAGCCCAAATCCGCTAAGCGTTTCACTGCTTGCAAATCCTTATCTGATCAGTTGACTCCCGCTGCTTGGGCATCACGGGAACGGTGTCACACTACCTGCTGAACTCCCACTTGTTTTCACTGCGTTACCTGCTCCCAAGTGAAGTTACTGGTCATTTCCACCTGGGTTTCTTTTATGGGCGTGTAACTGTTACCCACAGTTACCACATCCTTTACGGTGGGCAATTCCGCTGCACAAATGACCGTGGTAAAGTGTGCCCCAGCATCAAAGAACATTTGGCTAAACACCTTCCCCGCATCAGCAATCTTGCCATCAGCCACTATTAACTTATGGGGATAGCGTTTGGCAATTTCTTTAACAGCATTAACGCCCTCCGCTGTTAGTAAGATAGTGCCCACTTCAATAACATCAACAGCATCCCCGACTTTAGCCAGGTCATTTAAAGCACTCGCTAAACTGAGGTTATCAAGCGCAATTTGGATTAAAGGTAAAGCCATATTAAGTTAAATATTTGTGTAAGTTAACCCGATCAATCACAGCAAACAAGCTGGTCTCATCACAACAGTCCAACAAGTCCTTAACCACTTGAGTATCTTCTACTAGGGCCGCAATTTGTGGTAAAGCTTCTGTCGTATGAATGGCAGCGCTGGTAGCCGCTAATCCAATTAACAAGCGCACCGGGCGGGGATCGTTGTCAAAGTAAACTGGTTCCTTTAGGGTCACTAAAGAGAAGCAGTTTTGCTGTACCGTACCGTTATTCTGGGCATGAGGCATGGCGACATTTTCCGCAATAATGAAATACGGACCGTACTGAGCTACACTCTTTAGAATGTCATCAAAGTAAGCTGGTTGGACTAACTTGGCAGCAATTAAAGGATGGATGCAGAGCTGTATCGCTTGTTTTCAGTCCTGCGCGGTTTGGTGCAACCGCACGGTGTGGTGTTGCTTTAAGACGGCCTTAAAATCGAGCTTTGTCATCTTAAAGCACCTTACTTAACGCTTGCTTAATTTCGTTTTCATCCATCAGGTTTAACACCCCAACAATCTTGGCCTTAGCATTCGGGTTAAACTCACTCGTTAAGTGAATCGAGGAAATAATAATGTCAGCGCTGTGCTCCATCCCCTTGGTTTGTCCCATTGACAAAGCTTCCACTTTGGCGGTGTAGCCCAACTCCTTCATAATCTTTTCAACCTTAATCTTGATTAGCATGCTCGTACCCATGCCGTTACCACAAGCGGCAATAATGTGTAAGTTCTTATTTTCCATGGTTAGTTATTTAAATCTAATTGTGGGTTAATTTTGAGTAATTGTTGTAATTTAGTTACTTTAGTTTGCTTACCATTGTCAATAATTTGTGCCAGCAAGATTAAACCAACGGTAGCCAAAATTACTAAGATCCAACCACCAATTACATTGTAAGCCCCAATTCAAACTAACAACCCAAAGTAAAGATTCCAGTCAGCCATTCCAATAAAACCGTTGTCAACGGGTGATGCACTTTTACTGATCCACTCAATTGCTTGTGCTAGCGTACTGTTAACAGCTTTTAAACTGTTAAAGCTATCGGATTTAACAAAGCTTTGCGCTTCGGTTATCCGGTTAACACCAGTAGCATTTACAACCTTTTCTACGGCTGTTTTTAATGCCGAACCGTTGTTATTGTCCAAACTATTACCCATTAACGCCTTTAAATCAAAGCTTTGTTTAGCATCTAACAGTGCCGAAGCCTGGTAAGCGTTTGATACGGTGCGCAGCGCAAATGAAATCACAATAATTTCTAAAAAGCCAATAATACCCGGGAGCAGTAAGGCCGCCTTCCAACCACCCTCGGCATTAGCATACACGCCAAAGGCACCACTGTTAAAGAAGAGTGTAATAAACAAAGGAATCGCCACAAAGCTAAACTGGTTACCCGGAATATAGCTAATGCCAGCCATAATGGCAACAGCTAAAAACTGACCAATGACCCCACTTAAAAACCCAAAGGTCACGGAGTTAATTGAAAACCCATAAACAGCTGCAATATCAACCGCTACCACGGCCCCTGGTACTACCTTTTCACTAATACCTTGAAACGCCTGTTGTAACTCAGTGATAAACATCCTTACCCCAGTCATAATGGCAATTAAGCTGGCCACCATTTTCAAAGCACCCCCAATAATGTTGAGCGTAAAGTTAGCTCCGCCAAAGGTAGTGTTTCAAATCTCTAAACCATTAATGGCACCAAATTTGTCCCAGTTAATTAACAGTGGTTTATCACCCTTGTAACTAGCCAAAATCACAATAAACAACACCACAAACAAACTAAGGATAATGATCGTTTGGGTGAAGATATTATCTTCAAAGATCTTTAGGGCCTTAGGCAGTTTTTTGTGCTCGGCACTATCGTTTTTGTCACCAAAGTACTTGCCCAATTTAGCAACCGTCATAATCCCTAACATCTGCTGGTGACCAATCGAAAAACCAGCATTATTGGTTACCTTATTGGTCACTAAATAGGTACCCGTAGAGGCCGTAGACCAGTAAACTCCCAAAAAGATACCACTAATAATCACTAGACCGGCTTGGGCACCGTAAGCAATGCCCGTACCCAACAACGGAATTTGGCGGAACAAGACAATATAGAACAGCGTAGTAACTACCGCTGCTTGTTGCAACATAACGTGTCCAGTAACCATAATCGAATTGGTGTTAGTTCAACGCTTTAACCCCACAAAGATAATGTTGACAATAAAGCCCACTAACAGCGAAAAGGCAATCAAGGACACATAGTCATTGTTCAAGAAACTGTTCGTAAAGAAATCGTTCGCTGATGCTAAGGAAAAGTAAGGATCCAATAGTACCACACCAGTACCCCCTAAACCCTTAATCGTGTCAAAAACGGGACGGGCGGTGGACACCAACACGCCACTACCAATGCTTAACAGGATAAAGCCAATTACGGTCTTTAGTGCCCCCACCACCGATTGCACCCCACCACGACCTAAAGCAAGGTAACCAATTAGGGTAATAACACCTAACAATAGCGCTGGTTGACGCAAAAAGTTATCGAGGTATACCCGCGTAATTAAAAAGATAAAGGCATCACCAAAGGCTTGGGCCGAAAACGGGGTTACTCCCTTACTAACTAAGTTCGGTAATCCTGCACGCAGTAATACTGTCAAGAGAATTACCACCAGGTTGATCAAAGCAAAAACGGATCAACCGATAATTAACTTACGTTTTTGTTGGAGCTTAATTTTGTTGAGCATTACTCTATTTTTAGTTGTTTTTTAAGTGCTAATACCCGTGGATGCACAGCCTTGGGATTAAACTGCCGCGGTGCATCAAAGGCCAGGATTTCAGCGAGATTTTCGACCAAGATGGTGTTAATGGCCGCATCGGAAACTCCCACTTCTTTAAGGAGCGGAATAAAGCGTTCAAACAGATATGCAAACCCAAAGCACTCCTTCCCTTTTTCTAAACCATAGTGTTTTGGTACAGTACCCGCCCCGCATCTAAGGCCAGGGTAATGTGTTTAACAAAGCCCAAGTCCACTAAGTATTTAATGTGTTTAGCCAATAAACAATCGGGGTAATACTTCACCCGATCGGGCCCATCAAAACACAGGGTCACACCCAGTTCCCGAATAATCTTGGCATAGTAATACTCATCGGGATTCTTGTTTAAGTGCGATAGTTAAATTTTCCGCGGATTAACGCCAAAATCAATCAGGTGTTGTGCTGCTTCATATGCCATGGTACCGAGTTGGGTGTGCACGAGTACGGGAGCACCAGTAGTAATCGAAGTAATCGCGACTGCTTCCAAGGCCTTTAATTCCAACCGGTCAATCGCAGCATAGCCCGTACCAGCTTTAATAATGCCTGCCTTAGCCTTGCCCCGTTTAACCACGGGACCGGAATAGTTATACAAATCCATACCCTCTTCTATTTCAGCGACCAACATCGGCACAATTTCATTAACTGGCACTTGTGCTAACCAAGAACTTCCCTTGTCATAGAAAGCAGCCTTGTGAAAACCAGTGGCCATAATAATGTTCAGCTTGCCCTTTAGTTGTTCTGCAATCGCTACCATCCGTTTGACATCACGGCCCACATTCGGTGGATCCATGGTGACAATGCTCCGACCACCATGGTGGACAAATTACAAACACTCTTTAATAGCAGCTTCATTCGATAACATCACAAAATCAGGGTGCTCTTTGGCTTCCGGACCCCAATTCTTAATTAAATGATCGTGACAGTCACAAATACCTAAATCCTTAGGGTCAATGTCCCCTAAAACTGTTCTTACAAATCTTTTCATTTCTAAATGGACGTTAAATGGTTTAAGTGTATTGAAATAACCCTAATAATGTGTAAGCTACTGGATAATTTGCCATTAACAGTTGTAAAGCTGGCAACTATTTTTAGAATCAGCCATGGATCAAAAAATGATAAATGACCTCAAAGAACAGGTTTTTCAAACGAATTTATTACTACCCAAATATGGCTTAGTCATCCACACTTGGGGTAATGTTTCGATGATTGCCCCGAACCGTCAGTTCTTTGTCATTAAACCAAGTGGTGTGAGCTATGACAAGATGCGTGCCCAAGATATGGTAGTGGTCGATTTGGACAACAATGTTCTAGATACCAACGGCCTCAAACCATCAAGTGATACCCCAACCCATGCGTTAATGTACAAACATTGTCCAGATATTAAAGCTATTGTGCACACCCACTCTACTTTCGCGACATCGTTTGCTCAAGCCGATAAGCCGATTCCTTGTCTTGGTACTACCCATGCTGATAACTTCTTTGGACCAATCCCGTGTACTAGGGCTTTAAGTGACAGTGAAATTAATGGAGCTTATGAACATAATACTGGTTTAGTCATTCTGGAACACCTCAAAAACAACCAGGTTGATGTCAATGCTTGTGCGGCCATCTTAGTCAAAGAACACGGTTCGTTTGTTTGGTCCAATAAGAATGGCAAGGATGCAGTGGACCGGGCGTTAACTTTAGAACAAGTGGCCCAAATGGCCTTATATACCCAAATGATCAATCCCCACATGAAAGAAGCAAACCCAGCGTTACAACAAAAGCACTATAACCGCAAACATGGTAAAGATGCTTACTATGGACAAGATACTAAGCAAGAAGATTAAAGTGAACTGGTTGGGCGGGGTGTTTTGGTTATTACCTAACTTACTCGATCTGTTTTCCGCTAGCAAACGTAAAGCGAGTGTGCGACCCTACCAAAGCTTATTGGAACTGGTTCAAGAAAACTTCTTGAACCGGTATGACCTCGTGCACTTTAGCTTTAATGGTGACCATGACTTCTTTCACTTTAATGATTTACAAGCCATTAGGAGCTTTAACTTTACGATAGAAGAGGAACAACTAGGAGCAATGCAACCAGATGAGGTTTTACTGTTTGAACCTGTGGATCGTGTAACAGTGGAATTAGATCAAAAGGGTTTGAGTTTAATCCACAGTGGCAAAGCTTTTTGTGCTAGTGCAAACTACTTTAAGCACTGGCTAAAACGCGTACCACAGCAAGACAAAGTGACCCTAGTGTGGCGAAAATCAGGTTTTGAATTAAAGCAGTAGAATTACTCTAATACTATATAGCACTTCTCCCCCACGCTTTCCCGTTTCTCCACCGGGTTCAACCTCGCGGGGTCGGTGCTCGACCAGGTGTTTGACTATATTCCCTGGATTGGGAATGGGTACAGGTATGGGAATAACCACCGGTGTGTGGATGATATAACCGCGCCTCAAACCAGCGCGGGGTCGTCCAGCGGAACTAGTACGAACACAAGTGGTTCGCGTTCCTTTCTCCCGACGTTTTCGAATGTCGGCGTTGGCCTCAAAGCGAATGTCCAGGGCACCCTCGGGGGCAGTCAGACGACGACTACGGGGAACAATATTCCCAAATGACCCACCCTCGACCAAGCCAACCTCCAGCTCTGAACGGGGGCGGGGTGAAGGAATGATAAGACTACAAGTGGTTCAACCGGCAATGCCAATGACACCAAGTTCACGAGTGCTACGGGCAGTGGGAGTGGGCAGGGCAGTTCTTCAGGTACAAATACTTCCGCGGGGAATCCTGATGGGCTTCAGGCTGATAAAGTTGATCAAAACGGTCAGGTGAAAACAAGCGTTCAAGAAGCCACTTCAGGGGACAACTTGACGAATTACACCAACCTCCCCCCCGCCAACCTCACCCCCACCGCTGATTGACCGAACGCGCTGTCATTCACCAACAAGAACAACGCGCAGCGCGCCCAGCTGTTCCTGCGCGGCCTGTTGGGCAGCATCCCGGTGTTGGTTAATAAGTCCGGCCAAGATGATAACAGTAAGTTTAAGGCGGAGGACCAAAAATGGTCCTACACCGACTTACAGTCGGACCAAACCAAACTGAACCTCCCCGCTTACGGCGAGGTGAATGGGTTGTTGAATCCGGCGTTGGTGGAAACCTATTTTGGGAACACGCGAGCGAGTGGTTCGGGGTCCAACACGACCAGTTCACCCGGTATCGGTTTTAAAATTCCCGAACAAAGTGGCACAAACACAACGTCGAAGGCTGTGCTGATCACCCCCGGGTTGGCTTGAACGCCGCAAGACGTTGGTAACATCGTTGTCAGTGGCACCAGCTTCAGCTTCCAGCTCGGCGGGTGGTTAGTTACGTTCACGGACTTTATCAAACCCCGCGCTGGTTACCTCGGGCTCCAGTTAACGGGTCTCGATGTAAGTGAAGCGACCCAAAGGGAGTTAATTTGGGCCAAGCGGCCCTGAGCGGCCTTTCGTGGCAGTTGGGTCAACCGGCTGGGCCGCGTGGAGAGTGTGTGGGATTTCAAGGGGGTGTGGGCGGATCAAGCTCAGTTGGCCGCGCAAGCAGCTACAAGTAGTACCACCACCACCGCAACAGGGGCTACCTTACCGGAGCACCCGAATGCCCTCGCGTACCAAATTAGCTATACCGACAAGGATTCGTACAAGGCTTCCACTCAAGGTTCGGGTCAAACCAATTCCCAAAACAATTCGCCCTACCTCCATTTTATTAAACCTAAGAAAGTCGAAAGCACGACCCAACTCGACCAGGGCTTAAAAAACCTGTTGGACCCCAACCAGGTTCGCACCAAGCTGCGCCAAAGCTTTGGTACAGACCATTCCACCCAGCCCCAGCCCCAATCGCTCAAAACAACGACACCGGTATTTGGGAGGAGTAGTGGTAACCTCAGTAGTGTGTTTAGTGGTGGGGGTGCTGGAGGGGGTTCTTCAGGCTCAGGTCAATCTGGCGTGGATCTCTCCCCCGTTGAACGGGTGAGTGGTCACTAACTATTAATAGGAACTTTTCTTATTAATAGGAACTTTTACTTTCAAAAAAGTTTTGAAGTTATTTTGGTGTCTTTACCAACTTTAAGCAATTTAGTGAACGAATACCCAAAAAGTATGAAATTAGTTAAATTAGCGAGGCTTCACCGCTTTTTAAAACAAGACCAATTGCAACTTGTTCCTTTGTTTAGTCCAAACTTTTTGCAAGTTTTGTTGGCGCTTCTCAATAAACTGGTCAATTTATTTAGTGAAAACACAACAATGAAAACAAATAAAAAATACGCCTTTGCGGCGTTGCTAATGCAAGCTCAAGCTAAGCTCCCAAATTCATTTAAATTCTTTCTTTCAAAGAAAGGAGGTATGGCTTAATGAAAGAGAAAATTCCGTTTTATAACGAAAAAGAATTCCATGACATGGTGAAAAAGACCAAAAAGGGCACTTTTTCAGGTTGATATATCATTGACAAAGATAACAACAGTGTGGAATTCTCTGGCAAATTCAACAGACAATTTAAACTTAACAAACCGGTTATACCAGTGAACACGGAATACGTAACGCGTAAAGAGTTTAACGAGTACAAAAACTCAAACGACCAACGACTTACAAAGATTGAAACCACTTTGGCCGCCCAAGGTGAACAAATCAACAAATTGACTCAAACTGTTGAAAAACAAGGCGAGCAAATCAATCAATTAGTTCAAGTTGTGCTTCTTCACGGTGAACAAATCAACAAATTAACTCAAACTGTTGAAAAACAGGGCGAGCAAATTAAAGAACTTCAAATCGAGCAAAAAGCTCAGGGCGAAACCTTAAAGCTAATCTTGCAAACGCTGCAAAAGATGAGCGATCGCTTGGACAAGATGGAAGTAAAAATGGACAAAATGGAAGTAAAAATGGACAAAATGGAAGTAAAAATGGACAAAATGGAAAAAAGAATAGACAAACTTGAGTCCAAATAACCAATGCTCAGAAATAAAAATACCAACAGTGAAACCTAAAGATGCGGCGCTGTTGGTATTTTTTTCAATTCACATAAATAAAAAAGCCATCTGCCCAAGGTGAAAAAAACAGACGGCTTTTTGACGATTTTGAAATTAACAGCAAGTTGTAACTTTTAGTGATTTGAATGAAAGGAATATATGACCTTTATTCTTTTAAAAAAAGAGATACATCAATTGTGCTGAAAGCCATTGCGTTAACATAAATAAATTAAGACATAAAAAATGAACACATTAATCAAATAAGTGTGGGAACAGAAAATTAGGCTTTCAACACAAAATTTTCGTTTTCCTGCCCCCACTAAATAAATTGAGTTGGGTTTTAAAAAGCGTCAAAAATTTTAATTTAGTTTTTGCTACTTTCTCCTCCCCCTCACGGGTGAAAACCCCGGCGCTTGGGCCTTAGTGCGCGATAACATTGCCAAGGGCATCACTGCCGGTAGTAACACCCAACAAACCACGTATGATCCCACCCGAACCGAGGCCACCTTGACCACCGCCACCACCTTTGCGTTACGCCGGTATGACCTCGCCGGGCGCGCCTTATACGACCTCGATTTTTCGAAGTTAAACCCACAAACGCCCACGCGCGACCAAACCGGGCAGATCACCTTTAACCCCTTTGGCGGCTTTGGGCTGAGTGGGGCTGCACCCCAACAGTGAAACGAGGTCAAGGATAAGGTCCCCGTCGAGGTGGCCCAAGACCCCTCCAATCCTTATCGGTTTGCCGTTTTACTCGTGCCGCGTAGCGTGGTGTACTATGAGCAGTTGCAGCGGGGGTTAGCGCTCCCTAACCAAGGGAGTTCGTCAGGCTCCGGTCAACAAAACACCACCATTGGCGCGTATGGGCTGAAGGTGAAAAACGCCGAGGCGGACACCGCGAAGAGCAATGAAAAACTCCAGGGCTATGAATCCAAGTCTTCCAATGGATCTTCAAGCACTTCCACCACCCAACGTGGGGGTTCGTCAAATGAAAACAAAGTCAAGGCGTTGCAGGTGGCGGTGAAAAAGAAATCCGGGAGTCAGGGCAACTCCGGTGACCAAGGCACCGAACAGGTGGAACTTGAATCTAATGATTTAGCCAACGCCCCGATTAAACGAGGCTCCAATAACAACCAGCAAGTCCAACTCAAGGCGGACGATTTTGGTACTGCCCCTTCCAGTTCGGGATCAGGCACCCAAGATGGCACCCCCACCCCCTGAACGCCGTGGTTAACGACTGAGCAAATTCACAACGACCCCGCCAAATTCGCCGCCTCGATCCTCATTCTGTACGATGCGCCTTATGCACGCAACCGTACCGCCATTGACCGCGTTGATCACTTGGATCCCAAGGTGATGACCGCGAACTATCCGCCCAGTTGAAGAACGCCCAAGTGAAACCACCACGGTTTGTGGGACTGAAAGGCGCGCGATGTTTTGCTCCAAACCACCGGGTTCTTCAACCCGCGCCGCCACCCCGAGTGGTTTGATGGCGGGCAGACGGTCGCGGATAACGAAAAGACCGGGTTTGATGTGGATAACTCCGAAAACACCAAGCAGGGCTTTCAAAAGGAAGCTGACTCCGACAAGTCGGCCCCGATCGCCCTCCCGTTTGAAGCGTACTTCGCCAACATTGGCAACCTCACCTGGTTCGAGCAAGCGCTTTTGGTGTTTGGGATTTGTTTGTCTTAATTAACTAACAAAATTAATAAATAGGTCTATAGGTTATATTAGTACACCCCCTACACGCCCTTTACGACACCGCTCAATGGGGGGCTGGATGTAGTGAAGGCGGCCAATTTACACCCCTCATACGAACTCGTGGACTGAAAGCGGGTGGAGGATACCAAGTTGGTGGCGCTGGTCCGTTCAGCGTTGGTCAGGGTGAAGTTTCAAGATAGTTCGAGCCAACAAGGCACTGAGAGTAATGGCCAAGACCAAAATGCCCTTAGTTTTGATACCACCAAGTCCCAACAAGCCTTACAGCAAGCTGGCGATCAATCAGGAATTTCATCTAATTCCACCCAAGAAGACTTCGCCAGCTACATCCTCATCTTTAAAGCCGCCCCGAAGGCCACGTGGGTGTTTGAACGGAAGATTAAGTTGGCGTTGCCCTACGTTAAGCAGGAAAGTCAGGGTTCCGACGATCAAGGTTCCAATGGTAAGGGCTCCCTCTACACCACCTTAAAAGACCTCCTCGTCGAACAACCCGTGACCCCTTACACGCCCAATGCGGGGTTAGCCCGGGTGAATGGGGTTGCTCAGGACAAGGTGCATGGGTTGGAACAAACCACTCAATGAAACCAACAAGCCTCCCAAAAAACCTCACCAACAACCCCGCCCCCAAGGCCGTCACCGGCTTTAAGCTCGACAATGGCCGCGCGTACCGGAAGTTAAATGAAGCTTGACCGGTGTATGAACCCCTGGATTCGACGAAGGATGGCAAGGGGAAGGATAAAGATGGGTGAACCACTTCCGGGGCGAGTGAGGCCAAAGGAGATGCCCCGTTGGTGAGCTCAACCGGGAGTCAAATGGCTGCAGTCACAGACAGTCAGCAATCAGGTCATAACAGTGGATTGGTATCGCTTGCCCAGCGCTCCACTACTGTTGCTGTCCAAAAAAGTGACTCTTCTGGTTTTCAAGGCCAAGGCACAACCGACAACAAATTCCAAAAATATTTAAACACCGCCCAGGCCTTGCACCAGATGGGGGTGATAGTGCCATCTTTGGAAACGTGACCTGGTAAACCAAGTACTGGAATCGCTACCCGTGCTGGTGGTGGTGTTTCAGTCCAAGCAGCGACCCGGCAATCGTCTTCTACAAATGAAGATCTGCCGAATGTAATAACACAGTTATACCACACCTCCACGTCCCAACTCGCTTACTTAAATGGCCAGATCGTTGTGATGGGTTCAAACGCGGTACCGAGTTTGTGGTATTGGGTCGTGGATGAGCGAACCACGTCCGGGAGGGCGACTTGATGAGCCCACACCGAGTTGAACTGGGGCACCGACAAGCAGAAGCAGTTTGTGGAGAATCAGCTTGGTTTTAAAGATGACTCAAATTCTTCCTTGACAAACTTCAAGAGTCAAGGCTTAACTCAGCCAGCTTACCTCATCGCCGGCCTTGACGTTGTCCAAGACCACCTCGTCTTTGCGGCCTTCAAAGCGGGCGCGGTGGGGTATGATATGACGACTGATTCGAACGCTTCCACCAAAGACCAAGCGCTCGCCTGGTCGACCACGGCCGGGTTGGACAGTGCTGGGGGGTACAACAACCTGGTGGAAAACACGGCCGGACTGAACGGCCCGATTAACGACTTGTTTACCCTGCTCGACACCTTTGCCTATGTGACCCCGGTGAGTGGGATGAAAGGGGGGAGTCAGAATAACGAAGAAGTGCAAACCAAGTATCCCGTTAAGGATGATAGTAAGGCGAGTGCAAAGATTGCCTCCTTAATTAATGCTTCGCCCTTAAACAGTTATGGGGATGATGGCGATAAAAAAGCCATCTACCCAGAGGTAAGAGGCAGACAGCTCCAAAATATTTTTTGATGATTGGAAAACTAACGCAAGGTGTTAGTTTGATGTAGAGGCAAAACTATGAATTTACTAAATTTTATTTAAATCAGCGGGAGCAGGCTCGTTTTTTCAACACAAGTCACTCTGTCTGCTCCCACTAAATAAATTGAGACAGATGTTCAAAGTCGCCAAAGAGTTTGAATATTTTTGCAATTTGGGTTTGTAATCCCTTCTAACTATTAATAGGAACTTTTACTTTCAAAAAAGTTTTGAAGTTATTTTGGTGTCTTTACTAACTTTAAGCAATTTAGTTAGTGTAAGCACAAAAACCGTTATGTGTAATGTTTTTATTAATTTAGCAAAACAGCTGAACTTAGTTGGCAGCACTACTGCAAGTTGTAGTTTTCTCAACCTCTTTCAGAAAGGAGGTATTGCTTAATGAAAGAGAAAATTTCCGAAAAGGAATACAAAGCTTTAATCCGTAAAACTGGCAAAGAGCACTTTGATGGTGAAAAAGAAGAGTATGGTGATGGCACAGTTGGAGTTTGAACTTATGAATTGCGAAAATATAAACTTAAACCACCTGTGAAAGTTAAATACGTAACCCAAGAACAATTTCAGGAATATAAAGATTCCAACAATCAGCGACTCATAAAGATTGAAAATAAGGTTGATAAATTAGTTGAAATTGTTCAAATTCACGGCGAACAAATTAAAGCACAGGGAGAAACCCTGCAATTAATTTTGCAAACGCTGCAAAAGATGAGTGATCGCTTGGACAAGATGGAAAAAAGAATAGACAAACTTGAGTCCAAATAACTAAAGCCCAGAAATAAAAAGTCATCTTATCAAGGCGAAAGACGGACATCTAATGAATTGAAATCAATTACTTAATTAAATATTCCAAGTAAATAAAGAGATTAATATCTCTTGAGTAGCCGCTGGTATTATTAGCATAGCTACTCAAAAATTGTTTCACACTTTTTTGACTAACATCATCTTTGCCTTTTATTAATCAGCTAAGTGAAGCGCTAAACTCAATTCACTCTAAATTTCTTGTAATTTCTTTTCCATTAACTTTATTTTTTTCTTCTTTAGTAAGAAATTTTTCTGTAGTAAATTGTTTTTTTGGATCAGAATTAGATTTAATTTTAAGGAAAAGATCAAAATAATGCTTGTCACCTTTCTTAGCAGTGAAACCACCTAAAGAATAATCTCCAAACCAATTAAAGTTATAGCTCAACTTAGCTTCAATTGACAAATCCTTAAAACTTTGGTATGTCATTTGAAAATGATCAACCTTTCGCTCAAACTTAAAAGGTTCACTGGTTTTGGGATAAATTACATCTTCAAAATCAAGATCCGGAATACCTAATTCGCCCTTTTCAAAACTCTGTTTTCAGGCGTCTAACAACAATTTTGTTGTATCTTTCGGATTAACTTCGTAAGATTTCTTTAAAGAAGTTATTATGTTCTTTTTTCCTTCTGTAGTTTTAATTGCCTTGTAAACATCAATGTCTTTTTTGGTGGTTTCCTTGATAAGATTTCTGAGATCTGCTTGGATGCCAGTATAGGCGCTTAAAACTGTTCCACATAATAAAAATAGAGAACTAAAGGAAATCCTTGACAACAATCTCTTTTTGCGCATCAACAGTAAAAATAACTCTTTAATTTTGTTTTTTTTTGGAAAAGCAACTTTAAAAACGGAAAGCTAATTGAAATTTCGGTTTGAACATTAATTAAAAAACTGAAATTTTCAATCATTTATTAACAACTATCACTGATAAACATCTCGATCTGAGTGGTTCTCATAATTGCCAATTGTTGGTATTTTTATTCATTTTCAAGCTGTATAAAACTTTTTTGCAATTTTGTTGGCACTTCCAAAAGCTCATTTCAATTTATTTAGTGGAGGAGCAAAATGCAAATCTTTAATTTTTGAAATTGATCAGTAAAGAATCTGATCCCTGTTGGCAACCGAGTTGCAAGCGTTAGTTACTTTTTAAAAAATCAAAAGAAAGGAGGATTTGACCATTAAAAAAGTAAAGGTTACAGAGAAAGCTTCTGTTGAAAGAATGATAGAAGTATTAAGTGATTCTGAAATTTTGTCTTATTTATTAAGTAATAAGCACACTGCTGATGGAGTTAATGAAATTTATGTAACGATGAACATTAAAGACAAAGATTCAAACACTCAAAGGAAGCCAGATAAAATGTCTTTAGAGCTTGAATTGTTTTTAAATCAGTTTATTGAATTCTTAAAAGAGCAGAGAAAAAGCTAGCAGCATAAAAATACCAGCAGTTAATGCATAGCTGTTGGTATTTTTACTTAAGTTTTGACCATTTTGAATGGATAAAATTTCCTTGCAAATAGAAATTACAAACCAAAAACTATAACAAAATGAACTAAGCAATTTTCATCTTGGCTTTTTTCTTATTTAAAAGAGATTAAATTTTCATCTTAAAAGCTTTTATTTTTACCACACAGGATCTAATAAATTTCAAGAGTTAAACAAGTACAAACTTTGTTAATATTTCTCCAAATTTCGGGTTTTTAGTGTTTATGAAATTTAAGTATGGTGCCATCTTTTTTAGTGGCTTTCTAGGTCTTTCTGCCATTTTGGCTGCCTGTGGTACAAAGGGTAAATTTGATCAAGTTGATGATGGCAAGATTAAATTGGCTTCCTCTTTAACTTCTAAAAGTGCATCAAAAGCCTTACAAGCAATTGTTAAAAAATATAACGAAGTTAAAAAACCTGGTGATTACCCTATTGAAATTACCCAAATCGCTGGTGGTTATGATGGTGGTCGTAGCGATTTACAAACCCGAGTCAACGTTAAAGACACCACTAACTTTTACAACTTAATCTTAAACTACCCTGATCTAGTTTCAACTTTAGGTCGTGTTGGTATGGAACTGCCGTTTGACAATGTAAAGGTTGACAAACTATCACCCCGTTTTTTAGATTTCAACAACCGCATTAGTGCAATTTCTAAACCAGGAATTTACGGTATTCCGGTTTCTTTATCCACCGAAGTTTTATCGATCAACGGACCGGTGTTGCACTATATTTTGAACAACGCTAAAAAGAAAGAAGGTACTTTAAACCAAAAAATGACAAGTTCTTCTGAAGGAAAAAACAGCAGTGGCACTTTAACAGTAGCAACTGATACTGAAACAAGTAGTTTATGGAAAAAGATAGAAGATTCTGCAAAAGCTAACGGTAAAAGCGATGAAAAAGGAAAAGGTAAGAAGAAAGATAATAAAAGTGCAACTTTTTCGCTTGTACAACTAAAACAAACTCAAGAAAAAACAGATGATTCCCAAGACACTAAAAATAGTGATGATCAAGTTAAAAAATCTTGAGGCGAATACCAAGAAGTAGATGGTGTTCTTAAAAATTTTGAATTTAAAGCGAGCATCTTCGAAAATTGACATGACTTACTTGATTTTTCTACTAGAGTAGCTAAATCTTTTAAGAAGATACACGAAAATAGCAACAAAAAAGGTAATGACATTCAGGGCATACTTGGAGTCGACAGTACGCCCAACTCATTGTTTACCTCTGTTTTTGCAGCTGGCGGCGGTGATTACAATAATTTCTTTTACAAGATTGAAAATGGTCGCGCTGACTTTAGTAACTTTAAGAATAAAGGGACTTCTTACCAAAATCTTCAAAAAGTATTTGGTGATTTCAAAGGCTTAATTGACAAAAATGGTATCTTTGTGAATAAGGGTGGATCTTACTCATCAAACTTCCAAAAGTTCCACCAATTAGCTTACAGCATTTCCTCTACTTCCGGTTTCTTCTATTCGTTTGCCGGTAAAAGTGCAAAGCGTTTAAATTTTGGTGATAGCTTTATTGAGTATCCGCGGTTTACTCAAGAAATTAAAGCACCATCAAAAAATGGAGAAAATGGCCAAACAAATGAAGGAAATAGTACCAATGGCGAACAAAATCTCTTAGGAACTTTCGAAGTCAAGGATGACAGTAAGCCTAAAGAAGAAGTTAAAAGTAATAAAAATAGTGGTAAAGAAAGTAGCCAAAATCAGGGTAAAAAATCTAACAATAACAAGACTATTTACCTTTATGAAACAAAAATTCCAGATGGAAAAACTGCAGGTGATAATGCTATCTTAATCAAAGATAAGAACGTTATCGAAAAGCTTAAAAGTGCAGCTAAAGAAGAAAATAAAGAACAAACTGCTGAAGCAACTAAAGCTGCAATTACAAGTAATAAAGCAAAAAGCACTAAAAAAGAAAGCAGTAAAGTTATCGGTTACACCACAACTGATAGTGTTCGCGAAGATGGCAAAAACATTTTCGCAATAGATAGAGTTAATGGTGAAAACTATGACCGTAAGATTATTGTAGGTGCTAAAGCAGAAACTTTAAACCAATCTAGCACCTTACAAAGTGAAGAAGCGATTGTTTTGCCTGCCCCTGGAAAGTATCTAAATGGTGATCCCAAGAAAGTGACAATTACCCAAGGACCTAACATCATTGGCATTCATGCTAACGAAAAGGAAAATGCAGAAACGCAAAAGTTCGTAGATTGATTCTTAAACAGTCCGCAAACTTGAGAAAAACAAAGTAGAGATAAGAAAGGATCAAGCGAAAAACAAACTGCTGCTGAGTTCTTTGCGGAATCCGCTTCTTACATTCTGCCCTTAAAAGAAATCTTTGACAAAAACGATACAAAAACAGAAAAAGGTAAGAACAGTAAAACGCAACAGAGAACTAATACCTATGCTGAAAAAGCTTTGGAACTCTTCAAACAAATTAGTCAAAATCAAATAGTAAGCTACAGTGATCCAAGTGACTTTAGGAGTGGAAAATTCCGTGATGCAATTGGTGCTACTTTCAATGCTGCAGTTAGTTCCAAAGCAGACTTCAATAAATTCGTCCAAAACTTTACCGCTACATTAGGTAGTGACATTTAATTCAAAAGCAGTTTTTCAAAATTCTTGATTTACCTTTAAAAGTATCAGCAACACAATAAAATTAACTGTTTGCAAGAGCTCTTTTAAAGCCTTTTAAAAGAAATTAAACTTTTATTGTTGTGCAAATTAGGACTTACAAAATCAAAGATATTTGCGACATTCAACGTGGTCGTGGTATCACTAAGGAATACATTAAAAATAACTCTGGAAAATATCCTGTTTATTCTGCCGCTACTACCAATAACGGTGAGTTAGGTTTTATTAATACTTACGATTTTGCTGGTGAATATGTTACCTGAACCACTAACGGCTATGCTGGCGTTGTTTTCTATCGTAATGGCAAATTTAGTGCATCACAAGACTGTGGTGTTTTAAAAGTAAGAAACAAGGAAATTAACGCTCAATTTTTAGCTTTTGCTTTAAGTTTAAAAACACCTCAATTTGTCCATAATTTGGGATCAAGACCTAAATTAAACCGAAAGGTGGTAGCTGAAATCTCCTTAGATTTTCCACCTCTAGAAGTTCAAGAAAAAATTGCTCATTTTCTCAAATCTTTTAATGAATTAAGCAGCCAGTTAAAAGCTGAATTAATTAAACGACAAAAGCAATACGCATTTTACAGTGATTATCTACTCAATCCTAAACATTCACAAGGTGAAGAATACAAACTCTTTAAACTCAAAGATATAGCTAAAAAGATTTTAGTTGGCGGTGAAAAGCCAAGTGATTTCCAAAAAGAAAAGGATCAAGTTTATAAATACCCTATTCTTTCTAATAGTCGCAAAGCAGACGATTTTTTAGGCTACAGCAAAACGTTTAGAATTGCTGAAAAATCAATTACAGTTAGTGCAAGAGGCACAATTGGTGCTGTTTTTTACCGTGATTTTTCCTACTTACCCGCTGTTAGTCTAATTTGCTTTATTCCTAAGCCAGAATTTAATATTAATTTTTTGTTTCATGCTTTAAAAGCGACTAAATTCCACAAACAAGGTTCTGGCACAGGACAGTTAACCATGGCACAGTTTAAAGAATATCAGGTTTATATTCCATCTTTAAAAAAACAACAAGAAATTGCTGCCACTTTGGATCCACTTTACTATATCTTTGCAAATTCTAATTGAGGAATATACAAAGAAATAGAATTGCGCAAAAAGCAAATGCAATACTATCAAGAACGTTTGTTTCAGTGAATAGAAAACCAAAAAGTTTAATTACTAGTTAATTTTATCCGTTTAGATTTAAGCGCTTAAAAGAACAAAGTGTAAATAGCTGGCTAGATCAACTACCGGTGGTTTGGTAGCTCCGGTGACTAGTTCTAAATCATGCAAGTTTTGTCCTCTCAATAAATTCATAAAGTTTGGATCGGCAACTGAAATCTGTTCTTTGACATACAATGTAAATAACAATTCATCCTTGTCATTAAACTGGAAGAGAGAACCAGGATAGTCTTTTTTAGCAACGGTGTAAACTGGAATTGATCCATCAACAATGAACTTTAAGTTCATTTCACCACTTCAAGAGCTTGGGAATTTAGTAAATAAAGCTGCAAAAGCATAGAAACCATACAATTGGTAACCACCGGTATATTTAATGTCCACTTCAAATCGATAATTAAAGGGATTAGCTTTATCTTGTTTTATTCCAACACCATCTATTCAATTAATGGTTGGACTTTCAATTTTATTTTGAACTTTTAATTCCTTAATCTTTAGATTGATGTTGGATTTGCTAGTAAGTTCACCGCGCAATCAATCGGACATGTAAATTCCATTTAAACCATCTTTAGGGAATTGGATTCCTTGACTACCAAAGGCCTTTTGCTTCGCATCGTTATTTTCTTTTTTAGCGTTGTAATACCAATCAATGGCAGTTCGAAATCCAGCAATTAAGAAACTGAAAGTTCTGTTTCTATCTGCTTCCAAGGAAATTTTTAAAGCCTCTGCTAACTGTTTTTTATCAGTGACATCTTTGTTCTTCTTACTTCAATATTGTTTGAATTCTGATGCAGCTGTTAGAGATTTGTTAAAAGCTTCTCTTTCTGCTTCGAGTTTTTTAACTTCCTCTGCTTTTGCTTTGGCCTCTGCTTCTTGAAGTTTACGCTCCTCTTCTAGTTTCCTTAACTTTTCAACGTGCTCAGCATGTGCTTTTTCTCTTTCTTGCTTGAAAGAATTGAGTACTTTTTCCCTAAATTTAGTTGCAACATCTGTTTGGTTATTTATTTCAATAACTGATTCTGTCAGGTTAACTTTAGTATCAAATAAAGAATCACCTAAAGCAGTTTGAGCAAGTGTAGTTAAATCATTTGTGCCGTTTGAAATTAGCTTATGCACTGATGGTTTTCAAAACAGTTGGGTATTTAAATCTTTACCAGATTGTCAGAAACTGTCTTGTAAATCTGTTAGATTGGCAAAATTAGTAGTGGATGCGTTGAGTGCAAACTGTAAATCTATCGTGCTACCATCACCTTCATAAATAGTTGCAAAATCCATTTTGATGGAAAAACTATAACAAACTTTGCCCTGAATGACTATGTCGTTTTTTCGCTCTAAATTTAAATCAAAGTTTTTGAGTGCAATCGACTTAAGGTTATATTGATCGAATAATGCAGCAATATTGTCAGCAATCTTTAAACCCTTTGGATGTAATTGCGGAAAGAAACCATCACCAAGTAAACCAAAAAGCTTTTGTGACACTTTAGGAATTTTAGTGCTGTCGGAAACAGGAAACACAGCATTAGCTGCATTTATTTCTGCTTGAAGTTGATTCATTAAATTAACCGTGTTTTGCACACTTTGTTTAAAACCACTAAGAAGGTTATAAACATCAATTAAACCTAATGTTTTTGGACTTAAAGCTTGTTTGACAAAGTCCTTTTCAAATTGTTCAAAGTTGTGCTTTCAATCCTTATTTTTCGCTATTACAGCTAACTCTTCCTCACTAAAAGTAGATGCGTTAGCAAGATTGACCAAGTAGCTGTCATTATGGGCTCTAGCTAAACTTTTATTGTGTAGTGCTAAAGTATTAATGTCAGCACTAGAACCTTTTTGAGTTAATGTAGGAAATAAAAATGCGCCTAAAGTTACAGTAGTGGTACAAAAAGCCAGCAAAAACGCTTTTGTTTTGAAGCGAATTTTTTTTAGTTGCTTTCAATTTAGTTGAGAAATAAGCTAACTAAATTACTTATTAAACTAACGAAATCAATTAAGAATTAACTTTCAACCACAACAAAGTACTACAACAAACAGGGAAAATGTAATTTGTACGTAGTATTTTAGTTCTTTCTTGTGAAATATTGCAAAAGTTAGCTAAATCGAAATTAGCTCTTATCCGCTAAAATGAGAAAGTGGAGGTAGCTCGCTAAGTCTACTACTGGCACTTTAATGTCACCACCTCAAGGCTCTAATACATCGAGCTGTTGTTTCTTCAATAATCCTTTAAAGCGTTGATCCTGTACATTAATCCACTGAGTAATGTGTAGAGTAAATAACAGCTTGTCATCCCTAAACTGGAATAAGGTTCCAGGATAGTCCTTTTTGGAAGTAATTCAATCTGCTAATTGTCCGTCAACATTGAGCACTAATTCCATTTCACCCTTCCATTCTGGAGGAATTCCACCAAGTCCTCAATTTGCTAAAGTTCAGTAAATACCTTTAAGTCCATAATCACCGGTGTATTTCATTCTGATTTGGAAAGCATATTTAAACGGATAGGTTTGTCTAAACTCAATACCGTTCTTGGCTTCATTTCAGAAAATTTGTTTTGGTACACCCCAACCATTAATGTCATTGTGAATATCTTTTGTAGTTACCGATTGCAGGTTCAACTTTAAGTTAGCTCTGTGTTTTAGCTCTCAGCGTAACCAGTTAGGGATAAAGATTCCATCAACACCTAGTTTTGGTAATTCAATGCCAACATCACCAAAGGCTTTTTTCTTGGAATCTTGATCATCCTTTTTGGCGTTGTAGTACCAATCAACAGCATTTCTAAAAGCATCGGTAAGGAAAATAAAAGTTCTGTTTCTATCAGTCTTAAAGGCCAGTTTAAGTACTTCAATAAGTTGCGATTTAATTTCAACGTTTTTACCTTTTCCTTTTCAGTAATCTTTAAAACTTTTAACGTTATTGAATGAACTAAGAAAGCGTGTTTTTTCCCTAATTAACTCTTGAACTTTCTTTTCTTTACCTTTAAGTTCTTCTTCAAGCTGTTTACGTTCACGTTCTAAACGCGCTTGTTCTTCATCATGAATGCGTTTTGCTTCCTTACGTTGTTCAATGAATGGATTGAGGATGCGTTCCTCAAACGATTTGCGAGCTTTAGTTAAAACCGTTCCATCAATTACTGAATCAGTTAAGTTAACCTTAAGATCAAACAGGTTTTCACCAATCGCTGTTCTTGCAATTGTGGTTAAGTCATTGCCCTCATCAGTAATTAATTTATCTATTACTGGTTTTCAGAACAATTGGGTTGAGAGTGTTTGACCACTATTAGTAAAACTTTCACGTAATTCCTCTAAAGAGCTAAAGTTATTCGTTTGAGCTTGCAGAGCAAAATCAAGGTCAATTTTGGTATTTTTGCCAACATAAACGGTGTGAAATTTCATTTCGATGGCAAAACTATAGCGCACCTTGTTCTTTAAAACAATGTCGTTTTGTTTGAGCAACTGCAAGTTAAACGCTTTTAACTTAATTGAATTTAAGTTGTAGTCATTAAACAAAGAAGCAACCGTATCACTAATATTTAAACCACGTGGTGTTAATCTTGGGAAGTAATCTTCACCAAAAAAACTAAAGAAACCTTCTGGTACTTTAGGGATCGTGTTTTTGTCCTCTACGGGAAAAATCTCGTTAGCCGTATCCGCCCGTTTTTGTAAGTTGTTTGCTAAATTTACTAAAGCTTGGGCTTTTTGTTGAAACCCACTAAAGAGGTTATAAACATCAATGAGAGTAAAAGCTTGGGGATCAATAAGTTGGTCAGCAAACTTATTTTGGAACTCGCTAAAGTTTTGTTCTCAATCAGTGCGACTTGTAATCGTTTCGAACTCGGTTTGACTAAAAGACGAGTTGCGCTGCAATAAGTAACTCTTGTTGTGTCGCTTATCAAAGCTACTGCTATTTAAATTAACAGCACGTGGATCATTAGCTGAACCGTTCTGTGATAAGGTGGGTACTAGAAAAACGCTAAAAGTTAGCGTTGCTGAAAATAAAGACAGCAAAAACGCTTGCGTCTTGAAGTGAAAGTTTTTCGAAGATTTCAAAGATTCTTGTCAGTGTGACTAAAAGAAATAAATTAACTTACAGGTTACGAAATGCAGACAAAATAAAAGAAGCAACGGAAAATTTAAATTCAGAAAATTCTAGTTTTTTTTTTTTTTTAGTTTGAACTCAAAACGGGTTGGTGAAAGAAACTGCAATAAGCTGTTGTAAATAACTTTAACTAAGTTAAAAACTGTTTCAAACTAACCAAGCTTACTTTTAGTGTGCCTTATTGGAAATTAATGTTGGCTTAATTATTTATCTGCCAAAATTAAGAAGTGGAGGTAGCTCGCTAAATCCACAACTGGTACTTTAATTTCACCACCTCAAGGTTCAATCACATCCAATTGCTGTTTCTTTAGCAGGTTCATGAAACGTTAATCCTTAACGGTAATACGTTGCACTACGTGTAGGGTAAACAACAGTTTGTCATTTTGAAATTGGAATAACGAACCAGGGTAATCTTGCTTTTGGGTAATCCAGTTAGCTAGCTTACCATCAATTTGGAACTTCAATTCCATGTCACCACTCCATTCTGGCGGAATGCCACCAAGACCCCAGTTAGCCAAAGTTCAGTAAATCCCTTTTAACCCATAGCTACCTTTGTAATTCATGGTTATTTCAAAACCATAAGTTCAAGGAGTGGTTGCTCTGTAATTAATGCCGTTGTTGTGGTCGTTCCAATTAATCGAAACAGGCACACCTCAACCATTAACCTCAAAGTTGTTTTCCCTTACTTTGACATTTTGTAATTGAAGTTGAATATTAGCTCTGTTGATTAATTCCCAACGTAACCAGTTAGGCATAAAGATGCCGTTAAGACCTGATTGAGCAAAGGAAATGCCGCCGCTACCAAATGCTGTACGTTTTGCTTCTTCATCGTGTTTTTTATGGTTGTAATACCAATTAATAGCATTCCTAAAGGCATTGGTTAACAGTTCGAAAGTAGGGTTCTGCTTGGCTTGGAAGGAAAGCTTTAAGGCTTCGATTAGTTGCTTCTTGTCTGCTACATTTTTACCGCGCTTAGCTCAGAACTCCTTAAAACTCTTAACGTCTCTAGTAGATTGGTAAAAGGCTCAATGTTCTTCACGAATACGACGAGCTTCATCTTCCTTGGCTTTCAATTCAGCTGCAATCCGTTTTCGTTCCGCCTCTAAGCGTCTTGCTTCTTCATCATGAATGCGCTTAGCTTCAACTCGTTCAGCGTGGAACGGATCTAAGATCTTGTCCTCAAATGATTTTTGCGCTTGCTGTAAAACGCTTTGATCAATTATTGAATCAGTTAAGTTAACCTTGAGGTTAAACAAACTTTCACCAATTGCTGTTTTAGCAATGTTGGTAAGATCATTGCCACCATCTGTGGTTAATTGTTCTACCACTGGCTTTCAGAATAGATGGGTTGAAAGAGTGTTGCCATTGTTTTCAAAACTATCACGTAGTTCTTGTAAGCTAGTGAAGTTTATTGTTTGGGCTTGTAAACTAAACTTTAAAATAGTGGTGTTCTTTCCAGCGTAAACCGTGTCAAAGCCCATTTGAAGAGCGAAGTTGTAACGCACCTTATTCTTTAAAACAATGTCGTTTTGGCGCAACAGTTTTAGGTTAAAACCAGTAAAGTCAATTGAATTTAAGTTGTAGTCATTAAACAAGGAAGCCACATTATCGGCAATGTTCAAACCACGGGGAGTTAACCGCGGAAAGTAATCTTCCTGAAAGAAACTAAAGAAGTTTTCCGGCACTTTAGGGACAGTAAGCATTGTCCTCTACGGGAAAGATTTCGTTTGCCGCATTAACTCGTTTTTGCAAGTCATTGGCTACATTAACTAAACTGCGTGCTGTTTGTTCAAAACCACTAAAGAGGTTGTAAACATCTGTAAGTGTAAAATGCTCTGGGTCAATTGCTCGTTGGGCAAACCGTTTTTGGAATTCACCAAAGTTTTGCGCTCAGTTAGTTTGCTTGGAAAGGTTTTCATACTCAGCGTTACTTAAAGCAGTTTGCGCTTCAATAAGAATTTCTTACTGTTATGCTTTTCAAAGCTACTGCTGTTTAAACTTACAGTTTGTGGATCAGTAGCTGAGCCATTTTGGGAAAAAGTGGGTACCACAAAAACACTAAAAGTTAGTGTTGCTGAAAGGAGCGAAAGCAAAAATGATTGCGTCTTGAAGTGGAAACTTTTAGTTGGTTTCAATGTAAAGGTGTCAGTGTGACAGAAAAAGATTGGTTTACTTTTAAAAAGTGAAAACTAAGACGACAGGGAAAATTTAAATTTAATAAATTTTAGTTTTTTCACCGAACAAAAAACGGTTTCATCGGAAAATGAAGCATAAACGGCTCAAACAACTTTAAGAATGTTTAATGATGCCTTGATTACTGTTGATTTAACTTCAACAGTTGGTAGTGTAAATAACTCGCTAAATCCACTACTGGCGGCTTTGTGGACTCGGTACGTAGTTCTAAGTTATGGAGGTTTTGTGACTTAATTAATTCCATGAATTCTGGCTCTTGCACCGTAATTTTTTCTCACACATGGAGCTTAAATAACAGCTGTTGCTTTTGATCATCAAACTGGAACAACGAACCTGGGTAATCGGGCTTTTGGACAATCTCTCAACTTAAATCACCATCAACAATAAATTTTAAATTCAATTTACCCTTTCACTCTCCGGGAATACCAGAAAACTTGGCAATAATGGCTTCTAATCAACTTAATTTGTAATTACCTTGATATTCAATGTCAATCTCAAAGCGGAAGTTGTAAGGTTTGTCTTGATTTCGCTTAATACCGATATCTTTGTTCCATTTAATGGTAGGACTTATCTTTGAATCAGTTACCTTTAAGCTCTTTATGTCTAAGTTAATCTTGGCCTTTTCCCCTAGCTGTTTACGCAACCATTCCGACATAAAGATGCCCTTTAAACCAGATTGGGGAAAGGCGATACCTTGTGTACCAAAAGCAGTTTTAGCAGCCTCACTTTGATCTTCTTGGTCATTTGGGTAATAATTGCTAATACCGTCCTGGAAGTTCTTAATCAAAAAGGCAAAGGTCTTATTTTGTTCTTTGGCAAAGGAAAGTTTTAAAGCTTCTAACAGCTGCTTTTTATCGGTAACATCTTTCTTTTGCTTTTGCCAGTATTCTTTAAAGGCTTGCACTGACTCAAGCGATTTGCTTACTTCTGCTAGCTATTCAGCTTCCTTTTGTTTTTGTTTTAATTCTTCGAGCTGTTTTTGGCGTTGCTCCTCTAAGACTTTTTGGTGTGCTTGGTGTTCCGCTAGTGCCTTTTGCCGTGCTTCAATGAACGGTTTTAAAACAGTTGCTTCAAACCTTGATTCAGCCTCTTTTAGCACGGTTTGATCCAAAACTGACTTGGTTAAATTAGCTTGGGTGGTAAATAAATTATCACCAACAGCTGCTTTTGCTACAGTTGTTAAATCATTGTTATTACCCAACACTAACCGCTTGACCGTTGGCTTTCAAAAAAGTTGTGTAGTTAAGTTCTGGCCACGTAAAACCAAGCTGTCTTGCAATTCCTGTAAGTTAGCAAAATTAGTGGTCATTGCCTTAACTGAAAAAGCTAAGTTGGTTGTACTGTTTACACCCGTATAAACTGTTTCAAAATCTCATTGGATTACGAAGCTGTAACGCATCTTGTAGCGCTTCACAATGTCCGGTTGTTTTTCTAGTTTCAGATCAAACTGCTTTAACGCAATGCTTTGAAGTTTGTATTGTTGCAAAACTGTCGTAATATTCTCAGTCACATTTAAACCCTTAGGGGTTAATTGAGGAAAGAAATCACTACCCAAAATGGTAAACAACTGTTCGGGTACTTGGGGCAATTGAAATGCCTCGTGCACCGGAAAAACGGCATTTGCTAGTAATACCTTTGCATTTAAGTCATTAATTAACTTAACGGTATCACGCGTACTTTGTTCCAATCCACTCAACAGGTTGTAAACATCAATCACACCCAACTGTTGTGGGTTAAGCGAATTGTCCTTAAACTTAGTTTGCAACTGTTCAAAGTTAGCCTTTCAGTTAGTGTGTTTGGTGGTGTAATCCAGGTCAGCTTGACTAATACCAGAATTAAATTGCAACTGGTAGCGGTTGCTTTGGTTTTGTTCAAAGCGTTGGTTGCTTAAGGTAAAGTCACTAACTGACTGGTTTGTTTTTTCAGTTAAAGTCGGTCAAACAACGATAGCTAGAGTAGTTGTTAAACCAGCTAAGCTTAACGATAACGTTTTGAGAAGTAAACGTTTCTTTTTAATTAATTTCAAGTTTGGTTAAAAACGGTTTAACCAAATAGTTAAATTACTAACCGAAATGAATTAAAGCAACTACAACAACAGAAAAATTTAATGCGTTAATAATTGTAGTTTTTGTACTTTACTGTGTTGACAATCAACTGTTAAAAACAAATAATACTGTTAGCTTTTATCGATTTGTTGCACCAGCCAGTCTTTGATTTCGCCATAATAACTAGAGATACTGCGCACTAGTGCTTGACCCACTTTTAAACTTTTAATTTCACTAGCACAGGTAATATCAACCCCATACAGGTTAGCCACTTCTTCTGCTTCCATCATGTCAATTAAGCGGTGGGCAATTAGGGTACTGGTGTTGTTAATAATCTGTGATTTAAAGTTATCACCACCAATATTGCGCAGGTTAGCAAATGATTGATAGGACAGGATAACTACAGTATTGAACGAGCGGACTTGCTCCAATACCACCCCCACATAGTGGCTCCCAAAATTACCAAATTCATCAGCAATAAAAAAGGTCGAGTTATCACTCGTTTCTTCCAAACTCGTAATTGTTTGGCCAAAATCCAAAAAGATTAACTTGCCCAAGTACTTGGCATTAATCATGTCGTACATGCTGCTAATGGAAAACAAAACAGCATCTTGGCAGTTTGATAATATCTATCAATCCCACACCATTAGAATCGATTGAACCAATTTCCGCTAGTTCCCTGAGGTTTTCTCTTAAATTTTCAAAGTCCTTTGTGTCAATGGTTTGAACATGCTCCGTATAGTATTCATAAATTTTTTCTACAAAAATTTGATTGGAAGGTTGAAAGCGACCTTTTTCTTTCATTTCTAAAATTTCTTTTCGGCGTTTTTCTTTGTGAATTTTTTTAAACAAACTGGGTTCAAATTTAGCAAAAATATCAAAGTCACGTAGTAATACTTCATTTGTCCTTTGCTACAGTAACGCGCTAAATTCTTAAAACTCAAATTGTGGGGACCTTCCTTTGCAATTACTAAAGGTACTAAAACTTGCAAAATGGCCTTCTTATTACGTCAATAATAAGCACTGTCCTTGGTGTTTTCCACCATATCGTTATAACCAAACATTTCCAAGATTAAAGTAATGATTTGTTTGGGTGTTTTTTACTTAAACGGGTTGTACTTAATCGTGGCAATTCTGTCATCTAAGCTCCAAATTTTGGCACTAGTATTTTTGAGTGCCACTAACTTAACCGTATCAAAACCGTCTTCGGGACTTAGTTGTTTGGCGCTCGTTTGGTAGTGTTCAAACTGGGTAATTAATTCCTTATCACCCTTACCATTCACAAAAATTAAGGGATGGTCGTTGTCAATTGCATACTTCAAAAAGTGCAGCAAGATATTGGACTTACCAAAACCACTCACACCTAGTACCGAGACGTGCTGAGGGAGCTTGTCCAGTTCCAAAAAACACTCCTCGTTTAAAAAGATTTTCTTACCAGTCTGGTTATTGATGTCCTGTTCCATTACTAAAAAATAATTTGTACTGCAACTTCAAAGTTAACTTATTCTAATTTTGCTAGATCATTGGTTTCATTCACAGTTTCCCAACTTAGTTTTCACTGTTCAAACAATTAAAAAAGCACCAGCTAGTGCCAGTGCTTCTTTAATCGATAGTTTAGTGGTTTTTAATACTCCTCTTGAACCGTTTTGGCACCGAGGTAGGCCAATTCTTCATCGGTTAAAAACCCAGTACCAGCCGGGATCTTACCCCCAATGATCACGTTTTCTTTCAACCCTAAGAGGTAGTCAACTTGGGTTTTTACCGCAGCATCGGTCAAGATCTTCTTGGTGTCTTGGAAGGATGCGGCACTCAAGAAGGAGTTCGACTTGCTGGCCGCCTCATCGATCCCAAACACCTGGTTAATGGCAATCACTGGCATCTTCCCCGACAGCAACAAGCTCTTGTTGAGTTCATTGAGGTGGTGACTGTGTACCAGTTGACCCACAAACAGGTTACTGCTGCCAGCATCGGTTACTTGGAGTAAGCTCGTCAACTGGCGGATAATAATTTCGACATACTTATCAGCAATCTCAATCCCCTGAATACGGTACACCTTTTGGATTTCGACAATCATGTACTGGCGTACCCGTTGGATCCCAGCAACCCGTAATAACTGGCGAATGTCAATCGAACCTTCGGTAATCTTCGAACCTAGTTCTACTGCATCACCGACCTTGACACGCAGTTGCGCACTAAACGGAATCGAGTAAGTGCGTTCATCGACACTAGATTCAATCAACACTTCCTGCGCGTTTTGGGTGGTAGTAATCGATTTAACTACCCCCTTAACTTCAGAAATGACACAGCGCTCGTAATCCTTAGGGGCGACTACTTCAAAGATTTGCTTTAAACGTTCAAACCCTTGGGCCAAGTTGTTTTCGGTCGAAACCCCCCCGGTGTGGAAGGTACGCATCGTTAACTGGGTTCCTGGTTCACCGATCGATTGCGCTGCAATTACACCCACAGCGGTACCCAATTCCACCAGTTTGCCGGTGGACAAATCGACCCCGAAACAGTACTGACACACGCCGTTTTCGCGTTCACAGTAAATCACAGAGCGCACTAACACTTGTTTAATCGAAGTGGCACAAATTTGCTTGGCTAACTCTTGGGTAATTAGGCTGTTAGCAGGGACAATTTCCGCCTTGGTTTCGGGATCTAAAATTGGTCCAATCGTGTAACGGTTGACAATGCGGTCAAACAGTGATTCCACCAACGAACGGGTTTTGGTTTCCACAATTGCTTCGACCACAATTCCCTTGCGTGTACCACAGTCATCGTGGTTAATAATCAATTCGTGGGTTGCATCCACCAGTTTACGGGTCATGTAACCAGACTTAGCCGTTTTCATGGCGGTATCGGTCATCCCCTTACGCGCCCCATAAGATGAGTTGAAGTACTCGTTAATCGTTAAACCTTCCAGGAAGGAGTGCTTAATAGGAACCTCAATCGTGTCCTTAATAATTTTGGACTGGTTGTTGCGTTCATAGTTAAAGCTCTTACTCATTAACCCCCGCATCCCAAACAGCTGGGTAAAGTTGGAGATATTACCCCGCGCCCCGGAATCGGCCATGACAACAATCGAGTTATCACGGTACTCTTCACGTTTAATTAAGTCCTGGATCTCACTCGACACCTTTTCCTTCACACCGTTTCACAGTTTCACCACCCGTTTATACCGTTCATCATCGGTCAGTAAACCCTTGTTGTAAAACTGCTTGTACTTCAAGACCTGTTGGTCCGCTTCATCAAAGTACTGCTGTTTGTTGGAGTACTTCGGAATATCAAACACCGATACAGTGGTGGAAGAGTGGGTCGAGTACTTAAAGCCTAAGGCCTTAATGTTTTCCAAGATCCGTGGTAGATCCTTAAATTCAACCACCGTGTAAAGTAAGTCAATTAACTTGGAGATGACCTTCTTACCAAAGGCAGGGAGCACTTGGTGTGCGGCAATCGCAGCACGCACATCACCACTTGGTGGTACTATGAACTTTTGCGGACACGCATGTTCATCAAATTCCCCTTCATTAATGTAAGGGATGTTGTCCCCTAACACATCGTTAAAGATAATCTTACCAACCGTAGTGATTAGAGTACCTTGAGCTTCAAAGTGCTTGTTTGGAAAGGCTTTGGTGCTAATGCCGACAATGGCGTGGAGGTGCACCTTGCCCGCTTCGTATGCCGCGCGGGCTTCATGCACGGTACCAAAGATGATGCCTTCACCCGTTTGACCCTTACGCTCAGTAGTGAGGTAATAGTTACCTAACACCATGTCCTGGGTTGGGGTGACAATTGGCCGGCCATCTTTTAGTCCCAAGATGTGCTTGGAAGCTAACAGAATAGCACGCGCTTCGTTAACGGCATTTTCGCTCAACGGAACGTGCACCGCCATCTGGTCACCGTCAAAGTCAGCGTTAAAAGCAGTGGTCACTAACGGGTGTAGGCGAATCGCTTTCCCATCGACAATCCGCGGTTCAAAGGCCTGTATTCCCAAACGGTGCAAAGTAGGTGCCCGGTTTAATAACACCGGACGGTCCTTAATGACATCATACACAATCCCCCAGATCAGGTCGTCCTGATTTTTAATCATGTCCTCCGCTTGCCGAATCGAGGACGCAATTGGTCTAATTTCGTTACCGTTGCTGTCAAACTTATTAATTAACCCGTGAATAATAAATGGTTTGAACAGCTTGAGGATCATTAATGCTGGAATCCCGACCTCATACATCTTTAGTTCTGGACCCACCACAATTACACTCCGACCAGAATAGTCCACCCGTTTACCTAAAAGGTTGTGACGGAACAACCCTTGTTTCCCTTTTAAGCGGTCGGTGAGTGACTTCAAGGAACGTTTGTCTTTGGATAACGCTGGCTTGTGGCGCGACGAGTTGTCAAACAAGGCATCAACTGATTCTTGTAACAAGCGCTTTTCGTTGTTAACCACAATGGCAGGTACGGTACCATCTTCCAAGATGCGGCGTAAACGGTCGTTACGGATAATTACCCTTCTGTAGAAGTTATTAATGTCACTGGTGGTAAATTTAGCACCATCTAACTGGATAATCGGCCGGATGTCTGGCGGGATCACTGGCACCGTGTGCAGGATCATGTTTTTCGGGTGCAGCTTCGAATTTCTAAACCAGCTAATTGTTTCCAATTGACGCAAGATCTTTTTAACCTTAGCATCCTCAACACTATCCTTCTTAGCCTTCCGTAACGCATCATTGAGCTTACTAAACTCATAGTTAAGGTCAATCTTGTTGAGCAATTCTAAAATGGCTTCAGCCCCGATACCAACGCGGAAGCCAGTGTACTTCTTAATGTAGTTAAAGGCATCATTTAAGGAGAACGGTAACGAACTGTTCTTTAAGCTTTCATAAAAGATCTTCCCCTTGCGGTAGTCTTCGCTACTCCTGTTCTTAATCAGGTTCCGGAAGATGTAACCGATCACCCGGCGCATTTTTTGCCGCGTGGTTAAAGAACCCTTGCCTGCTAAGTCCAACACTTCCTTAAACTTAAACGGCATGATCTTGGGATCTTTGATCTTCCCAGTATCTAATACTATGTAGTTAACAAAGTACAAGACCTGTTCCACTTCCTTATAAGAGATGTTTAACACTAAGGAGATCTTGGAAGGGGAAGGCAGTTCCTTCGACATCCAGATGTGCGCTACCGGACTGACTAGGGCAATGTGGCCCATACGTTCCCGGCGCACAATTGATTCGGTTACCCAAACACCACAGCGATCACAGCGCACGCCACGGTACTTAATCTTCTTAAACTTCCCACAAGCACACTCATAGTCCTTCACTGGACCAAAAATGGCTTCATCAAACAAACCACCAGGTTCGGGTTTTAAGGACTTATAGTTAATCGTTTCCGCCTTGGTAACTTCCCCTTCGGATCAGTTTAGGATCGTGTCGTTGGAAGCAATCGAAAGCTTAATTGCCTTAATGTTCTTGTACAGCTTGTTGTTCTTTTTATTACGCTTTGTCATTAATTAATAACCCTCCGTGTCAAATTCAAAGTCATTAAAGAGATCGTCCTGTTCTCCATCAGCTTGGAGAATTGAAACGTTGTTGGAATCTTGTTGGGTGTTGTCATCGTAAATAAAGGACACACTCAACGCTAACCCTTGTAGTTCCTTCGTTAACAGTTTAAACGATTCGGGGATACCTGGTTCGGGGAAGGCAGCGCCCTTAACGATCGCGGCATAGGCCTTATTCCGGCCCTGCACATCATCGGACTTAATGGTAAGTAATTCCTGAAGGTTGTAAGCAGCGCCGTAAGCTTCCAAGGCTCACACTTCCATTTCCCCAAAGCGTTGGCCCCCATTTTGTGATTTACCACCCAATGGTTGTTGGGTAATCTTGGAGTAAGGACCGACAGCGCGAGCATGGATCTTGTCATCAACCATGTGGTTTAACTTCATAATGTACATGATTCCCAAGGAAATTGGTTTTTCAAATGGTAAACCAGTGCGACCATCAATCAGCTTGAACTTCCCGTGCGTTTCCGCTGGTTTCAATCCTGCTTCATCCATGATGTCTTGCAAGTCTTGGAAGTTCACCCCTTGGAAGATCGGGGTCGCTACTTTAAAGTGGAGGTCATCAAAGCTCATGCCGATTTCCTTGAGCACTAAAGCAATGTCACCGTTATTGAGTTGGGCCAAGGCTGCCTGCTCGTCTTTAAGACCGCGGTTCGTAATTTGGGCTTTTAAACCAGTAATTAAGCGGTCAAGTTTGTACTGTGGCTGGTTAATTTCGGTTGAAAGTGCTTGAGCCTTCTTGTCATCAAAACAAGCACTAATTAACATTCGCTTGGCAAGGTTATGTGCTGCATAACCCAAGTGCATTTCAAAGATTTGGCCAATATTCATCCGACTCGGTACCCCCAGTGGGTTTAAGAGGATGTCGACTGGAGTACCATCTTCTAAGTGGGGCATGTCCGCTACTGGGACAACCTTAGAGATAACCCCTTTGTTACCATGGCGCCCTGCCAACTTATCTCCGATTTGGATCTTGCGTTTTTGCACCACATAGACCTTAATCATTTCGATGACACCATCATTAAGTTCATTACCGTTGGCAATGCTAAAACGCTTGACACAGGACACAATCCCATCACCACCATGAGGGAGCTTTAAGGAAGAGTCACGGACGTTTTGCACACTTTCAGGGAAAATGGCCTTAAACAGCTTTTCTTCAGGTGATACTTCAACCTGTCCCTTAGGGGATACCTTACCGACAAGGACATCACCTTCCTTAACTTCAGCACCGACCATGATAATGCCGTTTTCATCTAAGTAACGCTTGTTAGCATCACTAACGTTGGGGATATCACGGGTAATTTGTTCATCACCGTTTTTGGTCGATAAACACTGGGCTACGTATTCGTTAATCGTTAAGGAGGTTAGAACATCATCCTTCACTAAGCGCTCGGAAATCACTATCGCATCCTCATAGTTGTACCCGTTTCAGGTCGTAAAGGCGACAAGGACATTTTGCCCTAAAGACAGCTCACTCTTGTTAACTGCAGGACCATCAGCAATCACTTCGTCCTTAGTTACCCGTTGCCCCTTTTCTACTAAGGGAACGTGGTTGTAGCAGGTATTTTGGTTGGAACGTTCAAACTTAATTAGATCCAAAGTGTGCTGTTCACCACTGTCACTTTCAATCGTGATCTTACTGTTGTCTACATACTTTACCGTACCTGAGCAAGGTGCTAACATCGTCAAGCCGGAGTCGCGAGCAATCTTGTACTCTTGACCTGTACCCACAACGGGAGCGTAAGGCTTAATTAAAGGATAGGCTTGGCGTTGCATGTTGGTACCCATCAATGCCCGCGCTGAGTCATCGTTTTCCAAGAAGGGAATTAAGGACGAACCGATGGACACTACCTGATGAGGGGCCACGTCGATGTAGTCAATCTTCGAGGGATCATACAACCCTTGCATCGAGCGGTAACGACCAATAATTTCCTTATCTAAGATCTTGTTGTTTTCGTCAATGTTGACCAACGAAGAGATTTCGGCAATAATGTGCTCGTCTTCCCTTAATGCCGTGAGGTATTCAACTTCATCGGTAATGACACCATTTTTAATCTTGCGGTAAGGCGCCATTAGGAATCCGTTTTCGTCAATCTTGGCAAAGGAAGCCAACGACATGATTAAACCAATGTTCATCCCTTCCGGTGTTTCAATTGGACAAATCCGACCATATTGGGAATAGTGCACATCCCGGATATCCAAGTTCGGGTCTTCGCGGGAGATACCCCCTGGACCCATGGCGGAAATCCGGCGCTTATTACTTAATTCTGAAAGTGGATTTTGGTGATCTAAGAACTGGGTCAACTGGTGAGTGTTGAAGAAGTCACGAATGACAATTTGAATAGGTTTGGAATTAATTAAGGATTTAATGGTCAATTCCTTCTTTTCCGCTTGTTCAATTACTTGACCCTCTTCGTTAATTTGTTGACCACGGTTCACTCCATCAGCAATTGTGAGCTTTTCCTTTAAGAAGCGCTCCATGCGGGTGATTCCACTTTCCAAACGGGAACTAATTAACTCGTTAATTAACTTGACGCGTTTGTTACCTAAGTGATCAATGTCATCGTACTTACCGATGGCCGAAGGGATGTTAATCACATAACTAATCGAGGCAATAAAGTCACTTAAAGTAATTGCCTTATTGAGATCGTTATCGTTGTGGATCCCCACCACTGGCACGGATACATCGAAGTTGTCGTTGTTCACATAAACATCAAGTGATTCATACAACAACGATTCAGGGGAAAGGTTAACAGCACTACCATCAGTGGTTAACTTAATCTTTTGGACAAAGTCAATTTGGTTGTTTTGGGCAGCTTGCTTAATCTTGTCGATTTCTTCTTTAACTAATAAGGTGCCCTTCTTTAATAAGAGTTCCCCGTTTTTTAAATGAAGGTCACAAGCAAGTGTTTTTTGGTAAATCCGCTCGGTAATTCTTAACTTACGTGATACCTTATAACGACCAGCAGCGGAGAGGTTATACCTTCTTTGACGGAAGAAACGTGAACACAGTAAAGCGTGGTAACTGTTTTCCGGACATTCATCGGTGTTTTCTAAACCACGTAGTGAAATGGACAATTCATGAACAATGTGTTTAGCAGCCCGTTCGGTGATGATTTTATCCATCAACTCATCCATGTCCGCTTCTAACTGACCTACTTTAGGGTCATTTGGTTTAGCTTCACTGAGCGCTTTGTGTTCCTTTTCTAAAAGGACATAGTCACTCACTAAGCTGCGGATCTTTTGGTCAGAGGCAATCCCCTTACGGCGGAGATCAGTTTTGCCATCGCGGAATTCCCTTAATAAGTTTTTAATTTCAGGGTCAACGTTGTTTAAGAAATCCTTAGCGTTGTAGACTTCCGCTTCTAAGGAACGGGTAATAAACTCGTTGTTTTTGAAAACTTTGAGGATTTCCTTGCCACTCATCCCAAAGGCTTTGAGCAAGGTGGTAATAGGGAAGATCTTTGCCCCTTCACGTACAGCATCCCGGAGCAAGATTTGCACAAAGGCATCTTCAATCTTCTTTTTATTGGAGATGTAGATCAGCATTACCGAACCGTTGGCTGGTAATACTTCACATACATAACCTTCTTGTACCCGTTTACGCGAACTAGACAATTTTAACTGGGACTTAGTGAGCATGTAGATCCCCGGTGAACGGGTAATTTGGGCAATCACAAACTTCTCAATCCCGTTGACAATAAAGACCCCATCGCGGGTAATCAATGGTAAGTTAGTTAAGAAAACGCCATCGACACTACTGGTGGCACTGTTCTTCTTGGATTTGCGCGGCTTTTTAATGGTTCCGGTAGCGGAATCAATTAATTCTAAGTCAGCGTAAATACCAATTTCATAGGTCTTGGACTCAGAGCGTGATTGGGCTTCATTACGTTCAGGAGCGGTACGACGCAAACCCTTAAAGTTGATCGTGTAACGATCATTAGGGGACTTAATTGGAAAGTAAGCAGCAATCAGGTTTTCCAGATCGTGATCTAGAAAAGTTTGATAGCTCCTAATCTGAATATCAGCCAGATTAGGTTGCACAAAATCAGTGGCAATTTTGCCATAGTAACGGCGGGTAGCAGTGGGTGAGTATTTTTTCTGAAAGAAACTAGGTTTTTGTGACATTCGTGTACAAAAAAGGGCTAAAAATAACAGTAAAAAGCCACTAACAGCGACAAAAGGGCCGCATGCTAATGGGCTTTGTAAAGTTTGCTACATTATATCAATTTTTGTACGTCAGTGATAAATGGCTGAAAATCAAAGCCATCATGATAGCCATTTACTAACACGGCTCCCTTAACGTTGAGACCACTAAAACTAAAGAATTTAGTGAGTAGTTCCAGCACTGTCTTACCACCACTCTTAGCTGCAGGAGTGACACAGCAAATAATTCCATCAAGCTCCTTTAAAAACTGGTTTTTACCGTATTGGGTATCGCGGGTCATCCAGTCGATTGTGTTTTTAGCAAAGGCAGGGACAAAACCGTTGTGTTCTGGAGTGACAAAAATTAGCGTTTTGTGATCGTGTAGTTTGCGCACTAAACTCTTAATTTTTTCGGGAAAATGATCCTTTTCCAAATCTTCACAATAAAAATCCACTTGGTAGTCAACTAGTTCAATTAATTCAGCATTGAGTTGTTGTTCCAAAGCTTTAGCAAACTTCCGGTTAATTGAATTTTTGGAGTTAGCTAACAATAAGATTAACGGTTTGGTACTCATTTTTTTATCTTTGATCCTATTTATATAAAAGAAAATTGTTAATTAAAGCGGAGAAAACTTAATTTAATTTTTAGTTTTCTTTTTTGCAACCAATTTCGCTTTAGCAACCCACTCCTCATTACTTTTCCTTAATTCTTGAGCTTTTCTTTCTTTGATCAACTTAATAAGTTCATTGATTTTTGCTTGTGCTTTTTCCTTATAAAACTCATCAGTAGCTCTTCTAACAGCTTGCAGTTATTCTCTTTTATCGTGATCAAAGGTGGTATATCATTCTGAGTCCAACTCTAATAAAGCGTCTTTTTTAGAAGCTAGTCGTTCTTTAGCTTCTTTGGCATTTCAATGATCTTCTGATGGTAGTTTGGTGTCCATTGAAGATTTAAGGTACTTTTTTAATTCTTTGAGATGCTTCTTATCTTCGTTCTCTGATCATTTTTTAATGCTTTTTAAATGCTTAATTAAATCTTTAGACTGATCGTCTTCTTTAAAGCTTCCTCCCTTTCTTAGACTTTTGAATAAACCTTCTATTCTATCCAATACCTCTTCTTTATGATCATCCTCTAAGTTTTCCTCTAAATTTTTCTTAAATAAGAATTGAAAACAATAAACATCTTTTTTTATTAGTTTCACAACTACCAAAACTTTCACAGCTAAATTTATCAGTGTCTCACTTTTTTAATTCTTCTTTCGTTTTTAATTCAATTTCTTGAATTCTTTTTTGTTTTGCTTGTTCTTCTTTAGCTGCACACCCTCGGAAAAACTTATTAATCTCATCTCTCTTTGAGTTTTCCATTTTTTTCAATATAAAACAAAAGAGCACTCCACTAATGTGAAACGCTCAATTGTTAGAGACAAATACTTATACCTCTAAATCTTTTGAACAGATTTCTATTAATCTATATGCTTGAGAAAGATATGGATAGGGTCTCAAGAAAAGAACATCTGAATAATAAAGCTTTGAAGTTTTAAACTTCTTAAACCCTATATTCATTTTTTTCAGAAACTCTTTCCAAATTCTTTTCTTAACCACTTTATTTTTAGTGCTTCAGATATCACAAATAAAGCTAATACGCTTTCAAAGTTAATGCTTTTAGTTTACTTTTCAATAAAGAAACAAGGCTCGTATTGCTTGATGTCACCTATTAGAGTATTAGTGCTTACGGTGGTTAAACCAACGTTTTGATCTTTATTGGCCTCTAATAAACAGATGCAATTATTGTCATAGTTGTAACCCTTCATTACTTCAACAATAAAATAGGCATCTTGTTTAATCACAATTGCATTAACTTGTAAAGTGCTAGCTTCTTGGGCAAACACTTGCAAGTTTTCAAAATACAGCAATAATTGTCCCGCAAAGTTAATAAAGGCTTGCTTTCAAATGGTATTGTTATCTATTTGTAAGTGGTGCTTAATACTCTCCAAGTTTTTGAGTAAATACTTGTGTGGTACGTTGAAGTGCACATCAATGTCTAAGTTATTTTCAGTATTAAAGCGTTCTTTGAAACTGGTAAAGCTCAGGTCAAAACTGTTACTGTTAGCCATAGAAGTGCCAAAAGCAAACTGTGAATTTAACTCGACAAAGAAAAGGTTATTCTTTGTTTTTTGCTTGCGCTTTTGGTGGTTCCAAGTATTAAAAAACTTCTTTTCAAAGTTTTTGCGGCAATAACGTTTGTACTGATAACCGTGCTTTTTAATGATTTTAACGATTTCATAAGAAAGCTTTGTTCGCTTATTTTCAAACGGAGAATTAACTAGTAAAACCACTAATTTCAGCCCGGAAAACTGTTTAACTGCTTCTAACAGCTTTTTAATTTCTGTTTCTGTTTGTTTGGTTTTTTCGACCAACATAAACACTGTTTCCGCTTCTTTATGTAACCTAATCGCTAAACCAAAACCACTTAATAAGTTATTGTCAAAACTTAAAAAGCGAAAGTGATCAAAGAAAACGTGTTGGTAAACTGTTTGACTCGCTGATGGTACTAATAAGTATTTAATCTGATTCGACTTAACTAGGTGATTGACAAAATCTAAGTATTCTAAAGCGGAAAGTAATCCCACTTTATTGGTGTTTTGAAAGCAATCGTAAGCTAAAAGTCTTTGCTCATTCACCAAGGTTTGCTCGTTAATGGTTGTTTGTGCCATACCTAGTAAATTTTACTTAGTTTATTATGCTTCAAAATATCGTGCATTGCTTTACAGAAACTTTCATGCTTGCTGTTAAACATTTGGTGTTGACTGTGTAAGATAACCCCTGATTGCAAGTTACCTTTCTTAATCAAAAAGCGGTTAAAGTAGTTTAAAGTAGCTTTTGTAGGCATAAAATCATCATGTTGAGCCAACAGTAAAAAGGCTGGTATGGTAATAGTTTTATAAGCACTGTTTAAGCGCGACAATTCCTTAAAGATCTGAAAGGTGAGTTTTTTGCACATAGCAGTATGCTTGTCTAACTCCATTTGTCACTGTCCATCGTTTTGTAAATCAGGAAACTTTGGTTGAAACATCCGCTTAAAGTAAGACAGCTTTTCGTCCTTCTTTTTAAAAGCCTTTTTAATCTTGTAGTAATTCAACAAGTTGGAATAAGACAGTGGCGCTAAGAGCACTAGTTTTTCCACTCGTTGTGGTTTTAAAAACTGCAATGCATAACTAGCCACTGCCCCACCCATGCTGTGTCCTATTAAAACAATGTTTTCTAAATCTTGCTTTTCAATTCAAGCTACCAATAAATGGGCATAGTGAATAGGTGACAATTCCTTGTCAGTAAAACCATGGTGGTTATGACCCGGTCACAAGGGTGCGTAATAGTCATAATCCACCAGCGTTTCAGAAAAGCTCTTAAAATAAGAATGGTGCACTGAAAAACCGTGCGCAAACACAATAGTTTGTTTAGCGGGTTTTTTAGCTGGTTGAAAAAATACTTCTAGTTGGCTAATATCAACAGTATTAATATTGACTTTGTCTCTCACAATTACCTTTTAAGAAAAGTAAAACACCTTGCACACAATTGGTGTCCTTTTTTAGGGTAAATTTGGGTTTGGAAGTTCCAACATCGTTCACACATGACTTGATCGGTGTGTTCAACCTTAATTTCGTTGGGGTCATTCGTTATATTTACCTGGTTTACGTTTAGCCAAACTGCTAAATGTTTTACAAGACGTTGATCAATTGCACTAGTAGAATCCACACCCAATGTCAATACAACCTGGTTGTTTTTAGTAATCACACCATTTTGACGTGCTTGTTCAATGGCTGCATAAGCCCGGTCTTTAATGGCGCTAAAGCTTTCGTGTAAGCGATCTAACCCTTTAACATTAGCAACTTTAAACATGGCTGGTCCTGCAAACAGGTTTACACTAACCGGCTTTTTAGGCAAAAGATAATTCTGTCAAGCATCTTCGGCAGTATGGGGAATAAAGATATTTAAGAAACCAATTAACTGGGTAAAGATATAGTTCAATACTGCCTGCTTAGCCAACCGATTAGGATTAGTTTTAGCATCACAATAAAGTGTGTCCTTAATAATTTCAAAATACCAACCCGATAACCACAACACAAACTTATTAATGGCCTTTAAGCAATTTAAAAAGTTGTATTGCTTTAACCACTGGTGCAATTCTTGTACCAATGCATTAGTTTTATGGATTACGATTTTGTCCTCTAGAGCAAAACGATAATCCTTCATCTCACCAAAGTTAAAGTGGTTGAGGTTACCCAATATAAAGCGCAATAAGCTGTTGCGAATGCGGCGGTATTGCTCGACAATTTGCTTGATAATGTTGTCACCTATGCGGTTGTCCACTTGTCAATCAACATTAGTTACCCATAAACGCAGAATGTCTGCGCCATACTGATCACACAACTTTAAAGGGTCGACCACATTACCGAGTGACTTGGACATCTTATTACCATGTTCATCCAAGGTAAAACCATGGGAAATCAAACTCTTGAAGGGTAATTGGTCAGTTTGGATAATACCGCAACTGGCGGATGAGTTAAACCAACCGCGGTATTGGTCACTACCCTCCAAATAAAGATCAGCGGGAAAGTTCAGTTTATTACTAATTAAAACGTTGTAAGTTGAACCAGAGTCAAACCATACTTCCAAGGTATCAGTTTCCTTTTTATATTCAACTCCTGGCTGTATTTTCTTTGGGTTTAAAAAAAAATTAATATCCTTTTCAAACCAACTGTCAATCCCGTACTTTTTGAGCTGTTGAATGGTGTATTTAATGGTAGTCACATCCAGTAAGGGTTGGTTATCTGCATAAATTAAGGGAATCGGCAAGCCTCATACTCGTTGTCTGGAAATACACCATTCAGCACGTTGCAACAGCATTTCTTGTAGGCGCTCTTTATTCTTGTTATTAACAAACTTAACCCTTTTAATTTGACGCTTTAGCTTACTTTGAATTGATTTAGTTTTAATGAAGAGCTGTTTGGAGGCACGGTAAATCACCGGAGTCTTACTGCGTCAATCGTGGGGTTCGCGGTGCAAAAAGCTTTCACTAAAGACAAAGTTATGGTGTTGCTTTAAGCGTTCAATAATCACATCATTAGCCTTTAGATAAAACAAACCCGTTAAAACTGGATCGTTCAACGTATCATTAAAAACTCCTTTTTCATCAAGTGAAACTAGCGCTTCATTTAACTTGTTTTGCTTGCACAAATAAAAGTCATCCAAACCATAAGCTGGTGAAGTATGCACGAGTCCTGTACCCTCATTACATAAAACGTGATTACCCAACAACACCGGGTTCACTTTGTCATAAAGACAGTGTTTATAAGTAGTGTTTTGTAATTGGCTACCACTAATGGTTTGTACTTGTATAGCATCAGTTCAACCAAACTTAGTCTTAAGCGATTCAAATAAACTGGCCAACACCACGTACTGCTCGTTATTATAAGTAAACAGCAGGTATTCAAACTGGGGGTGAACGGCAATTGCTTGGTTAGTAGGCAGCGTTCAAGGAGTAGTGGTTCACACTAAGAGTTTAGTACCTGAATTTAATACTGCACTCTGCACTACTGTAAAAGTAAGGTAAAGTCCAATTGTTTTTACTTCTTTGTATTCAATTTCGGCCTCAGCCAGAGAAGTGCGTGATACAGGCGATCAGTAAGTTGGTTTTAACGCTTGGAAGATTAAATCCTTTTTCACTGCTTGTAAAAACAGGTCTAGCTCATTTTGTTGAAAGCTTTCATCAATTGTTTTGTAATACTTACTAAAGTCATTCAACAGACCTAAGCGTTGAAACTGGGCTTTTTGAATGGCAATTTGTGACAGGGCAAATTGCTTGCATAAATCGCGCTTTTCACTCAATGATAAGCTCGCATAGTGTTGCGGATCTTTTTTGCTCACCGCATGCTCTATGGGCAAACCATGGCAATCCCAACCAGGAATAAACACTGTACCAAAGCCTTGTAAGTTTCAACTACGGAGCACAAAGTCCTTCAAAATTTTGTTTAAAGCATGGCCAACATGGATATTGCCATTAGCATAAGGCGGACCGTCGTGTAGGATTTTTTGTGGTTTATCTTTGTTTTGGCGGTGCAGTTTTTGGTAAATCCGTTTACTTTGTCAAAAAGCTTGAAACTGCTGTTCCTTAGTGGTTAATTTGCCCTGCATTTCAAATGCAGTTTGGGGCATTAACAGTGTCTTTTTTAAGTTCATTAAGCATAGTAATTTTATTAATATTTAACTAACTTCTATCTGATGTATAAATCAGCGATTAATATCGTCTTATTTTGCCCAGAAATTCCTAATAACACTGGGAACATTGTTCGGAGCTGTACCGCTTTTAAAGCTAACTTGCATCTGATTAAGCCCTATGGCTTTTTTCTCAATGATAAGCGGATGGTGCGTGCTGGGTTAAATTGTTGGGATAAGGTGCAAATGTTTGAACACAAATCCTGGGAACACTTCATTGAAACAACACCACCAAATAAAGCAATTTGGTTGTTGACAAAGAGTGGTACTACTACCCCAGACCAAATTAACATGCAAACAGATAACAGGGAACAACTGTACTTTGTTTTTGGTCAAGAAACCAAGGGATTACCGCAAACACTCATGGAACAATACTCGCAGAACCAGGTACGGATTCCGATTTGAAACCCTGTAAGAAGCATTAACTTGTCCAATACAGTAGCTTGTGTACTGTATGAGTATGCTAAACAAAATCATTACTTTAATTTAGATAAACAGTGCGCTTAAGAAAAGTTAAAGATGCTCTTGTAAGGGTACAAGACAGCCCTTACTTTAGTAACCCAGAACAACTGTCAACTATTGAAAGTGAATCGCTCTTCGTGGAAATAGGTTGTGGTAAAAGTTGGTTTTTAAGCCAGCAAGCACAGCAAAACCCAAAGTGTTTTTTTGTGGGAATCGAGCGTGAACCCACGATTGTTTTAAAGGCAATTAATAAGGTAAACCGCTTAGAGGTTAAACCACGTAATTTATTAATAACGTGTTTGGATGCCAACCAGCTAACAGAGTACTTAAAACCACAATCGATTAGCAAAATCTTCATTAACTTCCCCGATCCTTGACCAAAAAAGCGTCACACGTTGAGGCGACTAACTGCTCCACATTTCCTCAAGCAGTTTCACCAACTTTTAAAAAAGCAAGGTTTAATAGAATTCAAAACGGACAACGATCAGTTGTTTGAATTTACCTTAGAAGTCTTGCAGAAAACAGTAGCTCACTTTAAGATAATTGAGCAAACGACAGATCTGCACAATTCACCTTTGACTAGTACCAATATTATGACCGAATACGAACAGCGCTTTGTTAGTTTAGGGGTTAAGATTAAAAAGCTAACGCTTGAAAAACTAAATTAGTAAAAATTAGAATAATTACTGCCTTAATGGCGATTGTGGCGAAGTGGTTAACGCACCTGATTGTGGATCAGGCATTCGTGGGTTCAATTCCCATCAGTCGCCCCATTAAACATACAAGCAGCAGATTAAACTGCTGCTTTTTTTATTTAGATAACTAGTTTAAGGGTGTGTAAACACTATCCAAGAAATTGAGAAAGTTAATGCTTACCTGCACGGCATGGCGTTTAACGCCAAATGATCCAGCTGAATAGGATTTTTTAGCGGCGGCTGCAGCAGGAGCTGCTGGAGTTGTTGAACCGTTATTTCCACTGTCCTGATTTGGTTGGTTTGAACCTGGTGCATTACTGCCGTTACTGTTATCAGGATTATAGAAATGCGCACTAATTTGATCTAAATTGTTTTTGCGAAAAGCAATCACAATTGCTTTAGTTACATCAGTGCCAACCGTTTGGGCATGAACATTGGACTGGTTAATAAGATTATTGACACTACCGCTGTAAACTGTTTGAATAAAAGTCACAATTTCGCGGTATTGAATGGCAGATTCGTCGTTTCTTCTTAATTTGTCGGAATTGTCCTTATCAGTTTGCTTAGCTAATTCGTTATCACTAGCGATAACTGGATAACGTGCAAACGGATTCCACTTAACAGGGTTAGGATCAATGTCATTCCAAATTAAAAACTTAACTCCTTTAGGGTAAGTTGAATAACGGTTTTGAACCTGCTTAACGGTAGTACCTAGATCACCAGCTCAATTTTCGGTACTAACAGCTTGATTGTTTGAACCATTTAGAAAGTTAATTTGAGAACTGTCAGTTTCAGTGGTTACTACAAGAACGTAGTTACCATCATTGTATTCCGCTGTTCCAAAAACTAAGTTTCTAACCGTTTTATTTTCTTTAGGTAGCTTACTGTAATCAACTTTGTTTTCTACGCTTTGCTGTGAAAGAGTGCGAAAAACATTTGGTTTATCAATATTGCTACAGGAACTCAAAACAATTCCTGAACCTAACAAGAGTAAGGTTGAAAAAGATACTTTCTTTTTAGAAATTGAACTAAACCACTTCTTCATTCAAATACTAGTATTGGTGTCGGAGACCAGACTTGAACTGGCACAGTCTTGCGACCACAAGCCCCTCAAGCTTGCGTGTCTACCATTCCACCACTCCGACGCTGGCGTCTTATAAATTATTAATAGATTTTAAAAATCCATAAACTCTTGATAAGGAAATTTTGCATTTTCTCAACTTTGTGCTTTGTAAATTAATCTATAAAACACAATCTTTTCATCACTTTGACGCTTTTTAAAACCCAACTCAATTTATTTAGTGGGGGCAGGAAAACGAAAATTTTGTGTTGAAGGCCTGATTTTCTGTTCCCAAAAAATGCGTATTTTATTTTTTCATTGTTTTCTAAATTACTTATCGTAAAGGCTTTCAGCACAATTGATGTATCTCTTTTTTTAAAAGAATAAAGGTCATATATTCCTTTCATTCAAATCACTAAAAGTTACAACTTGCTGTTAATTTCAAAATTGTCAAAAAGCCGTCTGTTTTTTTCACCTTGGGCAGATGGTTTTTTTATTTGTGTGAATTAAATAAAAATACCAACAGCACCACATCTTTAGGTTTCACTGTTGGTTTTTTATTTCTGAGCCTTGATTATTTGGACTCAAGTTTGTCTATTCTTTTTTCCATTTTGTCCATTTTTACTTCCATCTTGTCCATTCTATTTTCTATTTTGTCTATTTTTCCTTCCATCTTGTCCATTTTTTCTTCCATCTTGTCCATTTTTACTTCCATCTTGTCCAAGCGATCGCTCATCTTTTGCAGCGTTTGCAAGATTAGCTTTAAGGTTTCGCCCTGAGCTTTTTGCTCGATTTGAAGTTCTTTAATTTGCTCAACCTGGATTCTTATTTGCTCACCTTGGGCCGCCAAAGTGGTTTCAATCTTTATGAGTCGCTGATTGTTAGAATCTTTATATTCCTGAAATTGTTCTTGGGTAACGTATTTAACTTTCACAGGTGGTTTAAGTTTATATTTTCGCAATTCATAAGTTCAAACTCCAACTGTGCCATCACCGTACTCTTCTTTTTCACCATCAAAGTGTTCTTTTCCAATTTTACGGATTAAGGCTTTGTATTCCTTTTCGGAAATTTTTTCTTTCATTAAGTAATACCTCCTTTCTTTTTACAGAGGTTGAGAAAACTACTACTTGCAGCAGTGCTGCCAACTAAGTTCAGCTGTTTTGCTGATTTAATCAATTTGTTTATTAAAGGCATTTTTTGTCCTCCACTAAATAAATTGAGTAGGTTATTTAGAAGTGTCAAGAAAACAACAAATTTTGAAATAGACTAAATACCTTTGCTAATTCTTAACAGTAAGAGAAAAGAAGCCTTGTTTAGTTACTAGCTATCTATTGCCATTAGGACAACAGAAACAGTTTGCTTGCATTTAAAGGATTTAAATCTCTAATAAATTACTTATCAATGACACTGAAAAACATAGCTTTTTTGTTTTTATTACCCTTACTTTTTCCAAAAATTCCACCTTTTTTCTACCTCAATTTTCAAACCATCTGAAAAATAAAAAGTTATTAACAAAATGTTGATAAAGACCTAATTTAGTGCATTTTTGTGCCTTTTTAGTGTTAATAACTTGTTTAAAACTGCTTCTAAGTTCTTTTTGGTAACTATCAGACTAAGCCAGATTAGCAAAAAATGTTTACGAGCCTTATACTGATTAGCGTCTTTGCAGTTTTATGCAGCCGAACTATTACCGTGTCATCAAGAAAGCAACCTCATTTTCAGGGTTAGATATGATCTCTCATGCCTATGGTCAGATTGCTGGCGTTGAAGTTGTGGGTTTTTATTTATGACTAATTACTGAAGCCAATGCTCAAGCCTTTAACAGTGAAATTAGAACGCCTATTTCACGTCTACAAAACGCTTTCAATACTACTGGTACCAAAAACGTTAATGTACCTGATTGAATCTATAAAACCATTGGTAAATTAGAAAGCTTAGGTTTGGTGCGTACCTTCTTTTCACAGGAAAAATCAGAAATGACTTTTTGAATAGTCGAACCATTAGGTTGAAAAGAGTTTAACCAAAAGAAGCACTTTAAAGAAAAGCTAATTGAATCAATGGGAAAACTGGAATATGACCGCAACTGCCTTTCCTTTGAACAAATCGATAACATTCAGTTTGACAACGCACTGGAAATTACCGCTAACTTTGAAGCTAACTTTACCAGTAAGCAAGATTGTCTTAGCTTTAGTTTTGACTTTGAAGCTTTTCATAAACAATTAGTTAAACAAAATCTCTTTGTTAGTTTTAACCAGAAAACTAAGGCTGTTATTAATGGTTACTTTGAAAAATACCAAATAACACTAGAAGGCATATTGGAATGTGTTATCCCTAGTGTAGTTAATGATGAAGTTGACTTGGAACTGCTACAAAAATTACTAGAGCAGCTAGTTAAAAACAACACAGCTCCAATTGTTGATACTGTAATAAATGACCGCAACTTCTTTTATGATAATCAGAATTTAGCCACAGAAACAAAAGATGCAATTTCCCGTTGTCATTTGGAATACAACGCGGAAAAATACCTATTTCTCTTGTACGGTAAAGTAGAAAACGAGCAGTTAGATCTAATTCGCCGCTTAAGACAAGAGTTTGGAATAGCTGATAAGGTAATTAATCTAATAGTAGACTTCTCCTTTTGGAAAAACAACAGCATGTGACGCGAACAATACATCTTAAAGATTGCTGAATCAGTAGAGCGTTACCGTAGTCATAATAATTACCAAGCCACCCTTGATAACTTTATCCGTGCTTCTAGTTTAGCCAAAAAGCAACGTACCAAAACTAAGATAGAAAAATCCGAGCCTGAAACTAGTGCTGCACCAGTAAATGAAACTGATGACGTCAATTACTTTTTAAACCGTATTAAAGCAATCAACAAGAAGAACAGGGAAAATGGAAAACACAAACGCTAAAACTCAACCAGCAATAAATGAGTTAGATAAGACTACAGTTCAAGACACAATTAAGAATTACGAACAACAACTTCAAAAAGAGCTTAATGAAGAACAACTAAAAGCTTTTAAAAAGCGCTTTAAGTATTTGAAGCTAACTAAAAGAGAAGTGATTGAAAAGGAAAGCAGGGAACTTTTATTGCAAGCATTTAGAGACTTTGAAAATTGTGAAAAGATAAAAGGCAATCATTGTCCTAATAAAGGCAATGCTCACCTCAACATCTTTCGCGAGCCACCTTTTTGAACTAAAAGTAATAAAAAAGTACTGGGTAAGTTAATTGGTATAAAAATGTCCTGCGCTAAATCTATTGATAAGGAGGATATCTTTAAATTGCGCTATAGTAATTTCATCATCTATAAGCCTAACTGATTTAAATACGCTGACGAATTTCCACAAATTAACCAATTACCCATTAACAAATTTCAAGAAGAATCTTTCGAAGAAATAAGAAATGATCTCAGTGAGCTTTATGAAAATGAAAAAAATCACAAGGAAAACATAAGTTTCTTTAACTTACAAAAAGTTAATTTCAATCACGAGGGCGCTAAATTACTACTTTGTGATTTTGTCGAAAGAGGAGTTAAAACTGCGTTGCTTTATTGTGAAGAATTTGTCAGTCGTTTTGACCGTGCATACTGAAAAGTAGATGATTATTTAGATTTAATCAATGAAGCTAATGTCATCATATTTGTCAGTCTTGGTGAAGAGAGTTTTGTTTCCAAAAACTACATTCTCTTCTTAACCAGATTATTCGACATCATTAATTCAAAACGCAAAGATGTTTATTTCTTTAGCACCGAATACAATGAGAAAAATGGTCTCATTCAAGCATTTCAAAAAAGCATTCACTTTAAAGCTAAATGAGTAAATTCCTTTTTTGAACAACTCAATTATTTTTTTGAAATGAAAAGAGAAGAAATTAAATAAAAAGCGTACTAATTTATTTAGTAACGCAAAATTATTTAAAATTAATCGTAATTATTTCCCCTGGAGTGGCGGAATGGTAGACGCGGTGGACTCAAAACCCACTAGGAAACTGTAGGAGTTCAAGTCTCCTCTCCAGGACCATAATTAAATACTTAAATTTACACTACGAAATGACAACCTCAACGACCGTTGGGGTTGTTTTTTTATTTATTAAAGGTAGTTTAAGTAACGAACAAGATTCCTGCTACTAATAATTAATAGACAATCTGCGCTTTCTTTCAGTAAAACTTTTGGAAAGCACGCATCACTGGAACACCAATTAAACTCACTAGTGTGAATTTAATCACATTAAAGAGTGAGTATAAAGCAAAGATGCCACCCCAATAATTAGGTACGCCAAATAAAAAGAAGTGAAAATATGGTGTTTTGTTATATATCTTGGCCACTTCGACAAAGTTAGCAGTCGGCACATAGCCAAACCAATACCAGTATAAAGGGGTAATTAACAAACCGTTGAGCGTTGTTAACAGCAAAGTGTTAGATAGAGTAGCCAAACTAAATACCCATAACCAGCGAAGTTTAATTTGAGCAAAAATGTGAAAGTACAACCGTGTAAAACTCACGATAGCAAAGTTAGCAATGGTTAGGGTTAAAATACCAATCCAGTTATTACCATCCCAAATAAAGCTACAAAGTGACAGCGCAACGGTAATAACTAAGGCTCAAGAATAAGAAACAAAAAAAGCACAAGCAATTAAAAAGACTGATGAAATGTCAATCGTTAATTTAAGTGCTGACAAAAACGGAATACTGATAAAGTGACTAACAAACGAAGCAATTAGTGCAAGCGCTAGCATCATGGCACTAAAACTAAGGTTAAACAGTTGGTGTTGTTCTTTTTTTGGCCAAAAAGCTATTCTTGCACCATTCATTATTTAGTAAAGCCTACTAGCTAGAAAAGTAGTTAAATAATAATATTTATTAATATGGACAAATTTTTAATCGATGTTACTGTAGAAATTCCTAAGAGCAGTAAGATCAAGTATGAATACGACCGCAAGACTAGTCAAATCCGTGTCGACAGGATTCTCTTTGGTAGTGAATCTTATCCACAAAACTATGGTTTTATCGCTAACACCTTAGACTGGGATGGTGATGAATTGGACTGTTTTATCTTCGCTGACCAAGCCTTTCTACCAGGTGTAGTAGTACCTACTAGAATTGTAGGAGCTTTAGAAATGGTAGATGACGGTGAATTGGATACCAAACTTTTAGGGGTCATTGATTGTGATCCGCGTTACAAAGAGATTAACTCCGTCAATGATTTACCAAAACACCGCGTTGATGAAATTATTGGTTTTCTCAAAACTTACAAATTACTACAAAAGAAAGAAGTAATTATTAAGGGAGTGCAAAGTTTGGAGTGGGCTAAGAAAGAATACCAAGTGTGTGTTGATTTAATGAAGCAATATGGTAAGTTGCCTAAGGATGAGTTTATCGCTAAAATGCAAAAACTCCATCCTGAACACTACCAAAAATAGTTCAAGTTATGATTAGCGCTAACCGTGGCATGTTTTTGGAAACAATTGTTAACCAAACAATTGTACGACTAGGAGATCATCCAGATATTTGACTCGAAAAGCGTTATCTACCGATTCAACCAACTGCTTTTAAAGAAACCCGTGTGCAAGGAGCATTAACAGCGCAAAAGAAATCGAAAACTGACTATTAAGGTGTTTATAAAGGCGAAATTTACTTTGACTTTGAAACTAAACAAACTAATAAAAGTAATTTTGCTCTAGCGCAGTTAGCAGAACACCAACTCAAACACCTTAAAAGAATTAATGAGATTGGTGGTATTAGCTTTTTGTTAATCTATTTTCAGGTACAAGACCTCTTATATGCGTTGCATACCAAGGATCTCTTGCAGATGATCGAAGCGCAAAAGGGTAAAAGTAAAACAATTAAGCGTGACCTTATTGATCAAAAAGCACAAAAGATAAATCTAATCTACCCAGGGGTTATAGATCTAATTGATGTAATAGAAAACTTTATAAAAGACTCTTAGTCTGCGTATTTTCAGCGTACCTTAGCAGCCGTTTCCTTAATGGTTTTGCTTGGACTGTAACGCACTAATGGTTTTGGTGGAATGTGTTTCATTTCACCAGTTTTCATGTCTTTTTGAATTCTAGCGTTACGAATCGTAATGCGTAATTTACCCAAATTTTCGGGCAAAATGCAAACGCTACGACTTACTAATTCATTAAGTAAGAGGGTGTTGAGGTATTTAAAGATCTCTTTTATCTTAGCCTCTTTTACTCCAGTAGCTACTGCAATAATCTTGTTAATTTCGCTACGAGACAATGGCTTACTCGATGTTGTTGTTTTTTCCATTAAGTTTTAAGTTCAAAGTTTGAAAATCCAATTTGATTATAAAAACGGAGGAGAATCAAATTAAACGCGTTTTATTTTATTATTGTTCCAATCTTTAAAAGGTTGCACTTTTAAAGGTTAAATTTGACAAAAAGTTTTCCTTATAAGTATGGAACCAAATAATTTAAAAGAAGAGCTAGTTTCAGTCTTTGAAAAAGCTTGTTCTTCACATAAAGAAAGGCTTGATTTTATCTGTTCTGTAAGAGAAAGCGATACCTTTTCCAATGTGGATGTACCGCTAGCTCCTATTAAAACTATTATCGAAATTGCTAAAAACGAGGAAAATCAAACCGAAATTCTCAAATTAGCGATCGAAAACATTAAGACTCTATCAACTGTTGGGTCTGGTCAATACATTGCATCGCACTTCTCAACCCATAACGAAGTTGCTATTATTTTCTGTATCTCTTACTTTTTGTATCACTTCAACTTCTTGCACGATGAAAACAAAAAGCAGTTGCTAAAACGTGCCTTTGAAGCAGTTGCTGAAAAAATAGCTGACTACTTAAACGAAAACTAAGATTTAACCTTATTGGCTACTATTGCCACTTTATCTTTCTCAACTATTACGTTGTAACTAACATCTTTAGTAATAATACCTTCCAGAATTTGCTTGGCAATTAGAGTAGCTATTTGCCGATCGATAAAACGTTTAATTGGTCGAGCACCAAACTGTTCGTCGAAACTAGATTTGTAAATAAACTCATTTAACTTAGGGTCAAACGTGAACTTTAAGTTTTGTTTAGCTAATCTTTGATTTAATTGATCCAATAAATTTGTAATAATTTCTAAAACGGTTGTTTTTTGCAATACGCTAAAGAAAACGATTTCATCAATCCGGTTAATAAATTCAGCACGGAAGTGTTTTTTTAGTGCTTCCATCGCTAAATCGCGTTTGTTTTCCATAATGTAATTGGAACCCAAGTTGGAAGTCATAATTATCATCGTATTCTTAAAGTTAACTAAACGACCTTGAGAGTCCTTTAAAGTACCATCATCTAATACTTGTAAAAGAATATTAGTTACATCAGGGTGTGCTTTTTCAATTTCATCGAACAGAAGCACACAGTAAGGTTTGCGTCGGACTGCTTCAGTTAGTAAACCAGATTGTTCATAACCTACATATCCAGGAGGCGCTCCGATTAACTTAGCGACCGAATGCTTTTCCATGTATTCACTCATGTCAAAGCGAATCATCGCTTTTTCATTATCAAACAAGACCTCTGCTAGTGATTTAGCCAATTCCGTTTTACCAACACCAGTAGAGCCTAAAAAGATAAAGGAACCAATTGGCCGGTTAGGGTCATTGAGGTTTACCCTTCCACGCATAACGGTATTGACCACTGTCTCCACGGCCGCATCTTGACCCTTAACCCGTTTTTTTATTTCATCACCTAAATGCAAAAGCTTTTCCTTTTCGGTTTCTAACAGCTTCTTTAAGGGAATACCGGTAGCTTGTGATACTACTTCGGCCACTTCGTTTTCTGACACTTCTGTCTTAAACAAGTCATGTTTGGAATCAACATACTTTTTCTGTGCTTGTTCTAATTGGTTTTTAAGTTTGGGGATGTCATAGTAAAGAATCTTAGAAGCAGCTTCATAGTTACCTTCCGTTTGGTAGAGCTCTAAGCGTGTTTGCAAATCCTCAATTTCCTTTTTGAGCTTGTTAATGCTTTCAAAGTTAGTTTTCTCCTTTTTTCATTCGGCAGTTAGAGAATCACGCTTTTTCTTTAACTCATCTAACTGTTGTTTTAGATTGTCTAAGTGTCCTTGTTTTTTCTTGTTATCAGCATTTTCCTTATCTTGTTTTAAAGCGGCATATTCAGTTTCTAAGTTAATAATTTCCCGCTTAAGGGTGTCAATTACCACTGGCTCTGAAGCCATTTCCGTTTTAATCTTGGCACTAGCTTCATCAATTAAATCAATCGCTTTATCGGGTAAATTACGATCAGGAATATAACGTGCTGACATTTCTACAGCAGCTACCAAGGCACTGTCAAAGATGGTGAGGTTATGAAAAAGTTCCCAACGGGTCTTCAAACCCCGCATAATGGTAAGTGCTTCTTGTTGACTCGGTTCACTGACAAGGATCTTCTGGAAGCGCCGTTCTAAAGCGCCGTCCTTCTCCACATATTCACGGTACTCTTTCAGTGTCGTGGCACCAATAACCTTAATTTCACCGCGTGCTAACATTGGTTTTAAGATGTTGGCAATGTCCATGGCACCACTACTGGAGTTACGACCTAGCCCCACTATTTGGTGGATTTCATCAATGAATAAAATAATCTTGCCATTTGATTCTTTGACTTGCTTTAAGATACCGTTAACCCGTTTTTCAAACTCACCTTGGTATTGGGTGCCAGCAATTAAACCAGACAACGAAAGTTCATAAATTTCAACATCACGCAAGTTTAAAGGCACGTCATTATTAACTACCCTTCTTACAAATCCTTCAACAATGGCGGTTTTCCCGACGCCTGGTTCACCAATTAAAACGGGATTGTTCTTGTTTTTCCGGCTTAAAATTTCAATTAAGCGTCGAATTTCGTTGTCGCGACCAATAATAGGATCAACCTTATTTTTGAGCACCTCATCGTTAATGCTACGCCCCATTTCCTTTAAGAAATCACGCTTATCTGGTGTTGGTGTAAAACTAAAATCCATCTATTTTATCCCCTTTCTTTGTTAATAGGCCAATTATATAAAATTAGCACTCGAATGGCTTGAGTGCTAACGAGTGGGCTAAAGATTTACCTTTTTTGGTGGTTATAAAGTAAAAGAGAGCCAACCCCCAAGTGCTGCTCTCTTTCATTTATAAAGACGGTTTTGAGTTACTTAACTAGTACAACGGAATTGTTTTGGCAATCTTCCAAGTGTTAGGGAACAGTGCCTTTAAAAAGTAGTTCAAGCGCTTTTGCACCCACTTTTTGTAATCAATAATTTGCTGTGTTTGAGGCATAATTTCAGTTCACTGAAAGGCAAACAGCAACACGTAAATTACCGTTTCAAAAACTAGTTTTTTGTCTAAGCTACCGTACAAGTCAATAATTTGGTTGGCTGTTTCAATTGTCAACTCTTCGCGAATAATGTTGGCAATCTCATAGTAAGGGTTGTCCACACAAGACCACTCAAAGTCAATTAAAACGACCTGCTTTTTTGGGGTATAAACCAAATTATCAAAGGTGGAATCGTGGTGACACAAGGTTTTGGGAATCGCTTTGTGTTTATCTGTTAATTCCACATACAGTTTTTTGTAAAAGTCAGGTAGCTTGGTTTGGTTAAAGTAAATTAACGGATCGAAGATTCAAATTTCATTAGCAAACTGTTTTCACTCGACTGCATGTACTTTTTTTAAAGAAGCCACTAGTTGTTCTAAAAACAGCCTTGTGGGACGTTTTGGTTGTTTTCCTTCAATCCACTTCTTAATGGCATTACCGGCAGTTACATCAAAGTAAACAAAGGGGTTACCAAAGATGGGGGTCACTAAGTCCAGGATAATTTGCTCGTTGCGTCGGTTCAACGGTACATCTTTACGCGCTAACCTGACTTGGTACTTACTCCCATCTTTCAGTTCTGTAAAAAAGGAAAAATTAGTAAAACCCGAGTGGATTTGTTCCACATGAGCTACAGCAGTTGGTTGGAGGTTTAACTTTTCACAAAGAAAAGCAATTGCATCGCCCTTATTCATCTATTAACTATTTTTGAGTTAGTCGAATGGAGTCTTCCACAATCATTAACTCTTCATTAGTACGTACCCGGTAAATCTTGTACTTACTTTGGGGCGTGGATACCACACAGCTATCACCGTACTTGACAAACAGTTTTAGATCAGTCGCAAAGCCTAATGATTTAACGGCGTTAATAAACAACTCTACACAATCACTAGCATTCTCGCCAATGCCACCAGTAAACACTAAACCATCAATGTTGCCTTCCAATTGGTTCAAGTATTTAGCAATGTAATCCGCTACCCGGTTAACATACATTTTGATAGCCACAGCGTTTTCTTGGGGTTTAGCGAAAACATCACGCATATCCGATGCACCAGTTAACGCCAACATCCCTGATTTTTTATTAAGCGCATTGACCACATCACTAGCACTCATGTTTTCCTGTTCTGCTACATAACCAATAATGGCAGGGTCAAGGTCACCACTACGGGTTCCCATAATAAGACCTTCTAGCGGGGTGAATCCCATTGAAGTATTAAATGACTTACCATTCTTAATGGCACAAACACTCGCACCGTTACCCAAGTGACACACAATCAGGTTCAGGTTTTGCTTGTTCAAGTGCTTTTCTAAATAGTTATTAATGAACTTGTAAGAAGTACCATGGAAGCCATAACGACGTACTAAGTGTTTTTGCTCCCATGAACGTGGTACCGCATACAAGTAGTTTTCTTGGGGAATGGTAGTGTGAAAGGTTGTGTCAAAGACTGCCACATTCTTAGCACTAGGAACTTCTTTGAAAAAGATTTCAATCACATCAGCCTCTGGCTTGTTGTGCAGTGGCGCTAACTTAATAAATTCCTTAATTTTAGCGAGTGCTTCTGCATCTACGATCACCGAATCCTTAAAGTAGTTAGCGCCCTGCACCACGCGGTGACCAACTAGAATAATGTCACTAAGTTCCTGGATAATTTTGTGCTTTTTAAGTGTGTTTAAAAAGTGGGTTACAGCGGCGTGGTGGTCGGGGAAAGCTACTTTTTCTTCAACCTTCTGTTCGTTAAATTCAAGTTTGAAAAAACCATCGACAAAAATTCGTTCACAAAGGGCTTTAGCTAGGACTTTTTTGTGATAATCAAACAATTGGAATTTGATCGAGCTACTGCCAGCATTGACTACTAAAATTTTGTTGTCGTTCATTTTTATCTAATAGGTAACAATAATAAAATATTAGTAATAAGTCCTCATTTTTTCTCTAATAACTGTCAATGAGTGCTAAAACTTCATATATTGTGGCTGCTGTTTCGACAGCTGTAGGTATTATTCTCTACTTTATCTCTTCCCTCATGGGGGTAATTGGTGTAAGTAATGATAAGACCTTAATCATTGTCTTTTTTGTACTAGCTTCAATCATCATTATTAACGCCTGTGTTTTTTGAATTATTTACCTGTTCAAAAAAAGAAACGTAGTCAAATAAAAAAGTTTGGCGCTTTAAATTCTCCTTACACATTCATTTAGATAGGAGCGAATAAATGTGTAATGAATACTTTTAAAGAAACCCTCTTTTCATTAAAGAAAAGTAATTATTTGTTGAATGAGAGTGCTCAAATTCTCAATGATTGTGTAGTAAATAACAATGAAATCGATGAACGCTTACGCAATTCGATTAAATTTGCCCTTGGCATTATTAATTTAGCTAGTTACTGTCTTAAAAACGAACAGGAACAGCTAGACCATGAACTCAACCATGGTAATGAAGCGCCCTGGGAGTTTAGTTTCAATGAAGCTGAACAACTAATAGAATGCACTGTCAAAAATAACTTTGGCAACGAAAAACTAGTGGATTTAATTTTCCACATTGGTGATGCCATGGAGACTTACCGTACCACTAATATTAAGTTTAGAGTACCCAAGTCTTACTATGATGCCAAACAGCAAATTAGAAAGGTAATTAAAAACTAAGGACTATGATTGCTTCAATTTTTGGAAAAATTACCTTTGTTGGTAAGCGAAAAATTATTGTTGAAGCCAATTGTATTTCCTATTGGTTTAATGTGAAAGAAAACCATAGCTTCGAAAAGAACTTGGAAAAACCGCGCCAGGTCTTCTGTCAGATCATTAAAAGAATGGTTACGAACCAGATCTTGGAAGAGGGCTTTGCCTTCAATACCTTAGAGGAAAAGGAATGGTTTTCGAAGTTCATCGAACTCAATGGCATTGGTAGTAAAACTGCTTTAAACTTGCTCAATAACAACCTCGAGGAAATGCGTGATTATATTAAAAACAGTAACTACCATGCCTTGACTAAGTTAACTGGTTCTAACAGTAAGGTAGCTCGCGCATTGCTGGCACTGGAACTGTATGACCGAGACGATGGTGGTAAACGGATTAAACCCAACACTGCCATGGCCAATGACTATGATGAGATGTTTGACACCTTAAAGTCGTTGGGCTATAAACCCCAAGATATCCAAAACGCTTTGAGCAAAATTGAAATTAAACCTAACTTTGATGTAAGTGAAGTGATTGCTGAAGTGATTAAGTTAATGTCTTTCCAGAACAATGAAGTTACAAATAAAACCGCCTAACAATTTCGCTGAGTTTGTCGGTAAACAAGAAATTATTAACCAAATTCAACTCAGCATTAAAGCGTCCCGTATCAATAAGGCGCAGTTGGACCACATTCTTTTATACGGTCCACCTGGTGTTGGTAAAACCACATTAGCTCGCTTAATTGCGAGTGAAATGAACACCAAACTGCAAATTATTCAGGGTGGGCACTTACAGCGACCGAGTGATTTTCTCAACGCCGTTTCCCTCATTAAAAAGGGTGATGTACTGTTTGTGGATGAAATCCATGCAGTAGCACCCAGTGTGATGGAGTTAATGTTTCCCGTGATGGATGATTTTCGCGTCCAAGTCTTAATTGGCAAGGACTTTAATTCCAAGATGGTGGAAATGAAAGTTAATCCGTTTACCTGAATCGGAGCCACCACTCAGTTCGGTAAGATTATCAACCCGCTGGAAGATCGCTTTGGGATGATCCTTAACATTGACTACTACAGTAACCAAGAGATCGAGCGGATTGTAAGCATCTATGGTGAGCAAATGGAGTTGGAATTAAAACCGGAAGAAATTACCCAGATTACCCAACACAGTAAGCAAACCCCGCGGATTGCCATTCGAATTGTCAAGCGTTTGTTTGAACAGAAAATAGTCAACAAAAAGATTGATCTGGCTGCTCTGTTCAAAAGCTTAATGATTTACAAAAATGGTTTACAGAGCATTGATGTGCAATATCTCAAAGCACTCAACGGGCAGTACGAGCCTCAGGGTATTAAGTCAATTTGTTCGATGCTAGGGATTGATAAATCCACTGTCGAAAACAAGATTGAGCCCTTCTTGCTGCGGGAAAACATGATCCAAAAGACCAAGAAAGGGCGGATCATAACCCGCACTGGGCGTAATTATTTAACTAGCTGCTAACTTTTTGGCACGCTGGATAATCTCATAACCAAAGCTTTGGTTAATCTCATAGATCATTTGATCTAAAGCAAAGTGAGCAGTCTGTTGTTTAGGCTTAGCTTGCTGATACTCATAAAAAAGTAACCCTTCTGGTCTTGAACTGGAGGGGTTATTTTTTAATCCAAAAAAACTCACGCCAATTAAGCGGATGTTAAGCCCCACACCCATTAATAGCCTGTTAAAGAGCTTTTTGACTATTACGAGTAGGGTTTGGTAATCAGCGGTGTATTTCTTGATCGATTGAGAGTGGGAGTTGACAATAAAGTCATTGCTCTTTAACTGCACCACTACTCCCTTGCACATTTGAAAGGACAGTTGTAACCTAGCATACAACTCGTTGAAAATACTAGTGAGTTTTTGCTGGAGTTGATTGGAAGAGTAGTTCAAATCTTCCAGCGTTTCACTTACAGCAATTGAGCGACTTTTGACATCCTGCTCGCTTTGCGTATAAGTTTCACCCAAAGCCACAGCGTGCAAGCTTTCCCACGCAATACCAAACACCCGTTTGAACAAGGTCACATCTTTACACTTAGCGAGGTCTCCAATCTTGTGAAAATTATTTTTAAAGGCGTTGTCAAGTTGTCGTTTGCCTATCCCAGGAAGTTCCACAATTGGTAAGGGTCAGAGTTTGCGTTTAACTTCCTTGCTAAAACAGCTTTTAACCCCAAACGGTTTAGCCTGATTGGAAAAGATTTTCGCTAGGGTAAATTGGTTGGAAATACCAATGGAGATGTGAAAGTTTAACTGTTCATAAACGTGGCGCTGAATCCGTTTAGCCAAACTAAATGCTTTACGGGGTGAAAGGTCACGAAAACACACAAAGCCCTCATCAATCGAAAGGGTGTGTACCTCTAGGTTAAACTGCTCAGCAATCATGGCAAAGATCTTAGCGGAATACTTGCGGTAGTTACGAAAGTGTGATGTGGCAAAGATCGCTTGGGGACACAATTCCAAGGCCTTAGCAATTGGCATGCCCGACTTAATGCCATAGCTCCGGGCAAGGTAATTACAAGTCGACACCACACTCCTAGATGTCCGGTTACCGACAATTAAAGGTTGATTTTTGAGCTCGGGGTTTTCTATCTCTTCTACTGATGCAAAAAAGGCATCAAAATCAAAATAGAAAAAAAGCAGATTAGGATCAGCTTGTATGGTTGGATCTAAATATGTAAAAGTGCCGATCATTTTAATTTAGTTATGTTATTATTAACCTGCAATAAACCAAAGAAAATAACGAGTTTTTAACTTTAAAAAAGTTATCGAGGATTATCATCGTTTGTGCTATTGCAAAAATAATTTCTGATGGAAGCTAAAAAAGATAAAGCTCAGCAGGTTGCTGATGTCTCCCATTTGCTTTCAACCAGTGCAGGATTTGTCATTTTTGACTATACCAGCATGTCAGCAATTGAAGCGACTTCTATTCGCAAGAAGTTGTTTAAGAATGGCAGCAAAATTAAGGTAATTAAAAACAATATTTTGCGCCGTGCTTTAAAAGCTGGTAAGTTTGAAGGAATTGACGAAACTGCCATTAAAGGCAAATTGGCGGTTGCTGTTGGAGTGAACGAAATTGTAGAAACGTTGAAAGCTGTTGACGGTGTGGTAAAGGCGAAGGAAGCGATGAACTTTGTTTGTGGTTACTTTGACAACCGTGCTTTTAACAGTGCTGATTTAGAGAAGATTGCAAAACTGCCAGGACGAAACGAACTTTATGGGATGTTTCTCTCGGTATTACAAGCTCCATTGCGCAAGTTCTTATACGCTTTGGAAGCAGTTAAGGCTGCAAAATAATAAACCCCTCATAAATAATTAATAAGTAAATATAAAAATGGCAAAACTAGATAAAAACCAATTAATTGAATCGTTGAAGGAAATGACCATCATGGAAATCGATGAAATCATTAAGGCTGTAGAAGAAGCTTTTGGAGTATCGGCAACACCTGTAGTAGCTGCTGGTGCTGTTGGTGGTACACAAGAAGCTGCTAGCGAAGTGACTGTGAAAGTTACTGGTTACACTGACAACGCTAAATTAGCTGTGTTAAAGCTTTACCGCGAAATTGCTGGTGTTGGTTTAATGGAAGCTAAAACTGCTGTGGAAAAACTTCCTTGTGTTGTTAAGCAAGACATCAAACCTGAAGAAGCTGAAGAACTTAAAAAGCGTTTCGTTGAAGTTGGAGCAACTGTTGAAATCAAATAATCAACAATGGCAGTACAACAACGACGCTCAAGCAAACACCGTCGTGACAAAAGACGTTCCCACGATGCACTAACAGCTCAAGCGCTGAGTGTTTGCCAAAAATGCGGTAAGAAAAAACTGTTCCACCGAGTCTGTTCGTGTGGAATGTATGGTGATCTGAGAGTAAAAAAGGCTTATTAAAAGCCTTTTTTATTTGCCTTCAACAGCTGTTACTTGTGTTTTATTTAAAACAGCAACGTTGCGTGATTTTAAACGACGCGCACGATTAGTAGAAATAATACCTTTACGTGCTAAGCGATCTGCTTGGGAATAAACATTACCAAGGTTGTCTAGGTTAATTTCCTTGTGAAAATTCTTCACGTTGGTCTTTAACTTAGTTTTCTGTCCCTTGTTATTCAGATTACGTTTAATGTTTTGTCGTAAACGTTTTTCGTTCGATTTGATATTAGCCATTTAGGTTTTTTCGCGATTCACAGTATATTATTATCTTACTAATGTAGCTAACAATCATAAATGAATGGTGGTGGACAACAGGGTGGTTTTTTTGGCCTACTCGTCATAATTATCCCTGTCATATTGTTGATTGTCTTTTTCTCTAAAAAGAAGAATTCACAAAAAACAGAGTTTGGTGGTGAAGGGGGCAGCCGTGCTTCCAAAAAAGATGAAGTTTGAAAGACAGTAAAGCAATTTCTCCAAGAACAAAACGAACGCGGCAAGGAAATTATCAAAACTTTTGTCGCTAAGAATCCTAATCCCTTGCACTCCAGGAAAGACCGGCAGTTTTTTAACCAAGAAGTGCAAGCTTACATTACAGCCCATAACTTAAGCAAAACAGCAGCCAAGCGTTACCGTCACGAGCAATTAAAACTCAAACAACGTGAACTGTACTGCATTTACTTCATTACCAAGGACGCTAAAACCAGCGTTTTTGATGAAGCACGCATTATTGAAGCTGAAGTTTACCAAAAGCCCAATAAAACGGGTAAGGGTGCACCAGAACGTTTAATTCGAATCTTAGGATTGAAAAACTTCAATGACGAAATGAAGTGGATTCAACCACTAATGGATCGCGAGGAAAAACGAAAGGAAAAAGAAGAGAAACGCAAGCGGGAATTGGCAGCACGTCAGTTAAAAAGACAAGAAAAGAAAAAACAAAAAACTTCCAAATAACAAGATGATTAAAGTGGTTTTCTTTGGGACATCCACCCTTTCAAAGTGTTGTTTAGAAGCAATTTTTCAAGATCCTGAGTTTGTGGTTTGCGGCGTAGTGACCCAGCCCGATAAGGTAAACGAGCGCAACAATAAAATTAACTTTTCTGCAGTTAAACAATTTTGTATAGAAAACAACATTCCCTGTTTTCAACCAGAAAAAAACATTCAGATTAAAACAGAGTTAGCCCAACTCCAAGCTGATATTGGCGTTTGTGTAGCCTTTGGCCAATACATCCACAACGATATTATTAACCTCTTTCCTTACAAGATTGCCAACCTACACCCCTCAAAATTACCACTACTGCGTGGTGGAGCACCATTGCACTGAACCATTATTAACGGTTTTACGACCTCTAGTTTAAGTGTGATTGAACTCGTACAAAAAATGGATGCTGGTCCGATTTGAAAGCAAAAGGACTTTAAGGTGAATCCCAATTGGAATACCGGTGATCTATTTGAATACGTGCAAACTCACGCACCGCAATTTCTGATCCAGTGCCTTAAGGAAATTGTAAGTGGTAAGAGTCAATGAAAAGAGCAAATTAATCCACCTACTTTTGGTTTGAATATTAAAAAGGAACAAGAAAGACTTGACCTCAATTTGGAACCAAAAGCATTCATCAATTGGGTGAAAGGGTTGGCACCTAAACCTGGTGGTTGACTGGAATTTGAAGGACAAAACATTAAAATTCTCCAAGCAACTTACTTGGGCAAAATGACAAGTACAACCGCAGTTGGTCAAATCACTAAAATTTCTAAACAAGGTATAGAAATCGCCTTAGCTAATGATGAAATTGTTCTACTCCAAATAATTCAAATTCCGGGAAAGCGGGCAATGGGAGTGTCAGAAATAATTAACGGTAAACATCCTTTTGCTGAAGGTAAATTTTTTCATTAATGCTCACCAAGGCCTTTTTTTTAAAAAACTTTGATCGCTCAAAGGAGTTGATTCCTCTGTCTTACGCTGATGTTTTTGGCGCTGCTAGTCAATTGTTAAAAAAGCACCATAAGCAGGTCGATACCGAAATTGATGTGCAAGAAGATCTAATTGAAGATCCGGTATTTGAAATAGATATTTTAGAGCTACTCAACGAAGCGCCAGAACTATTATTTGATAGTAAAAATCCTCGGGTTAAAGAGGCACAAATCATTATTGAAAAGGCCAAAAAAGATATTGCTAGCTATTTCCACTTAGACAATATTTTAGACACTGACAACTTAGGGTTAAAAGCCACTGTTACCGAAAAAATCCAATTTACCGAAAAACAAATCGAAGCTGCAGTACAAAACAAAAAAGCAGCGATTATTTTTAAACCAGTGTTTACCGTCAACCAGTGTTTAATCCAACCAGATGCTGTAGTAGTACATGCTAATGGTTTGTGTGAGTTTGTTGTTATTAAAGCGACAACAAATACCAAGAGAAAATTTATCTTGGAAATTATTTATGACTTCTTGCTGTTTGAAAAATTGGGCAAATACAAACTGGTTAACTATTACTTCTGCATAGTTAATTATGAACTGAAAAATAAACACAATGTTTCCTTCTTTTTAAACACCGAAATTAAAACAGCCAAAAACAGTAGTACGAGTAAAACTAAAGAAGAGCAAGTGTTGTACGGGCATCTCCCTTTTAATGATCCAAAAAAAATTGCTTACATTCATAGCAAGAAGAGTGGTGGTGTCAATGGTTTTCTGCTCGTCAAACTAGTGGACAACATTATTAGAAGCGGTGTTACTAATTTGGAACAAATTATTAGTTTTGTTTTTCGTGAACTGAATGCACCCAGCATTCGTAGCTTAAAGCAAATTATTAGTGAAGTAGCTAAAGTACAACTTGATTTTTGGAACATTATTGATGATGTTAAACAACACCAAGAGTTACAAGACAACCAAATTACCTTCAATTACAGTGATGCCTTCAACTCTTTTTGGAATAACTATTTATTGCGCAATCTAATTAAATTAGTCTTTGCTTATAAATACAGTGAAATCTTTCGACTTTCAGGAAAATTAGCTAAATGAGATGAAGTAGTTGAGGCTTATAAAGAAAACAAATCAGTGAAAATTGATGGCTTTTTATACGAACTTAACCAAGGGAAAATGAAAAAAACTAAAAATCCCGCTACTTCCCAATTTAACAAAATCCACTTTTTTCTAAGAGCTTGAAACGATAAAAAGGGGATTGCTGTTGGTAATAAATTTAAGAGTGTTTGACAAAAGCTCAAAGAAAAAAAAGTTTACTTTGATTTTGAAACAATTAGTTCTGCAGTTAGAGTAATTGATAAATCCTTACCCTTTACCCAAATTGTGACCCAGTGTTCTCTTATTGTTGATGACAATACCGAGTCTGATAAAAGCAAGTTAGTTTGTCAAAACCTGATCTTTGATCCGCTAACTATTGGTATTGAGGACTTTAAAACAGTCGTTGATGCCCTTTACCAAAAGCAGTGTGACCAGTACAGTTTTGTCGTTTATAACAAATCGTTTGAAAAGAACAGGTTACTGGAAATGGCTACTTTTATCAATGAAGCTCCATACCAACAAAGAGTGCAAGCAATTATTGAAAATTTATTTGATTTAGCAGATATTTTTGGACTTGAAAATGATTGCTTAGCCTTTAAACAACTTGATGGTTTTTCATCAATTAAAAAGGTCTTGCCAATGATTGATCAACGCTTTTTAGATGCTAGTCGAACTGTTAGTTACCAATCATTAAAGGTACAAAAAGGTGATGTAGCACAAGAGCTTACTTTGGCACGCTTTTTAAATTGTTTAGACGAACAGCAATGAGCACAAACTGCGTTAGAATTAAAGCAGTACTGTGAAAACGATGTACGTGCCATGATTGCAATCGAACTGTTTATTAAGGACTTTATTACGAATCAGCTTTAACCTTATTCTTTAAAGCTTTTAGTTTTGGTTTACGTGCCTTTCAGTTAATCTTTTTCGGTCTAACTGTTAAAGCTCGCATAGGCTTTTGACTGTCTAACTTATTTTTAATGTCACTAAAAAAGTTAATGCCTTTACCACTTTTAAGAAACTGGGTAAAGGCTGACATTGCATTGTGTTCTAACACGCTCTTGTCATCATAGTGTTTACTGTTAGGTACAGCTTGTGACCAGATAATGTCACCGTTAAGCTCAATAATACTCAACACAAGGTTATTGCTAGTTAAACTAACCCGTACCCTAGGTTCTGGTAGCTTTTGTTCTTGAAACAGTGAAAAGAAGTTGTACTTGAGAATTTCTGAGTAAGTTTTTTTAAAAATATGTTCTTCGACAAATTGATTCGCTTTCTCTATGCCAAGGTCTTCGTAAATGGCTGCCACTAACGCCTCTAGTACATCACCAAAAAGTGTTTCAGTATACGGCATTCCTTTACCTAACTTAATGAAATTGCCAAAGTTCAATTCCTTCCCAATACGGTTGAGGTTTTCGCCCTTAACAATTTCAATCTTAGTTCTGGTCAACATACCTTCGTTGTAATTGGGATAAAGCTCAAACAGTTTTTTCGCTACTATGAAATCAATTAAGGCATCACCTAAAAATTCCAATCGATCATAGCTTGCGCGTGCATCTTCATTTTCATTAACGTAAGAAGAATGAACAAAGGCATCTTCGTAAAACTGTCAGTTTTGCGGTTCAATGTTTAAACTCTTGAAAAAAGCGACAAACTTTTCATCAATGACCTTAGGTTTTTGAGTTTTATCTTTCTTATTTTTCATAGTTATCTAAGGAACTTTTAATGGCGTTAATTAGATCAGACTTTAAGCTAGTGTGTGCTAATCTAATGGTGCTTAAAAACTGTTGTTTATCAGCTGATCCATGGGTTTTTAAAGCAAGTTTATTTAAACCGATAACAACGGCGCCTGCGTTATTTTTGTAATCAAAACGCTTAGCAATTCTTTTCAAAATTCCCAAACTAAAAAGCCCAGCCAACGGGTTACGCTTATAACCTTGCTTGAGAAGACGCGCAATTGTTTTAAAGGTGCCTTCCATTGATTTCAACACAAGATTACCGCTGTAACCATCAGCCACTAAGATATCACACACCCCGTCAAGTAAGAAGCGTGGTTCAATGAAACCAGTGAAGTTAAGGTGCTTATCTTCGTTTAGCAATTGGTAGCCTTCCTGGTGGTAATCAAAACCTTTATGGATTTCGGTACCAATGTTTAGCAATGCTATACGTGGACTAATTTTGTTCGTGGTCTTTTTGACAAAAATATCCGCCATTAGGCCCAAAAAATAGAGCTCTTTACCCGTAAAGTTCTTGTTAGCGCCAACATCTAAAAAGTAAAACCAGTCGTTATTGGCAGTTGGTACATAAGACATAAAAGCACTTTTAACATCTTTATGGATTTTGCCAAAGCCATCGTATGTTAAAGAGGCATAAACGGCCGAGGAGCCAGCGGAAATCACAACATCAGCAGCACCATCACGCACAAGGTTAATCGCTTTCTGCATTGAGTTGTTAACCTTGCGCCGTGCGGTTAGTGGTGTGTCGGTCATATCTATGCAAGAACTGGCTAATTGCTTGGTAATGTTTTTAGGTAGATAATCAAGTCCGTCAAAGGTCTTTTCATCACCAACTAAGATAAATTCCAACTCTTGATGATAGCTCCAGTATTCTAATACTGCGTCAATTGCTTCACGCGGGTGGTTTTCAAAACCTAAACAATCAACTGCTAGTCTAAAAGCCATTATTGTATGCCTAGTATATAAGAAAAAACTTTTTGTTCCCCTGGGGAAAACTCACAGAAAAGCTTGTATTTCTTTTCTATTTTTTCCTTCATTGCTTCCGCTTCCTCAGCGGTGATATCTTTACCATAGTAAGCTAAAAGAAACTCCGGCTTTTTTACCTTTTTCAAAAGGTGGTCAATGGTGTTGAACACGCATTGAACAAGCTCTTTTTCACTAACACAAATGCTGTTATTAGCTACAGCAATAAAGTCACCCTTATTAACTTCAATCCGATTTTCCTTGTACTTTTTCGAAGCTTGAGTAATGGTTGCAGATGCAAAGCCTTTGACAAAACGGCGCATTGTTTTGACATTGGTGTGAATGTTAAGGTCACTGTTAAAAACAGTAAGCGCCGCTAGTGATTCAATCGGGTTGGTAGTCATCACACAATCAGCACTAATCTTTTGGTGCTTTAATTGTTTTAACGCTTCGTTGGCAGAGAGAAAGTAGTTCTTGTCATGTAACAAGAAAATAATGTTACTGGAATGAGTTAGCTTGACTGCTTCTAGTAACGAAAAGACCGAAGGTGCACCGGTGTCATCGGTACACAAGATAGCTTGAATGTCATAGTCTTCGCGAATCCGATCAGCAAAAGCTTCAGTTGGTACAGTCACTACTATGGCTGGTTTAATGTTGCGCTTCACAGGAGCGGGTTTTTGCTTCGCTACCTGTAAATTCATGTTTTCAATTTTGACGAGTTCAAACTCACCATAGTTAAGCCCAAGTTCTAATAACAGGTTAGGTTTTTCGGTATGGGCGTGTACTTTAACAAAGCCGTTATCTTTGTCACTAGCAACTACTACCGAAGTGGCAATTTTGCTCACCTTACTTTCAAACTTCTTTTGGTTAAACTTCTGCTTTTCAACTTCTTGACTTACTGACATGCCCAGTTTCAAGACATATTCAGTGCAGTAACCAAACTCTTCTTCCGTAACGTGTTTTTCACCGCTAATGGTCATTTGGCTCAATTCAGCACTGGTGAGCTTACCATCGTTTAAAGTAGCCTTTTCACCATAAAAGGACAACATCCCCTCTAAAAAGCAAGCGAACCCATAAGCACCTGAATCTACTACACCAGAGGCCTTTAACACTGGGAGCATTTGCGGAGTTTTTTTAACTGTTTGTCAGGCTGTTTTAATTACTTGTTCAAACAGTTTTTGCACTGTTTTGGCACGGTTCTTTTGGTTTTTAAAGTCTGTGCTAATTAAGCGAATAACCGTGAGCATCGTACCCTCTACGGGCTTGGAGACATTCTTGTATGCCACCTCTTCGGCCTTTATAAAAGCTGTAGCAAAATCCTCTATGCCAAGTTCGGTTTCGGTGGCTGTTTTAGTGCTAATCATGTCACTAAAAAAGCCCTTCATAATTTGGGAAAAAATAACACCAGAGTTACCGCGTGAGAAAAGTAAAAGGTCACGGGTAAAGCTTTTGCCCAAATCACTAAAGCTCTTAATGTGGCTAATTTCACTCTCTAGTTTTTTGAACGCTTCACTCAACGTGACCTTCATGTTTGTCCCCGTATCACCATCTGGCACCGGGAAAACATTGAGTTGATTAATGTATTCGAAATTATTAGCTATATTCTTACAACCAAGACGCAACATGTCAATAAAGTTGCTTAAATTTACTGTAGACATACGAAGTTAAAACCTGTTTTATCTGTGATAAATCGGTTTATATTGCCATTAATTTTAAATTGAAACCCCTTTAATTAAACTAGCAAATCACGGTACTAGTTGGCTTTTAAACTGCCGTTGCGAGTAATTAGCCAGTAAGGGAAATTTCTGCTTATCTAATTGGGCAAAACTTAGATTTGTGGCATGTAAGGCTTGGTGTTTAAAATCGCGGTTACGGTTTTTAAATTGCAGTAATTGGTATTTTCTATCACCAACTAACTGGGTATTCAAGAGGTTTAAGCAAGCACGAATTTGGTGGGTTTTACCACTTACTAAGCGTATAGTAAGCAACGAAAGATCTAACTTAGTTAAGTACTTACTGTTTTCAAAAATGGTGGTAATCGGTTTAGCGTTAGGAAATGGTTTGGCCTTAACTGTTACCAAAGCTTGATAAGCATCTTTCGCTCAATAAGCTTTTTGTGTGCCCTTAAAATTAAATGGGCCAAACACTAATCCTTGGTATTGTTTCATTAACAAGTTATGGCGAAAAACCGCCTCTAACTCCTGTAATGCCTGGTTGGTTTTAGCACCTATTACCAAACCACAGGTATTACGATCAATTCGGTGCGCAAACCGTGGTATGAAGCTAAGACTGTTTTTAAAGTATTGATTATAACCACAGTGTTTCAGTAAGCTGTTAGCTAAATTAAACAGTTCGTGTTTAGCATCGGGTTGACACACTACACCCGCTGGTTTGTCCACCACAATGAGCTGTTCATCCTCAAAGATTACCTGCAAATCATCTTGGACTTGGGTTAAATCCACTACTTCGCTGTGGTTGTGTAAGTAAGGTTCAATTGCAAACTGAAAAACGATTGTATCACCGCTGCTAACACGCTGACCTAATTTAGCCTTCGTGTTATTGAGCAAGACCTTACCTAAGCGCAACAATTTAAAGAACTGACTTAGCTTAATGTTGGGCAACAACTTTAAACAAAACTTTTCAATCCTAACGTTTTCAACAGCTTTAGGCACTACAAACTTAGCTGTTGACATGCTTTTGTTTGGCTAAAAAGTCATCCATAATTTCCACTAACTTCATAAACTGACGCTTGTCATCCAACACAAAGGCAGCAGCATACTTGTGCCCACCACCACCAAACATTTGCGCAAAGTTATTAATCGGAATTGAGCGTGAACGAATGGAACCAATTCACTTCTTAATGTCTTCGTTAAAGTAACAAGTCGTCCAAATTTTGACTCCTTTGATGTTGTTTAAAGCGTGCACCATCGGTAGTGGTGAGACCACCCCAAACTGCTTATAAGTCCCTTTTTTAAGAAGTGCGTAAGCCAAACCATTCGGGGTGATCTTGGCTTTGTTTAAGACATAGCTAAAGTACTTGTGTTCTAAAAGTGGTTTTAGATAAACTGCATCATGAACCTTATTGCGGTTAAATCCAGTTTCTAGTAACTTAGCAGTTAAAGCAAAGGTTTGTGGGGTGGTAGCGGAACTGGAAAAGCGCTGTGTATCGGTAATAATACCCGCATAAATGTAAGCAGCCATCTCAGCATTAAGTTCATAACCCATTTGCAAGATTAAGTAACCGACCATTTCTGCTGCTGCTGAAAAAGCCGGGTCAATCCATTCCAGATCAGCAAAACTCTCGGTTTTAGGATGGTGATCAATCCGCACGGTTTCTTTAGCTAATTTATGTTTTTGGGTTAATACTCGTTCTTGGTTGGAGGTGTCAAAGATAATTGCCAATGAGTTTTTTACAAACGCATCATCAATAGGTGCCGCTTCAAAGGGGAACAAATCTTTCCCGTCAGCCTTAATGTTGTGTGATCCCATCACGTAAGCCTTTTTATGGGGGAAGTAAACCGCTAAAAAGGCCTTAAAGGCAAAAGCAGCGCCAAAGGCATCAAAGTCCGGGTTTACATGCACATATAACGAAAAGTTATCGAATGTCTTGAGTTTATTAGTTAGGTTATGAATAAAATGGGGATCGATGTTAATCATTTTGCAGCTTCTTGACACCAGCTTCAATTAGTTCACGGACAAACAAAAGTTCACTCTCAAGCTTTTCAACGATCGCTAACTTAGCCTTAGTAGTTGGTTTTTTGGGAGCAAACACAGTACAGGTATCCATATGGTGCTCAATTGAAGTTTCAAAGGTACCCAGTGCCTTAGCTCATTCAATTATTTTATCTTTACTTAAGCCTATTAACGGGCGAATCACAAAGGTATTGGGCACAACAGCTTGGATTACCTTCAAATTATCAATTGTTTGTGAAGCTACTTGTCCCAATGCTTCCCCGGTGATTAAACAATCATATTTAAACAAACTAGCAATCTTGTAAAAGCAACGGCGCATTAAAACAATCCGGTATCCTTCCAGACTAATATGACTCAACTCTTTCTGTAAATCGGTAAAGTCAAAGACAAACAGTCTCCCAGTACAAAGCGTTTGGTTAAGGGAAACTAAGTTAGCTAACCGTTGAATTTTGCCTATTGTGGCAGCGTTATGTCCGGGCTCATTAATAAAAGTAATAAAGTCAACATTAAAACCTCGTTGTAAAACTAGGGAAGCGGCTACTGGACTGTCTATGCCACCAGACAGTAACGCCAAAGCAGTACCACTAGTAAAGACCGGTAGACCACCAATACCATTAAAGCTTTCACTAATTATTAGGAAATGTTCCTTAGTTACTTCGACATGCACCGTAATGGCAGGGTCAACTAACTTACCCTTCAATTGGTATTTTTCCCATAACTGTAAAGCTAGGTACTTTTTGAAGGCACTGGAATTGCAAGCAAATAATTTATCACGCCGTCTTACCTCAATTTTGAACGATGGGTGGGATTGTACCACTTGCTGTAGCAGTGCTAACAATTGCGCTTCATCATGAGGGATTTGTAAGGCAAACAAGCAAACACTCAAACCCGGTAATCTGGTAAAGAGATTTTTTAATAACGATCTTTGGGTGGAAATTACTTCACCCACTACCAACATGTCAAACTGGTAGTCAACTGGGTTAGTAATGCTTAGTTTCTTAAAGGCTTGTTTAACGTTCTTTTTGAGTTGTTTGACAAACTGCAAGCGGTTCTTGCCCTTTAAAACAAGTTCGCCATAACGGCAGATTAGAATAGTATTTGGTTCATTATGCAATCCCATTTTCTCTCAGTCACTCCCAGTCTTGTGAGGTTAATTGTGGACTTTTACCAGTACGAATAAAACTGTTGTACCACAGCTGTGCTTTATTAGTTAAAACTTCAAAGGCCTTGCGATCAATTTGTACTGGTACCAAACGGATGTCGCGTTGCTTTGCTTCAAACCATTCGGGATGCACATAGTCTTGTGGTTCTAAAAAAGCAATCGCAAACATTCCTTTGGTAATATTGCGCAAGTACAAATACAACCCAATTTGGCAATAGTAAGCCGGGCTAATCACGATTTTGCCGTTACTGTCAAACCAACTATCTTTCTTTCCGTTAATCTTTTTGACAATCGGCATGCCGTTTTCATCAAACACAATTTTGAGGTTACCGTTAATCTTTTTATAGACCAAAGAATCACAGGAAGTGGTTTTAATTTCTAACATCGGCGCATTGGTATCGTAAGCCAATTTTCCATAAACATCGACCGGTTCCCCATCAGGAATTCCTCCAAAAACTGGATTTTCAGGAAAGAGATCCCACTTAACCTCCTTGGGATCATACGAATGGAAATTAAAACCAGTTTTAGCTATCACATAATCACGGATTTTGGGTTCAATAACTTGCCCCGCGCGTGAGAGTGTTTCATCAAATTCATCCTCATAAATCTTGACCATGTTAGCTCAAGTTTTAAGTGAAGTTTCATACTCAGAAATACCTATTACTTTACCAAATCGGGTACCGGTAATCTTGCGAAAACTACCTTTATGTTTTTGAAAATAGTTGTTTGACAAAACTATTTGGTTGTCAATAATATCAAAATCGATTTTGTACTGTTTGGTAAATCCCATAAATCATTACAAGAGTTGTGCACAAAGCTTTTGTAACACATCAGGAATGTCCTGCACTGTTAAACCTTCATTGTTCTGTTTCTTGATGAAAACAGCACTAGCTAACTGCTCACTGTTTTGCACCAAAGTTTCATCTAAGTTAATGTAGTGCACATCTTTAAGCATTAAGCCTTGGGTCTTAGTGTATTTTTCCAGTGCATTAAATTTAGTTACTTTATTACTTGTAAACAACAGGTTATTGGGATAGAAATTGCTGTGGGTAAAGCGTGGTTGACCACTAGCAATTTGGTCAATAAAAGCTAAGTCCATGTGGTATTGGGGACTAAAGACGTAGATAGAACTGAGTTCCAATTTACCGAGTTGTTTTAAAAAGTTTTCGTACTTTAAGGTTTGTTCAAAGTCCGTTAACCACAGCGACTTAAAGTCCTTGTACTTTTGTAGTGAAATGAAATAGTTAGCTGATAAATTAGCGGTATCATCAACGCGGCTTTTACCCATGGTAAAGATCCCGGAATTGGTAATCACAGCGTGGTGGATAATGACATTAATACTAGCGAGGTCTAAAAAGGCTTGATAGTCATACTGATTGGTTTTGCAAAACCAGGTAATGGCACCTGATTCCGCAATTACATCAACGTTGTTGAGTGAAAGTAAATCTAACAGATACACAATTGTTTGCTGTCAGCAACTCGAAATAAAAACGAGCTGGTGCTTTTCTTGTGCCTTATTGAAAAAATCCTTTCAAATTGCTAGGTCGTTAGCAGCGTCAAAGAAGTTAGCTGGATTAATATCTAGAGCAACAATCTTTCTATCCATCCTTACGCACTTTTTTGACAAAGTTAGCTCAATACAGTTTCTGTACCTTCTCACCTTTAAAGCCACGACAAGTTATTCGAGTAAGATCCTGCATCTCCTTATCGCCGACAATAATTTGCAGTGGAATCTTGTTAACAATGGCTGTGCGAACTTTTTTGCCCAAACGATCTTCGCTCTCATCTAGGCTGACCCTAATGTTTTCCTTTAACAATTTTTGGTACAGTTTTTTGGCAGCCTTTTCGTGTTTATCGATGTTAACCGGAATCACCACTACTTGTACTGGTGCCATTCACAACGGTAGATTGCCATGAGTTTTTTCCAATAAAGCAGCAATAAAACGCTCGTACGTACCGACTATGCCAACGTGAATAATTACGGGAGTTTGAGCATTGCTCTGCTCATCAAAGTAACGCAATTCAAAGCGTTCTGGCAGTAAAAAATCCAGTTGAATAGTGGCAATGGTAATCATCTTATTGAGTGCCGTTTTAAACTGAAAGTCAATTTTAGGTCCATAAAAAGCAGCTGCACCCACATCAATTTTATAGGGCACATTTAACTCCTTAAACACGCTTTCAATTTGGCTTTCTGAGTTAGCTCATAACTGATCGTTGTCAATGAATTTGGCCTTGTTATTAGGATCGTGTAAGGCCAAGTCAATTCGGTCAAAGCTAAAACCAAACTTGTTGTTGACCGTTTGGATTAACTTAAAGGCATTTTTAATCTCAGCTTTAATTTGGTCAGTACGACAAAAGATGTGGTTGTCGAGCAGTGTCATGCACCTTACCCGTTCCAAACCAGTAAGCCCACCCGATGCTTCAAAGCGGTGCAAGATGGAATCCTCACAGAAGCGTTGTGGTAACTCTTTGTAAGAGCGGCGTTTGTGCTTGAAAATCAAACAGTGGTGCGGACAGGTCATTGGCCGGAGCATCATCATTTGGTTTTCTAATTTGACAGAGGGGAACATGTCCTCACTATAGTGCACATAGTGACCACTAATCTTGTACAGCTCTAAGTTAGCTAATACTGGTGAAGATACCGTTTTAAAACCAAACATTAACTGTTGGTAGTGGACAAAATCACCAATAATCTTTTTTAAAACTGCTCCCTTTTCCAATCAAATCGGAAAGCCTGCACCGACTAAGGGATCAAAGGTAAAGATCTCTAAACGCTTACCAATAGCACGGTGATCATTTTGGTATTTTTGTTCATTAGCCGCAATAAGACTTTTAAGCTGTTCTTTAGAGGCTGCAAAAATTCCCACAAGCCTTTGTAGTTGTGGTTTTGTGGGATCACCTAAAAAATAATTGGCACTAACATTGAGCAGTTTCACAAAGCCCTGTTTGGGTAATGGCAACACTAAATCATGCACCCAAAATGTTTGTTCATTTACGGTGGTAACTACAAGGCTGTTGCTTTCTATACTTTCAATAAGCGTGGTTGTAAACGGATCAGCTTGAAAAAATTTGAGTGATTTTGTCTTATCAATTTTTGTCTGAACAACATTATTTAACTGATTAAAATAAGCATTTAAGTCCTGTTCGAGCTGTTCAAACTCTTTAATGGAAAAGCTCTTTTCCACCTTAAAGTCAAGGTAAAACTCGCCTTCTTGTACCCCGTAACTACCAAACTGTACTTGAGGATAGTTAGCTTTTAATCAGCTTTGTAATAAAAGAACACCAGCTAGCATTAGATCTCTTTGTAATCGATAACTATTTCGTTTTTCGGATTGAAAATCTTGACAATTTCAATTGTGGTCTTTCAAATTTCACTCTTACGCTCTAAATAAGTGCGCAAAATGCCTTCAATACTAATGGTCTTGTACTTCTTGGTGTGTTTCTCCAATTCGTAAGCCAGTTGACCATTGGCATAAATGACGTAATAGTCAGTAAATAAACGCTCGCCAAAAAAGCGCATTTGCTTAATGGTTACTAAAAATCCAGTTTTTTTAACGCTTCAGCACGAACTTTCTATCTCACCCTCCAAAAAAACGCGGTTAAGCATATTAGTTTGGTAATTGTCCAGTTAAGTTAAAGTGAAATTTAAAGTGGTTGATGGTTTCTAACACCATGCGCTCTTCTAGCAAGGCTGTACGCACACCCTCAAACTTTTGTTTATCATCCAAGTATTCGCGCACACTTTGATTGGTTTTTTCGTAATAAAGACTAATTACATTCTTCACCACTTCATCAGTGATTTCCACTTTCCATTCTTTTTGGAGGTGGTTAAACACTAAAGCCTTCTTAATAATTTTTTCAGCAATTGCTTGCACGGTGTTGTCCTCAGCGCCCTGAATCACATCACGCTTTAGACCTTCCATGACATTCTTCAATTCAGCTTCATCGATGTTGAATTCAAAGTAACCCACAATAGCATTCATGGCACAGGAAAAGAGGTTGTCCTTAATGACAATGTGCGTTAGTTCATGATAAAGCGCACTTTCCTTAGCGTCCTTAAAGGAAGCTAAGATCCGTTGGCGGTGTTGTTCAATAAAAGCAGGGTCAGCAAAGATGCGTTCTACTTCAATTACCTCATCGTACTGAATTGGTTTAACTAGTTTAGCGGTTGATTTAAGATTTGTAGCCATATTTAATATCGTTTAAATTGTAATTCTTAAACCAACGCCCTTTTACATTTGTTGTGGTGCAGTAATGTTTAAGATTTGCAATAGCTGACCAAAAACATGGTGCAATGATTGCAGTAAAAGTAAGCGTTGCTGGGTTAAATTGTACTGCGCTGGATCGTTAATTTTGCAAGCCTTATACCAAGAATGAAACAAGGTAGCTGTCTCGTATAAGTAATTAGTTAAGAGGTGCAGTTCATGGCTAACCTGAACTTTTTCCAGTAAGCTCTTAAAGTACACTAACTGGTCTAGTAAAGCAATTTCCTTTTCATGCGTTAATAAGCTGCAGTCATTTACTTGAGCTAGTGCTGCTTGATCCACTACCTTTAATACACTGCACATTCTGGCATACGCATATTGCACGTAATAAACCGGATTGGAAGAGTTTTGCAGGTTAGCAGTGTCCAAATCAATCTTGATAATCGAGTTGTTGGTTTGCGACAACATAAACCAACGCGCCGCATCCTCACTGAGCATTTGCAACATTTGCTTAATGGTAATAACGTTGCCAGCACGTTTGGACAATCTGACTATTTCCTTATTCTTGTACAGCATTACCAACTGTAAGGCAGTAATTTTGAGCTGGCATTTTTGGTGAATCTTTAGTCCCTCATAAACACAGTGCATCCGTTTAATGTGCCCTTCATGATCACTGCCCCAAATGTCGTATAAGAGGTCAAAGCCCCGCGCAGCCTTGTCTAAGTGGTAAGCGACATCGGTACCAAAGTAAGAAGGCCGATTGTCTTGACGCACTAACACCCGGTCCTTATCATCACCATAGAGCGTAGTCTTCAATCAAATGGCACCATCCTTTTCATAGTGCGCTTCGTCCTTAAACTGGGTTAACAGTTTTTTGTAATCTGTTTCCTTAACCACTTGACTTTCAAACTTCCAGCTGTCAAAGTGAATGTGGATTGTAGCTAAGTCTTGCTTAATTACTTCCAAGACTTTGGCAGTACAGTCAGCTAGTAACTGGTCCAGAAAGGGTTGTTGTAAAAACTCTTCAAAGCTGAGTTTATCTAAAGGAATACCGGTAATAGTTTTGGCAATACTGTCCACTAGCTCCCCCTCATAACCATCAGGGTGAGGAGTGACCTTAATGTTTTGTTGTAAGTGGAGTGCTTGGTACACACTAAAACCAAACACCCGTGCTTGTTGACCATAGTCATTGACCCAGTATTCCCGAACTGTTTGAAAGCCTAAAAAAACCATCAGGTTATTGAGTACATGACCAAAGAAGGCATTGCGGACATGACCTAAGTGCAACTCACCCGTGGGGTTAGCCGACACCCATTCAATTAGAACCTTAGTTGGTTTAGGGGCAAAAGTAGGTTTGGTTTTTAAAAAATCGAGGTATTTTTGAGGGCTAATTTCAAAGTTAATGAAGTTGTTATTAGCGACAAACACACGGCGGTAATGCGCCTTTTTGTGTTTTAACAACCAAGCAGCAATCTGTTGGGCGGTTTCCTTTTGATTTACACCCTTTAAGTGGAACACAACGGTGGAAGCAAAACCACTAAAGCTGTTGTTTTTAACAAGTTTGACTAACTCTTTGTGCTCATCAAATTCTAGATTCAAAGCGTTAAGACATTCTTGAAGATCTGTGTTAATGAAAAGCATTACTTAAATTATATTTAACCTTGCAAAATGTCTTTTACACTAACAGTTATTGGTGGCGGTCATGCCGGATTAGAGGCCGCATTTATTGCTAGTAAACTAGGGCTAAAAGTTAACCTCTTAGTACTAGATCCCAACCATGTGGGGTCGTGTCCCTGTAACCCGGCCATAGGAGGTCCTGCTAAAGGTATAGTAACCCGGGAAATCGATGTTTTAGGAGGGATGCAGGGTAAGGCCGCTGATGCCACTGCTTTACAATACAAGCTGCTCAACAGCTCGAAAGGTCCAGCAGTGCAAGCAATCCGCGCTCAAATAGACAAAATTGCTTATCAGAAGTGGTTTCGCCAGCAAATTGACCAAACGCCTAACATTGAGTTAATTGCTGGGGAAGCCGTTGATATCCTTGAAAGTAACGGTAAAGTTAAAGGTGTCGTGTTAGCTGATGGTAGTGAACTGGCATCAGATGCTGTTATTGTGACTACGGGAACTTATCTAAAGGCCAAAACTTACTGTGGTAGCTTAAGCAAAGAAGAAGGTCCTGACCGCGCTAAGCGCAGTGAATACCTTTCCACTAACTTAATTAAGCGGGGTTTTAAAACGTTACGACTAAAAACTGGGACACCACCACGGATCCTACGCGAATCACTCGACTTTAGCCAAATGGCTGTGGAAGCTAACACTACCCCTCATTTAGCCTTTAGCTTTACTACTAAGAATTATTTACCACTAGAACAGCAAGTGATCTGTCACTTAATCCATACCAATCCCCAGATCCATCAGTTAATTCTGGCCAACTTAAAACAGTCCGCTGTCTTTAACGGTAGTATTAAAGCAAACGGACCACTTTACTGTCCGAGTATTGAAGACAAGGTATTTCGCTTTCAAGACAAAGAACGGCACCAAATCTTTGTCGAACCTGAATCACTTAGTTTAGAAACGGTTTACCTAGCAGGGTTTTCCACTTCCTTTCCCCCAGAGGTGCAAGAGCACATTGTCCGTTTACTCCCTGGGTTTAAAAATGCCCGGTTTCAAAAGTATGGCTATGCCATTGAATATGATGCCTTTAGTTCGATACAACTCAAATCAACACTAGAAACTAAGTTAATTCAAAATCTGTATTTTGCCGGACAGATTAATGGTACCAGTGGGTATGAGGAAGCAGCTGGCCAGGGCTTAATCGCTGGGATTAACGCCGCTTTAAAGCTACAGCGTAAACCCGAATTTGTGTTACAGCGCAATGAGGCATACCTTGGTGTCATGATTAACGACCTGGTTACTAAGGAAATTAGTGATCCCTACCGCTTGTTAACTTCGCGCGCTGAACACCGTTTGTGGTTGCGCAATGATAACTTACAAGAACGTCTAATTGAAAAGAGCCGAGCCTTAGGTTTAGTGGAAGCAGATGTGTATGCCAATTACTTGGAACAACAACAAAAGAAAAAACAATTAATTGATTATTTGCAAACCACTACCGTTGGGCAAATTGCTGCCTTGAAATTAAACTTTAAAAACACGGCCCAAACGCTGTTTGACTTTACCAAGCGTGCTGAAATTAAGCTGGTTGATTTAGTACAACTACTCCCCAAGCGTTTTGACTTGGATGTACAAAGTTTAAACCAGATTGACATTGACATTAAGTACGCTGGTTACATTAAGAAGAGTGAAAAGTACTTCAAGAGTTTAAACAACCTCAGTAGTGTCAAAATTCCCTTAAAGTTAAATTACCACAAAGTACCTAACTTAGCGAGTGAAGCAATTGTTAAACTCTCCAAAATTAGACCAACTGATCTCAGTGTAGCTAGTCAAGTAGCGGGCATTAACTTTAACGATATTTTAGCGATTAAACATTTTTTAGATAACCATGAATAACAGTAAATTGAAACAGTATGTTCAACTAGTACAAACAGCCAACCAAAACTTTAACCTCACCGGTCTTAAAACTGAAGGCGAAATTTACGAACACTTAGTCCAAGAAATTATCGAGCTCTTTAATGAGTATGACAGTTACTTTGACCATAAAAAGGTTGCAGATTTGGGTAGTGGAAATGGTTGTCCTGGTGTCATCTTAAAGCTGTTGTTTCCCCAAATCAAAACTTTGGATTTAATTGATAGCAAACACAAAAAGGTTAACTTTTTAAAAGAGGTGATTCAAACACTGGAACTAAACAATACCCAAGCACTTTGTGCCAGAATTGAAAACCACACTGAACAATACGACACTTTATGCTCCCGCGGACTTGGCAGCATTATTGAGGTTAATGCCTTTGCTTTAAAACTGTTAAAGCCCAACGGCATTATCTTCCACATTAAACAAAGCTTAGATCAATACCTCGAGTTTGAGGATTCGGAGCAAAAAGACCAGTTTAAACCACTGTTCTTTAAGTTTTTCCATGGCAAGCGCCAGCAAATCCTGATTGCCATGAAGAAAAATGTTTAAGTTAGGTTGAGCTAGCTTTGTCGATCCAGAGTTAGCTAAAACAGCTTTCTTGGAATTTGCCAAACAGTTTTATGTAACTAGTAGTGCTGGTGCAATTCTGTTTGACCTTAAAAAGAGTCAGGGCTTAGACCAACTGCAAGCAATTGAAAAAACAAGGAAGGTTATTATTACAGAACAGTTTGCCAACCAAACTGGTAAATGAATCTTGTTTGACAAGGAAAACACTAAACGGATTAACAGCTTAGCGCAAGAGCACATTACTCCCTTAATAAAACGGATTTTACGCTTAGCGGACTTTAAAAATGTCTTGATTAATGTGCAGCACCACAAGAAGCTGCAAAAATGTCTGTTATGGGAAATTAACGGTTTAATCTGCTTAGTTGAAAGTTTACAGTTCATGGAAAATCCCACTGCAATCATGGAATGATTCCAGGGATTAAAAAAACACTGTCCTAATGTAGCAGTTGTAACCATTAGTGGTCAACACAAACCAGTCATAGAACCGAGCTTAACCGAATATAAGGCAGTATTTGGTAGTAGTTTACTCTCCTTTCACTTAGATGCAACTACGATAAATAACTCCCATTTAGTGCGTCAAATTTTGGAACAGATCAAAATAAAAGCTACCTTAAAGTCAAACTCTAAGGTAGCTAAAAGTTAATGGCGCATCGGGTGGGACTCGAACCCACGACAACACACTTAGAAGGCGTGTACTCTATCCGCTGAGCTACCGACGCATTATTCTCTCCAGAGAGAGCAAACCATTTCCAAGGGGCCACTAGGACCCCGTGAAAGTTCGGAAGCGGGGATCTTATGGATGATTAATCCCAGTTCATTGAGAAGATTATTGGTGTAGTGGTTGCGATCAAAGACCACAATTTCTCCAGGGCTTAAGTTTAATACGCCCATACCACAGTCCAAGAGTTCTTTACGCTCAGCCACTTCATCACCACCACCAATAATAACAAAGCGGGGTTGTTTATCAATAATCGCTTCAATTATCTCACCAAAATTAAACTTTAGCGGTTTGAATTTAATTTGTGCTAAGCCATCCACATCCCTCTGGGCGGTGGCTGTGAGTTCAAAGATTTCGGTAGCTTGTAACACTTTGCGCGCAATAATGAACTTGTCGACATCCACCATCGCTATAAGTTTATTGAGATTGGTCTTAGCACCAAACTTGTGGATATTAATTGCAATAATCTGCTTAATTGGATTAGTGTGATCGCTTAATAAATTACGTGCCAAGCTTTCAATTCCCAACACATCGGTGTCATGACTAATACCAATTAGCAAGATTTCATTGCTAATTTGAATAATGTCACTACTGTTAATCAAACAGTTCTTAAAGTCACGGTTAGTATAAAGCTTAACTCCATCAAAACGGGGGTGAAAGCGCATGATGTATTCATACACTAACGAATGGCGCTTTAACTCAGAATCATTAGTGACAAATAAAGCGACACCCGTTCCTAGGGTATTGAAGAACTTATGTGGATAAAGGGAAAACGATAGCGGTTTAATTAAAAACGGTGAATTACTTACATGAATGTCGGCAAACCGTTGCGGGGTAATCGGTGGTACATCTGATACCTTAATGCCACCAAACATGACCTTAATCACATCTACAAGCGAATCTAAACTGCGAAAGAAGTTTAAGCAAGCTTCGAAGGTACTCACACTTTTAATGCCTGATTCCAATAAAAAGTTCTTAATGAAGTCCTGGCGCACATTAGGGTCAGCCGCACTTAAAGCTTCTAACAGTAAATCTTGTAAATAGTGCACCTTTGTACCATGTTGGCGCAAAATCTTAATAAATAACTGGTGTTCCTTTTGAGCGCGTTCTAAGTACATTGCACTGCCAAAGGTCTGGTTATCAATCACTCGTTCGGTTTCGATACCGGGATGATGGGCAATTACTTCCCTTAAAAAACTAATTTCACTAGTAACGCGTACACCACTGTTAAACTGCTTCTTTTTTAACTGTAGTGCTTTAGTGTTTGGTTGATCTAATTGGTTATGCCCATCGGTTTTGACAAGCTGTTTTTTTGACATGTAAATTCGATTAATTTTAAATAATTAACAATTGCTTTAATTGCGTTACTTCCATGGATAGTAAAAAAGGAATTTTAGTGGCAATTGGTGGCGGGAGTTGTTCTGGTAAAACTACGATCGCTGACATGATTTACCAACTTTTGCGTAAAAAGCTTAAAGTTGCTATTCTGCCCCAGGACAACTATTACAAACCATACAAGAACAAATCAATGGCGCAACGCAAAGCGATTAACTTCGATCACCCCGATGCCTTTGATTGAAAGCTGTTGTGAAGTCACCTCGATAACTTACTAATGGGTAAAACAGTGGCTGTGCCAATGTATGACTATGTTAATTACACCCGTAAAAAGGAAACCATAGAAATAGGTCCTTTAAATGTAGTCATCTTGGAAGGTTTAATGCCGTGGTTTGATGAACGGATTGCTAAATTGTGTAAGCTCAAGATCTTTGTGGAAGCGACTGGAGAGGAACGCTTAATTCGCCGGATTGAACGGGACTGGGAACGCGGTCGTGATGTCGCTTCTATCATTAAACAATGACGCGAAGCAGTTAGTCCAATGTACGAAATCTTTGTCGAAAAGATGAAACAGAAGGCAGATTTAATTATTCCCTGGAGTGAAAGACACGAAGTGAGTACTAATGTGCTCGACTTTGCAATTGAACACCTCTTTCGCAAACACGTGGATCCCAACTAGATTTGGAAGGAATAAAGTGGTTTAAAGGTTTTAGGTAAACTACGCTTGTGCTGGGAGCTTTGGCGCAAGTGGTCAACCCGTTGTTGTTTCGTGGCACTTAACTGTAAGTGGCCCTGAAAGTACTGGTCCAATTCCTTATACGTCAAACCCATTTCCGCTTCGTCAGTCTGCCCGGCAAACAGACTAGCAGTAGGGGCTCGTTCAATGACCAATTCAGGAACATTGAAGTGCTGGGATAAAGCATAAACATCGCTCTTTAACAAGAACGCTAAGGGTGCCACATCACACGCCCCATCACCCCATTTAGTAAAGTAGCCCAAGCTGTACTCAATGAAGTTGCCAGTACCCAAAACTAAGTAGTTGTGCTTTTGGGCATGGGTGTATAGACAAGCCATTCGTAAACGTGCTTTGAGGTTGCCGGCTACCATTAACTCCTTTTGGGGATCAATGGTCAATGCTTGCAACATAGCGCGGTAAGGTGGTTCTAAATCCACCTGTTTATTGTTGAGTTGTAATTGTTCCACTAAGGCAGTTGTGGCCTTGTGATCTAACTCGGAGTTATTAATGTGCATTACCAAGGTTAAGTGGTTTTGCGGAAACAGTTTTTTAGTCATGGCGGCTACAACTGCCGAATCAACTCCACCTGACAATCCAACAACCACGCCCTGAGCGTGAGCTTCATTAACGTAATTGGTGAGTCAAGCTTCCAATTCGTTTAAATAGTTCAATAAATTTTTAGTCATTAATTACAAATTCCTGGCCAGCAAACACAATGACATCGCCTTTTTTGGCACCTGCCACCTTTAATTGGTCTTCTATGCCCACTTCTTTAATCTTATTACCTAAACGGCGAATGTTGTCAAGTGTAGTTTGGGGAATCTTGTCAAACCAATAATGTAAACGCTTGCATTCAACATGCCACCGCCCCAAGGCATCACGTTCAATGTTGAGTGGATCATGGTCGGTTTCACTGGCCTGTTCAAAGACATAGTGCTTCTCCATTTCCATGGGTAAATGAAACTTATTGGCACCGAATTGCGCTAGCGTTTTTTGGTGGAGCGCAAACACTTGGGCCACTAGATCACCCAGTTCTTGTTTGAGTGCACTAATTTTGAACAAGGGAATTCCTTTATTTTCGAGGTATTGTGCTAACTTTTGAAAACGGGTACCATCAAGGTCAACGTCTGTTTTATTGGCCACGACCAGCATCTTCTTTTTAACTAACAGCTGGGAGTACTTCGACAGTTCACGCATAATTTGTTCATACGCTTGACAAGGATCGTCATGATCCACGGGATCGAGTGAAATTAAGTGAATTAGGATTTCACAACGCTCAATGTGGCGCAGGAAATAGTGACCCAGACCACTACCCTCACTGGCGTTTTCAATTAACCCGGGAATATCAGCAAACACCAAACTTTGGTCGTTGTGTTTAACTACCCCCAATACGGGCACTAAGGTTGTAAAACGGTAGTTAGCAATCTTGGGTTTAGCGTTGGACAGTTTGGAAATTAAAGTGGACTTACCGGTGTTGGGAAAACCCACAATCCCCACATTAGCTAAATACTTTATTTCCAAGCTCACATGCAAACTTTGACCCTTGTCGCCGTTTTCATACAGATTGGGAGCGCGCATAATTGGACTTTTAAAAGCTGCGTTGCCCTTACCGCCCTTGCCACCGTAACAGAGAATAAAGCTCTGTTGGTCATGAACAAAGTCCATTAAGATCTGGTTAGTATCAAGGTCACGCACCGTAGTTCCCACTGGTACCTTAATAACCAAATCTTCCCCGTTTTTACCATGGGCTAAATCGGGTTTGCCATTGCCGCCACTTTCAGCAAACAGGTGTTTTTTATTCTTTAAAAAAAAGAGGGAATCGCAGTTGTGGTCGGCTTGCAACACAACGTTACCACCATTGCCGCCATTACCGCCACCAGGACCACCCTTGTCATAATGGGCTTCCCTGCGTCAAGCAATAATGCCATTGCCCCCGTTACCCGCACTAAAGCGGCACTCACAATAATCTGTTAATCCCATGGTTGAGCTCGCTTGGTTTTAATTTTCGTATTGCTTATGTTGGTTATTAAAACCAGGTTAGTAAGTTTATTAATTAAAAAGCAGCTTGACATGAGATTGTTCAAACAAAGAGCAGCTATCCCAGTGTTTCAAGTACGGGGAAACAGGCAACCATAAAGGGACCATGTACCAACATGCTTTTTTTGAAAGCTAACTTAATTTGGTTAAAAAATAACCACGGATTTAATTAAGTCCTTCGGTTTATCTTTCATTAAAAACAGGGCTTCTTCAATGTGTTCAAAACCCTTAAAGCGATGGGTAATCATTTTGGAAGGATCGAGTTTTTTGGTCATAATTAAACGTGCTAATTTTTCTAACCTTAACCGACCACCAAGCATAAGACCACCATGAATGGCCTTGTGAGCCATACCAACGCCCCATTCAACTCTAGGAATAGTTAAAAACTCACCAGCGCCAAAGTAATTAACGTTACCAATTTTGCCTCCTGGTCTTAAAACCTTAATCGCTGTTTCTAAGATAGTCGAACCACCACCAGAAATTAACACCTTTTCCACTCCTTCATTGTTGGTTCACTTCAAGATTTCATCCACAACTGAAACCTTTTTGAAATCAATGTAATCAGTCGCACCGTATGCTTTGGCAATTTCCACAATATCTGGTCTAGAATCCACCACAATAATGCGTCCAGCACCCTTTAAGGCTGCACCAGCGATCGCCATTAAACCAACTGGACCAGCACAAAAAACGACAACGCGACACCGTATTGGATGTCAGCTAGTTCATTGGCGTGAAAGCCTGTGGGAATCATGTCTGACAACATACATGCATCAGCTAAATCCATCCCTTCTGGCATCAATGCTAAGTTACCCTCAGCATCATTGACGTGAAAGTATTCAGCAAACATGCCATCTTTAACGTTGGAAAACTTCCAACCACCCAGCATGCCACCGGAGTGCATAGGGTATCCAGCTTGAGCATTAACCGAATTTCATTCTGGTGTAATGGCTGCCACTAAAACGCGATCACCAACCTTAAAGCTTTTGACCTCAGACCCCACTTCATCCACAACACCACAGCCCTCATGGCCTAAAACCATGTTGTGACGTTCACCAATGCCACCTTCCCAAACGGTGTGGACGTCTGAAGTACATGGTGCTACGGCCAGTGGTCGAATTATCGCATCATTGGGCCCACAAACTGGTCTTGGTTTTTCGATTCAACCAGTAGCGCCAATTTTTAACATTGCATATGCTTTCATAAAACTTTATTTACCTCTGCTGTTGCTTTAATAACTGAATTTTAAATAAACAAAAATTATTAAAAGCAAGCGTCTCAAACTATCAGGTAAGAACGCCTTCAAATGGCTTCAAAGAATGGTAAGCAGTTGTAGTTAAAAGCTGTGTTAATAAAACACTGGAGAAAATGGCAAATAAACTAATCCAGCACTAATACAAAAACTGACAATAAGTTATTAATCACTTGATTTAACGCGCTTTTTCTTGATGGTAACAACAACTGTTTTGCTCTTTTCTTTATCTCATTTCGTTTTAATGTCAGACATAGCCTGTTGGCTTTGTTGTTTGACTTTACCTGTGTGAACCAGTCCTTGTGCTACTACCATGGCATAGCGTGAACGTAGTAAAACTAAGTTAGTTAGTTTATTAATTACAAAGAAACTCAGCATTAACACTACCAACACTAACACAAAACGCCCAAAGATTACCCAAACGTTTCAAGCTTGAGGAAACATACCACTCATAAAGAGGCTAATCATCATCAAAGCAATTCCATAACAAAAGGGAATTGCCTCCACAATTAGGTATGGAGTTGTAAAGAATAAAAGCAGTAAAAGCAACGCATAAACTACTAAGGGCACTAGTAAGAACCACAGTAGTACTAAATCAAAAATGGGTTCTACTTCCCTACCCATTAAAGTACGGTTATTTGGGGCAATGACAAAGTTAAAACCTAAGGCATTAATTTCCCCTAATAACACCCACACAATGATGTAGTAGACAAAGACCAAAAAGAAGCTAAAGCTCCCGGTTAATCAAGCTGGAGTTTTGCGCTTTGCGGTTGATTGAAAAAACTGCTCCAAGTTAGTAAAGTTGTGCTGCTATTTGAAATTTAACAAAGCGATCGTTTTCGTAAAACTGGTAACGTAAAAGCCCTTTAATTACCCAGAGGATAATTGGCAGGCCATAGCTTTGTAGCTCCGCACAATTTTTGTGGTAAAAGCTATCATTGGGCGCTAAGAAACGACAGTTCTGGGTAATCTCATCTTTAGTTAAATGAACTAACTGCTTTTTACTGTTAAAGACAAAACTAGTTTTAAAGCTCTTTAACTTTTCGTTGAGGTACTTAATCGTGGAAAAGTGACTGGACAGCAAGATTAACTTTTCTTTAGCTAACTCAAAACCTTCTAGTAGTTGTGCTAACTGTTCTACTAGTACCTGGTTTTGTTGTTCTTCTTCGCCCTTAATAAAAAGCAAGAGAAAGCGAAACGGGGTTAAAAATTCCATTAACACCTCTTTTAAGGTCAAGATTGTCTGTTCTTGGACATTTACCTTAAACTGCTTCGCTAAGTTAATCTTTTTGAGATTAACTAGGTTAATATCACTCAGTTTATGGGTTTTATTTCCTACCTTATAGTTGTCTAAATTAGTGACTACTATTTGTTTATCTTTAGTGAGTTGCACTTCCATCCACAAACCATCAAAACCAAACAATTGTGCAGCAGCAAAGGCTAAATGAGTGTTTTCAGGAGCAATGGTGCTGTAACCACGGTGTGCAATTAAAAACTGTTTGCGCATAAATTAGTCTTCGCCAAATACCTCCTTAGCATCGGAATCGGAAAACTGATCTAATCATTCATCAGCATCAATCGAGTCCACATCAAAGCTTTCAAACACAGGGATATCCTTGGCTATGTCAATGTTCTTCGCATCTTCAGCGTTGGAACTGTGGAATACTAATCAGTTACCCTCACTGTCAAATTCCCCATCGTACCACTTTCATTCATTATCTTCATAGTAACCATAGTCTTCGTTGCCTATTAACTGCTCCCAGAACCGTGGTGCTTCACTAATCCTTGGTTGATGATTCTGATAGTGTTCATCAACTAAGACTCATTCACCGGTCTCATCATAATAACCGTATCAGATCCATTCTCCTTTACGGTTATATCTACCATAGATTTCATCACCTATTAGATCTGTGTAATCACTTTCACGTGCCCATTCAACACTAGCATCTGGTGTTCATATTTGGTTGTCATCAAAGTAACCAGCTCATACCCATTCCCCACTAGGTTCATAATGACCATAATTGCTATTACCAATCAATAGTTCTCAATCAAGTTGCTCTTCTTCAACGTCATCAACTACTTTTGTTGCTTCAGTTTCTGGTTGAAGTTCTGGAGCTTCAGATGTTGCCTCTACTTCCAAGGTTTCTGGCGTTACCTCCGCTTGAGCTTCTGGTTTAGTTTCAAAATCTGAATTCAAACTTACTTTTGGCTCTGATTGAACTTCGGCTTCGAATTTAGATTCAATTTCTGATTCAGGTTGAACTTCTACTTCTGTTTCAAAATTAGGTTCTGGTTCAGACTCGAAACTTGATTCTTGTGCTAGCTCTTGTTGAACTTCAGTTTCGGATTCGAAATTAGGTTCAATTTCTGGTTCAAGCTCTACTTCTGGTTCAAAATTAGGTTCTGGTTCAGTAGTTTCAATTGATTCTGGTTCAGGAATCGTTTCCAAATCAAAGTTAGGTTCTGGTTCAGGTTGAATTTCTACTTCTGGTTCAAAGCTAGGATCAGATTCAGTATTTAACTCTGAAGTTTCAGCGTCTGCTGTTAAAACAACTTCTTCAGCATTTGCTTCTGTTTGCGCTGTAGCTGTGGATACCCATTTGTTATCTTCATCGAAGTAACCGGTTCAAATCCAGTTTTGCTCTGCATCAAAGTAACCGTATTCACTGTTACCAATTAACGCCTCCCAATCCAGTTCAGCTTGGGCTTCAGAAGTTGCTTTTGACTCGGTTTCAATAGCTGGTTCGACTGCAACAGCTGTTGACTCTTGTTCAACTTGCGCTTCTTCAACGGTCTCTGCAGCTAATGTTGTTGAAGTTTCAACAATTGGTTCTACAGCAAGTGGTGCTTCTTCAACAACTGCTAATACTTCTGGTTCTACTGCTTTCTCAGTAACTGTAACAGTTTCAGTAACAACTGGTGCTGTTTCTAGTTGAACAGTTTCAAGCGGTTCTAAACTTACTGGTGTTTCTGTAAGTTCAACCGTTTCAACAACTGGAGTTGATGCAACATTAGGTTCATTTGATGTTGCACTTACAACAGTTGATTCAACAAACTCGTTTTCTAAAAGTTGTTCTTCAGATTCTACTGTTGGAATTACAACTGGTTCAAAAACTGGAGTTGATGCAAAAGTAGTTGTTTCTCTACTAGTAGTTGTGACAAACTCATTGACTAGCTCTTGCTCTTGCAACTCTATTTCTGCTACAGTTAGTGGTGCAGTGCTAGTGAGAAAATCAACTACATAATGAAACCCAGCTTGTTGGGGTTGACCGTTGTCAATCACCACCACCTCTTGAATCAGCTCGCTTTGTGGTAAGGCTGTGGTGGCTGTGGTGCCAACCGCCACTAATTCATCAATGAGGTCAGCTTCATTTAAAGCAATGTTAATTGCTTGAGAAGGGGCTTGACTAGCTGCAGCTGCTGTTTGGCTTGGTTTTTTGAAATCAAAACTTTCATTAACAAACTCAACCTTAATAATTGGTTCTTGAAACTGAATCGAACTTGATGCTGGTTGTGCTTGTACAGTAACCACTTGAGGTTGAGTGGTAGTTGTTGACGGTGCAACAAACGGCACTTCTACCATTTCCGTTTCCACTTCCGATTGGTCTAACAGTGGTGCGCTAACACTTAGTTGTTCTAGCTGAGTAGTTTGATTAAATACAGTGGTCTGCTCTTCTATGACTGGCTGTGATGTAGAAACGGTGACAGCAGCAGTTGGTGGTGATGTTTGGTCTACTACAGTTACAGTTGGTGGTGCTGGTAATTCAATTTGTACAACAGGTGTAGTTGTAGTGGCTTTTTCAACCATTGGCAAGGTAAAGCGCACTGGGTCAGGAATCCAATTGAGTTTGGTGTCAAAGAAACCCTTCCACACCCAAATGTTGTTTTGAATGTAACCATAGTTAGGATTACCAACCAGTGGTTTTCAAAAACAGCTATCAATCTGTTTGCGCTTAATAAAGGCAGTATGTTCTTGGGGCAGTTCCACTGTTTCTACCACTTCAAAGTAATACCAGTTTTGGTGTTGATCAAAGAAACCTAATCAAATTCAGGTGTGGTTCTTTCAAAAACCAAAACGAACATCACCAACAAAGATTTGTCAAAAGAGTTGTGCTTTTTCAAAAATCGATTCACGTTCTTGACTGTGGCGGGTGCTGGTTAGAACCTGATTATGTTCTAAAGCAACCGGTTTTAGTTCAAATTGTTCGGTTTCAACAAGCTCACTGTTGGCATAATGTGCCAGTTGATTTTGACTAATAGCAGTTTTGCTAACAGTCTCCTCTACCGCTTTATTATCATCCCGGTCATCGTTAATAACACTAGTACCAGCTGGTGGCACAATAAAAACACCACGCTTTTTCTCGTCAAAAAAGTCAATAGAAGATTCAACTATCTGTTTTTTGACTTTAGAAGTACCTCTGGTAGCGGTGCTAGCTCTGGTAGTTTTATTCTTCTTAAGTACATTGGTTTTGATCTCCTCTAAGAAGGGCTTCTTGAGTAAATCATCGATGCTAACATCCTTATTTTTAGCTTTAGCAGTTTTCTCTTTAGACTGTTCTAAATACTTTTTGTACTGTAATGTAGTGTTCGATGGGTTCTGTTTTTTTATCGTTTTTGGCATTAACAAACATGCTTAGCACTAAATGCCTTGAGGCGTTTTGCGATTAATAAAATTTTAATTTTTAATATTAATATAACGAACTTTATAGCGCTAGCGACACTAAATTAAAGAGCCATAAGGTGACTTTTTTTGTTTAGGGTCATAAGTCCTTACAAACAATTCCACAAAGACATTGCAGTCATAGTACTCCAGTAGTTTTTGACGCATTTGCATCCCGATCTTTTTCACCATTTGGGCATTACGGCCAATAATAATTTTTTTTTGACTAAGTTTAGGCACACTAATGGAAAGGTGTAGCTTCAACAGGTTTTTAGCTTGATCAAAACTCTTATCGACAATCTCTAGCCTTACTACATGGGGAATTTCTTCGCTGCAGTACTTAATGATCTGTTCGCGCAGCGCTTCTAAAATCTTAAAGTTATCAGTGTCAGCATCTAAAAATTCAACATCTTTACGAAACTCATTTGCTTGTAGTTTCACTTCGTTAAACTGGTTTCAAAAAGAAGTGGCATCAAAATGAAGCAGATCAGTTTCCAGTACTGCTTGCGGTTTAAAATCGAGCACAATTGCTTGCTTGTCCTCCGCTTGCAAAACAACATCAAAGAAGTTATTAATTAACAAAACACGAGTTAAATCACGGTAACGCTTTAACGGTTGTAATTGCGTTTGTAACAAGGGTAACTTTTGCTGTTGATCACTGCGCACCACTAACAACAAGACATCTATCCCACTTAAAGCCTTGCGAATCTCTTGACTAATTAACTCATAGGAGCTGTGACGTTTTTCACTAAAGCCAGGAACATCAATGAAGGTAATGTTTTGCTTTTCATGGGTAATAGTATTAGTGCTAACCGCCAACAACGTAGTGTTGTTCATAGGTGAAACCAACAAGTTATTCGCTTTGTGTAATTGGTTAACGAGAGTTGATTTACCAGCGTTGGTTAGACCTAAAACACCAATTCTTATCGATTCCATAGTTTTAAAACGGTTTCATGAATTTCATCTTGAAAAAACATCGTTACACTTTCAAAGGCCAGACTTTGCTCATGGTCAAACTCAAACAGGTGTAGCAAACCATGGATTAAGTTGCGTGTTAGTAAAAACCAAGGTGTGCAACCAAACTGCTTGGCCTGCTTAAAGATGTGTTTGGGACACAAAAAGATTTCACCAATGGCTGGTGAAAACCCCGCCTCATTTTCGTTGTAACAGATGCTAATCACATCAGTTACCCCTTTGCGGTTGAGAAACTGCCGGTTTAAGCGCTTCATCTTTCATGGTGAAACAATCATAAGCGCAAATTCTGGTTGAAAAGAAAGCTGCAAGCTGTCTTTAATAATTTGCAAACAAAGCTCGATGCCAGCTTCAAAGTTTTTGCCAAAAAAGCGCTTAAACAAGTAGTTAGAGTTAATACTAAAAGACGGTTTCAAACAGCGCTTTGGACGGTAAAAGAATCGTAATATTATACCAACCTTGTTTTAAGTTAATACTTAAATTAACCGCTTGAAAAAGCTGTTGCTGGTTTTCATTTTCTAGCTGCTCTAGGGAAATTTTCTTTTTGTAAAGTCGAAAAGCTTTCTTATCTTCCTTTTTAGTTGTTTCTGCTTGAAAATTAGTTCAAGATACCTGGTTAAACTGGGCTTCATCCTGCTCGTTTTTAAAAACTAAAAAGGTTTGTTCAGACAATTTTGCCCATAAGCTTGTAGTTAGGTATCGTTGTACCTTAACCGTAAATAAGATGGCAACGGCTGTTAAGAATAAAACAAAGAAAGCTAGAGTTAGGCCAATAATTTTTTGCTTCATATTATGTCTGCTGTAAATAAGGACTGAGGTTAATTAGGTCTTGCTCACTGTTTACAAAGCGTAAATCGTGTTCGACCATCAGAACAAAGTTGTTATAGGTCAACGGTTTGATCAAAAGCTGATACACGCGCGGTTTGAGCTCCGTGGCAATGGCGTTGAGTGTTTCATCTAACAAAATCACCTTGTCTTTGAGGTTTTGTAATAAGAACAGTTGCAAGATCTGTTTTTGTCCTGAACTTAACTTAATCTGGTTGTGGATGCCGGTGTCGGCTAACAGCTGGTATACTTGGGGATTTTTACAGTTTAACAAGGCTTCACTGTAACCAAAGTCAGTAATGTCGTTGTAGGCCGTTAACTGCCCCGTTAAATAGTAAACGTTGTGAAACAGTTGGCTTGCACTAAAATCCTTTAGGTCTAATTCATCCAACTTAATTTGACCCTGGTATGACAAACCTCGACCAGTCAGAATTTTCAGCAAGGTACTTTTACCGCTACCATTCTCACCACTAATAACCATGCCATTAGTTAAATTCAGGTTGAGGTTAGCCAAATCGAGATTTTGGTTCAGAGTGATACTACAGTTGTTTAAGTTAATCACTTTGGGACACTTCCAACTGACCTTGAAATTGCAATCATTAACCTTGTCTTCAAACAAGGTAACTAGTTTGTCCTTACAGAACTGGTACAGGTTAACTGCTGCACCAAACTGGATGATTCTCGTGGCATAAGCGGAAAACAATGCCTGAATGGAAAAGATATAAAAGAGAAAGGCTAAGGTGTAGCGCTGTTCAATAATGCCAATAATGCCCAAGGCCAAAATTAAAAAGTCAAATACCTGTTGGGCAAAACTTTTAACACCATCACTCGCTAAGTGGTTCACTTCCTTTTGATTACTAATGTTTTGGTTTTTAATAAGTGCTTTGCGCAACTGCATCATTAAGTAATTGAAGCGCTTGCCGTTTTGTTCATCACGCAAATTGCCATCTAACTGCAAACTGACCTTGTTTTGTAACTCCATTTGCGGAATTGCTTGCTTCGTAATCCGTTTAGTGAAAAAGAAGTCATAGAGAAAAATGGCACAATTCACCACAATTTGGACAATTGCTATCCATAAGAACTCCATTTGGTTAATACCAATTAAGGTGCCAATAATTAAAGCTACTGTACAGCTAATAATTAAGTTAGGAATATGGACATTAAAGAAGGACAACACTTCCTTGAGGTAAAACTGACGCTCGGTTAATTGGTTAGGTGATTTATTGCTAAAGTAGACAAAACTCTTGGTTTGTAATGTTTGCATAATTCAACCCAAATAGCCCCGATACTGCTTTCAAAACAAAACATTACGCACCATTTGCAACAAGGCATACAAAAGTAAGTACAAGCCTTTTAAAACTAAAAAGAAAACCACAAAAACGACAATGTTAACTGATGTCCCAAAGTCAACGGTGTTGGCAATCATCGTTTTAGAGGCCGTTGCTAAAAGTGTTGAAATGCCAATAATGATTAGTTCAATGAAGACATAGAAAATAATCAGGTTTAAATCAAAGAAGTGCTTAAAGAAATTGCGCTTATTAATGGGCGGAATTTCTTTAAATGATTTTTGCACTGTAGCGGCATAACCAGTTCACTGTTTCTTTAATTCTTCATCATTTAAAAGATATTTCCCTTTAGCGGGGTCAAATACTTCCCAGAGATTATCTTGTTTTTTATGCACCACCACAAAATGCTTAGGGAAGTGGACAATAAAGTATTTACCCTCTAGTTGTTTGAGCTCTTCTAGGGTTAGCTGGTAACTAGCAATTTCGAGACCAAACTTCTTGCCATAGGTCTCTAGTTCAAAGAAACTCAAACCACTAGCTGGTAGGTTAATTTGTTCCAACAGTTCATCATGGGCATACTTATCCTCATGCAGTTCATTGGCTAACATGCCAAGCACACAAATGCCACATTCGTTGGGTTGTTCTTGATAAATAATTTTCATGGGGCTTTATCACTTACATAAATGACCCAATGAGGCTAAGGTGCCAATAAAAAAAGGGCTGTACTACTACAACCCTTCTTTTATCTGCTTATTGAAAGCCTGTTTATTCCTTTGCTTGCTGCGCAGCACGTTCGTATAAAGTACGTACTAAAACCCCTTGACCAAGATCTTGAATCGAAGCTGTGGATGCAATATCACAGTGGATCAAAGGTACCCCTTCCCTAAATTCAGCTAAGAAACATGCCGCCCGGGATGAACCTGCTCCCTTAACACTAGTGGAGTTTTGTAAGTCCGCTAGTTTTGATTCCAATGGTTTTAGGTAATCCGGGTGCATTGGCAAGCGTCATACCGGTTCGCCTGCACAGCAAGCTGCTTTTTTAAAGGCATCCCATGCCATGTCACAGGTACTAAAGATCCCGGTGTAAGTGGTACTTAAGATGTAAGACATTAAACCGGTTAAGGTAGCGACATCAATAATTTGGGTAGCTTTAAGATCCTTAGCAGCATAGCTAATGGCATCGGCTAACACCAAGCGACCTTCAGCATCGGTATTGTCAATTTCAACCGTTTTACCATTGTAAGCGGTTTGGATGTCATCAGGGCGTTGTGCGTGGGGACCTGGTAGGTTTTCTGTTAAAGCAGCTACTGCTACTACATTGGTTTTCACCTTGTTTTTCGCTAAGGCCAACACGGTGGAACACACAATAGCTGCCCCACTCATGTCGTACTTCATACCACGCATGTAGTCACCGGTCTTAATGTTCATCCCACCCGAATCATAGGTAATGCCCTTACCAACTAAAGCTAAAGTTTCACTGCTCTTCTTATTATTGTTATAGGAAATTACTAGTAAACGCGCTTCACGTTCCGAACCCTTGTTAACCCCTAACAGTAAGCCCATCTTCTTTTTGATGAGATCAGCTTGCTTTAAAACGGTAATCTTAACACCTAAACCAGTAGCCGCCTTTTCAAAGCGCTTCACAAATTCCTCTGGGTAGAGTTGGTCAGAGGGGGTGTCTTGGAGTTGACGCGCCATGTTGACATAGTTACCCACTACCTCGTATTCTTTAATGAGGCTCGTGTGTTCGCTGTCACACTTCACTGCTACTTCCACATTCTTTTGTGGTTCGGTCTTCATGGTGAAAGGTGTTGTTTCACAGAAGGTAATGCTAGCTACCACTACCTTTAATGCTGTTTTGTAACAGAGGTTTTCTGCTTTGTAGAGTTCCACAAAACTGTCTAAGTCCACGTTTAGTTGATTCTTTTGCGTTTTCAAAAAGGTAACTAGAGCGCGGTTGAAAGCGGGGAAATCAGTCGGAATTTTTACGCCAAATTCAATTTGGCCTTCAACACACTTAGTCTTACAGCAATCACTCTTGCCGTACTTATCGGACTTTTTAAAAACAACCGTCTTGCTGTCAAATAACTTATTTAATTTCATCTTTAATTTTTACCTATTCTTGGAAGCTACCTTCCTTGTTTTCCTTAATATCATACACAGCAACGCTGGTGGTAATTAAGGAACTTGCTACTGATGCAGCTTTTTCGAGTGCAGTTTTGGTTACCTTAGTAGGGTCAATAATACCGCTGCGGATCATGTCGACCTTCTTTTTGGTTTCAGCATCAAAACCAACACCATCAGCTTCACTGCGTAGTTCTGCAAATACTTTAACTGGGTTAACCCCGGAGTTTTCAATAATTTGGCGCACTGGTGCTTCTAGTGACTTACGCACAATTTCATAACCAACCAGGATTTCCTTGAGGTTTTCCGCACTAGTTTCTGCCTTGTACTTGTCAGCCAACTTACTGTCATTGAGGATAGTGGATACGTTAAGTAAAGCAATACCACCCCCGGAAATAATCCCTTCTTCAACAGCTGCCTTAGTCGAGTTTAAGGCATCTTCAATGCGGAGTTTTAACTCTTTTTGTGCTAGTTCCGTAGCACCACCAACCCGTACTACAGCTACCCCTTGACTGAGGTGGGCAATCCGTTCCTTTAACAGATCGGTATCGTACTTTTCGGTAGTTTGCTTTAAACGACCATTTAGAAGGTCAAGGTGTTTTTGGATCGTTTCCTTAGAACCCTTACCACCAATAACCGTGGTTTTTTCTTTGGCTACTTGCACACGGCGAGCTTCCCCTAAGTGGTTTACAGTCACATCCTTAAAGCCACCACCTAGTTCATTGTTGTAAGCTAAGGTGCCAGTACTAATGGCTAAATCTTCCAAAGCTGCCTTTTGGCGTTCCCCGTATTCGTTACATTTAACAGCAACTACATTAATGGTACCGCGAAGCTTGTTCACCGCTAAAGTAGTTACCACTTCTTCAGCAAAGTCAGGTGCCACGATTAACAGTGGGTTACCGTTTTCCATGCTACCTTCTAGTAACGGTAAGATTTCCTTAATGGTGTTAATCTTCATCGCACTGACCAAGATCTTCGGTTGGTCTAACACCACTTCCATTTTTTCTTGATCGCTAACCATGTAAGGGGAAGCGTAAGTACCTTTAAACTCAATCCCTTCAGTGGTTTCTAGGGTAGTGTTAATCGTCTTAGCATCATCGGTGGTAATGACACCGTTTTTACCAACTAAGGCCATAGCTTGAGCAATCAGCTTCCCAATTTCCTTGGAACCGGAAGAGATCGCTGCAACTTGCTCGATTTCTTCGTTGGTATTAATCTTTTTGGAACGCTTATCTAACTCAGTAATAATTAACTGACTAGCATCTTCAATCCCACGGCGGACATTAACTGGATTAGCGCCATTATTAACAGCTTCCACACCACGGTTAGTCATTTCCTGTGCCAAGATGGTAGCAGTGGTAGTACCGTCCCCAGCAATATCATTGGTAGATACAGCAGCTACCGAAATAACCTTAGCACCAATGTTTTCTAAAGGATCAGTTAGTTCAATTTCCTTAGCGATGGTCACTCCATCGTTAGTAATTAAAGGATTGGAAAACTTCCGACCTAAAATCACGTTTTGACCTTTAGGACCAACGGTTACCTTAACAGCGTCTGCTAATTTATTAATACCAGCTAGCAGTTTATTGCGGGCATTCTTGCCAAATACTAATTCCTTTGCCATAACTATTTTTTCTTAGCTCCTTTCTTTACAGTAGTAGTTTTTGTATTACCATGCTTTTCAAAAGCAAGGACATCTTCCAAGGAAATAATCTTGTACTTGGTACCTTCCTCTTCAAAGGAAATACCACTGTATTCCTTAAAGTAGATCGTGTCACCGATGCCAAAAGCATATTTCGGTTTTTCGCTTTTACCATATGCCGGTCCTGCACCTAAAGCAACGACAGTTCCCTTGTGGGCACTTTCATTTTTATCAGGGTTAGCTAGTGCTGTAATAATCCCTTTTTGTGACACTTCTTCTTTGTTTGATTCCACTAGTGAAACCAAAATGTTGTCATGAATTGGTGTTATCTTCATACCGTCAATATTCTACCAATTATTAACAATTAGCAGTTAAATGCTTAAAGTGCAAATAAAAATAATTTAGCAACACTACCGAAATAGTGCTGCTAAATTAGCGTGCCTGATTTATTTACTAGTAGATTAAGCGTTTATTGCCAATAAGGCACTCAACCTTGTATATGTGGTTAGTAATTAAAACTAATTAAGAGTTATTTTCTTTTGGAAGTGGCAGACTAGCATCAAGCACACCGAAAGCAATTAACATGACTACGGCCACTAACAACAATAAGCTACCGACAACAAAGAAAGCAACAAATAGATTTTTCGTTTTTGCATGAGCGGGACTACCCACTTCAGCGTATTTGTGCTTATGTAGGAAGATGCCACCCAACAAACAACTCAAGAAACCAATGATTAACAACAAAATAACTAAAAGGCTAATTCCACCAGCAATTTGGAAAATGTTACTCGTTATTCCTTCGGCTTCATTGAAACCAGGAGTTTTACCACTAGAATTCTGGGAACCAAGCATTTGCTTTTGGAAGAAACGCGTAATAAAAGGAAAGCTTAAAGCTAAATTTTGCAGCATGAAAAATATTTGTTTGGAAATAACAAATACTTAAAACAACTAAAAAACCAATGCTGACAGTAGTAACGTTAACGCTAGTTAATCTATCGTATCTTGGTTAGGAAATTTAATTGAAAAACTACCACAAACTTTTCAACACCCATTAAAGGCTTAAAAAGTTTCAACAATAAAAACTAAAAACTCTACGCCATCACTGGCATAACAGGAAAATTTAATTTTTAAGCCTAATGCTAATTTCTTTTAAACCCTAAAAAATTAATTGGACTCGCTAATGAAAACGGTTTTAACTCGATAAATCTAATGGTTTTATTACCTCTAAAGTGACCAAGCTTCTAATGGTTTTGAACTGTTTATTTCAATAAAAAATCACAGCATCTTTTAATGAGTGAAGTGATTTTTTTAAATGCTGTATGTTTAGTAAATTAAGTAGTTATCTAATACTCCAATCCCTTATACAACGGAAAACGTTCTAACAGTTTTAAAACCGCTTTTTTTACTTCTTTAATAACTTTCTTATTACCGTCAGATTTAATAATCTTGTCAATTAAGGCAGCAACATAAAGGAAGTGCTTTTCCTTAAAGCCACGAGTAGTCATGGCAGGTGTACCAATACGAATACCGGAAGGATTTATGGCAGGATTCTTATCGAAAGGAACCGTGTTCATGTTCAACACAATGTTGGCTTGTTGCAATCATTGTGAAACATCTTTACCCTTACCGACATCAAGCGAGAACAAGTGGGTATCAGTTCCGTTAGAAATGACGCGGTAACCTTGTTGTTTGAACCAACTAGCCATGCTCGCTGCATTGGTCTTAACGTTTTGGATGTACTGCTTGTATTTTGGTTGTAAAGCTTCTTTAAAACAAACATACTTAGCCGCAATTACGTGCTGTAAGGGACCACCTTGACAACCCGGAAAGACACCGCTGTCCAATTTGCGAATAATCGCTTCGTTATTGGACATAATTAACCCTCCCCGTGGACCGCGCAGTGTTTTGTGGGTAGTAGATGTAACCACATCAGTGTAAGGTAAGGGATTGGGGTGCAAGCCAGCTGCTACCAATCCCGCAATGTGGGCAATGTCCGCTAAAAGGTAAGCACCAACTTCTTTCGCAATTGCACTAAAGCGCTCAAAGTCAACGGTTCTCGAGTAATTGGAAAAACCGCAGATAATTAGTTTAGGTTTTACCTCCCGAGCAACACGTAGCACCTCATCATAGTCCAACATCTCGGTTTCGGGGTTGATGGTGTAGGAATAAGGCTGGTACTGTTTACCCGAAAAGTTAAATTTATTACCGTGAGTTAAGTGCCCACCACAGTTAAGGTCAAGCCCTAAAATGGCATCGCCAGGTTTAAGTAGAGCTAAGTACACGGCATAGTTAGCAGAAGAACCGGAGTGCGGTTGCACATTCGCTCACTTCGCACCAAATAATTCCTTACAGGTATTAATGGCTAAGCTCTCTGATTCATCAACTACCTCACAACCTTCATAAAAGCGTCTAGTAGGATAACCCTCAGCGTACTTATTGGTCAAAATAGAACCAGTGACCTCTAGGATGTCCCTACTCACATAGTTCTCTGATGCTATTAAACAAATGCAATCTCTTTGTCTTTGCAATTCCTTATTGAGGATTCTTCTAATCTTTGGTTCCATGGTCTTACTAATTTACTTTAATTTTGATCAACATATTGAAACTGTCGATTGTTACTCTTTTTAATTTGATCACAAAGATGGGTGTAGTGTTGTTTGGCAAATTGGGTATAGTAATGGGTAGTATTGCGGTTGCCAAAGATAAGATCATAACCAATGCTGCTCTAGTTCGGATTGAGGGAATTGCCCTCACCCTGAAAGCGCAGCATTTGGACTAAACCAACATCGTTACGAACAGCCTTCTTGGGATTAGTAATTACCGTAGCAAAATAGATTCCCACAATTTGACGCTTTTGGTTAATCACTAAAGAACTAGAAGTCCCTTTTCTTAGGTTAACCTGATCGATTCCTAAACCATGACCATACATCTGGTAGTTTTGCCCTAACCACTTATTCTTAATCTTGAATGACTTAGTGTGATCAAACGGTCAAGATAACTTACTACCAGAAAAGTGTTTTGTTTTAATGGTGGTAAGTTGATTGGTAGTCAAAGTAGGAATTTCGCGGTGGTGATCTACTGACCAAGGCTCTTTAAAAGTAACTTGAGCAACATCCTCTGGGCGAGTAGGTTTAACTTCAGTTTGGTTCAAGTTAAGTGCAGCAATATTGCTCTCTACTTGGGGATAACCTAAAGCAAAATAGCTATCTCGCTTAAAGCTTGCCATAGGTTGGTAAGCAAAGATTCCCACACTGTTACCTAACCGTTCATAGGTATTGATGGCGGGGCGCATAAAGTGGTCATAGATCTGTTTATCCATTTGGTTGTGTAAAAAGAGTGGAACTTCTATGATCGCAAAATCCAAATAGCTTTTTGCTAGTGCAGGATCATCTTCTGATGCACGAACTGGTGCTACCCACTGGTTACCGCGGTTTTGTAAGGGCACAAAGTCGCGTGCTACAAAGGCTGTTTTGGGAATAGTGGAAGTTTGTATTGACACAAAGGCGTTGCTTACATCAGTACGTAGCTTAAACAATTGAGGGTTAATATATTTCCCTAGACAAAATACCGTAGTTAAACTGTTGCCAATGCTAGCTTTATTAAACGGTGCATAGTCCTTCGGATTGCTCAAAGCAACGGCAATATGCAGGTTAGTCGCTAAATAAAGATAAAACGGTTCGCTCAGGTTATGTGTTCGCTTCGGTTTCTTTCAATCAATTAATCAACCAGTACCAAACACACGTCACTTGTTGTTGGCAAGCTGCATTGAAAAGCTCAAATCATACAGGTCGTGTAAAGCTTCTGACTTTTTGGGAATAGTATTAATGAGGTAATTAGTCTTTCACTCTTTACGTTTAACACCCCCCGCAGCTGTAACGGTTTTCAAAAGGAACTACAGCTATTTAGGGGAATTGCTACTAATAAAAGGGCAGGTAGCAGAGTTGACCTACGAACACGAAACGCAAATTAAACACTTAAGTTAGCTTAAGTCTAATATTTAAGTGAAAACAAGCCTTTTTGAATAAACACAGATAGTTGCAGAGGCAAAACTGACTTAAACATTCGCTTAAGTCAATTCAAGGAATAAGCAGTAAATGAGCGAATTATTTCTTTACTTCTTTTTTGAGTTTAGCCACAACCTTATGTTGTTTGCACGATAGTTTAAAGCAAACAAAAAACCACTAAGAAAAAACGAAAAAGAGGATGCTCAATTCCAATAACCACCAAACGGAGTGAAAGTGACCCAGACTAATACAAATAATGGAAATAGGCAACTCTGCCATGAAGAACACAATGAGCAAAACGTTGTATATGTCTTACAACTTCATCGCTCGGACTAGTGGTAAAACTTACCATGCTCCGGTTTTGGTAGTTGATTGTTCAGTTAACGAAGGTTGGTTGTGGATGAGGATTTGCCACAACTGCTTGGGGTGTTTGTGTACTGGGTATTGGGCTGCACAACGAACATTTGCAACTACTACCGGTCTGAGGTTGGTTTGGCTTTAGTTTCTGGTTTCACCACTATTGGTTATCTTTGTGGATTGCTGATTGAGTATCAGTTGGACTTGTGATCAAAAGGCCTTCAAGCCGCAAAAACTCTTCTTCTTGGTTAATGTTTTTGCGCCATGTTTCCAAGAATCCCAAAAAGACTAAGACAAAGATAAAGGCAATAACAACTAGGGCAATAACAAGGCTAATTGGCAGATAATATAGAAAAAACAGTGAAATAAAACCGAGTCAGATAATTAACGACACAACAAACAATCACAAATAACACTTGTGTAATTTTCGCGCTTTATTGAGATCACCAGTTTGAAGTTGATCAGTATTATTTTGTTCTTTGTTCTCCATTCAAAATTTTCCCTACATTATTTGTTATCTACTATCCTGTTTGAATTTCCAGATTTACGAACGCGTAATTTTAGCTAAGAGCTTTAAAAATAAGTCAAAAATGGAACTGTTAAACAGCTAAATTACAACAAAAAATAACCGTTACGAAACCATTGAACAGGACACATTTTTGCGATCAATAAACTTAATTAACTGGTCATTACTTTGTTGAATTTGCTCATACAAATGGGTGTGGTGTTGCTTGGCAAATTGGGTATAGTAATGGGTAGTATTGCGGTTGCCAAAGATTAAATCATAGGGCACACAGTTGTCCTTTACCGTCGCTGTAGTACTTTCATCAACTGGCAAGCGTAACAGTTGTACCAGAGTTTTTTCACCTGGTTTAGGGTCGGAATGACTAGCTACTGCAAAAAAGATGCCTGATATTTGTTGATCCTGATTGAAGACTAAACTACCAGAAGCGCCCCTGCGGATATACAAATCATTTAAGGCTAAGCCTTTACCATACTGTTCATAGGTTTGCTGATTAAACTTTTCGTCTAGTTGGTAATACTGGGCGGTCATACACGTGTGCTAGTGTATTGCCAGATGTTTGCCCCGTTTTATTCATTACCAAGGTTGGAATCTGACGCTATTTGTTCAAAGTTCAGAAGCTTTGGTTTTTAACTTTAATGCGGTCTTTAACCGGAACACTAGTTAAAGTGGCACCTGTTTGGTTAAAGTGAATCGCATCAATGTTACTCTCTAGTAAGGGATAACCAAGAGCAAAATAGGTGTCATTTTGTAAATCAGAAGATGACGTACTTGCAAACAAGTTTGTACTATCACCTAAGGCCTTATAGGTAGCAATGGCGGGTTTGATAAAGTTTTCCCATACTTGGTAATCCAACTTGTTGTTTAAGTACAAGGGTACTTCTAACACGGCAAAATCAGCATAAGGTTTTAAACCAACTTCCGGACAAATTTCATTTAAGGATTTCCAATGTTTATTGTCAAACCGATAATCAACAAAGTCCACCGCTGCATAAGCCATCTTTGGTAAAGCTGCTAGTTGGACATCAACAAATGCGTGTTTGACATTCGTGTACAGACCAAATAAACTGGGATCAGTGTATTTACCAATTCGAAAACTAGTGGTTAAATCTTGGGTATTATCGACTTGGTTATAAGGCGGGTAGTCGTGCGGGTTCTTTAAAGTGTCAGCGACATGGAAGTTAGTCGCTAAATAGGCAGTGAACTTATTTTCGTTTGTACTAGTATCCTTTCAATCAATTAACCAACCAGTCCCGTAAGTATTTCACCGTTGTGACGTTAAAGTTAACGAAAAGCTTAAGTTATAAAGCTGGTGGTAAAAGTCAGTTGGTTTGGGAACGGTATTAGTAGCCCACTTTGTTTGGTATTCAGCGCGCGGTACCTTTTCTAATTGCACAATCGTGGCACATCCACTAAGCGGTACGGCAAATAAACAAGACTAGCACTAACAGAAAAACGCCACATACACAAACCTAGACAATTTAATTTAAGTATTTTTTACCTTTGATTCGTTATCTACATATTTTAAACTAGGGTCTCGCGCAGATTTAATTTGCCCATAAAAGTGAGTGTTATTTTTTTGCAAACTGGGCATAGTATTTTTTGGTATTGCTATTACCAAAGATTAAATCGTAAGCAACACTGTCTTTTTCTTCATTAAGAACACTTCGCGGTTTTCAGCGTAACAGCTGCGCTAGACCCCATTCTTGTGTTTCAGTGATTCTACTAGCAAAGTAAATGGCCACAATCTGTCGCTTGTTATTTAAGACTAAGGAACCCGAAGCGCCACGACTTAAACTACCATTAGTGATCCCAATTCCCTTGCCATACATTTGGTATTCTTTGCCACGAAACTGGCGATTAAACGGAAAGTTCTTGGTGTTATCATGGTTTCAAGCCCAGGTGCCACCATTAAACTTGTCACTTTTAATCCTACCCAAATGCGGTCCTTCTTCGTTAATTGTGGAAACAAAGGGCTGATCATTGGGTATTTGGGCTGTTTCACCAGGCACTGTTTTTTCTTTCCCGGTTTGGTTTACAAATAAAGTTGGCAGCTTTTTCGCTATGTAAGGATAGCCCAGTACATAATAATGACTATGTTTAAACTGGTCTAAAGTTGGGTAGGCAAAAATGTTTAAGCTATCACCTAACTGTTTGTAAGCTCTAATTGCTGGTTGGATGAAATGATCAAAAATTTGTCGGTCAGATGAATTATTGAGAAATAAAGGTAGTTCTAATACCGCAGAGTCAGCATAAGGATGAACTTCATCTTTATTTGGCTTAATTTGTACCTTTCATTGCTGTTCTCTTTGTTTTTTATTCTTTTGATCTTTGGATAAAGTGTAGTTCACAAAGTCATGTGCAATGAAAGCAGTTTTAGGAAGTACCGTTGGTTGTGCTGCTACAAAAGCTTGGGTAGAGTTGGGTAAGCCTAATTTGCTTGGTGCGACAAATTGTTTAACATCAGTGTATTTGCCAATTCTAAAATCAACGGTTCGCACAAAATTGATATCAAATTGGTTGTAAGGCGGATGATCTTCTCGGTTTTTTAAGGCCTGTATGACGTGTAAATTAGTAGCTAAGTAAACAGTAAATTTATTAGCTTTTTTATCTTTCTTGTCAACTTCCTTCCAGTCAATTAACCAACCCGTTCCGTGAATGGAACCAAAATCTATTTGACTTTTATCTTTTTCGGTTTTTTCAATTCTGTTAAAAAAACCTAAAAAAAAGGTTAAATCATAGTTGGCATGATCGATTTCTGCACTACCAGGAATATTGTTAATGTAATACCCTTCGAATTTATCAGGTTTTCGAAAGGCTGAACAAGCACTTAAAACAGTTAGTGCCAATAATCCACTAAGAAAACCAAAACGCTTGAGCGCAACTCACATAAACGAGTAATTCTAATAAAGGGTTAAGGAGCCCTGTTTGCAGTGATATCCCAAAGGATTAAACCACAAAATTTTTAAGACTTTTGGTTTTTAACAAAGGTAAATTGTTGGTCGTTGCTTTGCTTAATTTGCTCAAACAAATGGGTTTTATGTTGTTTAGCAAATTGAGCGTAGTACTTTTTAGTGTCTTTGTTACCAAAGATTAAGTCATAGGCCACTGTGTTTGTGCCATCGTTATTATGGCCATTGGCTTTTCAACGCAGCAACTGAGCTACCCCTCAAGCAGTAGTGGTGTTACTAGTAGACGCAAAATAAATGCCAGTAATTTGGTGCTTATCGTTTAACACTAAAGCACCACTAGCGCCACTTGTAAAACCAGCATTGTCCAAAACAAATCCCTTACCGTATTGTTGGTAATTACGACTTTGAAAAGTTTGTTAAAACCGAAATACCCTACTGTTGTCATAGTTTCAAACCCAGTTACTGCCATTAAAGTCATCACCTTGATTTCTAATTAGGGTTGGCTTTGCTATTGTCGATCGCACAATAAGGGGTTGTTCGTGGTTACTTTGTTGTACTGTTTGTCCATTTATTGTTGTTTCTGTAGATGTATCTTTCTTAATTTGATTAACACTCAATACATTAACCTTGTTTTCCAAGAAGGGATAACCCGATAAATAATAGCGGTGGTTTTTGAGTTCAGTTAATGTTGGCTTGGCAAACAGCTTTAAGCTATTACCTAATTGCTCATAAGTTTTGATGGCTGGTTGGATAAAATGGTCAAACACCTTTTTATCAACTGGGTTATCTAAAAATAAGGTTAGCTCTAACACCGCAAAGTCAGCATAGGCAAGACTGTCTGATGTATTTGCTGTATTAGATTCCTTTTTATCATCAGCATCCTTTTGTTGAATTCACTCAAACGGTTGGGTTTCCGCTTGCTCCTTTTGCTTATCGTTTTCTCCATCCTTTGGTTGCTTCTTTTTATTTTCTGTATCATTTTGCTTCTTTTTGTCTTTTGGCAAAGTGTAGTTGACAAAGTCATGCGCTGTAAAAGCGGTTTTGGGAATTACTGTGGTTTGGACATTAACAAAGGCTTGAGCATTATTGGGCAATTTAACTTGACTAGGTTCGACAAAATCACGAACGTGAGTGTATTTGCCTATTCGAAAAGCGGTCGTTCAATCGGCACTGGTATCAGAGTTTTTATAAGGATCGTAATCCTGTTGGTTTTTTAAAACGGCTGCGACATGTAAGTTGGTGGCTAAATAAACCCGAAAGGGTTCATTTAACCCCCCCCGCTACCTTTTCAATCAATGAGCCAGCCAGTGCCAGAAAAGGCGCTAAAGTTATCTTGCCCATTGTTAGTAGTTTTATTAGTAAAAGTTAAAGCAAAGGTTAAATCATAGTTGGCACGGTGAATCGAACCACTACTAGGAATATTACTTACATAGAACTGATCCCACTTTTCTAGTGGCACTGCAGCACAAGCACTTAATACAGTTAAGGAAGCTATGCTAGCGAGAAAACCAAGGCGCTTGAAAGCCACAATCATAATGGTGTTAATTCTAATAAAGAGTTAATTAGCTACTTTTACAACTAGCCACTAGTCGATTCATTACCCCAAGTTATGCCTTTTAGTCTTTTCGACAAAGCGGAATTCATCATTGTTAATTTGCTTAATTTGCTCGTATAAATGGGTTTTGTGTTCTTTGACAAACTGAGCATAGTATTTTGTGGTATTTTTATTACCAAAGATTAAATCATAAGCCACACTTCTAGCTTCATCATTGTGATAAGTTTCTGGTTTTCAGCGTAATAACTGTGCTAAGCCTAATTCATTGTTAGCACCATCAACGCCAAAGTAAATGCCAGCGATTTGTTTCTTATCATTTAACAACAAAGAACCAGAAGATCCACCGCCTAAATCAAGATGACTAATGCCTATACTTTGGCCATAGATTCGGTACTTTTTGCCCGAAAACTCAAGATAAACCTTAAATTTCTGGGAGAGATCATAGTTCAAAGCTCAGGGACTACCAGTAAAATTATCGCCTTTATTCCTAATTAAAGTGGGTTTAACTTGATTAGTTGAGATAATAGCTGGTTTTTCATGGGATATTTGTGTATTTTCGCCTAAATATTCCTTTCTTTTTTCCCGTTTGGTTAAGAAATAGTGATGACACTTTATTTTTTAAAAAAGGATAACCTAACACATAATAGCGATTTTGTTTTAATTGATCCAGTGTGGGGTTAGCAAAAATGTTAAGGCTATCACCTAACTGTTTATAAGTGCGTATGGCTGGTTGAATAAAGTGATTAAAGATTTTGCGATCTACTTTACTTTTCGAAAATAAGGGAATTTCTAAAATTGCAAACTCAGCAAAAGGCTGAACATCAGAATTCTTAGTATGACTGTTTTTCACCCATTGTTGTTTTCTCTTTTCTTTATCTTTTTGTTCTTGAGGAAAACTGTAATCCACAAAGTCCCTAGCAGCAAAAGCGGTTTTGGGAATTACACTTGTTTGCACATTGACAAGAGCTTGCGCAGCATTGGGTAAACCAAATTGATTGGGTGAAACAAAATCACTAACCTTAGTGTATTTACCTAACCTAAAGGTAGTAGTTCAACCAGCATCATCTTTATTTTTATAAGGTTCATAATCTTGCGGATTTATTAATGATGCTGCTACGTGTAAGTTAGTTGCTAAATAAGCACGAAAGGGATCATTCTCTTTCTTTTTATCAGGTTCCTTTCAATCAATTAATCAACCAGTACCATATGTGACACTAAATTTGGCTTCACCGTTGCTAGTGATTCTATTAGTAAAAGTAAGCGAGAAGTTTAAGTCATAGTTGAATCGGTGGATTTCCGTACTACTAGGGATATTGTTAATGTAAAACTCTTGCCACTTATCAAAGGCTGCACAAGCACTTAAAAAAGTTAAAGAGAATATGCTAACAATAAAACCAAGACGCTTGAAAACAACCAACATCGTGGCTTAATTCTAACAAAGGGTTAAGGAGTTAATTTTTAAGCAAAATCCCAAAGGTTTTAACTACAAATTTTTAACACTTTTGGTTTTTGACGAAGGTAAATTGTTGATCATTGCTTCTATCAATTTGTTCGTATAAATGGGTTTTATGTTCTTTAGCAAACTGAGCATAGTATTTTGTGGTATTTTTATTGCCAAATATTAAATCGTAAGCAACACTGTCTTTTTCTTCATCATTATTTTTCGCTTGTCAACGTAATAACTGTGAGAAGCCTAATTCGTCTTTAGGGCCATCAACACCAAAATAAATACCAACAATCTGCTTCCTATCATTTAACAGCAAAGAACCAGAAGAGCCACTGCTTAAATTAAGGTCACTAATGACTATACTTTTGCCATAGATTTGGTATTTCTTACCTAAAAATTTATGATAAAGCCCCGATTTCTTGCTGAGATCATAGTTTGAAGTTCAAGTGTTGCCAGTAAAATTAGCGCCTTTATTCCTAATTAAAGTAGGCTTAGCTGGATCGGTTAAAACAACAAATGGTTTTTCATGAAGTATTTGGGTAGTTTTGTCCATATGTTCCTTTCTTTGTTCCGTTTGATTTAAAAACAATGATGCCGCTTTATTTCTTAAAAAAGGATAACCCAACAAGTAATAACGGTTATGTTTAGGTTGATCTAGGGTTTGATTAGCAAAGATATTTAAGCTATCACCTAATTGTTTATAGGCTCTAATGGCAGGTTGGATAAAGTGATTAAAAATTTTGCGATCAGTAGAACTGTCAGGAAACAAAAGGATTTCTAAGACTGCAAAATCAGCATAGGGATGAACATCAGTATCCTTAGTTTGACTGTTCTTCATCCACTGCTGCTCCTTTTGTTCTTTACCTTTTTGTTCTTGGGGCAAGGTGTAATCCACAAAATCGTGCGCAGTGAAAGCCGTTTTGGGAATTGTGCTTGTCTGCGCTGATACAAAGGCTTGTGCACCATTAGGTAATCCTATTTTCGTTTGGTGACACAAAGTCTTTCACGTTAGTATATTTGCCCAGTCTAAAATCCACCGTTCAACCCAATTCTGCATCAGGTTGACTGTAAGGTAAATAGTCTGCCTCATTCTTTAACCCTGCAGCTACATGAACATTAGTCGCTAAATAAACCCGGAATGGTTGGTATTGATTCCCCTCCTTTCAGTCAATCAACAAACCAGTACCATAAAAGGCAACGAAGTTTACTTCATTCTTCTTTTTAGTCTGAATTCTGTTGGTAAAACCTAAAGAGAAGGTCAAGTCATAGTTAATCCGGTGTAATTCAGCACTACTAGGAATATTGTTAATGTAAAACTCACCAGATTTATCAGATAGTGAAATTGTTGCACAAGCGTTGAGCACAGTTAATGTAGCTATGCTAGCTAGAAAACTAAACCGCTTTAAGGCAATTACCATGGTTGCTAATTCTAATAAAGAGTTAATCTGGCACTTTTGGGATTAAATGCCAGCTGATTAATTGCCTAAATTATTAGTTGTCTCTTGTGTTTCCACAAAGCGGAATTGTTCGTCTTTGCTTTCTTTAATCTTCTGATACAAATGAGTTTGATGATCTTTAGCAAACTTAGCGTAATAATTCTTGGTGTTCTTATCGCCAAAGATCAAGTCATAGCTACCCTTAGAAATACTTGAACCTTTATCGTTCTGTGGAGTACGTAAGATTTGGCTCATCCCAATGTCATTGCGTGCAAGACTACCAGATTGGTAAATTTCCAACACCCCAAAGTAAATGCTGTTCAATTTATTGTCTTTACCAATTAAAGGACTACCCGAAGAGCCACCCGGAAAATTAGTGTCATTCAACATTAAGCCATAGCCGTATTGTTGGAACCGCTTTTCGTGGTACTTCAAGGTAAAGTTGCTAAAGGAAGCAAAGTTCACCACACCTTTGTCTTGGATCACTACCCCATTAAGCCAGGACTCGTTTTGTTGGTTGGCAAAGAGTTGACTACCAGTGCTTTCATTGTAAGCACGGTAATTGTTAGTTAGGACGGGAATCCTGTCTAACTTATTAATATAGCTTTGATCTTTTGTAAATCACTGGCTCTTTTGGTTATGATCCGAATTGTTGTGATCAAAGCTCTTTTGTTGCGCTAATAAAAGGTTAGCGGTATTGTTTCCTTTATTAATCACTGGATAACCTAACAAGTAGTAGTTTTGTTCGATGAACTGGTTGAGTGGTTTGGCACTAAACAGTTCCAAAGTATCACCTAAAGCCTTGTAAGCCTTAATTGCTGGTTTGACAAAGTCATGAAGAATTTGCTGATCGCGTTGATTTTTAAGGTTTAGGTTAACTTCAATCACGGCAAAGTCAGCATAGTTGCCAGGAAGACTTGTTGTGATGTTGTTATAACTGTAACTATTCATATCAACAAAGTCAGTAGCAGTGTAAGCCGTTTTGGGAATATTGGAGGCCTGAATCGAAACCAAGGCTTTATCAGTTTGATTGGCTGATTGATTTTCTTCTTTAGAAATGTACTGCTTTAACTGCTCACCTTCTAAGTATTTACCCAAAGAAAAGAATTCAGTTTTAGTATTCTCAGTAAAATCTTGCTTGTTCTGATCTTTTTTGTAGGGTTCGTAATCCTGATCATTCCTTAAAGCATCAGCTACGTGTAAGTTAGTGGCTAAATAAACGGTGAATTGATCATCCTTTAGAAGGGAGTTATCAATGTTGCTTGTAATGCTAGGGACAAAACTGCCATCAATACCTAATGCCTTTTCATCACCCTTCCAGTCAAATAACCAGCCGGTGCCATAGATATCGTTAGACTTATTGGTAAAGTTCAACGAAAAAGTTAGATCATAGTTATGCTTAAAAACATCACTTTCACTAGGTACCTTATTGGTTTGGAAGTGAAAGCCACGGTTAACAGGCACTACCTCTGCGGTTGGTTTAGGTACATCAGTTGTAGGTGTGGTAGGTGTAATGGGACCACTTTCTTCAATGTTATTGGGTTCAAAGTTTGGCTGGGTTGCAGCACAGGCACTAAGAATAGTGCTGGTACCCAGCAGTGTTAAGAAACCCAATAACTTGAATTGCAGTCTCATAGACTAACTATTTTAATACAGATAGCCAAGGAGCTAAACCATTATAGCCCGGTTTATCTAGTAAATAAAGAGACACTTAAACCATCAATGATTAATTGAATCAAGGATCTTTTTCGATATAACGGAACTGTTCATCAGTACTATTTTGAATTTCACTATACAGGTGGGTTTTATTTCGTTTAGCAAACAAAGCATAATAGTCTCTATTATTACTATCACCAAAGTATAAGGTCGTAACTGTATTTAACTAAACTTACCCCATTAACATCTTTAGGAACAGGCAATAGCTGGGCATAGCCAACACTATCTGATTCAGTAGCAGCTGAATAAATACTGCTAATCTTTTTGTCTTGGTTAAACACTAAACTGCCACTCGATCCACCTAGTAAATCAGTATCAGTTAGAGCTAAGCCATAGCCGTGGTTTTTATATTCATATCCATGATAATCAGTGATAGCACTTTTAAAAGTACCAAAATCAACAATTCCTTGATTTAAGGAATATTCATTAAGGAAGCCGTTACTGTAATCTGTGTGCACTTTACTACCAGCAACTGGTTGACCTTGCCTTAAGTTTTTGGTAAGTGTTGGCATAGAACCATAAAAGTCATCATGGTTAAAACGTTGTTCTGTTTTGGTTCACTCAAACAACTTAGCATCATCAGTCCATAAAGTTGGATAACCCAAAAGGTAATGGGTATCGTTTTGGTATTGGGTTAATGGTTAACTGGCGAATATACCAGTACTGTCACCCAATGCTATATAAGCTTTCATCGCCGAATCAATAAAGTGATCTTTAATCTGCTTATCTTGCTTATTACTTAAATCTAGATTGATTCCAATACAGCAAAGTCAGCGGCAGGTAATCTATATTCCCCCAAATCATTAATAAAAAGTGTTGTCGTGTAAGCAGTTTTTGGCAATTTTGAAGTTTCAATCCGCACTAAGCTAGGTTCATGCATAGCATCAGGAACTATTTGCTTTATTTCATTACCATCAACGAACTTACCTAAAGTGAAAGATTCAGTTGGATCGTCAAAGTAATCAAATTGATTGAAAAACTCATAATCATTCACATTTCTTAAATTATCAGCTACATGTAAGTTAGTAGCTATGTAAGCAGTGAATTTATTGGGCTGATTATTATCCTTTCAATCAATGAGCCAACCAGTACCGTAAGTAGCTGTATATGGATCTTGAAACTGGAGCGTAAAACTTAGATCATAAAAGGGACGAAAGGGATCATCCTTTTTTGGAATGAAGTTAGTATTGAACTTTAAGTCAGAGGTGTATTCACCTAAAGCAGGGTCTGGTTTTAATGCTTGCTTGTCTTCTGGTTGAACTGCACTAACACTTTTGTTGTTTACAGGCACAGCCGGTGCACAAGCAGTAAAAACTCCTTGAACTAGTAAAAGTGTTAAAAAGACAAAACCTTTTAACCTATATCTAAGAAAAAGCGGGGTTGCTTTAGTAATAGGCAAATTAAAAAAACTTAAACAAATACGAAAAATTAATGAAAAAGCTAACTAATGGAGCAATGCTCGATTATATAAGCTTGCTTTAGTGTGGGTTGGTTAAGTTGGTCGATTTAACACAAAGCTAATGAAAGTATGCTTAGCTTTTTACCGGTGTTTAACTTAATTGAATTGTTTGTCTACCACATAACGGTACTGTTCATCAGTACTTTTAAGGATTTCACTGTACAAGTGAGTTTGCTGTTTTTTAGCAAACATCGCATAGTAACGCTTAGTGTTGCTATCACCAAAGATGAGGTCATAGCTTTGTGAAACTACACTTATACCGTTGACATCCCTAGGAGTTCGTAATAACTGAGCATAGCCAACACTATCTGATTCAGTAGCAGCTGAATAAATACTGCTAATCTTTTTGTCTTGGTTAAACACTAAACTGCCACTCGATCCACCTGGCAAGTTAGTGTCGGTTAAGGTTAAACCGTAACCCTGTTGACGGTAATCGTAATCGTGGTAATTAATGTTAGCACCCAAAAAGGTACTAAAACGCACAATACCTTGATCCATTGAGTCCTTATCGAGTAACTCATTGCTGTATTCGATGTGTGTCTTACTACCAGCGTTTGGATCGCCCTGCCTTAGGTTTTTGGTTAGTCTAGCCATATTACTGTAAAAATAACCATAGCTAAAGCGCTCTGCCCCTTGTTTGCATTCCCACAGTTGTGTATGATCAGTAGGTACAACCGGATAGCCCAAGAGGTAATGGGTATCGTTTTGATATTCAGTTAATGGTTTGGTGGCAAACAAACCGGTACTATCACCTAATTGTTTATAAGCCTTGATCGCTGGATCGATGAAATGATCTTTAATTTGCTGATCCTGCTGGTTGTTTAAATCCAGTTCTAACTCTAATACGGCAAAGTCAGCGGCAGGGATTTTGTAATCAAAATAATCCCAGTAATATTTTTGGTCGTTAAATAAATAAGGAAAACTATAATACTCGCTTTCAATAAAACTTGTAGCTGTGTAAGCTGTTTTGGGTAGCTGTGAAGTTGCAATACTGACCATGCTAGATTCACTAAAGGCATCTAGTGCCATCTGCTTAACTTCAATCGCATCGACAAACTTACCTAAAGCAAATGATTCGGTTGGTGCCTCTAGATCAAAATAACTGGGGCGGTATTTAAAAGGATCGTAATCATTAACGTTCCTTAAGCTGTTAGCCACATGTAAATTGGTCGCTAGATAAGCGGTAAATTTGTCTTGGGAATTATGATCTTTTCAATCAATTAGTCAGCCCGTACCAAAAGCTAAACTTGCTTGGTCTTGAAACCTAAGCGTAAAACTTAAATCATAAAAGGGACGAAAAGGATCATCCGCTTTAGGAATAAAGTTAGTGTTGAACTTTAAGCCAGGGGTATATTTACCCGAAGCTGGATCTGGTTTTAATGCTTGCTTGTCTTTTGGTTGAACTGCACTAACAACTTTGTGATTGACAGAAGTAACCGGAGCACAAGCAGTAAAAGCAGTCTGTACCAGGATAATTGTTAAAAAGGAAATACCTTTTCACCTAAACTTGGAAAAGAGTGGAGTTATTTTCAGTTAGGACAAATTTCAAACAAATACTTAAAAATACACGAAATACCAATAAAACAGCGAAGCTAATTTAACAGTGCCCAATTATATAAGTTTGGCATAAATGTACTTAGGTTAAGCTGGAAAATTAACCCTAGCCCTCATTAAGTTGGCTTAGCTTTTAAATGGTGTTTGACTTGTTACAGTTTGATCGTTTTCGACAAAGCGGAACTGTTCATCGTTACTTTGAAGAATTTGATGGTACAAGTGGGTTTTGTGCTCCTTAGCAAATTGAGCATAGTATTTCTTGGTGTTGCTATCACCAAAGATTAAATCGTAGTTTTTGGACTTGTCTTTGTTTTGCGTAGCGCGGAGAATTTGCGATACACCGTAACTTTTACTTGGTAAAGCCGCAAAGTAAATACTGTTAATCTGTTTGTTTTGGTTAAATAACGGACTACCACTGGAACCACCCGGGAAGTCAGTGTCTTCTAACAATAAACCATAACCATGGTGTTGGTATTGCTTTTCGTGATAGTTAAAAACGTAATTTAAATACTTGCTAAAGTCCATTACGCCTTTAGGGTCATTTTGTGCAAAGGGTAAATAGTTAATTTGATTTTGTACCAAACGACTACCTGGAATTTCATCATCTTTCCGGATGTTACGCGTTATGCGCGAAACTTCCTTATTACTATTTTCATAGAGTGGTGAACTATAACCCTTCTGTGGTAAGTTTCAGTAAGACGTCTTTTCACGTTTAAGTAACGGATAACCCAATAGATAATGGGTATCATCAACATACTGATCTAATTGCAGGTTGGAAAAGAGACCCAAACTGTTACCTAGCTTTTCGTAAGCAGTTACCGCTGGATTTATAAAACTGTCAAAGATTTGCTGGTCACGATTGTTGGCTAAATTTAACTCTAATTCCAACACCGCAAAGTCAGCGTAACTTACGGCATTTTGGAATAAGTCCAACTCATAAACTGTGTTACTCACTATGTGATTGTATTTGTACTGAGCAGTTTTGATAAAGTCAGTGGCAGTGTAAGCAGTTTTTGGTAACTTCGATGTTTGAATTGAAACTAATGCATCACTATTCCGGTTATTGAGGTGCTTAAGATTTTGGTACTCTGGAGTTAACTTTTGCACAGTTTGGGCATCAATGTATTTACCCAAAGAAAATTCAGTGGTGATATCGCCAGGTAAAAACTCCTTCTGATTACCATCCTTGTTGTAAGGCTTGTAATCGTCTTTATTTCGTAAGCTATCAGCTACATGGAGGTTGGTAGCTAAGTAAGCAGTAAATTTGTTGTCCTGCTTAACATCCTTCCAGTCAATTAACCAACCTGTACCATAAGCTTCTTTTCAACTAGTACCAAACTTTAAGGCAAAGCTAAGATCATAGCCCGGCCGATACTTAGTATTTACTTTAGGAATAAAGTTAGTGTTGAACTTAAAACCAGGACTGTAAACACCCTTGGAGCTGTCAGGCTTAATTTGCACCTCGGCATTCTGCACAGGTGGTGTAGCAACAGCATCGGGCTCTATAACTGGTTCAGCCTCTGGTTTGGGGATAGTTTGGCTCAATGGTGGTTGAGGTTCAAAAATCACAAACTTATCACTTTCATTTTTGTGATTAGTGTTAGCTACTGTTAGCGTAGCAGAGCAAGCAGTTAGAAATGCTTGACTGGCGAACAGTGTGAGAAAACCAAAACCCTTGAGTTTAAACCCCATAGTGTGCTTTAATTTTAAAAAAGAAAGTTTGAAAGGTGAACCCTTAATTAAAGGTGAACTTAGTGCAGTACTAAAGTCGCACTTCTTCTAAAGGTCTGTTTTACTTTTTCGTTTTAGCACAATCACAGTCCATTTTGAGCTGAGTGTTACTAAAACGATTGGCAAAATAATCAGCCACAAATTTAACCATTGCTACAAAACTCAAGATAGCAAAGGCAAAGCCAATTAGTAAAAACACTGCTCCTAAGAAGTACAAGAAACCGGTGGATACTGGAGCATAGTTAAAGCAAACATAAGCAAAAATAAAACTAAGAAAAGTAATAAAGCTAAAAACAATCACCCAGATTAAGGTGCGCTTGGTGTTGTGCGGCGTTGTTGGCAACATGTTGCAAATTTCCGCTTTTAACTTCTTAAAAGAACACTTTTTAAACCACTGCTTTTTAGTGGTAGTGCTTTCTTTTTTGTTCATAGGTAATATTTAATGTCTTAGTTTCAATTTTAGTTGATGTTGTCAAACTTACCGCTAAAGCCTAAACACAAACTTACATAGCTTATTTAAATTTAAAGAAGGTTGTTGCTGTAGTTTTGAATAAAAATGCCAACCCGAATGATGGCCTTTTCATACTAAAAGAAGGAGCCTAAAAAAGAAGGATAATTCTGCAAACTGCAAAAGAGTTATAAGAATATCTTTAAACTAACTAGCAGAATTAGCCTTTGGTTTAAGCTTCTTAAAACTGACAAAGTAGAGAAAACTCGCTACCATACACACAAAACCAAAGGCTGCTAACACAAAGGTTAAAACAGAAAGAAAAGTTTTGACCGCAAATGGAAAATGCACAATCTCAAAGGGTTTCAAGTCAACTCCATTAGCATTGACATTAACCTGATACCCGTTAAAAAGTACTAATACACCAAGCGCTAAAGCTGCTATAAACAACAGTAAACCAACTGTTAAAAAAATAATGCTGTTGCATTTATTCCTATTTTTCCCTCTCATAAGCAATGATCTTACCGACACGTTTACTATGGCGCCCACCCTCAAACTGCGCCTGGAGAAATTCATCTACTATTTTAATGTTTTCTTCAGGAACCACAAAGCGGCTAGACAAACACAGCACATTGGCGTCATCATGTTGGCGTGCCAATGCTGCTGTTTTACTGTCTACCACTAAAGCAGCTAAGATACCCTTAGCCTTGTTAGCGGCCATACAAACACCCACACCAGTACCACAAATAAGGATGCCTAAACTATTAGGATTAGCTTGCATTGTTTGCGCTAACAAAAAGGCATAGTCCGGGTAGTCATCAAGTGGATCTAGCTCTGTAGGACCTAAATCAATCACTTGCAACTTTTGTTGTTCTAAGTGTTTTATCACTAAGCTTTTGAGCTCTAAACCAGTGTGGTCAGAAGCAATGTAAATTGGATGGTTCATTTGCATTAGTTTAAATCAAGGTGGTGTTGTTTAGCAGATGATTTAATTTTACCCAACAAACTAATTAAACCACTCAAAGTTTCCGAATAATTCTGCTGTTGGTAAAACTGGGTCAACGTTTCCAAGCTTTCGTTAATTTGGGGGCTTTCTTGGCGGTACTTATTGGCAAAGATAAAGGTATGTTTACACATTGATCTCAGGTTCATTAAACCTACTACATCAACAATCACATGGGTTAGTTGGTTTTGGAAGAACCTCACCTTTTCGTTTAAATAGCTAAAAGCACCCTCATAGTCATTTTTCGCTTGGGTAAAAATATCATCAAACTGGTGCTTAGCTTGACTAATAATCTTTAAAGTCTTTTCGGAAATAATTAACTCTTCATTCGCAATTAAGTTTTCAATTTGGAAAAACTTAAAACGAAAGTCATCTAAGGTATAGACAAAAAAGCGGTAATCATCAAACTTATTAGCTGACAGCGCAAACAGCTCGTTTAACAGATTTTTCAATCCATCCAATAACTTCATCGCTTCACTCATTAAGCGAAACAACTTCTCATAATCAATGAATTCGGTCAGCGCATTTTCCTGGTTGAGTAGTTGGGTTTTTTGTGCAATTAACAGCGAGTGTTCGTTAATCTGGTTAATGATGTTAACGGTATCCAAGTTGTTTTGAAACATTAACTCAATTTCCTTCATTAAAGTCTGTAACAAGGCGTTTTGTTGTTGGTAGTGGTTAATTGACTTTAAGAAGCTATCCACATTAGATACCACTAAGCTAATCGCTTTTTCTTTAACACTAACAGCATTAGATAACACATCAATCTGTTCCTTAGCTTGGTTAATGCGCTGTTCACACAACGCTAAGTTAATGTTGCCCAACGCTTGGCTACATTCCGCCAAAGTAGCACCAATATTAGTAATTAATTCCTGAAACTTAGCTACCTCTTGGGAGTTAATTGCTTTGTTTTTCATCGAGCGCAATTGCGTTACCTTTTGACTCAAAATTTGGAAGTGGTAGTTCAAATAGCCTACTTTGACATCAAAGAAGTATTGTCTTGATGCGGTAATAATGGTTTGTGCTAAGTTATTGTGGAGTTTATTCAACGCTTGGTTTTGCTGTTTCACATCCTTATTTTTGATGTGTAGTTCCACATCCTTAAACAATAAGGAAATGCGGTTAATAATGTTGAGGTAAAACAAGGAATCGTACTTATGCACCAAAAAGTCATTAATAAAACTAACTAGTAAACAAAAGGCTTGTCGGTAGAATACTAGGGTATCATAACTACTGTCAAAGTACGCAATTGCGTTTTTAGTCAGCTTAACATAACGCTGTTGCTTGTCATGTAATCCTTGTAAGTTTTTGCGCAACAACTTAATATTACGGTTAAACCAAAAGCAGTTGTAAAGCGCTTTTGGTTCACTAAAAACTAACAGCTGTTCTAAAGCGGAATTGATTTCGCTGTTAAAGCTCTCTTGAAAACTTTTGAGCAATTTCACTGGTTGTTCCAGCGCTTTCTTTTCACTGCGCAACAGTTGAAACGGCACAATTACCGAGTTGAAATTACTGAGTTTTAACTTGTTGTACAGCGCCTTGGTCTGTTCCAAGTATTGCTGGTTTTTATGCACAATAATGAAAATCGTTAAATACCACAAGTAAAACAGAAAAGCTAAGACAGCTAACGATCCCAATAAAGCAGCAATGACAACCATTAGCGTTGCTTATTGGAAAGCTTTAACAGTTCAAACATTAAGTTTTGTTCAATCTTAATGTTGAGCTTTAAGTCCTTCGAGTGGTTCATTTGCCGCTCTAACTCGGCCTTACGCTTCAAAGTCGCTTCCAGATCTATTTCATCCTCAAAGACAAAGTTCTCGGTAAAGATCTTGGCCTCAGTTTTCTTAATGAGCACTAACCCCGCTCCTATGATGGCATTGCGCCGCGTGTTATTGGCAAACAGAATTTTGCAAAAGTGCGATTGTACGGCCGCAATTAAGGGCGCACGGTTATAGTTCAAACCCAGATAACCATTAGTGGTTTTGACTTGCGCACTAATAATGGCTTGATCGAGTTTAATACCACTGGGACTTAAGACTAAAAAGCGCAATGGTTGCATAGTTATTTCTCCTCAACAGTAGCTTTTTTAAGCCGCTTCGCTTTCTCCACTGCTTCCTGAATGGTACCAACAAAGAAGAAGGCCTGTTCGGGGAGATCATCATGCTTACCGTCCAAGATTTCCTTAAACGATTGGATCGTGTCACTTAAAGGGACATACTTCCCAGCAATCCCCGAGAACTTTTCCGCTACAAAGAACGGTTGGGAGAGGAAGTTGCGGATCCGGCGTGCCCGTTCCACAATGATCTTGTCCTCATCCGCTAACTCATCAATTCCCAAGATGGCAATAATGTCTTGTAGTTCCGCAAAGCGCTGTAAGATGTTTTGCACCCCTAACGCTACCTTATAGTGGTTAATCCCGACAATATTAGGATCCAATAAGCGACTGGTAGACTCTAAGGGATTGATCGCTGGAAAAATTCCCAACGCTGCTATGTTCCGGTCTAACACCGTTTTAGCATCCAGGTGGGTAAAGGTGGTAGCCGGAGCGGGGTCAGTCAAGTCATCAGCTGGCACATAGATCGCTTGCACCGAGGTAATCGAACCGGTTTTAGTGGAAGCAATCCGCTCTTGTAATTGCCCCATTTCAGTCGCCAACGTTGGTTGGTAACCCACGGCTGATGGCATCCGACCTAACAGTGCTGATACCTCACTACCAGCTTGGGTAAAACGGAAGATGTTGTCAATGAAGAGCAAGACATCCTGGTTGTCATGGTCACGGAAGTATTCGGCCATAGTAAGCGCCGTTAAAGCGACCCGCATCCGTGCTCCTGGGGGTTCGTTCATCTGACCAAACACTAAAGCAGTTTTGTCAATCACCCCACCCTGGATCATTTCATAGTAGAGATCATTACCTTCCCGGGTGCGTTCCCCCACACCAGCAAACACACTTAAACCAGAGTGTTCCTTAGCAATGTTGTGGATTAACTCTTGCACTAAAACCGTTTTACCAACACCAGCACCACCAAATAAACCAATCTTACCACCACGGGCATAGGGAGTGAGCAAGTCAATCACCTTAATCCCTGTTTCAAAGATTTCTAGCGTATTGGACTGGTCATCAAACGAAGGTGGTTTGCGGTGAATCGGGAGTTGTGGTTTTTTGGGTAACTCCTCCTTCCCGTCAATGGGTTTACCCAAAACATTAAACATCCGGCCCAAGACGTTTTTGCCAACTGGGACTTCAATCGGGTGGTTATAGTTAGTCACCTTTTCGTTGCGTGCTAACCCTTCAGTTGGACCCAAAGCAATGCAGCGCACAATGTCATCGCCTATTAGCTGCGCGGCTTCCAAAAACAGTTCTTCCCCAGCTAACTTAACACTCAAACAATCGTAAATGCGGGGTAACTGACTACTTTCCGTAAAGATGACATCAACTACCGGGCCAATCACTTGGTGGACCTTACCGTATGTAATGTTTTCCTTTTTCATTAACTAATACCTCCTATGATTTCAATAATTTCTTGGGTAATAGAGTTTTGTCTTAACTTATTAAATTGAATACTGTACTTTTCATACAGATCCTTGGCATTCTTAGCAGCGGCATCCATGGCGTTTTGTCTTGATGCTGACTCACACAACTTTGTCTCTACCAACCCACCGTATAACGCAATGTCAAAGAACTGGGGACTAATGAGCTTAATAATTGTCTCCTCATCGGGTTCAAAGTCGAGCTTTTGTTCCATTTTGACCGGGTTGAAGGCCTTAAACATCGCTACATCAAAGGGAAAAACCTGGAAGCTGTTGGCGTGTTGAATTAAGGGGTTTTTAAACTGGGTGTAAATGATGCAAATACGGTCTAATTGGTGTAAATCAAACTGCTCCATAATTTGGTCGGACACCTGTTGACAGTAATCAAAGCTCAAGTCACGATCTTGGATGTCAACATAACCAACGGTGGGGTTATCGGTGTCACCTTTATTTCAAAAAGACTGGTTTTTGCGCCCCAAAAAGAAGATTTTGTCACCGGGTTGGAACTTCGTTTGCAACAACTTGTTCATGTTAGTGTTGTGCTGTCCACAAAGACCCAACGATGAACTCATCATCACCCACAGGGTTTTGGGATTTTCCAGGGGCTTGGGCATCTTCTTTAAGCTGAGAACTACTTGCCCAACGGCCTTATAAAACTCATTGAAGAAGTAGTTAACTTCCTTAAACTGTTTTTTAAAGCGGACAAACTTAGCACGGGACACCATTTTCATTGCGTTAGTAATCTTAATGGTGGACTGCACCGTGGTCATTTTGCGCTTAATTTCCTGGATAAAGGCCATTATTCCAACTCCTCCATCGTTCCGTATTTAGTGATGTCGTAATCTGGTAAAGCACTAACAAAGCGTTTTACTAGTGCCTTAAAAGCAGTGTTAGTTTGTGCTTCCAACGCTTCACTAAAGCTCTTTTGGGTCGCTATTTCCTTGTAAATCGGGTGTTCCTTGTCAAATTCCTCCATAATGCGTTGCTTAAATTTAGCAATGTAATCCAGTGGAATAAACTTAATGAAGGACTTGGCAATCGCAAACAAGAATAACGCTTCATGGGTTTGTGAGTAAGGTTTACCGTTGGGTTGTTTAATCATCTCCATTACCCGCTTACCGCGTTCCAAGATTTGCTTGGTATTTTCGTCCAAGTCACTACCAAACTGACTAAAGCTGTCTAATTCACTGTACTGCGCTAGCTCTAACTTTAAGCTCCCGGTTTGTTGTTTAATTGCTTTAATTTGCGCTGCACTACCAACCCGCGACACCGATAACCCCACGTGGATGGCAGGGCGTTGACCGGAGTTAAACAGGTTTGACACCATGAACAGTTGACCATCGGTAATCGAAATGACGTTGGTCGGAATGTAAGCGGAAATGTCCCCAGCTTGCGTTTCAATAATAGGTAAAGCGGTAATCGAGCCACCACCATGCTCCTCGTTTAAACGGCAAGCACGTTCCAGTAAACGCGAGTGCAAGTAGAACACATCCCCAGGAAAGGCTTCCCGTCCTGGTGGTCGGCGTAACAGTAAGGATAAGGTACGGTAAGCAATCGCGTGCTTGGACAAATCATCAAACACAATTAGAACATCCTTACCCTGTTTGAGCCAGTACTCAGCAATGGTAATGCCAGTAAAGGGCGTGAGGTAGATCATCGAAGCGGGATCACTGGCTGTAGCACACACCACCGTGGTGTACTTCATCGAATCAGTTACCTCTAACTGGTGGACAATTTGCGCTACCGAGGAATTCTTTTGTCCCATCGCTACATAGACACAGTACACGTCCTTACCACGTTGGTTAATAATGGTGTCAATTGCAATTGAGGTCTTACCAGTTTGGCGGTCCCCCACAATTAACTCCCGTTGTCCCTTACCAATCGGAAACAACGCATCAATCGTCAAGATTCCAGTTTCCAGTGGTTGGTTAACGGTTTTGCGGGTCATTACCCCAGGGGCAATCTTCTCCACTTGGTCAAACTCTTCAGCCTTAATCGGACCACGACCATCCACCGGTTCTCCCAGGGCATTGACAATCCGGCCCAACATTACATCACCCACGGGAGTTTGCATCACGTTGTGGGTCCGTTTAGCCGTACTACCTTCACGGATTTTAGAGTAATCACCAAACAACGCCACCCCAACCGTGTTTTGCTCTAGGTTAAGCGCAATCCCCTGCACGTTGTTTTCAAACTCAATTAACTCGTTTAAGAGTGCGTTTTCCAAACCACTGACCTTAGCAATGCCATCAGCGACACTAATAACCTTGCCAATTTCACTGTTAAAGATCTGTTTGGAATACTTCTTAATCTCATTTTTAATGAGTGCTACATATTCATTTAATTTGTCTGCCATAATTAATTCCCTTTAAACTGTTCCATTAAACGTGATAGTTGATCACGGATATTCTTTTCAAATAACTGGGCGCTCGATTCAATTCGGATACCCGAAATCAAGCTCTTGTCAATTTCGGTTTTGTAAACAATCTTGGAATTGAGCTTCTTTTCCATTGCCGCTACGATTCGCTTGAGTTGGGGCGCACTCAGTTCAAAAGCGGTAATGATCCTAATGAACTGGACGTGTTTTTCATTCTCAACACAATCAAAGAAGTACTTTAAGGCTTGCTCGACTAGGTTAAAGTAGCCCTTGAGCACAATCGTTTTTAAAAAGTCAACAAAGAGGTCACAAAAGTGGTTTTTAAACAGCTTATCTATTAGCTTAAGCTTTTCCTTTTTGGTTAAAGTATAAGCATTGAGTAGAGCAGCTAGTGATCCATCTTTAAAGTTACGCGCTAGTTTTAAAAAATCATGGCTCTGGTCATAGACCTTCTTAACCTTCTTTTCCTCTTCACTAAGTTGGAAGAGTGCCATTCCAAAAGCCTGCGCGTTAATCATGGTCGTCCTCTGCTTCTAGTTCGCGGATAAACTCTTCAATAAAGGCCTTGTCGGCTTTGACATCCACCTTTTTGATAATCAATTCCTTAGCAGCTAACATGGCTAGCTCGACACTTTCCTTTAAGGATTGTTCTTGGATCAAGCGCTTTTCCTTTTCAATTTCCTGACGCGCTTGGAAGATCATTAAGTTAGCTTGCCGGTTAGCCTTCTTTTCGATTTCACTTTTAAGTTGCAACGCCTCATAGTTGGCCTGGTCTACAATTTCCTTAGCAACTACCAGTGAGTTTTCATGGCGTTGGTTGGCTTGTTGGAGTAATGCTTGAGCTTGTTGTTCTAAGCTGTTGGCCTGGTTGACTTGCTCTTCCAAAAGCGCTTTGCGTTGGTTTAAGAACTTTTGTGTGGGTTTTCAAAAGAGGAACAACAACAAGAACAACAGGATCACAAAGGCCAACAAGTGGGCTAAAAAGACCCAGAGGTTCGGAAAAAGTTCGTTGATGACTGATTCACTTTTAATTTCCTTAACGTTTTCGGTACAGGAAACTAAAAACAAGGCAAACAGGGCAAAACTAAGCGCAACTAAGGTCGTTTTAAATACAAAAGTTGCTCGTAGTTTCATGATTAAGCGCCCGATACAAAGATAAGAATGAAGGCTATTAACAAGGAATAGATGGAACTCGATTCACTAATAGCAGAGCCAATAAAGATTAATTTAAAGACCTGCTTTTCCACTTCGGGGTTCCGCGCTACGGCTTCAACCGCTTTCCCAAAGATGTAACCTTGACCAAGACCCACGGTCGCACCACCGACCATGGTAATCCCAGCCCCAATGTAAGCCCCTAAACGTTCGGTACTTTTATTGGCAACATTGGTATTAGCTGTTGTTGTTTCGTGTAAGATGCGGCCAACTGTGGCGAGAATTTCATTAACGTGTTCCATATCTAATTAATTATTTCTTTTGCTACCTCCAAATTTTGCAAACGCTGCGCTGCTTCTTGACTTTCTTCCAAGCCAATGTTACGCATTTTTGCCCAATAATTATAAGTCAACATCACAAAGACATATCCCTGCAGTGTACCCACTACTACATCAAAATATACATGTAGGGCTGGGGTGAGAATTCCTGCAAAAATGGCACCTAAACTAAGCGCTAGTGGCTTATTGCTTAAAGTCGAAAAAGCAAAGAACCAGAAGTTATAAAAGAGCGCTAAAATCAAGGTTCCCCCAAGGATATTGCCCCACAAACGAAGCGAAATCGAGAACAGTGGCGCAAACTCACCCAAGAAGCTCAACGGATTGGGAATTAAAGTGGAGTACTTCTTGCCCTTAATCGTAATGTTGAAGGTAAAGCTTTTAAAGAAGGATCACCGTTGGTACCTAATCCCCATAATGACAATTCCCAAAAAGGTGGCTAGCCCTAAAGAAAAGGTAAAGGTCAGTGATGAAGTTGGTGGGGAAATGCCACCTAATAATCCAATCAAGTTACTGGAAACCAGGTACAACAGCAACATTAAAAAGTAAGGGGTAAACTTCTTGTTTTCCTCACCAATTAAATCCGCTGTAGTATCTTGGGCTCAAACAAAGAGCATTTGCATTAATAACACAAAGCTCGAATGGTTGCGTAACGGATCCGCTTTTCTTAACTTGATTCAGTAAACCAAGAATAAGGTGAGCAACACAATAAAGACCACAAAGATGCCAAAAACTTGATCAGTGGGTAGCAATGGTTTTCATCCCGCAACTGGGGAAATGTCAAAGATACCTTCGTGTGGTACGAGGTTAACTCCATTAGGTTCTTTTAAAACTATCGCCCGTGGTGACATGCTTCTTATTGAGCTTGGAAATCAGGTTTTTGCAATGATATTCCAAAGGTAGCCATGCAAATGATATCACTATTTTTAGCAATGGGAAAAATAACGTTGAAATTATTGTGGCAATCACATGAAAATATTGCGGTCTTATAAAAAAGGCGAGCAATACGGGAATCAGGTACAGCAAATACCGTGCCACAAAAAAGGAACCAAAGAAAACCGTAACTCGTTGTTTTGATAAATGCTTAAACTTTTCAAAAAAGTCCCCTAGTTTTAATAAACCAACCACGCTTAACGCTACAAACGGAAAGGGTAGTAACCAACCGTATAAAACTTTTAAGTGTTGGGTAGTTAAAGCTCAAACTAAAATACCCACTAAAACCACTAACCAAAGCACCAAGATAATGAGGGCAATTGCTTTAGTGAGTTTTTTATTGGTCCAGTTGTACAAGGCCGTTAAGCTTTTTGCGGTTTAATGTTTTTAAAGGTGTTTCAACCTAAGTACTTAGCATTATTGCCCAATTCCAGTTCAATTTGCAATAAGCGATTGTATTTGGCAATTCGTTCGGAGCGGGACATCGAACCAGTTTTAATTTGACCAGTGCAAGCCGCTACTGCTAGATCAGCAATTGTCGTGTCTTCGGTTTCCCCACTACGGTGGGAAATTACCTGACTCCAGTTAGCGTCCTTGGCAATGTTAATCGCTTTAATTGTTTCTGTAAGGGTACCAATTTGGTTGAGTTTAATTAAGATCGAGTTGGTAGCTTTGTGAGCAACACCTTTTTCAGCAATTGCTGGATTGGTGCAGTACAGATCATCACCAACTATTTGAATGTGTTGACCCAGTGTTTTAGTTAATGTTTCCATACCTTCCCAGTCGTGTTCGCTAAGACCATCTTCTATGGAAATAATAGGGTACTGCTCAGTAAGCTTCTTGAGGTAAGCGATCATTTGGGCAGTAGTTAAACTCCATTCCTTCTCGTCAAGAATTTTGGCCTTAATTCCTTTTTTGAAGACATAGCGCTTGGTGGTGTCATCATAAAACTCACTCGCTGCTACATCCAGTGCAATCGCAATGTCACTACCTGGTTGGTACCCAGCTGCCTTAATTGCTTCGACCATTAAATCAAGCGCTTCTTCCGCTAACTTTAAGTTAGGCGCAAAACCACCTTCATCACCCTTATTGGTGTTTAAACCCCGTTGCTTTAACAGCTTTTGTAAAGCATGGAAGGTTTCACTCGCCATCTTCAATGCTTGGTGCATCGAGTTAGCACCCAATGGCATAATCATGAACTCCTGGAAGTCAATGTTATTGTCAGCATGTGCTCCACCGTTAATGACGTTTAGCATTGGTACGGTGAGGATAAACTCGGTTTTATTTAGACCCATGACCTGGTTAGCAAGGTACTTAAACAAGGAAGTCTTTTGGGCGCTAGCAGCTGCTTTAGCCACAGCTAGAGAAACTGCCAAAATGGCATTAGCACCTAACTTAGCCTTATTGGGGGTACCATCCAGTTGGATCATTAAAGCATCAATGGCTGCTTGATCAGTCGCATTTAAACCAATTAGTTTAGGGGCAATAGTTTGGTTAACGTTTTGCACAGCTTGGCTCACCCCTTTGCCAAAGTAAGCTTTGGGATCACCATCGCGTAGTTCAATCGCTTCTTTTTCCCCAGTAGAAGCCCCAGATGGAACCATCGCTTCTCCAGTATGACCACTAGCTAACTTCACAACACAAGCAACAGTGGGAAAACCCCGTGAATCAAAAACTTGGTAAGCAAAAAGATCAGCAATCTTGAAAAGATCGGTTCCAGTTTGTGCACTCATATGACGCTTCTAACTAATATTTACTTAAATTATATTGACCAATAAATTTAGGAAATGAAACAAATCTATTTTGGTGGTGGCTGTTTTTGGGGTACCCAAAAGTATTTTGATCTGATTAAGGGTGTCCAAAAGACCAGTGTGGGCTACCTCAATTCCAACATGAAAAATCCCACTTACGAACAAGTATGTAGTGGGCAGACCAACGCAGTGGAAGCAGTGTTCGTTGAATATGATGAAAACAAGGTAAGCTTAAACGAACTTATTGATGCTTTCTTTAAGGTCATTGATCCCACCATTAGAAACCGTCAGGGTAATGATATTGGTACCCAGTACCGTACCGGAGTTTATTGGGTAGATCCCCAAGACGAGCAGTTAATTACGCAAAAGTTTAGGGAACTACAGGCCAATTATCCAAAACCAATTGTGACGGAAAACCGTGCAATGGAAAACTATTTTTTAGCAGAAGAGTACCACCAGGATTACTTAAAGAAAAATCCTCATGGTTACTGTCACATTAAATTTGACTAGTTAAAGACTTCTGGGTTTTTCACAAACAGGAATGCCTTAATGATGTTGTAGCAATAGTCATTAATCCGTTCAATGTGTTTCACCGACAGAATTAAGTCAATTAAGAAGATGCGATCACTCAGCTTCTCGTCTTTAACCTTGTCATAGTATTCAGTTAACACGACGCGGTACTGCTGTTCAAACTCTGTCAGAATTTTAGTGACATTCTCAAACTGAATAGTAAGCGGTTTTTCCTTATCTTGTAAACTGGCAAACGTTTGCTTTAAGTTGTTCAACACACTTTTGTGCAAATTAACTAACTTACTAAAGATGCTGACATCCAAGTGCTGGTAGTGCTTGACAAACTTAGTGAGGTTATTGGCATAATCACAAATGCGTTCCAAATCACGACTACACATAATGATCGTAATTGTTAAGCGTAAGTGGTTAGTTAACGGGGAGTTTTTTGAAATCGCTCAAATGGATTCGTTAATTAGTTTAAACTCCGATTTATTGGAAAAGTCTTCCATTTCGTAAATTGCTTGAATCAACTTTTCGCGCTTAGTTACATCCGCTTCACATAACAGTTTATTGAGTGTTTCATGCATCTTAATGACGTGCTGAAAGTAATCAAAAAAGAGTCCTAACAGCTTTTTTTCTGAACGCTTTAAAATTTGGTAGTTAATGCTTTCCATCGTTAATTCCTCCCACTAATGTAACGGTTAGTTTCCTTTTGCTTTGGTCTCGTAAAGATTTGCTTGGTAGTACCTTGTTCCACTACCCTTCCGTTAGCAAAGAAAATCGTTTCATCAGTGATTCTAATAGTTTGTGCCATTGAGTGAGTCACAATAATAATCGTGTATTTTTCCTTTAATTGCTGGATTAACAGCTCAATCGAGTTAGTGGCAATTGAGTCCAACGCACTAGTTGGTTCATCCATTAATAAGACATCGGGTTGTAGAGCAATGGCTCTGGCAATACACAACCGTTGTTGTTGACCACCCGAAAGGGTATTGGCATTACGGTGTAAGTTATCTTTGACCTCGTCCCATAAGGCGGTTGACTTTAAGGCCTGTTCCACAATTTCATGAATCGCTTGCTTGTTATGAATGCCATGGGCTCTCAGTCCATAGGCAATGTTTTCAAAGATGGAAAAGTTAAAAGGGGTGAGTTGCTGGAACACCATACCAACCCGTGTGCGCAAGGTTAAATCATTAATAATTCCGGAATTAATGTTTTTACCCAAAAAGAAAATCTTGCCATTTCAGCGCACATTTTCATTGAGGTCATTTAATTTATTTAAACAACGGATAAAGGTGGACTTACCACAACCGGATTTACCAATTAAAGCGGTAATCTTATTGCGCTTAATTTTGAGGTTAATGTCAAATAAGACCTGCTTGTCCTTGTTGTACCAGAAGTTAAGGTTTTCAATTTCAAAGACATTCTTCTTGTCAAAGTCTTGTTTGACAACATCGTCCTTGGTATTCTTAATTTTGATGAGGCGTTGCAACACATCAATTTCACTCTGCAAGTTAGCAATTTCCTCAGCTTTGGTAGTGGGATCTAACTTAGCAACTTTTTGCTGGTATTGCTTAATTTGTTTTTCATATAAAGCACGGTACTGCTTCACCTTTTCGCGCTTTTGGATGAAGTTGTGTCAAATTGTTTTTAGGCCTTTTTTCATAATTGCAATTTGCTTTTAATTTGTAATCACTTTTGTTTATTAAACGAAAACAGGTAAGGAATTAAGTAAGAGCTTAAAAAGATTAAGAACATCAGGAAGACAATTGATACCAAAGCGGTTTCCAGCATTACATCAACCGCCGTACTGTTAGTGGAAAATAACTGACCATAAATTCTTGTGGTTAAGGTTTGACCCGGTAGACTTAAATCAAACAAGTTGCTGGATGCTAACCCAGCGGTAATAAAGAAAGGAGCTGTTTCCGCAATAATTCTGTTAATCGTTAAAATAAGCGCAATAATTAAACCCTTCAACGCCCCTGGTAACACAATTTTCCAAATTACTTCCCTTTTACTTACACCTAAGGCATAAGCACTTACCCGCAAGTCCCAACTGACATTATTGAGTGCTTCCTGACATGTGCGAATTAAAAAGGGTAAGACCACTACACTAATAGTAAGAATACCCGCCATTAAACTAGTACCATTCGCACCACCCGCACTTAATTGCAGTGTTCGCAAAAAGAAGGATAGACCAAACAATCCGTAAATAATCGAGGGCATCGAGCTGAGCGAATCAATTACAAAACTAAAGGTATTTTTTGCAATCCGCGACTTCGTGTATTCGTTTAATCAAATGGCAATTAACAAAGCGATTGGAAAGGTAATACCAATGGCAACCAGAATAATCACCAAGGTATTGACTAAAGCTCGCCCTGTAGTATCTACACCAAACGAAAAGACAGTGGAACCACTGCGCTGAATCGCTTGACTACCATTAATAAAAACAAACAAGAGAATCCCTAACACAAAACCAAACGCTAAGACGGCACAAAAAATTTCCTGCAGGATTTTTCACGATGTGTAAACCTTATTTAAGTGGTTAGTCTGCATCCTTTCCTTGAAGAAGGTAGCGCGCTGCTCAACATTAGCAGCTGTAATCTTTTTAACCTGTTGTCTTCTAGCAGTTAAATCAGTAAAAAGAGCGCCAATGTTATTAGGAATAAACCACACTACTTGATAAATAAAGTTACTAATCTTCTTTAGGAAAGGATAGCGTTCCAGCGTTTTTGGCCGGGTCGATCAAATGGCAAAAAAGTTCAAGAAGGAAATTAAGACAAAGATAATAATGCCAAAAGTGTATAAGACCCCACTAACCTGTTCATCGCCGTTTTCCGAAAAGATAAAGGTGGAGATAACAGAACCCAAAGTTTTGAGATCTGAAGTAAAGAAACGATCATTAGCAATCACTTCCTGGTAGTTAACACTCTGCAAAATAAAGTTCACAGCCATCGTTTCGCTAATGGCACGACCAAACGCTAATACCAAGATTACTACTAGTTGGGCTTTGATTTCCTTTTTAATTACCTTATAAATCACACTGGTTTTGTTTTCACCAAGTGAAATGGCAACCGTCATTAAGCTAGATTCCACGTGCAGTAAGGCATTGGTGGTTAAGGAAATCACAATCGGAATGATCATGAAGGACAGCATCACAATCACGTTAAGCAGTGATAAGGGTGGCAAGTGCAACACGTCACGGAAGATACTGCTGAGAATTTGGGTAGCAAACAATCCAAAGATCACTGAAGGGATCCCGGACAGAATGTCAATTACTAAAAGTAGTTTTTTGCGAATCCGGGGTTTGCAACGATAAACCAAGAAAATCGAAGTGCGAACACCGATATAGCTCGCAATAATTAACGAGCCAATCGAAACCACAAAACTAACTAACAGCGGAAACCAGATGCTCGCTTTTTTAGCACCCAAGTTAAATTCCAAACCCAGTAAGGACTTGGTAAAGTCAGGACCAGTTTTAGCTGCTTCAATTAAGAGGAAAATAAAGAAGCTAATGAAGAGCAGTAAAAAACAAACAGCCACTGTTTGCGATAAGTACCGCAGCCAGTTGTCTTTTTTTAAACGGCTTTTAATTTTCTGTTTCACTAGAGTGCACCGAAGGTTTGGTTGTTCAGACTGTAATCATCAACCCAGAAGTTGGCAATGTTGCTCATTTCTGCATTACCTGTTTTAAACATCTTTTTCTTTTCGTCAGCCGTTAACTCTAACACACCAAAGTCATCGTATATTTTCTTAATTTCACTGTTGTTGCTAAATAACAATCAGTTAAAAAAGCGTTGGATACTTGGCAGTGTTTTTTGTTTTGGTAACAGGGAAACCACTGAGTTCAACGGGCGTACCCATTTGTAAGTGTTGCTAGAAACTGCTTGACTGGAGGGGGTAACTGCGTTATTATCATATTTGACTTTTAAAACCTCAAAACCTTCACTCTTAATTAGGTTCATGTTGTTATTCACAAACCCTAAACTGAGGTACACCATGCCGTATAAATTGGGTTGTCGCAAAGTTGTGACAAAGGTATTAAAGGCCTCAATGTTGGTTTCACTAGTTGGTCGCGCATTGGGGCCATAGTTGCGTTGACCTTCCAAAATTTCTTTGGAATCTTTATCTAAAGTTTTTGTCTGTGTAAGACCCGAAAACTTCAAGAAAGCTTCAGCGGTACCAGAAGCAAAAGCGCCCCCAGTACGGGGGAAGCCTATCAAATTATGATTACTGTTTTTAGTGGTTTGTCCGTTTTCGACAAACTTATTAATGTCAACGCTTTTGCTACCAGCGAACAGATCGTAAAGATCGTTGAGGTTGTCTTTAGTGAGAACTAGTTTGCCCTTAAACTCTGATGGTGCTTTATAAATAACAGCAATGGCATCTTTGCCAAGTGTAATTGTTTTGAGCTTTTTGTCCATCCACAACTGCTTGTTTTCCTCAGCATAGCTCTTGGTGTTTTTCGAGACATTACCAATGTCAGCTAGTCCCTTGGTGATTGCTTTCACCCCAGCACTAGAGCCACCCGCTTGGACACTAATTTCTACAAGGTTATCCTTGTCATTGTGGTTCAAGACATAGTGCGAACTGAGTTTGCTTAACAACGGTTGTACCGAAGAAGAACCAACAGCACTAATTAAATTAATATTGGCACAACCGCTAAAAATAACAGCGGTTAGTGATAGTAATGCTAGTCTAGAAAGTCTTGCAACTCTTAGCACTTTTCTAATTGTGTGCGTTAATTTTAGAAAAACTGGCATTTTCGGTACTTTTTAACCAACTTTTAATTAAGCAAGTTGCATTTCTTAGTTGTGCTTTAAGTAGTTAAAAACAACGATTTTCTGGCGTTTATTCTTTGCCAATTGTTTCCTTTGCTAATTTCTTTTTACGAGAGGCTGCTTTTCTTTTAATCATTCAACCAATGGCCGTGGCACCTAAAGCTAACACTCCAGCAATCGCTGATAAAGATCAGGTCAACATCTGGTTAGTTTTAGCACTAGTTTAATCAGTGTCATCTTTATTGTCCAACTTTTCCAGTGCAAAGGCTTTGTTTTGGGTGTGGAAATCAAAGACTGGTTTTTGTCTAAATTGGAAGTTGTTTTCCTTTAAGAATCTAGCTAGTTGTTGTGCTAGCTTAGTTTGACTGTCATTTTTAATGTTGAACCAAACTGACTTTTCTAGACCATCTTTATTAATGAGATATAGCATTTGCTTATTTTGTGATAGCTTTAAGTCATAGTTAATTGCAAAAAGTCTTTGACTACCCAACATTGCTTGAATGGAGAGCTGACTAGTATAGCGATCAACATGGAACTCTAAGTTGAACTTTTGGTTGTTTTTTAAAACGGTTAAATAAGCCATTTTATTTACCGGATTCAACCTAGCATTCTTCGTTTCCACATGGAGGTTCATTTGACTCAAAACAATGTTGAGTTTTTCCAATAACACTTGTTCATCTTGCAAACTAAAGTCTGATTGAAACTCAAACTCATAGTCATCTATTAATTGGATGTTGGATGTTTCTATATCTAATTTGCCAATTTCGAATTCATCAAACATAAAGCTCACTGTTTGGGTTTCCAAGTGGGGTTTAAACCACAACTTTTGTTTGTCAAGCAACAGCTTGCGCGTATTAATTTTGAACAAGTAAGTGCGTGTGTTGGGCTTTATTTGATTTTGTTTAACAAAGTATATTTGCCCTTTTATAATTTCGGGATCGATCGTGGAACCTAACTTAAACAGTTCGTTGCTGTTGTGGATGTCGGCTAACACATAGAAGAAACCTTTGTTTTCGTTCATGTTTCCTAAATCAAGTTTGTATTGTCGGGGATCTTCCACAAAGGTTAAGCTGTTAGCATCTACATCTAACACGGTTTTATCAACACCAGCAAAACTGTTTTTAATGACAAAAACAATTTGTTGCTTTTCAAAATCATTGAGTTTTTCCATGCGTTGCTTTAACTCACCTACACTAAAGCTTTTAATCGCTTTGTGTAACGGAACACTAAAGTCCTTTTCTTTAAATTGCTGGTTGCTAAAGTTACTGCGATATTTAAATTTGTACTGGTAACCTTCTGGAATTTTGTTGATCGTTGGTAGGTTTTTCACTGCACCAAAAAGTTGATCAACTACTTGGAAGTGCAAGTTTTCTGATTCATAACCGTCAACAATAAAATCCAAACTTAAAAAGTTAATGGTGTATTCGTTGGAAGCGTGTTGTTGTGGTGTCAAGTATTTTTCTCAAGTTTTGATATCAAACACATTGACATTGTTTTTAAGCAACGTAATTTCTTGCAAGGTATCACCAATTTTGACTTTAGCTAAAAGTTTGGCTCGTGCAAAAATAGTTTGCAACATCTCTGGTAAGTTCCTAATGAAGATAAGGTGTTCTTTATTGGTAGGCTCCATCCCTAATAAGTCATAGAGGTACTGCAAGCTAGCAAAATTAACGTTGCTGTCCTTGGACTTTAAAGAGTCAAACTTTTGTAGCGCTGTTGCTTTATCAGTAGCTAAATCAATCTCTTCGTTCAACTTACTAATTTGGGCAATCGCTTGAGTAAACTCCTTTTTGAACATTATTTGTAATTGATCTTTTTGAGATAGATCTTTTTCATTAAATGTTTTGGACAGAGTATCCACTACCGAATTAGGGTCAAATTGTAATTGTGAAAGTCACAACCTAGAAAGTTGACTAAAACTAATAGGTGGTTTTAGATCTGCATTATGTTGTTCCACATAAAAGTCAACACCAAAGCGCTGAGCGTTTACTGGGATAATTGGGGTGTATTTGAAAATGTCCCACTTATTGCTAAACAAACTTTGTTCCTCATCATCAGTTAATGGCTTTACTACAACGTTTTTACCCATATTCAAGATTGATTGGGTTACTTGGCGGTTTCTAGTAAAGGCTTCCATGATCTTACGATCTTTAATTACCTTATCTTTAAAACCATTGGAACTACTTCAATATGCATTTGCTTGCTTGTTATCAATGGAAAATACTCAAGTTTCTTTTGGTAATACTGGCTCATTCCACTGAACATTATCTTGTTCAAATACTCTAGGTGTTTTAATTAACTCCACCTCTCAAAAAACACGATAAATGTAAATGGGAGTTGATTCCAACCACCAGTTATTTCAAAGAGTGGCAATGAAAATATTGTTTGGTCTATCTTCAAAGAAACCATCATTTTGTGTTATTGCATAGGCATTAAAAATAATAAAGTAATTGCCATTTTTTCCTTGTTTAATTCACTTAGCATTTTGAACAATGTCATTTATGTTAGGTGACTTAACTGATAAGGAACGGTTCATAAAACCGAAAAAAACATTAATCCATTTAAAGTCTTTATTTCTATTCCATACAGGACTAAATAGCCTTTGGGTTTCTAAGTTATAAGGAATAGATCATCCTGCGTTTTTAGCTTCTTCGGCAGTTCGACCAGCGCTTAATGTCACATATTTATCATGCTGTGGATACCACGGGTCATTAATGCGAGATGGTCCAAAACCAGACTTACTATCATTGTGAAAGTGGAAATACTGAAGGTCGTGTATATCTGATTTAAAGGAGTTTCTAACTCCGGGATAGCTAAAAGTTTTGTTCCATGCCAAACTGCTAGTATAGCTACTTTCAATGCCTAAGTTAACATCTTTGATGTAATAGATATTGTTATACCAATTAAAGTTATTTTCTCTAAGCAATTGGTTATACCGGTCATAATAGCCATCCGCTAGCTGAGTTCAAAGGGTGGAATAGTTTCAACCAAAAGTAGTTTTGTTATTTGGTTTATAAATTAAAGGATTTTCGAAATTAAAGTCTTTACGGAAATAACTATCCTTTAATTTGATCCCAACAGTAAACCCTTGATTGTGGTGTGCTGAGTTAGAAATATACAAACTACTATCAACAAGATTTTTATCCAACGACGCGTTCGCTAAAAGAGTTGGGAACGCAACAAAACCTAATAAAGGTAAATAATGATATGAGCGCATAATTAATTCACTTTTTCATCTATTAAATTGCTCAAAAAGGGAATTGACGCCAATTTTTTAAAAGTTTTTATTTATCCGTTTTGACAACTGCTTTTTGTTATTTTTTAAAAGTTTTTATTTATCCGTTTTGACAACTGCTTTTTGTTTGTGCGATTTCAAATTCTTTTTGCGCTTGTAAAAGTAAAAACTAAAAGCAACTACCACTAAAAACAGACTACTAGCAGCAATTACTAAGTAACCAATAAAATTGGTGTTTTTATTTTCACCATTATTTGGTTGAGCCTGTTTGGCGATTTTAAAAGTTGCGGTATTATCCTGCAGTTTAAGCGGAATCTTTTCTTTTAAGTACATCTGCTTACTTTGAAGAAATAACTGCAAGGTATCGAGCAATTTAGCTTGGTATCGTTGATCAGCAACAGACCAGATATACATGCCTAAATGCTTGTTGCGGTTAACAAGTTGTAGAGTGTTTGAATTTTTAATCACTAGTTCATAATCCAACTTAAATAATGGTTGTTCGTCAACCTTAACAATTACATTAAGTTCACTAGCTTTTGGATTAAATTCAACATCAAAATCAAACGATTGATCTATTGACTTTGTAGTGAGTCTTATTTGTTTGGGAAAAGTTTCTATGTTAGTAGCTTGTTCATCAACCATTAAGTTCATGCCAACTAATGCAAGGTTCAGTCTTCTTATTAAAAGTTGCTTATCAGCTAACGTTAAGGTAGAAACCAAATCTACTTGGTAATCGTTAAAGAAAGCAACGTTAGGCAATTTGGGATCAAATTGATCTAATTCAATCCCGTCTACAATTACCTTAACTTTGTCATTGTTAGCTTGAAATCAAACGTCATTGGCATTCAAAGCAATTTTGTGTGTTCTCATTTGAAACAAGTAAGTACGAATTGTTGGAATCTTTGTTTCTTTTTTGAGAAAGTACATCTGGCCTTTAATAATTTCCCGATTGGAATCTGTGCCTCATTTAAAGAAATTATTGCTGTTAGTAATTTCTGCTGCAATATAGAAGATGCCCGTTTTAGGATTTGTCGGAATTAAACCAGGAATTAGTTCTTCAGGATTTTTAACTAATTTGATTGAATTTAAGGCAACAGTTAATGCCGTATCATCCTGAAATGAAAAAACCATTTTTAATTACAAAAGCTATTTGGTTTTTCTGCTCTTCAGTCAATTCTTTATCTTGGATGTCTAAAACGCTAAACTCCTTGCCGTTATTAACGACGGGAATGCTAAAACGATATTTATTTAAATACTGTTCGTTGTAATTTGAGCGAAACCTTAAACGGTATTGAATGCCCTGGTTTGTTTTTTGAATGGTTGGTTTATTTTTAGCAGATTCAAATAAAGAAAACACAGGTAAATTCTTCGCTGTTCAACTAGGATATTGATCTGATATGAAATCAAAAGAGAGAAATTCAACTTCAAATAAATTAATGCTAATTTGATCTAAATTTAAGAATTGTTCAAACTGGCCTAGATTAAAAACGTTATTGTTGTTTTTAACTAAAGTAATCTTTCTTAATTGCCCACCCATCCTAACTTGAAAGTTAATGGATGCAGACTCAAAAATTGTTTTTAAAAGCCTTGGTAAATCACGAACAAGACTTAATTCTTGAGGATCCCTAAAATCAAAATTTAGCAGTTTATAAAGGTAGCTTAGGCGTTCTCATTGCTGTTTTCGTTCAACATTTTTAAGCGTTAAGAAATTTTGTAAAACTGTATTTTTATCAATTGCTTGATCAACTAACTGATTAAGCTCTGTAATTTCTTGAATCGCTAAGGTAAATTCCCTTTTAAAACGTAATTTCAAAAGAGCTTTTTGGCCAAGCGTTCGGTTATTAAAGGTGATAGAAGTATTATCAACTATTGAATCAGGGTCAAACTCTAATTGAACTAAAATAATTCGCGATAGCTGATTGAACTGAATAGGTGATTGATTTTCGCTCTCTACTAAATTTTTTGTTAAGTAAAACGAGCTGTCAATTACATGTGAATTGATAGTTGCGCTAGAGAGCAACAGTGGGGTTAATGAAAAACCCATTAATTTTCAATATTTCAAAAAAGGCATGGCAATCTTTACATTCAATAAATTGAACTAAAGTTTTTAACTTGCCAATTTTTAGAAAATTAGTGCTTTAAAGCTTTTTAAATTACTAAGCTAATTGTTTGGAAAAGTGAGCAATCTTGCTAAAGATTAAATGGTAGTAATAATCCAATTGCTTTTTGCGCATTTCGATTTCAGCAGGAATACCTTCTACTAAATCATTGCAAAGTTTTTCAAAGGCAAAGAGAATATCAGCAATCTTTTCTTGAATTTCTAGAGGTGGAAAAGAGAGTTCAATTTCAGCCATTACTTTTTGGCTTAGTTTTGGTCTTGAAGCTAGATTATGAACGAATTTTGGTGCTTCTATCTTTAATAAAAAGGATAGGAATTTGGTGCAGATTTTTTTATTTTTAACTTTTAAAACACCGCAATCTTGTGATGCATTAAACTTGCCATTACGATAAAAAACTACTCCAGCATAACCATTTGTAGTTCAAGTGATGTATTCACCATCAAAGTCGCAATCTTTAATGCGTCCTAACTCACCATCATTTGTGGTAGCTGCGGAATAAACTGGATTTTCTCCAGGATTATTCCTAATATACGCTTTAGTGATCGCTCGACCTCTTCTAATTTCACAAATATCCTTTACTTGAAAAGTTTCGAAAGGTATATTTGCTCCGTAAATTTTTCGGATATTTTCTTGGTTGAGCAGATAATCGCGATAGAAGGCATACTGCTTCTTTCGCTCTCTTAGCTCGGCGCTTAGCTCGGTAAAAGTATCTAAGATAGTAGCAATTTTCTCTTGAATTTGCAAAGGTGGGAAATCTAGCTCAATTTCTGCTAAAATTCCTTGCATTAATTTAGGAATAGGGCAAGCATAATTAACAAATTTAGGTGTTTCTAATCTAAGTGCATAAGCTAAAAACTTAGGATTAATTTCATCACTTTTTACCTTAAGAATTCCACAAACATTGGTTGCGTTAAATTGACCGTTACGATAAAAAACACTACCAGCTTGGGCTCCATCAGTTGTTCAAGTGACGTACTCTCCATTAAAATCACAATCCTTTATCTTACCGATTTCACCGTTATTAACTGTTGCTGATGAATAAACAGGGAATTCTCCTGGATTGTTTTTGATATATAGTTTGCTGATAACTCTACCGCGTTTAATATCGCAAATATCTTTGATTTTGAAACGTTTAATCAACACAGTAAAACTTAATCTCCTTTAAGTTTAAAGATTAAGTGGGGAACTGGACAGCTCAAAGTTTATTGAAAAAAACTAACGCTTGGTAAATTGTGGAGCACGGCGGGCACCGTATAAACCGAACTTCTTCCGTTCTTTAACCCGCTTGTCACGGGTAGTTAGTTTCTTGGACTTGAGAATCTTTTTCAATTCTGGATTGAACTGTAAAAGCGCGCGAACAATTCCTAAACGAATTGCACCAGCTTGTCCGGTAAAACCACCACCTTTAACTACCACATTGATATCAAAGTTCTTTTTAAGATCAGTGAGATCTAAAGGTTGTTCCATGTCTTGGATCACTAGTTTATTAGGAAAGTATTCACTAGGATCACGACGGTTAACAGTAATCTTACCCTTGTCTTGGGTAGGCGTTAGATAAACTTTAGCACTTGAAGACTTACGGCGTCCAAGTCCATAATAAGATTGTTTTTCCATTTATATTAGCTTCAATTTAAAGTGGTAGGTTTTTGCGCTTCCATGTTGTGTTTATCACCTTTAAACACGTGTACCTTGGTGATTCAACGACGGCTTAAACGGTTATCAGGTAACATGCCCTTAACAGCATTATAGACCAGTTTGTCGGATTGTTTTTTCAACATATCACGACCACTTACCTTCTTAATGCCACCAATGTATTGGGAGTGGTGGTACCAAAACTCGTTTTCTTTTTTATTGCCAGTCAACACAACTTGATCACTGTTAATAATGATCAAGTAATCACCACAATCTTGGTTAGGGGTGAAATCAACTTTATTCTTGCCGCGAATAAGATCGGCCGCTTTAACCGCTAACTTACCGAGAACGAGCCCAGCAGCGTCGACAATATACCACTGTCGTCGCTTGTTGGCCTGTTCTTTTGTTAGCATTGATGTTTTTTGCATATTAACAAAACTGCTTATTATTCTAATTTATTACTTTTCTTCATAAAGCCCTTTGAAGAACGTATCAGCGATCTCCTGTGCCGCTGTTTTTTGAGCTTTTTTAACAACGGTACTACTTAACTGTTCTAACTGTTGTAATTGCAACACTTGGGGTTTATGCGTTAGTTGTTCCACTTTAGCTAGTACCTTAGTTTCATATTCACTCAAAGTAAAGGAACTCAAGAACAGGTTTTGTTGAAAAATTTCTAACAAGGTGGCTTGGGCAGTCGCTTCGGTAAGCGCTTCCTTTCACAACCTTGTTGCTATCTCTTTATCTTCGAAAATTAAGACTAAACCAAAGGGTGATGCTGCTAGAACCTTAATAACTTTACTTAAATCACTAGCGGTAAACTTATCGGAAAACTGCTTTAAGTAACTGCTGTCTTTAGTTTTTTCTGCTTGGGTGAACTCCCTTTTAAAGCTGTCCAATAACTGTTGGAAGTGGGCTACTAGTTTAGGATCATTGTGCCCATAAACAGAAACGGCAATCACAGCCCGGTTAAGTTTTATTGGGATGGAAACTGGGTTATTATCTGATGGTTCTATTAACTTAATAGGAGCAGGCTTGCTTACAGGCTGTATTAATTTTTTAACAGCGGGCTGAAAAAAATCAGTGTTTTCTACAGAAAGAGGTCTACGACCGCGTTTGCGTACCACCTCTGGTTTAATCGCTAACCCAAATAAATCAGTTGCTGGAGCTGGTGAAGTTTCTGTTGGTTGTTCTACTTCTGTAATTTCTTGCTGTGCTTCTGGTTGCACTATAGTTTGGGGTTGTTCTATTAGTTTAGAAGCAACAGGATCTTTTACGAATGATGAAACTTTAGGTTCTTCTATTACTTTATAAAGAACAGGTTCTTTTGCTGGTAGTTGGGGCTTGGCCTCTTCTACAGTTTTTGAAGGAACGTGTTCTTTTGCTGGTGATTGAGTTTGTACCACTGGCGTATAAGCGGGTATGTGTGGCTCTTTGCTAACGGCATTGTTATAGTGAATCACAATTGCTTTAATCTGATCACTTAAGGATAGTTGTGAAGGACCATAACCAGTGTTAGCTTCAATTGCTTGAACCAAAAGAGCATACTGTTTGGGGTGAAAACGAAGCTTTGCCGCCATTGTTTGGTCGCTGGGTTTTACAACTGCAATTTGTCCCGTTTTGACATAACCATATAAATCAACTGTTAGGGCAAACAGTTCCCTGAGAAAGTGAACAAAGTTAATCCCTTGACTTTCAAAGTTGTCAATTAAGTGAAAGGATTGTTTGAGATCACCACTTAAAACTGCTTCAATAAAACCAAACTTTTGTTCCTTGTCAAGCAGGTTAAAGGTCTTTTCAACATCAGCTAAACTAATGGTTTTACTTTCACTAAAGTTACTAATTTGGTCCAACAAGCTCAACCCATCACGCAACGAACCTTGCGCTAAATCAGCTAGTTTGACCAAAGCATCAGTAGTAATCTTAATGCTTTCCTTAGCCGCTACTTCCGCTAACCGTTGCTGGATGAGATCGTTAGTAATTTGTTTAAAGAAGAAGCTTTGGCAACGCGACAAGATCGTGATCGGAATTTTGTTAAATTCGGTGGTGGCAAAAATAAACAAGACATAATCGGGTGCTTCTTCTAAGGTCTTTAAAAGCCCACTCCAAGATTGGGGGGTGAGCATGTGGGCTTCATCCAAAATGTAAACCTTCTTTTTGAACTTAAAGGGTAGGTTAAAGACATTTTCCGCTAGCTCCCTAATGTCGTTAATCCCGTTTTTGGAAGCAGCATCAATTTCAAACACATCAATCGCACTGTTGCTGTTAATCGCTTGGCATGCCTCACACTGGTTGCAAACATCGCCGTTCCAGTTTAAACAGTTAATGGCTTTGGCAATAATCTTGGCAAAGGTCGTTTTTCCCGTACCCCGCTCCCCCGCAAAGATGTAACCGTGGGGTAATTGGTCTTGGGTAATGGCATTAACAATAATGCGTTTAATGCTGTCCTGACCGACAGTATCGCTAAACTTGGTGGGACGGTATTTCTGGTAAAGGACCTTGCGCATGTTTAAGCTTACTTTAAGATTTTAGCGAGATACTGGGCAGTGTAGGACTTATCGACCTGATTTAACAGTTGTTCTGGTGTGCCTTGGGCAATAATTTGTCCACCTTTTTCCCCACCTTCAGGACCCAAATCAATGATGTAGTCCGCCATCTTAATAATATCTAGGTTGTGCTCAATAATAATGACACTATCACCGTTTTTAATAATCCGTTGGATCACCGTTAACAGCTTATTAATGTCTTCTATGTGTAGTCCCGTTGAAGGTTCATCCAGGACGTACAAGGTTTTACCAGTTGCCTTTTTCTGGAGAAACTTGGCTAGTTTAATTCGTTGTGCTTCCCCACCTGACAAGAAGGTAACATTAAGACCTAACTGGAGGTATTCCAGACCGACATCACAGAGTAGTTTTAACTTTCTCGCAATATTCGGAATTGCTTTAAAAAAATCATAAGCTTCCTGACATGACATCTGTAACACATCAAAGATCGATTTGCCTAAGTACTTAACTTCCAGGGTTTGGGGATTGTACTTCTTGCCATCACACATTTCACACGTCACATAAACATCGGGTAAAAAGTGCATTTCAATCCGAATGACCCCATCACCAAAACACTTATCACAGCGCCCACCGGCTACATTAAAGGAAAAGCGCGAGTTAGTGTAACCCCGAGCCTTGGCTTCCTTAGTGTTGGCAAACAACTCCCTAATGTCATCAAATACACTGACATAGGTAGCGGGGTTAGAGCGGGGAGTGCGACCGATCGGATCTTGCGACACCACTACCACCTTATCAATGTGGTTGACCCCTATGATTTCCTTATAGGGTGCAGGTTTAACATTCTTATAGTTCACCAAGCGTTCCAAGATCGGTACTAAGGTTTGGTTAATTAAGGTTGATTTCCCTGAACCCGATACCCCGGTCACTAGTACTAATTTATTTAAGGGAATAGTGACATTAATGTTCTTTAAGTTGTTGCCCTTGGCTCCTTTTAAGGTTAAGGTGCGACCATTACCAGCATGCCGGGTTTTTGGTACCTCAATTTGCTTTTTGCCACTGAGATACTGTCCCGTTAAGGAGTCAGGATTTTCCATTACCTCTAGCGGTGTCCCAGCAGCTACTACTTCCCCACCTTGGTTACCCGCTTTAGGACCAATATCAATGAGGTAATCAGCTGCTAGCATGGTTTCACTGTCGTGTTCTACCACGAGTAAGGTGTTGCCCAAATCCCGCATTACCATCATCGTGTTAATTAAGCGATCATTGTCCTTTTGGTGTAGCCCAATTGAGGGTTCATCCAAGACATAAAGGACGCCAGTGAGTTGCGAACCAATTTGGGTGGCTAAGCGAATCCGTTGTGCTTCACCACCTGACAGGGTAGAAGCCCGGCGCGCTAAGTTAAGGTATTCTAAGCCCACATTCTTCAAGAAGGATAAACGGTTAATAATCTCTTTAAGAGCTAACTCACCAATCTTCTTCTGTTCTTGATTCAGTTCTATTTGCAACAAGAATTCAATTGCTTTATCAATCGAGAGTTCCGTAAAGCTAATAATGTCCAACCCACCTAACTTGACACTTAAAGAATTAGTAGTAAGCTTCTTGCCCTGGCACTTCTTACAGGTAATTTCAGACATGTAAGCCGAGTACCATTCACGACTGGCTTGACTACTAGTCTCTAAGTGACGGCGTTTAATTAAGTGAGCCACACCCTCATAGTGTTCAAAGCGCACATTTTTGGCACCTGAGTTTGATACTGTTTTAATCTCAATTGGTTCATCACTACCCTCCAAGATCATCCGTACTAAATCAGTATCCATTTGACCTAAGGGTTGGTCTAGGGGAATTTGATAGTGGTTAATTAAAGACAAGAAGCGCTGTCAATCTAGTGATGTACCGTGCACTATGTTCTTAAAGATGTCAATACCACCTTCATTGATGGACAACAACGGATTGGGAATAATCTTTTCCACATCCGGTTCATAACTAAAACCTAAACCCTTACAGTACTCACACGAACCTAAAGGGGAGTTAAAGGAGAATAAGCGTGGCTCTAGTTCACTAATTGAAAAACCACACTGATCACAACCGTGGTTCTTATTAAAACTAAGTATCTTACCATCTTCCAACAACACTTCAATCCGACCCTCAGTTAGTTTAATCACACCTTCAATTGCATCAACAATCCGACCCAAGGTTTGCTTGTCTTGGTTAATGACAATCCGGTCCACTACCACACTCAAGTTGTGCTTGGTGTTCTTGTCTAACTTAATTTCTTCATCTAAGGTATAGACCTGACCATCAACCCAAACCCGGAGAAAGCCTAACTGTTGGTATTTAGCAAACTCATTAGCAAAGGTACCGCGTTGGTTTTTAATGGTAGGAGCTAAGATTTGAATCCGTGATTTAACCGGTAAGTTAAAGATCTGGTTGGCAATTTGGTTAACGGTTTGGGTTTGAATGGCACCATGACCATTAGGACAAAAGGGTATACCAATCCGCGCTCACAGTAAACGGAGGTAATCGTAAATTTCCGTTACTGTTCCCACAGTGGAGCGGGGATTGTGTGAAGTGGTTTTTTGGTCAATTGAAATGGCTGGGGATAACCCTTCAATCGAATCTACATCCGGTTTATCACTGTTACCGAGAAACTGTCTGGCATATGACGAGAGTGATTCCAAATACCGACGGCGGCCTTCCGCGTAAATGGTGTTAAAGGCAAGTGATGATTTACCCGAACCCGACAACCCCGTAATAACGACAAACTGGTTTTTGGGAATATCGATGTTTACATTCTTCAGGTTGTTTTCACGCGCGCCCTTAACGCGAATAAAATCATTAGTTTTTAGCTCTGGTTTCAAAGTTAATTGTTTGGGTGAAAAAATCCTTTACGAGGTTATTGTGCGGATCGTTAAATAAATATGCAATGCCTTGTTGGTTTTGGTGGGCAATTAACAAACCACGGGTAAAGTTATAAGTGTGTTTGTTAAAGTTGACAAAGGAGTCGACCAACACCAACTGGGGGTGTAACAATAGCTTAAATAACAGGTAAAGTTTAAAGCGTTGCTTGGTGTTAATTTTATTTAAATCGCTAAAGATCATTCAGCTCGGAATACCGTTTTTAAAGGTGTTATCCAACAGCATTTTGAGGTTAGCAGTACGGTTTAGTTTTTTGGGATTTTGCCGGCACTTTTTGAGACAAAAACTAATGACATTCACCATCGCGTTTAAGAGATTAAAGTGCAGCTTATTCGATGCCTGTAAACGGTTTAAATCACACTCTTCTGAATCCACAAAGAAGGTTTTGCGTACCACGTTATTGACTTTACTAATTTGAGTATTGAGCAGCTTATTTAAAGTGCCATAATTTTTTAGCAGTGCTTTAATTTGCTGTTGCTTTTGCTTAATTTCTTTTTTGTACAACCACTGGATTAAACGAATGTCTTGTAAAAGGAGGTGGTAGTGTGCCCGTCACTGTTTGAGCTGGGTTTTTAAGTTAAGTTGGTTTATGGTAGTGGTGTTTGCTTCTGTTAAAGTAGTGGCATTACGATTAGGTAACAACAAGTAAGCTTGAGGGTGGTGCAAGTTGGCTTCGAAGTATTTATTAACTGTTTTATTCACCAGTAACTTTCACTTCGGGGAATTTCAATCGCGGTTTAAGTTGGCCTTTAAGCTGGCAAACTGAGTTTGATTCCGACCTTGGTTAAAGACAAAGTCAACCACACCATGGGTAATAAACTGGTATTTGGTAAAGAAGTGTTGTGTTTGTTTGTGCAAGTTAGTTCATAAAGCACGGCACACAAAGTAGAGTTGGTTCAATTCGTATTTCGTTCAATCTAATTTTTCGTAAGTAGTTAAGAGTTGTTCAAAACAGCGTTCTTGAACAATATTCATTAGGTTAAACGGATCTAAGGTGTGTTTTAACTTAGTGTAGTTAAGCGCTGCTTTAACTGTTTGGATAAAACCCTTAAAAACCGCCTTTTGGTTTACAACCGAATAGCTTTGGCTAACCTCACCCTTATACTGGTTTTGGTTTAACAGAACGTTTTGCTTTAACTCATTGACCAGCTTGTTGTGTTGCTCACAGCGATTTAAGCCCTTAGCGTATTCTTGCAAAAAAGCTAAACGAGATTTAATTAAAAAACCAATCACCGCATCATGAGTCAAAACAATTGGCTTAGTTAACTTTTCCAATTCAAAGCTAATTGCTTGGTATTTTCAGTTAAGTGATTGACCTGGTTTTAGCTGCAAAATTTGCTGGTACAAGCGCTGGATGTTGCTCATTAACTGTTCTACTAGGCGCTCACTTTCAGCAATTGTGCTCACTAACAAATCGGGATTAAGTAAGCGCATTTGTTTCAGTTCCTTTAACAGTCAAATTTTGATTTGGATTCGCTTGGCTACAATCCGTTTTTCCAAAATCCGGGTTTTGTAAAAGTTAAAGATACTCTTGAACTTAATCTTGGTTTGACTCCACCACAAGGACGACATAAAGCGGTTAATCAGCTGCACCGAATTGCGCTGCTGTTTTTTGAAGGCACGCTTAAGCTGATTGGCTTGCTGTTGTGCACGGGTAATTTGCAAATTTTCAAAGTAGGTATTAACCCCGTGTTTGGTTTCATAGCTTTGAACTTTTAAATTGTGCAGTAAACGTTCTCGTTGTCTTGTAAGCGCATAGTACTGTTCAAAAAAACTTTTAGTTAAGTGATCAGGTTGTTCAAAGAAGCTGTTAAAGAAGTGGGTGCTAAATTCCACTAGGTCACGTTCATGGTCTTCCAATAAACCAACAAAGTTGTTGTACTCAAACTGGATCTGGTTAAAGTACACCTTATTGACAAACTTAGTGGTGAAGAACTGACTAAGTGTTTCACTCGTTAACTTATTGGTGTTATCTAGTTCAAACACCACAAACTGGCTTAACTTCTTGAGTGCATTAAAGCCCAAAAATCAGCGCAACTCATTGGGAATTTGCAGTTTTTCAATTAAACGCTGTCGTTTTTTAGGACAAGTACTTTGTCTTAAAAAATCATGAATAGTTTGGTGAAACGGATGGCTTTGTTGCACCGCGGCCAGTTCAATTTTTTCACACAAGTTGGTTTTAACGCGATGCAGCGGAATATAGTTGTGTTGATCCGTTCACAAAAAACCGGAATGGGGCGCTTTTTTGAGTTGTAAAACACTCGACAACGTGTTTCACTGTGCCTTATTGAGGTTATTAACGAGGTAGTTACCGTATTTATGGATGCTGAGGTTGGTGGTTCAGAAGTAGTTAATGTCCAAAAAGGAGTTATGGACCATTAACAAGGACAAATTAACTCACGGTTCTTTCTTAGTGGTAGTGGAAATCTGTTTCAGCATCCCTTAGTCTTATTGATTTTAGTACTAGGGTCATCCCAGTAACAACTAAATAGAAATAATAGTTGACAGGATTAGTGGTGCTTTAGCAGTCATTTTGGTAAATAAATTCATGACAGCTCGCTTAATAGAAACCTTGAGTTCCTTCGATGTGTCTTTGCCTAAACGTCGCTTAATTGTCGTAAAGTCCTTAATGTCGTTAATTTGTTTGCGAATTAACTCTTCCATTTTGGTCTTGAGTTTAACCTGATTGTTACTATCTAGGCTAACTCCTAAAAAGGAATAGGTAATTTCGTTTAGGAACTCACTCTTCTTGGAGTCATAGTAGATGATGATGGTAACCACACCCGCTTCAGACATCTGATCACGCTCAAACATGATGCTGGCCCCTATTTCCTGCAAACCAGCGGAGTCAACACACTTAGGGTTGAGCTTTAATTCCCGTTTTTTGTTGTCAATTTGCTTGTGGTTAATGGCTAGGACTTCACCATTAAAGGGAATTAAGACCTGACTTTGTTCCACACCAGCTTGCTTCATTACCATGGTAAAGTTAATGAAGTCACGGTACAGACCGGATGTGGGAATAATGTACTTAGGTTTTAAGGAAGTTACTAAAAACTTCATGTCTTCATCCGATGCATTGATCGACAAGATTTCCCGACCCAAGTTGTAGTAACTCACCTCATTACGCGCGACATCGTCCAAGATTTGTGCTTCCAACTCTTCATACCCAGCTATGCGGGGAATCATGAAGATAAAGCTATCGGTCTTGCGATACCGTACCCGTTCATCCTCATTAGTACCAATCTTAAACAGCTTGGCATACAACTTATCTGGTGGCGCGGTTAAAACAACAATGGCGTTTGTGGAGTTGTTAATCTCTTCAATCGAAATAGTGTTTAAGTGGGTGTTGTTAAACAGCTTTTGGCGCACAATCGCGTTGAAGAGGTGGACAAATGAATGGGAGTAGATGACAAAGGGGCGGTTTTGCATCCGTGCAATTTGGGCCAAGGTCATCACACTGTAAGCGTTGGAATCATAACAAGCAGCAAATACCCGACCCTTAGCGGAGGCAATAATGCGGTTGAGTTGTTCCAAGGATTTGTGCTTAGGGGTTGTAAAACCAGTGTTACGTCCCACTAGACCAACTCCAGTAATTAACAAAAGGGTCTTGTTCGCTACTTGGGGAATAATCTGATTGAGTTGGTTTTCAAAGGCGATGTTCTTATCATTGAGCACAATGAAGTCATCGACATAGACAATGTACCCATCATCAGTGTGGAGCGCAAAGCCAAACGAGGAAGGAATCGAACTCGATACCTTAAAGGGCGTAATGTTATGGTGCCCGATCTTGACCGTTTCTAACGGTTTAAGCTCGTGGATTTCCAACTCGTCATGGGGAATATTGAGCTTGTTTTCGTGGATCTTAGTTTTAATAACCACAGCGCCAATCGTACTGGTGTAAATCGGAAAGAAACCAACGGTGTGGAATAAGAACTCCAGCGAACCAATGTTTTCCGTTACTGGATTACCGATAAAGATGCCCTTAATGCGGGCCTGGTTTTCCTGGATCCAACTAAAGTCAGGGATGATCTTTTTGACCCCTAAAACAGCGGTTGTTGGTGTCAAACTACCAACGTTAAAAATAAAAACGTCGTTATTGATTTCGAGAACAAAACAATTCTTGCCCCTCTCGTCCTGACCACCGAAGGCAAAGAAGTTAATTTTAGCCATGAAACTAACCCCGGAGATTTAAGGTTTTAACTAAATTTCTGTAAGCCTCTAAATCGTTGTGCTTTAAGTACTTCAACAAGCGCTTGCGCTTTGATACTTTCGCGTACAAACCACGTTTGGAAATAAAGTCCTTCTTGTTAGCTAGTAAGTGGTCGGTGAGCTGTTTAATTTGGCTCGTTAAAAGGGACACTTGTACCTGAATACTACCCACGTCCTTGTCGTGGAGTTGTGCGGATTTAATGATCCCGTTTTTATCAATTTGCATGAATTAATACTTGTTGAATTATAACAAAATAAGAAATTAGCCCAGTCAAGGGGTGAACCCCACAAAATCAGCCCCCAAACTTATAATAAATAAACCAAAACAACCACCTTAAGTGGGCTGTTAACTAATTAATTACTAATAACTATCAAAGTCATCTATGGACTCAACTTTTAATGAACTAGGCGTCTCTCCTGCCTTAATTGCCACACTCAAAGATAACAACATTAACCAACCAACTACGATTCAACAACTGGCTATTCCCCAGTTTCTCCAACATCAAAACCTGATTGTCCACTCCCCCACCGGCACTGGTAAAACCGCTGTTTTTGCCATTCCGGTCATTGAAACACTCCTTAAAAAACCAAGTAAAGGTACGACCCAAACATTGGTAGTTGCACCTACCCGTGAACTGGCCGAACAGATTAAAACCACCTTTATTAACTTTGCTAAACACACCCATTTAAAGGTAGTGAGCTTAATTGGTGGCATCCCCATTTGACAACAACTCAAACAACTAGAAAACCAACCAGAAATAGTTGTGGGCACTATGGGGCGGGTGATGGACCTGTTAGAACGGGGTGTAATTAAGTTTGAACACCTAGAACACCTCATTATTGACGAGGTTGACCTAATGCTCGACCGTGGTTTTAAGAGAAAACTGTTTGATCTGTTAAGTCGCATTGAAAAGTTTGAACAAATTGCGGTTTATTCTGCTAGTTACAATGAAGAGACAATTGAAACAGCTAAACAAATTACCAAGAATGGTATTTTCCTAGCAGCACCAGAATTAAAACAAAATGCCCCTGAACCAGATAACAAACTCATTGATCAATTTGTTTGTTATCTTTTTTCTAACCGGAAGAAACAAGCCCTCTATTCTTTGGTATCGCAAACTAGAGCGAAGTCGATTATTGTTTTTTGTGACACCAAGAAGTTAGTTGATGAGCTGTGCATCTTCTTGCGCAAGAATGATGTCAAAACTTATCCACTTCACGGGGACAAAGCGCAGTTCATTCGCGAACGCAACTTAAAGCTGTTTGCTAACACAACTGCTCCTATAGTATTAGTAACAACTGATCTAATCGGTCGTGGTATCCATGTGGAAGGGGTGGACATGGTAGTCAACTATTCCGCTTGTGTAAACTTTGAAACTTATTTGCACCGGATGGGTCGCACAGGACGGAACAACCACAAGGGCTCATGTATTACCTTCTGTACCTCACACGAAAAGCAAGCATTTTTAAAGTTATTAGAACAAGTTAACGACAAGCGCATTAGCCCTCTCCGCCCCATGCGCTTAAGGTTAATTCCCTTGAAGTGCAAAACCCAACCCAAGAAGGGGAAACTTTCCTTACAGTCAGTGCAAAAGATTTACGTCAATCCTCGTTCCAATGGCACCTTTAAACGCGTACCTTTAGCGGGGGATTTGTTTAAAAGCCGGATGCGTCAACCTGAGCGGGACATGCAAAAGAACAAATTGCATGACAGTGATTGACAAAGCAATATGTAAGCTTTGTTACAATTATTTGATATGGCAAAAAAAGACCAACTTACTTTAAGAGGGCCTTTGTATGGCAACAATCGTTCCCACTCCAAGACTATTACGCGCAGAAAGTGGAATGTTAACCTCCAACCATGCAAGGTCAAAACTGCTGATGGCAAGACCACCAGAATCTTAGTTTCTACCAGAACACTGCGCACCTTAAAGAAACACAACCGCCTCTCTTAGTGGCGCGCTAGAGTTAAATTAAACTTAAATCCATATAGTAAATATGGACAAAAAGTACGACATCACTGCTGTTTTAAACGAAGACAGCTCCATGACTGCCATCAGTGATCAGTTTCAAATTACACTCGATGCCCGTCCCAAGCACACCGCTAAGGGTTTTGGTCCTTTAGCTGCTCTGCTCTCTGGTTTAGCTGCTTGTGAATTGGCAACCGCTAACTTGATGGCACCAGCTAAAATGATTACCATCAATAAACTGTTGATGAATGTCACTGGTTCACGCTCAACCAACCCCACCGATGGTTATTTTGGTTTAAGGGAAATTAACCTGCACTGGGAAATCCACTCCCCTAACAGTGAAACGGAAATCAAGGAGTTCATTGACTTTGTTTCCAAACGCTGCCCAGCGCACAATACGCTCCAAGGTGTGAGTCAACTCAAGATTAACGTTAATGTAACTTTAGTGCATTAATTGGGTAAAAAACGAGCGAAAAATGCTGAAAAAATACCTGAGTCTTTCAGGTATTTTTTTATGCAAAATTTGCCCTAAAAAAAGAGTAAATTTGAGAAATTGATCCGTTGTTCATTCCGGGATAATTTTCCCGTATGAAACTACAAATTTTTAATACAAAGCTAGGATTACTAGCAGCTAAGATTTACTGAAAGTCCTGGAGACACCTAGGACTGTCTGAAGATGAAATTGTGTCAATTGCGCTCCACGCCGAGCATGACTCCAAGAAGCGCTATGACCCCAAATACGGTTTGAGCTTTGAGACCTACTTGAAGTTAAATGGTGCTAACTTCATTAAGTCGAGTTACCGTAGCTTAATGAATAAGGTTGATGACTGAATTGGCTTTAAGGACATGAAAACACTCGAGGAACAAAACACAGTCTCTTACACTCCTGAAAACTATTTAAGAAGTGTAGAATTCCGTGAAATGGTGCACTTAGCGTTTAAGAAAGCCAAGAATGAGGATGAACGTCAGGTGTTTGCGCTTTATGTAAAGGGTTACAAAAATTTAGAGATTGCTAAAAAACTCAACCTAACGACCCGTAGAGTGCGCTACCTAATTTCAATCTTTAAGGATCACATTAAGGTATTGACTAAACGGTATGGTTACTAGATGAAGATATCCTTCTTCTTGAAGAATTTTTCCACTAGCTTTTCCACCTCCTTGGCGTTCGCTAAGGTTAATGCTTTTTGTGCTAATGTCTGACACTCTGTGTAATTGAGCTTAGCGATAATCATCCGCGCCTTAAACATGGAACTAGCACTCATGGAAAACTCTTTGAGACCCAAACCTAAAAGTAGGGGAATCGCTAACGGTTCACCGGCCATCTCACCACACATTCCCGTTCAAATATCATTCACTTTACCCCCTTCAATGGTGAGGTAAATGAGTTTTAATAAAGCTGGGTTTAAAGGTTGGTAGAGATAACTAACATTCTTATTCATCCGGTCAGCCGCAAAGCTGTACTGGATCAGGTCATTGGTACCAATGGAGAAGAAATCCACGTGGTGACTTAAGGTGTTAGCCGCTAAAACAGCAGCGGGGATTTCGATCATAATCCCGAGCTTAAACTGTTTATTGCCACCCAACTCCTGGTGCACCTGGTTTAACAGTTGCTTGGCTTGCAACAGTTCATCCAAGGTAGCGACCATCGGGAACATAATGCCCAACTGACCGTGATCAGCAGCACGTAGTAAAGCCCGCAATTGTGTTTTAAAGATGTCCTGTTTGTCTAAGGTTAAACGCAAAGCGCGGTAGCCCAAGAAGGGGTTATCTTCGTGAGGAAACTCATAGTAATTAAGCTTCTTGTCCCCACCAATGTCTAGGGTGCGGATAATAACTAAATCACCGTGCGCTTGTTCTAACACTGCTTTGTAAGCCTGGTACTGCGTTTCTTCATCGGGTCATTCTTGACTGTTCATGTAGAGGAACTCAGTGCGGAATAAACCAACCCCGTTGGTATTGTACTGACATGCCAATTCCATGTCTTTCACATTACCAATGTTAGCAGCCACCACAACCGCATGACCATCTAAAGTCACGGTTTCCTTGTTGGTGTACTGCTTGAGTTCTTGTTGGTATTTTTCTTCCAACGCTTTTTGTTTCTTCCACTCGGTAGTGTCCTTGGAGTTTAGGTCTAAGCCAGCTATTCCTTTGCGCCCATCAACCCCAACTATTTGACCGTCTTTGACCTTAGTAGTAATGCCCTTAATGCCCACCACTGCGGGAATTTCCAGTGAACGCGCCATAATGGCAGCGTGACTGGTACGACCACCTATTTCGGTTAAAAAACCCTTGACGTATTTTTTGTTAAGGGTAGCAGTTTGACTCGGCGTTAAGTCATGGGCCACAATAATGACATCACTCTTAATTTTGACCAGGTCATGTGGTTTTTGTCCGGTTAAATAAGCTAGTAAGCGTTGGCGCAAGTCCAAAATGTCTGCAGCACGTTCCTGGAAATACTTGTCCTGCATTTGGCTAAACATGGTAGCCGTTTGGCTAAAGACAGTGTCCACGGCTATAACAGGATGAACATTTTGCGCTAACTGTTGTTCTAGTTGCTCGGTGATCGTGGGATCGTTGAGCATTTGAATGTGCGCATCAAAGATCATGCCCGCTTCAGTGTTAATGTTGTTAACGGTAATTTCCTTAATTTCCTCTAAGTCCTTAACGGCTTTTTTGAAAGCTGAGTCCAAAAGTCTTTTGGCCTGCGCCTTCGTTAATTGGTGCTTGAGGTATTTGTTAACAGCGAATTCCGGTGTTTTAACAAGGAAGGCTTTGGCTAGGGCCATGCCATCAGATACACCGATACCAGATAGTTTTTTCATTTGTTTACTTTTTACTTATTAAAAGGGAGTTTGCGGTCATTTCTGCGGGCTGTTTGAGCCCTAAGTAAGCCAAAATGGTTGGGGCAATATTGGCTAGACTAGCACTGTCTTGTTGTAAGTCAATGTTTGTATCAGTACATACAAACGGCACCTTGTTGACAGTGTGTTTGGTAACTGGGTTATTGTTGCTGTCAATCATCTCTTCCGCGTTACCATGGTCAGCAGTTAAGAACAGGGTAATGTGGTTAGCCTTGCAGAAATCAATAATGCGCTGTATTTGCACATCCAAGGCCTCTAAGCCCTGCACACAAGCGGCATAGTTACCAGTATGACCTACCATATCAGGGTTGGCAAAGTTCAACACCGTCAAATCGAACTGGTTAATTTGATTTAGTAATTGATCAGTTATCCCCTTACATGCCATTTCCGGTGCTAAGTCATACGTAGCCACCTTTAACGAAGGTACCATGCACTTAGTTTCATTGGGTAGATCCACTTCCACTCCACCATCAAAGAAGAAGGTTACATGGGCGTACTTTTCCGTTTCTGCAATTCGCAGTTGCTTTAACTTATTGTGCGCAATGACTTCCCCAAAAGTATTGGGAATAGTTTCGGGCGGAAAGACCACGGCACTTGGTTTAATCCCCTCATAGTTCATCATCGTGACAAAGAAGAGGTTGTACTTTCGTCCTGCTTTAGGGGTGTAGTCATAGTAATCTGTTTGAAAGAGCATGTGTGACATTTGCCGCGCACGATCAGGACGGAAGTTAAAGAAGATCACACTGTCATGATCTTTGAGCGCAAACTGCTCTTTATCTACATTACCATTGACAGCCGGGTACAAAAACTCATCGGTTACCTTTTGGTCATAGGCACTTTGAACATAAGCTACCGGATCAGTAAAGCTAGCCTTACTGTTACCCAAAATGGCATTATAAGCAATCTCTTCTCTGTCCCAGCGCTGGTCACGATCCATACCATAGTAACGCCCACCTAAGGTACCAATGATGACATTGTGGTAATCCTTTAAGAACACCATGAAGGCTTCAAGGTCACTCTTCATCGTGCAAGGTGCTACATCCCGCCCATCACCAAAGAGGTGGAGCACCATTTTGACCTGCGGTGCTGCTAGTTTAATCAAGGCATACAAATGGTCCATGTGACTGTGTACCCCGCCATGGGAAAACAGACCAATTAAGTGCAACTTAGCATTGTTAGCCTTGGCCCTAGCAATCGCCTCTAGTAACACCTGGTTGTGGTGAAAGGCCCCGGTTTTAATGTTTTGGTTAATTAACGATAAACCAGTGTAAACAATTCGACCAGCCCCAATATTGAGGTGACCCACTTCCGAGTTACCGATTTGTCCTTGTGGTAGACCAACGGCTTCCCCCGAAGCTTCTAACATGACACAGGGATAATCCTTAATCAAACTGTCTAAAGCTGGAGTTTTAGCATGGTACACCGCATTACCGTGTTGCTTATTACTAATACCATAACCATCCAAAATGGCTAGTAAGACCTTCTTATGCATATACTTGTGCCATTACTAAAAAGTCGTTGACATCCAAAGATGCCTTGCCCACTAAAAAGCCATCAATTTGCTCGGTTTGTGCTAACTCCTTAATGTTGTCCTTAGCAACCGAACCACCATAGAGAATCGAGGTTTGTTGGGCAATTTCCTTGTTGTACAGTTCACTAAGGTACACGCGAATTTGGGCAATTGTGTTATTAGCAATTTCCGGTGTAGCGGTTTTACCCGTACCAATTGCTCAAATTGGTTCGTAAGCAATCACTAACTGTTTGAGTAAGCTTAGGTCAATGCCTTGCAAGACTTGGGTTAAGTCTTCTTTGAGAAAACTAATTGGTTGACCTTCCGTTTCACCAATACAGAGCACGACTAACATTCCAGCTTGTAAACAGGCTTTTAGTTTTTGATTAATAACTTGCGCTGTTTCGTTAAAGTACTTTCGCCGTTCCGAGTGACCAATAATGACACTGTTGACCTTAATGTCTTGCAGTTGGGTATAGCTCACCGTACCAGTGTAAGAACCACTAGCAATAAAGTTAGCGTCTTGGGCTAACAGTAAGAGGTTATTAGGCAAGACTTTGTTTACCTCGGTTAAGTGGACGTGGACTGGGGCAATACCCAAAACCGCCTTAGTTTTAGCAGGGTGCTGTTTAAATTGCTCCACAAAGGCCAACGCTTGGTGTAAATCCTTATTTGTCTTTCAGTTACCAATTAGGTATTTCGTACGCATACGTTAAAGATTTTATAAGTTTTATTGATTAACCCCAGCGGGAAATACCTAATTAATTTTCCTGGGCTTTAACTGGGTCTTTTACTGGTGGGACTACTTTATCAGGTGCTTTTCTTTCATAGATATAACCATCCAACTTTTTAATGAAGGTAATTGAGAACAGAGCATCCGGATCGACTCGTCTAACAGCTTCTAAAAGTTCGGCCGTTTTCGTAAACAAACAGTTTGTAACTAACACCTGGTTCTTTTGGCGCGAGTACCCCCCTTCCACTTCGAACATAGTGACCGCAAAGGACTGGTTACTGCTCAACAAGTAGTTGCGCATTTGTTCCATGTGTTTACCATACACTTCCACTTTGACAAACTGGTATTTAGGAAAGAAGTAGGAGGTAAAGATCCCTAAGATAATGTTCATGAAAATCGTAAACACTAAGTTAGGGGACAAGAAAAAAGCCACTCCAAACGGTTTTTGTCTATCTTCTTGAAAGCCTTGTGCTAGTAAGCTACCGGTAAGGTAAGTGCCAATGGTGTAACCGATCAAGAAACTAAGGGTGTTAACAATAAACAAAATACCACCAATGTCCTTGTGTTTCTTTTCCGAATACCAGAAGGCCAAAAAGTCCAACCCACCACTCGATGCATCAATAATTAGGATAACTGAGTAAAACACAGCTTGAAGAAAACCCCAGATCAGCGCATAGAACATTAACGAAATTTGCTTTTCAGCGGTTTGTTCCCAAAAGATCAGTTGCACCCCTTGGTTGTTAATCGCTTGTTCTAAGCCACCGTTGGCCTTAGTAAGTTCAGTTAAATTAGCAAACAAGAAGAAGTTTTCCACGCCCGGAATGTAAGAGAAGGCAAACCCAAAAACGTTGGAGACTACCACAAAGTAAAGGGTTAACAAGGTAAACTTTTTGGAAATCTTGTACCAACCCAAAACAAATAAGGGAATGTTAATCAGAATTTGACTCAACCAGAAGATGACGTTGTAAATGGTGTCAGCATCCACACTGACCTTGTTCGACCTAATTAAATAATCCGCTAACCGTGCTAAGCCCTGACTAATGGAAGCAATGCCAATGTCATATAATCCAGAAAACTGGACAAACACAACTCCTAACAAGCCCCAAAAGAAGGCAATCACGGAGAGAATTAACAGCTGTAAGTACAGCTTTTTCACCTTGTAAATTGCCTGGAACTTCAGAAACGATCCCGAAAGGTGTACCCGTACCAGATTGCCCTGAATTTTGACGTTTTTATCTTGCATATTCCAAGTCCTCCTTTTTCATCATTTTGCCAATTGCTAACAGGCTTAAATTTAATATTTATTAGGTACAAAAATCGATTACATTAATGGCAACAAAAATAGAGCTCATAAAAGAACTACGTAAAACAACACAAGCGAGCATGATGGACTGTAAAAAGGCTTTGGAACAAAATAACGATGATCTCGAAAAGGCCATTAAGTGATTGAGAGAAAACGGAATTGTTAAATCTGCTAAGAAATTAGGCAAAGTAGCCGCTGATGGTTGCATTGTGTTACACAGCGATCACCACAAGGCAGTGATGGTGGAAATTAATTCTCAAACGGACTTTGTAGCGCGTAGTCAAGAGTTAACGGACTTTGCGCAATTAATGATTAGTGAAGTCTTTAAAAAGGCTACCCCTACCACTACTATTGAAGAAGTAACCCAGTACGAACTCCAAGGCAAAGAAAAGGTAGCAGAACGTTTGGCTTTAGTCGCTTCCAAAACTGATGAAAAGATTGTGTTGCGCCGCTTAATGGTGTTTGAAAGCAAAACTAACCACATCTTTAGCTACCTCCACGCTAACAAACGGATTGGCGTAATCTTGGAAGTGGAAGGCAAGTTTGATGAACAAGATGGTAAACACTTAGCAATGCACATTGCCGCTAACTCACCGCAGTTCATTGATCAGGACAATGTGGATCAAACATGGTTAGCTAACGAAACCAGCATCATTAAGTCCCAAGCCAAACTAGAAGTCCAAGACAATCCGAAGAAAGCAGCGTTCTTAGACAAAACTATTGCTGGACGCGTGAACAAACTCTTAATTGACATTTGTCTGGTAAACCAAAAGTACTTGGTCGATGAATCCAAAACAGTTGGTCAATTCTTAAAGGAAAAGAACAGTAAAGTAATCCATTTTGTGCGCTTTGAAGTTGGTGAAGGCATTGTCAAAGAAGCAGTTGACTTCGCTAGTGAAGTAAGTGCGCAAATGAAAAAATAAGATGCGCCTAAAGATCCTAATCAAACTAAGCGGTGCTGGCATGAGCGCTGATAGTTCCGAACCGTTTTCCAACCAATTTTTAGAAACTGTCATCGCTCAACTAAAACAACTAGTACCCAACTACCAAATAGGCATTGTGATTGGTGGGGGCAACATTATGCGCGGTAAGTCTTGTAGTGACTACAACATTACCGAAATAGCAGGGCACCACTTGGGCATTATGGCCACGGTTATTAACGGCGCTTTTTTAAAGGCTAAGTTTGATAGTCATAAACTTAACAGCACCCTGTTGAGTGCAGTGAGCTGTCCTAGTTTAGCGACCCACATTGTGTCCCAGGCCACGATTGATGATGCCTTTAAAAACCATGACATTGTCATTTTTGCGGGCGGTACTGGTAACCCGTACTTTTCGACTGATACCGCTGTGGCGTTGCGTGCTACCCAAATGCAAGCCGATATTATTCTAATTGGCAAGAATGGTGTTGATGGGGTTTACACTGCGGATCCTAAAAAGGATAAGCATGCTAAGTTTTTAGCTAGCTTAACTTACGCTGAAGCGATTAAGAATGACCTGCAAATCATGGACATTACCGCCTTTACAATGTGTAAGGAAAATAACTTAAAGGTGATCATATTTAACATCAATGCCGAGCATGCCATCATTAAGGCATTGAGCAAACAAGGTAAATACACCTTAATAGAAAAATAAGCAACTGTTTTTTAGATTAATAACATAATTAAAAGCTGGATCGCTGTTTAATCTCCTGCAGCTTTCCAGCTTTTTTATTAGCCATTTTCAAAATAACTTTCTATGCGCTACTTAGACCTCAATATTAAAAGCATCTTAGCTGATTGAGAAATTGCTGATGCGATCCGTGAATTGATTGCCAATGCGATTGATGAACACCGCTTAAGCAACACCGCTTTCCCCGTAATTGAATTGCAAAAAGGCTTTCTAAACTCTTCTTTAGTGATTAAAGATTATGGTAGGGGTATTAAGTCCAATCACTTTATCCAAAACGAAAGTAGGGAAAAGGTTCAAAGTGAAAAAACTATTGGTAAGTTTGGCATTGGTCTCAAAGATGCCATAGCTGTTCTTTTCCGTCATAATGTCAAAGTTAGCTTTACCAGTAGTGAAGGCACTTTTACCCCTGTTGAGCGAATGAAGGAAGGTATGAAAGACGGCACCAAAACAATTCAAATAACAGTGGATGAAACCAAGAAGATTGACAAGGGCACTGACATCCTCATTAGCAAGATTAGTAGAAGTGATTACGAAAAAGCCATTGCTATTTTTTTGGAATTAAGAACTGGCTATCAAAAACTGGCTTCTTCCAAAAAGGGTGATATTTATCGCAGTGAAAATGGTTCAGAGATCTTTCTTAACGGGATGAAAATCGGAACCGATGAAAACTTCTTGTTTTCTTATGACATTAAAGAACCCAATAAGAAATTGCAAAAATCCTTAAACCGGGAGCGTAAAACACTTTCCCGTGATAGCTACCGTGACAATATCATTTCCATTTTGAAGTCCAGCATTAATAAAAATACCTAAACTTTAATTAACCAATTAATTGACAACCGTGATCAGTTTGAAACTGGTGAATGGAGTTTTATTGATGTCAAAAAGTTAGTAATGCAAAACACTAACCGAAAGATTTTGTGAACATCCAATGAAAGCAGTGACATTGCAGCACCTGCTTATCAAACATGAGCACAAGAGGAAGGTTATGAAATTATTTCGATTGGTTCATCACAATATCAAAGTATGGAAAATGATGCCGAATTTAAAAGCTATACCCTCAACGATTTTGGTGATAGATTTGTCAATGAATTCCAAACGGAAGAGGTGCCATTCCACAAATTAACTGAGATCGAAAAAGACAACTGGAATTGGGTAATGGCTAAGGTCAAAGAACTTACCAGGGTTTGAAGTAATTGGAAAAATTTATACAAACACTATGAATTTTCGATCATTAAAAAACATCCTAATGCTGAAGGATTACATAGCAATGGAAGAATTCAAATAGTTAGAAAAATTCTTAATGAACGCTCTCACTTGTTCAACACTATCATGCACGAAATTTGTCATGCAACTAGCTTTAGTCCTGATGTATCACAAAGATTTGAACAAGGATTAACCAGTGCATTTTATCCAGTTATGAAATTGAAACCAGAATAAAATGGTTTTCTTTTCTATAAATGTCTAGCTAAAACTTTTTTGCCAAACGTTTTTGACCTCAAGTAGAAGCTTGTTCAAAAAATCATCATGGCCTTATTAAAGTTTTTCAACCAAAGTATTGCCCTTCTTTTAATTCAATTGGGCAAATTGTTAAAATTCCCAGATTATGTTTTATCTCGATTTAAACATTCAAAATATTCTTACCAATTGGGAGATAGCTGATGCTGTTCGCGAACTGATCGCTAACGCCATTGATGAGCACATTTAACCGAAACCCAATTCCCTAAAATTGAATTCAACGAAAACAATTCAAGCTCAACATTAATAATTCAAGACTTTGGTCGCGGCATTAAAATCGAACACTTTATCCAAAACGAAAGTAAGGAAAAGGTTCAAAGTGAAAAAACTATTGGTAAGTTTGGCACTGGTTTAAAGGATGCAATTGCTGTTTTATTTCGCACTAATATCAAAGTGAGTTTTACAAATCAGCAAGGTACTTTTATTCCTGTAGAACGGAGTAAAACTGAACTGGAAGGTAGCATCAAAACAATTCAAATGGAAGTTGATCCAGCTGAACAAATTGAAAAAGCGGGTACCTTAATCACAATTGAAGGATTGAAAAAAGATGACTATGACAAAGCTATTGTTAATTTCTTAGCTCTACGGGAAGATTTGCAATTATTAGCTGCTTCACCCAAAGGCGATGTTTACCGTAATACATCAGGTAACGGCGCAGAAATTTTTCTCAACGGGATGAAAATTGCAACCGATGAAGACTTTCTCTTTTCCTATCACATTAAAGAACCTAACAAAAAGTTACAGCGTTCCCTTAACCGGGAAAATAAAAATTTACCAAGAGATTGTTATCGTGAAAACATCATCACCATTCTTAAGTCCAACATTAACAATAGAACACAAACACTTATTGATGAATTAATAGATTCACGTGACCAATATGACAATGGCGAGTGGAGTTTTATTGATGTCAAAAAACTAATTGGTTTAAACACAAACAGAAATATTTTGTGAGCCGATAGCAGTAGTAAAAACATAGAAAAACTAATTTATTCACTTTATGGAATGGATACCAAAAAATATGAAATTTTGGCGTTAAATTCCTTGCAATACAGAAGCATGGAAAACGATGATCGTTTAAAAAAGCAAACACTGATGCATGTAAGTGAAAAGTTAAAACAACAAAGAATTGAAGAAGAGGCTGAGAAAATCAAAGTTAAAGAACAAAAGCCACGAAAACGATTTGAGGAAGAAGATTTACCAATAGAAGATTTAAATCCAATTGAAAGAGAAGGTTGGGATTGAGCGATGGAAAAGGCCAGAGAATTATGTGGTTTTATAAGAGGATGGGAAAAACTTTATGAAGAATATCAGTTTGTTCTAATGGAAAAGAATCACAAATATGTCGGGCTTTGTTACACCGATCAAAAAATTATCAAACTCTCAAGAGGAATATTGAAAGATGAATACAGTTTATTGAATACCTTAGTGCATGAAATTTGTCATGCAACAACTAATGGTCGAGACGGAACGAAGAAGTTTGAAAGGGGTCTAACTGATGCTTTTCATCCATTATTTAAACTAGGGCAAAGTAAATAATTAGTCCGTGTTTTACATTGTCGTTGAATTTCCGCAACCGCTTTCCATTAAAATAATTTAACGCCTGAATAAAGTGATGAGTCCAGAAAAATACCTGAACTTCTTTAAAGAAACCGCTGATAAAAAGTTCCAATGACTCAAAGAAGAATTGAGCAAAATCCGTACTGGTAGACCTAATCCAAAACTGTTTGATAATCTCCTTGTGGAAAGCTATGGTGACAGAATGCCAATGGTAGCATTAGCACAAATTGCGGTAAATCCACCGCGGGAAATTGTGATCAAGCCGTTTGACGTTAAAAACAATATTAATGCCATCTACAGCGAAATTCAACGTGCTAACTTAGGTGTACAACCAGTGATTGACGGAGATAAAATTCGCATTAACTTTCCCCCAATGACCCAAGAAAGTCGTTTGGAAAGCATTAAGCAAGCTAAAAAAGTAGTGGAACAAATCCACCAAGAGTTGCGCAGTGTGCGACGTGATACCCTTCAAATGATTAAGAAGGATGATCATAAAGACGAAGACTTTGAAGAATTCTTAAAAGAAGAAGTAGAAAAAGTTAACAAGCAGTATATTGCACAGTTAGAAACTATTCAAAAACAAAAAGAAAAAGAACTATTAGTGGTCTAATGGATCTGAAAGATAATTCCTTCGCTAAAAAACGATCGACAGTGTTCGTTGTTTTGCTCATTGTCTTTTGTTTTTTTTTAATGTTTTCAGCCTTTGCTGACGGCTTTAACTTTTGATCGCCTTGGTCAACTGATTTCAATTCACGCGTAATTCACATTAAAAGTGACGGTAATATAGAAACCACTAGTATTGTCAGCCATGAGTTGCATCCTGACTTTAAGGCAGTCCGTTTTGCTTTTGGCTTAGTGATAGTTTTATTTATTAGCGTTATTGCCGTTTTAATGAATTGGGAGTTGTCTAACAGTATCTTTAAAAACTATGAAAAGCTCAACTTATCCTTATCACTACTTAGTGGAATTATGGTTTCAGGTGGGATGATTCCAACCTTTTTCGTAATCTATTTCCGTGAATGAAATGCGACCGTTAACTGAATTTGAACTGCCTCGTTTGCTGGCATGATTGTCTTTTTATGGGCAGTGTACATGATTAGCACTTCCTTCATAAAGATCCGACCATCATTGCAAGTGATTTATAGCCTAGGTGCAGTGATCTGTTTCATCGCTTGCATAGGTACAATTTACTTTTCCGTAATTAGGGGATGGACTACCATTTTTCTCTTAATTGCTATTGGCGTTTGCACTGATACCTTTGCTTATCTGTTTGGTAAACGTTTTGGAAAAAATCCCCTCATTAAAATTTCTCCCTCAAAAACTTGGGAAGGTGCTTTCTTTGGGGTTACTGGTACAGTTTTAACCATTTCTATTATTTGTGTTTTGTACTCGATCCCTAACTATGTAAGACAACCTAGCATAAAGGATGCAAGTAAGACAGCACTACAAACACCACAAAACTATGATGTGCATAACCTTATTACCAATGTCTTTCTCATCTCCTTCATTTCCGGTGGTAGTACCTTTTACATCTACTGGTGAGTTAGCACATTAGCACTGATATTTACTGCTTCCATTTTTGCCATAGGTGGTGATTTATTCTTTAGTTACATTAAAAGACTCACAAAAATTAAGGATTTTTCGAAGTTATTGGGTAAACACGGTGGTATCTTAGACCGGTTTGATTCCAGCTCTTTTTTAATTAGTTTTTTCTTTATTTACCACGTAATTGCGGGAATCTCTTCTAACCAAAGATTGCTTATGGAACCAAACACTTATTTCAGTGCTGTAAGTTAGTTTTTAGAATTTAAAGGAAATGACTCCTAAATTAAAGCTTAACACTAACAGTAATTGAACTAAGAAAACCCTTGGCAGCTTATTTGAACTCAAGAAGGGTGAAATGCTCGAAAAAGAGCTATTGGCGCCCGATGGTAAATACGAATACTTTAACGGTGGTATTAAAGCCTCTGGACGCACCAATGAGTTCAATACCTTTAAGAACACCATTAGCATTATCATAGGCGGTTCTTGTGGTTATGTCAGATTAGCCGATAAGGATTACTTCTGTGGCCAGAGTAGCTGTACCCTAACTGTTTTAGATCCCCTGGAAATTGACCTCAAGTTTGCTTATTATGCACTTAAATCACAAGAGGAAAAAATTACCAGTTTAGCTAGCGGAACTACCATTAAAAACATTCGCCTAAGTGATTTAAAGGATTTACCTATTCCGCTTGTTAAAAGCATTCAGGATCAACGAACTATTGCACATGCTTTAAGTGTGTTTGACCTTCGCATCGAACACCTCAATGAATTAATTGAAGTAAACCGTAAGCTCAGAGACGAGTATGCCCACAAATTATTTACCTTAGATCCAGACTTCTTAACACACTGAAACCTACATGAACTCCATGAACAAATGGGTGAAATTAGTTTAGGTGAAGTGTTTCACTTAAAGAGTGGTAAATACTTAAAGGCTGATGAGCGCTTTGAGGATGGCAAATTCCCTTATTATGGTGCTGGTATTGAAAGCACTAGCTTTGTCAATGAACCCAACACCAAAGGTGATACTCTTTCAATGATTGCTAACGGTTACTCCATCGGAAACATTCGCTACCATACTATTCCTTGGTTTAATGGTACTGGTGGTATTGCCATGGAAGCATTAAAACCTAATAAAACCTATGTGCCGTTCTTTTACTGTGCTCTAAAATACATGCAAAAGGATCTAAAGGAACGCTTTAAACGTGATGAATCACCGTTTATTTCCTTAAAACTAGCCGGTGAAATTAAGGTTCCTTTCGTTAAATCGTTTGCACTCCAAAGAAAAGCCGGTAAGATTATTTACTTGTTAGATAAAACTCTTGAAGAATGCAAAGAAGAAGCTAAGTCTTTAATCAGTATTCGCGATAACTTATTGGGCAAATTGTTCCCAACACTATCTTAAAGCAAAGCAAATAAGGTGTAAGGGAAAAGGTTAACACCTTCGTTATCAAAGCTCTGGTCTTTGAGACTGCTGGTTAAGCTATTCTCCAATAACTTCTTCATTAGATCACTCGTACTAGAAACTTGAATTCTTAACTTTGTGTTAAGTTTGATGTTACTGGTAAAACGGTCAAATGAAGCGCGGCCAAAGATGCCTTGAGTAGCTGGACTATATGTAAAAGTGGCTTCTAGAGCTTCATTTTGACCCATACTCATGGTTAAAGAATTTTGAGCATCTTTTTTCTTAGTTTCATCATCCTTAAAAATATTCTCTATATGGCTCATCGCCTTGGTTGGATCAATTTTTAAATCTGCCTTAATCTTAACCGTAGCCTCAAATTGCTTATTCGATTCAACTTTGTAGGTAAACTCCTTCACTTCTTGCTTGCTAACAGTGATGGCTTGTTCAGCTAATAATTGAATCTCAGTTGTTGAACGTTCACTTAACTTTAAACCCTTAAGCGTAGCATTGGGTTGAACACTGTTTTGGTTACTACCGTTGCCGAAGGATTTAGCAAAGCGGCCTACTTCAGTTTGCCAACGTTCTAATATTCTCTGTGAAAGCAAAGTGTACCTTCAACCTTCAAACAAGAGGTTTAAGGTACCTTTAGGATTTAAGCCGTAACTTTCGCGTAAAGTTGTAATTAAGGTACGTTTATTTTTCGTTAGTTCTGTGTTGCGATCTGCTAGGTTTTGCAGTTCTGATAAAGATTTAAGTTCAACGCTGGCAACTAGAGCAGAACAACTGCTTAAAATAAGCGCTAAACTAGGGATACTAGCTCAAATTCACTTCCTTAATTTAAGCGCCATACTCCCTAACATGCAGTAATTAAACAATTGTAAAAATTACCGCAAATACTGTAAAAGGACTAAAAGAATTAAAGCAGAGCGTAGTAAAAAACGGTAATAAATTAAATAACTTACCTGTTAATTGACGACTTTTCATCTTTTTGACCTGTTCGTTGCTTTCTAATAATTCATTCAGCTTTGTGTGAGAAGTTGTTACATCAATCTTGACATTAGCGGGAAAGTTAATCGAACTAGTTGGTACATCAAAGGATTTATCAGAGAAGTTTCTAGCACCTCCAGTTGGTGGGGTATAGGTAAAAATAAAGTTACCAGTGAATTTGGCTTTTTCACCAGTGTATTCACTTTCATCAATACTGTTTTTACCATTGCTACCTTTACCTCCCGAACTATTACCCTCTAAGAATGATTTAATCAATGCTTTGCTTGATTCATCAATCTTTCCATCCATTTTGATTTGAATATTTACCTGAAACTTTTTTGGACTGTTTCAACTGTAAGAAAAACCACTAACTGTTTGCTTTTCAACTTTAAATACTTTAGCGATTTCTGAACTAATACCAGGAAAAGTGTCTGCTAAAAATAAACTTTTTTCACTAATGTTAGGGGTAATATCGTCCTTCTTGCTAGTAGAACCAAAAGCGCTAGCAAAGCGTGAAGCATCTAAATTTCTTTCTAAAAGCTTTTGATCCATTAAGGTGTACTTTCAACCTGCTAAAAGAGCGTTAACTGTTTTGTTAGGATCAGAGTTATAGCCATCTTTCAGAATGTTGATAACGTTCTTTTTGTTCAATTCACCTTTATTTGCTTCAAAGTATTTTTGCGCGCTACTTAAACTCGATATAACCTGGCTAGTTGCAGAGGAACAAGCGCTCAGCAATAAACTAGATCCTAATAAAGGAATTAATAACAGTTTAAGTTTCATTAGAGAATGGAATAAAGCAAGTAAGGTAAAAGATCAATTGATTGGTCTTGGAAAGTTTGACTGGACAAATTATTAGTAAAGGTAGTACTTTGAAGAATCTTTTTCATGATTTCGTCTTTACCAACCATATCTATTTTTAAGGTGGTATTGAAATTAATTTTCTTCATTAAAGTATCGAAGTTCTTTCTAGAGAAAAGATTTGATTCAGGTACAGTATAGGTAAAAATAAGATCTGCAGAAAGAGTGCCACTTTCTTGTTTCAAATCACCATCATTTAAACCAATTTGTTGTAGATAAGATTGAACTTGACTTTTTTGGTATTTTCCTTGAGCATTAAGTTTTACTTGAACTTTAAAACTAGTTCGACTTTTGTATTGGATATTAAATGCTTCTACTTTTTGCTCATTTAGCGAAATAACATTGTTGATAATATTAGCAGTGCTTGGTTCAAATCGTTTATCAAGTCTTAAACCTTTAACACTAGCATTAGGCTCAACATCTTCATTACTTTTTCCAGAGCCAAAAGCTTTGACAAATCTAGAAGGGTCTTTGCTCTCCAAAATTTTGGAATCTAATAAGGTAAATTTCCAAGCGTCTAATAAAACTTTGGTAGTTCCTTCAGGATTATTTTCAAATGCTTTTTGCAGTGACTCCACAAAAGCTTTCTTGTCTTGGGTTAGACTGGTATTTTGATTAACGCCTTCCCTCAATTGATTCAAACTTGTTAAATTAGTTTGAAAATCAGAATTAGAACAAGCAGTTAAAGCTGTTCCGAACAACATGAATAAAGCACCTAATGAAGCTTTGGGTAATAGTCTTTTCTTATTCATCTTTAAAGTAGAGCATAGTAAAAGAAGGGTAGCAAGTCAATATTTCTTCCATTAAATGTACGACTTTTCATTCGCTTAACTTGATCGTTTTTCTCAAGCAGTTCATTTAGTTTATTATGCGAAGTGTTCACATCAATCTTTACATCAGCAGGGAAATTGATCATATTGGTGATAACATCAAATGATTTATCGGTTAAAGATCTAGTACCTCCAGTTGGTGGAGTATAGGAAAAGATAAAGTCTGCAGTGAATTTAGCTTTATCTCCAGTATATTCACTTTCTTCTACGCTATTACTTCCATTTGAATCTTTGTTACTCAAAAAAGACTTAATTGTTTCCTTACTCTTTTCATTAATCATCCCTTCCATCTTTATGTGGATGTTTACTTTAAAACTTTTAGGGCTGGTTCAGCTGTAAGAAAAACCACTAACAATTTGTTTTGTACATTAAACACTTCAGCAATTTGACTTCCTAAACCAGTAAAAGTTTCATTTAAAAATAAACCTTTCTCGCTAATATTCGGAGTAACGTCATCTTTATTTTTATTCGGGCCAAAAGTATTTTTAAAACGAGATGGATCTAAATTATTTTCCAACAATTTTTGATCCATTAAGGTGTACTTCCAGCCTGCTAGTAAGGCGTTAACTGTTTTGTTAGGATCAGAGTTATAGCCATCTTTCAGAATGTTGATAACGTTATTTTTATTCAACTCACCTTTATTTGCTTCAAAGTATTTTTGAGCGCTACTTAAACTCGATATAACCTGGCTGGTAGTGGAAGAACATGCACTTAAAATAATGCCGGAAGCTAAAAGAGGAATTAAATAAAGTTTAAGTTTCATTAGAGTATGGAGTAAAGCACGTAAGGCAAGAGATTTATCGATTGGTCTTGAAATTTTTGATCAGCTAGTTGCTTTACAAAACTACTTTGTTGCAATAATTTAGTCATCACACTATCTTTGCCTATAATTTGTATTCGTAAATTGGTATTAAAGTTTATGGCTCTTGTTAGTTTGTCAAATTTACTTGCAGAAAAGAAACTGGCAGCAGGTGGCATATAGGTGTAAATTATTTCAGCTTCTAATGTACCTGTATCATTTAGATCACCATCTGATAAACCTACTTGACTTAAATAAGTTTTTACAGTATCCTTTTTGTAATTACCTTTAGCATTAATTTTTACTTGAACTCTAAAGTTTTTAGGATCTTTGTATTGAATGCTAAAAGCTTCTACTTTTTGTTTGTCCAGTTTAATTACGTTATTAATTATATTGGCAGTGTCTTGTGTGTATCTTTCCACCAAGCGTAAACCCCTTACTCCAGCACTAGGCTCTACATCTTCAATGCTTCTGCCAGAACCAAAAGATTTACTAAATTTGGAAAAATCTTGTTTTTCTAAAATTTGAGCGTCTAAAAGCGTAAACTTTCAAGCGTCTAATAAAACCCTAGTAGTGCCTTCTGGATTATTTTCAAAAGCTGATTTTAAGGCTGTAACTAAATCAGCTTTATTTTGGGTTAAATTAGTATTTTTACTTGCACTGGTTCTTAATTGATTCAAGCTAGTAAGATTTGCTTCAAAACCAGAATTAGAACAAGCAGTTAAAGCTGTTCCAAACAACATAAATAAAGCGCCTAAGCTTGTCTTATTTCAAATTTTCTTTTTCATATTTTTAGAGTAAGGCATAAAAATAGAAGGGCAATAGATCAAATGATTTACCAGTAAACGAACGACTTTTAATTTTTTTAACATGTTCGTTGTTTTGCATTAGGATATTGAGTTTTTCATGAGTTGTGCTTGCATCAATTTGAAGATTAGCAGGGAAATTAATTGTTCCAGTGATTGGATCAAAGTTACTAAATGCAAATCTTCTTCCATCTGTAGGCGGAGTAAAAGTGAATATGAATGTGCCATCAAAGTTTGCATTTCCATTATTAAAATCATTTTCAGTAACATTGCCATTATTTCCAGAGCTCAAGAAAGATTTAATTAAAGAATTACTTTCTTCACCACTGCCAACAATGCCTTGCATCATAAAAGATATAACTACTTTAAAGTTAGTGGTGCTAGATCAGCTGTAAGAAAATCCAGTTACTTTTTGGCTTTGCACTCCAAGTACTTTAGCTATTTGTTGACTTACACCACTGTAAGTTTCATCTAAAAATAAACCTTTCTCACTAATACTTGGAATGACATCATCTTTGCCTTTGTTAGTACCAAATGCTTTTGTAAAGCGACTAGCATCTAGATTTCTTTCCAGTATTTTTTGATCCATTAAGGTGTACTTTCAACCAGCAAAAAGTGAATTAACTGTAGCTTTTGAATCAGTACTGTAACCTTCTTTTAAAAGATTAATTACATTCTTTTTGATTAGTTCACTTTTGTGAGCATCAAAGTATTTTTGCGCACTACTTAAACTAGAAATAACATTGGTGGCAGTTGATGAACAGGCACTTAAAGCAGTGCCGAACAATAAAAATAAAGCATCTAATGGAGTTCTGAATAATAGCCTTTTCTTATTCATCTTTAAAGTAGTGCGTAGTAAAAGAACGGTAACAAGTCAAAGGTTTTACCAGTAAACTTACGGCTTTTAATCTGTTTTACTTGTTCATTGGCTTCCATTAAAGTATTTAACTTTTGGTGAGTAGTGCTTACATCTATTTGTAAGTCTGCTGGAAAGTTAATCTTAGTTGTAAGAGGGTCAAAAGAAGTGGCACCAAATTTTCTACTTTGAGTAGGAGGTGTATAAGTAAAAGTAAAGAAACCATCGAAATTAGCAGAATCACCATTAAAGTCAGTTTCTTTTACTCCGCCATTTTGATTTGATCCATTGTTATCAGAACTTAAAAAAGATTTAATTAATTGTTGACTCTCTGAATCACTTCCAACTTTTCCCTTCATACTAATTTGAATTTGTACTTTAAAGTCAGAGTTACTGGATCACTTGTAATTAAATTGTTTAACAGTTTGACTTTGTACACCTAAGACTTTGGCAATTTGACTACTAAAGCCAGTGTAAGTTTCGTTTAAGTACAATCCCTTTTCACTAACACTAGGAGTTACATCGCCTTTATCGCGTCCGCTACCAAAAGCTTTGATAAAGCGACTATCAACTTCTTTAGAAAGTAATTGCTGGTCCATAAGTGTGTACTTTCAACCAGCTAGCAAAGCATGGACTGCCTTATTAGCATCAGATTCATAACCTTCCTTTAAGATTTTGATGACATTCTTTTTGTTGAGTTCACCTTGACTGGATTCAAAGTATTTTTGAGCACTACTTAAACTAGAAATTACTTGAGTAGCTGCTGAAGAACAAGCGCTTAACAACAAACTAGAACCTAAAAGAGAAATTAATAGAAATTTAAGTTTCAGTTTCATTAGAGGATGGAGTAAAGTACGTAAGGCAGTAAATTAACTGCTTGGTTTGGGTAATTAACACTGGCAAGATTATTAGTGAAGGCACTACTGTTAAGAATCTTTCTCATTACTTCGTCTTTACCAACCATTTCTAATTTCAGATTTGTATTGAAATTAATCGATTTAGTTAACTTGTCAAAATTACTCTTAGAAAACAAATTTGAAGCAGGTGGAGTGTAAGTGAAGATTAAATCAGCTTCTAAAGTGCCTTCTTCCTTATCGCCCAGTTCATTATCTTGAACACCAATTTGTGACTTATATTTCTCGACTTCACTCTTTTTGTAATTACCTTTTGCATTCAGCTTAATCTGAATAGTGAAATCACGACTACTTTTAAAGCGAATATTAAAAACCTCTACTTTTTGTTGATCCAATAAAATTGCATTTTTAATAAGACTAGCTGTGTTATCACCAAAACGTTCAACAAATCTTAAACCACGTACGCCAGCACTAGGTTCTATGTCTTCCTTACTTTTACCGCTACCAAATGCTTTAACAAATTTAACTGGATCTTGTTTTTCCAAAATCTTCGCATCATAAAGACTAAACTTTCAGGCATCTAGTAAAACCTTAGTGGTACCTTCTGAATTATTTTCAAAGGCAGTTTTTAAAGCATCAATTAAGGTATTTTTGTCCTGAGTTAAATTAATGTCTTTATTGGCTGAAGTTCTCAATTGGTTCAAACTAGTTAAGTTAGCTTGAAAGTCATAGTTAGAACAAGCAGTAAACGCTGTTCCTAATAAAAGAAACAAAGAACCAAAAGCGGTTTTAGATCAAATACTTTTTTTATTCATGCTTCGAAATAAGGTTTAAAAATTAGAGAAGGGCGTAGTAAAAGAAAGGCAAAAGATTAAATGATTTGCCTTTAAAAGAGCGGTTCTTAATCATTCCTACCTGAGTATTAGTTGTCATTAAAGTGTTTAACTTTTCATGACTAGTACTTACCTCAATTTGGAGATCAGCAGGAAAATTGATGGTTCCAGTAATTGGATCAAAGTTGTTGGACGCAAGTCTTCTGCCATCGGAAGGTTGAGTGTAAGTAAAGATAAAATTGCCATCAAAGTTAGCTTGGTCACCTTCAAAATCTTTTTCAGTTACACCACCATTTTGATTATTGCTTCCACTGCCTTTGCTATCAGTACCTAAAAAAGACTTAATTAAAGTTTGACTTGTGCCATCACTACCAACTTTTCCTTTCATTTTGATTAAGATTTTGACCTCAAACTTGGTTTTGCTAGTTCAGCTATAAGAAAAACCAGTTACCTTTTGGCTTTGGACACCAAGAACTTGAGCGATTTGACTACTTACACCGGGATAAGTTTCTGCAAGATATAAACCTTTTTCACTGATGTTCGGGTTAACATCATCTTTAGTGTTAGAACCAAAAGCTTTAGAAAAACGGGAAGGATCCATTGGGTTCTCCAATAACTTTTGATCCAGCAAGCTGTATTTTCAGCCAGCTAATAAAGAATTAACTGTTGACTTGGGATCAGAATTATAACTATCCTTCAAAATGGTTACTAGATTACGTTTATTAAGTTCTTTTTTGTTGGCACTAAAGTACTTCTGGGCACTACTAAAACTGGAAATTACTTCTGTGGTGGCAGAGGAGCAGGCACTTAAAGCTGTGCCAAACAGGAAAAACAAAGAACCTAGACTGGTCTTTGACAAAATCGATTTTTTGTTCATTTTTAAAAAAGTAAATTTTTTAGTTGATTTATTGATTAGTCAGATGGTTTAAAAATACTTGTTTACAAACAATGAGATAAGAGGAGATTTATCCTCTTATTTAGTATGAGTTAACGCAATTCAAAGAAAAGCGAGCGGTATTTAAAAAAACAGAAGTAATCACTAAGTTTGGAATTAATTACCTCCTTTTCAATGACAACTACAATCTCTAACTTAGGCGTTTCTTCTATTACTTCTGGTTGATTGTCTTCTTCTATGCTTGGTTGTTCAATTAAAAGGCCAATAAAATTATGAACTTGGCGTTCAATAAAGAAAACAAAGTTGTTTAAAGAACTAAAATCGTTTTTAATACTTCTTCAAGCTTTAATTAACAGATTAAAGGTATAGTAGTTATTTAATGAGTGGGTGACACGAAAATTAAATAACTTAAATAATGTTTTCGAAAGAAAAACAAGACCGTTAATTAAAAACAAGGAGACTAGTAAGATAGCTAGTACATTTGCTTTAAATTGGCTTTTCATGAAGAAGAAGGTTGGTTAAAATTGAAAGCTTTTTGAACTAAACAAACCCAACAGCTTGACTGAAACTAAATTAGTTCTCGTCTAATTGACCATAATTTTAAAACTTTTAATTGTTGCTTATTCCGCTAATAAGCTCGTTTAACTAGCTCGATTCAAATAAGTAAGATGGCAAAATTAACTAAATTGGCATTAATCAATATTCAACTGTTATTCTAAACTGACTTTTAACTAAAAAAGTAGATCGATTAAGTTAACTGATTCTTAATAAAAAAGCTTTCATTTTTCAACCAAAAATTATTTTTCAATTGAATGTCTTAAAAAACGAAAAAATCTTATTTCTTGTTCTGTTCGATCATCGAAATGATTTTCAAACTCTTCTCTGTCCGCATTACTTTGACCAATATATTCCCAACTTGATGTTCCATAAAGAATTATTTCTCCGTGTTCCAAATTAAAACTATTGTTGCATTTGGTAGTTTTATCGGGATCTTTTCATAAGAAGTTTTTAAAGATTTAACTAGATTTGTATGGCCTGTTTCTGTTTCAATTTCTTCAGACCAATTTAAATCTTCGTTAGTATTGTTTTAATCAAACTGTATATCTCTGATTTGACATTGGAACAAGCACTTAAAGCTGTATCGCACAAAATAAATAAAGCTCCTAGACTTGTTTTAGATAAAACTTTACTCTTACACATAACGAAGATAGATGAATTAAATGTTGATCCATTTTGCTTTTTTTGGAAAAGACGTGCTTGAAAACTTCAAAATCACTTAATTTTTAGGTTTCCTGGTTTGTAATTTTGAAGTAAAAAGCTTTTAAAAATTTTTAAAAAAGTTTTTTATTTAACTAAAAAGTTTTGAGGAATTAAGTAAATCTTAATAAGTTATCTAATTACGTAAACTAAATAACTAAAAAGGTTGATTTTGTAGTCATCAATTTGAAAAGCTCTTGCTCTACCAAAAACACTTAATTCAAAAAGTTTTTTGCTAACTTCGTCTTTTCCTTCTAATCTCCAGCTAAATGAAACATAAAAGTCGATTGCTTCTGAATCAGGTTTAAAGTTTTCTGCTGCAAATGGATGGTTGGGAGAAGCTTTAAAAGTAAAAAAGAGATCAAAATAAAGCTTTTCACCTTTTTTTGGAGCGAAACCTATTGAAAAATCATTGCCATACCATGTAAAGGTATAGTTCAATTTGGCAACAACTGAAAAATCACTAAGGCTTTGGTAATTCAAATTGAAATACTCAACCTTTTGTTCGATACTAAAGAGACTAGTGCTTAAGGAAATTTTCAATTAGTTCAATTAGTTTCAGAACTCTCTATTTGACCGTTTTCTACACTTTGCTTCCAGGCATTCAAGAGTACACTAGCTGTTTTGCTAGGGTTAGTTTCGTAAGACTTCTTTAAAGAACTTATTAAATTCTTCTTTCCCTCAGAAGTACTAACTGATTCAGACAAATCAAGGTCTTGATCGGTGGTTTCCTTAATAAGACTTTTCAAATTAGCTCCAACATTGACGCTAGAACAAGCGCTAAATAATAAAGCTGAAAATAATAACGGAGTTGATAGATATTTAAATTTCATTAGAGGACTGAATAAACCAGATATAGCAGTAAATTTACTTTGTAATCAAAGTAAATTTTATCTACCACTTTTCCAATAGAACCATTATCTAAAAGTTTTTTGGTTATTTCATCCTTAGGAAAAACTTCTAGTCTCATTGTTGTTTAAAAATCAATGGAATATCACAAATTCCCTAATTTATTATCAGAGTTTTTAACAATACTTGTCTCTAGAATTGGTTTGTTTTTAAATGGTTTGTATGTGTAAATAAGATCAAAATTAAGCAAGTAGTTGCCAAAGCCCGACATTATTAACCCTCCTTGATTTGGTTTTTGATTAATATCAGCATTAAAAGTTGTGTTCATTTTAACAACAACCTTAAAATCATTTTTATTGTTTGCCTGAATGTCAAACAATTCAACTTTTTCGCTAATTGTTATTGATTTCTTGTGGAATTAAATGAATGGATTGATAAAAAAGGTTAAACATTACTTCTACCTTCTTCTCTCATAAGACTATTAAATCATTTGGAATACCAGTACCACAGGTGCTAAACAATAAAACTGCTGATAAAAATGGAACTGAAAGGTACTTAAATTTAATTCCTTAATTCTGCTTTTTTTGCAAAAAGACCTTTTGACACCTTAAGCAAAGAATAAAAGTTTTATTCTTAAGATAAGGACTAAACCAAAAATGAGAAAGAAATTAGCAAAAGTAAAAGTTCACATTCAGTCTTTGGATTCATTGCTTTCATCAATGACGATGCCGATTATTGGCATCTTTATTGCTTGGGGACTTTTAGCTTCTTTTTTTATTCCATCTGGTTGAACACCTGATAAAAACCTAGCTTTAATGGTGGAATAGGAATCCAATATGTGATTCCGACCATAATTGTTTTTTTGGTGGTAAAAAGATTTATGAAATACGTGGTGGTGTGATTGCTGTCATTATTGCCATTGCTGTAATAGCTGCTGGTCAAACCGAAGCATTTACAAAAATTGTTGGTCAAAAATCAGTAATGTTTTTAGGTGTGATGATATTCGGCCCAATTGCAGCGCTAATCTTAAAACACACTGAAAAGTTTTGAATTCACCGTATTAAAAGTGGCTTTGAAATGCTGGTAAATAATTTTTACCTTGGCTTTTTAGGTTTCGCCTTAATATTTCCTAGTTTTTACCTATCAATTTACTTAATTGGTTACATTCAATTAGGTTTAAAACTGCTTGTTGAAATAATGCAACAGTACAAACTTTATCCAATAGCTGCCATTGTGATAGAGCCGGCCAAAGTTTTATTCCTAAATAATGCCATTAACCATGGCGTGCTAACTCCATTAGGCTTACAACAAGTAAGAGACAGTGGTAAATCAATTCTTTTCTTATTGGAATCAAATCCTGGTCCAGGTTTGGGTCTCCTTGTTGCTTTCTTAATCTTTTTCTTTAAACGTGATAAAAAGCTTTCTAGTAATGCGGCATCTTCTAGTCCTATTCACCTCTTTGGTGGTATCCATGAAGTTTACTTTCCGTTTGTGCTATTAAAACCTGTGTTAATTCTGGCCACAATTGCTGTTGGTGTTGTGGGAAACGGCATTTTGCAAATATTTAATGCTGGGACAATTGCACCAGTGTCACCTGGTTCTGTTATTGCTGGTTTTCTCCAAATTAATAAAACACCTTTAGATGTAGCTGGTTATGCTTTGGCACTCGTTTTATCAGCTGTAACTTCGTTGTTAATTTCGCTTTTATTACTTTCTTTAACACGTAAAAAACAATTGAAAACACTGCAAGAAGCGCAAGCACAAGTTGCAGAAATGAAGCAAACGCCTGCAAAAAAACCAAGACAAAAAGATACTCCAGCAATTGCTACAAAGATAGACTTTTCCCAAGTTACCTTTGTTTGCGATGCAGGTATGGGTTCTTCTACTATGGGAGCAGCTATATTTAGAAAAGAACTAAAAAATCAAAATATTGAAGACATTACAGTGATTAATAAAGCAATAGTTGATTTAAAAGATGAAAAGGTAATTATTACCATTTCCCAACTTTATGATCGCGTAAAAGCGAAGCGTGCAGACGCTACGATTTATACCATCAATCAGTTCTTAGATAAACAAGGCTATTTAACTATTATTGAAAAGATTAAAAATGAAAAGAATTAATGTATTGCACTTTGGCGCTGGTAATATAGGTAGAGGTGTTATATTACCGATTTACCAACAAAATGACTTTTCCATTGATTTAGTGGAATTGAATCAAAATACAGTTAATGAGTTACAAAAGCAAAAACAATACCAAGTTCATTACCTTGATTGTGATCAAAGTCAGTTAGTTAATGACTTTAATACTTGAAACTTAAAGGATGAAGCGAAGATTATTGAACTAATGGAAAGAGCTGATGTTATTTCCACTTCAATAGGTGCTAAAAACTTAGCTAGTTTAAAAACTTTGTTTGACAAAGCTAAGTTCCATAAAAGGGCTATAGTGCTTTGCTTTGAAAACGGCTTTCGGATTAGTTCTAACTTTAAAAACATTTTGCAATTAAATAACACACAAGTAAATTTTGTGGATGTGGTAATCGATACAATTGCACCTAACTTCGAAAAGAAAGCGAATTTCTTGGATATTTATTGCGAGAAGTATTCAGAAATTTATGCTGAAACTTTCCCGCTAGAAATTAAGGGAGTAAATCAAAAGAATAGTTTAGATAGATTCATTATTAAAAAACTTTTATTAGTCAATGCACTGCACAGTGTTATTGGACTTTTAGGCTTTCAACAAAAGCTTAAGTATGTACATGAAACATTGCAAGTTAAAAGTAATTTGACTTTTGTAGAAAAATTAGCCCAACAAATTATTGATGCCCTTTGTGCTGAATATCCTGAATTTAATAAAAATAATTTGTTAAGTTATGGCAAAAATAACTTAGTCCGTTTTGCTAATCCCAAGATTCAAGATTTAAACACACGCTTAATTAGGGAACCATTACGTAAACTTAACCAAAATGAACGCTTTTATGCCATTTATAAACTCTTTAAAAAGAATAAGATTGCACTAAATAATATTTTGCAAGTTTATCTGATGGTTTTAAAAACTAATATAACAGATGATACCGAAAGCCAACAAATTGCTAAATTAATAAATGAAAAAGCTTGAACAGAGTTAGCGAAATTATCCAGTTTAGAAGAGTCAGAATGAAACCTAATTAAACAAGAATTAAGCCGTGAAATTACTAAAAAATAACATTTACATTAACGTTTATTTAAAAAACAAACAAGAAATTTTTGAATTTGTTTTTAAAAAGTTTAAAGAAGATGGTGCTGTACTAGATAGTTTTTTACCAGCAATTGTGGAACGCGATAAAGCAGCTAGTGTAGCAATTGGTAATTACCTCTTTTTACCCCACCCAGTTTATGACGAAATAGCAAACATTCAAAAAGAAAAAATGGTGTTTATTGGTTTAAAAGATGTAATTAACATTGATGGTCAACCAATTAAATTTATCTGTGGTTTAGCCTTAAAAGGTGAGCACCAAATGGATGCGTTGCAATCTTTAGCAATAGCTTTTTCAGACCCTGAAGAAGTGGAAAAGTTAGTTAAAGATAAGGATTTAACACAAGATAAGGTACTTGAATTTTTAGCAAAACACAACTAAGCAGTCACAATATTACTTAATTAAAAATAGTAAGTAACTAAATAAATTAACTTGCTTTTCGTCAGTTTTGTAACTAATATAGTCATTTAAAAAATTTTATCCAGATTTTTTTGGCTAAGTTCATCTTGTCCTTTTAATGATCAGCTAAGTGAAACTTTAAGCTAAATTCATTCTGTTACTATTTTTTCAGCTTTTAATTCTTCATTTTTAACGATGAAGTTTTCTGCTGTAAATTGTTTTTTTGATTAGGTTTAATAGTAAGGGAAAGATCGAAAGAATGCTCTTATCACCATTCTTGGCAGTGAAGCCATTATTGGAATACTGTCCATGCCAATTAAAGGTATAGATCAACTTAGCACTAATTGAAAAATTTTAATACTTCGATATTCAATGTCGAAACGCTCTACCTTTCGCTCCATTTTTAAAAGGATCTTTTTCTGTTGGATTAGTTACTTGGTCAAAATCAAGATCCGCAATGCCTAATTTGCCCTCTTCTGAGCTTTGTTTTCATGCGTCTAACAATAGCTTTGTTGTATCTTTTGGATTAACTTCATAAGATTTCTTTAAAGAATTTATGAGGTTTTGCCTACCTTCCGGAATTTTAATTGCTTTAGACAAATCAAAATCTTTGCCAGTTGTTTCTTTAAGAAGATTCCTAAGATTGGCTTGGATCCATGTGCAAGCACTTAATATTGTTCCACATAACAAAAACAAAGAACCGAAGGAAATCCTTGACAAAAGTTTCTTTTTACGCATTAACATAAAAAATAATGCATGATTTTGCTTTTTTGGAAAAGCCTTTTTAAGAAGAGGAAATTAATTGAAATTTCTGTTTAAACGTTATTTAAAAAATGAACTTTTTAAATTAGAAAAGCTATCTTTGTTGGTATTTTTATTCATCTAAAAAATACTCAAAAACTTTTTGCAAGTTTTGTTAGCACTTTTTCTCTACTCATGCAATTCATTAGTTAGAAGGGTGACAAGTGGTTTTTTTAGCTTGATCAGTGTGTTGCCTAACTTTATTTACTTAAATTACCAATGAGCAACAATAATAAATATTTCACTATAACTAAAAAACAGTACAAAAAAATGCGCCGCAATAAAATTGAACTTCTTTTTAATGTGAAAGTCCTAAAAAAGAAAAACGGTCGACAAAAGTTCAAAATACTGCATGAAGTTGAGAATAAGCCAAAAATACCAATTAAAATAATTGAAGATCAACCGGAGTCGCCAAAGCCACTTAAACCCCCTAAACCGCCTAAACCGCCTAAAGGTCCTGACAATCCTGAGGAGCCGGATTCTCCAGAAGAACCCAAAGAAACAGATCAACCAGGTGGTCCCGATAATCCTAATGCAGGAAATAAAAAGATGCCAACACCTGAAGAATACGTAACTCGTAAAGAATTCAACGAATTCAAAGATTCAAACAATCAACGACTTACAAAGATTGAAAATAAGGTTGATAAGTTAGAAGTCAAAGTCGACAAATTAGCTGAAATTGTTCAAACTCAGGGCGAAGAAATCAAAGAACTGAAAGTGGAGCAAAAAGCCCAGAGTGAAACCCTACAGCTAATTTTGAAAACACTGCAAAAGATGAACGATCGTCTGGACAGAATGGAAACTCGCTTAGACAAGATCGATTCGCCCAAATAGCTTTAGGCTAAAAAGAAGATACCAAGGCTAAAACGCTGTTGGCATTTTTATTCATCTAAAACTGCCCAAAAAAATTTTTTGCAATTTTGTTGGCACTTTTTAATTGCTATTTCAATTTATTTAGTGGAGGAGCAAAATGCAATTTTTTTGATTTTCTAGAATCCTCTAGAAAGGAGGTGTTAATATGAAATATAAAAAGATAGGTGATATTAAATCTAAAGGATTTTTCGAATCTAAAGAAGAATATAAGGAACTTTTAAAAAAATGTGAACTAATTTCTTTTGAAGCTTTTTCTGAAAAATTACCTAATGGTGATTATGAAGTAACTAAGAAACTTAAGCTTGTAGAAAAAGGTCCAAATTGAAAAAGAAAAACATACAAACAATCTTTAGAGAATGAAAAAATCTTAAAGTGGCTTATAGAGCACGACTAACAATAAAAGCAAGCCAAAATCGGCTTGCTTTTATTTTGTCTAATCTAACTCAAAACTCAGTTTACCGTACTTCCCGTTTATCAGTTCACTAACAAACAGTTCGCACGCTAAATTAATGTTGAGCTGATTCGATTTTTGCAACAACTTACGACTGATAGCAAATTTATGTAAAAACTCAGTGAAGTCTGTACCTTCATATGGTAACAATTGCGGATAATGTTTGCTTAAATAACAAAAAGCAAACGCACCTACATCTTCCAAAGGGACTACTTCCCTTTTAATAACGTTAGTTAAAACTAACTTGTAACCAACAGCCATTTCATCAATTCGTTTAAAGAAGACTCCTGGGGTATCGCTCAAATAAAACTCCGAACTTATTTGGTTTCAACTCATTGACTTGGTAACCCCAGCCCGATTAGCTACCTGTAAGTGATTTTTATTTAGTAACAAGTTAATTAAACTGGATTTACCAACGTTGGGCATTCCAATAACCGCTAACCGAAACTGGTGCACTAATAAACCTTTGGCTTTTAACTGATTCTTTTTTGCTTGAAACAAAGTTTGCAGTTTCTTAATAACTAAGCGTTTAAGTTGCAAACCTTGCTTTAAGGTTCCCCATAAAATATTGGAGTTAGCCACTGTTTGTGCTAAATCAGCCTTTAAAGCTAAAGTGAGTTTCGGTTTATTGGTAAAGTAAGCCGTAATTTCGGGGTTTTGGGTTAACGTTGGTGCACGCGCATCAACTACCTCAATTACACCATCTAAACTACTAGCTAGTTTTTTGAGTTGGTCATGGGTCTTCTTCATGTGACCCGGAAACCAGTTAATCTTGACTGCGGAGGTAAATGTATCCATCGATCCCTCGGATCCTTGTAATTGATACTAAACAATCTTTGTCAATTTTCCGGATGATGCGGATGAGGTTAGGAATTTCCACATACATAGTTACTGAAACTATCATCCGTTTTTTGGCTAATGAGTATCCACCCATCGTTTCTTGAATGGACAGACTGTGAGTCGCTTTATCGTTGAGCAGAGCCAACCTCACTTCTTCAATCTTGTCAGTAAAGACCTTAATTTCAGCAAAGTTATACCTTGGGAAAAGGTGACTCACTACTGTACCGGTAAATAACACCGAAAAGAAAGTGGCGATTAAGTTGGGTGAAAAGAAAAGGTTAACCTGTCAAGCACTTTTTTTGTAATCGGGAATATCTTGGAGCACAATACTGCCAGCAACAAACGAACCCATTAAGATCGCAATGATCAAAATAAAGGTATTGACATAAAAGAGAATTGGCCCCACGGAGCGGTTTTTCTTTCTCGCAAAGTATTGCGTTAGGAAATCGGCACCCCCAGCACAAGATCCCAAGATGTAGAGGATTGCTAAACTCGTACCGTTAACAAAACCATAGATCCCGGCATAAATAAAGGTCGAAATAATGACATTGCCTTGTGAAGTATCATTTCATAAGAACGGTACAAATAACGCGCTTTGATCAACACCTTCAGCTTTTTTTGCAGCTTCCCAAAATTCAGTGTGGTGCACTGCGGACAACATCGATGGCAATTGATTAGCCCCCGGAATAATACTAAAGATAAAACAAAAGACGTTGGAAGCGACCACGTAATGGGTTGACAGAATGGTGAAACGTTTCCCAATTTTTTTGTAAGAAAAAATAATCAGGGGAATGTTGACTATTACATAAAACAGCCAGTAAAAGATGTTGAACACTAGGTTGCGTTGTCCATCTTCGAGGTTCTTCAACAAGACAAACAGTAAACGCGCAAAACCTTGGGTTAGCGAACTAATCCCAAAGCTGTACAATCCCGTTTGTTGGATAAACACCAGCAAAAAGAAGGCGTTCACTAAAGCCAGGATGTAGACAATTGTGTACTTTAAAGGTTTTTTAGCCTCATAAAGGGCACTAAACATCAACAGCGAACTAGTAATCTTGGCCCTTTTAGAAGCTGAAGTAGTTTCAGTTTTGTCTTGATCCTTCCTTGTGAATCACCTTTTCAGTCAATCCATGGTGAAAAAGTAAAACTATTGTTTTCGCTCTTTAATTTTGGCAGCTTTACCAGAGCGCTCACGCATATATGAAATGTAAGCGCGTCTTACTTTACCCCGTCTTTTCAATTCAATTGAAATATTAGGGTTATGAATTTGGAAGTTTTTTTCAATCGGAATACCATCAGTGGTTTTACGCACAATGAAGGTTTCACTAATACCTTTACCACGACGGCGTAGGACAGTTCCCGTAAAGTTTTGGATTCTGACCTTTTCCTTTTCTTTAAGTTTGATGGCAACATTCACTTCGTCACCAGCGCTAAACTCAGGCACATAAGCCTTTAATTGCTTTTGTTCTACTAAATCTATTAAAGCTTGTTTATTTATTTTTTTCATCTTTCTTTGCTAAATATTGCTTATACAAATCAGGACGGTATTGAGCCGTTCTGAGCAGTTGTTGTTCTTTGCGATAAGCCTCTATCTTTTTATGGTCACCTGATAATAATATTTCAGGTACCTTATCCCCGTCTAGATCATATGGCCTAGTGTAAGCGGGGAAATCCAGTAAGTAGTTGTTAAATGATTCGTGGATTAAGCTCTGATCATTAATGACACCCTTAATTAAACGAACAGTGGCATCAATCACACTTAAAGCCACTAACTCCCCACCACTCAACACAAAGTCACCTAACGAAATAATTTGATCGACATGTTTATACAAGCGGTAATCAAAGCCCTCATAGTGACCACAGATCAAAATGAGGTGTTCCGTTTGGGTTAAAGCTTGCGCCATGGGTTGGGAAAACTGTTGTCCCTCAGGTGATAATAAAACTACTTTCGATTGTGGAGTTCTACAAGCCTTTAAAGCTTTGAGAATTGGTTCAGCTTTTAGAACCATCCCGCCACCTCCTCCGTAAGCCATATCATCAACCGTTTGGTGTTTGTCCTTGCAATACTGCCTTCAGTTAATTACTTCAAACTCAACGAGCTTTGCTTTTTGAGCTTGGGCCATAATCGAAGCGTTTAAGTAAGGTCAAACTACCTGTTCAAAGAGGGTTAAAACAGTGATTTTCACACTTACTTGGACTTAGCTACTGGCTTTTTCTTACTTTCTACAAACTTTTTCCAAAGACCAGTCTGACTAAACAAAGAGCGAACTGTATCAGTTGGTTTTGCTCCCTTATTGAGTCATTCCAAGGCCACAGCTTCATCAATCTTGCACTTATTTTCTTTTAATGCAGGATTGAGATGGCCAATTAACGCAATGTACTTACCATCACGTTTAACTCTGGAATCAACAGCTACTATTCTGTAGGTTGGGTAGTGAACACGACCCATGCGCATTAATCTTATTTTTACCAATCAAGTCTCCTTTTAAAATAAAGCGGATAATTATACATTGCCGTTAGGTAGTTTTACCTAACTCTAAGAATAGCGCTCAATGAACTGGACAATCCGACTCTTATGTTTTTGAGCGAAGTATTTATCCATAAAGAAGTGGATGTGTACTTGGCCAATCAAAACGTTAATCAATCCAGCCAGAGCAGCGTTAGCCGTTAATAACCAGATAAAGATTTCAATGTTTTTTGTTTGTAAGGTAATTGCTTGAAAGATAAACAACAAGGGTACAAAAACAATAACCCCTTGCATTAAGGAAACTATTCACGAATACAACACCCGGTTGTTATTTTGGAAGTACTGTTGACTGGTAGCGCCAATGGCCACAAAGAACACTTGCGCTTGCACAATTAAGGAAAACTGATTAGCGAGCATTAAGGTAGCTGCACTTGTATCAAACAGGTGCACTAGGATTTCTTTGCCCAATCCAACTGCCAGAATTAAATACAGCAAAGCAGCAAATACCAAACACACTAACAACGTATAAACATTAATCCTTTTAATGTCAGCCAACTGGCCCTGACCAAACTTGTAGGATACCACTGTCCGCACCCCTTGGAGAACACCAAAGATGGCGGCTGAAGTTAGGTTAGCAATTGCAATGGGTCCCGTTAGTAACACCAAGTAAAAGGTATCACTTTGGTCCGTAAGCGTTTTGGTCAAATTCACCAAAAAGCTTTCGTAAAAGGTGTTGAGAATTGAGAGCGAACCATTACGGAAAAAGGAGGCTAACCCCACCATTGAAATGACCAAAAAGAGGTTAAAACTAACCCGTTTAAAGGAAAAGTCCCTCAACGAAAGGTAGGTTAAACCACGTTTATTTAAACTAATAAGGTACACCACATAAGCCATAAAGGTAATGAAGTAAACCAAAATCGCAGCCACCGCGGAGCCCACAACCCCTAAAGTGGAAAAACGCACTAACAAGAAAACAAACAGGACGTTCAGTAAGTTCGATAAAGGAGGCACAATTGCAATAAACAGTTGCCGGCCCTCTGATTGGCCTAAGTAAAAAAACAAGCGGCTCAACATCGGTATGATGTTAAAGCCAATCAAAATGTAAACGTACTCTGAACCAATCGCAACCGCCTTTTCGTTAAAGAAGTGTTGAAACTGATCCGCTACTTGTACGTGGTGTTGTTCATCGACTTTATCCAAGTTAAACTGCAACCACTCTTTGGCAATCGCTAGCACAATAATTTGGGTCACTAGGGCAAATAAGGTAGTGGAAACTAACCCGGTATTCCAAGCATCCTTAATCTTTTTCTCATCACCCTTACCAATGGTTTTGGAAAAGATCACCCCCGTACCCAACGGCACAAAGATGGTAATGGCGTTGAGAATGACTACAATTGGCTGGCTAATATTTAAGACTGTTTTGACTAATTGACTAGCTGTTAGTTCCGTGTGGTTGGGAATGTCCCCACCCTTATAAAACGCGGATGCTTTAAATTCCTCAATTAAGGCCTGGTCAGTAAAGATTGAATCGGGATTAAGTGGTCCATGCGGGACAAACTTAATCACCATAATTTGGTCCACAAAGACATAAGCAGCGGAAAACAGCGCAAAGAAAAAAGTCGGAATAGCAAAGCGCAAGATCGTTAGAAACAGTCTTTTCGATTCAAAGGATTTTTGGATGAGGACTTGCTTCGCTAGACGATCTTGTTGGCTATTCACTTAGTTAGATTCCTTAAATAATTTGAGGTATTCACCATACCCCGCCGATTCCATTTCCGCAAACGGAATGAACTTTAGAGCAGCGGAGTTAATGCAGTAACGCAAACCACCATGCTCTTCGGGACCATCACGAAAGACGTGCCCTAGGTGGCTGTTGGAGTTTTTGGCGCGTACTTCGGTGCGGATCATCCCGTGTGATTCATCGCGGTAGTTAGCAATTAAGTCTTGACTAATTGGTTTGGTAAACGCTGGTCAACCACAACCGGATTTGAACTTATCAGTTGAGATAAACAACGGTTCACCGGACGTAATGTCAACGTAAATACCCTTTTCGTAATGGCGGTTATATTCATTAGTATAGGGCGGTTCGGTGTGGGCGTTTTGGGTAACGTCGTACTGTAGTTTAGTTAAGGTCCGCTTTAGTTCCCCGTCACTCTTTTTTTGGTACTTACTCATGCACTGAATTTTTCACTATTATTTAAGTAAACATCAAGGAAACTTTTGCCTATTTTGATGTGCGACTTAATTTCAATTATACCCTGATTTTTAGGTTTTAAATTGAGCTCTTTTTCCGAACAGAGCATTCCCATGGAATCATACCCCGCAATTTTAGCCTTCTTGATAATCGTGCCATCAGGTAAAACACCACCAACATGGACTAAAACAGTTTTAAGGCCAACCCGAACATTATCCGCCCCACACACGACATCCAAGGACTTATTGGAACCAGTGTTGACCTTACACCTTTTCAAATGAGTGTTGGGAATCGGAATGGCCGAAATGACCTCACAAACCACAAAGGGTACCTTTTTGGCTAAGGGTGTTAAATCATAGTTCAACAGCTCACTAATCTTTTTGACCAACTTCAAAGAAGGGTAATTCAACCCTGCGGTAAAGTGATGGGAAATTAATCCCCTGCGAAAGCTCTTTTTAATGTCAAAGAAGTTAAACCCAGCAATCTCATTGGCTTCATTGACAAAGAAGCTAAAGTTCTGGTTCTTTTCTTGGCGCAAAGTAGCTTGCGTGCGGCTACCAATTAAACCAAACATACAGTTCTTAAGTGTTTTCTTGGGACAAAAAATGGTGATAAAGTCCTGAGAAATATCAAAAAAACCGTAACGCATTGTTAACTATTCGCCTTGCAGCACAATTACATAGCTGTTCAATCCCGTGTGGGTACAAATGACCGGGGACAGTAAGCTCTGTTCTATATTAATGGAATGATCCAACAGTTTATCAAACTCTTGGACTAAATTAGTGTTGGTCTGTTCGTCAAAGACGGTGGTAAGAAAGGCACCCCGTTTCAGTTTAGCGGAGTGTTTTAACAGCTGTTTCTTTAAAAACTTGACTGCTAGCTTATGCGCATCATGAGCCGAACGCGCTTTATCAAAGAACTGTAACTTACCATTTTCACCTAAAAAACTAATCAGTAAGTGAAACTTTAAGCTCTTGATAATAAAGGATTTAAGATTGGAAAGCCGCCCGCCTGCCACTAGTGGTTTGGTATCAGTGACAAACAACACTGCGGCCGTTTTCTTGCGGTACTGCTCCACAAAGCGATCCAAGCCTTCCCTGGAATAAGGATTGGTGTTCAACCAACCCTTAATGTCATTGATTAGCCAATCAATGATCATTTCCACCGCTCTGGCATCCAATACATGGAACTTCGAAGCGTAATCCTTTTCAACTAACTTCCAGTTCGCGTACGAAGCGGAGATTTCCTTATCAATCGGAATGCCGATAATCAGGTCATACTGCTCCACTAGTTCACTCACCTTAGCGCGCACTATTTCTTCAGTAGTTTGCGATGTGGAGAACTTCACTCCCTTCGGAGCGTTAGCCATCTTGTCCGTGAGGGTTTTTAAATCAATGTCAAACCCCGAACTAAAGGTGGTTTCAGCTTCAGGAGTTGATTCAATGATGAAAAGCGGTAGCACATACAGATGTTGTGCCTGGCTTTCCTTGAGGTTACTAACAGAGTCAACTAAGAATGCGACTCTCACTATTCAAGCACTTCCGTGACTGAACCAGCACCAACCGTTCGACCACCTTCACGGATGGAGAACTTACTACCTTTTTCACAAGCAATTGGTGCAATTAGTTCAACTGTAATCGAGGTATTGTCACCTGGTAGCACCATTTCGGTGTTTTCTGGTAGGGAAATCGAACCAGTAACGTCTGTAGTACGGAAGTAGAATTGGGGACGGTAACCGTTTAAGAAACCGGTGTGACGACCACCTTCTTCCTTCTTTAAAGCATAGATTTCCGCTTTAAATTTCTTGTGCTGTTTAATCGAACCTGGTTTAGCTAACACTTGACCACGTTCCACTTCTTTACGGTCCACACCACGGAGTAATACCCCAGCGTTGTCCCCAGCCATTGCTGAATCAAGTTCCTTTTTGAACATTTCGATTCCGGTAACAACTGCTTTACGGATTGGACGTAAACCAACGATTTCAATTTCTTGACCTACTTTCAATTCACCACGTTCAACCCGACCGGTAACCACGGTACCACGGCCAGTAATCGTCATGGTGTCTTCGATTGCCAACAAGAAGGGTTTGTCCACTTCACGTTCAGGAGTTGGAATCCATTCATCAACTGCATTCATTAAATCATGGATCTTAGCTTCCCACTTAGGATCACCTTCAAGCGCTTTAAGTGCAGAACCATAAATAATAGGGGTGTTCTTGCCATCAAAGCCGTAAGAAGTTAATAAGTCACGTACCTCTTCTGCTACTAACTCTTGCACTTCTTCATCAGTTGCAATGTCACACTTGTTTAGGAACACTACCATGCGTGGCACACCCACTTGGCGGGCCAACAAAATGTGTTCACGGGTTTGGGGCATAACACTGTCAGTTGCTGAAACTACTAGAATGGCACCATCCATTTGTGCAGCACCAGTAATCATGTTCTTAATGTAGTCAGCGTGTCCTGGACAGTCAACGTGAGCATAGTGACGCTTGTCAGAGGAGTACTCCACGTGAGCGGAGTTAATCGTAATTCCCCGTGCTTTTTCTTCCGGAGCCTTATCGATTTGGTCGTAACGAGTAGCAGCTGATTTACCTTCTTTTGCTAATACAGTACAAATAGCTGCTGTTAAAGTTGTTTTACCGTGGTCAATGTGGCCAATAGTACCTACATTAACGTGGGGTTTAGATCGGTCAAATTTCTCTCTTGCCATGTGTTTGAATTACGTCTCTAATTTTACATAAGTTTGAGATAAAGCTGTAGAGAGGCTTATAATTCTTGGAGTAGTGTAATTCATATGGAGCAAAATAATTGCAAAATCAGAACTTGATTTGCCAAACTGGCGTTAGCACAAAAGGTCTTCTTGGGGGTAATTCCCTTATTTTTTATTTGTTTTGTTTTTGTAATTGCTGACATTGTCATCAGTTTGCAAAATAAAGGCCACATTATAGAAGAGATAGATAAGTTTACTAACCAGAGCAATGTTATGTTGCTCATTTATGCATGTTGGTATGTAAGCAAACCAAAGAGCCATTATCTCAAGAACCAGCAGTTCTTTTTGAGTGCCTTTGCTTACATCATCTTTACTTTTTTAGGCTACAACGTCATTTTGGCGGCTTCACAACAAGCTTACAGTGATAAGGATGCTTATTCCTTGGCAAGTTCTGTCTTTCTCCATGTGTTGGCACCAATTGCTTTCTTGGTTGCAGGTATAGTTAAGATGAAAACGGACAAGGATGTAACCTTTAATCATTTTTGAAAAAGTTTGGGTTACTTCATGATCTATCCCTTAGTGTATGGGTTGTACTTAGCGACCATTCCTTATGTAAGGGGTCATTATGTGAGTGATGATGGTAAGTCTACTACCTATGTGGTTTATGGGGAGATTACCAACACCAAGGATAACCCGATCGTCGCTTGACCAGTAGTAATTTGTTTCCTTTTCATTTACTTTCCCTTGAGCTTCTTAGCGGTTTATGCCTTGCAATGTAAGTTATTGAATAGACCTCTAAAAGCACAATTTAAGTGTGCAACCAATAAATGCCCAAAATAAGAAAAGCCGTTATTCCCGCAGCCGGACTGGGTACCAGATTACTCCCAGCCACTAAGGCCATTCCCAAAGAGATGTTGCCCTTAGTCAATAAACCCACAATTCAGTACATTGTGGAAGAAGCAGTAGCGAGTGGCATTAAGGAAATCCTAGTAATTGTGTCTTCTAAAAAGGAAGCGATTATTGACCACTTTGACTATGACTTTATCTTGGAAAATGCTTTACTCCAAAAGCACAAAGATCAAGAACACCAAGAAATTAAGGATATTGCCAACTTAGCGCACATTTACTTTGTCCGCCAAAAACACCAACACGGTTTAGGAGATGCCATTTTGCACGCCAAGTCGTTTGTTGGTAACGAAGACTTTGCCGTCCTTTTAGGTGATGATGTCGTGTTTGGTGAACAACCGGCATTAGCACAATGTATTCAAGCTTATGAACAAACAGATTGTCAGGTAATAGGCGTGCAAGAAGTGCCCCATGACCAGGTCAACAAGTATGGCATAGTAACCCCTGAAGCTAATTGACAAAAACAAGCGTTAGTCAAAATTTTGGGCATGGTGGAAAAACCAGCTGTCAACGAAGCCAAGAGCAACCTAGCCATTTTGAGCCGTTACATCCTTAAACCCAGTATTTTTACAGCTCTTAAACAAGTACCGTTTGGTGTTGGGGGTGAGTTACAGTTGACTGATGGGTTGAACTATTGTTTACAGCAAGGGGAACCCTTCTTTGCCAAGCACTTTGGTGGTACCCGGTTTGATGTCGGTACTAAAAATGGTTTTATTAAAGCGAACCTGTACACGGCTTTAAAAACCGATGCCATTACTAAGGATGAAGTCTTGGCCATCCTCAAGGAATTTGCTTAAAGCTTGGCACCGTTCAAGGTTAAACCCAAGAAGTTTTCTGGTTTAATTAAACGGCTAAACGGACACATGGCATGCGCTGCCGTAATGACTGTTTGCGCTGTGGTTTGGTCCACGCCCGTAATCGCTAATTCAACACCGGCTTTAATGTGGAACAGACCGTCCTGGTCATGCAGTTCGACACTAACACCCACAATAGGTTGAGTAGCTAATTGCAGTTGGTGCTGTTGTAAAACCACACGCACAGCTTGCGAAAAACAACCAGCATAAGCTGAAGCAAACAGCTGTTCGGGGTTGTTTTTGTCCTGGTGAGTAGCACCAGGTTTAGGAAATGCTAAACTAACGGTAAAACCATCAACGGTTTGGACAACGCCTTCCCTGCCGGCGCTAGCGTGTGCTGTTGTTTTGTAAATTACTGCCATTGTTACTGATATTATATTTAGAAAGATGACAAAAGATCTCCTAGCACTTTTAAAAGAACGGGGCTTATTTGTACAAGCTAACTTTGAAAAGGAATTAAAGCAATTATTAAACCAAGGATCATTTGCTTTCTATGTTGGCTTTGACCCCACAGCACCCTCACTCCATATTGGCAACTATGTGCTGCTCCATGTAGCACAAATCTTCCAAGCAATGGGTCATATTCCCCACGTCTTGTTGGGTAGTGGGACGGCTTTAATAGGTGATCCTACTGGCAGAATGGAACTGCGCCAGATGATGAGTCGTGAAACAATTGCCGAAAATACGCGCAACATTAAAAAACAAATTAGGCGCTTTTTGGGTAGTAATGTAGTGTTTTGCCAAAACGAGACCTGGTTAAAGAAACTCAATTACATTGAGGTCATCAGGGAATTGGGACCCTGCTTTTCGGTCAACAAGATGTTAGCGACCGATGCCTTTAGTGCTCGTTGGGAACGCGGTTTAACGTTGATGGAATTGAACTACATGGTATTACAGGCCTATGACTTCTATTATTTAAACCAAAAGTATGGGGTGCAACTGCAAATAGGGGGCAGTGACCAGTGGGCTAATATCCTAGCGGGGGCTGATTTAATTAGACGCAAAACCCAAAAACAGGTGTACGGCATGACCACTAACTTATTAGTGAAAGCTAATGGGGAAAAGATGGGTAAGAGTGCTAGTGGAGCTTTATGGTTAGATCCACAAAAGACCAGTCCGTATGACTTCTACCAGTATTGGATTAACCTTGATGATGCTAGTTTGCAAAAGGTTTTCCTTATGCTCACTAAATTAGATACCAAGGCAATTGAAACCCTCTGCAACTTGAAGGGCGCTGCCATTAAGGAGGCCAAGGCAAAACTAGCCTTCGAGTTAACTGACGCTATTCATGGTACCAAGGCAGCTTTAGTCGCCCAAGCGAAAAGTGCCCGAATCTTTGCCTTTCAACCTGATACTGAAACCAAAACCGTGCGAGCTGGCACCAGGTTAGTGGATGTGATTGTTGATTTAGGTTTAGTTGTATCCAGATCAGAAGCGCGCCGGGTGATTCAGCAAGGTGGTTTAACGATTAACCAAGAAAAAGTGACTGATGTGGAAATGGTCCTACAAGCGAGTTCCCAACCACTAGTGATTGGTAAGGGCAAAAAGCGTTTTGTAACGGTGCAAGTGATAGCTAACACCAAATAAAAAACCACCTTTGAGAGTGGTCTTTTAATGAACTTAGTGTTATTTCTTAGCCTTCTTCGCTTTAACTGGTTTGCGCTTGGAATTGGTTGTGGGTTCAGTAACTTCAGCCACAACTGTTGTTTCTGTTGCACTGGTAGTTTCAGCGTTGTTGAAATCACCAATGAGTTTGGTCTTTAACAGTTCCACTACCTTATGGAATGAATCTAAGGTAGCAGCGTCATAACCAGCTTGACGGGTAATATTGAGTTCTAAATCACCCACAGCTTCCTCCATTCTTAAAGAAGTATCCATGAAGGTAATCGGTACACTCATCATGAGGTAAGTGTGTGTGGTATTGTTGTAAACGTTAAAGTTAGCATACACATTAACCGGGTTAAACAACACCTGCTTTATCATCTCCTTATCTAACTGATTAAAGTTAAAACTAGCGTCTTTTTCAGTGTAGAGCAAAACGTTGTTCTGGCGGTTACTTTCAGTTAAAGATTCGTAAAAACCAGTGTTAATTAAGGGGAAATCGCTTTTAGTTAAGAACCAGTGTTGGTCACTAACAGCATCAATAATAGCGACTAAGCAAGCCACCTTTTCATTTTTGGAAGTACTTCTCTTAATGGCACTTAACTTAGCAAAGTTAAGCGTTTGACCATTCTTGTACTGTACCTTAAATTGCTTGGGAACTAACTCAAAGTTATAGATGGTAGCAGGGTAAAAAGTCGGTAGAATATCCGCACTAATTGGACCTTTTTCAAACAATTCAAATTGCTCAAACTGCTTGTTGTTTGCCATCTTTTGTAACCACAAATCAGTACGATCTTGACGGTTGACCAAATGAATTAACTGACTGCGTTTGTACAAAGCTACAAAGTTAATCACAAAGCTCAACAGCAAGGTAAACCCGCCTAAGGCAATTAGGAAAAGAGCTGCCACTTTAGGAACAATTGAACCCTGACCAACATAGCTGTAAATCCCTAAAGAAAGGGCAATTAAAGCAAAGACACCACAAAAACCAACAACTCTTGTAAATTTATTGAACTTTTCAGGTGTTAAAAAGAACTTTTGAAAACGAGTTAGCTCTGGCATGCGTATATAAAAGATTGTAAGAAAAGATAAGTTAAACACAACGCTGGAGTTGTTTGCCCTACAGAACGTTTCCCTAAAAACTGCGCCTTGCCAAACTGCGCCCGGGTTTGACAAAGCCGTTCATACAGTTCAACTAACACCCCATCGAGCTGCTTGTACTGCCTTTTGGTGCGGTTTACTAATTGCAAGAAGCTTTTACAAAAGTAATACAACAAATCCCACACCCGTTTGTGACCTAAAGTAATTGCTTTAGTTTGTATCAATTTTTCGGTGTGTTTAACAAGGGTTTTCACCACGGTAATCACATCCTTGTTTTCTTCTAACAACAGGTATTCTACAGCCTCCCACCAAGTTAGCACAGCATTGGTTTCACAACCCAACAAGTTGGAGTGTTGCTTTAAGACTGCAATAAAGTTTTGCGGGGTTTTATCAACATCGTTGAGGGTGGTAAAACACTGAAAGCTATAGGTGCCCAAATCGGGTTGCAAGTGGTTGAGCTGCTTTTGCAAGTGCGCGAGGTTAACTAGGAGCTGATCGACAAAACTAGCAAACTTAACTGTTTGTTTCACTGTCTTTTTCTGTATCCTTCTCAGTCTTCTTATTTAACTTAGCTTCCTTGGCTTCTGCCTTTTGTTTGGCTTGCTGTTCTTGCTTTTGCGCTAGGATCTCTGGAGGGAGTTTAGTGTGTTCGTGAATGTAATCGATGTCACTCTTTAAGATGGTTTCGGCAATCAACAAGGCTTCAACTAACAATTCCAACTCCTTACGGTTCGTTTTAATAATGGTACGTGCCTTCTTGTACTGTTCTTCAATAATGGCGTTAATCTCAAAGTCAATATCCTTTGCTGTTTGCTCAGAGAACAGTTTGGTACCTGGTGGGACGGTACCTTGACTAGGTACATATTGCACTTGCCCTAACTTGGACATTCCTAACTGGGTAACCATGGCACGGGCAATATTGGTCGCCTTATAAAAGTCCGAGGAAGCGCCAGTGGTAATTTCTAGGGGACCGTAAATTTCTTCCTCTGCAGCACGGCCGCCCATGGCTGTAGCAATCATCGCTAACAAGTCAGATTTACGCTTTAAATTGAGATCGCCACTCTTAGGGGTTGACAAGGTATAACCACCCGCTTGACCCCGGGGAATAATGGTAATCTTTTGCACTTCATCGTTACTGTGAACATGGAGGCCAACCAAAGCATGACCTGCTTCGTGGTAGGCCACTAGTTTACGATCAGCATCACTGACCACCCGAGATTTTTTGGCAGGACCAGCTATGACCCGGTCAATGGCTTCATCAATGTCGTTCATGTTGATCGTGGTCCGGTTATCACGGACTGCTAACAGGGTAGCTTCATTAATGACGTTTTCCAATTGAGCCCCACTAAAACCAGGGGTACGTTTAGCAACATCCAATAAGCTAATCTTGGAAGACAAGTTCTTGTTCTTGGCGTGCACTTGTAAGATGCCTTCGCGTTCCTTAATGTCGGGAAGGTTAATCTGGATGTGACGATCAAACCGACCTGGGCGTAATAACGCATCATCAAGGACATCCAAGCGGTTGGTAGCAGCCATCACCACAACACCAGTGCGGCTAGTAAACCCATCCATTTCCGCTAACAGTTGGTTTAAGGTTTGTTCGACCACCGAGTAGGAGGACAACTCCACGCGACCCCGTTTAGAACCAACCGAGTCAATTTCATCGATGAAGATAATACATGGGGCCGCTTTCTTAGCCTTATTGAACAAGTCCCGTACCCGTTTGGCGCCCACACCAACCAGCATATCTTCAAAGCCAGAACCCGTGGACTGGAAGAAAGGCACACCAGCTTCACCAGCAACAGCTTTCGCTAACAGCGTTTTACCCGTACCAGGAGGGCCGTAAAGAATAACACCGCGTGGGGAACGAGCGCCCATTTGGGCATACTTCAAGGGGTTTTTGAGGTAATCAACAATTTCCAGTAACTCGTGTTTTTCTTCTTGGAGTCCCGCAATGTTGTCAAACCTCACCGAAGACTTTGCGAGTTTAGCTTGGGTCTTACCAATTGAGAAGATGTTGTCCTCCTCACTGCGACCACCACCAGAGATACCGCGAGCAGAACGTCAAAAGAGTAAGAAGAAAATGACAAAGATAATAATTGGTAGTAGGCCAAAGAAGATGTTGAGCACATCACGTGCTCTCATATCAGGAGCAACAAAGGTACCGATGTCATGGAGACCTTTAATAGAAGCTAATTCAGTGCCATTGTTACTTTTTTGGTCTTGGGTCATCATTCCATTAACTATGGCCTTTCCGGTTGACTGATTAATCGAGAGGTTTGCTATATTAAAGATAGTTTCCCCACTACCATTAGTAGCTTTGTGGGCAGTTACTACTATGGGATTTATCCCTTGGAAACTAATGGAAACCTGCAGAATGTCATCGGTGACATAGGTGGAATTATTAATTCTTTGGAAGGTGTGTTTGCCTGATAAGCCTTTGATTTGAGCCGAAAGTGTAGTTCCACTAAGCTCCCACTTTTCAATTACAGCCGTGGTAGCCCTTGGCATTAGGATATAAACCAAAATACCAATAATAATGGCTAGGATAACCACTCACCAAAAAATCTTTCACGCTGTGCGTTTGGGAAACTGCGGTTTTTCGCTCGTAGTCGCCTCGTTAAGTCCTTTATTTTTTTTCATTTGTTGTTGTTTGTTGGGTGATTTGACTGGTTTTTATTCTAACAACACTAAGGGTAACTAATACACCCACTGCTGCAAAACCAGCCAAAGTTAGAAAGGTAATTAAGATGATTTTTTTGTGCTTTTTTACCAGGGAAACCAACACACTAAACCATCGCATAAAATTCTAAATAGCGCGACTTAATTATCTTCAGGATCAAAGACACCAACATAGGGTAAATTGCGATAAAAGCCCTGGTAGTCTAACCCAAAACCAACCAAGAATTCGTCTTGGTTTTTAAAGCAAGAATAGTCCACCTTAATGTCAGCTTCCCTGGGTTTGAGCTTTTCAATGAAGCTCACTAAGATCACACTTTTGGCCTTTCGGGTGTGGAGTAAGTCCAATACTAATTTAATAGAACGACCACTGTCGACTATGTCTTCTATTAAAAGAATGTCCTTGCCTTTAGGGTCATGGGACATATCTAGGACAATCTTCGGTGCTTCTTGTTGGCGACTGCCACCATGGTAAGAAGCGACTGCCACAAAGTCCAGTTGTAAATCAAAGGTAAACTGACTAATAAGTTTGCCCAAAAAGGGAATACAACCCTTCAAGATCCCTAATACTATGACCTGTTTACCAGCAAAATGTTCATTACACCATTTGAGCGCAGCGTTACAACCCGCTTCAACCTGTTTTTGATCAATGATGATAGATTTAATGCCCATTACTTTAATCTTTGTCGTTCCACTAACACGACCGCTAAACAACTAATTTCATTCGGATTCAACGGTTCACGTACCTTGAGGTTAATTGTCAGTTCGGTAATCCGTAAAGCCGTACAAATAAAGTCAAAGATGGCATGCTTATAGTCATCCATCTTTTGTTGTGAGGGAATTTCTAAGGTGATATCAATGTTATTGATGTACCAGTTCTTGCGAATAAAGTTGTAAGTCCGTGCCAAAATACGGGGCGCTTCTTTACGCGGATTACCCTTTAAAGGGATCTGGGTTTTTTCTTTATTGAAGACAATTTGTCCATCACCTAAGGCAGTAGCACCAAATAAAGCATCAGCTACTGCTAGCTGGATAACGTCACTGGCGTCATCACTAGCATCGTGGTCATAAATGTACTTGGAATCTTCTATTTCCACCCCACCTATTCAAAATTTATTCTTCTGTTCTGCTCTCGGTTTAATGCGGTATTTTTTCTTACCTAGGCCAACTCTAAACTGCATCGTGGTATCTGTTAATTATACTTTTGCCACCCCCTTTTCCTTTTATGTTGAGAGCTAAAAAAGACAAAGCTTGGCAGCAGGTTTTATTTAGATCAAATCTCAATTGGGAACAGTAAATGATTGGTGGCTTAAACCAAATTAAAATGAAGGCAAGCTTGAATAAATTAGTTATGAAGAGTCTTAAAGTAGCACTCATTGGTTCTGGTGCAGTGGGCACCAGCTTTCTCTACGCTGCCATGAGCCGTGGTTTAGCGAGTGAATACATGGTTATCGACATTAACGAAAAATCCCAAGTAGGGAATGTTTTTGACCTCCAAGATGCTGTACCATCATCACCGCAATACAGTAAGGTAATTGCTGGTGATTACAAACAACTCAAAGATTACGACTTTATCTTTATTGGTGCTGGTCGACCCCAAAAGCAAGGTGGGGAAACCCGGTTACAACTCCTTGAAGGTAATGTCGAAATTATGAAGAACATTGCCAAAGCCGTGAAGGAATCGGGCTTTAAGGGCATTACCTTAATTGCTTCCAACCCAGTCGACATTATGGCTTACACCTATTTAAAGGTAACGGGGTTTGAACCGAATAAGGTAATTGGTTCTGGAACCTTACTAGACAGCGCCCGGTTAAAGTTTGCGATTGCTGAAAAATATGGTATGAGCAGCAGGGATGTCCAAGCATATGTGTTAGGTGAACACGGGGATAGTTCAGTAAGTATTATTTCCTCCGCTAAGATTGCTGGCTTACCACTCAAACACTTCTCGAAAGCAAGTGATATAGAAAAAGAATTTGCTGAAATTGACCACTTTATTCGCCGTCGAGCTTATGAAATCATCGAACGCAAAGGAGCCACCTTTTATGGCATCGGGGAAGCAACTGCCGAAGTAGCAGAATTAATTCTCCGCGACACTAAAGAAGTACGAGTAGTAGCATCGTTAATTAATGGCCAATATGGTGCTAAAGATGTAATGTTTGGCACCCCTTGTGTCTTGGGACGCAATGGTGTGGAAAAGATCTTGGAAATTGAATTGTCTGCTACAGAAAAAGCAGGCTTGGACAAATCAATCCAAGTATTGAAGGACAACATTAAGTTAGCTAAACTATAAGAATTAGTTTAACTGTAGACATAAATAAACTAGTTAACTACTAGAACAAAACAAGCATGCTGACCCATTGGGGTTGATGTGCTTGTTTTGTTTATTAAAAAATAGGCAAACTTTTTGGCGTATTTGTCAAATTCATTCAATTTATTAAGTGGGAGCAGGAAGACCGACTTTTGTTGAAAGTTTTTGCTGCTCCCGGCCAACTAGAAAAATATTTAAATTCATACATCTTAGTGTTTACAACTCAAACTAATATTCTTCGTTAGTTTTCAATATCGTTAAAAAATATTTTTTAGCCGTCTGCTTGTCAACTTGAGCAGATGGTTTTTTATTTATTTGAAAAGAATAAAAATACCAGCAACTCAACCTTAAAGATTAAATGCTGGTATTTTTAGTTTGAGTTGGAATTATTTAGATTCCAATTTGTCTATACGTTTGCCAAAGTCTATTAAGGTATTGTTAATTGATTCCAGGCTTTTAAGCACAAGACTTTCAAAGCGATCCATGCGGGCGTTCATTGCTTCACCCTGAATTCGAAAGCCTTCTCTAACTTCTATTTGGAATTCTTTAAATTCTGCCTTAGTAACATATCTTTGATCAAACTCAACTAAAAGCTCTTGTTTAAAAACTTCTAGTTTTTTGTCCAATTGTTTATGGGTTACGTATCCGCTTTTGCCTTTTTCCATATGGTTTAAATTGCCATAAAAGCCCATGTTATAGCTACCATCAGATAGATCTTCGATTCAGGCCTTTGCTGTTTTAATCTTATCTTTTTTCAAATTTCTTTTGAACAAAGTAATTGAAATTTCGCGTTTAATGGTCAAAACCTCCTTTCTTTACAGGATTTAAAAAAACAGAAAGCTTTATATGAGTGTTGGGCCAAATTTAAAGAGGGTCCAATTTTTCAAAAAATGTTAATCATTTCTTTTAAGCTCCTTTACTAAATAAATGGAAGCGGTTATTTAGAAGTACCAAGAAAATGAATTTTTAAATAGAAATCTGTAAAGAATGAAAATACCAACAGCTAACTGAATGTTAACTGTTGGCGTTTTCTGTTGAAGAAATGTTATTTCTTGTATTCAAACCTATCTAAACGTTCGTTGATGGCTTGAAGGCTCTGAAGCACAACTTTAAAAGTTTCTCTAACTTCTACTTTGAAGTCTCTCAACTCCGCTTCAATGTTTGCGAACCTTCGATCAACCATGACCTTGCATTCCTCAAACTCTTTCCGGGTAACGTACTATGTTTTTGGCTTTTTCACTTTTCGTTGGCAACGTTCCTTAATTATAACATCAAAAGTTCCGTCTGGCAACGGAATCCAATTTGATTTTAATTCAAGAACCTCACACTGACTTCTAAACTTCTTGTATTCCCTTTCGGAATATCATTTGTTATTCAAAGTCAAAACCCCCTTTCTTACTTAAGTAATTAGAAAAAGAAGTAGACACTACCGAATTCCTTTTAGAAACAACAAATGTTGATGCGAGAACCAACGCAACAGATGCCTTTGCAAACGCGAAAGACTTATGCCCATAAGGGCTAATAAAAATGAGTTCATATGAGATTAAAGGGAGTTTTTACACTACCCAAGGTAATTAAATAGTGTTAAAGAACAATATAAAAGCCACCCCGAAGGGTGGCTTTTCCATATACACAGAAAGCGAACTAAATAAATAGGTTGTCCCATTCCGCTAACTGCAGTTTTATTATAAAAGAAATAACTAACCTTCGGAGTGGCTTTCTTTCTATACTGAGCAGGAGGCGACTAAAATAGTTGAATAATTCTGTCTACTGCAATCTAGTTATAAAAAACTCACCTAAAAGGTAGTTATTAACACCAAAAAAGCTTTTTAGAAGCGTTCTTAGTTTCAATTAAATAAACTCAGTGAATTAATTAAGCTTAACCAACTAACTTAGGCTTTTTCTTGAAGATAACGTTTAAGTATCAACTTCATGGTTTTGAATATCTTCTATCAATGCTTAAAGGCGGTAGAAAACGTTAGCCCACTTGTACAGTGCATTTTCAAAGAACAGTTCATTACTACGCTTTACCAACATACTGATTTCACTGAGGTTAGTGTGCTTGTTTTGCATAGTTTTAATTTTGAGCTCATTCTTATGACTACTTCAACCCTCATAAAAGCCCTTAAACTGATTGTTAAACTTCGTAATAAAGTACTTAGCATCCTTGTACTTGCTCGCCTGTTTTTCAAAGTCTAAGTCCCTCTGTTCCTTGGTTTCGTAAAACTTAATTTCGAACTTCAAACTGTTTAAATAACTGTCCAACAACTTCTTTAAGATCTTGTCCTTGGTGGTTTGGTACAAGTTAACAAAGAACGATTGGAACACATAGTCATCTAACTTAACAAACGCTGGCAGCATCTTTTTGTCAAAGTGTTCGTTCTTGAGTAGTGGTAAGCACACCTGTTCTAAGGTTTTATAACCGTTGAAGTCAAACTGGTTTTTGTCCACTAAGTCCTTAATTCGTTGAAAGACAAACCAAAGGTTTAACTCGGTGGAAATACTCCTGCCATCGCTGTAGATTGATTGGTACATGTGGTGCCGGGTAATTAAGAAGTTTTCAATTAAGGGCAAGCACTTATCCTTAAAGAAGATTTCGTAAATCCCCTTGACCTTGACACAGTCCATCTCCTTAATAATCGTTTGGTAATCTATCAAGGAATGACTGGTACCCGTAAAGTAGGAATCGCGCAACAGGTAATCCATCCGGTCAGCATCCAAGTCAGATGAAATTAACTGGCGCATCCAGTAGTTGGATGGCTTTAACTCCTTGTTTTCATCAATTAAAGCAGCTACTAGTTTAGGATCAATCTGGTTCGCCTTTAAGATTGATTTAATCGGTTCACTGTTGACAAATAAGGTTGTAACTTCTTCGTGGATAAACAGCTCCTTCTTAAAGTTAGGGGCTTTGGCAAAGTAAATCTCAAAGGCATGGGAATGCGGTGCATGACCAATGTCGTGCAGTAATCCCGCTACTAACACCGTTTGCTTTTTGTTTTCATCAATCGGAGCAAACGCGGGGGTGTTTAAGATCCGCCTTAACAGTTCATAAACCCCCAAGGAGTGGGAATAACGCGTGTGCACGCCCCCCGGATAAAAATGAAAGTTAATTCCTAGCTGCTTAATGTTACGTAAACGGCGAAACGCTTCCGTTTCCACCAACTCATACATCCACTTAGTTTGTTGGTCAAACAGCACTTCCCCTAAAATGGGATCTTTAAAAAAGATTTGTTGCATGAATAAATTCTTGAAGCAGCGCTAATACCTGAGTCTTACCGTGCAGCACCATGATGCCCGCCAAATCAGGACCATGCTCTTTGTTGGTAAAGATCAACCGTAGTGGCATAAACAGCTTTTTGCCCTTCAGATTAAACTGTTCACCAATTTGGGTTAAGGTACTTTTGACATTGTCCTTCGTCCAATCAGGTAGGGCTTGGAGTTGTTCACTCAGTTGTTGTAGGGCTGATTGGAGCTCTTTGAAGAGTTCACGGTGCTTAACACTTAAATGCTGCACCCCTAACTTGGGGGCAAAGGTTTCCTTAATCAGTTGGTTAATTTCAATGCCAAACGCTAGTTGGTTTTTGAACAACAGACTGACTTCTTTGCGCTTGTTCTTGAGGTAATCAAAGTCAATCGTTAAGTAGTTATCAATAAAGTTAAAGTAGGCATTATCACTCAACTCCTTAATGTAGTGCGCATTAATTCACTGGAGTTTCTTAATGTCAAAGAAAGCGGGGGCGCCCACTACTCGATTGACCGTAAAGCTTTCAATTAGTTGGTTGATGGTAAAAAACTCGCGGTTAAAGTCCGAATTTCAACCCAGTAAGGCCAAGAAGTTTACCACCGCTTCTGGTAGGTAACCCTCTTGTTTGAACTGTTCAATGAACTGGATCGTTTTGGTATCACGCTTAGACAGTTTCTTGCCACTCTTGTCGACTATCACCGATAAATGACCAAAGCGGGGAATCCTTTGATAGCCTAAGGCTTGGTTAATTGCTAGTTGGTAAGCGGTGTTGGAAATGTGTTCCGCCCCCCGCAACACATCGGTAATTTCCATGTCATGGTCATCAATGACTACGGCAAAGTTATACGTGGCAATGCCATTGGCCTTCAATAAAACAATATCGGTGAGGGAGTTCCCGGGAATAGTAATCTTGCCCCGAACCTGGTCGTTTCAGGCAAACTCAGCGTCCTGGTTAATCTTTAAGCGAATCGTGAAGGGTACTTGGTTGTCTAAGTTTGTTTGAATGTGCTTGGAGTGTAAGTTGCGACACGTTCCCAAATACTTCGGGGTTTGGTGGTGTTCTAAAGCCAATTGGCGTTCGTGTTCTAACTTTTCTTTAGTACAGAAACAACGGTAGGCCAAACCCTTGCCCACAAGTTCATAAGCTAGTTTTTTGTAGGTAGCCAACTTTTGACTTTGGATGTAAGGCCCATAATTACCCGGGTTGTAAATTGATTCATCGGGAATAATTCCTAACCAGCGCAAGTTTTCTAGTTGTGATTCAATTCCCCCTTCCACATTGCGCTCAGTGTCAGTGTCCTCAATCCGGATAATAAACTCGCCGTTAAAGTGTTTTGCTAACAAGAAGTTAAAGATAGCAGTACGCGCACCCCCAACATGAAGGTAACCGGTAGGGGAAGGGGCATAACGGGTTCTAATCTTTTCCATCGCTTAAATGTGGTTTAAGGCTAAGATACAAACGAAACAACTGGGTCGGGGTTAACTCTTGGGCTCTAACCGTTGCCGCCAAGTCCTGTTGTTGTAAAACATTAGTTAACAAGGTGGGTGGGAGAAGCTGCTTGAGGTTATTGACTAACAGCTTGCGGCGCTGGTGAAAACACTGTTTTAAGAACCGGCCAAAATGAGCTTCATAGGCTACTTGGGGTTTCTTTTTTAACAACATTAACACCGAATCCACTTGGGGCTTGGGCTTAAAAGCAACCTTATCAATTTTAAAAACTGATTTAATTTGCAAATGGTATTGGCAAAACACACCAAAGGCACTATAGTTAGGCGAATTTACCGGTGCTACTAACCTTTGACCAAACTCCCACTGGCACACCAGCACAAATTGTTGTAGTTTGGAAGCCAAAAAGTTAGCAATCAAAGGGGAGCTAATACTGTAGGGAATGTTGCCACAAAGCAAAGGTATAGTGTCAGGAAAATACTGATCCAAGTTTTGTTTTAAGGCATCACCAATGGTTAGTTGTTCTTCAGTTAAGATTTCGTTGACCAAGAGGTATTCCGCTAAGCGTTTATCCAACTCTATGCCATGGTATGGTAGTTGTAACTTGAGTAAAGCCTTGGTTAACGCTCCCTTACCCGGCCCCACTTCAATTAAGGCCGTTGGGTTGAGACTCTTAATTAACCGACAGGTTTTGGCAATTACACTCTGGTCAACCGTAAAGTTTTGCCCTAATTTGCGTGAAGGATAAAAACTATTCACGTTCTAACATTTGTTCTAATTTGGAATACGTTAACCGTCTTCTGGTACGCCTTTTAACAATAAAGAGGTGGGTTAGATAAGACTGGAGTAAGGTAAACAAGGCATTTAAGAACCAGTACACACCCACACCAGCGGCACTAAAAGCCGTAATAACAGCAAACACAAAGTAGAAAATTAACTGCATTCGCTTTGTTTTATTGAGCTGTTCAATGCTCTTTTGCGAATGCGCTTTAGCATTCTCATTGCGTTTGCTAGCCCAAATTTGGGGCAACTTTTGCGAGATAAATTGCACTGGTAAAACAATGATTAAGAAGATAATAAAGGTTCACCCGGTACTGGTAAAGTTCGAGAAGATTTCCGTTAACGGTACCTTAGATAAGTCCCAGAAGTTAAACAGGATAATCGCTTTAATTGGTCTTAAAGTCGTGACAATCCGGTAAATAATGAGGAAGATGGGTAAGGTAACAAAGACCTGAACAAAGGAAGCAGAAGACTTAATGTTGTGCTTCTTGTACAAGGACATGATCTCCATCTGGCGGTTGCGTTTGCTCTGTAAGTCCAACGCCCCTTTGTACTTGGCATTAATTTCTGCTAATTTGCCCTGGACCTCGTTCATTTTTTCTAACGCCAGCGTGGAGTTGAGGGTAATCACTATGGTAATTAACCGTACTAGGAACAACAACACAATCAAAGACAAGATCATGTTGAAGCCCAATTCCGGACCGCTACCTAGAGGAACCCTGGTAGCGTACATAATCGGCAACACTATTTGCGATGCTGGTCAGACAAACCAACCATAAAAGGGACCATACGCTAAGGTATAGTCACTAAAGGTAAAGTAGGGCCCCACATTATTGGACTGCAAGTCATACCGGTAGTCCCCCGTAGTACCAAAGTTATAACCAATTTCTAACCCCGAACCTAACAGTTGGTTGGTTCCCGTTCAGGGCTGTGCTAGGGTTTGGGTACAACCCCATAAACCTACTATGGTTAAAAAGATAAAGATCGCTACTTTAAGGACTTTAAAGATAATGCCCCAGGCCTTTTTGAGATTTTTGAGGCCGTTCTTTTCGTTAACAACGGCCGCTGATCAAAAAGGGTTAAAGGTGGTTTTGAGCTCTTTATGCTTCTTATTTAGGTTTAGGGGCATCGAGTTCTTGGAGCTTTTGGCAAAGGTTTAAAAAGATCGTTTGCTTTTCTTTAAATGTTAATTCAAGGAAGCCTTGATTAATAATAACCAAAACATCAACAGCGTTTAAATTGCAAAACTGTTCCCGCATGATCGCTTTAACTTGCCGTCGAATTAAGTTGCGCTCAACCGCTAACTTGTATTTGGTCTTTGATATCGAAACCGCTGCCCTTCAAGTGCGGACGCGGTTCGGCATAAAGTAGGCCATCAAAAAGGTCGAGAAGAAGCGTCTTTTACTCCTTAAAAGGGCAGCAAATACTTTACGATCACGGAGGTGGTGCTTGGTCTTAGCGCTCACTGGATACGGTGAGTTGGGCGCGTTGTTTTTTTCTTCTTAACTTCAATACTTTACGACCAGAAGCGGTGGCCATGCGAGCTAAAAAGCCATGGGTTTTTGCTCGCTTTAATTTACTTGGTTGATATGTACGTTTCATGATACAGCGCTAAGTATTATAACTAAGATAAAGGACGCACCAATTCGTTAGACACTATCTTACCATCGGAGATCTTAATGATCCTGTTAGCGATCCGCGTTAACGTTTCATCGTGGGTCACCATTAGAATAGTTTGCTTATATTCCTTATTGATTGCTTGTAATAAGGCAATAATGTTTTCGGCTGAATTACTGTCTAAAGCCCCCGTAGGTTCGTCACAGAGCAACAAGCGCGGTTCTTTAATAATGGCACGGGCAATCGCCACCCGTTGCTTTTGACCCCCCGACATCTCGTACGGAAACTTGTACAAGATTTCCTTAATGTCGAGTTTAGCCGCTAACTCCTCGATGTCTAAGTGGCGTTGTTCTTTAGGAATTAGGTTTTGCGAGATGGCAATGTTGTCATACGCACTTAAGAGTTCTATGAGGTTATAGCGTTGGTACACATAACCCAGGTTTAACTTGCGGTAAGTTAAGAGCTGTTCGGTGGTACATTTTTCCAAGGGACAATTGGCCACCACACAGCTGCCCAAAGAAATTGAGTCATAACCGCCTATTAGGTTTAAAAGGGTAGTTTTCCCCGAACCTGATGGACCTAAAATAATCACAATTTCCCCATAACCGATTTCTAGAGAAACATCGGATAAGATAGCAATCTGTTCCTTGGAGTCCTCCCCGTGAACGTGCTTTCACATCTTGTCAATCGAGATCACCATCCCTTGTTTTTGCTCGACATAAGGAGTGCGGTAGGGGGCTTCCAGTTTTTCCTTTCACTGGGAGATCAACCCACCCTGCTTTTTCGGTTTTTTGGGTTTAGGTGCCTTGGTGGTCTTCGTGGGTTTAACTTGTTCCGGTTTAACTGCTTTCGCCTTCGGTTCCTTAACTTTTTTCACCTTAGCGGGTTTGGCCTTCTTTGTTTTCTTGTCTGATGCTAAATAATACTCCAAGGTGGCCTTAAAGCTGTTGGCCTTAACGTTTTCTAATATATCAACACACTCGTCCCAGACAAGTTCTTTCTCGGTTTTCTTTTCTGTATCTTTCATCCTAGAAGCCCTCCTTTAGTTCCGCTGAACGCAGTTTAATTCAGAAGTAAGAACCTATTACAAAGATGGTAGCACCAAACAGGACTATCCCCACCATGTAAGGTAAGAATTCAAACACGTTGGTTAAAAAGACCTGGGCAACCCCAAAGACTAGGGCTTGTACTCCTACTAGTAACCCAGCTAAGATAGCAATCGAGATTAATAACGAAAGTACAAAAGCGGGAATAAAGAAGCACAAGAGACTAACTAAGTTTTGACTATCTTTGTAACCAATCGATTTTAAGGAAATAAAGATCCGTTTCATTTCCTCTAACATCGAGATCCCCAAGACCACACAGGTAATAAAGATAAAGGGAATAATGGTGCCCAACAAGAACCCATCAACTTGGTTCACGGTATTAAACAACACCTCAGCATTATTCCTGATAATTTGAGCACTAGCACCAGCGGCATCTAATTTGAGAGCTGGCACCATGATGTTATCGCCAAAGATACCTCTAGCACCACTAGCTAATGATTGAGTTGAAGATCATTCGCTCGTATATTTTTCGATAACCTTTTTTCTGAGGTTTTCATAGGTCATGCTATCCACACCATTCTCACCCTTTTCCTTAGCCAACTGCTTTAAGATATCCGGATCACTTGGTAAGATTGAGGTAATAATCTTGTTTTCCTTACCCTTAACGAGGTTAGTAAAGTTATTACCAAAATCCGTAAAGTTACCCCAGACACCAATGTTGGAGTTAAGTACTAGCGCTTTACTTACCAAAGAAGGGTTGAGTTCCTTGGAGAAGACCCCGTTAAAGGGAACATAACCAGTTGGCTTGACGTACTCAATCTTGTACTTGTCTTTGGTGGTAGAAGAGGTACTTCCACCCCCACAAGAGCCACCGCCAGACGAAGTACCACCGCTATCATCGGTGGATTGGCCGTCCTTCACCACATCCTTAATGACCCCGCGGCCATCAAAGCCATTGAGCTTTAACAAGCGGTTGGCAATGTTTTGGTTAATATACAACTCTTGGCCAAAGGCATCGGTAGATACCCCGACAACCCGGAACTTCACGGTTGGTGTTTTAGGTGGGCTCTTTAAGAACTTCTCTGAATAGCGGGTCGCAGTGTTCTTGGGTTGAATAAAGATGAAGTCCCCGATCTTGGTGTTATTAGCGTACTGAAAGCCAGCGTTGACAATGACATTCATCTCACTGTCAGAGCTAATTAAGCTCCTCAAGGAGTTACCACCGCTGTCAGTGAGGTTGACAAACTTGGAATCGGGACTAATTCCCGTTAAGGTGTAAGGGGAATCAGGATTCAACTCGGTTTCCTTAAAACCAAATGGTCCTTGAACATAGGTGTATGTCTCCTCTATTTGTGGATCAACCGGGACAATCCCGTACGTTAACTTCGAGTCTTGGTAGGACAACTCCGGATCACCATAGACCTTAGTAAGGAATTTAAGGAATTGTTCGTTAAATTTAACTCCACCAGGATTGAAGACGTTAATCTGGGTTAATACCCTAGCGGCATCCAACTGAATAAAGTTAGTCCGAGGGTTGCGGGTTAAGAAACCATTATTGTTGAGGTTATTAAAGTAGTCATCAGTAAGAACCGCAATTAAAAACTGGTCACTGGAAGCCGATAACAATTGGGTAATTTGTTTAGGAATGGACTTCTCGACAATTTCCCACGGGTTAATGTTGGCACCACCAACGCGAATTGGGAAGTCAATGAGCCACTTCGACAACGAAGCATGACGGAAGAAGTTTTCGTCAGTGTTTAATTGTTGGGCACCACCAAAACTTGGGAGAAGGAGGTTACCGAGTTCGATTTCCTTACCATTATGCTTGATTGGGTTTCCGCCCCTATCACTAATCTTTAACTCTTTGAGGTTGTATGGGTGTTCTTTGTCGTTGCCTTGCTGTTGTTGCTGCTGTTGCTGTTGCTGAATTTGATCACCCTTATCTTTATACAAGGCCTTAATCCCCAAACTATCATCAGTTTGACCAAACCGGGAGTAGGGTTGGATTGCATACCAGCCGGATTGTTCTGTGGGGGTTTGCAAGTTCAACCTATAGTTAAAGTTGCGGTTTAAAACGGTGTTGGACTGCGCTGCCCCAAACTTTTGCGGGATGGTACCGGCAATCCCAATTAAAAGCAGGGCAATCGAACTTAACCCCACATAGGTAAATAACCGCGATAACCGGGAAATAATCAAGGACACCCGGAATTTACCCAAGGGAGTCATGGTGTAACTCTTGCGCTTTAATAAGTGCAAGAAGACCGAGAACTTGGTTTCATTACCCTGGTCAATCAGGACATTAACCGGTTTACTAAAGAGCTGTTTAAAGGCTATTCAGCTAATAAACTGGAAGATCACAAAGATCAACAGCATTGATCCAAAGAAGGAAATCCAGTTAAAGCTGTTGGCCGGTAGGGCTATAAACCAATACCTGGAGATAATCCCCTTAACTTGTCTTTCTAAAAGGAAGGCAAATAAGACCCCAAAGAAGGATGAGGCCAAGGCTACTATGCCGGCAAACACACTCATGCCGGCAATAAACTTGGCTGTGGAGAACCCACCAGCGCGGATAATTGAGAACTCCGTTTGGTTCTTCTTAATAAAAGACTTAATTAAGAGAATGGTGAGGTATAAGGCCAAGATGGATAAGAAGGCCGCAATGACCACACTGGCAATGGCTAAATAGTTCTGCACTAAGTCCGGGAAATAGTTCCGGGCTGTAGTTAAGTACTGGAACTTGGAGATGTCCTTAACGTTAAAGGCGAGATCACTGTCAGGGACGTTGAGATAGCCCTTTAACTTAGAGGCCACCACCTGGATCGGATCTTGGCCAGGGAACTTCGCTTTAATGTCATCCGACTTAAAGGCGTAGTAGTTCTCCTCACTAGCAGCAGGCACAGCAAACAAGACACTGCGGTATCCCTGCTCATTGAGAAAGATTAAGCCTTCATCCTGGGAGTTGGGCAGCGGTGAGTTTGTGGACAATACCGGATAACCGGTTTCTACCGACTCCCCAACCCCTAGAATGGCAAAGGTTAACCCGTTAATTGAGAACTTGTATTTGTCCGGTAAGTCATTTAACCAGTTGGGTAAATTCTTCGGGTTTTCTAACGCTTCTTTTCAGGAGTATTCCCCTTCTAAAGGTAATACTTCCTTGTCGTGTTTAAATAGCCATTGCTGGGATACCACCGCACCGTGGGCTTCCGGGGTTTGGACTACCAAGGACTGTTGGTCGGTGGGTAGTGTTAACGGGTTAACCCCGTTTTTAAAGATAATCGGGATATCTAACTTTTGGCGGTTAGTTACTACCAGTTCATTCGGATCTTGAAACTCCGGGGTAAAGTAACTCAATAAAGCAACATAGTTGTTAGCGGCTTTACTCTTACCTGTATCATCTAAAGCCTCAAAGCCTTTTTTATCTAATGTAGACCACTGCTTAATGGCATTGGTTAAGGTATCAGGAATACTGGAAGCCTCGGGGAGTTGGGCTTGGGCTAAGATGTTAGCTAGTTTGGGGTAGATTTGGAATTTGGTAGAAGTACTCTTAGTATCACCAGTTGAAGAGGAGCTGGAGGAAGAAGATGCATCTGCTAACTGGGTTAAAGAGTCCGTTGTGCCTTGCGTAGTTTTGGCTGCTGCTTTTTTCTTTTCATCTGTTTGAGTAGGCTTAAACTCATACTCAACCTTTTCCAAGAAGCCTTCACTGTTACTCTTGCCCCCATATACCATGTCTAAAGGATTGCTTTTTTCCTTGGCAAGCCAAGACTTAAAGTTAATCCCTTCAATAATAGAAAGGTCGTTGTACTTCTGGGAATCCTTATTGGAGACCAAGAAGGAAGCGGATTCTTCGGAGCTAACATTGGTAGCAATCGCTAAGTTTTGCCGGGAAAACTCCAATTCCTTTAAGGCTTTCGCCTTTAAGAAGGCATCGCGGTATTTTTCTAATAACGCGGTGACCTTCTTCACCAGGTCTTCCTGGAAGATGCTTTTTCATTGGTGTAAAAGGGCAGCATAAACGTTATTTAAATTCTTTTGCGGAGTGTGGTTTTCGGTGCCAATTTTGTCGAATTCAGTTTGGACATCACTAAAAGTTGGATCTTTTACACCGTCATCTGCACTACTTTCCTGCTTATCTTGTTGTTGCTGAGCCAACGCAACGTTCGTTTGTTGTAATTGCGGCTTGAAAGAAACAGAAGAGCGAAGTGCAATCAAGCTAGAGGGATTGCTCTGAGTGCCAGACTTAGCATCGCCTGATTGTGTACTAAAAATCGTATTCTTATCACTATTACTAAACCCCGGTAAAGAATCTAAGATCGTTTCCTTTTCGGCTTTAACCTTATTTTTGAGCCAGTCCTTATTGATGTTAAGCTCAAACGATTTGAGGCTATTAATCTTCTTCTTGAGGTTATAGTCACCAAAGGAAGAACCGCTAGAAATAGTTTCTGCTTGCTTTTCTAATCAGTCAAATTCTTCCTTATCTAAATGAGATAACTTACTTGTATCTAAAGTAAAAGTTAAGGAGAAAGATTGTTTTTCTTTTTCCTCTTTTTCTTTTTGGAAGACCTCTATTATTTCTTTAAAGGGATTAGTCTCTAACCAAAACTGGGAACCCGGACTAATCGGGAGCACAATAGGTACATTAGCGTACAGATTGGGAAACCAGTTAGCAGCTTCTTGCCCTCCGGTAAATAGATAAGAGGTACTTAGTTTACTGGTTTCGCTTTCTCCACTGCTACTACCAGCTTGAGTTTCATCACTTTTAAACCAAACTTTGTCTTTATTATTGGTAGTGTATTGTTCTTCTACATTACTATCTTTTTTGGGCTCCTCGTTATTTGTGGTTTCTTTAGCATCAGTCATCTTGGCCTGAGCTGCTAACGGTTGTAAAACTGTTGTGGCACTACTCGCTTGCTTGATTTCTGATTTTTTTTTAGTAGTTTTCTTATCCTCACCCTTCCCTTTATCACCAGGAAAGCGAGCAATCCCAGCGCGCAGGGGAATCCCATCCAGAATTAAACCATCGACAAAGTAACCTAAAAGGTTACCTTCTTTGTCATAAATCAGGTTTTGGGGATCGGAAACCGTATTTTGGTTTAAGTTATTACTTTCTTGATCGGTTCCAGTAAAAGAATAGGTATTTTGAAAGGAAACAGCTAAACCCTTATAGATCCGTTTTTCGGCAATGTTGGCTTCCCCGTAAGCTTTCAAAATGCCTCTTTTACGAGCTAAAAAGAGTTTGTCTTTATCGTTAACATCACCTTTTCGTGGGTTATTATCTGATGATTTGGTGGTTGAATCTTTCTTTTCAGAATCACTCCCAAAATCAGATTCTTGGTAGGGCCAGATAAAGTTAATCTTCTTTTCGGTTAACTTGATTTTGGAAAAATGTCTTTTTTCTTCTTTAAGTTCCGTTTTATACACCGGACTACTTTCTTTAAAGATAGCCGGTGCTACTATGTCAGAAGAATTCGTTTTTTGAACAAGGGCAGTATAAGTATTAACAAGGTTATTACTTAACTGAAGAATCGACGTTTCCAGGAAGATAATGCTGAAAAGGACGAAAATAATCCCAAAAAGTAAAAAGAAGAACTTTTTGAGTGACTTAAAGATCTGTTTAAAAAACGAAAACATTTAGTTTAAACTCAGTTAATTTCGTTAATAGTCTTGGGTCTTTGGTTAAGATAATCAGCCACAATCTTGCCATCGTGGATCTTAATGACCCGCTTAGCTAGCTCCACAATCTTTTCATTGTGGGTAACTAATACTATAGTAGTGCCCTTATCGCGGTTATACTCCACAAAGAACTGGAGGATCTTTTTGGAAATATCCACGTTAACAGCACCAGTTGGCTCATCACCAAATAAAATACGCGGCTCTTTGATTAGTGCCCGCGCAATTGCTACCCGTTGTTGTTGTCCCCCAGAGAGCTTGGTAATCTTTTTATTACGGTGCTCTTTGAGCTCCAACCGCTCTAACAGCTCTTCCAAATTGTTGTTATGGCGCTTTTTAAAGGGAACTGCCAGTTTAATGTTGTCATCAACGTTCAGATCCCTTAAAAGGCCATACTGTTGGAATATATAACCTACGTTTTTATTCCGTAGTGATGTTAATTGGGCATCATTACAACAAATAGTACTCCTACCACACACAAAACTCACCCCGCTAGTAGGTCGGTCTAGGGCGGAGATTAAAGAGAGTAAACTAGTCTTACCAGAACCAGATTTACCCAAAATAACCACAAACTCCCCAGGGGCAATTTTGAGATCAATGTGATCACAGATTAACTGGTTTGTGATCCCATTGGTCACTGCCTTACAAACGCCCTTGAACAAAACATCGTAATGTTTAGCCTCAGGGTCTTTGTAGTGCCGTCTTTTAGGGTGCTTCTTATTTAACTTAACGATTAGCTTTGACGCTTTTTTACTTTGACAAAAATCGGGTCATTGCAAACAATAATCGAGGCTTCGTTTATTTTTAGCATTTAAAGACATTGAAACATGTCAGGTTTAACAACGGGACAGGCCAGTTCAATTAAACACTTAAAGAATTATAAAAGGACTAAATTACCAAGGAAGTGATAATGTCTTGGAGGGAGTCGTCCTTCTCAATCATTTTAGCAACCCTTTTAACTGCAGCCACCACTGTAGAGTGGTTCCTTTTAAAAATTTTGCCTATTTGTTGGAACTGCATCTTGTACTTTTCCCTTAAAATATAGTTACAAACGTCACGAACACGAACTAACTCTGCTTTACGGTTCTCAGACAAAACACTGTCCACCGGTACATTAAAGCGGCGGCAAACGTTTTCTATTAATAAATAAGGATCAAAGCTCTTTTTGTGGAACTTCTCAAATTCCTCAAACAGGATTTCCTTTAGATTGTCGTTGTTAATCAGGTTTTGCTTAGTTGTTTTGACATAAAACAGCAACTTAGTCGCAATCCCGTTTAAAGAACGAACATCGTTACCAGATATTTGCGCTGCCTTTTCGCGTGCTTCATTGGTAATTTGGATGTTGGGATCCTTTTCTTTGAGCTTAACGGCCAAAATTTCACACAGGGAGCTCAAATTGTGTTTTTCAATCTTTAACAAGAGTCCCGATTTAAAGCGTGATACCATCCGTGGATCGATGTTGGCCAACTCATCGGGGGTTTTATCGGACACAAACACAATTTGCTTGCCCTTACTTACTAAGTTATTAAAGATGGAAAAGAGGATCTCTAAGGTTTTTTCCTTTTTCCCGAAGATCTGAGTATCGTCAATTAAGAGTAAATCCAATGACTCGTAAGCAGCCTTGAGCTTTTCAATGCCTTGATCCTTTTCCTTTTGGTAAAAGGCATTGACAACCTCCTGGGCAAAGTCACTCGATACCACATACTTTACCTTAGCGTTGGGAAAGTGGAAGAACTTATCATTCCCAATCGCTTGGAGTAAGTGGGTTTTACCCAGACCAGTCTCCCCATAGATAAATAAGGGGGAGAAATCAGCGTCCTGAGTTTCGCTAATCCGTACACCCGCTTCATAAACCCGGCGGTTGCCATCACCAACCACAAAGTTTTTAAAGGTAAAGTTTTTGCTTAGCCCCGAACTTTGGTAAAGCGTTTCGCGGTTATCTTCCTCTAGTTTGGCTAAATTGATAAAGAAGTCCTGTTCGTTGACAAACCGAACTGCTCTAATGTCATCGTACAGTTTGCTAGCTAACCCGACAATATCAGGGTTGCTATTGAGGGTTTCACGGGCAAACTCATTGTCCACAATCACAAAAAGGACATTATTTTTTAAAGCAAACCGTTTAAGATTCTTAATGTACTTGTCATAAAAACCTAAAGTAGTTTCGTATTGCTTTTTTAAAAGAAGTTTAAAAGCACTAAATTGTTCCATTACTCTTCTATTACCTGATCAAGAATCATCGTTAAGATCCGCGAAATCGGAATGTTAACCGCTTTAGCATAACGCTTAACCTTATTCCAAACACGGGTACTCGTATAAAAGTGCACCCCGGTGGTTAAGCGTTCTTGCCGTTCTAAGGGAATAATACCGGGCACTTTCGCCTTTGGTGGTGGTAGATTTTCTTCTTCCTGCAATTTATTAGATGGGCGAGTAGTCTGCTTAACTTGTGGCTGTTCAACCGCTGGTTGTGGTTCTGGTTGGGGAACAGGTTCCACAATTGGTTGCGTAGCCTCCACCTGAATATTAGATGTAGCTACTGGTTGTTGATTAAAGTTAGTGGTTGTAGGTTGTTGAAACTCATTTAAGTGCACTTCTGCAGTTTGGTGAACTGGTTGGGTTGGAACCTGATTATTAAAGTTAGTTGGTTCTGATTGCGGTTGCACATTAACTGGTTGGGTTGAAGTTTGTGGTTCTTGTTTGGGAAACTGGTTAAAGGATTTCACAAACTCATCGCCAAACTTGAGTAATGGTCCAAAATCAAACTGGGGCATGTCCTTAGAAAAGAACTTTCTGTCTTGACTAGATTGATTTGCTTTAGTTTGGTTAGTTAGATCATGTTGGGGTTGGACAACCGGTTCTAGTTTTAATTGTTGTTCTACCTTTTTAACTTCTTGAACAGTTTCTGCATCATCTTCATCCTGAGCTAGGTCAAAGAAGAAGCGTTTCTTTTTACTGATGGCCATGGTTGACTAGGTTAAGAATTTCTTTAGTAATCTCTAAATATTCAGTTTGGTACTTCTTGTTGGATGTTGGTGATACCAAAGAGATAGGTAACTTTTCATAACCAACAGCAGCAGATGACTTAGATGTTAAAGATACAAAGTGTTCTGAAAAAGCAACGTTGTTCTTGTGCGCTTTTGATTTCACCAACTCAATCACATCATTGTGTAATCTGGTGCGCATATTGACCTTGGTTGGTACCAAAATGGTTTTTAAGTTGGGGTTCTTTTCCTTAAAAGTATCAATCGTTTCGACAATCCGCATTAAACCCAACATAGAGTATTGATCAGGTTCAAACGGGATCACAATCACATCAGAGAGGCTCATGGCTGTGGAGACCAAGGTAGCCATGTTAGGCGGTGTGTCTAGTAAAACAAAGTCATACCTGCGGGTGAGTTGTTTAACTATTTCCGCAATATCAGAAGCCTTGTACTTTTTGCGAGCAATATCAATGTCCGCAAAATTTAACTCAAAGTTACAAGGCAAAATGTCTAGTCCTTCATAAACGGGCAACAAACAGTCATCAATTTCAATTGAACTGTTGGCGCCGTTAAACTTAGGCACTTTTAACAAAATATCAATTAAGGTGTTATTTAATCTTTCTGGATTTTGTCCAAAAGAGGCTGAAACGTTGCCCTGACCATCAAGGTCTAAAATAACTTTCCGTTGTTCAGGGCACAACTTTACAAGCGAACCAGCAACGTTAGTGGCCATGGTTGTTTTTAAAACGCCACCTTTGTTATTAACAAAAGAAATAATCATATGCGCGCGTATAAGTTTGCTTAAATTCTAATATTTATTTAGTATTATTCCGGTAT", "length": 816435, "complete": true, "type": "contig", "topology": "circular", "simple_id": "contig_1", "orig_id": "GCA_002090215.1|CP020689.1", "orig_description": "HMT-732 Mycoplasmoides pneumoniae S63-tet-R" } ], "run": { "start": "2026-01-31 09:15:51", "end": "2026-01-31 09:23:08", "duration": "7.27 min" }, "version": { "bakta": "1.11.4", "db": { "version": "6.0", "type": "full" } } }