{ "genome": { "genus": "Absconditicoccaceae [g1]", "species": "bacterium hmt-345", "strain": "HOT-345", "taxon": "Absconditicoccaceae [g1] bacterium hmt-345 HOT-345", "complete": false, "gram": "?", "translation_table": 11 }, "stats": { "size": 1144105, "gc": 0.3820514725484112, "n_ratio": 0.0, "n50": 863952, "n90": 169615, "coding_ratio": 0.6530764221815305 }, "features": [ { "type": "cds", "sequence": "contig_1", "start": 46, "stop": 450, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF05345.18" ], "nt": "GTGGATGAGATTCCGCCAGTGATTAGTCCTCTTACTGGAGTGAGCTATCAACGTTCTACGCCTATTGTGCCTATTCCATTGGTAGCAACTGATAATAGTGGATCGGTAACGCTTCAGGTAAACGGATTACCAGCAGGATTGACCTTTGATATGACGGGTTCTCAAATTATTGGAACGACTCCAGCTACTCCAGGAAGCTATCCTATCCAAGTGGTTGCGATTGATCAGGCAGGAAACCAGACTACCGTTCAGTTTACACTTACTGTGATAGCTCCTCCAGCTCCTAGTAGTAGTTCAAGTTCCTCTTACCGTTCTGGTGGAGCAGGAACAAGCTTACCAAAAGATAATTGTCCTAACGGTGATTTCTCATCAAGCTATTATGATGGTAGCTGTGGAAATGAGTGA", "aa": "MDEIPPVISPLTGVSYQRSTPIVPIPLVATDNSGSVTLQVNGLPAGLTFDMTGSQIIGTTPATPGSYPIQVVAIDQAGNQTTVQFTLTVIAPPAPSSSSSSSYRSGGAGTSLPKDNCPNGDFSSSYYDGSCGNE", "aa_hexdigest": "402a31a051651b46b4d51484f0edf0c7", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF05345.18", "name": "He_PIG", "length": 89, "aa_cov": 0.6119402985074627, "hmm_cov": 0.9887640449438202, "evalue": 4.969192993379241e-14, "score": 49.02809143066406, "start": 8, "stop": 89 } ], "seq_stats": { "molecular_weight": 13612.885599999985, "isoelectric_point": 4.0500284194946286 }, "id": "NKOCPLAKPK_50", "locus": "GCA003260355_00001" }, { "type": "cds", "sequence": "contig_1", "start": 556, "stop": 1014, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAGAACTCTTGAGACAAGCTCATCAGATGCTCTATAGTTATCAGCTTACAAGATGGCAAGGCACCAAAGACTTTGCTCCAGAGAGAAGCTTAACCAGACAAGAAGCAGCAAGATTTATGACTGAATTTGCTACAAATGTGCTTTGTAGGAAACCAAGTAGAAACTATGCACATCAGTTCATAGACTTAGGAGATGCTGATCCAACGCTTCTACCTTATATCTATAAATCGTATGATTATCTTATCTTTAATGGGGATGGGAATCCAAACGGAGATAAAGCACAAACGACGTTTAGACCTTATGATCTGATAACGGTAGATGAACTCTCTGCACTTCTCACCAGACTCGTGAAGAATCAAACCATGGAAGAGCCAGCAGAAGATCGAGCAAGAAATTATAGGAATTATATTAGTTCTATCGCCTCAAACTCAGCTTTAAAGAATGATATTAGATGA", "aa": "MTELLRQAHQMLYSYQLTRWQGTKDFAPERSLTRQEAARFMTEFATNVLCRKPSRNYAHQFIDLGDADPTLLPYIYKSYDYLIFNGDGNPNGDKAQTTFRPYDLITVDELSALLTRLVKNQTMEEPAEDRARNYRNYISSIASNSALKNDIR", "aa_hexdigest": "20b5e1bdb9b627a2d1bfcbd6fa6b8696", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17671.622299999988, "isoelectric_point": 6.733056068420409 }, "id": "NKOCPLAKPK_51", "locus": "GCA003260355_00002" }, { "type": "cds", "sequence": "contig_1", "start": 1211, "stop": 4018, "strand": "-", "frame": 3, "gene": null, "product": "BIG2 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EFK5", "UniRef:UniRef90_A0A328EFK5" ], "nt": "ATGAAAACAAAGAGAAAAAAATTGATCACTGTGATGATAGGTATCATCCTTTTGATCAGCCAGCAAATCATTGCCGGTACGAACTACACCAAGACCGTAACTTCAACAGGAGGAACGGTAAGTGCTACAGAGTTTGATGCAACACGCATAATCGCTGGAGGTATCGCTAAATGGAACAACCTCCGCATCAAAAACGGAGCAACCGTAACGATAGATTTTAGTGGGATTCCTGATATTCAGTCAGTAGAAGGTTCGAGCATCACAACGGCTCAAATCACCAGTGCCGGCACAGTTGAGATAAAGTTCAAAACAGGTACAGGAGCATCTGAAAAAGAATTCAGGATCAATCTCCATGTAAACTACTATGGCGTTCCTCAGTTCAATGGAGCAGACGTCTCGAGTAATATCGTGATCACGGAATCCAATAAGAACAATGTCGTGACAATCCCTGGCGGTTTTCCTAGCATTACATGGTATAGTAATGGATCTGTATTATCTAGTCTGAATGACGAATCATCAGTCTCTCTGTCAGATTTACAAGCAACTGGGAAAACCAGTTTTACTGTCAAAATGAGCGATGGCACATTGAATGACTTGCCCGATGACACACCAGGAGGATTAACTCAATACTCATTCAGTATCACTCCTCTTGCTCCGACTCCGACGCTTTCAGTAGCTCCTGCATCTATTACTAATGTAGCTGCTGCTGGAGAATCGAAAACACTGACTATCACTTCTAACGCGGCATGGACTGCCACTTCTGATCAGTCATGGGCTACACTCTCTACTTCTAGTGGAACGAACAATGGAACTGTAAATGTTACACTTGCTCAGAACACTGGAACTTCTCCGAGAACAGTCACGCTGACCTTTACTGCTGGTTCTTTGACGAGGACGGTAACGATCACGCAGGATGGAGCTACTCCGACACTGTCAGTGAGTCCTACAACACTCTCACCCACAGCTGCTGGAGGAACAGCTACGGTAACCGTAGCTTCCAATGTAGCAGTATGGTCTGTTGCTTCCAATCAAGGATGGGCTACGGTCTCTCCTGCGAGCGGAGCAAACAACGGAACAGTGACAGTTACATTCACAGCGAACACCGGCACTTCCCCGAGGACAGCAAAGCTGACCTTCAGTGCGACCGGACTTCCCAATCAAGAGGTTCAAATCACACAACCGGGAACTGCACCGATGCTTTCAGTAGCTCCTGCATCTATTACTAATGTAGCTGCTGCTGGAGAATCGAAAACACTGACTGTCACTTCTAACTTGGCATGGACTGCCACTTCTGATCAGTCATGGGCTACGCTCTCTACTTCTAGTGGAACGAACAATGGAACTGTAAATGTTACACTTGCTCAGAACACTGGAATTGCTCCGAGAACGGTCACGCTGACCTTTACTGCTGGTTCTTTGACCAGAACGGTAACGATCACTCAAGATGGAGCTACTCCGACACTGTCAGTGAGTCCTACAACACTCTCACCCACAGCTGCTGGAGGAACAGCTACGGTAACCGTAGCTTCCAATATAGCAGTATGGTCTGTTGCTTCCAATCAAGGATGGGCTACGGTCTCTCCTGCGAGCGGAGCAAACAACGGAACAGTGACAGTTACATTCACAGCGAACACCGGCACTTCCCCGAGGACAGCAAAACTGACCTTCAGTGCGACCGGACTTCCCAATCAAGAAGTTCAAATCACACAACCGGGAACTGCACCGATGCTTTCAGTAGCTCCTGCATCTATTACTAATGTAGCTGCTGCTGGAGAATCGAAAACACTGACTGTCACTTCTAACTTGGCATGGACTGCCACTTCTGATCAGTCATGGGCTACGCTCTCTACTTCTAGTGGAACGAACAATGGAACTGTAAATGTTACACTTGCTCAGAACACTGGAATTGCTCCGAGAACGGTCACGCTGACCTTTACTGCTGGTTCTTTGACCAGAACGGTAACGATCACTCAAGATGGAGCTACTCCGACACCGCCGACACCTCCAACTCCTCCAACTCCTCCAACTCCCCCGACTCCCCCGACTCCTTCTACTCGAACACTCAGCCTGAGTAGAAGAGCAATCAGAAGAGACAGAAAAGCGGGAAGAGAGGAGATCCGAGTATCCTCAGATCAGGATTGGTCTATTATCAGTGCTGATTCTTGGATAACCTCTATGACAACCTCGGGAAGTGGAGACGAAGTAGTAACTCTCAATATTGAGGAGAATACCACAGGGAGAGAAAGGGTAGGACAAGTAAGGTTTACCTCAGGTTCTCTGAGCCAAACGGTAACTATTTACCAAGCTGCCACCACAGATCAACCTGGACATAACCAAAAAATTGAAAAGCTGATCCTTAGCGATACTTTAGTTACGCTGAATGGAGTCGCCTCCTATCAACTTAGTGTTGATATTCTACCGACTAATGCTACCTCTAAGGATATAGTATGGAAATCTAGTGATCCTACGATTGCCACGGTTGTGAATGGTCATGTAACTATCCTCAAAAAAGGAGAAGTTACGATCACTGTCAAGGCACTTGATGGAAGCGGAGTTTCTGCCAGCTGCAAGATTGATATTCAGTCTACAGTAGGCAATGAGCTCTATCCAGAAGAGCCGAGCATATGGAGCAATGGCAACATACTCCATATTAGACTGGTAAAACCTGAACAAGTTTCAGTCTATACCATAAGCGGGCAACAAATTCGTCATCAGAAGATGAATGGCGACAATGCAGTATCTTTACAACAGGGTATCTACGTTGTAAGGATCGGCGACCAAGAAACGAAAAAGGTCGCTATCTACTAG", "aa": "MKTKRKKLITVMIGIILLISQQIIAGTNYTKTVTSTGGTVSATEFDATRIIAGGIAKWNNLRIKNGATVTIDFSGIPDIQSVEGSSITTAQITSAGTVEIKFKTGTGASEKEFRINLHVNYYGVPQFNGADVSSNIVITESNKNNVVTIPGGFPSITWYSNGSVLSSLNDESSVSLSDLQATGKTSFTVKMSDGTLNDLPDDTPGGLTQYSFSITPLAPTPTLSVAPASITNVAAAGESKTLTITSNAAWTATSDQSWATLSTSSGTNNGTVNVTLAQNTGTSPRTVTLTFTAGSLTRTVTITQDGATPTLSVSPTTLSPTAAGGTATVTVASNVAVWSVASNQGWATVSPASGANNGTVTVTFTANTGTSPRTAKLTFSATGLPNQEVQITQPGTAPMLSVAPASITNVAAAGESKTLTVTSNLAWTATSDQSWATLSTSSGTNNGTVNVTLAQNTGIAPRTVTLTFTAGSLTRTVTITQDGATPTLSVSPTTLSPTAAGGTATVTVASNIAVWSVASNQGWATVSPASGANNGTVTVTFTANTGTSPRTAKLTFSATGLPNQEVQITQPGTAPMLSVAPASITNVAAAGESKTLTVTSNLAWTATSDQSWATLSTSSGTNNGTVNVTLAQNTGIAPRTVTLTFTAGSLTRTVTITQDGATPTPPTPPTPPTPPTPPTPPTPSTRTLSLSRRAIRRDRKAGREEIRVSSDQDWSIISADSWITSMTTSGSGDEVVTLNIEENTTGRERVGQVRFTSGSLSQTVTIYQAATTDQPGHNQKIEKLILSDTLVTLNGVASYQLSVDILPTNATSKDIVWKSSDPTIATVVNGHVTILKKGEVTITVKALDGSGVSASCKIDIQSTVGNELYPEEPSIWSNGNILHIRLVKPEQVSVYTISGQQIRHQKMNGDNAVSLQQGIYVVRIGDQETKKVAIY", "aa_hexdigest": "eab0a31829866a488c7e2d8a8a0d32dd", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A328EFK5", "query_cov": 1.0, "subject_cov": 0.9659090909090909, "identity": 1.0, "score": 1494.0, "evalue": 0.0, "valid": true, "product": "BIG2 domain-containing protein", "uniref50_id": "UniRef50_A0A328EFK5" }, "pscc": { "uniref50_id": "UniRef50_A0A328EFK5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EFK5" ], "product": "BIG2 domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_52", "locus": "GCA003260355_00003" }, { "type": "cds", "sequence": "contig_1", "start": 4541, "stop": 4828, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGACACTCGCTCAGGTCGATGTAAAGTCCAATCTAACCAATGCTGTGCAGAATATTCAGAAGGTCTATTTTACTCCTAATGGGTTGCCTAGTGATGATGAAATCTTGGTAGAAGCGTATGATGGACTTGTGAAGATTAGTGGAAGAGTGCTGCTCCAAAAAGACGGTGATGAAAACCTCCTTACTCCAGAAGCAGGGAAGACTTTTATTATTCAGGGAAAGAAGAATATAGTGTATGGTAAAGATGCTTTTTTGCTTGGTGGTAAGCTGAATAAAGCCTCGACTTGA", "aa": "MTLAQVDVKSNLTNAVQNIQKVYFTPNGLPSDDEILVEAYDGLVKISGRVLLQKDGDENLLTPEAGKTFIIQGKKNIVYGKDAFLLGGKLNKAST", "aa_hexdigest": "9d893bfa41ef88e3998c1a8c7e364d8d", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10295.685099999988, "isoelectric_point": 6.1684175491333 }, "id": "NKOCPLAKPK_53", "locus": "GCA003260355_00004" }, { "type": "cds", "sequence": "contig_1", "start": 5000, "stop": 5389, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTACTGAAATCAATGGAGATGGAAGCTTCGGTGCGGGAAAGAATGTGAAGGCTGCCAGTAATGCTACAGATAGAGTTTTTCTCTGGAGTGATAATTATTCGTCTTGGTATCGTGTTGATGGGAACGAAATGCGTCTTAGACCAGTAAAAGAGGAGACCTTCCTTATTAGAAGTCTGAATGGGATCGGTATTGGGACGAATGATCCTCAGCTCAAGCAAGGTGTAGATGTGAACGGACTTGTCCAGATCGGAGATCAAGTGCTTGCCTGTGGTGGTGATGCTCAAGGAGCTATGAGGTATAGGAATGGATGCTTCTGTAACTGTAATGGTGCTACCTGGGTAGCGATGAACCCTTCTGCTAAATGTCTCAATGTGTGTAGGCAATAA", "aa": "MLTEINGDGSFGAGKNVKAASNATDRVFLWSDNYSSWYRVDGNEMRLRPVKEETFLIRSLNGIGIGTNDPQLKQGVDVNGLVQIGDQVLACGGDAQGAMRYRNGCFCNCNGATWVAMNPSAKCLNVCRQ", "aa_hexdigest": "e349f5287d8e6a855a8530239b4527d1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13983.634899999985, "isoelectric_point": 7.540851783752442 }, "id": "NKOCPLAKPK_54", "locus": "GCA003260355_00005" }, { "type": "cds", "sequence": "contig_1", "start": 5610, "stop": 5870, "strand": "+", "frame": 3, "gene": null, "product": "4'-phosphopantetheinyl transferase domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EFM6", "UniRef:UniRef90_A0A328EFM6" ], "nt": "ATGAGACAAGGACCCTATGGAACGGCGAGGCTCTATAAAGCTTGTCTGCTCCAGCAGAGCTTTGCTTGCTCTGAGGCGCAGCTTCTCCAAGCTATGAAGGAGCACTACTTTGAGGGGCAGTATTTTTCTAGTTCCTATAGTGGAGCATGGCTCTTTTTTGCATTGGCAGAGAGGAAGCTTGGGGTCGATGTAGAGGTGATTAAGTCGAGGTCTGCTCTGCTTTTGGTGGCTATGGAGGCTGAGATGAAGCCTCTTTTCTGA", "aa": "MRQGPYGTARLYKACLLQQSFACSEAQLLQAMKEHYFEGQYFSSSYSGAWLFFALAERKLGVDVEVIKSRSALLLVAMEAEMKPLF", "aa_hexdigest": "6b7ae7dda7033185106edc1b4556a08f", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 5496, "stop": 6107, "observations": { "mutation": [ 5517, 5583, 5868, 6003, 6078 ], "stop": [ 5517, 5583, 5868, 6003, 6078 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EFM6", "query_cov": 1.0, "subject_cov": 0.4236453201970443, "identity": 1.0, "score": 168.0, "evalue": 1.77e-51, "gene_start": 1, "gene_end": 86, "reference_start": 39, "reference_end": 124, "reference_sequence": "MRSFTSSGSEVQFLLEQSPFPPTKEVESFGFSASFLQKMRQGPYGTARLYKACLLQQSFACSEAQLLQAMKEHYFEGQYFSSSYSGAWLFFALAERKLGVDVEVIKSRSALLLVAMEAEMKPLFGKADWKSFYLLWTAKEAILKRWDARSLDLMDQISFFAVEKKPLQLGGVHFDRELKYGFEGQAGMVRSGENGLLAWSFAD" }, "paralog": false, "identity": 0.9753694581280788, "score": 389.0, "evalue": 1.03e-140, "description": "Internal stop codon at 5517, 5583, 5868, 6003, 6078. Nonsense mutation around 5517, 5583, 5868, 6003, 6078.", "psc": { "uniref90_id": "UniRef90_A0A328EFM6", "product": "4'-phosphopantetheinyl transferase domain-containing protein", "uniref50_id": "UniRef50_A0A328EFM6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EFM6", "UniRef:UniRef50_A0A328EFM6" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EFM6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EFM6" ], "product": "4'-phosphopantetheinyl transferase domain-containing protein" } }, "id": "NKOCPLAKPK_55", "locus": "GCA003260355_00006" }, { "type": "cds", "sequence": "contig_1", "start": 6233, "stop": 6565, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGACCAGATACGAAGCTGCACTCTTCTTCTACCGCTTCCAAATCAAACAAAAAATCACTTCCCACCTCAATACCGAATCGCTCAAAAACGAACTCATCTCTACCAAAAAAACCAGCGATGGAAAGTTCGCTAGCGGAGATAACGAAGGAACCTACGCCGTGACCTTTGATATCAACCTCCTCAAAAACCAGTTTTTCCAAGCTGGATTTATCGAACTGCTCGGCACCAGATATGAACTCAAAAAGACCAATATAACTGTCTTTGACATCGGAGAAGAAAGCTTTGTGCGATACGGAGACCTCTTTGATATGCAAAAAGAGACTAAAGTCTGA", "aa": "MTRYEAALFFYRFQIKQKITSHLNTESLKNELISTKKTSDGKFASGDNEGTYAVTFDINLLKNQFFQAGFIELLGTRYELKKTNITVFDIGEESFVRYGDLFDMQKETKV", "aa_hexdigest": "251883a7a86298faa0b94127c5f7a174", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12774.33299999999, "isoelectric_point": 6.583853721618653 }, "id": "NKOCPLAKPK_56", "locus": "GCA003260355_00007" }, { "type": "cds", "sequence": "contig_1", "start": 6605, "stop": 7663, "strand": "-", "frame": 3, "gene": null, "product": "SLH domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MYG3", "UniRef:UniRef90_A0A328EJ00" ], "nt": "ATGCACAAAAATGGACTGACGCAGTATGATACCCTAAGCGGCTACAGACCAGAAGACCCGCTTCTTAGAGAAGAGGCTGCAAAAATCATCGGACAGGCCTACAGCATTTTAGGCTTCCCTCAGGAAACAAAAACTGAGGAATGCAACTTCAAGGACCGCGAACTCTTCAATCCAACGCTAGCTCCGTTTATCACCTCAACCTGTAAAGTAAGCATCTTCAAAGGGAATAAAGGACTTTTCCACCCACATACTCCACTTACCAAGGCTGAAGCTCTAACCGTTCTCATTAGAATCCTAGAAGGCAAATCAAGTAGCGAAAGCTTCGATCCTCGATGGACACTCTACTTTATCAAAGCAAAAGGAATCTCCCTCACCCAAGAATCTCAAGTAATGCAGCTCCAGAGACCTGTCACAAGAGGAGAGATCGCACTACTTATCTATAGATTCAAGGCAATTATTCTCAATCAGCAGCTTCATCAAGCAGCAAAAAATCAGCTTTCAACGATCGATACGAACCCTCAAGCCTTTCTTACTACAACCCAAACAGGCACTCAGCTCCAAACCGGCACACAAACCTCACAAACCCAAAACAATGATATCCTCGGACTCATGAGCGGCAATAATTCAAGCACGACAAGCTCACTCAGCGTGCTCAATTCTCCCGAGGTCAATGAAGCCATCACTCGAATGTATGATCAGAAAATGACCAGCTCAAGGGAAATTTCGACCTACCAACCCTTCGCACAGCTAACGAGGGAACAGGCCGCCAAAATGTTCACCCAGTTTGCAAAAGCACTCAATATCTCTGCCCTCACAGGAGGAGCACTCCCATGTAAATTCAGAGATCTCAATCAGGCAGACAGCTCACTCAAGTCAAGTATCGAAGAAGCATGTAAGCTCGGCATTATGCAGGGAAATAATGGCTACTTTAGCCCACAAACCATACTAAAAAAATCTGAATTCATCACAATGCTTATCAGACTTTCCGAGGGAAAAAGACTCAACGAAAACGGGAACCCTCGATGGAAGGACTACTTCCTCAAAGCAAAAGAGCTCTGA", "aa": "MHKNGLTQYDTLSGYRPEDPLLREEAAKIIGQAYSILGFPQETKTEECNFKDRELFNPTLAPFITSTCKVSIFKGNKGLFHPHTPLTKAEALTVLIRILEGKSSSESFDPRWTLYFIKAKGISLTQESQVMQLQRPVTRGEIALLIYRFKAIILNQQLHQAAKNQLSTIDTNPQAFLTTTQTGTQLQTGTQTSQTQNNDILGLMSGNNSSTTSSLSVLNSPEVNEAITRMYDQKMTSSREISTYQPFAQLTREQAAKMFTQFAKALNISALTGGALPCKFRDLNQADSSLKSSIEEACKLGIMQGNNGYFSPQTILKKSEFITMLIRLSEGKRLNENGNPRWKDYFLKAKEL", "aa_hexdigest": "a478032fee47c811a3c6a87737967757", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 6302, "stop": 7963, "observations": { "mutation": [ 6607, 7696, 7753 ], "stop": [ 6607, 7696, 7753 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EJ00", "query_cov": 1.0, "subject_cov": 0.5632, "identity": 0.997, "score": 684.0, "evalue": 5.89e-244, "gene_start": 1, "gene_end": 352, "reference_start": 107, "reference_end": 458, "reference_sequence": "MKTKIGMLILGLALLNTFPALASDQKIVIDPINKKILVQSGNQTFSSESFSSTGTTISTNSQAPAATNQSESTSQSGTALSQPSQKPSPLNMELTGGTELEKAIFRMHKNGLTQYDTLSGYRPEDPLLREEAAKIIGQAYSILGFPQETKTEECNFKDRELFNPTLAPFITSTCKVSIFKGNKGLFHPHTPLTKAEALTVLIRILEGKSSSESFDPRWTLYFIKAKGVSLTQESQVMQLQRPVTRGEIALLIYRFKAIILNQQLHQAAKNQLSTIDTNPQAFLTTTQTGTQLQTGTQTSQTQNNDILGLMSGNNSSTTSSLSVLNSPEVNEAITRMYDQKMTSSREISTYQPFAQLTREQAAKMFTQFAKALNISALTGGALPCKFRDLNQADSSLKSSIEEACKLGIMQGNNGYFSPQTILKKSEFITMLIRLSEGKRLNENGNPRWKDYFLKAKELGILNNEDALSFELPLTRYEAALFFYRFQIKQKITSHLNTESLKNELISTKKTSDGKFASGDNEGTYAVTFDINLLKNQFFQAGFIELLGTRYELKKTNITVFDIGEESFVRYGDLFDMQKETKVGTTSFVISNGNLVQGTIRLTSPNKTRLIKESKSTNARFTMSSL" }, "paralog": false, "identity": 0.9529837251356239, "score": 1022.0, "evalue": 0.0, "description": "Internal stop codon at 6607, 7696, 7753. Nonsense mutation around 6607, 7696, 7753.", "psc": { "uniref90_id": "UniRef90_A0A328EJ00", "product": "SLH domain-containing protein", "uniref50_id": "UniRef50_A0A660MYG3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EJ00", "UniRef:UniRef50_A0A660MYG3" ] }, "pscc": { "uniref50_id": "UniRef50_A0A660MYG3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MYG3" ], "product": "SLH domain-containing protein" } }, "id": "NKOCPLAKPK_57", "locus": "GCA003260355_00008" }, { "type": "cds", "sequence": "contig_1", "start": 7751, "stop": 7963, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTCATTCTCGGGTTGGCTTTGCTCAACACATTTCCAGCCCTCGCCAGCGATCAAAAAATCGTCATCGATCCTATCAATAAAAAAATCCTCGTCCAATCAGGAAATCAAACTTTTTCTAGTGAAAATTTTTCTTCTAGCGGAACAACGATCAATACCAATAGTCAAGCTCCAGCAGCAACAAATCAATCAGGCTCTACCTCTCAATCATGA", "aa": "MLILGLALLNTFPALASDQKIVIDPINKKILVQSGNQTFSSENFSSSGTTINTNSQAPAATNQSGSTSQS", "aa_hexdigest": "173abe71d3057085ca3c38b5c82e6c0e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7270.015700000003, "isoelectric_point": 5.87637882232666 }, "id": "NKOCPLAKPK_58", "locus": "GCA003260355_00009" }, { "type": "cds", "sequence": "contig_1", "start": 8425, "stop": 8625, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGACTTTCGACAGACAGCGGAAACTTTCGCTATGACGAGGGAGAACAGTCTGTAAAGTCGTTCACTATCGCTGCGGATCTTCTCAGGCACGGAGCCAAAAAAAAGCTCATCATTGATGAGATATTCCGCAATAAAACCTACCGCTCTGTGCAGTTTATGCAGCATTTACTGCAGAGAATGCAAAAAATCAAAATCTGA", "aa": "MGLSTDSGNFRYDEGEQSVKSFTIAADLLRHGAKKKLIIDEIFRNKTYRSVQFMQHLLQRMQKIKI", "aa_hexdigest": "c6ef3f28bfe5d035180b8812c127cb82", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7731.891199999997, "isoelectric_point": 9.895784187316895 }, "id": "NKOCPLAKPK_59", "locus": "GCA003260355_00010" }, { "type": "cds", "sequence": "contig_1", "start": 8902, "stop": 9093, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAGAAGCCATCAAACTCAAACTAGAAAACGCACAAAAAATCGCCCTTTTTGGACACGAACACATTGATGGAGACGCCCTCGGAGCAATTCTAGGCTTGGGAAAACTCCTTGAAAAGCAAGGGAAAACGGTTTCCTATTTCACGCCACACAAACCAAGTCGCGTCTTCGATTTTCTCGAGCTAAACTGA", "aa": "MQEAIKLKLENAQKIALFGHEHIDGDALGAILGLGKLLEKQGKTVSYFTPHKPSRVFDFLELN", "aa_hexdigest": "9f7863c785ad08883b3fa4f97ba4e572", "start_type": "ATG", "rbs_motif": "TTTA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7020.072899999999, "isoelectric_point": 6.821895408630371 }, "id": "NKOCPLAKPK_60", "locus": "GCA003260355_00011" }, { "type": "cds", "sequence": "contig_1", "start": 9285, "stop": 9599, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTCAGTGGAAATGGAATCTTTGGGAAAGCCGAACTCATGCAAAAAACCCTCTACGATGAAACGATCGCCCGGATTGCGGAAGATCTGGACTTTATTTATAGCATTCGAGAGCAGGGAATCCCGATCCTTGTCTTTAGAGATCTAAAAGTCAGACATCAAGAGAGAGAAAAGAGCATCCTCGAACAAGCCCGAATTGGCACCCCAAAAGCAGCAGAGCAGAAAATAAGAAATCTCTTTCTCCGAGCAAAAAAGCACGGGAATCTTTATCAAAAAATCATCTTCATCATACGGAGTTCTCGAGGAATCGCTTGA", "aa": "MFSGNGIFGKAELMQKTLYDETIARIAEDLDFIYSIREQGIPILVFRDLKVRHQEREKSILEQARIGTPKAAEQKIRNLFLRAKKHGNLYQKIIFIIRSSRGIA", "aa_hexdigest": "dfde2f44670ce5a526babd33b6d662e9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12061.95589999998, "isoelectric_point": 9.986297798156738 }, "id": "NKOCPLAKPK_61", "locus": "GCA003260355_00012" }, { "type": "cds", "sequence": "contig_1", "start": 9609, "stop": 10148, "strand": "-", "frame": 2, "gene": null, "product": "Glycosyltransferase 2-like domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4IJ60", "UniRef:UniRef90_A0A328EF09" ], "nt": "ATGGAGAAAACAAGCAAAAATCGAAATATTTCTGTTGTGATTCCTTCTAGAGCGACCCCTCTCCCTCTAAAAAACCTTCTCACCTGCCTCAAAAATCAAACATTTCAAGACTTCGAAATTCTCATCGTCTGTGATAGGTCTTTTACTACATCAGAACGGGACCACTTTCAGCAAGAAATTTATCAAGATCGAAAGCCTGAAGAAACAAACCAAAAAATCAGAATCTTTTCACATCTCAATAGCGATTTCAATAAAGACCATGAAGGAGGGGCAAGCTACGTCAGAAACTTCGGGATCAAAAACGCAAAAGGCAGCTATATTCAGCTTTTTGATGATGATAATGAAATCGATCCACAATACCTTGAACACACCCTCAAGCTACATCAAACCCACACTCAGCGCTATCAAAAAGAAGTAATCATCACACCAACGCTTATGCGGAGAAATACGGACAAAATCCAGAATCAAGGCTTCTCAGACTATAATTATCGACTAGCTCGTCCTATGATCCACTTTCTAAAGCCAGGACAAGCATACTGA", "aa": "MEKTSKNRNISVVIPSRATPLPLKNLLTCLKNQTFQDFEILIVCDRSFTTSERDHFQQEIYQDRKPEETNQKIRIFSHLNSDFNKDHEGGASYVRNFGIKNAKGSYIQLFDDDNEIDPQYLEHTLKLHQTHTQRYQKEVIITPTLMRRNTDKIQNQGFSDYNYRLARPMIHFLKPGQAY", "aa_hexdigest": "ba71def757c3374d6bfc7ec4432c8238", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 9306, "stop": 10148, "observations": { "mutation": [ 9611 ], "stop": [ 9611 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EF09", "query_cov": 1.0, "subject_cov": 0.5359281437125748, "identity": 0.978, "score": 352.0, "evalue": 2.54e-120, "gene_start": 1, "gene_end": 179, "reference_start": 1, "reference_end": 179, "reference_sequence": "MEKTSKNRNISVVIPSRATPLPLKNLLTCLKNQTFQDFEILIVCDRSFTRSERDHFQQEIYQDRKPEEKDQKIRIFSHLNSDFNKDHEGGASYVRNFGIKNAKGSYIQLFDDDNEIDPQYLEHTLKLHQTYTQRYQKEVIITPTLMRRNTDKIQNQGFSDYNYRLARPMIHFLKPGQAYGEIKMFSGNGIFGKAELMQKTLYDETIARIAEDLDFIYSIREQGIPILVFRDLKVRHQEREKSILEQARIGTPKAAEQKIRNLFLRAKKHGNLYQKTIFIIRSSRGIAGRLSFKALWNARNNIPIVKAIRKGYREGWKSFIKTKKSEKNQHFSCN" }, "paralog": false, "identity": 0.9785714285714285, "score": 550.0, "evalue": 1.01e-200, "description": "Internal stop codon at 9611. Nonsense mutation around 9611.", "psc": { "uniref90_id": "UniRef90_A0A328EF09", "product": "Glycosyltransferase 2-like domain-containing protein", "uniref50_id": "UniRef50_A0A2W4IJ60", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EF09", "UniRef:UniRef50_A0A2W4IJ60" ] }, "pscc": { "uniref50_id": "UniRef50_A0A2W4IJ60", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4IJ60" ], "product": "Glycosyltransferase 2-like domain-containing protein" } }, "id": "NKOCPLAKPK_62", "locus": "GCA003260355_00013" }, { "type": "cds", "sequence": "contig_1", "start": 10199, "stop": 10459, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTTACAAGAAGAACTATTGGCTTGTCTTTGGTGGTATTTCCTTGCTTTTGAGTGGTTTTGTTTTTTCTCGGAATTGGGGGAGAGAAATGATGACTACGATTCAGACTTCTTCTGGAGCTTTTTCTTCAATAGCGGATTCAGGTTTTGTTTCTTCTGGAGAAGTTCAGGAAACGACGGATGAAGTTTTGGAAGTTGCTGTCAGTGGACTTAAGTCTACGCCTTTTTGGACGATGAAGGGGGTGGCTCATGATTTCTGA", "aa": "MFYKKNYWLVFGGISLLLSGFVFSRNWGREMMTTIQTSSGAFSSIADSGFVSSGEVQETTDEVLEVAVSGLKSTPFWTMKGVAHDF", "aa_hexdigest": "c3b096e51c4121c5027fad19479284c1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9501.671299999998, "isoelectric_point": 5.123148536682128 }, "id": "NKOCPLAKPK_63", "locus": "GCA003260355_00014" }, { "type": "cds", "sequence": "contig_1", "start": 10598, "stop": 10873, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCCTGAAAATCTTTCAGAACTTTTGGGACTTTTGGAGCTGAACCTTAGCCCTGAAGAGCTCAAAAATCCGAATATTCTCGTGGTTGCACAAACGCAGGAAGGGGTTGTCTTGGCGTATTATCAAGAGGGAAAGCTTAGACTTCTAACCAGTGCTTCTCCTGGGACTCCAAAAACTAAGACGGTCCAGGGAAAGTTTTTTCTCAAGCATGCAAATGTTGCCTATCGCTCTAGGACTTATGGTTCTCCTATGCCTTATGCAATGCGTATTAAGTGA", "aa": "MPENLSELLGLLELNLSPEELKNPNILVVAQTQEGVVLAYYQEGKLRLLTSASPGTPKTKTVQGKFFLKHANVAYRSRTYGSPMPYAMRIK", "aa_hexdigest": "0a6f35cacc4c47f57f694df20c90a04d", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10150.709299999993, "isoelectric_point": 9.520965003967284 }, "id": "NKOCPLAKPK_64", "locus": "GCA003260355_00015" }, { "type": "cds", "sequence": "contig_1", "start": 11206, "stop": 11469, "strand": "+", "frame": 1, "gene": null, "product": "DUF1206 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EFQ6", "UniRef:UniRef90_A0A328EFQ6" ], "nt": "ATGACCGTGATTATGGGGATTTTTTTGGTAAGAGGTAATATTATCTTTGGGGCTGAGCTCTTTAAAGTGCTAAAGGAGGTTTTGATGCCGGGATATTTGGTGTTTTGCGGGATCATGATTGGGTATTTGATCGCTGTGATTTGGCAGGGAAAATTGCCTACTTCGACTGAGGTAATAAACACGAGAGAAAATATTTTTAAGAAGTCCTTTTTGATAGGGGTTTCTTTGGGGGTAGTTTTAGCTGTTTGTTATGTCTTCTACTAA", "aa": "MTVIMGIFLVRGNIIFGAELFKVLKEVLMPGYLVFCGIMIGYLIAVIWQGKLPTSTEVINTRENIFKKSFLIGVSLGVVLAVCYVFY", "aa_hexdigest": "d557f1a20442e4d9834d89edcdd0840b", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A328EFQ6", "query_cov": 1.0, "subject_cov": 0.8613861386138614, "identity": 0.9890000000000001, "score": 166.0, "evalue": 4.41e-52, "valid": true, "product": "DUF1206 domain-containing protein", "uniref50_id": "UniRef50_A0A328EFQ6" }, "pscc": { "uniref50_id": "UniRef50_A0A328EFQ6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EFQ6" ], "product": "DUF1206 domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_65", "locus": "GCA003260355_00016" }, { "type": "cds", "sequence": "contig_1", "start": 11546, "stop": 11983, "strand": "+", "frame": 2, "gene": null, "product": "DUF1795 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MZE0", "UniRef:UniRef90_A0A660MZE0" ], "nt": "TTGTCTCTTGAGGCTTTTGATCTTACATGGACTCCAAAGTTGAAAGTTAAAATGCAGTTGGCTGATGAGGAGCCTGGAGTGTTCTATGTTTATCAAGAGATTGCAGATTCCTTTGAAAACCTCGATACTTTGATTATCGCTCAGGAAACAGGTGTGCTCCTTTCTCTGCCTGGCTATGCCCAGAATGCGATTAATCAGCTGAAGACTGAAGGCTATCTTTTGTCAGGAGAGGTCGCTAAAAAGCTTACGTTTCGTAGTAATGGTCGCTCTTCTTCAGCTTTGCTTAAGAAATATCGCTTGTGGAATAATGAACGTTCGCTCGAAGTAGCTCAGGTTTTTATTCTTGAGGAAGATAGAATGCGTATTCTTTCCTATATGAGTGATGAACCTTCTCACATTAAAAACTTTATATCACAGCTTTCTTCTTTAACCTTTTAG", "aa": "MSLEAFDLTWTPKLKVKMQLADEEPGVFYVYQEIADSFENLDTLIIAQETGVLLSLPGYAQNAINQLKTEGYLLSGEVAKKLTFRSNGRSSSALLKKYRLWNNERSLEVAQVFILEEDRMRILSYMSDEPSHIKNFISQLSSLTF", "aa_hexdigest": "7486d1cd7f87cc3f3fc4c78f33d4535c", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A660MZE0", "query_cov": 1.0, "subject_cov": 0.8285714285714286, "identity": 0.986, "score": 279.0, "evalue": 1.64e-94, "valid": true, "product": "DUF1795 domain-containing protein", "uniref50_id": "UniRef50_A0A660MZE0" }, "pscc": { "uniref50_id": "UniRef50_A0A660MZE0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MZE0" ], "product": "DUF1795 domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_66", "locus": "GCA003260355_00017" }, { "type": "cds", "sequence": "contig_1", "start": 11999, "stop": 12163, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACGAATCCTACTACACCGACAAATTCTGTTTCCTTTACGATTCCTGGTATTGATGGAGGGATGAATCCTCTGAATCCTGGTGCGTTTGCTCGTTCTGAGAGAGAAAAATTAGATCGTCTTGTTAACGCTCCAAGTGCTATTTCGGGTTGGCAGCTTTTGTGA", "aa": "MTNPTTPTNSVSFTIPGIDGGMNPLNPGAFARSEREKLDRLVNAPSAISGWQLL", "aa_hexdigest": "fa0148d9488bee10f0f26b4b87a5d525", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5741.427299999999, "isoelectric_point": 5.971925163269043 }, "id": "NKOCPLAKPK_67", "locus": "GCA003260355_00018" }, { "type": "cds", "sequence": "contig_1", "start": 12497, "stop": 12895, "strand": "+", "frame": 2, "gene": null, "product": "DUF998 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MYQ4", "UniRef:UniRef50_A0A839JTG3", "UniRef:UniRef90_A0A328EJ03" ], "nt": "ATGGCTGAAGTTGTGCTTTTTGCTTTGTATCTTTGTCTTTCACTTTTTCTCTCTTTTTCACAGATGGAGTTTGTGGTGAATCCGAATTATTCGGCTTCTTCTCTCATAGGGAATGCATTAGGGCTTTGTCTCTGTCTTGTGGTGATTGGAATGCTGTGGTCACAAGTTTATTCTAGTGTGGATAAGATGATGCCTTATAGATTGATTTGGTATTCTACTTTGATTTCTTTTCCGTTGATGATCTTGGGCCAGGGTTTGTGGGAGATTATTTATGCAAAGGTATATGAAAATGGAGCCAATCCTTTTTATCTTCCATCACAGGCAGAACTCGATACACAGACGATTTTAGAGGGACAGGAGGAAGAACAAACTCAGGAGAGCGTGAATGTGGAACTTTAA", "aa": "MAEVVLFALYLCLSLFLSFSQMEFVVNPNYSASSLIGNALGLCLCLVVIGMLWSQVYSSVDKMMPYRLIWYSTLISFPLMILGQGLWEIIYAKVYENGANPFYLPSQAELDTQTILEGQEEEQTQESVNVEL", "aa_hexdigest": "7690c3ab0885069cb417cc756732a884", "start_type": "ATG", "rbs_motif": "TTCTT", "psc": { "uniref90_id": "UniRef90_A0A839JTG3", "query_cov": 0.9924242424242424, "subject_cov": 0.8238993710691824, "identity": 0.618, "score": 161.0, "evalue": 2.26e-48, "valid": false, "uniref50_id": "UniRef50_A0A839JTG3" }, "pscc": { "uniref50_id": "UniRef50_A0A839JTG3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A839JTG3" ] }, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 12197, "stop": 12895, "observations": { "mutation": [ 12233, 12410 ], "stop": [ 12233, 12410 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EJ03", "query_cov": 1.0, "subject_cov": 0.4429530201342282, "identity": 0.992, "score": 253.0, "evalue": 1.28e-82, "gene_start": 1, "gene_end": 132, "reference_start": 167, "reference_end": 298, "reference_sequence": "MTNPTTPTNSVSFTIPGIDGGMNPLNPGAFARSEREKLDRLVNAPSAISGWQLLGKIGLGVVIGALMAFVLYIIMGAFGLALGGNASQSTVGAGTAQLTHPLAWLILLFLGFFITFLGNLILVVSYGVFFSQKYTKIGKTIGLLLLTNSILFAGMIALFLFLKEVPMAEVVLFALYLCLSLFLSFSQMEFVVNPNYSASSLIGNALGLCLCLVVIGMLWSQVYSSVDKMMPYRLIWYSTFISFPLMILGQGLWEIIYAKVYENGANPFYLPSQAELDTQTILEGQEEEQTQESVNVEL" }, "paralog": false, "identity": 0.9698275862068966, "score": 441.0, "evalue": 3.65e-159, "description": "Internal stop codon at 12233, 12410. Nonsense mutation around 12233, 12410.", "psc": { "uniref90_id": "UniRef90_A0A328EJ03", "uniref50_id": "UniRef50_A0A660MYQ4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EJ03", "UniRef:UniRef50_A0A660MYQ4" ] }, "pscc": { "uniref50_id": "UniRef50_A0A660MYQ4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MYQ4" ], "product": "DUF998 domain-containing protein" } }, "id": "NKOCPLAKPK_68", "locus": "GCA003260355_00019" }, { "type": "cds", "sequence": "contig_1", "start": 12995, "stop": 13333, "strand": "+", "frame": 2, "gene": null, "product": "ATP synthase subunit I", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EFL5", "UniRef:UniRef90_A0A328EFL5" ], "nt": "TTGGCGATTTTGATTATTGATGCGAATCTTATTTCGGTAAAGGAAGATCCTGCGATTGCTTTGACGCTTGGTCTGATCGGAGTATTCCTTTTTTTTTGGGGGGCGAGCTATTTTTTGTTTTATGGAGTACAGTGGCTTGTTTCTACGAAAATTACTAAGGAAAAAATGCAGAGCGATGCCTATAAATCAGCATTTCTATTTGGGTTATTTGCTCTGATTAATGTATTGCTTTTGCTTGGGAAGTTTTGGACGAAGTGGATAAGTTTGGTTTTGTTAGGGGTGTTTTTTGCAATGCAGTATGTTATTTTTATGGATCCAAAGAGAAAAACTGATGAATGA", "aa": "MAILIIDANLISVKEDPAIALTLGLIGVFLFFWGASYFLFYGVQWLVSTKITKEKMQSDAYKSAFLFGLFALINVLLLLGKFWTKWISLVLLGVFFAMQYVIFMDPKRKTDE", "aa_hexdigest": "bdda7fba668700cdeda538ac3dddb9ad", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A328EFL5", "query_cov": 1.0, "subject_cov": 0.835820895522388, "identity": 0.991, "score": 214.0, "evalue": 4.38e-70, "valid": true, "product": "ATP synthase subunit I", "uniref50_id": "UniRef50_A0A328EFL5" }, "pscc": { "uniref50_id": "UniRef50_A0A328EFL5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EFL5" ], "product": "ATP synthase subunit I" }, "genes": [], "id": "NKOCPLAKPK_69", "locus": "GCA003260355_00020" }, { "type": "cds", "sequence": "contig_1", "start": 13293, "stop": 13628, "strand": "+", "frame": 3, "gene": null, "product": "MIP18 family-like domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EJI3", "UniRef:UniRef90_A0A328EJI3" ], "nt": "ATGTTATTTTTATGGATCCAAAGAGAAAAACTGATGAATGAACTTCAGCATAAGATAGAACAAGCACTTCAGACTGTATATGATCCGGATTTTCCAGTTGTGGATATTTATACACTAGGTCTGATCTATGATATTACTATTGAAGAGCAGAAAATTAAGGTTACTATGTCGTTTACCACGCCGGCCTGTCCTATGGCGGATTATTTGATAGCGAGTGTTGAAGGTGCTGTTCGTTTGGTGGCTCCGGAGTATGAAATTGCTGTAGAGGTTACTTTTGATCCTATGTGGAATCCTCGTATGATTAGGGATCAGGATCTTCAGAGGATGTTTGAGTAG", "aa": "MLFLWIQREKLMNELQHKIEQALQTVYDPDFPVVDIYTLGLIYDITIEEQKIKVTMSFTTPACPMADYLIASVEGAVRLVAPEYEIAVEVTFDPMWNPRMIRDQDLQRMFE", "aa_hexdigest": "9ea7e68fb3d01362e1f7548f646125c2", "start_type": "ATG", "rbs_motif": "TTTTT", "psc": { "uniref90_id": "UniRef90_A0A328EJI3", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.973, "score": 223.0, "evalue": 8.73e-74, "valid": true, "product": "MIP18 family-like domain-containing protein", "uniref50_id": "UniRef50_A0A328EJI3" }, "pscc": { "uniref50_id": "UniRef50_A0A328EJI3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EJI3" ], "product": "MIP18 family-like domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_70", "locus": "GCA003260355_00021" }, { "type": "cds", "sequence": "contig_1", "start": 14733, "stop": 14888, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCGAACCGGTACAGAAATTGCCTCAGTCGGTGACTGCGAAAAAAGTAGTAATCTTTATACTTCCTCATACCAGACAGAAAAATCAGATTTTTCTGGCTGTAAATTACTATTGACAAAAGAATATGCTCCAGATACCGCAATCACACTGCTCTGA", "aa": "MRTGTEIASVGDCEKSSNLYTSSYQTEKSDFSGCKLLLTKEYAPDTAITLL", "aa_hexdigest": "568834bc2f0f9db921c81ba886bb16ef", "start_type": "ATG", "rbs_motif": "TTTA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5568.178399999999, "isoelectric_point": 4.715044593811036 }, "id": "NKOCPLAKPK_71", "locus": "GCA003260355_00022" }, { "type": "cds", "sequence": "contig_1", "start": 14892, "stop": 15161, "strand": "-", "frame": 2, "gene": null, "product": "Type II secretion system protein J", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EJ12", "UniRef:UniRef90_A0A328EJ12" ], "nt": "ATGAAAAAAGCTTTTACTCTAGTGGAAATCATTATCGTAATTGTAATCTTTGCACTACTTTCAGGAGCGATCCTCCGCACCTATACCACCATCACCAAAATTGCCTTTAGAATAGAGCAGGATAAAGAGCTCGCAAAAGAAGTCCTCATTCTTTCACAAGTTCTTCACAATATCGCAGAAGAAGCTACTATTGACTACGAAAAATACCAAGCAAAGGGGATCGACCTCAAACAAGGAAAGGGGATTACTGATACGCTTTATCTCACCTGA", "aa": "MKKAFTLVEIIIVIVIFALLSGAILRTYTTITKIAFRIEQDKELAKEVLILSQVLHNIAEEATIDYEKYQAKGIDLKQGKGITDTLYLT", "aa_hexdigest": "cbf5e6a18485bff404ac93e9f3210f78", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 14589, "stop": 15161, "observations": { "mutation": [ 14735, 14894 ], "stop": [ 14735, 14894 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EJ12", "query_cov": 1.0, "subject_cov": 0.41013824884792627, "identity": 0.9890000000000001, "score": 159.0, "evalue": 1.33e-47, "gene_start": 1, "gene_end": 89, "reference_start": 1, "reference_end": 89, "reference_sequence": "MKKAFTLVEIIIVIVIFALLSGAILRTYTTITKIAFRIEQDKELAKEVLILSQVLHNIAEEATIDYEKYQAKGIDLKQEKGITDTLYLTGGMRTGTEIASVGDCEKSSNLYTSSYQTEKSEFSGCKLLLTKEYAPDTAIILLGNGNINQSKMKFKVIPYLPNNQQDSEIDTGKPGFRILGSIYSAFYNPQRRENKSVFPIQLFLGLQGKTPNIYDIN" }, "paralog": false, "identity": 0.968421052631579, "score": 356.0, "evalue": 1.66e-127, "description": "Internal stop codon at 14735, 14894. Nonsense mutation around 14735, 14894.", "psc": { "uniref90_id": "UniRef90_A0A328EJ12", "product": "Type II secretion system protein J", "uniref50_id": "UniRef50_A0A328EJ12", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EJ12", "UniRef:UniRef50_A0A328EJ12" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EJ12", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EJ12" ], "product": "Type II secretion system protein J" } }, "id": "NKOCPLAKPK_72", "locus": "GCA003260355_00023" }, { "type": "cds", "sequence": "contig_1", "start": 15194, "stop": 15475, "strand": "-", "frame": 3, "gene": null, "product": "Type II secretion system protein J", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EFE9", "UniRef:UniRef90_A0A328EFE9" ], "nt": "ATGGAGAATTTCAGGACCTTTTACCTCAACTTTTTCACTGGAGAAGACAATCAAGAACTAACCTATTATAGCCCACTATCGCTCTCTTCAAGCGAAGAACCCTATAAAGGATTCGCAAGAATCATCGAATATAGCCCAATTTACGTAGGAGATCAGCCTCTTGACAAAAATAAAATACTAAAAATCAGCTCGCGTGTCCTCTACAAAAGAGGCGGGAATACTGGAGAAGTCCTTCTTGAAAGTTTCATTGGAATGAAAGACTCACTCCCAGCCGAACTGTAA", "aa": "MENFRTFYLNFFTGEDNQELTYYSPLSLSSSEEPYKGFARIIEYSPIYVGDQPLDKNKILKISSRVLYKRGGNTGEVLLESFIGMKDSLPAEL", "aa_hexdigest": "18d3e919ab2159dfcff27744b416c8cf", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 15194, "stop": 15775, "observations": { "mutation": [ 15628 ], "stop": [ 15628 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EFE9", "query_cov": 1.0, "subject_cov": 0.44075829383886256, "identity": 0.9890000000000001, "score": 185.0, "evalue": 8.89e-58, "gene_start": 1, "gene_end": 93, "reference_start": 119, "reference_end": 211, "reference_sequence": "MKNLKAFTLIETLIVVFIFGVGILAVLRLLTYSLGYFDSIDMRVKANFLAKEGLEIAYNIRDSKIEQGYTRNYIQEKFEDDTLKQISLGDEGFTNFKVGFSGSGNYYIFEPAEKSENFMENFRTFYLNFFTGEDNQELTYYSPLSLSSSEEPYKGFARIIEYSPIYVGDQPLDKNKILKISSRVLYKRGGNTGEVLLESFIGMKDTLPAEL" }, "paralog": false, "identity": 0.9844559585492227, "score": 379.0, "evalue": 8.12e-137, "description": "Internal stop codon at 15628. Nonsense mutation around 15628.", "psc": { "uniref90_id": "UniRef90_A0A328EFE9", "product": "Type II secretion system protein J", "uniref50_id": "UniRef50_A0A328EFE9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EFE9", "UniRef:UniRef50_A0A328EFE9" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EFE9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EFE9" ], "product": "Type II secretion system protein J" } }, "id": "NKOCPLAKPK_73", "locus": "GCA003260355_00024" }, { "type": "cds", "sequence": "contig_1", "start": 15626, "stop": 15829, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF07963.17" ], "nt": "ATGAAAAATCTGAAAGCCTTCACACTAATTGAAACTCTTATTGTAGTCTTTATTTTTGGAGTAGGTATTTTAGCCGTGCTCCGCTTATTAACCTATAGCCTAGGCTACTTCGATAGCATCGATATGAGAGTAAAGGCAAATTTTCTCGCCAAAGAAGGCTTAGAAATCGCTTACAATATCAGAGACAGCAAAATAGAACAATGA", "aa": "MKNLKAFTLIETLIVVFIFGVGILAVLRLLTYSLGYFDSIDMRVKANFLAKEGLEIAYNIRDSKIEQ", "aa_hexdigest": "22b0fdb6e4c6813f4306084b6a11f3d7", "start_type": "ATG", "rbs_motif": "TTATT", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07963.17", "name": "N_methyl", "length": 27, "aa_cov": 0.40298507462686567, "hmm_cov": 1.0, "evalue": 5.85387973884246e-08, "score": 28.537485122680664, "start": 1, "stop": 27 } ], "seq_stats": { "molecular_weight": 7627.9998, "isoelectric_point": 8.034324073791506 }, "id": "NKOCPLAKPK_74", "locus": "GCA003260355_00025" }, { "type": "cds", "sequence": "contig_1", "start": 15953, "stop": 16405, "strand": "+", "frame": 2, "gene": null, "product": "DUF177 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBFC498", "UniRef:UniRef100_A0A328EF19", "UniRef:UniRef50_A0A660MZW9", "UniRef:UniRef90_A0A660MZW9" ], "nt": "ATGAAAAATCCTGAATTTAAAGTTAAGATCTCGGATCTTTTGATGAAGCAGGCTACGGATACGTTCCGTTTTGAGGGGAAATTTTTGGAAGAAATTCCTCAGCTGACTTCTGATGGAATCTCTGGAGAGATAACGTTGCAATCACTCAATCATAATGCTCTCTTGGTTACTATTGATGCCTTACAATGTACTTTGTGTGAGAGGTGTGATCGCTGTACAAAGGCGTATCTTCGCCCTGTTTCACTATCTGGATATACTGCAAAGTATGTGGTCGATCCGTGCGTTGATGGAGAGGATCCAGAGGAAGATGTCTTGCCTGTTGATGTGAAAAATGGAGTTATTGATTTGCAGGAGCTTATTTATCATGCAATTCAGCTTCAGGAGCCTTTTGTGAAGTATTGTCCTGATTGTGAAAATGCTAGTCTGGAAGAAGAGGATTTTTCAGATTTATAG", "aa": "MKNPEFKVKISDLLMKQATDTFRFEGKFLEEIPQLTSDGISGEITLQSLNHNALLVTIDALQCTLCERCDRCTKAYLRPVSLSGYTAKYVVDPCVDGEDPEEDVLPVDVKNGVIDLQELIYHAIQLQEPFVKYCPDCENASLEEEDFSDL", "aa_hexdigest": "8e559bfa564649291ef2fa515b93cc2d", "start_type": "ATG", "rbs_motif": "TTATT", "ups": { "uniparc_id": "UPI000DBFC498", "uniref100_id": "UniRef100_A0A328EF19", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBFC498", "UniRef:UniRef100_A0A328EF19" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EF19", "uniref90_id": "UniRef90_A0A660MZW9" }, "psc": { "uniref90_id": "UniRef90_A0A660MZW9", "product": "DUF177 domain-containing protein", "uniref50_id": "UniRef50_A0A660MZW9" }, "pscc": { "uniref50_id": "UniRef50_A0A660MZW9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MZW9" ], "product": "DUF177 domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_75", "locus": "GCA003260355_00026" }, { "type": "cds", "sequence": "contig_1", "start": 16530, "stop": 17564, "strand": "+", "frame": 3, "gene": "leuS", "product": "leucine--tRNA ligase", "db_xrefs": [ "EC:6.1.1.4", "GO:0000166", "GO:0004823", "GO:0005524", "GO:0006429", "KEGG:K01869", "SO:0001217", "UniRef:UniRef50_A0A288DNK3", "UniRef:UniRef90_A0A7W4E4B4" ], "nt": "ATGACGATTGATATCAAAACTCTTGCTGGGAAGTGGCAGCGCCAACGAAAAGATCAGAAAATTTATCATACCAAAGAGGATGGAGGGAAACCAAAGTTCTATATTTTGGATATGTTCCCGTATCCGAGTGGGGCTGGGCTTCATGTTGGGCATCCAAAAGGCTATGTCGCATCTGACACGATTGCGAGAAAAAAGATGCTTGAAGGCTATAATGTTCTTCATCCTATGGGATTTGATACTTTCGGACTAGGAACTGAGCAATATGCGATGGATCACAAGATGAAACCTCAGGTGGTCGCTAAACAAAATATTGAAACCTATATCAAACAGCTTGAAAGTATTGGCTTCTCTTATGATCGAGAAAGGAGTTTTAGTACTGCGGATCCCAAGTATTTTAAGTGGACGCAGGCGATTTTTCTCAAGTTTTACAATCATTATTATGATGAAGCTGAGGGGAAGGCAAAACTAATCTCTGAACTTGAAGAAAAACTAAAAAAAGCAGGAAAATCTGAATCAGAAATCAGGTTTATTTTGAATAATGAAAGGTTAGCATATCTTGACTATAAACCTATTAATCGATGTCCTCACTGTAAGACTGGACTTGCAAATGAGGATCTTGATGATGGAAAGTGTGAAAGATGCGGAAGCGAAGTCGAACAAAAACCTATGAGGCAGCGAGTTTTGAGGATTACGAAATATGCTCAGAGACTGCTTGATGGATTGGATGCACTTGACTGGGATGAATCAATGAAAGAGCTTGAGAGAAACCGAATCGGAAAATCCGAAGGTTCACAGTTCAAAATGCAGGTCGTTGCGAGCGAAGCGAAGCAATCCAGTGAAGAACTTGCTTTTGAAGTTTATACTACAAGGCTTGATACTGTGTTTGGGATGAGCTTTGTCGTGATGGCACCAGAGCATCCTTTGGTTGCACAAATCACGACTCCAGAACAGCTAGAGACTGTAAAATCCTATCAAGAACAGGCAAAACATAAAACCCAACTTGAAAGAACTGAACTTCAAAAAGAAAAAACCTGA", "aa": "MTIDIKTLAGKWQRQRKDQKIYHTKEDGGKPKFYILDMFPYPSGAGLHVGHPKGYVASDTIARKKMLEGYNVLHPMGFDTFGLGTEQYAMDHKMKPQVVAKQNIETYIKQLESIGFSYDRERSFSTADPKYFKWTQAIFLKFYNHYYDEAEGKAKLISELEEKLKKAGKSESEIRFILNNERLAYLDYKPINRCPHCKTGLANEDLDDGKCERCGSEVEQKPMRQRVLRITKYAQRLLDGLDALDWDESMKELERNRIGKSEGSQFKMQVVASEAKQSSEELAFEVYTTRLDTVFGMSFVVMAPEHPLVAQITTPEQLETVKSYQEQAKHKTQLERTELQKEKT", "aa_hexdigest": "a9fec0e7cbeab5483bd1a1825a8ef5a5", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00056DAB75", "query_cov": 0.8372093023255814, "subject_cov": 0.8944099378881988, "identity": 0.507, "score": 288.0, "evalue": 1.35e-92, "valid": false, "product": "class I tRNA ligase family protein", "ec_ids": [ "6.1.1.4" ], "uniref50_id": "UniRef50_A0A7Z9PZN2", "kegg_orthology_id": "K01869" }, "genes": [ "leuS" ], "truncated": "3-prime", "pseudogene": { "start": 16530, "stop": 17750, "observations": { "mutation": [ 17562 ], "stop": [ 17562 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A7W4E4B4", "query_cov": 1.0, "subject_cov": 0.4085510688836104, "identity": 0.805, "score": 542.0, "evalue": 2.08e-185, "gene_start": 1, "gene_end": 344, "reference_start": 1, "reference_end": 337, "reference_sequence": "MTIDIKSLAPKWQKKRKEAGVYKTQEGGGKPKYYVLDMFPYPSGAGLHVGHPKGYVASDVIARKKMLEGYNVLHPMGFDTFGLGTEQYAIDHKMKPQVVAEQNIKTYISQLEKIGFSYDWERSFSTADPEFYKWTQMIFLKFYNAYFDEKDQKAKPISELEEKLKKEQKSESEISQILDSERLAYLDYRPINRCPHCKTGLANEDLDDGKCERCGSLVEQRPMRQRVLRITKYAQRLLDGLDNLDRDESMKELERNWIGRSEGSQFKMKIQDSEDYFEVFTTRLDTVFGMSFVALAPEHPLVDQITTTEQKTKVQEYKEQTKHKTQLERTELQKEKTGGFTGAYAINPFNNKQIPVYIGDYVLAGYGTGAVMGVPAHDERDFDFAKKHKLEISEVVIPNSDFSFDFSTMDTLDPQATQKGVLVNSGEFSGLTSDEAIAKMQNWLAERNFGGKKVNYKIQDWVFSRQRYWGEPFPIIFSEGKTIALEESDLPLTLPDIEHYEPTGTEEGPLAEVTEWVNTTTTDGKPARRETNTMPGWAGSSRYWLRYMDPRNNDAFVGKDAEAYWQNVDTYIGGAEHVTRHIIYARFWQKFLYDLGMVSKDEPFQRYQKVGLIMAEDGRKMSKRRGNVINPDDVVAEYGADVLRTYEMFMGPFDQAIAWNTQGMKGVKKFLEKIIALWDKVDLEKKEEKPEIETLLHQTIKKLTIEIDEFKFNTSISQLMILVNKLSEIDQISKQTFEALIIMLAPFAPHLAEEFWEKLGNEFSLFTKGSWPKFDEKKLVSSIITLPIQINGKMRGTLQVSPSLSQDEILNLIKQDEKLANYLTGEIKKCILVPGKIINIIL" }, "paralog": false, "identity": 0.7955665024630542, "score": 654.0, "evalue": 2.42e-231, "description": "Internal stop codon at 17562. Nonsense mutation around 17562.", "psc": { "uniref90_id": "UniRef90_A0A7W4E4B4", "gene": "leuS", "product": "leucine--tRNA ligase", "ec_ids": [ "6.1.1.4" ], "uniref50_id": "UniRef50_A0A288DNK3", "kegg_orthology_id": "K01869", "go_ids": [ "GO:0000166", "GO:0004823", "GO:0005524", "GO:0006429" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A7W4E4B4", "EC:6.1.1.4", "UniRef:UniRef50_A0A288DNK3", "KEGG:K01869", "GO:0000166", "GO:0004823", "GO:0005524", "GO:0006429" ] }, "pscc": { "uniref50_id": "UniRef50_A0A288DNK3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A288DNK3" ], "product": "Leucine--tRNA ligase" } }, "id": "NKOCPLAKPK_76", "locus": "GCA003260355_00027" }, { "type": "cds", "sequence": "contig_1", "start": 17610, "stop": 17798, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13603.12" ], "nt": "GTGCCGATTTATATCGGAGATTATGTGCTTGCCAACTATGGAACAGGAGTAGTGATGGCGGTGCCTGCACATGATGAGAGAGATTTTGACTTTGCGAAGAAGTATGGATTAGAGATAAAAACTTCAGTTTTATATAATGATAAGGACTCTTTAAAGAATAAATTTTTTACATATCCTCGTGCTATATGA", "aa": "MPIYIGDYVLANYGTGVVMAVPAHDERDFDFAKKYGLEIKTSVLYNDKDSLKNKFFTYPRAI", "aa_hexdigest": "57150f49a373193d023ddb7acf3cd72c", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13603.12", "name": "tRNA-synt_1_2", "length": 49, "aa_cov": 0.7903225806451613, "hmm_cov": 1.0, "evalue": 4.386818180393634e-22, "score": 74.9124526977539, "start": 1, "stop": 49 } ], "seq_stats": { "molecular_weight": 7111.0688, "isoelectric_point": 6.530084037780762 }, "id": "NKOCPLAKPK_77", "locus": "GCA003260355_00028" }, { "type": "cds", "sequence": "contig_1", "start": 18021, "stop": 18542, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAGCTTGGCTCGCAGAAAGAGGAATTGGAGGAAAGAAAGTGAACTATAAAATTCAGGATCGGGTATTTTCTAGGCAGAGATATTGGGGAGAGCCGTTTCCTGTAGTGTTCTGTGAGGAGCATGGAGTCGTTCCTTTGCAAGAGTCAGATTTGCCTTTATGTTTGCCTGATGTAGAGCACTATGAACCGACAGGAACTGAAGAAGGACCGCTTGCGGAGGTGAAAGACTGGATTGAAACTACTTGTCCGATCTGCGGAAAACCAGCGAAGAGGGAATCTAATACGATGCCAGGATGGGCGGGCTCGAGCTGGTATTGGATTAGGTATATGGATCCGAATAATGATAATGAGCTTGTTTCATCAGAGAGGGAGCAGTATTGGCAGAACGTAGATACCTATGTTGGTGGAGCGGAGCATGTGACGAGGCATATCATTTATGCGAGATTTTGGCAGAAGTTTTTGTATGATCTCGGACTTGTGAGTAAAGATGAGCCTTTTCAAAAGTATCAAAAAGTCTGA", "aa": "MQAWLAERGIGGKKVNYKIQDRVFSRQRYWGEPFPVVFCEEHGVVPLQESDLPLCLPDVEHYEPTGTEEGPLAEVKDWIETTCPICGKPAKRESNTMPGWAGSSWYWIRYMDPNNDNELVSSEREQYWQNVDTYVGGAEHVTRHIIYARFWQKFLYDLGLVSKDEPFQKYQKV", "aa_hexdigest": "abc67d0df0048cb2e4701f1343b86c83", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI003461EC74", "query_cov": 0.976878612716763, "subject_cov": 0.8622448979591837, "identity": 0.5920000000000001, "score": 228.0, "evalue": 1.29e-73, "valid": false, "product": "class I tRNA ligase family protein", "uniref50_id": "UniRef50_A0A0G1UHJ1" }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 20253.536899999996, "isoelectric_point": 5.148044013977049 }, "id": "NKOCPLAKPK_78", "locus": "GCA003260355_00029" }, { "type": "cds", "sequence": "contig_1", "start": 18549, "stop": 19181, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08264.19", "PFAM:PF00133.28" ], "nt": "ATGGCGGAAGATGGTCGTAAAATGTCTAAGAGACGAGGAAATGTGGTAAACCCTGATGATGTGATTGCTGAATATGGAGCTGATGTCTTCAGGACTTATGAGATGTTTATGGGACCTTTTGATCAGTATATTTCCCGAAATACCAATGGAATAAAAGGAGTGAAGAAGTTCTTTGATAAGCTTATTGCTATTTCAGCTAAAGTCAGTGAGACCTATCAAGATGAGGCAAAAATTCTGACGCTTCTTCATCAGACGATCAAAAAGTTGACGGAAGAGATTGATGACTTTAAGTTCAATACGTCTATTGCTCAGCTGATGATCTTGGTGAATGCTTTATCTGAAGTGGAGCAGGTTTCTAAAGCTACCTTTGAGGCGATTGTGCTGTTGGTTGCGCCGTTTGCACCGCATCTTGCTGAGGAGTTTTGGCAGTTGCTTGGTAATCAGTTTTCAATCTTTACGACGGGAAAATGGCCAAAATATCAAGAGCAATATCTTGTAAATACCGAGATTACGCTTGCAGTGCAGTTTAATGGGAAGATGAGGGGAACTTTGCAAGTGGCGGCTGACTGTAGTCAGGATCAGGTTTTGGATCTTATTAAAAAAGATGAAAAGCTGGCAAGCTATCTCGTCTGA", "aa": "MAEDGRKMSKRRGNVVNPDDVIAEYGADVFRTYEMFMGPFDQYISRNTNGIKGVKKFFDKLIAISAKVSETYQDEAKILTLLHQTIKKLTEEIDDFKFNTSIAQLMILVNALSEVEQVSKATFEAIVLLVAPFAPHLAEEFWQLLGNQFSIFTTGKWPKYQEQYLVNTEITLAVQFNGKMRGTLQVAADCSQDQVLDLIKKDEKLASYLV", "aa_hexdigest": "958a75b105f3d56e32c3eae1ecf4281a", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A7W4EBJ0", "query_cov": 0.8380952380952381, "subject_cov": 0.9025641025641026, "identity": 0.648, "score": 238.0, "evalue": 8.18e-77, "valid": false, "product": "leucine--tRNA ligase", "uniref50_id": "UniRef50_A0A2M7XL11" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08264.19", "name": "Anticodon_1", "length": 142, "aa_cov": 0.5380952380952381, "hmm_cov": 0.9788732394366197, "evalue": 2.684320803940529e-21, "score": 72.46249389648438, "start": 77, "stop": 189 }, { "id": "PF00133.28", "name": "tRNA-synt_1", "length": 33, "aa_cov": 0.15714285714285714, "hmm_cov": 1.0, "evalue": 1.2304205555142942e-09, "score": 33.8151969909668, "start": 1, "stop": 33 } ], "seq_stats": { "molecular_weight": 23863.160600000003, "isoelectric_point": 5.334191703796387 }, "id": "NKOCPLAKPK_79", "locus": "GCA003260355_00030" }, { "type": "cds", "sequence": "contig_1", "start": 19643, "stop": 19861, "strand": "+", "frame": 2, "gene": null, "product": "DNA-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_J9R1I8", "UniRef:UniRef90_A0A328EIT6" ], "nt": "ATGAAATTCAGACTTGTAGAAAAGGCAAATCCGCTTGATAGAAGCAAGAAAAAGTGGTATGCTAATCCCGCGAATACGGGCAAGATGGGGCAAAGGGAAATGTCGAAGCTGCTGGAAGAAAAATCCTCCCTTACGATTGGTGATATTTCTAATGTGATAGAGAATTTGCTTGCTGAACTTCCAAGACAACTGATTGATGGGAAGAGTGTCAGCCTTTGA", "aa": "MKFRLVEKANPLDRSKKKWYANPANTGKMGQREMSKLLEEKSSLTIGDISNVIENLLAELPRQLIDGKSVSL", "aa_hexdigest": "be2182087e2f2f077005bcfda5e9b664", "start_type": "ATG", "rbs_motif": "TTGTA", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 19643, "stop": 20017, "observations": { "mutation": [ 19859 ], "stop": [ 19859 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EIT6", "query_cov": 1.0, "subject_cov": 0.5806451612903226, "identity": 1.0, "score": 140.0, "evalue": 1.12e-41, "gene_start": 1, "gene_end": 72, "reference_start": 1, "reference_end": 72, "reference_sequence": "MKFRLVEKANPLDRSKKKWYANPANTGKMGQREMSKLLEEKSSLTIGDISNVIENLLAELPRQLIDGKSVSLGAFGTFRLTFSSEGTEEKKAFNAASIEPRVVFTPSVEFKERLKHIKYTQETE" }, "paralog": false, "identity": 0.9919354838709677, "score": 240.0, "evalue": 1.47e-83, "description": "Internal stop codon at 19859. Nonsense mutation around 19859.", "psc": { "uniref90_id": "UniRef90_A0A328EIT6", "product": "DNA-binding protein", "uniref50_id": "UniRef50_J9R1I8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EIT6", "UniRef:UniRef50_J9R1I8" ] }, "pscc": { "uniref50_id": "UniRef50_J9R1I8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_J9R1I8" ], "product": "Viral histone-like protein" } }, "id": "NKOCPLAKPK_80", "locus": "GCA003260355_00031" }, { "type": "cds", "sequence": "contig_1", "start": 20176, "stop": 20574, "strand": "+", "frame": 1, "gene": null, "product": "PNPLA domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EJ14", "UniRef:UniRef90_A0A328EJ14" ], "nt": "TTGAAAGCATTGGAGGAACTTGATTTTAAAAAGCAGATAAAGGCGGTGTATGGCGTCAGCGCTGGAGCTCTGACCGGGGCGTATTGGTTGAGTGGATGGCATGCGGAGGCAATTTTTGAAAGGTTTCTGGAGAGTGAGATCCTGGGGCTGAAGAATTTTGCGATTCCTCCCAAGCTTTCTCTTCTCAAAGGGAGTACCGTTGAGGCTTTCTTAAAAAAAGATTTGAAGTCAGATTTTTCTGGCTTGGAAGCTGAGTTTTTTGTCGGAGCTACGGATATTATGACAGCAAAGTTTATTCTTTTTAATGACGGGAAGTTAGTTCCTCCTGTATTGGGGAGTATGGCGGTTCCAGGGCTGTTTCCTCCTGTTGCTTTTGAGCGTCATTTGCTCGTGGATTGA", "aa": "MKALEELDFKKQIKAVYGVSAGALTGAYWLSGWHAEAIFERFLESEILGLKNFAIPPKLSLLKGSTVEAFLKKDLKSDFSGLEAEFFVGATDIMTAKFILFNDGKLVPPVLGSMAVPGLFPPVAFERHLLVD", "aa_hexdigest": "9b70a723b4cda686f02508a2f97b0e3a", "start_type": "TTG", "rbs_motif": "TTTA", "genes": [], "truncated": "both", "pseudogene": { "start": 20095, "stop": 20874, "observations": { "mutation": [ 20140, 20143, 20146, 20572, 20638 ], "stop": [ 20140, 20143, 20146, 20572, 20638 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EJ14", "query_cov": 1.0, "subject_cov": 0.5096525096525096, "identity": 0.992, "score": 264.0, "evalue": 1.42e-87, "gene_start": 1, "gene_end": 132, "reference_start": 28, "reference_end": 159, "reference_sequence": "MMIKKEKSDFILVISGGGAKGIYACGILKALEELDFKKQIKAVYGVSAGALTGAYWLSGWHAEAIFERFLESEILGLKNFAIPPKLSLLKGSTVEAFLKKDLKSDFSGLEAEFFVGATDIMTAKFILFNDGKLVPPVLGSMAVPGLFPPVAFERHLLVDGGVLNNFPVDFARKAYPDAKIIGILLGKFKQHQKVTSLLDVLSLSYSVALQGRLSKNLDKVDYLLYKDLEVGMVESSEKKLRALFKQGYEDGLKAFSSQK" }, "paralog": false, "identity": 0.9806949806949807, "score": 488.0, "evalue": 3.39e-178, "description": "Internal stop codon at 20140, 20143, 20146, 20572, 20638. Nonsense mutation around 20140, 20143, 20146, 20572, 20638.", "psc": { "uniref90_id": "UniRef90_A0A328EJ14", "product": "PNPLA domain-containing protein", "uniref50_id": "UniRef50_A0A328EJ14", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EJ14", "UniRef:UniRef50_A0A328EJ14" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EJ14", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EJ14" ], "product": "PNPLA domain-containing protein" } }, "id": "NKOCPLAKPK_81", "locus": "GCA003260355_00032" }, { "type": "cds", "sequence": "contig_1", "start": 20674, "stop": 20874, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGACTTCTCTTTTAGATGTTTTGTCGCTTTCTTATTCGGTTGCTTTGCAAGGGCGCTTGAGTAAAAATTTGGATAAGGTGGATTATCTTCTTTATAAAGATCTTGAGGTAGGAATGGTTGAGAGTTCTGAAAAGAAACTGAGGGCTCTTTTTAAGCAGGGGTATGAAGATGGTCTGAAAGCTTTTAGTTCTCAAAAGTAA", "aa": "MTSLLDVLSLSYSVALQGRLSKNLDKVDYLLYKDLEVGMVESSEKKLRALFKQGYEDGLKAFSSQK", "aa_hexdigest": "1dd73b2bdf42f058a9ab1eb083f69f9c", "start_type": "GTG", "rbs_motif": "TTTA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7448.523, "isoelectric_point": 7.9425855636596685 }, "id": "NKOCPLAKPK_82", "locus": "GCA003260355_00033" }, { "type": "cds", "sequence": "contig_1", "start": 20886, "stop": 21047, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01734.28" ], "nt": "TTGAAGTTTCCTTTTTTGAGCGCTTTGACCCCAAAAGAGCGTTATGTCCTTGTTATTTCTGGTGGAGGAATGAGGGGTTTTTATGGCTTGGGGATTGTGAGGGCACTAGAGGAATTAGGATTTAAGGATAGAATAGATGCGCTGTATGGTGTGAGTGCCTGA", "aa": "MKFPFLSALTPKERYVLVISGGGMRGFYGLGIVRALEELGFKDRIDALYGVSA", "aa_hexdigest": "f954d4767266bb438a1b407d012c0273", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01734.28", "name": "Patatin", "length": 37, "aa_cov": 0.6981132075471698, "hmm_cov": 0.972972972972973, "evalue": 1.263982168848513e-09, "score": 35.021400451660156, "start": 17, "stop": 53 } ], "seq_stats": { "molecular_weight": 5810.7868, "isoelectric_point": 9.299516105651854 }, "id": "NKOCPLAKPK_83", "locus": "GCA003260355_00034" }, { "type": "cds", "sequence": "contig_1", "start": 21051, "stop": 21338, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTAGTAAGTTATCGAGCGGCGGGGTATTCTGCGGATAAGATTCTTTCACTGTTCCTTAAGTCTGAATTCCTTTCTCTGACGAAAAATCTGAATTTTCTGCCTAAGGTTTCTCTCCTTAAGAATACTTTACTCAAAAAGCAGATGGAAAAGGAGCTCCCTAAGAGCTTTGAAGAGCTTAAAATTCCAACTTTTATTGGTTGTACAGAAATCAAAAAGGGAGAAAGTCTGATCCTTTCAGAAGGAGACCTCGTTTCTGCACTTTTAGGGACGATTGCTTTGCCGTGA", "aa": "MLVSYRAAGYSADKILSLFLKSEFLSLTKNLNFLPKVSLLKNTLLKKQMEKELPKSFEELKIPTFIGCTEIKKGESLILSEGDLVSALLGTIALP", "aa_hexdigest": "aad339afc42701ef3ce2a59217357547", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10518.432599999987, "isoelectric_point": 9.068719291687014 }, "id": "NKOCPLAKPK_84", "locus": "GCA003260355_00035" }, { "type": "cds", "sequence": "contig_1", "start": 21387, "stop": 21683, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGACGAATAATTTTCTCGTAGAGCCTGCAAAGAAAGCCTATCCTCGCCATAAAATTATCGGGATTTCTCTCAACAAATATCGCACTAATCCCAAGGTAAAAAATCTGCTCGATAGCTTGATGGTTTCTTTTGAAATTATGCTGAAAAAGGATATTCCGGAGCAGGGAAGTTTGAGTGATATTTTCTTTTGTAGGGATTTGGATACGCAGGTACTGGAGTTTACTACGACGAAACTTAAAAAGATATTTAAGCTCGGATATGAAGATGGTTTGGAGAAGCTTCGTGCTTTAGGTTAG", "aa": "MTNNFLVEPAKKAYPRHKIIGISLNKYRTNPKVKNLLDSLMVSFEIMLKKDIPEQGSLSDIFFCRDLDTQVLEFTTTKLKKIFKLGYEDGLEKLRALG", "aa_hexdigest": "6fb4a76a6d44439dc412e8b7c1994c61", "start_type": "GTG", "rbs_motif": "TTATT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11309.204899999986, "isoelectric_point": 9.410401725769042 }, "id": "NKOCPLAKPK_85", "locus": "GCA003260355_00036" }, { "type": "cds", "sequence": "contig_1", "start": 21675, "stop": 22247, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGCAATCCCAAAAAATGCTCCAACTGGATTACTTCGCTCCACTCGCAAAGACGCACAGCCAGCTTCTTTCCAAACCTATCTTCTCGGGAAAGCACCTGAGCTTTCAAGTTTTCGTCATTTCGTGAGCCTTGATACCAGAAGCAGAGAGGTAAGGAATAAAAAAGAAAAAACTGAAGTTCTGAATTTGGTTGTGAGGGCTTATGAAAGCTCAGTTGGACAAGAATATTTCATCAGTTATGAAGATGAACTCGGTTATCTCTATGGATTTACGAGGCTTCTTTCACCAAAACTTGAGGAAAGACTGGATATTTCAGGACTAGGTTTGGAAACAGCTTTGATTAGAGAGCTTCATGTTTATGGTTCTTTACAGAGTTTGAAAACACAAGAGGAAAGCGGGCAGAAAGTGCAGCATAGCGGCTTGGGGAAACAGCTCCTTGAAACCGCAGAAAAAATCGCTCAAAAATCGGGCTTTTCAAAGCTGTCTGTGATTTCTGGAGTTGGAGTGAGGGAATACTATCGCAAGCAGGGATATAAACTAGAGGGAACCTATATGGTGAAAACACTAACCTAA", "aa": "MAIPKNAPTGLLRSTRKDAQPASFQTYLLGKAPELSSFRHFVSLDTRSREVRNKKEKTEVLNLVVRAYESSVGQEYFISYEDELGYLYGFTRLLSPKLEERLDISGLGLETALIRELHVYGSLQSLKTQEESGQKVQHSGLGKQLLETAEKIAQKSGFSKLSVISGVGVREYYRKQGYKLEGTYMVKTLT", "aa_hexdigest": "d95a9b1ec21daffb40f95f325227f57d", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 21419.188400000003, "isoelectric_point": 9.354314231872557 }, "id": "NKOCPLAKPK_86", "locus": "GCA003260355_00037" }, { "type": "cds", "sequence": "contig_1", "start": 22251, "stop": 22952, "strand": "-", "frame": 2, "gene": null, "product": "Radical SAM core domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A351Y4G5" ], "nt": "TTGGAAGAGGCAATACAGATCAACGAAACCGCACAGCACAGAATTATCGGATTGACCATAGAAACCAGGCCAGAATTTGTGACTGATCAGAACTGTAGAGATCGGAGAGCGATGGGCGTAACGAGGATTGAAATGGGCGTGCAGTCTACTGATGATGCAGTTTTGGACTTGAATAAAAGAGGACATCATCTCGCAGAGGTAGAAAAGGCACTTCACAAGCTCAGGCAATATGCTTTCAAGTTTTCTATTCACATTATGCCGGGGCTTTATGGTTCTACGATGGAAAAGGATATTCAGACTTTTCGTGATGTGTATACCAATTCCTACCTCAAGCCTGATGAAATTAAGTTGTACCCAACTTCGGTGATCCCGCAAACGGAACTGTATGAGCTCTATCAGCAGGGGAAGTATGAGCCGATCACGACAGAGGAGATTAGTGAGATTATTGAGACGACTTTTCGTGAGATTATTCCGCCCTATACGAGGATCAAGAGGCTGATTCGTGATATTCCTGCTACGGAAATTAGTGCTGGATCTAATGTTACGAATCTTTCTCAGCTTATGCATGAAAAGCTTTTGAAAAAGTATCAAAAAGCTGATCCAGACTTCCGTTCTATTTTTTATCACAGACTCTATGAGAATTTGCAAGTTTTTGGTGATGAAGAGGAGTTTTTTTCAGTCATTACGAACGGAACGAAATGA", "aa": "MEEAIQINETAQHRIIGLTIETRPEFVTDQNCRDRRAMGVTRIEMGVQSTDDAVLDLNKRGHHLAEVEKALHKLRQYAFKFSIHIMPGLYGSTMEKDIQTFRDVYTNSYLKPDEIKLYPTSVIPQTELYELYQQGKYEPITTEEISEIIETTFREIIPPYTRIKRLIRDIPATEISAGSNVTNLSQLMHEKLLKKYQKADPDFRSIFYHRLYENLQVFGDEEEFFSVITNGTK", "aa_hexdigest": "bcefc1a8a26ce8c61f7138bf5dbd15c4", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A351Y4G5", "query_cov": 0.9098712446351931, "subject_cov": 0.848, "identity": 0.637, "score": 287.0, "evalue": 3.09e-95, "valid": false, "product": "Radical SAM core domain-containing protein", "uniref50_id": "UniRef50_A0A351Y4G5" }, "pscc": { "uniref50_id": "UniRef50_A0A351Y4G5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A351Y4G5" ], "product": "Radical SAM core domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_87", "locus": "GCA003260355_00038" }, { "type": "cds", "sequence": "contig_1", "start": 23043, "stop": 23570, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCACTTTCCGCTCTCGTTCTCACTCTCTTACAAGAAAAAAATCTGACTTCCGAATCTTTGAAAGATGCCCAGAGAGCTTTTGCTAAATCTCATAATTTAAGCACCCTTCCGAGTAAATCACAGATTCTCCAAGCATATTTTCAGCTTTTGGAAGAAGGAAAAATAGAAAAGAATGCTGATTTTGAGCTTTTGCTGCGTAAAAGGGCAATTCGTTCGCTTTCAGGGATCGTTCCGATTCAAGTTTTGACCAAGCCTTTTCCTTGTCCTTCAAGATGTATTTTCTGCCCCAATGATCCGGAGATGCCCAAGAGCTATATCAAGTCTGAACCTGGGGCAATGAGAGCTTTTCTCAACCAGTTTGATCCGCTCAAGCAGGTCTACAATAGGCTTTACTCTCTCCAGCAGACGGGGCATAAGACCGATAAGATTGAGATGATTGTGCTGGGGGGAACTTGGGATTTCTATCCGAGAGACTACAAAGTTGACTTTATCAAGCAGCTCTATGATGCCTGCAATACCTTTTGA", "aa": "MPLSALVLTLLQEKNLTSESLKDAQRAFAKSHNLSTLPSKSQILQAYFQLLEEGKIEKNADFELLLRKRAIRSLSGIVPIQVLTKPFPCPSRCIFCPNDPEMPKSYIKSEPGAMRAFLNQFDPLKQVYNRLYSLQQTGHKTDKIEMIVLGGTWDFYPRDYKVDFIKQLYDACNTF", "aa_hexdigest": "ce7fb5195c54f6067bd55fc474bf2c2e", "start_type": "ATG", "rbs_motif": "TTATT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 20096.12399999999, "isoelectric_point": 9.000640678405762 }, "id": "NKOCPLAKPK_88", "locus": "GCA003260355_00039" }, { "type": "cds", "sequence": "contig_1", "start": 23751, "stop": 24071, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTGCGGAGGCACCGTCGATCCCCCCGCTACGACAAGCATCATTAAAGAGGGAATCACCGATCATCTCTTCTATGGCATCTCTGGTAGTGATTTCTACATCCTAAGTATCACCAATACCGCTAATGTAGCACTCACTTTTGAAACTACCGAATACTATAACGAGGGAGGGAAAGGACGCTCTAGGAAAGAAACCATTCCTCCCCACAGAACGATCACCAAACTCGGCAATACAAGAAGGAAAGTTTCACTTCTTAGGGTTAGCATAAAAAACGCCTCACCGAGTGGGACCATCGCTTTAGCGACCGGCATGAGACAGTAA", "aa": "MCGGTVDPPATTSIIKEGITDHLFYGISGSDFYILSITNTANVALTFETTEYYNEGGKGRSRKETIPPHRTITKLGNTRRKVSLLRVSIKNASPSGTIALATGMRQ", "aa_hexdigest": "a5fdb3cc35d213e73dd6ba0211327217", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11463.916199999981, "isoelectric_point": 9.66247310638428 }, "id": "NKOCPLAKPK_89", "locus": "GCA003260355_00040" }, { "type": "cds", "sequence": "contig_1", "start": 24426, "stop": 25010, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DC027F4", "UniRef:UniRef100_A0A328EFF9", "UniRef:UniRef50_A0A328EFF9", "UniRef:UniRef90_A0A328EFF9" ], "nt": "ATGGCTCAAAACACAACATCTTATCAATTTTTCAAAAAAAGGGTTGATTTCACTCCCCCAAATCACTATAGTCTCTCCCGCTGTCCAAAAAAAAAGGCACATTTAACAAATAAGATGAAAAAAATCACAGTATTGGCAATATTGATTGCCCTCTTCGCTAGTGTTAGCGAAATGTATGCGAATGACCCTAAGGTTTCAAACCTTGTCTCGGATGCCCCAACTTCTCCGGTCAAGAAAGAGAGGAAAGTCCTCTGTTTTCTGTCCAAGAGCTGGCAATTCCATGCAAATTCGGAGAGTTCCCACCGATTCACGAAGATCAACGGTGATGATCTTTATGTCCTCAGTGTGGAGAACACTTCTGACGCACCGATTACCATCCAAGTCATTGAATATGACAAAGCTGGTAAGACTCTCGGCTCACGCGAGCTTGCAATCTCAGTGGGAAAACCTTTCACTGAAGTCTATGATACAGAGAATAGGATTTTCTCTGTAGGTGTCAAGATACTTACTCCTTCTGCAAAAGGAAGAGTATCAGTAGCTCACGGTGTAAACCCTGAGAGAACAAATACAAATAAACTTAATTAA", "aa": "MAQNTTSYQFFKKRVDFTPPNHYSLSRCPKKKAHLTNKMKKITVLAILIALFASVSEMYANDPKVSNLVSDAPTSPVKKERKVLCFLSKSWQFHANSESSHRFTKINGDDLYVLSVENTSDAPITIQVIEYDKAGKTLGSRELAISVGKPFTEVYDTENRIFSVGVKILTPSAKGRVSVAHGVNPERTNTNKLN", "aa_hexdigest": "e58572061755dc29beb5807ca5b2cb74", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000DC027F4", "uniref100_id": "UniRef100_A0A328EFF9", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DC027F4", "UniRef:UniRef100_A0A328EFF9" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EFF9", "uniref90_id": "UniRef90_A0A328EFF9" }, "psc": { "uniref90_id": "UniRef90_A0A328EFF9", "uniref50_id": "UniRef50_A0A328EFF9" }, "pscc": { "uniref50_id": "UniRef50_A0A328EFF9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EFF9" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 21638.544, "isoelectric_point": 9.672401237487794 }, "id": "NKOCPLAKPK_90", "locus": "GCA003260355_00041" }, { "type": "cds", "sequence": "contig_1", "start": 25536, "stop": 25715, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATCTCATAAAGCTTGTCATATGTCTGAGTAGTTATTCCCTTTCCCTCAAAGTTTGTATTGTCTGCTCGAAGACGGAACTCAATATCCTTACTTTCTTCTGGCTCTCGGTAGTAGTCCTCAATTCAAAGATAGTCACGGATCTCGTAGCCATAAAATCGGCTACTGTCTTTATCTGA", "aa": "MNLIKLVICLSSYSLSLKVCIVCSKTELNILTFFWLSVVVLNSKIVTDLVAIKSATVFI", "aa_hexdigest": "3df0bf32560e9cfaf1ce02714a541561", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6535.9632, "isoelectric_point": 8.98768253326416 }, "id": "NKOCPLAKPK_91", "locus": "GCA003260355_00042" }, { "type": "cds", "sequence": "contig_1", "start": 25662, "stop": 26564, "strand": "-", "frame": 2, "gene": null, "product": "DUF4153 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EF30", "UniRef:UniRef90_A0A328EF30" ], "nt": "TTGGCGGGACTTATTGTTTGGGGAGGACTCTCTGGAGCTCTTGGCTCAATCAGCGAACTCTTTGACGTAGATATCAACCGAAAATGGTATGAATATTTTGGAACATTCAGTATGTTCCTACTCGCAGGAAGCTTTATGATCAATTATTATAGCTCCGTCACAGAATACCTCCCCAAAGGCAGCCATTCAGAATTTGAACTCCAATCTACCCGCTTGATGAAAATCTTTGGAAGCTATATTTTCCTTCCGCTCGCACTGATCTATCTGACAATATTTACCGCTTATGGAATAAAAATTCTCATCACTGGCGTTCGACCTAAAGGCATCATCGTGTGGTTGGGAACAGGATATTTTGCTCGAGGAATGCTGACGTACTTCCTTACATTCGCAGAAAATTCCAGGTTCTTTGATAAGATCCGCCAAATCCTCTTCATCAGTTTCGTCTTGATTGCATTGATGATGATCAAAGCTCTCCAACTCAGAATCCAAGACTACGGCATTACTATAAATAGATATTTCGTAGGGATGTTTATCCTCTTTATCATAGGATTCTCAGTCTTGGCACTTTGCTTACCAAAGATGAGGCTCAGACTTTTCATCTCGTTGCTCTTCGGACTGAGTCTAATTTCCCTCTACAGCCCACGAAACGCACCAACCATCGCCTTCTCTTCCCAGAAATCCCGTATTGAATCCCTCCTCGCCAAAGCAAACCTCAAGCTTCCTTTGCAAAAAGATAGCTTCAAAGACTTGACTGGAGATGGAGCAGGAGAGTTGACGGAAACCGTTATCGACTTTGTAAATACTCAGCCTTACGAAAAACGAAATAAGGTCCTCTTCAATAACCAATATTCAGATAAAGACAGTAGCCGATTTTATGGCTACGAGATCCGTGACTATCTTTGA", "aa": "MAGLIVWGGLSGALGSISELFDVDINRKWYEYFGTFSMFLLAGSFMINYYSSVTEYLPKGSHSEFELQSTRLMKIFGSYIFLPLALIYLTIFTAYGIKILITGVRPKGIIVWLGTGYFARGMLTYFLTFAENSRFFDKIRQILFISFVLIALMMIKALQLRIQDYGITINRYFVGMFILFIIGFSVLALCLPKMRLRLFISLLFGLSLISLYSPRNAPTIAFSSQKSRIESLLAKANLKLPLQKDSFKDLTGDGAGELTETVIDFVNTQPYEKRNKVLFNNQYSDKDSSRFYGYEIRDYL", "aa_hexdigest": "b39d8ffb530431a9a1fbe8512efcb2ad", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 25359, "stop": 26864, "observations": { "mutation": [ 25487, 25664, 26570, 26684, 26843 ], "stop": [ 25487, 25664, 26570, 26684, 26843 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EF30", "query_cov": 1.0, "subject_cov": 0.5217391304347826, "identity": 0.96, "score": 547.0, "evalue": 1.11e-191, "gene_start": 1, "gene_end": 300, "reference_start": 154, "reference_end": 453, "reference_sequence": "MKIKQFFGNRINGLKTFPVAHLILLFLTGCSTYFIHIDQNETRQIKLIVAGILGFLVSLIGPLLTLHAEEETKTIRIINRITQLFALPTIAGYYRLLQGLSENTHYAENLLYFGIFPLLILGILTLIARIFRQKEHKIWFSRTSLLHSVGFGLLAGLIVWGGLSGALGSISALFDVDINRKWYEYFGTFSMFLLAGSFMINYYSSVTEYLPKGSHSEFELQSTRLMKIFGSYIFLPLALIYLTIFTAYGIKILITGVRPKGIIVWLGTGYFARGMLTYFLTFAENSKFFDKIRQILFISFVLVALMMIKALQLRIQDYGITINRYFVGMFILFIIGFSVLALCLPKMRLRLFISLLFGLSLISLYSPRNAPTIAFSSQKSHIESLLAKANIKLPLQKDSFKDLTGDGAGELTETVIGFVNTQPYEKRNKVLFNNQYSGRDSSRSYDYEIRGYLGIENYHREPEESKDIEFRLRADDTSFEGKGITTQAYDKLYDLHWTSPLIRDNTLMYSISGEIKEINLTPYLEEIFAQSKRSGEPFIIEQENTKFIITRLNGTKFENGKIQLDNEIEGYLLTK" }, "paralog": false, "identity": 0.9481037924151696, "score": 930.0, "evalue": 0.0, "description": "Internal stop codon at 25487, 25664, 26570, 26684, 26843. Nonsense mutation around 25487, 25664, 26570, 26684, 26843.", "psc": { "uniref90_id": "UniRef90_A0A328EF30", "product": "DUF4153 domain-containing protein", "uniref50_id": "UniRef50_A0A328EF30", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EF30", "UniRef:UniRef50_A0A328EF30" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EF30", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EF30" ], "product": "DUF4153 domain-containing protein" } }, "id": "NKOCPLAKPK_92", "locus": "GCA003260355_00043" }, { "type": "cds", "sequence": "contig_1", "start": 26961, "stop": 27107, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGCAACGGGGAAGGTCTTGAGTCCGTTTATTCGGTTTCAGAAGAATTGTTTGATCTTCATCTGGTTTTGAAAAAGGATAAAAAAGTAGTTTATGTTTACGAAAGTTTTTTACTGAGTCAAGGGAAGTTTGCTGGCGGTGGTTGA", "aa": "MSNGEGLESVYSVSEELFDLHLVLKKDKKVVYVYESFLLSQGKFAGGG", "aa_hexdigest": "21ae0991829372f89c69102d182599bf", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5298.9684, "isoelectric_point": 5.090295600891112 }, "id": "NKOCPLAKPK_93", "locus": "GCA003260355_00044" }, { "type": "cds", "sequence": "contig_1", "start": 27128, "stop": 27637, "strand": "-", "frame": 3, "gene": null, "product": "Methylated-DNA--protein-cysteine methyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MTX4", "UniRef:UniRef90_A0A660MTX4" ], "nt": "ATGATAGCAATAAGCCACTACGAATCACCGCTAGGCACAGTCCTGCTTGCCGCCAAAGAAAAGTCCCTCATCGGTCTACGAATCACCCCACAAACCGCAGATCTCAGCAACTATCATGAACCAATCCAAGAGCAACCCCAAAATCCCGTCCTCTTAGCAGCCAAATCTCGACTAGATCGCTACTTTGCAGGAGAAAAACCATTGATAAACGAACTTCCTTTGAATCCGATTGGCAACGAATTCAGCCAAACCGTCCGAAACCTACTCAAAACCATTCCTTACGGCAAAACTACCAGCTACGGAGCCATTGCTCAGCAAGTAGCCTCCATTAGAGGTATCAAAAAGATGTCGGCCCAAGCGATCGGAGGAGCAGTAGGAAGAAATCCTATTGCAATCATCATTCCCTGCCATAGAGTTATAGGAAAATCCGGAGCCCTCGTCGGCTATACTGGAGGTCTTGATCTAAAAATCGCCTTGCTTCAGCATGAAAGTAACAATTCCTTTCCCTGA", "aa": "MIAISHYESPLGTVLLAAKEKSLIGLRITPQTADLSNYHEPIQEQPQNPVLLAAKSRLDRYFAGEKPLINELPLNPIGNEFSQTVRNLLKTIPYGKTTSYGAIAQQVASIRGIKKMSAQAIGGAVGRNPIAIIIPCHRVIGKSGALVGYTGGLDLKIALLQHESNNSFP", "aa_hexdigest": "e26e417a3f48626f2c30028fa09345b3", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A660MTX4", "query_cov": 1.0, "subject_cov": 0.8802083333333334, "identity": 0.9640000000000001, "score": 320.0, "evalue": 2.72e-110, "valid": true, "product": "Methylated-DNA--protein-cysteine methyltransferase", "uniref50_id": "UniRef50_A0A660MTX4" }, "pscc": { "uniref50_id": "UniRef50_A0A660MTX4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MTX4" ], "product": "Methylated-DNA--protein-cysteine methyltransferase" }, "genes": [], "id": "NKOCPLAKPK_94", "locus": "GCA003260355_00045" }, { "type": "cds", "sequence": "contig_1", "start": 28071, "stop": 28208, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGAGAGGATGCTCCTTTGGAACTGCTTGATAATGCGGGTGATTTGGCTGGACTGCAAAGTCAAGATGCTGCTTATGTCGTAAAAAGATATAATACGCAATTTATTCTTGCTGTTTTGGGAATTATCGGGCTTTGA", "aa": "MGEDAPLELLDNAGDLAGLQSQDAAYVVKRYNTQFILAVLGIIGL", "aa_hexdigest": "1bb99f117f9d4823859946bcf9586fd9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4777.406799999998, "isoelectric_point": 4.0500284194946286 }, "id": "NKOCPLAKPK_95", "locus": "GCA003260355_00046" }, { "type": "cds", "sequence": "contig_1", "start": 28245, "stop": 28397, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGATTTGAAGTCTCAGCCTACGGTGCCGGTAGATAAGATCGAACGTGTGCAAAAAGTCTCTGATACCTATGATCAGTATGGAACGATGTTTGGGCTTTTTGATAAAGCTAAGTATGCTACACTTTCTCTTGCAGAGCAGGGAGAGACTTGA", "aa": "MDLKSQPTVPVDKIERVQKVSDTYDQYGTMFGLFDKAKYATLSLAEQGET", "aa_hexdigest": "b609c6e1c52ac1b0f7cfef28622cc0a6", "start_type": "TTG", "rbs_motif": "TTATT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5644.2989, "isoelectric_point": 4.716181373596191 }, "id": "NKOCPLAKPK_96", "locus": "GCA003260355_00047" }, { "type": "cds", "sequence": "contig_1", "start": 28434, "stop": 28700, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCAGAAGAAAGATATTTTGCAAACCAAGCTTTCTGCTTTAGGAGAGCAGATTATTGCTCTAAAAACACAGACAAAAGCTTTCCAAGAAAGCATGAATAAAGATGGATTCTTTCCTCAAGAATTAGTCGATATTTTGAAAGCAGATAATGCTGTGGGATCTATTCAGAGAAGTTTACTTTCTCTAGAAGTAGTGAAATTTTCGAGTGCGATGAAGGTGTTTTCACTTATGGATGTTTTTGTTTCTTCCTTGGCGGAGAATCTTTGA", "aa": "MQKKDILQTKLSALGEQIIALKTQTKAFQESMNKDGFFPQELVDILKADNAVGSIQRSLLSLEVVKFSSAMKVFSLMDVFVSSLAENL", "aa_hexdigest": "08e5074c3caa2058d0fbdf5601763bc1", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9753.292999999998, "isoelectric_point": 6.175920295715332 }, "id": "NKOCPLAKPK_97", "locus": "GCA003260355_00048" }, { "type": "cds", "sequence": "contig_1", "start": 28740, "stop": 29330, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATAGAGGAGAAAAGGATGTAGAATACTATCTTAATGTCTGCTATAATAATCCTTATGAGCCTACAAAATGTGATAAAATTAATGATTTCGATGAGTATTATAAGAAGATTCTTGGTGATAGTTCCTTTGATACCGGATTCTTCAAGATTCTCATGGAGTTTATTAATAATAGGTTAGAATTTAGTAATATGCCGAGCTTTTCTATCACCTTCAACAGCTTTAATGGTAAGAGTAATAGTATTGGCTTTACTATCGAAGTGAATACTCTTAAGGAAGATGAATATGCGCTTATTCAGCAAGGTATTAAGAGTCCTCATATCTTTATTATCTCTGAATTGATTAAGTTGCTCAAGCAAAGTACCTTTATTGTGGGGAAGGCAATTGATGCAAAAGATATTAAGGTTTCTCCAAAGCAGGTTACTATTGGAGGATCGAAATATATCGTGAATAACTCAGTAAAAACCTTTAATCTTCCAATTCAAAAGACGACTGAGAGGGAAATCTTTGACTTTATGGATAGTTATATTTCAAGAATTAAGGATTCTCAGACGGATACTGGAGCTCTTGCTTTTAGTGGTGGGTTTTGA", "aa": "MDRGEKDVEYYLNVCYNNPYEPTKCDKINDFDEYYKKILGDSSFDTGFFKILMEFINNRLEFSNMPSFSITFNSFNGKSNSIGFTIEVNTLKEDEYALIQQGIKSPHIFIISELIKLLKQSTFIVGKAIDAKDIKVSPKQVTIGGSKYIVNNSVKTFNLPIQKTTEREIFDFMDSYISRIKDSQTDTGALAFSGGF", "aa_hexdigest": "446500b12717176c1a6761cdd218c482", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 22374.140799999997, "isoelectric_point": 5.3109445571899405 }, "id": "NKOCPLAKPK_98", "locus": "GCA003260355_00049" }, { "type": "cds", "sequence": "contig_1", "start": 29551, "stop": 30807, "strand": "+", "frame": 1, "gene": "ppiC", "product": "PpiC domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7W4HGX6", "UniRef:UniRef90_A0A660MTQ3" ], "nt": "ATGCAGAAAGGGAAAAATGGCAGTAAGCTAGAAATCTCTTTTGGAATTAATAAACAGACCTATCTCGTTACGCTTGGAATCTTTGGGGTAATGTCTGTGGTCACGGTTATGGCTCTTTTCTTTATCTGGTCTAAAAACTCTGAGCTGACAGCAAGGACTTGGGATCTCAAAGAACTTAATAAATATGATTTGACGAGCTTTCAACAGTCAGAGGTAATGGCTGAAGATAAGGATTCTCTGCCTCAAACTATTGAGGATATTGTTGGTTTGTATTCTTCTTCTAAAGAAGAAAAGGAAAGAATTGAAGGAGAGCTGACTTTTAAAAAGAGTGTTTATACTGAATTTTTGAGAAATCTTCTTTTACCTTCGCTTAATATCTGGAAAGATCCGTATACGAAGGATATCGATATTTCAATCTTGGGAAAAAAATATTTGGATACCAATCCTTACCAAGATATTTCATTACTTTCTCAGTGGAGTAGTATTATTAGAGAGAGTGGGAAAGATGTGGGAGACAATGAAGTTGTAAATATGAAGATTGGAGAAATTAATGAGTTAGAAAACGGATTTTTTAATATCCCGATTAGTATTTCATTTAAATCTGACTCAAAGAGAGCCTTCCTTCTTTTGGTAGATAAGCTTTCTCTGACTTCTAATATCAATAATTTGGGATTGCTCAATGACTTTTCTTACTATCTTTTCAATGTCATTAGAGAGAAAAAGGCTGAAGAAATTAATCAATTGGTCTCAGAATATCAACTTACGAAACCTCAGGATCAGAATGAAAAATCTTTTTATAATGCGGTTATCTCTCGTTATTTGTATAACCGATTGCAGACTCCAGATTCTGAATCTAATATCTTGATTGATGAAGAGGTAGTGGATGAGACGATCAAACAAAGTATTGTTTGTGGAATGACTGAATCTTCAGATATTTGTTATTTCAGATTTAGGGATAAGTATAGGACAATTCCAGGACTTGCCTATGGAATCGGACTTGAAAGTCTTGTGAATAAGCCTCTCTCTCTTAAGAGATTCTTTGCGAGTATCCCTCCTCTTATTGCGATCGAATCCTTTACCTTTGATAAGGCAAAAGCACAAGGAGTATCGCTAGCAAACAATAATGAATATCTCGGAAATGTGAATTTTAATATTTATGGTAGAGGGATTTCTGCAGAAGATACGGAACAGATTTCTCGCTTGTTAACTGAGAGTTGTTTTGGAGAGAAGTCTTCTCAAGCTTTAGATGCGAGTTGA", "aa": "MQKGKNGSKLEISFGINKQTYLVTLGIFGVMSVVTVMALFFIWSKNSELTARTWDLKELNKYDLTSFQQSEVMAEDKDSLPQTIEDIVGLYSSSKEEKERIEGELTFKKSVYTEFLRNLLLPSLNIWKDPYTKDIDISILGKKYLDTNPYQDISLLSQWSSIIRESGKDVGDNEVVNMKIGEINELENGFFNIPISISFKSDSKRAFLLLVDKLSLTSNINNLGLLNDFSYYLFNVIREKKAEEINQLVSEYQLTKPQDQNEKSFYNAVISRYLYNRLQTPDSESNILIDEEVVDETIKQSIVCGMTESSDICYFRFRDKYRTIPGLAYGIGLESLVNKPLSLKRFFASIPPLIAIESFTFDKAKAQGVSLANNNEYLGNVNFNIYGRGISAEDTEQISRLLTESCFGEKSSQALDAS", "aa_hexdigest": "e5f849bffe3f0be82d05d77ac23b9812", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A660MTQ3", "query_cov": 1.0, "subject_cov": 0.8148148148148148, "identity": 0.998, "score": 803.0, "evalue": 1.37e-291, "valid": true, "uniref50_id": "UniRef50_A0A7W4HGX6" }, "pscc": { "uniref50_id": "UniRef50_A0A7W4HGX6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7W4HGX6" ], "product": "PpiC domain-containing protein" }, "genes": [ "ppiC" ], "id": "NKOCPLAKPK_99", "locus": "GCA003260355_00050" }, { "type": "cds", "sequence": "contig_1", "start": 30874, "stop": 31011, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAAATCTTTTGGAGCTTAAGGATACCTTATTGCAAGAGGGTGCTTCATATGACTCTTTGTCTCCTTATAATAAGATGATCAAGAAGTTTGAGTTTTATAGAATGCTCAAAAACATTGGGCTTTGTAGAGGGTAA", "aa": "MANLLELKDTLLQEGASYDSLSPYNKMIKKFEFYRMLKNIGLCRG", "aa_hexdigest": "a65a0810479391f6d2538d003c4ac71e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5244.1368, "isoelectric_point": 8.88891696929932 }, "id": "NKOCPLAKPK_100", "locus": "GCA003260355_00051" }, { "type": "cds", "sequence": "contig_1", "start": 31184, "stop": 31450, "strand": "-", "frame": 3, "gene": null, "product": "CoA-binding domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EFN5", "UniRef:UniRef90_A0A328EFN5" ], "nt": "ATGTCTACCCTTGCCCTGATTGGAGCCTCTGAGAATCCAGAATCCCTTGCGTTTAGGCTCTTTACTCGCCTAAGAGAAGGAGGCTTTGAAGTCGTTCCCATCAACCCAAAATATCAAACGATAGCTGGAGCTCCGTGCTTTGCTTCTCTTTCCGAGCTAAAAACTACTCCCGAAGTCGTGATTTTTATGGTCAATCCGAAACTGACTTTACAAATCCTTCCTCAAGTCGTAGAACTAAAAATCAAAAAAGTCTGGTTTCAGCCTTGA", "aa": "MSTLALIGASENPESLAFRLFTRLREGGFEVVPINPKYQTIAGAPCFASLSELKTTPEVVIFMVNPKLTLQILPQVVELKIKKVWFQP", "aa_hexdigest": "e6d0f50ed01fa4571f65ec55fefb3ca4", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 31073, "stop": 31450, "observations": { "mutation": [ 31186 ], "stop": [ 31186 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EFN5", "query_cov": 1.0, "subject_cov": 0.6929133858267716, "identity": 0.932, "score": 162.0, "evalue": 4.93e-50, "gene_start": 1, "gene_end": 88, "reference_start": 1, "reference_end": 88, "reference_sequence": "MSSLALIGASENPESLAFKLFTRLREGGFEVIPINPKYQTIAGVTCFASLSELKTTPEVVIFMVNPKLTLQILPQVVELQIKKVWFQPGTFDDAVLEFCKQNNLKVEHEKCLLIAPITTLETFIQGE" }, "paralog": false, "identity": 0.936, "score": 235.0, "evalue": 2.68e-81, "description": "Internal stop codon at 31186. Nonsense mutation around 31186.", "psc": { "uniref90_id": "UniRef90_A0A328EFN5", "product": "CoA-binding domain-containing protein", "uniref50_id": "UniRef50_A0A328EFN5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EFN5", "UniRef:UniRef50_A0A328EFN5" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EFN5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EFN5" ], "product": "CoA-binding domain-containing protein" } }, "id": "NKOCPLAKPK_101", "locus": "GCA003260355_00052" }, { "type": "cds", "sequence": "contig_1", "start": 31501, "stop": 31668, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAGGTAGAAAAATTATTTACTATTTCTTATCTCATGATTCAACATTATTTCTTTAACGCACTTGAGAATCCTCAGACACTTAGTCTCTTTGGGAGAGTATTAGAATCTTTGTTTTGGAGAGCTTTTTCCCTCGTGGCATGCAGTATTTTTTTACTTTTTGTTTGA", "aa": "MEVEKLFTISYLMIQHYFFNALENPQTLSLFGRVLESLFWRAFSLVACSIFLLFV", "aa_hexdigest": "2f2d4c0566a2ee368a30579b81b141f7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6524.666799999997, "isoelectric_point": 5.548872566223144 }, "id": "NKOCPLAKPK_102", "locus": "GCA003260355_00053" }, { "type": "cds", "sequence": "contig_1", "start": 31816, "stop": 32037, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCTTCAACTCCTAAGTTGGAGTTTTGTTCGTTTTATCTTTGATGCAATGTCTCAAGGACTAGAAACCTCAAATACAAATGGACATTTTGCTATCAATATGCTTTTGGAGAAATGGGCATCTTTCTCTTTGTTGATCTTTATGATCGCCCCTTATTTTATCACGTTTTCCTTTGGGAATAAGCTCGTTCGTAATTTAGGGATACTGACCTATATTTTTTGA", "aa": "MLQLLSWSFVRFIFDAMSQGLETSNTNGHFAINMLLEKWASFSLLIFMIAPYFITFSFGNKLVRNLGILTYIF", "aa_hexdigest": "6b116c4fdbe82874e194e36ac4e37a36", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8477.933099999993, "isoelectric_point": 8.210709571838382 }, "id": "NKOCPLAKPK_103", "locus": "GCA003260355_00054" }, { "type": "cds", "sequence": "contig_1", "start": 32134, "stop": 32715, "strand": "-", "frame": 1, "gene": null, "product": "ATP-dependent Clp protease proteolytic subunit", "db_xrefs": [ "EC:3.4.21.92", "KEGG:K01358", "SO:0001217", "UniRef:UniRef50_P43867", "UniRef:UniRef90_A0A2W4IH61" ], "nt": "ATGACCCTGATTCCTACCGTCCTTGAAAAAACCAAAAATGGAGAAAGAGCCTACGATATTTACTCAAGACTCCTTGAAGATAGAATTATTTTTGTTGGAGAGCAGGTGCATAGCGCAATGGTGAATACAATCGTAGCTCAAATGCTCTATCTAGAAAAAAAAGATCCTGAGAAAGATATCATCATGTATATCAATACGCCTGGTGGAGAAGTCTATTCAGGACTGGCAATTTACGATACGATGCAGATCCTCAAGTGTGATATTCAAACTATTTGTACCGGACTTGCGGCTTCTATGGGATCGCTTTTGCTTGCTGGAGGAACGAAAGGGAAAAGGTTTGCTCTGCCACATTCTAGGATTATGATTCATCAGCCTTTGATTTCTGGAGGAGGAATTTCTGGACAGGCGACCGATATTCAGATTGAAGCTGAAGAGATGCTCAAAGTAAAAAAAATGCTGACTGAAATCATCGCGAAGCATTCAGGGAAAGGCTATGATGAAGCGCTTGCTGATATGGAGAGAAATAAGTGGATGAGCCCAGAAGATGCTTTGAATTACGGTTTGATTGATAAGATTATCTGA", "aa": "MTLIPTVLEKTKNGERAYDIYSRLLEDRIIFVGEQVHSAMVNTIVAQMLYLEKKDPEKDIIMYINTPGGEVYSGLAIYDTMQILKCDIQTICTGLAASMGSLLLAGGTKGKRFALPHSRIMIHQPLISGGGISGQATDIQIEAEEMLKVKKMLTEIIAKHSGKGYDEALADMERNKWMSPEDALNYGLIDKII", "aa_hexdigest": "d4e97eefef5cbe5f3decd70c8f88506b", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A2W4IH61", "query_cov": 0.9896373056994818, "subject_cov": 0.9455445544554455, "identity": 0.995, "score": 378.0, "evalue": 1.39e-132, "valid": true, "product": "ATP-dependent Clp protease proteolytic subunit", "ec_ids": [ "3.4.21.92" ], "uniref50_id": "UniRef50_P43867", "kegg_orthology_id": "K01358" }, "pscc": { "uniref50_id": "UniRef50_P43867", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P43867" ], "product": "ATP-dependent Clp protease proteolytic subunit" }, "genes": [], "id": "NKOCPLAKPK_104", "locus": "GCA003260355_00055" }, { "type": "cds", "sequence": "contig_1", "start": 33010, "stop": 33879, "strand": "+", "frame": 1, "gene": null, "product": "Peptidoglycan binding-like domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EJ24", "UniRef:UniRef90_A0A328EJ24" ], "nt": "ATGGGAGCTACTCTTTTACTCTTGAGCCCGTCGATTTTGGCGGCGAACGTTAGCCAAACTCAGGTGAAAGAATTTTCAACGAAGTTGGAAAATCATAAAAATAAGCTTCTTAAAACTCTTGATGAGAGTAAGGTAAAACTCAAAAAGGTTTCAACTGAAGCTGCAAAGTTTGCGGATACGAGTCTGTTTAGAGCGGCAAGTTGTTTGGGGGCGATTCCTGCTGAGGATCAGAATTTAAACTTTGATAAACTTGCTGATGCTTTGAAAACTTCTATTCTTAATGAATATATTAAGTTTGATGGGGATATTAAGAGATTAGGATTTGGAATGGCGGAGCAGGATCCTTTGATTTTTGGGAATACTCTTGATACTTTTTATAATCAGCAAGCGCTGAAAATTAGCTCTCTTGAGAGTGATTATTATCTCAAGACTGAAAAAGTGAAGAAAACTTTTCTTGAGTATGTAGAAAATAATAAGACTTTGCTTACAAGTTTGGCACAAAATCTTGATTATTTGGATGCTTTTCAAAAGACTGTGAGTGGAGTGACCCTTGCCTTTAATGAATTTAAGGCTACGGTAGATAGTCGTTCTGATCTTTTTAAAGCAATAGAAAAGGAAAAGTCTGTGCTTGAGAAGAGTTTTTCTGCTGAATTAGAGTCAATCTTTAATCAAGCACTTGTAAAGAATAAGCCAGATGATGCGACTCAGGCTAAGTATTTGATCCATAAAGATAATTTTTTGAAGAAGTTTACAACTGATATTCAACAGTCAGAATATTATCTTTTTTCTGCTTTGTTTTCTTATGCTGATTATGCAGATATTATGGGGAAAAAATCAGATCTTGAGAAAAAATTCTACTCTGCTTCTTGA", "aa": "MGATLLLLSPSILAANVSQTQVKEFSTKLENHKNKLLKTLDESKVKLKKVSTEAAKFADTSLFRAASCLGAIPAEDQNLNFDKLADALKTSILNEYIKFDGDIKRLGFGMAEQDPLIFGNTLDTFYNQQALKISSLESDYYLKTEKVKKTFLEYVENNKTLLTSLAQNLDYLDAFQKTVSGVTLAFNEFKATVDSRSDLFKAIEKEKSVLEKSFSAELESIFNQALVKNKPDDATQAKYLIHKDNFLKKFTTDIQQSEYYLFSALFSYADYADIMGKKSDLEKKFYSAS", "aa_hexdigest": "6e97b4cffcfa665f24d2c88bb28c98fa", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 32986, "stop": 34182, "observations": { "mutation": [ 32998, 33877, 34138 ], "stop": [ 32998, 33877, 34138 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EJ24", "query_cov": 1.0, "subject_cov": 0.5971074380165289, "identity": 1.0, "score": 541.0, "evalue": 4.18e-191, "gene_start": 1, "gene_end": 289, "reference_start": 9, "reference_end": 297, "reference_sequence": "MKKIGFLLMGATLLLLSPSILAANVSQTQVKEFSTKLENHKNKLLKTLDESKVKLKKVSTEAAKFADTSLFRAASCLGAIPAEDQNLNFDKLADALKTSILNEYIKFDGDIKRLGFGMAEQDPLIFGNTLDTFYNQQALKISSLESDYYLKTEKVKKTFLEYVENNKTLLTSLAQNLDYLDAFQKTVSGVTLAFNEFKATVDSRSDLFKAIEKEKSVLEKSFSAELESIFNQALVKNKPDDATQAKYLIHKDNFLKKFTTDIQQSEYYLFSALFSYADYADIMGKKSDLEKKFYSASGAVNCNLLLTTSANIGGYIDGTEAKAEQLKRGLKTLTDAIKNGRLNLKMLEEPALSYFKDTNAQMAKKLQANFRAMLDSETPQSMFTGNLSQTGQQVSSEGALPVQKVTFTQSFNKGQYHEQIKTLQTVLKNRGYYHSVIDGVYSPATIEAVYQFQLKEGIVTGKEKNKAGHGWFGQKTREKLNSLL" }, "paralog": false, "identity": 0.992462311557789, "score": 756.0, "evalue": 4.82e-278, "description": "Internal stop codon at 32998, 33877, 34138. Nonsense mutation around 32998, 33877, 34138.", "psc": { "uniref90_id": "UniRef90_A0A328EJ24", "product": "Peptidoglycan binding-like domain-containing protein", "uniref50_id": "UniRef50_A0A328EJ24", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EJ24", "UniRef:UniRef50_A0A328EJ24" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EJ24", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EJ24" ], "product": "Peptidoglycan binding-like domain-containing protein" } }, "id": "NKOCPLAKPK_105", "locus": "GCA003260355_00056" }, { "type": "cds", "sequence": "contig_1", "start": 33898, "stop": 34140, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTAACGACAAGTGCTAATATTGGGGGATATATTGATGGCACAGAGGCAAAGGCAGAGCAGTTGAAGAGAGGCTTGAAGACTCTTACGGATGCGATAAAAAATGGAAGATTAAATCTAAAAATGCTTGAAGAACCTGCACTTTCTTACTTTAAAGATACAAATGCGCAGATGGCGAAAAAGTTGCAGGCTAATTTTAGGGCTATGCTTGATTCAGAAACTCCTCAGTCTATGTTCACTTGA", "aa": "MLTTSANIGGYIDGTEAKAEQLKRGLKTLTDAIKNGRLNLKMLEEPALSYFKDTNAQMAKKLQANFRAMLDSETPQSMFT", "aa_hexdigest": "785d3e2129390356d7009399715c013c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8872.125199999999, "isoelectric_point": 9.048476219177246 }, "id": "NKOCPLAKPK_106", "locus": "GCA003260355_00057" }, { "type": "cds", "sequence": "contig_1", "start": 34183, "stop": 34440, "strand": "+", "frame": 1, "gene": null, "product": "Peptidoglycan-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MUU2", "UniRef:UniRef90_A0A660MUU2" ], "nt": "TTGCCTGTGCAAAAGGTGACTTTTACTCAGTCGTTCAATAAGGGACAATATCATGAGCAGATAAAAACTCTTCAGACGGTTCTTAAGAATCGAGGATATTATCACTCTGTTATTGATGGGGTATACTCTCCTGCTACTATTGAAGCGGTATATCAGTTTCAGCTCAAAGAGGGTATTGTGACAGGAAAGGAGAAAAATAAGGCTGGTCATGGATGGTTTGGGCAAAAAACGAGGGAGAAGCTAAATAGTCTTTTGTAG", "aa": "MPVQKVTFTQSFNKGQYHEQIKTLQTVLKNRGYYHSVIDGVYSPATIEAVYQFQLKEGIVTGKEKNKAGHGWFGQKTREKLNSLL", "aa_hexdigest": "a67c9d4213af1119a7d04b8aabbcc0cc", "start_type": "TTG", "rbs_motif": "TTCTT", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 34069, "stop": 34440, "observations": { "mutation": [ 34138 ], "stop": [ 34138 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A660MUU2", "query_cov": 1.0, "subject_cov": 0.6910569105691057, "identity": 0.976, "score": 172.0, "evalue": 5.93e-54, "gene_start": 1, "gene_end": 85, "reference_start": 39, "reference_end": 123, "reference_sequence": "MAKKLQANFRAMLDSETPQSTFTGNLSQTGQQVSSEGALPVQKVTFTQSFNKGQYHEQIKTLQTVLKNRGYYHSAIDGVYSPATIEAVYQFQLKEGIVTGKEKNKAGHGWFGQKTREKLNSLL" }, "paralog": false, "identity": 0.975609756097561, "score": 240.0, "evalue": 2.25e-83, "description": "Internal stop codon at 34138. Nonsense mutation around 34138.", "psc": { "uniref90_id": "UniRef90_A0A660MUU2", "product": "Peptidoglycan-binding protein", "uniref50_id": "UniRef50_A0A660MUU2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A660MUU2", "UniRef:UniRef50_A0A660MUU2" ] }, "pscc": { "uniref50_id": "UniRef50_A0A660MUU2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MUU2" ], "product": "Peptidoglycan-binding protein" } }, "id": "NKOCPLAKPK_107", "locus": "GCA003260355_00058" }, { "type": "cds", "sequence": "contig_1", "start": 34481, "stop": 35083, "strand": "+", "frame": 2, "gene": "gspG", "product": "Type II secretion system protein GspG C-terminal domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EFH1", "UniRef:UniRef90_A0A328EFH1" ], "nt": "ATGATGAAAAAGAGAGGATTTACCTTGCTTGAGGTGACGCTTGTGATCGTAATTTCTTCGTCTATTTTGACGACACTTTATGGAATTATGACGCTTTTGCCCAAAGTAAAGAATACGAATGATGCGAGGCAAAATCTGATTCAACAAACGAACGATGTTATAGATAGTTTCGCGAGAATCTTTCAGGATTATACTATTGATTATGAGGAATATTTTAATAGGCAAATGGTCGGATGCAATAGTAACAAAAGAGGAGAGAATTTTCAGTGGAGCTTAAATGCAAGTGGGCACTGTAATGAATTTACGGCTTATGGAAATGAAAACTCTTCCAGAGTGCAAACTGATGAACTGAAGCAGCATTTCCTTTCTTATTGTTCTTCAAATGGAGTACAGTGGCTTTTGAATTTTGTGAATAATTGTCAATCTGCGAATCTTCCTAATGATCGTAATCTGGGAAGGCAGTCTTATGGGCAATATAAGTGGCTCTTTTGGGATATTAGAGGAGATACGGATGGGCAGTCGATTAATGGAGTTACGGAGCTTAACCTTTCAGAAAAATATAAACCGCTAGAATTTGGGGATTCTGACGATAGGGATGAGTGA", "aa": "MMKKRGFTLLEVTLVIVISSSILTTLYGIMTLLPKVKNTNDARQNLIQQTNDVIDSFARIFQDYTIDYEEYFNRQMVGCNSNKRGENFQWSLNASGHCNEFTAYGNENSSRVQTDELKQHFLSYCSSNGVQWLLNFVNNCQSANLPNDRNLGRQSYGQYKWLFWDIRGDTDGQSINGVTELNLSEKYKPLEFGDSDDRDE", "aa_hexdigest": "1d3d8ba0cd444b9f7428c1338def4d7a", "start_type": "ATG", "rbs_motif": null, "genes": [ "gspG" ], "truncated": "3-prime", "pseudogene": { "start": 34481, "stop": 35383, "observations": { "mutation": [ 35081, 35087, 35102 ], "stop": [ 35081, 35087, 35102 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EFH1", "query_cov": 1.0, "subject_cov": 0.5128205128205128, "identity": 0.97, "score": 392.0, "evalue": 4.33e-135, "gene_start": 1, "gene_end": 200, "reference_start": 1, "reference_end": 200, "reference_sequence": "MMKKRGFTLLEVTLVIVISSSILTTLYGIMTLLPKVKNTNDARQNLIQQTNDVIDSFARIFQDYTIDYEEYFNRKMVGCNSNKRGGNFQWSLNASGHCNEFTAYGNENSSRVQTDELKQHFLSYCSSNGVQWLLNFANNCQSANLPNDRNLGRQSYGQYKWLFWDIRGDTDGQTINGVTELSLVEKYKPLEFGDSDDRDEGTGPDAVGDSNGVQELYLISHDKTRRLFLRRTLKEYKDRNGVVSTGYTIQILKLRGFDAGRKHAFKAEEDLEVYDGQVDTWACDYGQKFSCKGDDIPNYAGYKLPKDKDDGWVDLFDDNLNIVHWNIQISPVRDPDFARAIDDGQINPFIKMSLTAQLNQHLWANKVGGDTKNFQYTLQNVYDTKGFYLR" }, "paralog": false, "identity": 0.9666666666666667, "score": 597.0, "evalue": 2.37e-218, "description": "Internal stop codon at 35081, 35087, 35102. Nonsense mutation around 35081, 35087, 35102.", "psc": { "uniref90_id": "UniRef90_A0A328EFH1", "product": "Type II secretion system protein GspG C-terminal domain-containing protein", "uniref50_id": "UniRef50_A0A328EFH1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EFH1", "UniRef:UniRef50_A0A328EFH1" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EFH1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EFH1" ], "product": "Type II secretion system protein GspG C-terminal domain-containing protein" } }, "id": "NKOCPLAKPK_108", "locus": "GCA003260355_00059" }, { "type": "cds", "sequence": "contig_1", "start": 35132, "stop": 35383, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTTCTCATGATAAGACAAGAAGGCTCTTTTTAAGAAGGACTTTGAAAGAATATAAGGATCGTAATGGCGTGGTTTCGACTGGATATACTATTCAGATTTTGAAATTAAGGGGCTTCGATGCTGGAAGAAAGCATGCGTTTAAGGCGGAAGAAGATTTAGAAGTTTATGATGGACAGGTGGATACTTGGGCGTGTGATTATGGACAAAAGTTTTCTTGTAAGGGAGATGATATTCCGAATTATGCATGA", "aa": "MISHDKTRRLFLRRTLKEYKDRNGVVSTGYTIQILKLRGFDAGRKHAFKAEEDLEVYDGQVDTWACDYGQKFSCKGDDIPNYA", "aa_hexdigest": "054c74980e076cbdcec1a6cc1f699b5f", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9650.775699999995, "isoelectric_point": 8.580693626403807 }, "id": "NKOCPLAKPK_109", "locus": "GCA003260355_00060" }, { "type": "cds", "sequence": "contig_1", "start": 35839, "stop": 36222, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGTTAAGCATGATGAATATGAAAGACAGCTCAGTAAATTGCACTATAGAGAGTCGCTCCTTGATCCTGAAAGGGGGAATATTTTTGCATTGGATAAGGGAGGGCATCCTGTGAAATTAACCGAAAATTTGACACTTTATGATCTGGCACTTGATCCTAATGATTTGACAGTTTTCCCGGAAAAAAAGCAAGATGGGCACGTTATTTCGCCAGAGACCCCTATGAAGCCGCGTTTTATTGAATTAGTTGCTCCAGTAATTTACAAGCATTTGTGTGAGTTAAATGGGATGAAAGTCCCAACCAAGGCTGAATGTGTCAAAAATGTAGAACTCTTTACGAATGTGGATTTACTTCCAAAACAGCCAGAATTATTTTATTTTTGA", "aa": "MVKHDEYERQLSKLHYRESLLDPERGNIFALDKGGHPVKLTENLTLYDLALDPNDLTVFPEKKQDGHVISPETPMKPRFIELVAPVIYKHLCELNGMKVPTKAECVKNVELFTNVDLLPKQPELFYF", "aa_hexdigest": "ebc49fbe2cb5560e8a9fcd6d5c07bc3c", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14690.842999999988, "isoelectric_point": 5.711261558532715 }, "id": "NKOCPLAKPK_110", "locus": "GCA003260355_00061" }, { "type": "cds", "sequence": "contig_1", "start": 36319, "stop": 36615, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAGCTAATTACCACCAGGCTTGATGAAAAAATTCAGATAGGAAAAAAAACCTCAAACTATCTAGGGTATTTTGTTGAACCTAGGTTTTTGGAGGAGGTGAAGCAATTGAATCTGCCTTATCTTACTATTGAAAAGGAGAATTATCTGTATATTGTGCCACTGAATGTGCAGAATAAAACGCAGGCGATTAATCAGGTGAAATCTTTACTGAAGAAATGGAGATATCCGATAAGTTCAACTTTTGATAGTCTGTTTGAAGTGCAGGATTGGAGATATATTAAGCTCATTTCTTGA", "aa": "MELITTRLDEKIQIGKKTSNYLGYFVEPRFLEEVKQLNLPYLTIEKENYLYIVPLNVQNKTQAINQVKSLLKKWRYPISSTFDSLFEVQDWRYIKLIS", "aa_hexdigest": "fc7152822b3df135f25c94e3fe9df7ae", "start_type": "ATG", "rbs_motif": "TTTA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11777.55119999999, "isoelectric_point": 9.125515937805176 }, "id": "NKOCPLAKPK_111", "locus": "GCA003260355_00062" }, { "type": "cds", "sequence": "contig_1", "start": 36961, "stop": 37293, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGACGCTTGATATTGGGCTTCAGAGAGAAGCAGAACAAATTGCTCATGAGCAACTTCAGCAATTGAGAGCAGATGCGATTAGTATCCTAGTTTTGGATGCTGAGAATGGGCAAGTTAAAGCTTCAGTTAATGCTCCGACTTTTAATCCTAATAATTATAATGATGCTTATACCTTAATGCCATTAGGACAGGAATATGCGCATTTGATTGATAATTTGAGCTATGTTGATGTGCCGGTTTATATTTTTACGGGAAACGATTATAGGATTGCTACCTTATCCGAAAGGCAGAATACTGGACTTCAAAAATTTATTAACACGAATGTTTTTTGA", "aa": "MTLDIGLQREAEQIAHEQLQQLRADAISILVLDAENGQVKASVNAPTFNPNNYNDAYTLMPLGQEYAHLIDNLSYVDVPVYIFTGNDYRIATLSERQNTGLQKFINTNVF", "aa_hexdigest": "362babe0d3233d03f1f1cf2c4a04e395", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12389.69669999999, "isoelectric_point": 4.456313514709472 }, "id": "NKOCPLAKPK_112", "locus": "GCA003260355_00063" }, { "type": "cds", "sequence": "contig_1", "start": 37522, "stop": 37692, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00905.27" ], "nt": "ATGGTGAAGATTGTGCAGAGAATACAAAAATATGCCTTTTATAATTATCTTACGAAACTTGGTTTTGGATCATTTACTGAAATTGAACTTGCTGGAGAGAAGGAGGGCTTCTTGGATAATGTAAATCTCGTTTCTGTGGCAAGATTTTTGAATAATGCTTTTGGTCAGTGA", "aa": "MVKIVQRIQKYAFYNYLTKLGFGSFTEIELAGEKEGFLDNVNLVSVARFLNNAFGQ", "aa_hexdigest": "13013c00aad1fa1658b6431736d96c9e", "start_type": "ATG", "rbs_motif": "TTCTT", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00905.27", "name": "Transpeptidase", "length": 45, "aa_cov": 0.8035714285714286, "hmm_cov": 0.9555555555555556, "evalue": 1.0691492597580571e-06, "score": 24.545473098754883, "start": 1, "stop": 45 } ], "seq_stats": { "molecular_weight": 6393.2822000000015, "isoelectric_point": 8.064366340637207 }, "id": "NKOCPLAKPK_113", "locus": "GCA003260355_00064" }, { "type": "cds", "sequence": "contig_1", "start": 37699, "stop": 37881, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00905.27" ], "nt": "GTGACTCAGATTCAGCTTGCAGTTGCCTATGCTTCTTTAGTAAATGGAGGAGAGCGTATCAAACCAACTATTATTTCCAAAATTATTAATAAAGAACAAAATGGCTTGGAACAAGAAGTTTCTTTGATACAGGAGAGAAAGCAGATTTTTAAGCATAAAACTTCAGAGCTCTTGAGGTCTTGA", "aa": "MTQIQLAVAYASLVNGGERIKPTIISKIINKEQNGLEQEVSLIQERKQIFKHKTSELLRS", "aa_hexdigest": "9bfbf1c56bd0002fcda1815f4280341b", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00905.27", "name": "Transpeptidase", "length": 59, "aa_cov": 0.9833333333333333, "hmm_cov": 0.864406779661017, "evalue": 1.2988771046295582e-07, "score": 27.55120277404785, "start": 1, "stop": 59 } ], "seq_stats": { "molecular_weight": 6821.855899999999, "isoelectric_point": 9.599294090270995 }, "id": "NKOCPLAKPK_114", "locus": "GCA003260355_00065" }, { "type": "cds", "sequence": "contig_1", "start": 38188, "stop": 38370, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATACTGTTAACCTTTCAAAATTAGTATTGAGTTCTTATTTCTTTGATCTTTTGGCTCAGGAAAATATCCATTTTGAGCAGATTTTTAGTGATAATCCTGCTTTGGCTGCTTTTGGGGTTTCGGTGATTGATAGAGATTTGAGCACTTTTTTTGAGAGGCAATCAAAGAAAAAAAACTGA", "aa": "MNTVNLSKLVLSSYFFDLLAQENIHFEQIFSDNPALAAFGVSVIDRDLSTFFERQSKKKN", "aa_hexdigest": "982adb9ab80b16ee566ee802685750a6", "start_type": "ATG", "rbs_motif": "TTATT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6885.720200000002, "isoelectric_point": 5.587409400939941 }, "id": "NKOCPLAKPK_115", "locus": "GCA003260355_00066" }, { "type": "cds", "sequence": "contig_1", "start": 38380, "stop": 38817, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MQ78", "UniRef:UniRef90_A0A660MQ78" ], "nt": "TTGGATCAGCTTTCTTTTTCTGATATTACACCTTTTCTTGGGCAACTTTTTGAAGATGTTATTGTGATTGATGCGTGGACGGGAGTGTCTAGTTTTTGTAGAAAATTTGTTCCCGAATTACGTTTTTTGGAGGGGGCAGAGGACGTCGTATTTCCCCTTGATCTTCAGTCTTTTTTACGTGCTTTAACAACATGTAAATCGGCTTTAATCCCTCTTACTGCTCAAGAAATTCCCGAAAATATTTATGAATCTCCTTCTGAAGAGGTTTTTCAGATTGTAGATAAACACTTGGTTGATCATTCTGATTTTCTCCCTTTAGCTACTCCTGATCATCCGGAGCTTCTTCTTATCGGTATGGGAAGTTATTTTGAGGAGTATGTAAAACTTTCTCAACTTTTGGATGCTTGCCCAGAACGTATTGCATTGGGTGTTTTGTGA", "aa": "MDQLSFSDITPFLGQLFEDVIVIDAWTGVSSFCRKFVPELRFLEGAEDVVFPLDLQSFLRALTTCKSALIPLTAQEIPENIYESPSEEVFQIVDKHLVDHSDFLPLATPDHPELLLIGMGSYFEEYVKLSQLLDACPERIALGVL", "aa_hexdigest": "649c43ed9f0fb5041c3757a66488ee2e", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 38188, "stop": 39066, "observations": { "mutation": [ 38368, 38815 ], "stop": [ 38368, 38815 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A660MQ78", "query_cov": 1.0, "subject_cov": 0.4965753424657534, "identity": 0.897, "score": 261.0, "evalue": 8.2e-86, "gene_start": 1, "gene_end": 145, "reference_start": 65, "reference_end": 209, "reference_sequence": "MNTVNLSKLVLSSYFFDLLAQENIHFEQIFSDNPALAAFGVSVIDRDLSTFFESQSKKKNGLIILDQLSFSDITPFLGQLFENVIVIDAWTGMSSFGRKFAPELRFLEGAEDVVFPLDLQTFLRALASHKSALIPLTAQEIPENVYESPSEEVFQIVDKHLVDHSEFLSLTTLEHPELLLIGMGSYFEEYVKLSQLLDACPERIALGVLGKTSALGLDSVQRLVKNAKKIGICIDHESLSALAYFRRSLHDKPIFMITPDYKKLTTFAPEWQLEQVSFDASSLLERSLKLLV" }, "paralog": false, "identity": 0.934931506849315, "score": 530.0, "evalue": 2.34e-194, "description": "Internal stop codon at 38368, 38815. Nonsense mutation around 38368, 38815.", "psc": { "uniref90_id": "UniRef90_A0A660MQ78", "uniref50_id": "UniRef50_A0A660MQ78", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A660MQ78", "UniRef:UniRef50_A0A660MQ78" ] }, "pscc": { "uniref50_id": "UniRef50_A0A660MQ78", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MQ78" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 16305.531699999989, "isoelectric_point": 4.200935935974121 }, "id": "NKOCPLAKPK_116", "locus": "GCA003260355_00067" }, { "type": "cds", "sequence": "contig_1", "start": 38845, "stop": 39066, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCGGCGTTTGGTAAAAAATGCAAAAAAGATTGGAATCTGCATTGATCACGAATCTCTATCGGCACTTGCCTATTTTAGGAGATCTTTGCATGATAAGCCAATCTTTATGATCACTCCTGACTATAAGAAACTTACAACTTTTGCTCCGGAATGGCAACTCGAGCAGGTGAGTTTTGATGCTTCCTCATTATTGGAAAGGAGTTTGGAATTGCTGGTTTAA", "aa": "MRRLVKNAKKIGICIDHESLSALAYFRRSLHDKPIFMITPDYKKLTTFAPEWQLEQVSFDASSLLERSLELLV", "aa_hexdigest": "31c87e40289bfe1f6b5f791846cb0c05", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8509.876999999999, "isoelectric_point": 8.889045906066897 }, "id": "NKOCPLAKPK_117", "locus": "GCA003260355_00068" }, { "type": "cds", "sequence": "contig_1", "start": 39072, "stop": 39227, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCATTTGCCTATGCCAACGAAAAAGGATGCGGAGTCCCAAATGTGATCCATGCATCAGGAGACAGTGAGGAAGCAGAAAAAGAAATCAGACATCGATTTAACGATTCAGAACTCTTCAACTACAGAAGAGCAGATGCAGACTTCATAAATTAA", "aa": "MSFAYANEKGCGVPNVIHASGDSEEAEKEIRHRFNDSELFNYRRADADFIN", "aa_hexdigest": "a57aabe477916a91697b50b449c6e595", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5822.246499999998, "isoelectric_point": 4.887323570251465 }, "id": "NKOCPLAKPK_118", "locus": "GCA003260355_00069" }, { "type": "cds", "sequence": "contig_1", "start": 39282, "stop": 39572, "strand": "-", "frame": 2, "gene": null, "product": "nucleoside-diphosphate kinase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0G1NQQ1" ], "nt": "ATGAACACAGAAAGGACACTCGTAGTCTTCAAACCTGATGCAGTTTGTCGCGGGATCGTAGGTCAAATCCTAACCCGCTTTGAACAGGTTGGACTCAAAATCGTAGGAATAAAGATGAAAAAACCTGACAGAGATTTCCTCCATCATCATTACGAAACAATTGGAACTATGATCACTCGTCGTGGAGAAGAGACCTTCAACCTTAACCTAGAAGCCATGCAAAAAAGCCCTGTTATAGCGATGGTAGTGGAAGGGGTCGAAGCAGTAAGCCTCGTAAGGAAAATGGCATGA", "aa": "MNTERTLVVFKPDAVCRGIVGQILTRFEQVGLKIVGIKMKKPDRDFLHHHYETIGTMITRRGEETFNLNLEAMQKSPVIAMVVEGVEAVSLVRKMA", "aa_hexdigest": "5cda4ed518321981eaaa7a54adbc72e5", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A0G1NQQ1", "query_cov": 0.96875, "subject_cov": 0.9029126213592233, "identity": 0.516, "score": 81.3, "evalue": 4.25e-18, "valid": false, "product": "nucleoside-diphosphate kinase", "uniref50_id": "UniRef50_A0A0G1NQQ1" }, "pscc": { "uniref50_id": "UniRef50_A0A0G1NQQ1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0G1NQQ1" ], "product": "nucleoside-diphosphate kinase" }, "genes": [], "id": "NKOCPLAKPK_119", "locus": "GCA003260355_00070" }, { "type": "cds", "sequence": "contig_1", "start": 39799, "stop": 39951, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGATAAGCCGGGTAGTGATCTCAAAAATACGAGAAAACCAGTTCCTACCTTACAAGGAATATTTGTGATTCTTGCCCTCGTGGCGGGAATTGGCGTTATTTATCCTACTTATTTTTCTGAGCCGCTTTTTCTCGGGCTTCTGATTCCATGA", "aa": "MDKPGSDLKNTRKPVPTLQGIFVILALVAGIGVIYPTYFSEPLFLGLLIP", "aa_hexdigest": "5713e3a7034cfca80e56bbee37e3153f", "start_type": "TTG", "rbs_motif": "TTTA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5414.444899999998, "isoelectric_point": 8.139085197448733 }, "id": "NKOCPLAKPK_120", "locus": "GCA003260355_00071" }, { "type": "cds", "sequence": "contig_1", "start": 39964, "stop": 40107, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGTAGAGTTGATAGAAGAGCTTAGCTATCTTGGGAAATTTCCAAAGATTCCTCCGATAGTAAGGCTTGTCGTTCATGTGTTGGCTGCATTTTTGGCGGTGTGGATCGGTGGTTTGAATCAGCATTCTGAATTTGTTTTGTGA", "aa": "MVELIEELSYLGKFPKIPPIVRLVVHVLAAFLAVWIGGLNQHSEFVL", "aa_hexdigest": "a11f57c3a0b28ed24fd7e672df05b72c", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5287.308499999998, "isoelectric_point": 5.999321556091308 }, "id": "NKOCPLAKPK_121", "locus": "GCA003260355_00072" }, { "type": "cds", "sequence": "contig_1", "start": 40111, "stop": 40389, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTTTTCCCGATTCCGAGCTGGATTTTTACGCTGGCTTTTGTCGTCTGGACGATGTTTTGTATTAATGCAATTAACTGGTTTGATGGAATTTACGCACAAGGAAGTGGAATTTCAGCGATTGGTTTTTTGACGATTTATCTTGTGATAAAATTTGTAGTTTTCCAGAGTTATGAAACTTTCCCTAATATTGAATCGCTCCTCTTCGTTCAGAATTTATCTTTTTTGCTTTTTATTATTAGTCTGATTTATACGGTGATTGAGTACAAGCCGCTCTGA", "aa": "MVFPIPSWIFTLAFVVWTMFCINAINWFDGIYAQGSGISAIGFLTIYLVIKFVVFQSYETFPNIESLLFVQNLSFLLFIISLIYTVIEYKPL", "aa_hexdigest": "a8e4db9304bcb0b0f856896fca31d3ad", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10648.572099999992, "isoelectric_point": 4.406579399108887 }, "id": "NKOCPLAKPK_122", "locus": "GCA003260355_00073" }, { "type": "cds", "sequence": "contig_1", "start": 40411, "stop": 40731, "strand": "+", "frame": 1, "gene": null, "product": "Undecaprenyl/decaprenyl-phosphate alpha-N-acetylglucosaminyl 1-phosphate transferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MNB5", "UniRef:UniRef90_A0A660MNB5" ], "nt": "ATGTTTTTTGGGTTTGCTATTGCTTATCTTTCTGTTGCTGGAGGAGATAAGATTGGGACTTTGGTAGTGGCGCTTTCTCTTCCTTTGTTTGATGCGGCTTGGGTTGGGCTTTATAGGATTTTTGTGCTTAAAAAAAATCCCCTCAATGGGGACTATACTCACTTTCATCATAGGCTCTTGGGATTAGGATTTTCGAGAGGAGAAGCTAGGGCTTTTATTCGGATATGGTCGCTTATTATGATGGTTCTGATGCTTCTTCAAGGGACGAATAGATTGCATAAGATTGTTATTTTTCTGCTGATGGCGGCGTTGTTTTTTTGA", "aa": "MFFGFAIAYLSVAGGDKIGTLVVALSLPLFDAAWVGLYRIFVLKKNPLNGDYTHFHHRLLGLGFSRGEARAFIRIWSLIMMVLMLLQGTNRLHKIVIFLLMAALFF", "aa_hexdigest": "25799d14515938211447b0876a342480", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 40165, "stop": 40797, "observations": { "mutation": [ 40387, 40405, 40729 ], "stop": [ 40387, 40405, 40729 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A660MNB5", "query_cov": 1.0, "subject_cov": 0.5047619047619047, "identity": 1.0, "score": 206.0, "evalue": 4.54e-66, "gene_start": 1, "gene_end": 106, "reference_start": 83, "reference_end": 188, "reference_sequence": "MFCINAINWFDGIYAQGSGISAIGFLTIYLVIKFVVFQSYETFPNIESLFFVQNLSFLLFIISLIYTVIEYKPLGLVRDVGIMFFGFAIAYLSVAGGDKIGTLVVALSLPLFDAAWVGLYRIFVLKKNPLNGDYTHFHHRLLGLGFSRGEARAFIRIWSLIMMVLMLLQGTNRLHKIVIFLLMAALFFGVNAYLFLYKKLPCGLQIKKER" }, "paralog": false, "identity": 0.9809523809523809, "score": 405.0, "evalue": 5.73e-147, "description": "Internal stop codon at 40387, 40405, 40729. Nonsense mutation around 40387, 40405, 40729.", "psc": { "uniref90_id": "UniRef90_A0A660MNB5", "product": "Undecaprenyl/decaprenyl-phosphate alpha-N-acetylglucosaminyl 1-phosphate transferase", "uniref50_id": "UniRef50_A0A660MNB5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A660MNB5", "UniRef:UniRef50_A0A660MNB5" ] }, "pscc": { "uniref50_id": "UniRef50_A0A660MNB5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MNB5" ], "product": "Undecaprenyl/decaprenyl-phosphate alpha-N-acetylglucosaminyl 1-phosphate transferase" } }, "id": "NKOCPLAKPK_123", "locus": "GCA003260355_00074" }, { "type": "cds", "sequence": "contig_1", "start": 40816, "stop": 40947, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTACTCAATAACGAAGAAAATAGGAAACAGATAGAGCAAATCCTGAGTGAAGAGCTTGGGAAAGCAGTTCATCTAACGATTAGCTTTGAGCATAAGGAAGCCTACTTCGCAAGGAAAATGGGATTATAA", "aa": "MLLNNEENRKQIEQILSEELGKAVHLTISFEHKEAYFARKMGL", "aa_hexdigest": "817c5ec5bb95059e4394853aadd2f314", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5059.775699999999, "isoelectric_point": 6.045872688293457 }, "id": "NKOCPLAKPK_124", "locus": "GCA003260355_00075" }, { "type": "cds", "sequence": "contig_1", "start": 41104, "stop": 41628, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTCAAGATTTCTTGGAGTAGCAGGAGAAACAACGATCAAAAATTTACTTTCAACCATCAAAACAGGAGAAAGAGAAAAGATTTTCTCTCAGCTTGACGAACTCAACCAGCACGGGATCGACCTCGGACAATTCGCAAAACAAGCGATCTCCTACATTGACCAGCATCTGCTTGAGGACACCGATTTCTTGCTCCACTGTTCAGAAATCTTCGGAAACGTACTAAGCACCCTAAGGCGATACCCATACCCACTCATCGCATACAAAATCGCAATCAATAAAGCCCTTGAAGCTGGGAAGGAATCTCAAAGCACAGGTGGAGAAAAACCTACTGAAAACAAAAAAGAAGAAAAAGCTGATCCAAAACCAGAAGTAAACGTTCAACCGACAGCACCAACACCTCAACCAGCAAAAGCAACAGCAGTAGAACAGGCTCCTCAACCGACAGAAACTCCTCTTCCACCGCAAACACCAGCACCAGAAGAGCAAAAACTAGAGACCCCAACTCAAGAAAAAAAAGTATGA", "aa": "MSRFLGVAGETTIKNLLSTIKTGEREKIFSQLDELNQHGIDLGQFAKQAISYIDQHLLEDTDFLLHCSEIFGNVLSTLRRYPYPLIAYKIAINKALEAGKESQSTGGEKPTENKKEEKADPKPEVNVQPTAPTPQPAKATAVEQAPQPTETPLPPQTPAPEEQKLETPTQEKKV", "aa_hexdigest": "5f0a8381f24f04e39c6432b66c4e1bf0", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19232.49419999998, "isoelectric_point": 5.323335456848144 }, "id": "NKOCPLAKPK_125", "locus": "GCA003260355_00076" }, { "type": "cds", "sequence": "contig_1", "start": 41647, "stop": 42063, "strand": "-", "frame": 1, "gene": null, "product": "DNA polymerase III subunit tau", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A380NXE2" ], "nt": "GTGGAGATCGATGCGGCCTCTCATACAGGAGTAGATAACATCAGAGAGGAAATCATTGATAAAGCACTCTATCCACCAACCGCTCTGAAAAAAAAGATCTACGTGATCGACGAAGTGCATATGCTCAGTAAGGGCGCATTCAATGCACTCCTAAAAACCATCGAAGAACCAAAAAACAACGTATGTTTTATCTTTGCTACGACGGAAATCCACAAAGTCCCTGATACCATCATTTCCCGCTGCCAAGTGTTCAATTACCGCAAAGTCCCAAATAAAGCAATGATCAATCACTTGGAAAAGATCTGCCAAGCAGAACAGCTCCACTACCAAGAAAATGCACTTGAGATCATCGCCAACATTTCAGAAGGGTGCGTGAGAGACGCAGTAAAATACGTAGATCAAGTCAGCATTTTATGA", "aa": "MEIDAASHTGVDNIREEIIDKALYPPTALKKKIYVIDEVHMLSKGAFNALLKTIEEPKNNVCFIFATTEIHKVPDTIISRCQVFNYRKVPNKAMINHLEKICQAEQLHYQENALEIIANISEGCVRDAVKYVDQVSIL", "aa_hexdigest": "6fd7977d85cc2b92cbd163c6414dfa93", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A380NXE2", "query_cov": 0.9637681159420289, "subject_cov": 0.9432624113475178, "identity": 0.519, "score": 128.0, "evalue": 1.32e-35, "valid": false, "product": "DNA polymerase III subunit tau", "ec_ids": [ "2.7.7.7" ], "uniref50_id": "UniRef50_A0A380NXE2", "kegg_orthology_id": "K02343" }, "pscc": { "uniref50_id": "UniRef50_A0A380NXE2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A380NXE2" ], "product": "DNA polymerase III subunit tau" }, "genes": [], "id": "NKOCPLAKPK_126", "locus": "GCA003260355_00077" }, { "type": "cds", "sequence": "contig_1", "start": 42199, "stop": 42327, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGCCTCGCAAATAAATACCGCCCAAAGACCTTTGATGGAATGATCGGACAGGAACATATCACCAATATCCTCAAAGCACAAATGGAAGTGAGAGATAACATCCACCACAATTATCTCCTTTTTTGA", "aa": "MSLANKYRPKTFDGMIGQEHITNILKAQMEVRDNIHHNYLLF", "aa_hexdigest": "74be29e137699885df1612247e13b1f6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4988.725599999999, "isoelectric_point": 8.198589515686034 }, "id": "NKOCPLAKPK_127", "locus": "GCA003260355_00078" }, { "type": "tRNA", "sequence": "contig_1", "start": 42585, "stop": 42658, "strand": "+", "gene": null, "product": "tRNA-Xxx", "score": 62.2, "nt": "GCGGATATGATGTAGTTGGTGTAGCATAGCAGACTTCAGCTCTGTTTGTACGGGTTCGAGTCCCGTTATCCGCT", "db_xrefs": [], "id": "NKOCPLAKPK_1", "locus": "GCA003260355_00079" }, { "type": "tRNA", "sequence": "contig_1", "start": 43189, "stop": 43261, "strand": "+", "gene": "trnA", "product": "tRNA-Ala(tgc)", "amino_acid": "Ala", "anti_codon": "tgc", "score": 76.7, "nt": "GGGCTCTTAGCTTAGTTGGTAGAGCGCCTGCTTTGCACGCAGGAGGTCAGGGGTTCGACTCCCCTAGAGTCCA", "db_xrefs": [ "SO:0000254" ], "anti_codon_pos": [ 43217, 43219 ], "id": "NKOCPLAKPK_2", "locus": "GCA003260355_00080" }, { "type": "tRNA", "sequence": "contig_1", "start": 43553, "stop": 43618, "strand": "+", "gene": "trnT", "product": "tRNA-Thr(tgt)", "amino_acid": "Thr", "anti_codon": "tgt", "score": 26.0, "nt": "CGCTTAGTAGCTAGAGCGATCCGATTGTCATCGGATAGGTCAGGGGTTCGACTCCCCTAGAGTCCA", "db_xrefs": [ "SO:0000270" ], "anti_codon_pos": [ 43578, 43580 ], "id": "NKOCPLAKPK_3", "locus": "GCA003260355_00081" }, { "type": "cds", "sequence": "contig_1", "start": 44019, "stop": 44393, "strand": "+", "frame": 3, "gene": null, "product": "DNA-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A929X6F0", "UniRef:UniRef90_A0A328EKT7" ], "nt": "ATGATGAAGTTTAAGTTGATTGAAAAGGTAAACCCTCTAAAAAAAGAAGACCCAAAGAAGTGGTATGCAAGTCCTATCTATGCGGATAAGGTAACACAGAAAGAGCTGGCAAAGCAACTTGCGGACCTCTCTTCACTTTCAGCTGGAGATGTTGGGAATGTAATTCAGAATCTTGTAGAGGAATTTTCCAAGATTCTGATGAGGGGAGGTATTCTCCAGTTAGGAGATTTTGGGACTTTTCGTGTAACTATCTCGAGTAAGGGAGCAGAAGAAAAAGGTAAATTTAGTGCTGAAACGATTGAGCCAAAGATTAGTTTCTTGCCAGGAGTAGAGCTCAAAAAGCAGCTTGAATCTATGAAATTTCAACAGCAATAG", "aa": "MMKFKLIEKVNPLKKEDPKKWYASPIYADKVTQKELAKQLADLSSLSAGDVGNVIQNLVEEFSKILMRGGILQLGDFGTFRVTISSKGAEEKGKFSAETIEPKISFLPGVELKKQLESMKFQQQ", "aa_hexdigest": "7cd1c0d71fdbd437b897d15e8a4a011b", "start_type": "ATG", "rbs_motif": "TTCTA", "psc": { "uniref90_id": "UniRef90_A0A328EKT7", "query_cov": 0.9919354838709677, "subject_cov": 1.0, "identity": 1.0, "score": 233.0, "evalue": 1.68e-77, "valid": true, "product": "DNA-binding protein", "uniref50_id": "UniRef50_A0A929X6F0" }, "pscc": { "uniref50_id": "UniRef50_A0A929X6F0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A929X6F0" ], "product": "Viral histone-like protein" }, "genes": [], "id": "NKOCPLAKPK_128", "locus": "GCA003260355_00082" }, { "type": "cds", "sequence": "contig_1", "start": 44579, "stop": 44791, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EJ27", "UniRef:UniRef90_A0A328EJ27" ], "nt": "ATGCCAAGAATTAAATATGAAGAAGAATGTAAATTTAGAGGCATTCTTTTCAAGAAGGGGGAAATTTTTTATGCGGATAGGATTCAATTTAAGTGGGGTTGGCGAGAAACTCGTTGGCTAGTATGGTTACTTGCTTTTGTTATTGATATTCTCCTCTTAGGTAGTGGAAGATTTCCTATCTGTGTCCTAACAAAGTTTTTATTTCCTTCGTGA", "aa": "MPRIKYEEECKFRGILFKKGEIFYADRIQFKWGWRETRWLVWLLAFVIDILLLGSGRFPICVLTKFLFPS", "aa_hexdigest": "f6abaa45873ab7b76516692e1fa5bbf5", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 44579, "stop": 45076, "observations": { "mutation": [ 44789, 44840 ], "stop": [ 44789, 44840 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EJ27", "query_cov": 1.0, "subject_cov": 0.42424242424242425, "identity": 1.0, "score": 152.0, "evalue": 4.84e-46, "gene_start": 1, "gene_end": 70, "reference_start": 1, "reference_end": 70, "reference_sequence": "MPRIKYEEECKFRGILFKKGEIFYADRIQFKWGWRETRWLVWLLAFVIDILLLGSGRFPICVLTKFLFPSGKEAPWKCRFFINEECLGWRYCPRKSFSSELSTALLKKGKIVEVQLFRQKGQVKVYKKGVFSEMSCVGNARDIGKIDDLAVLLKKYGYPFTFMEE" }, "paralog": false, "identity": 0.9878787878787879, "score": 340.0, "evalue": 2.24e-122, "description": "Internal stop codon at 44789, 44840. Nonsense mutation around 44789, 44840.", "psc": { "uniref90_id": "UniRef90_A0A328EJ27", "uniref50_id": "UniRef50_A0A328EJ27", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EJ27", "UniRef:UniRef50_A0A328EJ27" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EJ27", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EJ27" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 8517.130399999995, "isoelectric_point": 9.622309303283693 }, "id": "NKOCPLAKPK_129", "locus": "GCA003260355_00083" }, { "type": "cds", "sequence": "contig_1", "start": 44978, "stop": 45076, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCCTGCGTTGGTAATGCTAGAGATATAGGGAAGATAGATGATCTTGCGGTCCTCTTGAAAAAATATGGATATCCTTTTACTTTTATGGAAGAATAA", "aa": "MSCVGNARDIGKIDDLAVLLKKYGYPFTFMEE", "aa_hexdigest": "3ee283fc00a05910a20e99e81b509e6e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3625.195700000001, "isoelectric_point": 5.048291587829589 }, "id": "NKOCPLAKPK_130", "locus": "GCA003260355_00084" }, { "type": "cds", "sequence": "contig_1", "start": 45196, "stop": 45363, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00929.31" ], "nt": "ATGAAACTTTTCTTTTTTGATACTGAGACTACAGGAGTAAACCCAAATAAGGATAGGATTATTCAATTTGGAGCAATTTATGGCGAGTATGATCTACAAACGGGACAGTTTAATGAGTTAGAAAGAATTAATCAGTATATTAATATTGAAGGAGAGATACCCTATTGA", "aa": "MKLFFFDTETTGVNPNKDRIIQFGAIYGEYDLQTGQFNELERINQYINIEGEIPY", "aa_hexdigest": "ba726dc363003e16f54dce85d61c07d9", "start_type": "ATG", "rbs_motif": "TTGTA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00929.31", "name": "RNase_T", "length": 47, "aa_cov": 0.8363636363636363, "hmm_cov": 0.9148936170212766, "evalue": 1.8325584083091912e-08, "score": 31.52605438232422, "start": 5, "stop": 50 } ], "seq_stats": { "molecular_weight": 6495.152499999998, "isoelectric_point": 4.2507837295532225 }, "id": "NKOCPLAKPK_131", "locus": "GCA003260355_00085" }, { "type": "cds", "sequence": "contig_1", "start": 45373, "stop": 45762, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00929.31" ], "nt": "GTGCATGGTATTTACAAAAAAGATATAGAAGGTTATGGCTATATGAAAGATTATATTGATGAATTTTTGGTTTATATGGGGCGTGCGGACTATGTAATAGGTCATAATGTAGATTTTGATAAAAGGATGTTTATTTCAGAGGTAAAAAGACTTGGCAATAATTTTAATTTTGATGCAGTTCGTTGGGTAGATACGATGAGGTCGACTGCTCAATTTGTAAATGAGAAAGGAGGAAAGCGACCTAAACTCATTAAGTTGCATGAAAAGCTCTTTGGTAGGGGGTTTGATGGGGCTCATGATGCAATGGCTGATATCGTAGCTACGAAAGATTGTTTTTTGGAATTAAGGAAATATGGCTTTTTTCGTGAAATTTTTAGTTTACCTTTATGA", "aa": "MHGIYKKDIEGYGYMKDYIDEFLVYMGRADYVIGHNVDFDKRMFISEVKRLGNNFNFDAVRWVDTMRSTAQFVNEKGGKRPKLIKLHEKLFGRGFDGAHDAMADIVATKDCFLELRKYGFFREIFSLPL", "aa_hexdigest": "c276265c9fd4afa1506dbeb645afc0f4", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00929.31", "name": "RNase_T", "length": 110, "aa_cov": 0.8449612403100775, "hmm_cov": 0.9636363636363636, "evalue": 8.393112119170579e-08, "score": 29.376853942871094, "start": 1, "stop": 109 } ], "seq_stats": { "molecular_weight": 15136.289299999986, "isoelectric_point": 8.608737373352053 }, "id": "NKOCPLAKPK_132", "locus": "GCA003260355_00086" }, { "type": "cds", "sequence": "contig_1", "start": 45944, "stop": 46318, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATACAATTGGTGATTTTCGTGATTGATATGCTCAAGCTCGTATATGAGACAAGCGGGGGATTATCAATGGAAAATGAGAAGAAGTCTGTAAAATCCAATATGATGCAATTGGTGATTTCCGTTGTTGATATGCTCAAGTTCGTATTTGAGATAAGCGAGGGGTTATTAATAAGCAGTGGAAAGAGATTTGTGAAATCAAATATGATGCAATTGGTAATTTTCGTGTTTGATGTGTTAAAGTTTGTATATGAGACAAACGAGGAATTGTCAATGGAAAATGAGACGAGGTCTGTAAAATTAAATATGATGCAATTGGTGATTTTTATGATTGATATGCTCAAGTTTGTGTTTGAGATAAACGAGGGATTATAA", "aa": "MIQLVIFVIDMLKLVYETSGGLSMENEKKSVKSNMMQLVISVVDMLKFVFEISEGLLISSGKRFVKSNMMQLVIFVFDVLKFVYETNEELSMENETRSVKLNMMQLVIFMIDMLKFVFEINEGL", "aa_hexdigest": "7d50f690666a055436f5b7c13ced3e72", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14398.149199999993, "isoelectric_point": 4.8507192611694325 }, "id": "NKOCPLAKPK_133", "locus": "GCA003260355_00087" }, { "type": "cds", "sequence": "contig_1", "start": 46319, "stop": 46420, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGCGATGAAAAGAGGGGTGTGAAATTAAATATGATGAAGTTGGTGATTTTTGTGATTGATATGCTCAAGTTCGTGTTTGAGATAAACGAGGAATTATAA", "aa": "MGDEKRGVKLNMMKLVIFVIDMLKFVFEINEEL", "aa_hexdigest": "69ffbdf500dcbaf9afc7005c523e2b5b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3930.7613999999994, "isoelectric_point": 5.052270317077636 }, "id": "NKOCPLAKPK_134", "locus": "GCA003260355_00088" }, { "type": "cds", "sequence": "contig_1", "start": 46625, "stop": 46741, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGGGAGGAGAACAGCTCTGTGAAATTAAATATGATGCAATTGGTGATTTTTGTGATTGATATGCTCAAGTTCGTATTTGAGAGAAACGAGGAATTATTAATGAAAAATGAGTAG", "aa": "MGEENSSVKLNMMQLVIFVIDMLKFVFERNEELLMKNE", "aa_hexdigest": "a4b7c6b3d1dfea465bfca2a5d3261e26", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4550.363399999998, "isoelectric_point": 4.667754554748535 }, "id": "NKOCPLAKPK_135", "locus": "GCA003260355_00089" }, { "type": "cds", "sequence": "contig_1", "start": 47470, "stop": 47673, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13306.12" ], "nt": "ATGGAGGCTGATGCATTTGCAGATAATAGGCTGATTTCGGTTTATTTACCTAAAAATTTGAAAACGATCCGTTCTTGTGCTTTTGCAAAAAACCGTATTCAGTCGCTTATTTTACCAGAAGGACTTCAGTATCTTGGTTATGCTGCATTTGCAGACAATCAGATTGCCTCCATCCATTTCCCTAATTCACTTAAGAGGATGTGA", "aa": "MEADAFADNRLISVYLPKNLKTIRSCAFAKNRIQSLILPEGLQYLGYAAFADNQIASIHFPNSLKRM", "aa_hexdigest": "356462aa927538b3420aa994b2ce7f32", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13306.12", "name": "LRR_5", "length": 65, "aa_cov": 0.9552238805970149, "hmm_cov": 1.0, "evalue": 1.7629711518125094e-13, "score": 46.999794006347656, "start": 3, "stop": 66 } ], "seq_stats": { "molecular_weight": 7559.704199999998, "isoelectric_point": 9.358246803283691 }, "id": "NKOCPLAKPK_136", "locus": "GCA003260355_00090" }, { "type": "cds", "sequence": "contig_1", "start": 47713, "stop": 47877, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13306.12" ], "nt": "TTGCCTGAAGGTTTGGAAAAATTAGAATATGCTGTTTTTTCACATAATAAATTGATAGGTGTAAAGTTCCCACAAGACCTTAGGAGTATTGGAGCCTATGCTTTCCATGATAATCTCCTTACGGTGGTTAATATCCCTCCAGGGGTAGAGGAAATAGGGACATGA", "aa": "MPEGLEKLEYAVFSHNKLIGVKFPQDLRSIGAYAFHDNLLTVVNIPPGVEEIGT", "aa_hexdigest": "676950dea1b716017c8e032a3b6681da", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13306.12", "name": "LRR_5", "length": 53, "aa_cov": 0.9629629629629629, "hmm_cov": 1.0, "evalue": 1.1207481436103742e-08, "score": 31.47426414489746, "start": 2, "stop": 53 } ], "seq_stats": { "molecular_weight": 5968.7865, "isoelectric_point": 5.048121070861816 }, "id": "NKOCPLAKPK_137", "locus": "GCA003260355_00091" }, { "type": "cds", "sequence": "contig_1", "start": 47884, "stop": 48009, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGGAATGAATTAGTAAGATTGGATCTTCCAGATGAAGTAGAAGTTGTGCAGCCTTATTCTTTTGCTAAAAATAAGATAATTAGCCTCAATTTCCCTACTTCTGTGGAAGAGATTAAAAAATGA", "aa": "MGNELVRLDLPDEVEVVQPYSFAKNKIISLNFPTSVEEIKK", "aa_hexdigest": "dd2166a8afaa27b15cb337ccb9b4b8e1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4691.3576, "isoelectric_point": 4.7668249130249025 }, "id": "NKOCPLAKPK_138", "locus": "GCA003260355_00092" }, { "type": "cds", "sequence": "contig_1", "start": 48656, "stop": 48799, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAATAATCAATATTCTTGCAAATCTTTATGATACCTTTTTGTGCTATCATACAGAGAAACTCATTCGTCATACAAATTCCTATAAGCAGAAAAATTTAAGTACTTTCAATGTATTTTGTCTTGTAAGTGGTTTATAA", "aa": "MKKIINILANLYDTFLCYHTEKLIRHTNSYKQKNLSTFNVFCLVSGL", "aa_hexdigest": "5197eee4c62d2dcb7b37a222f66a014e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5537.4798, "isoelectric_point": 9.3911901473999 }, "id": "NKOCPLAKPK_139", "locus": "GCA003260355_00093" }, { "type": "cds", "sequence": "contig_1", "start": 48802, "stop": 48906, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGAGGATGTAAAATTTAGGGATGATCATAATGATGCTTTTGTAAATCAGGAAAAAAAACAATATGAAGGTTATTTTAAACAACTAGATGACAAAGGTTTTTGA", "aa": "MEDVKFRDDHNDAFVNQEKKQYEGYFKQLDDKGF", "aa_hexdigest": "634acc07febefc64f659d9f212ed63b0", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4185.498700000002, "isoelectric_point": 4.749375343322753 }, "id": "NKOCPLAKPK_140", "locus": "GCA003260355_00094" }, { "type": "cds", "sequence": "contig_1", "start": 49294, "stop": 49476, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAGTTATGGAATTGGTTATGGATAGGTTGAAAAAGGATGAATTTTTACAACCTATATTATCTAAATATTTACTCTATTTTAGTAGTCCTGAATATTCAGTTGAAGAGTTTGAAACGCTAGATGACTATTATAACAGAGTTAAGAGGTCTTATCTAACTTTGATTAAGGATTTTAAGTGA", "aa": "MEVMELVMDRLKKDEFLQPILSKYLLYFSSPEYSVEEFETLDDYYNRVKRSYLTLIKDFK", "aa_hexdigest": "5b268cd9ca09553e8ad5759d27199b2d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7411.4776999999995, "isoelectric_point": 4.792743492126465 }, "id": "NKOCPLAKPK_141", "locus": "GCA003260355_00095" }, { "type": "cds", "sequence": "contig_1", "start": 49559, "stop": 49861, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATTGTGGTTGCAACTGAACACCATATTTTTTCAATTCTTTCTCTAATTTATTTAGAAAATACCACTTTCAGTCCTGATAATCAGCATATCTCATATCAATCAATTTATTTCAAGATTTTTTATGATTTTCTTTTTTTTGATTCATCCTATGTACATAATTCTCAGCCGATATATAAGGAGCCGTATTTCAATTTTTATCTATTCAAAAATATTCAATATAAATACCATAATCTGGCAAATAAAAATCTGGTTTATAAGTTCTTCTATCTCAAGTATCTGTTGTGTAAAAGTGTGATTTAG", "aa": "MIVVATEHHIFSILSLIYLENTTFSPDNQHISYQSIYFKIFYDFLFFDSSYVHNSQPIYKEPYFNFYLFKNIQYKYHNLANKNLVYKFFYLKYLLCKSVI", "aa_hexdigest": "bf3c0d1ce908f46e875b674194f6ce75", "start_type": "GTG", "rbs_motif": "TTTA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12288.023899999987, "isoelectric_point": 8.577276802062986 }, "id": "NKOCPLAKPK_142", "locus": "GCA003260355_00096" }, { "type": "cds", "sequence": "contig_1", "start": 49903, "stop": 50076, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGATAATATCTTAAAAACTTTTTTAGCTCTTTATAAAGAATCGAGAGAAACGATTGAGGCGCTAAAAGAAAAGGTGCAAACATTTGATGGATTAAATAGAGAAAGAAATCAGATTTTTTTAGAAATATTTTCTCACTATTTTAAGCAGTATACAGATCTTTTAGTGGAATGA", "aa": "MDNILKTFLALYKESRETIEALKEKVQTFDGLNRERNQIFLEIFSHYFKQYTDLLVE", "aa_hexdigest": "571bd2d113c685831d8c45c61b719220", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6930.843899999998, "isoelectric_point": 5.318902015686036 }, "id": "NKOCPLAKPK_143", "locus": "GCA003260355_00097" }, { "type": "cds", "sequence": "contig_1", "start": 50098, "stop": 50256, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00580.27" ], "nt": "ATGATATCAGATGCTAAAGATATCATAAAATCAGGAGCCGTTCAAAAAAAATATTCATATATACTAGTGGATGAATTTCAGGATATATCAGAAGCTAGGGCGATGCTTCTAAGAAATCTGATTAAAGATCATGACGAGACAAGATTATTTTGTGTATGA", "aa": "MISDAKDIIKSGAVQKKYSYILVDEFQDISEARAMLLRNLIKDHDETRLFCV", "aa_hexdigest": "4f01de55b7590334539903935d92222f", "start_type": "ATG", "rbs_motif": "TTTA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00580.27", "name": "UvrD-helicase", "length": 40, "aa_cov": 0.7692307692307693, "hmm_cov": 0.95, "evalue": 5.6259841403748165e-09, "score": 32.48075866699219, "start": 13, "stop": 52 } ], "seq_stats": { "molecular_weight": 6031.932099999998, "isoelectric_point": 5.628162956237793 }, "id": "NKOCPLAKPK_144", "locus": "GCA003260355_00098" }, { "type": "tRNA", "sequence": "contig_1", "start": 52075, "stop": 52157, "strand": "+", "gene": "trnY", "product": "tRNA-Tyr(gta)", "amino_acid": "Tyr", "anti_codon": "gta", "score": 68.0, "nt": "GGGGAGATGGCAGAGCGGTCAATTGCATCAGACTGTAAATCTGACGCCTCACGGCTACGTAGGTTCAAATCCTACTCTCCCCA", "db_xrefs": [ "SO:0000272" ], "anti_codon_pos": [ 52109, 52111 ], "id": "NKOCPLAKPK_4", "locus": "GCA003260355_00099" }, { "type": "tRNA", "sequence": "contig_1", "start": 52176, "stop": 52247, "strand": "+", "gene": "trnR", "product": "tRNA-Arg(tct)", "amino_acid": "Arg", "anti_codon": "tct", "score": 58.8, "nt": "GTCCCTATAGTTCAATGGATAGAACAACAGCCTTCTAAGCTGTGGATCCAGGTTCGATCCCTGGTGGGGATA", "db_xrefs": [ "SO:0001036" ], "anti_codon_pos": [ 52209, 52211 ], "id": "NKOCPLAKPK_5", "locus": "GCA003260355_00100" }, { "type": "tRNA", "sequence": "contig_1", "start": 52267, "stop": 52340, "strand": "+", "gene": "trnE", "product": "tRNA-Glu(ctc)", "amino_acid": "Glu", "anti_codon": "ctc", "score": 67.4, "nt": "GCTCTCGTGGTCTAGTGGTTTAGGACGCCACCCTCTCACGGTGGAAATCGAGGGTTCGAGTCCCTCCGGGAGCG", "db_xrefs": [ "SO:0000259" ], "anti_codon_pos": [ 52268, 52270 ], "id": "NKOCPLAKPK_6", "locus": "GCA003260355_00101" }, { "type": "cds", "sequence": "contig_1", "start": 52581, "stop": 52988, "strand": "+", "frame": 3, "gene": "infC", "product": "translation initiation factor IF-3", "db_xrefs": [ "GO:0003743", "GO:0006413", "SO:0001217", "UniRef:UniRef50_A0A288DGP1", "UniRef:UniRef90_A0A660MSN9" ], "nt": "ATGGCTCAGGAAAAGGGGCTTGATTTGGTTCAATTGACTTATGATCCTGAGAAGATGCTCTCTACGGTGAGGATGACGGATTATGGAAAGTATATGTATCAAAAAGGAAAAGAAGAAAAAGAAAGAAGGAAGATTCAGAAGGGAAAAGATACAAAAGAATTGAAAGTGAGTTATTCTATAGGAGATAACGATCTTCAGCTCAAGATAAAGAAAGCACAGGAAATTCTTCAGGACGGAGATAATGTGAAATTTTCTATCAGGCTGAAGGGGCGTGAGAGAATGTATGCTGATAAAGCGGTTCAAAAACTGAAGCTTATTCAGGATTCGCTTGTGGAGTATGGAAAATCTCAGTATGATACTCCCAAAAAAGAAGTAAACGGATATAGTGTTATTTTATTTAGTAAATAG", "aa": "MAQEKGLDLVQLTYDPEKMLSTVRMTDYGKYMYQKGKEEKERRKIQKGKDTKELKVSYSIGDNDLQLKIKKAQEILQDGDNVKFSIRLKGRERMYADKAVQKLKLIQDSLVEYGKSQYDTPKKEVNGYSVILFSK", "aa_hexdigest": "303b84fab0c6f92b1b32d4333c8f511f", "start_type": "ATG", "rbs_motif": null, "genes": [ "infC" ], "truncated": "5-prime", "pseudogene": { "start": 52368, "stop": 52988, "observations": { "mutation": [ 52413, 52416, 52434, 52551 ], "stop": [ 52413, 52416, 52434, 52551 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A660MSN9", "query_cov": 1.0, "subject_cov": 0.6553398058252428, "identity": 0.985, "score": 253.0, "evalue": 7.17e-84, "gene_start": 1, "gene_end": 135, "reference_start": 72, "reference_end": 206, "reference_sequence": "MDQKTFKASGTNQNFGGPKKAFGGQKSDKGGMNAFKAKMIYVNDQIKAPSVMIIDDEKNNLGTFPRRIALEMAQEKGLDLVQLTYDPEKMLSTVRMTDYGKYMYQKGKEEKERRKTQKGKDTKELKVSYSIGDNDLQLKIKKAQEILQDGDNVKFSIRLKGRERMYADKAVQKLKLIQDSLVEYGKSQYDTPKKEANGYSVILFSK" }, "paralog": false, "identity": 0.9271844660194175, "score": 368.0, "evalue": 1.22e-131, "description": "Internal stop codon at 52413, 52416, 52434, 52551. Nonsense mutation around 52413, 52416, 52434, 52551.", "psc": { "uniref90_id": "UniRef90_A0A660MSN9", "gene": "infC", "product": "translation initiation factor IF-3", "uniref50_id": "UniRef50_A0A288DGP1", "go_ids": [ "GO:0003743", "GO:0006413" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A660MSN9", "UniRef:UniRef50_A0A288DGP1", "GO:0003743", "GO:0006413" ] }, "pscc": { "uniref50_id": "UniRef50_A0A288DGP1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A288DGP1" ], "product": "Translation initiation factor IF-3" } }, "id": "NKOCPLAKPK_145", "locus": "GCA003260355_00102" }, { "type": "cds", "sequence": "contig_1", "start": 52998, "stop": 53132, "strand": "+", "frame": 3, "gene": null, "product": "50S ribosomal protein L35", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7W4EBQ7", "UniRef:UniRef90_A0A660MS40" ], "nt": "ATGGCTAAGAAACTTAAATCGCATTCTGGAGCACTTAAAAGGTTTAAGATTACCAAGAAAGATAAAGTGTTGTCTAGTAAGCAGTGTAATAATCATCTTTTGACTAATAAAGGAAAGAATAATAAAAAATTCTGA", "aa": "MAKKLKSHSGALKRFKITKKDKVLSSKQCNNHLLTNKGKNNKKF", "aa_hexdigest": "6424a8a8b2006facd34d486454fee108", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 52998, "stop": 53195, "observations": { "mutation": [ 53130 ], "stop": [ 53130 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A660MS40", "query_cov": 1.0, "subject_cov": 0.676923076923077, "identity": 1.0, "score": 87.0, "evalue": 9.71e-22, "gene_start": 1, "gene_end": 44, "reference_start": 1, "reference_end": 44, "reference_sequence": "MAKKLKSHSGALKRFKITKKDKVLSSKQCNNHLLTNKGKNNKKFGYGKEVVGAAAKMLKNLLPYK" }, "paralog": false, "identity": 0.9846153846153847, "score": 129.0, "evalue": 5.42e-41, "description": "Internal stop codon at 53130. Nonsense mutation around 53130.", "psc": { "uniref90_id": "UniRef90_A0A660MS40", "product": "50S ribosomal protein L35", "uniref50_id": "UniRef50_A0A7W4EBQ7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A660MS40", "UniRef:UniRef50_A0A7W4EBQ7" ] }, "pscc": { "uniref50_id": "UniRef50_A0A7W4EBQ7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7W4EBQ7" ], "product": "50S ribosomal protein L35" } }, "id": "NKOCPLAKPK_146", "locus": "GCA003260355_00103" }, { "type": "cds", "sequence": "contig_1", "start": 53204, "stop": 53551, "strand": "+", "frame": 2, "gene": "rplT", "product": "50S ribosomal protein L20", "db_xrefs": [ "GO:0000315", "GO:0003735", "GO:0006412", "GO:0022625", "SO:0001217", "UniParc:UPI000DBFA2F0", "UniRef:UniRef100_A0A328EJ32", "UniRef:UniRef50_A0A288DDH7", "UniRef:UniRef90_A0A2W4IL16" ], "nt": "ATGGTAAGAGTAACGAATGGATTACAGAGGCAGAGAAGGCATAAGAAATTTATTGCTCAGGCTTCTGGGTTTAGATTGGGAAGAAAAAATCTCTACAAGCAGGTAAGGCTTGCTTTGGTAAAACAGGGTCAGCATGCTTATGTAAGTAGAAGGCTTAAGAAAAGGGATTTTAGAAGACTCTGGATTGAGAGGCTTTCTGCTGCGGTTAGAGAAAAAGGTGGAAAATATTCAGTATTTGTAAATACTATGTATCAAAAGGATATTAAACTTGATAGAAAGGTGCTTTCAAATATTGCGGTAGCATTTCCTGCCGTGTTTGATAAAATTTATGATGAGGTGGTAAAATAA", "aa": "MVRVTNGLQRQRRHKKFIAQASGFRLGRKNLYKQVRLALVKQGQHAYVSRRLKKRDFRRLWIERLSAAVREKGGKYSVFVNTMYQKDIKLDRKVLSNIAVAFPAVFDKIYDEVVK", "aa_hexdigest": "3bcd47b49480b7350748eaa3288523fa", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000DBFA2F0", "uniref100_id": "UniRef100_A0A328EJ32", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBFA2F0", "UniRef:UniRef100_A0A328EJ32" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EJ32", "uniref90_id": "UniRef90_A0A2W4IL16" }, "psc": { "uniref90_id": "UniRef90_A0A2W4IL16", "gene": "rplT", "product": "50S ribosomal protein L20", "uniref50_id": "UniRef50_A0A288DDH7", "go_ids": [ "GO:0000315", "GO:0003735", "GO:0006412", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_A0A288DDH7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A288DDH7" ], "product": "Large ribosomal subunit protein bL20" }, "genes": [ "rplT" ], "id": "NKOCPLAKPK_147", "locus": "GCA003260355_00104" }, { "type": "tRNA", "sequence": "contig_1", "start": 53603, "stop": 53689, "strand": "+", "gene": "trnL", "product": "tRNA-Leu(taa)", "amino_acid": "Leu", "anti_codon": "taa", "score": 69.8, "nt": "GCCGAGGTGGTGGAATTGGTAGACACGAAGGACTTAAAATCCTTTGAGGCTTATACCCTCGTGCCGGTTCGATTCCGGTCCTCGGCA", "db_xrefs": [ "SO:0000264" ], "anti_codon_pos": [ 53637, 53639 ], "id": "NKOCPLAKPK_7", "locus": "GCA003260355_00105" }, { "type": "cds", "sequence": "contig_1", "start": 53772, "stop": 54005, "strand": "+", "frame": 3, "gene": null, "product": "Transposase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4IJV2", "UniRef:UniRef90_A0A2W4IJV2" ], "nt": "TTGAATACCAACGAAAGACTTATTTCTTTCTACAAGAAAGAGCTTTGGGCTTCTCATATTTTGGATAAGGCAAAGAAACAACAACACTATGGTACTAAATGGTATAGAAAGTTTGTAAGTTATGATTTTCCTTCTTCTAGAAGATATAAGAGAATGAGAGCTATCGTATCTGATGCTTATAGAAAGACAAAGAAGAAGTATAATACTTTGGCACAACTTGGAAAGATTGTGTAA", "aa": "MNTNERLISFYKKELWASHILDKAKKQQHYGTKWYRKFVSYDFPSSRRYKRMRAIVSDAYRKTKKKYNTLAQLGKIV", "aa_hexdigest": "85bc31e329507df4695f0abc2621f0a6", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A2W4IJV2", "query_cov": 1.0, "subject_cov": 0.8850574712643678, "identity": 0.922, "score": 145.0, "evalue": 3.28e-44, "valid": true, "product": "Transposase", "uniref50_id": "UniRef50_A0A2W4IJV2" }, "pscc": { "uniref50_id": "UniRef50_A0A2W4IJV2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4IJV2" ], "product": "Transposase" }, "genes": [], "id": "NKOCPLAKPK_148", "locus": "GCA003260355_00106" }, { "type": "cds", "sequence": "contig_1", "start": 54032, "stop": 54349, "strand": "+", "frame": 2, "gene": "rplU", "product": "50S ribosomal protein L21", "db_xrefs": [ "GO:0003723", "GO:0003735", "GO:0005840", "GO:0006412", "KEGG:K02888", "SO:0001217", "UniParc:UPI000DBF87BA", "UniRef:UniRef100_A0A328EJM0", "UniRef:UniRef50_A0A288DA01", "UniRef:UniRef90_A0A328EJM0" ], "nt": "ATGTATGCAGTGGTAGATTTGCAAGGGCATCAATATATTGTTTCAGAAGGAGCTGAACTTATTGTAGATAATGTAGATAAGGCACAAGGTGAAAAGTTGCTTTCTGATAAAGTGCTTGCACTCTTTGATGAGAAGGGTACTGAAGTAAAGGTAGGAACGCCTTATATTTCTGGGGCTCAGGTTGAATTTGAGGTTGGAACTACTCAAAAGGGAGAAAAGGTTAGAATCTTAAAGTTTAAGAGAAAGACAAGATATGCGAGATTGAAAGGATTTAGACCTCTTCAAACGGTTTTAACTGTTAAAAACATTAGTGCTTAA", "aa": "MYAVVDLQGHQYIVSEGAELIVDNVDKAQGEKLLSDKVLALFDEKGTEVKVGTPYISGAQVEFEVGTTQKGEKVRILKFKRKTRYARLKGFRPLQTVLTVKNISA", "aa_hexdigest": "0bd0311d7fc99b2b9cfeee00935aae6e", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000DBF87BA", "uniref100_id": "UniRef100_A0A328EJM0", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBF87BA", "UniRef:UniRef100_A0A328EJM0" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EJM0", "uniref90_id": "UniRef90_A0A328EJM0" }, "psc": { "uniref90_id": "UniRef90_A0A328EJM0", "gene": "rplU", "product": "50S ribosomal protein L21", "uniref50_id": "UniRef50_A0A288DA01", "kegg_orthology_id": "K02888", "go_ids": [ "GO:0003723", "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_A0A288DA01", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A288DA01" ], "product": "Large ribosomal subunit protein bL21" }, "genes": [ "rplU" ], "id": "NKOCPLAKPK_149", "locus": "GCA003260355_00107" }, { "type": "cds", "sequence": "contig_1", "start": 54367, "stop": 54546, "strand": "+", "frame": 1, "gene": "infA", "product": "translation initiation factor IF-1", "db_xrefs": [ "GO:0003743", "GO:0006413", "KEGG:K02518", "SO:0001217", "UniRef:UniRef50_A0A288DHU9", "UniRef:UniRef90_A0A660MQ36" ], "nt": "TTGGAACGAGATGGCGAAATATTGGAACAACTTCCTGCTGGGAAGTATATGGTTAAGCTTAAGGATACTGATATGGTAATTTCTTGCTATAAGGCAGGGAAGATGAAACAATCGCATATTGGAATTATTGAGGGAGATCGAGTGAAGGTTGAGGTTAATCAATATGATATGAAACAATGA", "aa": "MERDGEILEQLPAGKYMVKLKDTDMVISCYKAGKMKQSHIGIIEGDRVKVEVNQYDMKQ", "aa_hexdigest": "805d8702221a5a1dc50039d83a515866", "start_type": "TTG", "rbs_motif": null, "genes": [ "infA" ], "truncated": "both", "pseudogene": { "start": 54349, "stop": 54603, "observations": { "mutation": [ 54361, 54544 ], "stop": [ 54361, 54544 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A660MQ36", "query_cov": 1.0, "subject_cov": 0.7023809523809523, "identity": 0.983, "score": 118.0, "evalue": 1.36e-33, "gene_start": 1, "gene_end": 59, "reference_start": 7, "reference_end": 65, "reference_sequence": "MAKAGVLERDGEILEQLPAGKYMVKLKDTDMVISCYKAGKMKQSHIGIIEGDRVKVEVNQYDMKQGRIVYRYNVATKAQAHENE" }, "paralog": false, "identity": 0.9761904761904762, "score": 162.0, "evalue": 1.57e-53, "description": "Internal stop codon at 54361, 54544. Nonsense mutation around 54361, 54544.", "psc": { "uniref90_id": "UniRef90_A0A660MQ36", "gene": "infA", "product": "translation initiation factor IF-1", "uniref50_id": "UniRef50_A0A288DHU9", "kegg_orthology_id": "K02518", "go_ids": [ "GO:0003743", "GO:0006413" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A660MQ36", "UniRef:UniRef50_A0A288DHU9", "KEGG:K02518", "GO:0003743", "GO:0006413" ] }, "pscc": { "uniref50_id": "UniRef50_A0A288DHU9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A288DHU9" ], "product": "Translation initiation factor IF-1" } }, "id": "NKOCPLAKPK_150", "locus": "GCA003260355_00108" }, { "type": "cds", "sequence": "contig_1", "start": 54780, "stop": 55076, "strand": "+", "frame": 3, "gene": "rpsM", "product": "30S ribosomal protein S13", "db_xrefs": [ "GO:0003723", "GO:0003735", "GO:0005840", "GO:0006412", "KEGG:K02952", "SO:0001217", "UniRef:UniRef50_A0A288GT71", "UniRef:UniRef90_A0A7W4HMV3" ], "nt": "ATGTTTAGATTGATGGGACATGTTCTTCCAGAAGAAAAATCTATTTGGATTGCGTTGACGATGATTTATGGAATTGGAAGAGAAAGATCAAAGAAAATCTTGGGGAACCTTAACATTCCATATATGAAGAAAGTAAGTGAAATCTCAGAAGAAGAGCAAAAGTTAATCTCTGATGAATTGAAGAAGTATGTTTTGGAGAATGATCTCAAGAGGGAGGTTGCTTCTGCAATCAAAAGACTTAAGGAAATTAAGTGTTATAGAGGAATGAGGCATAACCTTGGACTTCCTGTAAGATGA", "aa": "MFRLMGHVLPEEKSIWIALTMIYGIGRERSKKILGNLNIPYMKKVSEISEEEQKLISDELKKYVLENDLKREVASAIKRLKEIKCYRGMRHNLGLPVR", "aa_hexdigest": "e9b3b68e605aee1c21af953cc90e2c1d", "start_type": "ATG", "rbs_motif": "TTATT", "psc": { "uniref90_id": "UniRef90_A0A1V5ZLU9", "query_cov": 0.8163265306122449, "subject_cov": 1.0, "identity": 0.8, "score": 124.0, "evalue": 1.55e-35, "valid": false, "product": "30S ribosomal protein S13", "uniref50_id": "UniRef50_A0A9D8B9E7", "kegg_orthology_id": "K02952" }, "genes": [ "rpsM" ], "truncated": "3-prime", "pseudogene": { "start": 54780, "stop": 55154, "observations": { "mutation": [ 55074, 55095 ], "stop": [ 55074, 55095 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A7W4HMV3", "query_cov": 1.0, "subject_cov": 0.7903225806451613, "identity": 0.9079999999999999, "score": 168.0, "evalue": 3.51e-52, "gene_start": 1, "gene_end": 98, "reference_start": 1, "reference_end": 98, "reference_sequence": "MFRLMGHVLPEEKSIWIALTNIYGIGRERSKKILTALNIEIMKKVRDISEEEQKMISDELKKYVLENDLKREVAAAIKRLKEIKCYRGMRHNLGLPVRGQLTRKNARTAKKLLGRSKVRPVLKK" }, "paralog": false, "identity": 0.9112903225806451, "score": 217.0, "evalue": 4.55e-74, "description": "Internal stop codon at 55074, 55095. Nonsense mutation around 55074, 55095.", "psc": { "uniref90_id": "UniRef90_A0A7W4HMV3", "gene": "rpsM", "product": "30S ribosomal protein S13", "uniref50_id": "UniRef50_A0A288GT71", "kegg_orthology_id": "K02952", "go_ids": [ "GO:0003723", "GO:0003735", "GO:0005840", "GO:0006412" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A7W4HMV3", "UniRef:UniRef50_A0A288GT71", "KEGG:K02952", "GO:0003723", "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_A0A288GT71", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A288GT71" ], "product": "Small ribosomal subunit protein uS13" } }, "id": "NKOCPLAKPK_151", "locus": "GCA003260355_00109" }, { "type": "cds", "sequence": "contig_1", "start": 55180, "stop": 55419, "strand": "+", "frame": 1, "gene": null, "product": "Small ribosomal subunit protein uS11", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2M7XKB0", "UniRef:UniRef90_A0A660MRR8" ], "nt": "ATGGCTGTTACGAAGGCAAAGAAAAAAGATATTAAAGTGGTAAGTGGGGTATTGCATGTGCATACCACTCCTAATAATACTTTAGTGACTTTGATTGATCAAGAAGGAAATAAAGTTCTTGGTGGTGGAACAGGAAAGGTTGGCTACAAGGGCTCAAAAAAGAGTACTCCTTATGCGGCAGAAGTGCTTACTAAGCAAATTCTTAAAGATGCTCAGAATTATGGATTGAAGGAAATCTGA", "aa": "MAVTKAKKKDIKVVSGVLHVHTTPNNTLVTLIDQEGNKVLGGGTGKVGYKGSKKSTPYAAEVLTKQILKDAQNYGLKEI", "aa_hexdigest": "b1d2634b1b3944f25f10eaa49c0f2cea", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 55180, "stop": 55563, "observations": { "mutation": [ 55417 ], "stop": [ 55417 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A660MRR8", "query_cov": 1.0, "subject_cov": 0.6220472440944882, "identity": 0.937, "score": 146.0, "evalue": 8.52e-44, "gene_start": 1, "gene_end": 79, "reference_start": 1, "reference_end": 79, "reference_sequence": "MAVTKAKKKDIKVVSGVLHVHTTSNNTLITLIDQEGNKILGGGTGKVGYKGSKKSTPYAAEVLTKQILKDGQGYGLKEIGIVFKGIGMAREGVFKAINEVGVIDIAYIKEQTGIQFGGCKGERPKRN" }, "paralog": false, "identity": 0.905511811023622, "score": 237.0, "evalue": 3.42e-82, "description": "Internal stop codon at 55417. Nonsense mutation around 55417.", "psc": { "uniref90_id": "UniRef90_A0A660MRR8", "product": "Small ribosomal subunit protein uS11", "uniref50_id": "UniRef50_A0A2M7XKB0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A660MRR8", "UniRef:UniRef50_A0A2M7XKB0" ] }, "pscc": { "uniref50_id": "UniRef50_A0A2M7XKB0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2M7XKB0" ], "product": "Small ribosomal subunit protein uS11" } }, "id": "NKOCPLAKPK_152", "locus": "GCA003260355_00110" }, { "type": "cds", "sequence": "contig_1", "start": 55441, "stop": 55563, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAAGAGAAGGAGTTTTTAAGGCGGTTACCGAGATTGGAGTAATTGATGTTGCTTATATTAAAGAAGAAACAGGAATTCAATTCGGTGGATGTAAAGGAGAAAGACCAAAGAGAAACTAA", "aa": "MAREGVFKAVTEIGVIDVAYIKEETGIQFGGCKGERPKRN", "aa_hexdigest": "b4d06f69a1b7cc9451825d9364d6793b", "start_type": "ATG", "rbs_motif": "TTTTTA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4398.029400000001, "isoelectric_point": 7.901132392883301 }, "id": "NKOCPLAKPK_153", "locus": "GCA003260355_00111" }, { "type": "cds", "sequence": "contig_1", "start": 55624, "stop": 56244, "strand": "+", "frame": 1, "gene": "rpsD", "product": "30S ribosomal protein S4", "db_xrefs": [ "GO:0003735", "GO:0006412", "GO:0015935", "GO:0019843", "KEGG:K02986", "SO:0001217", "UniParc:UPI000DBFDADB", "UniRef:UniRef100_A0A660MRY3", "UniRef:UniRef50_A0A288DK80", "UniRef:UniRef90_A0A660MRY3" ], "nt": "ATGAGATACACAGGAGCTAAATTTAGACTATGTAGGAGGGAAGGAGTAAATCTTTTCGGTTCTCAGAAGTATAATCTCAAGAAGAGAAGGGCTTTGCCTGGACAGCATGGAGCAGGAATGCAGAGAAATTCTGAATATGGGAAACTTTTGAGGAATAAGCAAGTAGTGAAGAGAAGTTATCTTCTTTCAGAGAAACAGTTTGCAAATATTGTGAAGAAAACTGCTGCTAAATATGCTAAAAACAATAACCTTTCTCATGATGTAGCGTTGTTTCAGTTTTTGGAGAGGAGACTTGATGTTATTATCTTGAGGTCAGGACTTGCAAATACAATCATGCAGGCGAGACAAATGGTGAGTCATGGTCACTTCCTTCTTAATGGAGTAAAACATAATGTTCCTTCCACATTTGTTGTTGCAGGAGATAAGATTACTCTTAAAGATAAGCTTAAGAATTCAGCGTTGTATTCTGATGCGTCAAAGGCTTCCCCTGCTAAAATCCCTTCTTGGATTAAAGTGGATAGAAATAGTTATGCTGTAGAGGTACTTGGAATGCCACAGACTGGAGAGATTGAGATACTTGGAGATCTCTTGAAGGTGATTGAATTCTATGCGAGGGCTTAA", "aa": "MRYTGAKFRLCRREGVNLFGSQKYNLKKRRALPGQHGAGMQRNSEYGKLLRNKQVVKRSYLLSEKQFANIVKKTAAKYAKNNNLSHDVALFQFLERRLDVIILRSGLANTIMQARQMVSHGHFLLNGVKHNVPSTFVVAGDKITLKDKLKNSALYSDASKASPAKIPSWIKVDRNSYAVEVLGMPQTGEIEILGDLLKVIEFYARA", "aa_hexdigest": "49b72606c989d088140d9cfccf7fa346", "start_type": "ATG", "rbs_motif": "TTATT", "ups": { "uniparc_id": "UPI000DBFDADB", "uniref100_id": "UniRef100_A0A660MRY3", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBFDADB", "UniRef:UniRef100_A0A660MRY3" ] }, "ips": { "uniref100_id": "UniRef100_A0A660MRY3", "uniref90_id": "UniRef90_A0A660MRY3" }, "psc": { "uniref90_id": "UniRef90_A0A660MRY3", "gene": "rpsD", "product": "30S ribosomal protein S4", "uniref50_id": "UniRef50_A0A288DK80", "kegg_orthology_id": "K02986", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015935", "GO:0019843" ] }, "pscc": { "uniref50_id": "UniRef50_A0A288DK80", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A288DK80" ], "product": "Small ribosomal subunit protein uS4" }, "genes": [ "rpsD" ], "id": "NKOCPLAKPK_154", "locus": "GCA003260355_00112" }, { "type": "cds", "sequence": "contig_1", "start": 56289, "stop": 57224, "strand": "+", "frame": 3, "gene": null, "product": "DNA-directed RNA polymerase subunit alpha", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A288DGN6", "UniRef:UniRef90_A0A328EHC0" ], "nt": "ATGTTGAATATACAACACTTTATTGGAGTTCCGAAGATTGCGTTCAGTCAATTGGATGATGGTGCTACCAGGTTTGAATTGAAATATGTTCCTAGAGGATTTGGGCATACGCTTGGAAATGCTCTCAGGAGGATAATCCTCGCCTATAATCTTGGTGGGGCAGTTACAGGATTGAAGATAAAAGGAGTTCCTCATGAATACGATATTATTAGTGGGGTGAAGGAAAATGTGATTAGTATTATGTTAAACTTCAAGAAACTTCGCTTTAAAATCAATGAAAATGCTGATACCCTTCAGCGAATCTCTCAGAGATTTAAAGGGATCGGGAATTATAGTGCTGCGGATTTAAAGTTGCCTTCAGGAATTGAGCTCTTGAATGGTGAGGAACATTTGTTTGAGATTACTGATAGTTCGGTAGAACTTAATATGGAAGTGAGAATCGAGAAGGGATATGGTTATTATAGTTTGGATTATTTGAGACAAAGAGATGAGAAGGGGGATGGATCAGAAGTAAACTTGCTTTTGATTGATAATGATTTCTCTCTTGTAGATTATGTAAGGTATGATGTAGAAGAAGTGATTGAGGATTTTAGTGGAAGTACTAAGGATGCACTTAATCTTGAGATCAAGGCTAAGTATGTATCTATTGCTCCTCAGGAGATTGTAATGTTTGCGGGAGAGGTACTTGCAAGTTATGCTAAGCTCTTTGTGTTTGATAATGTATATATTGATAAGTCAGTTTTGGTTGATTATAATGATCTTAGTGAGGAGGCTGAAACTCCTATAGAAGAAGTTAGCTCTGTTAAGACGATGCCGATTGATGCACTTCCACTTAGTGAAAGGACAAGAAATGCATTGATCAAGAATAATATTCTCTATGTTGAGGATTTAGAAAGAAAGAAGAAATCAGAATTACTTGTGATGAAAGGAGTGTGA", "aa": "MLNIQHFIGVPKIAFSQLDDGATRFELKYVPRGFGHTLGNALRRIILAYNLGGAVTGLKIKGVPHEYDIISGVKENVISIMLNFKKLRFKINENADTLQRISQRFKGIGNYSAADLKLPSGIELLNGEEHLFEITDSSVELNMEVRIEKGYGYYSLDYLRQRDEKGDGSEVNLLLIDNDFSLVDYVRYDVEEVIEDFSGSTKDALNLEIKAKYVSIAPQEIVMFAGEVLASYAKLFVFDNVYIDKSVLVDYNDLSEEAETPIEEVSSVKTMPIDALPLSERTRNALIKNNILYVEDLERKKKSELLVMKGV", "aa_hexdigest": "400c8153710eccdd1a3c14342b2ea49c", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A328EHC0", "query_cov": 1.0, "subject_cov": 0.9367469879518072, "identity": 0.9940000000000001, "score": 597.0, "evalue": 3.52e-215, "valid": true, "product": "DNA-directed RNA polymerase subunit alpha", "uniref50_id": "UniRef50_A0A288DGN6" }, "pscc": { "uniref50_id": "UniRef50_A0A288DGN6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A288DGN6" ], "product": "DNA-directed RNA polymerase subunit alpha" }, "genes": [], "id": "NKOCPLAKPK_155", "locus": "GCA003260355_00113" }, { "type": "cds", "sequence": "contig_1", "start": 57325, "stop": 57711, "strand": "+", "frame": 1, "gene": null, "product": "50S ribosomal protein L17", "db_xrefs": [ "KEGG:K02879", "SO:0001217", "UniParc:UPI000DBFD0EC", "UniRef:UniRef100_A0A328EFV2", "UniRef:UniRef50_A0A288DGC1", "UniRef:UniRef90_A0A328EFV2" ], "nt": "ATGAGACATCAGCAGAATAAGCTCTTGCAATTGAACACTGGAGCAAAAAGTAAAACTGTTTTTATGAGAATGCAACTTTCTAGTTTGATTAGAGCTGGGAAATTGACTACGACTCCAAAGAGAGCTAAGGTTCTTAAAGCTTTTACTGATAGCTTCTTCTCTACTTTGTTAAGTTTTGATGCTAAGTATGATGAAAAGGGGGCTTTCAGAGAGGCAATTAGATATGTAAAATCTGTTGTTTATGGTGAAGCTGAAGGAAAAAAGGTAATTGAAACTTTGCTTCCAAAGTATAGAGAGGAGAAGAGAACTTCAGGTTTTACTACTTCTTATAAGCTTGGATTTAGAGTGGGGGATGCTGCTGCGAAAACCATGCTCAAGCTCGCATAG", "aa": "MRHQQNKLLQLNTGAKSKTVFMRMQLSSLIRAGKLTTTPKRAKVLKAFTDSFFSTLLSFDAKYDEKGAFREAIRYVKSVVYGEAEGKKVIETLLPKYREEKRTSGFTTSYKLGFRVGDAAAKTMLKLA", "aa_hexdigest": "1538ce0193f627c3a1e25452de601729", "start_type": "ATG", "rbs_motif": "TTATT", "ups": { "uniparc_id": "UPI000DBFD0EC", "uniref100_id": "UniRef100_A0A328EFV2", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBFD0EC", "UniRef:UniRef100_A0A328EFV2" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EFV2", "uniref90_id": "UniRef90_A0A328EFV2" }, "psc": { "uniref90_id": "UniRef90_A0A328EFV2", "product": "50S ribosomal protein L17", "uniref50_id": "UniRef50_A0A288DGC1", "kegg_orthology_id": "K02879" }, "pscc": { "uniref50_id": "UniRef50_A0A288DGC1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A288DGC1" ], "product": "50S ribosomal protein L17" }, "genes": [], "id": "NKOCPLAKPK_156", "locus": "GCA003260355_00114" }, { "type": "cds", "sequence": "contig_1", "start": 57752, "stop": 58210, "strand": "+", "frame": 2, "gene": "rplM", "product": "50S ribosomal protein L13", "db_xrefs": [ "GO:0003735", "GO:0005840", "GO:0006412", "KEGG:K02871", "SO:0001217", "UniParc:UPI000DC018D7", "UniRef:UniRef100_A0A660MQG6", "UniRef:UniRef50_A0A288GT76", "UniRef:UniRef90_A0A660MQG6" ], "nt": "ATGGAGTTTACGAAGAAAGATTTAAATAAAACCATCTGGATTAGGCAGACTGATCAAGAGAAAAATAGGAGATGGTATCGTGTAGATGCTACAGGAAAGACCCTTGGAAGATTGGCTGTGGATATTGCTAAGAAGCTTATGGGAAAGGATAGAGCTTATTATTCTGATTTTCGAGATGCAGGAAGTTTTGTTATCGTAGAAAATGTTGATCAGATTGCAGTGACAGGGAAAAAGCTTACTGATAAGGTTTATTATTCTTATAGCGGATATAAGGGGAATTTGAAGAGTATTACTCTTGGAGAGCTTCTCAAGAAAAATCCTGGTAAAGCTTTGTGGTATGCGGTAAGAGGTATGCTTCCAAAAAACAAGCTTAGAGATCCTAGAATTAAGAGATTAAAGCTTATTAAAGGAACGACTACGAAATACGATAATTTTAAACCAATTAACCTTTACAAGTAA", "aa": "MEFTKKDLNKTIWIRQTDQEKNRRWYRVDATGKTLGRLAVDIAKKLMGKDRAYYSDFRDAGSFVIVENVDQIAVTGKKLTDKVYYSYSGYKGNLKSITLGELLKKNPGKALWYAVRGMLPKNKLRDPRIKRLKLIKGTTTKYDNFKPINLYK", "aa_hexdigest": "8bd69055067e51635d10a66229b9b717", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000DC018D7", "uniref100_id": "UniRef100_A0A660MQG6", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DC018D7", "UniRef:UniRef100_A0A660MQG6" ] }, "ips": { "uniref100_id": "UniRef100_A0A660MQG6", "uniref90_id": "UniRef90_A0A660MQG6" }, "psc": { "uniref90_id": "UniRef90_A0A660MQG6", "gene": "rplM", "product": "50S ribosomal protein L13", "uniref50_id": "UniRef50_A0A288GT76", "kegg_orthology_id": "K02871", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_A0A288GT76", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A288GT76" ], "product": "Large ribosomal subunit protein uL13" }, "genes": [ "rplM" ], "id": "NKOCPLAKPK_157", "locus": "GCA003260355_00115" }, { "type": "cds", "sequence": "contig_1", "start": 58210, "stop": 58632, "strand": "+", "frame": 1, "gene": "rpsI", "product": "30S ribosomal protein S9", "db_xrefs": [ "GO:0003735", "GO:0005840", "GO:0006412", "KEGG:K02996", "SO:0001217", "UniParc:UPI000DBF44E5", "UniRef:UniRef100_A0A328EJ37", "UniRef:UniRef50_A0A7V4KHR1", "UniRef:UniRef90_A0A2W4IM16" ], "nt": "ATGACAAACAGAGAATACACTAATGCAGTTGGAAGAAGAAAGGAAACGACAGCAACTGTAAAACTCTATGCTAAGGGAGCAGGAAATATCCTTGTAAAGACAGTAACAGGGAAAGAATATACGCTTGCTGATTACTTTGGAGGAAATGTTTACCTCTATAAGAATGCAGTATTACCTTTTACTACGCTTGGTAATGATGTGCTCAAAAAATTTGATGCAGAAATTAAGATTGTGGGAGGGGGTATCGCTGGGCAGTCTGATGCAATCAAACTTGCTTTTTCAAGAGCTTTGGTAGAGTGGAATTCAGACCTCAGACCTACTTTGAAGCCTCATGGCCTTCTTAAAAGGGATCCTAGAATTAAGGAAAGAAAAAAGCCTGGTTTGAAAAAGGCGAGAAAGGCACCTACCTGGTCAAAGAGATAA", "aa": "MTNREYTNAVGRRKETTATVKLYAKGAGNILVKTVTGKEYTLADYFGGNVYLYKNAVLPFTTLGNDVLKKFDAEIKIVGGGIAGQSDAIKLAFSRALVEWNSDLRPTLKPHGLLKRDPRIKERKKPGLKKARKAPTWSKR", "aa_hexdigest": "982356141f60929d48b83455abf9ac9e", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000DBF44E5", "uniref100_id": "UniRef100_A0A328EJ37", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBF44E5", "UniRef:UniRef100_A0A328EJ37" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EJ37", "uniref90_id": "UniRef90_A0A2W4IM16" }, "psc": { "uniref90_id": "UniRef90_A0A2W4IM16", "gene": "rpsI", "product": "30S ribosomal protein S9", "uniref50_id": "UniRef50_A0A7V4KHR1", "kegg_orthology_id": "K02996", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_A0A7V4KHR1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7V4KHR1" ], "product": "Small ribosomal subunit protein uS9" }, "genes": [ "rpsI" ], "id": "NKOCPLAKPK_158", "locus": "GCA003260355_00116" }, { "type": "tRNA", "sequence": "contig_1", "start": 58859, "stop": 58930, "strand": "+", "gene": "trnR", "product": "tRNA-Arg(cct)", "amino_acid": "Arg", "anti_codon": "cct", "score": 47.6, "nt": "GCTCCTGTAGTTCAATGGATAGAATAGGCCCGTCCTAAGGGTTAGATGCTGGTTCGATTCCGGCCAGGAGTA", "db_xrefs": [ "SO:0001036" ], "anti_codon_pos": [ 58862, 58864 ], "id": "NKOCPLAKPK_8", "locus": "GCA003260355_00117" }, { "type": "cds", "sequence": "contig_1", "start": 59026, "stop": 59280, "strand": "+", "frame": 1, "gene": "rpsO", "product": "30S ribosomal protein S15", "db_xrefs": [ "GO:0003735", "GO:0005840", "GO:0006412", "SO:0001217", "UniParc:UPI000DBFD4F5", "UniRef:UniRef100_A0A328EFS0", "UniRef:UniRef50_A0A660MXH2", "UniRef:UniRef90_A0A328EFS0" ], "nt": "ATGGCAAAAACAACACAGGAAAATGTATTTCAAAGACACGATAAAGATACCGGGTCTCCAGAAATGCAAATCGGGATTTTATCTGATGAAATCGCAAAGCTTCAGGCGCATGTGGCTGCAAATGCAAAGGACTATGATGCTAAGAGAAGTCTTTTGAAGAAGGTAGCAAAGAGAAGAAGCTTTTTGAGATATCTCAAAGGGAAAGATCTTGATGCCTATACAAAAATCTCAAAAAAAATCGGACTAAAAGTGTAG", "aa": "MAKTTQENVFQRHDKDTGSPEMQIGILSDEIAKLQAHVAANAKDYDAKRSLLKKVAKRRSFLRYLKGKDLDAYTKISKKIGLKV", "aa_hexdigest": "18d1081fca6b481a700f7f01a6cf6153", "start_type": "ATG", "rbs_motif": "TTTA", "ups": { "uniparc_id": "UPI000DBFD4F5", "uniref100_id": "UniRef100_A0A328EFS0", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBFD4F5", "UniRef:UniRef100_A0A328EFS0" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EFS0", "uniref90_id": "UniRef90_A0A328EFS0" }, "psc": { "uniref90_id": "UniRef90_A0A328EFS0", "gene": "rpsO", "product": "30S ribosomal protein S15", "uniref50_id": "UniRef50_A0A660MXH2", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_A0A660MXH2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MXH2" ], "product": "Small ribosomal subunit protein uS15" }, "genes": [ "rpsO" ], "id": "NKOCPLAKPK_159", "locus": "GCA003260355_00118" }, { "type": "cds", "sequence": "contig_1", "start": 59433, "stop": 60491, "strand": "+", "frame": 3, "gene": null, "product": "S1 motif domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EJM4", "UniRef:UniRef90_A0A328EJM4" ], "nt": "ATGGCTAAAAAAGCTATTGCCCCAGCGGCAAGCCAATTTACCGTTCCTGTAATCAAGGAAGGGCAAAAGGTAAAAGGAACCATCCTTAAAGAAATCGAGAACGGAGTTCTCGTAAACTGTGAAAATGGTGCATTTACTGGAGTGATTCTTTCCAAAGAAGCGAAAGAACTCAAAAGATCTCAGTTTGATCTTAATCCATCTACTGAACTTGAGCTTGAAATCATCAATCCTGCAGTAAGACATGAAGATGGATACTATATTGTATCAGTTACAAGACTTCTCCAATACGATGTATGGAAGTCAGTGATCGCAAAGTTTGAAAAAGATGAAATTATTACTGTGGTTCCTACCGAGGCAAATCTTGGAGGACTTTTGATCGATATGCACGGTATTAAGGGATTCATCCCATTATCTCAATTGGCTCCTATTCACTATCCTAGAGTAGAAGATGGAGATCAAGATGCAATCTTTGATAAACTTCTTGAACTTATCGGACAGGAAATCAAAGTAAGAGTGATCAATATTGATGAAGAGGACAGAAGAATCATCCTTTCTGAAAGAGAGGCGCTCAAAGAAGAAAGAGAAAAGGTGCTTGCTGAACTTGAAGTTGGAAAGATCTTTGATGGTGTGGTTTCTGGAGTTTCTTCTTACGGACTTTTCGTGACTATCGGAGGAACTGTTGAAGGACTTGTGCATATTTCAGAAATTACTTACGGACACGTGAACGATATTGATAAACTCGGACACATTGGAGACAAGATGCAAGTTAAAGTGATCGGTCTTGAAAATGGAAAGATCTCACTCTCTAGTAAACAGCTCAAGGGAGATCCATGGGAGGAACTTCCAAAGAGATACCAGGTTGGAGATATTATTGAAGGAGAAATCGTGAGATATGTTCCTTATGGAGTATTCGTGAGAGTATATGAAGATATCAACGGACTCGTGCATTTGAGCGAGCTTTCTCAGAAATCAGTGAATAATCCAAACGAAATCGTGAAGCTTGGACAGATCGTGAAAGCAAAATTGATCCTCATTGATCCAAAAAATAGAAAAATCTGA", "aa": "MAKKAIAPAASQFTVPVIKEGQKVKGTILKEIENGVLVNCENGAFTGVILSKEAKELKRSQFDLNPSTELELEIINPAVRHEDGYYIVSVTRLLQYDVWKSVIAKFEKDEIITVVPTEANLGGLLIDMHGIKGFIPLSQLAPIHYPRVEDGDQDAIFDKLLELIGQEIKVRVINIDEEDRRIILSEREALKEEREKVLAELEVGKIFDGVVSGVSSYGLFVTIGGTVEGLVHISEITYGHVNDIDKLGHIGDKMQVKVIGLENGKISLSSKQLKGDPWEELPKRYQVGDIIEGEIVRYVPYGVFVRVYEDINGLVHLSELSQKSVNNPNEIVKLGQIVKAKLILIDPKNRKI", "aa_hexdigest": "b2f03827b5f5915a02430b47bd69a432", "start_type": "ATG", "rbs_motif": "TTATT", "psc": { "uniref90_id": "UniRef90_A0A328EJM4", "query_cov": 1.0, "subject_cov": 0.8956743002544529, "identity": 0.997, "score": 676.0, "evalue": 1.02e-244, "valid": true, "product": "S1 motif domain-containing protein", "uniref50_id": "UniRef50_A0A328EJM4" }, "pscc": { "uniref50_id": "UniRef50_A0A328EJM4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EJM4" ], "product": "S1 motif domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_160", "locus": "GCA003260355_00119" }, { "type": "cds", "sequence": "contig_1", "start": 60498, "stop": 60614, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGGATTTGGAACCAAAATCAGCTGATACCGAAGACAAGCCAGCGAAAAGAACTCCAAAAACAAAAGGAAGTCTTGAAGACGTAGTAAAGAAGATTGAAGAAAGTGAAGAATAG", "aa": "MKDLEPKSADTEDKPAKRTPKTKGSLEDVVKKIEESEE", "aa_hexdigest": "4dd49168d17b57bf45011a11681bb360", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4287.754099999999, "isoelectric_point": 5.07398281097412 }, "id": "NKOCPLAKPK_161", "locus": "GCA003260355_00120" }, { "type": "cds", "sequence": "contig_1", "start": 60905, "stop": 61006, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAGAAATTATCAAAGCACAAGGAGGAAATCCAAAAGTAGATTCTGAAACCCTACCTTTAGGATCTCATACGTTCGATATTATCGCAGAGAAGGATTGA", "aa": "MQEIIKAQGGNPKVDSETLPLGSHTFDIIAEKD", "aa_hexdigest": "07b01db8f4cc32ccce30f3d6511f4da8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3582.985600000001, "isoelectric_point": 4.696458244323729 }, "id": "NKOCPLAKPK_162", "locus": "GCA003260355_00121" }, { "type": "cds", "sequence": "contig_1", "start": 61022, "stop": 61246, "strand": "-", "frame": 3, "gene": null, "product": "Pyrimidine nucleoside phosphorylase C-terminal domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A351Y5K9", "UniRef:UniRef90_A0A328EFT2" ], "nt": "ATGAAAATGTCTGTTCAGATTACGGAAGCCAATGAAGTTATTGGAAACGGAGTAGGAGCAGTGATGCAAGTGCGTGAAGTGCTCAGAATTTTGCAGCAACATCCAGACAGAGCTATGGATCTTGAGAATAAGGTCATCTTCCTAGCTACCAAACTCATTGAAGAGGTCGGACTAGCAAAAGGGAAAGAAGCAAAAAAAATAGCAACCAACCAGCTTATTTCTTGA", "aa": "MKMSVQITEANEVIGNGVGAVMQVREVLRILQQHPDRAMDLENKVIFLATKLIEEVGLAKGKEAKKIATNQLIS", "aa_hexdigest": "9760174b56f47467c7624fa3b1bf1420", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 60719, "stop": 61246, "observations": { "mutation": [ 60736, 60805, 60907, 61024 ], "stop": [ 60736, 60805, 60907, 61024 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EFT2", "query_cov": 1.0, "subject_cov": 0.4, "identity": 1.0, "score": 139.0, "evalue": 1.52e-40, "gene_start": 1, "gene_end": 74, "reference_start": 1, "reference_end": 74, "reference_sequence": "MKMSVQITEANEVIGNGVGAVMQVREVLRILQQHPDRAMDLENKVIFLATKLIEEVGLAKGKEAKKIATNQLISGAAWKKMQEIIKAQGGNPKVDSETLPLGSHTFDIIAEKDGVIKMIDLHDINAVCRKLGCPVVDQAGLYLYKKTGDKVKKGEVVCRLYAQDEINLNLGKKRREERSPIQYTN" }, "paralog": false, "identity": 0.9771428571428571, "score": 330.0, "evalue": 4.77e-118, "description": "Internal stop codon at 60736, 60805, 60907, 61024. Nonsense mutation around 60736, 60805, 60907, 61024.", "psc": { "uniref90_id": "UniRef90_A0A328EFT2", "product": "Pyrimidine nucleoside phosphorylase C-terminal domain-containing protein", "uniref50_id": "UniRef50_A0A351Y5K9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EFT2", "UniRef:UniRef50_A0A351Y5K9" ] }, "pscc": { "uniref50_id": "UniRef50_A0A351Y5K9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A351Y5K9" ], "product": "Pyrimidine nucleoside phosphorylase C-terminal domain-containing protein" } }, "id": "NKOCPLAKPK_163", "locus": "GCA003260355_00122" }, { "type": "cds", "sequence": "contig_1", "start": 61261, "stop": 61431, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCAATATCCGCTTTCTATGCAATCTAGAGCTAAGGTCGTGAGTTCAATTATGGCGAAGAAATATGCTATGGGAGTGACCCATTCTTTGATTGATATTCCTGTTGGTCCAACTGCTAAGGTAAGCTCTATGAAAGAAGCAAAAGACTGGAAAAAAAGTTTGAATATGTAG", "aa": "MQYPLSMQSRAKVVSSIMAKKYAMGVTHSLIDIPVGPTAKVSSMKEAKDWKKSLNM", "aa_hexdigest": "6ac55a2776034fbdd5e11875ecbb83e2", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6200.365300000001, "isoelectric_point": 9.998482322692869 }, "id": "NKOCPLAKPK_164", "locus": "GCA003260355_00123" }, { "type": "cds", "sequence": "contig_1", "start": 61474, "stop": 62259, "strand": "-", "frame": 1, "gene": null, "product": "Glycosyl transferase family 3 N-terminal domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EJ44", "UniRef:UniRef90_A0A328EJ44" ], "nt": "ATGTTCAAATCTCATAAATCCAAAACTACAAAAAAAGACTTTTTAGTTTTTAAAGAAACCTCAGAATCCTATTATCCAGCAAAAGTTCAGCTTCTAGATATTAAAGATGGAGAAAGACTGATTGTCCTCCTCAATCCAAAACAAGCAATGGCATTTGGGATCAATCTCAATAATAAAGTCCAGCTCACCAAAACAAACGGAGAACATATCGTAGCTGACGTTTCTCTTTCAGAGGCAATCCCAACAGGGGAAGTTGCAATCTATGCAGACATTATTGATAAAATCTCCCTAAAAAATGATGAACTCATCGCAGTTTCTCTCGCAGAAAGTTCGAACGCTTCCTACGAAGCAATCAGAAAAAAAATGAGAGGAGAAAACATTAGTTATGATGAGATGTTTGCAATTATCAAAGATATTTCAGAGAATAAGCTTGATGACACAATGATGACTTACTATGTCGCTTCAAGTTTTTTCTATCCAACGACGGATGAAGAGATGTATCAGACCGCAAAAGCGATGGCAGAATGTGGGGTAATGTTCAAATATCCCAAAGGGGAAATTATCGCAGATAAGCACTGTATCGGTGGAGTCCCAGGAAATGAAACGACAATGATTCTGATTCCCCTCATCGCAAGCCTCGGTATCAAAATTCCAAAAAACTTCTCCAAATCCATCACTTCTCCAGCAGCAACCGGGGAATGTGTAAATGTGCTCATGAATATCAACTTCAACAAAGAAGGGATAGAAAATCTGGTAAAAGATCAGAATTGTTGCCTTGTTCGATGA", "aa": "MFKSHKSKTTKKDFLVFKETSESYYPAKVQLLDIKDGERLIVLLNPKQAMAFGINLNNKVQLTKTNGEHIVADVSLSEAIPTGEVAIYADIIDKISLKNDELIAVSLAESSNASYEAIRKKMRGENISYDEMFAIIKDISENKLDDTMMTYYVASSFFYPTTDEEMYQTAKAMAECGVMFKYPKGEIIADKHCIGGVPGNETTMILIPLIASLGIKIPKNFSKSITSPAATGECVNVLMNINFNKEGIENLVKDQNCCLVR", "aa_hexdigest": "b98fc11083acef74de3ceda1f7ed04d4", "start_type": "ATG", "rbs_motif": "TTTTT", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 61261, "stop": 62259, "observations": { "mutation": [ 61476 ], "stop": [ 61476 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EJ44", "query_cov": 1.0, "subject_cov": 0.786144578313253, "identity": 1.0, "score": 509.0, "evalue": 5.18e-181, "gene_start": 1, "gene_end": 261, "reference_start": 1, "reference_end": 261, "reference_sequence": "MFKSHKSKTTKKDFLVFKETSESYYPAKVQLLDIKDGERLIVLLNPKQAMAFGINLNNKVQLTKTNGEHIVADVSLSEAIPTGEVAIYADIIDKISLKNDELIAVSLAESSNASYEAIRKKMRGENISYDEMFAIIKDISENKLDDTMMTYYVASSFFYPTTDEEMYQTAKAMAECGVMFKYPKGEIIADKHCIGGVPGNETTMILIPLIASLGIKIPKNFSKSITSPAATGECVNVLMNINFNKEGIENLVKDQNCCLVRGGGLDLAPADDKLIKVQYPLSMQSRAKVVSSIMAKKYAMGVTHSLIDIPVGPTAKVSSMKEAKDWKKSLNM" }, "paralog": false, "identity": 0.9969879518072289, "score": 649.0, "evalue": 1.67e-238, "description": "Internal stop codon at 61476. Nonsense mutation around 61476.", "psc": { "uniref90_id": "UniRef90_A0A328EJ44", "product": "Glycosyl transferase family 3 N-terminal domain-containing protein", "uniref50_id": "UniRef50_A0A328EJ44", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EJ44", "UniRef:UniRef50_A0A328EJ44" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EJ44", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EJ44" ], "product": "Glycosyl transferase family 3 N-terminal domain-containing protein" } }, "id": "NKOCPLAKPK_165", "locus": "GCA003260355_00124" }, { "type": "cds", "sequence": "contig_1", "start": 62514, "stop": 63116, "strand": "+", "frame": 3, "gene": null, "product": "DEAD/DEAH box helicase family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002ADEC4CA" ], "nt": "ATGTTCTCAGAGGCTGATACGAGATCTAAATTCATCGACCCCAAACTTTATGCTCAAGGTTGGAAAGAGGAATATATCGTTAGAGAACGGTCTTTTACTGATGGGCGTAAACTCCCCGGAGGAAAAAGATCAGCTCCACTTTTTCTTGATTATCTTCTCAAGATGTATGGACTAAATCTCGCGATTATTGAGGCGAAGAAATATGGTTCCCTTGTGACAGAAGGACTTGAGCAAGTTAAAAATTATGGAAAGAAACTCGGGGTGAGGTGGGTCTATACTTCCAATGGAGAAGAGATTTATGAACGAGATATGCAGGAGGGGGATGGAAAACTAATCTCATCTTTTCCAAGCCCTGAGGAACTCTATGAAAGATATACGGATGAGCATGCTGTTCTTAGGCAACAGCTTTTATCTGTTCCTTATTTTCTTGATAGTGATAAAAAGCCTCGTTATTATCAGGATTTAGCGATTACTAGAGCATTGTCGGCTTTAGCAGAGGGTAAGGAAAGAATCTTACTTACGCTTGCAACGGGGACTGGAAAAACCTTTATTGCATTTCAGATTGTTTACAAGCTTCTCCAAGCAAAATGGTCAAAAAGCTGA", "aa": "MFSEADTRSKFIDPKLYAQGWKEEYIVRERSFTDGRKLPGGKRSAPLFLDYLLKMYGLNLAIIEAKKYGSLVTEGLEQVKNYGKKLGVRWVYTSNGEEIYERDMQEGDGKLISSFPSPEELYERYTDEHAVLRQQLLSVPYFLDSDKKPRYYQDLAITRALSALAEGKERILLTLATGTGKTFIAFQIVYKLLQAKWSKS", "aa_hexdigest": "c28c91884559e632a71ac30bc9a4740b", "start_type": "ATG", "rbs_motif": "TTATT", "psc": { "uniref90_id": "UniRef90_UPI002ADEC4CA", "query_cov": 0.995, "subject_cov": 0.868995633187773, "identity": 0.523, "score": 205.0, "evalue": 1.35e-63, "valid": false, "product": "DEAD/DEAH box helicase family protein", "uniref50_id": "UniRef50_UPI002ADEC4CA" }, "pscc": { "uniref50_id": "UniRef50_UPI002ADEC4CA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002ADEC4CA" ], "product": "DEAD/DEAH box helicase family protein" }, "genes": [], "id": "NKOCPLAKPK_166", "locus": "GCA003260355_00125" }, { "type": "cds", "sequence": "contig_1", "start": 63144, "stop": 63917, "strand": "+", "frame": 3, "gene": null, "product": "DEAD/DEAH box helicase family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00301548AD" ], "nt": "TTGGCGGATAGAAATATTTTGATCGATCAGGCACTTAATACTTTTAATCCTATAGAAAAAGACTGCAAGAGAATCAAAGGATCAGAAATAAAAAAAGCGCTTGGGAAAGTGCCCACTAGTGCAAATGTGTTTTTCTCGATTTATCAAGCTTTGATTTCGGGGACGGATGAGGAGACTGAAATTATAGAAGAAACTTTAGTTGGTAGTGAGGCTTTGGAGAGTGGATATTACCTCCAGTATGATCCAGATTTTTTTGATCTTATTATTATTGATGAATGTCATAGGGGAGGAACAAAGAATGATGGGAACTGGGCAGAAATCCTTAAATATTTTTCATCAGCAGTGCATCTTGGGATGACAGCCACACCAAAGAATCAGGATAATATCAATACTTATGCTTATTTTGGTGAGCCAGTTTATCAATATTCACTCAAAGAAGGAATAGAAGACGGATTTCTTACTCCATATAAAGTCAAAAGAATCCGGACCAATCTTGATGAATTGGTAATAGATGATAAAGTCGAGATTCTCGGTGGAAATAAAGAAAAAGATTTGTATGAGCTCAAAGATTTTGATAGAAAAATTATCGTTCCTGAGAGGATAGATCTCATTGCAAGAACAATCCTAGAACATATCAAGCCTCATGAAAAAACGATCATTTTCTGTGTGGATCAGGAGCATGCTGCACTGATGAGAGATGCGATCAATAAACATAAAACCGTGCGTGATGGAAATTACTGCGTGAGAGTGACGAGTAATGAAGGAGAAATCTGA", "aa": "MADRNILIDQALNTFNPIEKDCKRIKGSEIKKALGKVPTSANVFFSIYQALISGTDEETEIIEETLVGSEALESGYYLQYDPDFFDLIIIDECHRGGTKNDGNWAEILKYFSSAVHLGMTATPKNQDNINTYAYFGEPVYQYSLKEGIEDGFLTPYKVKRIRTNLDELVIDDKVEILGGNKEKDLYELKDFDRKIIVPERIDLIARTILEHIKPHEKTIIFCVDQEHAALMRDAINKHKTVRDGNYCVRVTSNEGEI", "aa_hexdigest": "ad3a9ee876bdde698c78fdb7d2ce9163", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001F10FF16", "query_cov": 1.0, "subject_cov": 0.9448529411764706, "identity": 0.5529999999999999, "score": 302.0, "evalue": 1.93e-100, "valid": false, "product": "DEAD/DEAH box helicase family protein", "ec_ids": [ "3.1.21.3" ], "uniref50_id": "UniRef50_UPI00301548AD", "kegg_orthology_id": "K01153" }, "pscc": { "uniref50_id": "UniRef50_UPI00301548AD", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00301548AD" ], "product": "DEAD/DEAH box helicase family protein" }, "genes": [], "id": "NKOCPLAKPK_167", "locus": "GCA003260355_00126" }, { "type": "cds", "sequence": "contig_1", "start": 63984, "stop": 64871, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTCACAACAGGAGTAGATGCCAAGAATGTCAGAAATATCGTTCTGCTCCGCAGTATTGGAAGTATGGTCGAGTTTAAGCAGATTATTGGTCGTGGGACAAGAGTCTTTGAGGGGAAGGATTATTTTACAATTATTGATTTTACTGGAGCAACGAATCTTTTTTATGATGAGAAGCGAGATGGTCCGATCTCTACAGTGTCTCCAGAAGACCAAGCAAAAGAGTCAAAAAAAGAAAAACATCAGACTTTTGATCCTGTTTCTTCTGTTGATTCTGAGGAGCCTCATATTCCAAAGCAGAAACTCAAAGTCAAACTCCTGGATAATAGAGAGCTCAAAATCATCAATATTGAAACCAGATATATCGGAGAGGATGGGAAACCTTTAACAGCAGAACAATATCTCTTGACTCTTGTTGGTAAGCTTCCAGCACTGTATCAGTCAGAAGAGCAGCTGAGAACCCTTCGATCTGATCCCAAAACAAGAGAAGATTTGCTCCTAGAGCTTAGGAAAATTGGACTGGATGAAGAGCATTTTAGTTTACTTCAGGCGATGTTTGAAGCTCCAGAGAGTGATCTTTTTGATGTCTTGACCTATCTAAGTTATGGTGAGGAAATGAAGACAAGAACTGAAAGAGTTGGTCGAGTGTTAGAGCAATCCTTGATGAAAAAAATTGAAAATCTGAGTGCAAAAGCTTTTTTAGAGTATCTCTTAGCATATTATCAGGAGCATGGAAGTACGGAACTCGTGCAGTCCAAGATGGGAGAACTGATAAAGCTTTATGGTCGTGGTCAGATCAATATTGTGGATTTTACAAAGGCCTTTGGTGGGCAAGAGGTATTAATGCAGGTATGGAAGGACTTGCAGAGGGAGTTATTTAGGATTTAG", "aa": "MLTTGVDAKNVRNIVLLRSIGSMVEFKQIIGRGTRVFEGKDYFTIIDFTGATNLFYDEKRDGPISTVSPEDQAKESKKEKHQTFDPVSSVDSEEPHIPKQKLKVKLLDNRELKIINIETRYIGEDGKPLTAEQYLLTLVGKLPALYQSEEQLRTLRSDPKTREDLLLELRKIGLDEEHFSLLQAMFEAPESDLFDVLTYLSYGEEMKTRTERVGRVLEQSLMKKIENLSAKAFLEYLLAYYQEHGSTELVQSKMGELIKLYGRGQINIVDFTKAFGGQEVLMQVWKDLQRELFRI", "aa_hexdigest": "8d85f4b4dce3b3cf2c8a39e56c16e716", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 33980.564700000024, "isoelectric_point": 5.51232509613037 }, "id": "NKOCPLAKPK_168", "locus": "GCA003260355_00127" }, { "type": "cds", "sequence": "contig_1", "start": 64889, "stop": 65023, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTAATGAAATGGGTAAACAATATTTTCAATATGTTATGGGGCTTATAAAAGCTCTAAGTTTTTTTAATCAATATAGAGATGGTATTATGTATTATGATAATAAACAATTGTTTTTATCTGATATCTTTTGA", "aa": "MANEMGKQYFQYVMGLIKALSFFNQYRDGIMYYDNKQLFLSDIF", "aa_hexdigest": "932ddd95f5cc306bccddfaf90ef8e0d8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5343.1362, "isoelectric_point": 5.918496513366699 }, "id": "NKOCPLAKPK_169", "locus": "GCA003260355_00128" }, { "type": "cds", "sequence": "contig_1", "start": 65972, "stop": 66079, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EHD0", "UniRef:UniRef90_A0A328EHD0" ], "nt": "ATGAAAAATACAGAACAAAAATTGTTAGAATATGTAGAGGGATTTATTGATAAGAACAAACTTTCTTTGGAAGAAAGAACCTACTATATAAATCTTTTAGTAGAATGA", "aa": "MKNTEQKLLEYVEGFIDKNKLSLEERTYYINLLVE", "aa_hexdigest": "530cf1a6c08e5f103110865422037db2", "start_type": "ATG", "rbs_motif": "TTATT", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 65972, "stop": 66175, "observations": { "mutation": [ 66077, 66152 ], "stop": [ 66077, 66152 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EHD0", "query_cov": 1.0, "subject_cov": 0.5223880597014925, "identity": 1.0, "score": 71.2, "evalue": 1.24e-15, "gene_start": 1, "gene_end": 35, "reference_start": 1, "reference_end": 35, "reference_sequence": "MKNTEQKLLEYVEGFIDKNKLSLEERTYYINLLVEGNSSIYDKEYMKVYFSYLVDRLLLEGKITLDI" }, "paralog": false, "identity": 0.9701492537313433, "score": 127.0, "evalue": 3.52e-40, "description": "Internal stop codon at 66077, 66152. Nonsense mutation around 66077, 66152.", "psc": { "uniref90_id": "UniRef90_A0A328EHD0", "uniref50_id": "UniRef50_A0A328EHD0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EHD0", "UniRef:UniRef50_A0A328EHD0" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EHD0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EHD0" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 4264.8472, "isoelectric_point": 4.92949810028076 }, "id": "NKOCPLAKPK_170", "locus": "GCA003260355_00129" }, { "type": "cds", "sequence": "contig_1", "start": 66194, "stop": 66307, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACCCTACTTATTTTATTAAAAGCATTAGTATATTTGGATATTTAGAAGGTAAGTTATTACTAAATTCTAAGACAACTTTTTCTACTAATTTAGTTAATGCGATAATTTGA", "aa": "MNPTYFIKSISIFGYLEGKLLLNSKTTFSTNLVNAII", "aa_hexdigest": "06d5d478f56d37b3a49f64a7c0f80fa0", "start_type": "ATG", "rbs_motif": "TTTTTA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4152.847900000001, "isoelectric_point": 9.400086784362792 }, "id": "NKOCPLAKPK_171", "locus": "GCA003260355_00130" }, { "type": "cds", "sequence": "contig_1", "start": 66587, "stop": 66832, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATGAGTTTTCAAATTTATTTCATTTTTACGATAGTCTATTTAGTTTGGCTACTCTACCTAAAGAAATAAAAGCAAAGAATATTGTAAAACTTCAATTGAAAGATTTGTTTTTTTCTTTTCGATTTTTAAATAAATATAATTACGGGAATATTTTTTTACCAAGAAATATATATGGGAATATATCAATTACAGCAACACATAGAACTTTTTTCCCTATATATTTATATTTACTATTGCCGTGA", "aa": "MDEFSNLFHFYDSLFSLATLPKEIKAKNIVKLQLKDLFFSFRFLNKYNYGNIFLPRNIYGNISITATHRTFFPIYLYLLLP", "aa_hexdigest": "8061758abd43fe44b692ac49cd7adf99", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9707.251599999989, "isoelectric_point": 9.521674156188965 }, "id": "NKOCPLAKPK_172", "locus": "GCA003260355_00131" }, { "type": "cds", "sequence": "contig_1", "start": 66989, "stop": 67339, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTACAGAGGAAGAAATTTATAAAAATGAAGTTAAAAGTATTGTTCAAATACAGATTGAAGTTGATGCAATTATTGATAAGCTATCTATATTGGAAATTAATCTTTTAAAAATTGAGCAATATTTAGCATTTACTGGTTCTAAAATTAATTTGACGGTCTATCAAAAAGGATATTGGCTTGATAGGAAAAATGAATTAAAAAGCCTTATTGATAGGTATAGTAATGAATTAAGAGAGAAAAAAAATAGTATAGAAAAATTAAAAAGAGACAATAATAAATCTTTGTTAAAAACTGGATTCTATTCTGGTGTTTTGGATTTTGTTGACAAGGAGGTTGTAACAGAATGA", "aa": "MFTEEEIYKNEVKSIVQIQIEVDAIIDKLSILEINLLKIEQYLAFTGSKINLTVYQKGYWLDRKNELKSLIDRYSNELREKKNSIEKLKRDNNKSLLKTGFYSGVLDFVDKEVVTE", "aa_hexdigest": "6c386cdeb9fc3f78acddede2471b23db", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13667.558799999979, "isoelectric_point": 5.65470676422119 }, "id": "NKOCPLAKPK_173", "locus": "GCA003260355_00132" }, { "type": "cds", "sequence": "contig_1", "start": 67439, "stop": 67717, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTGTCTCAGGATAATCCAGATTTTCAAAAATTTAAAGATTTTTATAATGATGAAATAAAAAATGTAGGATTGGGTGTATTAGAAAAAGAATTGCAGGCTGAAAAATTTAGTATAGAAAAAGATTTGATAGGTCCAAAACTTAACGTGGATAATGGATTAAATATATTTGTGAATCTTATAATGATCAAATATTTGTATCTTTGTCTTAAAAAGAATACAAGATTTTTCCTTCCTCCACTTGTCATTGATAATACATTTGCTGATTTAAATTCATGA", "aa": "MLSQDNPDFQKFKDFYNDEIKNVGLGVLEKELQAEKFSIEKDLIGPKLNVDNGLNIFVNLIMIKYLYLCLKKNTRFFLPPLVIDNTFADLNS", "aa_hexdigest": "564b31ff11648f04d855d5b910e92c7f", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10674.277799999985, "isoelectric_point": 4.98173313140869 }, "id": "NKOCPLAKPK_174", "locus": "GCA003260355_00133" }, { "type": "cds", "sequence": "contig_1", "start": 68224, "stop": 68394, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATAAATAAAGAATTAGAGGAACAATTTGATATCCAAATTCAACTCATTCAATTATCAATAAAAGATTTTGATAAAGGAGATTTTCTTGCGGCTATGAACTTGGCAATAAGAATTAGGTTTTTAATACATGATACTAATAGGTCAGTTTCATTATTAACACAAATGTGA", "aa": "MINKELEEQFDIQIQLIQLSIKDFDKGDFLAAMNLAIRIRFLIHDTNRSVSLLTQM", "aa_hexdigest": "549ecc6e2d0a598122a1db337f9987bb", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6579.621899999998, "isoelectric_point": 5.037946891784667 }, "id": "NKOCPLAKPK_175", "locus": "GCA003260355_00134" }, { "type": "cds", "sequence": "contig_1", "start": 70206, "stop": 70769, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DC004E3", "UniRef:UniRef100_A0A328EFU2", "UniRef:UniRef50_A0A328EFU2", "UniRef:UniRef90_A0A328EFU2" ], "nt": "ATGAAGCAACTAACAAAACTTATGATGACCAAGCAAACGGTCTTTAATATAGAGGAGCTTACTAGCTATCTTCAAATCCCTACATCAGCAGGCTTGAAAAGTTTTTTGCAAAGAGCAAAACAGTCAAAAGCTTTGCTTAATCCGATCAAAGGAATTTGGACTTTCCCGATATATGATCCTTTTGAGTTCTCATGCAAACTCAATTCTGATGCTTATATTTCTTGTGAAACGGTACTTTTTAGAGAAGGTATTTTCTTTCAGTTTTATGGGGATACTGTCAGCTCAGTTACCTTCAAATCAAGGAGTTATGATATTGATGGTCAGCATTTTATCTATTATCGTATTCAGCCAAAGATTGCTCAAAATCTGATCGGAATAAGAGATTATGATTGTTATCGTATGGCAACTCCTGAGAGAGCTTTGTGTGATTATATCTATCTTCATCCCAAGGCGAGTATTGATGCGCCAGAGTTGATCAATCAGAGAAGAATAGAAAAACTCTATCCATTCTATCCTAAAAAAACGGTTTTAAGAATTAAACAGCTTCTCAATGCTCAATTATAA", "aa": "MKQLTKLMMTKQTVFNIEELTSYLQIPTSAGLKSFLQRAKQSKALLNPIKGIWTFPIYDPFEFSCKLNSDAYISCETVLFREGIFFQFYGDTVSSVTFKSRSYDIDGQHFIYYRIQPKIAQNLIGIRDYDCYRMATPERALCDYIYLHPKASIDAPELINQRRIEKLYPFYPKKTVLRIKQLLNAQL", "aa_hexdigest": "c5028e4d431aff30d28d71087c1a2f1f", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000DC004E3", "uniref100_id": "UniRef100_A0A328EFU2", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DC004E3", "UniRef:UniRef100_A0A328EFU2" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EFU2", "uniref90_id": "UniRef90_A0A328EFU2" }, "psc": { "uniref90_id": "UniRef90_A0A328EFU2", "uniref50_id": "UniRef50_A0A328EFU2" }, "pscc": { "uniref50_id": "UniRef50_A0A328EFU2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EFU2" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 21942.298200000016, "isoelectric_point": 9.269087028503417 }, "id": "NKOCPLAKPK_176", "locus": "GCA003260355_00135" }, { "type": "cds", "sequence": "contig_1", "start": 70756, "stop": 71289, "strand": "+", "frame": 1, "gene": null, "product": "Nucleotidyl transferase AbiEii/AbiGii toxin family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EJ51", "UniRef:UniRef90_A0A328EJ51" ], "nt": "ATGCTCAATTATAATAAGCACAAAGAATTTATGTTCAAAATTCTTATTCGTATTTTTCAAAGTCCGCTTAAGGATGTACTTGCTTTCAAGGGAGGAACGCTGGCTTTCCTTTGCTATGGTTTACCGAGATTCTCTACAGATATTGATTTAGATTTACTAGATTATAGTAAGGAGCCAGATCTATTAGAGATAATGCAGGATATTTTGGAAGATATTGGAGAAATTAAGAATATGACTTTAGGAAAGGAGCTTCATCGTTGGATTTTTCGCTATGATCCATCGACTATGAATATCAAAGTAGAGGTCAATAAAAGACCGCTTGATGGCAATATTTATGAGCGAAAAACCATTGAGAATACTCAAGTATATTGTATGAGTCAGTCTTCTATGGTAGCAAATAAGATAGTTGCTCTTTATGAGAGAGCATATCCTAGAGATCTCTTTGATGTATATTATTTCCTTGCTCAGGATTTCCCTTGTGATAATGCAGTGATTGAGCTTAGGTCAGGGAAGTCTCTACACGAGCTTATCTGA", "aa": "MLNYNKHKEFMFKILIRIFQSPLKDVLAFKGGTLAFLCYGLPRFSTDIDLDLLDYSKEPDLLEIMQDILEDIGEIKNMTLGKELHRWIFRYDPSTMNIKVEVNKRPLDGNIYERKTIENTQVYCMSQSSMVANKIVALYERAYPRDLFDVYYFLAQDFPCDNAVIELRSGKSLHELI", "aa_hexdigest": "43051b99f39f99d04bfc48a3c97d707b", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 70756, "stop": 71445, "observations": { "mutation": [ 71287 ], "stop": [ 71287 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EJ51", "query_cov": 1.0, "subject_cov": 0.7729257641921398, "identity": 1.0, "score": 359.0, "evalue": 5.57e-125, "gene_start": 1, "gene_end": 177, "reference_start": 1, "reference_end": 177, "reference_sequence": "MLNYNKHKEFMFKILIRIFQSPLKDVLAFKGGTLAFLCYGLPRFSTDIDLDLLDYSKEPDLLEIMQDILEDIGEIKNMTLGKELHRWIFRYDPSTMNIKVEVNKRPLDGNIYERKTIENTQVYCMSQSSMVANKIVALYERAYPRDLFDVYYFLAQDFPCDNAVIELRSGKSLHELIGELQEIIPRYFLPNTVLIGLGELLTDTQKLRVKKHLVTETVALLGQYFLKNK" }, "paralog": false, "identity": 0.9956331877729258, "score": 457.0, "evalue": 6.81e-166, "description": "Internal stop codon at 71287. Nonsense mutation around 71287.", "psc": { "uniref90_id": "UniRef90_A0A328EJ51", "product": "Nucleotidyl transferase AbiEii/AbiGii toxin family protein", "uniref50_id": "UniRef50_A0A328EJ51", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EJ51", "UniRef:UniRef50_A0A328EJ51" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EJ51", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EJ51" ], "product": "Nucleotidyl transferase AbiEii/AbiGii toxin family protein" } }, "id": "NKOCPLAKPK_177", "locus": "GCA003260355_00136" }, { "type": "cds", "sequence": "contig_1", "start": 71478, "stop": 71732, "strand": "+", "frame": 3, "gene": null, "product": "Site-specific DNA-methyltransferase (adenine-specific)", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A837JCA8" ], "nt": "ATGCAAGCACAAATCAATCGTATTACTGATATTCTTCGCCGTGATGATGGAATCTCTGGGGCAATGCATTATACAGAGCAGATCAGCTGGATTCTTTTTCTCAAGTTTCTTGATGACTATGAATCAGCTCATGTATTAGAAAGTATGCTCAATGGTAAGGAATATACCAGATTACTTGCACCCGCCTATCAGCGATCCTCTCGAGCGGCTCCTAAAAAAGATGGAAAGTTAGATCATACGAAGGCTCTTGTTTGA", "aa": "MQAQINRITDILRRDDGISGAMHYTEQISWILFLKFLDDYESAHVLESMLNGKEYTRLLAPAYQRSSRAAPKKDGKLDHTKALV", "aa_hexdigest": "5c9b116ae634aad4ed9c4f8c291fefea", "start_type": "ATG", "rbs_motif": "TTTTT", "psc": { "uniref90_id": "UniRef90_UPI00299F6298", "query_cov": 0.8571428571428571, "subject_cov": 0.935064935064935, "identity": 0.597, "score": 86.3, "evalue": 1.41e-20, "valid": false, "product": "type I restriction-modification system subunit M N-terminal domain-containing protein", "uniref50_id": "UniRef50_A0A837JCA8" }, "pscc": { "uniref50_id": "UniRef50_A0A837JCA8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A837JCA8" ], "product": "Site-specific DNA-methyltransferase (adenine-specific)" }, "genes": [], "id": "NKOCPLAKPK_178", "locus": "GCA003260355_00137" }, { "type": "cds", "sequence": "contig_1", "start": 71907, "stop": 72062, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATTTTCAGTCGCAGTCAGATTTATTTGACCTTTCTAAAATTTATGAAGACCTTTTGCAAGGGATGGGGAATGATGGAGGAAATAGTGGGGAATACTATACTCCAAGAGCAGTGATTAAAGCCATGGTAGAGTGTCTTGATCCAAAGATTTGA", "aa": "MNFQSQSDLFDLSKIYEDLLQGMGNDGGNSGEYYTPRAVIKAMVECLDPKI", "aa_hexdigest": "d89c2c44342e6cdc3ff21febdc9ddbd0", "start_type": "ATG", "rbs_motif": "TTATT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5703.3690000000015, "isoelectric_point": 4.236858177185058 }, "id": "NKOCPLAKPK_179", "locus": "GCA003260355_00138" }, { "type": "cds", "sequence": "contig_1", "start": 72213, "stop": 72347, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGAGTAATGAATATGATTCTCCATGGTATCGAAAATCCTAATATTAACAAGAAAAATACGCTTGTAGACAATATTAGGGATTTTGAGGAAAAGGACGCTTATGATATTATCCTTGCAAATCCTCCCTTTTGA", "aa": "MGVMNMILHGIENPNINKKNTLVDNIRDFEEKDAYDIILANPPF", "aa_hexdigest": "abc3160aa6ea71268d4ce36375cf1747", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5058.764100000001, "isoelectric_point": 4.646496772766113 }, "id": "NKOCPLAKPK_180", "locus": "GCA003260355_00139" }, { "type": "cds", "sequence": "contig_1", "start": 72480, "stop": 72746, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTCAATACTTCCAATGCTTTTCAGGAAGTCAAAAAGCAGTTGCTTTCAGACTTTAATTTGCATTCTATAGTTTCTTTGCCTGCGGGGATTTTCTTACCGTATAGTGGAGTTAAGACCAACATTATCTTCTTTGACAAGACTAAAGCTACCGAGCAGATTCGATATTATGAGATTGACCTTGGTCGTAAATTGACTAAGAATAAGCCAATTACTTTTGAGGAGCTTCAAGGTATGATTTTAGCTTTCAAAAAAAGAGAAGTCTGA", "aa": "MFNTSNAFQEVKKQLLSDFNLHSIVSLPAGIFLPYSGVKTNIIFFDKTKATEQIRYYEIDLGRKLTKNKPITFEELQGMILAFKKREV", "aa_hexdigest": "aef15123a4a72725705d6b0fdb981fd8", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10203.78819999999, "isoelectric_point": 9.476159477233889 }, "id": "NKOCPLAKPK_181", "locus": "GCA003260355_00140" }, { "type": "cds", "sequence": "contig_1", "start": 72762, "stop": 72923, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGACATCTTCAGAGATCAAAGACTATGATCTTTCTGCAAAAAATCCTGCAAATATCAAAGAAATTATCCATAGAGCTCCAAATGAGATTATGGAAGATATTATTAGGACAAATGAAATAATTTTTGAGGGAACTAGTCAGCTTCGCACTTTGATTAAATAA", "aa": "MTSSEIKDYDLSAKNPANIKEIIHRAPNEIMEDIIRTNEIIFEGTSQLRTLIK", "aa_hexdigest": "103b294ab5fce6022695c5f3fcd98ec3", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6101.914699999997, "isoelectric_point": 5.187660789489745 }, "id": "NKOCPLAKPK_182", "locus": "GCA003260355_00141" }, { "type": "cds", "sequence": "contig_1", "start": 73426, "stop": 73653, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTGGCTGGGAAATTGGTATATATTTTTTCTGGGCTTTTTTACTTTTTGGCAGGAATGCATAGTTCTCCTTTGCTTCTTGTGCTAGCTGTTGTTTTGAATGGAATTGCGAGTTCTATGATGTTTACGACTTATCGTGTGCTTTATGGGAAGGGAACTCAGCATAGTAACCGCAGTAAGATTTTCGGTTTTTATTTCTCCAGTATTAATCTTGCATATGTAATCTGA", "aa": "MLAGKLVYIFSGLFYFLAGMHSSPLLLVLAVVLNGIASSMMFTTYRVLYGKGTQHSNRSKIFGFYFSSINLAYVI", "aa_hexdigest": "6eec7eb83457e00ff669f155ce1224a9", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8337.837799999998, "isoelectric_point": 9.90029697418213 }, "id": "NKOCPLAKPK_183", "locus": "GCA003260355_00142" }, { "type": "cds", "sequence": "contig_1", "start": 73657, "stop": 74133, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTTAGTGCTTTTCTTGTTTCCTATCTTGAACTTCCTTATATGTATCTTTTTATTATTATTTTTGCGTTGTTGTCTCTGCTTCAGGATGGAAAGATTCAGGATTTTATTAAGAAAAAATTTTCTAAATCTTGGAAAAAGTGGAACTTTCTTGTAAATTCTGTAGATACTGATGTTGACGTAGACTTGTGTAATATTAGGAAACTTTTTGGGAAAAGAGGGGTAATAGTTCGCTTTTCGAAGGAGGCAGTTTCTTTTGCTTCGTGGAAAAGGATGTTTTCTACTCTGAAATCGTATGGTCGATCAATGTATACTGCACTTTCTGCTCAGGCTTTGGTAAGTTTTATGAATTATGTGGGATTTTTGTTTATTCCGATTATTGCGAATGATAATAATCTTTCTCTTTCTGAAATTGCTATTTTGTTCGCTGTGATGAGGCTTCCTTACGTGATTAACGTTTTGATCGGAGGATTTTGA", "aa": "MLSAFLVSYLELPYMYLFIIIFALLSLLQDGKIQDFIKKKFSKSWKKWNFLVNSVDTDVDVDLCNIRKLFGKRGVIVRFSKEAVSFASWKRMFSTLKSYGRSMYTALSAQALVSFMNYVGFLFIPIIANDNNLSLSEIAILFAVMRLPYVINVLIGGF", "aa_hexdigest": "451e6979d158b5e864b306389320e33a", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18129.378500000003, "isoelectric_point": 9.679621696472168 }, "id": "NKOCPLAKPK_184", "locus": "GCA003260355_00143" }, { "type": "cds", "sequence": "contig_1", "start": 74329, "stop": 74565, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCTGGATTGCAGGAGTTTGCTAGTAGGATTGGAGAAATTATCGGCTCTTTGGTTTTTGGAATGATCGTTGCAAAAATTGGAATGGATGATTCTTTTATTGTTTTGGGAGGGGCTCTTGCGGTGTTAAGTATGTATCTTTTGGTAAAGAAGCTCATGAGAATAAAGACGAAAGATGGTGAAGCGAAAAAAGAGGTTGAAGCTCAGCAGCCTTTACCCTTTTTGCAGAATACTTAA", "aa": "MSGLQEFASRIGEIIGSLVFGMIVAKIGMDDSFIVLGGALAVLSMYLLVKKLMRIKTKDGEAKKEVEAQQPLPFLQNT", "aa_hexdigest": "e9b6d59847c22a594b60c0f933908ec2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8486.060499999996, "isoelectric_point": 8.035291099548338 }, "id": "NKOCPLAKPK_185", "locus": "GCA003260355_00144" }, { "type": "cds", "sequence": "contig_1", "start": 74674, "stop": 74895, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MSI2", "UniRef:UniRef90_A0A660MSI2" ], "nt": "ATGGGGAAAGTAGCTCTTCTAAAAAAAAATATTGGGATCTTGTTGCTCTCTCCCTTTGTTGCTTTTGGAATTTTTCGAGTTTCTCAGCAAGATTGGAGTAGGCTAACGGCGAGCGTTCTTGATATTTCAGAAATTGAAACGATTGAGAAACAAGGGCGAGATGTTGCATACAAAGTTCATGATCAGCTTTTTGAACTTTTTGGTTCAAAGCAGCTTCAATGA", "aa": "MGKVALLKKNIGILLLSPFVAFGIFRVSQQDWSRLTASVLDISEIETIEKQGRDVAYKVHDQLFELFGSKQLQ", "aa_hexdigest": "d10a87529706021a0bffad810347f7ec", "start_type": "ATG", "rbs_motif": "TTTTT", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 74674, "stop": 75186, "observations": { "mutation": [ 74893, 75073 ], "stop": [ 74893, 75073 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A660MSI2", "query_cov": 1.0, "subject_cov": 0.4294117647058823, "identity": 0.986, "score": 138.0, "evalue": 3.81e-40, "gene_start": 1, "gene_end": 73, "reference_start": 1, "reference_end": 73, "reference_sequence": "MGKVTLLKKNIGILLLSPFVAFGIFRVSQQDWSRLTASVLDISEIETIEKQGRDVAYKVHDQLFELFGSKQLQGGDALVFELVYNPEKLSLVLPESSGATFSVLENESGVFRAKLENLSHYMLNSDWIQIPFSGEEDQLVLSEAMLYRGEKSSLLKIGNLSSSTEHSLLP" }, "paralog": false, "identity": 0.9294117647058824, "score": 306.0, "evalue": 7.39e-109, "description": "Internal stop codon at 74893, 75073. Nonsense mutation around 74893, 75073.", "psc": { "uniref90_id": "UniRef90_A0A660MSI2", "uniref50_id": "UniRef50_A0A660MSI2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A660MSI2", "UniRef:UniRef50_A0A660MSI2" ] }, "pscc": { "uniref50_id": "UniRef50_A0A660MSI2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MSI2" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 8265.539499999999, "isoelectric_point": 8.086672401428224 }, "id": "NKOCPLAKPK_186", "locus": "GCA003260355_00145" }, { "type": "cds", "sequence": "contig_1", "start": 75249, "stop": 75407, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAACATTTGCGGTGGGAAGTCCTGATAGTTTTCAACTGGAGGTAAATCCTGCAACTTTTAGTGTGAATCAACCCGTTGATCTTACGGTAAAAGCAATTAAGAATGGGCAGATTATGAAGGATTATGAGGGAAGGCTTTTCCTAGAAATTGAGTGA", "aa": "MTTFAVGSPDSFQLEVNPATFSVNQPVDLTVKAIKNGQIMKDYEGRLFLEIE", "aa_hexdigest": "3bb3c5a128de69a904608067ba8a88cf", "start_type": "ATG", "rbs_motif": "TTTTT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5790.530799999998, "isoelectric_point": 4.5748796463012695 }, "id": "NKOCPLAKPK_187", "locus": "GCA003260355_00146" }, { "type": "cds", "sequence": "contig_1", "start": 75459, "stop": 75800, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGCTTTCTAACCAGGGAGTGAAAACCTATTCTAAAGGATTACAAGTAAAAAAGGCTGGAACTTTTGTCTTGAAAGTCGAGGATCTCGCTTCAGATTCAGCTACTGGAACGATTACGATTATTGTCTCTTCTGATGATCAACTTAACCTTAAAAATATTCAGCTTCTTTCTCCTCTTCAAAATGGGACAGAAACAGAGAGTTCTATGAGTGTGATGGCAAATGCTCCAGAATTATCAAACTCTCGTATTCAGGTTTATTTGAATGATCTAATGGTAAAAGAGGCAATGACTGATACTAATGGATTATTCAATGAAACCATTCCACTTCAAAAGGCGTGA", "aa": "MKLSNQGVKTYSKGLQVKKAGTFVLKVEDLASDSATGTITIIVSSDDQLNLKNIQLLSPLQNGTETESSMSVMANAPELSNSRIQVYLNDLMVKEAMTDTNGLFNETIPLQKA", "aa_hexdigest": "02b5e09e645c1754835f6ec94920c87d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12236.827199999989, "isoelectric_point": 4.986393928527831 }, "id": "NKOCPLAKPK_188", "locus": "GCA003260355_00147" }, { "type": "cds", "sequence": "contig_1", "start": 76026, "stop": 76277, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGATAAAGAAAAGGATGGGCTTTTTACTAAAAATGTTACGCTTACTACTACTGGAGAAATCATTGTGAATACAGAACTTATTGCTTCAGAACTTACTAAGAAGTATGATAAGGTATTGTCATTTTCTGTTAGAGATCATATTAGAATTGGTGAAGTGAGAATCAGTCTTAATCCTAATCTTGCTGGGACTTTAGATCTTTCTTGGGTAACTCTTGGAGGAGAGAGCTCTGATTATGCTGTAAAGTATTGA", "aa": "MDKEKDGLFTKNVTLTTTGEIIVNTELIASELTKKYDKVLSFSVRDHIRIGEVRISLNPNLAGTLDLSWVTLGGESSDYAVKY", "aa_hexdigest": "bffeadf88d2b655cabff872ecac4b586", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9220.381199999998, "isoelectric_point": 5.257856941223144 }, "id": "NKOCPLAKPK_189", "locus": "GCA003260355_00148" }, { "type": "cds", "sequence": "contig_1", "start": 76278, "stop": 76598, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAGAAGGATCAATTGATTGCTACAGCCTTTACTAAGAAACCTCAAGCAACTCTTTCAGGTTTTTCTTATGGGAAATCGTATTTTTTTCAAGTTTTGGCAACGACTCCTGAGCATGTCCCTGAAGGACTACCTTCTCAGGTTATTCAGTTTGATATGCCAATTTTATCAGGAACTGCGGCTACGGTTGATCCTCTAACAAGTGGCGGATTACCTTCTGATCTGGCTGATCCTGCAGATCCAGATTCTCATGATGTGGCGGTTTTGGATAAGCCGCTTTGTGTTGTCAAGAATATTCAGTTTTCTACTAAGAAGATTTGA", "aa": "MEKDQLIATAFTKKPQATLSGFSYGKSYFFQVLATTPEHVPEGLPSQVIQFDMPILSGTAATVDPLTSGGLPSDLADPADPDSHDVAVLDKPLCVVKNIQFSTKKI", "aa_hexdigest": "43ac7b3f892c33caba7702d949fc4d03", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11335.806499999986, "isoelectric_point": 4.847195243835448 }, "id": "NKOCPLAKPK_190", "locus": "GCA003260355_00149" }, { "type": "cds", "sequence": "contig_1", "start": 76635, "stop": 76916, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGAAAAATATCTGATTTATAGGTCTGAATATGAGGATGGAAGTGACAGGCAATTTGTTGGGGAATCTCTTGTTCCAAGATATGAATATCCTTTTGATAATACTGCGGAGGAGGATATTTATGCCTATTATGACGTAGAGGCTATTTGTAATGATGGTGAACAGCTTGTTTTGACTAAAGCAGAAAAGGTAAAGGTTGGTCCTTTTGAAGATATGTTGATGATTCTTGCTTGTACTTTCCTTATTTATTTGATGTATAGGCTTTATCACTATAATGCCTAG", "aa": "MEKYLIYRSEYEDGSDRQFVGESLVPRYEYPFDNTAEEDIYAYYDVEAICNDGEQLVLTKAEKVKVGPFEDMLMILACTFLIYLMYRLYHYNA", "aa_hexdigest": "aee17834fa33885c7b7d0c25a0bd2014", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11084.416199999994, "isoelectric_point": 4.308645820617676 }, "id": "NKOCPLAKPK_191", "locus": "GCA003260355_00150" }, { "type": "cds", "sequence": "contig_1", "start": 77246, "stop": 77662, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTCTGTTTATAGGAAAACACAGAAAAAAAATCAGACTTTACTTTCTCAATTTTTTGGTAATGAGGGTATCCATTTTTGTTCTACGTTTCAGAATGAGAGGATTCAGAGGCTTTTTGATCTTTTGGAGGAGAAACAGGTTTGGAAGCTTTCTCAAGAGCTTGTTTGGTGGGATGTTACTACTCAAACGATCGTAGAGCCTGAAGATATTGTTTGGAAAGAAGAGATGCAAACACAGCTTGAGGTTAAGTGTTTTGTGGAGACGAAGAATGATGTTTTTCCGCTCGTTATTGATGATCCTTTGCAGCTTTTTTCTGATAAAGGAGTACTTGTCCATCCGAATGATAAAAGATACAAAAAGCATATTGGTAAGAAAATTATTATTCCAATTCTTAATAAATCGATTCCTATTTTTTGA", "aa": "MSVYRKTQKKNQTLLSQFFGNEGIHFCSTFQNERIQRLFDLLEEKQVWKLSQELVWWDVTTQTIVEPEDIVWKEEMQTQLEVKCFVETKNDVFPLVIDDPLQLFSDKGVLVHPNDKRYKKHIGKKIIIPILNKSIPIF", "aa_hexdigest": "1c50d03ca371e2a5144a1f20412eaecc", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16436.881900000004, "isoelectric_point": 6.831614875793457 }, "id": "NKOCPLAKPK_192", "locus": "GCA003260355_00151" }, { "type": "cds", "sequence": "contig_1", "start": 77732, "stop": 77992, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAGGAAAACGCAACAGTATCGTCTTTCTTTGCAAGATGAAGTTATTGATGAAAAGGGATTCTTTGTTTCAGATATCCCAAACTTTGGAGGGAAGCCAGCTTTTGATTTTGTTGAAAATATTACTGAAACACTTGCTACGATAGGAAATCTTGTTTCTAAGCAGGAGGTCAAGAAAAAGATTCCTTATTCGAGTGAAACGAATACTCGTTTAATAAGGAGAATCGTTCCGGGTATTATTTTGGATTTTTCTCAGTATTGA", "aa": "MRKTQQYRLSLQDEVIDEKGFFVSDIPNFGGKPAFDFVENITETLATIGNLVSKQEVKKKIPYSSETNTRLIRRIVPGIILDFSQY", "aa_hexdigest": "bc87728f48577af00ef719024b330549", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9884.195999999993, "isoelectric_point": 7.985005760192872 }, "id": "NKOCPLAKPK_193", "locus": "GCA003260355_00152" }, { "type": "cds", "sequence": "contig_1", "start": 78011, "stop": 78178, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAGAAGGAATTTCCAGAGCTTTCGGAAGAAGGGCGTCAGCCATTTTCAGTGAAATTGTCTTTGGAATCCAAAAATATTTACCAGCCTAGATTGCCGTTTCTTTGTAATGTTATGACAGGAGAGGTGAAGTGTTTTAGGTATCAAATTTGTGAGAGGAGCTGTTGA", "aa": "MEKEFPELSEEGRQPFSVKLSLESKNIYQPRLPFLCNVMTGEVKCFRYQICERSC", "aa_hexdigest": "4e04035862a49d1a1691217b62560146", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6519.507299999999, "isoelectric_point": 6.196609687805175 }, "id": "NKOCPLAKPK_194", "locus": "GCA003260355_00153" }, { "type": "cds", "sequence": "contig_1", "start": 78200, "stop": 78469, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTATCTTCTTGTTGAGCAGCAACTGCCTTTTGAATTTTCAATTAGTGACCTTTTTGATGCGCTTTATTTTATGTCGGAAGAGAGGTGGAATGCTTTTTTTGATCAGTTTTCTTTTTCTGTTTCTAAGGAGGATTCTTTGTTTGCACTGATGAAATCTAATGATCATGAGATGATCTCTGAACGCATTGGATTAGAATTGGAAAAAATTCCATGGCTACAAATGGACGCAAAGGGGATTTTCCGCTTTCTGCCTACTGTATTATTCTGA", "aa": "MYLLVEQQLPFEFSISDLFDALYFMSEERWNAFFDQFSFSVSKEDSLFALMKSNDHEMISERIGLELEKIPWLQMDAKGIFRFLPTVLF", "aa_hexdigest": "ede8cff8df84ff272d37fe33f9508f29", "start_type": "TTG", "rbs_motif": "TTTTT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10646.136699999992, "isoelectric_point": 4.365371131896972 }, "id": "NKOCPLAKPK_195", "locus": "GCA003260355_00154" }, { "type": "cds", "sequence": "contig_1", "start": 78521, "stop": 78862, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGCTTGTTTCTCTAGGAGTCAGGGTTTGAAGCTTTTTTTTCAGCAGCAGCAAGTATTCCCTTTATTTTTGGAGTATTTTCTCTGGTTTAGTTTTGTGCTTCAGGAAACTTCAGGTTTTTCTTTTGTGCCTTTTCATGCTGATGAGAAAGCTTTCCCTTTACAGGCAAGGTGGGAAAGTGAGCTCTCTGATTATGGTTTAGATGTTCTAAGATTTACTGTTGCTCGTGGAAAAGCTTATACAGAGTCAGATTTGGCTGTTTCTTTTGAATATTTAAAGCATCTTTGGAATCTCTTTAAGGTGCTTTATGAGCAGGGCGTTTTTGAGTTGGATGAGGTTTGA", "aa": "MACFSRSQGLKLFFQQQQVFPLFLEYFLWFSFVLQETSGFSFVPFHADEKAFPLQARWESELSDYGLDVLRFTVARGKAYTESDLAVSFEYLKHLWNLFKVLYEQGVFELDEV", "aa_hexdigest": "b0a6786bca76b9333e65bf08f8c152e0", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13391.08219999999, "isoelectric_point": 4.765972328186035 }, "id": "NKOCPLAKPK_196", "locus": "GCA003260355_00155" }, { "type": "cds", "sequence": "contig_1", "start": 78977, "stop": 79675, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAAGACTTTACCAGAGGAGCATTTTCTTGGTATCTTGCCTTGGTAAAAGGGGAAGCTCATGAGGAATCTAGGTTCTTGGCAGGTCAGTTGTTTTTATCTCTTTTGGAGCTCTTATCTCCGATTATTCCTGAATTTATTTCGATGATTGAAGGAGTCTTGAAATTTCCTGTAAAGTCTGTGGTAAATCGAGAAGAACTTCATGGTGTGAAAGATTATAGGACCTTCCTTCTTTTTGATATATTGAAGGGAATTCAGCTCAAGAGAGCGGAGCTTCAGCTGAAAAAACATCTTCCTATTGCGTTGTGTGTTCAGGCGAATTCGGATATTTTACGTTTAATAGAATCTTATGAATCAAGCTTGAAATTGCTTTTTAAGACGGAAGAAATTCAGTATTTAGCTGCGAATGAGGTTTTCCCTTCAGGTTTTGAAACTTTTATGATTCTTGATATTACGGTTGGAATTAAAGCTCTTGATCTTCCTCAGCATGAGGATTGTTTGGCGCAGCTTGAGAAGGAGCATACTTCGAAATTGCAGACCTTGGAATATTTGAGGTCGACTTTAGTAGCGCTTTCTTTGAATCCTTTGGCGGATCCTGTAAAACTTGCTGAGAAGGAAAGTGAACTTGATCAACTTAAGTCTGAAATTCAGGAACTTGAGCTGAATATCAAAAAAATGAAGGCTACAAAGAAGGTGTAG", "aa": "MQDFTRGAFSWYLALVKGEAHEESRFLAGQLFLSLLELLSPIIPEFISMIEGVLKFPVKSVVNREELHGVKDYRTFLLFDILKGIQLKRAELQLKKHLPIALCVQANSDILRLIESYESSLKLLFKTEEIQYLAANEVFPSGFETFMILDITVGIKALDLPQHEDCLAQLEKEHTSKLQTLEYLRSTLVALSLNPLADPVKLAEKESELDQLKSEIQELELNIKKMKATKKV", "aa_hexdigest": "f6975efc895c1a638c4478e23c619d90", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 26473.659700000022, "isoelectric_point": 5.5123819351196275 }, "id": "NKOCPLAKPK_197", "locus": "GCA003260355_00156" }, { "type": "cds", "sequence": "contig_1", "start": 79724, "stop": 80140, "strand": "-", "frame": 3, "gene": null, "product": "Translation initiation factor IF- 2 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A948C4S3" ], "nt": "ATGAATGCGCTTCTCAAAAAGAAGGCTGAAGGGCTCAAAATTGAGATGAAAGTTTTTGATATTATTTATGAACTCACAGATTACCTCAATAATCTACTCCTCGGAATGGTAGAAGTAGAGAAAGAAGAAGTAATCGTCGGTAAGCTTGAAATCCTTGGTGTCTTCCACACAGAAACGAGAGAAATGACGGTTGGAGGAAGAGTAAAAGAAGGGAAGGTCAAGAATAAGCTTAAATTCAGAGTCTATAGAGGTGATGATATTTTGACAAACGGAGAAATCCTTTCTCTTCACCGTAATAAAGACGAAGTAAAAGAGGTAAATGCGGGTGAAGACTGCGGTATGAAAGTAAAAATCGGAAAGAGAATCGAAATGGGAGATATGCTTGAATTTTATGAAATGCAAGAAGTGAAAGCATAA", "aa": "MNALLKKKAEGLKIEMKVFDIIYELTDYLNNLLLGMVEVEKEEVIVGKLEILGVFHTETREMTVGGRVKEGKVKNKLKFRVYRGDDILTNGEILSLHRNKDEVKEVNAGEDCGMKVKIGKRIEMGDMLEFYEMQEVKA", "aa_hexdigest": "2931e28777b61b61eff579267a78e233", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A948C4S3", "query_cov": 0.9927536231884058, "subject_cov": 0.8106508875739645, "identity": 0.606, "score": 173.0, "evalue": 5.35e-53, "valid": false, "product": "Translation initiation factor IF- 2 domain-containing protein", "uniref50_id": "UniRef50_A0A948C4S3" }, "pscc": { "uniref50_id": "UniRef50_A0A948C4S3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A948C4S3" ], "product": "Translation initiation factor IF- 2 domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_198", "locus": "GCA003260355_00157" }, { "type": "cds", "sequence": "contig_1", "start": 80153, "stop": 80527, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF11987.13" ], "nt": "ATGGGAGGAGAACCTGTGCAAATCCTTGGATTTACTGAGCTTCCTGAGCCAGGAAGAATTGTTGAGGTGGTTTCCAACGAAAAAGAAGCTCATCAGAGAATTGCCTTTATCAAAGAGTCTGAAGCAAAAGCGAGTGGAGATGGAGCTTTACAACAATTCTTAGCTCAGTTAAAAGCTGGAGATCAATCAAAGATATCAGAATTAAGACTGATCCTCAAGGCTGATGGTTCCTCTAGTTTGGAGGCTCTCAAGCAGGCCGTTGATGGACTTTCGCTTCCAAAGAATGTGACAATCAAGGTGGTACATAGCGATGTAGGGTATTTCGGAGAAAGTGATCTTTCCTTAGCCCAAGCTTCAAAGGCATTATTGATCTGA", "aa": "MGGEPVQILGFTELPEPGRIVEVVSNEKEAHQRIAFIKESEAKASGDGALQQFLAQLKAGDQSKISELRLILKADGSSSLEALKQAVDGLSLPKNVTIKVVHSDVGYFGESDLSLAQASKALLI", "aa_hexdigest": "bc0ce0449b977788538e352eb8c5e7f6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF11987.13", "name": "IF-2", "length": 84, "aa_cov": 0.6774193548387096, "hmm_cov": 0.9523809523809523, "evalue": 1.241214562322521e-15, "score": 53.89198303222656, "start": 41, "stop": 124 } ], "seq_stats": { "molecular_weight": 13171.867499999986, "isoelectric_point": 5.154751014709471 }, "id": "NKOCPLAKPK_199", "locus": "GCA003260355_00158" }, { "type": "cds", "sequence": "contig_1", "start": 80546, "stop": 81577, "strand": "-", "frame": 3, "gene": null, "product": "Translation initiation factor IF-2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A930QQ02" ], "nt": "GTGTTCAAAAAAGAGGAGAAGCCTGCTCCAACACCAACAGCGAAGGTTCATAAAGAAGCTACAACTTCATCAAATCTCGTCAAGAAAACTGAGGTAACCATTGAAGAAAATATTACGGTAAAAGAATTTTCAGAAAAGATTGGAGTTCCACTTCCAGAAGTGATGAAAAAGCTTATTGCCAACGGAATTATGACAAGTTTGAATGCAAGTTTGGATTTTGATACAGCTTCTCTGATCGCTGAAGATTTAGGAGTTACTCTTAAGAAGAAAGAAGCAACGCTTGATGTACAGAGTTTTATGGAAGGAGATTTGCAGAAGATTTTGGATATTGATAAAGAGGCAGAAAAACAGGTTGAGAGAGCGCCAATCGTGACCGTGATGGGACATGTAGATCATGGAAAAACCTCGCTTTTGGACTACCTCAGAAAAACGAGCGTTGCAGGAGGTGAAGCCGGAGGGATTACGCAATCTATCGGTGCTTCTATGGTAAATTATAATGGAAAGCAGATTACTTTCATTGATACTCCAGGACATGAGCTTTTTACTTCACTCAGAGCTAGAGGTGCAAAGCTGACTAATATTGCGATTATCGTGATTGCAGCAGATGATAGTGTCATGCCACAGACTATTGAATCTATCAACCATGCTAAGTCCGCTGGCGTTCCAATTATTATTGCCGTAACCAAAATTGATAAGCCTGGAAATAATATGGATCAGATTAAAGCTGATGTTGCCAAATATGGACTGACTCCTGAAGATTGGGGAGGAGAAACGCCGTTCGTAGGAGTTTCATCCAAAACTGGTCAGGGTATTGATCTTTTGCTAGAATATGTACTTTTACAGGCTGAAATGCTTGAGCTCAAATACAATCCTGACAGACAAGCGGTAGGAGTAGTTGTAGATGCTTACAAAGATCCAAAACAAGGAGTGGTAGCAAGCTTGATCGTGCTGACTGGAACCTTGAAGAACGGAGATATTATCGTAGCTTACAATACTTATGGAAAAGTAAGGAGAATGCAGAACCGACTCTGA", "aa": "MFKKEEKPAPTPTAKVHKEATTSSNLVKKTEVTIEENITVKEFSEKIGVPLPEVMKKLIANGIMTSLNASLDFDTASLIAEDLGVTLKKKEATLDVQSFMEGDLQKILDIDKEAEKQVERAPIVTVMGHVDHGKTSLLDYLRKTSVAGGEAGGITQSIGASMVNYNGKQITFIDTPGHELFTSLRARGAKLTNIAIIVIAADDSVMPQTIESINHAKSAGVPIIIAVTKIDKPGNNMDQIKADVAKYGLTPEDWGGETPFVGVSSKTGQGIDLLLEYVLLQAEMLELKYNPDRQAVGVVVDAYKDPKQGVVASLIVLTGTLKNGDIIVAYNTYGKVRRMQNRL", "aa_hexdigest": "df9f819067fdb193c38ab1c8a11014bd", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0009FAFE70", "query_cov": 0.8104956268221575, "subject_cov": 0.8475609756097561, "identity": 0.529, "score": 281.0, "evalue": 8.25e-90, "valid": false, "gene": "infB", "product": "translation initiation factor IF-2", "uniref50_id": "UniRef50_A0A930QQ02", "kegg_orthology_id": "K02519", "go_ids": [ "GO:0003743", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0006413" ] }, "pscc": { "uniref50_id": "UniRef50_A0A930QQ02", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A930QQ02" ], "product": "Translation initiation factor IF-2" }, "genes": [], "id": "NKOCPLAKPK_200", "locus": "GCA003260355_00159" }, { "type": "cds", "sequence": "contig_1", "start": 81650, "stop": 82189, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCTCGTATTGATGATGTAAAGAAAGATTTAAACCTAGATGAAGCCACGTTTGCCAAAGTATACGAAAAAGTTATGGGCAAAAAATTGACCGCAAAAACGGCAACAATTTCTGATAGCAATCTAGAAAAAATGAAATATATCATAGATCAGCTTCCAAAGAAAGCTGCTAAAAAAGCCGCTCCTGCCAAAAAAGCTGATGATGGGAAAATTTTGAAATCAGACGAAATAGGATTTGGAGGAGGCTTCCTTTCTGGACTAGGATTTACCAAACAAGCAGAAAAACCTGAAAGAGAAGATGAAGATAGCTTAGAGCTTCATAATATCGAAATCCCGCTTACTTCAAATAAAGAGACAAGTCAAGAAGAGCTTTCTTTCTCAGATGCACCAAAAATAAGAAACGCTAGGGTTATTGCAAGGGCAGAACCTCGTCCAGTAGAATCTTCTAAAAAACCAAAATCAACTGCGTCAAAGGCAGGAGGAAATATCTCTATCGAACAGGGGACTTACAAAGCCAAAGAAAAACCACAAAAAAGCTGA", "aa": "MPRIDDVKKDLNLDEATFAKVYEKVMGKKLTAKTATISDSNLEKMKYIIDQLPKKAAKKAAPAKKADDGKILKSDEIGFGGGFLSGLGFTKQAEKPEREDEDSLELHNIEIPLTSNKETSQEELSFSDAPKIRNARVIARAEPRPVESSKKPKSTASKAGGNISIEQGTYKAKEKPQKS", "aa_hexdigest": "639a68eaffdebf3a50a0fe322ee06f4b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19635.141, "isoelectric_point": 9.267088508605958 }, "id": "NKOCPLAKPK_201", "locus": "GCA003260355_00160" }, { "type": "cds", "sequence": "contig_1", "start": 82379, "stop": 82789, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCTCTCAGAGCTCAGAGGTAGAGAATTTATGCTCGAAGCGGGCTATGAGTGGAGCGATGAGAGGTTTAATGAAAATCCCAACACAAAGGAATATCCCAAAAAAGCTCAGGAGTATGATCATATTAGGAAGATACACTTCTCGAAGGATACCTCATACGGCTTCTCTCATCGCGATGATTGTGTAGGTGAAAGGGAGAAATGGATTGATTGCTTGAAAAAAGCTACCAGAAAGTTCTATCGGGAAGGTGCCGCCAGCTCAGGAAAGTTGGAGCACCAAAAAGACGATGACGATTGCTTTCCCTGCACTTCAGAATGTGGCGACGGTGGTATAGATAATCATCGAGAAAACCATTGCTGCTCGGAGCGTGATTCCTATGAATGCTCCTTTATTAGCGACATAAGCGTTTAA", "aa": "MLSELRGREFMLEAGYEWSDERFNENPNTKEYPKKAQEYDHIRKIHFSKDTSYGFSHRDDCVGEREKWIDCLKKATRKFYREGAASSGKLEHQKDDDDCFPCTSECGDGGIDNHRENHCCSERDSYECSFISDISV", "aa_hexdigest": "228c984ac572e81dc1896b720a304320", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15925.24609999999, "isoelectric_point": 5.141962242126463 }, "id": "NKOCPLAKPK_202", "locus": "GCA003260355_00161" }, { "type": "cds", "sequence": "contig_1", "start": 82688, "stop": 82981, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCGCCTTTCTTTACGACCTTAGCCATCCGAGTAGGCTCTCTGCTCAATATCTCACTCTTTACTACCAAAATCAGAGAGAAAACCTTCTACCACTGCAAACACTATCAAAAATACCTAGGGAAATGGCTCCTCTTCCTCAGCATAGCACTCATTCAGGGAATAATTGTTGCTTTGGGAGATATGTTTTTATTAAACGCTTATGTCGCTAATAAAGGAGCATTCATAGGAATCACGCTCCGAGCAGCAATGGTTTTCTCGATGATTATCTATACCACCGTCGCCACATTCTGA", "aa": "MSPFFTTLAIRVGSLLNISLFTTKIREKTFYHCKHYQKYLGKWLLFLSIALIQGIIVALGDMFLLNAYVANKGAFIGITLRAAMVFSMIIYTTVATF", "aa_hexdigest": "7681f059efc3dade49c1f37fcb8688d5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10963.102499999988, "isoelectric_point": 9.933562660217284 }, "id": "NKOCPLAKPK_203", "locus": "GCA003260355_00162" }, { "type": "cds", "sequence": "contig_1", "start": 83102, "stop": 83572, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTGATCACCTCCTTCCTCATACGCCTTTGACCGCCCCATTCAATAAACTACTTCAAAAGCTCAATCTCCTCCGTCAAAAAAGCATTTCCATCCGTTCGACTCTTTCACCACTCTCCCAAGACGCCAAAAAAGTCATCGACTTTAGCAAGGATACCCAAACAGCGATCCAAGGCATTCGTCAGGACATCCGCACAAGCCTTGATGATCTCCATGACGATTATGAAACAAATATTGAACCTGCACTGCGTCAAGTCCTGAATCACACCCAAGATCTTTCAACCAGCGGAATAGAAAAACTCGACCGCCTCGAGAATAAACTTCCTGATCTCCAGAGAAACCTTGAAGAAGGTGCAAGGATCATCAATACAGAAACAGAAAAAGTCAGATCCTTCCAAGAGCAACTCCCCAAACTTCAGAAGGATGTCGGCACGATTGATCAGCACCTCCAAAAACTGAAGGCTAGCTGA", "aa": "MIDHLLPHTPLTAPFNKLLQKLNLLRQKSISIRSTLSPLSQDAKKVIDFSKDTQTAIQGIRQDIRTSLDDLHDDYETNIEPALRQVLNHTQDLSTSGIEKLDRLENKLPDLQRNLEEGARIINTETEKVRSFQEQLPKLQKDVGTIDQHLQKLKAS", "aa_hexdigest": "4396bb1fad86bc322b9f4e94a1ffe1b8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17853.063899999986, "isoelectric_point": 6.516385841369629 }, "id": "NKOCPLAKPK_204", "locus": "GCA003260355_00163" }, { "type": "cds", "sequence": "contig_1", "start": 83606, "stop": 84559, "strand": "-", "frame": 3, "gene": null, "product": "ABC-2 type transporter transmembrane domain-containing protein", "db_xrefs": [ "KEGG:K01421", "SO:0001217", "UniRef:UniRef50_A0A328EFU0", "UniRef:UniRef90_A0A328EFU0" ], "nt": "ATGAAACGAATCCGACGTATCTATACAGCTGATCTTAAACGTATCTTTTCCAATCGAGGTGCAATGGTCATTGTGCTAGGACTCTGCATTTTGCCGTCGCTCTATGCCTGGTTTTACCTAAAATCCTCCCGAGACCCTTATGGGAATACCAGAGGCATCAAGGTCGCCGTCGTCAATAATGATATTGGAGCTGTTTTTAAGGAGGAATTTATCAATATTGGAGCAGAAGTTATCAAAGAGCTAAAGACAGATCAAAATATCGGTTGGGTTTTTGTCGATGAAAATATCGCGAACCAAGGGACAAAGCTAGGAAAATATTATGCTTCTATTGTGATTGAAAGCGGATTTAGCGAAAAGATGATCACACTTCTTGATGAGTTTCCTCAGCACCCAGAAATCCGTTATACCGTCAACGAGAAGCTCAACGCCATAGCCCCAAAAATCACCAGCAAAGGAGCAACGACGATCAAAGAAAATATCCAAAAATCTTTCATAGACACCGTCAATCGCGTCCTGATGGAAAAATTGAATCTCGTGGGATTCGATCTCCAAGCTAGCAAAACAAGCGTATATGGTTTGATCGACTTCGTGCATGATGCTCGTGAAAGTTTAGATGCCGTTGATGAAAAAATCAATAAAATTCTGGAGATTTCCTACCGTTCGAGATACCAACTCGAGGAAGCCTATGGCAAACTCCCAACCCTCAAAAACGACATCAATCAAGGGAAAGAAGCGCTCTACCAAAGCAGGATACTTGCCGAGCAAAGCCTCAAACTTTTGGATACCGTCCCAGATCAAATCCAAACCCACAAAACCTCAATCCAAGACATCGCTAGAAGCATTGATAGCGAATTTTCTGCTATCCTTAGCTATGCCGATCAGAAAAACCAGAACCTCGAGAACGATCTTAACCAGATCACCACAAGACTCACCAACCTCGAAAATGAGCTTTGA", "aa": "MKRIRRIYTADLKRIFSNRGAMVIVLGLCILPSLYAWFYLKSSRDPYGNTRGIKVAVVNNDIGAVFKEEFINIGAEVIKELKTDQNIGWVFVDENIANQGTKLGKYYASIVIESGFSEKMITLLDEFPQHPEIRYTVNEKLNAIAPKITSKGATTIKENIQKSFIDTVNRVLMEKLNLVGFDLQASKTSVYGLIDFVHDARESLDAVDEKINKILEISYRSRYQLEEAYGKLPTLKNDINQGKEALYQSRILAEQSLKLLDTVPDQIQTHKTSIQDIARSIDSEFSAILSYADQKNQNLENDLNQITTRLTNLENEL", "aa_hexdigest": "29e5b1f7cbcc066dc8a2d1140c43e38c", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 83303, "stop": 84559, "observations": { "mutation": [ 83608 ], "stop": [ 83608 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EFU0", "query_cov": 1.0, "subject_cov": 0.4360385144429161, "identity": 0.978, "score": 549.0, "evalue": 5.6e-190, "gene_start": 1, "gene_end": 317, "reference_start": 1, "reference_end": 317, "reference_sequence": "MKRIRRIYTADLKRIFSNRGAMVIVLGLCILPSLYAWFYLKSSRDPYGNTRGIKVAVVNNDIGAVFKEEFINIGAEVIKELKTDQNIGWVFVDENIANQGTKLGKYYASIVIESGFSEKMITLLDEFPQHPEIRYTVNEKLNAIAPKITSKGATTIKENIQKSFIDTVNRILMEKLNLVGFDLQASKTSVYGLIDFVHDARESLDAIDEKINKILEISYRSRYQLEEAYGKLPTLKNDINQGKEALYQSRTLAEQSLKLLDTVPDQIQTHKTSIQDIARSIDSEFSAILSYADQKNQHLENDLNQITTRLTNLENELGRQAQKITQRRDMIDHLLPHTPLNAPFNKLLQKLNLLRQKSISIRSTLSPLSQNAQKVIDFSKDTQTAIQGIRQDLRTSLDDLHDDYETNIEPALRQVLNHTQDLSTSGIEKLDRLENKLPDLQRNLEEGARIINTETEKVRSFQEKLPKLQKDVKTIDQHLQKLKASGKLENFIEVATLDPNRFSEFIAEPVKLVENKLFSIPNYGSAMSPFFTTLAIRVGSLLNISLFTTKIREKTFYHCKHYQKYLGKWLLFLSIALIQGIIVALGDMFLLNAYVANKGAFIGITLRAAMVFSMIIYTTVATFGNAGKAIVIVFLVLQLSGAGGTFPIELSGSFFQAINPFLPFTYTIIAMREAVGGILREVYLPNMIILLSFFGIFLCVGIFIKPLIAPLIARFEHKFSTSELGEH" }, "paralog": false, "identity": 0.9712918660287081, "score": 741.0, "evalue": 1.08e-267, "description": "Internal stop codon at 83608. Nonsense mutation around 83608.", "psc": { "uniref90_id": "UniRef90_A0A328EFU0", "product": "ABC-2 type transporter transmembrane domain-containing protein", "uniref50_id": "UniRef50_A0A328EFU0", "kegg_orthology_id": "K01421", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EFU0", "UniRef:UniRef50_A0A328EFU0", "KEGG:K01421" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EFU0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EFU0" ], "product": "ABC-2 type transporter transmembrane domain-containing protein" } }, "id": "NKOCPLAKPK_205", "locus": "GCA003260355_00164" }, { "type": "cds", "sequence": "contig_1", "start": 84705, "stop": 84926, "strand": "+", "frame": 3, "gene": null, "product": "HEPN domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MZH9", "UniRef:UniRef90_A0A660MZH9" ], "nt": "ATGCTTGATGTGAAAAAAGAGGCAGCAACGTTTAGTCGTTTGTCGCACGATGAAAAGCTTGAACGTGTCCAAAAATGGCTCGGAGAGCTGGCGTATCATTATGACTATTTTCGTGATTTGGACAGCTATGTGCATATCCATAAAGCATCACTTGATGATGTGTTTTTGGAGGCGATGTTTCAGATTATCCTCAATTTAGCCAAGGAAGTGGAAAAGCTTTAA", "aa": "MLDVKKEAATFSRLSHDEKLERVQKWLGELAYHYDYFRDLDSYVHIHKASLDDVFLEAMFQIILNLAKEVEKL", "aa_hexdigest": "d48ff5b416f89e835547b66c9fbc26c0", "start_type": "ATG", "rbs_motif": "TTATT", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 84613, "stop": 84926, "observations": { "deletion": [ 84640 ], "stop": [ 84673 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A660MZH9", "query_cov": 1.0, "subject_cov": 0.7019230769230769, "identity": 0.973, "score": 141.0, "evalue": 2.21e-42, "gene_start": 1, "gene_end": 73, "reference_start": 32, "reference_end": 104, "reference_sequence": "MSTRKKSLIFFDFCKVLKYTGGDFFIFSLLEMLDVKKEAATFSRLSHDEKLERVQKWLGELAYHYDYFRDLDSYVHTHKASLDDAFLEAMFQIILNLAKEVEKL" }, "paralog": false, "identity": 0.9615384615384616, "score": 196.0, "evalue": 2.22e-66, "description": "Internal stop codon at 84673. Frameshift due to deletion around 84640.", "psc": { "uniref90_id": "UniRef90_A0A660MZH9", "product": "HEPN domain-containing protein", "uniref50_id": "UniRef50_A0A660MZH9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A660MZH9", "UniRef:UniRef50_A0A660MZH9" ] }, "pscc": { "uniref50_id": "UniRef50_A0A660MZH9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MZH9" ], "product": "HEPN domain-containing protein" } }, "id": "NKOCPLAKPK_206", "locus": "GCA003260355_00165" }, { "type": "cds", "sequence": "contig_1", "start": 84945, "stop": 85310, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGAAGTATGGAAATTATTAAAAATGATGAAGCGGCAGATAAAAATCTAAAGTCTGAACTGGATCAGTTGGTCGGTGGGATCAATATCAAAGAAAAAACTGAAAGCTTTGGTGCGATCGATCAGTTTTTTGAAAAGTTGAATAGTTTGCCAGAAGAACAGAAAAATTTAGCTCTTCAGCATGCGCTCGGTAAGAGCAGTCTTACGATTGATGGCTCCAAGAAGAGACTTAGCTCGTTGGCGAAACTTTTTGATAAGGCGGTCGATCAGGAGAATGGAACATGGAAAATTAAGCTGAAAGAGGGTGTTTCTTTGCGTGAGTCGTCCTCGTCTGATGTAGTGAATCCTGAGACAAGCAATTCTTGA", "aa": "MRSMEIIKNDEAADKNLKSELDQLVGGINIKEKTESFGAIDQFFEKLNSLPEEQKNLALQHALGKSSLTIDGSKKRLSSLAKLFDKAVDQENGTWKIKLKEGVSLRESSSSDVVNPETSNS", "aa_hexdigest": "12d37e3150c5c6ad4b099a86b0e74013", "start_type": "ATG", "rbs_motif": "TTTTTA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13367.879099999984, "isoelectric_point": 5.496069145202636 }, "id": "NKOCPLAKPK_207", "locus": "GCA003260355_00166" }, { "type": "cds", "sequence": "contig_1", "start": 85398, "stop": 85667, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAAGCAAAACTCCTGCTGGAGCAGGTGAAAAAAAAGATGAAGCTGTTCAGACGACTCCAGCAACGGAAGAAGAACCGGATGATGCTGATCTTGAGGGGCTTAGTGCTGAAGAGCAGGAACTTGTTTCTTCGCTACTGAATGAAAAGAACTCACCTTTAGCGTATTTGATCCAGAAGAGCTCGAATTTTGCCAAGATTCAGGCTCAGAAAGGATATGGAGCGGATGCGGATACTTTGCTAGAAATTCGTGAAGATAAGATTCTCTGA", "aa": "MASKTPAGAGEKKDEAVQTTPATEEEPDDADLEGLSAEEQELVSSLLNEKNSPLAYLIQKSSNFAKIQAQKGYGADADTLLEIREDKIL", "aa_hexdigest": "45ffc90f362420fb83c9219ff2268e0e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9566.41029999999, "isoelectric_point": 4.2714731216430675 }, "id": "NKOCPLAKPK_208", "locus": "GCA003260355_00167" }, { "type": "cds", "sequence": "contig_1", "start": 85758, "stop": 86108, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTGTCGATCGGGTTCATTTCTCAAGATATGAAAGAAAGAATTGAGGCGAGCGGACCCGAGAGCGTGAAGTCGCTCTGGAATGATGAAGCGTATGAGCGTAAAAGTCCTTATACCAATAATTTTGCTTTAAAAAAGAAAGAAAATGCTGATTCTGATCAGCAGGTAGTTGATGGGAAAACAGATGTTGTAGATGAAGCTTTGCAGCAAACTCAACCAGGAGTAAAGAATGAGGATGGAGAGAAATCTGCTGAATCAACGAATGAGCTTAAGCGATGGCTTGTGGATGGTATCCCTCATGAGATTGTTAAGGTTTCTTCTTTTGATCAGCTTCCAAAGGATGAAAATTGA", "aa": "MLSIGFISQDMKERIEASGPESVKSLWNDEAYERKSPYTNNFALKKKENADSDQQVVDGKTDVVDEALQQTQPGVKNEDGEKSAESTNELKRWLVDGIPHEIVKVSSFDQLPKDEN", "aa_hexdigest": "521d5472aad4d210b5bdedc15a791bdb", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13071.212199999993, "isoelectric_point": 4.586986351013183 }, "id": "NKOCPLAKPK_209", "locus": "GCA003260355_00168" }, { "type": "cds", "sequence": "contig_1", "start": 86148, "stop": 86405, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGCGGTGGCAGGCAAGATCTTTCATTGTTTTGATAATGGGAAATGTCAAGATATGAAAACGGAAGAAATGGCTGATACGAAAGATGTTGTAGCTAAGGTAGCGAAACTTCAGGAACAACTTCCTACGAAGAAAGCGAATTTAATCAAAGCGTTTGAGCTTCAAGAAATGGATGGAGGCTATTATACCAGAGAAGGAGACGCTTCTCTTTATACGATTGATGAGGTGGGGACGCTCTATGCTGCTGGACCGCACTGA", "aa": "MAVAGKIFHCFDNGKCQDMKTEEMADTKDVVAKVAKLQEQLPTKKANLIKAFELQEMDGGYYTREGDASLYTIDEVGTLYAAGPH", "aa_hexdigest": "ac415429e78a65093f09b71ccae30c96", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9389.588699999995, "isoelectric_point": 5.01708698272705 }, "id": "NKOCPLAKPK_210", "locus": "GCA003260355_00169" }, { "type": "cds", "sequence": "contig_1", "start": 86616, "stop": 86870, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTTTTGAGGGATTAGAGACGCTAGGGCATTCGGTTCAAGAACCTGCTAAAGATGTGGTAAAAGATTCTTTGCAAATGCTCTATACCCCTCTTTGGGTCCCTCAGCCTGTTGAAAATCAAGTTCAAATTCCTTTTAAACCGCAAGTGACGACTCCTCAAGAGCCTTGGGCTGCTCGGGATTCGGCCGATTTAGAAAAGCCTTGGAAGGAGGTTGCAGAACTGCCTGAAGAGGTGAAGTATATTGAAGTGTAG", "aa": "MVFEGLETLGHSVQEPAKDVVKDSLQMLYTPLWVPQPVENQVQIPFKPQVTTPQEPWAARDSADLEKPWKEVAELPEEVKYIEV", "aa_hexdigest": "61d9901183059fdf756b55c96757cdb1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9628.851400000003, "isoelectric_point": 4.417151451110841 }, "id": "NKOCPLAKPK_211", "locus": "GCA003260355_00170" }, { "type": "cds", "sequence": "contig_1", "start": 87091, "stop": 87447, "strand": "-", "frame": 1, "gene": null, "product": "Pseudouridine synthase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3D0USY2", "UniRef:UniRef90_A0A328EJ58" ], "nt": "GTGAAAAAATCTGACTTTAAGCTTTTTGGTGGAGAAATGCTGACAGTTTTTGGTCAAGAAATCAAGGTGCTTGAAAAAGTTTATGCGATTCTCTACAAGTCAGCATGCTATATTTCCTCAGATGAAGATGAATTTGGCTATCCGAGCTACAAAAAGCAGATGCTTGATTGCCCCTATGTGCATTTACTGCATGTTGCAGGAAGACTTGATGTCGATACAGAGGGTCTCCTCTTGCTTTCCAATGATGGGCAGTTTATTCATCAGGTCATCAGCCCAAAACGAGATAAAGAAAAAGAATACGAAGTCTGGCTCCAATCACCGATCAACCTCACAGACTGCGAACAGCTCCAAAATTGA", "aa": "MKKSDFKLFGGEMLTVFGQEIKVLEKVYAILYKSACYISSDEDEFGYPSYKKQMLDCPYVHLLHVAGRLDVDTEGLLLLSNDGQFIHQVISPKRDKEKEYEVWLQSPINLTDCEQLQN", "aa_hexdigest": "6e2f487965762388a0051bce25b28803", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 86875, "stop": 87549, "observations": { "mutation": [ 86931, 87093, 87474 ], "stop": [ 86931, 87093, 87474 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EJ58", "query_cov": 1.0, "subject_cov": 0.5267857142857143, "identity": 0.992, "score": 241.0, "evalue": 1.68e-79, "gene_start": 1, "gene_end": 118, "reference_start": 35, "reference_end": 152, "reference_sequence": "MQRLDKYLANLGLISRREATNACKSGSILVNGESVKKSDFKLFGGEMLTVFGQEIKVLEKVYAILYKSACYISSDEDEFGYPSYKKQMLDCPYVHLLHVAGRLDVDTEGLLLLSNDGQFIHQVISPKRDKEKEYEVWLQSPINLTDCEQLQNGVQLDDGYLTKPAKVQQLEEKKILLTITEGKYHQVKRMLEAVRNKVIYLKRLRIGSRTLEGLEKGERKLIET" }, "paralog": false, "identity": 0.9866071428571429, "score": 434.0, "evalue": 8.13e-158, "description": "Internal stop codon at 86931, 87093, 87474. Nonsense mutation around 86931, 87093, 87474.", "psc": { "uniref90_id": "UniRef90_A0A328EJ58", "product": "Pseudouridine synthase", "uniref50_id": "UniRef50_A0A3D0USY2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EJ58", "UniRef:UniRef50_A0A3D0USY2" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3D0USY2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3D0USY2" ], "product": "Pseudouridine synthase" } }, "id": "NKOCPLAKPK_212", "locus": "GCA003260355_00171" }, { "type": "cds", "sequence": "contig_1", "start": 87579, "stop": 88115, "strand": "-", "frame": 2, "gene": null, "product": "Elongation factor 4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7W4E3G8", "UniRef:UniRef90_A0A7W4E3G8" ], "nt": "ATGGCACTTTGTCAGGAATATAGAGGGAAACTCCAGCATATGGAATACTTGGATGCAACGAGAGTCGTTTGGCATTACGAGCTTCCTATGGGCGAAATCATCGTTGATTTTTATGATCGCTTGAAATCTGCGACGAAAGGCTACGCAACGATGAATTATGAATTTAAAGGTTATCATGCAGATGACCTCGTGAAACTTGACATTTTTATCAATAATGAGAAAGTGGAGGCTTTAACCTGGGTTGTGCATAATGATAAAGCCTATTATCTCGGTCGTGAAGCAGTAGAAAAGTTAAAAACGCTGATCCCAAAACATCTTTTCGCGATTCCTCTTCAAGCCGGACTTGGAAATAAAATCATCGCTCGTGAAAACATTTCTGCTATCAAAAAAGACGTCCTTGCTAAATGTTATGGTGGAGATGTGAGTAGAAAAAGAAAGCTCCTCCAAAAGCAAAAAGAAGGAAAGAAAAGAATGAAAGCTATCGGAAATGTGGAAATCCCGACTGATACGTTCGTAAAGATGATCACGAGAGACTAA", "aa": "MALCQEYRGKLQHMEYLDATRVVWHYELPMGEIIVDFYDRLKSATKGYATMNYEFKGYHADDLVKLDIFINNEKVEALTWVVHNDKAYYLGREAVEKLKTLIPKHLFAIPLQAGLGNKIIARENISAIKKDVLAKCYGGDVSRKRKLLQKQKEGKKRMKAIGNVEIPTDTFVKMITRD", "aa_hexdigest": "0f0da1f0ec0b003e7e463b95db7a6b72", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A7W4E3G8", "query_cov": 1.0, "subject_cov": 0.8811881188118812, "identity": 0.9440000000000001, "score": 340.0, "evalue": 9.37e-118, "valid": true, "product": "Elongation factor 4", "uniref50_id": "UniRef50_A0A7W4E3G8" }, "pscc": { "uniref50_id": "UniRef50_A0A7W4E3G8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7W4E3G8" ], "product": "Elongation factor 4" }, "genes": [], "id": "NKOCPLAKPK_213", "locus": "GCA003260355_00172" }, { "type": "cds", "sequence": "contig_1", "start": 88158, "stop": 88397, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATATCAAAAGTCTGATTCAGACTGGGCTTTATCGTGAAATTCTCAAAAAAGAAAATCTGACGCTATCCGCACAAGAACAGGAACATTTAGAACACTCCGAAGGGATTTTCGGAACCATGCTAGAGGAGCAGACTTTTGTGAAACTTGCTGAACTCTTGAAACCACGAATCGTCGTAAAATCAGGTTCCGATATGATAGATCAAGGAATGATTGAGGAAATCTTAGAGCCGATCTGA", "aa": "MNIKSLIQTGLYREILKKENLTLSAQEQEHLEHSEGIFGTMLEEQTFVKLAELLKPRIVVKSGSDMIDQGMIEEILEPI", "aa_hexdigest": "7244c68eb27d28a23f3317defbf95863", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9026.387699999997, "isoelectric_point": 4.789901542663575 }, "id": "NKOCPLAKPK_214", "locus": "GCA003260355_00173" }, { "type": "cds", "sequence": "contig_1", "start": 88398, "stop": 88526, "strand": "-", "frame": 2, "gene": null, "product": "Elongation factor 4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1V5ZNK7" ], "nt": "ATGCTCCATATGGATATCATCAAGGAAAGGCTTTTTCGTGAGTATGGTCTGGATACGATTTTCACGATTCCAACCGTAATGTATCTTGTAAAGTCCAAAAACCTTTCCATTCCGCTGATCAAAACTTGA", "aa": "MLHMDIIKERLFREYGLDTIFTIPTVMYLVKSKNLSIPLIKT", "aa_hexdigest": "fe082fee0b40957e8acdbe1adfe98553", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A1V5ZNK7", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.69, "score": 57.0, "evalue": 4.23e-10, "valid": false, "product": "Elongation factor 4", "uniref50_id": "UniRef50_A0A1V5ZNK7" }, "pscc": { "uniref50_id": "UniRef50_A0A1V5ZNK7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1V5ZNK7" ], "product": "Elongation factor 4" }, "genes": [], "id": "NKOCPLAKPK_215", "locus": "GCA003260355_00174" }, { "type": "cds", "sequence": "contig_1", "start": 88536, "stop": 88808, "strand": "-", "frame": 2, "gene": null, "product": "Elongation factor EFG domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3D0UVD8" ], "nt": "GTGACCGGACAAAAATCGGTAAGAGATGTGAAAATTGGAGATACTATCATCAAAACCAAGATCAATAAAAACGAAGGAACCAGCAAATATCATCAGTATTTGATTCCTGGTTTTAGAAAAGTCACTCCTTATGTGTATGCCGGGGTTTATCCGATTGACTCTACTGAATACGACAAACTCAAAGATAGCCTCGAGAAGCTTTCGCTCAATGATAGTGCAGTTGTCTATGAAATGGAGGATAGTAAGGCACTTGGACTTGGGTTTCGTTGCTGA", "aa": "MTGQKSVRDVKIGDTIIKTKINKNEGTSKYHQYLIPGFRKVTPYVYAGVYPIDSTEYDKLKDSLEKLSLNDSAVVYEMEDSKALGLGFRC", "aa_hexdigest": "c2704af945aa4bc7e64ededef872040f", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3D0UVD8", "query_cov": 0.9444444444444444, "subject_cov": 1.0119047619047619, "identity": 0.741, "score": 117.0, "evalue": 9.92e-33, "valid": false, "product": "Elongation factor EFG domain-containing protein", "uniref50_id": "UniRef50_A0A3D0UVD8" }, "pscc": { "uniref50_id": "UniRef50_A0A3D0UVD8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3D0UVD8" ], "product": "Elongation factor EFG domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_216", "locus": "GCA003260355_00175" }, { "type": "cds", "sequence": "contig_1", "start": 88869, "stop": 89024, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCTGAAATTTCTAGAGCATTGATTTTTGATTCGGTCTTTGATCCCTATAAAGGAGTTCTCGCCTATGTAAAAGTCGTAGATGGCTCATTTTCTGCAGGAGACGAGGTCTATTTGATCCACTCAGAAAAGAGCTTCCAACCAACAGAAGTCTGA", "aa": "MSEISRALIFDSVFDPYKGVLAYVKVVDGSFSAGDEVYLIHSEKSFQPTEV", "aa_hexdigest": "11a5dfa08b45bed8aec87e7e3685f07a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5672.3284, "isoelectric_point": 4.499567985534668 }, "id": "NKOCPLAKPK_217", "locus": "GCA003260355_00176" }, { "type": "cds", "sequence": "contig_1", "start": 89142, "stop": 90707, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAAGTTTTATTGTTATAAAAAATCAGATTTTATTATTTTTTATGGAAAATTCGGTTATAATTGAGTTAAGTTTTTTATTCATCAGTGTGATCGAGATGGGGAAAAAGAAGAAACAGCATCAAACGAATTCTTCCCTGTACTCTTCTGCTTTTAGTATTGAGCAGCAGAGGGCTCTTTCTGTAAATAAGGCGATCTCTGGTCATGTTGATGAGCTTGCTCAAACGCTTTCTTATGCTCCAAACTTTGATATTGATTCTACCTATTTAGACCTGGAAGCAAGGTTTGATTTGTCAGCTGGGAAGGGAATCCTTCGCGATATGATCCAAAATAGGAAATCTGTTGACTATGGGACGGTGAATTTAGAAAATTTTTCGCTAAAAGAGATCGAGTCTTTGAAGTTTTGGGAAATCCCTACGTCGAATCCACTTTTTGTTGCTAAGCTTGAAGAAGCAGTGATTCAGTGCATTATGGATGAGTTTGCGGCTCAACTCAATCCCGCAGAACAACGTAGTTTTACGGAGGCTGTTCGCTATCAGAGCAAGAGTTTGCCTTCTTTTCTCAGTGCGGTTCATGCGTATGTTGATTCTCTCAATAACTTTCAGCAAAAATCTCGTTTCCAAAAAATGATTCAGACTTTTGTGCTTCCTTTGAGAAAAGTTGTAGATCAGCTCGAGGCTTCTGGTGAAGATGTGACTGCAATCAAGACAAAAGCATTTCAAGATCGAGCACATGACCAACAAACTTTTGTAGATGCTTTTGTGAGTAAGGCGGTGATTTCTAGGCTTCACAAAAGAGTTGAAGAGATGAAAATTCTGATCAATGATGTGAAAGATACTTTTGATAAGGTTGTCCCATTCTTTCCTACGATTTTTCAGCAATTCCCTGCGAACTTTTCTGCTCTTTTGCAGGTGTTTCCGGAGAAAAAACAAGTCTGTGATGCACTTGAGAAAGAATATGAAGCAGCGACTTCTGATGCGGAGCGTGGTCCTGTAGCAGAGAAGATAAATGCTCTTTACTGGGAAGTCTATCTTGCAGATTTAACTACTAAGGAGCCAAAACTTTGTGCCTATCTGACTGAACTGAAAAAATCAGATTTTGACTTTTCTCTCTTATCTGATCAGAGCGGATTTTTCTCTCTGATTGCTCAGATGAGGCTTAAGCAGATGAAAGATGGCACGATGCTATCGTTTTTTGGTCAGGATACTGCTGCTTTTGAGAAGTTTTTTTTGGAACTTTTTGATCTCAATACGTCTGAAATTTCGGTTAGTGGCTATACGATTCCTGTAAAAAAGAAGGTACTTCCGGGTAAGCATTGGGATTATAATAATTTTTTTTATGGACAGTCCTCTGATGCGCTTCCTCTAGAGTATGAGCTTGATATTAGTAAAGTTCCCCTTTCTGTAGAGGATAGATATTCTTTTGAGAGGCTTTTTGCTTATGGGAAAAATCCGGCTTCTCCGGGGATTTATTCCCTCAAAGGTAATGATCTTGGAAAACTCTTGTTCCTTTTTAAACTTGGGCAGGACAATTCTATCTTTGACAATTATGATCCGAGAGCTTGA", "aa": "MASFIVIKNQILLFFMENSVIIELSFLFISVIEMGKKKKQHQTNSSLYSSAFSIEQQRALSVNKAISGHVDELAQTLSYAPNFDIDSTYLDLEARFDLSAGKGILRDMIQNRKSVDYGTVNLENFSLKEIESLKFWEIPTSNPLFVAKLEEAVIQCIMDEFAAQLNPAEQRSFTEAVRYQSKSLPSFLSAVHAYVDSLNNFQQKSRFQKMIQTFVLPLRKVVDQLEASGEDVTAIKTKAFQDRAHDQQTFVDAFVSKAVISRLHKRVEEMKILINDVKDTFDKVVPFFPTIFQQFPANFSALLQVFPEKKQVCDALEKEYEAATSDAERGPVAEKINALYWEVYLADLTTKEPKLCAYLTELKKSDFDFSLLSDQSGFFSLIAQMRLKQMKDGTMLSFFGQDTAAFEKFFLELFDLNTSEISVSGYTIPVKKKVLPGKHWDYNNFFYGQSSDALPLEYELDISKVPLSVEDRYSFERLFAYGKNPASPGIYSLKGNDLGKLLFLFKLGQDNSIFDNYDPRA", "aa_hexdigest": "7309c7e0416bb465c61064fa618b571b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 59410.099500000164, "isoelectric_point": 5.306908988952636 }, "id": "NKOCPLAKPK_218", "locus": "GCA003260355_00177" }, { "type": "cds", "sequence": "contig_1", "start": 90691, "stop": 91023, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATCCGAGAGCTTGAAAACAAAAACTGGATTCATTCTTTGATAATTTATCTCAGCTGGGAAAATGTACTGAAGATTATCGTTTTTCTCCGCATAAAAATAAACCTGGAGATTCTACAGATCCTTCGGATTCCTCTCCTGAAGGGACTCCAGAGTCGAATGAATCAGTATTGGAGCAGTTTTATGAAGCTTGGAAGTCTCTTGATGGGATGCAAAGTGAGGATCCAGATTGTGGATTTAAGGAAGGTGCTAGTATTTATGGTCCTTTGTGTGAAAGTGCGCTTCCTCCTTATGAAAACTGAGGAATGTCGTGGATTAAACTTGAAATTGTGA", "aa": "MIRELENKNWIHSLIIYLSWENVLKIIVFLRIKINLEILQILRIPLLKGLQSRMNQYWSSFMKLGSLLMGCKVRIQIVDLRKVLVFMVLCVKVRFLLMKTEECRGLNLKL", "aa_hexdigest": "4e49e2abcec6680410bbf6961b41e577", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13145.126099999987, "isoelectric_point": 10.158363914489744 }, "id": "NKOCPLAKPK_219", "locus": "GCA003260355_00178" }, { "type": "cds", "sequence": "contig_1", "start": 90996, "stop": 91262, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCGTGGATTAAACTTGAAATTGTGAAGGTCAATAAAGATGCAACGCCACCTACCTTTAAGTTAAAGTCGAGCGGAACAGAGAGAAAGCTTGCTGCTTCTGAAGAGGGAAAAGAGCGAACACTGACTATGACCAAAGAAACTCTTGATAAGATTCGTGGTCTTTTGAATGGAGGAGCTAAGAAGGTTTTGAGTGAGAAAAAAGATTTTAATCAACAGATGGAGGATTTTCAGAAAGCAGGTCTGTGTGATAGAAATGTCCTGTGA", "aa": "MSWIKLEIVKVNKDATPPTFKLKSSGTERKLAASEEGKERTLTMTKETLDKIRGLLNGGAKKVLSEKKDFNQQMEDFQKAGLCDRNVL", "aa_hexdigest": "bfb6629dd0be44e671599d3e7b47f69b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9913.393799999996, "isoelectric_point": 9.478867149353029 }, "id": "NKOCPLAKPK_220", "locus": "GCA003260355_00179" }, { "type": "cds", "sequence": "contig_1", "start": 91500, "stop": 91769, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTTATTCAGATTTTTTATTCTTTTTAAATGAAAAAAAGCTTGCTCCCAAGACGCAGTCTCAGGTTGAAGAGGAGAAACAGGCTAGACAAGATGTGATCTCTTCCCATAATAGAACGTGGAAATTTACAAGTATTCAGTCTGTACTTTTTGCTCGGAAGACTATCTGGAAAAATCTGAATTCCAAGCTAGATGAATATCAAAAGGAACAAAATGAGGATTTTTTGGGATATTTGGTGACAGGAAAGGGAATTTATAAGTGGATATGA", "aa": "MSYSDFLFFLNEKKLAPKTQSQVEEEKQARQDVISSHNRTWKFTSIQSVLFARKTIWKNLNSKLDEYQKEQNEDFLGYLVTGKGIYKWI", "aa_hexdigest": "d8d20e95e8f588af7fe024fdaf8cee46", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10641.946799999994, "isoelectric_point": 9.187727928161618 }, "id": "NKOCPLAKPK_221", "locus": "GCA003260355_00180" }, { "type": "cds", "sequence": "contig_1", "start": 91776, "stop": 92075, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGGCTTTATCCCTTCGATCAAAGATGCTGCCAATAAGCTCCATGAGGAAGCAATGCTCAAAAAAGAGTCAAATACTTGGAAAGGAATAGAAGCTTGGATCAGTAAATTTGCTTCTATGCAAGATTATGCTAATCTTTTTGAAAAAGGGTTTGATCATCCGAATGGCTATACGCTAGATGCGATCATAGGAAAAGGGAATACCTTGCAGAAGATCCTTCTTAGTGGGAAATCTGTTGTGGAGAATGATGGACTGAGACCAATTATGGCAGCAGCAATGATTGCAAATCTTAGGCAATGA", "aa": "MGFIPSIKDAANKLHEEAMLKKESNTWKGIEAWISKFASMQDYANLFEKGFDHPNGYTLDAIIGKGNTLQKILLSGKSVVENDGLRPIMAAAMIANLRQ", "aa_hexdigest": "bcae967b1da1d3ab9de95011f2ac2f3c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10953.54369999999, "isoelectric_point": 7.986424064636231 }, "id": "NKOCPLAKPK_222", "locus": "GCA003260355_00181" }, { "type": "cds", "sequence": "contig_1", "start": 92094, "stop": 92297, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTCAGGTAAGGATAATCAGGGACTTTGGGTGAAGTGTTTGCTGGGAGATGCACACTATAAGAGATATAGAGCTATTAGAGCTGATGTCTTAAAAAAGATAAAGTCTGGAGTGAAGAATGCTGATCAGCTTCAGGATCAGCTAAAAAAATCAGAGGTGGATTATATCGTCAATAATATCAGAAATGCAAATGGGACAGAATGA", "aa": "MSGKDNQGLWVKCLLGDAHYKRYRAIRADVLKKIKSGVKNADQLQDQLKKSEVDYIVNNIRNANGTE", "aa_hexdigest": "e791328c25a1ce1f9f9968b6771e3468", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7619.614100000001, "isoelectric_point": 9.561386680603025 }, "id": "NKOCPLAKPK_223", "locus": "GCA003260355_00182" }, { "type": "cds", "sequence": "contig_1", "start": 92532, "stop": 93071, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGCGAAAACTCCAGAAAATCATAGGAAACTTAAAGTGGCTATGACCTATGTGATGCTCAGTGGTATCATGAATAGATATGGTGATAAAGCAATGAGGAAGTGGTTTGATGCTTTGGCGAGAAACTATATGTTGCCGACGACGCTTTTTGCTAAAAAATGGGAAAATCAAGAATATGCAGCTCATCTGCTCAATCAAGTATTCCCTCCGTCAGATGAGAAAAATTTTGCTCGTGCTACAGGATATAAATTATCAGATTTTGATAGCTCTTCTCCAAATATTCCTTATGGTAAGTTGCTTGATAACTTGGATGTTCGATGGAGAGAAAATGAGAAATCAATTGATAATTATTTTCTCAAACTTAAGAGCTCAGATTGTGCTCATGATCCTATCCTCAAGGCGGTTCAAGATAAACTTTGGGAGAGGAGTGAGGATAACCTCGATGGAGACTGGGCTTCAAATAGTGGACTTACGGGACACTATGCGATGTTAGCATCTCCTTCTACTATTTCGAGGAATAAGTCCTATGATCGTTCATGA", "aa": "MAKTPENHRKLKVAMTYVMLSGIMNRYGDKAMRKWFDALARNYMLPTTLFAKKWENQEYAAHLLNQVFPPSDEKNFARATGYKLSDFDSSSPNIPYGKLLDNLDVRWRENEKSIDNYFLKLKSSDCAHDPILKAVQDKLWERSEDNLDGDWASNSGLTGHYAMLASPSTISRNKSYDRS", "aa_hexdigest": "67f0bb4181b92332cde5ecb76110b5d3", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 20639.067099999997, "isoelectric_point": 8.836762046813966 }, "id": "NKOCPLAKPK_224", "locus": "GCA003260355_00183" }, { "type": "cds", "sequence": "contig_1", "start": 93261, "stop": 93533, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAAAAATTATGGGACAAAAAAGGCTATCTCGTTTACAATCCATGATGAATCTGATACGCAAAAGACTTTTTCGTCTTCTCCTGCTGTGTATAAACAAACAGATGCAAGGGATGTTTTGTGGTATCTCTTTAAGGGGAAAGTTTTGAGATTAAACTCTGCTCCTCCTCCAAAGCAGTTTGAAGATGTTTTGAATTTTTTCATGGATTATTTTAGTAAAGGTCATGTTTTGTCAACAATTGCTCAGGATTCTACCTTGAAAAAGGTGTTTTGA", "aa": "MKNYGTKKAISFTIHDESDTQKTFSSSPAVYKQTDARDVLWYLFKGKVLRLNSAPPPKQFEDVLNFFMDYFSKGHVLSTIAQDSTLKKVF", "aa_hexdigest": "ef8d25d1dfda4033c739385da19a939d", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10344.699299999995, "isoelectric_point": 9.397894859313965 }, "id": "NKOCPLAKPK_225", "locus": "GCA003260355_00184" }, { "type": "cds", "sequence": "contig_1", "start": 93748, "stop": 93933, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATATGGGAATTTCTGATACTCCTCAACTTTCCGGATTTGATGAAGATTTTTCTAGCAATATTTATAATCAGGTTTATCACACCGCAGCTTAGCCATGTGAGAGATATTTTATTGCTTTTATTCAAAGAACAACTTGCTTTTCTTAGAGAAAACTCTAATCTAAATCTGTTTTTTAGATTGTAG", "aa": "MIWEFLILLNFPDLMKIFLAIFIIRFITPQLSHVRDILLLLFKEQLAFLRENSNLNLFFRL", "aa_hexdigest": "f5741439cc466beada836319dd599865", "start_type": "ATG", "rbs_motif": "TTCTA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7452.9941999999955, "isoelectric_point": 8.363628578186038 }, "id": "NKOCPLAKPK_226", "locus": "GCA003260355_00185" }, { "type": "cds", "sequence": "contig_1", "start": 93944, "stop": 94150, "strand": "+", "frame": 2, "gene": null, "product": "Mur ligase central domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A948C409" ], "nt": "ATGAAGACTTGGTTGTTATTTCAGTATTATTGTTTTCTTGGGCGATGTGCTAGGCATTATCTCAAGAAATATCAGGTCTTCACTATAGGAATCAATGGAAGTGTGGGTAAAACTTCATGCCGTATGATTATTCATCAAACTTTGCAAAAAGCACTTCCTGAGCAGAGAATTTATACTTCTCCAAAAAATTTCAATGGCGAACTCTGA", "aa": "MKTWLLFQYYCFLGRCARHYLKKYQVFTIGINGSVGKTSCRMIIHQTLQKALPEQRIYTSPKNFNGEL", "aa_hexdigest": "d1fe74077e3d22ed04c133da7f1d233f", "start_type": "ATG", "rbs_motif": "TTGTA", "psc": { "uniref90_id": "UniRef90_A0A948C409", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.632, "score": 82.0, "evalue": 2.78e-19, "valid": false, "product": "Mur ligase central domain-containing protein", "uniref50_id": "UniRef50_A0A948C409" }, "pscc": { "uniref50_id": "UniRef50_A0A948C409", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A948C409" ], "product": "Mur ligase central domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_227", "locus": "GCA003260355_00186" }, { "type": "cds", "sequence": "contig_1", "start": 94151, "stop": 94483, "strand": "+", "frame": 2, "gene": null, "product": "Mur ligase central domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3D0UUH2" ], "nt": "ATGTCCCTCTCTATTCTCCAGGTAGAACGTCGAGAACCTCAGATTTTTACTTTTCTGAAAACGGCGATTCTGGCACTCAAAAAAGCTTTTTTCTCAAAGAAGGAGTATGATATTATTGTGCTAGAATATGGGATTGATAGACCAAAGGAGATGGAGTTTTTGCTAAGGATTAATAAGCCACATATCGGCGTTTTTACGGCGATTGATGCGGTGCATTCTGAGCAATTTGGTAATCCAGCCGAGATTGCTAGAGAAGAAGTAAAAATGGTAAAAAATACGAGAGAAATGGCTTTTCTGAATTTGGATGATCTGTATGCTCAGCAGCTCGTTTGA", "aa": "MSLSILQVERREPQIFTFLKTAILALKKAFFSKKEYDIIVLEYGIDRPKEMEFLLRINKPHIGVFTAIDAVHSEQFGNPAEIAREEVKMVKNTREMAFLNLDDLYAQQLV", "aa_hexdigest": "ca81df0e84b3157f50ed60b37e765f8a", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A948C556", "query_cov": 0.9727272727272728, "subject_cov": 0.8842975206611571, "identity": 0.701, "score": 150.0, "evalue": 3.73e-45, "valid": false, "product": "Mur ligase central domain-containing protein", "uniref50_id": "UniRef50_A0A3D0UUH2" }, "pscc": { "uniref50_id": "UniRef50_A0A3D0UUH2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3D0UUH2" ], "product": "Mur ligase central domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_228", "locus": "GCA003260355_00187" }, { "type": "cds", "sequence": "contig_1", "start": 94592, "stop": 94786, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCTGACGGATCTTGCTGTGCAGCTCAAGAAACAGTTGTTAAACCTTCAAACAAATCTTTTGGGAAAACCGAATTATGGCTATCTTACGGTTGCTTTGGTTATCGCTCAGGTATTGGCGTTGAAGTTTGGTGGGAAGGAGCTCGATATGGATGCTTTGTTGGCAGAGCCTTTGATTTTTCAGCTTCAGCCGTGA", "aa": "MLTDLAVQLKKQLLNLQTNLLGKPNYGYLTVALVIAQVLALKFGGKELDMDALLAEPLIFQLQP", "aa_hexdigest": "16a9c9e85cc4730ec4efa8aaac0963df", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7052.3843, "isoelectric_point": 6.000117301940917 }, "id": "NKOCPLAKPK_229", "locus": "GCA003260355_00188" }, { "type": "cds", "sequence": "contig_1", "start": 94859, "stop": 95074, "strand": "+", "frame": 2, "gene": null, "product": "Mur ligase C-terminal domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A9E5L0A4" ], "nt": "ATGAGAAAGCTCATTGATACAACCTTGATCCTTAATAAGTCGCTTCCTCAGCAGAGAAAGGTGATGCTGGTATTGGGAGATATGAGAGAGCTTGGAGATTTGACGGAAAAGGAGCATCGTTTGCTGGCTGGCTATGTGCAGCAGAGCGCAGATTTTGTGGTGCTACTTGGGACTTCAATGCATCAGTTTCTCGCTGACGAATTAGAAAAAATCTGA", "aa": "MRKLIDTTLILNKSLPQQRKVMLVLGDMRELGDLTEKEHRLLAGYVQQSADFVVLLGTSMHQFLADELEKI", "aa_hexdigest": "c938b7cf051e1efb5d025e1472095790", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A9E5L0A4", "query_cov": 0.9859154929577465, "subject_cov": 0.8045977011494253, "identity": 0.557, "score": 71.2, "evalue": 9.47e-15, "valid": false, "product": "Mur ligase C-terminal domain-containing protein", "uniref50_id": "UniRef50_A0A9E5L0A4" }, "pscc": { "uniref50_id": "UniRef50_A0A9E5L0A4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A9E5L0A4" ], "product": "Mur ligase C-terminal domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_230", "locus": "GCA003260355_00189" }, { "type": "cds", "sequence": "contig_1", "start": 95387, "stop": 95599, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00224.27" ], "nt": "GTGTTTGGGATGAGATTGACAAAGATTATTGCTACCGTGAGAGATGATTATGATAAAGGAAAAATTGTAGAGCTTTTTGATGCGGGAGTTGATGTTATTAGGATAAATTTTACTCATGCAACGCCGGAAACTTCGGAGGCTTTGATGTCTGAAATTAATAGATTGAACGCAGAAGAGAAAACTCATTTGAGTCTGCTTTTGGATACCAAATGA", "aa": "MFGMRLTKIIATVRDDYDKGKIVELFDAGVDVIRINFTHATPETSEALMSEINRLNAEEKTHLSLLLDTK", "aa_hexdigest": "c94fb92ba906d63051f623f6a85e111c", "start_type": "GTG", "rbs_motif": "TTATT", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00224.27", "name": "PK", "length": 67, "aa_cov": 0.9428571428571428, "hmm_cov": 1.0, "evalue": 5.622926217941297e-10, "score": 35.21512222290039, "start": 5, "stop": 70 } ], "seq_stats": { "molecular_weight": 7937.045199999994, "isoelectric_point": 5.207383918762206 }, "id": "NKOCPLAKPK_231", "locus": "GCA003260355_00190" }, { "type": "cds", "sequence": "contig_1", "start": 95666, "stop": 95845, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATTGATACCTCAAAACTTGTGCAGGATTCGGATGTTTTCTGTGATTATCCTGGCATTTTGACTGATGTTTCAGTTGGGCAGGAAATTATTATTGATTCTGGGCTTTTGGTCGTGGTCGTGGTTGAAATTACTTCAGATTATTTGGTTGTAAAAGCGCTTAATGATGCAGAAATCTGA", "aa": "MIDTSKLVQDSDVFCDYPGILTDVSVGQEIIIDSGLLVVVVVEITSDYLVVKALNDAEI", "aa_hexdigest": "79e533fb6f5b7b879e3409231560b7ca", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6371.2341000000015, "isoelectric_point": 4.0500284194946286 }, "id": "NKOCPLAKPK_232", "locus": "GCA003260355_00191" }, { "type": "cds", "sequence": "contig_1", "start": 95894, "stop": 96121, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00224.27" ], "nt": "ATGATAGAGAAAGATAAGACGGATATTCTTTTTGGTATAAAGATGGGAATTGCTTATGTGGCGGCTTCTTTTATCAGAACTGGGGAGAATGTTCAGGAAATTAGGAGATTCCTTGATGAAAATGGAGGAACAGGTGTGAAGATTATTTCTAAGATTGAAAATAAGGAAGCTTTGGAAAATTTGGAAGCTATTGTCAGATATTCTGATGGTATTATGATAGCGAGGTGA", "aa": "MIEKDKTDILFGIKMGIAYVAASFIRTGENVQEIRRFLDENGGTGVKIISKIENKEALENLEAIVRYSDGIMIAR", "aa_hexdigest": "2987eaafd99185847ad1fd7af478e31f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00224.27", "name": "PK", "length": 74, "aa_cov": 0.9866666666666667, "hmm_cov": 0.9864864864864865, "evalue": 7.037233578789366e-25, "score": 84.19512939453125, "start": 2, "stop": 75 } ], "seq_stats": { "molecular_weight": 8404.650299999994, "isoelectric_point": 5.384892082214355 }, "id": "NKOCPLAKPK_233", "locus": "GCA003260355_00192" }, { "type": "cds", "sequence": "contig_1", "start": 96137, "stop": 96637, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00224.27", "PFAM:PF02887.21" ], "nt": "ATGCCTATTCACGAATTGCCAGTATATCAAAAGAAAATTTTGGATATGTGTTTTGTCTATGGGAAGCCTGTGATTATTGCTACTGAGCTGATGAAGAGTATGGTGAGCAATCCTTTTCCAACAAGAGCTGAGGTAAGTGATGTGTATAATTCTGTCATTATGAGGGCGGATGCCGTGATGCTTTCTGATGAAACGGCCGTTGGAAAATATCCTGTGAAAGCTGTTCAATATATGGAAAAAACTGTAATGGAGGCAGAAAAGACAACCTGCAATAAGCACAAGGATTTTGACCTCCAGTCAGAAGAAGAGTCAGAAGTTTTGAAAAAGGCGCTTGCACGTTATGCTCTGATGCTTGCGGATGATATTCATGCAAAGGTCGTAGTAGCTTTCTCTTATTCTGGGAATCTCGCGAGGTATTTGTCTGCTTTTAAGCCTAATCAACCTGTAATTTCTTTTACTACAGAGGAGGTTTTGGACTATTCTTTTGGGATTAATTACTGA", "aa": "MPIHELPVYQKKILDMCFVYGKPVIIATELMKSMVSNPFPTRAEVSDVYNSVIMRADAVMLSDETAVGKYPVKAVQYMEKTVMEAEKTTCNKHKDFDLQSEEESEVLKKALARYALMLADDIHAKVVVAFSYSGNLARYLSAFKPNQPVISFTTEEVLDYSFGINY", "aa_hexdigest": "afc624a57078a51ec3fd42fdf8a127c7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00224.27", "name": "PK", "length": 74, "aa_cov": 0.4457831325301205, "hmm_cov": 1.0, "evalue": 1.4282746575690478e-27, "score": 93.0448226928711, "start": 2, "stop": 75 }, { "id": "PF02887.21", "name": "PK_C", "length": 47, "aa_cov": 0.28313253012048195, "hmm_cov": 1.0, "evalue": 3.0350735091505724e-09, "score": 33.44660949707031, "start": 110, "stop": 156 } ], "seq_stats": { "molecular_weight": 18781.551699999996, "isoelectric_point": 5.41257266998291 }, "id": "NKOCPLAKPK_234", "locus": "GCA003260355_00193" }, { "type": "cds", "sequence": "contig_1", "start": 96674, "stop": 96817, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCTGAAGATCAGGATATGGCGATTGCTGTTCTGAAGGAGAAAGCTTTTGTGAAAAAAGGAGATTATGTACTTTTTGTCGGAGAAAGGGTCGCTGGGAACTCTCGCCAACCACAGTTGAGGATTGTTCAGATTGATCAGTAA", "aa": "MSEDQDMAIAVLKEKAFVKKGDYVLFVGERVAGNSRQPQLRIVQIDQ", "aa_hexdigest": "43b4aa2d3891910d5be805cc0ac7620e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5322.081500000001, "isoelectric_point": 6.081510734558105 }, "id": "NKOCPLAKPK_235", "locus": "GCA003260355_00194" }, { "type": "cds", "sequence": "contig_1", "start": 96925, "stop": 97044, "strand": "+", "frame": 1, "gene": "dnaJ", "product": "DnaJ domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A948C4P0" ], "nt": "ATGGATTTTGATCCAAAAAAGAATTATTATGAGATTTTGGGTGTGAGTGAAACTGCTACCGCAGATGAGATAAAAAAGACCTTTAGAAAGCAGGCTGTGAAGTATCATCCGGACCGTTGA", "aa": "MDFDPKKNYYEILGVSETATADEIKKTFRKQAVKYHPDR", "aa_hexdigest": "0ab7dbaeaeb67cb6567f2e5fa6877ec5", "start_type": "ATG", "rbs_motif": "TTGTA", "psc": { "uniref90_id": "UniRef90_A0A948C4P0", "query_cov": 1.0, "subject_cov": 0.975, "identity": 0.769, "score": 67.8, "evalue": 1.89e-14, "valid": false, "product": "DnaJ domain-containing protein", "uniref50_id": "UniRef50_A0A948C4P0" }, "pscc": { "uniref50_id": "UniRef50_A0A948C4P0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A948C4P0" ], "product": "DnaJ domain-containing protein" }, "genes": [ "dnaJ" ], "id": "NKOCPLAKPK_236", "locus": "GCA003260355_00195" }, { "type": "cds", "sequence": "contig_1", "start": 97073, "stop": 97381, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGGCTTATCAAGTGCTTTCTGACGATCAGAAAAGGCAACAATATGATATGTATAGGAAAGGAGGTTTTGGAGCTTGAGGCGTTGATTTCTGAGGTTTTTGAGGAGGAAGTGGTGCTCAGTTTGACTTCTGAGGATTTGGCGATATTGGCGATCTTCTCTGAGGAATGTTTGGAGGCGGGTTTTGAACTGGAGGATCGAGGACGAGAAAACCAACGAAAGGGACAGATTTGGAGAAGCATATTGAGATTACTTTTGAAGAGTCCTATCTTTGAGTCAAAAAGAAAATTGCCTATACGAGAGCGGTAA", "aa": "MRLIKCFLTIRKGNNMICIGKEVLELEALISEVFEEEVVLSLTSEDLAILAIFSEECLEAGFELEDRGRENQRKGQIWRSILRLLLKSPIFESKRKLPIRER", "aa_hexdigest": "c7200d6826e6a9d65f89f750473617f0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11892.869599999982, "isoelectric_point": 5.633619499206542 }, "id": "NKOCPLAKPK_237", "locus": "GCA003260355_00196" }, { "type": "cds", "sequence": "contig_1", "start": 97570, "stop": 97827, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01556.23" ], "nt": "ATGCTAGATTTGGAAATTCCCGCGGGTATAAAGTCTGGAAGTTTCCTCAAGTATGCTGGAAGAGGAGATGATGGAGCAGGAGATGTCCCTTCTGGAGACCTTTATGTGAAGATTCTGGTGCAGGATTCCACGAAATATGCGAGGAAGACGGATGATCTTTATGTAAAAGCTGAAGTTTCCCTTTTTGATTTGGTCCTGGGAGGAGAGGTCGAAGTGCCGCATCCTGAGGGAAAAATGACCGTAAAAATCCCTAAATGA", "aa": "MLDLEIPAGIKSGSFLKYAGRGDDGAGDVPSGDLYVKILVQDSTKYARKTDDLYVKAEVSLFDLVLGGEVEVPHPEGKMTVKIPK", "aa_hexdigest": "55dda0168b351ae5b1c2869b026c4ce5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01556.23", "name": "DnaJ_C", "length": 84, "aa_cov": 0.9882352941176471, "hmm_cov": 1.0, "evalue": 1.6544567005752144e-22, "score": 76.5870132446289, "start": 2, "stop": 85 } ], "seq_stats": { "molecular_weight": 9153.3994, "isoelectric_point": 4.984916114807128 }, "id": "NKOCPLAKPK_238", "locus": "GCA003260355_00197" }, { "type": "cds", "sequence": "contig_1", "start": 97824, "stop": 98000, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACTCAAGTAGGGCAAAAAGTCCGTGTAGCGGCAAAGGGTTTTGGAGAAAAGGGGCTTTTCAAGTCAAAAGGAGATATGATTGTAGAACTTCAGATTTCTGTGCCAAAAAAATTGACGAAAGAGCAGGAGAAGCTTCGAAAAGAGTTGCAAAAACTAAATTAGAAAAAATCTGA", "aa": "MNSSRAKSPCSGKGFWRKGAFQVKRRYDCRTSDFCAKKIDERAGEASKRVAKTKLEKI", "aa_hexdigest": "7e3ad52bcb0847834a81fe79652e505e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6613.597600000001, "isoelectric_point": 10.249586677551267 }, "id": "NKOCPLAKPK_239", "locus": "GCA003260355_00198" }, { "type": "cds", "sequence": "contig_1", "start": 98192, "stop": 98938, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00013.35", "PFAM:PF01138.27" ], "nt": "TTGGCAGAAAAAGCGCTTGAGAATATGATTCCAAGCAAAGAAGAATTCCCATATTCTATCAGAGTGGTTTCAGAATGTCTTGGCTCTGGAGGTTCAACCAGTATGGGATCCGTATGTGGTTCTACCCTTGCCCTTATGGATGCTGGAGTTCCGCTCAAGAAGCCAGTTGCAGGAATTGCGATGGGACTGATGACTGAAACCGATGAAAATGGAGCAGTAACCAAGCATATGGTGCTTAATGACCTTATGGCAACTGAGGATTTTACTGGAGATATGGACTTCAAAGTCGCAGGAACAAAAAAAGGAATCACCGCAATCCAGCTTGATACAAAACTCAAAGGTCTGACTTTGGATATTGTGCATGAAACCATTGATAGAGCAAGTGAGGGATACAAAGAGATTATGGATTTTATGCTAGAAACTATCCCAACTCCGAGAGCTCAAGTCAAAGAATATGCTCCAAAGATTCATGTTTTCAAAATCAATCCTGAGAAAATCAAGGAAGTGATTGGAAAAGGTGGAGAGATGATTGATAAAATTATTGAGCAGTGCGATAATATCAAGATTGACTTTGAAGATGACGGAACCTGCTTCCTTACCCATGAAAAGCAAGAGATGATTGAAAAGGCTAAAAATCTGATCCTTGAGATTGCAACAGATCTTGAGGTAGGACAAGTCTTTGACGCAAAAATCGTAAGAGTAGAAGCTTACGGACTCTTCGTTCAGCTTCCAAAGGGAAAGCAGTGA", "aa": "MAEKALENMIPSKEEFPYSIRVVSECLGSGGSTSMGSVCGSTLALMDAGVPLKKPVAGIAMGLMTETDENGAVTKHMVLNDLMATEDFTGDMDFKVAGTKKGITAIQLDTKLKGLTLDIVHETIDRASEGYKEIMDFMLETIPTPRAQVKEYAPKIHVFKINPEKIKEVIGKGGEMIDKIIEQCDNIKIDFEDDGTCFLTHEKQEMIEKAKNLILEIATDLEVGQVFDAKIVRVEAYGLFVQLPKGKQ", "aa_hexdigest": "104c393c609303437894b54a7fee6930", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_W4V913", "query_cov": 0.9838709677419355, "subject_cov": 0.8243243243243243, "identity": 0.525, "score": 233.0, "evalue": 4e-73, "valid": false, "product": "polyribonucleotide nucleotidyltransferase", "ec_ids": [ "2.7.7.8" ], "uniref50_id": "UniRef50_A0A944BT45", "kegg_orthology_id": "K00962" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00013.35", "name": "KH_1", "length": 66, "aa_cov": 0.23790322580645162, "hmm_cov": 0.9696969696969697, "evalue": 1.262735119398824e-10, "score": 37.42031478881836, "start": 158, "stop": 216 }, { "id": "PF01138.27", "name": "RNase_PH", "length": 50, "aa_cov": 0.20161290322580644, "hmm_cov": 1.0, "evalue": 2.9425230903565577e-09, "score": 33.87833786010742, "start": 2, "stop": 51 } ], "seq_stats": { "molecular_weight": 27235.330200000015, "isoelectric_point": 4.90744457244873 }, "id": "NKOCPLAKPK_240", "locus": "GCA003260355_00199" }, { "type": "cds", "sequence": "contig_1", "start": 98948, "stop": 100189, "strand": "-", "frame": 3, "gene": null, "product": "polyribonucleotide nucleotidyltransferase", "db_xrefs": [ "EC:2.7.7.8", "GO:0003723", "GO:0004654", "GO:0006396", "GO:0006402", "KEGG:K00962", "SO:0001217", "UniRef:UniRef50_A0A288DF36", "UniRef:UniRef90_A0A328EJ59" ], "nt": "ATGGTCGTAAAAAAACAATTTGAAATCGCTCGTCAATCTACCGTGCGATATGGAAAACCAGTTTCCTTTGAAATGGGGAAACTTGCAGTGCAAGCTGATGCAAGCATTAGAATGCAAATGGGAGAAAATGTGCTTCTTTATTCAACCGTAATGGAGAAAAATCCAAGAGAAGGCATGGACTTTTTACCACTCATGATTGATATGAGAGAAAGTTATTCCGCTGCTGGAAGGATCGGAGGTGCGGTCTATAGAAGGAGGGAAGGAAGACCTTCTGATCAAGCAATTCTCTATGCGAGACTTACCGATAGAGCGCTCAGACCGATGTTTCCAAAGGGAATGATCAATGATGTCGTAATCTCCGTGACTCCACTTGCACTTGATCATGAAATCAGCCTTGGAGTGATGGATATTATCGGAGCTTCGCTTTCTATTATGGCGGCGGGGATTCCGTTTGATGGTCCCGTAGGTGCAGCACAGATCGGCTATCTTGATGGGGAATTTATCGTAAATCCTACCAAAGAACAGTTGGAAAAATCTGAATTAAATCTTTTGGTAGCAGGAAAGAAAGGGTCTATCAATATGATTGAATGTGAATCGCTTGAATTCCCTGATGAGCTGATGAAACAAGCTTTCACGCTTGGACAGAAAGTAATTGATGAGTCCTGCGATCTTCAGCTTGATTTCTTGAAGACCTTAGACATTCAGCAGAAAGAAATCACTTTCAATAAGCCAAGTGAAGCAACGATGGCGTTTGTAAAAACACTCTTGACCGATGAAAAACTCCAAGCCCTTGCTGGAAATACCAAAGTACCTTTCAACGATCTTTTCAGTCAGTATGAAAAAGAAGCTCTTGAAGCAGCAAAAGAAAAAGCTGAATCCGAAGACGCAGAAGAATACACAGAAAGTAAAATCAAGATGGCAGTTTTCAATACGGTGAAAGCTTTCATCAGAAGCAGAACTCTCGCTACAGGAAAGAGAATTGACGATAGACAAATCAAAGATATTAGACCGCTTTTCTGTGAGACGGATAATCTCCCAAGAGTACATGGTTCTGGACTTTTCCGAAGAGGAGATACGCAGGTGCTTTCTACCGTAACACTCGGAGGTCCAAAAGATTACCTTGTCCTTGAAGATATGGAGCATAACGATGTTGAGCAGAGATATTTCCATCACTATAACTTTCCTCCTTTCTCTACTGGAGAAGCAAAAGCCATGAGAGGAACAGGAAGAAGAGAAATCTGA", "aa": "MVVKKQFEIARQSTVRYGKPVSFEMGKLAVQADASIRMQMGENVLLYSTVMEKNPREGMDFLPLMIDMRESYSAAGRIGGAVYRRREGRPSDQAILYARLTDRALRPMFPKGMINDVVISVTPLALDHEISLGVMDIIGASLSIMAAGIPFDGPVGAAQIGYLDGEFIVNPTKEQLEKSELNLLVAGKKGSINMIECESLEFPDELMKQAFTLGQKVIDESCDLQLDFLKTLDIQQKEITFNKPSEATMAFVKTLLTDEKLQALAGNTKVPFNDLFSQYEKEALEAAKEKAESEDAEEYTESKIKMAVFNTVKAFIRSRTLATGKRIDDRQIKDIRPLFCETDNLPRVHGSGLFRRGDTQVLSTVTLGGPKDYLVLEDMEHNDVEQRYFHHYNFPPFSTGEAKAMRGTGRREI", "aa_hexdigest": "f229ff8ece14b4f3e35be25a3239ec30", "start_type": "ATG", "rbs_motif": "TTTTT", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 98645, "stop": 100189, "observations": { "mutation": [ 98950 ], "stop": [ 98950 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EJ59", "query_cov": 1.0, "subject_cov": 0.5808720112517581, "identity": 0.985, "score": 793.0, "evalue": 9.59e-285, "gene_start": 1, "gene_end": 413, "reference_start": 1, "reference_end": 413, "reference_sequence": "MVVKKHFEIARQSTVRYGKPVSFEIGKLAVQADASIRMQMGENVLLYSTVMEKNPREGMDFLPLMIDMRESYSAAGRIGGAVYRRREGRPSDQAILYARLTDRALRPMFPKGMINDVVISVTPLALDHEISLGVMDIIGASLSIMAAGIPFDGPVGAAQIGYLDGEFIVNPTKEQLEKSELNLLVAGKKGSINMIECESLEFPNELMKQAFTLGQKVIDEACDLQLDFLKTLDIQQKEITFNKPSEATMAFVKTLLTDEKLQALAGNTKVPFNDLFSQYEKEALEAAKEKAESEDAEEYTESKIKMAVFNTVKAFIRSRTLATGKRIDDRQIKDIRPLFCETDNLPRVHGSGLFWRGDTQVLSTVTLGGPKDYLVLEDMEHNDVEQRYFHHYNFPPFSTGEAKATRGTGRREIGHGRLAEKALENMIPSKEEFPYSIRVVSECLGSGGSTSMGSVCGSTLALMDAGVPLKKPVAGIAMGLMTETDENGAVTKHMVLNDLMATEDFTGDMDFKVAGTKEGITAIQLDTKLKGLTLDIVHETIDRASEGYKEIMDFMLETIPTPRAQVKEYAPKIHVFKINPEKIKEVIGKGGEMIDKIIEQCDNIKIDFEDDGTCFLTHEKQEMIEKAKNLILEIATDLEVGQVFDAKIVRVEAYGLFVQLPKGKQGLCHVSNLGQKFTDGLEKHFKVGDTINVVISQIGFDGKIAVKRKLG" }, "paralog": false, "identity": 0.9863813229571985, "score": 994.0, "evalue": 0.0, "description": "Internal stop codon at 98950. Nonsense mutation around 98950.", "psc": { "uniref90_id": "UniRef90_A0A328EJ59", "product": "polyribonucleotide nucleotidyltransferase", "ec_ids": [ "2.7.7.8" ], "uniref50_id": "UniRef50_A0A288DF36", "kegg_orthology_id": "K00962", "go_ids": [ "GO:0003723", "GO:0004654", "GO:0006396", "GO:0006402" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EJ59", "EC:2.7.7.8", "UniRef:UniRef50_A0A288DF36", "KEGG:K00962", "GO:0003723", "GO:0004654", "GO:0006396", "GO:0006402" ] }, "pscc": { "uniref50_id": "UniRef50_A0A288DF36", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A288DF36" ], "product": "Polyribonucleotide nucleotidyltransferase" } }, "id": "NKOCPLAKPK_241", "locus": "GCA003260355_00200" }, { "type": "cds", "sequence": "contig_1", "start": 100427, "stop": 100822, "strand": "+", "frame": 2, "gene": null, "product": "Rhodanese domain-containing protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBF42B6", "UniRef:UniRef100_A0A328EFV0", "UniRef:UniRef50_A0A328EFV0", "UniRef:UniRef90_A0A328EFV0" ], "nt": "ATGAAGAAAACCTCACTTCTCCTTACCATTATCCTTGCTCTTGTGGGGGCACTTTTTGGGGTATGGTATTTTAACGCTTCTCATTCTTGTAGTGCTTTTTCTGCTGAATGTAAGGAAATCCCTGTTCAGCAACAGGAAAAAATTTATCTCGATGTGCGTGAAGAAGAGGAATGGCTTGCTGGGCATGTAAAGGGAGCTTTACACATCAAGATGAGCGATATTTTTGCTGGGAACTATGAGCAAATTCCACAAACTGAACCAGTCTATGTATATTGCCGTTCTGGGCGCCGTGCAGGTGAAGTGATTCGCTTTTTAGAGCAAAAAGGATTCAAAAATCTGATCAATGCTGGTGGTTTGCGTGATCTAGAAGGAGTAGAAATCGTAGAAGGTGAGTAA", "aa": "MKKTSLLLTIILALVGALFGVWYFNASHSCSAFSAECKEIPVQQQEKIYLDVREEEEWLAGHVKGALHIKMSDIFAGNYEQIPQTEPVYVYCRSGRRAGEVIRFLEQKGFKNLINAGGLRDLEGVEIVEGE", "aa_hexdigest": "85ad7ac5a0cb5be737e42103ae9e2c22", "start_type": "ATG", "rbs_motif": "TTATT", "ups": { "uniparc_id": "UPI000DBF42B6", "uniref100_id": "UniRef100_A0A328EFV0", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBF42B6", "UniRef:UniRef100_A0A328EFV0" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EFV0", "uniref90_id": "UniRef90_A0A328EFV0" }, "psc": { "uniref90_id": "UniRef90_A0A328EFV0", "product": "Rhodanese domain-containing protein", "uniref50_id": "UniRef50_A0A328EFV0" }, "pscc": { "uniref50_id": "UniRef50_A0A328EFV0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EFV0" ], "product": "Rhodanese domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_242", "locus": "GCA003260355_00201" }, { "type": "cds", "sequence": "contig_1", "start": 101091, "stop": 101204, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00132.30" ], "nt": "GTGGATCCTTCAGCACAGATTGATCCTTCTGTTGTTGTTCCAGAGGGCTGTGTTATTGGACCAAATGTCGTTATCGCAAAGGGGGCTGTTTTAGGAAGAAATGTTCATATTTGA", "aa": "MDPSAQIDPSVVVPEGCVIGPNVVIAKGAVLGRNVHI", "aa_hexdigest": "75539edc2c00e58c2d0dc85f30e8c136", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00132.30", "name": "Hexapep", "length": 29, "aa_cov": 0.7837837837837838, "hmm_cov": 1.0, "evalue": 1.9401402588473497e-09, "score": 33.338478088378906, "start": 5, "stop": 33 } ], "seq_stats": { "molecular_weight": 3752.365, "isoelectric_point": 5.362383842468262 }, "id": "NKOCPLAKPK_243", "locus": "GCA003260355_00202" }, { "type": "cds", "sequence": "contig_1", "start": 101384, "stop": 101596, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTGCTTCGTTACAGATTTTCGCATTCTTTCAGATGTTTGCTCCGTCCCAGATTTTCGTATTCTTTCAGATGTTTGCTCATTCTCTGATGTTCGCATTCTTTCAGATGTTTGTTTCGTCACAGATTTTCACATTCTTTCAGATATTTGATCCGCTCGAGATTTTCGCTCATTCTCAGATTTCCACTCATTCTCAGATTTGTACTCCATAG", "aa": "MFASLQIFAFFQMFAPSQIFVFFQMFAHSLMFAFFQMFVSSQIFTFFQIFDPLEIFAHSQISTHSQICTP", "aa_hexdigest": "c92199bf0ac64ca4435964203d8cd520", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8282.629799999992, "isoelectric_point": 5.70074634552002 }, "id": "NKOCPLAKPK_244", "locus": "GCA003260355_00203" }, { "type": "cds", "sequence": "contig_1", "start": 101636, "stop": 101920, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTCGCTCATCGTCAGATTTTCACATTATCTCAGATTTCCGCATTATTTCAGATTTTCGCATTATTTCAGATGCTCGCTCATTTTCAGATTTTCGCTCATTCTCAAATGCCCGCTCGTCGTCAGATTCTCACATTATCTCAGATTTTCACATTATTTCAGATTTCCGCATCAAGTCAGATCTCCGCATTATTTCAGATTTTTGCATTCTTTCAGATGTTTGCTTTGTTACAGATCTCCACATTATTTCAGATTTCCACTCATTCTCAGATTTGTACTTCATAG", "aa": "MLAHRQIFTLSQISALFQIFALFQMLAHFQIFAHSQMPARRQILTLSQIFTLFQISASSQISALFQIFAFFQMFALLQISTLFQISTHSQICTS", "aa_hexdigest": "b34e8bd1e7b3c6b55a4a0ca98a52959e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10820.65529999999, "isoelectric_point": 11.700254249572755 }, "id": "NKOCPLAKPK_245", "locus": "GCA003260355_00204" }, { "type": "cds", "sequence": "contig_1", "start": 101991, "stop": 102194, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATAGGCTATCTTCAGCAAGAGGAAAGAGTAAATGAGGAGCAAGAGGAAAGAGTAAATGAGGAGCAAGAGGAAAGAGTAGATGAGGAGCAAGAGGAAATAGAGAATGAGAAGCAAGAGGAAAGAGTAGATGAGAAGCAAGAGGAAAGAGTAAATGAGGAGAAAAAGAGCTTTTGGAAAAGACTTTTTGGAAAAAATGATTAA", "aa": "MIGYLQQEERVNEEQEERVNEEQEERVDEEQEEIENEKQEERVDEKQEERVNEEKKSFWKRLFGKND", "aa_hexdigest": "2a06e1a59e9853c0fbb6fdcae9071e70", "start_type": "TTG", "rbs_motif": "TTATT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8430.856899999995, "isoelectric_point": 4.378387260437012 }, "id": "NKOCPLAKPK_246", "locus": "GCA003260355_00205" }, { "type": "cds", "sequence": "contig_1", "start": 102327, "stop": 102728, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF09269.16" ], "nt": "ATGCTAAGAAAACTCGCTGAACTCCTCCAAAAAACTCCAGACAATGGCTATCACCCAGAAAGCATCTTTGATGAAACAACCTATTTCGAAGAACCTGAAACTGAAGATTTGACAATGGTCACAGAGATCACCGAGCAAGAGAAACCAACCTTGATCAAGGACGGGTATCTTGAAGCCCTAGAGAGTAGATTCCTAAAAGTTTGGTATCTCCAAAACCGCGAAATCTGCAAACTCGTCTTTATGATCCCTCGAGGCAACGACCAAGCCGAGCAATATTTTCGACAACAAATGCAAAGCAAAGGATTCCTAGAGATACTCTCATCTGAAGGGGTAATGAAGGGCGATGTGCTCAGAGTTAAAAGCTATTACGAAAACTTTGACGACAAATATATCCAATATTAA", "aa": "MLRKLAELLQKTPDNGYHPESIFDETTYFEEPETEDLTMVTEITEQEKPTLIKDGYLEALESRFLKVWYLQNREICKLVFMIPRGNDQAEQYFRQQMQSKGFLEILSSEGVMKGDVLRVKSYYENFDDKYIQY", "aa_hexdigest": "fb89b5e971fa5b27a49845b35a67d687", "start_type": "ATG", "rbs_motif": "TTATT", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF09269.16", "name": "DUF1967", "length": 56, "aa_cov": 0.42105263157894735, "hmm_cov": 1.0, "evalue": 1.939218151890367e-08, "score": 30.71022605895996, "start": 66, "stop": 121 } ], "seq_stats": { "molecular_weight": 15886.818499999994, "isoelectric_point": 4.666674613952637 }, "id": "NKOCPLAKPK_247", "locus": "GCA003260355_00206" }, { "type": "cds", "sequence": "contig_1", "start": 103068, "stop": 103289, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAAATGTTAAGGCAAAAGTCGCCGATTATCCTTTTACTACCCTGGTTCCAAATCTAGGCTCCGTATCCGTTGGTGATTTCAAGTTTAATGTAATTGACATCCCAGGACTCATTGAAGGAGCATCTGAGGGAAAGGGGCTAGGAAACGCTTTTCTCAGACACATTTTAAAAGCAAGAATCTTTGCCTTTGTAGCCGACCTTTCCAGATTTGAAGCCTGA", "aa": "MANVKAKVADYPFTTLVPNLGSVSVGDFKFNVIDIPGLIEGASEGKGLGNAFLRHILKARIFAFVADLSRFEA", "aa_hexdigest": "15368ac71d963fbd21911ede0c03945c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7825.991999999997, "isoelectric_point": 8.12071170806885 }, "id": "NKOCPLAKPK_248", "locus": "GCA003260355_00207" }, { "type": "cds", "sequence": "contig_1", "start": 103559, "stop": 103759, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGAAGAAGAGCTCCAGGAATCCCGTTTGGAGGCCCTTCATGAGGAGATGGCGGAGACTGAGGTTCAGTATACATTACCGCATCAAAGGACGAAAACACCCTTATTGAGTATAAATATAAAAAAAGTTTTAAAGCAGAACCTTGATTTCCAGGTAGGACCAAAGACCAATATGGAGCACACGGTGAGGACCTCCAACTAA", "aa": "MEEELQESRLEALHEEMAETEVQYTLPHQRTKTPLLSINIKKVLKQNLDFQVGPKTNMEHTVRTSN", "aa_hexdigest": "27fce927e283b3b0205af386c90774e0", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7733.682499999998, "isoelectric_point": 5.527330589294433 }, "id": "NKOCPLAKPK_249", "locus": "GCA003260355_00208" }, { "type": "cds", "sequence": "contig_1", "start": 103716, "stop": 103823, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01018.28" ], "nt": "ATGCAATTTTATGATGAAGTCAAAATAACCATCGAATCTGGAAAAGGAGGAGACGGAGTAGCAAGTGGAAGAAGAGCTCCAGGAATCCCGTTTGGAGGCCCTTCATGA", "aa": "MQFYDEVKITIESGKGGDGVASGRRAPGIPFGGPS", "aa_hexdigest": "019f61b5d43bfc5b8bc04231cd418ca0", "start_type": "ATG", "rbs_motif": "TTTA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01018.28", "name": "GTP1_OBG", "length": 32, "aa_cov": 0.9142857142857143, "hmm_cov": 1.0, "evalue": 1.0362419426720789e-07, "score": 28.142913818359375, "start": 4, "stop": 35 } ], "seq_stats": { "molecular_weight": 3581.9625000000005, "isoelectric_point": 5.971413612365722 }, "id": "NKOCPLAKPK_250", "locus": "GCA003260355_00209" }, { "type": "cds", "sequence": "contig_1", "start": 104194, "stop": 104316, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01195.25" ], "nt": "ATGAATCTTTCGGGAGAAAGTGTTGCTCCTTTGGCGAGGTTTTTTAAAATTTCAGCAGCGTCTATTTTGGTGCTTCATGATGAGATTGATTTCGTTACAGGAAGGATTGCTCTCAAATTCTGA", "aa": "MNLSGESVAPLARFFKISAASILVLHDEIDFVTGRIALKF", "aa_hexdigest": "d228f88b50ed1f32bbd38ac0be7c1285", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01195.25", "name": "Pept_tRNA_hydro", "length": 39, "aa_cov": 0.975, "hmm_cov": 1.0, "evalue": 2.8870714308528586e-11, "score": 39.99419021606445, "start": 1, "stop": 39 } ], "seq_stats": { "molecular_weight": 4378.098999999998, "isoelectric_point": 6.519000434875489 }, "id": "NKOCPLAKPK_251", "locus": "GCA003260355_00210" }, { "type": "cds", "sequence": "contig_1", "start": 104772, "stop": 104924, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATGGAGCGATTCAGTTTTATCAAGCGGCAAATGCTGAAGGGATTAAACCGATTATCGGTTTAGAAGTCGGGTTTGTCTTGAATGTTGATAATGCTCCAGCTGTTAATGCTATCGGAAGTATCTGTCTTTTGGCTAAAAATACTCAATGA", "aa": "MYGAIQFYQAANAEGIKPIIGLEVGFVLNVDNAPAVNAIGSICLLAKNTQ", "aa_hexdigest": "3d54db2c1a3715d3369166ca7f5dc007", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5222.000100000001, "isoelectric_point": 4.677076148986816 }, "id": "NKOCPLAKPK_252", "locus": "GCA003260355_00211" }, { "type": "cds", "sequence": "contig_1", "start": 104937, "stop": 105134, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGATTACGTCTTTTGCGGGTCAGCAGGGGGTTGCAGGTAGACCAAAGATTGATATTACTCTTTTGGAGAAATATAAGGAAGGAATTCTGGTTTTCTCTGGTGGAGTGGACTCGTGGATTGCGAAACTTTTGAGTAACGGCGAATCGCTTTCCAAGGCGGAAGAGATCTTTACTATGCTGAAGGATAAGCTCTGA", "aa": "MKITSFAGQQGVAGRPKIDITLLEKYKEGILVFSGGVDSWIAKLLSNGESLSKAEEIFTMLKDKL", "aa_hexdigest": "09a0cc16364e380f442d7f58647c7558", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7102.229899999998, "isoelectric_point": 8.008730125427245 }, "id": "NKOCPLAKPK_253", "locus": "GCA003260355_00212" }, { "type": "cds", "sequence": "contig_1", "start": 105219, "stop": 105581, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGCGGAAAGGGTTCAGGCTTCTTGTCTAGTAAGTAATATTTATATTTACCCAAAACCAGAGGATAAGCCTACGCAGGAATTGGCTATGGCGATAAAGGATAATCTAAAACTTTATGACCCTCAACATAGAGTCTTGACTACGGAAAATCATCTTATGACGGAAGAAGAAATTAGAAAAATCTGTCTGGAAAACGGCTATTCTGAAGCTCAAATTGATTCGTGGATTCAGGTGACGGAGAAGATTGCGGATCTTTGCTCTCTAAAAATTGATATGGGGCAGCTTCTTTTCCCTAAATATGAAGCGGAGCCTGAGATTCTCGAACTTTATGAAAAAAATAAGGATCAGCTAATTTGTGAATAA", "aa": "MAERVQASCLVSNIYIYPKPEDKPTQELAMAIKDNLKLYDPQHRVLTTENHLMTEEEIRKICLENGYSEAQIDSWIQVTEKIADLCSLKIDMGQLLFPKYEAEPEILELYEKNKDQLICE", "aa_hexdigest": "66c6ec5d79bee800e5f9992a53809526", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13991.91479999999, "isoelectric_point": 4.654852104187013 }, "id": "NKOCPLAKPK_254", "locus": "GCA003260355_00213" }, { "type": "cds", "sequence": "contig_1", "start": 105703, "stop": 106173, "strand": "-", "frame": 1, "gene": "recA", "product": "recombinase RecA", "db_xrefs": [ "GO:0003677", "GO:0003697", "GO:0005524", "GO:0006281", "GO:0008094", "KEGG:K03553", "SO:0001217", "UniRef:UniRef50_A0A7W4E3A7", "UniRef:UniRef90_A0A7W4E3A7" ], "nt": "ATGTTTGGGAATCCAGAGACGACCACAGGAGGAAATGCACTCAAGTTCTTTGCCTCTCAGAGAATAGAAATCAGAAAAGGGGACAAAATCATGGAGGACAAAGAGCAAATCGGATATTTCGCCAAAATTAAAGTCGCTAAAAATAAAATCTTTGCTCCGTTCAAAACTGCTGAATTACCTATAAAATGGAGTATGGGATACGACAAAACAACAGATATAATTGAAGCAGCATTAATTCTAAAGCTTATCTCCAAAGCAGGAGCTTTCTATACCGTAGGAAATGAAAAACTCCAAGGAAAAGAAAAACTCGCTAAATACCTTGAAGGAGAAGAGAAAATCCGCAACAACCTAGAAAAAGAAATCCAGTCAAAAATCAAAGACATGAGACTGGGGAAAAAGGTCCTCGATGATGAAGCTTTAAGCAAAATGGAAGCGATCATAGAAGACGAAAGTCAATCCATCGAAGAATAA", "aa": "MFGNPETTTGGNALKFFASQRIEIRKGDKIMEDKEQIGYFAKIKVAKNKIFAPFKTAELPIKWSMGYDKTTDIIEAALILKLISKAGAFYTVGNEKLQGKEKLAKYLEGEEKIRNNLEKEIQSKIKDMRLGKKVLDDEALSKMEAIIEDESQSIEE", "aa_hexdigest": "bdfc5e31fa3b35b61091c6d5c21a8404", "start_type": "ATG", "rbs_motif": null, "genes": [ "recA" ], "truncated": "3-prime", "pseudogene": { "start": 105703, "stop": 106473, "observations": { "mutation": [ 106179, 106371 ], "stop": [ 106179, 106371 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A7W4E3A7", "query_cov": 1.0, "subject_cov": 0.43820224719101125, "identity": 0.885, "score": 270.0, "evalue": 2.2e-88, "gene_start": 1, "gene_end": 156, "reference_start": 201, "reference_end": 356, "reference_sequence": "MSNVNAQALKDAFMNIEKQFGKGAIMKMGDNANVGLVNTAHSGSYVLDLILGGGYPEGRVIEIYGPESSGKTTIALHAIAEIQKRGEVAAFIDAEHALDPHYAKVLGVNTDELILSQPDYGEQALQIAEELAKTGAVKLIVIDSVAALVPRAEVEGDMGDSYMGLQARMMSQGLRKLTSVLAKTGTMCIFINQIRMKIGIMFGNPETTTGGNALKFFASQRIEIRKGDKIMEDKEQVGYFAKIKIAKNKIFAPFKTAELPIKWQMGYDRTTDIIEAALVLKLISKAGAFYTVGSEKVQGKEKLAKYLDSEEKIRSNLEKEIQAKIKDMRMGKKVLDDEALKSMEAIIEDEISEVEE" }, "paralog": false, "identity": 0.91015625, "score": 457.0, "evalue": 2.08e-164, "description": "Internal stop codon at 106179, 106371. Nonsense mutation around 106179, 106371.", "psc": { "uniref90_id": "UniRef90_A0A7W4E3A7", "gene": "recA", "product": "recombinase RecA", "uniref50_id": "UniRef50_A0A7W4E3A7", "kegg_orthology_id": "K03553", "go_ids": [ "GO:0003677", "GO:0003697", "GO:0005524", "GO:0006281", "GO:0008094" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A7W4E3A7", "UniRef:UniRef50_A0A7W4E3A7", "KEGG:K03553", "GO:0003677", "GO:0003697", "GO:0005524", "GO:0006281", "GO:0008094" ] }, "pscc": { "uniref50_id": "UniRef50_A0A7W4E3A7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7W4E3A7" ], "product": "Protein RecA" } }, "id": "NKOCPLAKPK_255", "locus": "GCA003260355_00214" }, { "type": "cds", "sequence": "contig_1", "start": 106177, "stop": 106359, "strand": "-", "frame": 1, "gene": "recA", "product": "Protein RecA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A920RYF0" ], "nt": "ATGATCGTAATTGATTCGGTAGCTGCCCTTGTTCCAAGAGCCGAAGTGGAAGGCGATATGGGAGATTCTTACATGGGACTTCAGGCGAGAATGATGTCACAGGGACTTAGAAAGCTGACTTCCGTCCTCGCAAAAACTGGCACAATGTGTATCTTCATCAACCAAATCAGAATGAAAATCTGA", "aa": "MIVIDSVAALVPRAEVEGDMGDSYMGLQARMMSQGLRKLTSVLAKTGTMCIFINQIRMKI", "aa_hexdigest": "db7e434a4be691946da2724ed22876b4", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A920RYF0", "query_cov": 0.9333333333333333, "subject_cov": 0.9655172413793104, "identity": 0.607, "score": 67.8, "evalue": 6.85e-14, "valid": false, "product": "Protein RecA", "uniref50_id": "UniRef50_A0A920RYF0" }, "pscc": { "uniref50_id": "UniRef50_A0A920RYF0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A920RYF0" ], "product": "Protein RecA" }, "genes": [ "recA" ], "id": "NKOCPLAKPK_256", "locus": "GCA003260355_00215" }, { "type": "cds", "sequence": "contig_1", "start": 106369, "stop": 106596, "strand": "-", "frame": 1, "gene": null, "product": "ATPase domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0023B7D3D0" ], "nt": "GTGATTGAAATCTACGGTCCTGAATCTTCAGGTAAGACGACGATTGCACTCCATGCAATTGCAGAGATGCAAAAAAGAGGAGAAACAGCAGCTTTCATTGATGCCGAACACGCATTAGACCCACATTATGCTAAAAAATTAGGAGTCAATACTGAAGAACTCATTCTCTCTCAACCAGACTACGGAGAACAAGCCCTTCAAATCGCAGAAGAACTCGCAAAAACTTGA", "aa": "MIEIYGPESSGKTTIALHAIAEMQKRGETAAFIDAEHALDPHYAKKLGVNTEELILSQPDYGEQALQIAEELAKT", "aa_hexdigest": "8f1905434af67e2705beaa9328c4295a", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0023B7D3D0", "query_cov": 0.8666666666666667, "subject_cov": 0.8441558441558441, "identity": 0.677, "score": 92.8, "evalue": 2.53e-23, "valid": false, "product": "ATPase domain-containing protein", "uniref50_id": "UniRef50_UPI0023B7D3D0" }, "pscc": { "uniref50_id": "UniRef50_UPI0023B7D3D0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0023B7D3D0" ], "product": "ATPase domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_257", "locus": "GCA003260355_00216" }, { "type": "cds", "sequence": "contig_1", "start": 106852, "stop": 107061, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTGTTTAAGGAAAAAAAACAAAAAATCAAGAGAATTTTTATAATTTTTCATTGGGATTATTTTTTTGTCAAAAAAAAGTTTGATTTCGTCAAAAAAGATTTGACATTGTCAAAGAGATTCGTAGACTATGTTTATATCTTTATGTTGTTATCTGGAGTAAATGATCAACAAACATATTATTGGACTAAAAATTACCACAACAAATGA", "aa": "MVFKEKKQKIKRIFIIFHWDYFFVKKKFDFVKKDLTLSKRFVDYVYIFMLLSGVNDQQTYYWTKNYHNK", "aa_hexdigest": "6ce79902bb4bfcbde679e9cd3b2c13bf", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8788.288799999998, "isoelectric_point": 9.860519981384279 }, "id": "NKOCPLAKPK_258", "locus": "GCA003260355_00217" }, { "type": "cds", "sequence": "contig_1", "start": 107015, "stop": 107443, "strand": "+", "frame": 2, "gene": null, "product": "Integron-associated effector binding protein domain-containing protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBF49F8", "UniRef:UniRef100_A0A328EHG4", "UniRef:UniRef50_A0A328EHG4", "UniRef:UniRef90_A0A328EHG4" ], "nt": "ATGATCAACAAACATATTATTGGACTAAAAATTACCACAACAAATGAGAATAATCAAGCACAGGAAGATATTAATCAGCTCCGAAAAAGCTTTTATGATCAGGCAAATACTGAACTTCAAACCTTTAAAAAAGAGCCTTATACCTATGTTGTATATACTAATTATCAGGGAGATTTTCAGCAAGGTAACTATGATTGTTATCTAGGAATAGCCAGTGAAAGTACCCTTACAGGATTTGCATCTTGTGATATCAAGCTTGAGAAGTATCAGATTTTTGATTTTCCTTATGATAATCCTCAAGATACGCTTGAGGCACGAAAAACGATTTGGGCGGATCAATCTCTCAAAAGAGCTTATCTAGCAGACCTTGAAATCTACGACTTTGAGCATAATCGCCTACAAATTATTATCAGCACCATTGAAGACTAA", "aa": "MINKHIIGLKITTTNENNQAQEDINQLRKSFYDQANTELQTFKKEPYTYVVYTNYQGDFQQGNYDCYLGIASESTLTGFASCDIKLEKYQIFDFPYDNPQDTLEARKTIWADQSLKRAYLADLEIYDFEHNRLQIIISTIED", "aa_hexdigest": "200006a829d9f527ddc2aca7ab44d540", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000DBF49F8", "uniref100_id": "UniRef100_A0A328EHG4", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBF49F8", "UniRef:UniRef100_A0A328EHG4" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EHG4", "uniref90_id": "UniRef90_A0A328EHG4" }, "psc": { "uniref90_id": "UniRef90_A0A328EHG4", "product": "Integron-associated effector binding protein domain-containing protein", "uniref50_id": "UniRef50_A0A328EHG4" }, "pscc": { "uniref50_id": "UniRef50_A0A328EHG4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EHG4" ], "product": "Integron-associated effector binding protein domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_259", "locus": "GCA003260355_00218" }, { "type": "cds", "sequence": "contig_1", "start": 107563, "stop": 107757, "strand": "+", "frame": 1, "gene": "tfoX", "product": "TfoX C-terminal domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1F4ZVH6", "UniRef:UniRef90_A0A328EFZ0" ], "nt": "TTGGCACAAGATCCTTATGAAGTCTTTCATCAGTTCAAAATCAAAGTTGATCCTAGTCTTTGTCGCTGTGCTTTGGCGAGCATTATTGGAGCATACAAGGGAATAAAACGAAACCTGATTCGTGAGGACGCGATTAAAACTTATGAACAGCGTTATCCTGATGATGAACGAAAAACTGACCGAAAAGGTTGTTAA", "aa": "MAQDPYEVFHQFKIKVDPSLCRCALASIIGAYKGIKRNLIREDAIKTYEQRYPDDERKTDRKGC", "aa_hexdigest": "7e772ecfd69544a2bc379a0dacf13f7d", "start_type": "TTG", "rbs_motif": null, "genes": [ "tfoX" ], "truncated": "5-prime", "pseudogene": { "start": 107470, "stop": 107757, "observations": { "mutation": [ 107506, 107539 ], "stop": [ 107506, 107539 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EFZ0", "query_cov": 1.0, "subject_cov": 0.6736842105263158, "identity": 0.875, "score": 122.0, "evalue": 6.91e-35, "gene_start": 1, "gene_end": 64, "reference_start": 32, "reference_end": 95, "reference_sequence": "MPRSPLCKLPNIGPRIADNLIKIGIMTPEEFLAQDPYEVFHQLKMKVDPSLCRCALASIIGAYKGIKRNLIREEAVKTYEQRYPDDERRTSRRGC" }, "paralog": false, "identity": 0.8842105263157894, "score": 176.0, "evalue": 6.54e-59, "description": "Internal stop codon at 107506, 107539. Nonsense mutation around 107506, 107539.", "psc": { "uniref90_id": "UniRef90_A0A328EFZ0", "product": "TfoX C-terminal domain-containing protein", "uniref50_id": "UniRef50_A0A1F4ZVH6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EFZ0", "UniRef:UniRef50_A0A1F4ZVH6" ] }, "pscc": { "uniref50_id": "UniRef50_A0A1F4ZVH6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1F4ZVH6" ], "product": "TfoX C-terminal domain-containing protein" } }, "id": "NKOCPLAKPK_260", "locus": "GCA003260355_00219" }, { "type": "cds", "sequence": "contig_1", "start": 107723, "stop": 107896, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATGAACGAAAAACTGACCGAAAAGGTTGTTAATCTAACTTTTATCTCTTTTTACTATAATAGTATGGCAACGAATAAACTTGATGATTTACAAAGAAGATTGATCGCATTTTTTAAGCAAAATACTGAACAGGAAGGACTTACTCTCAGGGAAATTGCAAATCAAGTTTGA", "aa": "MMNEKLTEKVVNLTFISFYYNSMATNKLDDLQRRLIAFFKQNTEQEGLTLREIANQV", "aa_hexdigest": "65ed6a6c548ce312933054cbb6ed8e68", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6759.696499999998, "isoelectric_point": 6.133404731750488 }, "id": "NKOCPLAKPK_261", "locus": "GCA003260355_00220" }, { "type": "cds", "sequence": "contig_1", "start": 107984, "stop": 108409, "strand": "+", "frame": 2, "gene": null, "product": "Peptidase S24/S26A/S26B/S26C domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A351Y7H7" ], "nt": "TTGAGAGAAGATGTAGAAAATAAGTTGGATGATATTGTTCAGCTTCCGATCTATGGTTTCGCACAGTGTGGAAATAAAGGAAAGCAGATCATTGATGAATATTCTCAAGAAAAAATGCCGGTAACAATCGCATTTATTGGGACAAGTGATATTAAAAATTGTTTTTTTGTCAGGGCAAAAGGGAATAGTATGGAGCCGAAAATCCAGGATGGCGACCTCGTTCTTATTCGCCAACAACCAAGTTATGATGAATCAGATTTCGTTTTTGTTTTGCATAATCAGCTTCCTAAGCTCAAAAAAATTATCAACCAAAATGGGCATTATATGCTAGAATCAATTAATCGTTTTTTTGATAAGGTGGAAATTGATCCGTATGATGAGACGAAAGTGATCGGAGTTGTGAAAAAGGTTATTAAGAGTATGTAG", "aa": "MREDVENKLDDIVQLPIYGFAQCGNKGKQIIDEYSQEKMPVTIAFIGTSDIKNCFFVRAKGNSMEPKIQDGDLVLIRQQPSYDESDFVFVLHNQLPKLKKIINQNGHYMLESINRFFDKVEIDPYDETKVIGVVKKVIKSM", "aa_hexdigest": "8e82e2321d890c6d74c41105f50af13c", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A351Y7H7", "query_cov": 1.0, "subject_cov": 0.9929577464788732, "identity": 0.716, "score": 222.0, "evalue": 1.17e-72, "valid": false, "product": "Peptidase S24/S26A/S26B/S26C domain-containing protein", "uniref50_id": "UniRef50_A0A351Y7H7" }, "pscc": { "uniref50_id": "UniRef50_A0A351Y7H7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A351Y7H7" ], "product": "Peptidase S24/S26A/S26B/S26C domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_262", "locus": "GCA003260355_00221" }, { "type": "cds", "sequence": "contig_1", "start": 108542, "stop": 108763, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTAGTTATCGATCTTTTGGTGGATAAGGTCCCTTACTTCCTGATATTTTTCCAAATTTTTAAATACAATTGGTTTATCGTTCCCCATTTGTATTTCAATTCCTCCAAATCAGAGGAAATGTTTTATTTCTACAGAATTGATTTTTTTGTATTTTATATCTTTTTGTTCCCTGAAGAGGAGGCCTTTTTCAATTACTACACTATCATCTTTAATGATTAA", "aa": "MLVIDLLVDKVPYFLIFFQIFKYNWFIVPHLYFNSSKSEEMFYFYRIDFFVFYIFLFPEEEAFFNYYTIIFND", "aa_hexdigest": "3ffa8c87a72665e570637c7cc7f5a1b1", "start_type": "TTG", "rbs_motif": "TTTTT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9299.705399999993, "isoelectric_point": 4.480697441101075 }, "id": "NKOCPLAKPK_263", "locus": "GCA003260355_00222" }, { "type": "cds", "sequence": "contig_1", "start": 108794, "stop": 108964, "strand": "-", "frame": 3, "gene": null, "product": "YdbS-like PH domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EJ70", "UniRef:UniRef90_A0A328EJ70" ], "nt": "ATGGGGAGGATTTATGGTAAAAATTCACTAGTTAAGTGGGGATGGCTCATCTCTACAGGACTATGGGCACTTTTTGGAATCATTCATACTCTTTCTCTGACGGGAGATATAGGTATTGGGTTAGCATTTCAGGGAGCATTTATTTGGTGGCTGCTTTTGGTTATTTGCTGA", "aa": "MGRIYGKNSLVKWGWLISTGLWALFGIIHTLSLTGDIGIGLAFQGAFIWWLLLVIC", "aa_hexdigest": "317dcfb729f12c94efe6cd84dfad41a7", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 108545, "stop": 108964, "observations": { "mutation": [ 108655, 108796 ], "stop": [ 108655, 108796 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EJ70", "query_cov": 1.0, "subject_cov": 0.4028776978417266, "identity": 1.0, "score": 119.0, "evalue": 2.09e-33, "gene_start": 1, "gene_end": 56, "reference_start": 1, "reference_end": 56, "reference_sequence": "MGRIYGKNSLVKWGWLISTGLWALFGIIHTLSLTGDIGIGLAFQGAFIWWLLLVICGIKTLQVFAQKLIIKDDSVVIEKGLLFREQKDIKYKKINSVEIKHFLGFGGIEIQMGNDKPIVFKNLEKYQEVRDLIHQKIDN" }, "paralog": false, "identity": 0.9856115107913669, "score": 276.0, "evalue": 7e-98, "description": "Internal stop codon at 108655, 108796. Nonsense mutation around 108655, 108796.", "psc": { "uniref90_id": "UniRef90_A0A328EJ70", "product": "YdbS-like PH domain-containing protein", "uniref50_id": "UniRef50_A0A328EJ70", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EJ70", "UniRef:UniRef50_A0A328EJ70" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EJ70", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EJ70" ], "product": "YdbS-like PH domain-containing protein" } }, "id": "NKOCPLAKPK_264", "locus": "GCA003260355_00223" }, { "type": "cds", "sequence": "contig_1", "start": 109768, "stop": 111069, "strand": "-", "frame": 1, "gene": null, "product": "DNA primase", "db_xrefs": [ "EC:2.7.7.101", "KEGG:K02316", "SO:0001217", "UniRef:UniRef50_A0A288DIY8", "UniRef:UniRef90_A0A660MV72" ], "nt": "GTGAGTAATCCAATCATAGATGATATTCTCCATTCCACAGATATTGTAGATATCGTCGGAAAATATGTTCCACTAAAAAAAGCTGGAAGTAATTTTTCTGGATGTTGCCCATTCCACAACGAAAAGACTCCTTCATTTATGGTTTCGCCAACAAAACAGATTTTCAAATGCTTTGGCTGCGGAAAAGGAGGGAACGTGCTAACCTTTATGCAAGAAATAGAAAAAATTGACTTCCGAGACGCGGTCAAAGAGCTCGCAAAAATGCAGCATATCGACCTTGAAAAATACGATACATCTCTGAAGAAAAACGCTGCAGAAAGCGACGAAAAAGGGAAGCTCAAAAGAATCCATACCCTTGCTCAAAATTTTTTCAGAGAAAACCTCCAAAATCATCCAGAAGCTCAGCAATATTTACAAGAAGTGAGAAAACTTTCCCCAGAATTGATTGAACGTTTTGGAATAGGATACGCACCAGAAAGCTATTATGAGTTAGTCCAACGCCTGAGGACAAAGGGCTTCAACGACAACGATATTTTAGAGGCATCTTTGGCAAAAAGAAACGCTAATGGTGAAATTTATGCGTTTTTTAGAAACAGAATTACCTTTCCAATCGCAGATACCATGGGAAATATCGTAGGTTTTTCTGCCAGAGTGCTAAATCCTGAAGATAAACCCAAATACCTCAACAGTGCAGAACACAAAGCCTTTGAAAAATCAAAGATCCTCTATGGATTAAACCTTGCTAAAAATGCTATTAGAGAACAGGAAAAGCTGATCATTGTGGAAGGACAGATGGACGTTTTAGGACTTGCAAAACTTGATATGCCAATCGGAGTTGCAACTTCCGGGACTGCACTCACCGAAGACCACGTAAAAACCCTGAAAAGATATACTGAAAATATCTACCTCCTCTTCGATAACGATAGTGCGGGACAGCAAGCTACCAAAAGAGCACTCAAACTCTTTTATCAGCAAAACCTTTTCCCAAAAATTATAAAACTCCCTACAGAACAAAAAGACGTTGATGACCTTGCTAATACACCAGAAGGAAAGGAGACATTTAACCACTGCTTAAATTCCGCTCAAGACGGCTTCATCACCGTATACCAAGATCTTCGTTCACAGCTAGACATGAGCTCTCCCATCGACAAACAAAAGCTGATCAATACGCTCTTCGAAATGATTCTCTCAGTTAATAGCCTCACTATCCAGGAACATTATAAAAGCCTTTTAGCAGAAGAACTGGGATTTGCCTTTGAAATCCTTGACGCCCAATTCAAAAGATACAAAAATACTGACTGA", "aa": "MSNPIIDDILHSTDIVDIVGKYVPLKKAGSNFSGCCPFHNEKTPSFMVSPTKQIFKCFGCGKGGNVLTFMQEIEKIDFRDAVKELAKMQHIDLEKYDTSLKKNAAESDEKGKLKRIHTLAQNFFRENLQNHPEAQQYLQEVRKLSPELIERFGIGYAPESYYELVQRLRTKGFNDNDILEASLAKRNANGEIYAFFRNRITFPIADTMGNIVGFSARVLNPEDKPKYLNSAEHKAFEKSKILYGLNLAKNAIREQEKLIIVEGQMDVLGLAKLDMPIGVATSGTALTEDHVKTLKRYTENIYLLFDNDSAGQQATKRALKLFYQQNLFPKIIKLPTEQKDVDDLANTPEGKETFNHCLNSAQDGFITVYQDLRSQLDMSSPIDKQKLINTLFEMILSVNSLTIQEHYKSLLAEELGFAFEILDAQFKRYKNTD", "aa_hexdigest": "720655e59db8bd4690404a239270aa0f", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 109465, "stop": 111069, "observations": { "mutation": [ 109560, 109650, 109770 ], "stop": [ 109560, 109650, 109770 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A660MV72", "query_cov": 1.0, "subject_cov": 0.7543554006968641, "identity": 1.0, "score": 857.0, "evalue": 8.15e-312, "gene_start": 1, "gene_end": 433, "reference_start": 1, "reference_end": 433, "reference_sequence": "MSNPIIDDILHSTDIVDIVGKYVPLKKAGSNFSGCCPFHNEKTPSFMVSPTKQIFKCFGCGKGGNVLTFMQEIEKIDFRDAVKELAKMQHIDLEKYDTSLKKNAAESDEKGKLKRIHTLAQNFFRENLQNHPEAQQYLQEVRKLSPELIERFGIGYAPESYYELVQRLRTKGFNDNDILEASLAKRNANGEIYAFFRNRITFPIADTMGNIVGFSARVLNPEDKPKYLNSAEHKAFEKSKILYGLNLAKNAIREQEKLIIVEGQMDVLGLAKLDMPIGVATSGTALTEDHVKTLKRYTENIYLLFDNDSAGQQATKRALKLFYQQNLFPKIIKLPTEQKDVDDLANTPEGKETFNHCLNSAQDGFITVYQDLRSQLDMSSPIDKQKLINTLFEMILSVNSLTIQEHYKSLLAEELGFAFEILDAQFKRYKNTDGKFYATQSSQKNSQEHEHYQLERELLFVALFYQEHLNTYLGNNAASQGLLYFIQLLTKADPESLLNQALHGEIPSEQKQKIEELTLRRDNQLEPLTTPEKKYQMILQIILPTLQKMLQFILKNPKIPANLKAELLAERKNL" }, "paralog": false, "identity": 0.9925093632958801, "score": 1050.0, "evalue": 0.0, "description": "Internal stop codon at 109560, 109650, 109770. Nonsense mutation around 109560, 109650, 109770.", "psc": { "uniref90_id": "UniRef90_A0A660MV72", "product": "DNA primase", "ec_ids": [ "2.7.7.101" ], "uniref50_id": "UniRef50_A0A288DIY8", "kegg_orthology_id": "K02316", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A660MV72", "EC:2.7.7.101", "UniRef:UniRef50_A0A288DIY8", "KEGG:K02316" ] }, "pscc": { "uniref50_id": "UniRef50_A0A288DIY8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A288DIY8" ], "product": "DNA primase" } }, "id": "NKOCPLAKPK_265", "locus": "GCA003260355_00224" }, { "type": "cds", "sequence": "contig_1", "start": 111239, "stop": 111679, "strand": "+", "frame": 2, "gene": null, "product": "Signal peptidase (SPase) II", "db_xrefs": [ "EC:3.4.23.36", "KEGG:K03101", "SO:0001217", "UniParc:UPI000DBFBE4B", "UniRef:UniRef100_A0A328EJQ8", "UniRef:UniRef50_A0A7W4E339", "UniRef:UniRef90_A0A328EJQ8" ], "nt": "ATGAAGTATCGAAAAATGACTAGTATTGGAGTTTTGCTGCTTGGTTTGGATCTCTTGACGAAATGGCTTTTTTATGATTTGCAACTTGGGGAGGAATTGCCTTTTCTTCATCCGACGCTAAATACCGGGATTTCTCGAGGGATCAAAATCTATCTTCCTTTTGTTATTTTGATTTCATTCGTAGGGATTGGGCTTTTCTTTTGGTTATGGAGACAAAAAAGGTTTGGAAATCTGATTTTTCTTCTTTTTCTTGCTGGAACGCTCGGAAATCTTGTTGATAGAGTGTTTTTGGCTGGAGTAAGGGATTTTATTAGCATTGGAAGCTTCCCTGTTTTCAATCTCGCGGACTGTTTCCTTACGCTAGCTGTAGGAATTTTGTGTCGAAATGAGATTTTCCCCTTGCAATTAAAAAAGAAAACCGTATCTTCGGGTAAAGTTTAA", "aa": "MKYRKMTSIGVLLLGLDLLTKWLFYDLQLGEELPFLHPTLNTGISRGIKIYLPFVILISFVGIGLFFWLWRQKRFGNLIFLLFLAGTLGNLVDRVFLAGVRDFISIGSFPVFNLADCFLTLAVGILCRNEIFPLQLKKKTVSSGKV", "aa_hexdigest": "5debdb65fda1699fbb619c86fbcb862a", "start_type": "ATG", "rbs_motif": "TTTTT", "ups": { "uniparc_id": "UPI000DBFBE4B", "uniref100_id": "UniRef100_A0A328EJQ8", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBFBE4B", "UniRef:UniRef100_A0A328EJQ8" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EJQ8", "uniref90_id": "UniRef90_A0A328EJQ8" }, "psc": { "uniref90_id": "UniRef90_A0A328EJQ8", "product": "Signal peptidase (SPase) II", "ec_ids": [ "3.4.23.36" ], "uniref50_id": "UniRef50_A0A7W4E339", "kegg_orthology_id": "K03101" }, "pscc": { "uniref50_id": "UniRef50_A0A7W4E339", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7W4E339" ], "product": "Lipoprotein signal peptidase" }, "genes": [], "id": "NKOCPLAKPK_266", "locus": "GCA003260355_00225" }, { "type": "cds", "sequence": "contig_1", "start": 111708, "stop": 112019, "strand": "+", "frame": 3, "gene": null, "product": "DUF3127 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBFF151", "UniRef:UniRef100_A0A660MW13", "UniRef:UniRef50_A0A288DGN7", "UniRef:UniRef90_A0A660MW13" ], "nt": "ATGCAGTATCAAGGATTTGTGAAGTTTATCGGTCCTAAGGAGACGATTGGTGACGGAGTTGAAAAACAGACGATCGTTTTGGAGGAGAATACAGATAGAGAGTTTAAGGGCTCTATTGCGGTAGATTTCTTTAGAGATAAGATCAATCTTTTGTCAGATGTTAATACTGGAGATTTGATTACTGTTCACTTGAATGCAAGAGCTAATGAATCTAGAAATCAACCAGGAAGATGGTTCAATAGCATCACTTGCTGGAGAGTAGAAAAGGCAGGAGCGACTCCAAGTGCTTCTTCTGATGATCTTCCTTTCTAA", "aa": "MQYQGFVKFIGPKETIGDGVEKQTIVLEENTDREFKGSIAVDFFRDKINLLSDVNTGDLITVHLNARANESRNQPGRWFNSITCWRVEKAGATPSASSDDLPF", "aa_hexdigest": "af99b47a2d5e2abd5846bf8c571d4806", "start_type": "ATG", "rbs_motif": "TTATT", "ups": { "uniparc_id": "UPI000DBFF151", "uniref100_id": "UniRef100_A0A660MW13", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBFF151", "UniRef:UniRef100_A0A660MW13" ] }, "ips": { "uniref100_id": "UniRef100_A0A660MW13", "uniref90_id": "UniRef90_A0A660MW13" }, "psc": { "uniref90_id": "UniRef90_A0A660MW13", "product": "DUF3127 domain-containing protein", "uniref50_id": "UniRef50_A0A288DGN7" }, "pscc": { "uniref50_id": "UniRef50_A0A288DGN7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A288DGN7" ], "product": "DUF3127 domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_267", "locus": "GCA003260355_00226" }, { "type": "cds", "sequence": "contig_1", "start": 112172, "stop": 112654, "strand": "-", "frame": 3, "gene": null, "product": "N-acetyltransferase domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EJ79", "UniRef:UniRef90_A0A328EJ79" ], "nt": "ATGACAGAAAGAGTAAAAATTTTGCAAGGGGAAAGGGTTGCACTTGCTCTCATCGAGAAAGAAGATAGTCTACTTCGGTATCGCCATATGAATGACCTAGAGGTGCAGCAGTTTCTAAGCGTCGGCGGATGCCTGCTTTACAAAGAAGCTGAACAAGACTATTATGACCACATAATCAAAAATCCCAATATGAGAGTCTTCGCGATCTGTGTTGAGGGCGAAAAGAATATCGGAACCATTTCCCTTGAAATCAACCGAAAAAACAGGAATGCCGAACTTGGAATCGCAATTTTCGACAAAAACTACTGGTCTCAAGGCTATGGAACAGAAAGTATTCAGCTCCTCTTCAAGTATGCTTTTGAAATCCTCGGACTTCACAAAGTATATTTGAATGTCGTAGCGAATAATGCAAGAGCGGCAAAAGCATATGAAAAAGTAGGGTTCAAAGAAATTGGCAGAAGAAAAGAGCATTGGTATTTCTGA", "aa": "MTERVKILQGERVALALIEKEDSLLRYRHMNDLEVQQFLSVGGCLLYKEAEQDYYDHIIKNPNMRVFAICVEGEKNIGTISLEINRKNRNAELGIAIFDKNYWSQGYGTESIQLLFKYAFEILGLHKVYLNVVANNARAAKAYEKVGFKEIGRRKEHWYF", "aa_hexdigest": "84d9e5be73c2bae1915cace28909a544", "start_type": "ATG", "rbs_motif": "TTCTT", "psc": { "uniref90_id": "UniRef90_A0A328EJ79", "query_cov": 1.0, "subject_cov": 0.8888888888888888, "identity": 1.0, "score": 324.0, "evalue": 5.25e-112, "valid": true, "product": "N-acetyltransferase domain-containing protein", "uniref50_id": "UniRef50_A0A328EJ79" }, "pscc": { "uniref50_id": "UniRef50_A0A328EJ79", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EJ79" ], "product": "N-acetyltransferase domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_268", "locus": "GCA003260355_00227" }, { "type": "cds", "sequence": "contig_1", "start": 112693, "stop": 113034, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00152.25" ], "nt": "GTGGATGGATTCCCTCGATCAGATGCAAAATTCTACCACCACCAAAATCAAGAAAATCCTGAAGTTGCCGATCGTGCAGATCTCCTTTTCAGAGGCGTAGAAATCGTCACTTTGACGCAGCGTGAGGTAAATTACCAGAGATTGGTAGATCAAATCACCGCACAGGGACAGAATCCAGAACATCCAGGATTGAAGCATTACTTAGACGCCTTTAAATACGGAATGCCTGATGAAGGAGGATTTGGACTCGGAGTTGCGAGGTTTGTCCAGAAACTTATTGGGCTCGAAAATATCAAGGAAGCAGAACTCTTCCCAAGAGATATGCAAAGAGTCACTCCATAA", "aa": "MDGFPRSDAKFYHHQNQENPEVADRADLLFRGVEIVTLTQREVNYQRLVDQITAQGQNPEHPGLKHYLDAFKYGMPDEGGFGLGVARFVQKLIGLENIKEAELFPRDMQRVTP", "aa_hexdigest": "576f6af6b3b5ede619682da0ab24884b", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A0G1KMD6", "query_cov": 0.8938053097345132, "subject_cov": 0.9017857142857143, "identity": 0.505, "score": 101.0, "evalue": 1.33e-25, "valid": false, "product": "Aspartyl-tRNA synthetase", "uniref50_id": "UniRef50_A0A0G1KMD6" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00152.25", "name": "tRNA-synt_2", "length": 111, "aa_cov": 0.9292035398230089, "hmm_cov": 1.0, "evalue": 3.4569537992746566e-25, "score": 85.1409912109375, "start": 3, "stop": 107 } ], "seq_stats": { "molecular_weight": 12956.42999999999, "isoelectric_point": 5.4989110946655275 }, "id": "NKOCPLAKPK_269", "locus": "GCA003260355_00228" }, { "type": "cds", "sequence": "contig_1", "start": 113092, "stop": 113334, "strand": "-", "frame": 1, "gene": null, "product": "Asparagine--tRNA ligase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1V5ZKS4", "UniRef:UniRef90_A0A1V5ZKS4" ], "nt": "ATGCTCGATATGGAAATGGGATTTATTGATAGTTTTGATGATGTGATTGAGATGACAGAGAGATTTGCCAACTTTGTAGTAGAGCAAACCTGGGCTGAAGCTGAAGACTTACTCCTCGCACTCGGTGCAACCAAACCACTCCTCGTAGAACACTATCCTCGCATCAGTGTAGACGCACTACACGAATTGATGTTCAAAGAGACAGGAGAAGATTATCGCCATGAAAGAGATCTTGCACCTTGA", "aa": "MLDMEMGFIDSFDDVIEMTERFANFVVEQTWAEAEDLLLALGATKPLLVEHYPRISVDALHELMFKETGEDYRHERDLAP", "aa_hexdigest": "fec878c69d9c28ea127303a01a186f4f", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 113026, "stop": 113424, "observations": { "mutation": [ 113094, 113391 ], "stop": [ 113094, 113391 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A1V5ZKS4", "query_cov": 1.0, "subject_cov": 0.6060606060606061, "identity": 0.838, "score": 139.0, "evalue": 5.71e-41, "gene_start": 1, "gene_end": 80, "reference_start": 31, "reference_end": 110, "reference_sequence": "MVPVFERVYEIGRAYRAEKSNTSRHLSEILMLDMEMGFIDSFDDIIEMTEKFANFVVKQTWTEAEHLLTALGATKPLLVDQFPRISVDDLHELMLKETGEDYRKEKDLAPSEEKFICEYSAKHWNSDFVIVD" }, "paralog": false, "identity": 0.8636363636363636, "score": 236.0, "evalue": 1.69e-81, "description": "Internal stop codon at 113094, 113391. Nonsense mutation around 113094, 113391.", "psc": { "uniref90_id": "UniRef90_A0A1V5ZKS4", "product": "Asparagine--tRNA ligase", "uniref50_id": "UniRef50_A0A1V5ZKS4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A1V5ZKS4", "UniRef:UniRef50_A0A1V5ZKS4" ] }, "pscc": { "uniref50_id": "UniRef50_A0A1V5ZKS4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1V5ZKS4" ], "product": "Asparagine--tRNA ligase" } }, "id": "NKOCPLAKPK_270", "locus": "GCA003260355_00229" }, { "type": "cds", "sequence": "contig_1", "start": 113389, "stop": 113778, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00152.25" ], "nt": "ATGCAGAAGGGAAAATTTCGCTTTGAACTCCAAAGAGCCTCTGTAAACATCATTAGAAAAGTAGTATATCCAAGCCCTATCGATATTTCCAAAGATGAGATCCATGCTGATGGTGAAACTCTTCTAGAAAACAAAGTGGTAGCTCTCAGACATCCAAGACAGATGAAAATTTTCAAGCTCGCAGCCATTGTAGAAAAAAACATGAGAAAATTCTTCGATGAGAATGGATTTACCCAGATCAATTCGCCAAAGATTATCGGATTCCCTACAGAGGGAGGAGCAGAAGTATTTGAGATAGATTATTTTGGACGTAAAGCTTACCTTGCTCAATCACCTCAGTTTTACAAACAGATTATGGTTCCAATCTTCGAAAGAGTCTACGAAATCTGA", "aa": "MQKGKFRFELQRASVNIIRKVVYPSPIDISKDEIHADGETLLENKVVALRHPRQMKIFKLAAIVEKNMRKFFDENGFTQINSPKIIGFPTEGGAEVFEIDYFGRKAYLAQSPQFYKQIMVPIFERVYEI", "aa_hexdigest": "9b3bdfc2f788c9f754513a89ed6d9612", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00152.25", "name": "tRNA-synt_2", "length": 99, "aa_cov": 0.7054263565891473, "hmm_cov": 0.9595959595959596, "evalue": 6.2986991739012765e-21, "score": 71.14787292480469, "start": 39, "stop": 129 } ], "seq_stats": { "molecular_weight": 15044.33519999999, "isoelectric_point": 9.328978157043458 }, "id": "NKOCPLAKPK_271", "locus": "GCA003260355_00230" }, { "type": "cds", "sequence": "contig_1", "start": 113875, "stop": 114009, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATAAAAATATGATCCTTTCGGCAGACCTCTCAAAACACGTAGGAGAAGTCGTAGAAATCCGTGGATGGTTGCATACCGTCCGTGTTGTATCAGCAAACCTCGCATTTTTAATCATGAGAGATAAATGCTGA", "aa": "MYKNMILSADLSKHVGEVVEIRGWLHTVRVVSANLAFLIMRDKC", "aa_hexdigest": "1bad4da30cd15c7860187b502ce66e49", "start_type": "ATG", "rbs_motif": "TTATT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5044.982599999999, "isoelectric_point": 9.100760078430177 }, "id": "NKOCPLAKPK_272", "locus": "GCA003260355_00231" }, { "type": "cds", "sequence": "contig_1", "start": 114017, "stop": 114145, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MUD4", "UniRef:UniRef90_A0A328EFQ7" ], "nt": "ATGAAGAAAATAAAACGCCTTAAAAATATAAAAAATAAGTCTTTTTTCAAGAGGGAAAATAAGAAATTTTACTTTTTTGCTGGAGGAATGCTGGTATGTATTTCTTTAAAGGATGAAATCCCTTTTTGA", "aa": "MKKIKRLKNIKNKSFFKRENKKFYFFAGGMLVCISLKDEIPF", "aa_hexdigest": "bf2ca616596637347ccfce5e662bc949", "start_type": "ATG", "rbs_motif": "TTTTTA", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 114017, "stop": 114241, "observations": { "mutation": [ 114143, 114164, 114194 ], "stop": [ 114143, 114164, 114194 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EFQ7", "query_cov": 1.0, "subject_cov": 0.5675675675675675, "identity": 1.0, "score": 85.5, "evalue": 4.6e-21, "gene_start": 1, "gene_end": 42, "reference_start": 1, "reference_end": 42, "reference_sequence": "MKKIKRLKNIKNKSFFKRENKKFYFFAGGMLVCISLKDEIPFGSLWFLGGDFCRIFILQGRSMYIKNSPLDLNP" }, "paralog": false, "identity": 0.9594594594594594, "score": 145.0, "evalue": 4.31e-47, "description": "Internal stop codon at 114143, 114164, 114194. Nonsense mutation around 114143, 114164, 114194.", "psc": { "uniref90_id": "UniRef90_A0A328EFQ7", "uniref50_id": "UniRef50_A0A660MUD4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EFQ7", "UniRef:UniRef50_A0A660MUD4" ] }, "pscc": { "uniref50_id": "UniRef50_A0A660MUD4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MUD4" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 5087.168299999999, "isoelectric_point": 10.246363258361814 }, "id": "NKOCPLAKPK_273", "locus": "GCA003260355_00232" }, { "type": "cds", "sequence": "contig_1", "start": 114372, "stop": 114524, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGACCATCGACAAATATAGCAATGAAGGAACAATTAAAAATATCCCAGAATTCACAGAAGGGGTACAGAAAGTAATAGAAAGAGAAAACAAGTCTCAAAGCTATGAAAGGTTCAATACCCTTATAAAAGACATTGAGGTAGAAATTAATTGA", "aa": "MTIDKYSNEGTIKNIPEFTEGVQKVIERENKSQSYERFNTLIKDIEVEIN", "aa_hexdigest": "4382fb022c183df76772d9aa67eebc84", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5892.5154999999995, "isoelectric_point": 4.761823081970214 }, "id": "NKOCPLAKPK_274", "locus": "GCA003260355_00233" }, { "type": "cds", "sequence": "contig_1", "start": 114841, "stop": 115107, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTCCAAGGCCTCAATCTGATTCTGAAATTTCAATTTTTTTCGTTTCTGAGTTTGTCTTTCGTTTTAAAATGTTTGGTAATGTCGGATCTCCTACTCACTCTTGTGCCATCATCGCCATAAGGAGTCATCTGGGAAGCTTGTATTTATCTTCTGCAGCATCTGTTATTGATTTTCGTCTTAGGCATCTTAGGATTTTTTCTGCTTTGGATAATTTACACTTTATCAAGTCTTCTTGTGATAGTTTTGGAAAATTTATAAAGTGA", "aa": "MSPRPQSDSEISIFFVSEFVFRFKMFGNVGSPTHSCAIIAIRSHLGSLYLSSAASVIDFRLRHLRIFSALDNLHFIKSSCDSFGKFIK", "aa_hexdigest": "4bd7c763db35437b4741d2133a4542d5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9918.37729999999, "isoelectric_point": 9.586464881896973 }, "id": "NKOCPLAKPK_275", "locus": "GCA003260355_00234" }, { "type": "cds", "sequence": "contig_1", "start": 115798, "stop": 116547, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03477.21" ], "nt": "ATGAGCATCCAAAAAGTAATCAAGAGGAACGGTTCAGAAGTTCCTTATAATCCTGAGAAGATCCATATTGCGATCCAGAAAGCCAATAAAGAAGTGCCTGAATCAGAACAAGCGAGTGAACTCGTAATTGAAGAGGTGATTAGGGCTTTGGAGGCTTCAAAGAATGGGACGCTTCCGATAGAAGAAATTCAAGATTTTATTGAAATGCAGCTAATCAAGCTGAATAAAGCTGAACTTGCGAAGAAATATATCATCTACCGCTATCAGAGAGCTTTGATTAGAAAGGCAAATACGACCGATGATTCCATTCTCACGCTGATCAGAAATGATAATGTGGAGGTGGGGCAGGAAAACTCTAATAAGGATTCTGCGATCGCTTCTACTCAGAGAGATCTCATTGCAGGAGAGGTTTCCAAGGATCTTGCTCGTAGAGTTTTGCTTCCAGAGGAGATTAGAAATGCACACGATGAGGGGATTCTGCATTTTCATGATATGGATTATTTTGTGCAGCCAATTTTCAACTGCTGTTTGATTGATATCAAGGATATGTTTGAGAATGGAACGGTGATGAATAATAAGCTCGTAGAGACTCCAAAGTCTTTTCAGGTAGCCTGCACGGTGATGACGCAGATTATCGCTTCCGTAGCCTCTTCTCAGTATGGAGGTCAGTCTGTGAATATTAAACATCTTGGGAAATATCTTTTCAATACCAAGAAAAAGTATGAAAGAAAATATAAAGAACAATTCTGA", "aa": "MSIQKVIKRNGSEVPYNPEKIHIAIQKANKEVPESEQASELVIEEVIRALEASKNGTLPIEEIQDFIEMQLIKLNKAELAKKYIIYRYQRALIRKANTTDDSILTLIRNDNVEVGQENSNKDSAIASTQRDLIAGEVSKDLARRVLLPEEIRNAHDEGILHFHDMDYFVQPIFNCCLIDIKDMFENGTVMNNKLVETPKSFQVACTVMTQIIASVASSQYGGQSVNIKHLGKYLFNTKKKYERKYKEQF", "aa_hexdigest": "7c642fc273fc1a91c3a2bfd292a27281", "start_type": "ATG", "rbs_motif": "TTTA", "psc": { "uniref90_id": "UniRef90_A0A6I2R372", "query_cov": 0.9678714859437751, "subject_cov": 0.8456140350877193, "identity": 0.573, "score": 262.0, "evalue": 1.31e-84, "valid": false, "product": "ATP-cone domain-containing protein", "uniref50_id": "UniRef50_A0A6I2R372" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03477.21", "name": "ATP-cone", "length": 87, "aa_cov": 0.3493975903614458, "hmm_cov": 0.9885057471264368, "evalue": 1.4017068989455648e-23, "score": 79.73136901855469, "start": 5, "stop": 91 } ], "seq_stats": { "molecular_weight": 28480.316400000018, "isoelectric_point": 6.548840904235839 }, "id": "NKOCPLAKPK_276", "locus": "GCA003260355_00235" }, { "type": "cds", "sequence": "contig_1", "start": 116587, "stop": 117333, "strand": "+", "frame": 1, "gene": null, "product": "Anaerobic ribonucleoside-triphosphate reductase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_K1SVS8" ], "nt": "ATGAGAATTAAGGACGAACTTGCTTCTGGAGTGCAGACGATTCAATATCAGATCAATACTCTTATGACAACCAATGGGCAGTCGCCTTTTGTAACGCTTTTTCTTGAGCTTGATGAAAAAGATGAGTATTTGGAGGAAACGGCACAGATTATTGAGGAAATCCTAAATCAAAGGATCCAAGGAATCAAAAACGAAGCAGGAGTTTATGTTACGCCAGCCTTTCCAAAGCTCGTGTATGTTTTGGATGAATGTAATGCGCTCAAAGGTGGAAAATATGACTACCTCACTCAGCTTGCGGTTAAATGCTCTGCCAAGAGAATGTATCCTGACTATATTTCAGCCAAGAAGATGAGAGAAATCCAGGAGGGAAATGTCTTTGCTCCAATGGGGTGTCGTGCCTTCCTTTCACCTTGGAAAGATGAAACAGGAAACTATAAGTTTGAAGGGCGTTTTAATCAAGGTGTGGTAACGCTCAATGTTCCTCAGGTTGCAATTCTTGCGAATCACGATGAAAAGAAGTTTTTTGAGCTTTTGGAAGACAGGCTTCAGCTCTGTTTTAAAGCACTCATGTGTAGACACGAAAGGCTCAAAGGAACGCCTTCAGACATTAGTCCTATTCATTGGCAGTATGGAGCGCTTGCAAGGCTCAAGAAAGGAGAAACCATTGATAAATATCTGACTGGTGGGTATTCAACGATTACGCTGGGATATATCGGACTTTATGAGGCTTCAGTTTTGATTACTTGA", "aa": "MRIKDELASGVQTIQYQINTLMTTNGQSPFVTLFLELDEKDEYLEETAQIIEEILNQRIQGIKNEAGVYVTPAFPKLVYVLDECNALKGGKYDYLTQLAVKCSAKRMYPDYISAKKMREIQEGNVFAPMGCRAFLSPWKDETGNYKFEGRFNQGVVTLNVPQVAILANHDEKKFFELLEDRLQLCFKALMCRHERLKGTPSDISPIHWQYGALARLKKGETIDKYLTGGYSTITLGYIGLYEASVLIT", "aa_hexdigest": "54d588328031c394aaee973ba95dcfd3", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002E81082B", "query_cov": 0.9153225806451613, "subject_cov": 0.8284671532846716, "identity": 0.546, "score": 265.0, "evalue": 5.42e-86, "valid": false, "product": "anaerobic ribonucleoside-triphosphate reductase", "uniref50_id": "UniRef50_K1SVS8" }, "pscc": { "uniref50_id": "UniRef50_K1SVS8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_K1SVS8" ], "product": "Anaerobic ribonucleoside-triphosphate reductase" }, "genes": [], "id": "NKOCPLAKPK_277", "locus": "GCA003260355_00236" }, { "type": "cds", "sequence": "contig_1", "start": 117439, "stop": 117936, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01228.26" ], "nt": "TTGTATGGAACACCAGCGGAAAGTCTTTGCTACCGTTTTGCCAAGATTGATAAGAAAAAGTTTGGTGAGATCAAAGATGTGACCGATAAGGGCTACTATACCAACTCTTATCATGTAGATGTGAGAGAGAAGATTGATGCTGTTAGTAAACTCGCTTTTGAATCGCAGTTTCAGAAGATTTCTTCAGGAGGATGTATTTCCTATGTTGAGGTGCCAAATATGCAGAAAAACCTTCCAGCCTTGGAAACGCTCGTTCGCTTCATCTATGACAATATCATGTATGCAGAATTTAATACGAAATCTGACTACTGTCACGAATGTGGATTTGAGGGGGAAATTGTAGTCAATGCCGATATGGAGCGAGAATGTCCGCAATGCCACAATAAAGATCAGAGAAAGATGAATGTGACTCGTAGAACTTGTGGCTATTTAGGAGAGAACTTTCGGAATTTAGGGAAAACTAAGGAGATTTCAAAGAGGGTATTGCATGTAAGTTAG", "aa": "MYGTPAESLCYRFAKIDKKKFGEIKDVTDKGYYTNSYHVDVREKIDAVSKLAFESQFQKISSGGCISYVEVPNMQKNLPALETLVRFIYDNIMYAEFNTKSDYCHECGFEGEIVVNADMERECPQCHNKDQRKMNVTRRTCGYLGENFRNLGKTKEISKRVLHVS", "aa_hexdigest": "b97a562324c20c76442a21fe247aade9", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_R5WCT6", "query_cov": 0.9939393939393939, "subject_cov": 1.0, "identity": 0.823, "score": 287.0, "evalue": 1.13e-97, "valid": false, "product": "Anaerobic ribonucleoside-triphosphate reductase", "uniref50_id": "UniRef50_R5WCT6" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01228.26", "name": "Gly_radical", "length": 106, "aa_cov": 0.6363636363636364, "hmm_cov": 0.9716981132075472, "evalue": 9.463175778468416e-24, "score": 80.47399139404297, "start": 42, "stop": 146 } ], "seq_stats": { "molecular_weight": 19084.532400000015, "isoelectric_point": 8.100210762023927 }, "id": "NKOCPLAKPK_278", "locus": "GCA003260355_00237" }, { "type": "cds", "sequence": "contig_1", "start": 118161, "stop": 118436, "strand": "+", "frame": 3, "gene": null, "product": "DUF192 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EFZ7", "UniRef:UniRef90_A0A328EFZ7" ], "nt": "ATGAAGCCTAGAAAAATCATTGCTATCGGTGTAATAACTTTGTTTTTGTTACTAAGTGCTGGATTGATTCTCGGCTTTTTTTATCAGGATGTTTCTGATAACTATGAGGATATCCTTAGTTTGTCTACAGAAAGTCATAGAGGAGATCAGATTTCTACAAAAGATTTGATGGTTAGGTTTAAAAATGGGAGAGATCAGATTTCTTTTTTGAGATTATCAAACGATCATAGACCCTATTTACTTACCATTCCAGAGGGATTGGATTATCGTTCGTGA", "aa": "MKPRKIIAIGVITLFLLLSAGLILGFFYQDVSDNYEDILSLSTESHRGDQISTKDLMVRFKNGRDQISFLRLSNDHRPYLLTIPEGLDYRS", "aa_hexdigest": "31901c799afd8b5e4c963d5cd6836f88", "start_type": "ATG", "rbs_motif": "TTATT", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 118161, "stop": 118703, "observations": { "mutation": [ 118434, 118524, 118527 ], "stop": [ 118434, 118524, 118527 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EFZ7", "query_cov": 1.0, "subject_cov": 0.5055555555555555, "identity": 0.857, "score": 153.0, "evalue": 1.29e-45, "gene_start": 1, "gene_end": 91, "reference_start": 3, "reference_end": 91, "reference_sequence": "MKLRPLLLLLIGILIVLLSAGLILGFFYQDVSDNYEDILSLSTESHRGDQISTKNLMVRFKNGRDQISFLRLSNDHRPYLLTIPEGLDYRSGGITMIGHFENVVGKDSYLTYDPFPHTLLTGGKKYFNFLLDPSSFYFDSQNTACRRFNDEKRCFFNGKLKERILRVVSDPSNVYVVQID" }, "paralog": false, "identity": 0.9, "score": 324.0, "evalue": 1.45e-115, "description": "Internal stop codon at 118434, 118524, 118527. Nonsense mutation around 118434, 118524, 118527.", "psc": { "uniref90_id": "UniRef90_A0A328EFZ7", "product": "DUF192 domain-containing protein", "uniref50_id": "UniRef50_A0A328EFZ7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EFZ7", "UniRef:UniRef50_A0A328EFZ7" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EFZ7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EFZ7" ], "product": "DUF192 domain-containing protein" } }, "id": "NKOCPLAKPK_279", "locus": "GCA003260355_00238" }, { "type": "cds", "sequence": "contig_1", "start": 118858, "stop": 119037, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAATTTAATTATGGAACTTTTCGTAAGGGACGTGCAATCCCAGAGAAACTAGATTATATGCTAAAACATGCAAAGAAAATATACGTAAATAGTGATGCTCATAGCCTATCCTCATTACAACCATTAAGGAAAGAATGCTATGACTATCTCCAAGAAAAAGGGATATTAATACAATAA", "aa": "MEFNYGTFRKGRAIPEKLDYMLKHAKKIYVNSDAHSLSSLQPLRKECYDYLQEKGILIQ", "aa_hexdigest": "4ffe9771a9616ecb6c52f617ddb9d4d5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6980.0123, "isoelectric_point": 9.131189155578614 }, "id": "NKOCPLAKPK_280", "locus": "GCA003260355_00239" }, { "type": "cds", "sequence": "contig_1", "start": 119984, "stop": 120277, "strand": "+", "frame": 2, "gene": null, "product": "DUF2178 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EJ80", "UniRef:UniRef90_A0A328EJ80" ], "nt": "ATGCTTCTGCTCTTGATCGGCGCAGGAATCAGTCTGTATTATGCCCAAAAAATCAAAAAACAGGAAGGGGCAGTTGAGACTGATGAGATGTTTAAGAAAATTGGGTATACCGCTTTGGCGGCTTCGTTTCAACTTTTGATGGGGATTATTGTGCTCCTTCTTTTGGTTAATGATGTTTCGCCCTTCCTCGAAAAGTTGGGAGCGAGAGGGGTATTGATGATGGTAGCGTTGATCGGGACTGCTTTGGTGCTTTCTTGCTCTTGGTATTATACTCGTCATCCGGATAAAACCTGA", "aa": "MLLLLIGAGISLYYAQKIKKQEGAVETDEMFKKIGYTALAASFQLLMGIIVLLLLVNDVSPFLEKLGARGVLMMVALIGTALVLSCSWYYTRHPDKT", "aa_hexdigest": "7da326731774088065bb7f91ae9db3c7", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 119852, "stop": 120277, "observations": { "mutation": [ 119909 ], "stop": [ 119909 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EJ80", "query_cov": 1.0, "subject_cov": 0.6783216783216783, "identity": 0.948, "score": 177.0, "evalue": 1.36e-55, "gene_start": 1, "gene_end": 97, "reference_start": 45, "reference_end": 141, "reference_sequence": "MKKAKTLRFLGIFLGAWGLGIGADLYRQLPFDPLTPAYGFLIFLMLLLLIGAGISLYYAQKIKKQEGAIETDEMFKKIGYTALAASFQLLMGIIVILLLVHDVSPFLEKLGARGVLMMVALIGTALMLSSSWYYTRHPDKTGL" }, "paralog": false, "identity": 0.9219858156028369, "score": 258.0, "evalue": 4.92e-90, "description": "Internal stop codon at 119909. Nonsense mutation around 119909.", "psc": { "uniref90_id": "UniRef90_A0A328EJ80", "product": "DUF2178 domain-containing protein", "uniref50_id": "UniRef50_A0A328EJ80", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EJ80", "UniRef:UniRef50_A0A328EJ80" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EJ80", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EJ80" ], "product": "DUF2178 domain-containing protein" } }, "id": "NKOCPLAKPK_281", "locus": "GCA003260355_00240" }, { "type": "cds", "sequence": "contig_1", "start": 120302, "stop": 120508, "strand": "+", "frame": 2, "gene": null, "product": "Transcriptional regulator", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DC04F0C", "UniRef:UniRef100_A0A328EFW9", "UniRef:UniRef50_R3TJQ2", "UniRef:UniRef90_A0A328EFW9" ], "nt": "ATGCAGAATAGAATCAAAGAACTAAGGGCAAAGCATCATCTCACTCAGCAACAGCTCGCCGAAAAAGTTGGCGTTAGGAGAGAGACAATCGTTTTCCTTGAAATGGGAAAATATAATCCTTCACTGAAATTAGCATGGCAGATCGCACAGCTTTTTCAATGTCCGATTGAAGAAGTTTTTCTCTTTGATGGAGAACGTGCAGCATAA", "aa": "MQNRIKELRAKHHLTQQQLAEKVGVRRETIVFLEMGKYNPSLKLAWQIAQLFQCPIEEVFLFDGERAA", "aa_hexdigest": "9ca1e5cb9d88d49cbacd4f79f0b1896a", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000DC04F0C", "uniref100_id": "UniRef100_A0A328EFW9", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DC04F0C", "UniRef:UniRef100_A0A328EFW9" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EFW9", "uniref90_id": "UniRef90_A0A328EFW9" }, "psc": { "uniref90_id": "UniRef90_A0A328EFW9", "product": "Transcriptional regulator", "uniref50_id": "UniRef50_R3TJQ2" }, "pscc": { "uniref50_id": "UniRef50_R3TJQ2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_R3TJQ2" ], "product": "HTH cro/C1-type domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_282", "locus": "GCA003260355_00241" }, { "type": "cds", "sequence": "contig_1", "start": 120865, "stop": 121119, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCTTTCCTATTCAGGTTTCCTCACAAATGAAAAAACTGCGAGTCTCGATGCTCTCCAAACTTTAGGTAATATAAGAGAGAAGATTGAGCTTTTGGAAAAGGTCTTTGAACAGCAAGCGACCCCAAAGATTGCGAATCTGCTTCTTCAGGCTTATGTGCTTGATAATCAGTTTGAGAAGGCAAAAAAATTTTATAGTTCATTAGCTTCTGATTTTCAAAATACCTTGCCTAAGGAAATGGCGTTTACTATCTGA", "aa": "MLSYSGFLTNEKTASLDALQTLGNIREKIELLEKVFEQQATPKIANLLLQAYVLDNQFEKAKKFYSSLASDFQNTLPKEMAFTI", "aa_hexdigest": "80e09063ab8447ffbfea54006217bace", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9550.866799999996, "isoelectric_point": 5.311001396179199 }, "id": "NKOCPLAKPK_283", "locus": "GCA003260355_00242" }, { "type": "cds", "sequence": "contig_1", "start": 121363, "stop": 121746, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13428.12", "PFAM:PF14559.12", "PFAM:PF07719.23", "PFAM:PF13174.12" ], "nt": "GTGCCTGCTTATTATCAAGATGGATTGATTGCACATCAGCTTATGAACCAAGGATTCTTCGCTCTTGCCAAGAAAATTGTTCTTCCGATTGTTAATGAACATCCAGACTATATTCTGCCTTATCAGATTCTTGCCAATACCGATTTTTCTATGGGGAAACGAGCTTCTGCAGTGAGTTATTTTTCTCAGCTTCTCAAGCTTGATCCGCAGGAGAAGAATCTCTATCTTTATATGTTGGGAGTATGTTATTATCAGATTGGGAAGTATAGTGATGCTGTGCTTTATCTTGCCCAGATCTCTGATTCAAGGATTCTTCTGGACTCTGATAGGTATTTGATTCTGAGTTATCTTGCTCTTGGAGAAAAGGATAGAGTTATCGCTTGA", "aa": "MPAYYQDGLIAHQLMNQGFFALAKKIVLPIVNEHPDYILPYQILANTDFSMGKRASAVSYFSQLLKLDPQEKNLYLYMLGVCYYQIGKYSDAVLYLAQISDSRILLDSDRYLILSYLALGEKDRVIA", "aa_hexdigest": "f0905ca8bcc9becb93bc4d4d32082be0", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13428.12", "name": "TPR_14", "length": 37, "aa_cov": 0.29133858267716534, "hmm_cov": 1.0, "evalue": 1.863824605496052e-06, "score": 24.657896041870117, "start": 41, "stop": 77 }, { "id": "PF14559.12", "name": "TPR_19", "length": 52, "aa_cov": 0.4094488188976378, "hmm_cov": 0.9807692307692307, "evalue": 3.0063842706835025e-08, "score": 30.374164581298828, "start": 50, "stop": 101 }, { "id": "PF07719.23", "name": "TPR_2", "length": 23, "aa_cov": 0.18110236220472442, "hmm_cov": 1.0, "evalue": 7.590855324208614e-10, "score": 34.761024475097656, "start": 75, "stop": 97 }, { "id": "PF13174.12", "name": "TPR_6", "length": 24, "aa_cov": 0.1889763779527559, "hmm_cov": 1.0, "evalue": 1.653748258882355e-08, "score": 31.18899917602539, "start": 76, "stop": 99 } ], "seq_stats": { "molecular_weight": 14540.743399999987, "isoelectric_point": 6.039108848571778 }, "id": "NKOCPLAKPK_284", "locus": "GCA003260355_00243" }, { "type": "cds", "sequence": "contig_1", "start": 121810, "stop": 122184, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTCCGAAAACCTTACCGTAGAGGGGAAACTCAAGAGCGTTCAAGTGAATTGAGAAGCTTGACAGAAACCTATCTCAAGGCTTGCAAACAAAAGCTTTCAGGAGAGCACTTGGAGATCTGTCACTATGGAGAACTGGGAAATAAAGCTCAGCGTCATGCGATTACTGATGAAGATGAGCCGTCAATTAACCGTTTCGCTAGGAGAACGCCTAAACCTGAATTTTTCCAACTCTTAGGAGAACTGGCATTAGAACAGAATGAAAGTAAGAAGGCGACGACTGCTTTTATGAAGGCATTAGGACTTGCCTCTGATACTCAAGAAAAACTGCATCTGAAAAAACTGATTTTACAAGCAAATGACCTAGAAGAATAG", "aa": "MLRKPYRRGETQERSSELRSLTETYLKACKQKLSGEHLEICHYGELGNKAQRHAITDEDEPSINRFARRTPKPEFFQLLGELALEQNESKKATTAFMKALGLASDTQEKLHLKKLILQANDLEE", "aa_hexdigest": "64495b205ffa99cbbf0789ca17a37db8", "start_type": "ATG", "rbs_motif": "TTCTT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14286.079499999974, "isoelectric_point": 7.790955924987793 }, "id": "NKOCPLAKPK_285", "locus": "GCA003260355_00244" }, { "type": "cds", "sequence": "contig_1", "start": 122280, "stop": 122549, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGAGGAATCGTTAAAAGTAGGCCTTTCGTCAAAAGGACACGTAGAGGCCTTTCTTCAGGTGGAACACTTCCCTTTGTTAGGGGGAGAAGAAGGGGCAAGGATCAGCCTTCCTTTTTTTCTTCTTTCTTTAAAAAATGGGGACTTATCGGCCTTATTATACTACTTACCTCCGTTTTGATTGTTTTTCTCCTTAGAGGAACCCGATATAATCCTACCTATCTCATAAAAACCATCGAATATAGCCAAGAAACTAAAGCAAAATATTGA", "aa": "MRGIVKSRPFVKRTRRGLSSGGTLPFVRGRRRGKDQPSFFSSFFKKWGLIGLIILLTSVLIVFLLRGTRYNPTYLIKTIEYSQETKAKY", "aa_hexdigest": "7df0f95e36f4c7cf1f84debf28e56b5c", "start_type": "ATG", "rbs_motif": "TTATT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10302.108399999988, "isoelectric_point": 11.627598381042482 }, "id": "NKOCPLAKPK_286", "locus": "GCA003260355_00245" }, { "type": "cds", "sequence": "contig_1", "start": 123009, "stop": 123149, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTTTCCTTTTTAAAGAAGAATTGGACCATCAGCTTCAGAAATTTGCTCGGCTCAAAGGCTACTACAGTGGTTATAATTCGCTTACTACTGTTGATTTGGGAAGTTTGACTCAAGATAAAGTGATTGTAGGAAGGTAA", "aa": "MIFLFKEELDHQLQKFARLKGYYSGYNSLTTVDLGSLTQDKVIVGR", "aa_hexdigest": "eb73a0324a5e51d3a12279133051c995", "start_type": "ATG", "rbs_motif": "TTTTT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5324.070700000002, "isoelectric_point": 8.08551197052002 }, "id": "NKOCPLAKPK_287", "locus": "GCA003260355_00246" }, { "type": "cds", "sequence": "contig_1", "start": 123300, "stop": 124430, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACTGAACCTAGCCCAAAAACAGTCCCAGGAGAAAGAGATGTAGCTCCACAAGTAACTCAAGAAATGCCAACTCCAGCTCCAGAAGTATCTGAACCTAGCCCAAAAACAGTTCCAGGCGAAAGAGATGTAGCTCCACAAGTAACTCAAGAAATACCAACTCCAGCTCCAGAATCGTCTGAACCTAGCCCAAAAACAGTCCCAGGAGAAAGAGATGTAGCTCGACAAGTAACTCAAGAAATACCAACTCCAGCTCCAGAATCGTCTGAACCTAGCCCAAAAACAGTTCCAGGCGAAAGAGATGCAACTCCACAAGTAACTCAAGAAATACCAACTCCAGCAGCGCCAGAAGCATCAACCAGAGCGCCAGAGATGCCAACTCAAGCTCCAGAAACAACTGAACCTAGACCAGAAACAGTTCCAGGAGAAAGAAATGTAGCTCCACAAGTAACTCAAGAAATACCAACTCCAGCAGCGCCAGAAGCATCAACCAGAGCGCCAGAGATGCCAACTCAAGCTCCAGAAACAACTGAACCTAGACCAGAAACAGTCCCAGGAGAAAGAGATGTAGCTCCACAGGTAACTCAAGAAATGCCAACTCCAGCTCCAGAAGCATCTGAACCTAGCCCAAAAACAGTTCCAGGCGAAAGAGATGTAGCTCCACAAGTAACTCAAGAAATACCAACTCCAGCTCCAGAATCGTCTGAACCTAGCCCAAAAACAGTCCCAGGCGAAAGAGATGCAGCTCCACAAGTAACTCAAGAAATACCAACTCCAGCAGCGCCAGAAGCATCAACCAGAGCGCCAGAGATGCCAACTCCAGCTCAAGAAGCGTCAACCAGAGCACCAGAGATGCCAACTCCAGCGCCAGAAACAACTGAACCTAGACCAGAAACAGTTCCAGGAGAAAGAGATGTAGCTCCACAAGTAACTCAAGAAATACCAACTCCAGCTCCAGAGGCATCAACCAGAGCGCCAGAGATGCCAACTCAAGCTCCAGAAACAACTGAACCTAGACCAGAAACAGTCCCAGGAGAAAGAGATGTAGCTCCACAGGTAACTGAAGAAATACCAACTCCAGCTCCAGAGGCATCAACTAGGGCTCCAGAAAGACCAGAACCAACAATATAA", "aa": "MTEPSPKTVPGERDVAPQVTQEMPTPAPEVSEPSPKTVPGERDVAPQVTQEIPTPAPESSEPSPKTVPGERDVARQVTQEIPTPAPESSEPSPKTVPGERDATPQVTQEIPTPAAPEASTRAPEMPTQAPETTEPRPETVPGERNVAPQVTQEIPTPAAPEASTRAPEMPTQAPETTEPRPETVPGERDVAPQVTQEMPTPAPEASEPSPKTVPGERDVAPQVTQEIPTPAPESSEPSPKTVPGERDAAPQVTQEIPTPAAPEASTRAPEMPTPAQEASTRAPEMPTPAPETTEPRPETVPGERDVAPQVTQEIPTPAPEASTRAPEMPTQAPETTEPRPETVPGERDVAPQVTEEIPTPAPEASTRAPERPEPTI", "aa_hexdigest": "16042b7eb3125bf7b2e7d05c1876a50a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 39723.149900000055, "isoelectric_point": 4.266300773620606 }, "id": "NKOCPLAKPK_288", "locus": "GCA003260355_00247" }, { "type": "cds", "sequence": "contig_1", "start": 124836, "stop": 125147, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00266.25" ], "nt": "ATGAATAAAAAAGATTTTCCTCTTTTGATAAAGTATCCGGAAATTACCTATCTTGATAATGCTGCTACTACCCAGAAACCTGCCCAAGTTATTCATGGTGTTTCACAGTTTGTGGAAAATGAATATGCGAATATTCATAGAGGGCTCTATGCACTTTCTGAAGCTTCAGAGCTTCACTATTATCAGAGTAAAAAGCTGGTTGCAGAGCTTATTCATTGTCTGCCCAAAGAGATTATTTATACTGCGAATGCGACTGCTTCGATCAATCTGTTGGCGCAAAGTCTAGCAAATTCTGGTTTTCTTGGAAAATGA", "aa": "MNKKDFPLLIKYPEITYLDNAATTQKPAQVIHGVSQFVENEYANIHRGLYALSEASELHYYQSKKLVAELIHCLPKEIIYTANATASINLLAQSLANSGFLGK", "aa_hexdigest": "661f7b5bc9830f31ad79613f5718117e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00266.25", "name": "Aminotran_5", "length": 84, "aa_cov": 0.8058252427184466, "hmm_cov": 1.0, "evalue": 1.11338286992833e-21, "score": 73.6413345336914, "start": 16, "stop": 98 } ], "seq_stats": { "molecular_weight": 11497.041299999983, "isoelectric_point": 6.896695518493654 }, "id": "NKOCPLAKPK_289", "locus": "GCA003260355_00248" }, { "type": "cds", "sequence": "contig_1", "start": 125154, "stop": 125369, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00266.25" ], "nt": "GTGCTGGTAAGTATTTGGGACCATCATGCGAATATTCTCCCATGGATGGCACTTTCCAAGCATTTTGGGTTTACTGTTAAATTCTTTGGGTTGACAGGGGATTTTGAAATTGATTTTGAAGATTTTCATCAGAAATATACCAAAGAGGTAAAAGTAGTGGCTTGTAGTCAGATTTCAAATGTGACGGGTGGAATTTATGATATGAAAAAGATTTGA", "aa": "MLVSIWDHHANILPWMALSKHFGFTVKFFGLTGDFEIDFEDFHQKYTKEVKVVACSQISNVTGGIYDMKKI", "aa_hexdigest": "bd506f9fe892e1a1982f1196d40185c6", "start_type": "GTG", "rbs_motif": "TTCTT", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00266.25", "name": "Aminotran_5", "length": 70, "aa_cov": 0.9859154929577465, "hmm_cov": 1.0, "evalue": 1.4331157068387312e-14, "score": 50.245819091796875, "start": 2, "stop": 71 } ], "seq_stats": { "molecular_weight": 8211.427099999999, "isoelectric_point": 6.380256462097168 }, "id": "NKOCPLAKPK_290", "locus": "GCA003260355_00249" }, { "type": "cds", "sequence": "contig_1", "start": 125817, "stop": 126119, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATACTCAGAACAAGTATATATTTTTCCCACGTAATAGAAAGGAAAAAACTTTTCCCTATTCCTTTAATTGTTTATAAATTCCTCCAAGGTCTCAAAAAACTTATCCAAATCAGAGGTATCATTATAGAGATAGGCACTCATTCTACAGGTACCTCCAAGTCAAAATGCCTTATGCAAAGGATAGGCACAATGCCCACCACAACGGATACAGATATTTCTTTCAGCAAAAAACTCTCAAACCTGATTAAAGTTTTCTTTCCCTTTAACGATAAAGGAAAAGAGAGCAACTCTTTCTTCTGA", "aa": "MILRTSIYFSHVIERKKLFPIPLIVYKFLQGLKKLIQIRGIIIEIGTHSTGTSKSKCLMQRIGTMPTTTDTDISFSKKLSNLIKVFFPFNDKGKESNSFF", "aa_hexdigest": "9d2e4acfebc85fbba45c7c9fb568693b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11473.539899999983, "isoelectric_point": 10.12716121673584 }, "id": "NKOCPLAKPK_291", "locus": "GCA003260355_00250" }, { "type": "cds", "sequence": "contig_1", "start": 126167, "stop": 127123, "strand": "+", "frame": 2, "gene": null, "product": "Polysaccharide biosynthesis protein C-terminal domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A288DNL8", "UniRef:UniRef90_A0A328EFC6" ], "nt": "GTGATAAAAGATGCTTTACGACAGCTTTTTGGAAGAATTGTAAGTGCTTTATTTGGTTTTGTGATTACCAAAATTATTGCTTCTTATCTTGGACCTCTGAGGTATGGAGATTATGGGACGATTTTCAGATTTTTTGCCTGGTGGACGGCTCTTGTGGATTTTGGAATCTATGTGATTGCAGTCAAACAGCTTGGGCAGATCAAAAACACGACTTATGGTGAGGGAGAAAATATTCCAAAACCTGACGCTGATTCTGAGTTGGCTATGACTTACGGAAAGTTTGTTGGAATGAGGGCATTTCTTATTTTTATTATCTATACCTTGGCAATCGTTGTTGCGTATTTGATCCCTGCATATACGAGTAATCCGTTTATTGTGCGAGGCTTGCCGCTTGCGATGTGTTATTCTGCGAGTAATATGTTTGCTGGGATTCAGCAGCTTCCTCTTCAAATCTTCCGAAAGATGAAAAGACTGACCTGGTCGCTGATTATTGCGAGGTTTTCTCAGCTTTTCGTCTTGGTTCCCGTAGTGTTTCTTTTCTTCAAAAATATGAATTTTGAAGAGAGCGGAAATCCTGATAGTATGGCGATTTTGGCTTTTACTTTAGTGATTTTTTCTGTGGTGGCGAGTTCTATTGGTCAAAATGTGGAAATTCATCTAAGGACGAAAGATTTACTTCCATTGAAGATCAGATTTGACTTTTCTTTTATCAAAAATCTGCTTTCCAAAAACCGAAAATACGGCTTTTCCTACTTTTTTAGTAGTTTTCATACCTTATTGGTGCTGATGTTTCTTGGGCGGTTTTATCCGACGGCAAGCGGATTTAAATATGCAGGAATTTGGGCTTTAGCACTTTCTTTGATTGAGATCCTTTTGATTGTTCCTTCTTCTCTGGGAAATAGCTTGCTTCACAAAATCCCGCATTATTCTGCTGAAAACAAAAAGAAATCTCTCTGA", "aa": "MIKDALRQLFGRIVSALFGFVITKIIASYLGPLRYGDYGTIFRFFAWWTALVDFGIYVIAVKQLGQIKNTTYGEGENIPKPDADSELAMTYGKFVGMRAFLIFIIYTLAIVVAYLIPAYTSNPFIVRGLPLAMCYSASNMFAGIQQLPLQIFRKMKRLTWSLIIARFSQLFVLVPVVFLFFKNMNFEESGNPDSMAILAFTLVIFSVVASSIGQNVEIHLRTKDLLPLKIRFDFSFIKNLLSKNRKYGFSYFFSSFHTLLVLMFLGRFYPTASGFKYAGIWALALSLIEILLIVPSSLGNSLLHKIPHYSAENKKKSL", "aa_hexdigest": "3c7c9aaaeadfa9631cf6e8e3cd93dca2", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 126149, "stop": 127426, "observations": { "mutation": [ 126155, 127121 ], "stop": [ 126155, 127121 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EFC6", "query_cov": 1.0, "subject_cov": 0.6080305927342257, "identity": 0.9840000000000001, "score": 605.0, "evalue": 6.09e-215, "gene_start": 1, "gene_end": 318, "reference_start": 7, "reference_end": 324, "reference_sequence": "MSGHKLVIKDALRQLFGRIVSALFGFVITKIIASYLGPLRYGDYGTIFRFFAWWTALVDFGIYVIAVKQLGQIKNTTYGEGENIPKPDADSELAMTYGKFVGMRAFLIFIIYSLAIVVAYLIPAYTSNPFIVRGLPLAMCYSASNMFAGIQQLPLQIFRKMKRLTWSLIIARFSQLFVLIPVVFLFFKNMNFEESGNPDSMTILAFALVIFSVVASSIGQNVEIHLRTKDLLPLKIRFDFSFIKNLLSKNRKYGFSYFFSSFHTLLVLMFLGRFYPTASGFKYAGIWALALSLIEILLIVPSSLGNSLLHKIPHYSAENKKKSLGNLMLLVVWIGVVIAMNFWIFADDIIVFVSSQKFLGTWENFTSWGSNQILPFLGIVLVLSFIKQVYNYLFVALEKQNLLFEINLVGVLIGAGVGVRLIPTYGLMGGVITQILIELIFTLGAIGLGIRKQLTPQLPKKVGIQLLGILVFFALIGVGIHYYFYARGVSMVRFFLVAGIFNLLLVAASLKAMKKVAKGLTND" }, "paralog": false, "identity": 0.9858823529411764, "score": 825.0, "evalue": 3.63e-304, "description": "Internal stop codon at 126155, 127121. Nonsense mutation around 126155, 127121.", "psc": { "uniref90_id": "UniRef90_A0A328EFC6", "product": "Polysaccharide biosynthesis protein C-terminal domain-containing protein", "uniref50_id": "UniRef50_A0A288DNL8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EFC6", "UniRef:UniRef50_A0A288DNL8" ] }, "pscc": { "uniref50_id": "UniRef50_A0A288DNL8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A288DNL8" ], "product": "Polysaccharide biosynthesis protein" } }, "id": "NKOCPLAKPK_292", "locus": "GCA003260355_00251" }, { "type": "cds", "sequence": "contig_1", "start": 127130, "stop": 127435, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTTTGGTGGTTTGGATTGGGGTTGTGATTGCGATGAACTTTTGGATTTTTGCAGATGATATTATTGTGTTTGTTTCTAGTCAAAAGTTTCTTGGAACCTGGGAAAACTTCACTTCCTGGGGATCTAATCAGATTTTGCCTTTTTTGGGTATTGTTTTGGTTTTGAGCTTTATCAAGCAGGTTTATAATTATCTTTTTGTTGCTCTTGAAAAGCAGAATCTGCTCTTTGAGATCAATTTGGTCGGAGTGCTGATTGGAGCAGGAGTTGGAGTGCGACTTATTCCTACTTATGGTTTGATGTGA", "aa": "MLLVVWIGVVIAMNFWIFADDIIVFVSSQKFLGTWENFTSWGSNQILPFLGIVLVLSFIKQVYNYLFVALEKQNLLFEINLVGVLIGAGVGVRLIPTYGLM", "aa_hexdigest": "ba6772c99e52b4bf443444f51a77eb8c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11386.511999999988, "isoelectric_point": 4.93575038909912 }, "id": "NKOCPLAKPK_293", "locus": "GCA003260355_00252" }, { "type": "cds", "sequence": "contig_1", "start": 127559, "stop": 127720, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTTTTTGCTTTGATTGGAGTAGGAATTCATTATTATTTCTATGTGAGGGGAGTAAGTATGGTTCGTTTTTTCTTGGTGGCTGGAATCTTCAACCTTCTCCTTGTTGCTGCGAGTTTAAAAGCTATGAAAAAGGTCGCGAAAGGATTGACGAATGATTAG", "aa": "MFFALIGVGIHYYFYVRGVSMVRFFLVAGIFNLLLVAASLKAMKKVAKGLTND", "aa_hexdigest": "f4827fabd69c7786a5272f9056a5772f", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5902.1336, "isoelectric_point": 10.118586921691893 }, "id": "NKOCPLAKPK_294", "locus": "GCA003260355_00253" }, { "type": "cds", "sequence": "contig_1", "start": 127754, "stop": 128134, "strand": "+", "frame": 2, "gene": null, "product": "Glycerophosphoryl diester phosphodiesterase membrane domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MVM6", "UniRef:UniRef90_A0A328EHI2" ], "nt": "ATGTTTAAAAAGATTTTACAAGCTTCAGCACAATCCTTGCTTTCTAGGCCAAAAATTATGAGGTTGGCTTTTTTTACCTTGGTTTGTTTTTCTATTGTTCGTTTGTATTATCTTATCTATTATTTTAATACTATTCTTATATGGAAATATGAGTCAGGAGTTCAGATTTCTGATGCTTTGATGTACTTTATCAATACGTTAAATGAGCACCATGCCATTGGTACCATTATCGTTGCAATCATTATTATTATTGTAGGTTATCTCTGGCTTTATCCGATTGGGCAGGCTTCTGTGATTTTTGCGCTTCAAGAACCTGAGAAATCTAATTTTTCTGCTTTTTCCAAAGGATTAGGGAGATTTTTTCCGATGCTAGAATATTGA", "aa": "MFKKILQASAQSLLSRPKIMRLAFFTLVCFSIVRLYYLIYYFNTILIWKYESGVQISDALMYFINTLNEHHAIGTIIVAIIIIIVGYLWLYPIGQASVIFALQEPEKSNFSAFSKGLGRFFPMLEY", "aa_hexdigest": "10548e4e518316a530da284c2236f1df", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 127754, "stop": 128437, "observations": { "mutation": [ 128132, 128423 ], "stop": [ 128132, 128423 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EHI2", "query_cov": 1.0, "subject_cov": 0.4329896907216495, "identity": 1.0, "score": 243.0, "evalue": 4.98e-79, "gene_start": 1, "gene_end": 126, "reference_start": 1, "reference_end": 126, "reference_sequence": "MFKKILQASAQSLLSRPKIMRLAFFTLVCFSIVRLYYLIYYFNTILIWKYESGVQISDALMYFINTLNEHHAIGTIIVAIIIIIVGYLWLYPIGQASVIFALQEPEKSNFSAFSKGLGRFFPMLEYGGLSVPFGLFTFSTVVLRLYLIDVLGNVVIDILIGIRGIMVLFASIFWSYAKVIIILEGVQVFDAIKKSTKLAFLNLGLSIKLMFVEFILLFRFLITGALIVGLPLGLVYLSVWLNILDNRFVEGLIRVIAGILLLILSYINCIVETFFLTYWYQAYQMISKKEE" }, "paralog": false, "identity": 0.986784140969163, "score": 431.0, "evalue": 2.89e-155, "description": "Internal stop codon at 128132, 128423. Nonsense mutation around 128132, 128423.", "psc": { "uniref90_id": "UniRef90_A0A328EHI2", "product": "Glycerophosphoryl diester phosphodiesterase membrane domain-containing protein", "uniref50_id": "UniRef50_A0A660MVM6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EHI2", "UniRef:UniRef50_A0A660MVM6" ] }, "pscc": { "uniref50_id": "UniRef50_A0A660MVM6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MVM6" ], "product": "Glycerophosphoryl diester phosphodiesterase membrane domain-containing protein" } }, "id": "NKOCPLAKPK_295", "locus": "GCA003260355_00254" }, { "type": "cds", "sequence": "contig_1", "start": 128144, "stop": 128425, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCCTTTTGGACTATTTACGTTTTCTACAGTAGTCTTGAGGCTCTATCTTATTGATGTGTTGGGGAATGTCGTGATAGATATTCTGATTGGAATCCGAGGAATTATGGTTTTGTTTGCCAGTATTTTCTGGTCTTATGCGAAGGTAATCATTATCTTGGAAGGTGTTCAGGTATTTGATGCGATAAAAAAAAGTACGAAGCTTGCTTTTCTTAATCTCGGACTTTCAATTAAATTAATGTTTGTGGAATTTCTCCTGCTTTTTAGGTTTTTGATTACTTGA", "aa": "MPFGLFTFSTVVLRLYLIDVLGNVVIDILIGIRGIMVLFASIFWSYAKVIIILEGVQVFDAIKKSTKLAFLNLGLSIKLMFVEFLLLFRFLIT", "aa_hexdigest": "ec8e79a6f4df596719df31682fc68068", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10561.889299999988, "isoelectric_point": 9.599229621887208 }, "id": "NKOCPLAKPK_296", "locus": "GCA003260355_00255" }, { "type": "cds", "sequence": "contig_1", "start": 128993, "stop": 129481, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAATTTCCATGACAAAGGATGAAGAAATAGCATTAGAACTCACAAAAGTCAACAACACCACTCAGGTCAAAAATGCAGGAGCACCACTTCTCATCAAAAATAAAAAGACAAAGACTGAAGAAGCTCCTACAAAACTTGAAACTGCAAAATTATTGACAGTTCAAGATAATGATATCGCAAAGATCACAGATATTGAAACACTCGAAGAAGTATTAAGTACCAAAAACAACCTAACACATACTGTGACACTTGCTCAAAATACCACAGGACAGCAACTTTTATCTGGTGGAATTCCTTCTCCTGAAGAACCATCTATCCTTTCTGGAGATACGAGCTTTTTGGCAAGTAATCATACCGAGGTAAGTTCTGGAAATCAGGAATCAATTACCTCAGATCTAGCTTATCAGTCAGATGAGAAGTCTATTCCAACAGAAACTCAATTGAGTCAAATCACAGCTGCACTCAATCCAAACTTTATTCTCTGA", "aa": "MAISMTKDEEIALELTKVNNTTQVKNAGAPLLIKNKKTKTEEAPTKLETAKLLTVQDNDIAKITDIETLEEVLSTKNNLTHTVTLAQNTTGQQLLSGGIPSPEEPSILSGDTSFLASNHTEVSSGNQESITSDLAYQSDEKSIPTETQLSQITAALNPNFIL", "aa_hexdigest": "9cf5c462f5e9fbad5a74360b0d7f9422", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17424.259899999994, "isoelectric_point": 4.578801536560059 }, "id": "NKOCPLAKPK_297", "locus": "GCA003260355_00256" }, { "type": "cds", "sequence": "contig_1", "start": 129788, "stop": 130045, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATTTACATAACTATTTTACACAGCAATCAAGACAATATCTTCCAGATAATAGGAAGCTTGCACTCTATGAAAAGATCTGCTACCAGAGATCAGGATTTTCTCCTTCACTCAAAAGATCTAAAATTCTGACAAAAAAAAGAGTATATGCCTTCCTTTCTTTTGTACTATTTTTTGCTTGTTTTGGTACTTTTTTCTGGGAAGACACAAGACTTGTTGATTACAGAGCCTTCTTTACACAAAAAGGGTTCTTTTGA", "aa": "MNLHNYFTQQSRQYLPDNRKLALYEKICYQRSGFSPSLKRSKILTKKRVYAFLSFVLFFACFGTFFWEDTRLVDYRAFFTQKGFF", "aa_hexdigest": "e7333796fb2e4ffb25382ff179d45c50", "start_type": "ATG", "rbs_motif": "TTCTA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10367.900699999991, "isoelectric_point": 9.95187168121338 }, "id": "NKOCPLAKPK_298", "locus": "GCA003260355_00257" }, { "type": "tRNA", "sequence": "contig_1", "start": 130300, "stop": 130372, "strand": "-", "gene": "trnK", "product": "tRNA-Lys(ctt)", "amino_acid": "Lys", "anti_codon": "ctt", "score": 73.1, "nt": "GGGAGTGTAGCTTAGTTGGTAGAGCAGATCCCTCTTAAGGATAAGGTCGTGGGTTCGAATCCCACCCCTCCCA", "db_xrefs": [ "SO:0000265" ], "anti_codon_pos": [ 130360, 130362 ], "id": "NKOCPLAKPK_29", "locus": "GCA003260355_00258" }, { "type": "cds", "sequence": "contig_1", "start": 130525, "stop": 130818, "strand": "-", "frame": 1, "gene": null, "product": "DUF4190 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EJ18", "UniRef:UniRef90_A0A328EJ18" ], "nt": "ATGCTAGATGGAACCTGTAAATTCAAAGCTTACGAAGCTACAGACATCAGAAAAGGAAACAGAGACACCTCTGCAATGGGCTTTCTCCAAGATGCGGTATTGGCTCCCGTCATTTTTATCGGAACACTAGTGACTGTCGCTCTAATTGTCTCAGGTCTTCTCTACGTTTTCTCCGGAGCAGAAAGTTCGCTCAAGAGCAAAGCAAAAAACGGAATTACCTATTCTATAGTCGGGCTTGTGGTAGTAATGCTATCCTTAGTTATTATCAGATTTGTGCAGTTTTTGGCGAGATGA", "aa": "MLDGTCKFKAYEATDIRKGNRDTSAMGFLQDAVLAPVIFIGTLVTVALIVSGLLYVFSGAESSLKSKAKNGITYSIVGLVVVMLSLVIIRFVQFLAR", "aa_hexdigest": "ca38fd4176cde5cf0ae2467928dd1373", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 130516, "stop": 130929, "observations": { "mutation": [ 130527, 130842 ], "stop": [ 130527, 130842 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EJ18", "query_cov": 1.0, "subject_cov": 0.708029197080292, "identity": 1.0, "score": 175.0, "evalue": 6.47e-55, "gene_start": 1, "gene_end": 97, "reference_start": 38, "reference_end": 134, "reference_sequence": "MLLKIKKIWFSFFCLAPLLMGITNASENFGIDLNTECMLDGTCKFKAYEATDIRKGNRDTSAMGFLQDAVLAPVIFIGTLVTVALIVSGLLYVFSGAESSLKSKAKNGITYSIVGLVVVMLSLVIIRFVQFLARGGS" }, "paralog": false, "identity": 0.9854014598540146, "score": 259.0, "evalue": 2.83e-90, "description": "Internal stop codon at 130527, 130842. Nonsense mutation around 130527, 130842.", "psc": { "uniref90_id": "UniRef90_A0A328EJ18", "product": "DUF4190 domain-containing protein", "uniref50_id": "UniRef50_A0A328EJ18", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EJ18", "UniRef:UniRef50_A0A328EJ18" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EJ18", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EJ18" ], "product": "DUF4190 domain-containing protein" } }, "id": "NKOCPLAKPK_299", "locus": "GCA003260355_00259" }, { "type": "cds", "sequence": "contig_1", "start": 130871, "stop": 131020, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTAAGGGAGCCAAACAAAAGAATGAAAACCAGATTTTTTTTATCTTTAGAAGCATGGTCTTTGGAAAAATAAATAATGTTATTCTTCTGATTTACCGAAGTGAGAAGATTATTGAGCTCCTGCGCTTCAATTTCCCAAAAGCATAA", "aa": "MSKGAKQKNENQIFFIFRSMVFGKINNVILLIYRSEKIIELLRFNFPKA", "aa_hexdigest": "f9e8fbadb724c3a8a3d066c57ca6b9ed", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5835.928699999998, "isoelectric_point": 10.377040672302243 }, "id": "NKOCPLAKPK_300", "locus": "GCA003260355_00260" }, { "type": "cds", "sequence": "contig_1", "start": 131020, "stop": 131259, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGCTACCAAAAATAAGCGAAACGGCAGCAATTATAAGGATAATAATCGTAACCCATTTCATAACAGGATTTTTTTTCATACAAAAAAGGTTAATACAAGATAAAACATCAACAGACTACAGTTTTAACTATAAAAATCAAGCTCTTTTTATTTTTCTTCTTACTTTCAGTTGCATTAGAAAAACATTTTCTTATATATTACTCGTTGATCTAGGGGATACGCCTAGTGATAGGTGA", "aa": "MKLPKISETAAIIRIIIVTHFITGFFFIQKRLIQDKTSTDYSFNYKNQALFIFLLTFSCIRKTFSYILLVDLGDTPSDR", "aa_hexdigest": "2a6ecbabeb6133ed9cccd82a9c0b1118", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9228.773399999991, "isoelectric_point": 9.516129875183104 }, "id": "NKOCPLAKPK_301", "locus": "GCA003260355_00261" }, { "type": "tRNA", "sequence": "contig_1", "start": 131556, "stop": 131627, "strand": "+", "gene": "trnW", "product": "tRNA-Trp(cca)", "amino_acid": "Trp", "anti_codon": "cca", "score": 67.5, "nt": "AGGGCCGTAGCTCAGTGGTAGAGCGGGAGTCTCCAAAGCTCCGCGTCGCGGGTTCAATTCCTGCCGGCCCTG", "db_xrefs": [ "SO:0000271" ], "anti_codon_pos": [ 131588, 131590 ], "id": "NKOCPLAKPK_9", "locus": "GCA003260355_00262" }, { "type": "tRNA", "sequence": "contig_1", "start": 131635, "stop": 131705, "strand": "+", "gene": "trnR", "product": "tRNA-Arg(tcg)", "amino_acid": "Arg", "anti_codon": "tcg", "score": 57.2, "nt": "GGGGGGTTGGTGTAGTGGTAACACATCGGTCTTCGGAACCGCTGTCGGGGGTTCGATCCCCTCACTCCCTG", "db_xrefs": [ "SO:0001036" ], "anti_codon_pos": [ 131660, 131662 ], "id": "NKOCPLAKPK_10", "locus": "GCA003260355_00263" }, { "type": "tRNA", "sequence": "contig_1", "start": 131818, "stop": 131898, "strand": "+", "gene": "trnL", "product": "tRNA-Leu(gag)", "amino_acid": "Leu", "anti_codon": "gag", "score": 49.9, "nt": "GCTGAGGTGATGGAATTGGTATACATGTACGCTTGAGGTGCGTATGCCTTTGGCTTGAGGGTTCGAGTCCCTTCCTCAGCA", "db_xrefs": [ "SO:0000264" ], "anti_codon_pos": [ 131821, 131823 ], "id": "NKOCPLAKPK_11", "locus": "GCA003260355_00264" }, { "type": "cds", "sequence": "contig_1", "start": 131911, "stop": 132147, "strand": "-", "frame": 1, "gene": null, "product": "ABC transmembrane type-1 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EJ89", "UniRef:UniRef90_A0A328EJ89" ], "nt": "GTGCTTATCGCATTTTTCAAGGTTTCTGAGCTGAATATCGTAGCATTTTTAGCAAAGTTCATCCAGAATCATTTTTTTGATACGACAGAGAAATACCAGACAAACTTTGCGAAGACCAGCCAAACTGATATTGCTATAGAAAAATTTAAAATTCAGAATACTAAACAAAAAATCGACTACAAAACAAAGTTGGAACTCAACAATTTTGATAAAATTGACACCGCAGGACTTTTATGA", "aa": "MLIAFFKVSELNIVAFLAKFIQNHFFDTTEKYQTNFAKTSQTDIAIEKFKIQNTKQKIDYKTKLELNNFDKIDTAGLL", "aa_hexdigest": "b6037b9e304a119e928facd00b89b07b", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 131905, "stop": 132348, "observations": { "mutation": [ 131913, 132201 ], "stop": [ 131913, 132201 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EJ89", "query_cov": 0.9871794871794872, "subject_cov": 0.5238095238095238, "identity": 1.0, "score": 147.0, "evalue": 3.72e-44, "gene_start": 2, "gene_end": 78, "reference_start": 69, "reference_end": 145, "reference_sequence": "MKAVFPKNIQKGLLSGLNISIGPLSISIIQLFILAIGIAIALVVFSKVSGSSKVIATFFAILIFLFFVLIAFFKVSELNIVAFLAKFIQNHFFDTTEKYQTNFAKTSQTDIAIEKFKIQNTKQKIDYKTKLELNNFDKIDTAGLLGK" }, "paralog": false, "identity": 0.9863945578231292, "score": 272.0, "evalue": 9.71e-96, "description": "Internal stop codon at 131913, 132201. Nonsense mutation around 131913, 132201.", "psc": { "uniref90_id": "UniRef90_A0A328EJ89", "product": "ABC transmembrane type-1 domain-containing protein", "uniref50_id": "UniRef50_A0A328EJ89", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EJ89", "UniRef:UniRef50_A0A328EJ89" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EJ89", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EJ89" ], "product": "ABC transmembrane type-1 domain-containing protein" } }, "id": "NKOCPLAKPK_302", "locus": "GCA003260355_00265" }, { "type": "cds", "sequence": "contig_1", "start": 132199, "stop": 132348, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGCAGTATTCCCCAAAAATATTCAAAAAGGGCTTCTTTCTGGGCTTAATATTTCGATTGGTCCATTGAGCATTTCAATAATTCAGCTCTTTATACTAGCGATAGGTATTGCAATTGCGCTCGTCGTTTTTTCCAAAGTATCTTGA", "aa": "MKAVFPKNIQKGLLSGLNISIGPLSISIIQLFILAIGIAIALVVFSKVS", "aa_hexdigest": "ae44f4258c8e45e63d03c2bfd1b57fb6", "start_type": "ATG", "rbs_motif": "TTATT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5137.2997, "isoelectric_point": 10.477289009094235 }, "id": "NKOCPLAKPK_303", "locus": "GCA003260355_00266" }, { "type": "cds", "sequence": "contig_1", "start": 132417, "stop": 132842, "strand": "-", "frame": 2, "gene": "ruvC", "product": "Crossover junction endodeoxyribonuclease RuvC", "db_xrefs": [ "EC:3.1.21.10", "KEGG:K01159", "SO:0001217", "UniRef:UniRef50_A0A288DFS6", "UniRef:UniRef90_A0A328EFX7" ], "nt": "TTGATTACTGATGATTTAAAAATCATTGATTCCGGGATCCTACTCCAAAAACAAGTCGCTCCTCAGAGAGAAAACCAATTTGAAAGGATGATTCAGATTGCTAATTTTTTTGAAGAGCTTTTTGCAAAGTTTCAAATTCAGACTGTCGCAATGGAAAAGCTTTTCTTCACAAAATTTAATCAAAATAACGCAGAATTTGTATATGGAATTAGAGCAATTCTCATCACTATGGCAATGAAAAAGGGCTGCCATATAAAAGAATTCACTCCTATTGAACTAAAAAAATACATTACCGGCAACGGAAAAGCAGAAAAACTTCTCGTCCAGAAAATGACCATGAAGCTCTATAAACTCCAAGACCTTCCCGAATACAATGATGCAGCAGATGCTCTAGGACTCGCCTATCTTGCTATGAGAGTAAAATAA", "aa": "MITDDLKIIDSGILLQKQVAPQRENQFERMIQIANFFEELFAKFQIQTVAMEKLFFTKFNQNNAEFVYGIRAILITMAMKKGCHIKEFTPIELKKYITGNGKAEKLLVQKMTMKLYKLQDLPEYNDAADALGLAYLAMRVK", "aa_hexdigest": "acc59ba2262f65c4e85eee031b40402a", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A328EFX7", "query_cov": 1.0, "subject_cov": 0.9038461538461539, "identity": 0.986, "score": 272.0, "evalue": 4.05e-92, "valid": true, "ec_ids": [ "3.1.21.10" ], "uniref50_id": "UniRef50_A0A288DFS6", "kegg_orthology_id": "K01159" }, "pscc": { "uniref50_id": "UniRef50_A0A288DFS6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A288DFS6" ], "product": "Crossover junction endodeoxyribonuclease RuvC" }, "genes": [ "ruvC" ], "id": "NKOCPLAKPK_304", "locus": "GCA003260355_00267" }, { "type": "cds", "sequence": "contig_1", "start": 132959, "stop": 133360, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTATACCGTATTAAATAGAGATTTATTACTTTTTGTCTTGAGCGAATATCTTCCTATTTTGAGTGAAATCATCAATTCTTATCTCAGCACAGTAGTCTCCTATATCCTCAACATCCGCTTAAAAGAGGAAAGCGAAAAACTCGAACTAGAAACCAAAATCCTTGACGAAAAAGGAGAAAGAGAGGTAAAAAGCCTGAGTGGAGGACAAAGAACAATTTTGAAACTCGTTCGGATGCTCGCAATCTCCTCTTATCTCAAAACTGAAATGCTCTTTCTCGATGAAACCGTCAATAATCTTGATGTAGAAACCGTCGGAAAGGTTGCTCAACTTTTAGATGGATTTGTTAAACAAAGACAAATGAAATTTTATGTCATTACGCACAATACAGAGATTCAATGA", "aa": "MYTVLNRDLLLFVLSEYLPILSEIINSYLSTVVSYILNIRLKEESEKLELETKILDEKGEREVKSLSGGQRTILKLVRMLAISSYLKTEMLFLDETVNNLDVETVGKVAQLLDGFVKQRQMKFYVITHNTEIQ", "aa_hexdigest": "a3819058bbe3bd9268aed93ac25e7192", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15403.813199999993, "isoelectric_point": 5.261835670471191 }, "id": "NKOCPLAKPK_305", "locus": "GCA003260355_00268" }, { "type": "cds", "sequence": "contig_1", "start": 133613, "stop": 133990, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATCAATCAACAGCATTTTCAGCAAAGAGAAAAAGAAAAAAACCAGATTATTCAGGAGCAGGAAGCCTTATCTCAGAAAATTGCCATGCTAAAGCTGAGCGAAAAGAAACAAGAACTTGAGCAAGCCATCAAAGCATCTGAGAATACAACTGCCGCTCAAGAAATCCAAAAAAACCTGACCAACCAGATGGAAATAAACCAAAACAAAAGGGAGCTATTGAGAAAGTTTTTGCAAAGTTTCGACTACAAAGCATTTGAAACTACCGTAGGACAGCGAGAAACCCTCAATACTTCACTCAAAGAAATTGAAAGAAAGCTCCAAGAAAAAGAAAACTCCCTCGCACATAGAGATCAGTATCAACAGGAAAAAATCTGA", "aa": "MINQQHFQQREKEKNQIIQEQEALSQKIAMLKLSEKKQELEQAIKASENTTAAQEIQKNLTNQMEINQNKRELLRKFLQSFDYKAFETTVGQRETLNTSLKEIERKLQEKENSLAHRDQYQQEKI", "aa_hexdigest": "82c5e3571d4f18fb8dda45fde4049d60", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14935.662599999981, "isoelectric_point": 7.9129301071167 }, "id": "NKOCPLAKPK_306", "locus": "GCA003260355_00269" }, { "type": "cds", "sequence": "contig_1", "start": 134021, "stop": 134233, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGGAATACATTTCAAGCAGGAACATGAGCTCCTGCTCCAAAAAAAAGAAGCAGAAATACAAAATCAGCAAAGAGTCCGTTCACAGCTGCAAGAACAGCTAAAAGTTTTGACGGAACAGGAGCTTTTTTACCAAGGACAAGTAAAATCCCTTGCTGAGAGATTGGAACAATTTGATCTACAAACAAATCAAGATGCCAAATTTGATTGCTGA", "aa": "MGIHFKQEHELLLQKKEAEIQNQQRVRSQLQEQLKVLTEQELFYQGQVKSLAERLEQFDLQTNQDAKFDC", "aa_hexdigest": "a3628791f03e8fa9462c61c5dd5b1b6a", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8404.377199999995, "isoelectric_point": 5.277977943420409 }, "id": "NKOCPLAKPK_307", "locus": "GCA003260355_00270" }, { "type": "cds", "sequence": "contig_1", "start": 134264, "stop": 134752, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTACAACACTACAAAACATTGCAAACCCTACCGACCGCACAACCTTTTTTGGAAGAGGAAAGGAAAATTTTTGAAGAACTTGAAATAATGGGCGAGAAGCTCAGCATTCAGAACTTTTCTCTCGATTCCCAGTTAAAAAGTTTCGTTCCCCGTCTTCAGAAAGCAGTCGCTGAAAAGCAAGCTGAGTTATCTCAAAAGCAAGCAATATTTTCCGTGCGTTCAGAGCAAGAAAATAAGCTAAAAAAACAGATTCTCACCCATCAGGCAACCATTACCCAGAATAACGATCAGATTTCAAAGTTAGACAAGCTCCTTTCTGAGATTAAGATAGAAGAAATCACCAGCTACAAACAGCAAAGAGAAAGCATCCTCCTCCAGCAACAAAAACGAGAAGCGATCCCTGAGTTGAGCTTGATTTCCTCCTTTTACCACGAACAAAAAGCATATCTGAGTCTCCAGCCAAGAAGCGATTTCTCCCTTTGCTGA", "aa": "MLQHYKTLQTLPTAQPFLEEERKIFEELEIMGEKLSIQNFSLDSQLKSFVPRLQKAVAEKQAELSQKQAIFSVRSEQENKLKKQILTHQATITQNNDQISKLDKLLSEIKIEEITSYKQQRESILLQQQKREAIPELSLISSFYHEQKAYLSLQPRSDFSLC", "aa_hexdigest": "06ec272f76844739c31230cf5a8ab68c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18954.474599999998, "isoelectric_point": 6.8494054794311525 }, "id": "NKOCPLAKPK_308", "locus": "GCA003260355_00271" }, { "type": "cds", "sequence": "contig_1", "start": 134876, "stop": 135214, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTATCTCATTATCAGACGCCTCAAGCAAGGGAAATCAAAAGATTCGACCAGTTCGCAACTTTTTTCTTGCGAATGTACAGAAATACAGCTTCTAGAAATGCTCCAAACCAGAGCACTTATCGAAAAAAACCTTGATATTGAAGACGAACTTAGAGCTCATCACATTATTCCCGAGGAGATCGTCTTTAAAAATGAAACCGATTTACAGCAGCAGCTCACGAGTTTACTTCCACCCCAAGAGGTCTTTGAAAGCACAATTTTTCTCCTCCAAGATGCAGAGAATATCTTTGAAATGCAGCCAAGTCAGAGATTAGAGGTGCTAAAACACGTATTTTGA", "aa": "MYLIIRRLKQGKSKDSTSSQLFSCECTEIQLLEMLQTRALIEKNLDIEDELRAHHIIPEEIVFKNETDLQQQLTSLLPPQEVFESTIFLLQDAENIFEMQPSQRLEVLKHVF", "aa_hexdigest": "32185ed6691a5b679427f65243962602", "start_type": "GTG", "rbs_motif": "TTTA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13174.014099999986, "isoelectric_point": 4.793084526062012 }, "id": "NKOCPLAKPK_309", "locus": "GCA003260355_00272" }, { "type": "cds", "sequence": "contig_1", "start": 135441, "stop": 135563, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTTTCTTACTTCTCTTCGAAAATGGATCTGCCTCAAAGCTTCTTGAACTTGTAAATCTAGAGAAATTAGCTATGCTTGCTTCTCGAAAGCTTTTTATCTTGAAGAGAAAAGATGCTTGA", "aa": "MFFLLLFENGSASKLLELVNLEKLAMLASRKLFILKRKDA", "aa_hexdigest": "b4ed71f3bebf37258eb73269f0dad6cd", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4612.5869999999995, "isoelectric_point": 9.819711494445798 }, "id": "NKOCPLAKPK_310", "locus": "GCA003260355_00273" }, { "type": "cds", "sequence": "contig_1", "start": 135751, "stop": 135858, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAGGCTCAGGAAATTGAACAAATCAAAAATATCCTTGCGAATATTGAAGCTAGTCAGAAAAAAATCCCTTATCTTTCGGATCTTGAACAGCATCCGGTTTTTTGA", "aa": "MQAQEIEQIKNILANIEASQKKIPYLSDLEQHPVF", "aa_hexdigest": "2c2fabd10a0a1d9172d199239372e12e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4067.619600000001, "isoelectric_point": 4.957747077941893 }, "id": "NKOCPLAKPK_311", "locus": "GCA003260355_00274" }, { "type": "cds", "sequence": "contig_1", "start": 135898, "stop": 136209, "strand": "+", "frame": 1, "gene": null, "product": "CRISPR type III A-associated protein Csm2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7W4HGR6", "UniRef:UniRef90_A0A660MXL6" ], "nt": "GTGGAGGAGGTTATTAGGAGCTATATTTTAGGAAAGGTGGAAAGTATTCAAAAAACTAAAGGTGGGCAGCTTTTTGCCAGGTTTGTGGAATCGCAGTCTGAACTTTTTTGGAAGTTTCGTGAGGCAAATGATCCAAGCTATCAGGGCAAGGCATTTCAGAGCTTAGGTAAAGAAGTCGAGATGGAAATGTTTAAACTTGAGGGGATCCTTACTGAGAAAATGCTCAAACAGGAAAAGGGGCTTGATAAAGTGGTAGATTCTTTTTACAATATCATCTATCTTTTCTTTCCAAGATATAATGAGATTGAATAA", "aa": "MEEVIRSYILGKVESIQKTKGGQLFARFVESQSELFWKFREANDPSYQGKAFQSLGKEVEMEMFKLEGILTEKMLKQEKGLDKVVDSFYNIIYLFFPRYNEIE", "aa_hexdigest": "3849c4bd3668d1fdd0a72c5706eafc86", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 135751, "stop": 136209, "observations": { "mutation": [ 135856 ], "stop": [ 135856 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A660MXL6", "query_cov": 1.0, "subject_cov": 0.6776315789473685, "identity": 0.99, "score": 202.0, "evalue": 2.9e-65, "gene_start": 1, "gene_end": 103, "reference_start": 50, "reference_end": 152, "reference_sequence": "MQAQEIEQIKNILANIEASQKKIPYLSDLEQHPVFGPIFSQLTAGEKQEVEEVIRSYILGKVESIQKTKGGQLFARFVESQSELFWKFREANDPSYQGKAFQSLGKEVEMEMFKLEGILTEKMLKQEKGLDKVVDSFYNIIYLFFPRYNEIE" }, "paralog": false, "identity": 0.993421052631579, "score": 296.0, "evalue": 7.59e-105, "description": "Internal stop codon at 135856. Nonsense mutation around 135856.", "psc": { "uniref90_id": "UniRef90_A0A660MXL6", "uniref50_id": "UniRef50_A0A7W4HGR6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A660MXL6", "UniRef:UniRef50_A0A7W4HGR6" ] }, "pscc": { "uniref50_id": "UniRef50_A0A7W4HGR6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7W4HGR6" ], "product": "CRISPR type III A-associated protein Csm2" } }, "id": "NKOCPLAKPK_312", "locus": "GCA003260355_00275" }, { "type": "cds", "sequence": "contig_1", "start": 136434, "stop": 137324, "strand": "+", "frame": 3, "gene": null, "product": "Helicase ATP-binding domain-containing protein", "db_xrefs": [ "KEGG:K03702", "SO:0001217", "UniRef:UniRef50_A0A660MVC7", "UniRef:UniRef90_A0A660MVC7" ], "nt": "ATGTTTCAACTCAAATCTGCTTATCAGCCTGCCGGTGACCAACCTAAAGCAATCGCTCAGATTTCTGAAGCTTTTGATCAAGGGAAAAAGAAAATAACCTTGCTTGGTGCGACAGGAACTGGGAAGACTTTTACGATGGCTAATATTATTCAGAAGGTCCAGCAACCCACTTTGGTGATTTCTCACAATAAAACCCTTGCTGCCCAACTTGCAACAGAATTTAAGGCATTCTTTCCTGATAATGCGGTGCATTATTTCGTGTCTTATTTTGATTACTATCAGCCGGAGAGTTATTTGCCAAGTTCTGGAACCTATATTGAAAAAGAGGCAACGATCAATGACGAAATCGAGATGTATAGGCTCGCAACGATGGCTTCACTTCTGAGTAGAAAGGATGTAATTGTCGTGGCTTCTGCTTCTTCGCTTTACGGGCTCGGACAAAGCAGATTTTTTGAGGAGAATTGTCTTCAGCTCTATGTTGGCAAGAAATACGACTTCAATGAGCTGAAAAGACAGCTCCTTCATATGCAATATAAACCGATTCACGGGAAAATCGAGAAAGGAATGTTTGAGATGAGAGGAGATATTTTGGAGATTTTCTCTTCTACAGAAAGATGCCTGTATCGCTGTTTTTTTGATGAGGAATTTTTGGAAAGGATCGAGATGAGAGACGCCCAGTCCTACGAACTCAAAGCTCCAGTGCAGAAAGCGATGATCCGACCTGCGACGCAATATCTCCAAGATGTTTCAGACCTTGAAACGATTCTCCAGCAGATGGATGCTGAGAAAGAACTCCGTATCAAAGAATTTGAGAAAATGGGGATGAGTATTGAGGCAGAAAGAGTCAAAAAGAAAGTGGAATACGATATTAGAATGATTAGAGAAACCTGA", "aa": "MFQLKSAYQPAGDQPKAIAQISEAFDQGKKKITLLGATGTGKTFTMANIIQKVQQPTLVISHNKTLAAQLATEFKAFFPDNAVHYFVSYFDYYQPESYLPSSGTYIEKEATINDEIEMYRLATMASLLSRKDVIVVASASSLYGLGQSRFFEENCLQLYVGKKYDFNELKRQLLHMQYKPIHGKIEKGMFEMRGDILEIFSSTERCLYRCFFDEEFLERIEMRDAQSYELKAPVQKAMIRPATQYLQDVSDLETILQQMDAEKELRIKEFEKMGMSIEAERVKKKVEYDIRMIRET", "aa_hexdigest": "b8aa2d7d56a03fc36c74365deb9c43ee", "start_type": "ATG", "rbs_motif": "TTTTT", "psc": { "uniref90_id": "UniRef90_A0A0G1YVT0", "query_cov": 0.8209459459459459, "subject_cov": 0.9681274900398407, "identity": 0.502, "score": 228.0, "evalue": 4.83e-71, "valid": false, "product": "UvrABC system protein B", "uniref50_id": "UniRef50_A0A850PXB8", "kegg_orthology_id": "K03702" }, "genes": [], "truncated": "both", "pseudogene": { "start": 136315, "stop": 137627, "observations": { "deletion": [ 136393 ], "stop": [ 137323 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A660MVC7", "query_cov": 1.0, "subject_cov": 0.5183887915936952, "identity": 0.997, "score": 570.0, "evalue": 9.05e-201, "gene_start": 1, "gene_end": 296, "reference_start": 41, "reference_end": 336, "reference_sequence": "MQSAFFSFSFLAIPREISIVSGEILFFLCLICFYFLCFLVMFQLKSAYQPAGDQPKAIAQISEAFDQGKKKITLLGATGTGKTFTMANIIQKVQQPTLVISHNKTLAAQLATEFKAFFPDNAVHYFVSYFDYYQPESYLPSSGTYIEKEATINDEIEMYRLATMASLLSRKDVIVVASASSLYGLGQSRFFEENCLQLYVGKKYDFNELKRQLLHMQYKPIHGKIEKGMFEMRGDILEIFSSTERCLYRCFFDEEFLERIEMRDAQSYELKAPVQKAMIRPATQYLQDVSDLETILQQMDAEKELRIKEFEKMGMNIEAERVKKKVEYDIRMIRETGFVNGIENYSLYFDQRLPGQPPNTIFDYFPEDFLMIIDESHMTIPQLRAMAQGDRARKNNLIRYGFRLPSAIDHRPLRFEELEAVLGWKPLDQVALDEAAADLELVSSAHQQVLATQTEKEQKSDSSLLNREARKSNQHAVLAQKVKKDAKSIFLSATPAEYELELSEQVVEQIIRPTGLLDPITFVYPKSGDYQLLLDSLPKLLQKKPQLSTFLEEEPEQSATLPNFEELFGDE" }, "paralog": false, "identity": 0.988558352402746, "score": 850.0, "evalue": 1.16e-313, "description": "Internal stop codon at 137323. Frameshift due to deletion around 136393.", "psc": { "uniref90_id": "UniRef90_A0A660MVC7", "product": "Helicase ATP-binding domain-containing protein", "uniref50_id": "UniRef50_A0A660MVC7", "kegg_orthology_id": "K03702", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A660MVC7", "UniRef:UniRef50_A0A660MVC7", "KEGG:K03702" ] }, "pscc": { "uniref50_id": "UniRef50_A0A660MVC7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MVC7" ], "product": "Helicase ATP-binding domain-containing protein" } }, "id": "NKOCPLAKPK_313", "locus": "GCA003260355_00276" }, { "type": "cds", "sequence": "contig_1", "start": 137328, "stop": 138029, "strand": "+", "frame": 3, "gene": null, "product": "Helicase ATP-binding domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A351Y4M0" ], "nt": "GTGAATGGGATCGAGAATTATTCCCTCTACTTTGATCAGAGATTGCCCGGGCAACCACCGAATACGATCTTTGATTATTTTCCTGAGGATTTTTTGATGATTATTGATGAATCGCATATGACGATCCCTCAGCTCAGGGCAATGGCACAAGGGGATAGAGCAAGAAAAAATAATTTGATTCGTTATGGGTTCCGTTTACCCTCTGCAATTGACCATAGACCGTTAAGATTTGAGGAATTAGAAGCTGTCCTCGGCTGGAAACCCTTGGATCAAGTAGCTTTAGATGAGGCTGCCGCTGATTTGGAACTCGTTTCTAGTGCTCATCAGCAAGTCCTTGCAACCCAGACTGAAAAGGAGCAAAAATCAGATTCCAGTCTTTTGAATCGTGAGGCTAGAAAATCCAATCAACACGCCGTTTTGGCGCAGAAGGTAAAAAAGGATGCTAAATCTATCTTCCTTTCTGCGACGCCGGCTGAATATGAGCTGGAGCTTTCCGAGCAGGTCGTGGAACAGATTATTAGACCGACGGGCTTGCTGGATCCGATTACTTTTGTGTATCCTAAGTCTGGCGACTATCAACTTTTGCTGGATTCTCTCCCAAAATTGCTCCAAAAGAAACCGCAACTTTCTACTTTTCTTGAAGAAGAGCCTGAGCAGTCTGCAACGCTTCCAAATTTTGAGGAACTCTTCGGTGATGAGTAA", "aa": "MNGIENYSLYFDQRLPGQPPNTIFDYFPEDFLMIIDESHMTIPQLRAMAQGDRARKNNLIRYGFRLPSAIDHRPLRFEELEAVLGWKPLDQVALDEAAADLELVSSAHQQVLATQTEKEQKSDSSLLNREARKSNQHAVLAQKVKKDAKSIFLSATPAEYELELSEQVVEQIIRPTGLLDPITFVYPKSGDYQLLLDSLPKLLQKKPQLSTFLEEEPEQSATLPNFEELFGDE", "aa_hexdigest": "a9a3db36b5cfabeb996d388be1e778a9", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A351Y4M0", "query_cov": 0.8583690987124464, "subject_cov": 0.9615384615384616, "identity": 0.615, "score": 238.0, "evalue": 2.97e-76, "valid": false, "product": "Helicase ATP-binding domain-containing protein", "uniref50_id": "UniRef50_A0A351Y4M0" }, "pscc": { "uniref50_id": "UniRef50_A0A351Y4M0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A351Y4M0" ], "product": "Helicase ATP-binding domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_314", "locus": "GCA003260355_00277" }, { "type": "cds", "sequence": "contig_1", "start": 138296, "stop": 138811, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATAGGCTATCTTCAGCAAGAGGAAAGAATAAATGAGGAGCAAGAGGAAAGAATAAATGAGGAGCAAGAGGAAAGAGTAAATGAAGAGCAAGAGGAAAGAGTAGATGAGGAGCAAGAGGAAAGAGTAGATGAGGAGCAAGAGGAAAGAATAAATGAGGAGCAAGAGGAAAGAATAAATGAGGAGCAAGAGGAAAGAGTAAATGAAGAGCAAGAGGAAAGAGTAGATGAGGAGCAAGAGGAAAGAGTAGATGAGGAGCAAGAGGAAAGAATAAATGAGGAGCAAGAGGAAAGAATAAATGAGGAGCAAGAGGAAAGAGTAAATGAAGAGCAAGAGGAAAGAGTAGATGAGGAGCAAGAGGAAAGAGTAGATGAGGAGCAAGAGGAAAGAGTAGATGAGGAGCAAGAGGAAATAGAGAATGAGAAGCAAGAGGAAAGAGTAAATGAGAAGCAAGAGGAAAGAGTAAATGAGAAGAAAAAGAGTTTTTGGAAAAGACTTTTTGAACAATTTTATTAA", "aa": "MIGYLQQEERINEEQEERINEEQEERVNEEQEERVDEEQEERVDEEQEERINEEQEERINEEQEERVNEEQEERVDEEQEERVDEEQEERINEEQEERINEEQEERVNEEQEERVDEEQEERVDEEQEERVDEEQEEIENEKQEERVNEKQEERVNEKKKSFWKRLFEQFY", "aa_hexdigest": "0da318a8fc06fafa0205e255c7c190d1", "start_type": "TTG", "rbs_motif": "TTATT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 21854.233900000025, "isoelectric_point": 4.059634208679199 }, "id": "NKOCPLAKPK_315", "locus": "GCA003260355_00278" }, { "type": "cds", "sequence": "contig_1", "start": 138864, "stop": 139265, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTCGCTCATTCTCAGATGATCGCTCCTTTTCAGATGCTCACATCATTTCAGATTCCCGCTTTAGCTCAGATGGTTGCACCTTCTCAGATACTCGTTCGTTCTCAGATATTCGTTCCTTCTCAGATTTTCGCATTATTTCAGATGCTCGCTCTTTTTCAGATTTCCGCTCATTCTCAAATGCTCGCTCATCTTCAGATTTTCACATTATTTCAGATTTCCGTATCATTTCAGATTGTCGCATTCTTTCAGATTTCCACTCATTCTCTGATGTTCACATTCTTTCAGATGTTTGCTCCGTCACAGATTTGCGCATTCTTTCAGATGTTTGCTCCGTCCCAGATTTTCGCTCATTCTAAGATGTTCACTCATTTTCTGATGTTCGTATTAGCTCAAATATGA", "aa": "MFAHSQMIAPFQMLTSFQIPALAQMVAPSQILVRSQIFVPSQIFALFQMLALFQISAHSQMLAHLQIFTLFQISVSFQIVAFFQISTHSLMFTFFQMFAPSQICAFFQMFAPSQIFAHSKMFTHFLMFVLAQI", "aa_hexdigest": "899b651d1e8b5ba0603ae266291618c3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15288.14759999999, "isoelectric_point": 9.502140235900882 }, "id": "NKOCPLAKPK_316", "locus": "GCA003260355_00279" }, { "type": "cds", "sequence": "contig_1", "start": 139734, "stop": 140087, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTGTCAGAGAGTATTCTCACTCGATCTCAGATTATCACTCCAGCTCAGATTTCGATATTACGCCAGATTTGTGTATTAGCTCAGATTTTCGTATTATTTCATATTTCCGCATTATTTCATATTTCCACTAGATCTCAGATTATCACTCCAGCTCAGATTTTGTTGTCAGGAAACATTTTCACTAGATCTCAGATTATCACTCCAGCTCAGATTTTGTTATCAGGGGAAATTTTCACTCGAGATCAGATTATCACTCCAGCTCAGATTTCCGTATTAGCTCAGATTTTCGCATCACTTCAGATTTTCGCATTATTTCAGATTTCCACTCATTCTCAGATTTGTATCCTATAG", "aa": "MVSESILTRSQIITPAQISILRQICVLAQIFVLFHISALFHISTRSQIITPAQILLSGNIFTRSQIITPAQILLSGEIFTRDQIITPAQISVLAQIFASLQIFALFQISTHSQICIL", "aa_hexdigest": "44ef3f8d870640dc737436efc82de230", "start_type": "TTG", "rbs_motif": "TTTA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12974.255299999979, "isoelectric_point": 9.006571769714355 }, "id": "NKOCPLAKPK_317", "locus": "GCA003260355_00280" }, { "type": "cds", "sequence": "contig_1", "start": 140285, "stop": 140398, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00132.30" ], "nt": "GTGGATCCTTCAGCACAGATTGATCCTTCTGTTGTTATTCCAGAGGGCTGTGTTATTGGACCAAATGTCGTTATCGCAAAGGGGGCTGTTTTAGAAAGAAATGTTCATATTTGA", "aa": "MDPSAQIDPSVVIPEGCVIGPNVVIAKGAVLERNVHI", "aa_hexdigest": "4fcc60e6bd9cfe9aa8ae585b560b8fc5", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00132.30", "name": "Hexapep", "length": 28, "aa_cov": 0.7567567567567568, "hmm_cov": 1.0, "evalue": 2.48488123131623e-09, "score": 32.99836730957031, "start": 5, "stop": 32 } ], "seq_stats": { "molecular_weight": 3838.4543000000003, "isoelectric_point": 4.74920482635498 }, "id": "NKOCPLAKPK_318", "locus": "GCA003260355_00281" }, { "type": "cds", "sequence": "contig_1", "start": 140700, "stop": 141119, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EHJ0", "UniRef:UniRef90_A0A328EHJ0" ], "nt": "ATGGTCATTTCCTCTCAAAAACCAACGGTTCTGGCACAAAAAAGTCTATTTCCTCTCAAAAACCGACGGTTCTGGCACAAAAAAGTCTATTTCCTCTCAAAAACCGACGGTTCTTGGGCAAAAAAGTTCATTTCCTCTCAAAAACCAACGGTTCTTGGGCAAAAAAGTCTATTTCCTCTCAAAAACCGATGGTTCTTGGATAAAAAAGTTCATTTTCTCTCAAAAACCGATGATTCTGGAGGAAAAAGAGGGAAAAGAGATGAGGGGTTAGTGCGTTTTGAGAAGGAGGGGAATACTTTCTCCAAAAAATATAGAATACCACAGTATCCTGAAAAATTTAAGTCTAAAAAAAGATGGAAGTCAGATTTCTTTCTTTTTTCTTACTATAAAAAATGGAATTTAGAGATAGGAGAGCTGTGA", "aa": "MVISSQKPTVLAQKSLFPLKNRRFWHKKVYFLSKTDGSWAKKFISSQKPTVLGQKSLFPLKNRWFLDKKVHFLSKTDDSGGKRGKRDEGLVRFEKEGNTFSKKYRIPQYPEKFKSKKRWKSDFFLFSYYKKWNLEIGEL", "aa_hexdigest": "3c519bbc8067aa01d962d791ff42e328", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A328EHJ0", "query_cov": 1.0, "subject_cov": 0.9328859060402684, "identity": 1.0, "score": 198.0, "evalue": 5.37e-63, "valid": true, "uniref50_id": "UniRef50_A0A328EHJ0" }, "pscc": { "uniref50_id": "UniRef50_A0A328EHJ0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EHJ0" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16652.21929999999, "isoelectric_point": 10.31199207305908 }, "id": "NKOCPLAKPK_319", "locus": "GCA003260355_00282" }, { "type": "cds", "sequence": "contig_1", "start": 141142, "stop": 141897, "strand": "-", "frame": 1, "gene": null, "product": "CBS domain-containing protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBF4C30", "UniRef:UniRef100_A0A328EG16", "UniRef:UniRef50_A0A328EG16", "UniRef:UniRef90_A0A328EG16" ], "nt": "ATGGTAGCAGAAATCATCCCAAGCCAAGAGGAAGGCATCGTCAGCTTTTTAGCCTTTTTCAATGAAATAGACAAACACTTTGATAAAGTCCTAAACGAGGAGGGCTTCTCCCCCTACAACGAAAAACTCAAAAAAATCGCCGACGGCAACTATACCATTTCTAGCTTTGTCAGAAAACATCTCTATCAGCTGAGAAATTTTGGTGAATTAAGAAACTTTATCACGCATGGAATCAAATCCAACGGCGAAACCTATGCCGTGCCTACCTCCTCCGCTATCGAAAAAATCAAAACCTATGCCCAAAAGATCACCGAGCCAGCAAAAGTCCTCGAATTTTTCCAAAAACCCGTCTTTACAGCCAAAAAATCTGATCTCCTGAGTGAGATCATTCCTTTGATGAAAGGGAAAAACTATACCAATATCCCGATCTACGACGACCAAGGAAATTTCGCGGGCATCCTCAGTGAATCCAGACTCTTATACCGAATCGCAGACCTCCTTATGAGTGATGATTACCTCAATCTCAAACTCATGAAAGTCGACCACATCCAGCTCAACCGTGGACGAAAAGATTACCTCTTCATCTCGAGCAACACCACGATTTTTGAGGTAAACGATCTCTTTACCCAGAGCAAAAAAAATGGCGAAAAGCTTGGCGTGCTCTTCATCACCGAAAACGGCGATCCAAACGAGGAGCTCCTCGGGATGATCTCCGCAGGCGACGCGAATCTCATTGATGAATACATTATTATGTAG", "aa": "MVAEIIPSQEEGIVSFLAFFNEIDKHFDKVLNEEGFSPYNEKLKKIADGNYTISSFVRKHLYQLRNFGELRNFITHGIKSNGETYAVPTSSAIEKIKTYAQKITEPAKVLEFFQKPVFTAKKSDLLSEIIPLMKGKNYTNIPIYDDQGNFAGILSESRLLYRIADLLMSDDYLNLKLMKVDHIQLNRGRKDYLFISSNTTIFEVNDLFTQSKKNGEKLGVLFITENGDPNEELLGMISAGDANLIDEYIIM", "aa_hexdigest": "21ef2f9d788c17b0ed3ba93df0b772c1", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000DBF4C30", "uniref100_id": "UniRef100_A0A328EG16", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBF4C30", "UniRef:UniRef100_A0A328EG16" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EG16", "uniref90_id": "UniRef90_A0A328EG16" }, "psc": { "uniref90_id": "UniRef90_A0A328EG16", "product": "CBS domain-containing protein", "uniref50_id": "UniRef50_A0A328EG16" }, "pscc": { "uniref50_id": "UniRef50_A0A328EG16", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EG16" ], "product": "CBS domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_320", "locus": "GCA003260355_00283" }, { "type": "cds", "sequence": "contig_1", "start": 142079, "stop": 142357, "strand": "+", "frame": 2, "gene": null, "product": "IS1 family transposase", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBF5BF1", "UniRef:UniRef100_A0A328EJ28", "UniRef:UniRef50_A0A328EJ28", "UniRef:UniRef90_A0A328EJ28" ], "nt": "ATGACTCAGTGTGTGAAATGCAAGAGTTCGGAGACGAGAAAGCATGGTTTTGGCAGGAATGCGACGCAGAGGTTTTTTTGCAAGCAATGTCATTCGAGCTTTACGGTCGATGGAGTCAGAGGGACCTATTCTCCTGAGTTTAAGGAGTCAGTCGTGGAATCCTACTGCCACCAGAGGCATACAGCACAGGATGTGACTGAAAAGTATGGAATCTCGACGAGGACGCTGGTTAAATGGAAGAAAGAACATCAAACCCATTGTGATTGTGGGGAAAAATAA", "aa": "MTQCVKCKSSETRKHGFGRNATQRFFCKQCHSSFTVDGVRGTYSPEFKESVVESYCHQRHTAQDVTEKYGISTRTLVKWKKEHQTHCDCGEK", "aa_hexdigest": "3d4431dcd5133db4f06780d67dd747af", "start_type": "ATG", "rbs_motif": "TTATT", "ups": { "uniparc_id": "UPI000DBF5BF1", "uniref100_id": "UniRef100_A0A328EJ28", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBF5BF1", "UniRef:UniRef100_A0A328EJ28" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EJ28", "uniref90_id": "UniRef90_A0A328EJ28" }, "psc": { "uniref90_id": "UniRef90_A0A328EJ28", "product": "IS1 family transposase", "uniref50_id": "UniRef50_A0A328EJ28" }, "pscc": { "uniref50_id": "UniRef50_A0A328EJ28", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EJ28" ], "product": "IS1 family transposase" }, "genes": [], "id": "NKOCPLAKPK_321", "locus": "GCA003260355_00284" }, { "type": "cds", "sequence": "contig_1", "start": 142386, "stop": 142763, "strand": "+", "frame": 3, "gene": null, "product": "Nicotianamine synthase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EJ96", "UniRef:UniRef90_A0A328EJ96" ], "nt": "ATGGAAAGATTGAAAGAAAATCAGGTTTTTTCTTTTTGTTCTCGTGCGAAGAAATTGGCTAGTTATGAACCATCGGAGGAAGTGAATGCGTTTTTTTCAGAGCTATGTCAGTTTGCTAGGGACGAAACAGATTATGAGCTGGATTGCGATCCGATCTTTGTTTCTGATGAAGTGAAAGGGTTACAGGAGATCTGTGCGGTTGCCGAAGGGGAAATGGAGAAGTTTTGGTCAAAGAAGATTCAATTTGCTCAGGATCCGCAGAAGATGCTCAAGAGCTTCATCTATTATCAGAACTATGAGGATTTAACAGGTCTGGAATATGCCTTGCTTGCTCGTCAGATCGGGTTTTTGCAGCAGGTTTTGTTTGTAGGGAGCTGA", "aa": "MERLKENQVFSFCSRAKKLASYEPSEEVNAFFSELCQFARDETDYELDCDPIFVSDEVKGLQEICAVAEGEMEKFWSKKIQFAQDPQKMLKSFIYYQNYEDLTGLEYALLARQIGFLQQVLFVGS", "aa_hexdigest": "ce4e9dc0ce495467d8b3be33c8b5f076", "start_type": "ATG", "rbs_motif": "TTATT", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 142386, "stop": 143066, "observations": { "mutation": [ 142761, 142926 ], "stop": [ 142761, 142926 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EJ96", "query_cov": 1.0, "subject_cov": 0.4734848484848485, "identity": 1.0, "score": 245.0, "evalue": 2.49e-80, "gene_start": 1, "gene_end": 125, "reference_start": 1, "reference_end": 125, "reference_sequence": "MERLKENQVFSFCSRAKKLASYEPSEEVNAFFSELCQFARDETDYELDCDPIFVSDEVKGLQEICAVAEGEMEKFWSKKIQFAQDPQKMLKSFIYYQNYEDLTGLEYALLARQIGFLQQVLFVGSGALPLTAIILAQKWGVRSVLIEKDEEAVDLSRGLIEALGLQEKIAVVASDFLDYRGSETEYDAVILASLLFTSGDAEKLVDHLTRCVKFRACLVRTARGMRQLLYKKVNEKLLEKYMKLELLVHPQNHILNSILLYSHV" }, "paralog": false, "identity": 0.9911504424778761, "score": 438.0, "evalue": 1.36e-158, "description": "Internal stop codon at 142761, 142926. Nonsense mutation around 142761, 142926.", "psc": { "uniref90_id": "UniRef90_A0A328EJ96", "product": "Nicotianamine synthase", "uniref50_id": "UniRef50_A0A328EJ96", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EJ96", "UniRef:UniRef50_A0A328EJ96" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EJ96", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EJ96" ], "product": "Nicotianamine synthase" } }, "id": "NKOCPLAKPK_322", "locus": "GCA003260355_00285" }, { "type": "cds", "sequence": "contig_1", "start": 142767, "stop": 142928, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCCTTGACTGCGATTATTTTGGCTCAGAAGTGGGGAGTCCGTTCTGTTTTGATAGAGAAAGATGAAGAAGCTGTAGATCTTTCTCGTGGATTGATTGAAGCACTTGGGTTACAGGAGAAAATAGCGGTTGTAGCGTCAGATTTCCTTGATTATAGGTGA", "aa": "MPLTAIILAQKWGVRSVLIEKDEEAVDLSRGLIEALGLQEKIAVVASDFLDYR", "aa_hexdigest": "8cd0c21c9423918a2854795906070791", "start_type": "TTG", "rbs_motif": "TTGTA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5884.796299999999, "isoelectric_point": 4.606538963317871 }, "id": "NKOCPLAKPK_323", "locus": "GCA003260355_00286" }, { "type": "cds", "sequence": "contig_1", "start": 142950, "stop": 143180, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATTCTGGCTTCTTTGCTTTTTACCTCTGGAGATGCTGAGAAGCTCGTTGATCATCTGACTCGTTGCGTGAAGTTTAGAGCTTGTCTGGTAAGGACGGCGAGAGGAATGAGGCAACTTTTATACAAAAAGGTAAATGAAAAACTTTTAGAAAAATATATGAAGCTAGAACTTTTGGTTCATCCACAAAATCATATCTTAAATTCGATACTTTTATATTCTCATGTATAA", "aa": "MILASLLFTSGDAEKLVDHLTRCVKFRACLVRTARGMRQLLYKKVNEKLLEKYMKLELLVHPQNHILNSILLYSHV", "aa_hexdigest": "95d8bf068700d07e2aec8c0ccbecbf22", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8865.575799999995, "isoelectric_point": 9.695158576965333 }, "id": "NKOCPLAKPK_324", "locus": "GCA003260355_00287" }, { "type": "cds", "sequence": "contig_1", "start": 143186, "stop": 143308, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAGAAGTGAAACTTAATGCGCATTTACCTTTGCGTGAACAAGAAAAACAAAACACTATCGTAAATAAAATTTCTGAAAATTCAGAGGCTACAGGGAATGAAGTTTTTTTGATTTTATGA", "aa": "MSEVKLNAHLPLREQEKQNTIVNKISENSEATGNEVFLIL", "aa_hexdigest": "d3185286a6fe59695eba4c5859349bc4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4538.098199999999, "isoelectric_point": 5.098309898376464 }, "id": "NKOCPLAKPK_325", "locus": "GCA003260355_00288" }, { "type": "cds", "sequence": "contig_1", "start": 143492, "stop": 144178, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02492.25" ], "nt": "TTGAAAAGTGCTTTGGAGGATTTGAAAGGTAGTAATGAAAAAATTTTGATTGAACCTTCTGGAATCGCTGATGGAAAAGCTATTAAGAGAGTTATTGAGGAGTTAGGCATGCAGGGGAGAGTAATTACCTTGGTGAATGCTCAGTCTTTCCCAACCATGTCGAAAGCTGAAGAACAGATTGCTTCTACACAGCTTTCTTTGGCGAATGTTATTGCGCTTACGCATCTTCCTGCTGATAAAGAAAAGAATCAAGAATTAAGAGCTCAGTTGTCTCAGCAATATCCTCATCAGCCGGTTATTGAAGCGCCTTATACAACCAATAGTGAGGATCTTATGAAAGATGATAGTTCGTATGGAGCTATTGTAAATCTTATGAGAAATCATTCTCACAATAGTCATGCTGAGCATGAACACGAGCATGATCACCACGAACACGAGCATGGTCACCATGAACACGAGCATGATCACCACGAACACGAGCATGATCATCACGAACACGAGCATGATCATCATGAACACGAGCATGATCATCATGAACACGAGCATGATCATCATGAACATGGGCACCATCATCATGAGCATTCAAAATTTTTCACAAAGAGTATTGCTTTGTCTGAAGAGACTTCATTAGACCAGTTGAAAGACCTTATTCATCAGCTTGGGCAAGATGCGCTTCGTATAAAGTGA", "aa": "MKSALEDLKGSNEKILIEPSGIADGKAIKRVIEELGMQGRVITLVNAQSFPTMSKAEEQIASTQLSLANVIALTHLPADKEKNQELRAQLSQQYPHQPVIEAPYTTNSEDLMKDDSSYGAIVNLMRNHSHNSHAEHEHEHDHHEHEHGHHEHEHDHHEHEHDHHEHEHDHHEHEHDHHEHEHDHHEHGHHHHEHSKFFTKSIALSEETSLDQLKDLIHQLGQDALRIK", "aa_hexdigest": "9929fc76889cfe10e52cbb58ed03935b", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02492.25", "name": "cobW", "length": 104, "aa_cov": 0.42543859649122806, "hmm_cov": 1.0, "evalue": 1.6060034200571494e-06, "score": 24.267169952392578, "start": 4, "stop": 100 } ], "seq_stats": { "molecular_weight": 26392.31410000006, "isoelectric_point": 5.76855525970459 }, "id": "NKOCPLAKPK_326", "locus": "GCA003260355_00289" }, { "type": "cds", "sequence": "contig_1", "start": 144248, "stop": 144544, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACGACACTTCAGCCGCATCTTAATGTTATTACTCAACGTTCTTTATCTCCAGAATTGATAGCTATGATTTCTTCGGGTTCAACTTCTTCTGAAAAACTTTCTTATGTACCAGAAAAGAAAATTTACTCTCCAATAGAGGTTTCTGCTGCGGTTTCTGTTCTTTTGAATCAATATAAGGACTATATGAATATGTATAATGAAAAATTAGCTTTAGAGACTGAATATGCCAAAAATCAGGATTCTAAATTGGCTCAAGAGATTCAGCAGCTTGATATTGCTCTGGACAAGCTTTGA", "aa": "MTTLQPHLNVITQRSLSPELIAMISSGSTSSEKLSYVPEKKIYSPIEVSAAVSVLLNQYKDYMNMYNEKLALETEYAKNQDSKLAQEIQQLDIALDKL", "aa_hexdigest": "531a1a916d59cfa8f1fad219efe1ae73", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11053.532399999993, "isoelectric_point": 5.021690940856933 }, "id": "NKOCPLAKPK_327", "locus": "GCA003260355_00290" }, { "type": "cds", "sequence": "contig_1", "start": 145339, "stop": 145554, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAAAAGCCACACTTATCAGCAAAACATTCCTCAATGACCAAGTAATAGAACTCAAACTAAAAACAGAAAAACTTGCAACTCAGCCTGGACAACGGCTTTTTATCCATTATTTAGAGAGCGAAACACCGCTCAAGAGAGCTTACTCTATCGCAAATGAGGAACAGGAAGGTGATTTCAGTATTTTTACCTTTTTGATAAAACTTATTCCTTGA", "aa": "MQKATLISKTFLNDQVIELKLKTEKLATQPGQRLFIHYLESETPLKRAYSIANEEQEGDFSIFTFLIKLIP", "aa_hexdigest": "31617923079d43145241d3ad798e9596", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8228.470099999993, "isoelectric_point": 6.559640312194825 }, "id": "NKOCPLAKPK_328", "locus": "GCA003260355_00291" }, { "type": "cds", "sequence": "contig_1", "start": 145701, "stop": 145889, "strand": "-", "frame": 2, "gene": null, "product": "Transposase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EG02", "UniRef:UniRef90_A0A328EG02" ], "nt": "ATGAATATAAAAACAAAACGTAGACCACCTGAGAAAAAAACCCTCTACGAATGGATAGAGAGGCTCTCCTTTGATGAGGGGGAGAAATTTCTTGTATTCTGTAGAAAATATTATTATCTTTATCCAGAATATACCGTGTATAGGACAAAGTTTGAACAGCTCTATCGAAAGTTTCTGCGTCAAAAATGA", "aa": "MNIKTKRRPPEKKTLYEWIERLSFDEGEKFLVFCRKYYYLYPEYTVYRTKFEQLYRKFLRQK", "aa_hexdigest": "d00785943bbb7c75c983d5172a926613", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 145602, "stop": 145889, "observations": { "mutation": [ 145643, 145673, 145703 ], "stop": [ 145643, 145673, 145703 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EG02", "query_cov": 1.0, "subject_cov": 0.6526315789473685, "identity": 1.0, "score": 129.0, "evalue": 8.03e-38, "gene_start": 1, "gene_end": 62, "reference_start": 1, "reference_end": 62, "reference_sequence": "MNIKTKRRPPEKKTLYEWIERLSFDEGEKFLVFCRKYYYLYPEYTVYRTKFEQLYRKFLRQKGQSCPLHPRYGINQKIRTIRGVRIFLLVQKLAK" }, "paralog": false, "identity": 0.968421052631579, "score": 189.0, "evalue": 6.18e-64, "description": "Internal stop codon at 145643, 145673, 145703. Nonsense mutation around 145643, 145673, 145703.", "psc": { "uniref90_id": "UniRef90_A0A328EG02", "product": "Transposase", "uniref50_id": "UniRef50_A0A328EG02", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EG02", "UniRef:UniRef50_A0A328EG02" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EG02", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EG02" ], "product": "Transposase" } }, "id": "NKOCPLAKPK_329", "locus": "GCA003260355_00292" }, { "type": "cds", "sequence": "contig_1", "start": 146031, "stop": 146528, "strand": "-", "frame": 2, "gene": "tatD", "product": "Hydrolase TatD", "db_xrefs": [ "EC:3.1.21.-", "KEGG:K03424", "SO:0001217", "UniRef:UniRef50_A0A328EJA0", "UniRef:UniRef90_A0A328EJA0" ], "nt": "GTGCCACTTACTGGAATTCTTGATGAAAAAATCAAAGAGCTCAAAAATCAGATTATAGATCATCAGGAAGAGGTAGTAGCGATTGGAGAATGCGGGATTGATCTTCATTATGATACTGAAGGGAAAACGCTTTCTCTCCAGCAAAAAGTCTTTGAAGCTCAGTGTGAACTCGCAAGAGAGCTAGCATTACCCATTGTAATCCACTCAAGAGACGCTTTTGAAGTAACTGTTAAAATCTTAAAAAATTACCAAAATCTGACAATCTATTTCCATTGTCGAGGATACGGACCTGCAGAACTCAAAGAACTTCTAGAGCTTTTCCCTAACCTTTACATTGGTATCTGTGGAAATGTAAGTTATCCTCGTGCAGAAACACTCAGGGAAAGCATCTTTTGCATTCCTAAAGATCGTCTTCTCATAGAAACCGACGCTCCTTACCTCGCTCCAAAAGAATTTCGCGGAAAATTAAACATGCCTGAAAGGGTGATTCAGATTTGA", "aa": "MPLTGILDEKIKELKNQIIDHQEEVVAIGECGIDLHYDTEGKTLSLQQKVFEAQCELARELALPIVIHSRDAFEVTVKILKNYQNLTIYFHCRGYGPAELKELLELFPNLYIGICGNVSYPRAETLRESIFCIPKDRLLIETDAPYLAPKEFRGKLNMPERVIQI", "aa_hexdigest": "9b9f3f5271c47e0e5ee50858686ed70a", "start_type": "GTG", "rbs_motif": null, "genes": [ "tatD" ], "truncated": "both", "pseudogene": { "start": 145944, "stop": 146750, "observations": { "mutation": [ 146018, 146033, 146639, 146663 ], "stop": [ 146018, 146033, 146639, 146663 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EJA0", "query_cov": 1.0, "subject_cov": 0.6156716417910447, "identity": 0.9940000000000001, "score": 335.0, "evalue": 7.47e-115, "gene_start": 1, "gene_end": 165, "reference_start": 75, "reference_end": 239, "reference_sequence": "MEFYDSHTHLNHEELFPNWKTLLQSFIEKGGKGLVNAGANQNYNERGLLIAQEAKKLFPDCRVKCCLGFHPCDVVPLTGILDEKIKELKNQIIDHQEEVVAIGECGIDLHYDTEGKTLSLQQKVFEAQCELARELALPIVIHSRDAFEVTVKILKNYQNLTIYFHCRGYGPAELKELLELFPNLYIGICGNVSYPRAETLRESIFCIPKDRLLIETDAPYLAPKEFRGKLNMPERVIQIGSFIAGLVGQKEDLFREQIKQNFFNIYKK" }, "paralog": false, "identity": 0.9850746268656716, "score": 536.0, "evalue": 1.56e-196, "description": "Internal stop codon at 146018, 146033, 146639, 146663. Nonsense mutation around 146018, 146033, 146639, 146663.", "psc": { "uniref90_id": "UniRef90_A0A328EJA0", "product": "Hydrolase TatD", "ec_ids": [ "3.1.21.-" ], "uniref50_id": "UniRef50_A0A328EJA0", "kegg_orthology_id": "K03424", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EJA0", "EC:3.1.21.-", "UniRef:UniRef50_A0A328EJA0", "KEGG:K03424" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EJA0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EJA0" ], "product": "Hydrolase TatD" } }, "id": "NKOCPLAKPK_330", "locus": "GCA003260355_00293" }, { "type": "cds", "sequence": "contig_1", "start": 147213, "stop": 147551, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATCGCTTCAAATCACAAGTAATGATGCCATGGTTGAGTATCAGCCGAGGAGAGAACGACTACTTTACCTTTCCGAGCAAAGATTCTTATGTTCAGCTCAATTGGAGCCAAAATGTTAGTGGAGCAGTGAAAAATATCATTGCTGCCGAATACGAAGGAAAGGAGTATCTACCAAAATACTATTATGCAGGAGGCTATAGCTTACAGGATCTTGCAGGTAACACTTCAGGGACTAAAATCGGAAGCAATAACGGAGAAGAAAGAGACAGCTATCTTTTTTTTGATCTTCCTATTCAGCAAGAACACCAGCTTAAAAATCTGAATTTCAAACTTTGA", "aa": "MYRFKSQVMMPWLSISRGENDYFTFPSKDSYVQLNWSQNVSGAVKNIIAAEYEGKEYLPKYYYAGGYSLQDLAGNTSGTKIGSNNGEERDSYLFFDLPIQQEHQLKNLNFKL", "aa_hexdigest": "06bcfc5d30bf98569d62fc639993caa8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12945.29749999999, "isoelectric_point": 5.758210563659667 }, "id": "NKOCPLAKPK_331", "locus": "GCA003260355_00294" }, { "type": "cds", "sequence": "contig_1", "start": 147564, "stop": 147725, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAAACATCTACGGACAGCAAAAAAGCGACCTCAAAAAAGCCCTTGAGGACAATAATACCAAAGAGGTGGAAAGAATTACTCAAGATTGGGAACTTATCCGTCAAGTTGGGAATCATCTTTACAAAGTAAAAGGGACACAAAACCAGAAAGAAGTCTGA", "aa": "MQNIYGQQKSDLKKALEDNNTKEVERITQDWELIRQVGNHLYKVKGTQNQKEV", "aa_hexdigest": "a2f2795c7b2373215cd929a446100fa3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6287.982000000002, "isoelectric_point": 8.03052043914795 }, "id": "NKOCPLAKPK_332", "locus": "GCA003260355_00295" }, { "type": "cds", "sequence": "contig_1", "start": 147766, "stop": 147984, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTCGTATCTAGTTGGGTGATCAATCCAGAGATAATGGCCTGTCTCTTGCCAGTTAGTGATTCATTGGCAAAGCTCAGATTCCAAGCAAAAGCTCCGATTAGAGTGAGGATCAAGAGTTTTTTCATTAAGATTGTGAGGAAAAATAAAAAAAGAATAGTGGTGTTGTATGCTTTTTGGGGTAAAAAGCAACTCAAAATTTATGGAGAAGGCGTCTAA", "aa": "MFVSSWVINPEIMACLLPVSDSLAKLRFQAKAPIRVRIKSFFIKIVRKNKKRIVVLYAFWGKKQLKIYGEGV", "aa_hexdigest": "71ed1cf8ec9b0d4e3f571f1b81f10d8e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8369.145799999998, "isoelectric_point": 10.650708961486817 }, "id": "NKOCPLAKPK_333", "locus": "GCA003260355_00296" }, { "type": "cds", "sequence": "contig_1", "start": 148262, "stop": 148444, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTCTATATCTCCATCTTTGGCGAATACTTCATTTTCCTTAAGGTAGGAAAATCAACTAAAATCTCTTGTAAATTTGAAGTCTTTGGTTATTGGCTGAATTTTTTCTCTAAATTGGTAGATGTTCTGTTGTTTCCTTTCTTTTTTTCTATTATTTGGTGGGAGGTATTGATAATATTCTAA", "aa": "MLYISIFGEYFIFLKVGKSTKISCKFEVFGYWLNFFSKLVDVLLFPFFFSIIWWEVLIIF", "aa_hexdigest": "66fca45ffa39629d6aa5afc8c713be78", "start_type": "ATG", "rbs_motif": "TTTTT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7321.761099999994, "isoelectric_point": 7.855359840393066 }, "id": "NKOCPLAKPK_334", "locus": "GCA003260355_00297" }, { "type": "cds", "sequence": "contig_1", "start": 148478, "stop": 148825, "strand": "-", "frame": 3, "gene": null, "product": "Succinylglutamate desuccinylase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2G6CC34", "UniRef:UniRef90_A0A328EFF1" ], "nt": "ATGAATCTCCTTGCCTATGAGGAACACAGAAGGTTCATTGATGATAACATGAACCGTATTTCTAATCAAGCGTATAAATCGACAAGCTATGAACATCAAAGATTAGGTGAACTGAAACCAATACTTAGAGAAATGGATATTACTTTAGATATTCATAGTTTTTCAAATAGTCATGATATTGTAGCAATTTGCGATATCCAGAATAGAGAAAAAGCAAAGCAGATATTCACCACAGAGACAATTTTGGTCGATAATTTAGAACAATCAGGAAGTATTATAGGATATTGCACCAGAAAACAAAAAGAAGCCTACGGAATCGAATGCGGACTGCACGATGATCCAGAGTGA", "aa": "MNLLAYEEHRRFIDDNMNRISNQAYKSTSYEHQRLGELKPILREMDITLDIHSFSNSHDIVAICDIQNREKAKQIFTTETILVDNLEQSGSIIGYCTRKQKEAYGIECGLHDDPE", "aa_hexdigest": "e07d243875fd1920bf7ce92e5bdc0784", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 148175, "stop": 149029, "observations": { "mutation": [ 148249, 148318, 148480, 148921, 148954, 148963 ], "stop": [ 148249, 148318, 148480, 148921, 148954, 148963 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EFF1", "query_cov": 1.0, "subject_cov": 0.40069686411149824, "identity": 1.0, "score": 236.0, "evalue": 2.13e-76, "gene_start": 1, "gene_end": 115, "reference_start": 69, "reference_end": 183, "reference_sequence": "MITNPFLTNQYKNISGITLIDSGNPGKNLGLLAITHGNETVGLKVFAYLKQEILKKHPLQQGKLFLIAMNLLAYEEHRRFIDDNMNRISNQAYKSTSYEHQRLGELKPILREMDITLDIHSFSNSHDIVAICDIQNREKAKQIFTTETILVDNLEQSGSIIGYCTRKQKEAYGIECGLHDDPEGFERGKEEILNFLEYYQYLPPNNRKKERKQQNIYQFREKIQPITKDFKFTRDFSGFSYLKENEVFAKDGDIEHKNTLGKNIYLGIPAKEPRVNDSAGFLFKKLE" }, "paralog": false, "identity": 0.9788732394366197, "score": 555.0, "evalue": 1.5e-204, "description": "Internal stop codon at 148249, 148318, 148480, 148921, 148954, 148963. Nonsense mutation around 148249, 148318, 148480, 148921, 148954, 148963.", "psc": { "uniref90_id": "UniRef90_A0A328EFF1", "product": "Succinylglutamate desuccinylase", "uniref50_id": "UniRef50_A0A2G6CC34", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EFF1", "UniRef:UniRef50_A0A2G6CC34" ] }, "pscc": { "uniref50_id": "UniRef50_A0A2G6CC34", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2G6CC34" ], "product": "Succinylglutamate desuccinylase" } }, "id": "NKOCPLAKPK_335", "locus": "GCA003260355_00298" }, { "type": "cds", "sequence": "contig_1", "start": 149430, "stop": 150170, "strand": "+", "frame": 3, "gene": "rplI", "product": "50S ribosomal protein L9", "db_xrefs": [ "GO:0003735", "GO:0005840", "GO:0006412", "KEGG:K02939", "SO:0001217", "UniRef:UniRef50_A0A7W4ECZ4", "UniRef:UniRef90_A0A660MXR1" ], "nt": "ATGACGAGCGGGAGAGTGTCAGAACGTCAGTTTTCTAAAATCTCATTGCATTTTATTCCGAAATCTGTATATTCTTTCACTGAATCTTATTTTACTTCGTTAAAGAAGCTTTTTATAATGCCAAGAAGAACATCTAAAAACAGAAACATTGAGGTTATTTTGCTTCAGGATAACAAACATCTCGGGGAAAAATATGAAATTATTGGAGTGAAGCCTATCTTTGCTAGGAATGTGCTTTTCCCACAGCAGATTGCTGTGCTTGCAGACAAGGCAAACAGGAATAAATATGAGCAAAAGATGCAGGCGGCTATAGCTGCAAGAGCGAAAAAAGCTAGTGGGCTTGAAGAACTCTTTGCTAAAATGACAGAAAATGGAGGAGTAAAGCTTTTGAGAAAGGCTAATAAAGGAAAAACGCTCTATGCTAAGGTAGGAGAAACTGATATTACTGAGGCAATCAAGGAGGCTTATGGAGTGGAGGTAGAACCTCACTATCTTAAGCTCAAGAAAAAGCTCAATGAAGTAGGAGTTTTTGTGGTTCCTTTTATTTACAAGGATCTTAAGAGAGAGATTTCTGTGACGATTGAGGCTGAAGGTGGAGTTCAAGAAGAAGTTAAGGTAGAAGTTGAGGCAGTGGAAGCAGCTCCAGAAAAGACTAAGGAAGAACTTAAAGCTGAAAGAGAAGCAAAAAGAGCTGCTGAGAAAGCAGATAAGATTGCAAAGCTCAAGGAGAAATATAAATAA", "aa": "MTSGRVSERQFSKISLHFIPKSVYSFTESYFTSLKKLFIMPRRTSKNRNIEVILLQDNKHLGEKYEIIGVKPIFARNVLFPQQIAVLADKANRNKYEQKMQAAIAARAKKASGLEELFAKMTENGGVKLLRKANKGKTLYAKVGETDITEAIKEAYGVEVEPHYLKLKKKLNEVGVFVVPFIYKDLKREISVTIEAEGGVQEEVKVEVEAVEAAPEKTKEELKAEREAKRAAEKADKIAKLKEKYK", "aa_hexdigest": "55d50b757926e0e7c6c8f536a12d5738", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A660MXR1", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.9229999999999999, "score": 397.0, "evalue": 2.74e-138, "valid": true, "gene": "rplI", "product": "50S ribosomal protein L9", "uniref50_id": "UniRef50_A0A7W4ECZ4", "kegg_orthology_id": "K02939", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_A0A7W4ECZ4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7W4ECZ4" ], "product": "Large ribosomal subunit protein bL9" }, "genes": [ "rplI" ], "id": "NKOCPLAKPK_336", "locus": "GCA003260355_00299" }, { "type": "cds", "sequence": "contig_1", "start": 150558, "stop": 151346, "strand": "+", "frame": 3, "gene": "nrdG", "product": "anaerobic ribonucleoside-triphosphate reductase activating protein", "db_xrefs": [ "EC:1.97.1.4", "GO:0043365", "GO:0051539", "KEGG:K04068", "SO:0001217", "UniRef:UniRef50_A0A7W4HGV2", "UniRef:UniRef90_A0A328EJ35" ], "nt": "GTGAGTGTGAAATGTCGATGTGCTTTGGATCATCCGCAGTGCAGAAGAGAGTTTGTTTTCAAAACGGAAGGTAAGGAAGTGTTTGTGATGGTGCGTTGGAGGCAAGCTGAATCTTGGAAATTTATCAGACTTTTGAGGCTTTTTTTATTTCTTTCTAAAGAGTTAAGAAAAATTATGAACTACGGAACGATCAAAACCATGGATACGGCGAATGGTGAGGGGCTTAGGGTCTCTCTTTTTGTGTCTGGGTGTCATCATGCTTGTCCGGGGTGTTTTAATCCTGAGGCGTGGAATTTTGCTTTTGGGAAGCCCTTTACACAAGAAACGATTGATGAGATTATCCAAGAGTTGAGTGCTGATTACATCTCGGGATTGTCGCTGCTCGGGGGAGAGCCTTTGGCGCCGGAGAATCAAGAGCAGGTTTGGAATTTGGTTAGGCAAGTTAGAGAGCAGCTTCCGCACAAAACGATATGGTGTTACTCAGGGTTTACTTACGAATTTATCACGGAGGTGATGTTTCCGAATTTACCGTTTACGTATCAGATTTTGTCGCATCTGGATGTGCTGATTGAGGGAAGGTTTGTGCAGCAGCTCGTAGATCTCACGATCAAGTTTAGGGGGTCTAGGAATCAGAGAGTGCTGGACGTTCCGCTTTCTCTGGAAAAGGGGATGCCGATTTGGAGCACTGCGGTCGATGACCAGAAGGCTTATGAGTATTATCCGAGCCCTGAGAAGGAACTCTTTACTGCAAGGATCGAGAATTTCCTTCACAAGGTGACAGTGGAGTAG", "aa": "MSVKCRCALDHPQCRREFVFKTEGKEVFVMVRWRQAESWKFIRLLRLFLFLSKELRKIMNYGTIKTMDTANGEGLRVSLFVSGCHHACPGCFNPEAWNFAFGKPFTQETIDEIIQELSADYISGLSLLGGEPLAPENQEQVWNLVRQVREQLPHKTIWCYSGFTYEFITEVMFPNLPFTYQILSHLDVLIEGRFVQQLVDLTIKFRGSRNQRVLDVPLSLEKGMPIWSTAVDDQKAYEYYPSPEKELFTARIENFLHKVTVE", "aa_hexdigest": "51e233751518529d3921c208f3453f17", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A328EJ35", "query_cov": 0.8893129770992366, "subject_cov": 1.0, "identity": 1.0, "score": 479.0, "evalue": 5.58e-171, "valid": true, "gene": "nrdG", "product": "anaerobic ribonucleoside-triphosphate reductase activating protein", "ec_ids": [ "1.97.1.4" ], "uniref50_id": "UniRef50_A0A7W4HGV2", "kegg_orthology_id": "K04068", "go_ids": [ "GO:0043365", "GO:0051539" ] }, "pscc": { "uniref50_id": "UniRef50_A0A7W4HGV2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7W4HGV2" ], "product": "Anaerobic ribonucleoside-triphosphate reductase-activating protein" }, "genes": [ "nrdG" ], "id": "NKOCPLAKPK_337", "locus": "GCA003260355_00300" }, { "type": "cds", "sequence": "contig_1", "start": 151452, "stop": 151727, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTTATATGGGAATCTATTCCAACCGCCCCGGCACTTTCGCCGATAGAAAATACCCAGACGATATCCCTTATTCCGTCAAACACGAGAGACGATCCCGCCTCAACAAACTCCTCTACCAAATCTCGACCGAAAATAACCAACAGGAAATCGGGAAAATCAGAACAATGATGATAAACGAAATCGGTCCCAACGGCGACCTTTACGGCTATACCGATAATATGAAACAAATCACCATCAAAGCAAACGCAAAGACCAAAGGCATAAAAATCTGA", "aa": "MIYMGIYSNRPGTFADRKYPDDIPYSVKHERRSRLNKLLYQISTENNQQEIGKIRTMMINEIGPNGDLYGYTDNMKQITIKANAKTKGIKI", "aa_hexdigest": "fcec6fadd59e24869a87bc461c4d35e3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10574.055099999987, "isoelectric_point": 9.654479026794434 }, "id": "NKOCPLAKPK_338", "locus": "GCA003260355_00301" }, { "type": "cds", "sequence": "contig_1", "start": 151785, "stop": 152360, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF04055.27" ], "nt": "ATGCTCCAAAAACTCGGCTACAACATCCACTATGATCAAGAATTGACCAATGAATACTCCACTATCATCCCAGCAAACATCAATTCCAGTATGAACGCCCACGACCAAACCGCCTACATCCCTATCTCAAGCGGCTGCAACCAGTTTTGTGCCTATTGTATTGTGCCATATGCGCGTGGACTGGAGAAATGGTTCCCGGTAGAGCAAATCATCCACGAAGCCAAAACCCACCTCGCTCACGGCGTCAAAGAAATCTCACTCCTCGGACAAATCGTCAATAAACACCCAGATTTTTTGACTATTGTAAAAGAGCTTTTAAAACTAGAGGGCTTAGAATGGCTCAGATACACTTCCCCTTACCCGACCTACTATTCTGACGAGCTGCTCGCACTGCACCAAAACGAGCAAAAACTCTGCCCTCATATCCATATTCCATTCCAGTCAGGCTCTACTCCAGTGTTGAAAAAAATGTTCCGCGGCTATAACGCAGAGCAGTGCTATACTTTTATAGATAAAATTAGGTCCCTTAGCAGACCAATCTCTATCACAACCGATATAATCCTCGGCTTTCCTTGA", "aa": "MLQKLGYNIHYDQELTNEYSTIIPANINSSMNAHDQTAYIPISSGCNQFCAYCIVPYARGLEKWFPVEQIIHEAKTHLAHGVKEISLLGQIVNKHPDFLTIVKELLKLEGLEWLRYTSPYPTYYSDELLALHQNEQKLCPHIHIPFQSGSTPVLKKMFRGYNAEQCYTFIDKIRSLSRPISITTDIILGFP", "aa_hexdigest": "f016746dd351087a97b8f168f2152f20", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF04055.27", "name": "Radical_SAM", "length": 157, "aa_cov": 0.7958115183246073, "hmm_cov": 0.9681528662420382, "evalue": 2.2550017332650844e-20, "score": 69.9017562866211, "start": 40, "stop": 191 } ], "seq_stats": { "molecular_weight": 21945.000900000014, "isoelectric_point": 6.489444160461425 }, "id": "NKOCPLAKPK_339", "locus": "GCA003260355_00302" }, { "type": "cds", "sequence": "contig_1", "start": 152562, "stop": 152855, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATTCCACGTGATTACCTTGGGTTGCCAAATGAACTATTCCGACACCGCCAGAATCAAATCTGTTCTCAAAAATTGTGGTTTTGATCCTGTAGAAACGATCGCCGAGGCAGATATCGTTATTTTTGATACTTGCTCCGTCCGCCAAAAGTCAGAGGACAAAGTCACAGGTAAACTCAAGGAAATCCCGCTTACCAAAAAAGTGCGAATCACCGGCTGTATGATCCAGCATACCATGAGAAACCAGAAAGTCAGCACTGCTGCCAAACTTCCAGACCAGCTCAAAACCTGA", "aa": "MKFHVITLGCQMNYSDTARIKSVLKNCGFDPVETIAEADIVIFDTCSVRQKSEDKVTGKLKEIPLTKKVRITGCMIQHTMRNQKVSTAAKLPDQLKT", "aa_hexdigest": "6ea1d7b39c17a2530c1eac0ce929f2c5", "start_type": "ATG", "rbs_motif": "TTATT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10885.708899999989, "isoelectric_point": 9.327173042297364 }, "id": "NKOCPLAKPK_340", "locus": "GCA003260355_00303" }, { "type": "cds", "sequence": "contig_1", "start": 153166, "stop": 153627, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EG03", "UniRef:UniRef90_A0A328EG03" ], "nt": "ATGAAAACATCCACAAAAACTCAGCTCATCCAAGCCTGCTTCAACAAAACCGTCCTCAAGAGACGAACAAAACTCTTGACCACACTCCTTGCAATCATTGGACTCTTAAGTCTGATCCTCATCGCTTCAAGTAATTACTTAAAATACCATATGTATAGACTAGGCTTCGAAAGTATTCTACGTATCAAGGATAACATCGTTGAAAAAGTCCTTGAAAAAGAACAAGGTTTCAACCTCGCCACCACAGAAAGCATCCCTTACGACACGGAAATTCATGTGCCTCAGGGAAGAATCGATGTTTCAATGCCAACCTTCCTCTATGCGAGTGATCGAAACTTCGCACTTGAAGTTTCCGCTCTCAGTGGAAAAGCAGGGCTCAAATACCTTACCTATAATCCACGCTATGAAGGCATTCTAAACCCCATTCCTTATACTTTTCAGATCTTGATTAAACCCTTTTGA", "aa": "MKTSTKTQLIQACFNKTVLKRRTKLLTTLLAIIGLLSLILIASSNYLKYHMYRLGFESILRIKDNIVEKVLEKEQGFNLATTESIPYDTEIHVPQGRIDVSMPTFLYASDRNFALEVSALSGKAGLKYLTYNPRYEGILNPIPYTFQILIKPF", "aa_hexdigest": "de5c12acf8f0a96e7552b2ea41cbeafe", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 152953, "stop": 153627, "observations": { "mutation": [ 152997, 153021, 153060, 153168 ], "stop": [ 152997, 153021, 153060, 153168 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EG03", "query_cov": 1.0, "subject_cov": 0.6830357142857143, "identity": 1.0, "score": 293.0, "evalue": 2.82e-99, "gene_start": 1, "gene_end": 153, "reference_start": 1, "reference_end": 153, "reference_sequence": "MKTSTKTQLIQACFNKTVLKRRTKLLTTLLAIIGLLSLILIASSNYLKYHMYRLGFESILRIKDNIVEKVLEKEQGFNLATTESIPYDTEIHVPQGRIDVSMPTFLYASDRNFALEVSALSGKAGLKYLTYNPRYEGILNPIPYTFQILIKPFGLEDEKTPSCLLITKGVPGMPASTRPEQNPNNCEVIGIQPEFYDEKHLIGVSAFSKGGATVLFTKRNLRQE" }, "paralog": false, "identity": 0.9821428571428571, "score": 430.0, "evalue": 1.03e-155, "description": "Internal stop codon at 152997, 153021, 153060, 153168. Nonsense mutation around 152997, 153021, 153060, 153168.", "psc": { "uniref90_id": "UniRef90_A0A328EG03", "uniref50_id": "UniRef50_A0A328EG03", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EG03", "UniRef:UniRef50_A0A328EG03" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EG03", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EG03" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 17466.32309999999, "isoelectric_point": 9.518773078918457 }, "id": "NKOCPLAKPK_341", "locus": "GCA003260355_00304" }, { "type": "cds", "sequence": "contig_1", "start": 153696, "stop": 154679, "strand": "-", "frame": 2, "gene": null, "product": "RCK N-terminal domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EJV5", "UniRef:UniRef90_A0A328EJV5" ], "nt": "ATGGGAGGAATCACGCTTTCCACTTCTCCCTACAGATTCGAAATCGCCAGCAGAATGAAGGTGCTCAAAGACTTTTTCATCGTGGTATATTTCGTGCTCTTGGGAAGTCAGATTTCCTTTGATGGCAACCTTAATCGACCATTCATCGGAGCAGGATTGGCTGCCGTCATCTTTTTCAAACCACGAATTACAATGTGGCTGATGAAACTCATGGGTCATGTTAAAAAAAACAACTTCCTAACAGGACTTACTCTCATCCCAATGAGTGAATTTTCCTTTATCTTGATCTCAATGGGAATCTCTTCTGGAGCGATTAAAGATCAGAACCTTCTCGTGATGATCACGATTATCGGAATTACAAGTATTCTGATCTCTGGATACGCCACAGCATACAATCATATCCTCTTCAAAAAACTCGGGAAACGGCAAAAATACATCCCGGGTTCAATCTCCAGAAAATCAAAGGAAACTCTTGGAAGCCCAAATGATATCGTCCTCTTCGGCTTCGGCAAACTCGGAAGCAGTCTTTATGAGCATTTTCAAAAAAAGAAGTATTCTTTTCTAATCATTGACGAGCATCCTGCTATTATCAACCACCTCAACACGCACGAAATCAATTGCCTCTATGGAGATGCTCTCGATTTAGAGTTTTTGAATGACATTAATGTTTCAGGCACCAAAATGGTAATCTCAACGATCAGAAACGTGGACGCTAACTTCGCAATTTTAAGCTGTTTAAAAGAAAAAAAGGCTGATCTCATCGTCATTTGTATCGCAAATCACATTCACGAGGCGATCACCCTCTACGAAAAAGGTGCAGATTACGTCATTATGCCCCACTATATCGGAGCTGATCATACATCTATCATGCTGGAAGAATTTGGACTCGATCTAGAAAAATTCCTCAAAAATAAATCACTCCAAGTCGAGAACCTCAGAAAAAAAAGCAATGATCTTCTCCTCGAAGAGCTCATAAAAAAATAA", "aa": "MGGITLSTSPYRFEIASRMKVLKDFFIVVYFVLLGSQISFDGNLNRPFIGAGLAAVIFFKPRITMWLMKLMGHVKKNNFLTGLTLIPMSEFSFILISMGISSGAIKDQNLLVMITIIGITSILISGYATAYNHILFKKLGKRQKYIPGSISRKSKETLGSPNDIVLFGFGKLGSSLYEHFQKKKYSFLIIDEHPAIINHLNTHEINCLYGDALDLEFLNDINVSGTKMVISTIRNVDANFAILSCLKEKKADLIVICIANHIHEAITLYEKGADYVIMPHYIGADHTSIMLEEFGLDLEKFLKNKSLQVENLRKKSNDLLLEELIKK", "aa_hexdigest": "a639e2fc4160ba9f60355abba0f8795c", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 153696, "stop": 154979, "observations": { "mutation": [ 154688, 154832, 154964 ], "stop": [ 154688, 154832, 154964 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EJV5", "query_cov": 1.0, "subject_cov": 0.5736842105263158, "identity": 1.0, "score": 635.0, "evalue": 4.45e-226, "gene_start": 1, "gene_end": 327, "reference_start": 244, "reference_end": 570, "reference_sequence": "MDQFFLSFAVILGIVLLSLGAMKILKQPMIIGYILAGIVISFLFPGVFAESETFTQLSQVGITFLLFIIGTELNPSLIHKIGGKTVAVGVLQVIGSSILGFGIGLAFKFDIMTSLYIGTATALSSTIVILKLLGDKDDIDTTYGRLSIGTSVSQDISVMLFMSIVAAFTAMGDGDSLHVAGILILKILGIGIGLFIFSKYLLPYFTRKIAESQEFLLLFSIGRCFITSAIFQTLGFGMEIGALMGGITLSTSPYRFEIASRMKVLKDFFIVVYFVLLGSQISFDGNLNRPFIGAGLAAVIFFKPRITMWLMKLMGHVKKNNFLTGLTLIPMSEFSFILISMGISSGAIKDQNLLVMITIIGITSILISGYATAYNHILFKKLGKRQKYIPGSISRKSKETLGSPNDIVLFGFGKLGSSLYEHFQKKKYSFLIIDEHPAIINHLNTHEINCLYGDALDLEFLNDINVSGTKMVISTIRNVDANFAILSCLKEKKADLIVICIANHIHEAITLYEKGADYVIMPHYIGADHTSIMLEEFGLDLEKFLKNKSLQVENLRKKSNDLLLEELIKK" }, "paralog": false, "identity": 0.9929742388758782, "score": 820.0, "evalue": 1.98e-301, "description": "Internal stop codon at 154688, 154832, 154964. Nonsense mutation around 154688, 154832, 154964.", "psc": { "uniref90_id": "UniRef90_A0A328EJV5", "product": "RCK N-terminal domain-containing protein", "uniref50_id": "UniRef50_A0A328EJV5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EJV5", "UniRef:UniRef50_A0A328EJV5" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EJV5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EJV5" ], "product": "RCK N-terminal domain-containing protein" } }, "id": "NKOCPLAKPK_342", "locus": "GCA003260355_00305" }, { "type": "cds", "sequence": "contig_1", "start": 154830, "stop": 154934, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTTTCATGTCGATCGTTGCTGCATTTACAGCGATGGGCGACGGAGATAGCTTGCACGTTGCTGGAATCCTAATTCTCAAGATCCTCGGAATCGGAATCTGA", "aa": "MLFMSIVAAFTAMGDGDSLHVAGILILKILGIGI", "aa_hexdigest": "35f6532e28f7a459f2779e1500fc6275", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3489.2595, "isoelectric_point": 5.194140434265136 }, "id": "NKOCPLAKPK_343", "locus": "GCA003260355_00306" }, { "type": "cds", "sequence": "contig_1", "start": 154962, "stop": 155132, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATCGGAAGTAGTATCTTAGGATTTGGGATTGGGTTAGCTTTTAAATTTGATATTATGACCTCCCTCTACATCGGAACAGCAACCGCACTAAGTAGTACCATCGTAATCCTCAAACTCTTGGGAGATAAAGATGATATCGATACCACATACGGAAGACTTTCGATCTGA", "aa": "MIGSSILGFGIGLAFKFDIMTSLYIGTATALSSTIVILKLLGDKDDIDTTYGRLSI", "aa_hexdigest": "37a0d5262544c809cfd159a1ddf88ee3", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5928.9048999999995, "isoelectric_point": 4.687136650085448 }, "id": "NKOCPLAKPK_344", "locus": "GCA003260355_00307" }, { "type": "cds", "sequence": "contig_1", "start": 155199, "stop": 155408, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00999.27" ], "nt": "ATGGATCAGTTTTTTCTCTCATTCGCTGTAATTCTAGGAATCGTCCTCCTCTCGCTAGGAGCAATGAAAATCCTTAAGCAACCAATGATTATCGGATATATTCTCGCGGGGATCGTAATCTCTTTTCTTTTCCCTGGAGTTTTCGCAGAATCAGAAACCTTTACTCAGCTTTCGCAGGTAGGAATCACATTTCTGCTCTTCATCATCTGA", "aa": "MDQFFLSFAVILGIVLLSLGAMKILKQPMIIGYILAGIVISFLFPGVFAESETFTQLSQVGITFLLFII", "aa_hexdigest": "10f1db376757407f9e6b5fa3878fdd26", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00999.27", "name": "Na_H_Exchanger", "length": 54, "aa_cov": 0.782608695652174, "hmm_cov": 0.9814814814814815, "evalue": 1.4270502648446115e-07, "score": 27.104310989379883, "start": 15, "stop": 68 } ], "seq_stats": { "molecular_weight": 7586.187699999997, "isoelectric_point": 4.677076148986816 }, "id": "NKOCPLAKPK_345", "locus": "GCA003260355_00308" }, { "type": "cds", "sequence": "contig_1", "start": 155611, "stop": 156216, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00254.34" ], "nt": "ATGACTAAGAAATTAGAAAAGAAAGCCTTTCCCCTTTGGGCTAAGGTAGTGATTGGGGTCTGTGGGCTAGGAATCGTTTCAGTAGGAGGGCTATTTGCTCTCAATTATATGAAGGAGGGAAGGAGTTCAACTCCTGAGACGTCAATTACTGGGCAACAAGAACAACAACCTTCAGATTTATCTTCTAATGAACCTCTAAACATGAATGCAGAAGCGGCGATTAAAGACTATTTAACACAAGCAAATCAGGATATTAAAGCTGATCAGGTGGTGGCAAGCGGTGCTACTATCACGGTGGACTATATTGGTAGGCTCAATGATAAGGAAGTTTTTGATACGAGCGTAAAAAGTATAGCTGAAGCTGCTGGAACGTATTCTCCTCAGAGAAATTATGATGAAGGACTAAGTTTTACGGTAGGAGCTGGTCAAATGATCAAGGGATTTGATGAAGCTGTCGTAGGTATGAAGGTTGGAGAGACAAAAACTGTGCATATCCCTGCTGAGAAAGCATATGGAAAGAGAGATGAGAACTTGATTCTTCGTGTGCCTTTGGAGCAGGCGGGAGATACTTCAGGAGCTCAAGTAGGAATGAAAGTGCTTGTTTGA", "aa": "MTKKLEKKAFPLWAKVVIGVCGLGIVSVGGLFALNYMKEGRSSTPETSITGQQEQQPSDLSSNEPLNMNAEAAIKDYLTQANQDIKADQVVASGATITVDYIGRLNDKEVFDTSVKSIAEAAGTYSPQRNYDEGLSFTVGAGQMIKGFDEAVVGMKVGETKTVHIPAEKAYGKRDENLILRVPLEQAGDTSGAQVGMKVLV", "aa_hexdigest": "1c522863f3d4da5802219969dd29b343", "start_type": "ATG", "rbs_motif": "TTTA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00254.34", "name": "FKBP_C", "length": 86, "aa_cov": 0.42786069651741293, "hmm_cov": 0.8372093023255814, "evalue": 1.6393659133656393e-20, "score": 69.6854248046875, "start": 91, "stop": 176 } ], "seq_stats": { "molecular_weight": 21504.173399999978, "isoelectric_point": 5.152989006042478 }, "id": "NKOCPLAKPK_346", "locus": "GCA003260355_00309" }, { "type": "cds", "sequence": "contig_1", "start": 156366, "stop": 156641, "strand": "+", "frame": 3, "gene": null, "product": "DUF5673 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4KRA6", "UniRef:UniRef90_A0A660MTX5" ], "nt": "ATGGCCACTTTGAAAGTCTATAATGGAGAGCTTTTTGAAAGAGGAAAGCGATGGTATGTCGGTTTTGCTGGCGTTATTTTGACGATTCTTGGGCTTTCTCTTTTTTACAAGACAGGACAAGGAGTGCAGAACATCGTAGGAAGCGTAATTATGCTGATGATTGTCGGAGGCTACCTTTTCTTTCAGTCGAAAACGTGGTCAACGATTCAGCTTACGCTTAGTTCAGAAGGAGTTTTTGCAGGAGAGAGGTTGATCCCTTTTGCTGTGCTTAAATGA", "aa": "MATLKVYNGELFERGKRWYVGFAGVILTILGLSLFYKTGQGVQNIVGSVIMLMIVGGYLFFQSKTWSTIQLTLSSEGVFAGERLIPFAVLK", "aa_hexdigest": "44e6106fc32aa3ed8bc9437bdaeb3e69", "start_type": "ATG", "rbs_motif": "TTCTT", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 156366, "stop": 156842, "observations": { "mutation": [ 156639 ], "stop": [ 156639 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A660MTX5", "query_cov": 1.0, "subject_cov": 0.5759493670886076, "identity": 1.0, "score": 184.0, "evalue": 4.39e-58, "gene_start": 1, "gene_end": 91, "reference_start": 1, "reference_end": 91, "reference_sequence": "MATLKVYNGELFERGKRWYVGFAGVILTILGLSLFYKTGQGVQNIVGSVIMLMIVGGYLFFQSKTWSTIQLTLSSEGVFAGERLIPFAVLKGFVVEMEKKTGKLRNIVFVFDKSVEIFTLNDTPEHQKLFFAELAKVVPFLESYEQGFVEKLIRKIKL" }, "paralog": false, "identity": 0.9936708860759493, "score": 307.0, "evalue": 4.73e-109, "description": "Internal stop codon at 156639. Nonsense mutation around 156639.", "psc": { "uniref90_id": "UniRef90_A0A660MTX5", "product": "DUF5673 domain-containing protein", "uniref50_id": "UniRef50_A0A2W4KRA6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A660MTX5", "UniRef:UniRef50_A0A2W4KRA6" ] }, "pscc": { "uniref50_id": "UniRef50_A0A2W4KRA6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4KRA6" ], "product": "DUF5673 domain-containing protein" } }, "id": "NKOCPLAKPK_347", "locus": "GCA003260355_00310" }, { "type": "cds", "sequence": "contig_1", "start": 156654, "stop": 156842, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAGAAAAAGACTGGGAAACTCAGAAATATCGTCTTCGTCTTTGATAAATCGGTTGAAATCTTTACACTGAATGATACTCCTGAGCATCAAAAACTTTTTTTTGCAGAGCTAGCAAAAGTAGTGCCTTTCTTGGAATCTTATGAGCAGGGGTTCGTGGAGAAATTAATTAGAAAGATAAAGCTTTAA", "aa": "MEKKTGKLRNIVFVFDKSVEIFTLNDTPEHQKLFFAELAKVVPFLESYEQGFVEKLIRKIKL", "aa_hexdigest": "c5ba04c20bed761c7fab24e8de8ed66b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7331.573599999997, "isoelectric_point": 9.049443244934082 }, "id": "NKOCPLAKPK_348", "locus": "GCA003260355_00311" }, { "type": "cds", "sequence": "contig_1", "start": 156958, "stop": 157170, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTGTAGAAGCTGTTTGTCGAAAGTTTTTTTACCCTAAATGGGAGTTTTTTTGGTTTTTTTGTGTTGGAAATAGTGAAAAACTGCTACTTTTCTCATTTCCTAGGGCTACAATCGTTCCTTGTAGGCGTGTAAAGGCGAATTTGAGGGGGCAGAGGCATGAAGGTTACTCTTGTGAGGAGGAATCCTCAACATATATGTCTTCATCTTGA", "aa": "MFVEAVCRKFFYPKWEFFWFFCVGNSEKLLLFSFPRATIVPCRRVKANLRGQRHEGYSCEEESSTYMSSS", "aa_hexdigest": "fd0030b222c6a2c17e068089e03e5733", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8362.560000000001, "isoelectric_point": 8.816067695617676 }, "id": "NKOCPLAKPK_349", "locus": "GCA003260355_00312" }, { "type": "cds", "sequence": "contig_1", "start": 157227, "stop": 157463, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGATACTTATGCTTATTTTGCTTATGGTGCAGCTCGCTTTCTCCCAGAAGATTTTGCTTTCTCATCCTCATACTCCAGATGATCAAATAGGGAGAGCGCCCCAGACATACGAATCCACGGCTCAGTCAATTCCGCATGATCAAGGAAATGTCAAAAAAGACGCCTCAGAGAAGCAGCGTCAGGAACAGATTAATGTTCACAAAGAGTCACCTCTACAACCCCTTCCTTCTTGA", "aa": "MKILMLILLMVQLAFSQKILLSHPHTPDDQIGRAPQTYESTAQSIPHDQGNVKKDASEKQRQEQINVHKESPLQPLPS", "aa_hexdigest": "2ad4f4064a37bb67f71698e9390be58c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8824.045900000001, "isoelectric_point": 6.900844764709473 }, "id": "NKOCPLAKPK_350", "locus": "GCA003260355_00313" }, { "type": "cds", "sequence": "contig_1", "start": 157512, "stop": 157835, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACGAAGAATTTCTCCAAAGTTAGGAACCTAGAAGACTTTATGGAGAACTGTAGCTTCGAGGAAGGATATAGCGTGTATGATAATGCTGGTCGTGGCGGGACACAAGACCAGCTCAGTATCCTCAAGGATGCGATAACCGACGCTCGAGGATCAGAATCTGATGAACGCAGGTTTTGTAAGTCCGATCTGACAGATATAAATCTTTTGCCAGATAAAGCCTATCAGGATTTTATTGCCTTTCTAGAGAAACATCGCCTTAGACAGAAGTCTTTGTTGGCGATCGTATTTCCTGCTCCGCTCGATGAGTTTTATTCCTTCTGA", "aa": "MTKNFSKVRNLEDFMENCSFEEGYSVYDNAGRGGTQDQLSILKDAITDARGSESDERRFCKSDLTDINLLPDKAYQDFIAFLEKHRLRQKSLLAIVFPAPLDEFYSF", "aa_hexdigest": "23c4b08137612de5ed642b14090a32cc", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12343.671899999983, "isoelectric_point": 4.815706443786621 }, "id": "NKOCPLAKPK_351", "locus": "GCA003260355_00314" }, { "type": "cds", "sequence": "contig_1", "start": 158229, "stop": 159074, "strand": "-", "frame": 2, "gene": null, "product": "Peptidase C51 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EFW0", "UniRef:UniRef90_A0A328EFW0" ], "nt": "ATGCTTATGATGGTAGTATCCGCAGGGATATTTACCTCGTGTGAACAGAATACCCCTAATGAGGACAATCGTCCTCTTGGGGAAGGCAATCTCGTTTCTACCCTGAGGAACGCAAGTTCCAGCTCTCTGGGGACGTTCGAGGTTTATCCGTATTCAGGTTATGTGTATGATACTGGGTTTACGTTTACATTTTATTCCTCGTTTCCGCATTTTGCAAATCCGAGGGTGAGAGTCTTTGTAAAATTCAACTCTCCTCTAGGTTTTACAGGGTATGAAGAGATGCATAAAATAGACGTATCCAGCTCTGGAGTAACATTCCAGTTTATGAAAGATCGTTTCCGAGAAGTTGGGACTTATCGCTGTGAGATCGCATTCTCAATTGATGGAGATGATCCTAAATATCCTTTGGGAATTGCTCAATCCATCTCGGTAAACTCTAATGCTGGACCTGCTATTGACGACTATAATAGTAGCAATAACAGTGGATACTCCGATAGGTATTGCACAGCTTGGGTAGCGTGGAAGGTTAACCAAATGTGGGGAACTTGGAACGCTTTCCCGAGTGGCTTAGGAAATGCTGAAGACTGGAAAGATGCCTTAGATGGTGCTGGCTATGACATCGAACAAGGTCGTGCTATACCAGGAGATATTATCTGGTTCTCTCCAGGGACTCATCATATAGAACCTCCTGGACACGTTGGTTTCGTTCATAGCGTTACAAACTCTACCATCTACTACACGGATTATAATGGAGGGAAACCTCAGAACAAAACCTTTGGTAATCGCAAGATTTCAACGAGTTCTGCCTCTGATGTCTTCTTCATCCATGTGCAAAAACAAAAGTAA", "aa": "MLMMVVSAGIFTSCEQNTPNEDNRPLGEGNLVSTLRNASSSSLGTFEVYPYSGYVYDTGFTFTFYSSFPHFANPRVRVFVKFNSPLGFTGYEEMHKIDVSSSGVTFQFMKDRFREVGTYRCEIAFSIDGDDPKYPLGIAQSISVNSNAGPAIDDYNSSNNSGYSDRYCTAWVAWKVNQMWGTWNAFPSGLGNAEDWKDALDGAGYDIEQGRAIPGDIIWFSPGTHHIEPPGHVGFVHSVTNSTIYYTDYNGGKPQNKTFGNRKISTSSASDVFFIHVQKQK", "aa_hexdigest": "3d73aaa0e624c9c81c754777586dcf67", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A328EFW0", "query_cov": 1.0, "subject_cov": 0.972318339100346, "identity": 1.0, "score": 583.0, "evalue": 9.23e-211, "valid": true, "product": "Peptidase C51 domain-containing protein", "uniref50_id": "UniRef50_A0A328EFW0" }, "pscc": { "uniref50_id": "UniRef50_A0A328EFW0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EFW0" ], "product": "Peptidase C51 domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_352", "locus": "GCA003260355_00315" }, { "type": "cds", "sequence": "contig_1", "start": 159485, "stop": 159838, "strand": "-", "frame": 3, "gene": null, "product": "Lipoprotein", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBFAB94", "UniRef:UniRef100_A0A328EFG2", "UniRef:UniRef50_A0A328EFG2", "UniRef:UniRef90_A0A328EFG2" ], "nt": "ATGAAAAAAAGAATGCTGATCCTCATCATAGGAAGCTTAGTATTAACAGGATGTAATTCACGAAAAACGATTACCGACACCCCTGAATACCAAAAAACTCAGCAAATAGCCGCCTCTGGAATGAACTATGTCTCAGAGAAAACTGCAGATTTCATCAAAGAAAACGAGCAAGTCCAAGGAGCCATCACCGCCACCCAAGAGGGAGTAAAACAGCTTAAGGGTCAAGCAGAGGACTTAGCAAACCAGGCTAAAGATGAGCTGAATAAACAATACAATCAGCTCAAAGAAGACGCAAAAACTTCTGTCAAGGATTCCGTAAACCAGAAAATAGACAAAGCTTTTGAAAAACTCTAA", "aa": "MKKRMLILIIGSLVLTGCNSRKTITDTPEYQKTQQIAASGMNYVSEKTADFIKENEQVQGAITATQEGVKQLKGQAEDLANQAKDELNKQYNQLKEDAKTSVKDSVNQKIDKAFEKL", "aa_hexdigest": "db0ddf87ca47d6a679a8b5165983dcd6", "start_type": "ATG", "rbs_motif": "TTCTT", "ups": { "uniparc_id": "UPI000DBFAB94", "uniref100_id": "UniRef100_A0A328EFG2", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBFAB94", "UniRef:UniRef100_A0A328EFG2" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EFG2", "uniref90_id": "UniRef90_A0A328EFG2" }, "psc": { "uniref90_id": "UniRef90_A0A328EFG2", "product": "Lipoprotein", "uniref50_id": "UniRef50_A0A328EFG2" }, "pscc": { "uniref50_id": "UniRef50_A0A328EFG2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EFG2" ], "product": "Lipoprotein" }, "genes": [], "id": "NKOCPLAKPK_353", "locus": "GCA003260355_00316" }, { "type": "cds", "sequence": "contig_1", "start": 160040, "stop": 161485, "strand": "-", "frame": 3, "gene": null, "product": "PIN domain-containing protein", "db_xrefs": [ "KEGG:K07175", "SO:0001217", "UniRef:UniRef50_A0A328EHR5", "UniRef:UniRef90_A0A328EHR5" ], "nt": "ATGGCAAAAAGAAAAGAGGATTCCCCTCGTAACAAAAAGAACAAAAAAAAAGGAAAAACAAAAGGCGAGATTTCATCTTTTCCAAGCGAAACCGAGAGCGAGAAGGAAAAGAAAACCTACATTCTGGATACGAACGTCGTCCTGAATGATCCGTATTGCCTGAAACATTTTGGGAAACACAATATTTGTATCCCTATAATGGTGCTCGAAGAGCTCGATAAATTCAAAAAAGGAAATGAACCCAACAATGTCAATACTAGAATATTTCATAGAATATTCAACGGATTTGGAGATGGGCTCTTTAACGGAGGGATTTCCTTGGGAGAAAATCGAGGGAAACTAACAGTTGCATTGGGGGTCGACTATCCTCGAGAGATGGAAAAATCATTCTACAAAGATACTCCAGACCATCGGATACTGGCAATTGCACTGGACAAAAAAAGGAAAGGAGAAAACGTCGTTTTGGTAACAAACGACATCAATCTCCAGATCAAAGCACGAGCATTAGGAGTGAAATCTGAATCTTATCGCCACGATAGCGTGAAAGATTTAGAAAGTATCCAAGCATCAGTGATCTCCTACCGCCCCAGAAGCGAAGGCTGGGGGGAGCTAACTGAAGATATGGGGAATACGAATGGATATCGCGTCCCCAAAAGTCTGAGCAAGTATCCAGAAAATTCTCTATTCATCATCCTATCAGGGAAAGGAGAAAATAGAAAAGAATACTTGGTCAGGAAATGTGGAAAATACCTAATGCCAGTAGGATACTACAACGGAAAAGAATCCGTATACGGCATCGAAGCTAAAAATAATGAACAGCGATTCTGCTTTGATGCTCTGCTTGATGATAACATCCCGCTCGTGGCAATAACCGGCAACGCTGGAACAGGAAAAACCATCATCAGTCTTGCAGCAGCACTTCATCAAAAAGATCCATACGAGGAGATCCTAGTTGCTCGTCCCGCTATAGAGATGAGCAATAAGACACTAGGATACTTGCCAGGCAAGCTAGACGGGAAAATCGATCCCTACATGAAACCTATCTTCGATAACCTCGCAATAATAAAAGAGGCAAACGAAAAGAAAAAAGGGAAAAAGAAGGGAACGAGCCAGTCCGTCGAAGATTGGGCAAAGGAGCAGAAGATCAGCACCCTCGTCTTAAACTTCGTCCGTGGTCGATCTCTAGCAAACCGTTTCATCATCATCGATGAAGCACAGAATCTGACGCCTCACGAGATTAAAACGATCATAACCCGAGCTGCAAAGGGGACAAAAATCGTGTTAGTTGGAGATATTAGTCAGGTAGATTCGCCATATCAAAACGAACGATCGAATGGGCTTTCCTATTTGATCGATCGATTCCGAGGGCAACCTGAATTTGCGCATATCCATCTCAATAATGGAGAGCGGAGCCCTCTCGCCGCCTTGGCGGGACAGCTCTTGTAA", "aa": "MAKRKEDSPRNKKNKKKGKTKGEISSFPSETESEKEKKTYILDTNVVLNDPYCLKHFGKHNICIPIMVLEELDKFKKGNEPNNVNTRIFHRIFNGFGDGLFNGGISLGENRGKLTVALGVDYPREMEKSFYKDTPDHRILAIALDKKRKGENVVLVTNDINLQIKARALGVKSESYRHDSVKDLESIQASVISYRPRSEGWGELTEDMGNTNGYRVPKSLSKYPENSLFIILSGKGENRKEYLVRKCGKYLMPVGYYNGKESVYGIEAKNNEQRFCFDALLDDNIPLVAITGNAGTGKTIISLAAALHQKDPYEEILVARPAIEMSNKTLGYLPGKLDGKIDPYMKPIFDNLAIIKEANEKKKGKKKGTSQSVEDWAKEQKISTLVLNFVRGRSLANRFIIIDEAQNLTPHEIKTIITRAAKGTKIVLVGDISQVDSPYQNERSNGLSYLIDRFRGQPEFAHIHLNNGERSPLAALAGQLL", "aa_hexdigest": "9f31ad525e6c1d4a1487af0f43f7380e", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A328EHR5", "query_cov": 0.9501039501039501, "subject_cov": 0.8856589147286822, "identity": 1.0, "score": 914.0, "evalue": 0.0, "valid": true, "product": "PIN domain-containing protein", "uniref50_id": "UniRef50_A0A328EHR5", "kegg_orthology_id": "K07175" }, "pscc": { "uniref50_id": "UniRef50_A0A328EHR5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EHR5" ], "product": "PIN domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_354", "locus": "GCA003260355_00317" }, { "type": "cds", "sequence": "contig_1", "start": 161797, "stop": 162291, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01425.27" ], "nt": "ATGCAACTTACGATCGCGTCCTATCTTCAAGGACTGAAGAATAAGGATTTTAGCCCCAGAGAAGTCCTCCTTGATTATCAAAAAAGAGCTAAAGCTCTTAATACTGAACTTAATGCCTGTGTGCGTTTTAATGATGAGTATGTAAATGCTCATTTTGAGGAATTTTCTACTAGAGCATTAGCTTCGCTTCCGATTATGGTTAAGGATAATATCCTCGTTAAAGGGCAGTCGGTCACTTGTTGTTCTAAAATGCTTGAAGGTTATACTGCTCCTTATTCAGCGAGCTGTATGCAGAATCTAGAAAAGGCTGGTTGCTTGATGATTGGTCAGACAAATATGGATGAATTTGCCATGGGAGGCTCTACGGAAACTTCATTTTATGGGAAAACGCTTAATCCTGTTTGTGCAAGTCGTATTCCTGGTGGAAGTAGTGGCGGCTCCGCAGCTGCGGTTGCTGCGAATATGGCGATTGCTGCTCTGGGGACCGATACGTGA", "aa": "MQLTIASYLQGLKNKDFSPREVLLDYQKRAKALNTELNACVRFNDEYVNAHFEEFSTRALASLPIMVKDNILVKGQSVTCCSKMLEGYTAPYSASCMQNLEKAGCLMIGQTNMDEFAMGGSTETSFYGKTLNPVCASRIPGGSSGGSAAAVAANMAIAALGTDT", "aa_hexdigest": "05ed84a0950fe2428b90c0f3a587533b", "start_type": "ATG", "rbs_motif": "TTATT", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01425.27", "name": "Amidase", "length": 151, "aa_cov": 0.8780487804878049, "hmm_cov": 1.0, "evalue": 3.6882803772605905e-49, "score": 164.3806915283203, "start": 21, "stop": 164 } ], "seq_stats": { "molecular_weight": 17531.876399999983, "isoelectric_point": 5.818800926208497 }, "id": "NKOCPLAKPK_355", "locus": "GCA003260355_00318" }, { "type": "cds", "sequence": "contig_1", "start": 162316, "stop": 162405, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01425.27" ], "nt": "ATGTGTGGGATTGTCGGATTTAAGCCAAGCTATGGTGCGATTTCTCGCTATGGGGTGGTGGCAATGGCGTCGTCCTTGGATCAGGTTTGA", "aa": "MCGIVGFKPSYGAISRYGVVAMASSLDQV", "aa_hexdigest": "de30177cb6c6027f4b4f10c4dfe0577d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01425.27", "name": "Amidase", "length": 29, "aa_cov": 1.0, "hmm_cov": 1.0, "evalue": 1.4271333321603985e-10, "score": 37.20131301879883, "start": 1, "stop": 29 } ], "seq_stats": { "molecular_weight": 3007.5050999999985, "isoelectric_point": 7.933302116394044 }, "id": "NKOCPLAKPK_356", "locus": "GCA003260355_00319" }, { "type": "cds", "sequence": "contig_1", "start": 162421, "stop": 162750, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01425.27" ], "nt": "GTGGAAGATGTGCAGCTTTTGTTTCCTTATTTAGCTGGTTATGATGATAAAGATGGGACTTCATCTTTGCAGGCTGAGTCTTTAAAAGAAAAAAAATCTGAATTCCCTTCTCCGTATAGGATTTTTGTGCCAGAAGAAGCTCTCTCAGAAGGCGTTCATCCACAGATCAAAGAGCTTTTTTTGCAGAAGTTAGAAATCCTAAAGGCTCAGGGACATCAAGTGGTTATTGATTCTTTGCCGATTATGAAGCATGCACTTGCGGTTTATTATGCCTTGATGCCGGCTGAGGTAAGCACGAATCTTTCTCGTTTTGATGGGATTAGATTTTGA", "aa": "MEDVQLLFPYLAGYDDKDGTSSLQAESLKEKKSEFPSPYRIFVPEEALSEGVHPQIKELFLQKLEILKAQGHQVVIDSLPIMKHALAVYYALMPAEVSTNLSRFDGIRF", "aa_hexdigest": "e59c3e9f255d3036244b0f2debf17cf2", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01425.27", "name": "Amidase", "length": 109, "aa_cov": 0.9724770642201835, "hmm_cov": 1.0, "evalue": 3.88577623146455e-15, "score": 52.24686050415039, "start": 2, "stop": 107 } ], "seq_stats": { "molecular_weight": 12357.066299999988, "isoelectric_point": 5.097286796569824 }, "id": "NKOCPLAKPK_357", "locus": "GCA003260355_00320" }, { "type": "cds", "sequence": "contig_1", "start": 162907, "stop": 163149, "strand": "+", "frame": 1, "gene": null, "product": "Aspartyl-tRNA(Asn) amidotransferase subunit A Glutamyl-tRNA(Gln) amidotransferase subunit A", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A482ZSY6" ], "nt": "GTGCAGAAGCTTTTGAAGGCTGAACTAGCGAAGATCTATCAAGAGTATGATGTCTTATTGACTCCAACGACTCCAGAGCCAGCTTGGAAATTGGGATCAAAAACTGCCGATCCTCTCAAGATGTATCTTTCTGATCTCTATACGGTTCCTGCGAATTTGGCTGGGTTGCCTGCGATTTCTGTTCCACTGGGAATGGTAGATGAAGAGGGAGATCAGCTTCCTGTTGGAATCCAAGCGATGTGA", "aa": "MQKLLKAELAKIYQEYDVLLTPTTPEPAWKLGSKTADPLKMYLSDLYTVPANLAGLPAISVPLGMVDEEGDQLPVGIQAM", "aa_hexdigest": "1b6de575b132992cd7a9d54f09bbd01e", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001400A64F", "query_cov": 0.975, "subject_cov": 0.8571428571428571, "identity": 0.513, "score": 87.4, "evalue": 6.16e-21, "valid": false, "product": "amidase family protein", "uniref50_id": "UniRef50_A0A482ZSY6" }, "pscc": { "uniref50_id": "UniRef50_A0A482ZSY6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A482ZSY6" ], "product": "Aspartyl-tRNA(Asn) amidotransferase subunit A @ Glutamyl-tRNA(Gln) amidotransferase subunit A" }, "genes": [], "id": "NKOCPLAKPK_358", "locus": "GCA003260355_00321" }, { "type": "cds", "sequence": "contig_1", "start": 163465, "stop": 163977, "strand": "+", "frame": 1, "gene": "rplJ", "product": "50S ribosomal protein L10", "db_xrefs": [ "GO:0003735", "GO:0006412", "GO:0015934", "KEGG:K02864", "SO:0001217", "UniParc:UPI000DBFF2B9", "UniRef:UniRef100_A0A328EG39", "UniRef:UniRef50_A0A660MSL7", "UniRef:UniRef90_A0A660MUM1" ], "nt": "ATGACAATCTCAAAAGAGAAAAAAGTAGAGCTAGTAAAACAGTATGTTCAAGACTTACAATCTGCAAAGAATGTAGTGATCCTTCAGCAGTCTGGAATCCCAGTAGTGGGAGCTACTCAGATGAGAAAAGGAGTTCTTGAAGCTGATGGTAAATTTAATGTCATCAGAAAGAAACTGTTTTTACTAGCGCTCAAGGAAGCTGGTTACGAAGATGTAGCTGAAAGCGATCTTGAGGGATCAGTAGTTGTTCTCTATGCAAATGGAGATGAATACGGTCCAATGAAGGTTGTTAATAAGTATGCAAAAGAATTTGCAGCTGATAAGGAGCTTAAAGCTTCTATCAAGTTCCTTGGAGGGTGGTATGACAAGAAGTGGCATAATTCAGAATATGTTACTGAACTTGCAAATATCCCATCAAGAGAAGAGCTGCTTTCAAAATTGGCATACCTATTCAATTACCCTTTACAGTCAGTTGCTTGTGTGATTGACCAAATTGCAAAAAAAGCTGGATAG", "aa": "MTISKEKKVELVKQYVQDLQSAKNVVILQQSGIPVVGATQMRKGVLEADGKFNVIRKKLFLLALKEAGYEDVAESDLEGSVVVLYANGDEYGPMKVVNKYAKEFAADKELKASIKFLGGWYDKKWHNSEYVTELANIPSREELLSKLAYLFNYPLQSVACVIDQIAKKAG", "aa_hexdigest": "b49505e424f714527275aebb73bd9357", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000DBFF2B9", "uniref100_id": "UniRef100_A0A328EG39", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBFF2B9", "UniRef:UniRef100_A0A328EG39" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EG39", "uniref90_id": "UniRef90_A0A660MUM1" }, "psc": { "uniref90_id": "UniRef90_A0A660MUM1", "gene": "rplJ", "product": "50S ribosomal protein L10", "uniref50_id": "UniRef50_A0A660MSL7", "kegg_orthology_id": "K02864", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015934" ] }, "pscc": { "uniref50_id": "UniRef50_A0A660MSL7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MSL7" ], "product": "Large ribosomal subunit protein uL10" }, "genes": [ "rplJ" ], "id": "NKOCPLAKPK_359", "locus": "GCA003260355_00322" }, { "type": "cds", "sequence": "contig_1", "start": 164027, "stop": 164410, "strand": "+", "frame": 2, "gene": "rplL", "product": "50S ribosomal protein L7/L12", "db_xrefs": [ "GO:0003735", "GO:0005840", "GO:0006412", "SO:0001217", "UniParc:UPI000DBFB320", "UniRef:UniRef100_A0A660MW25", "UniRef:UniRef50_A8YTF2", "UniRef:UniRef90_A0A660MW25" ], "nt": "ATGGAATTAACTAAAAATCAACAACAGATTTTGGATCTTGTAAAAGAGATGAATGCTGTTGAACTTAATGGGCTTGTAAAAGCTTTAGAAGAAGAATTTGGAGTAACTGCTGCTGCTATGGTAGTTGCTGGAGGTGCTGCTGCTGGAGCTGGTGCTGCAGACGCTGGTGCATCTGACGCTATGAATGTTGAGCTTACTGATGCTGGACAACAGAAAATCGCTGTAATTAAGGTGGTTAAAGAAGTTCTTGGACTTGACCTTAAGGCTGCTAAAGAACTAGTAGAAAAAGCTCCTGTAATCATCAAGGAAAATGCTAAGTCTGATGAAGCTGAGGCTTTGAAGGCTAAGCTTAGCGAAGCTGGAGCTACTGTAAACTTCAAATAG", "aa": "MELTKNQQQILDLVKEMNAVELNGLVKALEEEFGVTAAAMVVAGGAAAGAGAADAGASDAMNVELTDAGQQKIAVIKVVKEVLGLDLKAAKELVEKAPVIIKENAKSDEAEALKAKLSEAGATVNFK", "aa_hexdigest": "c6a27d3f5fbfecfbbe980a0c037ab0a4", "start_type": "ATG", "rbs_motif": "TTATT", "ups": { "uniparc_id": "UPI000DBFB320", "uniref100_id": "UniRef100_A0A660MW25", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBFB320", "UniRef:UniRef100_A0A660MW25" ] }, "ips": { "uniref100_id": "UniRef100_A0A660MW25", "uniref90_id": "UniRef90_A0A660MW25" }, "psc": { "uniref90_id": "UniRef90_A0A660MW25", "gene": "rplL", "product": "50S ribosomal protein L7/L12", "uniref50_id": "UniRef50_A8YTF2", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_A8YTF2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A8YTF2" ], "product": "Large ribosomal subunit protein bL12" }, "genes": [ "rplL" ], "id": "NKOCPLAKPK_360", "locus": "GCA003260355_00323" }, { "type": "cds", "sequence": "contig_1", "start": 164521, "stop": 165186, "strand": "+", "frame": 1, "gene": "rpsB", "product": "30S ribosomal protein S2", "db_xrefs": [ "GO:0003735", "GO:0006412", "GO:0015935", "SO:0001217", "UniParc:UPI000DBFB7BB", "UniRef:UniRef100_A0A328EJB4", "UniRef:UniRef50_A0A288DIT8", "UniRef:UniRef90_A0A660MWP1" ], "nt": "ATGTCTGTATCTGTTCAAGATATTGTTGCTGCACAGGCTCACATTGGGACGCTTAAAAATGAAGCTCATCCAAAGACAAGTAAATACTGGTCTGAGGTAACCAACGGAGTGGTAGTGATCAATCCAGAAGCGATTGCTGAACAACTTGAGGCTGCAAAAGCACAGGTTGCTGCTGCGAAGGCTGCTGGAAAGGAAATCTTGGTTGTATGTGAAAAGAAAATGTATGCTGAAGAACTTGAAGCATTAGCTGCAAAGGCTGGCGTTGCATATCTCAACTACAAGGTTTCTGGAGGGTTCTTAACGAACTTTGATACCTTCAAGAAAAGAATTGATTCTATCAATGAAATGGTGAGCTTTATGGAAACTGATGCTTTCGCATCTTTGACCAAGAAGGAGCAGCTCGTGTACAAAAGAAACTTTGAAAGAGCAAATAAGGTCTATAAGGGAGTAACAAAACTCAAGAAAAGACCAGAACTCGTAATTATTGTGGATGGAAATATGCTTTCTACCCTTATCGATGAAGTAGAGAATCTCAAGGGAAAACTTGAAGCGATCGTAATCGCAGGGACCAACTTCTCAAGATATTGGCCAGAAAACGAGATTATTACCACCAATATCAATAGTTATCAGAGTTTGGATTTTGTTTTGAAGTCTATTCTCCTCTAA", "aa": "MSVSVQDIVAAQAHIGTLKNEAHPKTSKYWSEVTNGVVVINPEAIAEQLEAAKAQVAAAKAAGKEILVVCEKKMYAEELEALAAKAGVAYLNYKVSGGFLTNFDTFKKRIDSINEMVSFMETDAFASLTKKEQLVYKRNFERANKVYKGVTKLKKRPELVIIVDGNMLSTLIDEVENLKGKLEAIVIAGTNFSRYWPENEIITTNINSYQSLDFVLKSILL", "aa_hexdigest": "cb0741843174cea9221092de457079fe", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000DBFB7BB", "uniref100_id": "UniRef100_A0A328EJB4", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBFB7BB", "UniRef:UniRef100_A0A328EJB4" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EJB4", "uniref90_id": "UniRef90_A0A660MWP1" }, "psc": { "uniref90_id": "UniRef90_A0A660MWP1", "gene": "rpsB", "product": "30S ribosomal protein S2", "uniref50_id": "UniRef50_A0A288DIT8", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015935" ] }, "pscc": { "uniref50_id": "UniRef50_A0A288DIT8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A288DIT8" ], "product": "Small ribosomal subunit protein uS2" }, "genes": [ "rpsB" ], "id": "NKOCPLAKPK_361", "locus": "GCA003260355_00324" }, { "type": "cds", "sequence": "contig_1", "start": 165322, "stop": 165771, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBF79F5", "UniRef:UniRef100_A0A660MW37", "UniRef:UniRef50_A0A7W4EBW4", "UniRef:UniRef90_A0A660MW37" ], "nt": "ATGCAAGAACAACTTTACTTGCCGAGTTCGTCTGAAAGAAAGAGAGTAATTACTGCCTATCTCTTGATAGGGCTCTTCTTTTTTTCAGGCAAGGAGGAATTAACGGTGTATGAATACTTTCATTATAAGCAGGCGATTGGATGGTATATGTCTACGATGGCATTGCTACTTTTCCAACTCATGCTCGTATGGATCCCGATATTGAAATGGTTGCCTTTCCTCTTTGTATTGGCAAATCTTGTCATTGTTGTGATCTTTGTCTTTGGGGCATGGAAGGGGCAGTATGCTCGCGATGTTCTCCAAGAGCATAGATGGAAGATGTTTTATGCTGATCTCGGTGGACGATTGTTGGGAATCTTTGATATTAAGAAGAGGGTGTATGGAGATATTGCAACTGATACTACTGCAGAGAATCTTCCGACGCAGGCTCCCTCACAACCTCTTAGCTAG", "aa": "MQEQLYLPSSSERKRVITAYLLIGLFFFSGKEELTVYEYFHYKQAIGWYMSTMALLLFQLMLVWIPILKWLPFLFVLANLVIVVIFVFGAWKGQYARDVLQEHRWKMFYADLGGRLLGIFDIKKRVYGDIATDTTAENLPTQAPSQPLS", "aa_hexdigest": "16a8db59cede16cb59af2d7a9f387440", "start_type": "ATG", "rbs_motif": "TTATT", "ups": { "uniparc_id": "UPI000DBF79F5", "uniref100_id": "UniRef100_A0A660MW37", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBF79F5", "UniRef:UniRef100_A0A660MW37" ] }, "ips": { "uniref100_id": "UniRef100_A0A660MW37", "uniref90_id": "UniRef90_A0A660MW37" }, "psc": { "uniref90_id": "UniRef90_A0A660MW37", "uniref50_id": "UniRef50_A0A7W4EBW4" }, "pscc": { "uniref50_id": "UniRef50_A0A7W4EBW4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7W4EBW4" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17398.313899999994, "isoelectric_point": 8.802787208557131 }, "id": "NKOCPLAKPK_362", "locus": "GCA003260355_00325" }, { "type": "cds", "sequence": "contig_1", "start": 165891, "stop": 166685, "strand": "+", "frame": 3, "gene": "tsf", "product": "translation elongation factor Ts", "db_xrefs": [ "GO:0003746", "GO:0006414", "SO:0001217", "UniParc:UPI000DBF7BDC", "UniRef:UniRef100_A0A328EJW5", "UniRef:UniRef50_A0A288DF70", "UniRef:UniRef90_A0A2W4KF40" ], "nt": "ATGAATATCGATATTAATTTGGTAAAGCAGCTCAGAGAAGCTACGTTTGCTCCACTTGGAGACTGTAAAAATGCCCTTGTAGAGGCTGGTGGAGACCTTGATAAAGCTCAGGAAATCTTGAAGCAGAAAGGAATCGCAAAAGCAGGCAAGAAAGCTGATAGAGAAACCAACGAAGGACTCATCAAGACGCATCAAGCAAACGGAAGAACTATCGTGCTTAAGCTTCTTTGTGAGACTGACTTTGTGGCGAAAAATGAGACGTTTCTTCAGCTTTTTGATAAGATCTTTGCTGAACTCGAAGGTGTTGCTGGAGAGGTGAGATCGCTCGAGGATCTCGACGCTGCAAAAGTAGAGACGATCAATACGATGCTTGCTGAAGCGGTAGGTTCTATCGGAGAGAATTTGAAGATCGGAGAGCTTTTCATTACTGCTGGAAATGCTTATGCGTATGCGCATCCAGGAAATAAGGTCGTTTCTTTGGTGATGTATAGCGGAGATGAGGCAACTGCGAAAGAATTAGCTTTGCAGGTGGCTGCGTTGAATCCTGACTATCTTAATTTTGATGAGGTGCCTGCCGATGAAAAGGCTAAACTTGAGGCGCAGTTTACTGAAGAGCTTAAAGCAGCAGGGAAACCAGAAGCCATGATCGCAAATATCGTGAAAGGAAAGATTGATAAAGCGTTTGCTGATGTGGTACTTCTTGAACAGGAATATATCAGAGATGGTGCTAAAAAGGTGAAGGAAATCTTGCCTGCTGGATTTGAAGTAACGAAATTCTATAGATTTACGGTGTAG", "aa": "MNIDINLVKQLREATFAPLGDCKNALVEAGGDLDKAQEILKQKGIAKAGKKADRETNEGLIKTHQANGRTIVLKLLCETDFVAKNETFLQLFDKIFAELEGVAGEVRSLEDLDAAKVETINTMLAEAVGSIGENLKIGELFITAGNAYAYAHPGNKVVSLVMYSGDEATAKELALQVAALNPDYLNFDEVPADEKAKLEAQFTEELKAAGKPEAMIANIVKGKIDKAFADVVLLEQEYIRDGAKKVKEILPAGFEVTKFYRFTV", "aa_hexdigest": "008a3adea4b1c0e8164fc52b9528c9ff", "start_type": "ATG", "rbs_motif": "TTCTT", "ups": { "uniparc_id": "UPI000DBF7BDC", "uniref100_id": "UniRef100_A0A328EJW5", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBF7BDC", "UniRef:UniRef100_A0A328EJW5" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EJW5", "uniref90_id": "UniRef90_A0A2W4KF40" }, "psc": { "uniref90_id": "UniRef90_A0A2W4KF40", "gene": "tsf", "product": "translation elongation factor Ts", "uniref50_id": "UniRef50_A0A288DF70", "go_ids": [ "GO:0003746", "GO:0006414" ] }, "pscc": { "uniref50_id": "UniRef50_A0A288DF70", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A288DF70" ], "product": "Elongation factor Ts" }, "genes": [ "tsf" ], "id": "NKOCPLAKPK_363", "locus": "GCA003260355_00326" }, { "type": "cds", "sequence": "contig_1", "start": 166862, "stop": 167962, "strand": "+", "frame": 2, "gene": null, "product": "Peptidase M23 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EG25", "UniRef:UniRef90_A0A328EG25" ], "nt": "ATGAAAACATTCAGAAATTCAATCTTCGGATTGTTTGTGATGATGTTCGTGGTTGTAGTTCTCTTGAGCTCCTGCGAGAAAGATGTGGATCAACCAGTGGTTGTTCCTAGTGCTGAAACCCTTCAGGATGAAGTTCTTGACTCCACTCAGGAGTTAAGATCATCTAGTGGTAGTATGTATTGCGGGATTGTGATGCGAGAAGGGAAAGGGTTCTCGGAAGATACCCATGGTCTTCCCCAGGAAACTCATTATCGTTTCTACGTGGCTAATCCCTCCTCGGATATTACGCGGGTTGATGTCGTCCTTTCTATCCCTTCGGGGGGAACAGAGGTACGTCCTATGGTCAAGAACGGTAAAGGCAACTTCTTCCATGAACAGACGCTTCGTCAGCATGGGAGGTATGATGTGAAGTATCGAATCTTTAGGCGGAGGTCTTTTTCCTATCAGGTCGTTGATCCCAAACCTAATTATGTCGATAATACTAGGGTTTGGTGTGCATCAAATGCTCTAGGGATGTATTGGGTGTTTGCAGATGGATCTTCCCGATCTAATAATTCAGTCTATAAACAGGTTACTGTCGAGAAGAAACCAGGTGATAAACCAGGCAATGAAAAAGAATGGAAACGATTTAAGTGGCTGAATGGTGAGGCTGATGGACGAGGCTTCGGAGGAAGCGGATTTGGAGAGGGGCTACATACCAAAGGAACTTCTGAGGAATATGCTTTGGATTACAACTTGTATCCTTATGATTGGAAGCCTGGTTCTTCGATGGAGATCTATACTGATATCGATAAGGGCACGGCACTTTACTCTCCCCTTGATGGGAAGGTTATCAAGCTAGAGAATAATCCTAAAGCGAAATTTGGGAAATATGTTGTGGTTGAGCAGAAACTTGGTGAAAAAACCTTTCAAGTCTATTTTGGGCACCTAGAAACTATCTCTGTGCAGCGTGGACAGTATGTTATTGGTGGACAAACTGTCGTTGGGACTCTTGGAAATACTGGAGCTTCGGGTGCCTACCACCTGCATATTAGTGCATGGGATGTGACGCGGTCAAAAACTTCAATACAACATGGTATGTTCTTGAAACCTGGAGAGTGA", "aa": "MKTFRNSIFGLFVMMFVVVVLLSSCEKDVDQPVVVPSAETLQDEVLDSTQELRSSSGSMYCGIVMREGKGFSEDTHGLPQETHYRFYVANPSSDITRVDVVLSIPSGGTEVRPMVKNGKGNFFHEQTLRQHGRYDVKYRIFRRRSFSYQVVDPKPNYVDNTRVWCASNALGMYWVFADGSSRSNNSVYKQVTVEKKPGDKPGNEKEWKRFKWLNGEADGRGFGGSGFGEGLHTKGTSEEYALDYNLYPYDWKPGSSMEIYTDIDKGTALYSPLDGKVIKLENNPKAKFGKYVVVEQKLGEKTFQVYFGHLETISVQRGQYVIGGQTVVGTLGNTGASGAYHLHISAWDVTRSKTSIQHGMFLKPGE", "aa_hexdigest": "576f82b4ca32620a0abd15ba52853098", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A328EG25", "query_cov": 0.9617486338797814, "subject_cov": 0.9539295392953929, "identity": 1.0, "score": 717.0, "evalue": 3.61e-261, "valid": true, "product": "Peptidase M23 domain-containing protein", "uniref50_id": "UniRef50_A0A328EG25" }, "pscc": { "uniref50_id": "UniRef50_A0A328EG25", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EG25" ], "product": "Peptidase M23 domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_364", "locus": "GCA003260355_00327" }, { "type": "cds", "sequence": "contig_1", "start": 168053, "stop": 168403, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EFX0", "UniRef:UniRef90_A0A328EJD1" ], "nt": "ATGTTAATGCTTGGGATGTTTGCGATGTATAGCTTTCTTTTTCCTGCGCTCCATCAAGAATCTGAGATTCCCCACCAAAGGGAGACTGTTGATGGGCAAAAACTTACCCGGGAGGGCTACCCTGCCATTCCTGATAACACCGAAGTCCTTTGGTGGGACTGGAAGATTAACTATCCTCCAATGAAGAAAACTTTTTTAGTGAAAGGGGAACTCACGAGGGACTCGTTCTTATGTACTCGACCCCTTGAATGTGATGAAGAGAGAGGAACTCTTATGGAAACTTGGGAGATTGTCTCTAAAGATAACAAATCTACTCCTGAAAAGAAAATTTATTTGGGGGTGACTTCCTGA", "aa": "MLMLGMFAMYSFLFPALHQESEIPHQRETVDGQKLTREGYPAIPDNTEVLWWDWKINYPPMKKTFLVKGELTRDSFLCTRPLECDEERGTLMETWEIVSKDNKSTPEKKIYLGVTS", "aa_hexdigest": "b9acfa599168d1b1fb0c075e51dd94a3", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 168047, "stop": 168595, "observations": { "mutation": [ 168050, 168401, 168458 ], "stop": [ 168050, 168401, 168458 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EJD1", "query_cov": 1.0, "subject_cov": 0.6373626373626373, "identity": 1.0, "score": 245.0, "evalue": 1.63e-81, "gene_start": 1, "gene_end": 116, "reference_start": 3, "reference_end": 118, "reference_sequence": "MGMLMLGMFAMYSFLFPALHQESEIPHQRETVDGQKLTREGYPAIPDNTEVLWWDWKINYPPMKKTFLVKGELTRDSFLCTRPLECDEERGTLMETWEIVSKDNKSTPEKKIYLGVTSGAVIYSSLPVERKYPIANFGGHNLRKIANNGKEYLFFVSYNKPENWEFRSDNSMVEDRRIVAGQ" }, "paralog": false, "identity": 0.9835164835164835, "score": 371.0, "evalue": 1.55e-133, "description": "Internal stop codon at 168050, 168401, 168458. Nonsense mutation around 168050, 168401, 168458.", "psc": { "uniref90_id": "UniRef90_A0A328EJD1", "uniref50_id": "UniRef50_A0A328EFX0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EJD1", "UniRef:UniRef50_A0A328EFX0" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EFX0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EFX0" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 13605.524199999987, "isoelectric_point": 5.186126136779785 }, "id": "NKOCPLAKPK_365", "locus": "GCA003260355_00328" }, { "type": "cds", "sequence": "contig_1", "start": 168643, "stop": 169002, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EFX0", "UniRef:UniRef90_A0A328EFX0" ], "nt": "ATGTTACTCTTGGGGATGTTTGCGATGTATAGCTTTCTTTCTCCCACGCTCCCTACTGAACCCGAGACTCCTCATATATGGGAGAGTGTTGACGGGCAGAAATTTACCCGGGAGGGCTACCCTGCTATCCCTGATAACACTGAAGTGCTTTGGTGGGACTGGAAGATTAAGTATCCTCCTGGGACAAAAAGGGCTTTTTTTGTGAAGGGAAAGCTGAAGAGAGAGGTATTCTCGTGTGAAGAGCTGAATGAATGTGAGTTTCCTCGATCAAAGCCTCGCACTGAACAGTGGTTGATTGTTCCCAAGGATGAGAAGTCTATCCGTGAAGATGAAGCAATTTATCTCGGCATGACTTCTTGA", "aa": "MLLLGMFAMYSFLSPTLPTEPETPHIWESVDGQKFTREGYPAIPDNTEVLWWDWKIKYPPGTKRAFFVKGKLKREVFSCEELNECEFPRSKPRTEQWLIVPKDEKSIREDEAIYLGMTS", "aa_hexdigest": "57b646527421de5c8043f7c8a6ca8bb3", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 168637, "stop": 169194, "observations": { "mutation": [ 168640, 169000, 169003, 169057 ], "stop": [ 168640, 169000, 169003, 169057 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EFX0", "query_cov": 1.0, "subject_cov": 0.6432432432432432, "identity": 1.0, "score": 255.0, "evalue": 2.23e-85, "gene_start": 1, "gene_end": 119, "reference_start": 3, "reference_end": 121, "reference_sequence": "MGMLLLGMFAMYSFLSPTLPTEPETPHIWESVDGQKFTREGYPAIPDNTEVLWWDWKIKYPPGTKRAFFVKGKLKREVFSCEELNECEFPRSKPRTEQWLIVPKDEKSIREDEAIYLGMTSGGTIYSSLPVEKGYPIANFGGYDLRKIANNEKEYLFFVSYNRLENWEFWSDSSVVEDRRIIAGQ" }, "paralog": false, "identity": 0.9783783783783784, "score": 377.0, "evalue": 1.01e-135, "description": "Internal stop codon at 168640, 169000, 169003, 169057. Nonsense mutation around 168640, 169000, 169003, 169057.", "psc": { "uniref90_id": "UniRef90_A0A328EFX0", "uniref50_id": "UniRef50_A0A328EFX0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EFX0", "UniRef:UniRef50_A0A328EFX0" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EFX0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EFX0" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 14005.952599999986, "isoelectric_point": 5.261778831481934 }, "id": "NKOCPLAKPK_366", "locus": "GCA003260355_00329" }, { "type": "cds", "sequence": "contig_1", "start": 169557, "stop": 169673, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAACATTATGAATTGGTGCTCTTGCTCAATGCAAACACTTCTGAAGCGGACAGGAAGGCGTTTTTAGAAGGTCTTGAATCGAAGTTTGAAGTAAAAGAAAAGGATGAAATCTGA", "aa": "MQHYELVLLLNANTSEADRKAFLEGLESKFEVKEKDEI", "aa_hexdigest": "ab661656b640e8911cbea6d5f509a7e1", "start_type": "ATG", "rbs_motif": "TTATT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4438.9622, "isoelectric_point": 4.677985572814941 }, "id": "NKOCPLAKPK_367", "locus": "GCA003260355_00330" }, { "type": "cds", "sequence": "contig_1", "start": 169758, "stop": 170114, "strand": "+", "frame": 3, "gene": "rpsR", "product": "30S ribosomal protein S18", "db_xrefs": [ "GO:0000314", "GO:0003735", "GO:0006412", "GO:0022627", "SO:0001217", "UniRef:UniRef50_A0A660MSR6", "UniRef:UniRef90_A0A660MS08" ], "nt": "GTGAAGGAAGTAAAGGCTGCTCTTTTGTATAACCAGGCACTTGTAAAGTATGAGATTTATAAGATGGGGAAAGATCAGAAATTTTTCCATTTTGAGAAGCTTCAGTCTGAGTTTGATAAAGCGATTGAAGAAATTAAGGAAAGAAAGTATGGACAGAAGATTAGTTTCTTTGCAAACGAGAGAAATGCAAAGTATATCAACTGGAAATCACTCCCTGTTTTGAAGTATTATCTTACGAGGTTTGGTGATATTAAGCCAAGAGCTTATACTGGAAACTCTGTAAAGATTCAGAAGAAAGTAAGGCAGGAGATTATCAGGGCAAGAACACTTGGATTGCTTTCCTTTATTAGTAGATAG", "aa": "MKEVKAALLYNQALVKYEIYKMGKDQKFFHFEKLQSEFDKAIEEIKERKYGQKISFFANERNAKYINWKSLPVLKYYLTRFGDIKPRAYTGNSVKIQKKVRQEIIRARTLGLLSFISR", "aa_hexdigest": "ac7b0a719245bf71000db5f0f2d4a444", "start_type": "GTG", "rbs_motif": null, "genes": [ "rpsR" ], "truncated": "5-prime", "pseudogene": { "start": 169557, "stop": 170114, "observations": { "mutation": [ 169671 ], "stop": [ 169671 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A660MS08", "query_cov": 1.0, "subject_cov": 0.6378378378378379, "identity": 0.992, "score": 227.0, "evalue": 1.99e-74, "gene_start": 1, "gene_end": 118, "reference_start": 68, "reference_end": 185, "reference_sequence": "MQHYELVLLLNANTSEADRKAFLEGLESKFEVKEKDEIGIQNLSFKLKDGNTKAYFVSYLLNLSPEQVKEVKAALLYNQALVKYEIYKMGKDQKFFHFEKLQSEFDKAIEEIKERKYGQKISFFANERNAKYINWKSLPVLKYYLTRFGDIKPRAYTGNSVKIQKKVRQEIIRARTLGLLSFISR" }, "paralog": false, "identity": 0.9945945945945946, "score": 361.0, "evalue": 1.68e-129, "description": "Internal stop codon at 169671. Nonsense mutation around 169671.", "psc": { "uniref90_id": "UniRef90_A0A660MS08", "gene": "rpsR", "product": "30S ribosomal protein S18", "uniref50_id": "UniRef50_A0A660MSR6", "go_ids": [ "GO:0000314", "GO:0003735", "GO:0006412", "GO:0022627" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A660MS08", "UniRef:UniRef50_A0A660MSR6", "GO:0000314", "GO:0003735", "GO:0006412", "GO:0022627" ] }, "pscc": { "uniref50_id": "UniRef50_A0A660MSR6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MSR6" ], "product": "Small ribosomal subunit protein bS6" } }, "id": "NKOCPLAKPK_368", "locus": "GCA003260355_00331" }, { "type": "cds", "sequence": "contig_1", "start": 170149, "stop": 170307, "strand": "+", "frame": 1, "gene": "rpmH", "product": "50S ribosomal protein L34", "db_xrefs": [ "GO:0000315", "GO:0003735", "GO:0006412", "GO:0022625", "KEGG:K02914", "SO:0001217", "UniParc:UPI000DBF7177", "UniRef:UniRef100_A0A328EHS9", "UniRef:UniRef50_A0A288DJ24", "UniRef:UniRef90_A0A7W4HL36" ], "nt": "ATGGCATCTAAATTAAGAAGAATTAGAAAATACCAGTGGACAAAGAGAATTAGGACTCATGGATTTAGAGAGAGAATGAATTCTTGGACTGGAAGGAGAGTTTTGGCTCGTAGAAGAGCTAAAGGAAGGCATCAATTGACTGTTCAAAGACAGAAATAG", "aa": "MASKLRRIRKYQWTKRIRTHGFRERMNSWTGRRVLARRRAKGRHQLTVQRQK", "aa_hexdigest": "f2e58f5391d1c360d2dd33dd8ffe1ff2", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000DBF7177", "uniref100_id": "UniRef100_A0A328EHS9", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBF7177", "UniRef:UniRef100_A0A328EHS9" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EHS9", "uniref90_id": "UniRef90_A0A7W4HL36" }, "psc": { "uniref90_id": "UniRef90_A0A7W4HL36", "gene": "rpmH", "product": "50S ribosomal protein L34", "uniref50_id": "UniRef50_A0A288DJ24", "kegg_orthology_id": "K02914", "go_ids": [ "GO:0000315", "GO:0003735", "GO:0006412", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_A0A288DJ24", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A288DJ24" ], "product": "Large ribosomal subunit protein bL34" }, "genes": [ "rpmH" ], "id": "NKOCPLAKPK_369", "locus": "GCA003260355_00332" }, { "type": "cds", "sequence": "contig_1", "start": 170496, "stop": 170786, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01156.24" ], "nt": "ATGGGTATGAAAGCGTTTAGCTCGGAAGAGAAAGTGCGTTTGCTTGTGGATTGTGATCCTGGTGCGGACGATGTATTTGCGTTGTTATGGTTGTTGATTCAGCATAAGTTTGCTGAGATTCCTTTGGAGATTGTCGGGATTACGACCGTTGGAGGGAATGTAGATGCAGACAAGACCTATGAGAATGTATTGCGTATTTGTGCAATGGTTGATGAGCATCGTATTCCTATAGGAAAAGATCATCGTCAAATTGTCTCTGAAGATGCTTCCTACATTCACGGGAATGATTGA", "aa": "MGMKAFSSEEKVRLLVDCDPGADDVFALLWLLIQHKFAEIPLEIVGITTVGGNVDADKTYENVLRICAMVDEHRIPIGKDHRQIVSEDASYIHGND", "aa_hexdigest": "d5e58a6ec10ce788c24ca259b865d804", "start_type": "ATG", "rbs_motif": "TTATT", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01156.24", "name": "IU_nuc_hydro", "length": 86, "aa_cov": 0.8541666666666666, "hmm_cov": 0.9186046511627907, "evalue": 2.1015019585390116e-19, "score": 66.7138442993164, "start": 15, "stop": 96 } ], "seq_stats": { "molecular_weight": 10695.095599999993, "isoelectric_point": 4.77051944732666 }, "id": "NKOCPLAKPK_370", "locus": "GCA003260355_00333" }, { "type": "cds", "sequence": "contig_1", "start": 170805, "stop": 170951, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01156.24" ], "nt": "ATGCTTCCTCCGGTGCAGTATCCTCAAGAAGTCCTTGACAGTGTAACGATGATTATTGAGGCGTTAGAGAAGTATCCTGATGAATTGACGATTATTGCTACGGGACCGTTGACGAATCTGGCACTTGCAGAAGAGAAAAATCCATGA", "aa": "MLPPVQYPQEVLDSVTMIIEALEKYPDELTIIATGPLTNLALAEEKNP", "aa_hexdigest": "60139af00447e113f7f0da87aacb8295", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01156.24", "name": "IU_nuc_hydro", "length": 43, "aa_cov": 0.875, "hmm_cov": 1.0, "evalue": 5.215282221720699e-10, "score": 35.871055603027344, "start": 7, "stop": 48 } ], "seq_stats": { "molecular_weight": 5310.0943, "isoelectric_point": 4.0500284194946286 }, "id": "NKOCPLAKPK_371", "locus": "GCA003260355_00334" }, { "type": "cds", "sequence": "contig_1", "start": 171090, "stop": 171353, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTGCTTCCGCTAGATCTCACTACAAGTATGCCTTTTACGATGAGGGATATGGAGAATTGTTTCATCTCCACCGTTAACAATACGCAGAAGCAGGAATTTATGAGGGCTTTGACGAAGTTTATTATTGCTACGAATATGAAGTTTAGAGAGACAGGTTATCAAGAAGGCTTTTATATCCACGATGCCCATACGGTAGGAATTTTGCTCTATCCTCATTTGTATAAAGGTTCATTTATGCAGGTTAATATTGAGACGCAATGA", "aa": "MLLPLDLTTSMPFTMRDMENCFISTVNNTQKQEFMRALTKFIIATNMKFRETGYQEGFYIHDAHTVGILLYPHLYKGSFMQVNIETQ", "aa_hexdigest": "8a1224f97aa745a18af6c355a0775cd6", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10203.749799999994, "isoelectric_point": 6.2465711593627935 }, "id": "NKOCPLAKPK_372", "locus": "GCA003260355_00335" }, { "type": "cds", "sequence": "contig_1", "start": 171525, "stop": 171758, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DC00F54", "UniRef:UniRef100_A0A328EG50", "UniRef:UniRef50_A0A328EG50", "UniRef:UniRef90_A0A328EG50" ], "nt": "ATGGAATTGGGACTTTTTGGTGCTGAAGGGGGACTTTTGTTTTACAAGGGAAAAGAAATCCTGATTCTGTTTTTTCTTTTTTTTGAAAATGGTCTTGATTTTTATCGGTATAACGCTATATACTTCTGTTGCATATTGGTAAATGGAATGGGATGGTGGTTTATTGGGCGTTTTGATTTGCCTTTTTGTTTTTCTTTGCGTGATAGGATCCCGATCACCTTTTGTGCAAAATAG", "aa": "MELGLFGAEGGLLFYKGKEILILFFLFFENGLDFYRYNAIYFCCILVNGMGWWFIGRFDLPFCFSLRDRIPITFCAK", "aa_hexdigest": "e0552776af9eca3732fb5fa82b55a9d4", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000DC00F54", "uniref100_id": "UniRef100_A0A328EG50", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DC00F54", "UniRef:UniRef100_A0A328EG50" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EG50", "uniref90_id": "UniRef90_A0A328EG50" }, "psc": { "uniref90_id": "UniRef90_A0A328EG50", "uniref50_id": "UniRef50_A0A328EG50" }, "pscc": { "uniref50_id": "UniRef50_A0A328EG50", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EG50" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9089.711399999991, "isoelectric_point": 6.098335075378416 }, "id": "NKOCPLAKPK_373", "locus": "GCA003260355_00336" }, { "type": "cds", "sequence": "contig_1", "start": 172017, "stop": 172325, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1W9VP59" ], "nt": "ATGATCGTGGAGACGAAAAATGTTGCTTTGAGGCCAGAAGTGCAGGAGCTCTTCAAAGACTATTATCAGCAGGATGGAAGAATCTTCCTTACCCAGCCAAGAAGCTATGGAGAATTGCCTGATCTTCTTGAACTTCAAAAGAAAGGGTATTCTGATTTTATCAATGTTTATCTTGGAAAACTTTTCTCTGATGTGGAGAATATCTGGGATATCGCTGGAGATAAGCTCTATGTAAATATTTCAGATTTGCAGGTTTCTGAACCAATTGAAACCGTAGATACTTGTAGGAAGAAGGAGCTTACCTACTGA", "aa": "MIVETKNVALRPEVQELFKDYYQQDGRIFLTQPRSYGELPDLLELQKKGYSDFINVYLGKLFSDVENIWDIAGDKLYVNISDLQVSEPIETVDTCRKKELTY", "aa_hexdigest": "5a0f91a893b0896ea1fd55f5e753979c", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A1W9VP59", "query_cov": 0.8725490196078431, "subject_cov": 0.8240740740740741, "identity": 0.573, "score": 121.0, "evalue": 6.82e-34, "valid": false, "uniref50_id": "UniRef50_A0A1W9VP59" }, "pscc": { "uniref50_id": "UniRef50_A0A1W9VP59", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1W9VP59" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11942.41259999999, "isoelectric_point": 4.575959587097168 }, "id": "NKOCPLAKPK_374", "locus": "GCA003260355_00337" }, { "type": "cds", "sequence": "contig_1", "start": 172353, "stop": 172604, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGAAAGAATTGAAGATGAGAAAACAAAGAAAGTATCTGAGAAAGTGCTTTTCAATAAGAGGGCAAATATCGGTATTCTTCCTTTGATGACGCCTTCAGCTTCTTATATTATCAATGGAGTTGAAAGAGTGATTATTTCTCAGATCATTAGATCATATGGTATCTTTTATGGAAAGAAAGATTTTTGGTATTCTTTCAAGCTTATTCCTGAGAACGGACCTTGGCTTGAAGTAAATGTAGAAAAAACCTGA", "aa": "MERIEDEKTKKVSEKVLFNKRANIGILPLMTPSASYIINGVERVIISQIIRSYGIFYGKKDFWYSFKLIPENGPWLEVNVEKT", "aa_hexdigest": "16f522a61112ad1d84ea9b93ae6d7fa3", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9681.193799999997, "isoelectric_point": 9.25922336578369 }, "id": "NKOCPLAKPK_375", "locus": "GCA003260355_00338" }, { "type": "cds", "sequence": "contig_1", "start": 172662, "stop": 174764, "strand": "+", "frame": 3, "gene": null, "product": "DNA-directed RNA polymerase subunit beta", "db_xrefs": [ "EC:2.7.7.6", "GO:0003677", "GO:0003899", "GO:0006351", "GO:0032549", "KEGG:K03043", "SO:0001217", "UniRef:UniRef50_A0A328EJ52", "UniRef:UniRef90_A0A328EJ52" ], "nt": "GTGTTCGGTCTAGAAACTGATGAAAGTATTAGAGAGGTATTTAACGGTATCTTGGAAGAGGAAGATGTTGATTTTATAGATATTACCTTGAAAAAAGACACAACCGTTGATGCGATTTCTGCGGCAGAGCATATCTATGGAAAACTCAGACCTGGAGAATTGATTGATGCGCAGTCAGCTTTGGATTATGTGAAAGGGCAGTTCTTACTCCCTGAGAGAATTTATGTTGGAAATATCGCAAGGAGAAAGATCAATGCAAAACTCAATCTTAAAAAGCCTTTGAAATCCGCTGAGGCAAATATTTTTGATGGAGCAGATCTTGTAGAAGCAGTGAAATACCTTGTGAATCTCTGTAATCAGAAAAAAGGTTTCTATACTGATGATTCTGATCATCTTTCCAACAAGAGAGTGAGAACGATGGGAGAAATCTTGTATTCTCACTTGCAACCTGTGATGAGAAAGTTTGTAAAGAGCGTGAGAGGAAAACTTTCCGTTCTCAATATGGAAAATCCGCTTAAAATCACTGATCTCGTAAACTTCAAGATGATTGATAACTCTATCAAATCGTTCTTTGCGACCAGTCAGCTTTCTCAATTTTTGGATCAGATCAATCCGCTTTCTGAAATTGAGCATAAGAGGAGAATTACCGCTCTTGGGCCTGGAGGTTTGAAGAGAGAAACTGCGAAATTTGAGGTGAGAGATGTGCATCCTTCGCATTATGGTAGAATTTGTCCGATTGAAACGCCAGAAGGACAGAATATTGGACTGGTAATTTACCAATCTCTCTATAGCAGAATCAATGATGAAGGATTCCTTGAGACACCTGCATTGAAAATTTTTCGTGAAGTAGAACCAAAAGCTGAATCGCTCGTTGGTAGAATCGCTCATCGTGATATTTATGAGCTTGATGCAAAGGGAAATCCTAGCAAGAAAGTGATTATCAAGGAAAATGAAGTGATTGATGAGAAAATCGCTGCCACTCTTGAAAAAGCCTATGGGAAACTCAAAAAACCGATCGCTGTAAAGCCGTTCTTTACTGGAGAAATTGAATATATCTCTCCAGAAATGGATGAAAAATGCGTAATTGCCGATGCGACCACTCCTTTGGATGAACATAATAATATCCTTTCTACGAGAGTTGCAGCAAGACACTTTAGTGAAATGGAGACCTTCCATGTGAACGATATTACGCATATGGATGTGAATTTGAGTCAGATTTTTTCTCCTAATACTTCTTTGATTCCTTTCGTAGATCATAACGATGCGGTGCGTGCTTCTGTAGCGACTAACCAGCAAAGACAGGCACTTCCGCTTCTCAAAAATGATGCTCCACTTGTTGGAACCGGACTTGAGAGAGATATCGTGAGAATGACGCATGCGGTGATTAAAGCTGAAGCTGATGGAGAAGTGATTTATGTGGATGGAGGAAGCGTTAAAGTAAAATATAAAGACGGAACGAAAGAATATCACTTGACGACTTTCGTGAGATCAAACCATAAAACTTGTATTACTCAGGTGCCTTGTGTAGATTTAGGACAGAAAGTGAAGAAAGGCGATTTGCTTGCAGAAGGACCTTGTTCTGTAAATGGAGAAATGGCAGTTGGAAAGTCGCTTAGAGTAGCTTTTATGCCATGGAAAGGGTATAACTACGAGGATGCGATCGTAATTTCTCAAAGACTGATCAAAGATGACGAATTGACTTCCATCATGATTAACGAATACGAGATTGAGGTTGCAGAGACCAAGCTCGGCCCTGAAGAAACAACGAATGATATTCCTGGAGTTGCGATGGCGAAACTAGGAAATCTTGATGAGGAAGGAATTATCAGGATTGGTTCTATTGTGAAAGGAGGGGATTTGCTTATTGGTAAGATCACTCCAAAGGGAGAAGGGGAGCTTACTCCAGAGGAAAAGCTGATTCAGGCGATCTTTGGAGATAAATCCAAGAATGTAAAAGATACTTCACTCTATATGCCTTCCGGAAGTGAAGGTAAGGTCGTAGATGTGGTGATCCTTGATGCGAAAAAAGGAGATAACCTCATGGCTGGAGTGAGGAAAAAGATCAAAGTTTATGTCGCTTCTACCAGAAAGATTGAAGTCTGA", "aa": "MFGLETDESIREVFNGILEEEDVDFIDITLKKDTTVDAISAAEHIYGKLRPGELIDAQSALDYVKGQFLLPERIYVGNIARRKINAKLNLKKPLKSAEANIFDGADLVEAVKYLVNLCNQKKGFYTDDSDHLSNKRVRTMGEILYSHLQPVMRKFVKSVRGKLSVLNMENPLKITDLVNFKMIDNSIKSFFATSQLSQFLDQINPLSEIEHKRRITALGPGGLKRETAKFEVRDVHPSHYGRICPIETPEGQNIGLVIYQSLYSRINDEGFLETPALKIFREVEPKAESLVGRIAHRDIYELDAKGNPSKKVIIKENEVIDEKIAATLEKAYGKLKKPIAVKPFFTGEIEYISPEMDEKCVIADATTPLDEHNNILSTRVAARHFSEMETFHVNDITHMDVNLSQIFSPNTSLIPFVDHNDAVRASVATNQQRQALPLLKNDAPLVGTGLERDIVRMTHAVIKAEADGEVIYVDGGSVKVKYKDGTKEYHLTTFVRSNHKTCITQVPCVDLGQKVKKGDLLAEGPCSVNGEMAVGKSLRVAFMPWKGYNYEDAIVISQRLIKDDELTSIMINEYEIEVAETKLGPEETTNDIPGVAMAKLGNLDEEGIIRIGSIVKGGDLLIGKITPKGEGELTPEEKLIQAIFGDKSKNVKDTSLYMPSGSEGKVVDVVILDAKKGDNLMAGVRKKIKVYVASTRKIEV", "aa_hexdigest": "ed18d5383edbc9116aa9c08f944702f8", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 172362, "stop": 175067, "observations": { "mutation": [ 172602, 174762, 174879 ], "stop": [ 172602, 174762, 174879 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EJ52", "query_cov": 1.0, "subject_cov": 0.5631536604987932, "identity": 0.9990000000000001, "score": 1366.0, "evalue": 0.0, "gene_start": 1, "gene_end": 700, "reference_start": 257, "reference_end": 956, "reference_sequence": "MVNKKRPGAEKAAKSSKSFSVVTKKTSATKGTVAKKANLSEMIVETKNVALRPEVQELFKDYYQQDGRIFLTQPRSYGELPDLLELQKKGYSDFINVYLGKLFSDVENIWDIAGDKLYVNISDLQVSEPIETVDTCRKKELTYGGIITAKVKLVERIEDEKTKKVSEKVLFNKRANIGILPLMTPSASYIINGVERVIISQIIRSYGIFYGKKDFWYSFKLIPENGPWLEVNVEKTGNIVARINKSRKFPITSLLRVFGLETDESIREVFNGILEEEDVDFIDITLKKDTTVDAISAAEHIYGKLRPGELIDAQSALDYVKGQFLLPERIYVGNIARRKINAKLNLKKPLKSAEANIFDGADLVEAVKYLVNLCNQKKGFYTDDSDHLSNKRVRTMGEILYSHLQPVMRKFVKSVRGKLSVLNMENPLKITDLVNFKMIDNSIKSFFATSQLSQFLDQINPLSEIEHKRRITALGPGGLKRETAKFEVRDVHPSHYGRICPIETPEGQNIGLVIYQSLYSRINDEGFLETPALKIFREVEPKAESLVGRIAHRDIYELDAKGNPSKKVIIKENEVIDEKIAATLEKAYGKLKKPIAVKPFFTGEIEYISPEMDEKCVIADATTPLDEHNNILSTRVAARHFSEMETFHVNDITHMDVNLSQIFSPNTSLIPFVDHNDAVRASVATNQQRQALPLLKNDAPLVGTGLERDIVRMTHAVIKAEADGEVIYVDGGSVKVKYKDGTKEYHLTTFVRSNHKTCITQVPCVDLGQKVKKGDLLAEGPCSVNGEMAVGKSLRVAFMPWKGYNYEDAIVISQRLIKDDELTSIMINEYEIEVAETKLGPEETTNDIPGVAMAKLGNLDEEGIIRIGSIVKGGDLLIGKITPKGEGELTPEEKLIQAIFGDKSKNVKDTSLYMPSGSEGKVVDVVILDAKKGDNLMAGVRKKIKVYVASTRKIEVGDKLAGKHGNKGIVAIVVPEENMPYSADGRPVDVVLNSLGVISRMNLGQLFESQLGLLAKELGVKFAVPTFGGFGVEDIVKLAQKAGLEDKLRTTLYDGETGEPYAQQVTVGYMHLLKLVHMVEDKIHARSVGPYSLITQQPLGGKSRQGGQRFGEMEVWALEAYSAVYTLQEMLTVKSDDVVGRNKLYESIIKSQKPRIGGLPESFNLLTYLFKGLGQNIVALTADELETIHQERTEKIKSLGLSGIMTASLEHETATDEETDESEDKGEMIGKVVEDLEDFGQIE" }, "paralog": false, "identity": 0.9966703662597114, "score": 1758.0, "evalue": 0.0, "description": "Internal stop codon at 172602, 174762, 174879. Nonsense mutation around 172602, 174762, 174879.", "psc": { "uniref90_id": "UniRef90_A0A328EJ52", "product": "DNA-directed RNA polymerase subunit beta", "ec_ids": [ "2.7.7.6" ], "uniref50_id": "UniRef50_A0A328EJ52", "kegg_orthology_id": "K03043", "go_ids": [ "GO:0003677", "GO:0003899", "GO:0006351", "GO:0032549" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EJ52", "EC:2.7.7.6", "UniRef:UniRef50_A0A328EJ52", "KEGG:K03043", "GO:0003677", "GO:0003899", "GO:0006351", "GO:0032549" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EJ52", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EJ52" ], "product": "DNA-directed RNA polymerase subunit beta" } }, "id": "NKOCPLAKPK_376", "locus": "GCA003260355_00339" }, { "type": "cds", "sequence": "contig_1", "start": 174894, "stop": 175364, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00562.33", "PFAM:PF04560.25" ], "nt": "ATGAACCTTGGTCAGCTTTTTGAATCTCAGTTGGGTTTGCTTGCGAAAGAACTTGGTGTGAAGTTTGCAGTGCCTACTTTTGGTGGATTTGGAGTAGAAGATATTGTGAAACTTGCTCAGAAAGCAGGACTAGAAGATAAGCTCAGAACGACGCTTTATGATGGAGAAACGGGAGAACCGTATGCTCAGCAGGTAACGGTTGGATATATGCATTTGCTCAAGCTCGTGCATATGGTAGAGGACAAAATCCATGCGAGATCTGTTGGTCCTTACTCTTTGATTACTCAGCAGCCACTTGGAGGAAAATCCAGACAAGGAGGACAGAGATTTGGAGAGATGGAAGTGTGGGCTTTGGAAGCATATTCTGCAGTGTATACCTTGCAAGAAATGCTTACGGTGAAATCTGATGATGTGGTCGGTAGAAATAAGCTTTATGAATCTATTATCAAATCTCAGAAACCAAGAATCTGA", "aa": "MNLGQLFESQLGLLAKELGVKFAVPTFGGFGVEDIVKLAQKAGLEDKLRTTLYDGETGEPYAQQVTVGYMHLLKLVHMVEDKIHARSVGPYSLITQQPLGGKSRQGGQRFGEMEVWALEAYSAVYTLQEMLTVKSDDVVGRNKLYESIIKSQKPRI", "aa_hexdigest": "30c3444c8a80c1f493172a3f07414926", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A1V5J6B2", "query_cov": 0.9743589743589743, "subject_cov": 0.8444444444444444, "identity": 0.618, "score": 197.0, "evalue": 8.18e-62, "valid": false, "product": "DNA-directed RNA polymerase", "uniref50_id": "UniRef50_X1FFN9" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00562.33", "name": "RNA_pol_Rpb2_6", "length": 106, "aa_cov": 0.6666666666666666, "hmm_cov": 1.0, "evalue": 2.0986722156312007e-32, "score": 109.08670806884766, "start": 1, "stop": 104 }, { "id": "PF04560.25", "name": "RNA_pol_Rpb2_7", "length": 54, "aa_cov": 0.28846153846153844, "hmm_cov": 1.0, "evalue": 3.8755256688223025e-18, "score": 62.1668815612793, "start": 106, "stop": 150 } ], "seq_stats": { "molecular_weight": 17319.77790000001, "isoelectric_point": 6.840481758117675 }, "id": "NKOCPLAKPK_377", "locus": "GCA003260355_00340" }, { "type": "cds", "sequence": "contig_1", "start": 175389, "stop": 175625, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGACCTATCTCTTCAAGGGATTGGGTCAGAATATTGTGGCTTTGACTGCTGATGAACTGGAAACGATTCATCAAGAGAGAACGGAAAAGATCAAAAGCCTTGGACTCTCAGGAATTATGACAGCTTCTCTTGAACATGAGACTGCAACTGATGAAGAAACTGATGAGTCTGAAGACAAAGGAGAAATGATTGGAAAAGTCGTTGAAGACCTTGAAGATTTTGGGCAGATTGAGTAA", "aa": "MTYLFKGLGQNIVALTADELETIHQERTEKIKSLGLSGIMTASLEHETATDEETDESEDKGEMIGKVVEDLEDFGQIE", "aa_hexdigest": "117376429d8248991602eb8fdae6fcd5", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8659.470999999998, "isoelectric_point": 4.217191886901856 }, "id": "NKOCPLAKPK_378", "locus": "GCA003260355_00341" }, { "type": "cds", "sequence": "contig_1", "start": 175753, "stop": 178365, "strand": "+", "frame": 1, "gene": "rpoC", "product": "DNA-directed RNA polymerase subunit beta'", "db_xrefs": [ "EC:2.7.7.6", "GO:0003677", "GO:0003899", "GO:0006351", "KEGG:K03046", "SO:0001217", "UniRef:UniRef50_A0A288GSY9", "UniRef:UniRef90_A0A660MU02" ], "nt": "ATGAACAAAACAAAATTTGATGGATTGATGGTAACTCTCGCTTCTATGGAGGAAGTCGCAAAATGGTCACATGGAGTGGTAGATAATCCAGATACCGTAAACTATAGGACAGGAAAGCCAAAACAAAGCGGACTTTTTTGTGAGTCTATTTTTGGTCCTGTGAAGAACTTTGAATGTAGCTGTGGAAAATATAAAGGAGTCAGATATAAGGGAATCGTCTGTGAAAGATGTGGAGTAGAAGTGACAAGTTCTAGAGAGAGAAGAGTGAGGATGGGACATGTAGATCTCGCTTCTCCAGTTGTGCATGTATGGTATAAAAACTCGCCATCCGGAGGTATTCACCAGCTCCTTCAGCTTTCAGCTAATGAAATAGATAAAATCCTGACTTTCGTGAAGTATGTTGTCGTAAAAGAGGTAGATGATGCTGTCAAAGATCAGATAAAAGAAAAGATCAGCACTGACTTTGAGGCAACCATGCAAGAACTTGATGAACTCTTCAAAAAAGAGACGGCTTCAGAAAAAGATACTAAGAAGCTTGCAGAGGCAAAAAAGCTTTATGAAGAGAATAAAGCTTCATTGGAAGCGGAATTTGGAAGAGTAAAATCGCTAGTAGCTGACCTTAAGTTTGGGGCGACGGTTTTGGAGTCTGACTACAGAAATGTTTTCAGTCAGTTTTCTAAACTGGTTTCTTTCGCTTCTGGACCTGAGGGAATCCTCAAAATGCTTCAAGCTATTGATGTAGAGAAGGAAATCAAAAAGAGAGTAAAGGAGTTTCCATCTATTAGATCGGCTGATCAGAAGAAAAAGGCAATGAGTCTAATTAAACTTTTGATCAATCTCTATGTATCAGGAGTGAAGCCAGAAAATATGATTATCAGAAAGCTTCCTGTGATCCCACCTGACATTAGACCAGTGGTGCAGCTTGATGGTGGAAGATTTGCTTCATCAGATGTGAATCTTTTCTATAGAAGGGTGCTGATGAGAAATATCAGACTCAAGAAGATGATCCAGGTTGGGATGCCTGATATTGTAAAAAAGAATGAAATCAGACTGCTTCAGGAGTCTATTAATAATCTTTTGGTGGGAGAAAAGAATGCTGCTGGTAAAGCAGGAGCAGGAATCAAAGTCTTCAAATCTATTTCCGATATGCTTTCTGGAAAGGAAGGAGTCTTTAGAAAGAACTTGCTCGGAAAGAGAGTGGATTATTCTGGTAGATCAATTATTACCGTAGGTCCAGACTTGAAACTCAGTGAATGTGGACTTCCGATCTATATTGCGGTGAAGATGTTTACGCCGTTTATTATCGGAAAGCTGATTGAAAAGAAGATCGTTTATACGCCAAAGCAAGCGGAAAAACTCATCAAAGAAGGAAATCCGACCGCTTTGAAATTCCTAGAAGAAGTGATTAAAGACAAATATGTGCTTCTTAACCGTGCACCAACGCTTCACAGACTTTCTATTGAAGCCTTCAAAATCAAGTTGATGCCTGGTAAAACAATCAGACTTCATCCTCTTGTTTGTCCTGCGTTTAATGCCGATTTTGATGGTGACCAAATGGCGGTGCATTTGCCTATTTCAGATGAAGCTCAAAAAGAAGCTCGCGAACTTATCGCAGCGGACAAGAATATTATCAAACCGGGTTCTGGAGAGCCAACGATTACGCACTCTCAAGATATGGTGCTTGGAATCTACTTCTTGACTGATTTTTTCAATCCAAAATATCCTGACTACAATACTGAAGAGGAATGGAAAGAAAAAATTCATCCTGTAGCTAGATTTGCTTCTTTTGATGAAGTGATGGCAGCGTTTAACAATAAACAAGTGCAACTCAAAGATAAAATCGTGGTGCTTGATGGTAATGAAGTGCTTGAAACTACGGTAGGAAGAGTGATCTTCAATTCTATTTTGCCAGAGAACTATCCGTATGTAAATAGAAAGATGGGAAATAAGGATCTCAAGAGGCTTTTGAGTGAGGTCTTTGACAAATACGATATGGAGACTACGGTAAAAGTTGCCGATGCGATCAAGGATTATGGATTTAAATATTCAACTATTGCTGCGACTTCAATCAATGTCCTTGACATGAAGGTTCCAAAGGAAAAAGAAGACCTTTTGAGGGCTGGTGATTTAGAAACTAATACGGTATTGAAATATTTCTATAAGGGATTCTTTTCTGAAGCAGAGAAACATAGATTGATCGTAAAAATCTGGACTGCAGTGAAAAAGGATGTAGAAGTGAACTTGAAATCTATTATTGGTGCAGGAAATAACCTCTATACGATGATTGATTCTGGAGCGAGAGGTTCTCAAACCCACCTTACGCAGATTTCAGGTATGAAGGGACTCGTAGTGAATCCACAGGGAGAAATTATTGAACTTCCGATTAAATCTTCTTTCGTAGAAGGATTGAAACCAATTGAATACTTTATTTCAGCTCACTCTGGTCGTAAAGGAAAGGCCGATACTGCCTTGAGAACTGCAGAATCTGGATACTTGACGAGAAAACTCTGTGATGCTTCCCAGGAAATGATCGTGAGAGAGGATGATTGTGGTTCTACAGAATCTATTCTCTATACAAGAGAGGAGGCTGAAATCAAAGGAGAAAAATTCTGA", "aa": "MNKTKFDGLMVTLASMEEVAKWSHGVVDNPDTVNYRTGKPKQSGLFCESIFGPVKNFECSCGKYKGVRYKGIVCERCGVEVTSSRERRVRMGHVDLASPVVHVWYKNSPSGGIHQLLQLSANEIDKILTFVKYVVVKEVDDAVKDQIKEKISTDFEATMQELDELFKKETASEKDTKKLAEAKKLYEENKASLEAEFGRVKSLVADLKFGATVLESDYRNVFSQFSKLVSFASGPEGILKMLQAIDVEKEIKKRVKEFPSIRSADQKKKAMSLIKLLINLYVSGVKPENMIIRKLPVIPPDIRPVVQLDGGRFASSDVNLFYRRVLMRNIRLKKMIQVGMPDIVKKNEIRLLQESINNLLVGEKNAAGKAGAGIKVFKSISDMLSGKEGVFRKNLLGKRVDYSGRSIITVGPDLKLSECGLPIYIAVKMFTPFIIGKLIEKKIVYTPKQAEKLIKEGNPTALKFLEEVIKDKYVLLNRAPTLHRLSIEAFKIKLMPGKTIRLHPLVCPAFNADFDGDQMAVHLPISDEAQKEARELIAADKNIIKPGSGEPTITHSQDMVLGIYFLTDFFNPKYPDYNTEEEWKEKIHPVARFASFDEVMAAFNNKQVQLKDKIVVLDGNEVLETTVGRVIFNSILPENYPYVNRKMGNKDLKRLLSEVFDKYDMETTVKVADAIKDYGFKYSTIAATSINVLDMKVPKEKEDLLRAGDLETNTVLKYFYKGFFSEAEKHRLIVKIWTAVKKDVEVNLKSIIGAGNNLYTMIDSGARGSQTHLTQISGMKGLVVNPQGEIIELPIKSSFVEGLKPIEYFISAHSGRKGKADTALRTAESGYLTRKLCDASQEMIVREDDCGSTESILYTREEAEIKGEKF", "aa_hexdigest": "a6e04fd4cb18cdccb1e5afd993a39182", "start_type": "ATG", "rbs_motif": "TTTTTA", "psc": { "uniref90_id": "UniRef90_A0A3D0UTS9", "query_cov": 0.8816091954022989, "subject_cov": 1.0, "identity": 0.737, "score": 1132.0, "evalue": 0.0, "valid": false, "product": "DNA-directed RNA polymerase subunit", "uniref50_id": "UniRef50_A0A3D0UTS9" }, "genes": [ "rpoC" ], "truncated": "3-prime", "pseudogene": { "start": 175753, "stop": 178668, "observations": { "mutation": [ 178363 ], "stop": [ 178363 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A660MU02", "query_cov": 1.0, "subject_cov": 0.6850393700787402, "identity": 0.997, "score": 1687.0, "evalue": 0.0, "gene_start": 1, "gene_end": 870, "reference_start": 1, "reference_end": 870, "reference_sequence": "MNKTKFDGLMVTLASMEEVAKWSHGVVDNPDTVNYRTGKPKQSGLFCESIFGPVKNFECSCGKYKGVRYKGIVCERCGVEVTSSRERRVRMGHVDLASPVVHVWYKNSPSGGIHQLLQLSANEIDKILTFVKYVVVKEVDDAVKDQIKEKISTDFEATMQELDELFKKETASEKDTKKLAEAKKLYEENKASLEAEFGRVKSLVADLKFGATVLESDYRNIFSQFSKLVSFASGPEGILKMLQAIDVEKEIKKRVKEFPSIRSADQKKKAMSLIKLLINLYVSGVKPENMIIRKLPVIPPDIRPVVQLDGGRFASSDVNLFYRRVLMRNIRLKKMIQVGMPDIVKKNEIRLLQESINNLLVGEKNAAGKAGAGIKVFKSISDMLSGKEGVFRKNLLGKRVDYSGRSIITVGPDLKLSECGLPIYIAVKMFTPFIIGKLIEKKIVYTPKQAEKLIKEGNPTALKFLEEVIKDKYVLLNRAPTLHRLSIEAFKIKLMPGKTIRLHPLVCPAFNADFDGDQMAVHLPISDEAQKEARELIAADKNIIKPGSGEPTITHSQDMVLGIYFLTDFFNPKYPDYNTEEEWKEKIYPVARFASFDEVMAAFNNKQVQLKDKIVVLDGNEVLETTVGRVIFNSILPENYPYVNRKMGNKDLKRLLSEVFDKYDMETTVKVADAIKDYGFKYSTIAATSINVLDMKVPKEKEDLLRAGDLETNTVLKYFYKGFFSEAEKHRLIVKIWTAVKKDVEVNLKSIIGAGNNLYTMIDSGARGSQTHLTQISGMKGLVVNPQGEIIELPIKSSFVEGLKPIEYFISAHSGRKGKADTALRTAESGYLTRKLCDASQEMIVREDDCGSTESILYTKEEAEIKGEKFGTLIYGRVLAEDAIDERGTVILRAGEMVTKSALSLIETSNITAVRVRSPLTCHCVSGVCQKCYGMDLATRNMVEVGVPVGVIAAQSIGEPSTQLTLDTFHEGGVAGSSEASGIDRVKQLFEVRPPKNPAGISPFDGTISFEETPEGGKFGKMKVTSEMQKKTYLVKSEYKVIVKEGADLAKGAVYASKGASKLKTKEAGKVLEVNEDYIVLGVQEIYTKSLVGLAPKKTKVGDKVYKGEILTTGALDVMEYKKIVGDIQAQRYIITEAKRVYAAQGQDLNDKHIEVVVKQLFSKVFIEDGGDSSFIPGTYVKYEEFIKVNRELQEAGKRPAQGQRVALGLTTVAKETDSWMSAASFQETIRVMVGASLRGAIDHLSDLKANVIIGRLLPVGENYRNMFGY" }, "paralog": false, "identity": 0.9948506694129763, "score": 1895.0, "evalue": 0.0, "description": "Internal stop codon at 178363. Nonsense mutation around 178363.", "psc": { "uniref90_id": "UniRef90_A0A660MU02", "gene": "rpoC", "product": "DNA-directed RNA polymerase subunit beta'", "ec_ids": [ "2.7.7.6" ], "uniref50_id": "UniRef50_A0A288GSY9", "kegg_orthology_id": "K03046", "go_ids": [ "GO:0003677", "GO:0003899", "GO:0006351" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A660MU02", "EC:2.7.7.6", "UniRef:UniRef50_A0A288GSY9", "KEGG:K03046", "GO:0003677", "GO:0003899", "GO:0006351" ] }, "pscc": { "uniref50_id": "UniRef50_A0A288GSY9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A288GSY9" ], "product": "DNA-directed RNA polymerase subunit" } }, "id": "NKOCPLAKPK_379", "locus": "GCA003260355_00342" }, { "type": "cds", "sequence": "contig_1", "start": 178384, "stop": 178929, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF04998.22" ], "nt": "GTGTTGGCTGAAGATGCGATTGATGAAAGAGGAACCGTGATATTGAGGGCAGGAGAAATGGTTACGAAATCAGCTTTAAGTCTGATTGAAACTTCAGGTATTACTGCTGTGAGAGTAAGGAGTCCATTGACTTGCCACTGCGTAAGTGGAGTGTGTCAGAAATGTTATGGAATGGACCTTGCTACGAGAAATATGGTAGAAGTAGGAGTGCCAGTTGGAGTAATCGCTGCGCAGTCTATTGGAGAACCATCTACTCAGCTTACGCTAGATACCTTCCATGAAGGAGGAGTGGCAGGTTCATCAGAGGCATCTGGTATTGATAGAGTGAAACAGCTCTTTGAGGTGAGACCACCAAAGAATCCTGCTGGAATTAGCCCATTTGATGGAACGATCTCTTTTGAAGAGACACCAGAGGGAGGAAAATTCGGAAAGATGAAGGTGACTTCTGAAATGCAAAAGAAAACTTACCTTGTGAAATCAGAGTATAAGGTTATTGTAAAAGAAGGAGCAGACCTTGTAAAAGGTGCTGTGTATGCGAGCAAATGA", "aa": "MLAEDAIDERGTVILRAGEMVTKSALSLIETSGITAVRVRSPLTCHCVSGVCQKCYGMDLATRNMVEVGVPVGVIAAQSIGEPSTQLTLDTFHEGGVAGSSEASGIDRVKQLFEVRPPKNPAGISPFDGTISFEETPEGGKFGKMKVTSEMQKKTYLVKSEYKVIVKEGADLVKGAVYASK", "aa_hexdigest": "b385cdbaffe1bedfbf2b730b1be108e1", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF04998.22", "name": "RNA_pol_Rpb1_5", "length": 63, "aa_cov": 0.34806629834254144, "hmm_cov": 0.9365079365079365, "evalue": 6.510615628131826e-20, "score": 68.1077880859375, "start": 53, "stop": 115 } ], "seq_stats": { "molecular_weight": 19214.915099999995, "isoelectric_point": 6.161994743347167 }, "id": "NKOCPLAKPK_380", "locus": "GCA003260355_00343" }, { "type": "cds", "sequence": "contig_1", "start": 179107, "stop": 179565, "strand": "+", "frame": 1, "gene": null, "product": "RNA polymerase Rpb1 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3D0UUB2" ], "nt": "ATGGAATACAAGAAAATCGTTGGAGATATTCAGGCTCAGAGATATATTATTACTGAAGCTAAGAGAGTGTATGCTGCGCAGGGACAAGACCTTAACGATAAGCATATCGAAGTCGTAGTGAAACAGCTCTTCTCAAAAGTCTTTATTGAAGATGGAGGAGATTCAAGTTTCATCCCAGGGACTTATGTGAAGTATGAAGAATTTATCAAAGTGAACCGTGAATTGCAGGAGGCTGGAAAGAGAGTTGCACAAGGACAGAGGGTTGCTCTTGGACTGACGACGGTTGCCAAGGAAACCGATTCTTGGATGTCGGCGGCTTCTTTTCAAGAGACGATTAGAGTGATGGTCGGTGCTTCGCTTAGGGGTGCGATTGATCATCTTTCAGATCTCAAGGCAAACGTTATTATCGGTAGGTTATTGCCGGTTGGGGAGAACTATAGGAATATGTTTGGTTATTAA", "aa": "MEYKKIVGDIQAQRYIITEAKRVYAAQGQDLNDKHIEVVVKQLFSKVFIEDGGDSSFIPGTYVKYEEFIKVNRELQEAGKRVAQGQRVALGLTTVAKETDSWMSAASFQETIRVMVGASLRGAIDHLSDLKANVIIGRLLPVGENYRNMFGY", "aa_hexdigest": "88a314226be33a207498967055f3ad42", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3D5YEX4", "query_cov": 0.9868421052631579, "subject_cov": 0.9554140127388535, "identity": 0.76, "score": 230.0, "evalue": 2.68e-75, "valid": false, "product": "RNA polymerase Rpb1 domain-containing protein", "uniref50_id": "UniRef50_A0A3D0UUB2" }, "pscc": { "uniref50_id": "UniRef50_A0A3D0UUB2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3D0UUB2" ], "product": "RNA polymerase Rpb1 domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_381", "locus": "GCA003260355_00344" }, { "type": "cds", "sequence": "contig_1", "start": 179929, "stop": 180084, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01693.22" ], "nt": "ATGGGGAAGCAAAAATGGTATGTGATATGGCAAGGAACGAAAACGGGGGTCTTCTCCGAATGGAAGGAAGTGGAAGGATTCGTAGCGGGGGTCAAGGGGGCGAAATACAAGGCGTTCGCGACGAAGCAAGAAGCAGAAGTGGCGTTGGGGAAATGA", "aa": "MGKQKWYVIWQGTKTGVFSEWKEVEGFVAGVKGAKYKAFATKQEAEVALGK", "aa_hexdigest": "5df04d27e44fc566dba78138abf8be91", "start_type": "ATG", "rbs_motif": "TTATT", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01693.22", "name": "Cauli_VI", "length": 42, "aa_cov": 0.8235294117647058, "hmm_cov": 1.0, "evalue": 1.2411797168872476e-15, "score": 53.918251037597656, "start": 5, "stop": 46 } ], "seq_stats": { "molecular_weight": 5696.533800000002, "isoelectric_point": 9.574538230895993 }, "id": "NKOCPLAKPK_382", "locus": "GCA003260355_00345" }, { "type": "cds", "sequence": "contig_1", "start": 180190, "stop": 180540, "strand": "+", "frame": 1, "gene": null, "product": "RNase H family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0027D7EBE8" ], "nt": "ATGGAATATCAAGGCATTGATTTAGCGACGGGGAAGCAAGTTTTTGCGGCTCGTTATGAGGAAGGGACGAATAATATTGGGGAGTTTTTGGCGATTGTGCATGGGCTCGCTTGGATTCAAAAAGAAAAGAAAGCTGACTATGTGCTTTATTCGGATTCCTTGATTGCGATGAAATGGGTCAATGAAGGGCGTTGCAAGACCTCGTTTATCGGGGCTGGGAATGGGAGCAGGATTTGGGAAGCGATCAAAAGAGCTGAGCAATGGTTGGCAGAGCATAAGTTTTCTACCCAGGTTTTGAAATGGCAAACAGAAGAATGGGGGGAGAATCCTGCGGACTATGGCAGGAAATAA", "aa": "MEYQGIDLATGKQVFAARYEEGTNNIGEFLAIVHGLAWIQKEKKADYVLYSDSLIAMKWVNEGRCKTSFIGAGNGSRIWEAIKRAEQWLAEHKFSTQVLKWQTEEWGENPADYGRK", "aa_hexdigest": "9ea034880b55afe3dc4fbbb8fa9c9bf7", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI003364CF09", "query_cov": 1.0, "subject_cov": 0.9133858267716536, "identity": 0.517, "score": 130.0, "evalue": 9.35e-37, "valid": false, "product": "RNase H family protein", "uniref50_id": "UniRef50_UPI0027D7EBE8" }, "pscc": { "uniref50_id": "UniRef50_UPI0027D7EBE8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0027D7EBE8" ], "product": "RNase H family protein" }, "genes": [], "id": "NKOCPLAKPK_383", "locus": "GCA003260355_00346" }, { "type": "cds", "sequence": "contig_1", "start": 180489, "stop": 180674, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAAACAGAAGAATGGGGGGAGAATCCTGCGGACTATGGCAGGAAATAAAAGTGATTTTGGGTATAAAAAGGGGAAAGGGGCGAGAAATTAGCCCTTTTTTGCGATCTTCTCTTGCAATTAGGGCTGAAAAGTGCATAGTCAGGGTAGCAGTCTTTTTTTATTCTTTTTTTAACTACCAATGA", "aa": "MANRRMGGESCGLWQEIKVILGIKRGKGREISPFLRSSLAIRAEKCIVRVAVFFYSFFNYQ", "aa_hexdigest": "c7c9a571bf71657dec44d5d92c6fd54d", "start_type": "ATG", "rbs_motif": "TTCTA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7029.248200000001, "isoelectric_point": 10.2894926071167 }, "id": "NKOCPLAKPK_384", "locus": "GCA003260355_00347" }, { "type": "cds", "sequence": "contig_1", "start": 180671, "stop": 180820, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAGTCAGATTTTTATGGTTTGGTGCGCTTTTGGTGCTGGCAGGAATGCTCTTTGCTGGGTGCGAGAAAGCTCAAGAAGAGAAGATTTCGATCAATACCAAGGATGCGATCAAGCTAGATCTTTTCCAATCTCAAGAGGAATGA", "aa": "MKKVRFLWFGALLVLAGMLFAGCEKAQEEKISINTKDAIKLDLFQSQEE", "aa_hexdigest": "7afdc481aed630614a90a9ec8b2e794f", "start_type": "ATG", "rbs_motif": "TTCTT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5589.5497, "isoelectric_point": 6.193767738342285 }, "id": "NKOCPLAKPK_385", "locus": "GCA003260355_00348" }, { "type": "cds", "sequence": "contig_1", "start": 180974, "stop": 182053, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTGCTCGCTATAAATGGATCAAGGTCTATGATATCCCTGAGGTAGCTGAGGATCAGGAATTGCTACAGTTTAATATCGCAAATTTGATGATCACCAAATATCCTTTGGTCATCAAGGACTATTACAAGCGTGGCAAGGAAGTGGGATTCATCAATCTCAAGACAGAAAAGCAAGGAAATGATTTGGTGATTCTTGGACCAAAGGTGAGCACGGGAGACAGCTATCTTTATGTAGATGTAAAGGAGCAAGGTATTGGTGGATTCAGATTTGAAAATAGTAATGAAGATCTGAAGCAAAGAAAGTCCTGGGAACATCCTCATTTTTTGAGGAGGGCAAGAGGAACATCTTTGTATAGTGGGCGTTGGTTGGTGAAGATATTGCAAGGGGGAGAGCGTTTTGACCGCGTCGTATTTCATTATCCATTAGGGGAGGTGCCACTTCAGCTCCCTGAATGGAATAATAATCCAGAGAACTGTGAAGTTGAGTTTAATGAGTATACGGTATTTACTGAATTGCCGCTCAGAGTCGTAGCTTCAGGGGTTGATTACAAGCTCGATTTTCATCCGGTGATTTCTGGAGCAGTGATAAAAAGTGCAAGGCTCCTCCAGCTTTCAGAAAAGCAGGCAAGTGGCTATCAATCTCTAGAAACACTCAAAGCATCGCTTGGTGAGAAGCAGGTCTTGATTGGAAAGCTTCCGTTTAATTACCAGGGATTTGCTGATAGAACGCAGAACGATCTAACTGATAAGACGGCATCAGATTTATTTCGTTTTAAGTTTTTTAAAGAGTGGACGGCTACGGTCAAAGCAGGCGTTCCTTCGCAAGTGGCGTTTACGATTCCTGAAGAAGGGAACTATCTTTTGCTCGTGGAGAATGATAATGGGCAGCTTTATGCTTTCAATTTTACGCCGAGAGCCGAGAATGCCCATGCTGTAGTCAAGAATGATAAAGAGGCAATGACGCTCACTATCGTGCATGATTGGCTTTTGACAGGGCAGGGGCTTTCTGGGCAGTTGGACCGCATCCTCGGAAGTGGGAACTATACGTTGGAGGTGAGTTCACTTAAAGAGGCGTGA", "aa": "MIARYKWIKVYDIPEVAEDQELLQFNIANLMITKYPLVIKDYYKRGKEVGFINLKTEKQGNDLVILGPKVSTGDSYLYVDVKEQGIGGFRFENSNEDLKQRKSWEHPHFLRRARGTSLYSGRWLVKILQGGERFDRVVFHYPLGEVPLQLPEWNNNPENCEVEFNEYTVFTELPLRVVASGVDYKLDFHPVISGAVIKSARLLQLSEKQASGYQSLETLKASLGEKQVLIGKLPFNYQGFADRTQNDLTDKTASDLFRFKFFKEWTATVKAGVPSQVAFTIPEEGNYLLLVENDNGQLYAFNFTPRAENAHAVVKNDKEAMTLTIVHDWLLTGQGLSGQLDRILGSGNYTLEVSSLKEA", "aa_hexdigest": "30018f8579aadfe6a878f47800c59199", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 40861.92540000004, "isoelectric_point": 5.967093849182128 }, "id": "NKOCPLAKPK_386", "locus": "GCA003260355_00349" }, { "type": "cds", "sequence": "contig_1", "start": 182075, "stop": 182662, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTATAGAGAGGAGGGGAAAGAGTATTATCCTGAAGGGCAGTATGTGAAGATCTCCTATTTTATTGCTCCTAATAAGCACTACACGGGGAATTTGGAAATTTTGGATCTTGACGGCAAGAAGTATAGTCATGCGGTAGATTTTTTTGTGGAGAAGATTGGTGATGCGAGGGTTTCTCGTGATTTGTTTTCTCAGAAGTTGAATGTATTACCGAGTGAAGGCTATCGAGCGGACAAGATAAAAGTAGGCTCTAAAAATGCCAGAGAACTCAAGATCTATACCCAAGTTTGTGAGGTAAAGCAAGAGGGGGATTTTTCTGATGCGAAAAGGAAGGAACTGCTTCCGCCTCGTAAATCGCATAATGAGTATGATGATTATGATTCTAGGACTACCTTAGGAGAGTTGCACTTTTTCGATTGCAAGGGGAAGATAGAAGAACACACGGTGCCGGTAGAGAAGTTTGAGTATCGGAAGAGGATTGATCGTGAGCGAGAGCTTCCGGCAAGTGTGAAAACGGTGCCGTATTTCCGTATGAGTTTCGATAAAGGCATGAAAGATGCGAAGTATTATATGAGGAGTCCCGTTTGA", "aa": "MYREEGKEYYPEGQYVKISYFIAPNKHYTGNLEILDLDGKKYSHAVDFFVEKIGDARVSRDLFSQKLNVLPSEGYRADKIKVGSKNARELKIYTQVCEVKQEGDFSDAKRKELLPPRKSHNEYDDYDSRTTLGELHFFDCKGKIEEHTVPVEKFEYRKRIDRERELPASVKTVPYFRMSFDKGMKDAKYYMRSPV", "aa_hexdigest": "b485c062dffed2573fd4a2d7bdeb0770", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 23024.817400000004, "isoelectric_point": 8.601968193054201 }, "id": "NKOCPLAKPK_387", "locus": "GCA003260355_00350" }, { "type": "cds", "sequence": "contig_1", "start": 182675, "stop": 182797, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGCGCCTTATCAGGAATTGTTGATCCGAGGGGTAAATTTTGCGGACGGGGCTGCGGTGCAGCAGGGAGAACTCCAGATTTTTGATGATAAGGGAAAACTGCTCAAGAAGGATCAGCTTTGA", "aa": "MAPYQELLIRGVNFADGAAVQQGELQIFDDKGKLLKKDQL", "aa_hexdigest": "30aa4aa34c9ff2cf2950451a9c568ad7", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4462.088, "isoelectric_point": 4.927338218688964 }, "id": "NKOCPLAKPK_388", "locus": "GCA003260355_00351" }, { "type": "cds", "sequence": "contig_1", "start": 182954, "stop": 184270, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01835.25" ], "nt": "ATGCCCGAACAGACTGATCAACAAATGGACTGGTATGAGAGGCGTGAGTCGCTTAAGATTTATGGTTATACAGACAGGGGGCTCTATAGACCTGGAGATACGGTGCATTTCGCCGGGTTTGTTCAGGATGTTGCGAAGGTGGCTAAGGATGGAACGAACGGAGTCCAGACAGGGTTTGTGATTGTTGAGATAACCGACTACGAAAGTGATGATAATAAGAAAATTGTTTTGTCCAAATTAGATGAATTTGGAGGGTTCGAGGGATCCTTTACCTTGAATGAGAGTAGCCCGCTTCGCTATTATCATATCAATTTTCATTATGCACTGGACAAGGATAAGATGAGCGTAGAAGATTTCGGTGGAGATTGGGAGGCTCGAGAGAATTATAGAAAGACGCATCCTGTGCTTTCTTATTGGTCAGATTTTCGTGTAGATGAGTTTCAAAAGCAGACCTTTAGTGTGCAAGTCAAAAAGGAGATCAAGGATAAGCAGCTTTATCTTGAATTGACGCCATCTTATTATATGGGAGGAGAATTAAAGGATTATGATCTGCATTTAACCTATTCCTTGGCGGCTAACCAATCATGCTCTGATTGTCGGTGGAGAGAAAGTAGCGATTACTACTATAACTATGTAGCGGATTCTTCTTTTTCTCGAGGCGGAAGCTATGAAAAGGAGCATCTCACTGGTGGTGCTGTTCTTTTCCAGATGGGGGATTTAGAGAAGCTCCCATATAAGGGAGAAAAGCTGAAGCTCCAAGTGCAGGCTTATGTTAAAGATAATCTTTCTGATGAGACCCGTATGAGTGTAGAGTATTTAGATATTGATCCAGAAGTGAAAATTGGGCTTAATGGCTATGCGTATGATTGGATTGATAGTAGTAGCTTGAAAAATTATCGTCTGACCTCAAGAATAGAGAGCAATCTCAATAAGGTTGCTGAACTTAGCTATGAATGGTATTTTAGACCGTTTGGGTTTGAGCAATCGGTTGATACGGAGGGAACTTTCTATTATATCAATGGAAGTAAGCTTCAGCTCGTTCAGTCTGGAACTTTACAACCAAAATCGGAAGTCAGCATTCCTTTGCTTTTTGAGCGCTATCAAGGAGGTGAGTATTTCCTTAGAGTTATTTCCAAGGACAAGTATGGCGTAGTCAATGGAGAAGTAGATAAGAATATCTATTACTACGATAGCAAGTTTGATAAAGGATTCCAAGGAGATATGGAGAATAACTATGCTCTTCATGTGCAGATTCCAAAGAAGAATTATGAGATTGGAGAAAACATTCCGATCAGTATCAATCCTTATATCAAATGA", "aa": "MPEQTDQQMDWYERRESLKIYGYTDRGLYRPGDTVHFAGFVQDVAKVAKDGTNGVQTGFVIVEITDYESDDNKKIVLSKLDEFGGFEGSFTLNESSPLRYYHINFHYALDKDKMSVEDFGGDWEARENYRKTHPVLSYWSDFRVDEFQKQTFSVQVKKEIKDKQLYLELTPSYYMGGELKDYDLHLTYSLAANQSCSDCRWRESSDYYYNYVADSSFSRGGSYEKEHLTGGAVLFQMGDLEKLPYKGEKLKLQVQAYVKDNLSDETRMSVEYLDIDPEVKIGLNGYAYDWIDSSSLKNYRLTSRIESNLNKVAELSYEWYFRPFGFEQSVDTEGTFYYINGSKLQLVQSGTLQPKSEVSIPLLFERYQGGEYFLRVISKDKYGVVNGEVDKNIYYYDSKFDKGFQGDMENNYALHVQIPKKNYEIGENIPISINPYIK", "aa_hexdigest": "c8817ab5d82111f6ab8ba5bbec114265", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01835.25", "name": "MG2", "length": 99, "aa_cov": 0.22602739726027396, "hmm_cov": 0.9292929292929293, "evalue": 1.8042665848104087e-16, "score": 56.77128982543945, "start": 19, "stop": 117 } ], "seq_stats": { "molecular_weight": 51001.10059999997, "isoelectric_point": 4.939842796325682 }, "id": "NKOCPLAKPK_389", "locus": "GCA003260355_00352" }, { "type": "cds", "sequence": "contig_1", "start": 184280, "stop": 184480, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTTACGGTAGAGAGAGGAAATCAGATCTTGGAGAAAAGGCTTGTTCGCCTAGATGGAAAGCCACTTACCTTGACTGCGAAAGAGGAGTTTTATCCTAATGTGCAGGTGAGTGTTACGGAGTTTGTAGGGGAGCAGGTTAATAGTCAGCTTGGAGGTAGTGGAGAACAAAAGAGAAAGGAGCCAGCTTTTTTTGCCTGA", "aa": "MLTVERGNQILEKRLVRLDGKPLTLTAKEEFYPNVQVSVTEFVGEQVNSQLGGSGEQKRKEPAFFA", "aa_hexdigest": "662d7a4a45699b77e444b37c910eb1e6", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7409.349600000002, "isoelectric_point": 6.218492698669433 }, "id": "NKOCPLAKPK_390", "locus": "GCA003260355_00353" }, { "type": "cds", "sequence": "contig_1", "start": 184568, "stop": 184792, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAAACTCAAGATTTTTACTACGGATTATCAAGGTAAGCCACTTACGGCGAGGGTTTCGCTGAGTGTCGTAGATAAAGCTTTGAGCGATCTTTATGATCTGATTAAGGATCCGATTCCTTACTTCTATAATAGAATTGGTAGCAGCATTGGGAACTTTGGAAACTGGAAGAACCTCTACTATGCCTTGAGGACCTTCTTTGCTGATGGAGAGAAAGGTGGATGA", "aa": "MKLKIFTTDYQGKPLTARVSLSVVDKALSDLYDLIKDPIPYFYNRIGSSIGNFGNWKNLYYALRTFFADGEKGG", "aa_hexdigest": "b61811bc95ad09ebeb26f25f5e879be2", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8403.530799999993, "isoelectric_point": 9.259674644470213 }, "id": "NKOCPLAKPK_391", "locus": "GCA003260355_00354" }, { "type": "cds", "sequence": "contig_1", "start": 184796, "stop": 185095, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATGGTTCTTTGAAGAATTTGAGGAAGAAGTTTTATGATCTTGCATTCCGAGATGCTGCGATTATTACTACGGGGGGAAAGGCTGAGCTTAGCTTTACCCTACCGGATAATCTCACGACTTGGATGCTCGATGCTGTTGCGGTTTCTCGTCAGAGTCAGCTCGGGACTTCAAGAGCTTACTTCAAAGTGAATCAACCTTTGGTAGTGGAGGCGAATTTGCCGACTTTCCTTACCATTGGAGATCAAATTACGCTTCCACTCAAACTGATTGCTTCTCCAGCAAAGCTTGCTCCATGA", "aa": "MDGSLKNLRKKFYDLAFRDAAIITTGGKAELSFTLPDNLTTWMLDAVAVSRQSQLGTSRAYFKVNQPLVVEANLPTFLTIGDQITLPLKLIASPAKLAP", "aa_hexdigest": "1033950170675a8ef77338455ad6ac99", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10809.471599999986, "isoelectric_point": 9.453724479675294 }, "id": "NKOCPLAKPK_392", "locus": "GCA003260355_00355" }, { "type": "cds", "sequence": "contig_1", "start": 185231, "stop": 186964, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGACGATTGAAGTCGAAGCCAAAGCAGGAAACTTTCACGATGCTGCTCAGTGGAAGATCCCGGTAAGGACTGAAGGGCTCGTAAATAAGAGCTTTTCACTTGAACAGCAGAGGAGCGGTGAACGTTTATTTACCTTTGATGATGAGGTGGTGAGGGGAAAACTCGTCGCTCGTCTGGCTCCGTTCCCAAGTTCTGTCTTGGCAGAACCGCTTCACTATTTACTCTTCTACTCTCCAAAGAGTCCTTCTGAGTGGCTCGTTAAGACGGCTCAAACTGCACTTGAGGCAGAAAGCTTGACTAAAGCAGGACTCTTGACCGGAGATTTCGTGCAGAGTGGAGCTATTCGTCTCGCAGAAGGTAAAAAGATGGCAATCTCTGAACTTTTAACGGCGGTGCTTTTCCAGCTCAAGGGCAGACAAGACTGGGATGGTGGAATCAAAGAACAAGATAGAAGTGATTATCCGTCTCGTTTTTGGGAGCAGAAGAATACTTCTTCACTCTTGACGAGTATCTCAACCTATGCCTTACTCGAGACGATGAAGACCAATGGATATGCCAACCTTGTGCCAAAAGATTTCTCAGAGAGGCTAGAGCGTTATCTTGCGAAGCTTGGAAGTGATTCCCCAGAACTTTATCTTTACTATCTCGCTCAGAGAAAGACTAACCTCGCATCAATCAATACCGCACTCGTTTCACAACTTCAGAAAAAGTATCCAAATAATATCGCTATTCAGGCTTTAAGCTTTTATCTGCTTGCACAGAATGCTGGAGAGGCAGATGCGATTCGCTTAGCTCAGAGTGTGGAGAAGCTTTTTGAGCAGGCGTTTGCTCAGGGACGCTATTTTGAAGGGGTGCCACTCACTTCGCTTGAGGAATTAGCTCCTGAATTGCTTGCTTTGGCAGAGGTAGCCGGCAATCGTAATGCTCATTTATCGCTAGATCTCAAAGCTTTACAAGGGTACTATCTGAGAGGAGTTCTCAAACTCCATACTAGATTCCCAAAGCAAGAGCATTTTACGAACCAGATCTCGAGTCAGATTCTTTCTCTTCTCAAGTCCAGAAGTGCGTATGGGCTCTGGAGCTGGTCGTCTACGACAAACTTTGCTGTCTTGAGTGCTCTCAATGCTGCACTCACCTCTTACTATCAGCATCAAAGTCAGACAACTCATTGTGTGCTCCAGGTGGGGAATACGAAGCTCGAGCTTGATGCGGGGAAGACTTGTGTAGAAAAGAGTATTCCGTTTGAGCATACCAAGGATCTGAAGGTGCAACGGTCCTGTGAAGGTCCTGCCTTCCTTGATCTCCAAGTCAACTCTTTACTCAAAGATATTACCAAGAAGAAGACCGAGCTTCACAATGTGGAAAAACTCACGTTTGAGTATCCGACAACGGCTCAAATCGGTGAAAAGATTCCTCTTAAGGCGGGATTCAAAACGCTCAAAGAGGCTCAGAATTTGACTGTTGAGTTTACGATCCCTTCCAGATTTAAATTCTGGTCCAATCTCCAAGTGGCAGAGAATAGCTTTGAGAAGGGAATCTTTGACTTTGGAAATGATCGAGCGTGCTTTCCGGACTTTTATGAGTTTAGGTTTGATCGTGTAATCCTTAACTATGGCACGATGAAGGCTGGTAAGGAATGTAACTTTACCTTTTATGCGGTGAATGCGTTTGATGGGCAGTTCGTCTACCCATCGAGCAAGCTTTATGAACAGCAGGCTACACAAGTATGGTGA", "aa": "MTIEVEAKAGNFHDAAQWKIPVRTEGLVNKSFSLEQQRSGERLFTFDDEVVRGKLVARLAPFPSSVLAEPLHYLLFYSPKSPSEWLVKTAQTALEAESLTKAGLLTGDFVQSGAIRLAEGKKMAISELLTAVLFQLKGRQDWDGGIKEQDRSDYPSRFWEQKNTSSLLTSISTYALLETMKTNGYANLVPKDFSERLERYLAKLGSDSPELYLYYLAQRKTNLASINTALVSQLQKKYPNNIAIQALSFYLLAQNAGEADAIRLAQSVEKLFEQAFAQGRYFEGVPLTSLEELAPELLALAEVAGNRNAHLSLDLKALQGYYLRGVLKLHTRFPKQEHFTNQISSQILSLLKSRSAYGLWSWSSTTNFAVLSALNAALTSYYQHQSQTTHCVLQVGNTKLELDAGKTCVEKSIPFEHTKDLKVQRSCEGPAFLDLQVNSLLKDITKKKTELHNVEKLTFEYPTTAQIGEKIPLKAGFKTLKEAQNLTVEFTIPSRFKFWSNLQVAENSFEKGIFDFGNDRACFPDFYEFRFDRVILNYGTMKAGKECNFTFYAVNAFDGQFVYPSSKLYEQQATQVW", "aa_hexdigest": "e48f0241bf3ee786197ca1e36378ece2", "start_type": "GTG", "rbs_motif": "TTCTT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 65118.27460000019, "isoelectric_point": 8.030907249450681 }, "id": "NKOCPLAKPK_393", "locus": "GCA003260355_00356" }, { "type": "cds", "sequence": "contig_1", "start": 187123, "stop": 187512, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBFA747", "UniRef:UniRef100_A0A328EJY7", "UniRef:UniRef50_A0A328EJY7", "UniRef:UniRef90_A0A328EJY7" ], "nt": "ATGAAGAACAAATGGAAATGGATGATGGTTGCTGGTCTTTTGCTTGTGGTGGCTGGAGGTATTTTCTTTTGGATTCGTAGTGATCGTGCAGCTACGAGGCGTGAAATGATCCTAAAAGATAGAGCATGTCTCCTAGGCTATACCAAGTTTCTCCATTGTTTTATAGGGGATACTTTGCAGGGAACTCAGGAGTGTAAGGACTCTTTCACTCCAGAAGAAGAAGCTTTTGTAACTCAATGTTCTTCTTACTTTATACAGAACTATAAATTGAGTAAATGTTTTCGTAAGGGGGATAAGAAATTAGCATCTCTGGCTGAACTTCCTTCAGCAGATCAGTATTCAACCTTGTATACCTACGGATGTATGCTCTCTACCTTGTATGATTTTTAG", "aa": "MKNKWKWMMVAGLLLVVAGGIFFWIRSDRAATRREMILKDRACLLGYTKFLHCFIGDTLQGTQECKDSFTPEEEAFVTQCSSYFIQNYKLSKCFRKGDKKLASLAELPSADQYSTLYTYGCMLSTLYDF", "aa_hexdigest": "16b62ba83f1165bd6804c4c0f57b1305", "start_type": "ATG", "rbs_motif": "TTATT", "ups": { "uniparc_id": "UPI000DBFA747", "uniref100_id": "UniRef100_A0A328EJY7", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBFA747", "UniRef:UniRef100_A0A328EJY7" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EJY7", "uniref90_id": "UniRef90_A0A328EJY7" }, "psc": { "uniref90_id": "UniRef90_A0A328EJY7", "uniref50_id": "UniRef50_A0A328EJY7" }, "pscc": { "uniref50_id": "UniRef50_A0A328EJY7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EJY7" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14895.203499999985, "isoelectric_point": 8.730711555480958 }, "id": "NKOCPLAKPK_394", "locus": "GCA003260355_00357" }, { "type": "cds", "sequence": "contig_1", "start": 187615, "stop": 187848, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGGCGTGGGGGGAGGCACACTCAGCAAGCAGTCGACATCCTGTGCAACTCCTTCTGGTATGTGTTGCAAACCCTTGTTAGCGTAGCTGGATTTAGGTTTGCAGGGCTTGCAAGTGGTCGGAATGGAGCATTTTTGGGGTTTGCAAGACGTGCAAGTGGTCGGAAAGGATCATTTTTGGGGTTTGCAAGACGTGCAAGTGGTCAGAATGGAGCATTTTTGGGGCTTGCATGA", "aa": "MRRGGRHTQQAVDILCNSFWYVLQTLVSVAGFRFAGLASGRNGAFLGFARRASGRKGSFLGFARRASGQNGAFLGLA", "aa_hexdigest": "07e35c8657453e5ee54317861d76c9c0", "start_type": "ATG", "rbs_motif": "TTTA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8239.346099999995, "isoelectric_point": 11.999967765808105 }, "id": "NKOCPLAKPK_395", "locus": "GCA003260355_00358" }, { "type": "cds", "sequence": "contig_1", "start": 188562, "stop": 188789, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATTTCACAAGTTATCTGGCAAAAAAAGGCGAAGTATGGAGATCAAGTGATTATTTTACCTCAAGAAGGAGAGATTCCTTTGGATGAGTACATTAGAGCATCACAACAAGCTCGTGTGCAAGAAGGAAGACTCCCGAGTTTTATGATGGAATATCCTCGTTTTTCTTCCCTTCTTCGTGATGCAGGGTTTGTGATTGAGGCGGTTTCTGGAGGGATTCCCTTCTGA", "aa": "MISQVIWQKKAKYGDQVIILPQEGEIPLDEYIRASQQARVQEGRLPSFMMEYPRFSSLLRDAGFVIEAVSGGIPF", "aa_hexdigest": "99946dc24dfccafeb21e02765fc5f18f", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8530.764899999996, "isoelectric_point": 5.25041103363037 }, "id": "NKOCPLAKPK_396", "locus": "GCA003260355_00359" }, { "type": "cds", "sequence": "contig_1", "start": 188817, "stop": 189077, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATCGAGTTGCCGTCTTTCCCGATTGAGACGAAGCCGTATGCAATCTATGCTGAGGGAGAGAGAGAAGGTGTTGGAGGAGAAGGGTTCCGTTATCCGTGTGTGGCGTATCTTAGAAGTGCGACTATGAACTCTGGGGTAGATCCTCGTTGTTTACAGGTTCTGATTAATAGGCAGGGGGACGATCTTCGTCTGTATTTCGATGCGTTTACGGGAACGGTGCTCAAAGTAAATCCAGTGGCTTCTCCGTTAGAGCATTAG", "aa": "MIELPSFPIETKPYAIYAEGEREGVGGEGFRYPCVAYLRSATMNSGVDPRCLQVLINRQGDDLRLYFDAFTGTVLKVNPVASPLEH", "aa_hexdigest": "77f720461506f6a465fd095be5950059", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9551.783399999997, "isoelectric_point": 4.967807579040527 }, "id": "NKOCPLAKPK_397", "locus": "GCA003260355_00360" }, { "type": "cds", "sequence": "contig_1", "start": 189349, "stop": 190047, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00912.28" ], "nt": "GTGCTGGGGAATTTTTGCTATAGGGTGCCGCTTCCAGAGCTTGAGAGGCAGGTCCTCAGAGGGATCAACGGGGAGCTTCTCCAAGGGCTGAGGGAGATTCCGTATGCGAGTCCTGAAGAAGTGCCTGAAAATCTCAAGCGTGCGGTCGTGCGTGTGGAAGATCGTAGGTTTTATTGGCATCTAGGGGTTGATCCGATTGCGATTTTGAGGGCGATGCGGTTCAATTTGACGAGTATTGGGAAGGTGCAAGGGGCGTCCACCTTGCCTCAGCAACTTGTGAAACTCACTGAAAAGGCGTATCACAGGAGCTGGAAGCAGAAGCTTTGGGAGATGGGGCTTGCGTTCAATCTCTCTTTACATTATTCCAAGCAGGAGCTCCTCTTGGCGTATCTGAATCAGGTAGAGTTTCTCAATGGCGTGCGTGGCTACAGGTCTGCGTGTTTGAGTTATTTTGGGAAGGAATGTGGAGCGCTGTTTCCTTCAGAGCTGAGTTTTTTGATTGCAACGGCTCAGACAGGGAAGAATCCCTTGAGGCAAGGGGATTTTCAGCTCATAAAAGAGAAAGCTGAAGCTCTCTGTCAGGTGGCCTTTAGCAAAGAGCAATGCGATAGATGGGAAGAGCTTCCTCCGCTATCAGCAAAAGCGTTGCTTCTCCAGCCTAACCGTTCGGTTTTTCAGTTTTCTGACTATTATTTATGA", "aa": "MLGNFCYRVPLPELERQVLRGINGELLQGLREIPYASPEEVPENLKRAVVRVEDRRFYWHLGVDPIAILRAMRFNLTSIGKVQGASTLPQQLVKLTEKAYHRSWKQKLWEMGLAFNLSLHYSKQELLLAYLNQVEFLNGVRGYRSACLSYFGKECGALFPSELSFLIATAQTGKNPLRQGDFQLIKEKAEALCQVAFSKEQCDRWEELPPLSAKALLLQPNRSVFQFSDYYL", "aa_hexdigest": "5ed488ab57f24e9d992fabe35631104a", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00912.28", "name": "Transgly", "length": 144, "aa_cov": 0.6120689655172413, "hmm_cov": 1.0, "evalue": 1.1354038453796685e-41, "score": 138.61170959472656, "start": 35, "stop": 176 } ], "seq_stats": { "molecular_weight": 26675.57480000001, "isoelectric_point": 8.91489772796631 }, "id": "NKOCPLAKPK_398", "locus": "GCA003260355_00361" }, { "type": "cds", "sequence": "contig_1", "start": 190090, "stop": 190542, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00905.27" ], "nt": "TTGTATGAGCGTATTGAGCAGGTGATTTCCTCTAGTGAGCATTATAGGCAGCAGATGCAGATGGAGGATTGTTGTGTATTGGTATTGGATGCGGCGGGTGAGGTGCGTAGCATGAATACGTGTAGACCGTTTTATGAGAAAGAGCAAGGGATGGTCAATGGCTGTCTGAGTAAGAGGCAAACCGGATCTGCGATTAAGCCGTTCCTTTATTTGTTTGCGATGAATGCATTGCAGTATACGGGAGGGACGAAGATTGTGGATGAGCCGGTCTCTTACTATTTAGATGAAGAGCATATTTATAGTCCGAAGAATTTTTCGCTTTCCTATCATGGAGAAGTATCGCTTGCAGATGCGTTGGGGAATAGTTTTAATGTCCCTGCGGTGAAGCTCTTGCACGAAGTCGGGATTGAGGAGTTTATTGCATTTATCAATCAGCTGAGGCTCCGTTTCTGA", "aa": "MYERIEQVISSSEHYRQQMQMEDCCVLVLDAAGEVRSMNTCRPFYEKEQGMVNGCLSKRQTGSAIKPFLYLFAMNALQYTGGTKIVDEPVSYYLDEEHIYSPKNFSLSYHGEVSLADALGNSFNVPAVKLLHEVGIEEFIAFINQLRLRF", "aa_hexdigest": "e6eab0662025b8a6d21b2b35267bbf0b", "start_type": "TTG", "rbs_motif": "TTCTT", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00905.27", "name": "Transpeptidase", "length": 126, "aa_cov": 0.8133333333333334, "hmm_cov": 0.9920634920634921, "evalue": 2.3016035192032605e-10, "score": 36.58522415161133, "start": 25, "stop": 146 } ], "seq_stats": { "molecular_weight": 17149.377999999997, "isoelectric_point": 5.180442237854003 }, "id": "NKOCPLAKPK_399", "locus": "GCA003260355_00362" }, { "type": "cds", "sequence": "contig_1", "start": 190597, "stop": 190764, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGGGCTTTCGGCGGCGCTTGGAACCTATGAGCTTTCTCCGTTAGAGTTTGCACAGTTTTGGAGGGTTTTCTTGGCGGGAGCTGAGGAGGGGCTTGTTGCTTCCAAGGTTATTGATGAGGTATTTGCTATTTTGTCTGAGAATCGTCATAGGGTGGTCAGTTTTTGA", "aa": "MGLSAALGTYELSPLEFAQFWRVFLAGAEEGLVASKVIDEVFAILSENRHRVVSF", "aa_hexdigest": "95bc270969812992aa629004d7c0a16c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6072.895799999998, "isoelectric_point": 4.796722221374512 }, "id": "NKOCPLAKPK_400", "locus": "GCA003260355_00363" }, { "type": "cds", "sequence": "contig_1", "start": 190781, "stop": 190909, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTGGCGTGATGGGCAGTTAAATCAGGCACTTCTAGGCATTTCGTCGATGGTTGGACCTGTGGTGCGAATCGTGAGAAGCAGCGTATTGTCTGTGTATGGGCGGGAAACTATGATGCTACTCCTATGA", "aa": "MWRDGQLNQALLGISSMVGPVVRIVRSSVLSVYGRETMMLLL", "aa_hexdigest": "9f1500525321a3cda3fbe803b7620039", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4677.580699999998, "isoelectric_point": 10.666697120666505 }, "id": "NKOCPLAKPK_401", "locus": "GCA003260355_00364" }, { "type": "cds", "sequence": "contig_1", "start": 191182, "stop": 192093, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00133.28", "PFAM:PF13603.12" ], "nt": "ATGTTAGAGAAGTATAGTCCAAATGAGATAGAAAACAAGCGAGGAAAACTTTGGGAAGAAAAAGGTTATTTTAGAGCGACAACTGATGCTGAGAAGGCGAATTTCAGTATTGTGATTCCTCCTCCAAATGTGACTGGAATCCTCCATATGGGACATGTGCTGGATGAAGCGATCCAAGATGTATTGGTGAGGTGGAAAAGAATGTCTGGATTCAATACTCTACGAATGCCAGGAACCGATCACGCAGGAATCGCTACTCAAAACAAGGTAGAAAGAGCTTTAGCTGAAGAAGGGAAGACAAAAGAAGACCTTGGAAGAGATGAATTTATCAAAAGAACTTGGGAGCGAAAAGAAAAACACGGTGGGATCATCACTCAACAACAGAGAAAACTTGGAGCATCACTCGATTGGGAAAAGGAAAGATTCACGATGGATGAAGGGCTTTCTGCGGCAGTGAGAGAGATTTTCGTGAAACTTTACCATGATAATCTGATTTATCAGGGGGAATATATGGTAAACCGATGTCCAAGGTGCGGAACTGCACTTGCTGATGATGAGGTGGAACATGTGGATTCTGAAGGGCATTTTTGGGAGATTGCCTATCCGATCGTGGGAACTGATGAAAAGCTGATCCTTGCGACCACGAGACCTGAAACGATGCTCGGAGATACGGGAGTTGCAGTGCATCCTGAGGATGAAAGGTATCAACATTTGATCGGGAAGAAAGTGCTTTTGCCACTGATGGATAGAGAAATCCCTATCGTTGCCGATGAATATGTAGATAGAGAGTTTGGAACTGGAGTGGTGAAGATGACTCCTGCTCATGATCCAAATGATTTTGAGGTGGGAAAGAGGACGGGACTGGAAATTATCAACATTTTCACTCCTGATGGAAAAGTAAATGAAAACTGA", "aa": "MLEKYSPNEIENKRGKLWEEKGYFRATTDAEKANFSIVIPPPNVTGILHMGHVLDEAIQDVLVRWKRMSGFNTLRMPGTDHAGIATQNKVERALAEEGKTKEDLGRDEFIKRTWERKEKHGGIITQQQRKLGASLDWEKERFTMDEGLSAAVREIFVKLYHDNLIYQGEYMVNRCPRCGTALADDEVEHVDSEGHFWEIAYPIVGTDEKLILATTRPETMLGDTGVAVHPEDERYQHLIGKKVLLPLMDREIPIVADEYVDREFGTGVVKMTPAHDPNDFEVGKRTGLEIINIFTPDGKVNEN", "aa_hexdigest": "ccfad733c4d5ac48e792979ba6928927", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00260C5F76", "query_cov": 0.8844884488448845, "subject_cov": 0.8874172185430463, "identity": 0.705, "score": 400.0, "evalue": 7.89e-138, "valid": false, "product": "class I tRNA ligase family protein", "uniref50_id": "UniRef50_A0A2V7YJ46" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00133.28", "name": "tRNA-synt_1", "length": 181, "aa_cov": 0.5874587458745875, "hmm_cov": 1.0, "evalue": 3.0146235220251568e-61, "score": 203.81735229492188, "start": 16, "stop": 193 }, { "id": "PF13603.12", "name": "tRNA-synt_1_2", "length": 61, "aa_cov": 0.20132013201320131, "hmm_cov": 1.0, "evalue": 2.296564900798819e-13, "score": 46.48977279663086, "start": 239, "stop": 299 } ], "seq_stats": { "molecular_weight": 34531.72030000002, "isoelectric_point": 5.263086128234862 }, "id": "NKOCPLAKPK_402", "locus": "GCA003260355_00365" }, { "type": "cds", "sequence": "contig_1", "start": 192109, "stop": 193434, "strand": "+", "frame": 1, "gene": null, "product": "valine--tRNA ligase", "db_xrefs": [ "EC:6.1.1.9", "GO:0000166", "GO:0004812", "GO:0004832", "GO:0005524", "GO:0006438", "KEGG:K01873", "SO:0001217", "UniRef:UniRef50_A0A1V6ADP5", "UniRef:UniRef90_A0A328EFI1" ], "nt": "TTGGATCGTTTTGAGGCGAGAAAACAGATCGTAGCTGATTTGGAGGCTGGGGGGTATCTCGTGCTAGTGAGGAAGCATCAGCATGCCGTTGGGCATTGTTATAGGTGTGATACCATTGTAGAACCTCGCGTTTCTAAGCAGTGGTTTGTAAAGATGAAACCTCTCGCAGAACCAGCACTTGAAGCGGTAAAATCTGGAAAAATCAAAATTCAGCCTGAAAGATGGGTGAAAGTGTATTATCATTGGCTGGAAAATATCAGAGATTGGTGTATTTCTAGGCAGATTTGGCGAGGGCACAGAATCCCTGCTTGGTATGGACCTGATGGGAAGGTTTTCGTGGCAAAAAATGAATCTGAAGCGAAAAGTCAGGCTTTAGCTTTTTATGGGAAAGAAGTGAGTTTGGAGCAGGAAAATGATGTGCTTGATACTCGGTTTTCTTCTGCACTTTGGCCATTTTCAACGATGGGACGACCAGAAAAAACTGAACTTTTGGAACATTTCTATCCGACTTCAACCCTCGTAACGGGAGCAGACATCATCTTTTTTCGAGTAGCAAGAATGGTAATGTTTGGACTGTATGAGATGAAAGATGTGCCGTTTCACGATGTGTTTTTCCATGGAATCGTGAGAGATGAGCTGGGAAGGAAAATGTCCAAATCGCTTGGAAATTCGCCTGATCCTTTGAAACTCATTGATGAATATGGAGCAGATGCGATCAGATTTACGATGATTTACAATACTTCCGCTGGTCAGGATGTGCATTTCTCTGAAAAGCTCATTGAAATGGGGAGAAACTTTGCAAATAAGGTTTGGAATGCGACCAATTTCGTTTTGATGAATTTGGAAGGCTTTGATCCAAAACAGGTGAAAAAATCAGATTTGGCTTTTGAATTGGTAGATCAGTGGATTTATTCAAGACTGAATACTGCGATTGCGGAGACGGAAAGCTATTTGAATAAATATGCTCTTGATGAGGCGGCGAAAGTCGTATATGAGTTTATCAGGGGAGATTTCTGCGATTGGTATGTAGAAATGGCAAAGGTGAGGCTCTATATGAATGATGACATTCAGAGTAAGCAAACTGCACAATGGGTGCTTTGGGAGGTGCTAGAAACCGCTTTGAAACTGCTTCATCCGTTTATGCCGTTCATCACTGAGGAGCTGTGGCAACATGTGAAAACTGAGGGAGAAACGATTATGCTCCAGTCTTTTCCAAAAAGTGAAAAAAATCTGATTTCTGCTGAGGTGGAAACTGCGATGGCTTGGCTCCAAGAGGCGATTACTGCCTTTAGAAATATCAGAGCTGAAGCGAATATCAATCCATGA", "aa": "MDRFEARKQIVADLEAGGYLVLVRKHQHAVGHCYRCDTIVEPRVSKQWFVKMKPLAEPALEAVKSGKIKIQPERWVKVYYHWLENIRDWCISRQIWRGHRIPAWYGPDGKVFVAKNESEAKSQALAFYGKEVSLEQENDVLDTRFSSALWPFSTMGRPEKTELLEHFYPTSTLVTGADIIFFRVARMVMFGLYEMKDVPFHDVFFHGIVRDELGRKMSKSLGNSPDPLKLIDEYGADAIRFTMIYNTSAGQDVHFSEKLIEMGRNFANKVWNATNFVLMNLEGFDPKQVKKSDLAFELVDQWIYSRLNTAIAETESYLNKYALDEAAKVVYEFIRGDFCDWYVEMAKVRLYMNDDIQSKQTAQWVLWEVLETALKLLHPFMPFITEELWQHVKTEGETIMLQSFPKSEKNLISAEVETAMAWLQEAITAFRNIRAEANINP", "aa_hexdigest": "24adc8c5d5ce0e0635177852b6ef5309", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 191809, "stop": 193737, "observations": { "mutation": [ 192091, 192094, 193432 ], "stop": [ 192091, 192094, 193432 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EFI1", "query_cov": 1.0, "subject_cov": 0.498868778280543, "identity": 0.998, "score": 904.0, "evalue": 0.0, "gene_start": 1, "gene_end": 441, "reference_start": 310, "reference_end": 750, "reference_sequence": "MLEKYSPNEIENKRGKLWEEKGYFRATTDAEKANFSIVIPPPNVTGILHMGHVLDEAIQDVLVRWKRMSGFNTLRMPGTDHAGIATQNKVERALAEEGKTKEDLGRDEFIKRTWERKEKHGGIITQQQRKLGASLDWEKERFTMDEGLSAAVREIFVKLYHDNLIYQGEYMVNRCPRCGTALADDEVEHVDSEGHFWEIAYPIVGTDEKLILATTRPETMLGDTGVAVHPEDERYQHLIGKKVLLPLMDREIPIVADEYVDREFGTGVVKMTPAHDPNDFEVGKRTGLEIINIFTPDGKVNENGGKYAGLDRFEARKQIVADLEAGGYLVLVRKHQHAVGHCYRCDTIVEPRVSKQWFVKMKPLAEPALEAVKSGKIKIQPERWVKVYYHWLENIRDWCISRQIWRGHRIPAWYGPDGKVFVAKNESEAKSQALAFYGKEVSLEQENDVLDTRFSSALWPFSTMGRPEKTELLEHFYPTSTLVTGADIIFFRVARMVMFGLYEMKDVPFHDVFFHGIVRDELGRKMSKSLGNSPDPLKLIDEYGADAIRFTMIYNTSAGQDVHFSEKLIEMGRNFANKVWNATNFVLMNLEGFDPKQVKKSDLAFELVDQWIYSRLNTAIAETESYLNKYALDEAAKVVYEFIRGDFCDWYVEMAKVRLYMNDDIQSKQTAQWVLWEVLETALKLLHPFMPFITEELWQHVKTEGETIMLQSFPKSEKNLISAEVETAMAWLQEAITAFRNIRAEANINPGKAVPALIKTADPEEMKILTQNKAFLMKLAKLESLDFGAEIVKPELSGVRVVGKSELIVPLLGLLDLEAEKEKIMAQIAKLEQGLASCERKLADERFVSKAPAQVIEREKANQADYQHKIEKLKENLAHLGGSI" }, "paralog": false, "identity": 0.9953271028037384, "score": 1282.0, "evalue": 0.0, "description": "Internal stop codon at 192091, 192094, 193432. Nonsense mutation around 192091, 192094, 193432.", "psc": { "uniref90_id": "UniRef90_A0A328EFI1", "product": "valine--tRNA ligase", "ec_ids": [ "6.1.1.9" ], "uniref50_id": "UniRef50_A0A1V6ADP5", "kegg_orthology_id": "K01873", "go_ids": [ "GO:0000166", "GO:0004812", "GO:0004832", "GO:0005524", "GO:0006438" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EFI1", "EC:6.1.1.9", "UniRef:UniRef50_A0A1V6ADP5", "KEGG:K01873", "GO:0000166", "GO:0004812", "GO:0004832", "GO:0005524", "GO:0006438" ] }, "pscc": { "uniref50_id": "UniRef50_A0A1V6ADP5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1V6ADP5" ], "product": "Valine--tRNA ligase" } }, "id": "NKOCPLAKPK_403", "locus": "GCA003260355_00366" }, { "type": "cds", "sequence": "contig_1", "start": 193450, "stop": 193836, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF10458.14" ], "nt": "TTGATCAAAACTGCCGATCCAGAGGAAATGAAGATTTTGACTCAAAATAAAGCCTTTTTGATGAAATTGGCGAAATTGGAAAGCTTGGACTTTGGTGCAGAAATCGTGAAACCTGAACTTTCAGGAGTAAGGGTCGTTGGAAAGTCAGAATTGATCGTGCCTTTGCTTGGATTGCTTGATCTGGAAGCAGAAAAGGAAAAGATTATGGCTCAGATTGCCAAGCTTGAACAGGGATTAGCTTCTTGTGAGAGGAAATTGGCTGATGAGAGGTTTGTTTCCAAGGCTCCTGCACAAGTGATTGAGAGAGAAAAGGCAAATCAAGCAGATTATCAGCATAAGATTGAAAAGTTGAAAGAGAATTTAGCTCATTTAGGTGGTTCTATTTAG", "aa": "MIKTADPEEMKILTQNKAFLMKLAKLESLDFGAEIVKPELSGVRVVGKSELIVPLLGLLDLEAEKEKIMAQIAKLEQGLASCERKLADERFVSKAPAQVIEREKANQADYQHKIEKLKENLAHLGGSI", "aa_hexdigest": "f55552647c3e9f45f9a9cd7a9c44ca8a", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF10458.14", "name": "Val_tRNA-synt_C", "length": 66, "aa_cov": 0.515625, "hmm_cov": 1.0, "evalue": 2.1323056308849703e-17, "score": 59.62314224243164, "start": 60, "stop": 125 } ], "seq_stats": { "molecular_weight": 14209.513299999984, "isoelectric_point": 6.1658029556274405 }, "id": "NKOCPLAKPK_404", "locus": "GCA003260355_00367" }, { "type": "cds", "sequence": "contig_1", "start": 194085, "stop": 194321, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGTTTCCAATTTGGATATTTGATGTGGTTTGTTTGATAATAAGAGTTCCTATTAAGGCAGATAGAAACCAAGTAATTCGTAATATTATTTTTCTTATTTCCCTCGTGCAGTTAAACTTGTTTTTTAACGATTCATCTACCCGAGAAAGAATGGCAATGAATATTATAACTGTACATGTAAAATATCAAGCTATGATCATGCTGTCATTAATCCATGTAGATGCATCAAAATAA", "aa": "MLFPIWIFDVVCLIIRVPIKADRNQVIRNIIFLISLVQLNLFFNDSSTRERMAMNIITVHVKYQAMIMLSLIHVDASK", "aa_hexdigest": "56c572a8a3b48284e97727e765820bd3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9131.972399999995, "isoelectric_point": 9.619472694396972 }, "id": "NKOCPLAKPK_405", "locus": "GCA003260355_00368" }, { "type": "cds", "sequence": "contig_1", "start": 194960, "stop": 195295, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGGGTTTTGGTGGGAGGAAGACTTGGGCATGTGTTGATCTATGGAGAAGGCTATTATTTCGCTCATTTAACGGAGATTTTTAAGGTTTGGGAAGGTGGAATGTCCTTTATTGGAGGGATTTTCGGGGTCGTGATCAGTATTTTTTTGCTTTGTCTGATGAAAAGAGTTTCCAAGAAGGAATTTTTGGTTCTTTTTGATTTGATGCTTATTTTCGTTCCTTTGGGGATTTTTTTTGGGAGGTTTGGGAATTTTCTTAATCAAGAATTGTATGGAATCCCGCTTGCGGAGCTTCCTGATCGATTAGGTCAGTTTTTTTCTTCTTTAGGGCTTTGA", "aa": "MGVLVGGRLGHVLIYGEGYYFAHLTEIFKVWEGGMSFIGGIFGVVISIFLLCLMKRVSKKEFLVLFDLMLIFVPLGIFFGRFGNFLNQELYGIPLAELPDRLGQFFSSLGL", "aa_hexdigest": "b091bc688e32a0b808de4e02fb0d9669", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12446.766799999983, "isoelectric_point": 6.712878227233887 }, "id": "NKOCPLAKPK_406", "locus": "GCA003260355_00369" }, { "type": "cds", "sequence": "contig_1", "start": 195628, "stop": 195894, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF09365.15" ], "nt": "ATGTTTAGTATCACTCTTAAATTTTTGGAAGCGGTGAAGCGTAATAACTCGACAGAGTGGTTGCATGCTCATCGGGATCTCTATCAGCAGGAAAAGAAGAGATTTTCTCAGTTTGTAGAGGCTTTTTTGAGGGAAGCAAAAAGTATGCATCCGATATTGGAGGATTTACAGGTCAAGGATTGTATTTATAGGTTTAATAGGGATTTGCGTTTCTCTAAAGATGGGAAGCCGTATAAGGAGAACTTTGGAGCGATCTTCGCTTACTGA", "aa": "MFSITLKFLEAVKRNNSTEWLHAHRDLYQQEKKRFSQFVEAFLREAKSMHPILEDLQVKDCIYRFNRDLRFSKDGKPYKENFGAIFAY", "aa_hexdigest": "6fb7a42d792b31945785edb1c831b03e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF09365.15", "name": "DUF2461", "length": 82, "aa_cov": 0.9318181818181818, "hmm_cov": 1.0, "evalue": 4.238115825123222e-28, "score": 95.1059799194336, "start": 5, "stop": 86 } ], "seq_stats": { "molecular_weight": 10681.126699999986, "isoelectric_point": 9.334071159362793 }, "id": "NKOCPLAKPK_407", "locus": "GCA003260355_00370" }, { "type": "cds", "sequence": "contig_1", "start": 196594, "stop": 196881, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTGAAGTCAGACTTCAGTCTTTGCAATAATCATGAGTCCATCAGTATCCTTATCCAGCCTATGCAGCAGTCCAGCTCTGATAAAATTCCCAATGCTTGGAAGCTCTTTGTAATGATGATAAAGAAAGCCAACGACACTCGGCTTGCCAACCTCCCAAATACTGTTGGGATGGGACAAAACTCATTTCGGTTTATTAATAACCAGATAATCAGTGGTTTCCCTCACGATCGGAATCTCAATAGAAGGTGCTTCCTCAAGAATCACAGGAGACAAATATCTCTCTAG", "aa": "MLKSDFSLCNNHESISILIQPMQQSSSDKIPNAWKLFVMMIKKANDTRLANLPNTVGMGQNSFRFINNQIISGFPHDRNLNRRCFLKNHRRQISL", "aa_hexdigest": "a0084c883d93456a59cdba4d7a337d8a", "start_type": "ATG", "rbs_motif": "TTTA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11031.683399999987, "isoelectric_point": 10.595201683044433 }, "id": "NKOCPLAKPK_408", "locus": "GCA003260355_00371" }, { "type": "cds", "sequence": "contig_1", "start": 197044, "stop": 197313, "strand": "+", "frame": 1, "gene": null, "product": "Pseudouridine synthase RsuA/RluA-like domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4IF45" ], "nt": "GTGAGAGCGAAAGTCCCTAATGCGGTTCCAAAGCTCGGAATCACGCAGATTGAAAAAATAGAAAAAAAACCTGATGGGAGGATTCAGCTTTTTCTCCAGATTTTTACTGGGAGGACGCATCAGATCAGATTTCATCTGAGTAATCACGGTTTGCCGATTGTTGGGGATTATCTCTATGGGAAGGAAAGTCAGGAAGCGATGCAACTTACCGCTTATCAGCTGGAATTTGAGGATTTAGGAGGAGAGAGGGTAACAATAAAAATTGATTAG", "aa": "MRAKVPNAVPKLGITQIEKIEKKPDGRIQLFLQIFTGRTHQIRFHLSNHGLPIVGDYLYGKESQEAMQLTAYQLEFEDLGGERVTIKID", "aa_hexdigest": "ed91aa8042fd4acf9a9aa0e57d69a10f", "start_type": "GTG", "rbs_motif": "TTATT", "psc": { "uniref90_id": "UniRef90_A0A2W4IF45", "query_cov": 0.8314606741573034, "subject_cov": 0.8131868131868132, "identity": 0.851, "score": 133.0, "evalue": 4.62e-39, "valid": false, "product": "Pseudouridine synthase RsuA/RluA-like domain-containing protein", "uniref50_id": "UniRef50_A0A2W4IF45" }, "pscc": { "uniref50_id": "UniRef50_A0A2W4IF45", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4IF45" ], "product": "Pseudouridine synthase RsuA/RluA-like domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_409", "locus": "GCA003260355_00372" }, { "type": "cds", "sequence": "contig_1", "start": 197499, "stop": 198089, "strand": "+", "frame": 3, "gene": null, "product": "DNA damage-inducible protein D", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7X7BME0" ], "nt": "GTGATCAAAAAGGCGGAAATTGCCTGTAAAAAAAGTGGTCAGGCTACGACAAATCACTTCAGCGAATTTACGGAAAAAGCAAAAATTGGTTCTGGTGCGACGAGGGATTTAAAAAGCTGGAAATTGAGTCGTTATGCTTGCTATCTCATTATTCAGAATGCAGATCCGAATAAGGAAATAGTAGCTCTTGGGCAAAGTTATTTTGCATTGCAGACAAGGCAGCAGGAGCTTTCTGCTCAGTATCTCGAGGATCAGAAAAGGCTTGAGCTCAGAGAGGAGGTCACCAAGCACAATAAGAAGCTTTTTAGCACGGCGAGGGGAGCTGGAGTTTATGACTATGCGACTTTTTATGATTCTGGGTATCAAGGGCTTTATGGGCTCAAAAAGAAAGAAATTATAGAAAAGAAAAATCTGAATCCAGATGAGAATATGCTTGACCATATGAGTAGTGAGGAATTGGCTGCGAATCTTTTCCGTGCGACTCAAGCTGAAGCAAAGATTAAAAGAGAAGCCATCAGAGGACAAGAAAAGGCGAGTCAAGCTCATTTTCAAGTAGGACAAAAAATCAGAGCCACCATCAAAGAGCTCTGA", "aa": "MIKKAEIACKKSGQATTNHFSEFTEKAKIGSGATRDLKSWKLSRYACYLIIQNADPNKEIVALGQSYFALQTRQQELSAQYLEDQKRLELREEVTKHNKKLFSTARGAGVYDYATFYDSGYQGLYGLKKKEIIEKKNLNPDENMLDHMSSEELAANLFRATQAEAKIKREAIRGQEKASQAHFQVGQKIRATIKEL", "aa_hexdigest": "c223162d1e74ac1273fbdf5dc8989a3f", "start_type": "GTG", "rbs_motif": "TTTA", "psc": { "uniref90_id": "UniRef90_A0A932QVH2", "query_cov": 1.0510204081632653, "subject_cov": 0.8306451612903226, "identity": 0.5579999999999999, "score": 204.0, "evalue": 4.21e-63, "valid": false, "gene": "dinD", "product": "DNA damage-inducible protein D", "uniref50_id": "UniRef50_A0A7X7BME0", "kegg_orthology_id": "K14623" }, "pscc": { "uniref50_id": "UniRef50_A0A7X7BME0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7X7BME0" ], "product": "DNA damage-inducible protein D" }, "genes": [], "id": "NKOCPLAKPK_410", "locus": "GCA003260355_00373" }, { "type": "cds", "sequence": "contig_1", "start": 198096, "stop": 198389, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCGGAAGATATTCCAGCGGTGGAAGACATCAAATTTGCGAGGAAAAGGTTAGAGGAGCTAGAAGTCCAAGATGCACAGCAGATGCCGAGTTTCGCTCAGCAGCTTGGTGAAAAGTTAGAAGAGCAAGAAATGGTCAAGTATGAGAAATTGCCCGATAATATTCAGCTTTTGGAAGCTTTGGCAAAGATTATCAGAGGTTATCCTGGAGAGAGACTTCTGCTTTTGGGAGAGAAGAAATTTAAGCTTTCCAGCGAGGGGATGAGATTGGTGAAGGGGCTTTTAAATGGATAG", "aa": "MPEDIPAVEDIKFARKRLEELEVQDAQQMPSFAQQLGEKLEEQEMVKYEKLPDNIQLLEALAKIIRGYPGERLLLLGEKKFKLSSEGMRLVKGLLNG", "aa_hexdigest": "f53a9a244132018473b07197b9c71f59", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11111.84179999999, "isoelectric_point": 5.082906532287596 }, "id": "NKOCPLAKPK_411", "locus": "GCA003260355_00374" }, { "type": "cds", "sequence": "contig_1", "start": 198464, "stop": 199018, "strand": "+", "frame": 2, "gene": null, "product": "Elongation factor P", "db_xrefs": [ "KEGG:K02356", "SO:0001217", "UniParc:UPI000CCA3475", "UniRef:UniRef100_A0A2N8N501", "UniRef:UniRef50_A0A2N8N501", "UniRef:UniRef90_A0A2N8N501" ], "nt": "ATGGTAAAAATTGATGCGGCAGAAGTAAAGAAAGGAACTGTGCTTGAGATGGATGGACAGCTTTTTAAGGTGCTTGATCTTTCTTTTATGCAGATGCAGCAAAGGCAAGGGTCTTATACTTATAAGGTAAGAAATCTGATGACTAATGGAGTACAAAATGTGACTGTAAAGTCCGGAACTACCATGGATCAGGCAGATATTTCTACTCAAAATGCAGTTTATCTCTATAATACAGGAGATACTTATTCTTTCATGGAGAACGATACTGGAGAGATTCATGAAATTGCAGCAGATACGATTGAAGATATTACCGGATATCTAAAAGATAATCTTGATGTCTTTATTCAGATCTACAAAGGGAATGTTATCAATGTGATCCTTCCTTCTACGATCAGCTATACGATTACTGAAACCGTTCCTGGAATTAAGGGGAATAGAGCTCAGTCTGGAACTAAGCCTGCTACCATTGAAACTGGAATGGAAATTCAGGTACCACTTCACAAAGAAGTAGGAGATACCGTGACTTTGAATACGCTTACTGGAGGTGTAAACTAG", "aa": "MVKIDAAEVKKGTVLEMDGQLFKVLDLSFMQMQQRQGSYTYKVRNLMTNGVQNVTVKSGTTMDQADISTQNAVYLYNTGDTYSFMENDTGEIHEIAADTIEDITGYLKDNLDVFIQIYKGNVINVILPSTISYTITETVPGIKGNRAQSGTKPATIETGMEIQVPLHKEVGDTVTLNTLTGGVN", "aa_hexdigest": "a50c4993b71909b109043fb6be162617", "start_type": "ATG", "rbs_motif": "TTTTT", "ups": { "uniparc_id": "UPI000CCA3475", "uniref100_id": "UniRef100_A0A2N8N501", "db_xrefs": [ "SO:0001217", "UniParc:UPI000CCA3475", "UniRef:UniRef100_A0A2N8N501" ] }, "ips": { "uniref100_id": "UniRef100_A0A2N8N501", "uniref90_id": "UniRef90_A0A2N8N501" }, "psc": { "uniref90_id": "UniRef90_A0A2N8N501", "product": "Elongation factor P", "uniref50_id": "UniRef50_A0A2N8N501", "kegg_orthology_id": "K02356" }, "pscc": { "uniref50_id": "UniRef50_A0A2N8N501", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2N8N501" ], "product": "Elongation factor P" }, "genes": [], "id": "NKOCPLAKPK_412", "locus": "GCA003260355_00375" }, { "type": "cds", "sequence": "contig_1", "start": 199082, "stop": 199192, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAAAAATTCTCTTTGTCGTTGCGAATGTAGGGTTTCAGGATGAAGAATTTAGTATCCCTTTTGAACTCTTATCTTCTCAAGGGCATTTTTGTGACGTTGTTTCTTGA", "aa": "MAKILFVVANVGFQDEEFSIPFELLSSQGHFCDVVS", "aa_hexdigest": "73763db562ebe4b8e3b42a278b56098a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4004.5384000000013, "isoelectric_point": 4.1722322463989245 }, "id": "NKOCPLAKPK_413", "locus": "GCA003260355_00376" }, { "type": "cds", "sequence": "contig_1", "start": 199272, "stop": 199508, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTATTGTGGTGTTTGTTGGTTGAGGTGGTGCTTATGAGGAGTATTTTCAGAATGCGAGCTATCTTGACCTTGCTCGTCAGGCGAAAGCGATTGCTGCGATTTGTATTGCTCCAACTTTACTTTCTGATTCTGGTCTTTTACAGGGGAAACACGTTACCGGTTGGGATGATGGTTTCTGAACGCAGATCGGTTATTTACAGCATAATGGGGCTATTTTTAAAGATGAAGAAGTAG", "aa": "MLLWCLLVEVVLMRSIFRMRAILTLLVRRKRLLRFVLLQLYFLILVFYRGNTLPVGMMVSERRSVIYSIMGLFLKMKK", "aa_hexdigest": "c79e7be8edc2b9c6511fe5b0ce7e8def", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9410.802499999994, "isoelectric_point": 11.662862586975098 }, "id": "NKOCPLAKPK_414", "locus": "GCA003260355_00377" }, { "type": "cds", "sequence": "contig_1", "start": 199508, "stop": 199597, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCAAGATGGAAATCTGATTACTGCGAATGGTCCAGCTGCCGCGACGAAATTTGCTTGGGTGATTGTTGATTTTCTTAAAAATTCTTGA", "aa": "MQDGNLITANGPAAATKFAWVIVDFLKNS", "aa_hexdigest": "15addc4945abc4da31b0d083093aae92", "start_type": "GTG", "rbs_motif": "TTTA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3093.5099999999993, "isoelectric_point": 5.713648796081543 }, "id": "NKOCPLAKPK_415", "locus": "GCA003260355_00378" }, { "type": "cds", "sequence": "contig_1", "start": 200383, "stop": 200580, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAGCAGAGAATATCCTGAACATAAAACCTCGACTTTCTCCTACGAAAAATTTTCTACCTATCTAGAAAAAGAAAAACAAACACTAGAAAAAATACTTAAAATACTTGCAAAAAATCAGAATGAAAAAGAATTTTTTCTCAATGAATTTTTGCCTTCACTCGAAGAAGCGAGAAAAATTTGGAAGAGCTCATGA", "aa": "MNSREYPEHKTSTFSYEKFSTYLEKEKQTLEKILKILAKNQNEKEFFLNEFLPSLEEARKIWKSS", "aa_hexdigest": "18a12ce21dd07fc5173e31124346db23", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7906.885199999995, "isoelectric_point": 7.949999427795411 }, "id": "NKOCPLAKPK_416", "locus": "GCA003260355_00379" }, { "type": "cds", "sequence": "contig_1", "start": 200739, "stop": 200915, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCGAAAAACCTTCTCTATGTCTTGGATTACTATCTGCCTCACAAAGGACGAGAGGAGACGGTTTTTGAGCAGATCATCGGTCGTTCTTTGCAGAAGGACTATGAAGTTATCGTGCTGACCTCGCATTTATGTTTACAAATCCAAGATAAACAGCAGATATATAAACTACTCTAA", "aa": "MAKNLLYVLDYYLPHKGREETVFEQIIGRSLQKDYEVIVLTSHLCLQIQDKQQIYKLL", "aa_hexdigest": "83a2f73d4bfff91e7a7c5479c7b22539", "start_type": "ATG", "rbs_motif": "TTATT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6940.0476, "isoelectric_point": 6.704920768737793 }, "id": "NKOCPLAKPK_417", "locus": "GCA003260355_00380" }, { "type": "cds", "sequence": "contig_1", "start": 202130, "stop": 202366, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EFJ1", "UniRef:UniRef90_A0A328EFJ1" ], "nt": "ATGATGTATATTGGTTTGAAGCAAGAAATAAAGAAAATAAAAAAGAAAAATAATAAAGATTTTTGCTGTAGAGAAAAAAATAATATAAATAAAACAACTTCAACTTCTCTGCTCAATGGCTTTCCTATAGAAAAAAAAAGTTATGACTTTGCTAATGCAAGAACAATTTCTGAAGCCACGAATATTCAAAATTCTGAAAGATTTATTAAGAATAAGAACAATACTAATTCTTTTTGA", "aa": "MMYIGLKQEIKKIKKKNNKDFCCREKNNINKTTSTSLLNGFPIEKKSYDFANARTISEATNIQNSERFIKNKNNTNSF", "aa_hexdigest": "2648453029d2d765fb48260d2215a6c3", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A328EFJ1", "query_cov": 1.0, "subject_cov": 0.9069767441860465, "identity": 1.0, "score": 153.0, "evalue": 4.19e-47, "valid": true, "uniref50_id": "UniRef50_A0A328EFJ1" }, "pscc": { "uniref50_id": "UniRef50_A0A328EFJ1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EFJ1" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9097.314799999993, "isoelectric_point": 9.851172065734861 }, "id": "NKOCPLAKPK_418", "locus": "GCA003260355_00381" }, { "type": "cds", "sequence": "contig_1", "start": 202685, "stop": 202825, "strand": "-", "frame": 3, "gene": null, "product": "Response regulatory domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EHV0", "UniRef:UniRef90_A0A328EHV0" ], "nt": "ATGAAAATTTGTTGGATTTTAGATGATGAATATGGTGAAAAAATTTATAAAGGAATCAGACATCTTTTCGCTAATTGGAATTATCCAGTAAAAAAGAATATCGTAAATCCGCTTGAGTTCGTAGACAAAATTCAGGATTGA", "aa": "MKICWILDDEYGEKIYKGIRHLFANWNYPVKKNIVNPLEFVDKIQD", "aa_hexdigest": "83748abb52f6af92a9f0ad50362c11fe", "start_type": "ATG", "rbs_motif": "TTATT", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 202601, "stop": 202825, "observations": { "mutation": [ 202615, 202645, 202654, 202687 ], "stop": [ 202615, 202645, 202654, 202687 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EHV0", "query_cov": 1.0, "subject_cov": 0.6216216216216216, "identity": 1.0, "score": 105.0, "evalue": 9.04e-29, "gene_start": 1, "gene_end": 46, "reference_start": 1, "reference_end": 46, "reference_sequence": "MKICWILDDEYGEKIYKGIRHLFANWNYPVKKNIVNPLEFVDKIQDGNIILLDNYFLGNGGEEALRNDFLGELL" }, "paralog": false, "identity": 0.9459459459459459, "score": 144.0, "evalue": 9.78e-47, "description": "Internal stop codon at 202615, 202645, 202654, 202687. Nonsense mutation around 202615, 202645, 202654, 202687.", "psc": { "uniref90_id": "UniRef90_A0A328EHV0", "product": "Response regulatory domain-containing protein", "uniref50_id": "UniRef50_A0A328EHV0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EHV0", "UniRef:UniRef50_A0A328EHV0" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EHV0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EHV0" ], "product": "Response regulatory domain-containing protein" } }, "id": "NKOCPLAKPK_419", "locus": "GCA003260355_00382" }, { "type": "cds", "sequence": "contig_1", "start": 203120, "stop": 203671, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13361.12", "PFAM:PF13538.12" ], "nt": "GTGGTAAGTTCGGGAGAGGAGCTTTTGAGGCGGTTTTTGGAAGAAGTGACCTTGATGGTAGATACCATAGAAAATAAAGATGAGGATGCTGATGCTGTAAAACTCATGACGGTCCACTCTAGTAAAGGACTGGAGTTTCCGGTGGTCTTTATTGTTGGGGCCGAGGATTCGGTTTTCCCGCTAGCAAATGCAATGCTTGAGCCGAAACTTTTGGAAGAAGAAAGAAGACTAATGTATGTGGCGATTACCAGGGCGAAGGATGTGTTGTTTATTTCTCATGCTCAGAGCAGGATGACTTGGGGACAAACAAAAATGAATCCTGAATCCAGATTTCTTTCAGAATTACCTTCAGAACTAATCAAACATTACGACCTCACTCAAGGAAGCAGTAGAGCTCAGCAATCAAGCATCACTGAGGGAGCTACAGTAAGGCACAAGCTCTTTGGAACAGGCTATGTTTTAGAGATTCGGAATAATATGGGAATCGTAAAATTTCATAATCCTAAATTCGGACTCAGAAAGGTAGAGCTGAGATTTTTGGAGGAATGCTAG", "aa": "MVSSGEELLRRFLEEVTLMVDTIENKDEDADAVKLMTVHSSKGLEFPVVFIVGAEDSVFPLANAMLEPKLLEEERRLMYVAITRAKDVLFISHAQSRMTWGQTKMNPESRFLSELPSELIKHYDLTQGSSRAQQSSITEGATVRHKLFGTGYVLEIRNNMGIVKFHNPKFGLRKVELRFLEEC", "aa_hexdigest": "17bf92192961fe444264fbdb7ccdb346", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13361.12", "name": "UvrD_C", "length": 89, "aa_cov": 0.48633879781420764, "hmm_cov": 0.9887640449438202, "evalue": 1.6358655859782192e-29, "score": 99.81239318847656, "start": 7, "stop": 95 }, { "id": "PF13538.12", "name": "UvrD_C_2", "length": 57, "aa_cov": 0.3114754098360656, "hmm_cov": 0.8421052631578947, "evalue": 1.0909472011075756e-12, "score": 44.123748779296875, "start": 35, "stop": 91 } ], "seq_stats": { "molecular_weight": 20863.80710000001, "isoelectric_point": 5.786800575256348 }, "id": "NKOCPLAKPK_420", "locus": "GCA003260355_00383" }, { "type": "cds", "sequence": "contig_1", "start": 203693, "stop": 203914, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACCCAGCTTTTGAGGGGATTAAAAACTTTATTCAGACTTTTGAAGCTTTAACAACAGCACTAAAAACGCTTAATCCAGCGGATTTTATTGAGCTTATCCTCAAAAAGACTGATTATAAAAACTATTTGATCAAGGAAGAAGGAAATGACCAAGCGGCTGAGGAAAAATATGAGAATGTTGGTCAGCTGATCAATATGGCGAGCAAATATGAATTTTGA", "aa": "MNPAFEGIKNFIQTFEALTTALKTLNPADFIELILKKTDYKNYLIKEEGNDQAAEEKYENVGQLINMASKYEF", "aa_hexdigest": "97dbfdbc3b8dc7bb55a4c37c1fa85252", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8410.447699999999, "isoelectric_point": 4.674973106384278 }, "id": "NKOCPLAKPK_421", "locus": "GCA003260355_00384" }, { "type": "cds", "sequence": "contig_1", "start": 204041, "stop": 204796, "strand": "-", "frame": 3, "gene": null, "product": "DNA 3'-5' helicase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A9E5GRN5" ], "nt": "ATGGGAAAACTCTATCAGGAATATGAAAAAGAGCTTGAGAAAGCAAATTCTCTTGATTTTGATGATCTGCTTTTGTTGCCGTATTTGCTTTTTAAGCAGGATGAGAAGATTTTGAAAAAGTGGCAGAATGCCTTTGATTATATTCTCGTGGATGAGGCACAAGATACGAACTGGATTCAGTTTGAACTGATGAAACTCATGAGCGGAACGGGAGCGAATATTACGCTCATTGGAGATGATTTTCAGAGTATTTATGGCTGGAGAGGAGCCTTGATGGAAAACTTTCTCAATGTAAAACAGTACCGACCTGATATTCAGATGTTTAAGCTACAAACCAATTACCGCTCCAAACCTCATATCGTGAATGCTGGAAATGCTGTGATCAAGCAGAATAAAAACCAGTATGAGAAAAATATTGTTGCCCACAGAGAAGGGAATGACAAAATCACAATTTTCAATCACAATACTGATATTGATGAGGCAGCAAATACCGTTGAACTCATCAAGCAGATGAAAGCCAAAGAAAAACTGAAGTCACGAGGTCAGGTTGCGATTTTATACAGGACGAATGCTCAATCTGGGAACTTTGAAACGATGTTTATTCAGGAGGGGATTCCTTACAAGATTTTTGGAGCTTTCAAGTTTTTTGAGAGGAAGGAAATCAAGGATATTCTTGCGTATTTGAAAGTGATCAATAACCCTTTTGATACCGTATCTTTGAAGAGAGTTCTGAATATTCCTAATAGAAAAATCTGA", "aa": "MGKLYQEYEKELEKANSLDFDDLLLLPYLLFKQDEKILKKWQNAFDYILVDEAQDTNWIQFELMKLMSGTGANITLIGDDFQSIYGWRGALMENFLNVKQYRPDIQMFKLQTNYRSKPHIVNAGNAVIKQNKNQYEKNIVAHREGNDKITIFNHNTDIDEAANTVELIKQMKAKEKLKSRGQVAILYRTNAQSGNFETMFIQEGIPYKIFGAFKFFERKEIKDILAYLKVINNPFDTVSLKRVLNIPNRKI", "aa_hexdigest": "638957945f128877e6eb31d4f77573f2", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A9E5GRN5", "query_cov": 0.8326693227091634, "subject_cov": 0.920704845814978, "identity": 0.7509999999999999, "score": 317.0, "evalue": 5.52e-107, "valid": false, "product": "DNA 3'-5' helicase", "uniref50_id": "UniRef50_A0A9E5GRN5" }, "pscc": { "uniref50_id": "UniRef50_A0A9E5GRN5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A9E5GRN5" ], "product": "DNA 3'-5' helicase" }, "genes": [], "id": "NKOCPLAKPK_422", "locus": "GCA003260355_00385" }, { "type": "cds", "sequence": "contig_1", "start": 204968, "stop": 205312, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00580.27" ], "nt": "GTGCTAACCTACAAAATCGCTTATCTTATCCGAGGATTGCAGCTTCCTGTGCAGAGGATTCTTGCCGTAACCTTCACAAATAAGGCCGCTAATGAAATGAAGGAAAGATTGATTAAGCTGGGAGATGAGATCTTAAGCCAGAATTTTGATCAAGGAAAACAACAAGAAAAAGCTGACTCTGATGCTCTTTCAGATTTTCTTTCTGAAATGCAAAGTTCTGCCAAAATCCAAAAAGGTTTAGAAGCGAGAGAACTCAAATGGATTGGGACTTTTCACTCTATTTTTCTCAAAATTCTCAAAGAAGATATTGAAGCTCTGGGGAGAAAATTTACTAAAAATTTCTGA", "aa": "MLTYKIAYLIRGLQLPVQRILAVTFTNKAANEMKERLIKLGDEILSQNFDQGKQQEKADSDALSDFLSEMQSSAKIQKGLEARELKWIGTFHSIFLKILKEDIEALGRKFTKNF", "aa_hexdigest": "4fb9d92e027350039ede5004a416a9c9", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00580.27", "name": "UvrD-helicase", "length": 113, "aa_cov": 0.956140350877193, "hmm_cov": 0.6283185840707964, "evalue": 1.4482534443517987e-18, "score": 63.92746353149414, "start": 2, "stop": 110 } ], "seq_stats": { "molecular_weight": 13094.064999999986, "isoelectric_point": 9.18701877593994 }, "id": "NKOCPLAKPK_423", "locus": "GCA003260355_00386" }, { "type": "cds", "sequence": "contig_1", "start": 205322, "stop": 205426, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACCTCAAAGACTATATCGCATCAAATCTCAATGAAGAACAGACTTTAGCAGCACTTCATACCCAAACTTCTTCACTCATTATCGCAGGAGCAGGAAGTTGA", "aa": "MNLKDYIASNLNEEQTLAALHTQTSSLIIAGAGS", "aa_hexdigest": "2e8e9b91ce17b51582fac67e7b83e281", "start_type": "ATG", "rbs_motif": "TTCTT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3575.9515, "isoelectric_point": 4.649395561218263 }, "id": "NKOCPLAKPK_424", "locus": "GCA003260355_00387" }, { "type": "cds", "sequence": "contig_1", "start": 205890, "stop": 205985, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGGAAAATGACGAAGGAACAACTTACAGAAACACAAAAGGCAGATCTTATGAAAACTATGACTCCACGTCTTGTGATGTGTATGGTTCTTTGA", "aa": "MRKMTKEQLTETQKADLMKTMTPRLVMCMVL", "aa_hexdigest": "c92bf6ab6760e0ac7ccd6e7d25d62689", "start_type": "ATG", "rbs_motif": "TTATT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3687.5735999999997, "isoelectric_point": 9.624630165100097 }, "id": "NKOCPLAKPK_425", "locus": "GCA003260355_00388" }, { "type": "cds", "sequence": "contig_1", "start": 205998, "stop": 206114, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EJ69", "UniRef:UniRef90_A0A328EJ69" ], "nt": "ATGGTAATGGTGTTTTTGCAAAAGATGGAGACTTCTCTTTGGGTTTCTCTGGGAATTTCTCTTGTGTATGTGATTTTGATTGTGGTGATTACTTTCCCTTTGACTAAACGTAGATAG", "aa": "MVMVFLQKMETSLWVSLGISLVYVILIVVITFPLTKRR", "aa_hexdigest": "d3ee03e8bc3a2aa101a01946ad79f524", "start_type": "ATG", "rbs_motif": "TTCTT", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 205899, "stop": 206114, "observations": { "mutation": [ 205983 ], "stop": [ 205983 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EJ69", "query_cov": 1.0, "subject_cov": 0.5352112676056338, "identity": 1.0, "score": 71.2, "evalue": 1.57e-15, "gene_start": 1, "gene_end": 38, "reference_start": 34, "reference_end": 71, "reference_sequence": "MTKEQLTEAQKADLMKTMTPRLVMCMVLGPLYFMVMVFLQKMETSLWVSLGISLVYVILIVVITFPLTKRR" }, "paralog": false, "identity": 0.971830985915493, "score": 134.0, "evalue": 4.16e-43, "description": "Internal stop codon at 205983. Nonsense mutation around 205983.", "psc": { "uniref90_id": "UniRef90_A0A328EJ69", "uniref50_id": "UniRef50_A0A328EJ69", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EJ69", "UniRef:UniRef50_A0A328EJ69" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EJ69", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EJ69" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 4425.4963, "isoelectric_point": 10.277372550964351 }, "id": "NKOCPLAKPK_426", "locus": "GCA003260355_00389" }, { "type": "cds", "sequence": "contig_1", "start": 206210, "stop": 206500, "strand": "+", "frame": 2, "gene": null, "product": "Magnesium transporter", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MPR6", "UniRef:UniRef90_A0A660MPR6" ], "nt": "ATGAAAAAGGTCTTTAAGCCAAAGCTTTACAGAATTGTGTTGAATGGATTTTATGGGATTTTTGCTGGAGTCCTTGTTACTGTGATTGTAGGCTATATTTCTAATAATGTGGTTTATGCTTTTATTGGTGGGATTCTTGCTATTATTCTTTATGTTTGGTGGCTCATTATCAATAGGTTTATTACTATTGAAGTAGATTCTACTCATCTGATCGTCAAAAAAGGAAAAGAGGAGCGTTATTTTGAAATTGCTCAATGTGCCTTTCGTGCGAAAATCATCACAAGAAGCTGA", "aa": "MKKVFKPKLYRIVLNGFYGIFAGVLVTVIVGYISNNVVYAFIGGILAIILYVWWLIINRFITIEVDSTHLIVKKGKEERYFEIAQCAFRAKIITRS", "aa_hexdigest": "84624c15dcaab7ad0eb29a1468ab0c5c", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 206210, "stop": 206656, "observations": { "mutation": [ 206498, 206513, 206585 ], "stop": [ 206498, 206513, 206585 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A660MPR6", "query_cov": 1.0, "subject_cov": 0.6486486486486487, "identity": 1.0, "score": 184.0, "evalue": 1.94e-58, "gene_start": 1, "gene_end": 96, "reference_start": 1, "reference_end": 96, "reference_sequence": "MKKVFKPKLYRIVLNGFYGIFAGVLVTVIVGYISNNVVYAFIGGILAIILYVWWLIINRFITIEVDSTHLIVKKGKEERYFEIAQCAFRAKIITRSGDTECGLYVADTEGKEHFIDCELIGIEQFGELLECLGIWDNQPSPLLPSQKN" }, "paralog": false, "identity": 0.9797297297297297, "score": 292.0, "evalue": 3.42e-103, "description": "Internal stop codon at 206498, 206513, 206585. Nonsense mutation around 206498, 206513, 206585.", "psc": { "uniref90_id": "UniRef90_A0A660MPR6", "product": "Magnesium transporter", "uniref50_id": "UniRef50_A0A660MPR6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A660MPR6", "UniRef:UniRef50_A0A660MPR6" ] }, "pscc": { "uniref50_id": "UniRef50_A0A660MPR6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MPR6" ], "product": "Magnesium transporter" } }, "id": "NKOCPLAKPK_427", "locus": "GCA003260355_00390" }, { "type": "cds", "sequence": "contig_1", "start": 206466, "stop": 206672, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCCTTTCGTGCGAAAATCATCACAAGAAGCTGAGATACAGAATGTTGATTGTATGTTGCAGATACGGAAGGTAAAGAACATTTTATTGATTGTGAACTTATTGGAATTGAACAATTTTGAGAACTTCTGGAGTGTCTTGGAATCTGGGATAATCAGCCTTCTCCTTTACTTCCTTCTCAGAAAAATTAGTTTTTTTATTCTTTAA", "aa": "MPFVRKSSQEAEIQNVDCMLQIRKVKNILLIVNLLELNNFENFWSVLESGIISLLLYFLLRKISFFIL", "aa_hexdigest": "11bc94ce72ddd586bcd1c740a1565d3e", "start_type": "GTG", "rbs_motif": "TTATT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8046.555799999992, "isoelectric_point": 7.901132392883301 }, "id": "NKOCPLAKPK_428", "locus": "GCA003260355_00391" }, { "type": "cds", "sequence": "contig_1", "start": 206687, "stop": 207115, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBFCEE1", "UniRef:UniRef100_A0A328EG38", "UniRef:UniRef50_A0A328EG38", "UniRef:UniRef90_A0A328EG38" ], "nt": "ATGTCGACTGGAGCGATTCTCATACTTGTTATTTTTATGGTGGTTTTGCCTGCTACTTTTTACTTTTTGACGAAGCAAGTCTCAAATAAAAAGGTGCAAGCCTTTCTTGAACAACATCCTGATGCTGTAAAAGTTTACCTTCCTGGACTTGGAATGAGCGGAGTGCATGCTGTAAGTGTTGCCCTTGAAAGTGTTGATGGTGAAAAGCCTCTGATTTTTATGGAAGGGATGAAAACGGGATTTTATGTTTTGCCAGGTAAGCATACTTTAGAAGCTACTGCGACAAAAACGAGAGCTGGAGTTCTTCACAAGAGTGTTTCTACAGTTTTTGGTCCTTTTAAGCATGAAGTGCAGGTAGAAGCCTACAAGATTTATCAATTTGGTTTTGATGGTAAGGAAGAAGTTTTTACTTTTTCAGAAAAGAACTAA", "aa": "MSTGAILILVIFMVVLPATFYFLTKQVSNKKVQAFLEQHPDAVKVYLPGLGMSGVHAVSVALESVDGEKPLIFMEGMKTGFYVLPGKHTLEATATKTRAGVLHKSVSTVFGPFKHEVQVEAYKIYQFGFDGKEEVFTFSEKN", "aa_hexdigest": "62858c5ce4264cd917a0f89fa038dc0e", "start_type": "ATG", "rbs_motif": "TTTA", "ups": { "uniparc_id": "UPI000DBFCEE1", "uniref100_id": "UniRef100_A0A328EG38", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBFCEE1", "UniRef:UniRef100_A0A328EG38" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EG38", "uniref90_id": "UniRef90_A0A328EG38" }, "psc": { "uniref90_id": "UniRef90_A0A328EG38", "uniref50_id": "UniRef50_A0A328EG38" }, "pscc": { "uniref50_id": "UniRef50_A0A328EG38", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EG38" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15663.102799999993, "isoelectric_point": 7.947678565979004 }, "id": "NKOCPLAKPK_429", "locus": "GCA003260355_00392" }, { "type": "cds", "sequence": "contig_1", "start": 207405, "stop": 207806, "strand": "+", "frame": 3, "gene": null, "product": "Aminoacyl-tRNA synthetase class II (D/K/N) domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MPK7" ], "nt": "ATGGAAATACTGTATGCAAAGGCTAGGCGTTATCGCTCATGCTTGGATCTCAGATCCACCCAGCAGGCAATCCAATTTGTCAAGAGAAGCTTTGAAAAGCAGCTGGAACACTTTTTATCTCTGGAAAAGATTGAAGTTCCTCTCTTCGTAGCAGCTGAAAGTGGGCTTAATGATTCTCTTACAGGAACAGAAAAGGCAGTAAGCTTTGAAATCCAAGCTCTTCCTCAGCTAAGAGCAGAAGTTGTGCATTCTTTAGCAAAATGGAAAAGAATGGCACTTGATTTAGGCTGGTTGCGTTTCTTCTCCAAAAAGCACATATTTAAGCAGTTCAAAGCTCAATTTGGTGATCAAGCTTGGAGCAAGAACTTTATCAAGCAGGAATTCAGCTCCTTTAGCTCTTGA", "aa": "MEILYAKARRYRSCLDLRSTQQAIQFVKRSFEKQLEHFLSLEKIEVPLFVAAESGLNDSLTGTEKAVSFEIQALPQLRAEVVHSLAKWKRMALDLGWLRFFSKKHIFKQFKAQFGDQAWSKNFIKQEFSSFSS", "aa_hexdigest": "4710efbebad13d7679c21414c9502c0f", "start_type": "ATG", "rbs_motif": "TTCTT", "psc": { "uniref90_id": "UniRef90_A0A660MPK7", "query_cov": 1.0, "subject_cov": 0.83125, "identity": 0.887, "score": 230.0, "evalue": 1.38e-75, "valid": false, "product": "Aminoacyl-tRNA synthetase class II (D/K/N) domain-containing protein", "uniref50_id": "UniRef50_A0A660MPK7" }, "pscc": { "uniref50_id": "UniRef50_A0A660MPK7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MPK7" ], "product": "Aminoacyl-tRNA synthetase class II (D/K/N) domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_430", "locus": "GCA003260355_00393" }, { "type": "cds", "sequence": "contig_1", "start": 207869, "stop": 208120, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01336.31" ], "nt": "ATGTCTACAACTAGTATAAAAACTCTATATCAAAGCTATGAAAGCTTGTTTGGAAGTCAACTTATTGTAAAGGGGCGAATTAGAAGTCTTAGAGATTCCAAAACCTTTGGATTTATTGAGCTTAATGATGGTTCTTACCTAAAGAATTTGCAAGTTGTTTTTGAATCAGATTTACAAAACTTTGATGAAATCTGTAAATTTACGACGGGAAGCAGTATCGAAGTGCAAGGAGAACTTGTAAAATCTGAGTGA", "aa": "MSTTSIKTLYQSYESLFGSQLIVKGRIRSLRDSKTFGFIELNDGSYLKNLQVVFESDLQNFDEICKFTTGSSIEVQGELVKSE", "aa_hexdigest": "d70d86edd0c2891f11e92b965614087b", "start_type": "ATG", "rbs_motif": "TTCTT", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01336.31", "name": "tRNA_anti-codon", "length": 61, "aa_cov": 0.7228915662650602, "hmm_cov": 0.9508196721311475, "evalue": 2.8725854330714404e-10, "score": 36.45968246459961, "start": 23, "stop": 82 } ], "seq_stats": { "molecular_weight": 9396.486699999998, "isoelectric_point": 5.0739259719848615 }, "id": "NKOCPLAKPK_431", "locus": "GCA003260355_00394" }, { "type": "cds", "sequence": "contig_1", "start": 208238, "stop": 208438, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00152.25" ], "nt": "TTGAGACCAAGAACTAATACTTTTTCTGCGGTGTTTAGAATAAGGAGTCTCCTTGCCTTTGCTATTCATGATTTCTTCAATAAAAATGGATTTATCTGGGCTCATACACCACTCATTACCGCAAGTGATTGTGAAGGCGCAGGAGAAATGTTTCAAGTGACCACTCTTGATCTCACAAATCCTCCAAAAAAAGAAGACTGA", "aa": "MRPRTNTFSAVFRIRSLLAFAIHDFFNKNGFIWAHTPLITASDCEGAGEMFQVTTLDLTNPPKKED", "aa_hexdigest": "ed1e36c649bcdae91a2d4d65fe056c1b", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00152.25", "name": "tRNA-synt_2", "length": 52, "aa_cov": 0.7878787878787878, "hmm_cov": 1.0, "evalue": 1.2674048834812214e-08, "score": 30.73845672607422, "start": 4, "stop": 55 } ], "seq_stats": { "molecular_weight": 7487.467899999999, "isoelectric_point": 6.710263633728027 }, "id": "NKOCPLAKPK_432", "locus": "GCA003260355_00395" }, { "type": "cds", "sequence": "contig_1", "start": 208604, "stop": 208744, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTGAACCTGAAATTGCTTTCGCTGATCTTTCAGACAATATGCAACTTGCCGAAGATTTGCTCAAGTATGCTTTCCGTTATGTGCTAGAAAATGCTGAAGTAGAGATGGATTTCTTTGATAAATTTATCATGCCATGA", "aa": "MIEPEIAFADLSDNMQLAEDLLKYAFRYVLENAEVEMDFFDKFIMP", "aa_hexdigest": "096a495058ea6ff980fccec9979aa822", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5464.220399999999, "isoelectric_point": 4.0500284194946286 }, "id": "NKOCPLAKPK_433", "locus": "GCA003260355_00396" }, { "type": "cds", "sequence": "contig_1", "start": 208817, "stop": 209260, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00152.25" ], "nt": "TTGAAAAAATCTGGGCAAAAATTTGATTACCAGCCAGAATGGGGAATTGACTTGCAAACTGAACATGAGAGGTATTTATCAGAAAAAGTCTTTAATGGTCCGGTTTTTGTTACTGATTATCCAAAAGATATTAAGGCTTTCTATATGAAGCTCAATGATGATGGGAAAACGGTGAGAGCTATGGATTTGCTCGTTCCTGGGGTAGGGGAGCTGATTGGAGGAAGCCAAAGAGAAGATGATTTTGAGAAACTTAAGAAAAGAATGCAGGAGATGCATCTTGACACCGAAACTTATAGCTGGTTTCTTGATCTCAGAAAATATGGAACGGTGCCTCATGCAGGATTTGGAGTGGGATTTGAGAGGCTTGTAATGTATGTGAGTGGAATGGAGAATATCCGTGATGTGATCCCTTTCCCAAGAGCTCCAAAGACCTGTGAGTTTTAA", "aa": "MKKSGQKFDYQPEWGIDLQTEHERYLSEKVFNGPVFVTDYPKDIKAFYMKLNDDGKTVRAMDLLVPGVGELIGGSQREDDFEKLKKRMQEMHLDTETYSWFLDLRKYGTVPHAGFGVGFERLVMYVSGMENIRDVIPFPRAPKTCEF", "aa_hexdigest": "aae95950e34294b1a68a501bd5d147a5", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0026EF0775", "query_cov": 1.0, "subject_cov": 0.9483870967741935, "identity": 0.68, "score": 220.0, "evalue": 1.32e-71, "valid": false, "product": "amino acid--tRNA ligase-related protein", "uniref50_id": "UniRef50_A0A2D9ECD4" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00152.25", "name": "tRNA-synt_2", "length": 147, "aa_cov": 0.8979591836734694, "hmm_cov": 0.9931972789115646, "evalue": 1.690666157034529e-37, "score": 125.57330322265625, "start": 9, "stop": 140 } ], "seq_stats": { "molecular_weight": 17113.3909, "isoelectric_point": 5.65368366241455 }, "id": "NKOCPLAKPK_434", "locus": "GCA003260355_00397" }, { "type": "cds", "sequence": "contig_1", "start": 209598, "stop": 209822, "strand": "+", "frame": 3, "gene": null, "product": "HIT domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1G2F7D7" ], "nt": "ATGTCAAACTGTATCTTCTGCGACATTATCGCTAAAAAATTACCAGCTTATCTAGTAGCTGAAAATGAATATGCAATTGCCTTTCTTCCCAAAAAAATGGAAAGTTTCGCACACACTTTGATCGTTCCAAAAAAGCACTACGAAAATCTTTTTGATATTCCAGCAAACGAATTAAAAAATCTGATTTCCTTTTTGCAAAATACTGTGAAATCCTATAAAATTTGA", "aa": "MSNCIFCDIIAKKLPAYLVAENEYAIAFLPKKMESFAHTLIVPKKHYENLFDIPANELKNLISFLQNTVKSYKI", "aa_hexdigest": "7c77c901af8706a2dcf16a2b657df4d2", "start_type": "ATG", "rbs_motif": "TTTA", "psc": { "uniref90_id": "UniRef90_A0A1G2F7D7", "query_cov": 0.8378378378378378, "subject_cov": 0.8157894736842105, "identity": 0.565, "score": 78.6, "evalue": 1.03e-17, "valid": false, "product": "HIT domain-containing protein", "uniref50_id": "UniRef50_A0A1G2F7D7" }, "pscc": { "uniref50_id": "UniRef50_A0A1G2F7D7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1G2F7D7" ], "product": "HIT domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_435", "locus": "GCA003260355_00398" }, { "type": "cds", "sequence": "contig_1", "start": 210570, "stop": 210788, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGATAATTTGAAAAAGCAAATAAAAACACAGAATACATTTGTTATGGTTGCTAGTGATCCTACTGCTTATGAAAAAAATGATCAATTTTTACAGCTTGATAGGCAAGCCCTGACTCTTTCAGACTTACATTTTCAGAAATATCTTGTCTTGGATAATCGTAATAAAGATAAGGTGAAAACTGTTTTGGATCAGGCTTCTTTAGTTATCCTTGCCTGA", "aa": "MDNLKKQIKTQNTFVMVASDPTAYEKNDQFLQLDRQALTLSDLHFQKYLVLDNRNKDKVKTVLDQASLVILA", "aa_hexdigest": "bfe4806ad0929dbdd8dc29014d01fbc2", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8325.485699999997, "isoelectric_point": 8.005377769470215 }, "id": "NKOCPLAKPK_436", "locus": "GCA003260355_00399" }, { "type": "cds", "sequence": "contig_1", "start": 210831, "stop": 211163, "strand": "+", "frame": 3, "gene": null, "product": "Dipeptidase E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MMY7", "UniRef:UniRef90_A0A660MMY7" ], "nt": "TTGGGAAAGCATTTGAAATCTATTGACTGTCCGATTGTAGGAATTAGTGCAGGAGCTATGAATTGTGGAGAAATCGTAATCAATAGCTCAGAAAAAAGTAAAAATCCTGATTTGCCTTTGATTTTGAAGGGAATGGGGATTTCACAATATACTATAGTTCCACATTTTGAGAAAAAGCAAAAAAATCCTGATGAAATGAAACTTATCATTCAAGCCTCCCAAAAGATGAAAATTTATGCAGTTCAAGATGGTAGTTATCTCTTGAATGATACAATCTATGGAAGATGTGATCTCATATATCAAGGGAAAATTACCAAGATTTGTGATATTTGA", "aa": "MGKHLKSIDCPIVGISAGAMNCGEIVINSSEKSKNPDLPLILKGMGISQYTIVPHFEKKQKNPDEMKLIIQASQKMKIYAVQDGSYLLNDTIYGRCDLIYQGKITKICDI", "aa_hexdigest": "72b5ad98d638204dd4e4a33d711af12f", "start_type": "TTG", "rbs_motif": "TTTTTA", "genes": [], "truncated": "both", "pseudogene": { "start": 210531, "stop": 211187, "observations": { "mutation": [ 210786, 211161 ], "stop": [ 210786, 211161 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A660MMY7", "query_cov": 1.0, "subject_cov": 0.4330708661417323, "identity": 0.927, "score": 213.0, "evalue": 7.94e-68, "gene_start": 1, "gene_end": 110, "reference_start": 137, "reference_end": 246, "reference_sequence": "MTIIGLSVQFCRAIKEKNHQPLFLTSKICEKQELDGKLSLSKLSNENNFLDTLKKQIKTQNTFVMLASDPTAYEKNDQFLRLDRQALTLSGLHFQEYLVLDNRNKDKVKTVLDQASLVILAGGDTHQQNQFFSAIDLGEHLKPIDCPIVGISAGAMNCGEIVINSSEKSKNPELPLILKGMGISQYLIVPHFEKKQKNPDEMKFIIQVSQKMKIYALQDGSYLLNDTIYGRCDLIYQGKITKICDIGESFLLKK" }, "paralog": false, "identity": 0.908256880733945, "score": 394.0, "evalue": 8.36e-142, "description": "Internal stop codon at 210786, 211161. Nonsense mutation around 210786, 211161.", "psc": { "uniref90_id": "UniRef90_A0A660MMY7", "product": "Dipeptidase E", "uniref50_id": "UniRef50_A0A660MMY7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A660MMY7", "UniRef:UniRef50_A0A660MMY7" ] }, "pscc": { "uniref50_id": "UniRef50_A0A660MMY7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MMY7" ], "product": "Dipeptidase E" } }, "id": "NKOCPLAKPK_437", "locus": "GCA003260355_00400" }, { "type": "cds", "sequence": "contig_1", "start": 211461, "stop": 211595, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACCACTTGAAATTTTTGGTGCCGCTAGTGGGAGCTGTGCTTTTGGCGGGATGTGCAGGTGATCAACCTTCTCAAGTAGCTATTGATCAAAATCCAGCTTCCAAAACTGCGTCTTCTGAGAGTCTCTTCTGA", "aa": "MNHLKFLVPLVGAVLLAGCAGDQPSQVAIDQNPASKTASSESLF", "aa_hexdigest": "6ce3c81889621e15a8fb43d4b063e29f", "start_type": "ATG", "rbs_motif": "TTCTA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4526.151799999999, "isoelectric_point": 5.362327003479003 }, "id": "NKOCPLAKPK_438", "locus": "GCA003260355_00401" }, { "type": "cds", "sequence": "contig_1", "start": 211629, "stop": 211802, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAAAGCCTGCTTTTGATGCTCTGAGCAACAAGGATGAAGTTCAACAAGAAACTTTTAATAAGCCTTGGACAAGGCAGTATCAGTTGACTCAGCAGGATATTGATCAGTTTAAAGAGAATATAAAAGGAGGGTATTGTAAAGATAGCTTTGGGCTTTCTCCTGAGTCTTGA", "aa": "MQKPAFDALSNKDEVQQETFNKPWTRQYQLTQQDIDQFKENIKGGYCKDSFGLSPES", "aa_hexdigest": "6a1505bb15042465ccfd0e2f4dab3dd5", "start_type": "ATG", "rbs_motif": "TTCTT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6659.254800000001, "isoelectric_point": 4.810363578796387 }, "id": "NKOCPLAKPK_439", "locus": "GCA003260355_00402" }, { "type": "cds", "sequence": "contig_1", "start": 211741, "stop": 211899, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAAAGGGAAATAATTATGCACAGAATACTTCCCTGTCACAGGAAATGTTTTCCCTACCTGCAAATCAGAAGGACGAAGCTCTACATCACCATCTCAAGACTCAGGAGAAAGCCCAAAGCTATCTTTACAATACCCTCCTTTTATATTCTCTTTAA", "aa": "MSKGNNYAQNTSLSQEMFSLPANQKDEALHHHLKTQEKAQSYLYNTLLLYSL", "aa_hexdigest": "fbc3ae199504ec8a41462b716db75d9f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6000.641399999999, "isoelectric_point": 6.813199043273925 }, "id": "NKOCPLAKPK_440", "locus": "GCA003260355_00403" }, { "type": "cds", "sequence": "contig_1", "start": 212226, "stop": 212666, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATCCTTTCTTAGCAGCGTATTGGACGATTCCTCATGCTTTGCTTCCAAGTAATGAAGAGTTACTTGAGCTCTTTAGGAAGAGAGAAAGTACTATGCGAGATAATTATGATCATACTTCTGGAAAAGATCCTCATGCAGATTTTGATGATGAGGAACTGAAAGCCTATCTTACTAAGAGATTTGAAAAATTAGTTAAGCAGGTAAAGCCGGAGTATCAAGAACCGCTTAGGAAGGTTTGGACAGAGATTTGGAAGGCAGAACAACCAAAAGCCCCAGATTATCTTATGCTAGCATATAGTCCTGAATTTATTCAAGCAAATGCAATTGAGCAGGACTATACCCAGTTTAGACCTAGGTCTCATTATACTAATTCTTCCTTTCTTAAGTCGTATTTTATGGCGATGAAATGGTTGATGAGAGAAAAGTTTTATTTCTGA", "aa": "MDPFLAAYWTIPHALLPSNEELLELFRKRESTMRDNYDHTSGKDPHADFDDEELKAYLTKRFEKLVKQVKPEYQEPLRKVWTEIWKAEQPKAPDYLMLAYSPEFIQANAIEQDYTQFRPRSHYTNSSFLKSYFMAMKWLMREKFYF", "aa_hexdigest": "efe34f3cfd94b30e98112d6779d7129c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17734.9875, "isoelectric_point": 6.099869728088377 }, "id": "NKOCPLAKPK_441", "locus": "GCA003260355_00404" }, { "type": "cds", "sequence": "contig_1", "start": 212952, "stop": 213761, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAAGAGGATTTGTGTTTTTCGGAGAGAAGTTTACCTTGGATAGCTATATTTTTGATCAGCTAACAGCAGGAAGTGCTGAAAAAGAGTTTTTGCAGAAGCCTAACGTCCAGACGGCAATGATTGTGCCTGATGTATTAGAAAATTATGCTCCTGCCCACGAGCTTGTAAAGCTTCGATTACAAGAAAAATCTCGTGGAGATGATGCTCAGGTGCGTGAAAATGTGAGCTGTGATTTAGGAACTTGTAAACAACTCGGTTCATATGATCAAGTAAAAGCACAGGCTCAAGAAAAAGTGAAAGCAGAACTCAAAGACGAATCGCTCTTGAAAACGGTGTATCATAGTTGGTTAGAGATGCTCGGACAGCTCTTTGTAAAGGTTAAGAATCTTCCTTATTTTAAAGAAAGTCCACTCTACGCCCTCAAATCACTTGCGACTTATATGGGTTCTTATACTGAACTTAAACATGATACCTTGCTTTATGTTAAGCAGGCCTATGCTGAGCTGGGAGGCGGTGGCGATGATCCTTGTTATATTACCGTGTATCCTCCTGAGTTGCCTGTGCCAAAAGGGTATATTGAGTCTGATGTGAATTTCATTGATAAAATGATTACCCTTAATCAGTCAGCACAGCAGTGGTTCAACGATGCAGATAATTTCAAAGGATTTGGTGAATATCTTCAAAAGATTAGAAATCTGAGCCTCAGACAAATGCAGAATCAGAAGATTTCAGATGAGGAATTTGAAGATTTAAGATTATCTTATGATGAATTAGCAAAACTTACGACTCCACGCAAACTTTTCTGA", "aa": "MTRGFVFFGEKFTLDSYIFDQLTAGSAEKEFLQKPNVQTAMIVPDVLENYAPAHELVKLRLQEKSRGDDAQVRENVSCDLGTCKQLGSYDQVKAQAQEKVKAELKDESLLKTVYHSWLEMLGQLFVKVKNLPYFKESPLYALKSLATYMGSYTELKHDTLLYVKQAYAELGGGGDDPCYITVYPPELPVPKGYIESDVNFIDKMITLNQSAQQWFNDADNFKGFGEYLQKIRNLSLRQMQNQKISDEEFEDLRLSYDELAKLTTPRKLF", "aa_hexdigest": "0ebb676e4908dfd1f17a32679c9eba30", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 30869.779400000018, "isoelectric_point": 5.1936857223510735 }, "id": "NKOCPLAKPK_442", "locus": "GCA003260355_00405" }, { "type": "cds", "sequence": "contig_1", "start": 213855, "stop": 214076, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAATGGCTTTGATGGTCAATGATGTAAATGGAGCGAGAATTGTGATGGGACCAGTTTTTGCTCATTATGAGTTTTATAAGTCTGATAATGTCCTAGAGGGAGAACAGAGATATTCTGATAATCAACGACAAGAAGCCTATGACGGTCTTACTTCAGAAATGAAACAGAAATATTACGGAATTGAACAGAGAAAACTCTTACAGGAACTAGCAAAATAG", "aa": "MLMALMVNDVNGARIVMGPVFAHYEFYKSDNVLEGEQRYSDNQRQEAYDGLTSEMKQKYYGIEQRKLLQELAK", "aa_hexdigest": "1626e8610c2e6fb73826fe5bf81f82fa", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8553.6293, "isoelectric_point": 5.264393424987793 }, "id": "NKOCPLAKPK_443", "locus": "GCA003260355_00406" }, { "type": "cds", "sequence": "contig_1", "start": 214141, "stop": 214767, "strand": "+", "frame": 1, "gene": null, "product": "Lipoprotein", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBFC185", "UniRef:UniRef100_A0A660MQP9", "UniRef:UniRef50_A0A660MQP9", "UniRef:UniRef90_A0A660MQP9" ], "nt": "ATGATGAAAAAGGTATTGCCCGTTTTAGTGCTTTGTTTACTTGCAGTAGGGTGCACGAGTTCTACCGAGCTTGGAGCTCATTTTACGCAGTCTAAAGCGTCTGTAAACTCTCCTTCTGAAGGTTATACCGTGAGGGAGGGGGTTATTCGTCGTATTGATCTGATCGACTGGGGAAAGTTTGGTGAAACCGAAGAAGATGATCAGCAGTATACTGCCTGTAATAATGATAAATCTTTGCGAGGGACGGAGAAATGTCCTATCTTTTCATTCCTTACTTTTGTTCCAACTGATGGGAAACTCATAGAAGATGAATGGTATCCTTCAGGTGTTGAGCTTTTTGAAGATGATAGTAAAAAGTCACTTGATCTTGGATGTTTTTCACCAGAAGAGGGAAAAGGCGATAATTATGTCCCAGCATTTATCTATACCAGCAGTTTTGATGAGAAACAGAGTGAAAATGATGATTACACCATCATGGATAAGGCAACAGAAGATGCTCTAATTAAGTTGGTTGATACCGACAAAAAGGTAAAGCTCAAGTTTACTAAGACTCCTTATAATGTTGCTACAGATTTTAAATGGGCAGGATGTGAATCTTTGATAAACAAGGTAGAAATTGTAAAATAG", "aa": "MMKKVLPVLVLCLLAVGCTSSTELGAHFTQSKASVNSPSEGYTVREGVIRRIDLIDWGKFGETEEDDQQYTACNNDKSLRGTEKCPIFSFLTFVPTDGKLIEDEWYPSGVELFEDDSKKSLDLGCFSPEEGKGDNYVPAFIYTSSFDEKQSENDDYTIMDKATEDALIKLVDTDKKVKLKFTKTPYNVATDFKWAGCESLINKVEIVK", "aa_hexdigest": "8bc27d929c447a28ff56b7b90b3b1f42", "start_type": "ATG", "rbs_motif": "TTATT", "ups": { "uniparc_id": "UPI000DBFC185", "uniref100_id": "UniRef100_A0A660MQP9", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBFC185", "UniRef:UniRef100_A0A660MQP9" ] }, "ips": { "uniref100_id": "UniRef100_A0A660MQP9", "uniref90_id": "UniRef90_A0A660MQP9" }, "psc": { "uniref90_id": "UniRef90_A0A660MQP9", "product": "Lipoprotein", "uniref50_id": "UniRef50_A0A660MQP9" }, "pscc": { "uniref50_id": "UniRef50_A0A660MQP9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MQP9" ], "product": "Lipoprotein" }, "genes": [], "id": "NKOCPLAKPK_444", "locus": "GCA003260355_00407" }, { "type": "cds", "sequence": "contig_1", "start": 214784, "stop": 214927, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATACTAGATTAAAAATCAGAATGGATCTCTTGATGCTCTTGATCTTCGAATTGGGAGCATTTTTTTTGATATTTTTCTTGGAATGTCAATATTCTTTTTTTGATTTTGTGGGATTTTTTTCCCTTTTTTGGGAGAATTGA", "aa": "MDTRLKIRMDLLMLLIFELGAFFLIFFLECQYSFFDFVGFFSLFWEN", "aa_hexdigest": "d3dfd8982e9b481ca931d00ffaef202d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5815.881999999998, "isoelectric_point": 4.295857048034668 }, "id": "NKOCPLAKPK_445", "locus": "GCA003260355_00408" }, { "type": "cds", "sequence": "contig_1", "start": 214966, "stop": 215304, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTCGTAAAATAGAACAAATACAGCAAGAAAATATTGCTATTCAGAAGGAATTAGATGCTCTCAAGGCATTGACTGAGCGTGAAGAAAAAGAAAAGAAAAAATCAGAACTTGATCTTCGTATTCAGCATGCAAAGAAAGAAGTTTCGTCTCAGTATCAAGCAGAGACCGACCAAAATATGCGTGAAAAGATAAAAGCAGAGGAAAATAAGCTTGAGAGTTTTTCTACTGAATTAGGAGCATTGGGGGTAGAAGTTCAGGGAGAACAGCCTGCTCATCCGGAAAAAGAAGTTTCGAAAGCAGAAAAAGATCAGAATTCTTCTACTGAAAATCCATGA", "aa": "MARKIEQIQQENIAIQKELDALKALTEREEKEKKKSELDLRIQHAKKEVSSQYQAETDQNMREKIKAEENKLESFSTELGALGVEVQGEQPAHPEKEVSKAEKDQNSSTENP", "aa_hexdigest": "6790f6a8492223d7b1b4257b40918a38", "start_type": "ATG", "rbs_motif": "TTTA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12819.080899999994, "isoelectric_point": 5.094160652160644 }, "id": "NKOCPLAKPK_446", "locus": "GCA003260355_00409" }, { "type": "cds", "sequence": "contig_1", "start": 215317, "stop": 215430, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAAAGATTTTGTCTCAGAGCAATGGACTGATGTTTGGGACGCAGATAAATGGAAAACGGAGACGGGGAAAAATATCCTTCGTGCGGCTGGATTCGTTGCTACGGGAGTCTGA", "aa": "MKDFVSEQWTDVWDADKWKTETGKNILRAAGFVATGV", "aa_hexdigest": "f699c57f6976c1de624404184bea2510", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4201.670500000002, "isoelectric_point": 4.927395057678221 }, "id": "NKOCPLAKPK_447", "locus": "GCA003260355_00410" }, { "type": "cds", "sequence": "contig_1", "start": 215815, "stop": 215955, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTTGTTCGTATGGAGAGCAGATTCCAATTGATATTGGGGCAAGAAAGATTGTAGGGCTGGATGTGCAGTTTTCAAAGCGAGAGGAACTTTTTCATGCTGCTAATATCGTGAATTTCTGTAGGAGGAAGCTTAGATGA", "aa": "MVCSYGEQIPIDIGARKIVGLDVQFSKREELFHAANIVNFCRRKLR", "aa_hexdigest": "89b17f17b2ae22e6e71d25471462e2e5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5322.199, "isoelectric_point": 9.299129295349118 }, "id": "NKOCPLAKPK_448", "locus": "GCA003260355_00411" }, { "type": "cds", "sequence": "contig_1", "start": 216607, "stop": 216957, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATCTTGATTTTCCTCATAATGAAAAGGGGCTAGAAGAGGCGATTATGGTTGCGAATCTCACCAATAAAATTAGAGAAGAATTTAGTAATAAGGCTGCTGAAATGATGCCGTTCTCGTATGGAACTTACAAATTTCCTTTTTCTTTGGATATTGATACTCCGGGCTTTGCTGGGAAACAGGTGCTTTCACATGATGCACTTAAAAAATCATTCCCTACACTTCTCAAAGATTTATCTCAGCAAGGGAAGCTCTCTTCTCAGGAAACGCTTCGAAATCAAGCTAAAGAAGACAAGAAAAAAGGTTGTTCTCAATACATTAAATATCTTCACCAAATGCAGGGAGAGTGA", "aa": "MNLDFPHNEKGLEEAIMVANLTNKIREEFSNKAAEMMPFSYGTYKFPFSLDIDTPGFAGKQVLSHDALKKSFPTLLKDLSQQGKLSSQETLRNQAKEDKKKGCSQYIKYLHQMQGE", "aa_hexdigest": "fa67eeb9b5c9c5abb66366403b919eb9", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13220.92789999999, "isoelectric_point": 7.819579887390138 }, "id": "NKOCPLAKPK_449", "locus": "GCA003260355_00412" }, { "type": "cds", "sequence": "contig_1", "start": 217009, "stop": 217677, "strand": "+", "frame": 1, "gene": null, "product": "Peptidase C39-like domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EJ77", "UniRef:UniRef90_A0A328EJ77" ], "nt": "ATGGCAAATCTCTTGGAATCGATCAAGGATCTTTTAAGCTCTGAAAAGGAAGCAGATACGACGTCCAAAGAGCTTATTCAACTCAAAAAAGAAGTAGCTCAAAAAGTTGTCGATGATGTAGAAAAGCGTCATCCTGATTTATTTGTTTCTGCTTCAAAAGACCTTATTGCAGATTATCTGGTTTCCGATGGTTTTTGGGATTCTGTGATGGATAATCTGGTTTTGGAGAATACCGTTTTGAAAATGACGAATCAAGCCCAGAAGTTTCAGCAGGTTAAAGAACAGATTGATCAGGCTAAGACCAGAGAGGAGCTTAAACAGCTAGAGGATTCTATCCTCAATGGGAAAACAGCTTTAAATTCTGATCTTTCTTCTGTTCAGACTTCGTCTGATGTGTCTTCTGACGTTGCTGCTCCTTCTGATGTTGCTGTTCCTTTACAAGAGGATGTTCCTCCTGTTTCAGCAGTTTCGTCTTCTGCTGTTGTTGCTCCTACTCCTATTGTAAAACCTCCTCAGTCTTCAAGGCAGGGGAAAAAACGTTCTCAAGGAAAAAAGAGAGAAACATCGAGAAGTCAGTCTTCAGTGGATGCTTCTCAATATCATGAAAAAATTCGTGGATTTGATGCTAGTAAAATAGTTTCTTTACCATTTGCTAGGAGTAAAAATTGA", "aa": "MANLLESIKDLLSSEKEADTTSKELIQLKKEVAQKVVDDVEKRHPDLFVSASKDLIADYLVSDGFWDSVMDNLVLENTVLKMTNQAQKFQQVKEQIDQAKTREELKQLEDSILNGKTALNSDLSSVQTSSDVSSDVAAPSDVAVPLQEDVPPVSAVSSSAVVAPTPIVKPPQSSRQGKKRSQGKKRETSRSQSSVDASQYHEKIRGFDASKIVSLPFARSKN", "aa_hexdigest": "47fb776ea9584edd28569a9d5401d616", "start_type": "ATG", "rbs_motif": "TTCTT", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 217009, "stop": 217980, "observations": { "mutation": [ 217675, 217921 ], "stop": [ 217675, 217921 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EJ77", "query_cov": 1.0, "subject_cov": 0.6218487394957983, "identity": 0.973, "score": 289.0, "evalue": 1.17e-94, "gene_start": 1, "gene_end": 222, "reference_start": 1, "reference_end": 222, "reference_sequence": "MANLLESIKDLLSSEKESDTTSKELIQLKKEVAQKVVDDVEKRHPDLFVSASKDLIADYLVSDGFWDSVMDNLVLENTVLKMTNQAQKFQQVKEQIDQAKTREELKQLEDSILNGKTALNSDVSSVQTSTDVSSDVAAPSDVAVPLQEDVPPVSAVSSSAVVAPTPAEKPPHPSRQGKNRSQGKKREPSRSQSSVDASQYHEKIRGFDASKIVSLPFARSKNGVTLCASTARLNAQKFGLVLPSGDAWKASTKNPVAKEYRFSLPSARKHEKPRRGRAPLSLSDFDAMPGVNMADIYTASSSRYGHRSLAFRDISGEWMVLDPYIRVPGTTQTQPKKLKDYMKSVKVIKSHFYAVEA" }, "paralog": false, "identity": 0.9752321981424149, "score": 501.0, "evalue": 1.24e-180, "description": "Internal stop codon at 217675, 217921. Nonsense mutation around 217675, 217921.", "psc": { "uniref90_id": "UniRef90_A0A328EJ77", "product": "Peptidase C39-like domain-containing protein", "uniref50_id": "UniRef50_A0A328EJ77", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EJ77", "UniRef:UniRef50_A0A328EJ77" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EJ77", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EJ77" ], "product": "Peptidase C39-like domain-containing protein" } }, "id": "NKOCPLAKPK_450", "locus": "GCA003260355_00413" }, { "type": "cds", "sequence": "contig_1", "start": 218105, "stop": 218653, "strand": "+", "frame": 2, "gene": null, "product": "Large ribosomal subunit protein bL27", "db_xrefs": [ "KEGG:K02899", "SO:0001217", "UniParc:UPI000DBF7C61", "UniRef:UniRef100_A0A328EJF6", "UniRef:UniRef50_A0A328EJF6", "UniRef:UniRef90_A0A328EJF6" ], "nt": "ATGGGAAAAAAGAGAAGAAGAGTCAGCTTTTATTTTTATTGCTGTTTCTTTTTAATTCCTCTTGAATATCACAATCTAATCTATATACTCGGCGACAGATGTTTTTTTATCTTGTTTGAAAAGCTTATGGCACATAAAAAAGCCGCTGGTTCGGTAAAAAATCTTAAAGATTCTCAGCCAAAATATCGTGGAGTCAAAGTCTTTGGTGGACAACCTGTTCGTGCGGGAAATATTATTATTAGGCAGAAAGGAAGTAAGTATGAATGCGGTAAAAATACTTATACAGCAGCTGATTTTTCTGTTCATGCAGAGGTTGATGGAGTTGTTTCTTTTAGAAAAAAGAATTTTAAGAAGTTTAACGGAAGGACGTATCTTAAGACACTTGTAGAAGTGGTTGATTCAAAATGTGCTTGTGCAGCTTCTTCATCTGCAGCAAACGGTTCAGAAAAGAAGATGAAAGCTCCTTCAGAGAAGAAGGTTGCAAAAACAGCTGTAAAGAAGCCTGCTGTGAAAAAAGCTCCTTCTAAAAAAGCAGAAAAGGCTGAATAA", "aa": "MGKKRRRVSFYFYCCFFLIPLEYHNLIYILGDRCFFILFEKLMAHKKAAGSVKNLKDSQPKYRGVKVFGGQPVRAGNIIIRQKGSKYECGKNTYTAADFSVHAEVDGVVSFRKKNFKKFNGRTYLKTLVEVVDSKCACAASSSAANGSEKKMKAPSEKKVAKTAVKKPAVKKAPSKKAEKAE", "aa_hexdigest": "189592c7a486eaa665e667ee45b946a0", "start_type": "ATG", "rbs_motif": "TTCTT", "ups": { "uniparc_id": "UPI000DBF7C61", "uniref100_id": "UniRef100_A0A328EJF6", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBF7C61", "UniRef:UniRef100_A0A328EJF6" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EJF6", "uniref90_id": "UniRef90_A0A328EJF6" }, "psc": { "uniref90_id": "UniRef90_A0A328EJF6", "product": "Large ribosomal subunit protein bL27", "uniref50_id": "UniRef50_A0A328EJF6", "kegg_orthology_id": "K02899" }, "pscc": { "uniref50_id": "UniRef50_A0A328EJF6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EJF6" ], "product": "Large ribosomal subunit protein bL27" }, "genes": [], "id": "NKOCPLAKPK_451", "locus": "GCA003260355_00414" }, { "type": "cds", "sequence": "contig_1", "start": 218694, "stop": 218927, "strand": "+", "frame": 3, "gene": "rpmF", "product": "50S ribosomal protein L32", "db_xrefs": [ "GO:0003735", "GO:0006412", "GO:0015934", "SO:0001217", "UniParc:UPI000F2DD2D4", "UniRef:UniRef100_A0A660MQJ2", "UniRef:UniRef50_A0A9E2UV71", "UniRef:UniRef90_A0A660MQJ2" ], "nt": "ATGACAATTGGTCCAAAGAAAAAGATCAGTAAAACCCAATCACATAAAAGACATTCAACATGGAAAACAATTAATCTTAAGAGATTGTCTAAGAAATATCAGACAGCAAAATGTCCTAATTGTGGAGCAAATAGGCTTAATCATAGGGTTTGCCCATCTTGCGGTTATTATAATGGAAAGCAGGTGCTTACTATCAAGGCTGCTAAAAAGGATTCAGCGGTTGTAGAAGCTTAG", "aa": "MTIGPKKKISKTQSHKRHSTWKTINLKRLSKKYQTAKCPNCGANRLNHRVCPSCGYYNGKQVLTIKAAKKDSAVVEA", "aa_hexdigest": "ffbd025fcdf4d2f443c94c66299c81d3", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000F2DD2D4", "uniref100_id": "UniRef100_A0A660MQJ2", "db_xrefs": [ "SO:0001217", "UniParc:UPI000F2DD2D4", "UniRef:UniRef100_A0A660MQJ2" ] }, "ips": { "uniref100_id": "UniRef100_A0A660MQJ2", "uniref90_id": "UniRef90_A0A660MQJ2" }, "psc": { "uniref90_id": "UniRef90_A0A660MQJ2", "gene": "rpmF", "product": "50S ribosomal protein L32", "uniref50_id": "UniRef50_A0A9E2UV71", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015934" ] }, "pscc": { "uniref50_id": "UniRef50_A0A9E2UV71", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A9E2UV71" ], "product": "Large ribosomal subunit protein bL32" }, "genes": [ "rpmF" ], "id": "NKOCPLAKPK_452", "locus": "GCA003260355_00415" }, { "type": "cds", "sequence": "contig_1", "start": 218997, "stop": 219218, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATATTAGGAAGAGAATCAATGCTAGAAAAATCGTGCTTTCGTATTTTTATCAGCGTTGTTTTTTTTCTGCTTTAAGCAAAAAACCTGTTGTTGAAGAGTTTCCTAGAGAAAATCGGGCTTTAGAGGACTCTGTTGAGGAAAGAAAGGAGGAAGCTTTTTTGAGTACGGATTTTTTGAATGCTGTTGAACAAAAAAAGCAGGAAGCTGAGCAGTGGTGA", "aa": "MDIRKRINARKIVLSYFYQRCFFSALSKKPVVEEFPRENRALEDSVEERKEEAFLSTDFLNAVEQKKQEAEQW", "aa_hexdigest": "51171e4b0d8f2be8c3d1f5c6d5169ea5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8816.904099999998, "isoelectric_point": 6.30227336883545 }, "id": "NKOCPLAKPK_453", "locus": "GCA003260355_00416" }, { "type": "cds", "sequence": "contig_1", "start": 219501, "stop": 219602, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01029.23" ], "nt": "GTGATTTTGAATGAAATGGTTGAGCTTGCAAAAAGGTACTCTGATGAGGGAGCACCGAAACTCCTTAATGGTGTGCTTCATGCTATTTTTCAGGAAGAATAG", "aa": "MILNEMVELAKRYSDEGAPKLLNGVLHAIFQEE", "aa_hexdigest": "a93c95c1d268ddf8059167e5d5b39c13", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01029.23", "name": "NusB", "length": 28, "aa_cov": 0.8484848484848485, "hmm_cov": 1.0, "evalue": 2.5412281741948405e-08, "score": 30.82620620727539, "start": 2, "stop": 29 } ], "seq_stats": { "molecular_weight": 3759.3097, "isoelectric_point": 4.828438377380371 }, "id": "NKOCPLAKPK_454", "locus": "GCA003260355_00417" }, { "type": "cds", "sequence": "contig_1", "start": 219676, "stop": 219966, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13735.12" ], "nt": "ATGGGAATGTATAACCCTCTTCAGAATGCAAGTGACCTCAGTGATTCTCGATATCTCAAAAAACTTCTTCTAGAGCTAGAACAAGAAGAAGGACAATTTAGAGCCAAAGACCTTGCAATAAAAGGGAAAGATATTATGGAGCATTTCCATCTTAAACCGGGAAAAATTATCGGAGAATTATTAGAAGTAGCTTTAGATCGAGTCCTTGGAGATATAAAAAAGAGAAATAAACCTGATCAGATTTTTGCTAATCTTGCCAATCACTTAAAAAACCTCTCCAACAGAGATTGA", "aa": "MGMYNPLQNASDLSDSRYLKKLLLELEQEEGQFRAKDLAIKGKDIMEHFHLKPGKIIGELLEVALDRVLGDIKKRNKPDQIFANLANHLKNLSNRD", "aa_hexdigest": "b2db928842498656e59e7007f3429d24", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13735.12", "name": "tRNA_NucTran2_2", "length": 35, "aa_cov": 0.3645833333333333, "hmm_cov": 1.0, "evalue": 8.374522950484592e-09, "score": 32.030094146728516, "start": 35, "stop": 69 } ], "seq_stats": { "molecular_weight": 11013.604199999982, "isoelectric_point": 7.987971305847168 }, "id": "NKOCPLAKPK_455", "locus": "GCA003260355_00418" }, { "type": "cds", "sequence": "contig_1", "start": 220138, "stop": 220761, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF12627.12" ], "nt": "TTGGGAATCCAAGATCTCGTGCTAAAGAAACTAAGAACCGTTGGGAATCCTGATACAAGATTTCAAGAAGATGCGCTCAGACTCTTGAGAGGACTGAGATTTGTTAATGTCTTGAATCAAAAGCTCCTCACTACCACCCAAAAGAAAAAATCTGATCCTACCAATAAAGTCAGACTTTTTGATTTTGAACCAGAGACTTGGAAATCAATCAAATCCAATCATACTCTCCTCAAAACTATCGCTAAAGAAAGAATCAAAGAAGAACTCACAAAAGCCTTTTCTCAAGGGAATCCTTTCGGTCTTATTGGACTTTTGGATGAGGCAGAAATGTTACCAATTCTTTTCCCTGCTCTTGCACTGACCAAACACGTTGATCAGCCGATCAGATACCATCCTTTTGATATTTATTCGCATATTATCTTGACACTTTATCATCTTCAGTCAATTAATCCAAATTACTTAGTCAGGTTTGCTATGCTTTATCATGATGTCGGGAAAGTCGGTCAATATGCGGAATATGGGAAAAATCTTTCAAAAGAAGAAATCAGAACTCTCCTCGCAGGTCCACTCAACCATAGATTCAGTTCAGCAGTGCTTGCTGAAGAGGATTTTAGAAAACTTTGA", "aa": "MGIQDLVLKKLRTVGNPDTRFQEDALRLLRGLRFVNVLNQKLLTTTQKKKSDPTNKVRLFDFEPETWKSIKSNHTLLKTIAKERIKEELTKAFSQGNPFGLIGLLDEAEMLPILFPALALTKHVDQPIRYHPFDIYSHIILTLYHLQSINPNYLVRFAMLYHDVGKVGQYAEYGKNLSKEEIRTLLAGPLNHRFSSAVLAEEDFRKL", "aa_hexdigest": "e0eb92941b995b37f01cfafe13234bbf", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF12627.12", "name": "PolyA_pol_RNAbd", "length": 60, "aa_cov": 0.2898550724637681, "hmm_cov": 1.0, "evalue": 1.1604217799901374e-15, "score": 53.556884765625, "start": 60, "stop": 119 } ], "seq_stats": { "molecular_weight": 23887.47060000001, "isoelectric_point": 9.496338081359863 }, "id": "NKOCPLAKPK_456", "locus": "GCA003260355_00419" }, { "type": "cds", "sequence": "contig_1", "start": 220819, "stop": 221442, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01743.26" ], "nt": "ATGCTTCACTTTCATAATCTTAAAAAACTCATTCCTCACCATACCTTTCTAACAAAAAAAATAGGGAAATCAGATTTTTTCCTGGTGGGAGGTTGCGTGAGGGAAATCCTTCTCGGGACAACGAAAGATCCTCTTGATATTGATTTTACAATGGCAGGGAATCCTTTGGAGATCTATCAAAATCTCAATACAGATGGAATTTCTCACTTTATAACCGAGAAATATGGTACCATTACCCTAATTCCCCAAGATAAAAATAATGAACTCAAGTATGAGCTTACCCCTCTTCGTGCTGAGACAGGCTACCAAGATTGTAGACATCCAGGGGAGATTTTTTGGAGTGATGATCTACTTTTAGATGTTCAGAGAAGGGATTTTACCATCAATGCAATCTATTATACTCAGCAGAAGCTGAAAACACCAATATATGAGCAAGAAAAGACAATCAGCGAGGAAGTGTTTCTCAAAATCCTGAATAAGGAGGGCTTTATCTACCTTCAGAATAGTGAAACTCTTATCGTTCAGAATCAGAAACTTCTTTCTCAGCTTATTCCAAATGCAAAGCTTGATACTGATTTTTTATATTATTTGCTAGATATTCAGCCTTATGCCTACATCTTTTGA", "aa": "MLHFHNLKKLIPHHTFLTKKIGKSDFFLVGGCVREILLGTTKDPLDIDFTMAGNPLEIYQNLNTDGISHFITEKYGTITLIPQDKNNELKYELTPLRAETGYQDCRHPGEIFWSDDLLLDVQRRDFTINAIYYTQQKLKTPIYEQEKTISEEVFLKILNKEGFIYLQNSETLIVQNQKLLSQLIPNAKLDTDFLYYLLDIQPYAYIF", "aa_hexdigest": "2de83661931b2cb3c787abf01093d8a0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01743.26", "name": "PolyA_pol", "length": 112, "aa_cov": 0.5362318840579711, "hmm_cov": 0.9464285714285714, "evalue": 1.3998319393501404e-22, "score": 76.84463500976562, "start": 27, "stop": 137 } ], "seq_stats": { "molecular_weight": 24215.519500000017, "isoelectric_point": 5.504992866516112 }, "id": "NKOCPLAKPK_457", "locus": "GCA003260355_00420" }, { "type": "cds", "sequence": "contig_1", "start": 221599, "stop": 221817, "strand": "+", "frame": 1, "gene": null, "product": "23S rRNA (pseudouridine1915-N3)-methyltransferase", "db_xrefs": [ "EC:2.1.1.177", "KEGG:K00783", "SO:0001217", "UniRef:UniRef50_A0A2W4IHD7", "UniRef:UniRef90_A0A328EG64" ], "nt": "ATGTTTGTGATTCTTTCGATATCAGATTCAGATAAGCATTTTCTCGTGCCGATTCAAGAATATGAAAAAAGACTAGGAAAGAGCCTTGTAATTGATAATCTTAAGCCTTTTAAGGATGATAATGTGCAGCTTGTTATTGAGAAGGAAACAGAAAGTCTCTCAAAAACTTTACACAAAAAATATCTTAATTATCACAAAATTCTTCTTATTAAAGAATGA", "aa": "MFVILSISDSDKHFLVPIQEYEKRLGKSLVIDNLKPFKDDNVQLVIEKETESLSKTLHKKYLNYHKILLIKE", "aa_hexdigest": "6ad16c139038a0ab93add0b0e974547e", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 221599, "stop": 222048, "observations": { "mutation": [ 221815, 221818, 221884 ], "stop": [ 221815, 221818, 221884 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EG64", "query_cov": 1.0, "subject_cov": 0.48322147651006714, "identity": 0.986, "score": 141.0, "evalue": 1.19e-41, "gene_start": 1, "gene_end": 72, "reference_start": 1, "reference_end": 72, "reference_sequence": "MFVILSISDSDKHFLVPIQEYEKRLGKSLVIDNLKPFKDDNIQLVIEKETESLSKTLHKKYLNYHKILLIKEGGTYDTLQFSEMIQGENVVFIIGGPYGVDEKRLKSDFSDLIMLALGKMTFPHGLAKLLLIEQIYRATTLWSGKKYHY" }, "paralog": false, "identity": 0.9530201342281879, "score": 283.0, "evalue": 6.56e-100, "description": "Internal stop codon at 221815, 221818, 221884. Nonsense mutation around 221815, 221818, 221884.", "psc": { "uniref90_id": "UniRef90_A0A328EG64", "product": "23S rRNA (pseudouridine1915-N3)-methyltransferase", "ec_ids": [ "2.1.1.177" ], "uniref50_id": "UniRef50_A0A2W4IHD7", "kegg_orthology_id": "K00783", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EG64", "EC:2.1.1.177", "UniRef:UniRef50_A0A2W4IHD7", "KEGG:K00783" ] }, "pscc": { "uniref50_id": "UniRef50_A0A2W4IHD7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4IHD7" ], "product": "Ribosomal RNA large subunit methyltransferase H" } }, "id": "NKOCPLAKPK_458", "locus": "GCA003260355_00421" }, { "type": "cds", "sequence": "contig_1", "start": 221938, "stop": 222048, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTGCTCTCGGAAAAATGACTTTCCCTCATGGTTTAGCTAAGTTGCTCTTGATTGAGCAGATTTATAGGGCAACGACTCTTTGGTCTGGAAAAAAATATCATTATTAA", "aa": "MLALGKMTFPHGLAKLLLIEQIYRATTLWSGKKYHY", "aa_hexdigest": "c3555021adfab4a5ad1eac006446d701", "start_type": "ATG", "rbs_motif": "TTTTT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4194.017500000001, "isoelectric_point": 9.87360706329346 }, "id": "NKOCPLAKPK_459", "locus": "GCA003260355_00422" }, { "type": "cds", "sequence": "contig_1", "start": 222117, "stop": 222686, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCTGGTCAAACGCCATCTCTGCTCCAAGAATCTCTCCATACCGAACAGGTAAAAAGAAAATATAGTAAGCTCGAAAGAGAAATAAAAAGAAAACTTACTGAGTGTTTTCTCTGGGATCATTTCTCAGAAAAAGCTTTTTTGTGGAAAGATATTTCTTCTGCGGGAGAATTTAAAGAAGATTCTGCTTGTAGAGGACTATCTTTTACTAAAGATTTTGATATTAGAGTTCTTGGAAAAGCTGAAAACAGCAAACATATTATGAATCTTGCAAATATCAAGGAAGATACGACTCGAAAGACTAGAAAAATTTTTGAAGCAGAGATTATTGATCTTATTGAAAGAAGTGTAGATGCCGAGGATGAAGCAATAAAAACTAGATATATTAATGGAGTCAAAAACTTTATAGCAAATAATCAGCAAAGAACGATCAATTATCTAAGAGATAATAAGACCTACCTTGCCTTCATTTCTCAATATCATTTTTTGCAAAAAAGTGAGAAAAAAGAAGAGAGAATTAAATTTAATGTCATATCTCAGGTGGGGAAACTGATGAATGAAAAGTTTTGA", "aa": "MSGQTPSLLQESLHTEQVKRKYSKLEREIKRKLTECFLWDHFSEKAFLWKDISSAGEFKEDSACRGLSFTKDFDIRVLGKAENSKHIMNLANIKEDTTRKTRKIFEAEIIDLIERSVDAEDEAIKTRYINGVKNFIANNQQRTINYLRDNKTYLAFISQYHFLQKSEKKEERIKFNVISQVGKLMNEKF", "aa_hexdigest": "894081f1ca996ceec26bfb875c1d1215", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 22310.206000000006, "isoelectric_point": 9.192369651794433 }, "id": "NKOCPLAKPK_460", "locus": "GCA003260355_00423" }, { "type": "cds", "sequence": "contig_1", "start": 222958, "stop": 223095, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAATCAAGGGTCTTTCTTTTTTCAAAATTCTTCTTGCGGAGAAAAATAATTTATATTTTCTAGTTGAAGTTGATTATTCGTTAGAGCCCGCAAAACATCAGCATATTGTGATGTTTTTTTTGCCCATTCCTTAA", "aa": "MQIKGLSFFKILLAEKNNLYFLVEVDYSLEPAKHQHIVMFFLPIP", "aa_hexdigest": "c39ab24bf5f6239f176e755be1fa25a1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5323.3174, "isoelectric_point": 6.702476692199707 }, "id": "NKOCPLAKPK_461", "locus": "GCA003260355_00424" }, { "type": "cds", "sequence": "contig_1", "start": 223290, "stop": 223403, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGGTGTAAATACAGATCATTTTGAGGAGATAAAACAAATTGGGAAACAATGGATTAAGGAATTTGCTACAAGTTTAGAGGCTTATCCAGAAAAATATTTAAAAGATTGA", "aa": "MKGVNTDHFEEIKQIGKQWIKEFATSLEAYPEKYLKD", "aa_hexdigest": "a0fbe0cdb874091b636bbc0c3b6b8fd7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4415.9718, "isoelectric_point": 5.688866996765137 }, "id": "NKOCPLAKPK_462", "locus": "GCA003260355_00425" }, { "type": "cds", "sequence": "contig_1", "start": 223566, "stop": 223706, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAACTTATTATAAAGAAGATTGGAAGGATACTGAAAAATGGAAAACTTACAAAACTTTTTATACCCAATTCTCTGGGAATACCAAAAGAGGTTCTAATGGTAACTATAAAGATTTTAAGTTTAATATCACGGTTTGA", "aa": "MKTYYKEDWKDTEKWKTYKTFYTQFSGNTKRGSNGNYKDFKFNITV", "aa_hexdigest": "3e035710dd82b94342d8c145fada9c53", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5689.282900000002, "isoelectric_point": 9.60180835723877 }, "id": "NKOCPLAKPK_463", "locus": "GCA003260355_00426" }, { "type": "cds", "sequence": "contig_1", "start": 223899, "stop": 224213, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCGGATCGATCTGAAGATAAGGACGAAAAATTTTCAAAAATTGATTTTGGAGAGGGATTAATAATAGATATTTCAGATTTCTATGAAAGAACTCCTGAAAACGATGATCGTATTAATAAAGCTATTGTTGGAATTATTAACTATTTTCTCAAAAAGGGGACTTTTTTCCTCTACTATCAGGGTAATGAAGAAGCTAACAAACAAGACAATCCTGAAAAAAAGATTCACCTTACTAAGGGATTATTAACTACCAATGATCTCAGTCTTGATCCTTCTAATTTTATTAGAGTTTGTTCTGGACTGGAAGTTTGA", "aa": "MSDRSEDKDEKFSKIDFGEGLIIDISDFYERTPENDDRINKAIVGIINYFLKKGTFFLYYQGNEEANKQDNPEKKIHLTKGLLTTNDLSLDPSNFIRVCSGLEV", "aa_hexdigest": "e7928297de3fc9c04dae5855290d1d11", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11971.243799999986, "isoelectric_point": 4.730220603942871 }, "id": "NKOCPLAKPK_464", "locus": "GCA003260355_00427" }, { "type": "cds", "sequence": "contig_1", "start": 224155, "stop": 224325, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATCTCAGTCTTGATCCTTCTAATTTTATTAGAGTTTGTTCTGGACTGGAAGTTTGAAATCAACAGTATTCTTATCTTGGAAGTAAGGCAAATGCTTTTGTCTGAATCTACGATGCTCAGGCTGAGCAAATTCGTTGGGCAAAAATTGGAGAACTTATTGATCGTATGA", "aa": "MISVLILLILLEFVLDWKFEINSILILEVRQMLLSESTMLRLSKFVGQKLENLLIV", "aa_hexdigest": "39ed88b431da3d417a98329bd5f2aea6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6560.007799999998, "isoelectric_point": 5.1057558059692365 }, "id": "NKOCPLAKPK_465", "locus": "GCA003260355_00428" }, { "type": "cds", "sequence": "contig_1", "start": 224435, "stop": 224773, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAGAATTATCTCGGTTTGGGACTCAATGCTTCTTCATTTTTAAATAAAAAAGAGTTTAATTCAGAATTTCTTTCTTCTCTAGGATTCTCTACACCAGAGGCAGGTCTGAGATTCAAAAACACTAATAATCTTGAGGACTACTGCAACAAGCAATTTCTCGATCCCTCAGCATTTGAGCCACTTAGTAAAAAAGATTATTTAATAGAAGAGTTTTTTCTAGGATTAAGAACAGATAAAGGAGTACCTCAGCTTTCAAAGTTCACCTCTCTCCTAATCCCTCACTACGAAAAAAAACTACAAATTTACGAAAACGAAGGATTAATCACCATTGACTGA", "aa": "MENYLGLGLNASSFLNKKEFNSEFLSSLGFSTPEAGLRFKNTNNLEDYCNKQFLDPSAFEPLSKKDYLIEEFFLGLRTDKGVPQLSKFTSLLIPHYEKKLQIYENEGLITID", "aa_hexdigest": "89ccca8964dffeefbcc58faf4dab33d1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12854.410999999987, "isoelectric_point": 5.013335609436035 }, "id": "NKOCPLAKPK_466", "locus": "GCA003260355_00429" }, { "type": "cds", "sequence": "contig_1", "start": 224728, "stop": 224853, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCTGATACCATAGATATGAGCTATCAAATTTTGCTCTTGCAGGAAAATCTAGTATCCACAATAGAACTTATCGAGAAATGGAGAATTATCTCGGTTTGGGACTCAATGCTTCTTCATTTTTAA", "aa": "MPDTIDMSYQILLLQENLVSTIELIEKWRIISVWDSMLLHF", "aa_hexdigest": "d51ac98bee490073bb51117a80ceb9c3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4935.775599999999, "isoelectric_point": 4.290286827087402 }, "id": "NKOCPLAKPK_467", "locus": "GCA003260355_00430" }, { "type": "cds", "sequence": "contig_1", "start": 224846, "stop": 225205, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCTTCAACTCGCAGGAAGACACACACGTTTCCTCGGACTGGTTGATTTTCTCCGCAACCTCCAGCCTCTCAAACAAGATAATACAGTCTTTAATTTTGATTTTATCGCTTTTGGAAAATGGAACCAGAGTAAAAAAGGCAATCCCTACCTGCGGACTCCTTCAGCTTTACAATTTTTTACAGATTTTGTAAATTCACAATTTGCCGATAGTTTCTCGCTTTATACTTTGGAACTTTTTGAAAATCAAGCTCGAAAAAGAAAAAAATCCGACGCACTCATCTCAGGAGCATACTTTGGAACTGATGAACAAATCTATGAGGAGTTTAGCCTTTTAAAGGATATTCTTTTAGATGCCTGA", "aa": "MLQLAGRHTRFLGLVDFLRNLQPLKQDNTVFNFDFIAFGKWNQSKKGNPYLRTPSALQFFTDFVNSQFADSFSLYTLELFENQARKRKKSDALISGAYFGTDEQIYEEFSLLKDILLDA", "aa_hexdigest": "4f031e2dd00bb2244e6ec042c883b97b", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13832.508499999982, "isoelectric_point": 6.564755821228027 }, "id": "NKOCPLAKPK_468", "locus": "GCA003260355_00431" }, { "type": "cds", "sequence": "contig_1", "start": 225425, "stop": 225607, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTTCACTCTACATGCATATTCCTTTTTGCCAATCCAAATGCAACTACTGTGCATTCTCTTCTTTTGCTCTAACAGAAAAATCTGATCAAATCGCACAGTACCTCTCGGCTTTAAAAGAAGAAATCAAACATTACAGTAAATATTTTCATCACGAAGGAATAAAAACTCTTTACTTCTGA", "aa": "MLSLYMHIPFCQSKCNYCAFSSFALTEKSDQIAQYLSALKEEIKHYSKYFHHEGIKTLYF", "aa_hexdigest": "ef7d9c4a04d1bf1f59df63bafb4a9aaf", "start_type": "ATG", "rbs_motif": "TTTA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7134.171799999998, "isoelectric_point": 7.742857551574708 }, "id": "NKOCPLAKPK_469", "locus": "GCA003260355_00432" }, { "type": "cds", "sequence": "contig_1", "start": 225760, "stop": 226179, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGGTAAAATGACAAAGAAAATGTTGGCAGTAGCTTTGATGGCTGGGTCTGTATTGGTATTGGCTGGATGTAATAAAACTCCTGCTGAAACTGAAAATGAAGTTGTATCACCTGATGCAAGCGGTGTTGTAATTGATGTAGATGCAACATCTGGAGCAACTATGGACGATTCTGCAGCTTCTAGTTTTGATGAAACTTCTCTTACTGGAGATGAATCACTAACTTCTGGTGCAGATACTGCTTCTGTTGATGAAGCTGCTAATGCTGCTCTAGCTGCTCAACAAGAAGCTCTTAAGAATGCTGGTGTTGATGCTGCTGCTGTTCAAGCTGCTCAACAAGCTGCTCTTAAGAATGCTGGTGTTGATGCTGCTGCTGTTCAAGCTGCTCAACAAGCTGCTCTTAAGGCTGCTAAATAG", "aa": "MNGKMTKKMLAVALMAGSVLVLAGCNKTPAETENEVVSPDASGVVIDVDATSGATMDDSAASSFDETSLTGDESLTSGADTASVDEAANAALAAQQEALKNAGVDAAAVQAAQQAALKNAGVDAAAVQAAQQAALKAAK", "aa_hexdigest": "6f161f774163385ece1afd60287e9a96", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13567.895399999981, "isoelectric_point": 4.123691749572752 }, "id": "NKOCPLAKPK_470", "locus": "GCA003260355_00433" }, { "type": "cds", "sequence": "contig_1", "start": 226273, "stop": 226764, "strand": "+", "frame": 1, "gene": null, "product": "Pseudouridine synthase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4IIZ8", "UniRef:UniRef90_A0A328EHW9" ], "nt": "ATGCTACTCAGAGCATACTTACAGACAAAACATCAACTCCCAAGGAGAAAAATAACGCTACTCATTGATAATGGGAAAGTTTTTATTAACAAAGAAAAGGTAAATAACTATAAAGCTGAACTGCATGAGAAAGATCTGCTTGAAATTCCAGATATAAAGCTTCAAGAATATATTTTATCGGATACATCTAATACAGAAAATAAAAAACCAGATTTTATCCTTTTCAATAAACCTAAGGGATATACTTGTTCAAAGGCAGATCCACATAATCAGGTTTTTTATGATCTTTTGCCTCAAAAGTTTAGGAAATATTATTATATTGGGAGACTCGATAAGGAAAGTTGTGGATTGATGCTTTTGACTAGTAAGCCGGAACTCGTCCATCAGTTTGAGCATCCAAGCAAGGAGATTGAAAAGAGTTATCTTGTTCAGCTCGATAAGCCTTTTGATCGGAAGCTTAAAAATAGAATCTTAGCTGGAATCTCTGAATGA", "aa": "MLLRAYLQTKHQLPRRKITLLIDNGKVFINKEKVNNYKAELHEKDLLEIPDIKLQEYILSDTSNTENKKPDFILFNKPKGYTCSKADPHNQVFYDLLPQKFRKYYYIGRLDKESCGLMLLTSKPELVHQFEHPSKEIEKSYLVQLDKPFDRKLKNRILAGISE", "aa_hexdigest": "49af16cea87a972bdb06606fcd6bdce4", "start_type": "ATG", "rbs_motif": "TTATT", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 226273, "stop": 226962, "observations": { "mutation": [ 226762, 226807 ], "stop": [ 226762, 226807 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EHW9", "query_cov": 1.0, "subject_cov": 0.7117903930131004, "identity": 1.0, "score": 330.0, "evalue": 1.7e-113, "gene_start": 1, "gene_end": 163, "reference_start": 1, "reference_end": 163, "reference_sequence": "MLLRAYLQTKHQLPRRKITLLIDNGKVFINKEKVNNYKAELHEKDLLEIPDIKLQEYILSDTSNTENKKPDFILFNKPKGYTCSKADPHNQVFYDLLPQKFRKYYYIGRLDKESCGLMLLTSKPELVHQFEHPSKEIEKSYLVQLDKPFDRKLKNRILAGISEGGEFLRTKQLEKSDFGLLEITLNEGKKRHIRRIFKALGYVVTDLQRIRIGPYCLGSLPEGKWKFLN" }, "paralog": false, "identity": 0.9912663755458515, "score": 459.0, "evalue": 6.94e-167, "description": "Internal stop codon at 226762, 226807. Nonsense mutation around 226762, 226807.", "psc": { "uniref90_id": "UniRef90_A0A328EHW9", "product": "Pseudouridine synthase", "uniref50_id": "UniRef50_A0A2W4IIZ8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EHW9", "UniRef:UniRef50_A0A2W4IIZ8" ] }, "pscc": { "uniref50_id": "UniRef50_A0A2W4IIZ8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4IIZ8" ], "product": "Pseudouridine synthase" } }, "id": "NKOCPLAKPK_471", "locus": "GCA003260355_00434" }, { "type": "cds", "sequence": "contig_1", "start": 226813, "stop": 226962, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAAATTACCCTTAATGAAGGGAAGAAAAGGCATATTAGGAGAATTTTTAAAGCTCTTGGCTATGTGGTGACTGATTTGCAAAGGATTCGTATCGGTCCTTATTGTCTTGGTTCTTTGCCTGAGGGGAAATGGAAGTTCCTTAATTAG", "aa": "MEITLNEGKKRHIRRIFKALGYVVTDLQRIRIGPYCLGSLPEGKWKFLN", "aa_hexdigest": "df0f7cb41ef7450ff3c99579d117e266", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5759.796699999998, "isoelectric_point": 10.126065254211426 }, "id": "NKOCPLAKPK_472", "locus": "GCA003260355_00435" }, { "type": "cds", "sequence": "contig_1", "start": 227083, "stop": 227262, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAAAGAATAAAAAAGAAAATGGCTGTCTTGTATTACTCTTTCTCATTGGAGTAATCCTCGTCACAATGCCTATGATACTACTCTTCTGGATATCCGAAACCATATATGGGATGCGAAAGCAGGACGGAGAAATTCTCGAAAGAGATATAGTATTCCAAGATCCAGACAGCAATTAA", "aa": "MEKNKKENGCLVLLFLIGVILVTMPMILLFWISETIYGMRKQDGEILERDIVFQDPDSN", "aa_hexdigest": "a9d5cd4b362b34168641896fda82edaf", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6859.079100000001, "isoelectric_point": 4.606538963317871 }, "id": "NKOCPLAKPK_473", "locus": "GCA003260355_00436" }, { "type": "cds", "sequence": "contig_1", "start": 227417, "stop": 227803, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01168.26" ], "nt": "TTGAAACCTGACCTGAAAACCCTTAATACAATCTCGATTTCTAAGGCTGCGATTTTGCATAATCTGGATATTATGAGAAAACTTCAGCCAAAATCTGCTTTCTTTCCTGTTTTGAAATCGAATGCTTATGGACATGGGATTTTGCAGATATTGGAAATTTTGAAGGGGCAGAATTTTCCGTATTTGGTCGTTGATAGCTTCCCTGAATATCAATTGGTGCATAATCATTCCAAGTTTGATATTTTATTGCTTGGTGAGACTTTGCCTGAAAACTATCGTTTTTTTGATCTGAGGAGAACTGCGTTTGCGGTTTATACTTTGAATGCCTTGAAGGCACTGGCTCAACTGCACAAAAAAGTCAGAATTCATCTTTTTTTTGATACTTGA", "aa": "MKPDLKTLNTISISKAAILHNLDIMRKLQPKSAFFPVLKSNAYGHGILQILEILKGQNFPYLVVDSFPEYQLVHNHSKFDILLLGETLPENYRFFDLRRTAFAVYTLNALKALAQLHKKVRIHLFFDT", "aa_hexdigest": "c46f4f407e8b425701052c14c6323dd6", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01168.26", "name": "Ala_racemase_N", "length": 122, "aa_cov": 0.90625, "hmm_cov": 1.0, "evalue": 1.1928556565433815e-16, "score": 57.54050064086914, "start": 13, "stop": 128 } ], "seq_stats": { "molecular_weight": 14797.214199999977, "isoelectric_point": 9.655188179016115 }, "id": "NKOCPLAKPK_474", "locus": "GCA003260355_00437" }, { "type": "cds", "sequence": "contig_1", "start": 227804, "stop": 228013, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01168.26" ], "nt": "ATGCACAGAGAAGGGGTTCAAGAACAGAATTTACCTCAGATTTTGGCGTTTCTCAAAGAACATAAGCAGCTTGAATTGGAAGGACTGATGTCTCACTTACACAGTGCTGATACTGATGAAGCATCGATTCAAAAGCAAGTTACTTTGTTTAAAAAAATGGGAAATCTGGTTGAACAGGCAGGTTTTGCTCCCAAATGGAAACATATTTGA", "aa": "MHREGVQEQNLPQILAFLKEHKQLELEGLMSHLHSADTDEASIQKQVTLFKKMGNLVEQAGFAPKWKHI", "aa_hexdigest": "2e560a8412392b3b7529b3a5ac838e0f", "start_type": "ATG", "rbs_motif": "TTTTT", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01168.26", "name": "Ala_racemase_N", "length": 70, "aa_cov": 1.0, "hmm_cov": 1.0, "evalue": 2.759558048447081e-14, "score": 49.81033706665039, "start": 1, "stop": 69 } ], "seq_stats": { "molecular_weight": 7934.0543, "isoelectric_point": 6.495184898376464 }, "id": "NKOCPLAKPK_475", "locus": "GCA003260355_00438" }, { "type": "cds", "sequence": "contig_1", "start": 228802, "stop": 229215, "strand": "+", "frame": 1, "gene": null, "product": "Haloacid dehalogenase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EJ84", "UniRef:UniRef90_A0A328EJ84" ], "nt": "TTGGCGGCTTCAGATGATTTGCAGGAGATTTTGGATAAGATGGCCATTCCTAAGGCTTCTCAAAAGGAATTTATAGAAAAAGTGCGTGATGATATTGCGCAAATTCAACCTCATCCTGATGCGATTCCGCTTTTGAAGTATTTAAAAGAGCGCAAGTATTCGTTGGCGATAGATAGCTTCTTAATCCCTCTTTATGCTGGTCCTATCAAGGCGCATTTTTCTGATTATGTTGAAAAGTTTGTTTTTTCTTTTGAACAGCAGCATATGAAAGGAGAGAGTCAGCATTATTCTGATCTTTTACAATCTCTTAATATCCCTGCACATCAGGCTCTCTTTGTAGGAGATCATTATAGAAGGGACTATCTTGCTTCTAAAGCAGCTGGAATGAATGCCTTACACCTTTCTAATAAGTGA", "aa": "MAASDDLQEILDKMAIPKASQKEFIEKVRDDIAQIQPHPDAIPLLKYLKERKYSLAIDSFLIPLYAGPIKAHFSDYVEKFVFSFEQQHMKGESQHYSDLLQSLNIPAHQALFVGDHYRRDYLASKAAGMNALHLSNK", "aa_hexdigest": "87a13ac94fd53dfc881eb67afd69811c", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 228634, "stop": 229278, "observations": { "mutation": [ 228667, 228793, 229213 ], "stop": [ 228667, 228793, 229213 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EJ84", "query_cov": 1.0, "subject_cov": 0.6401869158878505, "identity": 0.993, "score": 271.0, "evalue": 7.09e-91, "gene_start": 1, "gene_end": 137, "reference_start": 57, "reference_end": 193, "reference_sequence": "MNEPREEPESFGKNNYDQLSHIIFDAYGTLLSPSKYNIWQELGKRAKATREEVGRALAASDDLQEILDKMAIPKASQKEFIEKVRDDIAQIQPHPDAIPLLKYLKERKYSLAIDSFLIPLYAGPIKAHFSDYVEKFVFSFEQQHMKGESQHYSDLLQSLNIPAHQALFVGDHYRRDYLASKAAGMNALHLSNKGGKVGKERLTTLDELLTVLNI" }, "paralog": false, "identity": 0.985981308411215, "score": 417.0, "evalue": 5.86e-151, "description": "Internal stop codon at 228667, 228793, 229213. Nonsense mutation around 228667, 228793, 229213.", "psc": { "uniref90_id": "UniRef90_A0A328EJ84", "product": "Haloacid dehalogenase", "uniref50_id": "UniRef50_A0A328EJ84", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EJ84", "UniRef:UniRef50_A0A328EJ84" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EJ84", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EJ84" ], "product": "Haloacid dehalogenase" } }, "id": "NKOCPLAKPK_476", "locus": "GCA003260355_00439" }, { "type": "cds", "sequence": "contig_1", "start": 229576, "stop": 229731, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTAATGGAAAAAATTACGATTGTCTCAGAAATTCAGCCTCCTCAAAATGATAAGGAACGAAAGACGAGAGAAAGAGTTGCCTGCTTTTTAAAGCACCCGAATGAAGAAAAATTCGCTTTGATTGAAGAACAATATGGCATTACTGTCCCTTGA", "aa": "MVMEKITIVSEIQPPQNDKERKTRERVACFLKHPNEEKFALIEEQYGITVP", "aa_hexdigest": "6cd1b7d21fedc6377a9cb6a944617d23", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6014.905199999998, "isoelectric_point": 5.738998985290528 }, "id": "NKOCPLAKPK_477", "locus": "GCA003260355_00440" }, { "type": "cds", "sequence": "contig_1", "start": 229971, "stop": 230096, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAGCATAATCAATGCGATTTTCCTAATATTTCTTATGTTATCAACCGTTTTAGCCGAGATAAAGAGGGTATTGCAAAAGGTATTGAGGGAGAAATAATTCATGCACTCAATAAAGATGAATGA", "aa": "MQHNQCDFPNISYVINRFSRDKEGIAKGIEGEIIHALNKDE", "aa_hexdigest": "184f85373cd118012c774a380f99200c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4731.2426, "isoelectric_point": 5.524602317810058 }, "id": "NKOCPLAKPK_478", "locus": "GCA003260355_00441" }, { "type": "cds", "sequence": "contig_1", "start": 230151, "stop": 230567, "strand": "-", "frame": 2, "gene": null, "product": "Pyrimidine 5'-nucleotidase (UMPH-1)", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1V5ZQ20" ], "nt": "ATGCAAGTCCATATCAAAAATCTAGAACTTTTTGCCAAGAAAAAGCAAGCTATTCAAGCGGCTGGGAAAGAGTCTTGCTTTATCATTGCTGATTTTGATAGAACGCTTACTTACGGGACTTTCAATGGGGAAAAAACACCGAGTATTATCTCACTTTTGAGAGATGGTAATCATTTGACCGACGATTATGCAGAGAAAGCTCATGCACTCTTTGATCATTATCATGCTATAGAGTGCGATTCTAGCTTGCCTCTGGAATATAGAAAAGCTCAGATGTGGGAATGGCGAGAAAAGCACGATGAACTCTTGATTGCCTCAAAACTCAGACTTACAGACCTCAAAGATATTGCGCAAAATGGTCATCTCCAGCTGAGGAAAGGTGTTGGAGAATGTCTTAGAAAATTAGATGAAAACTGA", "aa": "MQVHIKNLELFAKKKQAIQAAGKESCFIIADFDRTLTYGTFNGEKTPSIISLLRDGNHLTDDYAEKAHALFDHYHAIECDSSLPLEYRKAQMWEWREKHDELLIASKLRLTDLKDIAQNGHLQLRKGVGECLRKLDEN", "aa_hexdigest": "5c1f35f625af3ca500db79b8a91deba7", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A1V5ZQ20", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.703, "score": 199.0, "evalue": 8.87e-64, "valid": false, "product": "Pyrimidine 5'-nucleotidase (UMPH-1)", "uniref50_id": "UniRef50_A0A1V5ZQ20" }, "pscc": { "uniref50_id": "UniRef50_A0A1V5ZQ20", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1V5ZQ20" ], "product": "Pyrimidine 5'-nucleotidase (UMPH-1)" }, "genes": [], "id": "NKOCPLAKPK_479", "locus": "GCA003260355_00442" }, { "type": "cds", "sequence": "contig_1", "start": 230673, "stop": 230936, "strand": "-", "frame": 2, "gene": null, "product": "Isoleucine--tRNA ligase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EG55", "UniRef:UniRef90_A0A328EG55" ], "nt": "TTGAGTGCCGAGGATTATGAAGTAGCGTATGAAGGGCTTGATGCCAATAATATCGCAGTGGAGGGGAATATGATCGCAGAGCTTGACTTGACTTTGACTCCAGAGCTTGAGAGAGAAGGGGTAGCAAGAGAGATTTCTCGTTTCCTCAACCAAATGAGAAAAGATGCGGATTTCGCAGTAGAGCAAAAGGTAAAGCTGACCTATACGACTGCAAGTGAATATTTAAAAGTGATTTTGAAAGAATTTGATGAAATATTTAAGTGA", "aa": "MSAEDYEVAYEGLDANNIAVEGNMIAELDLTLTPELEREGVAREISRFLNQMRKDADFAVEQKVKLTYTTASEYLKVILKEFDEIFK", "aa_hexdigest": "347a1f8326a67fcc233a721d8e216834", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 230571, "stop": 231185, "observations": { "mutation": [ 230606, 230633, 230675, 230948, 231113 ], "stop": [ 230606, 230633, 230675, 230948, 231113 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EG55", "query_cov": 1.0, "subject_cov": 0.4264705882352941, "identity": 0.9890000000000001, "score": 164.0, "evalue": 8.9e-50, "gene_start": 1, "gene_end": 87, "reference_start": 84, "reference_end": 170, "reference_sequence": "MLNEQQLLEKYGEIIKEEVNVKEIGPFSSDKPLVKVFKPLGSQLSAKFGKDTGQIIANGKQGNIRELENGQIEVFSPQGGNWVLSAEDYEVAYEGLDANNIAVEGNMIAELDLTLTPELEREGVAREISRFLNQMRKDADFAVEQKVKLTYTTASEYLKVILKEFDEIFKGEALLLTIEEKEADGVISSEFVSGNESIHFSLAE" }, "paralog": false, "identity": 0.9754901960784313, "score": 377.0, "evalue": 4.12e-136, "description": "Internal stop codon at 230606, 230633, 230675, 230948, 231113. Nonsense mutation around 230606, 230633, 230675, 230948, 231113.", "psc": { "uniref90_id": "UniRef90_A0A328EG55", "product": "Isoleucine--tRNA ligase", "uniref50_id": "UniRef50_A0A328EG55", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EG55", "UniRef:UniRef50_A0A328EG55" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EG55", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EG55" ], "product": "Isoleucine--tRNA ligase" } }, "id": "NKOCPLAKPK_480", "locus": "GCA003260355_00443" }, { "type": "cds", "sequence": "contig_1", "start": 230946, "stop": 231086, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAAAGTTTTTAAACCGTTGGGAAGCCAACTTTCTGCAAAATTTGGGAAAGATACCGGGCAAATCATCGCAAATGGAAAACAAGGGAATATCAGAGAGCTTGAAAACGGACAGATTGAAGTTTTCTCTCCACAGGGGTGA", "aa": "MKVFKPLGSQLSAKFGKDTGQIIANGKQGNIRELENGQIEVFSPQG", "aa_hexdigest": "2b7d7c64466bed86d1013335f488b687", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4961.6094, "isoelectric_point": 9.398732948303223 }, "id": "NKOCPLAKPK_481", "locus": "GCA003260355_00444" }, { "type": "cds", "sequence": "contig_1", "start": 231449, "stop": 231676, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGATCAGATTTCTCAGCTTTTAGACTCAATCCTCTTTCAAGAAGAAAAAGAAGTCCTGCTTCCTTTGCCGACCTTTAGTGCAAAAAAACAGAATGGGAAAAGACTCTATAAAGATGCAAGAAAAGGAACTGCAGAAATCCAAGAAAAGCCAATGAAAATCTATGCTTACGAAATTTTAGACTATCAGTTTCCTCTTTTGAAGCTCAGACTTGAAGTTGGCTCTTGA", "aa": "MDQISQLLDSILFQEEKEVLLPLPTFSAKKQNGKRLYKDARKGTAEIQEKPMKIYAYEILDYQFPLLKLRLEVGS", "aa_hexdigest": "5a93e96c69a0df6681227a4015141413", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8760.139199999998, "isoelectric_point": 7.925888252258301 }, "id": "NKOCPLAKPK_482", "locus": "GCA003260355_00445" }, { "type": "cds", "sequence": "contig_1", "start": 231725, "stop": 231904, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAAGTGGACTTATGATCCTCGGAATTGGGAAAGGCACGAAAGAACTAACATCTTTGATTGGGCTTGATAAAAGTTATGAAACGACGATTAATTTTTCGCTTCTGACTGATACCCGAGATGCAAGTTTCTGGGAGAAGGAAGAGAGATTTGAAGTCATTGAGTCTCAGGATACCTGA", "aa": "MASGLMILGIGKGTKELTSLIGLDKSYETTINFSLLTDTRDASFWEKEERFEVIESQDT", "aa_hexdigest": "294e97165209c665c6d2851e08960a55", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6617.377399999999, "isoelectric_point": 4.409250831604004 }, "id": "NKOCPLAKPK_483", "locus": "GCA003260355_00446" }, { "type": "cds", "sequence": "contig_1", "start": 231926, "stop": 232153, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAATTTTAAGAAACTTTTTTACTCCAATAGTTTCAAAGCATAAGAAGCAGAATTTTAAAGAACAAAAGCTGCTCTCCTTGAAAAAAGTCAGCTTTTTTCTATTATTTAACGAAAATTTTATCCTTTTTTTGACTTCAATGCTCCTTGCCTGCTACAAACCAAGCGGAATTTCCAGTTTTGATGTGGTAAAAATCATTAGGACACATTTCCAAAATAAAGTCTGA", "aa": "MQILRNFFTPIVSKHKKQNFKEQKLLSLKKVSFFLLFNENFILFLTSMLLACYKPSGISSFDVVKIIRTHFQNKV", "aa_hexdigest": "430bf2f6161f08f55bd1e0f10ee9c804", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8884.568599999991, "isoelectric_point": 10.246427726745605 }, "id": "NKOCPLAKPK_484", "locus": "GCA003260355_00447" }, { "type": "cds", "sequence": "contig_1", "start": 232205, "stop": 232381, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EG73", "UniRef:UniRef90_A0A328EG73" ], "nt": "ATGGTTTTTATTGCTCTTGGTGGTGTTCAGGTTTTTAAGTCTTTTGATTTGATGACTTGGGCGAAGATAATGGATGTGATCTTTAAAATTCTGCAAAATGATGTTATTGGTCTCTTCCTTATGATGGCGAGTTTTGAGCTATGGGGCAACCTTATTGCTTGGAAAATTGGCTTTTGA", "aa": "MVFIALGGVQVFKSFDLMTWAKIMDVIFKILQNDVIGLFLMMASFELWGNLIAWKIGF", "aa_hexdigest": "d4736f38adf8734fd94fde89430dbdd8", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 232205, "stop": 232429, "observations": { "mutation": [ 232379 ], "stop": [ 232379 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EG73", "query_cov": 1.0, "subject_cov": 0.7837837837837838, "identity": 1.0, "score": 115.0, "evalue": 1.63e-32, "gene_start": 1, "gene_end": 58, "reference_start": 1, "reference_end": 58, "reference_sequence": "MVFIALGGVQVFKSFDLMTWAKIMDVIFKILQNDVIGLFLMMASFELWGNLIAWKIGFGNKQKFKIRFFLAWYS" }, "paralog": false, "identity": 0.9864864864864865, "score": 150.0, "evalue": 5.44e-49, "description": "Internal stop codon at 232379. Nonsense mutation around 232379.", "psc": { "uniref90_id": "UniRef90_A0A328EG73", "uniref50_id": "UniRef50_A0A328EG73", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EG73", "UniRef:UniRef50_A0A328EG73" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EG73", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EG73" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 6659.058599999997, "isoelectric_point": 5.91951961517334 }, "id": "NKOCPLAKPK_485", "locus": "GCA003260355_00448" }, { "type": "cds", "sequence": "contig_1", "start": 233097, "stop": 233801, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBF9D7A", "UniRef:UniRef100_A0A660N036", "UniRef:UniRef50_A0A288DHQ7", "UniRef:UniRef90_A0A660N036" ], "nt": "ATGTTCTTCAAAAACCTTGAAAATCAAAATATCCAAAAAGGGAAACAAGAAACTCAGTTTAATACCGAGACAATGCTTCCTATTTCTGAGATCAAGGACGGATACGCAATTCTCAAAGACGGAGGAATCAGAGCAATCCTGAAGGCAGACGGCGTCAACCTCGATCTCAAAAACTTTGACGAAATCCAAACCATCTTAGAGCAATACAAAAGATTTCTCAATGGACTAGAGTTTCCCTTACAGTTTCTGATCCGTAATACCTACCTAGACCTGAGAGAATATCTCAATTACGTGCACGAAAACATCAATAAACTAGACCCGGGAGCTCTAAAAGAGCAAGGAAAAAATTATGCGTCTTTTTTGGAAGAGATCAACCTGAGACAAGGGCTTATTTTCGTCAAGGAATTTTATATCATCATTCCTTACTACACGAGCGAAACTGATCTCGACCAAGTAAAAAAACCGCGACGAAGCAAGTTTATGGATACTCTTAATAGCAAGGACGACATCGAAACCATCGTTTCCAGATATAGGAACTTCATAAAAGGGCAAAAAATGCTCAACACAAGAGTTGCCCTTATCCAAGAAGGTCTCGGTAGCCTCGGCATCAAAACCGAACTCCTCAATACGAGAGATCTCATTTCGCTCCTCTTTTCGATTTACAATCCCCTCCTCGATTCCACACAAAGCGTTAGCAATGAATAA", "aa": "MFFKNLENQNIQKGKQETQFNTETMLPISEIKDGYAILKDGGIRAILKADGVNLDLKNFDEIQTILEQYKRFLNGLEFPLQFLIRNTYLDLREYLNYVHENINKLDPGALKEQGKNYASFLEEINLRQGLIFVKEFYIIIPYYTSETDLDQVKKPRRSKFMDTLNSKDDIETIVSRYRNFIKGQKMLNTRVALIQEGLGSLGIKTELLNTRDLISLLFSIYNPLLDSTQSVSNE", "aa_hexdigest": "e8d0cf54681c7767506700cd459e267f", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000DBF9D7A", "uniref100_id": "UniRef100_A0A660N036", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBF9D7A", "UniRef:UniRef100_A0A660N036" ] }, "ips": { "uniref100_id": "UniRef100_A0A660N036", "uniref90_id": "UniRef90_A0A660N036" }, "psc": { "uniref90_id": "UniRef90_A0A660N036", "uniref50_id": "UniRef50_A0A288DHQ7" }, "pscc": { "uniref50_id": "UniRef50_A0A288DHQ7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A288DHQ7" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 27224.85540000003, "isoelectric_point": 5.692391014099121 }, "id": "NKOCPLAKPK_486", "locus": "GCA003260355_00449" }, { "type": "cds", "sequence": "contig_1", "start": 233845, "stop": 234294, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MZE5", "UniRef:UniRef90_A0A660MZE5" ], "nt": "TTGATAACAAGCATTTTCGCATGCATTTCTTTTGCGCCCATTTTTGCAGAGGAGAACCCGTTTTACTATGAAGGAGGAGTCATAGGAACAGATACTGATACTGAATACGGAAAGCTCGTAAAAGACGATGCTGTAAGCCCAGAAGATAGCCTGCTAAATAGATTGCTCAGTGTGTTTAGCCTTGATGGTCCAGATTATCACGGACCACAGAAAGCCTTTTACTATATCAAAAAAATTCTGAATTATGCACTCAGTTTTGTAAGTCTTATTGCATTTTGTCTTCTTTTGTATAGTTTCTATGGTATGGTGGTTGGAGACGGAGATAAACAATTTAACAACGTAAAAAGCACCCTAAAAGGAATCGCCATCGCTATCGCAGCTATGGGCTTAGCACGAATGATCGTGACACTCCTCTTCCGAATCTATCAAAACAAAGCATATACACCGTAG", "aa": "MITSIFACISFAPIFAEENPFYYEGGVIGTDTDTEYGKLVKDDAVSPEDSLLNRLLSVFSLDGPDYHGPQKAFYYIKKILNYALSFVSLIAFCLLLYSFYGMVVGDGDKQFNNVKSTLKGIAIAIAAMGLARMIVTLLFRIYQNKAYTP", "aa_hexdigest": "3faac0a220f8560572e44a7b5fd5ca51", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A660MZE5", "query_cov": 1.0, "subject_cov": 0.9371069182389937, "identity": 0.993, "score": 294.0, "evalue": 8.91e-101, "valid": true, "uniref50_id": "UniRef50_A0A660MZE5" }, "pscc": { "uniref50_id": "UniRef50_A0A660MZE5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MZE5" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16559.02689999999, "isoelectric_point": 5.271896171569824 }, "id": "NKOCPLAKPK_487", "locus": "GCA003260355_00450" }, { "type": "cds", "sequence": "contig_1", "start": 234435, "stop": 234554, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTAGTCTGGTCGCTTTTTGCATATTACTCTATGCTGGCTTCCAAATGACAACAGCCGCCGGAGACGATGGAAAATTTAAGGCAGGAAATAAAACCCTCAAAAAAATAGCAATCTGA", "aa": "MLSLVAFCILLYAGFQMTTAAGDDGKFKAGNKTLKKIAI", "aa_hexdigest": "7167f39a097eea9c5fa914b87dba1413", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4150.9663, "isoelectric_point": 9.430773735046387 }, "id": "NKOCPLAKPK_488", "locus": "GCA003260355_00451" }, { "type": "cds", "sequence": "contig_1", "start": 234696, "stop": 234854, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCGAAAGTATGGAATTTTAGGATTGTGTAGTATGCTCATCTTGGGGATAAACTTTTCTACAGCCACAAGATCTCTCGGTCCCACAACGGACCAAATCCTCACTACTCAAGACAAAGACATCATCACTCCTCCCGACCAAGAAGCAATGCGTCAGTGA", "aa": "MRKYGILGLCSMLILGINFSTATRSLGPTTDQILTTQDKDIITPPDQEAMRQ", "aa_hexdigest": "f479370329980af64020e40c9906ec08", "start_type": "ATG", "rbs_motif": "TTTA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5754.634900000002, "isoelectric_point": 5.953679847717285 }, "id": "NKOCPLAKPK_489", "locus": "GCA003260355_00452" }, { "type": "cds", "sequence": "contig_1", "start": 234969, "stop": 235241, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02887.21" ], "nt": "TTGGGTCCAAAGATACCAATCATCACCTTTACCCAATCAAAAGATACCTACAGATACTTAAGTCTCGTGCGAGGAGTAAAAGGATACAAAATCTCACAATCCTTCAACTACGAGAATCTCAAAAAAATCGGAAAAGAAATGATTAGAATGATCTTCAAGGGGAACATTTCTCTTGATGATAAAATCCTCATTCTCCAGGCAAACGAAACTCTCAATCCCGTAAAAACCGATATGATCAATGGAATTGAGTTTTACAATTTTAAAAACATCTAA", "aa": "MGPKIPIITFTQSKDTYRYLSLVRGVKGYKISQSFNYENLKKIGKEMIRMIFKGNISLDDKILILQANETLNPVKTDMINGIEFYNFKNI", "aa_hexdigest": "759f5f1a164319d1415d940b4a116cdc", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02887.21", "name": "PK_C", "length": 78, "aa_cov": 0.8555555555555555, "hmm_cov": 0.9615384615384616, "evalue": 1.579608480370549e-08, "score": 31.138158798217773, "start": 3, "stop": 79 } ], "seq_stats": { "molecular_weight": 10453.18299999999, "isoelectric_point": 9.65615520477295 }, "id": "NKOCPLAKPK_490", "locus": "GCA003260355_00453" }, { "type": "cds", "sequence": "contig_1", "start": 235413, "stop": 235640, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATCCAGCCAAGAAACTAAACACATTGATCCAGTCAGTCAAAGCCGAAGGAAAACCTGTGCTTATCACCTATACTAATAGAGATTACGAAAAACCTTTTCTGAAATCTTTTGGGAAAGAGATGCAAGAATTAGCTCAGGAACATATTGACGGAATCATGCTAGAGACCTTCCTTATTGAGGATCAGGTTTTTGATGTAATTGAAAAAGCAGGAGATATCCTCTGA", "aa": "MNPAKKLNTLIQSVKAEGKPVLITYTNRDYEKPFLKSFGKEMQELAQEHIDGIMLETFLIEDQVFDVIEKAGDIL", "aa_hexdigest": "91fd019bb95e8637a0a7c2a6fbe855fd", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8584.842499999995, "isoelectric_point": 4.8377599716186515 }, "id": "NKOCPLAKPK_491", "locus": "GCA003260355_00454" }, { "type": "cds", "sequence": "contig_1", "start": 235710, "stop": 235931, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00224.27" ], "nt": "TTGCAATACGATAGAATAAATTTTGATGCCTTAGATAACAAAGATGAAGCCCTAACAGAAAAGGATAAAAAAGACATCCTCTGGGGACTAGAATACGGAGCTCACGTCATCGGACTTGCCTGCTCCAATAGTGCCGAACATGTAGAAAGTGCAAAAGAATTCCTTGATCAAAATAATGCGAAAGAAATGAAAGTTTTCGCCAAGATAGAAACCACGTCTTGA", "aa": "MQYDRINFDALDNKDEALTEKDKKDILWGLEYGAHVIGLACSNSAEHVESAKEFLDQNNAKEMKVFAKIETTS", "aa_hexdigest": "a2db4b20d2003d1378adfc29b523f17f", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00224.27", "name": "PK", "length": 66, "aa_cov": 0.9041095890410958, "hmm_cov": 0.9848484848484849, "evalue": 1.552780481643486e-09, "score": 33.76520919799805, "start": 6, "stop": 71 } ], "seq_stats": { "molecular_weight": 8279.132799999998, "isoelectric_point": 4.717374992370605 }, "id": "NKOCPLAKPK_492", "locus": "GCA003260355_00455" }, { "type": "cds", "sequence": "contig_1", "start": 235938, "stop": 236375, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00224.27" ], "nt": "ATGAACAACTTTAGATTTACCAAAATCATTGCTGGAGTAAGTCCGATCCTTGCTAAAGAAACCGTGCTTTCTAAAATCATCAATATGGTAGATGCCTTTAGAATTACCCTCTCAAAAGGCTATGACGACAATAACAAAAAATACATTGATACCATCATGAAGCTTGACAACAGCAAAACAATCATCTTGGAAACCAAAGGGATTGATATCAGAATGAAAAACACCTGCAATTTTCCAATCAAAAAGGGAGAAAAATGGACCATGGAGTATTCTGAATACGCACAAGAAGGAACGAGTAAAATTTACATCGACTATCCAGCCCTCGGAAATTTGAAGGAAAATACGATCATCACCTGTGAACAGAGCAACACGAGCTTTAAAATTCTGAGCACAGAAGAGGATACCGCACAAGTCGAAGTTTTATCAGCAGGAAACTGA", "aa": "MNNFRFTKIIAGVSPILAKETVLSKIINMVDAFRITLSKGYDDNNKKYIDTIMKLDNSKTIILETKGIDIRMKNTCNFPIKKGEKWTMEYSEYAQEGTSKIYIDYPALGNLKENTIITCEQSNTSFKILSTEEDTAQVEVLSAGN", "aa_hexdigest": "d3be5f05390cdce5e3ca9af0fbd0303b", "start_type": "ATG", "rbs_motif": "TTTA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00224.27", "name": "PK", "length": 152, "aa_cov": 0.9586206896551724, "hmm_cov": 1.0, "evalue": 4.181861569680371e-11, "score": 38.9244499206543, "start": 5, "stop": 143 } ], "seq_stats": { "molecular_weight": 16420.67289999999, "isoelectric_point": 6.331829643249511 }, "id": "NKOCPLAKPK_493", "locus": "GCA003260355_00456" }, { "type": "cds", "sequence": "contig_1", "start": 236437, "stop": 236649, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAAGGTAGATATTGCAGGAGGCGATAGGAAAGGCGATCCTTTGGTGATTTCGCTTCCAGAAGAGTTTAGAAATAAAACGGTTCCACTGCTCGTTGATGATGGATTAGTCTATGCAAAGGCGGATAATCAAGCAAACATTGTCATCAAAAATAAAGAAACTGCTACACTCATCAAGAGAAAAGGCTTTGTCGTAGTCAATACTGAAGGATAA", "aa": "MKVDIAGGDRKGDPLVISLPEEFRNKTVPLLVDDGLVYAKADNQANIVIKNKETATLIKRKGFVVVNTEG", "aa_hexdigest": "be37a96e5dbbf729b10c16a92c2c7591", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7623.738600000003, "isoelectric_point": 8.008343315124513 }, "id": "NKOCPLAKPK_494", "locus": "GCA003260355_00457" }, { "type": "cds", "sequence": "contig_1", "start": 236728, "stop": 237555, "strand": "-", "frame": 1, "gene": null, "product": "Bacterial type II secretion system protein E domain-containing protein", "db_xrefs": [ "KEGG:K06865", "SO:0001217", "UniRef:UniRef50_A0A288DGL8", "UniRef:UniRef90_A0A328EG99" ], "nt": "GTGAAACCGGTAAAGAAAATGAGCATTGAAGAGTATAATCTCCAGCCAGAACTCTTTGAACTCTTGAGGAATAAATCTAAAGGAATCCTCGTTTCTGGAGCTCCTGGAAGCGGAAAATCTACTTTTACTGGGGCTTTGATTGAGCTATATCACAAAGATCAGCATATCATCAAGACGATTGAGTCGCCAAGAGATTTGATGCTGAATGATGATATTGTGCAGTATTCCTTTACCTACGGAAGCCACGATGAAGTGAGGGATATTCTTCTGCTTTCTAGACCTGATTATACGATTTATGATGAAGTAAGAAATAAACCTGACTTTGAACTCTATAAGGATCTCAGACTCACAGGAATTGGACTCGTTGGAGTCATCCATGCGACGAAACCGGTAGATAGTATTCAGAGATTCATCGGAAGTATTGAAATGGGAATCATTCCTCAAGTCGTAGATACCGTTATCTTTATTGATAAAGGGCAAGTGAGTGAAGTCCTAACCCTAGAACTTACTGCAAAAGTCCCAGATGGAATGCTTTCAGAAGAGCTTGCGAGACCAGTCATTGTCGTTTCTAGCTTCCTTCAGAAAAAGTCACTTTATGAAATCTATACCTTTGGAGAGCAAGTCGTGGTAATGCCAATTGCTACCGATGAAAATGGCAATCCAAAAGTACCAACCAAAACCCAAGTCGTTTCTCAGTATGCAAAAGAGGGAATTCAGAGAAAATTACAGCAGTTGCTCCCTTGTGATTTCCATATTGCGATCAAAGGAAGCGAGCTTGAGCTTTATATTCCTGAATACTATAAAGGAAAAATTATCGGAAAAGGCTGA", "aa": "MKPVKKMSIEEYNLQPELFELLRNKSKGILVSGAPGSGKSTFTGALIELYHKDQHIIKTIESPRDLMLNDDIVQYSFTYGSHDEVRDILLLSRPDYTIYDEVRNKPDFELYKDLRLTGIGLVGVIHATKPVDSIQRFIGSIEMGIIPQVVDTVIFIDKGQVSEVLTLELTAKVPDGMLSEELARPVIVVSSFLQKKSLYEIYTFGEQVVVMPIATDENGNPKVPTKTQVVSQYAKEGIQRKLQQLLPCDFHIAIKGSELELYIPEYYKGKIIGKG", "aa_hexdigest": "6c008d2858e3e6fe9d24d36a636e136e", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 236437, "stop": 237855, "observations": { "mutation": [ 236730, 237615 ], "stop": [ 236730, 237615 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EG99", "query_cov": 1.0, "subject_cov": 0.5753138075313807, "identity": 0.993, "score": 534.0, "evalue": 1.49e-188, "gene_start": 1, "gene_end": 275, "reference_start": 107, "reference_end": 381, "reference_sequence": "MTQTIIGNSVLDKYFDETTMSLHLKVNRPILRKKGFPGNWQPVVDPNEIMTKEAFNELIDQLFKEVESREDAFLEINRELSKVLQIGPYRIVIVYPALSDGLELTAVKPVKKMSIEEYNLQPELFELLRNKSKGILVSGAPGSGKSTFTGALIELYHKDQHIIKTIESPRDLMLNDDIVQYSFTYGSHDEVRDILLLSRPDYTIYDEVRNKPDFELYKDLRLTGIGLVGVIHATKPVDSIQRFIGSIEMGIIPQVVDTVIFIDKGQVSEVLTLELTAKVPDGMLSEELARPVIVVSSFLQKKPLYEIYTFGEQVVVMPIATDENGNPKVPTKTQVVSQYAKEGIQRKLQQLLPCDFHIAIKGSELELYIPEYYKGKIIGKGGTAITNLEKDIGLRIHVKTFNELPLLEVKVDIAGGDRKGDPLVISLPEEFRNKTVPLLVDDGLVYAKADNQANIVIKNKETATLIKRKGFVVVNTEG" }, "paralog": false, "identity": 0.989406779661017, "score": 914.0, "evalue": 0.0, "description": "Internal stop codon at 236730, 237615. Nonsense mutation around 236730, 237615.", "psc": { "uniref90_id": "UniRef90_A0A328EG99", "product": "Bacterial type II secretion system protein E domain-containing protein", "uniref50_id": "UniRef50_A0A288DGL8", "kegg_orthology_id": "K06865", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EG99", "UniRef:UniRef50_A0A288DGL8", "KEGG:K06865" ] }, "pscc": { "uniref50_id": "UniRef50_A0A288DGL8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A288DGL8" ], "product": "ATPase" } }, "id": "NKOCPLAKPK_495", "locus": "GCA003260355_00458" }, { "type": "cds", "sequence": "contig_1", "start": 237613, "stop": 237873, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACGCAAACAATTATTGGAAACTCAGTCTTGGACAAATATTTTGACGAAACGACTATGTCTCTCCATCTCAAAGCTAATAGACCAATTCTCAGGAAAAAAGGATTCCCAGGGAATTGGCAACCTGTCGTGGATCCAAATGAAATTATGACCAAAGAAGCCTTTAATGAACTGATTGATCAGCTTTTTAAAGAGGTAGAAAGCAGAGAAGATGCTTTTTTAGAGATCAATAGAGAACTCTCTAAAGTCCTTCAAATCTGA", "aa": "MTQTIIGNSVLDKYFDETTMSLHLKANRPILRKKGFPGNWQPVVDPNEIMTKEAFNELIDQLFKEVESREDAFLEINRELSKVLQI", "aa_hexdigest": "e86e2d7e2320793d3e0cd15b9a32b408", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10023.392699999997, "isoelectric_point": 5.08932933807373 }, "id": "NKOCPLAKPK_496", "locus": "GCA003260355_00459" }, { "type": "cds", "sequence": "contig_1", "start": 238060, "stop": 238605, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EJ90", "UniRef:UniRef90_A0A328EJ90" ], "nt": "ATGTTTTTTCATTTAACGGGAATAAAAGATATTGCAGATAGAAATTATAGCTTGGAGTTTCTCATTGAGGGAGAAGATATATCGTCTGTCAAGCAATTCCTTGCTGATCAGAAGGTTATTGTTCTTGGATTGGAGCATTTTACCAATGAACCTCAGACTTTTGGAGCTGTTTATTTGGATGTTGAGTCTAAGAAGAAGGTTTTTAGGATTGTTGGGAAGTTTGATACGTTAGAAGAATTTCTTGCTTACTTTGTGAAACTAAAACTTAAAGTCGTAGATGCTAATTCTTTTGTTTCTCCTCTAGAGCCTCAAAAAGTTCAATCTCTCCTTCAACAGATTTATCAGAAACAAGAAGAGCAAGAACGTCTCTATCAACAGGCCAAGGAAAAAAAGGCAAAACAGGCAAAATTAAATTTTCAAGATAAGAAGCTAGCTAGGGCATATGAGGCGATTGACCAGATTATTAGTCAAATTGATCAGCTTTTGGCTATTGGTGGGAAAAATATTCCTCCTATGACGTTGAAAAATTTTGATGATATGAGGTGA", "aa": "MFFHLTGIKDIADRNYSLEFLIEGEDISSVKQFLADQKVIVLGLEHFTNEPQTFGAVYLDVESKKKVFRIVGKFDTLEEFLAYFVKLKLKVVDANSFVSPLEPQKVQSLLQQIYQKQEEQERLYQQAKEKKAKQAKLNFQDKKLARAYEAIDQIISQIDQLLAIGGKNIPPMTLKNFDDMR", "aa_hexdigest": "59a5868f11f07c5eb8ec315159bbee40", "start_type": "ATG", "rbs_motif": "TTTA", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 238060, "stop": 238908, "observations": { "mutation": [ 238603, 238705, 238720 ], "stop": [ 238603, 238705, 238720 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EJ90", "query_cov": 1.0, "subject_cov": 0.4738219895287958, "identity": 0.9890000000000001, "score": 340.0, "evalue": 4.91e-115, "gene_start": 1, "gene_end": 181, "reference_start": 1, "reference_end": 181, "reference_sequence": "MFFHLTGIKDIADRNYSLEFLIEGEDISSVKQFLADQKVIVLGLEHFTNEPQTFGAVYLDVESKKKVFRIVGKFDTLEEFLAYFVKLQLKIVDANSFVSPLEPQKVQSLLQQIYQKQEEQERLYQQAKEKKAKQAKLNFQDKKLARAYEAIDQIISQIDQLLAIGGKNIPPMTLKNFDDMRGAISKLRLATNYDKIIEELHKAMNLIVETQDLLLGKLDAGKIFTVVPQSTITNIEVIREQTRLAKARLLTALGAQLSGDEVMYSSLGYLKTFTQYLRKDIRTAMDSKLTLCRLVFQGAELLLLFFLLEVVIFSVFDQNFGLNLGVQRVGIILIYGGVLAGLLRIVNEYFRPQTVLYYLFFFLGIVAIYFLIIYLIKLFLVL" }, "paralog": false, "identity": 0.9716312056737588, "score": 525.0, "evalue": 4.82e-190, "description": "Internal stop codon at 238603, 238705, 238720. Nonsense mutation around 238603, 238705, 238720.", "psc": { "uniref90_id": "UniRef90_A0A328EJ90", "uniref50_id": "UniRef50_A0A328EJ90", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EJ90", "UniRef:UniRef50_A0A328EJ90" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EJ90", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EJ90" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 20965.964699999997, "isoelectric_point": 6.757098960876465 }, "id": "NKOCPLAKPK_497", "locus": "GCA003260355_00460" }, { "type": "cds", "sequence": "contig_1", "start": 238804, "stop": 239208, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTTGCTGCATTGGGAGCTCAGCTTTCAGGAGATGAAGTAATGTATTCTTCACTTGGGTATCTTAAAACTTTTACTCAATATCTTCGTAAGGATATTCGTACGGCGATGGATAGTAAGTTAACTTTGTGTAGATTGGTTTTTCAGGGAGCAGAATTATTGTTGTTGTTTTTCCTCTTGGAGGTTGTGATTTTTTCTGTTTTTGGTCAGAATTTTGGTCTTAATTTAGGTGTTCAAAGGGTTGGAATTATCCTGATATATGGAGGTGTTTTGGCGGGACTTTTGAGAATTGTGAATGAGTATTTTCGTCCTCAAACGGTTTTGTATTATCTGTTCTTTTCCCTCGGTATTGTTGCTATTTATTTTTTTATTATCTATCTTATCAAATTATTTTTAGTTCTCTAG", "aa": "MLAALGAQLSGDEVMYSSLGYLKTFTQYLRKDIRTAMDSKLTLCRLVFQGAELLLLFFLLEVVIFSVFGQNFGLNLGVQRVGIILIYGGVLAGLLRIVNEYFRPQTVLYYLFFSLGIVAIYFFIIYLIKLFLVL", "aa_hexdigest": "326b8cb9ab71e6cc756d82a29eaef421", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15229.14679999999, "isoelectric_point": 9.097085380554201 }, "id": "NKOCPLAKPK_498", "locus": "GCA003260355_00461" }, { "type": "cds", "sequence": "contig_1", "start": 239747, "stop": 240406, "strand": "+", "frame": 2, "gene": null, "product": "Polysaccharide biosynthesis protein C-terminal domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EJH7", "UniRef:UniRef90_A0A328EJH7" ], "nt": "TTGGCTTTTCTCCGTGCTCTTTTCGTTAGTGTGAATGGAGGACTTCAAGCATTGAACTTGGATTTGGGAATTTGCTTTGTTTTGCTCCTTTGTTTGGATTATTCCAAGTCTTCTCCGCAGAAAAATCTTGAAGCCTCTAGGAATGTTTCACTTAGGAGGGTTTTGGCAGGGGAGAGGATTTTGGGCAAAAAAAATAGACTCACTCTGATGCACTCAGGAATTCTTTTTTTCAAGGAGTTGCCAATGAAACTTTGGAACTTTTTGGAGTATCTGAATGTAGTTCTTTTGCTAGGAGTCCTTGTTGCTTATCTCTTTCCTGTGTTTCAGGGGCAGGTTTTGCAGCAGTGGTGGTATCGATTGGGGATTGCGTTTTTCTTAGCTAATGCTTTTTTGCTGAAGAAGAATCAGATTTTTTCTCTTTTGAGTAGATTTGCTGTGGTTGTGATTATTAATTTTTCTTTGTATGTGAGTTTATTGGTCGTGGATAAGAATATACAGTCAATGCTTCCTTGGCTTATCGTTTGGAATCTGCTCTGTGGAGTGCTTGTTTTTTATACAAGATTTGGCATGGTAAGGAAATATGTGAAGAAAATTGATGTGCTTTTCTGGCTGATTGCAAGTCTCGTGGCGATGTTGCTTAATATTGTGCTTCTTATGTGA", "aa": "MAFLRALFVSVNGGLQALNLDLGICFVLLLCLDYSKSSPQKNLEASRNVSLRRVLAGERILGKKNRLTLMHSGILFFKELPMKLWNFLEYLNVVLLLGVLVAYLFPVFQGQVLQQWWYRLGIAFFLANAFLLKKNQIFSLLSRFAVVVIINFSLYVSLLVVDKNIQSMLPWLIVWNLLCGVLVFYTRFGMVRKYVKKIDVLFWLIASLVAMLLNIVLLM", "aa_hexdigest": "7f12f7e87dfcb9a92ebf513077be6e83", "start_type": "TTG", "rbs_motif": "TTTTT", "genes": [], "truncated": "both", "pseudogene": { "start": 239447, "stop": 240583, "observations": { "mutation": [ 239588, 239684, 240404, 240419, 240464 ], "stop": [ 239588, 239684, 240404, 240419, 240464 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EJH7", "query_cov": 1.0, "subject_cov": 0.48237885462555063, "identity": 0.995, "score": 408.0, "evalue": 4.69e-140, "gene_start": 1, "gene_end": 219, "reference_start": 177, "reference_end": 395, "reference_sequence": "MIYFIVLLICGAFIAFFLQLGVWYFGLFPLIFLVVFFSQGIKDVHLKLLETDLSEKYGLYLLIGFIFLGIFGVLNFIGLDYQSGFFVLLFLTLLARYGAYFLNYEDGKQLFEHFFLIFLLLLLGNSVFLEGVIGVSAMLSLTSLFAVLAYGGLRYGVASFFPVDSKYQYYFFFFLFLAFLRALFVSVNGGLQALNLDLGICFVLLLCLDYSKSSPQKNLEASRNVSLRRVLAGERILGKKNRLTLMHSGILFFKELPMKLWNFLEYLNVVLLLGVLVAYLFPVFQGQVLQQWWYRLGIAFFLANAFLLKKNQIFSLLSRFAVVVIINFSLYVSLLVVDKNIQSMLPWLIVWNLLCGVLVFYTRFGMVRKYVKKIDVLFWLIASLVAMLLNIVLLMGLSISGQLIFSLIFFYLGIQGVITYYVIQLIKVYDLPKTEEGSTSSDPLEKLLEKEISL" }, "paralog": false, "identity": 0.9735449735449735, "score": 697.0, "evalue": 1.39e-256, "description": "Internal stop codon at 239588, 239684, 240404, 240419, 240464. Nonsense mutation around 239588, 239684, 240404, 240419, 240464.", "psc": { "uniref90_id": "UniRef90_A0A328EJH7", "product": "Polysaccharide biosynthesis protein C-terminal domain-containing protein", "uniref50_id": "UniRef50_A0A328EJH7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EJH7", "UniRef:UniRef50_A0A328EJH7" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EJH7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EJH7" ], "product": "Polysaccharide biosynthesis protein C-terminal domain-containing protein" } }, "id": "NKOCPLAKPK_499", "locus": "GCA003260355_00462" }, { "type": "cds", "sequence": "contig_1", "start": 240467, "stop": 240583, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATTACTTATTATGTCATTCAACTGATAAAAGTGTATGATTTACCAAAGACTGAGGAAGGATCAGCTAATTCTGACCCTCTTGAGAAACTTTTGGAGAAGGAAATTTCTCTTTAA", "aa": "MITYYVIQLIKVYDLPKTEEGSANSDPLEKLLEKEISL", "aa_hexdigest": "21a606f35669482631905406a2838eaf", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4385.033999999999, "isoelectric_point": 4.4943956375122065 }, "id": "NKOCPLAKPK_500", "locus": "GCA003260355_00463" }, { "type": "cds", "sequence": "contig_1", "start": 240589, "stop": 240795, "strand": "-", "frame": 1, "gene": "ftsL", "product": "Cell division protein FtsL", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EG62", "UniRef:UniRef90_A0A328EG62" ], "nt": "ATGTATTACATTAATTTTTCGACCACAAGAGGATATTTTATGAAACTCGCAAATCAGGAACTCTACTCAGCAAAAGCAAAAAGCGATATTCTAAAATTAGAAGTCCTGAGAGAAAAAAGAGAAAACCGAGATAATCTTTATACTCCAAAAAATTCTGACCTCTCCAAAAAACAAATCATTATCATTATCCCAGAAGAGAGTAAATAA", "aa": "MYYINFSTTRGYFMKLANQELYSAKAKSDILKLEVLREKRENRDNLYTPKNSDLSKKQIIIIIPEESK", "aa_hexdigest": "b0e7fb0e4045d04f214c56af233e8627", "start_type": "ATG", "rbs_motif": null, "genes": [ "ftsL" ], "truncated": "3-prime", "pseudogene": { "start": 240589, "stop": 240984, "observations": { "mutation": [ 240807, 240837 ], "stop": [ 240807, 240837 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EG62", "query_cov": 1.0, "subject_cov": 0.5190839694656488, "identity": 0.985, "score": 128.0, "evalue": 6.14e-37, "gene_start": 1, "gene_end": 68, "reference_start": 64, "reference_end": 131, "reference_sequence": "MPENNTSFQIAQHKVKTFAYQSLKSFHKSFKSFLETYRTKQELRDLAKIGGLLAVFLLFGGIFMYYINFSTTRGYFMKLANQELYSAKAKSDILKLEVLREKRENRDNLYTPKNSDLSKKPIIIIIPEESK" }, "paralog": false, "identity": 0.9770992366412213, "score": 249.0, "evalue": 7.35e-87, "description": "Internal stop codon at 240807, 240837. Nonsense mutation around 240807, 240837.", "psc": { "uniref90_id": "UniRef90_A0A328EG62", "product": "Cell division protein FtsL", "uniref50_id": "UniRef50_A0A328EG62", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EG62", "UniRef:UniRef50_A0A328EG62" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EG62", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EG62" ], "product": "Cell division protein FtsL" } }, "id": "NKOCPLAKPK_501", "locus": "GCA003260355_00464" }, { "type": "cds", "sequence": "contig_1", "start": 240835, "stop": 240984, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCAGAGAATAACACCTCTTTCCAAATAGCTCAGCATAAAGTAAAAACTTTCGCCTATCAATCATTAAAATCATTTCACAAAAGCTTCAAAAGTTTCCTCGAAACCTACAGAACTAAACAAGAATTACGAGATCTAGCAAAAATCTGA", "aa": "MPENNTSFQIAQHKVKTFAYQSLKSFHKSFKSFLETYRTKQELRDLAKI", "aa_hexdigest": "e09237a28a9ec7f27c7c953f96e6e6fc", "start_type": "ATG", "rbs_motif": "TTATT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5866.661799999999, "isoelectric_point": 9.90029697418213 }, "id": "NKOCPLAKPK_502", "locus": "GCA003260355_00465" }, { "type": "cds", "sequence": "contig_1", "start": 241196, "stop": 241519, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGATTTGATCAGGAAAAGAATCCGACGACCGCAAAAGATTGGCTTCAAAAAACAAAACCCGAAACCATTGAACAAGCCTTACAAATCTATGGAGACTTCTCTCCCAAAACCGCTGAATGGCTCACCAAACTCCTCTGTGAACAAAGAAAAAAATCTGAATTTTCAACTACTTTTCAGCTCAAAGACTTTCTTACCAGCCATCATTTCAATCAGAAAAAGATTGCCGTCATTTTCCAAGTCATCAGAATTATGGTCAATCGTGAACTTGAACAGTTAGAGCAGTTTTTAGAAGACTTTCCTCAGACTTTGAAAAAGGGTTGA", "aa": "MRFDQEKNPTTAKDWLQKTKPETIEQALQIYGDFSPKTAEWLTKLLCEQRKKSEFSTTFQLKDFLTSHHFNQKKIAVIFQVIRIMVNRELEQLEQFLEDFPQTLKKG", "aa_hexdigest": "87188b69ac769e3607b4a754f72ebff0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12794.570099999986, "isoelectric_point": 8.709759330749513 }, "id": "NKOCPLAKPK_503", "locus": "GCA003260355_00466" }, { "type": "cds", "sequence": "contig_1", "start": 241616, "stop": 241897, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTCCGCCATATTCCTGTCCTCGCCCAAGAAATCTACGATCTGCTCCCACCAAACCGACAACTTGGCTTTGATGGAACTTTTGGACATGGAGGACATGCGGAGTTTTTCCTCAGCCAAGAAGAGCAGAAAAGACCGATTGAAACATTAAAAATCATCGGAACTGATATTGACCAGCAGATGATCCAGAAAGCGATAGAGCTAACCAAAACCTACGCTCATCAAATCAAAATTCTCCATACTTCATATGCCAAAATTAAGGATATTAGCAAAGAAAACTGA", "aa": "MLRHIPVLAQEIYDLLPPNRQLGFDGTFGHGGHAEFFLSQEEQKRPIETLKIIGTDIDQQMIQKAIELTKTYAHQIKILHTSYAKIKDISKEN", "aa_hexdigest": "d117d639df1f2274f7bdaa9c41baefb8", "start_type": "ATG", "rbs_motif": "TTTA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10700.181899999987, "isoelectric_point": 6.498765754699707 }, "id": "NKOCPLAKPK_504", "locus": "GCA003260355_00467" }, { "type": "cds", "sequence": "contig_1", "start": 241997, "stop": 242110, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTTTGGAAGTAGGAATAAAAACCTTTAATCTCTGGCATCCAGAAGGGATTGTCGCTTATGATACGGAAGAATATAATATCCTTTCCTACCAATATCAGGGAGAAAAGTAA", "aa": "MALEVGIKTFNLWHPEGIVAYDTEEYNILSYQYQGEK", "aa_hexdigest": "89945b39ccdae1b7ba4ad93979477c29", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4350.810300000002, "isoelectric_point": 4.413115882873536 }, "id": "NKOCPLAKPK_505", "locus": "GCA003260355_00468" }, { "type": "cds", "sequence": "contig_1", "start": 242730, "stop": 243812, "strand": "-", "frame": 2, "gene": null, "product": "Elongation factor G", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B5JXT8" ], "nt": "TTGCTTAATTCTATTACTGGAAAGAAAGAAAGAGTAGGAAGACTTCTTCTGATGCACGCAAACAAGAGAGAAGAAATTTCTGAAATTTCAGCTGGAAATATCTGTGCTTTCCTCGGACTTAAAGAAACTCAAACAGGACACACGCTTTGTGATGCTGCAAAACCTATCGTTCTTGAAAAAATGGAATTCCCTGATCCAGTGATCCATATTGCTATTGAACCAAAATCAAAAGCTGATCAAGAAAAGCTTGGTTTGGCTCTTTCAAAACTTATGGCTGAAGATCCTTCTTTCACTGCTCATTCTGATGAAGAAAGTGGGCAAACTGTGATCGGAGGTATGGGTGAGCTTCATCTTGATGTGATCGTCGATAGACTCAAGAGAGAGCATAAAGTAGAAGTAAATACTGGTAAACCTCAAGTAGCGTATAGAGAAACTATTTTCGCTACTGCAGAAGGAGAAGGACTCTTCAAGAAACAATCTGGAGGTAGAGGTCAGTATGGACACGTAGTTCTTAGACTAGAAAAACTTCCAGAAGAGAAAAACTACGAATTTGTATCAGAAATTAAAGGTGGAGTAATCCCTAACGAGTTTATCCCTGCTATTGATAAGGGAGCAAAAGAAACCATGGCTCAAGGTATCCTTGCTGGATTCCCTATCATCAATATTAAGGTAGTTCCTTTCTTTGGATCTTATCATGATGTGGATTCTTCAGAAGTTGCGTTTAAAGTTGCCACTTATAAGGCTTTCAAAGAGGCTTTCGCAAAAGCATCTCCTGCCATTCTTGAACCAATCATGTCTGTAGAAATCGTAACTCCAGAAGAATACGTAGGAACCGTAATGGGAGATCTTTCATCTAGGAGAGGTATCATCCTCGGACAAACTCCAAGAGGAAATGCTACAGCAATTGCTGCAAAAGTTCCACTTTCAGAGATGTTTGGATATGCGACTGATTTGAGATCAAATACTCAAGGAAGAGCTGCATACTCTATGGAGTTCTCAAACTATGAAAAGGTTCCTGCAATGATTGAAAAGAAAATCATCGAAGAAAGAGCAGGAAAGGTAAAGAAAATGGATGAAGAATAA", "aa": "MLNSITGKKERVGRLLLMHANKREEISEISAGNICAFLGLKETQTGHTLCDAAKPIVLEKMEFPDPVIHIAIEPKSKADQEKLGLALSKLMAEDPSFTAHSDEESGQTVIGGMGELHLDVIVDRLKREHKVEVNTGKPQVAYRETIFATAEGEGLFKKQSGGRGQYGHVVLRLEKLPEEKNYEFVSEIKGGVIPNEFIPAIDKGAKETMAQGILAGFPIINIKVVPFFGSYHDVDSSEVAFKVATYKAFKEAFAKASPAILEPIMSVEIVTPEEYVGTVMGDLSSRRGIILGQTPRGNATAIAAKVPLSEMFGYATDLRSNTQGRAAYSMEFSNYEKVPAMIEKKIIEERAGKVKKMDEE", "aa_hexdigest": "61cc5fdbe83d9c9e6a6a217e989da4fe", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_B5JXT8", "query_cov": 0.9888888888888889, "subject_cov": 0.9270833333333334, "identity": 0.612, "score": 436.0, "evalue": 6.33e-150, "valid": false, "gene": "fusA", "product": "elongation factor G", "uniref50_id": "UniRef50_B5JXT8", "kegg_orthology_id": "K02355", "go_ids": [ "GO:0003746", "GO:0005525", "GO:0006414" ] }, "pscc": { "uniref50_id": "UniRef50_B5JXT8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B5JXT8" ], "product": "Elongation factor G" }, "genes": [], "id": "NKOCPLAKPK_506", "locus": "GCA003260355_00469" }, { "type": "cds", "sequence": "contig_1", "start": 243819, "stop": 244835, "strand": "-", "frame": 2, "gene": "fusA", "product": "elongation factor G", "db_xrefs": [ "GO:0003746", "GO:0005525", "GO:0006414", "KEGG:K02355", "SO:0001217", "UniRef:UniRef50_Q2NJ19", "UniRef:UniRef90_A0A7W4E4A3" ], "nt": "ATGGCAAACGACAAAAAACCTTTGGATAAGGTAAGGAATATCGGTATCATGGCGCATATTGATGCTGGTAAAACCACAACAACTGAGAGAATTTTGTATTACACAGGGAAGAAGCACAAGATTGGAGAAACTCACGATGGAGAAGCTGATATGGATTGGATGGAGCAGGAAAAAGAAAGAGGAATTACAATTACCTCTGCTGCTACGACATGTTTTTGGCACGACATCCAGGTAAATGTGATTGATACCCCAGGTCACGTAGATTTTACGGTAGAAGTAGAGAGATCTCTTAGAGTGCTTGATGGTGCTGTTGCTTTGCTTGATGTTTCTCAAGGAGCAGAACCTCAGACAGAAACGGTTTGGAGACAAGCTGATAAATATGGAGTTCCTAGACTCATCTTCGCAAATAAGATGGATAAAATGGGAGCAGACTTCTTCATGTCTCTTGAATCAGTAGAAAAAAGACTGAGCGATAAGGTAGTTCCTATTCAGCTCCCTATTGGAAAGGCTGATACTTTTGAGGGAATTATTGACCTTATTACAATGAAAGCCTATCATTTTGAAGGAGAAAAAGGAGTAGATGTTGTTGAAGGAGAAATCCCAGCCAATATGCAAGCACAAGCTGAAGAATGGAGAGAAAAAATGATTGATACAATCTCAATGTTTGATGATGAACTCGCTGAAAAATTCCTAGGAGGAGAAGAGATCAGTATCGATCTTATCAAAAAAGCAATTAGAACAGGAACTATTAGCAATCAGCTTTATCCTATGCTTTGTGGAACTGCACTTGGAAATAAAGGAGTTCAACTTGTACTCAATGCAGTAGTAGATTTCTTGCCTTCTCCACTTGATAGAGGGGAAATAGTTGGACATGATCCTGATAATGAAGAAAAAGTAGTAGTAAGGAGAGCCGACCCTAATGAGCCTGTTTCTGCTATTGCTTTCAAGATCATGACTGATCCTTTTGTAGGGACTTTGACTTTCGTGAGAGTATACTCAGGAACTATCAAGTCATGA", "aa": "MANDKKPLDKVRNIGIMAHIDAGKTTTTERILYYTGKKHKIGETHDGEADMDWMEQEKERGITITSAATTCFWHDIQVNVIDTPGHVDFTVEVERSLRVLDGAVALLDVSQGAEPQTETVWRQADKYGVPRLIFANKMDKMGADFFMSLESVEKRLSDKVVPIQLPIGKADTFEGIIDLITMKAYHFEGEKGVDVVEGEIPANMQAQAEEWREKMIDTISMFDDELAEKFLGGEEISIDLIKKAIRTGTISNQLYPMLCGTALGNKGVQLVLNAVVDFLPSPLDRGEIVGHDPDNEEKVVVRRADPNEPVSAIAFKIMTDPFVGTLTFVRVYSGTIKS", "aa_hexdigest": "a5e74004415e6ac826e933f2bad5ddf1", "start_type": "ATG", "rbs_motif": "TTTA", "psc": { "uniref90_id": "UniRef90_UPI00248C8BAB", "query_cov": 0.9792899408284024, "subject_cov": 0.8733509234828496, "identity": 0.628, "score": 416.0, "evalue": 1.82e-142, "valid": false, "product": "translation factor GTPase family protein", "uniref50_id": "UniRef50_A0A7X6X3H3", "kegg_orthology_id": "K02355" }, "genes": [ "fusA" ], "truncated": "5-prime", "pseudogene": { "start": 243519, "stop": 244835, "observations": { "mutation": [ 243821 ], "stop": [ 243821 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A7W4E4A3", "query_cov": 1.0, "subject_cov": 0.48216833095577744, "identity": 0.902, "score": 614.0, "evalue": 9.76e-216, "gene_start": 1, "gene_end": 338, "reference_start": 1, "reference_end": 338, "reference_sequence": "MANDKKPLDKVRNIGIMAHIDAGKTTTTERILYYTGKKHKIGETHDGAADMDWMEQEKERGITITSAATTCFWHDNQINVIDTPGHVDFTVEVERSLRVLDGAVALLDGSQGAEPQTETVWRQADKYGVPRLIFVNKMDKMGADFYMSIESVAQRLSNKAIPIQLPIGKADTFEGVIDLITMKAYHFEGEKGIDVVEGEIPADMQAQAEEYREAMIDAISMFDDELAEKFLGGEEISNDLIKRAIRKGVISNELYPMLCGTALGNKGVQLVLNAVIDFLPSPLDRGEMVGHNPDKEEETVVRKADINEPVSAIAFKIMTDPFVGTLTFVRVYSGVIKAGDTLLNSVTGKKERVGRLLLMHANKREEISEISAGNICAFLGLKETQTGHTLCDQNKPIVLEKMEFPEPVIHIAIEPKSKADQEKLGLALSKLMQEDPSFKAHSDEESGQTVIGGMGELHLDVIVDRLRREHKVEVNTGKPQVAYRETIFATGQARGIFKKQSGGRGQFGDVELRIEKLPEDKNYEFVSEIKGGVIPNEFIPAIDKGARETMAQGVLAGFPIINVKVVPVFGSYHDVDSSEVAFKVATYKAFREAFMKASPAILEPIMSVEIVTPEEYVGTVMGDLSSRRGIILGQTPRGNATAISAKVPLSEMFGYATDLRSNTQGRAAFSMEFSNYEKVPGMIEKKIIEERAGKVKKMDEE" }, "paralog": false, "identity": 0.910958904109589, "score": 803.0, "evalue": 1.73e-292, "description": "Internal stop codon at 243821. Nonsense mutation around 243821.", "psc": { "uniref90_id": "UniRef90_A0A7W4E4A3", "gene": "fusA", "product": "elongation factor G", "uniref50_id": "UniRef50_Q2NJ19", "kegg_orthology_id": "K02355", "go_ids": [ "GO:0003746", "GO:0005525", "GO:0006414" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A7W4E4A3", "UniRef:UniRef50_Q2NJ19", "KEGG:K02355", "GO:0003746", "GO:0005525", "GO:0006414" ] }, "pscc": { "uniref50_id": "UniRef50_Q2NJ19", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q2NJ19" ], "product": "Elongation factor G" } }, "id": "NKOCPLAKPK_507", "locus": "GCA003260355_00470" }, { "type": "cds", "sequence": "contig_1", "start": 245008, "stop": 245421, "strand": "-", "frame": 1, "gene": "terC", "product": "Tellurium resistance protein TerC", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0Q8N6U4", "UniRef:UniRef90_A0A328EG23" ], "nt": "ATGACTGATGAGTTTCACGGATCAGATTTCTTTGTGCTAAAAAACGGACTAAAATACGCCACTCCTCTATTACTCGTCGTAGGTGTGGTCGAGTTTTCAGATTTACTTTTTGCCGTAGATAGCATTCCTGCAATTTTTGCGATCGCTCCAAACGATCCCTTTATCCTCTACGCTTCCAACATTTTTGCAATTCTCGGATTAAGAAGCCTCTATTTTCTATTAGCGAACTTTATTGATAAATTCGCCTATTTAAAGTATGGAATAGCAGTAATTCTCACCTTTATTGGAATAAAAATGCTTCTGAGTCCTGTTATTCATTTCTCTTCTACTGCCTCACTGATTATCTTGATCAATATCCTCGTCCTCTCGCTAATCATTTCTCTGATTATGGAAAAGAAGAAAAAAAAGAGATAA", "aa": "MTDEFHGSDFFVLKNGLKYATPLLLVVGVVEFSDLLFAVDSIPAIFAIAPNDPFILYASNIFAILGLRSLYFLLANFIDKFAYLKYGIAVILTFIGIKMLLSPVIHFSSTASLIILINILVLSLIISLIMEKKKKKR", "aa_hexdigest": "c7f508f5bc707245f41d430b05d281c8", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A4R6QI05", "query_cov": 0.948905109489051, "subject_cov": 0.8783783783783784, "identity": 0.5, "score": 120.0, "evalue": 1.68e-32, "valid": false, "product": "TerC family integral membrane protein", "uniref50_id": "UniRef50_A0A3S0JM95" }, "genes": [ "terC" ], "truncated": "3-prime", "pseudogene": { "start": 245008, "stop": 245721, "observations": { "mutation": [ 245538, 245661 ], "stop": [ 245538, 245661 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EG23", "query_cov": 1.0, "subject_cov": 0.4228395061728395, "identity": 0.971, "score": 242.0, "evalue": 6.19e-78, "gene_start": 1, "gene_end": 137, "reference_start": 188, "reference_end": 324, "reference_sequence": "MYTHEMIVFGVIVSAMILLDLGLFNKKSHEVKTKEALIRTLVWIGLSMLFAGYVYRDSGVERFSQFLSAYWIEKLLSVDNLFVFVMIFSFFRVEKKDHHKVLFRGVIGALVFRAIFIFTGVKLIQLTYLPEFSIRNFSIKINILLSLFGIFLIYAGIKSFFQKEQAEEKQNFSDTLGAKIVKLIFPRMTDEFHESDFFVIKNGLKYATPLLLVVGVVEFSDILFAVDSIPAIFAIAPNDPFILYASNIFAILGLRSLYFLLANFIDKFAYLKYGIAVILTFIGLKMLLSPVIHFSSTASLIILINILVLSLIISLIMEKKKKKR" }, "paralog": false, "identity": 0.9662447257383966, "score": 435.0, "evalue": 2.13e-156, "description": "Internal stop codon at 245538, 245661. Nonsense mutation around 245538, 245661.", "psc": { "uniref90_id": "UniRef90_A0A328EG23", "product": "Tellurium resistance protein TerC", "uniref50_id": "UniRef50_A0A0Q8N6U4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EG23", "UniRef:UniRef50_A0A0Q8N6U4" ] }, "pscc": { "uniref50_id": "UniRef50_A0A0Q8N6U4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0Q8N6U4" ], "product": "Tellurium resistance protein TerC" } }, "id": "NKOCPLAKPK_508", "locus": "GCA003260355_00471" }, { "type": "cds", "sequence": "contig_1", "start": 245659, "stop": 245937, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATACTGCTTGACTTAGGACTCTTCAACAAAAAAAGTCACGAAGTGAAGACAAAAGAAGCACTTATACAAACACTTGTTTGGATCGGACTTTCAATGCTTTTCGCAGGATATGTCTATCGAGATTCGGGAGTAGAAAGATTTTCACAGTTCCTTTCTGCGTATTGGATAGAAAAATTACTCTCTGTAGATAATCTCTTTGTCTTTGTGATGATTTTTAGTTTTTTTCGTGTTGAGAAAAAGGCTCATCATAAAGTGCTATTTCGAGGAGTCATTTGA", "aa": "MILLDLGLFNKKSHEVKTKEALIQTLVWIGLSMLFAGYVYRDSGVERFSQFLSAYWIEKLLSVDNLFVFVMIFSFFRVEKKAHHKVLFRGVI", "aa_hexdigest": "db4bc68f6f34b43f4dce9c9444f09e74", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10804.736999999994, "isoelectric_point": 9.571766090393066 }, "id": "NKOCPLAKPK_509", "locus": "GCA003260355_00472" }, { "type": "cds", "sequence": "contig_1", "start": 246182, "stop": 246301, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGTCTTTATTTTTTTATAAAAATACACCCATGAAAAAACTAGTAAAAAAACTCCACAAAGCTGAGGCTGAAAGAACAAAGGTTCAGAAGATTGTTAGAGATGTAGCTTTCTTTTGA", "aa": "MLSLFFYKNTPMKKLVKKLHKAEAERTKVQKIVRDVAFF", "aa_hexdigest": "2f4c02b1af3786eda514c6a3dbae5ca0", "start_type": "ATG", "rbs_motif": "TTATT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4684.658, "isoelectric_point": 10.29284496307373 }, "id": "NKOCPLAKPK_510", "locus": "GCA003260355_00473" }, { "type": "cds", "sequence": "contig_1", "start": 246323, "stop": 246424, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7W4E2Y9", "UniRef:UniRef90_A0A660MZ83" ], "nt": "ATGACATTTATGGTCTTTACTGCAGAAGCAGATAAAACTGAGGCTGAACTTGGTGTTGTATCAACAACTATTGAAGCTTCACACGTTGCTCTTGCAAGATAA", "aa": "MTFMVFTAEADKTEAELGVVSTTIEASHVALAR", "aa_hexdigest": "b1f9391f78e821da15e751a525e42b39", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 246215, "stop": 246424, "observations": { "mutation": [ 246299 ], "stop": [ 246299 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A660MZ83", "query_cov": 1.0, "subject_cov": 0.4782608695652174, "identity": 1.0, "score": 61.2, "evalue": 1.1e-11, "gene_start": 1, "gene_end": 33, "reference_start": 37, "reference_end": 69, "reference_sequence": "MKKLVKKLHKAEAERTKVQKIVRDVAFFGVVVYAAVMTFMVFTAEADKTEAELGVVSTTIEASHVALAR" }, "paralog": false, "identity": 0.9855072463768116, "score": 127.0, "evalue": 1.75e-40, "description": "Internal stop codon at 246299. Nonsense mutation around 246299.", "psc": { "uniref90_id": "UniRef90_A0A660MZ83", "uniref50_id": "UniRef50_A0A7W4E2Y9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A660MZ83", "UniRef:UniRef50_A0A7W4E2Y9" ] }, "pscc": { "uniref50_id": "UniRef50_A0A7W4E2Y9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7W4E2Y9" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 3526.985199999999, "isoelectric_point": 4.571128273010253 }, "id": "NKOCPLAKPK_511", "locus": "GCA003260355_00474" }, { "type": "cds", "sequence": "contig_1", "start": 246485, "stop": 246841, "strand": "-", "frame": 3, "gene": null, "product": "Cation efflux protein cytoplasmic domain-containing protein", "db_xrefs": [ "KEGG:K13283", "SO:0001217", "UniRef:UniRef50_A0A328EHY5", "UniRef:UniRef90_A0A328EHY5" ], "nt": "ATGGATCCAATTATTGCGAGTGGAATGGCACTTTACATGATCTATAGTGCACGAAGCATTGCAAAAAAAGGAATTGACCTCTTAATGGACACAAGTTTAGGTAAAGACCATGAAATTGGAACAATCATTCTAGCCCATCCTGCCGTAAAAAGCTTCCACGAACTCAGGACCCACCAATCAGGAAACCGAGCATTTATCAGCTTTCATATGGTGTTTAAAAACGCGGGGATTAGCCTCAAAGAAGCCCACCAAGTAAACAATGACGTCGAAACTTCGATCAAAAAGAAATTTAAAAAAGCAAATGTACTCATCAAACTAGATCCAATTGACGACGAAAAAAGCCTGCAACAATATTAA", "aa": "MDPIIASGMALYMIYSARSIAKKGIDLLMDTSLGKDHEIGTIILAHPAVKSFHELRTHQSGNRAFISFHMVFKNAGISLKEAHQVNNDVETSIKKKFKKANVLIKLDPIDDEKSLQQY", "aa_hexdigest": "1130709bfa1b9411bd2f4e7e618560ec", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 246485, "stop": 247141, "observations": { "mutation": [ 246889, 247027, 247033, 247105 ], "stop": [ 246889, 247027, 247033, 247105 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EHY5", "query_cov": 1.0, "subject_cov": 0.4054982817869416, "identity": 0.975, "score": 223.0, "evalue": 1.95e-71, "gene_start": 1, "gene_end": 118, "reference_start": 174, "reference_end": 291, "reference_sequence": "MLSAKKVIRIASLGAALLAVIKLTLGIISGSMVIIASAVDSVMDLIISMLNLFALKAAEKPADKEHNYGHGKIEAIVALAESVFIGGSGIILIYSSIQKYLNRTPLEETGAGIIAMGIAILITGIIIFLLRKVNKKDKSLIIQADLMHYEMDILTNIGIIITLIIIQHTQWFWMDPIIAGGMALYMIYSARSIAKKGIDLLMDTSLGKDHEIGAIILAHPAVKSFHELRTHQSGNRAFISFHMVFKNAGISLKEAYQVNNDVETSIKKKFKKANVLIKLDPIDDEKSLQQY" }, "paralog": false, "identity": 0.9587155963302753, "score": 400.0, "evalue": 3.13e-143, "description": "Internal stop codon at 246889, 247027, 247033, 247105. Nonsense mutation around 246889, 247027, 247033, 247105.", "psc": { "uniref90_id": "UniRef90_A0A328EHY5", "product": "Cation efflux protein cytoplasmic domain-containing protein", "uniref50_id": "UniRef50_A0A328EHY5", "kegg_orthology_id": "K13283", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EHY5", "UniRef:UniRef50_A0A328EHY5", "KEGG:K13283" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EHY5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EHY5" ], "product": "Cation efflux protein cytoplasmic domain-containing protein" } }, "id": "NKOCPLAKPK_512", "locus": "GCA003260355_00475" }, { "type": "cds", "sequence": "contig_1", "start": 246887, "stop": 247015, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGAATTGCGATTTTCATTACTGGAATCATCATTTTTTTACTCAGAAAGGTAAACAAAAAGGATAAAAGTCTGATTATTCAGGCAGATTTAATGCACTATGAGATGGATATCCTTACCAATATTTGA", "aa": "MGIAIFITGIIIFLLRKVNKKDKSLIIQADLMHYEMDILTNI", "aa_hexdigest": "1045fb2a03a6d2b824f370eb4d1837aa", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4847.8874, "isoelectric_point": 8.1610689163208 }, "id": "NKOCPLAKPK_513", "locus": "GCA003260355_00476" }, { "type": "cds", "sequence": "contig_1", "start": 247103, "stop": 247267, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTAATCATCGCATCTGCAGTAGATTCCGTAATGGACCTAATCATCTCAATGCTCAATCTCTTCGCGTTAAAAGCAGCAGAAAAACCAGCAGATAAAGAACACAATTACGGACACGGAAAAATCGAAGCAATCGTAGCCCTTGCAGAAAGCGTTTTCATCTGA", "aa": "MVIIASAVDSVMDLIISMLNLFALKAAEKPADKEHNYGHGKIEAIVALAESVFI", "aa_hexdigest": "8a2302ff161a0f21185dc21b69e9feb9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5798.789599999998, "isoelectric_point": 5.006287574768066 }, "id": "NKOCPLAKPK_514", "locus": "GCA003260355_00477" }, { "type": "cds", "sequence": "contig_1", "start": 247939, "stop": 248256, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08903.17" ], "nt": "ATGGGAAGTAGTTTTGATAACAAAAAATATCTTGAATCCGCAATCTCAAACCTCAAAGAGAAAGTAACAAAAACCAGAGGAAAACTCTACGTTGAAATCGGTGAGGAATTACTCAAAAACAATTCTACCACAACAGAAATTCCAGGTTTCGATCTAGATAACAAGAAAAAAATAGTTTCAGAATTCAGACAGGATTCTGAGATTTTAGTCTGTATCAATGCACGAAATATCATTGAAAATCAGCCTCTAGTCGAGAACGGACTTCCATGTATTGATCATATTGAACTAATGTTAAAAAAAATCTCCACTGCAACCTGA", "aa": "MGSSFDNKKYLESAISNLKEKVTKTRGKLYVEIGEELLKNNSTTTEIPGFDLDNKKKIVSEFRQDSEILVCINARNIIENQPLVENGLPCIDHIELMLKKISTAT", "aa_hexdigest": "460659e327781a40369c3fdf5fe21289", "start_type": "ATG", "rbs_motif": "TTTA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08903.17", "name": "DUF1846", "length": 78, "aa_cov": 0.7428571428571429, "hmm_cov": 1.0, "evalue": 4.085741144171644e-17, "score": 58.406341552734375, "start": 4, "stop": 81 } ], "seq_stats": { "molecular_weight": 11856.501399999986, "isoelectric_point": 5.855405235290527 }, "id": "NKOCPLAKPK_515", "locus": "GCA003260355_00478" }, { "type": "cds", "sequence": "contig_1", "start": 248548, "stop": 249021, "strand": "-", "frame": 1, "gene": null, "product": "Ferric uptake regulation protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBFF2EC", "UniRef:UniRef100_A0A328EJ93", "UniRef:UniRef50_A0A328EJ93", "UniRef:UniRef90_A0A328EJ93" ], "nt": "GTGAAAGGGGTTTACAAGAGAAAGTTAAAAAATCTGATAAGAAATATCAAGCTCATCTTGATTTCGAGTAACCAAAAATATATGATACCAAACATTCTGCTCAATAAACTACAAACAATGCCAAAAGTTTTTAAATACAAAAAAATCATCCTCAAAGTCTGTGATGGTCAACATCGCCACACAGAACAGATTTTTTTTGAACTTAAAAAATATTTTCCCAATATCTCACTCTCCACGGTCTATAGAAACATCAATGAACTCATAGACTCAGGAGAGCTCAAAGAAGCAATAGTTTTAGGAGGAAGAAGCTATTATGAATCCACCGCAAAAGAACACGCCCATCTTATTGATACTGAATCTGGAGAAATCTACGACATTGACCTTCCGGTAGTAAAAGTGGAAGGGATTCCTGCTGGATTTTCAGCCCAAAAAATTAGTCTAAATATCTACGGAACATTCAAAAAAAGCCCTTAA", "aa": "MKGVYKRKLKNLIRNIKLILISSNQKYMIPNILLNKLQTMPKVFKYKKIILKVCDGQHRHTEQIFFELKKYFPNISLSTVYRNINELIDSGELKEAIVLGGRSYYESTAKEHAHLIDTESGEIYDIDLPVVKVEGIPAGFSAQKISLNIYGTFKKSP", "aa_hexdigest": "ca774ba63674e23a2bd67b73cf526d74", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000DBFF2EC", "uniref100_id": "UniRef100_A0A328EJ93", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBFF2EC", "UniRef:UniRef100_A0A328EJ93" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EJ93", "uniref90_id": "UniRef90_A0A328EJ93" }, "psc": { "uniref90_id": "UniRef90_A0A328EJ93", "product": "Ferric uptake regulation protein", "uniref50_id": "UniRef50_A0A328EJ93" }, "pscc": { "uniref50_id": "UniRef50_A0A328EJ93", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EJ93" ], "product": "Ferric uptake regulation protein" }, "genes": [], "id": "NKOCPLAKPK_516", "locus": "GCA003260355_00479" }, { "type": "cds", "sequence": "contig_1", "start": 249065, "stop": 249346, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00133.28" ], "nt": "ATGGCGACAAATCTAGAGCGTATGAATGAAATCCTTGATTTCTGGATGAAAGATAAGACTTTCCAGAGATCTATTGATGAAAGAGCAGAGGCAATGAGCTATAAATTCTTTGATGGTCCTCCTTTTGCGAGTGGAAGTCCACACTATGGGCATCTTTTGGCTGGAAGTATCAAGGATGTGATCCCAAGGTATAGAACCATGAGAGGGTATAAAGTAAAGAGAAAGCGAGGTTGGGACTGTCATGGTCTCCCTGTAGAAAAAGCAGTAGAGAAGGCACTTTGA", "aa": "MATNLERMNEILDFWMKDKTFQRSIDERAEAMSYKFFDGPPFASGSPHYGHLLAGSIKDVIPRYRTMRGYKVKRKRGWDCHGLPVEKAVEKAL", "aa_hexdigest": "600fb35b33afcbac8eb514067b0dc4d9", "start_type": "ATG", "rbs_motif": "TTTTT", "psc": { "uniref90_id": "UniRef90_A0A2S5D0S6", "query_cov": 0.956989247311828, "subject_cov": 0.9081632653061225, "identity": 0.528, "score": 106.0, "evalue": 3.08e-28, "valid": false, "product": "Isoleucine--tRNA ligase", "uniref50_id": "UniRef50_A0A2S5D0S6" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00133.28", "name": "tRNA-synt_1", "length": 83, "aa_cov": 0.8924731182795699, "hmm_cov": 1.0, "evalue": 5.068742905875205e-30, "score": 100.96235656738281, "start": 11, "stop": 93 } ], "seq_stats": { "molecular_weight": 10823.395399999992, "isoelectric_point": 9.516000938415527 }, "id": "NKOCPLAKPK_517", "locus": "GCA003260355_00480" }, { "type": "cds", "sequence": "contig_1", "start": 249416, "stop": 253222, "strand": "+", "frame": 2, "gene": "ileS", "product": "isoleucine--tRNA ligase", "db_xrefs": [ "EC:6.1.1.5", "GO:0000166", "GO:0004822", "GO:0005524", "GO:0006428", "SO:0001217", "UniRef:UniRef50_A0A328EJJ5", "UniRef:UniRef90_A0A328EJJ5" ], "nt": "GTGAATCAAACGAACGCTGATTGGAAGCGGTTTGTGGATCATACTGGACGCTGGGCTGATATTGAGAATCCGTATTTCACGATGGATTTGGATTTTATGGAAAGCGTGATCTGGTGTTTCTCTAGCATTTACAATCAAAATAAGGTCTATAAAGGGTTTAAGGTGCAGGGATACTGCCCAAGCTGTGCAACTCCGCTTGCCAACAACGAAATCTCTGATGGCTATGAAGACAAGCAAGATATGGCAATCACTATCAAGTTTGCTCATCAAGCTCCAAAAAGTAGTGCATACGAAGCTTCTGAAGATGGTTTTATTGATGTAGTAGCAGGAGTGATCAGAGATGGCGAGGGTCGCTATGCGATGATTCATCACATGAAGGAGAATCTCTGGTTTTTCCCTGGTGGAAAAGTTGAAAAAGGAGAATGCCGAATCGCAGCACTTAAGAGAGAACTCAAAGAAGAAATCGGAGTTGAAGCTGAAGAAAAACACTATCTCGGAGCGATCAAAATCATTCATCTCGGGAAACCTTATAGAGTCCATTGGTTTGAGGTAACAACCAGCGATACGCCAAGCGTTCAGGAAAGCGAAAAGCACAATGCACTCGTTTGGGTAAAAGAAGAAAAGTCTGAAAATAGCCTCGGATTTGCCCTTAATATCAACGGAACAATTATTGATGATGAAAAGGAACTTACGCATGATTTCATTGATTTTCATCTTTTCAAAAAAGTTTTGGATCCTGAGGATAAAGGCTTGATTGAGGGGAAATTCAATTTCCTTGCTTGGACGACGACGCCTTGGACTTTGCCGAGCAATATGTTTCTCGCGGTCGGAGCTGAGATTGACTATGTGACCGTTTACGATCCTGAGAGCCAAGAATACTATATTATGGCGGAAAATCTGTTGAAAAAATACTACAAATCCCCAGACCAATACAAATTTATCTACAAGCAAAAAGGAAAAGAGCTTAAAAATCTGACCTATCAACCGCTCTTTGACTATATTCCAAATTCCAAGATTCCAACTGAATACAAGAGTCAGTTTTTCCAAATTTTGAATGGAGATTTCGTAAGTACTGAGGATGGAACTGGAATCGTGCATATCGCACCGACCTTTGGAGAAATAGACTTTATGGCGGTAGCGGAACTCCTCGGTGAACAGCATGCTTTGGAGTGGCTCTTTATGCCAGTTGATGAGTATGGTTGCTTTGATGAGCAAGTGCCTGACTATCAAGGGATGAAGGTCTTTGATGCCAATAAGCTCGTGATGGATGAACTCAAGGCGAGAAAAGTGACGGTGAAAGCGGAATCTATAACCCACTCCTATCCTCACTGTCGAAGATGCCATTCTCCATTGATCTACAAGGCAATGTCAAGTTGGTTTATCAAGGAGAAAGAAATGAATGCTCAGACCTACCAACAAGCCGAGCAGATCAATTTCGTGCCTGAGACCGTAAAAAACAGATTCGTGAATGGACTTCAGCAGGCTCCTGACTGGAATATCGCGAGAAATAGATACCGAGGTTCTCCTTTGCCGATTTGGCAGAATGTGGAAGATCCTGAAGATCGCTTCTCGCTTGGGACGCTAGATGAGCTTTATCATCTCACCAAAACAGGATCAAAAAATCTGACCAAAAATCTCTTTATCCGCCACGGAAGGACAGATTTTAACGATGAGAAAAAGTTTGATGGACTTGGGGTTTCTGTCTTGACTCCAGAAGGACAAACGCAAGCCAACGCACTTGTTGAGAAGCTTGCAAAGCTAAAAGAAGAAAAAGCTGATCTCGTTTTCGTCGTTTCTCCTCTTCCAAGAGCGTGGGAAACCCTGAAACCGAGTCTGATTTCTTTCTTTGATGAAACGCTTGTTGCTGACTGTGAAAAGAAATACTTTGAAATCGTAAAGAGCTATCAAGAAGCTTTCAATAAGGGAACTTTGCTTGAAATCGTGCAGGGAAGCGATGAGCAAAATGTTTTCCAGCTTCACGAACAGATTTTCGTGGATTGGAGGATGACGGATCACCTTTCTTTTGCAGACCAAGGGAAAGTTCAGCCTTGTGATCTTTTGAACTGGCAAGATGCAAGCAAATCGATCGGAACTGATGGTGAAACGAACGAAACTATGTTTGTGAGAGTAAAAAATGCGGTGCAGTATTGGAATGCAAAGTTTGCGACAAAAACGCTTATCTACGCTTCTCACAACGATACGATTGCGATGGCGAGAAAAGCTTTCCGCAACTTTGATTATGCGAGCAAGAGAAAGCAGTTCTTGACTAAAAATGGAGAAATTCAGACGCATTACCGAGATAATGCGAGAAATACCGAAGTGGATCTTCATAAGCCGTATGTGGATTCTTACTGGGGAATGAGAGAAGGTAAGACCTACAAGAGAATCCCTGAAGTAATGGATTGCTGGTTTGAATCAGGTTCTATGCCGTTTGGACAGGATCATTATTTGGGGGAAGAAAAAGCTGAACTCACCTATCCAGCCGACTTTATCGCTGAAGGACTAGATCAAACCAGAGGTTGGTTCCGTTCTCTCCATGTCGTAGGACACGCAATCAAAGGAGAAAATGCCTTCAAAAATGTGATCGTAAACGGACTAATTCTCGCAGAAGATGGAAAGAAAATGGCGAAAAGTCTGAAGAATTATCCAGATCCTAGAGGACTCATTGAAAAACGAGGAGGGGATGCTTTCAGACTCTATACTTTGAGTGCTCCTGTTGTAAGAGCTGAACCGATGAGATTCGCTGAAAAAGGAGTAGAACAATGCTTCAAAGATTTCAATATTCCACTAGAAAATGTCTTCAAGTTCTTTGAAACCTACGCAAAGATTGATAAGCGAAAGAGTGATGGAACTGAGGTTTTCTTGATGAGGCATGCAAAAGCCGAAGGGCAGGGCATGAATGATCTACTTACTGCAGAAGGGAAAGCTGAACTTGAAAGTCAGGAAACGAGAGAAAAAATCATCAGACTTAATCCTGATATTATCGTTCATACACCATTTTTGAGAACGAAAACTACTGCCGAATATGTGCAGCAAACCCTCAAAACCCTTACAGGAAGAGAAATACCGCTTCAAGTCATCAATCCACTTGGTGATGACAACGCAAAAAAATCCTATATGAAAGCCCTCTATGATGATCTCTTGAAAGAACATCAAGGGAAAAGAATCTTGATCATAGGTCATGCCTATACTTTGAGAACGATCCGAGAAATTCTTTATGAAAACTATTTTGAAGATTATGCTTATACCGAAGTTCAAAGACAAGAAGGACTCAAGCAGCTTGAAATCGTTAAACTTCCGACCTATCAGATTGCAAATGAGCTTGATAAGTGGATTCTCGCAGAGCTTCATCAGACTTTGAAGCTTGTAGATCACTACCTTGAGGCTTGTGAACTTGATGCTGCTCTCAAAAGCGGTATGGAATTCGTTGAGAAGCTTACCAACTGGTATCTTAGAAGAAGCAGGAGAAGATTCCGAGGAAGTGAGATGACAACTGATAAACATTCAGCATACGCTACTCTCTTTGAGGTCTTGAGGACCTATCTCCAGATGATGGCTCCATTTACGCCGTTCATCACTGAGGAACTCTGGCAGAAACTTCAGACTTTTGTTGCTGGTAAAGAAGGAGAAAAAGCTGAATCGCTCCACCTTGCTTATTGGCCATTCGCGAGTGAAAAATACATTGATCAGCAGCTTATGGAAGAAATTGCAACCGTGAGAAAAGCGATCAAGCTTGCCCTCTTCATCAGATCAAAGAATAAAATCGCGGTTAAGCAACCTCTGCAAAAGCTGAGCATTAAGCTGTAA", "aa": "MNQTNADWKRFVDHTGRWADIENPYFTMDLDFMESVIWCFSSIYNQNKVYKGFKVQGYCPSCATPLANNEISDGYEDKQDMAITIKFAHQAPKSSAYEASEDGFIDVVAGVIRDGEGRYAMIHHMKENLWFFPGGKVEKGECRIAALKRELKEEIGVEAEEKHYLGAIKIIHLGKPYRVHWFEVTTSDTPSVQESEKHNALVWVKEEKSENSLGFALNINGTIIDDEKELTHDFIDFHLFKKVLDPEDKGLIEGKFNFLAWTTTPWTLPSNMFLAVGAEIDYVTVYDPESQEYYIMAENLLKKYYKSPDQYKFIYKQKGKELKNLTYQPLFDYIPNSKIPTEYKSQFFQILNGDFVSTEDGTGIVHIAPTFGEIDFMAVAELLGEQHALEWLFMPVDEYGCFDEQVPDYQGMKVFDANKLVMDELKARKVTVKAESITHSYPHCRRCHSPLIYKAMSSWFIKEKEMNAQTYQQAEQINFVPETVKNRFVNGLQQAPDWNIARNRYRGSPLPIWQNVEDPEDRFSLGTLDELYHLTKTGSKNLTKNLFIRHGRTDFNDEKKFDGLGVSVLTPEGQTQANALVEKLAKLKEEKADLVFVVSPLPRAWETLKPSLISFFDETLVADCEKKYFEIVKSYQEAFNKGTLLEIVQGSDEQNVFQLHEQIFVDWRMTDHLSFADQGKVQPCDLLNWQDASKSIGTDGETNETMFVRVKNAVQYWNAKFATKTLIYASHNDTIAMARKAFRNFDYASKRKQFLTKNGEIQTHYRDNARNTEVDLHKPYVDSYWGMREGKTYKRIPEVMDCWFESGSMPFGQDHYLGEEKAELTYPADFIAEGLDQTRGWFRSLHVVGHAIKGENAFKNVIVNGLILAEDGKKMAKSLKNYPDPRGLIEKRGGDAFRLYTLSAPVVRAEPMRFAEKGVEQCFKDFNIPLENVFKFFETYAKIDKRKSDGTEVFLMRHAKAEGQGMNDLLTAEGKAELESQETREKIIRLNPDIIVHTPFLRTKTTAEYVQQTLKTLTGREIPLQVINPLGDDNAKKSYMKALYDDLLKEHQGKRILIIGHAYTLRTIREILYENYFEDYAYTEVQRQEGLKQLEIVKLPTYQIANELDKWILAELHQTLKLVDHYLEACELDAALKSGMEFVEKLTNWYLRRSRRRFRGSEMTTDKHSAYATLFEVLRTYLQMMAPFTPFITEELWQKLQTFVAGKEGEKAESLHLAYWPFASEKYIDQQLMEEIATVRKAIKLALFIRSKNKIAVKQPLQKLSIKL", "aa_hexdigest": "4bbdd5348d281acd024b309b98a5c731", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A328EJJ5", "query_cov": 1.0, "subject_cov": 0.9155234657039711, "identity": 0.9990000000000001, "score": 2569.0, "evalue": 0.0, "valid": true, "gene": "ileS", "product": "isoleucine--tRNA ligase", "ec_ids": [ "6.1.1.5" ], "uniref50_id": "UniRef50_A0A328EJJ5", "go_ids": [ "GO:0000166", "GO:0004822", "GO:0005524", "GO:0006428" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EJJ5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EJJ5" ], "product": "Nudix hydrolase domain-containing protein" }, "genes": [ "ileS" ], "id": "NKOCPLAKPK_518", "locus": "GCA003260355_00481" }, { "type": "cds", "sequence": "contig_1", "start": 253315, "stop": 253776, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EG71", "UniRef:UniRef90_A0A328EG71" ], "nt": "GTGAGCTCAGCTTACTTTCCTTATAAAAGTGAGTGGAATTGTAGTAAAATTGAGGATAAAGAAGCTAGAGAAATGTGCGAAAACGAAATGTCTTTTGCCAAATTTACCGAAACAACAGGGACAAGAGAAATGTCTACCACTTGCTGTAGCTATTTAGACCATGATCCTAAGTTCAACCTACGGCGAGAACAGGTAACAGCATTTGGAGGACTTGATAAACGGACAAACGAAGCTTCTGAAGAAATAAAAAATAAACCCGATCAGGATCGAGCCCTTATCTCAGGTTCTGTCCAACAACTCACAGATCTTATTCTTAAGTTAAGCACTCAGATTTACGAAGAAGCTTCAAATAAAATTCAGGCTACTAAAATGCTATCTTCGCTCTTCAAAGGTTGTAGTGAAAATAATAAGAACCTTGAAGCGAGAATCGTTTGTAAATACTTCGCCTATGATCTTATTTGA", "aa": "MSSAYFPYKSEWNCSKIEDKEAREMCENEMSFAKFTETTGTREMSTTCCSYLDHDPKFNLRREQVTAFGGLDKRTNEASEEIKNKPDQDRALISGSVQQLTDLILKLSTQIYEEASNKIQATKMLSSLFKGCSENNKNLEARIVCKYFAYDLI", "aa_hexdigest": "3257312169ad414198c159517c5514f9", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 253261, "stop": 254079, "observations": { "mutation": [ 253273, 253282, 253312, 253774, 253951, 254026 ], "stop": [ 253273, 253282, 253312, 253774, 253951, 254026 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EG71", "query_cov": 1.0, "subject_cov": 0.4273743016759777, "identity": 0.993, "score": 304.0, "evalue": 9.4e-102, "gene_start": 1, "gene_end": 153, "reference_start": 19, "reference_end": 171, "reference_sequence": "MKRNGILGIIISLSLICGVSSAYFPYKSEWNCSKIEDKEAREMCENEMSFAKFTETTGTREMSTTCCSYLDHDPKFNLRREQVTAFGGLDKRTNEASEEIKNKPDQDRALISGSVQQLTDLILKLSTQIYEEASNKIQATKMLSSLFKGCSENNKNLEARIVCKYFAYDLIGKEPKITPISLSDITTAFQKEKKLIQRSVQFSDLNENYTPINYHYATIRKMYRIGGFAFGLMDSYDYFDKDSDTDFFKILVLKAGDTHWQDFADIKVNANFTDELALTFESHKLGLTIPVLKNKEKENERLTAQFQMQRDGVRKLVGCFEEKFNGTQATTEDLISRKKLPLKSCEALGMEVTTTLGK" }, "paralog": false, "identity": 0.9779411764705882, "score": 528.0, "evalue": 4.15e-192, "description": "Internal stop codon at 253273, 253282, 253312, 253774, 253951, 254026. Nonsense mutation around 253273, 253282, 253312, 253774, 253951, 254026.", "psc": { "uniref90_id": "UniRef90_A0A328EG71", "uniref50_id": "UniRef50_A0A328EG71", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EG71", "UniRef:UniRef50_A0A328EG71" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EG71", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EG71" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 17561.620099999996, "isoelectric_point": 5.344536399841308 }, "id": "NKOCPLAKPK_519", "locus": "GCA003260355_00482" }, { "type": "cds", "sequence": "contig_1", "start": 254849, "stop": 255664, "strand": "+", "frame": 2, "gene": null, "product": "Peptidase metallopeptidase domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MVY3", "UniRef:UniRef90_A0A660MVY3" ], "nt": "ATGTATGTATTGATTATCGGCGTTTTCTCTGTTTCGTGTGATAAGAATGCAGAACTTCTAAGTTCTGACCCAATAGAACATTTTGTTCTGCATCAGGAATCCTCCTTTGATATTCAAAGGAAAATTGATTATCTGTGTTCCATGTATGGTTTTAAGGAGTCTTCTGTCCAACTTCGAGATAGTCTGTTAATGGTAGAGGGAGATATAGCTTTTCATTTAAACGGTTTTTGGGAGAAATATGCTGATCGATATGAAGATCAGGTACAGTTGAGATCCCATTATGCTCGCAGAGGGCTTGTTGCATCTCAGTATAGGACAATTCTTCTGAGTGTTCCTTCGGCAGGAGATCGATATGCACTACCTACAAGCTGGCTTACCGCTTTGATTTATGCTGCTGAAGCATGGAATGGTCTTAATGGCCCTATCCGGTTCACTTTTTATAGGGGAGATCCGTATACATTTACTGTAAATGTTATGTATGTCTTTGGTAGTGATCCTGAAACTTTAACTTGGGCTGATTATCCTGGAGGTGGAAAACCTGGGAGTTATGTTTCTATTAATTCTAATGGTTTTGATGGATCTACAAGACCTCTCAATAGTAGAACTGCTGTTTTGATTCATGAATTGGGACACGTGATTGGTTTTTCGCATACAGATGAGGTTCCGGCCAATGGTTATTCTTATCTTTTAGATACTGGAGATAATAATCTTGAGGATCCAACTTCTGTCATGTGGCATCATGCACTGTCAAATTCGCAACCGCCTTATTTTTCTGCTTACGATATAGAAGCTTACAGATATTTGTATGGCTTTTAG", "aa": "MYVLIIGVFSVSCDKNAELLSSDPIEHFVLHQESSFDIQRKIDYLCSMYGFKESSVQLRDSLLMVEGDIAFHLNGFWEKYADRYEDQVQLRSHYARRGLVASQYRTILLSVPSAGDRYALPTSWLTALIYAAEAWNGLNGPIRFTFYRGDPYTFTVNVMYVFGSDPETLTWADYPGGGKPGSYVSINSNGFDGSTRPLNSRTAVLIHELGHVIGFSHTDEVPANGYSYLLDTGDNNLEDPTSVMWHHALSNSQPPYFSAYDIEAYRYLYGF", "aa_hexdigest": "2608e5b441255fd49b2a4d0cac78ca3a", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A660MVY3", "query_cov": 1.0, "subject_cov": 0.9748201438848921, "identity": 0.948, "score": 529.0, "evalue": 9.04e-190, "valid": true, "product": "Peptidase metallopeptidase domain-containing protein", "uniref50_id": "UniRef50_A0A660MVY3" }, "pscc": { "uniref50_id": "UniRef50_A0A660MVY3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MVY3" ], "product": "Peptidase metallopeptidase domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_520", "locus": "GCA003260355_00483" }, { "type": "cds", "sequence": "contig_1", "start": 255823, "stop": 256089, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGCTAGTAATGATATTAAAGAGTATTTAGAAGATCAAATTTTGTCTCATCACTCTATTAATATTCCAGTTTGCAATGATGCTCAAAATGTTCACAGGTTGTTTTCTACCTTGAAGCAGGGTGATTTCTTTGATGTTGAAAATTATGGAAATAAAAAATATTTTAATATTCTTTTGGATTTTGTGCTTAACAATCATCCAACAAATTTATTAATGAGATCTTTATTTCCGTATCTTGATTCCAAATATATAGAGAATGGGGAATGA", "aa": "MASNDIKEYLEDQILSHHSINIPVCNDAQNVHRLFSTLKQGDFFDVENYGNKKYFNILLDFVLNNHPTNLLMRSLFPYLDSKYIENGE", "aa_hexdigest": "b0586fb41358d987262788a4a7fb663c", "start_type": "TTG", "rbs_motif": "TTGTA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10324.477199999992, "isoelectric_point": 5.174303627014159 }, "id": "NKOCPLAKPK_521", "locus": "GCA003260355_00484" }, { "type": "cds", "sequence": "contig_1", "start": 256270, "stop": 256581, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTTAAAAAATTGTCCTATAATAAGGATGGAGTATTGTTTCTTGGATGTTCTACTAATTATCAATCTCAATACATTACTCCGCACTTCGCAAGAATTAGAAGAAGGTTAGGAGATAAAAACTATAGTTATTATGTTGATGACCGTTTTTATGTTAAAACTCCTTATTTAGAGCTTACACAATATGATGGGAAAGATATTACCCTTAGATTCTATGTGGATCCATTTTCTTTTCAATCAAATTCTAACTTAGAATGCGAGAATTCACGAATGTTAAAATATGAGATTGTATCGGAAAATGAGAAAGCATAA", "aa": "MVKKLSYNKDGVLFLGCSTNYQSQYITPHFARIRRRLGDKNYSYYVDDRFYVKTPYLELTQYDGKDITLRFYVDPFSFQSNSNLECENSRMLKYEIVSENEKA", "aa_hexdigest": "981a5a08295a5c1c7f41cd0c11512454", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12328.746099999991, "isoelectric_point": 8.486054039001466 }, "id": "NKOCPLAKPK_522", "locus": "GCA003260355_00485" }, { "type": "cds", "sequence": "contig_1", "start": 256765, "stop": 256974, "strand": "+", "frame": 1, "gene": null, "product": "HU domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EJJ9", "UniRef:UniRef90_A0A328EJJ9" ], "nt": "TTGGCAGATATTCCTAGACTTCTAGTGGCTTTATTTTCTGACCAACTATTCACTAAGGAAACTGCGACTGTAGATTCTACCGTTTCTGATCCTGATGTTCTCGCAGTGTTTAGTGATCTTACGAAAGTATTAGCAAAACATTTAGTAGAAGGAAGGATTTTAAAGTTTGGAGATTTTGGAAACTTTCAGCTAACACTCAAAAGCGAATGA", "aa": "MADIPRLLVALFSDQLFTKETATVDSTVSDPDVLAVFSDLTKVLAKHLVEGRILKFGDFGNFQLTLKSE", "aa_hexdigest": "7e612b71679a9f2eb98b61de1031c3ac", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 256765, "stop": 257094, "observations": { "mutation": [ 256972, 257038 ], "stop": [ 256972, 257038 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EJJ9", "query_cov": 1.0, "subject_cov": 0.6330275229357798, "identity": 1.0, "score": 134.0, "evalue": 1.21e-39, "gene_start": 1, "gene_end": 69, "reference_start": 1, "reference_end": 69, "reference_sequence": "MADIPRLLVALFSDQLFTKETATVDSTVSDPDVLAVFSDLTKVLAKHLVEGRILKFGDFGNFQLTLKSEGVESAEKFIASHIIGNKIQFCPGTYLRDMLAMLKYEKYTK" }, "paralog": false, "identity": 0.9724770642201835, "score": 206.0, "evalue": 1.84e-70, "description": "Internal stop codon at 256972, 257038. Nonsense mutation around 256972, 257038.", "psc": { "uniref90_id": "UniRef90_A0A328EJJ9", "product": "HU domain-containing protein", "uniref50_id": "UniRef50_A0A328EJJ9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EJJ9", "UniRef:UniRef50_A0A328EJJ9" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EJJ9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EJJ9" ], "product": "HU domain-containing protein" } }, "id": "NKOCPLAKPK_523", "locus": "GCA003260355_00486" }, { "type": "cds", "sequence": "contig_1", "start": 257189, "stop": 257902, "strand": "+", "frame": 2, "gene": "sufC", "product": "Fe-S cluster assembly ATPase SufC", "db_xrefs": [ "GO:0005524", "GO:0016226", "SO:0001217", "UniParc:UPI000DBFF8DA", "UniRef:UniRef100_A0A328EG33", "UniRef:UniRef50_A0A288DFG5", "UniRef:UniRef90_A0A660MQI3" ], "nt": "ATGCTTCAAATCAAAAATCTTTCCGTGAAGGTCGGAGAAAAACAAATTCTAAATCAGATTTCTTTTGACTTTGAAACTGGGAAAAACTACTGTATCCTTGGGAAAAATGGCTCTGGGAAGTCCTCACTTGCGATGACCCTTGCTGGAAATCCCAAATATGAAATCAGCGAAGGCGAACTCCTTCTTGATGGACAGAATCTCACAGAAATGACTCCTGACGAGAGGAATAAAGCTGGAATTTTCCTGACTTTTCAAAATATCCCTGAGATCCCTGGGGTGAAATTGTTTGAGTTTCTCAAGACGATTTATGATGCGAGAGCTGAGAAACCAAGCACTTTTTTGGAGTTCAAAAAGATTATTGAGCCGCTGATGGCGGAGCTAGAGATTGAAAAAGCTTTTCTTTGGAGGGACTTGAATGTAGGGTTTAGTGGTGGAGAAAAAAGGAAAATTGAAGTCCTTCAGATTAAGCTTCTCAAGCCGAAATATATCATTCTTGATGAGATAGATAGCGGACTTGATGTGAATGCCGTGAGGCAGGTTGCTGAGCTTTTGAAAGCGGTAAATACGGAGGAAAATTCCTTTATTGTGATTACGCATCTTTTTGAAATGCTCCAAGAGCTTCCACTAGAAAAAATTCTGATCCTTGAGCAAGGAAAGATTAAAGCGATAGGGGGAAGAGAACTTATGGAACAGGTAAAGAAGGAAGGGTTTTAA", "aa": "MLQIKNLSVKVGEKQILNQISFDFETGKNYCILGKNGSGKSSLAMTLAGNPKYEISEGELLLDGQNLTEMTPDERNKAGIFLTFQNIPEIPGVKLFEFLKTIYDARAEKPSTFLEFKKIIEPLMAELEIEKAFLWRDLNVGFSGGEKRKIEVLQIKLLKPKYIILDEIDSGLDVNAVRQVAELLKAVNTEENSFIVITHLFEMLQELPLEKILILEQGKIKAIGGRELMEQVKKEGF", "aa_hexdigest": "4bffafc2072a53c570e7a2088b65b6d4", "start_type": "ATG", "rbs_motif": "TTCTT", "ups": { "uniparc_id": "UPI000DBFF8DA", "uniref100_id": "UniRef100_A0A328EG33", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBFF8DA", "UniRef:UniRef100_A0A328EG33" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EG33", "uniref90_id": "UniRef90_A0A660MQI3" }, "psc": { "uniref90_id": "UniRef90_A0A660MQI3", "gene": "sufC", "product": "Fe-S cluster assembly ATPase SufC", "uniref50_id": "UniRef50_A0A288DFG5", "go_ids": [ "GO:0005524", "GO:0016226" ] }, "pscc": { "uniref50_id": "UniRef50_A0A288DFG5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A288DFG5" ], "product": "ABC transporter domain-containing protein" }, "genes": [ "sufC" ], "id": "NKOCPLAKPK_524", "locus": "GCA003260355_00487" }, { "type": "cds", "sequence": "contig_1", "start": 257959, "stop": 258081, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATATAAACCTTCCACCAGAACTTTTTCAAGCTTATCTTTCTAAACTCCAACAAGACAATACTGATGAACAATTTGAGAAGTTTCAAGCTAATTCTCCAAGGATTATTAAAATCCATTGA", "aa": "MNINLPPELFQAYLSKLQQDNTDEQFEKFQANSPRIIKIH", "aa_hexdigest": "386756eb074059144e675c5706cff180", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4760.3417, "isoelectric_point": 5.538584709167479 }, "id": "NKOCPLAKPK_525", "locus": "GCA003260355_00488" }, { "type": "cds", "sequence": "contig_1", "start": 258286, "stop": 258396, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGAGCTTGTGTAGATTTTTCTTTAGTCTTCCTCATGGAGCTTAAAAAGCTTCTTCCTCAAGATAATCTTGCAATCGGATGTGAACTCCTGCGTCGAAAATCCACTTGA", "aa": "MGACVDFSLVFLMELKKLLPQDNLAIGCELLRRKST", "aa_hexdigest": "59d051ac8d71f12d012d0955d04b5904", "start_type": "ATG", "rbs_motif": "TTCTT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4053.8761000000004, "isoelectric_point": 7.80081958770752 }, "id": "NKOCPLAKPK_526", "locus": "GCA003260355_00489" }, { "type": "cds", "sequence": "contig_1", "start": 258661, "stop": 259215, "strand": "+", "frame": 1, "gene": "nth", "product": "endonuclease III", "db_xrefs": [ "EC:4.2.99.18", "GO:0006281", "GO:0140078", "SO:0001217", "UniRef:UniRef50_A0A7W4E3D1", "UniRef:UniRef90_A0A328EHZ4" ], "nt": "ATGAAACAACTTAAAAAACCAGATTTTAAGACTATTTTCTCTTACTTAGAGCAGCTTTTTCCAAATGCCGATACTGAGCTCAACTACGAAACGCCTTTTCAGCTGCTCGTTGCCGTGATTCTTTCTGCTCAAACGACCGATAAACAGGTCAATAAAGTCACAGCAACACTCTTTCAAACGATTAAACAGCCGGAAGATGTGCTGAATTTGGGGCATCCAGCTTTTGAGCAAGCGATTAAATCAATCGGACTCTACAAAGGCAAAGCAAAAAACATCTATCAGACTTCTGAACTTTTAGTTGAAAAAAAAGGAAAAATCCCAAAAACTGAAGCTGAACTCATGAAACTCCCTGGAGTCGGAGAAAAAACTGCTAAAGTCGTGCTTCATGTCCTTTATAAAAAGCCTGTAATCGCTGTAGATACGCATGTCCATAGGGTTTGCAATAGGCTTGGTATCGTAGATACTACAACTCCACTTCAGACTTCAAAACTTCTTGAAACTCTTATACCAAAAAAGTATAAACAGATTGCTCATCATTGTATTATCCTTTTTTGA", "aa": "MKQLKKPDFKTIFSYLEQLFPNADTELNYETPFQLLVAVILSAQTTDKQVNKVTATLFQTIKQPEDVLNLGHPAFEQAIKSIGLYKGKAKNIYQTSELLVEKKGKIPKTEAELMKLPGVGEKTAKVVLHVLYKKPVIAVDTHVHRVCNRLGIVDTTTPLQTSKLLETLIPKKYKQIAHHCIILF", "aa_hexdigest": "85ba7a1225505954d040701d45dd3b65", "start_type": "ATG", "rbs_motif": "TTTA", "psc": { "uniref90_id": "UniRef90_A0A328EHZ4", "query_cov": 1.0, "subject_cov": 0.8598130841121495, "identity": 0.995, "score": 357.0, "evalue": 2.52e-124, "valid": true, "gene": "nth", "product": "endonuclease III", "ec_ids": [ "4.2.99.18" ], "uniref50_id": "UniRef50_A0A7W4E3D1", "go_ids": [ "GO:0006281", "GO:0140078" ] }, "pscc": { "uniref50_id": "UniRef50_A0A7W4E3D1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7W4E3D1" ], "product": "Endonuclease III" }, "genes": [ "nth" ], "id": "NKOCPLAKPK_527", "locus": "GCA003260355_00490" }, { "type": "cds", "sequence": "contig_1", "start": 259433, "stop": 259903, "strand": "+", "frame": 2, "gene": null, "product": "Phosphatidic acid phosphatase type 2/haloperoxidase domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EGB9", "UniRef:UniRef90_A0A328EGB9" ], "nt": "GTGTATCCTGTTTTTTTGACCGTGCTTTATCTCTATGGAATGGTTAAAAAAAATCGAGAATCTAAGAAAGCTGCGCTCTTTATTTTCTTTGCCTGTTTTTTTTCGGTGGTGGTCAATATCCTTTCTCAACAGTTTTTTGATAAACAGAGGCCAATCCATGAACTCGGAATTGTAGATGTGGATCAAGAAACCTTGCTTCATCATCTTTTACCATTGACTTCGTTTCCGTCTGATCATGCGGTAGTTACCTTTTCGATTGCAATGGCGACATGGCTTATTTCTCATAAATATCGCAAAATGCATCTCAAAATTTGGTCATTCTTTTTGTTTGTGTTTGCGATTCTGACGGGGATTTGCAGAATTGGGACGAAAGTGCATTGGACTACTGATATTCTTGCAGGAGCAATTATGGGGCTTTTGGTACCAGTGGTTTTGGTGATTCCTTTCTTTTATAAGAGAATTGAAAGCTGA", "aa": "MYPVFLTVLYLYGMVKKNRESKKAALFIFFACFFSVVVNILSQQFFDKQRPIHELGIVDVDQETLLHHLLPLTSFPSDHAVVTFSIAMATWLISHKYRKMHLKIWSFFLFVFAILTGICRIGTKVHWTTDILAGAIMGLLVPVVLVIPFFYKRIES", "aa_hexdigest": "b7974e442e925b2aae71080432a81842", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 259307, "stop": 259963, "observations": { "mutation": [ 259340, 259373, 259901 ], "stop": [ 259340, 259373, 259901 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EGB9", "query_cov": 1.0, "subject_cov": 0.7155963302752294, "identity": 0.981, "score": 308.0, "evalue": 4.22e-105, "gene_start": 1, "gene_end": 156, "reference_start": 43, "reference_end": 198, "reference_sequence": "MPKIFMVEPEWGFDFLLAIAELGEKYPWVGNFYPIGADIFVFVYPVFLTALYLYGMVKKNRESKKVALFIFFACFFSVVVNILSQQFFDKQRPIHELGIVDVDQETLLHHLLPLTSFPSDHAVVTFSIAMATWLISHKYRKMHLKIWSFFLFVFAILTGICRIGTKVHWTTDILAGAIMGLLVPVVLVIPFFYKRIESGILIPIIRFEEWILGKIKKK" }, "paralog": false, "identity": 0.9678899082568807, "score": 429.0, "evalue": 2.66e-155, "description": "Internal stop codon at 259340, 259373, 259901. Nonsense mutation around 259340, 259373, 259901.", "psc": { "uniref90_id": "UniRef90_A0A328EGB9", "product": "Phosphatidic acid phosphatase type 2/haloperoxidase domain-containing protein", "uniref50_id": "UniRef50_A0A328EGB9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EGB9", "UniRef:UniRef50_A0A328EGB9" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EGB9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EGB9" ], "product": "Phosphatidic acid phosphatase type 2/haloperoxidase domain-containing protein" } }, "id": "NKOCPLAKPK_528", "locus": "GCA003260355_00491" }, { "type": "tRNA", "sequence": "contig_1", "start": 260037, "stop": 260108, "strand": "+", "gene": "trnT", "product": "tRNA-Thr(ggt)", "amino_acid": "Thr", "anti_codon": "ggt", "score": 58.0, "nt": "GCCGCGATAGTTCATTCGGTAGAACGCATCCATGGTAAGGATGAAAGACGAGTTCAAATCTCGTTCGTGGCT", "db_xrefs": [ "SO:0000270" ], "anti_codon_pos": [ 260054, 260056 ], "id": "NKOCPLAKPK_12", "locus": "GCA003260355_00492" }, { "type": "cds", "sequence": "contig_1", "start": 260209, "stop": 260586, "strand": "-", "frame": 1, "gene": null, "product": "HIT domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EJ99", "UniRef:UniRef90_A0A328EJ99" ], "nt": "ATGCAAATCAAATACCACGATATCGTTGCCCACAACCTAAAATATAATCTCTGTCCTTTTTGCGACATGCCACCAAAATTTATTTTGGAAAGCTCTCAATATTTTTTTGTTACCCTAGCTCGTGCCCCTTATATCAAAGACCATCTACTTATTATTCCACATAGGCACGTCGTGCTTTTCAATGATCTCTCCACCAAGGAAGTAAAAGACCTTATGTCCCTCGTCAGCAAACGAGACGACTTTCTCCACCAGACGCATTCCGATATCAATTTACTCCTGAGAGACGGATTTGTAGGAGGGAAAGTAGGGAAAAGTGTCAACCATATGCATTTTCACCTCATTCCTGACCTTGCTATTGGTCACGAAGAAAAAAGCTGA", "aa": "MQIKYHDIVAHNLKYNLCPFCDMPPKFILESSQYFFVTLARAPYIKDHLLIIPHRHVVLFNDLSTKEVKDLMSLVSKRDDFLHQTHSDINLLLRDGFVGGKVGKSVNHMHFHLIPDLAIGHEEKS", "aa_hexdigest": "e50ca674bc8ba027adc170923f765790", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A328EJ99", "query_cov": 1.0, "subject_cov": 0.8169934640522876, "identity": 1.0, "score": 262.0, "evalue": 1.23e-88, "valid": true, "product": "HIT domain-containing protein", "uniref50_id": "UniRef50_A0A328EJ99" }, "pscc": { "uniref50_id": "UniRef50_A0A328EJ99", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EJ99" ], "product": "HIT domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_529", "locus": "GCA003260355_00493" }, { "type": "cds", "sequence": "contig_1", "start": 260757, "stop": 261254, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03129.25", "PFAM:PF13393.11" ], "nt": "TTGAAACAGATTCAGGAAGCAATGCATCTTGAGTTTGAATGGAAAATTGATTTGCAAATTATCAGAGGACTAGACTATTATACGGGAACAATTTTTGAAGCCGTGCTCAAAAATGACGAAAAGCTCGGTTCTATCTGTGGAGGAGGAAGATATGCGAATTTGACTGGATATATTGATCCGAAAAGAAGCGATTATTGCGGAGTTGGAGGAAGTATCGGGATTAGTAGACTGCTCGGAAAAATTTTTGAGCTCAAAGAGGAAAAAAAGCAGACTGTTGCTGACTATCTTTTCCTCCATTTCGAAGAAAATTTTGAATCAATCCTAAAACTCGCAGGCACCTTCGCCAAAAGAAGTCAGACTTTTGAAATCTTTCCGACAGCAGAAAAACTCGGAAAACAATTTGCCTATGCCGACAAAAAAGGAATTCCTTACGTGATAATTTACGGACAAGGAGAAAAAGAAGCTGGCATCTACAAAATCAAAGATATGAAAACCTGA", "aa": "MKQIQEAMHLEFEWKIDLQIIRGLDYYTGTIFEAVLKNDEKLGSICGGGRYANLTGYIDPKRSDYCGVGGSIGISRLLGKIFELKEEKKQTVADYLFLHFEENFESILKLAGTFAKRSQTFEIFPTAEKLGKQFAYADKKGIPYVIIYGQGEKEAGIYKIKDMKT", "aa_hexdigest": "67c1b29b6acb4dd096e2c36f86b974b1", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03129.25", "name": "HGTP_anticodon", "length": 62, "aa_cov": 0.37575757575757573, "hmm_cov": 1.0, "evalue": 6.990017868534683e-10, "score": 35.44593811035156, "start": 104, "stop": 165 }, { "id": "PF13393.11", "name": "tRNA-synt_His", "length": 73, "aa_cov": 0.44242424242424244, "hmm_cov": 0.9178082191780822, "evalue": 9.616058487124178e-10, "score": 34.5903205871582, "start": 3, "stop": 75 } ], "seq_stats": { "molecular_weight": 18759.42379999999, "isoelectric_point": 7.67266139984131 }, "id": "NKOCPLAKPK_530", "locus": "GCA003260355_00494" }, { "type": "cds", "sequence": "contig_1", "start": 261348, "stop": 261833, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAGAATACGGATTACATTTTGATCTTACCGTGCCATTTGCGAGATATACGCTGGATTGGGAGCATCAGCTGACCTTTCCTTTCAAAAGGTACCAGATTCAGCCGGTTTGGAGAGGAGAAAGAGCTCAAAAAGGAAGATATAGAGAGTTTTTTCAATGCGATATTGATGTGATTTGGAAAGGAGAAAAGGACTATTTCTACTATGATGCAGAGATCATCGCAACACTCGGTTCTACGCTAAATAAAATCTTGAAAAGTTGCGATCTTCAAGATACTGCTGTTTTTCATATCAATAATAAGAAGCTCGTCAAAGCATTTCTCGCAGAAGTCAGTCAAGATGAAGAGCAAATGCAACAGATTTCAACCCTTATTGATAAGTACAAGAAGATTGGAACAGAAAACTTTATCGCAACACTCAGAGAAGACCTCAATATCTCAGCAGAAAATAGCGAAAAGATCCTGAATTTTATCTCTTTTCAGTGA", "aa": "MKEYGLHFDLTVPFARYTLDWEHQLTFPFKRYQIQPVWRGERAQKGRYREFFQCDIDVIWKGEKDYFYYDAEIIATLGSTLNKILKSCDLQDTAVFHINNKKLVKAFLAEVSQDEEQMQQISTLIDKYKKIGTENFIATLREDLNISAENSEKILNFISFQ", "aa_hexdigest": "21b16f40f3682d631247ee9a82cb1563", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19124.536300000007, "isoelectric_point": 5.662493705749512 }, "id": "NKOCPLAKPK_531", "locus": "GCA003260355_00495" }, { "type": "cds", "sequence": "contig_1", "start": 261951, "stop": 262055, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAGAACTTCGTCCTAGTGGATTCCCTGAATATAGTGCTAGTCAGCAGTTAATTTTTGATCAGCTCAAAGAAACCATCGAGCAGAGCTATCAGCAGTTTTGA", "aa": "MAELRPSGFPEYSASQQLIFDQLKETIEQSYQQF", "aa_hexdigest": "5193ed5fa0a1cd00b5aac6c25abe64cd", "start_type": "ATG", "rbs_motif": "TTATT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4010.3943, "isoelectric_point": 4.251295280456543 }, "id": "NKOCPLAKPK_532", "locus": "GCA003260355_00496" }, { "type": "cds", "sequence": "contig_1", "start": 262444, "stop": 262773, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATTTTGTCAGGATTGTCGTGGTATTCGGGGGTTTCTGTGGCACTCTTTTGTGGAAATCTCTTCCTCCCGTTTGTAAAGTGGATCCATGTGGTTTTTTCAGGTTTTTTTGTTGTTGCATTGGTTATTGAGCTGAGTGGAATCCTAAAATTTTTTCTAAAGGTCGTGATGGAAAAGGGATTTGCTCATCAGGACACGAAAACTAAGAAAAATTTGACTGAAATCCTTAATACGCTTCTTCAGATTCTGATTTGGATATTGGCGGCATTGATTTTCTTTGATACGGTGGGGGTTGCGATTACGCCGCTCCTAGCATCGCTTGGAGTATGA", "aa": "MILSGLSWYSGVSVALFCGNLFLPFVKWIHVVFSGFFVVALVIELSGILKFFLKVVMEKGFAHQDTKTKKNLTEILNTLLQILIWILAALIFFDTVGVAITPLLASLGV", "aa_hexdigest": "fa5fb30beb4c3ef8f07c3bab5a83d76c", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12046.45659999998, "isoelectric_point": 8.891108894348147 }, "id": "NKOCPLAKPK_533", "locus": "GCA003260355_00497" }, { "type": "cds", "sequence": "contig_1", "start": 262837, "stop": 263313, "strand": "+", "frame": 1, "gene": "mscS", "product": "Mechanosensitive ion channel protein MscS", "db_xrefs": [ "KEGG:K16052", "SO:0001217", "UniRef:UniRef50_A0A2W4IGW0", "UniRef:UniRef90_A0A328EG83" ], "nt": "ATGGGATCGAGTCCCTTTAGGATTGGTGATATTATTACGATCAAAGATACAACTGGGACGGTCAAAAAGATTGGACTGAGAAGCACTACGCTCCTTACGGTGGAGGATAAATCGATCATTATTCCCAATAGACTTTTGGTCGCAGAAATTATCGAAAACAGTGGAACAATCAAAGTCAGAAGAAAGAGATTTTCACTGACGATCACGTATGAGACGCCGCTTGAAAAGGTGCGTCAGATTCCTAAGCTTTTGGAGCAAATTATTGGGAAGCATAAGGATGTTGGATTTGAATGGGCGGTGCTGAGGGATTTGCAAGCATCGTGCTTGGAGATTTATGTTAGTTATGTGATTAATAATGATGATTGGCTAAATGCTACCCAGCTTCATAGTAAAATCCTGGAAGAAATCCTAGAAAAATTTGCGGAGGAGAAGATTGATTTTGCCTATCCAACTCAAACGCTCCGACTCAAGAAATAA", "aa": "MGSSPFRIGDIITIKDTTGTVKKIGLRSTTLLTVEDKSIIIPNRLLVAEIIENSGTIKVRRKRFSLTITYETPLEKVRQIPKLLEQIIGKHKDVGFEWAVLRDLQASCLEIYVSYVINNDDWLNATQLHSKILEEILEKFAEEKIDFAYPTQTLRLKK", "aa_hexdigest": "fb5b9024cfb33ee3750a3a9c96d67048", "start_type": "ATG", "rbs_motif": null, "genes": [ "mscS" ], "truncated": "5-prime", "pseudogene": { "start": 262537, "stop": 263313, "observations": { "mutation": [ 262771 ], "stop": [ 262771 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EG83", "query_cov": 1.0, "subject_cov": 0.45664739884393063, "identity": 1.0, "score": 304.0, "evalue": 1.11e-101, "gene_start": 1, "gene_end": 158, "reference_start": 189, "reference_end": 346, "reference_sequence": "MEFSFFREDATVPSMQDFLLAGGVVVGFFVLFCLIKFGARFLLARLGKGASLKKDFFVILSGLSWYSGISVALFCGNLFLPFVKWIHVVFSGFFVVALVIELSGILKFFLKVVMEKGFAHQDTKTKKNLTEILNTLLQILIWILAALIFFDTVGVAITPLLASLGVGGIAVAFASQKLIEDFFSSFSIMGSSPFRIGDIITIKDTTGTVKKIGLRSTTLLTVEDKSIIIPNRLLVAEIIENSGTIKVRRKRFSLTITYETPLEKVRQIPKLLEQIIGKHKDVGFEWAVLRDLQASCLEIYVSYVINNDDWLNATQLHSKILEEILEKFAEEKIDFAYPTQTLRLKK" }, "paralog": false, "identity": 0.9961240310077519, "score": 492.0, "evalue": 4.62e-178, "description": "Internal stop codon at 262771. Nonsense mutation around 262771.", "psc": { "uniref90_id": "UniRef90_A0A328EG83", "product": "Mechanosensitive ion channel protein MscS", "uniref50_id": "UniRef50_A0A2W4IGW0", "kegg_orthology_id": "K16052", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EG83", "UniRef:UniRef50_A0A2W4IGW0", "KEGG:K16052" ] }, "pscc": { "uniref50_id": "UniRef50_A0A2W4IGW0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4IGW0" ], "product": "Mechanosensitive ion channel family protein" } }, "id": "NKOCPLAKPK_534", "locus": "GCA003260355_00498" }, { "type": "cds", "sequence": "contig_1", "start": 263335, "stop": 263904, "strand": "+", "frame": 1, "gene": "rsmG", "product": "16S rRNA (guanine(527)-N(7))-methyltransferase RsmG", "db_xrefs": [ "EC:2.1.1.170", "GO:0008168", "GO:0031167", "GO:0046118", "KEGG:K03501", "SO:0001217", "UniParc:UPI000DBF83A7", "UniRef:UniRef100_A0A328EK42", "UniRef:UniRef50_A0A288DFT4", "UniRef:UniRef90_A0A328EK42" ], "nt": "ATGCGAGATAAATTGATCACTATTTTTTTGGAACAGAATCAAAAACTGAATCTATCTGCGATTCGTGATAGGGAAGGTGTCAGGGTGAAGCATCTTCAGGATTCTCTCAAACTTCTCGAAACCGGTCTCTTTACTCCGGGGAAGTTTGTGATTGATGTCGGGACGGGGGGCGGCTTCCCGTTGATGCCTTTAGCGATGAGCTGTCCTGAGATGTGTTTCCTCGGAATTGACTCGGTAAGGAAGAAGACGCTTGCTGTGCAAGCAATGCTTGGTGAGCTTGGCGTGCAAAATGCTGAGGTATTGCGAACTAGGATTGAGGACTACCAGGGAGAAAAGGCTGACCTTTTGACTGCGAGAGCGGTTGCCTATAGCGATAAACTTTTGCAGTGGAGCGTTCCGTTGGTGAAGAAAGGCGGAGCTTTGGTATTGATGAAACAAGTTATTCCTGAAGAAAAAAGGCTTTTGCAAGGGCTCTGTCCTCGTTTTCAGCTTAAGATTGAGCAGGAGTTGAAGTATCAGCTGTTTGAGGGGGATATTGAGAGAGTGATCTATGTGCTGAGGAAAAAGTAA", "aa": "MRDKLITIFLEQNQKLNLSAIRDREGVRVKHLQDSLKLLETGLFTPGKFVIDVGTGGGFPLMPLAMSCPEMCFLGIDSVRKKTLAVQAMLGELGVQNAEVLRTRIEDYQGEKADLLTARAVAYSDKLLQWSVPLVKKGGALVLMKQVIPEEKRLLQGLCPRFQLKIEQELKYQLFEGDIERVIYVLRKK", "aa_hexdigest": "1ef670b68b51d9d4b8c81fcf1922359c", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000DBF83A7", "uniref100_id": "UniRef100_A0A328EK42", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBF83A7", "UniRef:UniRef100_A0A328EK42" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EK42", "uniref90_id": "UniRef90_A0A328EK42" }, "psc": { "uniref90_id": "UniRef90_A0A328EK42", "gene": "rsmG", "product": "16S rRNA (guanine(527)-N(7))-methyltransferase RsmG", "ec_ids": [ "2.1.1.170" ], "uniref50_id": "UniRef50_A0A288DFT4", "kegg_orthology_id": "K03501", "go_ids": [ "GO:0008168", "GO:0031167", "GO:0046118" ] }, "pscc": { "uniref50_id": "UniRef50_A0A288DFT4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A288DFT4" ], "product": "Ribosomal RNA small subunit methyltransferase G" }, "genes": [ "rsmG" ], "id": "NKOCPLAKPK_535", "locus": "GCA003260355_00499" }, { "type": "cds", "sequence": "contig_1", "start": 264094, "stop": 264885, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DC048A1", "UniRef:UniRef100_A0A328EG97", "UniRef:UniRef50_A0A328EG97", "UniRef:UniRef90_A0A328EG97" ], "nt": "ATGGGACTCTATTCACCAGCAGAACTTGTTATCAACACGCTCTTGGAAGTCTTCGCTCGAGCAGTCGGATTCACAATCTTAGTCTACGCCATCACCGCATTCCTCAAGAAAAAATCCAGAATAATCAGAGGATTGAGAAAATTCTTCTTGAGTATCACGATGATTACAGGAGCTTGCTTCGCCATCATCGGGCTCATGCACGCAGTCGGAATCAAAGAAGATATTAAAAGAGGAGAAACCGTAATTTTTGGAGCTAGAGACACATTTTATATGAAGTTGATGGAGCTTAATAAACAAGAAAGAGAAAAAGAAAACAGTCGGAAGCCTGAACCTTTCGGAGGCTATCGTCAAGAGGAGGTAAAAACAGTGCCAATCTCCTCTGGTATGAGGATAGCAGCCAATCCAGAGAAAGCAGAGATCCTAAATTTAGAAATCGAGCCACAAAGAGACAGAGAAATCAGACTCACTTTCAAAAATGCAAGATTCCGATTAGAGAATATGGAGCTCATGAATCCTTATCCAGAAAAACAATCCGCATACGATAAACTCGACTATCATTACCTTGCTCAAGACTATTATTCTAATACATTGAGGAAAAAATACCAAGGAGATTTTTGTATCCAACTCTACGACAGTGCTGTGATCACCAATGAAATGAAAGAAAAGAGTGGAAATTGTGATATTATCACGCTCGCAGGTGGTGCAAAAACACATCTAAGAATGAGTCTCGCTACAGAAGATAAACTCCAGCCTTCTTATATGGAAGTCGAATATCTTCCCCTAGCAAACTAA", "aa": "MGLYSPAELVINTLLEVFARAVGFTILVYAITAFLKKKSRIIRGLRKFFLSITMITGACFAIIGLMHAVGIKEDIKRGETVIFGARDTFYMKLMELNKQEREKENSRKPEPFGGYRQEEVKTVPISSGMRIAANPEKAEILNLEIEPQRDREIRLTFKNARFRLENMELMNPYPEKQSAYDKLDYHYLAQDYYSNTLRKKYQGDFCIQLYDSAVITNEMKEKSGNCDIITLAGGAKTHLRMSLATEDKLQPSYMEVEYLPLAN", "aa_hexdigest": "9643557898f680dd478f3d0d07a657fa", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000DC048A1", "uniref100_id": "UniRef100_A0A328EG97", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DC048A1", "UniRef:UniRef100_A0A328EG97" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EG97", "uniref90_id": "UniRef90_A0A328EG97" }, "psc": { "uniref90_id": "UniRef90_A0A328EG97", "uniref50_id": "UniRef50_A0A328EG97" }, "pscc": { "uniref50_id": "UniRef50_A0A328EG97", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EG97" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 30241.757200000015, "isoelectric_point": 8.762301063537599 }, "id": "NKOCPLAKPK_536", "locus": "GCA003260355_00500" }, { "type": "cds", "sequence": "contig_1", "start": 266077, "stop": 266586, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF19310.4" ], "nt": "TTGGAACAGGAAAAAGCTGATTTTCAGACCTTAATCCAAAAAAACAATGAAAGTCTGACTTTTGAGGATTTTGTGCCAGAATCTCAGCTTGCTTACCTCTGGAAAATCATCAATCATCTTGATGCGGTAGAAGCAAGCGAAGAAATCAGAGCGATTATTGCAGATTTTAGACCAAAGTACGAGGATTTTGTGAATGAAGTGGCTTATTCTCAGCTTTATTATCAGAAGCTGCTCCGAGCAAGCGAACATTTTGAAAAGAATCCTCATCAGCAGAGGTGGTTTGATTTAACTTTGAAAGCTTTTGAACAAAGGGGAATCGCATTGCCAGCAGAGGAGCAAGCACAGCTCAAAAATCTCAATAAACAGCTTTCGGAGCTGGGAGAGAAGTTTCAGCATAATGTCGTAGATGAACAGTCAGCTTTTCTGCTTTATTTTGATAATGAGGAGGATTTTCAGGAGATGCCGGAGGAACTCTTGGCGAAAATGAAAGCACTTGCTGGGGAGAAATGA", "aa": "MEQEKADFQTLIQKNNESLTFEDFVPESQLAYLWKIINHLDAVEASEEIRAIIADFRPKYEDFVNEVAYSQLYYQKLLRASEHFEKNPHQQRWFDLTLKAFEQRGIALPAEEQAQLKNLNKQLSELGEKFQHNVVDEQSAFLLYFDNEEDFQEMPEELLAKMKALAGEK", "aa_hexdigest": "2fabf032aedda0b3d242ea4ae65cb5fd", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF19310.4", "name": "TOP_N", "length": 88, "aa_cov": 0.5029585798816568, "hmm_cov": 1.0, "evalue": 7.325274277088532e-13, "score": 44.865535736083984, "start": 28, "stop": 112 } ], "seq_stats": { "molecular_weight": 19965.12779999999, "isoelectric_point": 4.6489408493042 }, "id": "NKOCPLAKPK_537", "locus": "GCA003260355_00501" }, { "type": "cds", "sequence": "contig_1", "start": 266614, "stop": 267096, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTTGTTGCGGTAAGTAAATATTGCAGTAATCCAGAAATTAGAAAGCAGGTGGCTTTTCAGAGAGAGCAATGGGCGAGTGAGGGAAGATTTGACAATAGACCTTTGGCTTTGGAGCTTCTAAAACTCAATCAAGAGAAAGCAAAGATTCTTGGCTATGCGAATGCAGGAGAAATGTTCCTTTGGGCTACAATGGCTCAAGATCCGAATCAGGTTTTTGATTTTATCAAGCCGATCTCTGCGAAGGCGATGCAAAAAGCGAAATCTGAAGTGCAGACTTTGCAAGAGTATTTTCAGCTTCCAGATTTGCCAAGCTACGATCTCGCCTACTATACGAGGAAGTATAAGGAAGAGCAATATGCCCTCAATGATGAGGAACTCAAGCAGTATTTTGAGTTTGAGTCGGTTTTGAAGTGGCTCCATAGCTTTGTATTCAAGTTTTTAGGTGTGGAACTTCGCGCTAGAGCAGAATCAGCGTTTTGA", "aa": "MLVAVSKYCSNPEIRKQVAFQREQWASEGRFDNRPLALELLKLNQEKAKILGYANAGEMFLWATMAQDPNQVFDFIKPISAKAMQKAKSEVQTLQEYFQLPDLPSYDLAYYTRKYKEEQYALNDEELKQYFEFESVLKWLHSFVFKFLGVELRARAESAF", "aa_hexdigest": "2415f85c71df12aba140cb27fed3121b", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18831.325399999994, "isoelectric_point": 5.9507242202758786 }, "id": "NKOCPLAKPK_538", "locus": "GCA003260355_00502" }, { "type": "cds", "sequence": "contig_1", "start": 267289, "stop": 267489, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTCTATCTGAGAGATGTTGAGACGCTATTTCATGAGTTTGGACACGCTTTGCATGCGATGTTGGCGGGTTCTCCCTATGCGGAGCTTTCTGGATTTAATGTAGAATGGGATTTTGTGGAGCTTCCAAGTCAATTGATGGAAAATTGGGTGAGCGATCCTGAAGCCTTGAAAAGTTTAGCAAAGCACTATCAAACCTGA", "aa": "MLYLRDVETLFHEFGHALHAMLAGSPYAELSGFNVEWDFVELPSQLMENWVSDPEALKSLAKHYQT", "aa_hexdigest": "2120dc7a243da88fe345cd4d500588de", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7611.510399999998, "isoelectric_point": 4.686909294128417 }, "id": "NKOCPLAKPK_539", "locus": "GCA003260355_00503" }, { "type": "cds", "sequence": "contig_1", "start": 267544, "stop": 267810, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGAAACTGGGTAAGCAGGCAAAATGAGTTAGCTTTGCTGGATATGACGCTCTACACTTCCGATATCCCAAACTCGGTAGAAGAATTGGATCAAAAGACTCTTGGAATCGTGAATCAATATTCCATTTTTCCAAGAGGGGAGGAATACAAGATGTATTGTGCGTTTCTGCATATTTTCGGAGGTGGCTACGAAGCGAAGTATTATTCCTATATGCGAGCAGAGCTTTTGGAGGCTGATGTATTTCAAACCATCAAAAAAACCTGA", "aa": "MGNWVSRQNELALLDMTLYTSDIPNSVEELDQKTLGIVNQYSIFPRGEEYKMYCAFLHIFGGGYEAKYYSYMRAELLEADVFQTIKKT", "aa_hexdigest": "578928aab689480061a21b3063273296", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10248.543699999995, "isoelectric_point": 4.810988807678222 }, "id": "NKOCPLAKPK_540", "locus": "GCA003260355_00504" }, { "type": "cds", "sequence": "contig_1", "start": 268017, "stop": 268178, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAATTGCGTTTGTGAATTGCTGGACGATGCCAGGTGGTGTGCTCAATGTCCTTCAAGATTTGATGATTAAACAGCTGAGAAAGCATGGGAAGGAGGTGGAAGTCAGGCTTTTTACGCTTATTTCGGATCGTGAAAGTCTAGAACTTCCCTTGCCATGA", "aa": "MKIAFVNCWTMPGGVLNVLQDLMIKQLRKHGKEVEVRLFTLISDRESLELPLP", "aa_hexdigest": "90b891075fc3bea7b6c43d61d0600d0c", "start_type": "ATG", "rbs_motif": "TTATT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6108.269699999997, "isoelectric_point": 7.941038322448731 }, "id": "NKOCPLAKPK_541", "locus": "GCA003260355_00505" }, { "type": "cds", "sequence": "contig_1", "start": 268191, "stop": 268421, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTAACGATTCCGATTGTGCAAACCTTACCAAAATGGATCAATCAGATTTTTCTTTTTTTTACTAAGAGGAAAGTGAAGATTTTGTCTGGTGTTTTTGATTATCGTAATTTGATGGTGTTTTATCCTCGGCTTATGAAGCTCCTTTCTTTCAAGATCAAACAACGAAAGCCTGATGAGATTCAGATTTCTTCTTTTGCTATTGCGAAAAATATTACACCGATTCCGTGA", "aa": "MVTIPIVQTLPKWINQIFLFFTKRKVKILSGVFDYRNLMVFYPRLMKLLSFKIKQRKPDEIQISSFAIAKNITPIP", "aa_hexdigest": "4bd14422bc73998000666662f12a3485", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9013.897999999994, "isoelectric_point": 10.61267261505127 }, "id": "NKOCPLAKPK_542", "locus": "GCA003260355_00506" }, { "type": "cds", "sequence": "contig_1", "start": 268578, "stop": 268682, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGTTTGCAATAGTCATTATACTCAGGAACTTGCAGAGGAGCTTTATGGCCTCAAAAGTACGGTTTCCTATCCAAAGATCGCAGATCAGTATTGGTTTGCTTGA", "aa": "MVCNSHYTQELAEELYGLKSTVSYPKIADQYWFA", "aa_hexdigest": "cfc318c27ad96035f54ce0974d3f6bdd", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3986.4373000000005, "isoelectric_point": 4.830996131896971 }, "id": "NKOCPLAKPK_543", "locus": "GCA003260355_00507" }, { "type": "cds", "sequence": "contig_1", "start": 268698, "stop": 268862, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCTTATTTTGTGTATGTAGGGAGGCTGGTCAATTTTGTGAGGGAAACGGGCTTGATTATTCAGCTTTTTAATCGTTTGAAATTGCCTTTGGTGGTGATTGGTTCTGGACCTGATGAGGAGGAACTCAAAGCTTTGGCGGGTGAGACGGTTATTTTCACTTGA", "aa": "MPYFVYVGRLVNFVRETGLIIQLFNRLKLPLVVIGSGPDEEELKALAGETVIFT", "aa_hexdigest": "26882c981a48b82dedfe526c58ff01de", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6067.101499999999, "isoelectric_point": 5.1057558059692365 }, "id": "NKOCPLAKPK_544", "locus": "GCA003260355_00508" }, { "type": "cds", "sequence": "contig_1", "start": 268962, "stop": 269144, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGAGTTCCAGTATTTGGTTTCGCTGAAGGTGCGAGTGCAGAATTGGTGGATGAGGATTGTGGAGTTTTGGTGAAGAGTAAGGATTTAGAAGATTTGGTGTTAGATTTTGAACTCTTTCGTAGTAAAAGCCGAGATAGGAAGCTTATTTCTGAGAGGATTAGAGAAAAGCTGACTTCCTAG", "aa": "MGVPVFGFAEGASAELVDEDCGVLVKSKDLEDLVLDFELFRSKSRDRKLISERIREKLTS", "aa_hexdigest": "072d4c05db8bf0ac8af78e024368ab27", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6745.645599999996, "isoelectric_point": 4.821617698669433 }, "id": "NKOCPLAKPK_545", "locus": "GCA003260355_00509" }, { "type": "cds", "sequence": "contig_1", "start": 269457, "stop": 269693, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03382.20" ], "nt": "ATGGTGACTGCACTTCAGCTTACGGGCGAGGAAATTACTAAGGTAGAATTTAAGAATAATGTCTGGTTCCCGAACGTTGCAAATGGCTTATTTCAAAATTTTAAGAGTCAGATTATTTTCCCATTAACTCAATATACGAGTAATGTTTGGTCGATGCTGAATATGTTTAATGGGGCAGTAAAATTTAATTCCGACATTTCAAACTGGGATGTGAGTAATGTTACCGATATGTATTGA", "aa": "MVTALQLTGEEITKVEFKNNVWFPNVANGLFQNFKSQIIFPLTQYTSNVWSMLNMFNGAVKFNSDISNWDVSNVTDMY", "aa_hexdigest": "94b8f21688c007329ebbd53f9abe403b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03382.20", "name": "DUF285", "length": 32, "aa_cov": 0.41025641025641024, "hmm_cov": 1.0, "evalue": 8.797123938622905e-11, "score": 38.083377838134766, "start": 46, "stop": 77 } ], "seq_stats": { "molecular_weight": 8990.1024, "isoelectric_point": 4.593750190734863 }, "id": "NKOCPLAKPK_546", "locus": "GCA003260355_00510" }, { "type": "cds", "sequence": "contig_1", "start": 270126, "stop": 270230, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03382.20" ], "nt": "GTGGAGAATTTCGGTTTTATGTTTGAAAAAGCTAAGAAGTTCAATCAGCCAATAGGAAATTGGAATACGAGTAAAGCGACCTATATGGCGTATATGTTTTGGTGA", "aa": "MENFGFMFEKAKKFNQPIGNWNTSKATYMAYMFW", "aa_hexdigest": "752b4dae0ac572c74f0ce5516055865d", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03382.20", "name": "DUF285", "length": 31, "aa_cov": 0.9117647058823529, "hmm_cov": 1.0, "evalue": 7.402030862627063e-11, "score": 38.32538604736328, "start": 3, "stop": 33 } ], "seq_stats": { "molecular_weight": 4159.7855, "isoelectric_point": 9.303642082214353 }, "id": "NKOCPLAKPK_547", "locus": "GCA003260355_00511" }, { "type": "cds", "sequence": "contig_1", "start": 270267, "stop": 270443, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03382.20" ], "nt": "GTGAGTAAAGTTACTAATATGGATTCTATGTTTAATGCAGCGACAAGCTTTAATCAAAATCTTTCTACTCGAGATGTAAGTAATGTTGTAGACATGGGAGGAATGTTTGCCAATGCCTCTTCCTTCAATGGAGATATTTCCAATTGGAATACGAGTAAGGCGCAGCGTATGTGGTGA", "aa": "MSKVTNMDSMFNAATSFNQNLSTRDVSNVVDMGGMFANASSFNGDISNWNTSKAQRMW", "aa_hexdigest": "3e74be10f7de15ad6ac88a7475f722dc", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03382.20", "name": "DUF285", "length": 56, "aa_cov": 0.9655172413793104, "hmm_cov": 1.0, "evalue": 3.4143693737758685e-20, "score": 68.45478820800781, "start": 2, "stop": 57 } ], "seq_stats": { "molecular_weight": 6416.047600000002, "isoelectric_point": 5.853870582580566 }, "id": "NKOCPLAKPK_548", "locus": "GCA003260355_00512" }, { "type": "cds", "sequence": "contig_1", "start": 270454, "stop": 270708, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTCGTAGCTTTACTGGAGATGTTTCTGCTTGGGATTTTACTAAGGTTTATAATCCCTGACTGCCAGCTCAGGCTGGTTTCTCTATTAGGGCTCCTGGTAAGAATCGATATTGGTATCCGAGTGAAAATTATAAGAAGATTTTGCAGGCTATTAAAGTTGCTTATACTGCGGGGACACTTAATTTCTCTAAGTTTAGAATATTGTGAGTTGAATCTACCTATTGTGATTTCTATGGGCTTAGAGATGAACTGA", "aa": "MLVALLEMFLLGILLRFIIPDCQLRLVSLLGLLVRIDIGIRVKIIRRFCRLLKLLILRGHLISLSLEYCELNLPIVISMGLEMN", "aa_hexdigest": "cf2cea182a9b26336c7eedd1b452f965", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9671.059899999995, "isoelectric_point": 9.491631889343264 }, "id": "NKOCPLAKPK_549", "locus": "GCA003260355_00513" }, { "type": "cds", "sequence": "contig_1", "start": 270720, "stop": 271025, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAAATAGTCAGTGATAAATTTGACTGTGAGCCGAAAATCACGGCTACTGCACCTACCTTACAGAGTAGTGGAGAAATTACGGATACTAGGATTACTTTCAGGAGTGAGTTTCCTTTGACACAAACTGATCTAGATAATGGAGCACTTTCTGTAGATCCTGTAGGAACAACCGTTCAATACTCGAATTTTAACTGTAGTTTAGAACCCTTAGATGCTACTAAGGTAAACTGTAGCGTTAAGATTACTTCTACACAAGAACAAAGCGATAAAAATCTGAAACTTAAATTCTATAAGAATCTCTGA", "aa": "MQIVSDKFDCEPKITATAPTLQSSGEITDTRITFRSEFPLTQTDLDNGALSVDPVGTTVQYSNFNCSLEPLDATKVNCSVKITSTQEQSDKNLKLKFYKNL", "aa_hexdigest": "f3d451317c3c084d3e3915cae95dcf75", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11183.42279999999, "isoelectric_point": 4.760231590270995 }, "id": "NKOCPLAKPK_550", "locus": "GCA003260355_00514" }, { "type": "cds", "sequence": "contig_1", "start": 271704, "stop": 271886, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGACGGGAGGTGATCTTGCCTTTACGGTAATCGCTACGGATGATCAAGGAATCGCTGCTACAGGAGTTCAAATTACTTCTCCAGGTGCAGCAATGAATATCCAAAACCTTGCTTGTGTTCAGGTGAATCCTACGAAGGTGCAATGTGATTTTACGGTAAGTGATCCTGTGATTAGCTGA", "aa": "MLTGGDLAFTVIATDDQGIAATGVQITSPGAAMNIQNLACVQVNPTKVQCDFTVSDPVIS", "aa_hexdigest": "8240f7bf7ede0dee61f223718d9de58d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6109.888200000003, "isoelectric_point": 4.0500284194946286 }, "id": "NKOCPLAKPK_551", "locus": "GCA003260355_00515" }, { "type": "cds", "sequence": "contig_1", "start": 272130, "stop": 272264, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAATTGTAGTGGATATATGAGTTTAGATGGGGTGGCTCCTGGAGCTACAGAACCCTTTACCCTCAACTTTGAAATTGATGATGCAAATCAGAATGGGAAGTATCTTTGTGTAGTAGTAAAAGATAAAGTCTGA", "aa": "MNCSGYMSLDGVAPGATEPFTLNFEIDDANQNGKYLCVVVKDKV", "aa_hexdigest": "25f6f22530ad33c0e25e81a989f5da0b", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4755.338300000002, "isoelectric_point": 4.232879447937012 }, "id": "NKOCPLAKPK_552", "locus": "GCA003260355_00516" }, { "type": "cds", "sequence": "contig_1", "start": 272412, "stop": 272993, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00028.23", "PFAM:PF05345.18" ], "nt": "TTGAGCTACCAGATTATCGGTGGAAATACCGGCAACGCCTTTTCAATCGTTGCAGATAAACTGCAGGTAAATGGAGTACTTGATTATGAGACCTTGAGTGGCTATCTCTTAACAATTCAACTCAAAGATCAGCTCGGATTAACAGGAGAAGCGAAGGTTACGATTACTTTGAATGATGTGGATGAGATTCCGCCAGTGATTAGTCCTCTTACTGGAGTGAGCTATCAACGTTCTACGCCTATTGTGCCTATTCCATTGGTAGCAACTGATAATAGTGGATCGGTAACGCTTCAGGTAAACGGATTACCAGCAGGATTGACCTTTGATATGACGGGTTCTCAAATTATTGGAACGACTCCAGCTACTCCAGGAAGCTATCCTATCCAAGTGGTTGCGATTGATCAGGCAGGAAACCAGACTACCGTTCAGTTTACACTTACTGTGATAGCTCCTCCAGCTCCTAGTAGTAGTTCAAGTTCCTCTTACCGTTCTGGTGGAGCAGGAACAAGCTTACCAAAAGATAATTGTCCTAACGGTGATTTCTCATCAAGCTATTATGATGGTAGCTGTGGAAATGAGTGA", "aa": "MSYQIIGGNTGNAFSIVADKLQVNGVLDYETLSGYLLTIQLKDQLGLTGEAKVTITLNDVDEIPPVISPLTGVSYQRSTPIVPIPLVATDNSGSVTLQVNGLPAGLTFDMTGSQIIGTTPATPGSYPIQVVAIDQAGNQTTVQFTLTVIAPPAPSSSSSSSYRSGGAGTSLPKDNCPNGDFSSSYYDGSCGNE", "aa_hexdigest": "804245893d327eeeaef9c7a252e22e54", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00028.23", "name": "Cadherin", "length": 61, "aa_cov": 0.29015544041450775, "hmm_cov": 1.0, "evalue": 5.086508112405676e-10, "score": 36.139305114746094, "start": 2, "stop": 57 }, { "id": "PF05345.18", "name": "He_PIG", "length": 89, "aa_cov": 0.42487046632124353, "hmm_cov": 0.9887640449438202, "evalue": 1.2828712001617098e-13, "score": 47.707767486572266, "start": 67, "stop": 148 } ], "seq_stats": { "molecular_weight": 19881.910199999977, "isoelectric_point": 4.072593498229981 }, "id": "NKOCPLAKPK_553", "locus": "GCA003260355_00517" }, { "type": "cds", "sequence": "contig_1", "start": 273099, "stop": 273557, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAGAACTCTTGAGACAAGCTCATCAGATGCTCTATAGTTATCAGCTTACAAGATGGCAAGGCACCAAAGACTTTGCTCCAGAGAGAAGCTTAACCAGACAAGAAGCAGCAAGATTTATGACTGAATTTGCTACAAATGTGCTTTGTAGGAAACCAAGTAGAAACTATGCACATCAGTTCATAGACTTAGGAGATGCTGATCCAACGCTTCTACCTTATATCTATAAATCGTATGATTATCTTATCTTTAATGGGGATGGGAATCCAAACGGAGATAAAGCACAAACGACGTTTAGACCTTATGATCTGATAACGGTAGATGAACTCTCTGCACTTCTCACCAGACTCGTGAAGAATCAAACCATGGAAGAGCCAGCAGAAGATCGAGCAAGAAATTATAGGAATTATATTAGTTCTATCGCCTCAAACTCAGCTTTAAAGAATGATATTAGATGA", "aa": "MTELLRQAHQMLYSYQLTRWQGTKDFAPERSLTRQEAARFMTEFATNVLCRKPSRNYAHQFIDLGDADPTLLPYIYKSYDYLIFNGDGNPNGDKAQTTFRPYDLITVDELSALLTRLVKNQTMEEPAEDRARNYRNYISSIASNSALKNDIR", "aa_hexdigest": "20b5e1bdb9b627a2d1bfcbd6fa6b8696", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17671.622299999988, "isoelectric_point": 6.733056068420409 }, "id": "NKOCPLAKPK_554", "locus": "GCA003260355_00518" }, { "type": "cds", "sequence": "contig_1", "start": 273902, "stop": 274072, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATTCAATCTATACAAAAGATTATCAAAACAAGAAAATCGTTCCTCTCCTCCAGGAATACTCTGCAAAAAAATACGGAAGAGCAAGGAAATTCGTTGATGCAGAAATCTCTACCAGACTTGGTATGGGAGCAGATTTGGAAGACCTCCCAGATCTAGGAACTCCATGA", "aa": "MNSIYTKDYQNKKIVPLLQEYSAKKYGRARKFVDAEISTRLGMGADLEDLPDLGTP", "aa_hexdigest": "b38401e58dc9eac527fac59daa77969e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6350.193800000001, "isoelectric_point": 7.983394050598145 }, "id": "NKOCPLAKPK_555", "locus": "GCA003260355_00519" }, { "type": "cds", "sequence": "contig_1", "start": 274280, "stop": 274492, "strand": "-", "frame": 3, "gene": "traM", "product": "TraD/TraG TraM recognition site domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3D0UW40" ], "nt": "ATGATTTTGGTTTCCAAAATTTATAACTCAGCAATGGGAAGAGCCAAACTCGATCCTAAAGACAGAAAAGACTTTTATCTTTATGTGGATGAATTCCAAAACTTTGTATCGGGAACTTTTGCTGATATCCTTTCCGAAGCCAGAAAATATAGGCTTGGACTGATCATGGCGCACCAGTATATCGCTCAGCTCGAAGCAGGGAAAGGACTCTGA", "aa": "MILVSKIYNSAMGRAKLDPKDRKDFYLYVDEFQNFVSGTFADILSEARKYRLGLIMAHQYIAQLEAGKGL", "aa_hexdigest": "7e90f3ee670daa99f6667569be9a73e3", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3D0UW40", "query_cov": 1.0, "subject_cov": 0.9210526315789473, "identity": 0.857, "score": 120.0, "evalue": 1.47e-34, "valid": false, "product": "TraD/TraG TraM recognition site domain-containing protein", "uniref50_id": "UniRef50_A0A3D0UW40" }, "pscc": { "uniref50_id": "UniRef50_A0A3D0UW40", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3D0UW40" ], "product": "TraD/TraG TraM recognition site domain-containing protein" }, "genes": [ "traM" ], "id": "NKOCPLAKPK_556", "locus": "GCA003260355_00520" }, { "type": "cds", "sequence": "contig_1", "start": 274517, "stop": 275119, "strand": "-", "frame": 3, "gene": "herA", "product": "Helicase HerA central domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3D5YHS2", "UniRef:UniRef90_A0A3D5YHS2" ], "nt": "ATGGCAAGACAAGACCTCTGGAACGGAGACGGAATCTGCCTTATCGATCCGCACGGTGACCTTGCAGAAGACGTGCTAAGTTTCGTTCCAAAAGAAAGAGCTAAAGACGTAGTATATTTCGATGCAGGAAACGAAGACCGTCCAATGGGGCTTAATCTCTACGAAATCAATACTCTTGATGAAGCAGATAGAACCGTAAACGATGCAACAGAAATCTTTCTAAAAATGTTTGGACCAGAAATTTTCGGACCAAGAATTCAGGAATATTTTAAATACGGAAGCCTCACAATTCTTGAAGACTTTGAAGACAGACCAACCCTGCTCGATGTCGTCAGACTTTTTACTGATGAAACCTATAGAGAAGTAAAAGTTGCAAAGGTCACCAATGCGGTCGTAAAAAATCGACGAGAAAGGACCTATAATGCCATGGGAGACAGAGAAAAGGCTGAAATTATCCCATATTTCTCTTCAAAATTCGTATCTTTCAATACCAATAGATTGATCAGAAATATCATCGGACAAACGAAATCCGCCTTCAACTTTGATGACGTGATGAATAATCAAAAGATTCTCCTCGTTAACCTCTCAAAAGGTAAAATCTGA", "aa": "MARQDLWNGDGICLIDPHGDLAEDVLSFVPKERAKDVVYFDAGNEDRPMGLNLYEINTLDEADRTVNDATEIFLKMFGPEIFGPRIQEYFKYGSLTILEDFEDRPTLLDVVRLFTDETYREVKVAKVTNAVVKNRRERTYNAMGDREKAEIIPYFSSKFVSFNTNRLIRNIIGQTKSAFNFDDVMNNQKILLVNLSKGKI", "aa_hexdigest": "76f25b9c33bc30381b83c711db55cc69", "start_type": "ATG", "rbs_motif": null, "genes": [ "herA" ], "truncated": "both", "pseudogene": { "start": 274466, "stop": 275419, "observations": { "mutation": [ 274495, 274519, 275143, 275380 ], "stop": [ 274495, 274519, 275143, 275380 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A3D5YHS2", "query_cov": 1.0, "subject_cov": 0.5012531328320802, "identity": 0.835, "score": 352.0, "evalue": 3.64e-119, "gene_start": 1, "gene_end": 200, "reference_start": 182, "reference_end": 381, "reference_sequence": "MFGLTSFFFSKHYFGVNELASLFHFPDNAYNRSPAISWMQYKVLPAPENLPVLKDFGGYIIDGKFAENYKNGSVSSILEEYPNHRAVGTKTEKQEKLTPVTEYTSEQLTGKEIVEKDGHKFVKEIGEKQIHGYKVFKDGVLLGANIYRNNYSPVYMKREDRTRHHYCIGKSGTGKSVFLQTMARQDIWNGDGICLIDPHGDLAEDVLAYIPKERAKDVIYFDAGNEERPMGLNLYEISNLDEADRVVNDATEIFLKMFGPEIFGPRLQEYFKYGSLTLLEDFDDRPTLLDVVRLFTDESYREYKCKKVTNATVRNWRDKTFASMGDREKQEIIPYLSAKFVSFNTNRLIRNIIGQTKSAFTFEDVMNNRKILIINLSKGRIGELNAQLLGMIIVSKIYT" }, "paralog": false, "identity": 0.7602523659305994, "score": 478.0, "evalue": 3.14e-171, "description": "Internal stop codon at 274495, 274519, 275143, 275380. Nonsense mutation around 274495, 274519, 275143, 275380.", "psc": { "uniref90_id": "UniRef90_A0A3D5YHS2", "product": "Helicase HerA central domain-containing protein", "uniref50_id": "UniRef50_A0A3D5YHS2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A3D5YHS2", "UniRef:UniRef50_A0A3D5YHS2" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3D5YHS2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3D5YHS2" ], "product": "Helicase HerA central domain-containing protein" } }, "id": "NKOCPLAKPK_557", "locus": "GCA003260355_00521" }, { "type": "cds", "sequence": "contig_1", "start": 275378, "stop": 275962, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTAAGAATGGTCAGAGCAAAAGAAGACTCGATGAATGCTATTGGTGAAGAGGCAGGAAATCCAACCTACAGAGCTGGAATCATTGTGATTAGTTCCTCAGATGTAAAAGGACAAGCCAGACAAAACCTGAAAGCTATGGAATCCGCATACAATGTTTACAACGACGAATACAGCAACGGTCTTGAAGACAATAACACCAAGCACGATATCTTCTCTGGTATCTTCCTTTACCTGTGGAAAATTGCAGTAAATTTTTACCTTACAGGCTTTTTCTACAAATACTCTTTTTTCTCGACCAATGAACTCACATCACTCTTCCATTTTGCAGATAGTATTTACAACCGCTCTCCTATCATCGAGTGGATGCAATATAAGGTCCTTCCTTCACCAGCAAATCTCCCACAATTCAAGGATGATGAATGGAATGGCAAGATAATGTCAGGTATCCTCGCAGAAAAGTACAAAAATGGAAACCTCTCCGAGATTCTGAAAGAATACAAAAACCACCGAGCCGTAGCATCAAGGACACTAGAAGAAGAAAAGCTGACTCCTCTCGCAGATTATCAGCTTGCTCACAAGTGA", "aa": "MVRMVRAKEDSMNAIGEEAGNPTYRAGIIVISSSDVKGQARQNLKAMESAYNVYNDEYSNGLEDNNTKHDIFSGIFLYLWKIAVNFYLTGFFYKYSFFSTNELTSLFHFADSIYNRSPIIEWMQYKVLPSPANLPQFKDDEWNGKIMSGILAEKYKNGNLSEILKEYKNHRAVASRTLEEEKLTPLADYQLAHK", "aa_hexdigest": "19298190545e45e045f24d6c48abf63a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 22354.991600000012, "isoelectric_point": 6.1061220169067365 }, "id": "NKOCPLAKPK_558", "locus": "GCA003260355_00522" }, { "type": "cds", "sequence": "contig_1", "start": 276008, "stop": 276289, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCATTAGAACCTAAAAGAGACCCCGTCTATACAATCAGAACTTTTAAACAAATGCCGGATGATCCAATCAATAACCTGATTGATACGATGGGAAAAATCTCTAGCTACGACACAATAACTGCGATGCTCGTCATCAAACCTGAAAGCTCAACCTTCAATGCCAAGAGACAGAAAGCAGCAGAAAGACTTTATAAAGGGCTTGATCTTTATTCGACGAAATGGCGACACCGAAAAAACCTACTCAACCCCTTCAAACTCATCAGTTTTCTAATCCAATGA", "aa": "MPLEPKRDPVYTIRTFKQMPDDPINNLIDTMGKISSYDTITAMLVIKPESSTFNAKRQKAAERLYKGLDLYSTKWRHRKNLLNPFKLISFLIQ", "aa_hexdigest": "eada968fdab58feed443c8ceba6459fa", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10902.65119999999, "isoelectric_point": 9.869674491882325 }, "id": "NKOCPLAKPK_559", "locus": "GCA003260355_00523" }, { "type": "cds", "sequence": "contig_1", "start": 276545, "stop": 276844, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAACAGCAGCACAAACAAGGATACAAACTGGAACTACACAATCAACCGCCACACAAGAACTACAAAAAGGACGAGAAGTTATCGAAAAAATTGGAATCTACCTCTGCTATGGATTGATCGCACTCCTCTGTATTGGCGTCATCATCGGTATCATCAAAAATACGCTCAAATACATCTATTCTTTCTACAATACCAGAAGGATGACTTTCATCAAGGTACTTTTGCCAAAATCAGATGGGAAAACTGATCGTGAACAAGAAAAAGAAATCGCTAAAGATATGAAAGAAAAAGTCTGA", "aa": "MQTAAQTRIQTGTTQSTATQELQKGREVIEKIGIYLCYGLIALLCIGVIIGIIKNTLKYIYSFYNTRRMTFIKVLLPKSDGKTDREQEKEIAKDMKEKV", "aa_hexdigest": "9e84193048924eb6443f079690834fe6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11291.168499999985, "isoelectric_point": 9.449340629577637 }, "id": "NKOCPLAKPK_560", "locus": "GCA003260355_00524" }, { "type": "cds", "sequence": "contig_1", "start": 277007, "stop": 277306, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAACAAAGTGAATGCATGCAACTCTTGAGAACACAATACCTCAAAGACACAAAACAAAAAGATAACCTTTCAAAGCTAACAAATCAAGCTTGGAGAGAAAAGAAATCTGAATGTTTGACAACAATCGGAGGTTTTCCTCAAATCAGATCAGTACTCAATTCGTTTGAAGAAAAAGAACATTATGCGCCAAATACAAATTCAAATAGCACTCCATGCCAAGAGAAGCTCATCACAACATTCAGAAAAGATTTTAGTCAAAAAAATAACCTTTCAAAACTAACAAACCAAGCTTGGTGA", "aa": "MQQSECMQLLRTQYLKDTKQKDNLSKLTNQAWREKKSECLTTIGGFPQIRSVLNSFEEKEHYAPNTNSNSTPCQEKLITTFRKDFSQKNNLSKLTNQAW", "aa_hexdigest": "e3627eeae08524c0dc8f4386dab1ed2e", "start_type": "ATG", "rbs_motif": "TTTA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11589.946799999982, "isoelectric_point": 9.27108554840088 }, "id": "NKOCPLAKPK_561", "locus": "GCA003260355_00525" }, { "type": "cds", "sequence": "contig_1", "start": 277472, "stop": 277756, "strand": "+", "frame": 2, "gene": null, "product": "AMMECR1 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MQ07", "UniRef:UniRef90_A0A660MQ07" ], "nt": "ATGCTTGTGATTGATCGTATAAAAAGTGAAGTTTCTCAATATGTTGCTCTTCAGAGTAAATCTGGGGTGTTTTTGTCAGCGTTTGGTAAAGATGGGAAACTTTTACTTTCTCATGGAGTCGTTAGAACCAATAGAACCTTAGAAATGATTTTGCAGAGTTTTTATACTGCCTTTTTGCAGAAGATAGAAGCTCAAGTAGCAACTGTGATCCTAGATATTGTTCAAGATGTGAGATTGCAGAATGATCCGAATGTTTTAGTGAAGCTTCCTCTTGATCAGTATTGA", "aa": "MLVIDRIKSEVSQYVALQSKSGVFLSAFGKDGKLLLSHGVVRTNRTLEMILQSFYTAFLQKIEAQVATVILDIVQDVRLQNDPNVLVKLPLDQY", "aa_hexdigest": "d688236611ebb04fde9e3bb817abe03d", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 277472, "stop": 277924, "observations": { "mutation": [ 277754, 277778, 277790 ], "stop": [ 277754, 277778, 277790 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A660MQ07", "query_cov": 1.0, "subject_cov": 0.6266666666666667, "identity": 0.9890000000000001, "score": 176.0, "evalue": 4.29e-55, "gene_start": 1, "gene_end": 94, "reference_start": 1, "reference_end": 94, "reference_sequence": "MLVIDRIKSEVSQYVALQSKTGVFLSAFGKDGKLLLSHGVVRTNRTLEMILQSFYTAFLQKIEAQVATVILDIVQDVRLQNDPNVLVKLPLDQYGLFLVQDEGLKSGVLLPNTKGIDSPQQAFSAIKQKYNLGSQVSVYIFTTKKVEISF" }, "paralog": false, "identity": 0.9733333333333334, "score": 274.0, "evalue": 3.33e-96, "description": "Internal stop codon at 277754, 277778, 277790. Nonsense mutation around 277754, 277778, 277790.", "psc": { "uniref90_id": "UniRef90_A0A660MQ07", "product": "AMMECR1 domain-containing protein", "uniref50_id": "UniRef50_A0A660MQ07", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A660MQ07", "UniRef:UniRef50_A0A660MQ07" ] }, "pscc": { "uniref50_id": "UniRef50_A0A660MQ07", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MQ07" ], "product": "AMMECR1 domain-containing protein" } }, "id": "NKOCPLAKPK_562", "locus": "GCA003260355_00526" }, { "type": "cds", "sequence": "contig_1", "start": 277996, "stop": 278484, "strand": "-", "frame": 1, "gene": null, "product": "SMODS and SLOG-associating 2TM effector domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EJK7", "UniRef:UniRef90_A0A328EJK7" ], "nt": "ATGCACTTTATTATTGACTTTCATAATAAATTAAGTATCATACCAAGGGTAATAAAAAAAAATTTCACTATGGAAAAAAATTCAAATAAAGAGCTATTTAAAGAGCTGAAAGAAACGAAGCATAAGCTCAAAATCGCAGGTTTCACAATCTCTATTATGTTTGGGATTGTAATCGTTCCCATGTTTATGAATCTCAAACCAAGCTACCTAGAGCTCGTCCTCCCTAGTCTAATCGGAATTTTAGGTCCGATATATCTCTGGGTAGAAAAAAAACAGCTCAATCAGTCGATCAAAGGAATCATCAATCTCTTGGATGAAGATTCAGGACTTCGGCGACAACTAAAAGAAGAGATGCAAGAGAAACAAGCAAGCCTCAAAAGAGCAAACAGAGAGTGCGACACGAGTTTCTTTACGCGTAAGATTACTGAGTACGAGAAAAGGATAGCCGCAAATAAGTATTGGCGAACAAAATTTCAGGGTCTCCTCTGA", "aa": "MHFIIDFHNKLSIIPRVIKKNFTMEKNSNKELFKELKETKHKLKIAGFTISIMFGIVIVPMFMNLKPSYLELVLPSLIGILGPIYLWVEKKQLNQSIKGIINLLDEDSGLRRQLKEEMQEKQASLKRANRECDTSFFTRKITEYEKRIAANKYWRTKFQGLL", "aa_hexdigest": "b4d482a4e114ad7aaf11756b4df4156b", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A328EJK7", "query_cov": 0.8580246913580247, "subject_cov": 0.972027972027972, "identity": 0.971, "score": 258.0, "evalue": 1.32e-86, "valid": true, "product": "SMODS and SLOG-associating 2TM effector domain-containing protein", "uniref50_id": "UniRef50_A0A328EJK7" }, "pscc": { "uniref50_id": "UniRef50_A0A328EJK7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EJK7" ], "product": "SMODS and SLOG-associating 2TM effector domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_563", "locus": "GCA003260355_00527" }, { "type": "cds", "sequence": "contig_1", "start": 278608, "stop": 278787, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGCGAGAATCCCCGTCATTGAACAGTCAGTTTTTCCTCTCCTTCTTGCCATTCAAAGGAAGCTTCCTCAATTTGTGGCATCGGTAACTTACTCGTTTGAGCAAAATCTTGAAGCAGAGAAAATTCATAAGATAAAGCCTGATTTTTACTCTTATCTAAACAAGCAGCAAGAATAA", "aa": "MKARIPVIEQSVFPLLLAIQRKLPQFVASVTYSFEQNLEAEKIHKIKPDFYSYLNKQQE", "aa_hexdigest": "3ac83aebf8fe9c68f9aba6fcb013dbc7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6967.051499999998, "isoelectric_point": 9.09998645782471 }, "id": "NKOCPLAKPK_564", "locus": "GCA003260355_00528" }, { "type": "cds", "sequence": "contig_1", "start": 278883, "stop": 279419, "strand": "+", "frame": 3, "gene": null, "product": "Lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EG93", "UniRef:UniRef90_A0A328EG93" ], "nt": "ATGGGATTTGAAAAAGATGGGACGATTTGTCTTAGTGAAAGTCATATTCATGCCTCGCTCCAGCAAATCAATGATGATGCTCATTTCCCACAGCATGTGCCTTATAGCCTTCGTCTCACTTGTGGACAGAAAAGAGATCTCCAGATAACTACTAAACAAAAAGAAAAAAAATCTGAAAACACGACAAATCTTTGCCTTGCAGAGATTGAAAAGCAGATTTTTGCTTCACCTCAATTCATAGGAGTTAAACAAGACTATGATAAGGCTCTGGCTGCTCCGAATCAGGGTGATACCGAGCAGTTTTTGGGCTATTATCTAGATTTGGAAAGTATAGATGATGAGAGTTATAAGCTTGCTTTAGCTGAAAATTATCCGAGCAGAGTGCTGAAATATCATCGTTTTCAGGTAGATAAAAAGACAGGAGAAATCAGACTTTATGATGTTGCTTCAGATACCTATGATGAGATTATTCCTCACGATGAAAGGTATCCAGAGTTCTTTGCTCAGCACTGTAAAAATTTAGATGATAATAACTAA", "aa": "MGFEKDGTICLSESHIHASLQQINDDAHFPQHVPYSLRLTCGQKRDLQITTKQKEKKSENTTNLCLAEIEKQIFASPQFIGVKQDYDKALAAPNQGDTEQFLGYYLDLESIDDESYKLALAENYPSRVLKYHRFQVDKKTGEIRLYDVASDTYDEIIPHDERYPEFFAQHCKNLDDNN", "aa_hexdigest": "b83a8ae46443c17712c431cc479c90e2", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 278586, "stop": 279419, "observations": { "mutation": [ 278766, 278787 ], "stop": [ 278766, 278787 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EG93", "query_cov": 1.0, "subject_cov": 0.624561403508772, "identity": 1.0, "score": 362.0, "evalue": 3.54e-125, "gene_start": 1, "gene_end": 178, "reference_start": 108, "reference_end": 285, "reference_sequence": "MMKRLGMGVLLLGISFILAACLDKSKNQALSYEFSLLQDFAQTSKLPMPQIEEASFEWQEGEEKLTVQGRGFSLSGLQENPDEAVETFFHTWKKNEENSLGRGSVNVMGFEKDGTICLSESHIHASLQQINDDAHFPQHVPYSLRLTCGQKRDLQITTKQKEKKSENTTNLCLAEIEKQIFASPQFIGVKQDYDKALAAPNQGDTEQFLGYYLDLESIDDESYKLALAENYPSRVLKYHRFQVDKKTGEIRLYDVASDTYDEIIPHDERYPEFFAQHCKNLDDNN" }, "paralog": false, "identity": 0.9927797833935018, "score": 557.0, "evalue": 2.04e-204, "description": "Internal stop codon at 278766, 278787. Nonsense mutation around 278766, 278787.", "psc": { "uniref90_id": "UniRef90_A0A328EG93", "product": "Lipoprotein", "uniref50_id": "UniRef50_A0A328EG93", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EG93", "UniRef:UniRef50_A0A328EG93" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EG93", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EG93" ], "product": "Lipoprotein" } }, "id": "NKOCPLAKPK_565", "locus": "GCA003260355_00529" }, { "type": "cds", "sequence": "contig_1", "start": 281645, "stop": 281761, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAACTCTATAAAGCTAAAGGGTATGATGAGTCATTAAAAAGGCTAGATACCCTTATGCATGATCATGAAAGTTTTTTGGAAAAATTTTCTGATGATGCTGGTGTGCATATTTGA", "aa": "MKLYKAKGYDESLKRLDTLMHDHESFLEKFSDDAGVHI", "aa_hexdigest": "9a81fd60a6f19065c32012cf07665221", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4469.0162, "isoelectric_point": 5.785891151428221 }, "id": "NKOCPLAKPK_566", "locus": "GCA003260355_00530" }, { "type": "cds", "sequence": "contig_1", "start": 282040, "stop": 282207, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGATTTGGAAATGGAGACAAGGTTTCTGCTTATGGATATATGGCTAGTCCTACAGGTTTTCGAGAGGGCATAAAAGAGTTTTTGTCTCTTTTTGAAGAAAAATCTGCTCGAACTACAGAAAAAGCTAATGAACTGTTAGAGACAATAGGAAAAAGGCAAAGTTAA", "aa": "MRFGNGDKVSAYGYMASPTGFREGIKEFLSLFEEKSARTTEKANELLETIGKRQS", "aa_hexdigest": "4de11fa435bea4ef5d42bd7f4ec3100b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6177.8863, "isoelectric_point": 8.065720176696775 }, "id": "NKOCPLAKPK_567", "locus": "GCA003260355_00531" }, { "type": "cds", "sequence": "contig_1", "start": 282316, "stop": 282642, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAACGATCAGGAATCAGTCTTATGCTCCTTTTTTTGGGGGCGACTTTATTTTCGGGGTGTAAGGAAACTCAACCTACTGAGCCGGAAGTCAAACCAGCTACAGCATCTCCTGCACAAGCATTTCTAGAGACGCTTGCACCAGTATTTGGCAAAACAGCTAAAGACATTAAACCCTTAGCCTTTAGCGGAGTGTGGATAGATGGAGAACTCAACGGTTATGTTCTTCGTAATGATGCTCTCGCTTCAACTGAAAATATCTGGGACAAGATCGAGCAGACTTTTGAGGGTTGGGTGGAAAAAATCCAAGCTGATGGCGTATGA", "aa": "MKKRSGISLMLLFLGATLFSGCKETQPTEPEVKPATASPAQAFLETLAPVFGKTAKDIKPLAFSGVWIDGELNGYVLRNDALASTENIWDKIEQTFEGWVEKIQADGV", "aa_hexdigest": "ce4468034fd827c877c7de52adab59bf", "start_type": "ATG", "rbs_motif": "TTTTTA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11774.375599999996, "isoelectric_point": 4.9019880294799805 }, "id": "NKOCPLAKPK_568", "locus": "GCA003260355_00532" }, { "type": "cds", "sequence": "contig_1", "start": 282661, "stop": 283131, "strand": "+", "frame": 1, "gene": null, "product": "DUF306 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7W4HL05", "UniRef:UniRef90_A0A328EG49" ], "nt": "TTGAATGATACTCTCATCTGTTCTGTCTCTACTAAGCTTAATTACGATATGGATGAGGTGATGAACCTGGTGTACTTAGGGGAAGAAAATGCTGCTAAAAAGCAAGAAGCGACTGAGAAGCTTGATAAGATTTATGATGAGAGCACGAGAACCATTGAGGTTCATTGTGGGGAGTTTACTGAAAATCAACCTTCTCCGCTTGAGTTTAATTTTGATCTCTTCGGAGAAGAGCCATTCTGGGATGCAAGCCTAAGAGTAGATTCGTTTACTTATACGCTTCCAAATAATGAGACACACGCATTTGAAACTGATAATACGTATATCGGCTATTGGGGAACGTCTGGAAAGAATCTGATTTTTTCTGGTGAAAAATATGATCTTGGTGTTATAGAAGGTGAACTCACTAAAGAAGCTTGTCTCGATACGACTGTTGGTGAGACGCATCCTTACGAAGCAAGAATTAGCTTCTGA", "aa": "MNDTLICSVSTKLNYDMDEVMNLVYLGEENAAKKQEATEKLDKIYDESTRTIEVHCGEFTENQPSPLEFNFDLFGEEPFWDASLRVDSFTYTLPNNETHAFETDNTYIGYWGTSGKNLIFSGEKYDLGVIEGELTKEACLDTTVGETHPYEARISF", "aa_hexdigest": "65ac50d33cb0d73dcd6b7c421d3d167d", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 282361, "stop": 283434, "observations": { "mutation": [ 282640, 283129, 283240 ], "stop": [ 282640, 283129, 283240 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EG49", "query_cov": 1.0, "subject_cov": 0.4020618556701031, "identity": 0.929, "score": 304.0, "evalue": 2.75e-101, "gene_start": 1, "gene_end": 156, "reference_start": 116, "reference_end": 271, "reference_sequence": "MKKRSGISLMLLFLGATLFSGCKETQPAEPEVKPATVSPAQAFLETLAPVFGKTAKDIKPLAFSGVWIDGELNGYLLRNDALVSTENIWDKIEQTFEGWVEKTQADGVGESYVEYLKDNLICSVYTKLNYDMDEVMKLVYLEEEDAAKKQEATEKLDKIYDESTRTIEVHCGEFTEKQPSPLEFNFDLFGEEPFWDASLRVDSLTYTLPNNETHAFETDNTYIGYWGTSGKNLIFSGEKYDLGVIEGELSKEACLDTTVGETHPYKARISFGDGTFEGCADKADDFLVPGRQGKLENLLKKINYKNYTGKLNPKTASYGGIQAEGNLVEVFLTQPGSEQTEYLVLGKTDKGRETYWQGTTPVDDATCEKYVNVPRLMDFGIFSSCPRG" }, "paralog": false, "identity": 0.9467787114845938, "score": 695.0, "evalue": 1.12e-257, "description": "Internal stop codon at 282640, 283129, 283240. Nonsense mutation around 282640, 283129, 283240.", "psc": { "uniref90_id": "UniRef90_A0A328EG49", "product": "DUF306 domain-containing protein", "uniref50_id": "UniRef50_A0A7W4HL05", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EG49", "UniRef:UniRef50_A0A7W4HL05" ] }, "pscc": { "uniref50_id": "UniRef50_A0A7W4HL05", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7W4HL05" ], "product": "Lipoprotein" } }, "id": "NKOCPLAKPK_569", "locus": "GCA003260355_00533" }, { "type": "cds", "sequence": "contig_1", "start": 283467, "stop": 283694, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EFR5", "UniRef:UniRef90_A0A328EFR5" ], "nt": "ATGTCCGAGAGGATAGGCGTTTTTGGATTTAAGGCTTTTGAAGAGGAGAGAGATCAAAAAGGATCTCACTTTGGCATAATGGCTATTTTGATAGCAAAAAGAGGAAAGAGCAACTTTTGCAGGATTTGCAGAGGGGGCTTTTTTCTTTTTAGTGGAAATGTCGGATGGGTAGTTGGGGAGATCTATTTTTGCTTTGCTCTCAACCTAAAAATGTGGGAAAAAAGCTGA", "aa": "MSERIGVFGFKAFEEERDQKGSHFGIMAILIAKRGKSNFCRICRGGFFLFSGNVGWVVGEIYFCFALNLKMWEKS", "aa_hexdigest": "32a990efa0d2eba8bcf27b2f80a2d471", "start_type": "ATG", "rbs_motif": "TTTTT", "psc": { "uniref90_id": "UniRef90_A0A328EFR5", "query_cov": 1.0, "subject_cov": 0.9493670886075949, "identity": 1.0, "score": 161.0, "evalue": 1.32e-50, "valid": true, "uniref50_id": "UniRef50_A0A328EFR5" }, "pscc": { "uniref50_id": "UniRef50_A0A328EFR5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EFR5" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8593.984099999998, "isoelectric_point": 9.255484199523924 }, "id": "NKOCPLAKPK_570", "locus": "GCA003260355_00534" }, { "type": "cds", "sequence": "contig_1", "start": 283732, "stop": 284055, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EI20", "UniRef:UniRef90_A0A328EI20" ], "nt": "ATGAAAGTGGTGTCAGACCTAGGGTGGATTTCTATCAAAAAGCTCAAATTACTCTCAGACCCAGGGTGGATTTCTATCAAAAAGCCCAAATTACTCTCAGACCCAGGGTGGATTTCTATCAAAAAGCTCAAATTACTCTCAGATCTAGGTGGATTTCTATCAAAAAGCTCAAATTACTCTCAGACTCAGGTGGATTTTCATCAAAAAGCTCAAATTACTCTTAGACCCAGGTGGATTTCTATCAAAAAGCTCAAATTACTCTCGGACCTAGGTGGATTTCTATCAAAAAGTCGAAAGTGTGGTAGGAGGATCATAGGTTCCTGA", "aa": "MKVVSDLGWISIKKLKLLSDPGWISIKKPKLLSDPGWISIKKLKLLSDLGGFLSKSSNYSQTQVDFHQKAQITLRPRWISIKKLKLLSDLGGFLSKSRKCGRRIIGS", "aa_hexdigest": "3a436ca194a4dc5fc7e4abb0f5a921bd", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 283732, "stop": 284184, "observations": { "mutation": [ 284053, 284083, 284086, 284143 ], "stop": [ 284053, 284083, 284086, 284143 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EI20", "query_cov": 1.0, "subject_cov": 0.7133333333333334, "identity": 1.0, "score": 86.3, "evalue": 2.34e-19, "gene_start": 1, "gene_end": 107, "reference_start": 1, "reference_end": 107, "reference_sequence": "MKVVSDLGWISIKKLKLLSDPGWISIKKPKLLSDPGWISIKKLKLLSDLGGFLSKSSNYSQTQVDFHQKAQITLRPRWISIKKLKLLSDLGGFLSKSRKCGRRIIGSGFFYKKAIRNGGKSLQKFEMFRFFLLVGGKGANLPAHNECRTS" }, "paralog": false, "identity": 0.9666666666666667, "score": 156.0, "evalue": 1.31e-49, "description": "Internal stop codon at 284053, 284083, 284086, 284143. Nonsense mutation around 284053, 284083, 284086, 284143.", "psc": { "uniref90_id": "UniRef90_A0A328EI20", "uniref50_id": "UniRef50_A0A328EI20", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EI20", "UniRef:UniRef50_A0A328EI20" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EI20", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EI20" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 12050.279899999983, "isoelectric_point": 10.525704765319823 }, "id": "NKOCPLAKPK_571", "locus": "GCA003260355_00535" }, { "type": "cds", "sequence": "contig_1", "start": 284358, "stop": 284729, "strand": "+", "frame": 3, "gene": null, "product": "GIY-YIG domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3D5YEH0" ], "nt": "ATGCTCAAGCCTTCTGCGATTGCTCACTTTCCAAATACGCCTGGTGTTTACCTTTTCAAAGATAAGAAGGGGCAGATTCTCTATATCGGGAAAGCAAAGAATTTGAAGAAGAGGGTTGGGCAATACTTCACTCCAGGGAGTGTTTGGAAGCAGGATATGATTGCACAGGCGGAGACCGTGGATTTTCTCAGTGTGGAGAATGAGAGTGAAAGTCTCTATCTAGAATCTAATCTCATCAAGAAGCATTTGCCTCCGTTTAATAATATGCTGAAGGGGGCGAATGCCTATGCTTATATTAAGCTCACGAAGCATCCTGTTCCTCAGCTTTTCATCACGAGAAAGAAAGTAAATGACGGAGCGGTCTATATTTGA", "aa": "MLKPSAIAHFPNTPGVYLFKDKKGQILYIGKAKNLKKRVGQYFTPGSVWKQDMIAQAETVDFLSVENESESLYLESNLIKKHLPPFNNMLKGANAYAYIKLTKHPVPQLFITRKKVNDGAVYI", "aa_hexdigest": "12a7e37d34735cb9ead010e0c7032b7b", "start_type": "ATG", "rbs_motif": "TTCTT", "psc": { "uniref90_id": "UniRef90_A0A948C5Z3", "query_cov": 0.8780487804878049, "subject_cov": 1.0, "identity": 0.685, "score": 158.0, "evalue": 5.33e-48, "valid": false, "product": "GIY-YIG nuclease family protein", "uniref50_id": "UniRef50_A0A3D5YEH0" }, "pscc": { "uniref50_id": "UniRef50_A0A3D5YEH0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3D5YEH0" ], "product": "GIY-YIG domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_572", "locus": "GCA003260355_00536" }, { "type": "cds", "sequence": "contig_1", "start": 284907, "stop": 285851, "strand": "+", "frame": 3, "gene": "uvrC", "product": "excinuclease ABC subunit UvrC", "db_xrefs": [ "GO:0006289", "GO:0009380", "GO:0009381", "KEGG:K03703", "SO:0001217", "UniRef:UniRef50_A0A328EGD2", "UniRef:UniRef90_A0A328EGD2" ], "nt": "TTGATTACGAGTTTTTTTAAAGGAAACACTCAGCCCATAGAAAAACAGCTTAAAAAGCTGATTGATGATGCTGTAGCGGTGCAGAATTTTGAGTGGGCGGCAAAATTGAGGGATATTTATTTACAAATTTCGCAGTTTACTGAGAAACAATCTGTTGAATATGCGAAATCTTTTTCTGGTTATTTATTGCAGATTCGTCAAATTGGGTTGTGGAGGTGTTATGTGTTGTTGCATTTTTTTGAAGGGAAGATGATCGATGTGATCCGTCATCACTTTCAAGAAGAAGATTTCGATACCGACGAAATGTTGGCGAATTTTTCTTCTGAGTTTGGGACGTTTAGTCCGCAAGGAAATTGGTTTGCTACGGAGAAGTTTTCTCTTACCAAAGAAGAAACTGCACGCATGGATCAGCTTTTTGATGACTTTTTTCAATCCTATCTTTTGGCACAGACTTTGCAAGGAGGGAGTGTGATGAAGGAGCTGTTGACTCAGCTTCAGCAGCGTTATCATTTATCGCATTTCCCGTATCAGATTGAATGTTTGGATATTTCTCATTTAAGTGGGGAGCGGACGAGCGGAGGATTGAGCTGTTTGGTTGGCGGGCTAGAAGAGAAAAGGAACTACAGGAAATACAAAATTCAGACTGTGAAAAATGATGATTATCAGGCATTACAGGAAGTTTTGGTCAGGAGATTTGGGTTGGCGGAGAAACAAGAGGTGGCGAAGTTTGTACTACCGAATCTTTTTATTCTCGACGGAGGGAAAGGTCAGCTTTCAATTCTTTTTGACTTATTAAAGCAATATCCTGCGATAGAAAAGCTTTTTTCAGAAGTGCAATTTGCAGCGCTTGGGAAGGGAGAAGCGAGGAAGAAGTCCCATATTGGGCAAAAGAGTAAAAAGTCTGATTCCACGGTAGGAGAGAAGTTGTATGTCTGGAAGCATTGA", "aa": "MITSFFKGNTQPIEKQLKKLIDDAVAVQNFEWAAKLRDIYLQISQFTEKQSVEYAKSFSGYLLQIRQIGLWRCYVLLHFFEGKMIDVIRHHFQEEDFDTDEMLANFSSEFGTFSPQGNWFATEKFSLTKEETARMDQLFDDFFQSYLLAQTLQGGSVMKELLTQLQQRYHLSHFPYQIECLDISHLSGERTSGGLSCLVGGLEEKRNYRKYKIQTVKNDDYQALQEVLVRRFGLAEKQEVAKFVLPNLFILDGGKGQLSILFDLLKQYPAIEKLFSEVQFAALGKGEARKKSHIGQKSKKSDSTVGEKLYVWKH", "aa_hexdigest": "ff48f8db0d152b8bb6d434da42a1a521", "start_type": "TTG", "rbs_motif": null, "genes": [ "uvrC" ], "truncated": "both", "pseudogene": { "start": 284607, "stop": 285992, "observations": { "mutation": [ 284727, 284850, 285849 ], "stop": [ 284727, 284850, 285849 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EGD2", "query_cov": 1.0, "subject_cov": 0.5772058823529411, "identity": 0.997, "score": 625.0, "evalue": 6.66e-223, "gene_start": 1, "gene_end": 314, "reference_start": 184, "reference_end": 497, "reference_sequence": "MLKPSAIAHFPNTPGVYLFKDKKGQILYIGKAKNLKKRVGQYFTPGSVWKQDMIAQAETVDFLSVENESESLYLESNLIKKHLPPFNNMLKGANAYAYIKLTKHPVPQLFITRKKVNDGAVYIGPKHNTRELKKFLQYLRQIVQYRTCPLSQFKEGKLCSDFYFGLCKGWCAKPELEKPDYPQLITSFFKGNTQPIEKQLKKLIDDAVAVQNFEWAAKLRDIYLQISQFTEKQSVEYAKSFSGYLLQIRQIGLWRCYVLLHFFEGKMIDVIRHHFQEEDFDTDEMLANFSSEFGTFSPQGNWFATEKFSLTKEETARMDQLFDDFFQSYLLAQTLQGGSVMKELLTQLQQRYHLSHFPYQIECLDISHLSGERTSGGLSCLVGGLEEKRNYRKYKIQTVKNDDYQALQEVLVRRFGLAEKQEVAKFVLPNLFILDGGKGQLSILFDLLKQYPAIEKLFSEVQFAALGKGEARKKSHIGQKSKKSDSTVGEKLYVWKHGSIEEFDLVYDEADRLLTKVRDEAHRFANYYRKQQERLAFKKAQEGL" }, "paralog": false, "identity": 0.9934924078091106, "score": 925.0, "evalue": 0.0, "description": "Internal stop codon at 284727, 284850, 285849. Nonsense mutation around 284727, 284850, 285849.", "psc": { "uniref90_id": "UniRef90_A0A328EGD2", "gene": "uvrC", "product": "excinuclease ABC subunit UvrC", "uniref50_id": "UniRef50_A0A328EGD2", "kegg_orthology_id": "K03703", "go_ids": [ "GO:0006289", "GO:0009380", "GO:0009381" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EGD2", "UniRef:UniRef50_A0A328EGD2", "KEGG:K03703", "GO:0006289", "GO:0009380", "GO:0009381" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EGD2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EGD2" ], "product": "Excinuclease ABC subunit C" } }, "id": "NKOCPLAKPK_573", "locus": "GCA003260355_00537" }, { "type": "cds", "sequence": "contig_1", "start": 285873, "stop": 285992, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTATGATGAGGCAGATCGTTTGCTGACGAAGGTTAGAGATGAGGCTCATAGATTTGCGAACTATTATCGTAAGCAGCAAGAAAGGTTGGCATTTAAAAAAGCCCAGGAAGGGCTTTAG", "aa": "MYDEADRLLTKVRDEAHRFANYYRKQQERLAFKKAQEGL", "aa_hexdigest": "6efef5dbfe354449f0fff32ac09c3836", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4789.349999999999, "isoelectric_point": 9.220993614196775 }, "id": "NKOCPLAKPK_574", "locus": "GCA003260355_00538" }, { "type": "cds", "sequence": "contig_1", "start": 286169, "stop": 286264, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATATTGAATGAGATGAATTTTTTGTATTTCATTTCTCCGATCCCGGCAACGAAAGGAGCAAAAGTTCTGATGATGGGGATGAATCTAGCGTAG", "aa": "MILNEMNFLYFISPIPATKGAKVLMMGMNLA", "aa_hexdigest": "9f892ea5a3ff2b0490a91547223a4f8f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3460.2646000000004, "isoelectric_point": 8.254290199279787 }, "id": "NKOCPLAKPK_575", "locus": "GCA003260355_00539" }, { "type": "cds", "sequence": "contig_1", "start": 286360, "stop": 286662, "strand": "-", "frame": 1, "gene": "dedA", "product": "DedA family protein", "db_xrefs": [ "KEGG:K03975", "SO:0001217", "UniRef:UniRef50_A0A328EJC6", "UniRef:UniRef90_A0A328EJC6" ], "nt": "ATGAACACGCTCCTTTCCGCGATCAAATACCTCTTCAACTTCATCATCCACATCGATAAACACCTCGTTGAGCTGTTTAATAGTTATGGAATACGAATCTACGGAATCATTTTCCTGATTATCTTCATCGAGACCGGATTAGTTGTAATGCCATTCCTGCCTGGAGATTCTTTATTGTTTGCTGCTGGTGCCTTAGCAGGCAACCCAGAAAACACGATCAATGTTCATCTAATTCGATTGATCGTCTTCTTTGCGGCCATTATCGGTGATAGCGTAAACTACGAAATCGGTAAATTCCTCTGA", "aa": "MNTLLSAIKYLFNFIIHIDKHLVELFNSYGIRIYGIIFLIIFIETGLVVMPFLPGDSLLFAAGALAGNPENTINVHLIRLIVFFAAIIGDSVNYEIGKFL", "aa_hexdigest": "962d2698d0c97c27716b536e39fdfda3", "start_type": "ATG", "rbs_motif": "TTCTT", "psc": { "uniref90_id": "UniRef90_A0A376RFM6", "query_cov": 0.94, "subject_cov": 0.8245614035087719, "identity": 0.574, "score": 112.0, "evalue": 4.78e-30, "valid": false, "product": "Inner membrane protein", "uniref50_id": "UniRef50_A0A0G0ERC6", "kegg_orthology_id": "K03975" }, "genes": [ "dedA" ], "truncated": "5-prime", "pseudogene": { "start": 286057, "stop": 286662, "observations": { "mutation": [ 286119, 286362 ], "stop": [ 286119, 286362 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EJC6", "query_cov": 1.0, "subject_cov": 0.4329004329004329, "identity": 1.0, "score": 193.0, "evalue": 1.43e-60, "gene_start": 1, "gene_end": 100, "reference_start": 1, "reference_end": 100, "reference_sequence": "MNTLLSAIKYLFNFIIHIDKHLVELFNSYGIRIYGIIFLIIFIETGLVVMPFLPGDSLLFAAGALAGNPENTINVHLIRLIVFFAAIIGDSVNYEIGKFLGPKVFTGKIKRIKKEHLEKTQHFYDKHGGKTIIYARFIPIIRTFAPFVAGIGEMKYKKFISFNIIGGFLRSALFIYAGYSFGSLEIVQKRFTLIIFAIILLSLAPILIQRISHKIKSKKKQITPKALPGLF" }, "paralog": false, "identity": 0.9900497512437811, "score": 389.0, "evalue": 2.39e-140, "description": "Internal stop codon at 286119, 286362. Nonsense mutation around 286119, 286362.", "psc": { "uniref90_id": "UniRef90_A0A328EJC6", "product": "DedA family protein", "uniref50_id": "UniRef50_A0A328EJC6", "kegg_orthology_id": "K03975", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EJC6", "UniRef:UniRef50_A0A328EJC6", "KEGG:K03975" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EJC6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EJC6" ], "product": "VTT domain-containing protein" } }, "id": "NKOCPLAKPK_576", "locus": "GCA003260355_00540" }, { "type": "cds", "sequence": "contig_1", "start": 288460, "stop": 288675, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTGTATTGTTGTATCGATTGATTTGGAGTGCTTTGAAATCTTGTGGAATCGCAATCCTGGCAATTTTTTTTACTTTTGTGAGATCGACGAGTGGAGAGTTGAAGCTTTTTGGTCATTGGATGACCGTTAAAATCTGTGTTTTTTCGTCGAATGTGGTCAAAAAAGTATTTGATTCAGATTTTTCTGTTCCTAGATCGATAGTTGAGACTTGA", "aa": "MSVLLYRLIWSALKSCGIAILAIFFTFVRSTSGELKLFGHWMTVKICVFSSNVVKKVFDSDFSVPRSIVET", "aa_hexdigest": "9645270e8ac8657eaaa86d2e6ecff2f5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8021.485199999999, "isoelectric_point": 9.509683036804201 }, "id": "NKOCPLAKPK_577", "locus": "GCA003260355_00541" }, { "type": "cds", "sequence": "contig_1", "start": 288576, "stop": 288860, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATAAAAAAAATATCTTTTCTCTTAGGGGTTTTGATCTGCGGAGTTTTCACCAAAGCAAACGCTAATGAGAAGCCAGAAACCGATCGAAAACCTGTAAAATCATGTATCGATCTCAGTAATCAATTCGAGAAATTGGTCAAAAACGGTACTCCTCTTCCCTCTCCACTGTATACAGAAAGTCATCAAGTCTCAACTATCGATCTAGGAACAGAAAAATCTGAATCAAATACTTTTTTGACCACATTCGACGAAAAAACACAGATTTTAACGGTCATCCAATGA", "aa": "MIKKISFLLGVLICGVFTKANANEKPETDRKPVKSCIDLSNQFEKLVKNGTPLPSPLYTESHQVSTIDLGTEKSESNTFLTTFDEKTQILTVIQ", "aa_hexdigest": "4199c7bf21803fce3595f4852d436297", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10459.928199999991, "isoelectric_point": 6.557878303527832 }, "id": "NKOCPLAKPK_578", "locus": "GCA003260355_00542" }, { "type": "cds", "sequence": "contig_1", "start": 288957, "stop": 289223, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTCAATATCTTTCTAGAAGTTGAAGCAAGAGTAGATGAAAATGGAGAAATCAGTTACACTAAAAGTGTTGTAAAAGGGATTGAGCAAAAAGGAATAATGACTTCTGAAAGGATGAAGCTTTACCGCGGTCGAGAGAATCTCAAAACCTTTGACCTTAGAGTCTCAGAAGAGCAAGACAGCAATCCATCGATTGAACAAAGAATAGAAACACTTGAAAACAGAATCAAAAACTTGGAACAGCAAATCAGCGAAACATACAATTAA", "aa": "MFNIFLEVEARVDENGEISYTKSVVKGIEQKGIMTSERMKLYRGRENLKTFDLRVSEEQDSNPSIEQRIETLENRIKNLEQQISETYN", "aa_hexdigest": "fe89ebe0cf2bc815b74440d6cd54e060", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10388.520699999994, "isoelectric_point": 4.910286521911621 }, "id": "NKOCPLAKPK_579", "locus": "GCA003260355_00543" }, { "type": "cds", "sequence": "contig_1", "start": 289332, "stop": 289943, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTCTGAATTCACAAGGGAACTTTATATTCCAATCCCTGAAAAAAATCAACCTTTAACTATTAGTATAGAACCAAATAATCTCATCCTACCAATAGACCCATTTCAAGATTTTATAATAGAAAATAGTAGTATTAATTACATTCCTTACGAAGGAACCAAAATCAAGGCAGTTTTCAAGTATCAAATTAATGCTTACAATAAAGAAACTTTCGCTAAAATAACAGAAAACCTTTCCGAACTTATCTATGAAATCAAAGACGACGTGCTTCATATTTCCCACAAAAATCACCAACTTTTTGATGCAGTAGTGCCTCTTGCTCCTATGATCTATTCTGTAGATCTCTACATTCCCAAAAATCGAAAATTCAACACCAAAAACCCAGTTTATAGGACCAACCTTCACAAACCAGAACGAATGAACGAACGAGGATATTCTGAATATGATTATGATCCGAATGCAAACAAGTGTTCTGAATTAATCTCTTATGTTGAAGAAGAAAATGCCTTTTTCTGCCCTATCAAATTTAACCTCGACACCTGGAATATCAGAAACAGAATCAATGATGATATTAGAGATAATAAAGCTGACAAACTTTCTCCTCTAGAATGA", "aa": "MSEFTRELYIPIPEKNQPLTISIEPNNLILPIDPFQDFIIENSSINYIPYEGTKIKAVFKYQINAYNKETFAKITENLSELIYEIKDDVLHISHKNHQLFDAVVPLAPMIYSVDLYIPKNRKFNTKNPVYRTNLHKPERMNERGYSEYDYDPNANKCSELISYVEEENAFFCPIKFNLDTWNIRNRINDDIRDNKADKLSPLE", "aa_hexdigest": "1eeedbb078145b832446cd1ea7952f3e", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 23988.891500000023, "isoelectric_point": 5.229437446594236 }, "id": "NKOCPLAKPK_580", "locus": "GCA003260355_00544" }, { "type": "cds", "sequence": "contig_1", "start": 290017, "stop": 290193, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTGCAGTGCTTAAAGTGGCTGTATTCATAAGCCTCTTCTTTGATAACGCACCGATACTTCCTAACAACAGTAATAATGCTGATAGAAATCCAAAAGCTATTCCTAGGGAAGTAATTGCTGGGAATCGGGAAGTTATCTCCATATTATTCCATACATATCAAGTTTGTAGGTAA", "aa": "MIAVLKVAVFISLFFDNAPILPNNSNNADRNPKAIPREVIAGNREVISILFHTYQVCR", "aa_hexdigest": "849542127fbd69fc46fc9dc15309b541", "start_type": "ATG", "rbs_motif": "TTATT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6511.5344000000005, "isoelectric_point": 9.183408546447755 }, "id": "NKOCPLAKPK_581", "locus": "GCA003260355_00545" }, { "type": "cds", "sequence": "contig_1", "start": 290200, "stop": 290403, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTCCGAGTAGGCCAAGGACAAAGACAAGAAATATACTTCGGAAAAAGAGGAAACAGATTTTTATGAAACTCCAAAAAGGTCAAAAGAGTTTTGCTCCTCGTTTTAAGAGCGTAGGAATAGCCGTTCATACAATTCGTTTCACGCTTCTGAATCAGAATTTTATACTATTGCTAATGAGGAGCCTCAAATCCCAAATTTGA", "aa": "MIPSRPRTKTRNILRKKRKQIFMKLQKGQKSFAPRFKSVGIAVHTIRFTLLNQNFILLLMRSLKSQI", "aa_hexdigest": "aa03da0f0bc4d0ceffcb4727bbd86585", "start_type": "ATG", "rbs_motif": "TTGTA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7953.633799999996, "isoelectric_point": 11.999967765808105 }, "id": "NKOCPLAKPK_582", "locus": "GCA003260355_00546" }, { "type": "cds", "sequence": "contig_1", "start": 290355, "stop": 290597, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTGCACAAATAAGTGGACGAAACGTGTGGATTTGGAGAGGATTAACGCTATTTGCAAGCTATGTATTTCTCACAAGCGGAACAGGAATTCTCTTTTTCTGGGGTGTTATGGCTTATCTTTTGCTTGCTCTTATCTTTCCAATCACAGAGAAAAACTATGGGGAAAGATCAGTTTTTTCTTACTTTTTGACTCAAATTTGGGATTTGAGGCTCCTCATTAGCAATAGTATAAAATTCTGA", "aa": "MVAQISGRNVWIWRGLTLFASYVFLTSGTGILFFWGVMAYLLLALIFPITEKNYGERSVFSYFLTQIWDLRLLISNSIKF", "aa_hexdigest": "5bb3a0d9a695849e0e34466a11d3f86b", "start_type": "ATG", "rbs_motif": "TTCTT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9305.901299999992, "isoelectric_point": 9.693224525451662 }, "id": "NKOCPLAKPK_583", "locus": "GCA003260355_00547" }, { "type": "cds", "sequence": "contig_1", "start": 290805, "stop": 290990, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAACACTCAATCTCAATGAAAAATCATTCCAACTTGAAAATAATGTTTTCAAGTTCCTGCAGGATTATATAGAGAGAATCAATACTTTCGTAGCCAAGAATGAAATTGATACGGACCTTCATCAAGATATTTTGCAGAGACTTGCAGACAAGCTCGCCGAAAAAGCAACAGAAAAAACCTGA", "aa": "MKTLNLNEKSFQLENNVFKFLQDYIERINTFVAKNEIDTDLHQDILQRLADKLAEKATEKT", "aa_hexdigest": "d1bae2ac560c97ceba150809f08b1625", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7228.111500000001, "isoelectric_point": 5.236087608337402 }, "id": "NKOCPLAKPK_584", "locus": "GCA003260355_00548" }, { "type": "cds", "sequence": "contig_1", "start": 291118, "stop": 291438, "strand": "-", "frame": 1, "gene": "padR", "product": "PadR family transcriptional regulator", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MWD6", "UniRef:UniRef90_A0A328EGB6" ], "nt": "ATGCTGATGTATGATACCGATAATATTGCAATCCAGATGAGAAAAGGAATTTTAGAATATCTTATTCTCCTCATTATTAGTAAAGGTGAGGTCTATGCTTCAGACATTATCAGCGGATTACAAAAATATGATCTCCTCATCGTAGAAGGGACGCTCTATCCACTCCTCTCTCGCCTCAAAAAAGATCAGCTTTTAACCTATTCCTGGGTAGAGTCAGTCAGTGGCCCTCCAAGAAAATATTATAAACTGACTCCTCTCGGCAAACAGGTTATGAAAAAGATGGAACAAATCCGAAATCAGCTCAATACCGCCATTCTCTGA", "aa": "MLMYDTDNIAIQMRKGILEYLILLIISKGEVYASDIISGLQKYDLLIVEGTLYPLLSRLKKDQLLTYSWVESVSGPPRKYYKLTPLGKQVMKKMEQIRNQLNTAIL", "aa_hexdigest": "30a90ee44820ea9332a21de34c616981", "start_type": "ATG", "rbs_motif": "TTTA", "psc": { "uniref90_id": "UniRef90_A0A328EGB6", "query_cov": 0.9811320754716981, "subject_cov": 0.9541284403669725, "identity": 0.99, "score": 197.0, "evalue": 1.11e-63, "valid": true, "product": "PadR family transcriptional regulator", "uniref50_id": "UniRef50_A0A660MWD6" }, "pscc": { "uniref50_id": "UniRef50_A0A660MWD6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MWD6" ], "product": "PadR family transcriptional regulator" }, "genes": [ "padR" ], "id": "NKOCPLAKPK_585", "locus": "GCA003260355_00549" }, { "type": "cds", "sequence": "contig_1", "start": 291563, "stop": 292165, "strand": "-", "frame": 3, "gene": null, "product": "Glycerol-3-phosphate dehydrogenase [NAD(P)+]", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EJN7", "UniRef:UniRef90_A0A328EJN7" ], "nt": "GTGCCGATTATCGTGCTTTCAGGCGCCTCTCACGCCGAAGAGGTCGTAAAACACCAGCCAACCAAAGTGGTCTTGGCTTCATCCAACAAAGAACGAAGAGAAAAGATTAAAAATCTGTTTCACAACAAGCGGTTTAAATGTGAAGAAAGCGAAGATATCGTCGGAGTACAGCTTATGGGAGCATTCAAAAATATCATTGCACTTGCTTGCGGGATCTCTGATGGTCTCAAATACGGCACAAATACCAAAGCACTCATTTTGACAGAGGGAATCCAAGAAATCAGCAAAATCGGCTCCTCTCTAAAAATCAAACCCGAAACTCGGAAAAGTTCTGCGGGGATCGGAGATCTCTATGTTACATGCTCAAGCGTATTAAGCAGAAACCGAAACGCTGGACGCCTACTAGCAGAAGGACATAGTAAAGCAGAAATTACAGGAGGCTTGATAAATATGGTTGCAGAAAGCCTCTATTTAACAGAAGCTATTGCCAAATTTCTCGCAACTACGAACGAAGCATTCCCGCTATTAGACATGATTATCAAGATTGTCGAATACAATACCCCAGCCCAAAAAGCTTTTGATCTATTCCTAGAAAGCTCTTAA", "aa": "MPIIVLSGASHAEEVVKHQPTKVVLASSNKERREKIKNLFHNKRFKCEESEDIVGVQLMGAFKNIIALACGISDGLKYGTNTKALILTEGIQEISKIGSSLKIKPETRKSSAGIGDLYVTCSSVLSRNRNAGRLLAEGHSKAEITGGLINMVAESLYLTEAIAKFLATTNEAFPLLDMIIKIVEYNTPAQKAFDLFLESS", "aa_hexdigest": "594fd1aa88d3528cb534d08518011be0", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 291563, "stop": 292465, "observations": { "mutation": [ 292252, 292387 ], "stop": [ 292252, 292387 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EJN7", "query_cov": 1.0, "subject_cov": 0.6172839506172839, "identity": 0.95, "score": 360.0, "evalue": 1.35e-123, "gene_start": 1, "gene_end": 200, "reference_start": 125, "reference_end": 324, "reference_sequence": "MKCTIIGAGSWGTALGQVLSENGHETKLFSLSQADADSINQKHENFEYLPGVQLHEKLTCSTNLAQTLDGAEIIVLAVPSKGLTSCLRELKPYYTGQPIVLASKGWNASDFSPLSSLITEKLGAVPIIVLSGASHAEEVVKHQPTKVVLASSDKERREKIKNLFHNKRFKCEESEDIVGVQLMGAFKNIIALACGISDGLKYGTNTKALILTEGIQEISKIGSSLKIQPETRKSSAGIGDLYVTCSSVLSRNRNAGRLLAEGHSKAEITGGLINMVAESLYLTEAIEKFLTTTTHQFPLLEMIIKIVEYNIPAQKAFDLFLESS" }, "paralog": false, "identity": 0.95, "score": 550.0, "evalue": 1.57e-200, "description": "Internal stop codon at 292252, 292387. Nonsense mutation around 292252, 292387.", "psc": { "uniref90_id": "UniRef90_A0A328EJN7", "product": "Glycerol-3-phosphate dehydrogenase [NAD(P)+]", "uniref50_id": "UniRef50_A0A328EJN7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EJN7", "UniRef:UniRef50_A0A328EJN7" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EJN7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EJN7" ], "product": "Glycerol-3-phosphate dehydrogenase [NAD(P)+]" } }, "id": "NKOCPLAKPK_586", "locus": "GCA003260355_00550" }, { "type": "cds", "sequence": "contig_1", "start": 292600, "stop": 293376, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EG59", "UniRef:UniRef90_A0A328EG59" ], "nt": "ATGAAAATAGGAATCATCTCAAGTGGAATGGAAAGCTTGGCACTCTTCAGCTTCTTGCACCGCCACGACCAAGAATACCTCATCTACTATGATAGTCTCAACGCCCCTTATGGAGAGAAACACTTTGAAACCTCCTTACAAGCCGTCAGAAAAGGAATCGAGCGACTAAAAAATAAAGAAGTAGACCTCATAATTCTACCACCTATATATGAACTCTATCTCCTACAAGAAAAAAAAGAAAAGCTGATTGCTCCCCTCTTTCAGACTTACCTTCTCACAGAAGTTTTCGCTCACTCTCTCGTCGGAAAATTAGGAATTCTTGGAGAATATGCTGACTTAGAAAAAGCCCAATCCTTACTAACTGAGCTCTCCAGCTCCTATACTCCGACTCCCGCACAAAAAACCACAAAAACTTTTCACTTTCCCTTCCAGTACCGATCCAAAGAATGCGGCATCCTCAATCCGCTCCTTAGCAGACTCTCCCGAAAAAGCATTCTTACTAATACACTTTTAAAGCACGAGCTCCGCTATTTCAAGGATGCAGCAGTCGATTCACTCATTCCGCTCAACTACCTCTATTTCAACGCAGAAAAAACCATCGCTAAATTCCTAAATTTCAGAAAAATCAGATTCCACCGCCAAGAGAAGCTCGAAAAGATCTTCCTCGAACTGACAAAAAAGAAAGAAAAATCAGAATACTCCATTTCAATTTTCGCCACCGACCAGAACCATTCCTTACTCAGAGAGAAAAGACTCCTCCGATACTTACAAAGATGA", "aa": "MKIGIISSGMESLALFSFLHRHDQEYLIYYDSLNAPYGEKHFETSLQAVRKGIERLKNKEVDLIILPPIYELYLLQEKKEKLIAPLFQTYLLTEVFAHSLVGKLGILGEYADLEKAQSLLTELSSSYTPTPAQKTTKTFHFPFQYRSKECGILNPLLSRLSRKSILTNTLLKHELRYFKDAAVDSLIPLNYLYFNAEKTIAKFLNFRKIRFHRQEKLEKIFLELTKKKEKSEYSISIFATDQNHSLLREKRLLRYLQR", "aa_hexdigest": "047f2384cd575639ee22aeebe8a3d49d", "start_type": "ATG", "rbs_motif": "TTATT", "psc": { "uniref90_id": "UniRef90_A0A328EG59", "query_cov": 1.0, "subject_cov": 0.9626865671641791, "identity": 1.0, "score": 489.0, "evalue": 2.64e-174, "valid": true, "uniref50_id": "UniRef50_A0A328EG59" }, "pscc": { "uniref50_id": "UniRef50_A0A328EG59", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EG59" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 30304.910900000046, "isoelectric_point": 9.462685585021976 }, "id": "NKOCPLAKPK_587", "locus": "GCA003260355_00551" }, { "type": "cds", "sequence": "contig_1", "start": 293373, "stop": 293660, "strand": "-", "frame": 2, "gene": null, "product": "DUF5667 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EFS8", "UniRef:UniRef90_A0A328EFS8" ], "nt": "ATGATCCAGGGAGCAATGGAAGATTTTGAGAACGAGTTACTAAGCAAGAGTAAACTCTTCCTCCAAAATACCCTCCTTAAGAAACAGCAAGGGCTCCAGACCGCAATCATCGAAACTGAAAAAGAGCTTGCACAAGCCTCAGGAAATACCTTTAATCTCGAGAAAACACTTTCCGAGCTCACCCTAAAATTAGAAATGATTAATCTTCTACTCACAGCAGACTCAATGGATACCTTTATGAAAAACTTTGAAAGCTACTTAACTTTATTTCCTTTGCCAGAGGTATGA", "aa": "MIQGAMEDFENELLSKSKLFLQNTLLKKQQGLQTAIIETEKELAQASGNTFNLEKTLSELTLKLEMINLLLTADSMDTFMKNFESYLTLFPLPEV", "aa_hexdigest": "e1501492be252f564d8f18f87949052b", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 293367, "stop": 293954, "observations": { "mutation": [ 293375, 293738 ], "stop": [ 293375, 293738 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EFS8", "query_cov": 1.0, "subject_cov": 0.46798029556650245, "identity": 1.0, "score": 177.0, "evalue": 1.17e-54, "gene_start": 1, "gene_end": 95, "reference_start": 107, "reference_end": 201, "reference_sequence": "MKKLIFLGVLLSSLLRGTASTYAASNFQKKLSCKVTENAVRVYLVQESETLKCQEYLTVINSYLKTAYQDLTQIMNNLNRGDDRSYRSSLYESKKKLFLKLASQKNMIQGAMEDFENELLSKSKLFLQNTLLKKQQGLQTAIIETEKELAQASGNTFNLEKTLSELTLKLEMINLLLTADSMDTFMKNFESYLTLFPLPEVGK" }, "paralog": false, "identity": 0.9897435897435898, "score": 366.0, "evalue": 9.88e-132, "description": "Internal stop codon at 293375, 293738. Nonsense mutation around 293375, 293738.", "psc": { "uniref90_id": "UniRef90_A0A328EFS8", "product": "DUF5667 domain-containing protein", "uniref50_id": "UniRef50_A0A328EFS8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EFS8", "UniRef:UniRef50_A0A328EFS8" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EFS8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EFS8" ], "product": "DUF5667 domain-containing protein" } }, "id": "NKOCPLAKPK_588", "locus": "GCA003260355_00552" }, { "type": "cds", "sequence": "contig_1", "start": 294079, "stop": 294408, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF21694.2" ], "nt": "ATGGTAGAGGGAGATTCTTTTGCATTTTTTGATCAGCTTTTTTCTGATCCGGTGCTCGCAGCCCAGACGCTTGAAAAAATGAAGAGCGACGGAGAAAACCGAAACACCGCGAACGGACTTTTGACCCGAGGACTGAAAGTATATCTTACGCTGCTAGATTATCATCAACAAGGCATCACTTCAGCCAAAGAAATCATCGCACAGACCAAGCTTAACCCTTTTGTCGTCCACAAAAACCTAAAAAACATTAGCATATTGCTTAGCTACCAAGACTTTATCAAAAGCTTCTTTAAACAGCTCATCGAACTCGAATTTGCTATCAAAACTTGA", "aa": "MVEGDSFAFFDQLFSDPVLAAQTLEKMKSDGENRNTANGLLTRGLKVYLTLLDYHQQGITSAKEIIAQTKLNPFVVHKNLKNISILLSYQDFIKSFFKQLIELEFAIKT", "aa_hexdigest": "779bac6092509fa97419859cbc46fd3f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF21694.2", "name": "DNA_pol3_delta_C", "length": 104, "aa_cov": 0.944954128440367, "hmm_cov": 0.9711538461538461, "evalue": 5.423647951444504e-08, "score": 29.338605880737305, "start": 7, "stop": 109 } ], "seq_stats": { "molecular_weight": 12402.172899999987, "isoelectric_point": 6.721972465515138 }, "id": "NKOCPLAKPK_589", "locus": "GCA003260355_00553" }, { "type": "cds", "sequence": "contig_1", "start": 294466, "stop": 295008, "strand": "-", "frame": 1, "gene": "holA", "product": "DNA polymerase III subunit delta", "db_xrefs": [ "EC:2.7.7.7", "GO:0003677", "GO:0003887", "GO:0006260", "SO:0001217", "UniRef:UniRef50_A0A7W4EB66", "UniRef:UniRef90_A0A328EI31" ], "nt": "ATGTATGCTTCTGTTTTCCTTTTCTATGGCGAAAATACTTACGCATTAAGACAAGAACTCCAAAGGCGAAAAGTAAACTTCTTGGAAAGATACGGGCAAGATGCACTTTTTCACTTTTCCAATCAGAACCGAGACCTTGGGATGATCAAGCAGGCACTCTATGCTGGTGGACTTTTCGTTTCCAAGAAAATGGTAATCATTGAGGGAGTACCAAAAGACACTTCAGAAGGGAATACCCTTTCAGCAGATAAAATTGAGAAATTTTTTGAGGATTTCCAGCAAAATATCGACAAACTCCCAGCAGATACGATCGTGATTCTCCTTTCTAATAAACCCGACAAAAGAACCAAACCTTTCAAACGATGCATGGACAATCTCCAGCTCAAAAACTTTGAACCTTACAAAGAACCACAACTAAAAACGCTCATTACCAGTCAGCTCGAGCCACTCAAGATCGGGAACGAAGAATTAAGCTACTTCCTCCTAAAAATCGGCGACGATCCTTACCGTTTAGTAAGCGAAGCAGAAAAATTAAAATACTGA", "aa": "MYASVFLFYGENTYALRQELQRRKVNFLERYGQDALFHFSNQNRDLGMIKQALYAGGLFVSKKMVIIEGVPKDTSEGNTLSADKIEKFFEDFQQNIDKLPADTIVILLSNKPDKRTKPFKRCMDNLQLKNFEPYKEPQLKTLITSQLEPLKIGNEELSYFLLKIGDDPYRLVSEAEKLKY", "aa_hexdigest": "47d16f09a92629492ded22891b760b72", "start_type": "ATG", "rbs_motif": "TTCTT", "genes": [ "holA" ], "truncated": "5-prime", "pseudogene": { "start": 294163, "stop": 295008, "observations": { "mutation": [ 294468 ], "stop": [ 294468 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EI31", "query_cov": 1.0, "subject_cov": 0.5438066465256798, "identity": 1.0, "score": 357.0, "evalue": 1.78e-122, "gene_start": 1, "gene_end": 180, "reference_start": 1, "reference_end": 180, "reference_sequence": "MYASVFLFYGENTYALRQELQRRKVNFLERYGQDALFHFSNQNRDLGMIKQALYAGGLFVSKKMVIIEGVPKDTSEGNTLSADKIEKFFEDFQQNIDKLPADTIVILLSNKPDKRTKPFKRCMDNLQLKNFEPYKEPQLKTLITSQLEPLKIGNEELSYFLLKIGDDPYRLVSEAEKLKYGLPPATSQITKEQIDEYCFGMVEGDSFAFFDQLFSDPVLAAQTLEKMKSDGENRNTANGLLTRGLKVYLTLLDYHQQGVTSAKEIIAQTKLNPFVVHKNLKNISILLSYQDFIKNFFKQLIELEFAIKTGKYPESYYRLATKSILLSQHQK" }, "paralog": false, "identity": 0.9928825622775801, "score": 556.0, "evalue": 1.7e-203, "description": "Internal stop codon at 294468. Nonsense mutation around 294468.", "psc": { "uniref90_id": "UniRef90_A0A328EI31", "gene": "holA", "product": "DNA polymerase III subunit delta", "ec_ids": [ "2.7.7.7" ], "uniref50_id": "UniRef50_A0A7W4EB66", "go_ids": [ "GO:0003677", "GO:0003887", "GO:0006260" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EI31", "EC:2.7.7.7", "UniRef:UniRef50_A0A7W4EB66", "GO:0003677", "GO:0003887", "GO:0006260" ] }, "pscc": { "uniref50_id": "UniRef50_A0A7W4EB66", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7W4EB66" ], "product": "DNA polymerase III delta subunit-like C-terminal domain-containing protein" } }, "id": "NKOCPLAKPK_590", "locus": "GCA003260355_00554" }, { "type": "cds", "sequence": "contig_1", "start": 295243, "stop": 295707, "strand": "-", "frame": 1, "gene": null, "product": "DUF4825 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MS74", "UniRef:UniRef90_A0A660MS74" ], "nt": "ATGAAAAACATACAGCGAGCTTTGCTCCTTCTCGGCGGAATCCTACTCATCGGAATCACGACCTTGCTGCGACCCAAAGCTTCTAAAGCTCCTCAAGCTGCCGAGCAGCGACTTACCAAATACGAGCAAACCGCACTTACCGACACCGCCACCATCAAAAAGCTCTTAAAACAGTTCAGTGAGTATGCTGATTTTTCGATTAGCACCGAACAGAGACCCTACACCTTTGCCATCAACCATTTCAATGCCAGTGAGGGAAAAAATATCGGTTTTGACTTCTCAAGCGACAGAGAAAGGAACCAGACGCTCCTCTATCGTGCCTCGCTCCTCTTTCTGCTGATTCCCTCCGTCGAAAAAATCACGATAACCTACGGAGAGGATTCAGTCTTTTCTGCTCTTAAAAAAGACCTCGAAACGCTACTCCAGCTGAGATTTGAAGACTATAGCAAGGACAGTAACGCTTGA", "aa": "MKNIQRALLLLGGILLIGITTLLRPKASKAPQAAEQRLTKYEQTALTDTATIKKLLKQFSEYADFSISTEQRPYTFAINHFNASEGKNIGFDFSSDRERNQTLLYRASLLFLLIPSVEKITITYGEDSVFSALKKDLETLLQLRFEDYSKDSNA", "aa_hexdigest": "2f0a27b975dacbf8d8811571f6eb620f", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A660MS74", "query_cov": 1.0, "subject_cov": 0.806282722513089, "identity": 0.968, "score": 282.0, "evalue": 2.48e-95, "valid": true, "product": "DUF4825 domain-containing protein", "uniref50_id": "UniRef50_A0A660MS74" }, "pscc": { "uniref50_id": "UniRef50_A0A660MS74", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MS74" ], "product": "DUF4825 domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_591", "locus": "GCA003260355_00555" }, { "type": "cds", "sequence": "contig_1", "start": 296216, "stop": 296482, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAGGAAGGATTCAGATTTTTTGAGTTTTTTCATGGTTTGGGAGGAGGGGGAGGTTGGAAAGTTTGGATTTTCTCTGAGTTAAAGCGGGTTTTGACAGAAGCAGAGGTTGATTTTGGAGTTATAGGGGCTTGGGAGCCGTGTGATCTGGGCGAAATGCGTGCTGAGGAGGGATTGCAGATGAGGGAATTGTGCTGCTTCGGATTTGGTCAGGAGATGGACTTCACAGAGGAGGGTTTTGATGCAAGATCGGGTCGCGGGCTCTAG", "aa": "MEEGFRFFEFFHGLGGGGGWKVWIFSELKRVLTEAEVDFGVIGAWEPCDLGEMRAEEGLQMRELCCFGFGQEMDFTEEGFDARSGRGL", "aa_hexdigest": "4eccc1f9a1f40391619966dc97b6ffa4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9970.123999999996, "isoelectric_point": 4.374124336242676 }, "id": "NKOCPLAKPK_592", "locus": "GCA003260355_00556" }, { "type": "cds", "sequence": "contig_1", "start": 296540, "stop": 296965, "strand": "-", "frame": 3, "gene": "fkbM", "product": "Methyltransferase FkbM domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EJM1", "UniRef:UniRef90_A0A328EJM1" ], "nt": "ATGCTAGCTTCCTCTGCCCAGCTCGGGAAATACGAGCAAGCCCTCTACTACGAAATCTTCATCAAGGAGGAGTACCGCATCGCCAAGCCTACCATCAAAAACGCAAAAACCATCTTTGATATCGGCGGCCACCTCGGATTTTTCTCTGAACGATGCCTTTCACTCAATCCACACCTCCAGCTCCATTATTTCGAACCCTTTGACGCCCTCTTCCAGCAGGCAAAAATCAGACTTCAAGACTTTGCACCTCAAATCACCTTCAATCCCTTCGCTATCGCTAGCTCCACAGGCACAGCAACCTTCACCTTCAATGCATCCAAGACCATGCAGTCGAGTAAAGCGGCATCTTTTCTCAATCCCGCAGGTATTCCTCAACAGATCCATACGCAATCACTCAATCAGTATATCAAAGAGCAAAAAATCTGA", "aa": "MLASSAQLGKYEQALYYEIFIKEEYRIAKPTIKNAKTIFDIGGHLGFFSERCLSLNPHLQLHYFEPFDALFQQAKIRLQDFAPQITFNPFAIASSTGTATFTFNASKTMQSSKAASFLNPAGIPQQIHTQSLNQYIKEQKI", "aa_hexdigest": "64039699fd8c1254c17029ea57d2ce6f", "start_type": "ATG", "rbs_motif": "TTTA", "genes": [ "fkbM" ], "truncated": "5-prime", "pseudogene": { "start": 296297, "stop": 296965, "observations": { "mutation": [ 296542 ], "stop": [ 296542 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EJM1", "query_cov": 1.0, "subject_cov": 0.6351351351351351, "identity": 1.0, "score": 276.0, "evalue": 8.08e-93, "gene_start": 1, "gene_end": 141, "reference_start": 1, "reference_end": 141, "reference_sequence": "MLASSAQLGKYEQALYYEIFIKEEYRIAKPTIKNAKTIFDIGGHLGFFSERCLSLNPHLQLHYFEPFDALFQQAKIRLQDFAPQITFNPFAIASSTGTATFTFNASKTMQSSKAASFLNPAGIPQQIHTQSLNQYIKEQKIGSIDLVKLDIEGMEFEVLFALEPATRSCIKTLLCEVHLLTKSEAAQFPHLQSLLSTHFAQITRLPSPYNSKINLCFCQNPL" }, "paralog": false, "identity": 0.9954954954954955, "score": 441.0, "evalue": 3.32e-160, "description": "Internal stop codon at 296542. Nonsense mutation around 296542.", "psc": { "uniref90_id": "UniRef90_A0A328EJM1", "product": "Methyltransferase FkbM domain-containing protein", "uniref50_id": "UniRef50_A0A328EJM1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EJM1", "UniRef:UniRef50_A0A328EJM1" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EJM1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EJM1" ], "product": "Methyltransferase FkbM domain-containing protein" } }, "id": "NKOCPLAKPK_593", "locus": "GCA003260355_00557" }, { "type": "cds", "sequence": "contig_1", "start": 297025, "stop": 297420, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAAATATCGAAAAATCTGGATAAAAAATCCCCTTCATCTGCTCTTCATAAGTTATTTCTGTGTTAGCACGGGAGTCTCTGTGTTCTACTTTGTCAAAGACCGAATCCTCATCAATACCATTATAGAAGCAAATCAATCTTGGACCGCTATCTTCTTCATCTTAAATCCTAAACGGCGACTAACCACAATCTTTACCAAGCTAGGCTTCACCCTCTTTGCACTCCCTTATACCATTCCGCTCATCAAATCTCGAAAAGACCTCCGAAAAATCATTTACATCATCCCTTTTTCTCTCGTCTATATTCCACTATTCTTCGTAGTAAACATCGGCGGCATCATCTCTTGTAGCACCAAATACCGTGCCCTTGAATCAGGGGAAAGAAGAGGCCGATAA", "aa": "MKYRKIWIKNPLHLLFISYFCVSTGVSVFYFVKDRILINTIIEANQSWTAIFFILNPKRRLTTIFTKLGFTLFALPYTIPLIKSRKDLRKIIYIIPFSLVYIPLFFVVNIGGIISCSTKYRALESGERRGR", "aa_hexdigest": "2edb0aedb1bf7ff2323801ff292972a8", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15377.309999999974, "isoelectric_point": 10.36292209625244 }, "id": "NKOCPLAKPK_594", "locus": "GCA003260355_00558" }, { "type": "cds", "sequence": "contig_1", "start": 297529, "stop": 297711, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAACACATCGAATATGTAATGTTAGCCTATATACAAGGTTCTTATAATATCATTAGTGCAATGGGAATGCGAGGAGGCTGTATTATGGTAGACAAAAAAGCTTTTCTAGAGGTCGGTTCTTTTTCCTATCAAGCTATTATTGAAGATATGGATTTAGCATTCAAACTTAATAAAGCCTGA", "aa": "MQHIEYVMLAYIQGSYNIISAMGMRGGCIMVDKKAFLEVGSFSYQAIIEDMDLAFKLNKA", "aa_hexdigest": "3ec3f2b09a9086be2398893535fb41be", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6755.9193, "isoelectric_point": 5.563707542419433 }, "id": "NKOCPLAKPK_595", "locus": "GCA003260355_00559" }, { "type": "cds", "sequence": "contig_1", "start": 297769, "stop": 298128, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00535.33", "PFAM:PF13641.12" ], "nt": "TTGGATCACCAGAAAAAAAGAAAAAAAATTGAAGGCACTCCGCTTTCAGCCTTCATCCCCTGCTACAACGACGGAGCAACAATTGAAACAACCCTCAAAAGCCTCTATGCAAGCTATCCCCACGAGCTGTTAGAGGTTTTCGTGATAAACGACAAAAGCACCGACAATTCGCTAGCAATCCTCGAGAAGCTCAACCAAAGCTACCCCTTCCACTTGATCAACAACAAGGAAAACCTCGGAAAGTCCAAATCCTTAAACGAAATAATCCCCGCTGCAAGCCATGCTACCCTACTCATTATTGATGCTGATACCAAAATCTCCAAAGCAAATATCGCTGATATGCTCAAGAGAAAAGTCTGA", "aa": "MDHQKKRKKIEGTPLSAFIPCYNDGATIETTLKSLYASYPHELLEVFVINDKSTDNSLAILEKLNQSYPFHLINNKENLGKSKSLNEIIPAASHATLLIIDADTKISKANIADMLKRKV", "aa_hexdigest": "2bc4209f50d5cf14ad7993b7a8705524", "start_type": "TTG", "rbs_motif": "TTATT", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00535.33", "name": "Glycos_transf_2", "length": 102, "aa_cov": 0.8487394957983193, "hmm_cov": 0.9901960784313726, "evalue": 6.1076258684891804e-24, "score": 81.28215789794922, "start": 16, "stop": 116 }, { "id": "PF13641.12", "name": "Glyco_tranf_2_3", "length": 101, "aa_cov": 0.7899159663865546, "hmm_cov": 0.9801980198019802, "evalue": 9.590822201392778e-13, "score": 45.0977897644043, "start": 15, "stop": 108 } ], "seq_stats": { "molecular_weight": 13311.196599999974, "isoelectric_point": 8.62827129364014 }, "id": "NKOCPLAKPK_596", "locus": "GCA003260355_00560" }, { "type": "cds", "sequence": "contig_1", "start": 298209, "stop": 298460, "strand": "-", "frame": 2, "gene": null, "product": "ABC transporter ATP-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A948F714" ], "nt": "ATGGATGAGGCAACATCAGCACTTGATAGCGAATCAGAATTCCTTATCCAAGAAGCCATAGAAGAGCTGATGCAAAACAAAACCCTCTTAATTATCGCCCACAGACTCTCAACCATTATGAAAATGGACAAAATCATCGTGATGGATCAAGGGAAAATCGCAGAGAAAGGCTCACACAAAGAGCTCCTTGCAAAAGACAACGGAATCTATAAAAAATTACGGAATATCCAATCTGGTGGATTTATTGGATAG", "aa": "MDEATSALDSESEFLIQEAIEELMQNKTLLIIAHRLSTIMKMDKIIVMDQGKIAEKGSHKELLAKDNGIYKKLRNIQSGGFIG", "aa_hexdigest": "c3f9c52bcd14a6d94fbb46d861b15e22", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A948F714", "query_cov": 1.0, "subject_cov": 0.8556701030927835, "identity": 0.627, "score": 103.0, "evalue": 3.32e-27, "valid": false, "product": "ABC transporter ATP-binding protein", "uniref50_id": "UniRef50_A0A948F714" }, "pscc": { "uniref50_id": "UniRef50_A0A948F714", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A948F714" ], "product": "ABC transporter ATP-binding protein" }, "genes": [], "id": "NKOCPLAKPK_597", "locus": "GCA003260355_00561" }, { "type": "cds", "sequence": "contig_1", "start": 298521, "stop": 298712, "strand": "-", "frame": 2, "gene": null, "product": "Putative ABC transporter ATP-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1V5ZQX2" ], "nt": "ATGTCTATGATTCCTCAAGATCCAATTCTCTTTCATCGTTCCATCAGAGAAAATATCGCCTATGGAAAACCTAACGCAAGTGATGAAGAAATCATTGCTGCTGCCAAAATGGCGAGATGCCACGAATTTATTTCTCATCTCAAGGACAGTTATGAAACTTTAGTTGGAGAAAGGGGAATCAAACTTTCATGA", "aa": "MSMIPQDPILFHRSIRENIAYGKPNASDEEIIAAAKMARCHEFISHLKDSYETLVGERGIKLS", "aa_hexdigest": "7e5f2e25295276bc63a91e1121ae868b", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A1V5ZQX2", "query_cov": 0.8412698412698413, "subject_cov": 1.0, "identity": 0.736, "score": 87.0, "evalue": 1.62e-21, "valid": false, "product": "Putative ABC transporter ATP-binding protein", "uniref50_id": "UniRef50_A0A1V5ZQX2" }, "pscc": { "uniref50_id": "UniRef50_A0A1V5ZQX2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1V5ZQX2" ], "product": "Putative ABC transporter ATP-binding protein" }, "genes": [], "id": "NKOCPLAKPK_598", "locus": "GCA003260355_00562" }, { "type": "cds", "sequence": "contig_1", "start": 299016, "stop": 299165, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATATTGCTCTTCGTATGCGAGGTGCAGGAGTAATCTCAATCGGTATGATCGTTTTGTTGCAAACTTATGTATTGAAAATTATGGACTTCCTTCAAGGGATTGGTCAGACATTAAGAAGCCTCTTCAGATCGATAACAGAAATCTGA", "aa": "MYIALRMRGAGVISIGMIVLLQTYVLKIMDFLQGIGQTLRSLFRSITEI", "aa_hexdigest": "de7c4964b01219cc24c56b4252f22d79", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5531.710299999998, "isoelectric_point": 10.255969047546387 }, "id": "NKOCPLAKPK_599", "locus": "GCA003260355_00563" }, { "type": "cds", "sequence": "contig_1", "start": 299598, "stop": 300002, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCTTTCTCTGCAAAATATACAGATAAAAGCTATTTCAAAAAGAATTATGGGAATACGATAAAATATCTGCTCTATACATACAAACAGTTTTGGGGATATGGAACAGTAGCACTTATTACGAGGATTATAGGAACAGGATTAATGATCGTTCCTCCTCTTTTTTACAAAGAAATTTTTGATAGCATTACCCAAGTAGGAGGAATTCAAAATGAGATTATGGCACATGCCATCAGCGTACTCTTTGTGCTTTTTGGGATCCAAATAGGAACTTTTACCGTATATAGAATCTATGAATATTTTTTCGTCTCCTTTGAGATGAAAAACCAAGAATGGTTAAATAACACCTTTCTTCAGAAACTCCAACAACATTCTTTCCAGTTTTTTACTGATAATTTTACTTGA", "aa": "MSFSAKYTDKSYFKKNYGNTIKYLLYTYKQFWGYGTVALITRIIGTGLMIVPPLFYKEIFDSITQVGGIQNEIMAHAISVLFVLFGIQIGTFTVYRIYEYFFVSFEMKNQEWLNNTFLQKLQQHSFQFFTDNFT", "aa_hexdigest": "0d1703d1d9bdce72fa1fdb65dbe4d46d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15859.15239999999, "isoelectric_point": 9.047831535339355 }, "id": "NKOCPLAKPK_600", "locus": "GCA003260355_00564" }, { "type": "cds", "sequence": "contig_1", "start": 300087, "stop": 300560, "strand": "-", "frame": 2, "gene": null, "product": "Inorganic pyrophosphatase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A356J7D2", "UniRef:UniRef90_A0A328EGD5" ], "nt": "ATGCACTTAATTAATGACGTCCCAACATTTGTTGACCAACAAAAAGGTTATATCAATGTCGTAGTTGATGTTCCTAGAGGATCAAACAATAAATACGAATACGATCATGTCTTAGGATGTTTCAGACTTGACCGTGTTATTCATAGTAGTATGTACTATCCTGTAGATTACGGCTTCATCCCTCAAACCTTATCTGAAGACGGCGATCCTTGTGATGTATGTCTTCTTACAACCAATCCAACTTTCACCGGTTGCATCATCAAGGCAAGACCTATCGGAATCCTCTATACTTGCGATAATGCAGGAGATGACCCTAAAATTATTGCCGTTCCAACAAGTGACGTAGACCCAAGACGGGACGAAATCCACCATATCGATGATCTCGGACACCATATGAAGGAAGAACTCTTCACGATTTTCAAACAAATCAAATTCCTCGAACACGAAAAATATGACAAAGTAGAAGTGAAATGA", "aa": "MHLINDVPTFVDQQKGYINVVVDVPRGSNNKYEYDHVLGCFRLDRVIHSSMYYPVDYGFIPQTLSEDGDPCDVCLLTTNPTFTGCIIKARPIGILYTCDNAGDDPKIIAVPTSDVDPRRDEIHHIDDLGHHMKEELFTIFKQIKFLEHEKYDKVEVK", "aa_hexdigest": "e148b2ede1bb7bcdc1de0c78e251132f", "start_type": "ATG", "rbs_motif": "TTCTT", "psc": { "uniref90_id": "UniRef90_A0A328EGD5", "query_cov": 1.0, "subject_cov": 0.8626373626373627, "identity": 1.0, "score": 333.0, "evalue": 1.55e-115, "valid": true, "product": "Inorganic pyrophosphatase", "uniref50_id": "UniRef50_A0A356J7D2" }, "pscc": { "uniref50_id": "UniRef50_A0A356J7D2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A356J7D2" ], "product": "Inorganic pyrophosphatase" }, "genes": [], "id": "NKOCPLAKPK_601", "locus": "GCA003260355_00565" }, { "type": "cds", "sequence": "contig_1", "start": 300671, "stop": 301222, "strand": "+", "frame": 2, "gene": "ruvA", "product": "Holliday junction branch migration protein RuvA", "db_xrefs": [ "EC:3.6.4.12", "GO:0003678", "GO:0006281", "GO:0006310", "GO:0009378", "GO:0009379", "SO:0001217", "UniParc:UPI000DC0115A", "UniRef:UniRef100_A0A328EJP0", "UniRef:UniRef50_A0A2W4IKK0", "UniRef:UniRef90_A0A328EJP0" ], "nt": "ATGCTACATTATTTTCATGGAGAAATAAAAAGGGTGAAGTCTGAAACCTTCCTCGTGAATCCTTATTTCGGGGTTCAGGTTGCTTATGCTGGTGGGCAGTCTCAGGGAGAATTTTTCCTTTATCCTTATTTGGATGATCAAAAAAAGTCAGTTTTCTATTTTGCTTTTGATACTTTTGATCAGAAGGAATGGTTTGAAAGAATGTTGAAAATCTCTGGAATTGGACCGAAAATTGCTTTTCAGATAGTGAAGATTCCGATGCAAGAATTGGCAGCTGCGATTGACCGCTTGGATCCCAAAATTTTTCAGACGATTTCTGGAGTTGGTCCGAAGCTTGCGAAGAAAATTATTCTTGAACTGAAGGGAAATTTTGATTTTTCAGAAGTTGCTGCTGTCAACCAGGATCAAAAGCTTTTTAAGGCGGTAGTAAAGTCGCTTCAAAATCTGGGATACGATGTCCAAGAAGTGAAATCAATTCTAGCTAGCTATCCGAAACCTATCGAAAAGGAGCATCTTGCTGAGGTGGTTAAGTGGGTGATTGCTAAGATGTAA", "aa": "MLHYFHGEIKRVKSETFLVNPYFGVQVAYAGGQSQGEFFLYPYLDDQKKSVFYFAFDTFDQKEWFERMLKISGIGPKIAFQIVKIPMQELAAAIDRLDPKIFQTISGVGPKLAKKIILELKGNFDFSEVAAVNQDQKLFKAVVKSLQNLGYDVQEVKSILASYPKPIEKEHLAEVVKWVIAKM", "aa_hexdigest": "856e4824f74c6a5a9b6ff742bc3b2429", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000DC0115A", "uniref100_id": "UniRef100_A0A328EJP0", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DC0115A", "UniRef:UniRef100_A0A328EJP0" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EJP0", "uniref90_id": "UniRef90_A0A328EJP0" }, "psc": { "uniref90_id": "UniRef90_A0A328EJP0", "gene": "ruvA", "product": "Holliday junction branch migration protein RuvA", "ec_ids": [ "3.6.4.12" ], "uniref50_id": "UniRef50_A0A2W4IKK0", "go_ids": [ "GO:0003678", "GO:0006281", "GO:0006310", "GO:0009378", "GO:0009379" ] }, "pscc": { "uniref50_id": "UniRef50_A0A2W4IKK0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4IKK0" ], "product": "Holliday junction branch migration complex subunit RuvA" }, "genes": [ "ruvA" ], "id": "NKOCPLAKPK_602", "locus": "GCA003260355_00566" }, { "type": "cds", "sequence": "contig_1", "start": 301276, "stop": 301734, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00464.25" ], "nt": "ATGAATACTATCGCAGGAATCACTGTCGCACTAGGTGAAGTTCAGACTCCAGCTTTTCAGCATTACGCCAAACAAACCCTCAAAAACGCCCAAATGATGGCACAAACCTTCCTCAGTTTAGGCTACAAACTCGTAACAGGAGGGACAGACAATCATATGGTTATCCTAGACTTTTCACAAGAGCCTTTTAACGGAGCTGAAATTGAAAACATACTTGACCAAATTGGAATCTCAGTTTCCAAATCACTCATTCCTCACGATCCACGTCCTCCATTCCGCCCCTCTGGAATAAGGATCGGGATAGCCGCAATGACGACAAGAGGAATTACCGAAAACGAAGTCAAAAAGATCGTCTCCTTCATTGATAGAGCAATCCACAACCGCGAAAATCCAGCAACTTTAGATGCATTAAGGCAAGAAGTTATTACTTTTTCAAAAAATTTTCCACTCCCGCACTAA", "aa": "MNTIAGITVALGEVQTPAFQHYAKQTLKNAQMMAQTFLSLGYKLVTGGTDNHMVILDFSQEPFNGAEIENILDQIGISVSKSLIPHDPRPPFRPSGIRIGIAAMTTRGITENEVKKIVSFIDRAIHNRENPATLDALRQEVITFSKNFPLPH", "aa_hexdigest": "cb56ba99fe091c36c12e400c4f52f858", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00464.25", "name": "SHMT", "length": 121, "aa_cov": 0.7960526315789473, "hmm_cov": 0.9917355371900827, "evalue": 2.747836661238265e-38, "score": 128.01686096191406, "start": 1, "stop": 121 } ], "seq_stats": { "molecular_weight": 16782.096199999985, "isoelectric_point": 6.9728597640991214 }, "id": "NKOCPLAKPK_603", "locus": "GCA003260355_00567" }, { "type": "cds", "sequence": "contig_1", "start": 301984, "stop": 302169, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00464.25" ], "nt": "GTGACCTTTATTTCCAAAGTTTTCAAATTTCACAGCTATGGAACTTTGCCTGATGGGACAATTGATTTTGCCCAAGTTCGTAGCTTAGCTTTGGAGATGAAACCAAAGGTGATTTTAGCGGGATTTAGTGCCTATCCAAGAGAGCTTGATTATGCGAGATTCGCAGAAATAGCAAAAGAAGTCTGA", "aa": "MTFISKVFKFHSYGTLPDGTIDFAQVRSLALEMKPKVILAGFSAYPRELDYARFAEIAKEV", "aa_hexdigest": "8777166d0aaf3d00bb68e848e49e6657", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00464.25", "name": "SHMT", "length": 61, "aa_cov": 0.9836065573770492, "hmm_cov": 1.0, "evalue": 8.703747870181762e-15, "score": 50.63605499267578, "start": 2, "stop": 61 } ], "seq_stats": { "molecular_weight": 6915.9831, "isoelectric_point": 8.056307792663574 }, "id": "NKOCPLAKPK_604", "locus": "GCA003260355_00568" }, { "type": "cds", "sequence": "contig_1", "start": 302383, "stop": 302550, "strand": "-", "frame": 1, "gene": null, "product": "Serine hydroxymethyltransferase 2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1V5ZKS3" ], "nt": "ATGCAAATTTCTATCAAAGATAAAGAAGTTCAAAATCTGATCCAATCCGAAGAACAGAGACAAATAATGGGAATTGAGCTTATTGCAAGTGAAAATTACCAATCTTCTGCAGTTCTTCAGGCTCAGGCTTCAGTTTTTGCCAATAAATATGCTGAAGGTTTCCCTTGA", "aa": "MQISIKDKEVQNLIQSEEQRQIMGIELIASENYQSSAVLQAQASVFANKYAEGFP", "aa_hexdigest": "506a3a9e5d348ad1406021113995850b", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A1V5ZKS3", "query_cov": 0.9454545454545454, "subject_cov": 1.0, "identity": 0.654, "score": 63.9, "evalue": 1.62e-12, "valid": false, "product": "Serine hydroxymethyltransferase 2", "uniref50_id": "UniRef50_A0A1V5ZKS3" }, "pscc": { "uniref50_id": "UniRef50_A0A1V5ZKS3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1V5ZKS3" ], "product": "Serine hydroxymethyltransferase 2" }, "genes": [], "id": "NKOCPLAKPK_605", "locus": "GCA003260355_00569" }, { "type": "cds", "sequence": "contig_1", "start": 302949, "stop": 303305, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EFT8", "UniRef:UniRef90_A0A328EFT8" ], "nt": "ATGAATATATTCTTACAATGCGATTTTGATCATCCACTAATTCAAAACTTTTATCAAAAAGTAGACGAAGGCTATAATACTGCTATTGAAGATCCAAAATTCAAAGAATGGTATGAAACGAAGAAGCTACCTATTCGTGGAGAACTCTTTTTGCGTCTTTCTGCCTATACTTCAATATTTAATAAAATGTTCACGAGAAAAGAAAACTCTGCTCGAGAAAGGCATGAGTATTATAATAAGCAGGAAGTTGCATTGCTATCTGAAATCCTAGAGAAAGGGATATTTATGTGTGCAGAACTAGCTTCGTTAGCACATTACTATCTTAAGACTCATGGGGTTGACAATTTTTATGTTTGA", "aa": "MNIFLQCDFDHPLIQNFYQKVDEGYNTAIEDPKFKEWYETKKLPIRGELFLRLSAYTSIFNKMFTRKENSARERHEYYNKQEVALLSEILEKGIFMCAELASLAHYYLKTHGVDNFYV", "aa_hexdigest": "0898d02d90d3fc6b6358ee3a9bb1f11d", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 302949, "stop": 303425, "observations": { "mutation": [ 303303, 303306 ], "stop": [ 303303, 303306 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EFT8", "query_cov": 1.0, "subject_cov": 0.7468354430379747, "identity": 1.0, "score": 246.0, "evalue": 2.76e-82, "gene_start": 1, "gene_end": 118, "reference_start": 1, "reference_end": 118, "reference_sequence": "MNIFLQCDFDHPLIQNFYQKVDEGYNTAIEDPKFKEWYETKKLPIRGELFLRLSAYTSIFNKMFTRKENSARERHEYYNKQEVALLSEILEKGIFMCAELASLAHYYLKTHGVDNFYVGGELLPKLYEESEELRGGRHIALFTLETIIFNLFMIQHFL" }, "paralog": false, "identity": 0.9873417721518988, "score": 318.0, "evalue": 4.95e-113, "description": "Internal stop codon at 303303, 303306. Nonsense mutation around 303303, 303306.", "psc": { "uniref90_id": "UniRef90_A0A328EFT8", "uniref50_id": "UniRef50_A0A328EFT8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EFT8", "UniRef:UniRef50_A0A328EFT8" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EFT8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EFT8" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 14148.991199999986, "isoelectric_point": 6.074690055847167 }, "id": "NKOCPLAKPK_606", "locus": "GCA003260355_00570" }, { "type": "cds", "sequence": "contig_1", "start": 303640, "stop": 303990, "strand": "-", "frame": 1, "gene": null, "product": "Phosphate propanoyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A4R4EHG3", "UniRef:UniRef90_A0A660MNT0" ], "nt": "ATGCTCGGAGATACGTTCAAACTTGGGACTCCAACTCCGATCAGAGAATCAGGAGATTTAGACGGATCAGCTCCAGTGACGATCATTGGTCCGGCAGGTCAGGTTGAGCTTACTCAAGGAATGATTGTTGCCAAGAGACATATTCATATGACGGTGGCTGATGCAGAGAATTTTGGGGTAACGAACGGACAAATCGTGAAGGTAAAGACCAACGGAGAAAGAAGTCTGATTTTTGATGAGGTAGTGATTAGAGTTTCAGATAGTTATGCTCTTGATATGCATATTGATGTAGAAGAAGGGAATGCTGCTGGGCTTAAGCAAGGTGATTGGGGAGAACTCATTAAAGACTAA", "aa": "MLGDTFKLGTPTPIRESGDLDGSAPVTIIGPAGQVELTQGMIVAKRHIHMTVADAENFGVTNGQIVKVKTNGERSLIFDEVVIRVSDSYALDMHIDVEEGNAAGLKQGDWGELIKD", "aa_hexdigest": "b762e8280c675d1e944e911a7dde5106", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0034D9564E", "query_cov": 0.9655172413793104, "subject_cov": 0.9256198347107438, "identity": 0.518, "score": 120.0, "evalue": 6.99e-33, "valid": false, "product": "PduL/EutD family phosphate acyltransferase", "uniref50_id": "UniRef50_A0A8S5RTP3" }, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 303640, "stop": 304209, "observations": { "mutation": [ 304050 ], "stop": [ 304050 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A660MNT0", "query_cov": 1.0, "subject_cov": 0.6137566137566137, "identity": 0.9740000000000001, "score": 224.0, "evalue": 2.44e-73, "gene_start": 1, "gene_end": 116, "reference_start": 74, "reference_end": 189, "reference_sequence": "MRIPIGISNRHVHLSQADANTLFGPNYELTKLKDLSQPGQFACEECVTIKGAKGEISKVRILGPYRKFSQVEVMLGDTFKLGTPTPIRESGDLEGSAPVTIIGPAGQVELTQGMIVAKRHIHMTVADAENFGVTNGQIVKVKTNGERSLIFDEVVIRVSDSYALDMHIDVEEGNAAGLKQGDRGELIKE" }, "paralog": false, "identity": 0.9788359788359788, "score": 367.0, "evalue": 9.37e-132, "description": "Internal stop codon at 304050. Nonsense mutation around 304050.", "psc": { "uniref90_id": "UniRef90_A0A660MNT0", "product": "Phosphate propanoyltransferase", "uniref50_id": "UniRef50_A0A4R4EHG3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A660MNT0", "UniRef:UniRef50_A0A4R4EHG3" ] }, "pscc": { "uniref50_id": "UniRef50_A0A4R4EHG3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A4R4EHG3" ], "product": "Phosphate propanoyltransferase" } }, "id": "NKOCPLAKPK_607", "locus": "GCA003260355_00571" }, { "type": "cds", "sequence": "contig_1", "start": 304048, "stop": 304209, "strand": "-", "frame": 1, "gene": null, "product": "Phosphate propanoyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4IJC9", "UniRef:UniRef90_A0A2W4IJC9" ], "nt": "ATGAGAATCCCTATCGGAATATCAAACAGGCACGTGCATCTTTCACAGGCTGATGCAAATACCCTTTTCGGACCTAATTATGAGCTCACAAAACTCAAAGACCTCTCTCAACCTGGACAATTTGCATGCGAAGAATGCGTAACGATTAAAGGTGCGAAATGA", "aa": "MRIPIGISNRHVHLSQADANTLFGPNYELTKLKDLSQPGQFACEECVTIKGAK", "aa_hexdigest": "d67f7ab5e4677334461fe6aea02cf88c", "start_type": "ATG", "rbs_motif": "TTTTT", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 304000, "stop": 304209, "observations": { "mutation": [ 304050 ], "stop": [ 304050 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A2W4IJC9", "query_cov": 1.0, "subject_cov": 0.48623853211009177, "identity": 0.981, "score": 111.0, "evalue": 8.5e-31, "gene_start": 1, "gene_end": 53, "reference_start": 1, "reference_end": 53, "reference_sequence": "MRIPIGISNRHVHLSQADANTLFGPNYELTKLKDLSQPGQYACEECVTIKGAKGAIEKVRILGPYRTFSPSYKTKTGPGATARGGSGKRIEISGFSFLKISQATGGFGR" }, "paralog": false, "identity": 0.927536231884058, "score": 132.0, "evalue": 1.59e-41, "description": "Internal stop codon at 304050. Nonsense mutation around 304050.", "psc": { "uniref90_id": "UniRef90_A0A2W4IJC9", "product": "Phosphate propanoyltransferase", "uniref50_id": "UniRef50_A0A2W4IJC9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A2W4IJC9", "UniRef:UniRef50_A0A2W4IJC9" ] }, "pscc": { "uniref50_id": "UniRef50_A0A2W4IJC9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4IJC9" ], "product": "Phosphate propanoyltransferase" } }, "id": "NKOCPLAKPK_608", "locus": "GCA003260355_00572" }, { "type": "cds", "sequence": "contig_1", "start": 304541, "stop": 304717, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGTGGCAAAAAAAGAGATCGAGCAAATTACGATCTTTGGAGGAAACTACATCACGCCAGATGGGACCTGCATTAGAGATTATATTCACGTAGAGGACGTTGCTCAGGCTCATTTACAAGCGTTTGACTATATCCAAAAATACATCCAGCAAAGTAGTGAGGTAGAAAACTGA", "aa": "MKVAKKEIEQITIFGGNYITPDGTCIRDYIHVEDVAQAHLQAFDYIQKYIQQSSEVEN", "aa_hexdigest": "abba1b188b525b915b708a409b2403dc", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6690.437500000004, "isoelectric_point": 4.817638969421386 }, "id": "NKOCPLAKPK_609", "locus": "GCA003260355_00573" }, { "type": "cds", "sequence": "contig_1", "start": 304760, "stop": 305338, "strand": "-", "frame": 3, "gene": null, "product": "UDP-glucose 4-epimerase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B5DC81" ], "nt": "ATGGAGAATAAAAAAATCCTAATTACTGGAGGGCTGGGTTATATTGGAGCACATACCACAGTCCTATTTATACAAGCTGGATATGACGTCGTTATCCTCGATAATCTCTCAAACGCCCACAAAGAATCGGTCCTCGCCGCGATCAAAGAACTCACAGGCAAAGAACCTCTTTTCTACGAAGGAGACATAAGAAACTATGATTATCTTGATAACCTTTTCTCCAAAGAAGAGATTGACGGAGTAATTCACTTTGCTGCGAAAAAAGCAGTGGGAGAGAGTTGCTATGATCCTTTCCTCTATTATGACAACAATATCATTGGAAGCATCAATCTCCTCAAAGTAATGAACAAGCATCAGGTTAAAAAGCTGATTTTCTCCTCTAGTGCAACAGTATACGATACTGAAAAAAATATCCCACCGTTTACAGAAACTGATCGCCTTAGCACCACCAACCCTTATGGAACGACTAAACTCGTCATTGAGAAACTTCTCTACGATATGGCACTCCACAAAGGATTTTCAGCGATTTGCCTCAGATACTTCAATCCCATCGGGGCACATTCTTCAGGTCTTCTCTGA", "aa": "MENKKILITGGLGYIGAHTTVLFIQAGYDVVILDNLSNAHKESVLAAIKELTGKEPLFYEGDIRNYDYLDNLFSKEEIDGVIHFAAKKAVGESCYDPFLYYDNNIIGSINLLKVMNKHQVKKLIFSSSATVYDTEKNIPPFTETDRLSTTNPYGTTKLVIEKLLYDMALHKGFSAICLRYFNPIGAHSSGLL", "aa_hexdigest": "57056863581fce9b1803ff288c13f116", "start_type": "ATG", "rbs_motif": "TTTA", "psc": { "uniref90_id": "UniRef90_A0A2W4IJA1", "query_cov": 1.0, "subject_cov": 0.8421052631578947, "identity": 0.7759999999999999, "score": 321.0, "evalue": 1.2e-109, "valid": false, "product": "UDP-glucose 4-epimerase", "uniref50_id": "UniRef50_B5DC81" }, "pscc": { "uniref50_id": "UniRef50_B5DC81", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B5DC81" ], "product": "UDP-glucose 4-epimerase" }, "genes": [], "id": "NKOCPLAKPK_610", "locus": "GCA003260355_00574" }, { "type": "cds", "sequence": "contig_1", "start": 305520, "stop": 306023, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTTTTTCTTTTATTAATCTTGGTTGCACGAAAAACCTTGTGGATACCCAATTTTTACTTGGAAGGATATTGGAACGTCGACCTGATCAGTTTTTTTATGCTGTTGATCCTTTTTCTGATGAAGTAGAGCTCGTTTTTCTCAATACTTGTGGGTTTATTTCTTCGGGGAGGCAAGAAATGTTTCATACCATGAAAAAGCTCCTGAGAAAAGAGAAAAAAATCTGTCTCTTGGGATGTGCGGTCCAGTATTTTGAGAAATTGGTGAAGGATGCTCGTCTTTCTGATGCAGAAACTCAAGAATGGGAAAGGCTTAAATCGCATCCTGGGCTTACTTTTCTTTCGTGGTCGGAACTTGCGAATTTTGAACCGTCTTTGCTAGAAAAATCGTTGATTGCTGGAGAAAATGAGTTGTTTGATGGGAACGATTTTCAGTGGCAAGAGAATGCAAGAGCTTATACCAATAGTGAACTAGGTTTCGAATATCTCAAGATCGCAGAATGA", "aa": "MFFSFINLGCTKNLVDTQFLLGRILERRPDQFFYAVDPFSDEVELVFLNTCGFISSGRQEMFHTMKKLLRKEKKICLLGCAVQYFEKLVKDARLSDAETQEWERLKSHPGLTFLSWSELANFEPSLLEKSLIAGENELFDGNDFQWQENARAYTNSELGFEYLKIAE", "aa_hexdigest": "3023fef71f46bc8daab34d0d81ac7e95", "start_type": "ATG", "rbs_motif": "TTATT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19461.9717, "isoelectric_point": 4.992305183410643 }, "id": "NKOCPLAKPK_611", "locus": "GCA003260355_00575" }, { "type": "cds", "sequence": "contig_1", "start": 306096, "stop": 306707, "strand": "+", "frame": 3, "gene": null, "product": "MiaB/RimO family radical SAM methylthiotransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MQG9", "UniRef:UniRef90_A0A660MQG9" ], "nt": "GTGATTGCAGAGGCGCGCAATTTGATTGCTTCTGGAGTGAGGGAGTTGATCCTGATCGCCCAAGATAGTACAAGGTATGGAGTCGATCTGTATGGAAAGAGTCAGCTTTTTGAACTTTTGGAAGGAATTGATGCATTGGAGGGAGATTTTCACTATAGGGTGCTGTATCTCTATCCAGATTTGCTTACGAAAAGGCATCTTGAGAGGCTTACCAAACTCAAAAAATTTATCCCGTATTTTGATCTTCCGCTCCAACATGCATCTCCGAAAATTCTCAAGGCAATGGGGAGATTTTACGATCATCAGATGACCCTGGAATTGCTCGATTTTATTGAAGGGCATTTTCCTGAGCGTTTTGTGAGGACGAATTTTATTATTGGGTTTCCGGGTGAGACGGAGGAGGATTTTCAAGCTTTGATGGATTTTATCAGAGGAGATTGGTTTGAGAATATTGCACTCTTTGAATATCATGACGAACCGCTGGCGGCTTCCAGTCTTTTGCCTGATAAAGTAGAAGCGAAAACGATTAGAAAGAGATTTTTGGAGGCGAAAAAATTGGTGGATGAGCTTCAGGCACAGAGAAGCCAGCATGCAGCTAAAGAAAAAACCTGA", "aa": "MIAEARNLIASGVRELILIAQDSTRYGVDLYGKSQLFELLEGIDALEGDFHYRVLYLYPDLLTKRHLERLTKLKKFIPYFDLPLQHASPKILKAMGRFYDHQMTLELLDFIEGHFPERFVRTNFIIGFPGETEEDFQALMDFIRGDWFENIALFEYHDEPLAASSLLPDKVEAKTIRKRFLEAKKLVDELQAQRSQHAAKEKT", "aa_hexdigest": "0420bfa686c82b4def8f97983b087b4a", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 305796, "stop": 306881, "observations": { "mutation": [ 306021, 306705 ], "stop": [ 306021, 306705 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A660MQG9", "query_cov": 1.0, "subject_cov": 0.4481236203090508, "identity": 0.966, "score": 389.0, "evalue": 9.18e-133, "gene_start": 1, "gene_end": 203, "reference_start": 193, "reference_end": 395, "reference_sequence": "MFFSFINLGCTKNLVDTQFLLGRILERRPDQFFYAVDPFSDEVELVFLNTCGFISSGRQEMFHTMKKLLRKKKKICLLGCAVQYFEKLVKDARLSDAETQEWERLKSHPGLTFLSRSELANFEPSLLEKPLIAGEDQLFDGSDFQWQENARAYTNSELGFEYLKIAEGCNNHCSFCIIPTIRGKQRSLSLEAVIAEARNLIASGVRELILIAQDSTRYGVDLYGKSQLFELLEGIDVLEGDFHYRVLYLYPDLLTKKHLERLTKLKKFIPYFDLPLQHASPKILKAMRRFYDHQMTLELLDFIEGHFPERFVRTNFIIGFPGETDEDFQVLMDFIRGDWFENIALFEYHDEPLAASSLLPDKVEAKTIRKRFLEAKKLVDELQAQRSLHAAKEKTGTISELRSDTQGNWRLMVRPFLHCPEIDEEDEIQVGQVMQCFDGEELTLGSRIAYRLP" }, "paralog": false, "identity": 0.9529085872576177, "score": 693.0, "evalue": 3.36e-255, "description": "Internal stop codon at 306021, 306705. Nonsense mutation around 306021, 306705.", "psc": { "uniref90_id": "UniRef90_A0A660MQG9", "product": "MiaB/RimO family radical SAM methylthiotransferase", "uniref50_id": "UniRef50_A0A660MQG9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A660MQG9", "UniRef:UniRef50_A0A660MQG9" ] }, "pscc": { "uniref50_id": "UniRef50_A0A660MQG9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MQG9" ], "product": "MiaB/RimO family radical SAM methylthiotransferase" } }, "id": "NKOCPLAKPK_612", "locus": "GCA003260355_00576" }, { "type": "cds", "sequence": "contig_1", "start": 306753, "stop": 306881, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTGCGTCCTTTCCTTCATTGTCCGGAAATTGATGAGGAAGATGAAATTCAGATTGAGCAGGTGATGCAATGTTTTGATGGGGAGGAACTTACTCTTGGAAGCAGAATCGCCTATCGTTTGCCTTAG", "aa": "MVRPFLHCPEIDEEDEIQIEQVMQCFDGEELTLGSRIAYRLP", "aa_hexdigest": "1472a56b77db7d600604a386526b7924", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4951.588199999998, "isoelectric_point": 4.180701255798339 }, "id": "NKOCPLAKPK_613", "locus": "GCA003260355_00577" }, { "type": "cds", "sequence": "contig_1", "start": 306878, "stop": 307189, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAATACGGTATTTTAGGATTGCTCCCACGATTATTCTGTTTTATTTGGCTGATTCGAATTGGACTCAGAGCGATCAAACCTTTAGCCCTTGAATGCAAAAAAGGAAAAAAAGCTGACCAAAAGAAAATCAATCAGCTTGTTTGCCTCACAGGATTTGGAATAGGGATGCTTGGCTTGGCACTCGAGGGAATGGTATTGCATAGCTTTGTAGATAGGATGGTCGTATATCCGCTGATGTTGCTCTATGGAATCGTTTACGGACAATCAACCGCACCCACACAGACAAAGCAACCACCCAAACCCCACTAA", "aa": "MEYGILGLLPRLFCFIWLIRIGLRAIKPLALECKKGKKADQKKINQLVCLTGFGIGMLGLALEGMVLHSFVDRMVVYPLMLLYGIVYGQSTAPTQTKQPPKPH", "aa_hexdigest": "2bf58edf2cdf5d38cc2334264e78e04c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11500.915799999992, "isoelectric_point": 9.718367195129392 }, "id": "NKOCPLAKPK_614", "locus": "GCA003260355_00578" }, { "type": "cds", "sequence": "contig_1", "start": 307382, "stop": 308083, "strand": "-", "frame": 3, "gene": null, "product": "O-antigen ligase-related domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MP82", "UniRef:UniRef90_A0A328EJM5" ], "nt": "TTGATAGCGTGTGGGATTAGTTTCCTCCTTGCAGTTGGGATCCAAAAAGTAGGATTAGGGACCTATATCTTGTCATTCAAGTATGATCTGATGGGCTTCTTGATCTTCCTCTTGGGAGTTGGGCTCACACGATACCTCCCTCAGCCTCGCCTCAAAATCACCAAGCGATATACAAAACTTCTCCAGCTGGTAGTCATCGGAGGACTCTTCTGGCGAGCGCTCAGCTATTTCATGCCCAATACACTTGATCACCTCGGCTACAACCGTTTTAATTATGAAGGGACAATAGGCTCTAGACCGCCAGCAGTCTATTATACCAATATCAATCAAGGCTTCGTCAGGAACGAGTTTCTCTTTGAGAGACCCATCTATTTCGGATTCTTTTTGGTTGCATTCCGACCATTGTTCGCCCTGAGCTATCTCAGGACCCGCTCCAAAAAAGAGGCATTACTACGAATGATCGGCTACGGACTTTTGGTGTTCTCGACCTTTTCTAGGGCAGCGATCGGGGTCCGACTCCTAGAAACAGCGATTATCATCTTAGCACTCTACCGAGCCTATGCCAAAAGACTCATCTTCGGCATAGGACTACCGCTCGCTTTAGTAGGTGGAGCACTGTTTTGGCACTATAGGGGAATCTTTGCCAGAGAACACTCAAATACGGGACACGCCGCACTTTTGATCGAAGGGCTCAAAATCTGA", "aa": "MIACGISFLLAVGIQKVGLGTYILSFKYDLMGFLIFLLGVGLTRYLPQPRLKITKRYTKLLQLVVIGGLFWRALSYFMPNTLDHLGYNRFNYEGTIGSRPPAVYYTNINQGFVRNEFLFERPIYFGFFLVAFRPLFALSYLRTRSKKEALLRMIGYGLLVFSTFSRAAIGVRLLETAIIILALYRAYAKRLIFGIGLPLALVGGALFWHYRGIFAREHSNTGHAALLIEGLKI", "aa_hexdigest": "3caca460b261d4ddafb0cb63255ff537", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 307079, "stop": 308356, "observations": { "mutation": [ 307225, 307363, 307384, 308089, 308305 ], "stop": [ 307225, 307363, 307384, 308089, 308305 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EJM5", "query_cov": 1.0, "subject_cov": 0.4735772357723577, "identity": 0.996, "score": 453.0, "evalue": 5.07e-157, "gene_start": 1, "gene_end": 233, "reference_start": 92, "reference_end": 324, "reference_sequence": "MKQRIQALLPRLYKIFFGGLLVQFFLHTLITHQLGREGGIRTLIRLWKEIFILLFAGLIGYWIFFCKGKEERKLTWNHLRASELFRFLIGFLIACGISFLLAVGIQKVGLGTYILSFKYDLMGFLIFLLGVGLTRYLPQPRLKITKRYTKLLQLVVIGGLFWRALSYFMPNTLDHLGYNRFNYEGTIGSRPPAVYYTNINQGFVRNEFLFERPIYFGFFLVAFRPLFALSYLRTRSKKEALLRMIGYGLLVFSTFSRAAIGVRLLETAIIILALYRAYAKRLIFGIGLPLALVGGALFWHYRGIFAREHSNTGHAALLIEGLKIGQESPFFGRGAGYSGPASHQVCFNALQSSDPNSQNPRCERIKEVNARYEIPTFGYNPENQYVQIFMEYGILGLLPRLFCFIWLIRIGLRAIKPLALECKKGKKADQKKINQLVCLTGFGIGMLGLALEGMVLHSFVDRMVVYPLMLLYGIVYGQSTAPTQTKQPPKPH" }, "paralog": false, "identity": 0.9882352941176471, "score": 841.0, "evalue": 1.55e-312, "description": "Internal stop codon at 307225, 307363, 307384, 308089, 308305. Nonsense mutation around 307225, 307363, 307384, 308089, 308305.", "psc": { "uniref90_id": "UniRef90_A0A328EJM5", "product": "O-antigen ligase-related domain-containing protein", "uniref50_id": "UniRef50_A0A660MP82", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EJM5", "UniRef:UniRef50_A0A660MP82" ] }, "pscc": { "uniref50_id": "UniRef50_A0A660MP82", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MP82" ], "product": "O-antigen ligase-related domain-containing protein" } }, "id": "NKOCPLAKPK_615", "locus": "GCA003260355_00579" }, { "type": "cds", "sequence": "contig_1", "start": 308087, "stop": 308293, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCAGTTTTTCCTCCATACTTTGATTACACACCAACTCGGTCGAGAGGGAGGCATCCGAACCCTAATCCGACTCTGGAAAGAAATCTTTATTCTTCTCTTTGCAGGGCTTATTGGCTATTGGATCTTTTTCTGTAAGGGAAAAGAAGAAAGAAAGCTGACTTGGAATCACTTGCGCGCTTCGGAACTCTTCAGATTTTTAATCTGA", "aa": "MQFFLHTLITHQLGREGGIRTLIRLWKEIFILLFAGLIGYWIFFCKGKEERKLTWNHLRASELFRFLI", "aa_hexdigest": "e368a9334a8824f84e0c16b44414d138", "start_type": "GTG", "rbs_motif": "TTCTT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8267.808299999992, "isoelectric_point": 10.167969703674316 }, "id": "NKOCPLAKPK_616", "locus": "GCA003260355_00580" }, { "type": "cds", "sequence": "contig_1", "start": 308589, "stop": 309242, "strand": "-", "frame": 2, "gene": null, "product": "Clp ATPase C-terminal domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4KEF2" ], "nt": "ATGTCAGAATTTATGGAAAAATTCTCCGTCTCTAAATTGATCGGTTCGCCTGCCGGCTACATAGGCTACGACGAAGGAGGGGGACTTACGGAAGCCGTGAGGAGAAAACCCTATTCCGTGATCTTGCTTGACGAGATCGAAAAAGCATCCCCCGACGTGCTGAATATCCTCTTGCAGATCCTCGATGAAGGACAACTCAAAGATAGCAAAGGAAGACGGATCGACTTCAAAAATACCATTATCGTGATGACCTCAAATATCGGCACCGAAGAGTTTAGCAAAAAGAAAACCGCTATCGGATTCGATACGGGAAGTAAAAACGATATCGAGACCAAACAGTTCGACGACATCAAAACCAGAGTCCTCGAGGAGCTCAAAAACTTCCTAACCCCAGAACTCTCCAATAGAATCGATCACAAAGTCGTCTTTAGACCGCTGACCAAAGCAAACCTCGCAGACATCTTCAACCAGCAAATCAAAACCTTTCTCGCTGCTTGGAAAGAAAACGATCAAGTGAAGTTGCCAAAATTCTCCAAAGCAGAGGTCGATAAAATTATCGATAAAATCTACGACCCAGCTTTCGGTGCCAGACCCGTGGAAAGATATATCAATGATGAGGTCGAACCTCAAATCATCAATGCCCTTTTGAAATAG", "aa": "MSEFMEKFSVSKLIGSPAGYIGYDEGGGLTEAVRRKPYSVILLDEIEKASPDVLNILLQILDEGQLKDSKGRRIDFKNTIIVMTSNIGTEEFSKKKTAIGFDTGSKNDIETKQFDDIKTRVLEELKNFLTPELSNRIDHKVVFRPLTKANLADIFNQQIKTFLAAWKENDQVKLPKFSKAEVDKIIDKIYDPAFGARPVERYINDEVEPQIINALLK", "aa_hexdigest": "307d975b7e67cf67852c93fbdce3f510", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A2W4KEF2", "query_cov": 1.0, "subject_cov": 0.9774774774774775, "identity": 0.853, "score": 342.0, "evalue": 2.23e-117, "valid": false, "product": "Clp ATPase C-terminal domain-containing protein", "uniref50_id": "UniRef50_A0A2W4KEF2" }, "pscc": { "uniref50_id": "UniRef50_A0A2W4KEF2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4KEF2" ], "product": "Clp ATPase C-terminal domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_617", "locus": "GCA003260355_00581" }, { "type": "cds", "sequence": "contig_1", "start": 309531, "stop": 310184, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF17871.7", "PFAM:PF05621.17" ], "nt": "ATGAAATCTATTCTCGAAGAAGCGACCGACCCAACCAATAATATCATCCTCTTTATTGATGAGCTGCACACAATCATCGGTGCAGGAGGCCAGGACCAAAACGACGCCGCCCAAATGCTCAAGCCCTTACTTTCTAGAGGCAAAATCAAACTCATTGGTGCAACAACCTTCGATGAATACCAAAAATACATCGAAAAAGACGCCGCCCTCAAGAGAAGATTCCAGGAAGTCATAGTGAACGAGCCAAGCATCGAAATGACCAAACAAATCATCTTCGGATTGAAACCTACCTATGAAGATTTCCATGGCGTGGTAATTTCTGAGGAAGCTATTGAAAGTGCGATTATGCTCAGCAAAAGATATATCCTGAATAAACAGCTTCCTGATAAGGCACTTGATATTCTCGATGAAGCCAGTGCCAGAAAATCCACCATGCAAAAAAAGCTTGATAACGACGATGAATACAAGAAACAAGAAACCAAAATAGAAAAAATCCAGAAACAAATCGAGAAAGCGATTGAAAATCAGGATTATTTTGCTGCTGCGGAACTCAAGACCGAGGAGGAGGAATTGAAAAAAAACCTCCAGAAACTCAGATCCAATAAAAACATTCCTGCACATCTGAGAAGCGTGATCGAATCCAGCGATATCTGA", "aa": "MKSILEEATDPTNNIILFIDELHTIIGAGGQDQNDAAQMLKPLLSRGKIKLIGATTFDEYQKYIEKDAALKRRFQEVIVNEPSIEMTKQIIFGLKPTYEDFHGVVISEEAIESAIMLSKRYILNKQLPDKALDILDEASARKSTMQKKLDNDDEYKKQETKIEKIQKQIEKAIENQDYFAAAELKTEEEELKKNLQKLRSNKNIPAHLRSVIESSDI", "aa_hexdigest": "3ebc79fec1ac5078950ca2450a8afcd6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF17871.7", "name": "AAA_lid_9", "length": 104, "aa_cov": 0.4792626728110599, "hmm_cov": 0.9807692307692307, "evalue": 3.2823637560611772e-28, "score": 94.15011596679688, "start": 83, "stop": 186 }, { "id": "PF05621.17", "name": "TniB", "length": 64, "aa_cov": 0.29493087557603687, "hmm_cov": 0.984375, "evalue": 2.0043637374032786e-06, "score": 23.816316604614258, "start": 16, "stop": 79 } ], "seq_stats": { "molecular_weight": 24839.115700000024, "isoelectric_point": 5.3287351608276365 }, "id": "NKOCPLAKPK_618", "locus": "GCA003260355_00582" }, { "type": "cds", "sequence": "contig_1", "start": 310230, "stop": 310715, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3D0UX51" ], "nt": "GTGATAAGAAGTGAGGGATTCCTTGCATTTCTGGAAATAATAGATACCCTTATTGCTGAACTTAATTTAGATTATTCTGATATACAAAAAATGAATATCCAGTATGAACACACCAAAAAAGACGATGAAATCCTCGATTCTATGGAAAGCGAGATCGTAGAAAAGTCAGATTCTGACAAACTCTCCTTGGAGACCAGCGATCAAAAACCCAAAAAGAAAGAGGAAAAAAAGCTAACGATAGAATACTTCGGGACCGACCTGACGAAAGAAGCAAAAGACGGCTACCTCGACCCGATCATCGGTAGAGAAAAAGAGATCGACCAAGTGATCTATACCCTTTTGAGAAAGACCAAAAATAACCCACTCTTGATCGGTGAGGCAGGAGTCGGGAAAACCGCAATCGTTGAAGGGCTCGTGCAGAGAATCAACGCGAAACAGGTGCCTGAGAAACTCATTGGTAAAAAAGTTTTTCTCTTAGATATGTGA", "aa": "MIRSEGFLAFLEIIDTLIAELNLDYSDIQKMNIQYEHTKKDDEILDSMESEIVEKSDSDKLSLETSDQKPKKKEEKKLTIEYFGTDLTKEAKDGYLDPIIGREKEIDQVIYTLLRKTKNNPLLIGEAGVGKTAIVEGLVQRINAKQVPEKLIGKKVFLLDM", "aa_hexdigest": "4311c49bd640aaff66cb143d6c479d1c", "start_type": "GTG", "rbs_motif": "TTTTTA", "psc": { "uniref90_id": "UniRef90_A0A3D0UX51", "query_cov": 0.9751552795031055, "subject_cov": 0.9573170731707317, "identity": 0.5670000000000001, "score": 159.0, "evalue": 6.49e-47, "valid": false, "uniref50_id": "UniRef50_A0A3D0UX51" }, "pscc": { "uniref50_id": "UniRef50_A0A3D0UX51", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3D0UX51" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18377.95869999999, "isoelectric_point": 4.935125160217283 }, "id": "NKOCPLAKPK_619", "locus": "GCA003260355_00583" }, { "type": "cds", "sequence": "contig_1", "start": 310755, "stop": 311159, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGCAAACACTTGAATCTTATACCACGCAGGCGATCACTTGCTTGGACAACGCGAGCAGAATCAGCCACGCACTCCATCAAACCCGCATTACCCCAGATACGCTTTTCTGGGGCATTTATGCTTTTTTGAAACAGTCCCGCCACTTTCCCCTTTTCTGTGCAGTCATCGGACTCAAAAAGACAAAAGCCTTGGAATCCATTTTTCTCAAGGAGATTCAAGTCCCAATGCTTCAGTCCGAGACGAAGCTCCTCCCCTTCAATAAAATCCTTGCTTCCGATTGCTTGAGCAAAATTGAAAAGGGACAAAAAAACATTGATCCTTTCCAGCTCTTTATCCTTGCATTTGAGAGACTCAGCCCAAAAATCAAAAAGCTGATCCAAAAGGACAAGAATAACCTCTGA", "aa": "MKQTLESYTTQAITCLDNASRISHALHQTRITPDTLFWGIYAFLKQSRHFPLFCAVIGLKKTKALESIFLKEIQVPMLQSETKLLPFNKILASDCLSKIEKGQKNIDPFQLFILAFERLSPKIKKLIQKDKNNL", "aa_hexdigest": "c2596c4de9432e97de7e67b2dcadcfd2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15423.075099999985, "isoelectric_point": 9.647580909729005 }, "id": "NKOCPLAKPK_620", "locus": "GCA003260355_00584" }, { "type": "cds", "sequence": "contig_1", "start": 311222, "stop": 311410, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBFC355", "UniRef:UniRef100_A0A328EK78", "UniRef:UniRef50_A0A660MNY5", "UniRef:UniRef90_A0A328EK78" ], "nt": "ATGCAAGAGAAAAAGTCGGAGAAGGAGTCGCGGAGTTTGGATATTTTGCTAAGATTTGTGATGTTTGCGAGAGTAAATTGGATTGATTCGTCCCTGATCAGCTCTTCCCTCAACAAACGGATCGAAGAAAAGAGCGATATGCTTGATAAAGATTCTTTTTTCTCTGTAGGTAATCCCTCTGTCGATTAA", "aa": "MQEKKSEKESRSLDILLRFVMFARVNWIDSSLISSSLNKRIEEKSDMLDKDSFFSVGNPSVD", "aa_hexdigest": "8d4e91bf1b44fd3695325f2e0932651d", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000DBFC355", "uniref100_id": "UniRef100_A0A328EK78", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBFC355", "UniRef:UniRef100_A0A328EK78" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EK78", "uniref90_id": "UniRef90_A0A328EK78" }, "psc": { "uniref90_id": "UniRef90_A0A328EK78", "uniref50_id": "UniRef50_A0A660MNY5" }, "pscc": { "uniref50_id": "UniRef50_A0A660MNY5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MNY5" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7185.0884, "isoelectric_point": 5.331292915344237 }, "id": "NKOCPLAKPK_621", "locus": "GCA003260355_00585" }, { "type": "cds", "sequence": "contig_1", "start": 311775, "stop": 312035, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTGCGATGGATCCTGTGGCTACGAGATGGATTATGGCGGTTATGAGTCCTCTTCCTAAGAGAATGCCTGTGGTAATGTGCTCTCCCTGTGTAAGCAAAGTGCCGAGTGCAAAGAGATTTTCGATTACACTGAAAGAAAAGCTTGCAAGTAATGAGAAGAGTAAGAGGTTTGAGGGAGATTGGAGGAGAGAGGCTTCGGATTGGTTGTGGCTGAGGGTGAATTTGAAGTTTTCCTCGGTAGCGGCTGCGAGTATGTAG", "aa": "MSAMDPVATRWIMAVMSPLPKRMPVVMCSPCVSKVPSAKRFSITLKEKLASNEKSKRFEGDWRREASDWLWLRVNLKFSSVAAASM", "aa_hexdigest": "98d51ec2002fdbfa986cbd9b2f9dc07d", "start_type": "ATG", "rbs_motif": "TTTTT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9762.523599999995, "isoelectric_point": 10.221671867370606 }, "id": "NKOCPLAKPK_622", "locus": "GCA003260355_00586" }, { "type": "cds", "sequence": "contig_1", "start": 312119, "stop": 312430, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCAAATCATTAGTCTCATTCTTAGCATAGGAATAATTATTTGGTTCTGGGCTTGGTTCTTAAACCAAGTTTTTTCTCGTGCTTCGAAATCAGGCTACAAACTACGAAAATCTCTCCTCCAATGTGGGATTCTGGTTTTTCTACTTTTTATCTATGAAAAAGCACTAACGCTCCTCGGATTCAATGCCAGCTTCATCTTTTCACACGAAAGCCTAACGCTACCCTCAATCCTCCTCTTTCTTATCTACAGTTTCCTTATAGTAATAGGGATTGCCCTCCTCTTTGGACAGTCAAAAAACAAAAAAATCTGA", "aa": "MQIISLILSIGIIIWFWAWFLNQVFSRASKSGYKLRKSLLQCGILVFLLFIYEKALTLLGFNASFIFSHESLTLPSILLFLIYSFLIVIGIALLFGQSKNKKI", "aa_hexdigest": "2e656ea9b2ac9b33b25b63a38433e46d", "start_type": "GTG", "rbs_motif": "TTTA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11792.199199999981, "isoelectric_point": 10.012020683288572 }, "id": "NKOCPLAKPK_623", "locus": "GCA003260355_00587" }, { "type": "cds", "sequence": "contig_1", "start": 312624, "stop": 312734, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGAATCGTTCAAAAAAGTAAAAACTGAACACACCATTCTGTCTGAATTTCTTCCTTTTTTAACGAAGTTAGAACAAGAGCCGGGAATCCAGAGAATGATTCCCTGA", "aa": "MKESFKKVKTEHTILSEFLPFLTKLEQEPGIQRMIP", "aa_hexdigest": "b996acd07d436da0d94d0ee5c672799b", "start_type": "ATG", "rbs_motif": "TTTA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4275.041000000001, "isoelectric_point": 8.162745094299318 }, "id": "NKOCPLAKPK_624", "locus": "GCA003260355_00588" }, { "type": "cds", "sequence": "contig_1", "start": 312792, "stop": 313076, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EG80", "UniRef:UniRef90_A0A328EG80" ], "nt": "TTGAATGCTCTAAAAGCGGAAGGAAAAGAAGTAAGACTACAATATTCTTACACCGAAAAAGGAGATGGACATCGCTTCGTAGTAGAAAAAAAGGAAGGAGGAGAAGAAACTATTCTTGATCCAACCTACCTACAATACGATAAGAACTATCCTGAAGGATTTGTCGGGCAAAGCTTTCCAGACCAAAAGCTCGAAAAGAATAGAACTGAAGAAAAAGACTTTATGGAGCTCCAGAAAAAACGATACGAAGAAGGAGTTTATGATAAGTTTTTCGCTAAAAAATAA", "aa": "MNALKAEGKEVRLQYSYTEKGDGHRFVVEKKEGGEETILDPTYLQYDKNYPEGFVGQSFPDQKLEKNRTEEKDFMELQKKRYEEGVYDKFFAKK", "aa_hexdigest": "da578daed63140152a9757ec3a799736", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 312792, "stop": 313190, "observations": { "mutation": [ 313118 ], "stop": [ 313118 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EG80", "query_cov": 1.0, "subject_cov": 0.7121212121212122, "identity": 0.9890000000000001, "score": 189.0, "evalue": 2.25e-60, "gene_start": 1, "gene_end": 94, "reference_start": 39, "reference_end": 132, "reference_sequence": "MNLNKKNNSEEIMHSIIKKLSTQPGFPNDYCNIASKALLNALKAEGKEVRLQYSYTEKGDGHRFVVEKKEGGEETILDPTYLQYDKNYPEGFVGQSFPDQKLEKNRTEEKDFMELQKKRYEEGVYDKFFAKK" }, "paralog": false, "identity": 0.9924242424242424, "score": 269.0, "evalue": 2.42e-94, "description": "Internal stop codon at 313118. Nonsense mutation around 313118.", "psc": { "uniref90_id": "UniRef90_A0A328EG80", "uniref50_id": "UniRef50_A0A328EG80", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EG80", "UniRef:UniRef50_A0A328EG80" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EG80", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EG80" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 11170.349099999994, "isoelectric_point": 5.524602317810058 }, "id": "NKOCPLAKPK_625", "locus": "GCA003260355_00589" }, { "type": "cds", "sequence": "contig_1", "start": 313317, "stop": 313538, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF17827.6" ], "nt": "ATGCAGATTCAATCATTTATTCAGGATAGTGCTTTTAAGAAAAAATTTGTGCTGGAGAAACTTCTTTGTCATTATCTCAATAAGACAAGATCAGAGCTGCGGACTGATGGGAATCAGGAAATTCCTCCTACTATAGAGCAGCAAATTAGGGCTGATTATCGTGCGTATGAGGAAGATGATAAACCGCTTGAGTATTTACTCGGGTTCGTGGAATTTTTTTGA", "aa": "MQIQSFIQDSAFKKKFVLEKLLCHYLNKTRSELRTDGNQEIPPTIEQQIRADYRAYEEDDKPLEYLLGFVEFF", "aa_hexdigest": "aacf7c9c6a6430add372550a168f8c6d", "start_type": "ATG", "rbs_motif": "TTATT", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF17827.6", "name": "PrmC_N", "length": 51, "aa_cov": 0.6986301369863014, "hmm_cov": 0.9803921568627451, "evalue": 3.547968006561012e-08, "score": 30.37458610534668, "start": 18, "stop": 68 } ], "seq_stats": { "molecular_weight": 8776.871399999998, "isoelectric_point": 5.044028663635253 }, "id": "NKOCPLAKPK_626", "locus": "GCA003260355_00590" }, { "type": "cds", "sequence": "contig_1", "start": 313674, "stop": 314108, "strand": "+", "frame": 3, "gene": null, "product": "peptide chain release factor N(5)-glutamine methyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A288DK12", "UniRef:UniRef90_A0A660MQG3" ], "nt": "GTGCTTGGATGTGCGGTTTTGCTCCAGAATGAGGGGAAATTTTGTGATGTTTTTTTCTCAGATATTTCCTCAGAAGCACTTACTGTTGCCAAAAAAAATTATGACACGCTAATCGGTGATCCGTCTTATAACCTTAGGATTCTTCAGTCAGATTTGTGTGCTTTTTTAGAAGACTATCAGGAGATTATCAGAGATAAGGATCTTGTTTTAACTGCGAATTTGCCGTATATCCCTGACAAAACCTTTGAAGATAACGCTCCTCGTAATGTGCAAAAGTGGGAGCCTAAGGTTGCTTTTGTGGGTGGAGAAGATGGATTGGACTATTATAGGAGGATGTTTGAGCAACTTCAAATCCTTGCAAAACCTGCGAAAACTTTGGTGATGTTTCTTGAAATGATGACTTGGCAATTGGAAATTTTAAGGGCAGAATTCTGA", "aa": "MLGCAVLLQNEGKFCDVFFSDISSEALTVAKKNYDTLIGDPSYNLRILQSDLCAFLEDYQEIIRDKDLVLTANLPYIPDKTFEDNAPRNVQKWEPKVAFVGGEDGLDYYRRMFEQLQILAKPAKTLVMFLEMMTWQLEILRAEF", "aa_hexdigest": "f7f8664a2876e27e4ef2d42a09232191", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 313374, "stop": 314180, "observations": { "mutation": [ 313536, 313659, 314106 ], "stop": [ 313536, 313659, 314106 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A660MQG3", "query_cov": 1.0, "subject_cov": 0.5017421602787456, "identity": 0.938, "score": 271.0, "evalue": 1.07e-89, "gene_start": 1, "gene_end": 144, "reference_start": 120, "reference_end": 263, "reference_sequence": "MQIQSFIQDSAFKKKFVLEKLLCHYLNKTRSELRTDGEQEIPAELEQQIRADYRAYEEDDKPLEYLLGFVEFFGIKFFVNQDTLVPRPETEYMITAISEYLYEKSQTDMVLLDIGAGCGVLGCAVLLQNEGKFRDVFFSDISSEALAVVKKNYDTLIVDQPYNLRILQSDLCAFLEDYQEIIRDKDIVLTANLPYIPDKTFEDNAPRNVQKWEPKVAFVGGEDGLDYYRRMFEQLQILAKPAKTLVMFLEMMTWQLEILREEFGSILEFEEVKTFHFNIRIVKASFK" }, "paralog": false, "identity": 0.9328358208955224, "score": 499.0, "evalue": 5.77e-182, "description": "Internal stop codon at 313536, 313659, 314106. Nonsense mutation around 313536, 313659, 314106.", "psc": { "uniref90_id": "UniRef90_A0A660MQG3", "product": "peptide chain release factor N(5)-glutamine methyltransferase", "uniref50_id": "UniRef50_A0A288DK12", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A660MQG3", "UniRef:UniRef50_A0A288DK12" ] }, "pscc": { "uniref50_id": "UniRef50_A0A288DK12", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A288DK12" ], "product": "peptide chain release factor N(5)-glutamine methyltransferase" } }, "id": "NKOCPLAKPK_627", "locus": "GCA003260355_00591" }, { "type": "cds", "sequence": "contig_1", "start": 314251, "stop": 314607, "strand": "+", "frame": 1, "gene": null, "product": "Alpha/beta hydrolase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EI48", "UniRef:UniRef90_A0A328EI48" ], "nt": "TTGATAAAAGATAATGATGAACTTCTAAAAGTCATGCAAACTTGGGAAGTAAATCCTTTTGAAGAAAAAAAGAGACGAAGACTGGTTTTACAGGAGAGGTTGCCTGAATTTTTCGTTATAAAACCTGAAATGCCAAATAAAGATATGGCACGTTATAGCGCTTGGAAGCTTCGGTTTGAGAAGTATTTTCAGTTTTTGGATGATGAAGAGTTGATTTTGATTGGGCATTCGCTTGGAGCGATGTTTTTGGTGAAGTATTTTGCTGAAAATCAGTTTCCAAAAAAGGTAGCTCAGTTGCATTTGGTAGCTCCTGTGCTGGATGCTGAGGGATTACCAGAGGGGGATAATTATTTATGA", "aa": "MIKDNDELLKVMQTWEVNPFEEKKRRRLVLQERLPEFFVIKPEMPNKDMARYSAWKLRFEKYFQFLDDEELILIGHSLGAMFLVKYFAENQFPKKVAQLHLVAPVLDAEGLPEGDNYL", "aa_hexdigest": "1dd3be5dadc6842b2f14d61b875eaecc", "start_type": "TTG", "rbs_motif": "TTATT", "genes": [], "truncated": "both", "pseudogene": { "start": 314218, "stop": 314823, "observations": { "mutation": [ 314245, 314605 ], "stop": [ 314245, 314605 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EI48", "query_cov": 1.0, "subject_cov": 0.5870646766169154, "identity": 0.975, "score": 235.0, "evalue": 3.05e-77, "gene_start": 1, "gene_end": 118, "reference_start": 12, "reference_end": 129, "reference_sequence": "MRQIVFIHGGNLIKDNDELLKVMQTWEVNPFEEKKRRRLVLQERLPEFFVIKPEMPNKDMARYSTWKLWFEKYFQFLDDEELILIGHSLGAMFLVKYFAENQFPKKVAQLHLVAPVLDAEGLPEGDNYLGDFAYDFAAVKNISSKAEKVVIWSSKDDPTVPYQHSVRIQSQIVGSQLRMFEDRGHFNQAEFLELIEEIRKK" }, "paralog": false, "identity": 0.9502487562189055, "score": 386.0, "evalue": 2.68e-139, "description": "Internal stop codon at 314245, 314605. Nonsense mutation around 314245, 314605.", "psc": { "uniref90_id": "UniRef90_A0A328EI48", "product": "Alpha/beta hydrolase", "uniref50_id": "UniRef50_A0A328EI48", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EI48", "UniRef:UniRef50_A0A328EI48" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EI48", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EI48" ], "product": "Alpha/beta hydrolase" } }, "id": "NKOCPLAKPK_628", "locus": "GCA003260355_00592" }, { "type": "cds", "sequence": "contig_1", "start": 314632, "stop": 314823, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAAAAATATCTCATCAAAAGCAGAGAAAGTCGTGATTTGGAGTTCAAAGGATGATCCAACTGTTCCTTATGAGCATAGTGTGCGTATTCAGTCGCAGATTGTTGGTTCTCAGCTGAGAATGTTTGAAGATAAGGGACATTTTAATCAGGCGGAGTTCCCTGAGTTGATCCAAGAAATTAGAAAAAAATAG", "aa": "MKNISSKAEKVVIWSSKDDPTVPYEHSVRIQSQIVGSQLRMFEDKGHFNQAEFPELIQEIRKK", "aa_hexdigest": "3897a3387a1f8ef52e033651ec68096a", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7359.3157999999985, "isoelectric_point": 8.077324485778806 }, "id": "NKOCPLAKPK_629", "locus": "GCA003260355_00593" }, { "type": "cds", "sequence": "contig_1", "start": 315158, "stop": 315433, "strand": "+", "frame": 2, "gene": null, "product": "DNA-binding protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBFED92", "UniRef:UniRef100_A0A328EGG4", "UniRef:UniRef50_A0A328EGG4", "UniRef:UniRef90_A0A328EGG4" ], "nt": "ATGACAAAGACAGCACTTATTGCTAGTTTGGCAAACGAACTTGGAGTATCTAAGAAGCTTGCTGCTGAATTAGTAAATGCTTTTATTGAAACTGTGATTACTGGTGTTAAGAAACAGGGAGAAGTTAGAGTTCAAGGATTTGGAACTTTTAAGGCTTCTAAGAGAAAGGCTAGAGAAGGAGTTAATCCTCAGAATCCTAAGCAGAAGATTAAGATCCCTGCAATGAAGGTTGTTACTTTCAAGGCTGGTTCTGACTTCAAAGCTGCTGTGAAATAA", "aa": "MTKTALIASLANELGVSKKLAAELVNAFIETVITGVKKQGEVRVQGFGTFKASKRKAREGVNPQNPKQKIKIPAMKVVTFKAGSDFKAAVK", "aa_hexdigest": "c597164bd588117e8cd53729dcbdd40c", "start_type": "ATG", "rbs_motif": "TTATT", "ups": { "uniparc_id": "UPI000DBFED92", "uniref100_id": "UniRef100_A0A328EGG4", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBFED92", "UniRef:UniRef100_A0A328EGG4" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EGG4", "uniref90_id": "UniRef90_A0A328EGG4" }, "psc": { "uniref90_id": "UniRef90_A0A328EGG4", "product": "DNA-binding protein", "uniref50_id": "UniRef50_A0A328EGG4" }, "pscc": { "uniref50_id": "UniRef50_A0A328EGG4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EGG4" ], "product": "DNA-binding protein" }, "genes": [], "id": "NKOCPLAKPK_630", "locus": "GCA003260355_00594" }, { "type": "cds", "sequence": "contig_1", "start": 315609, "stop": 315878, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF09180.16" ], "nt": "ATGGAAAAAAAACTCGTGAAGCTTGAAAATCTCGCAGAAGAAATTCAGACTTTGCTTCTTGAAATTCAAGAAAAAATGCTCGCAAAACATCAGCTTTTCACTTTGGAACATACCTTTACTGCAGATACTTATGAGGAACTTAAAGAAAAAGTAGAACAAGGTTTCGTGCTAGCTCATTGGGATGGAACGAAGGAAACTGCGATGAAAGTGCAAGAGGAACTTAAAGCAACGATTAGATGTCTGCCCTTTGATATGCCTGAGGAAGCTTGA", "aa": "MEKKLVKLENLAEEIQTLLLEIQEKMLAKHQLFTLEHTFTADTYEELKEKVEQGFVLAHWDGTKETAMKVQEELKATIRCLPFDMPEEA", "aa_hexdigest": "b380fcd311f7fff9f25c2b815745f7c2", "start_type": "ATG", "rbs_motif": "TTTTTA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF09180.16", "name": "ProRS-C_1", "length": 44, "aa_cov": 0.48314606741573035, "hmm_cov": 1.0, "evalue": 2.6625948099079087e-14, "score": 49.58763885498047, "start": 44, "stop": 86 } ], "seq_stats": { "molecular_weight": 10420.93699999999, "isoelectric_point": 4.8276994705200185 }, "id": "NKOCPLAKPK_631", "locus": "GCA003260355_00595" }, { "type": "cds", "sequence": "contig_1", "start": 315906, "stop": 316016, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03129.25" ], "nt": "GTGAAGATTGACGATGACGATCAGAAATCCCCAGGACGAAAATTCAATGAATGGGAACTCAAAGGAGTGCCAATTAGAATCGCTGTTGGGAAGAGAGATTTGGAACAGTGA", "aa": "MKIDDDDQKSPGRKFNEWELKGVPIRIAVGKRDLEQ", "aa_hexdigest": "7bc56ec2cba7be0561864a7e73c89c30", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03129.25", "name": "HGTP_anticodon", "length": 33, "aa_cov": 0.9166666666666666, "hmm_cov": 0.9696969696969697, "evalue": 1.0644912106745878e-09, "score": 34.86043930053711, "start": 3, "stop": 35 } ], "seq_stats": { "molecular_weight": 4212.743100000001, "isoelectric_point": 6.100438117980955 }, "id": "NKOCPLAKPK_632", "locus": "GCA003260355_00596" }, { "type": "cds", "sequence": "contig_1", "start": 316038, "stop": 317069, "strand": "-", "frame": 2, "gene": "proS", "product": "proline--tRNA ligase", "db_xrefs": [ "EC:6.1.1.15", "GO:0004827", "GO:0005524", "GO:0005737", "GO:0006433", "KEGG:K01881", "SO:0001217", "UniRef:UniRef50_Q8A988", "UniRef:UniRef90_A0A328EJF4" ], "nt": "ATGAATACCATGGCAACGAAATTGACTTCAAGAGCTGAGAACTACTCTGAACGATATAATGATTTAGTAAAATACGCAGATTTAGCTGAAAACTCCTCTGTAAGAGGGTGTATGGTAATTAAACCCTATGGATATGCGATCTGGGAAAATATTAGAGATGTACTTGATGCAATGTTTAAGGCAACCGGGCATCAGAATGCCTATTTCCCACTCTTTATCCCAAAATCTTTTTTTAGCAAGGAAGCGAATCATGTAGAGGGATTTGCCAAAGAATGTGCGGTAGTAACTCACTATAGGCTGAAAAATGATGAAAATGGCAAAGGGATTGTCGTAGATCCTGATGCAAAACTCGAGGAAGAGTTGATTGTCAGACCGACTTCAGAAACGATTATCCGAGATAGTTATAGAAATTGGATCAAGTCACATCGTGATTTACCACTCCTTGTAAATCAATGGGCAAATGTGGTGAGATGGGAGATGAGAACGAGAATTTTCCTCAGGAGTGCAGAATTTCTCCGGCAGGAAGGGCACACTGCTCATGCTACGGCTGAAGAAGCTGATATTGAAGCGAGAAAAATGATGGAAGTGTATTCGGATTTTGTTTCTCATTTTATGGCAGTGCATCATTATAAAGGAGAGAAACCTGAACATGAAAAGTTTGCAGGGGCGGTTAAGACCTATACTTTTGAACCAATGATGCAGGATTTTAAAGCACTTCAGGCGGGAACTTCCCACAACCTCGGTCAAAACTTTGCAAAAGCCTTTGATGTAAAATTCACCAATAAAAATAACGAACTTGAGTATGTGTATGCGACTTCTTGGGGAGTTACTACAAGACTTCTTGGTTGTCTGATTATGACACATTCTGATGATCATGGATTGGTCTTGCCACCTGCACTCGCACCGCTTCATCTCGTGGTAGTTCCTATTTTCAAAAATACAGAAGATTTGGATCAGATTTTAGGTGCTTTGCAACCTGCTTTGAATCAGCTCAAAGCTTCAAAATTACAAATAAAGTCTGATTTCCTCTGA", "aa": "MNTMATKLTSRAENYSERYNDLVKYADLAENSSVRGCMVIKPYGYAIWENIRDVLDAMFKATGHQNAYFPLFIPKSFFSKEANHVEGFAKECAVVTHYRLKNDENGKGIVVDPDAKLEEELIVRPTSETIIRDSYRNWIKSHRDLPLLVNQWANVVRWEMRTRIFLRSAEFLRQEGHTAHATAEEADIEARKMMEVYSDFVSHFMAVHHYKGEKPEHEKFAGAVKTYTFEPMMQDFKALQAGTSHNLGQNFAKAFDVKFTNKNNELEYVYATSWGVTTRLLGCLIMTHSDDHGLVLPPALAPLHLVVVPIFKNTEDLDQILGALQPALNQLKASKLQIKSDFL", "aa_hexdigest": "57b885a25dc42fc2382bc10b306602cb", "start_type": "ATG", "rbs_motif": "TTATT", "psc": { "uniref90_id": "UniRef90_A0A5J4S6B4", "query_cov": 0.9737609329446064, "subject_cov": 0.9570200573065902, "identity": 0.701, "score": 471.0, "evalue": 2.03e-164, "valid": false, "product": "Proline--tRNA ligase", "ec_ids": [ "6.1.1.15" ], "uniref50_id": "UniRef50_A0A958NEB7", "kegg_orthology_id": "K01881" }, "genes": [ "proS" ], "truncated": "5-prime", "pseudogene": { "start": 315735, "stop": 317069, "observations": { "mutation": [ 315908, 316040 ], "stop": [ 315908, 316040 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EJF4", "query_cov": 1.0, "subject_cov": 0.6778656126482213, "identity": 1.0, "score": 691.0, "evalue": 5.24e-249, "gene_start": 1, "gene_end": 343, "reference_start": 1, "reference_end": 343, "reference_sequence": "MNTMATKLTSRAENYSERYNDLVKYADLAENSSVRGCMVIKPYGYAIWENIRDVLDAMFKATGHQNAYFPLFIPKSFFSKEANHVEGFAKECAVVTHYRLKNDENGKGIVVDPDAKLEEELIVRPTSETIIRDSYRNWIKSHRDLPLLVNQWANVVRWEMRTRIFLRSAEFLRQEGHTAHATAEEADIEARKMMEVYSDFVSHFMAVHHYKGEKPEHEKFAGAVKTYTFEPMMQDFKALQAGTSHNLGQNFAKAFDVKFTNKNNELEYVYATSWGVTTRLLGCLIMTHSDDHGLVLPPALAPLHLVVVPIFKNTEDLDQILGALQPALNQLKASKLQIKSDFLGTYDLPYKVKIDDDDQKSPGRKFNEWELKGVPIRIAVGKRDLEQGQVELFRRDTMEKKLVKLENLAEEIQTLLLEIQEKMLAKHQLFTLEHTFTADTYEELKEKVEQGFVLAHWDGTKETAMKVQEELKATIRCLPFDMPEEAGIDPVSGKPSARRVIYAKSY" }, "paralog": false, "identity": 0.9954954954954955, "score": 890.0, "evalue": 0.0, "description": "Internal stop codon at 315908, 316040. Nonsense mutation around 315908, 316040.", "psc": { "uniref90_id": "UniRef90_A0A328EJF4", "gene": "proS", "product": "proline--tRNA ligase", "ec_ids": [ "6.1.1.15" ], "uniref50_id": "UniRef50_Q8A988", "kegg_orthology_id": "K01881", "go_ids": [ "GO:0004827", "GO:0005524", "GO:0005737", "GO:0006433" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EJF4", "EC:6.1.1.15", "UniRef:UniRef50_Q8A988", "KEGG:K01881", "GO:0004827", "GO:0005524", "GO:0005737", "GO:0006433" ] }, "pscc": { "uniref50_id": "UniRef50_Q8A988", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q8A988" ], "product": "Proline--tRNA ligase" } }, "id": "NKOCPLAKPK_633", "locus": "GCA003260355_00597" }, { "type": "cds", "sequence": "contig_1", "start": 317434, "stop": 318216, "strand": "+", "frame": 1, "gene": null, "product": "Trigger factor", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EJN6", "UniRef:UniRef90_A0A328EJN6" ], "nt": "GTGAAGAATCATGTGCTTGAGCATTTTGCAAAAGATATGAATATCCCTGGATTTAGAGCGGGGAAAGTTCCTCTTCATATGGTAGAATCTAAAGTGCAACCTGCCTATGTTCAGATGGCGATTACCGAACACTTGGTAAATAAGGGAATCCAGGAGCTTCTTGGAGAAAATAAAGATTTGAAATTTATTGGTGAACCGTATGCGTTTGATACCAAAGAAGACAAGGGAACTACGACAGTTTCTTTTTCGCTTGATGTGTATCCGGAGGTAGAAGTGAAGGGAAAGTCTTGGGAAAAAGTGCAGATGAATGCCTTAAGTGTTGAGGCAACAGAAAAAGAAGTAGAGGATTCTCTCCTTAATATCCAGAAAAATTATGCTGACTACAAAGATACCGATACGATTGCTGTCAAAGATACCCTTTCCAAGCTCGGACTTGAATTTTTTGATAAGGAAGGAAAGAGTGTAGAAAAAGGAACGACCTATCTCGGAGAAACTGAATTTGCTGAAGATAAGTTCTGGGAGAAGACTTTTGATGGAAAAAAGAAAGGAGAGCTTGTAGAACTCAAATATGATGTGAAAAAACTTCCTGCCGTTTTGCATGCCAAAAAAGGTGATGCTGCCAGTCTAAAAATTACCGTTCAAGATGTGAAGCAAATCGTGCTTCCTGAGCTTAATGATGAAATGATTGTGAAACTTTTTGGAAAAGATGCTGAGGTAAAGACGAATGCTGAACTTAGAGAGTATATCAAGAAAGAAATCTTGAAACAAAAACAGGAAAACTGA", "aa": "MKNHVLEHFAKDMNIPGFRAGKVPLHMVESKVQPAYVQMAITEHLVNKGIQELLGENKDLKFIGEPYAFDTKEDKGTTTVSFSLDVYPEVEVKGKSWEKVQMNALSVEATEKEVEDSLLNIQKNYADYKDTDTIAVKDTLSKLGLEFFDKEGKSVEKGTTYLGETEFAEDKFWEKTFDGKKKGELVELKYDVKKLPAVLHAKKGDAASLKITVQDVKQIVLPELNDEMIVKLFGKDAEVKTNAELREYIKKEILKQKQEN", "aa_hexdigest": "2e6c5a8bbb7de8af25f4f61db9eccb5c", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 317356, "stop": 318519, "observations": { "mutation": [ 317377, 318214 ], "stop": [ 317377, 318214 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EJN6", "query_cov": 1.0, "subject_cov": 0.6132075471698113, "identity": 0.996, "score": 499.0, "evalue": 1.21e-175, "gene_start": 1, "gene_end": 260, "reference_start": 27, "reference_end": 286, "reference_sequence": "MEKQLTQGKQCNYQVTFTFTDAEKAGVKNHVLEHFAKDMNIPGFRAGKVPLHMVESKVQPAYVQMAITEHLVNKGIQELLGENKDLKFIGEPYAFDTKEDKGTTTVSFSLDVYPEVEVKGKSWEKVQMNALSVEATEKEVEDSLLNIQKNYADYKDTDTIAVKDTLSKLGLEFFDKEGKSVEKGTTYLGETEFAEDKFWEKTFDGKKKGELVELKYDVKKLPAVLHAKKGDAASLKITVQDVKQIVLPELNDEMIVKLFGKDAEVKTNAELREYIKKEILKQKQENGLVQTIEDYLNEVKKAGVSVIIPKTMVDQELKVRMQNLEKRFGSAEKVKEYFQQLGEEKAKEFVEGVQSAAAESLEKFFILNKVTELLGLEIDWNSEQEPFFVEKQLYTKLVGELETPADEKKATKKAPAKKTTKKAE" }, "paralog": false, "identity": 0.9948320413436692, "score": 754.0, "evalue": 9.47e-279, "description": "Internal stop codon at 317377, 318214. Nonsense mutation around 317377, 318214.", "psc": { "uniref90_id": "UniRef90_A0A328EJN6", "product": "Trigger factor", "uniref50_id": "UniRef50_A0A328EJN6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EJN6", "UniRef:UniRef50_A0A328EJN6" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EJN6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EJN6" ], "product": "Trigger factor" } }, "id": "NKOCPLAKPK_634", "locus": "GCA003260355_00598" }, { "type": "cds", "sequence": "contig_1", "start": 318241, "stop": 318630, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATGAGGTAAAGAAGGCTGGAGTAAGTGTAATTATCCCAAAGACGATGGTAGATCAAGAACTTAAAGTCAGAATGCAGAATCTTGAAAAGAGATTTGGTTCTGCTGAAAAGGTAAAAGAATATTTCCAGCAGCTTGGAGAAGAAAAGGCGAAAGAATTTGTAGAAGGAGTGCAGAGTGCAGCTGCAGAGAGCCTTGAAAAGTTCTTTATTCTCAATAAAGTTACCGAGCTTCTTGGATTGGAAATTGATTGGAATAGTGAACAGGAACCTTTCTTCGTAGAAAAGCAGCTCTATACTAAATTAGTAGGGGAGTTGGAGACTCCAGCTGATGAGAAGAAAGCAACTAAAAAAGCGCCTGCCAAAAAAACTACTAAAAAAGCTGAATAA", "aa": "MNEVKKAGVSVIIPKTMVDQELKVRMQNLEKRFGSAEKVKEYFQQLGEEKAKEFVEGVQSAAAESLEKFFILNKVTELLGLEIDWNSEQEPFFVEKQLYTKLVGELETPADEKKATKKAPAKKTTKKAE", "aa_hexdigest": "83e9a6634deb59cbc5382375f647d217", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14682.767199999987, "isoelectric_point": 6.453351402282714 }, "id": "NKOCPLAKPK_635", "locus": "GCA003260355_00599" }, { "type": "cds", "sequence": "contig_1", "start": 319017, "stop": 319661, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EK87", "UniRef:UniRef90_A0A328EK87" ], "nt": "ATGTTTCACAAGGCTTATGCTTTTGATATTGATGATAATTTGCTTCATACGAATTGCACGGTGTTTGTAGAAAAGTGGGATGGGAGCAAGATGCAGAGGTGCGAAATCCCGGAAAGTGAGCTTAAGGCAAAACTCAAAGAGGGGCGAACTTACCCAAAGAATGATCCGGAAGAGTGTTATTATAATATGAGGCTTGGCGGAGGGAAGCTTACCCAAGATCTTTTTGATGCACTAGATGCGAAAGCTTATGGACCATCGTGGGAGAGGTTTAAACTTGCTAATCTGCAGGCGAGTCCTATAGGAATCATCACTGCTCGTGGACATCCGATTGAGGATCTTAGGAGTGCTCATCAAGCTCTTTTGCATGAGGCGTTTACGCTTGAAGAAAGAGAACAATTTTTGGAAAAGATGAAGCTTCGTTTGGGAGAGCATAGAGCGAGCTCAGATCATCTTATTCGCGATTTTTTTGATATGAACTATTATGCTGCTTGTGCTAATAAAGACTATTCGGATAGTCTTCAGATCCCTTTTGATACTCCTTCTTCAAAGAAGAAAGTTTATGCTTTTGATAAATTTATTGAGCATATCGTGGCGCTCAATCAACACGGAAAGATCAATCCCCATCAAAAAGCTCTAAAAATCTGA", "aa": "MFHKAYAFDIDDNLLHTNCTVFVEKWDGSKMQRCEIPESELKAKLKEGRTYPKNDPEECYYNMRLGGGKLTQDLFDALDAKAYGPSWERFKLANLQASPIGIITARGHPIEDLRSAHQALLHEAFTLEEREQFLEKMKLRLGEHRASSDHLIRDFFDMNYYAACANKDYSDSLQIPFDTPSSKKKVYAFDKFIEHIVALNQHGKINPHQKALKI", "aa_hexdigest": "e7b6e28682345fb47afb0d964e0f7bce", "start_type": "ATG", "rbs_motif": "TTATT", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 319017, "stop": 319829, "observations": { "mutation": [ 319659 ], "stop": [ 319659 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EK87", "query_cov": 1.0, "subject_cov": 0.7925925925925926, "identity": 1.0, "score": 443.0, "evalue": 9.24e-157, "gene_start": 1, "gene_end": 214, "reference_start": 1, "reference_end": 214, "reference_sequence": "MFHKAYAFDIDDNLLHTNCTVFVEKWDGSKMQRCEIPESELKAKLKEGRTYPKNDPEECYYNMRLGGGKLTQDLFDALDAKAYGPSWERFKLANLQASPIGIITARGHPIEDLRSAHQALLHEAFTLEEREQFLEKMKLRLGEHRASSDHLIRDFFDMNYYAACANKDYSDSLQIPFDTPSSKKKVYAFDKFIEHIVALNQHGKINPHQKALKIGFSDDSLENLSAVEEAIQNTFVLSYPEITFALYNTNNPRFVKKTLYASAQNKEPEF" }, "paralog": false, "identity": 0.9962962962962963, "score": 551.0, "evalue": 9.04e-202, "description": "Internal stop codon at 319659. Nonsense mutation around 319659.", "psc": { "uniref90_id": "UniRef90_A0A328EK87", "uniref50_id": "UniRef50_A0A328EK87", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EK87", "UniRef:UniRef50_A0A328EK87" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EK87", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EK87" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 24740.83880000001, "isoelectric_point": 6.552535438537597 }, "id": "NKOCPLAKPK_636", "locus": "GCA003260355_00600" }, { "type": "cds", "sequence": "contig_1", "start": 319695, "stop": 319829, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGAGGAGGCGATTCAAAACACCTTTGTGCTTAGCTATCCGGAAATTACCTTTGCACTCTACAATACCAACAATCCTCGCTTCGTAAAAAAGACGCTCTATGCTTCCGCACAAAATAAAGAACCAGAATTTTAG", "aa": "MEEAIQNTFVLSYPEITFALYNTNNPRFVKKTLYASAQNKEPEF", "aa_hexdigest": "c9f3918e314ae6773945b6d81e4ad17d", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5158.747500000001, "isoelectric_point": 5.062103462219238 }, "id": "NKOCPLAKPK_637", "locus": "GCA003260355_00601" }, { "type": "cds", "sequence": "contig_1", "start": 320653, "stop": 321264, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EGF9", "UniRef:UniRef90_A0A328EGF9" ], "nt": "ATGAAAAAATGGATAAAAAAACGGATGTGGTGGGTTTTGGTTATTCTCCTGCTCGTTCCTATCTTTTTTAAGACGGGTATTATTCTTTATAATAGGATAGATGCTTCATCAGAAAATTGGAAGTACTTCTCTTGTAAGTTGACGGGGAAAGAACAAACCATGATTAACTGTAGCGATAAGAATAAAGATTTTATTATTTCTGGAGATCAAGTGTCGTTTGCAGGGCGTGGATCATGGAATTCAGTCTATATTCCTTTCTCTTCTGACCGCATTACACTTCGTATTGATCCTGAAACGAGAGCTGGGTATGATTTTTGGGTTTTGTATGACACGTTTGGATGGAAGGAGCTTCAGAAGGTTGATAAAGAGCTTATTGCGGATACCACGGCAGAAGAATCTGAGAAGGTTGCTAGATGGAAACGTAATGATAATTTCTTTTTTGAGAAAGATGCGCAAGGGTATGGTGTCATTACAATTGATACGAGAAAACCTTTTTGGCTATCGTTGCAGGGGCTTGGGGCGATTGATCCTTATAAATGGAACCAATTCCTTTCTCAACCAAAGCATCCTAAAGGAGTAATTGTGATGGTTCAAACAACCGGGAAAACCTGA", "aa": "MKKWIKKRMWWVLVILLLVPIFFKTGIILYNRIDASSENWKYFSCKLTGKEQTMINCSDKNKDFIISGDQVSFAGRGSWNSVYIPFSSDRITLRIDPETRAGYDFWVLYDTFGWKELQKVDKELIADTTAEESEKVARWKRNDNFFFEKDAQGYGVITIDTRKPFWLSLQGLGAIDPYKWNQFLSQPKHPKGVIVMVQTTGKT", "aa_hexdigest": "b8cf5ed7edd2ff4524a90fe386edac44", "start_type": "ATG", "rbs_motif": "TTCTT", "psc": { "uniref90_id": "UniRef90_A0A328EGF9", "query_cov": 1.0, "subject_cov": 0.9398148148148148, "identity": 1.0, "score": 424.0, "evalue": 3.52e-150, "valid": true, "uniref50_id": "UniRef50_A0A328EGF9" }, "pscc": { "uniref50_id": "UniRef50_A0A328EGF9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EGF9" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 23746.098500000004, "isoelectric_point": 9.299773979187012 }, "id": "NKOCPLAKPK_638", "locus": "GCA003260355_00602" }, { "type": "cds", "sequence": "contig_1", "start": 321721, "stop": 321903, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAACGAAAAGAATCTCTCAATTTACAATTCTATCTTCGTTATTCGGAATGTTTTATTCCCTTTTTGGAGCACTGTTATTTATTTTAACGCTCAATGCTCACAATAAATTAATCATGAACAACACAATTAGTTCTCTCTTTGGGGAATCTGCTAATAGTGTTGGAATGTTTATCTCTTGA", "aa": "MQTKRISQFTILSSLFGMFYSLFGALLFILTLNAHNKLIMNNTISSLFGESANSVGMFIS", "aa_hexdigest": "688c1234eb6eb189d949180f091722bc", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6667.789299999999, "isoelectric_point": 9.697157096862792 }, "id": "NKOCPLAKPK_639", "locus": "GCA003260355_00603" }, { "type": "cds", "sequence": "contig_1", "start": 322244, "stop": 322438, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTCGTCCTCGCGTTATATAACTTTGTGGATTCCATCTTTATCGGAAAATATGTCGGAATGGAAGGGCTTGCTGCGACAAGTATCGTATTCCCGATTCAGATGATTATTGGAGCGTTTGCGATGGCGTTCGGTGTGGGAGCGGCGTCTATTATTTCTAGGAGTATCGGTGCAAAAGCATATGAAAAAGTTTGA", "aa": "MLVLALYNFVDSIFIGKYVGMEGLAATSIVFPIQMIIGAFAMAFGVGAASIISRSIGAKAYEKV", "aa_hexdigest": "458c8c0d6ab9e3263dd0548ed2215ee7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6734.016200000001, "isoelectric_point": 8.098276710510255 }, "id": "NKOCPLAKPK_640", "locus": "GCA003260355_00604" }, { "type": "cds", "sequence": "contig_1", "start": 322511, "stop": 323509, "strand": "+", "frame": 2, "gene": "mepA", "product": "Multidrug export protein MepA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EJQ9", "UniRef:UniRef90_A0A328EJQ9" ], "nt": "GTGCCGCAGCTCTTGGATCTTTTTGGAGCAACGCCTGAGAGTATGGAGTTTGCGAGAGCATATATGGTGCCTCTCTTATTCGGAGCGGTCTTTTTGGGATTTGAGATGTCCAACAACAATATTATTAGGTCGGTCGGGCATGCGACGATGAGTATGAAGATCATGCTTACGAGTGCGATCACCAATTTGATTCTGGATTTTGTCTTTATGAAGATATTTGGCTGGGGAATGGCTGGGGCTGCTTGGGCGACGGTGATTTCCTGGATCGTTTCTTGCGGGATGATGCTGTGGTATTATTTGAGTCCAAAGAATGTGATCCCGATCGCGATCAAGCATCTTCGTATTCATTTGCAGAGTGCAAAAGAAATAATTCTGATTGGACTGCCGAGTTTTTTTAGGCAGATTGTGGCGAGTTTAACGACGGCGTTGATCAATAATTATCTCAGGAAGTATGGAGGGACGGAAGCGATTGCGATTTATGGGATTGTATACAAAGTCTTGCAGCTGTATATGATGCCGATGTTTGGAATCGTGCAGGGAATGCAACCGATTATCGGCTATAATTATGGAGCGAAAAGTCTTGATCGTGTGAAAAAAGTCGTTCTGCTTGGAATTCAGATTTTGACGCTTTTTACGACAGCGATTTCTTTGATTTATCGGGTGTTCCCGACTTTTTTGGTGTCGTTTTTTTCTGAGGATCCACAGCTGATTGCCAATACGGGAAAGGTGCTTCAGATGGTCGTAGCGTGCTTTGCAGTAGTAGGATTTCAGGTGGTTTCCTCTTCTATGTATCAGGCTTTGGGGTTTGTGAAAAAGGCGTTTCTTTTGACCTTACTCAGGCAACTTATCGTTTTTATCCCGGTGCTTTTCTTGATGCTTTGGCTGCGAGAGGATAAACTCCAAGCGGTTTGGCTCAGTTTCCCAATTACTGATGCGATCGGAGTGGTGTTTACGGTGCTGATTTTTGTGCCTGATTTTAAGAAGATGAAGTCGGCGTAG", "aa": "MPQLLDLFGATPESMEFARAYMVPLLFGAVFLGFEMSNNNIIRSVGHATMSMKIMLTSAITNLILDFVFMKIFGWGMAGAAWATVISWIVSCGMMLWYYLSPKNVIPIAIKHLRIHLQSAKEIILIGLPSFFRQIVASLTTALINNYLRKYGGTEAIAIYGIVYKVLQLYMMPMFGIVQGMQPIIGYNYGAKSLDRVKKVVLLGIQILTLFTTAISLIYRVFPTFLVSFFSEDPQLIANTGKVLQMVVACFAVVGFQVVSSSMYQALGFVKKAFLLTLLRQLIVFIPVLFLMLWLREDKLQAVWLSFPITDAIGVVFTVLIFVPDFKKMKSA", "aa_hexdigest": "7dfc16119cf7ea375588e675e8630eb7", "start_type": "GTG", "rbs_motif": null, "genes": [ "mepA" ], "truncated": "5-prime", "pseudogene": { "start": 322211, "stop": 323509, "observations": { "mutation": [ 322436, 322499 ], "stop": [ 322436, 322499 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EJQ9", "query_cov": 1.0, "subject_cov": 0.7410714285714286, "identity": 0.997, "score": 624.0, "evalue": 1.79e-223, "gene_start": 1, "gene_end": 332, "reference_start": 117, "reference_end": 448, "reference_sequence": "MEAETKQLGTQTIGKLLLKQSAPAVIGMLVLALYNFVDSIFIGKYVGMEGLAATSIVFPIQMIIGAFAMAFGVGAASIISRSIGAKAYEKVGQVFGTLQLMTFGFLVILSVVGLCFVPQLLDLFGATPESMEFARAYMVPLLFGAVFLGFEMSNNNIIRSVGHATMSMKIMLTSAITNLILDFVFMKIFGWGMAGAAWATVISWIVSCGMMLWYYLSPKNVIPIAIKHLRIHLQSAKEIILIGLPSFFRQIVASLTTALINNYLRKYGGTEAIAIYGIVYKVLQLYMMPMFGIVQGMQPIIGYNYGAKSLDRVKKVVLLGIQILTLFTTAISLIYRVFPTFLVSFFSEDPQLIANTGKVLQMVVACFAVVGFQVVSSSMYQALGFVKKAFLLTLLRQLIVFIPVLFLMLWLREDKLQAVWLSFPITDAIGVVFTVLIFVPDFKKMKSA" }, "paralog": false, "identity": 0.9953703703703703, "score": 828.0, "evalue": 3.23e-306, "description": "Internal stop codon at 322436, 322499. Nonsense mutation around 322436, 322499.", "psc": { "uniref90_id": "UniRef90_A0A328EJQ9", "product": "Multidrug export protein MepA", "uniref50_id": "UniRef50_A0A328EJQ9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EJQ9", "UniRef:UniRef50_A0A328EJQ9" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EJQ9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EJQ9" ], "product": "Multidrug export protein MepA" } }, "id": "NKOCPLAKPK_641", "locus": "GCA003260355_00605" }, { "type": "cds", "sequence": "contig_1", "start": 323799, "stop": 324308, "strand": "+", "frame": 3, "gene": null, "product": "Thymidylate kinase", "db_xrefs": [ "EC:2.7.4.9", "KEGG:K00943", "SO:0001217", "UniRef:UniRef50_A0A136KKR1", "UniRef:UniRef90_A0A328EG91" ], "nt": "TTGAGCGGGAGCTTTGGGACGGCGGCTGAAGTCTCTCCCTATCAGGCTTCGGTGCTGTATGCGGTAGATAGGTTTTCTGCCTCTTTTCAGATCAGGAAATGGCTTGAAGAAGGGAAGATTGTGATCTCTGATCGTTATGTATCTGCGAATATGGGGCATCAGGCAGGAAAGATTGATGATCTCGCAGAAAGGGACCGCTTTTTGGAGTGGTTGGAAGCGTTTGAATACGGGCTTTTGGGGATTCCCCGTCCTGATATTCAGATTTTTCTCTATTTAGATCCAGAAATTAGTAGAAATCTGGCTCTGAAAGTTGAAAAGCCTAATATGGACAAATCAAAAGATATTCATGAAAACGATGCTGAACATATGAGAAAGGCAAGTGAGGCTTTTCGTTATGTGGCAGAGAAGTATGGCTGGACTCAAATTGCTTGTGCTGAGCAGGGTGAGATGAAAACTAGAGAGCAGATTTCCAGTGAACTTTATCAGAAGATTGCTTCGATGCTTGCGTAA", "aa": "MSGSFGTAAEVSPYQASVLYAVDRFSASFQIRKWLEEGKIVISDRYVSANMGHQAGKIDDLAERDRFLEWLEAFEYGLLGIPRPDIQIFLYLDPEISRNLALKVEKPNMDKSKDIHENDAEHMRKASEAFRYVAEKYGWTQIACAEQGEMKTREQISSELYQKIASMLA", "aa_hexdigest": "a96f937ee28cce6ec6f1847ccab8af4d", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 323634, "stop": 324308, "observations": { "mutation": [ 323682 ], "stop": [ 323682 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EG91", "query_cov": 1.0, "subject_cov": 0.7544642857142857, "identity": 0.9940000000000001, "score": 332.0, "evalue": 1.57e-114, "gene_start": 1, "gene_end": 169, "reference_start": 56, "reference_end": 224, "reference_sequence": "MQKTGKLIVIDGTDGSGKKTQTELLVQRLTSEGYEVEKIDFPQYGKKSAGPTEEYLSGSFGTAAEVSPYQASVLYAVDRFSASFQIRKWLEEGKIVISDRYVSANMGHQAGKIDDLAERDRFLEWLEAFEYGLLGIPRPDIQIFLYLDPEISRNLALKVEKPNMDKSKDIHENDAEHMRKASEAFRYVAEKYGWTQIACAEQGEMKTREQISSELYQKIASMLA" }, "paralog": false, "identity": 0.9955357142857143, "score": 443.0, "evalue": 1.79e-160, "description": "Internal stop codon at 323682. Nonsense mutation around 323682.", "psc": { "uniref90_id": "UniRef90_A0A328EG91", "product": "Thymidylate kinase", "ec_ids": [ "2.7.4.9" ], "uniref50_id": "UniRef50_A0A136KKR1", "kegg_orthology_id": "K00943", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EG91", "EC:2.7.4.9", "UniRef:UniRef50_A0A136KKR1", "KEGG:K00943" ] }, "pscc": { "uniref50_id": "UniRef50_A0A136KKR1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A136KKR1" ], "product": "Thymidylate kinase" } }, "id": "NKOCPLAKPK_642", "locus": "GCA003260355_00606" }, { "type": "cds", "sequence": "contig_1", "start": 324388, "stop": 324597, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBF432C", "UniRef:UniRef100_A0A328EFV7", "UniRef:UniRef50_A0A328EFV7", "UniRef:UniRef90_A0A328EFV7" ], "nt": "ATGTCTCTAGAACAAAATCTGCACAAAAAGATGATGAAAATGATTCTTGGAACTGTTGTTCATATTGAAGATACTGAGCAATCAGAGGCGGTTTTGTCTGCTATGGAAGATACTGCAAAACCTAAAGCTCGTGGTATAAAGCAGTTTTTCTTGTCTGGAATCAACGAACTGAAGAGGGATATTAAGAAAGGAAAGAAGAAATCAGCATAA", "aa": "MSLEQNLHKKMMKMILGTVVHIEDTEQSEAVLSAMEDTAKPKARGIKQFFLSGINELKRDIKKGKKKSA", "aa_hexdigest": "96a9833b5724161527648dda00bc592f", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000DBF432C", "uniref100_id": "UniRef100_A0A328EFV7", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBF432C", "UniRef:UniRef100_A0A328EFV7" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EFV7", "uniref90_id": "UniRef90_A0A328EFV7" }, "psc": { "uniref90_id": "UniRef90_A0A328EFV7", "uniref50_id": "UniRef50_A0A328EFV7" }, "pscc": { "uniref50_id": "UniRef50_A0A328EFV7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EFV7" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7787.134199999995, "isoelectric_point": 9.697737312316896 }, "id": "NKOCPLAKPK_643", "locus": "GCA003260355_00607" }, { "type": "cds", "sequence": "contig_1", "start": 324726, "stop": 324902, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCGCCTCATAGTGTCCAACCCAACGCTAAAGTCAGCTTTTCTTATGTTGCGGTAGATCCAGAGACGAAGGAGACTGTTGCCTCCGGGACGAAGAAAGATATGCTGCTTGACTTTACAGGGACTGCTCTTTATCAGCTTTTGGAGGGAGCAAAGAAGGATGAAGTCAGAACTTGA", "aa": "MSPHSVQPNAKVSFSYVAVDPETKETVASGTKKDMLLDFTGTALYQLLEGAKKDEVRT", "aa_hexdigest": "2623a5309dbae9d345d9de8eea1fa73d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6331.0996, "isoelectric_point": 5.636347770690917 }, "id": "NKOCPLAKPK_644", "locus": "GCA003260355_00608" }, { "type": "cds", "sequence": "contig_1", "start": 324981, "stop": 325142, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCTAGTGAGGTAGGAACTCGCGTGACTATGGGAGATGCGTCGTTTTTGACAACGGCTGTTGTGACTGAAGATGGGGTAGAGCAGGTTGTTTTGGATGGGAATCCTGTGGAGACTATAGTGCCTTTGAATTGGTCATTTACGATCACTGAGATTAAGTAG", "aa": "MPSEVGTRVTMGDASFLTTAVVTEDGVEQVVLDGNPVETIVPLNWSFTITEIK", "aa_hexdigest": "168da86679ce4df5eba6c30ec0a71e38", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5695.383699999998, "isoelectric_point": 4.0500284194946286 }, "id": "NKOCPLAKPK_645", "locus": "GCA003260355_00609" }, { "type": "cds", "sequence": "contig_1", "start": 325116, "stop": 325448, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTATGAAAAATTCAATAGAGATTATCATCTCTGCAACCGACAGAGCTTCTTCCGCTTTGCAAGAATCTTCTCGTTCTCTCAAAGATTGGGCAACAAAAAATGAAATTCCTCTACAGAGACTAAAAATAGTCGGACAAGAGACCTTTTCAATACTCTCTAATAAAGTTCTGGAGTTCACAGATAAAGCCAGCAAGACCAAAGAAGCAGAAGATGCAATGAGGAGTAAACTCAATAAAATCCCGGACAAAAAGATCCTCATTCAAAAAAAGGCTCCCCTACAAAAGGGGACCCAGACAAAAAAACTACTTAATCTCAGTGATCGTAAATGA", "aa": "MAMKNSIEIIISATDRASSALQESSRSLKDWATKNEIPLQRLKIVGQETFSILSNKVLEFTDKASKTKEAEDAMRSKLNKIPDKKILIQKKAPLQKGTQTKKLLNLSDRK", "aa_hexdigest": "c3ba2fdc2329f9bd70b6fdd6468b1a54", "start_type": "ATG", "rbs_motif": "TTTA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12399.334699999976, "isoelectric_point": 9.995387840270997 }, "id": "NKOCPLAKPK_646", "locus": "GCA003260355_00610" }, { "type": "crispr", "strand": "?", "repeats": [ { "strand": "?", "start": 325753, "stop": 325790 }, { "strand": "?", "start": 325826, "stop": 325863 }, { "strand": "?", "start": 325895, "stop": 325932 } ], "spacers": [ { "strand": "?", "start": 325791, "stop": 325825, "sequence": "ACAGAGCTGCATAATGATATTGCTCTCCACCTTGT" }, { "strand": "?", "start": 325864, "stop": 325894, "sequence": "ATCCGAATATATGGTAGCATTACAATACTGA" }, { "strand": "?", "start": 325933, "stop": 325942, "sequence": "GCTAAATCTT" } ], "sequence": "contig_1", "start": 325753, "stop": 325942, "product": "CRISPR array with 3 repeats of length 38, consensus sequence CATTTAAAGAAAGCTCCAAACAAGGAGCATCGAAACTC and spacer length 33", "spacer_length": 33, "repeat_length": 38, "repeat_consensus": "CATTTAAAGAAAGCTCCAAACAAGGAGCATCGAAACTC", "db_xrefs": [ "SO:0001459" ], "nt": "CATTTAAAGAAAGCTCCAAACGAGGAGCATCGAAATTCACAGAGCTGCATAATGATATTGCTCTCCACCTTGTCATTTAAAAAAAGCTCCAAACAAGGAGCATCGAAACAAATCCGAATATATGGTAGCATTACAATACTGAGCTTTAGAGAAAGCTCCAAACAAGGAGCATCGAAACTCGCTAAATCTT", "id": "NKOCPLAKPK_45" }, { "type": "cds", "sequence": "contig_1", "start": 326236, "stop": 326910, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCATCTTTTTAAGAGGCTTTTGCTGACGAAGTTTCCCAATTCAACAATTCATAATGCTGTCAATCCTGCAAGTATGTTTATTGGCTTCATTTCTGCCAAGGAATTTTTTTCGATTATTAATAGGAAGATTATAGAAAATCATAAGCTGAATCTCTTTGATGTGAACTTGGATCCCATAGAATTTAAAATGGGATTTGGAGAGAATCCAAATTCTGATAAACAGAAAGATTTAGAAATTTATTTTGCTTATGCGGTAGCTGCAAATCTCAATGGAACGCTCTTTTTTTCTCCTGCGAATCTAGCAAATGGAGTTTCTTTGCTTGCTACGCTGTATTTTAAGCAAGCGATGACGAGTTTTCCTGAATATTACTTTGTTCAGCTGATTTATACGGTTTTTTTGCTGAGTTTTACCTTTGTATCGAGGGTTAAGGTTTTTCCATCAGAACATGATCAGGAGAATTATAATTGGTTTGTTGAGGTTTTCTTTGATTTTTACAAGATTACTTTTGAACAGTTACAAACTAAGATTTCTGATTCAGATTTTTTTGCTGTTAAAAAGGCAGTTTTACAAGAAACGGCATTCTGCTTTCTCCTTTTCCATTTTTATAAAAAGCTTAATAGTCTGCTTGCTGAGAAATCTTCGGATACTGATTATTTAGATTGGCTTCTTTGA", "aa": "MHLFKRLLLTKFPNSTIHNAVNPASMFIGFISAKEFFSIINRKIIENHKLNLFDVNLDPIEFKMGFGENPNSDKQKDLEIYFAYAVAANLNGTLFFSPANLANGVSLLATLYFKQAMTSFPEYYFVQLIYTVFLLSFTFVSRVKVFPSEHDQENYNWFVEVFFDFYKITFEQLQTKISDSDFFAVKKAVLQETAFCFLLFHFYKKLNSLLAEKSSDTDYLDWLL", "aa_hexdigest": "3c95e13c2d081b8e4da545216580035a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 26174.826600000015, "isoelectric_point": 5.952315711975097 }, "id": "NKOCPLAKPK_647", "locus": "GCA003260355_00611" }, { "type": "cds", "sequence": "contig_1", "start": 326929, "stop": 327204, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATCAAGGCTTTTAAGGAGAACTATGCGACAACAAAATATCTTCCTCATTCTTCTGCACTGGAGCAGAGTATCCTCAATATGGTGCGGCCTGCGGATATTTTGATTAAATATCTTTTTGGTGATGCGAATCCGATTATTGCCGTGGAGACGATCGTTGCAAGGATTTTTGATAAGACAGAGCTTGATCCATTGGTGCAATCCTTCCTTACTTCTGATGAGCAACTTCCTCAGCTTTTTGAGTATCTTTTGGAGTATAAAAAATATAAATACTGA", "aa": "MIKAFKENYATTKYLPHSSALEQSILNMVRPADILIKYLFGDANPIIAVETIVARIFDKTELDPLVQSFLTSDEQLPQLFEYLLEYKKYKY", "aa_hexdigest": "c6e9025268293647bfb2a02537e9cc4d", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10608.191999999994, "isoelectric_point": 5.2846281051635735 }, "id": "NKOCPLAKPK_648", "locus": "GCA003260355_00612" }, { "type": "cds", "sequence": "contig_1", "start": 327286, "stop": 327996, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTCTCCGCGATTGACAATGGGGACGATATTTCCAATTTTGATGTGCCTGAGAGGATTAAAAGGGAATCTAAAGTGACGGAGAGGCTTCTTAATTTCTACGTTACTCTGCTCGGTGGCTTTACGACTGCTAGAGGAGACAGCTTTTATTTAAGGCTTTTGAAGCCGGAAATTTTGGATTTTTTTACAAAAAATTCTCTCAATAGTTTGCTGGGGTCGGAGGTTCAGCTTGAGTATTTCGGTGGTGTTTTGTACCAATATGCCAAAAATCTGTACTACTACAGCTATATTAATGAGAATATCAGAGCTGGGAAAAACAAGTTTTCTATGCCACTTAAAGGCGATAGTTCCAAGGTGACGACCAACTTTTCTGTGATTAAACTTTATACTGAGGGGATGATTGCTTCTTTTTTTCAAGATTTAAATCCCAAAGATACCAAACTTACTATTAAAAATACTCAGATTTTAGAACTTTTTAAAACACAGTTTGGAGAACAGGTTTCGGAGATGGTTAAGTTAGGGGCTGATAAATTTTTAGAGGCTTTTTATGCGCCGATTCTTTCACAGATCAAAGATTCAAAGGCTTTTGTGCGTGTTTTATCCTCATCTTTGCAGGAAAATGATCTTGTCAATCTCAAAGATGCACTTTATAAACTAGATTTTTGGATCAGTTTTTCGTTTTTTAAAAAGTTAGAAGCCTTGAAGCTCTGA", "aa": "MLSAIDNGDDISNFDVPERIKRESKVTERLLNFYVTLLGGFTTARGDSFYLRLLKPEILDFFTKNSLNSLLGSEVQLEYFGGVLYQYAKNLYYYSYINENIRAGKNKFSMPLKGDSSKVTTNFSVIKLYTEGMIASFFQDLNPKDTKLTIKNTQILELFKTQFGEQVSEMVKLGADKFLEAFYAPILSQIKDSKAFVRVLSSSLQENDLVNLKDALYKLDFWISFSFFKKLEALKL", "aa_hexdigest": "3953b5bf8f673bd5eccd3930c63bc3c4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 27101.85620000002, "isoelectric_point": 8.482443809509277 }, "id": "NKOCPLAKPK_649", "locus": "GCA003260355_00613" }, { "type": "cds", "sequence": "contig_1", "start": 328018, "stop": 328419, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTTGCGCTTTTTGGAAGTATCAGGGAGACATTCTTTGGATTAGCATTGCTATTTGTTTATCTTGAGCAGAAAGAAAAGGCTGCTTCTCGTGAAAAAAATGAGATTCTTTTGATGGTCTATGTGCGCGATATTTTGGGGATAAAAATTAAAAAAGCTGATCAGGTTTTTAAGGTAATGGTAGAGACGATAGAAGAGCTAAAGCCAATTTTGAAGCTTTGGATTTCTCTGGATGATAATAAAGGTTTCTTTGATCTTATTGCAAAAAATTGGGATTCTTTTTGTGCAGAAAAAACTGAAGATCAACTTCTCGCTTCTTTTTCTGGCGAAGATTTGGTGTGGTTTAGAGGCTTATTAAAGAATATTGCCTATTATAATAAGAGATTTGTGATGCCAAGGTAG", "aa": "MLALFGSIRETFFGLALLFVYLEQKEKAASREKNEILLMVYVRDILGIKIKKADQVFKVMVETIEELKPILKLWISLDDNKGFFDLIAKNWDSFCAEKTEDQLLASFSGEDLVWFRGLLKNIAYYNKRFVMPR", "aa_hexdigest": "da87389e69d507980297d4a2c4385855", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15580.189899999987, "isoelectric_point": 7.755930519104004 }, "id": "NKOCPLAKPK_650", "locus": "GCA003260355_00614" }, { "type": "cds", "sequence": "contig_1", "start": 329270, "stop": 329404, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCATGTAGAACATGGTTCTGGACTAGTGAAGGGATTGGTTCGGTGGAAATCTTGGATTTCAAATGCCTATGATTATACCCTCGGAAAACTGTGTTTCAATAGTGCGGATGAGATCGTAGCTATTAGTAAATGA", "aa": "MHVEHGSGLVKGLVRWKSWISNAYDYTLGKLCFNSADEIVAISK", "aa_hexdigest": "1fe9c6c4fc9fb8596f3e65b9cf0c6eae", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4924.6122, "isoelectric_point": 7.928402519226075 }, "id": "NKOCPLAKPK_651", "locus": "GCA003260355_00615" }, { "type": "cds", "sequence": "contig_1", "start": 329447, "stop": 329887, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13692.12" ], "nt": "GTGATTTATAGGGGATTGGATTTTCCAGAGATGAGGAGAAAAGAGAAAAAGACTGATCAGGTTTCCCTTGGTTTTGTTGGAAGACTGGTAAAATTGAAGGGAGTGGACCTTTTGATTTCTGCCTTTGCTGACTTAGTCGTTGAAAATCCTCATTTGACCTTGCAAATTGTTGGAGATGGCGAAGAGAGGGGGAGTCTTGAAAAGCGGGTGAAGAACTTAGGATTAGAAGGGAAAGTGCAGTTTTTGGGATTTCAGGATAAGGAGGAAATTCAGCAGAGACTTCTCCCTTCCTGGGATATTTTTGTGAGTCCGAGTCTCCAAGAAGGTTTACCGACGACGGTGATTGAAGCTTTGTTTTCTCAGTGTATTACTGTTGCTACTGATGTCGGTGGAACGAGGGAAATCGCTGATGGAGAGGATTTTATTGTCATTGAGCCTTGA", "aa": "MIYRGLDFPEMRRKEKKTDQVSLGFVGRLVKLKGVDLLISAFADLVVENPHLTLQIVGDGEERGSLEKRVKNLGLEGKVQFLGFQDKEEIQQRLLPSWDIFVSPSLQEGLPTTVIEALFSQCITVATDVGGTREIADGEDFIVIEP", "aa_hexdigest": "2059c489153f1125dfa039eb5cb19645", "start_type": "GTG", "rbs_motif": "TTCTA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13692.12", "name": "Glyco_trans_1_4", "length": 128, "aa_cov": 0.8493150684931506, "hmm_cov": 0.9609375, "evalue": 1.7936443512879637e-29, "score": 99.29043579101562, "start": 22, "stop": 145 } ], "seq_stats": { "molecular_weight": 16272.522199999989, "isoelectric_point": 4.782057762145995 }, "id": "NKOCPLAKPK_652", "locus": "GCA003260355_00616" }, { "type": "cds", "sequence": "contig_1", "start": 330031, "stop": 330204, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAGTAAAATCTGAGACGGATAGAAAATACTATTTCTTGATCAATTCTTTGGAGATAGGAGGTGCTGAGAGGGTCGTTATCAACCAGGCAAATGAGTTAATCAAAAAAGGATTTCAAGTTTATGTTTTTACTTTGAAGTCTGCTTGTTTATACGATTTACCAAAATGA", "aa": "MKKVKSETDRKYYFLINSLEIGGAERVVINQANELIKKGFQVYVFTLKSACLYDLPK", "aa_hexdigest": "eb411edd521adb818fdd4edc48bd0225", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6601.688700000001, "isoelectric_point": 9.37417049407959 }, "id": "NKOCPLAKPK_653", "locus": "GCA003260355_00617" }, { "type": "cds", "sequence": "contig_1", "start": 330328, "stop": 330738, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13439.12" ], "nt": "TTGGAAATCTCTAATTTTATTCATATTTTAGCTAGGCGTGAAGCGAAGATTTCTTTTAGAACACATATAAATTTTTTTACAGGTTTTTTTGGATGGCTTCAAAAACTCTTTATAAAAATTCTTTATCCGAGGGCAGCTAAAATTATTGTGAACTCTAGAGAAAATAGACACGATCTTGCTGCTTATTTAGGCATTTCAGAGCAAAAAATTGAAGTTGTCTATAATAGTATTGATGAGGAGAAAATAATGCTGCTTTCTAGAGAGGCTCTTGAGGATGAACTTCAGAAAAAAATTAGAAATAAGCGTGTTTACATTACCGTCGGTAGACTTATCAAAGAGAAACACCATGAAATAATTATGGATGCGTTATCTTGTTTGAAGAATAAAAATTGGATATGGCTTATTGTCTGA", "aa": "MEISNFIHILARREAKISFRTHINFFTGFFGWLQKLFIKILYPRAAKIIVNSRENRHDLAAYLGISEQKIEVVYNSIDEEKIMLLSREALEDELQKKIRNKRVYITVGRLIKEKHHEIIMDALSCLKNKNWIWLIV", "aa_hexdigest": "33c618c622876e9826b532cb3b62f6ef", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13439.12", "name": "Glyco_transf_4", "length": 71, "aa_cov": 0.47058823529411764, "hmm_cov": 1.0, "evalue": 1.175123089195693e-10, "score": 38.15693664550781, "start": 17, "stop": 80 } ], "seq_stats": { "molecular_weight": 16225.945500000002, "isoelectric_point": 9.637781715393068 }, "id": "NKOCPLAKPK_654", "locus": "GCA003260355_00618" }, { "type": "cds", "sequence": "contig_1", "start": 330861, "stop": 330965, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACCTCTCTCGCTCCACTCTTAAAATCAGTCGTAATAATCGGAATCCCTAAAAATCTTGCCTCCAATAAGACATTTGGAAATCCTTCTACAGAGGAAGCATAA", "aa": "MTSLAPLLKSVVIIGIPKNLASNKTFGNPSTEEA", "aa_hexdigest": "f602f15470306bead3268bb5bc6b87f6", "start_type": "ATG", "rbs_motif": "TTGTA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3542.105800000001, "isoelectric_point": 8.254934883117677 }, "id": "NKOCPLAKPK_655", "locus": "GCA003260355_00619" }, { "type": "cds", "sequence": "contig_1", "start": 331134, "stop": 331238, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATTACCTTATTATGCAAAAAGATATATAGAAGGAAATAAAAGTGCTTCTAAATCTTTAGGTTGGAAAGATGAGAAGGTTGATCTTTTGCTGAAAAAATGA", "aa": "MKLPYYAKRYIEGNKSASKSLGWKDEKVDLLLKK", "aa_hexdigest": "a150448929c688333f6122447f0b6dad", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4001.6924, "isoelectric_point": 9.756274604797362 }, "id": "NKOCPLAKPK_656", "locus": "GCA003260355_00620" }, { "type": "cds", "sequence": "contig_1", "start": 331491, "stop": 331754, "strand": "+", "frame": 3, "gene": null, "product": "Class I SAM-dependent methyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MX25", "UniRef:UniRef90_A0A660MX25" ], "nt": "ATGTCGAGAGTTTTAAAAAAAGGAGGAAAATTGATGCTTTTCACTCCTACACCTTATACTTGGTATTTCTGGGATGATCCTACTCATCAGAGGCCTTCGACTCATGGTTCGCTAGAACATTTGGCAAAGGATGCTGGTTTGAGAGTGATTGAATCTAAATATTCTAATACGAGATTTTTCCCTCAAAGTGCTCAGAAATGGCTTAGATTGCCTCCATTTAGGTCTTTCCTCTGGGAAGTATATTTAGTAGCTGAGAAGTCTTAA", "aa": "MSRVLKKGGKLMLFTPTPYTWYFWDDPTHQRPSTHGSLEHLAKDAGLRVIESKYSNTRFFPQSAQKWLRLPPFRSFLWEVYLVAEKS", "aa_hexdigest": "8d73287cf7ae65c72eddf37755b1ccec", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 331191, "stop": 331754, "observations": { "mutation": [ 331236, 331257, 331359, 331377, 331485 ], "stop": [ 331236, 331257, 331359, 331377, 331485 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A660MX25", "query_cov": 1.0, "subject_cov": 0.4223300970873786, "identity": 1.0, "score": 190.0, "evalue": 4.41e-60, "gene_start": 1, "gene_end": 87, "reference_start": 120, "reference_end": 206, "reference_sequence": "MKLPYYAKRYIEGNKSASKSLGWKDEKVDLLLKKGDVVLDIGCAVGGFYDKRKHKKIDFHGVDYNPDLVKFCNEKGLQVKECDISKGKLDYPDETFDFIYCSHVLEHILSNEQIALFGEMSRVLKKGGKLMLFTPTPYTWYFWDDPTHQRPSTHGSLEHLAKDAGLRVIESKYSNTRFFPQSAQKWLRLPPFRSFLWEVYLVAEKS" }, "paralog": false, "identity": 0.9732620320855615, "score": 377.0, "evalue": 3.12e-136, "description": "Internal stop codon at 331236, 331257, 331359, 331377, 331485. Nonsense mutation around 331236, 331257, 331359, 331377, 331485.", "psc": { "uniref90_id": "UniRef90_A0A660MX25", "product": "Class I SAM-dependent methyltransferase", "uniref50_id": "UniRef50_A0A660MX25", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A660MX25", "UniRef:UniRef50_A0A660MX25" ] }, "pscc": { "uniref50_id": "UniRef50_A0A660MX25", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MX25" ], "product": "Class I SAM-dependent methyltransferase" } }, "id": "NKOCPLAKPK_657", "locus": "GCA003260355_00621" }, { "type": "cds", "sequence": "contig_1", "start": 331756, "stop": 332100, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAGATTAAGCATAATGTTTTGGGGAAGGGAGACAAGAAGATACTCTTTGATATTGAGATGCTACTCTATTATAAACAAGAGTTTGATGAGTTGGTGAAGAGTGGAGAATATACCTTCTATTTTTTGGAAAGGAAGCAGTTTACTTCTCTATTTTCTCACGATCTTTTGAATAGAGTTTCGTTTTCTGGTTTGGATAAAACCTATAAGAAAAATTCTGATTTAAAAGATTATTATCAATCATTATTATCCTTTTGTAGAGAAGAGAAAGTAAAGGCTTGTGTTTTTACAAGTACAGGACAGTATTGGCATCCAGATTTCCTAAATTTATTAAAAAGGGAATGA", "aa": "MQIKHNVLGKGDKKILFDIEMLLYYKQEFDELVKSGEYTFYFLERKQFTSLFSHDLLNRVSFSGLDKTYKKNSDLKDYYQSLLSFCREEKVKACVFTSTGQYWHPDFLNLLKRE", "aa_hexdigest": "2dbf07937ba133d104198897635d30be", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13719.581299999982, "isoelectric_point": 8.45078983306885 }, "id": "NKOCPLAKPK_658", "locus": "GCA003260355_00622" }, { "type": "cds", "sequence": "contig_1", "start": 332659, "stop": 332853, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13524.12" ], "nt": "ATGTCTAGGGTATTTGAAGATGGGAAAGAGATTGTTTGTTATGAAAGTATTGATGAGGCTATTGAGAAGATTGAGTATTACTTGAAAAATGAGAAGGAAAGAATAGAAATAGCAAAGGCAGCTTATCTTAGGTCCAAGAATGAATATGCATACTTACATGTAATGAAAAAAACGCTTGATTATATTTGTGGATAG", "aa": "MSRVFEDGKEIVCYESIDEAIEKIEYYLKNEKERIEIAKAAYLRSKNEYAYLHVMKKTLDYICG", "aa_hexdigest": "110dd2f253e5fad9ea0af60062925490", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13524.12", "name": "Glyco_trans_1_2", "length": 59, "aa_cov": 0.921875, "hmm_cov": 1.0, "evalue": 1.1551162879340067e-14, "score": 50.990230560302734, "start": 2, "stop": 60 } ], "seq_stats": { "molecular_weight": 7645.715999999999, "isoelectric_point": 5.371478080749512 }, "id": "NKOCPLAKPK_659", "locus": "GCA003260355_00623" }, { "type": "cds", "sequence": "contig_1", "start": 333463, "stop": 333561, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF05050.19" ], "nt": "ATGAAGAAATTTGATGATTTAGACTTAGATTTTGATAAATCAAAAGTACAGTTTATAAAAATCGATGTTGAAGGATATGAATTTGAAGTTTTGAAGTGA", "aa": "MKKFDDLDLDFDKSKVQFIKIDVEGYEFEVLK", "aa_hexdigest": "862257c036f0ed6cf5f4d64b76517c7d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF05050.19", "name": "Methyltransf_21", "length": 28, "aa_cov": 0.875, "hmm_cov": 1.0, "evalue": 3.3224715580680194e-08, "score": 30.278091430664062, "start": 4, "stop": 31 } ], "seq_stats": { "molecular_weight": 3883.4187, "isoelectric_point": 4.518097496032715 }, "id": "NKOCPLAKPK_660", "locus": "GCA003260355_00624" }, { "type": "cds", "sequence": "contig_1", "start": 333562, "stop": 333684, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGGAACTTACTGCAAGAAAACAGAACATTGGAAATTATTCTCGAATTTTCTCCAGAATATTATGAAGAATTTGGAAAAGACTATACTGTTCAGTTTATAGACTTTATTTATGAACTTTGA", "aa": "MRNLLQENRTLEIILEFSPEYYEEFGKDYTVQFIDFIYEL", "aa_hexdigest": "03873cefd3094b955a46af0184b6446d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5010.5804, "isoelectric_point": 4.099933052062987 }, "id": "NKOCPLAKPK_661", "locus": "GCA003260355_00625" }, { "type": "cds", "sequence": "contig_1", "start": 334140, "stop": 334604, "strand": "-", "frame": 2, "gene": null, "product": "Glycosyl transferase family 1 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EI77", "UniRef:UniRef90_A0A328EI77" ], "nt": "ATGGAAGATGCTAACATACCAACAATCATCAGCAGCCTTTTTGGTAATGATTCAAAAATGATCGTATCAATCCATCATAGTATTGCGGCATATGGCAAGGGACTATACCGTTTATGCATTAAATATCTGTATAGATTTGCAGATACTATCACCGTACTTACCAGTTATGAAAGACAACAACTAACACAAAATTTCAAAATTCCAGCACAAAAAGTGCAAATAATTCCTAATGGACTCAATCTAGATGAAATAAAAGAGAAGCAAAAAGCTGCACTCCCAGCAGAATATACTGCTCTCTTTGAAAATAAAAAATTTACATTTATCAGTGTCGGTCGTCTCAATCCTATCAAGAATCAATCCCTCATGATCCAGAGTTTTATCAAACTTAACAAGAAATATCCGGATACTCAGCTTATTATTCTAGGGGAAGGAGAATTAAGACCTCAATTAGAAAAAGAAATCTGA", "aa": "MEDANIPTIISSLFGNDSKMIVSIHHSIAAYGKGLYRLCIKYLYRFADTITVLTSYERQQLTQNFKIPAQKVQIIPNGLNLDEIKEKQKAALPAEYTALFENKKFTFISVGRLNPIKNQSLMIQSFIKLNKKYPDTQLIILGEGELRPQLEKEI", "aa_hexdigest": "56cc30d449fa62cd8ad735f679f6f169", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 333837, "stop": 334883, "observations": { "mutation": [ 333851, 333962, 333980, 334010, 334142, 334733, 334790, 334841, 334844 ], "stop": [ 333851, 333962, 333980, 334010, 334142, 334733, 334790, 334841, 334844 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EI77", "query_cov": 1.0, "subject_cov": 0.4196185286103542, "identity": 1.0, "score": 301.0, "evalue": 1.51e-100, "gene_start": 1, "gene_end": 154, "reference_start": 94, "reference_end": 247, "reference_sequence": "MKNIVIIIPSLQFGGGAEKVASILGTELHKEGYDISYLTFYDSKEKYPFKGTYYTLNEDQTSSFFGKIKKLFTRALKIKKFCKEHKINTVISLMEDANIPTIISSLFGNDSKMIVSIHHSIAAYGKGLYRLCIKYLYRFADTITVLTSYERQQLTQNFKIPAQKVQIIPNGLNLDEIKEKQKAALPAEYTALFENKKFTFISVGRLNPIKNQSLMIQSFIKLNKKYPDTQLIILGEGELRPQLEKEIGDNSNVYLLGNHENIFPFLMKSDCFLLSSLSESFSLAILEAMASGLPIISTKTQGPKDILGENTYGITTPNNDKTAFSSAMELLLNDKECYNSYKEQGRKRYTDFSSDKIVEKRKSALEN" }, "paralog": false, "identity": 0.9741379310344828, "score": 655.0, "evalue": 3.34e-242, "description": "Internal stop codon at 333851, 333962, 333980, 334010, 334142, 334733, 334790, 334841, 334844. Nonsense mutation around 333851, 333962, 333980, 334010, 334142, 334733, 334790, 334841, 334844.", "psc": { "uniref90_id": "UniRef90_A0A328EI77", "product": "Glycosyl transferase family 1 domain-containing protein", "uniref50_id": "UniRef50_A0A328EI77", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EI77", "UniRef:UniRef50_A0A328EI77" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EI77", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EI77" ], "product": "Glycosyl transferase family 1 domain-containing protein" } }, "id": "NKOCPLAKPK_662", "locus": "GCA003260355_00626" }, { "type": "cds", "sequence": "contig_1", "start": 334922, "stop": 335248, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATCAATGCAGCAAAAAAACTGTATCCCTCTAGCAATTATTCAGTAGGATCATTTGATACGATTAAGGGATATGAAATAGACTATCTAATAACAGTTAATTTTATCCATGCTGTCCCACCAGAAGAACTGAAAAGCTACTATGCCACCTTATGCAGAGACAATACCATAAAAACGATTATCGTTGATGAGCTTCACAACAATAACAACTATCGTTTTAATCACAACTTTTTAGAAATTCTTCCATCAGATTATGTGTGTATAAAGAGCTCTCCATACTTTGTTGGGAATAGAAAAATAGTAGTATTTGGTAAAAAAGAAAAATAA", "aa": "MINAAKKLYPSSNYSVGSFDTIKGYEIDYLITVNFIHAVPPEELKSYYATLCRDNTIKTIIVDELHNNNNYRFNHNFLEILPSDYVCIKSSPYFVGNRKIVVFGKKEK", "aa_hexdigest": "b95441d5ee5302475aa36acdefe4504d", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12504.161799999987, "isoelectric_point": 8.489406394958497 }, "id": "NKOCPLAKPK_663", "locus": "GCA003260355_00627" }, { "type": "cds", "sequence": "contig_1", "start": 335555, "stop": 335896, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTCCAGCACATAGTTTTGATGAAAATACATGTAAGGCACTAAAAGAATTAAAATTTGAATATATCACAGATGGGATAGCCCTTTCCCCATTTTCAAGAGAAGGTCTCAAATGGCTGCCTCAGCAACTCCGAAAGCCAATAAAAAAAAGAAGCGGAATCTGGACCATTTGTCTGCATCCCAATAGCTATAGTCCAGCTTTTATGGATAATATAGAAGCATTCTGTAAAGCTGAATCTCAACACTGTATCAATGCTATTGAATCCTTGGATTATAGCTCTCCACGAAGGAAGTCAGTTTTTTTCTATAGATTTTATGCTGAACAAAAACTCTATCGTTGA", "aa": "MAPAHSFDENTCKALKELKFEYITDGIALSPFSREGLKWLPQQLRKPIKKRSGIWTICLHPNSYSPAFMDNIEAFCKAESQHCINAIESLDYSSPRRKSVFFYRFYAEQKLYR", "aa_hexdigest": "97a191ea361e0734fcb55cd5f713ddd2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13245.091499999984, "isoelectric_point": 9.097214317321779 }, "id": "NKOCPLAKPK_664", "locus": "GCA003260355_00628" }, { "type": "cds", "sequence": "contig_1", "start": 336593, "stop": 336757, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03720.21" ], "nt": "ATGCCAGTTCGAGTTGCTCATCAGGCAATAAAGCTCCTTATCCAGGCAAACAACAAAGTACAAAACGCAAATATTCTGATATTAGGCCTAACCTTTAAGGAAAATGTCCCTGACTTTAGAAATAGTAAAATAGCAGAGACCATCAGAGAACTCAAAGAATTCTGA", "aa": "MPVRVAHQAIKLLIQANNKVQNANILILGLTFKENVPDFRNSKIAETIRELKEF", "aa_hexdigest": "9962ee7cd563f7cbc366df78a98457d7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03720.21", "name": "UDPG_MGDP_dh_C", "length": 28, "aa_cov": 0.5185185185185185, "hmm_cov": 1.0, "evalue": 8.527285880938987e-09, "score": 32.22474670410156, "start": 26, "stop": 53 } ], "seq_stats": { "molecular_weight": 6188.208699999999, "isoelectric_point": 9.81745510101318 }, "id": "NKOCPLAKPK_665", "locus": "GCA003260355_00629" }, { "type": "cds", "sequence": "contig_1", "start": 337157, "stop": 337396, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03721.20" ], "nt": "TTGATCGTAACTGTACCAACTCCAGTTGATCATGACAATAACCCTGACTACACACCACTAATCAAAGCATCAACAATGATAGGTAAAGTGCTAGAAAAAGGACAAACTGTTGTATATGAATCGACTGTAGACCCTGGATGCACTGAGGAAATCTGTATCCCTCTTCTAGAAAAAAATAGTGGACTGAAATACATTCAAGATTTTCATGTAGGATACTCTCCAGAAAGAATCAACCCATGA", "aa": "MIVTVPTPVDHDNNPDYTPLIKASTMIGKVLEKGQTVVYESTVDPGCTEEICIPLLEKNSGLKYIQDFHVGYSPERINP", "aa_hexdigest": "79e173f883487aab1afc29df0fd4f10c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03721.20", "name": "UDPG_MGDP_dh_N", "length": 80, "aa_cov": 1.0, "hmm_cov": 0.9875, "evalue": 2.4545709780993525e-17, "score": 59.52571105957031, "start": 1, "stop": 79 } ], "seq_stats": { "molecular_weight": 8760.906999999997, "isoelectric_point": 4.706746101379394 }, "id": "NKOCPLAKPK_666", "locus": "GCA003260355_00630" }, { "type": "cds", "sequence": "contig_1", "start": 337463, "stop": 337615, "strand": "-", "frame": 3, "gene": null, "product": "UDP-glucose 6-dehydrogenase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A482YVT4" ], "nt": "ATGAAAACTATTGCAATCATTGGACTTGGATATGTAGGGCTCCCACTAGCCTACCATCTCGCAAAAAAAGGGAACAATCTCATCGGATTCGACATTTCTGAGCAAAAACTCTCTGAGCTGAAAAATGGATTTGATGCAACAGAAGAAATCTGA", "aa": "MKTIAIIGLGYVGLPLAYHLAKKGNNLIGFDISEQKLSELKNGFDATEEI", "aa_hexdigest": "89fdcb8aea1f104be862f023d96fa28c", "start_type": "ATG", "rbs_motif": "TTTA", "psc": { "uniref90_id": "UniRef90_A0A482YVT4", "query_cov": 0.98, "subject_cov": 0.875, "identity": 0.612, "score": 58.5, "evalue": 1.98e-10, "valid": false, "product": "UDP-glucose 6-dehydrogenase", "uniref50_id": "UniRef50_A0A482YVT4" }, "pscc": { "uniref50_id": "UniRef50_A0A482YVT4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A482YVT4" ], "product": "UDP-glucose 6-dehydrogenase" }, "genes": [], "id": "NKOCPLAKPK_667", "locus": "GCA003260355_00631" }, { "type": "cds", "sequence": "contig_1", "start": 337755, "stop": 338006, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAAGAAAAAGAAATCCAGGTATTTAATGAAGGAAAAACAATTAGAAATTTCACCTATATTGATGATATAGTAGATGGAGTGATCAAGTCTCTAAATTATATTCCAGAAAATCATTACGAAATCTTCAATCTTGGGAATACAAACACCGTTGTACTAAACGATATGATCTCTTATATTGAAAAAGAATGTGGGAAAACTGGAAATAAAAAATATCTCCCTGCTGACTTAGCAGATATTCCCGAAAGCTGA", "aa": "MEEKEIQVFNEGKTIRNFTYIDDIVDGVIKSLNYIPENHYEIFNLGNTNTVVLNDMISYIEKECGKTGNKKYLPADLADIPES", "aa_hexdigest": "d03f3d59ebb64704dec75ea2e3d873ef", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9517.599999999991, "isoelectric_point": 4.485074043273925 }, "id": "NKOCPLAKPK_668", "locus": "GCA003260355_00632" }, { "type": "cds", "sequence": "contig_1", "start": 338814, "stop": 338966, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGCTGTAGTATGATTTTGAGTTGGCTACTTCTTTGCTTGGGGACTAAGCTTGATTTACTTAAAGAAAAAAATATACTTTCAACCAGATTGGAAATTTATATGATCAAATGTTTTAATTATTTCTATATTAACACTTTGTATTCAGAATAG", "aa": "MSCSMILSWLLLCLGTKLDLLKEKNILSTRLEIYMIKCFNYFYINTLYSE", "aa_hexdigest": "30a73f8c288787b67117b26360c09da1", "start_type": "ATG", "rbs_motif": "TTTA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5974.165499999999, "isoelectric_point": 7.6811304092407235 }, "id": "NKOCPLAKPK_669", "locus": "GCA003260355_00633" }, { "type": "cds", "sequence": "contig_1", "start": 339158, "stop": 339322, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGCTTAATTTTTCCGATTGTTGCAGAAGTTATGGAAAAAAAAGATGAGAAAAAACTGAATCTCCTTATTTCATTTTTTTATAGTTATTTTTCTGTTTTTACACTAAGTTTCAGTGCCCTACTCATAGCCCTCGGTCCTGAAATAGCAACTGTTCTTTTTTGA", "aa": "MSLIFPIVAEVMEKKDEKKLNLLISFFYSYFSVFTLSFSALLIALGPEIATVLF", "aa_hexdigest": "c324578e2ce92b92896ec2b881a222c0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6136.300799999997, "isoelectric_point": 5.002479362487793 }, "id": "NKOCPLAKPK_670", "locus": "GCA003260355_00634" }, { "type": "cds", "sequence": "contig_1", "start": 340348, "stop": 341262, "strand": "-", "frame": 1, "gene": "pheS", "product": "phenylalanine--tRNA ligase subunit alpha", "db_xrefs": [ "EC:6.1.1.20", "GO:0000166", "GO:0004826", "GO:0005524", "GO:0005737", "GO:0006432", "KEGG:K01889", "SO:0001217", "UniRef:UniRef50_A0A288DKN3", "UniRef:UniRef90_A0A660MWS4" ], "nt": "ATGATAATGCTCAATTTAGAACAGACCCTTCAGGAGCTCAATGCGATGAATAATCTTGACCAATTAGAAACTTGGTATCAAGAACTTTTAGGAAAAAAAGGAAAGCTGACTGAAGCCTTGAAAGGACTCGCAACACTCAGCCCTGAGGAGAAAAAAGAACAAGGAGGAAAACTTTCCAGCATAAGAACCAGCCTTCAAGACGCCTATCAAGCAAAATTTAACGAACTCAAGACGCTTGAAATCAATGCTCAACTAGAAAAAGAAACGGTAGATATTTCGCTTCCTGGAACTGAACTAGAGGAAGGACATTTTTCTTTGCTTACGAAAGTGAGAAGAGAACTTGAAGAGATCGCTCAGAATATGGGATTTATCGTAGAAAGTGGAACGGATATTGTGAGCAAGTTTGAGAATTTTGAAGCGGTAAATATTCCGGTTTCTCATCCTGCTACTGAAATGCATGATACAATTTATGTGGCGGAGAAAGATGAAAGAGGAGAAAATTATGTTTTGAGAACGCACACCTCTGCAGGTCAGAATTATGTGATTAAAAAATATGGAGTGCCAATCAAAGCGGTTTTGCCAGGAAGAGTATATCGTTTTGAGAATATTGATGCGACGCACGATACGATGTTTTATCAGTTTGAGGGAATCTATATTGATAAGAATATCAGTATCGGACATTTCAAAAGTGTGATTACTAAGTTTCTTTCTGCTGCCCTGCAAAAAGAAGTTGAAATCAGAATGAGACCAGGATTTTTCCCATTCGTAGAGCCTGGTTTTGAGATTGATACGAGATATGAATACACGAATCCTAAGACAGGAGCCAAAGAAATATCCAATCGAATGGAAATCCTCGGCGCTGGAATGATCCATCCTAATGTGCTCAGAGAAGCTGGAATTAACCCTGAAGAATGA", "aa": "MIMLNLEQTLQELNAMNNLDQLETWYQELLGKKGKLTEALKGLATLSPEEKKEQGGKLSSIRTSLQDAYQAKFNELKTLEINAQLEKETVDISLPGTELEEGHFSLLTKVRRELEEIAQNMGFIVESGTDIVSKFENFEAVNIPVSHPATEMHDTIYVAEKDERGENYVLRTHTSAGQNYVIKKYGVPIKAVLPGRVYRFENIDATHDTMFYQFEGIYIDKNISIGHFKSVITKFLSAALQKEVEIRMRPGFFPFVEPGFEIDTRYEYTNPKTGAKEISNRMEILGAGMIHPNVLREAGINPEE", "aa_hexdigest": "832e36113f76e516639d8b21a287e995", "start_type": "ATG", "rbs_motif": "TTTTT", "psc": { "uniref90_id": "UniRef90_A0A660MWS4", "query_cov": 1.0, "subject_cov": 0.8888888888888888, "identity": 0.97, "score": 582.0, "evalue": 6.8e-209, "valid": true, "gene": "pheS", "product": "phenylalanine--tRNA ligase subunit alpha", "ec_ids": [ "6.1.1.20" ], "uniref50_id": "UniRef50_A0A288DKN3", "kegg_orthology_id": "K01889", "go_ids": [ "GO:0000166", "GO:0004826", "GO:0005524", "GO:0005737", "GO:0006432" ] }, "pscc": { "uniref50_id": "UniRef50_A0A288DKN3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A288DKN3" ], "product": "Phenylalanine--tRNA ligase alpha subunit" }, "genes": [ "pheS" ], "id": "NKOCPLAKPK_671", "locus": "GCA003260355_00635" }, { "type": "cds", "sequence": "contig_1", "start": 341386, "stop": 341778, "strand": "+", "frame": 1, "gene": null, "product": "Lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MWM3", "UniRef:UniRef90_A0A660MWM3" ], "nt": "ATGAAAAAGTTGGTTTCTCTTTTAGTGTTTGTTTCTTTCCTTTTTAGTTGTGATTGTGATCGTGTTCTGGATGATCCGGATTTGGCTTGGTCTATTGGGATTGTAAATAATGCAATTCTCAAAGAGCGGACCGTGGCTTTAGAATTTAGGTCTTCGAGACAGGTTCAGAGAGTTGAATTTGAATCATTTTGGAAATTTGAGGTGAATGGATCGATTCTTTCGGTTCGTTTGCTGGATTCAGATTTTACTTGGGAGCAACGAGGGAATGTCCTTCGAGGTGTATGTAAGATTCCTCTTGGGAGCTTGCTTTCTAATCCTCAGGTTCGGCATCGTTATACTCACTTTGTCGGTTATGCGGTGATTGATGGTCGCAGGGTCAGGTTGAGGAATTAG", "aa": "MKKLVSLLVFVSFLFSCDCDRVLDDPDLAWSIGIVNNAILKERTVALEFRSSRQVQRVEFESFWKFEVNGSILSVRLLDSDFTWEQRGNVLRGVCKIPLGSLLSNPQVRHRYTHFVGYAVIDGRRVRLRN", "aa_hexdigest": "44cb5e160da60df8386e8129dbc587b0", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A660MWM3", "query_cov": 1.0, "subject_cov": 0.9285714285714286, "identity": 0.985, "score": 259.0, "evalue": 1.14e-87, "valid": true, "product": "Lipoprotein", "uniref50_id": "UniRef50_A0A660MWM3" }, "pscc": { "uniref50_id": "UniRef50_A0A660MWM3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MWM3" ], "product": "Lipoprotein" }, "genes": [], "id": "NKOCPLAKPK_672", "locus": "GCA003260355_00636" }, { "type": "cds", "sequence": "contig_1", "start": 342555, "stop": 342773, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTAATACAGCGATTTCTGCCTATAAAGGAGAAGATATCGGAGACCTCGCACCTGAAACTTTTGATAAAGTTCTCGTAGACGCCCCTTGTTCCGGGGAAGGGATGCAATATAAGCACGATAAAAAAGTCCAAATGCGAGATCAGAAAGCAGCAGGTAAATTAGCGAAGCTTCAAACCCAGCTTCTAATCTCTGGAATAAAAGCTCTAAAAGTCTGA", "aa": "MLNTAISAYKGEDIGDLAPETFDKVLVDAPCSGEGMQYKHDKKVQMRDQKAAGKLAKLQTQLLISGIKALKV", "aa_hexdigest": "28948a5e0b3150ed50c068ccb2bbe006", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7820.049600000001, "isoelectric_point": 8.760109138488769 }, "id": "NKOCPLAKPK_673", "locus": "GCA003260355_00637" }, { "type": "cds", "sequence": "contig_1", "start": 343068, "stop": 343265, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAACGACCAGTTATCTCGACTTTATTGCTCAGTTCCTTACTAATAAGGACGAGTTTTCCGCATTTAAAGCGTGTTACCAACAAACCATTCCCAAGAGCATCAAACTCATCAATTCAAAAGCAAAAAAAGCTGATCTACTCCCCTATTTTATTGATCAAGGACGGCAACTTGAATATCCAGATCTCTCACATTGA", "aa": "MKTTSYLDFIAQFLTNKDEFSAFKACYQQTIPKSIKLINSKAKKADLLPYFIDQGRQLEYPDLSH", "aa_hexdigest": "ef0f576992220824e9059f1c544420dd", "start_type": "ATG", "rbs_motif": "TTCTA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7563.6152999999995, "isoelectric_point": 8.760044670104982 }, "id": "NKOCPLAKPK_674", "locus": "GCA003260355_00638" }, { "type": "cds", "sequence": "contig_1", "start": 343313, "stop": 343744, "strand": "-", "frame": 3, "gene": "clpB", "product": "ATP-dependent chaperone ClpB", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2T4S4Y8" ], "nt": "ATGGATATGGGAAATATGCTCAAACCAGCCCTCGCCAGAGGACAAATCAGAGTAATCGGAGCAACAACCCTCAATGAATATCGTCTCTACATTGAAAAAGATGCCGCACTTGAGAGAAGATTCCAACCAGTCATGGTAAATGAACCAACTAGAGAAGATGCTCTAGCAATTCTTCGCGGTATTAAAAAAGCCTATGAAACCCACCATGGAGTAAAAATTACTGATGATGCCGTAGTTTCAGCAGTCGACCTATCAAGGAGATATATTCCAGATCGCAAACTCCCAGACAAAGCCATAGATTTATTAGATGAGGCCGCAGCCAGCGTAAAAATGAACCTTGCTACTATGCCAGAAGAAATTCTTCAACTAGAAAAATCTATCAATCAGCTCGAAATCGAAAAACAAGCACTCATAAGGGATGCAGAAAAATAA", "aa": "MDMGNMLKPALARGQIRVIGATTLNEYRLYIEKDAALERRFQPVMVNEPTREDALAILRGIKKAYETHHGVKITDDAVVSAVDLSRRYIPDRKLPDKAIDLLDEAAASVKMNLATMPEEILQLEKSINQLEIEKQALIRDAEK", "aa_hexdigest": "57b6cae5ea71bb3296d774005808d2e8", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00360B1FB4", "query_cov": 0.958041958041958, "subject_cov": 0.85625, "identity": 0.599, "score": 161.0, "evalue": 2.51e-48, "valid": false, "uniref50_id": "UniRef50_A0A2T4S4Y8" }, "pscc": { "uniref50_id": "UniRef50_A0A2T4S4Y8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2T4S4Y8" ], "product": "ATP-dependent chaperone ClpB" }, "genes": [ "clpB" ], "id": "NKOCPLAKPK_675", "locus": "GCA003260355_00639" }, { "type": "cds", "sequence": "contig_1", "start": 343943, "stop": 344194, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0024AE975F", "PFAM:PF13191.12" ], "nt": "ATGAGGAACGGAGAAAAAATCGAAACAAATGATCCAGAAGTCAGCCTTGAAGCTCTTTCTAAATATGGGAAAGATATTACGCTTCTTGCAGAAAAAGGGAAACTTGATCCTGTCATTGGAAGAGATGAAGAACTAAGAAGAGTACTCCAAATCCTTTCTCGTAGGACCAAAAACAATCCCGTCCTTGTCGGAGATCCTGGAGTTGGAAAGACCGCCATTATTGAGCTTCTTGCACAACAAATCGTCAAATGA", "aa": "MRNGEKIETNDPEVSLEALSKYGKDITLLAEKGKLDPVIGRDEELRRVLQILSRRTKNNPVLVGDPGVGKTAIIELLAQQIVK", "aa_hexdigest": "1dc641268e5ccfe98b79bb6ce9b32825", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0024AE975F", "query_cov": 0.8192771084337349, "subject_cov": 0.9714285714285714, "identity": 0.662, "score": 98.2, "evalue": 2.12e-25, "valid": false, "uniref50_id": "UniRef50_UPI0024AE975F" }, "pscc": { "uniref50_id": "UniRef50_UPI0024AE975F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0024AE975F" ] }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13191.12", "name": "AAA_16", "length": 48, "aa_cov": 0.5421686746987951, "hmm_cov": 1.0, "evalue": 2.3786383750155786e-09, "score": 34.371116638183594, "start": 38, "stop": 82 } ], "seq_stats": { "molecular_weight": 9192.556899999996, "isoelectric_point": 8.010083961486817 }, "id": "NKOCPLAKPK_676", "locus": "GCA003260355_00640" }, { "type": "cds", "sequence": "contig_1", "start": 344315, "stop": 344617, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACTTCAATAAACTTACCATTAAAGCCAGCGAGGCAATTCAAGAAGCAAACAGCCTCTGCATCAATAGGGGGAATAATGAAATCACAACAGAACATCTTTTCTTTGCGATGCTCGAACAGACTGATGGCTACCTCCCCTTAATCTTAAAAAAAATGGGGAAAAACATCGAACAGATAAAAGCTGAAACCATTGTACTTTTGGACAGGCTCCCAAGGATTCAAGGGACAAACCAAATCGGACTTAGCAACGAGTTAAACCGCATCTTGGAACAAAGCGAAAGAGAAATGAAAGAGCTCTGA", "aa": "MNFNKLTIKASEAIQEANSLCINRGNNEITTEHLFFAMLEQTDGYLPLILKKMGKNIEQIKAETIVLLDRLPRIQGTNQIGLSNELNRILEQSEREMKEL", "aa_hexdigest": "73c4bbbae71aa081a2ac93c1be8e3b95", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11473.13469999999, "isoelectric_point": 5.424736213684081 }, "id": "NKOCPLAKPK_677", "locus": "GCA003260355_00641" }, { "type": "cds", "sequence": "contig_1", "start": 344870, "stop": 345325, "strand": "-", "frame": 3, "gene": null, "product": "Aminoacyl-transfer RNA synthetases class-II family profile domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A955I2M6" ], "nt": "ATGGGAACTCCGACTTTGATTGATTATTTATTCAAAAAGGTGCTCAGGCCAAAGATCGTAGGACCAGCTTTCATCTATAATTATCCAGTGATTATGCAGCCTTTAGCGAGGACTTCTGATGCTAATGCAAATATCGTAGAGCAGTTCCAGCTCCTCGTAAATGGTTGGGAGATTTGTAAAGCCTATTCTGAACTTGTAGATCCTTTACTCCAACAAGCAAACTTTGACAAACAAGCTGCTGCTGCAGAAAAAGGCGATGATGAAGCGACCAGTGGAGATGATACCTTCGTAGAGGCGATGGAGTATGGAATGCCTCCACAGTCTGGTTTTGGAATGGGACTTGAGAGAATTTTGGCAATTCTGACAGAACAAGAAAACTTGAGAGACGTGGTAATGTTCCCACTTATGAAGCTCAGAAACCAAGATACAGCCTCAGATCCTGAAAAAAATAAATAG", "aa": "MGTPTLIDYLFKKVLRPKIVGPAFIYNYPVIMQPLARTSDANANIVEQFQLLVNGWEICKAYSELVDPLLQQANFDKQAAAAEKGDDEATSGDDTFVEAMEYGMPPQSGFGMGLERILAILTEQENLRDVVMFPLMKLRNQDTASDPEKNK", "aa_hexdigest": "b18d3fe23a66b6fe0b013b533a8b17f2", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A955I2M6", "query_cov": 0.9536423841059603, "subject_cov": 0.9230769230769231, "identity": 0.521, "score": 163.0, "evalue": 7.43e-49, "valid": false, "product": "Aminoacyl-transfer RNA synthetases class-II family profile domain-containing protein", "uniref50_id": "UniRef50_A0A955I2M6" }, "pscc": { "uniref50_id": "UniRef50_A0A955I2M6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A955I2M6" ], "product": "Aminoacyl-transfer RNA synthetases class-II family profile domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_678", "locus": "GCA003260355_00642" }, { "type": "cds", "sequence": "contig_1", "start": 345491, "stop": 346129, "strand": "-", "frame": 3, "gene": "lysS", "product": "lysine--tRNA ligase", "db_xrefs": [ "EC:6.1.1.6", "GO:0004824", "GO:0005524", "GO:0005737", "GO:0006430", "KEGG:K04567", "SO:0001217", "UniRef:UniRef50_A0A288DCF3", "UniRef:UniRef90_A0A839JV47" ], "nt": "ATGACGGCTTACAAATTTATGGAAAAAATGGTGGATCTCGGAGATTTTATCGGAGTAGAAGGAGAAGTCTTCAAAACTCACAAAGGAGAACTCACAATTTTCGTGTCAGCTTTCAAATTCCTTTCCAAAGCGATTAGACCTTTGCCTGAAAAATTCCACGGCTTGAGCGATCAGGAGGAGCTGTATAGGAAAAGATACCTTGATATGACGATGAATCCTGAAACCTATGCGAGATTCCTCTTTAAGTCTAAGTTTTATCAGACTTTAAGGGATTTTTATCGTAAGCATAACTTTATTGAGATTCAGACTCCGATTTTGGATAATGCGGCTTCTGGTGCGGCAGCAAGACCGTTTATTACCCATCATAATGACTATGATACCGATGTTTATCTCAGAATCGCGTTTGAAACCTCACTCAAAAAGGCGACGGTTGGAAGATTTGAGAAAGTGTTTGAGATTGGGCAGGATTTCAGAAATGAAGGTTCTGATCCTTCACATTTGCAGGAGTTTACGCAGGTGGAGCACTATGCCGTGTATTGGAACTATGAGGATAATATGCGTTTTACTGAGGAAATGTTTGATTATCTCTTTGCTGAACTTGGGATTTCTAAGCAGCTTAAAGTAAAAGATAAAGACTGA", "aa": "MTAYKFMEKMVDLGDFIGVEGEVFKTHKGELTIFVSAFKFLSKAIRPLPEKFHGLSDQEELYRKRYLDMTMNPETYARFLFKSKFYQTLRDFYRKHNFIEIQTPILDNAASGAAARPFITHHNDYDTDVYLRIAFETSLKKATVGRFEKVFEIGQDFRNEGSDPSHLQEFTQVEHYAVYWNYEDNMRFTEEMFDYLFAELGISKQLKVKDKD", "aa_hexdigest": "a850c08bc23175b2a56200084257a427", "start_type": "ATG", "rbs_motif": null, "genes": [ "lysS" ], "truncated": "both", "pseudogene": { "start": 345188, "stop": 346429, "observations": { "mutation": [ 345493, 346135 ], "stop": [ 345493, 346135 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A839JV47", "query_cov": 1.0, "subject_cov": 0.4007561436672968, "identity": 0.91, "score": 399.0, "evalue": 1.21e-135, "gene_start": 1, "gene_end": 212, "reference_start": 112, "reference_end": 323, "reference_sequence": "MKKMGIHPYAQSFQKKDLISDIIAQYEAQTLRDSEEVVLNPQVQVQTAGRVMLYRSHGKLAFAKVLDSSNQIQLMFHRDHCKILKTITNSDGKSEDVAVDVLENADTESGELSAYKFMEKMVDLGDFIGVKGEVFKTHKGELTIFVSEFKFLSKAIRPLPEKFHGLNDQEELYRKRYLDMTMNPETYQRFLFKSKFYQVLRDFYRSRGFTEIQTPILDNAASGAAARPFITHHNDFDTDVYLRIAFETSLKKATVGRFEKVFEIGQDFRNEGSDPSHVQEFTQVEHYAVYWNYEDNMKFTEEMFDYLFEHLGLEKKLKVKDKDGIEKTVDFTTPWAKIDYTQGVNEASGLDISKYGIDDADKLRADIQAKGITFEGMENMGTTTLIDYLYKKVLRPKIVGPAFIYNYPVIMQPLARVSDADEQIVEQFQLLVNGWEICKAYSELVDPILQQENFDLQAEASKKGDDEATSGDDAFVEAMEYGMPPQSGFGMGLERILAILTEQENLRDVVMFPLMKARNQDITSEEEEK" }, "paralog": false, "identity": 0.8038740920096852, "score": 676.0, "evalue": 7.69e-247, "description": "Internal stop codon at 345493, 346135. Nonsense mutation around 345493, 346135.", "psc": { "uniref90_id": "UniRef90_A0A839JV47", "gene": "lysS", "product": "lysine--tRNA ligase", "ec_ids": [ "6.1.1.6" ], "uniref50_id": "UniRef50_A0A288DCF3", "kegg_orthology_id": "K04567", "go_ids": [ "GO:0004824", "GO:0005524", "GO:0005737", "GO:0006430" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A839JV47", "EC:6.1.1.6", "UniRef:UniRef50_A0A288DCF3", "KEGG:K04567", "GO:0004824", "GO:0005524", "GO:0005737", "GO:0006430" ] }, "pscc": { "uniref50_id": "UniRef50_A0A288DCF3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A288DCF3" ], "product": "Lysine--tRNA ligase" } }, "id": "NKOCPLAKPK_679", "locus": "GCA003260355_00643" }, { "type": "cds", "sequence": "contig_1", "start": 346133, "stop": 346516, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01336.31" ], "nt": "ATGGCACAAGTTCAACTTAGCGAAAGAGAGGTCAGAATACAGAAAACTCAGAAACTTCAAGCGATGGGAATTCATCCTTATGCTCAATCTTTTCACAAAACGCACCTCATCAAAGATATTATCGCGACTTATGAAAATCAGCAGCTTCGCGAGATTGAGGCGATTATCGAAGCTCCAAAAGTGCAGGTCAAAACTGCTGGAAGAGTGATGCTCTATCGTTCGCATGGGAAGCTTGCTTTTGCAAAAATCCTTGATAGCACCGAGCAAATTCAGCTGATGTTTCATAGAGATCAGTGCAAGATTATCAAAACAGAAAAAAAAGCTGATGGAAGCATGGAAGATATTGAGGTTCAGAGTTTAGACTTTCCAGAGAGTGAAACCTGA", "aa": "MAQVQLSEREVRIQKTQKLQAMGIHPYAQSFHKTHLIKDIIATYENQQLREIEAIIEAPKVQVKTAGRVMLYRSHGKLAFAKILDSTEQIQLMFHRDQCKIIKTEKKADGSMEDIEVQSLDFPESET", "aa_hexdigest": "7c5540b0affd3625917c5002bcd3ed3d", "start_type": "ATG", "rbs_motif": "TTTTT", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01336.31", "name": "tRNA_anti-codon", "length": 38, "aa_cov": 0.28346456692913385, "hmm_cov": 1.0, "evalue": 7.772069444128044e-08, "score": 28.6664981842041, "start": 63, "stop": 98 } ], "seq_stats": { "molecular_weight": 14667.757299999988, "isoelectric_point": 6.971097755432129 }, "id": "NKOCPLAKPK_680", "locus": "GCA003260355_00644" }, { "type": "cds", "sequence": "contig_1", "start": 346688, "stop": 346900, "strand": "+", "frame": 2, "gene": null, "product": "DUF192 domain-containing protein", "db_xrefs": [ "KEGG:K09005", "SO:0001217", "UniRef:UniRef50_A0A2W4J0W1", "UniRef:UniRef90_A0A328EI81" ], "nt": "ATGCTCAATTCAACTTCAGAGAAAAAGCAATTCTTTCAAAGTCTCATTAGACGAGTAATGATTTTAATTGGGATCAAAGTTCTTATTATCAGCTTTTTCTTTTTTCACTTTCTAGAAAAGAATAAACTCAGTCCTCAAAAACCTCAAATGTGTTATGAAAATCACTGTTTTGAGCTTGAAATTGCTGATACACCAGAAAAGAGAGAACTCTGA", "aa": "MLNSTSEKKQFFQSLIRRVMILIGIKVLIISFFFFHFLEKNKLSPQKPQMCYENHCFELEIADTPEKREL", "aa_hexdigest": "861313cb08095248f1c29de7f1e6324a", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 346688, "stop": 347185, "observations": { "mutation": [ 346898 ], "stop": [ 346898 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EI81", "query_cov": 1.0, "subject_cov": 0.42424242424242425, "identity": 0.986, "score": 135.0, "evalue": 2.37e-39, "gene_start": 1, "gene_end": 70, "reference_start": 1, "reference_end": 70, "reference_sequence": "MLNSTSEKKQFFQSLIRRVMILIGIKVLIISFFFFHFLEKSKLSPQKPQMCYENHCFELEIADTPEKRELGLMFRESLAENHGMLFVFDRSDFWSFWMRNTLIPLDIVRLDTGNRVVDTASAAPCEKMPCQNYFPRSEAIQAIELNSGTLEKLGIQIGQELKIIK" }, "paralog": false, "identity": 0.9636363636363636, "score": 320.0, "evalue": 2.62e-114, "description": "Internal stop codon at 346898. Nonsense mutation around 346898.", "psc": { "uniref90_id": "UniRef90_A0A328EI81", "product": "DUF192 domain-containing protein", "uniref50_id": "UniRef50_A0A2W4J0W1", "kegg_orthology_id": "K09005", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EI81", "UniRef:UniRef50_A0A2W4J0W1", "KEGG:K09005" ] }, "pscc": { "uniref50_id": "UniRef50_A0A2W4J0W1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4J0W1" ], "product": "DUF192 domain-containing protein" } }, "id": "NKOCPLAKPK_681", "locus": "GCA003260355_00645" }, { "type": "cds", "sequence": "contig_1", "start": 346904, "stop": 347185, "strand": "+", "frame": 2, "gene": null, "product": "DUF192 domain-containing protein", "db_xrefs": [ "KEGG:K09005", "SO:0001217", "UniRef:UniRef50_A0A2W4J0W1", "UniRef:UniRef90_A0A328EI81" ], "nt": "ATGTTTCGTGAATCACTTCCAGAAAATCAGGGGATGCTCTTTGTTTTTGATAGATCAGACTTTTGGAGTTTTTGGATGAGAAATACCTTGATTCCGCTTGATATTGTCCGATTAGATACGGGGAATAGAGTTGTGGATTTCGTTTCTGCAGCTCCGTGTGAGAAGATGCCGTGTCAGAATTATTTTCCGAGGTCTGAAGCGATCCAAGCGATCGAACTTAACAGCGGCACATTGGAGAAACTGGGGATTCAAATTGGTCAGGAGCTTAAGATTATTAAATAG", "aa": "MFRESLPENQGMLFVFDRSDFWSFWMRNTLIPLDIVRLDTGNRVVDFVSAAPCEKMPCQNYFPRSEAIQAIELNSGTLEKLGIQIGQELKIIK", "aa_hexdigest": "e7836a242b116f23700c41cffb735775", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 346688, "stop": 347185, "observations": { "mutation": [ 346898 ], "stop": [ 346898 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EI81", "query_cov": 1.0, "subject_cov": 0.5636363636363636, "identity": 0.9570000000000001, "score": 182.0, "evalue": 2.43e-57, "gene_start": 1, "gene_end": 93, "reference_start": 73, "reference_end": 165, "reference_sequence": "MLNSTSEKKQFFQSLIRRVMILIGIKVLIISFFFFHFLEKSKLSPQKPQMCYENHCFELEIADTPEKRELGLMFRESLAENHGMLFVFDRSDFWSFWMRNTLIPLDIVRLDTGNRVVDTASAAPCEKMPCQNYFPRSEAIQAIELNSGTLEKLGIQIGQELKIIK" }, "paralog": false, "identity": 0.9636363636363636, "score": 320.0, "evalue": 6.21e-114, "description": "Internal stop codon at 346898. Nonsense mutation around 346898.", "psc": { "uniref90_id": "UniRef90_A0A328EI81", "product": "DUF192 domain-containing protein", "uniref50_id": "UniRef50_A0A2W4J0W1", "kegg_orthology_id": "K09005", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EI81", "UniRef:UniRef50_A0A2W4J0W1", "KEGG:K09005" ] }, "pscc": { "uniref50_id": "UniRef50_A0A2W4J0W1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4J0W1" ], "product": "DUF192 domain-containing protein" } }, "id": "NKOCPLAKPK_682", "locus": "GCA003260355_00646" }, { "type": "cds", "sequence": "contig_1", "start": 347373, "stop": 347843, "strand": "+", "frame": 3, "gene": null, "product": "tRNA-specific adenosine deaminase", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBFAABD", "UniRef:UniRef100_A0A328EGK2", "UniRef:UniRef50_A0A090ZKS6", "UniRef:UniRef90_A0A328EGK2" ], "nt": "ATGACTCACGAATACTTTATGGAGAAGGCGGTAGAGTTGTCCCTAGAAAATATGAGAGCAGGGAAAGGTGGACCCTTTGGTGCGATCATTGTGAGGCAAGGGAAGATTGTTGGAACGGGGGCTAATCATGTCACTTCTGACAATGACCCTACGGCACATGCTGAGGTGGTAGCGATCAGAGATGCTTGCAAGAACTTGGGGACGTTTCAGCTAGATGATTGTGAGATTTACACAAGTTGTGAACCTTGTCCGATGTGTCTTGCAGCGATTTATTGGGCGAGACCAAAGGCGATTTACTATGCCAATACGAGAAAAGATGCTGCAGATATCGGGTTTGATGATGACTTCCTTTATGAAGAGCTCAAAAAGAACCTCGAAGAAAGAAGTCTTCCTATCCATCAGCTTGATAAAAGCAATGCACTCAAGGTTTTTGATGAATGGAAGAGAAAAACTGACAAGATTGAGTACTAA", "aa": "MTHEYFMEKAVELSLENMRAGKGGPFGAIIVRQGKIVGTGANHVTSDNDPTAHAEVVAIRDACKNLGTFQLDDCEIYTSCEPCPMCLAAIYWARPKAIYYANTRKDAADIGFDDDFLYEELKKNLEERSLPIHQLDKSNALKVFDEWKRKTDKIEY", "aa_hexdigest": "19ef04ddbe1dc116bba10dd42815a2ff", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000DBFAABD", "uniref100_id": "UniRef100_A0A328EGK2", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBFAABD", "UniRef:UniRef100_A0A328EGK2" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EGK2", "uniref90_id": "UniRef90_A0A328EGK2" }, "psc": { "uniref90_id": "UniRef90_A0A328EGK2", "product": "tRNA-specific adenosine deaminase", "uniref50_id": "UniRef50_A0A090ZKS6" }, "pscc": { "uniref50_id": "UniRef50_A0A090ZKS6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A090ZKS6" ], "product": "Guanine deaminase" }, "genes": [], "id": "NKOCPLAKPK_683", "locus": "GCA003260355_00647" }, { "type": "cds", "sequence": "contig_1", "start": 347880, "stop": 348200, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGAAAACCTGTATTTTCAGTCCCAGCTCCTATCTTTTCACCTCAAAGTGCCGGCGTCTTTGCCAAAATGAACGAACAAAAAATCCAACTCATCACAGACTTTGACGCCTGCTTACAAAAACACTTTCCTCAAAAAGGAGCAGTATTGCAAATAGAAAAAACATCACTAACCGACCGTCAAATCGAGCTCCTACAACATCTCGAAAAACAGGGGGATTCCAGCTTAGAAAGTCTGCTTGCTTACACACATCTCAGCTACGGAGAACTCCTCCAAGAGCTAACCTTCTTAGAAATAGATAAGCAAATCCAAGAAATCTGA", "aa": "MGKPVFSVPAPIFSPQSAGVFAKMNEQKIQLITDFDACLQKHFPQKGAVLQIEKTSLTDRQIELLQHLEKQGDSSLESLLAYTHLSYGELLQELTFLEIDKQIQEI", "aa_hexdigest": "d3b8787a6d87885b3d72b71e231a3cbd", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11973.623999999994, "isoelectric_point": 5.043119239807129 }, "id": "NKOCPLAKPK_684", "locus": "GCA003260355_00648" }, { "type": "cds", "sequence": "contig_1", "start": 348420, "stop": 348668, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACGACTCCCAAGCTCCTTTTTTGCTCTACTACCAAGGGAATCTTTCTCTCCTCGAGGAAAAAATTCTCGGAATAGTAGGACCAAGACAGCCCAGTGAATATGGAATTCAGGTTTTGGAACTTTTTTTTGAAAAAGCAAGCAACTTTCAGCTTGCTTCGCTCTCAGGATTTGCAAAAGGAGTGTATCAAAAAACACATCAACTCTCACTACTGCACAACATCCCAACCATCGCAGTGCTCGGATGA", "aa": "MNDSQAPFLLYYQGNLSLLEEKILGIVGPRQPSEYGIQVLELFFEKASNFQLASLSGFAKGVYQKTHQLSLLHNIPTIAVLG", "aa_hexdigest": "fa82e5208cdd3cd26e0c2ae85de2dc3d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9096.375499999993, "isoelectric_point": 6.022000312805175 }, "id": "NKOCPLAKPK_685", "locus": "GCA003260355_00649" }, { "type": "cds", "sequence": "contig_1", "start": 348956, "stop": 349399, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EJR0", "UniRef:UniRef90_A0A328EJR0" ], "nt": "TTGATTTTTGGTTATGAAATCACTATTATCAATGTTGTCAATAATGACATAATAACAAAAAAGATAAATAATATGAACTGGAGAAAAGAAAAAAGAAGCCTTCTTAATCAGGTGAGGCTCAGTGTACAACGTGAGATAAGGTTTTCAGGGAAAACGGCGAAGCAGCGTATCAAGTTTTTACAGAACTTGAGTCGTAGTTCTTGTGACTTGGAAAATCCAACATTTGGTAAAGTTAGACCGGGTGCATTGGTGGCTCTCAAAACCGCCGAAACTGTGACTTGGCTCTTTATTCTGCCGTTTGGATTTGATGCTTCTTACAAGCTACGAAATGAAACTCTCAAAGAGGTTGAAGTCATTTCAATCTTTGATGAGCGGTTTAAATTTCTGTGTGAGAGGTCTTCCGGAGCCTTGGTAGAGATTGGAGAAATTGTCCAAGTTCACTGA", "aa": "MIFGYEITIINVVNNDIITKKINNMNWRKEKRSLLNQVRLSVQREIRFSGKTAKQRIKFLQNLSRSSCDLENPTFGKVRPGALVALKTAETVTWLFILPFGFDASYKLRNETLKEVEVISIFDERFKFLCERSSGALVEIGEIVQVH", "aa_hexdigest": "7ab92b834bc60c2e034181b4d00f3c25", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 348911, "stop": 349486, "observations": { "mutation": [ 348941, 348944, 349397 ], "stop": [ 348941, 348944, 349397 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EJR0", "query_cov": 1.0, "subject_cov": 0.7696335078534031, "identity": 0.993, "score": 285.0, "evalue": 1.13e-96, "gene_start": 1, "gene_end": 147, "reference_start": 16, "reference_end": 162, "reference_sequence": "MVFFFCQKKFGGLFVLIFGYEITIINVVNNDIITKKINNMNWRKEKRSLLNQVRLSVQREIRFSGKTAKQRIKFLQNLSRSSCDLENPTFGKVRPGALVALKTAETVTWLFILPFGFDASYKLRNETLKEVEVISIFDERFKFLCERSSGALVEIGEIVQVHGWSNHVKPLCVLKPLCVFAEWFFKIILLG" }, "paralog": false, "identity": 0.9790575916230366, "score": 373.0, "evalue": 8.27e-134, "description": "Internal stop codon at 348941, 348944, 349397. Nonsense mutation around 348941, 348944, 349397.", "psc": { "uniref90_id": "UniRef90_A0A328EJR0", "uniref50_id": "UniRef50_A0A328EJR0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EJR0", "UniRef:UniRef50_A0A328EJR0" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EJR0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EJR0" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 16996.597599999994, "isoelectric_point": 9.649579429626463 }, "id": "NKOCPLAKPK_686", "locus": "GCA003260355_00650" }, { "type": "cds", "sequence": "contig_1", "start": 349734, "stop": 349919, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATCAGTGCGTTTTCTAATGATGGCTTAGCAGACGTCGAAGGTATTGGGGAAAAATCGCTACAAGCAATTCAGCTTTTTATGACGAATCCCGAAACCTTAAAGCTTCTGCGTAGCTTAGAACAAGAAGGCGTACAATTCTCCGCAATAAATGAAAAAACAAATCCTGAATCCTCATCACACTGA", "aa": "MISAFSNDGLADVEGIGEKSLQAIQLFMTNPETLKLLRSLEQEGVQFSAINEKTNPESSSH", "aa_hexdigest": "af78962ab60f1d2ea27a0982407c6df4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6652.343499999999, "isoelectric_point": 4.478537559509278 }, "id": "NKOCPLAKPK_687", "locus": "GCA003260355_00651" }, { "type": "cds", "sequence": "contig_1", "start": 349998, "stop": 350402, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATTCCTTACATTTTGGGAAGCATCAAAGAACGTAGAAATGGGAGTGAAGAGCTGATTATTCCTCCTCTATTCTGTCCTGAATGCGGAGATCCGATTACCAATATAGATATTCACTACTATTGCACCAATCCCTCTTGTCCAGCACAAATAAGAGAAAAGATTACGCATTTTGTTTCGAGAGATGCGATGGATATTAGTGGAATTGGTGAAGCGATGATTGAGGTCTTAGTCAAACAAAAAATGCTGAATTCAGTCGCAGATATCTACCACCTGACTACGCTTCAAAATCAGATTCTCCTGCGTAAATTCCCAAGCTTTGGAGAAAAGAAAATCACCGAGCTTGTCAATCAAATTGAACGTTCAAAAAAACAACCACTCTGGAGACTTCTCAATGCCCTTTGA", "aa": "MIPYILGSIKERRNGSEELIIPPLFCPECGDPITNIDIHYYCTNPSCPAQIREKITHFVSRDAMDISGIGEAMIEVLVKQKMLNSVADIYHLTTLQNQILLRKFPSFGEKKITELVNQIERSKKQPLWRLLNAL", "aa_hexdigest": "541d7888608c5a20babb9c17c224352c", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15353.824199999994, "isoelectric_point": 7.673798179626465 }, "id": "NKOCPLAKPK_688", "locus": "GCA003260355_00652" }, { "type": "cds", "sequence": "contig_1", "start": 350646, "stop": 350924, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGATCTCAAGTCGCTTGGAATGCCCGTTTTTGAGTTGCCAGAAAACTTCCTTGTGACTACAGATATTGAGCAAATTATCACTTGGTGTCAGGATTTTCAAATCAAGCACTTTTTGGAGGAGCAAGATTTTGATTTTGATGGCTTGGTAATTAAGGTTTCTGATAGTAAAACTCCTCAAAATACGTCAAACTGCAATACCTCAGAAAAATCTGACTTTTCTCTTTTTGATACAGAGACTCCAGTAAATAGTAGCGAAGACATTCGTTCAGCACTCTGA", "aa": "MDLKSLGMPVFELPENFLVTTDIEQIITWCQDFQIKHFLEEQDFDFDGLVIKVSDSKTPQNTSNCNTSEKSDFSLFDTETPVNSSEDIRSAL", "aa_hexdigest": "36da95f9c03c154df233f5e0b659eabd", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10512.53179999999, "isoelectric_point": 4.0500284194946286 }, "id": "NKOCPLAKPK_689", "locus": "GCA003260355_00653" }, { "type": "cds", "sequence": "contig_1", "start": 350934, "stop": 351119, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01653.23" ], "nt": "TTGCAGGTTAGAGGAGAGATTATGATGCCAAAATCCCAGCTCCTCAAGCTCAATCAGCAAAGAGACCACTCCGGTCAAACCCCCTTTTCAAATACGAGAAATGCCGCTGCTGGAAGCATAAAACTCCTAGATAGCAGAGAAGTTGCGAAAAGAGGCTTGATTGCTTTCATATATGATCAGCTCTGA", "aa": "MQVRGEIMMPKSQLLKLNQQRDHSGQTPFSNTRNAAAGSIKLLDSREVAKRGLIAFIYDQL", "aa_hexdigest": "a5391b1807df11e6d86d8b325647c479", "start_type": "TTG", "rbs_motif": "TTCTT", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01653.23", "name": "DNA_ligase_aden", "length": 58, "aa_cov": 0.9508196721311475, "hmm_cov": 1.0, "evalue": 1.842782255231684e-18, "score": 63.379024505615234, "start": 2, "stop": 59 } ], "seq_stats": { "molecular_weight": 6872.8693, "isoelectric_point": 10.15120792388916 }, "id": "NKOCPLAKPK_690", "locus": "GCA003260355_00654" }, { "type": "cds", "sequence": "contig_1", "start": 351123, "stop": 351737, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01653.23", "PFAM:PF22745.1" ], "nt": "TTGCTTTTCTCTAAGCTAAGATCAGACTTTACTCTTTTCCTAATACTCGTGAATCTCTCAGAATCTACTCAATACCTTCAATCTTTCATCAAAAAAGTTCCAAATCCTGAACATATCCCCGATTCAGAATTGAAAACGATCTACCAGCTTTTCATCGATACTTTAACCGATCATAATCATCTCTACTATATTGAGGCAAAGCCAATCATCTCTGATGCAGAATATGATGAACTTTTCGCCTATCTCAAAGCAATGGAAGAATACTTCCCGACGATCATTTCCAGCACCTCTCCAACGCAAGGTCTAGTAGGACAACTTTCTGACGGCTTCAAAAAGGCAGTCCATCAAATCCCACTTCTTTCCCTAGAAAATAGTTATAATGCAGAGGATATTAGAGAATGGGCAGTCAGAGTTGCAAAGTTGGCAGAAAAAAAAGGAATTCTGAACCGGGAATATCATTTAGAGCCAAAATTTGATGGACTCAGCGTAGAATGCATTTATCATGATGGGAAACTCACCCAAGCAATCACCAGAGGCGATGGGAAAATTGGGGAAGACATTACCGCGAATGTGATGATGATTCCTTCAATTCCAAAGCAGATTTCTTATCTTTGA", "aa": "MLFSKLRSDFTLFLILVNLSESTQYLQSFIKKVPNPEHIPDSELKTIYQLFIDTLTDHNHLYYIEAKPIISDAEYDELFAYLKAMEEYFPTIISSTSPTQGLVGQLSDGFKKAVHQIPLLSLENSYNAEDIREWAVRVAKLAEKKGILNREYHLEPKFDGLSVECIYHDGKLTQAITRGDGKIGEDITANVMMIPSIPKQISYL", "aa_hexdigest": "c8876afd95970cb8687e6aced19a76b6", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01653.23", "name": "DNA_ligase_aden", "length": 94, "aa_cov": 0.46078431372549017, "hmm_cov": 0.9787234042553191, "evalue": 1.6127595629586403e-29, "score": 99.60857391357422, "start": 109, "stop": 202 }, { "id": "PF22745.1", "name": "Nlig-Ia", "length": 43, "aa_cov": 0.2107843137254902, "hmm_cov": 1.0, "evalue": 4.132006053557356e-07, "score": 27.053213119506836, "start": 54, "stop": 96 } ], "seq_stats": { "molecular_weight": 23292.427600000014, "isoelectric_point": 5.244954490661621 }, "id": "NKOCPLAKPK_691", "locus": "GCA003260355_00655" }, { "type": "cds", "sequence": "contig_1", "start": 352105, "stop": 352395, "strand": "-", "frame": 1, "gene": null, "product": "Phosphoesterase HXTX domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EKC1", "UniRef:UniRef90_A0A328EKC1" ], "nt": "ATGTTCTATACCATAGTTGCACCTCCTCAGGATGATCAATTACATAAACTGAATACGATCAGAAATTATTTTTACCAAAATGGCTTTAGACAGAGAGTTTCCAAATGCAAAGAAAACGCACATATTACCTTATTACAGTTAAAGGATTCTTCATCTAAAAATTTTTGGTCAGAAGTTGAAAGTAGTCTCATCAATACTAGAAAAATAAACCTGACTAACTTTCAGATAATCCTCCAAGAGCACGATCGAATCAAAAAAAATCCAGAACGAAACAAAAAATATCCTGAATGA", "aa": "MFYTIVAPPQDDQLHKLNTIRNYFYQNGFRQRVSKCKENAHITLLQLKDSSSKNFWSEVESSLINTRKINLTNFQIILQEHDRIKKNPERNKKYPE", "aa_hexdigest": "320f1b082824b6a6fdd0d365188be6df", "start_type": "ATG", "rbs_motif": "TTATT", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 351802, "stop": 352395, "observations": { "mutation": [ 351972, 352107 ], "stop": [ 351972, 352107 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EKC1", "query_cov": 1.0, "subject_cov": 0.463768115942029, "identity": 1.0, "score": 193.0, "evalue": 5.72e-61, "gene_start": 1, "gene_end": 96, "reference_start": 1, "reference_end": 96, "reference_sequence": "MFYTIVAPPQDDQLHKLNTIRNYFYQNGFRQRVSKCKENAHITLLQLKDSSSKNFWSEVESSLINTRKINLTNFQIILQEHDRIKKNPERNKKYPEGCGRFALFFPKNQNLIHTAKKIRKIAENFDVDDSLAYAQNIANATGEYRDPQDIFNYLANHMNLCNYIRLEKMHLAATYFKQHFNIQSLIIDKIALAENKGQLVNISYLKK" }, "paralog": false, "identity": 0.9898477157360406, "score": 397.0, "evalue": 7.84e-144, "description": "Internal stop codon at 351972, 352107. Nonsense mutation around 351972, 352107.", "psc": { "uniref90_id": "UniRef90_A0A328EKC1", "product": "Phosphoesterase HXTX domain-containing protein", "uniref50_id": "UniRef50_A0A328EKC1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EKC1", "UniRef:UniRef50_A0A328EKC1" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EKC1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EKC1" ], "product": "Phosphoesterase HXTX domain-containing protein" } }, "id": "NKOCPLAKPK_692", "locus": "GCA003260355_00656" }, { "type": "cds", "sequence": "contig_1", "start": 352528, "stop": 352977, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTTAAAAAATCAATATATGGCTTTTACTTAGCGACACTTCTTCAAAATGCATTAAAGGTCATTACTCCTTTCTATATTATTTATTTTGTAGCTTTAGGATTTAGCTTTTGGCAAATTGCCTTGATTTCTAGTTTAAGAAGCATTGTTGGACTTATTTTTGAGATTCCAACAGGAATGATTGCTGATATTTATGGGAAGAAAACGAGTGTTATCCTTGGTTATAGTCTTTCTGCTCTCACTTTGTTTCTGATTCCACTTACCGATAATTTTCTTTGGATTGCGGTTGTTTTCTGTTTTAATGCTTTTTTTGAAACTTTTTTTACAGGGGCTGATAATGCTTGGGTTTCTGATAGAATAGAAGCTGAAAATCCTGATGATATGAAGTCATTTTTTATGAGGAAAATGAGTTTAAGAAATATTGGATTTATCGTTGCTGGGCTTTTGTGA", "aa": "MFKKSIYGFYLATLLQNALKVITPFYIIYFVALGFSFWQIALISSLRSIVGLIFEIPTGMIADIYGKKTSVILGYSLSALTLFLIPLTDNFLWIAVVFCFNAFFETFFTGADNAWVSDRIEAENPDDMKSFFMRKMSLRNIGFIVAGLL", "aa_hexdigest": "af044d5dd631d5c238bf5541451f1646", "start_type": "GTG", "rbs_motif": "TTATT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16908.84159999998, "isoelectric_point": 7.797660636901856 }, "id": "NKOCPLAKPK_693", "locus": "GCA003260355_00657" }, { "type": "cds", "sequence": "contig_1", "start": 353329, "stop": 353595, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTTGAAAAGGTTCTTAAGAAAAAGAAAAATAGTCCTCTTGTTTTGGGTACTTCACTCTTGATCTTTGTAGGAATTCTTATCTTCGCAGGATTGACTTCCTCAATTACAATGCTCATTTGCCTCTACATTATCTACAATTTTATTGATGATATTATTTTCCCTGTAGATGAGCTATTGACGAATCAGCTTATCTCAGCAGAAAAAAGGGCAACCATTTTATCTATGAAATCAGTTGTTGAAAATTTGAGTTCAATTCTTGGTTGA", "aa": "MLEKVLKKKKNSPLVLGTSLLIFVGILIFAGLTSSITMLICLYIIYNFIDDIIFPVDELLTNQLISAEKRATILSMKSVVENLSSILG", "aa_hexdigest": "6b0af0445a00e6d14cd8332449217224", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9730.67829999999, "isoelectric_point": 7.8434331893920906 }, "id": "NKOCPLAKPK_694", "locus": "GCA003260355_00658" }, { "type": "cds", "sequence": "contig_1", "start": 354774, "stop": 355091, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTTTGCATCTCCCAGTAGAAATTATAGAAAGGCACCGTCATAGTTATGATCTGTTTCCAGATTCTGGGAGAGTCTTGCCCTTTGCTTCAGGTGCAACAGTTTTTTACAATTATGTAGATCTCAAGATAAAAAATACTTTGGAATACCCGATTCAAATCAAACTTTGGACTGATGAGAAATATCTCAAGGGACAAATCCGCTCGCCTCAAGCCAAAGAAGTGAAAATTCATGTTTTTGAAGAAGATCATTGCTTTATTCGTTATCAAGGGACATTCTGGAGATACAATACCATCAAAAAAACAAATAAAAAATGA", "aa": "MFLHLPVEIIERHRHSYDLFPDSGRVLPFASGATVFYNYVDLKIKNTLEYPIQIKLWTDEKYLKGQIRSPQAKEVKIHVFEEDHCFIRYQGTFWRYNTIKKTNKK", "aa_hexdigest": "3a1429344cadb5cc849fd1621910f566", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12678.483899999988, "isoelectric_point": 9.35141315460205 }, "id": "NKOCPLAKPK_695", "locus": "GCA003260355_00659" }, { "type": "cds", "sequence": "contig_1", "start": 355128, "stop": 355553, "strand": "-", "frame": 2, "gene": null, "product": "Vancomycin resistance protein", "db_xrefs": [ "KEGG:K18346", "SO:0001217", "UniRef:UniRef50_A0A2W4KR14", "UniRef:UniRef90_A0A2W4KRH1" ], "nt": "ATGCCTCTACTTCATAACCCTCAATTAGCTGAGCATATCAAAACACTGCTTCAAGAGCAGAAAAAGATCAAAACTTCCCGAAGTATGAAGTTTCCGCTTGTGATGCCATTTTTTGTCCGAATAAAACAATGGATAAGGAGAATTGAAAATGTGTTTCAGCCAATTGCAAGGGGAAGATCAGATTTTTTACCTTATATCAATGTAAAACATCAATCCGTTTTACTCAGAAAGCTGGGCGATGTGGATATGCAGCTTCAAGTCAATAAAGTAAAAAATCTTCAAATCGCTTTAGAAAAAATTAATGGAATTCTGATTCAGCCTGGAGAAATTTTTTCTTTTTGGGAATTGGTGAGAAAACCCACTGCCCAAAAGGGCTATCTAGAGGGGATCTTGATTTCAAAAGGAAAGGTAAAAGTAGGAATCTGA", "aa": "MPLLHNPQLAEHIKTLLQEQKKIKTSRSMKFPLVMPFFVRIKQWIRRIENVFQPIARGRSDFLPYINVKHQSVLLRKLGDVDMQLQVNKVKNLQIALEKINGILIQPGEIFSFWELVRKPTAQKGYLEGILISKGKVKVGI", "aa_hexdigest": "dec8790d170001ad91697f009ba912a5", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 354825, "stop": 355616, "observations": { "mutation": [ 355130, 355607 ], "stop": [ 355130, 355607 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A2W4KRH1", "query_cov": 1.0, "subject_cov": 0.44339622641509435, "identity": 0.887, "score": 255.0, "evalue": 3e-83, "gene_start": 1, "gene_end": 141, "reference_start": 22, "reference_end": 162, "reference_sequence": "MLSGGESWKSFNICFNIVSLAMPVLHNPQLAEHIKTLFQEQKKIKTSRSMKFPLLMPFFVRIKQWIRRIENVFQPIARKKSAFLPFINVRHQSVLLRKLGDVDMELQVNKVKNLEIALEKINGILIQPGEIFSFWELVGEPTAKKGYLDGVLISKGKVKVGVGGGLCQLGNLLYWMFLHLPVEMIERHRHSYDLFPDSGRVLPFASGATVFYNYVDLKIKNTLEYPIQIKLWTDEKYLKGQILSPQAKEEKIHIFEEDHCFIRYQERFWRYNRIKKLVQKSDTSETTLISENLVPVMYQVNLETLLEKYEVLDIDAGK" }, "paralog": false, "identity": 0.8859315589353612, "score": 484.0, "evalue": 6.79e-176, "description": "Internal stop codon at 355130, 355607. Nonsense mutation around 355130, 355607.", "psc": { "uniref90_id": "UniRef90_A0A2W4KRH1", "product": "Vancomycin resistance protein", "uniref50_id": "UniRef50_A0A2W4KR14", "kegg_orthology_id": "K18346", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A2W4KRH1", "UniRef:UniRef50_A0A2W4KR14", "KEGG:K18346" ] }, "pscc": { "uniref50_id": "UniRef50_A0A2W4KR14", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4KR14" ], "product": "Vancomycin resistance protein" } }, "id": "NKOCPLAKPK_696", "locus": "GCA003260355_00660" }, { "type": "cds", "sequence": "contig_1", "start": 355570, "stop": 355935, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTGGGCAGGGGGCGATTGGGAACCCTCGGGTTTTTACGCCTCACGAGCCGACTTTGGAGGAGAAGATTGAAGTGATCAAAGCACATTTGGAAGTGATGATTGCCTGTGAGTTGCGATTTGATTACCGAGGAGAAAAAGTAAAAGATTATAGGTTCAATCAACCAAAAAAATCAGATTTAGAATCTTTAAAAAAAGAAATCAATTCTGAAGCTGAATATCGTTCAGTAATTGAATTTAGAAAGTATCTTTTCCAGTATATCAAAGGCATTCCCAACTCTCGTGAATGGAAGCAAGAAGTTATTCCAGTCAAGACCTATGATGAATTGATGGAAAAATTGGAAAAGCTTCAACATATGCTTTAA", "aa": "MVGQGAIGNPRVFTPHEPTLEEKIEVIKAHLEVMIACELRFDYRGEKVKDYRFNQPKKSDLESLKKEINSEAEYRSVIEFRKYLFQYIKGIPNSREWKQEVIPVKTYDELMEKLEKLQHML", "aa_hexdigest": "0fb4e563f8663887e9fd7ebc6b5d2bc0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14432.534199999995, "isoelectric_point": 6.846734046936036 }, "id": "NKOCPLAKPK_697", "locus": "GCA003260355_00661" }, { "type": "cds", "sequence": "contig_1", "start": 356062, "stop": 356271, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTCAAGCATCGCACTGAAACCCTAGCCATCATTAAATCCCTTTCTGAAATCATAAAAAGCACTAAAAAGCTGACCTTTTCGGTGAAATCCAGAGCAGGTTTGAATGAAGACGATAAGCCTGAACAGCTCCAGTTTCTTAGTCAGATTGCTCCTTTTTGTGATCTGATTACTATCCATGGGAGGACGCTCAAGCAACTTTATAGCTGA", "aa": "MLKHRTETLAIIKSLSEIIKSTKKLTFSVKSRAGLNEDDKPEQLQFLSQIAPFCDLITIHGRTLKQLYS", "aa_hexdigest": "2e058e98fbbea417f265d7083290c2c2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7874.141899999999, "isoelectric_point": 9.516967964172363 }, "id": "NKOCPLAKPK_698", "locus": "GCA003260355_00662" }, { "type": "cds", "sequence": "contig_1", "start": 356515, "stop": 356757, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTCACAACCTTACTCGGATTGATAATGAAACCATCAGCATTCATAAATTCAGTCCAAAGCTGCAATTCATCATCAGGATTTTTATACCTTTCAAAAATCTGACTGGTGATGAGGCGAGTCGCACAGTCGGTAATTCAATCCATAGGTGCAAAGCAGAGTTTCACTATTCCAAGAAGAAAAATAAAGCTGACTTAGTCTACGGAAAGACTCAGAAAAAAGCAAGAGAACTTTTGTCATAA", "aa": "MLHNLTRIDNETISIHKFSPKLQFIIRIFIPFKNLTGDEASRTVGNSIHRCKAEFHYSKKKNKADLVYGKTQKKARELLS", "aa_hexdigest": "443499ec0d5dd6eae2fba13d9d907a28", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9315.745399999994, "isoelectric_point": 10.06984882354736 }, "id": "NKOCPLAKPK_699", "locus": "GCA003260355_00663" }, { "type": "cds", "sequence": "contig_1", "start": 356867, "stop": 357202, "strand": "+", "frame": 2, "gene": null, "product": "Holliday junction resolvase-related domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EI90", "UniRef:UniRef90_A0A328EI90" ], "nt": "TTGGCATATTCTTTGGCAAATCTTGTTTTTCATTGTAAACTTTCTGCAATCAAGAAGAAGTCTATTTGTCATTCGAGAAGTTCTATTTTGGGAGAAGTGACAGAAAAAGTTGTCCCGCTTTTGCCAGAATTCCCTTATCATATGAAGGATTTAGTTTTTGTCGGAAAGGGGATTGATTATATCGTATTTGATGGGCTTTCGCGTGGGAAGTTGAGAGAAATTGTTTTTTTGGAAGTCAAAAGCAACTCCTCTCAGCTCAATAGAAATGAGCAAATGATTAAGGAGTGCTTGGGCGCTTTTTCTGTGAGGTATGAGGTAGTGAGGGTAAACTATTAG", "aa": "MAYSLANLVFHCKLSAIKKKSICHSRSSILGEVTEKVVPLLPEFPYHMKDLVFVGKGIDYIVFDGLSRGKLREIVFLEVKSNSSQLNRNEQMIKECLGAFSVRYEVVRVNY", "aa_hexdigest": "902eb69fe516e64ba49cc32af6dc1cd6", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A328EI90", "query_cov": 1.0, "subject_cov": 0.8740157480314961, "identity": 0.973, "score": 219.0, "evalue": 4.94e-72, "valid": true, "product": "Holliday junction resolvase-related domain-containing protein", "uniref50_id": "UniRef50_A0A328EI90" }, "pscc": { "uniref50_id": "UniRef50_A0A328EI90", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EI90" ], "product": "Holliday junction resolvase-related domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_700", "locus": "GCA003260355_00664" }, { "type": "cds", "sequence": "contig_1", "start": 357223, "stop": 357474, "strand": "-", "frame": 1, "gene": "dedD", "product": "Cell division protein DedD", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4IJ43", "UniRef:UniRef90_A0A328EGL2" ], "nt": "ATGAGAAATTGCTGTGCTGAACTTAACGCAATTGCAAACGCATCTCGTCAGGGCATCAGCCTAGAAGGAGCAACGCTCTATACCAAAATGACTCCTTGCTACGTGAGACATTGTGCTCATCTGATTATAAGTTGTGGAATCACAAGAGTTGTTTGCGAAAGGAAGTTCCACGATGCAAAGCTCAGCGAAGAAGCCTTCAAAAATGCAGGTGTAGAACTCACATATCTTGAGGATCAAGTTCAAAACTACTAG", "aa": "MRNCCAELNAIANASRQGISLEGATLYTKMTPCYVRHCAHLIISCGITRVVCERKFHDAKLSEEAFKNAGVELTYLEDQVQNY", "aa_hexdigest": "a47ed8d04d3e0e1a4658e1e269c3a069", "start_type": "ATG", "rbs_motif": null, "genes": [ "dedD" ], "truncated": "3-prime", "pseudogene": { "start": 357223, "stop": 357738, "observations": { "mutation": [ 357528 ], "stop": [ 357528 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EGL2", "query_cov": 1.0, "subject_cov": 0.4853801169590643, "identity": 0.988, "score": 171.0, "evalue": 3.53e-53, "gene_start": 1, "gene_end": 83, "reference_start": 89, "reference_end": 171, "reference_sequence": "MYQNGSIKDHGHLNPKDIAEDEYFMDICRVVGEKGTCDRGRSGCIIVKNGQVISTGFVDAPRGSKKCDEVGHQIWEVTKDTHETTKHCMRNCCAELNAIANASRQGISLEGATLYTKMTPCYVRHCAHLIISCGITRVVCERKFHDAKLSEEAFKNAGVELTYLDDQVQNY" }, "paralog": false, "identity": 0.9824561403508771, "score": 353.0, "evalue": 4.29e-127, "description": "Internal stop codon at 357528. Nonsense mutation around 357528.", "psc": { "uniref90_id": "UniRef90_A0A328EGL2", "product": "Cell division protein DedD", "uniref50_id": "UniRef50_A0A2W4IJ43", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EGL2", "UniRef:UniRef50_A0A2W4IJ43" ] }, "pscc": { "uniref50_id": "UniRef50_A0A2W4IJ43", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4IJ43" ], "product": "Cell division protein DedD" } }, "id": "NKOCPLAKPK_701", "locus": "GCA003260355_00665" }, { "type": "cds", "sequence": "contig_1", "start": 357526, "stop": 357738, "strand": "-", "frame": 1, "gene": "dedD", "product": "Cell division protein DedD", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4IJ43", "UniRef:UniRef90_A0A328EGL2" ], "nt": "ATGTATCAAAACGGAAGTATCAAAGACCATGGTCACCTTAATCCAAAAGACATCGCAGAAGACGAATATTTTATGGATATTTGCCGCGTAGTCGGAGAAAAAGGCACCTGTGATCGCGGAAGAAGCGGCTGCATTATTGTAAAAAATGGACAAGTAATCTCCACAGGCTTTGTAGATGCTCCTCGTGGAAGCAAAAAATGTGATGAAGTCTGA", "aa": "MYQNGSIKDHGHLNPKDIAEDEYFMDICRVVGEKGTCDRGRSGCIIVKNGQVISTGFVDAPRGSKKCDEV", "aa_hexdigest": "1f97f6875cb6c2fc08b3bc06623cd56b", "start_type": "ATG", "rbs_motif": null, "genes": [ "dedD" ], "truncated": "5-prime", "pseudogene": { "start": 357223, "stop": 357738, "observations": { "mutation": [ 357528 ], "stop": [ 357528 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EGL2", "query_cov": 1.0, "subject_cov": 0.4093567251461988, "identity": 1.0, "score": 154.0, "evalue": 2.03e-46, "gene_start": 1, "gene_end": 70, "reference_start": 1, "reference_end": 70, "reference_sequence": "MYQNGSIKDHGHLNPKDIAEDEYFMDICRVVGEKGTCDRGRSGCIIVKNGQVISTGFVDAPRGSKKCDEVGHQIWEVTKDTHETTKHCMRNCCAELNAIANASRQGISLEGATLYTKMTPCYVRHCAHLIISCGITRVVCERKFHDAKLSEEAFKNAGVELTYLDDQVQNY" }, "paralog": false, "identity": 0.9824561403508771, "score": 353.0, "evalue": 2.61e-127, "description": "Internal stop codon at 357528. Nonsense mutation around 357528.", "psc": { "uniref90_id": "UniRef90_A0A328EGL2", "product": "Cell division protein DedD", "uniref50_id": "UniRef50_A0A2W4IJ43", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EGL2", "UniRef:UniRef50_A0A2W4IJ43" ] }, "pscc": { "uniref50_id": "UniRef50_A0A2W4IJ43", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4IJ43" ], "product": "Cell division protein DedD" } }, "id": "NKOCPLAKPK_702", "locus": "GCA003260355_00666" }, { "type": "cds", "sequence": "contig_1", "start": 357790, "stop": 357972, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAACAGCCTATCGTTCTCGTAGCCAATAGCACCAATATTAGCATAGACCCAGTAGCAATCAGCCTTGTAAAAGATGGAAAGGTAGAAATAAAGCTCACAGCCAATCAAAATGGACCAGTATATACTGCAATCAATATGGGAACCAACAGAATGGGAGGTATAAGTATGCTCGTAATGTAA", "aa": "MKQPIVLVANSTNISIDPVAISLVKDGKVEIKLTANQNGPVYTAINMGTNRMGGISMLVM", "aa_hexdigest": "ccf2ee69b5d79a7332b0a0a46c5d3d3b", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6358.495300000002, "isoelectric_point": 9.398732948303223 }, "id": "NKOCPLAKPK_703", "locus": "GCA003260355_00667" }, { "type": "cds", "sequence": "contig_1", "start": 358231, "stop": 358917, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATTGTGCAGACTTCAAAAAAGGAATGAATGAAACGGATATCATCAATCAAGGACTTTGTAGGACCGAAATGTTCAATACTACCGTTGATCCAATTCCTCTGCTTGAATCTGCCAACGCAAAACTTCCTCATAAAGCCAATAATAATGAAAAAAGTGAAAAATCTGAACCAGAACACAATGCAGCTCCAGTAAATCCTGTAAATAAAGTTGATTTTGCAGATCGTGAACAAATTCTAAAAATTGCAAAACTCTATCTTGATAGTCCCTACCAAATGGGAGCAACCGGAACACTTCCTGGCAATCCAACCGACTGTAGCAAATTCACGCAAAATGTATTCACTAACGTTGGAATCGAGCTCGAAAGAAGTGCTGGAGATCAAGCTCATCAGTTTTCTAACGGAGGGTACCGATACGACAATCTAAACCAAGCTGAAATCGGCGACTTGCTCTTCTTCAAAAATACCTATCCTGCAGCCGTTGAGATCACACACGTTGGAATCTATGCCGGAAACGGACTTATGATCCACGCTGGAAATGAAAAGGTCGAGCTTACCACTATGGACGGCTACCGAAAAGAACATTTCAAGGGTATAGGATCCTTCAAATATCTGAGCATTAAATACAATAAAGAAAAAGCTACCCAAAGCTTCCTCTCGCTGAGTAAAATACCAAATAGTGCCTGA", "aa": "MNCADFKKGMNETDIINQGLCRTEMFNTTVDPIPLLESANAKLPHKANNNEKSEKSEPEHNAAPVNPVNKVDFADREQILKIAKLYLDSPYQMGATGTLPGNPTDCSKFTQNVFTNVGIELERSAGDQAHQFSNGGYRYDNLNQAEIGDLLFFKNTYPAAVEITHVGIYAGNGLMIHAGNEKVELTTMDGYRKEHFKGIGSFKYLSIKYNKEKATQSFLSLSKIPNSA", "aa_hexdigest": "731cd4605fab3106d0581fac8cd40746", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 25260.112800000003, "isoelectric_point": 6.082761192321777 }, "id": "NKOCPLAKPK_704", "locus": "GCA003260355_00668" }, { "type": "cds", "sequence": "contig_1", "start": 359269, "stop": 359676, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCAAGACTCATGAATAACCAATGGAAAAGATTAGAAGCATTTCAAGCTAAACTTCACAAAAGATTTGGGACGAGGATTCTCAAAAAACACCACAAAGGAAAAAGAATTGATAAAGCCTTCAATACTTCTAATTTTCTGCATCGTAACTGGATCTATATTGGAGAGCTTTCACTTGCGGTAGTGCTCAGTTGTTCTACCCTAATATTTGGAAACGGGAATGCTACTCCAAATAATCGTGAGCTTGTTTATCCTCTCACTGAGGTCTCTACCCTTGAGTGTAGAACAAAGGAATGGTCTACGTTAGCCTCTGATTGTAAAAAAGAACTTCCAATCATTAAAGGAGCAAACTATGCCAACTATGAGAATAATACGGATTATACTGATATTTATACCGTACTTTTTTGA", "aa": "MPRLMNNQWKRLEAFQAKLHKRFGTRILKKHHKGKRIDKAFNTSNFLHRNWIYIGELSLAVVLSCSTLIFGNGNATPNNRELVYPLTEVSTLECRTKEWSTLASDCKKELPIIKGANYANYENNTDYTDIYTVLF", "aa_hexdigest": "ed8c5312cb1ea00fc8b483f0f76dd170", "start_type": "ATG", "rbs_motif": "TTGTA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15698.867499999988, "isoelectric_point": 9.541465950012206 }, "id": "NKOCPLAKPK_705", "locus": "GCA003260355_00669" }, { "type": "cds", "sequence": "contig_1", "start": 359827, "stop": 360087, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTTGTATAAATCTAATTGTGCTGAATTTCTGTGGTCTTGTTGCTTGTCTATGGGCGCTTTTTGTTTATTTGGATTACTTTTTATCTAAAAAGGAGGCGCTTCATCTCCTTCAACCACTTGACTCACTTTTATGTTGGTTAGTTTTAATAGTGAAGGGATGGAGCTTGTTCGTGCTGGTTCTTGTTTGGTATTCAGATAATTATCCTTCTTATTTCACTTTCCCTTCCACTTCAAGTGGACTGTGCTTGCAATTCTGA", "aa": "MFCINLIVLNFCGLVACLWALFVYLDYFLSKKEALHLLQPLDSLLCWLVLIVKGWSLFVLVLVWYSDNYPSYFTFPSTSSGLCLQF", "aa_hexdigest": "ac1d50932022f20352779ac3282c09ea", "start_type": "ATG", "rbs_motif": "TTTTT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9967.852599999993, "isoelectric_point": 5.409276008605956 }, "id": "NKOCPLAKPK_706", "locus": "GCA003260355_00670" }, { "type": "cds", "sequence": "contig_1", "start": 360146, "stop": 360814, "strand": "+", "frame": 2, "gene": "hflX", "product": "GTPase HflX N-terminal domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EGN7" ], "nt": "ATGCAGGTAGAACAGCAGCCAGAACTTCGCGTATTTCTCGCAGATATTGTAGATAAACAGACTCCGAAAGCACTCCTTGAAGATAGGATGAAAGAGCTTGAAAACCTCGTGAATACCTATGGTGGGGTGGTGGTTTTGCAGAAATATCAGAAAAAAGATTTCCCAGACCTCAAAACTTATGTCGGGAAAGGGAAACTAGAAGAAATTATCGCAGATATGCTTCGCTTAAACGCAAATTTGCTGATCATTGGAAATGCACTTAAGCCCTCACAAATCTATCAAATCAATGAACAACTCCGCCTCGTGAGCGAGGAGCATAATCTCAAAGACAAAATGGTCGCTTGGGACAGGATTGATCTGATTTTGAAAATTTTTGAGAAACATGCAGAGGGAGGAGAAGCGAGATTGCAGATTGAGCTTGCGGCGATTAAGCATATGGGACCGAGGATTTATGGAATGGGAATGGAGCTGAGTAGGCAGGGAGGAAGCTCTGGAAGCGGAGGCGGAGCAACCAGAGGAATCGGAGAAACCAATACTGAAAGGATGAAAAGGCACTTGAAAGAGAAAGTAGGGAAAATAGAAAAAAAGCTGATTGAATACGAACAGATGAGAAAGCTCCATCGTGATGCTCGTAAAAAGAAGGGGATGCCTACGGTTGGGATCGTTTGA", "aa": "MQVEQQPELRVFLADIVDKQTPKALLEDRMKELENLVNTYGGVVVLQKYQKKDFPDLKTYVGKGKLEEIIADMLRLNANLLIIGNALKPSQIYQINEQLRLVSEEHNLKDKMVAWDRIDLILKIFEKHAEGGEARLQIELAAIKHMGPRIYGMGMELSRQGGSSGSGGGATRGIGETNTERMKRHLKEKVGKIEKKLIEYEQMRKLHRDARKKKGMPTVGIV", "aa_hexdigest": "c3037187ccda1664b0f5463b9bdc1085", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A328EGN7", "query_cov": 0.972972972972973, "subject_cov": 0.9, "identity": 0.583, "score": 219.0, "evalue": 1.51e-68, "valid": false, "product": "GTPase HflX N-terminal domain-containing protein", "uniref50_id": "UniRef50_A0A328EGN7" }, "pscc": { "uniref50_id": "UniRef50_A0A328EGN7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EGN7" ], "product": "GTPase HflX N-terminal domain-containing protein" }, "genes": [ "hflX" ], "id": "NKOCPLAKPK_707", "locus": "GCA003260355_00671" }, { "type": "cds", "sequence": "contig_1", "start": 360851, "stop": 360946, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACCAAAAAAGGCGTTTTAGCGGAAAATAAGCTCTTTGCAACGCTTGGAACGAATGTCGGGAAATGCTATTTGATTACCAATCCTGAAACCTGA", "aa": "MTKKGVLAENKLFATLGTNVGKCYLITNPET", "aa_hexdigest": "76f11c6c94302f3a8ce6831fe867855a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3355.9206000000017, "isoelectric_point": 9.03506679534912 }, "id": "NKOCPLAKPK_708", "locus": "GCA003260355_00672" }, { "type": "cds", "sequence": "contig_1", "start": 360962, "stop": 361306, "strand": "+", "frame": 2, "gene": null, "product": "Hflx-type G domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A9E5GTH0" ], "nt": "TTGCTCAATGATACGATCGGTTTCATCCGTGATCTCCCTCCAAAGCTGATTAAGTCCTTTTCTTCTACACTGGAGGACAGTATTGAGTCAGATTTGTTGCTTCATGTGATTGATGCGAGCGATCCTTTTGTAGAGGAGAGGATTGAGGTTGTGCACCAGGTTTTGTCTGATATTGGAGCGAATCAGGATAAGATTTTGGTGTTTAATAAGATTGATTTGATCAATGAAGAAAAAAAAGCTGAATTAGCTCAGAGATTCGCTGATTTTCCAGTGGTTTTTGTTTCGGTCAAAGAGGCGGTTGGCTTTGAGCAGTTGAGAGGAAGGTTAGTAGAGAAACTGAAGTAA", "aa": "MLNDTIGFIRDLPPKLIKSFSSTLEDSIESDLLLHVIDASDPFVEERIEVVHQVLSDIGANQDKILVFNKIDLINEEKKAELAQRFADFPVVFVSVKEAVGFEQLRGRLVEKLK", "aa_hexdigest": "cc762b5b6304ef3bf31cdb7ddd46d7c5", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A9E5GTH0", "query_cov": 0.8333333333333334, "subject_cov": 0.979381443298969, "identity": 0.632, "score": 124.0, "evalue": 9.59e-35, "valid": false, "product": "Hflx-type G domain-containing protein", "uniref50_id": "UniRef50_A0A9E5GTH0" }, "pscc": { "uniref50_id": "UniRef50_A0A9E5GTH0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A9E5GTH0" ], "product": "Hflx-type G domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_709", "locus": "GCA003260355_00673" }, { "type": "cds", "sequence": "contig_1", "start": 361460, "stop": 361750, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBF6A64", "UniRef:UniRef100_A0A328EKC8", "UniRef:UniRef50_A0A328EKC8", "UniRef:UniRef90_A0A328EKC8" ], "nt": "ATGTGGCGTAGAAAGTTCGTTCATAGCTTACATAAATTCAAGTCTCGGTTGCGGGTTCAGATGGAGAAACTTGATGCTATTCCTTATGTAATCAGATTGCCTCTCGGAGTGTTTTTTTTGATCATTGGGATCATTTTCTTTTATATCCCGTTTATTAACGGTCTCATCTTTATGCTTGTAGGAGCGAGAATGCTCGGTAAAAGGCATATGAATAGAGTTTTTCGCTGGGTTTCTTCTCAGAAATCTTATTCTTCTTCTCGTGAAGCACTTTTCAATCTTTTGAAAAAATAA", "aa": "MWRRKFVHSLHKFKSRLRVQMEKLDAIPYVIRLPLGVFFLIIGIIFFYIPFINGLIFMLVGARMLGKRHMNRVFRWVSSQKSYSSSREALFNLLKK", "aa_hexdigest": "ec4957df4be819bdd3293b0809c8d177", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000DBF6A64", "uniref100_id": "UniRef100_A0A328EKC8", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBF6A64", "UniRef:UniRef100_A0A328EKC8" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EKC8", "uniref90_id": "UniRef90_A0A328EKC8" }, "psc": { "uniref90_id": "UniRef90_A0A328EKC8", "uniref50_id": "UniRef50_A0A328EKC8" }, "pscc": { "uniref50_id": "UniRef50_A0A328EKC8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EKC8" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11534.888399999987, "isoelectric_point": 11.67788372039795 }, "id": "NKOCPLAKPK_710", "locus": "GCA003260355_00674" }, { "type": "cds", "sequence": "contig_1", "start": 361781, "stop": 361993, "strand": "+", "frame": 2, "gene": "hrcA", "product": "HrcA family transcriptional regulator", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A948C8S6" ], "nt": "ATGTCTGAAAATACTAAACTATTGGCTCTTCTGGATACCGTGATTGACTGTTATCTTGATAAAGGTGAGCCTGTCGGCTCTAAGTTTTTAAATTCTCTTGAGGCTGTAGATTATGCACCTTCTACCCTGAGGAAGTATTTAAATGCTTTGGAGAAGCAAGGGATGGTTTATCAGCCTTATAATTCTTCTGGAAGAATCCCGACATTGCAGTGA", "aa": "MSENTKLLALLDTVIDCYLDKGEPVGSKFLNSLEAVDYAPSTLRKYLNALEKQGMVYQPYNSSGRIPTLQ", "aa_hexdigest": "3fab5208bc361e0923ebd879a381c6c3", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A948C8S6", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.7859999999999999, "score": 112.0, "evalue": 4.03e-31, "valid": false, "product": "HrcA family transcriptional regulator", "uniref50_id": "UniRef50_A0A948C8S6", "kegg_orthology_id": "K03705" }, "pscc": { "uniref50_id": "UniRef50_A0A948C8S6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A948C8S6" ], "product": "HrcA family transcriptional regulator" }, "genes": [ "hrcA" ], "id": "NKOCPLAKPK_711", "locus": "GCA003260355_00675" }, { "type": "cds", "sequence": "contig_1", "start": 362018, "stop": 362449, "strand": "+", "frame": 2, "gene": "hrcA", "product": "Heat-inducible transcription repressor HrcA C-terminal domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A9E5L020" ], "nt": "TTGTCTGAGCGTCCAGATGATTTCGATTTGCCGCTTAATGATGCGAGGAAAGGCCTTAAAACCTTGGTGGAAGCTTTGGGGGAAGTCTCAGATGGGGTAGTAGTTGGTTTTATTAGGAATGATGAATATTATTACTTAGGAATTAATAATTTGCTTCGTGATGATATGATTGATGAGTATTCGGCTACGAGAACGATTGTTCGTTTTATTGAGGAGAAAAAGGTTGTTGCTTTTATTGACAAGAAAATACTGAAAAATCAGGAAGTTTATTATACGTTTGTGAATGATACAGATACTCTGATTTCTTGTGTTTATGTAAAGGTTAATTTGGAAGGGTATGATGCGGTGCTTTCTATTGTAGGGCCTCTGAGGGTGAATTATAAGAAAAATATTTCCATTCTCAAAAAAGTTTTAGAATCCTTAGCAAATTAG", "aa": "MSERPDDFDLPLNDARKGLKTLVEALGEVSDGVVVGFIRNDEYYYLGINNLLRDDMIDEYSATRTIVRFIEEKKVVAFIDKKILKNQEVYYTFVNDTDTLISCVYVKVNLEGYDAVLSIVGPLRVNYKKNISILKKVLESLAN", "aa_hexdigest": "2d3801b9ca384a5673ef0a1da2fb7c25", "start_type": "TTG", "rbs_motif": "TTATT", "psc": { "uniref90_id": "UniRef90_K1XW79", "query_cov": 0.8811188811188811, "subject_cov": 0.9545454545454546, "identity": 0.659, "score": 183.0, "evalue": 3.15e-57, "valid": false, "uniref50_id": "UniRef50_A0A9E5L020" }, "pscc": { "uniref50_id": "UniRef50_A0A9E5L020", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A9E5L020" ], "product": "Heat-inducible transcription repressor HrcA C-terminal domain-containing protein" }, "genes": [ "hrcA" ], "id": "NKOCPLAKPK_712", "locus": "GCA003260355_00676" }, { "type": "cds", "sequence": "contig_1", "start": 362451, "stop": 362939, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DC02040", "UniRef:UniRef100_A0A660MX02", "UniRef:UniRef50_A0A660MX02", "UniRef:UniRef90_A0A660MX02" ], "nt": "ATGAACAAGGAAAAAAGTCAGAATTTTGAAGCTGTATCCGCCTCGTCACTTGGAGAAAGACAGGAAAAACAGAAAGAACTCCAAGTTCGTATCAAAGAAATGCAGGAAAATTACTCTGCTGTGAAGGAAAAAATGCACAAAGAAGCTCGTGATTTGCAGGCTGATATTTTGTTGACTGACCTTGATAAGAGGATTGATCTCTTGGATGTGAAAAATAAGGTTGTTTTTGACTCAGAAGTGGAAAAAATTGAAGCGGAATTGGCAGTCTTTGATGAGGCTCATCGTTCCTTTTCTAACTTAAAAGGGAAAATTACCGCTCAGCATACCCAAGTTTATCAGCAGATGCAGAACCAATTCCCAAGCAGCTCAAATCAGGCAAACGAGGGTAGAGCAAAGAGCTATGAAGCGATTGCAGAGATTAAACCTCAGGACGGGGAGAATAAAGTTGCGAATTTTTTTGCTGGGATTGTGGAGAAACTGGTAAGTTAA", "aa": "MNKEKSQNFEAVSASSLGERQEKQKELQVRIKEMQENYSAVKEKMHKEARDLQADILLTDLDKRIDLLDVKNKVVFDSEVEKIEAELAVFDEAHRSFSNLKGKITAQHTQVYQQMQNQFPSSSNQANEGRAKSYEAIAEIKPQDGENKVANFFAGIVEKLVS", "aa_hexdigest": "de1583ae6fd985d6a25876fd8f82b399", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000DC02040", "uniref100_id": "UniRef100_A0A660MX02", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DC02040", "UniRef:UniRef100_A0A660MX02" ] }, "ips": { "uniref100_id": "UniRef100_A0A660MX02", "uniref90_id": "UniRef90_A0A660MX02" }, "psc": { "uniref90_id": "UniRef90_A0A660MX02", "uniref50_id": "UniRef50_A0A660MX02" }, "pscc": { "uniref50_id": "UniRef50_A0A660MX02", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MX02" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18462.494799999993, "isoelectric_point": 5.518236351013182 }, "id": "NKOCPLAKPK_713", "locus": "GCA003260355_00677" }, { "type": "cds", "sequence": "contig_1", "start": 363597, "stop": 364853, "strand": "+", "frame": 3, "gene": "eno", "product": "phosphopyruvate hydratase", "db_xrefs": [ "EC:4.2.1.11", "GO:0000015", "GO:0000287", "GO:0004634", "GO:0006096", "KEGG:K01689", "SO:0001217", "UniParc:UPI000DBFF815", "UniRef:UniRef100_A0A328EG04", "UniRef:UniRef50_Q11QE1", "UniRef:UniRef90_A0A2W4IK88" ], "nt": "ATGGCATATACTATTCAAAAAGTGCGAGCAAGAGAAGTGCTTGATTCTAGAGGAAATCCTACCGTTGAAGTAGAATTGACCCTCGCAAATGGCGTAATGCATAGAGCTTTAGTTCCTTCTGGAGCTTCTACAGGAGTGCATGAAGCTTTGGAGCTCAGAGATGGAGACAAGAGTAGATACCTTGGGAAAGGGACTTTGAAAGCGGTAGAAAATGTGAATACAACAATTGCAAAAGCAGTTGAAAATCAGACTTTTGATTGTTTTAGAGCTTTAGATAAAGCCTTAATTGAGCTTGATGGCACTCCAAACAAATCCAAGCTTGGAGCGAATGCAATCCTCGGAGTCAGTATGGCATTCGTAGGAGCCTGTGCAAAAGCGGAAGAAAAATGGATCTACGAATATCTCGGAGAAGGAAAAGGAACTACACTTCCTTACCCAATGATGAATATTCTCAATGGAGGTTCTCATGCGGATAATAATGTTGATATTCAGGAGTTTATGATTATTCCAAATGGAATGCCTAATTTCCACGAAGGACTCAGATGTGGAGCTGAAGTTTTCCACAACTTAAAAGCGATTTTGAAGGCAAAAGGGCTTGCGACTTCTGTAGGAGATGAGGGAGGATTCGCTCCAAATCTCGGTTCTAACGAGGAGGCGCTCCAAGTGATTGTTGAAGCAATTGAAAAAGCTGGATATACCGGAAAGGTTTCATTGGCATTGGATGTAGCTTCTAGCGAGTTTTACAAAGATGGAATGTATGATCTTGCAGGAGAGGGAAAAAAGCTGACTGCTGATGAACTTGTTGCTTTGTATCAGGATTGGTGTGAGAAATATCCGATCATCTCTATTGAAGATGGAATGGCTGAAGATGATTTTGAGGGATGGAAAAAGCTGACTGATGCTTTGGGAGAGAAGATTATGCTGGTAGGAGATGATCTTTTCGTAACCAATATCGCTAGACTTCAGATGGGAATTGATAAAGGAATTGCAAACTCAATCTTGATCAAACTTAACCAAATCGGATCAGTATCAGAAACCCTTGATGCTATCACTATGGCTCACGAAAACGGTATGAGATCAATTTCTTCTCACAGATCAGGAGAAACCGAAGATACCTTTATTGCTGATCTTGCTGTTGCGATGAATACCGGATTTATTAAGACCGGATCTGCTTCTAGAACTGATAGAATCGCGAAATATAACCAACTTTTGAGAATTGAAGAAAGACTTGGAGCGAGTGCGAAATATGGAAAATAA", "aa": "MAYTIQKVRAREVLDSRGNPTVEVELTLANGVMHRALVPSGASTGVHEALELRDGDKSRYLGKGTLKAVENVNTTIAKAVENQTFDCFRALDKALIELDGTPNKSKLGANAILGVSMAFVGACAKAEEKWIYEYLGEGKGTTLPYPMMNILNGGSHADNNVDIQEFMIIPNGMPNFHEGLRCGAEVFHNLKAILKAKGLATSVGDEGGFAPNLGSNEEALQVIVEAIEKAGYTGKVSLALDVASSEFYKDGMYDLAGEGKKLTADELVALYQDWCEKYPIISIEDGMAEDDFEGWKKLTDALGEKIMLVGDDLFVTNIARLQMGIDKGIANSILIKLNQIGSVSETLDAITMAHENGMRSISSHRSGETEDTFIADLAVAMNTGFIKTGSASRTDRIAKYNQLLRIEERLGASAKYGK", "aa_hexdigest": "b9dd60a85444c8acd3c6f7c96aeb6d62", "start_type": "ATG", "rbs_motif": "TTTTT", "ups": { "uniparc_id": "UPI000DBFF815", "uniref100_id": "UniRef100_A0A328EG04", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBFF815", "UniRef:UniRef100_A0A328EG04" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EG04", "uniref90_id": "UniRef90_A0A2W4IK88" }, "psc": { "uniref90_id": "UniRef90_A0A2W4IK88", "gene": "eno", "product": "phosphopyruvate hydratase", "ec_ids": [ "4.2.1.11" ], "uniref50_id": "UniRef50_Q11QE1", "kegg_orthology_id": "K01689", "go_ids": [ "GO:0000015", "GO:0000287", "GO:0004634", "GO:0006096" ] }, "pscc": { "uniref50_id": "UniRef50_Q11QE1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q11QE1" ], "product": "Enolase" }, "genes": [ "eno" ], "id": "NKOCPLAKPK_714", "locus": "GCA003260355_00678" }, { "type": "cds", "sequence": "contig_1", "start": 365103, "stop": 365687, "strand": "-", "frame": 2, "gene": "hflK", "product": "Modulator of FtsH protease HflK", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A088SZU7" ], "nt": "ATGACAGTAAGGATAGTAGACCAGTATGAAAGAAGAGTGGTACTCACTTTCGGAAAATTTACCAGAACACTCGGTGCAGGAATCAACTTCATCATCCCAATGGTAGAAAGGACGATCAATGTTGATATCAGAGAAAAAGCTGTCGATGTACCATCACAAGAGGCAATGACAAAGGACAATATCTCTTGTACTGTAAACGCGGTGATCTACTATAGAATCAAAGATGATCAAGTAGAAAGAGCCGTTCTCAACGTAAGAAACCTCGATTATGCTATGACACAGTTTGCCCTTACGACAATGAGAAATATCGTAGGACAATTTGAGCTGGATGAACTGCTTGCAAAGAGAGAAGAAGCCGCTAGTAAGATAAAAGAAATCGTTGATGAAAAATCTGATGCTTGGGGGGTTGATGTCCTCTCTGTAGAGCTCAAGGATATCAATATCCCTGACGACCTCAAAAGAACCATCGGAAAACAAGCAGAAGCAGAAAGAGAAAAAAGAGCAAAAATTATTACCTCCGAAGGAGAACTCGCAAGTGCGGAGAATCTCAAGAAAGCAGCAACAATGCTCGCCGAAGCACCATGA", "aa": "MTVRIVDQYERRVVLTFGKFTRTLGAGINFIIPMVERTINVDIREKAVDVPSQEAMTKDNISCTVNAVIYYRIKDDQVERAVLNVRNLDYAMTQFALTTMRNIVGQFELDELLAKREEAASKIKEIVDEKSDAWGVDVLSVELKDINIPDDLKRTIGKQAEAEREKRAKIITSEGELASAENLKKAATMLAEAP", "aa_hexdigest": "fa3e19ccbbc12fe8637794c98a46c6e3", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A0G0S0N6", "query_cov": 0.9329896907216495, "subject_cov": 0.8153153153153153, "identity": 0.552, "score": 192.0, "evalue": 8.64e-59, "valid": false, "product": "SPFH domain / Band 7 family protein", "uniref50_id": "UniRef50_A0A088SZU7" }, "pscc": { "uniref50_id": "UniRef50_A0A088SZU7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A088SZU7" ], "product": "Modulator of FtsH protease HflK" }, "genes": [ "hflK" ], "id": "NKOCPLAKPK_715", "locus": "GCA003260355_00679" }, { "type": "cds", "sequence": "contig_1", "start": 365885, "stop": 366040, "strand": "-", "frame": 3, "gene": null, "product": "Asp/Glu-ADT subunit C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EGM3", "UniRef:UniRef90_A0A328EGM3" ], "nt": "ATGCTTACTGAAAAAGAGATTGAGCATCTAGCAAAGCTTTCCAAACTTAACCTTACTCCGCAAGAAAAAGAAAGTTTCCAAAAAGGTCTCGACAATATTGTTGAATTTCTAGGACAGCTGAGCTCCATCGACACATCAAAAGCAAAAGAAACCTGA", "aa": "MLTEKEIEHLAKLSKLNLTPQEKESFQKGLDNIVEFLGQLSSIDTSKAKET", "aa_hexdigest": "eb8788e6b12118c7e018d2834252199e", "start_type": "ATG", "rbs_motif": "TTCTA", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 365762, "stop": 366040, "observations": { "mutation": [ 365887 ], "stop": [ 365887 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EGM3", "query_cov": 1.0, "subject_cov": 0.5543478260869565, "identity": 1.0, "score": 97.1, "evalue": 2.96e-25, "gene_start": 1, "gene_end": 51, "reference_start": 1, "reference_end": 51, "reference_sequence": "MLTEKEIEHLAKLSKLNLTPQEKESFQKGLDNIVEFLGQLSSIDTSKAKETGEESPMHCFTQEEDFENPKALLSNSKHQKGDFIAVKTSLSE" }, "paralog": false, "identity": 0.9782608695652174, "score": 176.0, "evalue": 7.82e-59, "description": "Internal stop codon at 365887. Nonsense mutation around 365887.", "psc": { "uniref90_id": "UniRef90_A0A328EGM3", "product": "Asp/Glu-ADT subunit C", "uniref50_id": "UniRef50_A0A328EGM3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EGM3", "UniRef:UniRef50_A0A328EGM3" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EGM3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EGM3" ], "product": "Asp/Glu-ADT subunit C" } }, "id": "NKOCPLAKPK_716", "locus": "GCA003260355_00680" }, { "type": "cds", "sequence": "contig_1", "start": 366259, "stop": 366357, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGGTAGTATGGTTTGGATCTTTGATGGTCTTTAGCTTGAGTACATTGCTCTCACCTCAGGTTTTTGCACAATCTGGAACTTCTTTACCTGGATGA", "aa": "MKVVWFGSLMVFSLSTLLSPQVFAQSGTSLPG", "aa_hexdigest": "a6af5c5fc9ca6bcf81673acc2d13564d", "start_type": "ATG", "rbs_motif": "TTATT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3416.0151000000005, "isoelectric_point": 8.500108146667483 }, "id": "NKOCPLAKPK_717", "locus": "GCA003260355_00681" }, { "type": "cds", "sequence": "contig_1", "start": 366388, "stop": 366684, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTCCAAGATTCTGCATTTAAGACCGCGGTTGATCAAGTGGCAGGATTGAGATTGAGTCTTTTTGACTTCAAAAAAAAGCTTGATCAAATGGATTCCAGTACTAAAGAACTTCACGCTGGAGAGTTTGATGCAGAATATAAGCTCATCAGGGCAGAAATGGTCAAAGTTATTCAAGATATTGATTATTCTACTGCTAAAATTACGAGAACGCTCAGGACGCTCTATACTCATAAACAAAATCTCCAGAAAACTCTCGATGAACTGCAAGAGACGAGAAAACATTTGGAAATCTGA", "aa": "MLQDSAFKTAVDQVAGLRLSLFDFKKKLDQMDSSTKELHAGEFDAEYKLIRAEMVKVIQDIDYSTAKITRTLRTLYTHKQNLQKTLDELQETRKHLEI", "aa_hexdigest": "e7e1d242e3fae5845a495c4ad9afb8b7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11432.981699999986, "isoelectric_point": 6.832069587707521 }, "id": "NKOCPLAKPK_718", "locus": "GCA003260355_00682" }, { "type": "cds", "sequence": "contig_1", "start": 366724, "stop": 367035, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGAGAGAGAAATTTATGACGAAGAGGGCGAACAAATAGATGCTGTTAAACTTTTTATCAAATCAGATGCTATGCCTCAGCTACTTGCTGGTGATGATACGCTCAAGATTTTGTTAAATCAGATTAATACTCTGATGAAGGAGGCAACCATGCAAGAAAAGCAGAAATCTGATACTATTGATAAGTTTGTTAAATTAAGAACTCAGGCTCAAAAATCACTTGATTATTACAAGACAGAGATTAAAAAACTTGAGCAGAAGAAGGCGTATTTGATGAGTTTTATGCAACTGTATAATGAAAAAAAACTTTGA", "aa": "MEREIYDEEGEQIDAVKLFIKSDAMPQLLAGDDTLKILLNQINTLMKEATMQEKQKSDTIDKFVKLRTQAQKSLDYYKTEIKKLEQKKAYLMSFMQLYNEKKL", "aa_hexdigest": "f3994656f9b24a5c964804aee22e590e", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12187.04849999999, "isoelectric_point": 6.320405006408691 }, "id": "NKOCPLAKPK_719", "locus": "GCA003260355_00683" }, { "type": "cds", "sequence": "contig_1", "start": 367045, "stop": 367374, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAGTGGAGTAAGTTCAGAGGAAGTGAATCTTCATGAAACTATTAATACTATGGTCGCTGATATCGTAGAAAAGAAATATCTGACGACAAATGATTTGCCAGAGAAAATAAAGCAATTGGCGGATCATACTGATAGTTCTGAAAATGAAACTTCTCCAGCTGCTTGGCCAACTTATCCAATTTCTCAGATTTTGACTATTTTTAAAGATCCGGCTTTTGAGAAGGAATATGGATTTAAAAATCTTTCTCTCAAACTTGCTGTAGAACAGGGAACACCGGTTTATGCGATGAGAGATGGTATTGTTTATTATACAGATACTGGTTCATGA", "aa": "MSGVSSEEVNLHETINTMVADIVEKKYLTTNDLPEKIKQLADHTDSSENETSPAAWPTYPISQILTIFKDPAFEKEYGFKNLSLKLAVEQGTPVYAMRDGIVYYTDTGS", "aa_hexdigest": "a9e34e523881d959922269b656e98faf", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12178.48149999999, "isoelectric_point": 4.563909721374513 }, "id": "NKOCPLAKPK_720", "locus": "GCA003260355_00684" }, { "type": "cds", "sequence": "contig_1", "start": 367390, "stop": 368148, "strand": "+", "frame": 1, "gene": null, "product": "Peptidase M23 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3D0URJ6" ], "nt": "ATGATTCTTCATACCGATGGTTATATCTCTACATATGCGTATTTGAGTAGGATTTTTGTTCAGCAAGGTGATTTTGTAAGAAGAGGTCAAATTATTGCACAGAGTGGAGGAGAACCTGGGACTGAAGGTGCTGGATTCGTTTCAAAAGGAGAAAATCTGACTTTTTCACTTTTTAAAGATGGAGTAGCGATTGATCCGCTTACGGTCCTTGATCTGAGTGTTGTTCAGGATAAAGATAAGGTTTTGCCTGAGGAATATCGCTTGAAATACTTTAATGATCAGTATGTTAGACCAATTGATATTACCAAGGTATCGCTCATAAAGGGGGATTCTGTCGATGAAAGGGCTCAGACTTTCCTTAATTCTTATGCAGTAGGTACGTATAGAAATGTTGCATTTTGGGATCAGGTCGTTGCTGGAACCAATATTGATAGAGATATGGTGATTTGTATCGGTTTTGCAGAGTCAACTCTTGGAAAATTTCTTGCTACGGATAATAATATCGGAAATGTCGGTAATAATGATAGAGGAGATCGTATTGCTTATAATAATCCGTTTAATGGAGCGAGGTTGATTCCTTTAACCTTGAATAATCAATATCTTGGAAATTATCATACTATTAAACAGCTTTCGAGATATGGAAATAGTGATGGCAAGATTTATGCCTCTAGTCCAATTAATTGGCAGAGTAATGTCCAGAAATGTCTCTCCAAGATTAAGGGATACTATGTTCCTGAGGATTTCCCTTTTAGAACTTGA", "aa": "MILHTDGYISTYAYLSRIFVQQGDFVRRGQIIAQSGGEPGTEGAGFVSKGENLTFSLFKDGVAIDPLTVLDLSVVQDKDKVLPEEYRLKYFNDQYVRPIDITKVSLIKGDSVDERAQTFLNSYAVGTYRNVAFWDQVVAGTNIDRDMVICIGFAESTLGKFLATDNNIGNVGNNDRGDRIAYNNPFNGARLIPLTLNNQYLGNYHTIKQLSRYGNSDGKIYASSPINWQSNVQKCLSKIKGYYVPEDFPFRT", "aa_hexdigest": "3026f84e6738d4df6106d0a41482eb33", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3D0URJ6", "query_cov": 0.996031746031746, "subject_cov": 0.8451178451178452, "identity": 0.665, "score": 354.0, "evalue": 1.14e-120, "valid": false, "product": "Peptidase M23 domain-containing protein", "uniref50_id": "UniRef50_A0A3D0URJ6" }, "pscc": { "uniref50_id": "UniRef50_A0A3D0URJ6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3D0URJ6" ], "product": "Peptidase M23 domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_721", "locus": "GCA003260355_00685" }, { "type": "cds", "sequence": "contig_1", "start": 368790, "stop": 369314, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACGGATATTTTTGCGGTTTTCCAGAACGTGGATAGGAACTCAATGCTCAAGATTGCTTTTGAGTATACTTTTAAGCTTGAGGACAGTGATTTGACGAAGCTCTTCTCTTTTATAGAGAAAGTGTTTAGCTATCTTTGGTGGGGAAGTCAGAAGAAAGAAGAGTCTGGTGAGACTAAAGAAGCTCCAAAGGATTTATTTATGAGCATTTGGTATAGTATTCAGACTCAGGATGCTTATATGAAGGAGAATCTGAATGCTCTTCTAAATTCTGTATTTGCACCTTTTCTCTCCAGCGGAAGTTTTGTAAAAAAAGAAAAAACTGAATGGATCCCGGCTAGTTTTTCTCAGGTGATCAATTTTTTTCATTTGCCGACCAAGATTAACTTTATCAAAGGGCTTGGTTATACGGTCTATAGAAAGTTGCCTTATCCTACTACGCTTCCTACTCCAGAAAATACGCCTGCAAAAGAGCTTACTCTCCTTGGGGATACTGATTACAGAGGTGATAAGATCAGATTTTGA", "aa": "MTDIFAVFQNVDRNSMLKIAFEYTFKLEDSDLTKLFSFIEKVFSYLWWGSQKKEESGETKEAPKDLFMSIWYSIQTQDAYMKENLNALLNSVFAPFLSSGSFVKKEKTEWIPASFSQVINFFHLPTKINFIKGLGYTVYRKLPYPTTLPTPENTPAKELTLLGDTDYRGDKIRF", "aa_hexdigest": "d1235f8aedb6943586c054208108fbe1", "start_type": "ATG", "rbs_motif": "TTCTT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 20252.966000000008, "isoelectric_point": 6.608465003967285 }, "id": "NKOCPLAKPK_722", "locus": "GCA003260355_00686" }, { "type": "cds", "sequence": "contig_1", "start": 369393, "stop": 370574, "strand": "+", "frame": 3, "gene": "traD", "product": "Type IV secretion system coupling protein TraD DNA-binding domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A922ZRB5" ], "nt": "ATGATTATCTCGGATATGAGGGCAGGAAATGGGCTTTGTCTGCTGGATCCACACGGAGAGCTCGTAGATGATTTGCTGGAATATATCCCATCAAACCGCATCAATGATGTGATTCTTTTTGATGTTGGAGATGCGGAATATCCGATCGGGTTCAACCTTTTGCAAGCCGATAATGAAGACGAAAAAAACAGAATTGCTTCTGGGGTTGTTTCCACTTTTCAGAAACTTTTTGACAATTCCCGAGGTCCAAGGCTAGAATATATTTTGAGAAATGTGGTTTTATCAGTAATTGACTATCCAAATGCAACCTTGATGCATATTCTGAGAGTCTTGACGGATAAAGAATTTCGTGAAGAGGTGATTTCCCATATCAAAGATTCAGTTTTATTAAAATTTTGGAATAACGAATTTAATAAATGGCAAGACAAACAGAGAGAAGAAGCCGTAGGACCGATTACCAACAAAGTCGGACAGTTTCTCTCTTCTCGCCTTGTGAGGAATATCTTTGGGCAGCCAAGAACGAAACTCAGTATCAGAAAAGCAATGGATGAGGGGAAGATCATTCTTGTGAACCTTTCAAAAGGAAAAATCGGTGAAGATAATGCTAATATGATTGGATCGCTCTTGGTAACGAAAATTCAGATTGATGCGATGGGAAGAGCCGATATGGCGAAACATTTGAGAAAGCCGTTTTACCTTTATATTGATGAGTTTCAGAACTTTGCGACCAAGTCCTTTGCGACGATTCTTTCAGAAGCGAGAAAATATAAGCTTTCTCTCATCGTAGCCAACCAATATACCTCTCAGCTTGATGAAGCGATCAAAGATGCGATTTTTGGAAATGTAGGGACGATTTTTTCCTTTACCCTTGGATATGATGATGCTTCTGTGATGACTTCGCAGTTTAAGGAAATGGTCAGCACTAATGACCTGATTTCTCTGCCAAAGTTTACCGCTTATACGAGGCTGATGGTAGATGGAATCTCTTCAGATCCTTTTAGTATGAAGACTTTGCCGCCTTTTGCATTGGAGGGGGATGTGGAGCATATTGAGAAGATTAGGAAGCAGTCTCGTCAGAGGTATGCAATGGAAAGAGAACAGTTAGAAAGTCTGATGAATGCTCGAAGTAAGAAATCTTTTTCAGCGCAAGAGAGGGTGGCAGAGCAGGCGAGGTATCAATGA", "aa": "MIISDMRAGNGLCLLDPHGELVDDLLEYIPSNRINDVILFDVGDAEYPIGFNLLQADNEDEKNRIASGVVSTFQKLFDNSRGPRLEYILRNVVLSVIDYPNATLMHILRVLTDKEFREEVISHIKDSVLLKFWNNEFNKWQDKQREEAVGPITNKVGQFLSSRLVRNIFGQPRTKLSIRKAMDEGKIILVNLSKGKIGEDNANMIGSLLVTKIQIDAMGRADMAKHLRKPFYLYIDEFQNFATKSFATILSEARKYKLSLIVANQYTSQLDEAIKDAIFGNVGTIFSFTLGYDDASVMTSQFKEMVSTNDLISLPKFTAYTRLMVDGISSDPFSMKTLPPFALEGDVEHIEKIRKQSRQRYAMEREQLESLMNARSKKSFSAQERVAEQARYQ", "aa_hexdigest": "cf17f003a51e4ac281beb49919d755f6", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A922ZRB5", "query_cov": 0.9389312977099237, "subject_cov": 0.8127753303964758, "identity": 0.534, "score": 409.0, "evalue": 9.47e-138, "valid": false, "product": "Type IV secretion system coupling protein TraD DNA-binding domain-containing protein", "uniref50_id": "UniRef50_A0A922ZRB5" }, "pscc": { "uniref50_id": "UniRef50_A0A922ZRB5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A922ZRB5" ], "product": "Type IV secretion system coupling protein TraD DNA-binding domain-containing protein" }, "genes": [ "traD" ], "id": "NKOCPLAKPK_723", "locus": "GCA003260355_00687" }, { "type": "cds", "sequence": "contig_1", "start": 370680, "stop": 371306, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00575.29" ], "nt": "ATGATTGTTGATACGGAATGGGGAAATCATAAAATGATTTGGTATCAACAGCCTGAAGGTATCGAGTCGATCGCGAGTTTACAGGTGCAAAAGGGTAAAATTGTAAAACTGGATGCAAATACTCAGCTTTCTTTCTTTGTGAGTATTTGGCAGCATAAAAGCCTGCTTTGCGCTAATAAAAAACCGCTTACGATTTGGGTCGGGACGAAGACTGATGTAGAACGCCAATTGAAGGAAATCTATCAGAAATCTGGTTTAGATGAAGAACATTCAGACTTTTTTAAGTTTGTGCCTAACGTTGATCTGATGACGGCTCAGCAGGAGCAGAAGAAAACCTCTCAAAAACCTTCCTTATCAAAAGCAGCGAAGCCTGCTCCGCGTAAGGCTCAGGCTTCTGTTGCTCTTTCACAAGCAGGGTTTTCTGTCCATGATGTTCAACTTGGGCAAGAATATGAAGGATATGTGAAGCTGATTTATAACTATGGGATGTTTATTACCGTTAAGGGGGTAGAAGGACTTCTTCATAAGAAGCAGATCGTCACGCCAGATGGAGTAGATTGGAAAAAATACTATAATATCGGGGATAAAATTAAGGTCAAAGCTTTTCAGTTTAAGGAGATTGACTGA", "aa": "MIVDTEWGNHKMIWYQQPEGIESIASLQVQKGKIVKLDANTQLSFFVSIWQHKSLLCANKKPLTIWVGTKTDVERQLKEIYQKSGLDEEHSDFFKFVPNVDLMTAQQEQKKTSQKPSLSKAAKPAPRKAQASVALSQAGFSVHDVQLGQEYEGYVKLIYNYGMFITVKGVEGLLHKKQIVTPDGVDWKKYYNIGDKIKVKAFQFKEID", "aa_hexdigest": "94c96a9547f7b6f705c770aa30af3ef0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00575.29", "name": "S1", "length": 56, "aa_cov": 0.2644230769230769, "hmm_cov": 1.0, "evalue": 1.4589306490858358e-08, "score": 31.436094284057617, "start": 146, "stop": 200 } ], "seq_stats": { "molecular_weight": 23755.077599999997, "isoelectric_point": 9.159619712829592 }, "id": "NKOCPLAKPK_724", "locus": "GCA003260355_00688" }, { "type": "cds", "sequence": "contig_1", "start": 371501, "stop": 371680, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EGF6", "UniRef:UniRef90_A0A328EGF6" ], "nt": "ATGTTGACGAAAAGGCAGATTTATCTTGTGATTTTTGCGGTGCTTTCTGTGCTTTTGGCGGTAGGGACGCTTTTTTATCTTAGGGAGCTTGATCGTAGGCTTCAGGAACCTCTCCAAGAGGAGTTTATCTATGAAGAAACGCCTGTAGTACCAGTTTCAGATTTATCATCTTCTTCATGA", "aa": "MLTKRQIYLVIFAVLSVLLAVGTLFYLRELDRRLQEPLQEEFIYEETPVVPVSDLSSSS", "aa_hexdigest": "a1ba2bf68a726e226aab892ddca23fb2", "start_type": "ATG", "rbs_motif": "TTTTT", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 371501, "stop": 371785, "observations": { "mutation": [ 371678 ], "stop": [ 371678 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EGF6", "query_cov": 1.0, "subject_cov": 0.6276595744680851, "identity": 0.983, "score": 106.0, "evalue": 6.83e-29, "gene_start": 1, "gene_end": 59, "reference_start": 1, "reference_end": 59, "reference_sequence": "MLTKRQIYLVIFAVLSVLLAVGTLFYLRELDRRLQEPLQEEFIYEETPVVPVSDSSSSSGADYLQEDFDALLSGETVADMYEGERDYGFTGSQN" }, "paralog": false, "identity": 0.9787234042553191, "score": 179.0, "evalue": 6.66e-60, "description": "Internal stop codon at 371678. Nonsense mutation around 371678.", "psc": { "uniref90_id": "UniRef90_A0A328EGF6", "uniref50_id": "UniRef50_A0A328EGF6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EGF6", "UniRef:UniRef50_A0A328EGF6" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EGF6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EGF6" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 6816.865399999999, "isoelectric_point": 4.601082420349121 }, "id": "NKOCPLAKPK_725", "locus": "GCA003260355_00689" }, { "type": "cds", "sequence": "contig_1", "start": 371789, "stop": 372403, "strand": "+", "frame": 2, "gene": null, "product": "SUF system FeS cluster assembly SufBD core domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MQ66", "UniRef:UniRef90_A0A328EKD6" ], "nt": "ATGGTAGCATATCATACTAATGCTGTATCGGAGCTTCTCCTCCAAGAAGATTCAGTTTTTGAACTTTGGGATGGAGCATGTGCTGAGAAGACGATCTTACTAGGAAATTCTAAACCACTCAAGTATGCTGCACTTGTGCATGCCAGCACGCTTGCTTTTCAGGTCAATACGATCGAGGCAGGAAGCGAACTTGAGATGATGCTTCTGTGTCCTGTGCTGGATACTCGTGAAACCTCGGTTAATCTTATGCTTAATCTCAGGCATAATGATTGTAAGATTCATCTCCATCTCGTAGCGCTTGTTTTTTCTGACGCGAAGACGACTTTGAATGCTACGATTTTTATGGAGCCACATATTCAAGGCGCGGCAAGTAAACTTTTGGAAGAAACGGTTGTGCTTTCTCCTGAGGTCCAGCTTCAGAGTCTTCCGATTCTTGATATTCAGGCAAAAAACATTCAAGCGGCTCATGGAGCGAAGATTTATCGTCTCGACCAAGATACGCTTTTCTATCTGCAGTCAAAGGGATTATCCTATCAGCAGGCACAATACCTCCTTCTTGCTTCCTATGGGGAAAGGCTTTTTGAAGGAGTCGCGTTGACGGAGGAGGAAAAATGA", "aa": "MVAYHTNAVSELLLQEDSVFELWDGACAEKTILLGNSKPLKYAALVHASTLAFQVNTIEAGSELEMMLLCPVLDTRETSVNLMLNLRHNDCKIHLHLVALVFSDAKTTLNATIFMEPHIQGAASKLLEETVVLSPEVQLQSLPILDIQAKNIQAAHGAKIYRLDQDTLFYLQSKGLSYQQAQYLLLASYGERLFEGVALTEEEK", "aa_hexdigest": "4f82b299529a62e3272e6b386aecf06e", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A328EKD6", "query_cov": 1.0, "subject_cov": 0.9444444444444444, "identity": 0.99, "score": 390.0, "evalue": 4.75e-137, "valid": true, "product": "SUF system FeS cluster assembly SufBD core domain-containing protein", "uniref50_id": "UniRef50_A0A660MQ66" }, "pscc": { "uniref50_id": "UniRef50_A0A660MQ66", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MQ66" ], "product": "SUF system FeS cluster assembly SufBD core domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_726", "locus": "GCA003260355_00690" }, { "type": "cds", "sequence": "contig_1", "start": 372445, "stop": 372717, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MVQ8", "UniRef:UniRef90_A0A660MVQ8" ], "nt": "ATGAAAGCATATTTTGATTTGGTATTGGATTTGCTTGAGATCGAGGAGAAGGAGCCCCTTTCTGCACTTGCAGAGGAGCTTGTGCTTGCTCATCAGCAAGGCAAACGCATCAAGATTGCTCATCGTCATCAGGTATTGTTTGAAGGGCGGCTTTTGCTCTTGGCGGGGAAACTTAGTCCTGAGGGGTTTGTGCAGATTGGTGATGTTGAGTCAGCTTTGCCTCTTTGGAAAGAAGAGGGGAGTCGTGAACTTCTCCAACAGCTACAATCCTGA", "aa": "MKAYFDLVLDLLEIEEKEPLSALAEELVLAHQQGKRIKIAHRHQVLFEGRLLLLAGKLSPEGFVQIGDVESALPLWKEEGSRELLQQLQS", "aa_hexdigest": "7784a8a1e714848f5a2d01ddc61f1e3f", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 372445, "stop": 372843, "observations": { "mutation": [ 372715 ], "stop": [ 372715 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A660MVQ8", "query_cov": 1.0, "subject_cov": 0.6818181818181818, "identity": 0.9329999999999999, "score": 156.0, "evalue": 1.21e-47, "gene_start": 1, "gene_end": 90, "reference_start": 1, "reference_end": 90, "reference_sequence": "MKAYFDLVLDLLEIEEKDPLSALAEELALAHQQGKRIKIAHRHQVLLEGWLLLLDGKLSPEEFVQIGDVESALPLWKEEGSRELLQQLQSGMLPEEELIIIDERAWKLFLSPDQQQQLLHLLEKENKAVIVK" }, "paralog": false, "identity": 0.9318181818181818, "score": 235.0, "evalue": 4.81e-81, "description": "Internal stop codon at 372715. Nonsense mutation around 372715.", "psc": { "uniref90_id": "UniRef90_A0A660MVQ8", "uniref50_id": "UniRef50_A0A660MVQ8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A660MVQ8", "UniRef:UniRef50_A0A660MVQ8" ] }, "pscc": { "uniref50_id": "UniRef50_A0A660MVQ8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MVQ8" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 10201.709899999998, "isoelectric_point": 5.1196813583374015 }, "id": "NKOCPLAKPK_727", "locus": "GCA003260355_00691" }, { "type": "cds", "sequence": "contig_1", "start": 372718, "stop": 372843, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTCCAGAGGAAGAACTGATTATTATTGATGAACGTGCATGGAAGCTTTTCCTTAGCCCGGACCAACAGCAAGAGCTTCTTGATCTCTTAGAAAAAGAAAATAAAGCAGTAATTGTAAAATAG", "aa": "MLPEEELIIIDERAWKLFLSPDQQQELLDLLEKENKAVIVK", "aa_hexdigest": "81765f004253809b3643ff4ae151bd91", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4878.637, "isoelectric_point": 4.356447410583496 }, "id": "NKOCPLAKPK_728", "locus": "GCA003260355_00692" }, { "type": "cds", "sequence": "contig_1", "start": 372909, "stop": 373190, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EJV3", "UniRef:UniRef90_A0A328EJV3" ], "nt": "ATGCCGAATAAAAATCTTCAAGCTTTCACGAAACTTTCTCAGAAGTATGTTCGTAGGTTTACTAATTATACCAAAAATCAAAAAGCTTTTTCACTCTTTTTGATGGTGTTTGCTTTTGCGCTGGTGGCTTTCCCGCTGGTGAAGATTGCTCCAGTCTTGATTTCGAAGAATGGGCAACCTACGATGGCAGATGCAAGTGATGCACAATCAATTTATCTCCTTGGGAAGACCTACTTTAAATCTATGTTGATCATTTTTTCGAGTCTGATTTTTTTGCTATGA", "aa": "MPNKNLQAFTKLSQKYVRRFTNYTKNQKAFSLFLMVFAFALVAFPLVKIAPVLISKNGQPTMADASDAQSIYLLGKTYFKSMLIIFSSLIFLL", "aa_hexdigest": "62e0b1213da4c040e12f473e838b3b6b", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 372909, "stop": 373493, "observations": { "mutation": [ 373188 ], "stop": [ 373188 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EJV3", "query_cov": 1.0, "subject_cov": 0.43457943925233644, "identity": 0.978, "score": 170.0, "evalue": 8.15e-52, "gene_start": 1, "gene_end": 93, "reference_start": 1, "reference_end": 93, "reference_sequence": "MPNKNLQAFTKLSQKYVRRFTNYTKNQKAFSLFLMVFAFALVAFPLVKIAPVLVSKNGQPTMADASDAQSIYLLGKTYFRSMLIIFSSLIFLLGWNMNIRFKGFIVNFLGFRESEPMLNFAFLWVIASVFMGILDTVRMLPEVTGQITLARGGNVMLILLLIGLVLSFIEVWKGASKNSQKTKILNIVDDEAPSKRGDGKRVLQHLFDDEDLEG" }, "paralog": false, "identity": 0.9690721649484536, "score": 367.0, "evalue": 6.71e-132, "description": "Internal stop codon at 373188. Nonsense mutation around 373188.", "psc": { "uniref90_id": "UniRef90_A0A328EJV3", "uniref50_id": "UniRef50_A0A328EJV3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EJV3", "UniRef:UniRef50_A0A328EJV3" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EJV3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EJV3" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 10564.537799999991, "isoelectric_point": 10.199817085266112 }, "id": "NKOCPLAKPK_729", "locus": "GCA003260355_00693" }, { "type": "cds", "sequence": "contig_1", "start": 373197, "stop": 373553, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4KQV0", "UniRef:UniRef50_A0A328EJV3", "UniRef:UniRef90_A0A328EJV3" ], "nt": "ATGAATATCAGGTTTAAGGGCTTTATCGTAAATTTCTTGGGATTTAGAGAGAGTGAACCGATGCTTAACTTTGCTTTCCTCTGGGTGATTGCAAGTGTCTTTATGGGAATCCTTGATACGCTTGGTATGCTTCCTGAGGTTACTGGTCAAATTACGCTGGCTCGAGGAGGAAAGGTGATGTTGATTCTGCTTTTGATTGGGTTGGTGCTCAGCTTTATAGAGGTGTGGAAAGGTGCGAGTAAGAACTCTCAAAAGACTAAAATTCTGAATATCGTCGATGATGAAGCTCCTTCCAAGAGAGGTGATGGCAAAAGGGTGCTTCAACATCTCTTTGATGATGAAGACTTGGAAGGTTAG", "aa": "MNIRFKGFIVNFLGFRESEPMLNFAFLWVIASVFMGILDTLGMLPEVTGQITLARGGKVMLILLLIGLVLSFIEVWKGASKNSQKTKILNIVDDEAPSKRGDGKRVLQHLFDDEDLEG", "aa_hexdigest": "d5984430a7c2b497a8b3f94e261d8371", "start_type": "ATG", "rbs_motif": "TTTTT", "psc": { "uniref90_id": "UniRef90_A0A2W4KQV0", "query_cov": 0.8220338983050848, "subject_cov": 1.0, "identity": 0.773, "score": 146.0, "evalue": 1.65e-43, "valid": false, "uniref50_id": "UniRef50_A0A2W4KQV0" }, "pscc": { "uniref50_id": "UniRef50_A0A2W4KQV0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4KQV0" ] }, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 372909, "stop": 373553, "observations": { "mutation": [ 373188 ], "stop": [ 373188 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EJV3", "query_cov": 1.0, "subject_cov": 0.5514018691588785, "identity": 0.975, "score": 224.0, "evalue": 1.21e-72, "gene_start": 1, "gene_end": 118, "reference_start": 97, "reference_end": 214, "reference_sequence": "MPNKNLQAFTKLSQKYVRRFTNYTKNQKAFSLFLMVFAFALVAFPLVKIAPVLVSKNGQPTMADASDAQSIYLLGKTYFRSMLIIFSSLIFLLGWNMNIRFKGFIVNFLGFRESEPMLNFAFLWVIASVFMGILDTVRMLPEVTGQITLARGGNVMLILLLIGLVLSFIEVWKGASKNSQKTKILNIVDDEAPSKRGDGKRVLQHLFDDEDLEG" }, "paralog": false, "identity": 0.9719626168224299, "score": 408.0, "evalue": 9.01e-148, "description": "Internal stop codon at 373188. Nonsense mutation around 373188.", "psc": { "uniref90_id": "UniRef90_A0A328EJV3", "uniref50_id": "UniRef50_A0A328EJV3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EJV3", "UniRef:UniRef50_A0A328EJV3" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EJV3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EJV3" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 13184.400199999984, "isoelectric_point": 5.781457710266112 }, "id": "NKOCPLAKPK_730", "locus": "GCA003260355_00694" }, { "type": "cds", "sequence": "contig_1", "start": 373583, "stop": 374452, "strand": "-", "frame": 3, "gene": null, "product": "Fungal lipase-like domain-containing protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DC0298C", "UniRef:UniRef100_A0A328EGD7", "UniRef:UniRef50_A0A328EGD7", "UniRef:UniRef90_A0A328EGD7" ], "nt": "ATGCAGATTAAGAAACGATTCATTGATCTCCCAGACCAACGAATACAGAAAAGCAAAAATTTCTTTTCTGATGCTTTTCCCAAAAATAAAGAGCTCAAATCAGTAGAAAAAAACCTGACTGAACTCTCTCCTACGACCAAAGATACCCTCACCACCCTTATCAAAAATAGTAAGATCAGCAAAAACATTGTCCTTCCTCTCGAGGACGAGCTTTGTGCACAGGTAACAAGATACGCATATTACGACCCAGAACAGAGACCCTATCAAATCGGCGACTTTTATCTCGAGCCCTACTACAACGATCTCTTTCATTGCATCTATCTCAACCATACCCAAAAAATCTGTATCATTGGATATAGGGGGACAGATACCAAAGAAAAATCTGATCTCCTTTCCGACGCTCAGATTATCCTCGGAGTAAATGCGATTGACCCCAGAGTAAGCGGAAGCCTCAAGCTCTTTGACCAAGTAAGAAAAAGCCATCCCGACTACCAAAAACGAATTTGCGGACATTCACTCGGAGGGACCCTCTGCTATATTGTAGCCAAACATAGAAGAGTTGATTATTGTTGCACCTTCAACCCCGGCTCCGCCCCCAACAAAATCTTCATCTTAATGCTCAAAGACACCCTCCTCAAAAAAGACCGAACCCAAAAGATCCACACCTACAAAATCATTGGTGATCCCATCTCACTTTGTAGCTATGTCGGCAAGACCGTCAGCTTTTTCGTGCCGCATTGGAACCCCCTCAAGCTCCATGCAATGGAAAATTTTCTCGGACAAGAAGCCTCAAAAAATACCGGAAACGCCTCCTCTACCGCGACCACTCCCGCTCCTCAATCCCCAGAAACAAAAAACCCTACCCCCTAG", "aa": "MQIKKRFIDLPDQRIQKSKNFFSDAFPKNKELKSVEKNLTELSPTTKDTLTTLIKNSKISKNIVLPLEDELCAQVTRYAYYDPEQRPYQIGDFYLEPYYNDLFHCIYLNHTQKICIIGYRGTDTKEKSDLLSDAQIILGVNAIDPRVSGSLKLFDQVRKSHPDYQKRICGHSLGGTLCYIVAKHRRVDYCCTFNPGSAPNKIFILMLKDTLLKKDRTQKIHTYKIIGDPISLCSYVGKTVSFFVPHWNPLKLHAMENFLGQEASKNTGNASSTATTPAPQSPETKNPTP", "aa_hexdigest": "9e8bb6a3de078896f5e9cb498713433c", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000DC0298C", "uniref100_id": "UniRef100_A0A328EGD7", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DC0298C", "UniRef:UniRef100_A0A328EGD7" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EGD7", "uniref90_id": "UniRef90_A0A328EGD7" }, "psc": { "uniref90_id": "UniRef90_A0A328EGD7", "product": "Fungal lipase-like domain-containing protein", "uniref50_id": "UniRef50_A0A328EGD7" }, "pscc": { "uniref50_id": "UniRef50_A0A328EGD7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EGD7" ], "product": "Fungal lipase-like domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_731", "locus": "GCA003260355_00695" }, { "type": "cds", "sequence": "contig_1", "start": 374442, "stop": 374738, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAGACCATATGGTGACTTATCTCAATCCCTACGACCTCAAGATCTTTACCCCCGCCCTCAAAGAGCAAATCAACAAAGTGCAAGAACTCGGCGTCACAAACATTGCAATCGATAGGACGACCATCTACCTCAAGCAAAACCTTATCAATGAAAGTGATACCAGTATCTTCGGACAGCTTTTTAGCGGGAAACCCACCATGGATGAAAAGAAAAAAGAAGAAGTTATGACAGCGATGATTGATCTCCTCAGCAACCAAAACCTTTTAGACCTCTTCAAGGAACATGCAGATTAA", "aa": "MQDHMVTYLNPYDLKIFTPALKEQINKVQELGVTNIAIDRTTIYLKQNLINESDTSIFGQLFSGKPTMDEKKKEEVMTAMIDLLSNQNLLDLFKEHAD", "aa_hexdigest": "6fc7e023539a90e95f6c6cda5af61b8d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11291.84319999999, "isoelectric_point": 4.895053672790527 }, "id": "NKOCPLAKPK_732", "locus": "GCA003260355_00696" }, { "type": "cds", "sequence": "contig_1", "start": 374847, "stop": 375290, "strand": "-", "frame": 2, "gene": null, "product": "DUF2207 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4KDM7", "UniRef:UniRef90_A0A660MWG6" ], "nt": "ATGACTATAATAGAACTACCTTTTATCCCTGCTTGCAAACGGATGGCGATCGATACGAATGCCCCGAAACTCGGACTCCAATACAGCACCAGCAATGACTTCTTCCCAGAAACCATTGAATTTTTGCAGAAGCTAGGGTTCATCAGTGTCTTGAGTATTGATATGGATATTAAATACTCCTTCCAAAAGACCAACCGATCCACAATCATCCAAGGAGCCGAGGCAGACATCTACGACAACGACCACGCAGTCAGTGATACTTTCTTTATCTACAAAATTACCATCCCAAGAGAGACCAGAATCACCCGTTCCCTCATTTCCATCAAGCTCAACCAAAAAAAATCCATCACCCTCAAAGAAGTTTTCGGCTTTATAAAATCTTTTCCTATGGTATCCCTCTTGATTTTTATGATACTCTCATTAATGATACCAGGAAAAATCTGA", "aa": "MTIIELPFIPACKRMAIDTNAPKLGLQYSTSNDFFPETIEFLQKLGFISVLSIDMDIKYSFQKTNRSTIIQGAEADIYDNDHAVSDTFFIYKITIPRETRITRSLISIKLNQKKSITLKEVFGFIKSFPMVSLLIFMILSLMIPGKI", "aa_hexdigest": "d3fc5b118b81d2eade5b3ae0ada485e2", "start_type": "ATG", "rbs_motif": "TTTTT", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 374544, "stop": 375248, "observations": { "mutation": [ 374849 ], "stop": [ 374849 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A660MWG6", "query_cov": 0.9047619047619048, "subject_cov": 0.4962686567164179, "identity": 0.992, "score": 253.0, "evalue": 9.02e-83, "gene_start": 15, "gene_end": 147, "reference_start": 1, "reference_end": 133, "reference_sequence": "MAIDTNAPKLGLQYSTSNDFFPETIEFLQKLGFISVLSIDMDIKYSFQKTNRSTIIQGAEADIYDNDHAVSDTFFIYKITIPRETRITRSLISIKLNQKKSITLKEVFGFIKSFPMVSLFIFMILSLMIPGKIGKIIQIVNAITLLIFILFFAYKFIRYFFDLTKRDSKSMQDHMVTYLNPYDLKIFTPALKEQINKVQELGVTNIAIDRTTIYLKQNLINESDTSIFGQLFSGKPTMDEKKKEEVMTAMIDLLSNQNLLDLFKEHAD" }, "paralog": false, "identity": 0.9914529914529915, "score": 449.0, "evalue": 9.9e-163, "description": "Internal stop codon at 374849. Nonsense mutation around 374849.", "psc": { "uniref90_id": "UniRef90_A0A660MWG6", "product": "DUF2207 domain-containing protein", "uniref50_id": "UniRef50_A0A2W4KDM7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A660MWG6", "UniRef:UniRef50_A0A2W4KDM7" ] }, "pscc": { "uniref50_id": "UniRef50_A0A2W4KDM7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4KDM7" ], "product": "DUF2207 domain-containing protein" } }, "id": "NKOCPLAKPK_733", "locus": "GCA003260355_00697" }, { "type": "cds", "sequence": "contig_1", "start": 375473, "stop": 375595, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EIA7", "UniRef:UniRef90_A0A328EIA7" ], "nt": "ATGACTATTAAAACAGCCGTAGATGCTTTTTCCCATGCTATCAACGAAGGATACAAAAGTGGGAAATTTCAGATGTGGATAGAAAAAGGGTTAGCGAATGAGAATGGAAAATTCCAAGATTGA", "aa": "MTIKTAVDAFSHAINEGYKSGKFQMWIEKGLANENGKFQD", "aa_hexdigest": "ac522744a2e305901e3241166f454291", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 375473, "stop": 375712, "observations": { "mutation": [ 375593 ], "stop": [ 375593 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EIA7", "query_cov": 1.0, "subject_cov": 0.5063291139240507, "identity": 1.0, "score": 85.9, "evalue": 3.39e-21, "gene_start": 1, "gene_end": 40, "reference_start": 1, "reference_end": 40, "reference_sequence": "MTIKTAVDAFSHAINEGYKSGKFQMWIEKGLANENGKFQDGALGRIIKEGTSEFKSAFARNLGRFNEVLDARRKQGSAP" }, "paralog": false, "identity": 0.9873417721518988, "score": 157.0, "evalue": 9.65e-52, "description": "Internal stop codon at 375593. Nonsense mutation around 375593.", "psc": { "uniref90_id": "UniRef90_A0A328EIA7", "uniref50_id": "UniRef50_A0A328EIA7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EIA7", "UniRef:UniRef50_A0A328EIA7" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EIA7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EIA7" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 4506.036200000001, "isoelectric_point": 6.536961555480957 }, "id": "NKOCPLAKPK_734", "locus": "GCA003260355_00698" }, { "type": "cds", "sequence": "contig_1", "start": 375669, "stop": 375827, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGTGCTCGATGCAAGGAGAAAGCAGGGGTCAGCTCCCTAAAAATCTTTCAGAGTTTTCTGATGCGATTAGTCAAACTAAGAAGAAGCTTTATAGCGAATATGATGCTTTAGCAAAAAGTGCTGGAGAGGGCGGAGCATTTGTAAGCACGGAATGA", "aa": "MKCSMQGESRGQLPKNLSEFSDAISQTKKKLYSEYDALAKSAGEGGAFVSTE", "aa_hexdigest": "6261b041ed917e7f08b78d3b4ebda07d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5605.2051, "isoelectric_point": 6.193199348449707 }, "id": "NKOCPLAKPK_735", "locus": "GCA003260355_00699" }, { "type": "cds", "sequence": "contig_1", "start": 376848, "stop": 376985, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATGAAATCTTTGATGATGTAAACGTAAGAAATGCCCTAAAAAATGCGGGGTATGATTATGTAAAATTCAATGATTTTGGAAAGGACTTTAAGCCACATACTACCTATTTTGCTACTGATGTAGAAAGTCGTTGA", "aa": "MYEIFDDVNVRNALKNAGYDYVKFNDFGKDFKPHTTYFATDVESR", "aa_hexdigest": "4007ff69d85060c94b4632edc7a8bfaf", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5342.8155000000015, "isoelectric_point": 4.989179039001464 }, "id": "NKOCPLAKPK_736", "locus": "GCA003260355_00700" }, { "type": "cds", "sequence": "contig_1", "start": 377322, "stop": 377468, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCTAGTCCAAAAACGACTGGGAAATTAAATCGCCCAGCGATCATTTATAAGAAAAAAATGGGAAACAAGTACTACTATGTAGAAAGCGTCAATACAAAAGATGGGAAGTTAGAACCTCAATCAATGTATATCAACAAACCGTAA", "aa": "MPSPKTTGKLNRPAIIYKKKMGNKYYYVESVNTKDGKLEPQSMYINKP", "aa_hexdigest": "ff7411ac4d0201292aa787162ea07f1a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5551.4621000000025, "isoelectric_point": 9.874316215515137 }, "id": "NKOCPLAKPK_737", "locus": "GCA003260355_00701" }, { "type": "cds", "sequence": "contig_1", "start": 377716, "stop": 377829, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAAAGACAACAGTAAATTATAGGTCTGCTGTTACAGGAAAGTATGTGAAAGAAAGCTATGCTAAATCACATCCTAAGACTACTGTAAGAGAGACAAATAAAAGTAAATAA", "aa": "MAKTTVNYRSAVTGKYVKESYAKSHPKTTVRETNKSK", "aa_hexdigest": "5d3347c2cc266bbeb87d4c235bf85fab", "start_type": "ATG", "rbs_motif": "TTTTT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4190.7377000000015, "isoelectric_point": 10.170935249328611 }, "id": "NKOCPLAKPK_738", "locus": "GCA003260355_00702" }, { "type": "cds", "sequence": "contig_1", "start": 378338, "stop": 378436, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EJT7", "UniRef:UniRef90_A0A328EJT7" ], "nt": "ATGACAAAAAACAAAAAGAAACCTACCTCATTACAGAAGAACCTCATGATTGGAATTACTTCTAGTTTAATGTTCTACATTATTGCTGAACTAGCATGA", "aa": "MTKNKKKPTSLQKNLMIGITSSLMFYIIAELA", "aa_hexdigest": "cdf6e2064f538f5a7a8e90a2d1617209", "start_type": "ATG", "rbs_motif": "TTTTTA", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 378218, "stop": 378436, "observations": { "mutation": [ 378268, 378340 ], "stop": [ 378268, 378340 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EJT7", "query_cov": 1.0, "subject_cov": 0.4444444444444444, "identity": 1.0, "score": 61.2, "evalue": 1.13e-11, "gene_start": 1, "gene_end": 32, "reference_start": 1, "reference_end": 32, "reference_sequence": "MTKNKKKPTSLQKNLMIGITSSLMFYIIAELAGLTEKFQDCIDRRILFIIKICIAVGIRYWMMGRIRNKFYD" }, "paralog": false, "identity": 0.9722222222222222, "score": 139.0, "evalue": 3.88e-45, "description": "Internal stop codon at 378268, 378340. Nonsense mutation around 378268, 378340.", "psc": { "uniref90_id": "UniRef90_A0A328EJT7", "uniref50_id": "UniRef50_A0A328EJT7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EJT7", "UniRef:UniRef50_A0A328EJT7" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EJT7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EJT7" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 3614.3869000000004, "isoelectric_point": 10.000287437438963 }, "id": "NKOCPLAKPK_739", "locus": "GCA003260355_00703" }, { "type": "cds", "sequence": "contig_1", "start": 378641, "stop": 379108, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EGG9", "UniRef:UniRef90_A0A328EGG9" ], "nt": "ATGCTGTATCTACTTACGATAAAAATGAGAATGATTAAAAAAATTGAAAAAAATATGTTTACTATTGCTTCTTTTTTGTTAGTTATTTTTTGGATTGTATTATTTTTTTTCTTCTCTAGTAAAAGTGTAAAGACCATTTCAACTGATTTTTGGATGACTAATTATCTTATTTATGAAGAACATAATCTTGTAGAACGATCCGATTTATTAAATATTTTATCAATTTTATTGGCAGCATATATTATCCCCAAAGCAATTCACCGCAATCAAAAAAAAGAAGATTTTACACAAAAACATTTAACTTCAATCTTAAATCAGTTTGAAGATAGTTTTCATGATTTAAATAAATTAGTATTTACAGAAATAACTATTAATGAAAAAGAAAACATTGAAACAACGATTTTATTAACCTCAAAAAGAATGGGATTTATTTTAGCTCAACTAGAACAAGCTAATAATCGTCTCTGA", "aa": "MLYLLTIKMRMIKKIEKNMFTIASFLLVIFWIVLFFFFSSKSVKTISTDFWMTNYLIYEEHNLVERSDLLNILSILLAAYIIPKAIHRNQKKEDFTQKHLTSILNQFEDSFHDLNKLVFTEITINEKENIETTILLTSKRMGFILAQLEQANNRL", "aa_hexdigest": "5d64da9a34b628dddb136b783a02155f", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 378461, "stop": 379108, "observations": { "mutation": [ 378571, 378580, 378640, 378643 ], "stop": [ 378571, 378580, 378640, 378643 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EGG9", "query_cov": 1.0, "subject_cov": 0.7209302325581395, "identity": 1.0, "score": 291.0, "evalue": 1.84e-98, "gene_start": 1, "gene_end": 155, "reference_start": 1, "reference_end": 155, "reference_sequence": "MLYLLTIKMRMIKKIEKNMFTIASFLLVIFWIVLFFFFSSKSVKTISTDFWMTNYLIYEEHNLVERSDLLNILSILLAAYIIPKAIHRNQKKEDFTQKHLTSILNQFEDSFHDLNKLVFTEITINEKENIETTILLTSKRMGFILAQLEQANNRLGGDAKKIETFKNHYIKFLNVFGKLGESNFSFDQEFKSEFEISFWRFQSEVYRLKESISVS" }, "paralog": false, "identity": 0.9813953488372092, "score": 408.0, "evalue": 3.78e-147, "description": "Internal stop codon at 378571, 378580, 378640, 378643. Nonsense mutation around 378571, 378580, 378640, 378643.", "psc": { "uniref90_id": "UniRef90_A0A328EGG9", "uniref50_id": "UniRef50_A0A328EGG9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EGG9", "UniRef:UniRef50_A0A328EGG9" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EGG9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EGG9" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 18430.5248, "isoelectric_point": 8.804463386535648 }, "id": "NKOCPLAKPK_740", "locus": "GCA003260355_00704" }, { "type": "cds", "sequence": "contig_1", "start": 379729, "stop": 379878, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTATTTGAAAGTAAGTCAAATATTCCCTTACTTTTCCCTAGATTAACTGAATGTATGGCTAATACAAAGGGACATACTACATGTAAGTGGTGGCTAACCTATTATAAAGATACAAAGTCTTCATATGTTTGTTTTTTTGATTTATGA", "aa": "MLFESKSNIPLLFPRLTECMANTKGHTTCKWWLTYYKDTKSSYVCFFDL", "aa_hexdigest": "4d4d32176dce25807615c5e353765e57", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5859.7915, "isoelectric_point": 8.53505001068115 }, "id": "NKOCPLAKPK_741", "locus": "GCA003260355_00705" }, { "type": "cds", "sequence": "contig_1", "start": 380113, "stop": 380430, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAGTTTCTCCGAAAAAAAGATTATATGATATGAAACAATTTAGATATAGGACTAGAAGACATAAAAAGATTAGGAAGAAACTGTTAATGAGATATAAAAAACGTCAAAAATATATCAAGGGAATCTCCTATTCAAATCGTTTCTCTAAAAAAGATCTTTTAGAAAAGAAAAAATTTAAATGTACTCCCCCTTCTTATTTCTCCTTTATTGAAAGTACTGATGAATTGATAGAATACTTCTATAGATATGAAAAAGCTCTTAGTCAAGGGAAACCCGTTAAGTTTGACTTAACTGATATTGATCAATTATGA", "aa": "MKKVSPKKRLYDMKQFRYRTRRHKKIRKKLLMRYKKRQKYIKGISYSNRFSKKDLLEKKKFKCTPPSYFSFIESTDELIEYFYRYEKALSQGKPVKFDLTDIDQL", "aa_hexdigest": "67ed364e22c456fb81821a08d3791e80", "start_type": "ATG", "rbs_motif": "TTATT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13065.323599999985, "isoelectric_point": 10.194079399108887 }, "id": "NKOCPLAKPK_742", "locus": "GCA003260355_00706" }, { "type": "cds", "sequence": "contig_1", "start": 380780, "stop": 380890, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAGAACTAGAAACCTTACTAAACTCTCTTATTAAGATGGTCTGGAAACCTAAAGACAGAGAGGTTATCTCAATTGCGCTTGATCCAGAATTTACAAAATCAGAATGA", "aa": "MQELETLLNSLIKMVWKPKDREVISIALDPEFTKSE", "aa_hexdigest": "e16b282d8d9e592ae518a520392f891d", "start_type": "ATG", "rbs_motif": "TTATT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4232.913299999999, "isoelectric_point": 4.889028739929199 }, "id": "NKOCPLAKPK_743", "locus": "GCA003260355_00707" }, { "type": "cds", "sequence": "contig_1", "start": 381297, "stop": 381539, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00521.26" ], "nt": "ATGGACGAAAAAGAACTTCTTCAGCCAAGTAATGAAAAGCTGATTACTCATCCGATAGAAAAAGAAATTACGCAGTCTTACATTGATTATGCGATGTCAGTGATTGTTTCTCGTGCCTTACCTGATACGAGAGATGGATTTAAGCCGGTGCTTAGGAGAATCCTCTTTGGAATGTATCAAATGAACAACTTCTACAACCAGAAGCACAAAAAATCAGCGAGAATTGTCGGAGATGTGATGTGA", "aa": "MDEKELLQPSNEKLITHPIEKEITQSYIDYAMSVIVSRALPDTRDGFKPVLRRILFGMYQMNNFYNQKHKKSARIVGDVM", "aa_hexdigest": "fba5a3c53f66fb597b16e6af3034a5ea", "start_type": "ATG", "rbs_motif": "TTTTT", "psc": { "uniref90_id": "UniRef90_UPI002A3F679F", "query_cov": 0.875, "subject_cov": 0.8641975308641975, "identity": 0.586, "score": 82.8, "evalue": 3.15e-19, "valid": false, "product": "DNA gyrase subunit A", "uniref50_id": "UniRef50_A0A352NSG9" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00521.26", "name": "DNA_topoisoIV", "length": 43, "aa_cov": 0.5375, "hmm_cov": 0.9767441860465116, "evalue": 4.085096188852387e-16, "score": 55.27061080932617, "start": 38, "stop": 80 } ], "seq_stats": { "molecular_weight": 9388.807299999997, "isoelectric_point": 9.003283882141115 }, "id": "NKOCPLAKPK_744", "locus": "GCA003260355_00708" }, { "type": "cds", "sequence": "contig_1", "start": 381579, "stop": 382085, "strand": "+", "frame": 3, "gene": null, "product": "DNA topoisomerase type IIA domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3D0UWC6" ], "nt": "ATGGTTCGTATGGCGCAACCATGGTCGCTCAGATATCCACTTGTTGATGGGCAGGGAAACTTCGGTTCTATTGATGGTGATGGAGCAGCTGCGATGAGGTATACTGAAGCGAGATTGACCAAGATTGCAGAGGAAATGCTTGCAGATTTGGAGCAGGATACGGTGGACTGGAGAGATAACTTTGATGGATCGCTCAAAGAGCCTGTGATGCTTGCGACCAAATTCCCAAATCATCTCTGCAATGGAACGATGGGAATTGCTGTAGGTATGGCGACGAATATGGCTCCGCATAACCTCAATGAAGTGTTGGATGCGTCTTTGCTTCTCCTTCAAAAGGAGGGGAAAGAGGAGATCGTGACTGATGAAAACGGTGCTGAGATCAAAAGACCGATTTCGGTTTCTGTTGAAGAGATTATGGAAATCATCAAAGGACCGGACTTTCCAACCGGAGGAATGATCTTTGATAGCAATAATATCACTGAAGTCTACAAAAAAGGAAAAGGCTGA", "aa": "MVRMAQPWSLRYPLVDGQGNFGSIDGDGAAAMRYTEARLTKIAEEMLADLEQDTVDWRDNFDGSLKEPVMLATKFPNHLCNGTMGIAVGMATNMAPHNLNEVLDASLLLLQKEGKEEIVTDENGAEIKRPISVSVEEIMEIIKGPDFPTGGMIFDSNNITEVYKKGKG", "aa_hexdigest": "60dcf3aff80bd2397a8c451ecc8b6ae5", "start_type": "ATG", "rbs_motif": "TTCTT", "psc": { "uniref90_id": "UniRef90_A0A3D0UWC6", "query_cov": 0.9940476190476191, "subject_cov": 1.0, "identity": 0.802, "score": 272.0, "evalue": 1.24e-91, "valid": false, "product": "DNA topoisomerase type IIA domain-containing protein", "uniref50_id": "UniRef50_A0A3D0UWC6" }, "pscc": { "uniref50_id": "UniRef50_A0A3D0UWC6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3D0UWC6" ], "product": "DNA topoisomerase type IIA domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_745", "locus": "GCA003260355_00709" }, { "type": "cds", "sequence": "contig_1", "start": 382134, "stop": 382613, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00521.26" ], "nt": "GTGATTGTCGTAGATGAGATTCCTTATCTCGTAAATAAAGCCAATCTCGTTTCCAAGATCGGAGAACTTGTGGTAGACAAGAAGATTGAGGGGATTAACGATATCCGTGATGAATCCAATAAAAACAAGATCAGAATTGCGATTTATCTCAAAAAAGGGGTAGATCCAGATGCGATTTTGATGCAGCTTTTCAAGTTCACCGAGCTTCAGACTAATTTCAATCTCAATAATGTGTCCCTGATTGATCAAGGAACTCAGCCGAGACTGCTCAATATCAAGGATCTCTTGATGGAGTTTGTGACTTTCCGTAGGGGGGTGGTGTATCGTAGATCGGTTTTTCAGCTCAATAAGGCTAAAGACAGACTACATATTTTGGAAGGACTGAGAAAAGCGATTGATATTATTGATGAAGTGATTGATACGATCAAAAAGTCAGAATCCAAAGCTGATGCGAGAACTAACTTAATGGAAAAATTTTGA", "aa": "MIVVDEIPYLVNKANLVSKIGELVVDKKIEGINDIRDESNKNKIRIAIYLKKGVDPDAILMQLFKFTELQTNFNLNNVSLIDQGTQPRLLNIKDLLMEFVTFRRGVVYRRSVFQLNKAKDRLHILEGLRKAIDIIDEVIDTIKKSESKADARTNLMEKF", "aa_hexdigest": "c3ce762ad209d6332c124eda01b81a11", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00521.26", "name": "DNA_topoisoIV", "length": 157, "aa_cov": 0.9874213836477987, "hmm_cov": 0.9872611464968153, "evalue": 4.810281324726607e-42, "score": 140.70944213867188, "start": 2, "stop": 158 } ], "seq_stats": { "molecular_weight": 18360.2768, "isoelectric_point": 9.244782447814941 }, "id": "NKOCPLAKPK_746", "locus": "GCA003260355_00710" }, { "type": "cds", "sequence": "contig_1", "start": 382647, "stop": 383123, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGATTGCAATCCCTCGTTGGACTTGAGATTCAGAAGATTAGTGATGAAATTGAGGAAAAGATTAGACTTATTGAGGAATTGGAGCGCATTATTTCTGATCCAGAGAAACTTGATGAGGTCGTAACTAAGGAATTCCTCTATATGAAGGAAAAATATGGAGACGAGAGAAGAACTGAACTTTCTAACGATCTCAGTGTCTATAACATCAGCGGAAGCCTCAAAGAATTCCAAAAAGCAGCGGATAGAATCAAGGAAGATGTGATTTGCTGGATTGGAAATGATTTTTCACTCAGAGTGCTGTATCAGACCAGAATTCAGAATATCCCTGAGGAAACCCTTGATCTCATCTATACTCACAACCAGGACAAACTCATCGTGATTACGGATATTGGAGAGCTTGTTGTGCAGAGGCTCAAAGATTTTGGTGCGGGAAATATGGCGAAACCTGCGATCAATCTCAAAGAACAATTCTGA", "aa": "MRLQSLVGLEIQKISDEIEEKIRLIEELERIISDPEKLDEVVTKEFLYMKEKYGDERRTELSNDLSVYNISGSLKEFQKAADRIKEDVICWIGNDFSLRVLYQTRIQNIPEETLDLIYTHNQDKLIVITDIGELVVQRLKDFGAGNMAKPAINLKEQF", "aa_hexdigest": "fe7f79b1f725b8907b50f4316d8418de", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18434.89679999999, "isoelectric_point": 4.774725532531738 }, "id": "NKOCPLAKPK_747", "locus": "GCA003260355_00711" }, { "type": "cds", "sequence": "contig_1", "start": 383346, "stop": 383795, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03989.18" ], "nt": "ATGCTCCTTTTCAAATCCGATGATATCAGACCGATGGGAAAGACTGCTGGAGGAGTGAAAGCGATTGAGCTTCAGGAAGGAGATCAGGTTGCCAATATGTTTTTGCATAATGATGAGCCATTTATCTTGATCTACTCTGATAAAAACGGAAAACTTCTCTCACTAGAAGACCTCAAAATCCGAAAGAGAGCGAGAAAGGGACAGGTCGTGATGACAGGCAATGAGAAACTTGAAGGTGGAATCTCAATCATTGAAGGTGCGGTCAGAATTCGTTTTGCTGATGGTAGCCTCAAAACTTTGCATTCCAACGATATTTCGCTTGATGAGCCAGAAACTCCGCTTTACAAGATGGTAGATCAGAAGATTGATGTCGTGTATCGTCCTTGGGAAGAGAAGTCTGAAAATATGAAGTATAAAGAAGAAAAGAAAAAGGCTGAAAAAGCTGAATGA", "aa": "MLLFKSDDIRPMGKTAGGVKAIELQEGDQVANMFLHNDEPFILIYSDKNGKLLSLEDLKIRKRARKGQVVMTGNEKLEGGISIIEGAVRIRFADGSLKTLHSNDISLDEPETPLYKMVDQKIDVVYRPWEEKSENMKYKEEKKKAEKAE", "aa_hexdigest": "f39cb38fd5b921a7ce90514eec4698ad", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03989.18", "name": "DNA_gyraseA_C", "length": 34, "aa_cov": 0.22818791946308725, "hmm_cov": 1.0, "evalue": 1.3094027738495124e-09, "score": 33.83357620239258, "start": 3, "stop": 36 } ], "seq_stats": { "molecular_weight": 16969.344699999994, "isoelectric_point": 6.201213645935058 }, "id": "NKOCPLAKPK_748", "locus": "GCA003260355_00712" }, { "type": "cds", "sequence": "contig_1", "start": 383973, "stop": 384104, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03013.19" ], "nt": "ATGACCAGAATCAACCTTTTGCCTCCTTCTCAACTCGCAGATCAGCACCTCATTGCCGAATGGAGGGAATTACCGAGGATTTTTGGGCTCGTAAAGAAAAAGCTTGATGAACAGAAGCCGATTCTCGTGTGA", "aa": "MTRINLLPPSQLADQHLIAEWRELPRIFGLVKKKLDEQKPILV", "aa_hexdigest": "80ae91da010e45eb51df226ea8845e3e", "start_type": "ATG", "rbs_motif": "TTTA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03013.19", "name": "Pyr_excise", "length": 37, "aa_cov": 0.7674418604651163, "hmm_cov": 0.972972972972973, "evalue": 1.2911522055374395e-08, "score": 31.59340476989746, "start": 1, "stop": 33 } ], "seq_stats": { "molecular_weight": 5048.9899000000005, "isoelectric_point": 9.519095420837402 }, "id": "NKOCPLAKPK_749", "locus": "GCA003260355_00713" }, { "type": "cds", "sequence": "contig_1", "start": 384117, "stop": 384368, "strand": "+", "frame": 3, "gene": null, "product": "Endonuclease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328ELA0", "UniRef:UniRef90_A0A328EG26" ], "nt": "ATGGGGACAGGGCATGTCCGTTTCTTTTATGATAAATTGCTTTTTCTTCAAAAGAGGCATCAAGCTTTAGTGAAAGAAGCTCAGAAAAGGGGATTTAAGCTCTCTAAGACTGAAAAAATTAGTTTACAGGGTTTCCCCAAAGTCTATTGTAAGGATTTTTGTCCTTCAGAGGCGGATCTGGCGATCTCCCAGCAAAGAATCCAAGAAAAACTCAAGGCAAAGCCAGATTTTTATACTTTTTATGGGAAGTAG", "aa": "MGTGHVRFFYDKLLFLQKRHQALVKEAQKRGFKLSKTEKISLQGFPKVYCKDFCPSEADLAISQQRIQEKLKAKPDFYTFYGK", "aa_hexdigest": "f093015773e6a0e84981caf86a75663a", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 383973, "stop": 384368, "observations": { "mutation": [ 384102 ], "stop": [ 384102 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EG26", "query_cov": 1.0, "subject_cov": 0.6014492753623188, "identity": 0.9279999999999999, "score": 158.0, "evalue": 1.89e-48, "gene_start": 1, "gene_end": 83, "reference_start": 49, "reference_end": 131, "reference_sequence": "MTRINLLPASELADQHLIAEWRELPRIFGLVKKKLDEQKPILVGKKYTMGTGHVRFFYDKLLFLQKRHQALVKEAVKRGFKLSKTEKISLQAFPKVYCQDFTPSQADLAISQQRIQEKLKAKSDFYTFYGKVRAFQED" }, "paralog": false, "identity": 0.9312977099236641, "score": 245.0, "evalue": 3.68e-85, "description": "Internal stop codon at 384102. Nonsense mutation around 384102.", "psc": { "uniref90_id": "UniRef90_A0A328EG26", "product": "Endonuclease", "uniref50_id": "UniRef50_A0A328ELA0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EG26", "UniRef:UniRef50_A0A328ELA0" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328ELA0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328ELA0" ], "product": "Endonuclease" } }, "id": "NKOCPLAKPK_750", "locus": "GCA003260355_00714" }, { "type": "cds", "sequence": "contig_1", "start": 384595, "stop": 384966, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02016.21" ], "nt": "ATGGGACTTGAGGTTGTTTTTATGCCGAATGCGCTTAATGGAATTGCCGATTTAGAGGAACATCCAGAAAAGAGAGCTAGTGATCTGAAACTTGCTTTCAAAGACACAACAATTTCTGGAATTATTTGTGCTATTGGTGGAATTGATGGATTTAAAATTTTTCCATATCTTATGGAGGATTCTGACTTTAAAGAGCTTGTCAGAACTTCTCCTAAAGTTTTTATGGGATTCTCGGATACGACTAATCATCATCTAATGTTTTATCGCTTAGGAATGCAAAGTTTCTATGGACCAGCTTTTTTGACTGATTTTGCAGAGATGGATCATCAGATGCTTCCCTATACTCAGCATCATTTGCAGATGCTTTTTTGA", "aa": "MGLEVVFMPNALNGIADLEEHPEKRASDLKLAFKDTTISGIICAIGGIDGFKIFPYLMEDSDFKELVRTSPKVFMGFSDTTNHHLMFYRLGMQSFYGPAFLTDFAEMDHQMLPYTQHHLQMLF", "aa_hexdigest": "011f88e3c4fb2370cb60a2a3d66af092", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02016.21", "name": "Peptidase_S66", "length": 98, "aa_cov": 0.7886178861788617, "hmm_cov": 0.9489795918367347, "evalue": 1.5733986926363621e-21, "score": 73.18790435791016, "start": 1, "stop": 97 } ], "seq_stats": { "molecular_weight": 14059.10179999999, "isoelectric_point": 5.162140083312986 }, "id": "NKOCPLAKPK_751", "locus": "GCA003260355_00715" }, { "type": "cds", "sequence": "contig_1", "start": 385147, "stop": 385551, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002209CC33", "PFAM:PF17676.7" ], "nt": "ATGCTGGTTGGAGGCGGATTTCCTGATCAAAAGTTGATCTATGATAAATATCAGATTTTCCCAACTTTAGAAGAACGAAAAGGAAAAATTCTTTTTATAGAAACTTCGGAAGAAAGGTCTACTCCTGCATTTTATGCACAGATGTTGAGGATTTTTAAGGAGAGGGGGATTTTTGATCAAATCAATGGAATTTTTGTAGGTAAGCCTCAAAATGAAATTTATTATCAAGAATATAAGCAGGTTTTATGTGAAATAGTTGATAATCCTGATTTGCCGATTCTTTATAATGTAAATTTTGGTCATGCATATCCTCGTTGCATTTTGCCTTATAATGCTTTGGTTGCTTATGATCATAATGAAAAGGTGCTTCGTTTCCTTGAGTCACCATTTCAAGAATCGCTTTAG", "aa": "MLVGGGFPDQKLIYDKYQIFPTLEERKGKILFIETSEERSTPAFYAQMLRIFKERGIFDQINGIFVGKPQNEIYYQEYKQVLCEIVDNPDLPILYNVNFGHAYPRCILPYNALVAYDHNEKVLRFLESPFQESL", "aa_hexdigest": "06dac472f89340da1dd10254f919496b", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00137A561F", "query_cov": 0.8731343283582089, "subject_cov": 0.823943661971831, "identity": 0.521, "score": 135.0, "evalue": 1.54e-38, "valid": false, "uniref50_id": "UniRef50_UPI002209CC33" }, "pscc": { "uniref50_id": "UniRef50_UPI002209CC33", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002209CC33" ] }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF17676.7", "name": "Peptidase_S66C", "length": 102, "aa_cov": 0.7089552238805971, "hmm_cov": 0.9607843137254902, "evalue": 1.2913532870519617e-20, "score": 70.6811294555664, "start": 20, "stop": 114 } ], "seq_stats": { "molecular_weight": 15750.893099999985, "isoelectric_point": 5.186126136779785 }, "id": "NKOCPLAKPK_752", "locus": "GCA003260355_00716" }, { "type": "cds", "sequence": "contig_1", "start": 385635, "stop": 385832, "strand": "+", "frame": 3, "gene": null, "product": "NERD domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EGP4", "UniRef:UniRef90_A0A328EGP4" ], "nt": "ATGACACAAGAACTCTTGCAAGTAAGCGACATCAAGTTTAAATTTGTGAATGTTTCTGAGGTAATGGAGGCAGAATTGAAGAAGGTGGTAGATCACCAAATTGCAAATAAGGGACAAACTATTCTTAGGAAGATTTATTCAAAGAATATTGATGCTAAGGTGATTATTGACTATACTATTATAAAGAATAAAAGCTGA", "aa": "MTQELLQVSDIKFKFVNVSEVMEAELKKVVDHQIANKGQTILRKIYSKNIDAKVIIDYTIIKNKS", "aa_hexdigest": "5776a68d4bb7f92ab46d8d3a1b6cdebf", "start_type": "ATG", "rbs_motif": "TTCTA", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 385635, "stop": 385985, "observations": { "mutation": [ 385830 ], "stop": [ 385830 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EGP4", "query_cov": 1.0, "subject_cov": 0.5603448275862069, "identity": 0.985, "score": 122.0, "evalue": 9.46e-35, "gene_start": 1, "gene_end": 65, "reference_start": 1, "reference_end": 65, "reference_sequence": "MTQELLQVSDIKFKFVNVSEVMEAELKKAVDHQIANKGQTILRKIYSKNIDAKVIIDYTIIKNKSGLYEADFIFTYNGERFVVENHKGFKVASDLVTHAFDKWKRKVLGFWGFLSK" }, "paralog": false, "identity": 0.9827586206896551, "score": 228.0, "evalue": 6.4e-79, "description": "Internal stop codon at 385830. Nonsense mutation around 385830.", "psc": { "uniref90_id": "UniRef90_A0A328EGP4", "product": "NERD domain-containing protein", "uniref50_id": "UniRef50_A0A328EGP4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EGP4", "UniRef:UniRef50_A0A328EGP4" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EGP4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EGP4" ], "product": "NERD domain-containing protein" } }, "id": "NKOCPLAKPK_753", "locus": "GCA003260355_00717" }, { "type": "cds", "sequence": "contig_1", "start": 385878, "stop": 385985, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGTTGAAAATCATAAGGGCTTTAAAGTTGCTTCAGATCTTGTTACTCATGCTTTTGATAAATGGAAGAGAAAAGTGCTCGGTTTTTGGGGATTTTTGAGTAAATAA", "aa": "MVENHKGFKVASDLVTHAFDKWKRKVLGFWGFLSK", "aa_hexdigest": "33062f754fc3a9fe629f16e000b6513b", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4107.7807999999995, "isoelectric_point": 9.997515296936037 }, "id": "NKOCPLAKPK_754", "locus": "GCA003260355_00718" }, { "type": "cds", "sequence": "contig_1", "start": 386051, "stop": 386533, "strand": "+", "frame": 2, "gene": "greA", "product": "Transcription elongation factor GreA", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBFB181", "UniRef:UniRef100_A0A328EJP4", "UniRef:UniRef50_A0A2M7XKE0", "UniRef:UniRef90_A0A660MW23" ], "nt": "ATGGCAAGTAAAAATTTTCTTACGAGAGAAGGATATGATAAGCTTATTCTTGAACTTCAAGAGCTAAAACAGGAGAAATTACCTGCGGTTTTGGAAAGACTTGCTGAGGCTAAGGCTATGGGAGATCTTTCTGAAAACTTTGAGTATAAATCTGCAATGGAGGATAAGGATTTTATCAACTCTAGGATTGTAGAAATACAGAATCTTATCAATGATGTTGAGATTATCGATAAGGATAAAGATTCATCTAAGTCTGATAAAGTTGTGGATTTTGGTTCGACTGTTACTTTTAAGATTGAAGATGATGATAAAGAATATCAGGTTAGAATCGTTGGAACAGGAGAAGTTGGGATAGAAGATGGAGTGATGATTTCTTTTGATTCTCCTATTGGACAGGCTATTAGAGGTAAAAAGGCTGGAGAAACTGTTAAAATGAGACATGAAAGCGGAAGAAAGAGTATCAAGATTTTGGCTATTTCTTAA", "aa": "MASKNFLTREGYDKLILELQELKQEKLPAVLERLAEAKAMGDLSENFEYKSAMEDKDFINSRIVEIQNLINDVEIIDKDKDSSKSDKVVDFGSTVTFKIEDDDKEYQVRIVGTGEVGIEDGVMISFDSPIGQAIRGKKAGETVKMRHESGRKSIKILAIS", "aa_hexdigest": "c53d5910baa6fff0a583ae7815383279", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000DBFB181", "uniref100_id": "UniRef100_A0A328EJP4", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBFB181", "UniRef:UniRef100_A0A328EJP4" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EJP4", "uniref90_id": "UniRef90_A0A660MW23" }, "psc": { "uniref90_id": "UniRef90_A0A660MW23", "product": "Transcription elongation factor GreA", "uniref50_id": "UniRef50_A0A2M7XKE0" }, "pscc": { "uniref50_id": "UniRef50_A0A2M7XKE0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2M7XKE0" ], "product": "Transcription elongation factor GreA" }, "genes": [ "greA" ], "id": "NKOCPLAKPK_755", "locus": "GCA003260355_00719" }, { "type": "cds", "sequence": "contig_1", "start": 386602, "stop": 386751, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATAATCCTTACGATTTCTATTTTAAAAAAGCGAAGCAGGAAGGATACAAGGCGAGAAGCGCTTTTAAGCTTGAAGAAATTCAAGAAAAGTTTTTTTTGATCAAAAAGACGACAAAGTATGTGCTTGATATTGGATGTGCTCCTTGA", "aa": "MYNPYDFYFKKAKQEGYKARSAFKLEEIQEKFFLIKKTTKYVLDIGCAP", "aa_hexdigest": "75e875b1c66f120cffd904b2b590f0fc", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5890.8237, "isoelectric_point": 9.301643562316894 }, "id": "NKOCPLAKPK_756", "locus": "GCA003260355_00720" }, { "type": "cds", "sequence": "contig_1", "start": 386794, "stop": 387126, "strand": "+", "frame": 1, "gene": null, "product": "Ribosomal RNA large subunit methyltransferase E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3D0UT82" ], "nt": "GTGGAAAATTATCAGGTGATTGGTTTAGATTTAAAGCCCGTTGAATTACATTTCCCTGGTTTGTTTACTTATGTGCAAGATATCTCACAAAAAGATCAGGTAAGAGCTCTTTTGCAGAGGCATCAGATTCCAAAATTTGATCTGATTCAATCTGATATGGCTCCGAATACTATTGGTTTAAAGGATATTGATGCGATGAGAGCAATAGATCTCTTGGAACAAACGTATTGGATCTATGATGAATTTTTGAGTGATACGGGTGTATTTGTGATAAAAGTCTTTATGGGACCTGGTTTTGAAGAATTTGTATTAAAGCTGAAGAAGAGGTTTTGA", "aa": "MENYQVIGLDLKPVELHFPGLFTYVQDISQKDQVRALLQRHQIPKFDLIQSDMAPNTIGLKDIDAMRAIDLLEQTYWIYDEFLSDTGVFVIKVFMGPGFEEFVLKLKKRF", "aa_hexdigest": "02b24fd86459a6ce2c5677cb163f180d", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3D0UT82", "query_cov": 0.8636363636363636, "subject_cov": 0.8878504672897196, "identity": 0.537, "score": 115.0, "evalue": 2.46e-31, "valid": false, "product": "Ribosomal RNA large subunit methyltransferase E", "uniref50_id": "UniRef50_A0A3D0UT82" }, "pscc": { "uniref50_id": "UniRef50_A0A3D0UT82", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3D0UT82" ], "product": "Ribosomal RNA large subunit methyltransferase E" }, "genes": [], "id": "NKOCPLAKPK_757", "locus": "GCA003260355_00721" }, { "type": "cds", "sequence": "contig_1", "start": 387395, "stop": 387592, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATGCTATAAAGAAACAGCTACGAGACAAACTTCAGGATGATATTAGTGAACTTAATGAGGGGCTTCAGGACTTGTTGGAGAAAGGTGTAAATAAGGGAACAATCGAGGAAGAAGATGTGATTTCTGAGATTGATGACATGGAGGGGAACATTAAGCTGCTTGAAAAATTTTATGATTTAGCGGATAAGCTTTGA", "aa": "MDAIKKQLRDKLQDDISELNEGLQDLLEKGVNKGTIEEEDVISEIDDMEGNIKLLEKFYDLADKL", "aa_hexdigest": "7057789e7053766020909c65520cfd79", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7466.316899999996, "isoelectric_point": 4.208097648620606 }, "id": "NKOCPLAKPK_758", "locus": "GCA003260355_00722" }, { "type": "cds", "sequence": "contig_1", "start": 387602, "stop": 387919, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF04542.20" ], "nt": "TTGACGATAGAAGAGGTTCTTGAGCAAGAGCAGGAAAGTAGTAAAAAGGAATCAAAACTTGGAAGAGTGAGTCTCTATGGGAAGCCAGATTTGATGGCTGATGGGCAGCATAAGGATTTTATAAAGCTCTATTTTAATGATATTTCTAAAATTCCGCTTCTTACTTATGAAGAAGAAAAGGAAATAGCGAGGAAAATCAAAAAAGGAGATGAAGAGGCCAAAAAAAAACTGATTGAGTCTAATCTCAGACTTGTGATTTCTATTGCTAAGAGATTTTTCGGTTCTCGTCTGACTTTTTCAGATTTGATTCAAGAGTGA", "aa": "MTIEEVLEQEQESSKKESKLGRVSLYGKPDLMADGQHKDFIKLYFNDISKIPLLTYEEEKEIARKIKKGDEEAKKKLIESNLRLVISIAKRFFGSRLTFSDLIQE", "aa_hexdigest": "e53ab6b32b76e5a2d4105d93950c5bcc", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF04542.20", "name": "Sigma70_r2", "length": 29, "aa_cov": 0.2761904761904762, "hmm_cov": 1.0, "evalue": 1.561720281403424e-06, "score": 24.346559524536133, "start": 77, "stop": 105 } ], "seq_stats": { "molecular_weight": 12222.956699999982, "isoelectric_point": 6.627165031433106 }, "id": "NKOCPLAKPK_759", "locus": "GCA003260355_00723" }, { "type": "cds", "sequence": "contig_1", "start": 388031, "stop": 388342, "strand": "+", "frame": 2, "gene": null, "product": "RNA polymerase sigma factor RpoD/SigA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3D0USX5", "UniRef:UniRef90_A0A9E5GSZ3" ], "nt": "ATGAGTAAACATGTCAGGATTCCGGTGCATTTGATTGATGAAATGAACTCTTATAATAAGGCTTATCAAATTCTTTTTCAAAAACTTGGTCGTGAACCGACAAGTAAAGAGATCGGTCAAAAATTGGGATTCCCTATTAAAAAAATCAAGAAGCTTGAAGAGGTGATTTTTGGAAATGTTTCTCTTGATAGGGAAGTCGGTGATGAAGGAAGGGATACTTTGGCTGATCTTTTGGAAGATGGAAATACACTGAGACCAGATCAAATTGCCGAAAGGGCTGCGTTGAGAAATAATCTTGATGCAATTTTGTGA", "aa": "MSKHVRIPVHLIDEMNSYNKAYQILFQKLGREPTSKEIGQKLGFPIKKIKKLEEVIFGNVSLDREVGDEGRDTLADLLEDGNTLRPDQIAERAALRNNLDAIL", "aa_hexdigest": "a6b4c84e0165265f7490e3ea282edc36", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 387731, "stop": 388342, "observations": { "mutation": [ 387917 ], "stop": [ 387917 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A9E5GSZ3", "query_cov": 1.0, "subject_cov": 0.45982142857142855, "identity": 0.893, "score": 182.0, "evalue": 2.32e-56, "gene_start": 1, "gene_end": 103, "reference_start": 122, "reference_end": 224, "reference_sequence": "MELYESKHRNNDSQYKDFIKLYFNDISKIPLLNSEEERDIAKRIKKGDEAAKRILIESNLRLVISIAKRFFGSRLSFSDLIQEGNVGLIKAIEKFDPDKEFKFSTYATRWIKQSITKAIADMTKHVRIPVHLIDEINSYNKTYQMLFQKLGREPTSKEIGQKLGFPIKKIKKLEEVIFGNVSLDREVGDEGRDTLADLLEDSNTLRPDQFAEKNTLRDNLDMIL" }, "paralog": false, "identity": 0.8817733990147784, "score": 353.0, "evalue": 4.18e-126, "description": "Internal stop codon at 387917. Nonsense mutation around 387917.", "psc": { "uniref90_id": "UniRef90_A0A9E5GSZ3", "product": "RNA polymerase sigma factor RpoD/SigA", "uniref50_id": "UniRef50_A0A3D0USX5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A9E5GSZ3", "UniRef:UniRef50_A0A3D0USX5" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3D0USX5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3D0USX5" ], "product": "RNA polymerase sigma-70 domain-containing protein" } }, "id": "NKOCPLAKPK_760", "locus": "GCA003260355_00724" }, { "type": "cds", "sequence": "contig_1", "start": 388436, "stop": 388555, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGACAAGAGAAAGAGTAAGGCAGATTGAACAAAAGGTTATTCAGAAACTGAAAGAACATCAAGGACTCCAAAAGATGTTGGGAATTGAGGATGATATAGAAAAAATGAATAGTGAATGA", "aa": "MTRERVRQIEQKVIQKLKEHQGLQKMLGIEDDIEKMNSE", "aa_hexdigest": "e25bf4038fc8b4d21f6b6b90116c9ef5", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4710.4176, "isoelectric_point": 6.567768287658691 }, "id": "NKOCPLAKPK_761", "locus": "GCA003260355_00725" }, { "type": "cds", "sequence": "contig_1", "start": 388548, "stop": 388715, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAATGAGGAAAGAAGAAAAGGGGACGTAAACCTGCTCCAAAAGTGGAAAAAGATGATGACGATGATTATTATGATGCTTCGTATGATGACGATGATGATTTTGACTTGGATGTGGATTTTAGTGGAAGTGATGAAGATTAATATATTTTTTGTGATTGGGCTGTAA", "aa": "MNEERRKGDVNLLQKWKKMMTMIIMMLRMMTMMILTWMWILVEVMKINIFFVIGL", "aa_hexdigest": "a721bbebaac7739003c47fb172c1f717", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6812.616699999997, "isoelectric_point": 10.160878181457516 }, "id": "NKOCPLAKPK_762", "locus": "GCA003260355_00726" }, { "type": "cds", "sequence": "contig_1", "start": 388956, "stop": 389609, "strand": "+", "frame": 3, "gene": "rny", "product": "ribonuclease Y", "db_xrefs": [ "GO:0003723", "GO:0006402", "GO:0016020", "SO:0001217", "UniRef:UniRef50_A0A288GTZ4", "UniRef:UniRef90_A0A328EJQ6" ], "nt": "TTGATTATAACAGCAGCTGTTTCGCTGACGCTCGGAGGTTTTGCTGGGTTTTATGGGTATAAGAGGACGCTTAATGAAAAGCAGCTTCGCTTTAAGAAAAAAATGGAGAGGGTGAAGGAACTTGAGGAAGAGCTTCTCCAAGAGGCGAAGAAAAAGGCTGAAACTGTCTTAGAACAAGCTGAGGTAAAGGCTCAGAAAATTGAAGATCAGAGGCTAGCAAAGATGGAAGAGATCCAAAATAGGCTCCTTATTAGAGAGGAAAAATTGGATCAAAAATTAGAGAGACTTGAAGAAGAAAAAAGAAAAGTGCTTGATAAACAAAGAGAAGTTGATGAAATTATTGAGGAGCAGAATAAGAAGCTTTCTGAAATTGCACAAATTAAGTGGGAGGATGCTAAGGAGCTGATTTTTAAGAGAGTAGAGGAAGATAATCAGAAAGAGATTGTGAGATTTATTGAAAAATTTAAGACGATTAAATCAGAGGAAGCGCAGAAAGAGGCAGCTCAAATTCTCGCTCAGGCTATGCCAAAGCTTGCTGCAGATACTGTGAGTGAATTTGTTTCTAAAGTGGTGGATCTTCCGAGTGAGGAATTTAAAGGGAAGCTGATTGGGCGTGAGGGGAGAAATATTTCACTTTTTGAAAAGTTGACGTGA", "aa": "MIITAAVSLTLGGFAGFYGYKRTLNEKQLRFKKKMERVKELEEELLQEAKKKAETVLEQAEVKAQKIEDQRLAKMEEIQNRLLIREEKLDQKLERLEEEKRKVLDKQREVDEIIEEQNKKLSEIAQIKWEDAKELIFKRVEEDNQKEIVRFIEKFKTIKSEEAQKEAAQILAQAMPKLAADTVSEFVSKVVDLPSEEFKGKLIGREGRNISLFEKLT", "aa_hexdigest": "e7d5df2211f443900e713f3f6808e794", "start_type": "TTG", "rbs_motif": null, "genes": [ "rny" ], "truncated": "both", "pseudogene": { "start": 388935, "stop": 389912, "observations": { "mutation": [ 388953, 389607 ], "stop": [ 388953, 389607 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EJQ6", "query_cov": 1.0, "subject_cov": 0.43141153081510936, "identity": 0.995, "score": 234.0, "evalue": 1.38e-71, "gene_start": 1, "gene_end": 217, "reference_start": 8, "reference_end": 224, "reference_sequence": "MLTPETGLIITAAVSLTLGGFAGFYGYKRTLNEKQLRFKKKMERVKELEEELLQEAKKKAETVLEQAEVKAQKIEDQRLAKMEEIQNRLLIREEKLDQKLERLEEEKRKVLDKQREVDEIIEEQNKKLSEIAQIKWEDAKELIFKRVEEDNQKEIVRFIEKFKTIKSEEAQKEAAQILAQAMPKLAADTVSEFVSKVVDLPSEEFKGKLIGREGRNISLFEKLTGVELLIDDTPLSVRISSFDSEKRFIAGKTLETLIKDGRINPFYIEKVYNQVVADLEVTLMDKGKEALTVLNLPMMNPEIVRTIGQFFLRYSYGQNLWIHSLEVAKICENIAIELGLDPLVAKKAGLLHDVGKVIAGAGESHTKIGADMLRKWGLDPIVVNAAESHHYDVEMTSPHSRVVAAADAMSASRPGARFDTKDFFIEKMGELEKLILEVDGVEKVYIMQAGREIMVFVNPKMISDTQVEGLLETIGKKIEEQLDYPGIIRITALRETKLVQYLR" }, "paralog": false, "identity": 0.9938461538461538, "score": 451.0, "evalue": 8.49e-159, "description": "Internal stop codon at 388953, 389607. Nonsense mutation around 388953, 389607.", "psc": { "uniref90_id": "UniRef90_A0A328EJQ6", "gene": "rny", "product": "ribonuclease Y", "uniref50_id": "UniRef50_A0A288GTZ4", "go_ids": [ "GO:0003723", "GO:0006402", "GO:0016020" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EJQ6", "UniRef:UniRef50_A0A288GTZ4", "GO:0003723", "GO:0006402", "GO:0016020" ] }, "pscc": { "uniref50_id": "UniRef50_A0A288GTZ4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A288GTZ4" ], "product": "Ribonuclease Y" } }, "id": "NKOCPLAKPK_763", "locus": "GCA003260355_00727" }, { "type": "cds", "sequence": "contig_1", "start": 389619, "stop": 390221, "strand": "+", "frame": 3, "gene": null, "product": "HD domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A351Y7N0" ], "nt": "TTGATTGATGATACGCCATTGTCAGTCAGAATTTCATCTTTTGATAGTGAAAAAAGATTTATTGCTGGGAAAACCCTCGAAACTCTAATTAAAGATGGTAGGATAAATCCTTTTTATATCGAGAAGGTATATAATCAGGTAGTTGCTGATCTTGAGGTAACGTTGATGGATAAGGGAAAAGAAGCTCTTACGGTTTTGAATCTTCCAATGATGAATCCTGAGATTGTGAGGACTATTGGTCAGTTCTTTTTGAGATATAGTTATGGGCAGAATTTGTGGATACATTCTTTGGAGGTTGCTAAAATTTGTGAAAATATAGCGATAGAGCTTGGTTTAGATCCTTTGGTTGCTAAGAAGGCTGGATTGCTTCATGATGTCGGAAAGGTGATTGCTGGAGCTGGAGAATCTCATACTAAGATTGGAGCAGATATGCTTCGTAAGTGGGGGCTTGATCCTATCGTTGTCAACGCTGCAGAATCTCATCATTATGATGTAGAGATGACGAGTCCTCATTCTCGAGTTGTTGCTGCTGCAGATGCTATGTCTGCCTCTAGACCTGGTGCAAGATTTGACACTAAGGACTTTTTTATTGAAAAAATGTGA", "aa": "MIDDTPLSVRISSFDSEKRFIAGKTLETLIKDGRINPFYIEKVYNQVVADLEVTLMDKGKEALTVLNLPMMNPEIVRTIGQFFLRYSYGQNLWIHSLEVAKICENIAIELGLDPLVAKKAGLLHDVGKVIAGAGESHTKIGADMLRKWGLDPIVVNAAESHHYDVEMTSPHSRVVAAADAMSASRPGARFDTKDFFIEKM", "aa_hexdigest": "9d0f88f26b5e509b4eabc5ebd5e56af4", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A351Y7N0", "query_cov": 1.0, "subject_cov": 0.970873786407767, "identity": 0.7, "score": 293.0, "evalue": 1e-98, "valid": false, "product": "HD domain-containing protein", "uniref50_id": "UniRef50_A0A351Y7N0" }, "pscc": { "uniref50_id": "UniRef50_A0A351Y7N0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A351Y7N0" ], "product": "HD domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_764", "locus": "GCA003260355_00728" }, { "type": "cds", "sequence": "contig_1", "start": 390234, "stop": 390392, "strand": "+", "frame": 3, "gene": null, "product": "Phosphodiesterase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1V5ZNR7" ], "nt": "TTGATTCTTGAGGTTGATGGTGTAGAAAAGGTTTATATTATGCAGGCAGGTAGGGAGATTATGGTTTTTGTGAATCCAAAAATGATTTCAGATACTCAGGTTGAAGGACTCTTGGAGACTATTGGAAAAAAGATTGAAGAACAGCTTGATTATCCGTGA", "aa": "MILEVDGVEKVYIMQAGREIMVFVNPKMISDTQVEGLLETIGKKIEEQLDYP", "aa_hexdigest": "5f9b5e3bdc61883118c23da4563aa8a6", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A948C2N3", "query_cov": 1.0, "subject_cov": 0.8125, "identity": 0.6729999999999999, "score": 70.9, "evalue": 3.43e-15, "valid": false, "uniref50_id": "UniRef50_A0A1V5ZNR7" }, "pscc": { "uniref50_id": "UniRef50_A0A1V5ZNR7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1V5ZNR7" ], "product": "Phosphodiesterase" }, "genes": [], "id": "NKOCPLAKPK_765", "locus": "GCA003260355_00729" }, { "type": "cds", "sequence": "contig_1", "start": 390474, "stop": 390806, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAAGCTTTTCTGCAGACTACTTTATATTATCTTTACTAGTATGTATCCGTATTTTGAACTTTTTGGAACAAAGATTTATTCAACAGGTCTTGGGATTGTGCTTTCTTGTTTAGCTTTTTTGATAACAGCTTATTTCCTTTGTAAAAGATATAATCAAGAGTTCCTTAGGTTGTTTAATGGCTTGCCATGGTTGTTAGTTGCTGTTTATGTCGGGGGACTTTATCTTTCTTTTGTATTGGAGAATGGAAGGATTATTCCGACTTCCCTTGGCTTTTTAAGTCCTTATGGGTATCACTTTAGTTTCGTTGGAGTTATTCTTGCTTGTTTTTGA", "aa": "MKLFCRLLYIIFTSMYPYFELFGTKIYSTGLGIVLSCLAFLITAYFLCKRYNQEFLRLFNGLPWLLVAVYVGGLYLSFVLENGRIIPTSLGFLSPYGYHFSFVGVILACF", "aa_hexdigest": "9013d99a38ac21fa9cd022d727d55386", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12649.033999999989, "isoelectric_point": 8.962991142272951 }, "id": "NKOCPLAKPK_766", "locus": "GCA003260355_00730" }, { "type": "cds", "sequence": "contig_1", "start": 390822, "stop": 390956, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAACAGTTTAGAAGGAGTGAAACGAAGAAAGTCTGGCTTGATATTCTGTTTTTTTCTTTTGTGAATGGCATAATTGTCTTGGGAATTGGTTTATTGCTGGGGGATAATTTTGTAGGAAAACCGTATGATTGA", "aa": "MKQFRRSETKKVWLDILFFSFVNGIIVLGIGLLLGDNFVGKPYD", "aa_hexdigest": "981862ec9275aaf3d6ee576d1fd2020a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5075.964800000001, "isoelectric_point": 9.397443580627442 }, "id": "NKOCPLAKPK_767", "locus": "GCA003260355_00731" }, { "type": "cds", "sequence": "contig_1", "start": 390966, "stop": 391106, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAGAGCCCTTATTGCTGAGAGTAGTTTGGTAAAGTATGGAACTGTTTATCCTATTGGGCTTTTTTTGTCGCTCGGGGCGTTGATTCTTAATGTTGTTATTACGTTTTGGAAATTTATAGCAAAGAAGGCGGGACTTTGA", "aa": "MRALIAESSLVKYGTVYPIGLFLSLGALILNVVITFWKFIAKKAGL", "aa_hexdigest": "64632f0acfcc44376e36dab8b30e0ccd", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5027.1029, "isoelectric_point": 9.997450828552246 }, "id": "NKOCPLAKPK_768", "locus": "GCA003260355_00732" }, { "type": "cds", "sequence": "contig_1", "start": 391468, "stop": 392865, "strand": "+", "frame": 1, "gene": null, "product": "Baseplate protein J-like domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7W4HGS0", "UniRef:UniRef90_A0A328EGM7" ], "nt": "ATGAAGTTTTTTTTCCTCAAGACTGATAGTTTGTATAAGATTTTCAAAACTTTGGAGAAAATCCCTGCACAGAAGCCTGTTCAGATTTTTATTGATCCTGAACATCCTTTTTTTGAAAATCAACGATGGGGGAAACAGCTTTCTGACCTTGTTAAAGAAAGAAATCTGAATATTACTTTTCTGGCGGAGAAGGAGTCTAGTAGAAACTATTTTCGTCAGCTCGGTTTAAAGGTGTATTTTGAGGAAGAGAAGCCTATTATTAAGATTTTGAAAACGATTTCATTATTTCTTTTTGATATTAAGAGATTTCATCTTCATGCTTATGATAGGAAAAAATATCTTTTTTATCTTGTGTTTGTAGCTGAAATTTTAGCGGGGCTTGGAATCCTTTGGTTTCTCTTTCTGCTTATTCTTCCGAGTGCGAAAATAACGCTTAAAGTAGCCCAAAATACAGAAGATATTATTTATAACTTTCGTTATTATCCTGCAGGGGAGAGTGGCCATCTTGGTCTAATTAAACAGATCAGTATTCCTTATTATACAGGGAATTTGCAGTATGCATATCAGCTTTCTATTAGTACGGAAAATATCCAGCATATTATTAATCCTTCTGCTGGTTATGTGAAGGTATATAATAAGACATCGAACACTATCAATCTTCTAGCCAATACTAGATTTGTGACGAATAATGGACTCATTTTCCTTTCTAAGATTCCGGTTACGATTCCTGCTGGGCTAGCAGATAATCCTTCAGAGACGAGGATTACGCTTTATGCGAATGATGTTGATGAAGAAGGGCTGATGATGGGGGTTAGAGGGAATATTTCTAAAGGCACAAAATTGACGATAAAAAATATTAGAGATAGCTTTTATCTTGGGCAAATCTGGGCAGAAAGTATAGAAGCTTTCACAGGAGGGAGCACTACTTCTGTTGGAATGGTTTCTGAAAAAGATAAAGAGCTTCTTTCTGCTAAGATTAGAGATGGAGTTTATGCTGATAAATTAAATATTGTAACTAGAGAGTTTAATCTGCCAGGAGCCCTTGTTTTGATGTTTGATTCTTTGATTAAGACGACTTTTCATTCGCTTTCTGTAGATGGAAAGATCGGAGAAAAGGCAACCTCTTTGAGAGGGCATGCTGAGGTTTCTTTTGATTTTTTCTACTTGAAATGGGCAGATATTGTGCAGGCTTTTACTTCTTATGTAAAGGAAAGGCAAGCTGACAGTATTCAACTCATCAGTATTAATCCTAATTCCTTTGCACCTATTAACGATATTAAACACACCACAAGAGATGAGGTTTTCATTATCCCAACCAAAGCGACCATCTTGCAAGGCTATGATTTTGGAAAAGACGTGAAGGGGATTCTCCCTACTATCAAGAATACTGTTTCTTGA", "aa": "MKFFFLKTDSLYKIFKTLEKIPAQKPVQIFIDPEHPFFENQRWGKQLSDLVKERNLNITFLAEKESSRNYFRQLGLKVYFEEEKPIIKILKTISLFLFDIKRFHLHAYDRKKYLFYLVFVAEILAGLGILWFLFLLILPSAKITLKVAQNTEDIIYNFRYYPAGESGHLGLIKQISIPYYTGNLQYAYQLSISTENIQHIINPSAGYVKVYNKTSNTINLLANTRFVTNNGLIFLSKIPVTIPAGLADNPSETRITLYANDVDEEGLMMGVRGNISKGTKLTIKNIRDSFYLGQIWAESIEAFTGGSTTSVGMVSEKDKELLSAKIRDGVYADKLNIVTREFNLPGALVLMFDSLIKTTFHSLSVDGKIGEKATSLRGHAEVSFDFFYLKWADIVQAFTSYVKERQADSIQLISINPNSFAPINDIKHTTRDEVFIIPTKATILQGYDFGKDVKGILPTIKNTVS", "aa_hexdigest": "1fdc92eddd0d2333807c3f031dd06817", "start_type": "ATG", "rbs_motif": "TTATT", "psc": { "uniref90_id": "UniRef90_A0A328EGM7", "query_cov": 1.0, "subject_cov": 0.9117647058823529, "identity": 0.983, "score": 894.0, "evalue": 0.0, "valid": true, "product": "Baseplate protein J-like domain-containing protein", "uniref50_id": "UniRef50_A0A7W4HGS0" }, "pscc": { "uniref50_id": "UniRef50_A0A7W4HGS0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7W4HGS0" ], "product": "Baseplate protein J-like domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_769", "locus": "GCA003260355_00733" }, { "type": "cds", "sequence": "contig_1", "start": 392866, "stop": 393000, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACTGTAGAGGAAGCCAGGAAGTATATCCTCCAATACTCAGAAATTGCTAGTGTAAAGATAGATTTGGGATTCTTTCATTCAGAAAAAATTCCCGATGTGAGATCGAGAATTAAGATTAATGTGGAATTATAA", "aa": "MTVEEARKYILQYSEIASVKIDLGFFHSEKIPDVRSRIKINVEL", "aa_hexdigest": "2b45e51d6e5367f340cd5761776ee898", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5166.945299999999, "isoelectric_point": 6.54440746307373 }, "id": "NKOCPLAKPK_770", "locus": "GCA003260355_00734" }, { "type": "cds", "sequence": "contig_1", "start": 393012, "stop": 393515, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAATCCGAACCAAAGTCCGAGCTTTCAAATCTTATGCAAGCATATACAAATCTCTTTACTCTGAGCAAGGAAATCCTGACCTTACAAAACAAAATTGAAAAAGCAACCAATTCTGACTTTGAAATTCTTGGGAACAATACAGAAAAAAGTGTAATTCGCTATACCATTTCGCCACAGCTTGAGGAAAACGGAAAAGTCAACAGTCTCCTCCTCCACAAAAAGATAGAAGACCTCATCCATAACGAAGTAATAGAACACGAGCTCCTCTTTAAACCGGAAGAAGCCGACAAAAATCTCACCGTTAATATCAATGGAGCACTTCTTTACGAAGAAAACAAAGATCTTCAAGATCCGCTCAAAGCAACCCAAGTCCTAGAAAAAATCAACAAATTCAACTTCCTATTCGAACAAAAACACACAGAATTAGCAAACAAACGAGAGGAAATTGAAGCGGAAAAAGAAAAAATGAAAGCAGTCCGTGATATTTTCAGAAACTTCTAA", "aa": "MESEPKSELSNLMQAYTNLFTLSKEILTLQNKIEKATNSDFEILGNNTEKSVIRYTISPQLEENGKVNSLLLHKKIEDLIHNEVIEHELLFKPEEADKNLTVNINGALLYEENKDLQDPLKATQVLEKINKFNFLFEQKHTELANKREEIEAEKEKMKAVRDIFRNF", "aa_hexdigest": "6470004e4f027dac9c7c983a6dcf26e3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19490.916600000008, "isoelectric_point": 5.15946865081787 }, "id": "NKOCPLAKPK_771", "locus": "GCA003260355_00735" }, { "type": "cds", "sequence": "contig_1", "start": 393920, "stop": 394027, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAACTCAAACCCCAGCTACCACAGCAAATACAGTTTCTCAAAATCAAGAATCCTGAACCAAAAGATCGTTTTTCTGAGGCAACGAAAGTGTCTTTGAAAACCTAG", "aa": "MQLKPQLPQQIQFLKIKNPEPKDRFSEATKVSLKT", "aa_hexdigest": "c91688c254476e6d5874d0fe795c9a6e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4110.820100000001, "isoelectric_point": 9.997450828552246 }, "id": "NKOCPLAKPK_772", "locus": "GCA003260355_00736" }, { "type": "cds", "sequence": "contig_1", "start": 394039, "stop": 394284, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCTAAGAAGAATCTAAAACGATTTTTATTATATTCAGAAAGCTTTATAAAGCAAATTATTGTTCTTTTTTTGTCGTTTTTTGATAAAAAGATTTGTATTATGAAGACTAAATCATATACTCTGGTTATCGCTCTTAGGCGATTCTTTAATTTATTATTCTTTCGGTTAAATGACAGAAGAAATGGTAATCAGCCAGACAAAATTATTCGTGGGTTGATTGAACTGGAAAATGAGGGGAAATGA", "aa": "MPKKNLKRFLLYSESFIKQIIVLFLSFFDKKICIMKTKSYTLVIALRRFFNLLFFRLNDRRNGNQPDKIIRGLIELENEGK", "aa_hexdigest": "8e621558063b340db63592325498bc96", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9773.62669999999, "isoelectric_point": 10.287494087219237 }, "id": "NKOCPLAKPK_773", "locus": "GCA003260355_00737" }, { "type": "cds", "sequence": "contig_1", "start": 394271, "stop": 394567, "strand": "+", "frame": 2, "gene": null, "product": "RNA-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4KUX4", "UniRef:UniRef90_A0A660MUE4" ], "nt": "ATGAGGGGAAATGACCTCAAAACGGAATTCTCTAAATTTGGAGAAGTTGTATCAGCAAGAGTGAATCTTGATCAAACAACAAAACCTCCAAGGAGTAGAGGATTTGGATTCGTAGAATTTTCAAATGCTGCGGATGCTGCTAAAGCTCAAGCTGAGATGGATGGTGTAGAGGTTTGGGGGAGAATGATTAAGGTCGATTTTGCTAAAGAAAACCCTAACAGAGTCCCTAGATCTGAATATCAACATGAATCAAAGGTAAATGCTTTTATGGGAGATGATGATGACGTTACTTTCTAG", "aa": "MRGNDLKTEFSKFGEVVSARVNLDQTTKPPRSRGFGFVEFSNAADAAKAQAEMDGVEVWGRMIKVDFAKENPNRVPRSEYQHESKVNAFMGDDDDVTF", "aa_hexdigest": "10018cbc69f89573a3f13c8a1adfb30f", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A660MUE4", "query_cov": 1.0, "subject_cov": 0.8305084745762712, "identity": 1.0, "score": 205.0, "evalue": 6.65e-67, "valid": true, "product": "RNA-binding protein", "uniref50_id": "UniRef50_A0A2W4KUX4" }, "pscc": { "uniref50_id": "UniRef50_A0A2W4KUX4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4KUX4" ], "product": "RRM domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_774", "locus": "GCA003260355_00738" }, { "type": "cds", "sequence": "contig_1", "start": 395016, "stop": 395621, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAGGAAAGGGAGAAAAAACCTTTACCTGCGAAGCAATGCTCGACCTCAGCAAACCAACGAAACTCAAATTCCTTGATCTCAAAGTCAAAGAAACCGCTCAAACAGCAGAAGCAACCGAAACTGGTTCCAAAAATAACGAAACAACCACCAGCAAGAGCGAAACAAAAGTCGAGCCAAAAAAGGAAGAGCCAACAAATCCCTCCAAGGAAACTTCAAACGCAAATAAACTCCCAACAACCCCTAATGTAAAACAGTTTCCTGTTAACCTTGAAAAGACAATGAGCTTAAGTTCAAGCAGAGGAGAATATACGATAGTCTTTCCATCTCCAAATATTGCATATTCTATAGATAGTGCAGACGAAGATTTTGGACAGGCAGGGGTTCGCTGTAATTATGCAACCAGAGTGATCCAATATACCGACGAGGAAAATCTGCAGTCCTCTCCAAGCGTGATCATCTACGAATGTAGTTTCAAAAAAGACTTTACCCTTCCTGGTGATAATTACCTCCTCAAAGAGTTTGGCAATAAAAAATTCGTTATCGAAGTCAAAAATCCAGCCCGATTTGACTTTGCAAAACAAATCTCGATCGAAAAACTCTAA", "aa": "MEGKGEKTFTCEAMLDLSKPTKLKFLDLKVKETAQTAEATETGSKNNETTTSKSETKVEPKKEEPTNPSKETSNANKLPTTPNVKQFPVNLEKTMSLSSSRGEYTIVFPSPNIAYSIDSADEDFGQAGVRCNYATRVIQYTDEENLQSSPSVIIYECSFKKDFTLPGDNYLLKEFGNKKFVIEVKNPARFDFAKQISIEKL", "aa_hexdigest": "4f4acc7bc32e6196752af0c056e6d40d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 22493.04690000001, "isoelectric_point": 5.501241493225096 }, "id": "NKOCPLAKPK_775", "locus": "GCA003260355_00739" }, { "type": "cds", "sequence": "contig_1", "start": 396075, "stop": 396452, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGAATAAACTCTTCAATCCCTCCCTTCCTACCCCCGCAGTAGAAAACGGCTATACCGTGGGAAACCCCATCGAAAAAGAAGGAAAACTGACTGCAGATGGAGATTTGCTGACCTATACACATACGTTAACGACGCCTGATGGAGAAAAATTCCTCCTCAAAAGTTCAACAATCTCGCTCAATAATTATACTATTATGACAGGGGATATTTTCAAGATTGAAGGAACAATCGAAGCCATCTACCAAGGACAAGCTCTCGTAGAGGTAAAAACGATCGAAAAGAATAATAGTAGTAAAAGCGAAACAGATTCTCCTTCCGAGAACAGCGAAGAAACCACAAATCCTGGCGTTTATCTAAACCAAGCAGGGATCTGA", "aa": "MKNKLFNPSLPTPAVENGYTVGNPIEKEGKLTADGDLLTYTHTLTTPDGEKFLLKSSTISLNNYTIMTGDIFKIEGTIEAIYQGQALVEVKTIEKNNSSKSETDSPSENSEETTNPGVYLNQAGI", "aa_hexdigest": "72d8fe122f2c4a84c6f771c77a3b9aae", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13573.92949999999, "isoelectric_point": 4.612450218200683 }, "id": "NKOCPLAKPK_776", "locus": "GCA003260355_00740" }, { "type": "cds", "sequence": "contig_1", "start": 397029, "stop": 397349, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02355.23" ], "nt": "ATGCCAGCTCCACTCATACTTCTCCAAGAGGAAAAAGTTAGCCCTTCGCTAGGAGACTCAGCATTTCAGGGAGCATTAATCGCTACTGGAGTAGGAACACTCGCTATCATTATTTACATGTTCTTCATCTACGGACGAAGAAAAGCCGCCGTAACAACAGCAACGATGTTCTTCTTTTTTATAGTGCTCTTAGCTACACTCAAGCTTATTGATTACGCATTATCACTCAGTGGAATCGCTGCTATTATCCTTTCTATTGGTATGGCAGTCGATGCGAATATCCTCATCTTTGAAAGAATGAACGAAGAAGTAAAGAGCTGA", "aa": "MPAPLILLQEEKVSPSLGDSAFQGALIATGVGTLAIIIYMFFIYGRRKAAVTTATMFFFFIVLLATLKLIDYALSLSGIAAIILSIGMAVDANILIFERMNEEVKS", "aa_hexdigest": "c2b3a0737b24b6797b2504ce76e54291", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02355.23", "name": "SecD_SecF_C", "length": 97, "aa_cov": 0.9150943396226415, "hmm_cov": 1.0, "evalue": 7.956942010878466e-14, "score": 47.92448043823242, "start": 9, "stop": 105 } ], "seq_stats": { "molecular_weight": 11462.605999999985, "isoelectric_point": 5.179930686950683 }, "id": "NKOCPLAKPK_777", "locus": "GCA003260355_00741" }, { "type": "cds", "sequence": "contig_1", "start": 397353, "stop": 397832, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF22599.2" ], "nt": "TTGAGAGAATCTTACCAAAATGGTTCAGGAGAAGAGGTGCCTATAGAAATTGACGGATTCCTCGTCTTCCGTGTAACAGCTAAAAACGAAATCTCAGCTATCGCAAACACAGGAAACAACTCCTCAGGTAATCAAACCCTCTACACTATTGATGAAATCTTCATTCAAGACAGACTCTCTCGAAAAATCGCACAGGGAAACGACGGAACCCCACTCAATGGAGCTTACTTTACCATCGCAAGACCTGAAACCACACAATTCGGACAGGCAGGAGTGAGTATTGGTTTTAATACCAAAGGAGGAGAGCTTTTCTGCCAAGTTACTCAAAACAACGTAGGAAAAAGTATGGCAATCTTCGTAGGAGGAGCACTTGCAACCCAAGCAAACATCCGTGAAAAAATCTGCGATGGGAAAGCCCAGATCTCTGGAGGATTTGGAAAAGATGAAGCAATCGCAATGGCAAAAGAACTCAATGCTTGA", "aa": "MRESYQNGSGEEVPIEIDGFLVFRVTAKNEISAIANTGNNSSGNQTLYTIDEIFIQDRLSRKIAQGNDGTPLNGAYFTIARPETTQFGQAGVSIGFNTKGGELFCQVTQNNVGKSMAIFVGGALATQANIREKICDGKAQISGGFGKDEAIAMAKELNA", "aa_hexdigest": "6be84703d52baa124b415bb149668ec2", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF22599.2", "name": "SecDF_P1_head", "length": 90, "aa_cov": 0.559748427672956, "hmm_cov": 1.0, "evalue": 2.5587687391740607e-20, "score": 68.92401123046875, "start": 70, "stop": 158 } ], "seq_stats": { "molecular_weight": 16918.710499999983, "isoelectric_point": 5.008447456359862 }, "id": "NKOCPLAKPK_778", "locus": "GCA003260355_00742" }, { "type": "cds", "sequence": "contig_1", "start": 398349, "stop": 398603, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAGCTAAAAGTCAAGTTAGTCTTGTATTTTTAGAGAAAAACCATATATTAACGCCGTTTTATTTTACATTTTATTGGCATATGAAAGGGTTTTTGACGACAAAAAGACTCACACTCGCAGTCCTATTTGTGATCAGTCTTTTTTTTGTATTTTTTGTAGGAAGGACCTTTAATTCTGAAACCTTTAAAAGGGATTGGATCCTTACCACAAAAGGAATTACTCATTTTAGAAAAGGACTCGATATCTCTTGA", "aa": "MQAKSQVSLVFLEKNHILTPFYFTFYWHMKGFLTTKRLTLAVLFVISLFFVFFVGRTFNSETFKRDWILTTKGITHFRKGLDIS", "aa_hexdigest": "912269a99b998838b17b1b2484cfc910", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10004.71779999999, "isoelectric_point": 10.284850883483884 }, "id": "NKOCPLAKPK_779", "locus": "GCA003260355_00743" }, { "type": "cds", "sequence": "contig_1", "start": 398991, "stop": 399266, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCGATTGAAAAGGCTTTTCTTGAGAATAAGGCACAAATTCTTGCACGAAAAAATAGTTCTCCTTGGGATGAGTTCGTTTTTAATGTTAGAGTATTTAATGAGGAAAGAGTATTGTCTCAGGCTCTTGATGAAATGATTGAGTTTGGAATTAGAAAATTTGTCTTTATCAATGATGGATCAAAGGATAACTCTCTTTCAATTTTAGAAGAAAAAAGGAGGGAACATCCAGATTTACTTTTTATTATACTTTCCCATACGATCAATAGAGGTTGA", "aa": "MAIEKAFLENKAQILARKNSSPWDEFVFNVRVFNEERVLSQALDEMIEFGIRKFVFINDGSKDNSLSILEEKRREHPDLLFIILSHTINRG", "aa_hexdigest": "1f19fd0623ea31e3bc6233d4ef6a1a20", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10666.084099999987, "isoelectric_point": 5.666415596008301 }, "id": "NKOCPLAKPK_780", "locus": "GCA003260355_00744" }, { "type": "cds", "sequence": "contig_1", "start": 399333, "stop": 399449, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTGGGTTTGATCCTGATGGACAGATGGATATCAAGGATATGTTGGCTTTTCAAAAAGCTATTCTCAAGCATCCTGAAGCTGATCTTTTTTTGGGTTCGAGATTTATTAAGTGA", "aa": "MVGFDPDGQMDIKDMLAFQKAILKHPEADLFLGSRFIK", "aa_hexdigest": "cd84bc06c34932a1fcff44ed8d24e37a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4324.051599999999, "isoelectric_point": 5.485894966125487 }, "id": "NKOCPLAKPK_781", "locus": "GCA003260355_00745" }, { "type": "cds", "sequence": "contig_1", "start": 399465, "stop": 399644, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCTAGAATGAGGAGGTGTATTTTGGCTATTTCTAGATTGGTTACAAGGATCTTTTATGGGGTTCGTGTTTCAGATCCGCATAATGGATATAGGGTTTTTACTATCCCTGCATTACAACAGATTACTTTAGAAGCGGATGGTATGCATTATGCTAACGAGCTGAATGAGCAAATTTGA", "aa": "MPRMRRCILAISRLVTRIFYGVRVSDPHNGYRVFTIPALQQITLEADGMHYANELNEQI", "aa_hexdigest": "64edf7bf2e58f8d7e425193409411879", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6875.9391, "isoelectric_point": 9.180571937561037 }, "id": "NKOCPLAKPK_782", "locus": "GCA003260355_00746" }, { "type": "cds", "sequence": "contig_1", "start": 399818, "stop": 400120, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTGAGAGTCTTCGCCATTCATTTATGTTTGCTCGGTCGAGTTTGACGAGCTTTGTTGCTGAGCAACCTTTTTTGTTTGTTATTATTGCTTTATTGGTCCTTTGGTCGATTATTTCTCATTTTATAAAATCTGCTCTGAAGAAAAAATATATTGACCCTTATGAACGTTCTAAAAATACCCAAAATAAGCAGTTTTATATTGAGTATTACAGAAGAAGTCAGTATGTTAATGTATTTGGGGTTGCTCTTGTTGTAATTCTGTTTTTTACGTATCTTTTGACTAAAGATAAGATCGTATGA", "aa": "MIESLRHSFMFARSSLTSFVAEQPFLFVIIALLVLWSIISHFIKSALKKKYIDPYERSKNTQNKQFYIEYYRRSQYVNVFGVALVVILFFTYLLTKDKIV", "aa_hexdigest": "aa4c3008e1c0df41c32f84d9343956f8", "start_type": "ATG", "rbs_motif": "TTCTT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11912.95689999999, "isoelectric_point": 9.820162773132324 }, "id": "NKOCPLAKPK_783", "locus": "GCA003260355_00747" }, { "type": "cds", "sequence": "contig_1", "start": 400286, "stop": 400609, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGGATCTCTGGGAAAAATGATTTTGGTGAAAATACGGGAGAGTTTTATATCATTCCTAATCATCAAATTTGGACCAATCCGGTTGTTAAGGTTGATCTTTCTCTAGATAATGTGACTAAACATTCCCTTACTTTAGTGTATGATGGTACAAATTTTTTGCTTCCTTTTGATGAATTTATTCAAGAATTAGAGGCTTTTCTTCTTGGATTGATGCCAATTAAAGCCGCATCTCAGGTTTCTTATTTTAAAAGTTATATAGGAGTCCGTTATAAGATTGATTTTACTTATGACAAAGATGGAAAACCTTGTGTTTGGATCTGA", "aa": "MGISGKNDFGENTGEFYIIPNHQIWTNPVVKVDLSLDNVTKHSLTLVYDGTNFLLPFDEFIQELEAFLLGLMPIKAASQVSYFKSYIGVRYKIDFTYDKDGKPCVWI", "aa_hexdigest": "07d8a2329d0848699f043dbd95348278", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12230.864399999988, "isoelectric_point": 4.939672279357908 }, "id": "NKOCPLAKPK_784", "locus": "GCA003260355_00748" }, { "type": "cds", "sequence": "contig_1", "start": 400841, "stop": 401038, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01170.24" ], "nt": "ATGATAAATATTGCACAAAACAATGCAAAACGTGCCAATGTAGGAGATATCATCTCTTTTCAGCAAAAAAACTTTTTAACGAGCAACTTTCACACAGAAGAAAACACTCGAATCATCACCAATCCTCCTTATGGGAAAAGAATCAAAAATCAGAATTTAGAGAACATATACACGAGATTAAACGATACTTTTGAGTGA", "aa": "MINIAQNNAKRANVGDIISFQQKNFLTSNFHTEENTRIITNPPYGKRIKNQNLENIYTRLNDTFE", "aa_hexdigest": "5fa5be631dc6a1115d8946cfc9ff4708", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01170.24", "name": "UPF0020", "length": 65, "aa_cov": 0.9846153846153847, "hmm_cov": 1.0, "evalue": 6.88723659610987e-14, "score": 48.43906021118164, "start": 1, "stop": 64 } ], "seq_stats": { "molecular_weight": 7613.412299999998, "isoelectric_point": 9.299773979187012 }, "id": "NKOCPLAKPK_785", "locus": "GCA003260355_00749" }, { "type": "cds", "sequence": "contig_1", "start": 401043, "stop": 401333, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTCGATATCCGAGGCAAAGATTGTATATTTGTTTTTGAAGATTCACGCTTTGGCATCTTGCTTGATTTTTTCAAAAGTTCAGGCTTCGAAATTTTTGAACTTCTCAAAAGCAAAGTTTCTCCGTGATCATGGTGCGATATTTTTGGCAAGGAGTGCTCCTTCTATAGCGATGGTTCCAGATCAGCAAAAGGGATCGATTAATGGGCTTTTGAATTTTCGGTTGGCGAGTAGAAGTAGAGCAACCGCGAGATTCTCTTTTAAGGGTGCTTCTCAGGCTTGGGTTTTGTAG", "aa": "MSISEAKIVYLFLKIHALASCLIFSKVQASKFLNFSKAKFLRDHGAIFLARSAPSIAMVPDQQKGSINGLLNFRLASRSRATARFSFKGASQAWVL", "aa_hexdigest": "d72f80c2528998db4ca0c31c86abdc93", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10592.377899999989, "isoelectric_point": 11.099795722961424 }, "id": "NKOCPLAKPK_786", "locus": "GCA003260355_00750" }, { "type": "cds", "sequence": "contig_1", "start": 402036, "stop": 402284, "strand": "+", "frame": 3, "gene": null, "product": "CYTH domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EBM3", "UniRef:UniRef90_A0A328EKG7" ], "nt": "ATGCATATAGAGTATGAGTTAAAAGTTTTGGATATTGATGTGGAGCAGGTGAGGGAAAAGTTATCAAATCTAGGAGCTTCTTATTTGGGAAAAAAGTTTTTTCGCAGATATACCTATGATATGCATCCACCTCAACCAGCGAAATGGATTAGGTTAAGGACGGATGGATCTGTTTCGACGTTAACCTGTAAACATATTCTTGATCCAGATGCAATAGATGGTGTAAAGGAATGGGAGTTTGAGGTCTGA", "aa": "MHIEYELKVLDIDVEQVREKLSNLGASYLGKKFFRRYTYDMHPPQPAKWIRLRTDGSVSTLTCKHILDPDAIDGVKEWEFEV", "aa_hexdigest": "0de8b6ae6fadd045c244be4362195bcc", "start_type": "ATG", "rbs_motif": "TTATT", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 402036, "stop": 402587, "observations": { "mutation": [ 402282 ], "stop": [ 402282 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EKG7", "query_cov": 1.0, "subject_cov": 0.44565217391304346, "identity": 1.0, "score": 173.0, "evalue": 1.25e-53, "gene_start": 1, "gene_end": 82, "reference_start": 1, "reference_end": 82, "reference_sequence": "MHIEYELKVLDIDVEQVREKLSNLGASYLGKKFFRRYTYDMHPPQPAKWIRLRTDGSVSTLTCKHILDPDAIDGVKEWEFEVGDFVVANQFLEQIGYKAKSYQENMRESYVFMNCTVEIDSRPLIPSYLEIEGNSEEEVLKVLQVLGLDRSDHTSQNTIQIYERYGICGMDQFPYLAFDCVIKK" }, "paralog": false, "identity": 0.994535519125683, "score": 373.0, "evalue": 5.56e-135, "description": "Internal stop codon at 402282. Nonsense mutation around 402282.", "psc": { "uniref90_id": "UniRef90_A0A328EKG7", "product": "CYTH domain-containing protein", "uniref50_id": "UniRef50_A0A328EBM3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EKG7", "UniRef:UniRef50_A0A328EBM3" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EBM3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EBM3" ], "product": "CYTH domain-containing protein" } }, "id": "NKOCPLAKPK_787", "locus": "GCA003260355_00751" }, { "type": "cds", "sequence": "contig_1", "start": 402351, "stop": 402590, "strand": "+", "frame": 3, "gene": null, "product": "CYTH domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EBM3", "UniRef:UniRef90_A0A328EKG7" ], "nt": "ATGCGTGAGAGTTATGTATTTATGAATTGTACAGTGGAAATAGATTCTCGACCTTTGATTCCAAGTTATCTTGAGATTGAGGGGAATTCTGAAGAAGAAGTGCTCAAGGTTTTGCAGGTTTTGGGGCTTGATCGTTCTGATCATACCTCCCAAAATACTATTCAGATTTATGAGAGATATGGAATTTGTGGTATGGATCAATTTCCTTATCTTGCTTTTGATTGTGTGATAAAAAAGTAG", "aa": "MRESYVFMNCTVEIDSRPLIPSYLEIEGNSEEEVLKVLQVLGLDRSDHTSQNTIQIYERYGICGMDQFPYLAFDCVIKK", "aa_hexdigest": "701ebae2974d4d4fe7a130b166950693", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 402051, "stop": 402590, "observations": { "mutation": [ 402282 ], "stop": [ 402282 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EKG7", "query_cov": 1.0, "subject_cov": 0.42934782608695654, "identity": 1.0, "score": 163.0, "evalue": 6.98e-50, "gene_start": 1, "gene_end": 79, "reference_start": 106, "reference_end": 184, "reference_sequence": "MHIEYELKVLDIDVEQVREKLSNLGASYLGKKFFRRYTYDMHPPQPAKWIRLRTDGSVSTLTCKHILDPDAIDGVKEWEFEVGDFVVANQFLEQIGYKAKSYQENMRESYVFMNCTVEIDSRPLIPSYLEIEGNSEEEVLKVLQVLGLDRSDHTSQNTIQIYERYGICGMDQFPYLAFDCVIKK" }, "paralog": false, "identity": 0.994413407821229, "score": 364.0, "evalue": 2.24e-131, "description": "Internal stop codon at 402282. Nonsense mutation around 402282.", "psc": { "uniref90_id": "UniRef90_A0A328EKG7", "product": "CYTH domain-containing protein", "uniref50_id": "UniRef50_A0A328EBM3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EKG7", "UniRef:UniRef50_A0A328EBM3" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EBM3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EBM3" ], "product": "CYTH domain-containing protein" } }, "id": "NKOCPLAKPK_788", "locus": "GCA003260355_00752" }, { "type": "cds", "sequence": "contig_1", "start": 402815, "stop": 403870, "strand": "+", "frame": 2, "gene": "dnaK", "product": "molecular chaperone DnaK", "db_xrefs": [ "GO:0005524", "GO:0006457", "GO:0016887", "SO:0001217", "UniRef:UniRef50_P17820", "UniRef:UniRef90_A0A839JVH0" ], "nt": "ATGGCAAAAATTATTGGAATTGATCTTGGAACGACCAACTCTTGTGTATCCTATATGCTCGGTGATAAGTCCGAAGTGATCGCAAATGCAGAAGGAAATAGGACTACGCCTTCTATCGTGTATATCAAGGGGGATGAATTGCTTGTTGGAGATTTGGCAAAGAGAAAGGCGATTTTGGAGCCAAAAAATGTTGTATATGAGGTGAAAAGATGGATCGGTAGAAAATATTCTGAGGTAAAAAATGAACTTGCTAATATGGCTTATGAAACTAAAGAAGGAAGTGATGGAGGAATCTTAATCGTTGTTGATGGAAAAGAATATAAACCTGAACAGATTTCTGCATTCATTCTCCAAAAACTCAGATCTGATGCGGAGAAATTCCTTGGAGAAACGATTACTCAGGCGGTGATTACTGTTCCTGCTTACTTCAACGACTCTCAGAGAAATGCAACCAAGGCTGCTGGAGAAATTGCAGGGCTCAAGGTAGAAAGAATCATCAACGAACCGACTGCTGCGGCTCTTGCTTATGGAGAAGGGAAGAAAGCTGATGAAAAGATCGTCGTATTCGATCTTGGAGGAGGAACTTTTGATGTAACGGTGCTTGACATCGGATCAGAAGGGACTTTCCAGGTACTCTCTACTTCTGGAGATACGCAGCTTGGAGGTGCAGATTGGGATCAAAGAATTATTGATTATGTTGTTGATGCTTTCAAAACCAAGGAAGGAATCGATCTCAAAGGAAATGCAATGGCAATGCAGAGAATTAAAGATGAGGCTGAAAAAGCAAAAAAGCAGCTTTCCTCTGTAGAAAGAGTTGATATCTCTATTCCGTTTATTACGACAGGAACCGACGGACTTCCTAGGAATCTTGATGAATCGTTGACTAGGGCTCAATTTGAGAATATTACTTCAGATTTGTTTACAAGATGTAAGGAGCCTGTTTTGAATGCTTTGAGAGATTCAAAATTAGAAAAATCTGAAATCAACGACATCATTTTGGTGGGAGGTTCTACGAGAATGCTCAAAGTAAAGGCAATCGTAAAGGAAATCTTCTGA", "aa": "MAKIIGIDLGTTNSCVSYMLGDKSEVIANAEGNRTTPSIVYIKGDELLVGDLAKRKAILEPKNVVYEVKRWIGRKYSEVKNELANMAYETKEGSDGGILIVVDGKEYKPEQISAFILQKLRSDAEKFLGETITQAVITVPAYFNDSQRNATKAAGEIAGLKVERIINEPTAAALAYGEGKKADEKIVVFDLGGGTFDVTVLDIGSEGTFQVLSTSGDTQLGGADWDQRIIDYVVDAFKTKEGIDLKGNAMAMQRIKDEAEKAKKQLSSVERVDISIPFITTGTDGLPRNLDESLTRAQFENITSDLFTRCKEPVLNALRDSKLEKSEINDIILVGGSTRMLKVKAIVKEIF", "aa_hexdigest": "6ba6fea0270700cbdcb290718d27af22", "start_type": "ATG", "rbs_motif": "TTTA", "psc": { "uniref90_id": "UniRef90_UPI002942B975", "query_cov": 1.0, "subject_cov": 0.9887323943661972, "identity": 0.5529999999999999, "score": 386.0, "evalue": 8.94e-131, "valid": false, "product": "Hsp70 family protein", "uniref50_id": "UniRef50_A0A0C1ERJ6" }, "genes": [ "dnaK" ], "truncated": "3-prime", "pseudogene": { "start": 402815, "stop": 404173, "observations": { "mutation": [ 403868 ], "stop": [ 403868 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A839JVH0", "query_cov": 1.0, "subject_cov": 0.560702875399361, "identity": 0.929, "score": 637.0, "evalue": 1.43e-225, "gene_start": 1, "gene_end": 351, "reference_start": 1, "reference_end": 351, "reference_sequence": "MAKIIGIDLGTTNSCVSYMLGDKSEVIANAEGNRTTPSIVYIKGDELLVGDLAKRKAILEPKNVIYEVKRWIGRKYSEVKDELKNMTYDTKEGSDGGVLIVVDGKEYKPEQISAFILQKLKTDAEKFLGETITQAVITVPAYFNDSQRNATKAAGEIAGLKVERIINEPTAAALAYGEGKKSDEKIVVFDLGGGTFDVTVLEIGAEGTFQVLSTSGDTQLGGADWDQRIINYIVDAFKTKEGIDLRDNSMAMQRVKDEAEKAKKQLSSVERVDISIPFITTGADGLPRKLDETLTRAQFENITSDLFSRCKEPVLNALNDSKLNKSEINDIILVGGSTRMLKVKEIVKDIFGKEPKSTVNPDESVAQGAAIQGGIVQGDVKDILLLDVTPLSLAVEVEGGIAHVMIPRNTTIPAKKSNIYTTATDNQNAVTVHVTQGERQFARDNKSLGMFNLEGIPPMRRGQPQIEVTFDIDANGILKVHAQEKSTGKSQEVTIQGSTNISDEDIAKAKADAEKFAEEDRKRKELVESKNKLESLIYQIENLMNEQKDKISDEDKEKIQKLIADGKAVKDKEDAEKSHIDAEIERIQNQFQELYQKYQVQPTPDQPQNGADNASGPKDGEVIDAD" }, "paralog": false, "identity": 0.9402654867256637, "score": 835.0, "evalue": 6.56e-306, "description": "Internal stop codon at 403868. Nonsense mutation around 403868.", "psc": { "uniref90_id": "UniRef90_A0A839JVH0", "gene": "dnaK", "product": "molecular chaperone DnaK", "uniref50_id": "UniRef50_P17820", "go_ids": [ "GO:0005524", "GO:0006457", "GO:0016887" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A839JVH0", "UniRef:UniRef50_P17820", "GO:0005524", "GO:0006457", "GO:0016887" ] }, "pscc": { "uniref50_id": "UniRef50_P17820", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P17820" ], "product": "Chaperone protein DnaK" } }, "id": "NKOCPLAKPK_789", "locus": "GCA003260355_00753" }, { "type": "cds", "sequence": "contig_1", "start": 403940, "stop": 404695, "strand": "+", "frame": 2, "gene": "dnaK", "product": "molecular chaperone DnaK", "db_xrefs": [ "GO:0005524", "GO:0006457", "GO:0016887", "SO:0001217", "UniRef:UniRef50_P17820", "UniRef:UniRef90_A0A839JVH0" ], "nt": "GTGCAAGGAGATGTGAAAGATATTCTTTTGCTTGATGTGACTCCGCTTTCTCTTGCGGTTGAAGTTGAGGGAGGAATCGCTCATGTGATGATCCCGAGAAATACTACTATTCCAGCGAAAAAGTCTAATATCTATACCACAGCTGCGGACAATCAAAACGCCGTAACCGTGCACGTGACGCAAGGAGAAAGACAGTTTGCAAGAGATAATAAATCGCTTGGAATGTTTAATCTTGAAGGCATTCCTCCAATGAGAAGAGGACAGCCACAAATTGAGGTAACCTTTGATATTGATGCGAACGGAATCCTTAAGGTGCATGCTCAGGAAAAATCTACTGGAAAATCTCAGGAAGTGACGATTCAGGGTTCTACCAATATTTCTGATGAAGATATTGCAAAAGCTAAGGCTGATGCAGAAAAATTTGCAGAGGAAGATAGAAAAAGGAAGGAAATTGTGGAATCTAAAAATAAGCTAGAATCCCTTGTTTACCAGATGGAAAACCTCCTTACTGATCAAAAAGATAAGATTTCTGAGGAAGATAAAGAAAAAATCCAAAAGCTGATTACTGATGGAAAGGCAGTGAAAGATAAGGAGGATGCTGAGAAAACTCATATTGATTCTGAAATTGAGAGGATTCAAAGTCAGTTTCAGGAGCTTTATCAAAAATATCAGGTACAACCTGCTCCAGATCAACCTCAAGCTGAAGCTTCTGAAGGAGAAAAACCAGCTGATGGAGAAGTGATTGATGCTGACTAG", "aa": "MQGDVKDILLLDVTPLSLAVEVEGGIAHVMIPRNTTIPAKKSNIYTTAADNQNAVTVHVTQGERQFARDNKSLGMFNLEGIPPMRRGQPQIEVTFDIDANGILKVHAQEKSTGKSQEVTIQGSTNISDEDIAKAKADAEKFAEEDRKRKEIVESKNKLESLVYQMENLLTDQKDKISEEDKEKIQKLITDGKAVKDKEDAEKTHIDSEIERIQSQFQELYQKYQVQPAPDQPQAEASEGEKPADGEVIDAD", "aa_hexdigest": "906b2c2fcd7b4b2632b74d304adf059d", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0035AE99B7", "query_cov": 1.0079681274900398, "subject_cov": 0.9405204460966543, "identity": 0.518, "score": 219.0, "evalue": 5.31e-68, "valid": false, "product": "Hsp70 family protein", "uniref50_id": "UniRef50_A0A7S4AHY6" }, "genes": [ "dnaK" ], "truncated": "5-prime", "pseudogene": { "start": 403640, "stop": 404695, "observations": { "mutation": [ 403868 ], "stop": [ 403868 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A839JVH0", "query_cov": 1.0, "subject_cov": 0.4009584664536741, "identity": 0.912, "score": 432.0, "evalue": 1.15e-146, "gene_start": 1, "gene_end": 251, "reference_start": 376, "reference_end": 626, "reference_sequence": "MAKIIGIDLGTTNSCVSYMLGDKSEVIANAEGNRTTPSIVYIKGDELLVGDLAKRKAILEPKNVIYEVKRWIGRKYSEVKDELKNMTYDTKEGSDGGVLIVVDGKEYKPEQISAFILQKLKTDAEKFLGETITQAVITVPAYFNDSQRNATKAAGEIAGLKVERIINEPTAAALAYGEGKKSDEKIVVFDLGGGTFDVTVLEIGAEGTFQVLSTSGDTQLGGADWDQRIINYIVDAFKTKEGIDLRDNSMAMQRVKDEAEKAKKQLSSVERVDISIPFITTGADGLPRKLDETLTRAQFENITSDLFSRCKEPVLNALNDSKLNKSEINDIILVGGSTRMLKVKEIVKDIFGKEPKSTVNPDESVAQGAAIQGGIVQGDVKDILLLDVTPLSLAVEVEGGIAHVMIPRNTTIPAKKSNIYTTATDNQNAVTVHVTQGERQFARDNKSLGMFNLEGIPPMRRGQPQIEVTFDIDANGILKVHAQEKSTGKSQEVTIQGSTNISDEDIAKAKADAEKFAEEDRKRKELVESKNKLESLIYQIENLMNEQKDKISDEDKEKIQKLIADGKAVKDKEDAEKSHIDAEIERIQNQFQELYQKYQVQPTPDQPQNGADNASGPKDGEVIDAD" }, "paralog": false, "identity": 0.9145299145299145, "score": 630.0, "evalue": 1.3e-226, "description": "Internal stop codon at 403868. Nonsense mutation around 403868.", "psc": { "uniref90_id": "UniRef90_A0A839JVH0", "gene": "dnaK", "product": "molecular chaperone DnaK", "uniref50_id": "UniRef50_P17820", "go_ids": [ "GO:0005524", "GO:0006457", "GO:0016887" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A839JVH0", "UniRef:UniRef50_P17820", "GO:0005524", "GO:0006457", "GO:0016887" ] }, "pscc": { "uniref50_id": "UniRef50_P17820", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P17820" ], "product": "Chaperone protein DnaK" } }, "id": "NKOCPLAKPK_790", "locus": "GCA003260355_00754" }, { "type": "cds", "sequence": "contig_1", "start": 405058, "stop": 405228, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTACCTCGATATTAGAAGCAAACCAAGGAACTCCTCCTCAAAACACAGAAATTGCATTCTCGCTCAATCCTCAAAACATCATCTCAAAATACGAAAAAGGAACAGCACCACTAAAAGCCAGGATAAAAGCCATAAATACGCTCCTCGAGAAAGGTTATAAGGTATGA", "aa": "MITSILEANQGTPPQNTEIAFSLNPQNIISKYEKGTAPLKARIKAINTLLEKGYKV", "aa_hexdigest": "c425bfbf0dbe93e2f0474e09ef74d1c2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6158.085400000002, "isoelectric_point": 9.522770118713382 }, "id": "NKOCPLAKPK_791", "locus": "GCA003260355_00755" }, { "type": "cds", "sequence": "contig_1", "start": 405361, "stop": 405741, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTATTTATCTCGAATCCAAAGCAAAAGACTACCCTCTTAGCAAAAAAATCCTCAAAAAGTTATCTCATGCCAACATTCTAGAAATAGATCATTACAAGAATATTTTTGATAAAAACATAGGAAATCAAAAAGTAGAGCCAGCTATTATCTTAGCCAAACAAGAACACCCCAAGCTAATTCCTGTTCCAAATCATTATGGTTATCCAGGGAAATCGTTTTTCTTTAAGACAAGCCTAAATTGTGTATTTGACTGTGATTATTGCTATCTTAAAGGGAATTTTAAAAGCCCATATCCTACAATTTTCGTAAATTATGACGACATAAAAAATCAGATAAAAAGCTCAATCTCAGAGCTCAGAAATCAAGGATATAACTGA", "aa": "MLIYLESKAKDYPLSKKILKKLSHANILEIDHYKNIFDKNIGNQKVEPAIILAKQEHPKLIPVPNHYGYPGKSFFFKTSLNCVFDCDYCYLKGNFKSPYPTIFVNYDDIKNQIKSSISELRNQGYN", "aa_hexdigest": "87427cf12539c7c7227a3d06bfdef850", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14667.787399999981, "isoelectric_point": 9.139312171936037 }, "id": "NKOCPLAKPK_792", "locus": "GCA003260355_00756" }, { "type": "cds", "sequence": "contig_1", "start": 405940, "stop": 406215, "strand": "-", "frame": 1, "gene": null, "product": "Thioredoxin", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q184M2" ], "nt": "ATGGCAAAAATCATCGCTTCAAAAGCAGAATTTGAGCAAATTCTTAATGAGTTTCAGGGAGTAATCGTAGTTGACTTTTTTGCAACACGATGTGGTCCATGCAAGATGCTCTCACCAGTTATGGAAGAACTTTCTACGCAATATGATGAAAATCAGGTAAAAATTCTGAAAGTAGATGTAGATCAGGTTCCAGAATTGGCAGAAGAATACGGTATCGTTTCTATACCAACAGTCTTTTTCGGAACAAATCACAAGCTTGAAGAGGGCGTAATGTGA", "aa": "MAKIIASKAEFEQILNEFQGVIVVDFFATRCGPCKMLSPVMEELSTQYDENQVKILKVDVDQVPELAEEYGIVSIPTVFFGTNHKLEEGVM", "aa_hexdigest": "1f7c4ad29d63e1146eca2263aed1dd11", "start_type": "ATG", "rbs_motif": "TTATT", "psc": { "uniref90_id": "UniRef90_A0A1M6D679", "query_cov": 1.0, "subject_cov": 0.8584905660377359, "identity": 0.505, "score": 87.4, "evalue": 1.43e-20, "valid": false, "gene": "trxA", "product": "thioredoxin", "uniref50_id": "UniRef50_Q184M2", "go_ids": [ "GO:0015035" ] }, "pscc": { "uniref50_id": "UniRef50_Q184M2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q184M2" ], "product": "Thioredoxin" }, "genes": [], "id": "NKOCPLAKPK_793", "locus": "GCA003260355_00757" }, { "type": "cds", "sequence": "contig_1", "start": 406864, "stop": 407211, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EID7", "UniRef:UniRef90_A0A328EID7" ], "nt": "TTGAAGGCTACGATCTGGTTGCATAGTTCTACAGGGAAAATTCATGTTTTTTCACTCGTCTTTGATCAAGTATTCGTTCACGTATTTGTTCACGTATTTTGGGATCACGTGAGTAATGCTGTGTTTCAGGAGTTAAGCTCTTTTGCCATAGCATTCCCAGAGAGAGAGGATGGTAGTTCTCAGGAAAGGGCAACTTCTGTGGATCAGGATCCTATGTTTTCAGCACTAGTGCTGCCTGTAGATATTCAGTCTTTAGGTTTTTTTGTGCCACATGCGGAAAGTAGTATAACAAGTCCTATTATTGAGATGAAGAGGTGTGTTTTTCTCATTTTTTGCTTTGCCTATTAA", "aa": "MKATIWLHSSTGKIHVFSLVFDQVFVHVFVHVFWDHVSNAVFQELSSFAIAFPEREDGSSQERATSVDQDPMFSALVLPVDIQSLGFFVPHAESSITSPIIEMKRCVFLIFCFAY", "aa_hexdigest": "f5797985a7bbf053c63319e11bbe1131", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A328EID7", "query_cov": 1.0, "subject_cov": 0.9745762711864406, "identity": 0.991, "score": 204.0, "evalue": 3.85e-66, "valid": true, "uniref50_id": "UniRef50_A0A328EID7" }, "pscc": { "uniref50_id": "UniRef50_A0A328EID7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EID7" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13043.830999999987, "isoelectric_point": 5.305317497253417 }, "id": "NKOCPLAKPK_794", "locus": "GCA003260355_00758" }, { "type": "cds", "sequence": "contig_1", "start": 407440, "stop": 407847, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01406.26" ], "nt": "GTGAAGTATCGGTTGCACAACGAATTTTTGCAGGTAGATGGAGGGAAAATGAGTAAATCACTCGGAAATATCTACTCTTTGGAAGATATTAAGGCCAAAGGCTATTCTCCGCTTGATTTGAGATATTTCTATTTTAAGGCTCAGTATGGAAACTTTCAAAACTTCACTTGGGAAGCTTTAGAGCAAGCCAAAAATGAAAGAGCAGGTTTGATCAAGAAAATCCAAAATCTGACTCAAGCTTCTCAGCAAGCAAGCACTGAAGATTGGAAACTTCGCTTTGATGAAGCCCTTGCTGATAATTTCAATACCCCAAGACTCCTTTCTGAGCTGCATTCTGCACTTTCCAAGTCAGGAAGTGAGCTTTTACCGCTTCTACACGAATTAGAAGAAAAAGTTCTAAAAATCTGA", "aa": "MKYRLHNEFLQVDGGKMSKSLGNIYSLEDIKAKGYSPLDLRYFYFKAQYGNFQNFTWEALEQAKNERAGLIKKIQNLTQASQQASTEDWKLRFDEALADNFNTPRLLSELHSALSKSGSELLPLLHELEEKVLKI", "aa_hexdigest": "5e7ec1d65aa969faf20b2a7c2584bc3e", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01406.26", "name": "tRNA-synt_1e", "length": 64, "aa_cov": 0.4740740740740741, "hmm_cov": 0.984375, "evalue": 5.775177618290066e-13, "score": 45.28772735595703, "start": 2, "stop": 65 } ], "seq_stats": { "molecular_weight": 15602.525199999995, "isoelectric_point": 6.840481758117675 }, "id": "NKOCPLAKPK_795", "locus": "GCA003260355_00759" }, { "type": "cds", "sequence": "contig_1", "start": 407866, "stop": 408678, "strand": "-", "frame": 1, "gene": "cysS", "product": "cysteine--tRNA ligase", "db_xrefs": [ "EC:6.1.1.16", "GO:0004817", "GO:0005524", "GO:0005737", "GO:0006423", "KEGG:K01883", "SO:0001217", "UniRef:UniRef50_A0A288GSX8", "UniRef:UniRef90_A0A328EGR0" ], "nt": "ATGCAGATTAAACTCTACAACACCCTCACTCGTGAGAAAGAAAACTTTATCCCACTGATGCAAGATCCTAACTATCATGGGGAGAAAAAGGATTTTGTCGGAATGTATTCCTGTGGACCTACCGTCTATTATACTCCGCATATTGGAAACCTCAGAGCGACTTTTACTGCGGATTTGATCAGGAATGTGCTGAAGTATTTTGGCTATCCAGTGAAAGCGGTGATGAATATTACCGATGTAGGGCATCTCGTTTCTGATGGTGATGAGGGCGAGGATAAGCTTGAAAAAGGAGCGAAAAGAGACGGAATCTCCGCTTGGGAAGTCGCAAAAAAATATGAAGAAATCTTCCTGACTGCGATGAATGAGCTGAATATTCAGACTTTTGATGTGATGCCAAGAGCGACTGAGCATATCGCCGAGCAAATCGCACTTGTGCAGAGCCTAGAAGCGAAAGGCTACACCTACGAAGTTCCGGGTGATGGAATCTATATGGATACGAGTAAAGTAGAGGATTATGGGAAACTCATGGGACCAAACTATAAAAAAAGACTTGAGGACCTGAGGGCAGGAGAGCGTGTTGATATGAAAAGTAAGAAAAATCCGACAGACTTCGCTTTATGGAAATTCTCTCTGACCGATGAGAAAAGGGAAATGGAGCGAGATTCACCATGGGGAGTTGGTTTTCCGGGTTGGCATATAGAATGTAGTGCGATGTCTAGTAAATATCTTGGTGAACAGTTTGATATTCATCATGGGGGAGCTGATCACATCACGATTCATCATCCAAACGAGATTGCTCAATCAGAATGCTGA", "aa": "MQIKLYNTLTREKENFIPLMQDPNYHGEKKDFVGMYSCGPTVYYTPHIGNLRATFTADLIRNVLKYFGYPVKAVMNITDVGHLVSDGDEGEDKLEKGAKRDGISAWEVAKKYEEIFLTAMNELNIQTFDVMPRATEHIAEQIALVQSLEAKGYTYEVPGDGIYMDTSKVEDYGKLMGPNYKKRLEDLRAGERVDMKSKKNPTDFALWKFSLTDEKREMERDSPWGVGFPGWHIECSAMSSKYLGEQFDIHHGGADHITIHHPNEIAQSEC", "aa_hexdigest": "4c588deb98477f96c6d5f47fcb1e484b", "start_type": "ATG", "rbs_motif": "TTATT", "psc": { "uniref90_id": "UniRef90_A0A0G0VWE4", "query_cov": 0.9629629629629629, "subject_cov": 1.0526315789473684, "identity": 0.5579999999999999, "score": 291.0, "evalue": 2.41e-96, "valid": false, "product": "Cysteine-tRNA ligase", "uniref50_id": "UniRef50_A0A948NNP8" }, "genes": [ "cysS" ], "truncated": "5-prime", "pseudogene": { "start": 407563, "stop": 408678, "observations": { "mutation": [ 407862, 407868 ], "stop": [ 407862, 407868 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EGR0", "query_cov": 1.0, "subject_cov": 0.569620253164557, "identity": 0.981, "score": 553.0, "evalue": 2.63e-196, "gene_start": 1, "gene_end": 270, "reference_start": 1, "reference_end": 270, "reference_sequence": "MQIKLYNTLTREKEDFTPLMQDPNYHGEKKDFVGMYSCGPTVYYTPHIGNLRATFTADLIRNVLKYFGYPVKAVMNITDVGHLVSDGDEGEDKLEKGAKRDGISAWEVAKKYEEIFLTAMNELNIQTFDVMPRATEHIAEQIALVQHLEAKGYTYEVPGDGIYMDTSKVEDYGKLMGLNYKKRLEDLRAGERVDMKGKKNPTDFALWKFSLTDEKREMERDSPWGVGFPGWHIECSAMSSKYLGEQFDIHHGGADHITIHHPNEIAQSECGFGKKPWVKYRLHNEFLQVDGGKMSKSLGNIYSLEDIKAKGYSPLDLRYFYFKAQYGNFQNFTWEALDQAKNERVGLIKKIQNLTQTSEQASTEDWKLRFDDALADNFNTPKLLSELHSAFSKSGTELLPLLHELEEKVLKIGLFSRETPTSQEVPETIISLAEQRKQAKAEKNYALADELRTQITQAGWEIKDTKEGYELSKS" }, "paralog": false, "identity": 0.9703504043126685, "score": 748.0, "evalue": 3.52e-275, "description": "Internal stop codon at 407862, 407868. Nonsense mutation around 407862, 407868.", "psc": { "uniref90_id": "UniRef90_A0A328EGR0", "gene": "cysS", "product": "cysteine--tRNA ligase", "ec_ids": [ "6.1.1.16" ], "uniref50_id": "UniRef50_A0A288GSX8", "kegg_orthology_id": "K01883", "go_ids": [ "GO:0004817", "GO:0005524", "GO:0005737", "GO:0006423" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EGR0", "EC:6.1.1.16", "UniRef:UniRef50_A0A288GSX8", "KEGG:K01883", "GO:0004817", "GO:0005524", "GO:0005737", "GO:0006423" ] }, "pscc": { "uniref50_id": "UniRef50_A0A288GSX8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A288GSX8" ], "product": "Cysteine--tRNA ligase" } }, "id": "NKOCPLAKPK_796", "locus": "GCA003260355_00760" }, { "type": "cds", "sequence": "contig_1", "start": 408780, "stop": 409037, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EJR4", "UniRef:UniRef90_A0A328EJR4" ], "nt": "ATGAAAAAGACAAAGTTTCCCTTCAATCAGATTTGTCGTTTGCAACTTGAGGTAACTGAGAAGGAACAGCTTCTTGAGAAATTGCTCGCCATTGCTTCTCGTGATGGTGTGGAAAAGCTTCGAGTTGAACTTGAGGAGCTTTCCTGGCAGATTGATCGTATGGAGGAGCAGGGAATAGCTCTTTCAAACTATCCTCTTTCGATGCAAGTTAGTGATCTGATTTCTAGATGTGATCGTTTTATGAAGCAGGGGAGTTGA", "aa": "MKKTKFPFNQICRLQLEVTEKEQLLEKLLAIASRDGVEKLRVELEELSWQIDRMEEQGIALSNYPLSMQVSDLISRCDRFMKQGS", "aa_hexdigest": "54d62bdbd687014ec8c29f44d581a2ad", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A328EJR4", "query_cov": 1.0, "subject_cov": 0.8673469387755102, "identity": 1.0, "score": 164.0, "evalue": 2.14e-51, "valid": true, "uniref50_id": "UniRef50_A0A328EJR4" }, "pscc": { "uniref50_id": "UniRef50_A0A328EJR4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EJR4" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9961.479199999994, "isoelectric_point": 5.47026424407959 }, "id": "NKOCPLAKPK_797", "locus": "GCA003260355_00761" }, { "type": "cds", "sequence": "contig_1", "start": 409624, "stop": 409815, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A929ZUU4" ], "nt": "ATGAGAGAAGATACCCTCAATAAAATCACGAAAGAAACCAATCTCAAACCAGCATTTAAAGAAAATATGCCACAAACAACAATTATCATTGATCTAAAGAAAACGGATGAAGAGCTACTAAATGAAATGCAAAATGGCTCAGCAGAAAAAATCAAAAAAGCCATTAAAAAAGGTATAAAAATCAGAGAATGA", "aa": "MREDTLNKITKETNLKPAFKENMPQTTIIIDLKKTDEELLNEMQNGSAEKIKKAIKKGIKIRE", "aa_hexdigest": "31531f5eb8fedc68b0ee9d97c83ad31a", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A929ZUU4", "query_cov": 0.9841269841269841, "subject_cov": 0.9841269841269841, "identity": 0.613, "score": 70.5, "evalue": 7.49e-15, "valid": false, "uniref50_id": "UniRef50_A0A929ZUU4" }, "pscc": { "uniref50_id": "UniRef50_A0A929ZUU4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A929ZUU4" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7300.4779, "isoelectric_point": 9.301321220397949 }, "id": "NKOCPLAKPK_798", "locus": "GCA003260355_00762" }, { "type": "cds", "sequence": "contig_1", "start": 410303, "stop": 410812, "strand": "+", "frame": 2, "gene": null, "product": "Isochorismatase-like domain-containing protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DC008C9", "UniRef:UniRef100_A0A328EGJ4", "UniRef:UniRef50_A0A328EGJ4", "UniRef:UniRef90_A0A328EGJ4" ], "nt": "ATGCAAGAGACAGCATTAGTGATTATCAATATGCAAATGGCATGGACAGATAAAGATTCTGAATACTATCTTGGGAATCTTGATGCAATGGTAGAAAAAATGAACTACTTAATTGCCTATGCTCGTGAACTTGGCTATAAAATTATTTTTGTCAAGCATCACGAAACGGAAGGAGTTTTTTCTGAAAAAAGTCCTTTTTCTGATTTTATCCCGGAATTAGCTGTTGAGAAGGATGATATTATTATTCCTAAATATAAGGTAAGTAGCTTTTATCAGACTAAACTTGAGGATGAGTTAACTGGGGTGAAAAATCTGGTTGTTTGTGGGGCTTTGACGAATTTGTGTGTTAGGATGTTTGTAGAGGAGGCTTACGATAGAGAGTATAGGATTGCTTTGATCGACGATTTGACGGTAACTTATTCGGAGGAATTGCATGATACGACATTGCAAGATTTGGTAGATACGAGGTCTGGTTTGACGATTTTGCCTTTGGAAAAGTTTATAGATTAG", "aa": "MQETALVIINMQMAWTDKDSEYYLGNLDAMVEKMNYLIAYARELGYKIIFVKHHETEGVFSEKSPFSDFIPELAVEKDDIIIPKYKVSSFYQTKLEDELTGVKNLVVCGALTNLCVRMFVEEAYDREYRIALIDDLTVTYSEELHDTTLQDLVDTRSGLTILPLEKFID", "aa_hexdigest": "07a4ae594d9e4abd6a122c3c4b24366b", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000DC008C9", "uniref100_id": "UniRef100_A0A328EGJ4", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DC008C9", "UniRef:UniRef100_A0A328EGJ4" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EGJ4", "uniref90_id": "UniRef90_A0A328EGJ4" }, "psc": { "uniref90_id": "UniRef90_A0A328EGJ4", "product": "Isochorismatase-like domain-containing protein", "uniref50_id": "UniRef50_A0A328EGJ4" }, "pscc": { "uniref50_id": "UniRef50_A0A328EGJ4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EGJ4" ], "product": "Isochorismatase-like domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_799", "locus": "GCA003260355_00763" }, { "type": "cds", "sequence": "contig_1", "start": 410834, "stop": 411319, "strand": "+", "frame": 2, "gene": null, "product": "Methyltransferase domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4KR61", "UniRef:UniRef90_A0A328EKH8" ], "nt": "ATGATTATGGAAGGAGCGAAGTTAGTGAGTAGATTGATTGAAAAATATCAACCGAAAACTATTTTAGATTTGGGAGCTGGGAAAGGGTATTTTAGTATTTTGGCCGCGAGTTATGGAGCTCAGGTAGATGCTGTAGAAGATACGAGTATTAGGAATCTTTATCCAGATTATCTTAAGGTTCATCCTAGTGTTTCTTTCTATGAAAGTAAACTTGATGAGTTTGAAATTACCAAAAATTACGATCTTGTTATTGCAAAACATGTTGTGATGTATATGGATAAAAAGTATGTTTTGGAGAGGCTGATTCCTTCGGTTTATGAACATCTCAATAAAGGAGGACTTTTCTTCTTGACCTACCATCATTCGGATTCTGAACTCTTAAAAGAAGAGGCGGGGATTGTACATTATACGCTTGATGATTTTAAATCATTTAAGGGAAGCTTCTCGGTAAAGGATTTTGGGGATTATTCTAATCCTGCTCCTTGA", "aa": "MIMEGAKLVSRLIEKYQPKTILDLGAGKGYFSILAASYGAQVDAVEDTSIRNLYPDYLKVHPSVSFYESKLDEFEITKNYDLVIAKHVVMYMDKKYVLERLIPSVYEHLNKGGLFFLTYHHSDSELLKEEAGIVHYTLDDFKSFKGSFSVKDFGDYSNPAP", "aa_hexdigest": "08d9c4c59e19a5fd83726d4f33493f57", "start_type": "ATG", "rbs_motif": "TTATT", "psc": { "uniref90_id": "UniRef90_A0A328EKH8", "query_cov": 1.0, "subject_cov": 0.9096045197740112, "identity": 0.981, "score": 317.0, "evalue": 2.74e-109, "valid": true, "product": "Methyltransferase domain-containing protein", "uniref50_id": "UniRef50_A0A2W4KR61" }, "pscc": { "uniref50_id": "UniRef50_A0A2W4KR61", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4KR61" ], "product": "Methyltransferase domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_800", "locus": "GCA003260355_00764" }, { "type": "cds", "sequence": "contig_1", "start": 411396, "stop": 411644, "strand": "+", "frame": 3, "gene": null, "product": "DUF1653 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_K9DMN5", "UniRef:UniRef90_A0A660MWX8" ], "nt": "GTGATGAGAGAACTGAAAATTCATGGTATTTACAGACATTTTAAGGGAGATCTTTATTTGGTGGAAGATGTCGCTACGCATTCTGAAACGAAAGAAAAATATGTCGTGTATAGGGCTCTTTATGGTGATATGAGTTTGTATATTAGGCCACTTGAGATGTTTCTAAGTGAAGTTGACCATGAAAAATATCCTAATGTTGCTCAAAAATGGAGACTAGAACTTCAAAATATTCCGAGTGTGGCTAAATAA", "aa": "MMRELKIHGIYRHFKGDLYLVEDVATHSETKEKYVVYRALYGDMSLYIRPLEMFLSEVDHEKYPNVAQKWRLELQNIPSVAK", "aa_hexdigest": "72db72f61a8734937b845b8bb826ab75", "start_type": "GTG", "rbs_motif": "TTATT", "psc": { "uniref90_id": "UniRef90_A0A660MWX8", "query_cov": 0.9878048780487805, "subject_cov": 1.0, "identity": 0.914, "score": 153.0, "evalue": 3.01e-47, "valid": true, "product": "DUF1653 domain-containing protein", "uniref50_id": "UniRef50_K9DMN5" }, "pscc": { "uniref50_id": "UniRef50_K9DMN5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_K9DMN5" ], "product": "DUF1653 domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_801", "locus": "GCA003260355_00765" }, { "type": "cds", "sequence": "contig_1", "start": 411694, "stop": 411870, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTCTTGATACGGTTTCTGAATATGATAATGAGAAAAAACATTATCTCCTTGATCTCCGAGAGAGGACGATTTTGGCGATTGCTAATCAATTTGATCATAAAAAAATTCTGACTTTTCTCGGAAAGGTTGGGATTATAGATATTGATGAAACGAAAAAAGAAGTCGTGATCTGA", "aa": "MLLDTVSEYDNEKKHYLLDLRERTILAIANQFDHKKILTFLGKVGIIDIDETKKEVVI", "aa_hexdigest": "a80b95c50e8e1bc8013866957f9e1e03", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6787.8295, "isoelectric_point": 5.543416023254394 }, "id": "NKOCPLAKPK_802", "locus": "GCA003260355_00766" }, { "type": "cds", "sequence": "contig_1", "start": 411937, "stop": 413175, "strand": "+", "frame": 1, "gene": "dnaA", "product": "chromosomal replication initiator protein DnaA", "db_xrefs": [ "GO:0003677", "GO:0003688", "GO:0005524", "GO:0006270", "GO:0006275", "KEGG:K02313", "SO:0001217", "UniRef:UniRef50_A0A2M7XJ83", "UniRef:UniRef90_A0A2W4KES2" ], "nt": "ATGGCGACTTATAATTCGCAGTTTGGGGTCAGATTTGTGGTCTATCCTCCTTTTGCGAATGCTCAGCACGAGCTTCTTACTGATCTCAAAAAATTGCTCAATATCCAAGAAAGCAAAGCTTCCAAAGCTCCTCAACTTGAAAAAAATCTGAAATCAGAGTTAGGTAATTATTTTGGGATTCTCTTTGAGCCGAGTTATCGTTTTGATACTTTTGTTGCTGGGGCGAATAATCAGTTTGCCTTTTCTGCTGCGAAAGCGGTGGCTGATGCTCCAGGAACGGCATATAATCCGCTCTTTCTCTATGGGAATGTGGGACTGGGGAAGACGCATCTTATGCAAGCGGTAGGAAATGAAATCATGCAGAACTTCCCTGAAAAAGTCGTGATTTATCTGCCAGCTACCAAGCTCGTAGAGGAGATTGTTCAGGCACTGAGGATGAATAAAATGGCAAATCTGATGAAAAAGTTTGATGAGGTGGATGTGCTTTTGATTGATGATATTCAGTTTCTCGCGGACAAGGACAAGACGCAGGAGGTTTTTCACAATCTCTTCAACGATTTTCATATGAAGAAAAAGCAGGTGATTATCTCCTCCGATCGCCCTCCAAAAGAGCTTCTCCATATAGAGCCAAGACTCAAAAGCAGATTCGCACTGGGGTTGGTTGCTGATGTGCAGGCTCCAGATTTTGAGACGAGAATCGCGATCTTGGAGTCAAAACTCCAGTCTAAAGGGATCAGTATTGATTTTGAATATTTGTCCTTGATTGCGCAGTATATCAAGAGTAATGTGAGGGAGCTGGAAGGGGCTTTGAATATTTTGATCACGAGGCAGCAGCTTTCCAAGGCGGAAATTAGTGAGGAAGATGTGTATAGCTGCCTGAAAACGCTCGGCTATTACTGTGATGAAAATCAGATTAACTTTGAGCAAGTCATGCAGCAGAATAAAAAATCCAGTAAAAATTTTTCTACGCTCGTAGAGATGGTTGCCCAGTATTATTCGCTTTCTATCGCAGAACTCAAATCTGACAGCAGGAAAAAGGAAATCACCCAGGCGAGGCAGATTTTGATGCTCCTTGCCAAGAAATATTTCAATCGGACCTTGGAAAAGATCGGTGATTATTTCGGTGGAAAGAACCACGCAACCGCGATCTATGCGATCAATACGATAGAAAAAAAACTGAAGTCTGATTCTGAGATTCAGCATGATTACAGGGTTTTTGTGGATTGGATTGAGCAATAA", "aa": "MATYNSQFGVRFVVYPPFANAQHELLTDLKKLLNIQESKASKAPQLEKNLKSELGNYFGILFEPSYRFDTFVAGANNQFAFSAAKAVADAPGTAYNPLFLYGNVGLGKTHLMQAVGNEIMQNFPEKVVIYLPATKLVEEIVQALRMNKMANLMKKFDEVDVLLIDDIQFLADKDKTQEVFHNLFNDFHMKKKQVIISSDRPPKELLHIEPRLKSRFALGLVADVQAPDFETRIAILESKLQSKGISIDFEYLSLIAQYIKSNVRELEGALNILITRQQLSKAEISEEDVYSCLKTLGYYCDENQINFEQVMQQNKKSSKNFSTLVEMVAQYYSLSIAELKSDSRKKEITQARQILMLLAKKYFNRTLEKIGDYFGGKNHATAIYAINTIEKKLKSDSEIQHDYRVFVDWIEQ", "aa_hexdigest": "e49111b2353c6b5c2dd3744fd9e8394e", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A2W4KES2", "query_cov": 0.9975728155339806, "subject_cov": 0.8336713995943205, "identity": 0.961, "score": 773.0, "evalue": 2.74e-280, "valid": true, "gene": "dnaA", "product": "chromosomal replication initiator protein DnaA", "uniref50_id": "UniRef50_A0A2M7XJ83", "kegg_orthology_id": "K02313", "go_ids": [ "GO:0003677", "GO:0003688", "GO:0005524", "GO:0006270", "GO:0006275" ] }, "pscc": { "uniref50_id": "UniRef50_A0A2M7XJ83", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2M7XJ83" ], "product": "Chromosomal replication initiator protein DnaA" }, "genes": [ "dnaA" ], "id": "NKOCPLAKPK_803", "locus": "GCA003260355_00767" }, { "type": "cds", "sequence": "contig_1", "start": 413239, "stop": 413688, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBF8944", "UniRef:UniRef100_A0A328EJZ3", "UniRef:UniRef50_A0A660MUW3", "UniRef:UniRef90_A0A328EJZ3" ], "nt": "ATGAACGACATTGAAAGTATTTATAAAAAATTGGGGGGAGAGTCAAAACCAATCTCAGGAGGTGAAGCATACAAAGCCTATAAAGAGGAGAAATTCAAACTACGGCTAAGGATATGTCTCACACTCCATAGCAAGGGTTACAATTGGAATGACCTCGTAATCAAAATCGGTCAACTTGATTCTGAAGAACGAGAGCGCCTTTTGCAGTCTACCAGCCCTAGAGAGATATTGCAACTCTTTGTAAAATGGAAAGAAGGGAAGGTCTCCTTTGATATAGAGGGAGATGTAATAAAGTTTGGTGTTTCTCACGTGGAGCAGTATGAATTTCGAGAGACACTAAGCTGTAAGAGACCTCGGAGACTTCGTCGCCTAGAAATCCTCGAAAAGGAAAACGAACTCAGTCTGAAAGTCTTGGAAGATATGGTCAGAAAAGGTATCATCTGTCTCTAA", "aa": "MNDIESIYKKLGGESKPISGGEAYKAYKEEKFKLRLRICLTLHSKGYNWNDLVIKIGQLDSEERERLLQSTSPREILQLFVKWKEGKVSFDIEGDVIKFGVSHVEQYEFRETLSCKRPRRLRRLEILEKENELSLKVLEDMVRKGIICL", "aa_hexdigest": "f1560f23f44ce98a0580995c8764bdd4", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000DBF8944", "uniref100_id": "UniRef100_A0A328EJZ3", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBF8944", "UniRef:UniRef100_A0A328EJZ3" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EJZ3", "uniref90_id": "UniRef90_A0A328EJZ3" }, "psc": { "uniref90_id": "UniRef90_A0A328EJZ3", "uniref50_id": "UniRef50_A0A660MUW3" }, "pscc": { "uniref50_id": "UniRef50_A0A660MUW3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MUW3" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17520.154500000008, "isoelectric_point": 8.911223030090333 }, "id": "NKOCPLAKPK_804", "locus": "GCA003260355_00768" }, { "type": "cds", "sequence": "contig_1", "start": 413757, "stop": 414602, "strand": "-", "frame": 2, "gene": "trpS", "product": "tryptophan--tRNA ligase", "db_xrefs": [ "EC:6.1.1.2", "GO:0000166", "GO:0004830", "GO:0005524", "GO:0006436", "SO:0001217", "UniRef:UniRef50_A0A7W4EBB0", "UniRef:UniRef90_A0A328EGH7" ], "nt": "ATGCACGGTTTTACACAGACGCAAGAGGCGGAAATTTTGAAAAAAAATAGTATGACGATCGTAAAGCTCTATCTTGCTTGTGGTGCTGATATGGCAAAAACCCTGATTTACAATCCAGCAGAAATTCCTGGACATGCACAACTCAACTGGGCTTTAACCTGCTTGACGATTATGGGAACAATGGAGAGAATGCACAGCTACAAAGACGCTTTGGCAAAGGGAAAATCTGGAGAAATCAGCGTTGGAACTTTTTGCTATCCGATTTTGATGGCAGCGGATATTCTGCTCTATGATGCGGATTTTGTGCCGGTTGGGAAAGATCAGAAACAACATGTAGAATACGCCAGAGATATTGCTCAGAAATTCAATACTCGCTATGGGGAAACCTTCAAAATACCAACTCCTCTGATTAGAGATGAGGTCGCAACCGTAATGGGAACCGATGGAAGAAAAATGTCCAAATCTTACAACAACTATATTGGACTGCTTGAAGAAGAAAACAGTATGCTCAAAAAAATAAAGCAGATTCCAACCAACGCAAAAACCGTTGAAGAACCGAAAAATCCTGATGAATGCAATGTTTATCAGCTTACCAAGCTCTTTTTGACACCAGAAGAAGATCAAGAATTAAGGAGAAAATATGAAGCAGGAGGACTCTCCTATAAAGTAGCAAAGGACTACCTTTTTGAAAAAATGAGGGAAACCTTAGAGCCGATCCAAGCGAAATTCAAGGAGATCTCTGAAGATGAGGTCAGTAAAATGCTTCACGAACACTCAGAAAAAGCAAATGCTATCGCAGAGAAAAAGATTCAAGAAGTATATAAAAGAATTGGGTTTTTTCTCTAA", "aa": "MHGFTQTQEAEILKKNSMTIVKLYLACGADMAKTLIYNPAEIPGHAQLNWALTCLTIMGTMERMHSYKDALAKGKSGEISVGTFCYPILMAADILLYDADFVPVGKDQKQHVEYARDIAQKFNTRYGETFKIPTPLIRDEVATVMGTDGRKMSKSYNNYIGLLEEENSMLKKIKQIPTNAKTVEEPKNPDECNVYQLTKLFLTPEEDQELRRKYEAGGLSYKVAKDYLFEKMRETLEPIQAKFKEISEDEVSKMLHEHSEKANAIAEKKIQEVYKRIGFFL", "aa_hexdigest": "7be5e48a89d4e20ceb755b289c6a2022", "start_type": "ATG", "rbs_motif": "TTCTT", "psc": { "uniref90_id": "UniRef90_A0A328EGH7", "query_cov": 1.0, "subject_cov": 0.8619631901840491, "identity": 0.94, "score": 526.0, "evalue": 1.85e-187, "valid": true, "gene": "trpS", "product": "tryptophan--tRNA ligase", "ec_ids": [ "6.1.1.2" ], "uniref50_id": "UniRef50_A0A7W4EBB0", "go_ids": [ "GO:0000166", "GO:0004830", "GO:0005524", "GO:0006436" ] }, "pscc": { "uniref50_id": "UniRef50_A0A7W4EBB0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7W4EBB0" ], "product": "Tryptophan--tRNA ligase" }, "genes": [ "trpS" ], "id": "NKOCPLAKPK_805", "locus": "GCA003260355_00769" }, { "type": "cds", "sequence": "contig_1", "start": 414968, "stop": 415681, "strand": "+", "frame": 2, "gene": "dnaN", "product": "DNA polymerase III subunit beta", "db_xrefs": [ "EC:2.7.7.7", "GO:0003677", "GO:0003887", "GO:0006260", "GO:0009360", "KEGG:K02338", "SO:0001217", "UniRef:UniRef50_A0A7W4E2V4", "UniRef:UniRef90_A0A660MQU7" ], "nt": "GTGATGAAAATTAGTTTGGAACTTCCAAAGGTTCTTGGAATGCTCGATATTGCTGCGAGATTTGTTTCTAAAAATTCAACGCTTCCGATCCTTCAGAATATCTATCTTAAGGCATCAATTGATACTTTGCTAATTAGAGCAACGGATATGGAAAAATATGTTGAACTTGAGCTTCCTTGTGCGGTTCAGATTGAAGGAGCTGTAACGGTTAATGCGAAGACTTTCCTTGATATTCTTAAGACGATTGATAGTGAAACGGTGGAGTTTTCTGTTGATCAAAAGACAACAGTAATGACAATTAAAACTTCAAAAGACACTTTTGATATTAATGGAATCCCCGCTTCTGAATATGTTGCTCTTCCAGAAGTTCCTCAGGACAATACTTTAATGCTAGATACGATGATTTTTTCAAAAGGAATCGAGAAAGTTGAGTATGCTATTACAGAAAAGAGCTTTTCACCTGTTTTGACGGGAGTCCTTATGAAATCAAAAACGGAAGACGCACAGCACAAAATTATTTTTGTGGGAACGGATAGTTTTAGGCTCTCTGAATATAAGGTTACAAGTTCTCTTTCTCAGGATTTTTCTCTGATTATCCCTAAAGTTTCTATTAGTGATATTAAATATTTGGTGAACTTTGCTATTGATAAGGAAGTTCCAGAAATGACAGTTAAGTATTCAGATAATTTGATTGCTTTTGAACTTACTTTGTGA", "aa": "MMKISLELPKVLGMLDIAARFVSKNSTLPILQNIYLKASIDTLLIRATDMEKYVELELPCAVQIEGAVTVNAKTFLDILKTIDSETVEFSVDQKTTVMTIKTSKDTFDINGIPASEYVALPEVPQDNTLMLDTMIFSKGIEKVEYAITEKSFSPVLTGVLMKSKTEDAQHKIIFVGTDSFRLSEYKVTSSLSQDFSLIIPKVSISDIKYLVNFAIDKEVPEMTVKYSDNLIAFELTL", "aa_hexdigest": "1a6f8e800c051a9426958a580d5e3b7f", "start_type": "GTG", "rbs_motif": "TTATT", "genes": [ "dnaN" ], "truncated": "3-prime", "pseudogene": { "start": 414971, "stop": 415984, "observations": { "mutation": [ 415679, 415820 ], "stop": [ 415679, 415820 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A660MQU7", "query_cov": 0.9957805907172996, "subject_cov": 0.6210526315789474, "identity": 1.0, "score": 441.0, "evalue": 4.75e-154, "gene_start": 2, "gene_end": 237, "reference_start": 1, "reference_end": 236, "reference_sequence": "MKISLELPKVLGMLDIAARFVSKNSTLPILQNIYLKASIDTLLIRATDMEKYVELELPCAVQIEGAVTVNAKTFLDILKTIDSETVEFSVDQKTTVMTIKTSKDTFDINGIPASEYVALPEVPQDNTLMLDTMIFSKGIEKVEYAITEKSFSPVLTGVLMKSKTEDAQHKIIFVGTDSFRLSEYKVTSSLSQDFSLIIPKVSISDIKYLVNFAIDKEVPEMTVKYSDNLIAFELTLGEVRIVATSLLIQGNFPEYEREEVMPTQFSTKVMLDKRDCENAIKKIGILTKDINNFVQIETQATKVIISSGKTDKGAGTTEIPAIIDGEALTFAVNGRYVMDFIRMMSSDTLIFNIVNGQKPLVLMDKDDPSNRYVVRPLTNI" }, "paralog": false, "identity": 0.9940652818991098, "score": 637.0, "evalue": 2.06e-233, "description": "Internal stop codon at 415679, 415820. Nonsense mutation around 415679, 415820.", "psc": { "uniref90_id": "UniRef90_A0A660MQU7", "gene": "dnaN", "product": "DNA polymerase III subunit beta", "ec_ids": [ "2.7.7.7" ], "uniref50_id": "UniRef50_A0A7W4E2V4", "kegg_orthology_id": "K02338", "go_ids": [ "GO:0003677", "GO:0003887", "GO:0006260", "GO:0009360" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A660MQU7", "EC:2.7.7.7", "UniRef:UniRef50_A0A7W4E2V4", "KEGG:K02338", "GO:0003677", "GO:0003887", "GO:0006260", "GO:0009360" ] }, "pscc": { "uniref50_id": "UniRef50_A0A7W4E2V4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7W4E2V4" ], "product": "Beta sliding clamp" } }, "id": "NKOCPLAKPK_806", "locus": "GCA003260355_00770" }, { "type": "cds", "sequence": "contig_1", "start": 415826, "stop": 416113, "strand": "+", "frame": 2, "gene": null, "product": "DNA polymerase III beta sliding clamp C-terminal domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A948C4K3" ], "nt": "TTGACTAAGGATATCAATAATTTTGTTCAGATTGAAACTCAGGCGACCAAAGTTATTATTTCTTCAGGAAAGACAGATAAAGGAGCGGGAACTACTGAGATTCCTGCAATTATAGATGGAGAAGCACTTACTTTCGCTGTAAATGGAAGATATGTGATGGATTTTATTAGAATGATGTCTTCTGATACCTTGATCTTTAATATTGTGAATGGGCAGAAACCTTTGGTATTAATGGATAAGGACGATCCTTCTAATAGATATGTTGTGAGACCACTTACTAACATTTAA", "aa": "MTKDINNFVQIETQATKVIISSGKTDKGAGTTEIPAIIDGEALTFAVNGRYVMDFIRMMSSDTLIFNIVNGQKPLVLMDKDDPSNRYVVRPLTNI", "aa_hexdigest": "7f6666cb3894ac929f6a3db44b9e136f", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A948C4K3", "query_cov": 0.9894736842105263, "subject_cov": 0.9791666666666666, "identity": 0.585, "score": 125.0, "evalue": 1.54e-35, "valid": false, "product": "DNA polymerase III beta sliding clamp C-terminal domain-containing protein", "uniref50_id": "UniRef50_A0A948C4K3" }, "pscc": { "uniref50_id": "UniRef50_A0A948C4K3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A948C4K3" ], "product": "DNA polymerase III beta sliding clamp C-terminal domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_807", "locus": "GCA003260355_00771" }, { "type": "cds", "sequence": "contig_1", "start": 416130, "stop": 416333, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF07963.17" ], "nt": "ATGTTTAGTCAGAAAAAAAAAGGATTTACTTTGTTTGAGCTTCTTATTGTTGTTTTTGTGCTTGCGATTTTGATAGGGAGTGGAATTTATGGGATTTCTACTGCTGGAGCAGACAAGTTTTATGCTGAAAGCTGCGTGAATACTTTATATGGTCCGGTTTCCAACTGGATTTATTCCGCTTCGGTTGGTAAAGTTATTTCGTGA", "aa": "MFSQKKKGFTLFELLIVVFVLAILIGSGIYGISTAGADKFYAESCVNTLYGPVSNWIYSASVGKVIS", "aa_hexdigest": "7f1289c07542efb162e2ca5d3b2f030b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07963.17", "name": "N_methyl", "length": 23, "aa_cov": 0.34328358208955223, "hmm_cov": 1.0, "evalue": 6.141448728946594e-09, "score": 31.636716842651367, "start": 5, "stop": 27 } ], "seq_stats": { "molecular_weight": 7214.396900000003, "isoelectric_point": 8.85242786407471 }, "id": "NKOCPLAKPK_808", "locus": "GCA003260355_00772" }, { "type": "cds", "sequence": "contig_1", "start": 416863, "stop": 416976, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAACAGACGATTCTTCGTATTTAGAGGAAAGTATCAAAGATCAGGCTTTTTCTTGGGATGAAAAAGGGGAATTGATTCAGGATTTTTTGGCTTTTATCAAGAAATGGTGA", "aa": "MKTDDSSYLEESIKDQAFSWDEKGELIQDFLAFIKKW", "aa_hexdigest": "958e4a86468b4293c6e0a0c1c96b39c7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4442.904399999999, "isoelectric_point": 4.356504249572754 }, "id": "NKOCPLAKPK_809", "locus": "GCA003260355_00773" }, { "type": "cds", "sequence": "contig_1", "start": 417127, "stop": 417450, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTGGTGTTTTGGTATTAAAAGGACGAAAAAATGTCAATTTAAAAGATTTTGTTTGAGTTTTTTCGTTGATGCGGATCATCTTGGTCTTGAGCATTTGCTTTTAGGTGAACAAGATATTTATCTTCCTTATTGGGTTGCACATTTGGTGCCTCTTTATCAGAGAGTGAGGAGTGAGCGTTTTTTTCAAGTGAACTCTCGGATTAGACGCTTTTTACCGAATGCCCCTTTGAAACAGGCTATCTTTCTTGGAAAGTTGAAGGTATTTGAGGGGAAGGGGCGATTTAGGCGTTTAATAGAATGTATTTTTATGGGGAAGCTTTGA", "aa": "MWCFGIKRTKKCQFKRFCLSFFVDADHLGLEHLLLGEQDIYLPYWVAHLVPLYQRVRSERFFQVNSRIRRFLPNAPLKQAIFLGKLKVFEGKGRFRRLIECIFMGKL", "aa_hexdigest": "6ff09a9ac15240723a629e92c3e0ee34", "start_type": "ATG", "rbs_motif": "TTTTTA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12869.261499999982, "isoelectric_point": 10.161329460144039 }, "id": "NKOCPLAKPK_810", "locus": "GCA003260355_00774" }, { "type": "cds", "sequence": "contig_1", "start": 417638, "stop": 417913, "strand": "-", "frame": 3, "gene": null, "product": "DNA topoisomerase (ATP-hydrolyzing)", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2H6B788" ], "nt": "GTGTATGTTTACAATGAAGAGCATACGCCGGACATGTACGGATTTAATGCTGATGATAGTGTAGAAATCCAGAGATATAAAGGTCTCGGAGAAATGAATGCAGAACAGCTCTGGGAAACTACGATGGATCCAGCGACGAGGCAACTCAAACAAATTACCGTAGAAGATGCAGAAGAAACCGATAGACTTTTCAGAATCCTGATGGGAGAAGATGTACAATCCAGAAAGCACTTCATCCTCACCAATGCAAAATCAGTAAAAGGATTGGATGTGTAA", "aa": "MYVYNEEHTPDMYGFNADDSVEIQRYKGLGEMNAEQLWETTMDPATRQLKQITVEDAEETDRLFRILMGEDVQSRKHFILTNAKSVKGLDV", "aa_hexdigest": "5109f564a909204cb4f7f358650ea76b", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A2H6B788", "query_cov": 0.8241758241758241, "subject_cov": 0.9493670886075949, "identity": 0.547, "score": 85.1, "evalue": 5.58e-20, "valid": false, "product": "DNA topoisomerase (ATP-hydrolyzing)", "uniref50_id": "UniRef50_A0A2H6B788" }, "pscc": { "uniref50_id": "UniRef50_A0A2H6B788", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2H6B788" ], "product": "DNA topoisomerase (ATP-hydrolyzing)" }, "genes": [], "id": "NKOCPLAKPK_811", "locus": "GCA003260355_00775" }, { "type": "cds", "sequence": "contig_1", "start": 417968, "stop": 418159, "strand": "-", "frame": 3, "gene": null, "product": "DNA topoisomerase (ATP-hydrolyzing)", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A920P6D0" ], "nt": "ATGATGGCAAATAATGAGGTAAAAGCACTCATTACCGCGATTGGCTGCGGAATCAAGGATAGCTTTGATATTGAAGGACTCAGATACGAAAAAATCATCATCATGACCGATGCCGATGTTGATGGTGCCCATATTAGAACGCTACTTTTGACCTTCTTCTTTAGGTTTATGAGACCATTAGTTGAAGGCTGA", "aa": "MMANNEVKALITAIGCGIKDSFDIEGLRYEKIIIMTDADVDGAHIRTLLLTFFFRFMRPLVEG", "aa_hexdigest": "1d45a48061b94ec497a6725edead6544", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A920P6D0", "query_cov": 0.9206349206349206, "subject_cov": 0.9666666666666667, "identity": 0.69, "score": 87.0, "evalue": 1.92e-21, "valid": false, "product": "DNA topoisomerase (ATP-hydrolyzing)", "uniref50_id": "UniRef50_A0A920P6D0" }, "pscc": { "uniref50_id": "UniRef50_A0A920P6D0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A920P6D0" ], "product": "DNA topoisomerase (ATP-hydrolyzing)" }, "genes": [], "id": "NKOCPLAKPK_812", "locus": "GCA003260355_00776" }, { "type": "cds", "sequence": "contig_1", "start": 418253, "stop": 418597, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00204.31" ], "nt": "GTGACCGATGGTCTCTATGGAATCATCACGGTTAAAATCCCTGAACCTCAATTTGAAGGACAGACCAAAGGAAGACTCGGAAATAGCTATGTTCGTGGAGCAGTTGAAGAGCTGACTTACAACTATCTCAAAGACTATTTTGCAGAAAATCCAGAAGAATTTGATAAAATCTTCGCAAAAATTGACCTCGCTGCGAGAGCCAGACTTGCTGCAAAATTTGCAAAAGAAACGGTACTCAGAAAAAATGTACTTGCTGGTGGCGTGCTTCCAGGAAAACTTGCTGACTGCAGCAAAAGAGGAAAACATGGAACGGAGCTTTACATTGTGGAGGGAGATTCTGCGTGA", "aa": "MTDGLYGIITVKIPEPQFEGQTKGRLGNSYVRGAVEELTYNYLKDYFAENPEEFDKIFAKIDLAARARLAAKFAKETVLRKNVLAGGVLPGKLADCSKRGKHGTELYIVEGDSA", "aa_hexdigest": "36022eab07c7240fcc3d76054832b622", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00204.31", "name": "DNA_gyraseB", "length": 74, "aa_cov": 0.6491228070175439, "hmm_cov": 1.0, "evalue": 4.47329591402001e-22, "score": 74.80035400390625, "start": 3, "stop": 76 } ], "seq_stats": { "molecular_weight": 12557.207099999981, "isoelectric_point": 7.786958885192871 }, "id": "NKOCPLAKPK_813", "locus": "GCA003260355_00777" }, { "type": "cds", "sequence": "contig_1", "start": 418616, "stop": 418888, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00204.31" ], "nt": "ATGTATGAAGGAGGGATCAAGACTTGGCTTAGGAACCTCGTTGGGAATCAGCAGATTTTGAGTTCTGCTCATTATTTCAAAGCGGAGACCAAAGATTGCCAAGCAGAAATTTCGTTCCAGTTTGTAAATACCAATAACGACCATACCTTATCTTTCGTAAACAATATTCCGACCAGAGATGGAGGAACTCACATTCTCGGCTTCAAATCAGCTTTATTGAAGATTATGAATGAAATTGGGAAAGAAAGAGATAAAATAGATAAAAAAATCTGA", "aa": "MYEGGIKTWLRNLVGNQQILSSAHYFKAETKDCQAEISFQFVNTNNDHTLSFVNNIPTRDGGTHILGFKSALLKIMNEIGKERDKIDKKI", "aa_hexdigest": "31dd7939d7c12019a64d3fab1e8c6fc2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00204.31", "name": "DNA_gyraseB", "length": 88, "aa_cov": 0.9444444444444444, "hmm_cov": 1.0, "evalue": 2.095167984769439e-21, "score": 72.61714935302734, "start": 5, "stop": 89 } ], "seq_stats": { "molecular_weight": 10242.556199999992, "isoelectric_point": 8.789377784729005 }, "id": "NKOCPLAKPK_814", "locus": "GCA003260355_00778" }, { "type": "cds", "sequence": "contig_1", "start": 418904, "stop": 419026, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCTGATGGGACAATTTTTGAGACCCTTGAATTTAGTGAAACCGCGGAAATTGCGAGAATGAAGCAAGCAGCTTACCTCACTCCAGGGGTAAGTTTTACCTTCAAAAATATGAAAACCTGA", "aa": "MPDGTIFETLEFSETAEIARMKQAAYLTPGVSFTFKNMKT", "aa_hexdigest": "b853986446c6b8a0946117d3a0352b63", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4503.1335, "isoelectric_point": 5.002479362487793 }, "id": "NKOCPLAKPK_815", "locus": "GCA003260355_00779" }, { "type": "cds", "sequence": "contig_1", "start": 419042, "stop": 419545, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02518.31" ], "nt": "ATGACCACGCCAGAAAACAATCATGAATACGATGCCTCACAGATTAGAGTGCTTGAGGGGCTAGAACCAGTAAGACAAAGACCTGGAATGTATATCGGAAGCACGGATTATAGAGGGCTTTACCATATGATACAGGAAATCGTAGATAATGCAGTAGATGAGGCACTTGCGGGCTACTGTAAAGATGTAACTTCTATTCTCAACGAAGATGGTTCTGTGACAGTGCACGATAATGGACGTGGAATCCCGGTTGATATCCATCCAAAGACTGGAAAATCAGCCTTAGAAACCGTTTATACTGTGCTTCATGCCGGAGGGAAATTTGACAAAGGAGCCTACAAGATTTCTGGAGGACTTCACGGAGTGGGAGCTTCTGTAGTAAACGCACTTTCCACCTGGCTTGAAGTGACCGTTTACAAGAACGGGAAAATCCACAAAATGAGATTTGAAAAAGGAGTTCCTCAGGGAGAACTTCAAATCATTGGAGAAACTGACAAAACCTGA", "aa": "MTTPENNHEYDASQIRVLEGLEPVRQRPGMYIGSTDYRGLYHMIQEIVDNAVDEALAGYCKDVTSILNEDGSVTVHDNGRGIPVDIHPKTGKSALETVYTVLHAGGKFDKGAYKISGGLHGVGASVVNALSTWLEVTVYKNGKIHKMRFEKGVPQGELQIIGETDKT", "aa_hexdigest": "59151ae6ff2b0a21d1a5fbe1bd52e8f6", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0034A35755", "query_cov": 0.9640718562874252, "subject_cov": 0.8009950248756219, "identity": 0.652, "score": 213.0, "evalue": 7.2e-68, "valid": false, "product": "ATP-binding protein", "uniref50_id": "UniRef50_A0A7S3EN75" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02518.31", "name": "HATPase_c", "length": 106, "aa_cov": 0.6167664670658682, "hmm_cov": 0.8490566037735849, "evalue": 5.273427160744939e-18, "score": 61.97660827636719, "start": 36, "stop": 138 } ], "seq_stats": { "molecular_weight": 18227.322799999998, "isoelectric_point": 5.772477149963378 }, "id": "NKOCPLAKPK_816", "locus": "GCA003260355_00780" }, { "type": "cds", "sequence": "contig_1", "start": 419808, "stop": 419969, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGATTGTAGTTGCCGTGATTGGAATTTTGACGGCAGTTGGAATGGGGCTCAGTCGGAGTAGCTTGGAAAAACTTAAAGTAAAAGCTGTAAATGAAGAATTTGCCGGTTTTTTTGATACGTTATTTTTGCAGGTAAATGCTTCAAACTATTATCAGTGA", "aa": "MLIVVAVIGILTAVGMGLSRSSLEKLKVKAVNEEFAGFFDTLFLQVNASNYYQ", "aa_hexdigest": "567af20580f1a5d4cae93d7ca29667a1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5796.751800000001, "isoelectric_point": 6.013360786437987 }, "id": "NKOCPLAKPK_817", "locus": "GCA003260355_00781" }, { "type": "cds", "sequence": "contig_1", "start": 420447, "stop": 420776, "strand": "+", "frame": 3, "gene": null, "product": "HIT domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A288DJE3", "UniRef:UniRef90_A0A328EGK4" ], "nt": "GTGATCCCTAAAGAGCATTTTGACTCAGATTTTTTCTTAATTGAAGATTCGGACTTTGCTCAGCGCTATTTTTTGGCGATTCGTGAAGTTGTTGAACTTTTAAAGTCGAAATTAAATGTGGTGAGAGTCGGGATGATTATGGAAGGAATGGGCGTAAATCATTTGCATATTAAGCTCTATCCAATGTGGGGACTTTCTGAACAGCGAGAGCCTGAAGTTAGTCCTGAAAAGGTTTTCTTTGATCGCTATCCTGGATTTTTGACTACTAAAGTGGGGGATATGGCTTCGCAGGATGAATTAGCAAAACTTGCGGATATTTTCAAGGTATAA", "aa": "MIPKEHFDSDFFLIEDSDFAQRYFLAIREVVELLKSKLNVVRVGMIMEGMGVNHLHIKLYPMWGLSEQREPEVSPEKVFFDRYPGFLTTKVGDMASQDELAKLADIFKV", "aa_hexdigest": "b1461bcd07697f4a9f770b1d576d6d82", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 420321, "stop": 420773, "observations": { "mutation": [ 420360 ], "stop": [ 420360 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EGK4", "query_cov": 0.9908256880733946, "subject_cov": 0.7152317880794702, "identity": 0.963, "score": 207.0, "evalue": 3.75e-67, "gene_start": 1, "gene_end": 108, "reference_start": 43, "reference_end": 150, "reference_sequence": "MQKSDCLFCKIVAGEIPSYKVWENEEFYAFLDLFPNCKGQTLVIPKEHFDSDFFLIEDSDFAQRYFLAIREVVELLKSKLNVVRVGMIMEGMGVNHLHIKLYPMWGLSEQREPEVSSEKVFFDRYLGFLTTKVGDMASQDELAKLSDIFKA" }, "paralog": false, "identity": 0.9666666666666667, "score": 296.0, "evalue": 1.29e-104, "description": "Internal stop codon at 420360. Nonsense mutation around 420360.", "psc": { "uniref90_id": "UniRef90_A0A328EGK4", "product": "HIT domain-containing protein", "uniref50_id": "UniRef50_A0A288DJE3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EGK4", "UniRef:UniRef50_A0A288DJE3" ] }, "pscc": { "uniref50_id": "UniRef50_A0A288DJE3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A288DJE3" ], "product": "HIT domain-containing protein" } }, "id": "NKOCPLAKPK_818", "locus": "GCA003260355_00782" }, { "type": "cds", "sequence": "contig_1", "start": 420932, "stop": 421096, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF20772.2" ], "nt": "ATGTATAGTATTATTGGAAAGAAAATTCAAATTGCAGCCAAAAATGGAGCAGATCCAAAGATGAATCCTTCATTGGAGATGATGCTTGAAAAGGCTCGTTATCATGGATTGCCAAAAGATGTTGTGGAAAGGGCGATCTTGAAAGGTTCTGGGCAACTGGAATGA", "aa": "MYSIIGKKIQIAAKNGADPKMNPSLEMMLEKARYHGLPKDVVERAILKGSGQLE", "aa_hexdigest": "2f6443b156af75857ed595f0305b518b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF20772.2", "name": "TACO1_YebC_N", "length": 54, "aa_cov": 0.9629629629629629, "hmm_cov": 1.0, "evalue": 1.5824519534085034e-17, "score": 60.24361801147461, "start": 1, "stop": 52 } ], "seq_stats": { "molecular_weight": 5970.018400000001, "isoelectric_point": 9.455400657653808 }, "id": "NKOCPLAKPK_819", "locus": "GCA003260355_00783" }, { "type": "cds", "sequence": "contig_1", "start": 421103, "stop": 421222, "strand": "+", "frame": 2, "gene": null, "product": "TACO1/YebC-like second and third domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3D0USK5" ], "nt": "ATGAAGGAAGTTTTTTATGAAGGGTATGGGCCTAATGGTTCTGCGATTTTGATTAAAACAGTAACCTCAAATACGAATAGAACGTCTCAATCACTCAGAACTGCCTTACAAAAAGCTTGA", "aa": "MKEVFYEGYGPNGSAILIKTVTSNTNRTSQSLRTALQKA", "aa_hexdigest": "4a770ce76f5a1278cf324f9e53db4109", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3D0USK5", "query_cov": 0.9743589743589743, "subject_cov": 0.8444444444444444, "identity": 0.6579999999999999, "score": 54.7, "evalue": 3.25e-09, "valid": false, "product": "TACO1/YebC-like second and third domain-containing protein", "uniref50_id": "UniRef50_A0A3D0USK5" }, "pscc": { "uniref50_id": "UniRef50_A0A3D0USK5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3D0USK5" ], "product": "TACO1/YebC-like second and third domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_820", "locus": "GCA003260355_00784" }, { "type": "cds", "sequence": "contig_1", "start": 421229, "stop": 421408, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTGAAATTGGTGCTGTAGCTCGACAATTTAAGGAGCAGGGATTTATCGTGATTGATGGGAAATCACAGATGGTAGAAGATAAAGGAAGGCAGATCGAACAAATCACGCCTTTTGATGCAGAAACATTAGAGATGGAGATGATGGATTTGCCAATTGAAGATTTAAGTATTGACTGA", "aa": "MAEIGAVARQFKEQGFIVIDGKSQMVEDKGRQIEQITPFDAETLEMEMMDLPIEDLSID", "aa_hexdigest": "70d67f7d35e07a778c4902ffce127d39", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6688.565999999997, "isoelectric_point": 4.1120397567749025 }, "id": "NKOCPLAKPK_821", "locus": "GCA003260355_00785" }, { "type": "cds", "sequence": "contig_1", "start": 421643, "stop": 421858, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00482.28" ], "nt": "ATGATCAAAGTTGGGGAAGAAACCGCCCATCTTGAGGAGTCAATGCAAAACATCATTTCAATGTATCAAGAAGAACTCGATAATACAATTACTCAATTCTCAAAAATATTAGAGCCTATCATCTTAATTTTCATGGGGGTTATCGTAGCCCTCGTTGCATCAGGAATTTTTGGAGTTGTATTCTCCATCATGGAAAGTATTGGAAGCACAAACTAG", "aa": "MIKVGEETAHLEESMQNIISMYQEELDNTITQFSKILEPIILIFMGVIVALVASGIFGVVFSIMESIGSTN", "aa_hexdigest": "eb621668a794b40e9fcf9db5579bb392", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00482.28", "name": "T2SSF", "length": 56, "aa_cov": 0.7887323943661971, "hmm_cov": 1.0, "evalue": 1.824199532294976e-10, "score": 37.31044006347656, "start": 1, "stop": 56 } ], "seq_stats": { "molecular_weight": 7822.069699999996, "isoelectric_point": 4.14142551422119 }, "id": "NKOCPLAKPK_822", "locus": "GCA003260355_00786" }, { "type": "cds", "sequence": "contig_1", "start": 421931, "stop": 422299, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCTTCCAGGAATTTTGGAAATGTTTACTGGAGATATGGATAAACTCCCATTAATCACTCGCATCCTAAAATCTACTTCAGATTTCTTTGTCCTTTCCCGAAAAACAATCCTAATAGTACTAGGAACTATTGGACTTATACTCGGAATTTATGGGACAACTGAACAAGGAAAGAAAAACTTCTCTCAAATGCTGATGTCTCTACCTCTGGTAGGAAAAATGACAAAAACCTACTATCTTATCAAACGAGCAAGATATACAAAACTGATGGTTGGATCTGGATTAGACTACGTTCAAACCTTTCGTTTACTAAGAGGTGTTCTAGCAATCCCCGCATACCAAGATATGATTGAAAAAGTAATGGCTTGA", "aa": "MLPGILEMFTGDMDKLPLITRILKSTSDFFVLSRKTILIVLGTIGLILGIYGTTEQGKKNFSQMLMSLPLVGKMTKTYYLIKRARYTKLMVGSGLDYVQTFRLLRGVLAIPAYQDMIEKVMA", "aa_hexdigest": "56edf1ad35fa89bc6c4c4fc22050114a", "start_type": "GTG", "rbs_motif": "TTATT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13745.511499999986, "isoelectric_point": 9.885920524597168 }, "id": "NKOCPLAKPK_823", "locus": "GCA003260355_00787" }, { "type": "cds", "sequence": "contig_1", "start": 422453, "stop": 422710, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00482.28" ], "nt": "ATGCATGGTTTGGATAAAAATTCATTCTCTGCAAAAGATAAAATTTTATTTTACAAAGAAATCGTTTACATGCTACGTGGAGGGATTTCAATCCTCCAAGCGATTGATACGATCAAAAAATCCACAACAAACTACGCACTAAAAACAATCACAACAAGCATTGCCCACCATCTCAATGAGGGAAAGAGTTTTTCTTATGCCATAGGACGTTTACCCGAATATTTCGATGAATCTGATAGTGCAATCATCAAAACCTGA", "aa": "MHGLDKNSFSAKDKILFYKEIVYMLRGGISILQAIDTIKKSTTNYALKTITTSIAHHLNEGKSFSYAIGRLPEYFDESDSAIIKT", "aa_hexdigest": "b6cdbc95046a8e8d6fc5f070c4faf7d6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00482.28", "name": "T2SSF", "length": 61, "aa_cov": 0.7058823529411765, "hmm_cov": 1.0, "evalue": 5.877104618704678e-10, "score": 35.6688346862793, "start": 17, "stop": 76 } ], "seq_stats": { "molecular_weight": 9560.868399999994, "isoelectric_point": 8.892978477478028 }, "id": "NKOCPLAKPK_824", "locus": "GCA003260355_00788" }, { "type": "cds", "sequence": "contig_1", "start": 422790, "stop": 423161, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EK02", "UniRef:UniRef90_A0A328EK02" ], "nt": "ATGGGAAATCTAAATTCGAAAAAAATGAAAATCGACGAAAAGAAAGTTCTCAAAAATCTCGACCAATACCTTATAAAAAATAATCCAAGAGAAAAGGTATCTAGTCCAAACGGAGTCATCGAGGGTATCGAAATAGGAGAACCAGAACCTGTTGAAAAAGATTCTGTATTTATGAAAGTACCAATTCTTATCAAAGTAACGTTTTCTGACAAAGATGATCTGATTTCTTTTGTTGATAACATTGAAAAATTCATCATTTCCAATACCGAAGACAGTATTCTATATAAGATTGAGGAACTTTCCTACGACATGATGGCCTATGATGAAAGTCAGACTACTGATATAGTTTTAACAGCATACTACTTTAACTAA", "aa": "MGNLNSKKMKIDEKKVLKNLDQYLIKNNPREKVSSPNGVIEGIEIGEPEPVEKDSVFMKVPILIKVTFSDKDDLISFVDNIEKFIISNTEDSILYKIEELSYDMMAYDESQTTDIVLTAYYFN", "aa_hexdigest": "191ea964bf4bdf3a950f191581370e62", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 422790, "stop": 423461, "observations": { "mutation": [ 423185, 423419 ], "stop": [ 423185, 423419 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EK02", "query_cov": 1.0, "subject_cov": 0.467680608365019, "identity": 0.9840000000000001, "score": 232.0, "evalue": 4.6e-75, "gene_start": 1, "gene_end": 123, "reference_start": 141, "reference_end": 263, "reference_sequence": "MQEQNPQLINNLLEQQKNTAYIKELSEDTSENQLSITRVVTAKYKVYVIILLILGVLLGTRYLPQAYQYFEATQALEEKKNKELNDLKAEIANLEQSKVQLEDLQNNKDQIIACVNEEKECSSLSEGLKSDFGSVVSYLQMGNLNSKKMKIDEKKVLKNLDQYLIKNNPREKVSSPNGVIEGIEIGEPEPVEKDSVFMKVPILIKVTFSDKDDLISFVDNIEKFIISNTEDSILYKIEELSYDMMAYEESQTTDIVLPAYYFN" }, "paralog": false, "identity": 0.9820627802690582, "score": 423.0, "evalue": 7.35e-153, "description": "Internal stop codon at 423185, 423419. Nonsense mutation around 423185, 423419.", "psc": { "uniref90_id": "UniRef90_A0A328EK02", "uniref50_id": "UniRef50_A0A328EK02", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EK02", "UniRef:UniRef50_A0A328EK02" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EK02", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EK02" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 14165.045899999986, "isoelectric_point": 4.553621864318848 }, "id": "NKOCPLAKPK_825", "locus": "GCA003260355_00789" }, { "type": "cds", "sequence": "contig_1", "start": 423183, "stop": 423413, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTTGGGACAAGATATCTCCCACAGGCCTACCAATATTTTGAGGCAACTCAAGCTCTAGAAGAGAAAAAAAATAAAGAGCTTAATGACCTAAAAGCAGAAATAGCAAACCTGGAACAATCAAAAGTTCAGCTAGAGGATCTGCAAAATAATAAAGATCAAATAATAGCTTGTGTAAACGAAGAAAAGGAATGTTCGTCACTATCAGAAGGTTTAAAGTCTGACTTTTGA", "aa": "MLGTRYLPQAYQYFEATQALEEKKNKELNDLKAEIANLEQSKVQLEDLQNNKDQIIACVNEEKECSSLSEGLKSDF", "aa_hexdigest": "f12504936b7cbed79db3cde516cfa7df", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8727.6423, "isoelectric_point": 4.539923667907716 }, "id": "NKOCPLAKPK_826", "locus": "GCA003260355_00790" }, { "type": "cds", "sequence": "contig_1", "start": 423417, "stop": 423581, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAGAACAGAATCCACAATTAATCAATAATCTGCTTGAGCAGCAAAAAAACACAGCATATATAAAAGAACTCTCAGAAGATACCAGTGAAAACCAATTAAGCATTACAAGAGTCGTAACCGCAAAATATAAAGTATATGTCATTATTTTGCTTATTTTATGA", "aa": "MQEQNPQLINNLLEQQKNTAYIKELSEDTSENQLSITRVVTAKYKVYVIILLIL", "aa_hexdigest": "30a4a4ddc0887da293b313011cfc9ff0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6293.2003, "isoelectric_point": 5.1057558059692365 }, "id": "NKOCPLAKPK_827", "locus": "GCA003260355_00791" }, { "type": "cds", "sequence": "contig_1", "start": 423616, "stop": 424011, "strand": "-", "frame": 1, "gene": "pilN", "product": "PilN domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MRR7", "UniRef:UniRef90_A0A660MRR7" ], "nt": "TTGCAAGAGCAAACAATAGCAGCTAAAGAGCAAGAAATCCAGAATGAACAGAAAAAAATCGAAAACTTCTATCAAAATACTGGATTAAAAGAATTTCTCGCTGTGAAAAAACTAGAAACAACAAGAAATCACCTACCACGAAGTGATCACATAGAAAAAATCCTTTCTATCCTATCCAAAGTAAAACACGTAGAAGAAGGAAGAGGAAATATCCTCCTATCTGACTTCAAAATCAACCTTCAAGAACTCTCTCTGAATGGAGTCGTAGGAAACTTAAAAGCCTTATACTTAGCACCTGGAGGATCAGGATTCTCTGTTTTGGACGAATTCAATCAGTTAGAATTCCTCTCCGATATCGCAGTAAGAAATTATGAAAGAAACGAAGATACCAGATGA", "aa": "MQEQTIAAKEQEIQNEQKKIENFYQNTGLKEFLAVKKLETTRNHLPRSDHIEKILSILSKVKHVEEGRGNILLSDFKINLQELSLNGVVGNLKALYLAPGGSGFSVLDEFNQLEFLSDIAVRNYERNEDTR", "aa_hexdigest": "19efb3cfda4ba62388ff8a125097804b", "start_type": "TTG", "rbs_motif": "TTCTA", "genes": [ "pilN" ], "truncated": "both", "pseudogene": { "start": 423550, "stop": 424119, "observations": { "mutation": [ 423618, 424050, 424053 ], "stop": [ 423618, 424050, 424053 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A660MRR7", "query_cov": 1.0, "subject_cov": 0.6931216931216931, "identity": 0.992, "score": 249.0, "evalue": 1.15e-82, "gene_start": 1, "gene_end": 131, "reference_start": 37, "reference_end": 167, "reference_sequence": "MSLSKATKAWKTRYYLLLIIFIGGLGALGYFYGFSYLQEQTIAAKEQEIQNEQKKIENFYQNTGLKEFLAVKKLETTRNHLPRSDHIEKILSILSKVKHVEEGRGNILLSDFKINLQELSLNGVVGNLKALYLAPGGSGFSVLDEFNQLEFLSDIAVRNYERNEDTRGFKFTLSAKVINDARTESTINQ" }, "paralog": false, "identity": 0.9841269841269841, "score": 360.0, "evalue": 8.97e-129, "description": "Internal stop codon at 423618, 424050, 424053. Nonsense mutation around 423618, 424050, 424053.", "psc": { "uniref90_id": "UniRef90_A0A660MRR7", "product": "PilN domain-containing protein", "uniref50_id": "UniRef50_A0A660MRR7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A660MRR7", "UniRef:UniRef50_A0A660MRR7" ] }, "pscc": { "uniref50_id": "UniRef50_A0A660MRR7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MRR7" ], "product": "PilN domain-containing protein" } }, "id": "NKOCPLAKPK_828", "locus": "GCA003260355_00792" }, { "type": "cds", "sequence": "contig_1", "start": 424480, "stop": 424584, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAACCTGCACTTGGACTAAAAAAAGGAAAAATCCTTGATGCAGAAGCCTTTACTACGACGATTAACAAGATTATTGAGCATTTTATCAAGAAGCTAGGGTGA", "aa": "MEPALGLKKGKILDAEAFTTTINKIIEHFIKKLG", "aa_hexdigest": "e836d3f018571c62021bcb03c4af1894", "start_type": "ATG", "rbs_motif": "TTATT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3769.496900000001, "isoelectric_point": 9.303899955749511 }, "id": "NKOCPLAKPK_829", "locus": "GCA003260355_00793" }, { "type": "cds", "sequence": "contig_1", "start": 424627, "stop": 425286, "strand": "+", "frame": 1, "gene": "ftsA", "product": "cell division protein FtsA", "db_xrefs": [ "GO:0005515", "GO:0005524", "GO:0043093", "SO:0001217", "UniRef:UniRef50_A0A288DFW0", "UniRef:UniRef90_A0A660MR30" ], "nt": "ATGCTTGTGCATAGGATTATTGAAGGGAAGAGAATTATGCAAGATGAAGTAGAAAATGATGATATTGATCATCTTTCCAGAATTGTTTCTGAGATTTCAATGAAAAATAATTATGAAACCATTAAAATTGCTCCAGTTTGCTGGATTATTGATGAGGATAAAAGAGAAAAAGATCCTATTGGTCTGAAATGTAAAAAACTTGAATTAATGGCCGATGTCTTTATGCTTCCTAAGAATTTTTATACAGGGCTTGTAGATGCTTTTGATAAGGTTGGACTATCAATCTCAGATATTATCCCTAATATTATTGCTGCTACAGAAGTTGCTATAGATTATGATCATAAAGATCTTGGAACCGTGCTTGTGGATATTGGAAAGAATCAGACTTCTTATGTGATTTATGAGGATGGTTATCCGCTTGGTTATGGGACGATTCCTATGGGAGGAGAAGATGTTACGAAAGATATTTCTATTGGAATGCAGGTAGATATTAAGGAAGCTGAAGAAATCAAGAAAACTCATGGTATGGCGGTTCTTTCTGAGGGGATTAAGTCTGATAGTCAGCTTGATCTAGTCTTTCTCTCTGATATTATTAATGCAAGATATGAGGAAATCTTCCTTAAAGTCAATCAGCATTTAGAAAGAATAGAAAAAGACTGA", "aa": "MLVHRIIEGKRIMQDEVENDDIDHLSRIVSEISMKNNYETIKIAPVCWIIDEDKREKDPIGLKCKKLELMADVFMLPKNFYTGLVDAFDKVGLSISDIIPNIIAATEVAIDYDHKDLGTVLVDIGKNQTSYVIYEDGYPLGYGTIPMGGEDVTKDISIGMQVDIKEAEEIKKTHGMAVLSEGIKSDSQLDLVFLSDIINARYEEIFLKVNQHLERIEKD", "aa_hexdigest": "1518290bd1220c9f70ee6707288bc381", "start_type": "ATG", "rbs_motif": null, "genes": [ "ftsA" ], "truncated": "both", "pseudogene": { "start": 424375, "stop": 425559, "observations": { "mutation": [ 424441, 424582, 425284 ], "stop": [ 424441, 424582, 425284 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A660MR30", "query_cov": 1.0, "subject_cov": 0.5558375634517766, "identity": 1.0, "score": 432.0, "evalue": 1.17e-150, "gene_start": 1, "gene_end": 219, "reference_start": 85, "reference_end": 303, "reference_sequence": "MLMRDIKAVFDIGNDMIKAVVFGKDNEKDIILAKQMEPALGLKKGKILDAEAFTTTINKIIEHFIKKLGGDFIEEVVVGVSHPEMLVHRIIEGKRIMQDEVENDDIDHLSRIVSEISMKNNYETIKIAPVCWIIDEDKREKDPIGLKCKKLELMADVFMLPKNFYTGLVDAFDKVGLSISDIIPNIIAATEVAIDYDHKDLGTVLVDIGKNQTSYVIYEDGYPLGYGTIPMGGEDVTKDISIGMQVDIKEAEEIKKTHGMAVLSEGIKSDSQLDLVFLSDIINARYEEIFLKVNQHLERIEKDGRLPGGILLIGGGSKMMNIDYLAKNVFKLATFFGKDLMVNVGDLSLNIQFVNLLGVYYRSNKYVDISSKKMSFRGAGKAFSKIGNFFKDLF" }, "paralog": false, "identity": 0.9923857868020305, "score": 765.0, "evalue": 3.25e-284, "description": "Internal stop codon at 424441, 424582, 425284. Nonsense mutation around 424441, 424582, 425284.", "psc": { "uniref90_id": "UniRef90_A0A660MR30", "gene": "ftsA", "product": "cell division protein FtsA", "uniref50_id": "UniRef50_A0A288DFW0", "go_ids": [ "GO:0005515", "GO:0005524", "GO:0043093" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A660MR30", "UniRef:UniRef50_A0A288DFW0", "GO:0005515", "GO:0005524", "GO:0043093" ] }, "pscc": { "uniref50_id": "UniRef50_A0A288DFW0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A288DFW0" ], "product": "Cell division protein FtsA" } }, "id": "NKOCPLAKPK_830", "locus": "GCA003260355_00794" }, { "type": "cds", "sequence": "contig_1", "start": 425329, "stop": 425559, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A948C675" ], "nt": "ATGATGAATATTGACTATCTTGCAAAGAATGTTTTCAAACTTGCGACGTTCTTTGGAAAGGATCTCATGGTGAATGTTGGTGATCTGTCCCTTAATATCCAGTTTGTTAATTTACTTGGAGTTTATTATCGATCTAATAAGTATGTTGATATTTCTTCAAAGAAAATGTCCTTTAGAGGAGCTGGAAAAGCTTTTAGTAAGATCGGAAACTTCTTTAAGGATCTCTTTTAG", "aa": "MMNIDYLAKNVFKLATFFGKDLMVNVGDLSLNIQFVNLLGVYYRSNKYVDISSKKMSFRGAGKAFSKIGNFFKDLF", "aa_hexdigest": "1333c13ae9ef8d861893eeb70565bdf0", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A948C675", "query_cov": 1.0, "subject_cov": 1.027027027027027, "identity": 0.539, "score": 82.4, "evalue": 3.16e-19, "valid": false, "uniref50_id": "UniRef50_A0A948C675" }, "pscc": { "uniref50_id": "UniRef50_A0A948C675", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A948C675" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8691.127099999994, "isoelectric_point": 9.7943754196167 }, "id": "NKOCPLAKPK_831", "locus": "GCA003260355_00795" }, { "type": "cds", "sequence": "contig_1", "start": 425619, "stop": 426779, "strand": "+", "frame": 3, "gene": "ftsZ", "product": "cell division protein FtsZ", "db_xrefs": [ "GO:0000910", "GO:0003924", "GO:0005525", "KEGG:K03531", "SO:0001217", "UniRef:UniRef50_A0A7W4E2W2", "UniRef:UniRef90_A0A839JW54" ], "nt": "ATGGTTATCAATGAAATTATGCCTGAGTTTATTCCAGGAGCAAAGATTAAAGTCTTTGGAGTAGGGGGGTGCGGAAATAAAGCCCTCAATAGAATGATCCAAGAAGGATTGACAGGAGTAGAGTTTGTTGCGGTAAATACTGATGCACAGGATCTTGCAACCAACCTTGCACAGCAGAAAGTAAATATCGGACTCAATATTACGAAAGGACTCGGAGCTGGAGCAAACCCTGAAATTGGAAGAAAATCAGCGGAAGAAGATGAAATGAACCTCAAATCAGTAATGCAAGATACTGATATGGTTTTCATTACTGCTGGAATGGGGGGGGGAACTGGAACGGGAGCCGCTCCAGTTGTTGCAAGTATCGCAAAAAGTATGGGAATCCTTACGGTTGGTATTGTTACGAAGCCTTTTAGCTTTGAAGGAAAGAAAAGGTCTATCAATGCTGAAGAAGGACTTAAAAAGATGAAAGAAGTTGTAGATACCTTGATTGTAATTCCAAATGATAAAATTTATACTTTGGTAGACAAAAAAACGACCTTCAAACAAGCCTTTACGATGATTGATAAGGTGCTTTATCTTGGAGTTCAGGGTATTTCTGATTTGATTACCAAGCCTGGAGATATCAATATTGACTTTGCAGATATTAGAGAAGTGATGCTTAATTCTGGAAACGCTTTGCTCGGAATCGGATACGGAGCTGGAGAAAAGAGGGCAGTGGATGCAGCGAGAAAAGCTATTGAAAATCCACTCCTTGAAACCAATCTTGATGGAGCGAAGAAAGTGATTTTTGCGGTATCTGGAGGAATGGATTTGACACCGATTGAAGTGCAGGAAGCTGCTGCGATTGTTGAGGAAATCCTTGATGATGATGTGAATATGATTTGGGGAATGAGCTTTGATGAATCTTTTGAAGACGAAGTAAAGGTTACGATTATTGCGACCGGATTTGAAGAGCAGAGCAAAGAACCTATTTTGAAGCCTGTTCAGAGAAATTTCCTTGGTCAAAAGATTGAAACTCCTAAAAGAGAATCTGAAAATTATATCACGAGAGGACTTAGAAACACTGAAACTAAACCAGAACCAGAAACTGTAAAAGAACCTGAGGAAGATACTGAAGTTCCAGCGTTCTTGAGAAGACAAATGAAACAGCAAGGATAA", "aa": "MVINEIMPEFIPGAKIKVFGVGGCGNKALNRMIQEGLTGVEFVAVNTDAQDLATNLAQQKVNIGLNITKGLGAGANPEIGRKSAEEDEMNLKSVMQDTDMVFITAGMGGGTGTGAAPVVASIAKSMGILTVGIVTKPFSFEGKKRSINAEEGLKKMKEVVDTLIVIPNDKIYTLVDKKTTFKQAFTMIDKVLYLGVQGISDLITKPGDINIDFADIREVMLNSGNALLGIGYGAGEKRAVDAARKAIENPLLETNLDGAKKVIFAVSGGMDLTPIEVQEAAAIVEEILDDDVNMIWGMSFDESFEDEVKVTIIATGFEEQSKEPILKPVQRNFLGQKIETPKRESENYITRGLRNTETKPEPETVKEPEEDTEVPAFLRRQMKQQG", "aa_hexdigest": "350189804704ebc2ee6cc7594d39fc42", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A839JW54", "query_cov": 1.0025906735751295, "subject_cov": 1.0051948051948052, "identity": 0.925, "score": 676.0, "evalue": 4.22e-244, "valid": true, "gene": "ftsZ", "product": "cell division protein FtsZ", "uniref50_id": "UniRef50_A0A7W4E2W2", "kegg_orthology_id": "K03531", "go_ids": [ "GO:0000910", "GO:0003924", "GO:0005525" ] }, "pscc": { "uniref50_id": "UniRef50_A0A7W4E2W2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7W4E2W2" ], "product": "Cell division protein FtsZ" }, "genes": [ "ftsZ" ], "id": "NKOCPLAKPK_832", "locus": "GCA003260355_00796" }, { "type": "cds", "sequence": "contig_1", "start": 426863, "stop": 427369, "strand": "+", "frame": 2, "gene": null, "product": "ATP synthase epsilon chain", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MQV7", "UniRef:UniRef90_A0A660MQV7" ], "nt": "ATGAACACGATCACTCACAAAAAAGATCAGATTTTTTGCTTTTTCATTACTGAACGACTATACTTAGGCGAGTTTATCTTCTTAATGTTAAGAATTATGCAACTTACTATAGCTACGACACAAAATACGACTGTTTATGAGGGGACTATAGACCAGGTGAATTTGCCAACTGAAAATGGGACTTTAGCAGTTTTCCAAAATCATATTCCCTCGGTGGTGAAACTGGTTCCTGGAGTGATTCAGATTTCTTCTGGTGGGAAAATAAGTAAGAGTCTGAGCATTTCCAAAGGGATTGCTTTGGTGGATGGAAGTATGATTAAGATTACTGTTTCTGTGGCTACCATGCAACCTTTGGCGAAACTGGTGGAGCTTCGTGGAAATCAACAACTTCTTGAACTGAAATTACAGAAAGTGAAAAGCTTTGGAAGTGTGGAGGAGATTAGTCAGCTGATTTGTGAGTTAGAGAAAGTTAAAGCTGATATTAAACTCGCAGAACTTTATCATTAA", "aa": "MNTITHKKDQIFCFFITERLYLGEFIFLMLRIMQLTIATTQNTTVYEGTIDQVNLPTENGTLAVFQNHIPSVVKLVPGVIQISSGGKISKSLSISKGIALVDGSMIKITVSVATMQPLAKLVELRGNQQLLELKLQKVKSFGSVEEISQLICELEKVKADIKLAELYH", "aa_hexdigest": "c05bd0c46e99170449e03fd032f26fce", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A660MQV7", "query_cov": 0.8333333333333334, "subject_cov": 1.0, "identity": 0.986, "score": 254.0, "evalue": 7.21e-85, "valid": true, "product": "ATP synthase epsilon chain", "uniref50_id": "UniRef50_A0A660MQV7" }, "pscc": { "uniref50_id": "UniRef50_A0A660MQV7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MQV7" ], "product": "ATP synthase epsilon chain" }, "genes": [], "id": "NKOCPLAKPK_833", "locus": "GCA003260355_00797" }, { "type": "cds", "sequence": "contig_1", "start": 427726, "stop": 427971, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAAATGATAATCTGCGTTTAGATTATGGTGAAGGATTGAGTTTTGATCTTTCGTATTTGGAAATCTATTCTGGAGTAAAAGTAAAAATTACTGTTTTAACAGGAATGGTAGAAGTAAATACTGAAGCTCATTATCGCACAAAAACTAAATCTTCATGTACTAGCGATAGTATCGCACCATTAAATGATAAACTATATTCGGGAATCGTTTTTGATAACAGAAATGAGTATCCTCCAATTTGA", "aa": "MKNDNLRLDYGEGLSFDLSYLEIYSGVKVKITVLTGMVEVNTEAHYRTKTKSSCTSDSIAPLNDKLYSGIVFDNRNEYPPI", "aa_hexdigest": "9fe5bb609cac0e6c0b33ea6786b32729", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9140.211699999996, "isoelectric_point": 5.205678749084471 }, "id": "NKOCPLAKPK_834", "locus": "GCA003260355_00798" }, { "type": "cds", "sequence": "contig_1", "start": 427975, "stop": 428163, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATCCGCCACTTACAAATCAAAAAATTATTCAAGCAGGGGAGTCAGCTATAATATCTGAAGAAGATGTGGTGGTGCTTTCAAATCTAAATGATCCAGAAGCTCAATATTGGGGATTTGATATGAAAATTAGTCCACTAGCCAAAGAAAATCGTATTGCTTATGAAATCATCATTCCAAAAGATTAA", "aa": "MYPPLTNQKIIQAGESAIISEEDVVVLSNLNDPEAQYWGFDMKISPLAKENRIAYEIIIPKD", "aa_hexdigest": "f785754804350cd9a64096d9e26b090e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7020.940099999999, "isoelectric_point": 4.398394584655762 }, "id": "NKOCPLAKPK_835", "locus": "GCA003260355_00799" }, { "type": "cds", "sequence": "contig_1", "start": 428345, "stop": 428593, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCTTTTTGAGAATTGTGAGGAGGCTAAGCTTGCCCAACTCGCTGGGATTATCAAGTGGGGAAAGCTGATCGATGGTGATTTGTCAGACTTTTTTTCTTCTTGTGAAAAGAGAATCCTCGGAGTAGCAGATAAAAATCAGGGAATCAATCTCAAAAAACGTTATGGACTCAAGAGATTTAAGCAGGTCGATCTTTTTCATACGGATTTGGAGGTTAAGCAAAAGGGGGTCGAACTCATAAAAATCTGA", "aa": "MLFENCEEAKLAQLAGIIKWGKLIDGDLSDFFSSCEKRILGVADKNQGINLKKRYGLKRFKQVDLFHTDLEVKQKGVELIKI", "aa_hexdigest": "a26fc59696718c3be573ef14b0ab2db8", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9383.913499999997, "isoelectric_point": 8.951000022888184 }, "id": "NKOCPLAKPK_836", "locus": "GCA003260355_00800" }, { "type": "cds", "sequence": "contig_1", "start": 428609, "stop": 429004, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTGGGTTATCAGCAGATTAAACTTTATGAGGTGGTGGATTTTGAAAAGCCAGGTCGTAGTATGCAAATGGGGATGATGCCTGCGAAATTAACGCATACTTTGATGAATATCGGGCTTTCTCTTTCAAAATCACAGGAGAAACCGCTGATTTATGATCCTTTTGCTGGAAGTGGAACGACTGGTTTTTTGGCAAATTATTTTGCTTATGATTTCTTAGGTTCAGATTTGAAACTTACCTTTGCAAATCAGAATAAACCTTGGTGGCTCGCTTCTAAATGGTCAAAACCTGATCAGATTTTTGATTTTTTCTTACATAACGCTACAAAAAGCTTTGAAAATCCTGAGCTTTTTGCTGGAAAGACTCCGATTATTATTACTGAAGGGTGGCTTTGA", "aa": "MLGYQQIKLYEVVDFEKPGRSMQMGMMPAKLTHTLMNIGLSLSKSQEKPLIYDPFAGSGTTGFLANYFAYDFLGSDLKLTFANQNKPWWLASKWSKPDQIFDFFLHNATKSFENPELFAGKTPIIITEGWL", "aa_hexdigest": "99c4465fdeac0427ea91c308a1cd6158", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14940.081399999983, "isoelectric_point": 6.720665168762207 }, "id": "NKOCPLAKPK_837", "locus": "GCA003260355_00801" }, { "type": "cds", "sequence": "contig_1", "start": 429059, "stop": 429289, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAAAAATGTTTATCGTCCTTGGATTGAACAAATGGCGAATGCTTTCCCTAAGAAACCGACGATGGTTTTTACTATTCCTCGGTATTTGGGCTATGAGAATTTGCTAGAAAAGGCAATTATTGAAGTAGCTGAGCAGGTGGGCTTTCGTTTTGGTTCGCTTCAAGAGCTTTACAAAAGAGAGCAACATAAAGTAGCAAGAAAAGTTATCTTTCTGACGGGGGAGAAGTAA", "aa": "MKNVYRPWIEQMANAFPKKPTMVFTIPRYLGYENLLEKAIIEVAEQVGFRFGSLQELYKREQHKVARKVIFLTGEK", "aa_hexdigest": "8f0003910c689a5c8f09ea38da8ffa5b", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9019.521899999996, "isoelectric_point": 9.69528751373291 }, "id": "NKOCPLAKPK_838", "locus": "GCA003260355_00802" }, { "type": "cds", "sequence": "contig_1", "start": 429560, "stop": 431152, "strand": "+", "frame": 2, "gene": null, "product": "DUF4384 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EJX7", "UniRef:UniRef90_A0A328EJX7" ], "nt": "ATGGGTTTTAAAACAATTCGTGAGTTAACGCTAACTTTTGGTCTTACTCACAAAAAAGGTATTCAACCTTTTTCCGCTATGGAGTGCTTTTATGCACTTAAGGCGATGTTTGATCGCAAACATCTGATGTTCGGTTCGTTTTTTCTTCCTCTTCTCCTTCTATTGCCTCTCAAGGCGAAGGGTCAGAATTCAGTTTCTGGCACTACTGGAAGGGCTCGGGAGGAAATAACGACAAGCTCTGCCGCTGCTTCTCAGCAGAAGGCGGATGCACCTTCCTCCACTGTTTCAGACAAGACTTCTGTTCGTTCTAAGTGCTTAAGCCCTGAGGAATGTATGAAGATTGTTCAGGAGGACGCCTATAAAAGGAATCCCGAGCTTCGGGAATATGATCTTGCTCAAGCAGAGAAGAAAAAATCAGAAGCTCAACAAGGGATGACTCAGAAGAAAGCTTCTCGGTCTGCTGTTCAGAAAGTTTCTGCTCCGGTTGCAGGCAAAAAGTCTGTAGCTCGGAAGAAGCCTACCCCAGCGGAAAAGAGAAAGTCTCAAGCTCAACAACGGCCAAGCCACAAGAAAGCTCCTCAGCCTTCTCTTCGGAAGGGGGCTGATCCAGGCAGAGAAAAAATGACTCTTGCTCGGCAGCATTCTCTTCCGGCACAGGCGAAGAAGTCTGATGCTGGACAAGGAAGGTCTTCGGGAAAAGATTCGAAGCTTCTTGCTCAACGTCAATCAAGCTCTTCAAAGAGGACTAAATCTACAACCCGCAAGTCTCCCTCTCGTCCAAAAGCAAAAAATCTTCAGGTGCGGGAAGGGATTCTTCAATCAAGGGTGATTCAGGCTTTGGATCCTCCTGCCTTTCCTCCTATTGATCCGTCGTTGCCGAAGGTCTTTGCGAGGATTGAATCCTCTTCACGAGGTGCTAGAGTGTCGGCTGATGTGCATGAATCACTTGTTCAGGCTGGTCAGATAGAGCTTGTTGTCTGTGATGGTCAATCTGGGGTTTTGGTGTTCTATGGTGAAATGGTTGAAGGGGTCACTTCTCCTGATGGTTATGTCCGCTTTGAGGTTACTTGTCCTGCTTCGTGCTTCAAAAACGAGGAAGTCTACCTCTTTAGAGTGAATCAGTGGGGTCCTTTTGGAGGGCGTCAACTGACGGGCTTCCCGAGTTCGGTCTATTTCAAGGAGAATGATCCAGATGCTTCTGTTCATGTGGAGAAAACACCGGTTAAGCATCAAACCACCCTGCGCATTGTTCCGGTTGGTGATGGTGCGAAGTTCGTTGTCAGGACTACGGAGAAACCTAGGCGTGATGAACGTATCTACATCCAGGTTTATGATCCTTTTGATAACTTTTATGCCATTCTCCCTATGCAACTTGACTCAAAGCATGGGGATTTGGCGGTCTATACCTTCTTGGAGAAGGATATGTCCCAGTATCTTTCGGTGCCGAGGGAACGGAGATTCCGTGTATATCTCGGGGAGCTGCCTCTTTTCGAAGAGACTCCGACCGGTAGACAACTGTCTGGTTGTAAAACAGATTTAAGTCATTTTCCCTTTTATCATAATTATGGAGAGCTTGTTCTTTCCGCGTGGTAG", "aa": "MGFKTIRELTLTFGLTHKKGIQPFSAMECFYALKAMFDRKHLMFGSFFLPLLLLLPLKAKGQNSVSGTTGRAREEITTSSAAASQQKADAPSSTVSDKTSVRSKCLSPEECMKIVQEDAYKRNPELREYDLAQAEKKKSEAQQGMTQKKASRSAVQKVSAPVAGKKSVARKKPTPAEKRKSQAQQRPSHKKAPQPSLRKGADPGREKMTLARQHSLPAQAKKSDAGQGRSSGKDSKLLAQRQSSSSKRTKSTTRKSPSRPKAKNLQVREGILQSRVIQALDPPAFPPIDPSLPKVFARIESSSRGARVSADVHESLVQAGQIELVVCDGQSGVLVFYGEMVEGVTSPDGYVRFEVTCPASCFKNEEVYLFRVNQWGPFGGRQLTGFPSSVYFKENDPDASVHVEKTPVKHQTTLRIVPVGDGAKFVVRTTEKPRRDERIYIQVYDPFDNFYAILPMQLDSKHGDLAVYTFLEKDMSQYLSVPRERRFRVYLGELPLFEETPTGRQLSGCKTDLSHFPFYHNYGELVLSAW", "aa_hexdigest": "e8ed932101502d70ac215cc5811c6195", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A328EJX7", "query_cov": 0.9509433962264151, "subject_cov": 1.0, "identity": 1.0, "score": 852.0, "evalue": 1.91e-309, "valid": true, "product": "DUF4384 domain-containing protein", "uniref50_id": "UniRef50_A0A328EJX7" }, "pscc": { "uniref50_id": "UniRef50_A0A328EJX7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EJX7" ], "product": "DUF4384 domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_839", "locus": "GCA003260355_00803" }, { "type": "cds", "sequence": "contig_1", "start": 431156, "stop": 431269, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EGL4", "UniRef:UniRef90_A0A328EGL4" ], "nt": "GTGTTGATGACTTCTTTAGAAGCTCCCTTGGGTTTTCCCGGGGAGCTTTTTCTTTTTGCTGGGAAAGGGGTGGATTACTGTTTGCTCGAGGAGGAAGAGAAGAAGGAAGTCTGA", "aa": "MLMTSLEAPLGFPGELFLFAGKGVDYCLLEEEEKKEV", "aa_hexdigest": "3058303be83de3a55e918b767ea75dd7", "start_type": "GTG", "rbs_motif": "TTCTT", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 431162, "stop": 431344, "observations": { "mutation": [ 431267 ], "stop": [ 431267 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EGL4", "query_cov": 0.9459459459459459, "subject_cov": 0.5833333333333334, "identity": 1.0, "score": 74.3, "evalue": 6.85e-17, "gene_start": 3, "gene_end": 37, "reference_start": 1, "reference_end": 35, "reference_sequence": "MTSLEAPLGFPGELFLFAGKGVDYCLLEEEEKKEVGFFFCFSLAIEERILIYLLASHSVP" }, "paralog": false, "identity": 0.9833333333333333, "score": 120.0, "evalue": 1.58e-37, "description": "Internal stop codon at 431267. Nonsense mutation around 431267.", "psc": { "uniref90_id": "UniRef90_A0A328EGL4", "uniref50_id": "UniRef50_A0A328EGL4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EGL4", "UniRef:UniRef50_A0A328EGL4" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EGL4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EGL4" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 4134.784900000001, "isoelectric_point": 4.216452980041503 }, "id": "NKOCPLAKPK_840", "locus": "GCA003260355_00804" }, { "type": "cds", "sequence": "contig_1", "start": 431359, "stop": 431553, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGAGGTCGCGTATTTTTGGTTGTAGCTCCCCGGGTTTTTTACCTGGGGGGCTTTTCCGTTTCGGGGAAAAAAGTCAGATTTTTCTGTTTTTCTCTTGCAAATGAGGAGGATATCCGTATCTTAGTGGCACTTCTGTTAAGTGGAATTAATGTCTTTAAGTGCGCTGGTCGTGAGACTCGCAATGCTTCTTAG", "aa": "MRGRVFLVVAPRVFYLGGFSVSGKKVRFFCFSLANEEDIRILVALLLSGINVFKCAGRETRNAS", "aa_hexdigest": "a0c0546566dde32631d12afe2753f01b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7127.367799999999, "isoelectric_point": 10.418364906311034 }, "id": "NKOCPLAKPK_841", "locus": "GCA003260355_00805" }, { "type": "cds", "sequence": "contig_1", "start": 431990, "stop": 432505, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTTACTTTCTCCAGTCCCCCCTCTCCCCGTCATTGCGAGCGAAGCGAAGCAATCCAGTCCTTCCTCTCCCGTCATTGCGAGCGAAGCGTGGCAATCCAGTACCCCGGGCATCGAATTTGGGGGGTCGTTTTACCCTTCCGATCACCCCGGGCATCAAATTTGGGGGTCATTTTCACCTTCCCGACAGCTCGGAAGTCAAATTTGGGGGTCATTCTTGCATCATCGACCTGTCCGACACCCTAATTTGGGGGTCAAGCTTCCCGTCCCCCATAAACTTCACACCCCGCTCCTCCCAAAACCAACGAGCCAAAGCGAATCCCCTCATCTATTTTTTGCCGCCCCAAGAGGCGACCACAGGGAGTCCTCGACGAAACGAAGTGGAGGTCAAGAGGAGCTGGCACGAAGTGCCTGAGGGGTTGAGTGGAAGTCGGGAGGGCAATTCTGAAGGCAGCGGGAATTTAGTCCCATTACCACGATTGCTCTTCACCTTTAAACTTCGTTCTCTACTCTAG", "aa": "MFYFLQSPLSPSLRAKRSNPVLPLPSLRAKRGNPVPRASNLGGRFTLPITPGIKFGGHFHLPDSSEVKFGGHSCIIDLSDTLIWGSSFPSPINFTPRSSQNQRAKANPLIYFLPPQEATTGSPRRNEVEVKRSWHEVPEGLSGSREGNSEGSGNLVPLPRLLFTFKLRSLL", "aa_hexdigest": "887fd984ad5f5b55eeed142b1cc2a6b3", "start_type": "ATG", "rbs_motif": "TTTTT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18830.308299999993, "isoelectric_point": 10.665665626525882 }, "id": "NKOCPLAKPK_842", "locus": "GCA003260355_00806" }, { "type": "cds", "sequence": "contig_1", "start": 432575, "stop": 433030, "strand": "+", "frame": 2, "gene": null, "product": "Yip1 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EKJ9", "UniRef:UniRef90_A0A328EKJ9" ], "nt": "ATGAATCTAAAAATGAAGAAAAGTAAATGGCTTCCAGCAGGTTTTTGGGCTGATTTTGATACGACGTTTTCACGTCTTTTTACAGGAGCGAACTTGGTTGCCCTCGGGTTTTCTGGGCTTTACTGTGCGATCTCTTGGATCTTGGAGAGGATATATTCGTCTCTTTTTTCTGGATTGCTAGGGGTTTATTCTATTATCGGGAATGGAAGTCTCAAAGGTGAGCTTTTGCAGCGTTGGCTACCCGCAGGAGATGCTTTTTTCCCCAATATGTGGTATCTTTCAGTGATTGGGCTTGCTGTCGTGTCCTTGAGTTTCGTCATTGCACAGCTTGTGAATAAGAGAAAGGGGAATTTGACCAGATTTTCGTTCTGGTTGATGCTGTTTTTCTTTGTGCTGTTGGCATTACCGTATGTTACGATGTTTTGCAACGGGATCGGTTACTTTTTGCAGCAGTAG", "aa": "MNLKMKKSKWLPAGFWADFDTTFSRLFTGANLVALGFSGLYCAISWILERIYSSLFSGLLGVYSIIGNGSLKGELLQRWLPAGDAFFPNMWYLSVIGLAVVSLSFVIAQLVNKRKGNLTRFSFWLMLFFFVLLALPYVTMFCNGIGYFLQQ", "aa_hexdigest": "d5d828d3c1629e46e6eeb506f4a8b9a2", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A328EKJ9", "query_cov": 0.9735099337748344, "subject_cov": 1.0, "identity": 1.0, "score": 301.0, "evalue": 1.63e-103, "valid": true, "product": "Yip1 domain-containing protein", "uniref50_id": "UniRef50_A0A328EKJ9" }, "pscc": { "uniref50_id": "UniRef50_A0A328EKJ9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EKJ9" ], "product": "Yip1 domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_843", "locus": "GCA003260355_00807" }, { "type": "cds", "sequence": "contig_1", "start": 433142, "stop": 433384, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGCCAAAACCAAAAACCTCGGAATGCTCGTGTTGATTTCATTAGTCATCATCGGACTCTATATAGAATCTTGTCAAAGAGTCCGGCGTAATCCCTCCCTGGATCAAAATAACCTCCAGTTCCCGCCGGCTACAGAAGATACGCTCACGTGGAGAGATTCTCTTGAAGACGTGCAAGCACAAGTGGCAAACGAAAAAGGCACCCACTGCCTCATCAACCTCTACGAGGACCAAAACTGA", "aa": "MKAKTKNLGMLVLISLVIIGLYIESCQRVRRNPSLDQNNLQFPPATEDTLTWRDSLEDVQAQVANEKGTHCLINLYEDQN", "aa_hexdigest": "7e35fdfa44c59c831b26317403c20d3e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9116.311299999998, "isoelectric_point": 5.18027172088623 }, "id": "NKOCPLAKPK_844", "locus": "GCA003260355_00808" }, { "type": "cds", "sequence": "contig_1", "start": 433604, "stop": 433894, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DC04040", "UniRef:UniRef100_A0A328EGS0", "UniRef:UniRef50_A0A328EGS0", "UniRef:UniRef90_A0A328EGS0" ], "nt": "ATGAACAAAAAGAAAGAGACAAAAGGTGAATTTGTAGGAATGCTGGCAATAGCTTGTGTAATAGCCGCTATGCTCATGCCCCCAAAAGTAAAATCTGGCTCCAGCCTTGAAGGCGAGCGAAAAGTCGACGAATGGGAGATCCCGATCAAGGAAAAAGACCCCAAGCTTGCCTTGGAGAAAGATCAAGACTTCTTTAGTCCGGACAGCTTGATGAAAGAGGTCGTAGAAAGAATGAGAGAAAAACGTCCTCTTTTTGACAGACAGATCTTCGAATTTCGAGCAGAATGGTAA", "aa": "MNKKKETKGEFVGMLAIACVIAAMLMPPKVKSGSSLEGERKVDEWEIPIKEKDPKLALEKDQDFFSPDSLMKEVVERMREKRPLFDRQIFEFRAEW", "aa_hexdigest": "351864714d3f67f71c99cc3566c3ab14", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000DC04040", "uniref100_id": "UniRef100_A0A328EGS0", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DC04040", "UniRef:UniRef100_A0A328EGS0" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EGS0", "uniref90_id": "UniRef90_A0A328EGS0" }, "psc": { "uniref90_id": "UniRef90_A0A328EGS0", "uniref50_id": "UniRef50_A0A328EGS0" }, "pscc": { "uniref50_id": "UniRef50_A0A328EGS0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EGS0" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11233.049699999992, "isoelectric_point": 6.3563840866088865 }, "id": "NKOCPLAKPK_845", "locus": "GCA003260355_00809" }, { "type": "cds", "sequence": "contig_1", "start": 434673, "stop": 434915, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCTATAATTCAAGAAGAGTGTGAAGATTCAACTTATGATCAAAGAATTTATCAGGAAAGTTTGAGCTTTTTATTAAGTCAAAAGTTTGATTTGAACTCTGCTTCTGGACTTTCTTGGGAAGAGGTACAAAAATATCATGAAAAATATTATAAACTTGAAAATTTTCTTGTTTGTGATGATTCACTTCCTGATTATAGACTTCTTTTTCAAGGAGAAAAATTGAAAAATTTAAGAAGTTGA", "aa": "MPIIQEECEDSTYDQRIYQESLSFLLSQKFDLNSASGLSWEEVQKYHEKYYKLENFLVCDDSLPDYRLLFQGEKLKNLRS", "aa_hexdigest": "7150b7d9248ef10d731560d9bff34060", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9607.641499999992, "isoelectric_point": 4.611427116394043 }, "id": "NKOCPLAKPK_846", "locus": "GCA003260355_00810" }, { "type": "cds", "sequence": "contig_1", "start": 434959, "stop": 435129, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTGTATTTTATTTATTGAAAGCAATATTGTCTTCAGGAGGATTATTGGAAACTCTTTTTTCTTCAAACACTGTTGGAGTGATATATTTTTTTTCAGAAAAGATGGAAATGAAGAGAATATTATCATGGGACTCCTTATTTTTTTACTTATCAAGAATCAAATTGGTTAG", "aa": "MCILFIESNIVFRRIIGNSFFFKHCWSDIFFFRKDGNEENIIMGLLIFLLIKNQIG", "aa_hexdigest": "76299cc447a0529df1673aa1826c8351", "start_type": "ATG", "rbs_motif": "TTTTT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6702.929199999997, "isoelectric_point": 7.825768852233888 }, "id": "NKOCPLAKPK_847", "locus": "GCA003260355_00811" }, { "type": "cds", "sequence": "contig_1", "start": 435420, "stop": 435512, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAGTTTTGTTTGTCGTTGTGAATGTTGGGTTTGAATGTGATGAGTTTTCGATTCCTTATGAGGCAGTTGAAAAATGCGAGTTATGA", "aa": "MKKVLFVVVNVGFECDEFSIPYEAVEKCEL", "aa_hexdigest": "1dec2834a4962d802ff9045064971991", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3466.049100000001, "isoelectric_point": 4.420391273498535 }, "id": "NKOCPLAKPK_848", "locus": "GCA003260355_00812" }, { "type": "cds", "sequence": "contig_1", "start": 435969, "stop": 436391, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATCGCTCTTTGTTCTTCGATCTGCCTGCGCATTTCGTATTGCATCGTCAAGACGCCGCTTATGACCATCCAGATGCCACTTATGACCATCCAGATGCCACTGATCGTAGGAAGCCAGGAATTGTTTTTGAAGATTGCTTTCATGATGCTGAAGTTTTTTTACTTGTCTTTAATTTCAGATTTCCAGAGTTTACTCATCATGATTGAGAGTATCCCTGCGGAGGATATACATACTAGGAATGCAGCTAAACTGAACTGCATATCTCTATTCGCAAAGAACTCAGGAATCCTACTCTGCAATTCGAAATTTTCGGTCAGCAGAGCAATGAAGTTTATTCCAGAGAAGACTGCCAATATTGCTAATATTCCTGCGCATAGATCCAAGAATACTATTAGCATAAATATCCACCTTCGTCGATAA", "aa": "MIALCSSICLRISYCIVKTPLMTIQMPLMTIQMPLIVGSQELFLKIAFMMLKFFYLSLISDFQSLLIMIESIPAEDIHTRNAAKLNCISLFAKNSGILLCNSKFSVSRAMKFIPEKTANIANIPAHRSKNTISINIHLRR", "aa_hexdigest": "554c4938cac6d7fa9ea1697534c04917", "start_type": "ATG", "rbs_motif": "TTCTT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15810.019299999985, "isoelectric_point": 9.718044853210447 }, "id": "NKOCPLAKPK_849", "locus": "GCA003260355_00813" }, { "type": "cds", "sequence": "contig_1", "start": 436644, "stop": 437435, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02492.25", "PFAM:PF00437.27" ], "nt": "ATGCCAGGAATCATTACCGAAAGCGTCGTGATCAGATTCCTTGATGCTACTGAAAGTATCGATGATTTTAGAGAAATCGGCTTCAGGGGAGATAACCTTAGCGTCCTAGAAAACTACCTTCACAAAACCAGCGGAATCATCATCATCACCGGACCAACCGGATCAGGAAAGACCACAACACTCTATGCCATTCTCAAAAAACTCAACGACGGAACCAAAAAAATCATCACACTTGAGGATCCTATCGAATACAAAATCGCAGGACTCCAACAATCTCAGATCAACTACGACAAAAACTACGACTACGAAACCGGACTAAAAGCCATCCTTAGACAAGACCCGGACATCATCCTCGTTGGAGAAACCAGAACCAAAGAAACCGCTCAAATCGCCATCAATGCTTCTCTTACAGGACACTTAGTTTTTACAACTTTGCATACCAATTCAGTGCTCGACTCTCTCTCAAGACTCATGAATATGGGAATCGAACCCTACCTCCTCACTCCAGCCCTCCAATTACTCATTGGACAGAGGCTCGTTCGTAAAGTCTGCCCATTTTGTAGCGAATGGCAGGATGCGACACCAGAAGAAGACGCAGAAATAAGAAAAAACCTGACTGAACTCCAGGAACGCCTCCCAACAGACGTATTACCTGACTATCAAGGAAAAATCCTCAAAGGGAAAGGATGTGAACATTGCAATCACTCAGGCTACAAAGGCAGAATTGCTCTCATAGAACTCCTCGAAATCAACGACATCATGAGAGAAAAACTCATGCAAGGAATGCAATGA", "aa": "MPGIITESVVIRFLDATESIDDFREIGFRGDNLSVLENYLHKTSGIIIITGPTGSGKTTTLYAILKKLNDGTKKIITLEDPIEYKIAGLQQSQINYDKNYDYETGLKAILRQDPDIILVGETRTKETAQIAINASLTGHLVFTTLHTNSVLDSLSRLMNMGIEPYLLTPALQLLIGQRLVRKVCPFCSEWQDATPEEDAEIRKNLTELQERLPTDVLPDYQGKILKGKGCEHCNHSGYKGRIALIELLEINDIMREKLMQGMQ", "aa_hexdigest": "460772ec4550533acba38f4ca48c7625", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_K2EX78", "query_cov": 0.8593155893536122, "subject_cov": 0.8560606060606061, "identity": 0.5, "score": 214.0, "evalue": 1.28e-65, "valid": false, "product": "AAA+ ATPase domain-containing protein", "uniref50_id": "UniRef50_K2EX78" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02492.25", "name": "cobW", "length": 35, "aa_cov": 0.13307984790874525, "hmm_cov": 1.0, "evalue": 1.6441576368217578e-05, "score": 20.975770950317383, "start": 46, "stop": 80 }, { "id": "PF00437.27", "name": "T2SSE", "length": 260, "aa_cov": 0.9885931558935361, "hmm_cov": 0.9, "evalue": 1.196198473892273e-84, "score": 280.52642822265625, "start": 1, "stop": 260 } ], "seq_stats": { "molecular_weight": 29521.652900000037, "isoelectric_point": 5.355278968811036 }, "id": "NKOCPLAKPK_850", "locus": "GCA003260355_00814" }, { "type": "cds", "sequence": "contig_1", "start": 437676, "stop": 438116, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTGGACTCATAAAAACTATTCTTGAACAGGAAGAAATCCAAGGGCTCAATCCAGACTTCTCTCTTTTGACCATAATTCCCAAAGCTACCGCAGAAAAGGCGCAGCTCCTTATTTTCGGGAAAGGGGAAAAAAGAACACTCAAGCTTCTTACGACCAATAACCACCCAGAGGCAGTCAAACAGCTTCTGAATCAGATCGAACAAAAGGGCTATCACCATGAACTCTTCTATACCACAAACGAAGGCTTCTCAACGGCGCTCGGACGATATACAATTTTAGAAGAACAAGAAGCTCATAGAGCAGAACTCATCAAACAAGAAGCAAAAGCTGAAGGGAAATCAGCAGTCGCCTTGATGCAAGAACAATTTGAACAGAGAGAAAGCCAAGATCCTACTCAATTCATCAATTCAATCATCAGACTGAGCTTCCAAGCATGA", "aa": "MAGLIKTILEQEEIQGLNPDFSLLTIIPKATAEKAQLLIFGKGEKRTLKLLTTNNHPEAVKQLLNQIEQKGYHHELFYTTNEGFSTALGRYTILEEQEAHRAELIKQEAKAEGKSAVALMQEQFEQRESQDPTQFINSIIRLSFQA", "aa_hexdigest": "e90727bd6c1d315d26294bc2df765037", "start_type": "ATG", "rbs_motif": "TTTA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16568.656299999995, "isoelectric_point": 5.608098793029785 }, "id": "NKOCPLAKPK_851", "locus": "GCA003260355_00815" }, { "type": "cds", "sequence": "contig_1", "start": 438202, "stop": 438432, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTTGCTGTTCGATTTTGATGAGTGCGGTCGAGAAGGGGAGCTCTAAGAGTTTTGTTTCAAAGTATTTAAAGCCTCCGCTGATGATCATCCCTCCAGTAATGATGAGAAGAATTCCGATACTCTTTTTGAATCGTCAGTCGGGGTTTGCGTATCCTTTTAGTCTTTTTATAGCTTCTCTTCAAAAGAAGATAATGATAAAAAGAATACTTCAGAATCCTAAAACATAG", "aa": "MVCCSILMSAVEKGSSKSFVSKYLKPPLMIIPPVMMRRIPILFLNRQSGFAYPFSLFIASLQKKIMIKRILQNPKT", "aa_hexdigest": "394ac0b01193824753c16daa46a739b2", "start_type": "ATG", "rbs_motif": "TTATT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8677.665999999994, "isoelectric_point": 10.577086067199705 }, "id": "NKOCPLAKPK_852", "locus": "GCA003260355_00816" }, { "type": "cds", "sequence": "contig_1", "start": 438905, "stop": 439108, "strand": "-", "frame": 3, "gene": null, "product": "Thioredoxin domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EGU1", "UniRef:UniRef90_A0A328EGU1" ], "nt": "GTGCTCCAAGATGAATTAACGACAAAAGGAATCCCAGCTGGCAGCATCTTATTCTTGATTGATTTCGATAACGCAAAAGAGTTAAGAGCAACCTACAATATAAACAATATGGATACCTTCATAACAGTAAATGAAAACTTACAAGAAACCCATAGAGAAAACCCTGGGAACTATGAAACAATTTATCAACTACTACAAAACTAA", "aa": "MLQDELTTKGIPAGSILFLIDFDNAKELRATYNINNMDTFITVNENLQETHRENPGNYETIYQLLQN", "aa_hexdigest": "7dd227ca613f8a6f5bf5dea5ab97b0e4", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 438905, "stop": 439336, "observations": { "mutation": [ 439147, 439210, 439219, 439285, 439291, 439309 ], "stop": [ 439147, 439210, 439219, 439285, 439291, 439309 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EGU1", "query_cov": 1.0, "subject_cov": 0.46853146853146854, "identity": 0.94, "score": 132.0, "evalue": 3.6e-38, "gene_start": 1, "gene_end": 67, "reference_start": 77, "reference_end": 143, "reference_sequence": "MTKKLVFGLGFFLIFGVGTFIFLGNQGNCTAASCTETTVGTTGTILDYSPEEMANALSKKHKIGLLFKANRCGSCKVLQEELTTKGIPAGSILFLVDFDNAKDLRATYNINNMDTFITVNENLQETHRENPGNYETIYQLLQN" }, "paralog": false, "identity": 0.9370629370629371, "score": 266.0, "evalue": 1.54e-93, "description": "Internal stop codon at 439147, 439210, 439219, 439285, 439291, 439309. Nonsense mutation around 439147, 439210, 439219, 439285, 439291, 439309.", "psc": { "uniref90_id": "UniRef90_A0A328EGU1", "product": "Thioredoxin domain-containing protein", "uniref50_id": "UniRef50_A0A328EGU1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EGU1", "UniRef:UniRef50_A0A328EGU1" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EGU1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EGU1" ], "product": "Thioredoxin domain-containing protein" } }, "id": "NKOCPLAKPK_853", "locus": "GCA003260355_00817" }, { "type": "cds", "sequence": "contig_1", "start": 439869, "stop": 440174, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAACAATCCTTTCAACACTGTTGTTAATTACCTGTCAAAATTACAGGGAAAAATCATCACTAATGAAGTCATAAAAACAAAGCTTCAAAATATCCTAGACGAAGAGTATAGTGAACCTAAAATGTATAAAATTATCTATTATCTCAAACTCAGAGGACATCTCATCAATCTCAAAAAAAATCTTTTCCTTGTCAAAAAACCTGAAGATACAATAGATGAAGAAAAACTCATAAACAAAGTCTATCGAAGCATTCTCAATAAGCACTGCAATGATTTCATTGATGGAAAGCGATATATCTGA", "aa": "MKNNPFNTVVNYLSKLQGKIITNEVIKTKLQNILDEEYSEPKMYKIIYYLKLRGHLINLKKNLFLVKKPEDTIDEEKLINKVYRSILNKHCNDFIDGKRYI", "aa_hexdigest": "deb3aa0f729061ef2bbcd0750f81673a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12091.082899999981, "isoelectric_point": 9.470744132995605 }, "id": "NKOCPLAKPK_854", "locus": "GCA003260355_00818" }, { "type": "cds", "sequence": "contig_1", "start": 440349, "stop": 440627, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI000F13FF71", "UniRef:UniRef100_A0A328EJY3", "UniRef:UniRef50_A0A328EJY3", "UniRef:UniRef90_A0A328EJY3" ], "nt": "ATGCAAGGTTTTGAAATGCCACTTCTTACAACTTTAGACTTTAATCAAAAAACTGTTTCTGTTGAGGGAATAAAGGCTGTGGTAACTCAAAATTCATCTTCAGATTTTGAAGTTTTGCTTGAAATTAATTCAAATAAGATCCTGATTGCTTTGCAGGGGGCTTATGATGCTATAGAACAGTTTGAAATCTTGGCAGTGAAGGGGTTTGTAGAGTTGAGTGTTTCTTTTATTAATACTATAAAGAAATTAGTAGGACATCTTTTGTGTAGATTAGATTAA", "aa": "MQGFEMPLLTTLDFNQKTVSVEGIKAVVTQNSSSDFEVLLEINSNKILIALQGAYDAIEQFEILAVKGFVELSVSFINTIKKLVGHLLCRLD", "aa_hexdigest": "6f057f8887150874c5bfcd87d5481e4e", "start_type": "ATG", "rbs_motif": "TTTA", "ups": { "uniparc_id": "UPI000F13FF71", "uniref100_id": "UniRef100_A0A328EJY3", "db_xrefs": [ "SO:0001217", "UniParc:UPI000F13FF71", "UniRef:UniRef100_A0A328EJY3" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EJY3", "uniref90_id": "UniRef90_A0A328EJY3" }, "psc": { "uniref90_id": "UniRef90_A0A328EJY3", "uniref50_id": "UniRef50_A0A328EJY3" }, "pscc": { "uniref50_id": "UniRef50_A0A328EJY3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EJY3" ] }, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 440214, "stop": 440627, "observations": { "mutation": [ 440343 ], "stop": [ 440343 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EJY3", "query_cov": 1.0, "subject_cov": 0.6715328467153284, "identity": 1.0, "score": 176.0, "evalue": 3.7e-55, "gene_start": 1, "gene_end": 92, "reference_start": 46, "reference_end": 137, "reference_sequence": "MIFLKKSNRFFYKNSFVFSLVFLVENHTTMIVIVDVKFYFMPLGSMQGFEMPLLTTLDFNQKTVSVEGIKAVVTQNSSSDFEVLLEINSNKILIALQGAYDAIEQFEILAVKGFVELSVSFINTIKKLVGHLLCRLD" }, "paralog": false, "identity": 0.9854014598540146, "score": 261.0, "evalue": 4.13e-91, "description": "Internal stop codon at 440343. Nonsense mutation around 440343.", "psc": { "uniref90_id": "UniRef90_A0A328EJY3", "uniref50_id": "UniRef50_A0A328EJY3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EJY3", "UniRef:UniRef50_A0A328EJY3" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EJY3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EJY3" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 10188.74199999999, "isoelectric_point": 4.810761451721191 }, "id": "NKOCPLAKPK_855", "locus": "GCA003260355_00819" }, { "type": "cds", "sequence": "contig_1", "start": 440712, "stop": 440948, "strand": "+", "frame": 3, "gene": null, "product": "Iron-sulfur cluster assembly scaffold protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A948FN67" ], "nt": "TTGGTATTTATGTCAGAAACTGAGCTTTTAGTGAAGGAATATTCTAAAAATCCTTTGTGTAATTATGAAATGCAAAATCCTGATGTGACTAGACATGAAGGGAATTTTATTTGTGGTGATGATGTTACGGTTTATCTTAAGATTCAGGAGGGGAAAATAGCCGAATTTAGCTATGATGGGAATCCTAGCACGGTAAGTCTTGCAGCGGCGAGCTTTCTTTCGGAGTTTCTTATTTGA", "aa": "MVFMSETELLVKEYSKNPLCNYEMQNPDVTRHEGNFICGDDVTVYLKIQEGKIAEFSYDGNPSTVSLAAASFLSEFLI", "aa_hexdigest": "ca597ea16617fef72256133e0f62ff86", "start_type": "TTG", "rbs_motif": "TTTTTA", "psc": { "uniref90_id": "UniRef90_A0A948FN67", "query_cov": 0.9487179487179487, "subject_cov": 0.8043478260869565, "identity": 0.595, "score": 101.0, "evalue": 1.93e-26, "valid": false, "product": "Iron-sulfur cluster assembly scaffold protein", "uniref50_id": "UniRef50_A0A948FN67" }, "pscc": { "uniref50_id": "UniRef50_A0A948FN67", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A948FN67" ], "product": "Iron-sulfur cluster assembly scaffold protein" }, "genes": [], "id": "NKOCPLAKPK_856", "locus": "GCA003260355_00820" }, { "type": "cds", "sequence": "contig_1", "start": 440964, "stop": 441122, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A351Y477" ], "nt": "GTGTTGAGCTGGAATTATGAAACTTTTGTAGAGAAGGGATTTGAAGTTTCTCCTAGGAGGAGGAGAGCGGCCGTTATTGCTTTGCTCGGTGTGAGGAATGCGATCCATGCCTATCGTAATGATGGGATAGAGGAAGATTTTGATGACCTCATAGATTAG", "aa": "MLSWNYETFVEKGFEVSPRRRRAAVIALLGVRNAIHAYRNDGIEEDFDDLID", "aa_hexdigest": "8319b1e05c10ef815a2b7cba8bf15359", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A351Y477", "query_cov": 1.0, "subject_cov": 0.8, "identity": 0.635, "score": 67.8, "evalue": 5.83e-14, "valid": false, "uniref50_id": "UniRef50_A0A351Y477" }, "pscc": { "uniref50_id": "UniRef50_A0A351Y477", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A351Y477" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6069.7315, "isoelectric_point": 4.9375123977661115 }, "id": "NKOCPLAKPK_857", "locus": "GCA003260355_00821" }, { "type": "cds", "sequence": "contig_1", "start": 441225, "stop": 441620, "strand": "+", "frame": 3, "gene": null, "product": "Phage holin family protein", "db_xrefs": [ "KEGG:K08972", "SO:0001217", "UniParc:UPI000DBFE323", "UniRef:UniRef100_A0A328EKK8", "UniRef:UniRef50_A0A328EKK8", "UniRef:UniRef90_A0A328EKK8" ], "nt": "ATGTGGAAAATCGTAAAAGATATCATCCTTTCTGTGCTTATCAATGCGGGGATCCTGTATTTTGTGAGCAGTAATAAATTTGGTCTGGTGATTGAGTCGAAAGCTGATATTTTTCAGGTTTTTTTGCTTTTGGGATTGGTATTCTGGATTGTAAACTTTGGAATTAAGAGGATATTGCATATTATTACCTTTCCGCTAAAGTTTTTGACACTCGGGATTTCTTCAATTGTTATTAATGTAGGAGTTTTCTATTTCTTTCAATGGTTTATTAATGAGAATTGTAGTCAGTTTGCGAGGGTTCAGCTCCCTAGTGGAGATCTTGGATGGGTAAGGGTTTTGATTCTCTCAGTTATTATCTCAACAGTGTATGCTTTACTTAGTAAAATACTAAAATAA", "aa": "MWKIVKDIILSVLINAGILYFVSSNKFGLVIESKADIFQVFLLLGLVFWIVNFGIKRILHIITFPLKFLTLGISSIVINVGVFYFFQWFINENCSQFARVQLPSGDLGWVRVLILSVIISTVYALLSKILK", "aa_hexdigest": "5e3d85f7ae2ca84065778b11ab7559e4", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000DBFE323", "uniref100_id": "UniRef100_A0A328EKK8", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBFE323", "UniRef:UniRef100_A0A328EKK8" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EKK8", "uniref90_id": "UniRef90_A0A328EKK8" }, "psc": { "uniref90_id": "UniRef90_A0A328EKK8", "product": "Phage holin family protein", "uniref50_id": "UniRef50_A0A328EKK8", "kegg_orthology_id": "K08972" }, "pscc": { "uniref50_id": "UniRef50_A0A328EKK8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EKK8" ], "product": "Phage holin family protein" }, "genes": [], "id": "NKOCPLAKPK_858", "locus": "GCA003260355_00822" }, { "type": "cds", "sequence": "contig_1", "start": 441620, "stop": 441790, "strand": "+", "frame": 2, "gene": null, "product": "Lipopolysaccharide assembly protein A domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EGT0", "UniRef:UniRef90_A0A328EGT0" ], "nt": "ATGAAAAACGGAATGCGATGGTCTTTGGGGGCGATGCTGCTCTTTTGTGTGCTTGCCTTTATTGGAGTGGTGCAGAATGCAGGGGCGTCAGCAACCTTTAGTTTTCTGGGGATGCGTTCTCTTTCCTTCTTTAAATTGGGAGGGCTCCTGATGATTTGTAGTATCTTGTGA", "aa": "MKNGMRWSLGAMLLFCVLAFIGVVQNAGASATFSFLGMRSLSFFKLGGLLMICSIL", "aa_hexdigest": "fd272d04989a080c4a6dba7c312fcc26", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 441620, "stop": 441880, "observations": { "mutation": [ 441788 ], "stop": [ 441788 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EGT0", "query_cov": 1.0, "subject_cov": 0.6511627906976745, "identity": 1.0, "score": 110.0, "evalue": 2.03e-30, "gene_start": 1, "gene_end": 56, "reference_start": 1, "reference_end": 56, "reference_sequence": "MKNGMRWSLGAMLLFCVLAFIGVVQNAGASATFSFLGMRSLSFFKLGGLLMICSILGGVFLVLFIQNLFSGIKRPELTKFDLDKPL" }, "paralog": false, "identity": 0.9883720930232558, "score": 168.0, "evalue": 8.17e-56, "description": "Internal stop codon at 441788. Nonsense mutation around 441788.", "psc": { "uniref90_id": "UniRef90_A0A328EGT0", "product": "Lipopolysaccharide assembly protein A domain-containing protein", "uniref50_id": "UniRef50_A0A328EGT0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EGT0", "UniRef:UniRef50_A0A328EGT0" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EGT0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EGT0" ], "product": "Lipopolysaccharide assembly protein A domain-containing protein" } }, "id": "NKOCPLAKPK_859", "locus": "GCA003260355_00823" }, { "type": "cds", "sequence": "contig_1", "start": 441905, "stop": 442510, "strand": "+", "frame": 2, "gene": null, "product": "Helix-turn-helix domain-containing protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBF59A4", "UniRef:UniRef100_A0A328EK19", "UniRef:UniRef50_A0A2G6CAL3", "UniRef:UniRef90_A0A328EK19" ], "nt": "ATGAAAAATTATTCAATTACGAGAGAAAAAGCTGCGATTGAGCTTGGAGTTTCTACGAGAACGATTGATAGATATGTGAAAGGTGGAAAACTTTCTTATAAAAAGGTGGCGAATAAGGTTTTGCTTGCGAGAGATGAGGTAGAAGCGTTAAAAGCTGAATTTTCAACCCTTCATAGTCAGGTAACTAGTGAACTTATCCAAGAAGTTAAGCCAAACGTTGCTCCAACGAGTCAGACTTCTCCTGTTGCTCAGCCAGTAGCCGTTAGAGCGGATATTGATCAGGCGATTGAGGAGAAAATCGATAAATTTTTCTTGATTTTCAAGGAGAAGGACAAGATGATTGAGGAAAAAAATAAGATTATCTTTATGCTTCAGCAGAGGATTGGGGAATTGGAGACCAAGATTCAGACGATGATCGCTCTCCCTGATTATAATAGAGAAAAACAAGAAGCTATTATTGAAAAGCAAAAGCTTGAGGAGAGAATCTCCAAACTCAAAGAAGCTGTAAGAACAGAAAAGGTGAAAAATTTTATTATTATGCTGTTTTCTTTGCTGTTTATCGGGATTGCGATTTTCTTGTTGATGAAATATAAGATTATTTCTTAG", "aa": "MKNYSITREKAAIELGVSTRTIDRYVKGGKLSYKKVANKVLLARDEVEALKAEFSTLHSQVTSELIQEVKPNVAPTSQTSPVAQPVAVRADIDQAIEEKIDKFFLIFKEKDKMIEEKNKIIFMLQQRIGELETKIQTMIALPDYNREKQEAIIEKQKLEERISKLKEAVRTEKVKNFIIMLFSLLFIGIAIFLLMKYKIIS", "aa_hexdigest": "2f007d032b1a077191c0c8d888bc4029", "start_type": "ATG", "rbs_motif": "TTTTT", "ups": { "uniparc_id": "UPI000DBF59A4", "uniref100_id": "UniRef100_A0A328EK19", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBF59A4", "UniRef:UniRef100_A0A328EK19" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EK19", "uniref90_id": "UniRef90_A0A328EK19" }, "psc": { "uniref90_id": "UniRef90_A0A328EK19", "product": "Helix-turn-helix domain-containing protein", "uniref50_id": "UniRef50_A0A2G6CAL3" }, "pscc": { "uniref50_id": "UniRef50_A0A2G6CAL3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2G6CAL3" ], "product": "Helix-turn-helix domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_860", "locus": "GCA003260355_00824" }, { "type": "cds", "sequence": "contig_1", "start": 443140, "stop": 443544, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF17191.10" ], "nt": "ATGGAACTCAAAACGCTCCTCAGGACCACCCCACGCTATCTTAACCTCTTGGCACAGAATGGTATCACGACCATTAGGGATTTTTTTAATTATTTTCCGAGAGCCTACGAAGACAGAGCGAATATCAAGCCTCTGAACCAACTGATTTTTAATGATAAAGGTATCACTGCAACTAAAGGAAAAATCCTGAAAAAAACCGTCTTTCGCAGAGGGGGGAGAATGATTTACGATATTAGTTTTGAAGATGAACTAGGGATTCAAGGGACGATTTCGATTTTTAATTCAGGCTATCTTGCCAGCAAGCTCCAAGAGGGATGCCGATACATTATTGTAGGGAAGCCGAGCTTTAAGTATGGGAAAACGATCTTTTCCCACCCTGATATTGTGCCGAGTTCGGATTCTTGA", "aa": "MELKTLLRTTPRYLNLLAQNGITTIRDFFNYFPRAYEDRANIKPLNQLIFNDKGITATKGKILKKTVFRRGGRMIYDISFEDELGIQGTISIFNSGYLASKLQEGCRYIIVGKPSFKYGKTIFSHPDIVPSSDS", "aa_hexdigest": "e8215f8e704b893a96aa4d0e93806333", "start_type": "ATG", "rbs_motif": "TTATT", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF17191.10", "name": "RecG_wedge", "length": 124, "aa_cov": 0.917910447761194, "hmm_cov": 0.9758064516129032, "evalue": 2.0169988175093483e-17, "score": 59.6925163269043, "start": 10, "stop": 132 } ], "seq_stats": { "molecular_weight": 15278.477399999987, "isoelectric_point": 9.711791419982909 }, "id": "NKOCPLAKPK_861", "locus": "GCA003260355_00825" }, { "type": "cds", "sequence": "contig_1", "start": 443677, "stop": 445191, "strand": "+", "frame": 1, "gene": "recG", "product": "ATP-dependent DNA helicase RecG", "db_xrefs": [ "EC:3.6.4.12", "EC:5.6.2.4", "GO:0003676", "GO:0003678", "GO:0005524", "GO:0006281", "GO:0006310", "KEGG:K03655", "SO:0001217", "UniRef:UniRef50_A0A328EGK9", "UniRef:UniRef90_A0A328EGK9" ], "nt": "TTGCCTGAGGAGTTCCTCAAGACCTTCCATTTACTCGATGTGCAGAAAACCATCCTCAATATTCACTATCCTGAGAGCGAATCGTTGAAATCACAGGCGATTCATAGGGTGTTTTTTGATCGTTTGCTAAGAGTTCAGCTTTTTTCTCTGATGAATAGGCAAAGCTATCAAGCTAACAAGAAAACTAGCTCTGAAGCGCAGCAACGGGGGGTGTTGAAGGCGATGCTCCAGCGTCTGCCGTTTGAACTTACGATGGCTCAGAAGAAAGTGATCAAGCAAATCATTGATGATTTTCATAGCGGGAAGCCGATGCTCAGGCTTTTGCAAGGAGATGTAGGGAGCGGGAAAACGATCGTTGCAACGCTCGCGGCTTACTATCTCAAGCAGACTGAACAGGGACAAACGGTATTTCTTGCGCCTCTGGAGGTGCTGGCTCAGCAGCATTATCAAAGTATTGCAAAGCTATTGTTGCCGCTTGGGCTAAGAGTTGAGCTTTTGACGGGGTCGCTGACTAAGGGGCAAAAGGAGAAGATTAAATCGGATCTTCAGCAAGGGAAGATTGATGTTATCATCGGGACGCATGCTTTACTTCAAGAAGGGATCAGTTTTCAGAATTTACAGTATGTGATTATTGATGAACAGCACAAATTTGGCGTAAAGCAGAGAGCGTTTTTCAAAAAGTTTGGAGCACCGCATATTTTGCAGATGTCGGCGACGCCGATTCCGAGGTCGATGGCGATTGCGTTCTTTGGGGAGTTTGATGTGAGTATTATTGATGAATTACCGAAAGGAAGGTTGCCGATCCAGACCAAAATCATCTCTAGCACCGAAGAAAAGAAACTCAAGCCTCGAATCCTTGAGAAAATTAGGAAAAATCAGAAGCTTTTCGTCGTGACGCCGCTTATTCAGGAGAGTGAGAGTCTTGATGAAGTGCAATCTGTCTTGCATGCGTATGAAGAAATTCAGCTGCTTTATCCGGAACTGGAAGGGAAGATTGGTTTACTCCATGGGAAGATGAAACCCAAAGAAAAAGAGGAAGCGATGCAAAATTTTAAGAGTGGGAAGACGATGATTCTCGTTGCTACGACAGTAATTGAAGTAGGAGTAGATGTTCCCGAGGCTACGGTGATGATTATTAAGAATGCTGAGAGATTTGGCTTAGCTCAGCTTCATCAGCTCAGAGGAAGAATCGGAAGATCGGATCTTCAATCGTATTGTTTCCTCCAAACCCCCAAAAAATCGGGGGATAGCTACGAAAGGCTTAGAGCGATGGAACAGACGACGGATGGGTTTAAATTAGCTGAGCTGGATTTACAGAATCGTGGATCTGGGGAGATTTTGGGAACGATGCAGTCTGGAATAAGCGATATTCCGATTGAAATCCTCTCAGATTTGAAGTTTTTGGAGCAGGTGCAGGCTGGGGCATCGTGGCTCTTGCAAAAGTATCCGGATTTGGAGGGATTGCCGAGCTTGCAGAAGTTTTTGCACGAGAAAATTGGTGATATTCTTGCTTAG", "aa": "MPEEFLKTFHLLDVQKTILNIHYPESESLKSQAIHRVFFDRLLRVQLFSLMNRQSYQANKKTSSEAQQRGVLKAMLQRLPFELTMAQKKVIKQIIDDFHSGKPMLRLLQGDVGSGKTIVATLAAYYLKQTEQGQTVFLAPLEVLAQQHYQSIAKLLLPLGLRVELLTGSLTKGQKEKIKSDLQQGKIDVIIGTHALLQEGISFQNLQYVIIDEQHKFGVKQRAFFKKFGAPHILQMSATPIPRSMAIAFFGEFDVSIIDELPKGRLPIQTKIISSTEEKKLKPRILEKIRKNQKLFVVTPLIQESESLDEVQSVLHAYEEIQLLYPELEGKIGLLHGKMKPKEKEEAMQNFKSGKTMILVATTVIEVGVDVPEATVMIIKNAERFGLAQLHQLRGRIGRSDLQSYCFLQTPKKSGDSYERLRAMEQTTDGFKLAELDLQNRGSGEILGTMQSGISDIPIEILSDLKFLEQVQAGASWLLQKYPDLEGLPSLQKFLHEKIGDILA", "aa_hexdigest": "54398bbe9d70ba628364824554271d0a", "start_type": "TTG", "rbs_motif": null, "genes": [ "recG" ], "truncated": "5-prime", "pseudogene": { "start": 443377, "stop": 445191, "observations": { "mutation": [ 443542, 443614 ], "stop": [ 443542, 443614 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EGK9", "query_cov": 1.0, "subject_cov": 0.7379209370424598, "identity": 0.998, "score": 957.0, "evalue": 0.0, "gene_start": 1, "gene_end": 504, "reference_start": 180, "reference_end": 683, "reference_sequence": "MELKTLLRTTPRYLNLLAQNGITTIRDFFNYFPRAYEDRANIKPLNQLIFNDKGITATKGKILKKTVFRRGGRMIYDISFEDELGIQGTISIFNSGYLASKLQEGCRYIIVGKPSFKYGKTIFSHPDIVPSSDSGAFEENYNSGRIFPVYSEMNGIKPGWFAKKIWELRDQIPAVFAEYLPEEFLKTFHLLDVQKTILNIHYPESESLKSQAIHRVFFDRLLRVQLFSLMNRQSYQANKKTSSEAQQRGVLKAMLQRLPFELTMAQKKVIKQIIDDFHSGKPMLRLLQGDVGSGKTIVATLAAYYLKQTEQGQTVFLAPLEVLAQQHYQSIAKLLLPLGLRVELLTGSLTKGQKEKIKSDLQQGKIDVIIGTHALLQEGISFQNLQYVIIDEQHKFGVKQRAFFKKFGAPHILQMSATPIPRSMAIAFFGEFDVSIIDELPKGRLPIQTKIISSTEEKKLKPRILEKIRKNQKLFVVTPLIQESESLDEVQSVLHAYEEIQLLYPELEGKIGLLHGKMKPKEKEEAMQNFKSGKTMILVATTVIEVGVDVPEATVMIIKNAERFGLAQLHQLRGRIGRSDLQSYCFLQTPKKSGDSYERLRAMEQTTDGFKLAELDLQNRGSGEILGTMQSGISDIPIEILSDLKFLEQVQAGASWLLQKYPDLEGLPSLQKFLHEKIGDILA" }, "paralog": false, "identity": 0.9966887417218543, "score": 1172.0, "evalue": 0.0, "description": "Internal stop codon at 443542, 443614. Nonsense mutation around 443542, 443614.", "psc": { "uniref90_id": "UniRef90_A0A328EGK9", "gene": "recG", "product": "ATP-dependent DNA helicase RecG", "ec_ids": [ "3.6.4.12", "5.6.2.4" ], "uniref50_id": "UniRef50_A0A328EGK9", "kegg_orthology_id": "K03655", "go_ids": [ "GO:0003676", "GO:0003678", "GO:0005524", "GO:0006281", "GO:0006310" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EGK9", "EC:3.6.4.12", "EC:5.6.2.4", "UniRef:UniRef50_A0A328EGK9", "KEGG:K03655", "GO:0003676", "GO:0003678", "GO:0005524", "GO:0006281", "GO:0006310" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EGK9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EGK9" ], "product": "ATP-dependent DNA helicase RecG" } }, "id": "NKOCPLAKPK_862", "locus": "GCA003260355_00826" }, { "type": "cds", "sequence": "contig_1", "start": 445239, "stop": 445976, "strand": "+", "frame": 3, "gene": null, "product": "GGDEF domain-containing protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBF3D2B", "UniRef:UniRef100_A0A328EG85", "UniRef:UniRef50_A0A328EG85", "UniRef:UniRef90_A0A328EG85" ], "nt": "ATGTATCTGTATAGTTTCTCCCATTATATTGGGCAGGATAGGGTATTCTGGTGTGAGTTGCCGAGTAAAAAGGTCCAGGCTGGAGATGTCTTGCAATCTGAGTCAGATTTTTTTCTTGTATTAGGAGAGACGGTGAGTTTTCCTGAGCTTTATCAGCTGCGTTATCCGATGCAGGGGGAGTTTCATGTAGCGTTTCCCAAGCTTTTATCAAGTCCTGCACTTGAATTGGTGCATCGGATGGTTTATGAGCGGTATACGACCTATAAAAACACGCTCAAGCTCTTTCTTGACCATGAAATTCAGAATCTGCTTGCTAAAGCAAAGCGTCCTACGAGCAAAAAATACCAGCCTCTTGATTTCAGCATAGGGAAACAGAGGATTCATTCGGAAGAACAGGAACAAATTCTGATTATTTTCCCGGATTTGCGAACGTTTACGAATGTGCTTGATCAGCAAGCTGTCGGCGGACTTTTTCTCAGTGCTTTGGACACTCAAGCCAGGAAAAATCTCAACCGATGGAAAATAAAACAGGGAGAAGAGCAGTTGATCTTTTGCACGGGGGCGGAAGTCTTCCAAGATTTTAAGAAGCTCGGGCAGATTTTCCTTGTTGAGCCACAAAAACGATATTATGCTAGCCAGCAAGACCCGAGATATAAGCTAGAAACGGTTGCGAAAAAGATTGCTGAGCTGCATGGTATCCCTTTACAGTTGATTGAGAGCGAAATGCTCGCTCAATAA", "aa": "MYLYSFSHYIGQDRVFWCELPSKKVQAGDVLQSESDFFLVLGETVSFPELYQLRYPMQGEFHVAFPKLLSSPALELVHRMVYERYTTYKNTLKLFLDHEIQNLLAKAKRPTSKKYQPLDFSIGKQRIHSEEQEQILIIFPDLRTFTNVLDQQAVGGLFLSALDTQARKNLNRWKIKQGEEQLIFCTGAEVFQDFKKLGQIFLVEPQKRYYASQQDPRYKLETVAKKIAELHGIPLQLIESEMLAQ", "aa_hexdigest": "9163a6ec68e28f68b1571b9a1b61182a", "start_type": "ATG", "rbs_motif": "TTTA", "ups": { "uniparc_id": "UPI000DBF3D2B", "uniref100_id": "UniRef100_A0A328EG85", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBF3D2B", "UniRef:UniRef100_A0A328EG85" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EG85", "uniref90_id": "UniRef90_A0A328EG85" }, "psc": { "uniref90_id": "UniRef90_A0A328EG85", "product": "GGDEF domain-containing protein", "uniref50_id": "UniRef50_A0A328EG85" }, "pscc": { "uniref50_id": "UniRef50_A0A328EG85", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EG85" ], "product": "GGDEF domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_863", "locus": "GCA003260355_00827" }, { "type": "cds", "sequence": "contig_1", "start": 445982, "stop": 446383, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGAGCGGTATATTTCAACGTGGATTATACGAATGGTTTGACTTTGCCGTTAGTAGGCGAGGCAGATCGAGCAATTATCAATACAACGATAGGGAAATTTTATGATGGATTCTGGGATCTTTATAGTAGTTCTAAATCAGATTTTTCATCGCTTTTAGCTTTATTCGGTTTAGTAAAAGTCGAGATTAAAGGCAAGGAAACTTTGCTGCCAAGAATGGTCGCAAAACAAATTCCTATCATCGAAAAAATCATCAACGACAAATTTAGCGACGACCCACAAGGAAAATCAGACTTATACTCCCAATTACTCAAAGCGCCGAGCGGAGAAGACTCTTTTGATCAAGCAATACAAGTATTATCCGAGCACTATATGCTCCAAGGCAGCGGAACTGCCGAGTAA", "aa": "MGAVYFNVDYTNGLTLPLVGEADRAIINTTIGKFYDGFWDLYSSSKSDFSSLLALFGLVKVEIKGKETLLPRMVAKQIPIIEKIINDKFSDDPQGKSDLYSQLLKAPSGEDSFDQAIQVLSEHYMLQGSGTAE", "aa_hexdigest": "bc34d1910c275144fb01f7c7f0160cf6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14676.49649999998, "isoelectric_point": 4.7006643295288075 }, "id": "NKOCPLAKPK_864", "locus": "GCA003260355_00828" }, { "type": "cds", "sequence": "contig_1", "start": 446444, "stop": 446653, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGACCTTCTATAATTGGGGCAAGGCAACGAGCAACAGACTACGAAAAACTCCAACACAAATCCTCAATGATCCAGAATGGAACACCCTAACGAGACTAAAAGCACTCGGAAAACCACTTTTCATTGATGAAGTGGGGACAACAGCAGTTTGGTATGGCGGGAAATATCAAGCCGACACCTCAAGAGCGGAATTCCTCTCTGAAACCTGA", "aa": "MTFYNWGKATSNRLRKTPTQILNDPEWNTLTRLKALGKPLFIDEVGTTAVWYGGKYQADTSRAEFLSET", "aa_hexdigest": "8617dc32253ae11e51f171eef767e466", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7899.816200000001, "isoelectric_point": 9.15555820465088 }, "id": "NKOCPLAKPK_865", "locus": "GCA003260355_00829" }, { "type": "cds", "sequence": "contig_1", "start": 446858, "stop": 447376, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAGTCTGGATTTTGATGCTATTTTTCCTCGCATTCCCTTTCTCAACATCTGCTTTCGTTCTGGGACTAAAATCTACGGATAATACGGTGGAAAACGTAGTAAAGATAGAAAAAGACTATAGCCTCTATCTGCCTTTGATTGGCTTTATTTACGATCCACGAGACAAGCAAGACGTAGTAGCTGAGCTGGAGAAAATAGCAAAAGAGCTGGGAAACCAAAGAATCTATCATATCTCGCTTTCTCCAAACCAATTTTCTGCTGAAGAGGTAGCCAAAGGAGCCTTTGACGAACAATACACCCTATTTTTCAAGACAATCAAGAGACTAAATTTGAAAGTAATCTTCAGGACGATGCATGAAATGAATGGCGGTTGGTATCCACGAGGCTCAAACCCCACAAAATTCAAAGAAGCATGGATACATGTATGGAATTTAGCACGTTCGCTGGGATTGAATCAGCAAGATATCCTGTTTGACTTCTCTGTGAATCACCGAGATATGCCAACCCTCTGA", "aa": "MKKVWILMLFFLAFPFSTSAFVLGLKSTDNTVENVVKIEKDYSLYLPLIGFIYDPRDKQDVVAELEKIAKELGNQRIYHISLSPNQFSAEEVAKGAFDEQYTLFFKTIKRLNLKVIFRTMHEMNGGWYPRGSNPTKFKEAWIHVWNLARSLGLNQQDILFDFSVNHRDMPTL", "aa_hexdigest": "a125573e7ea9b57b041837331143f4a5", "start_type": "ATG", "rbs_motif": "TTTA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 20071.995499999994, "isoelectric_point": 8.731291770935059 }, "id": "NKOCPLAKPK_866", "locus": "GCA003260355_00830" }, { "type": "cds", "sequence": "contig_1", "start": 447566, "stop": 447835, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAACTTCTTATTCAAACCCCATTATATCATGAAAATAAATATCTCCCTCACCTTTTAGAGCATTGTGTTCTCTATAGTCAACATGATGAAGAGCTTCTTTTCCTTCCGGATATTTTTGCTTCAACGAGAACAGGATATACCAGTTTCGAGTGGAAAAATATGTCGCTAGAAAAGATTCTTTTCTTTTTAGAAAGACCAGTAGATGAAAATATCTTTTTTTTACAGCAAAAAGTCGTAAAAAATGAGCTTAATAATTCCTCATTTTGA", "aa": "MELLIQTPLYHENKYLPHLLEHCVLYSQHDEELLFLPDIFASTRTGYTSFEWKNMSLEKILFFLERPVDENIFFLQQKVVKNELNNSSF", "aa_hexdigest": "b51fc928b30fa6dc8c2bd0f5ebf5ad2c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10725.15579999999, "isoelectric_point": 5.027090644836425 }, "id": "NKOCPLAKPK_867", "locus": "GCA003260355_00831" }, { "type": "cds", "sequence": "contig_1", "start": 448811, "stop": 449068, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00070.33", "PFAM:PF07992.20" ], "nt": "TTGACGCACTTTGCAAGCGAAGTACTTTTGCTTGTCAGAAGAGATCAATTGAGAGCATCAAAGGCAATGCAAGAAAAAGCCTTTAAAAACGAAAAAATTCAGATTCTTTGGAATACAGAAGCTACTGAACTTGTAGGAGAAAAACTTTTATCTGGAGTTTGGACAATCAACAACAAGACGCAAGAAAAGGTTTTGATTGAATGTGCCGGAGTTTTTTATGCTATTGGTCATACTCCAAATACTACTTTCTTAGGCTGA", "aa": "MTHFASEVLLLVRRDQLRASKAMQEKAFKNEKIQILWNTEATELVGEKLLSGVWTINNKTQEKVLIECAGVFYAIGHTPNTTFLG", "aa_hexdigest": "425af107a7259f94cbdf1ff79a23fb2b", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00070.33", "name": "Pyr_redox", "length": 53, "aa_cov": 0.5529411764705883, "hmm_cov": 0.9811320754716981, "evalue": 1.117296444289386e-07, "score": 28.7388973236084, "start": 2, "stop": 48 }, { "id": "PF07992.20", "name": "Pyr_redox_2", "length": 89, "aa_cov": 0.9529411764705882, "hmm_cov": 0.9101123595505618, "evalue": 1.1590391513350347e-09, "score": 34.490989685058594, "start": 3, "stop": 83 } ], "seq_stats": { "molecular_weight": 9621.039499999992, "isoelectric_point": 7.902937507629395 }, "id": "NKOCPLAKPK_868", "locus": "GCA003260355_00832" }, { "type": "cds", "sequence": "contig_1", "start": 449336, "stop": 449569, "strand": "-", "frame": 3, "gene": null, "product": "FAD-dependent oxidoreductase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00286AA1A5" ], "nt": "ATGCATCGTAAAGTAATTATTATTGGTTCTGGTCCTGCGGGACATACTGCTGCTATTTATGCGGGGAAGGCGCTTTTGCAGCCACTCATGTTTGAAGGTTTTATGGCAGGAGGAATTGCTGCTGGCGGGCAGCTCACCACTACAACGGTGATAGAAAACTTCCCTGGATTCCCTGAGGGGATTGATGGAAGTCAACTCATGATGCAGATGAGGCAGCAATCTCTCAATGCCTGA", "aa": "MHRKVIIIGSGPAGHTAAIYAGKALLQPLMFEGFMAGGIAAGGQLTTTTVIENFPGFPEGIDGSQLMMQMRQQSLNA", "aa_hexdigest": "4f4352de6af9cebaa37170220944dee0", "start_type": "ATG", "rbs_motif": "TTATT", "psc": { "uniref90_id": "UniRef90_UPI00286AA1A5", "query_cov": 0.8701298701298701, "subject_cov": 0.8481012658227848, "identity": 0.701, "score": 97.4, "evalue": 4.3e-25, "valid": false, "product": "FAD-dependent oxidoreductase", "uniref50_id": "UniRef50_UPI00286AA1A5" }, "pscc": { "uniref50_id": "UniRef50_UPI00286AA1A5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00286AA1A5" ], "product": "FAD-dependent oxidoreductase" }, "genes": [], "id": "NKOCPLAKPK_869", "locus": "GCA003260355_00833" }, { "type": "cds", "sequence": "contig_1", "start": 450624, "stop": 450905, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00575.29" ], "nt": "ATGAAACTTCAAGGCGTAGTAAGAAATGTGACGGATTTTGGTGCCTTTGTAGATGTTGGGTTGCATAATGACGGGCTCGTGCATAAATCGCAGATGGCGGACTATTTCGTAACCAATCCGCTTGAGGTTGTGCATGTCGGACAGCAGATAGAGGTCAGGGTTTTGGAAATTGATAAAGAAAGAGAAAAGCTTTCCCTTTCTATGAAGGGTGAGCATAATTCCAAGCCTCCTCAGAGGAAAACTCAGCCCCGCCCAGAAAAAAGAGGGGAGTCTGGAGCGTGA", "aa": "MKLQGVVRNVTDFGAFVDVGLHNDGLVHKSQMADYFVTNPLEVVHVGQQIEVRVLEIDKEREKLSLSMKGEHNSKPPQRKTQPRPEKRGESGA", "aa_hexdigest": "38d44cb4d87e201ae4efaccd981d0a98", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00575.29", "name": "S1", "length": 67, "aa_cov": 0.7204301075268817, "hmm_cov": 1.0, "evalue": 9.759588508430542e-21, "score": 70.44349670410156, "start": 3, "stop": 69 } ], "seq_stats": { "molecular_weight": 10462.79099999999, "isoelectric_point": 8.086285591125488 }, "id": "NKOCPLAKPK_870", "locus": "GCA003260355_00834" }, { "type": "cds", "sequence": "contig_1", "start": 451026, "stop": 452858, "strand": "-", "frame": 2, "gene": null, "product": "S1 motif domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EKL8", "UniRef:UniRef90_A0A328EKL8" ], "nt": "ATGACGACTTTACTCCAACAATTACAGCAGAAAACTGGTATGGAAGCTGCGGTAATCAACAATATTATTCAGCTTTTGGATGAAGGGAACACAATACCGTTTATTGCGCGTTATCGTAAGGAAATGACAAAGGGGGCTACCGACGAACAGCTTCGTGATTTTTTTGAACTCTACAGTTATCGTAAAAATTTGGAGGCAAGGAAGGAAGACGTGATCCGTTTGATTGCGGAGAGGGGACTCTTGACCGAAAGTCTGAAAAATCAGATTTTAGCTGCAGAGACGCTTGCGAGAGTGGAAGATCTTTATAGACCTTTCAAGGAAAAGAAGAATACCAAAGCAACTATCGCCAAAGCGAAGGGCTTGGAGCCGCTAGCAAAGCTCTTATCAACCGCAGAGCTTACAAAAGAAGAGTTTGAAAGCAGAGCCGATACCTTTATTAAGGATACGGGCGACGAAAAGACCTCAGTGAAGGATCGTGCAGAGGCTATTCAGGGAGCAAAGGATATTGTTGCAGAAGAGGTATCAGATCACGCAGATTTAAGAGCCGACATCAAGCAATTTGAAGAACAAAACGCCACATTAGAGACCAAAGCGACCAAGACTTTTGAGGAAAATGGGGTATACAGGGTATATCAGCAGTATCAGAAGAAACTTTTTGAAATGCCTTCCTATGCCTATCTGGCGGTAAGTAGAGCGGAGAAAGAAAAGCAGCTCAGCATCAAATTGCAGTTTTCTCAGCAGAAAATCTCGGAGTATGCAAGCAAGTATTTTATTCCTAGCGTTGCGAACAGCTCGGTTGAGTATTTACAGCAAGCAATTGACGATGGACTGGAGAGATTACTCCTGCCTTCACTAGAAAGAGAGCTAAGAGCCGACAAAAAGAGGCGAGCGGACGAGGCTGCAATCAAAGTCTTTGGAGATAATTTGAAGCAGCTCCTTTTGACGCCACCGGTAAAAGGGTTGACGGTATTGGGATTTGATCCTGCGTTTAGAACCGGTTGCAAGCTTGCGGTCGTCGATCAAACGGGGAAATTCTTAGCGAAAGAGGTGATTTATCCTACTGAACCACAAAAAAAGATTGCAGAAGCAGAAAAGACCCTACTTGATTTGGTGCAGAAGTATGGGATTCAGCTGATTGTGATAGGAAACGGGACAGCATCTAGGGAGTCGGAGAAAGTGGTAGCAGATTTTATCAAAAAATATATGCCAACGCTTCAATATGTGATTACTTCTGAGGCGGGGGCGTCAGTATATTCGGCGTCGGAGCTTGCACAGCAGGAATATCCACAGCTTGATGTGACGATTAGAGGAGCGATTAGTATTGCTCACAGAGTGCAGGATCCTTTGGCAGAGTTGACCAAAATTGATCCTAAAGCGATCGGAGTAGGGCAGTATCAGCACGATGTAGATCAGAAATTTCTCAAAGAGAAGCTAAATGAGAAGGTAGAAGATGTGGTAAACGCAGTTGGAGTAGATGTAAACACGGCAAGCTATACGCTTTTGCAGTATATTGCAGGGTTATCGGCCTCAGTTGCCAAGAATGTAGTAGCCTATAGAGATGAAAATGGGTCTTTTACCGATAAAAAGCAGATCAAGAAAGTGAAAGGGCTTGGACCTAAGGCATATGAGCAGGCAGTAGGATTTCTTAGGATCAAAGAAGGGAAAGAGCCACTTGATGCGACGGGGATTCATCCTGATCATTATGCAGCAACCTTGAGTTTTCTGGAAGGGGAGTTGGGTATCAAAAAGAAAAATCTGATTCTTCCCCTTCAGTCCCTCAAAAACTACAGCGAACAAGAAATTGAAGGGTTTGCCAACAAATACGGACTTTGA", "aa": "MTTLLQQLQQKTGMEAAVINNIIQLLDEGNTIPFIARYRKEMTKGATDEQLRDFFELYSYRKNLEARKEDVIRLIAERGLLTESLKNQILAAETLARVEDLYRPFKEKKNTKATIAKAKGLEPLAKLLSTAELTKEEFESRADTFIKDTGDEKTSVKDRAEAIQGAKDIVAEEVSDHADLRADIKQFEEQNATLETKATKTFEENGVYRVYQQYQKKLFEMPSYAYLAVSRAEKEKQLSIKLQFSQQKISEYASKYFIPSVANSSVEYLQQAIDDGLERLLLPSLERELRADKKRRADEAAIKVFGDNLKQLLLTPPVKGLTVLGFDPAFRTGCKLAVVDQTGKFLAKEVIYPTEPQKKIAEAEKTLLDLVQKYGIQLIVIGNGTASRESEKVVADFIKKYMPTLQYVITSEAGASVYSASELAQQEYPQLDVTIRGAISIAHRVQDPLAELTKIDPKAIGVGQYQHDVDQKFLKEKLNEKVEDVVNAVGVDVNTASYTLLQYIAGLSASVAKNVVAYRDENGSFTDKKQIKKVKGLGPKAYEQAVGFLRIKEGKEPLDATGIHPDHYAATLSFLEGELGIKKKNLILPLQSLKNYSEQEIEGFANKYGL", "aa_hexdigest": "fd3e6b247b54c084de394ea3d5b06937", "start_type": "ATG", "rbs_motif": "TTTA", "psc": { "uniref90_id": "UniRef90_A0A328EKL8", "query_cov": 1.0, "subject_cov": 0.8100929614873837, "identity": 0.995, "score": 1140.0, "evalue": 0.0, "valid": true, "product": "S1 motif domain-containing protein", "uniref50_id": "UniRef50_A0A328EKL8" }, "pscc": { "uniref50_id": "UniRef50_A0A328EKL8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EKL8" ], "product": "S1 motif domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_871", "locus": "GCA003260355_00835" }, { "type": "cds", "sequence": "contig_1", "start": 453074, "stop": 453268, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DC04964", "UniRef:UniRef100_A0A660MXR4", "UniRef:UniRef50_A0A2W4KDQ4", "UniRef:UniRef90_A0A660MXR4" ], "nt": "ATGACCGACACCAAAGCAACCCAAGAAAAGATCTCTAAAAGTCTGGAAAATCCGATTTTGGAGGCGGTGAAGAAGGACGACCTGAGCCTCGTGATGAAAAATTCAGCTCAGAAGAAAGTTATCCCGCCGAATCAAATATCCGTAGCACTCGACAAGTTTGAGAATAAGATTATCGAGGAGCATCGAGAACATTAG", "aa": "MTDTKATQEKISKSLENPILEAVKKDDLSLVMKNSAQKKVIPPNQISVALDKFENKIIEEHREH", "aa_hexdigest": "a94a30796802945bab9e477d0f46f365", "start_type": "ATG", "rbs_motif": "TTTTT", "ups": { "uniparc_id": "UPI000DC04964", "uniref100_id": "UniRef100_A0A660MXR4", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DC04964", "UniRef:UniRef100_A0A660MXR4" ] }, "ips": { "uniref100_id": "UniRef100_A0A660MXR4", "uniref90_id": "UniRef90_A0A660MXR4" }, "psc": { "uniref90_id": "UniRef90_A0A660MXR4", "uniref50_id": "UniRef50_A0A2W4KDQ4" }, "pscc": { "uniref50_id": "UniRef50_A0A2W4KDQ4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4KDQ4" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7300.327, "isoelectric_point": 6.724132347106934 }, "id": "NKOCPLAKPK_872", "locus": "GCA003260355_00836" }, { "type": "cds", "sequence": "contig_1", "start": 453368, "stop": 453682, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBFDA82", "UniRef:UniRef100_A0A328EK26", "UniRef:UniRef50_A0A328EK26", "UniRef:UniRef90_A0A328EK26" ], "nt": "ATGAGCTGGAAATTAACTATTGACGTGATCTTTAACAGCTTTCCTCTTATAAAAAACAAAAATCTGATCCCTTTTCTCTGTCATTGCAAGCGAAGCGTGGCAATCCAACGACTTCAAACCCGTTATTGTGAGCGAAGTGAAGCAATCCAACGACTTCAAACCGTCATTGCGAACAAAGTGAAGCAATCCACTGACTTCAAACCCGTCATTGCGAGCGAAGCAATGATTTCAAAGGGGCTGTGGAGAAAAAATCCCGCGAATCAAACGTTTCAAGGGGTATATGAACTTTTCAATGCAAGCATTGGAAGTTTTTAG", "aa": "MSWKLTIDVIFNSFPLIKNKNLIPFLCHCKRSVAIQRLQTRYCERSEAIQRLQTVIANKVKQSTDFKPVIASEAMISKGLWRKNPANQTFQGVYELFNASIGSF", "aa_hexdigest": "eb647e21a8614e69e2691399fdc1ae68", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000DBFDA82", "uniref100_id": "UniRef100_A0A328EK26", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBFDA82", "UniRef:UniRef100_A0A328EK26" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EK26", "uniref90_id": "UniRef90_A0A328EK26" }, "psc": { "uniref90_id": "UniRef90_A0A328EK26", "uniref50_id": "UniRef50_A0A328EK26" }, "pscc": { "uniref50_id": "UniRef50_A0A328EK26", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EK26" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11966.874399999992, "isoelectric_point": 9.959478950500486 }, "id": "NKOCPLAKPK_873", "locus": "GCA003260355_00837" }, { "type": "cds", "sequence": "contig_1", "start": 454126, "stop": 454245, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACGCATGCAGAAGAAGTAAAAGAGTTTTCTCCCAAGAAACTCACGAATGCCAACTATGGTGCCTTTATGCAAGCCTTTGAAAAGCTTGTAGCGAAGGGGACGACGGAAAAACTTTGA", "aa": "MTHAEEVKEFSPKKLTNANYGAFMQAFEKLVAKGTTEKL", "aa_hexdigest": "77d3365cfb5e32c3587b7c0fc63ecfd9", "start_type": "ATG", "rbs_motif": "TTCTT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4389.014400000001, "isoelectric_point": 8.087252616882324 }, "id": "NKOCPLAKPK_874", "locus": "GCA003260355_00838" }, { "type": "cds", "sequence": "contig_1", "start": 454312, "stop": 454575, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCACCAAGAAGAATTTACCAAAGCTTTGCAGGAGCTGGACAAAAAACGTGATCAGCTTCTGAGTTTTATCTTTGCTAAGATTAGTATTGAGGCACAGTCCCCTGATACGCAAGTGCAGAAGGCTGGGCAACTTTTACTCCCGCTGAAAAAGCAATATCAAGGGATCCAGACCAAGCAAGACCGTGCAGAGAGCTTTTTGATCAGCGGTTTGCTCGTGGACCTCGAGAAAACCGAGCATGCTGCGGCAGTAGCCAAAATCTGA", "aa": "MHQEEFTKALQELDKKRDQLLSFIFAKISIEAQSPDTQVQKAGQLLLPLKKQYQGIQTKQDRAESFLISGLLVDLEKTEHAAAVAKI", "aa_hexdigest": "791957e11058b4e31116d409e184a5a6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9837.226199999996, "isoelectric_point": 6.7253828048706055 }, "id": "NKOCPLAKPK_875", "locus": "GCA003260355_00839" }, { "type": "cds", "sequence": "contig_1", "start": 454588, "stop": 454929, "strand": "+", "frame": 1, "gene": null, "product": "Toxic anion resistance protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EGL6", "UniRef:UniRef90_A0A328EGL6" ], "nt": "GTGCAGGAGCTGAAAACGGTGAATGCCAAGTTTATCGAAACGATGGCAGAACGCTCTGAAAAGGAGATTGTTAGGAATCTGCAAGGAGCGAAGAAACTCCGTGAGGAGTTAGATACGCTCTACAGGCAGTTCATCAGGAAAATTGACGCACTTGGGGTCGTAAGTCCGAATCCAGAGCTGGTGGCGTGCATTGCTTCGCTCAATCAACTCATTCAAGAGACACGTAATGCCCGAACCATCCATAAAGGGTTGGTGGGAGCCTATACGACGGACGGAGAATGGGAAGATGAAGAAAAACCTGAAGCTGAAAGCGTTGCCTCCGAAGCAACAGATAAAGCCTAA", "aa": "MQELKTVNAKFIETMAERSEKEIVRNLQGAKKLREELDTLYRQFIRKIDALGVVSPNPELVACIASLNQLIQETRNARTIHKGLVGAYTTDGEWEDEEKPEAESVASEATDKA", "aa_hexdigest": "35db75f5fdd65f7c0430aa1da721593e", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 454288, "stop": 454929, "observations": { "mutation": [ 454573 ], "stop": [ 454573 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EGL6", "query_cov": 1.0, "subject_cov": 0.4232209737827715, "identity": 0.973, "score": 207.0, "evalue": 1.78e-65, "gene_start": 1, "gene_end": 113, "reference_start": 155, "reference_end": 267, "reference_sequence": "MTHAEEVKEFSPKKLTNANYGAFMQAFEKLVAKGTTEKLGLEASDFTAFQDKLKVFLDLNQEMHQEEFTKALQELDKKRDQLLSFIFAKISIEAQSPDTQVQKAGQLLLPLKKQYQGIQTKQDRAESFLISGLLVDLEKTEHAAAVAKIGLTAGVQELKTVNAKFIETMAERSEKEIVRNLQGAKKLREELDTLYRQFIRKIDALGVVSPNPELVACIASLNQLIQETRNARTIHKGLVGAYTTDGEWEDEEKPEAESVSSEETDKA" }, "paralog": false, "identity": 0.9859154929577465, "score": 402.0, "evalue": 9.51e-145, "description": "Internal stop codon at 454573. Nonsense mutation around 454573.", "psc": { "uniref90_id": "UniRef90_A0A328EGL6", "product": "Toxic anion resistance protein", "uniref50_id": "UniRef50_A0A328EGL6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EGL6", "UniRef:UniRef50_A0A328EGL6" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EGL6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EGL6" ], "product": "Toxic anion resistance protein" } }, "id": "NKOCPLAKPK_876", "locus": "GCA003260355_00840" }, { "type": "cds", "sequence": "contig_1", "start": 455083, "stop": 455544, "strand": "+", "frame": 1, "gene": null, "product": "DUF5079 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBF5A6B", "UniRef:UniRef100_A0A328EG94", "UniRef:UniRef50_A0A328EG94", "UniRef:UniRef90_A0A328EG94" ], "nt": "ATGGAGAATCATATTGATCAAATCGGTGTTTATGACAGTGGTCCAGATTTTGGGGGGGTAGGTCCATTCCTCTTTGTGCTCATCCTCATTGCTGTCTGGAATATGCTTATTTTTGGGGTGTTGAGAGCCATAATGAGGATTAAAGATAGTGGAAAAGCTCTCAAGTTGAGAATTATGTGGCTCTCAGTACTTTCTTTTGTTGCCATGATGGGTATTACAGGGGGAAATCAATTACTTCCCACCATAGTAAAGGCCGCTTCTACTAATGAAATACTCCTCTACTGTGGTATTTTTTTGATTTGTGAGGTTTTATCCTTTATCATTATCAGAAAATGGATTATCTCCAAAATGGAAATCTCCTCAATCAAGAAGCTATGGCTTAGCCTTGGACTTGTGCTCCTTAACCTTCCTACCTTCTTTGGAATCGTTTTCTTTATTAATAGTCTACCTTACTTTCACTAA", "aa": "MENHIDQIGVYDSGPDFGGVGPFLFVLILIAVWNMLIFGVLRAIMRIKDSGKALKLRIMWLSVLSFVAMMGITGGNQLLPTIVKAASTNEILLYCGIFLICEVLSFIIIRKWIISKMEISSIKKLWLSLGLVLLNLPTFFGIVFFINSLPYFH", "aa_hexdigest": "9b3d36c226ed2d1b2128ba4c564b4823", "start_type": "ATG", "rbs_motif": "TTCTT", "ups": { "uniparc_id": "UPI000DBF5A6B", "uniref100_id": "UniRef100_A0A328EG94", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBF5A6B", "UniRef:UniRef100_A0A328EG94" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EG94", "uniref90_id": "UniRef90_A0A328EG94" }, "psc": { "uniref90_id": "UniRef90_A0A328EG94", "product": "DUF5079 family protein", "uniref50_id": "UniRef50_A0A328EG94" }, "pscc": { "uniref50_id": "UniRef50_A0A328EG94", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EG94" ], "product": "DUF5079 family protein" }, "genes": [], "id": "NKOCPLAKPK_877", "locus": "GCA003260355_00841" }, { "type": "cds", "sequence": "contig_1", "start": 456239, "stop": 456400, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCCTGCATTCAGGAAATCTTTGCTACAAGACTAAGAACGAGGGCATTCCATCCGAAAGTTATAATTCATAATACCACTAATCCAAGCCATGGATATAAAAACATTATTGCGACAATATACGCTATTAGTTGTAGAATTGTTTCTCCCATCCAACTATAA", "aa": "MPCIQEIFATRLRTRAFHPKVIIHNTTNPSHGYKNIIATIYAISCRIVSPIQL", "aa_hexdigest": "2a6d92dbe7b6f50c234e88e14284fe36", "start_type": "ATG", "rbs_motif": "TTTTTA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6049.104899999999, "isoelectric_point": 10.05199108123779 }, "id": "NKOCPLAKPK_878", "locus": "GCA003260355_00842" }, { "type": "cds", "sequence": "contig_1", "start": 456477, "stop": 456707, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EGV9", "UniRef:UniRef90_A0A328EGV9" ], "nt": "ATGACGATTGTTTGGGGGGTGGCAGATGAAAATCCAGAAGCTTCGATGTGGTTATTCTATGCAATATCCATTGGTATACGAATTCTTATCGCATTTATCAATAAAAATCGGATGCTTTCAGGGTGTGAACTCGATCCGACAAAGAAGAATTGGCTAGCTCTAGGGACGGCTTTCTTGAGTTTCCCAATGCTCTTTCTGATTTTTTTATTTGTAATGGAAACCTTTTTGTAA", "aa": "MTIVWGVADENPEASMWLFYAISIGIRILIAFINKNRMLSGCELDPTKKNWLALGTAFLSFPMLFLIFLFVMETFL", "aa_hexdigest": "d0c1253a9d76f3901b00b596e0fffaea", "start_type": "ATG", "rbs_motif": "TTTA", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 456249, "stop": 456707, "observations": { "mutation": [ 456330, 456387, 456471 ], "stop": [ 456330, 456387, 456471 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EGV9", "query_cov": 1.0, "subject_cov": 0.5, "identity": 1.0, "score": 153.0, "evalue": 2.95e-46, "gene_start": 1, "gene_end": 76, "reference_start": 77, "reference_end": 152, "reference_sequence": "MGETILQLIAYIVAIMFLYPWLGLVVLGIITFGWNALVLSLVAKISGMQGIKTLLKKRFFKFIGVRFLENLGTLGAMTIVWGVADENPEASMWLFYAISIGIRILIAFINKNRMLSGCELDPTKKNWLALGTAFLSFPMLFLIFLFVMETFL" }, "paralog": false, "identity": 0.9802631578947368, "score": 291.0, "evalue": 3.8e-103, "description": "Internal stop codon at 456330, 456387, 456471. Nonsense mutation around 456330, 456387, 456471.", "psc": { "uniref90_id": "UniRef90_A0A328EGV9", "uniref50_id": "UniRef50_A0A328EGV9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EGV9", "UniRef:UniRef50_A0A328EGV9" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EGV9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EGV9" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 8722.391399999997, "isoelectric_point": 5.05215663909912 }, "id": "NKOCPLAKPK_879", "locus": "GCA003260355_00843" }, { "type": "cds", "sequence": "contig_1", "start": 457115, "stop": 457300, "strand": "+", "frame": 2, "gene": null, "product": "GtrA-like protein domain-containing protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DC050CE", "UniRef:UniRef100_A0A328EJV6", "UniRef:UniRef50_A0A328EJV6", "UniRef:UniRef90_A0A328EJV6" ], "nt": "ATGATTGCTTCAGTAGCTTCAGGAATTTTTATTTATTTTTTGAACAAAAAATGGACTTTCTCAAAGACAGAACTCACTTCTGAGCAAAAAGAAAAATTTTGTCGCTATCTTGCATACTTTAGTTTTCCTCATGTCATAATCCCATTACTCGTAATTGCTTATTTCGTTTTTTTATTGCTTCTCTAG", "aa": "MIASVASGIFIYFLNKKWTFSKTELTSEQKEKFCRYLAYFSFPHVIIPLLVIAYFVFLLLL", "aa_hexdigest": "af7585181e1170fe3e1b7b0966294d5c", "start_type": "ATG", "rbs_motif": "TTATT", "ups": { "uniparc_id": "UPI000DC050CE", "uniref100_id": "UniRef100_A0A328EJV6", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DC050CE", "UniRef:UniRef100_A0A328EJV6" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EJV6", "uniref90_id": "UniRef90_A0A328EJV6" }, "psc": { "uniref90_id": "UniRef90_A0A328EJV6", "product": "GtrA-like protein domain-containing protein", "uniref50_id": "UniRef50_A0A328EJV6" }, "pscc": { "uniref50_id": "UniRef50_A0A328EJV6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EJV6" ], "product": "GtrA-like protein domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_880", "locus": "GCA003260355_00844" }, { "type": "cds", "sequence": "contig_1", "start": 457334, "stop": 457648, "strand": "+", "frame": 2, "gene": null, "product": "Sodium-dependent transporter", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MVT1", "UniRef:UniRef90_A0A328EK01" ], "nt": "ATGTTTGAAGAAATCCTCGGCTTATTCTTCGGCTTATTCTTAGGAGAAGCTCCAGATCTTTTAACTTTTATAACGCTACTCTTGGTTGCTATCCTTCGAAATCTCATCCTTCTAAGGATAGTTATCAAAAAAACAACAAAGCCTGAGAATCAAAAAATACTTAAACAAGGAAAAACTCCTGTTTTAGATACTAAAAAGCTTTTTAAGCAGACAGCTTGGAAAAGTATAGGATTTCGGCTCTTAGCAATGGTCATAGCTGTGGGAGCAACATGTTATTCTCCCCTTATTACTAATATGTTATATAACTTCAGTTGA", "aa": "MFEEILGLFFGLFLGEAPDLLTFITLLLVAILRNLILLRIVIKKTTKPENQKILKQGKTPVLDTKKLFKQTAWKSIGFRLLAMVIAVGATCYSPLITNMLYNFS", "aa_hexdigest": "5a4080e257201f4873a0ddee8625c57c", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 457334, "stop": 457870, "observations": { "mutation": [ 457646, 457688 ], "stop": [ 457646, 457688 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EK01", "query_cov": 1.0, "subject_cov": 0.5842696629213483, "identity": 1.0, "score": 194.0, "evalue": 1.11e-61, "gene_start": 1, "gene_end": 104, "reference_start": 1, "reference_end": 104, "reference_sequence": "MFEEILGLFFGLFLGEAPDLLTFITLLLVAILRNLILLRIVIKKTTKPENQKILKQGKTPVLDTKKLFKQTAWKSIGFRLLAMVIAVGATCYSPLITNMLYNFSGHEEFFGPLRYIIRGAIITFPTACGVMIYLFNKKWTFSGTELTLEQKKKISRYLAYFSFPHVIIRPLVVLSIMR" }, "paralog": false, "identity": 0.9887640449438202, "score": 345.0, "evalue": 9.46e-124, "description": "Internal stop codon at 457646, 457688. Nonsense mutation around 457646, 457688.", "psc": { "uniref90_id": "UniRef90_A0A328EK01", "uniref50_id": "UniRef50_A0A660MVT1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EK01", "UniRef:UniRef50_A0A660MVT1" ] }, "pscc": { "uniref50_id": "UniRef50_A0A660MVT1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MVT1" ], "product": "Sodium-dependent transporter" } }, "id": "NKOCPLAKPK_881", "locus": "GCA003260355_00845" }, { "type": "cds", "sequence": "contig_1", "start": 457724, "stop": 457870, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTTATCTCTTCAATAAAAAATGGACATTTTCTGGGACAGAACTCACTCTTGAACAGAAAAAGAAGATTTCTCGTTATCTTGCATATTTTAGTTTTCCTCACGTGATAATCCGACCTCTCGTCGTCCTTAGTATCATGCGGTAG", "aa": "MIYLFNKKWTFSGTELTLEQKKKISRYLAYFSFPHVIIRPLVVLSIMR", "aa_hexdigest": "0a28c538a8a166ef78f8e4781c1678ee", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5807.956799999999, "isoelectric_point": 10.21090564727783 }, "id": "NKOCPLAKPK_882", "locus": "GCA003260355_00846" }, { "type": "cds", "sequence": "contig_1", "start": 458476, "stop": 459153, "strand": "+", "frame": 1, "gene": null, "product": "Glycosyl transferases group 1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4IJP5", "UniRef:UniRef90_A0A328EGP3" ], "nt": "ATGAATACCACAAAAACTCTTGGGCTTCTCTTTCTCGGCAGGCTAGAAAAAGAAAAAGGATTTGATTTGATCTATGATTTTATCAGTCAGTATCCGGGCAAGGAGCTTCCTTTTGATCGATACATTTTTGGCAGCGGAAGCTATGAAAGCGGGATATTAGAGCTGAGTTATCATTTCAAGCAGATTCATTTCTTTGGGTGGAAGCCGCTCTCAGAGGTCGAGAGATACCTTGAAAACATAGATTACTGTCTGATGCCGTCCAGATTCCTCGAGACCTTTGGTTTGAGCGCGATCAACATCCTCCAGCAAGGTATTCCGATTGTTGGTTTCAAAAAAGGTGGGCTGATCCCGTTTATCCAAGATGAATATGCAATTGAACAAAGCGAAGGGAGCACAGATCTTGCAAAGTTTTCTAATATGCTCATCAAACTCCAACAAGAAAAAAAGAAAAAGAAATCTGACTTTTACGAACAGTTGGCTCAGCAGTCCAAGGATATTGCAAATCGCTATACCGTAGAAAAACGATATGAACGCTTTCTTTCCCTTACCGTAACCAAAAAACCTCAAAAGATTGTGCTTGTAAGCGACTTTATAAATAAGATTGGAGGGATTGAAACCTATCTCCATGATACCAAGCATCTTTTGCAGCAATACGGTCACCAAGTAAAACTCTTCTGA", "aa": "MNTTKTLGLLFLGRLEKEKGFDLIYDFISQYPGKELPFDRYIFGSGSYESGILELSYHFKQIHFFGWKPLSEVERYLENIDYCLMPSRFLETFGLSAINILQQGIPIVGFKKGGLIPFIQDEYAIEQSEGSTDLAKFSNMLIKLQQEKKKKKSDFYEQLAQQSKDIANRYTVEKRYERFLSLTVTKKPQKIVLVSDFINKIGGIETYLHDTKHLLQQYGHQVKLF", "aa_hexdigest": "cbaa57b2ef12283950e912ae8f342352", "start_type": "ATG", "rbs_motif": "TTTA", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 458476, "stop": 459456, "observations": { "mutation": [ 459151 ], "stop": [ 459151 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EGP3", "query_cov": 1.0, "subject_cov": 0.5754475703324808, "identity": 0.987, "score": 440.0, "evalue": 1.22e-153, "gene_start": 1, "gene_end": 225, "reference_start": 1, "reference_end": 225, "reference_sequence": "MNTTKTLGLLFLGRLEKEKGFDLIYDFISQYPGKELPFDRYIFGSGSYESGILELSYHFKQIHFFGWKPLSEVERYLENIDYCLMPSRFLETFGLSAINILQQGIPIVGFKKGGLIPFIQDEYAIEQSEGSTDLAKFSNMLIKLQQEKKKKKPDFYEQLAQQSKDIANRYTVEKRYERFLSLTGTKKPQKIVLVSDFINKIGGIETYLHDTKHLLQQHGHQVKLFGGYCPKGPRGKLKKLLGIALSLVNLLAAIRFYFFLKREKPDLIRYHSMIRRNGWLLPRIARKFPATKRMMYHDFGYFTPYPHALTDTKQIKTPLSLKNYLAMTQTSNPLKKLFTFGKYLSLSLLVRRLKKTIDLHLVPSEFMEPIVQKSLKIAPNKITAFNHFLQK" }, "paralog": false, "identity": 0.9754601226993865, "score": 643.0, "evalue": 7.13e-236, "description": "Internal stop codon at 459151. Nonsense mutation around 459151.", "psc": { "uniref90_id": "UniRef90_A0A328EGP3", "product": "Glycosyl transferases group 1", "uniref50_id": "UniRef50_A0A2W4IJP5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EGP3", "UniRef:UniRef50_A0A2W4IJP5" ] }, "pscc": { "uniref50_id": "UniRef50_A0A2W4IJP5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4IJP5" ], "product": "Glycosyl transferase family 1 domain-containing protein" } }, "id": "NKOCPLAKPK_883", "locus": "GCA003260355_00847" }, { "type": "cds", "sequence": "contig_1", "start": 459292, "stop": 459651, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATCCGTCGGAACGGTTGGCTACTGCCTCGAATCGCTAGAAAATTTCCTGCAACGAAACGGATGATGTATCATGATTTTGGCTATTTTACGCCTTATCCTCATGCACTTACCGATACGAAACAGATCAAGACTCCTCTTACTCTAAAAAATTACCTTGCGATGACGCAGACTTCAAACCCACTCAAAAAACTCTTTACCTTTGGAAAATATCTTTCCCTTTTCCTCCTCGTAAGACGATTAAAGAAAACAATCGATCTCCACCTTGTTCCTTCTGAATTTATGGAACCGATCGTCCAAAAAAGCCTCAAGATCAGTCCAAATAAAATAACTGCATTCAATCATTTCCTTCAAAAATAG", "aa": "MIRRNGWLLPRIARKFPATKRMMYHDFGYFTPYPHALTDTKQIKTPLTLKNYLAMTQTSNPLKKLFTFGKYLSLFLLVRRLKKTIDLHLVPSEFMEPIVQKSLKISPNKITAFNHFLQK", "aa_hexdigest": "01280b55149b1dcb53cf379d773960e6", "start_type": "ATG", "rbs_motif": "TTATT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14084.709699999981, "isoelectric_point": 10.542337608337402 }, "id": "NKOCPLAKPK_884", "locus": "GCA003260355_00848" }, { "type": "cds", "sequence": "contig_1", "start": 459629, "stop": 459745, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAATGTCGATGCTGAGGGAATGCAAAAGTTTTTACTCACAAGAAATCAGGGGATCAGGTTTTTCTTTCTAGTGGGAAATGAAGAAATCACTATTTTTGAAGGAAATGATTGA", "aa": "MKNVDAEGMQKFLLTRNQGIRFFFLVGNEEITIFEGND", "aa_hexdigest": "230be8be966ee51cfac41c26b7f940d8", "start_type": "ATG", "rbs_motif": "TTTTT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4422.9904, "isoelectric_point": 4.7652902603149405 }, "id": "NKOCPLAKPK_885", "locus": "GCA003260355_00849" }, { "type": "cds", "sequence": "contig_1", "start": 459988, "stop": 460593, "strand": "-", "frame": 1, "gene": null, "product": "AB hydrolase-1 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBFBECE", "UniRef:UniRef100_A0A328EKM8", "UniRef:UniRef50_A0A328EKM8", "UniRef:UniRef90_A0A328EKM8" ], "nt": "ATGGAAAAGAAACAGCAAGTACTTCTAATCCATGGCGGGGATACTTACGAAAGTTATGCGGCATTTTTGCAAGCATTAAAGGGGAAGTCCGTGCACTTAGAGTGGATCGCGAGCAGGAGAGACTGGAAAAATGAGCTACAATCTCAGATCGGCGAAGGGTTTGTGGTTTATACCCCGCAAATGCCCAATAAACAAAACGCGAAATATGAAGAATGGGCGATTCTTTTCAAAAAACTATTGGAAGCGGTAGAAGACGGAGTTGTGCTAATCGGGCATTCCTTGGGTGCGGCCTTTTTGGTAAAGTATCTCTCAGAGCATCAAGTTGCCAAGCAAATCAAGAGTTTACATCTCTTGGGAACGCCCTTTGATGGGGATATGGAAAGTGATAAACTCGTAGGATTTGTCAGGACAGGGGAGTTATCGCAGCTGGAAGCACAAGTCAGAGAGATTTTCTTGTATCATAGCAAGGATGACTTCGCAGTACCGTATAGCCACTTTCTTAGGTATCAAGAGGCATTGCCATCAGCTCATTTTAGAGTTTTTGAGGATAGAAATCACTTTCTTCAGTCCGAAGTCCCAGAATTAAACGCTGATTTGATGAGGTAA", "aa": "MEKKQQVLLIHGGDTYESYAAFLQALKGKSVHLEWIASRRDWKNELQSQIGEGFVVYTPQMPNKQNAKYEEWAILFKKLLEAVEDGVVLIGHSLGAAFLVKYLSEHQVAKQIKSLHLLGTPFDGDMESDKLVGFVRTGELSQLEAQVREIFLYHSKDDFAVPYSHFLRYQEALPSAHFRVFEDRNHFLQSEVPELNADLMR", "aa_hexdigest": "d17b2182db524664af64922f55a38103", "start_type": "ATG", "rbs_motif": "TTTTTA", "ups": { "uniparc_id": "UPI000DBFBECE", "uniref100_id": "UniRef100_A0A328EKM8", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBFBECE", "UniRef:UniRef100_A0A328EKM8" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EKM8", "uniref90_id": "UniRef90_A0A328EKM8" }, "psc": { "uniref90_id": "UniRef90_A0A328EKM8", "product": "AB hydrolase-1 domain-containing protein", "uniref50_id": "UniRef50_A0A328EKM8" }, "pscc": { "uniref50_id": "UniRef50_A0A328EKM8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EKM8" ], "product": "AB hydrolase-1 domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_886", "locus": "GCA003260355_00850" }, { "type": "cds", "sequence": "contig_1", "start": 460983, "stop": 461357, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAATCAAGTCAATAAAAATCTCTAATCTCTTGAGTTTTTCCTATATGAATGAAAAAGAGTTTGAGAATCAGCCTCCTATTTCTTTTTATGGAGAAGATTTAGCCTATCAAGGAGTCAAAATCTTTATTGGAAATAATGCATCTGGGAAAAGTAATTTTATCAAGATTTTGGAAGAATTTTTTACGATTTTGATTAAAGATTTTTCGTTTGATAACAGCTTTTCTGATGCAGAGGAAATCCCTACGAGAAAGGCAATTAAAGAAAGAAATATTCTCACCAAATATCTCCAAACCAACAACCAAACACCAGATCAGCCTTCAAAACTAGAAATTGCTCTAGAATTGTCAAATACTGACTATGAAAACATCTGA", "aa": "MQIKSIKISNLLSFSYMNEKEFENQPPISFYGEDLAYQGVKIFIGNNASGKSNFIKILEEFFTILIKDFSFDNSFSDAEEIPTRKAIKERNILTKYLQTNNQTPDQPSKLEIALELSNTDYENI", "aa_hexdigest": "bec4a14c5b26ac19e469bf2bfaa5f9c9", "start_type": "ATG", "rbs_motif": "TTCTA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14316.969499999977, "isoelectric_point": 4.757105445861816 }, "id": "NKOCPLAKPK_887", "locus": "GCA003260355_00851" }, { "type": "cds", "sequence": "contig_1", "start": 461475, "stop": 462050, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAAGCATCATTTGATGAAAAGCTTCAAAAATTCATCATCAACGAATCTAAACTGAATGAATATCAGCTTTTTGTCCTTTCTTGTATTAGATACCAAAAGCTTTTGCAAATTGTTATTACCATTTTTAATCAATATGAGAAAAAACCTGAAGAAAGAAAGCGATATTTGCTCAAGAGAACGTTTGCAATTTTAAATAACGATAGGAGCCAGCTTAATTTCAAAAATTTTATCAATCCTCATGAAATCATCAACGATCTTGAATATTCAAGAAATAGTTTGGTCGGCTATACCGGCTGTATTTACAAGATTCGAAGCATTTTAGAAAATTTTACGCAGGAAGCACTCCTCTCTTCGGATATCAGCACCCAAGAAAAAGCGATAGAAGAACGTTTGAAAAAGTCAGACTTCTTTGTTAGTTTAAACTATATGATCCAAAAATACTTTGATAAACAGTTAAGAGTGGATTATGTGAATGGAATGATTGGATTTTATATGGTGGATGAGAATGATAGAGTTTCTTATTTTGATGACCTCAGTGATGGAGAGAAATCGCTCTTAACAATGATTTTTTGA", "aa": "MEASFDEKLQKFIINESKLNEYQLFVLSCIRYQKLLQIVITIFNQYEKKPEERKRYLLKRTFAILNNDRSQLNFKNFINPHEIINDLEYSRNSLVGYTGCIYKIRSILENFTQEALLSSDISTQEKAIEERLKKSDFFVSLNYMIQKYFDKQLRVDYVNGMIGFYMVDENDRVSYFDDLSDGEKSLLTMIF", "aa_hexdigest": "5d8ecbd13d17181749ac6984a12c8661", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 22856.922799999993, "isoelectric_point": 5.617249870300292 }, "id": "NKOCPLAKPK_888", "locus": "GCA003260355_00852" }, { "type": "cds", "sequence": "contig_1", "start": 462081, "stop": 462932, "strand": "+", "frame": 3, "gene": null, "product": "OLD protein-like TOPRIM domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4KG09" ], "nt": "ATGATTGTAAACGAACCGGAACTGCATCTTCACCCTCAATATCAGAAAGAACTCGCTGAAGTTTGCGACCAAATCAGCGAACATATTGGTGCACAGTTTATTCTCTCTACTAATTCTCCACTCTTCATCAATGAAAAAAATCTGACGAGCGTCTACAGGATGTATAAGAATAAAAATCAAAATTCTTTGGTTTCTGCGCCGAGAATCAATGTGGACTATGATGATGCGACACTGATGCATATGCTCAGATTTGAGAATCTTTCAAAGCTCTTCTTCGTTGATAAAATCATTCTTGTTGAGGGAGATACTGATGCTTACTTTTTCAGTTTTTATCTCAATTACCTAAAAACCTTCCCTGAGTGGAAGAATAAAATTCGTGATTATGAAATCATTAATATCAATGGAAAGGGGTCTTTTTCAACACGAAGAAAATTCCTCAGAAAGTTTAACATCAAGAACTATTTTATTGGAGACTGGGATAATACTGTTGATTATGGATTCTTTTCAAGGGCAGAACTCAATAGATTTTATATGCTCGCAAATAAACAGGCAAAAACTCAGAAATCTGAAAAAAGATATAGTGATTATTACAATAGACTCGTAAAAACTATCCTGACCTTCGCTCCAAAGAAGCATAAAGCGATCATAAAAGGGATTGAAAAACTTTATAACGACAAGGTTTTCATCCTCAAGGAAGGAGCAATAGAAACCTATGTTCCCCTCGAGAAAAAGGGGCTTTCTTATATGGCCTACTTCTGTAATGCCTACTTTTATGACCGAATCCTCAACCAAAATTTCAAAGAACAGAGAAAAGAATTAAATCAGATTATGAAAATTATTTTTAATGACTAA", "aa": "MIVNEPELHLHPQYQKELAEVCDQISEHIGAQFILSTNSPLFINEKNLTSVYRMYKNKNQNSLVSAPRINVDYDDATLMHMLRFENLSKLFFVDKIILVEGDTDAYFFSFYLNYLKTFPEWKNKIRDYEIININGKGSFSTRRKFLRKFNIKNYFIGDWDNTVDYGFFSRAELNRFYMLANKQAKTQKSEKRYSDYYNRLVKTILTFAPKKHKAIIKGIEKLYNDKVFILKEGAIETYVPLEKKGLSYMAYFCNAYFYDRILNQNFKEQRKELNQIMKIIFND", "aa_hexdigest": "60b4ec70060c81176f333bca730fd445", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A2W4KG09", "query_cov": 0.9929328621908127, "subject_cov": 0.9929328621908127, "identity": 0.858, "score": 488.0, "evalue": 2.51e-173, "valid": false, "product": "OLD protein-like TOPRIM domain-containing protein", "uniref50_id": "UniRef50_A0A2W4KG09" }, "pscc": { "uniref50_id": "UniRef50_A0A2W4KG09", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4KG09" ], "product": "OLD protein-like TOPRIM domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_889", "locus": "GCA003260355_00853" }, { "type": "cds", "sequence": "contig_1", "start": 463174, "stop": 463737, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EK41", "UniRef:UniRef90_A0A328EK41" ], "nt": "TTGGGCTTATTAGTTCCTATTCTTTTTTTCGTAGCATTAGGGGGATTGACCTTCCTTTTGTGTACCAAAGGATACTGCACGATTAGTACTATCGTTCCTATGCTTTCAGGATTAGGAGTGTTGTGGGTTAGTCTACGAGCTATAAGATTCATAACTCCATTTTTTATAGGAGAGGTCGCAAAAGCTGAAAATGCTAGTTACAGAACGATGAATGATATACAAAAACAAAGTATGAAAAATCTGACCAATTATATTATTTATGCGTTTTTACTTGGATTATTGCTCTTTATCTTACTTTTTATTGATCCTCGATCACTTGCCGAATCTTCAAAAGATTTCCTTGGTGAAATAGGGATTTCTCGAAGTATTCTTGAGGGAGAATACTTTGGAGATGTGATGGCGTGGCTGTTCTATGTACTTAATATTTCAGCGCTCATAGGAATTATCTCTACACTCAAAATGATTGCCAATGATTCCAGTAGAAGAGTAAATGATCGTTTGCAACAGCTCAGACTTCGTGCTTTTGAGGAAAGAAATACGAGGACTACGAGCTTTGGGCAGTAA", "aa": "MGLLVPILFFVALGGLTFLLCTKGYCTISTIVPMLSGLGVLWVSLRAIRFITPFFIGEVAKAENASYRTMNDIQKQSMKNLTNYIIYAFLLGLLLFILLFIDPRSLAESSKDFLGEIGISRSILEGEYFGDVMAWLFYVLNISALIGIISTLKMIANDSSRRVNDRLQQLRLRAFEERNTRTTSFGQ", "aa_hexdigest": "d1cb8810d81dba614b84f7c21ff69cf2", "start_type": "TTG", "rbs_motif": "TTTTT", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 463030, "stop": 463737, "observations": { "mutation": [ 463045, 463063, 463135, 463165 ], "stop": [ 463045, 463063, 463135, 463165 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EK41", "query_cov": 1.0, "subject_cov": 0.7957446808510639, "identity": 0.9790000000000001, "score": 344.0, "evalue": 1.29e-118, "gene_start": 1, "gene_end": 187, "reference_start": 49, "reference_end": 235, "reference_sequence": "MEILLGTGVILGIGTLILVSMCSSGQKEGSLLSRYSRKQGKSFFRGFFLGLLVPILFFVALGGLTFLLCTKGYCTISTIVPMLSGLGVLWVSLRAIRFITPFFIGEVAKAENASYRTMNDMQKQSMKNLTNYIIYAFLLGLLLFILLFINPRSLAESSKDFLGEIGISRSILEGEYFGDVVAWLFYVLNISALIGIISTLKMIANDSSRRVNDRLQQLRLRAFEERNTRTTSFGQ" }, "paralog": false, "identity": 0.9617021276595744, "score": 433.0, "evalue": 4.72e-156, "description": "Internal stop codon at 463045, 463063, 463135, 463165. Nonsense mutation around 463045, 463063, 463135, 463165.", "psc": { "uniref90_id": "UniRef90_A0A328EK41", "uniref50_id": "UniRef50_A0A328EK41", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EK41", "UniRef:UniRef50_A0A328EK41" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EK41", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EK41" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 21054.6168, "isoelectric_point": 9.226666831970213 }, "id": "NKOCPLAKPK_890", "locus": "GCA003260355_00854" }, { "type": "cds", "sequence": "contig_1", "start": 465345, "stop": 465665, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCCTATGCTCAGCTTGATCCTTTAGTAATTTATAAGAAAGAATCTTTTGAAAAGTTCCAGGAACTCCTCGCAAATATCACAAATAATACTGCATCTTATTTATTAAAATTAGACTTTGATGCAATTGCAGTTCAGCAGTCAGGACAACTTATTCAAGAAGAAAGCAATGAAGAAAAAGTAATTGAAATTCTGACTTCAGCAAGTAAAAATATCCCCCACCAACCAAGTAAACCTGAAACCGAAGTCCAAGATAGTAGAGCTCAGATATTTGATAACAGTAATGATACAGATGTTGAGGTATTTGAAGTGGAGGATTGA", "aa": "MSYAQLDPLVIYKKESFEKFQELLANITNNTASYLLKLDFDAIAVQQSGQLIQEESNEEKVIEILTSASKNIPHQPSKPETEVQDSRAQIFDNSNDTDVEVFEVED", "aa_hexdigest": "7ad6037eb1421f045c4ce6f6c338fef2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12053.13929999999, "isoelectric_point": 4.226001930236817 }, "id": "NKOCPLAKPK_891", "locus": "GCA003260355_00855" }, { "type": "cds", "sequence": "contig_1", "start": 465669, "stop": 466280, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGAAAGATGGGAGGCGAGAAAATTCAGTCTTTGGCTAGTATGCTCCTTTCCAAAACGGATCTTGAAAGCCTAGAACTCACCCAAAAACAATTTAGTAAATCAATTATCAGAGCTCAAAAGGAAATTGAAGGACGATATTTCGGCATCAGAAAGCATCTCTTTGATTATGATAGCGTAGTTGACAAGCAAAGAAAAAGAGTCTATCGTATCAGAGACGAAATCCTTGCTGCCGAAAACGATGAACAGAAGCAAGAAACTTATGTTGCTAACCTTAAAGAAGAGTTCCTAACTGAAGCTAAAAATATTATCATTACGCAAATTGCAAATGCTGAGATTACTGGACAGTCTGTTGCTGATCTTCTTGTAGTTCTGAATAAGGAATATGGCTTGAATATCACGCAACCAGAATACCATGAGCTGTCTCAGCTTGATTATGAGGCTTTGAAAGCGATGTTACTTGAGAGGTTAGACGCTTACTTTACTAGAGTGTTTTCTACACTTGAGACGAAGCTTCTATTCCAGATCTTTAGAGAAGTACATCTTCATTTTATTGATAAACTTTGGGTTGATCATATTGATGAAATGCAAAATCTTAGAGAAAAAGTCTGA", "aa": "MRKMGGEKIQSLASMLLSKTDLESLELTQKQFSKSIIRAQKEIEGRYFGIRKHLFDYDSVVDKQRKRVYRIRDEILAAENDEQKQETYVANLKEEFLTEAKNIIITQIANAEITGQSVADLLVVLNKEYGLNITQPEYHELSQLDYEALKAMLLERLDAYFTRVFSTLETKLLFQIFREVHLHFIDKLWVDHIDEMQNLREKV", "aa_hexdigest": "894f6f576c1445d30132bf2c1d7606da", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 23932.19360000002, "isoelectric_point": 5.645157814025879 }, "id": "NKOCPLAKPK_892", "locus": "GCA003260355_00856" }, { "type": "cds", "sequence": "contig_1", "start": 466404, "stop": 466517, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCAATTTAATAGGAAGAGAAAGGCAAATACCGTTCCTTATGCAAAAGTCAGCTTTATTCCTAGTCAAAAAGATACGAGCAAAGAAGCGATAGAAAAAGACCTTTTCTTCTGA", "aa": "MQFNRKRKANTVPYAKVSFIPSQKDTSKEAIEKDLFF", "aa_hexdigest": "b646d8cb497650bd5cc0f81e32053cc3", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4363.989899999999, "isoelectric_point": 9.871673011779784 }, "id": "NKOCPLAKPK_893", "locus": "GCA003260355_00857" }, { "type": "cds", "sequence": "contig_1", "start": 466674, "stop": 468014, "strand": "-", "frame": 2, "gene": "secA", "product": "Preprotein translocase subunit SecA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A6N9J083" ], "nt": "ATGAAGACCGGAGAAGGAAAAACGCTCGTTGCGGTTGCTCCTGTGTATCTTAATGCACTTGCTGGGAAAGGAGTGCATGTCGTAACGGTCAATGATTACCTTGCTTCTCGTGATAGTGAATGGATGGCGCATGTCTATACTTGGCTTGGATTGACCGTTGGTTCCGTGACCAAAGGGACTCCGCTTCAGATCAGAAGAGGAGAGTATGCTAAGGACATTACTTATGTGGAAAATTCAGAATTAGGATTTGATTACCTGAGAGATAATCTCGTAAAAAGTCTAGCAGAAAGAAGTCTGACTTGGAGACCACTTAATTTTGCGATTGTGGATGAGATTGATAGTATTTTGATTGATGAGGCGAGAACTCCACTTATTATCTCTCAGCCAAGAGAAGAACCTACTGAAAAATACAGCTATTATGCACAAGTAGTAAATCTGCTTTCTCCTTGTTCAGGAAAGAAAAAAGTTTCCAAAGGCTTTATTCAAGAGGTTTTGAATGATGAGTATGGGAAAGAGCAGGAAGAAGATGGGGATTATTACATTGATGAAAAGACCAAGACGGCTCAACTTTCTGGAAAAGGAATTGCAAAGCTTGAGGAGATTTTGAAGGTAGAGAATATCTATAAGGATATGGGGTATGGAGAAATCCACCATATTGAGAATGCTTTGAAAGCAAAGGCGGTCTATATCAAGGATAAGGATTACATTATCTCCAATGGGGAAGTGTTAATCGTAGATGAACATACAGGAAGGACGATGCCAGGAAGAAGGTTTTCTGAAGGACTTCATCAGGCGATTGAAGCAAAAGAACAGGTGCAAATCCAGAGGGAATCTCAAACAATGGCTACGATCACCTATCAGAATTTTTTCAAGCAATATGCGAAACTCTCTGGAATGACCGGAACAGCTTCTACTGAAGGAGAAGAGTTTAATAAAATTTATGAACTAGAAGTAGTTATTGTCCCTACGAATAAACCTATTCTTAGAGTAGATATGAATGATAAAGTATATTTCAATCAGCATACTAAGTGGAAGTTTGTGAAGGATTATATCAAATTTTATCATGAAATCGGACAGCCAATCCTTATCGGAACCGCGGATATTGCGACTTCTGAGTATGTTTCTAGGCTTTTGGATAAAGAAGCGATAACTCACTATGTTTTGAATGCAAAGTTTCACGAGCAGGAGGCGAATATTGTTTCTAATGCAGGGAAATATAAATCCGTAGTTGTGGCAACCAATATGGCAGGTCGTGGAACGGATATCAAGCTTGAAGAGGGATTGAACGAGAAGATGGCTCAGAATTATCTTAAATTTTTCCTAAAAAATCTAAAAAACTGA", "aa": "MKTGEGKTLVAVAPVYLNALAGKGVHVVTVNDYLASRDSEWMAHVYTWLGLTVGSVTKGTPLQIRRGEYAKDITYVENSELGFDYLRDNLVKSLAERSLTWRPLNFAIVDEIDSILIDEARTPLIISQPREEPTEKYSYYAQVVNLLSPCSGKKKVSKGFIQEVLNDEYGKEQEEDGDYYIDEKTKTAQLSGKGIAKLEEILKVENIYKDMGYGEIHHIENALKAKAVYIKDKDYIISNGEVLIVDEHTGRTMPGRRFSEGLHQAIEAKEQVQIQRESQTMATITYQNFFKQYAKLSGMTGTASTEGEEFNKIYELEVVIVPTNKPILRVDMNDKVYFNQHTKWKFVKDYIKFYHEIGQPILIGTADIATSEYVSRLLDKEAITHYVLNAKFHEQEANIVSNAGKYKSVVVATNMAGRGTDIKLEEGLNEKMAQNYLKFFLKNLKN", "aa_hexdigest": "db009ae04a2cebb45f7c5a1d318b2f6a", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0028DCEB4C", "query_cov": 0.952914798206278, "subject_cov": 0.8126195028680688, "identity": 0.536, "score": 425.0, "evalue": 5.47e-142, "valid": false, "gene": "secA", "product": "preprotein translocase subunit SecA", "ec_ids": [ "7.4.2.8" ], "uniref50_id": "UniRef50_A0A6N9J083", "kegg_orthology_id": "K03070", "go_ids": [ "GO:0005524", "GO:0006605", "GO:0006886", "GO:0017038" ] }, "pscc": { "uniref50_id": "UniRef50_A0A6N9J083", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A6N9J083" ], "product": "Preprotein translocase subunit SecA" }, "genes": [ "secA" ], "id": "NKOCPLAKPK_894", "locus": "GCA003260355_00858" }, { "type": "cds", "sequence": "contig_1", "start": 468048, "stop": 468179, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGACGATTTACTTCCAGAGGCGTTTGCCGTTGTAAAACAGGCTTGTAAAAGACTTGTTGGAACAAAAGCGAAAGTTAAAGGACATGAACTTACCTGGAATATGATTCCCTATGATGTTCAGTTGCTCTGA", "aa": "MDDLLPEAFAVVKQACKRLVGTKAKVKGHELTWNMIPYDVQLL", "aa_hexdigest": "a2a463b4f8ab63e0ddc89ddfae809ce6", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4870.755400000001, "isoelectric_point": 7.907514762878418 }, "id": "NKOCPLAKPK_895", "locus": "GCA003260355_00859" }, { "type": "cds", "sequence": "contig_1", "start": 468186, "stop": 468356, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAGATTGGATTATGAATATAATTGGTGGTGATTACAATCAAAAGCAGATTGATGCTTTGATGCCACTTGTGAGAGATATCAATCATCGGTATGCCGAATTTGATGCACTTTCTGATGCTGAAATTCAAGCAAAAACCGAAGAATTTAAACAAAGAATTGAAAACTGA", "aa": "MLDWIMNIIGGDYNQKQIDALMPLVRDINHRYAEFDALSDAEIQAKTEEFKQRIEN", "aa_hexdigest": "f2e885287f08fe47c9224a2cb36aa1ed", "start_type": "ATG", "rbs_motif": "TTTA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6612.395199999999, "isoelectric_point": 4.498829078674317 }, "id": "NKOCPLAKPK_896", "locus": "GCA003260355_00860" }, { "type": "cds", "sequence": "contig_1", "start": 468721, "stop": 468921, "strand": "+", "frame": 1, "gene": null, "product": "CsbD-like domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4IGJ7", "UniRef:UniRef90_A0A328EGW7" ], "nt": "ATGACGTTGATTGGAATCGTTTTGTTGATTTTGGCGCTGATTCAGCTAAGAGAGTTCCTTTGGGGAATTATTCTTCTTTTGGCAGGTCTACTTTGTGTTATGGGATATTTTAATCCTTTTTTGAGAAGTTTGATTGATAGATTTTTTTCAGAATCAGAGGACACTTCTAAAAAAGACACCCATTCAAAGAAAGAAGACTAA", "aa": "MTLIGIVLLILALIQLREFLWGIILLLAGLLCVMGYFNPFLRSLIDRFFSESEDTSKKDTHSKKED", "aa_hexdigest": "745f50c30325cbd74d6d0618f2aadb44", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 468550, "stop": 468921, "observations": { "mutation": [ 468562 ], "stop": [ 468562 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EGW7", "query_cov": 1.0, "subject_cov": 0.5365853658536586, "identity": 1.0, "score": 118.0, "evalue": 5.74e-33, "gene_start": 1, "gene_end": 66, "reference_start": 58, "reference_end": 123, "reference_sequence": "MQKKGKTAGERIDEATGKIQEAASKFGEKIGNGAEETKKVANNVKNWREKASVEEIVMTLIGIVLLILALIQLREFLWGIILLLAGLLCVMGYFNPFLRSLIDRFFSESEDTSKKDTHSKKED" }, "paralog": false, "identity": 0.991869918699187, "score": 238.0, "evalue": 9.4e-83, "description": "Internal stop codon at 468562. Nonsense mutation around 468562.", "psc": { "uniref90_id": "UniRef90_A0A328EGW7", "product": "CsbD-like domain-containing protein", "uniref50_id": "UniRef50_A0A2W4IGJ7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EGW7", "UniRef:UniRef50_A0A2W4IGJ7" ] }, "pscc": { "uniref50_id": "UniRef50_A0A2W4IGJ7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4IGJ7" ] } }, "id": "NKOCPLAKPK_897", "locus": "GCA003260355_00861" }, { "type": "cds", "sequence": "contig_1", "start": 469621, "stop": 469950, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAATAGATTTGTCTACCATTTTCACTATATGGAATATACCTCTGAGGAGAAAATCTTGATCATCCAGCAATATTTGGCAAAATATCAAATCCCATACAATGCAGATTTATTACCCACTTTTGCACGCAAAGTCTCACCTGTTCCTAGAGAGATTCACAATCTTGTAATTAAAATGAGAGATTTTGTTGTTAGTAAAAAGATACAAAATCTGGACAAAAAAGAGCGATGCGATTTTCTCGACCATCTTCAGATTGACGATGGTGGAATGATGCCCCTCCACAAAAAATACTTGGAAATTCTTTCTCACTTTGATCGCCCCGTCTGA", "aa": "MKNRFVYHFHYMEYTSEEKILIIQQYLAKYQIPYNADLLPTFARKVSPVPREIHNLVIKMRDFVVSKKIQNLDKKERCDFLDHLQIDDGGMMPLHKKYLEILSHFDRPV", "aa_hexdigest": "238ef6d1d76f56a0bed6342489f47cb8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13182.291799999988, "isoelectric_point": 8.967955207824708 }, "id": "NKOCPLAKPK_898", "locus": "GCA003260355_00862" }, { "type": "cds", "sequence": "contig_1", "start": 470506, "stop": 470790, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGGGCATTTTTAGGCAAAGAAGAGGTAATACAAATTTTTGGGAAAGATCTTTTTGATGCAGAGGAGTTAGTAGAACTTTTTTCTGAACTCATGGGGATATCAGGCATAAAACCTTTTGAATGCGTGGGGACAAACGAAGAAATGCTTTTAGGACTTCGGCTTTCATATCTCAAAAACCCGGAAGAAAATTCAAAGATTATGCTCCTTTTCAGGGAGAAGATTATGACAAAAATGGAGGAGTCAGATTTTCTTGCTTTACAAAAAAAATTACTTGCCAATTAA", "aa": "MRAFLGKEEVIQIFGKDLFDAEELVELFSELMGISGIKPFECVGTNEEMLLGLRLSYLKNPEENSKIMLLFREKIMTKMEESDFLALQKKLLAN", "aa_hexdigest": "1f1de5c01100fa32d2d38a40f7d165b3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10834.663099999989, "isoelectric_point": 4.777737998962402 }, "id": "NKOCPLAKPK_899", "locus": "GCA003260355_00863" }, { "type": "cds", "sequence": "contig_1", "start": 471466, "stop": 471819, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAACCATTTTCTACTTTCAATATTTCCCGTTTTGAGTGGAATCCAAATACCTATGAGGCAAAATTCCATTACTCATTTGATGAGAGCGAATCCTTTACAGAAGTGATCAATTTTTCTCCTTTAAAAGAGAAATCTGAATTCCCGATAGTAAAAAATGATGAAAACGAACTCAAACAACTTCTTGCTCATCTCCATCTCGCATTAGGGGTCAGTTACTACAAACTCTTTCCAACAAAAGAGATTCTTGTCCATACGCTCGATTTAACGCAAGAGCAAAAAAGATTTTGGAATAATTTTTATATTAAAGGACTCGGAGAATTTTTTTATAGAAACCAGATAAATCCTGAATGA", "aa": "MKPFSTFNISRFEWNPNTYEAKFHYSFDESESFTEVINFSPLKEKSEFPIVKNDENELKQLLAHLHLALGVSYYKLFPTKEILVHTLDLTQEQKRFWNNFYIKGLGEFFYRNQINPE", "aa_hexdigest": "73d7a9d5fc701b6ea36b76148334ff6d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14093.735399999983, "isoelectric_point": 5.809990882873536 }, "id": "NKOCPLAKPK_900", "locus": "GCA003260355_00864" }, { "type": "cds", "sequence": "contig_1", "start": 472275, "stop": 472541, "strand": "-", "frame": 2, "gene": null, "product": "Endonuclease/exonuclease/phosphatase domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A948C4X6" ], "nt": "ATGGGAAATAACAATATAGATGCAATCAAAAAAATGATTTTAGATGAAAACCCAGATGTTGTTCTTTTTGTCGAGTTTAGTGAAGAGCATAGTCAAGCTTTGAAGGATTTTTTTGCGGCACATTATCCCTATAGCAATACCACCTCTCGATCCAAAATCGCGGTTGGAAGCATCGTATTTAGTAAATATCCAATAGATAATCTTGCTGACGACTTCCCTCAGGCAAGTTGGAGATATGGCTATTTCTCTATCGATACAGAAAAATGA", "aa": "MGNNNIDAIKKMILDENPDVVLFVEFSEEHSQALKDFFAAHYPYSNTTSRSKIAVGSIVFSKYPIDNLADDFPQASWRYGYFSIDTEK", "aa_hexdigest": "782463cfa282571ca8b0a91109098296", "start_type": "ATG", "rbs_motif": "TTATT", "psc": { "uniref90_id": "UniRef90_A0A948C4X6", "query_cov": 0.8181818181818182, "subject_cov": 0.8888888888888888, "identity": 0.597, "score": 89.7, "evalue": 7.83e-22, "valid": false, "product": "Endonuclease/exonuclease/phosphatase domain-containing protein", "uniref50_id": "UniRef50_A0A948C4X6" }, "pscc": { "uniref50_id": "UniRef50_A0A948C4X6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A948C4X6" ], "product": "Endonuclease/exonuclease/phosphatase domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_901", "locus": "GCA003260355_00865" }, { "type": "cds", "sequence": "contig_1", "start": 472776, "stop": 473000, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTGGCAAGAAACTGATTGCCAATTCTCCGAAATAACTCCCTTTACAGAGGATCACAACTCCTCCAAAAAGTATTCGCAAGGAAGCAAATATAAGACTCATATTGCTCTATTTACTAGGGATAAACGGTTTTTGACTATATATATTCTTTATGAGAAAGCAAATGTTTTTGTTTTTATCAAAAAGTTGGGGAATAAAACTAAGGAAAATCTTGCTTTTAGCTAA", "aa": "MWQETDCQFSEITPFTEDHNSSKKYSQGSKYKTHIALFTRDKRFLTIYILYEKANVFVFIKKLGNKTKENLAFS", "aa_hexdigest": "b3a73f9c7319c16734e2e29d6425d893", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8805.957299999991, "isoelectric_point": 9.301450157165526 }, "id": "NKOCPLAKPK_902", "locus": "GCA003260355_00866" }, { "type": "cds", "sequence": "contig_1", "start": 473087, "stop": 473371, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGGACTTTTCGGTTTTGTTTTTAGCGTGAATATCCTTTTTGCTAGCTCTGGTAGAAACTCTGTTTTCTATCAGAATTTAAATCAATTATTAGTTCCAGTAGAGGCTATTCTTCGCCAAAAAACACTTTCTCGTTATCAGCTTACGAGGCTCTTGAATGCAGTAGAATGCTATGATTGTATAGTCCCGGATAAGAAAACTCATGACAAATATAGTGGAGCCTTTTGGACGGGGTTTGTTTCGTTACCGGGTAAAGATTTTAGGGATATTGAGTATCTTTCCTGA", "aa": "MGLFGFVFSVNILFASSGRNSVFYQNLNQLLVPVEAILRQKTLSRYQLTRLLNAVECYDCIVPDKKTHDKYSGAFWTGFVSLPGKDFRDIEYLS", "aa_hexdigest": "270b080bc16fca66881507b43e532448", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10762.27009999999, "isoelectric_point": 8.638328361511231 }, "id": "NKOCPLAKPK_903", "locus": "GCA003260355_00867" }, { "type": "cds", "sequence": "contig_1", "start": 473402, "stop": 474091, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02412.23" ], "nt": "GTGGCAAAGGTTGGTAACGATGATACCATGAGAGGATACCCAGTTGCAACCAGTAATATTTGTGCTGGAAAGTTTTGTGGATCAAGAGATATCAGTAAGGCAGAATTTTTTCAAACCCTTCTCAATCTTCTCACTGATAGGATAAAAGGCAACTACTCTGCCCCATGGAAAGACATTAAAGAATGGGTTAATAAAAGAGACAAAAAAAGCTATGAATATAAAGTATTTACTACTGCAGAGATTGACCTAATAAATAGAAAAGAAAAACAGACTGAAAAAATACAGTCGAGACTTGAATTTTCTACATATCTTAAATACTGTATGTTTCATCCGAACGAATGTGGATTTCAGACTTTCCCTACTTTACCAAAAGGATATTGGCCTCTTTCAGAGATGAATATTCTGATTCAGGCGGGAATTATTACTCCTCAGGATGTAGAAGGCTTTTCTAAACCTATTAGTACGGAAGATGCATTAAAAAAGCTGGATCTCCTTTATGAACTTCATATTCAATGTGCATTAAATGTTGACTATGACTGTGATGGACTAGTAAATCATGAAGATAATTGTCCTTATGCTTATAATCCTGGACAAAATGATATGGATAGCAATGGTATTGGCGATGTTTGTAGTGATGATATTGATGGTGATGGAAAAAAGAACCCGCTTGGTCTGGTTGATGATACCTGA", "aa": "MAKVGNDDTMRGYPVATSNICAGKFCGSRDISKAEFFQTLLNLLTDRIKGNYSAPWKDIKEWVNKRDKKSYEYKVFTTAEIDLINRKEKQTEKIQSRLEFSTYLKYCMFHPNECGFQTFPTLPKGYWPLSEMNILIQAGIITPQDVEGFSKPISTEDALKKLDLLYELHIQCALNVDYDCDGLVNHEDNCPYAYNPGQNDMDSNGIGDVCSDDIDGDGKKNPLGLVDDT", "aa_hexdigest": "6ed0c1b37caf6150aee1510b06b19112", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02412.23", "name": "TSP_3", "length": 22, "aa_cov": 0.09606986899563319, "hmm_cov": 1.0, "evalue": 8.173640929497286e-11, "score": 37.92822265625, "start": 200, "stop": 221 } ], "seq_stats": { "molecular_weight": 25941.9632, "isoelectric_point": 4.825653266906738 }, "id": "NKOCPLAKPK_904", "locus": "GCA003260355_00868" }, { "type": "cds", "sequence": "contig_1", "start": 474113, "stop": 474271, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGAAAAATCTGAAGATCCGACTCCATTAGGAGAAGATACAAGAGAAGACCTTTATTTCATCAAGGTAAGCAAACTTGATAATGATCTGCCTTCTTTGGTAAAATTTGAGATTACGAGTCGTGAGGCTCCATTAGCAGTTGAATGGGATTTTTGA", "aa": "MKEKSEDPTPLGEDTREDLYFIKVSKLDNDLPSLVKFEITSREAPLAVEWDF", "aa_hexdigest": "5b49562881cba54230434d54f687f763", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6043.716299999999, "isoelectric_point": 4.35531063079834 }, "id": "NKOCPLAKPK_905", "locus": "GCA003260355_00869" }, { "type": "cds", "sequence": "contig_1", "start": 474329, "stop": 474424, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACTGTTATTGCAAAGGTTACTACCAAAGGAGGGAAAGTCTTTGTCCTTAGTCAGCAACTTTCGCTCGGACAACCGCAGAGCACGATGTATTGA", "aa": "MTVIAKVTTKGGKVFVLSQQLSLGQPQSTMY", "aa_hexdigest": "24ebd7d5805252fc0fd93e7401198874", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3341.9369, "isoelectric_point": 10.00041637420654 }, "id": "NKOCPLAKPK_906", "locus": "GCA003260355_00870" }, { "type": "cds", "sequence": "contig_1", "start": 474644, "stop": 474883, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATTTTCTGGATCAGAACTTTATTTCACGGATCGGTATAGGTATGTGTAGCTACGAGTTTCCTATCAATAAAATTCGTCCCATCGCCAAAATCCCATTTAACAGAATCTATAGCACCAAGACTAAGATTCTGAAGTCTGAGTGTCGTCATTATTTTGGTCGTAGTAGTCTTGAGATGCGGCGAATACTCAGGACTAAATCCAAAGAAAGTTCAGTTTTGATCAAGAATATCTATTGA", "aa": "MNFLDQNFISRIGIGMCSYEFPINKIRPIAKIPFNRIYSTKTKILKSECRHYFGRSSLEMRRILRTKSKESSVLIKNIY", "aa_hexdigest": "c8348e294f40900fa150d01d558b484a", "start_type": "ATG", "rbs_motif": "TTCTT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9392.03479999999, "isoelectric_point": 10.29194240570068 }, "id": "NKOCPLAKPK_907", "locus": "GCA003260355_00871" }, { "type": "cds", "sequence": "contig_1", "start": 475547, "stop": 475822, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATTGATAAGGGGGAAACCTGTAAAACTTGTCCTCAAGACGTAGGTCCATGTACTGCTTTTTGTGATAATGGTAAGATTGAGGCGGCCGAAACCTGTAAAAACTGTGAAAAAGATGTAAAAATCTGTCTCGTAAGCTGTGGGAATGGGAAAATTGAGGAAGGTGAAATTTGTGATGATGGAGATCAGAATTTTCATAATGGTAAATGTTCTGTGGACTGTAAACCTATGGACCCTAAGAAACCCCTCTGCGGAAACGGAGAAATCGACCTATGA", "aa": "MIDKGETCKTCPQDVGPCTAFCDNGKIEAAETCKNCEKDVKICLVSCGNGKIEEGEICDDGDQNFHNGKCSVDCKPMDPKKPLCGNGEIDL", "aa_hexdigest": "92bc864fdaa296b744fa03bc14f25e70", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9746.999599999992, "isoelectric_point": 4.549472618103027 }, "id": "NKOCPLAKPK_908", "locus": "GCA003260355_00872" }, { "type": "cds", "sequence": "contig_1", "start": 476278, "stop": 476547, "strand": "-", "frame": 1, "gene": null, "product": "DUF721 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4IJE2", "UniRef:UniRef90_A0A660MXX1" ], "nt": "ATGAAAGCATTTCAAGACCTCTCTCGTAAGCTTTTTTCTGAGAAATTTCAGGGGAATCGTATGCTAGGCACTCTTGCACTTAAGGTTGTTAAGGACCATTTTCATATCAAAAATACAAATAAGAATATCGTCACAGACGAGCTCATTGAAGGCTATTTGAAATACCAAACACTTTTCCTCAAAACTTCAGATCAAGCTATGAAAATAGCAATTTTTAAAGAAAAAGAAAAACTGATTGAGGCAATCAATCAGCATTTCAAAAAGTTGTGA", "aa": "MKAFQDLSRKLFSEKFQGNRMLGTLALKVVKDHFHIKNTNKNIVTDELIEGYLKYQTLFLKTSDQAMKIAIFKEKEKLIEAINQHFKKL", "aa_hexdigest": "8dee2ba0202dfe305227b2f48e5466a9", "start_type": "ATG", "rbs_motif": "TTATT", "psc": { "uniref90_id": "UniRef90_A0A660MXX1", "query_cov": 1.0, "subject_cov": 0.8725490196078431, "identity": 1.0, "score": 173.0, "evalue": 8.87e-55, "valid": true, "product": "DUF721 domain-containing protein", "uniref50_id": "UniRef50_A0A2W4IJE2" }, "pscc": { "uniref50_id": "UniRef50_A0A2W4IJE2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4IJE2" ], "product": "DUF721 domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_909", "locus": "GCA003260355_00873" }, { "type": "cds", "sequence": "contig_1", "start": 476920, "stop": 477276, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MWL0", "UniRef:UniRef90_A0A660MWL0" ], "nt": "ATGGAACTCAATTTGCTAGAGTATGATATTGACTCTATAAAAAGCATTTTACAAGGCATTCTCGATAAACACCTTCCAGAAGGACAGCAAATTTCGGTTCAAGAGCTTGATGAACAGGGAATCGTATGGCTTATACAACTTCTTTTGGAGAAAGATGATAAAAAGAGAGAACAGCTCTGGCAAGAGATTGAAGATAAATATCAGGTTGCTTCTAATGATTTTAGAAATACCTATCAAAAAATCCTTGAAATTTGTGATCAAATTAAAATCACCGAACAACTTGGAACTTCACTTGTAGAACTCAAATCAAATATCAAAAATGATCATTCACTTTCACAACTAGAACAAGAAATCTGA", "aa": "MELNLLEYDIDSIKSILQGILDKHLPEGQQISVQELDEQGIVWLIQLLLEKDDKKREQLWQEIEDKYQVASNDFRNTYQKILEICDQIKITEQLGTSLVELKSNIKNDHSLSQLEQEI", "aa_hexdigest": "457c1903653b519f3fe671327a4ba73f", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A660MWL0", "query_cov": 1.0, "subject_cov": 0.9833333333333333, "identity": 0.983, "score": 223.0, "evalue": 1.56e-73, "valid": true, "uniref50_id": "UniRef50_A0A660MWL0" }, "pscc": { "uniref50_id": "UniRef50_A0A660MWL0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MWL0" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13866.527799999985, "isoelectric_point": 4.442331123352051 }, "id": "NKOCPLAKPK_910", "locus": "GCA003260355_00874" }, { "type": "cds", "sequence": "contig_1", "start": 477303, "stop": 477542, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACCGAATATAAAAATCAATCAGTAGACCAAATCCAGAAGCAGCTTTCAAATAAGTTTGAAAAAGACAAGCAGTGGCGAATTAGAGATGATCAAGAAATCTATCTTGCTGGACAAGCAATTATTAATGAAACCATAGGCAAGATCCAGGAGAGTGGGAATAACTGGGGGAAATGGGCTTTAGAACCTTTGAAAACTTTGGAAAAGGAACTTGTAAAAAAGCATAACGAATGTCAATGA", "aa": "MTEYKNQSVDQIQKQLSNKFEKDKQWRIRDDQEIYLAGQAIINETIGKIQESGNNWGKWALEPLKTLEKELVKKHNECQ", "aa_hexdigest": "574ba369be8e1e12363f576e0b6f4041", "start_type": "ATG", "rbs_motif": "TTTA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9376.472599999996, "isoelectric_point": 6.5756120681762695 }, "id": "NKOCPLAKPK_911", "locus": "GCA003260355_00875" }, { "type": "cds", "sequence": "contig_1", "start": 477630, "stop": 477914, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCGTAATAATGCCCATAGAGATATTATCCAAAAAAATACTGAAACAAAAGATACTCAAGGTAAAGCATTTTTTAACTTCGACAAAAAAACAGACACCTATATTTTTACCGATAGAAGTAACAAACCGAAGATTCACGAAGTTTTAGGTCAGTTTTTTACTAATGATAAAAATATCTATACTATTGATTACTCAAAATGTACTAATTTGAAAATCAAAAATAAGATGCAACAAGTATTGGGAGGTACAAGTAGTTGCCTGATCAAATATGATAAAAAAACATGA", "aa": "MRNNAHRDIIQKNTETKDTQGKAFFNFDKKTDTYIFTDRSNKPKIHEVLGQFFTNDKNIYTIDYSKCTNLKIKNKMQQVLGGTSSCLIKYDKKT", "aa_hexdigest": "5e4d0813498d10f9528dc25689eaf0b8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11018.42699999999, "isoelectric_point": 9.598198127746581 }, "id": "NKOCPLAKPK_912", "locus": "GCA003260355_00876" }, { "type": "cds", "sequence": "contig_1", "start": 478269, "stop": 478382, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAGGGGCATTTTATCAATCGTCAAGGGACAAAAAAGGATATAAAAATTTGGGAAGATAAAGATTCATTTAAAAATAATGCTCTTTATGATATCTTGGACAACAATGAATGA", "aa": "MEGHFINRQGTKKDIKIWEDKDSFKNNALYDILDNNE", "aa_hexdigest": "14b2c13ea4ec078e7279d720139c5fb3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4440.857000000001, "isoelectric_point": 5.153557395935057 }, "id": "NKOCPLAKPK_913", "locus": "GCA003260355_00877" }, { "type": "cds", "sequence": "contig_1", "start": 478734, "stop": 478841, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAGATAGAACTGCCAGAGAAACTGTTGCAAACCTTCTTAGTGGAGATAAGACGAACGCACAGAGAAGTATCAGAGAATTAGGGAAACATCTCAATGTATGGTGA", "aa": "MTDRTARETVANLLSGDKTNAQRSIRELGKHLNVW", "aa_hexdigest": "a7fce8ea9dbcf71a7c667cb51282f33b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3982.444200000001, "isoelectric_point": 9.968955802917481 }, "id": "NKOCPLAKPK_914", "locus": "GCA003260355_00878" }, { "type": "cds", "sequence": "contig_1", "start": 479046, "stop": 479375, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAAAATAATGGGTTGATACCAGCAAATGCACCATTTGATGAAGAAATGAAAAAAGGATGCCAAAAAATCTATGACAGCTTAAGAGCTAAGAAACAAGCTATTGAACAACTAGATTATAGTCCTACTGCATTCCAAAGCAAAATGGAAAATGAAAAGATCAAACTAGAAGCCAAAGGGAATAATCTGACTGATGATGATAGGATCAGACTTCAAAGTATTTCAGTAATGCTTGAGAATCCTCAAATGCTCCAGGAAGCACTCCAAAATGCAAAATCAGCAGCTGAAAATAGTCTCAAATATGAAGGAATTGGCGCTCTTCTCAAGTGA", "aa": "MQNNGLIPANAPFDEEMKKGCQKIYDSLRAKKQAIEQLDYSPTAFQSKMENEKIKLEAKGNNLTDDDRIRLQSISVMLENPQMLQEALQNAKSAAENSLKYEGIGALLK", "aa_hexdigest": "6628d46e5637fab975e154790f54eeb5", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12245.82919999999, "isoelectric_point": 5.501582527160643 }, "id": "NKOCPLAKPK_915", "locus": "GCA003260355_00879" }, { "type": "cds", "sequence": "contig_1", "start": 479511, "stop": 479717, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATCTTGGAAGAGGTTGCAATCTGTATCGTTGCTATCGCACTTGGAGCAGTGACAGGAGGCGTCGGGACTGCAGCTATTTATGGTGCTAGATTCGCTCTTAAGGCAGCACAGGGTGCAAGATATGCAAATAAAGCCTATAAGATCAAAAAAACATTTACGCTGATGAGAAAACTCTCTAAAGCAAGGAAATATTGGAAAATGTGA", "aa": "MILEEVAICIVAIALGAVTGGVGTAAIYGARFALKAAQGARYANKAYKIKKTFTLMRKLSKARKYWKM", "aa_hexdigest": "de5caa43f57387e85b226b68d1cfca9e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7396.898700000004, "isoelectric_point": 10.345451164245603 }, "id": "NKOCPLAKPK_916", "locus": "GCA003260355_00880" }, { "type": "cds", "sequence": "contig_1", "start": 479823, "stop": 479921, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATATGCAAATAAACTCAATGATGCAAGAAGATCTCTAGATGCAATCAATAAAAGCAATAAATTTGTGCACCTTAATGACCTTGCAAAAGTTTGA", "aa": "MKYANKLNDARRSLDAINKSNKFVHLNDLAKV", "aa_hexdigest": "c76351f3edee20900e527c9cff758732", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3688.2219000000005, "isoelectric_point": 9.99467868804932 }, "id": "NKOCPLAKPK_917", "locus": "GCA003260355_00881" }, { "type": "cds", "sequence": "contig_1", "start": 479982, "stop": 480230, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAGTAGTACTGTTCTTAGAAATGCACTCCATGGGAAAAACCTTACAGATGGGGTCAATCCTTTTGGCTATACTGAAGGACCTAATGGTGAACAAATTTCTAATTTTAGGGGATATGTCCAAAGTATCGCATTTTTAGGGATTTTGAAAGCTATCGGACAACCGCTCCAAAATCTTACTGGAGCTGCATTACAGAAGCTTATGGGAGAAAAAATTGCAGCGAACTGGCTCGGAAAAGTTTTACAATGA", "aa": "MSSTVLRNALHGKNLTDGVNPFGYTEGPNGEQISNFRGYVQSIAFLGILKAIGQPLQNLTGAALQKLMGEKIAANWLGKVLQ", "aa_hexdigest": "9999686612a76b5555d9c00d3ce71f00", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8771.0056, "isoelectric_point": 9.600454521179199 }, "id": "NKOCPLAKPK_918", "locus": "GCA003260355_00882" }, { "type": "cds", "sequence": "contig_1", "start": 480588, "stop": 480806, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGAAAAAGAGAACAACTCAATCAAGAAATAACTAAGCTAAAAAATCAAGGAATAGCTTCCACAGACTCTAAAATCACCACACGTGAAAATGCAGTAAAATTCATAGAAAATAAACTCAATTCAGCAAGTTATAAGGAAATTTCCTTGCCAGAATCTAATAAAGTTTCTATCAAAAATGCATCTTATAATATGGCTGTTAATAGGTATGCTAATTGA", "aa": "MRKREQLNQEITKLKNQGIASTDSKITTRENAVKFIENKLNSASYKEISLPESNKVSIKNASYNMAVNRYAN", "aa_hexdigest": "59599a9f5d5ad604f558faa3fa5997cc", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8221.216099999998, "isoelectric_point": 9.849882698059083 }, "id": "NKOCPLAKPK_919", "locus": "GCA003260355_00883" }, { "type": "cds", "sequence": "contig_1", "start": 481182, "stop": 481445, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCGTGATGCAAATAATACTATGATTCAAGAATTATCTAGAAAGATTGATAGCATAGAGGCTGAACTCAAAGCCAAAAAAGAAAAATCAGTAGAAGAAAAAGCAGATGAGATTTCAAAGAACAGACCAGAATATTATGAAAAATATGCAAAAGAAAGTCATACGGACCTATACGAACAATATAAAACCTATCAAAATACCCCAAATGAACTCAAACGTTTGGATACTGAATTTGCTGAAAGATTAATTTTGGACAATCCTTGA", "aa": "MRDANNTMIQELSRKIDSIEAELKAKKEKSVEEKADEISKNRPEYYEKYAKESHTDLYEQYKTYQNTPNELKRLDTEFAERLILDNP", "aa_hexdigest": "0598c34755b32387f7766fc1d54741ba", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10417.448199999988, "isoelectric_point": 5.0920007705688475 }, "id": "NKOCPLAKPK_920", "locus": "GCA003260355_00884" }, { "type": "cds", "sequence": "contig_1", "start": 481761, "stop": 481910, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGAATCAATTCGTTCATAGACTTCCTTGCTGTTGGTAGGATTAAAATCTTTAAAAAGATTAGATTCTATGACCTCTCTCGGGATATTAAGCAGTTTTTTTGTTTTTTGTTGTTGTATGTCATTTCATTGGTTGTGCACTCCCGTTGA", "aa": "MRINSFIDFLAVGRIKIFKKIRFYDLSRDIKQFFCFLLLYVISLVVHSR", "aa_hexdigest": "545847de8e81c5727950b249705b7f83", "start_type": "ATG", "rbs_motif": "TTTTT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5971.179399999998, "isoelectric_point": 10.286398124694824 }, "id": "NKOCPLAKPK_921", "locus": "GCA003260355_00885" }, { "type": "cds", "sequence": "contig_1", "start": 482253, "stop": 482525, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAGAAATAAAACGATTCCTGCACGAGAGCAACAAGCGTATCATGAACTACAAACTATACATAAAGAATACCTCAGTCATGCAAAAAATCATCCATATAATGATATTATTCGTAACACATCTGGAGAAGAAAATCATGTAAATAAACGAGAAGAATTTTGTACAGAAGCTGTAACTGAACCCCTCTTTATGCAATATCTTTCAGAGATCCCATACAAAACGCAAAAGAGTAGATACGAGTTTCTTATGAAAGAAAATCTAAAAGAAACATGA", "aa": "MRNKTIPAREQQAYHELQTIHKEYLSHAKNHPYNDIIRNTSGEENHVNKREEFCTEAVTEPLFMQYLSEIPYKTQKSRYEFLMKENLKET", "aa_hexdigest": "38c167bcc6b294495a039d7a0a9f30cb", "start_type": "TTG", "rbs_motif": "TTTA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10889.099599999994, "isoelectric_point": 6.511440849304199 }, "id": "NKOCPLAKPK_922", "locus": "GCA003260355_00886" }, { "type": "cds", "sequence": "contig_1", "start": 482547, "stop": 482672, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTTATAAGATGCTTGACCTTTACTACACCAATATCAACAATACAAGAAGAGAATATTTTAGTTGGAATAAAAATAGAACTCTAGAAGCTAGAGAACTTCAAGAAAAACAACAATCTCAATGA", "aa": "MLYKMLDLYYTNINNTRREYFSWNKNRTLEARELQEKQQSQ", "aa_hexdigest": "388610c2714b6cfcc5c2040d1571539f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5217.806500000001, "isoelectric_point": 9.221573829650879 }, "id": "NKOCPLAKPK_923", "locus": "GCA003260355_00887" }, { "type": "cds", "sequence": "contig_1", "start": 482901, "stop": 483101, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGGATATACTACTACAGGAGAAGTTAGATTATTTTATAGATCTATATCAGAAGGGATATGGAGAAGTACTGATGGATCAGACGGACCTATTATTAGTAAAGCCCAATATTTACCTAATGGTTCATATGAGACAACCACTCAAGTTGATTTTCGCTTAGGAAAAATTTTCGATAAGATTCCTATGCAAACAATGCCATGA", "aa": "MGYTTTGEVRLFYRSISEGIWRSTDGSDGPIISKAQYLPNGSYETTTQVDFRLGKIFDKIPMQTMP", "aa_hexdigest": "fdcd9b177f74c8f4c01e1567d757db2d", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7454.342100000001, "isoelectric_point": 6.080999183654785 }, "id": "NKOCPLAKPK_924", "locus": "GCA003260355_00888" }, { "type": "cds", "sequence": "contig_1", "start": 483252, "stop": 483455, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTGAAGTTAAAAAAGGGTATGAAAATTATAATATAAAACTACAATTAGAAAAAATTATAGATAGCTACACATTTTCTCATAAGATTCTAGGAGATATCCATGTGGATATTGTGAGAGGGAACATACAAGGGCAAGATGTAAATATTCATTTTGGTCATGCAGTTAATAATGATCCGAATAGTGTCTTTATTATTAGGTGA", "aa": "MAEVKKGYENYNIKLQLEKIIDSYTFSHKILGDIHVDIVRGNIQGQDVNIHFGHAVNNDPNSVFIIR", "aa_hexdigest": "3cfede7f7622c1bc51710ce2563fbba8", "start_type": "ATG", "rbs_motif": "TTATT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7680.6253, "isoelectric_point": 6.381336402893066 }, "id": "NKOCPLAKPK_925", "locus": "GCA003260355_00889" }, { "type": "cds", "sequence": "contig_1", "start": 483936, "stop": 485606, "strand": "-", "frame": 2, "gene": null, "product": "Metalloprotease TldD/E C-terminal domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EGX5", "UniRef:UniRef90_A0A328EGX5" ], "nt": "ATGGAAAAAAAAGAAAGTGTAAATTATTTACCAATTGTTCAAGCTATAGAACAGTTGGTGCAGAGGGCAGTATCGCTGAAAATCAAGAAAGAGAATCCTAATGGCGATATTACAAGCCATACGATTTCTGGTATCAGGATTGCCTATCTGAAAAAGGAGTCTTTTGAAATTACCTATGCTAATGGTATTATGACTCCAATGAAAAAGAATGAGTCGGCTCCCGAGCTTTTGCTTACTCTCTTCTTTGCTAATGAGAAGAAGGAAGCAAGTGTATCGATTCCAATGCAAGAGCGTATTCTTGCTCAGCCCGAAGGTCTTCTTTCTTTGGGATGGACAAAGATTACTCTCGCAATAGATGAGTTGATCGATTATTTTTATGCCTCAAAAATAAAGGACAATAAATTTCATCAAGAAGCGCATGGGAATGTGGTTTATTTAGAGGATTCTGTGTTTGAGGTTAGTGCTTTAGAGCCAATTCCGAGCTCTCTTTGTGACCAAATGTTACATGTAGTCCGTGATTGTTATCTTGCAAAACATGTTGCTTTTGTGGATGCGAATTTGACTGCAAAGAGAGAGCTTTGGATCGTTGTAGATTCTTGGGGCACTCGTGTAATCGACCATCGTGATAGGGTTCAATTTGGGCTCACTCCTTATTTTATGAATGGACAGCGGAGGGTTTATCAATCTCAGCTCTCAGGAATGTTTAAGGATTGTGATGCTCTCAGGAACTATCTTAGCAACGAATTGCATCCAAGGCTTCAAGAGGAATTGTTTACGCTTGATCGTAAGCAGTTAGAGAGCGGGATTTACCCTATCCTTCTTGCACCTTCGGCTGTTGGCACGCTTTTTCATGAAGCAATTGCGGGACATATGCTCTCTGGAGCCTATATTGCTGATGGAATTTCTACGGTTTTCAAAGGGAAACTTGGGAAACGGGTTGCAAAAGAAGGCTTTATGGAGGTGTTGAAGCATATTCAGATTTGGGACTGTCCAAGAGATGAGCGTATGCTTGCTGCTTATCAATATGATATGGAAGGAACCAAAGCTCGAAATGTCTGTCTGATTGACAAAGGGAAGGTAGAAGGCTATCTTCTTGATCGCAATTCTGCATTTCGTTTGAAGCTTGAGAATAATGGGCATGCTTTGGCAGGAAGTTTTAATGAGCAGCTGTTTGCTGATTACTTTCTTTTGCCTGAAGCTGTGCTTCCAGAGCCAAGAGTTTCTAATCTAGAGATTCTTGTCGATTCCGACTATTCTTTAGAAGAAATGAAGGCTGATTTCTTTAGGAAATTTGGCTATTATCTTTGGGTAGAGTCAAGTTCTGGAGAAGTTAATGTGGAAACGGGCACTTTTGAGCTTAAGGTTGATGTCGTTGTGAAAGTATATCCCAATGGTAAAAAAGAGTACTTTCATGGCGGAGTGCTTTCTGCAAATTTGACAGATTTTTTATCTGCTATTTGTGCGGTGAGCAATCATTATGGGAAAAATCAGGGATATTGTGGTGCTCCAAGTGGGGTAGTCCCGACAGAAGAGGTGGCTCCTGCTATGGGTATGTATGGAGTTAATTGGGTTCCTTATTCCTTGCCGGGAAAAAATACAATTCTGACGCTTAAACGGGATAAATATGTCCCCAAGGATTGGGAACAGAAAACTTCTTCGTTTGAGATGTGA", "aa": "MEKKESVNYLPIVQAIEQLVQRAVSLKIKKENPNGDITSHTISGIRIAYLKKESFEITYANGIMTPMKKNESAPELLLTLFFANEKKEASVSIPMQERILAQPEGLLSLGWTKITLAIDELIDYFYASKIKDNKFHQEAHGNVVYLEDSVFEVSALEPIPSSLCDQMLHVVRDCYLAKHVAFVDANLTAKRELWIVVDSWGTRVIDHRDRVQFGLTPYFMNGQRRVYQSQLSGMFKDCDALRNYLSNELHPRLQEELFTLDRKQLESGIYPILLAPSAVGTLFHEAIAGHMLSGAYIADGISTVFKGKLGKRVAKEGFMEVLKHIQIWDCPRDERMLAAYQYDMEGTKARNVCLIDKGKVEGYLLDRNSAFRLKLENNGHALAGSFNEQLFADYFLLPEAVLPEPRVSNLEILVDSDYSLEEMKADFFRKFGYYLWVESSSGEVNVETGTFELKVDVVVKVYPNGKKEYFHGGVLSANLTDFLSAICAVSNHYGKNQGYCGAPSGVVPTEEVAPAMGMYGVNWVPYSLPGKNTILTLKRDKYVPKDWEQKTSSFEM", "aa_hexdigest": "29105b3ee1696a8fd781356103ff45e3", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A328EGX5", "query_cov": 1.0, "subject_cov": 0.9099836333878887, "identity": 1.0, "score": 1119.0, "evalue": 0.0, "valid": true, "product": "Metalloprotease TldD/E C-terminal domain-containing protein", "uniref50_id": "UniRef50_A0A328EGX5" }, "pscc": { "uniref50_id": "UniRef50_A0A328EGX5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EGX5" ], "product": "Metalloprotease TldD/E C-terminal domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_926", "locus": "GCA003260355_00890" }, { "type": "cds", "sequence": "contig_1", "start": 485768, "stop": 486007, "strand": "-", "frame": 3, "gene": null, "product": "DUF4181 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EJX3", "UniRef:UniRef90_A0A328EJX3" ], "nt": "ATGCTTATTGCCTATCAGTACGGTTTAAGAGGACTAAGTATTCGAGCGATGCTTCCTTTTTGCCTTATCTGGCTTTTTCTTGTAGGTCTTCCTATTCGGGAGTATCTAAGAAAAAAGATTAGCACTTTAAAGGCGGGGGAGTATCTTAATATTTTAAATTTATTGCTTCTTATAGTTCTTCAATTTCTTTCAGTCTTTATTATGGGATCTGCGATTCTTCTTGTGAAAATTCTTGTATAA", "aa": "MLIAYQYGLRGLSIRAMLPFCLIWLFLVGLPIREYLRKKISTLKAGEYLNILNLLLLIVLQFLSVFIMGSAILLVKILV", "aa_hexdigest": "c7211181d7b8fd3d943c606eaafa9cec", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 485768, "stop": 486190, "observations": { "mutation": [ 486073, 486124 ], "stop": [ 486073, 486124 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EJX3", "query_cov": 1.0, "subject_cov": 0.5642857142857143, "identity": 0.975, "score": 137.0, "evalue": 2.84e-40, "gene_start": 1, "gene_end": 79, "reference_start": 62, "reference_end": 140, "reference_sequence": "MDFEYLAYTVLCIFAFIAVTYFGNTPSFLKKEKVRHEKIGALSPITRLLYLILTWLICALCMLIAYQYGLRGLSIRAMLPFCLVWLFLVGLPIREYLRKKISTLKAGEYLNILNLLLLTVLQFLSVFIMGSAILLVKILV" }, "paralog": false, "identity": 0.9428571428571428, "score": 258.0, "evalue": 4.72e-90, "description": "Internal stop codon at 486073, 486124. Nonsense mutation around 486073, 486124.", "psc": { "uniref90_id": "UniRef90_A0A328EJX3", "product": "DUF4181 domain-containing protein", "uniref50_id": "UniRef50_A0A328EJX3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EJX3", "UniRef:UniRef50_A0A328EJX3" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EJX3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EJX3" ], "product": "DUF4181 domain-containing protein" } }, "id": "NKOCPLAKPK_927", "locus": "GCA003260355_00891" }, { "type": "cds", "sequence": "contig_1", "start": 486321, "stop": 486461, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCCAAATGAAATCAGAAAAAGCAGAAATAGAAAGCTGATTGAGATCGGCGATCAGGTTCGTGATGCCTTTGTCGCTCAAAACTATGGGAAAAAACTCCAAGTCCTGATTGAAGAAGTGAAAAATGGGAAAGCGAAATGA", "aa": "MPNEIRKSRNRKLIEIGDQVRDAFVAQNYGKKLQVLIEEVKNGKAK", "aa_hexdigest": "bb824ade76391c7dcb70f000f90ac6e9", "start_type": "GTG", "rbs_motif": "TTCTT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5326.1433, "isoelectric_point": 9.991777610778808 }, "id": "NKOCPLAKPK_928", "locus": "GCA003260355_00892" }, { "type": "cds", "sequence": "contig_1", "start": 486591, "stop": 487463, "strand": "-", "frame": 2, "gene": null, "product": "putative protein family UPF0004", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MNA5", "UniRef:UniRef90_A0A328EK21" ], "nt": "ATGCAGCACAAAGTCTACGGTTGTAAAGTCAATAAATTCTACCTCAATAAACGAATCGCTCACCTTGAAGCAACCAATCAAGTGAATGATAATCTTTTTCTCGTTGCGACCTGCGTGGTGACCGATAGAGCCAAGATGAAATGGGTCAAAGAAATCATTGAACAAGCCAAACTTGGCAAGCAAATCCTTATCGCTGGTTGTGGCTCTATCACCAAAGGGAAAGCCACGGAAAGATCAGCTTTTTTTGCCTATTATCCTGAATTAGCACCTTATCAAGAGCAAATCACGCTTCTTGCAGAAGATCCGCTAGAGGAGGAGAGCGAACTCGCCTTTAGTTTCAAAATGCCGAATCAGAAGCTCCGAACCAAGAACTATGTGATCATTCAGAACGGTTGCGATAATCATTGCAGTTTCTGTCTCACGATTTTCAAGAGGGGAGCGCATAGAAATCGCCCTTTGCCGGAGATTATTGAGGAGATTAGACAGATTGAAGCGGAAGGTGGGAAAGAGATCGTGCTGACCGGGATCAATCTTGCAGCTTGGGGTGCGAGCAATACGAGGAATGCTAAGGAAAGCAGATTCAACGAGCTTTTGGAAGCGATCTTGGAGCAAACCACGATCCCAAGGATCAGGATTTCTAGTCTTGACCCTCAGTATCTTGATGATCGTTTTTTTGAGCTGATGAATAATCAGAGGTTCTGTCCGCATTTCCATCTCTCCATTCAGAGTTTTTCTGATGCGGTGCTGAAGCTGATGAATAGAGCCTATGATAGCAAAAAGCTTGATGAAGTCCTAAAAAAGTTAAGAAATCTGAATCGCCCTGATGCGGAACTTGTTTCCATCGGTGCAGATCTTATTTCTGGCTTTCCCTGA", "aa": "MQHKVYGCKVNKFYLNKRIAHLEATNQVNDNLFLVATCVVTDRAKMKWVKEIIEQAKLGKQILIAGCGSITKGKATERSAFFAYYPELAPYQEQITLLAEDPLEEESELAFSFKMPNQKLRTKNYVIIQNGCDNHCSFCLTIFKRGAHRNRPLPEIIEEIRQIEAEGGKEIVLTGINLAAWGASNTRNAKESRFNELLEAILEQTTIPRIRISSLDPQYLDDRFFELMNNQRFCPHFHLSIQSFSDAVLKLMNRAYDSKKLDEVLKKLRNLNRPDAELVSIGADLISGFP", "aa_hexdigest": "ed623f14db83de0fc6207d2240f3bb3f", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 486288, "stop": 487463, "observations": { "mutation": [ 486323, 486593 ], "stop": [ 486323, 486593 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EK21", "query_cov": 1.0, "subject_cov": 0.7038834951456311, "identity": 1.0, "score": 579.0, "evalue": 4.09e-207, "gene_start": 1, "gene_end": 290, "reference_start": 1, "reference_end": 290, "reference_sequence": "MQHKVYGCKVNKFYLNKRIAHLEATNQVNDNLFLVATCVVTDRAKMKWVKEIIEQAKLGKQILIAGCGSITKGKATERSAFFAYYPELAPYQEQITLLAEDPLEEESELAFSFKMPNQKLRTKNYVIIQNGCDNHCSFCLTIFKRGAHRNRPLPEIIEEIRQIEAEGGKEIVLTGINLAAWGASNTRNAKESRFNELLEAILEQTTIPRIRISSLDPQYLDDRFFELMNNQRFCPHFHLSIQSFSDAVLKLMNRAYDSKKLDEVLKKLRNLNRPDAELVSIGADLISGFPGETEADFEITCEAAQKYRITKLHAFPFSDHHKAERIPASSLPDQVPNEIRKSRNRKLIEIGDQVRDAFVAQNYGKKLQVLIEEVKNGKAKGWTENYLQISLSGNYEKGSLVEVIVDEKNCVF" }, "paralog": false, "identity": 0.9948849104859335, "score": 776.0, "evalue": 4.09e-287, "description": "Internal stop codon at 486323, 486593. Nonsense mutation around 486323, 486593.", "psc": { "uniref90_id": "UniRef90_A0A328EK21", "product": "Uncharacterized protein family UPF0004", "uniref50_id": "UniRef50_A0A660MNA5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EK21", "UniRef:UniRef50_A0A660MNA5" ] }, "pscc": { "uniref50_id": "UniRef50_A0A660MNA5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MNA5" ], "product": "tRNA (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-methylthiotransferase" } }, "id": "NKOCPLAKPK_929", "locus": "GCA003260355_00893" }, { "type": "cds", "sequence": "contig_1", "start": 487491, "stop": 487682, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAACTTACAGATATTATGATTTTTATTTTAAGATTAAAAATTGAGGGAAGGTTTGATCATCCCTTATTTAAAATATTTTCTTATTTTATTTCTAAGTATTTTGAGGGCTTGAATATTGATGTAACAAATTTTAATTTGCAAGATTTTTTTGGATATGATCCAAAATATAATTTTTTTCAATCCAATTAG", "aa": "MQLTDIMIFILRLKIEGRFDHPLFKIFSYFISKYFEGLNIDVTNFNLQDFFGYDPKYNFFQSN", "aa_hexdigest": "c263ce8b7fa260cc579080e08ac50ea2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7701.781200000001, "isoelectric_point": 5.554044914245605 }, "id": "NKOCPLAKPK_930", "locus": "GCA003260355_00894" }, { "type": "cds", "sequence": "contig_1", "start": 487944, "stop": 488216, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGATATTTAATTACAGATTCCGATTCTAGAGAATTTGATTTTTTGAATAAACTTAAACAAGCAAGGAAAGAGTCTCCTATGTGTTGGAACACTATCCATGGTTCAGAAATTTCTCAAAATGATGAAAATAAAATAGAGTTAATTAAAAGGTGGTTAGATTTATTTATACAAGAGAAGTCTGTTTCTTTTTATAGTTTTTTGTATAAAAAGAACTTACAGTTTGTCACTAATAATGCCAATAATGGAGGATATGAAAGATATTTTTCATGA", "aa": "MGYLITDSDSREFDFLNKLKQARKESPMCWNTIHGSEISQNDENKIELIKRWLDLFIQEKSVSFYSFLYKKNLQFVTNNANNGGYERYFS", "aa_hexdigest": "a7454a35cd4545074c15faba1c2f7ddf", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10744.94029999999, "isoelectric_point": 6.563789558410644 }, "id": "NKOCPLAKPK_931", "locus": "GCA003260355_00895" }, { "type": "cds", "sequence": "contig_1", "start": 488285, "stop": 488404, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAGAGAAAGAGCACAACAAAATAGATCAGAGCTCTTTATCCATAATAGGGAAGGGAAAATTAGAGAAAGAAACAGTTATTGAAAAGATCCGTATCCTCCTAAATGATAGTGGATGA", "aa": "MQEKEHNKIDQSSLSIIGKGKLEKETVIEKIRILLNDSG", "aa_hexdigest": "3447dc4a049111350b7f845d4bfec05f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4423.0525, "isoelectric_point": 6.544464302062988 }, "id": "NKOCPLAKPK_932", "locus": "GCA003260355_00896" }, { "type": "cds", "sequence": "contig_1", "start": 489071, "stop": 489247, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTGTAAGAGATACAAATAATAAGATAAAAATTCGTATACAAGATGATGGAAAGTTTGTAGTTGGCAATCCTAGTCAAGCTGGTGTTTTTCTTAAATTGATGGATGATGACTATGTAAAATCTATGTTGACAGGAAATCAGTATGATGCTGAGAAAAAGAATAAAATAGAATAA", "aa": "MFVRDTNNKIKIRIQDDGKFVVGNPSQAGVFLKLMDDDYVKSMLTGNQYDAEKKNKIE", "aa_hexdigest": "8b7b0c4ce086558fb0896fd54bf06592", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6667.558100000002, "isoelectric_point": 7.984812355041504 }, "id": "NKOCPLAKPK_933", "locus": "GCA003260355_00897" }, { "type": "cds", "sequence": "contig_1", "start": 489368, "stop": 489544, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATGATCAATTCCAAACGACAGATGCAATACGTTTTAATTATAAGCCTATATTTCTATATTTTAACAATAAAATTCTTATTCTGGATTTTAAAAAATTAGAAAAAGATTTTGGCTATGAAACAACTATGAATCAAAGAGCTTGTGAAAAAATATCAATGTTTACAAGGCAATGA", "aa": "MDDQFQTTDAIRFNYKPIFLYFNNKILILDFKKLEKDFGYETTMNQRACEKISMFTRQ", "aa_hexdigest": "5c5439bf581b0ebebeffbce1362af557", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7111.117, "isoelectric_point": 7.842981910705567 }, "id": "NKOCPLAKPK_934", "locus": "GCA003260355_00898" }, { "type": "cds", "sequence": "contig_1", "start": 490281, "stop": 490397, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACACAGCGTCCTATTAAAATATTAAAGGTTAAAACTAATAATCATGTATATTTAAGTTATTTTATGACTTATATCATTCCTTTTGTTTTTGTTTCATTTGAGAGTACAGATTGA", "aa": "MTQRPIKILKVKTNNHVYLSYFMTYIIPFVFVSFESTD", "aa_hexdigest": "642974a61aa7dbbace618305ba0a451c", "start_type": "ATG", "rbs_motif": "TTTA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4572.3473, "isoelectric_point": 9.302803993225098 }, "id": "NKOCPLAKPK_935", "locus": "GCA003260355_00899" }, { "type": "cds", "sequence": "contig_1", "start": 490632, "stop": 490991, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF21018.2" ], "nt": "ATGATTTCGTCTGCCACCGGACAGGCGATGATGTATGGAATCTTCAAACTCCAGGAGAGAGGACCAATCTTCGTAGACCCAGCTCAGCCAATCTATGAAGGAATGATCGTAGGGGAGCATCTGAAGGGAGGAGATATGACCGTAAACCTTACTATCAATAAGCAAATGAACAATGTCCGCAACTCTGGAAATGATGAAGCGATGAGAATTGATCCAATCGTGCATTTGTCTCTTGAGCAGGCGATGGGATATATCGGACTTGATGAACTGGTTGAGATCACGCCAAAGGCGATTAGAATTAGAAAGAAGTATTTGACTGAGTCTGCGAGGACAATTGCGAGGAAGCAGGGATTGATTTAG", "aa": "MISSATGQAMMYGIFKLQERGPIFVDPAQPIYEGMIVGEHLKGGDMTVNLTINKQMNNVRNSGNDEAMRIDPIVHLSLEQAMGYIGLDELVEITPKAIRIRKKYLTESARTIARKQGLI", "aa_hexdigest": "d86d919df2ba0d318b11b5102251a271", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF21018.2", "name": "BipA_C", "length": 106, "aa_cov": 0.8907563025210085, "hmm_cov": 1.0, "evalue": 1.6005535252850402e-41, "score": 136.9892578125, "start": 1, "stop": 106 } ], "seq_stats": { "molecular_weight": 13262.332299999984, "isoelectric_point": 8.006280326843264 }, "id": "NKOCPLAKPK_936", "locus": "GCA003260355_00900" }, { "type": "cds", "sequence": "contig_1", "start": 491061, "stop": 491288, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00679.30" ], "nt": "GTGATTTACAAAGAAGAAAACGGACAAAAACTTGAGCCAATGGAAAACCTTGTCGTTAGCGTAGAAGACGGGCTTTCTGGAACAGTAATTGATATGCTCGCACCTCGTAAAGGACTCATGCAAAATATGAGCTCAGAAAACGGACTTACAACGATGGAATTTATCATCCCAACCAGAGGTCTTCTCGGATTCCGTGGAGAATTCATCCTCGCGACGAAGGGTGAATGA", "aa": "MIYKEENGQKLEPMENLVVSVEDGLSGTVIDMLAPRKGLMQNMSSENGLTTMEFIIPTRGLLGFRGEFILATKGE", "aa_hexdigest": "68d89b16635a206f5dca3a36a4f3811a", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00679.30", "name": "EFG_C", "length": 67, "aa_cov": 0.88, "hmm_cov": 1.0, "evalue": 1.4527031485263796e-15, "score": 53.53350067138672, "start": 10, "stop": 75 } ], "seq_stats": { "molecular_weight": 8277.523999999994, "isoelectric_point": 4.721467399597167 }, "id": "NKOCPLAKPK_937", "locus": "GCA003260355_00901" }, { "type": "cds", "sequence": "contig_1", "start": 491298, "stop": 492068, "strand": "-", "frame": 2, "gene": "typA", "product": "translational GTPase TypA", "db_xrefs": [ "GO:0003924", "GO:0005525", "GO:0006412", "GO:0006950", "SO:0001217", "UniRef:UniRef50_Q6F0Z6", "UniRef:UniRef90_A0A7W4EB55" ], "nt": "ATGGTTGTACTTAATAAAATTGACAAACCTGCGGCAAGGCCTGACCGAGTGATTGATCAGCTTTTTGATCTGTTTATTTTGCTTGGAGCGAGCGATGAGCAGGCAGATTTCCCGATTATTTATGCGAGTGCAAAAAATGGCTATGCGATGAAAAATCTGACTGATGAAGCGAAGGATTTGACTCCGCTTTTTGATGCGATCTTGGAAAATGTAGCTCCAGCAGCTGAAAAATCTGATCTCCCTTTCTGTATGCAGGTTGCGAACCTCGGATATGATGATTATTTGGGAAGACTTGGAGTTGGGCGTGTGTATCAAGGAACTTTGAACGCAGGAAGCCAGGTAACGATCATCGGAAATGATGGAGAAAAGAGAACTGGGAAGATCGCGCATATTTTTAGAACGCTCGGGCTCCAGAGGATTGAGATTCCTCAGGCAGAGTGTGGTGATATCGTGACTATCAGTGGAATCCCAAATATTTTCGTGGGAGAGACGATCGGAGTAGGGGAGTTTAAGGCATTACCGACAATTGCGATTGATGAGCCGACCTTGAGTATGGAGTTTTTGGTGAATGATAGCCCTTTTGCTGGGAAAGATGGGAAATATATGACGACCAGAAACATGGCAGAAAGACTAGAAAAAGAAGCTGAAACCAATGTTGGTTTGAAAGTAGAAATGGTGGAAGGAAAATTCATCGTCTCAGGAAGAGGAGAGCTTCATCTTGGAGTATTGATTGAAGCGATCAGGAGAGAAGGATGGGAGCTGCAAGTTTGA", "aa": "MVVLNKIDKPAARPDRVIDQLFDLFILLGASDEQADFPIIYASAKNGYAMKNLTDEAKDLTPLFDAILENVAPAAEKSDLPFCMQVANLGYDDYLGRLGVGRVYQGTLNAGSQVTIIGNDGEKRTGKIAHIFRTLGLQRIEIPQAECGDIVTISGIPNIFVGETIGVGEFKALPTIAIDEPTLSMEFLVNDSPFAGKDGKYMTTRNMAERLEKEAETNVGLKVEMVEGKFIVSGRGELHLGVLIEAIRREGWELQV", "aa_hexdigest": "dbd1e48ce9c76c62cbc2e2ee3002cb07", "start_type": "ATG", "rbs_motif": null, "genes": [ "typA" ], "truncated": "both", "pseudogene": { "start": 490995, "stop": 492368, "observations": { "mutation": [ 491021, 491063, 491300, 492080, 492347 ], "stop": [ 491021, 491063, 491300, 492080, 492347 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A7W4EB55", "query_cov": 1.0, "subject_cov": 0.4280936454849498, "identity": 0.988, "score": 499.0, "evalue": 2.64e-173, "gene_start": 1, "gene_end": 256, "reference_start": 121, "reference_end": 376, "reference_sequence": "MAIRNIAIIAHVDHGKTSLVDQLLRQSGTLKKMEEQDLVMDNNDQERERGITIYAKNTAIEYQGERINIVDTPGHADFGSEVERVLRMVDSVILVVDAYEGPMPQTKFVLKKSLELGLKPMVVLNKIDKPAARPDRVIDQLFDLFILLGASDEQADFPIIYASAKNGYAMKNLTDEAKDLTPLFDAILENVAPAAEKSDLPFCMQVANLGYDDYLGRLGVGRVYQGTLNAGSQVTIIGNDGVKRSGKIAHIFRTLGLQRIEIPQAECGDIVTISGIPNIFVGETIGVGEFEALPTIAIDEPTLSMEFLVNDSPFAGKDGKYMTTRNMAERLEKEAETNVGLKVEMVEGKFIVSGRGELHLGVLIEAIRREGWELQVGAPQVIYKEENGQKLEPMENLVVSVEDGLSGTVIDMLAPRKGLMQNMSSENGLTTMEFIIPTRGLLGFRGEFILATKGEGIMYSAFSHYEPFKGEIVKRQVGSMISSATGQAMMYGIFKLQERGPIFVDPAQPIYEGMIVGEHLKGGDMTVNLTINKQMNNVRNSGNDEAMRIDPIVHLSLEQAMGYIGLDELVEITPKAIRIRKKYLTESARTIARKQGLI" }, "paralog": false, "identity": 0.9824945295404814, "score": 874.0, "evalue": 0.0, "description": "Internal stop codon at 491021, 491063, 491300, 492080, 492347. Nonsense mutation around 491021, 491063, 491300, 492080, 492347.", "psc": { "uniref90_id": "UniRef90_A0A7W4EB55", "gene": "typA", "product": "translational GTPase TypA", "uniref50_id": "UniRef50_Q6F0Z6", "go_ids": [ "GO:0003924", "GO:0005525", "GO:0006412", "GO:0006950" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A7W4EB55", "UniRef:UniRef50_Q6F0Z6", "GO:0003924", "GO:0005525", "GO:0006412", "GO:0006950" ] } }, "id": "NKOCPLAKPK_938", "locus": "GCA003260355_00902" }, { "type": "cds", "sequence": "contig_1", "start": 492078, "stop": 492332, "strand": "-", "frame": 2, "gene": null, "product": "GTP-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI003144FC7E" ], "nt": "ATGGAAGAGCAAGACCTCGTTATGGACAATAACGATCAGGAAAGGGAGAGGGGGATCACCATTTATGCCAAAAATACCGCGATTGAATATCAAGGCGAGAGAATCAATATCGTAGATACCCCAGGACATGCGGACTTTGGAAGCGAGGTAGAGAGAGTATTGAGAATGGTGGATTCTGTAATCCTCGTAGTGGATGCGTATGAAGGGCCGATGCCGCAGACCAAGTTTGTGCTTAAAAAGAGTCTTGAACTCTGA", "aa": "MEEQDLVMDNNDQERERGITIYAKNTAIEYQGERINIVDTPGHADFGSEVERVLRMVDSVILVVDAYEGPMPQTKFVLKKSLEL", "aa_hexdigest": "9f6cdaa7203001f577750d4e63338360", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI003144FC7E", "query_cov": 0.8928571428571429, "subject_cov": 0.9493670886075949, "identity": 0.653, "score": 95.1, "evalue": 4.67e-24, "valid": false, "product": "GTP-binding protein", "uniref50_id": "UniRef50_UPI003144FC7E" }, "pscc": { "uniref50_id": "UniRef50_UPI003144FC7E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI003144FC7E" ], "product": "GTP-binding protein" }, "genes": [], "id": "NKOCPLAKPK_939", "locus": "GCA003260355_00903" }, { "type": "cds", "sequence": "contig_1", "start": 493176, "stop": 493466, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAACGCTTCAATCCTACAATAAACCTCGAACCTTTTATATCGCTTCATTTTTGCTCTCTGCGGTTTTTTTCTTTGCTTGTGCCTATCTAAGTCACCAAGAGCAAACAAGATCGGTTCTTATCTTAGAAAATAGCTTAGCAATTATTGGAGTCAGTATGCCGGCTTTGATAGCATTTCGATTGATCAAAAAAGACCCTATCCTTTTGCAAGATCTCAAAGCAAGAGTCACACGTTTTGATAAAAGTTTTTGGAAATATCTTTTGGTAATCTTAGGACTTACTTTTTGA", "aa": "MQTLQSYNKPRTFYIASFLLSAVFFFACAYLSHQEQTRSVLILENSLAIIGVSMPALIAFRLIKKDPILLQDLKARVTRFDKSFWKYLLVILGLTF", "aa_hexdigest": "461da691d62191df4ef24430f2af539a", "start_type": "ATG", "rbs_motif": "TTCTT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11094.106799999983, "isoelectric_point": 9.840728187561034 }, "id": "NKOCPLAKPK_940", "locus": "GCA003260355_00904" }, { "type": "cds", "sequence": "contig_1", "start": 494463, "stop": 495083, "strand": "+", "frame": 3, "gene": "grpE", "product": "Protein GrpE", "db_xrefs": [ "KEGG:K03687", "SO:0001217", "UniParc:UPI000DC04953", "UniRef:UniRef100_A0A328EJY2", "UniRef:UniRef50_A0A7W4HN57", "UniRef:UniRef90_A0A328EJY2" ], "nt": "ATGCCACAATCAACAAACCCTAATGATGAAAAGCAACTTACACCTGAAATAGATACAGAATCAGCCGATCTTGAGGCAAAATTAGAAGAGGTGAAAAAAGAAACACAAGATGCTCAAGCTGATTTTACACCACTTTTGAATAAAATCAAAGAGCTTGAAGCCCAGCTTCAAGAAAAAGATGAAATTGCAAGGAATAGTCAGATTGCTTATTTAAATTTGAAAGCGGATTTTGATATTCTTCAAAGACAAACTCAGCAAAAAATTGAAACTGCGGATCGTGATGCTATTCTGAAGGTTGTAAAGGATATGCTTCCTTTTATTGAGAACCTCAGAAAATCCCTCCTCAACCTCTCAGATGAGGCAAAATCTTCTCCTATGGGACAGGGGCTTCAAATGATGTATGAGAATCTTTTAAGAAGTTTAGAAAAGTTAAAAGTCAGACCAATTGAAGCGACAGGATTGGCTCCTGACGCGCAGTTTCATGAACCTGTAAGTATGCAACCAACGGAAGACGAGCATCTTAAAGGGAAAATTATTCAAGAGTTTGAGCAGGGGTTTATTTATGAAAATGGAGATGACAAACTTGTCATCACTCCATCCAAAGTTATTGTTGGGAATTAG", "aa": "MPQSTNPNDEKQLTPEIDTESADLEAKLEEVKKETQDAQADFTPLLNKIKELEAQLQEKDEIARNSQIAYLNLKADFDILQRQTQQKIETADRDAILKVVKDMLPFIENLRKSLLNLSDEAKSSPMGQGLQMMYENLLRSLEKLKVRPIEATGLAPDAQFHEPVSMQPTEDEHLKGKIIQEFEQGFIYENGDDKLVITPSKVIVGN", "aa_hexdigest": "4d608899049b7d54ed6cd2f3ca912ddc", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000DC04953", "uniref100_id": "UniRef100_A0A328EJY2", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DC04953", "UniRef:UniRef100_A0A328EJY2" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EJY2", "uniref90_id": "UniRef90_A0A328EJY2" }, "psc": { "uniref90_id": "UniRef90_A0A328EJY2", "product": "Protein GrpE", "uniref50_id": "UniRef50_A0A7W4HN57", "kegg_orthology_id": "K03687" }, "pscc": { "uniref50_id": "UniRef50_A0A7W4HN57", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7W4HN57" ], "product": "Protein GrpE" }, "genes": [ "grpE" ], "id": "NKOCPLAKPK_941", "locus": "GCA003260355_00905" }, { "type": "cds", "sequence": "contig_1", "start": 496029, "stop": 496313, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGAAGTATTTAGTCAATCTGCATCAGAAATTCAGGAAATAGCATGGGTAGTTGACAATGTAAAATCTGAATTGCAAGAGTTGAAAAAGGAATTTTTCTTCGAAAAAGATGACAAAGAGGTAAGATATAATATGAATGCCATAAAAGATTATCTTTCTTTTCTAAAAGATAAGTCCTGGAATGAGCTTGCAAAAGAGCATCAATTTCCTGCGACGATGGCAGTCCAAATTGCACTCAGAAAGCTTGGATATCAAAGTGGGAAAGTAGATGGTATCTTATGA", "aa": "MKEVFSQSASEIQEIAWVVDNVKSELQELKKEFFFEKDDKEVRYNMNAIKDYLSFLKDKSWNELAKEHQFPATMAVQIALRKLGYQSGKVDGIL", "aa_hexdigest": "e4de9db6d700847ea7bfd158ca07089e", "start_type": "ATG", "rbs_motif": "TTATT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10992.438999999993, "isoelectric_point": 5.4149599075317365 }, "id": "NKOCPLAKPK_942", "locus": "GCA003260355_00906" }, { "type": "cds", "sequence": "contig_1", "start": 496907, "stop": 497008, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAGGAGTATCTAAATAAAATAGGACTAGACAGGGAACAAATCTATTTTGAGAACATTCTTAATATTGACATTGAAGGCTTCTTAACGAAGAACGGGTGA", "aa": "MEEYLNKIGLDREQIYFENILNIDIEGFLTKNG", "aa_hexdigest": "7bb281bb193b58dd72ea89ff7557deef", "start_type": "ATG", "rbs_motif": "TTATT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3933.396200000001, "isoelectric_point": 4.250954246520997 }, "id": "NKOCPLAKPK_943", "locus": "GCA003260355_00907" }, { "type": "cds", "sequence": "contig_1", "start": 497108, "stop": 497200, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCTACTTATCTAGGACAGAATTTTCTCAAAGATGGCTCTACAATTAATTTTATTCGTGATTCAATTCAGAAACTTTATCAAGAATCTTGA", "aa": "MSTYLGQNFLKDGSTINFIRDSIQKLYQES", "aa_hexdigest": "0c7c903a6262314e77817463fcb7e979", "start_type": "ATG", "rbs_motif": "TTTA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3497.8824000000004, "isoelectric_point": 5.876151466369628 }, "id": "NKOCPLAKPK_944", "locus": "GCA003260355_00908" }, { "type": "cds", "sequence": "contig_1", "start": 497494, "stop": 497619, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTATAAGGTTTGCTCCTCTTTCAGATACCGAAATGCAGCAGTATTTAATTCAAGTTTTGGGAGAATCTGAATCTCCAGAAAAGACGCAGCTCCTTGCAAAAATTGCACTAGGAAGACCTTGA", "aa": "MVIRFAPLSDTEMQQYLIQVLGESESPEKTQLLAKIALGRP", "aa_hexdigest": "187ecc849bc560bf81541f0a6a068e26", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4602.3324999999995, "isoelectric_point": 5.002479362487793 }, "id": "NKOCPLAKPK_945", "locus": "GCA003260355_00909" }, { "type": "cds", "sequence": "contig_1", "start": 497647, "stop": 497817, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13177.12" ], "nt": "GTGAGGGAGTTGACAAATCGGTTACAGCAGTCTTCTTTTAGTGGTAAAAAGGTGCTTCTTGTGGAAAATATTCAAAGAATGACGAATGCTGCAATGAATGCTTTCCTAAAAACTTGTGAAGAGCCACTCCCTAATAGATATCTCTTTGCTACAGCAGAGCATGAAAGCTGA", "aa": "MRELTNRLQQSSFSGKKVLLVENIQRMTNAAMNAFLKTCEEPLPNRYLFATAEHES", "aa_hexdigest": "e3cefcc919addbe86d878b9872cdef2f", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13177.12", "name": "DNA_pol3_delta2", "length": 54, "aa_cov": 0.9464285714285714, "hmm_cov": 1.0, "evalue": 4.379754036507546e-12, "score": 42.736846923828125, "start": 2, "stop": 54 } ], "seq_stats": { "molecular_weight": 6458.3431999999975, "isoelectric_point": 7.941683006286622 }, "id": "NKOCPLAKPK_946", "locus": "GCA003260355_00910" }, { "type": "cds", "sequence": "contig_1", "start": 498346, "stop": 498660, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGGGCTAAAAGAATAGTTGGTCTTATCATTTTGCTTGGATATTTAGCGTTTAATGTTCTTTCTTATCTAGGATATCTTGATTGGAATAGGGCATCTGCATCTTCCGATAATATGAATACCAATACTCAATTGGTTGCACTTTTGGTTGATGAGAAAATCTATCCAAGTATTGAAGCTGATCTTAAACGATATTCAACTCAGTATATTCAAAAACATTATCCACAAAGCAAGGCTCTGGTCCTGAAAATTAATACGAGCGAATATGATGCTCCAGAAATTGCAAAACTACTTGAAAATCTCTATTTTGACTGA", "aa": "MRAKRIVGLIILLGYLAFNVLSYLGYLDWNRASASSDNMNTNTQLVALLVDEKIYPSIEADLKRYSTQYIQKHYPQSKALVLKINTSEYDAPEIAKLLENLYFD", "aa_hexdigest": "1db064d05a4d96c3c25e6b75f58389a5", "start_type": "ATG", "rbs_motif": "TTATT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11950.613199999989, "isoelectric_point": 6.562766456604003 }, "id": "NKOCPLAKPK_947", "locus": "GCA003260355_00911" }, { "type": "cds", "sequence": "contig_1", "start": 498700, "stop": 499128, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGGAACTATTCCATTTCCTGCAGTTAGGTATGATAATTATGTTTTCCCCTCAATCTACCCTTATGTTGATTTTTTGGATCAGAAATATGTTTGGAATCAAGAGCTTGGTTATTTTACTGAAAACAGAAAAGATGGGCAGCCCGAAGTTTGGCATGGAGTAATTGATTTTGATCAGGATATTGATGCCTATCACAAATTTTTTGATAAACTTAAACTCTATGATGCAAAGCCTAAAAAGTTTGTCGCGAAAAAAATATGGTATGATGATTTTATTGCTCATAAGAAAAATTTTCTTGAAGAAACCTATCAACTCTATCAGAATAAGCTTCTTTTTGCAGAAGATTTTATCTATCATCGTTATACTGATCTTTTTAACCGCATTTTGCAAGGGGATCAGTATAAAGAACAGGCTTTACAGCTTTCTTGA", "aa": "MGTIPFPAVRYDNYVFPSIYPYVDFLDQKYVWNQELGYFTENRKDGQPEVWHGVIDFDQDIDAYHKFFDKLKLYDAKPKKFVAKKIWYDDFIAHKKNFLEETYQLYQNKLLFAEDFIYHRYTDLFNRILQGDQYKEQALQLS", "aa_hexdigest": "72a1e3d7d35a6482fc8c6065ee2196b2", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17370.386199999986, "isoelectric_point": 5.5172132492065415 }, "id": "NKOCPLAKPK_948", "locus": "GCA003260355_00912" }, { "type": "cds", "sequence": "contig_1", "start": 499348, "stop": 499791, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCATACTATTTCTGAGAATGTTAAAGCAGGAGATAGATGGACTGGCAATGATAGTCATTATCAAAAACTCCAGCTCAAAGATCAGCTCCTTCTTGGGAACACTAAACTTAATGGGATTATTAGAAGCATTAATGATAAGCTTGAGTCCTTTGTGGATGAAAAAGTAGAAAAGGAAAAATATGCTATGAAAAGTACTATTCCTTATTATTATAAAGAAAGTCAGATTCAGAAAAAAGCAGCTTTTTCTAAGGATTTTATGTATTCTTATCAAGATTATACTATAAATAACATCTATAGATTTTTTTATTTTGGGAAAGACGCACAGACAATTACTAAAGCTGTAGATTATTCTACCTATAGAGGAACTTATAGGAATTTAAGCGGCCTCGTAAACTATCCTTCCATCATCCAAGATCAACAAAATCCAGCAAAGTCGGATTGA", "aa": "MHTISENVKAGDRWTGNDSHYQKLQLKDQLLLGNTKLNGIIRSINDKLESFVDEKVEKEKYAMKSTIPYYYKESQIQKKAAFSKDFMYSYQDYTINNIYRFFYFGKDAQTITKAVDYSTYRGTYRNLSGLVNYPSIIQDQQNPAKSD", "aa_hexdigest": "eb8a4293f9db7789dde2daeac631bc0c", "start_type": "ATG", "rbs_motif": "TTATT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17236.127799999987, "isoelectric_point": 9.046606636047361 }, "id": "NKOCPLAKPK_949", "locus": "GCA003260355_00913" }, { "type": "cds", "sequence": "contig_1", "start": 500089, "stop": 500301, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATAAATATCAAAATCAGATTTATGCCTTTAGTGGAGAATATGACTTTAGACGTGCTACAAATGAAATTTTTGATATTGGAGGCTCTGTTCCATTAACAGGAGAAGAAAAAGAGGCGCCTCATTTTCTCGCGGAGAAAATCTATGTCTCTCCTATTCTAATTGCAGATAAGGATGGCTTTCATTGGGATAAAACTATGTATGATACCTGA", "aa": "MDKYQNQIYAFSGEYDFRRATNEIFDIGGSVPLTGEEKEAPHFLAEKIYVSPILIADKDGFHWDKTMYDT", "aa_hexdigest": "42f9be90fd368f0113de11ffdfb5bafa", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8124.942599999998, "isoelectric_point": 4.60369701385498 }, "id": "NKOCPLAKPK_950", "locus": "GCA003260355_00914" }, { "type": "cds", "sequence": "contig_1", "start": 500803, "stop": 501150, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAGAGTATTGTTTTTAGATCAATTGTTCGAACTAGGAAAATTGATATTTTCTCTGAATGGCATGCAGACATCCTCAAGGATTTTGATAAGTTGATTAAGTTTTTACTTGAAAAAAAAGCTATTGCTTCACAAATTTTTAAGCTTTATCGTGAGGTGATTAATCAACTTGATGGTATTGATGCAATTATTAGCTCAGGAATTGCCAATAATAGCAAAGAAGAACAACAAGCTGTGAGAGAAGCATGGACAAAACTTCGTTCTCCATTTGCTAAATTATCTGCCATTGTTCATGAGATCAATACTGATACAGAAGATTCATGTATTTGTAATCATGGAATAGTTTGA", "aa": "MQSIVFRSIVRTRKIDIFSEWHADILKDFDKLIKFLLEKKAIASQIFKLYREVINQLDGIDAIISSGIANNSKEEQQAVREAWTKLRSPFAKLSAIVHEINTDTEDSCICNHGIV", "aa_hexdigest": "ebabf4a6ee9452bc62f779d89b29c6df", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13176.042999999983, "isoelectric_point": 6.825930976867675 }, "id": "NKOCPLAKPK_951", "locus": "GCA003260355_00915" }, { "type": "cds", "sequence": "contig_1", "start": 501259, "stop": 501405, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTGAAGCATTAAAAGAGAGTAAAGAGGATTTTAAAGAAGACTTCAATTCAGATCCTGAAAATCCAGAGATCCTTTACACAGGCATGAACATCCTCACACCAAGTAGGCCTATTGATTCTCCAAGATATCTTTCCTTTCAATGA", "aa": "MIEALKESKEDFKEDFNSDPENPEILYTGMNILTPSRPIDSPRYLSFQ", "aa_hexdigest": "3b83db86b401ec62a4ce31366defc831", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5607.1943, "isoelectric_point": 4.31762638092041 }, "id": "NKOCPLAKPK_952", "locus": "GCA003260355_00916" }, { "type": "cds", "sequence": "contig_1", "start": 501511, "stop": 501678, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAAGTCCGGAAAGTATTCGCAAAGCCCTTAGTCAATATTTAAGCAATAAAGTAAAGGAATACAATGCTATCATCAATGCTGAAAAAGCAAAGGCAAAACCAATGAATAAGCATTATCAGTATATTTCTGTTGTTGATAAATTAGGAACCCCTCTTCTTGACTGA", "aa": "MKSPESIRKALSQYLSNKVKEYNAIINAEKAKAKPMNKHYQYISVVDKLGTPLLD", "aa_hexdigest": "feec9ed8e1c52f97ca5f07c507024ce1", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6266.249600000003, "isoelectric_point": 9.647516441345214 }, "id": "NKOCPLAKPK_953", "locus": "GCA003260355_00917" }, { "type": "cds", "sequence": "contig_1", "start": 501877, "stop": 501981, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATAATTATCTCTCACAGAAAAGAGATGCTTTTTATGAAGAGAAAAATGCTGATCCAAAATTTGTCATTCCTAGTTATGCCTCAAAAGGTTATGAGGTGTGA", "aa": "MDNYLSQKRDAFYEEKNADPKFVIPSYASKGYEV", "aa_hexdigest": "797728f84c2b854b38f4f862398b3950", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4004.3897000000006, "isoelectric_point": 4.992987251281736 }, "id": "NKOCPLAKPK_954", "locus": "GCA003260355_00918" }, { "type": "cds", "sequence": "contig_1", "start": 502735, "stop": 502914, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTTGCAGTCCCTGGGCCCGTTCATAGCTTTGAAATAGGTCTTCCTAACGATAGAAAAATACTTGCTGGAGCTTATAGCCTTATCTCTCTTAAAGCTTTTGATCAATATAAAAATGAAATTCAGAGAACTCTAGAACCATATACTATTGAGGTTGATAGAGGTGGGCTTATTGTTTGA", "aa": "MFAVPGPVHSFEIGLPNDRKILAGAYSLISLKAFDQYKNEIQRTLEPYTIEVDRGGLIV", "aa_hexdigest": "ffc400d77e7bb7ebb33cbf0154099e24", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6592.531199999999, "isoelectric_point": 5.6171361923217775 }, "id": "NKOCPLAKPK_955", "locus": "GCA003260355_00919" }, { "type": "cds", "sequence": "contig_1", "start": 503158, "stop": 503430, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAGTAAATGAGAAAAAAGCAATCCCCCTTGATCTTGTTCTAAGGGATAATAATGGTAGAGAAATTCCTATTACTACCAATGTTATCTTGGGTGCGCAAGAAGAATTGCTTCAATTTATGGTGCTAAAAAAATCTACTGATGAAAATGGCAAGCAATGGCTGAATTTTTCTAATACAAATTCTCTCTTTGTAGAAAAAGGAAGAGCAAAAGTATATCTAACCTCAAAAAATATTGCTGGGGATGAAGAAATTTCTTCGCTTATTCCGTGA", "aa": "MEVNEKKAIPLDLVLRDNNGREIPITTNVILGAQEELLQFMVLKKSTDENGKQWLNFSNTNSLFVEKGRAKVYLTSKNIAGDEEISSLIP", "aa_hexdigest": "683ca4759a4d14b0c8f2871234620e30", "start_type": "ATG", "rbs_motif": "TTCTT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10119.453699999995, "isoelectric_point": 5.097059440612792 }, "id": "NKOCPLAKPK_956", "locus": "GCA003260355_00920" }, { "type": "cds", "sequence": "contig_1", "start": 503929, "stop": 504216, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTAGATCTTAATAAAATCAAAAAAGCGAGTGTTATCTTAGGTAAAAACTCTTTGATCGAGAACAATGATAATCTTGATATTCAGGCAACTCAAGAAGCAGGTCAGCTTTCTTTAACTCTTGCCAAGATCGGTAGAATTCGTATTATGGATTCTTTTAAGAAAATTAGAGTAGTCTCTGCTGATCCTGAGAATAAAAGACTTGTCCAAGAGCTGAAAAAGGATAACGGGAAATCAATGTATCTCTATGATTTGAATGATGATTTCACCTATAGAAAGGATAAGTGA", "aa": "MVDLNKIKKASVILGKNSLIENNDNLDIQATQEAGQLSLTLAKIGRIRIMDSFKKIRVVSADPENKRLVQELKKDNGKSMYLYDLNDDFTYRKDK", "aa_hexdigest": "21eef2a59dfef305cabe76766ef9fc82", "start_type": "TTG", "rbs_motif": "TTTA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10887.441299999988, "isoelectric_point": 9.361921501159667 }, "id": "NKOCPLAKPK_957", "locus": "GCA003260355_00921" }, { "type": "cds", "sequence": "contig_1", "start": 504526, "stop": 504618, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAAAGAATATTGGATTTAGGGGAGAATTTAAAAATGTTACTTTGTTTGCTGGAGGTAAGACCGTTGGTGAGGCTACACTCCCTTTTTGA", "aa": "MEKNIGFRGEFKNVTLFAGGKTVGEATLPF", "aa_hexdigest": "15c95bcdf084da81753188ca91a2c9ec", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3259.7303, "isoelectric_point": 8.2548059463501 }, "id": "NKOCPLAKPK_958", "locus": "GCA003260355_00922" }, { "type": "cds", "sequence": "contig_1", "start": 504796, "stop": 504990, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13517.12" ], "nt": "GTGGTCTACAAAGATGGGACTATTAGGCTTCAGAAACAGTATCAGGATAAGAGATTTCGTGATATGGAGACTTTAATGGTCAGCGCAGAACAAATTAAGGATATTTTTGTTGGTGATGTAGATGGGAATAAATATGAGGATATTATTATTAGGAATACCAAGGATCAACTCAGAATTTATACTAATGAAAATTGA", "aa": "MVYKDGTIRLQKQYQDKRFRDMETLMVSAEQIKDIFVGDVDGNKYEDIIIRNTKDQLRIYTNEN", "aa_hexdigest": "38b44239d9e312a52d5a3e02f7793cf8", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13517.12", "name": "FG-GAP_3", "length": 47, "aa_cov": 0.71875, "hmm_cov": 1.0, "evalue": 8.103369782686587e-08, "score": 29.264968872070312, "start": 5, "stop": 50 } ], "seq_stats": { "molecular_weight": 7704.642299999999, "isoelectric_point": 5.260983085632325 }, "id": "NKOCPLAKPK_959", "locus": "GCA003260355_00923" }, { "type": "cds", "sequence": "contig_1", "start": 505472, "stop": 505714, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCGTGTAGTACTAATGCTAATTGAGAAAAGGTTTGTAAGAGCTATAAACGTAAAAGTGCAGAACCTACCTGTACTCAGCCAGCAGATAAACAAAGCGATGTTTGCTGTGCTCAAAAGGGTGAAGGAAGTAAAGAAGAAAAATGTAAACTCTCTAAATGACGTCTTGACAAAAAGACTGAACAAGAAATTCATAGATGAGATACCGATTTTGACTTTACTGATATGGCTCAAGAGGGATTAA", "aa": "MRVVLMLIEKRFVRAINVKVQNLPVLSQQINKAMFAVLKRVKEVKKKNVNSLNDVLTKRLNKKFIDEIPILTLLIWLKRD", "aa_hexdigest": "7148d1a9cdc58259f9bfed9a937cd7a8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9454.488299999994, "isoelectric_point": 10.87325382232666 }, "id": "NKOCPLAKPK_960", "locus": "GCA003260355_00924" }, { "type": "cds", "sequence": "contig_1", "start": 505696, "stop": 505839, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTCAAGAGGGATTAAGAGTCTTTGATAAGTACGTTAAGAATCCATTTGACAAGATTGTAAGTTTTGATGATGGCGTTAAACCATCAGAGCAAGCCTTTTTGACTATTAATCAAGTTGATTCAAAAGATCCAATACTTTGA", "aa": "MAQEGLRVFDKYVKNPFDKIVSFDDGVKPSEQAFLTINQVDSKDPIL", "aa_hexdigest": "09aee874e51e69018bac61e3a207322d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5344.012500000001, "isoelectric_point": 4.663718986511231 }, "id": "NKOCPLAKPK_961", "locus": "GCA003260355_00925" }, { "type": "cds", "sequence": "contig_1", "start": 506137, "stop": 506478, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGATCCATTATTTAAAATCAGACTTGAAGATGCCGAGATTACCGATTATAGCGCACCTCCAAAAATAAAGAGAACTGCACCGAAAGATGGTGAAAATGCCGATAAGCTGCCAGATTTTATTGTTCAATCTGTGGATGGCTGTAACAAGAAAGAATCTATCCTCTTTAATAATAAATCTGATGCTAAGAGGAGTTATCGTGAGTCCGTTATCGATCTTCAAGAACTAAGTGATGTATATTCTAAGGCTATTGAAGAGAATAAGACTAGGACAATGGATAAGGCGAAATCTGACATTCAAGACAGTAAGACTGTGGAAAAGATGAATGCTATCCCGTGA", "aa": "MNDPLFKIRLEDAEITDYSAPPKIKRTAPKDGENADKLPDFIVQSVDGCNKKESILFNNKSDAKRSYRESVIDLQELSDVYSKAIEENKTRTMDKAKSDIQDSKTVEKMNAIP", "aa_hexdigest": "67c241fb87a5c93a92a90fb6c2d7614b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12825.296699999988, "isoelectric_point": 5.251036262512206 }, "id": "NKOCPLAKPK_962", "locus": "GCA003260355_00926" }, { "type": "cds", "sequence": "contig_1", "start": 506539, "stop": 506730, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGAGGACTCCTCTGTTTCTGTAGAGCTCTTCTCAGACGAGACCTCAGAAATTGAAAAGACAGTGCAAGATATCGCAGATAAAGCATGTAATGGATTCAGTTTTGGACTTTCTAATGTTGGATCCTGTGAAGTTCCGGTGCCATTTAATCAGGCATTCTTAGCTCCTGGGAAATATCATCTGATGTGA", "aa": "MKEDSSVSVELFSDETSEIEKTVQDIADKACNGFSFGLSNVGSCEVPVPFNQAFLAPGKYHLM", "aa_hexdigest": "3a0a2dafa996416ac8ab51786a7cbf4a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6845.5838, "isoelectric_point": 4.313931846618654 }, "id": "NKOCPLAKPK_963", "locus": "GCA003260355_00927" }, { "type": "cds", "sequence": "contig_1", "start": 507610, "stop": 507855, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGCAAAAAATACATGGTCATCACTTCAGTCTAAAGAACAACTTACAGCTTTGGATATTGCGAATGGAGACAACCCCTTTGAGAAACTTCAGTCTTTCTTTAATAGTAAAGAACTTGTAAATATTAGAACCCAAAAAATTCATGTCAAAATTCCTTGGATCTATAGCGAAGATATTGAGGCATATAAAAATTACTTGCTTACATGGATGGAAACGAACGGAGAGGTTTTGAAGGCCTGGGAGTGA", "aa": "MAKNTWSSLQSKEQLTALDIANGDNPFEKLQSFFNSKELVNIRTQKIHVKIPWIYSEDIEAYKNYLLTWMETNGEVLKAWE", "aa_hexdigest": "e786397cfacc06e34bce3b8db6075ecf", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9537.705699999997, "isoelectric_point": 5.359087181091309 }, "id": "NKOCPLAKPK_964", "locus": "GCA003260355_00928" }, { "type": "cds", "sequence": "contig_1", "start": 507961, "stop": 508686, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAGACAAAGCTTGATAAAACAAGTGAACAGATTTATCAGAATATTCAAACTCTTGAGCTTTATAAGAGATTTCCACTAGAGATTTATGAGTGGCTTCACGTATCTGATAGGTACTTAACTGAGATATCATCACTCATTTCGAAATTCTTTGGCTATATTAATTATTGGATGGATGTTAATGCCAATAGATTCTCTCAATATGTTGATGCTATTATTACGATTATTGCGGTTGTGAAGACTTATCAGGTAATGGTTGATCTTTTTGTGGATCGAGGAAAGAAATGTGGTAAATGTACGCAAGATTCTTACGATCAATATTCTTGTAAGCTCAGTATGCTTTGTCCAAATCTACCTATCATACCGATCCCTAATGTAAAAATCCCAAGTCTCTATGTCGATCTTTCTCATCTCAATCTTGGATTAGATATTGTGCTTCCTCGTTTTAATTTTGTACCAGAATCTATTGAGCTTCCAAGACTTCCAAACCTTCCAGAGCCGCCAAGATTAGGGCTTAACATTAATGTCGACTTTAATATTCCAGATATTCCTCAACTTCCTGAGCCTCCAAACTTACCAGAATTGCCATCATTCATTCCTCAAGTAAAAATGGAATTGCCGATTCTTCCTCCTGCTCCTAAGATTCCTGAATTGCCTAACAAAATTCAGAAAATTGTCAGTATTGCTAAGAAAATCTCTAAGATTTACTGTATTATCAAGTCATGA", "aa": "MQTKLDKTSEQIYQNIQTLELYKRFPLEIYEWLHVSDRYLTEISSLISKFFGYINYWMDVNANRFSQYVDAIITIIAVVKTYQVMVDLFVDRGKKCGKCTQDSYDQYSCKLSMLCPNLPIIPIPNVKIPSLYVDLSHLNLGLDIVLPRFNFVPESIELPRLPNLPEPPRLGLNINVDFNIPDIPQLPEPPNLPELPSFIPQVKMELPILPPAPKIPELPNKIQKIVSIAKKISKIYCIIKS", "aa_hexdigest": "ef6d7d9442c9067eeb7eee4a11e52a72", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 27757.395200000035, "isoelectric_point": 7.556880378723145 }, "id": "NKOCPLAKPK_965", "locus": "GCA003260355_00929" }, { "type": "cds", "sequence": "contig_1", "start": 508837, "stop": 509157, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAATTTACAGTATAATTTTGATGCGTTCTACGCACTTATGAAGGGTATTGTAAATAATGTTAACCAAAAGACCAATAATCTCGTTTCTCAAGGGCAAGAAGTCTCTGATGCTCTAAGTACTGCGAGTGATACCGCAACAAATACTTTAAATTCAGCTACAAGTTCACCAATTCAAGTAAATGTTGGTTATCAAAAGCAATTAAAAGATAGCCTTAAAGACGTTCAAGCAATTCTCTCACCACACCAGGAGAAAGAAAAACTTGATTCGATTATTGCTCTTGCCTCAACTGATGGCGAAGTAAAGAAAGACTCTGATTGA", "aa": "MNLQYNFDAFYALMKGIVNNVNQKTNNLVSQGQEVSDALSTASDTATNTLNSATSSPIQVNVGYQKQLKDSLKDVQAILSPHQEKEKLDSIIALASTDGEVKKDSD", "aa_hexdigest": "0943d0daf6e2ac0e5a2db58d3d329255", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11506.599499999993, "isoelectric_point": 4.778647422790528 }, "id": "NKOCPLAKPK_966", "locus": "GCA003260355_00930" }, { "type": "cds", "sequence": "contig_1", "start": 509227, "stop": 509619, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTGCAAAGTCAGACTACTCTACTTTTCTGAAAACCTTGGAAGGAGCAAGTAAAAAAGAACCACTTCAGCTCACGTTTTCTAGTAGCCTGCTGGCGGAAAACAAAGGTGCGGAACATGCGATTGCAGGCATAAATCCTACTGAAATGATTATTCATACGGAAGGAGAAAGACTTCAGGGTTATCTCTCAGCATTAAAATCTCATAGTCCAGAAAGTTTGAATATGTCGAGTGAGAATTATAATAAATCAAAGTCCTATCTTGAAGAGGTCCAATCGGCTCTTAATCAGCGAAAGAGCATTAAACAGAAAACTTATGCAGGAAATCAACCACTCCTTACTAAAAATGGCACAGAAACTAATAGAACGCTTTTGACGCAGACTTCTCAATGA", "aa": "MLAKSDYSTFLKTLEGASKKEPLQLTFSSSLLAENKGAEHAIAGINPTEMIIHTEGERLQGYLSALKSHSPESLNMSSENYNKSKSYLEEVQSALNQRKSIKQKTYAGNQPLLTKNGTETNRTLLTQTSQ", "aa_hexdigest": "d4c480bfac7da6e37d799aa66b2b6f31", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14365.951999999981, "isoelectric_point": 8.804398918151858 }, "id": "NKOCPLAKPK_967", "locus": "GCA003260355_00931" }, { "type": "cds", "sequence": "contig_1", "start": 509653, "stop": 509940, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAGTAGCTATATAAATGGGATCCTTGTGAAAGATCATAGCAATAATATGGTAAATGTTGTGCATTCCAAGGTTAACTATGAGAGGTTCTTTGACTATTATCAAAAAGATATTAATAATGATAAAAAACCTGAAGTCATAACTCGGGATGAATTTAATGTCTACGTAAAGTATGCAGACGATGTGGAACCAAAAATGAAACAGCAGTTTACAAAATATTATGTTTTAACTCCAAACCTAAAAAATAAAAATAAAAAATACGAATCCTTTGGAGGAACACTTTTCTGA", "aa": "MSSYINGILVKDHSNNMVNVVHSKVNYERFFDYYQKDINNDKKPEVITRDEFNVYVKYADDVEPKMKQQFTKYYVLTPNLKNKNKKYESFGGTLF", "aa_hexdigest": "b1653fc1f927ad10b7d1e8d9f9da48a6", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11376.743099999992, "isoelectric_point": 9.00238132476807 }, "id": "NKOCPLAKPK_968", "locus": "GCA003260355_00932" }, { "type": "cds", "sequence": "contig_1", "start": 509953, "stop": 510063, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAAACTTTACGATCAAAATTGGGAGGTAAAGAATTTTAGACTTGACGGTCAGACCTTTGATACGATCAGTTTTTCTTGGGATAACAATAAAACGGAAGATATTGCCTGA", "aa": "MKLYDQNWEVKNFRLDGQTFDTISFSWDNNKTEDIA", "aa_hexdigest": "8d2613f9f64ac3ec6f4eb085b3bfa8b1", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4370.719, "isoelectric_point": 4.32961940765381 }, "id": "NKOCPLAKPK_969", "locus": "GCA003260355_00933" }, { "type": "cds", "sequence": "contig_1", "start": 510100, "stop": 510384, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAGAAGAATGACCTCAAAAATGTAAAATATGCCCTTTTCCTACCTAATGAGACTCCTCTCGATCACCTTAAGCTGAAGGCTGATGATATAAGTTTCTCTGTAAAAAGTCAGATTGGAAAGCTAATAAATGAAGTTCAATACTACAATCCTGCAAATGATGTATTCCAGTTTCAGCTTCACCAGCTTCCTAGAAAATGGCAATACTTACAAGTTACTACCTTAAAGAAACAAGCTACACTTTATGAGCAAAATGCTCCACGATCTAATCAAGTTGTAGGATGA", "aa": "MEKNDLKNVKYALFLPNETPLDHLKLKADDISFSVKSQIGKLINEVQYYNPANDVFQFQLHQLPRKWQYLQVTTLKKQATLYEQNAPRSNQVVG", "aa_hexdigest": "d1e8fd9d295a12640b4a15721f8ac1c1", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10993.435099999995, "isoelectric_point": 9.18824367523193 }, "id": "NKOCPLAKPK_970", "locus": "GCA003260355_00934" }, { "type": "cds", "sequence": "contig_1", "start": 510532, "stop": 510825, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTCGTCGGGCAAAAATTGAAGAGAATGGGAAAACGCTTGCTCAAAAGGATATTAATGCTCCTAGTGGCACGATCACATTGAAAAACCTCTTCTTTACTGAAGCTCAGGTGAGAAATTATGTAATATCAGCTATTGATAGTCAAGACAATGAGAAAGAAGAAGAGGTTACTCTCGAAATTAATATTCCTAAGATTACCATTGAGGATATTCAGCGTTTTTCTGGATGGAAAGAAGGAATTGAAAATCCGGTTTTAATCTCTTCAGCACTAGAAAGAGATATTGATAAGTGA", "aa": "MVRRAKIEENGKTLAQKDINAPSGTITLKNLFFTEAQVRNYVISAIDSQDNEKEEEVTLEINIPKITIEDIQRFSGWKEGIENPVLISSALERDIDK", "aa_hexdigest": "568d7733e77f901f3c46552c4a35e89b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11018.30489999999, "isoelectric_point": 4.757105445861816 }, "id": "NKOCPLAKPK_971", "locus": "GCA003260355_00935" }, { "type": "cds", "sequence": "contig_1", "start": 511003, "stop": 511305, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAATGGTCATAATGGAGAAATCATTGTTCAGCCTGCATACAAGAAATCTGTTTCTCTAGGAGTAGACTTTTCAAAAGGCTATCCTGTTGTAAAGGTAGTGAGTGATGCCAAAGTTCTCTTCGAAGTTGTCCTAAAATCAGAGCAACTCCTTAGCAAAGAAATCTCTGATGGAACACTCTTCCCTCTAGAGGGAAAGGATTTTGGCATATTTGATGGTGGAGAGGTCGTGATGAAAGGGAAGGAACCACTTCTCTATATTGCTCCAGACGGATCGCTTCGAAGTAGTAAGCCACTGCAGTGA", "aa": "MNGHNGEIIVQPAYKKSVSLGVDFSKGYPVVKVVSDAKVLFEVVLKSEQLLSKEISDGTLFPLEGKDFGIFDGGEVVMKGKEPLLYIAPDGSLRSSKPLQ", "aa_hexdigest": "af9c2100debb978a861978db9d1b4bc2", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10811.391099999992, "isoelectric_point": 5.770033073425293 }, "id": "NKOCPLAKPK_972", "locus": "GCA003260355_00936" }, { "type": "cds", "sequence": "contig_1", "start": 511479, "stop": 512153, "strand": "+", "frame": 3, "gene": null, "product": "Imelysin-like domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MY59", "UniRef:UniRef90_A0A660MY59" ], "nt": "ATGAAAAAATCAGTTTTCACCTTTTTGTTTGGGATTCTTCTCCTGTTTGGAATCACTTCTGCACATGATTGCCATCTTTTTCCTGAACTCTCAGTAAAAAACCTAGAAAAAACGTTATTTGAAAGCTCAGTAAAAGGAAATCCAGCATTAGCGACACTCACTGAGGAAGGATTACGTAGGGCTCTTATCAATTTGAAGGCTCATTGTTGCGCATCTCAGATTTTGGGTGATAATGCACAAATGCTTGCTTCCTGTAAGAAAGATGCAAAATACCTAACTGAAAGAACAAATTACCCTCAGTCTTCTTTTCTTTTTGATCATGTTATTGATGTGATGATGAGGAGACTGATGGTAGACGGGAAATATGAAGAACTCCCTCTTGACAAAAAAGCTCAGGAATGGAGAGAAAAGACTGATCGTATCGCTGATGGAGCGGATGGAGTTATTCCAGTTGCGATTTCCAAGGATTATAAAAAATTTTGGGGACTTCAACCTGAGTTTCTTATTCCTGAATATAATGGGGTTTCTGCTCCTGAATATAAAAAAGCGATTAATGATTTAGAAAGCAAGAAGGCAATTTTTGCCAAATATGAAGATTGGAACTTAAATACGAGATATTATAATCTTTGTCAAAGTGCGATCTATTTGATGACTCATCTACCAATAGATTTTTGA", "aa": "MKKSVFTFLFGILLLFGITSAHDCHLFPELSVKNLEKTLFESSVKGNPALATLTEEGLRRALINLKAHCCASQILGDNAQMLASCKKDAKYLTERTNYPQSSFLFDHVIDVMMRRLMVDGKYEELPLDKKAQEWREKTDRIADGADGVIPVAISKDYKKFWGLQPEFLIPEYNGVSAPEYKKAINDLESKKAIFAKYEDWNLNTRYYNLCQSAIYLMTHLPIDF", "aa_hexdigest": "3de50f74cef1ad6889b906d604178ea2", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 511479, "stop": 512408, "observations": { "mutation": [ 512151, 512316 ], "stop": [ 512151, 512316 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A660MY59", "query_cov": 1.0, "subject_cov": 0.7249190938511327, "identity": 0.996, "score": 457.0, "evalue": 1.3e-161, "gene_start": 1, "gene_end": 224, "reference_start": 1, "reference_end": 224, "reference_sequence": "MKKSVFTFLFGILLLFGITSAHDCHLFPELSVKNLEKTLFESSVKGNPALATLTEEGLRRALINLKAHCCASQILGDNAQMLASCKKDAKYLTERTNYPQSSFLFDHIIDVMMRRLMVDGKYEELPLDKKAQEWREKTDRIADGADGVIPVAISKDYKKFWGLQPEFLIPEYNGVSAPEYKKAINDLESKKAIFAKYEDWNLNTRYYNLCQSAIYLMTHLPIDFGSEQMALAQKKCSSMTQQNIMNEITNLSRLISHKSELLLAQNMENYADAYLMDTRGGKFEEKSQRMLSNLFGVMRMVPKLISMCN" }, "paralog": false, "identity": 0.9870550161812298, "score": 612.0, "evalue": 3.48e-225, "description": "Internal stop codon at 512151, 512316. Nonsense mutation around 512151, 512316.", "psc": { "uniref90_id": "UniRef90_A0A660MY59", "product": "Imelysin-like domain-containing protein", "uniref50_id": "UniRef50_A0A660MY59", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A660MY59", "UniRef:UniRef50_A0A660MY59" ] }, "pscc": { "uniref50_id": "UniRef50_A0A660MY59", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MY59" ], "product": "Imelysin-like domain-containing protein" } }, "id": "NKOCPLAKPK_973", "locus": "GCA003260355_00937" }, { "type": "cds", "sequence": "contig_1", "start": 512163, "stop": 512318, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTCTTGCACAGAAAAAATGCTCCTCTATGACCCAGCAAAATATCGTAAATGAAATCACCAATCTTTCAAGACTCATTTCTCATAAATCTGAGCTTCTTTTGGCACAGAATATGGAAAACTATGCAGATGCATATCTTATGGACACAAGATGA", "aa": "MALAQKKCSSMTQQNIVNEITNLSRLISHKSELLLAQNMENYADAYLMDTR", "aa_hexdigest": "c7d5a5e974fcb585970f67b370971602", "start_type": "ATG", "rbs_motif": "TTTTT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5831.6575, "isoelectric_point": 6.523547554016113 }, "id": "NKOCPLAKPK_974", "locus": "GCA003260355_00938" }, { "type": "cds", "sequence": "contig_1", "start": 512598, "stop": 512945, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EGQ5", "UniRef:UniRef90_A0A328EGQ5" ], "nt": "GTGAAAGATGTGGCAAATAGAACAAAAGGAACTGTAATTGATACCTATAATGAAATTGCTAGTGGATACGAATCTAGAGGAGAAATCGGGAAAGCTATGCAATCCGGGATTATGGGTTGGAATACCTTGATTCAGTATGTAGTTTATGCTATGAGTTTTCTTTCTCAATTAGGACTGCTTATTGGGGGGCTTATGATTCTCTATGCAGGATACCTTTATGGATCAACCGTTTTTTCTGGAAGCAATCCAACAAAAGCAAAAAGTGCAATACGTAATGCTATTATTGGTGTTTTGGTTGTGGTCTTTTCTTTTGCCATCTGGAAAGGACTTGTGAGCAGTTTTCTCTAG", "aa": "MKDVANRTKGTVIDTYNEIASGYESRGEIGKAMQSGIMGWNTLIQYVVYAMSFLSQLGLLIGGLMILYAGYLYGSTVFSGSNPTKAKSAIRNAIIGVLVVVFSFAIWKGLVSSFL", "aa_hexdigest": "45eee48bf5f71051131a4c94a2cf86e0", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 512430, "stop": 512945, "observations": { "mutation": [ 512460, 512499 ], "stop": [ 512460, 512499 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EGQ5", "query_cov": 1.0, "subject_cov": 0.672514619883041, "identity": 0.991, "score": 221.0, "evalue": 3.03e-72, "gene_start": 1, "gene_end": 115, "reference_start": 57, "reference_end": 171, "reference_sequence": "MKLILKLFVCGMLCIYGFMSPEVGLLSFAQSPSTTAKTLHIIPENSESDEKQVKQDVKDVANRTKGTVIDTYNEIASGYESRGEIGKAMQSGIMGWNTLIQYVVYAMSFLSQLGLLIGGLMILYAGYLYGSTVFSGSNPTKAKSAIRNAIIGVLVVVFSFAIWKGLVSSFL" }, "paralog": false, "identity": 0.9824561403508771, "score": 325.0, "evalue": 1.28e-115, "description": "Internal stop codon at 512460, 512499. Nonsense mutation around 512460, 512499.", "psc": { "uniref90_id": "UniRef90_A0A328EGQ5", "uniref50_id": "UniRef50_A0A328EGQ5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EGQ5", "UniRef:UniRef50_A0A328EGQ5" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EGQ5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EGQ5" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 12402.367499999986, "isoelectric_point": 9.476095008850098 }, "id": "NKOCPLAKPK_975", "locus": "GCA003260355_00939" }, { "type": "cds", "sequence": "contig_1", "start": 513372, "stop": 513722, "strand": "-", "frame": 2, "gene": null, "product": "UvrABC system protein B", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MVD0", "UniRef:UniRef90_A0A660MXL9" ], "nt": "ATGATTGAAACCTATCTCCAAAAAAAACTAAATGAAGTCAAAAAATCTGACTTTCCCCCTTTTTTGGACCATCTTCTGCAGAAAATCAGAATGCATATCAATAATCAAGGAAAAATTCTCCTTGTTACTTTGACCAAAAAGTCCTCTGAAGAAGTTACAAATTTTCTCGTAGCTCAGGGTTTCAAAGCATTCTACCTGCATAGTGAGATTGCTACTATCGACCGCTGGGAAATAATTAAGAAGCTGAGAACAGGAGAAATTGATATTATTGTCGGTGTTAATCTCCTTAGAGAAGGAATTGATCTTCCTGAAGTGACGATGATCGGGGTTCTTGACGCAGATAAAGAGTGA", "aa": "MIETYLQKKLNEVKKSDFPPFLDHLLQKIRMHINNQGKILLVTLTKKSSEEVTNFLVAQGFKAFYLHSEIATIDRWEIIKKLRTGEIDIIVGVNLLREGIDLPEVTMIGVLDADKE", "aa_hexdigest": "1c7aeb5f9e7be069192e3c47614bf994", "start_type": "ATG", "rbs_motif": "TTTA", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 513069, "stop": 513722, "observations": { "mutation": [ 513125, 513206, 513374 ], "stop": [ 513125, 513206, 513374 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A660MXL9", "query_cov": 1.0, "subject_cov": 0.4566929133858268, "identity": 0.983, "score": 226.0, "evalue": 6.99e-73, "gene_start": 1, "gene_end": 116, "reference_start": 1, "reference_end": 116, "reference_sequence": "MIETYLQKKLNEVKKSDFPPFLDHLLQKIRMHINNQGKILLVTLTKKSSEEVTNFLIAQGFKAFYLHSEIATINRWEIIKKLRTGEIDIIVGVNLLREGIDLPEVTMIGVLDADKEGFLRSTTSLIQIIGRAARNPQSEVVLYADSFTESMIKALRETYRRRRIQELYNTKNGITPKSALSNVKSLETVKDDLSLDQSFGSITKGKVKKLKKATKAEREIILKDLKIQLDDAIKAREFEKAALIRDQIKEISGE" }, "paralog": false, "identity": 0.9769585253456221, "score": 404.0, "evalue": 1.16e-145, "description": "Internal stop codon at 513125, 513206, 513374. Nonsense mutation around 513125, 513206, 513374.", "psc": { "uniref90_id": "UniRef90_A0A660MXL9", "product": "UvrABC system protein B", "uniref50_id": "UniRef50_A0A660MVD0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A660MXL9", "UniRef:UniRef50_A0A660MVD0" ] }, "pscc": { "uniref50_id": "UniRef50_A0A660MVD0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MVD0" ], "product": "UvrABC system protein B" } }, "id": "NKOCPLAKPK_976", "locus": "GCA003260355_00940" }, { "type": "cds", "sequence": "contig_1", "start": 513904, "stop": 514122, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF04542.20" ], "nt": "ATGCATCATAGTGACCGTGAGCTTGTCCTCGCTTTTCAAGCTGGGAAGTATCATTGCTTTGAAACTTTATATGAACGTGTGCTTCCAAAAGTTTACCGCTATCTTCTCTTTAAAACTAATGGAAACAAAACACTAGCTCAAGATCTTTGTTCCGAAACCTTTTTGGCTGGATTTGAGGGATTGGGAAGTTTTCAAGCAGATGAGAACTGTAATTTTTGA", "aa": "MHHSDRELVLAFQAGKYHCFETLYERVLPKVYRYLLFKTNGNKTLAQDLCSETFLAGFEGLGSFQADENCNF", "aa_hexdigest": "6fec7b90130a00a5b151c1920afca844", "start_type": "ATG", "rbs_motif": "TTATT", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF04542.20", "name": "Sigma70_r2", "length": 50, "aa_cov": 0.6944444444444444, "hmm_cov": 0.98, "evalue": 3.772909884418679e-07, "score": 26.323026657104492, "start": 23, "stop": 72 } ], "seq_stats": { "molecular_weight": 8327.335499999997, "isoelectric_point": 5.823916435241699 }, "id": "NKOCPLAKPK_977", "locus": "GCA003260355_00941" }, { "type": "cds", "sequence": "contig_1", "start": 514472, "stop": 514816, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAACTAGATGAACTTCTCAACGAGCATCCTGAACTCAGTAACGAAAAAAAGCAGATTGAAGCTGTAGTTTCTGCATTTGCTGGGAGTAAAATAACTATTGATAAATCTTTTTCTCAGTCTCTTAAATCATCAATTCGCGAGAAGATTAAAGAAAAAAAGGCTCAGAATCCGAATTATTTTTTGTTTTATCTCCGCCATTTTAGGTTTTATTTTACGGGATTTAGTGTAGCATTAGGATGTTTTTTGATCCTTTTTGTGCTCGGATTTTTCAATTTTAATGCTAGGCCTCAAATACTTAACAATCCAAAGCTTCTTGCTTACGTTGGTCCTGAAGCGTTTTGA", "aa": "MKLDELLNEHPELSNEKKQIEAVVSAFAGSKITIDKSFSQSLKSSIREKIKEKKAQNPNYFLFYLRHFRFYFTGFSVALGCFLILFVLGFFNFNARPQILNNPKLLAYVGPEAF", "aa_hexdigest": "03eecf45871d251002eec84529098258", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13160.145099999982, "isoelectric_point": 9.443216133117676 }, "id": "NKOCPLAKPK_978", "locus": "GCA003260355_00942" }, { "type": "cds", "sequence": "contig_1", "start": 515009, "stop": 515275, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAATAGCAACTCTTCTAATATGCAGTTTCTCTCAAAGAATATTAAGCTTCCTGAGTTTTCTCTTTCTCCTTTTAAAAAGGACACCATTACGACCATTGATTTTGAGAATAATCAGCTAGATTATCATTTTACGATCAATCTCGATCAAGGGCTTTTCTCTGCGATAAAAAATAGTGCTTATAATAGTTTGACTGGCTCTGATGTTCCTTTATTATCTGAGAAAGAAATTAAAAAAAGAGTCAAAAGTCAGATTTCAAATTTTTGA", "aa": "MNSNSSNMQFLSKNIKLPEFSLSPFKKDTITTIDFENNQLDYHFTINLDQGLFSAIKNSAYNSLTGSDVPLLSEKEIKKRVKSQISNF", "aa_hexdigest": "f4334983215aaf326ebd25907944eb36", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10023.193799999994, "isoelectric_point": 7.985070228576661 }, "id": "NKOCPLAKPK_979", "locus": "GCA003260355_00943" }, { "type": "cds", "sequence": "contig_1", "start": 515408, "stop": 515620, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAGTTTCTTTTGACCTTGAAAGTCAGGAAATTATCTCACTCTACTCTTTCCAATTTCAGTCCTATGAATTATCCAAATATCCACAGACAAAATCTCCATCCGATATTCTAGAATCTATTTCAAAACTTGGAAATATTAATGTCTCCAAAAATAAAGATGAAGGGGATATTCCTATGAACAAAGGGAAGGAAATTTATCTCCAGAAGTGA", "aa": "MQVSFDLESQEIISLYSFQFQSYELSKYPQTKSPSDILESISKLGNINVSKNKDEGDIPMNKGKEIYLQK", "aa_hexdigest": "586dec6d9137d27d6c84d41ba496f0d5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8050.9836000000005, "isoelectric_point": 4.927224540710449 }, "id": "NKOCPLAKPK_980", "locus": "GCA003260355_00944" }, { "type": "cds", "sequence": "contig_1", "start": 515882, "stop": 516064, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAGCAGCTTTCAGGTCACACTTTGGATGCAAAGCAATTAAAATTTTCACAGGTAGAAAGCTGCGAATCTCTAGAAAAAATGCTCGAGAAATATGCAAAAGAGACTCAAGAACCTCTCTATAGAACTTTTGGGCTCAAAAAAGAACTTGCTGTCGAGAGTGCAGCACCTATGGCTCAATGA", "aa": "MEQLSGHTLDAKQLKFSQVESCESLEKMLEKYAKETQEPLYRTFGLKKELAVESAAPMAQ", "aa_hexdigest": "8fa2f4a7dfb121ed056b0f70641983a6", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6820.7521, "isoelectric_point": 5.297075843811036 }, "id": "NKOCPLAKPK_981", "locus": "GCA003260355_00945" }, { "type": "cds", "sequence": "contig_1", "start": 516329, "stop": 517231, "strand": "+", "frame": 2, "gene": null, "product": "Beta propeller domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EJY8", "UniRef:UniRef90_A0A328EJY8" ], "nt": "GTGACTAAGGGACGTCTGGTTCTCATGGGACAATTGTCTGATTATGGAGACGCTGCGTATGCATCTTTTCTTAGCTATCAAAATCGTACCGTAGTAGCGATCTATGACATTTCTGATATAAAAAATCTCAAGCTTCTAAAATTCGAGAACTTACCTGGAAGTTATCAAGATGCTAGGCTTATTGATGATCAACTTTATGTGATTTCTGAGCTGAGGTTTAATCGATGGAACTGGAAAGGAAAGTCTCATGACTTTAGGAAGGGACTTGCTTCTGTAGAGATTGATGAACAAGGAGCAAAAGTGCTGCCTATAGAATGCTCTCAGATTTCTTATGTTCTGCCAGAAGATAAAAACCTGAGTCTTGATCCAGTTTTTACTCTTATTGCAGGAATTGATATTCGTAATACTTCTAAAAAAGTTTCTACCACTGCCCTTCTTGCGCCCAATGGCGAAATCCATATGAGTAAAAATGCTCTCTATCTGGTTTCAAACTTTTATACCTACAATAATTGGCCATGTCCTTCTGGACTTTTCTGTGTGATGCCGGTTTTCAGAAGCACGACACAAACGATGATTCATAAATTTAGTAGGGATAGATTGAATTTAAAGTATCAAAACACTGCGATTGTCCCAGGTTCCTTGCTGACTCAATACAGTATGGATGAAGATAGTGATGGAAACTTTAGAATCCTGACTAAAGTCTGGGAAAAACATCTTTCAACTCAGCTTTATGTCTTTAAACCAGATCTCACACTTGCTGGGAAACTCGAAAACATTGAGCCAGGAGAAGAATTTAAGGCATCAAGATATATTGGAGACAAGCTTTATTTGGTTACTTTTGAGCAGATCGATCCGCTTTTTGTGGTTGATCTTGCTAATATTAGTCAGCCAAAAATTATTTGA", "aa": "MTKGRLVLMGQLSDYGDAAYASFLSYQNRTVVAIYDISDIKNLKLLKFENLPGSYQDARLIDDQLYVISELRFNRWNWKGKSHDFRKGLASVEIDEQGAKVLPIECSQISYVLPEDKNLSLDPVFTLIAGIDIRNTSKKVSTTALLAPNGEIHMSKNALYLVSNFYTYNNWPCPSGLFCVMPVFRSTTQTMIHKFSRDRLNLKYQNTAIVPGSLLTQYSMDEDSDGNFRILTKVWEKHLSTQLYVFKPDLTLAGKLENIEPGEEFKASRYIGDKLYLVTFEQIDPLFVVDLANISQPKII", "aa_hexdigest": "2b10f6b0cbdce7b0d68dae7837ee6bb6", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 516029, "stop": 517534, "observations": { "mutation": [ 516062, 516143, 517229, 517283, 517337 ], "stop": [ 516062, 516143, 517229, 517283, 517337 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EJY8", "query_cov": 1.0, "subject_cov": 0.42857142857142855, "identity": 0.997, "score": 605.0, "evalue": 6.28e-213, "gene_start": 1, "gene_end": 300, "reference_start": 176, "reference_end": 475, "reference_sequence": "MKKLLFGFSALCMLIGVGANAADTQLLEQLSGHTLDAKQLKFSQVESCESLEKMLEKYAKETQEPLYRTFGLKKELAVESAAPMAQGIAAEAAMTNSISSSHTDFSQTNIQKIGVDEPELLKTDGTYFFYYNEKTQKVSIVASPLDSKTSTLDGTRIQLVNEIAVPKELNDIELFVTKGRLVLMGQLSDYGDAAYASFLSYQNRTVVAIYDISDIKNLKLLKFENLPGSYQDARLIDDQLYVISELRFNRWNWKGKSHDFRKGLASVEIDEQGAKVLPIECSQISYVLPEDKNLSLDPVFTLIAGIDIRNTSKKVSTTALLAPNGEIHMSKNALYLVSNFYTYNNWPCPSGLFCVMPVFRSTTQTMIHKFSRDRLNLKYQNTAIVPGSLLTQYSMDEDSDGNFRILTKVWEKHLSTQLYVFKPDLTLAGKLENIEPGEEFKASRYIGDKLYLVTFEQIDPLFVVDLANISQPKIIGELKIPGYSTYLHPLKKEGSKQYLLGLGYNTTQNERGGTVNSGVKISLFQVDYAKKQGDMIEVKELDSLNQGGKNSDSQVLSNPRLFVMDKKGNVTLPLHLVERAHQGEKCNITYDLQGKEQKKDCTPIVEPSEYFVGLKTFSVSPEKGIKEQFAKDYLKDILAKKGITTTGNSKQIQKEIWNNEEIFSLQDMRVGYAGDALYSFSNLFADFIFPNKKNYSIWFK" }, "paralog": false, "identity": 0.9900199600798403, "score": 975.0, "evalue": 0.0, "description": "Internal stop codon at 516062, 516143, 517229, 517283, 517337. Nonsense mutation around 516062, 516143, 517229, 517283, 517337.", "psc": { "uniref90_id": "UniRef90_A0A328EJY8", "product": "Beta propeller domain-containing protein", "uniref50_id": "UniRef50_A0A328EJY8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EJY8", "UniRef:UniRef50_A0A328EJY8" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EJY8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EJY8" ], "product": "Beta propeller domain-containing protein" } }, "id": "NKOCPLAKPK_982", "locus": "GCA003260355_00946" }, { "type": "cds", "sequence": "contig_1", "start": 517406, "stop": 517675, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATCGAAGTCAAAGAACTTGACTCACTTAACCAAGGAGGGAAAAATTCTGATTCTCAAGTCCTTTCAAATCCAAGACTCTTTGTAATGGATAAAAAAGGGAATGTTACTCTGCCTTTACATCTCGTTGAGCGGGCTCATCAAGGGGAAAAGTGTAACATTACCTATGATCTTCAGGGAAAGGAGCAGAAAAAAGACTGCACTCCTATTGTTGAACCTTCTGAATACTTTGTAGGCTTAAAAACGTTTTCTGTGAGTCCAGAAAAGTGA", "aa": "MIEVKELDSLNQGGKNSDSQVLSNPRLFVMDKKGNVTLPLHLVERAHQGEKCNITYDLQGKEQKKDCTPIVEPSEYFVGLKTFSVSPEK", "aa_hexdigest": "05f848f140c1495e466a84ecf74fd59a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10006.299499999994, "isoelectric_point": 6.083500099182127 }, "id": "NKOCPLAKPK_983", "locus": "GCA003260355_00947" }, { "type": "cds", "sequence": "contig_1", "start": 518148, "stop": 518621, "strand": "+", "frame": 3, "gene": null, "product": "SLH domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EK49", "UniRef:UniRef90_A0A328EK49" ], "nt": "TTGAGGCTTAATAAAAAAGGGCTCACCATCCACAAAACTCGATCAGATTTTTCTCCTGATAAAGCTATTAGAAGAGATGAGGCTGCAAAGATGCTTACCTTAGCGCTCATCCACCTCCCTCAGCCGATTAGCTTTGCTGAGAATAAGGATTGTGTCTTCTCTGATGAGGATCAGGCATGGTCTGATCTTAAGGCAGTCTTGATGCAATCCTGTACAAAAGGGCTTTTTAAAGGGAATAAGGGAAAGTTTAACCCTCAGCTTTCTATTACTAATGGGCAGATTATGACGGTGCTCGGGAGAATGCTCTTTGGGCAACAAGACGAAGGAGAGGGGCATTATGCGACCCGCTATGTAGAACTTCTTGAACAGAAAGGTTATCTTCCTGATAGCTCTTTGAGCGAGGAAAAAAATCGAGACCTTCCTGCAACGAGAGCAACGCTTGCAAAATTACTAGGGAAGGTTTTGAAAGATTAG", "aa": "MRLNKKGLTIHKTRSDFSPDKAIRRDEAAKMLTLALIHLPQPISFAENKDCVFSDEDQAWSDLKAVLMQSCTKGLFKGNKGKFNPQLSITNGQIMTVLGRMLFGQQDEGEGHYATRYVELLEQKGYLPDSSLSEEKNRDLPATRATLAKLLGKVLKD", "aa_hexdigest": "bd8cb0304afa181c9b42f992dce88fc8", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 517977, "stop": 518621, "observations": { "mutation": [ 518022 ], "stop": [ 518022 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EK49", "query_cov": 1.0, "subject_cov": 0.7336448598130841, "identity": 0.9940000000000001, "score": 314.0, "evalue": 1.4e-107, "gene_start": 1, "gene_end": 157, "reference_start": 58, "reference_end": 214, "reference_sequence": "MKKILISLGGILCIFGVGSAFGLDLNSALTSSQQLLNGKGEIKVKAVSTVKDDDELILRLNKKGLTIHKTRSDFSPDKAIRRDEAAKMLTLALIHLPQPISFAENKDCVFSDEDQAWSDLKAVLMQSCTKGLFKGNKGKFNPQLSITNGQIMTVLGRMLFGQQDEGEGHYATRYVELLEQKGYLPDSSLSEEKNRDLPATRATLAKLLGKVLKD" }, "paralog": false, "identity": 0.9953271028037384, "score": 417.0, "evalue": 1.25e-150, "description": "Internal stop codon at 518022. Nonsense mutation around 518022.", "psc": { "uniref90_id": "UniRef90_A0A328EK49", "product": "SLH domain-containing protein", "uniref50_id": "UniRef50_A0A328EK49", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EK49", "UniRef:UniRef50_A0A328EK49" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EK49", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EK49" ], "product": "SLH domain-containing protein" } }, "id": "NKOCPLAKPK_984", "locus": "GCA003260355_00948" }, { "type": "cds", "sequence": "contig_1", "start": 518719, "stop": 519621, "strand": "-", "frame": 1, "gene": null, "product": "Methionine--tRNA ligase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7W4HHF6", "UniRef:UniRef90_A0A660MQ42" ], "nt": "ATGTATGTGCGATGTGATGCTTTGACCAATTATTTGACGGGACAAGGCTTTGGACTGAGCGATAAACGAGAAGCGATTTGGCCAGCTGATGTGCATGTGATCGGGAAAGATATTCTGAGATTCCACGCAGCGTTCTGGCCAGCAATGCTCCTTTCTGCAAAGCTCCCGCTCCCCAAAACCTTGCTTGCCCACGGGCATCTCACCCTAAATGGTGCTAAAATGTCCAAGTCTACAGGAAACGTCCTTGATCCAGAGGCGGTTCTTGAGCAATATGGCAGAGATCCCTTTGTATTCAATTTGCTCTATGATGTTTCTCTCAATGCAGACGGAGATTTCTCTATGGAGAGGCTCGCAAATGTTTATAATAGTATGCTGATCGGTGCTTGGGGGAATCTCGTTAATAGAGTAGTCAGCCTTTGTAGTAAATATGGGATTAATCAGGGAAAAGCTGATCCTGAACTCCTAAGATCACGAAACGAAAGCGATGAAGATTTGAATTTTGAAGGTTTCATTGAGAAGATTGAGGACCGTTATCTCAAGACTTTTGATCTTCAAGGCTACCTTCAGGATTGGTATCGTATCGTTCAAAAAGCAAACGAGTATATCACCAAAGCCGAGCCTCGAAAAAAATGGAAAGAGGAGTCTTCAAAAGCCGATGCTGAAAGGGACTTACAGTTCTTGCTCTATATTGTTAAAAATCTCGCCATACTTTCCGCTCCAATACTTACGGTTGGCTTTGAAAAGTTAAAAAACATTTTAGGAATTGACGCTTTACAGGAGATTGATACCAGCAAAAATCTGAATCTTGCACAGGTGCAGTCAGCTTTCAATCTCAAGGAGTTTACCGTCGACTTGAAGCCAGAAATTTTGTATGCAAGAGTAGAAATTGAGGCAAAGAGCTAA", "aa": "MYVRCDALTNYLTGQGFGLSDKREAIWPADVHVIGKDILRFHAAFWPAMLLSAKLPLPKTLLAHGHLTLNGAKMSKSTGNVLDPEAVLEQYGRDPFVFNLLYDVSLNADGDFSMERLANVYNSMLIGAWGNLVNRVVSLCSKYGINQGKADPELLRSRNESDEDLNFEGFIEKIEDRYLKTFDLQGYLQDWYRIVQKANEYITKAEPRKKWKEESSKADAERDLQFLLYIVKNLAILSAPILTVGFEKLKNILGIDALQEIDTSKNLNLAQVQSAFNLKEFTVDLKPEILYARVEIEAKS", "aa_hexdigest": "1beebb8952f40f667283be1db3768845", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 518719, "stop": 519921, "observations": { "mutation": [ 519639 ], "stop": [ 519639 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A660MQ42", "query_cov": 1.0, "subject_cov": 0.5725190839694656, "identity": 0.987, "score": 588.0, "evalue": 1.05e-208, "gene_start": 1, "gene_end": 300, "reference_start": 225, "reference_end": 524, "reference_sequence": "MEKKFYITTAIAYMNAGPHAGHALEIIQTDALARMWRFLGYEVIFQTGSDDHGMKIRNASKEAGLEVHDFVQQNTQLFKSLYSKLDISYDVFQQTSNEALHYPGAQLMRKKLVEAGDLYKKSYKGLYCEGCEALKLEKDLINGKCPDHPNKELQEIEEENYFFRLSNYKDQVAKLIRDGVYQIEPEMRKNEILAFLEKAEDVSFSRQKAKMPRGIPVPGDEDHVMYVRCDALTNYLTGQGFGLSDKREAIWPADVHVIGKDILRFHAAFWPAMLLSAKLPLPKTLLAHGHLTLNGAKMSKSTGNVLDPEAVLEQYGRDPFVFNLLYDVSLNADGDFSMERLANVYNSMLIGAWGNFVNRVVSLCSKYGINQGKADPELLRSRNESDEDLNFEGFLEKIEDRYLKTFDLQGYLQDWYRIVQKANEYITKAEPRKKWKEESTKADAERDLQFLLYIVKNLAILSAPILTVGFEKLKNILGIDALQEIDTSKNLNVAQVQSAFNLKEFTVDLKPEILYARVEIEAKS" }, "paralog": false, "identity": 0.9875, "score": 796.0, "evalue": 6.88e-293, "description": "Internal stop codon at 519639. Nonsense mutation around 519639.", "psc": { "uniref90_id": "UniRef90_A0A660MQ42", "product": "Methionine--tRNA ligase", "uniref50_id": "UniRef50_A0A7W4HHF6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A660MQ42", "UniRef:UniRef50_A0A7W4HHF6" ] }, "pscc": { "uniref50_id": "UniRef50_A0A7W4HHF6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7W4HHF6" ], "product": "Methionine--tRNA ligase" } }, "id": "NKOCPLAKPK_985", "locus": "GCA003260355_00949" }, { "type": "cds", "sequence": "contig_1", "start": 519637, "stop": 519972, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCGAAAAAAGCTCGTTGAAGCTGGAGATCTCTACAAGAAGTCTTACAAAGGGCTCTACTGCGAGGGCTGTGAGGCACTCAAGCTCGAAAAAGACCTCATCAATGGAAAATGCCCCGATCATCCCAATAAAGAGCTCCAAGAAATAGAAGAAGAGAACTATTTTTTCAGGCTTTCAAACTATAAAGATCAGGTAGCAAAGCTGATCAGGGATGGCGTATATCAGATTGAACCAGAAATGAGAAAAAACGAGATTCTCGCCTTCTTAGAAAAGGCTGAAGATGTCAGCTTCTCTCGTCAAAAGGCTAAAATGCCTCGGGGAATCCCGGTGCCTTGA", "aa": "MRKKLVEAGDLYKKSYKGLYCEGCEALKLEKDLINGKCPDHPNKELQEIEEENYFFRLSNYKDQVAKLIRDGVYQIEPEMRKNEILAFLEKAEDVSFSRQKAKMPRGIPVP", "aa_hexdigest": "cf86e386cc8d68ea044d52d56c73148d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12985.864399999984, "isoelectric_point": 7.641570472717285 }, "id": "NKOCPLAKPK_986", "locus": "GCA003260355_00950" }, { "type": "cds", "sequence": "contig_1", "start": 519982, "stop": 520293, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00133.28", "PFAM:PF01406.26" ], "nt": "ATGGAAAAAAAGTTCTATATTACTACAGCGATTGCTTATATGAATGCAGGTCCTCATGCAGGGCATGCTTTAGAGATTATCCAGACGGATGCTTTGGCAAGAATGTGGAGATTCCTCGGCTATGAGGTGATTTTTCAGACGGGGAGCGACGATCATGGAATGAAAATCCGAAATGCATCCAAAGAAGCAGGACTGGAGGTCCATGATTTCGTCCAGAAGAATACGCAGCTCTTTAAGTCTTTGTATTCCAAGCTCGACATCTCCTACGATGTCTTCCAGCAGACTTCAAATGAAGCATTACATTACCCTTGA", "aa": "MEKKFYITTAIAYMNAGPHAGHALEIIQTDALARMWRFLGYEVIFQTGSDDHGMKIRNASKEAGLEVHDFVQKNTQLFKSLYSKLDISYDVFQQTSNEALHYP", "aa_hexdigest": "fa0bf31aff14c5bf9bcb7a0b1262ed46", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00133.28", "name": "tRNA-synt_1", "length": 57, "aa_cov": 0.5533980582524272, "hmm_cov": 1.0, "evalue": 2.095066373505838e-09, "score": 33.05382537841797, "start": 3, "stop": 59 }, { "id": "PF01406.26", "name": "tRNA-synt_1e", "length": 78, "aa_cov": 0.7572815533980582, "hmm_cov": 1.0, "evalue": 1.2715365351911178e-07, "score": 27.743778228759766, "start": 17, "stop": 94 } ], "seq_stats": { "molecular_weight": 11803.23729999999, "isoelectric_point": 6.167223930358887 }, "id": "NKOCPLAKPK_987", "locus": "GCA003260355_00951" }, { "type": "cds", "sequence": "contig_1", "start": 520379, "stop": 520525, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGACGAAGCTACCCCTGATAACGTAGACGTAGCTAAAAGTACGCGCTATACTATCAAGAAAAAGCTCTTTAGAGGAGATGATTTCTCTAAATATATGGGCGCTGACAGTGAAGACGTCATACTCGAGCCAGTAGAGGTCAAATAA", "aa": "MDEATPDNVDVAKSTRYTIKKKLFRGDDFSKYMGADSEDVILEPVEVK", "aa_hexdigest": "7c661a6b29dcb2716723c957c4dd92e8", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5453.072600000001, "isoelectric_point": 4.654454231262207 }, "id": "NKOCPLAKPK_988", "locus": "GCA003260355_00952" }, { "type": "cds", "sequence": "contig_1", "start": 520498, "stop": 520671, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATCTACTGAGAAAATCTTACCGAAACAGAAGCTGATGAAAAGAGTTCAACCTATGATTGTGAAGATGATCAAGGGAAACCTGACAAATGCCCACGAAAATTCCTCTCAGATGTATTTATTCAGGAGTCAGTTGATTACGGATTGGACGAAGCTACCCCTGATAACGTAG", "aa": "MKSTEKILPKQKLMKRVQPMIVKMIKGNLTNAHENSSQMYLFRSQLITDWTKLPLIT", "aa_hexdigest": "775803802c243471302d9cb8a882c0ab", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6703.037999999999, "isoelectric_point": 10.29284496307373 }, "id": "NKOCPLAKPK_989", "locus": "GCA003260355_00953" }, { "type": "cds", "sequence": "contig_1", "start": 520658, "stop": 521020, "strand": "-", "frame": 3, "gene": null, "product": "PEGA domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EKR0", "UniRef:UniRef90_A0A328EKR0" ], "nt": "ATGAATGAATTTTTAAACGAAGCGAAAGCAGGAGCAAAAGCTTGGCTTGTCAAGGCAGGAACCTCATTAGCAGTGATCCTTCTTGTAGTGATCGGAGCACGAGTATATAGCTCTTCCAAGAGTGCAGAGTCAGTGATTGATAATCCAACAGAGGAAGCGATCACTTTCAAGCTTGATGGAAAAGATTATACCCTAGAGCCAAAAACTTCTCAGGTGATCAAGCTTTCCAAGGGAGAGCACACCCTAGAGTATCTTGGAGAAACGACAAAATTTACCAAGAAAGCTCCTAAGTTTTTGGATACTGACTATTCAATTATCAATCCTACCAAGTCACTTTACGTTTTGTACAATGAAATCTACTGA", "aa": "MNEFLNEAKAGAKAWLVKAGTSLAVILLVVIGARVYSSSKSAESVIDNPTEEAITFKLDGKDYTLEPKTSQVIKLSKGEHTLEYLGETTKFTKKAPKFLDTDYSIINPTKSLYVLYNEIY", "aa_hexdigest": "61ce9ca8a87227e4c5b05560f75df4e0", "start_type": "ATG", "rbs_motif": "TTCTT", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 520379, "stop": 521020, "observations": { "mutation": [ 520660 ], "stop": [ 520660 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EKR0", "query_cov": 1.0, "subject_cov": 0.5633802816901409, "identity": 0.983, "score": 227.0, "evalue": 5.43e-74, "gene_start": 1, "gene_end": 120, "reference_start": 1, "reference_end": 120, "reference_sequence": "MNEFLNEAKAGAKAWLVKAGTSLAVILLVVIGARVYSSSKSAESVIDNPTEEAITFKLDGKDYTLEPKTSQVIKLSKGEHSLEYLGETTKFTKKAPKFLDTDYSVINPTKSLYVLYNEIYGENLTETEAHEKSSTYDCEDDQGKPDKCPRKFLSDAFIQESVDYGLDEDTPENVDVAKSTRYTIKKKLFRKDDFSRYMDGGNEDVILEPVAVE" }, "paralog": false, "identity": 0.9295774647887324, "score": 394.0, "evalue": 4.02e-142, "description": "Internal stop codon at 520660. Nonsense mutation around 520660.", "psc": { "uniref90_id": "UniRef90_A0A328EKR0", "product": "PEGA domain-containing protein", "uniref50_id": "UniRef50_A0A328EKR0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EKR0", "UniRef:UniRef50_A0A328EKR0" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EKR0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EKR0" ], "product": "PEGA domain-containing protein" } }, "id": "NKOCPLAKPK_990", "locus": "GCA003260355_00954" }, { "type": "cds", "sequence": "contig_1", "start": 521397, "stop": 522110, "strand": "+", "frame": 3, "gene": null, "product": "SIMPL domain-containing protein", "db_xrefs": [ "KEGG:K09807", "SO:0001217", "UniRef:UniRef50_A0A328EH29", "UniRef:UniRef90_A0A328EH29" ], "nt": "ATGAGAGCTCCTGCACCAGTCATTTCTCAGGGAGAACTCCAGCAAAACGTCAACGCACTGAGCGTGCAAGGGAAAGGAGAGGTATATGCTGCTCCTGATATGGCAACCCTCAGCATTTCCCTTTCAAAAACTGCCCCAACAACGAAAGAAGCACAGAATTTCGTTGCGACTAAGATGAGTGAGGTGCAATCACTGCTCAAGAACTTCCCGATTAAAGCCGAAGATATTCAGACGCAGAATATTACGATCGGGACGGAGTATGATTATGGGTTTGGAGGAGGAGAAAGGAAGGTAAAGGGCTATACTGCCACCTACGCCATGGCTCTCACTCTTCGCGAGCTCGATGCCAAGAAGGCAAAAGTGCTAGAAGATCTCATCAACACCCTCTCTGGTATTGAAGGAGTGGGTATTGAAAGCCTCAGCTATGATATTAGAGATAAAACTGAGTTATTTCGTCAGGCGAGATCGCTTGCCATGGAAAAAGCAAAACAGAAAGCTAAGGAACTCGCAAAGCTTGGAGGCGTTCGTATCGGCAAGCCTATAGCGCTCTCTGAAGAGGTTGATGCTACGCCATTTTTTGCTAGAAAGGCGATGGCTTCTCAGTCTAATGTAGCTTTTGCTGATGAAGCGTCCGCGGAAGAGGGAACTTCAACGATTGCGCTTGGACAGCTCAAGGTTTCGGTGGCAATCAATGTAAGCTACCCTCTTATGTAG", "aa": "MRAPAPVISQGELQQNVNALSVQGKGEVYAAPDMATLSISLSKTAPTTKEAQNFVATKMSEVQSLLKNFPIKAEDIQTQNITIGTEYDYGFGGGERKVKGYTATYAMALTLRELDAKKAKVLEDLINTLSGIEGVGIESLSYDIRDKTELFRQARSLAMEKAKQKAKELAKLGGVRIGKPIALSEEVDATPFFARKAMASQSNVAFADEASAEEGTSTIALGQLKVSVAINVSYPLM", "aa_hexdigest": "271c51355841bff986f61065e3c4c429", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A328EH29", "query_cov": 1.0, "subject_cov": 0.8843283582089553, "identity": 0.992, "score": 434.0, "evalue": 4.93e-153, "valid": true, "product": "SIMPL domain-containing protein", "uniref50_id": "UniRef50_A0A328EH29", "kegg_orthology_id": "K09807" }, "pscc": { "uniref50_id": "UniRef50_A0A328EH29", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EH29" ], "product": "SIMPL domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_991", "locus": "GCA003260355_00955" }, { "type": "cds", "sequence": "contig_1", "start": 522656, "stop": 523057, "strand": "+", "frame": 2, "gene": null, "product": "Tetratricopeptide repeat protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A948C424" ], "nt": "TTGGGGCTCTACATTAAGGGGCTTTTGGAAATGGAGAAGAATAATCGGAAAGAAGCAAGAAAATATCTCTCAAAAGCGATGGAAATGACCAATGGAACGAATCATGAGATCCTAAGATGTTATGGGCTTTGTGAGTATTGGTATGGGAACAGGTCGAAAGGGCTCGAGTATCTGAAAGATGCTTTCGCCTTGGACTGCAAAGATGCTGAAGTGATTTACAATCTGATCCAGATGTACATTCTCGAGCAAGAATACAAGAAAGCTCAGGAAATGATTAGCTACTTCAACAAGCATCAAGCCTCGCTCAAGTTTGTGGACAAGAAGCTTGACTTCTATCTAACGAAGATCTCTTTGTTTGAGAAGTTTATCAAGGCGAAGAAGCTTTTCCAACTGAGAAAATAA", "aa": "MGLYIKGLLEMEKNNRKEARKYLSKAMEMTNGTNHEILRCYGLCEYWYGNRSKGLEYLKDAFALDCKDAEVIYNLIQMYILEQEYKKAQEMISYFNKHQASLKFVDKKLDFYLTKISLFEKFIKAKKLFQLRK", "aa_hexdigest": "c10dad07885a696da1a1e65378008902", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A948C424", "query_cov": 0.9323308270676691, "subject_cov": 0.9465648854961832, "identity": 0.597, "score": 152.0, "evalue": 2.18e-45, "valid": false, "product": "Tetratricopeptide repeat protein", "uniref50_id": "UniRef50_A0A948C424" }, "pscc": { "uniref50_id": "UniRef50_A0A948C424", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A948C424" ], "product": "Tetratricopeptide repeat protein" }, "genes": [], "id": "NKOCPLAKPK_992", "locus": "GCA003260355_00956" }, { "type": "cds", "sequence": "contig_1", "start": 523094, "stop": 524026, "strand": "+", "frame": 2, "gene": "ftsX", "product": "FtsX extracellular domain-containing protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBFA1E7", "UniRef:UniRef100_A0A328EGR7", "UniRef:UniRef50_A0A839JTQ4", "UniRef:UniRef90_A0A328EGR7" ], "nt": "ATGAAAACGAAACACTTTGGATTAAATCTTATTAAATCCGACACGAAGACGTTGCTCAAGTCGTATCGCATTCCATTGATTCTGATTAGCATCCTGATGGGAGTATTACTCTTTGTCATGAATGTTTTCCTCGCTGGAGCGCTCTACGGGAATAGTTTTAATCTCCAGATGAAAGAGAAACTTGGGGTTTATCTCTATCTCAGCGATGCTGCTACTCAAGATCATACTGATGCTGCGCTTAAGTCTGCATTGGAAGAGAAAGGATTGAAAGTAGATTATGCCTCTAAGCAAGAATCGCTTGAGTTTGTAGAGAAAAGGATCCCTGATCTAACAAGCACCCTCAGGAAATACAACCTTGAGAATCCGCTACCTGCAACGCTCTATGTAAGGTATGGAGATCAGAATGAGTTTGCTGCGATGAAAGGAGTCCTTGAAAACTACAAGACTCAAATCCTGAATATGGACGATCTCTCTGACAATGCGATCAAAACTCAAGAAAAAAGAGTGCTGAACGTGATCAATCTCTCTAATTTTTTGCAGAGTTTTGCGTATTTTGTGGTGCTAGCGCTTGTGCTTACGATTATTGCTTTTGCGATTTTCTTCTTGAAGACGATCTTTTCACATTTTATAGAGGATATTCAGGCAAAGAAGCTCTTGGGTGCTACGTCTCGTCAGATTGTGCAACCGTTTCTGAATGTGATCTTTATGAGCTTGAGTTTTGCGTTCGTGCTTGCGATTGTTCTCCTCGTAGGTGGATTGATTCCGCTTGATTCCTATCTCTCTGCTCTCTTTGGGTTTGATCTCTTTGGGTATTTGTCAGGGTTAGCGATTGATTTGCTCGCGGTTGGAGTAATCGAGATTGGAGGAATTATGTTGCTCCTCCTCTGCATCTCTTACCGTTTCGTAATGAGGCTTCATAGGAAGTTGAAGTAA", "aa": "MKTKHFGLNLIKSDTKTLLKSYRIPLILISILMGVLLFVMNVFLAGALYGNSFNLQMKEKLGVYLYLSDAATQDHTDAALKSALEEKGLKVDYASKQESLEFVEKRIPDLTSTLRKYNLENPLPATLYVRYGDQNEFAAMKGVLENYKTQILNMDDLSDNAIKTQEKRVLNVINLSNFLQSFAYFVVLALVLTIIAFAIFFLKTIFSHFIEDIQAKKLLGATSRQIVQPFLNVIFMSLSFAFVLAIVLLVGGLIPLDSYLSALFGFDLFGYLSGLAIDLLAVGVIEIGGIMLLLLCISYRFVMRLHRKLK", "aa_hexdigest": "7960078751a82861317f23faefd53725", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000DBFA1E7", "uniref100_id": "UniRef100_A0A328EGR7", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBFA1E7", "UniRef:UniRef100_A0A328EGR7" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EGR7", "uniref90_id": "UniRef90_A0A328EGR7" }, "psc": { "uniref90_id": "UniRef90_A0A328EGR7", "product": "FtsX extracellular domain-containing protein", "uniref50_id": "UniRef50_A0A839JTQ4" }, "pscc": { "uniref50_id": "UniRef50_A0A839JTQ4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A839JTQ4" ], "product": "FtsX extracellular domain-containing protein" }, "genes": [ "ftsX" ], "id": "NKOCPLAKPK_993", "locus": "GCA003260355_00957" }, { "type": "cds", "sequence": "contig_1", "start": 524432, "stop": 524833, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAACGAATTACCAAGCTTTTTCTCTGGATGATGAAAGCGCGCTACACGATTAGTGTGAAGCATTTTCCAAAGCTCGATCCAGAATACAACTATTTCATTCTGCCGAATCATATCGCGTATTTGGATCCTGTGCTATTGTGGGCGATGTTTTATCCGGAGAGGAAGCTGAGACCGGTAGCAACTTCGAGGTTTGCGGAGAATTTCTTTTTGAGGTGGCTATTTCGCTGGATTGGGACGATCAGCATCGTCGAGATGGCAAGAGAGAAGAAAGGATTAGCACCGAGAGAAGAGAAGCTCAACTCAGCCATGGAAGCTTTACAAGAAGCGTTGGCGCAGGGGGATTCGGTATTGCTGTATCCGTCTGGGCAACTAGCGGGGCAAGGGTTTGAATCGCTGTGA", "aa": "MQRITKLFLWMMKARYTISVKHFPKLDPEYNYFILPNHIAYLDPVLLWAMFYPERKLRPVATSRFAENFFLRWLFRWIGTISIVEMAREKKGLAPREEKLNSAMEALQEALAQGDSVLLYPSGQLAGQGFESL", "aa_hexdigest": "9bd0510248d8ad31ad6fb9e6b7821b40", "start_type": "ATG", "rbs_motif": "TTTA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15603.137099999978, "isoelectric_point": 9.432063102722168 }, "id": "NKOCPLAKPK_994", "locus": "GCA003260355_00958" }, { "type": "cds", "sequence": "contig_1", "start": 524858, "stop": 526705, "strand": "+", "frame": 2, "gene": null, "product": "Carrier domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EGE9", "UniRef:UniRef90_A0A328EGE9" ], "nt": "GTGCAAGGATTAGGAAAAAAGACAAGAGTTTTGCTTGTAAGAACCAGAGGGCTCTGGGGAAGTATTTGGTCGAACGCTTGGACAGGAGAGACACCGAGTTTGGTCGGGGGGATTGTGAAAGGGCTTTTCTATCTGCTAGCAAATGGGTTTGTTTTCTCACCAAGGAGGAAGGTTGAGCTGGAGTTTTGTGATATGACGGAAGAAGTCAAACAAGCGAGCAAAGAAGGATTAGAGGTTTTCAATCAGACTTTAGAAGCGTTTTATAACGGGAAAGGGGAAGAGAAGGTAAAATACATTCCTCACTTTTTCTACTACAACGATGTTGAAGGGAAAGTGTTGCCTGCACACGTGAGAAATTCTGTAGAGGATCTCCAAGCAGTGAATGGCTATGAAGGATTTGAGTTTGCTGAGGAGGTCGTAGCGACGGTTTGTGCCGAGCTAAGGAGGATTAGGGAGTTACCTCAGCAAGCAGAGATTACACTAGAAAGTAATCTGATTTTGGATCTGTATTTAGATTCGCTTGATATGGCGGAGATCAAGAATTTCATCCTGACTCGCTATGCAGGAGCATCAAACACTCCCCTTCTAGAACTGAAAACGGTTGCGGATTTGGTAGCGATGGCGATGGGGAAAACCGCAAATGAAAAATCTGATTTCCCTCCTTGTGAATGGGAGCTAGAGAACAAGGAGTATTGCGAGCCTCAGAAGGAGTTTGATGAAGATATGACGATTTTGGAGCTGTTTAGGAAGCAATGGAAAGCTGATCCGGATGCAACGCAGAGCTATGATGTGATGTATGGGATGCAGAAGAGGAAAGACATTGTATTGAAATCGTTTTTGATTAGCGACTATCTGAAAGCTATCCCTGGGAAGCAGATTGGGATTATGTTGCCAGCGGTCGGAAGTATGAATGTATTGCTCTTGGCAGCATATTTGGCTGAAAAAGTGCCGGTAATGATGAACTGGACGCATCCTCAAAGTGCGTTTGCACACTGTGTCAAGTTTTCAAAGACAAAAAAGATTCTGACTTCAAAAGCGTTTTTCAACAAGCTCAATATTGAACGGCTGAAAGAGTATGAGTTTGTCTTTTTGGAGGATTTATTGAAGAAGATTCCGCTTTATCGCAAGCTCAAGGCTTTGGCGAAGAGCTGGGTATTCCCATTGCCTCCTCAGTCTAGTGAAGATCCTGCGGTCGTGCTTTATACCTCAGGAAGTGAGGCACTACCGAAAGCGGTGCCACTCACCCACAAGAATCTGATTTCTAATCTGATAGGAGCATTGAGCATTATGCATATCAAGCAAGATGAAAGATTTTTCTGTTATTTACCACCGTTTCATAGTTTTGGGTTTACGGTAAATACAGTGTTTCCCTTGGTAGCGGGGTTTAGGAGTGTTAGCACGCCTGATCCTAACGATTCGCTGACGGTTGCCCAATTGATTGCTCATAGCAAACCTACTTTGCTTGCGACGACTCCGACCTTCTTGAGGAATCTTTTGAATGTTGCGAAAAATGAGCAGCTTGCATCACTGCGTTATGTGATTACGGGAGGAGAGAAATGTTCGGAGAGTTTATTTGAGAGATTTAGGCAAGCGGTGCCTCATGGGGTGATTCTTGAAGGCTATGGGATTACTGAGTGTAGCCCGATCATTGCGATCAATCCGATTGAAAAGCAGAAAGCTCAATCGGTAGGGATTGCTGTAGGAAATGGTGAGCTTTGCATTCTGGATCTCAACACGCAAGAAGTATTGAAGCAGAATCAAGAAGGGATGATTTATTACTGCGGTCCGAATGTGTTCTCCGGCTATCCCGATCAGAGCATTGAAAGCCCATTCTGGGAGCAGGATTGA", "aa": "MQGLGKKTRVLLVRTRGLWGSIWSNAWTGETPSLVGGIVKGLFYLLANGFVFSPRRKVELEFCDMTEEVKQASKEGLEVFNQTLEAFYNGKGEEKVKYIPHFFYYNDVEGKVLPAHVRNSVEDLQAVNGYEGFEFAEEVVATVCAELRRIRELPQQAEITLESNLILDLYLDSLDMAEIKNFILTRYAGASNTPLLELKTVADLVAMAMGKTANEKSDFPPCEWELENKEYCEPQKEFDEDMTILELFRKQWKADPDATQSYDVMYGMQKRKDIVLKSFLISDYLKAIPGKQIGIMLPAVGSMNVLLLAAYLAEKVPVMMNWTHPQSAFAHCVKFSKTKKILTSKAFFNKLNIERLKEYEFVFLEDLLKKIPLYRKLKALAKSWVFPLPPQSSEDPAVVLYTSGSEALPKAVPLTHKNLISNLIGALSIMHIKQDERFFCYLPPFHSFGFTVNTVFPLVAGFRSVSTPDPNDSLTVAQLIAHSKPTLLATTPTFLRNLLNVAKNEQLASLRYVITGGEKCSESLFERFRQAVPHGVILEGYGITECSPIIAINPIEKQKAQSVGIAVGNGELCILDLNTQEVLKQNQEGMIYYCGPNVFSGYPDQSIESPFWEQD", "aa_hexdigest": "dd27bdf400fedfadaf1fcccb331f15ab", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 524558, "stop": 527008, "observations": { "mutation": [ 524831, 526703, 526796 ], "stop": [ 524831, 526703, 526796 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EGE9", "query_cov": 1.0, "subject_cov": 0.6949152542372882, "identity": 0.977, "score": 1191.0, "evalue": 0.0, "gene_start": 1, "gene_end": 615, "reference_start": 143, "reference_end": 757, "reference_sequence": "MQRITKLFLWMMKARYTISVKHFPKLDPEYNYFILPNHIAYLDPVLLWAMFYPERKLRPVATSRFAENFFLRWLFRWIGTISIVEMAREKKGLAPREEKLNSAMEALQEALAQGDSVLLYPSGQLAGQGFESLGGKRAAYQTVQGLGKKTRVLLVRTRGLWGSIWSNAWTGETPSLVGGIVKGLFYLLANGFVFSPRRKVELEFCDMTEEVKQASKEGLEVFNQTLEAFYNEKGEEKVKYIPHFFYYNDVEGKVLPAHVRNSIEDLQAVNGYEGFEFAEEVVATVCAELRRIRDLSQQAEITLESNLILDLYLDSLDMAEIKNFILTRYAGASNTPLLELKTVADLVAMAMGKTANEKSDFPPCEWRLENKEYGEPQKEFDEDMTILELFRKQWKADPNATQSYDVMYGMQKRKDIVLKAFLISDYLKAIPGKQIGIMLPAVGSMNVLLLAVYLAEKVPVMMNWTHPQSAFAHCVKFSKTKKILTSKAFFNKLNIERLKEYEFIFLEDLLKKIPLYRKLKALAKSWVFPLPPQSSEDPAVVLYTSGSEALPKAVPLTHKNLISNLIGALSIMHIKQDERFFCYLPPFHSFGFTVNTVFPLVAGFRSVSTPDPNDSLTVAQLIAHSKPTLLATTPTFLRNLLNVAKSEQLASLRYVITGGEKCSESLFERFRQAVPYGVILEGYGITECSPIIAINPIEKQKAQSVGIAVGNGELCILDLSTQEVLKQNQEGMIYYCGPNVFSGYPDQSIESPFWEQDGKVWYRTGDLGYRDKDGYLYITGRKKRFLKLGGEMISLPFLEGLLLEQRGNSEEANLALEGKEHEHGIEITLFVVDLKISLKEVNSYLRAKGVSNLIQVNHIKKIGAIPLLGTGKIDYKVLKGMIDGH" }, "paralog": false, "identity": 0.9791666666666666, "score": 1595.0, "evalue": 0.0, "description": "Internal stop codon at 524831, 526703, 526796. Nonsense mutation around 524831, 526703, 526796.", "psc": { "uniref90_id": "UniRef90_A0A328EGE9", "product": "Carrier domain-containing protein", "uniref50_id": "UniRef50_A0A328EGE9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EGE9", "UniRef:UniRef50_A0A328EGE9" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EGE9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EGE9" ], "product": "Carrier domain-containing protein" } }, "id": "NKOCPLAKPK_995", "locus": "GCA003260355_00959" }, { "type": "cds", "sequence": "contig_1", "start": 526805, "stop": 527038, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTTCTCTGCCGTTTCTAGAAGGACTTCTCTTGGAGCAACGAGGGAATAGCGAAGAAGCGAATCTTGCACTTGAGGGGAAAGAGCATGAACATGGGATTGAAATAACGCTCTTTGTGGTTGATCTGAAGATTAGCTTGAAAGAAGTAAATAGCTATCTGAGATCGAAAGGGGTTTCCAATCTGATTCAGGTTAATCATATCAAGAAAATTGGTGCGATTCCACTCCTCTGA", "aa": "MISLPFLEGLLLEQRGNSEEANLALEGKEHEHGIEITLFVVDLKISLKEVNSYLRSKGVSNLIQVNHIKKIGAIPLL", "aa_hexdigest": "e36108f7c9a2fb8fe355169eb45d167c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8561.901799999992, "isoelectric_point": 5.8581335067749025 }, "id": "NKOCPLAKPK_996", "locus": "GCA003260355_00960" }, { "type": "cds", "sequence": "contig_1", "start": 527373, "stop": 527969, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGGGATTAACTGCACTAATGAGTCTCCCAAAAGAGTCCTACCCTGAAGTTCAACTCGGAATGGTTTCTATTGCGACGGTGTATCCTGGAGGGAATCCTCGTGAGATTGATCAGCTTATCACACAGAAGATTGAGAAGAAGATCAAGAATATGGATGGAGTGAAGAAGCTTACCTCGACCTCTCGCAAGAATGTTTCTTCTATCCTCGTCGAACTAACAGACAAAGTCAAGGCAACCGATATGAGTTCCAAGATTAAAGATGCAGTGAGTCAGCTTTCTTTTCCTGAGAATGTGGAATCTCCGACGGTTACTGAGATTGACACTCCTAAAGATAGGCTTTTTACGCTGCTCCTCTATGCTGAAAATCCTGCGATGGATCAGCACTACCTCAAGACCAAAGCTAATCAACTGAAAGCAGAACTGGAAGGAAAAGGTGCTATTGATACCATAGAGATCGAAGGAGGAAATAAGTATACTCTTGAGCTTCTGGTAGATAAACAGAGAATAGAAAGTCTTGGGCTTTCACTTACTCAGATTGCTGGGCAGCTTCAATCCTTTACAAGCAATCAACCGCTAGGAAATCATCAACTCTGA", "aa": "MLGLTALMSLPKESYPEVQLGMVSIATVYPGGNPREIDQLITQKIEKKIKNMDGVKKLTSTSRKNVSSILVELTDKVKATDMSSKIKDAVSQLSFPENVESPTVTEIDTPKDRLFTLLLYAENPAMDQHYLKTKANQLKAELEGKGAIDTIEIEGGNKYTLELLVDKQRIESLGLSLTQIAGQLQSFTSNQPLGNHQL", "aa_hexdigest": "d0c894c49ff6ae47fd7cd890a8b2be85", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 21832.8496, "isoelectric_point": 5.7886762619018555 }, "id": "NKOCPLAKPK_997", "locus": "GCA003260355_00961" }, { "type": "cds", "sequence": "contig_1", "start": 527973, "stop": 528569, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACTATGATTTTAGGATTGATGGAGAACTTAAGTCTGAGGAAGAACTGCAGGCATTGCCGATTTTATTGGGTGGAGGGAAGAGTATTCCGCTTGGTCAGCTCGCGGACATCAAGAAAGTCTATGACGATAAAGATAGTATTCTCCGTGTCGGGACCAAAGAGAAAGGGATTGGAAAAGCTGCAGTTAGCCTAACGTTTAATAAAAAGGCGGGAGCCAGCATTCTCTCTAGTAGTAAGCAGGCGAAAGAAAGTATCGCAGCAGCTCTTAAAGATGCTGAATATGCTGAGCTGAGCTCTTTTTATACCTTGGATCTTGGTGCAGAACTCTCCAAAGACTACAACAATCTAGCAGTCAATATGCTTAGCACGATGCTGATCGTCTTTTTGATTGTGGGATTTTTTGTCGGGGCGATGGAGTCTTTTGTGGCTACGGTTTCTATTCCTTTAGCATTTTTCGTGACGTTCTTTGTGCTGAACTATCTCGGATTGAGTCTGAATTTTTTGACTAATTTCTCACTGATTATTTGCCTAGGAATTGCTATTGATACGGCAACGGTAATCATTCAAGGGGCAAGTGAAAATATAAAACTCTGA", "aa": "MNYDFRIDGELKSEEELQALPILLGGGKSIPLGQLADIKKVYDDKDSILRVGTKEKGIGKAAVSLTFNKKAGASILSSSKQAKESIAAALKDAEYAELSSFYTLDLGAELSKDYNNLAVNMLSTMLIVFLIVGFFVGAMESFVATVSIPLAFFVTFFVLNYLGLSLNFLTNFSLIICLGIAIDTATVIIQGASENIKL", "aa_hexdigest": "05656445bfca8b58b6b42e1789bf6803", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 21324.586100000004, "isoelectric_point": 4.920688056945799 }, "id": "NKOCPLAKPK_998", "locus": "GCA003260355_00962" }, { "type": "cds", "sequence": "contig_1", "start": 528579, "stop": 529478, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCATGCGGCGTTGCTCTCTGTGAGAACGTATAAAAATTCGCTGATTTCTGGGACAGCTACAACGGTTATTGTTTTCGTTCCGATGCTGACTTTGCCAGGGATTATGGGGAAGTTTTTGTCCTTTATCCCGATCACGATCTTTATTACGCTTCTGGCTTCACTCTTTATCTCGCTTACGCTGACACCAGCACTTTTTTCAAAGTTTACTAAGAACGGGACGAACTACACCTCAAGCTCAGAGGATGAAAATCTCCTCAATCCTGAAGAACAAGCGCTGCTTGCAGACGACAGATCAGACAAAACTCAGCTTGAGAACGCTCAAGCCTCTGGGGTTCGTGAACGCATCTTTTCTGCGATGATCTCGCGATATGAGAGGACGCTGACTCCCCTCTTGAAAAGTGCTAAGACGAGGATTACTCTCATTCTAATTCCGATTATTCTCCTGATCATCACGGTTGTGTTTGTGAGTCCGAGAGTAGGGTTTTTGATGATGCCCAAAACGGATAACGAGTATCTTACGATTGTTTTGTCTGCAAAGGAAGGGACTACTACAGAGACGCTCTATGCAAAGACCCAACAAACTGAATCCATCATTGGGAAGCTTCCTGAGCTAAGTAACTACAGTTGGAAGCTAACAGATAATACGGCAACTTTGAGCATCCGCATTGTGCCAAAAGAAGAGAGAGAAAGAGACAGCTTTGCTATTGAAAAGGAGATTGAAGAGCAGCTTGCATTCTTGAAGCAAGAGGGAATTACGGTGGAATCCAAGGTGCAAAGTAATGGTCCAAGTGATGGTGCTGAGATTGGTATCAAACTTACTGCCAGTAGTATTGATGAGCTTACTGAACTCATACGTGTAGTTGAGGACTTCAAAACTTTTCTCAAAGGAGTGCCGTGA", "aa": "MHAALLSVRTYKNSLISGTATTVIVFVPMLTLPGIMGKFLSFIPITIFITLLASLFISLTLTPALFSKFTKNGTNYTSSSEDENLLNPEEQALLADDRSDKTQLENAQASGVRERIFSAMISRYERTLTPLLKSAKTRITLILIPIILLIITVVFVSPRVGFLMMPKTDNEYLTIVLSAKEGTTTETLYAKTQQTESIIGKLPELSNYSWKLTDNTATLSIRIVPKEERERDSFAIEKEIEEQLAFLKQEGITVESKVQSNGPSDGAEIGIKLTASSIDELTELIRVVEDFKTFLKGVP", "aa_hexdigest": "2c780920a57dc4782e2c8edfee99d579", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 33161.03630000005, "isoelectric_point": 5.36062183380127 }, "id": "NKOCPLAKPK_999", "locus": "GCA003260355_00963" }, { "type": "cds", "sequence": "contig_1", "start": 529554, "stop": 530138, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTGAATTTGACGCCAAGGAATTTTGCACCCGAACTTTATTTTTCACTAAATGGTATCAAAGCTGGCTCTTTCACGGATAACGTTGAAACCCATGACCTCAAGATTAAATATGCAGACTTTACAAAAGAGGTCAGTCCTGACAACCTCATGGGAAGCTTTTTCTCGACCCAACAAGGGAAGGTTGCCCTCGCGAGTGTCGGTTCCTATAGTTTTGCTCCTGCGATTAGCAAGATTTCAAGGGATAACGGCACGATCACGATTTCTGTGCAATCAAATCTTGAGCAAGGGGCAAAGGCTGAAAACGTAAATGCTGCTCTCACACAATTTGCACAATCCTACAAATTCCCCAAAGGAGTCACTTATGCAAAAGGAGGAGAAACAGAAGATAATGCTGATCTCTTGAATGCGATGTTTATGGCGTTTTTGATTTCAATTATCGGGATTTTCGCTATTTTGGTGCTGCAGTTTGATAGCTATACTCAGCCGGGGATTATCCTCTATAGTGTGGTTATGGGATTTTTGGGAGCAACGTATGGACTCTGGATCGTTGGGATTCCCTATGGAATGATGTTCTTGATCTGA", "aa": "MLNLTPRNFAPELYFSLNGIKAGSFTDNVETHDLKIKYADFTKEVSPDNLMGSFFSTQQGKVALASVGSYSFAPAISKISRDNGTITISVQSNLEQGAKAENVNAALTQFAQSYKFPKGVTYAKGGETEDNADLLNAMFMAFLISIIGIFAILVLQFDSYTQPGIILYSVVMGFLGATYGLWIVGIPYGMMFLI", "aa_hexdigest": "ce71af49089e5da15867970f1e358d58", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 21108.019799999987, "isoelectric_point": 5.060682487487792 }, "id": "NKOCPLAKPK_1000", "locus": "GCA003260355_00964" }, { "type": "cds", "sequence": "contig_1", "start": 530301, "stop": 530630, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTACCCTGACAGGAGACGGAATGTTTGCTCCGCTTGCATGGACGATTATCTTTGGATTGAGCGTGGCAACGATGATGACGCTATTTGTCATCCCAGCACTCTATGAAGATGAAGGAAAAATTAGATACCTCATTGCGAGAGTGATTCTGAAACCATTGGTAAAACTGATGCTCCCTACTACAGGACTCGGTATCGTATGGGGACTTTCCACGATGTTTGGAACTCCACTCTTTACAGGAGCCTTTGCTATGCCGTTTTCGTTAGCATTTTTGATCGCGGGATTTTTAATGCTCAACCGATACGGAGTGCAGTGCAATCTTCAGTGA", "aa": "MVTLTGDGMFAPLAWTIIFGLSVATMMTLFVIPALYEDEGKIRYLIARVILKPLVKLMLPTTGLGIVWGLSTMFGTPLFTGAFAMPFSLAFLIAGFLMLNRYGVQCNLQ", "aa_hexdigest": "c5ec9a7d4a7cf9ec36a2c4729f78bc9d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11941.412299999989, "isoelectric_point": 8.977689933776855 }, "id": "NKOCPLAKPK_1001", "locus": "GCA003260355_00965" }, { "type": "cds", "sequence": "contig_1", "start": 530655, "stop": 530810, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGCTCCAACTTACTAATCAGCAAGGCACTGCTTTAAATAAAAAACAGCTGATAAAAAGAGTTTTCTTTAAGTGGGGGCTTTTGATTATGCCGGCACTCTTGGGAGCGGTTCTTTCTGGAGTGGCACAAGCAACGGGAATTGAAATGCTTTGA", "aa": "MKLQLTNQQGTALNKKQLIKRVFFKWGLLIMPALLGAVLSGVAQATGIEML", "aa_hexdigest": "69c0432362c9e77242150d80c8e8df09", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5583.765200000001, "isoelectric_point": 10.582501411437985 }, "id": "NKOCPLAKPK_1002", "locus": "GCA003260355_00966" }, { "type": "cds", "sequence": "contig_1", "start": 530986, "stop": 531564, "strand": "-", "frame": 1, "gene": null, "product": "YgjP-like metallopeptidase domain-containing protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBFBC54", "UniRef:UniRef100_A0A328EGZ8", "UniRef:UniRef50_A0A2W4KFZ4", "UniRef:UniRef90_A0A328EGZ8" ], "nt": "ATGAAAATCATCTACAAAAAGGGTATTCGCAACGGCTATGCCAAGCTTGAAGGGGAGAGCGTCTGCCTGATTATTCCGTCTATTATGCAGGGCGATCAAGCCTTCCTCCAGAAAATGCAAACTCTAGGAGAAAAGCTTCAGCAAAAAGCCGACCAAAAGCAAAAAAATCAGATTTTTTCTCCTGAGGGCGTTCTGCTCTTTGGCGAGAGAATCCCGCTAACGGACCTCCCCCCGATCAAGGACCAAAAACAGCGAACTGCCTTCTTCAAACAAGAACTCTTTGACTACGCAAATCCGCTCCTGCAACGCTTTGCCAGTGAGCTTGGCTTCCCGCCTATTCCGCTCACGATCAAAAAAGTGCAGTCCAAACGGGGATCCTGCACCTATGATAACCGCATTATGCTCAATCTTTCCCTCGTGCATCTTCCGACGAGGCTCACGCAATATGTTGTTGCCCATGAGGCGTGCCACCTGCTAGCCAAGCACCATCAGACGAGTTTCCGAGCTCTTGTAGCCAAGTTTTGTCCTCATTATAAAGCATTGAGGAAAGAGTTAAAAAATCAGATTTTTATTTCCTAG", "aa": "MKIIYKKGIRNGYAKLEGESVCLIIPSIMQGDQAFLQKMQTLGEKLQQKADQKQKNQIFSPEGVLLFGERIPLTDLPPIKDQKQRTAFFKQELFDYANPLLQRFASELGFPPIPLTIKKVQSKRGSCTYDNRIMLNLSLVHLPTRLTQYVVAHEACHLLAKHHQTSFRALVAKFCPHYKALRKELKNQIFIS", "aa_hexdigest": "cb02f3f09707d83823b8b4b51067a00e", "start_type": "ATG", "rbs_motif": "TTATT", "ups": { "uniparc_id": "UPI000DBFBC54", "uniref100_id": "UniRef100_A0A328EGZ8", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBFBC54", "UniRef:UniRef100_A0A328EGZ8" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EGZ8", "uniref90_id": "UniRef90_A0A328EGZ8" }, "psc": { "uniref90_id": "UniRef90_A0A328EGZ8", "product": "YgjP-like metallopeptidase domain-containing protein", "uniref50_id": "UniRef50_A0A2W4KFZ4" }, "pscc": { "uniref50_id": "UniRef50_A0A2W4KFZ4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4KFZ4" ], "product": "YgjP-like metallopeptidase domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_1003", "locus": "GCA003260355_00967" }, { "type": "cds", "sequence": "contig_1", "start": 531680, "stop": 532078, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBFDFE6", "UniRef:UniRef100_A0A328EJZ5", "UniRef:UniRef50_A0A328EJZ5", "UniRef:UniRef90_A0A328EJZ5" ], "nt": "ATGACAGATTTGAAGCTTCTGAATTGTCATGTTTCGCTCGCAATGACGGTTTTGAAGCCGCTGGATTGCTACGCTCCGCTCGCAATAACAGGTTTGAAGTCTCTGGATTCCCATGCTTCACTCGCAATGACAGATTTGAAGCTTCTGAATTGCCACGCTTTGCTCGTAATGACGGGTTTGAAGCTTCTGGATTGCCACGCTTTGCTCGTAATGACGGGTTTGAAGCTTCTGGATTGCCACGCTTCGCTCGCAATGACAGGTTTGAAGTCTCTGGATTCCCATGCTTCACTCGCAATGACAGGTTTGAGGTCTCTGGATTACTTCACTTCGTTCGCAATGACGGGTTTGAAGTCTCTAGATTGCTTCACTTTGTTCGCAATGACGGCGGGAGAGGATTAG", "aa": "MTDLKLLNCHVSLAMTVLKPLDCYAPLAITGLKSLDSHASLAMTDLKLLNCHALLVMTGLKLLDCHALLVMTGLKLLDCHASLAMTGLKSLDSHASLAMTGLRSLDYFTSFAMTGLKSLDCFTLFAMTAGED", "aa_hexdigest": "5b86c323fcb66af3a28c17323fdd6101", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000DBFDFE6", "uniref100_id": "UniRef100_A0A328EJZ5", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBFDFE6", "UniRef:UniRef100_A0A328EJZ5" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EJZ5", "uniref90_id": "UniRef90_A0A328EJZ5" }, "psc": { "uniref90_id": "UniRef90_A0A328EJZ5", "uniref50_id": "UniRef50_A0A328EJZ5" }, "pscc": { "uniref50_id": "UniRef50_A0A328EJZ5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EJZ5" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14135.77339999998, "isoelectric_point": 6.258393669128417 }, "id": "NKOCPLAKPK_1004", "locus": "GCA003260355_00968" }, { "type": "cds", "sequence": "contig_1", "start": 532075, "stop": 532464, "strand": "-", "frame": 1, "gene": "smpB", "product": "SsrA-binding protein SmpB", "db_xrefs": [ "GO:0003723", "KEGG:K03664", "SO:0001217", "UniRef:UniRef50_A0A328EK58", "UniRef:UniRef90_A0A328EK58" ], "nt": "GTGCTCAAAGGTCATGAAGTGAAATCAATCAAGACTTCGCATATCAATATCCAGGATGCAGTAGTGAGGATCGATAATCATGAATGCCGACTCTATAATATGGATATTCCGCTCTATGAAAAGACCTCTCCCATTCTTGTTCCGTATTACCAAGCGAAAGCAAAGAGGAAGCTCCTTCTCAACAAGAGAGAAATCACGAGAATTGCTTCAGCGCTTGATAAGCCAGGGATGGTGCTCCTTGCATTAGAAGTCATAGTTACCAAAGGTGGTTTCATCAAAATCAAACTCGGGCTAGGGAAGCTTTACAAAAAAGTAGAAAAAAAGCAGATCCTCAAGGAAAAGGACATCAAAAAACAAATGGATAGAGAGGTGAGTGCCTACAAATACTAA", "aa": "MLKGHEVKSIKTSHINIQDAVVRIDNHECRLYNMDIPLYEKTSPILVPYYQAKAKRKLLLNKREITRIASALDKPGMVLLALEVIVTKGGFIKIKLGLGKLYKKVEKKQILKEKDIKKQMDREVSAYKY", "aa_hexdigest": "48590e9663560a6e23997edcd9b00f1c", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A328EK58", "query_cov": 1.0, "subject_cov": 0.8431372549019608, "identity": 0.961, "score": 238.0, "evalue": 5.84e-79, "valid": true, "gene": "smpB", "product": "SsrA-binding protein SmpB", "uniref50_id": "UniRef50_A0A328EK58", "kegg_orthology_id": "K03664", "go_ids": [ "GO:0003723" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EK58", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EK58" ], "product": "SsrA-binding protein" }, "genes": [ "smpB" ], "id": "NKOCPLAKPK_1005", "locus": "GCA003260355_00969" }, { "type": "cds", "sequence": "contig_1", "start": 532682, "stop": 533050, "strand": "+", "frame": 2, "gene": null, "product": "Class I SAM-dependent methyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MVG4", "UniRef:UniRef90_A0A660MVG4" ], "nt": "ATGAAGATCATAGATGAACTAGAGCAATTTTATACTGCTGAGGCTCAGAAATACGCCCAGACGAGAAGAAAGCATCGACCTGATGGAGAACGCTTATTGGAAGCATTGGAAAGTTTAGAAATGAGGAGTCCGAAACTTCTCGAGCTTGGATGCGGCAGCGGAAGACTAGTTTCCTATCTTGACCACGCATGGGAAAGGAAGTTTGCCTATACAGGAGTTGATCTTTCCTCAGGGCTATTGAAGTATGCGAAGGAAGCCAATCCTGGACGTAAGTTTATCTGTAGCGAGATGACGAGTTTCCTTGAAGGATGTAAGCAGGAGTCGTTTGATGCAGTGGTAGCGTGTGCGAGCTTTCAGCATATCCCGTGA", "aa": "MKIIDELEQFYTAEAQKYAQTRRKHRPDGERLLEALESLEMRSPKLLELGCGSGRLVSYLDHAWERKFAYTGVDLSSGLLKYAKEANPGRKFICSEMTSFLEGCKQESFDAVVACASFQHIP", "aa_hexdigest": "1ef02a5381b1511fe59bb9c97b507faa", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 532682, "stop": 533353, "observations": { "mutation": [ 533048 ], "stop": [ 533048 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A660MVG4", "query_cov": 1.0, "subject_cov": 0.4899598393574297, "identity": 0.9590000000000001, "score": 239.0, "evalue": 2.58e-78, "gene_start": 1, "gene_end": 122, "reference_start": 1, "reference_end": 122, "reference_sequence": "MKIIDELEQFYTAEAQKYAQTRRKHRPDGERLLEALENLEMKSPKLLELGCGSGRLISYLDQAWERKFAYTGVDLSSGLLKYAKEANPGRKFVCSEMTSFLEGCKQESFDAVVACASFQHIPSAKARLAVMKNAYRALRYEGLLVMTNRAFSEWFLRKHWKVVLQSGLKAVFTLGNADWRDVMIPWKAKKQVYHRYYHLFGLEELEKLAEMAGFWVEECHFLDKGGKKVEDWHKANNSFLVARKVICKG" }, "paralog": false, "identity": 0.9551569506726457, "score": 443.0, "evalue": 7.38e-161, "description": "Internal stop codon at 533048. Nonsense mutation around 533048.", "psc": { "uniref90_id": "UniRef90_A0A660MVG4", "product": "Class I SAM-dependent methyltransferase", "uniref50_id": "UniRef50_A0A660MVG4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A660MVG4", "UniRef:UniRef50_A0A660MVG4" ] }, "pscc": { "uniref50_id": "UniRef50_A0A660MVG4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MVG4" ], "product": "Class I SAM-dependent methyltransferase" } }, "id": "NKOCPLAKPK_1006", "locus": "GCA003260355_00970" }, { "type": "cds", "sequence": "contig_1", "start": 533072, "stop": 533431, "strand": "+", "frame": 2, "gene": null, "product": "Class I SAM-dependent methyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MVG4", "UniRef:UniRef90_A0A660MVG4" ], "nt": "ATGAAGAATGCCTATAGAGCACTCCGTTATGAGGGGCTACTCGTGATGACGAATCGAGCATTTTCAGAGTGGTTTCTGAGGAAGCATTGGAAGGTCGTGGTTCAGTCAGGATTAAAGGCTGTTTTTACCCTAGGAAATGCCGACTGGAGAGATGTAATGATCCCCTGGAAAGCGAAGAAGCAAGTATATCACAGGTATTATCATCTCTTTGGACTCGAAGAGCTGAAGAAGCTTGCGGAGATGGCGGGGTTTTGGGTAGAGAAGTGCTATTTTCTTGATAAGGGAGGCAAAGAGGTTGAAGACTGGCATAAAGCGAATAATTCGTTTTTGGTGGCAAGGAAGGTGATTTGTAAGGGGTAG", "aa": "MKNAYRALRYEGLLVMTNRAFSEWFLRKHWKVVVQSGLKAVFTLGNADWRDVMIPWKAKKQVYHRYYHLFGLEELKKLAEMAGFWVEKCYFLDKGGKEVEDWHKANNSFLVARKVICKG", "aa_hexdigest": "dbe893e7bf831d76cda159a2a321091b", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 532772, "stop": 533431, "observations": { "mutation": [ 533048 ], "stop": [ 533048 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A660MVG4", "query_cov": 1.0, "subject_cov": 0.4779116465863454, "identity": 0.958, "score": 248.0, "evalue": 1.03e-81, "gene_start": 1, "gene_end": 119, "reference_start": 131, "reference_end": 249, "reference_sequence": "MKIIDELEQFYTAEAQKYAQTRRKHRPDGERLLEALENLEMKSPKLLELGCGSGRLISYLDQAWERKFAYTGVDLSSGLLKYAKEANPGRKFVCSEMTSFLEGCKQESFDAVVACASFQHIPSAKARLAVMKNAYRALRYEGLLVMTNRAFSEWFLRKHWKVVLQSGLKAVFTLGNADWRDVMIPWKAKKQVYHRYYHLFGLEELEKLAEMAGFWVEECHFLDKGGKKVEDWHKANNSFLVARKVICKG" }, "paralog": false, "identity": 0.9497716894977168, "score": 436.0, "evalue": 5.13e-158, "description": "Internal stop codon at 533048. Nonsense mutation around 533048.", "psc": { "uniref90_id": "UniRef90_A0A660MVG4", "product": "Class I SAM-dependent methyltransferase", "uniref50_id": "UniRef50_A0A660MVG4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A660MVG4", "UniRef:UniRef50_A0A660MVG4" ] }, "pscc": { "uniref50_id": "UniRef50_A0A660MVG4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MVG4" ], "product": "Class I SAM-dependent methyltransferase" } }, "id": "NKOCPLAKPK_1007", "locus": "GCA003260355_00971" }, { "type": "cds", "sequence": "contig_1", "start": 533649, "stop": 533774, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCGATACACTCAAATGGTAGTAGAAGATATCTCGGACAGGAAAGACTGTGTACAAATGATACCGAGTGTGATCCTAGTGGTAGCTCTTGGAAGAATCAACTATCAAGTCCTAACGAACATCTAG", "aa": "MRYTQMVVEDISDRKDCVQMIPSVILVVALGRINYQVLTNI", "aa_hexdigest": "ebdbd3f708e8c45fd72e6808624c72e2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4725.575599999999, "isoelectric_point": 5.917302894592285 }, "id": "NKOCPLAKPK_1008", "locus": "GCA003260355_00972" }, { "type": "cds", "sequence": "contig_1", "start": 533901, "stop": 534158, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGTATGTGTCCTGAAGGGATTTACTGTAAATGGGTTCCGGTTCCTTGCACAAAAAGAAAATATAATAAAATTACAGGTCAATTTATTAGCCAAGAAATTATCTATAGATATGCAGAATGTCCTTGTCACCCAGGACGAGGGATGGAAGAGAGACCCAGCCTTCCTAGCACTCCGAGTACTCCGAGTGTTCCGAGTGTTCCGAGTGTTCCAGGTGTTCCAGGTGGTCCCGGAAGTCCAAGCGGACCTTCTTGTATAA", "aa": "MVCVLKGFTVNGFRFLAQKENIIKLQVNLLAKKLSIDMQNVLVTQDEGWKRDPAFLALRVLRVFRVFRVFQVFQVVPEVQADLLV", "aa_hexdigest": "564845f51cbd8bcc6a71ee7b2fea7ef7", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9848.692799999999, "isoelectric_point": 10.008603858947755 }, "id": "NKOCPLAKPK_1009", "locus": "GCA003260355_00973" }, { "type": "cds", "sequence": "contig_1", "start": 534162, "stop": 534266, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAACAGTGGATCCCTCAGTCTGCAGACATCGAAGTCAAACGAATGGAGGAAGAGTTCGTGTCCATACTATGTCATGGTGTAATGCTACAAGAGAAGAAATAG", "aa": "MEQWIPQSADIEVKRMEEEFVSILCHGVMLQEKK", "aa_hexdigest": "a236bd5ceeb90ba479a2a9aa729eb351", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4062.7108, "isoelectric_point": 4.796722221374512 }, "id": "NKOCPLAKPK_1010", "locus": "GCA003260355_00974" }, { "type": "cds", "sequence": "contig_1", "start": 534384, "stop": 534605, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATTTCAATTGTAAGCCATGATATATTTATCATAATAAGTGAGGGAATAACAGAGGTTGTAGACCCTGTCAGTCAAATAAGGATGAAGGGACTCGATCTGCAGGAGGACAACAAGAGAGATGTGAACTATGTGACCCTAAACCAGCGAATTCTCATTACGTGGGATGAAATTTTACAAATAAAACATGTAAATGGGAATGTGATGAAGGATTCAATCTGA", "aa": "MISIVSHDIFIIISEGITEVVDPVSQIRMKGLDLQEDNKRDVNYVTLNQRILITWDEILQIKHVNGNVMKDSI", "aa_hexdigest": "d5d82ea9a6ed1b214e801d6f8640bb90", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8405.634799999998, "isoelectric_point": 4.849809837341308 }, "id": "NKOCPLAKPK_1011", "locus": "GCA003260355_00975" }, { "type": "cds", "sequence": "contig_1", "start": 534583, "stop": 534927, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCAACAATCAATAAACAAGGGTTGCAGGAATTTATCACTCATGCAAGCACAAGGGGTGGGACAGCAACGACGATCAACTCAGGATTTAAAGCATGGACGACAACGGGATATAGTGAATATTCATGTAATTTTCATTGTGATGAAAGATTCGCATATAAACATGATACGAATACTACAGGCTCTTGTACTTTTTGTCAGCCTGGAACTTATTCATCAGGAGGTGTTGCACAATGTGCATCATGTACGCCAATAGCTAACGCAACATTCATCACAAACGGAACGAAAAACCGAACAGACCCATCTTCGAGTAGGTTGGATTGTGATTTCAATTGTAAGCCATGA", "aa": "MPTINKQGLQEFITHASTRGGTATTINSGFKAWTTTGYSEYSCNFHCDERFAYKHDTNTTGSCTFCQPGTYSSGGVAQCASCTPIANATFITNGTKNRTDPSSSRLDCDFNCKP", "aa_hexdigest": "623f75a14e81e53e945713d13e64f763", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12303.38339999998, "isoelectric_point": 7.547217750549317 }, "id": "NKOCPLAKPK_1012", "locus": "GCA003260355_00976" }, { "type": "cds", "sequence": "contig_1", "start": 535798, "stop": 535992, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGAGGAAAGGATAATTCTCTGGCTTCTGCCACAAATTCTATTGTCCTCGGAGGAAGGGGAACTGTAGCGGCTAGTGCAAATAACTTAGCGATTATTGGTGCTTCTGAGACGAGAGTTCCAACAGAAGGGATAAGTGCACCTAAAAAAGAAAATATCCTTGTAGCTGGAGGTCATGGGAATGTTGTAAGATGA", "aa": "MGGKDNSLASATNSIVLGGRGTVAASANNLAIIGASETRVPTEGISAPKKENILVAGGHGNVVR", "aa_hexdigest": "e14a339883378e6a87a77213b82b9c59", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6270.997900000001, "isoelectric_point": 9.690839195251463 }, "id": "NKOCPLAKPK_1013", "locus": "GCA003260355_00977" }, { "type": "cds", "sequence": "contig_1", "start": 536649, "stop": 536762, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAGGAATTTGGATCAATAGATACCACAAAGAAACTAAAGGACTTTGTGCAAGATTTACAGTCTATTCTACAGCAGAGCTCTTTGAATTCCTCAAAAAACTCATTCCTTAA", "aa": "MQGIWINRYHKETKGLCARFTVYSTAELFEFLKKLIP", "aa_hexdigest": "b383eb71af49d9f85b67137f5a216a80", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4433.202399999998, "isoelectric_point": 9.428968620300292 }, "id": "NKOCPLAKPK_1014", "locus": "GCA003260355_00978" }, { "type": "cds", "sequence": "contig_1", "start": 536763, "stop": 536909, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00702.32", "PFAM:PF13242.12" ], "nt": "ATGGTCAGTTCAGAAGAGGCAGGAATGGATAAACCTCATTCTTCTCCTTTCCTGCTTGCAGCCCACAAACTTGAGGTGCTTACCAAAGAGTGTATTATGGTCGGAGACAATATTCAGAAAGATATTGATGGAGCGCAATGGCTTTGA", "aa": "MVSSEEAGMDKPHSSPFLLAAHKLEVLTKECIMVGDNIQKDIDGAQWL", "aa_hexdigest": "a247e69c0c404d243ce2fbfd72a8fd2d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00702.32", "name": "Hydrolase", "length": 44, "aa_cov": 0.9166666666666666, "hmm_cov": 0.9772727272727273, "evalue": 1.2498093647778922e-08, "score": 31.966398239135742, "start": 3, "stop": 46 }, { "id": "PF13242.12", "name": "Hydrolase_like", "length": 37, "aa_cov": 0.7708333333333334, "hmm_cov": 1.0, "evalue": 1.4321401700058376e-08, "score": 30.99436378479004, "start": 10, "stop": 46 } ], "seq_stats": { "molecular_weight": 5284.027100000001, "isoelectric_point": 4.734824562072753 }, "id": "NKOCPLAKPK_1015", "locus": "GCA003260355_00979" }, { "type": "cds", "sequence": "contig_1", "start": 536985, "stop": 537134, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACAGCCAAATGAAGAATAAAATCCTGCCAAAAGATATTCTTCATATTTTTGATCGTTATTGGAAAGTCTTTTATAGCACGATCAAGAGAGAAAGAGCAAGTACAGAGCTTCTAAAATATCTCAAAAGAAAAGGGATAAAAACCTGA", "aa": "MNSQMKNKILPKDILHIFDRYWKVFYSTIKRERASTELLKYLKRKGIKT", "aa_hexdigest": "71ec4e5b717f9fc46695f5ebb9a07a5e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6031.149399999999, "isoelectric_point": 10.288783454895018 }, "id": "NKOCPLAKPK_1016", "locus": "GCA003260355_00980" }, { "type": "cds", "sequence": "contig_1", "start": 537186, "stop": 537365, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAATAAAGGCAGTGCTGTTTGACCTTGATGATACGCTTTTTGACTATGAATCTTGTAATCAAGAAGCGACAAAAGAAATTTTTAAAGAAATCCATAAGTTGACCAAGACTCCTTTAGAGCTTATCCCAATTTTATTTGAAATTGCACAGAAAGAGGTAAAAATGCAACTTTTGTGA", "aa": "MAIKAVLFDLDDTLFDYESCNQEATKEIFKEIHKLTKTPLELIPILFEIAQKEVKMQLL", "aa_hexdigest": "acb4ed041e9716011c311b109ca6f3f5", "start_type": "ATG", "rbs_motif": "TTATT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6898.062699999997, "isoelectric_point": 4.750057411193848 }, "id": "NKOCPLAKPK_1017", "locus": "GCA003260355_00981" }, { "type": "cds", "sequence": "contig_1", "start": 537508, "stop": 537678, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCAAAAGCAAAAAAAGCTGATCTCAAAACTGACATTTTGAATAGTCCAATAGGAAACCAAAACTTCGCTCATTCTGAGGAAGAAATCGTTTTCCAAGGGATCAGAACCCACAACCTCAAAGATATTGACCTCATTTTGCCGAAAAATAAGCTCATTACCATCACTTGA", "aa": "MPKAKKADLKTDILNSPIGNQNFAHSEEEIVFQGIRTHNLKDIDLILPKNKLITIT", "aa_hexdigest": "398ee18c479cbdc64c915632cb4d6f60", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6326.281799999999, "isoelectric_point": 8.106464195251466 }, "id": "NKOCPLAKPK_1018", "locus": "GCA003260355_00982" }, { "type": "cds", "sequence": "contig_1", "start": 537679, "stop": 538119, "strand": "+", "frame": 1, "gene": null, "product": "UvrABC system protein A", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3D0USC2", "UniRef:UniRef90_A0A3D0USC2" ], "nt": "GTGAGTGGATCGGGGAAATCTTCTCTGGCTTTTGAAACCATTTATAAAGAGGGACAATACAGGTATATTGAATCGCTTTCAAGCTATCTGAGGCAGTTTTTCAATCTTGGAGATAGACCAGAAATTGACTACTGTAGCGGACTTTCTCCTGCAATTGCGATTGAGCAAAACAAAAGAAGCGGAAATTCAAGGTCTACCGTAGGAACGCTTACCGAGATTGATGACTACATCAGGCTGCTGTTTGCAAAGCTCGGTGATACCTACTGTTATCACTGCGGTGCGAAGATTGAGCCAAAGACCATTGATCAGATCATGGATACGATCAAAACTGACTATCAAGGGCAGAAAATCTATCTTTTGAAAGAAAGCGGAAAGTTCTCAGAAAAGTCAGAATTATTGAAATTTGTCAAAAATAATAAACAAAAAGTAGAAAAAGGCTGA", "aa": "MSGSGKSSLAFETIYKEGQYRYIESLSSYLRQFFNLGDRPEIDYCSGLSPAIAIEQNKRSGNSRSTVGTLTEIDDYIRLLFAKLGDTYCYHCGAKIEPKTIDQIMDTIKTDYQGQKIYLLKESGKFSEKSELLKFVKNNKQKVEKG", "aa_hexdigest": "eff0bc5c3856944559e81235aa0819b4", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 537580, "stop": 538050, "observations": { "mutation": [ 537676 ], "stop": [ 537676 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A3D0USC2", "query_cov": 0.8424657534246576, "subject_cov": 0.7454545454545455, "identity": 0.821, "score": 216.0, "evalue": 1.19e-69, "gene_start": 1, "gene_end": 123, "reference_start": 43, "reference_end": 165, "reference_sequence": "MAKKIQAPIHNDNMITFQGVRTHNLRNIDIELPKNQIITITGVSGSGKSSLAFDTIYKEGQYRYIESLSSYLRQFFNLGERPDIDHCTGLSPAIAIEQNKKTGNSRSSVGTITEIDDYLRLLFAKLGETYCYSCGQEIKPQTINQIMDIIKSDYEGQKIYLLQES" }, "paralog": false, "identity": 0.782051282051282, "score": 261.0, "evalue": 6.87e-90, "description": "Internal stop codon at 537676. Nonsense mutation around 537676.", "psc": { "uniref90_id": "UniRef90_A0A3D0USC2", "product": "UvrABC system protein A", "uniref50_id": "UniRef50_A0A3D0USC2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A3D0USC2", "UniRef:UniRef50_A0A3D0USC2" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3D0USC2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3D0USC2" ], "product": "UvrABC system protein A" } }, "id": "NKOCPLAKPK_1019", "locus": "GCA003260355_00983" }, { "type": "cds", "sequence": "contig_1", "start": 538453, "stop": 538821, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF17755.6" ], "nt": "ATGAGCGAAAGCAAGGGAAAGCCAGTAATTCAGCGGTTTACCGACAAAATGTATTGTCCAAACTGTAATATCACTTATCCTGAATTTACGACTCAGCATTTTTCTCCTAATAGGCAGGAAGGAGCTTGTCCGCATTGTTTGGGGCTGGGTGAGATTTTGCAGGTGGAATATGATAAAATCATTGATCCTCAAAGTCCGCTTTTGGAGGCGATTTTGCCATGGAGAGAAAGTAATCTTGGACAGTCTGTTTTAAAAAAGCTCTGTGAGAAATACAGCATGGATGGCGAGAAAAAACGAGCGGATCAACCAGAATGGTTTTTGAATGTGATTATTGAGGGAGATGATGAGCTTTTGAGAGTCAATAGCTGA", "aa": "MSESKGKPVIQRFTDKMYCPNCNITYPEFTTQHFSPNRQEGACPHCLGLGEILQVEYDKIIDPQSPLLEAILPWRESNLGQSVLKKLCEKYSMDGEKKRADQPEWFLNVIIEGDDELLRVNS", "aa_hexdigest": "6d25ee033a6abb5fe09f7bb568aa20eb", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF17755.6", "name": "UvrA_DNA-bind", "length": 63, "aa_cov": 0.48360655737704916, "hmm_cov": 1.0, "evalue": 1.466127757694623e-06, "score": 24.988204956054688, "start": 58, "stop": 116 } ], "seq_stats": { "molecular_weight": 14032.853799999988, "isoelectric_point": 5.027147483825684 }, "id": "NKOCPLAKPK_1020", "locus": "GCA003260355_00984" }, { "type": "cds", "sequence": "contig_1", "start": 538891, "stop": 539286, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGACCGTTGATTTTCAGGCGATGCTGAGTATGAAGAGCTGTCCGCATTGTTTCGGTTCTAGGCTTAAAAAGGAGAGTTTGCACTGCTTTTTGTGTTTGAATACAGATCGTCATTGCGAGGGAGGAACGACCGAAGCAGTCGAAAAAGCCACAAAAAAGCTGAAAAAAACCTATGAAACGGCTAAAAATCAAGACTCTGGATTGGTTGGCTTTGCTCGCAAAGAGGGAGTTGAAGCATTGATCCCATCACTCTTTATCAATCAAGAAGGTTTACCTTTTGCAAGACCAGCAGATGAACAGCTTTTGAAGGTAAATATCCGAGATTTGCAGAGAATGGAACTGAATCATCTGATCAGCTTTTTAGACCTTTTCAAACAAGTAAGTAATAAACCTTGA", "aa": "MTVDFQAMLSMKSCPHCFGSRLKKESLHCFLCLNTDRHCEGGTTEAVEKATKKLKKTYETAKNQDSGLVGFARKEGVEALIPSLFINQEGLPFARPADEQLLKVNIRDLQRMELNHLISFLDLFKQVSNKP", "aa_hexdigest": "2ddde965315ffcabed4506dfdb423dbf", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14815.043499999983, "isoelectric_point": 8.619890403747561 }, "id": "NKOCPLAKPK_1021", "locus": "GCA003260355_00985" }, { "type": "cds", "sequence": "contig_1", "start": 539362, "stop": 539493, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGAAAAGAGGGGTAGATACGATTTCTGGAGGGGAGATTCAGAGGTTAAGACTAGTAAAACAGCTCGGTAATAAACTAACCGGGATTATTTATGTGCTGGATGAGCCGACGATTGGGCTTGATACCTGA", "aa": "MLKRGVDTISGGEIQRLRLVKQLGNKLTGIIYVLDEPTIGLDT", "aa_hexdigest": "aa4f9656c9cafe125ba02297c0a63e3b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4725.509800000001, "isoelectric_point": 8.189177131652833 }, "id": "NKOCPLAKPK_1022", "locus": "GCA003260355_00986" }, { "type": "cds", "sequence": "contig_1", "start": 539536, "stop": 539817, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGGAATACCATCATCGTCGTGGAGCATAATGAGGAATTTATCAAGGCTTCGGATTGGGTGGTAGAAATCGGTCCTGGAGCTGGAGATTTCGGTGGAGAGCTGATTTTTAACGGAAGCTATGAGGATTTTCTGCAAGCAAAAACGCTGACTTCCGACTACATTACTGGGGAAAAGAAAGTGAAGGTTGATTTTGAGCATAGTCCAAGCCACGACCGAGTGCAGATCAAGCACGCAAGTAAATATAATCTCAAGAATATTGATGTTAAGCTGAATCTCTGA", "aa": "MGNTIIVVEHNEEFIKASDWVVEIGPGAGDFGGELIFNGSYEDFLQAKTLTSDYITGEKKVKVDFEHSPSHDRVQIKHASKYNLKNIDVKLNL", "aa_hexdigest": "d1b9041efbd8c4c73e09282a7b25f30c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10437.587399999991, "isoelectric_point": 5.223753547668456 }, "id": "NKOCPLAKPK_1023", "locus": "GCA003260355_00987" }, { "type": "cds", "sequence": "contig_1", "start": 539827, "stop": 540768, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13304.12", "PFAM:PF00005.33" ], "nt": "GTGATCACTGGACCTTCTGGAGCCGGGAAAACGACCTTGATGTACACGACATTGTATAGATTTCTCAATGATAAGCAGAAATTCGTTCAGAGTTATATCAGACTTCAACTTTTGAAAAAAGGTTTGAGCTGGGAGGAAATCTTGGCTGCTCCCGTGATGAGAGCTGAGGAATACGCCCATTTTGAGAATCTCGCATTGCAAGAATTTTACCAAGATATCGGAGTAGAAAAAATCACCGGACACGAACAGATCAAAAACACGATCTATGTAGACCAGTCTAGCATTGGGAAAACTCCGAGAAGCTGCCCTGCGACTTTTGTGAGTGTCTTTGACAAAATCAGGCTCCTCTATGCAGGAACGACCGAAGCGAAGTATTTAGGCTTTACGAACTCGCATTTCAGCTTTAATTCTGAAAAAGGTGCCTGCCCTGCTTGTAATGGGTATGGCTACAAAAAGATTGAGCTGCAGTTTCTGCCTGATACCTATGTTCCTTGTGAGCTATGTCAAGGAAAGAGATATAAACCTGAAATTCTCGCGATCACTTGGAGAGGGAAGACAATTAGTCAGATTTTGGATATGTATATCTATGAAGCCTTGGACCTCTTTCATGAAATGGATCATATCAAACAGGAACTAGAACTGATGGTAGAAATCGGACTTGGCTATCTCAAGATGGGACAACCTGCACATACCCTTTCTGGTGGAGAATCACAGAGATTGAAGCTCGTGAAGCACCTTTTGAAAGAATACAGAGGGAATACGGTGTATTTCCTTGATGAGCCGACGGTTGGACTGCATCCTGCGGATATTGAAAGGCTTTTGAAAGTGCTGAAAAGATTTTTGGAGAAAGGAGATACGATCCTGATGATTGAGCATGATGAAGATATTTTGCAATTCGCAGATCATGTGATTAGGTTAGATAATGGGAAACTCGTAAAGTAA", "aa": "MITGPSGAGKTTLMYTTLYRFLNDKQKFVQSYIRLQLLKKGLSWEEILAAPVMRAEEYAHFENLALQEFYQDIGVEKITGHEQIKNTIYVDQSSIGKTPRSCPATFVSVFDKIRLLYAGTTEAKYLGFTNSHFSFNSEKGACPACNGYGYKKIELQFLPDTYVPCELCQGKRYKPEILAITWRGKTISQILDMYIYEALDLFHEMDHIKQELELMVEIGLGYLKMGQPAHTLSGGESQRLKLVKHLLKEYRGNTVYFLDEPTVGLHPADIERLLKVLKRFLEKGDTILMIEHDEDILQFADHVIRLDNGKLVK", "aa_hexdigest": "9740060dbf27ccbaf79f059aa1e3d3e9", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13304.12", "name": "AAA_21", "length": 56, "aa_cov": 0.17891373801916932, "hmm_cov": 1.0, "evalue": 2.9116899093451725e-09, "score": 33.8269157409668, "start": 227, "stop": 282 }, { "id": "PF00005.33", "name": "ABC_tran", "length": 53, "aa_cov": 0.16932907348242812, "hmm_cov": 0.9433962264150944, "evalue": 3.802362919339103e-10, "score": 36.9654541015625, "start": 210, "stop": 262 } ], "seq_stats": { "molecular_weight": 35890.10710000001, "isoelectric_point": 6.564585304260253 }, "id": "NKOCPLAKPK_1024", "locus": "GCA003260355_00988" }, { "type": "cds", "sequence": "contig_1", "start": 540791, "stop": 540943, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAAAACTGACCTATACTACAGTTGACACTCCCTTTCAGTTTGAAATTCCAAAAATTAAGGGATCAAGGTTTATTACCTCAGTTTTCCCTATTGATAGTAAGGATCATGTTCCTGAGTATCTTTCTCAGATCCAAAAACAGTATTTCTGA", "aa": "MEKLTYTTVDTPFQFEIPKIKGSRFITSVFPIDSKDHVPEYLSQIQKQYF", "aa_hexdigest": "8da20ecf028173c4ce051b7f9408666f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5942.744400000001, "isoelectric_point": 6.529743003845215 }, "id": "NKOCPLAKPK_1025", "locus": "GCA003260355_00989" }, { "type": "cds", "sequence": "contig_1", "start": 541247, "stop": 541405, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATCCACTATCGTTATGAGGAAGTTTCCCGAGTTCAGCATCTTTTTGCTAAATATCAAGCGATCCCCTATTATGAGGAATATGGAGATAGCATTACACAAGGGGTTGAGGTAAATTATAGCTTTTTTCAGAACCTGACTAAGGATTTGACCTCATGA", "aa": "MIHYRYEEVSRVQHLFAKYQAIPYYEEYGDSITQGVEVNYSFFQNLTKDLTS", "aa_hexdigest": "a817c22c7a1189868004aaf9b99ce89f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6272.8706, "isoelectric_point": 5.048121070861816 }, "id": "NKOCPLAKPK_1026", "locus": "GCA003260355_00990" }, { "type": "cds", "sequence": "contig_1", "start": 541515, "stop": 543404, "strand": "-", "frame": 2, "gene": "thrS", "product": "threonine--tRNA ligase", "db_xrefs": [ "EC:6.1.1.3", "GO:0004829", "GO:0005524", "GO:0005737", "GO:0006435", "KEGG:K01868", "SO:0001217", "UniParc:UPI000DBF4DE1", "UniRef:UniRef100_A0A328EGF7", "UniRef:UniRef50_A0A1F5EME7", "UniRef:UniRef90_A0A328EGF7" ], "nt": "ATGGAACAAGCATTACTCAAAAAAAGGCATTCGCTCTCGCATATCCTTGCACAGGCGGTGCAGAGAGAGCAGCAACGAGATGTGGAAGTTGCGATCGGACCTGCGATTGATAACGGATTTTATTATGACTTTCTCTTTAGCTCTGAAAAGCAGATCAAGGAGGAGGATTTGAAAAAGATCCAAAATCAGATGGAAAAAATCGTGAAAGAAAATCAGGATTTCGTTCTTTTTTCGCTTTCTGATGCGGAATCAAAAAAACTCGTTACTGAGTTGATGAAGCAGCAATACAAAGAAGAGATGAGAAGCGAATTTGCGGCAGCAGGAGAGGAAATTACCTTCTATGCCAATACAATCGTGGAGGGTGCGAAAGATGCCTTACTCAAGGGAATAGACCCATCTTATCGTGAGTATTATGAAAAGGTAACTGCGTATTTGCAGGAAAAGTTCCCTGAAAAGTTTGCTGGGAAGTTCATTACATTCCTTGATATGTGTGAAGGTCCGCATGTGGAAAACACCAAAGAGCTTGATAGCAAGGCTTTCAAACTTGATAAACTCGCAGGGGCGTACCGAAGAGGAAATTCTGATAATGTGATGATGGTTAGAGTCTATGGTTTAGCATTTGATACCAAAGAAGAGCTTCAAAAATATCAAACCCTTCTTGAAGAAGCCAAAAAAAGAGATCACAGAATCTTGGGTCAAAAGCTGAAAATTTTTACCATTTCACCGCTGGTTGGATCAGGATTACCACTTTTGCAGCCGCATGGAATGATTATTAGAAAAGAAGTTGAGGATTACCTTTGGGAGCTTCACAAAGATAAAGGCTATCAAAGAGTATGGACTCCTCACCTTGCCAAAGAAGACCTTTACAAAACTTCAGGGCATGCGGATAAGTTTGGAGATGAGCTTTTTAGAGTAAAGGGAAAGGAGGAAAACTTCTTTATGAAGCCGATGAACTGTCCTCATCATATGCAGATTTTCGCTGATAATCAGTTTAGCTATCGTGATATGCCGGTTCGTTATTTCGAGCCTGCAACCGTCTATAGAGATGAAAAGACTGGTCAGCTTTCTGGTTTGACTCGTGTGAGATCTATTACTCAAGATGATGGGCATCTTTTCTGCCGTGTGAGCCAAATTACTGAGGAAGTAGGAACTATCGTGGAAATCATCAAAAAATTCTATTCAACCCTCGGAATGATCAATGATTACCGAGTAAGGCTTTCTATTCGTGGTCCAGAAGGGAAATATCTCGGAGATGATGCGGTTTGGGAGCAGGCAGAGGGGGCTTTGAGAGAGGCATCTGAGAAATATCAGCTTCCTTACAAGATCGGAGTAGGGGAGGCGGCTTTCTACGGTCCAAAACTGGATTTTATGTTCAAAGATGCGATCGGTAGAGAATGGCAACTTGCGACGATTCAGTGTGATTTCAATCTCCCAGTCAGATTTGATCTTTCTTTTACCAATGAACAAGGAGAAAAGGAGAGACCTGTGGTGATTCATAGAGCGATTTCTGGGTCTTTGGAGAGGTTTATGGGCGTGATGATTGAGCATTTCGGAGGAGCGTTTCCGCTATGGATTGCACCGGTTCAGTTCCAGATCGTGCCAGTCGCAGAAAAGTTTAATGATTACGCTTTCCAAGTGGCTTCACAGCTCAAAAAAGCTGGCTTTAGAGTGAAGGTAGATGAGGGAAACGATAGTTTCTCCAAGAAAATCCGTAATGCTGAAATGATGAAAGTTCCTTATATTCTCATCGTTGGAGAGAAAGAAGAATCCGCAAATGAGGTTTCTGTCAGAGAATTTAGGAGCAAGAAGCAATATGTGCTGGGAGTAGAGGAGCTTATGAAGCAGGTTGAGGAGGAAAGAAGTAAAAGAAAACTCGCATCAGAGCATTAG", "aa": "MEQALLKKRHSLSHILAQAVQREQQRDVEVAIGPAIDNGFYYDFLFSSEKQIKEEDLKKIQNQMEKIVKENQDFVLFSLSDAESKKLVTELMKQQYKEEMRSEFAAAGEEITFYANTIVEGAKDALLKGIDPSYREYYEKVTAYLQEKFPEKFAGKFITFLDMCEGPHVENTKELDSKAFKLDKLAGAYRRGNSDNVMMVRVYGLAFDTKEELQKYQTLLEEAKKRDHRILGQKLKIFTISPLVGSGLPLLQPHGMIIRKEVEDYLWELHKDKGYQRVWTPHLAKEDLYKTSGHADKFGDELFRVKGKEENFFMKPMNCPHHMQIFADNQFSYRDMPVRYFEPATVYRDEKTGQLSGLTRVRSITQDDGHLFCRVSQITEEVGTIVEIIKKFYSTLGMINDYRVRLSIRGPEGKYLGDDAVWEQAEGALREASEKYQLPYKIGVGEAAFYGPKLDFMFKDAIGREWQLATIQCDFNLPVRFDLSFTNEQGEKERPVVIHRAISGSLERFMGVMIEHFGGAFPLWIAPVQFQIVPVAEKFNDYAFQVASQLKKAGFRVKVDEGNDSFSKKIRNAEMMKVPYILIVGEKEESANEVSVREFRSKKQYVLGVEELMKQVEEERSKRKLASEH", "aa_hexdigest": "90529fc2361d82b70ce82c951e0840d8", "start_type": "ATG", "rbs_motif": "TTCTT", "ups": { "uniparc_id": "UPI000DBF4DE1", "uniref100_id": "UniRef100_A0A328EGF7", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBF4DE1", "UniRef:UniRef100_A0A328EGF7" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EGF7", "uniref90_id": "UniRef90_A0A328EGF7" }, "psc": { "uniref90_id": "UniRef90_A0A328EGF7", "gene": "thrS", "product": "threonine--tRNA ligase", "ec_ids": [ "6.1.1.3" ], "uniref50_id": "UniRef50_A0A1F5EME7", "kegg_orthology_id": "K01868", "go_ids": [ "GO:0004829", "GO:0005524", "GO:0005737", "GO:0006435" ] }, "pscc": { "uniref50_id": "UniRef50_A0A1F5EME7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1F5EME7" ], "product": "Threonine--tRNA ligase" }, "genes": [ "thrS" ], "id": "NKOCPLAKPK_1027", "locus": "GCA003260355_00991" }, { "type": "cds", "sequence": "contig_1", "start": 543709, "stop": 543906, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAATTCTAGAAGTTCCGAAAGATCATTATTTGGTCAAAAGTTTTGCAGTATATGGAAAGGTCGCACATATCCGGTGGAAGAGTAGCGAAGAGTTAGCAACGCTTATTAGTGCCTTATTGGAAGTAAATCCTACCCATGAAGTAGTTGCAATGATCACTCCTGAAGAGGGAGGAACAATCCTCATCTTCCGTTAA", "aa": "MEILEVPKDHYLVKSFAVYGKVAHIRWKSSEELATLISALLEVNPTHEVVAMITPEEGGTILIFR", "aa_hexdigest": "fefd69325fa2753cdb8abb5c8e6ea130", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7321.471299999996, "isoelectric_point": 5.473049354553221 }, "id": "NKOCPLAKPK_1028", "locus": "GCA003260355_00992" }, { "type": "cds", "sequence": "contig_1", "start": 544353, "stop": 544646, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCATGGAGGCTATGAAAAAAAGAAAGCACTACTCCTCAATTTTCTGACGGCATTAAGTGCAGTTATTGGAGTAGTGATTACGCTCAGTATTGGGCATTTTGCACATGATATTGAGATGATCCTGCTTCCAATTTCTCTTGGAATGTTCGTATATATAGCGGGAACCGATTTAATACCAGAGGTTAATAAGCATAACGAAAATCTGAAATTTTCTCTCGTTCAGGTTGTGATTTTCGTTGTTGCTATCTTAATTACCTATGCATTAGCACATTTTGCAGAACATACTCATTAG", "aa": "MHGGYEKKKALLLNFLTALSAVIGVVITLSIGHFAHDIEMILLPISLGMFVYIAGTDLIPEVNKHNENLKFSLVQVVIFVVAILITYALAHFAEHTH", "aa_hexdigest": "c27504814e113099716a47fd5f1e03b2", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10695.604699999989, "isoelectric_point": 6.345414161682128 }, "id": "NKOCPLAKPK_1029", "locus": "GCA003260355_00993" }, { "type": "cds", "sequence": "contig_1", "start": 544662, "stop": 545003, "strand": "-", "frame": 2, "gene": null, "product": "ZIP family metal transporter", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EIN3", "UniRef:UniRef90_A0A328EIN3" ], "nt": "GTGGCATTTGCCGCGGCAACAATGCTCGGAAATGCCTTTTTTCACTTTCTGCCAGAAATTATTGAGCATCGAGAAGGAGAAAACCCACAAATTATCGGACTTTGGATTATCTTTGGAGTGCTTATCGGCTTATTGACTGAGAAAGTTCTTCATTGGAATCATTGTCATCATAGCCCAGACAAAGCACATCCACATACCTTCGCTAAAATGAGCCTTTTAGGAGATTTTATCCATAATTTAATGGATGGTATGGTTGTTGGTGCAGGCTTCCTCGTTTCTATTCCGACGGGAATTGCTACATCTCTAGCCATCCTAATGCATGAAGTTCCTCAAGAACTCTGA", "aa": "MAFAAATMLGNAFFHFLPEIIEHREGENPQIIGLWIIFGVLIGLLTEKVLHWNHCHHSPDKAHPHTFAKMSLLGDFIHNLMDGMVVGAGFLVSIPTGIATSLAILMHEVPQEL", "aa_hexdigest": "afefe87c95c60b77d6b8cad7c0ac8d74", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 544359, "stop": 545120, "observations": { "mutation": [ 544664, 545027 ], "stop": [ 544664, 545027 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EIN3", "query_cov": 1.0, "subject_cov": 0.44313725490196076, "identity": 0.973, "score": 233.0, "evalue": 5.83e-76, "gene_start": 1, "gene_end": 113, "reference_start": 40, "reference_end": 152, "reference_sequence": "MEVWIYSIVSAVIISFCSLLGIVFLGSKKGLGEKVIVALVAFAAATMLGNAFFHFLPEIIEHREGENPQIIGLWIIFGVLIGLLTEKVLHWNHCHHSPDKAHPHTFAKMSLLGDFIHNLMDGMVIGASFLVSIPTGIATSLAILMHEVPQELGDYAILVHGGYEKKKALLLNFLTALSAVIGVVITLSIGHFAHDIEMILLPISLGMFVYIAGTDLIPEVNKHNENLKFSLVQVVIFVIAILITYALAHFAEHAH" }, "paralog": false, "identity": 0.9762845849802372, "score": 491.0, "evalue": 8.4e-180, "description": "Internal stop codon at 544664, 545027. Nonsense mutation around 544664, 545027.", "psc": { "uniref90_id": "UniRef90_A0A328EIN3", "product": "ZIP family metal transporter", "uniref50_id": "UniRef50_A0A328EIN3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EIN3", "UniRef:UniRef50_A0A328EIN3" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EIN3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EIN3" ], "product": "ZIP family metal transporter" } }, "id": "NKOCPLAKPK_1030", "locus": "GCA003260355_00994" }, { "type": "cds", "sequence": "contig_1", "start": 545507, "stop": 545692, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTGTATGAGGGGAAAACACTTGATAGAGGAGCGAGAACAAGAGTAATGGACGCCTTCGTGGTTTTGGATAGAGAACAATACATCAAATTAACCGGCATCACCCCAGATCCCAAAGCGATCCCTAAAGTTCAACCCTATGAACCTAGTGCAGAAGATCAAGCTCAGTTCAGAAAGGTCGCATGA", "aa": "MVYEGKTLDRGARTRVMDAFVVLDREQYIKLTGITPDPKAIPKVQPYEPSAEDQAQFRKVA", "aa_hexdigest": "c5431a1a6745ca88b4f6ed8024b8c24c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6938.896100000004, "isoelectric_point": 8.034259605407716 }, "id": "NKOCPLAKPK_1031", "locus": "GCA003260355_00995" }, { "type": "cds", "sequence": "contig_1", "start": 545810, "stop": 545917, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCTCTTGCTTGAAGGAGATGATGTTGTGCAAGGATTCGTCAATTATATTGACAAAAAAATGGCAGGAAATGCAAATAGGAATTTTATCTGCTCTACGGAAGATTGA", "aa": "MLLLEGDDVVQGFVNYIDKKMAGNANRNFICSTED", "aa_hexdigest": "b94d7d5a4c20410d6c7efa64fac68b46", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3921.3904, "isoelectric_point": 4.366337394714355 }, "id": "NKOCPLAKPK_1032", "locus": "GCA003260355_00996" }, { "type": "cds", "sequence": "contig_1", "start": 545996, "stop": 546358, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGCTTTGCTTACAAGGAACTCTTGATAGAAAAAAGAGGAGGCAGACCGATTAGGGTGATAAAAGTCGTATTGGACGGGGAGCATTTTGTCGTAGCATCTCCCGCTAAGGACGGAGGAGCAACAACCGAGCAGCTCGCAAAAAACGTTGGCGGAGATACGGCAATTAACGGTGCATTCTTCTGTCCGGATGATTATTCCTCTTGTAAAATCGACGGGAAAAGACTGACGCATACTATTTCTGAGAGAGTGTTCCTTGGAAATGGTAAAGATTGGAGTAGATTCCGAGGAGATACGAGTATTAGAATGATATTTTCCTTTGATAAACAGGGAAATCCATTTTTTGTACAGAGAAATTCATGA", "aa": "MSFAYKELLIEKRGGRPIRVIKVVLDGEHFVVASPAKDGGATTEQLAKNVGGDTAINGAFFCPDDYSSCKIDGKRLTHTISERVFLGNGKDWSRFRGDTSIRMIFSFDKQGNPFFVQRNS", "aa_hexdigest": "ac008fbd4a7b1ad602640af1a7cb388f", "start_type": "ATG", "rbs_motif": "TTTTT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13335.991699999986, "isoelectric_point": 9.26135082244873 }, "id": "NKOCPLAKPK_1033", "locus": "GCA003260355_00997" }, { "type": "cds", "sequence": "contig_1", "start": 546473, "stop": 546661, "strand": "-", "frame": 3, "gene": "recX", "product": "Regulatory protein RecX", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A288DCT8", "UniRef:UniRef90_A0A328EK04" ], "nt": "TTGTCTGATAAGTATGCAGAGGATATTCAGGAAGGAATTTTGCAAGGTATTCAGAGAGAGATTAGAAATTATAAAAAAAGAGGAACAGACGGCTTTGAGATTATTCAAAAACTAATGAGAAAAGGCTATAAACTCAGCGATATCAAAAAAGTAATCAAAGAAAATACCAAAGAGAACGAGGAATACTAA", "aa": "MSDKYAEDIQEGILQGIQREIRNYKKRGTDGFEIIQKLMRKGYKLSDIKKVIKENTKENEEY", "aa_hexdigest": "b3a5e756a53742305429ae1605f9e6ac", "start_type": "TTG", "rbs_motif": null, "genes": [ "recX" ], "truncated": "3-prime", "pseudogene": { "start": 546473, "stop": 546931, "observations": { "mutation": [ 546730 ], "stop": [ 546730 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EK04", "query_cov": 1.0, "subject_cov": 0.40789473684210525, "identity": 0.9840000000000001, "score": 120.0, "evalue": 1.96e-33, "gene_start": 1, "gene_end": 62, "reference_start": 91, "reference_end": 152, "reference_sequence": "MGKSCYEYAIGIITKYPKTEKELRIKLYQQGYDSEMVLRTLEKLKSENFVNDLLFAESYLNSEVMKKGKPLFLIKKKLELKGIDKQLLNKLSDKYAEDIQEGILQGIQREIRNYKKRGTDGFEIIQKLMRKGYKLSDIKKVIKENTKENEEY" }, "paralog": false, "identity": 0.9802631578947368, "score": 295.0, "evalue": 9.65e-105, "description": "Internal stop codon at 546730. Nonsense mutation around 546730.", "psc": { "uniref90_id": "UniRef90_A0A328EK04", "product": "Regulatory protein RecX", "uniref50_id": "UniRef50_A0A288DCT8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EK04", "UniRef:UniRef50_A0A288DCT8" ] }, "pscc": { "uniref50_id": "UniRef50_A0A288DCT8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A288DCT8" ], "product": "Regulatory protein RecX" } }, "id": "NKOCPLAKPK_1034", "locus": "GCA003260355_00998" }, { "type": "cds", "sequence": "contig_1", "start": 546728, "stop": 546931, "strand": "-", "frame": 3, "gene": "recX", "product": "Regulatory protein RecX", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1V5ZQB9" ], "nt": "ATGGGAAAAAGCTGTTATGAATATGCAATTGGGATTATTACTAAATATCCAAAGACAGAGAAGGAACTGAGAATAAAGCTTTATCAGCAGGGATATGATTCAGAAATAGTTTTGAGAACCTTGGAAAAATTAAAGTCTGAAAATTTTGTAAACGATCTCCTTTTTGCAGAAAGTTATCTCAATAGCGAGGTAATGAAAAAGTGA", "aa": "MGKSCYEYAIGIITKYPKTEKELRIKLYQQGYDSEIVLRTLEKLKSENFVNDLLFAESYLNSEVMKK", "aa_hexdigest": "3c12254c1a91187b616061f0f44d0646", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A1V5ZQB9", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.7909999999999999, "score": 112.0, "evalue": 2.31e-31, "valid": false, "product": "Regulatory protein RecX", "uniref50_id": "UniRef50_A0A1V5ZQB9" }, "pscc": { "uniref50_id": "UniRef50_A0A1V5ZQB9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1V5ZQB9" ], "product": "Regulatory protein RecX" }, "genes": [ "recX" ], "id": "NKOCPLAKPK_1035", "locus": "GCA003260355_00999" }, { "type": "cds", "sequence": "contig_1", "start": 547008, "stop": 547121, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGATTGAAAAAACGAAATGCTGTAAGGGACACGATGGGAAGAGAAAAAATGGCTCAGGAGGTCAAGGCAGACCAAGAGCAAAAACTAGACGACTTGCTTGGAAAATTGTAA", "aa": "MRLKKRNAVRDTMGREKMAQEVKADQEQKLDDLLGKL", "aa_hexdigest": "6196540ffd85aee445012de88271701e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4344.0518999999995, "isoelectric_point": 9.693224525451662 }, "id": "NKOCPLAKPK_1036", "locus": "GCA003260355_01000" }, { "type": "cds", "sequence": "contig_1", "start": 547476, "stop": 547586, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATACGATGCTTTTTAACTTTCAGGCTTTTGTTGATGAAATGAGAGAAAAGCCAGACAAAAAGGAAATTGTAGAAAAATACGAAAAGCGATATGGTCCGATCCAATGA", "aa": "MDTMLFNFQAFVDEMREKPDKKEIVEKYEKRYGPIQ", "aa_hexdigest": "1926c653199edc130f80fdb0e36f50c2", "start_type": "ATG", "rbs_motif": "TTTTTA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4455.095500000002, "isoelectric_point": 5.179930686950683 }, "id": "NKOCPLAKPK_1037", "locus": "GCA003260355_01001" }, { "type": "cds", "sequence": "contig_1", "start": 547833, "stop": 548099, "strand": "-", "frame": 2, "gene": "ftsL", "product": "Cell division protein FtsL", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MSL0", "UniRef:UniRef90_A0A660MSL0" ], "nt": "ATGATACCCTGGATAAAAGATCAAGTTAAACGCTATGGAACACTCATAAAACGAGGACTATTGGTTATAAATATCGTTCTTATGTTGATGGCTGCACAAATTTTGGTAAACTATACGACTATCATCGAAAATACCGTCACGATCAAAGCTCAGACCGCTAGTGTCGAAGAGGAGATGGACTATATCAAAAATTATCAGCTCAAATACCTTAATTCTGATCATGCAAAATTTTTCCTTTCCCACGAAAATAATATCCTTAGCGATTGA", "aa": "MIPWIKDQVKRYGTLIKRGLLVINIVLMLMAAQILVNYTTIIENTVTIKAQTASVEEEMDYIKNYQLKYLNSDHAKFFLSHENNILSD", "aa_hexdigest": "46896128467f06d0adede9d16895c71e", "start_type": "ATG", "rbs_motif": null, "genes": [ "ftsL" ], "truncated": "5-prime", "pseudogene": { "start": 547710, "stop": 548099, "observations": { "mutation": [ 547772, 547835 ], "stop": [ 547772, 547835 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A660MSL0", "query_cov": 1.0, "subject_cov": 0.6821705426356589, "identity": 1.0, "score": 166.0, "evalue": 1.11e-51, "gene_start": 1, "gene_end": 88, "reference_start": 1, "reference_end": 88, "reference_sequence": "MIPWIKDQVKRYGTLIKRGLLVINIVLMLMAAQILVNYTTIIENTVTIKAQTASVEEEMDYIKNYQLKYLNSDHAKFFLSHENNILSDGESIIAFKKQTEKSDSISEKPGEEKKTPREERKAYIQEKLE" }, "paralog": false, "identity": 0.9767441860465116, "score": 243.0, "evalue": 1.87e-84, "description": "Internal stop codon at 547772, 547835. Nonsense mutation around 547772, 547835.", "psc": { "uniref90_id": "UniRef90_A0A660MSL0", "product": "Cell division protein FtsL", "uniref50_id": "UniRef50_A0A660MSL0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A660MSL0", "UniRef:UniRef50_A0A660MSL0" ] }, "pscc": { "uniref50_id": "UniRef50_A0A660MSL0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MSL0" ], "product": "Cell division protein FtsL" } }, "id": "NKOCPLAKPK_1038", "locus": "GCA003260355_01002" }, { "type": "cds", "sequence": "contig_1", "start": 548312, "stop": 548581, "strand": "+", "frame": 2, "gene": "mutL", "product": "DNA mismatch repair protein MutL", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A920GZR9" ], "nt": "GTGGAAAATTCCCTTGATGCAGGAGCTACTGAGCTGATTATTACGATCAATGATGGTGGAAAATCCTTTCTTTCTCTCCAAGATAATGGTTGTGGAATTGAGCTTTCTGATATGGATCTCCTGCTTGAAAGGTATGCAACCTCTAAAATTGATTCAGAGCAAGACCTTTTTTCTTTGAAGAGTTATGGATTTCGTGGAGAAGCATTGGCAAGTATTGCGGAAGTCAGCAAAATCTCAGTTTTGACCAAGACAAAATATGCAGAAATCTGA", "aa": "MENSLDAGATELIITINDGGKSFLSLQDNGCGIELSDMDLLLERYATSKIDSEQDLFSLKSYGFRGEALASIAEVSKISVLTKTKYAEI", "aa_hexdigest": "fee70f007df4a806f53471e8120ab1c8", "start_type": "GTG", "rbs_motif": "TTCTT", "psc": { "uniref90_id": "UniRef90_A0A920GZR9", "query_cov": 0.9438202247191011, "subject_cov": 0.84, "identity": 0.512, "score": 80.5, "evalue": 6.06e-18, "valid": false, "product": "DNA mismatch repair protein MutL", "uniref50_id": "UniRef50_A0A920GZR9" }, "pscc": { "uniref50_id": "UniRef50_A0A920GZR9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A920GZR9" ], "product": "DNA mismatch repair protein MutL" }, "genes": [ "mutL" ], "id": "NKOCPLAKPK_1039", "locus": "GCA003260355_01003" }, { "type": "cds", "sequence": "contig_1", "start": 548591, "stop": 548875, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A9E5GSN0" ], "nt": "GTGCGTCGTGGAAATGAAAACGTGATCAAACATCTCGGAGTTCCTTTTGAACATGGGACTTTGATTACGGTTGAAGATTTGTTTTATAATGTCCCAGCAAGGCTGAAATTTCTCAAGTCAGCGCAAACCGAATTTTATTATTGCTACAATTATTTTGTAGATATTGCCCTTTATCACTATGATAAGGCCTTTATCTTGAAGAAGAATGATAAACTGGTCTTCGATCTCAAGCCAGCTCGTGATTTACAAAGCAGAATTTTGGATCTTTTCAAAAAAGACTGGTGA", "aa": "MRRGNENVIKHLGVPFEHGTLITVEDLFYNVPARLKFLKSAQTEFYYCYNYFVDIALYHYDKAFILKKNDKLVFDLKPARDLQSRILDLFKKDW", "aa_hexdigest": "6c5ad10a254f5f562513f9d1d8f6115e", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A948C517", "query_cov": 0.9574468085106383, "subject_cov": 0.8256880733944955, "identity": 0.622, "score": 121.0, "evalue": 5.11e-34, "valid": false, "uniref50_id": "UniRef50_A0A9E5GSN0" }, "pscc": { "uniref50_id": "UniRef50_A0A9E5GSN0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A9E5GSN0" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11309.983399999985, "isoelectric_point": 8.990196800231931 }, "id": "NKOCPLAKPK_1040", "locus": "GCA003260355_01004" }, { "type": "cds", "sequence": "contig_1", "start": 548981, "stop": 549217, "strand": "+", "frame": 2, "gene": null, "product": "DNA mismatch repair protein S5 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A351Y4P8" ], "nt": "GTGAATGAGCGTCCCATCCAAGATAAAATCATTAGGAAAGCTCTCATGGATGCTTATGCAAGACAGATCACACCTGGGGAATATCCGATGGCAATTTTGATGTTCAAGATAGAACCAAGTGAGGTGGACGTGAATGTGCATCCTGCGAAATTACAGGTTAAATTTGCTGATTCTCAGATGATTTATCAAGTCGTCTATCAGACAATTTCTGAACTTTTGGGGAAACATAAGATTTGA", "aa": "MNERPIQDKIIRKALMDAYARQITPGEYPMAILMFKIEPSEVDVNVHPAKLQVKFADSQMIYQVVYQTISELLGKHKI", "aa_hexdigest": "60e1b38de69b7d6708a168e73d575623", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A351Y4P8", "query_cov": 0.9358974358974359, "subject_cov": 0.948051948051948, "identity": 0.6579999999999999, "score": 103.0, "evalue": 1.09e-27, "valid": false, "product": "DNA mismatch repair protein S5 domain-containing protein", "uniref50_id": "UniRef50_A0A351Y4P8" }, "pscc": { "uniref50_id": "UniRef50_A0A351Y4P8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A351Y4P8" ], "product": "DNA mismatch repair protein S5 domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_1041", "locus": "GCA003260355_01005" }, { "type": "cds", "sequence": "contig_1", "start": 549138, "stop": 549368, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAGAGAAAAAATCTGACTTTTCACTCCCTTTTTCTTCCGCTGTCAAAAAAGCTGAAAAATCTGGCTTCTGAGACGGAAACGCGGAAGATTGAGGAAAAGATGGACGAGAAGCTGTGCCTTTTCATTGAAAAAACTCTGATCCGACGAATCAAATCTTATGTTTCCCCAAAAGTTCAGAAATTGTCTGATAGACGACTTGATAAATCATCTGAGAATCAGCAAATTTAA", "aa": "MERKNLTFHSLFLPLSKKLKNLASETETRKIEEKMDEKLCLFIEKTLIRRIKSYVSPKVQKLSDRRLDKSSENQQI", "aa_hexdigest": "9879f382007df95bd4b70f2529c870cb", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9080.561399999991, "isoelectric_point": 9.809589958190916 }, "id": 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10441.006899999993, "isoelectric_point": 4.544527626037598 }, "id": "NKOCPLAKPK_1043", "locus": "GCA003260355_01007" }, { "type": "cds", "sequence": "contig_1", "start": 549818, "stop": 550042, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTATCTTGAAGAAATCACTTTTGATCATCTTTTGGATGGGGTCTATGCAACGAAAGCCTGCAAAGCATCGATTAAAGCTGGACATAAACTTTCCTATCTCCAGATGCAACAACTCGTTCAAGATGGATTTGAATTTATCCCTGGGATGTTCGTTTGTCAGCACGGAAGACCGTTTTTTGTGAAGATGGATAAGAAGCATATTGATGGGCTTTTTGATAGATAA", "aa": "MYLEEITFDHLLDGVYATKACKASIKAGHKLSYLQMQQLVQDGFEFIPGMFVCQHGRPFFVKMDKKHIDGLFDR", "aa_hexdigest": "cc609dc26679fb7c2b87e4c5681b0a57", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8583.938199999997, "isoelectric_point": 6.8886812210083015 }, "id": "NKOCPLAKPK_1044", "locus": "GCA003260355_01008" }, { "type": "cds", "sequence": "contig_1", "start": 550146, "stop": 550253, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02873.21" ], "nt": "ATGAAACTCGCAGCTGGACAGCTCATAGAACTCTGTGGACTCAAAGGATACCAAGAGGGAAACATAAAAATCTCTGATCAACACGCATTAATTTTGATCAATCTTTGA", "aa": "MKLAAGQLIELCGLKGYQEGNIKISDQHALILINL", "aa_hexdigest": "52d4d38e47b973a43a5c9fb54983540f", "start_type": "ATG", "rbs_motif": "TTCTA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02873.21", "name": "MurB_C", "length": 31, "aa_cov": 0.8857142857142857, "hmm_cov": 1.0, "evalue": 2.800384185907303e-10, "score": 36.7681999206543, "start": 4, "stop": 34 } ], "seq_stats": { "molecular_weight": 3809.4992, "isoelectric_point": 6.510758781433105 }, "id": "NKOCPLAKPK_1045", "locus": "GCA003260355_01009" }, { "type": "cds", "sequence": "contig_1", "start": 550548, "stop": 550676, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCGTGATAATAAAGTTTTCTTTGAGTTCCTCTTTGAAGATGCTTTTGCGATAGCCAAATTTACATTCCTCATTCGTGAGTTTCTGAATGCTCTTAGTCTGCGTATTAACGCCGATGACCTCATATAA", "aa": "MRDNKVFFEFLFEDAFAIAKFTFLIREFLNALSLRINADDLI", "aa_hexdigest": "d801f12792a5cb5cbfdcbb5dbf0e940e", "start_type": "ATG", "rbs_motif": "TTTA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5015.7805, "isoelectric_point": 4.6600244522094725 }, "id": "NKOCPLAKPK_1046", "locus": "GCA003260355_01010" }, { "type": "cds", "sequence": "contig_1", "start": 550767, "stop": 550883, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTATCAAAGGGATTAAAATTCTGAATGAAACTGATGATCAGGTGCTTGTTCAAGTAGGAGCTGGCGAAGATCGAGATGACTTCGTTCGCTATAGTATTGAAAAGAGATGGTGA", "aa": "MAIKGIKILNETDDQVLVQVGAGEDRDDFVRYSIEKRW", "aa_hexdigest": "7f3e6e9915c7a3e18bd5b9ae8318fd4c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4408.942699999999, "isoelectric_point": 4.716181373596191 }, "id": "NKOCPLAKPK_1047", "locus": "GCA003260355_01011" }, { "type": "cds", "sequence": "contig_1", "start": 550938, "stop": 551084, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGAAAAATATTGATCTCAAACCTTATAACACGCTGCAAGTCGTCTGCAAAGCCAAATACTTCGTCGAAATAGAGAGTACCTATCAATTACTCCAACTCTTAGAAACGGCTGAGTGGAACGCAGAAAAGCATTGGATTCTTTGA", "aa": "MKKNIDLKPYNTLQVVCKAKYFVEIESTYQLLQLLETAEWNAEKHWIL", "aa_hexdigest": "d0c707114d183a82c135b6d0baefe491", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5784.7001999999975, "isoelectric_point": 6.536279487609863 }, "id": "NKOCPLAKPK_1048", "locus": "GCA003260355_01012" }, { "type": "cds", "sequence": "contig_1", "start": 551127, "stop": 551534, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02872.23" ], "nt": "ATGTATCCCTACGGAGGAGGAATATACGCACTAACCTTAACTGGAGAGGAGCTCAAGGAGATGATTCACTTTATTTTTAGATCCAGATTCTGTGGAGGAGGGGAGGAGTTCTTTGCATGGAGCAAGGGCGTTTCTATTGTTTATGACTGGAAAAACAAGGAAGTAAAGAGCATCCTTTATCAAGGACAGCCGCTTCAGGACAATAAAAACTATATCGTGGGAATCCAAAAATTCCATTTTGATAATATCGAACAAAATCTAGGCATCCATCTTAACCAGTTGAAATCAGGAAAATCTCCAAGACTTGTTTGTACGCAGGATTTTGATATTCTGGAAGAATACTTTCAGTCGCACCAGGAAATTACCGCAGAAATTGAGGGAAGGCTGATTTTTGAGAATCAAGAATAA", "aa": "MYPYGGGIYALTLTGEELKEMIHFIFRSRFCGGGEEFFAWSKGVSIVYDWKNKEVKSILYQGQPLQDNKNYIVGIQKFHFDNIEQNLGIHLNQLKSGKSPRLVCTQDFDILEEYFQSHQEITAEIEGRLIFENQE", "aa_hexdigest": "3602811fd4b6a7faab0726f7778f3607", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02872.23", "name": "5_nucleotid_C", "length": 85, "aa_cov": 0.5925925925925926, "hmm_cov": 0.9764705882352941, "evalue": 2.3147349806366054e-10, "score": 37.245059967041016, "start": 1, "stop": 80 } ], "seq_stats": { "molecular_weight": 15734.59749999999, "isoelectric_point": 5.218865394592284 }, "id": "NKOCPLAKPK_1049", "locus": "GCA003260355_01013" }, { "type": "cds", "sequence": "contig_1", "start": 551547, "stop": 551702, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTCAGGACCTAACACATCCAACAAGAACTGAGGAAACTCAAGTGAGTAACCTCATTGCAGATATTTTCAGAGAACAGCTTGGTTTGGATATGATGTGTTTGGGGACAGGATCGCTGAGAAATGAACTCTTAGAAAGTATCGTTACGCTTTGA", "aa": "MAQDLTHPTRTEETQVSNLIADIFREQLGLDMMCLGTGSLRNELLESIVTL", "aa_hexdigest": "310aeb53d367dfe5a981801b8692f36c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5705.474799999997, "isoelectric_point": 4.328709983825685 }, "id": "NKOCPLAKPK_1050", "locus": "GCA003260355_01014" }, { "type": "cds", "sequence": "contig_1", "start": 551868, "stop": 552497, "strand": "-", "frame": 2, "gene": null, "product": "Bifunctional metallophosphatase/5'-nucleotidase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7X6WDV7", "UniRef:UniRef90_A0A328EKA7" ], "nt": "ATGCTCTCTGGCTATATTCAGAAAGTTAAAAAAGAGGAAAAAAACTGTCTTTATACGATTTCTGGAGATATGCTCCAAGGATCTGTCGTGGATTCAGAGTTTAAGGGGATTTCAACAATTGATATCATGAATATGCTGAATCCAGACGTCGTTACCCTCGGAAACCACGAGATCGACTACGGATTAGCACACTTACTCTTCCTAGAAAAAATTGCAAAATTCCCGATTATAAACGCTAATCTTTACATAAGAACAACGAAAACAAGGCTTTTTAAGCCCTATTATATCAAGGAAATTGATGGGATGAAAATCCTTTTCATTGGAATCATTACAGAAGATGCTTTGAGTAAAACGACTTCTGATGCTCTGATTTCTACCTTTGTGAATGTTGAGGAAGCAGCGAGAGAGGTAGAGAGAATCTGTAATGCCTACAAAAGAATTGATATTGATTTCACGATACTTCTCACGCATATTGGATATGAAAATGATCTCAAACTCGCTGAACTCCTTGATCCAGAGCTTGGAGTCGACGTAATCATTGGTGGGCATTCTCATACTTTGCTAGAGCAGCCGACAAAGGTGAAGGATATCCTTGTTACTCAGGTTGGTGTCGGGACAAATCAGATTTGA", "aa": "MLSGYIQKVKKEEKNCLYTISGDMLQGSVVDSEFKGISTIDIMNMLNPDVVTLGNHEIDYGLAHLLFLEKIAKFPIINANLYIRTTKTRLFKPYYIKEIDGMKILFIGIITEDALSKTTSDALISTFVNVEEAAREVERICNAYKRIDIDFTILLTHIGYENDLKLAELLDPELGVDVIIGGHSHTLLEQPTKVKDILVTQVGVGTNQI", "aa_hexdigest": "39540ac03f7e5ece15c9cf7c9e3ffc58", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00307F728B", "query_cov": 1.0, "subject_cov": 0.842741935483871, "identity": 0.679, "score": 302.0, "evalue": 1.34e-101, "valid": false, "product": "metallophosphatase", "uniref50_id": "UniRef50_A0A497PF25" }, "genes": [], "truncated": "both", "pseudogene": { "start": 551565, "stop": 552596, "observations": { "mutation": [ 551870, 552509 ], "stop": [ 551870, 552509 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EKA7", "query_cov": 1.0, "subject_cov": 0.4274028629856851, "identity": 1.0, "score": 412.0, "evalue": 1.49e-141, "gene_start": 1, "gene_end": 209, "reference_start": 34, "reference_end": 242, "reference_sequence": "MGNYKKLTILHSNDMHGDFFEETQNQALVGGVSMLSGYIQKVKKEEKNCLYTISGDMLQGSVVDSEFKGISTIDIMNMLNPDVVTLGNHEIDYGLAHLLFLEKIAKFPIINANLYIRTTKTRLFKPYYIKEIDGMKILFIGIITEDALSKTTSDALISTFVNVEEAAREVERICNAYKRIDIDFTILLTHIGYENDLKLAELLDPELGVDVIIGGHSHTLLEQPTKVKDILVTQVGVGTNQIGRFDIIVDTDNNCVESYTWQAIPIVDAYCPRDPKIEKILKGYKDQTDQKYNRILSKMAQDLTHPTRTEETQVSNLIADIFREQLGLDMMCLGTGSLRNELLESIVTLGELREMYPYGGGIYALTLTGEELKEMIHFIFRSRFCGGGEEFFAWSKGVSIIYDWKNKEVKSILYQGQPLQDNKNYIVGIQKFHFDNIEQNLGIHLNKLKSGKSPRLVCTQDFDILEEYFQSHQEITAEIEGRLIFENQE" }, "paralog": false, "identity": 0.9941690962099126, "score": 676.0, "evalue": 7.96e-248, "description": "Internal stop codon at 551870, 552509. Nonsense mutation around 551870, 552509.", "psc": { "uniref90_id": "UniRef90_A0A328EKA7", "product": "Bifunctional metallophosphatase/5'-nucleotidase", "uniref50_id": "UniRef50_A0A7X6WDV7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EKA7", "UniRef:UniRef50_A0A7X6WDV7" ] }, "pscc": { "uniref50_id": "UniRef50_A0A7X6WDV7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7X6WDV7" ], "product": "Bifunctional metallophosphatase/5'-nucleotidase" } }, "id": "NKOCPLAKPK_1051", "locus": "GCA003260355_01015" }, { "type": "cds", "sequence": "contig_1", "start": 552759, "stop": 553649, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBF6AE1", "UniRef:UniRef100_A0A328EGT8", "UniRef:UniRef50_A0A328EGT8", "UniRef:UniRef90_A0A328EGT8" ], "nt": "ATGAAAAAGTTAGCACTTGCCCTAGTAGGGACGCTCAGTTTGATCGGATTTTCTCAGACGTCTGCCTATCTAGATTACAGCCCAAATTGTATACCAAATCCAGATTACCCATGTAATTACGACTTTGAATCCAACAATCAGGAGAATAAAACAACAACTCCAACAACTCCTACGACTCCTACTACGCCTCCAACTCAGCCAAGCAAGCCAACAACTCCAACCCAGCCAAACAAGCCAACAACTCCAACAAGTTCGACTACACCTTCTGTTCCTTCACAAAATCAGGGAAAAGAAGCAGGAGTATATATCTATTATGAAAGTGTAGAGGGGCATAATATCTATGCAGGTACGCTATACAAAGGTAAGGGAGGTGAGACGCTAGATCTCTCAAGCTTTATGAAGGAGCTCACCACTTTCGAATTCGATCACTATCGACCTGAACAGACCATTACTCTTGAATCAGGGAAATACTTGAGAGGAAAACTAATCTATCGCAAAAAGTTTAGAGAAGTGCAAAATACTCAGAACTACTCTCAACCTCAGAAACAAACAACTCAACCTTCTTTCTCTGATACCAATCAACGCACTTCTTCAGAGACGACAAAGCAGAACTCAACTTCTCAAGAACAGGTTTCAACTTCCCAATCTTCAACAAACCAATCTTCAGCTGAAACTAAATCTTCTAATACTGACGTAGAAAAACAAAAGATATTTACTGCTCTCTTTGACGATTACTTTAGAAGAAATAGTATGACCTCACTTGAAAAGCAGCTTTTCCTTACAGCTCTTAAAACAAATTTCCAAAATGCACAAAAGACTGTAGCTAAAGATCATCCTTATTATGCGACTTATAGTCTTATCATTTCAGCTATCGAAAATTATCGTTTCTAG", "aa": "MKKLALALVGTLSLIGFSQTSAYLDYSPNCIPNPDYPCNYDFESNNQENKTTTPTTPTTPTTPPTQPSKPTTPTQPNKPTTPTSSTTPSVPSQNQGKEAGVYIYYESVEGHNIYAGTLYKGKGGETLDLSSFMKELTTFEFDHYRPEQTITLESGKYLRGKLIYRKKFREVQNTQNYSQPQKQTTQPSFSDTNQRTSSETTKQNSTSQEQVSTSQSSTNQSSAETKSSNTDVEKQKIFTALFDDYFRRNSMTSLEKQLFLTALKTNFQNAQKTVAKDHPYYATYSLIISAIENYRF", "aa_hexdigest": "245af69b5f27546b90f3093a446a2bce", "start_type": "ATG", "rbs_motif": "TTTTT", "ups": { "uniparc_id": "UPI000DBF6AE1", "uniref100_id": "UniRef100_A0A328EGT8", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBF6AE1", "UniRef:UniRef100_A0A328EGT8" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EGT8", "uniref90_id": "UniRef90_A0A328EGT8" }, "psc": { "uniref90_id": "UniRef90_A0A328EGT8", "uniref50_id": "UniRef50_A0A328EGT8" }, "pscc": { "uniref50_id": "UniRef50_A0A328EGT8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EGT8" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 33326.35240000001, "isoelectric_point": 8.583659172058105 }, "id": "NKOCPLAKPK_1052", "locus": "GCA003260355_01016" }, { "type": "cds", "sequence": "contig_1", "start": 553794, "stop": 555116, "strand": "-", "frame": 2, "gene": "ftsH", "product": "ATP-dependent zinc metalloprotease FtsH", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A951TWZ8" ], "nt": "ATGGACGAGGTGAAAAACGAACTGATTGAAATCGTAGATTATCTCAAAGAGCCTGAAAAATACCAAAAAATCGGTGCCAGACCACCAAAGGGAGTATTGCTCTATGGAGCTCCAGGATCAGGAAAGACCTTGCTTGCCAGAGCGGTAGCAGGGGAGGCTGGAGTCGCTTTTTTCTCGGCTTCTGGGTCTGAATTTATGGAGATGTTAGTAGGTATGGGGGCTGCCAAGGTGAGAAAACTCTTTGAACAGGCCAAAGCTGCTGGAAAAGCGATTATTTTTATTGATGAAATTGATGCAATCGGGAAAAAGAGAGGAAGTGGCTATACCGGAGGACATCAGGAGCAGGAACAAACCCTCAACCAAATTCTCACCGAGATGGATGGTTTTGATAAAAATACAAATATTATTGTTATTGCAGCTACAAATAGGCCTGATACCTTGGATCCTGCCTTACTCAGAGCAGGAAGGTTTGATAGAAAAGTCTTTGTTTCCGTGCCAACATTAGAAGAAAGAATTCAGATTTTTGAATACTATTTGAAATCAAAGAAAGTAGATAAGAAGCTTAATCTTGAATCGCTTGCGAAGAGAACGAGCGGTTTAGTCGGTGCAGATATTGAAAATGTCGTGAATGAGGCGGCTTTACAGGTTGCAAAGTCCAATAGAGAAGTGCTAGAAGCGAAAGATTTTGAATACGCACTCGAGAAAGTCATTATGGGACCTGAGAAAAAAGTAAAATCTATGAAAGAAAAAGAGAAAAAGCTGGTTGCTTACCACGAGCTTGGACATGCAGTGACAGGACATCTCTTGGAAAACGCAGATCCGATTGAAAAAATCTCGATTGTTCGTAGAGGGCATGCTTTAGGAGTGACCTGGACGACTCCAGAAGAGGATAAAAACCTCTATTCCAAGGCGAAATTCCTTGATGAAGTGGTAACTCTGCTCGGAGGAAGGGCAGCGGAGGAGCTCTTCTTTGGAAAAGATGAGATCACTACTGGTGCATCTAATGATTTTGAGAGAGCGACTGATATTATCAAGAATATGATTCTCAAATACGGTATGGACGAGGATTTTGGACCTATAAACTATCTCAAAGAAGGAGAAGACCAGCCTTTTATCAATCCTTATAGTGAACAAACCGCACAGCTTATAGATGAAAAGATAAAAACTTATCTTGCCAATGCTTACAAAAAAGCAAAAAAAATTCTGAAGGAACACGACGTTTTAATCCATCAAATGGCGGGAATCCTTTTAGAGAAAGAATATCTTTCGAGTGAAGAGTTTACAGAAATGATGAAAGGTACAAACCCTGAAATAAAAGACTAA", "aa": "MDEVKNELIEIVDYLKEPEKYQKIGARPPKGVLLYGAPGSGKTLLARAVAGEAGVAFFSASGSEFMEMLVGMGAAKVRKLFEQAKAAGKAIIFIDEIDAIGKKRGSGYTGGHQEQEQTLNQILTEMDGFDKNTNIIVIAATNRPDTLDPALLRAGRFDRKVFVSVPTLEERIQIFEYYLKSKKVDKKLNLESLAKRTSGLVGADIENVVNEAALQVAKSNREVLEAKDFEYALEKVIMGPEKKVKSMKEKEKKLVAYHELGHAVTGHLLENADPIEKISIVRRGHALGVTWTTPEEDKNLYSKAKFLDEVVTLLGGRAAEELFFGKDEITTGASNDFERATDIIKNMILKYGMDEDFGPINYLKEGEDQPFINPYSEQTAQLIDEKIKTYLANAYKKAKKILKEHDVLIHQMAGILLEKEYLSSEEFTEMMKGTNPEIKD", "aa_hexdigest": "f99da8709f2ff6a5e50ff7914cc36d79", "start_type": "ATG", "rbs_motif": "TTTA", "psc": { "uniref90_id": "UniRef90_A0A2M7ULW8", "query_cov": 0.9840909090909091, "subject_cov": 0.8108614232209738, "identity": 0.527, "score": 440.0, "evalue": 5.7e-148, "valid": false, "gene": "ftsH", "product": "ATP-dependent zinc metalloprotease FtsH", "uniref50_id": "UniRef50_A0A951TWZ8", "go_ids": [ "GO:0004222", "GO:0006508", "GO:0016020", "GO:0016887", "GO:0051301" ] }, "pscc": { "uniref50_id": "UniRef50_A0A951TWZ8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A951TWZ8" ], "product": "ATP-dependent zinc metalloprotease FtsH" }, "genes": [ "ftsH" ], "id": "NKOCPLAKPK_1053", "locus": "GCA003260355_01017" }, { "type": "cds", "sequence": "contig_1", "start": 555180, "stop": 555707, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTAAAAGAAAAAAAATCAATTATAGGCTATAAGTGGCGAATTTATGGGATTCTTTTCATAATTCTAGTGTCTATTGGGCTGAGGCTAGTCCAAAATAATAGTCTGATACCTGCATTTGGTGTGGATACTGGAGTTATTCAGGTTATAGAAGATACAGAAAAAGTCTCACTCAACCAGTGGCTTGCAAACTATCATGACGGTGCTTATCAAAAAATTTCCCTGACAAATGACAAGGATCTAAAAGGGTTTTCTCTTCTCAAAAAAGCAAAAGAAAGCCAGCTCTTTGGTAGAGAGTATGAAACAGAAGTCTATAGGGCTTATCAAACAGAAAAGTCAGCTTCGCTCAATCTTATTGATCTTGGAGTAGCTTTGACTGGAGATATTGAGGTTGCTATCGTGCATGAGGAGCCTTCCCTTTGGAATAAATTGCTTGAACAGGTAGGCTATCTGATTTTTTTCCTTCTTATCCTCGTGCTTGGAATGAGGTTTATTATGCCAAAGGGCGGTCCAGGAAGCGTCTTATGA", "aa": "MVKEKKSIIGYKWRIYGILFIILVSIGLRLVQNNSLIPAFGVDTGVIQVIEDTEKVSLNQWLANYHDGAYQKISLTNDKDLKGFSLLKKAKESQLFGREYETEVYRAYQTEKSASLNLIDLGVALTGDIEVAIVHEEPSLWNKLLEQVGYLIFFLLILVLGMRFIMPKGGPGSVL", "aa_hexdigest": "7804cc7daff997d161cddd7123b166f4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19723.8373, "isoelectric_point": 6.744196510314942 }, "id": "NKOCPLAKPK_1054", "locus": "GCA003260355_01018" }, { "type": "cds", "sequence": "contig_1", "start": 556214, "stop": 556333, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACACACCTAAAAGTAACCTTGAACAGAAAAGTACAATCAATAAAATCCAGAGACTAGCTATGGCTTCAGCTCTTGTTATGGCTCTTCTTGCTGGTGCACCTGGATGTAAACAGTGA", "aa": "MNTPKSNLEQKSTINKIQRLAMASALVMALLAGAPGCKQ", "aa_hexdigest": "bd0b7d074539a2da98a5d2eddb940040", "start_type": "ATG", "rbs_motif": "TTCTT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4128.904799999999, "isoelectric_point": 10.032650566101072 }, "id": "NKOCPLAKPK_1055", "locus": "GCA003260355_01019" }, { "type": "cds", "sequence": "contig_1", "start": 556445, "stop": 556837, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EH24", "UniRef:UniRef90_A0A328EH24" ], "nt": "ATGGATGCCTCCAATCCTGCGGACATGATGAACAATTCAGCAGAAGGTGAAGAATGGTCAGAAGAAGACTTCGGAAAACTCATGGAAGTTGTTGATGTGGAAATTGAAAATGAATACTTAGAAGAAGAGAATAAGAAGCTTGATGAACAGAATAAGAAGCTTGATGAACAGAATAAGAAGCTTGATGAACAGAATAGAAAAGACCGTGCAGAAATATTAAAACGTAAACACGAAATAGAAAAATGGATCAATAGTCTTTCTGTAGAAGAACTACTTGCAGTCGATGGCATAGAAAAGGCTACTCATGCTTATATGGAAATTTGCAAAGGGTTGAACGAAACTCCAAACCCTAAAATGGTAACCGTTCAAAGAAAACTTCTTGCGAATCGTTAG", "aa": "MDASNPADMMNNSAEGEEWSEEDFGKLMEVVDVEIENEYLEEENKKLDEQNKKLDEQNKKLDEQNRKDRAEILKRKHEIEKWINSLSVEELLAVDGIEKATHAYMEICKGLNETPNPKMVTVQRKLLANR", "aa_hexdigest": "b019e23b00d8fe408d74702a1e867103", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 556214, "stop": 556837, "observations": { "mutation": [ 556331, 556442 ], "stop": [ 556331, 556442 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EH24", "query_cov": 1.0, "subject_cov": 0.6280193236714976, "identity": 1.0, "score": 196.0, "evalue": 1.98e-61, "gene_start": 1, "gene_end": 130, "reference_start": 78, "reference_end": 207, "reference_sequence": "MNTPKSNLEQKSTINKIQRLAMASALVMALLAGAPGCKQGAKNEKQTPQTEQTTETKDLIAHAAGNSSANTKDPSAGMDASNPADMMNNSAEGEEWSEEDFGKLMEVVDVEIENEYLEEENKKLDEQNKKLDEQNKKLDEQNRKDRAEILKRKHEIEKWINSLSVEELLAVDGIEKATHAYMEICKGLNETPNPKMVTVQRKLLANR" }, "paralog": false, "identity": 0.9903381642512077, "score": 343.0, "evalue": 3.96e-122, "description": "Internal stop codon at 556331, 556442. Nonsense mutation around 556331, 556442.", "psc": { "uniref90_id": "UniRef90_A0A328EH24", "uniref50_id": "UniRef50_A0A328EH24", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EH24", "UniRef:UniRef50_A0A328EH24" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EH24", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EH24" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 15176.84839999999, "isoelectric_point": 4.692763710021972 }, "id": "NKOCPLAKPK_1056", "locus": "GCA003260355_01020" }, { "type": "cds", "sequence": "contig_1", "start": 556867, "stop": 557178, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTATATTTGACGAAAAGGTCTCAGAATATAAAAAACCACTAATTAAACCCCATCAAGAAACAACATCCTCCATTTCAAAGTTTGTTGAAGAAAAGCTTGGGAAGGTTTCTCCTGCTGAGAAAAAAAGCATTTATGATCTCATCGAAAAGAAGTTAAAGAATAAGATTTCTCCAAAAAATATTATCGTAGTTCTCCAGCAATCTGAAGCCATTGCTAAGAGGAAAAATATCCCCTTTTTAGAGGTCTTACAAAAAGTCATTAGGCAAGTTGAGCAAAATTTAGCCCAGAAAAATGATATTAAAAACTGA", "aa": "MAIFDEKVSEYKKPLIKPHQETTSSISKFVEEKLGKVSPAEKKSIYDLIEKKLKNKISPKNIIVVLQQSEAIAKRKNIPFLEVLQKVIRQVEQNLAQKNDIKN", "aa_hexdigest": "41b4b3daf74d04249cc5481cca7bf875", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11872.82869999999, "isoelectric_point": 9.69825305938721 }, "id": "NKOCPLAKPK_1057", "locus": "GCA003260355_01021" }, { "type": "cds", "sequence": "contig_1", "start": 557212, "stop": 557508, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATAAGGGAGCTACGAGATCAAAGATTCAAGCTCAAGAATCAAAGATTCAAGCTCAAGAATCAAAAATAAAATTTCAAAACTCAAGTAAGGAAATTAAAAAGAGTTTGAAAGTTTTTGAACAAACAAAAGAAGCTTATCAAAAGCTTAATCCGAGTCTCAAGGAACATCATCCTGAAGAAGTCCAGCAGAAGCTAAAAGCCCTCTCTCCTGTCGTAAGGGCAAAGCTTAAGAAAAACAATATAGCTCCTGCTGAATATGCAAACTTCCTTCTCTCAAGAGAAAAGATTAGATGA", "aa": "MNKGATRSKIQAQESKIQAQESKIKFQNSSKEIKKSLKVFEQTKEAYQKLNPSLKEHHPEEVQQKLKALSPVVRAKLKKNNIAPAEYANFLLSREKIR", "aa_hexdigest": "963f68e28bb9d29c52d20648ab729744", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11303.967399999985, "isoelectric_point": 10.083064842224118 }, "id": "NKOCPLAKPK_1058", "locus": "GCA003260355_01022" }, { "type": "cds", "sequence": "contig_1", "start": 558027, "stop": 558491, "strand": "+", "frame": 3, "gene": null, "product": "DUF5667 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EK73", "UniRef:UniRef90_A0A328EK73" ], "nt": "ATGAAGAAATCTGTAAATCATTCTGTAGAAACAAAGAATGTCAATCCATCAAACCTACAAGATAAGCTAAGGAAGCTTACAACCAAGATCGCTCTTGCAACTGCTCTCTTGGTAGGATATAACTCTTGTGGGAAAAAACAACCTACATTGGAAGACAAACAAGAAGTAAAGATGGAAGTCATGGATACAAAACAAAAGAATGAAACTGGCAAGAACGAAAGTAATGCAATGGACACAGAAGTTAAAAATGTGGCTAATGATACTAAGAATGATCAACCTGAAGAGTTTAGCGATGAATGGTATGAAGCATTTGCTCTGTCGATAGCAGTTGATGAAGAATCTCAAAGACTCGATGAAGAATCCAAACAAATAGCTGAAGATAGGAAGAAAATAGCTGAAGATAGGAAGAAAATAGCTGAAGATACTGAAAATGCTATGCTTTCTATGGAAAGGAACCGAGAGTGA", "aa": "MKKSVNHSVETKNVNPSNLQDKLRKLTTKIALATALLVGYNSCGKKQPTLEDKQEVKMEVMDTKQKNETGKNESNAMDTEVKNVANDTKNDQPEEFSDEWYEAFALSIAVDEESQRLDEESKQIAEDRKKIAEDRKKIAEDTENAMLSMERNRE", "aa_hexdigest": "8f920a419ca95c86ea03caf05a28f9a5", "start_type": "ATG", "rbs_motif": "TTTTTA", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 558027, "stop": 558623, "observations": { "mutation": [ 558489 ], "stop": [ 558489 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EK73", "query_cov": 1.0, "subject_cov": 0.7777777777777778, "identity": 1.0, "score": 236.0, "evalue": 4.16e-77, "gene_start": 1, "gene_end": 154, "reference_start": 1, "reference_end": 154, "reference_sequence": "MKKSVNHSVETKNVNPSNLQDKLRKLTTKIALATALLVGYNSCGKKQPTLEDKQEVKMEVMDTKQKNETGKNESNAMDTEVKNVANDTKNDQPEEFSDEWYEAFALSIAVDEESQRLDEESKQIAEDRKKIAEDRKKIAEDTENAMLSMERNREGVDEDLLAFSSDLQTSLDEYFAACRELGREPSAHCKKLMASRKK" }, "paralog": false, "identity": 0.9949494949494949, "score": 332.0, "evalue": 1.75e-117, "description": "Internal stop codon at 558489. Nonsense mutation around 558489.", "psc": { "uniref90_id": "UniRef90_A0A328EK73", "product": "DUF5667 domain-containing protein", "uniref50_id": "UniRef50_A0A328EK73", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EK73", "UniRef:UniRef50_A0A328EK73" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EK73", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EK73" ], "product": "DUF5667 domain-containing protein" } }, "id": "NKOCPLAKPK_1059", "locus": "GCA003260355_01023" }, { "type": "cds", "sequence": "contig_1", "start": 558626, "stop": 559246, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAAAGAGAAACTCAGCATACATCCTGATACATCTGGAGAGATACAAAAAAAATCACTGAATCCTAATGAGGTAAAAGGCATTATTGACGTCGTTTTTGCTAACCTTTCTCCAGAAGAGAAGTCTTCTATAGGGAATGTAATTCTTGATTCTAAGCTCAAACTTTCTCTTAATAATATAAAGTTGATCCTTGAACAGGCAAAAAAAGGATCGGAAAAAACAAAAACCTCTCCATTAAATTTTGTGAGGAAGTTTTTGGACTTTCAAAAAAAACAAATGAAACAAAAACTTGATATACAAAAATTCCCTACTTTATCTGCTAAAGCACAGGACAATAAAAAAGAGAACCAAGTCAAAGAAAAAGAGAACCAAGTCAAAGAAAAAGAGAACCAAGTGAAACGAAAAAAAATTAATATAAATGAGCGTCTAACCCAGCAAATAAAGCAAACAAAAGAAGCTTATCAAAAACTTAATCCTACGCTTAGACAACATACCTCCGATGAACTTAACTCAAAAGTCTCTACTCTTTCTCCTGTCGTGAGGGCAAAACTTAAGAAAAACAATATCGCTCCTGCTGAATATGCAAACTTCCTTCTCTCAAGAGAAAAGATTAGATGA", "aa": "MNKEKLSIHPDTSGEIQKKSLNPNEVKGIIDVVFANLSPEEKSSIGNVILDSKLKLSLNNIKLILEQAKKGSEKTKTSPLNFVRKFLDFQKKQMKQKLDIQKFPTLSAKAQDNKKENQVKEKENQVKEKENQVKRKKININERLTQQIKQTKEAYQKLNPTLRQHTSDELNSKVSTLSPVVRAKLKKNNIAPAEYANFLLSREKIR", "aa_hexdigest": "75ecba398baeb601f62d122cdc52b214", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 23689.144100000023, "isoelectric_point": 9.974886894226074 }, "id": "NKOCPLAKPK_1060", "locus": "GCA003260355_01024" }, { "type": "cds", "sequence": "contig_1", "start": 559532, "stop": 559942, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGATGCAAAGCAAAAGCAAGGGGAAGCACTCTCCATACAAGAAAAGGAGATGATAACCGCATATCAGCAGCAACTTGAAGCACTCAAGGCACAGATGCAATCGAGGCTGACCAATTATCTCAAAGCTTCTATTACTCAAGCTCCGGTAGTTGCGCTGATGCGTTATCTTGGGCAAGAAAGTCTTGGAGGGAGGAAGCTCTCTGATCTCCTCTCTCATCAAGACAAACCTTTTGCTACGCTTGTCCAAGAGACGACGCTCACGGGAAAGGCTGAACCCATTCTCCAGCTCAAAGGAATGATTGAAGGGAAGCCGTTGACCTTTTATTACAATCTTGCTGATCCAACCGCTAGCTTGGAATGTGACGACAATCTTCACTATGATCCCTTTTCTCATCAGCTTTCTTTGTGA", "aa": "MDAKQKQGEALSIQEKEMITAYQQQLEALKAQMQSRLTNYLKASITQAPVVALMRYLGQESLGGRKLSDLLSHQDKPFATLVQETTLTGKAEPILQLKGMIEGKPLTFYYNLADPTASLECDDNLHYDPFSHQLSL", "aa_hexdigest": "e3cfe88c150683eeb5da830d57eb2292", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15231.25769999998, "isoelectric_point": 5.561661338806151 }, "id": "NKOCPLAKPK_1061", "locus": "GCA003260355_01025" }, { "type": "cds", "sequence": "contig_1", "start": 559982, "stop": 560095, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCAACGACCAAAGAGCTTTCTCAGCGTCTTGAAGAAGCATTGACACCAGAGAAGGTAAAAGATATTATTGATCAGGCTTCGGATCTTCAAGACTATCAGGAGAAAATCTGA", "aa": "MPTTKELSQRLEEALTPEKVKDIIDQASDLQDYQEKI", "aa_hexdigest": "060c553113565112e80d50b0478abfc0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4304.7825, "isoelectric_point": 4.399986076354981 }, "id": "NKOCPLAKPK_1062", "locus": "GCA003260355_01026" }, { "type": "cds", "sequence": "contig_1", "start": 560210, "stop": 560776, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCCTCCTGAGATCACGCACCAGCTGATGAAAGACGGCTTGATTAACCAGCACCGTCCTACGCAAAACGTATTTAGGCTTTTGGATCGCACGAGCGAAGAGAGCACAGACGCGGAACTCCAATCTTTTAGAATCGCGATAAAAAAGCTGGATTCCTTCCTCAAGTCTCCAAATCTCTCTCAAAAAATTATGGAAATTCAAGATCCTATCCTTCGTAATGTCCTCACAAAACTCTCTGATGCAAATCTAAAGAAATCTGACTTTCAAGTTCGATCGACTGCAATGCTAGATTTTTTTAACCTTTTCACCAGGCAGAATCTGACAGACAATAGCGTAAATCCTGAAGCTCCAGACTACAAGATGAATCTTTCAGACTTTTCTCGTTTTATTGGTTTTTTGGATCGTCCTGATCAGATGATTACCGATCCGAAACATTTGGAACATTTTTCACCAGCATTTCGTGAGAAGTATCAGCTCCAAGAGTCCGCTCAGCAGGGGGGGCTTTCCCACTTGGAGGATGAGATTGCAGCGGTCTATGAAGAGGAGCCATCCTACGCCTATGCATAG", "aa": "MPPEITHQLMKDGLINQHRPTQNVFRLLDRTSEESTDAELQSFRIAIKKLDSFLKSPNLSQKIMEIQDPILRNVLTKLSDANLKKSDFQVRSTAMLDFFNLFTRQNLTDNSVNPEAPDYKMNLSDFSRFIGFLDRPDQMITDPKHLEHFSPAFREKYQLQESAQQGGLSHLEDEIAAVYEEEPSYAYA", "aa_hexdigest": "ff8c43d2b687086fcced29a584a8e46c", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 21770.246099999982, "isoelectric_point": 5.125819969177246 }, "id": "NKOCPLAKPK_1063", "locus": "GCA003260355_01027" }, { "type": "cds", "sequence": "contig_1", "start": 560910, "stop": 561671, "strand": "-", "frame": 2, "gene": null, "product": "DUF306 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DC05300", "UniRef:UniRef100_A0A328EKU1", "UniRef:UniRef50_A0A328EKU1", "UniRef:UniRef90_A0A328EKU1" ], "nt": "ATGAAAAAAACACTTATGGCAGCAGGAATGCTTTGTGCTGCATCACTCGCAGTCAGCGGAATCTTCGCTGCAGAAATCAATCCTCTCGAGGCAACCGAATGGGAACTCACTTCTTTTGAAGGGAAAGCTTTGACGGGAGCAACAGTAAAGTTTGGGACAGGAAAGGTATTCGGATCTTTCTGCAATAATCTTTCTACGACCTATTCCCTTTCAGGAGACCAGATCCTAGCAGGACCAATGGCTACTACGCTCATGTACTGCAATACGCCTCTTATGGATGCTGAGAGCAAATTTGACCTTTCTAATGCTAAATATCAGCTTATGACAGGAAATCAACTCGAGCTTACCACAGCAAGCGGGTCAAAATACCTTTGGTCAAAAAAGACGACACATACAGTAGGAATGGCTAATCCTGCTTCTGTAAACTGTGAAAAGAATGGAGGAAAACATGAGATCGTAAAAGACAAAAACGGTAATGAATCTGGAGTATGTACGCTTTCTGATGGTACAAAATGTGATGAATGGGCATACTACAGAGGAGAATGTCCTGCTAAAGCAACTACTCAGGAGCAAAAGATTCATAAGATCCTTGATGATCACTTTGCAAAGCACGCCGATAAATTCCCAACTGCAAAGGAAAAAACTGCTGTTATCGAGATGCTCAAGACGACCTTTACTAATCTTATGAAAGAAGTAAACGCTACGCTCAAAGCCCAGTATCAAGAGATGGTAACTATCCTTGGGACTTATAAAGTAAAATAG", "aa": "MKKTLMAAGMLCAASLAVSGIFAAEINPLEATEWELTSFEGKALTGATVKFGTGKVFGSFCNNLSTTYSLSGDQILAGPMATTLMYCNTPLMDAESKFDLSNAKYQLMTGNQLELTTASGSKYLWSKKTTHTVGMANPASVNCEKNGGKHEIVKDKNGNESGVCTLSDGTKCDEWAYYRGECPAKATTQEQKIHKILDDHFAKHADKFPTAKEKTAVIEMLKTTFTNLMKEVNATLKAQYQEMVTILGTYKVK", "aa_hexdigest": "b1b3d1147f1115e75bbd6a0ca58ad131", "start_type": "ATG", "rbs_motif": "TTTTT", "ups": { "uniparc_id": "UPI000DC05300", "uniref100_id": "UniRef100_A0A328EKU1", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DC05300", "UniRef:UniRef100_A0A328EKU1" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EKU1", "uniref90_id": "UniRef90_A0A328EKU1" }, "psc": { "uniref90_id": "UniRef90_A0A328EKU1", "product": "DUF306 domain-containing protein", "uniref50_id": "UniRef50_A0A328EKU1" }, "pscc": { "uniref50_id": "UniRef50_A0A328EKU1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EKU1" ], "product": "DUF306 domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_1064", "locus": "GCA003260355_01028" }, { "type": "cds", "sequence": "contig_1", "start": 561857, "stop": 562021, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAATTCTTGTGCTGAATAGTGGTAGCTCTTCATTGAAATACCAGCTTTTTAATATGGAGAATCAAGAGGTTCTGGCTAAGGGACTTGTCGAGAGGATTGGGATTGACGGAGGGAAAATTAAGCATAAAACCGCAACTGGAGTAAAGCATGACATCGAATGA", "aa": "MKILVLNSGSSSLKYQLFNMENQEVLAKGLVERIGIDGGKIKHKTATGVKHDIE", "aa_hexdigest": "3866a38a7c4e6b1c48060aef622b057f", "start_type": "ATG", "rbs_motif": "TTATT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5939.8598999999995, "isoelectric_point": 9.158330345153807 }, "id": "NKOCPLAKPK_1065", "locus": "GCA003260355_01029" }, { "type": "cds", "sequence": "contig_1", "start": 562046, "stop": 562486, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCACAAGGTACTTGAACTTTTGGTTGATCCTGAGCTTGGAGCTTTGGGAAGCCTGTCTGAGATCAATGCGATCGGACATAGAATGGTTCATGGAGGTGAAAGCTTTAAGCAATCAACGATTATTACTCCAGGAGTAAAGGAAGAATTTAGGAGATTAACAGCACTTGCTCCGCTTCATAATCCGGCTAACCTGATGGGAGTAGAAGCTTGTGAAAAGATTCTTCCTGGTGTGCCTAATGTTGCGGTCTTTGATACCGCGTTTCATCAGACTATGAAACCTGAGCATTATCTCTATGCACTTCCTTATAGATGGTATCAAGACTATAAAGTGAGAAGATATGGTTTTCACGGGACTTCTCATAAGTATGTAAGTCAAAGATTAGCTGAACTGATGGGAAGATCAGATTTAAAGGTTATTACCTGTCACGTGGGAAATTGA", "aa": "MHKVLELLVDPELGALGSLSEINAIGHRMVHGGESFKQSTIITPGVKEEFRRLTALAPLHNPANLMGVEACEKILPGVPNVAVFDTAFHQTMKPEHYLYALPYRWYQDYKVRRYGFHGTSHKYVSQRLAELMGRSDLKVITCHVGN", "aa_hexdigest": "027d9b0e858026ac4ff47f5054da6cd3", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16456.8333, "isoelectric_point": 8.555486488342286 }, "id": "NKOCPLAKPK_1066", "locus": "GCA003260355_01030" }, { "type": "cds", "sequence": "contig_1", "start": 562532, "stop": 563044, "strand": "+", "frame": 2, "gene": null, "product": "Acetate kinase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_R7F4X1" ], "nt": "ATGGGACTCACTCCTCTTGAAGGGGTGATCATGGGAACGAGGTGTGGAAATATTGATCCAGCTATTGTGCCTTTTATGATGAAGCAAGAAAATCTGAGCGTTGAACAGATTGATACGATAATGAATAAGGAATCTGGGATTCTCGGACTCTTTGGTAAGTCAAGCGATCATAGAGACGTTGAAGATGGCTACCTTGCAGGCAGCGAAAAAGAAACCATGATCTTGGAAATGTATACGAATGCGATTTTGAAATATATTGGTAGTTATGCCGCACTACTTGAGGGCGTTGATGCTATCGTATTTACAGCTGGAGTGCTAGAAAACTCTATCATTCAGAGAAAGCTTCTTGCTGAAAGGCTTGCTTGGCTTGGAGTGGATTTTGACGAAAGCTTAAATAACTTTAGAGGAGAAGAGAAAAAACTGACTAAAGAAGGTAGTAAGGTTCAACTTTGGGTGATTCCAACTGATGAAGAACTTATGATTGCAAAGGATACTTATGCTCTTACTAAATAG", "aa": "MGLTPLEGVIMGTRCGNIDPAIVPFMMKQENLSVEQIDTIMNKESGILGLFGKSSDHRDVEDGYLAGSEKETMILEMYTNAILKYIGSYAALLEGVDAIVFTAGVLENSIIQRKLLAERLAWLGVDFDESLNNFRGEEKKLTKEGSKVQLWVIPTDEELMIAKDTYALTK", "aa_hexdigest": "51308e10d0cd282cd5adcb3f2735677c", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_R7F4X1", "query_cov": 0.9705882352941176, "subject_cov": 0.9593023255813954, "identity": 0.5329999999999999, "score": 180.0, "evalue": 3.69e-55, "valid": false, "product": "Acetate kinase", "ec_ids": [ "2.7.2.1" ], "uniref50_id": "UniRef50_R7F4X1", "kegg_orthology_id": "K00925" }, "pscc": { "uniref50_id": "UniRef50_R7F4X1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_R7F4X1" ], "product": "Acetate kinase" }, "genes": [], "id": "NKOCPLAKPK_1067", "locus": "GCA003260355_01031" }, { "type": "cds", "sequence": "contig_1", "start": 563220, "stop": 563525, "strand": "+", "frame": 3, "gene": null, "product": "TPM domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1V5ZQ87" ], "nt": "ATGGGAATCTCGTTTGCGCACGAGATTGATCCGATGCATTTGCCGAGATTTGAGTATTTTGTGAATGATTATTCTCAAGTCCTCAGCCAAGGGGAATTTGAAGAGCTCAATCAACTTGCTGAGACTCTTGAAACGACGTCAGGATATCAGGTCGTAACGGTTCTTTTTCCTCATAGAGAAGGGAATGAGCTTTTTGATATTGCACTCAAAGCGTTTAATGAAAACGGAATTGGAGATAAAGACAGGAATGATGGACTTTTGCTAGCGATTGCAACTGAGGAAAAGAAGATCAGAATTATGGTTTGA", "aa": "MGISFAHEIDPMHLPRFEYFVNDYSQVLSQGEFEELNQLAETLETTSGYQVVTVLFPHREGNELFDIALKAFNENGIGDKDRNDGLLLAIATEEKKIRIMV", "aa_hexdigest": "eb3c2e9f088fef9d02a4c57180903faf", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A1V5ZQ87", "query_cov": 0.8910891089108911, "subject_cov": 1.0, "identity": 0.8, "score": 150.0, "evalue": 1.43e-45, "valid": false, "product": "TPM domain-containing protein", "uniref50_id": "UniRef50_A0A1V5ZQ87" }, "pscc": { "uniref50_id": "UniRef50_A0A1V5ZQ87", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1V5ZQ87" ], "product": "TPM domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_1068", "locus": "GCA003260355_01032" }, { "type": "cds", "sequence": "contig_1", "start": 563532, "stop": 563726, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAAGCTGAGATTCCTGATGCTCTCGCCAAAGCGATCATTGAAGCACTTATTAGACCTGAAGTAAATAGCGGAAACCTCTATCAAGGAATCAAGAATTTTTATAGTTTTTTTTCTGATCAAGAGATAAAAGCTCAGGTTAAGCAACTGCTTCCTGAGGAAGAAGATTCCAGCTCTCAGATGACCTTTCTCTGA", "aa": "MEAEIPDALAKAIIEALIRPEVNSGNLYQGIKNFYSFFSDQEIKAQVKQLLPEEEDSSSQMTFL", "aa_hexdigest": "4cae793f8cd598bef8cc65b0bba9bf2b", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7237.0883, "isoelectric_point": 4.286308097839356 }, "id": "NKOCPLAKPK_1069", "locus": "GCA003260355_01033" }, { "type": "cds", "sequence": "contig_1", "start": 563779, "stop": 563943, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGATCTTCTAGAGGTAGTTGGAGAAGCTCAAGTTCAAGTTCAAGTAGCTCTTCTAGCTGGGGAGGAAGTTCTGGAGGTGGAGGCTGATTCTCTGGAGGTGGAGGAAGTTCTGGAGGCTGAGGTGCTGGAGATTAACTTGCAGTTTGAGAGCAAAAAAGTATAA", "aa": "MDLLEVVGEAQVQVQVALLAGEEVLEVEADSLEVEEVLEAEVLEINLQFESKKV", "aa_hexdigest": "90987d3206fa03c4d4e3b148edcb19fc", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5953.669599999997, "isoelectric_point": 4.0500284194946286 }, "id": "NKOCPLAKPK_1070", "locus": "GCA003260355_01034" }, { "type": "cds", "sequence": "contig_1", "start": 563980, "stop": 564561, "strand": "+", "frame": 1, "gene": "lemA", "product": "LemA family protein", "db_xrefs": [ "KEGG:K03744", "SO:0001217", "UniParc:UPI000DBF9AD5", "UniRef:UniRef100_A0A328EGU6", "UniRef:UniRef50_A0A3N2NER9", "UniRef:UniRef90_A0A328EGU6" ], "nt": "ATGAAGAAAGGAACGCTTTGGGGAATCGTTATTGCTGTGATCTTGATCGGAATTTCTGTGATTGCAGGTCAGTATAATTCCCTTGTAAAACTTGAGGAAACCGTCAAAGCCCAACGAGGACAGGTAGAATCCGTCTATCAAAGGAGAGCCGATCTCATCCCTAATATCGTAGCTACTGTCAAGGGAGAAGCTGCTTTTGAGCAGGAGACGTTGAGACAAGTAGTAGAGGCGAGAGCTTCTGCGACACAGATGAAAATAGATGCAAATAATGCACAAGACTTTGTAAAATTCCAGGCAGCTCAGGGAGAACTCTCTCAGGCATTGGGAAGATTGATGATGCTTACTGAGAACTATCCTAGCTTAAAAGCCAACCAAGCCTTTAGCGATCTCAGAGTTCAGCTCGAAGGAAGTGAAAATAGAATTGCTGTAGAAAGGATGAACTACAATAAGGTTGTAGGAAAGTATAATATCAAGCTGAGAAGCTTCCCGACCAATCTTATTGCTAAGTGGCTCGGATTTGAGAGAGCAAACCTCTTTGAAGCAACAACGGGTAGCGAAGTTGCGCCTGTAGTAGCATTCTAG", "aa": "MKKGTLWGIVIAVILIGISVIAGQYNSLVKLEETVKAQRGQVESVYQRRADLIPNIVATVKGEAAFEQETLRQVVEARASATQMKIDANNAQDFVKFQAAQGELSQALGRLMMLTENYPSLKANQAFSDLRVQLEGSENRIAVERMNYNKVVGKYNIKLRSFPTNLIAKWLGFERANLFEATTGSEVAPVVAF", "aa_hexdigest": "76b435dcbdd19301f43426ae9232b31e", "start_type": "ATG", "rbs_motif": "TTTTT", "ups": { "uniparc_id": "UPI000DBF9AD5", "uniref100_id": "UniRef100_A0A328EGU6", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBF9AD5", "UniRef:UniRef100_A0A328EGU6" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EGU6", "uniref90_id": "UniRef90_A0A328EGU6" }, "psc": { "uniref90_id": "UniRef90_A0A328EGU6", "product": "LemA family protein", "uniref50_id": "UniRef50_A0A3N2NER9", "kegg_orthology_id": "K03744" }, "pscc": { "uniref50_id": "UniRef50_A0A3N2NER9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3N2NER9" ], "product": "LemA family protein" }, "genes": [ "lemA" ], "id": "NKOCPLAKPK_1071", "locus": "GCA003260355_01035" }, { "type": "cds", "sequence": "contig_1", "start": 564886, "stop": 565056, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAGACTATTGTTTGTGAAAACAAGAGTTTCCGAGAGCAGGTAGAGAATCTCTGCCTGCTTTTTCGTTTTTTACAAAAAAAGACAGCCTGGAGGGACTGTCCTCATATATTTAAGGATTTCACACAAAGACATCATGAAGTCTTAGAGAAGAACGATATTTTCTTTTAG", "aa": "METIVCENKSFREQVENLCLLFRFLQKKTAWRDCPHIFKDFTQRHHEVLEKNDIFF", "aa_hexdigest": "188d721294c3990fa926062e210459ee", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6959.966199999999, "isoelectric_point": 6.812460136413574 }, "id": "NKOCPLAKPK_1072", "locus": "GCA003260355_01036" }, { "type": "cds", "sequence": "contig_1", "start": 565087, "stop": 565440, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4IHI9", "UniRef:UniRef90_A0A660MP06" ], "nt": "ATGAACGAATCAATGAAATTTTCTAATTCGCTTACCAAAACGAATACACTTCTCGAAAAAATTGCAACAACTTGTGATGCTCTGATTTCTGCATTAGAATACACTACAGAGGCGGCATGTCAATACGGAGGGGGAATCCTTCATCATTTTGAAAGTGAGTATGTAGATGTAGTAGTAAGCACGCTTCTCCAGTTGAAAGCTCACATCGCCAGATTAGCGAAAGAGCTAGATAATGGGTTGATTCAATCCACTAAAGATCTCCTTTTACTTGTCGAAGGGATGCAAGATATTCAGGACAAGCTGACAATGCTCCTTAGAGAAAATGGAAATCTGAGAAGTAATTTTGCATTCTGA", "aa": "MNESMKFSNSLTKTNTLLEKIATTCDALISALEYTTEAACQYGGGILHHFESEYVDVVVSTLLQLKAHIARLAKELDNGLIQSTKDLLLLVEGMQDIQDKLTMLLRENGNLRSNFAF", "aa_hexdigest": "a58615971a806c9c3ed75d8ddd30d651", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A660MP06", "query_cov": 1.0, "subject_cov": 0.8602941176470589, "identity": 1.0, "score": 224.0, "evalue": 8.86e-74, "valid": true, "uniref50_id": "UniRef50_A0A2W4IHI9" }, "pscc": { "uniref50_id": "UniRef50_A0A2W4IHI9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4IHI9" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13023.814499999982, "isoelectric_point": 5.0608530044555655 }, "id": "NKOCPLAKPK_1073", "locus": "GCA003260355_01037" }, { "type": "cds", "sequence": "contig_1", "start": 565680, "stop": 565892, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCTAAAAAATCTAAAAATCAGACTAATAATCCCAGAAAGTGCTATGACCAAGAGGCTCTTTAGCTATCTAAGGGTAAAAACCGGCTTCAGAAAATATACGATGACTTTGACTCCAATTCGCCTAGGCTCTCGAGAAAAAGCGAAAGTAATGTCTGGGGGGATCCTCTTTGAAGAGGTGGACCAGCATCTCGAGCTCAAAAAGCTTCCTTGA", "aa": "MLKNLKIRLIIPESAMTKRLFSYLRVKTGFRKYTMTLTPIRLGSREKAKVMSGGILFEEVDQHLELKKLP", "aa_hexdigest": "511efdd8dce6456765d4c5703c177ab5", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8164.810399999998, "isoelectric_point": 10.397412681579588 }, "id": "NKOCPLAKPK_1074", "locus": "GCA003260355_01038" }, { "type": "cds", "sequence": "contig_1", "start": 566001, "stop": 566411, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03486.20" ], "nt": "ATGATAAACTTTCTTAGAGGCCACGGAATCGAAAGCAAAGAGGAAGACGCTGGAAGAATCCTCCTAGAGAGCAATAAATCTCGTCAACTCGTGGATTTTCTGATCAAAGAGAATGAGAAAAATGCTGCAACAATCCTCACCAAGACGGAAGTCCTCCAGGTAGAGAGAATAGCAGAAGGTTTTTCCGTCAAAACATCAAATGAAACTTTCACCACTAAAAGGATAATTCTGGCTAGTGGAGGCTGTTCATTTCCGCAGATCGGAGGAGGAAAGTTTGCCTTTACTCTAGCAAAGGAGTACAAAATCGCCACAAAAGCTGCTCATCCTGCCCTCTGTGGAATCCAAACGCAGGAAAAGATCGACCAACTTTCCGGAAGTAGCACGTTTGCAAAAATTCAGCTTCTTGACTGA", "aa": "MINFLRGHGIESKEEDAGRILLESNKSRQLVDFLIKENEKNAATILTKTEVLQVERIAEGFSVKTSNETFTTKRIILASGGCSFPQIGGGKFAFTLAKEYKIATKAAHPALCGIQTQEKIDQLSGSSTFAKIQLLD", "aa_hexdigest": "93897833b8dbc83d5a490c304cb1dcb7", "start_type": "ATG", "rbs_motif": "TTTTT", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03486.20", "name": "HI0933_like", "length": 120, "aa_cov": 0.8676470588235294, "hmm_cov": 0.9833333333333333, "evalue": 1.094290785687751e-20, "score": 70.56782531738281, "start": 1, "stop": 118 } ], "seq_stats": { "molecular_weight": 14890.909699999978, "isoelectric_point": 8.563931846618651 }, "id": "NKOCPLAKPK_1075", "locus": "GCA003260355_01039" }, { "type": "cds", "sequence": "contig_1", "start": 566421, "stop": 566594, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTATTGCCAAAACAGCGAAAAAAGCTGATCCTAGAAAAGACAGACAGTATAGCAACTAAGCTCTTGCTTTCTGGAAAGGGGAGGTGTAATTTCACAAATCTTAATACTAGCTCAGATCATTATCTTGGAGATCAAAGAGAGAGACTCTCTGTTTTTTTTCAAGCTTTTTGA", "aa": "MLLPKQRKKLILEKTDSIATKLLLSGKGRCNFTNLNTSSDHYLGDQRERLSVFFQAF", "aa_hexdigest": "ec9aaca3cb406a139c0eb7052f761631", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6572.5733, "isoelectric_point": 9.895977592468263 }, "id": "NKOCPLAKPK_1076", "locus": "GCA003260355_01040" }, { "type": "cds", "sequence": "contig_1", "start": 566692, "stop": 567312, "strand": "-", "frame": 1, "gene": null, "product": "Phosphatidic acid phosphatase type 2/haloperoxidase domain-containing protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBF3BFF", "UniRef:UniRef100_A0A328EH36", "UniRef:UniRef50_A0A328EH36", "UniRef:UniRef90_A0A328EH36" ], "nt": "ATGTTTGATATCAGTGCAAAAAAACTTATCTATCTGAGTATTACTCTAGGTTTTTTCGTTTTGTTGGCAGCGCTTGGAATACTTCTTTTCCCTGAAGCAATCACAAAATGGGATCGTCGTCTTGCTCGCAAAAAAAACGATACTTCATGGAAACTTCAGCTTTCTCTTTTCATTAATCATGCAATTACTCCCTTATCAGTCGGAATATTCTATCTTCTAATGAGCGGAATCCTCATCTGGAGCAAAGAATTGAGAATTTTTGGAATCGGAGTAAGTAGTATTTTGGTCGCATCAATTGCCTTCCGAAGCGTAAAAAGAATAACTCAAAGACCAAGACCTGAAAAAGCAAAAATCACCTTCAAAGACTATAGTTTCCCAAGCGGACATACCACAGCAGGATTCGTATTTTTCTTGAGTGCAGCAATTGCTGTTTCTCGGCTCCTCAACCTTGAATATCGTGAAGTAATCTTTGTCTTTGCGATCATCTGCGGGGGAATTGTTGCTTGGAGTAGGCGATATCTCCATGTTCATCGATTTACTGATGTCGTTATAGGAGCACTGCTCGGAAGTGGGTGTTTCCTATTTTCGTATCTGATTTTTTCTATTTTGGTGATGCAATAA", "aa": "MFDISAKKLIYLSITLGFFVLLAALGILLFPEAITKWDRRLARKKNDTSWKLQLSLFINHAITPLSVGIFYLLMSGILIWSKELRIFGIGVSSILVASIAFRSVKRITQRPRPEKAKITFKDYSFPSGHTTAGFVFFLSAAIAVSRLLNLEYREVIFVFAIICGGIVAWSRRYLHVHRFTDVVIGALLGSGCFLFSYLIFSILVMQ", "aa_hexdigest": "90665fce94574de949165008a8462dfe", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000DBF3BFF", "uniref100_id": "UniRef100_A0A328EH36", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBF3BFF", "UniRef:UniRef100_A0A328EH36" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EH36", "uniref90_id": "UniRef90_A0A328EH36" }, "psc": { "uniref90_id": "UniRef90_A0A328EH36", "product": "Phosphatidic acid phosphatase type 2/haloperoxidase domain-containing protein", "uniref50_id": "UniRef50_A0A328EH36" }, "pscc": { "uniref50_id": "UniRef50_A0A328EH36", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EH36" ], "product": "Phosphatidic acid phosphatase type 2/haloperoxidase domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_1077", "locus": "GCA003260355_01041" }, { "type": "cds", "sequence": "contig_1", "start": 567466, "stop": 567660, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCTTGTCTTCCCTGGACTATTCAAAGGCTTATTAGAGGCAAGAATCCCTAAAGTAGAAAAAAAACATAAGCTTGCAGCAGCTCAAGCCCTTGCTAATTACCTCAAAAATCCAACCAGAGATGAAATACTACCTTCAGCCTTGGACAAACAGGTGGCAACTGTCATCGCAGATGCCGTTAAAGCCGTAGCCTAG", "aa": "MLVFPGLFKGLLEARIPKVEKKHKLAAAQALANYLKNPTRDEILPSALDKQVATVIADAVKAVA", "aa_hexdigest": "2838d2a7c38d6ef7fc51587e64a7cb8c", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6896.149500000003, "isoelectric_point": 9.69728603363037 }, "id": "NKOCPLAKPK_1078", "locus": "GCA003260355_01042" }, { "type": "cds", "sequence": "contig_1", "start": 567715, "stop": 567831, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAGCAAGCCAAATCTCATCTCTACTGAGGATGTAAAAAAGATGAATTCAGATCCTATTTTATTTGCTCTTTCTAATCCAGAGCCAGAGATTACCCCTGATGAGGCGAAAAAATGA", "aa": "MSKPNLISTEDVKKMNSDPILFALSNPEPEITPDEAKK", "aa_hexdigest": "c5cd35bbd8ae6a2dab2381aa09cda79c", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4228.794000000002, "isoelectric_point": 4.715101432800293 }, "id": "NKOCPLAKPK_1079", "locus": "GCA003260355_01043" }, { "type": "cds", "sequence": "contig_1", "start": 569090, "stop": 569437, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGAAAATTTTTCCTTACTTTCCACAGCAAGACTATAATTTCCAGCTTGTTTACAAACAATGCTTGAAAACTACAGAATCCATGCAAAGGAATCCTAATAAAGAAAATTTCTCAAGTTTCTATGAAAATTGTAAGAGTCCTCTTGCCAACATCACGACGATCATTCAAAATAAATATAGTGTAAAAGCAATTGCTAGTGCGAGTCCGATTAGTGGACCTGCGCCGCTAACTGTAACGTTTGACGCTAGAGCAAGTAGGGATCCTTCAAATGAAACGATTCCTGCTAGAAATTATTTTTGGTATTATAGGGATATTGATGGAATAGATAAACCCATTGGTTCTTGA", "aa": "MKKIFPYFPQQDYNFQLVYKQCLKTTESMQRNPNKENFSSFYENCKSPLANITTIIQNKYSVKAIASASPISGPAPLTVTFDARASRDPSNETIPARNYFWYYRDIDGIDKPIGS", "aa_hexdigest": "69e16490239fa3de103742f27075c446", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13203.769499999977, "isoelectric_point": 9.09727878570557 }, "id": "NKOCPLAKPK_1080", "locus": "GCA003260355_01044" }, { "type": "cds", "sequence": "contig_1", "start": 569441, "stop": 569623, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGCAAAACACACCTTCTCTGAGCCAGGAACCTATATTGTGCATCTGACGGTAAGGAGCTCTAATAGTGGAATTTTTGATGGTGAGGCAACGATATCCGTTAATGTAAGTCCAAAATCTGCAGTCATCAGCCTTTATGCTAATGGGAAAAAAATGCAGAAAAGTAAACCTTCTAAAATCTGA", "aa": "MAKHTFSEPGTYIVHLTVRSSNSGIFDGEATISVNVSPKSAVISLYANGKKMQKSKPSKI", "aa_hexdigest": "547d00c6fd4b19ac3282f193c4c2bf27", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6439.333300000002, "isoelectric_point": 9.901844215393066 }, "id": "NKOCPLAKPK_1081", "locus": "GCA003260355_01045" }, { "type": "cds", "sequence": "contig_1", "start": 569675, "stop": 569788, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGTGGGAGAGAGATTTTGGCATATAGACGAGATATTACGTCTAGAGATGGATTTAAATGGTCGAGAGAAGGCGATGGGAAACCTGGATATATCAATATTCCGCTTCCATGA", "aa": "MGGREILAYRRDITSRDGFKWSREGDGKPGYINIPLP", "aa_hexdigest": "904ecbef5ba8a5da4cd9d6fb0e7d57ff", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4222.7427, "isoelectric_point": 9.515162849426268 }, "id": "NKOCPLAKPK_1082", "locus": "GCA003260355_01046" }, { "type": "cds", "sequence": "contig_1", "start": 570020, "stop": 570481, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF18981.6", "PFAM:PF22352.2", "PFAM:PF00801.26", "PFAM:PF18911.6" ], "nt": "TTGCAAGGGAAAAGCATAAAAAAACAGTTTAAAAAGCCTGGAAACTATACTGTAAAACTCACCGTGGAGGATGAAATGGGACAGAAAAATAGTGATACGCTGAATGTTTATGTTGAGTCTACTCCTCCATCTCCTCAATTTTCTATTACCCCAACGAACAAACGACAGTATCCTTCAGAATTTTATCTTGATGCTAAAGCGTCTAACGATATAGATGTGAGTAATGGCTACGACCGTTTAACGTATGAATGGGTATTTTCTAATCCTAATGCCGTGCAAACTACCTCAACAGAAGAGAAAAACAAGAAAATAACCGTTCTTTTTAATGAAACGGGAAAGCACCTTGTTACCCTCAAAGTTTCCGATAATTATGGGAAAACTGAAGAAATCACAAAAGAAATCAATGTTGAATCTATTTTAAGACCAGAACTCACGATTCGTCCAAAATCTGCGGTTTGGTGA", "aa": "MQGKSIKKQFKKPGNYTVKLTVEDEMGQKNSDTLNVYVESTPPSPQFSITPTNKRQYPSEFYLDAKASNDIDVSNGYDRLTYEWVFSNPNAVQTTSTEEKNKKITVLFNETGKHLVTLKVSDNYGKTEEITKEINVESILRPELTIRPKSAVW", "aa_hexdigest": "58c14fc18ffce11d39b1623f7f078811", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF18981.6", "name": "InlK_D3", "length": 30, "aa_cov": 0.19607843137254902, "hmm_cov": 0.9666666666666667, "evalue": 2.9675012756107546e-07, "score": 26.943756103515625, "start": 9, "stop": 38 }, { "id": "PF22352.2", "name": "K319L-like_PKD", "length": 27, "aa_cov": 0.17647058823529413, "hmm_cov": 1.0, "evalue": 1.9750813818189955e-09, "score": 33.849246978759766, "start": 12, "stop": 38 }, { "id": "PF00801.26", "name": "PKD", "length": 55, "aa_cov": 0.35947712418300654, "hmm_cov": 0.9090909090909091, "evalue": 4.340152842613333e-15, "score": 51.96979904174805, "start": 77, "stop": 131 }, { "id": "PF18911.6", "name": "PKD_4", "length": 36, "aa_cov": 0.22875816993464052, "hmm_cov": 1.0, "evalue": 5.382323776722594e-17, "score": 58.13764953613281, "start": 2, "stop": 36 } ], "seq_stats": { "molecular_weight": 17457.3946, "isoelectric_point": 7.812101554870606 }, "id": "NKOCPLAKPK_1083", "locus": "GCA003260355_01047" }, { "type": "cds", "sequence": "contig_1", "start": 570590, "stop": 570787, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATATGAATATCAGAGAGTTGGTACCTACAATATCAAAGTAAAAGTGACTGATGAAAATGGAAACAGTAATGAAATCTATGATAAGGTTTTCATTGGAGAAAAAGACAGCCCTATTATCGGCTATGAAATCAAAGACACACGTTATTTTACTTTAGCTCAGAATGATGTTTGTCAGGTCCCTGATGAGAAGTGA", "aa": "MKYEYQRVGTYNIKVKVTDENGNSNEIYDKVFIGEKDSPIIGYEIKDTRYFTLAQNDVCQVPDEK", "aa_hexdigest": "8491179e24ea503dcb71eb0071be279a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7614.4004, "isoelectric_point": 4.7607431411743155 }, "id": "NKOCPLAKPK_1084", "locus": "GCA003260355_01048" }, { "type": "cds", "sequence": "contig_1", "start": 570836, "stop": 571108, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTACCATCAATACTGCACTTTCGGTTAATGCACAGGGAAATGCAAGTCAACTTAGGTATTACTTTCAAGCAAAGAATGATGAAATTGTAAAAAACCAGCAATTCTCGTATAAGTTTAATGGCGTAGGATGTCAGTATATTGACTATTCTCTTGAGGATACTTCACTGGGTAAAATCAGCAAAGAAAGAATATGGTTTAGAGTCGTTAATGCATTGCCAATCCTGGATAATCTGACGCTTAGTTTTCCTCAATATGGAAACGAAATCTGA", "aa": "MFTINTALSVNAQGNASQLRYYFQAKNDEIVKNQQFSYKFNGVGCQYIDYSLEDTSLGKISKERIWFRVVNALPILDNLTLSFPQYGNEI", "aa_hexdigest": "caba3a3949bdddb18c02f1d84b5f935b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10352.550199999992, "isoelectric_point": 6.121809577941893 }, "id": "NKOCPLAKPK_1085", "locus": "GCA003260355_01049" }, { "type": "cds", "sequence": "contig_1", "start": 571352, "stop": 571588, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A351Y537" ], "nt": "ATGTTTGATAATGATGATGGTTATCAGACAAGTGATGAACTTCTCGGGAATGGACCAATCATTATGTTCCCTCCTGATTCTAAACAGCCGGACATTCCGTTAGTTACACTCAGAGCTGATAAAATTAATGTAGAAGTCGGAGAAGAAGTTACCTTTGATATTATTTCAAAGATTCTCTCAGATAGATCTGATTTTGCTAAAGAAAGGACGATCCAGCTTGATTTTGATGGAGATTGA", "aa": "MFDNDDGYQTSDELLGNGPIIMFPPDSKQPDIPLVTLRADKINVEVGEEVTFDIISKILSDRSDFAKERTIQLDFDGD", "aa_hexdigest": "fc305650bd837a3b25a4e30aebe81dcc", "start_type": "ATG", "rbs_motif": "TTTA", "psc": { "uniref90_id": "UniRef90_A0A351Y537", "query_cov": 0.9871794871794872, "subject_cov": 0.875, "identity": 0.61, "score": 112.0, "evalue": 4.62e-31, "valid": false, "uniref50_id": "UniRef50_A0A351Y537" }, "pscc": { "uniref50_id": "UniRef50_A0A351Y537", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A351Y537" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8762.618199999994, "isoelectric_point": 4.0500284194946286 }, "id": "NKOCPLAKPK_1086", "locus": "GCA003260355_01050" }, { "type": "cds", "sequence": "contig_1", "start": 571703, "stop": 571804, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGCGAAAGTGCTCCTATCGTGGTGAAAAATGGAATAAAACCAGCATTGATCTATACTACATTAGGAACAACGACGCTTTTTAAAGATGTCAGCATTTGA", "aa": "MGESAPIVVKNGIKPALIYTTLGTTTLFKDVSI", "aa_hexdigest": "51cc212f1d807b8050d19d91fe1b12e5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3479.0896000000007, "isoelectric_point": 8.189692878723147 }, "id": "NKOCPLAKPK_1087", "locus": "GCA003260355_01051" }, { "type": "cds", "sequence": "contig_1", "start": 571791, "stop": 571979, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAGCATTTGAGAACTTATCGAGAGGAAAATTTGTCTTGATGTTTCAGAATGTGAAAAAGGAAATCCTGCCTATCTCAATACAACGCTCGATAAAACCTTTAAAGTACAATATCCAAAAAGTTGAAGTTACACGGTCCGCATTACTGCTAAGGATAAGAATGGGAACGAAGCAAGCAACACTCTGA", "aa": "MSAFENLSRGKFVLMFQNVKKEILPISIQRSIKPLKYNIQKVEVTRSALLLRIRMGTKQATL", "aa_hexdigest": "89dd391be22798327d51cc312bfc2b32", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7177.594400000001, "isoelectric_point": 11.112367057800292 }, "id": "NKOCPLAKPK_1088", "locus": "GCA003260355_01052" }, { "type": "cds", "sequence": "contig_1", "start": 572006, "stop": 572221, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATCCCCTTAGTTCTGGACTCTTTTTGATTTCTCTTCCTGAGGTTCAGTTTAGTTCTGATAAGATTCCTGAAATTTTTGTAGGGAAGCAGCTGAATAATGAAGTGCTTTTCTACCTCAAGAGTGATGAAAGTATAAAAAGTTGTTTCGTAGATAGCGATATTTCTTTTGATAGTAATCATGATGGAGATCCGAAAAATGATAGAGATTTTTGA", "aa": "MNPLSSGLFLISLPEVQFSSDKIPEIFVGKQLNNEVLFYLKSDESIKSCFVDSDISFDSNHDGDPKNDRDF", "aa_hexdigest": "ca161602d505d90b05d0a985f546773b", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8030.824699999996, "isoelectric_point": 4.260787391662597 }, "id": "NKOCPLAKPK_1089", "locus": "GCA003260355_01053" }, { "type": "cds", "sequence": "contig_1", "start": 572330, "stop": 572641, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTCAGTTTTGCTGACTTTGAGAAAGGTCTGGATGAGACGAGTAGAAAGCATTACCTTCTCGTTAGTGAACTTATCAATGGTATTGATGATAGCTCTAGTATCGCAAATTCTGACTTAAGAAGTCTTCTTATTATGCTTAGAAATAATTTGGGTGATAAGAATGCACAGAGAGGAAACATCATTCAGATTGAAGAATTTCTTGATAAAAACCAGCCTAAACCTAAATTATCTCAAAAACAGCAAGAAAAGCTGACTGATCTGATTAATACTCTTTCTGACTATGCAACACTTTCTGCGAAAGGAGTTTGA", "aa": "MVSFADFEKGLDETSRKHYLLVSELINGIDDSSSIANSDLRSLLIMLRNNLGDKNAQRGNIIQIEEFLDKNQPKPKLSQKQQEKLTDLINTLSDYATLSAKGV", "aa_hexdigest": "199e11210be4efdbfc4cef52f7aa270f", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11552.934599999986, "isoelectric_point": 5.315946388244629 }, "id": "NKOCPLAKPK_1090", "locus": "GCA003260355_01054" }, { "type": "cds", "sequence": "contig_1", "start": 572717, "stop": 573133, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAGTTTTGAATCCGGAGAAAGCGGTTCTGGAGAGTCTCTTCAGGACAAAAGGCAAAAAATGCTTGAAACTATCGCTGCTACGATTTATGCTTCAGTTGCTGAGTCTGATCAGCATATTGGAGAAAACGAAATCGCCAAGGAAGACTACCAAGATATCGTAAAACCCAACATTTGTAAAATTGCAAATGAATATCAGATTTCTACCCAAATGTGTAGTGATTTCAATAAAGATGACCCAAATTTTAAAGCGATTCCTGATGCTAAACAGCCAACTGGAAAAAAAACTGGCCTTCCTGGCCGATTGAAGGTTCTTCTTTGGATTGTAGGACTGGTTGTGCTTGGATTTATCGGACTGGTTACTACCTTTGCGATTAAAGCGAAACTCAGAGAAAGAATCGAAAATAATGAAGAATAA", "aa": "MSFESGESGSGESLQDKRQKMLETIAATIYASVAESDQHIGENEIAKEDYQDIVKPNICKIANEYQISTQMCSDFNKDDPNFKAIPDAKQPTGKKTGLPGRLKVLLWIVGLVVLGFIGLVTTFAIKAKLRERIENNEE", "aa_hexdigest": "aba77dc99bbce9b7355a4c85808b8466", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15307.246199999985, "isoelectric_point": 5.132811164855956 }, "id": "NKOCPLAKPK_1091", "locus": "GCA003260355_01055" }, { "type": "cds", "sequence": "contig_1", "start": 573954, "stop": 574616, "strand": "+", "frame": 3, "gene": null, "product": "Yip1 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4IJV9", "UniRef:UniRef90_A0A328EKV6" ], "nt": "ATGATTATAGTTTTCATCTATATTGTCTATGCTGCGATTCAGCTTATTGTAAGCGGAAAGGATGCAAATCAATTAAAAAATGCAATGACAAATCTCCTCTATGTATTGGTAGGATCGGCTCTTTTCTTTGGAGCGATGTGGCTTTTTGGAACAGTTCTCAACTTGAATGAAGTTAGGACGACGGCTGGCTTGAGGGATAATCTCATTTCTAGTAATGGAATTCTCTTCTTTGTTCTCAGCTTTTTGAAAGGAGCTGCGTTCTTTATTGCAATTATTATGATTGTTATCACTGGCTTTAGAATGATGAATCCTCAATCGGGAGAATCGGGAGATGGTAAAAAATTAGCAAAAAATCTGGTTAATGTGATCTTTGCTCTGGTAGCAATGAAGGTTATTGATTTTATCTATTATATTGCTAGTCAGCAAAACTTTGCATCGCAAGCAGGAAATTTTATCATTCAGGTTGCCAAGTTCCTCGCTTATGTGAGTGGAAGCGTAATAGTTTTGATGATTATTTACTCTGGATATCTCCTTGTCGTAGACGGAGGGAAAGGCGAGAACTTTAAGAAAGCAAAAAACACGCTGATCAATATTGTTTTTGCGGTGGCTGCACTGTTTTTCTTCCTCTTCATCCTTTACCAAATCTTCTCGGAATTCAACTAA", "aa": "MIIVFIYIVYAAIQLIVSGKDANQLKNAMTNLLYVLVGSALFFGAMWLFGTVLNLNEVRTTAGLRDNLISSNGILFFVLSFLKGAAFFIAIIMIVITGFRMMNPQSGESGDGKKLAKNLVNVIFALVAMKVIDFIYYIASQQNFASQAGNFIIQVAKFLAYVSGSVIVLMIIYSGYLLVVDGGKGENFKKAKNTLINIVFAVAALFFFLFILYQIFSEFN", "aa_hexdigest": "4828671b423863cf5f7d081ebfca2111", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 573666, "stop": 574616, "observations": { "mutation": [ 573687, 573894, 573918 ], "stop": [ 573687, 573894, 573918 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EKV6", "query_cov": 1.0, "subject_cov": 0.6962025316455697, "identity": 0.995, "score": 402.0, "evalue": 5.97e-140, "gene_start": 1, "gene_end": 220, "reference_start": 97, "reference_end": 316, "reference_sequence": "MFSRKKIGFLSLCSLFLLISGVSANSSPAPSFEADFAKKLTSDTADKRYGTEKVFSFSCVKKDQPFIDNVKCLFFPGVSPEGKAGGFLWEILRYAGMIIVFIYIIYAAIQLIVSGKDANQLKNAMTNLLYVLVGSALFFGAMWLFGTVLNLNEVRTTAGLRDNLISSNGILFFVLSFLKGAAFFIAIIMIVITGFRMMNPQSGESGDGKKLAKNLVNVIFALVAMKVIDFIYYIASQQNFASQAGNFIIQVAKFLAYVSGSVIVLMIIYSGYLLVVDGGKGENFKKAKNTLINIVFAVAALFFFLFILYQIFSEFN" }, "paralog": false, "identity": 0.9810126582278481, "score": 598.0, "evalue": 1.67e-219, "description": "Internal stop codon at 573687, 573894, 573918. Nonsense mutation around 573687, 573894, 573918.", "psc": { "uniref90_id": "UniRef90_A0A328EKV6", "product": "Yip1 domain-containing protein", "uniref50_id": "UniRef50_A0A2W4IJV9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EKV6", "UniRef:UniRef50_A0A2W4IJV9" ] }, "pscc": { "uniref50_id": "UniRef50_A0A2W4IJV9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4IJV9" ], "product": "EH domain-containing protein" } }, "id": "NKOCPLAKPK_1092", "locus": "GCA003260355_01056" }, { "type": "cds", "sequence": "contig_1", "start": 574745, "stop": 574858, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAAAACTCACAAAAGCTGATCGTATTCAGCAGATAGAAAAAGAGCTTGCAGAGCTCAAAGAGCGTTATCGTAGCAAAAAAGCTGAACGGGAAGCTCAAAAAGCTCAATGA", "aa": "MSKLTKADRIQQIEKELAELKERYRSKKAEREAQKAQ", "aa_hexdigest": "8e5a58cacab90c179f407870cb396681", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4433.057600000001, "isoelectric_point": 9.773100852966305 }, "id": "NKOCPLAKPK_1093", "locus": "GCA003260355_01057" }, { "type": "cds", "sequence": "contig_1", "start": 575114, "stop": 575263, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAGAAGTTAGCAGATTTGGAAAGCTTTTTGAGGAAGAGAGTTGTGGGACAGGATCAGGCTTTGCATCTGGTTGCTAATGCAATTCGTCGTGCAAGAGCTGGACTTAAAGATCCTAATAGACCAATTGGATCGTTTCTTTTCCTTTGA", "aa": "MEKLADLESFLRKRVVGQDQALHLVANAIRRARAGLKDPNRPIGSFLFL", "aa_hexdigest": "4029bbe00cbe36620eb0315baed23028", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5532.432199999998, "isoelectric_point": 10.834250450134277 }, "id": "NKOCPLAKPK_1094", "locus": "GCA003260355_01058" }, { "type": "cds", "sequence": "contig_1", "start": 575360, "stop": 575668, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF07724.20", "PFAM:PF07728.20" ], "nt": "ATGGAGAAACATGCGGTGGCAAGATTGATTGGTTCGCCTCCGGGATATATCGGGCATGAAGAAGGCGGACAGCTGACGGAAGCAGTAAGGAGAAAACCTTATGCAGTTGTGCTTTTTGATGAGGTTGAGAAGGCGCATCCAGAAGTTTTCAATCTTTTGCTCCAAGTATTAGATGAAGGAAGATTGACCGATAGTAAAGGTAAAACGGTGGATTTTAGGAATACGCTCATAATCCTAACTTCAAATATCGGTTCTCAGCTTCTTCTTGAAAAGATGAAAACTCAAGAGAAAAAGAAGTCTGAATCTTGA", "aa": "MEKHAVARLIGSPPGYIGHEEGGQLTEAVRRKPYAVVLFDEVEKAHPEVFNLLLQVLDEGRLTDSKGKTVDFRNTLIILTSNIGSQLLLEKMKTQEKKKSES", "aa_hexdigest": "782894b3daec5b781e513f10041b9096", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI000B555360", "query_cov": 0.8823529411764706, "subject_cov": 0.9375, "identity": 0.789, "score": 159.0, "evalue": 5.54e-49, "valid": false, "product": "AAA family ATPase", "uniref50_id": "UniRef50_A0A820Q4Q2" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07724.20", "name": "AAA_2", "length": 99, "aa_cov": 0.9705882352941176, "hmm_cov": 1.0, "evalue": 1.8664302537932502e-44, "score": 148.1018524169922, "start": 1, "stop": 99 }, { "id": "PF07728.20", "name": "AAA_5", "length": 70, "aa_cov": 0.6176470588235294, "hmm_cov": 0.9714285714285714, "evalue": 2.3449826800524907e-09, "score": 33.816680908203125, "start": 23, "stop": 85 } ], "seq_stats": { "molecular_weight": 11417.005399999987, "isoelectric_point": 6.838776588439941 }, "id": "NKOCPLAKPK_1095", "locus": "GCA003260355_01059" }, { "type": "cds", "sequence": "contig_1", "start": 575690, "stop": 576022, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF10431.15" ], "nt": "ATGATGCCTCTTTTACTTCAGCAGTTTAGGCCTGAATTTCTGAATAGAATTGATGATATTGTGCTATTTAATCCGTTAGCTCCTGAGCAAATTCGTGGCATTGTAGATATTCAGCTTTCTAACTACTTAAAGGAACTTTTAAGTGCTAGAGAGATTCAGCTTGAACTCGATAATGATGCGAAAAACTTCTTAGCAGAAAAAGGTTGGAATCCTGAATTTGGAGCCAGACCGCTCAAAAGAGCTTTACAGACGTATCTTTTAGATGAGCTTGCCTTGCAAATTATTGAGGGGAAGATAAAAAGCGGAAGTTTGGTAGCTGTCAGAAAAAAATGA", "aa": "MMPLLLQQFRPEFLNRIDDIVLFNPLAPEQIRGIVDIQLSNYLKELLSAREIQLELDNDAKNFLAEKGWNPEFGARPLKRALQTYLLDELALQIIEGKIKSGSLVAVRKK", "aa_hexdigest": "66833e24249bb48c21dfcbc7d141c513", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3C1IWD0", "query_cov": 0.9181818181818182, "subject_cov": 0.8938053097345132, "identity": 0.515, "score": 113.0, "evalue": 1.68e-30, "valid": false, "product": "Clp ATPase C-terminal domain-containing protein", "uniref50_id": "UniRef50_A0A292SK88" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF10431.15", "name": "ClpB_D2-small", "length": 82, "aa_cov": 0.7454545454545455, "hmm_cov": 0.9878048780487805, "evalue": 2.4501545361133866e-25, "score": 84.85031127929688, "start": 26, "stop": 107 } ], "seq_stats": { "molecular_weight": 12645.659699999984, "isoelectric_point": 6.288916206359863 }, "id": "NKOCPLAKPK_1096", "locus": "GCA003260355_01060" }, { "type": "cds", "sequence": "contig_1", "start": 576294, "stop": 576401, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCCAGCACTTGAGTATGTAGATTCTCTCGGGCTTTTAGTAAAAATAGATGGAATGCAGACGATTGATGCCATTTTTGCCCAGATTGAACAGCTCCTCCAAAATTAA", "aa": "MPALEYVDSLGLLVKIDGMQTIDAIFAQIEQLLQN", "aa_hexdigest": "23fa6f6b8239829969e0b18d817ac53f", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3891.5069000000003, "isoelectric_point": 4.0500284194946286 }, "id": "NKOCPLAKPK_1097", "locus": "GCA003260355_01061" }, { "type": "cds", "sequence": "contig_1", "start": 576507, "stop": 576938, "strand": "-", "frame": 2, "gene": null, "product": "Adenylate kinase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7W4E421", "UniRef:UniRef90_A0A328EKB8" ], "nt": "ATGAAAGACATTATCCTCGCTGGAATCCAGGCTTCAGGAAAGGGAACTCAAGCCCAGAAGCTTTTAGAAAAATACGGAAACCAGATTGCCTACTTTGAAACTGGAAACATTCTCAGAGCTTTGATGAGTAATGAAAATGTAATCGGGAACTACCTCAGGGAAACGGTAAATTCCGGTCGCCTTGTGAAAGATGAGATCGTCATAGGTCTTTTTAAAGTTTTTTTGGAAACCTTAGATGATAAAGGTCTTCTCGGTGATGGAAGCCTAAGAAGAATAGGTCAGACAAAAGGAATCCTTGATGCTTTATTAGAGAGGGGGAGAAAGCCACTTGTTATCCAGCTTGAGATCCCAGAAGAAGAAGTATACAAGAGACTTGACCAGCGTAAAATGTGTAAAAACTGTGGAACAATTCATTCTATGCTTATCGATTGA", "aa": "MKDIILAGIQASGKGTQAQKLLEKYGNQIAYFETGNILRALMSNENVIGNYLRETVNSGRLVKDEIVIGLFKVFLETLDDKGLLGDGSLRRIGQTKGILDALLERGRKPLVIQLEIPEEEVYKRLDQRKMCKNCGTIHSMLID", "aa_hexdigest": "9e0783ae6f933c43324b37111113cea3", "start_type": "ATG", "rbs_motif": "TTATT", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 576294, "stop": 576938, "observations": { "mutation": [ 576479, 576482, 576509 ], "stop": [ 576479, 576482, 576509 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EKB8", "query_cov": 1.0, "subject_cov": 0.6682242990654206, "identity": 0.986, "score": 275.0, "evalue": 1.92e-92, "gene_start": 1, "gene_end": 143, "reference_start": 1, "reference_end": 143, "reference_sequence": "MKDIILAGIQASGKGTQAQKLLEKYGNQIAYFETGNILRALMSNENVIGNYLRETVNSGRLVKDEIVIGLFKVFLETLDDKGLLGDGSLRRIGQTKGILDALLGRGRKPLVIQLEIPEEEVYKRLAQRKMCKNCGTIHSMLIDGEVEKCKKCGGELYVRADDADQSAIANRIAEYQKETVPALEYVASLGLLVKIDGMQTIDAIFAQIEQLLQN" }, "paralog": false, "identity": 0.9719626168224299, "score": 398.0, "evalue": 2.1e-143, "description": "Internal stop codon at 576479, 576482, 576509. Nonsense mutation around 576479, 576482, 576509.", "psc": { "uniref90_id": "UniRef90_A0A328EKB8", "product": "Adenylate kinase", "uniref50_id": "UniRef50_A0A7W4E421", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EKB8", "UniRef:UniRef50_A0A7W4E421" ] }, "pscc": { "uniref50_id": "UniRef50_A0A7W4E421", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7W4E421" ], "product": "Adenylate kinase" } }, "id": "NKOCPLAKPK_1098", "locus": "GCA003260355_01062" }, { "type": "cds", "sequence": "contig_1", "start": 577028, "stop": 577426, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EH05", "UniRef:UniRef90_A0A328EH05" ], "nt": "ATGTCAGGATTAACAGCAGAAATTTATAGAATTCCAGACGCCGAGGTCAAGCCACTTAACCAAAGGAAAGAAGCAGAAAAGACAACTATTGAACAAAAGAGTAATGAGCCCATAGATCCACAAGCGGAAAAGTGGACGAGAGCAGTAGATGCTTATTTTGGAAAAAATAAAAATACTGAACAAATGAAGGAAGATTTGGCTTCTCATCTGCAAAAACAAATAGAAGATCAAGCTATTATTAATGCAGAACAACAAAGAGAGATAGAAGAACTAAAAAAAGAACAGAAGAATCCAGATTCGATGAATAATTATAATCCTGAAGCACAAGGAAAATTTGTTAAGTGGAATAATAATATCGACAATCCAGATCATATTGAGGTTGGAAATGCTTCGGCTTGA", "aa": "MSGLTAEIYRIPDAEVKPLNQRKEAEKTTIEQKSNEPIDPQAEKWTRAVDAYFGKNKNTEQMKEDLASHLQKQIEDQAIINAEQQREIEELKKEQKNPDSMNNYNPEAQGKFVKWNNNIDNPDHIEVGNASA", "aa_hexdigest": "f42d788f9f3ccde8cf14f0e7aedfe4ef", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A328EH05", "query_cov": 1.0, "subject_cov": 0.9924812030075187, "identity": 1.0, "score": 256.0, "evalue": 1.62e-86, "valid": true, "uniref50_id": "UniRef50_A0A328EH05" }, "pscc": { "uniref50_id": "UniRef50_A0A328EH05", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EH05" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15179.559099999991, "isoelectric_point": 4.9329652786254865 }, "id": "NKOCPLAKPK_1099", "locus": "GCA003260355_01063" }, { "type": "cds", "sequence": "contig_1", "start": 577453, "stop": 577962, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBFE0A8", "UniRef:UniRef100_A0A328EGI4", "UniRef:UniRef50_A0A7W4ECJ8", "UniRef:UniRef90_A0A328EGI4" ], "nt": "ATGACCACAAACACTCAGCAGGTAGGGAATAAAGTCCCAAATCAACTACTCAATCAAGAAATCCTCAAACTTATCCAAGAACAAGGTTCAGGAACAGGACTTGAAGGACTCTTAAGTTTTGAGGAAAGACTAGAAAAAAGCGATAAAAAGCTGACTCCTTCCGAACTTACGACCGCTCTTGAACAGGTAAAAACTGACATCCAAAATCCAGACTTTCTTTCTTTTGAAAGCGGAAAAAGGGACGAAAAAGACTTCGGATTTGAAGGGGTGGGAACGGATGTAATTCCTACATTAACTCCGAATGGAGACTGTATTATCCCTGTGAACTTCCGATATGATGGGCAAAGGAGAACGATTCTTTTAATTCTCCATGTAAGTACTCAAGGAAAGATAAATATTTATCACCTACCAGAGAGCGATAATCGCTATACGATGAAAATCAATGATGTAGAATTTACCTATCAGCTTGTCGCTGAGGGGGAGAAATTTAAAATGCAGCTAGAGAAGTAG", "aa": "MTTNTQQVGNKVPNQLLNQEILKLIQEQGSGTGLEGLLSFEERLEKSDKKLTPSELTTALEQVKTDIQNPDFLSFESGKRDEKDFGFEGVGTDVIPTLTPNGDCIIPVNFRYDGQRRTILLILHVSTQGKINIYHLPESDNRYTMKINDVEFTYQLVAEGEKFKMQLEK", "aa_hexdigest": "238c47920b0ea139bfcd43783079865a", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000DBFE0A8", "uniref100_id": "UniRef100_A0A328EGI4", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBFE0A8", "UniRef:UniRef100_A0A328EGI4" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EGI4", "uniref90_id": "UniRef90_A0A328EGI4" }, "psc": { "uniref90_id": "UniRef90_A0A328EGI4", "uniref50_id": "UniRef50_A0A7W4ECJ8" }, "pscc": { "uniref50_id": "UniRef50_A0A7W4ECJ8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7W4ECJ8" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19181.449499999988, "isoelectric_point": 4.931316947937011 }, "id": "NKOCPLAKPK_1100", "locus": "GCA003260355_01064" }, { "type": "cds", "sequence": "contig_1", "start": 577987, "stop": 578265, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBF635B", "UniRef:UniRef100_A0A328EIQ9", "UniRef:UniRef50_A0A7W4HH96", "UniRef:UniRef90_A0A328EIQ9" ], "nt": "ATGACCAAAAAAGAGTTTATGCTCCAGATTATCAATTTCCTGCCGGATCGAAAGATGGGACGAGGGCTAAAAGCGCTCATTGAGAATAATCAGCTTTCTCCTGAGGTTTTTGAGAAGTTGTATGAAATTTTCAGACAAAGTATCCATACAACGTATACAGAGGTTCAGAAGGCTGAATTCAAAGAAAAACTCCAAAAGATTGAAGCTATGAAACAGCAAGAAGCTCAGAAGAGTGAGGAGGAGATCGTTGATTTAGATACAATGCTTGATGCTTTGTAA", "aa": "MTKKEFMLQIINFLPDRKMGRGLKALIENNQLSPEVFEKLYEIFRQSIHTTYTEVQKAEFKEKLQKIEAMKQQEAQKSEEEIVDLDTMLDAL", "aa_hexdigest": "43c04c6dbcceeeae27d32d9aca03aa8b", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000DBF635B", "uniref100_id": "UniRef100_A0A328EIQ9", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBF635B", "UniRef:UniRef100_A0A328EIQ9" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EIQ9", "uniref90_id": "UniRef90_A0A328EIQ9" }, "psc": { "uniref90_id": "UniRef90_A0A328EIQ9", "uniref50_id": "UniRef50_A0A7W4HH96" }, "pscc": { "uniref50_id": "UniRef50_A0A7W4HH96", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7W4HH96" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10909.521499999995, "isoelectric_point": 5.219320106506346 }, "id": "NKOCPLAKPK_1101", "locus": "GCA003260355_01065" }, { "type": "cds", "sequence": "contig_1", "start": 578400, "stop": 578942, "strand": "+", "frame": 3, "gene": null, "product": "Mur ligase central domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3D0UTE0" ], "nt": "ATGAAAACTCTGATTAAAAAGATCATTTGTTATGATAAACGATATCCTGTCTTCAAAAATTCAGTTTTTTATCATGTTTTTAAGAAAGCTCAGGCAAATATTGCCACTTCACTCTATAGCAGTCCCGCAAATGGATTTTTCATCATCGGAGTGACGGGAACTAACGGGAAAACGACGACGGTCAATTTGATTCATAAAATCTTAAATGATAATGTAGCTCCAACAGTCGCAGTAAGTACTGCCGATTTGAGAATTGGGAATAAAAAAATCACCAATACCAAGAAAATGACCTCTCTAGATGTTTTTGATCTCCAATCTCTCCTCTCTACTGCGAAAAATAATGGTTGCAAGATTGCGGTTTTAGAGGCTTCTTCTCAAGGATTGGATCAACAAAGATTTGCAGGTATTGACTTTGATATGGCAGTTTTGACCAATATTACACATGATCATTTGGATTATCATGGGACAATGGAAGCTTACGCTGCAAGCAAAAAATCACTTTTTAAGAATGTACTTTCCAATACTAAACAGAATAAATTCTGA", "aa": "MKTLIKKIICYDKRYPVFKNSVFYHVFKKAQANIATSLYSSPANGFFIIGVTGTNGKTTTVNLIHKILNDNVAPTVAVSTADLRIGNKKITNTKKMTSLDVFDLQSLLSTAKNNGCKIAVLEASSQGLDQQRFAGIDFDMAVLTNITHDHLDYHGTMEAYAASKKSLFKNVLSNTKQNKF", "aa_hexdigest": "21c42aae9ef28fc87e18eb02e3a69b7a", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3D0UTE0", "query_cov": 1.0, "subject_cov": 0.8780487804878049, "identity": 0.6609999999999999, "score": 241.0, "evalue": 1.54e-78, "valid": false, "product": "Mur ligase central domain-containing protein", "uniref50_id": "UniRef50_A0A3D0UTE0" }, "pscc": { "uniref50_id": "UniRef50_A0A3D0UTE0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3D0UTE0" ], "product": "Mur ligase central domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_1102", "locus": "GCA003260355_01066" }, { "type": "cds", "sequence": "contig_1", "start": 578988, "stop": 579098, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCGTTTGACAAGAAGATCAATTATTCTCGTCAGGCTTCTTCTATTTTGAAAGCAACTCAAGTCGTAGAATATCTTGATCATACAGAGTTTGTCTTTTCGTATCTTTGA", "aa": "MPFDKKINYSRQASSILKATQVVEYLDHTEFVFSYL", "aa_hexdigest": "816f23b007d825229da010cfcce8a194", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4269.826999999999, "isoelectric_point": 6.517522621154785 }, "id": "NKOCPLAKPK_1103", "locus": "GCA003260355_01067" }, { "type": "cds", "sequence": "contig_1", "start": 579585, "stop": 579719, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATATTGCACAACCTGGAGATATTGTGCTCTTGGCTGGTAAAGGACATGAACAAGTTCAACTTACTAATTTTGGGAAGAGAAAACGAAATGATAGGACTGCCCTACTAGAACTTGCAAGATCAGCGAGATAA", "aa": "MDIAQPGDIVLLAGKGHEQVQLTNFGKRKRNDRTALLELARSAR", "aa_hexdigest": "a5046f0fa6bff1889eea259f53207865", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4888.5706, "isoelectric_point": 10.24610538482666 }, "id": "NKOCPLAKPK_1104", "locus": "GCA003260355_01068" }, { "type": "cds", "sequence": "contig_1", "start": 579756, "stop": 580292, "strand": "-", "frame": 2, "gene": null, "product": "Primosomal protein N C-terminal domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3D0USJ9" ], "nt": "ATGCAAGGTTGGAAATTAGATTTGCTTGTGATTCTGGATGCAGATATCGGGCTTAATATCCCTGATTATAATGCAAGCGAGAAAAATTTTTACTTTCTTTATGAAGCATTCTGCAAACATCATTGTCAAAATTTCCTGGTTCAGACAAGGAATCCCGATCATCATAGTATCCGTAGTGCTTGTAAAATGCAAAAATCTGATTTTTTTGCTGTTGAAAACCTCTTTCGTAAGCAATATTCCTATCCACCATTTGGAGAGTTGTGCCTTATCCTCTACAAAGATGAAATCGAGCAAAAGCTTTTCAATAAGGTAGATCAGCTATACAAAGAGCTACTTTATCTTCAGCAGAAGTATCAGCTTGACGACCTAGAAATCTATACGACACCTCCATTAATTTACAAAATCTTTGGAAAATATAGGTATAATGTAGTCATTAAAGGCAGGGAAGTGAGGAATTTTATGGATATTGTTTACCCAAAACTCCAGCTCTCCAAGAAAGGCTTTAAAATCAATCGAATGGCTGAGAGTATGGTATAA", "aa": "MQGWKLDLLVILDADIGLNIPDYNASEKNFYFLYEAFCKHHCQNFLVQTRNPDHHSIRSACKMQKSDFFAVENLFRKQYSYPPFGELCLILYKDEIEQKLFNKVDQLYKELLYLQQKYQLDDLEIYTTPPLIYKIFGKYRYNVVIKGREVRNFMDIVYPKLQLSKKGFKINRMAESMV", "aa_hexdigest": "8622b992f7f0319aadc3a6a0fce613ff", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3D5YER8", "query_cov": 1.0, "subject_cov": 0.9468085106382979, "identity": 0.579, "score": 231.0, "evalue": 5.15e-75, "valid": false, "product": "Primosomal protein N C-terminal domain-containing protein", "uniref50_id": "UniRef50_A0A3D0USJ9" }, "pscc": { "uniref50_id": "UniRef50_A0A3D0USJ9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3D0USJ9" ], "product": "Primosomal protein N C-terminal domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_1105", "locus": "GCA003260355_01069" }, { "type": "cds", "sequence": "contig_1", "start": 580314, "stop": 580472, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGCACCCAAAAGCTCGCTCAGTTACTCAAAGAAGAATACGGAGCAGAGAGTCTGATTATTGAGTCCGAAACTGTAAGATCTCCCAATAAAATCCAAAAATTCAAGGCTCAACTCTCTGAATTTACTCAGCAAGGAAAAAATCCAATCCTGATTTGA", "aa": "MGTQKLAQLLKEEYGAESLIIESETVRSPNKIQKFKAQLSEFTQQGKNPILI", "aa_hexdigest": "32c2f2fe24ff72a2ae53ac05f4c76181", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5877.7195, "isoelectric_point": 8.065784645080566 }, "id": "NKOCPLAKPK_1106", "locus": "GCA003260355_01070" }, { "type": "cds", "sequence": "contig_1", "start": 580680, "stop": 580814, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACTACGAACTCTTTGATCATGGAGAAAAAAAAATCTACCTGGTAAATATGACCAAAGAGCTCAACCAGAATTTTTCTGAACTATTTGAAAAGGGGCTTGCAAAATACCTAAGGACCAACAAAAAAATCTGA", "aa": "MNYELFDHGEKKIYLVNMTKELNQNFSELFEKGLAKYLRTNKKI", "aa_hexdigest": "44581bec533a7bcce5c4f38ae825a446", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5340.1364, "isoelectric_point": 9.049958992004395 }, "id": "NKOCPLAKPK_1107", "locus": "GCA003260355_01071" }, { "type": "cds", "sequence": "contig_1", "start": 581949, "stop": 582200, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTCTTATATCTTATTCTTAGAACTCATTCTTACTCAAAGCAAGAAAATAAAAAAGATCTCGAGGAGCTAATTGAAAGTTTCTCTCCATCTGAAATAGTAAATAATTCTAAAATAGTAAATAATGAAGAAGCCTCTATTTTGAACTTAACCAAAAAATTCTTGAAAATTCCATCTAATATTTCTTGTGATGACAAAGTATTCATAGAAACATTATGGATATTATTATACGATCTGATACATCGAGACTGA", "aa": "MFLYLILRTHSYSKQENKKDLEELIESFSPSEIVNNSKIVNNEEASILNLTKKFLKIPSNISCDDKVFIETLWILLYDLIHRD", "aa_hexdigest": "9180f33b6fadb241360b98b8ade348c2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9803.179599999992, "isoelectric_point": 5.31174030303955 }, "id": "NKOCPLAKPK_1108", "locus": "GCA003260355_01072" }, { "type": "cds", "sequence": "contig_1", "start": 582396, "stop": 582524, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTTCAAAGGAGGGAGAAATATTTTCTGATAATTTGAATATAGTTGTCAAAAAAATTGCTAAAAACTCAGTGATCGTCTTTACTTCTATAGTTAGAAATATTCTGAATAATATCGTACATTATTGA", "aa": "MFSKEGEIFSDNLNIVVKKIAKNSVIVFTSIVRNILNNIVHY", "aa_hexdigest": "cbd262e5249244bb623ac93a4ac31d07", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4808.5978, "isoelectric_point": 9.398990821838382 }, "id": "NKOCPLAKPK_1109", "locus": "GCA003260355_01073" }, { "type": "cds", "sequence": "contig_1", "start": 583803, "stop": 583940, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAAGAATGAATAAAATTGATGATTGCTTAAATCTCTCTCCAATAATAAAGGACTATATAGAGTATCAATTATTAGAACAAGATGAAGAAAAAGGAAAATATACAATAAAATCGGGAATGAATTTTATTACTTGA", "aa": "MQRMNKIDDCLNLSPIIKDYIEYQLLEQDEEKGKYTIKSGMNFIT", "aa_hexdigest": "96df7775c1630ab11a8baa253192c5de", "start_type": "ATG", "rbs_motif": "TTATT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5371.143999999999, "isoelectric_point": 4.7658586502075195 }, "id": "NKOCPLAKPK_1110", "locus": "GCA003260355_01074" }, { "type": "cds", "sequence": "contig_1", "start": 583928, "stop": 584188, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACGGATCCTTTTCCGATTTTGATTAATTATATTTATCTTTTCAATTTTTGTCTCAATATATACCACCTATCAATATCAGATATTTTTATCATATTCTGTTTCATGAACTTGGGCAACAATTTCCGAAACAGTATTCTTGAAAAAATTTTGCTTAAATTTTCATTGGATATACTTAAATTTTCATTGGTGTTTTTTTTTAAGATTTGTTATGTTACGATTTATCTGTTTTTTATTTTCTTTACCGGATGCAAAGAATGA", "aa": "MTDPFPILINYIYLFNFCLNIYHLSISDIFIIFCFMNLGNNFRNSILEKILLKFSLDILKFSLVFFFKICYVTIYLFFIFFTGCKE", "aa_hexdigest": "16463172f3faeb8de17373ed8c52c105", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10387.411399999988, "isoelectric_point": 7.640376853942871 }, "id": "NKOCPLAKPK_1111", "locus": "GCA003260355_01075" }, { "type": "cds", "sequence": "contig_1", "start": 584303, "stop": 584572, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCTATCTTAAATGATAATAGAAATAGTAATGTGGTAGAGAATAATAATGCGATAAATCCATCACGATTATTAGCGATGAAAGTTTTAAACTTTTCCATGATTAATAAAAAAAACTCAATAAAGAATTATTTTATTCATATTTTTTTAACCAGTGTTTCAAGGTATGTTATTATTTATAAACTTTATGTATCATTCCACGATACTTATTTCTTCTATGGATTTACCAAGTTTTGGGCCAAGAGATTATTTTTTTTCGCAAACGAGTAA", "aa": "MSILNDNRNSNVVENNNAINPSRLLAMKVLNFSMINKKNSIKNYFIHIFLTSVSRYVIIYKLYVSFHDTYFFYGFTKFWAKRLFFFANE", "aa_hexdigest": "5afa3be4be272e1cf3df7edd981641ef", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10694.28649999999, "isoelectric_point": 9.928727531433104 }, "id": "NKOCPLAKPK_1112", "locus": "GCA003260355_01076" }, { "type": "cds", "sequence": "contig_1", "start": 584633, "stop": 585175, "strand": "-", "frame": 3, "gene": null, "product": "Methyltransferase type 11 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4IJK9", "UniRef:UniRef90_A0A328EGX3" ], "nt": "ATGACCTATGATAGCAAACATGTAGAACTTCTTATTCCAGAGGAGGGCTATAACCGTACCAAAGATGAATATAAAAAATATCGACCTCATCTGAATTCATTTTATAGTTTGGATTTTAGGAGATTTGTCCCTAGAGAGAGAAACGGGTTTCGTATCTTGGATCTCGGAGCAGGGGATGGGAGAATGTATGATCAGCTCAAAGTCATCAATCCTGATGAATACATTGCCTGCGACTGTGCAAAAGAGCTTCTCTCCAAGCATCCTGGAAGAATCAAAAAAGTCGTTTGTGATTTAGAGAAAGACCGACCCTTTGAAGACGAATCGTTCAATCTCCTTTCAGCTTTTTTTCTCTTGGAGCATATCGAAAATACTGAGCATTTTTTCGCTGAAGCTTATCGTATTTTGCAGCCAGAGGGGCAACTTTTGATCGGTCATTTTCTCCAGAGAAGGCTTTTTACTCGGACAATTAACGGAGAAAAATTTAAAATTGAGCAATTCCCTCATACGATCCAAGAATTAGCAAAAACGGCAGAAGAAAGCTGA", "aa": "MTYDSKHVELLIPEEGYNRTKDEYKKYRPHLNSFYSLDFRRFVPRERNGFRILDLGAGDGRMYDQLKVINPDEYIACDCAKELLSKHPGRIKKVVCDLEKDRPFEDESFNLLSAFFLLEHIENTEHFFAEAYRILQPEGQLLIGHFLQRRLFTRTINGEKFKIEQFPHTIQELAKTAEES", "aa_hexdigest": "4c9d2abdd25e780d5fc4953adc3def67", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A328EGX3", "query_cov": 1.0, "subject_cov": 0.8653846153846154, "identity": 1.0, "score": 370.0, "evalue": 2.29e-129, "valid": true, "product": "Methyltransferase type 11 domain-containing protein", "uniref50_id": "UniRef50_A0A2W4IJK9" }, "pscc": { "uniref50_id": "UniRef50_A0A2W4IJK9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4IJK9" ], "product": "Methyltransferase type 11 domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_1113", "locus": "GCA003260355_01077" }, { "type": "cds", "sequence": "contig_1", "start": 585203, "stop": 585367, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCCTGAGCTTCAGGACTATCCTTTAGAAAAGATGAAATTAAGTATAGACTTCGCAAAATGGGAGGAGATTCTTGTCCAGAAACGAGGTTTCAAGAATCTACAGAAGATTTTACAAGAAGAAAAGAAAAAAACTGAGCAACCTCAGCAGTTAGGGCTTTTTTAG", "aa": "MPELQDYPLEKMKLSIDFAKWEEILVQKRGFKNLQKILQEEKKKTEQPQQLGLF", "aa_hexdigest": "6c993fdaf6104b3bbb0e2664411fc95b", "start_type": "GTG", "rbs_motif": "TTATT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6534.599899999999, "isoelectric_point": 7.98577938079834 }, "id": "NKOCPLAKPK_1114", "locus": "GCA003260355_01078" }, { "type": "cds", "sequence": "contig_1", "start": 585413, "stop": 586093, "strand": "-", "frame": 3, "gene": null, "product": "5'-3' exonuclease domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A288DD38", "UniRef:UniRef90_A0A328EKX1" ], "nt": "ATGAAAACCCTTTCACTCGTTGATGGATCAGGCTTTCTCTTTAGAGCTTGGTATGCTTTCCCTCCGATGATCAATGCCCAAGGGCAAAATCAGAATGTAATCTATGGTTTTATCCGTATGATGCTGAAACTCCTCTTTGAACAGCCGGAATATTTTGTGATTTGCTGGGATTCTCCCATCAAAACCAAGAGAAAAGAGAAGTATGAAGCCTACAAAGCAAATAGAACGAAGCTGGAAGATGAGTTCAAAGCTCAGATCCCTTTAGTTATGGAGTTGGTTTCCGAGCTCGGGATTCCTACGATTACGGCTCCTGGCTATGAAGCAGATGATAGTATTGGGACTTTAGCTTCTTTTCATCAGAAGAATCCTGATCTTTTGGTTCAGGTTTTTAGTTCTGATAAGGATTTAAAACAGCTCCTCGCTCCAAATCTCGTCGTAACCGATCCTCTCAAACATATCACCACGAGAATCCCCGATTTTGAGAGAGAGTTTGGCTTTGCTCCCGTGTCAATTGTCGATTATTTGGCGTTGATTGGAGATGCCGCAGACAATGTCAAAGGAGTTGCGGGGATTGGACCCAAAAAAGCTTCCGATTTGATCAAAAAATACGGAACAATAGAGCAAATCTATACTCATCTTGATGAAATCCAAGGTGAAATAAAAGAAAAACTGATCAACTGA", "aa": "MKTLSLVDGSGFLFRAWYAFPPMINAQGQNQNVIYGFIRMMLKLLFEQPEYFVICWDSPIKTKRKEKYEAYKANRTKLEDEFKAQIPLVMELVSELGIPTITAPGYEADDSIGTLASFHQKNPDLLVQVFSSDKDLKQLLAPNLVVTDPLKHITTRIPDFEREFGFAPVSIVDYLALIGDAADNVKGVAGIGPKKASDLIKKYGTIEQIYTHLDEIQGEIKEKLIN", "aa_hexdigest": "2158e151a581a20aa1b540db580daa96", "start_type": "ATG", "rbs_motif": "TTATT", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 585203, "stop": 586093, "observations": { "mutation": [ 585415 ], "stop": [ 585415 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EKX1", "query_cov": 1.0, "subject_cov": 0.7635135135135135, "identity": 0.996, "score": 447.0, "evalue": 8.1e-158, "gene_start": 1, "gene_end": 226, "reference_start": 1, "reference_end": 226, "reference_sequence": "MKTLSLVDGSGFLFRAWYAFPPMINAQGQNQNVIYGSIRMMLKLLFEQPEYFVICWDSPIKTKRKEKYEAYKANRTKLEDEFKAQIPLVMELVSELGIPTITAPGYEADDSIGTLASFHQKNPDLLVQVFSSDKDLKQLLAPNLVVTDPLKHITTRIPDFEREFGFAPVSIVDYLALIGDAADNVKGVAGIGPKKASDLIKKYGTIEQIYTHLDEIQGEIKEKLINGKEDAFFSKELILLMQVPELQAYPLEKMKLTIDFAKWEEILVQKRGFKNLQKILQEEKKKTEQPQQLGLF" }, "paralog": false, "identity": 0.9864864864864865, "score": 579.0, "evalue": 4.1e-212, "description": "Internal stop codon at 585415. Nonsense mutation around 585415.", "psc": { "uniref90_id": "UniRef90_A0A328EKX1", "product": "5'-3' exonuclease domain-containing protein", "uniref50_id": "UniRef50_A0A288DD38", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EKX1", "UniRef:UniRef50_A0A288DD38" ] }, "pscc": { "uniref50_id": "UniRef50_A0A288DD38", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A288DD38" ], "product": "5'-3' exonuclease domain-containing protein" } }, "id": "NKOCPLAKPK_1115", "locus": "GCA003260355_01079" }, { "type": "cds", "sequence": "contig_1", "start": 586166, "stop": 586378, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGGATTGTTTGCTACGTTTGGAACTATCGCTCAGGGAAGGAAAACTACTTTCCAGTTTTCTACCAATGACGCCGAATTGCTCGGACTTTTCGTGCAACGGTGGAATGAGAAAAAGTCTGATTCACCTTTTTCATTAGAAAATTTTGAGAAGAAGCAGCTCGAAATCAAAGATCAGCTTTTGGATTTTATTGCCTCTGAAGAATGCTCTTGA", "aa": "MGLFATFGTIAQGRKTTFQFSTNDAELLGLFVQRWNEKKSDSPFSLENFEKKQLEIKDQLLDFIASEECS", "aa_hexdigest": "3c15638c96829461a0559738070e93d0", "start_type": "TTG", "rbs_motif": "TTTTT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8025.940999999998, "isoelectric_point": 4.761254692077636 }, "id": "NKOCPLAKPK_1116", "locus": "GCA003260355_01080" }, { "type": "cds", "sequence": "contig_1", "start": 586712, "stop": 586978, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCACCTTACTTTTGACCAGCATCATTTACTCTGTGTTGAGAATCCGAATATCCCTCAGCTCAAGGAGTATCGTTTTTCGCTCTCCGGCTACCAAATTTCAAGCTATGATAAGGGGATTCTTGTCTATCACAAGAGGCAACGAAAGCTAATGAACCTCAAGAATTTAGGAGAAGGGATGCAGGTTTGCTATCTCCAAGATCAGCCGCTTCCAGAATACAAGCTTAATATCTCCATGCTGGAGAGGACGCTTGCAATGTTTTCTTGA", "aa": "MHLTFDQHHLLCVENPNIPQLKEYRFSLSGYQISSYDKGILVYHKRQRKLMNLKNLGEGMQVCYLQDQPLPEYKLNISMLERTLAMFS", "aa_hexdigest": "9ce0777f4e39f924e9946e13f9bd7a38", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10448.070399999999, "isoelectric_point": 8.601968193054201 }, "id": "NKOCPLAKPK_1117", "locus": "GCA003260355_01081" }, { "type": "cds", "sequence": "contig_1", "start": 587007, "stop": 587546, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATTGCCTTGATCGGAGTAGGAACCATCAGTATGTGGAATCCCGACAACTATGCCTTGACCAGACTTAAAACCTTTTTCACCACCGATGAGGAAGCCAAAAAGGAAATCTCCAAAAAAGATGGCCGACAAAACCAGCAGGGAATCATCGCCATTGGCGGAGGAGGCTTTCGAGGGCAGGGCTACGGGAAAGGGCTCCAAAAAATGGGGCAATTACCAGAGGCCTATTCTGATATGATCTTTGCTGCGTTCTCAGAAGAGATCGGATTTATCGGGAACATGATCCTCTTTGCTCTCTATATTGGGCTCTTCTTCACCGTGCTAAAGCAACTCGATCAAGTAAGAGATCCCCAGCTCAAAACCCTTGCCGTCGGAATTATTTCGCTGATCATTGTGCAGGTTTTCGTCCATGTTGGAGTAAATCTCGAGATCCTCCCAAATACAGGACTTACTTTACCATTCATTAGTCATGGTGGGACTGCACTGATGATCAATCTGATAGAACTTACCTTACTCTACAAGATCCTCAGAGGGAAATAA", "aa": "MIALIGVGTISMWNPDNYALTRLKTFFTTDEEAKKEISKKDGRQNQQGIIAIGGGGFRGQGYGKGLQKMGQLPEAYSDMIFAAFSEEIGFIGNMILFALYIGLFFTVLKQLDQVRDPQLKTLAVGIISLIIVQVFVHVGVNLEILPNTGLTLPFISHGGTALMINLIELTLLYKILRGK", "aa_hexdigest": "91d95401cc3c2ba676eabaea5e4c3ae3", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19651.93170000002, "isoelectric_point": 8.830573081970215 }, "id": "NKOCPLAKPK_1118", "locus": "GCA003260355_01082" }, { "type": "cds", "sequence": "contig_1", "start": 587568, "stop": 588104, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCTACGCTTGCGACGATTGAGGCGGAGACCGGCGAGGAGGTAAAACTCGTAGCAAACGAAGAAACCAGGATCCAAGCAAAGAATAACTACTTCTATTTTTCCAGTCAGATCAAGAATCTTGCGGTAGCATTGATTGTCGTTTTCTTGACTCTGATTTTCCCGCTCCCTTTACTCAAGGACAAAAAGCTAGCAATTCTGATTGCAGTGGCGGTTTTTCTCTTCCAGCTCTGTGTGTTTATCCCCGGGATCAAGGCAACAAACGGAACTGCGAGAGGCTGGGTAGACTTCCCTGGACTCCCGAATATGCAACCTGCGGAGTTTTTCAAGCTGGGATACGTATTTTTTATGTCCTATTGGATTTCCAAGAGGAGAGAGGTCATCAAATCAGCACCGTTCCTGACCCAGTTTGCTCTGATCAATGCGATTATTCTTTTGGTGATCCTTGCGATTCCTGACATGGGGACGCTCTTCATTCTCGCTTTGACCGGGACGATCATGGCATGGTATAATGGCTTAAAAATCGCAAGAGTCTGA", "aa": "MPTLATIEAETGEEVKLVANEETRIQAKNNYFYFSSQIKNLAVALIVVFLTLIFPLPLLKDKKLAILIAVAVFLFQLCVFIPGIKATNGTARGWVDFPGLPNMQPAEFFKLGYVFFMSYWISKRREVIKSAPFLTQFALINAIILLVILAIPDMGTLFILALTGTIMAWYNGLKIARV", "aa_hexdigest": "5b142c22b7bc81ae14538ca3ce758da0", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19921.693500000005, "isoelectric_point": 9.409950447082519 }, "id": "NKOCPLAKPK_1119", "locus": "GCA003260355_01083" }, { "type": "cds", "sequence": "contig_1", "start": 588144, "stop": 588284, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGACGATCATCCAAAATATCGAAACCGTCAAAGCGTTCAACACCTTTCTCGATGAAACTATCCTCAAAACGACACAAAAAATCATACAAAAGTCTGAACCTTTGATTGTGGTTGACACGACGGGGTCCAACCTTTCCTGA", "aa": "MTIIQNIETVKAFNTFLDETILKTTQKIIQKSEPLIVVDTTGSNLS", "aa_hexdigest": "7ff92b9c9655ab97b34eb5c80bab353a", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5137.8944, "isoelectric_point": 4.93575038909912 }, "id": "NKOCPLAKPK_1120", "locus": "GCA003260355_01084" }, { "type": "cds", "sequence": "contig_1", "start": 588575, "stop": 589270, "strand": "+", "frame": 2, "gene": null, "product": "Thioredoxin domain-containing protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DC00CB0", "UniRef:UniRef100_A0A328EH15", "UniRef:UniRef50_A0A328EH15", "UniRef:UniRef90_A0A328EH15" ], "nt": "ATGAAATTAGGTGTCAGTGTTTTTGCTCTTTTGGGAGTTGCTTTGCTTGCGGGGTGTCAGCCTACACAACCAAGTGAACCCACTCTACCACATCAGCCTTCGGTGGTTGTCAAAAACGATACTCAGGCGTTTAAGACGGAATACGAATCACTCAATGCGGAAAAGATGCCGATCACCGTGCCTGAAGATTTGAGCATTTCTGTGCTTGATTTCTCTGGGACGAAAGAATTTTTGGAGAAAGGGACAGGAATCCTTTTCTTTGGATTCCCAACTTGCCCATGGTGTAGAGCCTTACTTCCAAACCTCTTTGATGCAATGAAAAAGAGTCAGATTTCTGATCTTTATTACTACAATCCTAAAGAAATCCGTGACCAAAAATCACTCGGAGCAGACGGAAAAATCGTTGTTGAAAAGGAGACCAGCCCAGACTATCAGTATTTGCTCGAGAAACTTGATAGCGTGCTTCCAGCCTATGAGGGATTGAAGGATCCGAGCATCAAAAGGCTGTATGTGCCGTTTGTCGTGGTGCTGAAGGAGGGGAAAATCGTGGGATCTCACCTCAACACGCTCGATGAGCAAGAGGATCCTAGCGTTCCGCTTACTGATGAGCAGAAGGCGAAGCTTGTGGAATTGCTCGAGCAGGCGATGGCGCCCCTTGCCTCACAGACGTGTACTCCTGGAGAGCTTGCTTGCTAG", "aa": "MKLGVSVFALLGVALLAGCQPTQPSEPTLPHQPSVVVKNDTQAFKTEYESLNAEKMPITVPEDLSISVLDFSGTKEFLEKGTGILFFGFPTCPWCRALLPNLFDAMKKSQISDLYYYNPKEIRDQKSLGADGKIVVEKETSPDYQYLLEKLDSVLPAYEGLKDPSIKRLYVPFVVVLKEGKIVGSHLNTLDEQEDPSVPLTDEQKAKLVELLEQAMAPLASQTCTPGELAC", "aa_hexdigest": "b95f8554b6f5c4e629b5c4158ee6b422", "start_type": "ATG", "rbs_motif": "TTTTT", "ups": { "uniparc_id": "UPI000DC00CB0", "uniref100_id": "UniRef100_A0A328EH15", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DC00CB0", "UniRef:UniRef100_A0A328EH15" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EH15", "uniref90_id": "UniRef90_A0A328EH15" }, "psc": { "uniref90_id": "UniRef90_A0A328EH15", "product": "Thioredoxin domain-containing protein", "uniref50_id": "UniRef50_A0A328EH15" }, "pscc": { "uniref50_id": "UniRef50_A0A328EH15", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EH15" ], "product": "Thioredoxin domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_1121", "locus": "GCA003260355_01085" }, { "type": "cds", "sequence": "contig_1", "start": 589478, "stop": 589621, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGCCCAGATTTGGGTGTCGGACAGGTCGATGATGCAAGAATGACCCCCAAATTTGACTTCCGAGCTGCCGGAAAGGCGAAAATGCCCCCCAAATTCGATGCTCGGGGTGGTCGGAAGGGTAAAACGGACCCCCAAATTTGA", "aa": "MSPDLGVGQVDDARMTPKFDFRAAGKAKMPPKFDARGGRKGKTDPQI", "aa_hexdigest": "e5d754193fefde7ee90f6a39e30566af", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5090.819700000002, "isoelectric_point": 9.989134407043458 }, "id": "NKOCPLAKPK_1122", "locus": "GCA003260355_01086" }, { "type": "cds", "sequence": "contig_1", "start": 589664, "stop": 589834, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTCGGGGTGATCGGAAGGGTAAAATGGACCCCCAAATTCGATGCTCGGGGTGGTCGGAAGGGTAAAATGACCCCCAAATTTGATGCTCGGGGTGGTCGGAAGGGTAAAATGACCCCCAAATTTGATGCCCGGGGTGGTCGGAAGGGTAAAATGATCCCCCAAATTTGA", "aa": "MLGVIGRVKWTPKFDARGGRKGKMTPKFDARGGRKGKMTPKFDARGGRKGKMIPQI", "aa_hexdigest": "a31eb77fb577688c0f0ea2467524549f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6202.407800000003, "isoelectric_point": 11.905972862243651 }, "id": "NKOCPLAKPK_1123", "locus": "GCA003260355_01087" }, { "type": "cds", "sequence": "contig_1", "start": 589937, "stop": 590572, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAGAAATATCATCGGACAGCCAAAATCCGCCTTCAAAATGTATGATGCAATGCAGCAAAAGAAAATCATTCTCATGAACCTCGCCAAAGGTATCGCTGGAGAGGAAACTTCAAAACTGATTGGAAAGATTATCGCTATGCAGGTAAAACTTTCAGCATTGAAGAGGGCTAAAATCGATGAGAAGGACAGAGTTCCTCACTATCTCTATGTGGATGAGTTCCAAAACTATGTCTCTCAGTCTTTTGAATCTATTCTTTCAGAGGCGAGAAAATATAAAGTGGGACTCGTGGTTGCCCATCAGTATACCGACCAGCTGAAGCAGGAAGGACTCGGCGGGAATATGGACCTCTCCAAGACCATTTTTGGTAATATCGGAAATATGTTCGTCTTCAAGGTCGGAGCTCCCGACGCAGAATTCCTTACCAAAGAGTTTGAACCTGAATTCAACCAAACCGATTTGACCTCTACAGCAGCCTTTATGGGCGCATGTAAGGTTTCTGTCAATAACCAGCAGACAAGACCCTTCAGCTTTGAGGCGAAAGTGCTGTATCCCGGTTTCCCAGACGGCCTTCCTTACGCCAATTCTCCCGAGAAAGTCCAAATCATCAAACAAATTTCCTCCCTCAAACGATGA", "aa": "MRNIIGQPKSAFKMYDAMQQKKIILMNLAKGIAGEETSKLIGKIIAMQVKLSALKRAKIDEKDRVPHYLYVDEFQNYVSQSFESILSEARKYKVGLVVAHQYTDQLKQEGLGGNMDLSKTIFGNIGNMFVFKVGAPDAEFLTKEFEPEFNQTDLTSTAAFMGACKVSVNNQQTRPFSFEAKVLYPGFPDGLPYANSPEKVQIIKQISSLKR", "aa_hexdigest": "0ea043a54f75cbe8ca49aaf2b4839ddc", "start_type": "GTG", "rbs_motif": "TTTA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 23704.161399999997, "isoelectric_point": 9.231373023986816 }, "id": "NKOCPLAKPK_1124", "locus": "GCA003260355_01088" }, { "type": "cds", "sequence": "contig_1", "start": 590594, "stop": 591085, "strand": "-", "frame": 3, "gene": "herA", "product": "Helicase HerA central domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A351Y4F8" ], "nt": "ATGCTTGCTCAGGCAGTCGATGATATAAAAAACGGCAGAGGCTTTACTTTTATTGATCCCCATGGAGAAGCTTGTGAAGCACTTTTGAAATGGTTCCCTAAGGAGAGGATCGATGACCTCATTTATTTCGATTTGGGGAATACCGCTTATCCGATCGGCCTCAATCCGCTCGAAGTTAACCCTGAGGACAGCGATGAAAAGGACGTCGTGACTAATGACCTGGTAGAAATGTTTATCCAAATGTATGGACCAGAAATTTTCGGACCAAGAATCCAGGATTACTTCCGTAATGGATGCCTTTTACTCATGGATCAGCCCGAAGGCGGCACCATTGTTGATATTATGAGACTCTTTACGGACGATGCTTTTGCCGAAGCAAAAATCAGAAACCTCAAAGACCCGGTAGTCGCAGCTTGGTGGAATAAAACCTACAAAAGGATGGGGGACCGTGAAAAAGCAGAAATTATTCCTTTCATCCAGGCAAAATTCTGA", "aa": "MLAQAVDDIKNGRGFTFIDPHGEACEALLKWFPKERIDDLIYFDLGNTAYPIGLNPLEVNPEDSDEKDVVTNDLVEMFIQMYGPEIFGPRIQDYFRNGCLLLMDQPEGGTIVDIMRLFTDDAFAEAKIRNLKDPVVAAWWNKTYKRMGDREKAEIIPFIQAKF", "aa_hexdigest": "933948bb41fee8d130b5364dc2882019", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A351Y4F8", "query_cov": 1.0, "subject_cov": 0.806930693069307, "identity": 0.7240000000000001, "score": 254.0, "evalue": 3.37e-84, "valid": false, "product": "Helicase HerA central domain-containing protein", "uniref50_id": "UniRef50_A0A351Y4F8" }, "pscc": { "uniref50_id": "UniRef50_A0A351Y4F8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A351Y4F8" ], "product": "Helicase HerA central domain-containing protein" }, "genes": [ "herA" ], "id": "NKOCPLAKPK_1125", "locus": "GCA003260355_01089" }, { "type": "cds", "sequence": "contig_1", "start": 591176, "stop": 591745, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3D0UT19" ], "nt": "ATGAGAAAGAGAGCCGACGAGATCAAAGAAGGTAAAAAACCGCTGGGCATTCTCGGCATCCTCAGCAAAATTCGAAATGCGGGCGGAGAAAAGGAGCAGAAAGAATCCAAAAAGGAACAAGAACAAAAGAAACACAACTTCTCCCAGCAGCAGCTGAGCGATTTTGACAAGAAGATGGATGACGAACTCTTCCAGGTGAAGATGAGAACGATCGCTACTTCACCAGACAAAGCCCGTCCCAAGAAAATCTTGGACGACATCGCTCGTCTCTTCAATCAGTATAACTATATCGGACTCAATACCATCAAATTTACCAAGGCACAGGACCTCAGAAAGTTTGCTAAACACTATGTGATGAGACTTTTTTATAGTGATAATACTTTGCTGGACGACATCCGAAAGTTTAATAAGACGACGATCCTCAATATCAAGGAACTCTCTTCTATTATTCACTTTCCTCACGGCAGATTCAACCAAAACCCAAGAATCGCTTGGCAAAAATTCAAGATCGTTCCCGCCCCAGACGACCTTCCTAGCGAAGGAATCGTTGTCGGCTACAATAACTTCTGA", "aa": "MRKRADEIKEGKKPLGILGILSKIRNAGGEKEQKESKKEQEQKKHNFSQQQLSDFDKKMDDELFQVKMRTIATSPDKARPKKILDDIARLFNQYNYIGLNTIKFTKAQDLRKFAKHYVMRLFYSDNTLLDDIRKFNKTTILNIKELSSIIHFPHGRFNQNPRIAWQKFKIVPAPDDLPSEGIVVGYNNF", "aa_hexdigest": "99463530ca70a8b2f9a465507079139f", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3D0UT19", "query_cov": 0.8201058201058201, "subject_cov": 0.8469945355191257, "identity": 0.523, "score": 177.0, "evalue": 2.45e-53, "valid": false, "uniref50_id": "UniRef50_A0A3D0UT19" }, "pscc": { "uniref50_id": "UniRef50_A0A3D0UT19", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3D0UT19" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 22129.262100000004, "isoelectric_point": 9.796502876281735 }, "id": "NKOCPLAKPK_1126", "locus": "GCA003260355_01090" }, { "type": "cds", "sequence": "contig_1", "start": 591791, "stop": 592390, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAACAAAATCTAATCTATCTTTTGATTAGCGTTATCGCTGCTGGAGTGCTCTGGATTGCGGGGGTTATCGTTGCTAGAGCTGTTTTTTTGTCATTTCAGAGAAAACACAGAATCGACCAATCCAGAAAAATCAGATTCCTTCAGGTGCAGATCCCAAAGAATGCAGTAGCGAGGAGTTCTGACATTGATGCGAAGGATCATATCCAGTCAATGAAGCACAATATCGAACTAATGAATCAGGTGTACAAAAATTTCTATGCGATTTTCGATCAAGGGCGAGAAAATAAACGATTCGGAAATAATTTCATCAGTATGGAAATCATCGTTGAAAAAGAGGTGATCAAATTCTACCTCGGTGTGCCACGGGAGCACCTCATGACCGTGGAAAAAATGATCGCCTCCTTTTATGTCGGTGCAATCGTCAGAGAAGTCAAGCAGCCCAAACTCCTGGAGGCAGGAAAATTCTTCGCAGGAGGGGAGTTTACCCTGACCAAAGACAGCGTTCATCCGCTCAAAACCTATGAATCCTTCGAGGCAGACCCAATGGATAGTATTCTGTCAGCTTTTTCTAATATTTCTAGAGATGAAAAAGTCTGA", "aa": "MEQNLIYLLISVIAAGVLWIAGVIVARAVFLSFQRKHRIDQSRKIRFLQVQIPKNAVARSSDIDAKDHIQSMKHNIELMNQVYKNFYAIFDQGRENKRFGNNFISMEIIVEKEVIKFYLGVPREHLMTVEKMIASFYVGAIVREVKQPKLLEAGKFFAGGEFTLTKDSVHPLKTYESFEADPMDSILSAFSNISRDEKV", "aa_hexdigest": "f9f315d77af694b46acbb51e44c417b8", "start_type": "ATG", "rbs_motif": "TTTA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 22861.323800000006, "isoelectric_point": 9.197075843811035 }, "id": "NKOCPLAKPK_1127", "locus": "GCA003260355_01091" }, { "type": "cds", "sequence": "contig_1", "start": 592455, "stop": 592628, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTGTTTGTGTCTTTGTGAGCTTAGTCCTCGTCCGTGGATTTATTTTTGCTTGCGGTGAGTCTTCGAGAGTCTTTTTTGTCCAAATAGGTTTGAATTTGCTCGGGGGTGAGCTTCTGCTCTTTGACGAGCGTGTTGATTTTGTGGTAAGGGAGATCCATTACCTCGTCTAA", "aa": "MFVCVFVSLVLVRGFIFACGESSRVFFVQIGLNLLGGELLLFDERVDFVVREIHYLV", "aa_hexdigest": "217aef388493725c4033cd61a6e35caf", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6512.703899999997, "isoelectric_point": 5.00463924407959 }, "id": "NKOCPLAKPK_1128", "locus": "GCA003260355_01092" }, { "type": "cds", "sequence": "contig_1", "start": 592869, "stop": 593264, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF12705.13" ], "nt": "TTGGATAAAGAGGGCGATGCCTTCGTTATCAACGACTACAAAACCAATAAACAGCTCCCTCCCGAGCAAAAAGAAGAATATAGAGAACAACTCACGCTCTATGCACTTGGTGTCCAGCAGAAGTATGGGAAATATTTTCAAAAGATTAAAGCCAGACTTCATTACTTGCATTTTGATTTGAGGGATGAGCGGGAGATCACAGATGCTTTACTTGCCCCAATCCTCGAGAAATACCGCACTGCCATCAATGCTATAGAAAAAGCCAGATTCAACTACAATATGGGACGGGAAAATGCATTTCCGACTCAGCAAAACCCTTACTGTAAATACTGCGAATATCAACAGCTTTGCCCGCTTTGGAAGCACCTCGGCTGCGGAGATGAACTCGTGGAGTGA", "aa": "MDKEGDAFVINDYKTNKQLPPEQKEEYREQLTLYALGVQQKYGKYFQKIKARLHYLHFDLRDEREITDALLAPILEKYRTAINAIEKARFNYNMGRENAFPTQQNPYCKYCEYQQLCPLWKHLGCGDELVE", "aa_hexdigest": "f958089c97025693690c48e7c6f9d472", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF12705.13", "name": "PDDEXK_1", "length": 120, "aa_cov": 0.8854961832061069, "hmm_cov": 0.9583333333333334, "evalue": 5.166371153275857e-15, "score": 52.48550033569336, "start": 3, "stop": 118 } ], "seq_stats": { "molecular_weight": 15647.61699999999, "isoelectric_point": 6.31653995513916 }, "id": "NKOCPLAKPK_1129", "locus": "GCA003260355_01093" }, { "type": "cds", "sequence": "contig_1", "start": 593568, "stop": 593702, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF12705.13" ], "nt": "ATGGTCGTCTATTCCTTTTCTCAGATCAATCTCTATCAGCAATGCCCTAGGAAATACCAATATCGCTACCTAGATAAGCTCGAAAGAGAATTTGAAACGACTCCGGACCTTATGCTTGGAACTTGTGTGCATTGA", "aa": "MVVYSFSQINLYQQCPRKYQYRYLDKLEREFETTPDLMLGTCVH", "aa_hexdigest": "92a1658ba5e186b4db00767eec8df98e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF12705.13", "name": "PDDEXK_1", "length": 41, "aa_cov": 0.9090909090909091, "hmm_cov": 0.975609756097561, "evalue": 1.111340519221429e-09, "score": 35.019805908203125, "start": 5, "stop": 44 } ], "seq_stats": { "molecular_weight": 5408.170499999999, "isoelectric_point": 6.51922779083252 }, "id": "NKOCPLAKPK_1130", "locus": "GCA003260355_01094" }, { "type": "cds", "sequence": "contig_1", "start": 593968, "stop": 594162, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCATAAGAAAGCAAAGTTATTAGCTTTAGTTGCTGTAGCTTCAAGTTTGGCAGTAGGAGCTGGCTCACTCGCAGCTCCGCAAACGAATCCTACCACTATCACAACAAAAACGGCAACACAAAAGAAGGATTTTAAAGAGCAGCCAGGCGGTTCTAGAACAGAGCGTCCCATTAAGCTCTCAGAAGAATTCTGA", "aa": "MHKKAKLLALVAVASSLAVGAGSLAAPQTNPTTITTKTATQKKDFKEQPGGSRTERPIKLSEEF", "aa_hexdigest": "3071d84e69cacf5abada8c2ba4591f38", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6751.7202, "isoelectric_point": 9.9959680557251 }, "id": "NKOCPLAKPK_1131", "locus": "GCA003260355_01095" }, { "type": "cds", "sequence": "contig_1", "start": 594697, "stop": 595122, "strand": "+", "frame": 1, "gene": null, "product": "VTC domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EKG0", "UniRef:UniRef90_A0A328EKG0" ], "nt": "ATGAAAAATCAAGCACCAGAAACGACTTTTGAAAGAAGAGAAATCAAATATCTCCTCACCACTGCACAAAAAGAAGCTTTTATGGGAATAATCGAAGGCTATCTTCGTCCTGATGAGAATGGGAACTCAGGAAGTTATCGCTTGATGAGTCTCTATTTTGACTCTCCGAATAAGGATTTTTATCGGGCGAAATGGTTTAATCTCCCTTCTAGAAAGAAAGTCAGAATTAGGAAATATCATAATCCAGAAGAGAAGGGATCTGGAGTGGAGGAACAAGTCTTTATTGAGATCAAAGAACACACTCCTACTATTAATTACAAGAGAAGAATTCTTCTCCCTGTTGGACAGGCGTTTTCGATTCTCAATGGAAACATGATTGAGCAATCGCTTGCTCCTGAAGATCAAGCAATACTTGATGAGATATGA", "aa": "MKNQAPETTFERREIKYLLTTAQKEAFMGIIEGYLRPDENGNSGSYRLMSLYFDSPNKDFYRAKWFNLPSRKKVRIRKYHNPEEKGSGVEEQVFIEIKEHTPTINYKRRILLPVGQAFSILNGNMIEQSLAPEDQAILDEI", "aa_hexdigest": "27f0111e2e5c5f69f8fcb2187c1170b7", "start_type": "ATG", "rbs_motif": "TTCTA", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 594697, "stop": 595425, "observations": { "mutation": [ 595120, 595204 ], "stop": [ 595120, 595204 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EKG0", "query_cov": 1.0, "subject_cov": 0.5, "identity": 0.95, "score": 266.0, "evalue": 3.77e-88, "gene_start": 1, "gene_end": 141, "reference_start": 1, "reference_end": 141, "reference_sequence": "MKPQAPETTFERREIKYLLTTAQKEAFMEIIEGYLRPDENGNSGSYRLMSLYFDSPNKDFYRAKWFNLPSRKKVRIRKYHNPEEKASRVEEQVFIEIKEHTPTINYKRRILLPVGQAFSILNGSVIEQALAPEDQAILDEIGGLVKEFQLQPTAVVSYHRQAYKVKKEDGGLRITFDQNIRFRTDELSLERGDQDKRLIDEGMEIMEIKVKGILPSWTQEALERCGIEQASFSKYSHTIEKSFEGALVPPYGLEDYEDILKEAKEASLPLELWKQRTAFLAD" }, "paralog": false, "identity": 0.9462809917355371, "score": 454.0, "evalue": 1.95e-164, "description": "Internal stop codon at 595120, 595204. Nonsense mutation around 595120, 595204.", "psc": { "uniref90_id": "UniRef90_A0A328EKG0", "product": "VTC domain-containing protein", "uniref50_id": "UniRef50_A0A328EKG0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EKG0", "UniRef:UniRef50_A0A328EKG0" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EKG0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EKG0" ], "product": "VTC domain-containing protein" } }, "id": "NKOCPLAKPK_1132", "locus": "GCA003260355_01096" }, { "type": "cds", "sequence": "contig_1", "start": 595732, "stop": 596310, "strand": "+", "frame": 1, "gene": null, "product": "DUF4956 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EGY1", "UniRef:UniRef90_A0A328EGY1" ], "nt": "GTGCGGGTTGGGCGATTGTATAGGCGAACGCATAAGGGTATAAACTACAATCAAGGTTATGTTCAGACTTTGGTCGTGATGGGTGGAATTATTGCGATGATTATGCTGATTCTTGGCTCTGATGTAGCGAAGGCCTTTACGATGATGGGAGCATTTTCGATTATCAGATTTAGAAATAATATGAAGGAAACAAGAGATATCGGCTTTATCTTCCTAGTGATGGCGATCGGAATGGCAATGGGGACCAAACTTTATCTCCTTGCGGCACTTGGAGCATTGGTGATGTCTGCGATTATCTACGGAATGTATCGCTTTGACTGGTTTGCTCAGAAGAAAAAAGGAAGAATCCTGACGATCCGCATTGCACCAAAGAAGTTTGAAGATGGATTGTTTGATAAAATATTTGAGAAGTATGGAGTCAAAGCTGACTTTCTTGGCTATGAAGGGGTTAAAAAAGAGGATTTAATTCAGATTTCTTTCAGTGTTCAGATTGGACAAAAGACCAATGTTCCTAGTCTCCTAGCTGAATTGCAAGAGAGAAATGGGAATGAGAAGATTAGTCTCAGCTATGCTTCCTAG", "aa": "MRVGRLYRRTHKGINYNQGYVQTLVVMGGIIAMIMLILGSDVAKAFTMMGAFSIIRFRNNMKETRDIGFIFLVMAIGMAMGTKLYLLAALGALVMSAIIYGMYRFDWFAQKKKGRILTIRIAPKKFEDGLFDKIFEKYGVKADFLGYEGVKKEDLIQISFSVQIGQKTNVPSLLAELQERNGNEKISLSYAS", "aa_hexdigest": "54d9239d37c5f8ce1f1d292b3a204d6c", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A328EGY1", "query_cov": 1.0, "subject_cov": 0.8384279475982532, "identity": 0.9790000000000001, "score": 362.0, "evalue": 1.25e-125, "valid": true, "product": "DUF4956 domain-containing protein", "uniref50_id": "UniRef50_A0A328EGY1" }, "pscc": { "uniref50_id": "UniRef50_A0A328EGY1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EGY1" ], "product": "DUF4956 domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_1133", "locus": "GCA003260355_01097" }, { "type": "cds", "sequence": "contig_1", "start": 596448, "stop": 597443, "strand": "-", "frame": 2, "gene": null, "product": "Peptidase C51 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EKX7", "UniRef:UniRef90_A0A328EKX7" ], "nt": "ATGATGTTCGCGGTTGTAGCCCTCTTGAGCTCCTGCGAAAAAGAGGACGCAGCTCCTGTAGTTCCCCAGTATAGAATTGATGATGCGCCTGTTCCGGGACCTGATCCCCATGCTCCGAATTATTTCAGCATACCGAGAGAGGGCGAGTATACGCCGATGGGCTCGCGGAAGCTGACGATAAAAGGAGCCGCTGCAATCTTTCAAGGAGGCGTTATCAAGGCAAAGATCAGTCACAGACAAGGTAATGTCTTTTTCTTGGATATCACCAAGCAAGATGGATCGAAGTTTCGAAATCCTGTAGTTGTCGGCGTCAAATCCGGGTCTGTCTATGGAGAAGTCCATTGGACAAAAGAGGCCTCGGCAGGGGAATCAGTAGTCTTTACTTATTTCCATGCTAGGTTTGCTCGCGGATACGAGCATTTCTATCCGATTGTTATGGACGCCAAAACGGGGGAATGCTATTATGCAGAACCGATTCTGGTCTATACCGATCCGCTCTATGCGAATGTGTGTGGAGAGGGTGAAGTATTCGGGACTGCCAACGGAGTAGAGATCCGTTGTACTGGAGAGAATGCAAGCGCAGATTTCTGTCTCCGTTATATCGAGAAGGTATATCAGGAGAAAATTCAGAACGCTTCAAAGCCAAAAGATTGGTATGCTGATACCAACAACTTCAAGCATTTCAGCCGTTATCCTAACGGTGGTGATGTTGCTCCAAGAGTTGGAGATATTCTGGTTTTCTCTAGTTATAGCCAGATATTCTCTGAGGAAGGGGATGGGCATGTAGCCATCGTTATGGAGGTAACTGATACGTCCATAAGGGTAGCTCAGCAGAATTTCTCGGTCAATGTAGAGAGCTCTTACTGGTACCCAATTGGTGGAGAGTTCAAACGCTCAGGGAATCGCATCATTGATCCTGAACCAGGCAATGTAATGCGCGGCTGCATTGTTACTCCGGAACCAAAGTATTCAATCATCGGTTGGATGCGGTATTAA", "aa": "MMFAVVALLSSCEKEDAAPVVPQYRIDDAPVPGPDPHAPNYFSIPREGEYTPMGSRKLTIKGAAAIFQGGVIKAKISHRQGNVFFLDITKQDGSKFRNPVVVGVKSGSVYGEVHWTKEASAGESVVFTYFHARFARGYEHFYPIVMDAKTGECYYAEPILVYTDPLYANVCGEGEVFGTANGVEIRCTGENASADFCLRYIEKVYQEKIQNASKPKDWYADTNNFKHFSRYPNGGDVAPRVGDILVFSSYSQIFSEEGDGHVAIVMEVTDTSIRVAQQNFSVNVESSYWYPIGGEFKRSGNRIIDPEPGNVMRGCIVTPEPKYSIIGWMRY", "aa_hexdigest": "105f6f940bc5ef041e583cd664d6dd6d", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A328EKX7", "query_cov": 0.9969788519637462, "subject_cov": 0.9593023255813954, "identity": 0.976, "score": 672.0, "evalue": 4.73e-244, "valid": true, "product": "Peptidase C51 domain-containing protein", "uniref50_id": "UniRef50_A0A328EKX7" }, "pscc": { "uniref50_id": "UniRef50_A0A328EKX7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EKX7" ], "product": "Peptidase C51 domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_1134", "locus": "GCA003260355_01098" }, { "type": "cds", "sequence": "contig_1", "start": 598080, "stop": 598451, "strand": "+", "frame": 3, "gene": null, "product": "Viral histone-like protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DC03C74", "UniRef:UniRef100_A0A328EKD5", "UniRef:UniRef50_J9R1I8", "UniRef:UniRef90_A0A328EKD5" ], "nt": "ATGAAATTCAAACTCGTAGAAAAAGCAAATCCTATGGATAGGACCAAGAAAAAGCGGTATGCTAACGCAGTCAATGCTGGGACGATGTCTCAGAAAGAAATGGCAAGGCTCTTGGAAGAGAAGTCCTCGCTCACGATTGGAGATATTTCCAATGTGATTGAAAACTTGATCGTAGAACTTCCAAGGCAACTGATAGAAGGGAAGAGCGTAAAGCTTGGAGATTTCGGTTCGTTTCGCTTGAGCCTTTCAAGTGAAGGGGCTGAGGACAAGAAGAAGTTTAACACGGCAAAGATTGAACCAAAGGTGATCTTTACGCCATCGACTGAATTTAAAGAGAAACTTCAGAAGATTAGCTATACCCAAGCTGAATAA", "aa": "MKFKLVEKANPMDRTKKKRYANAVNAGTMSQKEMARLLEEKSSLTIGDISNVIENLIVELPRQLIEGKSVKLGDFGSFRLSLSSEGAEDKKKFNTAKIEPKVIFTPSTEFKEKLQKISYTQAE", "aa_hexdigest": "31dbd5bf8cba2adc98c0bfdab9eb8a7b", "start_type": "ATG", "rbs_motif": "TTTTTA", "ups": { "uniparc_id": "UPI000DC03C74", "uniref100_id": "UniRef100_A0A328EKD5", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DC03C74", "UniRef:UniRef100_A0A328EKD5" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EKD5", "uniref90_id": "UniRef90_A0A328EKD5" }, "psc": { "uniref90_id": "UniRef90_A0A328EKD5", "product": "Viral histone-like protein", "uniref50_id": "UniRef50_J9R1I8" }, "pscc": { "uniref50_id": "UniRef50_J9R1I8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_J9R1I8" ], "product": "Viral histone-like protein" }, "genes": [], "id": "NKOCPLAKPK_1135", "locus": "GCA003260355_01099" }, { "type": "cds", "sequence": "contig_1", "start": 598636, "stop": 599121, "strand": "-", "frame": 1, "gene": null, "product": "Peptidase family S51 domain protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_U1FKQ5" ], "nt": "ATGATTCAGCTTTTTCTTTGTTCTTCATTTGCAGATGTAGCAAAATTTCTCCCAGACTGCGTAAAAAATCTGCAAGGTATGAAAGTTGCCTTTATCCCGACAGCAGCAGAGGTAGAGAAAGTGAAGTTTTATGTAAAAAAGGGGTGGGAAGCTCTAGAAGATCTAGGAATGCAAATAACAGAGCTTAATATCAACACAACAGAAGCTCCTAAAATAAAGGAAATTATCGAAAACGCTGATTGTATCTATATCTCAGGAGGAAATACCTTTTATCTACTTCAAGAACTCAGAAAGACTGGTGTAGATGAACTAATTAAACAGCATATCCTTACGGGAAAACTTTATATTGGCGAGTCTGCTGGTGCAGTGATTACAGCGCCCAATATTGAGTATGTTTGTTTGATGGATGATCCAAATGAGGGAAAGGAACTGACAAATTTTAAAGCTCTGAATGTGGTGGATTTTTCAACAGTTCCTCACCTCTGA", "aa": "MIQLFLCSSFADVAKFLPDCVKNLQGMKVAFIPTAAEVEKVKFYVKKGWEALEDLGMQITELNINTTEAPKIKEIIENADCIYISGGNTFYLLQELRKTGVDELIKQHILTGKLYIGESAGAVITAPNIEYVCLMDDPNEGKELTNFKALNVVDFSTVPHL", "aa_hexdigest": "0c668c8db2059896fdae36730e61d40a", "start_type": "ATG", "rbs_motif": "TTTTTA", "psc": { "uniref90_id": "UniRef90_UPI0036185CB9", "query_cov": 0.9937888198757764, "subject_cov": 1.0, "identity": 0.563, "score": 172.0, "evalue": 3.95e-52, "valid": false, "product": "Type 1 glutamine amidotransferase-like domain-containing protein", "uniref50_id": "UniRef50_U1FKQ5" }, "pscc": { "uniref50_id": "UniRef50_U1FKQ5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_U1FKQ5" ], "product": "Peptidase family S51 domain protein" }, "genes": [], "id": "NKOCPLAKPK_1136", "locus": "GCA003260355_01100" }, { "type": "cds", "sequence": "contig_1", "start": 599292, "stop": 599495, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTCGGATTGCCAGTCGAGCACTTGAAGGTTTCAGTTTTTAACCTCGTGCATGGTCGCAGAAGCTTCGCTGGTTCAAATATTGGAGGCATTAGAGAGACGCAGGAGATGTTAGATTTCTGCTACCAGCACAATATTTTAGCAGAAGGGGAGGTCTTGCCGATCTCTCAGGCGAATGAGGCTTTTGGGAGGCTAGAGAAGTGA", "aa": "MVGLPVEHLKVSVFNLVHGRRSFAGSNIGGIRETQEMLDFCYQHNILAEGEVLPISQANEAFGRLEK", "aa_hexdigest": "60b0659a98ee7dfe6947f0b52d1ce238", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7454.441099999996, "isoelectric_point": 5.8275541305542 }, "id": "NKOCPLAKPK_1137", "locus": "GCA003260355_01101" }, { "type": "cds", "sequence": "contig_1", "start": 599751, "stop": 600041, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTTGGAGTTGGATGTATTGTAGAATCCTGTCAGCACTGCCAATATTGCGCAGAAGGGGAGGAGCAGTTTTGCGAAAAAGGAGTAACCTTCTCCTTCAATAGTCCAGACAAAGTCTCTGGAGGGATGACCTACGGAGGCTTCTCCAAAACCTATGTCTGCGAAGACAAATACGTACTCCATATGCCACCTTTCCAGAATCTCGCAATGGCAGCTCCACTCCTATGCGCTGGGATTACCGTCTATTCTCCCCTCAAGCACTGGCAAATAGGACCAGGAAAAAAAGTCTGA", "aa": "MVGVGCIVESCQHCQYCAEGEEQFCEKGVTFSFNSPDKVSGGMTYGGFSKTYVCEDKYVLHMPPFQNLAMAAPLLCAGITVYSPLKHWQIGPGKKV", "aa_hexdigest": "f5af765ced447e2138c1f2f3cca2661d", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10475.042699999995, "isoelectric_point": 6.245718574523925 }, "id": "NKOCPLAKPK_1138", "locus": "GCA003260355_01102" }, { "type": "cds", "sequence": "contig_1", "start": 600072, "stop": 600245, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08240.17" ], "nt": "ATGAAACCCTTGGAAATCACTCGTAGAGCACTCAGACCTCAGGATCTCGCATTTGAAGTGCTCTACTGTGGAATCTGTCACTCCGATCTTCACACTATCAAAAATGACCGAGGAACCGCAGTGTTCCCACTTGTCCCAGGACACGAAATTGTTGGGAGAGTAACTGCTATTTGA", "aa": "MKPLEITRRALRPQDLAFEVLYCGICHSDLHTIKNDRGTAVFPLVPGHEIVGRVTAI", "aa_hexdigest": "55edaf4a06a1272024ec55a54a93400f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08240.17", "name": "ADH_N", "length": 45, "aa_cov": 0.7719298245614035, "hmm_cov": 1.0, "evalue": 1.3118543745849352e-09, "score": 34.71565246582031, "start": 13, "stop": 56 } ], "seq_stats": { "molecular_weight": 6369.404, "isoelectric_point": 7.867350959777832 }, "id": "NKOCPLAKPK_1139", "locus": "GCA003260355_01103" }, { "type": "cds", "sequence": "contig_1", "start": 600512, "stop": 600676, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAGAGCTTGTTGTCTGTGCAACGGTACTCGTAGTGGTGCTTGAGCTGGTTGAAGCTATTGTTGAACTTGTAGTTCCACATCCCCCTAGGGTAAGTACTAAACTTATCGCTCACATTAGGAGTATTTTAGATGGTATTGTTTTCATCATATTTCATAGTTAA", "aa": "MLELVVCATVLVVVLELVEAIVELVVPHPPRVSTKLIAHIRSILDGIVFIIFHS", "aa_hexdigest": "c0d7590ee29642ee9be0b7c06f1ef721", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5942.213099999997, "isoelectric_point": 5.773102378845214 }, "id": "NKOCPLAKPK_1140", "locus": "GCA003260355_01104" }, { "type": "cds", "sequence": "contig_1", "start": 601119, "stop": 601634, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF14262.12" ], "nt": "GTGATAGCTGGAGGAGATGGATTAAAATCTGATCATGAAACGCAAGGTAGTATCGCAATGAATGGTGGAGAAGTTAGTATTTCAGCTGGTAGCGATGGAGCCGAAGCCTATAAGACTATTACGATCAACGGTGGAAAACTCAATATCCTCAAATCCTACGAAGGATTAGAATCTGAGATCATTATGATCAATGATGGAGAGATCAGTATTGTCTCTAGTGATGATGGGATCAATATCTCTGATAGTTCTTCAACCACCTCTGAAGGCATGATGCATCGAAATGGTACAGCATCAGGAAGAATCCTGACAATCAACGGCGGAAAGGTTACGATTGATGCTGGAGCTGATGGACTCGATTCCAACGGGGCTATTGAGATGAATGGAGGAACGGTAGTGGTCTTTGGTCCAACAGATAATGGGAATGCGGCACTCGACTACGATGAAACCTTTACGATGAATGGAGGAACCTTACTCGCCTTTGGAAGTAATGGAATAGCGATGAATGTTAGCAACTGA", "aa": "MIAGGDGLKSDHETQGSIAMNGGEVSISAGSDGAEAYKTITINGGKLNILKSYEGLESEIIMINDGEISIVSSDDGINISDSSSTTSEGMMHRNGTASGRILTINGGKVTIDAGADGLDSNGAIEMNGGTVVVFGPTDNGNAALDYDETFTMNGGTLLAFGSNGIAMNVSN", "aa_hexdigest": "aa2a91d9ae0b31c7460d543ebff1f0d9", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF14262.12", "name": "Cthe_2159", "length": 101, "aa_cov": 0.49122807017543857, "hmm_cov": 1.0, "evalue": 2.183606531568673e-18, "score": 62.92231750488281, "start": 3, "stop": 86 } ], "seq_stats": { "molecular_weight": 17246.68739999998, "isoelectric_point": 4.0500284194946286 }, "id": "NKOCPLAKPK_1141", "locus": "GCA003260355_01105" }, { "type": "cds", "sequence": "contig_1", "start": 602084, "stop": 602422, "strand": "-", "frame": 3, "gene": "marR", "product": "MarR family transcriptional regulator", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBFBD7C", "UniRef:UniRef100_A0A328EH68", "UniRef:UniRef50_C7M8G9", "UniRef:UniRef90_A0A328EH68" ], "nt": "ATGAAAATATATCTGAAACGTGTTTATGAGGAGAGAAGTCATGAAGATGGTTTTAGAGTACTTGCAGACCGCCTTTGGCCTCGTGGTATTAAAAAAGAAAATCTTGGTTTAAATCTGTGGGCAAAAGAGATTGCTCCTAGTACTGAGTTGCGCAAACGTTACCATCAAGATGGTAATTTTGAGGCTTTTAGCAAGGATTATATAGAAGAACTCAATCACAATAGTTCCTTCCTTGCTTTTTGTGAGATGCTGAAAGCTCATAAGGTAATTACTTTACTTACCGCGAGTAAAAATATTGAAAAAAGTGCTTTACCTGTATTATTATCTCAGATAGCCTAA", "aa": "MKIYLKRVYEERSHEDGFRVLADRLWPRGIKKENLGLNLWAKEIAPSTELRKRYHQDGNFEAFSKDYIEELNHNSSFLAFCEMLKAHKVITLLTASKNIEKSALPVLLSQIA", "aa_hexdigest": "cbe421e8e8560eb3687c99b6eefbc9ac", "start_type": "ATG", "rbs_motif": "TTATT", "ups": { "uniparc_id": "UPI000DBFBD7C", "uniref100_id": "UniRef100_A0A328EH68", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBFBD7C", "UniRef:UniRef100_A0A328EH68" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EH68", "uniref90_id": "UniRef90_A0A328EH68" }, "psc": { "uniref90_id": "UniRef90_A0A328EH68", "product": "MarR family transcriptional regulator", "uniref50_id": "UniRef50_C7M8G9" }, "pscc": { "uniref50_id": "UniRef50_C7M8G9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C7M8G9" ], "product": "Uroporphyrin-III C-methyltransferase" }, "genes": [ "marR" ], "id": "NKOCPLAKPK_1142", "locus": "GCA003260355_01106" }, { "type": "cds", "sequence": "contig_1", "start": 602583, "stop": 602744, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EK57", "UniRef:UniRef90_A0A328EK57" ], "nt": "ATGAGGACTTGGTATACTGAAGAAGACCTGGAGGACGGATACCTTCCAGGACTTCATTCAACTTATGCTGCTTATGCCGGCCATGAAGATCCGAACTGGAATGATAAGGAGTTTATGGCTGAAGCTAAGGCTCACTATGAGGAGCGAGTAGAACAGGACTGA", "aa": "MRTWYTEEDLEDGYLPGLHSTYAAYAGHEDPNWNDKEFMAEAKAHYEERVEQD", "aa_hexdigest": "8bd6f913e3526350b4718e319997abec", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 602583, "stop": 602906, "observations": { "mutation": [ 602768, 602840 ], "stop": [ 602768, 602840 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EK57", "query_cov": 1.0, "subject_cov": 0.49074074074074076, "identity": 1.0, "score": 116.0, "evalue": 8.63e-33, "gene_start": 1, "gene_end": 53, "reference_start": 55, "reference_end": 107, "reference_sequence": "MIKVNSDLLIWRYRKKYKHKIEGIYCSRNADKEPLIKEGVRIKTLLGARGRDIEMRTWYTEEDLEDGYLPGLHSTYAAYAGHEDPNWNDKEFMAEAKAHYEERVEQDG" }, "paralog": false, "identity": 0.9813084112149533, "score": 221.0, "evalue": 2.48e-76, "description": "Internal stop codon at 602768, 602840. Nonsense mutation around 602768, 602840.", "psc": { "uniref90_id": "UniRef90_A0A328EK57", "uniref50_id": "UniRef50_A0A328EK57", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EK57", "UniRef:UniRef50_A0A328EK57" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EK57", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EK57" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 6310.642699999999, "isoelectric_point": 4.37713680267334 }, "id": "NKOCPLAKPK_1143", "locus": "GCA003260355_01107" }, { "type": "cds", "sequence": "contig_1", "start": 603661, "stop": 603771, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTCCACAGCGATCCAACGACTCATCATACACTCGTTCTTAACCTTAAAAAAATCCAAGGAATGATCAGAAAAATAGATACGCTCATCAAAGAAGGTACTTATTGTTGA", "aa": "MLHSDPTTHHTLVLNLKKIQGMIRKIDTLIKEGTYC", "aa_hexdigest": "2944d08d09efec376dda7745ab2c2884", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4149.9008, "isoelectric_point": 9.03603382110596 }, "id": "NKOCPLAKPK_1144", "locus": "GCA003260355_01108" }, { "type": "crispr", "strand": "?", "repeats": [ { "strand": "?", "start": 604083, "stop": 604118 }, { "strand": "?", "start": 604146, "stop": 604181 }, { "strand": "?", "start": 604208, "stop": 604243 }, { "strand": "?", "start": 604277, "stop": 604312 } ], "spacers": [ { "strand": "?", "start": 604119, "stop": 604145, "sequence": "TTACTTTTTTATTGTAGATATGAAATT" }, { "strand": "?", "start": 604182, "stop": 604207, "sequence": "TCAATGTCTGGAGCCCTCAAGCTCTT" }, { "strand": "?", "start": 604244, "stop": 604276, "sequence": "AAACTCAACAACCATACTATACTCTATAAAAAA" }, { "strand": "?", "start": 604313, "stop": 604323, "sequence": "CTCATAAGAGG" } ], "sequence": "contig_1", "start": 604083, "stop": 604323, "product": "CRISPR array with 4 repeats of length 36, consensus sequence ATCTACAAAAGTAGAAATCTTACATATTTTCTAAAC and spacer length 28", "spacer_length": 28, "repeat_length": 36, "repeat_consensus": "ATCTACAAAAGTAGAAATCTTACATATTTTCTAAAC", "db_xrefs": [ "SO:0001459" ], "nt": "ATCTACAAAAGTAGAAATCTTACATATTTTCTAAACTTACTTTTTTATTGTAGATATGAAATTATCTACAAAAGTAGAAATCTTACATATTTTCTAAACTCAATGTCTGGAGCCCTCAAGCTCTTATCTACAAAAGTAGAAATCTTACATATTTTCTAAACAAACTCAACAACCATACTATACTCTATAAAAAAATCTACAAAAGTAGAAATCTTACATATTTTCTAAACCTCATAAGAGG", "id": "NKOCPLAKPK_46" }, { "type": "crispr", "strand": "?", "repeats": [ { "strand": "?", "start": 604596, "stop": 604631 }, { "strand": "?", "start": 604658, "stop": 604693 }, { "strand": "?", "start": 604722, "stop": 604757 }, { "strand": "?", "start": 604785, "stop": 604820 }, { "strand": "?", "start": 604847, "stop": 604882 } ], "spacers": [ { "strand": "?", "start": 604632, "stop": 604657, "sequence": "GACAAATAGTCAAATATAAAGAATTA" }, { "strand": "?", "start": 604694, "stop": 604721, "sequence": "CTTTTATTCCATTTGCATCCATACAACC" }, { "strand": "?", "start": 604758, "stop": 604784, "sequence": "TTCATAAGAGAAAAGCTACTTCTCATC" }, { "strand": "?", "start": 604821, "stop": 604846, "sequence": "GCACGAAAAGGGAGAATCTCCATCCT" }, { "strand": "?", "start": 604883, "stop": 604893, "sequence": "TCGACTTTTGG" } ], "sequence": "contig_1", "start": 604596, "stop": 604893, "product": "CRISPR array with 5 repeats of length 36, consensus sequence ATCTACAAAAGTAGAAATCTTACATATTTTCTAAAC and spacer length 26", "spacer_length": 26, "repeat_length": 36, "repeat_consensus": "ATCTACAAAAGTAGAAATCTTACATATTTTCTAAAC", "db_xrefs": [ "SO:0001459" ], "nt": "ATCTACAAAAGTAGAAATCTTACATATTTTCTAAACGACAAATAGTCAAATATAAAGAATTAATCTACAAAAGTAGAAATCTTACATATTTTCTAAACCTTTTATTCCATTTGCATCCATACAACCATCTACAAAAGTAGAAATCTTACATATTTTCTAAACTTCATAAGAGAAAAGCTACTTCTCATCATCTACAAAAGTAGAAATCTTACATATTTTCTAAACGCACGAAAAGGGAGAATCTCCATCCTATCTACAAAAGTAGAAATCTTACATATTTTCTAAACTCGACTTTTGG", "id": "NKOCPLAKPK_47" }, { "type": "cds", "sequence": "contig_1", "start": 605319, "stop": 606086, "strand": "-", "frame": 2, "gene": null, "product": "AI-2E family transporter", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4IKW9", "UniRef:UniRef90_A0A328EGZ1" ], "nt": "GTGTTATCAGGGATCATCTTTGTGATTCCGTTCTTGATTTCGCAGATTTCACTCTTGGTACAACGAATTTCTACAACACTTATCGAGCTAAAAAACTTTATCTTGCAAAACCAACGACCAGAAGCGATAAATCAAATTTCACGAATCCCAGATTTCTTAAAAACCTATCTTTTAGAAAATCGAAGCGGATTTTCACGATCAAATACCGATTTTCAGTCAACAATACTCTCAGGTCTCAATACCTTACTTGATACTTCTGCAACTTCACTTAAGTATCTCTCATCAAGATTCTTTTCTTTCGTTAGTGGTTTCTTTGGGGTGGTGACAAATGTGATTATTGTCTTTACGCTCGCTGTCTTCTTCTCTATCGAAAAAAATTACCTTATTTCCCTCTTTGTAAAGGCATCTCATCAAGATCAAAAAGAAAAAACTAGAAAAAAGCTGAATAATATCTATGAAAAGCTTTCCCTCCGACTAAAGGCAAGGATTCTTCTTTCACTCTTCGTGATTTTGGCTATGTATCTCGCTTTTCGAATCTTAAAACTTTTCGGAATTGTAATTCCAAATATCTTTTCTCTCGCTCTGATTTCTGGACTATTGGATATTGTCCCTTATGTTGGACCGCTTTTTGCCTTTATTCCGATCGTGATTCTCGCCTTTATTCATAATGGACTTCGAGGGATGCTTATCGTGGGTTTCGTCTTTCTGCTTGTTCAGTGGCTACAAAATAACGTCATTACTCCATTCCTTATGGAGAAGCAATTGTGA", "aa": "MLSGIIFVIPFLISQISLLVQRISTTLIELKNFILQNQRPEAINQISRIPDFLKTYLLENRSGFSRSNTDFQSTILSGLNTLLDTSATSLKYLSSRFFSFVSGFFGVVTNVIIVFTLAVFFSIEKNYLISLFVKASHQDQKEKTRKKLNNIYEKLSLRLKARILLSLFVILAMYLAFRILKLFGIVIPNIFSLALISGLLDIVPYVGPLFAFIPIVILAFIHNGLRGMLIVGFVFLLVQWLQNNVITPFLMEKQL", "aa_hexdigest": "63054989bb1b434a913703d3cf59a119", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 605196, "stop": 606386, "observations": { "mutation": [ 605321, 606128, 606263 ], "stop": [ 605321, 606128, 606263 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EGZ1", "query_cov": 0.996078431372549, "subject_cov": 0.6240786240786241, "identity": 0.988, "score": 444.0, "evalue": 3.05e-154, "gene_start": 2, "gene_end": 255, "reference_start": 113, "reference_end": 366, "reference_sequence": "MKKTDSAPKKLNLQPEIPTEVSENPLQKKENPRYNNKFSGISTKSMIKFWLIGGLFAFLGYLFMQSLDMIYIIFTAIIIAISMEGMILSFEKRLKTRGGAITIAYIFLLAFVLSGIIFVIPFLISQISLLVQRISTTLIELKNFILQNQRPEAINQISRIPDFLKNYLLENRSGFSRSNSDFQSTILSGLNTLLDTSATSLKYLSSRFFSFVSGFFGVVTNVIIVFTLAVFFSIEKNYLISLFVKASHQDQKEKTRKKLNNIYEKLSLRLKARILLSLFVILAMYLAFRILKLFGIVIPNIFSLALISGLLDIVPYVGPLFAFIPIVILALIHNGLRGMLIVGFVFLLVQWLQNNVITPFLMEKQLGINPILILVSALIGAVVMGFWGVVLSVPLAVIVGIFIDERE" }, "paralog": false, "identity": 0.9747474747474747, "score": 727.0, "evalue": 2.68e-268, "description": "Internal stop codon at 605321, 606128, 606263. Nonsense mutation around 605321, 606128, 606263.", "psc": { "uniref90_id": "UniRef90_A0A328EGZ1", "product": "AI-2E family transporter", "uniref50_id": "UniRef50_A0A2W4IKW9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EGZ1", "UniRef:UniRef50_A0A2W4IKW9" ] }, "pscc": { "uniref50_id": "UniRef50_A0A2W4IKW9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4IKW9" ], "product": "AI-2E family transporter" } }, "id": "NKOCPLAKPK_1145", "locus": "GCA003260355_01109" }, { "type": "cds", "sequence": "contig_1", "start": 606126, "stop": 606215, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTTATATTATTTTTACAGCGATTATTATCGCGATCTCGATGGAAGGAATGATTCTTTCCTTTGAAAAGAGGCTAAAAACCAGATGA", "aa": "MIYIIFTAIIIAISMEGMILSFEKRLKTR", "aa_hexdigest": "f11bddfa2acc2fb390bdc1310bb0c7e1", "start_type": "ATG", "rbs_motif": "TTTA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3403.213600000001, "isoelectric_point": 9.693869209289552 }, "id": "NKOCPLAKPK_1146", "locus": "GCA003260355_01110" }, { "type": "cds", "sequence": "contig_1", "start": 606778, "stop": 607062, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTTTACTTCGGGCATACTGGCATTTCCAAGGGGATTGATACCTTGGTTGAGGCGATTCCTGAGCTTTTGGAGCAGGATCCTGAACTTCAGCTGATCTTTAATTTGATCCCTGCTCAGAGAGATACCTTTATCAAAGAGAAGATTCAGACTTTTCTCGCTGGTTTACCTCCTTCACAAAAGGGGAGAGTGAAAATTTGGAACGGATTGCCCAAAGAAGAATTGAGAGCTCTTGTTGCAAATGTGCAGGCAGTAGTTGCTCCTTCCTTGTCTGAAGGATTTTGA", "aa": "MLYFGHTGISKGIDTLVEAIPELLEQDPELQLIFNLIPAQRDTFIKEKIQTFLAGLPPSQKGRVKIWNGLPKEELRALVANVQAVVAPSLSEGF", "aa_hexdigest": "73cb81987387be5525af8fef6e60a050", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10398.981999999993, "isoelectric_point": 5.29093723297119 }, "id": "NKOCPLAKPK_1147", "locus": "GCA003260355_01111" }, { "type": "cds", "sequence": "contig_1", "start": 607081, "stop": 607266, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTTTCCAGCTCCCATTTGCAAAATATCACTGTGTTTCGCTATATACCTTGAATGCTTTGAGACTAGTCTATGGAATCCCAGACAAAAAGCTCTTCCTTGCTTATAATGGGGTAGATTATGAGTTCTGGTCTAGAGAAAAGGTGAGTAAGCAAGAGAGTTTGGCGGTGCAAAATAAGTTTTGA", "aa": "MIFQLPFAKYHCVSLYTLNALRLVYGIPDKKLFLAYNGVDYEFWSREKVSKQESLAVQNKF", "aa_hexdigest": "da66589ad7208709720f83ec78f30224", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7222.344500000001, "isoelectric_point": 9.167226982116702 }, "id": "NKOCPLAKPK_1148", "locus": "GCA003260355_01112" }, { "type": "cds", "sequence": "contig_1", "start": 607525, "stop": 607689, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCAAAGACGCTTCTTTACCTCATAGACTATTATCTTCCTCACAAAGGAGGGGTAGAAACCGTTTTTGAGCAGATCATCAGCCGTTCCTTGCAAGAGGGCTACCAAGTCATCGTCCTAACCTCGCACTATAGCCTAGACTTGCCACAAAAAGAACAATCCTGA", "aa": "MPKTLLYLIDYYLPHKGGVETVFEQIISRSLQEGYQVIVLTSHYSLDLPQKEQS", "aa_hexdigest": "64bce5cb506656f3d07220848fecb6aa", "start_type": "ATG", "rbs_motif": "TTATT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6270.122000000001, "isoelectric_point": 5.433262062072752 }, "id": "NKOCPLAKPK_1149", "locus": "GCA003260355_01113" }, { "type": "cds", "sequence": "contig_1", "start": 607865, "stop": 608452, "strand": "-", "frame": 3, "gene": "gspG", "product": "Type II secretion system protein GspG C-terminal domain-containing protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBFD000", "UniRef:UniRef100_A0A328EHA4", "UniRef:UniRef50_A0A2W4ILF6", "UniRef:UniRef90_A0A328EHA4" ], "nt": "ATGAAAAACTTCAAGAAAGGTTTTACCTTAGTGGAAATGCTGATCGTAGTAGTGATCATCGGTATCTTGGCTGCGGCTCTCCTTCCAAGACTTATGTCTGCTCAAGCTTCTGCTAGAGATTCTGCGAGAATGTCAGCTATCCAGCAGATCGCTACAGCTACAGCAGCTTACCTCCAAGAGACAGGGAATTATCCAACAAGCGGTACGGATACAAAGGGATCTACTGATCACTTACTTGCGAAATTGGTAGAAAACGGTAATGTCGCTTCTCTTCCTCAGGAAGCAAAAAAGAATATCTCAAATGAAGCTATAGATGGACCTGCTTTAGTAGGAAAGTATGGATATGCAATTCTCAAGAGAAATGGAATAGCAAATGGAGCAATCGTTTTTGCTGCTAAAGTAGAAAGAGCAGGAAGTGCAAACTATGTATTGGGAGCAGATAAAATTGGTTCAGCGAATATAGATGATGTGACTAAACTTTTTCTTTGTAAGACCGTTACAAAGGGATCTTTGACGCCAGCTTCAGCAACTAATCAGGATTGTAAATATGCAGATGAATCTCAGCTATATTACGTTGGAGTATACTAG", "aa": "MKNFKKGFTLVEMLIVVVIIGILAAALLPRLMSAQASARDSARMSAIQQIATATAAYLQETGNYPTSGTDTKGSTDHLLAKLVENGNVASLPQEAKKNISNEAIDGPALVGKYGYAILKRNGIANGAIVFAAKVERAGSANYVLGADKIGSANIDDVTKLFLCKTVTKGSLTPASATNQDCKYADESQLYYVGVY", "aa_hexdigest": "14535096502e60828ba055264afd12ff", "start_type": "ATG", "rbs_motif": "TTCTT", "ups": { "uniparc_id": "UPI000DBFD000", "uniref100_id": "UniRef100_A0A328EHA4", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBFD000", "UniRef:UniRef100_A0A328EHA4" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EHA4", "uniref90_id": "UniRef90_A0A328EHA4" }, "psc": { "uniref90_id": "UniRef90_A0A328EHA4", "product": "Type II secretion system protein GspG C-terminal domain-containing protein", "uniref50_id": "UniRef50_A0A2W4ILF6" }, "pscc": { "uniref50_id": "UniRef50_A0A2W4ILF6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4ILF6" ], "product": "Prepilin-type N-terminal cleavage/methylation domain-containing protein" }, "genes": [ "gspG" ], "id": "NKOCPLAKPK_1150", "locus": "GCA003260355_01114" }, { "type": "cds", "sequence": "contig_1", "start": 608798, "stop": 609313, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGACAGCTGAAGATCTTGCAGAGAAAACCGTAATAATTAAAGGTCAAAATGTAAATCTTACTGCATATCAGAGCGCTCAGGACGCAAAACCAATCAATCTCTTTATTGACAGAGGAAACCTTTTCCTTCCTAAGGGTGGAAATACAGGGAATATGGCTACCTTTACTCCTTGGGGATATCTCAAAGATGGTGCAGAGGGACCTGAAGATTCAAAGGCTAACTTCTTAAAGGGAAACTTCATTATTAACGGACTGTTCCTTGCTGGAAATAATGGTGAAGATAAGATTAGTAATAAGCTTTATATCCATGGAAAATTGATCTCTTTCAATACCTTTAAAAAGCCAACAGATCAGAGAGTAAGTACTGTTATGGAAATATTGGGAAATTTATGGAAAGCCCCTTACAAAGAGAAAATTGGATTAGATCAGCTCTTTTCATGGGCATGTGTGCTCGGAACGGGAACTGATGGCACAAATTGTAAAGGACTCAGTAAAAATGAAAGGATAATGAGCTGA", "aa": "MTAEDLAEKTVIIKGQNVNLTAYQSAQDAKPINLFIDRGNLFLPKGGNTGNMATFTPWGYLKDGAEGPEDSKANFLKGNFIINGLFLAGNNGEDKISNKLYIHGKLISFNTFKKPTDQRVSTVMEILGNLWKAPYKEKIGLDQLFSWACVLGTGTDGTNCKGLSKNERIMS", "aa_hexdigest": "69e730bbc10de1a93b2f2d10632efb6c", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18740.196899999995, "isoelectric_point": 8.798854637145997 }, "id": "NKOCPLAKPK_1151", "locus": "GCA003260355_01115" }, { "type": "cds", "sequence": "contig_1", "start": 609332, "stop": 609604, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGATCCAGGAAGTGCAAAAACGACGATTTTTAATCTCGGAATTAAGGGAATCATTGGGCTTTCTAATGAAATTAATAATGAAGAGAAAAAATTCTTTGAAGGAAACTCTCTTGAGTCAACGCTTCTTCTCAGAACGGATACCACAATCTCTCAAGTAGTAAATTTGGTTCGTAAAAATGCTGAGAATCTCTGTAGAGGAAAGTGGAAGGAGGAGTGGATACATAGAGATCCAAATGCTGATGTTATCTGTATTGATAATACCAAAAATTGA", "aa": "MDPGSAKTTIFNLGIKGIIGLSNEINNEEKKFFEGNSLESTLLLRTDTTISQVVNLVRKNAENLCRGKWKEEWIHRDPNADVICIDNTKN", "aa_hexdigest": "f4c45c44ddb6d817e8db93febe430c17", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10161.394499999993, "isoelectric_point": 5.785550117492675 }, "id": "NKOCPLAKPK_1152", "locus": "GCA003260355_01116" }, { "type": "cds", "sequence": "contig_1", "start": 609635, "stop": 609898, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAGAAAATGGTGGTCTGAGATGTAATCTTCAGAGATTAAATAACTCTTATCCATTCGGATATTACTATGATACCTATGGACATATAGGAATGATCGGAGCAAAAATTACAAAATCTGGAATGGGAAATCCTAATAAGGTTAATAACTTCCACTCCGGATTAAATAGCTTGTTAGATGAGGGGGTTTGTATTAATAAAGTATTTGATTACAATGGAAGAGAACTTAACTTCAACGATAAAAAAACACTCACAGGTTTCTGA", "aa": "MQENGGLRCNLQRLNNSYPFGYYYDTYGHIGMIGAKITKSGMGNPNKVNNFHSGLNSLLDEGVCINKVFDYNGRELNFNDKKTLTGF", "aa_hexdigest": "56140ba0969d83cdf1394719323afa28", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9789.88349999999, "isoelectric_point": 8.599776268005371 }, "id": "NKOCPLAKPK_1153", "locus": "GCA003260355_01117" }, { "type": "cds", "sequence": "contig_1", "start": 610199, "stop": 610543, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGACAAGTGGAATAGATCAAGGAGTATTCCAGATAAAATTGAAAGGTGTAAATCCTCAAAGTGACTCTACAATAGCAAAAGTAATATTGTCAGAAAAGGCTCTTGATGATAAAGATAAAGATAAGTGGCAAGGACTTACAGGAACAAATGGAAAATGGAATAAAAATAACAGTAGCCTACGAGGTAAGGGATTGACTGGATTAAATTTTGAGTTGTCTGCAAAATTTTTGGATGAATACTTCTCAGTTGCTCACTTAAATGTAAGAGGAAATGATTTTGGGGCTGGATTCTTTTGGCTCCCTCACGAAGAGATAAAGGATGGAGTAATTATAGAAACCCAGTGA", "aa": "MTSGIDQGVFQIKLKGVNPQSDSTIAKVILSEKALDDKDKDKWQGLTGTNGKWNKNNSSLRGKGLTGLNFELSAKFLDEYFSVAHLNVRGNDFGAGFFWLPHEEIKDGVIIETQ", "aa_hexdigest": "08b61c38d8bb472d03d2cf4db36febdd", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12616.037199999992, "isoelectric_point": 6.080032920837401 }, "id": "NKOCPLAKPK_1154", "locus": "GCA003260355_01118" }, { "type": "cds", "sequence": "contig_1", "start": 610652, "stop": 610936, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCTAAAAGTTGATGAGAATTTGTTTGGTGAGGGTGCATTGGATTATATCGCGGGAAAGCCAGGACGAGGAAATAAAACGCTTGATATTAGTAAAAACTATATACCGCTCAAGGAGTTGGCGACAAGCAAGATTTCTGTGTGGCTTCCTAATCCTACGACAAATAAAAAGCAACAAAGAATTACTTTTGTAAATGCCTTTGGAGATGTTATCACCAAAAAAGGTAAATTAAATCCTGGAGAAGAAGTAGAAGTCAGACTTACAAGGCAAATGTTTAACCCTTGA", "aa": "MLKVDENLFGEGALDYIAGKPGRGNKTLDISKNYIPLKELATSKISVWLPNPTTNKKQQRITFVNAFGDVITKKGKLNPGEEVEVRLTRQMFNP", "aa_hexdigest": "bcb7f5091fea5ead481dcdaf120bd467", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10533.061299999994, "isoelectric_point": 9.645388984680174 }, "id": "NKOCPLAKPK_1155", "locus": "GCA003260355_01119" }, { "type": "cds", "sequence": "contig_1", "start": 611024, "stop": 611377, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00560.39", "PFAM:PF12799.13", "PFAM:PF13855.12" ], "nt": "ATGAATAACAAAAGTGTAGCAGCAAATGAACATATCTGTAGTCGAAAAGGAGTTGAATGTAGCTCAGACAAAAAGCAGGTTGTCGGACTTGAACTTCCAGGAAATAAACTTAAAGGAACTTTACCAAACAGTTTTAAAAACCTGATCAATCTGAAGACATTAAATCTAGGAAACAACGAGATTAAAACTTCACTAACAAAAATTAAAAATGAAAAACTTCAGGTTCTCAACCTCGAAAATAACCCATTAAATGAAACTATTCCATCAGATTTCTTTTCTGCAAACAAAGCACTCAAGAATATCAACCTGAAAAATACAAAATTATCAGGAATAATTCCTCAGCTTAATGGATGA", "aa": "MNNKSVAANEHICSRKGVECSSDKKQVVGLELPGNKLKGTLPNSFKNLINLKTLNLGNNEIKTSLTKIKNEKLQVLNLENNPLNETIPSDFFSANKALKNINLKNTKLSGIIPQLNG", "aa_hexdigest": "7bfa08fd19807f320a503c89ea231b42", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00560.39", "name": "LRR_1", "length": 21, "aa_cov": 0.1794871794871795, "hmm_cov": 1.0, "evalue": 7.752114806832972e-12, "score": 39.704444885253906, "start": 72, "stop": 92 }, { "id": "PF12799.13", "name": "LRR_4", "length": 40, "aa_cov": 0.3333333333333333, "hmm_cov": 0.925, "evalue": 3.0857073382744142e-09, "score": 33.33351135253906, "start": 30, "stop": 68 }, { "id": "PF13855.12", "name": "LRR_8", "length": 56, "aa_cov": 0.46153846153846156, "hmm_cov": 1.0, "evalue": 1.2092936765023968e-11, "score": 40.68423080444336, "start": 30, "stop": 83 } ], "seq_stats": { "molecular_weight": 12825.673499999975, "isoelectric_point": 9.698639869689941 }, "id": "NKOCPLAKPK_1156", "locus": "GCA003260355_01120" }, { "type": "cds", "sequence": "contig_1", "start": 611579, "stop": 611935, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13385.13" ], "nt": "ATGATCAATCATCAGTTTACTTGCTTTGTTAATGGTCAAGCTAAAGGAACGGTGCCTCTAGATTCTTATCCAGAACTTGGTCTATTTAACGCAAACATTGGTGCTACAAATGGCAGTCAGAGTGATTTTCAACTCGATGAATTGAAGATATGGAACTATGCACTGAGCACTAGTGAGATTCTTGAAGAATATAAAATCGCTGCTACCACCAAAATGGTAACTATCCAGTACAAAAATAGCTTTGGAGAAACAATTGCTCCACAAGAAGTTTTGAGGAAATACGGAGGATTGGATTTTCAGCTAAACCAGTCTTATACTTATACAGGAAAGCAAATCGCTGGCGCTACAATTACATGA", "aa": "MINHQFTCFVNGQAKGTVPLDSYPELGLFNANIGATNGSQSDFQLDELKIWNYALSTSEILEEYKIAATTKMVTIQYKNSFGETIAPQEVLRKYGGLDFQLNQSYTYTGKQIAGATIT", "aa_hexdigest": "4769131d92e1ac9aff23970a3291befe", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13385.13", "name": "Laminin_G_3", "length": 60, "aa_cov": 0.4830508474576271, "hmm_cov": 1.0, "evalue": 1.5453466671958727e-08, "score": 31.338285446166992, "start": 4, "stop": 60 } ], "seq_stats": { "molecular_weight": 13086.543599999983, "isoelectric_point": 4.967807579040527 }, "id": "NKOCPLAKPK_1157", "locus": "GCA003260355_01121" }, { "type": "cds", "sequence": "contig_1", "start": 612056, "stop": 612265, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGGAGCTAATCATGGAGCGACTGCTTTAGAAGGAAGATTAGGTAAATCGGCTAAGTTTGCAGGAACAGCGAAAATTACTACAGCAAAGCCTATCGAGCTAGATGGAGGAAGGTATTTTACCGTATCTCATTGGATTAAACCAGAAAATATAACTCAGAACTATACAATTGATGCTACAAGTGGAAATAATAAAGGGTTTAAATTCTGA", "aa": "MGANHGATALEGRLGKSAKFAGTAKITTAKPIELDGGRYFTVSHWIKPENITQNYTIDATSGNNKGFKF", "aa_hexdigest": "b59cbb7d4e369f52f3cb6ea72576e4ac", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7401.248100000001, "isoelectric_point": 9.69735050201416 }, "id": "NKOCPLAKPK_1158", "locus": "GCA003260355_01122" }, { "type": "cds", "sequence": "contig_1", "start": 612275, "stop": 612430, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGGAGGAAAAGTAAAAGGGCTTATTTTCAAAGAGCCAACAACAGTAAATGTCGCAAATAATGCCAATATTCAGCTTTTCACAACCTACAAGAAGAAACAAATTCTGAATTATTCTTTTGATGATCCACAAAATCTTGGTCGTAATGCTACCTGA", "aa": "MGGKVKGLIFKEPTTVNVANNANIQLFTTYKKKQILNYSFDDPQNLGRNAT", "aa_hexdigest": "f006f29da65e2b2d290e16a90d715a56", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5702.455500000002, "isoelectric_point": 9.777162361145017 }, "id": "NKOCPLAKPK_1159", "locus": "GCA003260355_01123" }, { "type": "cds", "sequence": "contig_1", "start": 612467, "stop": 612634, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13306.12" ], "nt": "GTGTTGCCAGCCTCTCTAAAAATCATTGGAGAATCAAGTTTTAGGAATAATAAGCTTGCTTCTGTAAATATGACTGGAGCAAATACTATTGAAGAGATTAATGCGCACGTTTTTCAGAATAATAAACTTACATCTATATATTTCCCAGATTCACTCAATAAAATTTGA", "aa": "MLPASLKIIGESSFRNNKLASVNMTGANTIEEINAHVFQNNKLTSIYFPDSLNKI", "aa_hexdigest": "c7fd93ac07508ac19f34a2af28bee29d", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13306.12", "name": "LRR_5", "length": 55, "aa_cov": 0.9818181818181818, "hmm_cov": 0.9636363636363636, "evalue": 2.852870215090463e-10, "score": 36.627262115478516, "start": 2, "stop": 55 } ], "seq_stats": { "molecular_weight": 6095.9112000000005, "isoelectric_point": 8.161778068542482 }, "id": "NKOCPLAKPK_1160", "locus": "GCA003260355_01124" }, { "type": "cds", "sequence": "contig_1", "start": 613358, "stop": 613504, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13306.12" ], "nt": "ATGGAAAGGGGAGTGGAGTCCGTTGTATTCCCAGAAACGCTTATCAAGATTAACAGGGAGGCCTTTATTATGAATAGGCTAACAGAGATTTCCCTCCCTAATAGTCTTCTTTCTATCGAAGAAAGAGCTTTCGCTTATAATCCATGA", "aa": "MERGVESVVFPETLIKINREAFIMNRLTEISLPNSLLSIEERAFAYNP", "aa_hexdigest": "5d0e034234f3d7724f482fc4be24ea91", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13306.12", "name": "LRR_5", "length": 42, "aa_cov": 0.8541666666666666, "hmm_cov": 1.0, "evalue": 1.0443191846795537e-08, "score": 31.573413848876953, "start": 5, "stop": 45 } ], "seq_stats": { "molecular_weight": 5539.341999999999, "isoelectric_point": 4.860040855407715 }, "id": "NKOCPLAKPK_1161", "locus": "GCA003260355_01125" }, { "type": "cds", "sequence": "contig_1", "start": 613586, "stop": 613729, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTAAAAGGGGTGATTAATGGAGATCCTTCAAAAGTCCAAGTTGATCCGACTGCAAAGATTAAAATTGTAAGAAGGACAGAAGCAACTCCTGATGCTTGTTTTCAGATATCCTCAGGAGGAGTTATTACGAGTTATAACTGA", "aa": "MVKGVINGDPSKVQVDPTAKIKIVRRTEATPDACFQISSGGVITSYN", "aa_hexdigest": "2f1f6f04971e3a60b9d94ef1fa1f1a25", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4992.6849, "isoelectric_point": 9.033712959289552 }, "id": "NKOCPLAKPK_1162", "locus": "GCA003260355_01126" }, { "type": "cds", "sequence": "contig_1", "start": 614720, "stop": 614950, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF05725.18", "PFAM:PF13306.12" ], "nt": "ATGACAAATAAGCTAAGTTCTGTCGTTATTCCAAGTGGAGTAAAAGTAATTGCTAATCATGCCTTCAGAAATAACCAAATTTCATCACTTACACTACCAGATACACTAACACATATTAATCTTCGAGCATTCCAAGCAAATAAACTCACATCAGTAGAGATCCCAACTTCTGTTACGACAATTAGCACGTATGCTTTCAATAGTAATCCTGGACTTTCGTCCTTAGGATGA", "aa": "MTNKLSSVVIPSGVKVIANHAFRNNQISSLTLPDTLTHINLRAFQANKLTSVEIPTSVTTISTYAFNSNPGLSSLG", "aa_hexdigest": "09de7d3e2f0e5745a4153680919cbacd", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF05725.18", "name": "FNIP", "length": 38, "aa_cov": 0.5, "hmm_cov": 1.0, "evalue": 3.500216375683815e-06, "score": 23.557985305786133, "start": 4, "stop": 41 }, { "id": "PF13306.12", "name": "LRR_5", "length": 72, "aa_cov": 0.9342105263157895, "hmm_cov": 1.0, "evalue": 2.4878284539209134e-18, "score": 62.677764892578125, "start": 4, "stop": 74 } ], "seq_stats": { "molecular_weight": 8100.1353999999965, "isoelectric_point": 9.992099952697757 }, "id": "NKOCPLAKPK_1163", "locus": "GCA003260355_01127" }, { "type": "cds", "sequence": "contig_1", "start": 615062, "stop": 615307, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13306.12" ], "nt": "GTGACCCCTCCGCTTCCAGAGGGAGAGCGTTGCTTCGACTTTAATTCTGGAACGATCACCAACTACAAGAAGGGAGCATATTCTGAATGTGGAAATAATGTAACTATTCCTTCTACGATCGGAGGAAAGTCTGTAACTGCTATTGGAGATAATGCATTTAAAAACAAGAGCATTACTTCCGTAGTAATCCCAAATTCTGTAACAAGCATTGGAGTAACTGCATTTCAGTATAATCAGTTGACATGA", "aa": "MTPPLPEGERCFDFNSGTITNYKKGAYSECGNNVTIPSTIGGKSVTAIGDNAFKNKSITSVVIPNSVTSIGVTAFQYNQLT", "aa_hexdigest": "3a718c22c2090c78b1a81164f6ed74b3", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13306.12", "name": "LRR_5", "length": 68, "aa_cov": 0.8271604938271605, "hmm_cov": 0.9411764705882353, "evalue": 6.592556468537724e-11, "score": 38.68373489379883, "start": 13, "stop": 79 } ], "seq_stats": { "molecular_weight": 8564.514899999998, "isoelectric_point": 7.752633857727051 }, "id": "NKOCPLAKPK_1164", "locus": "GCA003260355_01128" }, { "type": "cds", "sequence": "contig_1", "start": 615371, "stop": 615862, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACAAAAGAAAAGGAAGAATTTTTATGACTTCATGGTTAGGAAAAACACTCTTGGGAGCAGGAGTAGCATTCTTGCTAGGAAGTGGAATTTTTAGCACGGTATCAGCGCAGACAGCACCTTACTATATCATAGATAGTAATGATAGCCGTTATCCTCAGGGATGTAATACTCCTGGGCTTAGTAATCCAAGTCAATACTGTATTCTTGGGATTCCTTCGGGATCGACAAAAATCCTTATTGCTCCAGCTAATACTGAAATAGCAGGAGATCAAACAGCAGCTTTTAATCACTGTCGTAGTCTGAATATTGCTGGATTTTCTCGAAAAATGATGGAGAACTACGGAGGGTTAAGAGAATGGAATCATGCTTATGTTAATAGAGGAAAATTGGTTAGATTTAACGCAACAGCGGCAGGACAGCAAAAATCAAACCATTATAGAGTCACAAATTCTACAGCATATAGCCATAACTGTACTTTATCGAGTTGA", "aa": "MNKRKGRIFMTSWLGKTLLGAGVAFLLGSGIFSTVSAQTAPYYIIDSNDSRYPQGCNTPGLSNPSQYCILGIPSGSTKILIAPANTEIAGDQTAAFNHCRSLNIAGFSRKMMENYGGLREWNHAYVNRGKLVRFNATAAGQQKSNHYRVTNSTAYSHNCTLSS", "aa_hexdigest": "d13731e2b78e11e6f0f96e81df588c81", "start_type": "ATG", "rbs_motif": "TTATT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17706.8331, "isoelectric_point": 9.766911888122557 }, "id": "NKOCPLAKPK_1165", "locus": "GCA003260355_01129" }, { "type": "cds", "sequence": "contig_1", "start": 616100, "stop": 616231, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01595.25" ], "nt": "ATGGATCCCCGATCGTTAGTCCTCTTTGGTATTTTGATTTTATTATCAATGTTTTTCTCTGGGAGTGAGACTGCGTTTACTGCGGTTCCTCTTCACAAGGTCAATGCACTTAGGAAACAAAAAAAAAGCTGA", "aa": "MDPRSLVLFGILILLSMFFSGSETAFTAVPLHKVNALRKQKKS", "aa_hexdigest": "08bfaf98a475597d6f040e3a57b35039", "start_type": "ATG", "rbs_motif": "TTTA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01595.25", "name": "CNNM", "length": 33, "aa_cov": 0.7674418604651163, "hmm_cov": 1.0, "evalue": 2.9554844775850273e-08, "score": 30.004833221435547, "start": 10, "stop": 42 } ], "seq_stats": { "molecular_weight": 4792.708000000001, "isoelectric_point": 10.446408653259276 }, "id": "NKOCPLAKPK_1166", "locus": "GCA003260355_01130" }, { "type": "cds", "sequence": "contig_1", "start": 616271, "stop": 616888, "strand": "+", "frame": 2, "gene": null, "product": "Hemolysin", "db_xrefs": [ "KEGG:K03699", "SO:0001217", "UniRef:UniRef50_A0A2W4ILD1", "UniRef:UniRef90_A0A328EGM1" ], "nt": "ATGCTGATTGCGATTCTCATTGGGAATAACATTGTAAACATTGCGGCTGCCTCACTAGCGACACTTATTTCACTTCAGATTGCAACGGTAATCAATTATGATCAAGGCACGATTATCACAATTTCTACGATTATCGTTACGATATTGGTCTTGCTCTTTGGTGAAATTTTTCCCAAGACCTTTGCAACGAGTCACGCGGAAAGGATTGCTCTTGGTATTGCTCCGATTTATCTGGTACTGATTAAAGTGTTTTTTCCGATTATCGTCGTTATTGAGCGGATGATGAAAGGGCTTACCAAAAAGGCGAAGAAAAATGCTGTCAGTGAATCAGATTTAGAAGCTTTTATTGAGCTTTCTAAACAAGCGGGGATGTTTGATAACAAGCAGGATCAGAAGATCAAGAAGCTTTTGGCGCTTGATGATTTGACTGCTGAGGAGATTATGACGCCGAGGATCAAGATCAAGGCGATTAATGACGATCATACTTTGGAAGAGGCGATTCAGGTTTTGACGGAGTATCATTATTCTCGTATCCCTGTTTATCATGGGACGATTGATACTATTGATAGGGTGATCACGCTAAAAGAACTCCTTAGAATGACCAAAAAATATCAGTGA", "aa": "MLIAILIGNNIVNIAAASLATLISLQIATVINYDQGTIITISTIIVTILVLLFGEIFPKTFATSHAERIALGIAPIYLVLIKVFFPIIVVIERMMKGLTKKAKKNAVSESDLEAFIELSKQAGMFDNKQDQKIKKLLALDDLTAEEIMTPRIKIKAINDDHTLEEAIQVLTEYHYSRIPVYHGTIDTIDRVITLKELLRMTKKYQ", "aa_hexdigest": "ef553445504689835acfeeede3e46075", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 616100, "stop": 617191, "observations": { "mutation": [ 616229, 616886, 617015, 617123, 617129 ], "stop": [ 616229, 616886, 617015, 617123, 617129 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EGM1", "query_cov": 1.0, "subject_cov": 0.47453703703703703, "identity": 0.995, "score": 370.0, "evalue": 9.63e-126, "gene_start": 1, "gene_end": 205, "reference_start": 58, "reference_end": 262, "reference_sequence": "MDPRSLVLFGILILLSMFFSGSETAFTAVPLHKVNALRKQKKSGSKVLSQLKRQPERMLIAILIGNNIVNIAAASLATLISLQIATVINYDQGTIITISTIIVTILVLLFGEIFPKTFATSHAERIALGIAPIYLVLIKVFFPIIVIIERMMKGLTKKAKKNAVSESDLEAFIELSKQAGMFDNKQDQKIKKLLALDDLTAEEIMTPRIKIKAINDDHTLEEAIQVLTEYHYSRIPVYHGTIDTIDRVITLKELLRMTKKYQGSTLLSELQISPIIKIPRSQPIDSLLEKFQKTHKHIAVVMDEYGGVDGVVSLEDIIEEVFGEIQDETDEELPLIQKNEDGSGYLCQSYARMDELLTELGIHFEDLNLDQEFEAETLSYFITSHLERYPLAGEEIVLPINPLEEKEEKKSLHLKVLRMKKSIIGEISVAVR" }, "paralog": false, "identity": 0.9807162534435262, "score": 671.0, "evalue": 1.14e-246, "description": "Internal stop codon at 616229, 616886, 617015, 617123, 617129. Nonsense mutation around 616229, 616886, 617015, 617123, 617129.", "psc": { "uniref90_id": "UniRef90_A0A328EGM1", "product": "Hemolysin", "uniref50_id": "UniRef50_A0A2W4ILD1", "kegg_orthology_id": "K03699", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EGM1", "UniRef:UniRef50_A0A2W4ILD1", "KEGG:K03699" ] }, "pscc": { "uniref50_id": "UniRef50_A0A2W4ILD1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4ILD1" ], "product": "HlyC/CorC family transporter" } }, "id": "NKOCPLAKPK_1167", "locus": "GCA003260355_01131" }, { "type": "cds", "sequence": "contig_1", "start": 617156, "stop": 617278, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATGAATTACTCACAGAGCTCGGCATTCATTTTGAAGATCTCAATTTAGATCATGAATTTGAAGCGGAAACCTTAAGCTATTTCATCACCAGTCATCTCGAGAGATACCCTCTTGCCTGA", "aa": "MDELLTELGIHFEDLNLDHEFEAETLSYFITSHLERYPLA", "aa_hexdigest": "0ebe7f29c003bc0f4c501a879e77834f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4753.210099999999, "isoelectric_point": 4.175358390808105 }, "id": "NKOCPLAKPK_1168", "locus": "GCA003260355_01132" }, { "type": "cds", "sequence": "contig_1", "start": 617856, "stop": 618170, "strand": "-", "frame": 2, "gene": null, "product": "DnaB-like helicase C-terminal domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A927BAJ0" ], "nt": "GTGATCATTGATTACCTCCAACTGATGCATGGAACAGTCTTTGTTGGGAATAGAGTCCAAGAGATTTCCGAGATCTCAAGAGGGCTAAAAGAGCTTGCAAGGGAGCTTGAAGTGCCGATTATCGCACTTTCTCAACTTTCTCGTGCGGTAGAGCAGAGGGTAGACAAGAAGCCACAACTTTCCGACCTGAGAGAATCAGGAGCGATTGAGCAAGATGCCGATGCGGTTTTGATGCTGCATAGAGAAGACTACTACGATCCTGATACTGATAAGAAAGGTGCAACCGACGTCTGCATCAGAAAAAACAGAAACTGA", "aa": "MIIDYLQLMHGTVFVGNRVQEISEISRGLKELARELEVPIIALSQLSRAVEQRVDKKPQLSDLRESGAIEQDADAVLMLHREDYYDPDTDKKGATDVCIRKNRN", "aa_hexdigest": "fd040f4281b74ff3d005c23c84ccdf96", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0AAN0NBU9", "query_cov": 0.8173076923076923, "subject_cov": 0.8173076923076923, "identity": 0.682, "score": 110.0, "evalue": 1.19e-29, "valid": false, "product": "DnaB-like helicase C-terminal domain-containing protein", "ec_ids": [ "5.6.2.3" ], "uniref50_id": "UniRef50_A0A927BAJ0", "kegg_orthology_id": "K02314" }, "pscc": { "uniref50_id": "UniRef50_A0A927BAJ0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A927BAJ0" ], "product": "DnaB-like helicase C-terminal domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_1169", "locus": "GCA003260355_01133" }, { "type": "cds", "sequence": "contig_1", "start": 618180, "stop": 618482, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03796.21" ], "nt": "ATGGGTAAGACCTCTTTTGCCCTCAATGTGCTGACCGATATTGCTCTCAAACAGAAAAAATCTGCCGTGATGATTTCCCTGGAAATGAGCTCAGAATCTATCGTTGATAGGATTATGTCGGAGGTTTCTAGCGTTCCGATGTATAAAATCACCAAAGGGGACCTAGATAATGAGGATTTTGCGAGAATGGGGGAGGCGATGGAGATCCTTGGAGAAGCTCAGATTTATATTGATGATAAGGGGGCTTTGACCGTTCCTGAGCTCAAATCTAAACTCAGAAAATTAAAAATAGAAAAAGGCTGA", "aa": "MGKTSFALNVLTDIALKQKKSAVMISLEMSSESIVDRIMSEVSSVPMYKITKGDLDNEDFARMGEAMEILGEAQIYIDDKGALTVPELKSKLRKLKIEKG", "aa_hexdigest": "e3beb013da1b0b1197b53708f4bfb27a", "start_type": "ATG", "rbs_motif": "TTCTT", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03796.21", "name": "DnaB_C", "length": 98, "aa_cov": 0.98, "hmm_cov": 1.0, "evalue": 2.6216641279357705e-25, "score": 85.68070220947266, "start": 1, "stop": 98 } ], "seq_stats": { "molecular_weight": 11069.859899999992, "isoelectric_point": 5.48038158416748 }, "id": "NKOCPLAKPK_1170", "locus": "GCA003260355_01134" }, { "type": "cds", "sequence": "contig_1", "start": 618513, "stop": 618800, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAGAGAAAAGTCGCTTTTGAGGCAAGTTTTGAAAGTTTGTCAGGGAATCTCAGGTGAAGTCTACGAACAAAAAGACACGATTCAGATTTTTGATGCTATTGAAAAGAAAATCTTTGACCTTACGCAAAACCAAGTCGGAGACAGCATTATTTCTATTGGAGATATTCTGAAAGGGAGAATAGAGGAGTATATGGAAATCGTGGACAACCCTGAGAAATTCAACGAAAAGAAGGTGATGTCGGGGTATAAGGGGATTGATGGGATGCTGACGGGATTCAAGCCCTGA", "aa": "MREKSLLRQVLKVCQGISGEVYEQKDTIQIFDAIEKKIFDLTQNQVGDSIISIGDILKGRIEEYMEIVDNPEKFNEKKVMSGYKGIDGMLTGFKP", "aa_hexdigest": "8f0388558e1f0509b3fea39453a9c8a1", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10830.421199999993, "isoelectric_point": 5.155546760559081 }, "id": "NKOCPLAKPK_1171", "locus": "GCA003260355_01135" }, { "type": "cds", "sequence": "contig_1", "start": 618816, "stop": 619037, "strand": "-", "frame": 2, "gene": null, "product": "DNA helicase DnaB-like N-terminal domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7W4HL55", "UniRef:UniRef90_A0A7W4HL55" ], "nt": "ATGTGGATTTATGATAGTGATAAACTGGGCTACAAAGATTTCTATCAAAAGGAGCATGGATTTATTTACGAGGCGATTCAGCAGCTCTGGACTGCAAGAAAGACGATAGATGTCGTTACCGTTTCTGATCAGCTTTCAAAAAATGGAAATCTGGATGTGATCGGAGGAACGGACTACCTTTACGAGCTTTCTACCTTCCTTTTCTCTACTCAGCCTTGCTGA", "aa": "MWIYDSDKLGYKDFYQKEHGFIYEAIQQLWTARKTIDVVTVSDQLSKNGNLDVIGGTDYLYELSTFLFSTQPC", "aa_hexdigest": "f12c2e5700eaedc65992cbfb32cd5860", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 618831, "stop": 619115, "observations": { "mutation": [ 619061 ], "stop": [ 619061 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A7W4HL55", "query_cov": 0.9315068493150684, "subject_cov": 0.6355140186915887, "identity": 0.897, "score": 129.0, "evalue": 2.6e-37, "gene_start": 1, "gene_end": 68, "reference_start": 29, "reference_end": 96, "reference_sequence": "MPENINLPPHNIEAEKGVISGVLLDSEVMWIYDSDKLGYKDFYQKEHSYIYEAIQQLRMARKTIDVVTVSDQLSKNGNLDVIGGVDYLYDLSSFLFLRNRVRSIVKL" }, "paralog": false, "identity": 0.8936170212765957, "score": 172.0, "evalue": 5.84e-57, "description": "Internal stop codon at 619061. Nonsense mutation around 619061.", "psc": { "uniref90_id": "UniRef90_A0A7W4HL55", "product": "DNA helicase DnaB-like N-terminal domain-containing protein", "uniref50_id": "UniRef50_A0A7W4HL55", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A7W4HL55", "UniRef:UniRef50_A0A7W4HL55" ] }, "pscc": { "uniref50_id": "UniRef50_A0A7W4HL55", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7W4HL55" ], "product": "DNA helicase DnaB-like N-terminal domain-containing protein" } }, "id": "NKOCPLAKPK_1172", "locus": "GCA003260355_01136" }, { "type": "cds", "sequence": "contig_1", "start": 619178, "stop": 619363, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATTTTTGTCATCACGATTCCGACTTGTTGCGAGGGAGATCCTGTCCTGGAAATGGATTGGATTATCATCTGGATTGCCACGCTCCACTTCGTTTCGCTCGCAATGACGGAATGCGGAAATCAGTCATCGCGAACGAAGTGTGGCGATCCATTAGCAATAAAACTGGATTGCTTCGTCTATAA", "aa": "MDFCHHDSDLLRGRSCPGNGLDYHLDCHAPLRFARNDGMRKSVIANEVWRSISNKTGLLRL", "aa_hexdigest": "311ad26933f6838219906303a44ba221", "start_type": "ATG", "rbs_motif": "TTTTTA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6966.8661999999995, "isoelectric_point": 8.635169410705569 }, "id": "NKOCPLAKPK_1173", "locus": "GCA003260355_01137" }, { "type": "cds", "sequence": "contig_1", "start": 619468, "stop": 619998, "strand": "-", "frame": 1, "gene": null, "product": "ClbS/DfsB family four-helix bundle protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A3DFG6", "UniRef:UniRef90_A0A328EH89" ], "nt": "ATGCCAAGACCAACAAACAAATCAGACTTACTTCAAGCCGCAGAAATGCAGTTTCAGAAGCTTCAAAATCTGATAACTTCTCTTTCAGAACAAGCCCAAGTGTCAGACTTTTCTTTTGATGAAGGGTTTTTAGCTCGCCAGAAGGAAGCACATTGGCAAAGAGATAAAAATCTGAGAGATGTTCTTATCCATCTTTATGAATGGCATCAACTCTTGCTTCATTGGATTGGAGAAAACCAAAAAGGAGTCAAGACCTCATTTCTCCCCGCTCCTTATACTTGGAAAACATATGGAGAAATGAATAGAGCATTGCGAAAAAAGCATCAAACAACTTCGCTTCAAACCACAAAACAGTTTCTAAACAAGAGTCATACTGAGGTAATGAAGCTCATAGATAGCTTTTCAGATCAGGAACTCTTTACAAAAGCTTATTTTTCTTGGACTGGGACAACAAGTTTGGGGTCTTACTGTATTTCTGCGACCTCAAGCCATTACGATTGGGCGATAAAGAAACTGAAGAAAGCTTTTTGA", "aa": "MPRPTNKSDLLQAAEMQFQKLQNLITSLSEQAQVSDFSFDEGFLARQKEAHWQRDKNLRDVLIHLYEWHQLLLHWIGENQKGVKTSFLPAPYTWKTYGEMNRALRKKHQTTSLQTTKQFLNKSHTEVMKLIDSFSDQELFTKAYFSWTGTTSLGSYCISATSSHYDWAIKKLKKAF", "aa_hexdigest": "957243575c7b9b81b8b5138051a85d90", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A328EH89", "query_cov": 1.0, "subject_cov": 0.9832402234636871, "identity": 1.0, "score": 352.0, "evalue": 5.07e-123, "valid": true, "product": "ClbS/DfsB family four-helix bundle protein", "uniref50_id": "UniRef50_A3DFG6" }, "pscc": { "uniref50_id": "UniRef50_A3DFG6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A3DFG6" ], "product": "ClbS/DfsB family four-helix bundle protein" }, "genes": [], "id": "NKOCPLAKPK_1174", "locus": "GCA003260355_01138" }, { "type": "cds", "sequence": "contig_1", "start": 620028, "stop": 620681, "strand": "-", "frame": 2, "gene": null, "product": "Nucleotidyl transferase domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EK69", "UniRef:UniRef90_A0A328EK69" ], "nt": "ATGCATGAACTTCCAGCAGGCTTCATCTGCCCATCTACGAGAGAAAAACCTTGGGGAACCGGGCATGCCGTCCTCGCTGCTAAAGACTTGATTGATGGACCTTTTGCTGTGATCAATGCTGATGATTTCTATGGTAGGGAGAGTTATCAGGTCTTGGCTAACTTTTTGAAGACTCATAATCAAAACTGTATCGTCGGCTACCAACTTTCAGAAGTACTTTCAGACTTTGGAACAGTATCTAGGGGGATCTGCGAACTTGATGAAAATAATAATCTCAAAGCCATTCACGAAACCAAAAAAATCTTCAAATCAGAAGGAAAAATCCTCGCTGAAAACCCTGATGGAACGACTTATGAACTCAATCCTCAGGATCTTTGTTCTATGAATATGATGGGATTTACCAAAGCAAGTCTTGAATTTTACGAAAAATACTTCATTGATTTTTTAGAAAAGAATCTCAACGAACCAAAAACTGAATTTTATATGCCGGAAGTCATCACGAGACTAATTAACGAGGAAGGAGAGTCCGTTCCTGTTTTGCCGACTACTGCAAAATGGTTTGGAGTGACGTATCAGGAAGACAAAGCGGCAACAAGTAGTAATATTGATCAGCTTATTGAGCAGGGAATCTATCCAAAGCATTTGTGGAATTAA", "aa": "MHELPAGFICPSTREKPWGTGHAVLAAKDLIDGPFAVINADDFYGRESYQVLANFLKTHNQNCIVGYQLSEVLSDFGTVSRGICELDENNNLKAIHETKKIFKSEGKILAENPDGTTYELNPQDLCSMNMMGFTKASLEFYEKYFIDFLEKNLNEPKTEFYMPEVITRLINEEGESVPVLPTTAKWFGVTYQEDKAATSSNIDQLIEQGIYPKHLWN", "aa_hexdigest": "42abceb0dfa773023f78fe5e19531e81", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 620028, "stop": 620921, "observations": { "mutation": [ 620699 ], "stop": [ 620699 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EK69", "query_cov": 1.0, "subject_cov": 0.7306397306397306, "identity": 0.9590000000000001, "score": 439.0, "evalue": 1.31e-154, "gene_start": 1, "gene_end": 217, "reference_start": 81, "reference_end": 297, "reference_sequence": "MKLTLVILAAGMGSRYGGLKQLDTFGAGEHALMEYSVFDAKKAGFEKVVFVIRKDFETEFKSRIGSRVEQEIEVSYAFQDMHELPAGFICPSTREKPWGTGHAVLAAKDLIDGPFAVINADDFYGRESYQVLADFLKSHNQNCIVGYQLSEVLSDFGTVSRGMCELDNKGNLKAIHETKKIFKSEGKILAENPDGSAYELNPQDLCSMNMMGFTKASLEFYEKYFIDFLKKNLNEPKTEFYMPEVITRLINEEGESVPVLPTTAKWFGVTYQEDKAATSSNIDQLIEQGIYPKHLWN" }, "paralog": false, "identity": 0.9562289562289562, "score": 581.0, "evalue": 3.67e-213, "description": "Internal stop codon at 620699. Nonsense mutation around 620699.", "psc": { "uniref90_id": "UniRef90_A0A328EK69", "product": "Nucleotidyl transferase domain-containing protein", "uniref50_id": "UniRef50_A0A328EK69", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EK69", "UniRef:UniRef50_A0A328EK69" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EK69", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EK69" ], "product": "Nucleotidyl transferase domain-containing protein" } }, "id": "NKOCPLAKPK_1175", "locus": "GCA003260355_01139" }, { "type": "cds", "sequence": "contig_1", "start": 620697, "stop": 620921, "strand": "-", "frame": 2, "gene": null, "product": "Nucleotidyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1C6EI31" ], "nt": "ATGAAACTAACCCTCGTAATCCTCGCCGCAGGAATGGGAAGCCGTTATGGAGGCTTGAAACAGCTTGATACCTTTGGAGCAGGGGAGCATGCCTTGATGGAATACTCCGTTTTTGATGCGAAAAAAGCTGGATTTGAAAAGGTCGTTTTTGTAATCAGAAAAGACTTTGAAATAGAATTTAAAAGCAGAATCGGTTCAAGGATTGAACAGGAAATGGAGGTCTGA", "aa": "MKLTLVILAAGMGSRYGGLKQLDTFGAGEHALMEYSVFDAKKAGFEKVVFVIRKDFEIEFKSRIGSRIEQEMEV", "aa_hexdigest": "ab9b56ed98f3d3268afa48875bfc93b4", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002ED376C1", "query_cov": 0.9864864864864865, "subject_cov": 0.8390804597701149, "identity": 0.616, "score": 87.0, "evalue": 6.17e-21, "valid": false, "product": "NTP transferase domain-containing protein", "uniref50_id": "UniRef50_A0A1C6EI31" }, "pscc": { "uniref50_id": "UniRef50_A0A1C6EI31", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1C6EI31" ], "product": "Nucleotidyltransferase" }, "genes": [], "id": "NKOCPLAKPK_1176", "locus": "GCA003260355_01140" }, { "type": "cds", "sequence": "contig_1", "start": 621029, "stop": 621313, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13428.12", "PFAM:PF13431.12", "PFAM:PF14559.12", "PFAM:PF07719.23", "PFAM:PF13181.12" ], "nt": "TTGGTAGAAAAGGCGATTAACCTTAAGAACGATAATCCAAAATACTACCTCACAATGGTAGAAATCTATTATAATACCGATAGAAATGCAGAAGCACTCGCCTATATGGAAAAAGTAATTAAACTCAGACCAACGAATCCAAAATACCTTTTAACTATCGCAGAACTCTATGAGCAAGTTGGAGACCTGGATAATGCCAAGAAATACTACTTTAACGTCCTAGAATACGAACAAAATAACGAAAAAGCAAAAGCAAAAATCAGAGAGTTTACCAGGGAAATCTAA", "aa": "MVEKAINLKNDNPKYYLTMVEIYYNTDRNAEALAYMEKVIKLRPTNPKYLLTIAELYEQVGDLDNAKKYYFNVLEYEQNNEKAKAKIREFTREI", "aa_hexdigest": "a9e7d0e2ea8a9bcb45573794d2985a8d", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13428.12", "name": "TPR_14", "length": 43, "aa_cov": 0.4574468085106383, "hmm_cov": 1.0, "evalue": 7.910158844581083e-08, "score": 28.900806427001953, "start": 14, "stop": 56 }, { "id": "PF13431.12", "name": "TPR_17", "length": 32, "aa_cov": 0.3404255319148936, "hmm_cov": 1.0, "evalue": 1.283891235904761e-07, "score": 27.949535369873047, "start": 36, "stop": 67 }, { "id": "PF14559.12", "name": "TPR_19", "length": 58, "aa_cov": 0.6170212765957447, "hmm_cov": 1.0, "evalue": 2.2894919112290113e-10, "score": 37.16017150878906, "start": 28, "stop": 85 }, { "id": "PF07719.23", "name": "TPR_2", "length": 25, "aa_cov": 0.26595744680851063, "hmm_cov": 1.0, "evalue": 4.908768294406857e-08, "score": 29.11209487915039, "start": 22, "stop": 46 }, { "id": "PF13181.12", "name": "TPR_8", "length": 33, "aa_cov": 0.35106382978723405, "hmm_cov": 1.0, "evalue": 1.8263558429664292e-11, "score": 39.828128814697266, "start": 14, "stop": 46 } ], "seq_stats": { "molecular_weight": 11265.767599999988, "isoelectric_point": 6.309321403503419 }, "id": "NKOCPLAKPK_1177", "locus": "GCA003260355_01141" }, { "type": "cds", "sequence": "contig_1", "start": 622220, "stop": 622384, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATGAGGCTCGATCCTAATGCAAAGGCTTATGCTGTAGGTTCCGACTGCGGTATGGAAATCGCTTGTCAGGGACTAGGACTTTTACCTAGCGTTGAGATGGAAAGCATCGCCATGCAAGGACTTGAGGGGAGAGACACCGAAAGGTTAAAAGGAAGATGCTGA", "aa": "MMRLDPNAKAYAVGSDCGMEIACQGLGLLPSVEMESIAMQGLEGRDTERLKGRC", "aa_hexdigest": "1cd400805cb161df4efa71c7aa213e29", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5803.715399999999, "isoelectric_point": 4.812637138366699 }, "id": "NKOCPLAKPK_1178", "locus": "GCA003260355_01142" }, { "type": "cds", "sequence": "contig_1", "start": 622538, "stop": 622843, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAACTATCGTAGTGCTTGATTACTTTATGAATAAACACTTCACTATTCTCAATATTCCTGATGAGAGCTATCAAACTTTCACGCATGGAGACAAAATATCTTATCTCGTAGAGGAAAAAGGAACAGAAAAGGAATCTATTGGGATGGTTATTCTCTATCCACTAAAAATCAGTGAAAAAAGAACTGGACAATTCGTAAAAAAACTGACCTCTCAAGAATGTGAGGAGTTTATAAAACAGCAGGAATTTGCTCTCAAGGTTTTCCCGCTTTTCAAAAAAAAATTTAAACAAAGTTTCCCTTGA", "aa": "MQTIVVLDYFMNKHFTILNIPDESYQTFTHGDKISYLVEEKGTEKESIGMVILYPLKISEKRTGQFVKKLTSQECEEFIKQQEFALKVFPLFKKKFKQSFP", "aa_hexdigest": "c530bdb65ce740ab163620bf8dfcd19d", "start_type": "ATG", "rbs_motif": "TTTA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11948.807899999987, "isoelectric_point": 8.626401710510255 }, "id": "NKOCPLAKPK_1179", "locus": "GCA003260355_01143" }, { "type": "cds", "sequence": "contig_1", "start": 623472, "stop": 623660, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCTTGCTCAAGAGAATTTCCCTAAATTCATTATTGTAGGGTATATTAAGGAGGTCCAAAGGGCAAAAAAGAAGGGTTTTTTTATCAAGATTGAAGATATTTCGACGAGTTGGGAATTTTTCACAAAAGATGCGTTAGATTTCCATAAGTTTGATTTGATTATCTTGCATGGAAGCAAAAAAAACTGA", "aa": "MLAQENFPKFIIVGYIKEVQRAKKKGFFIKIEDISTSWEFFTKDALDFHKFDLIILHGSKKN", "aa_hexdigest": "d8d1b4aca788e3535607e235117f9e61", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7350.5369999999975, "isoelectric_point": 9.347609519958496 }, "id": "NKOCPLAKPK_1180", "locus": "GCA003260355_01144" }, { "type": "cds", "sequence": "contig_1", "start": 624006, "stop": 624335, "strand": "-", "frame": 2, "gene": null, "product": "DNA polymerase III subunit alpha", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A948C4T0" ], "nt": "ATGATTGAACCTGCGGCATCGTATTCGTTCAATAAATCCCATTCAGTTTGTTATGCAATGATTGCTTACCAAACAGCGTATCTAAAGGCTCATTTCCCAGTAGAATTTTCTGCGGCATTGATTCGTTCTGTAGAAGAAGACACTGATACCCAGAGTTTTTATATCTCAGAAATCCAAAACAAAGGAATCTCTGTTCTTTCTCCTGATGTGAATGCCTCATTTAACCACGTAGCAGCGATCGGAGATGCGATCAGACTTGGTTTTTTTAGTGTAAAGGGAATCGGTGAAGATGTTGGAGAGGTAATCCAGCAAGAAAGGCAAAAAAACTGA", "aa": "MIEPAASYSFNKSHSVCYAMIAYQTAYLKAHFPVEFSAALIRSVEEDTDTQSFYISEIQNKGISVLSPDVNASFNHVAAIGDAIRLGFFSVKGIGEDVGEVIQQERQKN", "aa_hexdigest": "ca5e349a868de67e177b4aa1493dbd43", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A948C4T0", "query_cov": 0.8623853211009175, "subject_cov": 1.0, "identity": 0.787, "score": 153.0, "evalue": 1.34e-46, "valid": false, "product": "DNA polymerase III subunit alpha", "uniref50_id": "UniRef50_A0A948C4T0" }, "pscc": { "uniref50_id": "UniRef50_A0A948C4T0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A948C4T0" ], "product": "DNA polymerase III subunit alpha" }, "genes": [], "id": "NKOCPLAKPK_1181", "locus": "GCA003260355_01145" }, { "type": "cds", "sequence": "contig_1", "start": 624441, "stop": 624704, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF17657.6" ], "nt": "ATGGAGTTTATTCCGAGATATATTGAGAGAAAAAATGGGAGAGAGCCGGTGGAATACATGACAGAAGAACTTAGAGCAGACCTGACAAAAAAGTATAGTGCAGAAGTAGCAGAACAGGAAAATCAAAAGCTGATTGAAGATCTCTCTCCGATTATGGGATCAACGTACGGAATTGCTATTTATCAGGAGCAGCTTATGTTTCTTGTGCAAGCGATGGCTGGATTTTCACTGGGAGAAGCCGATATGCTCAGAAGAGGAGTCTGA", "aa": "MEFIPRYIERKNGREPVEYMTEELRADLTKKYSAEVAEQENQKLIEDLSPIMGSTYGIAIYQEQLMFLVQAMAGFSLGEADMLRRGV", "aa_hexdigest": "318b3851474e06bd65c728028c5e58a0", "start_type": "ATG", "rbs_motif": "TTTA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF17657.6", "name": "DNA_pol3_finger", "length": 86, "aa_cov": 0.9885057471264368, "hmm_cov": 0.7209302325581395, "evalue": 1.0708462332020991e-20, "score": 70.11857604980469, "start": 2, "stop": 87 } ], "seq_stats": { "molecular_weight": 10039.413499999999, "isoelectric_point": 4.690660667419433 }, "id": "NKOCPLAKPK_1182", "locus": "GCA003260355_01146" }, { "type": "cds", "sequence": "contig_1", "start": 624810, "stop": 625001, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATTTTCTCGGTTTAAGAAATCTCTCTATCATCAAAACCTGTATCAAAATTATAAAAGCTAGACATGACAAAGCCGGCGAGCCTTTACCGGAGATTTTTGAACATTTCCTGCAAACGACTTCATTTCAGCCTAATATTCAAGATGCCTTTACTTACGACAGTGTTTTTAAACTTGGAGATACAACATGA", "aa": "MDFLGLRNLSIIKTCIKIIKARHDKAGEPLPEIFEHFLQTTSFQPNIQDAFTYDSVFKLGDTT", "aa_hexdigest": "6d3181dbd8a5db1d67f9e304110a8dca", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7215.221799999999, "isoelectric_point": 6.0154638290405265 }, "id": "NKOCPLAKPK_1183", "locus": "GCA003260355_01147" }, { "type": "cds", "sequence": "contig_1", "start": 625011, "stop": 625967, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF07733.18" ], "nt": "ATGGACATTCATGAGTTTGAGCTTCGTTATCACGCTTACAAAGGACTAAAAACTAGATTAAAACTGAATCGAGATACCGATGTTCTAGTAAAATTGGTGAAAACTACAGGAAGGGGAGTCTTGGAAAAATCAGTTCCTGAGACCTCACCAGAAGATCTAAAAAAATTTACCTATGAAACCTATACCGAGTTTAAAAAGCAGATTCTCCAAGAACAGGGAGATATAATCCCTTTCGAAAATTCAAAATATAAGCTTGCAAATTATATTGAGAGACTTGAATATGAGCTCAAGGTTATCAGGGAAATGGGATTTAATTCCTACTTTTTGATCGTCTCAGATTATGTAAGATGGGCAAAAAAACAGATGATCGTCGTAGGTCCAGGAAGAGGTTCTGGAGCTGGATCTTTGCTAGCTTGGGCGATTGAGATCACCGATATTGATCCTCTTCCATTTGACCTTCTCTTTGAAAGATTCCTCAATCCTGCGAGAATTTCTATGCCCGATTTTGATATTGATTTTGAGGATACCCAGAGACAAAGAGTGATTGATTATTGTACAGAAAAATATGGTCAAGAAAAGGTCTGTGCAATAGGAACCTTTATGAAAATGGCAACCAAAGCGGCATTCAAAGACTCGGCAAGGGCGGTTGGAGTTCCTTTTGAGATGGCGAATCAGATTTCTAATATTATCCCAGAAAAAAGCTCACTCAAAGAAATTATCTCAAATGCAGAAGAATACGAGGAAATACACAATTATATCGCCACAAACGAAAAGGTAAAATATGCCTTTGACTTGGCATCTAATCTCGAGGGAAATTTGAGACAGCTGGGGGTTCATGCCTGTGGAATTATCATCGCTCCAGAGCCTGTTTCTACCTATACTCCTGCCCAATTTGCAAAAGAAACAGATCATACCGTTGTGAGTCAGTATGATGGACCCACATTAGAACAAATCTGA", "aa": "MDIHEFELRYHAYKGLKTRLKLNRDTDVLVKLVKTTGRGVLEKSVPETSPEDLKKFTYETYTEFKKQILQEQGDIIPFENSKYKLANYIERLEYELKVIREMGFNSYFLIVSDYVRWAKKQMIVVGPGRGSGAGSLLAWAIEITDIDPLPFDLLFERFLNPARISMPDFDIDFEDTQRQRVIDYCTEKYGQEKVCAIGTFMKMATKAAFKDSARAVGVPFEMANQISNIIPEKSSLKEIISNAEEYEEIHNYIATNEKVKYAFDLASNLEGNLRQLGVHACGIIIAPEPVSTYTPAQFAKETDHTVVSQYDGPTLEQI", "aa_hexdigest": "6361581b13b1a61a1f30ecbb943e451d", "start_type": "ATG", "rbs_motif": "TTCTT", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07733.18", "name": "DNA_pol3_alpha", "length": 235, "aa_cov": 0.7389937106918238, "hmm_cov": 0.9914893617021276, "evalue": 3.751767338604296e-85, "score": 282.07647705078125, "start": 83, "stop": 317 } ], "seq_stats": { "molecular_weight": 36374.10260000001, "isoelectric_point": 5.282866096496581 }, "id": "NKOCPLAKPK_1184", "locus": "GCA003260355_01148" }, { "type": "cds", "sequence": "contig_1", "start": 626100, "stop": 626522, "strand": "-", "frame": 2, "gene": null, "product": "Type IV secretion system pilin", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MXY1", "UniRef:UniRef90_A0A660MXY1" ], "nt": "ATGGGACTAGCGTTCTTTGGAGGAGCGAGTGGTATAGCTCTTGCTCAAAATAACGGTCTAAGCCAAGGGAATGTGCAAGTGCTTAATGATCAGTTTGGAATCAACCCAGACAAAACAACGGGAATCAATGCTCCAGGAACAGGACAAGATCAACAAGCAGGATTAATCCAGATTATCAAAAACTTTATCAACTGGACACTAGGAATCCTCGCGCTTATTACCTTGGTACTTCTCCTTTGGGGAGGTTTCCAGATGGTGACTGCAGCTGGAGATGATGGAAAATACAAGGCTGGATTCAAGATTCTCAAACAGGCAGCAATCGGACTTGCCTTCATCGCTCTTGCATGGTTCATGGTAAGTATTATCTTCTGGCTTATTGGAACTGTCGGTGCTAAGACTACAGGCTCAGCAACAAATCCATAA", "aa": "MGLAFFGGASGIALAQNNGLSQGNVQVLNDQFGINPDKTTGINAPGTGQDQQAGLIQIIKNFINWTLGILALITLVLLLWGGFQMVTAAGDDGKYKAGFKILKQAAIGLAFIALAWFMVSIIFWLIGTVGAKTTGSATNP", "aa_hexdigest": "1e064ed8c42f1986e68be48b09c48ca6", "start_type": "ATG", "rbs_motif": "TTATT", "psc": { "uniref90_id": "UniRef90_A0A660MXY1", "query_cov": 1.0, "subject_cov": 0.9333333333333333, "identity": 1.0, "score": 274.0, "evalue": 3.85e-93, "valid": true, "product": "Type IV secretion system pilin", "uniref50_id": "UniRef50_A0A660MXY1" }, "pscc": { "uniref50_id": "UniRef50_A0A660MXY1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MXY1" ] }, "genes": [], "id": "NKOCPLAKPK_1185", "locus": "GCA003260355_01149" }, { "type": "cds", "sequence": "contig_1", "start": 627668, "stop": 627880, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAGTTATAATAATGGACTCGCTGATATTGTGCGTAATGCAGAAACCGAGATTGTCCCACTTTGGGGAGAAGGTGTGATTTATGAAATCTTGGATTTTTCTCAGTTTTCTAACTCTGAATCCGCGAATCAAGAACAAACATCATTAAAATTCAGAGTTTCTCCCTCTTCGTTTTTTCAAACCAATACGCTTGGTGCTCAAAAACTCTTTTGA", "aa": "MSYNNGLADIVRNAETEIVPLWGEGVIYEILDFSQFSNSESANQEQTSLKFRVSPSSFFQTNTLGAQKLF", "aa_hexdigest": "991430751fef2105d3c39b40904e02fa", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7866.605299999998, "isoelectric_point": 4.381058692932128 }, "id": "NKOCPLAKPK_1186", "locus": "GCA003260355_01150" }, { "type": "cds", "sequence": "contig_1", "start": 628145, "stop": 628372, "strand": "+", "frame": 2, "gene": null, "product": "TRAM domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A351Y5Z5" ], "nt": "GTGATTATAGATCCTCCTCGTGAAGGATTACACCCGAATGTCATCAAGTATTTGGCAGACCTAAAAAAACAATATGCATTTAAGTTGCTGTATATTTCTTGCAATCCAATCACAATGGCAAGAGATATTGAACTACTAGTTGACGAAGGTTTTACTTTCAAGCAGGTGCAGGGACTCGACATGTTCCCTCACACACATCATGTTGAATGTATTTCTGTGTTGAGTTAA", "aa": "MIIDPPREGLHPNVIKYLADLKKQYAFKLLYISCNPITMARDIELLVDEGFTFKQVQGLDMFPHTHHVECISVLS", "aa_hexdigest": "ed4fa0f3733de592535b2d8efd015e85", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A948C390", "query_cov": 0.9866666666666667, "subject_cov": 0.8222222222222222, "identity": 0.662, "score": 112.0, "evalue": 4.32e-31, "valid": false, "product": "23S rRNA (Uracil-5-)-methyltransferase RumA", "uniref50_id": "UniRef50_A0A351Y5Z5" }, "pscc": { "uniref50_id": "UniRef50_A0A351Y5Z5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A351Y5Z5" ], "product": "TRAM domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_1187", "locus": "GCA003260355_01151" }, { "type": "cds", "sequence": "contig_1", "start": 628671, "stop": 628967, "strand": "-", "frame": 2, "gene": "yopX", "product": "YopX protein domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EH45", "UniRef:UniRef90_A0A328EH45" ], "nt": "ATGAGGTTTAGACCAAGAGATAAGGTTAAGCTAAACAGTATTAATGAAATTAAAAAATACGGAGGGATTCATGAGCAATATGTAGAATTTATTAATAATAATCAAAACAAAATATTTGTTATAGATCGTATAGACCAAGAAACTAACTCATATATTTTTGAGGGGTATGATCTTTTTTATTTTGATGAGTTTTGCATACTTGGATTAGATCAGCCGAGAATAGGAGATATTATTGAAGTAAAAACAAGTAGTGGATACTGGAAGGAGAGAATCTATATAGCAACAGCGGAAAATTGA", "aa": "MRFRPRDKVKLNSINEIKKYGGIHEQYVEFINNNQNKIFVIDRIDQETNSYIFEGYDLFYFDEFCILGLDQPRIGDIIEVKTSSGYWKERIYIATAEN", "aa_hexdigest": "27afb9549a594252ef0042b2599ff607", "start_type": "ATG", "rbs_motif": "TTTTTA", "genes": [ "yopX" ], "truncated": "5-prime", "pseudogene": { "start": 628581, "stop": 628967, "observations": { "mutation": [ 628673 ], "stop": [ 628673 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EH45", "query_cov": 1.0, "subject_cov": 0.765625, "identity": 1.0, "score": 201.0, "evalue": 4.38e-65, "gene_start": 1, "gene_end": 98, "reference_start": 1, "reference_end": 98, "reference_sequence": "MRFRPRDKVKLNSINEIKKYGGIHEQYVEFINNNQNKIFVIDRIDQETNSYIFEGYDLFYFDEFCILGLDQPRIGDIIEVKTSSGYWKERIYIATAENGEVICINNAYEELYKSERLSPLRLTKRSKN" }, "paralog": false, "identity": 0.9921875, "score": 260.0, "evalue": 7.6e-91, "description": "Internal stop codon at 628673. Nonsense mutation around 628673.", "psc": { "uniref90_id": "UniRef90_A0A328EH45", "product": "YopX protein domain-containing protein", "uniref50_id": "UniRef50_A0A328EH45", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EH45", "UniRef:UniRef50_A0A328EH45" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EH45", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EH45" ], "product": "YopX protein domain-containing protein" } }, "id": "NKOCPLAKPK_1188", "locus": "GCA003260355_01152" }, { "type": "crispr", "strand": "?", "repeats": [ { "strand": "?", "start": 629063, "stop": 629098 }, { "strand": "?", "start": 629126, "stop": 629161 }, { "strand": "?", "start": 629188, "stop": 629223 }, { "strand": "?", "start": 629251, "stop": 629286 }, { "strand": "?", "start": 629314, "stop": 629349 }, { "strand": "?", "start": 629376, "stop": 629411 } ], "spacers": [ { "strand": "?", "start": 629099, "stop": 629125, "sequence": "AATACAAGAGAAACCTCGAAGACGCTC" }, { "strand": "?", "start": 629162, "stop": 629187, "sequence": "AGGCCCACACAGGTGTAGAGCTTAAC" }, { "strand": "?", "start": 629224, "stop": 629250, "sequence": "ATTTTGAAAATATGATTGAGAAAAAAA" }, { "strand": "?", "start": 629287, "stop": 629313, "sequence": "TTATTATACTTTTTCACAGAATAATCA" }, { "strand": "?", "start": 629350, "stop": 629375, "sequence": "TTTTCTTCATATCTGCAAACTGCTAA" }, { "strand": "?", "start": 629412, "stop": 629422, "sequence": "TACGGTATTGC" } ], "sequence": "contig_1", "start": 629063, "stop": 629422, "product": "CRISPR array with 6 repeats of length 36, consensus sequence ATCTACAAAAGTAGAAATCTTATATAAGTTCTAAAC and spacer length 26", "spacer_length": 26, "repeat_length": 36, "repeat_consensus": "ATCTACAAAAGTAGAAATCTTATATAAGTTCTAAAC", "db_xrefs": [ "SO:0001459" ], "nt": "ATCTACAAAAGTAGAAATCTTATATAAGTTCTAAACAATACAAGAGAAACCTCGAAGACGCTCATCTACAAAAGTAGAAATCTTATATAAGTTCTAAACAGGCCCACACAGGTGTAGAGCTTAACATCTACAAAAGTAGAAATCTTATATAAGTTCTAAACATTTTGAAAATATGATTGAGAAAAAAAATCTACAAAAGTAGAAATCTTATATAAGTTCTAAACTTATTATACTTTTTCACAGAATAATCAATCTACAAAAGTAGAAATCTTATATAAGTTCTAAACTTTTCTTCATATCTGCAAACTGCTAAATCTACAAAAGTAGAAATCTTATATAAGTTCTAAACTACGGTATTGC", "id": "NKOCPLAKPK_48" }, { "type": "crispr", "strand": "?", "repeats": [ { "strand": "?", "start": 630326, "stop": 630361 }, { "strand": "?", "start": 630390, "stop": 630425 }, { "strand": "?", "start": 630454, "stop": 630489 }, { "strand": "?", "start": 630517, "stop": 630552 }, { "strand": "?", "start": 630580, "stop": 630615 }, { "strand": "?", "start": 630644, "stop": 630679 } ], "spacers": [ { "strand": "?", "start": 630362, "stop": 630389, "sequence": "CAGAGGAATCTGACTACTTACGACAGAT" }, { "strand": "?", "start": 630426, "stop": 630453, "sequence": "TCATAAATATCATGATCTGACAATCATT" }, { "strand": "?", "start": 630490, "stop": 630516, "sequence": "AATCAAAATATAAAATAAGCGGTATAC" }, { "strand": "?", "start": 630553, "stop": 630579, "sequence": "GAAAAACTGAATCTAATGCTACTTTTG" }, { "strand": "?", "start": 630616, "stop": 630643, "sequence": "TATACCCTTAAGGGGAAATGACCTATGC" }, { "strand": "?", "start": 630680, "stop": 630689, "sequence": "TTGAAACCAG" } ], "sequence": "contig_1", "start": 630326, "stop": 630689, "product": "CRISPR array with 6 repeats of length 36, consensus sequence ATCTACAAAAGTAGAAATCTTATATAAGTTCTAAAC and spacer length 27", "spacer_length": 27, "repeat_length": 36, "repeat_consensus": "ATCTACAAAAGTAGAAATCTTATATAAGTTCTAAAC", "db_xrefs": [ "SO:0001459" ], "nt": "ATCTACAAAAGTAGAAATCTTATATAAGTTCTAAACCAGAGGAATCTGACTACTTACGACAGATATCTACAAAAGTAGAAATCTTATATAAGTTCTAAACTCATAAATATCATGATCTGACAATCATTATCTACAAAAGTAGAAATCTTATATAAGTTCTAAACAATCAAAATATAAAATAAGCGGTATACATCTACAAAAGTAGAAATCTTATATAAGTTCTAAACGAAAAACTGAATCTAATGCTACTTTTGATCTACAAAAGTAGAAATCTTATATAAGTTCTAAACTATACCCTTAAGGGGAAATGACCTATGCATCTACAAAAGTAGAAATCTTATATAAGTTCTAAACTTGAAACCAG", "id": "NKOCPLAKPK_49" }, { "type": "cds", "sequence": "contig_1", "start": 631066, "stop": 631170, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAGGAGAAGAAAACGCTGAAGGTAGATAACTACGATGTTTTCAAGTACAAAAGATATACGGAAGATAAAATCCTTTTTCATGTGCCGATTACCTTGTGA", "aa": "MKKEKKTLKVDNYDVFKYKRYTEDKILFHVPITL", "aa_hexdigest": "7cec64fc5aa71805d08f1de43dbc6da8", "start_type": "ATG", "rbs_motif": "TTCTT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4232.9826, "isoelectric_point": 9.573893547058105 }, "id": "NKOCPLAKPK_1189", "locus": "GCA003260355_01153" }, { "type": "cds", "sequence": "contig_1", "start": 631321, "stop": 631638, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF22222.1" ], "nt": "ATGCTTCCAAAATGTCTAATGCCTGGTTCTGACAAGAAAAAATACGGAGCTTCTGATCAGGTTTTAGACCTTTATGCTAAAGGACGTTTCAAAAAGTCAGAAAAGAGCTTCAATCTCGCAGACCTCCATACTTTAATTGATTTTTATAAGTCTGCACTGCCAAAATATGAGGATTGGAAAGTGTTCAATTTCCAGTTTCAAGCGACAGAAAATTATCAAGATATTTCTCAGTTTTATAGAGAAGTTGAACAACAAGGTTATTTGTTAAACTGGAGAAAGGTTAATGAAGAACTGATTAAACAAGGAATAAAAGACTGA", "aa": "MLPKCLMPGSDKKKYGASDQVLDLYAKGRFKKSEKSFNLADLHTLIDFYKSALPKYEDWKVFNFQFQATENYQDISQFYREVEQQGYLLNWRKVNEELIKQGIKD", "aa_hexdigest": "92e410c43a2eff0acd78035874fa4935", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF22222.1", "name": "Cpf1_PI-like", "length": 87, "aa_cov": 0.8285714285714286, "hmm_cov": 0.9885057471264368, "evalue": 3.7797170334632513e-34, "score": 113.71255493164062, "start": 1, "stop": 87 } ], "seq_stats": { "molecular_weight": 12499.102599999993, "isoelectric_point": 8.57244167327881 }, "id": "NKOCPLAKPK_1190", "locus": "GCA003260355_01154" }, { "type": "cds", "sequence": "contig_1", "start": 631648, "stop": 632046, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTTTGGACAATGCTTTGTATGCATATAGGATGCTCAAGTGGTTCAAAGTAGATACGAGTAAACTTGGATTTGTCCCAGAAGGAGAGTTTTATCCGAGTCTTGATCAGCTTTTGCAGGATTATCCTTTGCCAAAGTGGTATGATATGATCAGGAATTATCTCACGAGAAAACCCTATTCACAAGCAAAATTAAAGCTGAATTTTGACTGTTCTACCTTGCTGAACGGACGAGATAAAAATAAAGAAACACAGAACTTATCCGTGATTTTGAGAAAAGATGGGAAATTTTATCTCGCAATTATGAAAAAGGATCAGAATAAATTTTTTGAAAAATCGGCTCTCTACGAGGGGAATCTCGGGACGATGGAAAAGATGGACTATAAGCTCCTTCCATGA", "aa": "MLLDNALYAYRMLKWFKVDTSKLGFVPEGEFYPSLDQLLQDYPLPKWYDMIRNYLTRKPYSQAKLKLNFDCSTLLNGRDKNKETQNLSVILRKDGKFYLAIMKKDQNKFFEKSALYEGNLGTMEKMDYKLLP", "aa_hexdigest": "a1a91d5e8c604aaf742ba7213029be7a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15703.132199999982, "isoelectric_point": 9.282109642028807 }, "id": "NKOCPLAKPK_1191", "locus": "GCA003260355_01155" }, { "type": "cds", "sequence": "contig_1", "start": 632143, "stop": 632580, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTCACACCATGATTAGAGAAACAGATCAAAGAATCCCAGAATTTAAGAAGCTTTTGGCTCAAATTTTTGAGGAGAAGGCGGATCTTTCACAGATTTTCTTGTCCAAAACAGCTCTCAATATCATTTCTAACCGCTACTTCTCATCTTGGCATACTTTACTAGAAAAGGGAGTTGAGCTCAAGCTTTTTAAGTTCAAAAAAAATGATGAAGAAAGTTTCAAGCTTCCTGCCTATCTTTCGCTCGCTGAACTCAAGGAACTTTTGGAAGCAGTTCCTTTTCAAGCAGCAGAAAAAGCCGATACCGCCGAAGGAAATCATCATCAAGTCTCTCTTCTCAAACTCCAACGAGAAAATCTTCATTTAGAAAATTCACATTCTAACTGGGAACTTCTGCTCAAAAGTATGAAATCTGACTTTGAATCTTTTTGGACTTGA", "aa": "MLHTMIRETDQRIPEFKKLLAQIFEEKADLSQIFLSKTALNIISNRYFSSWHTLLEKGVELKLFKFKKNDEESFKLPAYLSLAELKELLEAVPFQAAEKADTAEGNHHQVSLLKLQRENLHLENSHSNWELLLKSMKSDFESFWT", "aa_hexdigest": "27f142664599eaa92ab5d72dccadcf48", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17023.32539999999, "isoelectric_point": 6.076849937438964 }, "id": "NKOCPLAKPK_1192", "locus": "GCA003260355_01156" }, { "type": "cds", "sequence": "contig_1", "start": 632641, "stop": 632826, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCTTTCACTTTCAGAACTAGAGAAAAAAGTCTTCAATCCTGACTCTTACAGCGAATACCTTACTCAATCAGGAATTGATTTTTACAATGAAATGCTTGCAAATATTAGGTCAAAAGCAAATCTTTATACCCAAGAACATAAAACCAAACTTCCTCAGCCAAAACTCCTTTATAAACAAATCTGA", "aa": "MLSLSELEKKVFNPDSYSEYLTQSGIDFYNEMLANIRSKANLYTQEHKTKLPQPKLLYKQI", "aa_hexdigest": "f03fdd6f8c76bbf58101cec86b4e0324", "start_type": "GTG", "rbs_motif": "TTTTT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7199.174400000002, "isoelectric_point": 7.9651494979858395 }, "id": "NKOCPLAKPK_1193", "locus": "GCA003260355_01157" }, { "type": "cds", "sequence": "contig_1", "start": 633034, "stop": 633531, "strand": "-", "frame": 1, "gene": null, "product": "Cas12a REC1 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7W4HMH4" ], "nt": "ATGACAAAGCTCCTCTTCTCCCAATTCACCAATCTCTATCCCATCCAAAAAACCCTTCGCCGAGAGCTAAAACCGCTCAACGAAAATTTTGAACACGATCCAACTCTTAGTGCGCTCCGAAACTCAGAAATTCCTCAGAGGGATGAACAGAGAGAAAAAGACTATCAGGCAATCAAACCGCTCTTGGACGAGCTTCACAATCAATTTATCACAGAAAGCCTTCAGTCGCTAGAACCCCAGAATCGATCAGATTTTGTCCTTTTTTATCAAACTTACCAAAAAAAGAAAAAGAACAAAGCTGAGCTCTCCGAAAAGGAGCTAAAAAGCCTTGATGAAGAATTTGAAAGCCGCACAAAAGCCTTGAGAACTGCGATTGGAACGAGCTTTTCAAATACTGCAGAGCTCCGAAAAAGCAATCCCGACTATGTCAATGAAAAAGGAAAACCTTTTCTGACTCAAAAAAGTTATAAAATCCTGACTGAAGCTGGAGTGCTTTGA", "aa": "MTKLLFSQFTNLYPIQKTLRRELKPLNENFEHDPTLSALRNSEIPQRDEQREKDYQAIKPLLDELHNQFITESLQSLEPQNRSDFVLFYQTYQKKKKNKAELSEKELKSLDEEFESRTKALRTAIGTSFSNTAELRKSNPDYVNEKGKPFLTQKSYKILTEAGVL", "aa_hexdigest": "6ed8b223bd82fe3da48f01b68ec2669b", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A7W4HMH4", "query_cov": 0.9696969696969697, "subject_cov": 0.8648648648648649, "identity": 0.888, "score": 277.0, "evalue": 2.12e-93, "valid": false, "product": "Cas12a REC1 domain-containing protein", "uniref50_id": "UniRef50_A0A7W4HMH4" }, "pscc": { "uniref50_id": "UniRef50_A0A7W4HMH4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7W4HMH4" ], "product": "Cas12a REC1 domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_1194", "locus": "GCA003260355_01158" }, { "type": "cds", "sequence": "contig_1", "start": 633770, "stop": 634363, "strand": "-", "frame": 3, "gene": null, "product": "glycine--tRNA ligase", "db_xrefs": [ "EC:6.1.1.14", "GO:0000166", "GO:0004820", "GO:0005524", "GO:0006426", "KEGG:K01880", "SO:0001217", "UniRef:UniRef50_A0A2U7PGD8", "UniRef:UniRef90_A0A328EKI8" ], "nt": "ATGGAGATTGAATATTTCGTGCCTGCTGATGCAGAGAAGGCGATGGAGATTTACAATCAATGGAAAGCTGACTGTATGCACTATTGGACTGAAGTAATCGGAATTAGTCCAGAGCATTTGAGATTCAAAGATCATGAAAAGCTGGCTCACTATGCGGCAGCAGCAGTAGATATAGAATACCTCTATCCACGAGGGTTTGGTGAGATTCAGGGAATCCACAACAGAACGAATTTTGATCTTTCTCAGCATCAAGAGTTCAGTAAAAAAGATATGCAATATTCTGATCCCTATACTGGGGAGAGATACATCCCACGATGTGTTGAGGCTTCCTTTGGGCTTGGTCGTCAAGTGATGGTTACGATGCTTGAGTTCTATGATGAGGAAACTCTGGAAGATGGTTCTAGCCGCGTGGTGATTCGTTTCCCTAAGCAGCTCGCTCCTGTGAAGTTTGCGATCCTTCCACTCGTAAAGAAAGATCAAAAGCAGGTTGAAACCGCAGAAAAAATCTTCCGCGATCTCAGTAAGGCTTTCAAATGTGAATATGATGATGGAGGAGCGATCGGAAAAAGATATAGAAGACAAGATGAAATCTGA", "aa": "MEIEYFVPADAEKAMEIYNQWKADCMHYWTEVIGISPEHLRFKDHEKLAHYAAAAVDIEYLYPRGFGEIQGIHNRTNFDLSQHQEFSKKDMQYSDPYTGERYIPRCVEASFGLGRQVMVTMLEFYDEETLEDGSSRVVIRFPKQLAPVKFAILPLVKKDQKQVETAEKIFRDLSKAFKCEYDDGGAIGKRYRRQDEI", "aa_hexdigest": "40d99150d25663be371e292ac12a3f44", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 633635, "stop": 634663, "observations": { "mutation": [ 633772, 634429, 634615 ], "stop": [ 633772, 634429, 634615 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EKI8", "query_cov": 1.0, "subject_cov": 0.4121338912133891, "identity": 1.0, "score": 407.0, "evalue": 1.2e-139, "gene_start": 1, "gene_end": 197, "reference_start": 237, "reference_end": 433, "reference_sequence": "MKTSLETVVAWAKRKGFVYPNSEIYGGLANAWDFGPYGALLKKNIADLWVKHFTQEREDVVFMDTAIIAHPTTWQASGHLASFADALIDDKKTGQRFRADKLIEDYFEKKRNGLSDEELAQKLDVPNLSPESRGNEGMRDFIVKHLPNNPETGNPADWTECRNFNLMLSSHLGPVANETSKVYMRPETCQSLFTDFKAVLDTTRMRIPFGLAQVGKAFRNEITPGQFLYRTREFEQMEIEYFVPADAEKAMEIYNQWKADCMHYWTEVIGISPEHLRFKDHEKLAHYAAAAVDIEYLYPRGFGEIQGIHNRTNFDLSQHQEFSKKDMQYSDPYTGERYIPRCVEASFGLGRQVMVTMLEFYDEETLEDGSSRVVIRFPKQLAPVKFAILPLVKKDQKQVETAEKIFRDLSKAFKCEYDDGGAIGKRYRRQDEIGTPYCITIDPETVDETSPNFGTVTVRERDSMQQRRVKIEELSQCF" }, "paralog": false, "identity": 0.9883040935672515, "score": 695.0, "evalue": 1.63e-255, "description": "Internal stop codon at 633772, 634429, 634615. Nonsense mutation around 633772, 634429, 634615.", "psc": { "uniref90_id": "UniRef90_A0A328EKI8", "product": "glycine--tRNA ligase", "ec_ids": [ "6.1.1.14" ], "uniref50_id": "UniRef50_A0A2U7PGD8", "kegg_orthology_id": "K01880", "go_ids": [ "GO:0000166", "GO:0004820", "GO:0005524", "GO:0006426" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EKI8", "EC:6.1.1.14", "UniRef:UniRef50_A0A2U7PGD8", "KEGG:K01880", "GO:0000166", "GO:0004820", "GO:0005524", "GO:0006426" ] }, "pscc": { "uniref50_id": "UniRef50_A0A2U7PGD8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2U7PGD8" ], "product": "Glycine--tRNA ligase" } }, "id": "NKOCPLAKPK_1195", "locus": "GCA003260355_01159" }, { "type": "cds", "sequence": "contig_1", "start": 634733, "stop": 635071, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAACATCACTAGAAACGGTTGTAGCTTGGGCGAAAAGAAAAGGTTTCGTCTATCCCAATTCAGAAATTTATGGAGGGCTGGCAAATGCTTGGGATTTCGGTCCTTATGGAGCCTTGCTCAAAAAAAATATTGCTGATCTCTGGGTCAAACATTTCACGCAGGAGAGAGAAGATATGGTCTTTATGGATACGGCGATCATTGCACATCCCACGACTTGGCAGGCAAGTGGACATTTGGCTTCTTTCGCTGATGCGTTGATTGATGACAAGAAAACGGGGCAGAGATTTAGAGCGGATAAGTTGATTGAAGATTATTTTGAAAAGAAAAGAAACTGA", "aa": "MKTSLETVVAWAKRKGFVYPNSEIYGGLANAWDFGPYGALLKKNIADLWVKHFTQEREDMVFMDTAIIAHPTTWQASGHLASFADALIDDKKTGQRFRADKLIEDYFEKKRN", "aa_hexdigest": "02d2a57cf86cf2060bc9ab8c0f55c45b", "start_type": "ATG", "rbs_motif": "TTATT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12843.477899999982, "isoelectric_point": 7.954705619812011 }, "id": "NKOCPLAKPK_1196", "locus": "GCA003260355_01160" }, { "type": "cds", "sequence": "contig_1", "start": 635370, "stop": 635720, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGGCTATAGCTCCTCCAATCGCCACAACCAGAAGTCAGATTCAAATAACAGGTCTTACGACAAGCCATGCTATCGCGATGGTTCCTAATCCTAGCACTAGTGTTGCCGCAAAAGCGATAACTCAAGTTCCAAAACCAATAAGTCCTGCGAGAGGAGGAAGCACTTTAGCGAGAGTTGGGATAAACTCGAGCATCATCGCAAATCCCATATACATCAACACAAGTCACACAAACCTTAGTATCCATGTAAGTCCCAGATTTTCAGCTTGTGCATCGGCAAACATTTCAGTAGAGCTTTTCTTGCCATTTGCTAGGAGAGCAAGGTCCACTCCGTTTGAGGTTTTATAG", "aa": "MKAIAPPIATTRSQIQITGLTTSHAIAMVPNPSTSVAAKAITQVPKPISPARGGSTLARVGINSSIIANPIYINTSHTNLSIHVSPRFSACASANISVELFLPFARRARSTPFEVL", "aa_hexdigest": "0799f368e43e7616434c7cc65b71590a", "start_type": "ATG", "rbs_motif": "TTTTT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12160.974699999984, "isoelectric_point": 11.630241584777831 }, "id": "NKOCPLAKPK_1197", "locus": "GCA003260355_01161" }, { "type": "cds", "sequence": "contig_1", "start": 635597, "stop": 635842, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTTATGTTGGCTTGGATTCTCATAGTCCGGCAGTTGGGGACATGAGAATAAGATTCTCCGTGGTAAAAACAGGAACCGTAAGTCTCGTAGGAAAACAATCTGGAAACGAAATAACTCTCTATAAAACCTCAAACGGAGTGGACCTTGCTCTCCTAGCAAATGGCAAGAAAAGCTCTACTGAAATGTTTGCCGATGCACAAGCTGAAAATCTGGGACTTACATGGATACTAAGGTTTGTGTGA", "aa": "MIYVGLDSHSPAVGDMRIRFSVVKTGTVSLVGKQSGNEITLYKTSNGVDLALLANGKKSSTEMFADAQAENLGLTWILRFV", "aa_hexdigest": "a20edcadf4079e353425cb8af0899901", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8719.930899999998, "isoelectric_point": 8.086027717590333 }, "id": "NKOCPLAKPK_1198", "locus": "GCA003260355_01162" }, { "type": "cds", "sequence": "contig_1", "start": 635915, "stop": 636556, "strand": "-", "frame": 3, "gene": null, "product": "Transmembrane protein 43", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EH02", "UniRef:UniRef90_A0A328EH02" ], "nt": "GTGATCGGTTCAATCGTTCTTTTAGCCCGAAATGAAAACCACTTTTTTGTGACGAAGGTAGGACTAAACGAAGGAGAAAAACTTGTGCAAGATGCTGATGCACAGCAGCTTAATCCTGAGCTTGATGGAAAACTCGTTCACATCCAAGGGGTGACCAGCTCTCCTGCATCATCACTCCAAGATCCAAGATTCGGAGTTAAGGCTGATGATCTAAAGCTTATCAGATCCGTAGAGATGTATCAGCGATCAGAATCTTCAAGCGAAGAATGTCATGACAACTATGGTGGAAGTCAGGACTGTACCACAACCTACGACTACAAGAAAAAGTGGTCAGATTCAGCCATTGATTCAAGTAGATTCCATGAGACTCAGGGACACCAAAATCCAGCGACTCGAGAATATGAAAGCGAGACTCGAGAAAAGGAACCAATCATTGTAGGAGCCTTTACTTTAGATAAAGCATTTACCTCTCAGCTTACAGATGATGTCAAGCTTCCTCTAAATGGCCAGGAATTGAAGCTTCCAGCGCAGGAAACAGAATATACAAGCTCGATGAATAACAATACAGAGACCTCTGCTGAAACCTCTGAAATGACAGAGCAACTCTCAGGAGATGCTTCGACAGGAGAGCAAGCGAACTGA", "aa": "MIGSIVLLARNENHFFVTKVGLNEGEKLVQDADAQQLNPELDGKLVHIQGVTSSPASSLQDPRFGVKADDLKLIRSVEMYQRSESSSEECHDNYGGSQDCTTTYDYKKKWSDSAIDSSRFHETQGHQNPATREYESETREKEPIIVGAFTLDKAFTSQLTDDVKLPLNGQELKLPAQETEYTSSMNNNTETSAETSEMTEQLSGDASTGEQAN", "aa_hexdigest": "9b435ccec226f3e2f8664b1302f9a76a", "start_type": "GTG", "rbs_motif": "TTTTT", "genes": [], "truncated": "both", "pseudogene": { "start": 635612, "stop": 636646, "observations": { "mutation": [ 635881, 635917, 636571 ], "stop": [ 635881, 635917, 636571 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EH02", "query_cov": 1.0, "subject_cov": 0.47544642857142855, "identity": 0.995, "score": 406.0, "evalue": 1.22e-139, "gene_start": 1, "gene_end": 213, "reference_start": 31, "reference_end": 243, "reference_sequence": "MADRVSVTTRTGYGERVGNSFKKIFGGLLFVIGSIVLLARNENHFFVTKVGLNEGEKLVQDADAQQLNPELDGKLVHIQGVTSSPASSLQDPRFGVKADDLKLIRSVEMYQRSESSSEECHDNYGGSQDCTTTYDYKKKWSDSAIDSSRFHETQGHQNPATREYESETREKEPIIVGAFTLDKAFTSQLTDDVKLPLNGQELKLPAQETEYTSSMNNNTETSAETSEMTEQLSGDASTGEQANGALTPESANTRSGASSKKRFHVDDSMIYVGLDSHSPAVGDMRIRFSVVKTGTVSLVGKQSGNEITLYKTSNGVDLALLANGKKSSTEMFADAQAENLGLTWILRFVGLVLMYMGFAMMLEFIPTLAKVLPPLAGLIGFGTGVIAFAATLVLGLGTIAIAWLVVRPVIGIGLLVVAIGGAIAFIRSRKKDKKPDPQTKDSAEVVEV" }, "paralog": false, "identity": 0.9912790697674418, "score": 668.0, "evalue": 3.27e-245, "description": "Internal stop codon at 635881, 635917, 636571. Nonsense mutation around 635881, 635917, 636571.", "psc": { "uniref90_id": "UniRef90_A0A328EH02", "product": "Transmembrane protein 43", "uniref50_id": "UniRef50_A0A328EH02", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EH02", "UniRef:UniRef50_A0A328EH02" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EH02", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EH02" ] } }, "id": "NKOCPLAKPK_1199", "locus": "GCA003260355_01163" }, { "type": "cds", "sequence": "contig_1", "start": 636773, "stop": 636958, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATCGATCATCTCTCAGCAGGCAATCGAAGATTCAAAGGAGGAACAAAAAGAGAAATCTGATTTTAAACTCTTTTATACTGTTCCTCAAAGGGAATACGAAAACGTTTTCCCTGCACTCCATCTTCGGCACGGACTCAACATGCAACTACTAAACTCACCTCAGCTCCTTGAGGGAGGTTGA", "aa": "MKSIISQQAIEDSKEEQKEKSDFKLFYTVPQREYENVFPALHLRHGLNMQLLNSPQLLEGG", "aa_hexdigest": "5b63c465a01360ef7e4e90a5be6c8da9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7104.983699999999, "isoelectric_point": 5.554499626159667 }, "id": "NKOCPLAKPK_1200", "locus": "GCA003260355_01164" }, { "type": "cds", "sequence": "contig_1", "start": 636912, "stop": 637565, "strand": "-", "frame": 2, "gene": null, "product": "Nitroreductase family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C9LFK9", "UniRef:UniRef90_A0A328EL01" ], "nt": "ATGACCTTAGAAAAAATGCTTCAGCATCGCCGTGCAGTTCGTAGCTACAGTAAAACCGAGCGTATCGACTCAGAAAAAGTAAAAGAATGCCTCCAGCTTGCCCAGCTTGCACCGACGAGTTCTAATCTCCAGCTCCGAGAAGCCTATCATATCACGACTCCCAAAGTGATCACTCAAATAGCAGAGGCATGCTTTTCCCAGAGATCGGCGACGACAGCACAAGAAATAGTCGTTTTCATCACTCGTCAAGACCTCTATAAGCAGAGAGCAAAAGCGGTCTTTGATTTCAATCTCGAGGAAATCCAAAACAATGCTCCCAAAGACAGGATTCCTGCCAGGACCAAAAAGCTCCAGGACTATTATCAGAAGTTAATCCCTTTCCTCTATAGTCGTTGTTTCGGGGTATTGGGATGTTTTAGAAAAGGAGTATCTCGAATAAAAGGTTTCTCCAAGCCAACACTTCGTGAGGTATACGAATCTGATATGCAAACGGTAGTTCATAAGAGCTGTGCACTGGTCGCTCAAACCTTTATGCTTGCGATGTCTGAGCAAAATTATGATACTTGCCCGCTAGAAGGATTTGATAGCCAGAGGATCAAAAAGATTCTCAACCTCCCTCAAGGAGCTGAGGTGAGTTTAGTAGTTGCATGTTGA", "aa": "MTLEKMLQHRRAVRSYSKTERIDSEKVKECLQLAQLAPTSSNLQLREAYHITTPKVITQIAEACFSQRSATTAQEIVVFITRQDLYKQRAKAVFDFNLEEIQNNAPKDRIPARTKKLQDYYQKLIPFLYSRCFGVLGCFRKGVSRIKGFSKPTLREVYESDMQTVVHKSCALVAQTFMLAMSEQNYDTCPLEGFDSQRIKKILNLPQGAEVSLVVAC", "aa_hexdigest": "9b32c3ccac7d50857ee5e13a0fd3613c", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A328EL01", "query_cov": 1.0, "subject_cov": 0.9004149377593361, "identity": 0.982, "score": 416.0, "evalue": 1.72e-146, "valid": true, "product": "Nitroreductase family protein", "uniref50_id": "UniRef50_C9LFK9" }, "pscc": { "uniref50_id": "UniRef50_C9LFK9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C9LFK9" ], "product": "Nitroreductase family protein" }, "genes": [], "id": "NKOCPLAKPK_1201", "locus": "GCA003260355_01165" }, { "type": "cds", "sequence": "contig_1", "start": 637693, "stop": 638103, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGCAATTCTTGAGAATTTTATCGCCAAGGATCAGCCAATCATCTCCATTCTCTCCTCCGAGGGCAAGGAAGTCTATCCCTCAAAAGTTGCTTCCACAGCTCCTACGGTTCCGATGGTGATGATCATGGATGAGAAAACAGCGAGTGCAGCAGAGATCTTCGCTGGCGTGCTCAAACATTACTATCCAGGCAAGGTGAGCCTCGTAGGCACGACCAGCTATGGCAAGGGATCCGTCCAAGAGGTCGTGCAGTTCCAAGATAATTCCGTCCTCAAATATACAGTAGCACTACGATATGTCGCTGATCAGGAAGATTCCCTCAATGGAAAAGGTCTAAAACCAGACCTTATTCTCCGTGATGACCCCAAAACGCCACAGGATGAAGTGCTTACTGCGTTGGGAATCGACTAG", "aa": "MAILENFIAKDQPIISILSSEGKEVYPSKVASTAPTVPMVMIMDEKTASAAEIFAGVLKHYYPGKVSLVGTTSYGKGSVQEVVQFQDNSVLKYTVALRYVADQEDSLNGKGLKPDLILRDDPKTPQDEVLTALGID", "aa_hexdigest": "0e56ad5a00070c49bda166787f63be1f", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14720.700199999988, "isoelectric_point": 4.836623191833495 }, "id": "NKOCPLAKPK_1202", "locus": "GCA003260355_01166" }, { "type": "cds", "sequence": "contig_1", "start": 638203, "stop": 639168, "strand": "-", "frame": 1, "gene": null, "product": "PDZ domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EHC4", "UniRef:UniRef90_A0A328EHC4" ], "nt": "ATGAAAAAAATACTGTTCTTCCTACTTTTTGTCTGCTGTTGCTCAGCTGGAGTCAGTCTTGCCCAGGAGCAGGGGATGAGCTATGGAGAACTGCTCCGTCTTTACTTTACTGTCGTTGGACAGCAAGTTCCAAGCTCCTCACAGAAACCGCTTCTCAAATACAGCAACCTCACGCCAGCTTCTCCACTCTACCCGCTCCTACAAAATGCCGTAGCCAACGGTGCCTTCCCAAACCTTACGACCGCACTCCCTCTAGAGAAACCGGCTCTGGAGTCAGATTTAGCACTGCTCCTCAAGTATAATTTCAAAAAGCAGATCGTTTTCCAAGCAGGGAAGCCTTTGACTTTTGATGTGCTCAAGCAGCAGCTGCAGGAGGCTCTCCAAACACCTGCTCTTCCCAAAGAGGAAAAAGCTGAACCTACACCTACGATCTCCGATCAACAAAAGCAAACCATCGCCAAAGAGGTCCTCTCCCTCCTCAGAAAGAATTTCTCTCAAAAGGAGAAGATTGCCGAGCATGATGCTCCGAGCTATGATGAGCTATCTACGTATGTAGACAAGCTTGGAGAGCCGTATACCAAGTATTACACCCCGAGAAATGGGAAACTCTTTACTGACGCTTTGCAGGGAGGATTCGTGGGGATCGGGCTTTACCTAGAGCAAAAGGGAAGAGAACTGCCAGTTGTCGTGAAGCTCATAGAGAACAGCCCCGCCCAAAAGGCTGGAATCAAAGTTGGAGATATCCTCCTCGCAGCAGACGGGAAACCTTATGAATCCTATCATGATTTCGATGCCTTCATACTCGCATTGCAGGGAAAAGTTGGTACCTCTGTCAGCATCATGGTAAAAAGAGACCAGAGCCAGCTTACTTACGATCTCACGAGAGAGAAGATCGAGCTCCCTTTACTCAAGTCCCGCAAAAAAGGAAACGCCTGCTACCTTCAAATCTCCAGCTTTGACAAGTGA", "aa": "MKKILFFLLFVCCCSAGVSLAQEQGMSYGELLRLYFTVVGQQVPSSSQKPLLKYSNLTPASPLYPLLQNAVANGAFPNLTTALPLEKPALESDLALLLKYNFKKQIVFQAGKPLTFDVLKQQLQEALQTPALPKEEKAEPTPTISDQQKQTIAKEVLSLLRKNFSQKEKIAEHDAPSYDELSTYVDKLGEPYTKYYTPRNGKLFTDALQGGFVGIGLYLEQKGRELPVVVKLIENSPAQKAGIKVGDILLAADGKPYESYHDFDAFILALQGKVGTSVSIMVKRDQSQLTYDLTREKIELPLLKSRKKGNACYLQISSFDK", "aa_hexdigest": "c77bb16cc85f33bf1fe5a7998415f44a", "start_type": "ATG", "rbs_motif": "TTCTT", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 637900, "stop": 639222, "observations": { "mutation": [ 638124, 638166, 638205 ], "stop": [ 638124, 638166, 638205 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EHC4", "query_cov": 1.0, "subject_cov": 0.630648330058939, "identity": 0.975, "score": 607.0, "evalue": 3.61e-216, "gene_start": 1, "gene_end": 321, "reference_start": 19, "reference_end": 339, "reference_sequence": "MIPILTHRSCFILFSPQLMKKILFFLLFVCCCSVGVSLAQEQGMSYGELLRLYFTVVGQQVPSSSQKPLLKYSNLTPASPLYPLLQNAVANGAFPNLTTALPLEKPALESDLALLLKYNFKKEIVFQAGKPLTFDVLKQQLQEALQTPALSKEEKAEPTPTISDQQKQTIAKEVLSLLRKNFSQKEKIAEHDAPSYDELSTYVDKLGEPYTKYYTPRNGKLFTDALQGGFVGIGLYLEQKGRELPVVVKLVEKSPAQKAGIKVGDVLLAADGKPYEAYHDFDAFILALQGKVGTSVSIMVKRDQSQLTYDLTREKIELPLLKSRKKGSACYLQISSFDKGIQKKFLREKEEFGSCSVLYFDLRGNPGGIVDEVVAILENFIAKDQPIISILSSEGKEVYPSKVASTAPRAPMVMIMDEKTASAAEIFAGVLKHYYPDKVSLVGTTSYGKGSVQEVVQFQDNSVLKYTVALRYVADQEDSLNGKGLKPDLILRDDPKTPQDEVLTALGID" }, "paralog": false, "identity": 0.9681818181818181, "score": 834.0, "evalue": 5.37e-308, "description": "Internal stop codon at 638124, 638166, 638205. Nonsense mutation around 638124, 638166, 638205.", "psc": { "uniref90_id": "UniRef90_A0A328EHC4", "product": "PDZ domain-containing protein", "uniref50_id": "UniRef50_A0A328EHC4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EHC4", "UniRef:UniRef50_A0A328EHC4" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EHC4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EHC4" ], "product": "PDZ domain-containing protein" } }, "id": "NKOCPLAKPK_1203", "locus": "GCA003260355_01167" }, { "type": "cds", "sequence": "contig_1", "start": 639421, "stop": 640161, "strand": "+", "frame": 1, "gene": null, "product": "DNA alkylation repair protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C9RS79", "UniRef:UniRef90_A0A328EHN0" ], "nt": "TTGGGGAAGCTTTTAGATGAAAAAGAGAGATTTGCTACCATGAAGAAAGGGAAAATCTATGAGGAACTGCTGGTGCTAGCAGACGCGAAGAACGCAGGTTTTGTGCAACGCTTGATCCCGAACCTCGCACCAGAACGCATCCTGGGAACTAAGACGCCAATTCTCAGAACGTTCGCTAAGCAGCTCGCCAAAAGACCGGAACGTGAAGCCTTCCTCAAAGAGCTCCCCCATTTCTCCTTTGAAGAGCATCTGGTGCATGGGTTTGTGATTGAGGAGCTCAAGGATTTTGAGCAGACGATGGAAGCCCTCGAACACTACCTTCCCTTTATCGATAACCGAGCAACCTGTGATCAGCTCCGTCCCAAAATCCTTGCCAAGCATGCGGACGAAGTGCTGGTAAAGATTAGAGGGTGGCTAGGAAGTGAGCATCCCTATACGGTTAGGTATGCTATTGGACTCTTGATGACTAACTATTTGGATGAGGAGTTTGAGCCTGGACTGCTCCAGCTCGTGGCAGAGGTAGACCGTGAACACTACTATATTCAGATGATGCAGGCGTGGTATTTCGCTACTGCACTTGCAAAACAGCCCGCATCTACGCTTCCCCTCATCGAGAGTCAGACTTTAATACCTTTTGTCCAGAATAAAACGATCCAGAAAGCTAGAGAAAGTAGGAGAATCTCCCCTGAACTCAAAAAAGCCCTCCTCGCATGGAAAATAACCGAGAAGGGTAAGGGCTAG", "aa": "MGKLLDEKERFATMKKGKIYEELLVLADAKNAGFVQRLIPNLAPERILGTKTPILRTFAKQLAKRPEREAFLKELPHFSFEEHLVHGFVIEELKDFEQTMEALEHYLPFIDNRATCDQLRPKILAKHADEVLVKIRGWLGSEHPYTVRYAIGLLMTNYLDEEFEPGLLQLVAEVDREHYYIQMMQAWYFATALAKQPASTLPLIESQTLIPFVQNKTIQKARESRRISPELKKALLAWKITEKGKG", "aa_hexdigest": "b6873783fc609bbbb3f5f0141b627ef3", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A328EHN0", "query_cov": 0.9471544715447154, "subject_cov": 1.0, "identity": 1.0, "score": 460.0, "evalue": 1.74e-163, "valid": true, "product": "DNA alkylation repair protein", "uniref50_id": "UniRef50_C9RS79" }, "pscc": { "uniref50_id": "UniRef50_C9RS79", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C9RS79" ], "product": "3-methyladenine DNA glycosylase AlkD" }, "genes": [], "id": "NKOCPLAKPK_1204", "locus": "GCA003260355_01168" }, { "type": "cds", "sequence": "contig_1", "start": 640192, "stop": 640698, "strand": "-", "frame": 1, "gene": null, "product": "AAA+ ATPase domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EKG3", "UniRef:UniRef90_A0A328EKG3" ], "nt": "ATGAAGTTTGCCCTCAGGCAGAGACCTGATGTGATCCTCTTCGGAGAAATTAGAGATTTGGAAGGGATCAGAAATGCAATCCTCCTTTCAGAGACCGGGCATCTGGTGCTTACGACAGTGCACTCAAGGTCGGCAGAGCAGGCACTCAATAAGATCATCGGATCCTTTACCTCCGACGAACAAAATCATATTAGGACCCAGCTTGCAGAAAATATGTCAGCGATTGTCGTGCAGAAGCTCCTCAAGAGGACAGACAAAACAGGGCTCGTGCTTGGACAGGAAGTCTTGCTCAATACCACCGCCGTTGCCAATATTATTAGAGAAAATAAGCTGAACCAGCTCAGAAGTGTGATGTATACCAATAGACTTTCAGGGATGCAACTGATGGAGGAGAGCTTGATGATCCTGCTCGAAAAAGGCATGATCAGCATTGACCAAGCCATCGAAGCCGCCAACAATCCAGAATATATCAAGAGAGAATTGATCAATAGGGGATTGATCGGCTAA", "aa": "MKFALRQRPDVILFGEIRDLEGIRNAILLSETGHLVLTTVHSRSAEQALNKIIGSFTSDEQNHIRTQLAENMSAIVVQKLLKRTDKTGLVLGQEVLLNTTAVANIIRENKLNQLRSVMYTNRLSGMQLMEESLMILLEKGMISIDQAIEAANNPEYIKRELINRGLIG", "aa_hexdigest": "09f24b733a757bcc59469aa3f28b0d9e", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 640192, "stop": 640998, "observations": { "mutation": [ 640728 ], "stop": [ 640728 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EKG3", "query_cov": 1.0, "subject_cov": 0.4628099173553719, "identity": 1.0, "score": 317.0, "evalue": 2.84e-106, "gene_start": 1, "gene_end": 168, "reference_start": 196, "reference_end": 363, "reference_sequence": "MKFTDPVKYIEFLNDLMLQKNASDMYLTYDEPPTFRIMEEVYRDTTLEKLGDEQLNQIALTLMNKTGLDYFEKHGSLDLGLGYKDRRYRVNISRQRGHIMVVSRLLRKEIPDLKSLNLPPIFRELAYRENGIVFLAGPTGSGKSTTLAAMIEEINRNKKKHILTIEDPIEYVFTPKESIIDQKELGKDISSFSSAMKFALRQRPDVILFGEIRDLEGIRNAILLSETGHLVLTTVHSRSAEQALNKIIGSFTSDEQNHIRTQLAENMSAIVVQKLLKRTDKTGLVLGQEVLLNTTAVANIIRENKLNQLRSVMYTNRLSGMQLMEESLMILLEKGMISIDQAIEAANNPEYIKRELINRGLIG" }, "paralog": false, "identity": 0.996268656716418, "score": 507.0, "evalue": 1.03e-183, "description": "Internal stop codon at 640728. Nonsense mutation around 640728.", "psc": { "uniref90_id": "UniRef90_A0A328EKG3", "product": "AAA+ ATPase domain-containing protein", "uniref50_id": "UniRef50_A0A328EKG3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EKG3", "UniRef:UniRef50_A0A328EKG3" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EKG3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EKG3" ], "product": "AAA+ ATPase domain-containing protein" } }, "id": "NKOCPLAKPK_1205", "locus": "GCA003260355_01169" }, { "type": "cds", "sequence": "contig_1", "start": 640726, "stop": 641283, "strand": "-", "frame": 1, "gene": null, "product": "AAA+ ATPase domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EKG3", "UniRef:UniRef90_A0A328EKG3" ], "nt": "ATGAAGTTTACTGATCCGGTAAAGTATATTGAGTTTCTCAATGACCTGATGCTCCAGAAGAACGCTTCTGATATGTATCTTACCTATGATGAGCCACCGACTTTCAGGATTATGGAAGAGGTGTATAGAGATACGACCCTTGAGAAACTCGGCGACGAACAGCTCAACCAGATCGCGCTAACCTTGATGAACAAGACCGGACTGGATTATTTTGAGAAACATGGAAGTTTGGACCTTGGACTAGGCTACAAAGACAGAAGATACAGGGTGAACATCTCTCGTCAAAGGGGGCACATTATGGTGGTTTCTAGATTGCTGAGGAAGGAGATTCCTGACCTTAAAAGTTTAAACCTTCCGCCCATCTTCAGAGAGCTTGCCTATAGAGAAAATGGGATTGTGTTCCTTGCGGGACCTACCGGATCAGGAAAATCTACCACCTTGGCAGCGATGATAGAGGAGATCAATAGGAATAAAAAGAAGCATATCCTCACGATTGAAGACCCGATTGAATATGTTTTCACACCCAAAGAGAGTATTATTGATCAGAAAGAACTCTGA", "aa": "MKFTDPVKYIEFLNDLMLQKNASDMYLTYDEPPTFRIMEEVYRDTTLEKLGDEQLNQIALTLMNKTGLDYFEKHGSLDLGLGYKDRRYRVNISRQRGHIMVVSRLLRKEIPDLKSLNLPPIFRELAYRENGIVFLAGPTGSGKSTTLAAMIEEINRNKKKHILTIEDPIEYVFTPKESIIDQKEL", "aa_hexdigest": "286890af20b7d4fce1478c4d12954586", "start_type": "ATG", "rbs_motif": "TTTTT", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 640423, "stop": 641283, "observations": { "mutation": [ 640728 ], "stop": [ 640728 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EKG3", "query_cov": 1.0, "subject_cov": 0.509641873278237, "identity": 1.0, "score": 366.0, "evalue": 1.44e-125, "gene_start": 1, "gene_end": 185, "reference_start": 1, "reference_end": 185, "reference_sequence": "MKFTDPVKYIEFLNDLMLQKNASDMYLTYDEPPTFRIMEEVYRDTTLEKLGDEQLNQIALTLMNKTGLDYFEKHGSLDLGLGYKDRRYRVNISRQRGHIMVVSRLLRKEIPDLKSLNLPPIFRELAYRENGIVFLAGPTGSGKSTTLAAMIEEINRNKKKHILTIEDPIEYVFTPKESIIDQKELGKDISSFSSAMKFALRQRPDVILFGEIRDLEGIRNAILLSETGHLVLTTVHSRSAEQALNKIIGSFTSDEQNHIRTQLAENMSAIVVQKLLKRTDKTGLVLGQEVLLNTTAVANIIRENKLNQLRSVMYTNRLSGMQLMEESLMILLEKGMISIDQAIEAANNPEYIKRELINRGLIG" }, "paralog": false, "identity": 0.9965034965034965, "score": 555.0, "evalue": 3.85e-202, "description": "Internal stop codon at 640728. Nonsense mutation around 640728.", "psc": { "uniref90_id": "UniRef90_A0A328EKG3", "product": "AAA+ ATPase domain-containing protein", "uniref50_id": "UniRef50_A0A328EKG3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EKG3", "UniRef:UniRef50_A0A328EKG3" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EKG3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EKG3" ], "product": "AAA+ ATPase domain-containing protein" } }, "id": "NKOCPLAKPK_1206", "locus": "GCA003260355_01170" }, { "type": "cds", "sequence": "contig_1", "start": 641497, "stop": 641802, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCATTATAGGTTTAAAAAACCTATAGTCGCCACTACGAAACCAAAAATCAACTCAATTCATCCTACTCATAACGATGATGATGCAGAATTTAGGGGTCCTTTGGATAGTCAAGCTGTAAGCATAGGAGTTGATACCCTCTCCAATCATCAAGGAAACTATGGAAATGTTGTGGATGTGAAGCAGGAAAGTTCAGAAGAAGGGAATTTTTACACCTCCTATTTGTATTTCGAAGACATCCCCCTTCAGTTCGTGCAGGGCAATCGGTTTTTGCTGGAGAAACAAAGCTTAGTAATGCTAGTATGA", "aa": "MHYRFKKPIVATTKPKINSIHPTHNDDDAEFRGPLDSQAVSIGVDTLSNHQGNYGNVVDVKQESSEEGNFYTSYLYFEDIPLQFVQGNRFLLEKQSLVMLV", "aa_hexdigest": "470b059bd3d8d9da7f74ea086006cae8", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11519.7366, "isoelectric_point": 5.417347145080566 }, "id": "NKOCPLAKPK_1207", "locus": "GCA003260355_01171" }, { "type": "cds", "sequence": "contig_1", "start": 641794, "stop": 642582, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCAAACTGTCAGCGAGGACCCTTTTTCACTGTTTCACGGTCTGCAAACTCCTTGCGAGGCTCCTTTTCCACCTTTTCACGATCTGCAAACTGTCAGCGAGGACCCTTTTCCATCTTTTCACGCTGTGCAAACTGTCAGCGAGGCTCCTTTTTCACCTTTTCACGGTCTGCAAACTGTCAGCGAGGACCCTTTTCCACCGTTTCACGGTCTGCAAACTGTCAGCGAGGGGTCTTTTTTATCGTTGCACGCTGTGCAAACCCTGAGCAGAGGAGTCCGCTTCCTCCTTGCACCTCTTGCAAGGCTATTGCAGGAGGTTTTTCTCCCCATTCACCAACGCTACCTACCGCTGGAAACCAGCTTTTCCCTTTTGTTCTTTACCCCGACTATAGCAATCCCTCACCCTCTGTCAAGGGAAAATCACCCGAAAATCCAAAAAAAGCCCCCTTTTTGCTCTTCCCTCGCCATCCTCACTGCCAAAATCCCTCCATTCCCCCAGCTTGAACGCAAGGAGGGCTGGCAACCACGACGATGCCCCAAGGGGAAAAACAAGAAAGCAGACCTAGAAAAGGGCGAGTCCTATACTTCTCTCGGTGGGCAATCTGGTTTTATGCTGGTGGATTGGAAAGAAGTACCTCCAGCGACGATCAAAACGACTGCACGATCTCCAGCGCTTCCTTTGATAAGAAGGGATCAAAGGAAAATACTTTTCCCTCCTAGCATGCTCCATTATTCAAGTAAAGGGCATCGTGAGCATGAGGACTCCTTGATGTTCCCCCGCTCATACTAG", "aa": "MQTVSEDPFSLFHGLQTPCEAPFPPFHDLQTVSEDPFPSFHAVQTVSEAPFSPFHGLQTVSEDPFPPFHGLQTVSEGSFLSLHAVQTLSRGVRFLLAPLARLLQEVFLPIHQRYLPLETSFSLLFFTPTIAIPHPLSRENHPKIQKKPPFCSSLAILTAKIPPFPQLERKEGWQPRRCPKGKNKKADLEKGESYTSLGGQSGFMLVDWKEVPPATIKTTARSPALPLIRRDQRKILFPPSMLHYSSKGHREHEDSLMFPRSY", "aa_hexdigest": "4bda34200b30a16740936eae53c23d71", "start_type": "GTG", "rbs_motif": "TTTTTA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 29613.71950000003, "isoelectric_point": 8.975111198425292 }, "id": "NKOCPLAKPK_1208", "locus": "GCA003260355_01172" }, { "type": "cds", "sequence": "contig_1", "start": 642981, "stop": 643292, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAAAGGAAACTTTACAGGTCTTCGGAGTGAAGAGCCTGGCGAATGCGAGCTATGGGGCGTTTATGGAAGCGGTGAAAAGTCTCATCGCGAAAGGGACGACCGAGAAGCTCGGGCTGAAAGCGGAAGATTTTTCTGCTTTTGAAGCGTTGCTTGTGCAGCTTCTTGAGGTCAACAACAGCAGCAAGAAGGATGCCACGACCAAGGAACTTGATGCGTTGGATGAGCAACGTGATCGCCTGTTGACGCATCTGTTTGCGAGGGTGGCGCTCGAGGCGGGGTCGCCTGATGAAACTAGTCAAAAAGCCTGA", "aa": "MEKETLQVFGVKSLANASYGAFMEAVKSLIAKGTTEKLGLKAEDFSAFEALLVQLLEVNNSSKKDATTKELDALDEQRDRLLTHLFARVALEAGSPDETSQKA", "aa_hexdigest": "5f8a96a12ca18af0058ceffe6e157704", "start_type": "ATG", "rbs_motif": "TTCTA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11219.587499999983, "isoelectric_point": 4.99213466644287 }, "id": "NKOCPLAKPK_1209", "locus": "GCA003260355_01173" }, { "type": "cds", "sequence": "contig_1", "start": 643302, "stop": 643769, "strand": "+", "frame": 3, "gene": null, "product": "DUF6261 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EGP2", "UniRef:UniRef90_A0A328EGP2" ], "nt": "ATGGGGCTTAGAGAACTCTACAAAGGGATTCAAAGAAAGCCGACTCGCGAAGAGACCTATTTGATTAAAGGGCTGTTGGTAGACGCTGGCAAAGCGGAGCATAACGCGGCGTTTGTGAAGCTCGGGCTTAAGCCGATGCTCGACCAGCTCAAAGCGATCAATGAAGACTATGAGAAGAAGACTGCCTCCCGTGCAGCATCTCAAGTAGCAAACACCTTGACTCCGGCGAAGGAGCTTCGCAAGCAATTGGATCAGTATTATGAAGCGTTTATGATGAAGATTGGGGCGTTGGCGATTGTCAGCCCGAATCCAGAACTAGAAGCGTTTGTAAAGACGTTTAATAAGCTGGCGAAGGACACGAGAGAACGCTGGAAGTTGCACCTTGCTCAGCTCGGGCTTTGGACCGAAGAAGGTGGGCGAGAGGACGAGGATGGCAATGCAGAAAGTGAAACCGAAGCAGAGAAATAA", "aa": "MGLRELYKGIQRKPTREETYLIKGLLVDAGKAEHNAAFVKLGLKPMLDQLKAINEDYEKKTASRAASQVANTLTPAKELRKQLDQYYEAFMMKIGALAIVSPNPELEAFVKTFNKLAKDTRERWKLHLAQLGLWTEEGGREDEDGNAESETEAEK", "aa_hexdigest": "3f218875f73088fc810393deee6710df", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 643002, "stop": 643769, "observations": { "mutation": [ 643290 ], "stop": [ 643290 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EGP2", "query_cov": 1.0, "subject_cov": 0.5916030534351145, "identity": 1.0, "score": 301.0, "evalue": 7.07e-102, "gene_start": 1, "gene_end": 155, "reference_start": 108, "reference_end": 262, "reference_sequence": "MEKETLQVFGVKSLANASYGAFMEAVKSLIAKGTTEKLGLKAEDFSAFEALLVQLLEVNNSSKKDATTKELDALDEQRDRLLTHLFARVALEAGSPDETSQKAGLQLMGLRELYKGIQRKPTREETYLIKGLLVDAGKAEHNAAFVKLGLKPMLDQLKAINEDYEKKTASRAASQVANTLTPAKELRKQLDQYYEAFMMKIGALAIVSPNPELEAFVKTFNKLAKDTRERWKLHLAQLGLWTEEGGREDEDGNAESETEAEK" }, "paralog": false, "identity": 0.996078431372549, "score": 490.0, "evalue": 1.13e-178, "description": "Internal stop codon at 643290. Nonsense mutation around 643290.", "psc": { "uniref90_id": "UniRef90_A0A328EGP2", "product": "Family of unknown function (DUF6261)", "uniref50_id": "UniRef50_A0A328EGP2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EGP2", "UniRef:UniRef50_A0A328EGP2" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EGP2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EGP2" ] } }, "id": "NKOCPLAKPK_1210", "locus": "GCA003260355_01174" }, { "type": "cds", "sequence": "contig_1", "start": 643861, "stop": 644277, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EIW2", "UniRef:UniRef90_A0A328EIW2" ], "nt": "ATGGGAAATGAAGATTTTGGGTTAGCAAAAGAGTTCCTTGCTGGAGCAGGGGTTCGAGAGTTCCTTCCTGAGGGATTGCCCTGTGATGAAAGATACAAAAGAGGCGATAATCGGTGGAGAAACTCAAGTAAAGAGGAGCAAGCACAGGTGTTGCAACTCTTACAAACAGTGCTTTCATGTCCACTTACTGAGGATTGTGTGACGTATCGTCGTGCTCCAAGTCCCCTTGGAGTGGAGTGCTTGGTATTAGATGAGCAAGAACAGAAGCTTTATACCTATTGGCTGGAGGGCGGCATGGAAGCTCCTTATGGAGATGATCTAATTATTTACCTAGCTTCTTATACTGCAAGGAGACGGGACAAGAATGCGATCTTTTTTAGTCATCACGATCTCTCTCAATCTCAGGCTTCTTGCTGA", "aa": "MGNEDFGLAKEFLAGAGVREFLPEGLPCDERYKRGDNRWRNSSKEEQAQVLQLLQTVLSCPLTEDCVTYRRAPSPLGVECLVLDEQEQKLYTYWLEGGMEAPYGDDLIIYLASYTARRRDKNAIFFSHHDLSQSQASC", "aa_hexdigest": "734d55ac93026add25667e62c011cfaa", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A328EIW2", "query_cov": 1.0, "subject_cov": 0.8518518518518519, "identity": 1.0, "score": 286.0, "evalue": 7.05e-98, "valid": true, "uniref50_id": "UniRef50_A0A328EIW2" }, "pscc": { "uniref50_id": "UniRef50_A0A328EIW2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EIW2" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15730.46909999999, "isoelectric_point": 4.831791877746581 }, "id": "NKOCPLAKPK_1211", "locus": "GCA003260355_01175" }, { "type": "cds", "sequence": "contig_1", "start": 644459, "stop": 644875, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EIW2" ], "nt": "ATGGGAAATGAAGATTTTGGGTTAGCAAAAAAGTTCCTTGCTGGAGCAGGGGTTCGAGAGTTCCTTCCTGAGGGGTCCCACTGTGATGAAAAATACAAAAGAGGCGATAATCGGTGGAGAAACTCAAGTAAAGAGGAGCAAGCACAGGTGTTGCAACGCTTGCAAATAGTGCTTTCTCGTCCACTTACTGAGGACTGTAAGAAGTATCTTCCTGCTTCAAGTCCCCTTGGAATGAAGTTGATCGTATTAGATGAGCAAGAACAGAAGCTTTTTACCTATTTGCTGCAGGGCGGCAGGAGAGGTCCTTATGGAGATGATCTAGTTATTTACCTGGGTGCTTATACTGACAGGAGACCAGACAAGGAGGAGATCCTTTTGAGTCATTGCGATCTCTCTCATCCACAGGCTTCTTGCTGA", "aa": "MGNEDFGLAKKFLAGAGVREFLPEGSHCDEKYKRGDNRWRNSSKEEQAQVLQRLQIVLSRPLTEDCKKYLPASSPLGMKLIVLDEQEQKLFTYLLQGGRRGPYGDDLVIYLGAYTDRRPDKEEILLSHCDLSHPQASC", "aa_hexdigest": "6bb850636eebef3445be4ed42b03d715", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A328EIW2", "query_cov": 1.0, "subject_cov": 0.8518518518518519, "identity": 0.754, "score": 208.0, "evalue": 6.11e-67, "valid": false, "uniref50_id": "UniRef50_A0A328EIW2" }, "pscc": { "uniref50_id": "UniRef50_A0A328EIW2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EIW2" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15687.644999999991, "isoelectric_point": 6.301932334899903 }, "id": "NKOCPLAKPK_1212", "locus": "GCA003260355_01176" }, { "type": "cds", "sequence": "contig_1", "start": 644900, "stop": 645112, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGGGGGACGGGTTTGTATTGTTTTTATTATTGTGCCACTACTATAATCATTTATTACCCAAATGTCAAGGGTTTGTGAACTCAATGAAAAAAGTCTGGCTCCGAGATACCGGGGTCAGACTTTTGGGATTGAGAGAGGAGCATCTAGCTTCTCAATGGGTGGAGCTGTCTCTGAAGGATGCCGCCCACCGTATCAAGAAACTTACCTGA", "aa": "MKGDGFVLFLLLCHYYNHLLPKCQGFVNSMKKVWLRDTGVRLLGLREEHLASQWVELSLKDAAHRIKKLT", "aa_hexdigest": "6a9306a6d4d22c861c778d3d86acd44e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8166.618699999993, "isoelectric_point": 9.564932441711427 }, "id": "NKOCPLAKPK_1213", "locus": "GCA003260355_01177" }, { "type": "cds", "sequence": "contig_1", "start": 645047, "stop": 645469, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGAAAAAAAATTAAACAGACCAAACCGTGCTCAGAGAAGCTTGATATTGACGAACTTAGTAATATTCCCTATCCTACCCTCAGACTTTACCCCAAAGCAAGTCGCCAATGGCTAGTGCTGAGTAAAGAGGAGCAAATAGCACTCATTGACGCACGGGAAGAAATGTTGAATCATCCATTTTATGAGGACTATTCAGAACATGTCATCTGTGTTGGAGATTTTTTCAAGGAGCCGATACCTTTAAATCACAAGGAAAATAAAATTCTAACCCTTATCTTTGAAAACTGTGTTCGGCTTTGCAACCGTGCGATAGCACGGATCTGCCATGATATTGAGAGTGATATCAGGCGAGGATCAGGTAAGTTTCTTGATACGGTGGGCGGCATCCTTCAGAGACAGCTCCACCCATTGAGAAGCTAG", "aa": "MGKKIKQTKPCSEKLDIDELSNIPYPTLRLYPKASRQWLVLSKEEQIALIDAREEMLNHPFYEDYSEHVICVGDFFKEPIPLNHKENKILTLIFENCVRLCNRAIARICHDIESDIRRGSGKFLDTVGGILQRQLHPLRS", "aa_hexdigest": "81eb3a37958ea0bc4e313d48671458a7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16289.706999999984, "isoelectric_point": 7.663226127624512 }, "id": "NKOCPLAKPK_1214", "locus": "GCA003260355_01178" }, { "type": "cds", "sequence": "contig_1", "start": 645471, "stop": 645578, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATGTATTACTATGTCCCCTCTGGGTGGAAAGGACAGGTTACTCTTATTTTTATTATCACGGGACTCACTATATGTATTCGTTCCGTAAATGTCAAGGGTTTGTAA", "aa": "MMYYYVPSGWKGQVTLIFIITGLTICIRSVNVKGL", "aa_hexdigest": "9d1a5336e23e10397c8553da3abd2e51", "start_type": "ATG", "rbs_motif": "TTTA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3965.7871000000005, "isoelectric_point": 9.515291786193849 }, "id": "NKOCPLAKPK_1215", "locus": "GCA003260355_01179" }, { "type": "cds", "sequence": "contig_1", "start": 645653, "stop": 646069, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EKJ6", "UniRef:UniRef90_A0A328EKJ6" ], "nt": "ATGAATGAGAAAAATTGTAAGCTAGCAATGGAGTTTTTGAGTGGAGCTGGCGTTCTTATGTTTATGGACGCAGACTGTTCTCATGGTATTGAGTTTGGGTATGCAGATAGCCTATGGAAGATGTCTAGCAAAAAGGAGCGAGCAGAGCTTTTATTTTGTCTGCAATCAATCCTCACGCTCCCTGCTACTGAGGAATATATAAAGTATACTCCTCTTTTCCGCAGACTCAACGAGGAAACGGTCGTGCTAAACGAACAAGAAAGGAAAGTTTGGACAGCTATTCGTGATCACCATACTGAGAATCCTTATAGAGAGGAGTTGGAAATCTATCTTGATGCCCACGTCGAGATCAGGATGGCATACCTTGCTCATCTTGACGACCTAGCCGCAACTCTTCGGAAGACCTGTTCTTGCTGA", "aa": "MNEKNCKLAMEFLSGAGVLMFMDADCSHGIEFGYADSLWKMSSKKERAELLFCLQSILTLPATEEYIKYTPLFRRLNEETVVLNEQERKVWTAIRDHHTENPYREELEIYLDAHVEIRMAYLAHLDDLAATLRKTCSC", "aa_hexdigest": "4a2ca369f62fbdcddc98a9208d40f4a3", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 645482, "stop": 646069, "observations": { "mutation": [ 645583, 645616, 645655 ], "stop": [ 645583, 645616, 645655 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EKJ6", "query_cov": 1.0, "subject_cov": 0.7076923076923077, "identity": 1.0, "score": 281.0, "evalue": 4.23e-95, "gene_start": 1, "gene_end": 138, "reference_start": 1, "reference_end": 138, "reference_sequence": "MNEKNCKLAMEFLSGAGVLMFMDADCSHGIEFGYADSLWKMSSKKERAELLFCLQSILTLPATEEYIKYTPLFRRLNEETVVLNEQERKVWTAIRDHHTENPYREELEIYLDAHVEIRMAYLAHLDDLAATLRKTCSCGDSLSLVSKIPQVGLWYLRSDFFHGVYKPLTFTERIHIVSPVIIKIRVTCPFHPEGT" }, "paralog": false, "identity": 0.9846153846153847, "score": 391.0, "evalue": 6.87e-141, "description": "Internal stop codon at 645583, 645616, 645655. Nonsense mutation around 645583, 645616, 645655.", "psc": { "uniref90_id": "UniRef90_A0A328EKJ6", "uniref50_id": "UniRef50_A0A328EKJ6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EKJ6", "UniRef:UniRef50_A0A328EKJ6" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EKJ6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EKJ6" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 16069.296799999995, "isoelectric_point": 5.224151420593261 }, "id": "NKOCPLAKPK_1216", "locus": "GCA003260355_01180" }, { "type": "cds", "sequence": "contig_1", "start": 646251, "stop": 646715, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBF76B2", "UniRef:UniRef100_A0A328EH12", "UniRef:UniRef50_A0A328EH12", "UniRef:UniRef90_A0A328EH12" ], "nt": "ATGAATATGGGAAATAAAGATTTAAGTTCAGTAGAGGGATTCCTTATTGGAGGAGTTCGTGATTTAAGTTTAGCAGAGCAGTTTCTCACTGGAGCAGGAGTCCGTAAGTTTACTCACCCTCGTTCTTTTACGCATGATCGCTACCACGGCGGTGATGCCTGCTGGAACAAGCTATCTCCCGCCCAGCAAGAAGGGGTTATTCAGATCCTACAACTTGCACTCTCGGAACCGCTTCCCGAGGAATGCATCGGGTGCTATGTCTTCTCCGACCATCCCGACCAACCGACACTCGTCTTGGATGACTCGCAAAGGCAGCTTATTACCTATCTGAGGGGAGTCGAGCTTGACAATTTTTTTGTCAATGTGCTGCTTGATCTGCTTGTTGCTCACTACACCATTAGATCGGGCAATATAGTAAGCCCTGCAAGGTTGAAACAATCCTTCAGGATGCTAATCGCAAAGTAA", "aa": "MNMGNKDLSSVEGFLIGGVRDLSLAEQFLTGAGVRKFTHPRSFTHDRYHGGDACWNKLSPAQQEGVIQILQLALSEPLPEECIGCYVFSDHPDQPTLVLDDSQRQLITYLRGVELDNFFVNVLLDLLVAHYTIRSGNIVSPARLKQSFRMLIAK", "aa_hexdigest": "d828e78fe5f8509cfa386f8ff8fcb010", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000DBF76B2", "uniref100_id": "UniRef100_A0A328EH12", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBF76B2", "UniRef:UniRef100_A0A328EH12" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EH12", "uniref90_id": "UniRef90_A0A328EH12" }, "psc": { "uniref90_id": "UniRef90_A0A328EH12", "uniref50_id": "UniRef50_A0A328EH12" }, "pscc": { "uniref50_id": "UniRef50_A0A328EH12", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EH12" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17231.535099999997, "isoelectric_point": 5.909402275085449 }, "id": "NKOCPLAKPK_1217", "locus": "GCA003260355_01181" }, { "type": "cds", "sequence": "contig_1", "start": 646892, "stop": 647308, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EL09", "UniRef:UniRef90_A0A328EL09" ], "nt": "ATGGGAAATAAAAACTGCAGATCTGCGAAACGCTTCCTGCGGGAAGCAGGAGTTCTTATGATTGGTGGTCAAGGGAGCGCGTTGTCCAATCCTTACCTGCTGGCAAACCATGCGTGGCTGAGATTGAGTGAAAAGGAGCAAGAGGCCCTTTTAGAAATGTTGGAATCAGTATTAGCCTTGCCATATTCTGGGAAGCCTATTGAGTATCTCCTTAGCTCTGGAGCCCTTGAAGGGATACGGGTAGTTTTAAATGAGGAAGAAAGTAAACTTCTGACCTTTATCTACGATCAACCTCTCAGAGCTTATGAGAGAGTCATCTTGCAAACCTATCTTGATGCTCACGTCGAGATCAGGAATCGCTACCCCGACTCTTTTGCCAAACAGAATAATGTCCGTCGAGAACCGGTCCTCTGCTGA", "aa": "MGNKNCRSAKRFLREAGVLMIGGQGSALSNPYLLANHAWLRLSEKEQEALLEMLESVLALPYSGKPIEYLLSSGALEGIRVVLNEEESKLLTFIYDQPLRAYERVILQTYLDAHVEIRNRYPDSFAKQNNVRREPVLC", "aa_hexdigest": "931746efc606b470b2f23456921e83d6", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A328EL09", "query_cov": 1.0, "subject_cov": 0.8466257668711656, "identity": 1.0, "score": 274.0, "evalue": 5.53e-93, "valid": true, "uniref50_id": "UniRef50_A0A328EL09" }, "pscc": { "uniref50_id": "UniRef50_A0A328EL09", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EL09" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15710.912999999988, "isoelectric_point": 6.741127204895021 }, "id": "NKOCPLAKPK_1218", "locus": "GCA003260355_01182" }, { "type": "cds", "sequence": "contig_1", "start": 647501, "stop": 647989, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBF6C35", "UniRef:UniRef100_A0A328EHD3", "UniRef:UniRef50_A0A328EHD3", "UniRef:UniRef90_A0A328EHD3" ], "nt": "ATGGGAAAGAAGAATAATGAATTACTAGAAGAAACGTTTGATAAAGCTTACCGTTTCTTATCGGGAGTAGGAGTTTCCTGCCCTGGTTGTAGAGATTGCAAAAGAGTCAAGGGGAGCCCCGGTGATAAATTATGGCAGGGTCTGGACCCTACGATGCGGACTAACATCATCGCAATGCTGGCGGATGCTCTCAATGGACCATCAATCAAGGAGTCTGTGGTCATAAAACCCTTCCTCAGTTATCTCAGAGTGGTGAAGATGGCACTGGATGATGAGGAAAAAAAGATACTAGCCTTCTTGGCAGAGCTTGAGATGCATACTCCTATCGTGGAGGCTATCCTTGATCTGCTTGTTGTCCATCACCTCGGTAAATTTGAGGGGAAGAATCGCCCCAGGCAGCTCACTCCGGTCAATCTGAAGCATTACTTCAAACAGTATCTTCGCCTGTTCCGTAAACAGATGCCTTTGACACGCATGGCTTCTCGATAA", "aa": "MGKKNNELLEETFDKAYRFLSGVGVSCPGCRDCKRVKGSPGDKLWQGLDPTMRTNIIAMLADALNGPSIKESVVIKPFLSYLRVVKMALDDEEKKILAFLAELEMHTPIVEAILDLLVVHHLGKFEGKNRPRQLTPVNLKHYFKQYLRLFRKQMPLTRMASR", "aa_hexdigest": "25ab6ed0f45c47783099036eeb16a4d1", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000DBF6C35", "uniref100_id": "UniRef100_A0A328EHD3", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBF6C35", "UniRef:UniRef100_A0A328EHD3" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EHD3", "uniref90_id": "UniRef90_A0A328EHD3" }, "psc": { "uniref90_id": "UniRef90_A0A328EHD3", "uniref50_id": "UniRef50_A0A328EHD3" }, "pscc": { "uniref50_id": "UniRef50_A0A328EHD3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EHD3" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18513.690400000003, "isoelectric_point": 9.587431907653805 }, "id": "NKOCPLAKPK_1219", "locus": "GCA003260355_01183" }, { "type": "cds", "sequence": "contig_1", "start": 648176, "stop": 648715, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EKH4", "UniRef:UniRef90_A0A328EKH4" ], "nt": "ATGGAAACTCAAAAATGTACCTTGGTAAATGAAATGTGTGATGTTGTGTATAAATTCCTGGAGGAAGCGGGGATCTCTAAGGTATTACTTAAAGGATGCTCGGGAGAGCCTGATAGTCGAGAGGAGAGAAGCTTGATAGGACTGCGTTTCTATTTTGGTAGTCGAGCTGAGAATATCTGGATGGGGCTCGGTTTCGAAAAGCAGAACATGGTCTACAATACATTGCGGACTGCTCTCAACAGGCCACTTGCCATGGGAGCTGAGGTATTCCATGTCCCCTTTGCATATTCCGAAAAGCTCGAGACAGCTCTGGATGAGTCGGAAAAAAAGGTTTTATTCTTCTTGAATATGACAAATATTGACTATTCTGATGAATATCCGATACTTCTGCTCCTTTGCGCACGTCACCACCATAAATATGGGGGGACGGAGCATGCCGAGCAACCGCTCGAAAACAGCGTGGAGCTCATCGCCAGTGCAGATAACAGGTTAAAGACTATGCGGGAGTCCTTTGAACAATCTACATTTCGTCGGGTTTGA", "aa": "METQKCTLVNEMCDVVYKFLEEAGISKVLLKGCSGEPDSREERSLIGLRFYFGSRAENIWMGLGFEKQNMVYNTLRTALNRPLAMGAEVFHVPFAYSEKLETALDESEKKVLFFLNMTNIDYSDEYPILLLLCARHHHKYGGTEHAEQPLENSVELIASADNRLKTMRESFEQSTFRRV", "aa_hexdigest": "1b6e41b2a294595f04bdd74630b1ff9b", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A328EKH4", "query_cov": 1.0, "subject_cov": 0.8364485981308412, "identity": 1.0, "score": 361.0, "evalue": 8.74e-126, "valid": true, "uniref50_id": "UniRef50_A0A328EKH4" }, "pscc": { "uniref50_id": "UniRef50_A0A328EKH4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EKH4" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 20615.313800000007, "isoelectric_point": 5.447414970397949 }, "id": "NKOCPLAKPK_1220", "locus": "GCA003260355_01184" }, { "type": "cds", "sequence": "contig_1", "start": 648896, "stop": 649315, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EH64", "UniRef:UniRef90_A0A328EH64" ], "nt": "ATGCAAAAAGAAAAGTTCGGGGCAGTAATGGAATTCTTAAGGGGAGCTGGCTTCGGGGAAATAGAGAAACCTGATGATCTTGCCCTTTATTGCACTCAAGCTGGCAATAAGCCGTGGTCGGAAATGGATGAAAGAGATCAAAGAACATTCCTAGATCTCTTGGAAGCTATTCTTTCTTCAGCTCTTCCCCAGACCTACCTAAGAGAACGCCTTATCTTTAAAGGCAACAAAAAGGTATTGACCGGCTTCGATCTCTCAGAACGTAAAGTTCTTGCCTTTTTGGAGAAGTCCACTATTAGAGAATCTCCCGTGGTAGAGGCTTTAATAGATTGTCTGGTTGCTCATAACTCGATCAGGTTGGGATATATGATCGCCCCCTCCCTATTAAAGGAAGCTATTAGAGATGGTCTCTCCCGTTGA", "aa": "MQKEKFGAVMEFLRGAGFGEIEKPDDLALYCTQAGNKPWSEMDERDQRTFLDLLEAILSSALPQTYLRERLIFKGNKKVLTGFDLSERKVLAFLEKSTIRESPVVEALIDCLVAHNSIRLGYMIAPSLLKEAIRDGLSR", "aa_hexdigest": "e1e064820b30e9a7540027239fd6bdf5", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A328EH64", "query_cov": 1.0, "subject_cov": 0.8081395348837209, "identity": 1.0, "score": 277.0, "evalue": 6.71e-94, "valid": true, "uniref50_id": "UniRef50_A0A328EH64" }, "pscc": { "uniref50_id": "UniRef50_A0A328EH64", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EH64" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15723.064899999988, "isoelectric_point": 5.915768241882325 }, "id": "NKOCPLAKPK_1221", "locus": "GCA003260355_01185" }, { "type": "cds", "sequence": "contig_1", "start": 649486, "stop": 649767, "strand": "-", "frame": 1, "gene": "sufD", "product": "SufD family Fe-S cluster assembly protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A948C5Q9" ], "nt": "GTGAACTGTGATGCTTTGATCCTTGATGAGGAGTCGGTGAGTAAGACGATTCCTGTGATTGAATGTGATAATGCTTCAGCTTTGGTTTCTCATGAGGCTTCTGCTGGAAAGGTAGATGAATTTCAGCTCTTCTACCTGATGAGTAGAGGGCTAGAAACCGAAAAGGCGATGTCTTTACTCGTGAATGGCTTCGTTTCCAGCGTGGTGAAACAGCTCCCTCTCGAATATGCAGGAGAACTCAATCGCTTGATTGAGCTCGAGATGGAGGGGAGTGTGGGCTAA", "aa": "MNCDALILDEESVSKTIPVIECDNASALVSHEASAGKVDEFQLFYLMSRGLETEKAMSLLVNGFVSSVVKQLPLEYAGELNRLIELEMEGSVG", "aa_hexdigest": "d3bab0f4e47cde1587595ba029cf1cf5", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A948C5Q9", "query_cov": 0.8064516129032258, "subject_cov": 0.8522727272727273, "identity": 0.68, "score": 110.0, "evalue": 9.85e-30, "valid": false, "product": "SufD family Fe-S cluster assembly protein", "uniref50_id": "UniRef50_A0A948C5Q9" }, "pscc": { "uniref50_id": "UniRef50_A0A948C5Q9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A948C5Q9" ], "product": "SufD family Fe-S cluster assembly protein" }, "genes": [ "sufD" ], "id": "NKOCPLAKPK_1222", "locus": "GCA003260355_01186" }, { "type": "cds", "sequence": "contig_1", "start": 649795, "stop": 650118, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01458.23" ], "nt": "ATGAGATATTCATCAGTTGAAAACTGGTCCACTAACACCTACAACCTCAATACCAAAAGAGCTCTGGTTCAGGATTTTGGTCTAATGGAGCGAATCGGTGGAAATCTCGGCTCTGGAGCTACGATGCTTTATCCTTGCTCAATCTTGCAGGGCGAGGGAGCCAAAGCAGACAATCTGTCGGTCGTTATGGCGTCCAAAGGACAGCATCAGGACGTCGGTTCCAAGGTGATCCACCTCGGCAAAAACACCAGCTCAACCATCATCTCCAAATCCCTTTCTCTCCAAGGCGGAATCAATACCTATAGAGGACTCGTAAAAATCTGA", "aa": "MRYSSVENWSTNTYNLNTKRALVQDFGLMERIGGNLGSGATMLYPCSILQGEGAKADNLSVVMASKGQHQDVGSKVIHLGKNTSSTIISKSLSLQGGINTYRGLVKI", "aa_hexdigest": "90f013e8ff66d7be8bfb99efab3f658e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01458.23", "name": "SUFBD_core", "length": 106, "aa_cov": 0.9906542056074766, "hmm_cov": 1.0, "evalue": 1.4660137130771656e-27, "score": 93.02609252929688, "start": 2, "stop": 107 } ], "seq_stats": { "molecular_weight": 11478.974799999989, "isoelectric_point": 9.609931373596194 }, "id": "NKOCPLAKPK_1223", "locus": "GCA003260355_01187" }, { "type": "cds", "sequence": "contig_1", "start": 650194, "stop": 650601, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCCAGATGAGATCAAAGCTACTTTCCAGAAGCTCGGAATCCCTGAAGCAGAGCAAAAATACCTTGCTGGAGCAGGAGGGCAGTTTGATTCTACAGCGGTCTATCACAAGATCAAGGAAGTCCGAGCAAAGCAGGGGATTATCTTTGAAGATATGAGTCATGCCCTCAAATCCTATCCTGAGCTGGTCCAAAAATACTTTATGAAGCTCGTTCCACCGACTGATCACAAATTCGCTGCCTTGCATGGTGCGGTTTGGAGTGGGGGAAGTTTTGTCTATATTCCCAAAGGGGTAAAGCTGACCCAGCCTTTGCAAGCCTATTTCCGTATGAATACCCTCGCTGGAGGGCAGTTTGAACATACGCTGATTATCGTGGAAGATGAGGCAGAAGCCCAGTATATTGAGTGA", "aa": "MPDEIKATFQKLGIPEAEQKYLAGAGGQFDSTAVYHKIKEVRAKQGIIFEDMSHALKSYPELVQKYFMKLVPPTDHKFAALHGAVWSGGSFVYIPKGVKLTQPLQAYFRMNTLAGGQFEHTLIIVEDEAEAQYIE", "aa_hexdigest": "f921d490635c6d2939e303c30c8bc748", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15135.217499999986, "isoelectric_point": 6.200474739074706 }, "id": "NKOCPLAKPK_1224", "locus": "GCA003260355_01188" }, { "type": "cds", "sequence": "contig_1", "start": 650995, "stop": 651390, "strand": "-", "frame": 1, "gene": null, "product": "Ribosomal RNA small subunit methyltransferase E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MSC0", "UniRef:UniRef90_A0A328EIX2" ], "nt": "ATGTTGCTTTCCTTGCCGAATAAACAGGAAAAACTCGAGCTGATCATCCAGAAGTTTACCGAAATAGGAGTTGATGAAATCTACCTCTGGGTTAGTGAGAGATCCGTCCTCAAAGCCATAAATCCCAATAAACTCCAAAGACTCTCCAAAATTATGCAAGAGGCAACAGAGCAATCTTGGAATCGATCTTTGCCTAAACTAAGCTTTATTGAAGCTCCTCAAAGCCTGCATCAAGAGCGGAGATTCATCGTTTTTGACCTTCCTTCTACGCAACAGCATACAACTTCATCGAAAGCATCAGCTTTGCCTCTATTAGGGGTAGTGGGACCCGAAGGAGGACTAACCGAGAGGGATTACCAAAGCTTTCCAAAGGGCTTTGAACGAGCTTCACTTTGA", "aa": "MLLSLPNKQEKLELIIQKFTEIGVDEIYLWVSERSVLKAINPNKLQRLSKIMQEATEQSWNRSLPKLSFIEAPQSLHQERRFIVFDLPSTQQHTTSSKASALPLLGVVGPEGGLTERDYQSFPKGFERASL", "aa_hexdigest": "b20c9e2c612647fe40f306972e032faf", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 650926, "stop": 651633, "observations": { "mutation": [ 650958, 650997, 651393 ], "stop": [ 650958, 650997, 651393 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EIX2", "query_cov": 1.0, "subject_cov": 0.5574468085106383, "identity": 0.9309999999999999, "score": 237.0, "evalue": 1.94e-77, "gene_start": 1, "gene_end": 131, "reference_start": 82, "reference_end": 212, "reference_sequence": "MQLFICPEFQVSGNQLIIKRNPELIQQLRKVLRAQMGYQFAVQAPEATERYFLRLQKRSDQELQAEIVQILPAPPKAPKTGMLLSLPNKQEKLELIIQKFAEIGIDEIFLRASERSVLKAINPNKLQRLSKIMQEATEQSWNRSLPKLSFIEAPQTLHQEWRFIVFDLPSTQQHTTLSKASALPLLGVVGPEGGLTERDYQSFPNGFERASLGDSVLRMETAAIIGAWKLKNLKN" }, "paralog": false, "identity": 0.9361702127659575, "score": 422.0, "evalue": 7.35e-153, "description": "Internal stop codon at 650958, 650997, 651393. Nonsense mutation around 650958, 650997, 651393.", "psc": { "uniref90_id": "UniRef90_A0A328EIX2", "product": "Ribosomal RNA small subunit methyltransferase E", "uniref50_id": "UniRef50_A0A660MSC0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EIX2", "UniRef:UniRef50_A0A660MSC0" ] }, "pscc": { "uniref50_id": "UniRef50_A0A660MSC0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MSC0" ], "product": "Ribosomal RNA small subunit methyltransferase E" } }, "id": "NKOCPLAKPK_1225", "locus": "GCA003260355_01189" }, { "type": "cds", "sequence": "contig_1", "start": 651391, "stop": 651633, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAGCTTTTTATCTGTCCAGAGTTTCAAGTCTCTGGAAATCAGCTTATCATTAAAAGGAATCCTGAACTTATTCAGCAGTTGAGAAAAGTGCTCAGGGCTCAAATGGGGTATCAATTTGCTGTTCAGGCTCCTGAGGCGACAGAGAGATATTTTCTCAGGCTCCAAAAGCGATCTGATCAAGAGCTCCATGCTGAAATCGTGCAAATCCTTCCAGCGCCTCCCAAAGCTCTAAAAACCTGA", "aa": "MQLFICPEFQVSGNQLIIKRNPELIQQLRKVLRAQMGYQFAVQAPEATERYFLRLQKRSDQELHAEIVQILPAPPKALKT", "aa_hexdigest": "63e8d7dda017ad099e27a0b9c9f0480c", "start_type": "ATG", "rbs_motif": "TTATT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9318.851699999996, "isoelectric_point": 9.649321556091309 }, "id": "NKOCPLAKPK_1226", "locus": "GCA003260355_01190" }, { "type": "cds", "sequence": "contig_1", "start": 651826, "stop": 652098, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MT28", "UniRef:UniRef90_A0A660MT28" ], "nt": "ATGGAAAAAGAACGTTTAAAAAAAATCATGCAAGAGGTAAGAGAGTATTATGGTCTCTCATACCAAGAGTTTGCTAGTCTTGTTGAAGTTGCATATTTCAATATGAGAGATCGGAAGGAGAAAATCCTGATTCATTGGAAGCTCCCTAATGGTGCATATCTAGATAAGATTGTTATCAGTCGATGTTGCCCTTATCGGAGTAGAAAATATATGATTTCTCTCTTTGAGGATGGATGCAATCTTTCAGTGCATACCTATTATTGGAAAATTTGA", "aa": "MEKERLKKIMQEVREYYGLSYQEFASLVEVAYFNMRDRKEKILIHWKLPNGAYLDKIVISRCCPYRSRKYMISLFEDGCNLSVHTYYWKI", "aa_hexdigest": "b24be6f2c523662b5e81fa03dd92fe44", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A660MT28", "query_cov": 1.0, "subject_cov": 0.8333333333333334, "identity": 0.978, "score": 186.0, "evalue": 1.47e-59, "valid": true, "uniref50_id": "UniRef50_A0A660MT28" }, "pscc": { "uniref50_id": "UniRef50_A0A660MT28", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MT28" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11027.802299999994, "isoelectric_point": 9.152270317077637 }, "id": "NKOCPLAKPK_1227", "locus": "GCA003260355_01191" }, { "type": "cds", "sequence": "contig_1", "start": 652219, "stop": 652815, "strand": "-", "frame": 1, "gene": null, "product": "50S ribosomal protein L7/L12", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DC0171D", "UniRef:UniRef100_A0A328EK89", "UniRef:UniRef50_A0A2W4KDK6", "UniRef:UniRef90_A0A660MSG5" ], "nt": "ATGCCAAAAACAAAAACAAACGAAGAGTTGATGAAAAATTATCATCAATTACTCCAGTTCGCTCAGTCTGCGATTGAATCTGCAGATGTTCAGCTCAAGAGAGCAAAACTTACATTGGATCAGCTTGCTTCTTTTGATCCAGAAAATCCAGAATCTCTTCTAGCTTACCTAGAGGAAGTAAGAGCAACGAATCCTGAAGAGCTAAAGGTTTATAAAGCTGAAGAGGGAGAAGTCATTGAAGGAGTATTTGACGGATATTATATGGTCGGAGCTGATCACAAGAAGTATCCAGTGCCAGTAAATTACTCTAGTAAGAGTAAATTGGTGCCAGGAGACGTAATGAAGCTCAAGATTCTAAATGACGGAAAGCTTCTCTTTAAGCTCATTAGACCTGTAGAAAGAAAACACTTAAAAGCAACCCTTATGAGGACTGACGACAATAAATATACCGCTAACACTGACAATGGAAAGATATTTTTCCTCAATCAAGCAGCTGTGAGCTTCTATTCAGGAACTCCAGGTGATGAACTCTATATCATAGTAAATGCACAGGAAGAAGGGGCATTTGCAGCTATCGAGGCAATCATCAAACAATAA", "aa": "MPKTKTNEELMKNYHQLLQFAQSAIESADVQLKRAKLTLDQLASFDPENPESLLAYLEEVRATNPEELKVYKAEEGEVIEGVFDGYYMVGADHKKYPVPVNYSSKSKLVPGDVMKLKILNDGKLLFKLIRPVERKHLKATLMRTDDNKYTANTDNGKIFFLNQAAVSFYSGTPGDELYIIVNAQEEGAFAAIEAIIKQ", "aa_hexdigest": "fc9e2587f22c7e98f8ad05e2f4c713c9", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000DC0171D", "uniref100_id": "UniRef100_A0A328EK89", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DC0171D", "UniRef:UniRef100_A0A328EK89" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EK89", "uniref90_id": "UniRef90_A0A660MSG5" }, "psc": { "uniref90_id": "UniRef90_A0A660MSG5", "product": "50S ribosomal protein L7/L12", "uniref50_id": "UniRef50_A0A2W4KDK6" }, "pscc": { "uniref50_id": "UniRef50_A0A2W4KDK6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4KDK6" ], "product": "50S ribosomal protein L7/L12" }, "genes": [], "id": "NKOCPLAKPK_1228", "locus": "GCA003260355_01192" }, { "type": "cds", "sequence": "contig_1", "start": 653023, "stop": 653295, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTCAAAATTTCTTATTCTCCAGATCTCAGAGGCGAGGTTCTTATTTCAGGGAGCAAGAATGCTGCACTTCCAATCATCGCGGCTAACTACCTGATTGATCAGCAAATTCAACTAGAAAATAAGCCTGATATTAGTGATGTTCGTTCGATTGAAAAGCTCGCAGAAGATGCACTCAGCAAGTCAACAAACTTCTTTGATCTTACCTCAGAACTCGCAACCAAATTTAGGGCATCTATCCTTTTAATTCCTGTTGGGCTCAAGAAATTTTGA", "aa": "MFKISYSPDLRGEVLISGSKNAALPIIAANYLIDQQIQLENKPDISDVRSIEKLAEDALSKSTNFFDLTSELATKFRASILLIPVGLKKF", "aa_hexdigest": "01bb03c2f73af83b05b36883f75668ab", "start_type": "ATG", "rbs_motif": "TTCTT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9971.43739999999, "isoelectric_point": 6.188481712341309 }, "id": "NKOCPLAKPK_1229", "locus": "GCA003260355_01193" }, { "type": "cds", "sequence": "contig_1", "start": 653365, "stop": 653718, "strand": "+", "frame": 1, "gene": null, "product": "Enolpyruvate transferase domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A9E5GQW0" ], "nt": "TTGCATAAAGCTGGGGTTGAAATAACTCATGGGCAGTTTAAAACTTTTAAAGTAGTCGGCAAGCCAAAGAAAAACATTATGCTCCAGGAATTTTCTGTCACTGCAACAGAAGCACTAATCACGTATCTTGCATTCTTGGATGATGTAGACTATGAAATCACCGTTTATCAGGTAGCAACTGAACCTCACGTCAAAAATCTGATTGATTTTCTCAATGCAGCGGGAGCTGATATTCGTATGGGGATAGATCATACCATTACTGTAAATCCAAAGAAAATAGCAATCAAAGAGCCTTCTTTTAAAATTATTAGCGATTATATCGAGGCGGGGACTTATTTTGCGATAGGGGCATGA", "aa": "MHKAGVEITHGQFKTFKVVGKPKKNIMLQEFSVTATEALITYLAFLDDVDYEITVYQVATEPHVKNLIDFLNAAGADIRMGIDHTITVNPKKIAIKEPSFKIISDYIEAGTYFAIGA", "aa_hexdigest": "acaa632aa27a23b82e04c280f957273b", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A9E5GQW0", "query_cov": 0.8205128205128205, "subject_cov": 0.9056603773584906, "identity": 0.635, "score": 127.0, "evalue": 8.53e-36, "valid": false, "product": "Enolpyruvate transferase domain-containing protein", "uniref50_id": "UniRef50_A0A9E5GQW0" }, "pscc": { "uniref50_id": "UniRef50_A0A9E5GQW0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A9E5GQW0" ], "product": "Enolpyruvate transferase domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_1230", "locus": "GCA003260355_01194" }, { "type": "cds", "sequence": "contig_1", "start": 653749, "stop": 653898, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAATGTTGACGATCTTTCAGCAATGTATAATATTGCGAGCAAGATTGGAATTAATTTTAAAATTCTTGACAAGCAAACACTTGTTGTCAATTCTTATAATAAAGCAAACTATAAAGCGGTAAAGCTTGAAATCAGAATTTTCCCTTGA", "aa": "MNVDDLSAMYNIASKIGINFKILDKQTLVVNSYNKANYKAVKLEIRIFP", "aa_hexdigest": "3cebe6298c6dddeebb5b14228bb79440", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5605.528900000001, "isoelectric_point": 9.456109809875489 }, "id": "NKOCPLAKPK_1231", "locus": "GCA003260355_01195" }, { "type": "cds", "sequence": "contig_1", "start": 654124, "stop": 654252, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTGCTTGCTGGTATTATGGCAAAGGGGGAAACGATGATTATGAACGAAGATATTATTGCTAGGGGGTATGCTGATATCCTTAATAAATTAAGCACTATTGGAGTAAAGATATCCGCGCTTCATTAG", "aa": "MVLAGIMAKGETMIMNEDIIARGYADILNKLSTIGVKISALH", "aa_hexdigest": "1211a57e81d0333a084a8359ddfceb2b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4503.375099999999, "isoelectric_point": 6.518261528015137 }, "id": "NKOCPLAKPK_1232", "locus": "GCA003260355_01196" }, { "type": "cds", "sequence": "contig_1", "start": 654303, "stop": 654875, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EH19", "UniRef:UniRef90_A0A328EH19" ], "nt": "TTGAAGGAAAAATCAAGAGAAAATATTGACATTTTATATATTTTAGATATAAAGAAAACCAAACTTAAAAATAGTAAAATTATGAATAAAAAACGAAAATTAAAAGTAAAAACTCTGATCGACGGCTTCATCAAATGGTTTTGTTGTTATTCTCTGAAACCCTCTTATGCATCAAAGAGAGCATTTTTCGCCCTCTATTACCAAGAGGAAGAGGACTCCTGTTTTGATTTGCCTTTCCCTGAGCTGAAAAGCAGCGAAAGAGGAGTAGAAATGCCTGAGAATATCCTCAAAATTTATCGTTTGCTGTTCAGACTCCAAAACGCAATCAACGATGCGATAGATCTCAGAGATTCTCTACTCAAAAGAGTGTCTCGACGCATCCCTAATTATGCGGGCGAAGCTTTCAAGGTGAATGAGAACTACAGAAAAGCGATCCAAAGAATAGAGGAGCTCTATGCAATCAAGGAAATTGCAGAGGCTTATCTAAGAAAACAGGTAAGAAAGCTGAATCCTTCTATCAAAACGATCGAACCAAGAAAAAAAGCAGGAAAGTATTTCCTGCGTGTCCGTTGA", "aa": "MKEKSRENIDILYILDIKKTKLKNSKIMNKKRKLKVKTLIDGFIKWFCCYSLKPSYASKRAFFALYYQEEEDSCFDLPFPELKSSERGVEMPENILKIYRLLFRLQNAINDAIDLRDSLLKRVSRRIPNYAGEAFKVNENYRKAIQRIEELYAIKEIAEAYLRKQVRKLNPSIKTIEPRKKAGKYFLRVR", "aa_hexdigest": "7cfae63e90f80878d0b5032d32c0a974", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A328EH19", "query_cov": 1.0, "subject_cov": 0.9743589743589743, "identity": 1.0, "score": 363.0, "evalue": 8.5e-127, "valid": true, "uniref50_id": "UniRef50_A0A328EH19" }, "pscc": { "uniref50_id": "UniRef50_A0A328EH19", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EH19" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 22699.469500000017, "isoelectric_point": 9.872511100769042 }, "id": "NKOCPLAKPK_1233", "locus": "GCA003260355_01197" }, { "type": "cds", "sequence": "contig_1", "start": 654981, "stop": 655262, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DC00CCE", "UniRef:UniRef100_A0A328EL20", "UniRef:UniRef50_A0A328EL20", "UniRef:UniRef90_A0A328EL20" ], "nt": "ATGAAAAAGGAAAAAATTTTAGTCGTTCACAGTTTACAGGATGCTCAGAGTCTGAATCCTGAACTGAATTCTTATGTGGTGATTCTAGGCTATACTCCGACGCTTACCGGCGAGTGGAAGAATTGTGAGGGCAGTTCTCTTCCGTCTTCTCTTGACGCGTATAAAGGAGAACCGGTAGTAATCGTCAAAATTACGCCTCAGAAAGTGAAATGCTATGCTTTCCCTCCCAGGAAATCGTATTGCTCGACTGGAACCTATCGTCAGGTACTTGAACGAATATAA", "aa": "MKKEKILVVHSLQDAQSLNPELNSYVVILGYTPTLTGEWKNCEGSSLPSSLDAYKGEPVVIVKITPQKVKCYAFPPRKSYCSTGTYRQVLERI", "aa_hexdigest": "0b6a2e1eb5e4ccbacf9de1fcf0a10f3a", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000DC00CCE", "uniref100_id": "UniRef100_A0A328EL20", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DC00CCE", "UniRef:UniRef100_A0A328EL20" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EL20", "uniref90_id": "UniRef90_A0A328EL20" }, "psc": { "uniref90_id": "UniRef90_A0A328EL20", "uniref50_id": "UniRef50_A0A328EL20" }, "pscc": { "uniref50_id": "UniRef50_A0A328EL20", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EL20" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10434.994499999992, "isoelectric_point": 9.030747413635254 }, "id": "NKOCPLAKPK_1234", "locus": "GCA003260355_01198" }, { "type": "cds", "sequence": "contig_1", "start": 655568, "stop": 655978, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01558.24" ], "nt": "GTGCTCGGTGACAAGGAATACGCCTCTATCATTAAAGGAGATAACAACTGCTTTTTCCTGTATATTTCTAATGAGGAGCAGCCCTTTATTCGTAGGCAGATTGATTATTTTCTCGCCTATGATCCCTATGCAGTGAGCAAGAATGAAAGCATCTACGATTTGAAAAAAGTCTTTATGATCAAAGATGAGAAGTGTAAATACAAAAACACCTTTACTCTTGGAGCGGCTCTCAAAATTTTTGGAATCAGCTTGGAGGAAGGGAAATCGCTCTTGGAGCAGCAATTCTCTGAAAAGGAGTTCTCCCCTGAGGTGATGAATCAGAATTTTTTGGATTTGGAAGCAGGTTATCAGTATATTATTCAAAACTGTGGTGAAGCCTGTGATATTATAAACTGCAGTAAAGCAATCTGA", "aa": "MLGDKEYASIIKGDNNCFFLYISNEEQPFIRRQIDYFLAYDPYAVSKNESIYDLKKVFMIKDEKCKYKNTFTLGAALKIFGISLEEGKSLLEQQFSEKEFSPEVMNQNFLDLEAGYQYIIQNCGEACDIINCSKAI", "aa_hexdigest": "ff0e22caac61fd3e04b6d6d95f03091b", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01558.24", "name": "POR", "length": 53, "aa_cov": 0.3897058823529412, "hmm_cov": 0.9433962264150944, "evalue": 5.369614529269243e-10, "score": 36.15513229370117, "start": 65, "stop": 117 } ], "seq_stats": { "molecular_weight": 15740.799299999999, "isoelectric_point": 4.720955848693847 }, "id": "NKOCPLAKPK_1235", "locus": "GCA003260355_01199" }, { "type": "cds", "sequence": "contig_1", "start": 655994, "stop": 656629, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTACGGAAACGAGCTCATTGCAAAGGGAGCAATCGCAAGCGGACTGGATTTTTACGCTGCCTATCCGATGACGCCAGCTTCCTCTATCATTGATGAGGTCGTAAAAAATCCGAAAGTAAGCTTCTTTCAGGGAGAAGATGAAATCGCAGTCAGTATGGCGATGCTCGGTGCGAAATTTGCTGGGAAAAGAGCGATGTGTGGAACCAGTGGAGGCGGATTTGCACTGATGACCGAGTCTATTTCCTTTTCAAATCAAGCGGAACTTGGGGGCGTTTATGTGCTTGCTCAGAGAGATGGTCCAAGTACTGGAACTCCGACTTTTACGGGACAGGGCGATGTGAATTATGCTTTGCATGCGAGTTTTGGAGACACGAAGCCGATCGTGCTTTATCCCTCTACTTTTGAGGAGGCGTATTCTTTCGCTGGGAAGGCTCTCAATTGGTCGGATCAGTATCAGCATCCTGTGATTCTCCTGAGTGATAAACAGCTTTCGGAGTCTTATTTGAGCATTGATCCGCACAAACTCCTTGTCGAACCGATAAAAAGAGGTAAAAAAGCTGACTCCACAGCCGAGGATCAGTTCAAAAGATACGAATTTAGTCCTGATGGGATTTCTCCTTATAGTGTGCCGTGA", "aa": "MYGNELIAKGAIASGLDFYAAYPMTPASSIIDEVVKNPKVSFFQGEDEIAVSMAMLGAKFAGKRAMCGTSGGGFALMTESISFSNQAELGGVYVLAQRDGPSTGTPTFTGQGDVNYALHASFGDTKPIVLYPSTFEEAYSFAGKALNWSDQYQHPVILLSDKQLSESYLSIDPHKLLVEPIKRGKKADSTAEDQFKRYEFSPDGISPYSVP", "aa_hexdigest": "2f9d0df1a75047883ea1e7e323c8c76c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 22739.3366, "isoelectric_point": 4.949732780456542 }, "id": "NKOCPLAKPK_1236", "locus": "GCA003260355_01200" }, { "type": "cds", "sequence": "contig_1", "start": 656702, "stop": 657106, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGGCAAAATGATGGAGAAAAGAGCTCAAAAACTGAAGACTTTCGTGGAACAAGAGTTTAATTCAGATTTTTATGGCTATGAAATCATCAATCCTGATGCGGAAATTTTCTATATCACGATGGGAATCAACCGTTATGCCCTAGAGCAACATATCAAAGACAAAGCGGGAATGGGGCTGATTGTCATTAAATCGCTCTCCCCTTTTGATCCGAGATTAAAGACTTTCCTTGAAGAACATGCAAAAAAGATTAAAAGGCTGATTTTTGTAGAAATGAATCAGAGTGGAATTTTGGAAGATTTGGTGCGTAAGGAATGTGAGCTGTATGGCGAGTGGAATAAAAAAATAGAGCATTTTAGAAAAATTACGCTCTATCCGTTCTTTGAGGAGGGAATTATTTAA", "aa": "MKGKMMEKRAQKLKTFVEQEFNSDFYGYEIINPDAEIFYITMGINRYALEQHIKDKAGMGLIVIKSLSPFDPRLKTFLEEHAKKIKRLIFVEMNQSGILEDLVRKECELYGEWNKKIEHFRKITLYPFFEEGII", "aa_hexdigest": "0f144670b0dee5469127eb4948cc0b71", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15960.508099999994, "isoelectric_point": 7.790375709533691 }, "id": "NKOCPLAKPK_1237", "locus": "GCA003260355_01201" }, { "type": "cds", "sequence": "contig_1", "start": 657781, "stop": 658176, "strand": "+", "frame": 1, "gene": null, "product": "Thiamine pyrophosphate enzyme TPP-binding domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A929SBS0", "UniRef:UniRef90_A0A328EH72" ], "nt": "ATGAGAACGAATTTAAATAAAATCCAGCGATGTCCAGGATGCGGAAACTTCCTCATTCACCTCGCGCTTAAACAAGCCCTTGCCGAGCTGAAAATCCCAACGCATAAAACCGTGATTGTAACTGGGATCGGCTGTAATAGTAAAATGAGCCAATATATGGAAGGCTACGGTGCAGAAACCCTGCATGGAAGAGGAATCCCTTTTGCAACAGGGGTAAAGCTCGCAAACCCTGATCTGACTGTGATTTCGGTTTCTGGAGATGGCGATAGTTATGGGATCGGACTTGGGCATCTGCTGCATGCTGCGAGGAGGAATCTGCCTTTTGTGCATATTACCTGTGATAATGAAAACTATGCTCTCACCACAGGACAAGCTTCTGCGACAACTCCCCTTTGA", "aa": "MRTNLNKIQRCPGCGNFLIHLALKQALAELKIPTHKTVIVTGIGCNSKMSQYMEGYGAETLHGRGIPFATGVKLANPDLTVISVSGDGDSYGIGLGHLLHAARRNLPFVHITCDNENYALTTGQASATTPL", "aa_hexdigest": "b47a262afd6ae8076fcdb7e5e1df6ad2", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A0G1DUH1", "query_cov": 0.8473282442748091, "subject_cov": 0.8222222222222222, "identity": 0.523, "score": 129.0, "evalue": 2.99e-36, "valid": false, "product": "Pyruvate ferredoxin/flavodoxin oxidoreductase, beta subunit", "uniref50_id": "UniRef50_A0A662S1D2" }, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 657781, "stop": 658383, "observations": { "mutation": [ 658174 ], "stop": [ 658174 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EH72", "query_cov": 1.0, "subject_cov": 0.655, "identity": 1.0, "score": 270.0, "evalue": 4.89e-91, "gene_start": 1, "gene_end": 131, "reference_start": 1, "reference_end": 131, "reference_sequence": "MRTNLNKIQRCPGCGNFLIHLALKQALAELKIPTHKTVIVTGIGCNSKMSQYMEGYGAETLHGRGIPFATGVKLANPDLTVISVSGDGDSYGIGLGHLLHAARRNLPFVHITCDNENYALTTGQASATTPLGAKTKSTPEGNTLPPLHPVHLVETAGCSFVKTVVDKDLKTLKETIMQAIQHEGFAHINVQQACPSWKRW" }, "paralog": false, "identity": 0.955, "score": 400.0, "evalue": 1.41e-144, "description": "Internal stop codon at 658174. Nonsense mutation around 658174.", "psc": { "uniref90_id": "UniRef90_A0A328EH72", "product": "Thiamine pyrophosphate enzyme TPP-binding domain-containing protein", "uniref50_id": "UniRef50_A0A929SBS0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EH72", "UniRef:UniRef50_A0A929SBS0" ] }, "pscc": { "uniref50_id": "UniRef50_A0A929SBS0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A929SBS0" ], "product": "Thiamine pyrophosphate enzyme TPP-binding domain-containing protein" } }, "id": "NKOCPLAKPK_1238", "locus": "GCA003260355_01202" }, { "type": "cds", "sequence": "contig_1", "start": 658177, "stop": 658383, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02775.26" ], "nt": "GTGAAAACCAAGTCTACGCCTGAGGGAAATCCTGTTCCTCCGCTCCATCCCGTGCATTTGGTGGAAACGGCGGGTTGTAGCTTTGTAAAATCGGTCATTGATAAAGACATGAAAACACTGAAAGAAACGATCGTTCAAGCGATTCAGCATTCAGGTTTTGCGCATATCAATGTTCAGCAAGCTTGTCCAAGCTGGAAAAGATGGTAG", "aa": "MKTKSTPEGNPVPPLHPVHLVETAGCSFVKSVIDKDMKTLKETIVQAIQHSGFAHINVQQACPSWKRW", "aa_hexdigest": "465b70374d9a3241b56599de8fb27dd1", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02775.26", "name": "TPP_enzyme_C", "length": 57, "aa_cov": 0.8382352941176471, "hmm_cov": 0.9473684210526315, "evalue": 2.9202665576974524e-06, "score": 23.6021728515625, "start": 2, "stop": 58 } ], "seq_stats": { "molecular_weight": 7578.731200000001, "isoelectric_point": 9.139956855773928 }, "id": "NKOCPLAKPK_1239", "locus": "GCA003260355_01203" }, { "type": "cds", "sequence": "contig_1", "start": 658407, "stop": 658574, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAATGACTTCTCTTTCTTCAGAGTTTTTCTTGATACCTGATATCAGTGAATCAGATTTTGATTTTATTCAAAAAATAATAGCATCAACAGATCTTTCCTATTATGAAATCCAAAAGCCACAGTTTATCGTCATAAAAAATAAAGAGGAAATCATTGCTTTTTGA", "aa": "MEMTSLSSEFFLIPDISESDFDFIQKIIASTDLSYYEIQKPQFIVIKNKEEIIAF", "aa_hexdigest": "0e5f484baa38d3c797f7c76080017104", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6454.324399999998, "isoelectric_point": 4.144210624694824 }, "id": "NKOCPLAKPK_1240", "locus": "GCA003260355_01204" }, { "type": "cds", "sequence": "contig_1", "start": 658891, "stop": 659061, "strand": "+", "frame": 1, "gene": "xapX", "product": "XapX domain protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_R9PK69" ], "nt": "ATGTCACAACTTCTTCAAATTCTTACAGCGCTCGGATCAGGGATTCTGATGGGAGCAATCTTTACCCTTCTTAAACTTCCACTTCCTGCTCCAAATGCACGAGCTGGAATTGCAGGTATCGCAGGGATTTTTATCGGCTCTTTGCTTGTCGGGCTTTTTATCAAGAAATAG", "aa": "MSQLLQILTALGSGILMGAIFTLLKLPLPAPNARAGIAGIAGIFIGSLLVGLFIKK", "aa_hexdigest": "8a5af66d4520e9af5ad5622037c251d5", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A396TZX3", "query_cov": 0.8035714285714286, "subject_cov": 0.8035714285714286, "identity": 0.578, "score": 56.6, "evalue": 1.47e-09, "valid": false, "product": "DUF1427 family protein", "uniref50_id": "UniRef50_R9PK69" }, "pscc": { "uniref50_id": "UniRef50_R9PK69", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_R9PK69" ], "product": "XapX domain protein" }, "genes": [ "xapX" ], "id": "NKOCPLAKPK_1241", "locus": "GCA003260355_01205" }, { "type": "cds", "sequence": "contig_1", "start": 659200, "stop": 659478, "strand": "+", "frame": 1, "gene": null, "product": "D-tyrosyl-tRNA(Tyr) deacylase", "db_xrefs": [ "EC:3.1.1.96", "KEGG:K07560", "SO:0001217", "UniRef:UniRef50_A0A328EIY3", "UniRef:UniRef90_A0A328EIY3" ], "nt": "ATGCGTGAAGATCGAAAGTCAGCACTAGAGAAATTCACGACCAAGATCCAAACACTCAAACTCCTCTCTTCTCCGAACGGGAAAATTGAAATTCCGCTTGCTGAAGGAGGAAATCAGATTTTGGTGATTTCAAATTTCACACTCTTTGGAAGCTATAAAAACGGTACGAAAATTGATTTTTCTCAGAGCGGGAAATTTAGTTTTTCTGAGGGAGCGTATGATTATTTTCTGGAAAGTCTACGTCGTAAAGGTTTTAGCGTAAAGAGTGGGAAATTCTGA", "aa": "MREDRKSALEKFTTKIQTLKLLSSPNGKIEIPLAEGGNQILVISNFTLFGSYKNGTKIDFSQSGKFSFSEGAYDYFLESLRRKGFSVKSGKF", "aa_hexdigest": "a130d4005bc0d8d2805724d56bccb09f", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 659065, "stop": 659538, "observations": { "mutation": [ 659155, 659161, 659476 ], "stop": [ 659155, 659161, 659476 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EIY3", "query_cov": 1.0, "subject_cov": 0.5859872611464968, "identity": 1.0, "score": 179.0, "evalue": 4.23e-56, "gene_start": 1, "gene_end": 92, "reference_start": 46, "reference_end": 137, "reference_sequence": "MKLIIQSVSSAQVEVYSDESYSERIKTEKIGSGILIYFGVGKTDEMREDRKSALEKFTTKIQTLKLLSSPNGKIEIPLAEGGNQILVISNFTLFGSYKNGTKIDFSQSGKFSFSEGAYDYFLESLRRKGFSVKSGKFGGDMLVSSINIGPINYIFEL" }, "paralog": false, "identity": 0.9808917197452229, "score": 294.0, "evalue": 4.93e-104, "description": "Internal stop codon at 659155, 659161, 659476. Nonsense mutation around 659155, 659161, 659476.", "psc": { "uniref90_id": "UniRef90_A0A328EIY3", "product": "D-tyrosyl-tRNA(Tyr) deacylase", "ec_ids": [ "3.1.1.96" ], "uniref50_id": "UniRef50_A0A328EIY3", "kegg_orthology_id": "K07560", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EIY3", "EC:3.1.1.96", "UniRef:UniRef50_A0A328EIY3", "KEGG:K07560" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EIY3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EIY3" ], "product": "D-tyrosyl-tRNA(Tyr) deacylase" } }, "id": "NKOCPLAKPK_1242", "locus": "GCA003260355_01206" }, { "type": "cds", "sequence": "contig_1", "start": 659574, "stop": 659882, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATCGACTGTCATTTCCCTCCTTTAGATCCAAAAAAAACCGTCCTTACCCTCAAGGTCAAGCATTTGGACTATGAAAAAATTGACGAAGCACTTGCGCCTGAACTCAGGACTCAGCTTTCAGACTTTAGACTCAAAAAAACTTCTGCAAAGGTTGATGATTTGCTGCTCCAGCTCCAAAAACCTGACAGCGACGCTTTGCAAACCTTCGGTGGCTGGAAGGACTTACTAAAAACCTTCCTCCATAGCCAATATAACGAGGACTATCCAAAATATGAAAAACTTTTGAGGGAATTGAAGATCATTTAA", "aa": "MIDCHFPPLDPKKTVLTLKVKHLDYEKIDEALAPELRTQLSDFRLKKTSAKVDDLLLQLQKPDSDALQTFGGWKDLLKTFLHSQYNEDYPKYEKLLRELKII", "aa_hexdigest": "5e91097687bf749900230a81675ec3d4", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11985.789099999989, "isoelectric_point": 6.824964714050293 }, "id": "NKOCPLAKPK_1243", "locus": "GCA003260355_01207" }, { "type": "cds", "sequence": "contig_1", "start": 660369, "stop": 660737, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00149.33" ], "nt": "TTGCTCGATCAGATCAAAGCTTTTGTTGCCTCACAGCCAGAAGAAAAAAATCTGATTTTTCTTGGGGATTTTGTCTATCATTTCAGCTATGATAGGGCAGCCTTGCTCGCACTCTTTGAACTCTTTCTAGAACTTTATACACAGGGCAAAAATCTCTACATTCTCGCAGGAAACCACGATCGGCTGGGCAACTCTTTCGTGTATGAAGAGGGGAAGCAGGTGTTTGATCTCCTCCAGCACCAGCAAAACTCAGGAAAGCTCCATTTCATCACGAAACCTCGGCTTACTGAGCTTGAGGGCGAGAAAATTTTGTTTCTTCCGTTCTGTTTGGATCTGCACGAAGAAGAATATTCAGCTTTTCCACTCTGA", "aa": "MLDQIKAFVASQPEEKNLIFLGDFVYHFSYDRAALLALFELFLELYTQGKNLYILAGNHDRLGNSFVYEEGKQVFDLLQHQQNSGKLHFITKPRLTELEGEKILFLPFCLDLHEEEYSAFPL", "aa_hexdigest": "234d70fab5b7258dafcba0aa8bf449dc", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00149.33", "name": "Metallophos", "length": 94, "aa_cov": 0.6065573770491803, "hmm_cov": 0.8829787234042553, "evalue": 2.5254739260643476e-08, "score": 31.010028839111328, "start": 8, "stop": 81 } ], "seq_stats": { "molecular_weight": 14232.091099999985, "isoelectric_point": 4.971274757385253 }, "id": "NKOCPLAKPK_1244", "locus": "GCA003260355_01208" }, { "type": "cds", "sequence": "contig_1", "start": 661163, "stop": 661741, "strand": "-", "frame": 3, "gene": null, "product": "DUF4300 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328ELM9", "UniRef:UniRef90_A0A328ELM9" ], "nt": "TTGACGCCAGAGTATGATAGCGACTTTTATCAGCAGCAACGAAATAACATACACCAAGATTTTATGGGCTACAATTGTAGAATCACAGCATTCTCGCTTTTACAAGACGCGATCAAGATAAAAGACGTCCCAGAGACCGAAATCCCTGAAAATCTAATGTTTGATATGCCTGGGTTTCGCTCCATACAGTCCTACACAAAAGAAAAGGCTAATAAGTTTGTAAACCTTTTCGCTCAAATCCCGACAACCGCTACAGCAACCCAAACAGAAAATATCCAAAAAGTAAAAGATACGCTCGCTAAAAAAGGCGTTGAGTTCCTCAATACAAAAGCAAGCTTGATCTCAGTATTTTTCCACGATGTGATTGATCCAAGCACGGCAAACCTTTTCATCGGTCATATTGGAGTTCTCGTGCCTTCCGAAAACGGCGAACTTCTGTTTATCGAAAAAATAGCTTTTGATCAACCCTACCAAGCGGTTAAATTCAAAAATAGAGCAGAACTCAATGGTTATTTAATGGGGAAATACGACGTCGATTATTCTGGAGAAGGTTCCAGACCGTTCATCTTCGAAAACTGA", "aa": "MTPEYDSDFYQQQRNNIHQDFMGYNCRITAFSLLQDAIKIKDVPETEIPENLMFDMPGFRSIQSYTKEKANKFVNLFAQIPTTATATQTENIQKVKDTLAKKGVEFLNTKASLISVFFHDVIDPSTANLFIGHIGVLVPSENGELLFIEKIAFDQPYQAVKFKNRAELNGYLMGKYDVDYSGEGSRPFIFEN", "aa_hexdigest": "06579e3289c0c3862d71aa6f29e09c55", "start_type": "TTG", "rbs_motif": "TTTA", "genes": [], "truncated": "both", "pseudogene": { "start": 661082, "stop": 662035, "observations": { "mutation": [ 661165, 661780, 661942, 661963 ], "stop": [ 661165, 661780, 661942, 661963 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328ELM9", "query_cov": 1.0, "subject_cov": 0.5853658536585366, "identity": 0.9690000000000001, "score": 377.0, "evalue": 3.22e-130, "gene_start": 1, "gene_end": 192, "reference_start": 110, "reference_end": 301, "reference_sequence": "MKKLALLCSLGIIIAGCSTQIDQAATTTDTTQTQSGIIATETGTQSYVQLTGIRYSNMTDAQSQAEVKNALLKADVSQTAVQAFFAMVDDFTKIVEGKGLNHGFTRAETLTPEYDSDFYQQQRNNIHQDFMGYNCRITAFSLLQDAIKIKDAPETEIPENLMFDMPGFRSIQSYTKEKDRKFVNFFAQIPTTATATQTENIQKVKDTLAQKGVEFLNTKASLISVFFHDVIDPSTANLFIGHIGVLVPSENGELLFIEKIAFDQPYQAVKFKNRAELNGYLMGKYDVDYSGEGSRPFIFENGSLMADFQLNPATTQQLKANLQSPTSE" }, "paralog": false, "identity": 0.9589905362776026, "score": 593.0, "evalue": 1.19e-217, "description": "Internal stop codon at 661165, 661780, 661942, 661963. Nonsense mutation around 661165, 661780, 661942, 661963.", "psc": { "uniref90_id": "UniRef90_A0A328ELM9", "product": "DUF4300 domain-containing protein", "uniref50_id": "UniRef50_A0A328ELM9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328ELM9", "UniRef:UniRef50_A0A328ELM9" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328ELM9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328ELM9" ], "product": "DUF4300 domain-containing protein" } }, "id": "NKOCPLAKPK_1245", "locus": "GCA003260355_01209" }, { "type": "cds", "sequence": "contig_1", "start": 661778, "stop": 661897, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACGGATGCTCAATCTCAAGCAGAGGTCAAAAACGCACTCCTCAAGGCTGATGTGAGCCAAACAGCGGTCCAAGCTTTTTTTGCGATGGTAGATGATTTTACCAAGATTGTAGAGTGA", "aa": "MTDAQSQAEVKNALLKADVSQTAVQAFFAMVDDFTKIVE", "aa_hexdigest": "a034a1a7302743806924d1b01b3123dc", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4261.781999999998, "isoelectric_point": 4.394813728332521 }, "id": "NKOCPLAKPK_1246", "locus": "GCA003260355_01210" }, { "type": "cds", "sequence": "contig_1", "start": 662141, "stop": 662449, "strand": "-", "frame": 3, "gene": null, "product": "DUF4298 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DC00CE7", "UniRef:UniRef100_A0A328EK94", "UniRef:UniRef50_A0A328EK94", "UniRef:UniRef90_A0A328EK94" ], "nt": "ATGCATCAAAGACAGCTTCAAAAAATCCAGAAGATGGAAGCCATCCTCAATGAAATGAATCAAACTTTGGAAGAAGTCAATGTTGCATTTGAGAAGCGAAAAGCCCTAAGACCGCAGATCAAAGAATTGCTCAAGTATTACGAAAGTAAAGCTCGGTTTAGAGATGCTGAGGCGAGTAATAGAGGAGAACTTCCCGAAGATATGCCACATGGAGTGTTGAGTGAAGATGGAGCTTGGAATGCTTTTGTCTGTGAATATCAGCTTGCAAAACAACTTCAAAAATTTACAAAAGCAGTCCTCAAAAGGTAA", "aa": "MHQRQLQKIQKMEAILNEMNQTLEEVNVAFEKRKALRPQIKELLKYYESKARFRDAEASNRGELPEDMPHGVLSEDGAWNAFVCEYQLAKQLQKFTKAVLKR", "aa_hexdigest": "de2a90c28d7619a2f2e693976f4b8ad9", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000DC00CE7", "uniref100_id": "UniRef100_A0A328EK94", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DC00CE7", "UniRef:UniRef100_A0A328EK94" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EK94", "uniref90_id": "UniRef90_A0A328EK94" }, "psc": { "uniref90_id": "UniRef90_A0A328EK94", "product": "DUF4298 domain-containing protein", "uniref50_id": "UniRef50_A0A328EK94" }, "pscc": { "uniref50_id": "UniRef50_A0A328EK94", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EK94" ], "product": "DUF4298 domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_1247", "locus": "GCA003260355_01211" }, { "type": "cds", "sequence": "contig_1", "start": 662513, "stop": 662731, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DC05200", "UniRef:UniRef100_A0A328EKL1", "UniRef:UniRef50_A0A660MWJ1", "UniRef:UniRef90_A0A660MWJ1" ], "nt": "ATGAATAGTCCCGAAATAAACAAGGATCGTATGATTACTATCAAAACCTCTGAAGGGGAAATAAAAGTTTCTAGCAAAGAAATTGATGAAGGTTGGAATAGGGTAATGTATGGAGACAGAATAAAAAATACTCTTCAAGCTCCCTCTGAAAAAGGATCACTACATCAAAAGATAGGAACTTGGAAGCTTTGGCTTATGAATCTGCTGCGCGGTTTTTAA", "aa": "MNSPEINKDRMITIKTSEGEIKVSSKEIDEGWNRVMYGDRIKNTLQAPSEKGSLHQKIGTWKLWLMNLLRGF", "aa_hexdigest": "9f3344d8becf63f402097d37d0c4b113", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000DC05200", "uniref100_id": "UniRef100_A0A328EKL1", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DC05200", "UniRef:UniRef100_A0A328EKL1" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EKL1", "uniref90_id": "UniRef90_A0A660MWJ1" }, "psc": { "uniref90_id": "UniRef90_A0A660MWJ1", "uniref50_id": "UniRef50_A0A660MWJ1" }, "pscc": { "uniref50_id": "UniRef50_A0A660MWJ1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MWJ1" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8349.560399999998, "isoelectric_point": 9.453853416442872 }, "id": "NKOCPLAKPK_1248", "locus": "GCA003260355_01212" }, { "type": "cds", "sequence": "contig_1", "start": 662915, "stop": 663214, "strand": "-", "frame": 3, "gene": null, "product": "Nudix hydrolase domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EI01", "UniRef:UniRef90_A0A328EI01" ], "nt": "TTGATAGAGGAAACAGGGCTCCAAAACGTTGCTTTACATAATTGGCATAAAGAGCATCATGGTATCCCTCTGGATATTGATACTCACGCCATTCCTTATAGCGAAAAGAAACAAGAACCCGCTCACTTTCACCATGATTTCAGGTTTGTCTTTGTGCTGACAGGAGAGCAAAAGCTGACCTTACAGAGAGAAGAAGTCGCTGAATTTGGACGAAAAGCTTGTGATACACTTCCTGAAGAGGCCTCGAGTCTGCTAATCAAAAAGAAATTACTGCAGATCAAAGAATTGCTCAAGTATTAG", "aa": "MIEETGLQNVALHNWHKEHHGIPLDIDTHAIPYSEKKQEPAHFHHDFRFVFVLTGEQKLTLQREEVAEFGRKACDTLPEEASSLLIKKKLLQIKELLKY", "aa_hexdigest": "ddeb7037b4d4727fa0a7e94d6d8ef91e", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 662915, "stop": 663514, "observations": { "mutation": [ 663268, 663502 ], "stop": [ 663268, 663502 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EI01", "query_cov": 1.0, "subject_cov": 0.4925373134328358, "identity": 0.929, "score": 191.0, "evalue": 4.38e-60, "gene_start": 1, "gene_end": 99, "reference_start": 103, "reference_end": 201, "reference_sequence": "MQQEKTGTFRIDLSAYVEETLKRYLHTFPSEESRFSLLKTQLQNAENLGTRKNFNGHMTASAYLLSEGKCLMIHHIALDKRLAPGGHWEDGDGELFHTAQRELIEETGVQNVALHNWHKEHHGIPLDIDTHAIPYSEKKQEPAHFHHDFRFVFVLTGEQKLTLQREEVADFGRKTYDALPEDASSLLIKKKLLQIKELLKY" }, "paralog": false, "identity": 0.9447236180904522, "score": 385.0, "evalue": 2.62e-139, "description": "Internal stop codon at 663268, 663502. Nonsense mutation around 663268, 663502.", "psc": { "uniref90_id": "UniRef90_A0A328EI01", "product": "Nudix hydrolase domain-containing protein", "uniref50_id": "UniRef50_A0A328EI01", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EI01", "UniRef:UniRef50_A0A328EI01" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EI01", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EI01" ], "product": "Nudix hydrolase domain-containing protein" } }, "id": "NKOCPLAKPK_1249", "locus": "GCA003260355_01213" }, { "type": "cds", "sequence": "contig_1", "start": 663266, "stop": 663472, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGAGGAAACCTTAAAACGCTATCTTCATACCTTCCCTTCAGAAGAATCCAGATTTTCTCTCTTAAAAACTCAGCTCCAGAACGCAGAAAATCTCGGAACAAGAAAAAACTTCAATGGACATATGACTGCAAGTGCATATCTGCTTTCTGAAGAGAAGTGTTTGATGATTCATCATATTGCTCTAGATAAACGGCTTGCTCCATGA", "aa": "MEETLKRYLHTFPSEESRFSLLKTQLQNAENLGTRKNFNGHMTASAYLLSEEKCLMIHHIALDKRLAP", "aa_hexdigest": "8c8571e76cd76bc5c7142640c333f4ce", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7904.006999999995, "isoelectric_point": 7.952642631530762 }, "id": "NKOCPLAKPK_1250", "locus": "GCA003260355_01214" }, { "type": "cds", "sequence": "contig_1", "start": 663475, "stop": 663648, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAGACAGATCAACGCGAAAGGTTCAGGTTTTCTCTTGTTGCATTTTTTATCGTGAAGGAAAGAATAAAAAAAGGAATAGATTTTTGGCTGAAAATAAAGTGCTTCCTTATCAAAGATTATCCAATCAGTTCCTCTTATCGTCGTAAGTTAAAAAATGAGGCTCTGTTTTAG", "aa": "MQTDQRERFRFSLVAFFIVKERIKKGIDFWLKIKCFLIKDYPISSSYRRKLKNEALF", "aa_hexdigest": "9916533770c31f8705ccb9205c7dd8f3", "start_type": "ATG", "rbs_motif": "TTTA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7049.315899999998, "isoelectric_point": 10.17177333831787 }, "id": "NKOCPLAKPK_1251", "locus": "GCA003260355_01215" }, { "type": "cds", "sequence": "contig_1", "start": 664216, "stop": 664377, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCTCCAACGATTAAGGTAGAAACAGTTTTAGATCAGTTTGTTTCTCCTTTAGATATGTCTATTATGAAGAGAGAAATAAAAATGATCAAAGAAGAGCTATCTGGACAGATTTCAGATACAACTCTTCTCGTAAATCGTATTGGGAAGGAAATATATTGA", "aa": "MPPTIKVETVLDQFVSPLDMSIMKREIKMIKEELSGQISDTTLLVNRIGKEIY", "aa_hexdigest": "d4c973bd8288575aef0b66a9c664cd81", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6080.160599999998, "isoelectric_point": 5.179930686950683 }, "id": "NKOCPLAKPK_1252", "locus": "GCA003260355_01216" }, { "type": "cds", "sequence": "contig_1", "start": 665362, "stop": 665631, "strand": "-", "frame": 1, "gene": null, "product": "Chloramphenicol acetyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2D0L1W2" ], "nt": "TTGAAAAATTTTATTCAGGATCCCAATATCATTATCGGTGATTATACCTATTATGACGATCCAGAAGGTATTGAGAATTTTGAGAAAAATGTTCTGTATCGTAGCCCCATGATAGATAGTAAACTTATTATTGGAAAGTTTTGTGCAATTGCAACTGGAGTGAAATTCATTATGAACGCAGCAAATCACAAGATTGATGGTTTTTCTAGTTATCCTTTTGGAATTTTTGGAGGAGACTGGATGAAAGCTCTCCAAAGTTCTCAAAATTGA", "aa": "MKNFIQDPNIIIGDYTYYDDPEGIENFEKNVLYRSPMIDSKLIIGKFCAIATGVKFIMNAANHKIDGFSSYPFGIFGGDWMKALQSSQN", "aa_hexdigest": "08ca5c41cfb7a0d07235efcc606d4679", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A5C9CR75", "query_cov": 0.9213483146067416, "subject_cov": 0.803921568627451, "identity": 0.6459999999999999, "score": 122.0, "evalue": 1.19e-34, "valid": false, "product": "Transferase hexapeptide repeat containing protein", "uniref50_id": "UniRef50_A0A2D0L1W2" }, "pscc": { "uniref50_id": "UniRef50_A0A2D0L1W2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2D0L1W2" ], "product": "Chloramphenicol acetyltransferase" }, "genes": [], "id": "NKOCPLAKPK_1253", "locus": "GCA003260355_01217" }, { "type": "cds", "sequence": "contig_1", "start": 665777, "stop": 666619, "strand": "-", "frame": 3, "gene": "serS", "product": "serine--tRNA ligase", "db_xrefs": [ "EC:6.1.1.11", "GO:0000166", "GO:0004828", "GO:0005524", "GO:0006434", "KEGG:K01875", "SO:0001217", "UniRef:UniRef50_A0A2W4J0W9", "UniRef:UniRef90_A0A2W4J0W9" ], "nt": "ATGAAAAAGCACGATATGATTGATATTGAGAGAGGAGTGAAGCTTGGAGGTGCGAGAAGCTATTTTCTCAAGGGAGATGGAATGCTGCTTGAGCAGGCTGTTTTGCAATATACTCTCAAAAAGATCGTAGAGAGGGGTTTTACTCCTATGCAGGTGCCAAATATTGTGAATCCTGAATGTTTACAGGGAACCGGTTACTTTCCAGGAGGAGAAGAAGACGCATATCGAATGGAGAGAGATGATCAGCGATTGATTGGGACTTCAGAGATTCCAGTGACGGCTTATCATATGAATGAAATCCTCAACGAAGATGATTTACCAAAAAAATACTGTGGATATTCAGCGTGTTATCGCCGTGAGGCAGGAACCTATGGGAAAGATACAGCAGGACTCTATAGAGTGCATCAATTCCAAAAGGTAGAGCAAGTCGTAGTTTTGCCTGCTGATGAGAAAATGTCTGAGCAGTATTATCGTCAGATTTTGCAAAATTCTATGGATATTCTCGCGGATTTGGAGCTTCCTCATCGTTTGCTTCAGCTTTGCAGTGGAGATTTGGCGATTGGAAAGTATAATTCTCATGACCTTGAATGCCGAATGCCTTCTAGAAATGCTTATGGAGAGACACATTCAGTGACCGCTTTTCTGGATTTTCAAGCAAGAAGACTGAATCTCAAATATCGCGATAAAGAAGGGAAAATCCAGTATTGCTACACAATGAATAATACGGCGATTGCGACACCAAGAGTGTTGATTGCAATTATAGAAAATAATCAGACTGCTGATGGAAAGATCAGAATTCCAAAAGTTTTGCAGCCATATATGGGGAAGGAAGTCATTGGATAG", "aa": "MKKHDMIDIERGVKLGGARSYFLKGDGMLLEQAVLQYTLKKIVERGFTPMQVPNIVNPECLQGTGYFPGGEEDAYRMERDDQRLIGTSEIPVTAYHMNEILNEDDLPKKYCGYSACYRREAGTYGKDTAGLYRVHQFQKVEQVVVLPADEKMSEQYYRQILQNSMDILADLELPHRLLQLCSGDLAIGKYNSHDLECRMPSRNAYGETHSVTAFLDFQARRLNLKYRDKEGKIQYCYTMNNTAIATPRVLIAIIENNQTADGKIRIPKVLQPYMGKEVIG", "aa_hexdigest": "f22fbcbaa32583a8c3f092fec1eed5ce", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A948FMT2", "query_cov": 0.8821428571428571, "subject_cov": 1.0, "identity": 0.8140000000000001, "score": 431.0, "evalue": 2.13e-151, "valid": false, "gene": "serS", "product": "serine--tRNA ligase", "ec_ids": [ "6.1.1.11" ], "uniref50_id": "UniRef50_A0A948FMT2", "kegg_orthology_id": "K01875", "go_ids": [ "GO:0000166", "GO:0004828", "GO:0005524", "GO:0006434" ] }, "genes": [ "serS" ], "truncated": "3-prime", "pseudogene": { "start": 665777, "stop": 666919, "observations": { "mutation": [ 666667 ], "stop": [ 666667 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A2W4J0W9", "query_cov": 1.0, "subject_cov": 0.681265206812652, "identity": 0.975, "score": 561.0, "evalue": 2.66e-200, "gene_start": 1, "gene_end": 280, "reference_start": 132, "reference_end": 411, "reference_sequence": "MIDLKKVREDIAGYKLICQKKGKQIAVEEVLALDDQRKALQLEIDQTKAQQKQLASQQDYEGAKALKAKIQELETQHSEVLKQLETQLLKMPNTALHPEVPEGKDDSENVVVQTFGEMPQFDFQPLDHIELMKKHDMIDIERGVKLAGARSYFLKGDGMLLEQAVLQYTLKKIVERGFTPMQVPNIVNPDCLLGTGYFPGGEEDAYRMERDDQRLIGTSEIPVTAYHMNEILSEEELPKKYCGYSACYRREAGTYGKDTAGLYRVHQFQKVEQVVVLPADEKMSEQYYRQILQNSMDILADLELPHRLLQLCSGDLAIGKYNSHDLECRMPSRNAYGETHSVTAFLDFQARRLNLKYRDKEGKIQYCYTMNNTAIATPRVLIAIIENNQTAEGKIRIPKVLQPYMGKEVIG" }, "paralog": false, "identity": 0.9315789473684211, "score": 719.0, "evalue": 9.18e-265, "description": "Internal stop codon at 666667. Nonsense mutation around 666667.", "psc": { "uniref90_id": "UniRef90_A0A2W4J0W9", "gene": "serS", "product": "serine--tRNA ligase", "ec_ids": [ "6.1.1.11" ], "uniref50_id": "UniRef50_A0A2W4J0W9", "kegg_orthology_id": "K01875", "go_ids": [ "GO:0000166", "GO:0004828", "GO:0005524", "GO:0006434" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A2W4J0W9", "EC:6.1.1.11", "UniRef:UniRef50_A0A2W4J0W9", "KEGG:K01875", "GO:0000166", "GO:0004828", "GO:0005524", "GO:0006434" ] }, "pscc": { "uniref50_id": "UniRef50_A0A2W4J0W9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4J0W9" ], "product": "Serine--tRNA ligase" } }, "id": "NKOCPLAKPK_1254", "locus": "GCA003260355_01218" }, { "type": "cds", "sequence": "contig_1", "start": 666665, "stop": 667012, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02403.27" ], "nt": "ATGATTGATCTTAAGAAAGTTCGTGAAGATATTGCAGGATATAAGCTGATTTGCCAGAAGAAAGGAAAGCATTTTGATATTGATGCGATTCTTGCTTTAGATGATAAAAGGAAAGCTCTCCAGCTTGAAATTGATCAAACCAAGGCTCAGCAAAAGCAACTCGCTGCTCAAAAGGATTATGAAGGTGCAAAAAACCTCAAAATTCAGATTCAAGAGTTGGAAACTCAACATACGCAGGTAGATACTGAACTTAAAAGCTGGCTCCTCAAAATGCCAAATACCGCTTTGCATCCTGAGGTTCCAGAGGGGAAAGATGATAGTGAGAATGTGGTGGTTCAGAATTTTTGA", "aa": "MIDLKKVREDIAGYKLICQKKGKHFDIDAILALDDKRKALQLEIDQTKAQQKQLAAQKDYEGAKNLKIQIQELETQHTQVDTELKSWLLKMPNTALHPEVPEGKDDSENVVVQNF", "aa_hexdigest": "a26fc5437e77cce52d01680f4d0293f4", "start_type": "ATG", "rbs_motif": "TTTA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02403.27", "name": "Seryl_tRNA_N", "length": 107, "aa_cov": 0.8173913043478261, "hmm_cov": 1.0, "evalue": 3.7931709218455434e-16, "score": 55.763893127441406, "start": 1, "stop": 94 } ], "seq_stats": { "molecular_weight": 13196.96999999999, "isoelectric_point": 5.909629631042479 }, "id": "NKOCPLAKPK_1255", "locus": "GCA003260355_01219" }, { "type": "cds", "sequence": "contig_1", "start": 667291, "stop": 667491, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTGTCAATTTGCAATTCACCGGAAATATCAGCGAAAAGCTGAAAACACTCATAGAAAAAACTGATGCCTTAGATTGGATAGACATTGAAGATAAGAGGCTCATTGTCGGAGAAAACAATCCTCAAGTGAAGGAACTCATCAGCGAAGCGAATAAAGATGCAGAAACGCATGCAAAAGAAGTTGCAAGCCAGCTTTGA", "aa": "MSVNLQFTGNISEKLKTLIEKTDALDWIDIEDKRLIVGENNPQVKELISEANKDAETHAKEVASQL", "aa_hexdigest": "d17fdd9ba9ad9f2fad661cb870266690", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7423.280600000001, "isoelectric_point": 4.6691186904907225 }, "id": "NKOCPLAKPK_1256", "locus": "GCA003260355_01220" }, { "type": "cds", "sequence": "contig_1", "start": 667522, "stop": 667839, "strand": "-", "frame": 1, "gene": null, "product": "SIMPL domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EKJ2", "UniRef:UniRef90_A0A328EKJ2" ], "nt": "ATGAAAGCGACTGACAGACGATTTATTGCAGGGGTTGCTGTTATCCTTTGCCTTGCAGGAATGTGGAGTATGCACGATTTCTCCAAAAATCGTAATCTGAATGGCATCAACATTATGGGTGTAGCATCCAGAGAAATCCCAGCAGATACCGTTCAGTTTAGGATTACACTGATTGGAACCGGAACTACAAAAGCTATTAGAAAGGAACTGGCTTTTAAGAGACTAGAAGAAGTAAAAAACTTTGTAGAAGGAGCGGGCTTTACCAATAGTAATTATGACGGAAGACGTGTTTTTGATACTGATCAATGTTATTTTTGA", "aa": "MKATDRRFIAGVAVILCLAGMWSMHDFSKNRNLNGINIMGVASREIPADTVQFRITLIGTGTTKAIRKELAFKRLEEVKNFVEGAGFTNSNYDGRRVFDTDQCYF", "aa_hexdigest": "0ab5c704ec4ee67e9229e53a5844bbba", "start_type": "ATG", "rbs_motif": "TTTTTA", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 667219, "stop": 667839, "observations": { "mutation": [ 667293, 667524 ], "stop": [ 667293, 667524 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EKJ2", "query_cov": 1.0, "subject_cov": 0.4430379746835443, "identity": 0.9520000000000001, "score": 207.0, "evalue": 7.2e-66, "gene_start": 1, "gene_end": 105, "reference_start": 1, "reference_end": 105, "reference_sequence": "MKATDRRFIAGIAVILCLAGMWSMHNFSKNRNLNGINIMGVASREIPADTVQFRITLIGTGTTKAIRKELAFKRLEEVKNLVEGASFINSNYDGRRVFDTDQCYFGGTEKEYPCTRMSVNLQFTGNISEKLKTFIEKTDDLDWVDIEDKRLIVGENNPQVKELISEANKDAETHAKEVASQLGVKLGKLIVYSENGFAPEYREYGVANRSFFEDEEGASENSGVFVRVNTYHTYAID" }, "paralog": false, "identity": 0.9514563106796117, "score": 392.0, "evalue": 2.17e-141, "description": "Internal stop codon at 667293, 667524. Nonsense mutation around 667293, 667524.", "psc": { "uniref90_id": "UniRef90_A0A328EKJ2", "product": "SIMPL domain-containing protein", "uniref50_id": "UniRef50_A0A328EKJ2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EKJ2", "UniRef:UniRef50_A0A328EKJ2" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EKJ2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EKJ2" ], "product": "SIMPL domain-containing protein" } }, "id": "NKOCPLAKPK_1257", "locus": "GCA003260355_01221" }, { "type": "cds", "sequence": "contig_1", "start": 668356, "stop": 668838, "strand": "+", "frame": 1, "gene": null, "product": "Lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EGS2", "UniRef:UniRef90_A0A328EGS2" ], "nt": "TTGTCATTTGGATTTTCTCTACTCCTTACTGGTTGCTGGCTCAAGACTGGAAATCCTTCTCAAGAACCACAACCTGAAATAGTAAAGCAGGAGCTTCCTGATCAGGAGATGTCTCCACTCCTTTCTAGTACATCGCTCGGTAACTATGAAGAGATTCTTGAAGAAGTAGTTAATTCTGAAGCTCCTTGCAGTGTTTGTGAAATTAAATCAACTCCTGAGGAGATCAAAACTCTTATTATGCAAGCAAAACAGAAGGAGCAAAACCTTCCACATCCTGAAACCGCATCAGCTGAAACCTATTCAGAATTTGATTTTTTGAAAGCTTATGATACCGTTTATAGTGGGAACACCTATCGTATTATACCAGCTGCAACAGGGAAGAACCTGCTAACAGTAACTCTCAATGATCAACCTGTTTTTAGCTTTGAAAGGATCTTTATTACCAAGGAACCTGTTAAGAATTTTTTCGTCAATGAAGCTTGA", "aa": "MSFGFSLLLTGCWLKTGNPSQEPQPEIVKQELPDQEMSPLLSSTSLGNYEEILEEVVNSEAPCSVCEIKSTPEEIKTLIMQAKQKEQNLPHPETASAETYSEFDFLKAYDTVYSGNTYRIIPAATGKNLLTVTLNDQPVFSFERIFITKEPVKNFFVNEA", "aa_hexdigest": "9b3bb11e12865ce8334276a8dc990db2", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 668332, "stop": 669141, "observations": { "mutation": [ 668344, 668836 ], "stop": [ 668344, 668836 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EGS2", "query_cov": 1.0, "subject_cov": 0.5263157894736842, "identity": 0.9940000000000001, "score": 316.0, "evalue": 5.91e-107, "gene_start": 1, "gene_end": 160, "reference_start": 9, "reference_end": 168, "reference_sequence": "MKRIGFLCLSFGFSLLLTGCWLKTGNPSQEPQPEIVKQELPDQEMSPLLSSTSLGNYEEILEEVVNSEAPCSVCEIKSTPEEIKTLIMQAKQKEQNLPHPETASAETYSEFDFLKAYDTVYSGNTYRIIPAATGKNLLTVTLNDQPVFSFERIFITKEPVKNFFVNEAGWWLLYDENSYYDESNPQDKQEMMAKQGKPWRETNVLVHNGEKQTQNADNFALYNFNGKTFYFLRKQAGDKLSYFYDGKVYQTDFDEIIEDTMGSDLENAFEPIVDEEKGFLLFWAKKGGELSVNLLKTKTLAKMP" }, "paralog": false, "identity": 0.9925650557620818, "score": 547.0, "evalue": 1.85e-200, "description": "Internal stop codon at 668344, 668836. Nonsense mutation around 668344, 668836.", "psc": { "uniref90_id": "UniRef90_A0A328EGS2", "product": "Lipoprotein", "uniref50_id": "UniRef50_A0A328EGS2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EGS2", "UniRef:UniRef50_A0A328EGS2" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EGS2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EGS2" ], "product": "Lipoprotein" } }, "id": "NKOCPLAKPK_1258", "locus": "GCA003260355_01222" }, { "type": "cds", "sequence": "contig_1", "start": 668905, "stop": 669246, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAAAACAAGGGAAACCCTGGAGAGAGACTAATGTTTTAGTCCATAATGGAGAAAAACAGACTCAAAATGCTGATAATTTTGCTCTTTATAATTTTAATGGGAAAACGTTCTATTTTTTGAGGAAGCAGGCTGGGGATAAGCTAAGTTATTTCTACGATGGGAAGGTTTATCAGACCGATTTTGATGAAATCATAGAAGATACTATGGGTTCAGATCTTGAAAATGCGTTCGAACCGATTGTGGACGAAGAAAAGGGATTCTTGCTCTTTTGGGCCAAAAAGGGTGGAGAACTCAGTGTTAACCTCTTGAAAACTAAAACTCTTGCGAAGATGCCATAA", "aa": "MAKQGKPWRETNVLVHNGEKQTQNADNFALYNFNGKTFYFLRKQAGDKLSYFYDGKVYQTDFDEIIEDTMGSDLENAFEPIVDEEKGFLLFWAKKGGELSVNLLKTKTLAKMP", "aa_hexdigest": "7a1677b3c592fe9b1d96d6c3fb714572", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13027.56909999999, "isoelectric_point": 5.40995807647705 }, "id": "NKOCPLAKPK_1259", "locus": "GCA003260355_01223" }, { "type": "cds", "sequence": "contig_1", "start": 669447, "stop": 669563, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTGAAAGCGTATAATATTGCACTTGATGAATATCACAAATTGGAAGATATCAACCGAACAACGCTAGAGTTGATTAAAAAGAGAGCTCTAAGCAGTAAAGCTCTTAGTTTGTAG", "aa": "MLKAYNIALDEYHKLEDINRTTLELIKKRALSSKALSL", "aa_hexdigest": "cd20d9c86c64d768de167a548250264b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4404.1368, "isoelectric_point": 9.22350788116455 }, "id": "NKOCPLAKPK_1260", "locus": "GCA003260355_01224" }, { "type": "cds", "sequence": "contig_1", "start": 669553, "stop": 669864, "strand": "-", "frame": 1, "gene": "cutA", "product": "Divalent-cation tolerance protein CutA", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBFB6DC", "UniRef:UniRef100_A0A328EIZ3", "UniRef:UniRef50_A0A288DGR9", "UniRef:UniRef90_A0A328EIZ3" ], "nt": "ATGAAAATCCTACTGATGACTTATCCAAATGACGGAAGAAAGCTCAAGCAACTCATCACCGCCTTACTCAAAAAGAAGCTGATCGCCTGTGCAAACGTAATGAACTACGCAAAATCCTACTATCTCCGAGAAGGAAAAATCAAAAAAGACGAAGAAAAACTGATTTTTATAAAATTTCCAAAAGAAAAAGAGTCAGAATTGCTTCCTTTTATCAAAAAACTGCATCCTTACGAGATTCCAGAACTGATCGTTTTGGCTCCAGACGCAGTGGATGCAGCCTACCTTAATCGGCTTCTTTCTGCTACAAACTAA", "aa": "MKILLMTYPNDGRKLKQLITALLKKKLIACANVMNYAKSYYLREGKIKKDEEKLIFIKFPKEKESELLPFIKKLHPYEIPELIVLAPDAVDAAYLNRLLSATN", "aa_hexdigest": "e53c313446f0a97fc99078c0d3ffd375", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000DBFB6DC", "uniref100_id": "UniRef100_A0A328EIZ3", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBFB6DC", "UniRef:UniRef100_A0A328EIZ3" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EIZ3", "uniref90_id": "UniRef90_A0A328EIZ3" }, "psc": { "uniref90_id": "UniRef90_A0A328EIZ3", "product": "Divalent-cation tolerance protein CutA", "uniref50_id": "UniRef50_A0A288DGR9" }, "pscc": { "uniref50_id": "UniRef50_A0A288DGR9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A288DGR9" ], "product": "CutA1 divalent ion tolerance protein" }, "genes": [ "cutA" ], "id": "NKOCPLAKPK_1261", "locus": "GCA003260355_01225" }, { "type": "cds", "sequence": "contig_1", "start": 669881, "stop": 670093, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A352SWP1" ], "nt": "ATGAAAGATAACCCTTATAACCTGATCCATTTTGCAGGAGAAAGCTATACCAAGACTGCTCCTTTGACGATCACTCCAAAAGAGGATTCGCTGCTTAGAGTCTTTATGGTCTTCAAAGCCTTACAAGAACCGATTGAAGTGAAAGCTCAGACTTTTGAACCTTTTGAGAGAAAGGGATTTACGGTCGTTGAGCGAGGGGGTTCTGAACTTTAG", "aa": "MKDNPYNLIHFAGESYTKTAPLTITPKEDSLLRVFMVFKALQEPIEVKAQTFEPFERKGFTVVERGGSEL", "aa_hexdigest": "5e0561acfe88c36a33f40b21660ea332", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00325F9E30", "query_cov": 0.9571428571428572, "subject_cov": 0.8170731707317073, "identity": 0.597, "score": 87.8, "evalue": 2.27e-21, "valid": false, "uniref50_id": "UniRef50_A0A352SWP1" }, "pscc": { "uniref50_id": "UniRef50_A0A352SWP1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A352SWP1" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7992.0986, "isoelectric_point": 5.751844596862793 }, "id": "NKOCPLAKPK_1262", "locus": "GCA003260355_01226" }, { "type": "cds", "sequence": "contig_1", "start": 670142, "stop": 670453, "strand": "-", "frame": 3, "gene": null, "product": "DUF2931 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1V5X1T6", "UniRef:UniRef90_A0A328EL56" ], "nt": "ATGGATCCTCCTGTGGCTTATAAGCCCGTAATTTACCTCTATCCGGAGCATAAGCAAGAAATCAAAGTTCAGCTTGACTATCAGGGAGAACTTATCGCTGATTATCCTGCCTATGATGAGAAGCTCAGGGGCTGGGAAGTAATCGCTTCTCCAGAGGGGACTTTGATCAATAAAACAGATGGTAAGGAATATAGCTATCTTTTCCGAGAAGGCAAACCAGCACAAAAGTCTGACTATGACCTCACCAAAGGCTTCGTAGTAAAAGGAAGCGAAACAAGGGCATTTCTTCAGCAAAAATTAGCAGAAATCTGA", "aa": "MDPPVAYKPVIYLYPEHKQEIKVQLDYQGELIADYPAYDEKLRGWEVIASPEGTLINKTDGKEYSYLFREGKPAQKSDYDLTKGFVVKGSETRAFLQQKLAEI", "aa_hexdigest": "c8029959ff8bd4f12cb7672ef53dfe6a", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 669881, "stop": 670453, "observations": { "mutation": [ 670144 ], "stop": [ 670144 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EL56", "query_cov": 1.0, "subject_cov": 0.5421052631578948, "identity": 1.0, "score": 213.0, "evalue": 7.72e-69, "gene_start": 1, "gene_end": 103, "reference_start": 1, "reference_end": 103, "reference_sequence": "MDPPVAYKPVIYLYPEHKQEIKVQLDYQGELIADYPAYDEKLRGWEVIASPEGTLINKTDGKEYSYLFREGKPAQKSDYDLTKGFVVKGSETRAFLQQKLAEIGLTPKEYNEFIVYRYPKMKDNPYNLIHFAGESYTKTAPLTITPKEDSLLRVFMVFKALQEPIEVKAQTFEPFERKGFTVVERGGSEL" }, "paralog": false, "identity": 0.9947368421052631, "score": 384.0, "evalue": 5.91e-139, "description": "Internal stop codon at 670144. Nonsense mutation around 670144.", "psc": { "uniref90_id": "UniRef90_A0A328EL56", "uniref50_id": "UniRef50_A0A1V5X1T6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EL56", "UniRef:UniRef50_A0A1V5X1T6" ] }, "pscc": { "uniref50_id": "UniRef50_A0A1V5X1T6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1V5X1T6" ], "product": "DUF2931 family protein" } }, "id": "NKOCPLAKPK_1263", "locus": "GCA003260355_01227" }, { "type": "cds", "sequence": "contig_1", "start": 670580, "stop": 670759, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01479.30", "PFAM:PF22421.1" ], "nt": "TTGGTACAATCTGGACTCGCAGAATCCAATGGAGAAGCGAAAAAACTGATCCAACAGGGAGCGATCTCAGTCAATGAGCAGAAAGTAAGCGATATTGCACAAAACTTTTCAAAAGCTGATGCCGTAAATAATGTTTTACTCCTAAAGAAAGGGAAAAAGTTCGCAATCGCTCAGTTTTAG", "aa": "MVQSGLAESNGEAKKLIQQGAISVNEQKVSDIAQNFSKADAVNNVLLLKKGKKFAIAQF", "aa_hexdigest": "30e75f55d6f52dc3060175f70111ded5", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01479.30", "name": "S4", "length": 38, "aa_cov": 0.6440677966101694, "hmm_cov": 1.0, "evalue": 1.3768867069371866e-08, "score": 30.859027862548828, "start": 2, "stop": 39 }, { "id": "PF22421.1", "name": "SYY_C-terminal", "length": 54, "aa_cov": 0.8983050847457628, "hmm_cov": 1.0, "evalue": 7.987234522848469e-13, "score": 44.60872268676758, "start": 1, "stop": 53 } ], "seq_stats": { "molecular_weight": 6319.2044000000005, "isoelectric_point": 9.523737144470214 }, "id": "NKOCPLAKPK_1264", "locus": "GCA003260355_01228" }, { "type": "cds", "sequence": "contig_1", "start": 670802, "stop": 671647, "strand": "-", "frame": 3, "gene": "tyrS", "product": "tyrosine--tRNA ligase", "db_xrefs": [ "EC:6.1.1.1", "GO:0000166", "GO:0004831", "GO:0005524", "GO:0006437", "KEGG:K01866", "SO:0001217", "UniRef:UniRef50_A0A288DLM3", "UniRef:UniRef90_A0A328EHL3" ], "nt": "ATGATCGGCGATCCAACAGGGAAAACTGAAGCCAGAGCTTTCCTTGGGCTTGAAGAACTCAGGCATAATCAAGAAGCAATTACCGTGCAAGTCGGTCAGATTTTGGAAAATTTAAAAAAACTGACAGGAAAGGACTTTCAGTTTGAAGTCGTGAATAATTACGATTTTTACAAGGATTATACGGTTTTTGACTGGTATCGCACGGTCGGAAAATATATCACGATCAATACCATGCTCAGCAAAGAATCTGTGAAAAAGAGAATGGAGAACCCTGAATCTGGGATTAGCTATACCGAGTTTAGCTATATGCTGTTGCAGGGAAATGATTTCGTGCATCTCTACGAAAACAACGGTGTAAAGCTTCAAATCGGAGGTTCTGACCAGCGAGGAAATATGGTTACCGGAACCGAAATGCTGAGGAAAAAAACCGAAGGAGAAGCCGAAAGTTATGTGATGACGATCCCTTTGATGATGGATTCCAGCGGGAAAAAGTTTGGAAAATCTGAAGGGAACGCGATTTGGCTTGATCAGACCAAAAATTCTCCTTATTTCGTCTACCAATACTTCATCAATACCGCAGATGCTGATGTGGAAAAGTATTTGAGAGCCTTTACTTTGCTAGAGATTCCAGAGATTGAGGCGATTATTGCACAGCATCAGGAGAAACCTGAACTCAGATACGGTCAGAAACAGCTTGCAAAGTATGTAATTCAAGTGCTCTTCGGAAAAGAGGCAAGTGAACAGGCGGAAAAGATTTCAGAATTCCTTTTTGGAACTGGAGAAAAGCTCGCCTTACTCAAGGCAATGTCTGCGGAGGAAATCAAAGCTCTTGCTCACGAAACCTGA", "aa": "MIGDPTGKTEARAFLGLEELRHNQEAITVQVGQILENLKKLTGKDFQFEVVNNYDFYKDYTVFDWYRTVGKYITINTMLSKESVKKRMENPESGISYTEFSYMLLQGNDFVHLYENNGVKLQIGGSDQRGNMVTGTEMLRKKTEGEAESYVMTIPLMMDSSGKKFGKSEGNAIWLDQTKNSPYFVYQYFINTADADVEKYLRAFTLLEIPEIEAIIAQHQEKPELRYGQKQLAKYVIQVLFGKEASEQAEKISEFLFGTGEKLALLKAMSAEEIKALAHET", "aa_hexdigest": "97608843544a8bb581386d15858c432b", "start_type": "ATG", "rbs_motif": null, "genes": [ "tyrS" ], "truncated": "both", "pseudogene": { "start": 670580, "stop": 671866, "observations": { "mutation": [ 670804, 671650 ], "stop": [ 670804, 671650 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EHL3", "query_cov": 1.0, "subject_cov": 0.6565420560747663, "identity": 0.925, "score": 520.0, "evalue": 1.98e-183, "gene_start": 1, "gene_end": 281, "reference_start": 74, "reference_end": 354, "reference_sequence": "MNLREELAARGLLYQMTDEKFFDLYEKGGEKFYCGYDPTADSLHLGHFLTFMAAVNFMKRGNTFVMIIGGATGMIGDPTGKDSARAFLGLEELRHNQEAITKQVGQILENLKKLTGKDFQFEVLNNYDFYKDFTVFDWYRTVGKYITINTMLSKESVKRRLENPESGITYTEFSYMLLQGNDFVHLYENHGVKLQIGGSDQRGNMVTGTEMLRKKTEGKAESYVMTIPLMMDSSGKKFGKSEGNAIWLDQTKNSPYFVYQYFINTADADVEKYLRAFTLLEISEIEAIIAEHQQKPELRYGQKQLAKYVIQVLFGKEASEQAEKISDFLFGNGDKLDLLKTLSAEELNALAHETGGAKLESDEVRILELLTQVGLAESNGEAKKLIQQGAISVNEQKITDIAQTFSKADATNGVLLVKRGKKFALGQF" }, "paralog": false, "identity": 0.9018691588785047, "score": 776.0, "evalue": 3.69e-287, "description": "Internal stop codon at 670804, 671650. Nonsense mutation around 670804, 671650.", "psc": { "uniref90_id": "UniRef90_A0A328EHL3", "gene": "tyrS", "product": "tyrosine--tRNA ligase", "ec_ids": [ "6.1.1.1" ], "uniref50_id": "UniRef50_A0A288DLM3", "kegg_orthology_id": "K01866", "go_ids": [ "GO:0000166", "GO:0004831", "GO:0005524", "GO:0006437" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EHL3", "EC:6.1.1.1", "UniRef:UniRef50_A0A288DLM3", "KEGG:K01866", "GO:0000166", "GO:0004831", "GO:0005524", "GO:0006437" ] }, "pscc": { "uniref50_id": "UniRef50_A0A288DLM3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A288DLM3" ], "product": "Tyrosine--tRNA ligase" } }, "id": "NKOCPLAKPK_1265", "locus": "GCA003260355_01229" }, { "type": "cds", "sequence": "contig_1", "start": 671648, "stop": 671866, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATCTTAGACAAGAACTTGAAGCCCGTGGCTTACTTTACCAAATGACCAACGAAAACTTTTTTGATTTGTATGAGAAGGGCGGTGAAAAGTTCTATTGTGGTTATGATCCTACGGCAGATTCGCTTCATCTAGGGCATTTCTTGACCTTTATGGCTGCTGTGAATTTTATGAAAAGAGGCAATACCTTCGTGATGCTGATCGGAGGTGCGACTTGA", "aa": "MNLRQELEARGLLYQMTNENFFDLYEKGGEKFYCGYDPTADSLHLGHFLTFMAAVNFMKRGNTFVMLIGGAT", "aa_hexdigest": "3447d008bf555d17e838ead85abfe096", "start_type": "ATG", "rbs_motif": "TTATT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8236.3537, "isoelectric_point": 5.49726276397705 }, "id": "NKOCPLAKPK_1266", "locus": "GCA003260355_01230" }, { "type": "cds", "sequence": "contig_1", "start": 672226, "stop": 672417, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGAGAAAGGCTTGAAGAGAAGAGCAATCAGGACTCGATCCAGAAAATCGCTAAAGATGACCAGCTTGATCATAAACACCCACAAGTGAAGGAAAAGATTGCTGAGGATTTTTCTGGTTCTCAAAAAGAAGGTTTAGAAAAGCCATCTCTTAATTCGCTTGATAGCAAGCTGGCTGAGATTTTTCCCTGA", "aa": "MGERLEEKSNQDSIQKIAKDDQLDHKHPQVKEKIAEDFSGSQKEGLEKPSLNSLDSKLAEIFP", "aa_hexdigest": "1a361385338e82dae67a738fbbfd63ed", "start_type": "ATG", "rbs_motif": "TTTA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7135.842799999997, "isoelectric_point": 5.041527748107909 }, "id": "NKOCPLAKPK_1267", "locus": "GCA003260355_01231" }, { "type": "cds", "sequence": "contig_1", "start": 672439, "stop": 672564, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAAAGAGATTCTCATCAAACTAAAAGAATTTTCTCAGGATTTTCTCTCTGATCTTCTTACCGTAAAGAGTGGAAAATCTCTCGGGAAAGAAAGATTCTGGTCAGGCAAGCGAGAAGAACTTTGA", "aa": "MKEILIKLKEFSQDFLSDLLTVKSGKSLGKERFWSGKREEL", "aa_hexdigest": "3887ebd8861b3f43941f465311cfc937", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4815.587799999999, "isoelectric_point": 9.223250007629392 }, "id": "NKOCPLAKPK_1268", "locus": "GCA003260355_01232" }, { "type": "cds", "sequence": "contig_1", "start": 672976, "stop": 673206, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAAGAGTTTTGAGGAGTGGGAAAAAATGCTCAAATGGTCTGAATCCAACAACGAAGAACTCAAGAAAAATGCTGAATCCATAAAGCGTGCAGTCCTTGAAAAAGGTGAACTTTCTGGAGAAGCACTTAAACAAGAGTTTGCAAAAATTGATGCTGCGTGGCAAAAAGAAGCAAAAAACGCCTATGGAATGCTTAATCGTAGCGAAGTGATAAGATCAGCTTTTGTCTGA", "aa": "MKSFEEWEKMLKWSESNNEELKKNAESIKRAVLEKGELSGEALKQEFAKIDAAWQKEAKNAYGMLNRSEVIRSAFV", "aa_hexdigest": "573d73dc887fc989605b06149055ac20", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8809.9283, "isoelectric_point": 6.326373100280762 }, "id": "NKOCPLAKPK_1269", "locus": "GCA003260355_01233" }, { "type": "cds", "sequence": "contig_1", "start": 673336, "stop": 673773, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATCTAAAAATTCACCCTCTCCTCAAAGATGCTGAAGAGTGGCTTGATGCGGTTCTTAGGGCGAATAGAGACTATGCAGCTACAGCAAAAAGAGCTTCTGAACGCCTTGCAAAAGCTTACGAAAAAGGCAAATATGAAGGAGGAGAAGCTCAAGTTATGGAAAGATTTTCTACGGGGATGATGCGTCCTCAAGTGAATGCATCTCCAGAAAAGCCATCTCCAGAAAAAGTTAAATTAGAGAGTGCCCAAGATATTGGGATACAACGTAACGCTTCCTTACAGCAAGCCCTCGCGTCTATTAGTGATGAAGATGTTGAATCTACGATTCCGAGAGCAACCTATCTCCAAGAGATAGCGGCGTTTATTCAAAGAGATAGTAGCCTTTCTGTTGAAGATAAAATTCGTCTTCCAGAACTTATTTTGGAGGGAAGTTGA", "aa": "MDLKIHPLLKDAEEWLDAVLRANRDYAATAKRASERLAKAYEKGKYEGGEAQVMERFSTGMMRPQVNASPEKPSPEKVKLESAQDIGIQRNASLQQALASISDEDVESTIPRATYLQEIAAFIQRDSSLSVEDKIRLPELILEGS", "aa_hexdigest": "d882c21ffd3dd3f398c428d698a89df1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16161.068599999991, "isoelectric_point": 5.043062400817871 }, "id": "NKOCPLAKPK_1270", "locus": "GCA003260355_01234" }, { "type": "cds", "sequence": "contig_1", "start": 673801, "stop": 674064, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATGGGATGCAAGTCTCGCAAAAAAAAACTTCCTCGCATCACAGAAAGAGAGCCTTCAACAAAAAATCTTAAAAACTATGCGTCAAAAAGCGAAAGAGTCAAGAGATCATTTCTTTACTCATGTTGGGCTTCCTCTTAAGTACGATTCCCAGCTTAGTAAGGCTTATGAAACAAGATATAAAAAAACTCCAGGGACTTATTCAGTTTTTACTGATGCCAATAATCCTAATGATCAGTATGTTTTCAATCATCGCTCTTGA", "aa": "MKWDASLAKKNFLASQKESLQQKILKTMRQKAKESRDHFFTHVGLPLKYDSQLSKAYETRYKKTPGTYSVFTDANNPNDQYVFNHRS", "aa_hexdigest": "0295efb71a9094091c075f8f40d7de58", "start_type": "ATG", "rbs_motif": "TTTA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10232.45889999999, "isoelectric_point": 9.84240436553955 }, "id": "NKOCPLAKPK_1271", "locus": "GCA003260355_01235" }, { "type": "cds", "sequence": "contig_1", "start": 674074, "stop": 674223, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGAATACTACGAAATTTAACCAGCAACAAAAAGAGATCAACTTTAATGCGACGAAATTTAAGGCGATTCTCCGCTTTCCGACACCAGCAGAACAAGTTCAAAATGCACTGAATATTAGCTCCTACCTTCCAAGCTTAAGACCTTGA", "aa": "MKNTTKFNQQQKEINFNATKFKAILRFPTPAEQVQNALNISSYLPSLRP", "aa_hexdigest": "d5faa66b95d9a58ffea4ee995996cd30", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5667.456300000001, "isoelectric_point": 10.288847923278805 }, "id": "NKOCPLAKPK_1272", "locus": "GCA003260355_01236" }, { "type": "cds", "sequence": "contig_1", "start": 674431, "stop": 674571, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTGTCCCACTCCTCAATACGCTTCCTCAATGTTCAATAGATGAACTAAGGACTGTTGATCGCTTTTTTTCGGAGTTAGAGGCGATGCTCAATGATAATTCAACACAGAAGGAGGGAACTCATGAAAAAAATGAATGA", "aa": "MLVPLLNTLPQCSIDELRTVDRFFSELEAMLNDNSTQKEGTHEKNE", "aa_hexdigest": "f7f68489fb4d2216afab2793d14961fc", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5307.898, "isoelectric_point": 4.5407194137573255 }, "id": "NKOCPLAKPK_1273", "locus": "GCA003260355_01237" }, { "type": "cds", "sequence": "contig_1", "start": 674680, "stop": 674934, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAAAAACCTCTCCCGGGAGCTACTGATGATCTAGAACATAGGATTTTGGATGTAAGGAAGATGAAGCTCCTTAGTTCCAACAACCTCTCTCATCTTCGTGCAGAACAGGCTAGCCAACAGCCTGATGGACAACGATATTCCAAGCTTACCAAAACAATTGCCAATAATTATATTAAGACCGCAGAAACAGAACAGCAACACGTAGAGCAACAGCAACTTGAAACCTTGGAAAAACTCATTTCTAACGGCTAA", "aa": "MEKPLPGATDDLEHRILDVRKMKLLSSNNLSHLRAEQASQQPDGQRYSKLTKTIANNYIKTAETEQQHVEQQQLETLEKLISNG", "aa_hexdigest": "d2148ec2550fea1c8cb7f32189e0afe6", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9599.660100000003, "isoelectric_point": 6.27158031463623 }, "id": "NKOCPLAKPK_1274", "locus": "GCA003260355_01238" }, { "type": "cds", "sequence": "contig_1", "start": 674971, "stop": 675156, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAATCTTTAGTGCAAAAATCATTCCTTTAAATGTTGCGAATGGAGACGGGATTATCGCAATCCTCAACGCAGAGCAGGCTTCTCAATATGGGATTACGCAGCATGATAAAATTTCACTCATCAGAAAGGGTGAAGAATATGTCGTAGATGTTGCTCTTTCTAGTGAATATGTAGAACCTTGA", "aa": "MEIFSAKIIPLNVANGDGIIAILNAEQASQYGITQHDKISLIRKGEEYVVDVALSSEYVEP", "aa_hexdigest": "dc81bc3b46e7db281e07fb2a0462d46f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6662.4854000000005, "isoelectric_point": 4.437329292297363 }, "id": "NKOCPLAKPK_1275", "locus": "GCA003260355_01239" }, { "type": "cds", "sequence": "contig_1", "start": 675229, "stop": 675459, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02885.22" ], "nt": "ATGAGAAATAATCCGCTTTCTTTGCAGGCAATCAGGAAAAAGCTGCTCGGAGAGAAAATCACGACCGAAGAAATCGATGCGATCGTGGAAGATATTCAGAATAATAAACTTTCTGATCTGATTTTGGCATATTATACAGCAACGAGCTTTTTTTATAAAACAGATCCTGAAGAACTGGCATATACGACGAAAGCGACTGCTTATACCGGAGATATGTACCGTTTTCCATGA", "aa": "MRNNPLSLQAIRKKLLGEKITTEEIDAIVEDIQNNKLSDLILAYYTATSFFYKTDPEELAYTTKATAYTGDMYRFP", "aa_hexdigest": "bae77f7e1627b9f5c9d9712fce8b96fc", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02885.22", "name": "Glycos_trans_3N", "length": 58, "aa_cov": 0.7631578947368421, "hmm_cov": 1.0, "evalue": 1.1289106524314093e-08, "score": 31.167137145996094, "start": 9, "stop": 66 } ], "seq_stats": { "molecular_weight": 8758.846499999992, "isoelectric_point": 4.957974433898924 }, "id": "NKOCPLAKPK_1276", "locus": "GCA003260355_01240" }, { "type": "cds", "sequence": "contig_1", "start": 675616, "stop": 676143, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGAAGTGAGTTAAAAAAATCTGAAATCATCAGGCTCGTAGAGAAATACGGATGCTGCCTCGCTCGAAATGGAAATTTGAACCTCGCACCGGCGAATGATAGAATCATCAAGGTCTCTGCTCCTCTGGGAATGGAACCGTATGCCAGGATGATTTCCAGTATCATGGCGAAGAATTACGCTATGGGAATTAACCACTGCCTGATTGATATTCCTATTGGACCGACCGCTAAAGTAAACAAACAGGAAGATGCCGAAAGAATTGCTCAACATTTCAAAAATATTGGAACCTCACTTGGAATTAAAACAGAAGTTGCGATAACCAAAGCTGAACAGCCGATTGGAAACGGGATTGGTGCGGTTTTGCAGGTAAGAGAAGTGTTGAGAGTGCTTCAGCAACACGAGTTAAGAGCTTCTGATCTGGAAGAGAAAGCATTGGATTTGGCGGCACAACTCCTCGTGCTTTGCGGAAAGAATGAAAAGTATGAAGAAGCGTATAGGCTTGCAAAGGAGACACTCCAGAACTGA", "aa": "MRSELKKSEIIRLVEKYGCCLARNGNLNLAPANDRIIKVSAPLGMEPYARMISSIMAKNYAMGINHCLIDIPIGPTAKVNKQEDAERIAQHFKNIGTSLGIKTEVAITKAEQPIGNGIGAVLQVREVLRVLQQHELRASDLEEKALDLAAQLLVLCGKNEKYEEAYRLAKETLQN", "aa_hexdigest": "f1ab843c060b5328fa40f5a9270fd942", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19380.365800000003, "isoelectric_point": 8.644130516052247 }, "id": "NKOCPLAKPK_1277", "locus": "GCA003260355_01241" }, { "type": "cds", "sequence": "contig_1", "start": 676159, "stop": 676323, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAGGAAATTATTTCTGCACAAGAAGGGAATCCTGATGTAGATTCAGAAGGATTGGTCTTGGGAAGTCAGACTTTTGATATTGTGAGTGAACGCGATGGAAAAGTAGAAGCGATTGATATGAAATATCTCAATATGCTAGCAAAGACGCTCGGTGCTCCGTGA", "aa": "MQEIISAQEGNPDVDSEGLVLGSQTFDIVSERDGKVEAIDMKYLNMLAKTLGAP", "aa_hexdigest": "9578304686a8b2b642c70da2666d1d53", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5825.510600000001, "isoelectric_point": 4.105446434020995 }, "id": "NKOCPLAKPK_1278", "locus": "GCA003260355_01242" }, { "type": "cds", "sequence": "contig_1", "start": 676477, "stop": 676782, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAATTACTATTTTTGATGGAAGCACTTTGGAAGAAGTGCTTTTTTTGTCTTTATCTTTGGAGGGAATTCTGTATACTGCGAGCTCGCTTTACATTGAAGGAAAGTTTTCTGATGCACATGATCTGATTTTTGTTTTTTTCTTTTGTGGCATTCTGAGGATTGGCAAGTCTGACAGTTTTGAGGATTTCGAGTTGGTGGGGACGAAGAATTGGTCGAATCCAACGAGGACTAATCGTGCTGCTCGCCCTTATGGTTCCTTTGGCAATGTGGATAGCAAGAGCCTTTCAGGGTATCGTGGCTGA", "aa": "MKITIFDGSTLEEVLFLSLSLEGILYTASSLYIEGKFSDAHDLIFVFFFCGILRIGKSDSFEDFELVGTKNWSNPTRTNRAARPYGSFGNVDSKSLSGYRG", "aa_hexdigest": "9b02586e48017de67df21adc5cc59f52", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11264.587099999984, "isoelectric_point": 5.257800102233887 }, "id": "NKOCPLAKPK_1279", "locus": "GCA003260355_01243" }, { "type": "cds", "sequence": "contig_1", "start": 676730, "stop": 676912, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTCCTTTGGCAATGTGGATAGCAAGAGCCTTTCAGGGTATCGTGGCTGAAATGCTTGCAAAGGCTGGGCTCTTTTTGCTCGGTTTTTTGACGGTTTCGTTCAATGTGATTCTGGTTTTTGAACTGTTAAATTTTGTGAGTAAAACGATTTTTCATACCGATATGAGAGGCATCGTATGA", "aa": "MVPLAMWIARAFQGIVAEMLAKAGLFLLGFLTVSFNVILVFELLNFVSKTIFHTDMRGIV", "aa_hexdigest": "d69a6e5308c738a9636e676b2bf85a9a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6702.108399999997, "isoelectric_point": 8.365111351013184 }, "id": "NKOCPLAKPK_1280", "locus": "GCA003260355_01244" }, { "type": "cds", "sequence": "contig_1", "start": 676931, "stop": 677620, "strand": "+", "frame": 2, "gene": null, "product": "Calcineurin-like phosphoesterase domain-containing protein", "db_xrefs": [ "KEGG:K07098", "SO:0001217", "UniRef:UniRef50_A0A660MV80", "UniRef:UniRef90_A0A660MV80" ], "nt": "GTGTTGATTGGGACCACCGGGTTTGCGATGCGGAGTGCAAGAGCGATCAAAGTCAAAACCGTCGAGATTCCGGTCACGAACCTGACGAAAGAAAGCACCATCCTCTATCTCTCGGATATTCATATCTCTCATACTAGTGATTTGCCGTTTTTGAAGAAAATTATTGCGCAACTGAACGCTTTGGAGGGAGAGTTTGTGGTGATTAATGGAGATTTTATTGATGGGAAGGGGTTTTCGGAAGCAGATCTTCAGCTCTTGAATAGTATCAACAAAGACGTCTATCTCACGCTTGGAAATCACGAAGCCTACGCAGGAAACGCATATGTAAAGCAACTTTTGAAAAATACAAAAATCAGACTCTTGGAGGACGAAGTGCTTCATGCCCACGGATGGGAACTGATTGGACTTGCTGATATGGCAGGATTTGATACTGAGGAGAACCATGAGCTTCTTGATCAAAAACTCGCTACCCTCCTCACTAACACGACAACACTCCCTAAACTCTTAATTCTTCACGAGCCGATTGGACCGCAAGTAGCAGAACAGCATGGGATCAATCTTCAACTGGCAGGGCACACTCATTATGGTCAGATTTTTCCTTTTACTCTCGCTGTAAAGATTGCGTTTCCTTATATTAAGGGGCTTTACCGTTTTGGTACGAATGCGTTGTTTGTGAGTTCTGGGATCTGA", "aa": "MLIGTTGFAMRSARAIKVKTVEIPVTNLTKESTILYLSDIHISHTSDLPFLKKIIAQLNALEGEFVVINGDFIDGKGFSEADLQLLNSINKDVYLTLGNHEAYAGNAYVKQLLKNTKIRLLEDEVLHAHGWELIGLADMAGFDTEENHELLDQKLATLLTNTTTLPKLLILHEPIGPQVAEQHGINLQLAGHTHYGQIFPFTLAVKIAFPYIKGLYRFGTNALFVSSGI", "aa_hexdigest": "55f2bb5d9137182be3631e39d1090fb7", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 676631, "stop": 677692, "observations": { "mutation": [ 676634, 676910, 677618 ], "stop": [ 676634, 676910, 677618 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A660MV80", "query_cov": 0.9956331877729258, "subject_cov": 0.5728643216080402, "identity": 0.987, "score": 442.0, "evalue": 4.52e-154, "gene_start": 2, "gene_end": 229, "reference_start": 147, "reference_end": 374, "reference_sequence": "MEALWKKCFFCLYLWREFCILRLRFTLKGGILMQMIGFLFFSFVAFGGLASLTVLRISSWWGRRIGRIQRGLIVLLALMVPLAMWIARAFQGIVAEMLAKAGLFLLGFLTVSLNVILIFELLNFVSKTIFHTDMRGIVGGSLLIIALIGTTGFAMRSARAIKVKTVEIPVTNLTKESTILYLSDIHISHTSDLPFLKKIIAQLNALEGDFVVINGDFIDGKGFSEADLQLLNSINKDVYLTLGNHEAYAGNAYVKQLLKNTKIRLLEDEVLHAHGWELIGLADMAGFDTEENQELLDQKLATLLTNTTTLPKLLILHEPIGPQVAEQHGINLQLAGHTHYGQIFPFTLAVKIAFPYIKGLYRFDTNALFVSSGIGTWGPTMRLGSRSELVVLKLIPKG" }, "paralog": false, "identity": 0.9745042492917847, "score": 669.0, "evalue": 4.79e-246, "description": "Internal stop codon at 676634, 676910, 677618. Nonsense mutation around 676634, 676910, 677618.", "psc": { "uniref90_id": "UniRef90_A0A660MV80", "product": "Calcineurin-like phosphoesterase domain-containing protein", "uniref50_id": "UniRef50_A0A660MV80", "kegg_orthology_id": "K07098", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A660MV80", "UniRef:UniRef50_A0A660MV80", "KEGG:K07098" ] }, "pscc": { "uniref50_id": "UniRef50_A0A660MV80", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MV80" ], "product": "Calcineurin-like phosphoesterase domain-containing protein" } }, "id": "NKOCPLAKPK_1281", "locus": "GCA003260355_01245" }, { "type": "cds", "sequence": "contig_1", "start": 677915, "stop": 678214, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATAGGTATCAATTCCCACATAATATCCATAAAGAAGTGAAGGTGATGAAAATGAAATATCATAATATTTTTTGGGTTGATCAGAAAACTGGATATACATATGACTATTACATCCAAAAGTTTTTATCGTTTGTCATTCAATTCCCCTTTGAAGGTTCTGCCCTACCCCGAGAGTCCTTTTTTGCAAATGAGAGGTATCTCCCGTATATCAAAGCACTACCCCTGTTAAGGTTTTTCCTGCTTCTGCTAGCAGATAGGGATGATCCAATCAATAATATGCCATTCCTGACAAATTAA", "aa": "MYRYQFPHNIHKEVKVMKMKYHNIFWVDQKTGYTYDYYIQKFLSFVIQFPFEGSALPRESFFANERYLPYIKALPLLRFFLLLLADRDDPINNMPFLTN", "aa_hexdigest": "80b079febeb78b6da6225e2ad514e3a7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12158.025099999983, "isoelectric_point": 9.155364799499512 }, "id": "NKOCPLAKPK_1282", "locus": "GCA003260355_01246" }, { "type": "cds", "sequence": "contig_1", "start": 678327, "stop": 678476, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGAGAGAAAATCCAGAGTATCAAAAACAAATTATGGATATGGAAGAAGGGCTTGCCAATACCCAATGCTATCAATTCATTCAACTACAAAGATTCCCAAGAAGAACGGAAGATATTAATATTTCTTCTCCAATAGATGGGATTTGA", "aa": "MKRENPEYQKQIMDMEEGLANTQCYQFIQLQRFPRRTEDINISSPIDGI", "aa_hexdigest": "7a6b7737e2b311bf4ad6ca976045b1e2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5876.592099999999, "isoelectric_point": 4.812637138366699 }, "id": "NKOCPLAKPK_1283", "locus": "GCA003260355_01247" }, { "type": "cds", "sequence": "contig_1", "start": 678876, "stop": 679136, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACTTTGTTACTTGCAATTCTTAAAAAACTGTTAACTATTTATGATTATTGGATGCAAATAAAGCAACTAGTTCGTCTGGGAATTATTATCCTAGTGTTAACTTTTTTATCTTCAATCTTTCCTCCAATGAAAAAACCTTTGATTTTTCTTTTCACTTTACTACTGGGACGAGGGATAAGCTTTGCTGCACCAAGTCCACAATATTTAGAACACCTTACAACACAAATTTTAAAACAACAAACCCTGACTACAACTTGA", "aa": "MTLLLAILKKLLTIYDYWMQIKQLVRLGIIILVLTFLSSIFPPMKKPLIFLFTLLLGRGISFAAPSPQYLEHLTTQILKQQTLTTT", "aa_hexdigest": "51c869134190def5a47c2279234de2bb", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9877.024699999987, "isoelectric_point": 10.092283821105958 }, "id": "NKOCPLAKPK_1284", "locus": "GCA003260355_01248" }, { "type": "cds", "sequence": "contig_1", "start": 679252, "stop": 679452, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAAGAAATATTTTTAGTAGTGGATATACTGTTTCTTGATTTTTATAGAGAGATATTTATATTCAATTCCATCTTTAGCTTTAAACATAAAAGTATGAAAAGGTATGCTGTAGGATTTCTTATTGTTGCTATGTTGCTCTTCAGTGGATGTGCAGTGAAGGAACAAACCATTCAAACAGGAGAAGTGCTTGAAACCTGA", "aa": "MQEIFLVVDILFLDFYREIFIFNSIFSFKHKSMKRYAVGFLIVAMLLFSGCAVKEQTIQTGEVLET", "aa_hexdigest": "90256f2465582b18123cb0b85cbdeac4", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7717.092600000001, "isoelectric_point": 5.643793678283691 }, "id": "NKOCPLAKPK_1285", "locus": "GCA003260355_01249" }, { "type": "cds", "sequence": "contig_1", "start": 679702, "stop": 679992, "strand": "+", "frame": 1, "gene": null, "product": "Lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EHF4", "UniRef:UniRef90_A0A328EHF4" ], "nt": "ATGAATCCAGATTCCATAACCGTCTTTAGAGGTAATATTTATCCTGCTGATGTTTACCAGGAGATTGGAGTTTCTAGATGCAAGTATCGAGATCCAGATCCATGTAAGACAGAGAAACAATCAAAAGAAGAAAGAGAAAGTCTAAGGGGAATCACTATAGGACATAATAATAATTTCTACTTTACAGCAAATAAGGATCTTACTCGGAAAATTGATGATCCTCATTATGAGTCACATTATAGCAAGAGAAAGGATGTCTTTGATACGATAGAGTTCTTTGATGTAAAGTAG", "aa": "MNPDSITVFRGNIYPADVYQEIGVSRCKYRDPDPCKTEKQSKEERESLRGITIGHNNNFYFTANKDLTRKIDDPHYESHYSKRKDVFDTIEFFDVK", "aa_hexdigest": "bb892f59089ff0f8644b3b7b6a3da888", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 679402, "stop": 679992, "observations": { "mutation": [ 679450, 679492, 679603 ], "stop": [ 679450, 679492, 679603 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EHF4", "query_cov": 1.0, "subject_cov": 0.4485981308411215, "identity": 1.0, "score": 202.0, "evalue": 1.6e-64, "gene_start": 1, "gene_end": 96, "reference_start": 119, "reference_end": 214, "reference_sequence": "MKRYAVGFLIVAMLLFSGCAVKEQTIQTGEVLETGETTQTVVITEANSGDIVTQTGDNLIYRNEVYGFQVKLGKARKGAQIAGVWGGKQNRSTIYFTLPYTYVREIDEEAVNPDNVKGMNPDSITVFRGNIYPADVYQEIGVSRCKYRDPDPCKTEKQSKEERESLRGITIGHNNNFYFTANKDLTRKIDDPHYESHYSKRKDVFDTIEFFDVK" }, "paralog": false, "identity": 0.9846938775510204, "score": 393.0, "evalue": 3.36e-142, "description": "Internal stop codon at 679450, 679492, 679603. Nonsense mutation around 679450, 679492, 679603.", "psc": { "uniref90_id": "UniRef90_A0A328EHF4", "product": "Lipoprotein", "uniref50_id": "UniRef50_A0A328EHF4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EHF4", "UniRef:UniRef50_A0A328EHF4" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EHF4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EHF4" ], "product": "Lipoprotein" } }, "id": "NKOCPLAKPK_1286", "locus": "GCA003260355_01250" }, { "type": "cds", "sequence": "contig_1", "start": 680051, "stop": 681130, "strand": "-", "frame": 3, "gene": null, "product": "Peptidase C51 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EKK4", "UniRef:UniRef90_A0A328EKK4" ], "nt": "ATGAAAGCAAAGAAGATTGCATCAGTATTGATGCTTATCGGAATGTTCGTGGTTGTAGCTCTCTTGAGCTCCTGCGAGAAACAAGAAGTGGAAGAAGTTGTAGTTACTTCTTCGGATCAACAAGCTGGAGAGCAAGCATCTCCTGATAGCTCGGTTGCTCTACGAGCGGCAACTCTGGCGAGTGATGTAACCTACTACTCGACACCCGGAGTTGGTGTGTGGACGGCTAACAGTTCTCGGAATTTACCGATAAAGGGGATTTGTGGAACTGCTGAAGGAGGTGTTATCAAGGCTCGAGTTACTCGACAAGTGAATGGAACCTTTGAAGTGGAGATTATCAAACAGAATCGAGGTTTCTTCCTGGGGATGGGAGTTGCCTATATTAAAGCAGGGTCTCCCTGTGGTGGGATTGCGAGTCAAAAGTCCTATTCGGCATTCTCACAAAGTATAAAACTTTCGTTCTCTGCAACATTTAACACGGGATATGTTCATTTCTATCCCATGGTAATAGATGGGAAGACTGGCGTTCGCTATTATGCAGAACCAATCCTTGTATATACTTATCCTTTATTCAATTCAAAAGGCTCGTATACTCAGGGGGAGGAACTTGCAACTGCTAATGGAGTTGTAGTAAAGGCTGCTGGGAAAGGGCTTTATGATGCAAATAAGCTGAGTGTTCAATGTACTGAATTTTGCAACCGCTATTATTCCCAAGTTTACAAGAAAAATATTGTAAATAAAGGAAAAAATGGAGGGCATGGAAGAGATTGGTATAGAGAAGCATCAGCAAAGGGCTTAGATGCCTTCCCTAATTCAGGATCAGTTGCACCAAGACCTGGGGATATCCTCTGCTTTACTGGTGGACCAGGTGGCTATGGGCATGTTGCTATCATCATGGAAGTAACAAATACTTATGTAAGGCTTGTACATCAAAATATGGGGCTTGTCGAAGATGGGAAACCTGAAACTGACTGGCATGAGCCCGTTGGTGCTAAGTTGGACTATGATTATCGGACGAAGACTATTTCTTCTCCTGAAAACTACACAGTAAAAGGGTGGTTACGAATGCCTATGTATTAG", "aa": "MKAKKIASVLMLIGMFVVVALLSSCEKQEVEEVVVTSSDQQAGEQASPDSSVALRAATLASDVTYYSTPGVGVWTANSSRNLPIKGICGTAEGGVIKARVTRQVNGTFEVEIIKQNRGFFLGMGVAYIKAGSPCGGIASQKSYSAFSQSIKLSFSATFNTGYVHFYPMVIDGKTGVRYYAEPILVYTYPLFNSKGSYTQGEELATANGVVVKAAGKGLYDANKLSVQCTEFCNRYYSQVYKKNIVNKGKNGGHGRDWYREASAKGLDAFPNSGSVAPRPGDILCFTGGPGGYGHVAIIMEVTNTYVRLVHQNMGLVEDGKPETDWHEPVGAKLDYDYRTKTISSPENYTVKGWLRMPMY", "aa_hexdigest": "bfb3a106ed6888f06a2e0b2ab8caf089", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A328EKK4", "query_cov": 1.0, "subject_cov": 0.9065656565656566, "identity": 0.9390000000000001, "score": 664.0, "evalue": 8.01e-240, "valid": true, "product": "Peptidase C51 domain-containing protein", "uniref50_id": "UniRef50_A0A328EKK4" }, "pscc": { "uniref50_id": "UniRef50_A0A328EKK4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EKK4" ], "product": "Peptidase C51 domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_1287", "locus": "GCA003260355_01251" }, { "type": "cds", "sequence": "contig_1", "start": 681244, "stop": 681435, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGCCTCTGTGTGGAATTTTCAAAAATCGTCATTTTACTCCAAACTATAGAGTGTGCTTTCTCTTAGAACATCATTTTAGTGGGAACTACAAGGATGGGGGGGGGGAGTCAGACTTCCTCTTTATTTCTTTTCTTCTTGAAAAACTAGCGAGAATAACTAAAGTGAGGAGGAACTTATTTTTTACTTAA", "aa": "MKPLCGIFKNRHFTPNYRVCFLLEHHFSGNYKDGGGESDFLFISFLLEKLARITKVRRNLFFT", "aa_hexdigest": "df294620086148b1bcf38138f93b7006", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7471.646799999997, "isoelectric_point": 9.693740272521975 }, "id": "NKOCPLAKPK_1288", "locus": "GCA003260355_01252" }, { "type": "cds", "sequence": "contig_1", "start": 681451, "stop": 682038, "strand": "+", "frame": 1, "gene": "nusA", "product": "Transcription termination factor NusA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A288DC90", "UniRef:UniRef90_A0A2W4KUE9" ], "nt": "ATGAAACTGGATGGAAAAAGTATTCAGGCAGCAATTATGCATCTTGTGGAAGAATATAGATTAGATCCCTATCAAGTGATGGAAATTATTAGAGCAGGGATCAAATCAGGATTTAAAAAGGATTTTCCAGAATTTAGGAAAGCAGAAATCGTCGTTAATGTCGAAAACGATGGGACAATCAGTATTTATAAGGCTTTGACTGTTGTGGAAGAGATTGAGGAAGAAGACACCCAGATTCTTTTGGCTGATGCGAGGAAAGAAAGGAAAGATATTGAGCTCGGAGAACAGTTGCTAATCAATATTACTCCTCAGAGCATGGAACTTTCAAGGATTGCCGCACAAGCCGCTGCACAGACGATCAAACAAGGGATCAAAAATATTGAAAGAGAAAGGTTTTATGACAAGTTTCAGGATAAAGAAGGTCAGCTCTTGAAAGCAAAGGTGATCAAAGTGCATACAGATAGCGTAATCCTGGATATTGAAGGGACAACGGTGGTGCTTCCTCCAGAGAGCCAGATTCCTAATAGACCTTATGAAGTAGGAGAAGAAATTTTTGTTTTCCTCAAGAAAATTAGTAAAGAAGCCTGA", "aa": "MKLDGKSIQAAIMHLVEEYRLDPYQVMEIIRAGIKSGFKKDFPEFRKAEIVVNVENDGTISIYKALTVVEEIEEEDTQILLADARKERKDIELGEQLLINITPQSMELSRIAAQAAAQTIKQGIKNIERERFYDKFQDKEGQLLKAKVIKVHTDSVILDIEGTTVVLPPESQIPNRPYEVGEEIFVFLKKISKEA", "aa_hexdigest": "f074f1b9aad97a0ffaecfa0d7425a1c8", "start_type": "ATG", "rbs_motif": null, "genes": [ "nusA" ], "truncated": "3-prime", "pseudogene": { "start": 681451, "stop": 682341, "observations": { "mutation": [ 682036 ], "stop": [ 682036 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A2W4KUE9", "query_cov": 1.0, "subject_cov": 0.5685131195335277, "identity": 0.9590000000000001, "score": 358.0, "evalue": 1.21e-122, "gene_start": 1, "gene_end": 195, "reference_start": 1, "reference_end": 195, "reference_sequence": "MKLDGKSIQAAIMHLVDEYRLDPYQVMEIIRSGIKSGFKKDYPEFRKAEIVVNVENDGAISIYKALTVVEEIEEEDTQILLADAKKERKDIEIGEQLLINITPASMELSRIAAQAAAQTIKQGIKNIERERFYDKFQDKEGQLLKAKVIKVHTDSVILDIEGTTVVLPPESQIPNRPYEIGEEIFVFLKKISKEAGSITLEITQSSADYVEAILKKIVPEFEEGIVNIEKIGRIAGKKTKILVSSSDESVDPVGVFVGHKGDRINTVLSLLDGEKVDFVELIEDPKELVIRLLKPAQVESVEFKDDTKVIARVEESQKPLAIGKGASNIKLASQLSGYQIEVI" }, "paralog": false, "identity": 0.9324324324324325, "score": 536.0, "evalue": 7.82e-195, "description": "Internal stop codon at 682036. Nonsense mutation around 682036.", "psc": { "uniref90_id": "UniRef90_A0A2W4KUE9", "product": "Transcription termination factor NusA", "uniref50_id": "UniRef50_A0A288DC90", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A2W4KUE9", "UniRef:UniRef50_A0A288DC90" ] }, "pscc": { "uniref50_id": "UniRef50_A0A288DC90", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A288DC90" ], "product": "Transcription termination/antitermination protein NusA" } }, "id": "NKOCPLAKPK_1289", "locus": "GCA003260355_01253" }, { "type": "cds", "sequence": "contig_1", "start": 682174, "stop": 682482, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF07650.23", "PFAM:PF13184.12" ], "nt": "TTGGTAGCTAGCACAGATGAGAGCATTGATCCAGTGGGAGTATTTGTCGGACATAAAGGAGACAGAATCAATACGGTACTTTCATTGCTTGATGGAGAAAAGGTTGATTTCGTAGAAGATATTGATGATCCAAAGGAATTGATCACAAGGCTTCTCAAACCTGCACAAGTAGAGAGTCTTGAGTTTAAGGACAACGGGAAAGTGACTGCGAGAGTAGAAGAATCACAGAAATCACTCGCTATCGGTAAAGGTGCCTGCAATATCAAGCTTGCTTCTCAGCTTAGCGGATATCAAATTGAAGTTATTTAA", "aa": "MVASTDESIDPVGVFVGHKGDRINTVLSLLDGEKVDFVEDIDDPKELITRLLKPAQVESLEFKDNGKVTARVEESQKSLAIGKGACNIKLASQLSGYQIEVI", "aa_hexdigest": "c853db70fc3c243b9c2671cac5d8c36d", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07650.23", "name": "KH_2", "length": 56, "aa_cov": 0.5392156862745098, "hmm_cov": 1.0, "evalue": 7.626371394376336e-07, "score": 25.373615264892578, "start": 47, "stop": 101 }, { "id": "PF13184.12", "name": "KH_5", "length": 59, "aa_cov": 0.5784313725490197, "hmm_cov": 1.0, "evalue": 5.916271697461153e-20, "score": 67.6798095703125, "start": 2, "stop": 60 } ], "seq_stats": { "molecular_weight": 11071.468299999991, "isoelectric_point": 4.6874208450317365 }, "id": "NKOCPLAKPK_1290", "locus": "GCA003260355_01254" }, { "type": "cds", "sequence": "contig_1", "start": 682668, "stop": 683453, "strand": "+", "frame": 3, "gene": "pflA", "product": "pyruvate formate-lyase-activating protein", "db_xrefs": [ "EC:1.97.1.4", "GO:0043365", "SO:0001217", "UniRef:UniRef50_A0A2G6CBK7", "UniRef:UniRef90_A0A660MP92" ], "nt": "GTGCTTTTTTATCGAGAAACGCTTTATCTCTCTATCCCTATTATTTACGCTATGCTCAACGTTCATTCTATAGAAACTTTTGCGACGCAAGATGGTCCTGGGATCAGATTGGTGGTTTTTTTGCAAGGGTGTATGTTTAGATGCCAGTATTGTCATAATCCGGACACGATTCCGCTTGAGAACGAGAATGCAAAGCACCGAACCGCAGATGATATTCTCCTCCAAGCAGTAAAGCAAAGAGAATACTTTGGAGAGAAGGGCGGGATTACCTTTTCTGGAGGAGAACCGCTTACCCAAGCAGCAGCCTTACTGCCTGTAGTCCAAAAATTGAAAGCTGAAGGGTTCCACATTTGTATTGATACGAATGGATTTTGGCAGACTCCAGAGGCAAAAGAGGTTTTGAGCTTGGCAGATCTGATTTTGCCTGATTTGAAGCATATCAACCCAGAAAAACATCTCAAGCTCGTAGGACAGAGCAACGAAAATACGTTGAAGACACTGGATTATCTCGACGAGATCAAGAAACCTTACTGGCTCAGGTATGTCCTCGTGCCTGGCTATACTGATGACGAAGAGGACCTCCATACACTAGGGAAGTATCTACAGACCAGAACTGCTATGGAAAGGATTGAGATTTTGCCGTATCATAATCTGGGAAAATCCAAGTGGGACAAGCTTGGATGGAAATATCCTTTGGAAGGTGTGCATGCAGCAACAAGCCAGAATCTCGATACTGCGAAAAGGATCCTGAGTCAGTATTCAGAGAAAGTGTTTGTGAGAGGTTAA", "aa": "MLFYRETLYLSIPIIYAMLNVHSIETFATQDGPGIRLVVFLQGCMFRCQYCHNPDTIPLENENAKHRTADDILLQAVKQREYFGEKGGITFSGGEPLTQAAALLPVVQKLKAEGFHICIDTNGFWQTPEAKEVLSLADLILPDLKHINPEKHLKLVGQSNENTLKTLDYLDEIKKPYWLRYVLVPGYTDDEEDLHTLGKYLQTRTAMERIEILPYHNLGKSKWDKLGWKYPLEGVHAATSQNLDTAKRILSQYSEKVFVRG", "aa_hexdigest": "685f38e10e97a2d2fb3ef000d0e3aa15", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A660MP92", "query_cov": 0.9348659003831418, "subject_cov": 1.0, "identity": 0.992, "score": 509.0, "evalue": 2.08e-182, "valid": true, "gene": "pflA", "product": "pyruvate formate-lyase-activating protein", "ec_ids": [ "1.97.1.4" ], "uniref50_id": "UniRef50_A0A2G6CBK7", "go_ids": [ "GO:0043365" ] }, "pscc": { "uniref50_id": "UniRef50_A0A2G6CBK7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2G6CBK7" ], "product": "Pyruvate formate-lyase-activating enzyme" }, "genes": [ "pflA" ], "id": "NKOCPLAKPK_1291", "locus": "GCA003260355_01255" }, { "type": "cds", "sequence": "contig_1", "start": 683497, "stop": 683706, "strand": "+", "frame": 1, "gene": "copG", "product": "CopG family transcriptional regulator", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DC0062F", "UniRef:UniRef100_A0A328EHM2", "UniRef:UniRef50_A0A2W4IKK4", "UniRef:UniRef90_A0A660MRV6" ], "nt": "ATGAAAATGCAAGCCCTTGAAATCAGCGAACGTAGCTACCAAGAATTTACGAAGCTCAAAAAAATTATTGAAGCAATGGCAGGAGAAACCGTTTCTGATGATCAGGTTTTTGACTTTATGGTAAGGAGTTTATTGCAATCTATGGAGCTACCTGAAGAGGAGGGATATACCCATGGTGGTGGTTGTTGCTGCTGTGGAGGGCATGATTAG", "aa": "MKMQALEISERSYQEFTKLKKIIEAMAGETVSDDQVFDFMVRSLLQSMELPEEEGYTHGGGCCCCGGHD", "aa_hexdigest": "d7beb2a870722ff5c03dea976d3b5150", "start_type": "ATG", "rbs_motif": "TTTTT", "ups": { "uniparc_id": "UPI000DC0062F", "uniref100_id": "UniRef100_A0A328EHM2", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DC0062F", "UniRef:UniRef100_A0A328EHM2" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EHM2", "uniref90_id": "UniRef90_A0A660MRV6" }, "psc": { "uniref90_id": "UniRef90_A0A660MRV6", "uniref50_id": "UniRef50_A0A2W4IKK4" }, "pscc": { "uniref50_id": "UniRef50_A0A2W4IKK4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4IKK4" ], "product": "CopG family transcriptional regulator" }, "genes": [ "copG" ], "id": "NKOCPLAKPK_1292", "locus": "GCA003260355_01256" }, { "type": "cds", "sequence": "contig_1", "start": 683746, "stop": 684006, "strand": "+", "frame": 1, "gene": null, "product": "ASCH domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MRA5", "UniRef:UniRef90_A0A660MRA5" ], "nt": "ATGAAAGCACAAATCCTTACACTGAGGACCTGTAAAGAAGGGTTTCAGGACCTCTTGGAACATGGATGCCAGTATAAATACAGAGAAGCAAAGCCTTTTTGGAGAGCAAGGCTTTTTTCTAATGGGCAAGCAAAGCATTTTGACGAGGTACATATCAAGAATGGCTATCAACCGGATTCTCCGCTCGCAATTTATGAATTTTCTGGGATTGAAGGGCCTGAAGTGGTAGAAGGGGTGCCTTGCTTCAAGATTGTTTTGTGA", "aa": "MKAQILTLRTCKEGFQDLLEHGCQYKYREAKPFWRARLFSNGQAKHFDEVHIKNGYQPDSPLAIYEFSGIEGPEVVEGVPCFKIVL", "aa_hexdigest": "448cbe8e2273819bf7927eeeaf259cdc", "start_type": "ATG", "rbs_motif": "TTTA", "psc": { "uniref90_id": "UniRef90_A0A660MRA5", "query_cov": 1.0, "subject_cov": 0.8865979381443299, "identity": 0.988, "score": 184.0, "evalue": 2.52e-59, "valid": true, "product": "ASCH domain-containing protein", "uniref50_id": "UniRef50_A0A660MRA5" }, "pscc": { "uniref50_id": "UniRef50_A0A660MRA5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MRA5" ], "product": "ASCH domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_1293", "locus": "GCA003260355_01257" }, { "type": "cds", "sequence": "contig_1", "start": 684181, "stop": 684510, "strand": "+", "frame": 1, "gene": null, "product": "Magnesium chelatase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3D0UUV3" ], "nt": "ATGATTGCACGTATCAAAACTTTTAATACGCTCGGGCTTCTGGGGCAGGAAATTACCGTAGAGGCTGACAGTAGCAAAGCTCTCCCCACAATCGAAATCATTGGGCTTCCTGATCTGATGATCAAAGAAGCAAAAGAAAGAATAAGAGCCTGTTTTAGGAATGTGGGAATCGATCTTCCCAAGAGGAAATTCATTCTCAATCTCTCTCCCAGCGATCTCAAAAAGGTGGGAACCGCCTTTGACCTCCCTATGGCAGTTGCCTTACTCTGCCTCATTATGGAAGGTAAGGTTGCACACTATGATAGAATCCAGGAGTTTCTTTTCTTCTGA", "aa": "MIARIKTFNTLGLLGQEITVEADSSKALPTIEIIGLPDLMIKEAKERIRACFRNVGIDLPKRKFILNLSPSDLKKVGTAFDLPMAVALLCLIMEGKVAHYDRIQEFLFF", "aa_hexdigest": "c01f2b61e9b83c3b78270c8325805494", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3D0UUV3", "query_cov": 1.0, "subject_cov": 0.9732142857142857, "identity": 0.642, "score": 144.0, "evalue": 1.5e-42, "valid": false, "product": "Magnesium chelatase", "uniref50_id": "UniRef50_A0A3D0UUV3" }, "pscc": { "uniref50_id": "UniRef50_A0A3D0UUV3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3D0UUV3" ], "product": "Magnesium chelatase" }, "genes": [], "id": "NKOCPLAKPK_1294", "locus": "GCA003260355_01258" }, { "type": "cds", "sequence": "contig_1", "start": 684601, "stop": 685050, "strand": "+", "frame": 1, "gene": null, "product": "Magnesium chelatase ChlI-like catalytic domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A351Y3U7" ], "nt": "GTGCCGGCAGAGAATAGCTATGAACTAGAATATATTTCGGACATTACCATTATTCCGCTCAGCCATTTTCAGCAGATCATTGACTTTCTCCTAAAGGGAACAGGGCTTTCTCACCTTCGTAATGCAAAAAATCTCGACGAGCTCAGCTCACAACAACAAAGCTTGGAATCTGATTTTGCCTATATTAAGGGGCAGCTCGTTGCAAAAAGAGCGCTCAGTATCGCAGCAGCAGGACTGCATAATCTCCTCATGGTCGGGGCTCCCGGTAGTGGAAAGACGCTTTTGAGTAAGGCACTGCCGACGATTCTTCCACCCTTGGATACTTCCGAAATCCTTGAAGTGAGTCAGATTTATTCTGTTGTAGGGAAGCTTAACAAAGACCTTCCTCTCGTGATCCAGAGACCCTTTAGACAAGTGCATCATACCGCTTCTAAGGTTTCTATTATTTGA", "aa": "MPAENSYELEYISDITIIPLSHFQQIIDFLLKGTGLSHLRNAKNLDELSSQQQSLESDFAYIKGQLVAKRALSIAAAGLHNLLMVGAPGSGKTLLSKALPTILPPLDTSEILEVSQIYSVVGKLNKDLPLVIQRPFRQVHHTASKVSII", "aa_hexdigest": "f108c25fc57e153883b1a0b47c622871", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A351Y3U7", "query_cov": 1.0, "subject_cov": 0.8186813186813187, "identity": 0.617, "score": 186.0, "evalue": 8.43e-58, "valid": false, "product": "Magnesium chelatase ChlI-like catalytic domain-containing protein", "uniref50_id": "UniRef50_A0A351Y3U7" }, "pscc": { "uniref50_id": "UniRef50_A0A351Y3U7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A351Y3U7" ], "product": "Magnesium chelatase ChlI-like catalytic domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_1295", "locus": "GCA003260355_01259" }, { "type": "cds", "sequence": "contig_1", "start": 685138, "stop": 685488, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01078.27" ], "nt": "GTGCTCGAAGTGCTTAGGCAACCATTAGAAGATGGGATCATTAATATCTCAAGAGTCAATGGAACCGTGCAGTATCCAGCAAATGTCATGTTTATCGCAGCGATGAACCCTTGTAAATGTGGCTACTACAAAGATCGCGAGAAGACTTGTTCCTGTAGCCTCAATGACATCAAAAGATATCAAAGCAAAATCTCTGGTCCCTTACTAGATAGGATCGATATGATCCTAGAAATCCCGAGGGAGAAGGTCGACTCCTTGCTCACACAGGGGCAGCATCAATCTTCAGCCGAACTCCGCAAACAAGTTATCAAAGCTCGAGAACTCCAGAACGAGCGTTTCAGAGGAAGCTGA", "aa": "MLEVLRQPLEDGIINISRVNGTVQYPANVMFIAAMNPCKCGYYKDREKTCSCSLNDIKRYQSKISGPLLDRIDMILEIPREKVDSLLTQGQHQSSAELRKQVIKARELQNERFRGS", "aa_hexdigest": "b0cc87b6266edf794b12b0824eaf8e08", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01078.27", "name": "Mg_chelatase", "length": 84, "aa_cov": 0.7241379310344828, "hmm_cov": 1.0, "evalue": 6.762916527668631e-34, "score": 113.5223159790039, "start": 1, "stop": 84 } ], "seq_stats": { "molecular_weight": 13301.247899999988, "isoelectric_point": 8.980462074279785 }, "id": "NKOCPLAKPK_1296", "locus": "GCA003260355_01260" }, { "type": "cds", "sequence": "contig_1", "start": 685507, "stop": 685731, "strand": "+", "frame": 1, "gene": null, "product": "Mg chelatase-related protein C-terminal domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3D5YDV2" ], "nt": "ATGACAGCAAAAGATCTTGATTATTTTATTCAGCTTGATAGCGAGAGTAAAGATTTTCTCAGGCAAGCGGCTGAAAAGCTTACGCTTTCTGGGAGAGTGATTCATAGATTACTGAAATTGGGGAGAACTATCGCGGATATGGAAGGGAATCCTGATCTCAAAGTCGGACATCTCATGGAAGCGCTCCATTATAGGAGTAAAACGATGTTCGTTGAAGCGGCATAA", "aa": "MTAKDLDYFIQLDSESKDFLRQAAEKLTLSGRVIHRLLKLGRTIADMEGNPDLKVGHLMEALHYRSKTMFVEAA", "aa_hexdigest": "91f01047d38450efcbe5a16af9d436a5", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3D5YDV2", "query_cov": 0.9864864864864865, "subject_cov": 0.9125, "identity": 0.534, "score": 77.8, "evalue": 2.3e-17, "valid": false, "product": "Mg chelatase-related protein C-terminal domain-containing protein", "uniref50_id": "UniRef50_A0A3D5YDV2" }, "pscc": { "uniref50_id": "UniRef50_A0A3D5YDV2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3D5YDV2" ], "product": "Mg chelatase-related protein C-terminal domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_1297", "locus": "GCA003260355_01261" }, { "type": "cds", "sequence": "contig_1", "start": 685810, "stop": 686280, "strand": "+", "frame": 1, "gene": null, "product": "dUTP diphosphatase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MR07", "UniRef:UniRef90_A0A660MR01" ], "nt": "ATGGAAACTATTATCATTTGTGCTAGTCATGAAAATTTTGTCCCAGAAAGGAAGACTCCGGAGGCGATGTGCTGGGATATTAAAGCAGCAGAAAGCCTGAGTATTGCTCCTGGAGAAGTCAAACTCGTCAAAAGTGGAATAAAAACCTTTATCCCTACCGGACGATGCTGTAAAATTTATGCGAGGTCCTCGCTCCCGATCAAGCAGGGACTCCAACTTGCTAATAGTGTAGGGCTCGTGGATTCTGATTACAGAGGCGAATATTTGATGCAGCTCTATAATTTCACAAACCAGCCTGTAGAAATTGAGCAGGGAGCGAGACTTTGTCAGATCGAATTTTGTCCGCATTATCGAGGCACTCAACAATTTGGGATCTCTGAGATCCCAGCACTTCAGATCAAGATCGATCCTGAACTGTTTACGAACTTTGAAGAGGCTTATCCTTCAGTAAGAGGAGCCGGAGGATTCTGA", "aa": "METIIICASHENFVPERKTPEAMCWDIKAAESLSIAPGEVKLVKSGIKTFIPTGRCCKIYARSSLPIKQGLQLANSVGLVDSDYRGEYLMQLYNFTNQPVEIEQGARLCQIEFCPHYRGTQQFGISEIPALQIKIDPELFTNFEEAYPSVRGAGGF", "aa_hexdigest": "2c7bb4bfb6922a920784c6ca21fb35ac", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A660MR01", "query_cov": 1.0, "subject_cov": 0.9629629629629629, "identity": 0.981, "score": 319.0, "evalue": 2.29e-110, "valid": true, "product": "dUTP diphosphatase", "uniref50_id": "UniRef50_A0A660MR07" }, "pscc": { "uniref50_id": "UniRef50_A0A660MR07", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MR07" ], "product": "dUTP diphosphatase" }, "genes": [], "id": "NKOCPLAKPK_1298", "locus": "GCA003260355_01262" }, { "type": "cds", "sequence": "contig_1", "start": 686449, "stop": 687045, "strand": "+", "frame": 1, "gene": "map", "product": "type I methionyl aminopeptidase", "db_xrefs": [ "EC:3.4.11.18", "GO:0006508", "GO:0070006", "KEGG:K01265", "SO:0001217", "UniRef:UniRef50_A0A288DJB0", "UniRef:UniRef90_A0A328EL47" ], "nt": "ATGAAAAATCTGATCAAAACCCCTGAACAAATTCAAAACATCACAGATTCCTGTGCATACCTTACTGAACTCCTCCATTACCTCAAAGACCATACGAAAGCGGGAATGAGCCTCCTTGAAGTCGAGGAACTCTCCCAGCAATGGTTGGATACAAGGAATCTCAAAGGAGCATTTAAGGGATATGAGGGATTTCCAGCAAATCTTTGCCTCTCTGTAAATGATTGCGTTGTCCATGGGATTCCTGACGAAACGGTATTAAAAAATGGTGATCTCTTGAAGATTGATACGGGGATTTTGTATAAAAAGGGTGTCTCTGATGCTTGTATTTCTATGATTATCGGTGATGCAGGGCTTAATCCGCTCGGAGCAGAACTTATCAAGGTAACTAAAGAAGCGCTTGATCGCGGGATAGCGAGCATTCAGGTTGGCGGTTTCGCTTACGACTATTCCAAAGCAGTATATAATCACGTTACGAATAATGGTTTTGAAATCATAAAAACACTGACAGGACATGGCGTGGGTAACGATGTTCACGAACTGCCTCATTTCTACAACTATCCGAACGCCAAGATGAAAAAAATTCAATGGATTCCGTGA", "aa": "MKNLIKTPEQIQNITDSCAYLTELLHYLKDHTKAGMSLLEVEELSQQWLDTRNLKGAFKGYEGFPANLCLSVNDCVVHGIPDETVLKNGDLLKIDTGILYKKGVSDACISMIIGDAGLNPLGAELIKVTKEALDRGIASIQVGGFAYDYSKAVYNHVTNNGFEIIKTLTGHGVGNDVHELPHFYNYPNAKMKKIQWIP", "aa_hexdigest": "70bdaaebcc1b46841b37e1b4efd2332b", "start_type": "ATG", "rbs_motif": "TTTTT", "genes": [ "map" ], "truncated": "3-prime", "pseudogene": { "start": 686449, "stop": 687222, "observations": { "mutation": [ 687043 ], "stop": [ 687043 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EL47", "query_cov": 1.0, "subject_cov": 0.7704280155642024, "identity": 0.995, "score": 409.0, "evalue": 5.48e-144, "gene_start": 1, "gene_end": 198, "reference_start": 1, "reference_end": 198, "reference_sequence": "MKNLIKTPEQIQNITDSCAYLTELLHYLKDHTKAGMSLLEVEELSQQWLDTRNLKGAFKGYEGFPANLCLSVNDCVVHGIPDETVLKNGDLLKIDTGILYKKGVSDACISMIIGDAGLNPLGAELIKVTKEALDRGIASIQVGGFAYDYSKAVYNHVTNNGFEIIKTLTGHGVGNDVHELPHFYNYPNTKMKKIQWIPGMVVALEPITAVKSDDVVYHESDRNLYCRKGDLGAQWEYTVLITEHGPKILAGVVEDLY" }, "paralog": false, "identity": 0.9922178988326849, "score": 523.0, "evalue": 5.6e-191, "description": "Internal stop codon at 687043. Nonsense mutation around 687043.", "psc": { "uniref90_id": "UniRef90_A0A328EL47", "gene": "map", "product": "type I methionyl aminopeptidase", "ec_ids": [ "3.4.11.18" ], "uniref50_id": "UniRef50_A0A288DJB0", "kegg_orthology_id": "K01265", "go_ids": [ "GO:0006508", "GO:0070006" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EL47", "EC:3.4.11.18", "UniRef:UniRef50_A0A288DJB0", "KEGG:K01265", "GO:0006508", "GO:0070006" ] }, "pscc": { "uniref50_id": "UniRef50_A0A288DJB0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A288DJB0" ], "product": "Methionine aminopeptidase" } }, "id": "NKOCPLAKPK_1299", "locus": "GCA003260355_01263" }, { "type": "cds", "sequence": "contig_1", "start": 687046, "stop": 687222, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTGGTTGCTTTGGAACCGATCACTGCGGTAAAGTCCGATGATGTAGTCTATCATGAAAGTGATCGAAATCTTTACTGTAGAAAAGGTGATTTGGGAGCACAATGGGAATATACCGTGCTGATTACCGAGCATGGTCCTAAAATCCTCGCAGGAGTTGTTGAGGACTTGTATTAA", "aa": "MVVALEPITAVKSDDVVYHESDRNLYCRKGDLGAQWEYTVLITEHGPKILAGVVEDLY", "aa_hexdigest": "a25ca2a43a1a38e3975d9788caa7f185", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6521.342500000001, "isoelectric_point": 4.633480644226075 }, "id": "NKOCPLAKPK_1300", "locus": "GCA003260355_01264" }, { "type": "cds", "sequence": "contig_1", "start": 687590, "stop": 687697, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAAGAATTTTCGTCTGCATTGCGTCGTATAGAGACAAACAGTTGCCGAAGACCGTGGCATCGTTATTGGAAAATGCTGAGCATCCTGAGTTGATTGATATTTGA", "aa": "MERIFVCIASYRDKQLPKTVASLLENAEHPELIDI", "aa_hexdigest": "0383ee9206bda10f0051aba1a6c9d129", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4043.664499999999, "isoelectric_point": 5.004696083068848 }, "id": "NKOCPLAKPK_1301", "locus": "GCA003260355_01265" }, { "type": "cds", "sequence": "contig_1", "start": 687701, "stop": 688426, "strand": "+", "frame": 2, "gene": null, "product": "Glycosyltransferase 2-like domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MPG2", "UniRef:UniRef90_A0A328EHG1" ], "nt": "TTGAACCAAGTAGATTTTGAGCAGGATCAGGCTTGTATGCTTGCTGCTCCAGAGGATTATCCTCAAGTGGAGGAACGCTGCATTGATCATACCTTATCAAGAGGATGTTGCTGGGCAAGAGCCTATGTTTACGAGCATCTTTTTAAAGATCAGAAGTTTGTTTTACAATTAGATAGCCATTGTAGGCTGCTAAAAAATTGGGACACTGAAGTCAAAGCAATGCGAAAAGGGCTTGAGGACTCTAAAGCCATTCTCTCACACTATGCTATGCCTTTTGGAGATGATGAGGTTTTGAAAGATGAATATTTTATCAGTTTTGAGTGCCCGCGATTCGATGAGGCTTATAAACTGCCAAGAATCCATCCTCATTCTTCTTGGGAGAGACCTGAGCAACCAAAGCAGATAGCGTTTGTGGGAGGTGGATTTATTTTTGGACTCGGAAAAGCCTTTAGAGAGGTTCCTTATGATCCCTTCCTCTACTTTAATGGAGAAGAGCAGACCTATGCCGCACGTTTATGGACGCATGGCTACAACATCTATGCTCCTCAAAAATCTATTGCTCGGCACTACTACGCAGACAATCCAGATCTCAAAAGAGTGAGACATCGAGAAGACAATAAGCGTTCAGATAATTTAACAGCGTTATCTATTGCTCGTCATAAGCATCTTTTGGGAATAGAAAACAGTGACAATGATGCTGTGATTAGATTCCTCAATCGTTATTGA", "aa": "MNQVDFEQDQACMLAAPEDYPQVEERCIDHTLSRGCCWARAYVYEHLFKDQKFVLQLDSHCRLLKNWDTEVKAMRKGLEDSKAILSHYAMPFGDDEVLKDEYFISFECPRFDEAYKLPRIHPHSSWERPEQPKQIAFVGGGFIFGLGKAFREVPYDPFLYFNGEEQTYAARLWTHGYNIYAPQKSIARHYYADNPDLKRVRHREDNKRSDNLTALSIARHKHLLGIENSDNDAVIRFLNRY", "aa_hexdigest": "38a7711b877252aaac6f5342d6a235f6", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 687590, "stop": 688552, "observations": { "mutation": [ 687695, 688424, 688469 ], "stop": [ 687695, 688424, 688469 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EHG1", "query_cov": 1.0, "subject_cov": 0.753125, "identity": 0.996, "score": 506.0, "evalue": 1.22e-180, "gene_start": 1, "gene_end": 241, "reference_start": 38, "reference_end": 278, "reference_sequence": "MERIFVCIASYRDKQLPKTVASLLENAEHPELIDIGILNQVDFEQDQACMLAAPEDYPQVEERCIDHTLSRGCCWARAYVYEHLFKDQKFVLQLDSHCRLLKNWDTEVKAMRKGLEDSKAILSHYAMPFGDDEVLKDEYFISFECPRFDEAYKLPRIHPHSSWERPEQPKQIAFVGGGFIFGLGKAFREVPYDPFLYFNGEEQTYAARLWTHGYNIYAPQKSIARHYYADNPDLKRVRHREDNKRSDNLTALSIARHKHLLGIENSDNDAVIRFLNRYGLGKERSLKDYEKFSGVCFKEQTVEEFAKKGQVNEEYASAVA" }, "paralog": false, "identity": 0.9875, "score": 655.0, "evalue": 1.26e-241, "description": "Internal stop codon at 687695, 688424, 688469. Nonsense mutation around 687695, 688424, 688469.", "psc": { "uniref90_id": "UniRef90_A0A328EHG1", "product": "Glycosyltransferase 2-like domain-containing protein", "uniref50_id": "UniRef50_A0A660MPG2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EHG1", "UniRef:UniRef50_A0A660MPG2" ] }, "pscc": { "uniref50_id": "UniRef50_A0A660MPG2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MPG2" ], "product": "Glycosyltransferase" } }, "id": "NKOCPLAKPK_1302", "locus": "GCA003260355_01266" }, { "type": "cds", "sequence": "contig_1", "start": 688825, "stop": 689352, "strand": "+", "frame": 1, "gene": null, "product": "exodeoxyribonuclease III", "db_xrefs": [ "EC:3.1.11.2", "GO:0004518", "GO:0006281", "KEGG:K01142", "SO:0001217", "UniRef:UniRef50_A0A5P8NE65", "UniRef:UniRef90_A0A660MQ98" ], "nt": "ATGAAAATTATTTCTCGAAATGTTAATGGGATTAGAGCTGTACTTCAAAAGGGTTTTCTTGATTTTGTAAAGCAAGAGAATCCTGATGTACTTTGTCTGCAGGAAGTGAAAGCATTTGAGCATCAGTTACCAACAGAAATCAGATTTGCAATGCAGGACTATGACTATATTTGGCATCGTAGCGAAAGACCCGGCTATGCGGGGACAGCAATCTTTTATAAGAAGTCACTTCCAATCTTAGCAAAGCATAGTGCTTTTGAAAAACCTGGATGTTTTAATGATGATGGGAGAGTGACTGCGGTTGAATGGTCAGATTTTTTACTGTTGAATATTTACTTTCCTAATGGTGGAGATCGTGCTGATGGAACTGAAATGCTGAGTTATAAGCTGGATTTTTACAAGCAATATCAGGAGTTTGTGCAGCAGAAGGTAAAAGAGGGGAAGAAAGTCATCACCACTGGAGATTTCAATATCTGCCATCGTCCAATTGATATTGCAAGACCGAAGGAAAATGAAAACTCGATCTGA", "aa": "MKIISRNVNGIRAVLQKGFLDFVKQENPDVLCLQEVKAFEHQLPTEIRFAMQDYDYIWHRSERPGYAGTAIFYKKSLPILAKHSAFEKPGCFNDDGRVTAVEWSDFLLLNIYFPNGGDRADGTEMLSYKLDFYKQYQEFVQQKVKEGKKVITTGDFNICHRPIDIARPKENENSI", "aa_hexdigest": "bc3c8521caab0b998783fe928172f052", "start_type": "ATG", "rbs_motif": "TTATT", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 688825, "stop": 689616, "observations": { "mutation": [ 689350, 689398 ], "stop": [ 689350, 689398 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A660MQ98", "query_cov": 1.0, "subject_cov": 0.6653992395437263, "identity": 0.983, "score": 365.0, "evalue": 1.25e-126, "gene_start": 1, "gene_end": 175, "reference_start": 1, "reference_end": 175, "reference_sequence": "MKIISRNVNGIRAVLQKGFLDFVKQENPDILCLQEVKAFEHQLPIEIRFAMQDYDYIWHRSERPGYAGTAIFYKKSLPILSKHSAFEKPGCFNDDGRVTAVEWSDFLLLNIYFPNGGDRADGTEMLSYKLDFYKQYQEFVQQKVKEGKKVITTGDFNICHRPIDIARPKENENSIGFLPIERAEMDAMEASGLIDVFRLQNPELGEKYTWRSYRAGARPRNVGWRLDYFRVSENMRAQLGTMMHYDQVMGSDHCPIALFIQYE" }, "paralog": false, "identity": 0.9771863117870723, "score": 533.0, "evalue": 1.57e-195, "description": "Internal stop codon at 689350, 689398. Nonsense mutation around 689350, 689398.", "psc": { "uniref90_id": "UniRef90_A0A660MQ98", "product": "exodeoxyribonuclease III", "ec_ids": [ "3.1.11.2" ], "uniref50_id": "UniRef50_A0A5P8NE65", "kegg_orthology_id": "K01142", "go_ids": [ "GO:0004518", "GO:0006281" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A660MQ98", "EC:3.1.11.2", "UniRef:UniRef50_A0A5P8NE65", "KEGG:K01142", "GO:0004518", "GO:0006281" ] }, "pscc": { "uniref50_id": "UniRef50_A0A5P8NE65", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A5P8NE65" ], "product": "Exodeoxyribonuclease III" } }, "id": "NKOCPLAKPK_1303", "locus": "GCA003260355_01267" }, { "type": "cds", "sequence": "contig_1", "start": 689527, "stop": 689616, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGAGCTCAACTTGGGACTATGATGCATTATGATCAGGTAATGGGTTCTGATCACTGCCCTATTGTGCTCTTCATCCAATACGAATAA", "aa": "MRAQLGTMMHYDQVMGSDHCPIVLFIQYE", "aa_hexdigest": "7ec5be216772f11fa62b6ee1d106feea", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3414.9723999999997, "isoelectric_point": 5.353857994079591 }, "id": "NKOCPLAKPK_1304", "locus": "GCA003260355_01268" }, { "type": "cds", "sequence": "contig_1", "start": 689678, "stop": 690400, "strand": "-", "frame": 3, "gene": null, "product": "Thymidylate synthase/dCMP hydroxymethylase domain-containing protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBF469E", "UniRef:UniRef100_A0A328EH91", "UniRef:UniRef50_A0A328EH91", "UniRef:UniRef90_A0A328EH91" ], "nt": "ATGAACGAATATCTAGAAGAGTATTTAGCATTCCTGCGTTCTATCGTAAATGATGGGAAACCAGGAAAAAATCCTGAGTGTATATCTTTGTGGGTCTACAAGTTGCAAATCAAGCTAAACCAGCAAGAGAATAGGGAAATCTCCAACTTTCCTTTGCTTAGCTCTCCAAAGGTGGATTTTGAAGCCGTTTGTAAAGCGGTGCTCTCCATGATAGAGAAAGCACTCACTTCTGATTCGGCGCAAAACCCAGACGAAAGTGAAGCTTCAATGCGTCAAAAAACGCTTCGAAAGGTAATTGATTTGATCATTAATAAGCCTGAGGATTGCTCTATGATCTTCAACGATCCAGAGTCAACAGGTGCTAGTTACCATTTTACAGTGAGTAATGGCACTCTTAACCTAGTCGTGAGGCGTGAAGGTGATGATAATGATGTGATTGATGCAGTTCCCAGTGAAATTGCCAAAGCTGCGCTTTTCTTGTGTCTGATGGCACATGTCACGGGAAGGGTGCCAGGAACTATTTACTTCTTCTTTGTTGATCCCCATATCAGGAAGCAAGAATATGAGCGATTGAAGAAGCAATTCGGAGGAACTTCAGTAGCTGGTTCTGAGCCAGGACCGTCTCCTCGTCTTTGTCTCAATCCCGAGAAAAAAGCGATAGGAGATATTAAACGGTCTGATATCCAGCTCTTCGGCTCGAGTCCTCAAGTCAGAGGATCATAG", "aa": "MNEYLEEYLAFLRSIVNDGKPGKNPECISLWVYKLQIKLNQQENREISNFPLLSSPKVDFEAVCKAVLSMIEKALTSDSAQNPDESEASMRQKTLRKVIDLIINKPEDCSMIFNDPESTGASYHFTVSNGTLNLVVRREGDDNDVIDAVPSEIAKAALFLCLMAHVTGRVPGTIYFFFVDPHIRKQEYERLKKQFGGTSVAGSEPGPSPRLCLNPEKKAIGDIKRSDIQLFGSSPQVRGS", "aa_hexdigest": "ab2706e69c9ff38beef26977c1d70566", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000DBF469E", "uniref100_id": "UniRef100_A0A328EH91", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBF469E", "UniRef:UniRef100_A0A328EH91" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EH91", "uniref90_id": "UniRef90_A0A328EH91" }, "psc": { "uniref90_id": "UniRef90_A0A328EH91", "product": "Thymidylate synthase/dCMP hydroxymethylase domain-containing protein", "uniref50_id": "UniRef50_A0A328EH91" }, "pscc": { "uniref50_id": "UniRef50_A0A328EH91", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EH91" ], "product": "Thymidylate synthase/dCMP hydroxymethylase domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_1305", "locus": "GCA003260355_01269" }, { "type": "cds", "sequence": "contig_1", "start": 690412, "stop": 690948, "strand": "-", "frame": 1, "gene": null, "product": "DHFR domain-containing protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBF72D0", "UniRef:UniRef100_A0A328EGU2", "UniRef:UniRef50_A0A328EGU2", "UniRef:UniRef90_A0A328EGU2" ], "nt": "ATGAGAACAAAAATAATAATGATTGTTTCCATTTCGAAAAATGGAGTAATCGCAGGAATGGGAAAGGGGTACCTGACTCCGTGGCCCATGAGAAATGATGAAGAGAGTTTTAAAGAGACTGTTAACGGGAAATTGGTTGTTGCAGATGAGCAGGTATGGAGATTATTGACCCACCGAAAATTGCTCGAGAATTGCAAGAGCCTGGCCTTTAGCCTTGGCAAAAGAATACACTTGCCACAATCAGTCCCTGTAGCAAAAAGTGTTGAGGAGGTAATATCCCATGCAGAGAAAACATCGCAAGATATTGTAGTTGTAGGAGGAATGGAGGTTTATCAAGCTTTCATGCCTTCAGTATCGAAAGTATGTATAAGCCGGATTTGTGGTCTGGTTGAACGTAGTACCGATTCTGTATATTTCGATGAGGAATTGCACAATTCCATTCGAGAGAATTTCGATAAAATCTTCGATGACGTGATGGAACGAGATGAGCAAAATCCCTATCCTATAGCCTATTCTATCTACAAAAGAATAAACTAA", "aa": "MRTKIIMIVSISKNGVIAGMGKGYLTPWPMRNDEESFKETVNGKLVVADEQVWRLLTHRKLLENCKSLAFSLGKRIHLPQSVPVAKSVEEVISHAEKTSQDIVVVGGMEVYQAFMPSVSKVCISRICGLVERSTDSVYFDEELHNSIRENFDKIFDDVMERDEQNPYPIAYSIYKRIN", "aa_hexdigest": "79b536107eac077874903d745ae17853", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000DBF72D0", "uniref100_id": "UniRef100_A0A328EGU2", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBF72D0", "UniRef:UniRef100_A0A328EGU2" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EGU2", "uniref90_id": "UniRef90_A0A328EGU2" }, "psc": { "uniref90_id": "UniRef90_A0A328EGU2", "product": "DHFR domain-containing protein", "uniref50_id": "UniRef50_A0A328EGU2" }, "pscc": { "uniref50_id": "UniRef50_A0A328EGU2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EGU2" ], "product": "DHFR domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_1306", "locus": "GCA003260355_01270" }, { "type": "cds", "sequence": "contig_1", "start": 691044, "stop": 691286, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTCCTTGCTCATCCAGATATTGTAGTCATGGACGAGCCGACCAACCATCTAGATTTGACTTCCAAAGAGGCGATCAAAACGATGCTCGCAGATTTCAATGGGGTTTCCTTGATCGTTTCGCATGATAGAGATTTTTTGGAAGCAACTTCAGAGCTTCTCTGGGTGATCAAAGAGGGGAAATTGACCGTTTTTCATTCCTTTGAGAGAGGATTTGATGAGATGAGAAGCAGGAATATCTGA", "aa": "MLLAHPDIVVMDEPTNHLDLTSKEAIKTMLADFNGVSLIVSHDRDFLEATSELLWVIKEGKLTVFHSFERGFDEMRSRNI", "aa_hexdigest": "1c56991dd98d0d4c11625cdc507ccbed", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9172.414199999994, "isoelectric_point": 4.978777503967284 }, "id": "NKOCPLAKPK_1307", "locus": "GCA003260355_01271" }, { "type": "cds", "sequence": "contig_1", "start": 691581, "stop": 692141, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF12848.13" ], "nt": "TTGCAAATAGCAAAATTTCTGCTTCAAGATGTGGATTTGCTGATTTTGGATGAGCCGACCAACCATCTGGATATTGAGGGGATTCTCTTTATTGAGCAGTTCTGTCAGATGCGAAACAAGGCGCTGATCTGCATTTCTCACGATAGAAAATTTCTGGCTTCTGCATTCACCAAAACGATAGAAATCAAAAACAAAAAGCTGAATCTTTACTATTGTGGCTATGAAGACTTCTTGAGAGAAAAAGAAAAAAGGGCTGAACTCCAGCTCAAGAACTATACTGCACAGCAGAAATACCTTGCTCAGCAGGAGAAATTCATTGAGAGATTCCGCTACAAATCTAGTAAAGCCGCCCAGGTGCAAAGTAGAATCAAAATGCTAGACAAGATGGATCGTATTGAGGCTCCAGAAGAGGAAATTTCAGCACATACTCCAAATCTTCAGGTCAAATGGAGACTTCCAGAGACCTTGATCAGACTTTCTGAACTTTCTGTCGGATATGGGCAAAGCGTGCTAGTTAATCTGCCTCGTGAACTGGAAATTACCAAAGCGATGAAAATCTGA", "aa": "MQIAKFLLQDVDLLILDEPTNHLDIEGILFIEQFCQMRNKALICISHDRKFLASAFTKTIEIKNKKLNLYYCGYEDFLREKEKRAELQLKNYTAQQKYLAQQEKFIERFRYKSSKAAQVQSRIKMLDKMDRIEAPEEEISAHTPNLQVKWRLPETLIRLSELSVGYGQSVLVNLPRELEITKAMKI", "aa_hexdigest": "2dee687ce15311595b1f3d460e61597e", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF12848.13", "name": "ABC_tran_Xtn", "length": 79, "aa_cov": 0.42473118279569894, "hmm_cov": 1.0, "evalue": 1.7904280633735446e-27, "score": 91.74992370605469, "start": 60, "stop": 138 } ], "seq_stats": { "molecular_weight": 21942.349000000013, "isoelectric_point": 8.858874702453612 }, "id": "NKOCPLAKPK_1308", "locus": "GCA003260355_01272" }, { "type": "cds", "sequence": "contig_1", "start": 692445, "stop": 692918, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00005.33" ], "nt": "ATGCAACTTTCCGAAATCATTGAATCTGTCCAATCGCTTCATCCAGATTTTTCTTATGAAGAAATTGCTGAACTTTGCTATGATTACCTCGCTCAATTCAACGATAAAACCAAAGAATCCACCAAAGAAAAAAGTCTGAAAAAATACCTCAGTAAAGAATTTGGAATTCAGCGAGAAACCGAAAATCCAGGAGAAGACTGTGAAAAGCAAGTCTCGTTTTCTAGCATTCCAAGACATCTTTTCAACTATACGAACAAAAAGAACGCTGACTACCTCATCACGATCAAGGGCTTGAGCAAACAACTCGGCTCTACGATTCTCTTTGAAGATGCAGAACTGAAGATCAAGCCTGAAGACAAGATTGCTCTCGTGGGAAAAAATGGAGCTGGGAAGTCCACTTTCCTCAAAATCCTGCTTAATCCTGAACTCGCAGATCATGGCGAGATTGAACTCCTAAAAGATACAAAAATCTGA", "aa": "MQLSEIIESVQSLHPDFSYEEIAELCYDYLAQFNDKTKESTKEKSLKKYLSKEFGIQRETENPGEDCEKQVSFSSIPRHLFNYTNKKNADYLITIKGLSKQLGSTILFEDAELKIKPEDKIALVGKNGAGKSTFLKILLNPELADHGEIELLKDTKI", "aa_hexdigest": "d41f99ddfad769cfd6cc6b141fcfd120", "start_type": "ATG", "rbs_motif": "TTATT", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00005.33", "name": "ABC_tran", "length": 44, "aa_cov": 0.2802547770700637, "hmm_cov": 1.0, "evalue": 1.474677128253474e-10, "score": 38.29771423339844, "start": 109, "stop": 152 } ], "seq_stats": { "molecular_weight": 17878.124199999995, "isoelectric_point": 5.328564643859862 }, "id": "NKOCPLAKPK_1309", "locus": "GCA003260355_01273" }, { "type": "cds", "sequence": "contig_1", "start": 693051, "stop": 693311, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000C7A2A5C" ], "nt": "ATGAAGATGAACAAGTTACTCACGACGACACTTAGCCTCGGGGCAATCCTTTTATTAGCAGGATGTGGGACTCAAAATTCAGGTCCGACGATACAACTCGTAAATCCAGATAGTTGGAAAACAATGATTCCTGAAACTTGTCAAAGCTTTTTTGATGGATGTAACAACTGTACGAGAACTGCTGATGGAGAGGCTGCCTGCACGGAAATGTTCTGTGAAAGATATGAAATGCCAAAATGCCTTGATGATGAGCAAGGATGA", "aa": "MKMNKLLTTTLSLGAILLLAGCGTQNSGPTIQLVNPDSWKTMIPETCQSFFDGCNNCTRTADGEAACTEMFCERYEMPKCLDDEQG", "aa_hexdigest": "c43067a61aa5b4d4d823d0ea4b610cc7", "start_type": "ATG", "rbs_motif": "TTTTTA", "psc": { "uniref90_id": "UniRef90_UPI000C7A2A5C", "query_cov": 0.8604651162790697, "subject_cov": 0.8604651162790697, "identity": 0.5, "score": 80.1, "evalue": 5.27e-18, "valid": false, "uniref50_id": "UniRef50_UPI000C7A2A5C" }, "pscc": { "uniref50_id": "UniRef50_UPI000C7A2A5C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000C7A2A5C" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9404.691300000002, "isoelectric_point": 4.373726463317872 }, "id": "NKOCPLAKPK_1310", "locus": "GCA003260355_01274" }, { "type": "cds", "sequence": "contig_1", "start": 693417, "stop": 693668, "strand": "+", "frame": 3, "gene": null, "product": "DUF333 domain-containing protein", "db_xrefs": [ "KEGG:K09712", "SO:0001217", "UniRef:UniRef50_A0A328EHS5", "UniRef:UniRef90_A0A328EHS5" ], "nt": "ATGATTGATGGAAAGGAAGTTGATGAATGGGAGTACTATAGAGCTGGAATGGAAAAAGCAAGTGAGGAAAGTGCGAAAGCTTATATTGGACGCTCTGCCGAGGAAGTACAAAAAGAATTTGAAGAAAAAAATCAAATCGTTCGTATCGTTTTACAGGACGGACAAGCCATGGCTTTAACTGCAGACTACAGACCTGACCGCTTAAATATTGCCGTGGAGAAGGGAGTAATTACTAATGCTTACTTTGGATAA", "aa": "MIDGKEVDEWEYYRAGMEKASEESAKAYIGRSAEEVQKEFEEKNQIVRIVLQDGQAMALTADYRPDRLNIAVEKGVITNAYFG", "aa_hexdigest": "de11445e0d97187e6f2d5af9d590bea7", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 693258, "stop": 693668, "observations": { "mutation": [ 693309, 693396 ], "stop": [ 693309, 693396 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EHS5", "query_cov": 1.0, "subject_cov": 0.6102941176470589, "identity": 1.0, "score": 160.0, "evalue": 3.07e-49, "gene_start": 1, "gene_end": 83, "reference_start": 54, "reference_end": 136, "reference_sequence": "MFCERYEMPKCLDDEQGGDQKVGMANPASEYCVAQGGESKIKKDENGSEYGVCMIDGKEVDEWEYYRAGMEKASEESAKAYIGRSAEEVQKEFEEKNQIVRIVLQDGQAMALTADYRPDRLNIAVEKGVITNAYFG" }, "paralog": false, "identity": 0.9852941176470589, "score": 272.0, "evalue": 1.14e-95, "description": "Internal stop codon at 693309, 693396. Nonsense mutation around 693309, 693396.", "psc": { "uniref90_id": "UniRef90_A0A328EHS5", "product": "DUF333 domain-containing protein", "uniref50_id": "UniRef50_A0A328EHS5", "kegg_orthology_id": "K09712", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EHS5", "UniRef:UniRef50_A0A328EHS5", "KEGG:K09712" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EHS5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EHS5" ], "product": "DUF333 domain-containing protein" } }, "id": "NKOCPLAKPK_1311", "locus": "GCA003260355_01275" }, { "type": "tRNA", "sequence": "contig_1", "start": 693780, "stop": 693852, "strand": "-", "gene": "trnT", "product": "tRNA-Thr(cgt)", "amino_acid": "Thr", "anti_codon": "cgt", "score": 73.7, "nt": "GCCACAATAGCTCAATTGGCAGAGCACCGCTATCGTAAAGCGGGGGTTAAGGGTTCGATTCCCTTTTGTGGCT", "db_xrefs": [ "SO:0000270" ], "anti_codon_pos": [ 693817, 693819 ], "id": "NKOCPLAKPK_28", "locus": "GCA003260355_01276" }, { "type": "cds", "sequence": "contig_1", "start": 694121, "stop": 694291, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EHN2", "UniRef:UniRef90_A0A328EHN2" ], "nt": "ATGTTTTCTACATTAGCTTATGAGTATGAGAAACTTCATCCAGTAGAGCAAAAGGATTTTAAAAAGGAGTTTAAGGCACTTTTGAAAATGTCACGAGAGCGTAGTGTTGCTCAAAAAGCTTGGAAAGACTTCTTAGAAGATTGGCAAGAGTTGTTGCCATTGACTTTTTGA", "aa": "MFSTLAYEYEKLHPVEQKDFKKEFKALLKMSRERSVAQKAWKDFLEDWQELLPLTF", "aa_hexdigest": "aa99a6cd54de624eacadcfb215060ded", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 693962, "stop": 694378, "observations": { "mutation": [ 693965, 694094, 694289 ], "stop": [ 693965, 694094, 694289 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EHN2", "query_cov": 1.0, "subject_cov": 0.4057971014492754, "identity": 1.0, "score": 114.0, "evalue": 2.74e-31, "gene_start": 1, "gene_end": 56, "reference_start": 54, "reference_end": 109, "reference_sequence": "MGIENIQSTPREKSSIPPILKALKAYRPTILSALSLPPFKDGATGKRNVYETEMFSTLAYEYEKLHPVEQKDFKKEFKALLKMSRERSVAQKAWKDFLEDWQELLPLTFGYQSNDYQQHYLPRKNSLQAFYDTYLNAA" }, "paralog": false, "identity": 0.9782608695652174, "score": 270.0, "evalue": 1.84e-95, "description": "Internal stop codon at 693965, 694094, 694289. Nonsense mutation around 693965, 694094, 694289.", "psc": { "uniref90_id": "UniRef90_A0A328EHN2", "uniref50_id": "UniRef50_A0A328EHN2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EHN2", "UniRef:UniRef50_A0A328EHN2" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EHN2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EHN2" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 6868.883900000001, "isoelectric_point": 6.562425422668457 }, "id": "NKOCPLAKPK_1312", "locus": "GCA003260355_01277" }, { "type": "cds", "sequence": "contig_1", "start": 694465, "stop": 695136, "strand": "+", "frame": 1, "gene": null, "product": "ribulose-bisphosphate carboxylase", "db_xrefs": [ "EC:4.1.1.39", "KEGG:K01601", "SO:0001217", "UniRef:UniRef50_Q12TQ0", "UniRef:UniRef90_A0A7W4ECB6" ], "nt": "ATGACCAATACAAAAGAACTCTTAGCATCTTTGAATGCGCACCAACTCGCTTATGTAAACCTCGATCTTCCAAACCCAAAGAATGGACAGTATATGCTCTGTGTCTTCCGCTTAGAGCCAGGAAAGAAATTGAATATGCTCCAAGCAGCCTGTGAAGTCGCTGCAGAATCTTCTACCGGAACGAATTTCCTCGTGGAAACTGAAACTGCATTTTCAAAGGAAATGAATGCTCTTGTGTATAAACTCGACGAGGAGAAAGAACTCGTATGGATTGCCTATCCGTGGAGGCTCTTTGATAGAGGGGGAAATGTTCAGAATATTCTGACCTATGTCGCAGGGAATGTCTTTGGAATGAAGGAAGTAAAGGCTTTGAAACTGCTTGATGTATGGTTTCCACCTGCAATGCTTGAGCAGTATGATGGACCAAGCTATACCCTAGCAGATATGAGGAATTACCTAGGAGTCTACAATAGACCGATCCTTGGAACAATTATTAAGCCGAAAATGGGACTTACCTCTGCTGAATATGCCGAAGTCTGCTATGATTTCTGGGTAGGTGGAGGAGATTTCGTGAAGAATGACGAACCGCAGGCGAATCAGGATTTTTGTCCTTATGATAAAATGGTGAAACATGTAAAAGAAGCCATGGACAAAGCAGTAAAAGAAACCTGA", "aa": "MTNTKELLASLNAHQLAYVNLDLPNPKNGQYMLCVFRLEPGKKLNMLQAACEVAAESSTGTNFLVETETAFSKEMNALVYKLDEEKELVWIAYPWRLFDRGGNVQNILTYVAGNVFGMKEVKALKLLDVWFPPAMLEQYDGPSYTLADMRNYLGVYNRPILGTIIKPKMGLTSAEYAEVCYDFWVGGGDFVKNDEPQANQDFCPYDKMVKHVKEAMDKAVKET", "aa_hexdigest": "8f7c070ca54c4d0512af378576c6eb17", "start_type": "ATG", "rbs_motif": "TTCTT", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 694465, "stop": 695439, "observations": { "mutation": [ 695134 ], "stop": [ 695134 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A7W4ECB6", "query_cov": 1.0, "subject_cov": 0.4588477366255144, "identity": 0.9329999999999999, "score": 435.0, "evalue": 3.77e-150, "gene_start": 1, "gene_end": 223, "reference_start": 1, "reference_end": 223, "reference_sequence": "MTNTNELVKTLNSHQLAYINLDLPNPKNGQYMLTAFHLKPGKKLNLLQAACEVAAESSTGTNFLVETETAFSKEMNALVYKIDEEKELVWIAYPWRLFDRGGNVQNILTYIAGNVFGMKEVKALKLLDVWFPPAMLEQYDGPSYTLADMRNYLNVHNRPILGTIIKPKMGLTSAEYAEVCYDFWVGGGDFVKNDEPQANQDFCPYDKMVKHVKEAMDKAVKETGQKKVHSFNVSAADYDTMIERCEMIVNAGFEKGSYAFLIDGTTAGWMAVQTLRRKYPDVFIHFHRAGHGAFTRPENPIGYSVLVLSKFARLAGASGIHTGTAGVGKMQGDPAEDITSAENIRYMKKTGHIFEQSWGTIPETDKDFLQIVQRDEDNEEVLRDDSWRAVKTCCPIISGGLNPTLLKPFIDLMGNVDFITTMGAGCHAHPKGTQAGARALVQACEAYQKGIDIHEYAKNKPELAEAIAFFEKPETKEKMSTLRIAA" }, "paralog": false, "identity": 0.9444444444444444, "score": 636.0, "evalue": 8.2e-232, "description": "Internal stop codon at 695134. Nonsense mutation around 695134.", "psc": { "uniref90_id": "UniRef90_A0A7W4ECB6", "product": "ribulose-bisphosphate carboxylase", "ec_ids": [ "4.1.1.39" ], "uniref50_id": "UniRef50_Q12TQ0", "kegg_orthology_id": "K01601", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A7W4ECB6", "EC:4.1.1.39", "UniRef:UniRef50_Q12TQ0", "KEGG:K01601" ] } }, "id": "NKOCPLAKPK_1313", "locus": "GCA003260355_01278" }, { "type": "cds", "sequence": "contig_1", "start": 695146, "stop": 695925, "strand": "+", "frame": 1, "gene": null, "product": "ribulose-bisphosphate carboxylase", "db_xrefs": [ "EC:4.1.1.39", "KEGG:K01601", "SO:0001217", "UniRef:UniRef50_Q12TQ0", "UniRef:UniRef90_A0A7W4ECB6" ], "nt": "GTGCATTCCTTCAATGTTTCAGCGGCGGATTATGATACGATGCTTGAAAGATGTGAAATGATCGTGAATGCAGGATTTGAACCAGGCTCTTATGCTTTCTTGATTGATGGAACGACCGCAGGTTGGATGGCAGTGCAGACCTTGAGAAGAAAATATCCTGATGTCTTCATCCACTTCCATAGAGCTGGACACGGTGCGTTTACCAGACCAGAAAACCCTATTGGATACTCTGTTTTGGTGCTTTCAAAGTTCGCAAGACTCGCTGGAGCCTCTGGAATCCATACCGGAACCGCAGGAGTAGGGAAAATGGCAGGAGATAAAGCCGAAGATATTACTGCTGCAGAAGGAATCAGATATATGAAAAAGTCTGGTCATATCTTTGAGCAGTCTTGGGGAACGATTCCAGAAACGGATAAAGATTTCATTGAACTCGTGCAGAGAGATGAAGATAATGAAGAAGTTTTGAGAGATGATAGCTGGAGAGCAGTAAAAACTTGCTGCCCTATTATTTCTGGAGGACTCAATCCTACGCTTTTGAAGCCGTTTATTGACCTTATGGGAAATGTAGACTTCATCACGACCATGGGAGCTGGTTGTCATGCTCATCCAAAAGGAACGCAGGCAGGAGCTAAAGCTCTCGTTCAGGCTTGTGAGGCTTATCAAAAAGGCATTGATATTCATGAATATGCAAAGAATAAACCAGAACTCACAGAGGCGATTGCATTCTTTGAGAAGCCGGAAACGAAGGAGAAGATGAAAACTTTGAGAATCGCCGCTTAG", "aa": "MHSFNVSAADYDTMLERCEMIVNAGFEPGSYAFLIDGTTAGWMAVQTLRRKYPDVFIHFHRAGHGAFTRPENPIGYSVLVLSKFARLAGASGIHTGTAGVGKMAGDKAEDITAAEGIRYMKKSGHIFEQSWGTIPETDKDFIELVQRDEDNEEVLRDDSWRAVKTCCPIISGGLNPTLLKPFIDLMGNVDFITTMGAGCHAHPKGTQAGAKALVQACEAYQKGIDIHEYAKNKPELTEAIAFFEKPETKEKMKTLRIAA", "aa_hexdigest": "a1d47193b926cd9c4f58f3b5ab1305dd", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 694846, "stop": 695925, "observations": { "mutation": [ 695134 ], "stop": [ 695134 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A7W4ECB6", "query_cov": 1.0, "subject_cov": 0.5329218106995884, "identity": 0.946, "score": 504.0, "evalue": 1.05e-176, "gene_start": 1, "gene_end": 259, "reference_start": 228, "reference_end": 486, "reference_sequence": "MTNTNELVKTLNSHQLAYINLDLPNPKNGQYMLTAFHLKPGKKLNLLQAACEVAAESSTGTNFLVETETAFSKEMNALVYKIDEEKELVWIAYPWRLFDRGGNVQNILTYIAGNVFGMKEVKALKLLDVWFPPAMLEQYDGPSYTLADMRNYLNVHNRPILGTIIKPKMGLTSAEYAEVCYDFWVGGGDFVKNDEPQANQDFCPYDKMVKHVKEAMDKAVKETGQKKVHSFNVSAADYDTMIERCEMIVNAGFEKGSYAFLIDGTTAGWMAVQTLRRKYPDVFIHFHRAGHGAFTRPENPIGYSVLVLSKFARLAGASGIHTGTAGVGKMQGDPAEDITSAENIRYMKKTGHIFEQSWGTIPETDKDFLQIVQRDEDNEEVLRDDSWRAVKTCCPIISGGLNPTLLKPFIDLMGNVDFITTMGAGCHAHPKGTQAGARALVQACEAYQKGIDIHEYAKNKPELAEAIAFFEKPETKEKMSTLRIAA" }, "paralog": false, "identity": 0.9554317548746518, "score": 716.0, "evalue": 1.53e-262, "description": "Internal stop codon at 695134. Nonsense mutation around 695134.", "psc": { "uniref90_id": "UniRef90_A0A7W4ECB6", "product": "ribulose-bisphosphate carboxylase", "ec_ids": [ "4.1.1.39" ], "uniref50_id": "UniRef50_Q12TQ0", "kegg_orthology_id": "K01601", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A7W4ECB6", "EC:4.1.1.39", "UniRef:UniRef50_Q12TQ0", "KEGG:K01601" ] } }, "id": "NKOCPLAKPK_1314", "locus": "GCA003260355_01279" }, { "type": "cds", "sequence": "contig_1", "start": 696329, "stop": 696802, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MUR5", "UniRef:UniRef90_A0A660MUR5" ], "nt": "ATGGGACAGTATTCTACGCTTATCTACCAATCGGTCGACCACACTGCGAGGGCGCTCCTTGCACATTTTCAGGCACAATATGGAAAGCTGATCTTTATCCCACTTGGGATTTCCGCTAAGACACCCGAGAAAATCGCATTAGCCTCTAGTCTGAGCGTTGAAAGGCATTTGCTCCGTAGTTTTGAAGGCGAAGGAAAGCTGATGCTGGTGATTCAAAACGCTGAGATTCGTCAGGTATATAATGATTTTATGGATGGGCTCTATGGATCACTGAGCTTTGTCGGTAAGACTGCGATGAAAGCGTTAGGGATGAGGATGTATCGAGATGATCTTGATGCTCAGCTCAAGAATAATAAGCTCAATAAACCCAGCGTTTATGAGAAGCGAAAAGATAACCTTTTGATTATTTTGAGAGCATTATTTGGGGAGTTGCAGGCAAACCTTCAGCAGGCTGGAGTACAAGTGGTGTTTTGA", "aa": "MGQYSTLIYQSVDHTARALLAHFQAQYGKLIFIPLGISAKTPEKIALASSLSVERHLLRSFEGEGKLMLVIQNAEIRQVYNDFMDGLYGSLSFVGKTAMKALGMRMYRDDLDAQLKNNKLNKPSVYEKRKDNLLIILRALFGELQANLQQAGVQVVF", "aa_hexdigest": "ebee63f6e041a0f1cbc742e1948aaf8b", "start_type": "ATG", "rbs_motif": "TTATT", "psc": { "uniref90_id": "UniRef90_A0A660MUR5", "query_cov": 1.0, "subject_cov": 0.8820224719101124, "identity": 0.981, "score": 298.0, "evalue": 7.11e-102, "valid": true, "uniref50_id": "UniRef50_A0A660MUR5" }, "pscc": { "uniref50_id": "UniRef50_A0A660MUR5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MUR5" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17668.37959999999, "isoelectric_point": 9.453402137756349 }, "id": "NKOCPLAKPK_1315", "locus": "GCA003260355_01280" }, { "type": "cds", "sequence": "contig_1", "start": 697016, "stop": 697156, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGAAAAGAGGAATTAGTCAGATTTTTTCTCTTTGAGATAGCCATTTTTGAAAGAAGTTCGGTCCATGGATTTTTTGCCCTCGGGCTTGAGGGAAAGGGTTTTGATAGCAGGATGAAGGCGATAGCTGGAGTCCCATAG", "aa": "MRKEELVRFFLFEIAIFERSSVHGFFALGLEGKGFDSRMKAIAGVP", "aa_hexdigest": "84665502e00dc035de4e53982796ef1a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5235.0917, "isoelectric_point": 8.275242424011232 }, "id": "NKOCPLAKPK_1316", "locus": "GCA003260355_01281" }, { "type": "cds", "sequence": "contig_1", "start": 697427, "stop": 698251, "strand": "-", "frame": 3, "gene": "yidD", "product": "membrane protein insertion efficiency factor YidD", "db_xrefs": [ "KEGG:K08998", "SO:0001217", "UniRef:UniRef50_A0A7W4HL53", "UniRef:UniRef90_A0A660MXR5" ], "nt": "ATGCTTTCCCATCTGCTTATTTCTACCCTGAAAAAACTAGACCGCTTTCTAGCTCGAATCACTATTGGACTCATCCGTTGCTATCAGGCAACCCTCTCGCCTGATAAGGGTCTGCTTTCCTTCTTTCTAAAGGGAAGAATCTGCGGACATGAACCCCATTGTAGTGCATACGGCTTGAAATGCTTGCAAAGATACGGATTTTGGCACGGTTTACCAAAAATCTCAGATAGAATTTTACATTGCACACCAACCATGCAAAAGATATATGATCCTGAATATTACCGCGTAGTTTTTTTCTCCAGTGCTCCAATCGGCACTCCATTTTTAACTGCTTTGCACCAGGACCCGAGATTCGAAGTCGTAGGCGTTGTAACCCAAGAAGATAAGCCTGTCGGCAGAGGACTAAAACTCACCCCGAATGTTATCAAACAGACCGCGCTTGAGCTGGGCTTCCAAGAGCAGCAAATCCAAACTCCTCGCAAGATCAACCTTGAAACCTCTATTGAAGGCAAAAACTTCTACGATCGATTGCAAGCAAAATCACCTGATTTCTTGGTAGTTATTGCTTATGGGAAACTCATGCCTGTGAGTCTTCTTGAGCTCCCAACTTTTGCTCCTATCAACGTGCATGGTTCGCTCCTTCCTCAATATCGTGGAGCAAGCCCGCTCCAGTCCGTTTTCCTTGATCAGCAAACGCAGACAGGAATCACCATTATGCATATGGACGCAGGAATGGATACCGGAGCGATCGTCGACCGCCTTGCATTCAAGCTCCCTTTTGACCGAACCGTCAAAACCCTTATCGAAAAGATCCAGCAAACCTGA", "aa": "MLSHLLISTLKKLDRFLARITIGLIRCYQATLSPDKGLLSFFLKGRICGHEPHCSAYGLKCLQRYGFWHGLPKISDRILHCTPTMQKIYDPEYYRVVFFSSAPIGTPFLTALHQDPRFEVVGVVTQEDKPVGRGLKLTPNVIKQTALELGFQEQQIQTPRKINLETSIEGKNFYDRLQAKSPDFLVVIAYGKLMPVSLLELPTFAPINVHGSLLPQYRGASPLQSVFLDQQTQTGITIMHMDAGMDTGAIVDRLAFKLPFDRTVKTLIEKIQQT", "aa_hexdigest": "b200646a4bb730ba0f1b983305123ef4", "start_type": "ATG", "rbs_motif": null, "genes": [ "yidD" ], "truncated": "5-prime", "pseudogene": { "start": 697124, "stop": 698251, "observations": { "mutation": [ 697429 ], "stop": [ 697429 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A660MXR5", "query_cov": 1.0, "subject_cov": 0.6748768472906403, "identity": 0.982, "score": 548.0, "evalue": 5.27e-195, "gene_start": 1, "gene_end": 274, "reference_start": 1, "reference_end": 274, "reference_sequence": "MLSHLLISTLKKLDRFLARITIGLIRCYQATLSPDKGLLSFFLKGRICGHEPHCSAYGLKCLQRYGFWHGLPKISDRILHCTPTMQKIYDPEYYRVVFFSSAPIGTPFLTALHQDPRFEVVGVVTQQDKPVGRGLKLTPNVIKQTALELGFEEQQIQTPRKINLETSIEGKNFYDRLQAKSPDFLVVIAYGKLMPVSLLELPTFAPINVHGSLLPQYRGASPLQSVFLDQQTHTGITVMHMDAGMDTGAIVDRLAFKLPFDRTVKTLIEKIQETGPQFLNDTLRSYAKGSLHATPQDESKSTTCQKITKHDGEIAPARDSLASIYAKYRAYALRPKIRFTHQEKTVVIESLILDADLYAAGKDQPLWDSSYRLHPAIKTLSLKPEGKKSMDRTSFKNGYLKEKKSD" }, "paralog": false, "identity": 0.9733333333333334, "score": 739.0, "evalue": 7.27e-273, "description": "Internal stop codon at 697429. Nonsense mutation around 697429.", "psc": { "uniref90_id": "UniRef90_A0A660MXR5", "gene": "yidD", "product": "membrane protein insertion efficiency factor YidD", "uniref50_id": "UniRef50_A0A7W4HL53", "kegg_orthology_id": "K08998", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A660MXR5", "UniRef:UniRef50_A0A7W4HL53", "KEGG:K08998" ] }, "pscc": { "uniref50_id": "UniRef50_A0A7W4HL53", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7W4HL53" ], "product": "Methionyl-tRNA formyltransferase" } }, "id": "NKOCPLAKPK_1317", "locus": "GCA003260355_01282" }, { "type": "cds", "sequence": "contig_1", "start": 698462, "stop": 698818, "strand": "-", "frame": 3, "gene": null, "product": "CYTH domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAJ0TDD8", "UniRef:UniRef90_A0A328EHG7" ], "nt": "ATGCTTGAACTAGAACTAACCTTCCTGCTTCAGTCATTCCCTCCTGCCTTTGTCGAGGAAGTTCAGACCGGAAACTATCAAAACATTGAATCCAAAAAAGAAATACTTGATTACTATTTTCCCCCAAGCCTGGATCATCCAAGGATCAGACTCAGAAAAGACGGCGAAACTTTTGAGCTCACTAAAAAGCTGGCACTGTCCACAACGGACGCTTCAATTCAAGAGGAACAAACCCTAGTATTAACTGCAGAGGAATATCAATTCTTTGCACAGCTCGAGGGAAAGAAAGTTCATAAAACCAGATACCGCTATGAATATCTCCCAGGTGAGTTTGCTGAAATTGATGTTTTTCACTGA", "aa": "MLELELTFLLQSFPPAFVEEVQTGNYQNIESKKEILDYYFPPSLDHPRIRLRKDGETFELTKKLALSTTDASIQEEQTLVLTAEEYQFFAQLEGKKVHKTRYRYEYLPGEFAEIDVFH", "aa_hexdigest": "2c2cb404ecfbf4ac02c244e20e113bf4", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 698264, "stop": 698818, "observations": { "mutation": [ 698347, 698377, 698380, 698452, 698464 ], "stop": [ 698347, 698377, 698380, 698452, 698464 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EHG7", "query_cov": 1.0, "subject_cov": 0.6413043478260869, "identity": 0.941, "score": 224.0, "evalue": 4.53e-73, "gene_start": 1, "gene_end": 118, "reference_start": 1, "reference_end": 118, "reference_sequence": "MLELEVTFLLQSFPPAFVEEVQTGNYQNIKSKKEILDCYFPRSLDHPRIRLRKNGETFELTKKVALSTTDASIQEEQTLVLTAEEYQFFAQLEGKKIHKTRYRYEYLPGEFAEIDVFHGALSGLVLVDFEFQDPAKKDNFSKPERCGGDITQDATIAGGILAGKSYQDILPLIQRYDYTPLFLT" }, "paralog": false, "identity": 0.907608695652174, "score": 328.0, "evalue": 1.37e-116, "description": "Internal stop codon at 698347, 698377, 698380, 698452, 698464. Nonsense mutation around 698347, 698377, 698380, 698452, 698464.", "psc": { "uniref90_id": "UniRef90_A0A328EHG7", "product": "CYTH domain-containing protein", "uniref50_id": "UniRef50_A0AAJ0TDD8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EHG7", "UniRef:UniRef50_A0AAJ0TDD8" ] } }, "id": "NKOCPLAKPK_1318", "locus": "GCA003260355_01283" }, { "type": "cds", "sequence": "contig_1", "start": 698873, "stop": 699148, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTGATCTCGGGACGGTTGGTTTTCTGCTCCGATGTGGAGTGATGCTCTTGCTCGGACGCCAGCAAAAACAGCTCCATCACTCCTTTAGAGGAGACCACACTTCCGAGGAATGGCAATGTTTTCGTGCTTTGCAAAGGGGAATGCTCGCCTTTCTTGTAATGGGGCTTTTCCTTCACGTCTTTGAAGATAGTATGGTAAATTATTTGTTCTTTATCCCTTATGGTTGCATACTAGGGTATCTTTCAGCTCAGCTCCGTTCGCAAAGGGGATAA", "aa": "MLDLGTVGFLLRCGVMLLLGRQQKQLHHSFRGDHTSEEWQCFRALQRGMLAFLVMGLFLHVFEDSMVNYLFFIPYGCILGYLSAQLRSQRG", "aa_hexdigest": "cbaf9c54090cd775f80dacebf51ccfca", "start_type": "ATG", "rbs_motif": "TTATT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10514.290099999993, "isoelectric_point": 8.61312122344971 }, "id": "NKOCPLAKPK_1319", "locus": "GCA003260355_01284" }, { "type": "cds", "sequence": "contig_1", "start": 699203, "stop": 700165, "strand": "-", "frame": 3, "gene": null, "product": "O-antigen polymerase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MWQ0", "UniRef:UniRef90_A0A328EKM3" ], "nt": "ATGCTCCGAGCAGTGGGAGTTTTCCTTATTTTTCAGCTGGGATATCATATCTTAGTTACTATTTTCGACTATGGAATGGGACGGATTGACCAATCAGCCTTGGCAATGCTTAGAGATGGAGCATGGATTTTGCTAACGCTCATTATTTTCTTGCTCAATACCAGACATCGGAAAGCATACTTCCAGCAATGGCGAAAAGTTTTCTGTGCTTTTGGGGCAGTGCTGATATTTGGTATTCTGACTTCCTTCTTTCTTTCCCACAAAGGTATTAGCGACATTCTGATCGGCATCAAATACGGCCTTTGGCGAATGCTAATCTTGCTTACAGCGAGCGGGATCGGCTTCTTCTCAGCACAGCAAAAAACCAACCTCACTCCTGCTACCAAACGAATCAAACGATCGCTCGTAATCATAGTCCTACTAGGGCGAATCCGACAGCTTGCCAAATTACTCTTCCCTGACTTCTTTTTCTCCCTAGGCTACGGTAAACTTGACAATTTCCATTTCGGCTCCAATCCTCCCATCTATTATCTCACAGGAGAAAACGGCATTCTCAGGCGACAGGGCCTCTTCGCTGGTCCAAATAATTACGGTTATTTCCTTGTGCTCTTTCTCCCTTTGATCCTGCACTTTTTCCGCCTCAGGAATCCAAAGCGAAGTTTCAATCGACTCAAAACCCACCGACAGCAATCCCTTGTCCTCCTGCTCTGGTTTATTACCTTGGGCGCAACATTATCTAGAGCTGCACTCGTTGGAGCAGTGATTGTATTGATGTTGACTCAGTTCTCTTGCCTCAAAAAAAATAAAAAGCTGATGCGAGGACTAGGGATCGTGTGTTGCCTCGGATTGCTTGGCTTCTCAGTTCTCAAACGAGACTCGACAATCGCACACTTAGCCGCCAAATTCAATGGTCTGCTTGAGGTTGCCAACCATCCTCTTGGCTTCGGACTTGGAAGCTCTTGA", "aa": "MLRAVGVFLIFQLGYHILVTIFDYGMGRIDQSALAMLRDGAWILLTLIIFLLNTRHRKAYFQQWRKVFCAFGAVLIFGILTSFFLSHKGISDILIGIKYGLWRMLILLTASGIGFFSAQQKTNLTPATKRIKRSLVIIVLLGRIRQLAKLLFPDFFFSLGYGKLDNFHFGSNPPIYYLTGENGILRRQGLFAGPNNYGYFLVLFLPLILHFFRLRNPKRSFNRLKTHRQQSLVLLLWFITLGATLSRAALVGAVIVLMLTQFSCLKKNKKLMRGLGIVCCLGLLGFSVLKRDSTIAHLAAKFNGLLEVANHPLGFGLGSS", "aa_hexdigest": "31b5f395e1db610115e8020d09d2db16", "start_type": "ATG", "rbs_motif": "TTATT", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 698900, "stop": 700165, "observations": { "mutation": [ 699205 ], "stop": [ 699205 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EKM3", "query_cov": 1.0, "subject_cov": 0.7441860465116279, "identity": 1.0, "score": 626.0, "evalue": 9.94e-225, "gene_start": 1, "gene_end": 320, "reference_start": 1, "reference_end": 320, "reference_sequence": "MLRAVGVFLIFQLGYHILVTIFDYGMGRIDQSALAMLRDGAWILLTLIIFLLNTRHRKAYFQQWRKVFCAFGAVLIFGILTSFFLSHKGISDILIGIKYGLWRMLILLTASGIGFFSAQQKTNLTPATKRIKRSLVIIVLLGRIRQLAKLLFPDFFFSLGYGKLDNFHFGSNPPIYYLTGENGILRRQGLFAGPNNYGYFLVLFLPLILHFFRLRNPKRSFNRLKTHRQQSLVLLLWFITLGATLSRAALVGAVIVLMLTQFSCLKKNKKLMRGLGIVCCLGLLGFSVLKRDSTIAHLAAKFNGLLEVANHPLGFGLGSSGPAVHHSGNLLPENYYLQLMLDLGTVGFLLRCGVMLLLGRQQKQLHHSFRGDHTSEEWQCFRALQRGMLAFLVMGLFLHVFEDSMVNYLFFIPYGCILGYLSAQLRSQRG" }, "paralog": false, "identity": 0.997624703087886, "score": 843.0, "evalue": 1.17e-312, "description": "Internal stop codon at 699205. Nonsense mutation around 699205.", "psc": { "uniref90_id": "UniRef90_A0A328EKM3", "product": "O-antigen polymerase", "uniref50_id": "UniRef50_A0A660MWQ0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EKM3", "UniRef:UniRef50_A0A660MWQ0" ] }, "pscc": { "uniref50_id": "UniRef50_A0A660MWQ0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MWQ0" ], "product": "O-antigen ligase domain-containing protein" } }, "id": "NKOCPLAKPK_1320", "locus": "GCA003260355_01285" }, { "type": "cds", "sequence": "contig_1", "start": 700560, "stop": 701054, "strand": "+", "frame": 3, "gene": null, "product": "N(6)-L-threonylcarbamoyladenine synthase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2G6CC49", "UniRef:UniRef90_A0A660MXI9" ], "nt": "TTGCCCGGATCACTCGTAGTAGGGAAGACCGTTGCCTATGAATTGGGAGCGTTTTTGGGGAAAGAAGTAGTGCCGGTTCATCATATTGTGGGGCATATTTTTTCTATCTTACTTGAGAGGAAGCCCGAAGAGCTATCATTTCCTTTGGTTATCTTGACGGCATCAGGAGGGCACAATGATATCTATCTGATAGAAAAAAGAGAAGATGCTGAATCTAGACAGAATTTTGGCTCTTTTGAATTTTCAGGCTTCCGCATTACGAAAGTTGGTTGGACACTTGATGATGCTGCTGGAGAGTGTTTTGATAAGGTTTCAAGGATGCTCGGTGGACCTTATCCTGGTGGACTCCGAATCTCTCAGAAAGCTTCTCTCGGGAAACCTAATCCTCTCGTTCAATTTAAGAGAATCTTCCTCGCTCATGAGAAATTTGAATTTAGTTTTTCTGGGATGAAGAGTCAGGTTTCTTTTCTTTTGGAACATTTAGAGAGGGACTGA", "aa": "MPGSLVVGKTVAYELGAFLGKEVVPVHHIVGHIFSILLERKPEELSFPLVILTASGGHNDIYLIEKREDAESRQNFGSFEFSGFRITKVGWTLDDAAGECFDKVSRMLGGPYPGGLRISQKASLGKPNPLVQFKRIFLAHEKFEFSFSGMKSQVSFLLEHLERD", "aa_hexdigest": "26944358afa6dba586b2990d9fa3c2a9", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 700311, "stop": 701342, "observations": { "mutation": [ 700377, 700437, 700506, 701052 ], "stop": [ 700377, 700437, 700506, 701052 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A660MXI9", "query_cov": 1.0, "subject_cov": 0.478134110787172, "identity": 0.982, "score": 325.0, "evalue": 8.12e-110, "gene_start": 1, "gene_end": 164, "reference_start": 84, "reference_end": 247, "reference_sequence": "MKTLAIETSCDDTSLALVNYEDGSFKVEQLLAYSQIQEHQKFGGVVPELASRLHSEKIIALLQTIGFDEIQKVDFISVTTQPGLPGSLVVGKTVAYELGAFLGKEVVPVHHIVGHIFSILLERKPEELSFPLVILTASGGHNDIYLIEKREDAESRQDFGSFEFSGFRITKVGWTLDDAAGECFDKVSRMLGGPYPGGLWISQKASLGKPNPLVQFKRIFLAHEKFEFSFSGMKSQVSFLLEHLERDGITLTEELVCDIAYEFQEAVVEVLSKKLIQAGEHFGAKALALAGGVSANTRLCNAILELLTKKQLACPFFIPVKKVYSTDNGAMIGLAGLLTKMEV" }, "paralog": false, "identity": 0.9650145772594753, "score": 643.0, "evalue": 7.58e-238, "description": "Internal stop codon at 700377, 700437, 700506, 701052. Nonsense mutation around 700377, 700437, 700506, 701052.", "psc": { "uniref90_id": "UniRef90_A0A660MXI9", "product": "N(6)-L-threonylcarbamoyladenine synthase", "uniref50_id": "UniRef50_A0A2G6CC49", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A660MXI9", "UniRef:UniRef50_A0A2G6CC49" ] }, "pscc": { "uniref50_id": "UniRef50_A0A2G6CC49", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2G6CC49" ], "product": "N(6)-L-threonylcarbamoyladenine synthase" } }, "id": "NKOCPLAKPK_1321", "locus": "GCA003260355_01286" }, { "type": "cds", "sequence": "contig_1", "start": 701061, "stop": 701342, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF22521.2" ], "nt": "TTGACGGACGAACTTGTCTGTGATATTGCTTATGAGTTTCAGGAAGCTGTCGTGGAAGTGCTTTCTAAGAAGCTTATTCAGGCAGGAGAACATTTTGGAGCGAAAGCATTGGCTTTGGCGGGAGGAGTATCGGCAAATACTAGGCTTTGCAACGCAATCCCAGAGCTTTTAAGTAAAAAACAGCTTACTTGTCCGTTTTTTATCCCTGTGAAAAAGGTTTATTCAACCGATAATGGAGCGATGATTGGACTTGCAGGACTGTTAACTAAGATCGAAGTGTAA", "aa": "MTDELVCDIAYEFQEAVVEVLSKKLIQAGEHFGAKALALAGGVSANTRLCNAIPELLSKKQLTCPFFIPVKKVYSTDNGAMIGLAGLLTKIEV", "aa_hexdigest": "f81a3ebd5f8ac657b6f1375f1fd22848", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF22521.2", "name": "HypF_C_2", "length": 57, "aa_cov": 0.6129032258064516, "hmm_cov": 1.0, "evalue": 8.856744207681178e-08, "score": 28.622596740722656, "start": 8, "stop": 64 } ], "seq_stats": { "molecular_weight": 9955.611699999992, "isoelectric_point": 5.732519340515138 }, "id": "NKOCPLAKPK_1322", "locus": "GCA003260355_01287" }, { "type": "cds", "sequence": "contig_1", "start": 701463, "stop": 701819, "strand": "+", "frame": 3, "gene": null, "product": "Ribonuclease", "db_xrefs": [ "EC:3.1.26.4", "KEGG:K03470", "SO:0001217", "UniRef:UniRef50_A0A328EGV2", "UniRef:UniRef90_A0A328EGV2" ], "nt": "GTGATTAGCAAGATTTCTCCTCAAGCTCTCGCCAAGCATCCCCTCTTCCAAGACAGCAAAAAATTGACTGCGAAAAAAAGGCAACTTGCGTTTTCTGAAATTCAAAGTTTAGCGGAGAGAAAAAAGATTTCCATCGCTATTGCGAGCGTTGAAGCGGGGATAATAGATTGCTATGGCGTTACTCAAGCGATTGGCATTGCGATCTGCAAGGGGCTTTATCAGCTGATTTGTCCTCTGATGAAAAGGACTTTGAAGCAAGAGTTTTTTCTTTCTGAACTGAAGGATTTGATACGTGAGTATGAGGATAGGCATCAAGAGAAAATAAGGCTAATTCTGGATGGGAAATCAGATTTTTGA", "aa": "MISKISPQALAKHPLFQDSKKLTAKKRQLAFSEIQSLAERKKISIAIASVEAGIIDCYGVTQAIGIAICKGLYQLICPLMKRTLKQEFFLSELKDLIREYEDRHQEKIRLILDGKSDF", "aa_hexdigest": "4369605e26351b3c9625b3579432c29c", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 701400, "stop": 702119, "observations": { "mutation": [ 701424, 701442, 701817, 701823, 701961, 701985 ], "stop": [ 701424, 701442, 701817, 701823, 701961, 701985 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EGV2", "query_cov": 1.0, "subject_cov": 0.49372384937238495, "identity": 0.992, "score": 223.0, "evalue": 3.84e-72, "gene_start": 1, "gene_end": 118, "reference_start": 22, "reference_end": 139, "reference_sequence": "MDIFFDEAGRGPLFGPLYIGLVISKISPQALAKHPLFQDSKKLTAKKRQLAFSEIQSLAERKKISIAIASVEAGIIDCYGVTQAIGIAICKGLYQLICPLMKRTLKQEFFLSELKDLIREYEDRHQEKIRLILDGKSDFGLGKCLEIETKTIVHGDAQVKEIAIASILAKVSRDQYLEELSHRYPAYGLEKHKGYGTKGHYSKIQTFGTTEEHRKLFLKKLFPKWTIQALDFSTYSFKI" }, "paralog": false, "identity": 0.9748953974895398, "score": 452.0, "evalue": 9.74e-165, "description": "Internal stop codon at 701424, 701442, 701817, 701823, 701961, 701985. Nonsense mutation around 701424, 701442, 701817, 701823, 701961, 701985.", "psc": { "uniref90_id": "UniRef90_A0A328EGV2", "product": "Ribonuclease", "ec_ids": [ "3.1.26.4" ], "uniref50_id": "UniRef50_A0A328EGV2", "kegg_orthology_id": "K03470", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EGV2", "EC:3.1.26.4", "UniRef:UniRef50_A0A328EGV2", "KEGG:K03470" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EGV2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EGV2" ], "product": "Ribonuclease" } }, "id": "NKOCPLAKPK_1323", "locus": "GCA003260355_01288" }, { "type": "cds", "sequence": "contig_1", "start": 702168, "stop": 702350, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCAATTTTATTTCTCTGTGATAGTGAGAATGTCAACAACGTTCTGGTTTTGGATTATTATTGCTTTAATACTTCTGCTTTTGATCCGTATCTTTAGGAGATATATTGGAAGAATCATCCTGCTTCTTCTTTTTCTTTTGGTAGTTTTCTTTGTTTATAAGCGGTTAAATCCTCTCCAATGA", "aa": "MQFYFSVIVRMSTTFWFWIIIALILLLLIRIFRRYIGRIILLLLFLLVVFFVYKRLNPLQ", "aa_hexdigest": "15e7e46ccdb9eef45a2c2dc97c24eab1", "start_type": "GTG", "rbs_motif": "TTATT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7455.271199999994, "isoelectric_point": 11.722947120666507 }, "id": "NKOCPLAKPK_1324", "locus": "GCA003260355_01289" }, { "type": "cds", "sequence": "contig_1", "start": 702369, "stop": 702650, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGGAGTTCCTGCACGTTCAGTAAACTTTGTCAATTTTTATCTCCTAAAAAATGATGTTGAGGCTCCTCTTCTTGAGGAAAGAGCGTCTACTAATGAACTGAAAGTATTGTCAGGGAATGTTTCTGATCATCTTAAAAATTCAGATAACTCAGCTAGTCAAGCTCCAATACTTAGGAAAGATAGCGAATCCTCTTCAGAGAGTGGATTATCTTCAGGTTCTAAAGCTGTAGAACTTGTTATTCTCAGTCAAGATACGACTCCTTCTGTGCAATCATGA", "aa": "MKGVPARSVNFVNFYLLKNDVEAPLLEERASTNELKVLSGNVSDHLKNSDNSASQAPILRKDSESSSESGLSSGSKAVELVILSQDTTPSVQS", "aa_hexdigest": "4cfde79e08dc81a2ce574a38ba785490", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9920.867399999997, "isoelectric_point": 4.993441963195799 }, "id": "NKOCPLAKPK_1325", "locus": "GCA003260355_01290" }, { "type": "cds", "sequence": "contig_1", "start": 702687, "stop": 702974, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAAGCAAGAGTTCGTCCTTGAAGCTCAAGAAACTTCTTATGAAGCGAAAGAATCCTCTCTCAATACTAAAGAGACTTTAGATGTAACTAAACAAGCTTCTCAAGGAGCTCAAGCACCAACTGTGAAAATCCCAGTCGAAACTCACGAAATTAAAGAACCTTCTCACAAGCAGAACACAGCTTCTGCTCCAATAAGCAGAACAACCAATAAAGTAGCATCCACCTCTACTTATCATTCAAAAGGGCTTACTGCTGCTGACTATGAGGAGGCTTGGGCAATTTTTTGA", "aa": "MKQEFVLEAQETSYEAKESSLNTKETLDVTKQASQGAQAPTVKIPVETHEIKEPSHKQNTASAPISRTTNKVASTSTYHSKGLTAADYEEAWAIF", "aa_hexdigest": "69bbfaf585f5de3c33d785153087ecb4", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10442.388299999993, "isoelectric_point": 5.5600698471069325 }, "id": "NKOCPLAKPK_1326", "locus": "GCA003260355_01291" }, { "type": "cds", "sequence": "contig_1", "start": 703147, "stop": 703608, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBFE28D", "UniRef:UniRef100_A0A328EHP1", "UniRef:UniRef50_A0A328EHP1", "UniRef:UniRef90_A0A328EHP1" ], "nt": "ATGGCAACTAAAGAATGTTCTTGCTATCATTCGTATCCCGCTATTGAAGCTGTACTTCAAGCTGGAGCTGGGATGACCATCTCATTTCGGAATGGTAGAGGTTTATGCGAGGTCAGAATTGAGAAAGGAGAGAAAGTTTTGGTCTGTGTAGAGGGCTTTCGTGTTCAGGTTGCACTTCAACGGGCAAATCAAGAGCTTCTTGGTGGACCTTGGAATACTGGAGTCGCACCTAACCAAACGGTGGAAAGCTTCGTTGGTTTGGTAGACCTGAACGAAATCTCTAAGGATAGATTTGATTCTTATATCTTCCTTTGGAAGGTTACTCGGATTTTTTTTCATGAGGAAGTTGGATTCCTGTGTAGGGCAGGTGCAACCGAATCTTGTATATGGGGAAGTGGGAAAACCATGGCAGATGCTGTCATTGATTGCTGCCGAGCTACGAAGAATCCGACGAATAACTAG", "aa": "MATKECSCYHSYPAIEAVLQAGAGMTISFRNGRGLCEVRIEKGEKVLVCVEGFRVQVALQRANQELLGGPWNTGVAPNQTVESFVGLVDLNEISKDRFDSYIFLWKVTRIFFHEEVGFLCRAGATESCIWGSGKTMADAVIDCCRATKNPTNN", "aa_hexdigest": "a1c553795378bbe14bb8952915f8455a", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000DBFE28D", "uniref100_id": "UniRef100_A0A328EHP1", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBFE28D", "UniRef:UniRef100_A0A328EHP1" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EHP1", "uniref90_id": "UniRef90_A0A328EHP1" }, "psc": { "uniref90_id": "UniRef90_A0A328EHP1", "uniref50_id": "UniRef50_A0A328EHP1" }, "pscc": { "uniref50_id": "UniRef50_A0A328EHP1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EHP1" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16860.129999999994, "isoelectric_point": 6.123855781555174 }, "id": "NKOCPLAKPK_1327", "locus": "GCA003260355_01292" }, { "type": "cds", "sequence": "contig_1", "start": 703738, "stop": 705105, "strand": "-", "frame": 1, "gene": "ftsK", "product": "FtsK domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EKC5", "UniRef:UniRef90_A0A328EKC5" ], "nt": "GTGGAAAAGGCACAAGCTTTGCAAACCAAGCTCGGGGAATTTGGGGTTCCAATTGCGATTGAGGGATTTGATATTGGACCGTCTATCGTGCAGATCAAGATTAAACCTGATGAGGGGATCAGAATTTCTGCTATTGAAGGGCTGGCAAATGATATCAAGCTTTCCCTCAAATCTAAATCGCTCAGAATCATCGCTCCGATCCCAGGAACCGATCTCGTGGGAATCCAAATCCCAAATCCAAAACCAAATATGGTGAGGCTCGGCGATGTGATCAATACCAGCGAATTTTCTACGGAGATGAAAAAGAATGCAACCAATCTTTCTCTTGGGAAAGGGATTGATGGAAGTGTGCAGGTGAGACCGCTTGAAAAGATGCCACATTTGCTTGTTGCCGGTGCGACAGGAAGCGGAAAGTCTGTAGGAATCAACGATTTTATCGTTTCGCTGATGTTTCAGAATACTCCTGCTGAACTCAAGTTTCTTATGGTCGATCCGAAGCAAGTTGAACTAGAAATGTATTCAGGTTTGCCGTATTTGCTCGCTCCGATCGTGTATGATAGTGGGAAAGCGATCAAACTCCTTCAGCGAACGGTTGAGGAGATGGAAAAAAGATATACGACGCTCAAAGACCAAAGAGTAAGAAATCTGGATGAATACAACGAAAAGATGAGTGCCGAAGGACAAGAGAAAATGTATCGTATTGTCTTTATCATTGATGAAATGGCGGATATGATGCTCTCTAGCTCTGCCAATAGAAAAGAAGTTGAAAACTGCATTAACCGTCTTGCTGCAAAAGCCAGAGCCGTGGGAATCCATCTTATCCTCGCAACACAAAGACCATCTGTGAATGTGATTACCGGTCTGATCAAAGCAAATATACCGACGAGAATTGCCTTTGGAGTGGTTTCTGAGGTGGATAGTAGGACGATTCTTGGTCGTAAAGGAGCAGAAGACCTCGTTGGAAAGGGAGATATGCTCTATATGGATCCAAGCACGAAATTCCCAATCAGAATCCAGGCTCCGTTTATTGCAACAGAAGAGATTGAAAAGGTCGTTGCTCAGCTGAGAAATAAATATATGCAAGGACTAAGTGAAGACGATATTTATAATCAGGAAATTATTACAGCATTAGAAAGCAGACTGGAAACTGCGAAAGGTTCCTTTGCTGGAGGGGATTCTGGAGATGATGAAGACCTCGTCAATCAAGCCATCCAAGTCATCGCGGAAACCAGAAAAGCTTCTGCAACCTTGCTCCAGAGAAAGCTCAATGTTGGATTTGCAAGGGCTGCAAGAATTATGGATATTTTGGAGGAAAGAGGAGTGATAGGTCCTCAAGAGGGGGCAAAACCGAGAGATATTTTCATCTAA", "aa": "MEKAQALQTKLGEFGVPIAIEGFDIGPSIVQIKIKPDEGIRISAIEGLANDIKLSLKSKSLRIIAPIPGTDLVGIQIPNPKPNMVRLGDVINTSEFSTEMKKNATNLSLGKGIDGSVQVRPLEKMPHLLVAGATGSGKSVGINDFIVSLMFQNTPAELKFLMVDPKQVELEMYSGLPYLLAPIVYDSGKAIKLLQRTVEEMEKRYTTLKDQRVRNLDEYNEKMSAEGQEKMYRIVFIIDEMADMMLSSSANRKEVENCINRLAAKARAVGIHLILATQRPSVNVITGLIKANIPTRIAFGVVSEVDSRTILGRKGAEDLVGKGDMLYMDPSTKFPIRIQAPFIATEEIEKVVAQLRNKYMQGLSEDDIYNQEIITALESRLETAKGSFAGGDSGDDEDLVNQAIQVIAETRKASATLLQRKLNVGFARAARIMDILEERGVIGPQEGAKPRDIFI", "aa_hexdigest": "9203a1c93091ff0321e5286ae05e292f", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A554LLW0", "query_cov": 0.810989010989011, "subject_cov": 0.9866310160427807, "identity": 0.509, "score": 320.0, "evalue": 4.56e-103, "valid": false, "product": "DNA segregation ATPase FtsK/SpoIIIE, S-DNA-T family", "uniref50_id": "UniRef50_A0A7Y5SG32" }, "genes": [ "ftsK" ], "truncated": "3-prime", "pseudogene": { "start": 703738, "stop": 705405, "observations": { "mutation": [ 705183, 705270 ], "stop": [ 705183, 705270 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EKC5", "query_cov": 1.0, "subject_cov": 0.6182065217391305, "identity": 0.971, "score": 846.0, "evalue": 1.32e-304, "gene_start": 1, "gene_end": 455, "reference_start": 282, "reference_end": 736, "reference_sequence": "MVLARRRKTSRARTFTLNKTHIGIGMLGLGILYFFSRAMAQGSPVFEFFTKYSKIAFGEIGLLFFFGLCIVIGTMILLKGHLAKFLVKYFFSLLMLVSAVLNVRLRDATMPIKWLENGGYFSWPLIWLLEKGLKNGDGSYITAIKAIVIVFLIVLIGYLLYVFNVRLPKLPKISIEESPKPRENKKADSKEQERPTITISRPEISTADIMEKVSQATGKISKATSGGGSLLKDMLKSKVEQKIEEKKPKPVIHYSGDKPTFGTSLLESGLGQEQTIDENFLVEKAKALQTKLGEFGVPIAIEGFDIGPSIVQIKIKPDEGIRISAIEGLANDIKLSLKSKSLRIIAPIPGTDLVGIQIPNPKPNMVRLGDVINTSEFSEGMKKNLTNLSLGKGIDGSVQIRPLEKMPHLLVAGATGSGKSVGINDFIVSLMFQNTPAELKFLMVDPKQVELEMYSGLPYLLAPIVYDSGKAIKLLQRTVEEMEKRYTTLKDQRVRNLDEYNEKMQKEGQEKMYRIVFIIDEMADMMLSSSANRKEVENCINRLAAKARAVGIHLILATQRPSVNVITGLIKANIPTRIAFGVVSEVDSRTILGRKGAEDLVGKGDMLYMDPSTKFPIRIQAPFIATEEIEKVVAQLRNKYMQGLSEEDIYNQEIIAALESRLETAKGSFAGGESGDDEDLVNQAIQVIAETRKASATLLQRKLNVGFARAARIMDILEERGIIGPQEGAKPRDIFL" }, "paralog": false, "identity": 0.9513513513513514, "score": 1016.0, "evalue": 0.0, "description": "Internal stop codon at 705183, 705270. Nonsense mutation around 705183, 705270.", "psc": { "uniref90_id": "UniRef90_A0A328EKC5", "product": "FtsK domain-containing protein", "uniref50_id": "UniRef50_A0A328EKC5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EKC5", "UniRef:UniRef50_A0A328EKC5" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EKC5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EKC5" ], "product": "FtsK domain-containing protein" } }, "id": "NKOCPLAKPK_1328", "locus": "GCA003260355_01293" }, { "type": "cds", "sequence": "contig_1", "start": 705268, "stop": 705495, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCTGTTGGTTGGGTATTTGCTTTATATTTTCAATGTCCGCCTGCCAAAACTTCCCAAAATCAATATCGAGACTCCTGATACTCCTATCAAAAAAGAAAAAAAAGCTGACTCCAAAGAACAGGAAAGACCTACGATTACGATCACGAGACCCGAAATCTCTACCGAAGCGATTATGGAAAAAGTTTCTCAAGCCACCGGAAAAATCGCCAAGTCTAGTTCTGGTTGA", "aa": "MLLVGYLLYIFNVRLPKLPKINIETPDTPIKKEKKADSKEQERPTITITRPEISTEAIMEKVSQATGKIAKSSSG", "aa_hexdigest": "8a715f6fd6f93423bbe49d3cc89a4026", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8385.748799999998, "isoelectric_point": 9.521545219421387 }, "id": "NKOCPLAKPK_1329", "locus": "GCA003260355_01294" }, { "type": "cds", "sequence": "contig_1", "start": 705598, "stop": 705741, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATTGGGCTCCTCATTTTGGCTAAAGGACATCTGACCAAATTCCTAGTAAAATACTTTTTCTCAGTCTTAATGCTGACTTCTGCCTGGCTAAATATCCGCTTACGAGATGTTACAACCCCTATTAAGTGGCTTGAAAACTGA", "aa": "MIGLLILAKGHLTKFLVKYFFSVLMLTSAWLNIRLRDVTTPIKWLEN", "aa_hexdigest": "19d1b8b1eb3f7dc4b8ebb5fb2a841306", "start_type": "GTG", "rbs_motif": "TTTTT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5505.671399999999, "isoelectric_point": 10.165906715393067 }, "id": "NKOCPLAKPK_1330", "locus": "GCA003260355_01295" }, { "type": "cds", "sequence": "contig_1", "start": 706280, "stop": 706618, "strand": "-", "frame": 3, "gene": null, "product": "Superoxide dismutase", "db_xrefs": [ "EC:1.15.1.1", "KEGG:K04564", "SO:0001217", "UniRef:UniRef50_A0A376F9B2", "UniRef:UniRef90_A0A328EKP8" ], "nt": "TTGAGTCCAAAACATGATCAAGTTCCTAGTGGGAAATTGGCAGAACTCATTGATCAGGATTTTGGATCTTTTGCTAGTTTCAAAGAGCAGTTTATTTCTTCAGCAGTTGGAAATTTTGGTTCTGGTTGGACTTGGCTAGTTTTGAATCAAGAAGGAAAATTAGAAATTCTGAATATGTCGAACGCTCAAAATCCGCTTCACTTGCCAGTAAAAATCCTTCTTGGAGTAGATGTGCGAGAGCATGCCTATTATATTGATACGAGAAATAATAGAGGAGCATATTTGGAGAATTTTTTCCATGTGATCAATTGGGGCTTCGTTGCTGAAAATTTGGGGTAA", "aa": "MSPKHDQVPSGKLAELIDQDFGSFASFKEQFISSAVGNFGSGWTWLVLNQEGKLEILNMSNAQNPLHLPVKILLGVDVREHAYYIDTRNNRGAYLENFFHVINWGFVAENLG", "aa_hexdigest": "2d30c5d24b7205c6fe3a2b73f815b6dd", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI000556B0FF", "query_cov": 0.9910714285714286, "subject_cov": 0.9652173913043478, "identity": 0.5770000000000001, "score": 126.0, "evalue": 1.31e-35, "valid": false, "product": "Fe-Mn family superoxide dismutase", "ec_ids": [ "1.15.1.1" ], "uniref50_id": "UniRef50_F3LG87", "kegg_orthology_id": "K04564" }, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 706280, "stop": 706858, "observations": { "mutation": [ 706675 ], "stop": [ 706675 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EKP8", "query_cov": 1.0, "subject_cov": 0.5833333333333334, "identity": 0.9640000000000001, "score": 226.0, "evalue": 5.61e-74, "gene_start": 1, "gene_end": 112, "reference_start": 81, "reference_end": 192, "reference_sequence": "MTFQKIALPYPKDALAPYISEETMSYHYEKHHQAYADKLNQLIENTDFAGKDIVDIVKNSNGALYNNAAQFWNHNFFWNCLSSKHDQVPGGKLAELIDQDFGSFASFKEQFISSAVGNFGSGWTWLVLNQEGKLEILNMSNAQNPLHLPVKILLGVDVREHAYYIDTRNNRGAYLENFFHVINWDFVAENLG" }, "paralog": false, "identity": 0.9791666666666666, "score": 393.0, "evalue": 4e-142, "description": "Internal stop codon at 706675. Nonsense mutation around 706675.", "psc": { "uniref90_id": "UniRef90_A0A328EKP8", "product": "Superoxide dismutase", "ec_ids": [ "1.15.1.1" ], "uniref50_id": "UniRef50_A0A376F9B2", "kegg_orthology_id": "K04564", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EKP8", "EC:1.15.1.1", "UniRef:UniRef50_A0A376F9B2", "KEGG:K04564" ] }, "pscc": { "uniref50_id": "UniRef50_A0A376F9B2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A376F9B2" ], "product": "Superoxide dismutase" } }, "id": "NKOCPLAKPK_1331", "locus": "GCA003260355_01296" }, { "type": "cds", "sequence": "contig_1", "start": 706673, "stop": 706858, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00081.27" ], "nt": "ATGACCTTTCAGAAAATAGCACTTCCTTATCCAAAAGATGCACTCGCTCCTTACATCTCTGAAGAGACGATGAGCTATCATTATGAAAAACATCATCAGGCGTATGCTGATAAACTTAATCAATTGATTGAAAATACCGATTTCGCTGGAAAGGATATCGTAGATATTGTAAAAAACTCAAATTGA", "aa": "MTFQKIALPYPKDALAPYISEETMSYHYEKHHQAYADKLNQLIENTDFAGKDIVDIVKNSN", "aa_hexdigest": "2752e0328074f139d74857b79547319a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00081.27", "name": "Sod_Fe_N", "length": 56, "aa_cov": 0.9180327868852459, "hmm_cov": 1.0, "evalue": 1.022417256251363e-19, "score": 67.27167510986328, "start": 3, "stop": 58 } ], "seq_stats": { "molecular_weight": 7059.851900000003, "isoelectric_point": 5.392792701721191 }, "id": "NKOCPLAKPK_1332", "locus": "GCA003260355_01297" }, { "type": "cds", "sequence": "contig_1", "start": 706915, "stop": 707661, "strand": "-", "frame": 1, "gene": "gpmA", "product": "2,3-diphosphoglycerate-dependent phosphoglycerate mutase", "db_xrefs": [ "EC:5.4.2.11", "GO:0004619", "GO:0006096", "KEGG:K01834", "SO:0001217", "UniParc:UPI000DBFDB84", "UniRef:UniRef100_A0A328EH71", "UniRef:UniRef50_Q8P7A1", "UniRef:UniRef90_A0A328EH71" ], "nt": "ATGAAAAAACTAGTTCTTCTCCGTCATGGAGAAAGTACTCGAAACCAAGAGAATAGATTTACTGGACGAACGGATGTTGATCTTACCGAAAAAGGGCTCCAAGAAGCTAAAAAAGCAGGTGCACTTTTGAAGGCTGAAAATCTCCACTTCTCGAGAGCTTTTACCTCTTACCTCAAAAGAGCTATCAAGACCTTGAATACCGTGCTAGATACGATGGATTTAGATTGGATCCCTGTAGAAAAATCTCGGAGACTCAATGAAAAGCATTATGGAATGCTCCAGGGGCTCAATAAAGCCGAAACCGCACAAAAATACGGAGACGAACAGGTACTTCTTTGGAGAAGGAGCTACGATATCCCGCCAACTCCACTTGAAAAAGACGATCCACGTTCTCCTTTTCAGGATCCTCGTTATGCAAGGGTAGCACCTCAAGACTTGCCACTAACAGAATCCCTTAGAGACTGTGTAGGTCGTGCATTGCCTTATTTGCAGGAAACGATATTGCCAGCACTAGAGAGAGAGGATACGATTTTGGTGGCTGCACATGGAAATAGTTTGAGAGGAATTATTAAGGTGCTAAAAGGAATGTCAGACGAAGATATTCTAAAGCTCAACCTCCCAACAGCAGTTCCTTATGTATTTGAATTTGACGATGAAATGAAGCTTACGAAGGATTATTTCCTTGGAGATCCAGAAGAGGTGAAAAAACTGATGGAAGCTGTAGCAAACCAGGGGAAAAAAGCATAA", "aa": "MKKLVLLRHGESTRNQENRFTGRTDVDLTEKGLQEAKKAGALLKAENLHFSRAFTSYLKRAIKTLNTVLDTMDLDWIPVEKSRRLNEKHYGMLQGLNKAETAQKYGDEQVLLWRRSYDIPPTPLEKDDPRSPFQDPRYARVAPQDLPLTESLRDCVGRALPYLQETILPALEREDTILVAAHGNSLRGIIKVLKGMSDEDILKLNLPTAVPYVFEFDDEMKLTKDYFLGDPEEVKKLMEAVANQGKKA", "aa_hexdigest": "b112f45b41d6ba610c8324ae20930ebc", "start_type": "ATG", "rbs_motif": "TTATT", "ups": { "uniparc_id": "UPI000DBFDB84", "uniref100_id": "UniRef100_A0A328EH71", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBFDB84", "UniRef:UniRef100_A0A328EH71" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EH71", "uniref90_id": "UniRef90_A0A328EH71" }, "psc": { "uniref90_id": "UniRef90_A0A328EH71", "gene": "gpmA", "product": "2,3-diphosphoglycerate-dependent phosphoglycerate mutase", "ec_ids": [ "5.4.2.11" ], "uniref50_id": "UniRef50_Q8P7A1", "kegg_orthology_id": "K01834", "go_ids": [ "GO:0004619", "GO:0006096" ] }, "pscc": { "uniref50_id": "UniRef50_Q8P7A1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q8P7A1" ], "product": "2,3-bisphosphoglycerate-dependent phosphoglycerate mutase" }, "genes": [ "gpmA" ], "id": "NKOCPLAKPK_1333", "locus": "GCA003260355_01298" }, { "type": "cds", "sequence": "contig_1", "start": 707802, "stop": 708452, "strand": "+", "frame": 3, "gene": null, "product": "DUF4012 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EL65", "UniRef:UniRef90_A0A328EL65" ], "nt": "ATGAAAAAAATCGGAATTGAACCACTGAAAAAGGAGTCAATTTCTTTTGGAAAGTTTGTTTTGCATACGGTTTTTCTCTCGCTTTTGGTGCTGAGTTTTACTGTTTTTTCTTTGTACTCAAAAATTACTGAACGGTTTTTTTCAAATCATAAGATTGATATCTTCACTGAACTACCTCAGGTTGCTTCTTATTTTTGGGTTGTTGACCCGGAGGTAGCTCGCCAGATTTTGCAACTTGATCTCATCATAAAAGATTACCTGTCTGGTGCTAACGTCCTGAAAACTAGAGAAGAGGAACTTACTGCACTTTGGAGCTATGCGAAAGAGAATAAAGACTACCTCACAAAGTTAGGATTTGGGAACTATAAGAGGATTTTGAAGATGCTTTCAGATGCACGACCACTGAGAGAAGAAATTTTCCAACTCCTCGGGAAAGATCAGCGTTTTAATTACCTTGTTCCTCTCCAAAACAGTAATGAATCTCGTCCTAATGGGGGATTCTTTGGGTCTTTTGCGTTTATTAGCCTTTCTGGAGGGCATATTGTCGATATGCAGATTATTGATTCTTATCTCCCTGATATGCTTGCTCCTAATACCCGTGTTCCTCTGCCTGAACGAACTCAGGGATTTCTGACAGAGAAAGCTGCGTGA", "aa": "MKKIGIEPLKKESISFGKFVLHTVFLSLLVLSFTVFSLYSKITERFFSNHKIDIFTELPQVASYFWVVDPEVARQILQLDLIIKDYLSGANVLKTREEELTALWSYAKENKDYLTKLGFGNYKRILKMLSDARPLREEIFQLLGKDQRFNYLVPLQNSNESRPNGGFFGSFAFISLSGGHIVDMQIIDSYLPDMLAPNTRVPLPERTQGFLTEKAA", "aa_hexdigest": "cf754fb9ae48ef3ff2c11e143c40c3af", "start_type": "ATG", "rbs_motif": "TTATT", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 707802, "stop": 708755, "observations": { "mutation": [ 708450, 708474, 708492 ], "stop": [ 708450, 708474, 708492 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EL65", "query_cov": 1.0, "subject_cov": 0.4044943820224719, "identity": 1.0, "score": 424.0, "evalue": 1.68e-145, "gene_start": 1, "gene_end": 216, "reference_start": 1, "reference_end": 216, "reference_sequence": "MKKIGIEPLKKESISFGKFVLHTVFLSLLVLSFTVFSLYSKITERFFSNHKIDIFTELPQVASYFWVVDPEVARQILQLDLIIKDYLSGANVLKTREEELTALWSYAKENKDYLTKLGFGNYKRILKMLSDARPLREEIFQLLGKDQRFNYLVPLQNSNESRPNGGFFGSFAFISLSGGHIVDMQIIDSYLPDMLAPNTRVPLPERTQGFLTEKAAGFIGGNKFGFTDKDGRNLKQLYEKIFHTAFDPEKREKMFNQEQWNQLFQKNIKGIIFLDSELITYLLPAFRSKTREWQFVNANVDLIRGENRSNKKEFYIKDLQRYLKDNALTLAKSTINNIQELLHKGFINIYLSNVSDEMRTFLKDYDLTNVYNPDFFYFFHINSSFNKSDGFVKKQVEVLGPQGNVLLSTEERKLDIHTLSPGTYTLHINYTLDIPKTYQDEIFALEKSYGIEMTDRERHILALQPKNPDPTIPLKWWEAKAIFYFPKNVKILGIQGDAFNEKQFEADFAKGITYQSRISTNQTTNTISIKIQIF" }, "paralog": false, "identity": 0.9905362776025236, "score": 624.0, "evalue": 1.79e-226, "description": "Internal stop codon at 708450, 708474, 708492. Nonsense mutation around 708450, 708474, 708492.", "psc": { "uniref90_id": "UniRef90_A0A328EL65", "product": "DUF4012 domain-containing protein", "uniref50_id": "UniRef50_A0A328EL65", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EL65", "UniRef:UniRef50_A0A328EL65" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EL65", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EL65" ], "product": "DUF4012 domain-containing protein" } }, "id": "NKOCPLAKPK_1334", "locus": "GCA003260355_01299" }, { "type": "cds", "sequence": "contig_1", "start": 708561, "stop": 709151, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTAATCAGGAGCAATGGAATCAGCTTTTTCAAAAGAATATCAAGGGAATTATCTTTCTCGACTCAGAATTAATTACCTATCTTCTGCCTGCCTTTCGCTCTAAAACGCGAGAATGGCAATTTGTCAATGCTAATGTCGATCTTATTAGAGGAGAGAATAGGAGTAATAAGAAGGAGTTTTATATCAAAGATCTTCAACGCTATCTGAAAGATAATGCTCTCACGCTTGCAAAGTCTACGATCAACAACATTCAAGAGCTCCTGCATAAGGGATTTATCAATATCTACCTCTCCAATGTCTCTGATGAAATGCGAACGTTCTTGAAAGACTATGATCTGACCAATGTGTATAATCCAGACTTTTTCTACTTTTTCCATATTAATAGCTCGTTTAATAAGTCTGATGGATTTGTCAAAAAACAGGTAGAGGTGCTAGGACCTCAAGGGAACGTTCTTCTCTCTACAGAAGAAAGAAAGCTGGACATTCATACACTTTCACCGGGAACCTATACCCTCCACATTAATTATACGCTTGATATTCCCAAGACCTATCAAGATGAGATCTTTGCACTAGAGAAGAGCTACTGA", "aa": "MFNQEQWNQLFQKNIKGIIFLDSELITYLLPAFRSKTREWQFVNANVDLIRGENRSNKKEFYIKDLQRYLKDNALTLAKSTINNIQELLHKGFINIYLSNVSDEMRTFLKDYDLTNVYNPDFFYFFHINSSFNKSDGFVKKQVEVLGPQGNVLLSTEERKLDIHTLSPGTYTLHINYTLDIPKTYQDEIFALEKSY", "aa_hexdigest": "41d864e06cc8902a145b762e6f325623", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 23205.04460000001, "isoelectric_point": 6.431411552429199 }, "id": "NKOCPLAKPK_1335", "locus": "GCA003260355_01300" }, { "type": "cds", "sequence": "contig_1", "start": 709158, "stop": 709406, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACCGATAGAGAACGTCACATTCTTGCATTACAGCCAAAGAATCCTGATCCTACGATTCCTCTCAAATGGTGGGAAGCTAAAGCGATTTTCTATTTCCCAAAGAATGTAAAGATCCTTGGTATTCAAGGCGATGCTTTTAATGAAAAACAGTTTGAAGCAGATTTTGCCAAGGGGATTACCTATCAATCACGCATCTCTACCAACCAAACGACGAATACAATCAGTATCAAAATTCAGATTTTTTAA", "aa": "MTDRERHILALQPKNPDPTIPLKWWEAKAIFYFPKNVKILGIQGDAFNEKQFEADFAKGITYQSRISTNQTTNTISIKIQIF", "aa_hexdigest": "32770e801def45443d1c24849d90a3e0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9526.840399999994, "isoelectric_point": 9.397121238708493 }, "id": "NKOCPLAKPK_1336", "locus": "GCA003260355_01301" }, { "type": "cds", "sequence": "contig_1", "start": 709422, "stop": 709886, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EHH8", "UniRef:UniRef50_A0A3D0UTR2", "UniRef:UniRef90_A0A328EHH8" ], "nt": "ATGCTAAAATTACTGCAAGGCTATTACCATTTTCTCTCGCTGGGGAAGTTTATGGAACAGGTTATTTTGACAAATGACCTTTCAAGTTTGGCTATGGATCTGCCCATCAGGACCGGGGCTTCTTCCTCCTTTGGACTTAAAGAATCCTTGATTAAGCAGTTGCTCAGCTCGCTTCACAAGCATCCGGACAAGAGGAATGTTTATGGCTATTTAATTGAAATTAGTGCGTTTAAGGGGATTTTCTCTGTGATGAGAGAAATTATAGAAAACGATGCCGTCCTGAGAAACTTTCTCAAAGATCAGCTCCAAGATCAGTATTTCCCTTTTGAACAGACGATTAGATTCTTGAGAAACGTGCTTAACCATTCTACATCCTCTGGTCTAGCACTCAAACTTGAGGATTATGAAGTGCAAAGAGACTTTATCTTGTCTCCCAAAATTCAGAAAACGAACCATCTCAATTGA", "aa": "MLKLLQGYYHFLSLGKFMEQVILTNDLSSLAMDLPIRTGASSSFGLKESLIKQLLSSLHKHPDKRNVYGYLIEISAFKGIFSVMREIIENDAVLRNFLKDQLQDQYFPFEQTIRFLRNVLNHSTSSGLALKLEDYEVQRDFILSPKIQKTNHLN", "aa_hexdigest": "f1f9107ffe81745733a15109f0aa7bce", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3D0UTR2", "query_cov": 0.8506493506493507, "subject_cov": 0.8562091503267973, "identity": 0.527, "score": 151.0, "evalue": 3.86e-44, "valid": false, "uniref50_id": "UniRef50_A0A3D0UTR2" }, "pscc": { "uniref50_id": "UniRef50_A0A3D0UTR2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3D0UTR2" ] }, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 709473, "stop": 710165, "observations": { "mutation": [ 709884, 709992 ], "stop": [ 709884, 709992 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EHH8", "query_cov": 0.8896103896103896, "subject_cov": 0.5956521739130435, "identity": 1.0, "score": 269.0, "evalue": 1.4e-89, "gene_start": 18, "gene_end": 154, "reference_start": 1, "reference_end": 137, "reference_sequence": "MEQVILTNDLSSLAMDLPIRTGASSSFGLKESLIKQLLSSLHKHPDKRNVYGYLIEISAFKGIFSVMREIIENDAVLRNFLKDQLQDQYFPFEQTIRFLRNVLNHSTSSGLALKLEDYEVQRDFILSPKIQKTNHLNGAKIHLDFVYAEHIPERKGSKHYGIHLCLDFAAFKPGTPLERLVKWHDLYLLSELCFNLSQLADAKLKALKNTRKKTESPLKSRTKTIHSSRG" }, "paralog": false, "identity": 0.991304347826087, "score": 450.0, "evalue": 1.62e-163, "description": "Internal stop codon at 709884, 709992. Nonsense mutation around 709884, 709992.", "psc": { "uniref90_id": "UniRef90_A0A328EHH8", "uniref50_id": "UniRef50_A0A328EHH8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EHH8", "UniRef:UniRef50_A0A328EHH8" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EHH8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EHH8" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 17825.410999999996, "isoelectric_point": 8.804527854919435 }, "id": "NKOCPLAKPK_1337", "locus": "GCA003260355_01302" }, { "type": "cds", "sequence": "contig_1", "start": 710217, "stop": 710324, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATCTTGAAAAACTTTTTGGTAGTAAAACGAAAGTAGACATCCTCAAATATCTGATTTTCAGAAGGCAGGGCGTTTCCATGAGGGCATTAGAAACAGAAATCTGA", "aa": "MNLEKLFGSKTKVDILKYLIFRRQGVSMRALETEI", "aa_hexdigest": "3d2465185766fbee704a52e02ade2a8f", "start_type": "ATG", "rbs_motif": "TTCTT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4128.901299999999, "isoelectric_point": 9.817390632629394 }, "id": "NKOCPLAKPK_1338", "locus": "GCA003260355_01303" }, { "type": "cds", "sequence": "contig_1", "start": 710433, "stop": 710759, "strand": "+", "frame": 3, "gene": null, "product": "Nucleotidyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A9E5GNI2" ], "nt": "TTGCCTCTTTTTAAGGAGCTTTTCTTTGAGGATCTCAAGCAAAACTTGGTGACGCTCTTTGAAAAGCAACAGCACCAGATCGTTCGCTATTTTTGGGGGGACAGGTTTGGGAAGCAGATCGGAATGGACCTCATCCTGATCTATGAACAGCTCGACAAACAGCAGATCGATCAACTCAAGGTGGAAATTAGTGAAATCTTTAGACAGTTTTGGATCGAAAGTGCCTCTATTGTCTTTATGAGCCAAGAAGAACGAGAGAAAAGATACAGACTCGCCGATAGATTCGTCCTTCAAGTGATGAGATTCTACAGCTCGGTCGTGAATTAG", "aa": "MPLFKELFFEDLKQNLVTLFEKQQHQIVRYFWGDRFGKQIGMDLILIYEQLDKQQIDQLKVEISEIFRQFWIESASIVFMSQEEREKRYRLADRFVLQVMRFYSSVVN", "aa_hexdigest": "db242f7a67b687855f5c445cdcce476b", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A9E5GNI2", "query_cov": 0.8981481481481481, "subject_cov": 0.8584070796460177, "identity": 0.526, "score": 105.0, "evalue": 2.42e-27, "valid": false, "product": "Nucleotidyltransferase", "uniref50_id": "UniRef50_A0A9E5GNI2" }, "pscc": { "uniref50_id": "UniRef50_A0A9E5GNI2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A9E5GNI2" ], "product": "Nucleotidyltransferase" }, "genes": [], "id": "NKOCPLAKPK_1339", "locus": "GCA003260355_01304" }, { "type": "cds", "sequence": "contig_1", "start": 710804, "stop": 711520, "strand": "+", "frame": 2, "gene": null, "product": "AmmeMemoRadiSam system protein B", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBFAFAC", "UniRef:UniRef100_A0A328EHA6", "UniRef:UniRef50_A0A7W4ECJ2", "UniRef:UniRef90_A0A328EHA6" ], "nt": "ATGAACTACGAACAACTCCTCGATGAAATCATGGTCAATACCCTTTCAGCGAAAGAACTGATAGGGACTCCGCAAAGAGATTTCCAATATGCAGTTTGTCCGTGGGATTTGGAGTTTCATACAATGATTTTTCTCTCAAGTATTCTGCTTACTTTCGATCAGAAGAAACCGATCATTGCCCTTATTCAAGTAGCGGAGTTGCCAGAACCGGTGATGCTCTATACCGGTGAAATTGGTCCGCACTTTGGAAGGCTACGAAACGTCGATAATCAGCCTTCTCAGCTCTTTGCTCAGCATGGCTTAGCGCAGACGGAAGAAAACTTTTACCCTTACCTTGATAAACTCTTCTGCTACCTTGGCGTGATCAATCCTAACCAAGAACATCTCGTTATTTTCATCAGGCAGGGAGTCAAGCAGACCGAACTGCTTGCACTCTTGAAAGAGCTTTTGGGGAAGGACTACTCACTTTTGGTCGTGAGCAACTGCTATGACGGTTTGCCTATGCAAGCGTGCAAAGCTCAATCTGAAACGCTCGTTGCACACCTGTTGGACAAGCAGATGAGTGAAGCAGAGCAAGAAGCATTCCCAGCATTCACGATTTTGAATCAGCTCTTGCCAAAGGAACTCGGAGAATCCGCAGTGGGACAACTTGTGAATACAGGAGAACTTGGATTTAGTCAGGATTCTTCTACTTCTTTTTGGTTTATGCTAGGATAG", "aa": "MNYEQLLDEIMVNTLSAKELIGTPQRDFQYAVCPWDLEFHTMIFLSSILLTFDQKKPIIALIQVAELPEPVMLYTGEIGPHFGRLRNVDNQPSQLFAQHGLAQTEENFYPYLDKLFCYLGVINPNQEHLVIFIRQGVKQTELLALLKELLGKDYSLLVVSNCYDGLPMQACKAQSETLVAHLLDKQMSEAEQEAFPAFTILNQLLPKELGESAVGQLVNTGELGFSQDSSTSFWFMLG", "aa_hexdigest": "1e2a09d65dd9aca66e6cc8d8b9c9c95b", "start_type": "ATG", "rbs_motif": "TTCTA", "ups": { "uniparc_id": "UPI000DBFAFAC", "uniref100_id": "UniRef100_A0A328EHA6", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBFAFAC", "UniRef:UniRef100_A0A328EHA6" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EHA6", "uniref90_id": "UniRef90_A0A328EHA6" }, "psc": { "uniref90_id": "UniRef90_A0A328EHA6", "uniref50_id": "UniRef50_A0A7W4ECJ2" }, "pscc": { "uniref50_id": "UniRef50_A0A7W4ECJ2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7W4ECJ2" ], "product": "AmmeMemoRadiSam system protein B" }, "genes": [], "id": "NKOCPLAKPK_1340", "locus": "GCA003260355_01305" }, { "type": "cds", "sequence": "contig_1", "start": 711653, "stop": 712354, "strand": "+", "frame": 2, "gene": null, "product": "NodB-like y domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EGW0", "UniRef:UniRef90_A0A328EGW0" ], "nt": "GTGCTCTGTGCACCTGCTTCAAAAGCAGCCCAGGTTAGAGCCTTGACGAGAATGGTTTGGAGGGAGCAGCTCGAGAACTTCTCCCCTGAAGAGCACCGGACTAAGCAAGAGGAGCAGCAAAGCTTCACGCTCCCGATTCTTATGTTTCATTATATCCAGGATATTCCCGCAACGACGAAAGATCAGCTCGGCTACAGGCTGTCTTATGCCCCGCAGAAGCTTGATAAACTCCTTGACTTCTTTGAAGAGCACGCTATTACCCCGCTGACCTTCCGAGACCTCAAAGCTATCGTCGAGGGGAGACAAACCCCGCCCAAAAGAGCAGTAATTTTGAGCTTTGATGATGGGCATCTGGATCATTATACCAACGCTTTCCCCGTGCTGAGAAAGCATCACGCCAAGGCAGTCTTTTTTGTTATCGCGGGGAAACCTGATCAGGATCCGAGATTCGCAACTCGAGACCAGATCAGAGAGCTTGCAAGCGCAGGCTTTGAAATTGGCTCGCATTCGGTAAGCCATAGTAACCTCGCATCGCTGGGAAGCAAGCGTCTGAGGGAAGAAGTCTTTGAGTCTAAGGCGCGTATTGAAAAAGAAATCGGACTTCCCGTTCTTAGCTTCTGTTATCCAGCAGGAAGGTATGATGCCAGGGTCTTGAAACTCGTAAAAGAGGGCTATCTTTTTGCTCGCACGACCCAGCCTTGA", "aa": "MLCAPASKAAQVRALTRMVWREQLENFSPEEHRTKQEEQQSFTLPILMFHYIQDIPATTKDQLGYRLSYAPQKLDKLLDFFEEHAITPLTFRDLKAIVEGRQTPPKRAVILSFDDGHLDHYTNAFPVLRKHHAKAVFFVIAGKPDQDPRFATRDQIRELASAGFEIGSHSVSHSNLASLGSKRLREEVFESKARIEKEIGLPVLSFCYPAGRYDARVLKLVKEGYLFARTTQP", "aa_hexdigest": "eb22a34ddee92593fec464e25ac31780", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 711554, "stop": 712453, "observations": { "mutation": [ 711629, 712352 ], "stop": [ 711629, 712352 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EGW0", "query_cov": 1.0, "subject_cov": 0.7792642140468228, "identity": 0.996, "score": 461.0, "evalue": 2.77e-163, "gene_start": 1, "gene_end": 233, "reference_start": 34, "reference_end": 266, "reference_sequence": "MEKSKILLVLSACFLGIGGVLLAFWGAEGKLKAVLCAPASKAAQVRALTRMVWREQLENFSPEEHRTKQEEQQSFTLPILMFHYIQDIPATTKDQLGYRLSYAPQKLDKLLDFFEEHAITPLTFRDLKAIVEGRQTPPKRAVILSFDDGHLDHYTNAFPVLRKHHAKAVFFVIAGKPDQDPRFATRDQIRELASAGFEIGSHSVSHSNLASLGSKRLREEVFESKARIEKEIGLPVLSFCYPAGRYDARVLKLVKEGYLFARTTQPGTFLQMKRRYQLPTVRVSPTLQPEQLKRYRNLD" }, "paralog": false, "identity": 0.9933110367892977, "score": 590.0, "evalue": 3.27e-216, "description": "Internal stop codon at 711629, 712352. Nonsense mutation around 711629, 712352.", "psc": { "uniref90_id": "UniRef90_A0A328EGW0", "product": "NodB homology domain-containing protein", "uniref50_id": "UniRef50_A0A328EGW0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EGW0", "UniRef:UniRef50_A0A328EGW0" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EGW0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EGW0" ], "product": "NodB homology domain-containing protein" } }, "id": "NKOCPLAKPK_1341", "locus": "GCA003260355_01306" }, { "type": "cds", "sequence": "contig_1", "start": 712520, "stop": 713044, "strand": "+", "frame": 2, "gene": null, "product": "Nucleoside phosphorylase domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7W4E392", "UniRef:UniRef90_A0A328EJ83" ], "nt": "GTGAAACAGCAGATCAAACAGCTCCAACTCAAACAAAAGCTTTCCCTCCATTACCTCTGCACCGGGGTTGGCAACTATGCGACCATCCTTTCCTTGACGAAGTTCCTGACCGAACAGCAAGATTCAGACTTTTTTCTGATCAATCTTGGAGTCTGCGGCTACCGATCCCCTGCAAACATCGCAAAAACACCTTTCCAAGTCGCTAGAATCAAAAACTTGCACACGGGCAAGGAGCTCCTCCCTCCTCTCCCCTTCCAATTCGCTCCCGTCGCTAGCGTCCGATCCTCAGAAACTCCCCTCTTGGAACCGCTCGGCAGTGAAGGCTTCGTCGAGATGGAGGCTCGAGGCGTGGAAACGGTAGCTGATGCGTTTAGACTGCCACGCTTGTTCCTGAAAGTGCCGTTTGATAGGGTGGGAGAGGAAACCCGTGCCTTTGATAAGGAGGCGGCGTGCAAGCTCCTCGCTGAAGCGATCAATTATCGTGCACTGCTCATCCAGCTCTTCGCACGAGAGCAAAGCTTGTAA", "aa": "MKQQIKQLQLKQKLSLHYLCTGVGNYATILSLTKFLTEQQDSDFFLINLGVCGYRSPANIAKTPFQVARIKNLHTGKELLPPLPFQFAPVASVRSSETPLLEPLGSEGFVEMEARGVETVADAFRLPRLFLKVPFDRVGEETRAFDKEAACKLLAEAINYRALLIQLFAREQSL", "aa_hexdigest": "5d7429dca26bf826afbf91aa763f8327", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A328EJ83", "query_cov": 0.9942528735632183, "subject_cov": 0.9153439153439153, "identity": 1.0, "score": 336.0, "evalue": 1.69e-116, "valid": true, "product": "Nucleoside phosphorylase domain-containing protein", "uniref50_id": "UniRef50_A0A7W4E392" }, "pscc": { "uniref50_id": "UniRef50_A0A7W4E392", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7W4E392" ], "product": "Nucleoside phosphorylase domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_1342", "locus": "GCA003260355_01307" }, { "type": "cds", "sequence": "contig_1", "start": 713166, "stop": 713375, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGAAGAGCCGCTCACTTCTCAGATTTGCCTTTCAGGCTTAGCGGAGGCTTCGCTGATGCGACACTTGCATTTATCGAGGAGCAAAACGCCACCTCCAAATGTAGAGAAATGCTCTGGCATGAACATATGCTTGAGTTTTATAACGATGAGACCGTTTGCATCTTATGGTTTGAGAAAAACACGAGTCTCAGAGAAATTAGCAGCTAA", "aa": "MGRAAHFSDLPFRLSGGFADATLAFIEEQNATSKCREMLWHEHMLEFYNDETVCILWFEKNTSLREISS", "aa_hexdigest": "55fbdb1866fd80fb268483147f771445", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8029.964299999994, "isoelectric_point": 4.956553459167479 }, "id": "NKOCPLAKPK_1343", "locus": "GCA003260355_01308" }, { "type": "cds", "sequence": "contig_1", "start": 713424, "stop": 713579, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCTTCAACGATCATTTTCCCTCCTGGAAGTGGAGAAAAAGTGTTCCACGTTCCTCATAAAGAACTTTCAGATCAAACCTTTCCGTATATCAATTACCTTTCAGAGCCAGTATGGTTGTCTAATAGTAGTCTTTTCCCTGAGTATGCGGGTTGA", "aa": "MPSTIIFPPGSGEKVFHVPHKELSDQTFPYINYLSEPVWLSNSSLFPEYAG", "aa_hexdigest": "2909318ce90d71a1b6681fa60b7d3d56", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5755.420299999999, "isoelectric_point": 4.921483802795408 }, "id": "NKOCPLAKPK_1344", "locus": "GCA003260355_01309" }, { "type": "cds", "sequence": "contig_1", "start": 713938, "stop": 714327, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGAGATATGGGGGCAGGGAAAGCTTCGTTGTACAGAGCGAACATAGGCTTGTTTCCGATGGCAATCTTCCATGCCCATCCCTTTCCCTCAATACGCACCCCCTCATCACACAAAAAGAAAACTGATGCTTTGACTTTCGGACGGAAAGCGATATACTCGCAGGAGTCAGATTTTTTCTTTTTACTTGCCTTTCCCTCCTCCTTTCATCAAAAAAGGAGATACTTGGGCATCCTTTATCTTCTAAGAAAGCACAATGAAAGTCAGATTTTTTCTCTTTTGCTTAGCATTGATCATGTTGGCAGGATGTGGCCCTTCGCTAGAAGAATATTGACAGTCCTTCCCCAAAAATACGGAATCTTGAAGCGAAATAGCCTCTCCCTCCCCTGA", "aa": "MRDMGAGKASLYRANIGLFPMAIFHAHPFPSIRTPSSHKKKTDALTFGRKAIYSQESDFFFLLAFPSSFHQKRRYLGILYLLRKHNESQIFSLLLSIDHVGRMWPFARRILTVLPQKYGILKRNSLSLP", "aa_hexdigest": "4a7039a63d37e6356654b14412823663", "start_type": "ATG", "rbs_motif": "TTCTT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14924.380299999986, "isoelectric_point": 10.851141166687015 }, "id": "NKOCPLAKPK_1345", "locus": "GCA003260355_01310" }, { "type": "cds", "sequence": "contig_1", "start": 714945, "stop": 715160, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATCGCTACCAAGAAAAGTAGCCTGCCTGGATATATTCCTCTCCAGTATGCAGGGACGCAGGCAATTATCGGACTGGTAGGATATGCTGATGGCTATCTTATTGTTACACCTTTCAAGTTTCCTGTTGATAAAAGCTCCTTCAAAGCCATTCAAGACAAAACCTTTCACGGGCGAGAAAATGATATTTATGCTCCGCTAACCTATGGTCATTGA", "aa": "MIATKKSSLPGYIPLQYAGTQAIIGLVGYADGYLIVTPFKFPVDKSSFKAIQDKTFHGRENDIYAPLTYGH", "aa_hexdigest": "1e3403aa2388860bfdf447f371b6d60b", "start_type": "TTG", "rbs_motif": "TTTTT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7824.912999999999, "isoelectric_point": 8.964280509948733 }, "id": "NKOCPLAKPK_1346", "locus": "GCA003260355_01311" }, { "type": "cds", "sequence": "contig_1", "start": 715150, "stop": 715263, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTCATTGAGGGAAACGTCCTGGATTTTGGAATGGGGCACATTACTATGAAGACTATATCTGACATGATAAAGACTGGGTATATTTTCGAGACGACACCATTTCGGATTTAG", "aa": "MVIEGNVLDFGMGHITMKTISDMIKTGYIFETTPFRI", "aa_hexdigest": "4937158a02217e55fe39655af1b8918a", "start_type": "ATG", "rbs_motif": "TTTA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4207.951300000001, "isoelectric_point": 5.4648645401000975 }, "id": "NKOCPLAKPK_1347", "locus": "GCA003260355_01312" }, { "type": "cds", "sequence": "contig_1", "start": 715482, "stop": 715817, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EH96", "UniRef:UniRef90_A0A328EH96" ], "nt": "TTGCTTGCGATCTTGCTCTTGGGGCTTTCTTTTGTGGTAAAGAGGAGAGCAATAGTAGCAACCTTTAGTTTTCTTGGGGGGAAGGATGAGAACCTTTCTTTGGACTATATCCAATATTTTGCTCCAATGTTCTCTTTTGAGTATCGCTATTATGCGTTATTTTGTATCGGGACTAGTGGGCTGCTCTTTGTCGTCTATCAGATTTTTCTTTTGAAGCAAAAAGCTTTCACTCGGCGTAAATTTTTGTGGGCAACGGTGTGGTTTCTCGTAATTCTCGCAATTTGCTTTGTGATTTTATGGTTGAGTAAAATGTGGCTTGCTGAAATTTGGGAATAA", "aa": "MLAILLLGLSFVVKRRAIVATFSFLGGKDENLSLDYIQYFAPMFSFEYRYYALFCIGTSGLLFVVYQIFLLKQKAFTRRKFLWATVWFLVILAICFVILWLSKMWLAEIWE", "aa_hexdigest": "b4464fb1e705690c477141187c497301", "start_type": "TTG", "rbs_motif": "TTATT", "psc": { "uniref90_id": "UniRef90_A0A328EH96", "query_cov": 1.0, "subject_cov": 0.8951612903225806, "identity": 0.946, "score": 206.0, "evalue": 4.83e-67, "valid": true, "uniref50_id": "UniRef50_A0A328EH96" }, "pscc": { "uniref50_id": "UniRef50_A0A328EH96", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EH96" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13146.733499999991, "isoelectric_point": 9.427679252624515 }, "id": "NKOCPLAKPK_1348", "locus": "GCA003260355_01313" }, { "type": "cds", "sequence": "contig_1", "start": 716224, "stop": 716538, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTCAAAGTGGTCCCAGAAAGACCTCCGCTGGCTTCGCTATCGTCAAAGTGGTCCCAGAAAGACCTCCGCTGGCTTCGCTATCGTCAAAGTAGTCCTAGAAAGACCTCTGCTGACTTCGCTATGGGCAAAGTAGTCCCAGAAAGACCTCTGCTGGCACTGCTTACGTGCAGAGTGCGAGGGGGAAGGATCGAGCATATTGCTCGGGGGGAGGAAGAAGGGAGAGAGGGAGGAAAGATGAGAAAACTCCTGTTGAACACAGACCAAACCAGAAGAGAGTATCAGCTTTTTTATCTTTTTATACTAACACAATGA", "aa": "MVKVVPERPPLASLSSKWSQKDLRWLRYRQSSPRKTSADFAMGKVVPERPLLALLTCRVRGGRIEHIARGEEEGREGGKMRKLLLNTDQTRREYQLFYLFILTQ", "aa_hexdigest": "1d23d5d106573ae0d76c124752e9a253", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12143.040599999991, "isoelectric_point": 10.500819969177243 }, "id": "NKOCPLAKPK_1349", "locus": "GCA003260355_01314" }, { "type": "cds", "sequence": "contig_1", "start": 716535, "stop": 717329, "strand": "+", "frame": 3, "gene": null, "product": "DUF6261 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBF5F27", "UniRef:UniRef100_A0A328EKP6", "UniRef:UniRef50_A0A328EKP6", "UniRef:UniRef90_A0A328EKP6" ], "nt": "ATGACAGACTCATTAGCAATTAGACCTTTTGGGATTACCAAGCTCAATCATGCTGCCTATGGTGCGTTTATGTTAGCTTTTGAAAAGCTGATCGCCAAAGGAACAGTGGCGAAATTAGGGCTGGCAGGGACGGATTTTGATGCGTTCAAGGCGAAGCTTCAACAATTTTTAGATCTGAATCAAGAACTCGGGCAACAACAAGAGACGAAAGAACTCTTGGAGCTGGACAAAGAAAGGGATCAGCTTTTGACCTATCTCTTTGCGAAGATTGCACTGGAAGCAAGCTCACCAGAATCAACACTGAAGCAAGCAGCAGAGGTTTTGCTCCCGCTCAGGAAGCAATATCTTGGATTACAGGTGAAAGCGAACCGTGAGGAGACGTTTTTGATTGAAGGGCTGTTGGTCGACAGCAAGAAGAGTGAGTTTGCGGCTGCATTCCAAGCAGTCGGGCTCAAGAAAACGCTGGAGCGTCTGGAAGAAGTCAACAAAAGCTATCAAGAAAAGACGATGGCTCGCTCTGAAGCGAATTTAGCACAGAAAACGGTTTCAGCGAAAGCACTGCGTATGGAGATGGACGCGTTTTATCAAGCAAGCACCAAGAAGATTGATGCGGTGCAGCTGATTGCGCCTGTAGCTGAGACGGAAGCGTTCATCAACTCCTTGAATCAGCTCATTGCCGAGACGCAAGAAGCGTGGAAGCTTAGGAAATCTCAGCAAGATAGATGGACTGAGGAAGGAGAACGAGAAGATGAAGCAAATGAGAAAGAAGATGCATCTGAAGAAGCAACAGCATAA", "aa": "MTDSLAIRPFGITKLNHAAYGAFMLAFEKLIAKGTVAKLGLAGTDFDAFKAKLQQFLDLNQELGQQQETKELLELDKERDQLLTYLFAKIALEASSPESTLKQAAEVLLPLRKQYLGLQVKANREETFLIEGLLVDSKKSEFAAAFQAVGLKKTLERLEEVNKSYQEKTMARSEANLAQKTVSAKALRMEMDAFYQASTKKIDAVQLIAPVAETEAFINSLNQLIAETQEAWKLRKSQQDRWTEEGEREDEANEKEDASEEATA", "aa_hexdigest": "9f9f521740ea83cf7df944987ddc80b8", "start_type": "ATG", "rbs_motif": "TTTTT", "ups": { "uniparc_id": "UPI000DBF5F27", "uniref100_id": "UniRef100_A0A328EKP6", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBF5F27", "UniRef:UniRef100_A0A328EKP6" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EKP6", "uniref90_id": "UniRef90_A0A328EKP6" }, "psc": { "uniref90_id": "UniRef90_A0A328EKP6", "product": "Family of unknown function (DUF6261)", "uniref50_id": "UniRef50_A0A328EKP6" }, "pscc": { "uniref50_id": "UniRef50_A0A328EKP6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EKP6" ] }, "genes": [], "id": "NKOCPLAKPK_1350", "locus": "GCA003260355_01315" }, { "type": "cds", "sequence": "contig_1", "start": 717406, "stop": 717513, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACCAAAACCCGATCCAAGAAAGGAACCTATCGTATCAATGTAAAGATGGATTTCGAAGATCAGCAATCTATCGAACAGACCATGGAGTTTAGAATCAGAGAGTAG", "aa": "MTKTRSKKGTYRINVKMDFEDQQSIEQTMEFRIRE", "aa_hexdigest": "57c87ba7cfd96242266e7f28029e4ea4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4325.902399999998, "isoelectric_point": 9.39480037689209 }, "id": "NKOCPLAKPK_1351", "locus": "GCA003260355_01316" }, { "type": "cds", "sequence": "contig_1", "start": 717727, "stop": 718374, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATTTCATTACGACCGATGATAAAAAAGGCTCCTGTGAGACCGAAGCCAAGCTCTTGGAAAAATCTTCTTTCAATGTAAACTACGAAGTGCATGCTTCTTCGCCCAATGATATGACTTACAAAAAAATGGATATGGATCCGATCTTGCAAAAGAAAACCATCGCCCTCCCAGAGATCCCAACCAAACTCAAACTCAAACTCATCAAGATCGAACCTAAAACCTTCAATACCAAAGTAAACGTCTTCTTAGATGATAAACCGGTAGTCTTTACAACCGAGGGTGAATACCTCTTTGATATTACCGATGCCAAGCAACACAAAATCAAGCTCCAAATCCAAGATAAAGTGCGTGGCTTGGACTATGAAGAGAACCTTACGACGATGATCGGCTTAGATGATGTGATAGGAAAGCTCCAAGTGATTGGAGATTCCGTTGGGTTCGAACCATTTGAGGTCACGCTTGATGCTTCATCGTCCAGACTGAATGATCCTGCCGATCAGATTACCTATTTTACGTGGGACTTTGGTGATGGACAGCAGCAGCAAAAGGTTTCCAACGGTGTGATAAAGCACAGATACCGCTTTGATTACACCAAAAACAACGGAACCTTCAAACCAAAGGTCACCGTTTATACTCAGAAGTGA", "aa": "MNFITTDDKKGSCETEAKLLEKSSFNVNYEVHASSPNDMTYKKMDMDPILQKKTIALPEIPTKLKLKLIKIEPKTFNTKVNVFLDDKPVVFTTEGEYLFDITDAKQHKIKLQIQDKVRGLDYEENLTTMIGLDDVIGKLQVIGDSVGFEPFEVTLDASSSRLNDPADQITYFTWDFGDGQQQQKVSNGVIKHRYRFDYTKNNGTFKPKVTVYTQK", "aa_hexdigest": "ca6af8747f2340a0e7f7403161f417ce", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 24621.73809999999, "isoelectric_point": 5.976472282409667 }, "id": "NKOCPLAKPK_1352", "locus": "GCA003260355_01317" }, { "type": "cds", "sequence": "contig_1", "start": 718459, "stop": 719208, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCCTAATGAGAAAAATGAATTTACTCAGATTACGCAGGAGAGACCACTTCCGCTCATTGCTCCGATGAATATGACGGCACGTGTGATCCGACCCAACTTCCAAAAATTCACAAATATCGAGCTTGGTTCACAAAAGGTGACCGGGCTTCAGCTAGACTGTGGAAACGGCTCAGTCATCAACCTCAACCAAAAAACCGAATTTGAAAGACCTTGTTTCTATACCAAAAAAGGAAATTACCTTACCAGACTGATCATCCATTATGAGACCCCAAACAAAGAAAAGAAAACCGTGCCATATGCATTGAGACAAATCGTCGTTGGTTCTGAACTCGTGATCCAGGGACTCAATAAACAGCTCAGTGGAGGACAAAACGAACTCGTTGTAGGACCACTTCCAGCAGAGGTGGAGTTTAAGGCGGACAGAGTCTTTAGAGATTTCAACTTGAGCTCCTATAAGGTGCGTTGGGATGGAAATAACGACGGTAATGAAGATAAAATCGATGATGCAACCTTTAGATTCACCTATGATGAGGCAAAGGTCTACTATCCAAAGATTCGCTTCCCAGAAATCTCAGAAGAGTTGACCTATACCTTCCCGCTCAGAGTAGAACAGTCAGGAATCCCGGTCTGTAAGATCGAACTTTCTCAGCAGAAGGTAAATAATTACAATATCTCAGCGTCTTTCTTTGACGGCACAGAAAGATTTATCAGTGATTATTCTTTCGTCTTCATAGACAAAGCAACTTGA", "aa": "MPNEKNEFTQITQERPLPLIAPMNMTARVIRPNFQKFTNIELGSQKVTGLQLDCGNGSVINLNQKTEFERPCFYTKKGNYLTRLIIHYETPNKEKKTVPYALRQIVVGSELVIQGLNKQLSGGQNELVVGPLPAEVEFKADRVFRDFNLSSYKVRWDGNNDGNEDKIDDATFRFTYDEAKVYYPKIRFPEISEELTYTFPLRVEQSGIPVCKIELSQQKVNNYNISASFFDGTERFISDYSFVFIDKAT", "aa_hexdigest": "ef7c7586f198cf5568ee3530cdc72864", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 28669.156700000014, "isoelectric_point": 6.033765983581542 }, "id": "NKOCPLAKPK_1353", "locus": "GCA003260355_01318" }, { "type": "cds", "sequence": "contig_1", "start": 719326, "stop": 719475, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGCAAAGGATTTGCTCTCCGGTCTAACCCTAACGGTTATGATCGTAGTCTTGCTATTAGGAATAGTAATCCTGATTATCAACGTCTACAAGGCGTTTACCAAGAAAAATTCTTCTAAATTACGATACTACGCAGGAATCGCACTCTGA", "aa": "MAKDLLSGLTLTVMIVVLLLGIVILIINVYKAFTKKNSSKLRYYAGIAL", "aa_hexdigest": "6dc6158e195a47f16a42936fb1babfe7", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5383.626200000001, "isoelectric_point": 9.998095512390137 }, "id": "NKOCPLAKPK_1354", "locus": "GCA003260355_01319" }, { "type": "cds", "sequence": "contig_1", "start": 719506, "stop": 720075, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCCTTTTTTCAAACCTGTTGGATTTCTAGTCTAGGCAAAAATTTCCTACCGCCAGAGCCTCAAGAACTCGCCAAAAAAACCCGAGAAATTTGCAAAATTTGCCAAAAACTTTATAATAATGCTGATTTATTTCTCCTTTCAGAAAAGAACATGGCAAATAACGAAAGTTTTCTGCAACAGAATACCGCCGCAGGGGCTGAAATCTCTCATCTCAATGATGATGTAAAGATCAAGGAAATCCTCTCTGAGCAAGAGAAATTGCAGAAGCTCTATAATGAGGTCGTTGCCTATATTCAGCAGCACTCCCAGATGCCAACCGAGGAGATGATTAAATATCAGACCCAGCTCAAACAGCTGAGTGAATACTATCAGCTCAATCAACAAAAGCTCAAAACGCTGGGTTACTCCAACGTTCAGGTTAATAAGAACGTGGTCCTCAAGCAAGGCGCAAGAAAAAATATCTCGATCAAGACGATTCTCTTGGGATGTGGCTCGATTGTGTTTCTCTTTTTGCTGGGACTCATTTTTCTTTTCTCTTACCTCCTAAAGAACCCTCAGCAGCTCTGA", "aa": "MPFFQTCWISSLGKNFLPPEPQELAKKTREICKICQKLYNNADLFLLSEKNMANNESFLQQNTAAGAEISHLNDDVKIKEILSEQEKLQKLYNEVVAYIQQHSQMPTEEMIKYQTQLKQLSEYYQLNQQKLKTLGYSNVQVNKNVVLKQGARKNISIKTILLGCGSIVFLFLLGLIFLFSYLLKNPQQL", "aa_hexdigest": "9c03f9e94756b4fb31f294e2b86af7f0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 21827.159500000016, "isoelectric_point": 8.938106346130372 }, "id": "NKOCPLAKPK_1355", "locus": "GCA003260355_01320" }, { "type": "cds", "sequence": "contig_1", "start": 720115, "stop": 720507, "strand": "-", "frame": 1, "gene": null, "product": "YqgF/RNase H-like domain-containing protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBFFB40", "UniRef:UniRef100_A0A328EGW8", "UniRef:UniRef50_A0A2W4IIP0", "UniRef:UniRef90_A0A328EGW8" ], "nt": "ATGAAAATCGCACACGCACTTGGAATTGATCGAGGAAGTAAATATATCGGACTTGCTTATACTCCAATAGAAAATTCAGACATCATCTTCCCAATCGGCTATATTATGAATGATGAAATGACTTATTATTATATTGCTGATATTATCCAGAGGTATCATATTAGGAAAATCATTCTCGGTCGGCCGAGCAAACAAAAAGACATCCAAGAAAAGATCTCTAAATTTATGGCGTCGCTCAATTACATCATAGAGAACCAAAAAATTGAAATCCTGACGGTAGAGGAGGATTATACGAGCGTCCAATCAGGAGAAATCATCTCCGATTTCAAAAAGAACGCCACGACTGATACGATCAGTGCGATGCTGATCTTGGAGAGATGGAAAAAACACTAA", "aa": "MKIAHALGIDRGSKYIGLAYTPIENSDIIFPIGYIMNDEMTYYYIADIIQRYHIRKIILGRPSKQKDIQEKISKFMASLNYIIENQKIEILTVEEDYTSVQSGEIISDFKKNATTDTISAMLILERWKKH", "aa_hexdigest": "bc99f76542a6477283b810f36671a05b", "start_type": "ATG", "rbs_motif": "TTTA", "ups": { "uniparc_id": "UPI000DBFFB40", "uniref100_id": "UniRef100_A0A328EGW8", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBFFB40", "UniRef:UniRef100_A0A328EGW8" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EGW8", "uniref90_id": "UniRef90_A0A328EGW8" }, "psc": { "uniref90_id": "UniRef90_A0A328EGW8", "product": "YqgF/RNase H-like domain-containing protein", "uniref50_id": "UniRef50_A0A2W4IIP0" }, "genes": [], "id": "NKOCPLAKPK_1356", "locus": "GCA003260355_01321" }, { "type": "cds", "sequence": "contig_1", "start": 720888, "stop": 721070, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATTCTCATAAATTTTTACTAGCGTCTAAGTTATCCGGTCAGAATCTCTACTCTGTTTGGCACCTTTTAAATAATCAAGAAAGAAGAGCTATTATTAAGCAGTTATGCTCATTTTTAAAAAAAATTAACCAACTTCCTTATTATGAATTTATGGAGAAGTTCCCTTCTCCTTATGATTGA", "aa": "MYSHKFLLASKLSGQNLYSVWHLLNNQERRAIIKQLCSFLKKINQLPYYEFMEKFPSPYD", "aa_hexdigest": "7e70a05bae1c0a238bad31454a837217", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7271.400399999997, "isoelectric_point": 9.416590690612793 }, "id": "NKOCPLAKPK_1357", "locus": "GCA003260355_01322" }, { "type": "cds", "sequence": "contig_1", "start": 721215, "stop": 721535, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTTCACCGAGATATTCACTTTGATAATATCTTAGTTGAAAATATACAGATTACTGGAATGATAGATTTTGAAAAAATTATTGTTGGATCTCTTGATTTTGTTTTAAGTATGGTCAGAAGAATGTCATTATTCCCTACTAAATATATGTCAGAGGCAGATGAACCTCATGCCAAAAAAGAAGATTACGAAAATCTGCTACAACGATTTGAGGAGTACTATCCTGAGCTGTTTGACTTTAAGGATTTAGCTATTAGGTTAATGTTTTATGATCTTGAATATGATCTAAGGTTATTGAAGCATCATCCGCAGGAGCAATGA", "aa": "MVHRDIHFDNILVENIQITGMIDFEKIIVGSLDFVLSMVRRMSLFPTKYMSEADEPHAKKEDYENLLQRFEEYYPELFDFKDLAIRLMFYDLEYDLRLLKHHPQEQ", "aa_hexdigest": "f3939a7480d2a18f71c2f1c200e9800e", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12917.696699999991, "isoelectric_point": 4.872659111022949 }, "id": "NKOCPLAKPK_1358", "locus": "GCA003260355_01323" }, { "type": "cds", "sequence": "contig_1", "start": 721571, "stop": 721747, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTATGAAAGAGACTATTTTGATGAAGCACTTCCTTCTTTAGACTATACATTTGAGCAGCAGGGGCTCTCTTTGCATTTCAAAGGGGAAACCTACCATTTACCTCGAGCAGATTTTGCTCCTCAAGAGGTTGAAATGCTTCATCCAAACTATATGATCGAACCAAATGTTAACTAG", "aa": "MYERDYFDEALPSLDYTFEQQGLSLHFKGETYHLPRADFAPQEVEMLHPNYMIEPNVN", "aa_hexdigest": "b98651397107d5246749668147299424", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6938.609, "isoelectric_point": 4.396973609924316 }, "id": "NKOCPLAKPK_1359", "locus": "GCA003260355_01324" }, { "type": "cds", "sequence": "contig_1", "start": 722018, "stop": 722182, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGATAAGCATAGACATCTCTTCGGATAAGATCAGAATTTTTGGACTTGATCATCCTATTTTTCTGGAGAGAACAGGAGTTGACGTTGAGCTGGGAAAGGTCTTAGTAAACCTTGACAAAGAGAAAAACCTGACCGAAATACTGGTGCTCAACGGACCATGA", "aa": "MKISIDISSDKIRIFGLDHPIFLERTGVDVELGKVLVNLDKEKNLTEILVLNGP", "aa_hexdigest": "0f54447d7ade7e6d741c8161f4487876", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6047.0262, "isoelectric_point": 5.115475273132325 }, "id": "NKOCPLAKPK_1360", "locus": "GCA003260355_01325" }, { "type": "cds", "sequence": "contig_1", "start": 722255, "stop": 723532, "strand": "-", "frame": 3, "gene": null, "product": "GOLD domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EKE3", "UniRef:UniRef90_A0A328EKE3" ], "nt": "GTGTCTGTTCGTGTAAGAGGAAACGGATATTCCTACTCAAATTATGACAAAAATATCAGATTCCGTGTAGAGCAATACAAAAATGGTAGACGAGAAAACGCCTCTAGCTACGATTATGAATTGGACAGAACAAGTTATTCTTTCTCTTCCTATGAAAATGGAGAAAAAAGATTTGATCGCTTAGTAAAGTTTAGATCAGAAGGTCAATTCAGATTGTATGCTGAAATCGACGGTGGAAATTCCGTCTTCAGAGAGTTTAATACAACGACTTCTTCATCTTCTGAGTATTATGCTCCAGAATGGACCTCAATAAGTGATAAAAATCCAGATCAAAATAAACGAGTAGATATGACATTGAGAGTAAGAAGAAATGGGTCTGTAAATTATTATGGAGGAGTGGACTTCCGTGTAGAACAATACAAAAACGGTAGACGAGAAAATGCTTCTAGCTATGATTATCAGCTAGATAGGACAAGCTACTACTTCTCATCTACAGATAATGGAGAAGTCAGATTAAATAACTTCGTAAAATTCTCAACCAATGGGGAGTTTAGAGTTGTTGCTCAGCTTCGTGAATACGGGACAGCGACCGCTTATCAAACCTTCAATGTTAACGGTGGAACTTCTTCTAATAATTCTACATATTACAGTGTTGATAATCTTTCAATCAGAGAAATCTATCCTTCTTCTCCTCGCACAGATGAATGGGTTGATCTGACAATAAGAGCACAAGATCGCTACAATAGCAAAGTAGCAAATTACGAAGGAAGGGTAAAGCTTCTCGTAGAGGAGTACAGAAACGGATCATGGAGAACAGCAAGTAGCTCAGATTACCAGCTAGATCAGCAGTATGTAAATTTCTCTCGCTATGACAACGGGGAAAAGACCTTCTCAGATATTGTAAAGTTTAGGACAACAGGAGAATTTAGAATCAGAGCCGAGGATGATAATAGATCAGGAATCTATGGGACAAAGGAGATTCGTGTATATTCCTCTTCTTCTAATAATAATTCTCGATCCAACTCTTATTACAACTTCACTGACAAAGAGCTTTCCAAAATCAGAGCAGTATCCAACATTTGGAATGACGTTATCGTAGCTCTAGAGAGAGACTATCCAAGACTAAGAAATAGCATGCAGTGGAGAAACCTTTCTTCAGACTTCTATAGAAATATGCAAGACGTCCTAAGAAACGCTAGCTCCGCAAAATTCAGAGGATGGAGTGAATTCTATTCAGCATTCCAAGAATGGTTTAGTCTTACTATCAGGACAAGATAG", "aa": "MSVRVRGNGYSYSNYDKNIRFRVEQYKNGRRENASSYDYELDRTSYSFSSYENGEKRFDRLVKFRSEGQFRLYAEIDGGNSVFREFNTTTSSSSEYYAPEWTSISDKNPDQNKRVDMTLRVRRNGSVNYYGGVDFRVEQYKNGRRENASSYDYQLDRTSYYFSSTDNGEVRLNNFVKFSTNGEFRVVAQLREYGTATAYQTFNVNGGTSSNNSTYYSVDNLSIREIYPSSPRTDEWVDLTIRAQDRYNSKVANYEGRVKLLVEEYRNGSWRTASSSDYQLDQQYVNFSRYDNGEKTFSDIVKFRTTGEFRIRAEDDNRSGIYGTKEIRVYSSSSNNNSRSNSYYNFTDKELSKIRAVSNIWNDVIVALERDYPRLRNSMQWRNLSSDFYRNMQDVLRNASSAKFRGWSEFYSAFQEWFSLTIRTR", "aa_hexdigest": "91416519db078eac6264ff70d4ad9e98", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 722255, "stop": 723832, "observations": { "mutation": [ 723712 ], "stop": [ 723712 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EKE3", "query_cov": 1.0023529411764707, "subject_cov": 0.7383015597920277, "identity": 0.991, "score": 840.0, "evalue": 3.31e-305, "gene_start": 1, "gene_end": 425, "reference_start": 152, "reference_end": 577, "reference_sequence": "MKKRSLLLTGMLGLLAIPGAFASAACQDIQFTDGQKFCFDIQKLNADRFQARISSSNLKSLSELSCRLKLPNGNEVALPRCEGEFSYAGSDGRIELRADIQNYRYELVDNYSFRNGSFGASSSNTSPSYSNYQVEFTSTNPSNPARDQRVDVSVRVRGNGYSYSNYDKNIRFRVEQYKNGRRENASSYDYELDRTSYSFSSYENGEKRFDRLVKFRSEGQFRLYAEIDGGNSVFREFNTTTSSSSSEYYAPEWTSISDKNPDQNKRVDMTLRVRRNGSVNYYGGVDFRVEQYKNGRRENASSYDYQLDRTSYYFSSTDNGEVRLNNFVKFSTNGEFRVVAQLREYGTATAYQTFNVNGGTSSNNSTYYSVDNLSIREIYPSSPRTDEWVDLTIRAQDRYNSKVTNYEGRVKLLVEEYRNGSWRTASSSDYQLDQQYVNFSRYDNGEKTFSDIVKFRTTGEFRIRAEDDNRSGIYGTKEVRVYSSSSNNNSRSNSYYNFTDKELSKIRAVSNIWNDVIVALERDYPRLRNSMQWRNLSSDFYRNMQDVLRNASSAKFRGWSEFYSAFQEWFSLTIRTR" }, "paralog": false, "identity": 0.9904942965779467, "score": 1051.0, "evalue": 0.0, "description": "Internal stop codon at 723712. Nonsense mutation around 723712.", "psc": { "uniref90_id": "UniRef90_A0A328EKE3", "product": "GOLD domain-containing protein", "uniref50_id": "UniRef50_A0A328EKE3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EKE3", "UniRef:UniRef50_A0A328EKE3" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EKE3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EKE3" ], "product": "GOLD domain-containing protein" } }, "id": "NKOCPLAKPK_1361", "locus": "GCA003260355_01326" }, { "type": "cds", "sequence": "contig_1", "start": 724117, "stop": 724470, "strand": "+", "frame": 1, "gene": null, "product": "Phosphatidylserine decarboxylase family protein", "db_xrefs": [ "EC:4.1.1.65", "KEGG:K01613", "SO:0001217", "UniRef:UniRef50_A0A7W4E2W1", "UniRef:UniRef90_A0A660MRE5" ], "nt": "ATGAAGTGGTTAAAAATGTCGTTAGTGATCAGTCTTGTTTTTTTGATTGGTATTGGGACCTTTTATAGATTTTACTTTCTCCGCGATCCAATTCGTGCAGTTCCAAATGATGATACGATGTTTGTTAGCCCTGCTAATGGGAAAATTATCGCTATTATCAAGCAAGATGAACTAAAAGATCATAAAACAGAATTATATAAGGAAAATAAAAAAGTGATCAATGATTGGACGGAAGGTTTTTCAAGTGGGGCAACGTTGATTTCAATCATGATGACGCCGCTTGATGTGCACTACCAAAAAGCTCCCATGGGATCTAGAATGATTGATGAAGTTTATGTGGCTGGTCGCTTTTGA", "aa": "MKWLKMSLVISLVFLIGIGTFYRFYFLRDPIRAVPNDDTMFVSPANGKIIAIIKQDELKDHKTELYKENKKVINDWTEGFSSGATLISIMMTPLDVHYQKAPMGSRMIDEVYVAGRF", "aa_hexdigest": "1eb955021ca6c3aef0198a35e3932db3", "start_type": "ATG", "rbs_motif": "TTATT", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 724117, "stop": 724764, "observations": { "mutation": [ 724468, 724648 ], "stop": [ 724468, 724648 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A660MRE5", "query_cov": 1.0, "subject_cov": 0.5441860465116279, "identity": 0.9740000000000001, "score": 229.0, "evalue": 8.89e-75, "gene_start": 1, "gene_end": 117, "reference_start": 1, "reference_end": 117, "reference_sequence": "MKWLKMSLVISLVFLIGIGAFYRFYFLRDPIRAVPNDDTMFVSPANGKIIAIIKQDELKDHKAELYKENKKVINDWTEGFSSGATLISIMMTPLDVHYQKAPMGSTMIDEVYVAGRFGNAMRSNSTMNSTFQNEYHSMLFETPEAYQFRVIQIAGFVARRIVDFLNPGQGVKQGEKIGLIKLGSQVSIVLDKNFEVLAKVGDKVVDGETILAKKK" }, "paralog": false, "identity": 0.9720930232558139, "score": 410.0, "evalue": 2.21e-148, "description": "Internal stop codon at 724468, 724648. Nonsense mutation around 724468, 724648.", "psc": { "uniref90_id": "UniRef90_A0A660MRE5", "product": "Phosphatidylserine decarboxylase family protein", "ec_ids": [ "4.1.1.65" ], "uniref50_id": "UniRef50_A0A7W4E2W1", "kegg_orthology_id": "K01613", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A660MRE5", "EC:4.1.1.65", "UniRef:UniRef50_A0A7W4E2W1", "KEGG:K01613" ] }, "pscc": { "uniref50_id": "UniRef50_A0A7W4E2W1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7W4E2W1" ], "product": "Phosphatidylserine decarboxylase family protein" } }, "id": "NKOCPLAKPK_1362", "locus": "GCA003260355_01327" }, { "type": "cds", "sequence": "contig_1", "start": 724477, "stop": 724650, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGAAGCAACTCTACGATGAATTCAACCTTTCAGAATGAATATCATAGTATGCTCTTTGAGACTCCGGAAGCGTATCAATTTCGTGTAATTCAGATTGCAGGGTTCGTAGCAAGGAGGATCGTTGACTTTTTAAATCCTGGACAAGAGGTTAAACAAGGAGAAAAAATCTGA", "aa": "MRSNSTMNSTFQNEYHSMLFETPEAYQFRVIQIAGFVARRIVDFLNPGQEVKQGEKI", "aa_hexdigest": "0378def9d8af14faa45111ab2e9261cc", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6655.467700000001, "isoelectric_point": 6.541736030578614 }, "id": "NKOCPLAKPK_1363", "locus": "GCA003260355_01328" }, { "type": "cds", "sequence": "contig_1", "start": 725030, "stop": 725119, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTATCTATGGAAAAATGTAAAAATGAAAATTGTCATGGTATGATGGCAGGAACAGCAGTTGCTTTGAGTGCAATTACTCTTATTTGA", "aa": "MVSMEKCKNENCHGMMAGTAVALSAITLI", "aa_hexdigest": "032d87c58b58c9aea8d018ad51f66c37", "start_type": "ATG", "rbs_motif": "TTTA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3054.6711999999998, "isoelectric_point": 6.503994941711425 }, "id": "NKOCPLAKPK_1364", "locus": "GCA003260355_01329" }, { "type": "cds", "sequence": "contig_1", "start": 725141, "stop": 725584, "strand": "+", "frame": 2, "gene": null, "product": "Thioredoxin domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MTR0", "UniRef:UniRef90_A0A660MTR0" ], "nt": "ATGTACCAAAAAGTAAATTCTATTTACAAAGCAGCAATTGAGATGCAATATGGATCTCAAGCAAACTTTGAAGCGTATTTTGAGGCTAGAATGACGCCAGAAGTGAGACAACAAATTGATAAAGGTATTAGCGAAACTATTACACAGTTAAAATCTCAAGGAGGAGCTACTGATACTGCAAATACTACAAAAGAAGAAAATACTGCAGCACAAGATAGTTCAGCACAAGGATCTCAATCTGGAAAACTGACTCAAGAGGAAATTGCAGGGTTTATCGGTTCAACTCCTAACTTTGCAGATAAACAGGGGAATATTTTAGTTCTTTCTTATGTTGATTTTCTCTGTAGTTATTGTAAAAAACTCCACGAAGCTGAAACTTTAGAACAGCTTAGTCAGGAGAATGAAGACGTTAGCGTTGTTCTCAAGTCTATTCCTCTCTTCTGA", "aa": "MYQKVNSIYKAAIEMQYGSQANFEAYFEARMTPEVRQQIDKGISETITQLKSQGGATDTANTTKEENTAAQDSSAQGSQSGKLTQEEIAGFIGSTPNFADKQGNILVLSYVDFLCSYCKKLHEAETLEQLSQENEDVSVVLKSIPLF", "aa_hexdigest": "6e9c59852b873708b48dd622ae93ce8b", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 725030, "stop": 725887, "observations": { "mutation": [ 725117, 725582 ], "stop": [ 725117, 725582 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A660MTR0", "query_cov": 1.0, "subject_cov": 0.4867549668874172, "identity": 0.966, "score": 267.0, "evalue": 6.47e-88, "gene_start": 1, "gene_end": 147, "reference_start": 38, "reference_end": 184, "reference_sequence": "MVSMEKCKNENCHGMMAGTAVALSAITLIGGAALGTCMYQKVNSIYKAAIEMQYGSQANFEAYFEARMTPEVRQQIDKGISETITQLKSQGGAADTANTTNEGNTAAQDSSAQGSQSGQLTQEEIAGFIGSTPNFADKQGNILVLSYVDFLCSYCKKLHEAETLEQLSQENEDVSVVLKAIPLFGEQSEAGAHGAYCAAQFGGAEKYYAYVEKAFSKQATSESDVIALAKEIGLDEAQFSGCLSSADTKSAVSEIFPAASRKFEITGTPASVIINTKTGKYEVIPGAYPKSYFEEVIKKVRG" }, "paralog": false, "identity": 0.9754385964912281, "score": 541.0, "evalue": 1.99e-198, "description": "Internal stop codon at 725117, 725582. Nonsense mutation around 725117, 725582.", "psc": { "uniref90_id": "UniRef90_A0A660MTR0", "product": "Thioredoxin domain-containing protein", "uniref50_id": "UniRef50_A0A660MTR0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A660MTR0", "UniRef:UniRef50_A0A660MTR0" ] }, "pscc": { "uniref50_id": "UniRef50_A0A660MTR0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MTR0" ], "product": "Thioredoxin domain-containing protein" } }, "id": "NKOCPLAKPK_1365", "locus": "GCA003260355_01330" }, { "type": "cds", "sequence": "contig_1", "start": 725660, "stop": 725938, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01323.26" ], "nt": "GTGGAGAAAGCGTTTTCTAAGCAAGCAACAAGTGAAAGCGATGTAATTGCTCTTGCAAAGGAAATAGGACTTGATGAAGCTCAGTTTAGTGGATGTCTTAGCAGTGCTGATACTAAGAGTGCTGTGAGTGAAATCTTTCCTGCAGCCTCAAGAAAATTCGAAATTACTGGAACTCCTGCAAGTGTAATTATCAATACAAAAACAGGAAAATACGAAGTTATTCCAGGAGCTTATCCAAAGTCATACTTTGAAGAAGTAATCAAAAAAGTAAGAGGATAA", "aa": "MEKAFSKQATSESDVIALAKEIGLDEAQFSGCLSSADTKSAVSEIFPAASRKFEITGTPASVIINTKTGKYEVIPGAYPKSYFEEVIKKVRG", "aa_hexdigest": "52126dc3b3f2e280c0a16e80935b4bc9", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01323.26", "name": "DSBA", "length": 79, "aa_cov": 0.8586956521739131, "hmm_cov": 0.9113924050632911, "evalue": 2.4565246568529473e-07, "score": 27.135417938232422, "start": 7, "stop": 85 } ], "seq_stats": { "molecular_weight": 9866.105199999996, "isoelectric_point": 6.259189414978027 }, "id": "NKOCPLAKPK_1366", "locus": "GCA003260355_01331" }, { "type": "cds", "sequence": "contig_1", "start": 726181, "stop": 726624, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGCTTTTTTGAGAAGACGGTAAAGAATTTTGTAGGGTTTATAGCGAAAGTTACTGGACAGGCTGACCCAAAACAGCAAACGGCAACGCCTCCTGCTAATGGGACTTCAAGCTCCTCTCCTGCTACAGCCACTACTCCAAGCGCTGACCAGAATAACGGAGGAAGTGATATACCTAAGAAGGAAGCAAGCTTTTTCGATAAACTTGTTGCCTCGACCCAAAACTTGTTAAACAAAACTGAACAATTCGCCACCAAGGCCACAGAGAAAACAAAACAATTGACTCAATCTATCAGAGAGGCTCCTTCAAAGGTAGTAGACAAAACAAATACCGTCATCCAGAAAGGAGTGAACTTTGGGGAAAAGGTAAAAAATCAGGCAGAACAGACGATTGATAAGGGAGTTGATTTTGGGAAAAACCTACAAAGCACGATGAAAGAGTGA", "aa": "MGFFEKTVKNFVGFIAKVTGQADPKQQTATPPANGTSSSSPATATTPSADQNNGGSDIPKKEASFFDKLVASTQNLLNKTEQFATKATEKTKQLTQSIREAPSKVVDKTNTVIQKGVNFGEKVKNQAEQTIDKGVDFGKNLQSTMKE", "aa_hexdigest": "4ea550cfc8096ffec88ee242356b60af", "start_type": "ATG", "rbs_motif": "TTCTT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15788.469699999985, "isoelectric_point": 9.426132011413575 }, "id": "NKOCPLAKPK_1367", "locus": "GCA003260355_01332" }, { "type": "cds", "sequence": "contig_1", "start": 726997, "stop": 727494, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCTCTGGAGCTGTGGATTCCTCCGCTTGAACCTCAGAGACGGTCACTGTTTCTTCATGAGCTACTTCAGGAGCAGTGCTAGTTTCCTCAGGAGTAGGTGTTACTTCTTGAGGAGCAGGAGGAGTTTCCTGAGGAGTAGGTGTGGCTACTTCAGGAGCAGCTACAGTTTCCTGAGGAGTAGATGTTACTTCTTCAGGAGCAGTGCTAATTTCCTGAGTGGTAGCAATTGCTTCTTGAGGAGCAGGGGTGGTTTCCTGAGTGGTAGCAATTGCTTCTTGAGGAGCAGTGGTAGTTTCCTGAGGAGTAGGTGTGGCTACTTCAGGAGCAGTGGTAGTTTCTTGAGTGGTAGCAATTACTTCTTGAGGAGCAGGGGTGGTTTCCTGAGGAGTTGGCGCTGTTGCTTCTGCTGTTTCGGGGGTCGAAGCAGTTGGAAGTCAGGCTTCCAAATGGTCGAGAGAGGTCGTGTTCTCTGTCTCTGTAACTACGGCTTCCTGTGA", "aa": "MLWSCGFLRLNLRDGHCFFMSYFRSSASFLRSRCYFLRSRRSFLRSRCGYFRSSYSFLRSRCYFFRSSANFLSGSNCFLRSRGGFLSGSNCFLRSSGSFLRSRCGYFRSSGSFLSGSNYFLRSRGGFLRSWRCCFCCFGGRSSWKSGFQMVERGRVLCLCNYGFL", "aa_hexdigest": "8ac4965d0d2e5ae7f9a9d65e9515d784", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19269.02589999999, "isoelectric_point": 11.068077278137206 }, "id": "NKOCPLAKPK_1368", "locus": "GCA003260355_01333" }, { "type": "cds", "sequence": "contig_1", "start": 727857, "stop": 728234, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATTCTGAGGTGCAAGGTCTTGGTAAGCTGGTTTTTTTGGTTGTTTGAGAATGCGGGAAAGCTTGCAAGGGGTATTTTTTGGGGTTTGCAAGGCTTGCAAGGGATCGGAATGGTGCATTTTTGGGGTTTGCAAGACGTGCAAGTGGTCGGAATGGTGCATTTTTGGGGTTTGCACGAGCTGCAAGGGGTCGGAATGGTGCATTTTTGGGGTTTGCACGAGCTGCAAGGGGTCAGAATGGATCATTTTTGGGGTTTGCACGAGCTGCAAGTGAAGAAAAAGGGGGCTTTTTTGTCGTTTCACGGTGTGCAAACCTCCTGCGAGGGTCCTTTTTGGTCGTTTCACGGGCTGCAAACTCCCTGCGAGGACCCTTTTTGA", "aa": "MILRCKVLVSWFFWLFENAGKLARGIFWGLQGLQGIGMVHFWGLQDVQVVGMVHFWGLHELQGVGMVHFWGLHELQGVRMDHFWGLHELQVKKKGAFLSFHGVQTSCEGPFWSFHGLQTPCEDPF", "aa_hexdigest": "8dd5dedb759ce943d0b65662da6943c2", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14425.674999999996, "isoelectric_point": 6.73879680633545 }, "id": "NKOCPLAKPK_1369", "locus": "GCA003260355_01334" }, { "type": "cds", "sequence": "contig_1", "start": 728285, "stop": 728533, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBF891A", "UniRef:UniRef100_A0A328EKQ3", "UniRef:UniRef50_A0A328EKQ3", "UniRef:UniRef90_A0A328EKQ3" ], "nt": "ATGGGCTGCAAACTCCCAGCGAGGGTCCTTTTTCATCGTTTCACGGTGTGCAAACTCCTAGCGAGGGGGCTTTTTTCAAAGGGGGCGGAAGTTGCAACTCGGAAAGTAGGAGAAAGTCAGACTTTTCTCTCTTTTTACCTCTTCAAGAAAGGGTGGAAATCTTGGTGCTTTTCTTTAGAATGCGGGTGTTTTACCTGCGTTGCTTATGAAGATGTGAATGTTGCTACTGGGAATGTTTGCGATGTATAG", "aa": "MGCKLPARVLFHRFTVCKLLARGLFSKGAEVATRKVGESQTFLSFYLFKKGWKSWCFSLECGCFTCVAYEDVNVATGNVCDV", "aa_hexdigest": "0d19f7d8cf4be9f171ea57d30086a701", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000DBF891A", "uniref100_id": "UniRef100_A0A328EKQ3", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBF891A", "UniRef:UniRef100_A0A328EKQ3" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EKQ3", "uniref90_id": "UniRef90_A0A328EKQ3" }, "psc": { "uniref90_id": "UniRef90_A0A328EKQ3", "uniref50_id": "UniRef50_A0A328EKQ3" }, "pscc": { "uniref50_id": "UniRef50_A0A328EKQ3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EKQ3" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9170.709099999995, "isoelectric_point": 8.94526233673096 }, "id": "NKOCPLAKPK_1370", "locus": "GCA003260355_01335" }, { "type": "cds", "sequence": "contig_1", "start": 728502, "stop": 728861, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EFX0", "UniRef:UniRef90_A0A328EHC5" ], "nt": "ATGTTGCTACTGGGAATGTTTGCGATGTATAGCTTTCTTTTTCCTGTGCTCCCTACTGAACCTGAGACTCCTCATATATGGGAGAGTGTTGACGGACAGAAATTTACCCGGGAGGGCTACCCTGCCATTCCTGATAACACTGAAGTCCTTTGGTGGGACTGGGATGTTGACTATCCTCCTGGGACGAGGAGAGCTTTTTTTGTGAAGGGACAACTGAAGAGAGAAGTATTCTCGTGTGAGGAGCTGAGTGAATGTGAGTTTCCTCGGGGGAAGCCTCGTATGGAGTTGTGGTCGATCATTTCCCAAGGAAAAGAACTCCACTCTTTTGGAGATTCTATTTCCTTAGGTATGACTTCTTGA", "aa": "MLLLGMFAMYSFLFPVLPTEPETPHIWESVDGQKFTREGYPAIPDNTEVLWWDWDVDYPPGTRRAFFVKGQLKREVFSCEELSECEFPRGKPRMELWSIISQGKELHSFGDSISLGMTS", "aa_hexdigest": "1b72b377458990d600f32e8f1ad47d00", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 728502, "stop": 729053, "observations": { "mutation": [ 728859, 728862, 728916 ], "stop": [ 728859, 728862, 728916 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EHC5", "query_cov": 1.0, "subject_cov": 0.6502732240437158, "identity": 1.0, "score": 258.0, "evalue": 1.26e-86, "gene_start": 1, "gene_end": 119, "reference_start": 1, "reference_end": 119, "reference_sequence": "MLLLGMFAMYSFLFPVLPTEPETPHIWESVDGQKFTREGYPAIPDNTEVLWWDWDVDYPPGTRRAFFVKGQLKREVFSCEELSECEFPRGKPRMELWSIISQGKELHSFGDSISLGMTSGGNIYSHLPVERRYPIANFGGYDLRKIANNGKEYLFFVSYNVLPDMEFSSIDSAVEDRRIMAGQ" }, "paralog": false, "identity": 0.9836065573770492, "score": 374.0, "evalue": 7.67e-135, "description": "Internal stop codon at 728859, 728862, 728916. Nonsense mutation around 728859, 728862, 728916.", "psc": { "uniref90_id": "UniRef90_A0A328EHC5", "uniref50_id": "UniRef50_A0A328EFX0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EHC5", "UniRef:UniRef50_A0A328EFX0" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EFX0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EFX0" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 13796.602699999992, "isoelectric_point": 4.743691444396972 }, "id": "NKOCPLAKPK_1371", "locus": "GCA003260355_01336" }, { "type": "cds", "sequence": "contig_1", "start": 729258, "stop": 729869, "strand": "-", "frame": 2, "gene": "ung", "product": "uracil-DNA glycosylase", "db_xrefs": [ "EC:3.2.2.27", "GO:0004844", "GO:0006284", "GO:0016799", "KEGG:K03648", "SO:0001217", "UniRef:UniRef50_P39615", "UniRef:UniRef90_A0A328EGX6" ], "nt": "ATGCCAGAGCAAAAACCTATCGCGATAGAATCCTCACGGAAAGCTGTTCTGCAGGATGAATTTCAAAAACCCTATTTTGCAGAGATCAAAACCTTCCTAAAGTCCGAAATAGAGAGAGGTCAGATCGTTTATCCACAGGGGAAAGACATTTTTAATGCTTTCAATCTCACTCCTTTTGATGAGGTAAAGGTCGTTATCCTTGGGCAAGATCCTTACCATGGGGAAGGGGAGGCGCATGGACTAAGTTTTTCCGTTCCTCAAGGGGTCAGAATTCCTCCTTCTTTGAGAAATATCTTCAAAGAATTAAAGTCTGACTTAAATATCGATCCTCCTGCGAGCGGCAACCTCGAACAACGAGCAAAGCAGGGCGTCCTTCTCCTAAACGCAGGACTGACCGTCCGCAAAGACGCCCCTAATTCGCACAAAGACATCGGACGACACCACTTTACCGACGAGGTGATAAAAAAACTTTCAGACCAAAAGACCGGCCTCGTTTTCATCTTGCGAGGAGCCTTTGCCCAACAAAAAGAAACCCTGATCGATAGAGACAAACACTTTATCATCAAGTCTCCGCATCCTTCTCCATTCTCGGCAGACAGAGGCTTTTTCTGA", "aa": "MPEQKPIAIESSRKAVLQDEFQKPYFAEIKTFLKSEIERGQIVYPQGKDIFNAFNLTPFDEVKVVILGQDPYHGEGEAHGLSFSVPQGVRIPPSLRNIFKELKSDLNIDPPASGNLEQRAKQGVLLLNAGLTVRKDAPNSHKDIGRHHFTDEVIKKLSDQKTGLVFILRGAFAQQKETLIDRDKHFIIKSPHPSPFSADRGFF", "aa_hexdigest": "680fb61daaae6b7269841e3d43e79442", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A328EGX6", "query_cov": 1.0, "subject_cov": 0.8787878787878788, "identity": 1.0, "score": 410.0, "evalue": 9.24e-145, "valid": true, "gene": "ung", "product": "uracil-DNA glycosylase", "ec_ids": [ "3.2.2.27" ], "uniref50_id": "UniRef50_P39615", "kegg_orthology_id": "K03648", "go_ids": [ "GO:0004844", "GO:0006284", "GO:0016799" ] }, "pscc": { "uniref50_id": "UniRef50_P39615", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P39615" ], "product": "Uracil-DNA glycosylase" }, "genes": [ "ung" ], "id": "NKOCPLAKPK_1372", "locus": "GCA003260355_01337" }, { "type": "cds", "sequence": "contig_1", "start": 730034, "stop": 730225, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAGTCTATTCCTTTTACCGTGTTCGTCAGGACCTTACGTAACAGTTCTTCTCCTGCTCGGTGCAGAAAGCTCCGGTTTAAATTCTTTGGGAATCGCATACCTTCTGCTTTATAATCTGATTTTCATCCTTCCAATGATCGTCATCACCGTGATGGTCGGTGGCGGACGAACTTCCGCAGAAACCCTCTGA", "aa": "MSLFLLPCSSGPYVTVLLLLGAESSGLNSLGIAYLLLYNLIFILPMIVITVMVGGGRTSAETL", "aa_hexdigest": "69ab95e155576fb965fe47cee5ecdc03", "start_type": "GTG", "rbs_motif": "TTATT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6617.934399999998, "isoelectric_point": 4.52963581085205 }, "id": "NKOCPLAKPK_1373", "locus": "GCA003260355_01338" }, { "type": "cds", "sequence": "contig_1", "start": 730247, "stop": 730417, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAATATACGACAATCTTTAAGCGAGGAGTCGGGCTTCTCGGAATTTTGATTGGGCTAGCAAATTTAAAAGATTATTTCTGGTATGGAAAAGGCTTTGTTATGGAGGTTCCACTCGCATGGAGACCTAAAATGATGAAACTGATCCAAGCGGTAGTTTCTCCAGGATGA", "aa": "MQYTTIFKRGVGLLGILIGLANLKDYFWYGKGFVMEVPLAWRPKMMKLIQAVVSPG", "aa_hexdigest": "a3304c2466dd50c5c338191f27d7a7e6", "start_type": "ATG", "rbs_motif": "TTCTT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6347.667500000001, "isoelectric_point": 9.9959680557251 }, "id": "NKOCPLAKPK_1374", "locus": "GCA003260355_01339" }, { "type": "cds", "sequence": "contig_1", "start": 730530, "stop": 730865, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAGAGGATAGTCGCTAGTAAAAGAAGCATTACAGCAAAGGCACAGGGATTAATACTATCAGAAAGAGCTCCTGAAAGCAGAATTGGGATAAACTTTTTGGGCTGTGAAGCGATATTTTCCTCCGTTCACATCTTAGTAGCTCAAGAGGACAGGAGTCTGGTTTCTTTACTCGTATTTCAAGTTTGTGTGGTTCAAGTCGTTAGAGCTAGCTGCCCTGAGGTTTCTGATTCTTCTGTGTTGTTGTCCTCCACCTTTCCACCGGTCAAGAGTGTGTTTTGTGGTGAAAGTTCGGTTGGATTTGCGGAGATGCTCGATCAGGTTTCAGCTTTTTGA", "aa": "MERIVASKRSITAKAQGLILSERAPESRIGINFLGCEAIFSSVHILVAQEDRSLVSLLVFQVCVVQVVRASCPEVSDSSVLLSSTFPPVKSVFCGESSVGFAEMLDQVSAF", "aa_hexdigest": "ab7ecd720e25803cef194b46fb61ffe0", "start_type": "TTG", "rbs_motif": "TTCTT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11907.681499999995, "isoelectric_point": 5.29070987701416 }, "id": "NKOCPLAKPK_1375", "locus": "GCA003260355_01340" }, { "type": "cds", "sequence": "contig_1", "start": 730871, "stop": 731200, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCACTTTCATTATTAACATCCTTTTCTAGTGCCAATGAAAAAAATGCAACTTATCTTTACTTTTACGGTCAAGGATGTGGGCATTGTCAGACCGTAGAAAAATATTTTGAAAAAACTGATATCGATGAGAAGGTTCAGCTCCAAAAGTACGAAATATGGTTCGACGCTACAGGGCGTCAAAAGCTTGTAGAAATCCTGCCTTCAATCGGCTTATCCCTAAATGACGTCGGAACCCCTTTTCTTGTAATGATAAACGGAGAACAGAAAGAGTACCTCATGGGAAGCACCCCAATCATTGAATACTTTTCTGCTCTTGCGAACGAGTGA", "aa": "MALSLLTSFSSANEKNATYLYFYGQGCGHCQTVEKYFEKTDIDEKVQLQKYEIWFDATGRQKLVEILPSIGLSLNDVGTPFLVMINGEQKEYLMGSTPIIEYFSALANE", "aa_hexdigest": "0a8975c6f54af4bb5d45b578c66dc92e", "start_type": "ATG", "rbs_motif": "TTCTT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12334.881599999993, "isoelectric_point": 4.673779487609862 }, "id": "NKOCPLAKPK_1376", "locus": "GCA003260355_01341" }, { "type": "cds", "sequence": "contig_1", "start": 731390, "stop": 731770, "strand": "+", "frame": 2, "gene": null, "product": "Glycosyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EHR8", "UniRef:UniRef90_A0A328EHR8" ], "nt": "ATGAAACCGCTTTTGGAGAAATTTTTTACAGCGCCAGCTCAACAAGCAGTAGAATCGATACTTTCTGTTTATCAGCAGCAGAAATTTGCTGTTGTGAATTTTGTGTATTTTGCCAACATTGTTGCACAAAAGCTTTTTAAGGATAATAAGTATAAAACAGAAAAAGAGAGAGAATACAAGAAAATCCTTTTAAAATCTGATTTTTTACTACCAGATGGTATTGCACTTCAGATTTTTTACTTTTTTGCGAGACTTTTTAGGCGTATTCATACCGATCGCTACCGATTACCCAACTTAAATGGGACAGATTTTGTGCCATATTTTCTTTCTGAGGCGAAAAAGAAGTATGGAAGCCAGAGGTTATGTCTGCTCCTCTATTGA", "aa": "MKPLLEKFFTAPAQQAVESILSVYQQQKFAVVNFVYFANIVAQKLFKDNKYKTEKEREYKKILLKSDFLLPDGIALQIFYFFARLFRRIHTDRYRLPNLNGTDFVPYFLSEAKKKYGSQRLCLLLY", "aa_hexdigest": "d6cb67c285f7b43fb602b7bddbcaba25", "start_type": "ATG", "rbs_motif": "TTTA", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 731390, "stop": 732070, "observations": { "mutation": [ 731768 ], "stop": [ 731768 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EHR8", "query_cov": 1.0, "subject_cov": 0.4581818181818182, "identity": 0.992, "score": 248.0, "evalue": 3.19e-81, "gene_start": 1, "gene_end": 126, "reference_start": 1, "reference_end": 126, "reference_sequence": "MKPLLEKIFTAPAQQAVESILSVYQQQKFAVVNFVYFANIVAQKLFKDNKYKTEKEREYKKILLKSDFLLPDGIALQIFYFFARLFRRIHTDRYRLPNLNGTDFVPYFLSEAKKKYGSQRLCLLLYGTKAEYLKIVEEKLKFQGYNVIYSQDGYSDFDWARAQTALKQYQDTTNILLVARSTPQIPLQELWTSRNYQKIQQNELLVFNTGGLFDFISGKGQKRAPKFLRTLKLEWLYRVIVDPKRNFRKVLNSVAILPYLFSYVVLGKDNHKNSQ" }, "paralog": false, "identity": 0.9867256637168141, "score": 446.0, "evalue": 9.39e-162, "description": "Internal stop codon at 731768. Nonsense mutation around 731768.", "psc": { "uniref90_id": "UniRef90_A0A328EHR8", "product": "Glycosyltransferase", "uniref50_id": "UniRef50_A0A328EHR8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EHR8", "UniRef:UniRef50_A0A328EHR8" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EHR8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EHR8" ], "product": "Glycosyltransferase" } }, "id": "NKOCPLAKPK_1377", "locus": "GCA003260355_01342" }, { "type": "cds", "sequence": "contig_1", "start": 732086, "stop": 732217, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAATGGCTCTATAGAGTGATTGTTGATCCAAAAAGAAACTTTAGGAAAGTGCTCAACTCATTTGCAATTCTTCCCTACTTGTTCAGCTATGTTGTATTAGGGAAAGATAGCCATAAAAACTCTCAGTAA", "aa": "MEWLYRVIVDPKRNFRKVLNSFAILPYLFSYVVLGKDSHKNSQ", "aa_hexdigest": "2d3af6d48bd5ce97def47b45a6258ed5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5173.000600000002, "isoelectric_point": 9.869867897033693 }, "id": "NKOCPLAKPK_1378", "locus": "GCA003260355_01343" }, { "type": "cds", "sequence": "contig_1", "start": 732228, "stop": 732620, "strand": "-", "frame": 2, "gene": null, "product": "MotA/TolQ/ExbB proton channel domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EKF1", "UniRef:UniRef90_A0A328EKF1" ], "nt": "GTGTTAATCCTCCCTTTGCAAAGGTTGCTAAAAAAGAAAAAAATCAGACTTCAAAAGGAGGTTGTAGCAGCTGTCGACGAAATGATTTATCTTTTAGCGAGGACTCAGCATCTGAAGCAATTAAATCTTAAAAATTTGGGAGGAAGCCCAAATGTTGCATTGATGAAAAGTATTTTTACCAAGGGGAATTATAATTATATTCAGAGTTCTTCCCTTATCATCGAAAATATGCACAAAGTCGAAAGTCTGCTTGGAGAAAGGGTAATCCCTGGAGAAAAAGAGCAGAATTTCACCCGCCTCCTAAAACATATTCACTTTATTAGCTTCTTTGAGGGAGTATTTAGAGGAATACTTACTATCGCAACATTGGGAATTTTCGCATTGATAGGGTAG", "aa": "MLILPLQRLLKKKKIRLQKEVVAAVDEMIYLLARTQHLKQLNLKNLGGSPNVALMKSIFTKGNYNYIQSSSLIIENMHKVESLLGERVIPGEKEQNFTRLLKHIHFISFFEGVFRGILTIATLGIFALIG", "aa_hexdigest": "f389b5600e739c3c9269f71eca8e16bb", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 732228, "stop": 732761, "observations": { "mutation": [ 732683, 732740 ], "stop": [ 732683, 732740 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EKF1", "query_cov": 0.9923076923076923, "subject_cov": 0.7288135593220338, "identity": 0.977, "score": 239.0, "evalue": 6.73e-79, "gene_start": 2, "gene_end": 130, "reference_start": 49, "reference_end": 177, "reference_sequence": "MEILHQIGTKILDVSLFIFQSFKQITGLSEPYATIVFRLICSYLIIFVLILPLQRLLKKKKIRLQKEVVAAVDEMIYLLARTQHLKQLNLKNLGGSPNVALMKSIFTKGNYNYIQSSSLILENMHKVESLLGERVIPGEKEQNFTRLLKRIHFVSFFEGVFRGILTIATLGIFALIG" }, "paralog": false, "identity": 0.9717514124293786, "score": 330.0, "evalue": 4.09e-117, "description": "Internal stop codon at 732683, 732740. Nonsense mutation around 732683, 732740.", "psc": { "uniref90_id": "UniRef90_A0A328EKF1", "product": "MotA/TolQ/ExbB proton channel domain-containing protein", "uniref50_id": "UniRef50_A0A328EKF1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EKF1", "UniRef:UniRef50_A0A328EKF1" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EKF1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EKF1" ], "product": "MotA/TolQ/ExbB proton channel domain-containing protein" } }, "id": "NKOCPLAKPK_1379", "locus": "GCA003260355_01344" }, { "type": "cds", "sequence": "contig_1", "start": 732901, "stop": 733389, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAGCTTTGAAACAGCTCAAGGAAGCTCCTTTCTTGAAAGTCGAAAAACAATTTATCATGAGTTAGAAAAGCAAGATAAATCTGATCCTCTAAAACGGCTTATCCCGATCAAAAAGAATCCCTATCGAAATGCAGAAGAGGCATTTCAACGAATAATCCCACAAAATAGCAGAAAAGATACTTTGGTATTGGTAAGCTTATTGCAGGCATTAGATATCACGGTAAGAAAGGGAGAACTTCAAGAATTAGAAAATTGGATCGCTAATGGAACTGGAATCCAGGAACTAGGTGGACGAACGATCTTTCTTTCTCACGATCAAGTATTTATCATCAAGGCGAAGCCAAGGTTTTGGGAAAAAGAGTTGCATTTAGAAAAAAAAATTACTGAAGGAGGAGTGCAATCTTTCGGAAAATTTCAATTAGATATCTCACCAAAACTGATAAATGCAACGTTGAGATTTTCAAGACCGGGGGACTATTTCCACTGA", "aa": "MSFETAQGSSFLESRKTIYHELEKQDKSDPLKRLIPIKKNPYRNAEEAFQRIIPQNSRKDTLVLVSLLQALDITVRKGELQELENWIANGTGIQELGGRTIFLSHDQVFIIKAKPRFWEKELHLEKKITEGGVQSFGKFQLDISPKLINATLRFSRPGDYFH", "aa_hexdigest": "d74b01738abf2cbd1fe932f7774f2dff", "start_type": "TTG", "rbs_motif": "TTCTT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18726.247199999994, "isoelectric_point": 9.298806953430176 }, "id": "NKOCPLAKPK_1380", "locus": "GCA003260355_01345" }, { "type": "cds", "sequence": "contig_1", "start": 733753, "stop": 733929, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATCTCGCATTTTTGATGGCGAGTTTTCGGGGGAAGAAAAATCGACCGAAAGATCAGCTTTTTTTCCTTCATTGTAATCATCAAGTAAGAAAAGAAAGTTCTGACGAGCAAAAATTCTTGAGTGATTTTTTTCAGGATTGGAATTTTATAGTTTTTATCAGAGAAAAAAACTGA", "aa": "MYLAFLMASFRGKKNRPKDQLFFLHCNHQVRKESSDEQKFLSDFFQDWNFIVFIREKN", "aa_hexdigest": "33bfb291fb273e3e128cba50ff36eff6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7177.168199999996, "isoelectric_point": 9.358375740051269 }, "id": "NKOCPLAKPK_1381", "locus": "GCA003260355_01346" }, { "type": "cds", "sequence": "contig_1", "start": 734180, "stop": 734917, "strand": "-", "frame": 3, "gene": null, "product": "Gram-positive cocci surface proteins LPxTG domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EHB3", "UniRef:UniRef90_A0A328EHB3" ], "nt": "ATGGTGAACTATGTTTCTGGTGAACGTTCTCTCAACCTAAGAAACGATCTACTTGAACTTCCTGTATATTCTACCATGGATATTGAAGGAGAGGTAGAACTTGGGATTGATGCAAGTGCCGTAAACACAGGGACATCCTATTATGGAGTAGTTGTCCCAGTTGATGATAACGTTCTTTATGGAAAGTATTCAAGAGAGTTCTGTTTTAACTTTGGAAATCAGAGATTTGCTCAGGGAGAAGCTTGTGCGACCTTTGATAATCCTAAGGGAGTAAAGGTAGCAAGTACAGATAACTCATTGAATGCACTAGAGAGTTCAGATGATGTTGAACACAACGCTGCAGGAGCAGATATGAACTTGGCAGATATTTCTCACTCAGTAAAGGGAAATGTTATAACATTGACTTGGACTGCCGTTCCAGGATCTTCAAATGTTGAAATCAAGGTATATGACGAAAAAACAGCAGATTACCAAACTCTTGCAACAGTACCAATGTCTCAAGAAAAATATGAATATACCTATGAAAATACGCCTAAGGAATTAATCTTTGCTTTCATTCCAAAAGATAGTAAAGGAAAGGAAAAGAGATATAACGTTAATGTAAGAGAAGAGGAAGATGTAAAGCCTGAAATTAAAAATGTTCCAGCAACAGGACCAGTTGAAAATGTTCTAGTGATGGCTGGAATTACATTGATGCTTTATGCTGGTTATAGAGTATTCCAAAAGAGAAGAGCATAA", "aa": "MVNYVSGERSLNLRNDLLELPVYSTMDIEGEVELGIDASAVNTGTSYYGVVVPVDDNVLYGKYSREFCFNFGNQRFAQGEACATFDNPKGVKVASTDNSLNALESSDDVEHNAAGADMNLADISHSVKGNVITLTWTAVPGSSNVEIKVYDEKTADYQTLATVPMSQEKYEYTYENTPKELIFAFIPKDSKGKEKRYNVNVREEEDVKPEIKNVPATGPVENVLVMAGITLMLYAGYRVFQKRRA", "aa_hexdigest": "fadb29797ace50fb3d13cefde76b25a1", "start_type": "ATG", "rbs_motif": "TTCTT", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 734180, "stop": 735175, "observations": { "mutation": [ 734929, 735130 ], "stop": [ 734929, 735130 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EHB3", "query_cov": 1.0, "subject_cov": 0.7401812688821753, "identity": 0.988, "score": 477.0, "evalue": 1.15e-168, "gene_start": 1, "gene_end": 245, "reference_start": 87, "reference_end": 331, "reference_sequence": "MKKQLSLLALLALGAGAVSAANLDLNQVFDQNLGYGYTENQNIQALGFQDANGTKNPSKFLVKTPILKDAKDSAVGEYYVLLGDKPMVNYVSGERSLNLRNDLLELPVYSTMDIEGEVELGIDASAVNTGTSYYGVVVPVDDNVLYGKYSREFCFNFANQRFAQGEACATFDNPKGVKVASTDNSLNALESSDDVEHNAAGADMNLADISHSVKGNVITLTWTAVPGSSNVEIKVYDEKTADYQTLATVPMSQEKYEYTYENTSKELIFAFIPKDSKGKEKRYNVNVREEVDVKPEIKNVPATGPVENVLVMAGITLMLYAGYRVFQKRRA" }, "paralog": false, "identity": 0.9848942598187311, "score": 642.0, "evalue": 3.36e-236, "description": "Internal stop codon at 734929, 735130. Nonsense mutation around 734929, 735130.", "psc": { "uniref90_id": "UniRef90_A0A328EHB3", "product": "Gram-positive cocci surface proteins LPxTG domain-containing protein", "uniref50_id": "UniRef50_A0A328EHB3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EHB3", "UniRef:UniRef50_A0A328EHB3" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EHB3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EHB3" ], "product": "Gram-positive cocci surface proteins LPxTG domain-containing protein" } }, "id": "NKOCPLAKPK_1382", "locus": "GCA003260355_01347" }, { "type": "cds", "sequence": "contig_1", "start": 735513, "stop": 735950, "strand": "-", "frame": 2, "gene": null, "product": "Tr-type G domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0S8GR75", "UniRef:UniRef90_A0A2W4J2D8" ], "nt": "ATGGGACAGATTAGAAACTTTTGTATTATTGCACACATTGATCATGGAAAGTCTACACTAGCAGACAGAATGCTGGAAATCACAGGATCTGTAAGAAAAGTTGATCATTCTCAAATGTTGGACCGTATGGATATTGAACAAGAAAGAGGAATTACTATTAAACTGACTCCAGCTAGAATGCAGCGGAAGGGCTATGAACTTAATTTGATTGATACGCCAGGACACGTAGATTTTCAGTATGAAGTTTCTCGTTCACTTGCTGCAGTAGAAGGGGCAATACTTCTAATTGATGCAAGTCAAGGAATCCAAGCTCAGACACTTTCGACCCTCTATCAAGCGATGGATCAAGGATTAGACATCATTCCCGTTCTCAATAAAATTGATCTCCCAGCTGCAAATCCAGATAGAGTAGCAGGGGAGATTGAAAATGTCATTTGA", "aa": "MGQIRNFCIIAHIDHGKSTLADRMLEITGSVRKVDHSQMLDRMDIEQERGITIKLTPARMQRKGYELNLIDTPGHVDFQYEVSRSLAAVEGAILLIDASQGIQAQTLSTLYQAMDQGLDIIPVLNKIDLPAANPDRVAGEIENVI", "aa_hexdigest": "dde46a388b94a23b9cfbc944b032f315", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A350X5U2", "query_cov": 1.0275862068965518, "subject_cov": 0.8713450292397661, "identity": 0.6709999999999999, "score": 194.0, "evalue": 4.62e-61, "valid": false, "product": "Elongation factor 4", "uniref50_id": "UniRef50_A0A350X5U2" }, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 735384, "stop": 735950, "observations": { "mutation": [ 735515 ], "stop": [ 735515 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A2W4J2D8", "query_cov": 1.0, "subject_cov": 0.7552083333333334, "identity": 0.9309999999999999, "score": 268.0, "evalue": 7.72e-90, "gene_start": 1, "gene_end": 145, "reference_start": 1, "reference_end": 145, "reference_sequence": "MDQIRNFCIIAHIDHGKSTLADRMLEITETVRKLNHSQVLDRMDIEQERGITIKLTPARMQWKGYELNLIDTPGHVDFQYEVSRSLAAVEGAILLVDASQGIQAQTLSTLYQAIDQGLDIIPVLNKIDLPAANPDRVAGEIENMIGLSKDEVICVSGKTGENVDKVLDAIIERFLSPSAFKQKNPKKFGLSA" }, "paralog": false, "identity": 0.898936170212766, "score": 334.0, "evalue": 2.54e-118, "description": "Internal stop codon at 735515. Nonsense mutation around 735515.", "psc": { "uniref90_id": "UniRef90_A0A2W4J2D8", "product": "Tr-type G domain-containing protein", "uniref50_id": "UniRef50_A0A0S8GR75", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A2W4J2D8", "UniRef:UniRef50_A0A0S8GR75" ] } }, "id": "NKOCPLAKPK_1383", "locus": "GCA003260355_01348" }, { "type": "cds", "sequence": "contig_1", "start": 736049, "stop": 736285, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAATTCCAAATTCCAGACAAAGATTATATTTTTGATATTGATTTGGATTTTCGAGCTCCAGAAATGGGGATAAACAATTTTGAACAAACGATAAAAAATACTAAAAAACTGATCTCATCTGCGAAAATGGTAACGATTGCGACTTCTCCATACTTTCTTGATCAAAAGCAAGCTTTAGAGCTTATTAAGCTCTTGTGTTCGGAAAGTTTAACAGGTAATATGCGACAAAAATAA", "aa": "MQFQIPDKDYIFDIDLDFRAPEMGINNFEQTIKNTKKLISSAKMVTIATSPYFLDQKQALELIKLLCSESLTGNMRQK", "aa_hexdigest": "750c7ebb3bf9defa5da511b93750d60c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9018.391799999998, "isoelectric_point": 6.152616310119628 }, "id": "NKOCPLAKPK_1384", "locus": "GCA003260355_01349" }, { "type": "cds", "sequence": "contig_1", "start": 736667, "stop": 736780, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAGAATATGGCGGTTTTCGAGTAGAACAACAAGTAGGAAATAATGCTTTTGCCTATGAAGAAAGAAAACCAAACCCTAAATTATGGATTCCACAGCTGATTTCGGATTGA", "aa": "MLEYGGFRVEQQVGNNAFAYEERKPNPKLWIPQLISD", "aa_hexdigest": "3b09836cc372bf5fc088441e75f23a85", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4338.8526, "isoelectric_point": 5.110700798034667 }, "id": "NKOCPLAKPK_1385", "locus": "GCA003260355_01350" }, { "type": "cds", "sequence": "contig_1", "start": 736954, "stop": 737055, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATGAGATTTTTGACTCAGATGAGCAAGCCTTCAAAATTTGGTTTAGCTGTTTTGGCGCTTGTAGTGCTGATTGGTGTTTTTCAGATTACTCTTGGATGA", "aa": "MMRFLTQMSKPSKFGLAVLALVVLIGVFQITLG", "aa_hexdigest": "773368f5cf140cfc0520edb9565be03a", "start_type": "ATG", "rbs_motif": "TTATT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3610.485499999999, "isoelectric_point": 11.16600475311279 }, "id": "NKOCPLAKPK_1386", "locus": "GCA003260355_01351" }, { "type": "cds", "sequence": "contig_1", "start": 737089, "stop": 737253, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGCAGGAGAGAAAATCTTATTGGATAAAACTCATCAGTTCAATATTGAGAGACTCGATAGGGAGCTTGCGGTTAACAAAATGAATGAAGATCAAATGCTAATGATCTGGAAAAGATTACCGCTCTTTAAGCCAATGATTGATAAAAAGCTAAAAGAAGCCTGA", "aa": "MAGEKILLDKTHQFNIERLDRELAVNKMNEDQMLMIWKRLPLFKPMIDKKLKEA", "aa_hexdigest": "6e011e11b859a429e20664a02359b1c4", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6512.7549, "isoelectric_point": 9.156589698791507 }, "id": "NKOCPLAKPK_1387", "locus": "GCA003260355_01352" }, { "type": "cds", "sequence": "contig_1", "start": 737311, "stop": 737469, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01464.25" ], "nt": "GTGAGTAGTGCGGGAGCTGCGGGGCTTTGGCAATTTATGCCCTCAACTGCAAAAGCATACAATTTGAGAGTTGACGACGCTATCGATGAAAGGTATCACGCAGAAAAAGCTACTGATGCGGCAATTCAATATCTCAAAAAATCTTATGAGAAGTTTTGA", "aa": "MSSAGAAGLWQFMPSTAKAYNLRVDDAIDERYHAEKATDAAIQYLKKSYEKF", "aa_hexdigest": "d3614d2dc8962ddb60b308bedef9e7ab", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01464.25", "name": "SLT", "length": 51, "aa_cov": 0.9807692307692307, "hmm_cov": 1.0, "evalue": 3.626958382857756e-14, "score": 48.805362701416016, "start": 2, "stop": 52 } ], "seq_stats": { "molecular_weight": 5859.515200000001, "isoelectric_point": 6.532925987243652 }, "id": "NKOCPLAKPK_1388", "locus": "GCA003260355_01353" }, { "type": "cds", "sequence": "contig_1", "start": 737485, "stop": 737835, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTGCTTATAATAGAGGTTCATCTGGCATTTCTAGCGATATGGCTTATCAGTTTCAAAATAACTTCTATGATCTCTATCTGAATAATGAAACCTCAAGGTATATCTTCAGAATTATTGCTTTTAAAGAAATCTTTGAAAATCTTGGTACTTATTTTGATGTTACCAAATGGGGAAAACAATACAGTGTTCCAGAAACTACCGAAGTTCAAGTCGGAAAGACAGATAATCTTGCAGCTTGGGCAGCTAGTAAAGGATACACATATCTTGAAGTAAGAACACTTAATCCCCGAATTAGAAAAAATTCACTGCCAGAAGGTTGGTGGACCTTGAAAGTTTATAAGAGATAG", "aa": "MAAYNRGSSGISSDMAYQFQNNFYDLYLNNETSRYIFRIIAFKEIFENLGTYFDVTKWGKQYSVPETTEVQVGKTDNLAAWAASKGYTYLEVRTLNPRIRKNSLPEGWWTLKVYKR", "aa_hexdigest": "2798e5b8727f32542df3df7ab1f4611e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13607.161599999983, "isoelectric_point": 9.299516105651854 }, "id": "NKOCPLAKPK_1389", "locus": "GCA003260355_01354" }, { "type": "cds", "sequence": "contig_1", "start": 737855, "stop": 738376, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTAATAAAAAATCCAAATTTTCTAATCCTTGACGAGCCAACAAACGACTTAGACCTCTTGACGATTAGTATTTTAGAAGACTTTCTTTCGCTTTTTCAGGGATGCTTAATTATTATTTCTCACGATAGGTCATTTATGGATAGGATCGTAGATCACTTATTCATTTTCGAAGGTAAAGGAAAAATTACAGATTTCTGGGGAACATACTCAGAATGGCATGCTCTCCAAGAACAGAAAAAACCTGAGATTCTACCTCAGGTTCCTCTTACTTCTTCTGGCTTATCTTCTTCCTTTTCAGCTGAAGAATCTCTTTCCTTTGAACAAAAGCTTGAATTAACCCAGCTCATGAAGGACTTAGAAGTTTTAGAAGAAGAAAAGCTCGAGATTACCAAGCTTCTTGAGAATAAAGACTTAGCTTATGATGAAATTACTTTACTCTCAGAACATCTTGGAGAGCTGCTCAAACAAATTGAAAGAAAGGAAGCAAGACGATTTGAATTAAGTAGCCTCTCGCAATAA", "aa": "MLIKNPNFLILDEPTNDLDLLTISILEDFLSLFQGCLIIISHDRSFMDRIVDHLFIFEGKGKITDFWGTYSEWHALQEQKKPEILPQVPLTSSGLSSSFSAEESLSFEQKLELTQLMKDLEVLEEEKLEITKLLENKDLAYDEITLLSEHLGELLKQIERKEARRFELSSLSQ", "aa_hexdigest": "3667a4f4589245d90f03a69dd96c2ab4", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 20044.700400000016, "isoelectric_point": 4.568570518493654 }, "id": "NKOCPLAKPK_1390", "locus": "GCA003260355_01355" }, { "type": "cds", "sequence": "contig_1", "start": 738749, "stop": 739255, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF12848.13" ], "nt": "GTGGCTCTTGCTAAGACACTGATTGATGAGCCTGGATTTTTGATTTTAGATGAACCCACCAATCATCTTGATTTACAAATGATTGAGCGATTAGAAAAGTATCTGAAAACTGAGCATTGTACGCTATTTCTCGTGACGCACGATAGATATTTTTTGGAAAACGTGTGCGATCAGATTTTTGAATTAGAAAGAGGGAAAATTTTTTCCTATCCTGCGAAATACGAATACTTCCTCAGAAAACAAGCCGAACGTCATCAAAATGAGCAGATCGCTACAGAAAAGATGCGCCAGCTACTCAAGAGAGAGCTTGCTTGGATCCGCAAATCTCCGAGAGCAAGAGCTACAAAGCAGCATTTTAGAGAGAAAGAGTTTTATACCTTAGAGTCGAGGTATGATGACCAAAAGGAGCTTCTTCATTCGGAAAGTAATAAACTTGACCTTCCTTTGCAGGAAAGAAAATTGGGAACAAAAATTCTCAAGGTAAAACATCTAAAAAAACAATTCTGA", "aa": "MALAKTLIDEPGFLILDEPTNHLDLQMIERLEKYLKTEHCTLFLVTHDRYFLENVCDQIFELERGKIFSYPAKYEYFLRKQAERHQNEQIATEKMRQLLKRELAWIRKSPRARATKQHFREKEFYTLESRYDDQKELLHSESNKLDLPLQERKLGTKILKVKHLKKQF", "aa_hexdigest": "d8488473cfba426b6e32171716d0127d", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF12848.13", "name": "ABC_tran_Xtn", "length": 72, "aa_cov": 0.4166666666666667, "hmm_cov": 1.0, "evalue": 3.106806585525851e-10, "score": 36.501991271972656, "start": 60, "stop": 129 } ], "seq_stats": { "molecular_weight": 20410.37460000001, "isoelectric_point": 9.096569633483888 }, "id": "NKOCPLAKPK_1391", "locus": "GCA003260355_01356" }, { "type": "cds", "sequence": "contig_1", "start": 739271, "stop": 739513, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTCGATCCTGAGAAAACCGTTGCTGATGAGGTGTTTGAAACAGATCATTGTTCAGCTCAACTTATCAGAGACTACGAAGAATTGTTGCACAACCCAGCTAAAGATAGCGAGTCTTTCACTAAACTAATGGTAGACTTAGATGAACATCAGATTCGAGATTATCAATCAAAAGTAGATACGATTTTGGCAAAGCTTCAGCTACAAGACCTGCTTTCTCAGCAGATAAAAACGCTTTCTTGA", "aa": "MLDPEKTVADEVFETDHCSAQLIRDYEELLHNPAKDSESFTKLMVDLDEHQIRDYQSKVDTILAKLQLQDLLSQQIKTLS", "aa_hexdigest": "6f417f943ae2a7c692f4b06a36d3df0f", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9276.317199999996, "isoelectric_point": 4.490928459167479 }, "id": "NKOCPLAKPK_1392", "locus": "GCA003260355_01357" }, { "type": "cds", "sequence": "contig_1", "start": 739586, "stop": 739738, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00005.33" ], "nt": "ATGCGATATTTTAAAGCTGAAAATCTGACCAAAATCTATACTGACAGAGCAATTATTGATCAGCTTTCTTTTTCTATTGAAAAAGGGCAGAAAATCGCTCTTGTAGCTAAAAACGGAGGAGGAAAAACAACCTTAATCAAACTCATGCTTTGA", "aa": "MRYFKAENLTKIYTDRAIIDQLSFSIEKGQKIALVAKNGGGKTTLIKLML", "aa_hexdigest": "ce6df55e18a6dd046ec44566d758dff7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00005.33", "name": "ABC_tran", "length": 30, "aa_cov": 0.6, "hmm_cov": 1.0, "evalue": 7.997168956980266e-08, "score": 29.442337036132812, "start": 20, "stop": 49 } ], "seq_stats": { "molecular_weight": 5631.654399999999, "isoelectric_point": 9.90023250579834 }, "id": "NKOCPLAKPK_1393", "locus": "GCA003260355_01358" }, { "type": "cds", "sequence": "contig_1", "start": 739899, "stop": 740135, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00590.25" ], "nt": "ATGATGCTGTATTTTATTCCAACTCCGATCGGAAATAAGGAGGATATTACGCTTAGAGCTTTGAGACTCTTGAGAGAATTGCCTATTTTTATCTGTGAAGATACGAGAGTCACGAAAAAGCTCCTTTCTCTCTATGAAATTCCTTATGAAGGGAAACAGTTTTTTTCACTTACGAGCTTTACGAATAAAGGTAAAGTAGCATATTATCTAAATTTGATGAGAGAACATGACATCTGA", "aa": "MMLYFIPTPIGNKEDITLRALRLLRELPIFICEDTRVTKKLLSLYEIPYEGKQFFSLTSFTNKGKVAYYLNLMREHDI", "aa_hexdigest": "62c967a5a7e04eafda359458f412e04a", "start_type": "ATG", "rbs_motif": "TTTTT", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00590.25", "name": "TP_methylase", "length": 45, "aa_cov": 0.5769230769230769, "hmm_cov": 1.0, "evalue": 1.2178781960910591e-09, "score": 34.84346008300781, "start": 2, "stop": 46 } ], "seq_stats": { "molecular_weight": 9261.848199999993, "isoelectric_point": 8.787185859680175 }, "id": "NKOCPLAKPK_1394", "locus": "GCA003260355_01359" }, { "type": "cds", "sequence": "contig_1", "start": 740136, "stop": 740300, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTCTCGGATGCTGGGACGCCAGGACTTTCAGATCCCGGAAAACTCCTTATCCAACTTTGTAATGAAGAAAATTTGCCTTATACGGTATTGCCAGGTGCGAATGCACTTATTCCTGCAGTAGTAGGGGCAGGGTTTGATACTTCTGCATTTCGCTATTTATGA", "aa": "MLSDAGTPGLSDPGKLLIQLCNEENLPYTVLPGANALIPAVVGAGFDTSAFRYL", "aa_hexdigest": "c519e565b3c6cb2f7b03516e09ef22e2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5592.354800000001, "isoelectric_point": 4.104764366149902 }, "id": "NKOCPLAKPK_1395", "locus": "GCA003260355_01360" }, { "type": "cds", "sequence": "contig_1", "start": 740406, "stop": 740573, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGAACTAGATGCTCTCGGCTATCAGGGGAAAATCTGCATTGCAAGGGAAATCAGCAAACTCTTTGAACAGCATCTTACCTGTGATTTTGAAGAGCTAAAAGCAATGTTAGGCAAACATCAACTCCAAATCAAAGGAGAATTTGTAGTGGGCTTAATGCCAAAGTAG", "aa": "MELDALGYQGKICIAREISKLFEQHLTCDFEELKAMLGKHQLQIKGEFVVGLMPK", "aa_hexdigest": "94bb8889bd0566ebde55fb867001d474", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6279.397199999999, "isoelectric_point": 6.041780281066894 }, "id": "NKOCPLAKPK_1396", "locus": "GCA003260355_01361" }, { "type": "tRNA", "sequence": "contig_1", "start": 740669, "stop": 740755, "strand": "-", "gene": "trnS", "product": "tRNA-Ser(gga)", "amino_acid": "Ser", "anti_codon": "gga", "score": 73.2, "nt": "GGAAGCTTGGCTGAGTGGTCGAAAGCGCATGTTTGGAGTACATGTATACGGGCAACTGTATCTAGGGTTCGAATCCCTAAGCTTCCG", "db_xrefs": [ "SO:0000269" ], "anti_codon_pos": [ 740753, 740755 ], "id": "NKOCPLAKPK_27", "locus": "GCA003260355_01362" }, { "type": "cds", "sequence": "contig_1", "start": 741424, "stop": 742077, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02899.23" ], "nt": "GTGTTGTTTTTTCCGTATGTTCAACGAAATTTTATTCTTTTCTTTATAGTTTTCAAGCTAAAAAACATAAAAAAGCTTGATTTTTTGACTTTTTTATTCTTTAGGATAAAATACTTTACTACATACGAAGATAAATCTGCAACACTCAGTTTTTATCTTCTATTCTATTTAATGAAATTTGAAAAAAACCTAATCGACGACTGTGCTACGCATTTTTTAGCACTGGGACAAAAACAGACAACCGTTTTTAGTCTTAAGTCTCATCTCGTTCTTTTTTGGCGTTTTTGTAGGACAAAAAAAGTAGTTCGCATTGAAGAAATAACTACCCAAAATATCTATGAATATCTCGAACTCTTACGTAAGACGCCAACGCAAAAAACCTCAAGATATTATGGGAAAAACAAATTTTTATCAGAGAAAACTGTCCAATCAAAAATCATCTCCATCAAAAAATTTTTTAAGTTCCTCAATTTTAGGCACGATATTGGGCTTAGCTACTTAAAAATTGAGATACCTAAAGTTCACCTCCCTCAGATGGAATATCTAACTAAGGAGGAGTTATCTCAACTCTTTACTCATATCGAAAAAAATGAAAAAAATGAAGAAAACAGGCTCAGAAATCTCTTATTCTGCAAACTTGCTTTTATATCATGA", "aa": "MLFFPYVQRNFILFFIVFKLKNIKKLDFLTFLFFRIKYFTTYEDKSATLSFYLLFYLMKFEKNLIDDCATHFLALGQKQTTVFSLKSHLVLFWRFCRTKKVVRIEEITTQNIYEYLELLRKTPTQKTSRYYGKNKFLSEKTVQSKIISIKKFFKFLNFRHDIGLSYLKIEIPKVHLPQMEYLTKEELSQLFTHIEKNEKNEENRLRNLLFCKLAFIS", "aa_hexdigest": "667424ac317eedb450ea71e1b3e37103", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02899.23", "name": "Phage_int_SAM_1", "length": 78, "aa_cov": 0.35944700460829493, "hmm_cov": 0.8205128205128205, "evalue": 1.163317973849497e-06, "score": 25.240449905395508, "start": 79, "stop": 156 } ], "seq_stats": { "molecular_weight": 26445.972300000038, "isoelectric_point": 9.736805152893066 }, "id": "NKOCPLAKPK_1397", "locus": "GCA003260355_01363" }, { "type": "cds", "sequence": "contig_1", "start": 742289, "stop": 742528, "strand": "+", "frame": 2, "gene": null, "product": "Recombinase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EHS2", "UniRef:UniRef90_A0A328EHS2" ], "nt": "GTGTATAAGTACAGACGAGATGAAAAGAACTGTCATGCGGGCTGTGTAAAGTGTAATGTTATACTACATGGTAACTACATCGTATATACAAGACGAATGCAGAGGAAGTATGGGGAAATTCTCGTGGATGAGATGATCAATGATAGGCAAATTATGAAAATCGCTACAAGCTCACTACAAGAGATGATAGACCACTATCAATCAATGGTAGATGAACTCAAGAGGACAAAAGGATTATAA", "aa": "MYKYRRDEKNCHAGCVKCNVILHGNYIVYTRRMQRKYGEILVDEMINDRQIMKIATSSLQEMIDHYQSMVDELKRTKGL", "aa_hexdigest": "17ed108ffdb31ccb6d39a8f7fe04db71", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 742109, "stop": 742528, "observations": { "mutation": [ 742130, 742211, 742235 ], "stop": [ 742130, 742211, 742235 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EHS2", "query_cov": 1.0, "subject_cov": 0.5683453237410072, "identity": 0.987, "score": 164.0, "evalue": 8.54e-51, "gene_start": 1, "gene_end": 79, "reference_start": 61, "reference_end": 139, "reference_sequence": "MKKKKTAGPTRSKLVQKLDQVFSVYIRLLVADKDGYITCPLCGARVHWTKAHNMHFITRSVYKYRRDEKNCHAGCVKCNVILHGNYIVYTRRMQRKYGEILVDEMINDRQIMKIATSSLQEMIDHYQSMVDELKRTKGL" }, "paralog": false, "identity": 0.9784172661870504, "score": 277.0, "evalue": 1.15e-97, "description": "Internal stop codon at 742130, 742211, 742235. Nonsense mutation around 742130, 742211, 742235.", "psc": { "uniref90_id": "UniRef90_A0A328EHS2", "product": "Recombinase", "uniref50_id": "UniRef50_A0A328EHS2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EHS2", "UniRef:UniRef50_A0A328EHS2" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EHS2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EHS2" ], "product": "Recombinase" } }, "id": "NKOCPLAKPK_1398", "locus": "GCA003260355_01364" }, { "type": "cds", "sequence": "contig_1", "start": 742558, "stop": 742872, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBF9C0D", "UniRef:UniRef100_A0A328EKG1", "UniRef:UniRef50_A0A328EKG1", "UniRef:UniRef90_A0A328EKG1" ], "nt": "ATGGCACGAATTTATAAAATCATAGAAAAAAGAAACCCTCACTATGAGGAGATGAAGGAGAGGTTTAAAGACAAACCAGAACTCATTGAAGAGCTAGCTCCTAGACTGTATGGACTCGCAGAGGTATACTTTACTGCAGGAAAAGATGAAGAACTAACAGATGAGCAGATAATTGAGAGAGCTGATGGGTATACTCAGAATATTTTATCTCGCTGGTATGGAAACCCTGAGAGCCTTATAGAAGACCTGAAAATGATGGTCAAATACCTCACCAAAAGACCACTTCCGTTGAATATAGATAAACGAGAAGAATAA", "aa": "MARIYKIIEKRNPHYEEMKERFKDKPELIEELAPRLYGLAEVYFTAGKDEELTDEQIIERADGYTQNILSRWYGNPESLIEDLKMMVKYLTKRPLPLNIDKREE", "aa_hexdigest": "77ca9fdda26a4a07f3b0dd427d115e5a", "start_type": "ATG", "rbs_motif": "TTTA", "ups": { "uniparc_id": "UPI000DBF9C0D", "uniref100_id": "UniRef100_A0A328EKG1", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBF9C0D", "UniRef:UniRef100_A0A328EKG1" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EKG1", "uniref90_id": "UniRef90_A0A328EKG1" }, "psc": { "uniref90_id": "UniRef90_A0A328EKG1", "uniref50_id": "UniRef50_A0A328EKG1" }, "pscc": { "uniref50_id": "UniRef50_A0A328EKG1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EKG1" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12509.204399999986, "isoelectric_point": 5.186865043640136 }, "id": "NKOCPLAKPK_1399", "locus": "GCA003260355_01365" }, { "type": "cds", "sequence": "contig_1", "start": 743030, "stop": 743128, "strand": "+", "frame": 2, "gene": null, "product": "Phage protein (N4 Gp49/phage Sf6 gene 66) family", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EKT0", "UniRef:UniRef90_A0A328EKT0" ], "nt": "ATGAAGAAGGAGAACTACGATCAAGAAGTAGGGAATAAATTTGCCTACGAAAAAGCGATAGATAAGATACGAGAACTAGAGGGCTACAAGAATAGTTAG", "aa": "MKKENYDQEVGNKFAYEKAIDKIRELEGYKNS", "aa_hexdigest": "68f245051c00e96258fd15df8c51a272", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 742904, "stop": 743128, "observations": { "mutation": [ 742961, 743000 ], "stop": [ 742961, 743000 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EKT0", "query_cov": 1.0, "subject_cov": 0.43243243243243246, "identity": 1.0, "score": 65.9, "evalue": 1.76e-13, "gene_start": 1, "gene_end": 32, "reference_start": 43, "reference_end": 74, "reference_sequence": "MLTEKNITDQIQKVEYSKLGEKTAVCLITLKNGFEIVGTSACMKKENYDQEVGNKFAYEKAIDKIRELEGYKNS" }, "paralog": false, "identity": 0.972972972972973, "score": 142.0, "evalue": 2.54e-46, "description": "Internal stop codon at 742961, 743000. Nonsense mutation around 742961, 743000.", "psc": { "uniref90_id": "UniRef90_A0A328EKT0", "product": "Phage protein (N4 Gp49/phage Sf6 gene 66) family", "uniref50_id": "UniRef50_A0A328EKT0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EKT0", "UniRef:UniRef50_A0A328EKT0" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EKT0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EKT0" ] } }, "id": "NKOCPLAKPK_1400", "locus": "GCA003260355_01366" }, { "type": "cds", "sequence": "contig_1", "start": 743638, "stop": 743922, "strand": "+", "frame": 1, "gene": null, "product": "DUF4926 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBFB0E3", "UniRef:UniRef100_A0A328ELA3", "UniRef:UniRef50_A0A2W4IKR8", "UniRef:UniRef90_A0A328ELA3" ], "nt": "ATGACAAAGAAAGAAAAACAACGAATCCCAACGGAACTACCTAAGTTCCAATATGGAGATCGCGTAGAAGTAATCTCTGATGGAGAAATTAGAGAAGGTCAAGGCATCTTACCTTATTTTTATGCTGGAAAATTTGGAGTAGTAAAAAACATTCTCCAGTATCCTGAAAGTCAAAGCTTCGGATTTGAGGTAGAGCTAGATGACAGAAAGAGGGTCATTATGAAAGAAAAAGAGCTTAAAAAAGCTGTTTCTAATCATCCATCAGACTATTCAAGTCAAGTTTAG", "aa": "MTKKEKQRIPTELPKFQYGDRVEVISDGEIREGQGILPYFYAGKFGVVKNILQYPESQSFGFEVELDDRKRVIMKEKELKKAVSNHPSDYSSQV", "aa_hexdigest": "c47dd10a463aa0a4a2fd06f4981f7d7d", "start_type": "ATG", "rbs_motif": "TTATT", "ups": { "uniparc_id": "UPI000DBFB0E3", "uniref100_id": "UniRef100_A0A328ELA3", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBFB0E3", "UniRef:UniRef100_A0A328ELA3" ] }, "ips": { "uniref100_id": "UniRef100_A0A328ELA3", "uniref90_id": "UniRef90_A0A328ELA3" }, "psc": { "uniref90_id": "UniRef90_A0A328ELA3", "uniref50_id": "UniRef50_A0A2W4IKR8" }, "pscc": { "uniref50_id": "UniRef50_A0A2W4IKR8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4IKR8" ], "product": "DUF4926 domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_1401", "locus": "GCA003260355_01367" }, { "type": "cds", "sequence": "contig_1", "start": 743942, "stop": 744175, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBFBB28", "UniRef:UniRef100_A0A328EHM0", "UniRef:UniRef50_A0A328EHM0", "UniRef:UniRef90_A0A328EHM0" ], "nt": "GTGAAATCTCAGATCACTTGGGACAAGTTGAATAAATCAAAACCCAGAGGCAGACCAGTCTCTGAGTTTCCTCAGTGGTCAGAAAAAAAGAGTGAAGAGTATAAGAGTAAAATCATCGTGGGCAAAATAATCGAATTTGTCCAGAAAGACGGAAAGAATAAAAACACTAAGGAATACTCCTTGTTTGATTTCTATAAATTCATCGTCAAGAATATACCAAACGAATACTTTTAA", "aa": "MKSQITWDKLNKSKPRGRPVSEFPQWSEKKSEEYKSKIIVGKIIEFVQKDGKNKNTKEYSLFDFYKFIVKNIPNEYF", "aa_hexdigest": "c9d61d3098d3bb25c1fb68dc395fe8d0", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000DBFBB28", "uniref100_id": "UniRef100_A0A328EHM0", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBFBB28", "UniRef:UniRef100_A0A328EHM0" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EHM0", "uniref90_id": "UniRef90_A0A328EHM0" }, "psc": { "uniref90_id": "UniRef90_A0A328EHM0", "uniref50_id": "UniRef50_A0A328EHM0" }, "pscc": { "uniref50_id": "UniRef50_A0A328EHM0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EHM0" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9282.589399999995, "isoelectric_point": 9.663440132141115 }, "id": "NKOCPLAKPK_1402", "locus": "GCA003260355_01368" }, { "type": "cds", "sequence": "contig_1", "start": 744194, "stop": 744502, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DC04CDC", "UniRef:UniRef100_A0A328EKS1", "UniRef:UniRef50_A0A328EKS1", "UniRef:UniRef90_A0A328EKS1" ], "nt": "ATGACACCAGAATTAGAACAACTTCATTCAGATTTTCAATCTGTTTTTGGGAAGGAAGTGCCAAGCAACAAAAAAAATGATGCAGAATGGATACAAAAAAAGATCGCCGAAGCAAACGAAATCAATACTCAAGATCAAACAGATCCAGTAGCAAATTCAGAAAATACTGAGCAACAGGAAACTGTAGAACAAGAACAAGAGCAAGAGCCTGAGCTTCAGAATCAAGATGAAGAAGAATATAGCTCAGAGGAAGATACACAACTGAGCTATGAGGCAAAAAAGATTTCAAAAATAAAATATCCTCTTTAA", "aa": "MTPELEQLHSDFQSVFGKEVPSNKKNDAEWIQKKIAEANEINTQDQTDPVANSENTEQQETVEQEQEQEPELQNQDEEEYSSEEDTQLSYEAKKISKIKYPL", "aa_hexdigest": "ac2413eb0829db592b993b2bb64800a1", "start_type": "ATG", "rbs_motif": "TTATT", "ups": { "uniparc_id": "UPI000DC04CDC", "uniref100_id": "UniRef100_A0A328EKS1", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DC04CDC", "UniRef:UniRef100_A0A328EKS1" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EKS1", "uniref90_id": "UniRef90_A0A328EKS1" }, "psc": { "uniref90_id": "UniRef90_A0A328EKS1", "uniref50_id": "UniRef50_A0A328EKS1" }, "pscc": { "uniref50_id": "UniRef50_A0A328EKS1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EKS1" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11876.539200000001, "isoelectric_point": 4.161603355407714 }, "id": "NKOCPLAKPK_1403", "locus": "GCA003260355_01369" }, { "type": "cds", "sequence": "contig_1", "start": 744520, "stop": 744720, "strand": "+", "frame": 1, "gene": null, "product": "HTH psq-type domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4J1S8", "UniRef:UniRef90_A0A328EHE3" ], "nt": "ATGGCAACAAAGAGAACTCGAACAGAAAAAAAGAGACCAAGAGCAGATAGCCAAAAGAACAAAAAGGCTGTCGCTCTTGCCGTTGCCAAGAATCCAATGGCAAGCACAAGAGAACTCGCAAAGATTGCAGGTGTGAGCAAAGGAACGGTTAATAATAAGTTGGGCGAACTTAGACAAGTCAAAGATGAAGCCATATTGTGA", "aa": "MATKRTRTEKKRPRADSQKNKKAVALAVAKNPMASTRELAKIAGVSKGTVNNKLGELRQVKDEAIL", "aa_hexdigest": "c4106be0abfd15127e42bd52d876d115", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 744520, "stop": 744954, "observations": { "mutation": [ 744718, 744889 ], "stop": [ 744718, 744889 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EHE3", "query_cov": 1.0, "subject_cov": 0.4583333333333333, "identity": 1.0, "score": 116.0, "evalue": 6.09e-32, "gene_start": 1, "gene_end": 66, "reference_start": 1, "reference_end": 66, "reference_sequence": "MATKRTRTEKKRPRADSQKNKKAVALAVAKNPMASTRELAKIAGVSKGTVNNKLGELRQVKDEAILGICDKDMEIVTKAQEEILRRLNSQKELTKMKTSEISSVAEASTKRYTIFRGNATDKNGGLNNPYSELSDDELLAMIKD" }, "paralog": false, "identity": 0.9861111111111112, "score": 269.0, "evalue": 1.32e-94, "description": "Internal stop codon at 744718, 744889. Nonsense mutation around 744718, 744889.", "psc": { "uniref90_id": "UniRef90_A0A328EHE3", "product": "HTH psq-type domain-containing protein", "uniref50_id": "UniRef50_A0A2W4J1S8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EHE3", "UniRef:UniRef50_A0A2W4J1S8" ] }, "pscc": { "uniref50_id": "UniRef50_A0A2W4J1S8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4J1S8" ], "product": "HTH psq-type domain-containing protein" } }, "id": "NKOCPLAKPK_1404", "locus": "GCA003260355_01370" }, { "type": "cds", "sequence": "contig_1", "start": 744736, "stop": 744891, "strand": "+", "frame": 1, "gene": null, "product": "HTH psq-type domain-containing protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI00204BF3C0", "UniRef:UniRef100_A0A328EHE3", "UniRef:UniRef50_A0A2W4J1S8", "UniRef:UniRef90_A0A328EHE3" ], "nt": "ATGGAAATTGTTACCAAAGCTCAGGAAGAGATTTTGAGGAGGCTCAATAGCCAAAAAGAACTCACAAAAATGAAAACTTCTGAAATCTCCTCAGTAGCAGAAGCAAGCACAAAGAGATATACAATCTTTAGAGGAAATGCTACAGATAAAAATTGA", "aa": "MEIVTKAQEEILRRLNSQKELTKMKTSEISSVAEASTKRYTIFRGNATDKN", "aa_hexdigest": "7059bba94fd3430f210167a254f0c707", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00204BF3C0", "uniref100_id": "UniRef100_A0A328EHE3", "db_xrefs": [ "SO:0001217", "UniParc:UPI00204BF3C0", "UniRef:UniRef100_A0A328EHE3" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EHE3", "uniref90_id": "UniRef90_A0A328EHE3" }, "psc": { "uniref90_id": "UniRef90_A0A328EHE3", "product": "HTH psq-type domain-containing protein", "uniref50_id": "UniRef50_A0A2W4J1S8" }, "pscc": { "uniref50_id": "UniRef50_A0A2W4J1S8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4J1S8" ], "product": "HTH psq-type domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_1405", "locus": "GCA003260355_01371" }, { "type": "cds", "sequence": "contig_1", "start": 744976, "stop": 745089, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGAATTGATGAGGTAAAAAAAGAACTCGCAAGGAGAGAACTCGCAAGGAGAGATTTTTTGCACTACCTCAGATATGTATTCCCCGCTTTCCAGGATGTTGCCTATCAATGA", "aa": "MGIDEVKKELARRELARRDFLHYLRYVFPAFQDVAYQ", "aa_hexdigest": "0f8555d1a6db500a8e8c2a696d139173", "start_type": "ATG", "rbs_motif": "TTTA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4545.186400000002, "isoelectric_point": 8.1604887008667 }, "id": "NKOCPLAKPK_1406", "locus": "GCA003260355_01372" }, { "type": "cds", "sequence": "contig_1", "start": 745132, "stop": 745299, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGAGTAGCAAGAGGAGAATGCAAGAGACTGATGATTTTTTGTCCTCCAAGAATGGGAAAATCAACAGACGTTACTCAATACTTCCCGAGCTGGATGTTGGGAAATAATCCAAATCTTAACTTCATTATCTCATCATACTCTGCTGATCTAGCAAATGATTTTTGA", "aa": "MRVARGECKRLMIFCPPRMGKSTDVTQYFPSWMLGNNPNLNFIISSYSADLANDF", "aa_hexdigest": "d125225c56ba4023dc67e9383578529e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6305.2503000000015, "isoelectric_point": 8.801239967346195 }, "id": "NKOCPLAKPK_1407", "locus": "GCA003260355_01373" }, { "type": "cds", "sequence": "contig_1", "start": 745289, "stop": 745471, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTTTTGAAGAAAAACAAAAAGAATTTGTGAAAGTCAGCTTTTTAAGAATGTTTTTCCAAATTTTGAATTTGCGAAAGATAAAAGAGAGTGAGGGAATTGGGAGACTGCTCAAGGAGGAGGTTACTACTCAGTCGGAGTTGGAGGATCAATCACAGGTAAGGGATCAAATATCCTTTTAA", "aa": "MIFEEKQKEFVKVSFLRMFFQILNLRKIKESEGIGRLLKEEVTTQSELEDQSQVRDQISF", "aa_hexdigest": "b041a5739a2c7976a614fda9726fd2bf", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7225.259799999998, "isoelectric_point": 5.39205379486084 }, "id": "NKOCPLAKPK_1408", "locus": "GCA003260355_01374" }, { "type": "cds", "sequence": "contig_1", "start": 745483, "stop": 745626, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAAAGATAGAGAGCAAGCGAACTCAGCAACCATTCAGGCAAAAGTAATTGATCGATATGACTCTGTGGCATTAACCAGGCTACAAGACCAGAATTCTGCAATCATCGTGATTATGACCAGATGGAATATCAACGACCTCTGA", "aa": "MKDREQANSATIQAKVIDRYDSVALTRLQDQNSAIIVIMTRWNINDL", "aa_hexdigest": "e31fca7bcc11e55e4dfa76f480e4f915", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5420.100499999999, "isoelectric_point": 5.987612724304199 }, "id": "NKOCPLAKPK_1409", "locus": "GCA003260355_01375" }, { "type": "cds", "sequence": "contig_1", "start": 745765, "stop": 746355, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTAAAGAAAACCGATATGCACTCTATCAACAAGATCCTATCAATGCAACAAATGGGATCTTTAAGAGAGGAATTTTTCAGTATTTTAAGCTTTCGGATTTTGAGAGAGCTGATGGAATACTAAAAAAATCTGATTTAACCTGTGGAATTATGATAGATCCAGCATTCTCAAGTAGTAAGAATTCTGATGACGCCACGGTTGGACTCTACGGGAAACATAAAATCACTAAAAATCTCTATCAATTGGATCTCTATGCTGATACTTCAGCACCAAGTGCAACGATCTCAGCGACCATAAGTATGGTGAACAAAGCTAAAGCAATGGGATACAAAGTCAGCTTTATTCATTGTGAGAGCGCAAAAATCAATAGAGAGCAGACGAAATTTATTCAAGACCTCAAAAAAGAGCTTATCTATCAAGATATTCAGATTCCTTTCTATACTACCGAAGCTAAGGGTAAAAAAGAGGACAGGATCAAGCAAGAATGTGAGCCACCGATGACCGCTGGAGGATTCTTTATGAGAGGTGATGGGAATACCGAAGTCCAACTCAAACAAGAACAACAATTCCTGGATTTCCCAAACTGA", "aa": "MLKENRYALYQQDPINATNGIFKRGIFQYFKLSDFERADGILKKSDLTCGIMIDPAFSSSKNSDDATVGLYGKHKITKNLYQLDLYADTSAPSATISATISMVNKAKAMGYKVSFIHCESAKINREQTKFIQDLKKELIYQDIQIPFYTTEAKGKKEDRIKQECEPPMTAGGFFMRGDGNTEVQLKQEQQFLDFPN", "aa_hexdigest": "2f6bd427e54d31e612693c17ed3e62c3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 22272.137499999986, "isoelectric_point": 8.28852291107178 }, "id": "NKOCPLAKPK_1410", "locus": "GCA003260355_01376" }, { "type": "cds", "sequence": "contig_1", "start": 746395, "stop": 746487, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATTTTAGGAAAATTGAAAAAAGGGCTGGAGAAAGTACGGATAGATATTCTGGAGTTGTGAACGTAAATAGAGATTCAGAGCTCTACTAA", "aa": "MYFRKIEKRAGESTDRYSGVVNVNRDSELY", "aa_hexdigest": "82536d27449892beaee986a7afaf9442", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3583.9386000000004, "isoelectric_point": 8.139085197448733 }, "id": "NKOCPLAKPK_1411", "locus": "GCA003260355_01377" }, { "type": "cds", "sequence": "contig_1", "start": 746614, "stop": 747030, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTAAAAATCGAGAGAAGCTTGCAGAAAAAAAGAATGCAGAAGAAAGAATGCGAACTGAGGGGCTTAGGCTTCAAGTAATTAGAGAATATGAGATGGGGGCAAGTTATGTGAAGCCAAAAAGAGACCAATATGCAGAGAGGATCGAGAAGTGGAATCCTCAGAGGAAGAAAAAAAAGAAAATCCTGAATATCAACATGATCTCTGAGGCGATAGATGTGGCGGTTTCGGTTTCTTATTCTGATGCTCTTAGTGTCAGATTTGTGTCTCGTGATGGATTCCTCCACCAAGAACAGGCTGACAAGCTCCAGTATGTCGCCGAATTTGACTCTCAAGAAGCTCACTATCAGCAGATTAAGTACCAATCAGAGCGAGATAGACACTTTTATGGAGTATCAATCAGATTCAACGAATGA", "aa": "MIKNREKLAEKKNAEERMRTEGLRLQVIREYEMGASYVKPKRDQYAERIEKWNPQRKKKKKILNINMISEAIDVAVSVSYSDALSVRFVSRDGFLHQEQADKLQYVAEFDSQEAHYQQIKYQSERDRHFYGVSIRFNE", "aa_hexdigest": "56960f418b1cab8d751ead2ec31749ea", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16522.529499999997, "isoelectric_point": 9.343805885314943 }, "id": "NKOCPLAKPK_1412", "locus": "GCA003260355_01378" }, { "type": "cds", "sequence": "contig_1", "start": 747307, "stop": 748596, "strand": "+", "frame": 1, "gene": null, "product": "Phage SU10 portal protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4KG02", "UniRef:UniRef90_A0A328EJD2" ], "nt": "GTGAATGTAGATACCTTAACAGGGAATTTCAGTCTAGGGTTGTATTATCACTTTACAATTTATGAGGGCAAGAAAGTCTTCACAGTGCGAGATGCAGAACGCAAGACTTTGCTTAGATATGTAGAACTCCAAGCTGTGACTAAGGAGGAAAAGAAGAATGAAAGGCTGATACCTCGACCAATCACCTTGACGTACTTTAAGCCTCGTAGGGGTGATGCATTCGGAGAGAGCTTCTGTGATTTGCTTGAGGATAAGCAAAACGGAAAATCAATCCTTGCGAATCTCTCAATAATTAAGGCGCAGAAAGAAGCCAGAGGAGGAAAGTTTATCGTGAACAGTAGGCTGATCACAAACAAAGAAGCAGTTCTGAATTCTTCTCCTTATGAAAGCTATATTTTCACAACACAAGACACAGAGAATGAGAATCTGAGCAATGCGATGTTTGAGCTACCTCAGAGTCAGATCAAATCTGATGTATGGTCAATGCTGAACTTTATTGACAATGAGGCTAAAAGCGCAACCGCTCAAGATGAGCTCCAGAGAGGAATCATCCCTGACAAGAGCATGACGAAAGCTGAAGCTCAGCAAGCTCAAGCGAATAACAATATCAGATCTCTCCTGAAAAATACCGTAGCTTCACGAGGAGAAAAAGACTTTTGGTTTCTTTGGTGGAGGTGCTATCTGCAATACTTCGCACAATCAGATGATAAGTTTATAGCACTGAATAACAATTTTGAGTATGCAGGAGAGACAATCAAAAAAGATGAATTCTTAACAGGTCTCGATCCTCATATTATGATTGGAACAAGGTCAGAGCTTGATTCTGTCGATGACCAGCAAGCTCAATTCTTCACCTTGCAAGTGCTCCCAATGCTCCAGAATCCTATGCTCTCTGAGGCAAGTAAAAAAATGATGATGAGGCACTGGCTAAAGCTCAATAAGATGAAACCAAATCAGATTTATGCTTTTATTCCCTTAGATGCCACAGAAAGGAAATGTAAGGCTTATGTAGAGATGGTAAACGCTGATATTATGCCTGAGTCAATCTTTGCTGATCCTAGCGTAGATTATCAAACCCTTTGGATCTATATGCAGAATGCAAAAAGTGGAGAAATGAAAGAAGCTGTCCTTGGAGCATTATCGCAAATACTGATCCAATCAGGGACTATGCAAACTACTGAGCAATACGGAGCAATAGCGAATAGTGCAAGCAATATCGCACTCGCTCAAGGAATGCAAGGAATGAGGCAAGGACTCCAAACTAGACAAGATGTTTTACCTGCTAAATAA", "aa": "MNVDTLTGNFSLGLYYHFTIYEGKKVFTVRDAERKTLLRYVELQAVTKEEKKNERLIPRPITLTYFKPRRGDAFGESFCDLLEDKQNGKSILANLSIIKAQKEARGGKFIVNSRLITNKEAVLNSSPYESYIFTTQDTENENLSNAMFELPQSQIKSDVWSMLNFIDNEAKSATAQDELQRGIIPDKSMTKAEAQQAQANNNIRSLLKNTVASRGEKDFWFLWWRCYLQYFAQSDDKFIALNNNFEYAGETIKKDEFLTGLDPHIMIGTRSELDSVDDQQAQFFTLQVLPMLQNPMLSEASKKMMMRHWLKLNKMKPNQIYAFIPLDATERKCKAYVEMVNADIMPESIFADPSVDYQTLWIYMQNAKSGEMKEAVLGALSQILIQSGTMQTTEQYGAIANSASNIALAQGMQGMRQGLQTRQDVLPAK", "aa_hexdigest": "63c43a2cb7d30aefd31bdccf0dada202", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 747007, "stop": 748596, "observations": { "mutation": [ 747028, 747145, 747181 ], "stop": [ 747028, 747145, 747181 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EJD2", "query_cov": 1.0, "subject_cov": 0.65, "identity": 0.97, "score": 814.0, "evalue": 1.81e-293, "gene_start": 1, "gene_end": 429, "reference_start": 232, "reference_end": 660, "reference_sequence": "MIKNREKLAEKKNAEERMRTEGLRLQVIREYEMGASYVKPKRDQYAERIENWNPQRKKKKKILNINMISEAIDVAVSVSYSDALSVRFVSRDGFLHQEQADKLQYVAEFDSQEAHYQQIKYQSERDRHFYGVSIRFNEGREDTKKHPIFTVIDPMTWIPDPLPSQTGKFNGQNYRFHGFSMQTSIRDMIGAKKADGSSLYKKSDLNNLIANYTKEQRESSRARNSAWNYNQVNVDTLTGNFSLGLYYHFTIYEGKKVFTVRDAERKTLLRYVELQAVTKEEKKNERLIPRPITLTYFKPRRGDAFGESFCDLLEDKQNGKSILANLSIIKAQKEARGGKFIVNSRLITNKEAVLNSSPYESYIFTTQDTENENLSNAMFELPQSQIKSDVWSMLNFIENEAKSATAQDEIQRGIIPDKSMTKAEAQQAQANNNIRSLLKNTVASRGEKDFWFLRWRCYLQYFAQSDDKFIALNNNFEYAGETIKKDEFLTGLDPHIMIGTRSELDSIDDQQAQFFTVQVLPMLQNPMISEASKKMMMRHWLKLNKMKPNQIYAFIPLDATERKCKSFVEMVNADIMPESIFADPSVDYQTLWIYMQNAKSGEMKEAVLGALSQVLIQSGTMQTTEQYGAVANSASNIALAQGMQGMGQGLQTREDVLPAK" }, "paralog": false, "identity": 0.9659735349716446, "score": 1021.0, "evalue": 0.0, "description": "Internal stop codon at 747028, 747145, 747181. Nonsense mutation around 747028, 747145, 747181.", "psc": { "uniref90_id": "UniRef90_A0A328EJD2", "product": "Phage SU10 portal protein", "uniref50_id": "UniRef50_A0A2W4KG02", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EJD2", "UniRef:UniRef50_A0A2W4KG02" ] }, "pscc": { "uniref50_id": "UniRef50_A0A2W4KG02", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4KG02" ], "product": "Portal protein" } }, "id": "NKOCPLAKPK_1413", "locus": "GCA003260355_01379" }, { "type": "cds", "sequence": "contig_1", "start": 748725, "stop": 748916, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGCTTACATTGTGAGCTACACAAATGCCAGATTGTTCTAATAGTAGCTTTCAAACTCTTTTTCTATCTCTTCGTTCTTCTTGGCGACGAAGAGTTCGCCATTGAGATTAGCACTGATTTCCTCAGGCTTTTTTATGATAAAGTTCATGAGCTTAATGACTTCTTTGAGCGCTTCATCATTACCCTCTGA", "aa": "MGLHCELHKCQIVLIVAFKLFFYLFVLLGDEEFAIEISTDFLRLFYDKVHELNDFFERFIITL", "aa_hexdigest": "ff6b131097d6e00afd3223830aa4ad34", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7604.899799999996, "isoelectric_point": 4.870158195495605 }, "id": "NKOCPLAKPK_1414", "locus": "GCA003260355_01380" }, { "type": "cds", "sequence": "contig_1", "start": 748994, "stop": 749143, "strand": "+", "frame": 2, "gene": null, "product": "Scaffolding protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI00205D1061", "UniRef:UniRef100_A0A328EKH1", "UniRef:UniRef50_A0A839JU50", "UniRef:UniRef90_A0A328EKH1" ], "nt": "ATGGATGAAAAAGAATTGCATGATCAGGAAATGGATCAAGAAATGGAAAATGAAGATCAGGAACAGGATGAGCCTGATTACAAGGCTTTATCTGAGCAGTATAAGGCACAAGCCGAGAAAGAAAAAGCTCGTGCTGATAAATACGAGTGA", "aa": "MDEKELHDQEMDQEMENEDQEQDEPDYKALSEQYKAQAEKEKARADKYE", "aa_hexdigest": "11dbedb1b6be4bfcd43abc6e675ab8c1", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00205D1061", "uniref100_id": "UniRef100_A0A328EKH1", "db_xrefs": [ "SO:0001217", "UniParc:UPI00205D1061", "UniRef:UniRef100_A0A328EKH1" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EKH1", "uniref90_id": "UniRef90_A0A328EKH1" }, "psc": { "uniref90_id": "UniRef90_A0A328EKH1", "product": "Scaffolding protein", "uniref50_id": "UniRef50_A0A839JU50" }, "pscc": { "uniref50_id": "UniRef50_A0A839JU50", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A839JU50" ], "product": "Scaffolding protein" }, "genes": [], "id": "NKOCPLAKPK_1415", "locus": "GCA003260355_01381" }, { "type": "cds", "sequence": "contig_1", "start": 749324, "stop": 749506, "strand": "+", "frame": 2, "gene": null, "product": "Scaffolding protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI002065AF56", "UniRef:UniRef100_A0A839JU50", "UniRef:UniRef50_A0A839JU50", "UniRef:UniRef90_A0A328EKH1" ], "nt": "ATGAAGCTTGTATTAGCTCAAAAAGCACCAGAGAAGTTGGTTGATCCAGCATATTTCAACAAAAAAGATGCTGGTTCTACTTCTATGCAAGGGACTACCCCAACTTCTAAGGCTCCAACAGTTGAGAATATCCTTAAGGCAAAAAGCGTTAAGGAACTCGACGAAATGGTAGGGATTGCATAA", "aa": "MKLVLAQKAPEKLVDPAYFNKKDAGSTSMQGTTPTSKAPTVENILKAKSVKELDEMVGIA", "aa_hexdigest": "69e3afa9fd47c5ec05f76f5ca2adb729", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI002065AF56", "uniref100_id": "UniRef100_A0A839JU50", "db_xrefs": [ "SO:0001217", "UniParc:UPI002065AF56", "UniRef:UniRef100_A0A839JU50" ] }, "ips": { "uniref100_id": "UniRef100_A0A839JU50", "uniref90_id": "UniRef90_A0A328EKH1" }, "psc": { "uniref90_id": "UniRef90_A0A328EKH1", "product": "Scaffolding protein", "uniref50_id": "UniRef50_A0A839JU50" }, "pscc": { "uniref50_id": "UniRef50_A0A839JU50", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A839JU50" ], "product": "Scaffolding protein" }, "genes": [], "id": "NKOCPLAKPK_1416", "locus": "GCA003260355_01382" }, { "type": "cds", "sequence": "contig_1", "start": 749532, "stop": 750674, "strand": "+", "frame": 3, "gene": null, "product": "N4-gp56 family major capsid protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBFD20B", "UniRef:UniRef100_A0A328EKT8", "UniRef:UniRef50_A0A839JT82", "UniRef:UniRef90_A0A839JT82" ], "nt": "ATGGCAGGAACTAACGACGTACAAGCATTTATTCCATCAATTTGGTCAAAAAGGACTCAATTGATGAAAAGAGAGAAATCAATTATTTACGCTATTGCAAACTTTGAGGAAAGAGCAGGTTTGCAATTTGGAGCAGAGGTATTTAGACCTTTTATGATGGATGATCTCGAGATCAATCAATACACAAGAGGTAAGGATGGGAAAGAGCAGACACTCAAGACTATGCCTGAAAAACTCTTGATTGATCAAGAGAACGAAATCACAGTGTATATTGATACCAACGATCAAATCCAATCAAAGTACTCTATCGAAAAAGAATTTTCAGACAGAGCATCTAATTTGCTTGTAGCAAATGAAGATGGAAGATTTCTTGAGGAGATCCTCTTTGCTGAGTATTCGCTTGATGGTGGAGTTGTTAACAAAACTAATGTTGCAGATCTCCTTTCTGAGTCTAAAGACGAGCTCTCTATCAATAATGTAGAAGATAAGGAACTTATCGTAGTTGGACCATCAAAGCTAATGGGAGCAATTGAGCTCAATGCAATCAAGGATGGATTTAATCTTGCAGATTCTACTCTTAAGAATGGATTTGCTGGGACATTCTTGGGGCTTGAGTGTTACAAGAACAACCACTTGCCTCACACTACTAAGCTTAGCAAAGTACCAGCTGATGGAGATTCAATTAAAATCAATGGGGTTAAGATTACTTTCAAGACAACTCCTGTGGCTGCTGGAGATGTAAAAGTAGGAGCAAACTTACAAGCTTCTATTGAAAATGCAAACAAAGTACTTCTTGGAGCAACTGAGGATTCAGCAACAGGTAAAGCACTTGCTGATGAAGACAGAAAAAAGGTAAAGAGAGTAAGAGCAAAGATTATCCTTACTGGGGCAAGCGAAGCTCATGTCTTTACTTCTGGGAAGGCGAGAATTGAACTTACAGGGTTTGCTTCTGAAAAGACCTATGTATATGCAATGGTTGGACAGAAGAAGACTATTGATTGCGTAATTCAAGATATGCCAGAAATTCAGCAGAATAAGGCTGAGAAAAGAAAGGGATACTTTTATATGGCAACGGATCTCTTTGGGGTTAAAGCTTTCAAAGAGGGAAGAGAAAGAATGCTTAAACTTAAGATGGAAGCCTAA", "aa": "MAGTNDVQAFIPSIWSKRTQLMKREKSIIYAIANFEERAGLQFGAEVFRPFMMDDLEINQYTRGKDGKEQTLKTMPEKLLIDQENEITVYIDTNDQIQSKYSIEKEFSDRASNLLVANEDGRFLEEILFAEYSLDGGVVNKTNVADLLSESKDELSINNVEDKELIVVGPSKLMGAIELNAIKDGFNLADSTLKNGFAGTFLGLECYKNNHLPHTTKLSKVPADGDSIKINGVKITFKTTPVAAGDVKVGANLQASIENANKVLLGATEDSATGKALADEDRKKVKRVRAKIILTGASEAHVFTSGKARIELTGFASEKTYVYAMVGQKKTIDCVIQDMPEIQQNKAEKRKGYFYMATDLFGVKAFKEGRERMLKLKMEA", "aa_hexdigest": "a86558087f19dec12eb66f46b96317ac", "start_type": "ATG", "rbs_motif": "TTCTT", "ups": { "uniparc_id": "UPI000DBFD20B", "uniref100_id": "UniRef100_A0A328EKT8", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBFD20B", "UniRef:UniRef100_A0A328EKT8" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EKT8", "uniref90_id": "UniRef90_A0A839JT82" }, "psc": { "uniref90_id": "UniRef90_A0A839JT82", "product": "N4-gp56 family major capsid protein", "uniref50_id": "UniRef50_A0A839JT82" }, "pscc": { "uniref50_id": "UniRef50_A0A839JT82", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A839JT82" ], "product": "N4-gp56 family major capsid protein" }, "genes": [], "id": "NKOCPLAKPK_1417", "locus": "GCA003260355_01383" }, { "type": "cds", "sequence": "contig_1", "start": 750743, "stop": 750928, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACTATAAAAACCGAGTTTCTTGATCTGGTTAGAGAGGAAACAGAACTTAATCTCCAACAATGCTCAGATGCTCTACTCATGACGTATCTAAATCAAGTGAGAGATGATATTTTTAGTTTTGCTTCAACCTTCAACGGGAATCATTTTACGTGGAATTACTGGATCACTGACCTCATAGCATGA", "aa": "MTIKTEFLDLVREETELNLQQCSDALLMTYLNQVRDDIFSFASTFNGNHFTWNYWITDLIA", "aa_hexdigest": "45ad6409e7d88975f74c01e1c15d288c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7238.0594, "isoelectric_point": 4.161830711364746 }, "id": "NKOCPLAKPK_1418", "locus": "GCA003260355_01384" }, { "type": "cds", "sequence": "contig_1", "start": 750944, "stop": 751090, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAGAAAAGGCTCAAGATACAAAGTATGATATGAAAAAAATCCTGTCTGTTTACAGGAAGAATGAAAATAACGAATGGAATAAGCTAAAATCCTATGATTTGGAGGCTCTTCCTCATTCCCTATCTGAACTGGAGCAGAATTGA", "aa": "MAEKAQDTKYDMKKILSVYRKNENNEWNKLKSYDLEALPHSLSELEQN", "aa_hexdigest": "79d5d8c802640dce13e1e443b5c29e55", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5744.381699999999, "isoelectric_point": 5.699836921691895 }, "id": "NKOCPLAKPK_1419", "locus": "GCA003260355_01385" }, { "type": "cds", "sequence": "contig_1", "start": 751199, "stop": 751423, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAATGCTGAAGATACGATGGATGCACTCCTAATTCCTAGAGAGTATCAGACACTCCTCTGGGTTGGATTGACTGCAAAAATCTATCAATACAAAAAACAATATGACGAGAGAAATCTTGCTCTCCAAAACTACGAGAACGAAAAGCTGAAATTCAAAGCTACGAGTGGAGAAATGCAACAGATCTATTATGCAAATGCTAATGATTTATCTGAATTTGAATAA", "aa": "MNAEDTMDALLIPREYQTLLWVGLTAKIYQYKKQYDERNLALQNYENEKLKFKATSGEMQQIYYANANDLSEFE", "aa_hexdigest": "489454245ad0174af313d0b6f1a1649a", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8806.786299999996, "isoelectric_point": 4.72192211151123 }, "id": "NKOCPLAKPK_1420", "locus": "GCA003260355_01386" }, { "type": "cds", "sequence": "contig_1", "start": 752081, "stop": 752233, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAGTGGACTAAAGGGACAGTACAAGTTTTGATAGAATATACAGGGGAAGAAAAGACCAAAAATGGGAAAAACTACGACTCTGTAGAGACGACTAAGACTTATACCGAGACTCTTAGAGCTGAGAGGAATGGGCTCATAAATAAAATCTGA", "aa": "MEWTKGTVQVLIEYTGEEKTKNGKNYDSVETTKTYTETLRAERNGLINKI", "aa_hexdigest": "18d582f526082159d7d00b47fd47190f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5782.4051, "isoelectric_point": 6.188368034362793 }, "id": "NKOCPLAKPK_1421", "locus": "GCA003260355_01387" }, { "type": "cds", "sequence": "contig_1", "start": 752552, "stop": 752857, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGATTATCTCTCTTACGCAGTACGGAAATTATCTCATGATCTATGCGAATGGAAGAGAAGATGCCTATCAAATTGTCTCAGATGGTTCAGGTTCTGCCACTGAGAGTACTTTTATCTGGAAAGGAATGCAATTTGTCTCAGTATCAAATGATGGAGGACAAGACTTTGTTATTACAGAGAGGCAAAATAGTTACCAATTTTGGGCAGTAAGTGGGACAAATAAGCAGATACTTTTTAGCTCTGAGAGAATTTTTACTCAGAAAAATATGAGAGAGAAAGAAAAGTATCTGTTTGGCTTCTGA", "aa": "MKIISLTQYGNYLMIYANGREDAYQIVSDGSGSATESTFIWKGMQFVSVSNDGGQDFVITERQNSYQFWAVSGTNKQILFSSERIFTQKNMREKEKYLFGF", "aa_hexdigest": "8394d0494e8d253e6d88c68b1ca55d82", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11625.901299999992, "isoelectric_point": 6.193938255310059 }, "id": "NKOCPLAKPK_1422", "locus": "GCA003260355_01388" }, { "type": "cds", "sequence": "contig_1", "start": 752954, "stop": 753175, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTAGGTTTACCCTTGATCAAGGAGTAAAAATTTCACATATCCACGAAGAAAATCAGGACTCAATTCTCTGTTGCACCACTAATAGGGCAACAAAAATCAATAGTATGCAGAGGATCCAGCTCAATAATGATTACAATACATGCTTTGATGGAAGTATCACACTCCCTACTCTCGTAAGTCCAATAGAAGAAGGACAGCTAGAAAGCATCAGATTTTGA", "aa": "MSRFTLDQGVKISHIHEENQDSILCCTTNRATKINSMQRIQLNNDYNTCFDGSITLPTLVSPIEEGQLESIRF", "aa_hexdigest": "4ddf9a2a33182ec14c7c449c5ac8d85e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8317.2768, "isoelectric_point": 5.107915687561035 }, "id": "NKOCPLAKPK_1423", "locus": "GCA003260355_01389" }, { "type": "cds", "sequence": "contig_1", "start": 753182, "stop": 753439, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCTAACGAAAAGAGTAAAATAGAGCTTCGAGCGATGATTAACTCAAAAGACTACTTCACTTTTGAGGTAAAGAATGCTGAGGGGAAGAATTTTTCAAAATGCAAACTTCAAAATCAGCAAGGAGCATGGCACTTAGAACTGATAGATTCAAAACTTGATAATGGTGTCGGGTATGTAAGCTTTAGAACTGTTGGAGAGCTTGAGAAAGTAAAAACCTTTAATGCCGATACAACTCTCCTAGATATGAATGGATGA", "aa": "MPNEKSKIELRAMINSKDYFTFEVKNAEGKNFSKCKLQNQQGAWHLELIDSKLDNGVGYVSFRTVGELEKVKTFNADTTLLDMNG", "aa_hexdigest": "83644067bb2bfbfb45694e44794b3352", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9675.877799999993, "isoelectric_point": 6.564187431335449 }, "id": "NKOCPLAKPK_1424", "locus": "GCA003260355_01390" }, { "type": "cds", "sequence": "contig_1", "start": 753870, "stop": 754142, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCCGCACAACCAGGACTTAATATGATGCCACTCAATGCGAGATTGCAGGTAGGGAGTAAGTATTTGGATCAATGTAATATATCTGGGTATGTGGTGAAGAATTTTGATGGAAGCTGGGGTAATTTAACAAAAATTCCTCTCATCAATAAGATTAACTCACTAGATGAGATCAATCATGAATGGCATCAGGGGAATATGAAAGACTTTGCGACTAAGAAATTTTTTGAGAAAGAGGTAGTTGGAAAGATTGATTGGCAGGTTAAAGATTGA", "aa": "MPAQPGLNMMPLNARLQVGSKYLDQCNISGYVVKNFDGSWGNLTKIPLINKINSLDEINHEWHQGNMKDFATKKFFEKEVVGKIDWQVKD", "aa_hexdigest": "b41bdc93763c00048b7a2f3c57af1eb4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10323.755999999994, "isoelectric_point": 7.854457283020019 }, "id": "NKOCPLAKPK_1425", "locus": "GCA003260355_01391" }, { "type": "cds", "sequence": "contig_1", "start": 754817, "stop": 755035, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGAGAAAGTAGTTGAGAAGAAAATGACACAAGAAGAAGCTCAGGCATATGCAGAACTTAAAGAGAAATCTGCGGTTATTGATGGGCTTTTTGAAGCTATTAAAACTAAAGATAAAGCAACTCAAGAACAAATAAAAAGTGTTGTTGAAGCATTCAAAAAAAGTAGTGATCAGTATACTAAGAATATCGGATTTTACTTGGGGTATTTGATGAGATAA", "aa": "MEKVVEKKMTQEEAQAYAELKEKSAVIDGLFEAIKTKDKATQEQIKSVVEAFKKSSDQYTKNIGFYLGYLMR", "aa_hexdigest": "dc44fe09296bb34358d2c3de029a1cf9", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8284.447400000001, "isoelectric_point": 7.879535484313965 }, "id": "NKOCPLAKPK_1426", "locus": "GCA003260355_01392" }, { "type": "cds", "sequence": "contig_1", "start": 755035, "stop": 755181, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGAAGAAAAATCAGGATTCTTCTCTTTTTTCTTATATTCCTTGCTGGATGCTCAAATGGTAAAATTGAAGAAAAAAGAGATACAACGATTGTCGATTTATTAAACGATGGAAACAGTATTATCTCTTGTGATTTAAACATATGA", "aa": "MGRKIRILLFFLIFLAGCSNGKIEEKRDTTIVDLLNDGNSIISCDLNI", "aa_hexdigest": "e84550d3bdb0e425794bcf0f543351a3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5384.2982999999995, "isoelectric_point": 6.022341346740721 }, "id": "NKOCPLAKPK_1427", "locus": "GCA003260355_01393" }, { "type": "cds", "sequence": "contig_1", "start": 755218, "stop": 755394, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATACGCTATCATTTAATGGAAATCAGAAAGAATCAAAACCACTTCAAATTATCATTACAGATATAGCATCTGAAGAACCAAAACTTAAGGGGAATGCTGATGAAGTGGAGCTAATAAAACTTCCTACAGGGAATCCTGATACTGTTTATATGCTTGAAGAAACTGGTTTTTGA", "aa": "MDTLSFNGNQKESKPLQIIITDIASEEPKLKGNADEVELIKLPTGNPDTVYMLEETGF", "aa_hexdigest": "e42200e3a7efc5679442b72e642ae717", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6424.174799999999, "isoelectric_point": 4.25061321258545 }, "id": "NKOCPLAKPK_1428", "locus": "GCA003260355_01394" }, { "type": "cds", "sequence": "contig_1", "start": 756036, "stop": 756230, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTATTACAAGAGGACTATCTGTCAGAGCGTTTGTTGTCAATGGTATACTAAAAGTTCAGATAGGGAGGATGATTAAACAAAAAGAAGAATGGCTTGACATCATCCAAACAAGTTTAGCGAGTAAAGTTTTATTATATCCGATGACGGCGGAAGAGTTTAATGCAAACGATGACATTTTATTTGAGGATTGA", "aa": "MFITRGLSVRAFVVNGILKVQIGRMIKQKEEWLDIIQTSLASKVLLYPMTAEEFNANDDILFED", "aa_hexdigest": "f71e1bcaf1750cad84f88ba3f3cb7df4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7360.528599999999, "isoelectric_point": 4.9287023544311515 }, "id": "NKOCPLAKPK_1429", "locus": "GCA003260355_01395" }, { "type": "cds", "sequence": "contig_1", "start": 756330, "stop": 756503, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATAAGAAGTATTGGTAAGCAAAAGTCTATCGGTAAATATTTGGATTTTTTTAGGGCAGAAGCCCTAAAATTAGAAAAGAAATATAATATTGAGTATGAATATGCTCCATGCAATAATTATGCTTCAATATTTTTTGATTCCAGTAGAGATCAATCATTTACAAGGTTTTGA", "aa": "MIRSIGKQKSIGKYLDFFRAEALKLEKKYNIEYEYAPCNNYASIFFDSSRDQSFTRF", "aa_hexdigest": "922a15127e171fa5cf614af03bb388d4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6829.6819, "isoelectric_point": 9.166195487976076 }, "id": "NKOCPLAKPK_1430", "locus": "GCA003260355_01396" }, { "type": "cds", "sequence": "contig_1", "start": 757208, "stop": 757390, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAGGCGATGGATATACTCCAGAGTTTAAACTTGAAGGAACAGTCTTTAAGTGGAAGTTCCCAAATGAGAGTCAATGGACTACACTCTTTGATACAACTTCATTCAAGGGGGAAAAGGGAAATGATGGGGTATGAATCTCTGAGATTACACATACTAAGAGAGGTAAAACTACGACCATAA", "aa": "MEAMDILQSLNLKEQSLSGSSQMRVNGLHSLIQLHSRGKREMMGYESLRLHILREVKLRP", "aa_hexdigest": "5048f5fe2ea5597af1fd1aa1d56a1ccc", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6947.081999999997, "isoelectric_point": 9.976176261901855 }, "id": "NKOCPLAKPK_1431", "locus": "GCA003260355_01397" }, { "type": "cds", "sequence": "contig_1", "start": 757436, "stop": 757729, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAGAAAAAGGAAACGATGGTAAAAACCCAGAGTTCCAACTCTGATCGACCCACCTCCAGTGGAGACTCCAAGGAGAGAGTCAGCGAAAGAATCTCATTCCCAAAGAACAGATCAGAGGGGCTACTATTACTAATGTGGCAAAAACTGCGGGGAATGGGGCTCCATGAACTACCGATACTTATACGATCACTCTTTCAGACGATCAGACTTTCAGTTTCTCAGTGTATAACGGAAGAGATGGTAGCGGATCAGGAGATATGCTCAAAGCGAACAATCTCTCGGACGTGGTAG", "aa": "MEKKETMVKTQSSNSDRPTSSGDSKERVSERISFPKNRSEGLLLLMWQKLRGMGLHELPILIRSLFQTIRLSVSQCITEEMVADQEICSKRTISRTW", "aa_hexdigest": "70640bd4e10f61c730cd6f8a2f67c949", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11200.845999999989, "isoelectric_point": 9.4401216506958 }, "id": "NKOCPLAKPK_1432", "locus": "GCA003260355_01398" }, { "type": "cds", "sequence": "contig_1", "start": 757696, "stop": 757863, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTCAAAGCGAACAATCTCTCGGACGTGGTAGATAAAGCACAAGCAAGGGCTAATCTCGAGCTGTATTCAATAGCTCAAATAACTGCCCTTCTCCAGAATATGCTCACCTCTGAGAATCTTAATGCTGTTGCTAGAACATTAGCGGGGCTAACTGTAGTCCCATGA", "aa": "MLKANNLSDVVDKAQARANLELYSIAQITALLQNMLTSENLNAVARTLAGLTVVP", "aa_hexdigest": "009636e01cf207f599264c8c3207eea6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5882.763800000001, "isoelectric_point": 5.971413612365722 }, "id": "NKOCPLAKPK_1433", "locus": "GCA003260355_01399" }, { "type": "cds", "sequence": "contig_1", "start": 758152, "stop": 758649, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGATCCATCGGTAATTCCACTGATTGCAACCACCGAAACGGTAACGGTAGCGAATGAGGCAGCAAGGTTGAATTTAACGACAGCACAGGTCCAGAGAGGAGATTATGTCGTTCAAACCGATACTGGAGCGAAGTTTATTCTAAGCGGAAATAATCCCAAGAATAAAAATGATTGGATACCAATGTCGGATACCACTCCTGATCGGAGTCAAATCGCTAATAAACCTTCTTCATTCGCTCCTTCTCCACATTCACACACAAAAGCAAGTGTTGGATTAAGTAAGGTAGATAACACCAGTGATGCTGAAAAGAACTCAGCTACTGCAACCTTGACGAATAAGACGATCAATGGAGATAATAATACGATCACTAAAGTAAATAGTCTCAAAAACCAGAATGGAAATACGGAGATTAAGACTCGAGCAGGGACGAAGGCTCAGCATACTGCGGTAGGAACTAAGGATGCGAATACGCTTTACCTTGTAACCGAGAATTAA", "aa": "MDPSVIPLIATTETVTVANEAARLNLTTAQVQRGDYVVQTDTGAKFILSGNNPKNKNDWIPMSDTTPDRSQIANKPSSFAPSPHSHTKASVGLSKVDNTSDAEKNSATATLTNKTINGDNNTITKVNSLKNQNGNTEIKTRAGTKAQHTAVGTKDANTLYLVTEN", "aa_hexdigest": "5fa23834b1c29186eff6713cd79a99b7", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17567.181399999987, "isoelectric_point": 8.860808753967284 }, "id": "NKOCPLAKPK_1434", "locus": "GCA003260355_01400" }, { "type": "cds", "sequence": "contig_1", "start": 758649, "stop": 758750, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTACAACTAGGAGACAAAAAGATTTCTGAACTCTACCTCGGGGAAAAGAAAGTGTCTGAAGTTTATTTAGGGGAGGAGAAGATACGACCGGTTGGATGA", "aa": "MLQLGDKKISELYLGEKKVSEVYLGEEKIRPVG", "aa_hexdigest": "7067770b09fb16c1c4075fe6731aeccf", "start_type": "ATG", "rbs_motif": "TTGTA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3750.3627000000015, "isoelectric_point": 6.116807746887206 }, "id": "NKOCPLAKPK_1435", "locus": "GCA003260355_01401" }, { "type": "cds", "sequence": "contig_1", "start": 759445, "stop": 759642, "strand": "+", "frame": 1, "gene": null, "product": "Holin", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EKU8", "UniRef:UniRef90_A0A328EKU8" ], "nt": "ATGAACAACTTGAACTTTTTAGATGCATTGAAAGGAATCTTTGGCGGAGTCGCTCTTGCTGGGGTTTTGAATTATTTCGGGATGAGCGGGGAGATGCTCTCAATCTTATCAATCTTACTGTTAGTAGACTTTATCTTTGGAGTGCTTAGGGCAAAATACAAAAAAGAGTCTATAACTAGTGATAATATGAGTAGATGA", "aa": "MNNLNFLDALKGIFGGVALAGVLNYFGMSGEMLSILSILLLVDFIFGVLRAKYKKESITSDNMSR", "aa_hexdigest": "07c6018ebacfcfc568fb60808f2486a0", "start_type": "ATG", "rbs_motif": "TTATT", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 759445, "stop": 759933, "observations": { "mutation": [ 759640, 759877 ], "stop": [ 759640, 759877 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EKU8", "query_cov": 1.0, "subject_cov": 0.4012345679012346, "identity": 1.0, "score": 124.0, "evalue": 8.97e-35, "gene_start": 1, "gene_end": 65, "reference_start": 1, "reference_end": 65, "reference_sequence": "MNNLNFLDALKGIFGGVALAGVLNYFGMSGEMLSILSILLLVDFIFGVLRAKYKKESITSDNMSRGVIRKATRWCIPFIVVAIIRGAGIDGAERLSNTIMGILIVAEGYSILRHIYNINTNKDLPEIDVFDALMKKLTEFLISKGPKFPGEKSSKSDTERPE" }, "paralog": false, "identity": 0.9876543209876543, "score": 310.0, "evalue": 1.25e-110, "description": "Internal stop codon at 759640, 759877. Nonsense mutation around 759640, 759877.", "psc": { "uniref90_id": "UniRef90_A0A328EKU8", "product": "Holin", "uniref50_id": "UniRef50_A0A328EKU8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EKU8", "UniRef:UniRef50_A0A328EKU8" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EKU8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EKU8" ], "product": "Holin" } }, "id": "NKOCPLAKPK_1436", "locus": "GCA003260355_01402" }, { "type": "cds", "sequence": "contig_1", "start": 759643, "stop": 759879, "strand": "+", "frame": 1, "gene": null, "product": "Holin", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EKU8", "UniRef:UniRef90_A0A328EKU8" ], "nt": "GTGATTAGAAAGGCGACGAGATGGTGTATCCCTTTTATTGTTGTAGCAATTATCAGAGGAGCAGGTATTGATGGAGCTGAGAGACTTTCTAATACGATTATGGGGATTCTGATCGTTGCTGAGGGATATTCTATTCTCAGACATATTTATAACATAAACACAAATAAGGATTTGCCTGAAATTGATGTTTTTGATGCGTTAATGAAGAAATTGACTGAATTTTTGATCAGTAAATGA", "aa": "MIRKATRWCIPFIVVAIIRGAGIDGAERLSNTIMGILIVAEGYSILRHIYNINTNKDLPEIDVFDALMKKLTEFLISK", "aa_hexdigest": "aa27b7aab96d2262fe11a45bcb3e1159", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 759445, "stop": 759933, "observations": { "mutation": [ 759640, 759877 ], "stop": [ 759640, 759877 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EKU8", "query_cov": 1.0, "subject_cov": 0.48148148148148145, "identity": 0.987, "score": 154.0, "evalue": 2.16e-46, "gene_start": 1, "gene_end": 78, "reference_start": 67, "reference_end": 144, "reference_sequence": "MNNLNFLDALKGIFGGVALAGVLNYFGMSGEMLSILSILLLVDFIFGVLRAKYKKESITSDNMSRGVIRKATRWCIPFIVVAIIRGAGIDGAERLSNTIMGILIVAEGYSILRHIYNINTNKDLPEIDVFDALMKKLTEFLISKGPKFPGEKSSKSDTERPE" }, "paralog": false, "identity": 0.9876543209876543, "score": 310.0, "evalue": 2.03e-110, "description": "Internal stop codon at 759640, 759877. Nonsense mutation around 759640, 759877.", "psc": { "uniref90_id": "UniRef90_A0A328EKU8", "product": "Holin", "uniref50_id": "UniRef50_A0A328EKU8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EKU8", "UniRef:UniRef50_A0A328EKU8" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EKU8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EKU8" ], "product": "Holin" } }, "id": "NKOCPLAKPK_1437", "locus": "GCA003260355_01403" }, { "type": "cds", "sequence": "contig_1", "start": 760582, "stop": 760815, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGGATCTCGGCTACATTCAAGGCTACTACTTCTGTAATACTGCTGCAGACGTCAGACTAGCCCTCAGTAAAAAGCATATGTGCTACACTGGAGCAAGATATATCCGCTGGCATCAGACTGGAGTGAGTCATATGCTAACTCCAGACAAGGACGCAAATGCTGGACACTTGTTCGCCTTAACGGGGATTGATAACGAAAAATATCATCATCCAAACAGCTGGACTGAGTGA", "aa": "MKDLGYIQGYYFCNTAADVRLALSKKHMCYTGARYIRWHQTGVSHMLTPDKDANAGHLFALTGIDNEKYHHPNSWTE", "aa_hexdigest": "360ef8cd11bd778584012261c0f4e2b4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8860.903099999998, "isoelectric_point": 7.845818519592286 }, "id": "NKOCPLAKPK_1438", "locus": "GCA003260355_01404" }, { "type": "cds", "sequence": "contig_1", "start": 760912, "stop": 761079, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGTGATTGATTCAGCTGATAGCGAAAAAATGGTTCAGATGAATATCCGAAATGGTAAGTTGCCGAATGAATCATTGATCAAGCTCCACGCAGTATTGATGGTTATGAGAGCTTTCTATAATGAGTTTGATAATGACAGGGCAATTCAGAAAGCCCTACAGCTCTGA", "aa": "MVIDSADSEKMVQMNIRNGKLPNESLIKLHAVLMVMRAFYNEFDNDRAIQKALQL", "aa_hexdigest": "988cd3cfe9a219aeec16f231eab41f0c", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6351.381800000001, "isoelectric_point": 6.5344038009643555 }, "id": "NKOCPLAKPK_1439", "locus": "GCA003260355_01405" }, { "type": "cds", "sequence": "contig_1", "start": 761366, "stop": 761683, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATTTCGAAAAAGCAAAAAAAGCATATGATAGCATGAGTTGGCAACAACAGAAGCAATTCGCTGAACAGAATAAGAATAATGCTGAATTTCAGCAATTCGCTCAGCAATATCTCGGAGGGAACAAACAGACTCCTCAAGCAACTCAACCGAACAATCCTGCACCCGTAGAAGACAATCAACAAAATCAGGATACTCAAGACGGTCAGAACTGGGGAAACAATGGACGGATCCAGTCTTCTCAAAATCAAGAGACACAGGCTCAAGGAATACCACAGCAACCAGCTCCACAAGATTCGCAAAACTATCAATTCTGA", "aa": "MDFEKAKKAYDSMSWQQQKQFAEQNKNNAEFQQFAQQYLGGNKQTPQATQPNNPAPVEDNQQNQDTQDGQNWGNNGRIQSSQNQETQAQGIPQQPAPQDSQNYQF", "aa_hexdigest": "174b0c0fc7cf95fd6b8b94ce2e88de46", "start_type": "ATG", "rbs_motif": "TTCTT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12022.572999999997, "isoelectric_point": 4.504399299621583 }, "id": "NKOCPLAKPK_1440", "locus": "GCA003260355_01406" }, { "type": "cds", "sequence": "contig_1", "start": 761789, "stop": 762073, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAAATCTCAAGCCCCACAGGTATCGCAGGGGGAAATCTCTAATACCGTGAAGAATATCCAAAGGCAATGGCTTCAGCATTCAAGGATTGGGATGATGGGAACGGCTAGCGTTGATCAGATCAGAAATGGAATCCTGAATGGTCAATTTAGTCAGTCTGATTTAGAACTTTTAAGAACTCAAAATCCTCAGAAATATCAAGAATATCTCGCTCATGATGAGAAGTTGAATGCAATGTCTAACGCAAAGCACAATGCCAACCTCACGAATAAAATGTATAGGTGA", "aa": "MKSQAPQVSQGEISNTVKNIQRQWLQHSRIGMMGTASVDQIRNGILNGQFSQSDLELLRTQNPQKYQEYLAHDEKLNAMSNAKHNANLTNKMYR", "aa_hexdigest": "4ebdbcb59ecb575517a12e1247562a14", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10774.003499999992, "isoelectric_point": 9.694191551208494 }, "id": "NKOCPLAKPK_1441", "locus": "GCA003260355_01407" }, { "type": "cds", "sequence": "contig_1", "start": 762113, "stop": 762307, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAGGCTATGGAAAAGTTTTTTGGGAATATCAACTATAACAGCAAGGAAATTGTAGAATCCTATAAACAAGCTCTAAACTCCCCAGAAATCCAAAGACGGAACTCAAAAGTGCTAACCGCCCAAAGGGAGATGGATGAGATTGATGAAGATTTAGATACCATGAGGGAAGACCTCACAAGACAATATCCGTGA", "aa": "MEAMEKFFGNINYNSKEIVESYKQALNSPEIQRRNSKVLTAQREMDEIDEDLDTMREDLTRQYP", "aa_hexdigest": "b891b78bad15d0db240f5a11d082c75f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7660.4361, "isoelectric_point": 4.587213706970215 }, "id": "NKOCPLAKPK_1442", "locus": "GCA003260355_01408" }, { "type": "cds", "sequence": "contig_1", "start": 762359, "stop": 762463, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGAAGGAAAAATGAGCTCCATAGGGACTATATTATGGCAAGAGGAGAAACTCAATTTTTAATGAATTTGGCTGAGAAGAACTTTGAGGCTGACATGAAGTGA", "aa": "MRRKNELHRDYIMARGETQFLMNLAEKNFEADMK", "aa_hexdigest": "888534b6adf74ad0a29efd1ec12ad424", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4187.806200000001, "isoelectric_point": 8.211031913757324 }, "id": "NKOCPLAKPK_1443", "locus": "GCA003260355_01409" }, { "type": "cds", "sequence": "contig_1", "start": 762653, "stop": 762856, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGCACTTGAACCTCATTATAAAGAATTTGGCTCTATCATTCTCAGACCACAAGCTCAGGTAGCTCAAGACATCCTCGCTCTCGCAAAGAAAGAAGGAATCAGTGTAGGGGAAGCAATGAGGAAGAACTTTACCGAACCGTTATATAAAAAGCCTGAGTATAGGGCGATGATGAATAAAACATTGGGGATACAACCATGA", "aa": "MKALEPHYKEFGSIILRPQAQVAQDILALAKKEGISVGEAMRKNFTEPLYKKPEYRAMMNKTLGIQP", "aa_hexdigest": "e5e7f1bf9ad25c44ce03b82c7a5af1d3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7618.919199999998, "isoelectric_point": 9.572410774230956 }, "id": "NKOCPLAKPK_1444", "locus": "GCA003260355_01410" }, { "type": "cds", "sequence": "contig_1", "start": 763148, "stop": 763405, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF05257.22" ], "nt": "GTGAACAGTAGCACACCTTCTCTAGGGAGTGTGGCAGTTATTGACTGGACGAATAACCCTAATGTTAGTGAAGCTCAGAGAAAATATGGGCATGTTGGTGTGGTAATTGGAATCAATAAGGATGGAAGTGTTATTTTAAAGCAATCCAATAAAAATGGAGAAGAAAAAGTCTTTACTTCAACTTATAGAGCTGATCAGATTTATGGCTATTTTGATCCTACAATAAGTAAGGAACAAGCCACAAGCGGGAATGGCTGA", "aa": "MNSSTPSLGSVAVIDWTNNPNVSEAQRKYGHVGVVIGINKDGSVILKQSNKNGEEKVFTSTYRADQIYGYFDPTISKEQATSGNG", "aa_hexdigest": "d6b118c24ee1fa648aee654cac2febca", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF05257.22", "name": "CHAP", "length": 48, "aa_cov": 0.5647058823529412, "hmm_cov": 0.8125, "evalue": 1.0830588252504279e-08, "score": 31.966594696044922, "start": 3, "stop": 50 } ], "seq_stats": { "molecular_weight": 9167.973399999997, "isoelectric_point": 6.541281318664551 }, "id": "NKOCPLAKPK_1445", "locus": "GCA003260355_01411" }, { "type": "cds", "sequence": "contig_1", "start": 763508, "stop": 763675, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCGGATCCTAATGCTGATATTAATTCCATACTTGAGTATTCTGCAGGAGGGAAGGTATTGACAGACTCTTCAACAACTCCATTAATGAAATATCAAGATGCTCTTAATGGGATCGGAACACTTAGTGGACTCTTAAAACAAGCAAATACTGGTCCGATAGTATGA", "aa": "MSDPNADINSILEYSAGGKVLTDSSTTPLMKYQDALNGIGTLSGLLKQANTGPIV", "aa_hexdigest": "347d84d4c8c278c21d6a8673ed9e09e0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5682.346900000002, "isoelectric_point": 4.359459877014161 }, "id": "NKOCPLAKPK_1446", "locus": "GCA003260355_01412" }, { "type": "cds", "sequence": "contig_1", "start": 763781, "stop": 763951, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCTAACAGATGCAGACATTAAGCACTATTCTCAAACTGTTCCAAACTTACAATCTACAGAAGAGGTAAATAATGCTATTCTTGCTCTCACTTTAAATATGTTGGCACAAGGATACAAAAATAAACTGAGAACCCTAGCTTCTGCTGGTAATGATGTGTCTAGATTTTGA", "aa": "MLTDADIKHYSQTVPNLQSTEEVNNAILALTLNMLAQGYKNKLRTLASAGNDVSRF", "aa_hexdigest": "3ec126d20a2e839d09a9aa00113f7551", "start_type": "GTG", "rbs_motif": "TTTA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6180.931400000001, "isoelectric_point": 6.521671867370605 }, "id": "NKOCPLAKPK_1447", "locus": "GCA003260355_01413" }, { "type": "cds", "sequence": "contig_1", "start": 764207, "stop": 764353, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGTCGCAAGCTGAACAAGAAATCTATCGTTATCTTTTGCCTAAAGTAGTGGCAAAGAAACGAAATGAGGACAGGAAGCAGGCAAAAAATGAGTATTATAGCAAACTTATAAATTCAGACAATTCAGAAGAAAGACAGCTTTGA", "aa": "MKSQAEQEIYRYLLPKVVAKKRNEDRKQAKNEYYSKLINSDNSEERQL", "aa_hexdigest": "580e3b8ee7ae8dc38866bbde3d2c72b7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5833.5046999999995, "isoelectric_point": 9.345675468444824 }, "id": "NKOCPLAKPK_1448", "locus": "GCA003260355_01414" }, { "type": "cds", "sequence": "contig_1", "start": 764876, "stop": 765604, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTGAGAAAAGTCGCTAAAAGTGAGAATGGTCAAGCATTAATTCAAGCATATAATGAATTACCTAAAGAAGATAGAGAAGAGTTCAGTGATCTTTTAACCTATGCTAATGGATTGATGGATTTAGCTGGGATGAAAGCATTCAAACAGCCCGCCGAACAGGGTGTAAAAGCTCTTGCTACAGAAGGATTAAGCATACTCGGAGAAGTAGATAATTTAGCACAAGCAAGTGCAAAATCAGTAGCAAAAACCCTTGCCGAAAAATCAGCCCAAAAAAGTGTAGAATCCCTAGATAAAAATACGGCAAAAGCTTTAGAAACTGTAGGAAGAATCGTGCAAGGAGATGAAGAAGCAAAAAATATTGCCCTTGAGGTTTTTAAGAACCTAGACACTAAAGGGGTAAAAACTTATAAAGAACTTGCTGAAAGGTTGGGGGCAAGAGGAAAAGAACTCATGCAACAGGTTGATGATGAGCTCCTCAAAGATACAAAAGGCTACAATTTCTGGGATAATCTCGTAAAGAAAGAAGTTGAAACCGCAGGAGGAACAAAGAAGATAATAGAAACTCCGATCTCTGATGGGATTGAACAGCTCAAAGAACTTTATGGAAAAATAGGGGATCAAATCAGTTTTGCAAAAATGGAAGGGCTGGAAGATAAGTTTGCGAAAGAAGGGCTAAGTTTAAAAGAGATGAATGATCTTGCAAGGCAATACTCAAGCGAATTTTGA", "aa": "MVRKVAKSENGQALIQAYNELPKEDREEFSDLLTYANGLMDLAGMKAFKQPAEQGVKALATEGLSILGEVDNLAQASAKSVAKTLAEKSAQKSVESLDKNTAKALETVGRIVQGDEEAKNIALEVFKNLDTKGVKTYKELAERLGARGKELMQQVDDELLKDTKGYNFWDNLVKKEVETAGGTKKIIETPISDGIEQLKELYGKIGDQISFAKMEGLEDKFAKEGLSLKEMNDLARQYSSEF", "aa_hexdigest": "766ab5f4ed55c03d7402601bdb2ef2cf", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 26674.96940000001, "isoelectric_point": 5.050280952453612 }, "id": "NKOCPLAKPK_1449", "locus": "GCA003260355_01415" }, { "type": "cds", "sequence": "contig_1", "start": 765623, "stop": 765844, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGTGATCCAAAAACTTCAGTTTCCTCAGAACTCTATGAGTCACAAAGAAAGGCTATTAAAGAGTTTGTAAGAGAAAAAATGCCAGATGATGTAGTAAAGAACCTTGATAAACAATACAGTGAACTTTCTAATACTCGTGCTTGGGTTGAGAAAATGGCTGAGAAAGTTAATACTCTTGCACAAAAAACCAAGAAAAAAGGATTCCTAGAGAAAGCATGA", "aa": "MGDPKTSVSSELYESQRKAIKEFVREKMPDDVVKNLDKQYSELSNTRAWVEKMAEKVNTLAQKTKKKGFLEKA", "aa_hexdigest": "6b2377a710caa2e66316c26d3e89093a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8437.594699999998, "isoelectric_point": 9.397314643859861 }, "id": "NKOCPLAKPK_1450", "locus": "GCA003260355_01416" }, { "type": "cds", "sequence": "contig_1", "start": 765911, "stop": 766060, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCGAGTAATGTAGGGAATAAGATAAATAACAGTCTTGATATGGAAGCGGAATTGCCAAAATTACTTAAGGAAATGCAGGGATTGGTTGGAGAGATTAAAAAAGGAGAATCAACATTAAATTTAAAGCCTGGGGAAAGAGCTTCTTGA", "aa": "MPSNVGNKINNSLDMEAELPKLLKEMQGLVGEIKKGESTLNLKPGERAS", "aa_hexdigest": "9821c15c90b34e3ef96f157b22fc60d7", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5310.087799999999, "isoelectric_point": 6.155799293518065 }, "id": "NKOCPLAKPK_1451", "locus": "GCA003260355_01417" }, { "type": "cds", "sequence": "contig_1", "start": 766778, "stop": 766939, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EKT9", "UniRef:UniRef90_A0A328EKT9" ], "nt": "ATGAAATACCGTAAAGATTCAGAAAACGCTGTAAAATTTGATTATCCTTTAACAAAAGAGTCAAGAGGTGATATAGAACTCTTTGACCGTGAAGCGGGAGTATTTGTTGAGATAGCAAAGCTTACCAATGATCAATTAGCAGAAATTATTTCATATATTTGA", "aa": "MKYRKDSENAVKFDYPLTKESRGDIELFDREAGVFVEIAKLTNDQLAEIISYI", "aa_hexdigest": "5ae028b9c5b694acde1fa329ed5cdad9", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 766778, "stop": 767017, "observations": { "mutation": [ 766937, 767009 ], "stop": [ 766937, 767009 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EKT9", "query_cov": 1.0, "subject_cov": 0.6708860759493671, "identity": 1.0, "score": 104.0, "evalue": 2.84e-28, "gene_start": 1, "gene_end": 53, "reference_start": 1, "reference_end": 53, "reference_sequence": "MKYRKDSENAVKFDYPLTKESRGDIELFDREAGVFVEIAKLTNDQLAEIISYIGANGNEISEEEYKQLISEELKKESGE" }, "paralog": false, "identity": 0.9746835443037974, "score": 149.0, "evalue": 2.17e-48, "description": "Internal stop codon at 766937, 767009. Nonsense mutation around 766937, 767009.", "psc": { "uniref90_id": "UniRef90_A0A328EKT9", "uniref50_id": "UniRef50_A0A328EKT9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EKT9", "UniRef:UniRef50_A0A328EKT9" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EKT9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EKT9" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 6170.906799999999, "isoelectric_point": 4.72709445953369 }, "id": "NKOCPLAKPK_1452", "locus": "GCA003260355_01418" }, { "type": "cds", "sequence": "contig_1", "start": 767538, "stop": 767822, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATAATAAAGAAATTTGAGATATTGTTTCTAGGTATGTTGTATATGGTTGCTAGTATTTATCTTGTAGTAGTGCGTATGGTAAAACCTATAATAAAAACATATAAAGCGGTAGAACTTGCCATATTAGAAAAGAAGACGAAACCTACCATTTATAATAATGGTAGAAAATATATCCCAATACAAATACCCTCCAAGAGGTCTAAAAAAGGATACTCAATTAGATATATTAAAAAATCTGATTTAGATCATTATCTAAAAGAAAATGCTAAAGAGAACTATTGA", "aa": "MIIKKFEILFLGMLYMVASIYLVVVRMVKPIIKTYKAVELAILEKKTKPTIYNNGRKYIPIQIPSKRSKKGYSIRYIKKSDLDHYLKENAKENY", "aa_hexdigest": "b6d4650a49133767f3520b331e3d103d", "start_type": "ATG", "rbs_motif": "TTATT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11132.34909999999, "isoelectric_point": 9.96276683807373 }, "id": "NKOCPLAKPK_1453", "locus": "GCA003260355_01419" }, { "type": "cds", "sequence": "contig_1", "start": 768783, "stop": 768881, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTAATCCAACTGTAGCATTTCACCCAGGGGAATACATTGAAGAGGAACTAGAAGCTAGGAATTGGACTCAGGCTAAATTTGCTGGGATCATTTGA", "aa": "MFNPTVAFHPGEYIEEELEARNWTQAKFAGII", "aa_hexdigest": "6c95af9c4d47a8ae8bc8dbaf0f5cc551", "start_type": "ATG", "rbs_motif": "TTATT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3710.131299999999, "isoelectric_point": 4.636265754699706 }, "id": "NKOCPLAKPK_1454", "locus": "GCA003260355_01420" }, { "type": "cds", "sequence": "contig_1", "start": 769962, "stop": 770078, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAATCTAAAAATCTGATCCCTCATACTGAGATAGTCCACCACCCGGTAGGACTCACAGAATACAGACTCAGAACAGAGACCTTTAGACAGTTTCTCCAGGTCTTTGTTTGA", "aa": "MKKSKNLIPHTEIVHHPVGLTEYRLRTETFRQFLQVFV", "aa_hexdigest": "d0a3300cfd1940daf1f1c6e0989476ca", "start_type": "ATG", "rbs_motif": "TTATT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4594.345000000001, "isoelectric_point": 9.988038444519042 }, "id": "NKOCPLAKPK_1455", "locus": "GCA003260355_01421" }, { "type": "cds", "sequence": "contig_1", "start": 770315, "stop": 770446, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCTGCTCTTGGGTTTAAGTCTGAGGTTCTTGCTCTTAGTTTTGAACCTGAATACCGACTCTTAGAATCAGCACTGATTCCAGAGGAGGAGCTAGGGAAGTTTTTAATTGATAATATAAAAGTGGAATGA", "aa": "MSALGFKSEVLALSFEPEYRLLESALIPEEELGKFLIDNIKVE", "aa_hexdigest": "94472c828a834d02d72a309b9b57f6fd", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4869.581999999999, "isoelectric_point": 4.440739631652832 }, "id": "NKOCPLAKPK_1456", "locus": "GCA003260355_01422" }, { "type": "cds", "sequence": "contig_1", "start": 771644, "stop": 771769, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAAGAGAAGAAAAAATTACTGCCATCTATAAAGAGATGGCAAACAAGAAAAGAACATTTGGTTGTAGATGTAGAATAAATGATGGAAAAGATGTGATATTTATCAGGCAGAATAAAACCTGA", "aa": "MTREEKITAIYKEMANKKRTFGCRCRINDGKDVIFIRQNKT", "aa_hexdigest": "6f90518c985e9de391ae00839c98b28f", "start_type": "ATG", "rbs_motif": "TTATT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4907.723000000001, "isoelectric_point": 9.919121742248535 }, "id": "NKOCPLAKPK_1457", "locus": "GCA003260355_01423" }, { "type": "cds", "sequence": "contig_1", "start": 773390, "stop": 773776, "strand": "-", "frame": 3, "gene": null, "product": "Methyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1V5LEH5", "UniRef:UniRef90_A0A328EHW5" ], "nt": "GTGTTCCGCAAAAACTTTTCAGCCCAAGTGCTGAAAGCAAATATGAAAGTGGTCGGAAACTGTGAATACGGATTGATCCTTTACAGAGATAAATTGCCGAAGTTCAATAACCATGGAAAGATGATATTTAATTGTTTTGAGTGGGAGAAAGACACCCAAACCGCAAAAGTTCATCCAACCCAAAAGCCTGTCCAGCTTTTAGAGCAGTTGATAGAAATCTTCACAGATAAAGAGGAAGTAGTTATTGATCCGTGTGCAGGAAGCGGAACAACGCTTCTTGCAGCTGCGAGGTGCAATAGAAAATCCTTTGGATTTGAGATTAAAAAGAACTTCTACAACCTTGCAAAAGAAAAAATCCTTACCAATATTCAAAATAAGCTGTTATAA", "aa": "MFRKNFSAQVLKANMKVVGNCEYGLILYRDKLPKFNNHGKMIFNCFEWEKDTQTAKVHPTQKPVQLLEQLIEIFTDKEEVVIDPCAGSGTTLLAAARCNRKSFGFEIKKNFYNLAKEKILTNIQNKLL", "aa_hexdigest": "478d129ab22c895833ffbef594019afb", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI003080DF02", "query_cov": 0.828125, "subject_cov": 0.828125, "identity": 0.594, "score": 137.0, "evalue": 1.98e-39, "valid": false, "product": "site-specific DNA-methyltransferase", "uniref50_id": "UniRef50_A0A2M7FDF1" }, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 773390, "stop": 774076, "observations": { "mutation": [ 773800, 773986 ], "stop": [ 773800, 773986 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EHW5", "query_cov": 1.0, "subject_cov": 0.5059288537549407, "identity": 0.992, "score": 261.0, "evalue": 8.1e-87, "gene_start": 1, "gene_end": 128, "reference_start": 126, "reference_end": 253, "reference_sequence": "MKMLYEPKLYNDHFQNYKKYGIPKAQLIIADIPYNLGNNAYASNPAWYEGGDNANGESELAGKQFFDTDKNFKISEFLHYVNQMLKPEPKEAGKAPCIVVFCEYGQQFELIQKAPQYGFKNYINLVFRKNFSAQVLKANMKVVGNCEYGLILYRDKLPKFNNHGKMIFNCFEWEKDTQTAKVHPTQKPVQLLEQLIEIFTDKEEVVIDPCAGSGTTLLAAARCNRKSFGFEIKKNFYNLAKEKILTNIQNKLL" }, "paralog": false, "identity": 0.9912280701754386, "score": 464.0, "evalue": 3.2e-169, "description": "Internal stop codon at 773800, 773986. Nonsense mutation around 773800, 773986.", "psc": { "uniref90_id": "UniRef90_A0A328EHW5", "product": "Methyltransferase", "uniref50_id": "UniRef50_A0A1V5LEH5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EHW5", "UniRef:UniRef50_A0A1V5LEH5" ] }, "pscc": { "uniref50_id": "UniRef50_A0A1V5LEH5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1V5LEH5" ], "product": "Methyltransferase" } }, "id": "NKOCPLAKPK_1458", "locus": "GCA003260355_01424" }, { "type": "cds", "sequence": "contig_1", "start": 774229, "stop": 774408, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCTAGAGATAAAAGATAAATTCCTCGCAGATAATCCTTATTCAAAAGAGATTGAAGACTGTTTTACTCCTGCGATTAGTGATATTAACAACGACTACAATCAAAGAATCGGCAAAGAACAATATGTAAAACCTGAAATTAAGTGGGATTTTGATGTTATTACCGAGGAAGTAGATTGA", "aa": "MLEIKDKFLADNPYSKEIEDCFTPAISDINNDYNQRIGKEQYVKPEIKWDFDVITEEVD", "aa_hexdigest": "897caaa6fcf4186670645eb5149f267e", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7014.717100000002, "isoelectric_point": 4.255899238586426 }, "id": "NKOCPLAKPK_1459", "locus": "GCA003260355_01425" }, { "type": "cds", "sequence": "contig_1", "start": 774547, "stop": 774861, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A839JWC6", "UniRef:UniRef90_A0A839JWC6" ], "nt": "ATGAAACTACACTTTAGAAAATTAACGCAAGACACAAGCAAATTTACTTTAGAGTTTGTATCAGATGCGGTCAATACCATTTCTCCAGAACACTTTATAACTTTGAAGAGACCTAAACTAGATAAATCCAAAATTGAAAGTATTGTCCGTTCTAACTATGCAGAGCAAATCAGTAAAGGACTTTTCACTGAGGAAGCAGGAAAGAAAATGGAGGTAGAGATTAAGGAAGCTATAGAAAAAGAAAAGAGAAGGATTCTTAATCTTCCGGCTCCTACCATCTTTTCAGTCTATGTCATTGAGGTTAAAAATCTCTGA", "aa": "MKLHFRKLTQDTSKFTLEFVSDAVNTISPEHFITLKRPKLDKSKIESIVRSNYAEQISKGLFTEEAGKKMEVEIKEAIEKEKRRILNLPAPTIFSVYVIEVKNL", "aa_hexdigest": "40f454766895aeefffcdcd164a694910", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 774244, "stop": 774861, "observations": { "mutation": [ 774429, 774549 ], "stop": [ 774429, 774549 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A839JWC6", "query_cov": 1.0, "subject_cov": 0.4444444444444444, "identity": 0.9620000000000001, "score": 192.0, "evalue": 5.26e-60, "gene_start": 1, "gene_end": 104, "reference_start": 1, "reference_end": 104, "reference_sequence": "MKLYFRKLTQDKSKFTLEFVSDAVNTISPEHFITLKRPKLDKGKIESIVRSNYAEQISKGLFTEEAGKKMEIEIKEAIEKEKRRILNLPAPTIFSVYVIEVKNLGETTAFKVDITKETALYFVQNKEKIAEMVFSLENYPFYLEGSSKSWKVLEIKDKFLADNPYSKEIEDCFTPAISDINNDYNQRIGKEQYIKPEIKRDFDVITEEVDGIVKGEKANVKDIIAKMNASISDE" }, "paralog": false, "identity": 0.9609756097560975, "score": 388.0, "evalue": 1.26e-139, "description": "Internal stop codon at 774429, 774549. Nonsense mutation around 774429, 774549.", "psc": { "uniref90_id": "UniRef90_A0A839JWC6", "uniref50_id": "UniRef50_A0A839JWC6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A839JWC6", "UniRef:UniRef50_A0A839JWC6" ] }, "pscc": { "uniref50_id": "UniRef50_A0A839JWC6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A839JWC6" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 12052.92479999999, "isoelectric_point": 9.362050437927245 }, "id": "NKOCPLAKPK_1460", "locus": "GCA003260355_01426" }, { "type": "cds", "sequence": "contig_1", "start": 774968, "stop": 775111, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATTAGGCAAGAGTTCTATCAACAGGATTTGGATCTTTCCGTTTTAAAAACGAAGATTGAACTCCTCCAGAACAGAACAACCGTTATCGGTGAGTATGTTAATATCGTTAAAATGCACCTTAAAAAGGATTTTATTATGTAA", "aa": "MIRQEFYQQDLDLSVLKTKIELLQNRTTVIGEYVNIVKMHLKKDFIM", "aa_hexdigest": "012b0cccc29c2150df584ff7f6e69f10", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5684.6936, "isoelectric_point": 8.08609218597412 }, "id": "NKOCPLAKPK_1461", "locus": "GCA003260355_01427" }, { "type": "cds", "sequence": "contig_1", "start": 775145, "stop": 775369, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EKW5", "UniRef:UniRef90_A0A328EKW5" ], "nt": "ATGGAAACAACAAAAATTGATAGCCTCTTGCAGAGACACGAAGTGCTAAAATTAAAAGGACTCCAACTCGGAGAACAAGACCTTTTTGACCGAGTAGCTCTCTGGGAAGAGATGGCTGATAGTATTACTGAACTCAGAAGCCAATACCAAGAACAGAAACAGGCAAATGAAGTAGAGAAAGGGAAAAGAATGATTGAGCTGAAAGCCGAGCTCGATGAAAACTGA", "aa": "METTKIDSLLQRHEVLKLKGLQLGEQDLFDRVALWEEMADSITELRSQYQEQKQANEVEKGKRMIELKAELDEN", "aa_hexdigest": "512152302bbefb7d91b62937e57abc81", "start_type": "ATG", "rbs_motif": "TTTA", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 774968, "stop": 775369, "observations": { "mutation": [ 775147 ], "stop": [ 775147 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EKW5", "query_cov": 1.0, "subject_cov": 0.556390977443609, "identity": 1.0, "score": 139.0, "evalue": 4.58e-41, "gene_start": 1, "gene_end": 74, "reference_start": 1, "reference_end": 74, "reference_sequence": "METTKIDSLLQRHEVLKLKGLQLGEQDLFDRVALWEEMADSITELRSQYQEQKQANEVEKGKRMIELKAELDENGKKKYTESTAEAVIRQEFYQQDLDLSVLKTKIELLQNRTTVIGEYVNIVKMHLKKDFIM" }, "paralog": false, "identity": 0.9924812030075187, "score": 255.0, "evalue": 3.61e-89, "description": "Internal stop codon at 775147. Nonsense mutation around 775147.", "psc": { "uniref90_id": "UniRef90_A0A328EKW5", "uniref50_id": "UniRef50_A0A328EKW5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EKW5", "UniRef:UniRef50_A0A328EKW5" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EKW5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EKW5" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 8718.771499999999, "isoelectric_point": 4.72970905303955 }, "id": "NKOCPLAKPK_1462", "locus": "GCA003260355_01428" }, { "type": "cds", "sequence": "contig_1", "start": 775468, "stop": 775962, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBFB0FB", "UniRef:UniRef100_A0A328EHF5", "UniRef:UniRef50_A0A839JT54", "UniRef:UniRef90_A0A328EHF5" ], "nt": "ATGACACGATTACCAAACAACTATGAACTTCCAAAATCTGAAGGAGGGAAGTATACCAAACTCCAAGATGGAACAACAAAAATCAGAATCCTAACCTCTCCAATTATTGGTTGGGAATACTTTACTAACGAGAATAAACCAGTCAAAAGCAGAATTGCTTATAGTGCAATTCCAGCAGACTCCAAAGATGGAAGAAAGGCTAAGGAGTTTTGGGCTTTCGTAGTGTATAACTACGATGAAAAAAGGGTTCAGGTGATGAGCATTACCCAAAAATCTCTTAAGGAACAACTCCTTGCTTTATCCAGAGACCCTGACTTCGGAGACCCAAAGGAATATGATTTAAAAATTACAAGATCAGGACAAAAATTAGAAACAACCTACACCATTATGGCATTAGGTAAAGCCGAGTTTACGGACGCAAAAGCTTTAGAAGAAGCAAATGGGGTGAGATTAGAGGCTTTATACGACGGGGAAGACCCTTTTGCTCCCTTTTAA", "aa": "MTRLPNNYELPKSEGGKYTKLQDGTTKIRILTSPIIGWEYFTNENKPVKSRIAYSAIPADSKDGRKAKEFWAFVVYNYDEKRVQVMSITQKSLKEQLLALSRDPDFGDPKEYDLKITRSGQKLETTYTIMALGKAEFTDAKALEEANGVRLEALYDGEDPFAPF", "aa_hexdigest": "1f379be25e9bb320c27875d85f2a02ad", "start_type": "ATG", "rbs_motif": "TTTA", "ups": { "uniparc_id": "UPI000DBFB0FB", "uniref100_id": "UniRef100_A0A328EHF5", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBFB0FB", "UniRef:UniRef100_A0A328EHF5" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EHF5", "uniref90_id": "UniRef90_A0A328EHF5" }, "psc": { "uniref90_id": "UniRef90_A0A328EHF5", "uniref50_id": "UniRef50_A0A839JT54" }, "pscc": { "uniref50_id": "UniRef50_A0A839JT54", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A839JT54" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18672.94799999999, "isoelectric_point": 6.398103904724121 }, "id": "NKOCPLAKPK_1463", "locus": "GCA003260355_01429" }, { "type": "cds", "sequence": "contig_1", "start": 776128, "stop": 776280, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTATGATGAATACTGAAACTGATGAGGATACCACATATTCGGCTATGTTTACAATAATGAGCCCTATGAAATATATGCAGAAAGTCTTTAATTCCTATTACTTTACGAATGACACCACAAGAGCAGAGAAAAATAGAGCAAAAGTCTAA", "aa": "MVMMNTETDEDTTYSAMFTIMSPMKYMQKVFNSYYFTNDTTRAEKNRAKV", "aa_hexdigest": "3b124f20a45e194cf4c0f5fc94037ee6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5963.768500000002, "isoelectric_point": 6.060196113586425 }, "id": "NKOCPLAKPK_1464", "locus": "GCA003260355_01430" }, { "type": "cds", "sequence": "contig_1", "start": 776285, "stop": 776533, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328ELE6", "UniRef:UniRef90_A0A328ELE6" ], "nt": "ATGGAAGAGATAAGAAAGCAAAAGGAGAAAATCCGGGCGGAAGTTATGCAACTCCTAACCATGCAACAGGGGAAACTCATTGAAGAAGTAAACCAACTCAGAGCAGAGAATGAGGAACTGAGAAAAATAAAAAACAATTACTCAAATCTTGAAGATAGAATTAGACAGCTAGAAATCCAAAAAAACAATACTCAGAGACTCTTAGAGAAGCTTAAAGAAGATAAGATTATATCAAAATTTGAGGACTGA", "aa": "MEEIRKQKEKIRAEVMQLLTMQQGKLIEEVNQLRAENEELRKIKNNYSNLEDRIRQLEIQKNNTQRLLEKLKEDKIISKFED", "aa_hexdigest": "586fea50542c95b0f83af5235480f60b", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 776186, "stop": 776533, "observations": { "mutation": [ 776254, 776257, 776263, 776287 ], "stop": [ 776254, 776257, 776263, 776287 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328ELE6", "query_cov": 1.0, "subject_cov": 0.7130434782608696, "identity": 1.0, "score": 142.0, "evalue": 3.17e-42, "gene_start": 1, "gene_end": 82, "reference_start": 1, "reference_end": 82, "reference_sequence": "MEEIRKQKEKIRAEVMQLLTMQQGKLIEEVNQLRAENEELRKIKNNYSNLEDRIRQLEIQKNNTQRLLEKLKEDKIISKFEDGDYGYDEYGNGGGYHIFGYVYNNEPYEIYAESL" }, "paralog": false, "identity": 0.9652173913043478, "score": 217.0, "evalue": 1.98e-74, "description": "Internal stop codon at 776254, 776257, 776263, 776287. Nonsense mutation around 776254, 776257, 776263, 776287.", "psc": { "uniref90_id": "UniRef90_A0A328ELE6", "uniref50_id": "UniRef50_A0A328ELE6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328ELE6", "UniRef:UniRef50_A0A328ELE6" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328ELE6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328ELE6" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 10012.423799999991, "isoelectric_point": 7.948838996887207 }, "id": "NKOCPLAKPK_1465", "locus": "GCA003260355_01431" }, { "type": "cds", "sequence": "contig_1", "start": 776710, "stop": 776823, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGAATAATAAATCAACTTTCATTGAGGAACATAAAATCTCAGAGTCAGAGTGGAAAGATGTAAAGTACAAAATCATCGGAAGGAAGACCTTGAGACATCCTAGAACTTGA", "aa": "MKNNKSTFIEEHKISESEWKDVKYKIIGRKTLRHPRT", "aa_hexdigest": "cddc93364e87d84d91d358a46b962643", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4529.1877, "isoelectric_point": 9.993905067443851 }, "id": "NKOCPLAKPK_1466", "locus": "GCA003260355_01432" }, { "type": "cds", "sequence": "contig_1", "start": 776827, "stop": 776937, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGATTCTTTACCTTACCAAAGACAGAACTTCATGAGCAGTTTATCCAAATGTTTTTTGAGCCTAGGAGAAAGATTACAGCAGAAAAGGAGAAAAACAGAATCCTTTGA", "aa": "MRFFTLPKTELHEQFIQMFFEPRRKITAEKEKNRIL", "aa_hexdigest": "ca137c8ba7f4ba1a4b0a0620d37988ae", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4554.3442, "isoelectric_point": 9.983912467956543 }, "id": "NKOCPLAKPK_1467", "locus": "GCA003260355_01433" }, { "type": "cds", "sequence": "contig_1", "start": 776983, "stop": 777099, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTTATACTAGACTTATGCAAGACTTATCAAAGCTTAAACCTACAATTTCCGAGGAAGAACAAGCAGAATTGAGGAAGTTTATAGAGGATATGATTGCAGTTGGGTATGAATGA", "aa": "MIYTRLMQDLSKLKPTISEEEQAELRKFIEDMIAVGYE", "aa_hexdigest": "007ddaa7d5c45186a5ab76046900b024", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4520.205500000001, "isoelectric_point": 4.711065864562988 }, "id": "NKOCPLAKPK_1468", "locus": "GCA003260355_01434" }, { "type": "cds", "sequence": "contig_1", "start": 777692, "stop": 777802, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGGAAAGTTTTATTATCAGGAAGAAGTATCAGGAGCAAATAAAACTCTTGAATACTGAGCAAAAAGCACAATTGCTGGATGCTATCTTTCACTATCAGACCAATTGA", "aa": "MKESFIIRKKYQEQIKLLNTEQKAQLLDAIFHYQTN", "aa_hexdigest": "74699728a4cfd67f8f63e503a54004b1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4412.0741, "isoelectric_point": 9.224345970153806 }, "id": "NKOCPLAKPK_1469", "locus": "GCA003260355_01435" }, { "type": "tRNA", "sequence": "contig_1", "start": 778297, "stop": 778368, "strand": "-", "gene": "trnR", "product": "tRNA-Arg(gcg)", "amino_acid": "Arg", "anti_codon": "gcg", "score": 51.9, "nt": "GCTCCGGTCGTTCAGTGGATAGGACACAATCCTGCGAAGATTGTAACCCCGGTTCGACTCCGGGTCGGGGCA", "db_xrefs": [ "SO:0001036" ], "anti_codon_pos": [ 778333, 778335 ], "id": "NKOCPLAKPK_26", "locus": "GCA003260355_01436" }, { "type": "cds", "sequence": "contig_1", "start": 778471, "stop": 778869, "strand": "+", "frame": 1, "gene": null, "product": "DUF2268 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MNR6", "UniRef:UniRef90_A0A660MNR6" ], "nt": "ATGAATAACTATGATTTGTGCATTTACCACCTTTCTCACAGAAAAAATATGTTTATCTTGGAAATAAAGCTCGATCTCAAGAAAGACCTACAAAATTGGGTGGATGGATGCAATAAAATAAGTCATGGCAAAAATTGGAAACTTGGAGTTTCTCCTGAATATCAACGTATTGTAGAGCAACTCGTTGGGAGCGATTTTGAAGAGGCTGAGAAATTTATGTATCCAGTTCTTGAAGGTATCTATGAAGAGAAGAAAAGTCTGATAACAAACTATAAAAACATCATACAAGAAAAGATCAATGTCGATCTACAAGAAGCCTGTCTCGCTATGGAGGATATGACAGGTTTTCCGCTCTATAGAAAAGATTTTATTCTGAATTTAACGACCTTTCCTCGTTGA", "aa": "MNNYDLCIYHLSHRKNMFILEIKLDLKKDLQNWVDGCNKISHGKNWKLGVSPEYQRIVEQLVGSDFEEAEKFMYPVLEGIYEEKKSLITNYKNIIQEKINVDLQEACLAMEDMTGFPLYRKDFILNLTTFPR", "aa_hexdigest": "29ecd494694598e545b463228ec41be1", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 778519, "stop": 779172, "observations": { "mutation": [ 778867, 778933, 779095 ], "stop": [ 778867, 778933, 779095 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A660MNR6", "query_cov": 0.8787878787878788, "subject_cov": 0.5132743362831859, "identity": 0.9309999999999999, "score": 225.0, "evalue": 7.8e-73, "gene_start": 17, "gene_end": 132, "reference_start": 1, "reference_end": 116, "reference_sequence": "MFSMKIQLDLKKDLQNWVDGCNKISHGKNWKLGVSPEYQYIVEQLVGSDFEEAEKFMYPVLEGIYEEKKGLITNYKNIIQEKINTHLQEACLAMEDMTGFPLYRKDFILNLTTFPRGPYNKETGEFWSCVFRSPENILGNFLHELQHFQVLHYFKDTPPMSTLTSEQFELLKESLTVILNVECKKFLAREDKGYPLHQDLRKNLLVFWAKERNFKALIAYGCDCVK" }, "paralog": false, "identity": 0.9354838709677419, "score": 417.0, "evalue": 5.26e-151, "description": "Internal stop codon at 778867, 778933, 779095. Nonsense mutation around 778867, 778933, 779095.", "psc": { "uniref90_id": "UniRef90_A0A660MNR6", "product": "DUF2268 domain-containing protein", "uniref50_id": "UniRef50_A0A660MNR6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A660MNR6", "UniRef:UniRef50_A0A660MNR6" ] }, "pscc": { "uniref50_id": "UniRef50_A0A660MNR6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MNR6" ], "product": "DUF2268 domain-containing protein" } }, "id": "NKOCPLAKPK_1470", "locus": "GCA003260355_01437" }, { "type": "cds", "sequence": "contig_1", "start": 778996, "stop": 779097, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTAGGCTTACCCGGGAACAATTTGAATTTCTCAAAGAGTCGCTTACGGTAATTCTCAATGTAGAATGTAAAAAGTTTTTAGCACGGGAAGACAAATGA", "aa": "MSRLTREQFEFLKESLTVILNVECKKFLAREDK", "aa_hexdigest": "3d60a5ae42140f9029fb2ff22e89fd29", "start_type": "ATG", "rbs_motif": "TTTA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4002.6590000000006, "isoelectric_point": 7.918216514587403 }, "id": "NKOCPLAKPK_1471", "locus": "GCA003260355_01438" }, { "type": "cds", "sequence": "contig_1", "start": 779209, "stop": 779469, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGATGTCGCGAAACTTGCTGGGATTCCAAAGCCGATTTTAGAGGAAGCGAAAGGCTTTTTATCTGCTTTAGAAAAAGGAAAAAAATCTGATCTTCCTTCTTTGAATGCTCCTCAGGGACTTTTCGCAGTTCAGCAAGAAGAGTTTGCTCATAAAGCCCAGTATGAAAAGGTAAAGGCGTTATTGGCAGGGATGGATTTGAATGCGATTACTCCGCTTCAGGCTTTGCAGCTTTTGATGAAGATTAAGGAGGAGTTGTAG", "aa": "MDVAKLAGIPKPILEEAKGFLSALEKGKKSDLPSLNAPQGLFAVQQEEFAHKAQYEKVKALLAGMDLNAITPLQALQLLMKIKEEL", "aa_hexdigest": "7e0933cf8ef56f93a2b4f5fb42c1d138", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9371.977599999997, "isoelectric_point": 6.569359779357909 }, "id": "NKOCPLAKPK_1472", "locus": "GCA003260355_01439" }, { "type": "cds", "sequence": "contig_1", "start": 779491, "stop": 779817, "strand": "-", "frame": 1, "gene": "mutS", "product": "DNA mismatch repair proteins mutS family domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A191HVF0" ], "nt": "GTGGGAAGTGGCGATATTATCGCGAAAAATCAGTCTACCTTTATGACGGAGATGATTGAGGTGGCGAATATCCTCAATAATGCGACCGAGAGGAGTTTCGTGATCTTTGATGAACTGGGGCGTGGAACCTCAACTTATGATGGACTAGCTCTTACCAAAGCAATTCTGCATTATCTTTTGAAAAACATTCAGGCAAAAACCCTGATCGCAACTCACTATCATGAACTCATCGCCCTTGAACAGGAAAGCGAGCAAATCAAAAATTTCTCAGTTAGTGTCTACGAAACCGATAAGGAAGTCGTTTTTATGAAAAAAATCAGTGCTTGA", "aa": "MGSGDIIAKNQSTFMTEMIEVANILNNATERSFVIFDELGRGTSTYDGLALTKAILHYLLKNIQAKTLIATHYHELIALEQESEQIKNFSVSVYETDKEVVFMKKISA", "aa_hexdigest": "9b31fcb8fb03e2de5e5a9e74911821a7", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A191HVF0", "query_cov": 0.8148148148148148, "subject_cov": 1.0, "identity": 0.58, "score": 97.8, "evalue": 1.3e-24, "valid": false, "product": "DNA mismatch repair proteins mutS family domain-containing protein", "uniref50_id": "UniRef50_A0A191HVF0" }, "pscc": { "uniref50_id": "UniRef50_A0A191HVF0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A191HVF0" ], "product": "DNA mismatch repair proteins mutS family domain-containing protein" }, "genes": [ "mutS" ], "id": "NKOCPLAKPK_1473", "locus": "GCA003260355_01440" }, { "type": "cds", "sequence": "contig_1", "start": 779944, "stop": 780456, "strand": "-", "frame": 1, "gene": "mutS", "product": "DNA mismatch repair protein MutS", "db_xrefs": [ "GO:0005524", "GO:0006298", "GO:0030983", "SO:0001217", "UniParc:UPI000CBF34F4", "UniRef:UniRef100_A0A2N8NGC9", "UniRef:UniRef50_A0A839JUW5", "UniRef:UniRef90_A0A2N8NGC9" ], "nt": "ATGAATCAAGGCTATTTCCTAGAGCTGACCAGTAAAGATAGCGAGCTTTTTGAGGAATTTCTCGCACAGCAAGATTTTACCGAATTGAGTGCTCAGGAGCAGGAAAAGTTTGCGATTGTCCGCAGACAAACCCTCAAAGGCAACCAAAGATATACGAGTCCTTATCTTGATAATCTCCAATCTCATATCCTCGGTGCCAAAGAGCAACTCAAGGTCAAAGAATCCGCAGTCTTACAAGGGCTTCAGCAGTCGCTTTTGGAAAGCATCACTCCGCTTTATAATTTAGCAGAAAAGCTGGCATGGCTGGATCTTTTTACTTCACAGGCGATTTTTGCTAGGGAATACCGCCTAGTCAAGCCACAACTCGCAGAAAACGGGATCATTGAGATTCAAGCAGGTCGCCATCTGGTGATTGAAGCCTTTTTGCCGAAGGATCAGCCTTTTATCCCTAATGCCTTGGAGATTGGCGAGTCCAAAAGCACGCATCACGGCTTGATTCACATCATCACTTGA", "aa": "MNQGYFLELTSKDSELFEEFLAQQDFTELSAQEQEKFAIVRRQTLKGNQRYTSPYLDNLQSHILGAKEQLKVKESAVLQGLQQSLLESITPLYNLAEKLAWLDLFTSQAIFAREYRLVKPQLAENGIIEIQAGRHLVIEAFLPKDQPFIPNALEIGESKSTHHGLIHIIT", "aa_hexdigest": "b019768df26e03318c97fb448ff41b36", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000CBF34F4", "uniref100_id": "UniRef100_A0A2N8NGC9", "db_xrefs": [ "SO:0001217", "UniParc:UPI000CBF34F4", "UniRef:UniRef100_A0A2N8NGC9" ] }, "ips": { "uniref100_id": "UniRef100_A0A2N8NGC9", "uniref90_id": "UniRef90_A0A2N8NGC9" }, "psc": { "uniref90_id": "UniRef90_A0A2N8NGC9", "gene": "mutS", "product": "DNA mismatch repair protein MutS", "uniref50_id": "UniRef50_A0A839JUW5", "go_ids": [ "GO:0005524", "GO:0006298", "GO:0030983" ] }, "pscc": { "uniref50_id": "UniRef50_A0A839JUW5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A839JUW5" ], "product": "DNA mismatch repair protein MutS" }, "genes": [ "mutS" ], "id": "NKOCPLAKPK_1474", "locus": "GCA003260355_01441" }, { "type": "cds", "sequence": "contig_1", "start": 780484, "stop": 781377, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF05192.23" ], "nt": "GTGCAGAAATTTATCCTTCAGCTTCGTCCGACCGAGATTATTATTGACACGGATACGAGGATCAAGGAAGAGCTGATTACGCCGATTCAGCAGTATTTGCAGTGTCTGATTTCCGTCTATGCAGTGCCGGTGGATCCCGGGCATTTCATCACTTCGCTCTGTAAGGTGCAACATACCAGCAGTTTCGGGAAAGCTTTGGAGAATGGGAGATTGGAGGCGATGGCACTCCTCTTTGCCTATCTTCAGCATACCCAGCAGCAACAGCTCACCAATATTTCAAAAATCAGATTTCATAGTTCGGAAGGCTTAGTTTTGATGGATGAAGTGACAATTAAGAACTTGGAACTCTTCGCTTCTAGTTATGAAAGTAGCACGAAATACAGCCTTTTCGGCATCTTAGATACGACCAAAACCGCAGGTGGCTCCAGACTTCTTCGCTATTTACTTGCAAATCCGATCAATATCAAGTCTGAACTTGACCGAAGACTCTCAAAACTTGAGGCTTTTTTGGAAGATCCGATGACCGCTGGGATTCATCAGTTTTTGGGGCAGTTTTTTGATATTCCAAAACTTTTGAGCTTGATTCTGTATAGAAAGCTCAGCCGAGGTCCGTTTATGAAGCTGAGAGCCGTGCTTCGTAATGCTTTACAAGGGCTAGAAGGGACGCTTCTGTCTGAACTTAAAAAAATCCAACTCACAGAGGAGGAAATCTCGGCACTTGATCAGATTTTTTTGCTTTTACAAAAGGCATTAAAGGCTGATGAAGAGCTTTTGGGAGAGTATGATTATGTCGCAGAGGGCTACGATGCGGAGATTGATGAGCTGAGGAAGATCGCTTATCATAGCGATGAAATTTTGATGCAATATCAACAGGAATTAGTCTCAAAAACCTGA", "aa": "MQKFILQLRPTEIIIDTDTRIKEELITPIQQYLQCLISVYAVPVDPGHFITSLCKVQHTSSFGKALENGRLEAMALLFAYLQHTQQQQLTNISKIRFHSSEGLVLMDEVTIKNLELFASSYESSTKYSLFGILDTTKTAGGSRLLRYLLANPINIKSELDRRLSKLEAFLEDPMTAGIHQFLGQFFDIPKLLSLILYRKLSRGPFMKLRAVLRNALQGLEGTLLSELKKIQLTEEEISALDQIFLLLQKALKADEELLGEYDYVAEGYDAEIDELRKIAYHSDEILMQYQQELVSKT", "aa_hexdigest": "464c3ea436816894cf7d7e421999fe8d", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF05192.23", "name": "MutS_III", "length": 154, "aa_cov": 0.5084175084175084, "hmm_cov": 0.948051948051948, "evalue": 3.881006731707647e-21, "score": 72.71604919433594, "start": 110, "stop": 260 } ], "seq_stats": { "molecular_weight": 33925.884700000046, "isoelectric_point": 5.501468849182127 }, "id": "NKOCPLAKPK_1475", "locus": "GCA003260355_01442" }, { "type": "cds", "sequence": "contig_1", "start": 781517, "stop": 781612, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTCGTTCAAGGAGTGATGATGGATTTGATGCTTCTCTCTACGATTTTTCAAGGGATAGGATCAGTGGTTTGCTCGGCGATTGCGACTTTATAG", "aa": "MVVQGVMMDLMLLSTIFQGIGSVVCSAIATL", "aa_hexdigest": "3e1682de4f8923d08a036619d43e8fb2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3228.9693999999995, "isoelectric_point": 4.0500284194946286 }, "id": "NKOCPLAKPK_1476", "locus": "GCA003260355_01443" }, { "type": "cds", "sequence": "contig_1", "start": 781663, "stop": 781860, "strand": "-", "frame": 1, "gene": null, "product": "DNA mismatch repair protein MutS-like N-terminal domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A191HVF1" ], "nt": "ATGAATCTTGAAAACCTCACTCCTGCGATGAAGCAATATGTGCAGATGAAGCAGCAAAATCCTGATTGCATTCTGTTTTTTCGTATTGGGGATTTTTACGAGGTCTTCTTTGAGGATGCGAAAATCTGTGCTTCAATCCTCGATCTCATCATTACTTCCAAAAATAAAAATTCTGAAAATCCGATTCCGATGGCGTGA", "aa": "MNLENLTPAMKQYVQMKQQNPDCILFFRIGDFYEVFFEDAKICASILDLIITSKNKNSENPIPMA", "aa_hexdigest": "8a640ce9c438559da2b02f2cfb606fc5", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A944DBW7", "query_cov": 0.9538461538461539, "subject_cov": 0.8157894736842105, "identity": 0.5, "score": 80.1, "evalue": 1.76e-18, "valid": false, "product": "DNA mismatch repair protein MutS-like N-terminal domain-containing protein", "uniref50_id": "UniRef50_A0A191HVF1" }, "pscc": { "uniref50_id": "UniRef50_A0A191HVF1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A191HVF1" ], "product": "DNA mismatch repair protein MutS-like N-terminal domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_1477", "locus": "GCA003260355_01444" }, { "type": "cds", "sequence": "contig_1", "start": 782048, "stop": 782140, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAAGCTCGCATTAACAAAAGATGAACTAGCAGATAGTGATAAAATTGAGATCATTCTAACAAAAGCCAAGGAGTTTATTGAAAAGTATTGA", "aa": "MKLALTKDELADSDKIEIILTKAKEFIEKY", "aa_hexdigest": "e77beae2ba69fbde5e4193741f60c161", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3498.089900000001, "isoelectric_point": 5.096036338806152 }, "id": "NKOCPLAKPK_1478", "locus": "GCA003260355_01445" }, { "type": "cds", "sequence": "contig_1", "start": 782210, "stop": 782776, "strand": "-", "frame": 3, "gene": null, "product": "Xylose isomerase-like TIM barrel domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_X1ACJ4" ], "nt": "ATGATCGGCTACGGCTTGGATATGATTTTTGATATGGCAAAAAGATGCGGGTATGACGGTATAGATCTCGCTATTTCAAAAGGATTTGATGCTCGAAATGAGAATTACGTAAAAAATCTCGTCAAAAAATATGAGCTTCCTATCTATGCAATTCAAACCTCTTCTCTTCTCAATAGCAAGGAAATGAATAAAGTCCTTGATCTTTGCGAAGCAACGGATTGCGATTTAATTACGATCAATGCCCCAAAGTTTTTTGATTTTAAGTCCTATTCCTTCATCTCAAATAATATTTCGGAATATCAAAATCAGAATCCCTCACTCTATTTTGCAATTATCAATCCAGAAGATACGAATGTATTTGCACTACCAATTCCTGCGTATCGTTTCAATAATGTCGTAGATATTATCAAAAAATACAGTTGTAATCTTGCTTTAGATATTGCAAATATGAATTCAGATGATCTAGAAAATATTTTTGTCAATAAATTAGATGATTTTGCGCCTTATTTGGCTTTGATTTATATTTCTGACAAATCAAAGTCTGGAGCGACACATTTGCTTCCTTGA", "aa": "MIGYGLDMIFDMAKRCGYDGIDLAISKGFDARNENYVKNLVKKYELPIYAIQTSSLLNSKEMNKVLDLCEATDCDLITINAPKFFDFKSYSFISNNISEYQNQNPSLYFAIINPEDTNVFALPIPAYRFNNVVDIIKKYSCNLALDIANMNSDDLENIFVNKLDDFAPYLALIYISDKSKSGATHLLP", "aa_hexdigest": "0c2d150b0fc777254e1f158c5ea8437f", "start_type": "ATG", "rbs_motif": "TTATT", "psc": { "uniref90_id": "UniRef90_A0A3D5YG98", "query_cov": 0.8085106382978723, "subject_cov": 0.987012987012987, "identity": 0.599, "score": 193.0, "evalue": 2.78e-60, "valid": false, "product": "Xylose isomerase-like TIM barrel domain-containing protein", "uniref50_id": "UniRef50_X1ACJ4" }, "pscc": { "uniref50_id": "UniRef50_X1ACJ4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_X1ACJ4" ], "product": "Xylose isomerase-like TIM barrel domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_1479", "locus": "GCA003260355_01446" }, { "type": "cds", "sequence": "contig_1", "start": 783138, "stop": 783503, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATGTTCTATGGTGTAATCGGAATCATTATTATGTTTAGTGCGAAATATCTGACTACGGTAATTTTTTCTGATATTTTCCTTGGAGGAGTAGGGGAGGCGATGACGACGACTGATTGGATTAATAAGCTCTATAATAACATCGTTTTCCCATTCATAAAAATCGCTATCTATCTTTCACTAGGATTTTTGGTAATTATGATGATGATCCGCGTCTTTACCTATATTACTGCACAAGATGACGGAACCAAAAAGAAAGCACTTGGAGTGATCACTTGGACAACGATTGGAATGCTCTTTATAATTGCAGCCAAGCAAATTGTAGAAGCAGTGTATGGAAAACAGGAAACAGTTCTAAAAACCTGA", "aa": "MMFYGVIGIIIMFSAKYLTTVIFSDIFLGGVGEAMTTTDWINKLYNNIVFPFIKIAIYLSLGFLVIMMMIRVFTYITAQDDGTKKKALGVITWTTIGMLFIIAAKQIVEAVYGKQETVLKT", "aa_hexdigest": "a6e565d6560dbc12d7c6a10b56e8911c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13599.286699999988, "isoelectric_point": 9.188308143615721 }, "id": "NKOCPLAKPK_1480", "locus": "GCA003260355_01447" }, { "type": "cds", "sequence": "contig_1", "start": 783510, "stop": 783650, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATCCAAAGCTGCTGCAATTATCAAAAAGGTCGTTGATGTGCTTTTCCCAGTAATTTTGATTGCCGGCGTCCTAATAGCGATGATCGGGCTTTACCAAGTCTTAGTTTCTGGTGATGCAGCAAAAGTAAAGGAGTGA", "aa": "MKSKAAAIIKKVVDVLFPVILIAGVLIAMIGLYQVLVSGDAAKVKE", "aa_hexdigest": "3041b9cbdd6956496406f4c204627a89", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4824.956700000002, "isoelectric_point": 9.602839851379393 }, "id": "NKOCPLAKPK_1481", "locus": "GCA003260355_01448" }, { "type": "cds", "sequence": "contig_1", "start": 784225, "stop": 784497, "strand": "-", "frame": 1, "gene": null, "product": "DUF1385 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MZY8", "UniRef:UniRef90_A0A660MZY8" ], "nt": "ATGATAGCGATTGTTTCAGGCTTCTCTTTCTCTCTAACCCGTATCCAGCAAGATGCTACACAAAAAAGAAAAATGCTGATTTCCTTCCTTCCATTTTACAGTGAATACCAGCGGTTTTCAGCCAAACACTTTGAGAAACCCTATCGATGGAGCAAAGAGGCTCAAGTATGGCGATTTATGATAGCTCTTTTTACGCTTTTCTCTCCTTCATTTCTCGTGATGCTATGCACTGTATTCTTGCTTTTTATGAGGGTTATTTTACTGCTCGGATGA", "aa": "MIAIVSGFSFSLTRIQQDATQKRKMLISFLPFYSEYQRFSAKHFEKPYRWSKEAQVWRFMIALFTLFSPSFLVMLCTVFLLFMRVILLLG", "aa_hexdigest": "0728ecd0cc2251c4bef7da4e039e46b5", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 783922, "stop": 784497, "observations": { "mutation": [ 784107, 784227 ], "stop": [ 784107, 784227 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A660MZY8", "query_cov": 1.0, "subject_cov": 0.4225352112676056, "identity": 0.9329999999999999, "score": 158.0, "evalue": 2.48e-47, "gene_start": 1, "gene_end": 90, "reference_start": 1, "reference_end": 90, "reference_sequence": "MIAIVSGFSFSLTRIQQDTTQKRKMLISFLPFYSEYERFSAKQFEKPYRWSKEAQVWRFMIALFTLFSPSLLVILCPVFLLFMRVILLLGGYDILPQEQKIRLHQLFKRYPEEIPISLLILSKKDANQEAGNLKEELEFYQDTYIAPKSTKDKIFYSLLTLSVLGLLGRWIWTSNLRWKGLFLIRRLARMLTMNLTSTLIPIFPLSYWIKKKI" }, "paralog": false, "identity": 0.93717277486911, "score": 357.0, "evalue": 3.68e-128, "description": "Internal stop codon at 784107, 784227. Nonsense mutation around 784107, 784227.", "psc": { "uniref90_id": "UniRef90_A0A660MZY8", "product": "DUF1385 domain-containing protein", "uniref50_id": "UniRef50_A0A660MZY8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A660MZY8", "UniRef:UniRef50_A0A660MZY8" ] }, "pscc": { "uniref50_id": "UniRef50_A0A660MZY8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MZY8" ], "product": "DUF1385 domain-containing protein" } }, "id": "NKOCPLAKPK_1482", "locus": "GCA003260355_01449" }, { "type": "cds", "sequence": "contig_1", "start": 784564, "stop": 784782, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATAGGAAACTCCATTATTCTCCACCTGTAACGTTTTCGGCCCTTTCTGGTTTGCTATTTTTCTCTTTTTTTGTTCAGCACTCAATAAAAAAGAAGCAACAACAGTGGAGACCTGATGAGTATTCCTTCGTTGCCTCTCGATGTAAATTTGGAAATCGGCTATGGTTGCTCTTGCTTTTGTTTGTTGGAAGCTTCGCAATTGGAAGCGGGACTTGA", "aa": "MDRKLHYSPPVTFSALSGLLFFSFFVQHSIKKKQQQWRPDEYSFVASRCKFGNRLWLLLLLFVGSFAIGSGT", "aa_hexdigest": "b14287c1a0c5048c21e9b5fd9f3eb7e6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8331.630199999996, "isoelectric_point": 10.129224205017088 }, "id": "NKOCPLAKPK_1483", "locus": "GCA003260355_01450" }, { "type": "cds", "sequence": "contig_1", "start": 784934, "stop": 785512, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATCTATCATGGTCCTAAAATTTATCCTCATACCACTTGTAAACAGGAAACAAGGGAAATTTGTCAGATGACGCGAGAAGAGCTCCAAAAATGTAAACTTAAAAATGGAGAAGATATTCTTTTATTAGAAGAATTCCTTCCACAGGTAAAGAATCGGTTTTCTTTCTTTTTCCTAGACTTCAAAACTTCTACCAATCCTCAATGCAAACAAAATAATCAGCTCCTTCTCGACAAGGCTATTGCATTGGTGAAAAAATATAAGATGGATGGAAATACGATTTTTTCTTCCTATGATGAGAAACTTGCTGATGTTTTGGGTAAGAGAGGAGATGTTTCTTCGGCACTAGATACTTTTAGCTTAACAGCACTGGATCAGCTTTCTGGAAGCTATTTTTCCTACTTTATGACGCCAGCTGAAAATTTTTCCAAAAAGCTTGTTGAAAATTTGAACCATCAATCTGCAGAAGGAGTCGCTTATGTCGTAAATAGTGTAGAATATGCAAAAAAACTGAAAGAAATGGGGGTTCGTTTTTTGATGACGGATGAAGTCGCAAAACTTAAGGAGGGCTTGAAATAA", "aa": "MIYHGPKIYPHTTCKQETREICQMTREELQKCKLKNGEDILLLEEFLPQVKNRFSFFFLDFKTSTNPQCKQNNQLLLDKAIALVKKYKMDGNTIFSSYDEKLADVLGKRGDVSSALDTFSLTALDQLSGSYFSYFMTPAENFSKKLVENLNHQSAEGVAYVVNSVEYAKKLKEMGVRFLMTDEVAKLKEGLK", "aa_hexdigest": "ed211961a2efeacc8d8eaac018920f7b", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 22009.119799999993, "isoelectric_point": 8.227149009704593 }, "id": "NKOCPLAKPK_1484", "locus": "GCA003260355_01451" }, { "type": "cds", "sequence": "contig_1", "start": 785621, "stop": 785776, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAATTATTGATGGGGCGTCTTATTTCCCTGTGGATGAAATTTTGAATTACCTTAGACTCTCAATTGCCTACCAGATGAAAGCTCACGGAATAAAGGAAGGAGATATTTATGCCAATACGGCTGACAAAACCTATATCATTTCTCACAGATGA", "aa": "MEIIDGASYFPVDEILNYLRLSIAYQMKAHGIKEGDIYANTADKTYIISHR", "aa_hexdigest": "f95acaa551fc0f5e8a752d91549bee7c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5849.6029, "isoelectric_point": 5.425247764587401 }, "id": "NKOCPLAKPK_1485", "locus": "GCA003260355_01452" }, { "type": "cds", "sequence": "contig_1", "start": 785999, "stop": 786547, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAACCGGTAGTCTTTGACTACGCTACAGGGACAAGGTATCAAAACTATACGGGATATGTCGCACCTTTGGCGGAGTTTTTTAATCGCCTTGGCTATGCAACGACCTTTCTTAGCACGGTAAGCCTCGAGTTTTTGGATCAGAGAAATTTCCTCAAAAGAGCCGGCTACCAAACCATTATCGGAGAGGAAGCTTTTGAAGACAGGAAAAAATATACCTTTGATGCGGCACCTGACAAAGACTTATATGACAAGGCTTTAGAAATAGTAAAAGAGCAAAAATCTCCCTTCTTCCTTACCTTACAGACGATTTCTTCACATACTCCTTATAATACTCCCTATGGACGCACAGCAGAAGCAATGTATCGTTATGAAGATGAAACTTTTTCAGAATTTTATCAAGAGCTCAAAAAAACTGATTTTTTCAAAGATGGAATACTTATGGTTGTAGGAGATCATCGTAAAATGGTACCTCTTGAGGACGAGGAGTTTGCCAAGCGAGGAACGACAGCTGCTGCTAAAATTATGGCTTTTGCTATAGGATCTTGA", "aa": "MEPVVFDYATGTRYQNYTGYVAPLAEFFNRLGYATTFLSTVSLEFLDQRNFLKRAGYQTIIGEEAFEDRKKYTFDAAPDKDLYDKALEIVKEQKSPFFLTLQTISSHTPYNTPYGRTAEAMYRYEDETFSEFYQELKKTDFFKDGILMVVGDHRKMVPLEDEEFAKRGTTAAAKIMAFAIGS", "aa_hexdigest": "1a4cfd24d3b9960fffecb9dc8f118d83", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 21011.426999999985, "isoelectric_point": 5.062956047058105 }, "id": "NKOCPLAKPK_1486", "locus": "GCA003260355_01453" }, { "type": "cds", "sequence": "contig_1", "start": 786548, "stop": 786649, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCTCTAACCGATAAAGTGAGCGATGATGGAGTTTCTTTCCTCCAGATGCATGCTAATGGAACTTCTTCAGATATGGGGCACATTGCAACCCTGCTCTGA", "aa": "MPLTDKVSDDGVSFLQMHANGTSSDMGHIATLL", "aa_hexdigest": "13a8eb1c51f7fe8d87d84003a403c3b1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3489.9051999999992, "isoelectric_point": 4.538673210144044 }, "id": "NKOCPLAKPK_1487", "locus": "GCA003260355_01454" }, { "type": "cds", "sequence": "contig_1", "start": 786830, "stop": 787516, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTAATTCTGAGTTTCCAACAGAGATTGAGTTTTACGACAGGTCTAAATTTCCTTTCTGCAGGTCCGAATGTAGTATTTCTGTATAGTATGATTTTTTTGATGAGCTTTTTGCTCGTGGTATTCCTAGCATTTCTCCTAGCAAAAAGTATCAAAATAAAGATCAATGAAAATTTATTTCTCAGAGTTATGATCGCATCATTTTTGCGGTATGGAATACCTTTTCCTCAGATATCATTCTTGGGAGTAGAGTTGCCAGAAAAAAACATTCTAGATAAAGTTGCCAGAAACGCTTATGAACTCTCATTGCAAGATCAAAAGGAAAAATCTGACCAATCCACCAAGAAAAAGCTCGATCTTCCAGAGATAAAGTTAGAAAACCTCGAAAAAAGAAAAAGTTTCTGGCTTGAAATTGCAAAGAAACAGCCCGAGGAAATTTTCCAGACTTTTGGCTATTCTTCACTCAAAAAAGCAAAAGACCAATACGGAGATTCCTATGTTTTTGAAGTACTCTCTGCAGCGGTAGCAGCGGCTCCAGAGTTGGCGTTTAGCTATATCGATGTCTATAAAGATCGAAAAGATGAGCAGGGAGATACCGTTGTAAAGCCTTTGCTCAAGATCGCTATTCAACAAAAGCCAGAGCTCGCGTTTCGTTACCTTGATAAATATCAGGATATTGTAGAGTGA", "aa": "MVILSFQQRLSFTTGLNFLSAGPNVVFLYSMIFLMSFLLVVFLAFLLAKSIKIKINENLFLRVMIASFLRYGIPFPQISFLGVELPEKNILDKVARNAYELSLQDQKEKSDQSTKKKLDLPEIKLENLEKRKSFWLEIAKKQPEEIFQTFGYSSLKKAKDQYGDSYVFEVLSAAVAAAPELAFSYIDVYKDRKDEQGDTVVKPLLKIAIQQKPELAFRYLDKYQDIVE", "aa_hexdigest": "bd370b3ad8c36a530cd33df5179bdfe0", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 26292.4234, "isoelectric_point": 8.79872570037842 }, "id": "NKOCPLAKPK_1488", "locus": "GCA003260355_01455" }, { "type": "cds", "sequence": "contig_1", "start": 787544, "stop": 787813, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCCAAAAAAATAAAAAAATCAGATATCTTCCTCAAAAAGATTGTTTTCATCGCTTTCATCTTGCTTATCGTGATCAAAGTCCTACTCATCACGGTTGCTTATTCCTACGTCCTTCAAAAGATGGGAGTATTGCATATGCTATACTACTTTTTTACTTTGCTGCAGTCAGATTTCCTTATTTTAGGGATAGCACTTGCTCTAATTCGGGCTGTAGACAAGCAAAAATACCTCATATTAAAAATACTCTTTCTCCTTGGGGTAATTTGA", "aa": "MPKKIKKSDIFLKKIVFIAFILLIVIKVLLITVAYSYVLQKMGVLHMLYYFFTLLQSDFLILGIALALIRAVDKQKYLILKILFLLGVI", "aa_hexdigest": "ee16834a48bbb918d4489f7603dcb01f", "start_type": "ATG", "rbs_motif": "TTATT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10304.903099999989, "isoelectric_point": 9.999062538146973 }, "id": "NKOCPLAKPK_1489", "locus": "GCA003260355_01456" }, { "type": "cds", "sequence": "contig_1", "start": 787849, "stop": 788070, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTCTCTCCAAAAGAAACTATCCATCCTGGAATGCAGGTGAACTATATGACTCCAAATGATTTAGGCGTTTTGACGATCACGGAAATACTAGATCAACAAGGGCGTAGTATGGATAAAGCTGATTGTAATAGTGATTCGGTGTTTCTAAAAGCTAGTATCCCGCTTGAAACAGGGATGATTCTTTATCTTCCTCCATCTATTCCTCTGGACAAGAATTAG", "aa": "MLSPKETIHPGMQVNYMTPNDLGVLTITEILDQQGRSMDKADCNSDSVFLKASIPLETGMILYLPPSIPLDKN", "aa_hexdigest": "2c23e9f3111e1ae4b1ea8714e5f71523", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8048.244699999996, "isoelectric_point": 4.510878944396972 }, "id": "NKOCPLAKPK_1490", "locus": "GCA003260355_01457" }, { "type": "cds", "sequence": "contig_1", "start": 788641, "stop": 788985, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATATCTTTCCAAGAAATGTAGATATTAAGATTTTTGAGGCGGTAGTTGAGAGGATTGCGGATCTTAATGCTGATGGTATTATTTTCTCAGATCCAGGAACTTTTCATATTCTGAGAAAATACGCCAAAGATACTCCTTTACATCTTAGTACCCAGACAACCACGCTAAATTATTCTGCGGTTCAGTTCTGGAGAGATATCGGGGTAAAAAGAATCGTCCTCGCACGTGAATTGCATATTGATGAAGTCAAAGAAATCAAAGAAAAAGTTCCAGAGATGGAGCTCGAAATTTTTGTGCAGGGGGCAATGTGTATGACCTATTCAGGCAGATGTCTTTTGTGA", "aa": "MNIFPRNVDIKIFEAVVERIADLNADGIIFSDPGTFHILRKYAKDTPLHLSTQTTTLNYSAVQFWRDIGVKRIVLARELHIDEVKEIKEKVPEMELEIFVQGAMCMTYSGRCLL", "aa_hexdigest": "6b6fb0f70ab4df8ad88b0211072f4df2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13127.162399999994, "isoelectric_point": 5.890020179748535 }, "id": "NKOCPLAKPK_1491", "locus": "GCA003260355_01458" }, { "type": "cds", "sequence": "contig_1", "start": 789004, "stop": 789183, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCATATTCCAGCAATCGTAGCTCCTGGAGGTTCTTTTCAAAAAGCTGTTACTGCAGCTAGATATGGAGCAGATGAAGTATATATGGGAGTTCCTTTTACCTCCCTTCGTATGAGATCAAATAAAGTAAGAGATTTTGAAAAACTTAAGGAATCAGTAGATGCTGTCCATGCCTTATGA", "aa": "MHIPAIVAPGGSFQKAVTAARYGADEVYMGVPFTSLRMRSNKVRDFEKLKESVDAVHAL", "aa_hexdigest": "1909a46a3dc8d5c11992e22d96c34ea7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6464.413700000001, "isoelectric_point": 9.300031852722167 }, "id": "NKOCPLAKPK_1492", "locus": "GCA003260355_01459" }, { "type": "cds", "sequence": "contig_1", "start": 789494, "stop": 789991, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7W4E3X5", "UniRef:UniRef90_A0A328EHR2" ], "nt": "GTGCAAACCCAAGCAAAACAGGCAAAGCAAGTTGCCCAGCAAATCAAAAAAGACAAGGCGATCAATCATCATCTTGCACAGTTTTTGGCATTTTTGATCAAGGAAATAAAAAATGAAAATCTGATTACTGCTATCACCAATACCTTTTTCAAGACAACAAATCCAAAAGACCAGGTTACATACCTTAGGAAAGATGTTAATACTTATGTAATAGTTGGTTTTTTTATTCCTTTTTTTGCCCAAGAAGCAGAGAAGGCTAAAATTGTAACTTTCTATGAAGCGTTAGGATGGAGAGAAGCAGCAAACGGGCTGAGGGAATATGTACAATATTTAGGAAAACTCTCTGAGGTATATCATGATAATGTACCTATTAACCAGGAAAGCTTGTTAGAGCTTATCGCAATGATCGCTCAAGAGCGACTTTCCAGCGAAACCAAGCATCTTTCAGAGGAGGAATCTAAAAAAAGAATCTTAACTATTTTGAATAGCGAAGTTTAA", "aa": "MQTQAKQAKQVAQQIKKDKAINHHLAQFLAFLIKEIKNENLITAITNTFFKTTNPKDQVTYLRKDVNTYVIVGFFIPFFAQEAEKAKIVTFYEALGWREAANGLREYVQYLGKLSEVYHDNVPINQESLLELIAMIAQERLSSETKHLSEEESKKRILTILNSEV", "aa_hexdigest": "b61b331f714392d91a3624aa3c1984ec", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A328EHR2", "query_cov": 1.0, "subject_cov": 0.8549222797927462, "identity": 0.982, "score": 305.0, "evalue": 2.99e-104, "valid": true, "uniref50_id": "UniRef50_A0A7W4E3X5" }, "pscc": { "uniref50_id": "UniRef50_A0A7W4E3X5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7W4E3X5" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19048.654400000014, "isoelectric_point": 7.889076805114746 }, "id": "NKOCPLAKPK_1493", "locus": "GCA003260355_01460" }, { "type": "tRNA", "sequence": "contig_1", "start": 790178, "stop": 790264, "strand": "+", "gene": "trnS", "product": "tRNA-Ser(gct)", "amino_acid": "Ser", "anti_codon": "gct", "score": 71.8, "nt": "GGTGAGGTGGATGAGTGGTCGAAATCAGCTCACTGCTAACGAGTCAGGCGAGAGATCGCCTCGAGGGTTCGAATCCCTCCCTCACCG", "db_xrefs": [ "SO:0000269" ], "anti_codon_pos": [ 790205, 790207 ], "id": "NKOCPLAKPK_13", "locus": "GCA003260355_01461" }, { "type": "tRNA", "sequence": "contig_1", "start": 790278, "stop": 790351, "strand": "+", "gene": "trnM", "product": "tRNA-Met(cat)", "amino_acid": "Met", "anti_codon": "cat", "score": 59.0, "nt": "GGCACTATAGCTCAGCAGGTTAGAGCGCGCCTCTCATAAGGGCGAGGTCGGTGGTTCGAGTCTACCTAGTGCCA", "db_xrefs": [ "SO:0000266" ], "anti_codon_pos": [ 790312, 790314 ], "id": "NKOCPLAKPK_14", "locus": "GCA003260355_01462" }, { "type": "cds", "sequence": "contig_1", "start": 790470, "stop": 790730, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATTCCAGTCTACTCTAATGCCGATTATTGTGATCATCTCAAAAGTCATTCAAAATATCTTTTATTTATCGAGTACATTATTATGGCCGCTAAGAAGAAAGGAGGAGTTGTTACTGTTGCAATGGTATGTAGTGAATGTAAAGCTACGAACTACTTTACAAGCATCAATAAGGCTACAACTCCAAAGCTTGCTCAGACAAAATACTGCAAGCACTGTAAAAAACACACAGAACACACTTCAAAGGAAAAATTAAAGTAA", "aa": "MIPVYSNADYCDHLKSHSKYLLFIEYIIMAAKKKGGVVTVAMVCSECKATNYFTSINKATTPKLAQTKYCKHCKKHTEHTSKEKLK", "aa_hexdigest": "96f98957a45bd8957b647d807af8b413", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9790.457699999997, "isoelectric_point": 9.42091007232666 }, "id": "NKOCPLAKPK_1494", "locus": "GCA003260355_01463" }, { "type": "cds", "sequence": "contig_1", "start": 790773, "stop": 791069, "strand": "+", "frame": 3, "gene": "rpsP", "product": "30S ribosomal protein S16", "db_xrefs": [ "GO:0000314", "GO:0003735", "GO:0006412", "GO:0022627", "SO:0001217", "UniParc:UPI000DBF8210", "UniRef:UniRef100_A0A328EHJ1", "UniRef:UniRef50_A0A328EHJ1", "UniRef:UniRef90_A0A328EHJ1" ], "nt": "ATGTTAACAATTAGATTGTCAAGACACGGAAGATCAAAAAGACCTTTTTATAGAATCGTTTTGACCGAACATACTAAACCAGCAAAATGTGGGTACAGAGAAGTCCTTGGATCTTATGATCCTCTTCATCATAAGCTTGAAGCGAATGTTGGGGCTGTTAAAGAGCGAGTTTCAAAAGGTTCTCAGATGTCTGAAAGAGTGGCAAAGCTTCTTTATAAAGAGACAAATGATAAGCTTTTCCAGAAGTTCTTTGAAATGAAGGATAAGAAAAGATTACCAAAGAATGCAGATAAGTAA", "aa": "MLTIRLSRHGRSKRPFYRIVLTEHTKPAKCGYREVLGSYDPLHHKLEANVGAVKERVSKGSQMSERVAKLLYKETNDKLFQKFFEMKDKKRLPKNADK", "aa_hexdigest": "1da9492518605a574064549621131590", "start_type": "ATG", "rbs_motif": "TTATT", "ups": { "uniparc_id": "UPI000DBF8210", "uniref100_id": "UniRef100_A0A328EHJ1", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBF8210", "UniRef:UniRef100_A0A328EHJ1" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EHJ1", "uniref90_id": "UniRef90_A0A328EHJ1" }, "psc": { "uniref90_id": "UniRef90_A0A328EHJ1", "gene": "rpsP", "product": "30S ribosomal protein S16", "uniref50_id": "UniRef50_A0A328EHJ1", "go_ids": [ "GO:0000314", "GO:0003735", "GO:0006412", "GO:0022627" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EHJ1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EHJ1" ], "product": "Small ribosomal subunit protein bS16" }, "genes": [ "rpsP" ], "id": "NKOCPLAKPK_1495", "locus": "GCA003260355_01464" }, { "type": "cds", "sequence": "contig_1", "start": 791419, "stop": 791550, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGGAAAATTATTTCTTATATTGGAGGACTAGGGAATATCATTAGAAAGTCTCAAACTACTAATGAATATAATGGAGATCTCTTCTACGATCTTAATAGTCCGACTTCTCTTGATAGTAGCAGTTTGTAA", "aa": "MGKIISYIGGLGNIIRKSQTTNEYNGDLFYDLNSPTSLDSSSL", "aa_hexdigest": "1d530799a50797a9af6c68b2465b1681", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4684.1513, "isoelectric_point": 4.682589530944824 }, "id": "NKOCPLAKPK_1496", "locus": "GCA003260355_01465" }, { "type": "cds", "sequence": "contig_1", "start": 791975, "stop": 792109, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAGAACTCAGACCGTTGGTGACTTTTCTCGCCAACTTACTCTGGAGAAACCAGGGACTATTTTGTCCAGTCAGGATATTCAGGTAACTGCTCAGGCTAATGGGAAAGTTGCCCGTATTCTTTCTAGGGAATGA", "aa": "MRTQTVGDFSRQLTLEKPGTILSSQDIQVTAQANGKVARILSRE", "aa_hexdigest": "eb98c78be18785abe68c3943bbab26d0", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4845.4516, "isoelectric_point": 9.96889133453369 }, "id": "NKOCPLAKPK_1497", "locus": "GCA003260355_01466" }, { "type": "cds", "sequence": "contig_1", "start": 792830, "stop": 793447, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00364.28", "PFAM:PF13533.12" ], "nt": "GTGGAGGGACAGCTTAACGGATCTTCTACTATGGATAAAGAAAAAGCTGACCTTAACTATAGTTCTACCTTGGCTAATAGTCAGAATTCTATCTTTTCTGCTGAAATCGCACTGAAAAATGCAAAAGTTAACTACGAAACTCTCCTTGCAAATAAAGATATTCAGCTTGACCTGCTTAATAATGCTATCACTGATGCGAAAATTGCCTATGAATCGGCACTGATCCAGTATAATAAATTGCAAGTAAGGTCGCCTGTGTCTGGAGTTATTGGAACGATTCTCGTAAGTGAAGGACAAGAAGTTGGAGCAGGAACACCAATCTTTAAGGTTTCAGGGACGAAGAAGCAGCAGATTGAAGTTTATCTCACTGCTGAAGAATATCACTATCTCTCTGAAAAAGAACCGGTTCTTATTAGCTATCAAAATCAGCTCCTTACTGGACTTATTGATGCAATGAGTTCTGTAGCTGATAGGACAAATCTCTTTAAAGCGACTATCCAGCTCGCTAGTGAAGTAGCTCTTGTTGGTGATGTAGCGAAAGTTAGCTTTCCACTTAAGGTCGCAGAAAATACGCTCCTCCCTCTGGATATGGTCAAAGTGCTTAATGAAAATGTATGA", "aa": "MEGQLNGSSTMDKEKADLNYSSTLANSQNSIFSAEIALKNAKVNYETLLANKDIQLDLLNNAITDAKIAYESALIQYNKLQVRSPVSGVIGTILVSEGQEVGAGTPIFKVSGTKKQQIEVYLTAEEYHYLSEKEPVLISYQNQLLTGLIDAMSSVADRTNLFKATIQLASEVALVGDVAKVSFPLKVAENTLLPLDMVKVLNENV", "aa_hexdigest": "125200fcefa936b76f106b99de62ad5a", "start_type": "GTG", "rbs_motif": "TTCTT", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00364.28", "name": "Biotin_lipoyl", "length": 29, "aa_cov": 0.14146341463414633, "hmm_cov": 1.0, "evalue": 2.9237039099204387e-06, "score": 23.51372718811035, "start": 81, "stop": 109 }, { "id": "PF13533.12", "name": "Biotin_lipoyl_2", "length": 37, "aa_cov": 0.18048780487804877, "hmm_cov": 1.0, "evalue": 1.0527655364083461e-06, "score": 24.834829330444336, "start": 80, "stop": 116 } ], "seq_stats": { "molecular_weight": 22287.143800000013, "isoelectric_point": 4.797574806213379 }, "id": "NKOCPLAKPK_1498", "locus": "GCA003260355_01467" }, { "type": "cds", "sequence": "contig_1", "start": 793644, "stop": 794111, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7W4E2U2", "UniRef:UniRef90_A0A660MUH5" ], "nt": "TTGTATATCAAGGAAAATTACTGTAAGGTTGTCGCAGTAGAAGATGGTTTTTACAAGCAGATTGAGCATATCAACTTTGGAATCAACTTTTTAATGCAGATGTACAAGGACAATCAGATCGTGAAATACCGATACAAGAGTGCAGAGGAAGTCGAGCATAACCCTCTTGCCAAGAATCTCGTCTTAGAAAGTATTAGATTTTATGTAGAATACCTGTGTAAATGGCTCAACGATCTTCATCTCACGAATAAAGATATTTTTCTGGTTTCTCCTATTGTTAAAAATGGAAACTTCATGGAGGAGTTTAATAAATCCTATACGAGTTTCCACAATAAATATATTGTTCCTTTTCATCATTCTAGTCAGCTAGAGCTTTTTGGAAAGCATCGAGCTCCTGAAGATATGGATACAGTAATTTTCCTTAATGGTGCAAAGATTAAAAATCAGATTATCGGAGAGAGAAAGTAG", "aa": "MYIKENYCKVVAVEDGFYKQIEHINFGINFLMQMYKDNQIVKYRYKSAEEVEHNPLAKNLVLESIRFYVEYLCKWLNDLHLTNKDIFLVSPIVKNGNFMEEFNKSYTSFHNKYIVPFHHSSQLELFGKHRAPEDMDTVIFLNGAKIKNQIIGERK", "aa_hexdigest": "76a299712c86a05cc25de767fed8a578", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 793644, "stop": 794411, "observations": { "mutation": [ 794291 ], "stop": [ 794291 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A660MUH5", "query_cov": 1.0, "subject_cov": 0.46546546546546547, "identity": 0.9940000000000001, "score": 320.0, "evalue": 3.81e-108, "gene_start": 1, "gene_end": 155, "reference_start": 179, "reference_end": 333, "reference_sequence": "MFYLWREAYDPKYPKFCVVKFQERLGMQQYDRYESLAEIIDDSSRHSQYEHHFLLDDGRFHFFRFDIYKETESVFTIEDLNAVIEERLEYLRNITKEELLFTNIDTIFVDGEPQKFVIGSKGKLFFRLYFIYINRNTLLEFNKAYGNVFNQKNLHLRPESFKTISFLKRKLGRDAFLLLYIKENYCKVVAVEDGFYKQIEHINFGINFLMQMYKDNQIVKYRYKSAEEVEHNPLAKNLVLESIRFYVEYLCKWLNDLHLTNKDIFLVSPIVKNGNFMEEFNKSYTSFHNKYIVPFHHSSQLELFGKHRAPEDMDTVIFLNGAKIKNQIIGERK" }, "paralog": false, "identity": 0.9921568627450981, "score": 514.0, "evalue": 3.85e-187, "description": "Internal stop codon at 794291. Nonsense mutation around 794291.", "psc": { "uniref90_id": "UniRef90_A0A660MUH5", "uniref50_id": "UniRef50_A0A7W4E2U2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A660MUH5", "UniRef:UniRef50_A0A7W4E2U2" ] }, "pscc": { "uniref50_id": "UniRef50_A0A7W4E2U2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7W4E2U2" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 18478.0902, "isoelectric_point": 7.77059497833252 }, "id": "NKOCPLAKPK_1499", "locus": "GCA003260355_01468" }, { "type": "cds", "sequence": "contig_1", "start": 794181, "stop": 794348, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGAGATTCTTTTGGTTAAAGACATTTCCATACGCCTTATTAAATTCGAGAAGGGTATTTCTATTGATATAGATGAAGTAAAGACGGAAGAAAAGCTTTCCTTTGGCTCAGATGACGAATTTCTGAGGCTCTCCATCCACAAAAATTGTATCAATATTGGTAAATAG", "aa": "MEILLVKDISIRLIKFEKGISIDIDEVKTEEKLSFGSDDEFLRLSIHKNCINIGK", "aa_hexdigest": "d10705f606d609bce977ee5bf2e8cb09", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6335.346499999998, "isoelectric_point": 5.236030769348144 }, "id": "NKOCPLAKPK_1500", "locus": "GCA003260355_01469" }, { "type": "cds", "sequence": "contig_1", "start": 794289, "stop": 794645, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTTATCTCTGGAGAGAAGCTTACGATCCGAAGTATCCCAAGTTCTGCGTGGTGAAATTTCAAGAAAGGTTGGGGATGCAGCAGTATGATAGGTATGAATCTCTCGCTGAGATTATTGATGATTCTTCACGCCACTCTCAGTATGAGCATCATTTCCTCCTCGATGACGGGAGGTTTCATTTCTTTCGTTTCGATATCTACAAAGAGACGGAAAGTGTTTTTACGATAGAAGATTTAAATGCGGTTATTGAGGAGAGACTGGAATACCTCAGAAATATTACCAAAGAGGAGCTGCTATTTACCAATATTGATACAATTTTTGTGGATGGAGAGCCTCAGAAATTCGTCATCTGA", "aa": "MFYLWREAYDPKYPKFCVVKFQERLGMQQYDRYESLAEIIDDSSRHSQYEHHFLLDDGRFHFFRFDIYKETESVFTIEDLNAVIEERLEYLRNITKEELLFTNIDTIFVDGEPQKFVI", "aa_hexdigest": "7208f25a5bb18d27a2b67adbf3c3aad4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14511.151299999981, "isoelectric_point": 4.765460777282715 }, "id": "NKOCPLAKPK_1501", "locus": "GCA003260355_01470" }, { "type": "cds", "sequence": "contig_1", "start": 794809, "stop": 795414, "strand": "-", "frame": 1, "gene": null, "product": "Peptidase M48 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4IJ67", "UniRef:UniRef90_A0A328EKM6" ], "nt": "ATGATTGAAAACCTCTGCATCTCCAAAGGATTGCCAACACCGAGGATTGGGATTATTGAGACACCAGGAATGAATGCCTTTGCTCTAGGCTGGAGACCAAAAGATTCTTGGATTGTGTTTACAAGAGGCTTGCTCAATGCACTAGAAAAGTCTGAAATTGAAGCCGTTGCAGGCCACGAACTCACGCATATTATCAACAAGGATTCACTCTTGATGCTCGTGATGGTGCTTTATATTGGAGCGATAAGTTTATTGGGAGAGATCCTGCTAAGGAGTGGAGGACGCGGCGGCAACTCCAAAAACAAGAACGTATTACCACTTATTGGTCTCGTTTTGCTGATGCTCGGCTACCTTTTCTATCCGCTTGTGAAGCTTGCGATTTCGAGGAAGAGAGAGTATCTCGCTGATGCGGGAAGTGTTGAGCTTACCAAGGACAATCAAGCAATGATTTCTGCACTTAGAAAGATTTCCGCACGCCCAGAAGTCAAGCTTGACAACAAAGAAATGGCAGCAATGTTTATCGCAAACCCTTTCAAGAAGATGAGTTCGCTCTTTCAAACTCATCCAAGCATAGAAGATAGAATAAAAGCGCTAGAGCATTATTAA", "aa": "MIENLCISKGLPTPRIGIIETPGMNAFALGWRPKDSWIVFTRGLLNALEKSEIEAVAGHELTHIINKDSLLMLVMVLYIGAISLLGEILLRSGGRGGNSKNKNVLPLIGLVLLMLGYLFYPLVKLAISRKREYLADAGSVELTKDNQAMISALRKISARPEVKLDNKEMAAMFIANPFKKMSSLFQTHPSIEDRIKALEHY", "aa_hexdigest": "32a2461d0e743f9e2b2a69bf68559645", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 794809, "stop": 795714, "observations": { "mutation": [ 795459, 795705 ], "stop": [ 795459, 795705 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EKM6", "query_cov": 1.0, "subject_cov": 0.6421725239616614, "identity": 0.99, "score": 385.0, "evalue": 2.5e-133, "gene_start": 1, "gene_end": 201, "reference_start": 113, "reference_end": 313, "reference_sequence": "MQGLQHAIRSNRMKTGLLIVLFPLMLGVALFFVFLFASNGTTYRESRGVRVASLASEERMQEALTETGAALALLAPVLMIWLLISFFFQRQLMFSFSGAKPVTRAQEPKLYNMIENLCISRGLPTPRIGIIETPGMNAFALGWRPKDSWIVFTRGLLNALEKSEIEAVAGHELTHIINKDSLLMLVMVLYIGAISLLGEILLRSGGRGGNSKNKNVLPLIGLVLLILGYLFYPLVKLAISRKREYLADAGSVELTKDNQAMISALRKISARPEVKLDNKEMAAMFIANPFKKMSSLFQTHPSIEDRIKALEHY" }, "paralog": false, "identity": 0.9800664451827242, "score": 570.0, "evalue": 1.32e-208, "description": "Internal stop codon at 795459, 795705. Nonsense mutation around 795459, 795705.", "psc": { "uniref90_id": "UniRef90_A0A328EKM6", "product": "Peptidase M48 domain-containing protein", "uniref50_id": "UniRef50_A0A2W4IJ67", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EKM6", "UniRef:UniRef50_A0A2W4IJ67" ] }, "pscc": { "uniref50_id": "UniRef50_A0A2W4IJ67", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4IJ67" ], "product": "Peptidase M48 domain-containing protein" } }, "id": "NKOCPLAKPK_1502", "locus": "GCA003260355_01471" }, { "type": "cds", "sequence": "contig_1", "start": 795457, "stop": 795678, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTCGGAGTTGCGCTCTTTTTCGTCTTTCTCTTTGCTTCAAACGGGACGACGTATAGGGATAGCAGAGGAGTGAGGGTCGCAAGCCTTGCTTCCGAAGAGAGAATGCAAGACGCACTAACAGAAACGGGAGCAGCCTTAGCTTTACTTGCTCCAGTCTTGATGATTTGGCTCTTAATCTCCTTCTTTTTCCAGAGGCAGCTCATGTTTTCCTTCTCTTGA", "aa": "MLGVALFFVFLFASNGTTYRDSRGVRVASLASEERMQDALTETGAALALLAPVLMIWLLISFFFQRQLMFSFS", "aa_hexdigest": "8b14c7fa0ad008c1a1bbcdb7d670e01d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8172.521699999996, "isoelectric_point": 6.039393043518066 }, "id": "NKOCPLAKPK_1503", "locus": "GCA003260355_01472" }, { "type": "cds", "sequence": "contig_1", "start": 795711, "stop": 796157, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAGAGTCAAAAATGGAGCCCATTTTTCACGCTCAGGGGATCGGGCAAGGAAAGCTACCCGCAAATGCGTTGCAGTTGGTCATTCTAAGGAACGAAGCCGCCCTCATTTCGATACTTCTAAAAGCAAAATAACCCTATTAGGCATGACCTCATATCCTTCACTTCACTCAGCAACAAAAAATTATCTTCAGGAAGGCGAGTTCCTCTTAGTAGCCAAGCCATCTTGCACTCAAAAGGGCACTCCTTTTCTCATCGCTCTTTCATTTACCATTCTGGATCACCTTCCAAACCAACAAAAGAGTCAGATTTTTTTCCTCATTCCTACCGCTTCTTCAAAAAATCCTTTGAAAATTCACCGCATAACCATAAGATACACATCAGATTTATCTCTTTTTCACTCTTATTCATGCAAGGACTTCAACACGCAATCCGAAGCAATCGTATGA", "aa": "MRVKNGAHFSRSGDRARKATRKCVAVGHSKERSRPHFDTSKSKITLLGMTSYPSLHSATKNYLQEGEFLLVAKPSCTQKGTPFLIALSFTILDHLPNQQKSQIFFLIPTASSKNPLKIHRITIRYTSDLSLFHSYSCKDFNTQSEAIV", "aa_hexdigest": "b242b08cbbca1ebe42b91b6a0bdd3b18", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16657.971099999984, "isoelectric_point": 10.065078163146971 }, "id": "NKOCPLAKPK_1504", "locus": "GCA003260355_01473" }, { "type": "cds", "sequence": "contig_1", "start": 796522, "stop": 796911, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAACTTGCGAATCCCAGAATTCCTATCCTGAGAGGAGGGAGAGGAGGAGCTGCTTTTCACTTCCAAGCTTCCAAAACCTCCGACATTTACTCTTGTTCCCATTCCCGACCTGCTCGAGTCCCAAATCCTGCCCCCTTGCTGAGGCTGTCCAGCAGGTCAATGACCTTATTTTCAAAAAATATTGGGGTTCCGATCCCCTCGACTATCAAATAGTGAGTAAAGGTTTTTGGGGTAAAACAGGTAAAAAGTCTGATTCGACCCCCAAGATTGCTCCTCCGGGAGGTCGCTGGTCCAATCTGACCCCCCAAATTAGCCTTCCGGGAGGTCGCTGGTCCAATTTGACCCCCCAAATTAGCCTCCCCGGGAGGTCGATGATACAAGAATGA", "aa": "MKTCESQNSYPERRERRSCFSLPSFQNLRHLLLFPFPTCSSPKSCPLAEAVQQVNDLIFKKYWGSDPLDYQIVSKGFWGKTGKKSDSTPKIAPPGGRWSNLTPQISLPGGRWSNLTPQISLPGRSMIQE", "aa_hexdigest": "56ff30c71312f73e658c386ed50d59d4", "start_type": "TTG", "rbs_motif": "TTCTT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14559.498199999984, "isoelectric_point": 9.64912815093994 }, "id": "NKOCPLAKPK_1505", "locus": "GCA003260355_01474" }, { "type": "cds", "sequence": "contig_1", "start": 797021, "stop": 797884, "strand": "+", "frame": 2, "gene": null, "product": "Alanyl-transfer RNA synthetases family profile domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7W4HH44", "UniRef:UniRef90_A0A328EHG0" ], "nt": "ATGAAATTGAGTGTAAATAAAGCTCAGAGATATGCAAAGATGAGAGCTCACACGGCAACGCATCTGCTCCATGCTGAGCTTGGAAAGATTTTTCCTGACACGAAACAGGCAGGGTCTTTGGTAGATGATGATTTGTTGAGATTTGATTTTTATGCGGGAAGGCTCTTGAATCCTGAGGAGATTTTGAAGATCGAGCAACGTATCAATCAGATGATTGCTTTAGCGTGTCAGGTCGAGACGGTAGAGCTTTCGATTGAAGAGGCTTCTCAGTTGGGCGCAAAGATGTTCTTTGAAGAAAAGTATGGAGACGTCGTGCGTGTGGTGAAAGTGCTTAACGGAACTAACCAGACTCAAAAGGCTGACAGCCAAGAAGGGTCAGATTTTTCTGATTATGGGGAGTTTTTGTCCTTGGAGTTTTGTGGGGGGACGCACGTGAAGAATACTAGAGAAATCGGGGCGTTTACGATCATTTCTCAGGAAGCGGTGGCCTCTGGAGTGAAGAGAATTGTCGCGCTTACGGGACCAAGAGTGCTCGAGAAGATCGAACAGCAACAAGCCTTGTTGACCCAGCTCGGGGAGCTTTTGGAGGTGAAGTCGTTTGCGCAGTTGCCGGAGAGAGCGGCGAAATTTTTGAAGGATTTTGGGGAGATGAAAGCTCAGCTAGGAGCGGTTGAGACGAAGATGATCCATGCGTTATTGATTTCTGGCGACAAAAAGTCAAATCCTGACTTTGATGTCATTATGAAAGTGCCTGTTGAGACAAATTTTAAATTGCTCGGGACGGAAGCTAAGCAGGTTTTCGCTGGCAAGAACGTGCTCTTGGCGACGACTGCTGGGAATTTTTTGCTTTTCACGCAGCCTTGA", "aa": "MKLSVNKAQRYAKMRAHTATHLLHAELGKIFPDTKQAGSLVDDDLLRFDFYAGRLLNPEEILKIEQRINQMIALACQVETVELSIEEASQLGAKMFFEEKYGDVVRVVKVLNGTNQTQKADSQEGSDFSDYGEFLSLEFCGGTHVKNTREIGAFTIISQEAVASGVKRIVALTGPRVLEKIEQQQALLTQLGELLEVKSFAQLPERAAKFLKDFGEMKAQLGAVETKMIHALLISGDKKSNPDFDVIMKVPVETNFKLLGTEAKQVFAGKNVLLATTAGNFLLFTQP", "aa_hexdigest": "a041cbc458bb5e37e17579013a5ca59b", "start_type": "ATG", "rbs_motif": "TTCTT", "psc": { "uniref90_id": "UniRef90_A0A328EHG0", "query_cov": 1.0, "subject_cov": 0.896875, "identity": 1.0, "score": 514.0, "evalue": 4.96e-183, "valid": true, "product": "Alanyl-transfer RNA synthetases family profile domain-containing protein", "uniref50_id": "UniRef50_A0A7W4HH44" }, "pscc": { "uniref50_id": "UniRef50_A0A7W4HH44", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7W4HH44" ], "product": "Alanyl-transfer RNA synthetases family profile domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_1506", "locus": "GCA003260355_01475" }, { "type": "cds", "sequence": "contig_1", "start": 798131, "stop": 798679, "strand": "-", "frame": 3, "gene": null, "product": "Peptidase M43 pregnancy-associated plasma-A domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7W4E340", "UniRef:UniRef90_A0A660MTJ4" ], "nt": "ATGCAAGGCGATCCGCTTCACTCCGTTTTCAAGACCTTGATCATCAACGGTGAAACCGGTAAGAGAAGAGGAGGAGCCACCCGAAAAAGAATCACCATCAATCTCTCTTCTATGGGAGACCCGACGGAATTTCGGGGGGTTTTGACGCATGAATTTGGACATATCGTGGACCTCGGGGTGCTTAACGGCATAAGCAAAACTAAAAACGGAAACTATACCGAGTTTGGTAAAGTAAAATTCGCCATTGACGATCCTTCATTGGAATACTATAGATATTCTTGGGACTCCGAGAACATCAGAAGCCAATCCGCCAACAAAAAAGACTTCTGTAGTGGCTACGGTATGACCAATCCTTTTGAAGATTTTGCCGAGTGCCACAACCTCTTCCTCAACAACCGCCAACTCTTTAGGACGATGGCAAGCGAAACTCCCGTTATGAAAAATAAATACAACTTCTTCGCCAATCTCTTTGATAATGCCATTCTCAAAGACAACGCTTACCAGCTCCCTTACAAAGAACGAAGACCTCGAGATACGACACTGATCTAG", "aa": "MQGDPLHSVFKTLIINGETGKRRGGATRKRITINLSSMGDPTEFRGVLTHEFGHIVDLGVLNGISKTKNGNYTEFGKVKFAIDDPSLEYYRYSWDSENIRSQSANKKDFCSGYGMTNPFEDFAECHNLFLNNRQLFRTMASETPVMKNKYNFFANLFDNAILKDNAYQLPYKERRPRDTTLI", "aa_hexdigest": "e304440beeb1ca1b412401ba9ec2171e", "start_type": "ATG", "rbs_motif": "TTCTT", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 798131, "stop": 798979, "observations": { "mutation": [ 798757 ], "stop": [ 798757 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A660MTJ4", "query_cov": 1.0, "subject_cov": 0.6232876712328768, "identity": 0.995, "score": 380.0, "evalue": 3.71e-132, "gene_start": 1, "gene_end": 182, "reference_start": 111, "reference_end": 292, "reference_sequence": "MDRKIFFSFTLFDLRKLIGASILFFLALVIGVKEAGEPSTMLQVDYFSEEIKPDHPSPSASPQNDPFSSLCQKNQLFCEKIIRTGTLTQSEKRSYANQYFQIANFLEENLMQGDPLHSVFKTLIINGETGKRRGGATRKRITINLSSMGDPTEFRGVLTHEFGHIVDLGVLNGISKTKNGNYTEFGKVKFAIDDPSLEYYRYSWDSENIRSQSANKKDFCSGYGMTNPFEDFAECHNLFLNNRQLFRTMASETPIMKNKYNFFANLFDNAILKDNAYQLPYKERRPRDTTLI" }, "paralog": false, "identity": 0.9929078014184397, "score": 571.0, "evalue": 7.14e-210, "description": "Internal stop codon at 798757. Nonsense mutation around 798757.", "psc": { "uniref90_id": "UniRef90_A0A660MTJ4", "uniref50_id": "UniRef50_A0A7W4E340", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A660MTJ4", "UniRef:UniRef50_A0A7W4E340" ] }, "pscc": { "uniref50_id": "UniRef50_A0A7W4E340", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7W4E340" ], "product": "Peptidase M43 pregnancy-associated plasma-A domain-containing protein" } }, "id": "NKOCPLAKPK_1507", "locus": "GCA003260355_01476" }, { "type": "cds", "sequence": "contig_1", "start": 798755, "stop": 799009, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGACAGGAAAATCTTCTTTAGCTTTACTCTCTTTGACCTTCGGAAACTCATCGGTGCTTCGATCTTGTTTTTTCTGGCTTTAGTCATTGGGGTAAAAGAAGCAGGCGAACCCTCTACTATGCTCCAGGTGGACTATTTCTCAGAAGAGATAAAGCCTGACCATCCAAGCCCATCAGCATCGCCACAAAACGATCCTTTCAGCTCCCTATGCCAAAAGAATCAGCTTTTCTGCGAGAAAATTATTCGAACTTGA", "aa": "MDRKIFFSFTLFDLRKLIGASILFFLALVIGVKEAGEPSTMLQVDYFSEEIKPDHPSPSASPQNDPFSSLCQKNQLFCEKIIRT", "aa_hexdigest": "7bfeb566eb9eac9b34129948590601ac", "start_type": "ATG", "rbs_motif": "TTTA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9543.968399999994, "isoelectric_point": 5.692845726013183 }, "id": "NKOCPLAKPK_1508", "locus": "GCA003260355_01477" }, { "type": "cds", "sequence": "contig_1", "start": 799122, "stop": 799712, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAGACTATTTTTGAGGGGATGATGATTGTAGAAAAGAAGATGTATAGTAAGTCAGGTTTCTACTTTGTTTATGCAGAAGAGGTAAAGTTTACCAATTGGGAGGAGTTTTTAGAAGAGTTTGTCGGGCACAGAATCTTTTTCCTCTCGCACACCGACGAACAGCTCCCAGCCCTCATTCCCGAAGCCTCACGTGCTCCAGCTTCCTCTGCTTCTTCCATTCCAGTCATAAAAGACAAAAATCCTGACTCAACATCCTCTGATTCTCCGCTTCCGACGGATTCAGAACCTCAAAGAAAGCCAACAACTCCAGCTCCATCAGCACCGAACGCTCAAAAACCTGCAAACATCCACCGCTTGAGTAAAACCCCTTCTGTCTTGCAGGCAATACTTGCCTTTGATCGTGAAAAGTCTCCTAACGGAACGATTTTCATCCTCTCTGTGGTTAAGGAGGAAAGTAAAGTACTCTTTGAAACGATGATTGATAGGGGATTAGATCAGAATTTTTTGCTCTTAGTAGAGAATATCACAGGAGGAAGCGGGAAAAACCTCTTCAAAGCCAAGCATCAGGGCACGAAAATCATCATTTGA", "aa": "MQTIFEGMMIVEKKMYSKSGFYFVYAEEVKFTNWEEFLEEFVGHRIFFLSHTDEQLPALIPEASRAPASSASSIPVIKDKNPDSTSSDSPLPTDSEPQRKPTTPAPSAPNAQKPANIHRLSKTPSVLQAILAFDREKSPNGTIFILSVVKEESKVLFETMIDRGLDQNFLLLVENITGGSGKNLFKAKHQGTKIII", "aa_hexdigest": "92d19153f1fdcaf8a7f4409cd3fe0559", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 21830.693999999992, "isoelectric_point": 6.1102144241333 }, "id": "NKOCPLAKPK_1509", "locus": "GCA003260355_01478" }, { "type": "cds", "sequence": "contig_1", "start": 799830, "stop": 800633, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAACCGCTCGTTTTGCCTCCTCTCAAGAAAATTACAGCTCCTCATACCCAAATCCTTACTGGAGAATACCCTTTTACTCGGACGGAAAACCTCAATCGCAAGAAATTTCAGGTCAAAAATTTGGAATTTAAAAAGCTTCTCTCCTCGCTCGCTACCAATAAACAGACCATTTTTGCCTTTTCCAACCGCTCCAAGCTTGATATCGCAAAAAACCTCCTCAATGAACTCGGAGTCAAGAACCTTGGATTCGTCAAAGAAGAACAAACCCTGAATCGTGAAGCCTTGCAAGCTTTCGTAAATAAAGGACAGTTTGAGGAGTCAGAATTCGCTTTTTTGCTCAAATACTTCTCTCATCTTGAGCAGGGATTAGGAGTTTTAGACCTTAATAGTAAGGGCGACTATGAAATCTACACTGCGGTCAAAGATCAGAGACTTACCGTCAATTACCCGATCATTTTAGCGACACATGGCGGGCTTTTTTCGCTTTTGGAGCAGCCGGAAAGGTATCAAAACTATGATATTGTCTTCTTTGACTGTGAGCGGTGGTATAAAAGCTATAATTTCTACCTGTCTCGCCCCTATGATCTCAATTATACGCTTAATTATCTTGATATGCTCAGCTATAAATACCACCTTGAGCAGGAAATCTCCCCAGGCCAAGAAGAAAAAGTTCAAAATCTGGCAACCGTCGAGGAGTTTAGACAGTTTTTTACGATTTTTATGGGGATTCTCGGGCAGGAAACGAAAGCCTTCTTTACCCATACCGAAGCCACAACCCAGACCCTAGACCCTTTGAAATGA", "aa": "MQPLVLPPLKKITAPHTQILTGEYPFTRTENLNRKKFQVKNLEFKKLLSSLATNKQTIFAFSNRSKLDIAKNLLNELGVKNLGFVKEEQTLNREALQAFVNKGQFEESEFAFLLKYFSHLEQGLGVLDLNSKGDYEIYTAVKDQRLTVNYPIILATHGGLFSLLEQPERYQNYDIVFFDCERWYKSYNFYLSRPYDLNYTLNYLDMLSYKYHLEQEISPGQEEKVQNLATVEEFRQFFTIFMGILGQETKAFFTHTEATTQTLDPLK", "aa_hexdigest": "894d458cebc642a2b26fe7b25cd0326d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 31243.25300000003, "isoelectric_point": 6.251288795471192 }, "id": "NKOCPLAKPK_1510", "locus": "GCA003260355_01479" }, { "type": "cds", "sequence": "contig_1", "start": 801027, "stop": 801293, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00929.31" ], "nt": "ATGCTCAGAAGTGATATTCGTATGATTGCCTTAGATTTTGAAACTACTGGGCTAGATACCGCGACCGATGAACCCATTCAGATTGGAATTGTGGAGTTTAATAACGAGGGAAAGATTATTGGCGGATTTCAATCGCTTATTCGTCCAGAGAAACCGCTCAAAGAATTAAAAAGTATTGTCAGCTTTATCACTGGTCTTTCAGTAGAAAAGCTAGAACATGCCCCTACGCGTGCTGAAGTAGAGCAGGAAATCTCAACCTTTTTCTGA", "aa": "MLRSDIRMIALDFETTGLDTATDEPIQIGIVEFNNEGKIIGGFQSLIRPEKPLKELKSIVSFITGLSVEKLEHAPTRAEVEQEISTFF", "aa_hexdigest": "b169a3fa0017cf4ceb3b4481ad8f5615", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00929.31", "name": "RNase_T", "length": 81, "aa_cov": 0.8977272727272727, "hmm_cov": 0.9876543209876543, "evalue": 3.619514544039294e-12, "score": 43.57316207885742, "start": 9, "stop": 87 } ], "seq_stats": { "molecular_weight": 9851.179799999993, "isoelectric_point": 4.660763359069825 }, "id": "NKOCPLAKPK_1511", "locus": "GCA003260355_01480" }, { "type": "cds", "sequence": "contig_1", "start": 801762, "stop": 801959, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTAGCTTTTGATTCTCAAGGAAATATATCCTTGGATCAAGGGCAATTATCAGAAAAACTACAAGCTTCCCAGCCACTTCACGCTCAGATTGAAGAGAATGTTGCTTTCGGAGCTAGGAAAGTATGGAAGAATTTAGTGAATTTTATGCAAGAAGGATTTGATACTAAAGAAGGAGAAAAAGGTGAGAACAATTGA", "aa": "MLAFDSQGNISLDQGQLSEKLQASQPLHAQIEENVAFGARKVWKNLVNFMQEGFDTKEGEKGENN", "aa_hexdigest": "02b2c15d107253bff3e55ce6040de521", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7266.958499999999, "isoelectric_point": 4.716010856628418 }, "id": "NKOCPLAKPK_1512", "locus": "GCA003260355_01481" }, { "type": "cds", "sequence": "contig_1", "start": 802173, "stop": 802334, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCGTTGCAGTGATTTTATTAGAGTTGTAGTTGATATTTACAGACATAAAGCTGGATATTCATTAGAGAGCACCAAAGGGCTCACTGATCAACAGCTTTTGTCGTTGAAAAATCCGGATGCTCTCCTTAGTAAAAAACTCAATACTGCGAAACACCTTTGA", "aa": "MRCSDFIRVVVDIYRHKAGYSLESTKGLTDQQLLSLKNPDALLSKKLNTAKHL", "aa_hexdigest": "b33879173285f587c8bc892c9dc4dd35", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6016.947399999999, "isoelectric_point": 9.579115486145017 }, "id": "NKOCPLAKPK_1513", "locus": "GCA003260355_01482" }, { "type": "cds", "sequence": "contig_1", "start": 802356, "stop": 802883, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00395.25" ], "nt": "ATGATCCGAGATCATACGATTACACCGCTTCAAGCACAGCTGATCCTTAACAACATCTTCACACTTTATCCTCATCTTGGTCAAAAAGAGAAGATAAAACTGATTGATACTACGAAATCAGTTTTAACCAAATCTGAAATGGCCGGATACCTTGTGGCAATTTTCCAATTGGAACCCCAAGAAACGGATAATCTATTTAGAGATATTCATAATCATCGCTATCAGGATGCAATTATTCAGCTTGCCCAGCTCGGGATAGTAGCTGGGAGAAACGGTAATTTCTACCCTGATAATGATACGCTCCGTGCAGACGGAGTAATTATGATTGCAAACAGTATGCTTGCGAAAGAACAAAAAGCTCTTGTTATCAAAAACTTTTATCATCTAAATAGTATTGCCGATGTCACCTATTTTGCAGCCTTTGCTCCCCATTTGGAATTTTTGCTTGATCATAAGATCGGAACTTCTTTACTTCGTCATGAACAATCAGGAGATTTGTTTCTCCCTTATGCTCTACTTACTAAGTGA", "aa": "MIRDHTITPLQAQLILNNIFTLYPHLGQKEKIKLIDTTKSVLTKSEMAGYLVAIFQLEPQETDNLFRDIHNHRYQDAIIQLAQLGIVAGRNGNFYPDNDTLRADGVIMIANSMLAKEQKALVIKNFYHLNSIADVTYFAAFAPHLEFLLDHKIGTSLLRHEQSGDLFLPYALLTK", "aa_hexdigest": "8f4b8735b25c60b8f0b3232f9c04b613", "start_type": "ATG", "rbs_motif": "TTCTT", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00395.25", "name": "SLH", "length": 42, "aa_cov": 0.22285714285714286, "hmm_cov": 0.9761904761904762, "evalue": 1.8105836283243335e-08, "score": 30.717483520507812, "start": 66, "stop": 104 } ], "seq_stats": { "molecular_weight": 19891.7293, "isoelectric_point": 6.4295358657836905 }, "id": "NKOCPLAKPK_1514", "locus": "GCA003260355_01483" }, { "type": "cds", "sequence": "contig_1", "start": 803224, "stop": 803526, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBFB52A", "UniRef:UniRef100_A0A328EHK0", "UniRef:UniRef50_A0A328EHK0", "UniRef:UniRef90_A0A328EHK0" ], "nt": "ATGAACACAACTAACACACCAAATCTTTTGGCTCAGGAACTAAGTGCAGAAGAACTTAAGCTTCAGAACGATGAAGAAACAAGAAACTACAAAAACAAAAAAGAAGAACTAAATATCAAAGACAGATTTGATGTATGGGACACACACTTGTATGGAGAACAGGTTAGTGCAACCTTGAATGCAACCAATAGTATCGCTTCAAGTGCTAGAGTGGCTAAGGCTAAAACTCAAGGAGACCTCCTTCTTGCTCTTCTCTCAAAAAAACCTCTGGATGCAGAGGAAGACTATGCTCAAGCTGCCTAA", "aa": "MNTTNTPNLLAQELSAEELKLQNDEETRNYKNKKEELNIKDRFDVWDTHLYGEQVSATLNATNSIASSARVAKAKTQGDLLLALLSKKPLDAEEDYAQAA", "aa_hexdigest": "e3c24caf16f172b01afb7f2fb12683c4", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000DBFB52A", "uniref100_id": "UniRef100_A0A328EHK0", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBFB52A", "UniRef:UniRef100_A0A328EHK0" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EHK0", "uniref90_id": "UniRef90_A0A328EHK0" }, "psc": { "uniref90_id": "UniRef90_A0A328EHK0", "uniref50_id": "UniRef50_A0A328EHK0" }, "pscc": { "uniref50_id": "UniRef50_A0A328EHK0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EHK0" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11167.238199999985, "isoelectric_point": 4.81803684234619 }, "id": "NKOCPLAKPK_1515", "locus": "GCA003260355_01484" }, { "type": "cds", "sequence": "contig_1", "start": 803553, "stop": 803792, "strand": "-", "frame": 2, "gene": "tsaE", "product": "tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex ATPase subunit type 1 TsaE", "db_xrefs": [ "GO:0002949", "SO:0001217", "UniRef:UniRef50_A0A351Y4W3", "UniRef:UniRef50_A0A660MW22", "UniRef:UniRef90_A0A328EH74" ], "nt": "GTGCAGAGTCCTACCTATACCTACATCAATATCTACGACAACAAGCTCTTACACATAGATCTTTATAGGCTTTCAAGTTTTTCCGAACTTGTAGAAAAAGGGATTTTAAATCAGATTGCTGAATTTGATTGGATTGCGATAGAGCGACCAAAGTGGGAAGACCAGCTTGATTTGCCTTCTCCTCTGGCTTTAGAGATCAGTAAAATCTCAGCCAGCCAAAGAGAAATAAAAAAACTCTAG", "aa": "MQSPTYTYINIYDNKLLHIDLYRLSSFSELVEKGILNQIAEFDWIAIERPKWEDQLDLPSPLALEISKISASQREIKKL", "aa_hexdigest": "c38152a642138b4d6fd84c51ba3b1f5f", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A351Y4W3", "query_cov": 0.9873417721518988, "subject_cov": 0.9873417721518988, "identity": 0.5770000000000001, "score": 97.8, "evalue": 3.28e-25, "valid": false, "uniref50_id": "UniRef50_A0A351Y4W3" }, "pscc": { "uniref50_id": "UniRef50_A0A351Y4W3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A351Y4W3" ] }, "genes": [ "tsaE" ], "truncated": "3-prime", "pseudogene": { "start": 803553, "stop": 803942, "observations": { "mutation": [ 803816 ], "stop": [ 803816 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EH74", "query_cov": 1.0, "subject_cov": 0.6124031007751938, "identity": 0.987, "score": 157.0, "evalue": 4.9e-48, "gene_start": 1, "gene_end": 79, "reference_start": 51, "reference_end": 129, "reference_sequence": "MLISSPQAMHDYGKSLVQETKILLLHGGLGAGKTTLIKGFAEGLGINPDQVQSPTYTYINIYDNKLLHIDLYRLSSFSELVEKGILNQIAEFDWIAIERPKWEDQLDLPSPLALEISKISASQREIKKL" }, "paralog": false, "identity": 0.9922480620155039, "score": 253.0, "evalue": 1.53e-88, "description": "Internal stop codon at 803816. Nonsense mutation around 803816.", "psc": { "uniref90_id": "UniRef90_A0A328EH74", "gene": "tsaE", "product": "tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex ATPase subunit type 1 TsaE", "uniref50_id": "UniRef50_A0A660MW22", "go_ids": [ "GO:0002949" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EH74", "UniRef:UniRef50_A0A660MW22", "GO:0002949" ] }, "pscc": { "uniref50_id": "UniRef50_A0A660MW22", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MW22" ], "product": "tRNA threonylcarbamoyladenosine biosynthesis protein TsaE" } }, "id": "NKOCPLAKPK_1516", "locus": "GCA003260355_01485" }, { "type": "cds", "sequence": "contig_1", "start": 803814, "stop": 803942, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02367.23" ], "nt": "ATGCTCATCTCTTCTCCACAAGCTATGCACGACTACGGAAAATCTCTTGTCCAAGAGACTAAAATCCTTTTGCTCCACGGCGGACTCGGAGCCGGGAAAACGACTCTTATCAAGGGTTTTGCTGAGTGA", "aa": "MLISSPQAMHDYGKSLVQETKILLLHGGLGAGKTTLIKGFAE", "aa_hexdigest": "ce92f41175514b244165f0c70318f693", "start_type": "ATG", "rbs_motif": "TTTA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02367.23", "name": "TsaE", "length": 42, "aa_cov": 0.9285714285714286, "hmm_cov": 1.0, "evalue": 3.0637262001528776e-09, "score": 33.34989547729492, "start": 4, "stop": 42 } ], "seq_stats": { "molecular_weight": 4455.203199999999, "isoelectric_point": 8.182343482971191 }, "id": "NKOCPLAKPK_1517", "locus": "GCA003260355_01486" }, { "type": "cds", "sequence": "contig_1", "start": 804090, "stop": 804296, "strand": "+", "frame": 3, "gene": null, "product": "tRNA-binding domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A948C653" ], "nt": "ATGAAATATCACTTACAAATCCTGAGGAAATATCTCTCTCTAGATACAACGCCTCAAGATATCGCAAAAAATTTGATTCTCAAGACTTGTGAAATCGAGGAAGTAATAGAAAGGCAGATCCCTCGCTCTATTGTGATCGGGAAAATAGAACAGGTCGAGAAACACCCTGATGCTGATAAACTCAATATTTGCCAGGTGAACTGCTGA", "aa": "MKYHLQILRKYLSLDTTPQDIAKNLILKTCEIEEVIERQIPRSIVIGKIEQVEKHPDADKLNICQVNC", "aa_hexdigest": "44feddeff45580af3e2dcd591ed65111", "start_type": "ATG", "rbs_motif": "TTTTTA", "psc": { "uniref90_id": "UniRef90_A0A948C653", "query_cov": 1.0, "subject_cov": 0.9577464788732394, "identity": 0.618, "score": 94.4, "evalue": 3.97e-24, "valid": false, "product": "tRNA-binding domain-containing protein", "uniref50_id": "UniRef50_A0A948C653" }, "pscc": { "uniref50_id": "UniRef50_A0A948C653", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A948C653" ], "product": "tRNA-binding domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_1518", "locus": "GCA003260355_01487" }, { "type": "cds", "sequence": "contig_1", "start": 804336, "stop": 805112, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03483.22" ], "nt": "GTGGCTGAAGGGCTTTTTGTAGCGACTGCTCTGGTAGGCACGCTATTTCCTGAGGCAGGGATTACGATTGCAAAAAGGGCAATGAGAGGTGTAGACTCTGAAGGGATGATTTGCTCAAAAGGAGAGCTTGGTATTAATGAAGATAAGGATACCCACTGGATTTGGGACCTTGCACAAGACTTGGAGGTATCTGATACGGATCTTGGTCGTCCTCTCAGCGAAGTATTCCCTCGATTGGAAGCTACGATCTTGGAAGTAGACAGTAAAAGCTTGACGAATAGGCCAGATCTCACGGGACACTTTGGAGTTGCGGTCGAGTTGAATGCTATCTATCCAGATGCTTTGAAGAAGTTTAACGGCATCAAAAAGTGGATTGAGAGTTTTCGTGATACCAACATCTTGGAGGTGCTGGAACATACAGAAAAACCGCTTAACCGTCAAGTTCGTGGGCTCTCTAAGGCGGTCAATACCTATATTGCCCTGGGAATCAAGAATATCGAGGTCAAAAAATCAGATTTCTTTTCTCGTTTACAGGTTTTGGACCTTGGGAGTAATCCTATCAACAATTGGGTAGATTTTTCGAATCTTTTTATGAATACGGTTGGTTTGCCAATTCATTGCTTTGATGCAGATAAGATTGAAGGTGCGATTATTATTAGAGACGCAAACGAAGGAGAGCTTTTTACGGATCTTTTTGGCACGGAGCATCGCTTGCAGGCGACAGACCTTGTGATCGCTGATGAAAAGAAAGTGCTTGCACTCGCTGGAGTGATTTGA", "aa": "MAEGLFVATALVGTLFPEAGITIAKRAMRGVDSEGMICSKGELGINEDKDTHWIWDLAQDLEVSDTDLGRPLSEVFPRLEATILEVDSKSLTNRPDLTGHFGVAVELNAIYPDALKKFNGIKKWIESFRDTNILEVLEHTEKPLNRQVRGLSKAVNTYIALGIKNIEVKKSDFFSRLQVLDLGSNPINNWVDFSNLFMNTVGLPIHCFDADKIEGAIIIRDANEGELFTDLFGTEHRLQATDLVIADEKKVLALAGVI", "aa_hexdigest": "92fede7645cef0c41ffa1f8f998f00b6", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03483.22", "name": "B3_4", "length": 104, "aa_cov": 0.3992248062015504, "hmm_cov": 1.0, "evalue": 5.345112785868192e-25, "score": 84.44542694091797, "start": 156, "stop": 258 } ], "seq_stats": { "molecular_weight": 28581.343700000027, "isoelectric_point": 4.858108329772949 }, "id": "NKOCPLAKPK_1519", "locus": "GCA003260355_01488" }, { "type": "cds", "sequence": "contig_1", "start": 805491, "stop": 805754, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03484.21" ], "nt": "GTGGCAGGCGATGTCGTGAGCGTTCCCCTTTGGAGAGGACCAGATGATATCAACATCAAGGAAGATGTTGCTGAAGAAATTGCTAGGATCTGGGGCTATGACCAAATAAGTCCCCAAGCTTTGCTTTCAGAAGTGAAGGCTCAGCCGTTTAGTGAGAGTGTCGCTATTCTCAGAGCGGTAGAACAAGCGATGGTAGAAATTTTTGATTTTGATCAGGTAGAAACCTATCCTCGGGTGGCAAAAACTCAAGTGGAACTCTTGTGA", "aa": "MAGDVVSVPLWRGPDDINIKEDVAEEIARIWGYDQISPQALLSEVKAQPFSESVAILRAVEQAMVEIFDFDQVETYPRVAKTQVELL", "aa_hexdigest": "58f52c3f56dee74ca76c617ee1f88ce1", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03484.21", "name": "B5", "length": 28, "aa_cov": 0.3218390804597701, "hmm_cov": 0.9285714285714286, "evalue": 1.0593969653681638e-08, "score": 31.695316314697266, "start": 6, "stop": 33 } ], "seq_stats": { "molecular_weight": 9775.985299999995, "isoelectric_point": 4.182633781433105 }, "id": "NKOCPLAKPK_1520", "locus": "GCA003260355_01489" }, { "type": "cds", "sequence": "contig_1", "start": 805824, "stop": 806195, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF17759.6" ], "nt": "ATGCTTTATAATCTGCTCCCTATTGCTAGCAAAAGTAGTAAATTCTTTGATCATTTTAAGATTTTTGATCTAGGGAAAGTATGGAAGCCTGGAATTTCTGAGCAAGCACTCCATTTAGACCAGAGATATGCCGAAGAACACTTCTGCGAGGAGCTTACTCTCGGAGCATGCCGATACGAAAAGTCCTGTGCAAGCCGATCTGACGATCCGCTCCTCAAGATGAAAGCTTTGCTTACAGCTCTTTTTAAGAGGCTGGAGATCAAAGGCAAACTCTTCTTTGAACCATCAGCTTTCAGCTGTTTTCATCCGAAAAAACAGGCAAAACTCTTGCTTAGAAATGGTGAAAAATTAGTAGAAATAGGACAAATCTGA", "aa": "MLYNLLPIASKSSKFFDHFKIFDLGKVWKPGISEQALHLDQRYAEEHFCEELTLGACRYEKSCASRSDDPLLKMKALLTALFKRLEIKGKLFFEPSAFSCFHPKKQAKLLLRNGEKLVEIGQI", "aa_hexdigest": "8b2341d5062b6ccf12be463dad446cec", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF17759.6", "name": "tRNA_synthFbeta", "length": 124, "aa_cov": 0.959349593495935, "hmm_cov": 0.8951612903225806, "evalue": 1.2682400790545286e-07, "score": 27.782482147216797, "start": 6, "stop": 123 } ], "seq_stats": { "molecular_weight": 14180.505999999976, "isoelectric_point": 9.062401390075681 }, "id": "NKOCPLAKPK_1521", "locus": "GCA003260355_01490" }, { "type": "cds", "sequence": "contig_1", "start": 806340, "stop": 806600, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03147.19" ], "nt": "GTGTGGAGAGACCTTTCTTTTGTAGTGGATGCGAAGACTTCTTTTGAAAAGGTAATTACGACGTTAGAGAAGATGCCCCAAATTAGTAAAGTCAGACTTTTTGATCTCTATCAAGGAGAGAACCTCGGAACCGATAAGAAATCCCTTGCGGTGCAGATCAAAATTAAGGGTGATGGTGCGATGACTACGGAAGCCATCAATGCCGTAATGCAAGATGCAATCAAAAAGGTGGAAGCAACAGGAGCTCAGCTAAGAGCGTAA", "aa": "MWRDLSFVVDAKTSFEKVITTLEKMPQISKVRLFDLYQGENLGTDKKSLAVQIKIKGDGAMTTEAINAVMQDAIKKVEATGAQLRA", "aa_hexdigest": "919eeb58d906577ed1041cb8f49abad3", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03147.19", "name": "FDX-ACB", "length": 88, "aa_cov": 0.9767441860465116, "hmm_cov": 1.0, "evalue": 8.401665316392518e-18, "score": 61.113059997558594, "start": 2, "stop": 85 } ], "seq_stats": { "molecular_weight": 9532.004599999997, "isoelectric_point": 9.003348350524902 }, "id": "NKOCPLAKPK_1522", "locus": "GCA003260355_01491" }, { "type": "cds", "sequence": "contig_1", "start": 806779, "stop": 807060, "strand": "+", "frame": 1, "gene": null, "product": "Disulfide bond formation protein B", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EI03", "UniRef:UniRef90_A0A328EI03" ], "nt": "ATGTATCCGCTGGTTGCAATTTCAGTATATGGACTGATCAGAAAAGATAAGGGAATCTGTTTTTTGACAGGGGCTTTGTCGCTTGTTGGTGTGCTTTTGGCAGGATACCACTATTTGATTCAATATCAGGCATCGCTTAATGTATTTACCTGTCAGGCTGGGAATGATTGCACACATATGGGATGGCATATTGGATTTGTAACAATTCCGTTTCTTGAGCTCGTGGCTTTTGCGGTGATTCTGACCTGTAGCATCCTGATGCTTGTCAGGAGAAAGAAATAA", "aa": "MYPLVAISVYGLIRKDKGICFLTGALSLVGVLLAGYHYLIQYQASLNVFTCQAGNDCTHMGWHIGFVTIPFLELVAFAVILTCSILMLVRRKK", "aa_hexdigest": "b5ac4c5437cacfd5bd6e06472afd608f", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 806623, "stop": 807060, "observations": { "mutation": [ 806704 ], "stop": [ 806704 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EI03", "query_cov": 1.0, "subject_cov": 0.6413793103448275, "identity": 0.9890000000000001, "score": 186.0, "evalue": 2.7e-59, "gene_start": 1, "gene_end": 93, "reference_start": 53, "reference_end": 145, "reference_sequence": "MSYRNLLVIILIQASLAMLGSLYFSNFGDPLNGLFSGTGFEPCHLCWRGRILMYPLVAISVYGLIRKDKGICFLTGALSLVGVLLAGYHYLIQYQASLNVFSCQAGNDCTHMGWHIGFVTIPFLELVAFAVILTCSILMLVRRKK" }, "paralog": false, "identity": 0.9793103448275862, "score": 292.0, "evalue": 2.77e-103, "description": "Internal stop codon at 806704. Nonsense mutation around 806704.", "psc": { "uniref90_id": "UniRef90_A0A328EI03", "product": "Disulfide bond formation protein B", "uniref50_id": "UniRef50_A0A328EI03", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EI03", "UniRef:UniRef50_A0A328EI03" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EI03", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EI03" ], "product": "Disulfide bond formation protein B" } }, "id": "NKOCPLAKPK_1523", "locus": "GCA003260355_01492" }, { "type": "cds", "sequence": "contig_1", "start": 807652, "stop": 807753, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCACACTTCACTCAGATGGGACTCGGCTTCGAGTTTGATCCTGTCAAAAAATATGTTTATACCGTGATCCATTATGGGACAGAGATCGAGGGCGCTTAA", "aa": "MPHFTQMGLGFEFDPVKKYVYTVIHYGTEIEGA", "aa_hexdigest": "e3d47bd30b889a363832f7644c09af4d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3806.321100000002, "isoelectric_point": 5.348060417175293 }, "id": "NKOCPLAKPK_1524", "locus": "GCA003260355_01493" }, { "type": "cds", "sequence": "contig_1", "start": 807784, "stop": 808155, "strand": "-", "frame": 1, "gene": null, "product": "SCP domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EKN1", "UniRef:UniRef90_A0A328EKN1" ], "nt": "TTGCCAAACGTTGACTTTGAAAAAGTGAGAACGGCACGACTTGGACGACATAACGACCTGAGAGCCAAGAAAAACCTGAAACCTTACCGCTATCAGAGTGAATTGGAGAGAACCGCTAAAAAGCGAGCGGATTATCTCGTTTCCCTCGGGAGACGAACGCACCAAAGGTCCGCCAAAGACGGATATTATAGTTATCCCGCTATTAAGGTTCGATTTTCAGATCAGGGTGTAAAATTTACTGGAGAAACAGCTGGAAAATCTTCTTTTTCAGAAAGTATCGGCTATAGAACCTATCGCTGTTCTAGTCAAGATTGCACCCAGGAGCTTATCGACGCTACCAAGAAAATCTTTGACGCTTTCGCCGCCGAGTGA", "aa": "MPNVDFEKVRTARLGRHNDLRAKKNLKPYRYQSELERTAKKRADYLVSLGRRTHQRSAKDGYYSYPAIKVRFSDQGVKFTGETAGKSSFSESIGYRTYRCSSQDCTQELIDATKKIFDAFAAE", "aa_hexdigest": "f9367bf993252475f966311a5d299d79", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 807655, "stop": 808455, "observations": { "mutation": [ 807777, 807786, 808203 ], "stop": [ 807777, 807786, 808203 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EKN1", "query_cov": 1.0, "subject_cov": 0.43309859154929575, "identity": 0.9590000000000001, "score": 238.0, "evalue": 3.32e-77, "gene_start": 1, "gene_end": 123, "reference_start": 118, "reference_end": 240, "reference_sequence": "MENVSTLTKNCTKKIGFLLLVASLLLTNISALAYSDATNQLITSAEQKMKTMTAQEQSTYLQTLKNTLTLPALQSNAPAEVQQIAQELDQRIKERVVEATSGFPPTQTPTSQQSYQTLPNVDFERVRTARLGRHNDLRAKKNLKPYRYHSELERTAKKRADYLVSLGRRTHQRSAKDGYYSYPAIKVRFSDQGVKFTGETAGKSAFSESIGYRTYRCSSQDCTQELIDATKKIFDGFAAEGEGGPHYRALMMPHFTQMGLGFEFDPVKKYVYTVIHYGTEIEGT" }, "paralog": false, "identity": 0.9473684210526315, "score": 499.0, "evalue": 2.27e-182, "description": "Internal stop codon at 807777, 807786, 808203. Nonsense mutation around 807777, 807786, 808203.", "psc": { "uniref90_id": "UniRef90_A0A328EKN1", "product": "SCP domain-containing protein", "uniref50_id": "UniRef50_A0A328EKN1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EKN1", "UniRef:UniRef50_A0A328EKN1" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EKN1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EKN1" ], "product": "SCP domain-containing protein" } }, "id": "NKOCPLAKPK_1525", "locus": "GCA003260355_01494" }, { "type": "cds", "sequence": "contig_1", "start": 808201, "stop": 808362, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAACCATGACCGTTCAAGAGCAGTCAACCTACTTACAAACGCTCAAAAATACGCTTACACTGCCAACACTCCAGAGCAATGCTCCTGCTGAGGTGCAACAAATCGCTCAAGAGCTGGACCAACGGATCAAAGAGAGAGTAGTAGAGGCAACTGCTTGA", "aa": "MKTMTVQEQSTYLQTLKNTLTLPTLQSNAPAEVQQIAQELDQRIKERVVEATA", "aa_hexdigest": "30a19207c4dc445cbfa2be5b82b20584", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5988.755500000002, "isoelectric_point": 5.1057558059692365 }, "id": "NKOCPLAKPK_1526", "locus": "GCA003260355_01495" }, { "type": "cds", "sequence": "contig_1", "start": 808728, "stop": 809024, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAAATTCGGAGAAAAACTTTTCGATGCTTTTGGACCACTGATTTTCCTCGGCTTCGGTGGAATGCTTTCTCTTGCATTGGCAATCTTTAACATTCTCCCAATCCCAGCCGTAGATGGAGGAAGATTCTGGGCAGTAATCTTGCAAAAACTCTTCCGCATCAGTGACGAAAAGTTCTCGACAGTTGAAGGACGAATAAACTTTGTCTTTTTTCGAGCACTGATGCTGTTGGGGGTAGTGATTATCCTCAAAGACCTCGTTTTTCGACGGGGTATCAAAATCCCATTCATTGGATAG", "aa": "MKFGEKLFDAFGPLIFLGFGGMLSLALAIFNILPIPAVDGGRFWAVILQKLFRISDEKFSTVEGRINFVFFRALMLLGVVIILKDLVFRRGIKIPFIG", "aa_hexdigest": "f826da85857f17593042fc910adab937", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11009.263299999988, "isoelectric_point": 10.266219520568846 }, "id": "NKOCPLAKPK_1527", "locus": "GCA003260355_01496" }, { "type": "cds", "sequence": "contig_1", "start": 809178, "stop": 809822, "strand": "-", "frame": 2, "gene": "rseP", "product": "RIP metalloprotease RseP", "db_xrefs": [ "GO:0004222", "GO:0006508", "GO:0016020", "SO:0001217", "UniRef:UniRef50_A0A328EHG9", "UniRef:UniRef90_A0A328EHG9" ], "nt": "ATGCCACCCAGAATCGCCACACTACGAACCGATAAATCAGGCACAAAATATACATTAAATCTGATCCCACTCGGAGGATTTGTTGCCCTCAAGGGAGAAGACGGCACAAATTCCGCAGAAAATACCGATGCCAATAGCTTCGTAAAGGCAAAACTCCGAAAGAAACTCATCATTATTTCAGCGGGAGTGATGATGAACTTTTTGATTGCTCGAGTAGTCTTTTCTTCGCTCTTTTTCATTGGGACGAAACCCATCAGTGTCTTGCCAGATGATTTAGAAGGGATTTCTTCCCAAAGTTACCTGATGCCGAGCATAAGCTTCCTGAAATCTGAGGGATTTTTGAGTGGTGAGATCAAAGACACTGGGGTAGTTGTGGAACAAGTGCTCTCTGGTAGTATCGCAGCCTCACTTGGTATCAAAAGTGGTGCAGTCCTCGTGGATATTAATGGTCAACCAATCTCAACCCTTACAATCAATAGAATCCTCTCCAAGCTCAGCAATACCAATGAGAATACCATCCATTTTGCAGAGGCGCTTGAGATGTCTTCTCTCCAAGAGGGGAAATTCTCTTGTGAGTCTGATTGTAAACTTGGGATCGTCTTCCACCAAGATGGTGATATTGAAGTTCTCCCTGTAAAATTCTGA", "aa": "MPPRIATLRTDKSGTKYTLNLIPLGGFVALKGEDGTNSAENTDANSFVKAKLRKKLIIISAGVMMNFLIARVVFSSLFFIGTKPISVLPDDLEGISSQSYLMPSISFLKSEGFLSGEIKDTGVVVEQVLSGSIAASLGIKSGAVLVDINGQPISTLTINRILSKLSNTNENTIHFAEALEMSSLQEGKFSCESDCKLGIVFHQDGDIEVLPVKF", "aa_hexdigest": "0ea8911d972ac9abe57e943d9b6263e2", "start_type": "ATG", "rbs_motif": null, "genes": [ "rseP" ], "truncated": "both", "pseudogene": { "start": 808875, "stop": 809939, "observations": { "mutation": [ 809039, 809108, 809180, 809831, 809852, 809921, 809933 ], "stop": [ 809039, 809108, 809180, 809831, 809852, 809921, 809933 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EHG9", "query_cov": 1.0, "subject_cov": 0.5310173697270472, "identity": 0.981, "score": 399.0, "evalue": 3.09e-137, "gene_start": 1, "gene_end": 214, "reference_start": 40, "reference_end": 253, "reference_sequence": "MLGIILGILTFVLLVILHELGHFIAAKKSGVQVKEFGLGMPPRIATLRTDKSGTKYTLNLIPLGGFVALKGEDGTNSAENTDANSFVKAKLRKKLIIISAGVMMNFLIARVVFSSLFFIGTKPISVLPDDLEGISSQSYLMPSISFLKSEGFLSGEIKDTGVVVEQVLSGSIAASLGIKSGAVLVDINGQPISTLTINRILSKLTNTKENTIHFAEALEMSSLQEGKFSCESDCKLGIVFRQEGDIEVLPVKFGLGKAMLAGFKEIKAERDLTMTSLGRIGSKLFSFNANDTKSALNQLTGPVGAVKFGEKLFDAFGPLIFLGFGGMLSLALAIFNILPIPAVDGGRFWAVILQKLFRISDEKFSTVEGRINFVFFRALMLLGVVIILKDLVFRRGIKIPFIG" }, "paralog": false, "identity": 0.9689265536723164, "score": 646.0, "evalue": 4.37e-237, "description": "Internal stop codon at 809039, 809108, 809180, 809831, 809852, 809921, 809933. Nonsense mutation around 809039, 809108, 809180, 809831, 809852, 809921, 809933.", "psc": { "uniref90_id": "UniRef90_A0A328EHG9", "gene": "rseP", "product": "RIP metalloprotease RseP", "uniref50_id": "UniRef50_A0A328EHG9", "go_ids": [ "GO:0004222", "GO:0006508", "GO:0016020" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EHG9", "UniRef:UniRef50_A0A328EHG9", "GO:0004222", "GO:0006508", "GO:0016020" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EHG9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EHG9" ], "product": "PDZ domain-containing protein" } }, "id": "NKOCPLAKPK_1528", "locus": "GCA003260355_01497" }, { "type": "cds", "sequence": "contig_1", "start": 810262, "stop": 810633, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGGAAAGACTGGGAATGCTACAGGTCCTCACGTTCATTTTCAGATAGATCACAATAACGGAGAACATCCCTACTTTCCCAAAAATTGTAGCGGGACGATTGCAGAAGTGGTGAATGAGGCGAAGTGCCGAAACCAAGTAAAAGCTAATACCTTAGATCCCATCCTCTTTTTGGAGACGCAAGGAGAAATCTTTCTCGCAGAACAACAGCCAAAAAAATCTGAATCTCTCATCAATTCAATGCTTCCCCAAGAATTAAACTATACCCTCCAGTCTCCAATCGCTTTGCTAGGAAGTGGTATAACACTTGAAATCACCCCTCCTATTGCAAAAGGAGAAGTTTTCCTCAAGGATGACATTACAATTACTTGA", "aa": "MGKTGNATGPHVHFQIDHNNGEHPYFPKNCSGTIAEVVNEAKCRNQVKANTLDPILFLETQGEIFLAEQQPKKSESLINSMLPQELNYTLQSPIALLGSGITLEITPPIAKGEVFLKDDITIT", "aa_hexdigest": "255c1864881d96e0df961b6cf484e5ee", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13471.173599999986, "isoelectric_point": 5.245409202575683 }, "id": "NKOCPLAKPK_1529", "locus": "GCA003260355_01498" }, { "type": "cds", "sequence": "contig_1", "start": 810667, "stop": 810840, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01551.29" ], "nt": "GTGGATATTGCAAGCACTGAGGGTACGCCTGTTAGTGCGGCGGCAGATGGAGAAGTGGTCTATGCCCAGCAAAAAGGAGAACGAGGAAAGGTCATTGTCATCAAACATGATCGAAAAGGTACGACCCTTCATACCGTGTATGCCCACTTAGAAAGCATTGCTGTAAAGGTTTGA", "aa": "MDIASTEGTPVSAAADGEVVYAQQKGERGKVIVIKHDRKGTTLHTVYAHLESIAVKV", "aa_hexdigest": "8dc2cfd21beb8602392dfe333cec8d94", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01551.29", "name": "Peptidase_M23", "length": 56, "aa_cov": 0.9824561403508771, "hmm_cov": 0.9464285714285714, "evalue": 1.9440831928903107e-15, "score": 53.294517517089844, "start": 1, "stop": 56 } ], "seq_stats": { "molecular_weight": 6076.847100000001, "isoelectric_point": 6.819280815124511 }, "id": "NKOCPLAKPK_1530", "locus": "GCA003260355_01499" }, { "type": "cds", "sequence": "contig_1", "start": 810985, "stop": 811095, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAGCTATTAATAGCCTTACTTATACTACTCACGCTTCCTGTGGTCTATGGATGGAGCTATCCACTCAAAGAAATAGAAGGCTCCTTTTGCCCTGAAAAAGAATGA", "aa": "MKKLLIALLILLTLPVVYGWSYPLKEIEGSFCPEKE", "aa_hexdigest": "d6d9713efd342ddc98842205b825b9a4", "start_type": "ATG", "rbs_motif": "TTTA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4136.0097000000005, "isoelectric_point": 6.131642723083496 }, "id": "NKOCPLAKPK_1531", "locus": "GCA003260355_01500" }, { "type": "cds", "sequence": "contig_1", "start": 811467, "stop": 811652, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCTCAAAAAATCACTTCAAACACTTCTCCTAAAAATTACCAAGTATCGTACAGGAATTGATCAAAGGGTAACTTATTTGAGGAATAATCAATCACTCATCTTGAGGCATTATGATATTCTAAAACCAACCCTCCTTTCTGAATTGTATAGAATTGCATCGACATTAAGAATCCATCAGGAATAA", "aa": "MLKKSLQTLLLKITKYRTGIDQRVTYLRNNQSLILRHYDILKPTLLSELYRIASTLRIHQE", "aa_hexdigest": "c72cfcdd72ed06854002054a972c9b5f", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7312.5855999999985, "isoelectric_point": 10.277179145812983 }, "id": "NKOCPLAKPK_1532", "locus": "GCA003260355_01501" }, { "type": "cds", "sequence": "contig_1", "start": 812155, "stop": 812316, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00482.28" ], "nt": "ATGTCTAATTTCTACCGTAATACATTCAAGACTTCAATAGATGTTGCTATGTCAATGATTGAGCCAATTTTGATGATGTTTGTGGCAGCCATTGTGGGAACTATCGTAGCTTCAATCTTCCTGCCAATGGGAAGTATGATGGAAGTTGTCCAGAGGCTATAG", "aa": "MSNFYRNTFKTSIDVAMSMIEPILMMFVAAIVGTIVASIFLPMGSMMEVVQRL", "aa_hexdigest": "54c308a2c243c504719dd301b823dacc", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00482.28", "name": "T2SSF", "length": 42, "aa_cov": 0.7924528301886793, "hmm_cov": 1.0, "evalue": 2.791427929731353e-07, "score": 27.02073097229004, "start": 1, "stop": 42 } ], "seq_stats": { "molecular_weight": 5934.192399999998, "isoelectric_point": 5.937594413757325 }, "id": "NKOCPLAKPK_1533", "locus": "GCA003260355_01502" }, { "type": "cds", "sequence": "contig_1", "start": 812341, "stop": 812673, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00482.28" ], "nt": "GTGGGATACAAATTGCTTTACGCAAAACTTCCTCTATTTAAGATTTTTATAGATAAGCTTTTTATCACGACACCAGTTATTAAAGATGTTGTAAAAACATTCTATATGTACAGGTTTTCGAGCTTGTTGTCCCAATTTTACGAAGCTGGAGTAAGTCCTGTAACTTCACTTAATCTTCTAGGAGGAATTTTTGATAATTTTCATTACAAAAAAAAGATGATGGATGTGAGATCAGATTTAGAGGCTGGATTTACACTCTACGAGTCACTAGCTTCATCTACCTTGTTCGATCCTATCCTTGTCCAAATTATTAATGTTGGAGAAAATACCTGA", "aa": "MGYKLLYAKLPLFKIFIDKLFITTPVIKDVVKTFYMYRFSSLLSQFYEAGVSPVTSLNLLGGIFDNFHYKKKMMDVRSDLEAGFTLYESLASSTLFDPILVQIINVGENT", "aa_hexdigest": "c0845e1fad609fd3e448a482a9a5b7d8", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00482.28", "name": "T2SSF", "length": 73, "aa_cov": 0.6454545454545455, "hmm_cov": 1.0, "evalue": 9.486693760805831e-09, "score": 31.76603126525879, "start": 39, "stop": 109 } ], "seq_stats": { "molecular_weight": 12581.636299999987, "isoelectric_point": 7.899585151672363 }, "id": "NKOCPLAKPK_1534", "locus": "GCA003260355_01503" }, { "type": "cds", "sequence": "contig_1", "start": 812827, "stop": 813210, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00482.28" ], "nt": "TTGATTCAGCAGCTCAATAAGCCAAGCCTGAAGCAAAAGGTAAATTTCTTTAGACTTTTGTCCGTATCCCAAAATGCTGGATTGGGAATTAGAGAATCTCTTATCTCTATCAAAAAATCAGAAACTCATAAAGGATTCAGGGAAATTCTTGAAGACCTGATCGTATCTCTTACCGAAGGTGCATCTCTCGCTCAAGCATTACAAGACCATAATTACTTTTTTCATTCAGATGAAATTGAACTAGTACAGTCCGCACAAGTAACTGGAAATATGACGAGTGTTCTGGGGCAAATTGCGGATGAGTTAGAAAATACTCAAGAAATTAATCAAAAAATCTCAAAAGCTATGACTTATCCTACTATGGTGCTCCTCATGGCGATCTGA", "aa": "MIQQLNKPSLKQKVNFFRLLSVSQNAGLGIRESLISIKKSETHKGFREILEDLIVSLTEGASLAQALQDHNYFFHSDEIELVQSAQVTGNMTSVLGQIADELENTQEINQKISKAMTYPTMVLLMAI", "aa_hexdigest": "74475b7142123b5f0620c2eebfe439d3", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00482.28", "name": "T2SSF", "length": 113, "aa_cov": 0.8818897637795275, "hmm_cov": 1.0, "evalue": 1.0639477494601593e-22, "score": 76.83809661865234, "start": 16, "stop": 127 } ], "seq_stats": { "molecular_weight": 14208.181599999987, "isoelectric_point": 5.556375312805175 }, "id": "NKOCPLAKPK_1535", "locus": "GCA003260355_01504" }, { "type": "cds", "sequence": "contig_1", "start": 813280, "stop": 813462, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGACTACACAGACGATATAAAAATGCTGATCTTGGATGGAAAATCAGCTTTTGATATTGGGCATTATGCTCTGGCAAAAGGAATGATTGATCTTGAAAGAGATGGAATTTTTAAGATTATCCACGGAAATCTCTCTTTAGATGAGCTATATAGATACGTAAGGATGAGGAATAAAAGATAA", "aa": "MDYTDDIKMLILDGKSAFDIGHYALAKGMIDLERDGIFKIIHGNLSLDELYRYVRMRNKR", "aa_hexdigest": "ba3e4fc51a9166fb585e93f212188cd3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7050.126799999998, "isoelectric_point": 6.7095247268676745 }, "id": "NKOCPLAKPK_1536", "locus": "GCA003260355_01505" }, { "type": "cds", "sequence": "contig_1", "start": 813601, "stop": 813879, "strand": "-", "frame": 1, "gene": null, "product": "Bacterial type II secretion system protein E domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3D0UTB2" ], "nt": "ATGGCGATGGAGTCTTCAATGACGGGACATCTTGTTTTTTCAACAATTCATACGAATTCAGCAGTAGAAACCCTAACAAGAGCATTTAATCTTGGAGCAAAGCCTTTTATGGTTGCTGGAACGTTTAATCTCGTGATTGCACAGAGACTTTGTAGAAAAATTTGTCCACATTGTGCAACTAAGATCAACGTTCAGACTGATCCTAAGTATCTCAATGCTCTAGAAACATTCAAAGAGTTTGATAAAGATGCTTTGAAAGATGAATTAATAAGCAGATGA", "aa": "MAMESSMTGHLVFSTIHTNSAVETLTRAFNLGAKPFMVAGTFNLVIAQRLCRKICPHCATKINVQTDPKYLNALETFKEFDKDALKDELISR", "aa_hexdigest": "bb1294344fb697a73b119080d8af28d0", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3D0UTB2", "query_cov": 0.8913043478260869, "subject_cov": 0.8631578947368421, "identity": 0.573, "score": 104.0, "evalue": 1.57e-27, "valid": false, "product": "Bacterial type II secretion system protein E domain-containing protein", "uniref50_id": "UniRef50_A0A3D0UTB2" }, "pscc": { "uniref50_id": "UniRef50_A0A3D0UTB2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3D0UTB2" ], "product": "Bacterial type II secretion system protein E domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_1537", "locus": "GCA003260355_01506" }, { "type": "cds", "sequence": "contig_1", "start": 813907, "stop": 814356, "strand": "-", "frame": 1, "gene": null, "product": "GspE/PulE family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00166D045C" ], "nt": "ATGAGACCGGATGAGAAGAGGCTTCCTCAGGATGCCAGAGTTTCCAGTATTACCCAGACAAATAAAGAAATAGATCTTCGTGCGAATACACTACCAACCGTTTGGGGAGAAAAACTTGTAATGAGAATTGTGGATAAATCAAAATCAATTCCAGCGCTCAATCAATTGGGAATCGTTGGATCAAATAAGGTCACAATTGAAAAGCATCTTTTGGATCCGAATGGGATTTTGCTTAATACAGGACCAACAGGATCTGGAAAGACAACAACACTTTATGCAGCATTAAATGAAATTAATACTATAGAGAGGAATATCATTACTTATGAAGACCCAGTTGAAAATAAAATGGAAGGACTCAACCAATCTCAGGTAAGATCAGATATTGGATATACGTTCCTCTCAGGACTAAGATCAGCATTGAGACAGGATCCTGATGTAATCATGGTGTGA", "aa": "MRPDEKRLPQDARVSSITQTNKEIDLRANTLPTVWGEKLVMRIVDKSKSIPALNQLGIVGSNKVTIEKHLLDPNGILLNTGPTGSGKTTTLYAALNEINTIERNIITYEDPVENKMEGLNQSQVRSDIGYTFLSGLRSALRQDPDVIMV", "aa_hexdigest": "b34a5784d512be1688381cd805245598", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00166D045C", "query_cov": 0.9731543624161074, "subject_cov": 0.847953216374269, "identity": 0.503, "score": 135.0, "evalue": 1.28e-37, "valid": false, "product": "GspE/PulE family protein", "uniref50_id": "UniRef50_UPI00166D045C" }, "pscc": { "uniref50_id": "UniRef50_UPI00166D045C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00166D045C" ], "product": "GspE/PulE family protein" }, "genes": [], "id": "NKOCPLAKPK_1538", "locus": "GCA003260355_01507" }, { "type": "cds", "sequence": "contig_1", "start": 814360, "stop": 814623, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00437.27" ], "nt": "TTGTTTAATGAAACAGATAATGGTATGGAAACTGTACAATTTAAGGCACAATTACAGCAATCTATCCTTAGGTCAGATATCGTGAAGATTTTTGACAATCTTTTGACAATTGCTGTAGAAGAAGGTGCATCAGATATCCATATTGAACCACTAGAAAATTACTCAAGAATTAGACTAAGAATAGATGGAGTCCTACAAGAACTCATCCAATATCCTCGTTCTCTTCATGAATCAATAATTTCCAAGTTTAAAATTGAATCTTGA", "aa": "MFNETDNGMETVQFKAQLQQSILRSDIVKIFDNLLTIAVEEGASDIHIEPLENYSRIRLRIDGVLQELIQYPRSLHESIISKFKIES", "aa_hexdigest": "8377d1899e09af97cebb9fd432555e72", "start_type": "TTG", "rbs_motif": "TTTA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00437.27", "name": "T2SSE", "length": 61, "aa_cov": 0.7011494252873564, "hmm_cov": 1.0, "evalue": 5.466681116292547e-13, "score": 44.720176696777344, "start": 26, "stop": 86 } ], "seq_stats": { "molecular_weight": 10077.375499999993, "isoelectric_point": 4.87521686553955 }, "id": "NKOCPLAKPK_1539", "locus": "GCA003260355_01508" }, { "type": "cds", "sequence": "contig_1", "start": 814861, "stop": 814974, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00166.26" ], "nt": "ATGACAAGACTTATTCCATTAGAGGATCACATTATTGTGAAACCACTCGTCCAAGAATCAACAACAAAATCTGGGATTATCCTTCCAGAAAATAAAGAGAAGCCGAGCAAATGA", "aa": "MTRLIPLEDHIIVKPLVQESTTKSGIILPENKEKPSK", "aa_hexdigest": "e24b429629250bea8ecf0bc1171b4afe", "start_type": "ATG", "rbs_motif": "TTTTTA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00166.26", "name": "Cpn10", "length": 32, "aa_cov": 0.8648648648648649, "hmm_cov": 1.0, "evalue": 2.925196527451365e-08, "score": 30.1230411529541, "start": 4, "stop": 35 } ], "seq_stats": { "molecular_weight": 4183.909100000001, "isoelectric_point": 8.162422752380369 }, "id": "NKOCPLAKPK_1540", "locus": "GCA003260355_01509" }, { "type": "cds", "sequence": "contig_1", "start": 815053, "stop": 815160, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTATTTCGCAAAATATTCTATTGATGAGCTAGAAATTGATGAAGATGGAGAAAAAAAGAAATATCTTGTAATGAAGCAGAATTATATTATGGCAAAAGAGGCTTAA", "aa": "MYFAKYSIDELEIDEDGEKKKYLVMKQNYIMAKEA", "aa_hexdigest": "d7495b60f4c47b706366bc0abe20e4a8", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4235.849000000001, "isoelectric_point": 4.812637138366699 }, "id": "NKOCPLAKPK_1541", "locus": "GCA003260355_01510" }, { "type": "cds", "sequence": "contig_1", "start": 815360, "stop": 815593, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTTAAGTATATTTTGGAAGTATCTAAAAAGGATTATACTGATCGTGGATGTAAGGCGTCTGACATCTATCAAGCGGATACTGCATATCAGAATGATCTCAAAACTCTTTGTGGATTTGGTATTTTTATTGGGGAAAAGAATAAACTCCTTCCTAAGCAATACCTTTCAAATGCTCAGGCTATAACACTCGTAATGAGAGTCGTAGATGGCTATCAAAAGGAAGCCACCTGA", "aa": "MLKYILEVSKKDYTDRGCKASDIYQADTAYQNDLKTLCGFGIFIGEKNKLLPKQYLSNAQAITLVMRVVDGYQKEAT", "aa_hexdigest": "a139c0570d30b85a63ac6755c839fb86", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8729.985499999997, "isoelectric_point": 8.544913673400881 }, "id": "NKOCPLAKPK_1542", "locus": "GCA003260355_01511" }, { "type": "cds", "sequence": "contig_1", "start": 815684, "stop": 815842, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACATTGGAAAACTTCATTAACTTCCTTTATAGCGTAGAGCACCCTCATCAATCGGTTACTCAAACAGAAATGAGCGTAGACACCTCAAATCAGAAATCAAGTTTTAAATCATCAGACGATGCTCTACTGAAGCTCGCTGAGATTATGTCTAGTTAA", "aa": "MTLENFINFLYSVEHPHQSVTQTEMSVDTSNQKSSFKSSDDALLKLAEIMSS", "aa_hexdigest": "ddd2e381757905ffa26fa264435408fc", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5867.462799999998, "isoelectric_point": 4.685886192321777 }, "id": "NKOCPLAKPK_1543", "locus": "GCA003260355_01512" }, { "type": "cds", "sequence": "contig_1", "start": 815909, "stop": 816970, "strand": "+", "frame": 2, "gene": "groL", "product": "chaperonin GroEL", "db_xrefs": [ "GO:0042026", "GO:0140662", "SO:0001217", "UniRef:UniRef50_A1T577", "UniRef:UniRef90_A0A328EI13" ], "nt": "ATGCCAAAACACATTAAGTATGGTGATGAAGCCAGAAAAGAAATCTTTAGCGGAATGCAAATGGTTGCAGACGCCGTGAAAGTCACTATGGGACCAAAAGGAAGAAATGTAATCCTTGAAAGATCTTATGGAGGACCTATCGTAACGAATGATGGAGTGACCGTAGCCAAAGAAATTGAACTTGAAGACAAATCCGAAAACATCGGAGCAAGCATGCTCAAAGAAGCTGCTGAAAAAACCAATAAAGAAGCAGGAGATGGAACAACTACCACCGTTGTGCTTGCAGAGGCGATGGCGAGCGAAGGACTCAGATATATCAGGTCTGGAGTGAATCCTTTCTCTCTTGGGAAGGGGCTTCATAAGGCGGTTGAGAAGCTTGTAGCGGAGATTGAAGGGAAAGCCCAGCAAATCGGAGATAGTAAAGAGAAAATCAAGCAAGTAGCGACAATTTCTGCTCAAGATGAAGAAGTAGGAAATCTGATCGCTGATGTCTTTGAGGAGATCGGAAAAGACGGAACCATTACTGTTGAAGAGGGAAAATCTCTCGGTCTAACCAAAGAAATCAAAACAGGAATGCAATTTGATCAGGGATATTCTAGTCCTTACCTTGTAACTGATCCTCAAAGAATGGAAGCAGTAATTGATAAGCCTTATATTTTGCTTACCGACAAGAAAATTTCTTCTATCAAGGAGATTTTGCAGGTTTTGGAGGCAGTTGCTGCGACTGGGAAAAAGGATTTTGTGATTATTGCTGAGGATGTTGAGGGTGAAGCTCTGGCTTCTTTGGTTTTGAATAAAATCAGAGGAATGCTGAATGTTTTGACCGTGAAGGCTCCAGGATTCGGAGATAGGAAAAAGGAAATGCTTCGCGATATCGCGGTAGTAACTGGTGCAACAGTGATTACAGAAGAGCTTGGACTTAAACTAGAGGACGCTACGATTGAGATGCTTGGAAGAGCTGATAAAGTGATTGCGACCAAAGATACGACCGTAATCGTAGATGGTAAAGGCGATCAGACCGCTATCAATGAAAGAGCTGATCAGATTAAAGCACAGCTCTGA", "aa": "MPKHIKYGDEARKEIFSGMQMVADAVKVTMGPKGRNVILERSYGGPIVTNDGVTVAKEIELEDKSENIGASMLKEAAEKTNKEAGDGTTTTVVLAEAMASEGLRYIRSGVNPFSLGKGLHKAVEKLVAEIEGKAQQIGDSKEKIKQVATISAQDEEVGNLIADVFEEIGKDGTITVEEGKSLGLTKEIKTGMQFDQGYSSPYLVTDPQRMEAVIDKPYILLTDKKISSIKEILQVLEAVAATGKKDFVIIAEDVEGEALASLVLNKIRGMLNVLTVKAPGFGDRKKEMLRDIAVVTGATVITEELGLKLEDATIEMLGRADKVIATKDTTVIVDGKGDQTAINERADQIKAQL", "aa_hexdigest": "1c560b923f2b70b3298d64f78fe3c61d", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00257DDBCD", "query_cov": 1.0, "subject_cov": 0.8914141414141414, "identity": 0.575, "score": 395.0, "evalue": 8.88e-134, "valid": false, "gene": "groL", "product": "chaperonin GroEL", "uniref50_id": "UniRef50_A0A358M8W5", "go_ids": [ "GO:0042026", "GO:0140662" ] }, "genes": [ "groL" ], "truncated": "3-prime", "pseudogene": { "start": 815909, "stop": 817273, "observations": { "mutation": [ 816968 ], "stop": [ 816968 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EI13", "query_cov": 1.0, "subject_cov": 0.6394927536231884, "identity": 1.0, "score": 662.0, "evalue": 1.07e-236, "gene_start": 1, "gene_end": 353, "reference_start": 1, "reference_end": 353, "reference_sequence": "MPKHIKYGDEARKEIFSGMQMVADAVKVTMGPKGRNVILERSYGGPIVTNDGVTVAKEIELEDKSENIGASMLKEAAEKTNKEAGDGTTTTVVLAEAMASEGLRYIRSGVNPFSLGKGLHKAVEKLVAEIEGKAQQIGDSKEKIKQVATISAQDEEVGNLIADVFEEIGKDGTITVEEGKSLGLTKEIKTGMQFDQGYSSPYLVTDPQRMEAVIDKPYILLTDKKISSIKEILQVLEAVAATGKKDFVIIAEDVEGEALASLVLNKIRGMLNVLTVKAPGFGDRKKEMLRDIAVVTGATVITEELGLKLEDATIEMLGRADKVIATKDTTVIVDGKGDQTAINERADQIKAQLGMTTSDYDKEKLAERLAKLVGGVAVIQVGAATEMEMKNKKYKIEDALNATRAAIEEGIVAGGGSVLLQLSKLLDGFKLEDADEQVAVQIVRDAIQYPVRQIANNAGFKGDRVVEKVKESEDFNYGFDAKEGTFKNLFEAGIIDPAKVLRVALQNAVSTAAMFLTTESVIVDAPKAEGCSCSHGGADAGMGGMGGMGGMY" }, "paralog": false, "identity": 0.9977973568281938, "score": 857.0, "evalue": 1.39e-315, "description": "Internal stop codon at 816968. Nonsense mutation around 816968.", "psc": { "uniref90_id": "UniRef90_A0A328EI13", "gene": "groL", "product": "chaperonin GroEL", "uniref50_id": "UniRef50_A1T577", "go_ids": [ "GO:0042026", "GO:0140662" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EI13", "UniRef:UniRef50_A1T577", "GO:0042026", "GO:0140662" ] }, "pscc": { "uniref50_id": "UniRef50_A1T577", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A1T577" ], "product": "Chaperonin GroEL 1" } }, "id": "NKOCPLAKPK_1544", "locus": "GCA003260355_01513" }, { "type": "cds", "sequence": "contig_1", "start": 816971, "stop": 817537, "strand": "+", "frame": 2, "gene": null, "product": "60 kDa chaperonin", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3R7PK82" ], "nt": "ATGACCACTTCTGATTATGATAAAGAAAAATTAGCTGAAAGGCTGGCTAAACTCGTTGGTGGAGTGGCGGTTATTCAGGTCGGAGCTGCAACTGAAATGGAGATGAAAAACAAGAAATATAAGATTGAAGATGCTTTGAATGCAACTAGAGCCGCTATTGAAGAGGGAATCGTTGCTGGAGGGGGAAGCGTATTGCTTCAGCTTTCAAAGCTTTTGGATGGCTTCAAACTAGAAGATGCAGATGAACAAGTTGCTGTGCAGATCGTGAGAGATGCGATCCAATATCCTGTTCGCCAAATCGCAAATAATGCAGGTTTCAAAGGAGATCGAGTAGTAGAAAAAGTAAAGGAGTCTGAAGATTTCAACTACGGTTTTGATGCCAAAGAAGGAACATTCAAAAACCTCTTTGAAGCAGGGATTATTGATCCAGCAAAAGTGCTGAGAGTTGCACTTCAGAACGCGGTTTCTACTGCTGCGATGTTCCTTACGACTGAAAGCGTGATCGTAGATGCTCCAAAGGCAGAAGGCTGCTCTTGCTCTCACGGAGGAGCTGATGCAGGAATGTGA", "aa": "MTTSDYDKEKLAERLAKLVGGVAVIQVGAATEMEMKNKKYKIEDALNATRAAIEEGIVAGGGSVLLQLSKLLDGFKLEDADEQVAVQIVRDAIQYPVRQIANNAGFKGDRVVEKVKESEDFNYGFDAKEGTFKNLFEAGIIDPAKVLRVALQNAVSTAAMFLTTESVIVDAPKAEGCSCSHGGADAGM", "aa_hexdigest": "b57f93a795af530e3d2db543df9e0e67", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002734BB93", "query_cov": 0.9946808510638298, "subject_cov": 0.9303482587064676, "identity": 0.588, "score": 206.0, "evalue": 1.24e-64, "valid": false, "product": "TCP-1/cpn60 chaperonin family protein", "uniref50_id": "UniRef50_A0A3R7PK82" }, "pscc": { "uniref50_id": "UniRef50_A0A3R7PK82", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3R7PK82" ], "product": "60 kDa chaperonin" }, "genes": [], "id": "NKOCPLAKPK_1545", "locus": "GCA003260355_01514" }, { "type": "cds", "sequence": "contig_1", "start": 817740, "stop": 818078, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03054.22" ], "nt": "TTGCAACAAGAGGGACATGAGGTCGTTGCCGGCTTTATGAAAAACTATGTTTCTGACAGAGGAAACTGTACTACGTATAATGATTCGGTTGACGCAATAAAGGTGGCTGAATTTTTAGGGATTGAAATTCGCTCTTTTGACCTTCAAAAAGAGTATCAGGAGAGAATTATTGACTATATTTATGCGGGATATCAGAAAGGTATTACTCCGAACCCAGATGTACTTTGTAATTCTTTGATCAAGTTTGATGTTTTTTTGGAGAGAGCTTTGGAACAAGGTTTTGACAAGATTGCAACCGGTCACTATGCTAGAATTAAAGAATATCAAAAAGAAGATTGA", "aa": "MQQEGHEVVAGFMKNYVSDRGNCTTYNDSVDAIKVAEFLGIEIRSFDLQKEYQERIIDYIYAGYQKGITPNPDVLCNSLIKFDVFLERALEQGFDKIATGHYARIKEYQKED", "aa_hexdigest": "3f0fe79c469df7f2277c1e4de6ab89fb", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03054.22", "name": "tRNA_Me_trans", "length": 115, "aa_cov": 0.9732142857142857, "hmm_cov": 1.0, "evalue": 3.9117356212514746e-35, "score": 117.7864761352539, "start": 1, "stop": 109 } ], "seq_stats": { "molecular_weight": 12955.400499999982, "isoelectric_point": 4.884197425842285 }, "id": "NKOCPLAKPK_1546", "locus": "GCA003260355_01515" }, { "type": "cds", "sequence": "contig_1", "start": 818085, "stop": 818717, "strand": "+", "frame": 3, "gene": null, "product": "tRNA-uridine 2-sulfurtransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2M7XJD8" ], "nt": "ATGTTTCATCTCCTTCGTGGAGTGGACCACCATAAGGATCAATCTTACTTTCTCGCTGGGTTGAATCAATTTCAGCTTCAGCATTCGCTTTTCCCTCTTGGAGAGCTCACAAAACCGGAAGTGAGAGCGATTGCTGAAAAAATTGGATTGCCAAACGCCTACCGTAGAGATTCTCAAGGGCTCTGTTTTGTGGGTAACATTCCGATTAAACAATTTCTTGCACAGAAATTTCCTATTAACAAAGGGGAAATAAGAACCTTGGAGGGAAAAACGGTAGGAACTCATGATGGAGCTTACTTTTTTACGATAGGGCAAAAACATGGATTAGGACTGAATTTTAAAGCTTATGTGTATCGTATTGATGTAAAGGAGAACATCGTCTATGTTTGCAATAAAGATGCTGATGAGCTTCAAACTCAGGAGATTACCGTGAAAGATTGGCATCGAATCGGTGAGGCTTATCCAGAAGGCGAAAAACTTACTGGGAAAATTCGCTATCGTCAGAATCCCCCTGTTGATTGTTTATTATTAAAAAGAAGAGAATCTGAATTAATTATTCGTTTTGATTTTCCTCAGCGGGCAGTTGCTCCTGGTCAGGTTTTTGTCTTGTATAATGGAGAAACCTGCCTATGA", "aa": "MFHLLRGVDHHKDQSYFLAGLNQFQLQHSLFPLGELTKPEVRAIAEKIGLPNAYRRDSQGLCFVGNIPIKQFLAQKFPINKGEIRTLEGKTVGTHDGAYFFTIGQKHGLGLNFKAYVYRIDVKENIVYVCNKDADELQTQEITVKDWHRIGEAYPEGEKLTGKIRYRQNPPVDCLLLKRRESELIIRFDFPQRAVAPGQVFVLYNGETCL", "aa_hexdigest": "560dc0813164fc6f60817c3b2f19fdc7", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A2M7XJD8", "query_cov": 1.0666666666666667, "subject_cov": 0.8057553956834532, "identity": 0.513, "score": 214.0, "evalue": 2.93e-66, "valid": false, "product": "tRNA-uridine 2-sulfurtransferase", "uniref50_id": "UniRef50_A0A2M7XJD8" }, "pscc": { "uniref50_id": "UniRef50_A0A2M7XJD8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2M7XJD8" ], "product": "tRNA-uridine 2-sulfurtransferase" }, "genes": [], "id": "NKOCPLAKPK_1547", "locus": "GCA003260355_01516" }, { "type": "cds", "sequence": "contig_1", "start": 818914, "stop": 819240, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAATCAGAAGAATAAGAAGATTGAAATCAGAAAGGTTAACAAAACCGAATATCCTTTCCTTTATTGCGTACACCTTAATTTTGTTTTTTGGAGTTACGATCTTGGTAAACATTAACCCTAATGCAAATGAATCTTATCGCAATAAGCGAGATGAGAAAAATACTCAGGTGATGCAATGGATTTTTTTGGAAGAAAATGGACTTTCTCATATACTTTTTGGGGAGACAGTTACTGGAGAACATGCCGCTCCACAAACTCGAGACTTTCTTCTTCCAAAGTCTAGTATAGAACAGCAAGAAAGTAGTCCTGAGGTTGAGAAATGA", "aa": "MAIRRIRRLKSERLTKPNILSFIAYTLILFFGVTILVNINPNANESYRNKRDEKNTQVMQWIFLEENGLSHILFGETVTGEHAAPQTRDFLLPKSSIEQQESSPEVEK", "aa_hexdigest": "d347c88d2cb80af9d0cbe797208f4acc", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12518.180999999995, "isoelectric_point": 8.078484916687014 }, "id": "NKOCPLAKPK_1548", "locus": "GCA003260355_01517" }, { "type": "cds", "sequence": "contig_1", "start": 819451, "stop": 819609, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTTTTGAATATTTGATCAGAGGAGATATTGTAGAGCTTCCTGAAGGGGCGGTCAGATATGTCGTTTTACTCTATGGAATGGATGATAAAGTTAAAGAGAAAGTTTTTGAGGCAAAGTCCTGCATCACACCTCGAGGATATGAAATCGCTCATTGA", "aa": "MIFEYLIRGDIVELPEGAVRYVVLLYGMDDKVKEKVFEAKSCITPRGYEIAH", "aa_hexdigest": "7b7cf59e972c27839c82a6ac1eccc970", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6005.9773, "isoelectric_point": 5.1876039505004865 }, "id": "NKOCPLAKPK_1549", "locus": "GCA003260355_01518" }, { "type": "cds", "sequence": "contig_1", "start": 819593, "stop": 819940, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATCGCTCATTGAGGATCTGTACTTGCCTATGAACAGAGAGCTGATGCTCCCTATGTTTGCAATCTTGAAAGGAGAACCTGCAAAAACTGAAAACTTTCTGGTAGTTTTACTCAAGCCTCTTGCGATGAAAATCTCAATGGGAATATCAAAAAGTATTCTCACATTACGACGAACACTGAAGCAGCGATCACTACCAATGAATATATTCAACCTGATAAAAAGGCAAAATTTGAGGATGCTGATTTTAATTTGCAGGGGAAGCGTGCACCTAACGAAAAACCTCACCTTATTTCTTGACCACTTAGAAATGAGACAAGTGCAACTCCAAATGATGTTCCATTAG", "aa": "MKSLIEDLYLPMNRELMLPMFAILKGEPAKTENFLVVLLKPLAMKISMGISKSILTLRRTLKQRSLPMNIFNLIKRQNLRMLILICRGSVHLTKNLTLFLDHLEMRQVQLQMMFH", "aa_hexdigest": "3c8215c68efaa80265452572e8276414", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13507.488699999984, "isoelectric_point": 10.53975887298584 }, "id": "NKOCPLAKPK_1550", "locus": "GCA003260355_01519" }, { "type": "cds", "sequence": "contig_1", "start": 820404, "stop": 820562, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAGTTATTCCTTCCTTAGCCTTCTCTTTTTTAATATCATATTAGGCGTGGCCATTACCATTTTTGGAATTGGGCTGATTATCTCCGGCTTCCAGCTCAAAGAGGGGGAGGTGAAGAGTATCGAGTCAGATCCTTCAGAATCGCTTCCCATAGCATAA", "aa": "MSYSFLSLLFFNIILGVAITIFGIGLIISGFQLKEGEVKSIESDPSESLPIA", "aa_hexdigest": "3885fe47644cfbd6a6e6fe48246f9e6a", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5606.521899999999, "isoelectric_point": 4.251295280456543 }, "id": "NKOCPLAKPK_1551", "locus": "GCA003260355_01520" }, { "type": "cds", "sequence": "contig_1", "start": 820866, "stop": 820979, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTAAGATCAAAGCTTTCTTCCGAATTAATGGTATTATTTTAATGCTATTAGGGCTTTTCCTGTTTTTTCAGCCAGAACTTGCACTAAGAGTTGTTATTATTATTTTCTGA", "aa": "MLKIKAFFRINGIILMLLGLFLFFQPELALRVVIIIF", "aa_hexdigest": "54d7a07bf1c1db0797dbf342e6b81479", "start_type": "ATG", "rbs_motif": "TTTA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4352.5115, "isoelectric_point": 11.000127601623536 }, "id": "NKOCPLAKPK_1552", "locus": "GCA003260355_01521" }, { "type": "cds", "sequence": "contig_1", "start": 821114, "stop": 821233, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAACTAAAGAAAATTGTAATGGGAATTTTATTCGCGACACTGATTATTGTCGGAATAGAAGGGATGATCAGTTTTTGGGAGAATCATAGCTACTATACGGCAATGATTTTGTCCTGA", "aa": "MKLKKIVMGILFATLIIVGIEGMISFWENHSYYTAMILS", "aa_hexdigest": "ad65d2c7188eafbce485565a994d6c5b", "start_type": "ATG", "rbs_motif": "TTATT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4463.4349999999995, "isoelectric_point": 8.161391258239746 }, "id": "NKOCPLAKPK_1553", "locus": "GCA003260355_01522" }, { "type": "cds", "sequence": "contig_1", "start": 821447, "stop": 821752, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGCAATAGGCTTCATTCTTGCTGTTTTTTCGCTTGGGAACTTCATATCAAGGAAGCTAAAGCTTTTTCAAAAGATAAGATGGCAAGAAACGCTTCTCACCTTTGGACTGGGACTCGTGACTTTTCTTCTCCTGATGCAAATCCTGATGGGAATTCAGCTTTTTTATAGTGTCGTAAATCGAGTAATTTTTGGAGGGCTTCTCGTGCTTATGCGATTCGAGAGAAAGGCTATGAAAGTTCATGAAGAGAATCTTATTTCTTGTCTAGAAAGCTGGAAATCAGCGAATAAAGCTTCAAAAATCTGA", "aa": "MAIGFILAVFSLGNFISRKLKLFQKIRWQETLLTFGLGLVTFLLLMQILMGIQLFYSVVNRVIFGGLLVLMRFERKAMKVHEENLISCLESWKSANKASKI", "aa_hexdigest": "c9555e362cef957e7de553b5ccc856a3", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11612.012399999989, "isoelectric_point": 10.290975379943848 }, "id": "NKOCPLAKPK_1554", "locus": "GCA003260355_01523" }, { "type": "cds", "sequence": "contig_1", "start": 821858, "stop": 822064, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATGTCCCTAAGGTTATTGCCGAAAATGCGGGTATTCTTTGGGGGAATATAGGTCCTGCTAATGGAGTACAATATCTCTGGCAGGGATACATCTCCTTTTTCTTTTCTCTCTGGGCACCTCTCGGAAATTTCTTCAATATCGCAAAAGATACTGTTGCTATTAACCTTAATGCTTTTAGCGGGGTTCTCGTGTTAATCTTTTGA", "aa": "MYVPKVIAENAGILWGNIGPANGVQYLWQGYISFFFSLWAPLGNFFNIAKDTVAINLNAFSGVLVLIF", "aa_hexdigest": "c6c9f904003f43d2806d208fa997d06e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7512.677999999997, "isoelectric_point": 5.822381782531737 }, "id": "NKOCPLAKPK_1555", "locus": "GCA003260355_01524" }, { "type": "cds", "sequence": "contig_1", "start": 822071, "stop": 822676, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCTCAAAGAAGCACTCCTTGTCTTCGGAAGAAAGGAATCCTCTGAAGAAACTGATCAGACTTCTTTCCTTGTAGGACGAGGACTTCTTCTGATGTGGCTTACAAGTGGGATGGGAGCATTTCTCGTGTTTGTAGATAATAAGACCGATATGGGGGTTCTTGCACTGACGATCCTTGCTTTGTTTGGAGGGATGATTTTTCTTAACCATTTCCAAAAGAAGGGAATCGATACTGATTCAAAACAAAGTTTGAAATATCTTATTCTCTCTGCTCTCTTCTTTGGATTCGCTGTTATGGCAAAACCAACCGCTTTTATTGATGTTGTGATCTTTGCATTGTTACTTGTTGGCTTTTGGCTCAATACAACAACCCTTGTAGGAACCGGCATTGCAACACTTGGGCTTATGGGAGTTGTGCAACCACTTTTTGCTTCAGCATTCATTACTCCTGAGCTGGGGAAATGGCTCTTCGCTATCGGAATAGTACTTGCTGTCATTGGAGGAATCCGCGGATTAATCTCAAGAAATGATCAGTTTTCAAAAGGATTTCAGCATATTGTAATCTGGGGAATTACACTCATTGCAACGCTATTTCTTTTCAAGTGA", "aa": "MLKEALLVFGRKESSEETDQTSFLVGRGLLLMWLTSGMGAFLVFVDNKTDMGVLALTILALFGGMIFLNHFQKKGIDTDSKQSLKYLILSALFFGFAVMAKPTAFIDVVIFALLLVGFWLNTTTLVGTGIATLGLMGVVQPLFASAFITPELGKWLFAIGIVLAVIGGIRGLISRNDQFSKGFQHIVIWGITLIATLFLFK", "aa_hexdigest": "07c399de859336a34b2d1ab8aec06cdc", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 21917.979399999993, "isoelectric_point": 9.30054759979248 }, "id": "NKOCPLAKPK_1556", "locus": "GCA003260355_01525" }, { "type": "cds", "sequence": "contig_1", "start": 822821, "stop": 823003, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAGAATCAATACCGATAGACAGTCTGATATTGATCGTGCAGTGCTTAATCGTGCGACTCAAGATCTTGACTATCAGCAATGCCTCAAAGAAGCAAACGACCCTCAAGAGCTTGATAGAACAAAGCAACAAGCTCCTGGAAGCTCTCTAAGTGAGGATGTGGGAAGATATGTAGGATTTTGA", "aa": "MRINTDRQSDIDRAVLNRATQDLDYQQCLKEANDPQELDRTKQQAPGSSLSEDVGRYVGF", "aa_hexdigest": "522edc2d647082fc2ff530870b752c9d", "start_type": "GTG", "rbs_motif": "TTCTT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6857.3996000000025, "isoelectric_point": 4.61614475250244 }, "id": "NKOCPLAKPK_1557", "locus": "GCA003260355_01526" }, { "type": "cds", "sequence": "contig_1", "start": 823058, "stop": 823231, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAGAAAATACTTGCTTTGGTTGGGATAGTGATGGACTTCTCCTCTGCAAGAATCGTAACCTTTTGGACGCGAAAAATCTCTCTGAGATTGAAGCACTTTCGTCTCAACTTTCTAAAAATGGTGCTGCTCAAGCTTTGGTGAAATCGCTTCTAGATGCACAAGCCTCATGA", "aa": "MKENTCFGWDSDGLLLCKNRNLLDAKNLSEIEALSSQLSKNGAAQALVKSLLDAQAS", "aa_hexdigest": "75f67a7e176f53057e638e3ad72bb27d", "start_type": "TTG", "rbs_motif": "TTTA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6124.886600000002, "isoelectric_point": 5.042494010925293 }, "id": "NKOCPLAKPK_1558", "locus": "GCA003260355_01527" }, { "type": "cds", "sequence": "contig_1", "start": 823436, "stop": 823738, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTCTATGCAATCCTTAGCTATGATAGGAAGTATAAGCATCTACTCTTTCTCAGCTTTGGAACGGTAATCGGGTGGATCATTTGGTGGGCTATTGGCGGAGGAATCCTCTGGTATGGATTAGGTCTTGTGATTTGGTCTACGCTCACTGTCACGGCACTCTTGCAGGAATGGGCCCCTAAAAAAGAAAGTGGCATAGAGTTTAAAACAATATATGGGCTGCTTGTTGGTTTCTTGGCAATCTTCCTTGCTATCCAGGCATTTATGAATGGTATGAGGATTGCTTCTCAGGCAGGTGAATGA", "aa": "MLYAILSYDRKYKHLLFLSFGTVIGWIIWWAIGGGILWYGLGLVIWSTLTVTALLQEWAPKKESGIEFKTIYGLLVGFLAIFLAIQAFMNGMRIASQAGE", "aa_hexdigest": "4a1f3a02e315c5a0730fa8747f014225", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11262.294399999992, "isoelectric_point": 9.049443244934082 }, "id": "NKOCPLAKPK_1559", "locus": "GCA003260355_01528" }, { "type": "cds", "sequence": "contig_1", "start": 823847, "stop": 824431, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAGTTTGGACAGTATCAGCCATTTCTTGAGGCGGTAAAGAATAGAACTGCTGATGAAGGCGTCCTTATTGCCGGAACCTACCTTCAATACTTCTTGAATAATCAGAAAAATCTGAGACTCGATGGTATGCTGACTCGACTCTGGGAAGAGCTGTCAGACTTTAATAGCTGTAGATCCTACCAAAGACTTAAAAATGAGAAACTCAAATATCTTATCATTGATCCAAATATTGGAACGGTTGGAAGAGCAGGAGAAGGAAATGAAAGCCTTTTCTATAGGTTCTTTGCGAGACTCACCTCAGATGAGCAGCACATTGAAACTCATGGAACGATCAGTATGCTCGTAAAGATGGCACAGGAAGGCTACCTCAAGCTTATTTTTACAAATAATATTGGTGCAAAGTATGCCTTTACACTCTCTGATGAAGAGTTAACAAGTGTTTTTGGAACTCACTCTGCTGAAGATCTGCTCCTGATTAGAGCAAAAATGGCGGTTGCTAGGTTCTTCTCTGAGGATCAAGATCTCATTTCCAAAATCTATGCCCTCTTTGGACAGAGACTTCCTACACAGAAAGGGCTCTGA", "aa": "MQFGQYQPFLEAVKNRTADEGVLIAGTYLQYFLNNQKNLRLDGMLTRLWEELSDFNSCRSYQRLKNEKLKYLIIDPNIGTVGRAGEGNESLFYRFFARLTSDEQHIETHGTISMLVKMAQEGYLKLIFTNNIGAKYAFTLSDEELTSVFGTHSAEDLLLIRAKMAVARFFSEDQDLISKIYALFGQRLPTQKGL", "aa_hexdigest": "233a55af67983b3718bb65c7faa15e72", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 22245.1428, "isoelectric_point": 6.319211387634279 }, "id": "NKOCPLAKPK_1560", "locus": "GCA003260355_01529" }, { "type": "cds", "sequence": "contig_1", "start": 824459, "stop": 824686, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGATGAAGAAAAACTCTACCAAATCTTCTTACAGATGGCTGGAGGTCAATTAACAAATGCCTCAAGTTTGACTCAGGACGAAAGAATTGTAGTAAGTCAGTATTACGGTCTAATAAAAATGTTGAGCGATAAGACAACCACTGAACAAGGACAGAATGCACTGTTTAGTCTCTTCCAGAAGAGTCTCGCAGGAAGCAGTCAAATTATTTCTCTTGAGCTAAGATAA", "aa": "MDEEKLYQIFLQMAGGQLTNASSLTQDERIVVSQYYGLIKMLSDKTTTEQGQNALFSLFQKSLAGSSQIISLELR", "aa_hexdigest": "85deb82769d2a094c107d47e63491f8a", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8407.471999999998, "isoelectric_point": 4.812637138366699 }, "id": "NKOCPLAKPK_1561", "locus": "GCA003260355_01530" }, { "type": "cds", "sequence": "contig_1", "start": 825176, "stop": 825697, "strand": "+", "frame": 2, "gene": null, "product": "KOW domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MQM4", "UniRef:UniRef90_A0A660MQM4" ], "nt": "ATGATAGTAGAGGTATTTGATTTTGGTTCCTCTGAACAGCTAGAAAGTATGGAGATACGCTATCAGACTTTGGAAGAAGCAGATCTTCTCATTCCTGCTACAGCTGTAGGAAGTTATATGCCAAATGTTTTGCGAGCAGCAATTGCTGGGGATGCTGAGGGAGTGAAGGTTATTACTCGACATATCCGAGATATTCCTGGCGAACCTATCACTAAGCTGATCGTTCTCCTGCAACGTTTCGGCTATCAGAAAATCAAGACAGCTCCGCTTATGCTTCCTGAAAAGAACGATATTATCGTTTACCATGGCAACTATGGTCACGCTATTGATACTGATCGTGGTTGGATTGGTATGATTACTAAGGTTTCTGTCAAGCATGGGCAACTCAAGGTTTGTGCGATCGGAGCTCAACGCTGGAATGTTCAAGGATCGGCCGGAGATGAGCAGTATGTAAGCCTGATCACCTCTTATCGACTAAACCTTAGCAAAGTAAGTGTTTATCGGGGATACTCCTCTCGGTAA", "aa": "MIVEVFDFGSSEQLESMEIRYQTLEEADLLIPATAVGSYMPNVLRAAIAGDAEGVKVITRHIRDIPGEPITKLIVLLQRFGYQKIKTAPLMLPEKNDIIVYHGNYGHAIDTDRGWIGMITKVSVKHGQLKVCAIGAQRWNVQGSAGDEQYVSLITSYRLNLSKVSVYRGYSSR", "aa_hexdigest": "281512f66a2cec03e0c592c67409350f", "start_type": "ATG", "rbs_motif": "TTTTT", "psc": { "uniref90_id": "UniRef90_A0A660MQM4", "query_cov": 0.9942196531791907, "subject_cov": 0.8557213930348259, "identity": 0.948, "score": 324.0, "evalue": 1.31e-111, "valid": true, "product": "KOW domain-containing protein", "uniref50_id": "UniRef50_A0A660MQM4" }, "pscc": { "uniref50_id": "UniRef50_A0A660MQM4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MQM4" ], "product": "KOW domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_1562", "locus": "GCA003260355_01531" }, { "type": "cds", "sequence": "contig_1", "start": 825728, "stop": 825952, "strand": "-", "frame": 3, "gene": null, "product": "DUF4234 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MP07", "UniRef:UniRef90_A0A328EHL7" ], "nt": "ATGAAGTCTGCTTCCTTCATGCAAGCAGTCACAAATAGCGTGCACGCTCAGTTTCCAAAGCCTAGAGTAAAATTAATAATTCTGACTTCAGTTTTTTTTTACTTTATTTTTTGTATGGTGAATCTATGGATTTTTGATGAAATGTCAAGAGAAGCTAAAAGGTTTTTTGTTTCCGATCACTATCAAATACCTACAACTCTTCAAAAAAAGAAAAAACTCTCCTAG", "aa": "MKSASFMQAVTNSVHAQFPKPRVKLIILTSVFFYFIFCMVNLWIFDEMSREAKRFFVSDHYQIPTTLQKKKKLS", "aa_hexdigest": "396cbf6ab92d1099b88e930a5b6ef123", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A328EHL7", "query_cov": 1.0, "subject_cov": 0.8131868131868132, "identity": 1.0, "score": 147.0, "evalue": 5.63e-45, "valid": true, "uniref50_id": "UniRef50_A0A660MP07" }, "pscc": { "uniref50_id": "UniRef50_A0A660MP07", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MP07" ], "product": "DUF4234 domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_1563", "locus": "GCA003260355_01532" }, { "type": "cds", "sequence": "contig_1", "start": 826065, "stop": 826586, "strand": "+", "frame": 3, "gene": null, "product": "Lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MMW8", "UniRef:UniRef90_A0A660MMW8" ], "nt": "ATGGTGATGGGGTTCTGTTTGAATTCCTGCAAAGAAAGAGATGTTTTACATTATTCTTTCAGTTCTTATGAGCGATCGCAGTCGCTTATTCCTCCCTGTTCTCAGGATGTTAACATTCCAAAGTGGCTGACAAGTAGCATTGCTGGCAATCCTGACTGTATTGATGTGGCCTTACATCTACTAAAGCTTGATCATCAGATGAAACCTATCGATTTCAAGCCGATATTGGAGCAGTACAACTATGAAGAGCCTAAATTGAAGTCTAGGAATGATTTAAGAGAAGGGGATATCATTCTCTATCATCTTGACTACGGGGAAGCGATTAACCCTGAGTGGGGCTGGATTGGTGTCGTCTACGACGTTTCTTTTGAGAATAATGGAGTAGCGCGTATTAGCTCTGTCGGTGCATGTCGCTGGAACTATAAAGCAACTGCCAACGAAGGTGAGATGGTATGTCTGCTCACCTCATATCATCTGAATATCCGAAAAATTAGTGTTTACCGGTGGAAGGATCCCCAATGA", "aa": "MVMGFCLNSCKERDVLHYSFSSYERSQSLIPPCSQDVNIPKWLTSSIAGNPDCIDVALHLLKLDHQMKPIDFKPILEQYNYEEPKLKSRNDLREGDIILYHLDYGEAINPEWGWIGVVYDVSFENNGVARISSVGACRWNYKATANEGEMVCLLTSYHLNIRKISVYRWKDPQ", "aa_hexdigest": "351e6df34a857ecd657e07e6c1f5230e", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 826023, "stop": 826700, "observations": { "mutation": [ 826584, 826608, 826632, 826671 ], "stop": [ 826584, 826608, 826632, 826671 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A660MMW8", "query_cov": 1.0, "subject_cov": 0.7688888888888888, "identity": 0.9420000000000001, "score": 344.0, "evalue": 3.61e-119, "gene_start": 1, "gene_end": 173, "reference_start": 15, "reference_end": 187, "reference_sequence": "MKTNKISFVSIWIMMVMAFCLNSCKERDVLHYSFSSYERSQSLIPACSQEGHIPKWLTSSIAGNPDCIDVALHLLKLDPQMKPIDFKPILEQYSYEEPKLKSRNDLREGDIILYHLDYGEAINPERGWIGVVYDVSFENNGVTRISSVGACRWNYKATVNEGEMVCLLTSYHLNIRKISVYRWKDPQGLSEKRTPWAFLEEFFGSLKRLENEKPVKGKNYLKALD" }, "paralog": false, "identity": 0.9288888888888889, "score": 427.0, "evalue": 3.74e-154, "description": "Internal stop codon at 826584, 826608, 826632, 826671. Nonsense mutation around 826584, 826608, 826632, 826671.", "psc": { "uniref90_id": "UniRef90_A0A660MMW8", "product": "Lipoprotein", "uniref50_id": "UniRef50_A0A660MMW8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A660MMW8", "UniRef:UniRef50_A0A660MMW8" ] }, "pscc": { "uniref50_id": "UniRef50_A0A660MMW8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MMW8" ], "product": "Lipoprotein" } }, "id": "NKOCPLAKPK_1564", "locus": "GCA003260355_01533" }, { "type": "cds", "sequence": "contig_1", "start": 827024, "stop": 827584, "strand": "+", "frame": 2, "gene": null, "product": "Peptidase C51 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBFF759", "UniRef:UniRef100_A0A328EJL4", "UniRef:UniRef50_A0A660MML0", "UniRef:UniRef90_A0A328EJL4" ], "nt": "ATGAGACCTAACAAAAATTTAACAATCTCTTTCGTGATTTTGGTAACCGTATGTCTACTTCTCAGTTCCTGCTGGAAAGAAGACGTGACTACTATCACCCGCATTCCTCAGAAGCAAGCTGCTAGCATGCTTGAGGTCTCTTCTGTTCAAGAACTGGCGCCTCAAATTTTGAGGGGTGCCGTTATTGGGAACGCTTACTGCGTTGTCTATCTTCGAAACTCCTTCAAGCTTGATAGTGCAGTATCTCTCAACCAGTTTGTTTCGGTTCTGAAGCACTGGGGGTATGTTTCTTACCCTGTTTCTCTTCGTGATATTCCTAAAAAGGGAGATTTCATCTTCTATCAGCCTGATTATGGAGCTGGAGTTCCCAATGAAGGATGGGTTGGCATCGTAATTGATGCTAACATAGTCAATAGAGATGAAATTCAGGTTCGCTCTATCTGTATTGGCGGCAAATCCCTTGAGCTATTATCGCTCTCAAAGGGCGACAACCTTCCGGTTATTACCTCTCGGAATCTTCTTCCGAGCAAAGTAATGGTTTACAGGAGCACTCGCTCCTAA", "aa": "MRPNKNLTISFVILVTVCLLLSSCWKEDVTTITRIPQKQAASMLEVSSVQELAPQILRGAVIGNAYCVVYLRNSFKLDSAVSLNQFVSVLKHWGYVSYPVSLRDIPKKGDFIFYQPDYGAGVPNEGWVGIVIDANIVNRDEIQVRSICIGGKSLELLSLSKGDNLPVITSRNLLPSKVMVYRSTRS", "aa_hexdigest": "b92ae6e2fe7d8cafc826f1ed324042e7", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000DBFF759", "uniref100_id": "UniRef100_A0A328EJL4", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBFF759", "UniRef:UniRef100_A0A328EJL4" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EJL4", "uniref90_id": "UniRef90_A0A328EJL4" }, "psc": { "uniref90_id": "UniRef90_A0A328EJL4", "product": "Peptidase C51 domain-containing protein", "uniref50_id": "UniRef50_A0A660MML0" }, "pscc": { "uniref50_id": "UniRef50_A0A660MML0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MML0" ], "product": "Lipoprotein" }, "genes": [], "id": "NKOCPLAKPK_1565", "locus": "GCA003260355_01534" }, { "type": "cds", "sequence": "contig_1", "start": 827903, "stop": 828175, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTCATCGCCCCTATGCTGTCTTCAGCGAGAGGATAGGTATCAAAAAGATCGTTTCCATACGTGTAGGCGTAGGGATAGTAGAGTTGTCAGGAAAGGATTTCGATTTGTATCCCCTCAGACTCGGAAAGTTTGAAGAAATCTGGATATTCCATCCTGATCGGAAAGACAGCATTCCTCTGCATTTGCTGTTCACGATCCATGCGGTAATCATCAAGGTTGATGATTCCTTCTTTAGAGAGGGGAATGATTTTGTCCTGATCAGATTTTGA", "aa": "MIHRPYAVFSERIGIKKIVSIRVGVGIVELSGKDFDLYPLRLGKFEEIWIFHPDRKDSIPLHLLFTIHAVIIKVDDSFFREGNDFVLIRF", "aa_hexdigest": "864a19ef9fe504e6071ea06c57949910", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10541.27949999999, "isoelectric_point": 8.111170387268068 }, "id": "NKOCPLAKPK_1566", "locus": "GCA003260355_01535" }, { "type": "cds", "sequence": "contig_1", "start": 828218, "stop": 828367, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAACGAAAGATCATTATTCCACGAGGATTATTTAGTATTACCTTGCTTGTCCTTGGCGCAACCTATTCTTTTGACGGCGTAAGGATGGGAATGGCGAAGCACCTCGCAGGAAGCCTCGAAGCTTACAAAATGGACCGACAACGCTGA", "aa": "MKRKIIIPRGLFSITLLVLGATYSFDGVRMGMAKHLAGSLEAYKMDRQR", "aa_hexdigest": "3b51ccaf542da9882d3a71d39373225e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5541.629300000001, "isoelectric_point": 10.60506534576416 }, "id": "NKOCPLAKPK_1567", "locus": "GCA003260355_01536" }, { "type": "cds", "sequence": "contig_1", "start": 828538, "stop": 828855, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EKQ4", "UniRef:UniRef90_A0A328EKQ4" ], "nt": "TTGCTTAATGGTATCAAAGATTACGCTCAGCATTCTCGCTCTGATAGGCTGGGATCTGGTGAAGCGAACAGGGGACGAAGGGAAGTTGCTCAAGCTAAGAGAGCTTTGGGTAATAAAACGCTCTGTCCTTCGGAGACCGCTCCCCCTTATGAGAGAAAAAAAGCCATGAGAGGTTGCTGTGGTCGTCTCTTTGGGAGGGGAAAGGGGGTAAAGCGTCCTTTCGGTTGCTATGACGGGTTGAGTGATGTCAGAAAGACCTCGTCTGGCATTGCTATCGTCAAAGCAACCCCAGAAAGACCTCCGCTGGCATCGCTATGA", "aa": "MLNGIKDYAQHSRSDRLGSGEANRGRREVAQAKRALGNKTLCPSETAPPYERKKAMRGCCGRLFGRGKGVKRPFGCYDGLSDVRKTSSGIAIVKATPERPPLASL", "aa_hexdigest": "036160d68eef74d20add3db50d2192d7", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A328EKQ4", "query_cov": 1.0, "subject_cov": 0.9722222222222222, "identity": 1.0, "score": 214.0, "evalue": 1.4e-70, "valid": true, "uniref50_id": "UniRef50_A0A328EKQ4" }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11376.979899999986, "isoelectric_point": 10.292071342468262 }, "id": "NKOCPLAKPK_1568", "locus": "GCA003260355_01537" }, { "type": "cds", "sequence": "contig_1", "start": 828976, "stop": 829335, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTCAGAAAGGAAGGCTCCTAAGGTTAAATTCTAAGGCTTCCCTCATGTATAATCACAGAGAAAGAAGCTTTTATCAGAATAAAAGACTGAATCTCTTCCAAAGAGTTGGCGAAATGCTGACTCTTTTTTCGTCCTCCACAACCCATCATAGCGACCGCAGGACCTCCTCAATCAAGCGAAGCGAAACGCAAACAACCGCATCGTGGCACTCCATTTCCTCTGCCACTCATCCTGAACAAGCTGATGCTAAAGCTGAAAGCAGAGCCAATCTCCCCGAAGGTAAAGCAACCTTTCCCGATAGGCAGGCGTTCGGACGAAATGTTGGAAAGAGTCATACAGAGGCTGCAGACAAGTAA", "aa": "MFQKGRLLRLNSKASLMYNHRERSFYQNKRLNLFQRVGEMLTLFSSSTTHHSDRRTSSIKRSETQTTASWHSISSATHPEQADAKAESRANLPEGKATFPDRQAFGRNVGKSHTEAADK", "aa_hexdigest": "e0848f01b1b9cbbcb058e5ed64957fde", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13552.913799999977, "isoelectric_point": 10.507008934020995 }, "id": "NKOCPLAKPK_1569", "locus": "GCA003260355_01538" }, { "type": "cds", "sequence": "contig_1", "start": 829298, "stop": 829861, "strand": "-", "frame": 3, "gene": "lemA", "product": "LemA family protein", "db_xrefs": [ "KEGG:K03744", "SO:0001217", "UniParc:UPI000DBF6461", "UniRef:UniRef100_A0A328ELJ2", "UniRef:UniRef50_I4A2E9", "UniRef:UniRef90_A0A328ELJ2" ], "nt": "ATGCCAACATCATTTACTCTAAATGCGTCCTGGATAATTATCGGGATTATCGTTATACTCGTTGTAATCCTGTATAACCGCTTGGTGAAGCTCAAAAATAATAGAGAGAACGCTTTCGCGGATATCGACGTTCAGCTCAAACTCAGATTTGATCTTGTAGAAAATCTCGTGAATACGGTTAAAGGTTACGCTACGCACGAACAGGAAACCTTTCAAAAAGTTATTGAAGCAAGGAATAGTTTCGCTCATGCAGGTTCTGTAGACAGCAAAATTGCTGCTGATAACCAGCTCACAGGAGCTCTTAAATCTATTTTTGCACTTGCTGAGAGTTATCCTGAGCTCAAGGCAAACGAAAACTTTCTCGAGCTTCAGACGGAGCTTTCAGATATTGAAAATAAGCTAGCTGCTGCAAGGAGATTCTTCAACTCTGCTACAAGGGAATACAATAATGCTATTCAGACCTTCCCAAGCAACTTCATTGCAGGAATGTTTGGTTTCAAAACTGAGAAAAGTTTTGAGTTAGACGATGTTTCAGAAAGGAAGGCTCCTAAGGTTAAATTCTAA", "aa": "MPTSFTLNASWIIIGIIVILVVILYNRLVKLKNNRENAFADIDVQLKLRFDLVENLVNTVKGYATHEQETFQKVIEARNSFAHAGSVDSKIAADNQLTGALKSIFALAESYPELKANENFLELQTELSDIENKLAAARRFFNSATREYNNAIQTFPSNFIAGMFGFKTEKSFELDDVSERKAPKVKF", "aa_hexdigest": "6d7539bcd88d3ac926ad85a7591dc3eb", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000DBF6461", "uniref100_id": "UniRef100_A0A328ELJ2", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBF6461", "UniRef:UniRef100_A0A328ELJ2" ] }, "ips": { "uniref100_id": "UniRef100_A0A328ELJ2", "uniref90_id": "UniRef90_A0A328ELJ2" }, "psc": { "uniref90_id": "UniRef90_A0A328ELJ2", "product": "LemA family protein", "uniref50_id": "UniRef50_I4A2E9", "kegg_orthology_id": "K03744" }, "pscc": { "uniref50_id": "UniRef50_I4A2E9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_I4A2E9" ], "product": "LemA family protein" }, "genes": [ "lemA" ], "id": "NKOCPLAKPK_1570", "locus": "GCA003260355_01539" }, { "type": "cds", "sequence": "contig_1", "start": 830046, "stop": 830444, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EHV1", "UniRef:UniRef90_A0A328EHV1" ], "nt": "ATGCCAGAAAAGAAGACAACAGCTTCGGCTCCTAAAGCAAAAGCTCCCAAGACTCCAGCAAAGAAACCAACCACTTCGACCCCAAAAACTAGGACAAAGACTGCTTCTGCGACGAGCACTTCTGCGACAAAAGCCAAACCTCAATCTACTCCAAAGCCAAGGAAAACATCGGCTCCAAAAGTAGCAGTGAAGACTAAACTAGAACGCAGTTTCTCTGTAAAGCAATTGGAAGAGGTAAGACCGTTGGAAGCCACTCCTGAGAAGAAGAAAGTCCCTACATGGATTGTGGTAGTATTTATCTTTTCACTTGTATTTTTCTTTTTTGCACTTTACAAGGCGTTTATCTATGGGCAGGGCTATGATCTTTCCCCTCTTGATACCTTCACTCCCTCTCAGTGA", "aa": "MPEKKTTASAPKAKAPKTPAKKPTTSTPKTRTKTASATSTSATKAKPQSTPKPRKTSAPKVAVKTKLERSFSVKQLEEVRPLEATPEKKKVPTWIVVVFIFSLVFFFFALYKAFIYGQGYDLSPLDTFTPSQ", "aa_hexdigest": "5bfaf201aa34d14d3c6472e28e622314", "start_type": "ATG", "rbs_motif": "TTCTT", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 830046, "stop": 830747, "observations": { "mutation": [ 830442, 830517 ], "stop": [ 830442, 830517 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EHV1", "query_cov": 1.0, "subject_cov": 0.44745762711864406, "identity": 1.0, "score": 204.0, "evalue": 1.45e-63, "gene_start": 1, "gene_end": 132, "reference_start": 1, "reference_end": 132, "reference_sequence": "MPEKKTTASAPKAKAPKTPAKKPTTSTPKTRTKTASATSTSATKAKPQSTPKPRKTSAPKVAVKTKLERSFSVKQLEEVRPLEATPEKKKVPTWIVVVFIFSLVFFFFALYKAFIYGQGYDLSPLDTFTPSQGKDAEISISNSSTDETTGVLVRSGTGVEANLADDPQLIQNFYSYLTNNQITEMNDLVDRPLKNSTAWLNHWNQKNIQIFTKHLADTVLLQDIFLIPGSINTQKQTRQYSYTLKYTIQPDHHFEEEWQVTLATRDGKTLISEIMCKTEGCSRSPFFWPQNYNLK" }, "paralog": false, "identity": 0.9871244635193133, "score": 409.0, "evalue": 1.38e-146, "description": "Internal stop codon at 830442, 830517. Nonsense mutation around 830442, 830517.", "psc": { "uniref90_id": "UniRef90_A0A328EHV1", "uniref50_id": "UniRef50_A0A328EHV1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EHV1", "UniRef:UniRef50_A0A328EHV1" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EHV1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EHV1" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 14538.819599999992, "isoelectric_point": 10.294779014587398 }, "id": "NKOCPLAKPK_1571", "locus": "GCA003260355_01540" }, { "type": "cds", "sequence": "contig_1", "start": 830550, "stop": 830933, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EHV1", "UniRef:UniRef90_A0A328EHV1" ], "nt": "TTGATTCAGAATTTTTATAGCTACCTCACGAATAATCAAATCACTGAAATGAACGATCTCGTTGACCGTCCGCTCAAAAATTCGACCGCTTGGCTCAACCATTGGAACCAGAAAAATATTCAGATTTTTACTAAACATTTGGCTGACACAGTGCTCCTCCAAGATATCTTCCTCATCCCTGGATCTATCAACACTCAGAAACAAACAAGACAATATTCTTATACTCTCAAATATACTATCCAACCCGATCATCACTTTGAGGAAGAATGGCAGGTAACTCTTGCTACTCGTGATGGGAAGACGTTAATCTCCGAGATTATGTGTAAGACTGAAGGCTGCTCTCGTTCTCCTTTCTTTTGGCCTCAAAACTACAACTTGAAATAA", "aa": "MIQNFYSYLTNNQITEMNDLVDRPLKNSTAWLNHWNQKNIQIFTKHLADTVLLQDIFLIPGSINTQKQTRQYSYTLKYTIQPDHHFEEEWQVTLATRDGKTLISEIMCKTEGCSRSPFFWPQNYNLK", "aa_hexdigest": "6d92e899dd9bb9a47c920c9dc8269ce3", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 830250, "stop": 830933, "observations": { "mutation": [ 830442, 830517 ], "stop": [ 830442, 830517 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EHV1", "query_cov": 1.0, "subject_cov": 0.43050847457627117, "identity": 0.992, "score": 270.0, "evalue": 1.96e-89, "gene_start": 1, "gene_end": 127, "reference_start": 169, "reference_end": 295, "reference_sequence": "MPEKKTTASAPKAKAPKTPAKKPTTSTPKTRTKTASATSTSATKAKPQSTPKPRKTSAPKVAVKTKLERSFSVKQLEEVRPLEATPEKKKVPTWIVVVFIFSLVFFFFALYKAFIYGQGYDLSPLDTFTPSQGKDAEISISNSSTDETTGVLVRSGTGVEANLADDPQLIQNFYSYLTNNQITEMNDLVDRPLKNSTAWLNHWNQKNIQIFTKHLADTVLLQDIFLIPGSINTQKQTRQYSYTLKYTIQPDHHFEEEWQVTLATRDGKTLISEIMCKTEGCSRSPFFWPQNYNLK" }, "paralog": false, "identity": 0.9911894273127754, "score": 458.0, "evalue": 3.84e-166, "description": "Internal stop codon at 830442, 830517. Nonsense mutation around 830442, 830517.", "psc": { "uniref90_id": "UniRef90_A0A328EHV1", "uniref50_id": "UniRef50_A0A328EHV1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EHV1", "UniRef:UniRef50_A0A328EHV1" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EHV1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EHV1" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 15133.96289999999, "isoelectric_point": 6.893569374084472 }, "id": "NKOCPLAKPK_1572", "locus": "GCA003260355_01541" }, { "type": "cds", "sequence": "contig_1", "start": 831027, "stop": 831536, "strand": "+", "frame": 3, "gene": "rlmN", "product": "23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN", "db_xrefs": [ "EC:2.1.1.192", "GO:0008757", "GO:0016433", "GO:0030488", "GO:0070475", "KEGG:K06941", "SO:0001217", "UniRef:UniRef50_A0A2W4ILJ4", "UniRef:UniRef90_A0A328EL21" ], "nt": "ATGATAGCCGTCTTTGAAGAAATGGTAGCACACAAACCAACGCTTTTTAATGAAGAACAACTGAATACCCGAGCAAAGCTTCACAAGCTCCAACCGTTCAAGGTCAAGCAAATTCTTTTTGAACTCTATAAGAACCAGAATACTGAACGAGATGCGATGACTACCCTTTCAAAGGAACTCAGGTCGCAGCTTCAGTCAGACTTTGATCTCTTGGCGATAGAACTCGTAGAAATGGTAGAAGCTGCAGATACTACGAAATTTGCGTTCAAAACGAGAGATGGGCACCTGATTGAGGCAATCCTTATGTATCACTGGCAGCATGAAAAATATGTTCAGAATCAAACTCCTAAACTTAACCGTATCACACTTTGTATTTCCTCTCAGGTCGGCTGTGCAATGCAGTGTCTTTTTTGTGTGACTGGGAAATTAGGGTTCAGGAAGAATTTGACCTGGGAAGAGATGATTTCTCAGGTGCTCTTTGCTAATAGCTATATCAAGAAGAGGTTCTGA", "aa": "MIAVFEEMVAHKPTLFNEEQLNTRAKLHKLQPFKVKQILFELYKNQNTERDAMTTLSKELRSQLQSDFDLLAIELVEMVEAADTTKFAFKTRDGHLIEAILMYHWQHEKYVQNQTPKLNRITLCISSQVGCAMQCLFCVTGKLGFRKNLTWEEMISQVLFANSYIKKRF", "aa_hexdigest": "618069a0e80c70fe4c1aa5e8d78f5110", "start_type": "ATG", "rbs_motif": null, "genes": [ "rlmN" ], "truncated": "3-prime", "pseudogene": { "start": 831027, "stop": 831839, "observations": { "mutation": [ 831534, 831696 ], "stop": [ 831534, 831696 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EL21", "query_cov": 1.0, "subject_cov": 0.4470899470899471, "identity": 1.0, "score": 337.0, "evalue": 6.14e-114, "gene_start": 1, "gene_end": 169, "reference_start": 1, "reference_end": 169, "reference_sequence": "MIAVFEEMVAHKPTLFNEEQLNTRAKLHKLQPFKVKQILFELYKNQNTERDAMTTLSKELRSQLQSDFDLLAIELVEMVEAADTTKFAFKTRDGHLIEAILMYHWQHEKYVQNQTPKLNRITLCISSQVGCAMQCLFCVTGKLGFRKNLTWEEMISQVLFANSYIKKRFGKKEDGTNYSVRNIVFMGMGEPLLNYDHTKMAIKLMLQQDRLSLGRRHVTISTCGIIPGIEQLIKDQIDVKLAISLHAPNQELRNQIMPIGKSYPLDKLMTTIDQYVEATNNRIFYEYIMIKDLTDTPALAHELVQLLKGRLAHINLIPYNENPAIHLQESSKLAIATFKQILESGGLTVTVRDSMGREAKGACGQLGYEKVKGQQLGF" }, "paralog": false, "identity": 0.9925925925925926, "score": 533.0, "evalue": 1.18e-193, "description": "Internal stop codon at 831534, 831696. Nonsense mutation around 831534, 831696.", "psc": { "uniref90_id": "UniRef90_A0A328EL21", "gene": "rlmN", "product": "23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN", "ec_ids": [ "2.1.1.192" ], "uniref50_id": "UniRef50_A0A2W4ILJ4", "kegg_orthology_id": "K06941", "go_ids": [ "GO:0008757", "GO:0016433", "GO:0030488", "GO:0070475" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EL21", "EC:2.1.1.192", "UniRef:UniRef50_A0A2W4ILJ4", "KEGG:K06941", "GO:0008757", "GO:0016433", "GO:0030488", "GO:0070475" ] }, "pscc": { "uniref50_id": "UniRef50_A0A2W4ILJ4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4ILJ4" ], "product": "Probable dual-specificity RNA methyltransferase RlmN" } }, "id": "NKOCPLAKPK_1573", "locus": "GCA003260355_01542" }, { "type": "cds", "sequence": "contig_1", "start": 831582, "stop": 831698, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGGATGGGAGAACCGCTTCTCAATTATGATCATACGAAGATGGCGATCAAGCTTATGCTCCAGCAAGATAGGCTTTCTCTAGGAAGGAGACATGTTACGATTTCTACGTGCTGA", "aa": "MGMGEPLLNYDHTKMAIKLMLQQDRLSLGRRHVTISTC", "aa_hexdigest": "271c9d5070475482b82cbab1dc6fbc82", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4359.150599999999, "isoelectric_point": 9.18495578765869 }, "id": "NKOCPLAKPK_1574", "locus": "GCA003260355_01543" }, { "type": "cds", "sequence": "contig_1", "start": 831741, "stop": 832061, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAAGCTGGCAATTTCCCTCCATGCTCCTAATCAAGAGTTAAGAAATCAGATTATGCCTATCGGGAAGTCTTATCCTTTAGATAAGCTTATGACAACGATTGATCAGTATGTTGAAGCAACGAACAATAGGATTTTCTATGAATATATTATGATTAAAGATCTAACGGATACTCCTGCATTAGCCCACGAACTCGTCCAGCTTCTCAAAGGCAGACTCGCTCATATTAACCTCATCCCGTATAATGAAAATCCTGCTATTCATCTCCAAGAAAGTAGTAAGCTAGCTATCGCAACCTTCAAGCAGATTCTCGAAAGCTGA", "aa": "MKLAISLHAPNQELRNQIMPIGKSYPLDKLMTTIDQYVEATNNRIFYEYIMIKDLTDTPALAHELVQLLKGRLAHINLIPYNENPAIHLQESSKLAIATFKQILES", "aa_hexdigest": "a98c3d0d0e5cbae85f905d34108b84cb", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12130.996299999988, "isoelectric_point": 6.397933387756347 }, "id": "NKOCPLAKPK_1575", "locus": "GCA003260355_01544" }, { "type": "cds", "sequence": "contig_1", "start": 832071, "stop": 832163, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGACGGTGCGTGATAGCATGGGAAGAGAAGCAAAAGGAGCTTGTGGACAGCTCGGCTATGAGAAAGTGAAGGGACAGCAGTTAGGCTTTTAG", "aa": "MTVRDSMGREAKGACGQLGYEKVKGQQLGF", "aa_hexdigest": "19c2673fa607660d554f6ba08f927527", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3245.710500000001, "isoelectric_point": 9.10056667327881 }, "id": "NKOCPLAKPK_1576", "locus": "GCA003260355_01545" }, { "type": "cds", "sequence": "contig_1", "start": 832349, "stop": 832480, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATGTCTGTAAAAATTGAGCAAATGCATGTAAATGAGCTGCAAAATGTGGAGATAAAGCAAGAAAATATGAAAAAGCAGAAACTACACGAAGACCAATCTCCCAGTTTTGTGGAGAAGGATCTGCCTTAA", "aa": "MMSVKIEQMHVNELQNVEIKQENMKKQKLHEDQSPSFVEKDLP", "aa_hexdigest": "69c70fdfff988f954cb06661fbe73279", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5138.872400000001, "isoelectric_point": 5.5198278427124015 }, "id": "NKOCPLAKPK_1577", "locus": "GCA003260355_01546" }, { "type": "cds", "sequence": "contig_1", "start": 832611, "stop": 832706, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAATGCTGATTACGGAATGATTGTCGGAACTAGTGATGCAAAAAGTAAAGATATTCAGCTTACAATTGGAGGAGCCCTGAGAGTGGGAGCGTGA", "aa": "MNADYGMIVGTSDAKSKDIQLTIGGALRVGA", "aa_hexdigest": "4d4f4dc2f8d064aa9ea9c5ef8943cb20", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3153.5853, "isoelectric_point": 5.794132804870605 }, "id": "NKOCPLAKPK_1578", "locus": "GCA003260355_01547" }, { "type": "cds", "sequence": "contig_1", "start": 832770, "stop": 832925, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGGAATCGAGGCATCTGGTGCGGTTGCAGTGGCTGCGACAAATGTTATTATTAATGCAGAAAACTCTGCCGCTTATGGAGGCTCTAATGTTAAAATCCAACCTGGAGCCTCAGAATCTTTCGTCTTGGGATCAAATGTAGAGATTGCTTCTTGA", "aa": "MGIEASGAVAVAATNVIINAENSAAYGGSNVKIQPGASESFVLGSNVEIAS", "aa_hexdigest": "46e8b7e2699e0f744d65d6e2009c20b4", "start_type": "TTG", "rbs_motif": "TTCTT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4937.407399999999, "isoelectric_point": 4.093055534362793 }, "id": "NKOCPLAKPK_1579", "locus": "GCA003260355_01548" }, { "type": "cds", "sequence": "contig_1", "start": 833600, "stop": 833791, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAGCATGAATATCCGACGGTAAGGCTCTTGGTGAATCTTTCTGGAGATGCAACGGAGTTTTATAGAAAGGATAGTCAGCAAATTTCGGCAAATGCTATGAAGCTACAGGCGGAACTCGCAAAGCTCGACAAAGAGCTCGACTTCGTTTATCAGCAGTTCAAAGGGAAATACCTAACGGGAGCAAAATAA", "aa": "MQHEYPTVRLLVNLSGDATEFYRKDSQQISANAMKLQAELAKLDKELDFVYQQFKGKYLTGAK", "aa_hexdigest": "37562af78575652e4284cfd1ecee6530", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7270.2158, "isoelectric_point": 7.984618949890138 }, "id": "NKOCPLAKPK_1580", "locus": "GCA003260355_01549" }, { "type": "cds", "sequence": "contig_1", "start": 834472, "stop": 834612, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTGATCAAATGAAACAATCTCTTCAGGGGATGAAGCTCTTCCTTTTGGTAATGGGAGCGATTTCTCTGCTTGTTGGAGGAATTGGGGTGATGAATATTATGATCGTTTCCGTAACAGAAAGAACAAGAGAAATCTGA", "aa": "MVDQMKQSLQGMKLFLLVMGAISLLVGGIGVMNIMIVSVTERTREI", "aa_hexdigest": "0c50f032c94c5041baac12e5ee541032", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5052.180399999998, "isoelectric_point": 8.34403018951416 }, "id": "NKOCPLAKPK_1581", "locus": "GCA003260355_01550" }, { "type": "cds", "sequence": "contig_1", "start": 834808, "stop": 835044, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF12704.14" ], "nt": "ATGGTGATGGGGGTTGGTGTTCCAGAACTTCGGTTTACTCTCAATGAAAAGAAAGCAGCTGAAGGAACTCTCTTTTCCAAGGAGCATTACGATAGCAAGAGTTTCGTTGCCGTGGTTAACGAAACGTTCAAGAGCAAGTTCTTCAAAAATGGAAGCGCAATAGGGAAAAAATTCATCTATAACGGTAAAGAGTTTACTATTGTAGGAGTTTTACCGAAACAAGAGTATGACCGATGA", "aa": "MVMGVGVPELRFTLNEKKAAEGTLFSKEHYDSKSFVAVVNETFKSKFFKNGSAIGKKFIYNGKEFTIVGVLPKQEYDR", "aa_hexdigest": "0369846420b264598aa90ecc4ffc0168", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF12704.14", "name": "MacB_PCD", "length": 69, "aa_cov": 0.8846153846153846, "hmm_cov": 0.9565217391304348, "evalue": 2.237228491567157e-11, "score": 40.848323822021484, "start": 5, "stop": 73 } ], "seq_stats": { "molecular_weight": 8828.090099999998, "isoelectric_point": 9.435479927062985 }, "id": "NKOCPLAKPK_1582", "locus": "GCA003260355_01551" }, { "type": "cds", "sequence": "contig_1", "start": 835288, "stop": 835419, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGGTTCTTTTCCTACTTTGTAGATGCTCGAAAGACCATTATGGCCAACAAAATGAGATCTGGCTTGAGTACGCTCGGGATTATCATCGGAATGGCTTCCGTTGTGGTGATGATGGCGATCGGGCAATGA", "aa": "MRFFSYFVDARKTIMANKMRSGLSTLGIIIGMASVVVMMAIGQ", "aa_hexdigest": "362586c21437f4e572764494ca7dd80e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4743.767800000002, "isoelectric_point": 11.000063133239745 }, "id": "NKOCPLAKPK_1583", "locus": "GCA003260355_01552" }, { "type": "cds", "sequence": "contig_1", "start": 835466, "stop": 835975, "strand": "-", "frame": 3, "gene": null, "product": "ABC transporter domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4ILW2" ], "nt": "ATGAATATCGTCGGACTTCTTGATGTTCAGACTTCTGGCTCTTATTTTCTCCGTAAGAAAGAGGTAAAAAATCTGACTCCAGACCAGCAATCGATGATCCGCAGAACAACAATCGGATTCATCTTTCAGGGCTATAATCTGCTCAAAAGAATGTCAGCACGAGAGCAGGTGGTTCTTCCACTTTCGTATATGGGAATTCCTTCAGGAGAAGCTAAGGAGAGGGCCTATGCTGCACTTAAACTCGTAGGACTTGAGGGGAGAGAAAAAGCTCTTCCAAACCAACTTTCTGGAGGTCAACAGCAGAGGGTGTGTATTGCAAGGGCATTAGTGACAGATTCAGGACTTTTGCTCGCAGATGAGCCAACAGGAGCTTTGGATTCCAAGACAGGAATAGAGGTAATGGAGCTTTTTAAAAAATTAAATGCTGAACAGGGAAGAACAATTCTTCTGATTACCCATGACGCAAATGTTGCGAGCTATGCAAATAAAACGATCCGTATCAAAGACTGA", "aa": "MNIVGLLDVQTSGSYFLRKKEVKNLTPDQQSMIRRTTIGFIFQGYNLLKRMSAREQVVLPLSYMGIPSGEAKERAYAALKLVGLEGREKALPNQLSGGQQQRVCIARALVTDSGLLLADEPTGALDSKTGIEVMELFKKLNAEQGRTILLITHDANVASYANKTIRIKD", "aa_hexdigest": "a3d781e7c988689d5c58640c072838ea", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A7W4E403", "query_cov": 1.0, "subject_cov": 0.8407960199004975, "identity": 0.84, "score": 276.0, "evalue": 1.75e-92, "valid": false, "product": "ABC transporter ATP-binding protein", "uniref50_id": "UniRef50_A0A2W4ILW2" }, "pscc": { "uniref50_id": "UniRef50_A0A2W4ILW2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4ILW2" ], "product": "ABC transporter domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_1584", "locus": "GCA003260355_01553" }, { "type": "cds", "sequence": "contig_1", "start": 836003, "stop": 836140, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTAGAAGCAACATCAGATTTTATTTCACTTAAGGGAATCACAAAAACCTATGGAGGAAAGATGACAGAGCCAGTATATGCTCTAAGAGGAGTTGATCTTGATATTCATGATGGAGACTTTATTTCCATTATGTGA", "aa": "MVEATSDFISLKGITKTYGGKMTEPVYALRGVDLDIHDGDFISIM", "aa_hexdigest": "03ba109c16c5cd2c572a3d8384145e78", "start_type": "ATG", "rbs_motif": "TTCTT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4936.633, "isoelectric_point": 4.589998817443848 }, "id": "NKOCPLAKPK_1585", "locus": "GCA003260355_01554" }, { "type": "cds", "sequence": "contig_1", "start": 836242, "stop": 836409, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCGTCTCAAAAAGGGAGAACAGCGAGTTGATCAGGTCGTAGAAGTAGGAATTGCTGATGAAATGAACACGGTAGTTCTTTCTGGTCTTAAGCTTGGTGATACGATAAAGGGATTCTATATGAACGATATTTCTGCAGGGAATGTGGGTGTTGGCAGTATGGGCTGA", "aa": "MRLKKGEQRVDQVVEVGIADEMNTVVLSGLKLGDTIKGFYMNDISAGNVGVGSMG", "aa_hexdigest": "65f2407cecf1938c65276cffe0a2be66", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5828.673100000002, "isoelectric_point": 5.042607688903809 }, "id": "NKOCPLAKPK_1586", "locus": "GCA003260355_01555" }, { "type": "cds", "sequence": "contig_1", "start": 836419, "stop": 836580, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCAGAGGGAAATTCCTACAAAGCTAAAGTAGTTTTCCAAAAAAATAATCCTGAGGAAAAGATTCTAGGAGGAATGAGTGCGACAGTAAAGGTAACCCTTGAAGAAGCAAAAGGAAAGCTAATCGTTCCTAATCCTGCACTTGCAGACAACGAACTCTGA", "aa": "MPEGNSYKAKVVFQKNNPEEKILGGMSATVKVTLEEAKGKLIVPNPALADNEL", "aa_hexdigest": "868f227bd170c2bd067d69ddb441432f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5712.5500999999995, "isoelectric_point": 6.155287742614745 }, "id": "NKOCPLAKPK_1587", "locus": "GCA003260355_01556" }, { "type": "cds", "sequence": "contig_1", "start": 836602, "stop": 836847, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGCAGTATGATGAATACAAAATTATTGCAAATTTCGATGGAAGAGTAACCAAACTTGATATGCAGGTTGGAGACAGTATTGATACAAACTCTACAAATGATAATCAAAAATACATCTATGTAGAGACACCAGATCTTCTTGAGGTAAAAATTGAGGTGGATCAGCTTGATATCGTAAAGATCCAGCAAGGAATGAAAGTCCAGGTTTCTGTTGGTGCTTTCCCAGACCAAGTATATTCATGA", "aa": "MKQYDEYKIIANFDGRVTKLDMQVGDSIDTNSTNDNQKYIYVETPDLLEVKIEVDQLDIVKIQQGMKVQVSVGAFPDQVYS", "aa_hexdigest": "8e7db885512a01a8733434c01f8d8c58", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9277.3425, "isoelectric_point": 4.311544609069825 }, "id": "NKOCPLAKPK_1588", "locus": "GCA003260355_01557" }, { "type": "cds", "sequence": "contig_1", "start": 837280, "stop": 837798, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAAAACAAACCTTGCCAAGTGAACAGCAGCTTGATGATCAGACCAAAAAATTTGATCTTGAAGAAGCAGAAAGGCAAATTAGAGAAAAGGAGAAAGACCTCAAAGAAGCCCAGACTGATTATGCTCAGCTCCTATCAGGAAAGGCAGGAGCAACGAATGCAGACTTAGCAGTTTCCAAGAACGTAAGAACCAGAAACACTTCTATGGAAAAATTGGTGAGAGCATTTCGTGAGGAAGCAAATGCTTTGCAATCTACGCTAGATAATTTTGATGCGGTGATGAAACTAACTGACCAATACAAAGGAGAAGAAATGAATACCTATATTGGAGCAAAAAATACGGCATTAATTGCTCAGTCAAAAGAGCAGTTCCGAAAAGTCTATGCATATAAGGCAAGACTTAATGATCTTTATACTGAATTTTCAAAGATTCCGGTTGGTCAGCTGACTGAAAACAAGATTCTTTCAGGATATACTGTTTTCAAAGAGTTAGGTGCTGATCTAAGGAATCGATGA", "aa": "MQKQTLPSEQQLDDQTKKFDLEEAERQIREKEKDLKEAQTDYAQLLSGKAGATNADLAVSKNVRTRNTSMEKLVRAFREEANALQSTLDNFDAVMKLTDQYKGEEMNTYIGAKNTALIAQSKEQFRKVYAYKARLNDLYTEFSKIPVGQLTENKILSGYTVFKELGADLRNR", "aa_hexdigest": "1a51943c664df3cd041c04d3d34f7690", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19684.928600000003, "isoelectric_point": 7.782446098327637 }, "id": "NKOCPLAKPK_1589", "locus": "GCA003260355_01558" }, { "type": "cds", "sequence": "contig_1", "start": 838719, "stop": 838979, "strand": "+", "frame": 3, "gene": null, "product": "(d)CMP kinase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A288DLL8", "UniRef:UniRef90_A0A328EL13" ], "nt": "TTGGATGTTCGTGATGAGGTCGCAAGTGAAAGAATCCGCAAAGATCAGAGAACTACCGATCAATTCTCTTCAAAAGATGAGGCTTTATCTGCGACAAAAAAAAGGAATGCTGACGATAGAGCCAGATTCCTTTCTCTTTATGAAGTTGATCTCTGGGATGATAGGAATTATGATCTCAAAATAGATACAAGCGACCTCTCTGCTGAACAAGTTTGCTCTAGGATTATCGCCAGATTTAGCGAAATAGTCCCTATTAAATAA", "aa": "MDVRDEVASERIRKDQRTTDQFSSKDEALSATKKRNADDRARFLSLYEVDLWDDRNYDLKIDTSDLSAEQVCSRIIARFSEIVPIK", "aa_hexdigest": "cf267d0bc879ef4f3c0715831d1f781a", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 838428, "stop": 838979, "observations": { "mutation": [ 838443, 838521, 838584 ], "stop": [ 838443, 838521, 838584 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EL13", "query_cov": 1.0, "subject_cov": 0.46994535519125685, "identity": 0.965, "score": 159.0, "evalue": 6.05e-48, "gene_start": 1, "gene_end": 86, "reference_start": 98, "reference_end": 183, "reference_sequence": "MIITLGGKAGSGKGTVSKLLAQELDYEIISIGTLKRKIAEEMGLSISEFNLLGEKAENQREFDLKYEEYQKSLPLESRIILESRLGFLCQPKAFKVFLDVRDEVASERIRKDQRTTDQFSSKDEALSATKKRNADDRARFLSLYEVDLWDDRNYDLKIDTSDLSAEQVCSRIIARFSEIVAVK" }, "paralog": false, "identity": 0.9726775956284153, "score": 339.0, "evalue": 1.56e-121, "description": "Internal stop codon at 838443, 838521, 838584. Nonsense mutation around 838443, 838521, 838584.", "psc": { "uniref90_id": "UniRef90_A0A328EL13", "product": "(d)CMP kinase", "uniref50_id": "UniRef50_A0A288DLL8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EL13", "UniRef:UniRef50_A0A288DLL8" ] }, "pscc": { "uniref50_id": "UniRef50_A0A288DLL8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A288DLL8" ], "product": "(d)CMP kinase" } }, "id": "NKOCPLAKPK_1590", "locus": "GCA003260355_01559" }, { "type": "cds", "sequence": "contig_1", "start": 839033, "stop": 839404, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02637.23" ], "nt": "ATGACGGCACATTTTGTGAATATTACCGAGCTTCCTATCGACCAAGAGCAGTTGATTGAGTTTTTCAACATTGCAAAAGAAGGAAAACTGATTGACAATCAACTCAAACTCATCATGGAAGAAATGCTCCAAACGGGAGCTTCAGCATCTGATATCATCAAGGAAAAAGGCTTTGATAGTCCAGAGATCTCAGAATCTGATTTAAGAGCTTTTTGTAAAGAGGTTATAGACGAGAATCCAGCAATCCTTGATCAGTATAAAGCAGGGAAAACCAGTGTTATAGGATTTTTTGTAGGGCAAGTAATGAGGAAAACGCAGGGAAAAGCGAATCCTCAAGCAATCACCCAAATCCTACTCTCAGAATTAGGCTAA", "aa": "MTAHFVNITELPIDQEQLIEFFNIAKEGKLIDNQLKLIMEEMLQTGASASDIIKEKGFDSPEISESDLRAFCKEVIDENPAILDQYKAGKTSVIGFFVGQVMRKTQGKANPQAITQILLSELG", "aa_hexdigest": "bd3d38a3d81893a86a276ad794ead99d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02637.23", "name": "GatB_Yqey", "length": 117, "aa_cov": 0.9512195121951219, "hmm_cov": 0.9914529914529915, "evalue": 6.758646878104153e-34, "score": 113.34097290039062, "start": 6, "stop": 122 } ], "seq_stats": { "molecular_weight": 13674.553099999985, "isoelectric_point": 4.6576940536499025 }, "id": "NKOCPLAKPK_1591", "locus": "GCA003260355_01560" }, { "type": "cds", "sequence": "contig_1", "start": 839534, "stop": 839686, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02934.20" ], "nt": "ATGAGAAGTAAAGAAGACGCGCTTGATTACAGATATTTCCCAGAGCCAGATTTGCTTCCGCTTTATCTTGATGAGCAGCTTCTTAAAAAGGTGGAGGAAGCTGAGTTGATTATCCCTTTTCAAGAAATTAAAAAAATGAAGTCAGAATACTGA", "aa": "MRSKEDALDYRYFPEPDLLPLYLDEQLLKKVEEAELIIPFQEIKKMKSEY", "aa_hexdigest": "e0568ee783cae183cabeed9128a6a574", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02934.20", "name": "GatB_N", "length": 28, "aa_cov": 0.56, "hmm_cov": 1.0, "evalue": 1.1305690671646258e-08, "score": 30.991859436035156, "start": 1, "stop": 28 } ], "seq_stats": { "molecular_weight": 6107.008699999998, "isoelectric_point": 4.644507408142091 }, "id": "NKOCPLAKPK_1592", "locus": "GCA003260355_01561" }, { "type": "cds", "sequence": "contig_1", "start": 839699, "stop": 840322, "strand": "-", "frame": 3, "gene": "gatB", "product": "Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB", "db_xrefs": [ "EC:6.3.5.6", "EC:6.3.5.7", "GO:0016884", "KEGG:K02434", "SO:0001217", "UniRef:UniRef50_A0A288GU77", "UniRef:UniRef90_A0A660MZK5" ], "nt": "TTGCCTCAGCTCAGTGCAGAGGTCGTACAGAAGGCACTTCTGCTAGGAAAGGCTCTGCAATGCCAATTCAATAATCCTTCATATTTTGATAGAAAATCCTATTTCTATCCAGATTTGCCAATGGGATACCAAATTACTCAGCTCTATACGCCAATTAACGTAAAAGGAACACTTTCTTTTTTCTTTGCAAATTATGAAGAGGAGAAACAGGTAAGAATCTCAGACGCCCATCTAGAATGTGATACTGCGAAGGCTTTTCATCAGGGAGGAGCAATGTTGCTAGACTTTAACCGTGCCGGGACTCCGTTGATTGAAATCGTGACGGGACCTGATTTTACCAGCGCAGAACAAGCTGTAGAGTTTGCCAAAGAAATCCAAAGAATTGCAAAACGAAATAACCTTTCAGATGCAGATATGGAAAGGGGGCAAATGAGAGTCGATGTCAATGTTTCCATTAGAAAAGAAAAATCTGATCCTCTAGGAACGAGGGTAGAACTCAAAAATATCAACTCCTTTTCCGCTATAAAAAGAGCAATAGATGCTGAAGTGATAAGACAGGCAAAATGCCGAGAAACAGGAGAAGAAATTCAGCAGCAGACGAGGAGACGAGATGATCTTAAGTGA", "aa": "MPQLSAEVVQKALLLGKALQCQFNNPSYFDRKSYFYPDLPMGYQITQLYTPINVKGTLSFFFANYEEEKQVRISDAHLECDTAKAFHQGGAMLLDFNRAGTPLIEIVTGPDFTSAEQAVEFAKEIQRIAKRNNLSDADMERGQMRVDVNVSIRKEKSDPLGTRVELKNINSFSAIKRAIDAEVIRQAKCRETGEEIQQQTRRRDDLK", "aa_hexdigest": "27824b90521faea1f6b6fa25b9f9ff8d", "start_type": "TTG", "rbs_motif": null, "genes": [ "gatB" ], "truncated": "both", "pseudogene": { "start": 839396, "stop": 840475, "observations": { "mutation": [ 839536, 839701, 840328, 840337 ], "stop": [ 839536, 839701, 840328, 840337 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A660MZK5", "query_cov": 1.0, "subject_cov": 0.43125, "identity": 0.971, "score": 394.0, "evalue": 1.95e-134, "gene_start": 1, "gene_end": 207, "reference_start": 52, "reference_end": 258, "reference_sequence": "MQEPLITIGLEIHLKLASQTKIFCKCKNEQALQDNQPNTTICPVCTGQPGALPQLSAEVVEKGLLLGKALQCQFNNPSYFDRKSYFYPDLPMGYQITQLYTPINVKGALSFFFANYEEEKQVRISDAHLECDTAKAFHQGGVMLLDFNRAGTPLIEIVTGPDFTSAEQAVEFAKEIQRIAKRNNLSDADMERGQMRVDVNISIRKEKSDPLGTRVELKNINSFSAIKRAIDAEVIRQAKCRETGEEIQQQTRRRDDLKGQSFAMRSKEDALDYRYFPEPDLLPLYLDEQLLKKVEEAELIIPFQEIKKMKSEYGFHKEFINALISEYPTLTFFKQLIHAGFEPKLVAKRIAGQISGYMTAHFVNITELPVDQEQLIEFFNIAKEGKLIDNQLKLIMEEMLQTGASASDIIKEKGFDTPEISESDLRAFCKEVLHENPAILDQYKAGKTSVIGFFVGQVMRKTQGKANPQAISKILLSELG" }, "paralog": false, "identity": 0.9721448467966574, "score": 691.0, "evalue": 6.13e-254, "description": "Internal stop codon at 839536, 839701, 840328, 840337. Nonsense mutation around 839536, 839701, 840328, 840337.", "psc": { "uniref90_id": "UniRef90_A0A660MZK5", "gene": "gatB", "product": "Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB", "ec_ids": [ "6.3.5.6", "6.3.5.7" ], "uniref50_id": "UniRef50_A0A288GU77", "kegg_orthology_id": "K02434", "go_ids": [ "GO:0016884" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A660MZK5", "EC:6.3.5.6", "EC:6.3.5.7", "UniRef:UniRef50_A0A288GU77", "KEGG:K02434", "GO:0016884" ] }, "pscc": { "uniref50_id": "UniRef50_A0A288GU77", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A288GU77" ], "product": "Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B" } }, "id": "NKOCPLAKPK_1593", "locus": "GCA003260355_01562" }, { "type": "cds", "sequence": "contig_1", "start": 840335, "stop": 840634, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02934.20" ], "nt": "ATGATAAAAAATTTATACCATAGACACTCTATCTATACTGTTGTGGAGAGCATTTTCAAGTCAGTGTCTTCTTATATCCCAGATTTTTTAAAAAATCTTTTGTTTTCGGTATTTAAATTGCTATACTCTCAGTCAGTTTTCATTTCTTTCCTCCTAAATATGCAAGAACCGCTCATTACTATTGGACTAGAGATTCACTTAAAGCTCGCAAGTCAGACCAAAATTTTCTGTAAATGTAAAAATGAACAAGCCCTTCAAGATAACCAGCCCAACACCACTATTTGTCCGGTATGTACTTGA", "aa": "MIKNLYHRHSIYTVVESIFKSVSSYIPDFLKNLLFSVFKLLYSQSVFISFLLNMQEPLITIGLEIHLKLASQTKIFCKCKNEQALQDNQPNTTICPVCT", "aa_hexdigest": "0d8a9bec53567c0adf3c571053013b76", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02934.20", "name": "GatB_N", "length": 40, "aa_cov": 0.40404040404040403, "hmm_cov": 0.925, "evalue": 7.871377091090666e-11, "score": 38.06810760498047, "start": 60, "stop": 99 } ], "seq_stats": { "molecular_weight": 11431.366999999984, "isoelectric_point": 8.734063911437985 }, "id": "NKOCPLAKPK_1594", "locus": "GCA003260355_01563" }, { "type": "cds", "sequence": "contig_1", "start": 840826, "stop": 840996, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTGATCAAAGCACAACCCATTATTGATACTGTAGAATTTGTGATCAAGGATAACCAGATAAAAAAATCAGATTTTTCTATTCTTTTTCCAACGCCAACCGAGCAGCTCTTTAACCAAAAAATCGCTCATGGACTCAGTAAAAAAGAGCATCTCATTTTCATCTGCTGA", "aa": "MLIKAQPIIDTVEFVIKDNQIKKSDFSILFPTPTEQLFNQKIAHGLSKKEHLIFIC", "aa_hexdigest": "95cccfe2ed73ee6a9f999f28d13c468b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6470.598799999998, "isoelectric_point": 7.914670753479004 }, "id": "NKOCPLAKPK_1595", "locus": "GCA003260355_01564" }, { "type": "cds", "sequence": "contig_1", "start": 841117, "stop": 841335, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTGAGGCTATTACTAGACTAATTCCTGGAGTTATAAAAGAAAAAGGAAGCTGGATTCATGAGAGCTATTCTCCTAGAGATAATATGAGCAATATTGAAGCTCCCAATTATACAAGACCGATTGAAGTCTATGGGATGAAGGTCCCAGAAGTCTTACTCAATGGCGATGAGGAGGAAATCGCTCAGTGGAGAGAAGAACATAGTATTAACCTCTAA", "aa": "MIEAITRLIPGVIKEKGSWIHESYSPRDNMSNIEAPNYTRPIEVYGMKVPEVLLNGDEEEIAQWREEHSINL", "aa_hexdigest": "5434e96d8b22345024b5b3d09eccd496", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8338.333999999999, "isoelectric_point": 4.742156791687012 }, "id": "NKOCPLAKPK_1596", "locus": "GCA003260355_01565" }, { "type": "tRNA", "sequence": "contig_1", "start": 841802, "stop": 841878, "strand": "-", "gene": "trnG", "product": "tRNA-Gly(tcc)", "amino_acid": "Gly", "anti_codon": "tcc", "score": 81.4, "nt": "GCGGGTGTAACTCAATTGGCTAGAGTGCCTGCCTTCCAAGCAGACTGTTGCGGGTTCGAGTCCCGTCACCCGCTCCA", "db_xrefs": [ "SO:0000260" ], "anti_codon_pos": [ 841842, 841844 ], "id": "NKOCPLAKPK_25", "locus": "GCA003260355_01566" }, { "type": "tRNA", "sequence": "contig_1", "start": 841938, "stop": 842011, "strand": "-", "gene": "trnI", "product": "tRNA-Ile2(cat)", "amino_acid": "Ile2", "anti_codon": "cat", "score": 80.3, "nt": "GGGCCTATAGCTCAGTTGGTTAGAGCAGAAGACTCATAATCTTTTGGTCGCTGGTTCAAGTCCAGCTAGGCCCA", "db_xrefs": [ "SO:0000263" ], "id": "NKOCPLAKPK_24", "locus": "GCA003260355_01567" }, { "type": "tRNA", "sequence": "contig_1", "start": 842053, "stop": 842129, "strand": "-", "gene": "trnV", "product": "tRNA-Val(tac)", "amino_acid": "Val", "anti_codon": "tac", "score": 86.9, "nt": "GGGTGATTAGCTCAGTTGGCTAGAGCATCTGCCTTACAAGCAGGGGGTCATAGGTTCGAGTCCTATATCACCCACCA", "db_xrefs": [ "SO:0000273" ], "anti_codon_pos": [ 842093, 842095 ], "id": "NKOCPLAKPK_23", "locus": "GCA003260355_01568" }, { "type": "tRNA", "sequence": "contig_1", "start": 842137, "stop": 842208, "strand": "-", "gene": "trnE", "product": "tRNA-Glu(ttc)", "amino_acid": "Glu", "anti_codon": "ttc", "score": 50.9, "nt": "GGTCTTATCGTCTAACGGCTAGGACAGGAGACTTTCAATCTTCTAATCGGGGTTCGATTCCCCGTAAGACCG", "db_xrefs": [ "SO:0000259" ], "anti_codon_pos": [ 842173, 842175 ], "id": "NKOCPLAKPK_22", "locus": "GCA003260355_01569" }, { "type": "tRNA", "sequence": "contig_1", "start": 842222, "stop": 842298, "strand": "-", "gene": "trnD", "product": "tRNA-Asp(gtc)", "amino_acid": "Asp", "anti_codon": "gtc", "score": 86.6, "nt": "GGGGTGGTAGTTCAGCTGGTTAGAACGCCTGCCTGTCACGCAGGAGGTCGAGGGTTCGAGTCCCTTCCATCCCGCCA", "db_xrefs": [ "SO:0000256" ], "anti_codon_pos": [ 842262, 842264 ], "id": "NKOCPLAKPK_21", "locus": "GCA003260355_01570" }, { "type": "tRNA", "sequence": "contig_1", "start": 842332, "stop": 842407, "strand": "-", "gene": "trnT", "product": "tRNA-Thr(tgt)", "amino_acid": "Thr", "anti_codon": "tgt", "score": 81.0, "nt": "GCCAGCTTAGCTCAATAGGTAGAGCAACCGCCTTGTAAGCGGTAGGTTGTCGGTTCAATTCCGACAGCTGGCTCCA", "db_xrefs": [ "SO:0000270" ], "anti_codon_pos": [ 842372, 842374 ], "id": "NKOCPLAKPK_20", "locus": "GCA003260355_01571" }, { "type": "cds", "sequence": "contig_1", "start": 842427, "stop": 842690, "strand": "-", "frame": 2, "gene": "rpsT", "product": "30S ribosomal protein S20", "db_xrefs": [ "GO:0003723", "GO:0003735", "GO:0005840", "GO:0006412", "KEGG:K02968", "SO:0001217", "UniParc:UPI000DBF7A3B", "UniRef:UniRef100_A0A328EL32", "UniRef:UniRef50_A0A2M7XJ90", "UniRef:UniRef90_A0A328EL32" ], "nt": "ATGCCAATCAAGAAGTCCGCGGTTAAAGCTATGAATAGATCTCAAACGCTTGCAAAGAGGAATTACGATTTCAAGTTGAGGATGAGGATGGCAATCAAAAAATTCCTTAAAGGAGTTGAGGCAAAAGAATCTTTGACTGTTGAGGATTTGAGTAAAGTATACAAAGCTATTGATAAAGCCGCTAAGGTGGGAATCCTTAAGAAGAGGAACGCCGCTAGAAAAAAGGCTAGAGTTTCAAAAGCTTTTGATACTCTCAAGAAATAG", "aa": "MPIKKSAVKAMNRSQTLAKRNYDFKLRMRMAIKKFLKGVEAKESLTVEDLSKVYKAIDKAAKVGILKKRNAARKKARVSKAFDTLKK", "aa_hexdigest": "fa5c5fd41b85e5ff8bef15c59b07a503", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000DBF7A3B", "uniref100_id": "UniRef100_A0A328EL32", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBF7A3B", "UniRef:UniRef100_A0A328EL32" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EL32", "uniref90_id": "UniRef90_A0A328EL32" }, "psc": { "uniref90_id": "UniRef90_A0A328EL32", "gene": "rpsT", "product": "30S ribosomal protein S20", "uniref50_id": "UniRef50_A0A2M7XJ90", "kegg_orthology_id": "K02968", "go_ids": [ "GO:0003723", "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_A0A2M7XJ90", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2M7XJ90" ], "product": "Small ribosomal subunit protein bS20" }, "genes": [ "rpsT" ], "id": "NKOCPLAKPK_1597", "locus": "GCA003260355_01572" }, { "type": "tRNA", "sequence": "contig_1", "start": 842724, "stop": 842795, "strand": "-", "gene": "trnN", "product": "tRNA-Asn(gtt)", "amino_acid": "Asn", "anti_codon": "gtt", "score": 74.3, "nt": "TCCGAGGTAGCTCAGCGGTAGAGCAGATGGCTGTTAACCATTTGGTCGTGGGTTCGATCCCCACCCTCGGAG", "db_xrefs": [ "SO:0000257" ], "anti_codon_pos": [ 842761, 842763 ], "id": "NKOCPLAKPK_19", "locus": "GCA003260355_01573" }, { "type": "tRNA", "sequence": "contig_1", "start": 842939, "stop": 843020, "strand": "-", "gene": "trnL", "product": "tRNA-Leu(tag)", "amino_acid": "Leu", "anti_codon": "tag", "score": 63.3, "nt": "GCCGGGATGGTGGAACTGGTAGACACGCTTGGCTTAGAACCAAGTGCCTTACGGTGTGCAGGTTCAAGTCCTGTTCCCGGTA", "db_xrefs": [ "SO:0000264" ], "anti_codon_pos": [ 842999, 843001 ], "id": "NKOCPLAKPK_18", "locus": "GCA003260355_01574" }, { "type": "tRNA", "sequence": "contig_1", "start": 843037, "stop": 843109, "strand": "-", "gene": "trnQ", "product": "tRNA-Gln(ctg)", "amino_acid": "Gln", "anti_codon": "ctg", "score": 73.0, "nt": "TCGGGAGTAGCTTAATTGGTAGAGCATCAGACTCTGAATCTGGGGGTTATAGGTTCGAGTCCTATCTCCCGAG", "db_xrefs": [ "SO:0000261" ], "anti_codon_pos": [ 843074, 843076 ], "id": "NKOCPLAKPK_17", "locus": "GCA003260355_01575" }, { "type": "tRNA", "sequence": "contig_1", "start": 843116, "stop": 843190, "strand": "-", "gene": "trnfM", "product": "tRNA-fMet(cat)", "amino_acid": "fMet", "anti_codon": "cat", "score": 65.3, "nt": "TGCGGAGTAGAGAAACGGTATCTCGTCGGGCTCATAACCCGAAGATAGGTGGTTCAATTCCATCCTCCGCAACCA", "db_xrefs": [ "SO:0000266" ], "id": "NKOCPLAKPK_16", "locus": "GCA003260355_01576" }, { "type": "tRNA", "sequence": "contig_1", "start": 843220, "stop": 843290, "strand": "-", "gene": "trnQ", "product": "tRNA-Gln(ttg)", "amino_acid": "Gln", "anti_codon": "ttg", "score": 55.0, "nt": "TGGGGAATCGTCTAAAGGTAGGACACCGGACTTTGACTCCGTTTGTCCAGGTTCGAATCCTGGTTCCCCAG", "db_xrefs": [ "SO:0000261" ], "anti_codon_pos": [ 843256, 843258 ], "id": "NKOCPLAKPK_15", "locus": "GCA003260355_01577" }, { "type": "cds", "sequence": "contig_1", "start": 843492, "stop": 844619, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAGGTTGGTTCGGAGGAAGTGAGCTCGTTAATAAAGGACTCGTAAAGAATGTCTTTGATTCACTCAATAATCAGGAAGATTATCCAATCTTACTTCCATTATCTTCCAAGTTGCTGATCAAAGAATTTGGAAAAACTACCTCAATAACTGATAAGAAGAATACTACACTTACGTGGATGCCAAACGGTGGTCTCCTTGGAGAATACAAGCTTGCCTTAGAAGATGTAGGATACAAGAGATGTACTACTGACAAAGATGGTAAAGGAACCGCTGGTAATTTGAGATGGGAAGGGGAAATCATCTGTGAAGTAGACTTCGCAGTAACAGAGCCTTATCTTATCCAAAAGAGTCCATATGGAATTGGAAATAAAGCTACGACAGACCTTACGAAATACCAGTTGAAAAACGGAACTAACTTTATGGGTGAGTTCTTCAAGACGACTGAGATTTCGAATAAGGCGTATGAAACACCAAAGAATATGAAGAATCTCTTCACTCAGTTCAAAAATAAGTACCAAAAGTTAGCAAAGTCTCTTGGAAATAACGGACTATCAAAGGTTCCAGGTAAGTCAATCTACTTTTATGATGGAACTGAAGAGCTTACAAGCTTACTTGGTGCAAAAATAACCAATAAACCATTTACACTGATCGCTTCAAAGGGACAAAACCTTACGATCAAAGGTAATCTTCCTACAAATGCAATGATTATGACGGAGGGAACCATTACTTTTGATGCAGGTGGTTCTTGTAATGGAGATCGCAAGACCTATGGACACGCAGGACAGATGGTGCAAGGAATCTTCTATGCAGGACAAGGATTTAAATCATCTAATGATCAGGATCTCAAAAATACCTTTGGAAACCTCAGAAAAGGTGAACGATGTAACTATGGAAACCTTCATATCAAGGGAGTTGCAATCGGAGACCTTTCAGATGTTGTTGAGAAGAGAAGATCTGAACTCTATACATGGTTTAGTGCAGATTCTAGCAAAGGATCAGAAAAGAAAAAGGATATTATTGTGAATGGAGCGTCAGTTCTCGTAGAATACAACCCAAGTTTGCGAGGTGCTCTTCCTCCAGGTGCAGAAGAGTTCAATAAAGCACTTGAAGTCTATAGAAAATAA", "aa": "MQGWFGGSELVNKGLVKNVFDSLNNQEDYPILLPLSSKLLIKEFGKTTSITDKKNTTLTWMPNGGLLGEYKLALEDVGYKRCTTDKDGKGTAGNLRWEGEIICEVDFAVTEPYLIQKSPYGIGNKATTDLTKYQLKNGTNFMGEFFKTTEISNKAYETPKNMKNLFTQFKNKYQKLAKSLGNNGLSKVPGKSIYFYDGTEELTSLLGAKITNKPFTLIASKGQNLTIKGNLPTNAMIMTEGTITFDAGGSCNGDRKTYGHAGQMVQGIFYAGQGFKSSNDQDLKNTFGNLRKGERCNYGNLHIKGVAIGDLSDVVEKRRSELYTWFSADSSKGSEKKKDIIVNGASVLVEYNPSLRGALPPGAEEFNKALEVYRK", "aa_hexdigest": "0ce99c7698f52e65d6d4fbd7121aa166", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 41373.51000000001, "isoelectric_point": 9.125322532653808 }, "id": "NKOCPLAKPK_1598", "locus": "GCA003260355_01578" }, { "type": "cds", "sequence": "contig_1", "start": 844647, "stop": 844913, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGGGAGAAGCGCTTCCATTCTACTGGAATATGCAAGCTCAGCTTGGACTCAAAACTGTCTCAGAAAAGCAGAATTGGTTGAAGACAAATTTTGCTTACTCATGTTCAGATCGTGATGCAAATAGCAAGATCGAACTTAGCTCAATGCAATGTAAGTTTGCAGTCTATGCACCAGGAAAAGGAAATGGTAGAAAGGTATATGAGTTTACTACAGATTGTGTAGATATTAACGGATGGGATACTGCATTAGGAAATCAATACTGA", "aa": "MKGEALPFYWNMQAQLGLKTVSEKQNWLKTNFAYSCSDRDANSKIELSSMQCKFAVYAPGKGNGRKVYEFTTDCVDINGWDTALGNQY", "aa_hexdigest": "d284091c86678202fee0856a10477573", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9974.132799999996, "isoelectric_point": 7.650778388977051 }, "id": "NKOCPLAKPK_1599", "locus": "GCA003260355_01579" }, { "type": "cds", "sequence": "contig_1", "start": 844976, "stop": 845248, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTGGAGAAGGACATGATGAAGACCCAGCCAGAGTCTACAAGACTACCTCAGGATGAGGAGGTTGAGGATGAGGTGGTAGTCGAGGATGAGGAGGTTGAGGATGAGGAGGGCGATATGCTCCATCCTGTGGAAATGGTAGAAAAGACGGAAACGAAGTCTGTGATCTTGGAGGACATGGAACCATCGGATCTGATGGAAGACTCTTCAGGAATACGGACGAGTATGATACTAGATATGCAGGATACACTTGTACCAATACGTGTAGACTAG", "aa": "MVEKDMMKTQPESTRLPQDEEVEDEVVVEDEEVEDEEGDMLHPVEMVEKTETKSVILEDMEPSDLMEDSSGIRTSMILDMQDTLVPIRVD", "aa_hexdigest": "3968095d7d96b5205a1c4b86693bda59", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10357.436099999992, "isoelectric_point": 4.0500284194946286 }, "id": "NKOCPLAKPK_1600", "locus": "GCA003260355_01580" }, { "type": "cds", "sequence": "contig_1", "start": 845190, "stop": 845978, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAGGTCGTAGATACCTTGCCAAAAGGACTTGTATTCGTTCCGAATACTCAGTTCCTTACAGGACAAACTACTACAACAGAGCTCGTAAGCTTTGCTACGGGTGTAAATGGAACAGGAGAAGCAACACTCAATTGGGAACTGAAATTCCCACAAGGATTCAAAAAATCAGATTCTTTCCTATTAAAACTTAAGGCAAGATTGGTAGAATCCTCAAGGACGAAATACACCAATATGGCATGTGTTTTCAACCCTGATGATCCAAAGGATCCAAAGTGTGATCCAGAGGACGTTACTCCAAAGCTAGAAGATGTAAAGCTTAAGATCAAAAAATACGTCAGCACCTCAGTAGGTGGCTCACGAGAAGATAATCAGCTTAATATGGACAATGGGCCAGCTTACTTTAAGCTTGTAATATCCAATGCAACTGCACCTTTGACTGGATTCAGGGTAACAGATAGAATAGAAGGAAATCTGACTTTTGATACAGGTTCTTGGGTTCTAGCAGATAATGCTTTTACTGGAGTGATTACTTTTGGACCAAATAATCCAAGTAAGCCAGTATATAAGATTATTCCTACAGTTTCAGGAACTCAGCCTGCAGATATTGCATGGAAGGTAGATATGGGAACAGGGTACTTTATGTCAGGTGATTCCCTCACTATTATCATCAAGACTCAGAAGAAAGGTGACCAAAACAATATCGGTCATGTGTATTATCCAAAACCAAATGGTGGAGAAGGACATGATGAAGACCCAGCCAGAGTCTACAAGACTACCTCAGGATGA", "aa": "MKVVDTLPKGLVFVPNTQFLTGQTTTTELVSFATGVNGTGEATLNWELKFPQGFKKSDSFLLKLKARLVESSRTKYTNMACVFNPDDPKDPKCDPEDVTPKLEDVKLKIKKYVSTSVGGSREDNQLNMDNGPAYFKLVISNATAPLTGFRVTDRIEGNLTFDTGSWVLADNAFTGVITFGPNNPSKPVYKIIPTVSGTQPADIAWKVDMGTGYFMSGDSLTIIIKTQKKGDQNNIGHVYYPKPNGGEGHDEDPARVYKTTSG", "aa_hexdigest": "bf3b3357b0e988e75aed668da96d28a2", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 28616.060099999995, "isoelectric_point": 7.657996940612794 }, "id": "NKOCPLAKPK_1601", "locus": "GCA003260355_01581" }, { "type": "cds", "sequence": "contig_1", "start": 845979, "stop": 846587, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01345.24" ], "nt": "TTGTGTACTACAGGAACCTACGGAAACTTTAACTATGACCAAGCTACTCAAAAATGGACTTGGACATGTAATGGAGAAGCAGGATCCACGCCTGTGCAATGTAGTGCAGTAAAGCCGAGCCGCCCAAATATCGAAAAAGATCTCATTGGAGATAAAAATCATAAATACAAAGTGGGAGAATTGGTTGGCTTCAGAATGCCATTTGGAAATACTGGAAGTCAGACTATTAATAACGTAAAGATTAGAGATATTCTGCCTCTAAACCTTGAATACGTAAGTAGTGCAATCCAAGGAGTGCCTTCTTATACAAACGGAGTTTATCTGAGTGGAGGAGTACAAGTCCTAGAGTATTCAGGATTTAGCCTCAGTTCAGGGCAACAGGGAGTGCTCCTTATGACAGGTAGAGTGCTTAATACTAACCCAGACAATAGGACAAACCGAGTAGGAATCTATGAAAATAACTATCTTTGGGATTATGATACTGAACCGTATCAGCCTGCTCCAAGAGCTCTTCATATCGAAAAAACTGTTGATAAACCACAGGTTTTCAGCGGAGAGATCGTAGAGTTTACGATTAAGGTTTCAAATACTGAAGGAACATTTACTTGA", "aa": "MCTTGTYGNFNYDQATQKWTWTCNGEAGSTPVQCSAVKPSRPNIEKDLIGDKNHKYKVGELVGFRMPFGNTGSQTINNVKIRDILPLNLEYVSSAIQGVPSYTNGVYLSGGVQVLEYSGFSLSSGQQGVLLMTGRVLNTNPDNRTNRVGIYENNYLWDYDTEPYQPAPRALHIEKTVDKPQVFSGEIVEFTIKVSNTEGTFT", "aa_hexdigest": "796f936003a697d4c34a079d980425ee", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01345.24", "name": "DUF11", "length": 103, "aa_cov": 0.504950495049505, "hmm_cov": 0.883495145631068, "evalue": 6.30138770282195e-15, "score": 51.983760833740234, "start": 53, "stop": 154 } ], "seq_stats": { "molecular_weight": 22379.743100000018, "isoelectric_point": 5.71035213470459 }, "id": "NKOCPLAKPK_1602", "locus": "GCA003260355_01582" }, { "type": "cds", "sequence": "contig_1", "start": 846693, "stop": 847874, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTAAAGTACACCGTTACTTACTCCAAGTCTCCGGACAAGAAGGCTCCTAGGACCTCTCATGTAGAGTGTAAATACTACGAAATTACCCGATGTGGAGATGGAATAGTAGATACAGATAGAGGAGAGATCTGTGATCCAAAAGACCCAAATAAAACTGGTTGGGGAAATGGAGGTTGTGATGCTTCATGTAAGCCGATCAATACCGTGGTAGAACAGCCAAAATGTAATAGTGATTACAATGGAAAAACTATCTATAATGAGAAACATCCAGTAGCTGCTCTTAATAGCGATATGGCACTTTGTAGCAAGGGAAAGGTGACATCTTTTGTAGGACCAGATCAAAATGGAAATTATTCATGGACATGTAATAATGCCTCATCCAACGTCAGTTGTAGCGCAAAGGAAATGCGATGTGGCGATGGAGCCAAAAACGGTTCAGAGCAATGTGATCCAAAAGATCCAAATAAAACTGGTTGGGGAAATGGAGGTTGTGATGCTTCCTGTAAGCCAATCAATACCATAATTCCAAAGGCTGACTGTGATGCTACTTTCTATGGCAAGTTAAGACATGGTCGTGGATACGTCTTTGATGATCAAATAACCGGGACACCAACAAAAAGTCGATATTTAAAAGATTTCAAGGTAGATCATAGGGAACAGCTTGATTTTAATGGAGCAGATCCATTCCCAAGCTTTGAATGGACGAAAGAGATTAAAAATCAGAATATGATCCTTAATCCAAATCAAACCATAAAAATCTTGACTGCTACTTCTCCATATTACGTGAGAGAAAAGCCTATAAAAAGAGACAAAAATAATCTGTATCTAGAGTATACTATTTGGTATTCAGATAGTCAAAATGGAGCCTGGTCTGCAGAAAAAGAGTGTAAATATTACGAAATTACTCGATGTGGAGACGGAATTCTTGATCCTGAATATGGAGAAACTTGTGATCCAAATGATCCTAGTAAAACCGGTTGGGGAAAAGGTGGATGTGATGAAAACTGTAAACCCAAGGACATTCCTCAAATCCCAGGACAGTGTAATAGTCAATACAACGGACAAACAGTAGAAAATCTGACCGGAGGAAATCACTTGTGTACTACAGGAACAATGACACAATTCCAGTTCAACTCTGCAACCAATAGATGGACTTGGAAATGTGACGGAATGAACTGA", "aa": "MVKYTVTYSKSPDKKAPRTSHVECKYYEITRCGDGIVDTDRGEICDPKDPNKTGWGNGGCDASCKPINTVVEQPKCNSDYNGKTIYNEKHPVAALNSDMALCSKGKVTSFVGPDQNGNYSWTCNNASSNVSCSAKEMRCGDGAKNGSEQCDPKDPNKTGWGNGGCDASCKPINTIIPKADCDATFYGKLRHGRGYVFDDQITGTPTKSRYLKDFKVDHREQLDFNGADPFPSFEWTKEIKNQNMILNPNQTIKILTATSPYYVREKPIKRDKNNLYLEYTIWYSDSQNGAWSAEKECKYYEITRCGDGILDPEYGETCDPNDPSKTGWGKGGCDENCKPKDIPQIPGQCNSQYNGQTVENLTGGNHLCTTGTMTQFQFNSATNRWTWKCDGMN", "aa_hexdigest": "b9502ac7349c2bc1e55676a9b231ac31", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 43729.08579999997, "isoelectric_point": 6.259700965881348 }, "id": "NKOCPLAKPK_1603", "locus": "GCA003260355_01583" }, { "type": "cds", "sequence": "contig_1", "start": 848139, "stop": 848240, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATGATGAAAAAAATGTTACTTTCCATGGTTATGATAGGAATGACCGTATTAGGAATGACAACCTATGCCAACGATGTTTGTGATCAGGTTTTCTATTGA", "aa": "MMMKKMLLSMVMIGMTVLGMTTYANDVCDQVFY", "aa_hexdigest": "3e7f96e43f156fb3ec0e6d26c0d456df", "start_type": "ATG", "rbs_motif": "TTTA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3797.7214999999997, "isoelectric_point": 5.710863685607911 }, "id": "NKOCPLAKPK_1604", "locus": "GCA003260355_01584" }, { "type": "cds", "sequence": "contig_1", "start": 848454, "stop": 848939, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTCTGGAGCTGGTGGGATCGTCGGAGTTGGTGGGATCGTCGGAGTTGGCGGGATCGTTGGAGTTGGCGGGATCGTTGGAGTTGGAGGGATCGTTGGAGTTGGTGGAATCGTTGGAGTTGGTGGGATCGTTGGGATCTCTGATTCTCATGGTGTAGTATGTGGTTTTTCTGGAGTTGGAGTCGGCTTTGGCGTTGGCTCTGGTTTTGGCTCCGGTTTTGGAGTTGGCTCTGGTTTTGGCGTTGGCTCTGGTTTTGGAGTTGGTTTTGGAGTTGGTTTTTTCTCTGGTTCTGGAGTTGGCTCTGGCCTTGGCGTTTCCACTTTTGGAGTTGGTGTTGGTGTTGGCTCTGGTGTTGGTGTTGGCTCTGGTTTTGGCGTTTCTTTCTCCTTCTTTCCTCCTCAGAAGCTGATACCAAGATTCACATCCTGTTGCTCAATACCTGCCTGTGCCATCGTCTCTCAGACATTCCATGTCATCGTTCCTTGA", "aa": "MSGAGGIVGVGGIVGVGGIVGVGGIVGVGGIVGVGGIVGVGGIVGISDSHGVVCGFSGVGVGFGVGSGFGSGFGVGSGFGVGSGFGVGFGVGFFSGSGVGSGLGVSTFGVGVGVGSGVGVGSGFGVSFSFFPPQKLIPRFTSCCSIPACAIVSQTFHVIVP", "aa_hexdigest": "f179b90de2b1eba4d1c489d1055285bd", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14553.538399999994, "isoelectric_point": 7.709663581848145 }, "id": "NKOCPLAKPK_1605", "locus": "GCA003260355_01585" }, { "type": "cds", "sequence": "contig_1", "start": 848912, "stop": 849022, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGAGAGTGTGCCAATGAATCTATTGGTCTCAAGATCAAAGGACTCAAAGTGATAGATAAAGTGCATATGGACGATGAGGCTCAAGGAACGATGACATGGAATGTCTGA", "aa": "MGECANESIGLKIKGLKVIDKVHMDDEAQGTMTWNV", "aa_hexdigest": "52780274d3d7ccb3c1275b7daf4f2438", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3962.5503, "isoelectric_point": 4.950073814392089 }, "id": "NKOCPLAKPK_1606", "locus": "GCA003260355_01586" }, { "type": "cds", "sequence": "contig_1", "start": 849069, "stop": 849176, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAAGAGCTTTTCAGGCGATTAATCCCTCTATCATTTCATTTGAAATGTTATTTTTTTGTCCTTCAGGTGCCACAGTAGCAACGAAGTTTCTGACTGTCTCATAG", "aa": "MSRAFQAINPSIISFEMLFFCPSGATVATKFLTVS", "aa_hexdigest": "cb81c71cb14b9974ea01cafa46286451", "start_type": "ATG", "rbs_motif": "TTTA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3813.4645, "isoelectric_point": 7.975980186462403 }, "id": "NKOCPLAKPK_1607", "locus": "GCA003260355_01587" }, { "type": "cds", "sequence": "contig_1", "start": 849248, "stop": 849736, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTAAAGATTTGAGAGCTTTCTTGGAAAAAGCAGCGGTAAAGCGAGAGGATCTCGTAGTAAATGCTGCAAGTAATAATATGGCAGGAGTAGCTGAAAGTATCGCTAAGGCAGAAGTAAATAAATACCTTATCATTGATAGACTAAAAGCAATTTTTGCCACAGAGAGAAAGATTACTGACCTTTCTTCACTCAAGGCAATCGTAGATAAGAGAAATAATGCATATCAAGATCTAATGGGTCCAAATGGAAAAACACTTTCTCAGCTTGGAATCAATATAGATAGCAAGGCATTTGCCAATACGCTCGAAAATATCGATCAAGTAGTAGATCCAGGCTTGGTAGGATTCACCGCCTTCTATAGAAAGGGAACCCAGGAAGGAAGAGGACTTTCTCTTACCGCTCTAGGAGAGACAAAGGTGCTTGGAGGAAAAATGCAAAAGCTGACTGGAGATCAGGAGCAGGCTGCCAAAGAGCGATTCTTCTGA", "aa": "MFKDLRAFLEKAAVKREDLVVNAASNNMAGVAESIAKAEVNKYLIIDRLKAIFATERKITDLSSLKAIVDKRNNAYQDLMGPNGKTLSQLGINIDSKAFANTLENIDQVVDPGLVGFTAFYRKGTQEGRGLSLTALGETKVLGGKMQKLTGDQEQAAKERFF", "aa_hexdigest": "2b09d06417372347cfff999fe9ac856f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17729.157299999977, "isoelectric_point": 9.271407890319825 }, "id": "NKOCPLAKPK_1608", "locus": "GCA003260355_01588" }, { "type": "cds", "sequence": "contig_1", "start": 849755, "stop": 849868, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCAAGCACTTTCAAAGCTGGATGATACGCATCTTACAGCACTCAATAGCTTCCTTGCAAATACAGGAGCGACCAAGAATGATTATCAAAAAGCAGCTCAAGAATTTTTGTGA", "aa": "MQALSKLDDTHLTALNSFLANTGATKNDYQKAAQEFL", "aa_hexdigest": "70d26849b1e8996c8f549f9da64a7ff6", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4070.4943999999996, "isoelectric_point": 5.4106969833374015 }, "id": "NKOCPLAKPK_1609", "locus": "GCA003260355_01589" }, { "type": "cds", "sequence": "contig_1", "start": 850130, "stop": 850552, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGACAAATATTCTGAGAAAGGCAAATATCTTGACTCAGCAGGAAGAAATCAAAAAAAATGGAGACTTCGTTGAAATCCCAGTTACAGTATTGAGAAAGGCTGGTTTGACAGTTAGAGCTCATGATGACCTTAAAGGATACGTAAAAAATGAGAATGGTATCTTGAAGTTTCCAGCAAATACGGATTACCGTTTCCTTACTGCTGCGAGAACGACAGGAGCAACCAATATTCTCGATATCGGAGGAAAAGATAATAGAGATGGAAAGATGAGAACACTGACTCCAGCTAGCGTCAAAGCTTTTGAAGGTAATAAAGAATATATCGGAGCAGTATTGGAGTCGAATGCCAAGAAGGCTTTAGACCAGATTAAAGCTAATAATCCAGAGCTTAAAGACCTTACTATGAAGATCGATGAGCAGTGA", "aa": "MTNILRKANILTQQEEIKKNGDFVEIPVTVLRKAGLTVRAHDDLKGYVKNENGILKFPANTDYRFLTAARTTGATNILDIGGKDNRDGKMRTLTPASVKAFEGNKEYIGAVLESNAKKALDQIKANNPELKDLTMKIDEQ", "aa_hexdigest": "56dbad5a84f73674611aee45ea8fbe9f", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15532.591799999977, "isoelectric_point": 9.271794700622557 }, "id": "NKOCPLAKPK_1610", "locus": "GCA003260355_01590" }, { "type": "cds", "sequence": "contig_1", "start": 850565, "stop": 851104, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGACGCTCATTGGACCAATCCCATCATGGGGACTCAATGTAGGATTTGAGAAATCTCAGCTTAAAGGTATCGAAAAGCACTACAAAAAGATAAAAGAAGAAGGAGTGCAAATGATGCAAGACCTTTTATCGCTTGATAAAGAAGGAAAACCTACTTTTGATGCAGTAAAAAAATACTTACAGCAAAAATATCCAGAATCTGATACGGCATCTCTCCAGAAAGCAGCAGAAAATCTGACTTCAGTTTTTGCTCTTGTTGACGGAATTGAGAAAGATGATGCAGCAAAGCTTATAGCAGAGCGATATGCTGATAGCTGGAGAAATAATGCTATCCAAGGACTTCCAAAAGGATGGAAACCTACGGGAGCAGCACTAGGAGTTCAATTCTTGGCAGGATTCTGGCCAGTGGGAACACTAAGTGTTAAGCTTAGTAAGTATCACAATGAAGGATATGAAGACACCAAAAATAGTCTCAAAAACCTCAGACAGCGTGCAGAATATGGTATCGGAAACGAAACTCTTCCTGGTGTTGAAGATTGA", "aa": "MTLIGPIPSWGLNVGFEKSQLKGIEKHYKKIKEEGVQMMQDLLSLDKEGKPTFDAVKKYLQQKYPESDTASLQKAAENLTSVFALVDGIEKDDAAKLIAERYADSWRNNAIQGLPKGWKPTGAALGVQFLAGFWPVGTLSVKLSKYHNEGYEDTKNSLKNLRQRAEYGIGNETLPGVED", "aa_hexdigest": "849753fcaa42c51db20d86dee89dd3ea", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19890.367999999995, "isoelectric_point": 6.758292579650879 }, "id": "NKOCPLAKPK_1611", "locus": "GCA003260355_01591" }, { "type": "cds", "sequence": "contig_1", "start": 851267, "stop": 853075, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAGTAAAAAATGGATCACAGGACTCAGCACAAGACAAGAAGATCTATGTCGGTGTAGAAGCCTCAAACACCCAAGAGCAGCTTGCAGATGATTCGGCAACCAAAGCGCTTCAGGATTTGATGAAAATGAGGGAAGAGGGCTTGATCAACGAAAAGCATTTTAAAAAAGTGCTAAAACATGACATTCAGAAGAGACTGGAAAGTTTTTTCAATAAGTTGACCAAAGAGCAGGTCAAGCAGCTCTACAAATCTGCCAAAACAGACCAAGAAAAGGCAGCTCTCCAGCTTCTCTTTAAAGAATATAAAGAGCAAAATAAGCTCGAAAAGCAAAAAAATGCTGAAATCAAAAGAGCAATCGAAGAGGCTAAAGTAGAAGCCGATGTCCTAAGAGGAGAAATAGAAAACTCAGCATCCTACAAGGCAGAGAATTACCTCCAGAGGGTGAAAAACAATACCGTTTCTGATACTGAACTCAAGAATTTTGATAAAATTATCAACGGAAAAACCCTTGCTGAGGGAAAGCTCAATAAACAGGAAAAAAGCTACAAATCAGCTTTTAGAAAGGCTTTTGTTCAAAGTGGTCTTTCTGCCAAAGATTTTGTTTCTTTGCTTCATGATCAGACAACACTTCCAGACTGGAATACGATTCTTCCTAAGAGGGATGGTAGCACTTGGACCAAGAAAGAGTCCGATAATTTTGACAAAGGACTTTTAGCATATCGCGAGGCAAAAAAATCTAATTCAGAGCTTTCTAGATGGAATCAAATCATTGGAAATCTCGAAGTTGGTCTTATCAACGAGGGAAAAGAACTCCGCGCTGTCTATACAGAATCACTTAATGCACCAATCTCCAAAAAAGAGAGAAAAGCAGGGCTTCGTAATGAAGAGGCTAATCGAAAGAGTGCCTATAGAGAGAACAATGCTCAGCAGTTGGAATCAGCAAGAGCACACTGGACCAAAAAATACCTAGAACAGGGACTAAGTCAGGGATCAGCGAGAGCTGCTGCGGAGGAGCTCATCAACAAAGATATTGATAGTAAGTGGGAACAGCATCTCAACGACCTTGATCAAAAAGGAATCCAAAAGTCCGATCTTGGAAAATTCAGAGTTCTTACTGATGCAATCAAATCCAAATCTCCAGAAGAACAGATTAAAATTCTGATGACAGATACAAATTTTGATGGAATTGGAAATTATCTGGATGGACGTGGAGTGAAAAGAGGAATGCAGGTAGAAAATGCACTTTCTGTAGCTCTCAATAGAGGAGTGCCAATGGATGCTGTGGTAGAAAATGTGATTGACTTTATGAAGGTTAGAATGCCAAAATCAGACCTTTCTAAAGTAAAGACTCCTGCGGAATTTGCACAATGGATGAGTGCTGACGTAGAGAACGCTCGTGCAGTGCAGACAGCACTTATGGACTCTCCAGCAGAAACAGGTAAAATTTTCGAATATGGAAAGGACGCCGTGAAAAAAACGCTCGAGATGCAGAAATTCTCTCCAGAGGATATTAAGTTGATCAAGGAAACAATTGATGCAGCTCCTGCACTTCAGGGGCTCTCAGAGAGTGGGAAAGAACTTGTCTTGTCTAGCACTGCTTCTTACCTACTTGCAGAAATGAAGAAAAAGGATATTGTTTCAACTTCTGCAAACCTCAGCGGAGTAAGTGCTGGAATGAATTTGCCATTAGATCAGATTCTCAAAGGACTTTCTTTCAATCTGGGAACAGGAGTTACCAAAGATGGAAAACCTTTTGCAGGGGTAGGACTCGCTTGGAATAAAGAAGCAGCCAAGTGGAACCTTTGA", "aa": "MTVKNGSQDSAQDKKIYVGVEASNTQEQLADDSATKALQDLMKMREEGLINEKHFKKVLKHDIQKRLESFFNKLTKEQVKQLYKSAKTDQEKAALQLLFKEYKEQNKLEKQKNAEIKRAIEEAKVEADVLRGEIENSASYKAENYLQRVKNNTVSDTELKNFDKIINGKTLAEGKLNKQEKSYKSAFRKAFVQSGLSAKDFVSLLHDQTTLPDWNTILPKRDGSTWTKKESDNFDKGLLAYREAKKSNSELSRWNQIIGNLEVGLINEGKELRAVYTESLNAPISKKERKAGLRNEEANRKSAYRENNAQQLESARAHWTKKYLEQGLSQGSARAAAEELINKDIDSKWEQHLNDLDQKGIQKSDLGKFRVLTDAIKSKSPEEQIKILMTDTNFDGIGNYLDGRGVKRGMQVENALSVALNRGVPMDAVVENVIDFMKVRMPKSDLSKVKTPAEFAQWMSADVENARAVQTALMDSPAETGKIFEYGKDAVKKTLEMQKFSPEDIKLIKETIDAAPALQGLSESGKELVLSSTASYLLAEMKKKDIVSTSANLSGVSAGMNLPLDQILKGLSFNLGTGVTKDGKPFAGVGLAWNKEAAKWNL", "aa_hexdigest": "fbdf4ac237cc13ac618c3605fa7257f0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 67387.71960000033, "isoelectric_point": 9.050603675842286 }, "id": "NKOCPLAKPK_1612", "locus": "GCA003260355_01592" }, { "type": "cds", "sequence": "contig_1", "start": 853128, "stop": 853421, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MNK7", "UniRef:UniRef90_A0A660MNK7" ], "nt": "ATGCAAACAATGACAAAACAAGAGTACATCACTACCTTCTTAGAAAAAGTAAAAGAAGTCTGGGAGCCAGCGAGAGGTTTTCTCGTATTAATGAGGTATAATATCCTCAGAGAAGAACAAATTGATGAGCTTTTCCAGGTCTTTAAAAAGGTAGTGCAATCCACTTCTGATGAACAAAAAAAGAGAAAACTTCAAAGAGCGATCTCTGTCGTAGAAAAACTCAAACAACAAGAACAAAACGCAGAAGCAAACAACTTCTCTTCAGAGTTAGATTCGATTCTCTCTGAACAATAA", "aa": "MQTMTKQEYITTFLEKVKEVWEPARGFLVLMRYNILREEQIDELFQVFKKVVQSTSDEQKKRKLQRAISVVEKLKQQEQNAEANNFSSELDSILSEQ", "aa_hexdigest": "e12b369e3120d0db157bd2bdbbb08b61", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A660MNK7", "query_cov": 0.9690721649484536, "subject_cov": 1.0, "identity": 1.0, "score": 172.0, "evalue": 3.93e-54, "valid": true, "uniref50_id": "UniRef50_A0A660MNK7" }, "pscc": { "uniref50_id": "UniRef50_A0A660MNK7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MNK7" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11543.04899999999, "isoelectric_point": 5.560240364074706 }, "id": "NKOCPLAKPK_1613", "locus": "GCA003260355_01593" }, { "type": "cds", "sequence": "contig_1", "start": 853587, "stop": 853982, "strand": "-", "frame": 2, "gene": null, "product": "Adenylate kinase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7W4HH15", "UniRef:UniRef90_A0A660MQX8" ], "nt": "ATGCTGGATGATAATCTGGTTTTTGATCTCTTTAATATGTATTATCATCTCTTAACAGACAATGATCTTATCTTGGCGGATGGCTTCCCAAGGACGATCCCTCAGATGTATTATTTTCTTAGTAAGGAATGTAAGTACAAAAGAGAGTTCCTCGGGGTTTACTTTGATATCCCGAGAGAAAAGGCAGTAGAGAGGATCCTTTCCAGAGCAAAAATTCAAAATCGTATTGATGATCTGAATATGGATACGATTAATCAGAGATTGGACCTCTTTGAGGAGGAAACCTATCCTGTGATCGCCTATTTCAAAAGTATTGGAAAGCTGATTACGATCGACGCTCAGCAAACCGTAGAAGAAATCTTCGCTCAGACGAAGCAAGCAATTTTGGCTAGATAA", "aa": "MLDDNLVFDLFNMYYHLLTDNDLILADGFPRTIPQMYYFLSKECKYKREFLGVYFDIPREKAVERILSRAKIQNRIDDLNMDTINQRLDLFEEETYPVIAYFKSIGKLITIDAQQTVEEIFAQTKQAILAR", "aa_hexdigest": "2d7f244c71eca68d70fa789f10bf7382", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 853587, "stop": 854162, "observations": { "mutation": [ 854060 ], "stop": [ 854060 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A660MQX8", "query_cov": 1.0, "subject_cov": 0.6858638743455497, "identity": 1.0, "score": 264.0, "evalue": 1.4e-88, "gene_start": 1, "gene_end": 131, "reference_start": 61, "reference_end": 191, "reference_sequence": "MQKDIILFGIQGSGKGTQAQLLLKEFPALKYLESGNVFRAITSNNNIISEYIKGRIREGKMLDDNLVFDLFNMYYHLLTDNDLILADGFPRTIPQMYYFLSKECKYKREFLGVYFDIPREKAVERILSRAKIQNRIDDLNMDTINQRLDLFEEETYPVIAYFKSIGKLITIDAQQTVEEIFAQTKQAILAR" }, "paralog": false, "identity": 0.9947643979057592, "score": 374.0, "evalue": 1.6e-134, "description": "Internal stop codon at 854060. Nonsense mutation around 854060.", "psc": { "uniref90_id": "UniRef90_A0A660MQX8", "product": "Adenylate kinase", "uniref50_id": "UniRef50_A0A7W4HH15", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A660MQX8", "UniRef:UniRef50_A0A7W4HH15" ] }, "pscc": { "uniref50_id": "UniRef50_A0A7W4HH15", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7W4HH15" ], "product": "Adenylate kinase" } }, "id": "NKOCPLAKPK_1614", "locus": "GCA003260355_01594" }, { "type": "cds", "sequence": "contig_1", "start": 854058, "stop": 854162, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAAAAGACATCATCCTTTTTGGGATCCAAGGATCAGGAAAGGGGACGCAAGCACAATTACTCCTCAAAGAGTTTCCAGCCCTCAAATATCTCGAATCATGA", "aa": "MQKDIILFGIQGSGKGTQAQLLLKEFPALKYLES", "aa_hexdigest": "1b14b12a7394c2ef1aa0334bac7702d2", "start_type": "ATG", "rbs_motif": "TTCTA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3766.4066000000003, "isoelectric_point": 8.139923286437988 }, "id": "NKOCPLAKPK_1615", "locus": "GCA003260355_01595" }, { "type": "cds", "sequence": "contig_1", "start": 854218, "stop": 854607, "strand": "-", "frame": 1, "gene": "yihA", "product": "ribosome biogenesis GTP-binding protein YihA/YsxC", "db_xrefs": [ "GO:0003924", "GO:0005525", "GO:0005737", "GO:0042254", "GO:0043022", "SO:0001217", "UniRef:UniRef50_A0A2W4ILK3", "UniRef:UniRef90_A0A328EKS6" ], "nt": "GTGAATGAAAACCGATATTTCGTAGATTTGCCAGGATATGGCTATGCAAAGGCGAGTCTGGAGCAGAGGAGGAAATGGATAGATGAAATTCATCACTATTTCCTAGAGAGGAAGCCTTTTATTCTCCTGCTCATTGACGGTTCGATTCCGCCTCAAAAGATTGATCTAGAGTTTGTATCGGCACTTACAGCAGAAAATCTGAATTTTGCACTCATTTTGACCAAGACCGATAAAGCAAATCAAAAAACTCTGCATCAAAACCTTAAACTCATCAAACAGGCTCTCCAAAAGCAAATCGGTAAACTCCCAGAGATGCTCCTTTCTTCCACGACCAAAAGGCAAGGGAAAGAGCAGATTTTAGACTTTATTCAAAAAAAACTTGCATCCTAG", "aa": "MNENRYFVDLPGYGYAKASLEQRRKWIDEIHHYFLERKPFILLLIDGSIPPQKIDLEFVSALTAENLNFALILTKTDKANQKTLHQNLKLIKQALQKQIGKLPEMLLSSTTKRQGKEQILDFIQKKLAS", "aa_hexdigest": "9c353b6d9d9038a5c8ab414496e2a5a3", "start_type": "GTG", "rbs_motif": null, "genes": [ "yihA" ], "truncated": "3-prime", "pseudogene": { "start": 854218, "stop": 854802, "observations": { "mutation": [ 854637 ], "stop": [ 854637 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EKS6", "query_cov": 1.0, "subject_cov": 0.6649484536082474, "identity": 0.9690000000000001, "score": 245.0, "evalue": 2.94e-81, "gene_start": 1, "gene_end": 129, "reference_start": 66, "reference_end": 194, "reference_sequence": "MLIHSLAFVKSASKLSECPSSNFPEVAVIGRSNVGKSSLINFLMGKTIAKASDKPGKTQLINYFLVNESRYFVDLPGYGYAKASLEQRRKWIDEIHHYFLERKPFILLLIDGSIPPQKMDLEFVSALTAENLNFALILTKTDKANQKTLHQNLKLLKQALQKQIGKLPEMLLSSTTKRQGKEQILDFIQKKLAS" }, "paralog": false, "identity": 0.9587628865979382, "score": 366.0, "evalue": 3.66e-131, "description": "Internal stop codon at 854637. Nonsense mutation around 854637.", "psc": { "uniref90_id": "UniRef90_A0A328EKS6", "gene": "yihA", "product": "ribosome biogenesis GTP-binding protein YihA/YsxC", "uniref50_id": "UniRef50_A0A2W4ILK3", "go_ids": [ "GO:0003924", "GO:0005525", "GO:0005737", "GO:0042254", "GO:0043022" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EKS6", "UniRef:UniRef50_A0A2W4ILK3", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0042254", "GO:0043022" ] }, "pscc": { "uniref50_id": "UniRef50_A0A2W4ILK3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4ILK3" ], "product": "Probable GTP-binding protein EngB" } }, "id": "NKOCPLAKPK_1616", "locus": "GCA003260355_01596" }, { "type": "cds", "sequence": "contig_1", "start": 854635, "stop": 854802, "strand": "-", "frame": 1, "gene": null, "product": "50S ribosome-binding GTPase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A928RXG9" ], "nt": "ATGCTGATTCAATCGCTTAGCTTTGTAAAATCAGCTTCCAAAATCACTGAATGTCCTAGCTCAAATTTCCCAGAGGTTGCGGTGATTGGAAGATCTAATGTCGGGAAATCTTCGCTCATCAACTTCTTAATGGGCAAAACTATTGCGAAAGCTTCTGATAAACCTTGA", "aa": "MLIQSLSFVKSASKITECPSSNFPEVAVIGRSNVGKSSLINFLMGKTIAKASDKP", "aa_hexdigest": "37d014c6499ca5a775f5f20c9c324d35", "start_type": "ATG", "rbs_motif": "TTATT", "psc": { "uniref90_id": "UniRef90_A0A928RXG9", "query_cov": 0.8363636363636363, "subject_cov": 1.0, "identity": 0.5429999999999999, "score": 52.4, "evalue": 5.38e-08, "valid": false, "product": "50S ribosome-binding GTPase", "uniref50_id": "UniRef50_A0A928RXG9" }, "pscc": { "uniref50_id": "UniRef50_A0A928RXG9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A928RXG9" ], "product": "50S ribosome-binding GTPase" }, "genes": [], "id": "NKOCPLAKPK_1617", "locus": "GCA003260355_01597" }, { "type": "cds", "sequence": "contig_1", "start": 854825, "stop": 855094, "strand": "-", "frame": 3, "gene": null, "product": "Plasmid stabilization protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1V5ZQW6", "UniRef:UniRef90_A0A328EJR5" ], "nt": "ATGTATCAGATTTATTTAGAAAAGAAGGTCCAAAAATTCCTATTGAAACATCAAGGGCAGCCAATTATTACTCAGTTTAAGCAGGCTTTGACTCAACTGGCGCTTGATCCCTATCAGAATACCTTGGATATCAAAGTCCTTGTTGGTTTGCCTCATTGTTATAGGCTTAGGATCGGAAAGTGGAGATTCCTTTATGAGATACTTGATCAAAAGCTGATTATTTCTTTCTTTGATGCTGGTCCAAGAGGGCAGGTCTATAAAGAAGTTTAA", "aa": "MYQIYLEKKVQKFLLKHQGQPIITQFKQALTQLALDPYQNTLDIKVLVGLPHCYRLRIGKWRFLYEILDQKLIISFFDAGPRGQVYKEV", "aa_hexdigest": "0e4ab444e6cc80648264de4a89e8d5b3", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A328EJR5", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.9440000000000001, "score": 177.0, "evalue": 1.24e-56, "valid": true, "product": "Plasmid stabilization protein", "uniref50_id": "UniRef50_A0A1V5ZQW6" }, "pscc": { "uniref50_id": "UniRef50_A0A1V5ZQW6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1V5ZQW6" ], "product": "Plasmid stabilization system protein" }, "genes": [], "id": "NKOCPLAKPK_1618", "locus": "GCA003260355_01598" }, { "type": "cds", "sequence": "contig_1", "start": 855098, "stop": 855367, "strand": "-", "frame": 3, "gene": null, "product": "Antitoxin", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBF6472", "UniRef:UniRef100_A0A328EL22", "UniRef:UniRef50_A0A1V5ZQJ4", "UniRef:UniRef90_A0A2W4IKF8" ], "nt": "ATGCTTACAGCTAGCAGTTTTGCTTCTATTTCAGATCTCAGAGATAAGACGGCAAGTATCGTGAAACAGGTCAATACGACAGGAAAAAAAATTATCCTTTCACAGAATAAGCCGGTTGGGGTTTTTCTCAGTATTGATGAATATAATGCACTTCTTAAGCTGAGTTTCCCTGCTGAAAAAGCAACTCCTCAGGAAATAAAGGCTTATCAGGCAAGCTCACATGGAGAGGAGGCAGTAGAAGCCTTCGCTTTTTTAGATGCTTTCCAATAG", "aa": "MLTASSFASISDLRDKTASIVKQVNTTGKKIILSQNKPVGVFLSIDEYNALLKLSFPAEKATPQEIKAYQASSHGEEAVEAFAFLDAFQ", "aa_hexdigest": "6e3acb1b2e0452da34a30ee74f9344a4", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000DBF6472", "uniref100_id": "UniRef100_A0A328EL22", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBF6472", "UniRef:UniRef100_A0A328EL22" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EL22", "uniref90_id": "UniRef90_A0A2W4IKF8" }, "psc": { "uniref90_id": "UniRef90_A0A2W4IKF8", "product": "Antitoxin", "uniref50_id": "UniRef50_A0A1V5ZQJ4" }, "pscc": { "uniref50_id": "UniRef50_A0A1V5ZQJ4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1V5ZQJ4" ], "product": "Antitoxin" }, "genes": [], "id": "NKOCPLAKPK_1619", "locus": "GCA003260355_01599" }, { "type": "cds", "sequence": "contig_1", "start": 855485, "stop": 855859, "strand": "-", "frame": 3, "gene": null, "product": "Endonuclease/exonuclease/phosphatase domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EHL1", "UniRef:UniRef90_A0A328EHL1" ], "nt": "TTGACCGTTGCTACAGCTCATAAGTCTTATAGTTCAGCATTTGCAGAAGATCAAGATAAAATAGATGAAACTAAAAAACTGATTGAGTGTTTTAAGCAGTATCCAGAGAATCTCATCTTTACAGGTGATCTTAATTTGCCTCCAGAAAGTACATCTCTTCAAATGCTAGAAAAAGAGCTTCAGCATTGTGGACCTGATTATTCACAAGCAACTCGAACGACGAAGCCTTTTTCTTTTATGGGTTTTGAAGAAAAAGAACTCAGATGGAGATTGGATTATGTTTTTGCTTCTAAGAATATTAAAGTTCTTTCATCACAGATTATTCCTACAGAATGGAGCGATCATCTTCCAGTTCTTGTTGAGTGTGAGTTTTAA", "aa": "MTVATAHKSYSSAFAEDQDKIDETKKLIECFKQYPENLIFTGDLNLPPESTSLQMLEKELQHCGPDYSQATRTTKPFSFMGFEEKELRWRLDYVFASKNIKVLSSQIIPTEWSDHLPVLVECEF", "aa_hexdigest": "ba8b65a86b15322eb4ea1bc8d3c4d6fd", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 855485, "stop": 856159, "observations": { "mutation": [ 855997, 856117 ], "stop": [ 855997, 856117 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EHL1", "query_cov": 1.0, "subject_cov": 0.496, "identity": 0.992, "score": 254.0, "evalue": 4.83e-84, "gene_start": 1, "gene_end": 124, "reference_start": 127, "reference_end": 250, "reference_sequence": "MKVKVLQRNVWFRERAENLLTLMKELNPDIICCQEITVGSGFNDKRDVAQFIAQELGYHYYYSKAHKYEYPFTPLGEVNFGGNAIFSRFPIVESFDFALINPEDNPDHPYERRTCAVARIQAEDTLLTVATAHKSYSSAFAEDQDKIDETKKLIECFKQYPENLIFTGDLNLPPESTSLQMLEKELQHCGPDYSQATRTTKPFSFMGFEEKELRWRLDYVFASKNIKVLSSQIIPTEWSDHLPVLVECEF" }, "paralog": false, "identity": 0.9910714285714286, "score": 459.0, "evalue": 2.34e-167, "description": "Internal stop codon at 855997, 856117. Nonsense mutation around 855997, 856117.", "psc": { "uniref90_id": "UniRef90_A0A328EHL1", "product": "Endonuclease/exonuclease/phosphatase domain-containing protein", "uniref50_id": "UniRef50_A0A328EHL1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EHL1", "UniRef:UniRef50_A0A328EHL1" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EHL1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EHL1" ], "product": "Endonuclease/exonuclease/phosphatase domain-containing protein" } }, "id": "NKOCPLAKPK_1620", "locus": "GCA003260355_01600" }, { "type": "cds", "sequence": "contig_1", "start": 856115, "stop": 856372, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTTCAGCTTTTTTTTCTTGAAAAACTAGGAAGAAAATATATAAAGAAACTACTATCCTATCTCAATAAAGGGAAAGTTGCAGGTGTAATCTTGTATTTTGCAGGGCAGCTTTATTATCTAATAAATTGTAGAATGAAAGTAAAAGTTTTACAACGAAATGTGTGGTTTAGGGAAAGAGCTGAAAATCTACTTACTTTGATGAAAGAGCTTAATCCTGATATTATCTGTTGTCAGGAGATTACGGTAGGTTCGTGA", "aa": "MVQLFFLEKLGRKYIKKLLSYLNKGKVAGVILYFAGQLYYLINCRMKVKVLQRNVWFRERAENLLTLMKELNPDIICCQEITVGS", "aa_hexdigest": "5e2576a0cce06e97b23e86439a62bb2e", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10019.006299999994, "isoelectric_point": 9.675689125061037 }, "id": "NKOCPLAKPK_1621", "locus": "GCA003260355_01601" }, { "type": "cds", "sequence": "contig_1", "start": 856402, "stop": 856680, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328ELL1", "UniRef:UniRef90_A0A328ELL1" ], "nt": "TTGTTGGCATTCTGGGGGATTACCGTTTTGCCGGTTAGATATGAAAAATTTTCCAAAGTAAATCCCGCTACCTTAAATAGCATAATCTGGCTTGGAACTATTAGTATTTTGGTTGTTGCTTTTCTTTCGTATCTGACAATTTGGAATTTAATGGAAGCGGATGACTTCTATTGGAAAGTGATTTCTACGATTCTTATCGTTAACTTCGTAGCTTTTGCTTGTGTTTTCTCTCTCGAAAAATGGGGCGGGAATGATGATAAGAAAGATAAAGACCTCTAA", "aa": "MLAFWGITVLPVRYEKFSKVNPATLNSIIWLGTISILVVAFLSYLTIWNLMEADDFYWKVISTILIVNFVAFACVFSLEKWGGNDDKKDKDL", "aa_hexdigest": "33cacbcab6ec8b0ac62192a74a23671d", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 856402, "stop": 856827, "observations": { "mutation": [ 856689, 856734 ], "stop": [ 856689, 856734 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328ELL1", "query_cov": 1.0, "subject_cov": 0.6524822695035462, "identity": 0.9890000000000001, "score": 188.0, "evalue": 5.58e-60, "gene_start": 1, "gene_end": 92, "reference_start": 50, "reference_end": 141, "reference_sequence": "MTKFGGTNKFFLYVAIGGGLCIGLLSLLSIRGNFGGDVILQGLTTYGVTLLAFWGITVLPVRYEKFSKVNPATLNSIIWLGTISILVVAFLSYLTIWNLMEADDFYWKVISTILIVNFVAFACVFSLEKWGGNDDKKDKDL" }, "paralog": false, "identity": 0.9858156028368794, "score": 280.0, "evalue": 8.46e-99, "description": "Internal stop codon at 856689, 856734. Nonsense mutation around 856689, 856734.", "psc": { "uniref90_id": "UniRef90_A0A328ELL1", "uniref50_id": "UniRef50_A0A328ELL1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328ELL1", "UniRef:UniRef50_A0A328ELL1" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328ELL1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328ELL1" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 10568.32439999999, "isoelectric_point": 5.125763130187988 }, "id": "NKOCPLAKPK_1622", "locus": "GCA003260355_01602" }, { "type": "cds", "sequence": "contig_1", "start": 856732, "stop": 856827, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAAAATTTGGAGGAACAAACAAATTCTTTCTTTATGTCGCAATTGGAGGTGGTTTGTGTATTGGGCTTTTATCGCTATTATCTATTCGATGA", "aa": "MTKFGGTNKFFLYVAIGGGLCIGLLSLLSIR", "aa_hexdigest": "f3d988755b29425535a7c448ba308ebf", "start_type": "ATG", "rbs_motif": "TTATT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3290.9792000000007, "isoelectric_point": 9.78522090911865 }, "id": "NKOCPLAKPK_1623", "locus": "GCA003260355_01603" }, { "type": "cds", "sequence": "contig_1", "start": 857111, "stop": 857725, "strand": "-", "frame": 3, "gene": null, "product": "Lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MYN6", "UniRef:UniRef90_A0A660MYN6" ], "nt": "TTGCTCTATCGCAAAGATCAGCTCATCGCAACTGGCGATAACCTTTTTTACTTTCTGACCGATAAGACGCATATTATCCTCTGTTCAAAGAGAGACGCCTATCTATGTTTCATTGATGACGCCTGGAAAGCAGTCGAAACGACTTCGGCTCTTCCAAAGCTCTTCAAAAAAAAGGTCTATTATCTGACGACTGATCCGAATACGATGACGCACCAGCTCTTCGAGAATGATCGCTTGCTGTATTCTTTCAGTGCCGATCATCCAACAGGCGAACCCTTGCTCTCTCTTGAGGTTAATGAAGGAGGACGGTGGATTTCCTATATTGCGCTCAACCTAGAGCAGGAAAAACTTGAGTTTCATTTGATTCATAATAGAAAAAATCTGATTGCATCCGAGGGGCGAACCGATGCCTTCGAGCTCAGGCAACTCAAAGGCAAACTCTTCTACTTCTACCAAAAAGATGGAAAAATATATTACTCTCTTGCTGGAAAAAGTTACAGAACACCATTTCAGGAGGTTATTCACGATGGATGTTGTTCGGCAGGAGTATTTTCGCTCCGTTTCTTAGGAGATTCCTTCCTCCTCTATGGGAAAAAGAATGGAAGATTCTATTGA", "aa": "MLYRKDQLIATGDNLFYFLTDKTHIILCSKRDAYLCFIDDAWKAVETTSALPKLFKKKVYYLTTDPNTMTHQLFENDRLLYSFSADHPTGEPLLSLEVNEGGRWISYIALNLEQEKLEFHLIHNRKNLIASEGRTDAFELRQLKGKLFYFYQKDGKIYYSLAGKSYRTPFQEVIHDGCCSAGVFSLRFLGDSFLLYGKKNGRFY", "aa_hexdigest": "9ede3ba98f8f7d96a76a70d15f532980", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 857087, "stop": 858001, "observations": { "mutation": [ 857113, 857737, 857989 ], "stop": [ 857113, 857737, 857989 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A660MYN6", "query_cov": 1.0, "subject_cov": 0.6710526315789473, "identity": 0.98, "score": 417.0, "evalue": 4.75e-146, "gene_start": 1, "gene_end": 204, "reference_start": 93, "reference_end": 296, "reference_sequence": "MRWIGCFFLLILGACTPFHYPESALPLDFYEITGSWASLRELDQEFYSPLDEYFSGEIDRYQRQELNALSDTTIKEALEGSDFSFETSGNKWLLYRKDQLIATGDNLFYFLTDKTHIILCSKRDAYPCFIDDAWKAVETTSALPKLFKKKVYYLTTDPNTMTHQLFENDRLLYSFSADHPTGEPLLSLEVNEGGRWISYIALNLEQEKLEFHLVHNRKNLIASEGRTDAFELRQLKGKLFYFYQKDGKIYYSLAGKSYGTPFQEVIHDGCCSAGVFSLRFLGDSFLLYGKKNGRFYGYQVKFSL" }, "paralog": false, "identity": 0.9802631578947368, "score": 613.0, "evalue": 6.57e-226, "description": "Internal stop codon at 857113, 857737, 857989. Nonsense mutation around 857113, 857737, 857989.", "psc": { "uniref90_id": "UniRef90_A0A660MYN6", "product": "Lipoprotein", "uniref50_id": "UniRef50_A0A660MYN6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A660MYN6", "UniRef:UniRef50_A0A660MYN6" ] }, "pscc": { "uniref50_id": "UniRef50_A0A660MYN6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MYN6" ], "product": "Lipoprotein" } }, "id": "NKOCPLAKPK_1624", "locus": "GCA003260355_01604" }, { "type": "cds", "sequence": "contig_1", "start": 858072, "stop": 858638, "strand": "-", "frame": 2, "gene": null, "product": "Methyltransferase domain-containing protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBFEF03", "UniRef:UniRef100_A0A328EL41", "UniRef:UniRef50_A0A328EL41", "UniRef:UniRef90_A0A328EL41" ], "nt": "ATGCGATTAGAAGAAAAGCTAGCAACACTCTTCCATCACCAAAAAATTTCTGCAGTGCTTGATCTCGGAGCAGGCGATGGAAGGTGGAGTATTTTCTGTGCGAGCTATGGTGCGAAAGTAGTCGCAATCGACAATCAATCCAAGCCTGATCGGCAATTCCCTGAGTATTTGACTCTTCATCCAGCGATACATTTCTTTCAAGCTGATTTAAAGGAGTTGCCTCGTCCTTGCGACCAAAGATACGATCTAATCTTGCTCTTCAATGTTATCATCTTTCTCAAAAAGAGTTTTGTTCTCTCTCAATTGCTTCCTTATGCTCTTTCACAGCCGAATCCTGAAGGAAGACTTTGTTTGACCTTTTTCTTTTTCGATGATCAGACGATGGGAGTCAATCAAAAGCTTTCTTTCTATACCTTTGAGGATTTTCAGTTGCCCGAGGGCTATACGCTCACGCATTGCGAAGAAAAATACCTCCAAGACAGCCATCCTCCTTACGGCGAACATCAGCATCATCTTGGTTATCTAGAAATCATAAAAAAACAGAAAAAATCTGACCATATCTTTTAA", "aa": "MRLEEKLATLFHHQKISAVLDLGAGDGRWSIFCASYGAKVVAIDNQSKPDRQFPEYLTLHPAIHFFQADLKELPRPCDQRYDLILLFNVIIFLKKSFVLSQLLPYALSQPNPEGRLCLTFFFFDDQTMGVNQKLSFYTFEDFQLPEGYTLTHCEEKYLQDSHPPYGEHQHHLGYLEIIKKQKKSDHIF", "aa_hexdigest": "7fed55b18fa2b8d2fe1f6cd518c0b599", "start_type": "ATG", "rbs_motif": "TTTTTA", "ups": { "uniparc_id": "UPI000DBFEF03", "uniref100_id": "UniRef100_A0A328EL41", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBFEF03", "UniRef:UniRef100_A0A328EL41" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EL41", "uniref90_id": "UniRef90_A0A328EL41" }, "psc": { "uniref90_id": "UniRef90_A0A328EL41", "product": "Methyltransferase domain-containing protein", "uniref50_id": "UniRef50_A0A328EL41" }, "pscc": { "uniref50_id": "UniRef50_A0A328EL41", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EL41" ], "product": "Methyltransferase domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_1625", "locus": "GCA003260355_01605" }, { "type": "cds", "sequence": "contig_1", "start": 858666, "stop": 860252, "strand": "-", "frame": 2, "gene": "alaS", "product": "alanine--tRNA ligase", "db_xrefs": [ "EC:6.1.1.7", "GO:0000166", "GO:0004813", "GO:0005737", "GO:0006419", "SO:0001217", "UniParc:UPI000DC006AD", "UniRef:UniRef100_A0A328EF36", "UniRef:UniRef50_A0A7W4ECQ1", "UniRef:UniRef90_A0A2W4IJ26" ], "nt": "ATGCAATCCCTTACTTCTCAGGAAATCAGACAAAGACGATCAGATTTTTGGACTTCTAAAGCACATACGCATTTACCAGAAGCCTCACTTATCGCAGACAAGGAATCTACTGCGCTTTTCAATGTTGCGGGAATGCAGCCGCTTATTCCTTATCTTGCTGGGAAAGAGCATCCGCTTGGACATAGGCTTTTTAATATCCAAAAATGCGTGAGAACGGTGGATATTGATGAGGTTGGAGATAGTTCACATCTAACTTTCTTTGAGATGATGGGAAACTGGAGCTTGGGTGATTATTTCAAGCAGGAGGCAATTCAATGGAGTTATGAGTTCCTCGTAGAGAAGCTGAATTTTGATCCTCGTAAATTAGCCGTAACCGTGTTTGAGGGGAATGAAGATGCTCCGAGAGATGAGATCGCTTTTGAGGCTTGGAAAAAAGCAGGGCTTCCTGAGGAGAGGATTTCTTTTTTGGATGCTAAGAATAACCGATGGAGTCCAGGTCCTGTTGGTCCTTGCGGTCCTGATACGGAGATTTACTACCGAGTGGGGGAATCAGAATTTCCACCTGTTGGTTCTAATGTAAAAACCGATGAAGCCAATTGGCTAGAAATCTGGAATAATGTATTTATGGAGTTTTATAGAGATGAAAAGGGAATCCTGACCAAGCTTTCTCAGCAGAATGTAGATACCGGAATGGGACTAGAAAGAATGTGTAAGGTTCTTCAGCACAAGGAAAGCGTTTATGAAACCGATCTTTTCGCCCCTTTCCTGCATATGCTAGAACAGGCAACCGAACTCAAGTATGCTGACCACCAGAGAAAATTCAGAATTATTGCTGATCATTTGAGAACAGCGTTTATGCTGATTAACGATGGGCTTACGCCTTCCAATGTCGGAGCAGGCTATGTTTTGAGGATGATTATCAGGAGAGGGTATTACAATCTCTTTTTGCTTAGAAAGATGATGAAGCCGGAGCTGGAGCATTTTATAGATCAGGCTTTCGTGGCGTTCAAAGGGCTTAGAGAGTTTAATGAGCCGATGATCAAAAAGGTGCTCCTTCAGGAGATCGCTCAGTTTGAGAAAACCATTCATAATGGAGAAAAAATTCTGACTGAACTCTTGGATAAGCTTACTACCGAAGGGAAGAAAACGCTCGGAGGAGATCAGATTTTTATGCTCTATGATACCTACGGTTTCCCATTAGAGATTACGAAAGAACTTGCTGCGGATCGTGGAGTTGCTTTGGATCTCGCAGGCTATGAAAAGGCTTTAGAAGCAGCAAAAGAGAAATCTCGCCAAGGAAGCAAGGCGATGTTCCAGAAATCTGCAGATTGGTCTAAGTATTTGGAGGGAGTTCCTGCGACAGAGTTTGTGGGGTATGAGAACCTGAATTTTTCTGATGCGAAGCTTTTGAAGGAGATTGATACCGAAGAGGGGCAGAAAGTGCTGATTTTTGATAAGACACCGTTCTATCCTGAGATGGGAGGTCAGAATGGAGATGCTGGAGTGATTGAGCTAGATGATGGTCGCCGTTTGGAGATTGTAAATGTGCAGAAGGTTGCAGGCGTGATTTTGCATTTTGTAGGGTAG", "aa": "MQSLTSQEIRQRRSDFWTSKAHTHLPEASLIADKESTALFNVAGMQPLIPYLAGKEHPLGHRLFNIQKCVRTVDIDEVGDSSHLTFFEMMGNWSLGDYFKQEAIQWSYEFLVEKLNFDPRKLAVTVFEGNEDAPRDEIAFEAWKKAGLPEERISFLDAKNNRWSPGPVGPCGPDTEIYYRVGESEFPPVGSNVKTDEANWLEIWNNVFMEFYRDEKGILTKLSQQNVDTGMGLERMCKVLQHKESVYETDLFAPFLHMLEQATELKYADHQRKFRIIADHLRTAFMLINDGLTPSNVGAGYVLRMIIRRGYYNLFLLRKMMKPELEHFIDQAFVAFKGLREFNEPMIKKVLLQEIAQFEKTIHNGEKILTELLDKLTTEGKKTLGGDQIFMLYDTYGFPLEITKELAADRGVALDLAGYEKALEAAKEKSRQGSKAMFQKSADWSKYLEGVPATEFVGYENLNFSDAKLLKEIDTEEGQKVLIFDKTPFYPEMGGQNGDAGVIELDDGRRLEIVNVQKVAGVILHFVG", "aa_hexdigest": "05b80aea46b28ca3cb746a0e3eb9ba50", "start_type": "ATG", "rbs_motif": "TTTTT", "ups": { "uniparc_id": "UPI000DC006AD", "uniref100_id": "UniRef100_A0A328EF36", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DC006AD", "UniRef:UniRef100_A0A328EF36" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EF36", "uniref90_id": "UniRef90_A0A2W4IJ26" }, "psc": { "uniref90_id": "UniRef90_A0A2W4IJ26", "gene": "alaS", "product": "alanine--tRNA ligase", "ec_ids": [ "6.1.1.7" ], "uniref50_id": "UniRef50_A0A7W4ECQ1", "go_ids": [ "GO:0000166", "GO:0004813", "GO:0005737", "GO:0006419" ] }, "pscc": { "uniref50_id": "UniRef50_A0A7W4ECQ1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7W4ECQ1" ], "product": "Alanine--tRNA ligase" }, "genes": [ "alaS" ], "id": "NKOCPLAKPK_1626", "locus": "GCA003260355_01606" }, { "type": "cds", "sequence": "contig_1", "start": 860766, "stop": 861062, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCCCTATGATCCGGAAAAAGGAAAGGTTCTTTGCACTGACGGTGACTATGACTATGAGGAACTGAGACGCCCAAAAGAAAAAGCCTTAGAGTCCTTTGTAAAAAAGCACGATTTACAGCATAATCCGTATTTTACTTCCCTTCTCGTTAACTTGGACAATAATACAGAAGATGAAATCGTGATTCAACAGATGACAAAGTGTGAAGAAATAGAACTTTGCAGGGACTTAGTGCCAAAAGTTCGAGGAGATAATGATAAGCATTTCTTTTTTCATACTGAAGCTTCAATGACGTGA", "aa": "MPYDPEKGKVLCTDGDYDYEELRRPKEKALESFVKKHDLQHNPYFTSLLVNLDNNTEDEIVIQQMTKCEEIELCRDLVPKVRGDNDKHFFFHTEASMT", "aa_hexdigest": "93357a4fbd371fb56a983c9e43d89dfe", "start_type": "GTG", "rbs_motif": "TTATT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11581.891299999992, "isoelectric_point": 4.881298637390137 }, "id": "NKOCPLAKPK_1627", "locus": "GCA003260355_01607" }, { "type": "cds", "sequence": "contig_1", "start": 861126, "stop": 861332, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTCTCTCATTCAGGGGAACAGCTTACTCAAGCATTCACCGGAGGAACCTGTGATACACCAAACCTTGCGGAATTGCAAAAGCCTATTCCTCCCTGTGAACCGTTAGATATCAAGCCTTTCCCTGAAGCGGACATCAAAAATATAGAGGATGTTGCAAGGAATTGCAAGTATCAGAAAGGACATTTCTTTTATGCTCCTTTTTGA", "aa": "MLSHSGEQLTQAFTGGTCDTPNLAELQKPIPPCEPLDIKPFPEADIKNIEDVARNCKYQKGHFFYAPF", "aa_hexdigest": "b967e35add83a4885812ff88f5d59c00", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7609.5613, "isoelectric_point": 5.107915687561035 }, "id": "NKOCPLAKPK_1628", "locus": "GCA003260355_01608" }, { "type": "cds", "sequence": "contig_1", "start": 861636, "stop": 861761, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGAAAATATTGATGTTTTGATGAAAGCTATTGAAGAAAATAAACCTACAGGAGTAAAAGGAAAACTCGTAAAGAAGGTTGTCGTAGCTACGACAATGAGTCCTGGTGTGCAAGTAGAATACTAG", "aa": "MENIDVLMKAIEENKPTGVKGKLVKKVVVATTMSPGVQVEY", "aa_hexdigest": "32cf379d43a74f405cffb0f2da4d3b59", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4475.295600000002, "isoelectric_point": 8.065011024475098 }, "id": "NKOCPLAKPK_1629", "locus": "GCA003260355_01609" }, { "type": "cds", "sequence": "contig_1", "start": 861860, "stop": 862390, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EJP7", "UniRef:UniRef90_A0A328EJP7" ], "nt": "ATGAAGCGAATCGGAGAAAATATGTTGAGAACGGTGCTAGGAAGAAAATTTATAAAGTCTAAAATTAGTTTTAGTGAGGTTTGGCAAGCTTTAAAAAAGGAGAAGAGAGGAACTTTTAAAACGGATGCAGAACCCCTCGGACATTTCGTGTCTTTAGATAATTTTGGTAGAGTGCCTGGAGGACTACTAGCAGTCGGTGATTATTTGTTTTCAGAAGATGGAGGAATTCTCAAAAAGGGAGATACGCACTGGCAGCCTTTTATAACGATTGATGTCCCAGATCGGGAACTGTTTCATAACTATGAGATGCTCAATGCGTTAGTTCTCACCTTTCGTAATCATACACTTTACTATACGCTTCCCAAAGATATGGAGAAAGAGAATCATTATCTTGCTCCTGAATATGAGCTTCAAAAAGATGGATCATGGAGATTAAATGCTTGTTTCCATCGAGAAAACCTTTGTCCAAAGAATGAAGAGTGTAAACTCAAGCAAAAGAAGCTTCCACTCAAGCAATGTAAAAACCTTTGA", "aa": "MKRIGENMLRTVLGRKFIKSKISFSEVWQALKKEKRGTFKTDAEPLGHFVSLDNFGRVPGGLLAVGDYLFSEDGGILKKGDTHWQPFITIDVPDRELFHNYEMLNALVLTFRNHTLYYTLPKDMEKENHYLAPEYELQKDGSWRLNACFHRENLCPKNEECKLKQKKLPLKQCKNL", "aa_hexdigest": "0e1c6cad81c1d3684b6ca779fb063636", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 861830, "stop": 862690, "observations": { "mutation": [ 861838, 861862, 862483 ], "stop": [ 861838, 861862, 862483 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EJP7", "query_cov": 1.0, "subject_cov": 0.5349544072948328, "identity": 1.0, "score": 365.0, "evalue": 9.01e-126, "gene_start": 1, "gene_end": 176, "reference_start": 144, "reference_end": 319, "reference_sequence": "MRKIFLSIMLVIGFLNFSLFAKDEDYQYFFPIPIDEFYGGTNDLHLSLQLACLKGEHLKEGSDFDPFWLPYCNLMKFVPSPYQQVDLSYYSGTTEEREKLHDTFSNIIGITIGLYNKASHKDQFHQQLQSFFHNWELSGSTQEMKRIGENMLRTVLGRKFIKSKISFSEVWQALKKEKRGTFKTDAEPLGHFVSLDNFGRVPGGLLAVGDYLFSEDGGILKKGDTHWQPFITIDVPDRELFHNYEMLNALVLTFRNHTLYYTLPKDMEKENHYLAPEYELQKDGSWRLNACFHRENLCPKNEECKLKQKKLPLKQCKNLGITLTTILGK" }, "paralog": false, "identity": 0.9895104895104895, "score": 589.0, "evalue": 2.19e-216, "description": "Internal stop codon at 861838, 861862, 862483. Nonsense mutation around 861838, 861862, 862483.", "psc": { "uniref90_id": "UniRef90_A0A328EJP7", "uniref50_id": "UniRef50_A0A328EJP7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EJP7", "UniRef:UniRef50_A0A328EJP7" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EJP7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EJP7" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 20576.572500000017, "isoelectric_point": 9.083547019958495 }, "id": "NKOCPLAKPK_1630", "locus": "GCA003260355_01610" }, { "type": "cds", "sequence": "contig_1", "start": 862481, "stop": 862597, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGTTTGTTCCTTCTCCGTATCAGCAGGTAGATCTCAGCTATTACTCTGGAACTACTGAAGAAAGGGAGAAGCTCCATGATACCTTTTCTAATATTATAGGTATTACTATTTGA", "aa": "MKFVPSPYQQVDLSYYSGTTEEREKLHDTFSNIIGITI", "aa_hexdigest": "701a0eff5e093c65c89f6e087a5d79f3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4408.891299999999, "isoelectric_point": 4.895849418640137 }, "id": "NKOCPLAKPK_1631", "locus": "GCA003260355_01611" }, { "type": "cds", "sequence": "contig_1", "start": 863255, "stop": 863932, "strand": "-", "frame": 3, "gene": "rplA", "product": "50S ribosomal protein L1", "db_xrefs": [ "GO:0003723", "GO:0003735", "GO:0006412", "GO:0015934", "KEGG:K02863", "SO:0001217", "UniParc:UPI000DBF5010", "UniRef:UniRef100_A0A328EI73", "UniRef:UniRef50_A0A288DFT5", "UniRef:UniRef90_A0A839JUL0" ], "nt": "ATGAAACACGGAAAAAAGTACAACGAAGTGAAAAAACTCGTTGAAAAAACAAAAGCCTATCCTTTGGCTGAGGCTGTAGAACTCCTCAAAAAGGTAAACTACGCAAAGTTTGACGCTGGAATTGAACTCGCAGTCAAGACTAATGCAAACCCAAAGTACAATGATCAAATGCTTAGAGCAACAACGATTCTTCCTCATGGAACAGGAAAAACCAAGAAAGTCGCAGTATTTACAACAGATGACAAGACAGAAGAAGCAAAGAAAGCGGGGGCGGACATCGCAGGATACGAATCTTTGCTCCACGACATCAAAAGTGGGAAGACAGATTTTGATGTGCTCATTACTACGCCTGATTTGATCAGAGAACTCGCTCCTGTAGCGAAACAGCTCGGACCTAAAGGACTTATGCCTTCTCCAAAAGCTGGAACAGTAACAGTAAATATCGCTCAAGCTGTGGAAGAGACGAAAAAGGGAAAGATTGAATTCAGACTTGATAAGACGGGAAACATCCATGCATTGGTAGGAAAGCTTTCCTTTGATAATGCAAAGCTCGTAGAAAATATTGATGCTTTGATGAAAGCTATTGAAGAAAATAAACCTACAGGAGTAAAAGGAAAACTCGTAAAGAAAGTTGTCGTAGCTACGACAATGAGTCCTGGTGTGCAAGTAGAATACTAG", "aa": "MKHGKKYNEVKKLVEKTKAYPLAEAVELLKKVNYAKFDAGIELAVKTNANPKYNDQMLRATTILPHGTGKTKKVAVFTTDDKTEEAKKAGADIAGYESLLHDIKSGKTDFDVLITTPDLIRELAPVAKQLGPKGLMPSPKAGTVTVNIAQAVEETKKGKIEFRLDKTGNIHALVGKLSFDNAKLVENIDALMKAIEENKPTGVKGKLVKKVVVATTMSPGVQVEY", "aa_hexdigest": "fcd6a3668dd2ac22bcf0b4a17e3a6568", "start_type": "ATG", "rbs_motif": "TTATT", "ups": { "uniparc_id": "UPI000DBF5010", "uniref100_id": "UniRef100_A0A328EI73", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBF5010", "UniRef:UniRef100_A0A328EI73" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EI73", "uniref90_id": "UniRef90_A0A839JUL0" }, "psc": { "uniref90_id": "UniRef90_A0A839JUL0", "gene": "rplA", "product": "50S ribosomal protein L1", "uniref50_id": "UniRef50_A0A288DFT5", "kegg_orthology_id": "K02863", "go_ids": [ "GO:0003723", "GO:0003735", "GO:0006412", "GO:0015934" ] }, "pscc": { "uniref50_id": "UniRef50_A0A288DFT5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A288DFT5" ], "product": "Large ribosomal subunit protein uL1" }, "genes": [ "rplA" ], "id": "NKOCPLAKPK_1632", "locus": "GCA003260355_01612" }, { "type": "cds", "sequence": "contig_2", "start": 534, "stop": 782, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCAAGCACAGACAGGAGATGATACTCCGATTGTTGTGATTACGGAAGCAAATAGCGGGGCAATTGTAACACAGACGGGAGATTCTCTCATCTATAGAAATGAAGTCTATGGATTTCAAGTAAAACTTGGGGAGGAGACTAAGGGATGTAAGCTCTTTGTGGATATAAATAGAGATAATATGCCTGATAATACTACTTTTCTTCTCTATGGAAAAATATGGACAGGAGAAGAGAGTCCTCTTGCCTGA", "aa": "MQAQTGDDTPIVVITEANSGAIVTQTGDSLIYRNEVYGFQVKLGEETKGCKLFVDINRDNMPDNTTFLLYGKIWTGEESPLA", "aa_hexdigest": "5f5d0e9b40be4edcbb6bfeb3493652f7", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9030.011699999997, "isoelectric_point": 4.273974037170411 }, "id": "NKOCPLAKPK_1633", "locus": "GCA003260355_01613" }, { "type": "cds", "sequence": "contig_2", "start": 1398, "stop": 1694, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MYA9", "UniRef:UniRef90_A0A660MYA9" ], "nt": "TTGGACTACGATGGACTTTCCGATGAGGTTATAATCTGGGCATTAAAGAGATACGAGGAGGAGATTGCTGAATATCGATCTTTCCCACGATGGAAACGTTGCTTTTTGCGGAATCCAGATCCTTTGCTAAGAAGAACCTATGATGATCTATTTTTCGATCCTCCCGTTTGGTATATGGAATTGAAAACAATAGAAGGAATCTACTCTATAGCTGGAGTGGGACTTGATGGAAGTTCCCTCTGCAAGTATATGGAGAAGACGGAGTATTTTATTGCTGTGGTATTGATCAAAAAGTAA", "aa": "MDYDGLSDEVIIWALKRYEEEIAEYRSFPRWKRCFLRNPDPLLRRTYDDLFFDPPVWYMELKTIEGIYSIAGVGLDGSSLCKYMEKTEYFIAVVLIKK", "aa_hexdigest": "34477c011e3505b376d02d1a09890c13", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 1398, "stop": 1773, "observations": { "insertion": [ 1722 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A660MYA9", "query_cov": 1.0, "subject_cov": 0.7903225806451613, "identity": 0.9590000000000001, "score": 204.0, "evalue": 1.63e-66, "gene_start": 1, "gene_end": 98, "reference_start": 27, "reference_end": 124, "reference_sequence": "MIHSITNKKYYKIMKTYSVKIEFSRPLDYDGLSDEVIIWALKRYEEEIAEYQSFPRWKRCFLRNPDPLLRRTYDDLFFDPPVWYMELKTIEGVYSIAGVGLDGSSLCKYMEKREYFIAVVLIKK" }, "paralog": false, "identity": 0.9596774193548387, "score": 245.0, "evalue": 3.9e-85, "description": "Frameshift due to insertion around 1722.", "psc": { "uniref90_id": "UniRef90_A0A660MYA9", "uniref50_id": "UniRef50_A0A660MYA9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A660MYA9", "UniRef:UniRef50_A0A660MYA9" ] }, "pscc": { "uniref50_id": "UniRef50_A0A660MYA9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MYA9" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 11731.441299999995, "isoelectric_point": 4.965192985534667 }, "id": "NKOCPLAKPK_1634", "locus": "GCA003260355_01614" }, { "type": "cds", "sequence": "contig_2", "start": 2026, "stop": 4224, "strand": "+", "frame": 1, "gene": "pflB", "product": "formate C-acetyltransferase", "db_xrefs": [ "EC:2.3.1.54", "GO:0005737", "GO:0005975", "GO:0008861", "KEGG:K00656", "SO:0001217", "UniParc:UPI000DBFA871", "UniRef:UniRef100_A0A328ECP1", "UniRef:UniRef50_P09373", "UniRef:UniRef90_A0A660MWF3" ], "nt": "ATGGCAATTGATGTAAGAAAGTTTGTCTATGACAACATCACTCCTTATGAAGGAGATGCATCTTTCCTTGCGGGACCTACAGAAAGAACCACTCAGCTCTGGGAAGAAGCCAAAAAATGTCTTAAAGAAGAAAGAGATGGGAATGGACTTAGATCTGCTGATCCTGAAACCATTTCTACCATTACTTCTCATAAACCTGGATATATCAAGCAAGAACTTGAAACCATCGTTGGTCTTCAGACAGATGAACCTTTGAAAAGGGCTATCAAGCCTTTTGGAGGAGTAAGAGTCGTAGAAAATGCCCTCAAAGAAAGAGGAATTGAACTTTCTGCGAGAGTAAAGGATATTTTCCATTATGCGAAAAATCATAATGATGCAGTATTTTCTGCTTATGATGCAGAATCTAGGCTTTATAGATCCAAGCATATCGTGACTGGTTTGCCAGACAACTATGCAAGAGGAAGAATTATCGGTGACTATAGAAGGCTTGCTTTGTATGGAACTGATGCTTTGATTAAAGAAAAGAAAGCTGACTTTGAAACGATTTCAGGAGAGATGACTGATGCAAAGGTAAGACTCAGAGAAGAAGTTTCAATGCAGATTCAGGCACTCAAAGAAATCGCTGAAATGGCTCAGTCTTATGGATTTGATGTAACAAGACCTGCAGAAACTGCTCAGGAAGCGATCCAATGGGTATACTTTGCTTATCTTGCTGCAGTAAAAGAGCAGGATGGAGCTGCGATGTCACTTGGTAATGTTTCTTCTTTCCTTGATATTTATATTGAAAGAGATCTTCAAGCAGGGAAAATTACCGAAGCTGAAGCTCAGGAGATGATTGACCATTTTGTGATGAAACTTAGACTTGTAAGACATCTTAGACCAGGAGCTTATGATGAAATCTTCGGAGGAGATCCTACTTGGGTAACTGAATCTATCGGAGGAATGTTCCATGATGGGAAAACAAAAGTAACAAAGACTTCATTCAGATTTTTACAGACTTTGTATAATCTTGGACCTTCTCCAGAGCCAAATCTAACAGTGCTTTGGTCAGATCACTTGCCAGAAGGATTCAAAAAATTCTGCGCTCAGGTTTCTATTGATACTTCATCTATTCAGTATGAAAACGATGACCTCATGAGGCCTATAAGAGGTGGAGATGACTATGGAATTGCCTGCTGTGTATCTTACCAAGAAATCGGAAAGAGAATCCAGCATTTCGGTGCGAGATGTAATCTTGGAAAGACTTTGCTCCTTGCGATCAATGGTGGAAAAGAAGAAGATGAAGGAGTGCAGGTAATGGACGCTTCACTTTTCGAAGCTGTAGAAAATGAAGATGGAACCTTGAACTATGATAAGGTGCTAGAAAACTTTAAAAAAGCAATGGCAAATGTTGCTAGAGTGTATGCAAAGACGATGTATGTAATCCACTATATGCATGATAAATACTACTACGAAAAAGCACAGATGGCGTTTGTGGATACCAATTATGGAGTAGATATTGCCTATGGTATCGCTGGAATCTCTATCGTTGCTGATTCCCTCTCTGCGATCAAATATGCGAAAACTAAAGCGATCAGAGACGAGCAAGGAGTAGCAAAAGATTTCCAAACTGAAGGAGAATTCCCAATGTATGGAAACGATGATGATAGAGTAGACCTTATCGCAAAAGAGATTCAGGCTTTCTTTATGGATGAATTGCTCAAGCATAGAGCCTACAAAGATGCAACTCCAACCCTTTCTCTCCTTACGATTACTTCTAATGTGATGTATGGAAAGAATACTGGAGCGACTCCAGATGGTAGAAAAGCGGGAGAGCCGTTTGCTCCAGGTGCGAACCCAATGCACGGAAGAGATCATTCAGGAGCAATTGCTTCGCTCAACTCTGTTGCAAAGCTTGACTATAAAAAGTCTCAAGATGGAATCTCAAATACCTTCTCTATCGTTCCAATGTCTCTTGGAGCGACTGCTGAGGAACAGAATGAAAATCTGGTTGGGATGATGAACGGATACTTCAAGAAAGGAGCTCATCACCTCAATGTGAATGTTCTCAAGAGAGAAACCCTTATGGATGCCTACGAACATCCAGAAAACTATCCTCAACTCACAATCAGAGTATCAGGATATGCGGTGAACTTTGTAAGGCTTTCAAAAGCGCATCAGCTTGAAGTGATTGAAAGAACCTTTCACCAGAGTATGTAG", "aa": "MAIDVRKFVYDNITPYEGDASFLAGPTERTTQLWEEAKKCLKEERDGNGLRSADPETISTITSHKPGYIKQELETIVGLQTDEPLKRAIKPFGGVRVVENALKERGIELSARVKDIFHYAKNHNDAVFSAYDAESRLYRSKHIVTGLPDNYARGRIIGDYRRLALYGTDALIKEKKADFETISGEMTDAKVRLREEVSMQIQALKEIAEMAQSYGFDVTRPAETAQEAIQWVYFAYLAAVKEQDGAAMSLGNVSSFLDIYIERDLQAGKITEAEAQEMIDHFVMKLRLVRHLRPGAYDEIFGGDPTWVTESIGGMFHDGKTKVTKTSFRFLQTLYNLGPSPEPNLTVLWSDHLPEGFKKFCAQVSIDTSSIQYENDDLMRPIRGGDDYGIACCVSYQEIGKRIQHFGARCNLGKTLLLAINGGKEEDEGVQVMDASLFEAVENEDGTLNYDKVLENFKKAMANVARVYAKTMYVIHYMHDKYYYEKAQMAFVDTNYGVDIAYGIAGISIVADSLSAIKYAKTKAIRDEQGVAKDFQTEGEFPMYGNDDDRVDLIAKEIQAFFMDELLKHRAYKDATPTLSLLTITSNVMYGKNTGATPDGRKAGEPFAPGANPMHGRDHSGAIASLNSVAKLDYKKSQDGISNTFSIVPMSLGATAEEQNENLVGMMNGYFKKGAHHLNVNVLKRETLMDAYEHPENYPQLTIRVSGYAVNFVRLSKAHQLEVIERTFHQSM", "aa_hexdigest": "f267fe63fada98323d69796b76d8dcc7", "start_type": "ATG", "rbs_motif": "TTTA", "ups": { "uniparc_id": "UPI000DBFA871", "uniref100_id": "UniRef100_A0A328ECP1", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBFA871", "UniRef:UniRef100_A0A328ECP1" ] }, "ips": { "uniref100_id": "UniRef100_A0A328ECP1", "uniref90_id": "UniRef90_A0A660MWF3" }, "psc": { "uniref90_id": "UniRef90_A0A660MWF3", "gene": "pflB", "product": "formate C-acetyltransferase", "ec_ids": [ "2.3.1.54" ], "uniref50_id": "UniRef50_P09373", "kegg_orthology_id": "K00656", "go_ids": [ "GO:0005737", "GO:0005975", "GO:0008861" ] }, "pscc": { "uniref50_id": "UniRef50_P09373", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P09373" ], "product": "Formate acetyltransferase 1" }, "genes": [ "pflB" ], "id": "NKOCPLAKPK_1635", "locus": "GCA003260355_01615" }, { "type": "cds", "sequence": "contig_2", "start": 4447, "stop": 4956, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MZY6", "UniRef:UniRef90_A0A328EGM0" ], "nt": "TTGATAGTAGGAGTAGGAAATGGATTTGCAGAAAATACGAGTAGAGATTCTCTATTCCAGGAGATAAAAAATGCTCGAAATTTTGCAAAAAACTGGGATACAATCCCAGGAAGTGATAACGATAAGCTTCTTGCTATCTTGACAGGGAAAACTATCCAGGTTGAAGATTCAAGAATAGCTGTCTGTACTCGCCGGTTTGGGCTTCTATTATTCAAAAAAGTTTATTATACTCGTCATATTTATTATGATGAGACAAATAAGCTTCTGAAAAAAGAGATCAAGTTCTCTCAAGAAAAATACGATCTCGTATGGAGTTTTCTAAGCTTGTGTGCCCAGTCATCATTATTACTTCTGCTATACATACTTACAAATCGTTTGTATTCGGACTATCGCACAACTTTTGACTGGATAGGATGGTGTATTGTTCTAGGCCTATTGATATATTTTTCTTACTACTATCTAGGGACTCTGTACGGCATTGTTTCTCTCCTTTATATTTTCAAAGATTAA", "aa": "MIVGVGNGFAENTSRDSLFQEIKNARNFAKNWDTIPGSDNDKLLAILTGKTIQVEDSRIAVCTRRFGLLLFKKVYYTRHIYYDETNKLLKKEIKFSQEKYDLVWSFLSLCAQSSLLLLLYILTNRLYSDYRTTFDWIGWCIVLGLLIYFSYYYLGTLYGIVSLLYIFKD", "aa_hexdigest": "75c29251f24b295dd5aa3e26080ec7e4", "start_type": "TTG", "rbs_motif": "TTATT", "psc": { "uniref90_id": "UniRef90_A0A328EGM0", "query_cov": 1.0, "subject_cov": 0.9388888888888889, "identity": 0.9940000000000001, "score": 333.0, "evalue": 1.66e-115, "valid": true, "uniref50_id": "UniRef50_A0A660MZY6" }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19805.748600000006, "isoelectric_point": 8.858745765686034 }, "id": "NKOCPLAKPK_1636", "locus": "GCA003260355_01616" }, { "type": "cds", "sequence": "contig_2", "start": 5743, "stop": 6003, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTTGATAGATTTGTAAAGCAGGGGATTGATGAGGAGGAATTTAAAAACGCAAAAAATTACCTCAAAGGAAGCATTCAAATGGGGATTGAAAGCTCTGACGAGATGGCAGATTTCCTGGCTTCTCAGTGGCTAGCTCAACAAAAAATTCTTACCTTGGATCAACTCTTAGAGAAATATGAAGCGATCAATATCGCACAAGTAAATGAACTTCTTCCTAAGCTCGCAGAATCTCAAAGATGGAGCTTCCATATCGAGTAG", "aa": "MLDRFVKQGIDEEEFKNAKNYLKGSIQMGIESSDEMADFLASQWLAQQKILTLDQLLEKYEAINIAQVNELLPKLAESQRWSFHIE", "aa_hexdigest": "30071436f42b9f9261b587d299ab07be", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10018.283399999995, "isoelectric_point": 4.698447608947753 }, "id": "NKOCPLAKPK_1637", "locus": "GCA003260355_01617" }, { "type": "cds", "sequence": "contig_2", "start": 6070, "stop": 6414, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF05193.26" ], "nt": "TTGCTGTTTACGATTGCAGGGAAGATTGAAAATCAGCTTGCTGTGGAGGGCCTCATCGCAGAACTTTTTTCCCAGCTCCCTCCAAAAAAAACAAGAAGGAAGCCAGATTTTCAGCGAGAATTACCAGAAAAGAAATCTGATTTTTTCAAAAAGGGTACAGAGCAAAATCATCTCATCCTCAGCATGCCGGGATTTTCTATTGATCAAGAGGAAAAATATGCCGCCAAAATCTTAACGACAATTTTGGGAGGAAATATGTCTTCAAGATTATTTCAAGAAATTAGGGAAGAATTAGGATTGTGCTACTATATTTCCGCAAGCCATCTTTCTAAAAAAGAATATTGA", "aa": "MLFTIAGKIENQLAVEGLIAELFSQLPPKKTRRKPDFQRELPEKKSDFFKKGTEQNHLILSMPGFSIDQEEKYAAKILTTILGGNMSSRLFQEIREELGLCYYISASHLSKKEY", "aa_hexdigest": "4c8cfec84a3366cd16673037955ac713", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF05193.26", "name": "Peptidase_M16_C", "length": 115, "aa_cov": 0.9736842105263158, "hmm_cov": 0.991304347826087, "evalue": 1.3749850835395844e-22, "score": 76.91166687011719, "start": 1, "stop": 111 } ], "seq_stats": { "molecular_weight": 13099.016999999989, "isoelectric_point": 8.66521167755127 }, "id": "NKOCPLAKPK_1638", "locus": "GCA003260355_01618" }, { "type": "cds", "sequence": "contig_2", "start": 6520, "stop": 6762, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATAAAATTGGCGCTGAATTTAATGCAGGTACTGGAGGAAGTCAGACTTCTTACTATATAAAGTGTGCTCCTCAATTTGCTGAAGTTGCACTAAGGCTCTTGGCTGATATGATGTTAAATGCACAAATAAATCCCGAAGAACTACACAAAGAAAAAGAGGTAATTATTCAAGAACTCAAAATGTATCAAGATAATCCAATGGCTACAGTGCGCGAAAAGCGAGCATTATTTTTCGTGTGA", "aa": "MDKIGAEFNAGTGGSQTSYYIKCAPQFAEVALRLLADMMLNAQINPEELHKEKEVIIQELKMYQDNPMATVREKRALFFV", "aa_hexdigest": "b10ab3c138e8904058de82787fd4088a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9096.447199999993, "isoelectric_point": 5.2908235549926745 }, "id": "NKOCPLAKPK_1639", "locus": "GCA003260355_01619" }, { "type": "cds", "sequence": "contig_2", "start": 6799, "stop": 6915, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAGAGCTCAATTCTCTTACCATACAAATCTCAGTAAAAGCAGGATCAGAATATGAAACAAGAGAAGAAGCAGGTATTTCTCATATTTTGGAGCATATGTTTTTTAAGGGATGA", "aa": "MQELNSLTIQISVKAGSEYETREEAGISHILEHMFFKG", "aa_hexdigest": "37103dc0ed0f2507071af23f726b1af7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4324.8429, "isoelectric_point": 5.0550554275512685 }, "id": "NKOCPLAKPK_1640", "locus": "GCA003260355_01620" }, { "type": "tRNA", "sequence": "contig_2", "start": 7086, "stop": 7159, "strand": "-", "gene": "trnC", "product": "tRNA-Cys(gca)", "amino_acid": "Cys", "anti_codon": "gca", "score": 55.3, "nt": "GGCGCCCTGACAGAGTGGTCTATTGTACAGGTCTGCAAAACCTCGGTTCATGGGTTCGAATCCCATGGGCGCCT", "db_xrefs": [ "SO:0000258" ], "anti_codon_pos": [ 7123, 7125 ], "id": "NKOCPLAKPK_36", "locus": "GCA003260355_01621" }, { "type": "cds", "sequence": "contig_2", "start": 7372, "stop": 7710, "strand": "-", "frame": 2, "gene": null, "product": "50S ribosomal protein L28", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBFAF8A", "UniRef:UniRef100_A0A660MUM9", "UniRef:UniRef50_A0A288GTB0", "UniRef:UniRef90_A0A7W4HH14" ], "nt": "ATGGCGAGTAGGACAAATAAACAGGGAATTGCAAAGAAGAAAGCCCAGCAGGCAAAGCAAGCTAGTAGAGTGCGTGGGAGTAGGAGATTTGACTATCCAAAAAGAAAACTAGAGCTTGAAGAAGCAAAAGAGCTCGGAATTGTTGGGATTTATACCAATCCTAAAGGTGCAACCTACAACCGATTTCTTAGAGGGAGAAAAGTCTGTTGGGAGACGAGGTTGAGAACAGATCCTGATGGATACAGATATATAAGGACGCCAGCAGGGGGTTCAGAAATGGTTTCAGCACCGGCATCAGGATGGACGAGAGGTAAAATGAGGAAAATGATGAGAAAATAA", "aa": "MASRTNKQGIAKKKAQQAKQASRVRGSRRFDYPKRKLELEEAKELGIVGIYTNPKGATYNRFLRGRKVCWETRLRTDPDGYRYIRTPAGGSEMVSAPASGWTRGKMRKMMRK", "aa_hexdigest": "f889096402b83e8138d5baae6b3b9ea5", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000DBFAF8A", "uniref100_id": "UniRef100_A0A660MUM9", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBFAF8A", "UniRef:UniRef100_A0A660MUM9" ] }, "ips": { "uniref100_id": "UniRef100_A0A660MUM9", "uniref90_id": "UniRef90_A0A7W4HH14" }, "psc": { "uniref90_id": "UniRef90_A0A7W4HH14", "uniref50_id": "UniRef50_A0A288GTB0" }, "pscc": { "uniref50_id": "UniRef50_A0A288GTB0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A288GTB0" ], "product": "50S ribosomal protein L28" }, "genes": [], "id": "NKOCPLAKPK_1641", "locus": "GCA003260355_01622" }, { "type": "cds", "sequence": "contig_2", "start": 7788, "stop": 8450, "strand": "-", "frame": 3, "gene": null, "product": "Membrane insertase YidC/Oxa/ALB C-terminal domain-containing protein", "db_xrefs": [ "KEGG:K03217", "SO:0001217", "UniRef:UniRef50_A0A2W4IL92", "UniRef:UniRef90_A0A328EBX6" ], "nt": "TTGAGAAATGCTATAGCTGGAGCAAATATGAGCAAAGGGATGTCCAATATCCAACCAAAAATGCAGGAACTTCAAGAGAAATACAAAGATGATCCTAAAAAGCTTTCTGAAGAGACCATGAAACTACTCAAAAAAGAAGGTGCTGGTCCGATGAAAGGATGCTTATCTATGCTTATCCAGCTTCCAGTATTCATTGGGCTTTATTCTGTAATTAGAAAATTTGCTACGGGAGAAGTGCCAAATGAATGGCTTTATAGCTTCTTCTTCGGCTTTGGTAATCAGTATCTAGATACACAAAATATTCATTCAATGTTTTTGGGGATTGACCTTTTTGAAAAAAATAGTTTAATTCTGACTATTCTCGTTGCTTGTCTAAATGCACTACAGTTCTGGCTTACAAGTTTGGTCCAAAAAACCAACAAACCGGCTGCAATGCCTGGTGGTGTCCCAATGCCAGATATGTCAAAAATGATGATTCCAATGAACATCATGATGACGGTGATGATGGCTTCTTTTGTTTGGTCTTTCCAAGCAGGTGTTGGACTTTATCTCGCTACGACCTCACTTTTCAGTGTGCTGCAGTATACGATACAGTATCGTGTCCTTCTCAAAGCAAAATTGGATATGCTTTTGCACAAGAATAAGCCCGTAGTGATCAATTAG", "aa": "MRNAIAGANMSKGMSNIQPKMQELQEKYKDDPKKLSEETMKLLKKEGAGPMKGCLSMLIQLPVFIGLYSVIRKFATGEVPNEWLYSFFFGFGNQYLDTQNIHSMFLGIDLFEKNSLILTILVACLNALQFWLTSLVQKTNKPAAMPGGVPMPDMSKMMIPMNIMMTVMMASFVWSFQAGVGLYLATTSLFSVLQYTIQYRVLLKAKLDMLLHKNKPVVIN", "aa_hexdigest": "ad02d9e31b4ad0bb1004ed27044c131d", "start_type": "TTG", "rbs_motif": "TTATT", "psc": { "uniref90_id": "UniRef90_A0A328EBX6", "query_cov": 1.0, "subject_cov": 0.8270676691729323, "identity": 0.991, "score": 418.0, "evalue": 8.22e-147, "valid": true, "product": "Membrane insertase YidC/Oxa/ALB C-terminal domain-containing protein", "uniref50_id": "UniRef50_A0A2W4IL92", "kegg_orthology_id": "K03217" }, "pscc": { "uniref50_id": "UniRef50_A0A2W4IL92", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4IL92" ], "product": "Membrane insertase YidC/Oxa/ALB C-terminal domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_1642", "locus": "GCA003260355_01623" }, { "type": "cds", "sequence": "contig_2", "start": 8652, "stop": 9200, "strand": "-", "frame": 3, "gene": null, "product": "Ribosome recycling factor", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBF731D", "UniRef:UniRef100_A0A660MTS4", "UniRef:UniRef50_A0A288DBT7", "UniRef:UniRef90_A0A660MTS4" ], "nt": "ATGGAGCTTTCGGTTTATGATCAACAAATGAAAAAGTCAATCGCCTACCTTGAGCAAGAATTTGCAGGTTTACAGGTTGGAAGAGCGACAACAGGGCTTGTTGATAATTTAAATGTGGAGACGGAGTATGGGGTAATGAAGCTTAATACCTTAGCTCATATTACCGTAATGGATACTACAACATTGAAGATTGAGCCTCGAGATAAAGCAAGCTCAAAACATATTGCGAATGCAATTTACGAAGCAGATTTGGGAGTAGGATTAGATAATCAGGGTTCTCATATTTTGGTGAAAATTCCTGCTTTGACTCAAGAAAGAAGAGATCATATCGCTAAGCAGGTAAAGGCAATGGGAGAAGATACTAAAACGCAAATCAGAGGGATCAGGCAGGATGCGATGACTGAGACTAAAAAGCAGTTTACTGCCAAGGAAATCTCAGAAGACGTCCACAAGGCAAATGAAAAAAATATTGATGAACTTACCAAAATCCAATCTGCCAATGTTGATAACTTGGTAAAAGCGAAAGTAAATGAAGTGATGAAGGTATAG", "aa": "MELSVYDQQMKKSIAYLEQEFAGLQVGRATTGLVDNLNVETEYGVMKLNTLAHITVMDTTTLKIEPRDKASSKHIANAIYEADLGVGLDNQGSHILVKIPALTQERRDHIAKQVKAMGEDTKTQIRGIRQDAMTETKKQFTAKEISEDVHKANEKNIDELTKIQSANVDNLVKAKVNEVMKV", "aa_hexdigest": "7b71155d9bf764d2e1d5a2da22f0eec4", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000DBF731D", "uniref100_id": "UniRef100_A0A660MTS4", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBF731D", "UniRef:UniRef100_A0A660MTS4" ] }, "ips": { "uniref100_id": "UniRef100_A0A660MTS4", "uniref90_id": "UniRef90_A0A660MTS4" }, "psc": { "uniref90_id": "UniRef90_A0A660MTS4", "product": "Ribosome recycling factor", "uniref50_id": "UniRef50_A0A288DBT7" }, "pscc": { "uniref50_id": "UniRef50_A0A288DBT7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A288DBT7" ], "product": "Ribosome recycling factor domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_1643", "locus": "GCA003260355_01624" }, { "type": "cds", "sequence": "contig_2", "start": 9403, "stop": 9789, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTTTAAGCACGATAGTACTGTTTTCTCAGCCAAGTTTTGCTCAGCAAAAAACTTCCACCTGTGGAGATTCCCCCGCTGTCTGCTCTGCGACACCCGAAAGAATGGTACTTTATCTCGATTTTCAGGAAGAAATATCAGAACTTATCGAAACAAATCCTTTTGAGACCGTAGTTGGAGCGATTAGTAAGGGGGAAGGAGGAATTTTTACGAATGAGCTTTTACAACTGAGTGGGATTCAGAATTTTGATGATAGTTTAGCTGGGCAAAGCCTTAAATTAGCTTATATTGCCAATACAAGATCAGCGCTCTCCCTTCTTACCAGCATCTACCTCTTTGAACTTTCTGCGATTACGACCGTTCAAGATGTGACGCTGGGGCTTTGA", "aa": "MILSTIVLFSQPSFAQQKTSTCGDSPAVCSATPERMVLYLDFQEEISELIETNPFETVVGAISKGEGGIFTNELLQLSGIQNFDDSLAGQSLKLAYIANTRSALSLLTSIYLFELSAITTVQDVTLGL", "aa_hexdigest": "81d2c72a01dcfc07fdf3e80989f9322b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13788.532999999978, "isoelectric_point": 4.073332405090333 }, "id": "NKOCPLAKPK_1644", "locus": "GCA003260355_01625" }, { "type": "cds", "sequence": "contig_2", "start": 9826, "stop": 10167, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATTGCTAGATATTGAGCGAAATCTTAGCAAGACCCTTTATTATCTTGGGAAAACTGAGAACATCATGAGGACTATTTCTCAGACTGAGAAACTCAGAAATCTCTTAAAAACCTATGAAGAAAAAGGTCTTTTTGTGCAGACTGCGAATTTTCCTAGTCAAATCAAATATCTTGATCTCCTAAAAATGTTGATTAGCCTTAATATAGCGATGAAGGATTTTCTAGCCTATAATAGCGTAGATCCACTCAGGAAGTTTTCTACTCATCAAATAAAGTTTAACGAAAAATGGATTAATCAGCTTGATGACGACTATCATTGTGTTAGATGGGATCTTTGA", "aa": "MKLLDIERNLSKTLYYLGKTENIMRTISQTEKLRNLLKTYEEKGLFVQTANFPSQIKYLDLLKMLISLNIAMKDFLAYNSVDPLRKFSTHQIKFNEKWINQLDDDYHCVRWDL", "aa_hexdigest": "d9f76898979d49b1fc9c9827d0507f89", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13566.643299999985, "isoelectric_point": 8.96718158721924 }, "id": "NKOCPLAKPK_1645", "locus": "GCA003260355_01626" }, { "type": "cds", "sequence": "contig_2", "start": 10762, "stop": 10863, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTATGATTAAAAATCTCCAAGACTTAAGCAATTCTAGACAAGAAGCTGAGCAGCTTTCTGACATTGCGAATAATTTTGCACTTACGTATCAGTTTTGA", "aa": "MLMIKNLQDLSNSRQEAEQLSDIANNFALTYQF", "aa_hexdigest": "dc761a7d343383c8cfd183a7e16fadcf", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3847.2887999999994, "isoelectric_point": 4.316091728210449 }, "id": "NKOCPLAKPK_1646", "locus": "GCA003260355_01627" }, { "type": "cds", "sequence": "contig_2", "start": 11021, "stop": 11203, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTATCACTATTTATGAACTCTCCTCTTTGGAAGCAGATATTATTTGGGATAATGGGGCTACTTGTGGGTTTTGCTCCAGCTTCGGCTCAGCAAATTAATAGAGACGAGAATGACGTAGTTCCAGAGCTGGGGACCTCACTTATCTCTGCTGCCGTCACCAACCTCAATACAAAACTCTGA", "aa": "MLSLFMNSPLWKQILFGIMGLLVGFAPASAQQINRDENDVVPELGTSLISAAVTNLNTKL", "aa_hexdigest": "54018637767a82ca658df7f4bb7351dc", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6489.537200000001, "isoelectric_point": 4.780523109436035 }, "id": "NKOCPLAKPK_1647", "locus": "GCA003260355_01628" }, { "type": "cds", "sequence": "contig_2", "start": 11255, "stop": 11545, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTGCAAATCCCCAGTTTGCTACCGGTTTTTCTTCTAGTATTGAAAGTTATGATAGCAGCCAATCACTCTTTTTGAGCATTCTTTGTAATACCAACGGACTGGAAAACAAGTATTTTAACCAGCAGACTCAAAAGTACCTCAAAGAGCTCTCTTACAAGAAGTTAAAGTTTGATAAAGCCTGCCAAAGAAGCTATACTGAAAAGTGTAATGTTGCGGAGCTGGCTGATGACCTCCTTACTCAACTATTTTCTGAGCTATTTACTTTTAAACAGGCGGAAATTTTTTGA", "aa": "MAANPQFATGFSSSIESYDSSQSLFLSILCNTNGLENKYFNQQTQKYLKELSYKKLKFDKACQRSYTEKCNVAELADDLLTQLFSELFTFKQAEIF", "aa_hexdigest": "add022cefed5aa5542dde74b62311909", "start_type": "ATG", "rbs_motif": "TTATT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11052.33519999999, "isoelectric_point": 5.3342485427856445 }, "id": "NKOCPLAKPK_1648", "locus": "GCA003260355_01629" }, { "type": "cds", "sequence": "contig_2", "start": 11579, "stop": 11845, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATAAAAAAATTGATGCCTATGCTGAAGAGAAATATAAGATTCCCCACTTCTGTACTGATAGAATCCATAATTATAGCCAGACTTGCTCTATTATAAGCAGACAAATGAAAGCTTTTGTTCCTGTAATTAAAGGATTTAAGATTCTCAACTCCGATGAACTCTATAAAAACGTAAAGGATAAAGAGTTAACTTTCCCTCCCTGTGAGATTGGACCTCACTATGATCTTGTCCTTTGTGGAACTCTAGGAGAGACGGATAAATGA", "aa": "MNKKIDAYAEEKYKIPHFCTDRIHNYSQTCSIISRQMKAFVPVIKGFKILNSDELYKNVKDKELTFPPCEIGPHYDLVLCGTLGETDK", "aa_hexdigest": "a2e770fb5bd27fd5d09fab159870c6d0", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10170.675899999993, "isoelectric_point": 7.658451652526856 }, "id": "NKOCPLAKPK_1649", "locus": "GCA003260355_01630" }, { "type": "cds", "sequence": "contig_2", "start": 11864, "stop": 12178, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTCTATAACGAACTTGCACGATATACGATGTTTTCAACATATTATACACAAGCACTTATGCAAAGAAGCAGAATAGAGCTTCCTACTCAAGATGAAATTAGTCTTTTGAAAGAAAATCGAGGAAAATTCTTTTTCTTGACCAATCAAACGCTAAACGACCTCACAAACCTTGCTACAACCTACCCTATTCATATTGGATTTTTGGCATATCAAGAGGATCTTCTTCGCTTGAGAGACTCCTCTCTTAGCAAGCTAGTTACCCCATTTTATACTCTCTATCATAAACTCAGAAATGTCCAGTCCGAAACTTAA", "aa": "MLYNELARYTMFSTYYTQALMQRSRIELPTQDEISLLKENRGKFFFLTNQTLNDLTNLATTYPIHIGFLAYQEDLLRLRDSSLSKLVTPFYTLYHKLRNVQSET", "aa_hexdigest": "16b65df3a4a1e02900ada32e01d5bcc9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12369.000699999984, "isoelectric_point": 7.985263633728028 }, "id": "NKOCPLAKPK_1650", "locus": "GCA003260355_01631" }, { "type": "cds", "sequence": "contig_2", "start": 12159, "stop": 12311, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCCAGTCCGAAACTTAAATCTCATCCTTCCATTCGCTATTTTTTCTCTGGTTTGAGCCTGTTTTTATTGTTTTTTGCTATTATTTGGGATGCTAGTGCTTCTGCGACAACCTGGAAGATTGAAGGAGCAGGGCGTGCACAGCTTTTTTGA", "aa": "MSSPKLKSHPSIRYFFSGLSLFLLFFAIIWDASASATTWKIEGAGRAQLF", "aa_hexdigest": "061a5dfce2c8431c649a880a7210bc55", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5596.4592, "isoelectric_point": 9.992035484313966 }, "id": "NKOCPLAKPK_1651", "locus": "GCA003260355_01632" }, { "type": "cds", "sequence": "contig_2", "start": 12420, "stop": 12698, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTCTTCTGTCATTAATGGGCTTGAATCTACAAAAAAGATTCTGAATAGTCAATATGCTAATTGTGATCTCTCTGATGACAATATTTCCTCTATTTTATTTTTTTCTAATACAGGATTTTCGAAAGAGCTTAGGCGTGCTCTCCCAGAATCAAAATATCCTACAAGGAAAGAATATGAAAAAACCTGTGAAAAGCTTACTTCTTGCATAGAGGGGAAAGCTGACACGAATCTTAATAAAGCCTGTGATGATCTCATCAATACTACGTATGCTTTTTGA", "aa": "MSSVINGLESTKKILNSQYANCDLSDDNISSILFFSNTGFSKELRRALPESKYPTRKEYEKTCEKLTSCIEGKADTNLNKACDDLINTTYAF", "aa_hexdigest": "21f46d73232c7fe84df004ded08ec274", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10344.501399999986, "isoelectric_point": 5.377218818664551 }, "id": "NKOCPLAKPK_1652", "locus": "GCA003260355_01633" }, { "type": "cds", "sequence": "contig_2", "start": 12702, "stop": 12890, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGACGGAAGGAAATCAACTTACCATTGAGGAAAGCAATCTTGGAAAAGATAGGTATCAAAATGGGAATTCTGAAGACTCTGCTTATGACTTATTGGAAGATATCCGCCAGATTGGAACTATTTTTTTTCAAGAGACCAAGGAAGCTCCTGAAACGGTTTTTTATAGGCTTCCTGATTTTAAGGTATGA", "aa": "MTEGNQLTIEESNLGKDRYQNGNSEDSAYDLLEDIRQIGTIFFQETKEAPETVFYRLPDFKV", "aa_hexdigest": "97da1e64cab711de2871c4f8b2660429", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7205.799499999998, "isoelectric_point": 4.250556373596192 }, "id": "NKOCPLAKPK_1653", "locus": "GCA003260355_01634" }, { "type": "cds", "sequence": "contig_2", "start": 12977, "stop": 13123, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGGCATTAATAATAGAATCTTCACTGAGAGGGAGAATATCATCTGAAGGACTTTCTTCATCCGATCCTTGTCTAAGACGACTCAGAGAGCGAGCAGAATTAGTAGAAGTGGTAGTATTAGAAGAAACAGGAGAAGAATGCTGA", "aa": "MKALIIESSLRGRISSEGLSSSDPCLRRLRERAELVEVVVLEETGEEC", "aa_hexdigest": "b9a9d205638cfd315a9195d0187aebcf", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5347.066899999998, "isoelectric_point": 4.795187568664551 }, "id": "NKOCPLAKPK_1654", "locus": "GCA003260355_01635" }, { "type": "cds", "sequence": "contig_2", "start": 13167, "stop": 13538, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGCTTTTGTAAATCACTGTACAATCTCTGGAAGTCCACTTATTAAGCAGGCAGAGCAAGCGGCTCATCAGCTCGAAAATAATACAAAAAGTACATCATCCTCAAAACAGCAATCTTCCTCCGTGGCGAGTTCTCCCACTCAAGTTCTTCCGAGTGCTAATGCACTTATGCTTTCTCCGCAAGAGATCAATAGTTATATCACAGATATCCTGAGAAATAGTAATAGCATCAAATCTGCTGTTTCTCCTACTCTTCGTAGAGGAGAGACAGAAAACAACACAACCTCTTTGGTTGCATCTTCGGATTCTGCTATTATTGCTGATGTCAAAAAACAGCTGGAATCTTGTAGTAATAAATGTGATGAGCTATGA", "aa": "MAFVNHCTISGSPLIKQAEQAAHQLENNTKSTSSSKQQSSSVASSPTQVLPSANALMLSPQEINSYITDILRNSNSIKSAVSPTLRRGETENNTTSLVASSDSAIIADVKKQLESCSNKCDEL", "aa_hexdigest": "532401107d4723bdf8521f4a903e416a", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13083.380599999982, "isoelectric_point": 6.065595817565917 }, "id": "NKOCPLAKPK_1655", "locus": "GCA003260355_01636" }, { "type": "cds", "sequence": "contig_2", "start": 13626, "stop": 13835, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAAGAAGGAGCCTTTAAACTAAGGTTCTGTACCGTTCCAAGCAAAATAACTCTTGCAAATAGTAATGTCAAAATCGTTTATAGTATTGAAGAAATCTTGGGAGAAATCCAGAAAGCTATTAATGGTCTGTATGATAGCGGAGAACTGACCACGAAGACGAGAAATAAAGAATTTCTCAATACTTCTCAATCAAAATTCAAATTCTGA", "aa": "MEEGAFKLRFCTVPSKITLANSNVKIVYSIEEILGEIQKAINGLYDSGELTTKTRNKEFLNTSQSKFKF", "aa_hexdigest": "e9fb774537db30cca532413843c5e546", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7820.901699999997, "isoelectric_point": 8.818066215515138 }, "id": "NKOCPLAKPK_1656", "locus": "GCA003260355_01637" }, { "type": "cds", "sequence": "contig_2", "start": 13851, "stop": 14222, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGCTGGTGTTGGAAGGAAGGCTCCGACTACAACAACCTATCAAAAACAGCTCCAAAAGACAGAACAAGAGCTTAATAAATCCACTAAAGATAGTATGGTTCCAGATAGTGATGAGAGGAATCGCTACCTTCTTTTGGAGGATTATACTATCACCAAGAGGGAAAGAAGCATTGAGAAACCGGAAGTTGGGCTTCCAAAACCAGCAGATCAAGCGCTTTCTACTGAGGCTCTTGAATCACAGAAAAAATATGCGTCTATCAACTTTCTTGTAAGCAATTTCCTTGATCAGAATGCAATTCTTCTGATCAACCTCAATCAAACTCTCTTGGAGATGAATGAGGCACTTAATTATCTTTTACAGAAGAAATAG", "aa": "MAGVGRKAPTTTTYQKQLQKTEQELNKSTKDSMVPDSDERNRYLLLEDYTITKRERSIEKPEVGLPKPADQALSTEALESQKKYASINFLVSNFLDQNAILLINLNQTLLEMNEALNYLLQKK", "aa_hexdigest": "61b806eef53a373dab86750bcb452667", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14063.836899999982, "isoelectric_point": 6.325065803527832 }, "id": "NKOCPLAKPK_1657", "locus": "GCA003260355_01638" }, { "type": "cds", "sequence": "contig_2", "start": 14574, "stop": 15743, "strand": "-", "frame": 3, "gene": null, "product": "DUF4012 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MRS3", "UniRef:UniRef90_A0A660MRS3" ], "nt": "GTGCGTTACGGACAATATGGCGGTATTCTTGATGATCGTTTCTCCGAGATTTTCCATGATTGTGGAAGCGAATATTACAACCAATTCACTACATTGAGGGATTTTTCTGCATTATCAAGAACCTTAGAATTAGGGTATGCCGATGCAAAAATCTATTCAAGTCAGATTCTCAATGAATACAAACTAATTTCCTTACAACAAGCAATTTATAAGCAACTTTCAAATACTTCCGATGTAAAATCCTTGATGCAGTCATACTTTAACTTCCTTCGTGAAATTCTTATTAAGGAAAAATCAAAAAATGCTGATCTCATCGGAGCATTCCCAAAGACTTTCACGTATCGATACAATATGAATATTATCGCTCCTTATCTCAAGGATGAAAACTCAAAAATGACGAAAGAAGACAGAACGGCATTAACCAGTCAGCTTCTCTCTCTTAATTATGGAGAAAAGATTGCTAATTTTGCTGGTTTACAAGACCAGATCCGTTACGAAATCTCTTCATCAACAGGGGCTCCTCATCAACAGTCGATTAACTCAGAAAAAGAAGAACAGGACTTAGAGAGATTATTTAGAAGTAGTTATCTTCCTGCTCAATTTACGCTAATTTCAGTGAAGAAAGGCAAAGAGTCTTCAACGCTCCTCGTTCAAGGGGTAGATAGAAGGACAAATCTTACAGTAGAGGCAAAGCTTAAGTATGAGAACATTCTCTTGTCAGTTACTGAGATTAAAGTTGCAAAGAATCCAAAGCTGACTGAATATCTCAACGCATTGATTAAAAATGATAGACTCTCACTCAATAAGGTGCTAAATTTGATGCAGGAAAACAAACAAATCGCAGAGCAAACACAGCCTTTAGACTTGAGACTTTGTTCTCAGCTCCAAGGAAAATATGGAAGAGAACTTCTTTCTTGTTCTAATACGCAAGCAAAAATCTCTCTTGCTACAGGAACAAGGCTTTCTGATGGCACAGTTACCGCGAGCCCATTAGGATATACCTTAGGGCTAAAAAATGGTGCACTACACAAGCTAGAAATCTCAGATAAAGTCCTTGAGACCAAGATATTAGAAGAAATAGATTTTTCACTTGTTGATGCTTCATCGACATTCTATATCATAAATGCTCTCATTGGTTATGTTCCAAAAGAAAAAAATTCTGGTTTCTGA", "aa": "MRYGQYGGILDDRFSEIFHDCGSEYYNQFTTLRDFSALSRTLELGYADAKIYSSQILNEYKLISLQQAIYKQLSNTSDVKSLMQSYFNFLREILIKEKSKNADLIGAFPKTFTYRYNMNIIAPYLKDENSKMTKEDRTALTSQLLSLNYGEKIANFAGLQDQIRYEISSSTGAPHQQSINSEKEEQDLERLFRSSYLPAQFTLISVKKGKESSTLLVQGVDRRTNLTVEAKLKYENILLSVTEIKVAKNPKLTEYLNALIKNDRLSLNKVLNLMQENKQIAEQTQPLDLRLCSQLQGKYGRELLSCSNTQAKISLATGTRLSDGTVTASPLGYTLGLKNGALHKLEISDKVLETKILEEIDFSLVDASSTFYIINALIGYVPKEKNSGF", "aa_hexdigest": "82e4444890a4da216ec7936fd88df686", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 14271, "stop": 16043, "observations": { "mutation": [ 14495, 14498, 14576, 15764, 16010 ], "stop": [ 14495, 14498, 14576, 15764, 16010 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A660MRS3", "query_cov": 1.0, "subject_cov": 0.510498687664042, "identity": 0.987, "score": 736.0, "evalue": 6.62e-262, "gene_start": 1, "gene_end": 389, "reference_start": 260, "reference_end": 648, "reference_sequence": "MKESIFFNKEQLKIIHWYGIVYKGVFRYGVTTISLILSFVALGYFVCSSCFSPKFIAEEALPSSTESQDFYVDKQQTVFAISGLTAKILNGKIESNTEGFNSENNLLEMEGLVLPELTSLSFENLDTFVEHQNDEVFNDAYLKKFFSSVIEAPLKQQFSFKETAKLLPLSGSIHESFGLNCVEGATKYSFVCKSYIKNFLDQFFLYNLDDVHLKTEENSEEGSEDPNTKGGMKISEELTNLYKLLRKNKNYRGKFCQGLVRYGQYGGILDDRFSEIFHDCGSEYYNQFTTLRDFSALSRTLELGYADAKIYSSQILNEYKLISLQQAIYKQLSNTSDVKSLMQSYFNFLREILIKEKSKNADLIGAFPKTFTYRYNMNIIAPYLKDENSKMTKEDRTALTSQLLSLNYGDKIANFAGLQDQIRYETSSSTGTPHQQSINSEKEEQDLERLFRSSYLPAQFTLISVKKGKESSTLLVQGVDRRTNLTVEAKLKYENILLSVTEIKVAKNPKLTEYLNALIKNDRLSLNKVLNLMQENKQIAEQTQPLDLRLCSQLQGKYGRELLSCSNTQAKISLATGTRLSDGTVIASPLGYTLGLKNGALHKLEISDKVLETKILEEIDFSLVDASSTFYIINALIGYVPKEKNSGFGMKEHLLVADKFSKYLDIIPEKVISEGGTVKVYFAVGELHFIGTYDLVKNVLRPIAIDFGTKRRPVVVQDFELVFRDDKAETLNSFLLDPLAFLRELNAALVEKYFDKPASANK" }, "paralog": false, "identity": 0.9830508474576272, "score": 1127.0, "evalue": 0.0, "description": "Internal stop codon at 14495, 14498, 14576, 15764, 16010. Nonsense mutation around 14495, 14498, 14576, 15764, 16010.", "psc": { "uniref90_id": "UniRef90_A0A660MRS3", "product": "DUF4012 domain-containing protein", "uniref50_id": "UniRef50_A0A660MRS3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A660MRS3", "UniRef:UniRef50_A0A660MRS3" ] }, "pscc": { "uniref50_id": "UniRef50_A0A660MRS3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MRS3" ], "product": "DUF4012 domain-containing protein" } }, "id": "NKOCPLAKPK_1658", "locus": "GCA003260355_01639" }, { "type": "cds", "sequence": "contig_2", "start": 15762, "stop": 15890, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCATCTAAAAACAGAAGAAAATTCTGAAGAAGGCAGCGAAGATCCAAATACAAAAGGAGGGATGAAAATTTCAGAAGAGTTAACAAACCTTTATAAGCTTCTAAGAAAAAATAAAAATTATCGCTGA", "aa": "MHLKTEENSEEGSEDPNTKGGMKISEELTNLYKLLRKNKNYR", "aa_hexdigest": "d3c59f7950af1f7082e9420116b3428f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4926.4549000000015, "isoelectric_point": 6.556855201721191 }, "id": "NKOCPLAKPK_1659", "locus": "GCA003260355_01640" }, { "type": "cds", "sequence": "contig_2", "start": 16008, "stop": 16307, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGATAAGCAGCAGACCGTTTTTGCTATCTCCGGGTTAACGGCAAAGATTTTAAACGGGAAAATAGAATCAAGTACTGAAGGATTTAACTCAGAAAATAACCTCCTCGAAATGGAAGGGTTGGTTCTCCCAGAGTTGACTTCTCTTTCATTTGAAAACCTTGATACCTTCGTTGAGCACCAAAATGACGAAGCTTTCAACGATGCTTATCTAAAGAAGTTTTTTGCATCAGTAATAGAAGCTCCCTTAAAGCAGCAGTTTTCATTTAAAGAAACTGCCAAACTTCTTCCCTTAAGCTGA", "aa": "MDKQQTVFAISGLTAKILNGKIESSTEGFNSENNLLEMEGLVLPELTSLSFENLDTFVEHQNDEAFNDAYLKKFFASVIEAPLKQQFSFKETAKLLPLS", "aa_hexdigest": "c02bfce628bb742a9b4d685d900cf0fd", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11091.406199999985, "isoelectric_point": 4.535660743713379 }, "id": "NKOCPLAKPK_1660", "locus": "GCA003260355_01641" }, { "type": "sorf", "sequence": "contig_2", "start": 16781, "stop": 16864, "strand": "-", "gene": null, "product": "PLD phosphodiesterase domain-containing protein", "frame": 1, "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A948C4F7", "UniRef:UniRef90_A0A948C4F7" ], "aa": "MILIDKKILLLGSMNLSANSLDNNREI", "aa_hexdigest": "e939df9deb0863b6f67955f2c0c9df4e", "nt": "ATGATCCTAATTGACAAGAAAATTCTTCTGCTTGGAAGTATGAATCTCTCTGCGAATAGTTTAGATAATAATAGAGAAATCTGA", "psc": { "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.963, "score": 48.9, "evalue": 6.92e-12, "uniref90_id": "UniRef90_A0A948C4F7", "product": "PLD phosphodiesterase domain-containing protein", "uniref50_id": "UniRef50_A0A948C4F7" }, "pscc": { "uniref50_id": "UniRef50_A0A948C4F7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A948C4F7" ], "product": "PLD phosphodiesterase domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_2136", "locus": "GCA003260355_01642" }, { "type": "cds", "sequence": "contig_2", "start": 17054, "stop": 17560, "strand": "-", "frame": 1, "gene": null, "product": "phospholipase D", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4KCR0", "UniRef:UniRef90_A0A328EGM9" ], "nt": "ATGCCGAATCCTGACTATCTCAAAAACTACGAAAGCTCCCTTCAAAACAGCCCAAGGATCCAGCTTCAAACCTACGAATTTACCGAGAAACATCTTAAATTAAAACTCAAAAAGCTGGCAGAAAACGGAGTGAAGATCCAGATTATGATTGAAAACAAGAAATATCAGCAATTCAAAAATACGTATAAAGAACTCCAAAATTACTTTTCAGGACTGCAAAATATTGAAGTGAAATCTGATGAGCATCTGCCGACTCAATATCTTCATAGCAAGATTACCCTGATGCAGAGCGGATTTTGGATTCAGAGTTCTAATCTTACCCATTCCACTTTCGCAAAGAACCGTGAACACCTTTTCCGAAGTGAAAACCAAGAAGTGCTAAAAAGTCTGCATACGCTGTTTGAAAAGGACCGAAAAGGTGAACAACTTCTCCCTTCCGACCTGCATCCTAATCTTGTCGTTTGCAATCTCAATTGCCGTGCGGTGATTACGGAGCTGATTTCCTGA", "aa": "MPNPDYLKNYESSLQNSPRIQLQTYEFTEKHLKLKLKKLAENGVKIQIMIENKKYQQFKNTYKELQNYFSGLQNIEVKSDEHLPTQYLHSKITLMQSGFWIQSSNLTHSTFAKNREHLFRSENQEVLKSLHTLFEKDRKGEQLLPSDLHPNLVVCNLNCRAVITELIS", "aa_hexdigest": "304dced002882f50b02526636526d4b7", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 16751, "stop": 17728, "observations": { "mutation": [ 16783, 16912, 17056, 17638 ], "stop": [ 16783, 16912, 17056, 17638 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EGM9", "query_cov": 1.0, "subject_cov": 0.4772727272727273, "identity": 0.9640000000000001, "score": 318.0, "evalue": 3.46e-107, "gene_start": 1, "gene_end": 168, "reference_start": 57, "reference_end": 224, "reference_sequence": "MKKLLIALIAALLALLLSPGVEFRFGGEEKGAEKSDFSLSSDWLEHIENITGMIVQMPNPDYLKNYESSLENSPMIQLQTYEFTEKHLKSKLKKLAENGVKIQIMIENKKYQQFKNTYKELQNYFSGLQNIEVKSDEHLPTQYLHSKITLMQSGFWIQSSNLTHSTFAKNREHLFRSEDPKVLKSLHTLFEKDRKGEQLLPSDLHPNLVVCNLNCRAVITELISGAKESIIMQTQYLNDPALFDLIAKKSEILNFKAIFSDTETNQSLPDYFGTHQVRLMKKPYVHTKMILIDKKILLLGSMNLSANSLDNNREIGILLIDPKLIEQFLSEFRADWEAIKHPSPTSKRAKKI" }, "paralog": false, "identity": 0.9538461538461539, "score": 608.0, "evalue": 6.39e-224, "description": "Internal stop codon at 16783, 16912, 17056, 17638. Nonsense mutation around 16783, 16912, 17056, 17638.", "psc": { "uniref90_id": "UniRef90_A0A328EGM9", "product": "phospholipase D", "uniref50_id": "UniRef50_A0A2W4KCR0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EGM9", "UniRef:UniRef50_A0A2W4KCR0" ] }, "pscc": { "uniref50_id": "UniRef50_A0A2W4KCR0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4KCR0" ], "product": "phospholipase D" } }, "id": "NKOCPLAKPK_1661", "locus": "GCA003260355_01643" }, { "type": "cds", "sequence": "contig_2", "start": 18111, "stop": 18278, "strand": "-", "frame": 3, "gene": null, "product": "GIY-YIG domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1L9GSK7", "UniRef:UniRef90_A0A328ECN7" ], "nt": "ATGAAAAGAGCCTATGTCTATATTCTGAGTAATAAAAGGAATGGGACTCTTTATGTAGGAGTAACCAGTAATCTTGTGAAAAGAATCTTTGAACACAAAGAAAAGGTTGTAAATGGTTTTTCTGCTAAGTATAATCTTGATTTACTCGTATATTATGAGGAACGGTGA", "aa": "MKRAYVYILSNKRNGTLYVGVTSNLVKRIFEHKEKVVNGFSAKYNLDLLVYYEER", "aa_hexdigest": "70af13a10fbece9db43cf8f08e55a491", "start_type": "ATG", "rbs_motif": "TTATT", "psc": { "uniref90_id": "UniRef90_UPI00345DA75D", "query_cov": 0.9818181818181818, "subject_cov": 0.8059701492537313, "identity": 0.63, "score": 75.9, "evalue": 4.36e-17, "valid": false, "product": "GIY-YIG nuclease family protein", "uniref50_id": "UniRef50_A0A833FWK1" }, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 17991, "stop": 18278, "observations": { "mutation": [ 18113 ], "stop": [ 18113 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328ECN7", "query_cov": 1.0, "subject_cov": 0.5789473684210527, "identity": 0.982, "score": 109.0, "evalue": 3.57e-30, "gene_start": 1, "gene_end": 55, "reference_start": 1, "reference_end": 55, "reference_sequence": "MKGAYVYILSNKRNGTLYVGVTSNLVKRIFEHKEKVVNGFSAKYNLDLLVYYEERGSIEEAIQREKQLKAGNRKKKLGLIESINPDWKDLSEYLE" }, "paralog": false, "identity": 0.968421052631579, "score": 182.0, "evalue": 1.84e-61, "description": "Internal stop codon at 18113. Nonsense mutation around 18113.", "psc": { "uniref90_id": "UniRef90_A0A328ECN7", "product": "GIY-YIG domain-containing protein", "uniref50_id": "UniRef50_A0A1L9GSK7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328ECN7", "UniRef:UniRef50_A0A1L9GSK7" ] }, "pscc": { "uniref50_id": "UniRef50_A0A1L9GSK7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1L9GSK7" ], "product": "GIY-YIG domain-containing protein" } }, "id": "NKOCPLAKPK_1662", "locus": "GCA003260355_01644" }, { "type": "cds", "sequence": "contig_2", "start": 18489, "stop": 18752, "strand": "-", "frame": 3, "gene": null, "product": "Dephospho-CoA kinase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MW99", "UniRef:UniRef90_A0A328EG09" ], "nt": "GTGGAAGCACTAAAAAAGAAGTCTGATTTCCTTCTTCTTTCAGTAGATGCGGATCAGAGACTCAGATACGAAAGAGCGATAAAAAGGAACTCTGTGAAAGATCAGATTACTTGGGAACATTTTCAGGAGCAAGAGGCTTTGGAAGCGAATAATACTGACCCTAATAAAGGGAATATTCTCGCCTGCCAGAAGCTAGCAGATATTCAGCTTGATAATAATGGCAGTTTGGAAGAACTGTATGAGCAGATTGATCGTGTAGTATGA", "aa": "MEALKKKSDFLLLSVDADQRLRYERAIKRNSVKDQITWEHFQEQEALEANNTDPNKGNILACQKLADIQLDNNGSLEELYEQIDRVV", "aa_hexdigest": "8c4ebbc4e769676f5d2cff4e599143eb", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 18477, "stop": 19031, "observations": { "mutation": [ 18491, 18842, 18995, 19010 ], "stop": [ 18491, 18842, 18995, 19010 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EG09", "query_cov": 1.0, "subject_cov": 0.47282608695652173, "identity": 0.966, "score": 165.0, "evalue": 2.41e-50, "gene_start": 1, "gene_end": 87, "reference_start": 94, "reference_end": 180, "reference_sequence": "MKIIGITGPQAAGKGAVVEYLVKKLGFVHYSVSGYLTEQLQAQGQEINRDTMRVLADSLRAKFGPAYSIEQLYSQAEKNGQDAIIESIRTIGEVEALKKKSDFLLLSVDADQRLRYERAIKRNSVKDQITWEHFQEQEALEANNTDPNKGNILACQKLADIQFDNNGSLEELYEQIDRIVGMNG" }, "paralog": false, "identity": 0.9565217391304348, "score": 341.0, "evalue": 2.91e-122, "description": "Internal stop codon at 18491, 18842, 18995, 19010. Nonsense mutation around 18491, 18842, 18995, 19010.", "psc": { "uniref90_id": "UniRef90_A0A328EG09", "product": "Dephospho-CoA kinase", "uniref50_id": "UniRef50_A0A660MW99", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EG09", "UniRef:UniRef50_A0A660MW99" ] }, "pscc": { "uniref50_id": "UniRef50_A0A660MW99", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MW99" ], "product": "Dephospho-CoA kinase" } }, "id": "NKOCPLAKPK_1663", "locus": "GCA003260355_01645" }, { "type": "cds", "sequence": "contig_2", "start": 18840, "stop": 18950, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCATTATTCGGTTAGTGGCTATCTCACGGAGCAGTTACAAGCGCAAGGGCAAGAAATTAACAGAGACACAATGAGAGTGCTCGCTGATAGCTTAAGAGCGAAATTTTGA", "aa": "MHYSVSGYLTEQLQAQGQEINRDTMRVLADSLRAKF", "aa_hexdigest": "198e6f6b261942db70dbbbfea0d3936c", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4157.6444, "isoelectric_point": 6.518659400939941 }, "id": "NKOCPLAKPK_1664", "locus": "GCA003260355_01646" }, { "type": "cds", "sequence": "contig_2", "start": 19064, "stop": 19267, "strand": "-", "frame": 1, "gene": null, "product": "DNA-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4IKN1", "UniRef:UniRef90_A0A328EBY7" ], "nt": "ATGGAAGAGATCCCCAAGCGGATTGCTCACGGATATTCGGTGCAGCTTGGTGAGATCGGGACGCTCCGCCTGAGTCTTTCAAGTGAAGGAGTGGATGATACCAAGCATTTTAATCCAAGGACCATGAAGAAAAAGGTTGTTTTCCTGCCTTCAAAAGCTTTTAAATCAGAGTTAAAAAACATTTCCTTTGAATACAAAAAGTAA", "aa": "MEEIPKRIAHGYSVQLGEIGTLRLSLSSEGVDDTKHFNPRTMKKKVVFLPSKAFKSELKNISFEYKK", "aa_hexdigest": "f9882cf88f68af37b25c5915de383d65", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 19064, "stop": 19435, "observations": { "mutation": [ 19297 ], "stop": [ 19297 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EBY7", "query_cov": 1.0, "subject_cov": 0.5447154471544715, "identity": 0.985, "score": 130.0, "evalue": 5.65e-38, "gene_start": 1, "gene_end": 67, "reference_start": 57, "reference_end": 123, "reference_sequence": "MHYRFVAKANPLDQSRKKWYATSVNTGKIATRQIAKTLADKSSLTPGDVLNVLENLMEEIPKRIAHGYSVQLGEIGTLRLSLSSEGVDDTKHFNPRTIKKKVVFLPSKAFKSELKNISFEYKK" }, "paralog": false, "identity": 0.983739837398374, "score": 239.0, "evalue": 2.4e-83, "description": "Internal stop codon at 19297. Nonsense mutation around 19297.", "psc": { "uniref90_id": "UniRef90_A0A328EBY7", "product": "DNA-binding protein", "uniref50_id": "UniRef50_A0A2W4IKN1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EBY7", "UniRef:UniRef50_A0A2W4IKN1" ] }, "pscc": { "uniref50_id": "UniRef50_A0A2W4IKN1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4IKN1" ], "product": "Viral histone-like protein" } }, "id": "NKOCPLAKPK_1665", "locus": "GCA003260355_01647" }, { "type": "cds", "sequence": "contig_2", "start": 19295, "stop": 19465, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTGATTTTATTCCTTTATTCTCTTAAAATGCACTATCGTTTCGTCGCAAAGGCAAATCCTCTAGATCAGAGCCGTAAGAAGTGGTATGCGACTTCGGTCAATACGGGTAAGATTGCTACTAGGCAGATTGCAAAGACTCTTGCTGATAAGTCTTCACTGACACCTTGA", "aa": "MLILFLYSLKMHYRFVAKANPLDQSRKKWYATSVNTGKIATRQIAKTLADKSSLTP", "aa_hexdigest": "9fff0fbdd0181caccb9cb7244246adb5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6416.518400000003, "isoelectric_point": 10.354412269592284 }, "id": "NKOCPLAKPK_1666", "locus": "GCA003260355_01648" }, { "type": "cds", "sequence": "contig_2", "start": 19626, "stop": 19748, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTTTTTATATCTCTCTTGAAAGGACTTTGATTCTTACCTGTGAGATGTTTTTTTATGGAGGCAAACAAAAGGATTCTTTATTAGTCAGGAGAAGTATTGGTTATTCAATTTATAGTTGA", "aa": "MIFYISLERTLILTCEMFFYGGKQKDSLLVRRSIGYSIYS", "aa_hexdigest": "13db2e7695ba282a14bc635556e8e1d6", "start_type": "ATG", "rbs_motif": "TTTA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4743.5659000000005, "isoelectric_point": 8.977689933776855 }, "id": "NKOCPLAKPK_1667", "locus": "GCA003260355_01649" }, { "type": "cds", "sequence": "contig_2", "start": 19843, "stop": 20109, "strand": "-", "frame": 2, "gene": null, "product": "Coenzyme F420:L-glutamate ligase-like domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EEI5", "UniRef:UniRef90_A0A328EEI5" ], "nt": "TTGTATAGTTATGGATTTTATCCTCTAATTGATAAAAGAGGAGAACAAGATCTTTTTGGAAAAACTATGGAACTTACTCAGATTAATCTTCCAGAATCCCTCAGTAGCTTTGTAGTCTTTCTTATGGGAGAAACTAATGAACAAACCCCAATGCTTATTGCAAGAGGAATGCCTCATATTCATTATACTGTAGAGGATCTTTATCCTCAAACGATTGTTCCTATTGATCAAGATATTTATGCTCCTTTATTAAAAAATTTCCTTTAA", "aa": "MYSYGFYPLIDKRGEQDLFGKTMELTQINLPESLSSFVVFLMGETNEQTPMLIARGMPHIHYTVEDLYPQTIVPIDQDIYAPLLKNFL", "aa_hexdigest": "011b0124bcb6792a89046e82155b15e4", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 19843, "stop": 20409, "observations": { "mutation": [ 20169, 20331 ], "stop": [ 20169, 20331 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EEI5", "query_cov": 1.0, "subject_cov": 0.43137254901960786, "identity": 0.9890000000000001, "score": 183.0, "evalue": 3.34e-57, "gene_start": 1, "gene_end": 88, "reference_start": 117, "reference_end": 204, "reference_sequence": "MLFISSKILAVHQGRCVKIGTAEKKSLIEKESEKYLINEHIPGGLYLTITDNTLIPSAGIDESNANGYYILLPKDYQEEVKKLHTCLLKKYQINQLGIIITDTTSRPLRYGTAGIALYSYGFYPLIDKRGEQDLFGKTMELTQINLPESLSSFVVFLMGETNEQTPMLIARGMPHIHYTVEDLYPQTIVPIDQDIYAPLLKNFL" }, "paralog": false, "identity": 0.9893617021276596, "score": 371.0, "evalue": 8.23e-134, "description": "Internal stop codon at 20169, 20331. Nonsense mutation around 20169, 20331.", "psc": { "uniref90_id": "UniRef90_A0A328EEI5", "product": "Coenzyme F420:L-glutamate ligase-like domain-containing protein", "uniref50_id": "UniRef50_A0A328EEI5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EEI5", "UniRef:UniRef50_A0A328EEI5" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EEI5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EEI5" ], "product": "Coenzyme F420:L-glutamate ligase-like domain-containing protein" } }, "id": "NKOCPLAKPK_1668", "locus": "GCA003260355_01650" }, { "type": "cds", "sequence": "contig_2", "start": 20744, "stop": 21040, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATTTATTTGGATGGTGAAATCTTAGCAGCTTATGAGAGAATCCCTCCAAAGGTAGTTGGGAATGGCATAAGTTCTATAGGTCAGCTTATTGATAAAGATTTTCAAGATCTTGAAATAAATAAAATTACTACCTATCTGCAACAGCAGAATCTGTCTTTAGATTCTGTGTTGCCTCAGGATTTTGAATTTAATCTCTTGCCTACAGCAAATATTGCTACAGGAGGTTTTGTGCGTGAGGTACAAGCGACTGAGACAGATAGGCTCTTTTTAGAAAGGGTGGCAAAATATTTCTGA", "aa": "MIYLDGEILAAYERIPPKVVGNGISSIGQLIDKDFQDLEINKITTYLQQQNLSLDSVLPQDFEFNLLPTANIATGGFVREVQATETDRLFLERVAKYF", "aa_hexdigest": "8469f162bbd8cb47b188d070962477f3", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11082.47029999999, "isoelectric_point": 4.397883033752441 }, "id": "NKOCPLAKPK_1669", "locus": "GCA003260355_01651" }, { "type": "cds", "sequence": "contig_2", "start": 21053, "stop": 21280, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTATCATCAAAGATAGATCATCTTATAGTACAACTTCAAATAATACTTTAGCATGTTTGGATTTTGCTAAAAAAAACGGATATCCTTTGATTTTTAAACCAAATGATGGCAGTTTGGGGGTTGGAGTGCAAAAAATTTTCAATCAAGAACAATTACTAAAGGCCCTTGAAGACTATAATGCAAGCAATAAAGGACTCTATCTCTTACAACAATATATCCCATGA", "aa": "MIIIKDRSSYSTTSNNTLACLDFAKKNGYPLIFKPNDGSLGVGVQKIFNQEQLLKALEDYNASNKGLYLLQQYIP", "aa_hexdigest": "5690c518f6fa4b34145fa62dd523c91d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8416.547999999999, "isoelectric_point": 8.759206581115723 }, "id": "NKOCPLAKPK_1670", "locus": "GCA003260355_01652" }, { "type": "cds", "sequence": "contig_2", "start": 21302, "stop": 21427, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACGATTCAGCTTCCAAGCGGTAAAAATAGAATTATTTATAATGCTGACTATGGATTTGATAGTTCATCATTAAGAAGGATTTTTGAAGATAAGATATATACCTCTAAGATTCTTAGGAACTGA", "aa": "MTIQLPSGKNRIIYNADYGFDSSSLRRIFEDKIYTSKILRN", "aa_hexdigest": "796e93eef4d1b6a33b5516850c7f2670", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4825.4598, "isoelectric_point": 9.693224525451662 }, "id": "NKOCPLAKPK_1671", "locus": "GCA003260355_01653" }, { "type": "cds", "sequence": "contig_2", "start": 22031, "stop": 22249, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTGTCAAACCGAATAAAGGAGAAAGAGGGAATGGGGTTATGCTTCTTAGTAATCTCAAAGAGTTAGAACAATTCTATCAAGAATATAGTAATGGTCTCTTTGATGAGAAGATAGCCGTTATTCAGGAAAAAGTTAAGGGGAATGAATATAGGGTTATTTACTTAGATGGAGAGATTCTGCTCACATATATGAAACAGCCATTTACCTTAGTTTGA", "aa": "MIVKPNKGERGNGVMLLSNLKELEQFYQEYSNGLFDEKIAVIQEKVKGNEYRVIYLDGEILLTYMKQPFTLV", "aa_hexdigest": "b40f46758b35fd6bd4ae5b3944ab9fe4", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8387.6557, "isoelectric_point": 5.342262840270995 }, "id": "NKOCPLAKPK_1672", "locus": "GCA003260355_01654" }, { "type": "cds", "sequence": "contig_2", "start": 22860, "stop": 23597, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328ECQ2", "UniRef:UniRef90_A0A328ECQ2" ], "nt": "ATGAAGAATAGCCTTGATTTCTCTTTTAGTTTTATCATACTGGCAATCGCAATGAAGTGGTCATTAACATCAGTATTAATTAAAACGTATAAAGAAATGGAAAGGATAGTTAAAAAAAAGAGCTGGAGAACGCTTGTTTTCGCTCTAGGTATAGTGTTTCTGATTGGAGGCGGTATCTTTATCGCTTGTCAAAAGGAAGCTAATGTGGAAGTTTTGGATTCTCAGCAAGATCATGGGGTAGAATATCCTATTCCTGAAGGTGCGAAGGCATTAATTATGGATGTTCATTTTTACAAAGATTTATATCCAGATTATGAAGATCCTCTAGGGGAGAAAGGCGATATGCTTATTCTTGATCCAGCAGCAGCATCCTATTATCTTCCTCATGATATTAAGCATTATGAGGAGTATGCTCGTTTGATAAAAGGAGCAGGGGAGGTTTATCCTCGTAAGTTTAATCTCCTTAAAATTACGGTGGGGCACGATACTCGTGGGGTTGGAGCCATCCCTATCATTAAGGTAGAGCGTCCAACTCCGGAGGAATATGAGAAACACTTGGACTGGTATATAAAGGCTAATTCTGGAGCGGAGAGCACAGTGAGTACACGATCCGCTCGTTCTTTAGCGGTATCTATGCCACTCTCTAGAGTGCAACAATTTTTCAGTCAATTTGAACGAATTAGCTGCGCGAACAATCCCAATCAATGTCCTTGTATTTCCTTTCAATATTGCTGTTGA", "aa": "MKNSLDFSFSFIILAIAMKWSLTSVLIKTYKEMERIVKKKSWRTLVFALGIVFLIGGGIFIACQKEANVEVLDSQQDHGVEYPIPEGAKALIMDVHFYKDLYPDYEDPLGEKGDMLILDPAAASYYLPHDIKHYEEYARLIKGAGEVYPRKFNLLKITVGHDTRGVGAIPIIKVERPTPEEYEKHLDWYIKANSGAESTVSTRSARSLAVSMPLSRVQQFFSQFERISCANNPNQCPCISFQYCC", "aa_hexdigest": "0fcc3efeed1449c7ee76b6589a827bae", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A328ECQ2", "query_cov": 0.9306122448979591, "subject_cov": 0.9956331877729258, "identity": 1.0, "score": 466.0, "evalue": 3.71e-166, "valid": true, "uniref50_id": "UniRef50_A0A328ECQ2" }, "pscc": { "uniref50_id": "UniRef50_A0A328ECQ2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328ECQ2" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 27804.815600000024, "isoelectric_point": 6.598063468933106 }, "id": "NKOCPLAKPK_1673", "locus": "GCA003260355_01655" }, { "type": "cds", "sequence": "contig_2", "start": 23587, "stop": 24162, "strand": "-", "frame": 2, "gene": null, "product": "RNA polymerase sigma-70 region 2 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBF637C", "UniRef:UniRef100_A0A328EFU1", "UniRef:UniRef50_A0A328EFU1", "UniRef:UniRef90_A0A328EFU1" ], "nt": "ATGACTGTTATTAAGTTTGAACAAGCATTTCTTCTTCTTCTAATCCAACAGGATGCTGGAGCCTACAATACTTTTTACCTCCAGACGGTTGATATTTTTTTCCGTTATCTCCAGAGCAACTTCTTTCTCTCTCAAGAGGATTCAGAAGATATTATCGCAGATTTCTATGTAAAATTCCGAAACGCAGTGACGAAATACGATATCAATCAGTCTTTTTCTGCTTATGTATGGGTGATTTTCAAGAATACTGTCAAAGATGCCTTCAAAAAGAATAGAGAACTCCCCTTCTCTCTCCTAGATGGTGATGATGAAGAGGGAGAAAAGTTTGAAGATACGCTTGTGGACGAGGGCGTAATTGATGAGTTTTTACAGCAAGACTTTGAACTCGAGCAGATCAAGAAAGCGATGCAGGAACTTGACGCTTTGAGTAGAGAAATTATTTTTCTCAAATATATAGAACACAAGTCAAATACTGAAATAGAGGAACAGTTGATGATTTCGCAGGAAGTGATCAGACAGAGAATTTCAAGAGCTTTCAAAAAACTGAGAGTGCTCCTAAAATGCGATGAAGAATAG", "aa": "MTVIKFEQAFLLLLIQQDAGAYNTFYLQTVDIFFRYLQSNFFLSQEDSEDIIADFYVKFRNAVTKYDINQSFSAYVWVIFKNTVKDAFKKNRELPFSLLDGDDEEGEKFEDTLVDEGVIDEFLQQDFELEQIKKAMQELDALSREIIFLKYIEHKSNTEIEEQLMISQEVIRQRISRAFKKLRVLLKCDEE", "aa_hexdigest": "6c78aa2ff3e147242df58086e88740b8", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000DBF637C", "uniref100_id": "UniRef100_A0A328EFU1", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBF637C", "UniRef:UniRef100_A0A328EFU1" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EFU1", "uniref90_id": "UniRef90_A0A328EFU1" }, "psc": { "uniref90_id": "UniRef90_A0A328EFU1", "product": "RNA polymerase sigma-70 region 2 domain-containing protein", "uniref50_id": "UniRef50_A0A328EFU1" }, "pscc": { "uniref50_id": "UniRef50_A0A328EFU1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EFU1" ], "product": "RNA polymerase sigma-70 region 2 domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_1674", "locus": "GCA003260355_01656" }, { "type": "cds", "sequence": "contig_2", "start": 24734, "stop": 24970, "strand": "-", "frame": 1, "gene": null, "product": "2Fe-2S ferredoxin-type domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MW63", "UniRef:UniRef90_A0A660MW63" ], "nt": "ATGCACGGAGTGGAAATCCTTCAGTCTTGTGGAACAGGATTTTGTGGGGTTTGCCTGTGCGATGTCGTAGAAGGAGGAGACGCAGTCGAGCTTAATAAGACGGGCGAACACGTTTTTGAGGTGGCAATGGACGAAGGAAATCATCCAAAGCAGCTTCTTGCCTGTGTGAGTGGGGTTAAGTCTGAATATTTTTCCGATGAAAAAGATCATACCATTGTGATCAAAAAACTCTACTAA", "aa": "MHGVEILQSCGTGFCGVCLCDVVEGGDAVELNKTGEHVFEVAMDEGNHPKQLLACVSGVKSEYFSDEKDHTIVIKKLY", "aa_hexdigest": "87efa826f8c914b8fff29a00418545e6", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 24734, "stop": 25111, "observations": { "mutation": [ 25018 ], "stop": [ 25018 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A660MW63", "query_cov": 1.0, "subject_cov": 0.624, "identity": 0.987, "score": 168.0, "evalue": 1.57e-52, "gene_start": 1, "gene_end": 78, "reference_start": 48, "reference_end": 125, "reference_sequence": "MMSILLHSKHFISFKAMTVIVKVQDPTGNEIGSFEAEDQKSLGELAAMHGVEILQSCGTGFCGVCLCDVVEGGEAVELNKTGEHVFEVAMDEGNHPKQLLACVSGVKSEYFSDEKDHTIVIKKLY" }, "paralog": false, "identity": 0.984, "score": 250.0, "evalue": 2.12e-87, "description": "Internal stop codon at 25018. Nonsense mutation around 25018.", "psc": { "uniref90_id": "UniRef90_A0A660MW63", "product": "2Fe-2S ferredoxin-type domain-containing protein", "uniref50_id": "UniRef50_A0A660MW63", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A660MW63", "UniRef:UniRef50_A0A660MW63" ] }, "pscc": { "uniref50_id": "UniRef50_A0A660MW63", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MW63" ], "product": "2Fe-2S ferredoxin-type domain-containing protein" } }, "id": "NKOCPLAKPK_1675", "locus": "GCA003260355_01657" }, { "type": "cds", "sequence": "contig_2", "start": 25204, "stop": 25293, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGACTTTTCTATGATTTTGGGAGAAGCAAAAGACACGATAGATCGCTTTAAGCAAGGACACCCTGATGGTGTCGTTATTATTCGATGA", "aa": "MDFSMILGEAKDTIDRFKQGHPDGVVIIR", "aa_hexdigest": "8141fec3fa11320225ebd6ab38c114d4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3289.7812000000004, "isoelectric_point": 5.451109504699706 }, "id": "NKOCPLAKPK_1676", "locus": "GCA003260355_01658" }, { "type": "cds", "sequence": "contig_2", "start": 25390, "stop": 25779, "strand": "+", "frame": 1, "gene": "miaA", "product": "tRNA (adenosine(37)-N6)-dimethylallyltransferase MiaA", "db_xrefs": [ "EC:2.5.1.75", "GO:0005737", "GO:0006400", "GO:0052381", "SO:0001217", "UniRef:UniRef50_A0A288DJJ6", "UniRef:UniRef90_A0A660MW98" ], "nt": "ATGGATATTGGGACGGATAAAATTAGTGCAGAAATCAGAGCTAAGATTCCTCATCATCAGATAGATATTGTAAATCCTGATGAACAATATACGGCTGGGCAGTGGAAACAAGCTGTAGATCAACAAATGGATACTATTCTCAAAAAAGGGAAAACACCTTTTATCGTCGGTGGGACTGGACTTTATATTGATTCAGTTTATAAGAACTTTTCTATGCCTGAATGTCCTCCAGATCCAGTATTGAGAAGAGAATTAGAAGAGAAGGAAATAGCAAATCCTGGCTTTCTTCATCGCGAACTTTCACGCATTGATCCTGAAGAAGCTCAAAAGCTTCATCCTCATTCGCTCAGGTATCTTATTAGAGCTTTGGAAATTTATTACAGTACTTGA", "aa": "MDIGTDKISAEIRAKIPHHQIDIVNPDEQYTAGQWKQAVDQQMDTILKKGKTPFIVGGTGLYIDSVYKNFSMPECPPDPVLRRELEEKEIANPGFLHRELSRIDPEEAQKLHPHSLRYLIRALEIYYST", "aa_hexdigest": "c197c153b6972d4b62aa02f94c62e143", "start_type": "ATG", "rbs_motif": null, "genes": [ "miaA" ], "truncated": "both", "pseudogene": { "start": 25204, "stop": 26082, "observations": { "mutation": [ 25291, 25777 ], "stop": [ 25291, 25777 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A660MW98", "query_cov": 1.0, "subject_cov": 0.4069400630914827, "identity": 1.0, "score": 261.0, "evalue": 9.42e-86, "gene_start": 1, "gene_end": 129, "reference_start": 63, "reference_end": 191, "reference_sequence": "MDFSMILGEAKDTIDRFKQGHPDGVVIIRGATATGKSRLSVELSKFFDIEVISSDSRQIFRKMDIGTDKISAEIRAKIPHHQIDIVNPDEQYTAGQWKQAVDQQMDTILKKGKTPFIVGGTGLYIDSVYKNFSMPECPPDPVLRRELEEKEIANPGFLHRELSRIDPEEAQKLHPHSLRYLIRALEIYYSTGTTKTESFREQAPRHPLLMIGLRRDKESTNQLINARIKEMFQNGLVNEVEMLLSEGYAPSLQSMQGIGYKEVIGYLLNEYTYEKIEELLKRNTHHLAKKQRTWFRRYLAEGIQSPKPGVSYKVFEL" }, "paralog": false, "identity": 0.9794520547945206, "score": 570.0, "evalue": 9.05e-210, "description": "Internal stop codon at 25291, 25777. Nonsense mutation around 25291, 25777.", "psc": { "uniref90_id": "UniRef90_A0A660MW98", "gene": "miaA", "product": "tRNA (adenosine(37)-N6)-dimethylallyltransferase MiaA", "ec_ids": [ "2.5.1.75" ], "uniref50_id": "UniRef50_A0A288DJJ6", "go_ids": [ "GO:0005737", "GO:0006400", "GO:0052381" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A660MW98", "EC:2.5.1.75", "UniRef:UniRef50_A0A288DJJ6", "GO:0005737", "GO:0006400", "GO:0052381" ] }, "pscc": { "uniref50_id": "UniRef50_A0A288DJJ6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A288DJJ6" ], "product": "tRNA dimethylallyltransferase" } }, "id": "NKOCPLAKPK_1677", "locus": "GCA003260355_01659" }, { "type": "cds", "sequence": "contig_2", "start": 25831, "stop": 26157, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01715.23" ], "nt": "ATGATTGGACTACGAAGAGATAAGGAATCCACCAATCAACTCATCAACGCAAGAATCAAAGAAATGTTTCAAAATGGGCTTGTCAATGAGGTCGAAATGCTTTTAAGCGAAGGTTATGCTCCGAGTCTTCAATCTATGCAGGGGATTGGCTACAAAGAAGTAGTTGGCTATCTTTTGAACGAATATACCTATGAAAAAATGGAAGAATTACTCAAAAGAAATACACACCACCTTGCCAAGAAGCAAAGGACTTGGTTTAGAAGATATCTTGCAGAGGGGATTCAAGCTCCAAAACCAGGCGTAAGCTACAAAGTTTTTGAACTCTAA", "aa": "MIGLRRDKESTNQLINARIKEMFQNGLVNEVEMLLSEGYAPSLQSMQGIGYKEVVGYLLNEYTYEKMEELLKRNTHHLAKKQRTWFRRYLAEGIQAPKPGVSYKVFEL", "aa_hexdigest": "25e93a053a1aa1c91d90d7310feaa5b8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01715.23", "name": "IPPT", "length": 88, "aa_cov": 0.8148148148148148, "hmm_cov": 1.0, "evalue": 1.2220153106446461e-28, "score": 96.83207702636719, "start": 1, "stop": 88 } ], "seq_stats": { "molecular_weight": 12657.48479999999, "isoelectric_point": 9.154526710510254 }, "id": "NKOCPLAKPK_1678", "locus": "GCA003260355_01660" }, { "type": "cds", "sequence": "contig_2", "start": 26311, "stop": 26532, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCATCTCTGTCTTTGGGGTGGACTTGGACTAGCAGTTATCGGTATTTATACCTATATTCTTCAGTGGATCCCATTTATTCAATCTCTAGTAGAGTCCGTTGGTTCTTTGCCGGTGGTAGTTACTGGTTCAGGAGTGATTATTATCGTTGGTGTAGTGCAGGAGATTATGAATAAGATCCAGGGAGATCTTGTAATGCAAAAATATGAAGCTTACGAATAG", "aa": "MHLCLWGGLGLAVIGIYTYILQWIPFIQSLVESVGSLPVVVTGSGVIIIVGVVQEIMNKIQGDLVMQKYEAYE", "aa_hexdigest": "481d4e7b9734d1022e34ec8d580d10f8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7994.451200000003, "isoelectric_point": 4.730788993835449 }, "id": "NKOCPLAKPK_1679", "locus": "GCA003260355_01661" }, { "type": "cds", "sequence": "contig_2", "start": 26593, "stop": 26976, "strand": "-", "frame": 2, "gene": "secY", "product": "Preprotein translocase subunit SecY", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A351Y4P0" ], "nt": "GTGCTTGTTCTTGTAGTGCTTTCTATCTTGATTCTCAAGTCAATTAAGGAAATTCCAATTATCTACGCAAAGCAGGGTAAGGCTCAGCAGTCATCTGCGCTTCCTATCCCTCTCAATCCTGTGGGAATGGTGCCTATCATCTTTGCAATGGCTTTCGTTTCTTTTCCTCAGCTGATAGCAAAGTTGATTACTCAATTTCAGCCAGCAAACTTAAAGCTTGTAGCAGTAGCCAATCGAATAGAGTCGCATTTCAATATCTATGCTCAAAATCCAGGAGTATTGGCGATTGCTGTATATGTGCTTTTCATCATCCTGTTCACTTTCTTTTATGCGATGATTACCTTTAGTCCTGATAGGATTTCAGATAACATTCAGAAAAGATGA", "aa": "MLVLVVLSILILKSIKEIPIIYAKQGKAQQSSALPIPLNPVGMVPIIFAMAFVSFPQLIAKLITQFQPANLKLVAVANRIESHFNIYAQNPGVLAIAVYVLFIILFTFFYAMITFSPDRISDNIQKR", "aa_hexdigest": "cd6028974d8cdd763dc927848459bf49", "start_type": "GTG", "rbs_motif": "TTTTTA", "psc": { "uniref90_id": "UniRef90_A0A351Y4P0", "query_cov": 0.968503937007874, "subject_cov": 0.8661971830985915, "identity": 0.7070000000000001, "score": 171.0, "evalue": 8.51e-53, "valid": false, "product": "Preprotein translocase subunit SecY", "uniref50_id": "UniRef50_A0A351Y4P0" }, "pscc": { "uniref50_id": "UniRef50_A0A351Y4P0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A351Y4P0" ], "product": "Preprotein translocase subunit SecY" }, "genes": [ "secY" ], "id": "NKOCPLAKPK_1680", "locus": "GCA003260355_01662" }, { "type": "cds", "sequence": "contig_2", "start": 27001, "stop": 27444, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTCCTTGGAGGTGCTTTAGATAAGTTTGCCTTTATTGCGATTGGAATCTCTCCATATATTACGGCATCTATTATTATGCAGCTTCTTGGGGTGATTGTCCCTGCTGTTGAAGAGCTTTCAGAGCAAGGAGAAGTGGGCCAGGCAAAGATCAACCAATATACAAGATATCTAACCTTACCATTGGCTTTTTTACAGGGTATCGGATCCGTATATCTTGTGAATAATATGCTTGGAGGAGCAGTAATCTCAACAGATATTACAACAGTGCTTTTGTCTGGATTTGCTATGGCGGTTGGTTCAATTCTTCTTATGCGGATTGGAGAGTTAATTACAGAAAAAGGAATCTCAAATGGAATTTCAATGCTTATCTTTGCTTCTATCGTGGCAGGAATTACTCAGCAGATTTCAAGAGATTTCTCCTCATCTAGTAATATCTGGTGA", "aa": "MLLGGALDKFAFIAIGISPYITASIIMQLLGVIVPAVEELSEQGEVGQAKINQYTRYLTLPLAFLQGIGSVYLVNNMLGGAVISTDITTVLLSGFAMAVGSILLMRIGELITEKGISNGISMLIFASIVAGITQQISRDFSSSSNIW", "aa_hexdigest": "ed1e44d2a9a5b16b3cfb0f33fed5cee1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15544.12269999999, "isoelectric_point": 4.648372459411622 }, "id": "NKOCPLAKPK_1681", "locus": "GCA003260355_01663" }, { "type": "cds", "sequence": "contig_2", "start": 27454, "stop": 27639, "strand": "-", "frame": 2, "gene": "secY", "product": "Preprotein translocase subunit SecY", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3D0UTM8" ], "nt": "ATGAAAGCATTAAAAACCTTTAATAAGAAAGTTCGTGAAGTTTTTGGAAACAGAAAAATCAGAAATAAAATTCTGTTTACCATTGGAATGTTGGCGATATACAGACTATTAGTCTTTATTCCTATTCCATTTGCTGATATCACCGCTTTGATGAGCCAAACTCTCAATGCGGGTTCTGAATTTTGA", "aa": "MKALKTFNKKVREVFGNRKIRNKILFTIGMLAIYRLLVFIPIPFADITALMSQTLNAGSEF", "aa_hexdigest": "c0d52a21441791133e5944428e74a732", "start_type": "ATG", "rbs_motif": "TTATT", "psc": { "uniref90_id": "UniRef90_A0A3D5YH76", "query_cov": 0.9016393442622951, "subject_cov": 0.9322033898305084, "identity": 0.6, "score": 73.2, "evalue": 5.36e-16, "valid": false, "product": "Preprotein translocase subunit SecY", "uniref50_id": "UniRef50_A0A3D0UTM8" }, "pscc": { "uniref50_id": "UniRef50_A0A3D0UTM8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3D0UTM8" ], "product": "Preprotein translocase subunit SecY" }, "genes": [ "secY" ], "id": "NKOCPLAKPK_1682", "locus": "GCA003260355_01664" }, { "type": "cds", "sequence": "contig_2", "start": 27771, "stop": 28085, "strand": "-", "frame": 3, "gene": "rplO", "product": "50S ribosomal protein L15", "db_xrefs": [ "GO:0003735", "GO:0006412", "GO:0015934", "KEGG:K02876", "SO:0001217", "UniRef:UniRef50_A0A7W4E3S1", "UniRef:UniRef90_A0A328ECP4" ], "nt": "ATGAAACCATTCTTTGAAGGAGGTCAGACATCTATTGTTCAGAGAATGCCTAAGCATAGAGGATTCAAGAGATATTACAAGCTTATTACTCCAATTCAAAATGTAAATCTTGGCTCTCTTCAGAAGGATGAAAGAATTACAGCAGTTGTGACTAAAGAACTCCTTTGGGAGCTTGGATACATTCGTTCTGCTGAGGTTGCAGTAAAGGTGCTTGGAACTGGAGATCTTGAAAAAGCTTTGTCTTTTGAAGGAATTGAACAGTATTCAGCTACTGCTAAGTCAAAGATTGAAAAAGCAGGAGGAAGCATAAAATAA", "aa": "MKPFFEGGQTSIVQRMPKHRGFKRYYKLITPIQNVNLGSLQKDERITAVVTKELLWELGYIRSAEVAVKVLGTGDLEKALSFEGIEQYSATAKSKIEKAGGSIK", "aa_hexdigest": "11d9f20ece5a1bb3751d29e8af6ca1f8", "start_type": "ATG", "rbs_motif": null, "genes": [ "rplO" ], "truncated": "3-prime", "pseudogene": { "start": 27771, "stop": 28220, "observations": { "mutation": [ 28190 ], "stop": [ 28190 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328ECP4", "query_cov": 1.0, "subject_cov": 0.697986577181208, "identity": 0.99, "score": 204.0, "evalue": 6.72e-66, "gene_start": 1, "gene_end": 104, "reference_start": 46, "reference_end": 149, "reference_sequence": "MKLHSLVRSKGLTDKLRRRGRGNASGRGNTSGRGANGQKSRSGHSMKPFFEGGQTSIVQRMPKHRGFKRYYKLITPIQNVNLGSLQKDERITAVVTKELLWELGYLRSAEVAVKVLGTGDLEKALSFEGIEQYSATAKSKIEKAGGSIK" }, "paralog": false, "identity": 0.9865771812080537, "score": 263.0, "evalue": 1.65e-91, "description": "Internal stop codon at 28190. Nonsense mutation around 28190.", "psc": { "uniref90_id": "UniRef90_A0A328ECP4", "gene": "rplO", "product": "50S ribosomal protein L15", "uniref50_id": "UniRef50_A0A7W4E3S1", "kegg_orthology_id": "K02876", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015934" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328ECP4", "UniRef:UniRef50_A0A7W4E3S1", "KEGG:K02876", "GO:0003735", "GO:0006412", "GO:0015934" ] }, "pscc": { "uniref50_id": "UniRef50_A0A7W4E3S1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7W4E3S1" ], "product": "Large ribosomal subunit protein uL15" } }, "id": "NKOCPLAKPK_1683", "locus": "GCA003260355_01665" }, { "type": "cds", "sequence": "contig_2", "start": 28252, "stop": 28701, "strand": "-", "frame": 2, "gene": null, "product": "Small ribosomal subunit protein uS5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7W4E3Q4", "UniRef:UniRef90_A0A328EG21" ], "nt": "GTGCCTTTGACAAAAAATGATACCGTTCCTTATCCAATCGCTTTGAAATATAAGGCATGTGTAGTTAAGCTTTTGCCAGCATCAGAGGGTACTGGACTTAAGGCAGGTTCATCTATCAGATCTGTTTTGGAACTTGCTGGATATTCAAATATCCTTTCTAAGATGGTTGGGTCAAACAACAAGCTTAATAATGCTTTAGCTACTATTAGAGCCTTATCAGCATATAAGCATGCGGATTACTTTTCTTCCCTTAAAGTTGCAAAAGTAGAGGAAGAATCTTCTACAAAAGAAGAAAAATCTGAGCCAACTGTTGAGTCTGCTCCTAAAGCAACTGCCTCAAAAAAAGAAGAGGTTGCTGAAGCTGGAGAAGCTCCAGCAAAGAAGGCAGCAGTAAAAAAGGCTCCAGCAAAGAAGGCTGCTCCAAAAAAGACAGAGAAAAAAGAAGACTAA", "aa": "MPLTKNDTVPYPIALKYKACVVKLLPASEGTGLKAGSSIRSVLELAGYSNILSKMVGSNNKLNNALATIRALSAYKHADYFSSLKVAKVEEESSTKEEKSEPTVESAPKATASKKEEVAEAGEAPAKKAAVKKAPAKKAAPKKTEKKED", "aa_hexdigest": "d65e9ce00371cc6f682eaec98f8eb4b8", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 28252, "stop": 28956, "observations": { "mutation": [ 28782 ], "stop": [ 28782 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EG21", "query_cov": 1.0, "subject_cov": 0.6367521367521367, "identity": 0.973, "score": 236.0, "evalue": 1.58e-76, "gene_start": 1, "gene_end": 149, "reference_start": 86, "reference_end": 234, "reference_sequence": "MMSMRSEKDNKRDVRREKPKKEFEELLLEVRRVTRVTTGGRRLSFRATILVGNKKGKIGLGISKGNDVASAVQKASHEAYKNLFEVPLTKNDTVPYPIALKYKACVVKLLPASEGTGLKAGSSIRSVLELAGYSNILSKMVGSNNKLNNALATIRALSAYKHADYFSSLKVAKVEEESSTKEEKSEPTVESAPKATASKKGEVAEAGEAPAKKAAIKKAPAKKAAPKKTEKKED" }, "paralog": false, "identity": 0.9829059829059829, "score": 415.0, "evalue": 1.09e-149, "description": "Internal stop codon at 28782. Nonsense mutation around 28782.", "psc": { "uniref90_id": "UniRef90_A0A328EG21", "product": "Small ribosomal subunit protein uS5", "uniref50_id": "UniRef50_A0A7W4E3Q4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EG21", "UniRef:UniRef50_A0A7W4E3Q4" ] }, "pscc": { "uniref50_id": "UniRef50_A0A7W4E3Q4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7W4E3Q4" ], "product": "Small ribosomal subunit protein uS5" } }, "id": "NKOCPLAKPK_1684", "locus": "GCA003260355_01666" }, { "type": "cds", "sequence": "contig_2", "start": 28780, "stop": 28953, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00333.26" ], "nt": "ATGTCTATGAGATCAGAAAAGGACAATAAGAGAGATGTAAGGAGAGAAAAGCCAAAGAAGGAGTTTGAAGAGCTTCTTTTGGAGGTAAGAAGAGTAACAAGAGTTACTACTGGAGGGAGAAGGCTTTCTTTCAGAGCAACAATCTTAGTTGGAAACAAAAAAGGAAAAATCTGA", "aa": "MSMRSEKDNKRDVRREKPKKEFEELLLEVRRVTRVTTGGRRLSFRATILVGNKKGKI", "aa_hexdigest": "46dbcc71cad0965a6ed0786b7737837c", "start_type": "ATG", "rbs_motif": "TTTA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00333.26", "name": "Ribosomal_S5", "length": 36, "aa_cov": 0.631578947368421, "hmm_cov": 1.0, "evalue": 7.261748563791285e-11, "score": 38.44490432739258, "start": 22, "stop": 57 } ], "seq_stats": { "molecular_weight": 6744.858300000002, "isoelectric_point": 11.228216743469236 }, "id": "NKOCPLAKPK_1685", "locus": "GCA003260355_01667" }, { "type": "cds", "sequence": "contig_2", "start": 28987, "stop": 29346, "strand": "-", "frame": 2, "gene": null, "product": "50S ribosomal protein L18", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBF7D18", "UniRef:UniRef100_A0A660MTW6", "UniRef:UniRef50_A0A2W4IKS9", "UniRef:UniRef90_A0A660MTW6" ], "nt": "ATGAGTTATTTGAAGAATAAACTCCAAGAAAAGATTAGCAAATACTTGAGAAGGAAGAATAAGGTAAATGCTAAAATCAAAGCATTGAATCCTGAATTCAGACTTTTGATTCAGAGGTCTAATCTTTATGTAAAAGCTCAGGTATTAGGAGCTGACGGAGTTGTTATTGCAACAACTACAGACAAAGCTGCTTCTGGAGCGACTAAAACTGAAAGAGCTGCTGCCGCAGGTGAAGCATTTGCTGCAGTGCTTAAAGAAAAAAAGGTTGAAAAGCTGACTTTTGATAGGAATGGATTCCTCTATCATGGGAGGATTAAAGCTTTCGCTGATGGACTTAGAAAAGGAGGTATTCAGTTTTAA", "aa": "MSYLKNKLQEKISKYLRRKNKVNAKIKALNPEFRLLIQRSNLYVKAQVLGADGVVIATTTDKAASGATKTERAAAAGEAFAAVLKEKKVEKLTFDRNGFLYHGRIKAFADGLRKGGIQF", "aa_hexdigest": "01c2674ca1d51cc7e9f419c67cefa8e9", "start_type": "ATG", "rbs_motif": "TTATT", "ups": { "uniparc_id": "UPI000DBF7D18", "uniref100_id": "UniRef100_A0A660MTW6", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBF7D18", "UniRef:UniRef100_A0A660MTW6" ] }, "ips": { "uniref100_id": "UniRef100_A0A660MTW6", "uniref90_id": "UniRef90_A0A660MTW6" }, "psc": { "uniref90_id": "UniRef90_A0A660MTW6", "product": "50S ribosomal protein L18", "uniref50_id": "UniRef50_A0A2W4IKS9" }, "pscc": { "uniref50_id": "UniRef50_A0A2W4IKS9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4IKS9" ], "product": "Large ribosomal subunit protein uL18" }, "genes": [], "id": "NKOCPLAKPK_1686", "locus": "GCA003260355_01668" }, { "type": "cds", "sequence": "contig_2", "start": 29383, "stop": 29928, "strand": "-", "frame": 2, "gene": "rplF", "product": "50S ribosomal protein L6", "db_xrefs": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "SO:0001217", "UniParc:UPI000DBF7E15", "UniRef:UniRef100_A0A328EBZ3", "UniRef:UniRef50_P0DOY8", "UniRef:UniRef90_A0A328EBZ3" ], "nt": "ATGTCAAAAATTGGTAAGAAGCCTCTTGTAATCCCTCAGGGAGTAGAAGTAGACTTGAAGGAAACAGCGGTACAGGTAAAAGGACCGAAAGGAACATTGGATCTTAATTTGCCATACGGAGTGAAAATTGAAAAAAATGAATCAGAATTAGTTTTTTCTATTCTTGATGACCAGTATAAGAATCTTTGGGGATTAGCAAGGACACTTGTTGCTAATATGGTTGAAGGAGTTTCTAACGGATATGAAAAGAAACTGCTTATCATTGGAGTAGGATACTCAGCAAAAGTTGAAGGAAAAACACTCGTGCTTTCTCTTGGATATGCTCACAAGGTAAACTTTCCAATTCCAGAGAGGATTGAAATAAGGACAGAACAAGATCCAAAAGGTAACACTGTAGTTTTTATCTCAGGAATTAGTAAAGAATTAGTTGGTGAGGTGGCTGCAAAGGTTAGAAGTATGAAAAAGCCTGAACCTTATAAAGGAAAGGGGATTAGATACTTTGATGAGTTTGTGAAGATTAAACCAGGTAAATCTGCGAAAAAATAA", "aa": "MSKIGKKPLVIPQGVEVDLKETAVQVKGPKGTLDLNLPYGVKIEKNESELVFSILDDQYKNLWGLARTLVANMVEGVSNGYEKKLLIIGVGYSAKVEGKTLVLSLGYAHKVNFPIPERIEIRTEQDPKGNTVVFISGISKELVGEVAAKVRSMKKPEPYKGKGIRYFDEFVKIKPGKSAKK", "aa_hexdigest": "1a0de00d5afced02844f3405f1fd014c", "start_type": "ATG", "rbs_motif": "TTTA", "ups": { "uniparc_id": "UPI000DBF7E15", "uniref100_id": "UniRef100_A0A328EBZ3", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBF7E15", "UniRef:UniRef100_A0A328EBZ3" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EBZ3", "uniref90_id": "UniRef90_A0A328EBZ3" }, "psc": { "uniref90_id": "UniRef90_A0A328EBZ3", "gene": "rplF", "product": "50S ribosomal protein L6", "uniref50_id": "UniRef50_P0DOY8", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843" ] }, "pscc": { "uniref50_id": "UniRef50_P0DOY8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P0DOY8" ], "product": "Large ribosomal subunit protein uL6" }, "genes": [ "rplF" ], "id": "NKOCPLAKPK_1687", "locus": "GCA003260355_01669" }, { "type": "cds", "sequence": "contig_2", "start": 29972, "stop": 30394, "strand": "-", "frame": 1, "gene": null, "product": "Small ribosomal subunit protein uS8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A288DIK3", "UniRef:UniRef90_A0A660MW17" ], "nt": "ATGAATCGTATCACTAAGATGAGTTATGTTAATGCACCAGTTCACGATTTGTTAATCAGGATCAAAAATGCATATATGGCGAGAAAAACAACCGTAGATGGAGTGGTTTTCTCGAAGTTTAAGGTAAATGTGCTTGAGCTCTTGAAACAATATGGGTTTGTGAAGGATTTTGAAGTGCAGGAAGTGAATGGAAAGAAATTTATTACTCTTCAGTTAAAAAAAGTAGTAGATCCTGTAAATGATATTCCTTCTGTAAAGTTTTTCTCTAAGCCATCTAGGCCTTGGTATGTTGGATACAAGGATTTAAAAACTGTTGCTGGAGGGAAGGGACTTGGAATTATTTCAACAAGTCAGGGGCTTTTGCCGGTGCATGTTGCTAAAGCAAAAAAGCTTGGTGGAGAATTGATTGCAGAAATCTATTAG", "aa": "MNRITKMSYVNAPVHDLLIRIKNAYMARKTTVDGVVFSKFKVNVLELLKQYGFVKDFEVQEVNGKKFITLQLKKVVDPVNDIPSVKFFSKPSRPWYVGYKDLKTVAGGKGLGIISTSQGLLPVHVAKAKKLGGELIAEIY", "aa_hexdigest": "d1132b9bed6fb7d458b3af85ec6fab4d", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A660MW17", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.993, "score": 277.0, "evalue": 1.65e-94, "valid": true, "product": "Small ribosomal subunit protein uS8", "uniref50_id": "UniRef50_A0A288DIK3" }, "pscc": { "uniref50_id": "UniRef50_A0A288DIK3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A288DIK3" ], "product": "Small ribosomal subunit protein uS8" }, "genes": [], "id": "NKOCPLAKPK_1688", "locus": "GCA003260355_01670" }, { "type": "cds", "sequence": "contig_2", "start": 30412, "stop": 30591, "strand": "-", "frame": 2, "gene": null, "product": "Small ribosomal subunit protein uS14", "db_xrefs": [ "KEGG:K02954", "SO:0001217", "UniRef:UniRef50_A0A7W4EBI2", "UniRef:UniRef90_A0A7W4EBI2" ], "nt": "ATGTTTGAAAAGAGGTTCGTGAAAGGGGAAAATCTTGAACAGCCTACAAAATATTATAACAGATGCAGACTTTGTGGAAGGTCCAAGGCATATATTAGAGATTTCGGAATTTGTAGAGTATGCCTTAGAAGGTATGCGAGAGAAGGTTTAATTATGGGACTTAGAAAAGCAAGTTGGTAA", "aa": "MFEKRFVKGENLEQPTKYYNRCRLCGRSKAYIRDFGICRVCLRRYAREGLIMGLRKASW", "aa_hexdigest": "18b32dab7c45c40098523c1406e96ae8", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A7W4EBI2", "query_cov": 1.0, "subject_cov": 0.8309859154929577, "identity": 0.966, "score": 124.0, "evalue": 4.83e-36, "valid": true, "product": "Small ribosomal subunit protein uS14", "uniref50_id": "UniRef50_A0A7W4EBI2", "kegg_orthology_id": "K02954" }, "pscc": { "uniref50_id": "UniRef50_A0A7W4EBI2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7W4EBI2" ], "product": "Small ribosomal subunit protein uS14" }, "genes": [], "id": "NKOCPLAKPK_1689", "locus": "GCA003260355_01671" }, { "type": "cds", "sequence": "contig_2", "start": 30659, "stop": 31183, "strand": "-", "frame": 1, "gene": "rplE", "product": "50S ribosomal protein L5", "db_xrefs": [ "GO:0003735", "GO:0005840", "GO:0006412", "KEGG:K02931", "SO:0001217", "UniParc:UPI000DBFA38D", "UniRef:UniRef100_A0A660MW09", "UniRef:UniRef50_A0A288DGP7", "UniRef:UniRef90_A0A660MW09" ], "nt": "ATGAAAAAATCTGAGTTGTTTGCAAAGCTTAAGGATCATTTGAACCTTAAAAACATCAATCAGGCTCCAAAAGTAGATAAAGTTATTGTTGCTATGGGAATTGGATCTTTGGTGACAAGAAAAGGACATAAAGATTTTGAAGAATTTGAAAGAAATCTGATTAGAATTACAGGTCAAAAGCCAGTATTGATAAAATCGAGAAAGGCTATTTCAAACTTCAAACTCAGGGAAGGAATGCCAGTAATGTTAAAAGTGACCTTAAGAGGAGAAAGAGCTTATGATTTCTTAGATAGATTCTGTAAGCTGGTGCTTCCCAGGGTAAGAGACTTTTCTGGAATATCCAAGAAGAACTTTGATCCAAAGGGAAATCTTAATCTTGGACTTCAGAATTATAATATCTTTCCTGAACTTGGTGTTGATGATGTAACACTTCCTATGGGAATTCAGCTTACAGTTGTAAGTACAGCAGGAAATCCAGAAGGAACTCAAGCATTACTTCAGGAGCTAGGATTTATCTTCAAATAA", "aa": "MKKSELFAKLKDHLNLKNINQAPKVDKVIVAMGIGSLVTRKGHKDFEEFERNLIRITGQKPVLIKSRKAISNFKLREGMPVMLKVTLRGERAYDFLDRFCKLVLPRVRDFSGISKKNFDPKGNLNLGLQNYNIFPELGVDDVTLPMGIQLTVVSTAGNPEGTQALLQELGFIFK", "aa_hexdigest": "ee490680edc35fdfdcbed81d1fdec75e", "start_type": "ATG", "rbs_motif": "TTTTT", "ups": { "uniparc_id": "UPI000DBFA38D", "uniref100_id": "UniRef100_A0A660MW09", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBFA38D", "UniRef:UniRef100_A0A660MW09" ] }, "ips": { "uniref100_id": "UniRef100_A0A660MW09", "uniref90_id": "UniRef90_A0A660MW09" }, "psc": { "uniref90_id": "UniRef90_A0A660MW09", "gene": "rplE", "product": "50S ribosomal protein L5", "uniref50_id": "UniRef50_A0A288DGP7", "kegg_orthology_id": "K02931", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_A0A288DGP7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A288DGP7" ], "product": "Large ribosomal subunit protein uL5" }, "genes": [ "rplE" ], "id": "NKOCPLAKPK_1690", "locus": "GCA003260355_01672" }, { "type": "cds", "sequence": "contig_2", "start": 31213, "stop": 31503, "strand": "-", "frame": 2, "gene": "rplX", "product": "50S ribosomal protein L24", "db_xrefs": [ "GO:0003735", "GO:0005840", "GO:0006412", "SO:0001217", "UniParc:UPI000DBF9688", "UniRef:UniRef100_A0A328EG78", "UniRef:UniRef50_A0A2G6CBT1", "UniRef:UniRef90_A0A328EG78" ], "nt": "ATGAAAAAATTGAGAAAAGGTGATCCAGTTATCGTGATTGCAGGAAAGGCAAAAGGTAGTGTTTCTGTAATAGAAGCTCTAGATGGAGATATGGTAACCGTAAAGGATGTAAATGTTGTAAAAAAAGCAGTAAAGGGACAGGGGTTTATAAAAAAGACCTTGCCACTCCATGTTTCTAATGTCGCATATTATCTTGCTGATCAGAAGAAAGCTACAAAAATTGGAATTGAACTTACAAAAGATGGTAAGAAGGTGAGAAAGGCGAAAGTTACTGGAAAAGTTATTGATTAG", "aa": "MKKLRKGDPVIVIAGKAKGSVSVIEALDGDMVTVKDVNVVKKAVKGQGFIKKTLPLHVSNVAYYLADQKKATKIGIELTKDGKKVRKAKVTGKVID", "aa_hexdigest": "72c6134ad404d5146f661fad6a4b92d2", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000DBF9688", "uniref100_id": "UniRef100_A0A328EG78", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBF9688", "UniRef:UniRef100_A0A328EG78" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EG78", "uniref90_id": "UniRef90_A0A328EG78" }, "psc": { "uniref90_id": "UniRef90_A0A328EG78", "gene": "rplX", "product": "50S ribosomal protein L24", "uniref50_id": "UniRef50_A0A2G6CBT1", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_A0A2G6CBT1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2G6CBT1" ], "product": "Large ribosomal subunit protein uL24" }, "genes": [ "rplX" ], "id": "NKOCPLAKPK_1691", "locus": "GCA003260355_01673" }, { "type": "cds", "sequence": "contig_2", "start": 31540, "stop": 31917, "strand": "-", "frame": 2, "gene": "rplN", "product": "50S ribosomal protein L14", "db_xrefs": [ "GO:0003735", "GO:0006412", "GO:0015934", "KEGG:K02874", "SO:0001217", "UniParc:UPI000DC04FA1", "UniRef:UniRef100_A0A328ECR1", "UniRef:UniRef50_A0A288DMN5", "UniRef:UniRef90_A0A328ECR1" ], "nt": "ATGTTGCAACAAGAATCGTTGGTTGTGGTAGCTGATAACACCCAAGCAAAAAAGGCAAAAATTATCAGAGTATTGAAGGGTTCTTACGGAAAAATAGCACATGTTGGAGATAGAGTTGTTGTTGCTATCAAAGATGCTGCACCTACTGCATCAGTGAAAAAGGGGCAAGTAGTTCAGGCAGTGGTAGTAAGAACAAAGAAAGAGATTGCTAGAAAAGATGGTACTTATATTAGATTTGCTGATAATGCAGTAGTTCTTTTGACTAAAGATGCGAAAGGAAATATGAATCCTATTGGTAAGAGGGTTTTTGGACCTGTAGCAAGAGAGCTGAGAGAAAAATATAGACCAATTACAAATATGGCAGAAGAAGTATTGTAA", "aa": "MLQQESLVVVADNTQAKKAKIIRVLKGSYGKIAHVGDRVVVAIKDAAPTASVKKGQVVQAVVVRTKKEIARKDGTYIRFADNAVVLLTKDAKGNMNPIGKRVFGPVARELREKYRPITNMAEEVL", "aa_hexdigest": "9d46052f1fa0ed8c5eea3b6b610014e8", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000DC04FA1", "uniref100_id": "UniRef100_A0A328ECR1", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DC04FA1", "UniRef:UniRef100_A0A328ECR1" ] }, "ips": { "uniref100_id": "UniRef100_A0A328ECR1", "uniref90_id": "UniRef90_A0A328ECR1" }, "psc": { "uniref90_id": "UniRef90_A0A328ECR1", "gene": "rplN", "product": "50S ribosomal protein L14", "uniref50_id": "UniRef50_A0A288DMN5", "kegg_orthology_id": "K02874", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015934" ] }, "pscc": { "uniref50_id": "UniRef50_A0A288DMN5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A288DMN5" ], "product": "Large ribosomal subunit protein uL14" }, "genes": [ "rplN" ], "id": "NKOCPLAKPK_1692", "locus": "GCA003260355_01674" }, { "type": "cds", "sequence": "contig_2", "start": 31943, "stop": 32194, "strand": "-", "frame": 1, "gene": "rpsQ", "product": "30S ribosomal protein S17", "db_xrefs": [ "GO:0003735", "GO:0005840", "GO:0006412", "SO:0001217", "UniParc:UPI000DC00AF7", "UniRef:UniRef100_A0A328EGQ0", "UniRef:UniRef50_A0A288DKG5", "UniRef:UniRef90_A0A328EGQ0" ], "nt": "ATGACAATTATCGGTGAAAAAAATATCAAAGAACTCGTTGGTGAGGTAGTAAGTGATAAAATGGATCAGACTTGTGTTGTTTTGGTGAAGTCTGTAAAAATGCATCCTATCTACAAGAAGAGATTTGTAGTGAGAAAAAAGTACTATGCTCACAACTCAGGGAATAAAGCAGTTCTTGGAGATACTGTAAAATTGAGATTGGTAAAACCAATTTCTAGACTCAAGAGATGGAATATTATTGAGATCTTATAG", "aa": "MTIIGEKNIKELVGEVVSDKMDQTCVVLVKSVKMHPIYKKRFVVRKKYYAHNSGNKAVLGDTVKLRLVKPISRLKRWNIIEIL", "aa_hexdigest": "dc6ebb4bfa8770069ae235bd7723e75e", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000DC00AF7", "uniref100_id": "UniRef100_A0A328EGQ0", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DC00AF7", "UniRef:UniRef100_A0A328EGQ0" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EGQ0", "uniref90_id": "UniRef90_A0A328EGQ0" }, "psc": { "uniref90_id": "UniRef90_A0A328EGQ0", "gene": "rpsQ", "product": "30S ribosomal protein S17", "uniref50_id": "UniRef50_A0A288DKG5", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_A0A288DKG5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A288DKG5" ], "product": "Small ribosomal subunit protein uS17" }, "genes": [ "rpsQ" ], "id": "NKOCPLAKPK_1693", "locus": "GCA003260355_01675" }, { "type": "cds", "sequence": "contig_2", "start": 32194, "stop": 32400, "strand": "-", "frame": 2, "gene": "rpmC", "product": "50S ribosomal protein L29", "db_xrefs": [ "GO:0003735", "GO:0005840", "GO:0006412", "SO:0001217", "UniParc:UPI000DBF5DD1", "UniRef:UniRef100_A0A328ECQ1", "UniRef:UniRef50_A0A328ECQ1", "UniRef:UniRef90_A0A328ECQ1" ], "nt": "ATGAAAGGAACTGGTTTATTGATGAAAGAGCTTGCTGGAAAGGATATCAAGGAACTTATAGCTATGAGAAAACAAGCTAGACACGAACTTTTTGAACTCAGAATGAAAAATAGTGTAAGGGCTCTTAAGGAAACTCATAAAATAGGAGATCTTAGAGTTAAGATTGCTAGGATCAATACCGTTTTACACTCTAAAGTGAATGCATAA", "aa": "MKGTGLLMKELAGKDIKELIAMRKQARHELFELRMKNSVRALKETHKIGDLRVKIARINTVLHSKVNA", "aa_hexdigest": "3ee50687b71746160efa5a485b5209b0", "start_type": "ATG", "rbs_motif": "TTCTA", "ups": { "uniparc_id": "UPI000DBF5DD1", "uniref100_id": "UniRef100_A0A328ECQ1", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBF5DD1", "UniRef:UniRef100_A0A328ECQ1" ] }, "ips": { "uniref100_id": "UniRef100_A0A328ECQ1", "uniref90_id": "UniRef90_A0A328ECQ1" }, "psc": { "uniref90_id": "UniRef90_A0A328ECQ1", "gene": "rpmC", "product": "50S ribosomal protein L29", "uniref50_id": "UniRef50_A0A328ECQ1", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328ECQ1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328ECQ1" ], "product": "Large ribosomal subunit protein uL29" }, "genes": [ "rpmC" ], "id": "NKOCPLAKPK_1694", "locus": "GCA003260355_01676" }, { "type": "cds", "sequence": "contig_2", "start": 32431, "stop": 32724, "strand": "-", "frame": 2, "gene": "rplP", "product": "50S ribosomal protein L16", "db_xrefs": [ "GO:0003735", "GO:0006412", "GO:0019843", "KEGG:K02878", "SO:0001217", "UniRef:UniRef50_A0A288GTS3", "UniRef:UniRef90_A0A660MTV2" ], "nt": "GTGACCAATAAGCAGTTGGAAGCAGCGAGAAAGGTTCTTGTAAGAGAAACAAGAAAGGTTGGTCAGCTTTGGATGAGGGTGTTTCCTGATGTTCCAATTACTAAGAAAGGACTTGAGATGCCGATGGGATCTGGAAAAGGAGATGTAGATATCTATGCTGCTCCAGTGAGGAAAGGAAAGGTTCTCTTTGAACTTTCTGGACTTTCAAGAGAATTGGCAGAAAAAGTCATTGTAAGTGCTGCGAAAAAATTGCCAATTAAAGCAAGATTTGTAGCTAAGGGTGAAATTAAGTAA", "aa": "MTNKQLEAARKVLVRETRKVGQLWMRVFPDVPITKKGLEMPMGSGKGDVDIYAAPVRKGKVLFELSGLSRELAEKVIVSAAKKLPIKARFVAKGEIK", "aa_hexdigest": "822637f068e62a7493f85359972abe5e", "start_type": "GTG", "rbs_motif": null, "genes": [ "rplP" ], "truncated": "3-prime", "pseudogene": { "start": 32431, "stop": 32850, "observations": { "mutation": [ 32760 ], "stop": [ 32760 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A660MTV2", "query_cov": 1.0, "subject_cov": 0.697841726618705, "identity": 0.99, "score": 185.0, "evalue": 7.52e-59, "gene_start": 1, "gene_end": 97, "reference_start": 43, "reference_end": 139, "reference_sequence": "MLLTPKKWKYRKQIVPSLKGKSSRGTGISFGEYGLKATTSGYVTNKQLEAARKVLVRETRKVGQLWMRVFPDVPITKKGLEMPMGSGKGDVDIYAAPVRKGKVLFELSGLSRELAEKVIVSAAKKLPIKARFVAKGEIK" }, "paralog": false, "identity": 0.9928057553956835, "score": 270.0, "evalue": 1.7e-94, "description": "Internal stop codon at 32760. Nonsense mutation around 32760.", "psc": { "uniref90_id": "UniRef90_A0A660MTV2", "gene": "rplP", "product": "50S ribosomal protein L16", "uniref50_id": "UniRef50_A0A288GTS3", "kegg_orthology_id": "K02878", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0019843" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A660MTV2", "UniRef:UniRef50_A0A288GTS3", "KEGG:K02878", "GO:0003735", "GO:0006412", "GO:0019843" ] }, "pscc": { "uniref50_id": "UniRef50_A0A288GTS3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A288GTS3" ], "product": "50S ribosomal protein L16" } }, "id": "NKOCPLAKPK_1695", "locus": "GCA003260355_01677" }, { "type": "cds", "sequence": "contig_2", "start": 32896, "stop": 32994, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAAAGTATGGAGTGCTTGGAATTAAGGTTTGGATTGAAAAAGGTGTTATTCCTTCAGCAAAGAAAAAAGGGAAGAAAACTCTGAATCTAGATTAA", "aa": "MTKYGVLGIKVWIEKGVIPSAKKKGKKTLNLD", "aa_hexdigest": "b170da7777a3546db22d542639abdb55", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3544.2988000000014, "isoelectric_point": 10.097054481506348 }, "id": "NKOCPLAKPK_1696", "locus": "GCA003260355_01678" }, { "type": "cds", "sequence": "contig_2", "start": 33097, "stop": 33537, "strand": "-", "frame": 2, "gene": "rpsC", "product": "30S ribosomal protein S3", "db_xrefs": [ "GO:0003735", "GO:0006412", "KEGG:K02982", "SO:0001217", "UniRef:UniRef50_A0A288DEY1", "UniRef:UniRef90_A0A660MTZ6" ], "nt": "ATGAGAGTTGGGATTATGAAATCACGACCATCAGAATGGTTTGCTAAGACAAGAAAAATGAATGCTGACTTTTTTGTTGAGGATATCCAAATCAGAGATTTCGTTGAAAAACAACTCGCAAGATCTGGGGTGGCTAAGATTGTGATTAGAAAGACACTTAAAGAAGGAGAGATTATTATTTTCGCTTCAAAAGTTGGTGTTCTTATGGGGAAAAATGGAGAAAGAATTAAGAAGTTAGAAGAAGATCTTAGTAAAAAATTTGGAAAGCCATTCAAAATTAATGTAAAAGAAGTAAGAACTCCTGAACTTTCTGCAAAGATTATGGCTGAATATGTTGCTTCTCAGCTTGAGGCGAGAATGCCTTATAGAAAAGTAGTAAAAAATGTGCTTGCAAAGGTGATGGAAAAGGGTGCAAATGGAATTAAAATCCAGATTGGATGA", "aa": "MRVGIMKSRPSEWFAKTRKMNADFFVEDIQIRDFVEKQLARSGVAKIVIRKTLKEGEIIIFASKVGVLMGKNGERIKKLEEDLSKKFGKPFKINVKEVRTPELSAKIMAEYVASQLEARMPYRKVVKNVLAKVMEKGANGIKIQIG", "aa_hexdigest": "df4d765b55eb86642030856072484f6f", "start_type": "ATG", "rbs_motif": null, "genes": [ "rpsC" ], "truncated": "both", "pseudogene": { "start": 32896, "stop": 33564, "observations": { "mutation": [ 33099, 33561 ], "stop": [ 33099, 33561 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A660MTZ6", "query_cov": 1.0, "subject_cov": 0.6576576576576577, "identity": 1.0, "score": 276.0, "evalue": 6.99e-93, "gene_start": 1, "gene_end": 146, "reference_start": 10, "reference_end": 155, "reference_sequence": "MGQKVNPVGMRVGIMKSRPSEWFAKTRKMNADFFVEDIQIRDFVEKQLARSGVAKIVIRKTLKEGEIIIFASKVGVLMGKNGERIKKLEEDLSKKFGKPFKINVKEVRTPELSAKIMAEYVASQLEARMPYRKVVKNVLAKVMEKGANGIKIQIGGRLGGADIARSEKFIDGRVSLQTFRSDIDYHYLQAMTKYGVLGIKVWIEKGVIPSAKKKGKKTLNLD" }, "paralog": false, "identity": 0.990990990990991, "score": 425.0, "evalue": 9.94e-154, "description": "Internal stop codon at 33099, 33561. Nonsense mutation around 33099, 33561.", "psc": { "uniref90_id": "UniRef90_A0A660MTZ6", "gene": "rpsC", "product": "30S ribosomal protein S3", "uniref50_id": "UniRef50_A0A288DEY1", "kegg_orthology_id": "K02982", "go_ids": [ "GO:0003735", "GO:0006412" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A660MTZ6", "UniRef:UniRef50_A0A288DEY1", "KEGG:K02982", "GO:0003735", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_A0A288DEY1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A288DEY1" ], "product": "Small ribosomal subunit protein uS3" } }, "id": "NKOCPLAKPK_1697", "locus": "GCA003260355_01679" }, { "type": "cds", "sequence": "contig_2", "start": 33574, "stop": 33912, "strand": "-", "frame": 2, "gene": "rplV", "product": "50S ribosomal protein L22", "db_xrefs": [ "GO:0003735", "GO:0006412", "GO:0015934", "KEGG:K02890", "SO:0001217", "UniParc:UPI000DBF70BE", "UniRef:UniRef100_A0A660MTV7", "UniRef:UniRef50_A0A288DC75", "UniRef:UniRef90_A0A660MTV7" ], "nt": "ATGAGTAATCAGGTTACAGCAACATTAAAATATGCACTTCTTTCCGATAGGAAGCTTAATCTCATTGCGGATCTTGTGAGAGGAAAAAGAGTGACTGCTGCATTGGATATGCTTGAATTTATGCCAAAAAAAGGTGCAAGAGTGCTTCATAAACTTATTAAATCAGCGGCGGCTAATGCTGTGAATAATAATAAACTTCCACTTGATCAGCTCTTTATTGAAAGGATTGAGGTGGGGAGGGGACCTAAGATTAAAAGGGTAAGGTTTGTTTCTAGGTCTAGGATTAGCCATTATGAAAAATATAGGGCATATGTGAAAGTTGTTTTAAATACTAAGTAG", "aa": "MSNQVTATLKYALLSDRKLNLIADLVRGKRVTAALDMLEFMPKKGARVLHKLIKSAAANAVNNNKLPLDQLFIERIEVGRGPKIKRVRFVSRSRISHYEKYRAYVKVVLNTK", "aa_hexdigest": "29e075179544dbc8df1a966c066e7761", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000DBF70BE", "uniref100_id": "UniRef100_A0A660MTV7", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBF70BE", "UniRef:UniRef100_A0A660MTV7" ] }, "ips": { "uniref100_id": "UniRef100_A0A660MTV7", "uniref90_id": "UniRef90_A0A660MTV7" }, "psc": { "uniref90_id": "UniRef90_A0A660MTV7", "gene": "rplV", "product": "50S ribosomal protein L22", "uniref50_id": "UniRef50_A0A288DC75", "kegg_orthology_id": "K02890", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015934" ] }, "pscc": { "uniref50_id": "UniRef50_A0A288DC75", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A288DC75" ], "product": "Large ribosomal subunit protein uL22" }, "genes": [ "rplV" ], "id": "NKOCPLAKPK_1698", "locus": "GCA003260355_01680" }, { "type": "cds", "sequence": "contig_2", "start": 33981, "stop": 34151, "strand": "-", "frame": 3, "gene": null, "product": "Small ribosomal subunit protein uS19", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2G6QTY0" ], "nt": "GTGATTAAAGTTTGGGATAGGGCTTGTCATATTGTTCCTGAATTTATTGGATATACCTTTGCTGTGCATAATGGAAAACAATTTGTAAGTGTGTATGTGACTGAAGATATGTTAGGACATAGATTGGGAGAGTTTGTTCCTACAAGAACTTTTAGAGGACATCCGTTCTAA", "aa": "MIKVWDRACHIVPEFIGYTFAVHNGKQFVSVYVTEDMLGHRLGEFVPTRTFRGHPF", "aa_hexdigest": "ed8852b8dbed554d76022ba69736a58e", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0038590574", "query_cov": 0.9464285714285714, "subject_cov": 0.8548387096774194, "identity": 0.679, "score": 86.3, "evalue": 3.04e-21, "valid": false, "gene": "rpsS", "product": "30S ribosomal protein S19", "uniref50_id": "UniRef50_A0A2G6QTY0", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015935" ] }, "pscc": { "uniref50_id": "UniRef50_A0A2G6QTY0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2G6QTY0" ], "product": "Small ribosomal subunit protein uS19" }, "genes": [], "id": "NKOCPLAKPK_1699", "locus": "GCA003260355_01681" }, { "type": "cds", "sequence": "contig_2", "start": 34404, "stop": 34835, "strand": "-", "frame": 3, "gene": "rplB", "product": "50S ribosomal protein L2", "db_xrefs": [ "GO:0003723", "GO:0003735", "GO:0006412", "GO:0015934", "GO:0016740", "KEGG:K02886", "SO:0001217", "UniRef:UniRef50_P60405", "UniRef:UniRef90_A0A7W4E3R1" ], "nt": "TTGCATTATATTGATGGGGAAAAGAGATATGTTTTGGCTTGGAAAGGTGCAAAAGTTGGTCAGGTGGTAATGAATGGTGTGAAAGGAGAAATCGCTCCTGGGAATAGAAAGCAGCTGAAGGATATTCCAGAAGGAATGGCAATCTTCAATCTTGAGGTGACTCCTTTCTCTGAGGGGAAGTTGATTAAGTCTGCTGGAGGATTTGCTACTTTAGCAGGTAGAGATGAGGCCTTAAAGGTTGTATTTGTGAAGATGCCTTCAGGAGAAGTGAGAAAGTTCAATGAAAACTGCTGGGCAACACTTGGTGAGGTTTCTAATGCTGATCATAAGAATGTTGAAATCGGTAAAGCTGGAAGGCAAAGGTGGCTTGGTAGAAAGCCAAGAGTGCTTGGAAAGAATATGAACCCTGTAGACCATCCGCTTGGAGGTTGA", "aa": "MHYIDGEKRYVLAWKGAKVGQVVMNGVKGEIAPGNRKQLKDIPEGMAIFNLEVTPFSEGKLIKSAGGFATLAGRDEALKVVFVKMPSGEVRKFNENCWATLGEVSNADHKNVEIGKAGRQRWLGRKPRVLGKNMNPVDHPLGG", "aa_hexdigest": "1aa4d1a80b1c8a5ce3962b75680a9abb", "start_type": "TTG", "rbs_motif": null, "genes": [ "rplB" ], "truncated": "both", "pseudogene": { "start": 34275, "stop": 35117, "observations": { "mutation": [ 34406, 34949, 34997 ], "stop": [ 34406, 34949, 34997 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A7W4E3R1", "query_cov": 1.0, "subject_cov": 0.5107142857142857, "identity": 0.8740000000000001, "score": 269.0, "evalue": 4.7e-89, "gene_start": 1, "gene_end": 143, "reference_start": 95, "reference_end": 237, "reference_sequence": "MAVKKYKPYTPSRRFMIGYDFSDLTQKRPEKSLTKTLGRTGGRNNQGRITSRFMAGGHKRLYRIIDFKGYNKIDIPARVAAVEYDPYRTARIVLLNYVDGEKRYVLGWKGATVGQVVMNGNKGDIAAGNRRQLKDIPEGMNIFNLEITPFSEGKLIKSAGSLATIAGRDEALKVVFVKMPSGEVRKFNENCWATIGELSNGDHKNVVIGKAGRQRWLGRKPRVLGKNMNPVDHPLGGGEAHSPIGSKRGPKSFSGKLVASGMKTRKGKKWSDKFIVSKRK" }, "paralog": false, "identity": 0.8964285714285715, "score": 516.0, "evalue": 4.24e-189, "description": "Internal stop codon at 34406, 34949, 34997. Nonsense mutation around 34406, 34949, 34997.", "psc": { "uniref90_id": "UniRef90_A0A7W4E3R1", "gene": "rplB", "product": "50S ribosomal protein L2", "uniref50_id": "UniRef50_P60405", "kegg_orthology_id": "K02886", "go_ids": [ "GO:0003723", "GO:0003735", "GO:0006412", "GO:0015934", "GO:0016740" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A7W4E3R1", "UniRef:UniRef50_P60405", "KEGG:K02886", "GO:0003723", "GO:0003735", "GO:0006412", "GO:0015934", "GO:0016740" ] }, "pscc": { "uniref50_id": "UniRef50_P60405", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P60405" ], "product": "Large ribosomal subunit protein uL2" } }, "id": "NKOCPLAKPK_1700", "locus": "GCA003260355_01682" }, { "type": "cds", "sequence": "contig_2", "start": 34995, "stop": 35117, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTGTAAAGAAGTATAAACCATATACGCCTTCCAGAAGATTTATGATTGGGTATGACTTTTCAGAGCTTACTAAGAAAAGACCTGAAAAATCATTAACTATGATGATTGGAAGGACATGA", "aa": "MAVKKYKPYTPSRRFMIGYDFSELTKKRPEKSLTMMIGRT", "aa_hexdigest": "5d83d9d29d3f5bdfcbe9d2044775d788", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4787.6935, "isoelectric_point": 10.284850883483884 }, "id": "NKOCPLAKPK_1701", "locus": "GCA003260355_01683" }, { "type": "cds", "sequence": "contig_2", "start": 35152, "stop": 35493, "strand": "-", "frame": 2, "gene": null, "product": "Large ribosomal subunit protein uL23", "db_xrefs": [ "SO:0001217", "UniParc:UPI000F0D84B4", "UniRef:UniRef100_A0A660MWL1", "UniRef:UniRef50_A0A660MWL1", "UniRef:UniRef90_A0A660MWL1" ], "nt": "ATGACATTTAAAGAGAAATTGCAGAGAAGGGCGAGAAAGAGTGTTGCACAAAAGGCTCTCGTAAAATTTAGTCCCTACGATATTATCCTTGCTCCTGTATTAACTGAGAAGACCTATAAACAGCAAGAGGTTGCTAATAAATATGTTTTCAAGGTACATAGTGATGCTAATAAAAATGATATTGATGCTGCTATTCAATATCTTTATAAGGTGACTCCTCTAAAGATTAATGTTGTAAATACAGCATTTAAAGGAAGAGAAAGAAGAAAGCTTGTAAAGAGGGCTTTTAAAAAAGCGATAGTGACTTTGGGAGAAAAGGAAAAGATTGATTTTGCTGTGTAA", "aa": "MTFKEKLQRRARKSVAQKALVKFSPYDIILAPVLTEKTYKQQEVANKYVFKVHSDANKNDIDAAIQYLYKVTPLKINVVNTAFKGRERRKLVKRAFKKAIVTLGEKEKIDFAV", "aa_hexdigest": "58a57f7f5e5258ecf60119f744da9706", "start_type": "ATG", "rbs_motif": "TTTTT", "ups": { "uniparc_id": "UPI000F0D84B4", "uniref100_id": "UniRef100_A0A660MWL1", "db_xrefs": [ "SO:0001217", "UniParc:UPI000F0D84B4", "UniRef:UniRef100_A0A660MWL1" ] }, "ips": { "uniref100_id": "UniRef100_A0A660MWL1", "uniref90_id": "UniRef90_A0A660MWL1" }, "psc": { "uniref90_id": "UniRef90_A0A660MWL1", "product": "Large ribosomal subunit protein uL23", "uniref50_id": "UniRef50_A0A660MWL1" }, "pscc": { "uniref50_id": "UniRef50_A0A660MWL1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MWL1" ], "product": "Large ribosomal subunit protein uL23" }, "genes": [], "id": "NKOCPLAKPK_1702", "locus": "GCA003260355_01684" }, { "type": "cds", "sequence": "contig_2", "start": 35524, "stop": 35778, "strand": "-", "frame": 2, "gene": "rplD", "product": "50S ribosomal protein L4", "db_xrefs": [ "GO:0003735", "GO:0006412", "GO:0022625", "SO:0001217", "UniRef:UniRef50_A0A288DBU4", "UniRef:UniRef90_A0A660MVZ9" ], "nt": "GTGGTTTCAGGATTGGATCTTGATACTATGGAGCCGAATACAAAGGCTGCTATAGCGCTTCTTTCTAAAATGGGGAATGGGGGTAAAAAAGTGCTCTTCGTTCTAGATAAAAAAGAAGATGGGGTTGTAAAGTCTTTTAGAAATATTGAGGGGGTGAAATACCTTTTGGTGGATTATCTTAATCCTTATGATCTAATGAACGCTGAGGCTATTGTGTTTTTGAAACCGGCACTTGAATTTGTAAATCAGAATTAG", "aa": "MVSGLDLDTMEPNTKAAIALLSKMGNGGKKVLFVLDKKEDGVVKSFRNIEGVKYLLVDYLNPYDLMNAEAIVFLKPALEFVNQN", "aa_hexdigest": "5a34927b3b662e984fcc68bb979bec5a", "start_type": "GTG", "rbs_motif": null, "genes": [ "rplD" ], "truncated": "3-prime", "pseudogene": { "start": 35524, "stop": 36078, "observations": { "mutation": [ 35781 ], "stop": [ 35781 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A660MVZ9", "query_cov": 1.0, "subject_cov": 0.40384615384615385, "identity": 0.988, "score": 165.0, "evalue": 4.4e-50, "gene_start": 1, "gene_end": 84, "reference_start": 125, "reference_end": 208, "reference_sequence": "MVYAVDVYDKNGKLASKVDLNPELFSDEIVNPSLIHEYLLLQQANARNVIASTKTRAEVAGSGRKLYKQKGTGNARVGDKNSPIRRHGGVAFGPTPERNFEKTMTKKARKLALNGLLTLKVKEGVVSGLDLDTMEPNTKAAIALLSKMGNGGKKVLFVLDKKEDGVVKSFRNIEGVKYLLVDYLNPYDLMNAEAIVFLKPALEFVNQN" }, "paralog": false, "identity": 0.9782608695652174, "score": 350.0, "evalue": 1.91e-125, "description": "Internal stop codon at 35781. Nonsense mutation around 35781.", "psc": { "uniref90_id": "UniRef90_A0A660MVZ9", "gene": "rplD", "product": "50S ribosomal protein L4", "uniref50_id": "UniRef50_A0A288DBU4", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0022625" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A660MVZ9", "UniRef:UniRef50_A0A288DBU4", "GO:0003735", "GO:0006412", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_A0A288DBU4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A288DBU4" ], "product": "Large ribosomal subunit protein uL4" } }, "id": "NKOCPLAKPK_1703", "locus": "GCA003260355_01685" }, { "type": "cds", "sequence": "contig_2", "start": 35779, "stop": 36150, "strand": "-", "frame": 2, "gene": "rplD", "product": "50S ribosomal protein L4", "db_xrefs": [ "GO:0003735", "GO:0006412", "GO:0022625", "SO:0001217", "UniRef:UniRef50_A0A288DBU4", "UniRef:UniRef90_A0A660MVZ9" ], "nt": "ATGGTGTATGCAGTTGATGTATATGATAAAAATGGGAAGCTTGCTTCAAAAGTTGATTTGAATCCCGAGCTCTTTTCTGATGAGATTGTAAGCCCTTCTCTTATTCATGAATATCTTCTCCTTCAGCAGGCGAACGCTCGTAATGTAATTGCCTCTACAAAAACAAGAGCTGAAGTTGCTGGATCTGGAAGAAAGCTTTACAAACAGAAAGGGACTGGAAATGCAAGAGTGGGGGATAAAAATTCTCCAATAAGAAGGCATGGTGGAGTTGCTTTTGGTCCAACAGCAGAGAGGAATTTTGAAAAGATAATGACTAAAAAGGCAAGAAAGCTGGCTCTTAATGGTCTTTTGACTTTGAAAGTGAAGGAGTGA", "aa": "MVYAVDVYDKNGKLASKVDLNPELFSDEIVSPSLIHEYLLLQQANARNVIASTKTRAEVAGSGRKLYKQKGTGNARVGDKNSPIRRHGGVAFGPTAERNFEKIMTKKARKLALNGLLTLKVKE", "aa_hexdigest": "3088ff4bddaa93ee4bf63136e845a2e9", "start_type": "ATG", "rbs_motif": null, "genes": [ "rplD" ], "truncated": "5-prime", "pseudogene": { "start": 35524, "stop": 36150, "observations": { "mutation": [ 35781 ], "stop": [ 35781 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A660MVZ9", "query_cov": 1.0, "subject_cov": 0.5913461538461539, "identity": 0.976, "score": 231.0, "evalue": 2.23e-75, "gene_start": 1, "gene_end": 123, "reference_start": 1, "reference_end": 123, "reference_sequence": "MVYAVDVYDKNGKLASKVDLNPELFSDEIVNPSLIHEYLLLQQANARNVIASTKTRAEVAGSGRKLYKQKGTGNARVGDKNSPIRRHGGVAFGPTPERNFEKTMTKKARKLALNGLLTLKVKEGVVSGLDLDTMEPNTKAAIALLSKMGNGGKKVLFVLDKKEDGVVKSFRNIEGVKYLLVDYLNPYDLMNAEAIVFLKPALEFVNQN" }, "paralog": false, "identity": 0.9807692307692307, "score": 397.0, "evalue": 2.28e-143, "description": "Internal stop codon at 35781. Nonsense mutation around 35781.", "psc": { "uniref90_id": "UniRef90_A0A660MVZ9", "gene": "rplD", "product": "50S ribosomal protein L4", "uniref50_id": "UniRef50_A0A288DBU4", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0022625" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A660MVZ9", "UniRef:UniRef50_A0A288DBU4", "GO:0003735", "GO:0006412", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_A0A288DBU4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A288DBU4" ], "product": "Large ribosomal subunit protein uL4" } }, "id": "NKOCPLAKPK_1704", "locus": "GCA003260355_01686" }, { "type": "cds", "sequence": "contig_2", "start": 36179, "stop": 36784, "strand": "-", "frame": 1, "gene": null, "product": "Large ribosomal subunit protein uL3", "db_xrefs": [ "KEGG:K02906", "SO:0001217", "UniParc:UPI000DC04E54", "UniRef:UniRef100_A0A328EG88", "UniRef:UniRef50_A0A660MUJ1", "UniRef:UniRef90_A0A660MUJ1" ], "nt": "ATGGCAAATAAAGGAGGAGTTGTGGTTGCAAAACAAGAGATGACTAAGATGTGGATTGGTGATAAATTTGTGGCGGTTACTCTTGCTAAGGTTCTTCCACAAGAAATTATTAGATATAAAACTCAGGAAAAAGATGGGTATTCTGCTGTAGTTGTTGGGGTTGAAAAAAAAGAACTCAATAAAGAAAAGGGGCAGAAGCTGATATATAGTATGGTGACAGAGTTTCCTGTAGATGAGGCTTTTGTTTCTGCGAACGAGGCTGGAAAGATTCTTGACGCTTCTTTGTTGGAAGGTGTATCTTCTCTTGCTGTGACTGGGATGACTAAAGGAAAGGGGTATCAGGGACTAGTGAAGAGGTGTAATGTTAAAGGAGGTCCTGCTACCCATGGGCACAAGTTTTCTAGAGCAGGTGGTTCTAAGGGTAATAGAAAGCCAAGAAGAACTATGAAGGGGCATCCTCATGCAGGACATATGGGTTCAGAGCAGCTTACTCTCAAAAGAATTCCTCTTTTGAATATCGTAGAAAAGGATGGCGTGAAGTTTTTGATTGTTAAAGGATCTTTGCCTGGTGCAAGAAACGGAAAGTTGAAATGTTTTGTAGAATAA", "aa": "MANKGGVVVAKQEMTKMWIGDKFVAVTLAKVLPQEIIRYKTQEKDGYSAVVVGVEKKELNKEKGQKLIYSMVTEFPVDEAFVSANEAGKILDASLLEGVSSLAVTGMTKGKGYQGLVKRCNVKGGPATHGHKFSRAGGSKGNRKPRRTMKGHPHAGHMGSEQLTLKRIPLLNIVEKDGVKFLIVKGSLPGARNGKLKCFVE", "aa_hexdigest": "9bf5302af4f280f1796b3db5a7d05ce9", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000DC04E54", "uniref100_id": "UniRef100_A0A328EG88", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DC04E54", "UniRef:UniRef100_A0A328EG88" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EG88", "uniref90_id": "UniRef90_A0A660MUJ1" }, "psc": { "uniref90_id": "UniRef90_A0A660MUJ1", "product": "Large ribosomal subunit protein uL3", "uniref50_id": "UniRef50_A0A660MUJ1", "kegg_orthology_id": "K02906" }, "pscc": { "uniref50_id": "UniRef50_A0A660MUJ1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MUJ1" ], "product": "Large ribosomal subunit protein uL3" }, "genes": [], "id": "NKOCPLAKPK_1705", "locus": "GCA003260355_01687" }, { "type": "cds", "sequence": "contig_2", "start": 36884, "stop": 37213, "strand": "-", "frame": 1, "gene": "rpsJ", "product": "30S ribosomal protein S10", "db_xrefs": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022627", "SO:0001217", "UniParc:UPI000DBFBBEC", "UniRef:UniRef100_A0A660MWT7", "UniRef:UniRef50_A0A288DKG2", "UniRef:UniRef90_A0A660MWT7" ], "nt": "ATGTCGGCTGTAAAAAAAGATCAGACTCCAAAGTTGAGAATTAAGTTAAAGAGTTATGATGTAAGAATGTTGGAAGCTTCTGTTTCTAAGGTTGTAGGACTTTTGGTAAAATCAGGTGCTCAGATTAAAGGACCAATTCCTTTGCCAAAGAAAAGGAAGGTATATACCGTGTTAAGATCAAACTTTAAGTTTAAAAAAGCAAGAGAGCAATTTGAAAGAATCTCTTATACAAGAGTCTTGGATGTTGTAGAGACTGGAGGGAAGACAATGGAGTATCTTCAGAATCTTTCAATTCCCGTAGGAGTGCTTGTAGATGTGAAGGTTTTCTAG", "aa": "MSAVKKDQTPKLRIKLKSYDVRMLEASVSKVVGLLVKSGAQIKGPIPLPKKRKVYTVLRSNFKFKKAREQFERISYTRVLDVVETGGKTMEYLQNLSIPVGVLVDVKVF", "aa_hexdigest": "caaaa9fc51b6ccb6eef30df37f456975", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000DBFBBEC", "uniref100_id": "UniRef100_A0A660MWT7", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBFBBEC", "UniRef:UniRef100_A0A660MWT7" ] }, "ips": { "uniref100_id": "UniRef100_A0A660MWT7", "uniref90_id": "UniRef90_A0A660MWT7" }, "psc": { "uniref90_id": "UniRef90_A0A660MWT7", "gene": "rpsJ", "product": "30S ribosomal protein S10", "uniref50_id": "UniRef50_A0A288DKG2", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022627" ] }, "pscc": { "uniref50_id": "UniRef50_A0A288DKG2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A288DKG2" ], "product": "Small ribosomal subunit protein uS10" }, "genes": [ "rpsJ" ], "id": "NKOCPLAKPK_1706", "locus": "GCA003260355_01688" }, { "type": "cds", "sequence": "contig_2", "start": 37305, "stop": 38477, "strand": "-", "frame": 3, "gene": "tuf", "product": "elongation factor Tu", "db_xrefs": [ "GO:0003746", "GO:0005525", "GO:0006414", "SO:0001217", "UniParc:UPI000DBF5750", "UniRef:UniRef100_A0A328EGR2", "UniRef:UniRef50_Q9P9Q9", "UniRef:UniRef90_A0A328EGR2" ], "nt": "ATGGCTACTAAACCACACGTAAATGTTGGAACTATTGGTCACGTTGATCACGGTAAGACAACATTGACTGCTGCTCTTACAGCTGTTGCTGCTACAAAAGGGTATGCTAAATTTACAGCGTATGATCAAATTGATAACGCTCCAGAAGAGAAGGCAAGAGGTATTACTATTGCTTCTAGACATGTAGAATATGAAACTGACAACAGACACTATGCTCATATTGACTGTCCAGGGCATGCTGACTACGTAAAGAACATGATCGTAGGTGCTGCTCAGATGGATGGAGCTATTCTTCTTTGTTCTGCTGTTGATGGTCCTATGCCTCAGACTAGGGAACATATTCTTTTGGCTAAGCAAGTTAATGTGCCAAAGATTGTTGTTTTCTTGAATAAATGTGATATGGTAGATGATCCTGATATGATTGATCTTGTAGAAGAAGAAATCAGAGAGATTCTAAAGAAGAATGGTTTTGATGAAAATTGTCCTATTATTAGAGGATCAGCTTTGAAGGCTGTTGAAAATCCTACTGATGAGGCTGCTGCAAAACCTATTTGGGAGTTGTTTGATGCTTTGGATTCTTACATTCCAGTTCCTGAAAGAGATAATGATAAGCCTTTCTTGATGCCAGTTGAAGATGTGTTCTCTATTAAAGGTAGAGGAACTGTGGCAACAGGTAGAATTGAAAGAGGTATTGTAAAAATGAATGATGAGCTTGAACTTCTTGGTCTTGGTGCTGATCCTTTGAAAACTGTTGTTACAGGTATTGAAATGTTCCACAAGGCGTGGGATCAGGCAGAGTCTGGTATGAATGTTGGGCTTTTGCTTAGAGGTGTAGACAAGGATGCTATTGAAAGAGGACAGGTGCTTTGTAAGCCAGGTACTGTGAAGACTTTCAAAAAGTTTGAAGCTGAAGTGTATGTACTTAAAAAGGAAGAAGGTGGAAGACATACTCCATTTATGAGTGGATATAGGCCTCAATTCTTCATGAGAACAACGGATGTTACCGGAAATATTGAACTTGCTCCTGGTGTAGAAATGATTATGCCTGGTGATAATGCAAAGATTATTGTTGAACTTATCTATCCTGTAGCTATGGAAGAGGGTTTGAGATTTGCAATTAGAGAAGGAGGTAGAACGGTTGGTGCTGGAGTTGTTACTAAAGTACTTGAATAA", "aa": "MATKPHVNVGTIGHVDHGKTTLTAALTAVAATKGYAKFTAYDQIDNAPEEKARGITIASRHVEYETDNRHYAHIDCPGHADYVKNMIVGAAQMDGAILLCSAVDGPMPQTREHILLAKQVNVPKIVVFLNKCDMVDDPDMIDLVEEEIREILKKNGFDENCPIIRGSALKAVENPTDEAAAKPIWELFDALDSYIPVPERDNDKPFLMPVEDVFSIKGRGTVATGRIERGIVKMNDELELLGLGADPLKTVVTGIEMFHKAWDQAESGMNVGLLLRGVDKDAIERGQVLCKPGTVKTFKKFEAEVYVLKKEEGGRHTPFMSGYRPQFFMRTTDVTGNIELAPGVEMIMPGDNAKIIVELIYPVAMEEGLRFAIREGGRTVGAGVVTKVLE", "aa_hexdigest": "392f88b715e232706fc88479960da81e", "start_type": "ATG", "rbs_motif": "TTATT", "ups": { "uniparc_id": "UPI000DBF5750", "uniref100_id": "UniRef100_A0A328EGR2", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBF5750", "UniRef:UniRef100_A0A328EGR2" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EGR2", "uniref90_id": "UniRef90_A0A328EGR2" }, "psc": { "uniref90_id": "UniRef90_A0A328EGR2", "gene": "tuf", "product": "elongation factor Tu", "uniref50_id": "UniRef50_Q9P9Q9", "go_ids": [ "GO:0003746", "GO:0005525", "GO:0006414" ] }, "pscc": { "uniref50_id": "UniRef50_Q9P9Q9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9P9Q9" ], "product": "Elongation factor Tu" }, "genes": [ "tuf" ], "id": "NKOCPLAKPK_1707", "locus": "GCA003260355_01689" }, { "type": "tRNA", "sequence": "contig_2", "start": 38713, "stop": 38786, "strand": "-", "gene": "trnP", "product": "tRNA-Pro(tgg)", "amino_acid": "Pro", "anti_codon": "tgg", "score": 70.9, "nt": "CGGGAATTAGCTCAGATGGCTAGAGCGCTTGGTTTGGGACCAAGAGGTCGCGAGTTCGAGTCCCGCATTCCCGA", "db_xrefs": [ "SO:0000268" ], "anti_codon_pos": [ 38768, 38770 ], "id": "NKOCPLAKPK_35", "locus": "GCA003260355_01690" }, { "type": "tRNA", "sequence": "contig_2", "start": 38795, "stop": 38871, "strand": "-", "gene": "trnI", "product": "tRNA-Ile(gat)", "amino_acid": "Ile", "anti_codon": "gat", "score": 79.1, "nt": "GGGCGTGTAGCTCAGGCGGTTAGAGCGCACCTCTGATAAGGGTGAGGTCGGTGGTTCGAGTCCACCCACGCCCACCA", "db_xrefs": [ "SO:0000263" ], "anti_codon_pos": [ 38835, 38837 ], "id": "NKOCPLAKPK_34", "locus": "GCA003260355_01691" }, { "type": "tRNA", "sequence": "contig_2", "start": 38895, "stop": 38969, "strand": "-", "gene": "trnF", "product": "tRNA-Phe(gaa)", "amino_acid": "Phe", "anti_codon": "gaa", "score": 81.1, "nt": "GCCAAGGTAGCTCAGTGGTAGAGCAGAGGCCTGAAAAGCCTTGTGTCGGCAGTTCGATTCTGCCTCTTGGCACCA", "db_xrefs": [ "SO:0000267" ], "anti_codon_pos": [ 38935, 38937 ], "id": "NKOCPLAKPK_33", "locus": "GCA003260355_01692" }, { "type": "rRNA", "sequence": "contig_2", "start": 39471, "stop": 40969, "strand": "-", "gene": "rrs", "product": "16S ribosomal RNA", "coverage": 0.9778212654924984, "score": 1339.5, "evalue": 8.8e-42, "db_xrefs": [ "GO:0005840", "GO:0003735", "RFAM:RF00177", "KEGG:K01977", "SO:0001000" ], "nt": "TTTTTGAAGAGTTTGACCCTGGCTCAGGATGAACGCTAGCGAAATGCCTAACACATGCAAGTCGAACGGATCTTGCGTCGTAGCAATACGTAGTAAGATTAGTGGCAAACGAGCGAGTAACGCGTGCTTAACTTGCCCCGAAGATGGGTATAACTACAGAGATGTAGCTAATTCCCAATATGCTCACGATGGTGAAATTCCAATGTGAGGAAAGATTTATCGCTTCGGGAGAGTGGTGCGTCCTATCAGGTAGTTGGTAAGGTAATGGCTTACCAAGCCTATGACGGGTAACCGGTCTGAGATGATGTCCGGTCGCGATGGGACTGAGATACGGCCCATACTCCTACGGGAGGCAGCAGTGGGGAATCTTGCACAATGGACGAAAGTCTGATGCAGCAATTTCGCGTGAAGGATGAAGCATTACGGTGTGTAAACTTCTTTTTTGGCAGAAGACGAATGACGGTATGTCAAGAATAAGAGACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTCTCAAGCGTTGTCCGGATTTACTGGGCGTAAAGTGTCCGTAGTCTGAATTGTAAGTCTGTTTTCAAATCCTACGACTCAATCGTAGAAAGGGAGTGGATACTGCAATTCTGGAAGTATCTGGGGGTTAGTGGAATTTCCGGTGGAGCGGTGAAATGCGTTGATATCGGAAGGAACGCCGAAAGCGAAAGCAGCTAACTACAGAATACTTGACGATGAGGGACGACAGTTCGGGTAGCAAACAGGATTAGATACCCTGGTAGTCCGAACCGTAAATTATGCTTGCTAGATGTTTTTGTCAATTTATTGGCAGGAGTGTCGTAATCTAACGAGTTAAGCAAGTCGCCTGGGTAGTATATTCGCAAGAATGAAACTCAAAGGGATAGGCGGGGGAACACACAAGCAGTGGATTATCTAGATTAATTGGATAATAAGCCAAGAATCTTACCTAGGATTGACATGTATTGTGTCTGCGGTGAAAGTCGCATATCCGTAGCAATACGGAGCTTTACACAGATGGTGCATGGTCGTCGTCAGCTCGTGCCGCAAGGTGTCTAGTTAAGTCTGGAAACGAGCGCAACCCTCATGCTTAGTTAGTATGTCTAAGCAGACTGCTCGGGTAACCGAGAGGAAGGAGAGGATGACGTCAGATCCTCATGCCTCTTACACCTAGGGCCTCATAGATAATACAATGGGTAGGTACAGCGAGAAGCAAGACCGCGAGGTGGAGCAAATCTTTAAAACTACCCCTAGTTCGGATTGTAGTCTGGAACTCGACTACATGAAGTTGGAATTGCTAGTAATGGCAGATCAGCCATGCTGCCGTGAATATGTCCCTGTTCCTTGCACTCACCGCCCGTCAAACCATGGGAGCTGAGGTTCTTTGAAGACTCATTATATGGGTTAAGGAGGAAATCAGTGACTGGGGTTAAGTCGTAACAAGGCATCCCTACCGGAAGGTGGGGATAGACCACCATTCTA", "id": "NKOCPLAKPK_42", "locus": "GCA003260355_01693" }, { "type": "rRNA", "sequence": "contig_2", "start": 41039, "stop": 41155, "strand": "-", "gene": "rrf", "product": "5S ribosomal RNA", "coverage": 0.9831932773109243, "score": 69.9, "evalue": 1e-11, "db_xrefs": [ "GO:0005840", "GO:0003735", "RFAM:RF00001", "KEGG:K01985", "SO:0000652" ], "nt": "GGTAGTGCTGATGCGGTATATGGAAACACCTGTTCCCATTCCGAATACAGAAGTTAAGCATATACTGGTCGATGATACTGGTTCTATCAGGGCTGGGAAAGTAGGTTAGTGCTACCA", "id": "NKOCPLAKPK_43", "locus": "GCA003260355_01694" }, { "type": "rRNA", "sequence": "contig_2", "start": 41274, "stop": 44213, "strand": "-", "gene": "rrl", "product": "23S ribosomal RNA", "coverage": 1.005128205128205, "score": 2426.2, "evalue": 1.5e-05, "db_xrefs": [ "GO:0005840", "GO:0003735", "RFAM:RF02541", "KEGG:K01980", "SO:0001001" ], "nt": "GATAAATCTATTTAAAAGCAATAAAAGGATGCCTTGGGTAAAAAAGAGGAAGAAGGACGTAGCTGGCTGCGATAAGTCTTGATGAGATGCCAACAATCGCTTGGAGTCAAGAATTTCCGAATGGGGCAACCTATTCTCTATAGGAGAATACAAAGTTGACAATACTCGGTGAAGTGAAATATCTCAGTAACCGAAGGAAAAGAAATCGTAATGAGATTCCCTTAGTAGTGACGAGCGAACAGGGAAGAGCCCAAACTCTATTAGTGTAAGCTTCTAGGCGTTGCTAATAAGAGGGTTGTAGGATCGTCCGGGAAGAGCTAGAACTCTTTGGATGGAGAGATGTTGTATTAGGAGAAGCATTTGGATGATGCACCATAGAAGGTTAAAGTCCCGTATCTGAAAATATGACACCACATCGTGACGACACCTGAGTAATATCAGACACGAGAAATCTGGTATGAATCTGCCCAGACCACTGGGTAAGGCTAAATACTTTTTTACACCGACAGCGAACAGTACCGTGAGGGAACGGTGAAAAGAACCCCTGGTAGGGGAGTGAAATAGAACCTGAAATTTATTGCTTACAATTGAGTGGGAGCCGCAAGGTGACCACGTACCTATTGACGAATGGGTCAGCGAGTTATTAGTAGTAGCGAGGTTAAGCCAGTTATAGGCGGAGCCGAAGGGAAACCGAGTCTGAATAGGGCGCTTTAGTTATTATTAATAGACCCGAAGCGGTATGAGCTAGACATGGGCAGGTTGAAGGCATAGGAAACTATGCTGGAGGACCGAACGGGTTGGTGCTGCAAAACCTTCCGATGACCTGTGTTTAGGAGTGAAAAGCTCATCGAATACCGTGATAGCTGGTTCTCTCCGAAATGCATTTAGGTGCAGCCTGCATTTTGAGATCTTGAAGAGGTAGAGCTCTGGAAAGGCTAGGGGTGTCGAGAGACATTACCAAACCTTACTAAACTTCGAATGCTTCAAGGAATATTGCAGAGTGGGACTACGGGAGCAAGTTTCGTAGTCAAAAGGATAACAGTCCAGACTTCTGATTAAGGTCCCTAAAGTAATGCTAAGTGGAAAAAGAGGTGTCTTTACTTATACATCCAGGAGGTTTGCCTAGAAGCAGCAATCCTTTAAAGAAAGCGTAACAGCTCACTGGACTAGCGAAGATGCGCTGAAGATGTAACGGGGCTAAGCATTATACCGAAATCGAAGACCAGTGTAGTAATACACTGTGTGGTAGGAGAGTGTTCTTACAGGATGAAGCTACATCGCGAGATGTGGTCGACTAGTAAGAAGTAAAAATGCTGACATGAGTAACGCTTATTGATGAAAATTCAATATATCGAAACCCTAAGGATTCCTACGCAACGTTCATCGTCGTAGGGTTAGTCGGGACCTAAGGCTTATCCAGAAGTGGAGAGTCGATGGATAACTGGTTAATATTCCAGTACTTATATAAAGAGTGATGGAGTAACGCATTGAGATTTGTGAGAGCTCATTAAGGATTTGAGTCGAAAGAGTGAGGTTTTTATAACCCTAGGTTATAAAGGTAGTTGCAGGAAAATCCACAGCTATTGATACTGAGCTTGATCGGAATTTGATTCCTCGGAATTAGAAATTCTCATCGTTTTGGTGCCGAGAAAAACTTCTAAACTTAATCTTTATGTAACCCGTACTCAGATCAGACACTTGTGGGGTGGTAGAGAATACTAAGATAATCGAGATAATTAATGGGTAAGGAACTTGGCAAATTAGCGGACGTACTTTCGAAATAAGTCCTGTCCCGAGCAATCGGGATCGCAGCGAAAGAGATGGGGGGAACTGTTTATCACAAACACAGCTCGTTGCTAACTCGTAAGAGGATGTATAACGGGTGATGCCTGTCCAATGCCAGAATGTTATGCTCTCTTGTGCAAGCTCGAGGGTTAAGCACTGGTGAATGACGGCCGTAACTATGACGGTCCTAAGGTAGCGAAATTCCTTGTCAGGTAAGTTCTGACGCGCATGAATGGCATAATCATCCCCATGCTGTCTTACCCTTAAACTCGGTGAAATTACATTCCCGGTAAAAATGCCGGGTAGTCACAGTTAGACGGAAAGACCCTGTGAAGCTTTACTGTAGATTGATATTGGATTGTAGCATGTCTTGCTCAGTATAGGTGGGAGGCTTTGATGTTTCAACTTCGGTTGGGGCGGAGCCAATAGTGAGATACCACCCAAGATATGTAGCAATTCTAATCCCTAGGTTGGGAGACAGTTTCAGTTGGACAGTTTACCTGGGGCGGGTGCCTCCTAAAAAGTAGCGGAGGCGTGCAAAGGTTTGCTTACTACGGATGGAAACCGTGGTGAATGTGTATTCGCATAAGCAAGCTTGACTGTGAGACTGACAGGTCGAGCAGATACGAAAGTAGGCGAAAGTGATCCGGATCTTCCAAGTGGAAGGGGATCCGCTCAACGGATAAAAGTTACTCCGGGGATAACAGGCTAATGGAATCTAAGCGTTCACAGCGACGATTCCGTTTGGCACCTCGATGTCGACTCGTCGCATCCTGGGGCTGTAGAAGGTCCCAAGGGTTGGGTTGTTCACCCATTAAAGCGGGACGCGAGTTGGGTTCAGAACGTCGTGAGACAGTTCGGTCCCTATCTACTGTGACCGTAGGATATTTGAGAGATGCTGACCCTAGTACGAGAGGACCGGGTTGGACGAGCCTCTGATAGTACCAGTTGTTCCGCCAGGAGCACCGCTGGATAGTTAAGCTCGGAAAAGGTAACCGCTGAAAGCATCTAAGTGGGAAACTTGTCTCAAGATTAGATATCCCTCTTCTTTATGAAGGTAAGACCGCTGGTAGACTACCAGTTTAATAGGCATTAGGTGTAAGTCCTGTAAGGGATTGAGCTAAGATGTACTAATTGGTCGAGCGGGTTTATCTT", "id": "NKOCPLAKPK_44", "locus": "GCA003260355_01695" }, { "type": "cds", "sequence": "contig_2", "start": 45178, "stop": 45399, "strand": "+", "frame": 1, "gene": null, "product": "OBG-type G domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1G2UIK3" ], "nt": "ATGAAAATTGGAATTGTTGGATTACCAAATGTTGGGAAGTCTACCTTGTTTAATGCTTTAACAAAGTCCTATGCTGCTCCTGCTGAGAATTTCCCCTTCTGCACTATAGAACCAAATATTGGGATTGTAGACGTTAAAGACCCTAGAGTTGAAGCTCTGGCAGAGATGTCTAAAAGCCAAAAAATTGTCTACGCTAATACCGAATTTGTGGACATTGCCTGA", "aa": "MKIGIVGLPNVGKSTLFNALTKSYAAPAENFPFCTIEPNIGIVDVKDPRVEALAEMSKSQKIVYANTEFVDIA", "aa_hexdigest": "53e58928321e11dd9b8740ae65fa55f3", "start_type": "ATG", "rbs_motif": "TTTTT", "psc": { "uniref90_id": "UniRef90_A0A1G2UIK3", "query_cov": 0.9863013698630136, "subject_cov": 0.9473684210526315, "identity": 0.6809999999999999, "score": 97.8, "evalue": 2.37e-25, "valid": false, "product": "OBG-type G domain-containing protein", "uniref50_id": "UniRef50_A0A1G2UIK3" }, "pscc": { "uniref50_id": "UniRef50_A0A1G2UIK3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1G2UIK3" ], "product": "OBG-type G domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_1708", "locus": "GCA003260355_01696" }, { "type": "cds", "sequence": "contig_2", "start": 45475, "stop": 46110, "strand": "+", "frame": 1, "gene": "ychF", "product": "redox-regulated ATPase YchF", "db_xrefs": [ "GO:0005524", "GO:0005525", "GO:0016887", "SO:0001217", "UniRef:UniRef50_A0A839JVT2", "UniRef:UniRef90_A0A328EG41" ], "nt": "GTGCAAGTGCTAAGATATTTTAAGGACAGTGATGTCGTCCATGTGGAAGGCGGAGTTGACCCTCTAAGAGATAGAGAGATCATCAATACTGAACTTATTTTTTCCGATCTTGAACAAATAGAAAAAACACTTCCGAATCTTCAAAAGAAAGCAAAGCTGGCTCAAAATAAGGACGATAAAAAGTCCGCAGAAATCCTCGAAAAAATACAAAAAGTCCTCTTGGAAGGAAGACTGGCAAACACCTTGAGAGAAGAGTTTGCGGAGGAGGAGCTCAAATTGATTAGGTCCTATAATTTTCTGACCCTAAAACCTTTTGTCTATGCACTGAATATTTCTCAGGAGGATATTCAAAATGCTGAAGTTCTGAAGAAGGAATTTGAAGAAAAACTCCAAGCTCCTGTTGCGATCGTCTGTGTCAAGATTGAATCCGAGATGATGGAACTTTCCAATGAAGAAAAATCTGAATTTCTGACTGAGCTTTTGGAACTCCAACAGGGAGCCAAGATTCCCACTCTGGATGATTTAATTTCCCTCGCCTTCAAACAGCTCGGACTTATGTACTATTTCACGACAGGAGAGAAAGAAACTAAAGCTTGGACCATCCCACTTAATTCTACTGCACCGCAGGCAGCCTGA", "aa": "MQVLRYFKDSDVVHVEGGVDPLRDREIINTELIFSDLEQIEKTLPNLQKKAKLAQNKDDKKSAEILEKIQKVLLEGRLANTLREEFAEEELKLIRSYNFLTLKPFVYALNISQEDIQNAEVLKKEFEEKLQAPVAIVCVKIESEMMELSNEEKSEFLTELLELQQGAKIPTLDDLISLAFKQLGLMYYFTTGEKETKAWTIPLNSTAPQAA", "aa_hexdigest": "1aed21ecccea3494d2501c44e6ddcdb6", "start_type": "GTG", "rbs_motif": null, "genes": [ "ychF" ], "truncated": "both", "pseudogene": { "start": 45178, "stop": 46284, "observations": { "mutation": [ 45397, 46108 ], "stop": [ 45397, 46108 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EG41", "query_cov": 1.0, "subject_cov": 0.5733695652173914, "identity": 0.986, "score": 391.0, "evalue": 6.47e-135, "gene_start": 1, "gene_end": 211, "reference_start": 100, "reference_end": 310, "reference_sequence": "MKIGIVGLPNVGKSTLFNALTKSYAAPAENFPFCTIEPNIGIVDVKDPRVEALAEMSKSQKIVYANTEFVDIAGLVKGASKGEGLGNKFLSHIREVDTIVQVLRYFKDSDVVHVEGGVDPLRDREIINTELIFSDLEQIEKTLPNLQKKAKLAQNKDDKKSAEILEKIQKVLLEGRLANTLREELAEEELKLIRSYNFLTLKPFVYALNISQEDIQNAEALKKEFEEKLQAPVAIVCVKIESEMMELSNEEKSEFLTELLELQQGAKIPTLDDLISLAFKQLGLMYYFTTGEKETKAWTIPLNSTAPQAAGAIHSDFERGFIKAEVINTEKLLESGSWAKAREKGLLRLEGKDYIVQDGDVMIFKFNV" }, "paralog": false, "identity": 0.9891304347826086, "score": 702.0, "evalue": 8.68e-260, "description": "Internal stop codon at 45397, 46108. Nonsense mutation around 45397, 46108.", "psc": { "uniref90_id": "UniRef90_A0A328EG41", "gene": "ychF", "product": "redox-regulated ATPase YchF", "uniref50_id": "UniRef50_A0A839JVT2", "go_ids": [ "GO:0005524", "GO:0005525", "GO:0016887" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EG41", "UniRef:UniRef50_A0A839JVT2", "GO:0005524", "GO:0005525", "GO:0016887" ] }, "pscc": { "uniref50_id": "UniRef50_A0A839JVT2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A839JVT2" ], "product": "Ribosome-binding ATPase YchF" } }, "id": "NKOCPLAKPK_1709", "locus": "GCA003260355_01697" }, { "type": "cds", "sequence": "contig_2", "start": 46339, "stop": 46623, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGAGGACAAATCAAGAGTGTTATTCTAGAAGGGCTGGTGAAAACTCAGCAAAGCATTAGTTTTGAAATCTGTAAGGCAACACTCGGGCAGCTACAACGTGTTTATCAAAAGCCAATATTCCAGGTTGGGGCTGTTTTTGCAATGTATTTGCTCTTCTACGGAGTGGTAAGATTGGTGGTGTGGATTCTTACTATTATTGCTTATGTTGTATTCCTCGCAATAAGACTATTCGGCCACTATTCTATAGAGAAAAAACTTGAAGAAGTAGAAGAAGTGCTCTAA", "aa": "MGGQIKSVILEGLVKTQQSISFEICKATLGQLQRVYQKPIFQVGAVFAMYLLFYGVVRLVVWILTIIAYVVFLAIRLFGHYSIEKKLEEVEEVL", "aa_hexdigest": "71bdd08e511b28f1df067cc480d83250", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10725.756399999997, "isoelectric_point": 8.78783054351807 }, "id": "NKOCPLAKPK_1710", "locus": "GCA003260355_01698" }, { "type": "cds", "sequence": "contig_2", "start": 46918, "stop": 47241, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAACAATATCTTCTTCTCTATCTTCATCTCACAAAAAAAGCTATCACCTTCCAAGGCTCTGCTTTTTCTTAGTTTTTTCTCTCATTACCTATGGATTTTTCCTTCGAAAGCTTTTGTGGGAAAGGCAGGTTCTTTGGGCAGTAGCAGCCTTTTGTGTCCTCTTTATTGGCTTAATATTCCTTGCTATAGATCTTAGGAGGGTAAAAAAGATAAAAATCTGGCTTCCAATACTTATTTTAGGAGCCGTTCTTCAGTTGATAATACGAAATGCTCCACGACAATTAATGATAGCTATGTGGCTTTCGCTGTTTGCAATCTGA", "aa": "METISSSLSSSHKKSYHLPRLCFFLVFSLITYGFFLRKLLWERQVLWAVAAFCVLFIGLIFLAIDLRRVKKIKIWLPILILGAVLQLIIRNAPRQLMIAMWLSLFAI", "aa_hexdigest": "253be7477ee14bcc73650faf90c258ea", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12481.227799999982, "isoelectric_point": 10.710729026794432 }, "id": "NKOCPLAKPK_1711", "locus": "GCA003260355_01699" }, { "type": "cds", "sequence": "contig_2", "start": 47245, "stop": 47652, "strand": "-", "frame": 2, "gene": null, "product": "Prepilin type IV endopeptidase peptidase domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MZ43", "UniRef:UniRef90_A0A660MZ43" ], "nt": "TTGTTCGTTTGGCAGGCTCTTTGGGGCGGGATAAGTTTTCTGCTTATTTTTCTGCTATTATATCGGCTAGCCAAACGGCGAGTGAAGGCTAAATACCACAATAATGAAGAAGGAATCGGAATGGGGGATGTATTGATCGCGCCTTATCTAGGAACCCTTCTTTTTGCAGGGATAGGAAATCGTGGAGATGTTTTACAGGGAGCAGGTATAATACTGATTTTTTTCGTCCTAAGTGGAAGTATTGGAATCTTTCGATTTATCCTTCAGAATAAAATCTTTAGAAAGAAAGCGAATTTTTTGAGTCCAAAAATGACCGAGCAGTCGGTTCCTTTTCTTCCCGCAATGATCTTAGCTGTTGTGTTCATTCTTCTCTTTCAAAACGCTTTATCTTCCCTTTTCTCATTCTAA", "aa": "MFVWQALWGGISFLLIFLLLYRLAKRRVKAKYHNNEEGIGMGDVLIAPYLGTLLFAGIGNRGDVLQGAGIILIFFVLSGSIGIFRFILQNKIFRKKANFLSPKMTEQSVPFLPAMILAVVFILLFQNALSSLFSF", "aa_hexdigest": "aca977da41d2c37ac9f6240b1239838c", "start_type": "TTG", "rbs_motif": "TTTA", "psc": { "uniref90_id": "UniRef90_A0A660MZ43", "query_cov": 0.8, "subject_cov": 1.0, "identity": 0.963, "score": 196.0, "evalue": 4.95e-63, "valid": true, "product": "Prepilin type IV endopeptidase peptidase domain-containing protein", "uniref50_id": "UniRef50_A0A660MZ43" }, "pscc": { "uniref50_id": "UniRef50_A0A660MZ43", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MZ43" ], "product": "Prepilin type IV endopeptidase peptidase domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_1712", "locus": "GCA003260355_01700" }, { "type": "cds", "sequence": "contig_2", "start": 47896, "stop": 48099, "strand": "-", "frame": 2, "gene": null, "product": "Prepilin peptidase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MNK2", "UniRef:UniRef90_A0A660MRK2" ], "nt": "ATGGCTTTTGGATTAATCATAGCAATCTTTCTTGGTCTGGTTTTTGGATCTTTTGGCTCAGTAATTCTTTCCAGACGAGGAGATAGTGAGAGTCTTGAGCAGGCTAGTTCGATTTTACGAGGAAGAAGTGAGTGTCCTCAGTGTAAAAAAAGATTGCAGCCCTGTGATCTTATCCCCGTTATCAGTTTCCTCCTCCAAAAATGA", "aa": "MAFGLIIAIFLGLVFGSFGSVILSRRGDSESLEQASSILRGRSECPQCKKRLQPCDLIPVISFLLQK", "aa_hexdigest": "8562540ee69bb0a251897f08a568ff97", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A660MRK2", "query_cov": 1.0, "subject_cov": 0.9436619718309859, "identity": 1.0, "score": 130.0, "evalue": 1.21e-38, "valid": true, "product": "Prepilin peptidase", "uniref50_id": "UniRef50_A0A660MNK2" }, "genes": [], "id": "NKOCPLAKPK_1713", "locus": "GCA003260355_01701" }, { "type": "cds", "sequence": "contig_2", "start": 48190, "stop": 48297, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTTTGGAAGAATTATATTATATGAGAAGAGATGAAATTAGGAAGCTTTTTGAGGGCTATAACTATTATGAGAATTATTCTTGTTTTTTTGGGGAAAATGTTTAG", "aa": "MALEELYYMRRDEIRKLFEGYNYYENYSCFFGENV", "aa_hexdigest": "a071e0eaec3f6bdcd9a306932b3d2cf7", "start_type": "ATG", "rbs_motif": "TTTTT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4456.938600000001, "isoelectric_point": 4.5459486007690435 }, "id": "NKOCPLAKPK_1714", "locus": "GCA003260355_01702" }, { "type": "cds", "sequence": "contig_2", "start": 48290, "stop": 48946, "strand": "+", "frame": 2, "gene": null, "product": "Peroxiredoxin", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2G6CCA4", "UniRef:UniRef90_A0A660MTB5" ], "nt": "ATGTTTAGACTCTTTATGTTTTTATATTCTTATAGCAATAGTAAAATGACTAAACTAGAACATTATTCTGCATGCGACTGCCACTGCAGTGCCTGCGAAGACAATTATTGTGAAGAATGCACGTGTTGCGACTGTGGAGAAAGCTATGACTCCAACAACTGTGCAAGGATCGAAAGGATCGTTCCAAACTTTAAACTTACGATGTATAATCCTCATAGCCAAGAGATTGAAAACAAAACTGTAAAGGATTACAGAAACAAATGGCTTGTTTTGTTTTTCTATCCAGCTGATTTTACCTTCGTCTGCCCAACAGAACTTAAAGATCTCAACCAAAAAGCTGAGGAAATCCGTGCAATGGGAGATGTTGAAGTCTTTGGTGTGAGTGTAGATTCTGTTTTCTCTCACAAGTCTTGGCTTGAATCCGAAGAAATGTTAAAAGGCTTCCAGTTTAATATGATCAGCGATAGAAATACTGTCCTCTCAAGGTACTTTGGTATTCTCAATACTGCAACTGGAAACGCAGAAAGAGGAACCTTTATCATCTCTCCAGACGGTGTTCTTAAGACGATAGAAATCCACACTGATGCTGTTGGAAGAAGTGCAAAAGAACTTGTAAGGAAGCTCTATGGATTAAAGTTTGTAACGGAAAACAAATGA", "aa": "MFRLFMFLYSYSNSKMTKLEHYSACDCHCSACEDNYCEECTCCDCGESYDSNNCARIERIVPNFKLTMYNPHSQEIENKTVKDYRNKWLVLFFYPADFTFVCPTELKDLNQKAEEIRAMGDVEVFGVSVDSVFSHKSWLESEEMLKGFQFNMISDRNTVLSRYFGILNTATGNAERGTFIISPDGVLKTIEIHTDAVGRSAKELVRKLYGLKFVTENK", "aa_hexdigest": "46d54f35cab42b3396615c5c02f3655d", "start_type": "ATG", "rbs_motif": "TTCTT", "psc": { "uniref90_id": "UniRef90_A0A660MTB5", "query_cov": 1.0, "subject_cov": 0.8449612403100775, "identity": 0.982, "score": 437.0, "evalue": 9.61e-155, "valid": true, "product": "Peroxiredoxin", "uniref50_id": "UniRef50_A0A2G6CCA4" }, "pscc": { "uniref50_id": "UniRef50_A0A2G6CCA4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2G6CCA4" ], "product": "Peroxiredoxin" }, "genes": [], "id": "NKOCPLAKPK_1715", "locus": "GCA003260355_01703" }, { "type": "cds", "sequence": "contig_2", "start": 49090, "stop": 49311, "strand": "+", "frame": 1, "gene": null, "product": "Cupin", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0E9MKJ9", "UniRef:UniRef90_A0A328ECS5" ], "nt": "ATGAAAGGATATCACGCAAATATCGAAGAGCTTACACTAAAAAATGACTATTTTAGGCAAGTACTTTATACTGCAAAGGGACTTCAACTTGTGTTGATGAGTCTTCTTCCTGGAGAAGAGATCGGATTAGAAGTTCATCATGATAATGTGCAGTTTTTCAGATTTGAAGCAGGGGAGGGGAAAGTAATCATTGATGATAATGAGTATTTGGTACAGGACTGA", "aa": "MKGYHANIEELTLKNDYFRQVLYTAKGLQLVLMSLLPGEEIGLEVHHDNVQFFRFEAGEGKVIIDDNEYLVQD", "aa_hexdigest": "563ba5a63ff284e65341540fe7b04876", "start_type": "ATG", "rbs_motif": "TTTTT", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 49090, "stop": 49500, "observations": { "mutation": [ 49309 ], "stop": [ 49309 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328ECS5", "query_cov": 1.0, "subject_cov": 0.5367647058823529, "identity": 1.0, "score": 150.0, "evalue": 2.62e-45, "gene_start": 1, "gene_end": 73, "reference_start": 1, "reference_end": 73, "reference_sequence": "MKGYHANIEELTLKNDYFRQVLYTAKGLQLVLMSLLPGEEIGLEVHHDNVQFFRFEAGEGKVIIDDNEYLVQDGSSIIVPAGAQHNVINTSSTEPLKMYTIYSPAHHKDGVVHTTKEIAEQAEHDGTDEFDGITTE" }, "paralog": false, "identity": 0.9852941176470589, "score": 272.0, "evalue": 7.91e-96, "description": "Internal stop codon at 49309. Nonsense mutation around 49309.", "psc": { "uniref90_id": "UniRef90_A0A328ECS5", "product": "Cupin", "uniref50_id": "UniRef50_A0A0E9MKJ9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328ECS5", "UniRef:UniRef50_A0A0E9MKJ9" ] }, "pscc": { "uniref50_id": "UniRef50_A0A0E9MKJ9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0E9MKJ9" ], "product": "Cupin type-2 domain-containing protein" } }, "id": "NKOCPLAKPK_1716", "locus": "GCA003260355_01704" }, { "type": "cds", "sequence": "contig_2", "start": 49381, "stop": 49500, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATACTATTTATTCTCCAGCGCACCACAAAGACGGCGTAGTTCACGCAACCAAGGAAATCGCTGAGCAAGCAGAACATGATGGAACCGATGAATTTGACGGTATAACTACCGAGTAA", "aa": "MYTIYSPAHHKDGVVHATKEIAEQAEHDGTDEFDGITTE", "aa_hexdigest": "28d1ac57b06ca88404c803fdf21325e1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4344.594399999998, "isoelectric_point": 4.671619606018066 }, "id": "NKOCPLAKPK_1717", "locus": "GCA003260355_01705" }, { "type": "cds", "sequence": "contig_2", "start": 49598, "stop": 50032, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTCAGCATTTTGACTATTGGGCACTAAAAAGAGTCAATCAAGGGGCTGCAGTACTCGAGCCAGGAGTCATTACTCAATCGAGTGATTTTGACCAAGATTCGCGATTTCCTACGGGAGCAGTGTTGGGGACAGAACAGCTCACTTGCAACCAAGGCAGTTCGGCAAGATATTATGATGAGCGACAGAGGAAAGTCAGCGGATTTGATACCTTTGGAAATACTATTTCTCAGACCTATCATCTCTATGGCTTTAGGAGGCCCCTACGAGCATATCCTCATTCCCTCAGCCGAAAACGGGAAGCTAAAGACCTCCTCTTAGATTTTTCTCTTCCAACGGGGAGTTATGCAAGTGTTTTTCTAGCTACACTTTTAGAAATGATCGATCCTAAAGGTTGCCTCAGCAACGGACTTTTGATTCCGCTCATTTCATAG", "aa": "MLQHFDYWALKRVNQGAAVLEPGVITQSSDFDQDSRFPTGAVLGTEQLTCNQGSSARYYDERQRKVSGFDTFGNTISQTYHLYGFRRPLRAYPHSLSRKREAKDLLLDFSLPTGSYASVFLATLLEMIDPKGCLSNGLLIPLIS", "aa_hexdigest": "970cb510a87def1b644cf9fed41cb0ca", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16156.100299999987, "isoelectric_point": 7.788763999938965 }, "id": "NKOCPLAKPK_1718", "locus": "GCA003260355_01706" }, { "type": "cds", "sequence": "contig_2", "start": 50531, "stop": 50758, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAGCGAATTAAAAAAAGACTGTATGTATTTTTCTTTCAAAACTCAGCCAGAGGATTTTATTGTAGAGGAGCTTTTACCTTTTCAGTTAAGCGGAAAGGGAGAGTTCCTTTATGTTTTCTTCGAGAAAAAACTAGTAAATACCATGGAGGTTATCCTCTCACTATGCCAGACGCTGGGGCTCAAAAGGGGGGATATTGGGATTGCAGGACTAAAAGATAAAGCCTGA", "aa": "MSELKKDCMYFSFKTQPEDFIVEELLPFQLSGKGEFLYVFFEKKLVNTMEVILSLCQTLGLKRGDIGIAGLKDKA", "aa_hexdigest": "343d5297c2116daca5631ab36dce99f3", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8571.029199999992, "isoelectric_point": 5.33436222076416 }, "id": "NKOCPLAKPK_1719", "locus": "GCA003260355_01707" }, { "type": "cds", "sequence": "contig_2", "start": 50755, "stop": 51057, "strand": "-", "frame": 2, "gene": null, "product": "Histidine phosphatase family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7W4HGU7", "UniRef:UniRef90_A0A328EGA0" ], "nt": "TTGAGTCGCCTTTTCATCAAGAGTGATACCGTAGAGCATAGTCAGACAATAGAAGAAACACGGGATCAGGTGCGTGAACGTTGCAAACTCTTTCTTCGCGAGCTTACCAAAACGTATCCAAGCTCTTCTCGTATCCTCATTGTGGGAAATCCTATCATAAATAGTGCTATCATAGCAACCTTGCTCAAAAAGGAAGGACAAGAAGAGCGTTTTGTTACATCTCCTTGTTCACTTAGCCGTTGTCGCTTTGTAGAGGATAGACGAGAAATGCTCTCTTTCAACCAACTTGGGCATTTATATTGA", "aa": "MSRLFIKSDTVEHSQTIEETRDQVRERCKLFLRELTKTYPSSSRILIVGNPIINSAIIATLLKKEGQEERFVTSPCSLSRCRFVEDRREMLSFNQLGHLY", "aa_hexdigest": "4f456ca6525cce97c61e7777e2058f2f", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 50746, "stop": 51357, "observations": { "mutation": [ 50757, 51135 ], "stop": [ 50757, 51135 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EGA0", "query_cov": 1.0, "subject_cov": 0.4694835680751174, "identity": 0.97, "score": 191.0, "evalue": 3.3e-60, "gene_start": 1, "gene_end": 100, "reference_start": 111, "reference_end": 210, "reference_sequence": "MELIITCNGEHVNTHCSTLGHCNDRLSDKGVLDSLRLAQALKKEKIDFIFCSDIGFRRTTTDLVFETLENDRFQIIVDPRIREKGAEICTKLTRETFENTTGNNPEQVKLLSRLFIKSDTVEHSQTIEETWDQVRERCKLFLRELTKTYPSSSRILIVGNPIINSAIIATLLKKEGQEERFVTSPCSLSRCRFVEDRREILSFNQLGHLYGAN" }, "paralog": false, "identity": 0.9753694581280788, "score": 392.0, "evalue": 1.08e-141, "description": "Internal stop codon at 50757, 51135. Nonsense mutation around 50757, 51135.", "psc": { "uniref90_id": "UniRef90_A0A328EGA0", "product": "Histidine phosphatase family protein", "uniref50_id": "UniRef50_A0A7W4HGU7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EGA0", "UniRef:UniRef50_A0A7W4HGU7" ] }, "pscc": { "uniref50_id": "UniRef50_A0A7W4HGU7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7W4HGU7" ], "product": "Histidine phosphatase family protein" } }, "id": "NKOCPLAKPK_1720", "locus": "GCA003260355_01708" }, { "type": "cds", "sequence": "contig_2", "start": 51133, "stop": 51387, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00300.27" ], "nt": "ATGGAACTCATCATCACTTGTAATGGAGAACACGTTAATACACATTGTTCTACCTTGGGACACTGCAATGATAGGCTTTCAGATAAAGGCGTACTAGATTCGCTGAGACTTGCACAAGCACTCAAAAAGGAAAAAATTGACTTTATCTTTTGTAGTGATATCGGATTTCGTAGGACAATTACGGATTTAGTCTTTGAGACTTTAGAAAATGATAGATTTCAGATCATCGTTGATCCAAGAATTAGAGAAAAATGA", "aa": "MELIITCNGEHVNTHCSTLGHCNDRLSDKGVLDSLRLAQALKKEKIDFIFCSDIGFRRTITDLVFETLENDRFQIIVDPRIREK", "aa_hexdigest": "b04c0ebc1b95e4ac551ad4cbfee8efff", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00300.27", "name": "His_Phos_1", "length": 67, "aa_cov": 0.7976190476190477, "hmm_cov": 0.9552238805970149, "evalue": 7.904739198540827e-06, "score": 22.101165771484375, "start": 17, "stop": 83 } ], "seq_stats": { "molecular_weight": 9707.052799999992, "isoelectric_point": 5.854950523376464 }, "id": "NKOCPLAKPK_1721", "locus": "GCA003260355_01709" }, { "type": "cds", "sequence": "contig_2", "start": 51467, "stop": 52057, "strand": "-", "frame": 1, "gene": null, "product": "Endolytic murein transglycosylase", "db_xrefs": [ "EC:4.2.2.29", "KEGG:K07082", "SO:0001217", "UniRef:UniRef50_A0A7W4HLC5", "UniRef:UniRef90_A0A328ECS9" ], "nt": "ATGAAGTCAGATTTTCCTTTTCTTTCAGCACTTCCTAGTGGAAAATCACTTGAAGGTTATCTCTATCCGGATACCTACTTCTTAGATCCTAATAGTTCCATACCAGAGCAACTTATTAAATCTCAGCTTAAAAACTTCAATCAAAAAGTCTGGATTCCTCTTGGAGATCAGCTCCAATCCTTTGGTTATGGATTTGATAGTTATCAACTTCTAACTCTTGCTTCTATAATAGAAAATGAGGAGAAGTTGGCAGGGAATAAGCCTATCATTGCCGGGATCTTTCTGAATAGACTGGAGAAGGGGATGAGATTGGATGCAGATGTAACTCTTTGTTATGGACTAAAGATTACCTACGACCAATGTAGAAATCAAATTCTTGCAAATCTCTCAGATGCAAGCAACCTCTACAATACAAGACAAAACTACGGGCTTCCTCCTACGCCAATTTCCTCACCGAGCGTAGAGACAATCTCAGCAACCTTAGACTTCAAAAAAACTGGAGCACTTTACTATCTCCATGATGCCAGTGGAAAGATTCACTACGGCACTACACTTGAAGAACATAATGAAAACAAAAAAAACTACCTATAA", "aa": "MKSDFPFLSALPSGKSLEGYLYPDTYFLDPNSSIPEQLIKSQLKNFNQKVWIPLGDQLQSFGYGFDSYQLLTLASIIENEEKLAGNKPIIAGIFLNRLEKGMRLDADVTLCYGLKITYDQCRNQILANLSDASNLYNTRQNYGLPPTPISSPSVETISATLDFKKTGALYYLHDASGKIHYGTTLEEHNENKKNYL", "aa_hexdigest": "3df70c66ec43fde14896dd8d80bbef71", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 51467, "stop": 52357, "observations": { "mutation": [ 52117, 52159, 52198 ], "stop": [ 52117, 52159, 52198 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328ECS9", "query_cov": 1.0, "subject_cov": 0.5664739884393064, "identity": 0.985, "score": 392.0, "evalue": 8.21e-136, "gene_start": 1, "gene_end": 196, "reference_start": 151, "reference_end": 346, "reference_sequence": "MRRPKKRKNRRIFMGGIIILFARLRILGRGAYNAPVYYPENTTVQIEKGDTINKFFPEQGFLKRIMLKFRLRNHRESIPMLQEGNYLLSGKYTKAELMNLIKEGPQRDVKRVTLLEGRSIYDIDAYLTELGQISAGQFIQKAEDQQFIQEMKSDFPFLSALPSGKSLEGYLYPDTYFLDPNSSIPEQLIKSQLKNFNQKVWIPLGDQLQSFGYGFDSYKLLTLASVIENEEKLAENKPIIAGIFLNRLEKGMRLDADVTLCYGLKITYDQCRNQILANLSDASNLYNTRQNYGLPPTPISSPSVETISATLDFKKTGALYYLHDASGKIHYGTTLEEHNENKKNYL" }, "paralog": false, "identity": 0.9763513513513513, "score": 577.0, "evalue": 3.32e-211, "description": "Internal stop codon at 52117, 52159, 52198. Nonsense mutation around 52117, 52159, 52198.", "psc": { "uniref90_id": "UniRef90_A0A328ECS9", "product": "Endolytic murein transglycosylase", "ec_ids": [ "4.2.2.29" ], "uniref50_id": "UniRef50_A0A7W4HLC5", "kegg_orthology_id": "K07082", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328ECS9", "EC:4.2.2.29", "UniRef:UniRef50_A0A7W4HLC5", "KEGG:K07082" ] }, "pscc": { "uniref50_id": "UniRef50_A0A7W4HLC5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7W4HLC5" ], "product": "Endolytic murein transglycosylase" } }, "id": "NKOCPLAKPK_1722", "locus": "GCA003260355_01710" }, { "type": "cds", "sequence": "contig_2", "start": 52691, "stop": 53074, "strand": "-", "frame": 1, "gene": "corA", "product": "Magnesium transport protein CorA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EGS3", "UniRef:UniRef90_A0A328EGS3" ], "nt": "ATGTATGATAAGATACTCCTTGGGCTCAATAAATTCAATCTTGATCTCATGGAAATGGAGAAACAAGTTTTTGATAAAAGTTTTGTTGATACAGATCTTCTCAAGAAGGTCCTTATCAAAAAGAGAAATACGGTATTGCTAAAAACTATTATTTCTCCACAAGAAGAAATTCTCGTAGAGCTCCAAAAAGTAACCGCAAATTTCTATGAAGGAGATTTAGATGTTTACTTTGAGGATCTTGAATACAAGACAAGTAAGATACTAGGGCATATTTCAATCATCCATGAAAATATTGATTCCATCGGAGAGATATTCAATACCCTTACAAATATGATGACCAACAAAAATATTGGGATATTGACGGTTTTGACGGTAATTATCTGA", "aa": "MYDKILLGLNKFNLDLMEMEKQVFDKSFVDTDLLKKVLIKKRNTVLLKTIISPQEEILVELQKVTANFYEGDLDVYFEDLEYKTSKILGHISIIHENIDSIGEIFNTLTNMMTNKNIGILTVLTVII", "aa_hexdigest": "368aaae0333c6c51da62e259ac8341e6", "start_type": "ATG", "rbs_motif": null, "genes": [ "corA" ], "truncated": "both", "pseudogene": { "start": 52544, "stop": 53374, "observations": { "mutation": [ 52651, 52693, 53137, 53263 ], "stop": [ 52651, 52693, 53137, 53263 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EGS3", "query_cov": 1.0, "subject_cov": 0.40189873417721517, "identity": 0.992, "score": 238.0, "evalue": 1.5e-76, "gene_start": 1, "gene_end": 127, "reference_start": 141, "reference_end": 267, "reference_sequence": "MPYKINRMLKNVLKIGKIKWIHLSKPTHHELEEAIKNLDLHEMVEQDLIEPSAHDKIDVYDDCIFLVIHFPKYNEKLGKYLSNEMNIILGRDFIITITKYPTNNIEKIRQEYEQEIKEEGEEAEEYKISPYYILYKIIDVMYDKILLGLNKFNLDLMEMEKQVFDKSFVDTDLLKNVLIKKRNTVLLKTIISPQEEILVELQKVTANFYEGDLDVYFEDLEYKTSKILGHISIIHENIDSIGEIFNTLTNMMTNKNIGILTVLTVIIGIMTMISGIYGMNIGLPFQNYPGAFIVLMGFMVIMAVFTVLIFKKKKRL" }, "paralog": false, "identity": 0.9492753623188406, "score": 509.0, "evalue": 9.38e-186, "description": "Internal stop codon at 52651, 52693, 53137, 53263. Nonsense mutation around 52651, 52693, 53137, 53263.", "psc": { "uniref90_id": "UniRef90_A0A328EGS3", "product": "Magnesium transport protein CorA", "uniref50_id": "UniRef50_A0A328EGS3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EGS3", "UniRef:UniRef50_A0A328EGS3" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EGS3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EGS3" ], "product": "Magnesium transport protein CorA" } }, "id": "NKOCPLAKPK_1723", "locus": "GCA003260355_01711" }, { "type": "cds", "sequence": "contig_2", "start": 53135, "stop": 53242, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATATCATTTTAGGGAGAGATTTTATTATTACGATCACCAAGTATCCGACCAATAATATCGAAAAGATTAGACAGGAATACGAACAAGAAATCAAAGAAGAGTGA", "aa": "MNIILGRDFIITITKYPTNNIEKIRQEYEQEIKEE", "aa_hexdigest": "cd30e7007630e330215e3efad92ba091", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4283.854, "isoelectric_point": 4.92949810028076 }, "id": "NKOCPLAKPK_1724", "locus": "GCA003260355_01712" }, { "type": "cds", "sequence": "contig_2", "start": 53688, "stop": 53966, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGGAAAACTCTTCTTTAGTCTCTTGGCCATTGCCGGGCTTATCGGTGTATCTTTTGCACAGGATAGCGGTGCCAAGGTTGGACTCTCAATGACTAATCTCCCTGCTTCATTTTCTGGAGAATTTACTCTTGAACTTACGACGACAGCAGGAGCTCTTTCTGGAGCAGAATCTTTTAGTCTCTCTTTTTCTGAGTGTGGAAGTGGACAGACTGAACTCTGGTATGGAAACCAGAAAAAATCTGATCAGATTTCATCTTTTTCTCTCGCTTCATGA", "aa": "MKGKLFFSLLAIAGLIGVSFAQDSGAKVGLSMTNLPASFSGEFTLELTTTAGALSGAESFSLSFSECGSGQTELWYGNQKKSDQISSFSLAS", "aa_hexdigest": "4d785a115c348b690bcbb8a976658edf", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9514.596099999993, "isoelectric_point": 4.766768074035644 }, "id": "NKOCPLAKPK_1725", "locus": "GCA003260355_01713" }, { "type": "cds", "sequence": "contig_2", "start": 54210, "stop": 54719, "strand": "+", "frame": 3, "gene": null, "product": "S-layer-like y domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MSN6", "UniRef:UniRef90_A0A660MSN6" ], "nt": "TTGGATAAAGAACTTGAAAATGCTCTTGATTGGGCTGTTCGTCAAGGGATCATTTCTCAGGCAGATAAAGCTCGCTTCAATAATCCAATGACAAGAATGGAACTTGCACAGATGATTCTTCCTGTAGGTGACAAACTTGGACGCGCAGCTAACGAAGGGAAAGCATGTAGCTTTAAAGATCTTGTTGGAACCCCAGAAGCCAGCATTGCTACAGCAAAAAGAGTTTGCCAACTCAATGTAATGGGAATCTATCCAAATGAAGAAGTACTTGAGAACTTTATGCCAGATCAAATTGTAGACAGAGCTCAAATGTTGACGACGATCTCTAGGATGCTCTGGGGAAATAGCTATAACCAAGGAGGAAAGACCTTCTATGAAAAGCACTTTGACAAAGTAAAGGAAGAAAATGTTGTCAGAAAGCCATTTGCCAATCTTGTGGAACTTAGAAGTTATTTCTATCTTGTACTCCAGAGAGCAGAAGAAAAGGGACTTTTAAAATGGCAAAAGTAG", "aa": "MDKELENALDWAVRQGIISQADKARFNNPMTRMELAQMILPVGDKLGRAANEGKACSFKDLVGTPEASIATAKRVCQLNVMGIYPNEEVLENFMPDQIVDRAQMLTTISRMLWGNSYNQGGKTFYEKHFDKVKEENVVRKPFANLVELRSYFYLVLQRAEEKGLLKWQK", "aa_hexdigest": "d91bec69269082a9bb26722aeba38509", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 53910, "stop": 54719, "observations": { "mutation": [ 53964, 54165 ], "stop": [ 53964, 54165 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A660MSN6", "query_cov": 1.0, "subject_cov": 0.49271137026239065, "identity": 0.976, "score": 338.0, "evalue": 4.79e-115, "gene_start": 1, "gene_end": 169, "reference_start": 175, "reference_end": 343, "reference_sequence": "MKGKLFFSLLAIAGLIGVSFAQDSGAKVGLSMTNLPASFSGEFTLELTTTAGALSGAESFSLSFSECGSGQTELWYGNQKKSDQISSFSLASGEKKSFPLKIKASNIHGCQLNFKLTDTAGVKVAETSMVLNSNCDLPIDFDQEYVLSQQANNSQPGKAGVNKTTSVNNTTRGSLDKELENALDWAVRQGIISQADKARFNNPMTRMELAQMILPVGDKLGRAANEGKACSFRDLVGTPEASIATAKRVCQLNVMGIYPNEEALENFMPDQIVDRAQMLTTISRMLWGNSYNQGGKTFYEKHFDKVKEENVVRKPFAHLVELRSYFYLVLQRAEEKGLLKWQK" }, "paralog": false, "identity": 0.966542750929368, "score": 520.0, "evalue": 7.05e-189, "description": "Internal stop codon at 53964, 54165. Nonsense mutation around 53964, 54165.", "psc": { "uniref90_id": "UniRef90_A0A660MSN6", "product": "S-layer homology domain-containing protein", "uniref50_id": "UniRef50_A0A660MSN6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A660MSN6", "UniRef:UniRef50_A0A660MSN6" ] }, "pscc": { "uniref50_id": "UniRef50_A0A660MSN6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MSN6" ], "product": "S-layer homology domain-containing protein" } }, "id": "NKOCPLAKPK_1726", "locus": "GCA003260355_01714" }, { "type": "cds", "sequence": "contig_2", "start": 54909, "stop": 55052, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAATTTACATCAAAACTCCACTGCTAAATAATTCACAAACAGTTCCCCATATGGTAGAGCACTGCATCAGAAAGAATAAAAAGCCTGATGTAAGGCGGTTCTTTGAGTCACATCTTCCTTGTGAGCATTGGATTAATTGA", "aa": "MKIYIKTPLLNNSQTVPHMVEHCIRKNKKPDVRRFFESHLPCEHWIN", "aa_hexdigest": "c847932ec13b31fbc4deceb587c6630f", "start_type": "ATG", "rbs_motif": "TTATT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5729.687999999998, "isoelectric_point": 9.509940910339356 }, "id": "NKOCPLAKPK_1727", "locus": "GCA003260355_01715" }, { "type": "cds", "sequence": "contig_2", "start": 55209, "stop": 55373, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGCAGCAACTTATCAGTCCAGAGATTCGCCTAAACACCTGGAAGGGCAAAACCTGGAAAAAGGTCAATCAATACCACAAAACCTATTATCAAGATCAGAATTTTCTGATTTTTGAGGATCAAGTATCTGCTCATCAAGAGTATGAGTTGCAATTTATATGA", "aa": "MLQQLISPEIRLNTWKGKTWKKVNQYHKTYYQDQNFLIFEDQVSAHQEYELQFI", "aa_hexdigest": "e5e6a1c4202146ad0dddaadc9341cdcc", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6720.5344000000005, "isoelectric_point": 6.707705879211425 }, "id": "NKOCPLAKPK_1728", "locus": "GCA003260355_01716" }, { "type": "cds", "sequence": "contig_2", "start": 56013, "stop": 56312, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00730.31" ], "nt": "TTGCATGGGAGGAAGCTTCCGTGGAGGGCGACCCAAGATCCCTATGCTATTCATATTTCTGAGGTAATGAGTCAGCAGACACAAGTAGACCGTGTCATTCCTTATCGAAAGCAATGGATAGCTGAGATTCCTGATTATAAAAGTTTGGCTCAGCTCCCTAAACCTGAATTGCTAAGCCATCGATCAGGGCTTGGTTTTAATAGTAGAGCATTAAGGCTTCAACAGTGTGCACTGGAAGTCGTCGATCGTTATCAGGGGAACCTTCCGCAGAGCAAAGATCAGCTTTTATTACTTCCTTGA", "aa": "MHGRKLPWRATQDPYAIHISEVMSQQTQVDRVIPYRKQWIAEIPDYKSLAQLPKPELLSHRSGLGFNSRALRLQQCALEVVDRYQGNLPQSKDQLLLLP", "aa_hexdigest": "7f1f9cb30ea782e30b899f8f968162f4", "start_type": "TTG", "rbs_motif": "TTCTT", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00730.31", "name": "HhH-GPD", "length": 66, "aa_cov": 0.6363636363636364, "hmm_cov": 1.0, "evalue": 3.942796616918306e-12, "score": 42.94121170043945, "start": 19, "stop": 81 } ], "seq_stats": { "molecular_weight": 11476.113499999987, "isoelectric_point": 9.5132287979126 }, "id": "NKOCPLAKPK_1729", "locus": "GCA003260355_01717" }, { "type": "cds", "sequence": "contig_2", "start": 56370, "stop": 56804, "strand": "+", "frame": 3, "gene": null, "product": "Adenine DNA glycosylase", "db_xrefs": [ "EC:3.2.2.31", "KEGG:K03575", "SO:0001217", "UniRef:UniRef50_A0A288DFT0", "UniRef:UniRef50_A0A3D5YFP1", "UniRef:UniRef90_A0A660MRJ2" ], "nt": "GTGATTGACACAAACATTAGGAGGGTGCTTATTTTTCTTTTGAAGCTGGAGGAAAGTATTTCTCTTCCTGCCTTGGAGCAACTTGCTAAAGAACTTATCCCTGAAGGAAGAAGCAGAGATCGGCACAATGCACTAATGGACTACGGAGCAACCCATCTCACTGCGAAGAAGACGAAAATTAAATCGCTAGGCAAACAAAGCAAATTTGAGGGGTCAGATAGAGAAGTAAGAGGACGAATCCTGAAACAACTCACTGGAAATACTTCCGTTCTAGGGAAGCAGTATAGTAGCTGGGATGAAAAAAAGCAAAGGAGAGTCCTTTTATTGTCCGATATTGTGCAGGAATTTCCTCATAAAGAGATACCGAAAATCCTCCAAACTATGGAAGCCGATCATCTCATTAGATATCAAGATCATGAAATCTGGATTGAATAA", "aa": "MIDTNIRRVLIFLLKLEESISLPALEQLAKELIPEGRSRDRHNALMDYGATHLTAKKTKIKSLGKQSKFEGSDREVRGRILKQLTGNTSVLGKQYSSWDEKKQRRVLLLSDIVQEFPHKEIPKILQTMEADHLIRYQDHEIWIE", "aa_hexdigest": "f37e48eaf161ae8ae2f5f26386d77843", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3D5YFP1", "query_cov": 0.9236111111111112, "subject_cov": 1.0725806451612903, "identity": 0.5710000000000001, "score": 131.0, "evalue": 8.6e-37, "valid": false, "uniref50_id": "UniRef50_A0A3D5YFP1" }, "pscc": { "uniref50_id": "UniRef50_A0A3D5YFP1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3D5YFP1" ] }, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 56070, "stop": 56804, "observations": { "mutation": [ 56310, 56316 ], "stop": [ 56310, 56316 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A660MRJ2", "query_cov": 0.9930555555555556, "subject_cov": 0.4847457627118644, "identity": 0.965, "score": 271.0, "evalue": 1.38e-89, "gene_start": 2, "gene_end": 144, "reference_start": 153, "reference_end": 295, "reference_sequence": "MSQIFGSFFILRKAMKPDQIATLQREILGRYALHGRKLPWRATQDPYAIHISEVMSQQTQVDRVIPYRKQWIAEIPDYKSLAQLPKPELLSHRSGLGFNSRALRLQQCAQEVVDRYHGNLPQSKDQLLLLPGIGPYTASAICAFAWNLPEPVIDTNIRRVLIFLLKLDESISLPALEQVAKELIPEGRSRDRHNALMDYGATHLTAKKTKIKSLGKQSKFEGSDREVRGRILKQLTGNATVLGKQYSSWDEKRQRRVLLLSDIVQEFPHKEIPKILQTMEADHLIRYQDHEIWIE" }, "paralog": false, "identity": 0.9631147540983607, "score": 468.0, "evalue": 1.65e-169, "description": "Internal stop codon at 56310, 56316. Nonsense mutation around 56310, 56316.", "psc": { "uniref90_id": "UniRef90_A0A660MRJ2", "product": "Adenine DNA glycosylase", "ec_ids": [ "3.2.2.31" ], "uniref50_id": "UniRef50_A0A288DFT0", "kegg_orthology_id": "K03575", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A660MRJ2", "EC:3.2.2.31", "UniRef:UniRef50_A0A288DFT0", "KEGG:K03575" ] }, "pscc": { "uniref50_id": "UniRef50_A0A288DFT0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A288DFT0" ], "product": "Adenine DNA glycosylase" } }, "id": "NKOCPLAKPK_1730", "locus": "GCA003260355_01718" }, { "type": "cds", "sequence": "contig_2", "start": 57186, "stop": 57704, "strand": "-", "frame": 3, "gene": null, "product": "Polysaccharide pyruvyl transferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2N8NGC4", "UniRef:UniRef90_A0A2N8NGC4" ], "nt": "GTGATCGAGGAAACCAGACCCTTCCCTCACAATGGACGAAATATCCCATTCCTCTACTTGAGATCCGTCCTGCAGAGACGCTTTGAACTATGGGGCGGTTTTGGACCCCAGACGAAATGGACAACGAAACTCCTTTCTTCCTTTCTCTTCCACTATGCAAAAAGGGTTCTAGCAAGAGATGAACATTCCTATCACTTGGCAAAAAAAGCAAACCCAAACTCCCACAAAAGAGCAGACTTTAGTAAGGGAATTTTAGACTACTTTCTCGAGCATGCTCCTGCTTATCCAGAATCTGCTCCTTACGGACTCCTAAATTACTCCCCCTTGACGAGCACGGCACCCGACCGCCTCAAAAATCAGCTTGCTTCGACATTTAAGCGACGATATTTCGCCTCAGACGCTAAATTTGATGCTCCTTTTTTCCATTCCCTGAGCAAAATTTTGCCACTTACGGCATATGATTGGACAACGAAGTCTCTTCCAGAGACACTCTCCTTTATCAGAAAAAGTAAAGTCTGA", "aa": "MIEETRPFPHNGRNIPFLYLRSVLQRRFELWGGFGPQTKWTTKLLSSFLFHYAKRVLARDEHSYHLAKKANPNSHKRADFSKGILDYFLEHAPAYPESAPYGLLNYSPLTSTAPDRLKNQLASTFKRRYFASDAKFDAPFFHSLSKILPLTAYDWTTKSLPETLSFIRKSKV", "aa_hexdigest": "52e0e40f2813de50ebc9f7fa618daac8", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 57090, "stop": 58004, "observations": { "mutation": [ 57182, 57188, 57716 ], "stop": [ 57182, 57188, 57716 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A2N8NGC4", "query_cov": 1.0, "subject_cov": 0.5276073619631901, "identity": 0.9940000000000001, "score": 355.0, "evalue": 4.39e-122, "gene_start": 1, "gene_end": 172, "reference_start": 123, "reference_end": 294, "reference_sequence": "MQIYLKGYYGYQNFGDEMLLFGVIEKIFSDYQVEQLVIEVGDLEWIKQRKERNLNFITEYYRFRGVEVNREKIQFFQPHQRSQTILPQRIKALLPAKWLTYLALILGKSPYRSFFKVFGGGEVIEETRPFPHNGRNIPFLYLRSVLQRRFELWGGFGPQTKWTTKLLSSFLFHYAKRVLARDEHSYHLAKKANPNSHKRADFSKGILDYFLEHAPAYPESAPYGLLNYSPLTSTAPDRLKNQLASTFKRRYFASDAKFDAPFFHSLSKILPLTAYDWTTKSLPETLSFIRKSKVGIGSRLHFLYCFKVFELPFHTLHQSQKLQHNL" }, "paralog": false, "identity": 0.9901315789473685, "score": 613.0, "evalue": 2.98e-226, "description": "Internal stop codon at 57182, 57188, 57716. Nonsense mutation around 57182, 57188, 57716.", "psc": { "uniref90_id": "UniRef90_A0A2N8NGC4", "product": "Polysaccharide pyruvyl transferase", "uniref50_id": "UniRef50_A0A2N8NGC4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A2N8NGC4", "UniRef:UniRef50_A0A2N8NGC4" ] }, "pscc": { "uniref50_id": "UniRef50_A0A2N8NGC4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2N8NGC4" ] } }, "id": "NKOCPLAKPK_1731", "locus": "GCA003260355_01719" }, { "type": "cds", "sequence": "contig_2", "start": 57714, "stop": 58019, "strand": "-", "frame": 3, "gene": null, "product": "Polysaccharide pyruvyl transferase", "db_xrefs": [ "SO:0001217", "UniParc:UPI000CACA2EC", "UniRef:UniRef100_A0A2N8NGC4", "UniRef:UniRef50_A0A2N8NGC4", "UniRef:UniRef90_A0A2N8NGC4" ], "nt": "ATGCTGCTTTTTGGGGTAATTGAAAAGATCTTTTCTGATTATCAGGTGGAGCAACTCGTAATAGAGGTAGGAGATTTAGAATGGATCAAGCAACGAAAAGAAAGAAATCTGAATTTCATCACGGAGTATTATCGCTTTCGTGGAGTCGAGGTCAACCGAGAAAAGATTCAGTTTTTTCAGCCTCATCAACGAAGTCAAACCATCCTCCCCCAGCGAATTAAAGCACTTCTCCCTGCGAAATGGCTAACCTATTTAGCATTGATCCTCGGCAAAAGCCCCTACCGCTCGTTTTTCAAAGTCTTTTGA", "aa": "MLLFGVIEKIFSDYQVEQLVIEVGDLEWIKQRKERNLNFITEYYRFRGVEVNREKIQFFQPHQRSQTILPQRIKALLPAKWLTYLALILGKSPYRSFFKVF", "aa_hexdigest": "0a5cfaafc04bf982c4f58ce02ecddecb", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000CACA2EC", "uniref100_id": "UniRef100_A0A2N8NGC4", "db_xrefs": [ "SO:0001217", "UniParc:UPI000CACA2EC", "UniRef:UniRef100_A0A2N8NGC4" ] }, "ips": { "uniref100_id": "UniRef100_A0A2N8NGC4", "uniref90_id": "UniRef90_A0A2N8NGC4" }, "psc": { "uniref90_id": "UniRef90_A0A2N8NGC4", "product": "Polysaccharide pyruvyl transferase", "uniref50_id": "UniRef50_A0A2N8NGC4" }, "pscc": { "uniref50_id": "UniRef50_A0A2N8NGC4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2N8NGC4" ] }, "genes": [], "id": "NKOCPLAKPK_1732", "locus": "GCA003260355_01720" }, { "type": "cds", "sequence": "contig_2", "start": 58452, "stop": 58859, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00763.28" ], "nt": "GTGTATGTCAAATACAAACAGCTTTACGGAGAAAAGATTGGATTGCCGGCATTGGTGTTTGGGCAAGGGAAGGGGACTTCTTCAGATCCAGAGTTGACAGCTCTTCTTCAGCAAACACAACGAGACAAAAAACTGATCTTAGCCCTCATTCATCGGCTTAATCGTGAAGAAAGATGTGTGGGTATTATTATTCAGCTTCCTTTGCCTCCAGAATTTCAGCCCTACAAAAACGAATTGCTTTCGGCGATCACCCCAGCAAAAGACCTCGACGGTCTCAATGGCACGCTCATCGGTAAAAATTTCCTTGAGATGATCGATTTTTTGCCAGCGACTCCCAAGGCAGTAATCAGCCTTCTTGATTATTACAAGCTTGGAAACCTCCGCGGAAAGAGAGTAGCCGTGCTTTGA", "aa": "MYVKYKQLYGEKIGLPALVFGQGKGTSSDPELTALLQQTQRDKKLILALIHRLNREERCVGIIIQLPLPPEFQPYKNELLSAITPAKDLDGLNGTLIGKNFLEMIDFLPATPKAVISLLDYYKLGNLRGKRVAVL", "aa_hexdigest": "2b88c1d815d3b3dd6a1f261ea23800ef", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00763.28", "name": "THF_DHG_CYH", "length": 49, "aa_cov": 0.362962962962963, "hmm_cov": 0.9591836734693877, "evalue": 1.4939237382128797e-10, "score": 37.838191986083984, "start": 42, "stop": 90 } ], "seq_stats": { "molecular_weight": 15092.646799999979, "isoelectric_point": 9.351155281066895 }, "id": "NKOCPLAKPK_1733", "locus": "GCA003260355_01721" }, { "type": "cds", "sequence": "contig_2", "start": 58914, "stop": 59132, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGTTATATCAAGAGAAGTTTTTTTTAATCAATTCTTTTCTCTTTTTTCCCCCATCGCTCCATAAAAACGGGACTACGCTTGCTTTTGATAAGAATATTCGTAAGGTAGGAGTGAAAATTTATTCCTTACGATCAAGAACTATGCTGCTCTATGGAAAACCTCTTGCAGAAAAGATGGAAGCTGACCTGAGACAACAGGTGCATTCCACTTTTTGA", "aa": "MLLYQEKFFLINSFLFFPPSLHKNGTTLAFDKNIRKVGVKIYSLRSRTMLLYGKPLAEKMEADLRQQVHSTF", "aa_hexdigest": "b257292865a893715b9b894ec3f43b6a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8468.9139, "isoelectric_point": 9.993453788757325 }, "id": "NKOCPLAKPK_1734", "locus": "GCA003260355_01722" }, { "type": "cds", "sequence": "contig_2", "start": 59337, "stop": 60278, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF05257.22" ], "nt": "ATGAGAAATAATATTCTATCATTAGTAGTATTAGTCTTGTTAATAGGACTGATATCGTGTGAAAAAAATGATGATATAGAACTTGATGGCAAGACAACATCTCCTAGCCAGGAGGTAGTTGTTGGGTCTAGTCTTGAATTAAGATCCTTACAATGGCATGACTTGCCATATGTAGAATTTATTGGTAGATTGTACAGAACGAGAGGATGTCAAAAACTTTCTGACAACCGTTTCAAGTTTGTTGTTGAGCTTCTTCCGTATGGAACTAGTAATTTCAGTCAGCAAGAACTTTATGTAAAATTTTATCACAATTCTACAGGGACGCAGTTCTACCAAAAGATGAGAAACGTTTCTCCTCCGCATCAACTTCCCAAGTTTGAATTGGAGCAGTCATTTCTTAAGACTGGAGACTATTCTTACCGATATTTGGTACGAGAGAATGCGTTTACTAATGATCTAAGACAGTTGACCGCTAAAGGATCTACATTTTTTTCTTTGACAATACCTTCTGGACTTGATGATTATCACGCCAAAGCACGAAAAATATATGCAGGAGCTGCGTATGGTTATATGGATCAATGGAATTTTGGTTATGAAGAATGTGTTAGTTGGGTAGCATTGAAAGTCAATCAAATGTGGGGAACTAACACGGAATTCAATAACAGAATGTTTGGTGCTCCGATAGGTAACGCCAAATATTGGAAGAAAAGATTTCAGGAGAAGGGCTATTCTGTAAATAGATATCCGCGTGCGGGTGACATTATTTGGTGGAGCCGTGGTGGATATGGTGCAGGGCATGTAGGATTTGTTCACGAAGTTACAAACGGGACAATTCGCTATACTGAATACAATGGAAGTATTCCTCATGGATATTCGTATAGAGAGTTATCGACCTCACAGCTTGGTGATGCAGAATTTATTCACATTCAACGCAAGCTATAA", "aa": "MRNNILSLVVLVLLIGLISCEKNDDIELDGKTTSPSQEVVVGSSLELRSLQWHDLPYVEFIGRLYRTRGCQKLSDNRFKFVVELLPYGTSNFSQQELYVKFYHNSTGTQFYQKMRNVSPPHQLPKFELEQSFLKTGDYSYRYLVRENAFTNDLRQLTAKGSTFFSLTIPSGLDDYHAKARKIYAGAAYGYMDQWNFGYEECVSWVALKVNQMWGTNTEFNNRMFGAPIGNAKYWKKRFQEKGYSVNRYPRAGDIIWWSRGGYGAGHVGFVHEVTNGTIRYTEYNGSIPHGYSYRELSTSQLGDAEFIHIQRKL", "aa_hexdigest": "56ac18c4afcd847b4382cd7760813e1f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF05257.22", "name": "CHAP", "length": 94, "aa_cov": 0.2939297124600639, "hmm_cov": 0.8404255319148937, "evalue": 7.860592397379334e-16, "score": 54.84406661987305, "start": 193, "stop": 284 } ], "seq_stats": { "molecular_weight": 36183.3952, "isoelectric_point": 8.994967460632324 }, "id": "NKOCPLAKPK_1735", "locus": "GCA003260355_01723" }, { "type": "cds", "sequence": "contig_2", "start": 60641, "stop": 60787, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATTTTTTGGAGAAGTATGCTATTAAGGAGAAGGGACTCCTTTCTCTCGTTGTAGATGATAAAGATGTAGATAAAGAGGCTCAATTCTTTTATAGATGTGAAGAGCCCACTCTATGTGAGCAACTTAGACGAACTTCTCGATGA", "aa": "MDFLEKYAIKEKGLLSLVVDDKDVDKEAQFFYRCEEPTLCEQLRRTSR", "aa_hexdigest": "6bf9606a96ce200f2532354505bf9566", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5757.529499999998, "isoelectric_point": 4.957974433898924 }, "id": "NKOCPLAKPK_1736", "locus": "GCA003260355_01724" }, { "type": "cds", "sequence": "contig_2", "start": 60884, "stop": 61102, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTGACAAATAGCGCTAAGGATTCCTCGGCTACGGGAGTTGTAGAAGACAGCTTACCCCATTGTAAACCTTTAGACTTAAAGGATACTGAAGTTCTTTTGCTGGAAGACCAAGTAGTTAATTGCCATTATGAAGAAGGACACCTCAGGTATCTTACAATTGGTAGAGGATCTAGACCTTGGAAGGCGTTTGAACTCTATAAGGATTATATCAATTGA", "aa": "MLTNSAKDSSATGVVEDSLPHCKPLDLKDTEVLLLEDQVVNCHYEEGHLRYLTIGRGSRPWKAFELYKDYIN", "aa_hexdigest": "1667a8164dbab39a0b66d9b4e53816c1", "start_type": "ATG", "rbs_motif": "TTCTT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8224.188299999998, "isoelectric_point": 5.167085075378416 }, "id": "NKOCPLAKPK_1737", "locus": "GCA003260355_01725" }, { "type": "cds", "sequence": "contig_2", "start": 61340, "stop": 61747, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EGB2", "UniRef:UniRef90_A0A328EGB2" ], "nt": "TTGGGTCGCAGGGGACTCGCTGAGGGTCGAGAAACCACCCCGCTGAGCAGATTGGAGGTTTCCGAGGGTCGAGAAAGCACCCCGCTGAGCAGATTGGAGGTCGCTGAGGGTTCAGAAACCTTCCCGCTGAGTAGATTGGAGGTCGCCGAGGGTTCAGAAAGCTCACCGCTGAGCAGATTGGAGGTCGCCGAGGGTTCAGAAAGCTCACCGCTGAGCAGATTGGAGGTCGCCGAGGGTTCAGAAACCACCCCGCTGAGCAGATTGGAGGTCGCTGAGGGCTCAGAAACCACCCCGCTGAGCAGATTGGAGGTCGCTGAGGATCGAGAAAGCTTCCCGCAATACAGGCAAACGGGGGAATCTTTAGAGATGGTAAGGTATTTTATGCTTTTTCATAGTAGGATGTGCTAG", "aa": "MGRRGLAEGRETTPLSRLEVSEGRESTPLSRLEVAEGSETFPLSRLEVAEGSESSPLSRLEVAEGSESSPLSRLEVAEGSETTPLSRLEVAEGSETTPLSRLEVAEDRESFPQYRQTGESLEMVRYFMLFHSRMC", "aa_hexdigest": "9df280a84db4ef5543c7c094ee828a26", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A328EGB2", "query_cov": 1.0, "subject_cov": 0.9, "identity": 0.993, "score": 57.4, "evalue": 8.61e-08, "valid": true, "uniref50_id": "UniRef50_A0A328EGB2" }, "pscc": { "uniref50_id": "UniRef50_A0A328EGB2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EGB2" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14940.369899999992, "isoelectric_point": 4.6428022384643555 }, "id": "NKOCPLAKPK_1738", "locus": "GCA003260355_01726" }, { "type": "cds", "sequence": "contig_2", "start": 62032, "stop": 62358, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAACTTCTTATCTCAGCAAGACCTACAGAACCTATGCTTTTTAATGGAATGGACTATATTAAGGCTGAGATTAGCAAATTAGCTCCTAGCGCCTCCGCTGATGAATGTCAGCAAAAGGTAGCAGAAGCAGCACAGTTTTATCTCAACCTCATCAATGAAACTGCCGAAAGAGCGGTTTTGAATGGGTTGGGAATTATTAATGATGGGGACAATGTCCTTACCCACTGTCACAGTTCCTCTGCCGTGAAAACATTGAAGCTCCATAAAGTAAAAGGTTTGAAGTTTAAAGTGTACAACACTGAGACAAGACCGCTTTTTCAATGA", "aa": "MELLISARPTEPMLFNGMDYIKAEISKLAPSASADECQQKVAEAAQFYLNLINETAERAVLNGLGIINDGDNVLTHCHSSSAVKTLKLHKVKGLKFKVYNTETRPLFQ", "aa_hexdigest": "d735bd38b9ef2ade8e96d2ba066a828a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11950.645299999986, "isoelectric_point": 6.8208723068237305 }, "id": "NKOCPLAKPK_1739", "locus": "GCA003260355_01727" }, { "type": "cds", "sequence": "contig_2", "start": 62434, "stop": 62808, "strand": "+", "frame": 1, "gene": null, "product": "Initiation factor 2B", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A351Y5K4" ], "nt": "ATGGATGAAGAAAGTGGAACAGATTTGATGATGGATTGTGTGATTATTGGTTGCGATGCGATCAAGCTTGATGGAGGAGTGATCAATAAGGTAGGGAGTTATAGCGTTGGGCTTTCTGCGCTGTTTGCCAATGTTCCGGTGTATATCGCCGGGAATCTCCTCAAGGTCGATATTCATGACAAAATTCATATCGAGAAGAGGCAGGATCATGAGGTTTGGGAGGATTCTCCAGAAGGATTACAGTTTTTAAATCTGGCTTTTGATAAAATTCCGCCAAAGTTCATCAGGGGGATTATTACAGAATTTGGGATCATCAAACCAAGGGATATTAGAGCAGTCGTGGAAAGAGAGTATCCATGGATGAAGATAGCATAG", "aa": "MDEESGTDLMMDCVIIGCDAIKLDGGVINKVGSYSVGLSALFANVPVYIAGNLLKVDIHDKIHIEKRQDHEVWEDSPEGLQFLNLAFDKIPPKFIRGIITEFGIIKPRDIRAVVEREYPWMKIA", "aa_hexdigest": "96ebc0e61ce83fe21835180b6969e6d8", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A351Y5K4", "query_cov": 0.9274193548387096, "subject_cov": 0.8156028368794326, "identity": 0.5479999999999999, "score": 139.0, "evalue": 4.38e-40, "valid": false, "product": "Initiation factor 2B", "uniref50_id": "UniRef50_A0A351Y5K4" }, "pscc": { "uniref50_id": "UniRef50_A0A351Y5K4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A351Y5K4" ], "product": "Initiation factor 2B" }, "genes": [], "id": "NKOCPLAKPK_1740", "locus": "GCA003260355_01728" }, { "type": "cds", "sequence": "contig_2", "start": 62874, "stop": 63284, "strand": "-", "frame": 3, "gene": "secF", "product": "Protein translocase subunit SecF", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MWN9", "UniRef:UniRef90_A0A660MWN9" ], "nt": "GTGCTGAGTATTAGTATTCCAGCGGGTGCGTATGGAATCTGGATGGCACTAGATCCAACAACACAGATAGATACGGTGTTCATCATCGCAATACTCACAATCATTGGATATGGTATCAATGATGTGATCATTATCTTCGATAGGATCAGGGAAAATATGATTCGTTCAAATGAAAAGATCGCTTACGCAAATGTCTTTGATGAAAGTATCCGACAAACCTTCAAGAGATCCTTAGGAACATCTATTTCTACATTGTTAGTGCTTATTGCAATGTTTGTCTTCGGAACGGGCGTGATCCAGAGATTCTCATTCACCGTAGGAGTGGGTGTGGTAGTATCCTTCCTGAGTAGTATCTTTGTGGCAGCTTCAACGGCCTACCTTCTTATCAATCTTACCAAAAAAGTTCGCTAA", "aa": "MLSISIPAGAYGIWMALDPTTQIDTVFIIAILTIIGYGINDVIIIFDRIRENMIRSNEKIAYANVFDESIRQTFKRSLGTSISTLLVLIAMFVFGTGVIQRFSFTVGVGVVVSFLSSIFVAASTAYLLINLTKKVR", "aa_hexdigest": "e4069cdbcbefaa3e3297f74ec1be5d83", "start_type": "GTG", "rbs_motif": null, "genes": [ "secF" ], "truncated": "3-prime", "pseudogene": { "start": 62874, "stop": 63584, "observations": { "mutation": [ 63344, 63383 ], "stop": [ 63344, 63383 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A660MWN9", "query_cov": 1.0, "subject_cov": 0.4121212121212121, "identity": 0.993, "score": 245.0, "evalue": 3.09e-79, "gene_start": 1, "gene_end": 136, "reference_start": 195, "reference_end": 330, "reference_sequence": "MEGKNYSLGRFYFIFLMMRKYFSIVKRAYLWILIAITATVVSWVLFIFSAQFSEEFTGGISVVFNGKVKDAEFKEQLAQGLAEKGFPNLKVHLDNNVGQTKIKINNRLKDNEEVNKLSLAISNYLVESQTISSESDIVEQVVIGPSVGKYMTNTASRALGLGLLAMMIYMIFSFGTIRKNIAPGILATVVLFTSVLSISIPAGAYGIWMALDPTTQIDTVFIIAILTIIGYGINDVIIIFDRIRENMIRSNEKIAYANVFDESIRQTFKRSLGTSISTLLVLIAMFVFGTGVIQRFSFTVGVGVVVSFLSSIFVAASTAYLLINLTKKVR" }, "paralog": false, "identity": 0.9915254237288136, "score": 437.0, "evalue": 6.25e-157, "description": "Internal stop codon at 63344, 63383. Nonsense mutation around 63344, 63383.", "psc": { "uniref90_id": "UniRef90_A0A660MWN9", "product": "Protein translocase subunit SecF", "uniref50_id": "UniRef50_A0A660MWN9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A660MWN9", "UniRef:UniRef50_A0A660MWN9" ] }, "pscc": { "uniref50_id": "UniRef50_A0A660MWN9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MWN9" ], "product": "Protein translocase subunit SecF" } }, "id": "NKOCPLAKPK_1741", "locus": "GCA003260355_01729" }, { "type": "cds", "sequence": "contig_2", "start": 63381, "stop": 63815, "strand": "-", "frame": 3, "gene": "secF", "product": "Protein translocase subunit SecF", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MWN9", "UniRef:UniRef90_A0A660MWN9" ], "nt": "ATGAGAAAGTATTTTAGTATTGTAAAAAGAGCTTATCTCTGGATTTTGATTGCTATTACTGCTACGGTAGTGTCATGGGTGTTGTTCATCTTCAGTGCACAGTTCTCAGAAGAATTTACAGGAGGAATCAGTGTCGTTTTCAATGGAAAGGTGAAAGATGCTGAATTTAAAGAACAGTTGGCACAGGGACTCGCAGAAAAAGGTTTCCCAAATCTTAAAGTCCACCTTGATAATAATGTGGGACAGACCAAAATAAAAATCAATAATAGACTCAAAGATAACGAAGAGGTAAATAAACTCTCTTTAGCGATCTCCAACTATCTTGTCGAAAGTCAGACGATTAGTTCTGAAAGTGATATTGTTGAACAGGTAGTAATAGGACCGAGTGTTGGTAAGTATATGACAAATACTGCTTCACGAGCGCTTGGCTTATGA", "aa": "MRKYFSIVKRAYLWILIAITATVVSWVLFIFSAQFSEEFTGGISVVFNGKVKDAEFKEQLAQGLAEKGFPNLKVHLDNNVGQTKIKINNRLKDNEEVNKLSLAISNYLVESQTISSESDIVEQVVIGPSVGKYMTNTASRALGL", "aa_hexdigest": "a3acf063faf1012634302e5ea23644eb", "start_type": "ATG", "rbs_motif": "TTATT", "genes": [ "secF" ], "truncated": "5-prime", "pseudogene": { "start": 63078, "stop": 63866, "observations": { "mutation": [ 63344, 63383 ], "stop": [ 63344, 63383 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A660MWN9", "query_cov": 1.0, "subject_cov": 0.43636363636363634, "identity": 1.0, "score": 271.0, "evalue": 2.99e-89, "gene_start": 1, "gene_end": 144, "reference_start": 18, "reference_end": 161, "reference_sequence": "MEGKNYSLGRFYFIFLMMRKYFSIVKRAYLWILIAITATVVSWVLFIFSAQFSEEFTGGISVVFNGKVKDAEFKEQLAQGLAEKGFPNLKVHLDNNVGQTKIKINNRLKDNEEVNKLSLAISNYLVESQTISSESDIVEQVVIGPSVGKYMTNTASRALGLGLLAMMIYMIFSFGTIRKNIAPGILATVVLFTSVLSISIPAGAYGIWMALDPTTQIDTVFIIAILTIIGYGINDVIIIFDRIRENMIRSNEKIAYANVFDESIRQTFKRSLGTSISTLLVLIAMFVFGTGVIQRFSFTVGVGVVVSFLSSIFVAASTAYLLINLTKKVR" }, "paralog": false, "identity": 0.9885496183206107, "score": 496.0, "evalue": 2.25e-180, "description": "Internal stop codon at 63344, 63383. Nonsense mutation around 63344, 63383.", "psc": { "uniref90_id": "UniRef90_A0A660MWN9", "product": "Protein translocase subunit SecF", "uniref50_id": "UniRef50_A0A660MWN9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A660MWN9", "UniRef:UniRef50_A0A660MWN9" ] }, "pscc": { "uniref50_id": "UniRef50_A0A660MWN9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MWN9" ], "product": "Protein translocase subunit SecF" } }, "id": "NKOCPLAKPK_1742", "locus": "GCA003260355_01730" }, { "type": "cds", "sequence": "contig_2", "start": 64179, "stop": 64586, "strand": "-", "frame": 3, "gene": null, "product": "Uracil-DNA glycosylase-like domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328ECS4", "UniRef:UniRef90_A0A328ECS4" ], "nt": "ATGCCCAAGTTTACAGCTTCTCATCAGTCCTTCTCCTCGGAAATCGCTTCTACTCCCCCGTATCATGGAGTCGCTTTCCTAGGACTCTCTTACAAGGAAGATAAAAAAGGAAAAATCCTGACTGCTTTCGACGAAACGACTTCTTCAGGGAAACTCATCCATTCCCTCTTGCAGTCTTCCACCAAAGAAATCGCTCTGCTGAATTTGGTGAGAGCGGTCCCCAAAGACGCAAACGGTAAATTGAGATACCCTAGCTCCAGAGAAAAGGAGGAAGGCCGAGAAATACTAAAAGAAGAGATCAAAAACTATGCTCCTCAGCTGATTTTCCTTTGTGGTAAAGAAGTTGCTGATTGCATTCTAAAGCAGCCAAAAGTGGTAAAAATTGATGACGGGCTGTATAGCTATTGA", "aa": "MPKFTASHQSFSSEIASTPPYHGVAFLGLSYKEDKKGKILTAFDETTSSGKLIHSLLQSSTKEIALLNLVRAVPKDANGKLRYPSSREKEEGREILKEEIKNYAPQLIFLCGKEVADCILKQPKVVKIDDGLYSY", "aa_hexdigest": "73ee6718951313523601327337a29378", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 64065, "stop": 64586, "observations": { "mutation": [ 64181 ], "stop": [ 64181 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328ECS4", "query_cov": 1.0, "subject_cov": 0.7803468208092486, "identity": 0.978, "score": 258.0, "evalue": 1.74e-86, "gene_start": 1, "gene_end": 135, "reference_start": 1, "reference_end": 135, "reference_sequence": "MSKFTASHQSFSSEIASTPPYHGVAFLGLSHKEDKKGKILTAFDETTSSGKLIHSLLQSSTREIALLNLVRAVPKDANGKLRYPSSREKEEGREILKEEIKNYAPQLIFLCGKEVADCILKQPKVVKIDDGLYSYGATKLLPIEHPSYIAVYRQKYKHEYIDKILTLMRKYAD" }, "paralog": false, "identity": 0.9710982658959537, "score": 334.0, "evalue": 9.08e-119, "description": "Internal stop codon at 64181. Nonsense mutation around 64181.", "psc": { "uniref90_id": "UniRef90_A0A328ECS4", "product": "Uracil-DNA glycosylase-like domain-containing protein", "uniref50_id": "UniRef50_A0A328ECS4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328ECS4", "UniRef:UniRef50_A0A328ECS4" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328ECS4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328ECS4" ], "product": "Uracil-DNA glycosylase-like domain-containing protein" } }, "id": "NKOCPLAKPK_1743", "locus": "GCA003260355_01731" }, { "type": "cds", "sequence": "contig_2", "start": 64661, "stop": 65257, "strand": "-", "frame": 1, "gene": "atpG", "product": "ATP synthase F1 subunit gamma", "db_xrefs": [ "EC:7.1.2.2", "GO:0015986", "GO:0045261", "GO:0046933", "SO:0001217", "UniRef:UniRef50_A0A3D5YEN6", "UniRef:UniRef50_A0A660MR72", "UniRef:UniRef90_A0A660MYS1" ], "nt": "ATGGAGAAAAACTACGGAAATGCGAAAGATAACGTTGATATTTTCGTTGTAGGAAAGAGAGGGCTAGAATATTTCGTGAGGAATGGACGAAACGTTGTAGGGTCTGCGATGATTTCGGATAAAGTCTTTCTCCGGGAAGTAAAACCGATCTCAGTTTTTATTAGACAAGCGTTAGAAGAGAAGAAATACGCGAAGATCAAGATCTACTTCAACTTCTTCAAAAATACGATGATACAGATGTCTACGAGATTTAAGCTCTATCCTTTAGACGAGGAAAGCTTTGAGACCTTCCTCCAAAATACAGGGCTTGAGGTCGATTTGTTAGAAAGTATCGAGAAAAAGTATTTGATGGTAGAACCTGATATAGAAACCTTTAAATTTGATTTCCTCCAAGTATTAATTAGGAATATTATCTACTCAGCAGTGCTGAATAATAAAACTTCAGAGCACGCAGCGAGAATGTTGGCGATGAAGAATGCAAAAGACAATTCACTTGCTGTCCTCAATCAGCTTAACCTCCTTTACAACAAGACCCGTCAGGCAAAAATTACTCAGGAGATCAGTGAAATCGTGAGTGCGAAGGTGGCGATTGAATAA", "aa": "MEKNYGNAKDNVDIFVVGKRGLEYFVRNGRNVVGSAMISDKVFLREVKPISVFIRQALEEKKYAKIKIYFNFFKNTMIQMSTRFKLYPLDEESFETFLQNTGLEVDLLESIEKKYLMVEPDIETFKFDFLQVLIRNIIYSAVLNNKTSEHAARMLAMKNAKDNSLAVLNQLNLLYNKTRQAKITQEISEIVSAKVAIE", "aa_hexdigest": "beffa3f066fd1c4ee8c8152da62bd960", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3D5YEN6", "query_cov": 0.8282828282828283, "subject_cov": 0.9820359281437125, "identity": 0.524, "score": 171.0, "evalue": 5.46e-51, "valid": false, "uniref50_id": "UniRef50_A0A3D5YEN6" }, "pscc": { "uniref50_id": "UniRef50_A0A3D5YEN6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3D5YEN6" ] }, "genes": [ "atpG" ], "truncated": "3-prime", "pseudogene": { "start": 64661, "stop": 65551, "observations": { "mutation": [ 65287 ], "stop": [ 65287 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A660MYS1", "query_cov": 1.0, "subject_cov": 0.668918918918919, "identity": 1.0, "score": 377.0, "evalue": 1.36e-130, "gene_start": 1, "gene_end": 198, "reference_start": 99, "reference_end": 296, "reference_sequence": "MANTKQIRTKIKSVKNLQKIIKALEIVSTVKLQKLKGVIGSYRAFMESFLEVLKEVRKEVNIFDFDEKTWNPNGRRLLIVVTSDRGLCGAINSRIFKRMEKNYGNAKDNVDIFVVGKRGLEYFVRNGRNVVGSAMISDKVFLREVKPISVFIRQALEEKKYAKIKIYFNFFKNTMIQMSTRFKLYPLDEESFETFLQNTGLEVDLLESIEKKYLMVEPDIETFKFDFLQVLIRNIIYSAVLNNKTSEHAARMLAMKNAKDNSLAVLNQLNLLYNKTRQAKITQEISEIVSAKVAIE" }, "paralog": false, "identity": 0.9966216216216216, "score": 566.0, "evalue": 2.09e-207, "description": "Internal stop codon at 65287. Nonsense mutation around 65287.", "psc": { "uniref90_id": "UniRef90_A0A660MYS1", "gene": "atpG", "product": "ATP synthase F1 subunit gamma", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_A0A660MR72", "go_ids": [ "GO:0015986", "GO:0045261", "GO:0046933" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A660MYS1", "EC:7.1.2.2", "UniRef:UniRef50_A0A660MR72", "GO:0015986", "GO:0045261", "GO:0046933" ] }, "pscc": { "uniref50_id": "UniRef50_A0A660MR72", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MR72" ], "product": "ATP synthase gamma chain" } }, "id": "NKOCPLAKPK_1744", "locus": "GCA003260355_01732" }, { "type": "cds", "sequence": "contig_2", "start": 65285, "stop": 65551, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00231.24" ], "nt": "ATGGCAAATACCAAGCAGATCAGAACCAAAATTAAATCCGTAAAAAATCTCCAAAAGATTATCAAAGCATTGGAGATTGTTTCTACCGTAAAGCTTCAAAAGCTGAAAGGCGTGATCGGTTCATATAGAGCTTTTATGGAAAGCTTTTTGGAGGTGCTAAAAGAGGTGAGAAAGGAAGTAAATATCTTTGACTTCGATGAAAAGACCTGGAACCCAAACGGAAGAAGACTCTTGATTGTGGTGACAAGTGATAGAGGGCTCTGCTGA", "aa": "MANTKQIRTKIKSVKNLQKIIKALEIVSTVKLQKLKGVIGSYRAFMESFLEVLKEVRKEVNIFDFDEKTWNPNGRRLLIVVTSDRGLC", "aa_hexdigest": "a8d68e81b61f11e24c12b1188df03439", "start_type": "ATG", "rbs_motif": "TTATT", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00231.24", "name": "ATP-synt", "length": 90, "aa_cov": 0.9772727272727273, "hmm_cov": 0.9555555555555556, "evalue": 3.1186206358014345e-20, "score": 69.45655059814453, "start": 3, "stop": 88 } ], "seq_stats": { "molecular_weight": 10166.97539999999, "isoelectric_point": 10.068301582336424 }, "id": "NKOCPLAKPK_1745", "locus": "GCA003260355_01733" }, { "type": "cds", "sequence": "contig_2", "start": 65576, "stop": 65908, "strand": "-", "frame": 1, "gene": null, "product": "ATP synthase epsilon chain", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4IJS9", "UniRef:UniRef90_A0A660MZF3" ], "nt": "GTGAGATTTACACCGATAGAAAAAAAAGGAAGTGAGTTTCTTTCTGATACGGATTTCCTTTTCGAAGATGAAAAGGTGGCAATGGCGGTCGGAGAAGGATTTATGTATACAGACGGGAACGTAGTTGTGCTTTTCGTCGCTTCTGCAACGACCAATCCAAAGTCTGATGATCAGGTGCTCCAGGAAATGAAAATTGATCTTGAAAAAAAGATCGCCGAAATCAAAGCGACAGGGGATCAAGAGGAGATTGAAAGAGCATATCTCAACCTCCAAAAATTGACCGCAGATATCCAGCTTTTGAGAATCAAAAAGAAAAAATCAGAAGGAAAGTAA", "aa": "MRFTPIEKKGSEFLSDTDFLFEDEKVAMAVGEGFMYTDGNVVVLFVASATTNPKSDDQVLQEMKIDLEKKIAEIKATGDQEEIERAYLNLQKLTADIQLLRIKKKKSEGK", "aa_hexdigest": "2589c6c180faa95c8dedf96278e3a3ea", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 65576, "stop": 66046, "observations": { "mutation": [ 65914, 65968 ], "stop": [ 65914, 65968 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A660MZF3", "query_cov": 1.0, "subject_cov": 0.7051282051282052, "identity": 0.991, "score": 207.0, "evalue": 6.54e-67, "gene_start": 1, "gene_end": 110, "reference_start": 47, "reference_end": 156, "reference_sequence": "MRLKMHTVHGVIFDGQVEKVELPSKVGVLCILPHHNPITTTTIPGIVRFTPIEKKGSEFLSDTDFLFEDEKVAMAVGEGFMYTDGNVVVLFVASATTNPKSDDQVLQEMKIDLEKKIAEIKATGDQEEIERAYLNLQKLTADIQLLRIKKKKSEGK" }, "paralog": false, "identity": 0.9871794871794872, "score": 300.0, "evalue": 3.39e-106, "description": "Internal stop codon at 65914, 65968. Nonsense mutation around 65914, 65968.", "psc": { "uniref90_id": "UniRef90_A0A660MZF3", "product": "ATP synthase epsilon chain", "uniref50_id": "UniRef50_A0A2W4IJS9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A660MZF3", "UniRef:UniRef50_A0A2W4IJS9" ] }, "pscc": { "uniref50_id": "UniRef50_A0A2W4IJS9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4IJS9" ], "product": "ATP synthase epsilon chain" } }, "id": "NKOCPLAKPK_1746", "locus": "GCA003260355_01734" }, { "type": "cds", "sequence": "contig_2", "start": 65966, "stop": 66106, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAAGCAAGTCTTCCTTGCGACAAAGATCAGATTGCTTTTATTTATCAAAGAGAGGGGAATGAGGCTAAAGATGCATACCGTTCATGGAGTAATTTTCGATGGACAGGTAGAAAAAGTTGAATTGCCAAGTAAAGTCTGA", "aa": "MKQVFLATKIRLLLFIKERGMRLKMHTVHGVIFDGQVEKVELPSKV", "aa_hexdigest": "30dcb7116f6180acc9bc7ec64a1e4e52", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5365.518500000001, "isoelectric_point": 10.282981300354002 }, "id": "NKOCPLAKPK_1747", "locus": "GCA003260355_01735" }, { "type": "cds", "sequence": "contig_2", "start": 66117, "stop": 67634, "strand": "-", "frame": 3, "gene": "atpA", "product": "F0F1 ATP synthase subunit alpha", "db_xrefs": [ "EC:7.1.2.2", "GO:0015986", "GO:0045261", "GO:0046933", "SO:0001217", "UniParc:UPI000DBFFD38", "UniRef:UniRef100_A0A328EC37", "UniRef:UniRef50_A9NGW4", "UniRef:UniRef90_A0A328EC37" ], "nt": "ATGTCAGTAGTGCAAGACTTATTACAGCAGATTGAAAAAGGGATTAACCATGCCCAGCTCAATGAGGATTTTACTAAAAAGGGAGAAATTCTGGAACTCAAAGATGGGGTGGCTACCGTGATTGGGTTAGATACGGTGATGTTTTCAGAAATTGTTCTTTTTGAAAATGGATTGAAAGGTTTAGTTTTGGATCTTTCTGCCGATGGAGTTGGTGTCCTCGTGCTCGGAGATTATAGTTCTCTTAAGCAGGGAGACAGTGTGAGTTCTTCTGGTCAGGTCTTCAGTGTTGGAGTAGGAGAAGAATACTTGGGAAGAGTGCTCAACGGAATCGGGGAACCGATCGACGGAGGAAAGGCTATCACTCCAAAGCATTTTGGGCTTGTGGAAAAAGTAGCCCCTGGAGTAATTACCAGAAAGTCAGTGAATGTTCCTTTGGAAACCGGAATTAAGTCTATTGATGCGATGGTGCCAATCGGAAGAGGGCAGAGAGAATTGATTATTGGAAATAGACAAACCGGAAAGACGACTATTGCGACCGATACGATCCTCAATCAAAAAGGAAAGAATGTCTACTGTATCTATGTCGCGATTGGACAAAAAGATTCAAAAATCAGAAGAACCGTTGAAATGCTCAGAGAGAAAGGAGCGATGGAATATACGACTGTGATCAATGCTCCGATTTCTTCTCCTTCTGTGATTCAATATATTGCTCCTTATGTAGGATGTGCAATGGGAGAATACTTTATGGAGCATGGGAAAGATGCTTTGATTATCTATGATGATCTTTCCAAGCATGCGGTAGCCTATCGTGAGGTGAGCTTGCTCCTCAGAAGGCCTCCTGGAAGAGAAGCCTATCCTGGAGATGTATTTTATCTTCATTCAAAACTGCTTGAAAGAGCTGCGAGAGTGGATCAGCCACGAGGGGGAGGAAGTTTGACGGCTTTGCCGATTATTGAGACTTTGGATTCAGATATTTCAGCCTATATTCCAACCAATGTGATTTCTATTACTGATGGGCAGATCTTTTTGGAGACAGAGCTTTTCAATTCTGGTTTCAAGCCTGCGATTGATGTGGGATTCTCAGTTTCTAGAGTAGGAGGTTCTGCTCAGACCAAAATTATGAAAAAAGTAGCTGGAAAACTCAGACTTGAACTTGCGACTTTCCGTGAATTAGCGAGCTTTTCTCAGTTTGCTTCAGATTTGGATCCTGCCACAAGAAAAAAGCTTGATAATGGAAAAAAGCTTATGGAAATGCTCAAGCAAAGCAACGATCACCCAATCCCTTTCTTCAAGCAAGTGGTGCTCCTTTATGCGGGAATTAATGGCTACTTTGAAAAAGTGCCGGTTGAGGCAATTGCTGAATTTGAGAAAAAAGTTTACCAGAAATTAGACACGAGTTATTTAGAGTTGGCAAAGAAAATCGAGGAAATTAAGGAGCTTACTTCAGAGATTGAGGCTGAGATTAAAAAGCTTATTTCAGAGATATTAAAGGAAGGAGGATTCACGAGTCAGGAATAA", "aa": "MSVVQDLLQQIEKGINHAQLNEDFTKKGEILELKDGVATVIGLDTVMFSEIVLFENGLKGLVLDLSADGVGVLVLGDYSSLKQGDSVSSSGQVFSVGVGEEYLGRVLNGIGEPIDGGKAITPKHFGLVEKVAPGVITRKSVNVPLETGIKSIDAMVPIGRGQRELIIGNRQTGKTTIATDTILNQKGKNVYCIYVAIGQKDSKIRRTVEMLREKGAMEYTTVINAPISSPSVIQYIAPYVGCAMGEYFMEHGKDALIIYDDLSKHAVAYREVSLLLRRPPGREAYPGDVFYLHSKLLERAARVDQPRGGGSLTALPIIETLDSDISAYIPTNVISITDGQIFLETELFNSGFKPAIDVGFSVSRVGGSAQTKIMKKVAGKLRLELATFRELASFSQFASDLDPATRKKLDNGKKLMEMLKQSNDHPIPFFKQVVLLYAGINGYFEKVPVEAIAEFEKKVYQKLDTSYLELAKKIEEIKELTSEIEAEIKKLISEILKEGGFTSQE", "aa_hexdigest": "4fb5d341172de34860ed33bca6840cba", "start_type": "ATG", "rbs_motif": "TTTTTA", "ups": { "uniparc_id": "UPI000DBFFD38", "uniref100_id": "UniRef100_A0A328EC37", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBFFD38", "UniRef:UniRef100_A0A328EC37" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EC37", "uniref90_id": "UniRef90_A0A328EC37" }, "psc": { "uniref90_id": "UniRef90_A0A328EC37", "gene": "atpA", "product": "F0F1 ATP synthase subunit alpha", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_A9NGW4", "go_ids": [ "GO:0015986", "GO:0045261", "GO:0046933" ] }, "pscc": { "uniref50_id": "UniRef50_A9NGW4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A9NGW4" ], "product": "ATP synthase subunit alpha" }, "genes": [ "atpA" ], "id": "NKOCPLAKPK_1748", "locus": "GCA003260355_01736" }, { "type": "cds", "sequence": "contig_2", "start": 67765, "stop": 68217, "strand": "-", "frame": 2, "gene": null, "product": "ATP synthase subunit delta", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DC03AE4", "UniRef:UniRef100_A0A328EE99", "UniRef:UniRef50_A0A2W4IIM9", "UniRef:UniRef90_A0A660MYT7" ], "nt": "ATGCTAAACAAGAAACAACTCCTTGAACAGCTGCTCCAGATGAAACATCCTCAGAAACTTCTGGAAAAGTTGGCCGTCATTAGAGCCTTACTTCTCAAATATGGTAAGAAATTGACACTCCCTCGCTTCTTAGAGGGGATAAAAAATGGGGATGCTGATACAGCGAGTATGATTCATCTCCTCGAACAAGCAAAGTTACTTCACTTAGCAAAGTTATCCGCATTTATTCGTGAGTTTCAGGAAAAGCTACCGAGAGAGCATCTCCAGTTTCGTTTGGAATCGAATGATGAGGATATTATTGTTCCTCTGACAGCCTATCTTGAAGAAAAGTTTGGAAAGGTTGAGGTGGCGTTTCATCCACTTGCCTCAGAGAATTTAACTGTAAAGATGAAAGGGCAGGGCTATATCTTCAAGAGGTCACTTGAAAAAGACTTAGAAGTATTACTTGAATAA", "aa": "MLNKKQLLEQLLQMKHPQKLLEKLAVIRALLLKYGKKLTLPRFLEGIKNGDADTASMIHLLEQAKLLHLAKLSAFIREFQEKLPREHLQFRLESNDEDIIVPLTAYLEEKFGKVEVAFHPLASENLTVKMKGQGYIFKRSLEKDLEVLLE", "aa_hexdigest": "3091ae2324fc9f06e92cb0ccc98b7548", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000DC03AE4", "uniref100_id": "UniRef100_A0A328EE99", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DC03AE4", "UniRef:UniRef100_A0A328EE99" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EE99", "uniref90_id": "UniRef90_A0A660MYT7" }, "psc": { "uniref90_id": "UniRef90_A0A660MYT7", "product": "ATP synthase subunit delta", "uniref50_id": "UniRef50_A0A2W4IIM9" }, "pscc": { "uniref50_id": "UniRef50_A0A2W4IIM9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4IIM9" ], "product": "F-type ATPase subunit delta" }, "genes": [], "id": "NKOCPLAKPK_1749", "locus": "GCA003260355_01737" }, { "type": "cds", "sequence": "contig_2", "start": 68249, "stop": 68584, "strand": "-", "frame": 1, "gene": null, "product": "ASCH domain-containing protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DC04D16", "UniRef:UniRef100_A0A328ECU1", "UniRef:UniRef50_A0A328ECU1", "UniRef:UniRef90_A0A328ECU1" ], "nt": "ATGAAGACGATCTATTTTACGGTCGGGGAGCCTTACAAATCCTTGATCCTTCAAGGAAAGAAAACGGTAGAAGGGAGACTTTGCAAAGGAAAGTTTTCCGAACTTCAAGTCGGCGACTTTCTCCAGTTTCAAGATACAGGAGAATGTTTAGAGATTATTGCTTTGACACACTACCCTAGTTTCAAGGCAATGCTAGAAAATGAAGGACTCAAGCATACGCTTCCGGGAATTACTGAGATTGATCATGGGAGAGAGGTATATTACCAGTTTTATACTCCAGAAGAAGAAAAAACATTTGGCGTCCTTGCAATCTCCATCCGTCTAAAAACAACCTAA", "aa": "MKTIYFTVGEPYKSLILQGKKTVEGRLCKGKFSELQVGDFLQFQDTGECLEIIALTHYPSFKAMLENEGLKHTLPGITEIDHGREVYYQFYTPEEEKTFGVLAISIRLKTT", "aa_hexdigest": "509c6d67d6a6ddeac720250a66eef750", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000DC04D16", "uniref100_id": "UniRef100_A0A328ECU1", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DC04D16", "UniRef:UniRef100_A0A328ECU1" ] }, "ips": { "uniref100_id": "UniRef100_A0A328ECU1", "uniref90_id": "UniRef90_A0A328ECU1" }, "psc": { "uniref90_id": "UniRef90_A0A328ECU1", "product": "ASCH domain-containing protein", "uniref50_id": "UniRef50_A0A328ECU1" }, "pscc": { "uniref50_id": "UniRef50_A0A328ECU1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328ECU1" ], "product": "ASCH domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_1750", "locus": "GCA003260355_01738" }, { "type": "cds", "sequence": "contig_2", "start": 68839, "stop": 68949, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGAACTTCCTTTTGACTTTACTGGTTATGTAGAAAAAGGAATCGACAATCAGCTTCAAAAAGCACAAGAAATACTTGCTGAAAAAATCACTAAAGTAGCAGAACAATAA", "aa": "MELPFDFTGYVEKGIDNQLQKAQEILAEKITKVAEQ", "aa_hexdigest": "7a809e50e0aa83470a835aab09c9de33", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4125.652400000001, "isoelectric_point": 4.4943956375122065 }, "id": "NKOCPLAKPK_1751", "locus": "GCA003260355_01739" }, { "type": "cds", "sequence": "contig_2", "start": 69190, "stop": 69432, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTTGGATATCTCGAAGTGTCTATTTTTGGAGATCAAACCAATAGGCTCTTTACTAGAGCAATTTCAGAGCTTCTTGAAGCTAAAGTAAAAGGGATCATCCTTGATCTAAGAGGAAATGGTGGCGGACTACTAACCAGTGCGGTGGAGCTTGCTGGACACTTCTTACCAAAAGGGACTCTTATTACGAAGACAAAATATTCCAATTTTAAGCCAATAGATTATCAATCAAAAGGTTTTTGA", "aa": "MLGYLEVSIFGDQTNRLFTRAISELLEAKVKGIILDLRGNGGGLLTSAVELAGHFLPKGTLITKTKYSNFKPIDYQSKGF", "aa_hexdigest": "8522829bfe01b725d05b761e46d6880f", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8763.081499999995, "isoelectric_point": 9.397121238708493 }, "id": "NKOCPLAKPK_1752", "locus": "GCA003260355_01740" }, { "type": "cds", "sequence": "contig_2", "start": 69436, "stop": 69690, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGTCAAAGATTCTCCTGCTTTCAAAGCGGGTGTTCTGCCATTAGACAGAATTATTATGATCGGGACGGGAGAGACAAAGGATCTTAGCACTATGGAGGCGGTTCAAAAAATCAGGGGACCAAAAGGAACGAAGGTTCAGCTTTTTCTTGAGAGAGTTGAGAAAACCGGTGAGAAATCTTATCTCGAAAAGGTTGTAGAAAGAGGTATCATTGATGTTCCTTCTGTGAGATCAAAAATTCTGAATCAAAGCTGA", "aa": "MVKDSPAFKAGVLPLDRIIMIGTGETKDLSTMEAVQKIRGPKGTKVQLFLERVEKTGEKSYLEKVVERGIIDVPSVRSKILNQS", "aa_hexdigest": "35259f90f9af0ca1d36613a830a6f219", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9299.835699999996, "isoelectric_point": 9.570863533020017 }, "id": "NKOCPLAKPK_1753", "locus": "GCA003260355_01741" }, { "type": "cds", "sequence": "contig_2", "start": 69733, "stop": 69933, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAACAAATTTCTCAGCTTTTATCGGGAGAATATTATGACAAGGAAGAATTCCTCAGTGGTCAGAAAAATATGATCGAAAATGCGACCAAGGCATTCGTTGATGGGTTGGGAGATCCTTATACTTCCTATCTTGACGCAGAGCAATTTTCAGGACTCCAAACAGAACTTGAAGGAGAAGGACAAATAGAGGGAATCTGA", "aa": "MEQISQLLSGEYYDKEEFLSGQKNMIENATKAFVDGLGDPYTSYLDAEQFSGLQTELEGEGQIEGI", "aa_hexdigest": "93308abc8d7ce3a7d4471c4efcafe972", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7356.9304999999995, "isoelectric_point": 4.0500284194946286 }, "id": "NKOCPLAKPK_1754", "locus": "GCA003260355_01742" }, { "type": "cds", "sequence": "contig_2", "start": 70253, "stop": 70441, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTGCTCCAAAGGATCAAAAGCTAAAATACCAAGCGACAAAGCTAGTGAATGCTCGAATCCCTATTCCTCGCCAAAAAAGCTCCCGCCTCCAGCGAAACGAACCATTAAATCCAACCAAAAAGAGGAAGGTCGGAAACGCAATTCTTCCAATCAATCTCAAGACCAATACTTCTGGGAAAAGATAA", "aa": "MFAPKDQKLKYQATKLVNARIPIPRQKSSRLQRNEPLNPTKKRKVGNAILPINLKTNTSGKR", "aa_hexdigest": "a454af125c4d1cb30ff54c3e8371ff7b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7096.320200000001, "isoelectric_point": 11.758984947204592 }, "id": "NKOCPLAKPK_1755", "locus": "GCA003260355_01743" }, { "type": "cds", "sequence": "contig_2", "start": 71272, "stop": 71523, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAACTTTTAGCCCATATCCTTACCAATTTTGGGATAATAATTGATCTAAAAATGATCAGCAGCGGAAAGATTATCTTAAAGGAGAAGGTAAAACATTTTGTAGAAATTGATGAAGGCAATATGCAAGATGTATCGGTTCTGACAAAAATTGGATATATAGATGGACTAGCAATAGTTGGGCCACAAAGTCAGAAGCAGGTTATACTCTATTTAGAGAAAAGAAAGAAGATGATTTTAAAGTGTTAA", "aa": "MKKLLAHILTNFGIIIDLKMISSGKIILKEKVKHFVEIDEGNMQDVSVLTKIGYIDGLAIVGPQSQKQVILYLEKRKKMILKC", "aa_hexdigest": "fe1e1700f6eb3edafaddd54fca4ac0a7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9410.374599999997, "isoelectric_point": 9.590332984924316 }, "id": "NKOCPLAKPK_1756", "locus": "GCA003260355_01744" }, { "type": "cds", "sequence": "contig_2", "start": 72466, "stop": 72819, "strand": "-", "frame": 2, "gene": null, "product": "Peptidase C51 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBFEA60", "UniRef:UniRef100_A0A328ECT1", "UniRef:UniRef50_A0A328ECT1", "UniRef:UniRef90_A0A328ECT1" ], "nt": "ATGTGGGGAATTGAAAATGGCTTCTATGGTAAAGCTAATAGTAATTCCTTAGGAAATGCAAAAGATTGGGAGTCTAAGCTACTTCTTAAAGGTTATTCTGCGGATTTGAATCCAAAGGTCGATGATATTGCGTGGTGGGAGTCAAATGTTGATGGGAATTCAGGAGCCAATGGTCACGTAGCTTTCGTAAATGAGGTAAGAGATGGTGGTAATACTGTTGTAATTACTGAGTATAACTTTCTCAGTCTGAATGCATACGAATCCCCAGAAAGAGTATTGCAAAGGTATGGGGCTGCGCAAAATAGTAGGAAGTTCGCATCTTCCTTTATTCATGTACAGACAAAACGGCAATAG", "aa": "MWGIENGFYGKANSNSLGNAKDWESKLLLKGYSADLNPKVDDIAWWESNVDGNSGANGHVAFVNEVRDGGNTVVITEYNFLSLNAYESPERVLQRYGAAQNSRKFASSFIHVQTKRQ", "aa_hexdigest": "657560e5d35e5e129ce5ecf4f671c740", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000DBFEA60", "uniref100_id": "UniRef100_A0A328ECT1", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBFEA60", "UniRef:UniRef100_A0A328ECT1" ] }, "ips": { "uniref100_id": "UniRef100_A0A328ECT1", "uniref90_id": "UniRef90_A0A328ECT1" }, "psc": { "uniref90_id": "UniRef90_A0A328ECT1", "product": "Peptidase C51 domain-containing protein", "uniref50_id": "UniRef50_A0A328ECT1" }, "pscc": { "uniref50_id": "UniRef50_A0A328ECT1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328ECT1" ], "product": "Peptidase C51 domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_1757", "locus": "GCA003260355_01745" }, { "type": "cds", "sequence": "contig_2", "start": 72892, "stop": 73011, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGCTATGCTTGACAAAAATAAAGTTCAAGAAGATGTTCATTTTTCTGTAGAAGATAAAAAAAAATCTGAGGATAAAGATCAATCTTCCCTAATGACTCAGATTTTTCAGTCTTTTTAA", "aa": "MAMLDKNKVQEDVHFSVEDKKKSEDKDQSSLMTQIFQSF", "aa_hexdigest": "33bc4ad177665f57146327b2460ead31", "start_type": "GTG", "rbs_motif": "TTTA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4593.130299999999, "isoelectric_point": 5.037946891784667 }, "id": "NKOCPLAKPK_1758", "locus": "GCA003260355_01746" }, { "type": "cds", "sequence": "contig_2", "start": 73290, "stop": 73460, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATGCTCAATCATATGATATGTATTTCAGAAATAAATTTTTCACACATTTAGTGAATTTTATTTTTTTTCACTATTTCTATGAATTTTTCCCAATAAAAAACCACAAACCAATTATAAACCCTTATTATTTTTTTGCAAATATTTTCATACTTTCTATGAAACAGTGA", "aa": "MDAQSYDMYFRNKFFTHLVNFIFFHYFYEFFPIKNHKPIINPYYFFANIFILSMKQ", "aa_hexdigest": "23d9d75b151d03a50f4fe3d427920d1f", "start_type": "ATG", "rbs_motif": "TTTTT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7136.232799999998, "isoelectric_point": 9.050732612609863 }, "id": "NKOCPLAKPK_1759", "locus": "GCA003260355_01747" }, { "type": "cds", "sequence": "contig_2", "start": 73675, "stop": 73953, "strand": "-", "frame": 2, "gene": null, "product": "Pseudouridine synthase RsuA/RluA-like domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EC45", "UniRef:UniRef90_A0A328EC45" ], "nt": "GTGAATTATAAAACTCTTCAACAAGCGGGAATGGTTCATAAGTATTATTTGCTTGAGGTCTATGGAGATATTAAGTATCGGGTAGAAAAGAATGGGTTAGTGATTGATTTTCCAATTGCTCATCACAAATTTGCTCCTGAAAAAATGGTAGTAATTGATAATGAAGATAAAAAAAATAAAGCTAATATGAGGTGTCATCGTGTGCAAACAGAAATTGTAGAGTTTCAATGGAATGAGTTGAGTAAAACTACAACTCTACTCGTAAAAATCAAGAAATGA", "aa": "MNYKTLQQAGMVHKYYLLEVYGDIKYRVEKNGLVIDFPIAHHKFAPEKMVVIDNEDKKNKANMRCHRVQTEIVEFQWNELSKTTTLLVKIKK", "aa_hexdigest": "8f95b9a255469469a84737d951417456", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 73540, "stop": 74202, "observations": { "mutation": [ 73584, 73677, 73989, 74079, 74094, 74151 ], "stop": [ 73584, 73677, 73989, 74079, 74094, 74151 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EC45", "query_cov": 1.0, "subject_cov": 0.41818181818181815, "identity": 0.9890000000000001, "score": 188.0, "evalue": 6.86e-59, "gene_start": 1, "gene_end": 92, "reference_start": 84, "reference_end": 175, "reference_sequence": "MKVIRYEDEFCYYVRKPGGIPTTFGKEKSFLEYLFEGSGKSGSYPFISLLSKAFTEDEERGLLNRLDNATSGLLYFAKTREIKVNYKTLQQAGMVHKYYLLEVYGDIKYRVEKNGLVIDFPIAHHKFAPEKMVVIDNEDKKNKANMRCHRVQTEIVEFQWNELSKTTTLLVKIKKGIRHQIRSHLSVIGYPIVGDELYGKKKDSKRGNLQLFSVGLSVKG" }, "paralog": false, "identity": 0.9727272727272728, "score": 422.0, "evalue": 9.51e-154, "description": "Internal stop codon at 73584, 73677, 73989, 74079, 74094, 74151. Nonsense mutation around 73584, 73677, 73989, 74079, 74094, 74151.", "psc": { "uniref90_id": "UniRef90_A0A328EC45", "product": "Pseudouridine synthase RsuA/RluA-like domain-containing protein", "uniref50_id": "UniRef50_A0A328EC45", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EC45", "UniRef:UniRef50_A0A328EC45" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EC45", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EC45" ], "product": "Pseudouridine synthase RsuA/RluA-like domain-containing protein" } }, "id": "NKOCPLAKPK_1760", "locus": "GCA003260355_01748" }, { "type": "cds", "sequence": "contig_2", "start": 73950, "stop": 74180, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATTTTGCTACTATGTCCGAAAGCCCTGAGGTATTCCAACAACTTTTGGAAAAGAAAAATCCTTTCTTGAATATCTCTTTGAATGATCGGGAAAAAGCTGAAGTTACCCATTTATTAGCCTACTTTCCAAGGCTTTCACCGAAGATGAAGAACGAGGGCTTCTTAATAGATTGGATAATGCGACATCATGACTTCTCTATTTTGCGAAAACTCGTGAAATTAAAGTGA", "aa": "MNFATMSESPEVFQQLLEKKNPFLNISLNDREKAEVTHLLAYFPRLSPKMKNEGFLIDWIMRHHDFSILRKLVKLK", "aa_hexdigest": "78d6858f614e712f1353d2281bd0684d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9074.578999999994, "isoelectric_point": 9.45404682159424 }, "id": "NKOCPLAKPK_1761", "locus": "GCA003260355_01749" }, { "type": "cds", "sequence": "contig_2", "start": 74293, "stop": 75114, "strand": "-", "frame": 2, "gene": "atpD", "product": "F0F1 ATP synthase subunit beta", "db_xrefs": [ "EC:7.1.2.2", "GO:0015986", "GO:0045261", "GO:0046933", "SO:0001217", "UniRef:UniRef50_P29707", "UniRef:UniRef90_A0A660MYS7" ], "nt": "ATGAAGGAATCTGGGGTACTCGATAAAACAGCGATGGTTTATGGACAGATGAACGAAACTCCTGGTCCAAGAGCGAGAGTAGCTTTGACAGGATTGACAATGGCAGAATACTTTAGAGATAAAGAGGGAAAAGATGTGTTGCTCTTTATTGATAATATCTTCAGATTTACTCAGGCAGGATCTGAGGTTTCAGCTTTGCTTGGAAGAATCCCTTCTGCTGTAGGATACCAGCCAACCTTAGCGAGTGAGATGGGAACTTTGCAAGAGAGAATTACTTCTACGGATAAAGGGTCTATTACTTCGGTGCAGGCGGTGTATGTTCCTGCGGATGATCTTACGGATCCTGCTCCTGCAACGACTTTTACGCATCTTGATTCTACGATCGTGTTGAATAGGTCTATCGCTGAAAAGGGGATTTACCCGGCAGTGGATCCGCTTGAGAGTTATTCTCTGATTTTGAATCCTGATGCAGTAGGAGAAAGGCACTATAAAGTAGCCAAAGAAATCCAAAGAATCCTCCAAAAATATAAGGAACTTCAAGACATTATCGCTATTCTCGGTATGGAAGAACTTGGAGTGGAAGACAAGGTAACGGTTTATAGAGCCAGAAAAATTGAAAAGTTCCTTTCCCAGTCCTTCTCGGTGGCTGAGCAGTTTACCGGAATGCCAGGAACTTATGTAAAACTTGAGGATACCATTGCGGGATTTGAGAAAATCATTAATGGTGATGTGGATGAAATCCCTGAAGAATACTTTATGTATAAAGGAACTTTGCAGGAGGTAATTGAAGCGTTTGATAAAGATCAAAAAAAAGAAGAATAA", "aa": "MKESGVLDKTAMVYGQMNETPGPRARVALTGLTMAEYFRDKEGKDVLLFIDNIFRFTQAGSEVSALLGRIPSAVGYQPTLASEMGTLQERITSTDKGSITSVQAVYVPADDLTDPAPATTFTHLDSTIVLNRSIAEKGIYPAVDPLESYSLILNPDAVGERHYKVAKEIQRILQKYKELQDIIAILGMEELGVEDKVTVYRARKIEKFLSQSFSVAEQFTGMPGTYVKLEDTIAGFEKIINGDVDEIPEEYFMYKGTLQEVIEAFDKDQKKEE", "aa_hexdigest": "bb6952ae650dc403b36a1465da8cfa20", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A2W4KAD2", "query_cov": 0.9963369963369964, "subject_cov": 0.8888888888888888, "identity": 0.757, "score": 414.0, "evalue": 9.44e-144, "valid": false, "gene": "atpD", "product": "F0F1 ATP synthase subunit beta", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_A0A0J7YM04", "go_ids": [ "GO:0015986", "GO:0045261", "GO:0046933" ] }, "genes": [ "atpD" ], "truncated": "3-prime", "pseudogene": { "start": 74293, "stop": 75414, "observations": { "mutation": [ 75177, 75255, 75384 ], "stop": [ 75177, 75255, 75384 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A660MYS7", "query_cov": 1.0, "subject_cov": 0.5845824411134903, "identity": 0.9670000000000001, "score": 515.0, "evalue": 3.76e-181, "gene_start": 1, "gene_end": 273, "reference_start": 195, "reference_end": 467, "reference_sequence": "MKGKIVSVKGVVVEVLFEQEAPKVYDALKVVPKNANGNEVVIEVLQILENGIVRGIAMESTDGLKRGDEVENTKSPIKVPVGPEVLGHIFNVLGKTIDGTEQIAGSEQWPIHRKPPVFADLATRAEILETGIKVVDLLAPILKGGKVGLFGGAGVGKTVVMMELIHNIGTEHSGVSVVAGVGERTREGNDLYHEMKESGVLDKTAMVYGQMNETPGPRARVALTGLTMAEYFRDQEGKDVLLFIDNIFRFTQAGSEVSALLGRIPSAVGYQPTLASEMGTLQERITSTDKGSITSVQAVYVPADDLTDPAPATTFTHLDSTIVLNRSIAEKGIYPAVDPLESYSLILNPDAVGERHYKVAKEIQRILQKYKELQDIIAILGMEELGVEDKVTVYRARKIEKFLSQSFSVAEQFTGMPGTYVKLEDTIAGFERIINGEVDEIPEEFFMYKGTLQEVIDAYQADQKKAE" }, "paralog": false, "identity": 0.9571045576407506, "score": 700.0, "evalue": 2.28e-256, "description": "Internal stop codon at 75177, 75255, 75384. Nonsense mutation around 75177, 75255, 75384.", "psc": { "uniref90_id": "UniRef90_A0A660MYS7", "gene": "atpD", "product": "F0F1 ATP synthase subunit beta", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_P29707", "go_ids": [ "GO:0015986", "GO:0045261", "GO:0046933" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A660MYS7", "EC:7.1.2.2", "UniRef:UniRef50_P29707", "GO:0015986", "GO:0045261", "GO:0046933" ] }, "pscc": { "uniref50_id": "UniRef50_P29707", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P29707" ], "product": "ATP synthase subunit beta, sodium ion specific" } }, "id": "NKOCPLAKPK_1762", "locus": "GCA003260355_01750" }, { "type": "cds", "sequence": "contig_2", "start": 75382, "stop": 75696, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02874.29" ], "nt": "ATGAACGGAAAAATTGTTTCAGTAAAAGGTGTGGTGGTTGAGGTGCTTTTTGAAGAGGAATCACCAAAGGTGTATGATGCTCTCAAGGTAAAACCAAAGAATGCGAATGGAAATGAGGTTGTAATTGAAGTTTTACAGATTCTAGAGAATGGAATCGTGAGGGGAATCGCAATGGAGTCTACTGATGGACTAAAAAGAGGTGATGAAGTCGAAAATACCAAAAATCCGATTCAAGTCCCTGTTGGACCTCAGGTTTTGGGGCATATTTTCAATGTACTTGGAAAGACGATTGATGGAAGCGACCAGATTGCTTGA", "aa": "MNGKIVSVKGVVVEVLFEEESPKVYDALKVKPKNANGNEVVIEVLQILENGIVRGIAMESTDGLKRGDEVENTKNPIQVPVGPQVLGHIFNVLGKTIDGSDQIA", "aa_hexdigest": "f1c5bedfd1126e9523eee9152f22bff7", "start_type": "ATG", "rbs_motif": "TTTA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02874.29", "name": "ATP-synt_ab_N", "length": 70, "aa_cov": 0.6634615384615384, "hmm_cov": 0.9714285714285714, "evalue": 2.038811807627371e-15, "score": 53.52763748168945, "start": 5, "stop": 73 } ], "seq_stats": { "molecular_weight": 11177.72549999999, "isoelectric_point": 4.933419990539549 }, "id": "NKOCPLAKPK_1763", "locus": "GCA003260355_01751" }, { "type": "cds", "sequence": "contig_2", "start": 75941, "stop": 76156, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAAATCTTTTATAGATAATAGTACTGAAATTTTTGCTTCGAAAGAAATAGATCTTTCTAATTATCCAAACGGTAATCATAGCTTGGCAATTACAGCAATTGATGAGGCTGGAGCTACAAAAACAGTAAGTACGCAAATTAATCTCGTTTCAGAAGATAAGACCCCGCCAGTTTTTAGAAAAGAGCAAAGCAGGGTAGAAAAAACTGAAGACTGA", "aa": "MKSFIDNSTEIFASKEIDLSNYPNGNHSLAITAIDEAGATKTVSTQINLVSEDKTPPVFRKEQSRVEKTED", "aa_hexdigest": "a87446284e99f413747b4e16cb8af1b2", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7858.582600000002, "isoelectric_point": 4.837873649597167 }, "id": "NKOCPLAKPK_1764", "locus": "GCA003260355_01752" }, { "type": "cds", "sequence": "contig_2", "start": 76526, "stop": 76783, "strand": "-", "frame": 1, "gene": null, "product": "Penicillin-binding protein transpeptidase domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3D0UY96" ], "nt": "ATGTGGGCTGGAAATGCAAATGCTACTCCTATGAACAGAAATGCTTATGGAGGAACGATCCATGCAAACAGTATAAAGTCATTTCTCTCAAGCTTATTAGAACATAACTATCTTACCAACGAGGAAATGGCAAATAAAGATACAACAAGCTTGAGTATTTCTCTTATTTCTGGAAAAATTCCTGGAGACACTACTCCTCCTAGCCTTGTTGGACAGACGCTCGGATGGAATGGAAATCTCCCAAAAGAAGCGGAATGA", "aa": "MWAGNANATPMNRNAYGGTIHANSIKSFLSSLLEHNYLTNEEMANKDTTSLSISLISGKIPGDTTPPSLVGQTLGWNGNLPKEAE", "aa_hexdigest": "3abab9ad6390d4b6bbe246118c806b26", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3D0UY96", "query_cov": 0.8705882352941177, "subject_cov": 0.925, "identity": 0.5539999999999999, "score": 84.0, "evalue": 1.3e-19, "valid": false, "product": "Penicillin-binding protein transpeptidase domain-containing protein", "uniref50_id": "UniRef50_A0A3D0UY96" }, "pscc": { "uniref50_id": "UniRef50_A0A3D0UY96", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3D0UY96" ], "product": "Penicillin-binding protein transpeptidase domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_1765", "locus": "GCA003260355_01753" }, { "type": "cds", "sequence": "contig_2", "start": 76865, "stop": 76957, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTGGAAAATTCTTTCTGATCCAAATAACAGACTAGGAGGTTGGGTGACAAAGTTTAATGTTAAAGGATTGACATATGCTCTAAAAACCTGA", "aa": "MWKILSDPNNRLGGWVTKFNVKGLTYALKT", "aa_hexdigest": "1c847d28dd8c66418efb750d90ee6b80", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3452.034200000002, "isoelectric_point": 10.171064186096192 }, "id": "NKOCPLAKPK_1766", "locus": "GCA003260355_01754" }, { "type": "cds", "sequence": "contig_2", "start": 76970, "stop": 77113, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGGAGCTTGCAGGTGCCTACGAACACCTTTCTACTCCGACGCCTGCCGAAATAAATCCAATTCTTGAGATTAAGGCAAGTGATGGCAAGATCCTCTACAAAAAAGAGGTAAAGACAAAACAAAATGTCATAAAACCATGA", "aa": "MLELAGAYEHLSTPTPAEINPILEIKASDGKILYKKEVKTKQNVIKP", "aa_hexdigest": "8d7c7ded7ac7ee78c32783f25b72291d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5221.074200000003, "isoelectric_point": 8.03052043914795 }, "id": "NKOCPLAKPK_1767", "locus": "GCA003260355_01755" }, { "type": "cds", "sequence": "contig_2", "start": 77129, "stop": 77626, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00905.27" ], "nt": "TTGGATTATGAAGTTCAGAAGCTAGCAGAAACAGCACTTACGAATAATAAGTCATCTTTCTTTGAGAATGGTGCAACAAATGGATCTATGCTTTACTTAGATTCTCATAATGGGGATGTGCTTGCGTATGTTGGATCTCTTGATTATTTTAATAAAGAAATCCAGGGACAGAACGATATGGTAAGAAATCCTAGGCAGTCTGGATCTTCAATAAAACCTCTGGTGTATGCACTAGGTTTTGATAAGCTTCCTCTTACGATAGATACTCCAATTTATGATATTTTATTCAGGGCAGGAAAGGATACTCCTAATAATGCTGATGGAAAGTTTGATGGAATGCTTCCTCTCAAAAAGGCTCTTGGATTCAGTAGAAATATCCCCGCAGTAAAGATGTTCTTTGCAGTTGGAGGAGAGGATGTAGTAAAACCATTTCTCCAGAGTTTAGGACTTTCTGGTATCAAGAACAATATCTCTTACGGATATCCTCTTGCTTTATGA", "aa": "MDYEVQKLAETALTNNKSSFFENGATNGSMLYLDSHNGDVLAYVGSLDYFNKEIQGQNDMVRNPRQSGSSIKPLVYALGFDKLPLTIDTPIYDILFRAGKDTPNNADGKFDGMLPLKKALGFSRNIPAVKMFFAVGGEDVVKPFLQSLGLSGIKNNISYGYPLAL", "aa_hexdigest": "feb44ebb42590d9d35ea6cf9e8b2e5af", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00905.27", "name": "Transpeptidase", "length": 129, "aa_cov": 0.7454545454545455, "hmm_cov": 0.9922480620155039, "evalue": 2.9609056521559037e-08, "score": 29.659528732299805, "start": 28, "stop": 150 } ], "seq_stats": { "molecular_weight": 18069.36689999998, "isoelectric_point": 5.782253456115721 }, "id": "NKOCPLAKPK_1768", "locus": "GCA003260355_01756" }, { "type": "cds", "sequence": "contig_2", "start": 77663, "stop": 77815, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTCGATGATGGAAAGATTACAGAAGAGGAAGTAAAAGCAGCTATTATAGAGTCTTTTACCAAAAATTTTGAAAAAAGTGAGTTTGATATTAAGGCACCTCATTTTATTTTCTGGATTAAGGATCTTATTGAAAAAGAATATGGAACCTGA", "aa": "MFDDGKITEEEVKAAIIESFTKNFEKSEFDIKAPHFIFWIKDLIEKEYGT", "aa_hexdigest": "7c58af42a30a1f985a1246b80d76abb4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5929.656399999999, "isoelectric_point": 4.637345695495605 }, "id": "NKOCPLAKPK_1769", "locus": "GCA003260355_01757" }, { "type": "cds", "sequence": "contig_2", "start": 77819, "stop": 78397, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00912.28" ], "nt": "ATGAAGCTGGAAAGACCGTTTGGAGGTAGTTTTCTTGATAAGGTAGATTATAAATTAAAGCAGATTGTCCTCGCTAAAAGGCTTAATAATGTTCTACAAAAACAGATAAAACTAGAAAATAAAAGTCTGAATAGTGAGCAAGTTAAACATGAAATGAAAAATAAGGTTCTTGAGCTTTATCTCAATTATGTTTTCTTAGGAAATAATGCTTATGGAGTTGAAGCTGCAAGTAAGACCTATTTCAGCAAATCAGCAAAAAATTTGACAGTTCTAGAATCAGCAATTCTTGCTTCGATTCCCAAGGCTCCTACAAGATATGATCCCTATAAATCTCACCTATTGCTAGGATCCTTCAAGGTTGTTGATACGAGTGGTCAAGAGTATCCATTTGAAGGAAATATTAAATCTGCCGTCTTGACAAAATATAGAGAAAATTTACTACAGGCTAATCTTGCATCAAAGAAAAATGCGGAAGTTCTTGTGAAGTTTATTATAGGACTTTGTCCTTCAGAGATAAGCTTGGATGGAAAAACTTATCGTATCAGTTATGAAAATGGAAGAGCCGAATTTGTAATTTGA", "aa": "MKLERPFGGSFLDKVDYKLKQIVLAKRLNNVLQKQIKLENKSLNSEQVKHEMKNKVLELYLNYVFLGNNAYGVEAASKTYFSKSAKNLTVLESAILASIPKAPTRYDPYKSHLLLGSFKVVDTSGQEYPFEGNIKSAVLTKYRENLLQANLASKKNAEVLVKFIIGLCPSEISLDGKTYRISYENGRAEFVI", "aa_hexdigest": "a601ab574352a34fa6bc282f230ec687", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00912.28", "name": "Transgly", "length": 75, "aa_cov": 0.390625, "hmm_cov": 1.0, "evalue": 4.591886314759584e-22, "score": 74.74183654785156, "start": 37, "stop": 111 } ], "seq_stats": { "molecular_weight": 21689.87320000001, "isoelectric_point": 9.503816413879395 }, "id": "NKOCPLAKPK_1770", "locus": "GCA003260355_01758" }, { "type": "cds", "sequence": "contig_2", "start": 78434, "stop": 78757, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00912.28" ], "nt": "ATGGTGGCAGGGCTGGTAGTTCTTGGAATTTTTTCAGGATTTGCGTATTTACAGTTAAGAATACTAAATAACCTTCCTGATGTAAGCCAAATAAAAAATATGACAATGGTGCAGTCGACAATTATCACCGACAGAAATGGTCACGAGCTCTACAAGATTTTTGACGAAAATAGAGAATATGTTGATTTAAAAAACATTAGTCAACATATGATTGATGCTATTGTTTCAGTAGAGGACCAAAGATTTTGGGAGCATGAAGGATTAGATCCAATGGGGATTTTCAGAGCCGGATTTAGGACTATGCTAGGACAGAATGGAGGGTGA", "aa": "MVAGLVVLGIFSGFAYLQLRILNNLPDVSQIKNMTMVQSTIITDRNGHELYKIFDENREYVDLKNISQHMIDAIVSVEDQRFWEHEGLDPMGIFRAGFRTMLGQNGG", "aa_hexdigest": "e42ea04c2a0c740f0c2ecd2812806e9f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00912.28", "name": "Transgly", "length": 58, "aa_cov": 0.5327102803738317, "hmm_cov": 1.0, "evalue": 1.875703142543572e-15, "score": 53.20574188232422, "start": 47, "stop": 103 } ], "seq_stats": { "molecular_weight": 12189.858899999992, "isoelectric_point": 5.185955619812011 }, "id": "NKOCPLAKPK_1771", "locus": "GCA003260355_01759" }, { "type": "cds", "sequence": "contig_2", "start": 78879, "stop": 79097, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAGGAAAATATATTGTTACCCAGAGATGGGAAAATGCGAATCGTGATGGTATTGTGGGGTACATTTATCCATAAGTTACAAGTCACTTATTATATGAAGAAACACCTTGATGCGAGGTGTTTTTTCTTACAAAAAAAATCAGATTTTATCCTTGTTTTCAGAGATAGAATACGTATAAGTAAACAGCATTTTATTCTAGTTTTATTTAATCGCTAA", "aa": "MQENILLPRDGKMRIVMVLWGTFIHKLQVTYYMKKHLDARCFFLQKKSDFILVFRDRIRISKQHFILVLFNR", "aa_hexdigest": "5db85d139e49f1dc78a8cee17b9de511", "start_type": "ATG", "rbs_motif": "TTATT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8885.659299999992, "isoelectric_point": 10.495082283020018 }, "id": "NKOCPLAKPK_1772", "locus": "GCA003260355_01760" }, { "type": "cds", "sequence": "contig_2", "start": 79231, "stop": 79755, "strand": "-", "frame": 2, "gene": "lysM", "product": "LysM domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4IJM1", "UniRef:UniRef90_A0A328ECU9" ], "nt": "TTGGTTGTAAGTGACAATGAAGAAGGTATTCTTTATGTCGTAGAAAAAGAGCGAAGTCTGAAACTTTTTGCCGAGTTTTATAGACTCAACATCGACGACTTGATGACCTTCAATTACTTTTCTGATGACAGTGAAATCTTGTATCCTGGTCAGGAGTTGTTTATTAATGTGACAGAACAGAGAGCTTATGATATAGGTCTACTAGAAAAAGCTCAGCCAATATTACCAAAAGATGAAGTTGCTAAACCAAAAACAGCGAAGAAAACAGCAACTTACACAAAGGGAGCAGTTGTGTATACCAATGGTGGAAGCGCTTCAGCGGTTAATCCAGGTAATGCGAAATCTGCTATTATCAGACAATGGTATTATAATCCTAAGATTTCAAACGGATTTGCTGTAGGATATTGTACTTGGTATGCAGCGATGAAGGCTCCGGGAATCTTTAGCTATACGAGTGATACCAAGCAGGAGAGACCATTTGGAGGAAATGCGAGGGATTGGTATGCTAACGCAAAAGCCGCATGA", "aa": "MVVSDNEEGILYVVEKERSLKLFAEFYRLNIDDLMTFNYFSDDSEILYPGQELFINVTEQRAYDIGLLEKAQPILPKDEVAKPKTAKKTATYTKGAVVYTNGGSASAVNPGNAKSAIIRQWYYNPKISNGFAVGYCTWYAAMKAPGIFSYTSDTKQERPFGGNARDWYANAKAA", "aa_hexdigest": "bc0fbc64806c55e84d9ce4f576b02577", "start_type": "TTG", "rbs_motif": null, "genes": [ "lysM" ], "truncated": "both", "pseudogene": { "start": 79021, "stop": 80055, "observations": { "mutation": [ 79125, 79203, 79233, 79821, 79950, 79998 ], "stop": [ 79125, 79203, 79233, 79821, 79950, 79998 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328ECU9", "query_cov": 1.0, "subject_cov": 0.48739495798319327, "identity": 0.9940000000000001, "score": 349.0, "evalue": 3.66e-119, "gene_start": 1, "gene_end": 174, "reference_start": 114, "reference_end": 287, "reference_sequence": "MTRKTKVSKTKLLFTILSIVLFTVVLVNVDAFGSSVEDATVPVNADFLGVEEPDVGINPDNITLSDNGEESPVVSYIVQEGDSLEKISKEFGVPIKDLQRLNKIKDQPKVGQKLVVSDNEEGILYVVEKERSLKLFAEFYRLNIDDLMTFNYFSDDSEILYPGQELFINVTEQRAYDIGLLEKAQPILPKDEVAKPKTAKKTATYTKGAVVYTNGGSASAVNPGNAKSAIIRQWYYNPKISNGFAVGYCTWYAAMKAPGIFSYTSDTKQERPFGGNARDWYANAKAAGYNVGSTPRAGAIIVYRQLRSYAGHVGIVKEYHPDSGEMIIEDMNYAGKYIVTQRWENANRDGIVGYIYP" }, "paralog": false, "identity": 0.9709302325581395, "score": 662.0, "evalue": 9.25e-245, "description": "Internal stop codon at 79125, 79203, 79233, 79821, 79950, 79998. Nonsense mutation around 79125, 79203, 79233, 79821, 79950, 79998.", "psc": { "uniref90_id": "UniRef90_A0A328ECU9", "product": "LysM domain-containing protein", "uniref50_id": "UniRef50_A0A2W4IJM1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328ECU9", "UniRef:UniRef50_A0A2W4IJM1" ] }, "pscc": { "uniref50_id": "UniRef50_A0A2W4IJM1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4IJM1" ], "product": "LysM peptidoglycan-binding domain-containing protein" } }, "id": "NKOCPLAKPK_1773", "locus": "GCA003260355_01761" }, { "type": "cds", "sequence": "contig_2", "start": 79819, "stop": 79947, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAGGAATCGGATGTTGGAATAAATCCAGACAATATAACTCTTTCTGATAATGGTGAGGAATCTCCTGTGGTTTCTTATATCGTGCAGGAAGGTGATAGTTTAGAAAAGATATCAAAAGAATTTTGA", "aa": "MEESDVGINPDNITLSDNGEESPVVSYIVQEGDSLEKISKEF", "aa_hexdigest": "a78e1cb8d87987b32a087ef7abf25b05", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4614.909099999999, "isoelectric_point": 4.0500284194946286 }, "id": "NKOCPLAKPK_1774", "locus": "GCA003260355_01762" }, { "type": "tRNA", "sequence": "contig_2", "start": 80114, "stop": 80203, "strand": "-", "gene": "trnS", "product": "tRNA-Ser(tga)", "amino_acid": "Ser", "anti_codon": "tga", "score": 77.8, "nt": "GGAGGGATGGCAGAGTGGTTGAACGCGGCAGTCTTGAAAACTGTTAACCGATTATTCGGTTCGTGGGTTCAAATCCCACTCCCTCCGCCA", "db_xrefs": [ "SO:0000269" ], "anti_codon_pos": [ 80182, 80184 ], "id": "NKOCPLAKPK_32", "locus": "GCA003260355_01763" }, { "type": "cds", "sequence": "contig_2", "start": 80567, "stop": 80716, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01327.26" ], "nt": "ATGATTGCTACTACCCAGTGGAAAAAGGTCCCACAAGGAAAAAATGCAGACAAAGATTATTTGGGAGAAACCTTACTCATTAATCCTGAAATTGTCGACAAATCGAAGGAAATGCAAATTTCTGAAGAAGCCTGTCTTTCTCTTCCATGA", "aa": "MIATTQWKKVPQGKNADKDYLGETLLINPEIVDKSKEMQISEEACLSLP", "aa_hexdigest": "c0498e9ee1d3e124c4bca3928baeacf1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01327.26", "name": "Pep_deformylase", "length": 36, "aa_cov": 0.7346938775510204, "hmm_cov": 0.9166666666666666, "evalue": 1.1402032711161321e-07, "score": 27.983211517333984, "start": 14, "stop": 49 } ], "seq_stats": { "molecular_weight": 5505.298, "isoelectric_point": 4.812637138366699 }, "id": "NKOCPLAKPK_1775", "locus": "GCA003260355_01764" }, { "type": "cds", "sequence": "contig_2", "start": 81042, "stop": 81293, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATCGAGGGATATGAAATCTTTTTGCCAAGTATGCGTAGTTATGGAGGAAATATCGAACAAAAAGATATTGATATGGTGATTGAAAAAATCAAACAAGATGTGCGCTTTCAAAGTACCTTTCTAGTAGATCAAGATCCTGTAAAAAATAAACCACTTCCTTCTTCTTCATTTTCTCCAACAGCAGAAAGCTGGAAGTGTGAAACCTGCACCTTCAGAAAAGTTTGCCATCAACTAAGGGCGCTTGAATAG", "aa": "MIEGYEIFLPSMRSYGGNIEQKDIDMVIEKIKQDVRFQSTFLVDQDPVKNKPLPSSSFSPTAESWKCETCTFRKVCHQLRALE", "aa_hexdigest": "93948aa85c2aceee141ad55421a6b816", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9616.939999999999, "isoelectric_point": 5.805159568786621 }, "id": "NKOCPLAKPK_1776", "locus": "GCA003260355_01765" }, { "type": "cds", "sequence": "contig_2", "start": 81441, "stop": 81566, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGAAATTCTAATGCAATATACATTGAAAATCCAAAAAATCCTGACTTTGAGGCTATGAAAGTAGAGATTGATAAAATCCAGGATCTCAAAGACATTTCGATTATGGCGAGTCCGGATTTTTGA", "aa": "MRNSNAIYIENPKNPDFEAMKVEIDKIQDLKDISIMASPDF", "aa_hexdigest": "04115da947f73fd753edb736840d100b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4742.362999999999, "isoelectric_point": 4.4576208114624025 }, "id": "NKOCPLAKPK_1777", "locus": "GCA003260355_01766" }, { "type": "cds", "sequence": "contig_2", "start": 81567, "stop": 82097, "strand": "-", "frame": 3, "gene": null, "product": "PD-(D/E)XK endonuclease-like domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MWI1", "UniRef:UniRef90_A0A328EGD0" ], "nt": "ATGATAAAACCAAAGCATCTGATTTTAAATGCTTTTCTAAAAAAGATGATCTACTCTTCTCACAAAAAACCGAAGATGAATAAGCTTAGTTGGTCAAACACGAGGATTACACTCTTAGAATACTGTGAAAAGAAATATTATCTCAACTATTACACTTTTGCACTCAAAAACTTTAATGAAGAACTCCGAAGAAATACCCTCATTTCAAAAAAACTCAAATCTCTGGAAATGTGGATGGGTGAGAAGACGCATCTTTTGATTTCCGATTATTTAAAACTCTTACAAGCAGGAGAGGCTACTGCAGAAAATATCCAAAAAATTAAGGAAGGAATTGCTGAGGAGATGAGATATGAATTTGAGCTTTCAAAGGAAAAAGATTATGAAACACTGAATTTTGAATCTTGGGGAGGATTAAGTGAGCATTTTTACCACGAAAATATTGATGAGAGATTGAATCCGACGATTCAGAGAGTATTTGCTAACTTAGATGCTTTGATTTCCTCTCCATGGATTGAGAGAATCCAGGAATGA", "aa": "MIKPKHLILNAFLKKMIYSSHKKPKMNKLSWSNTRITLLEYCEKKYYLNYYTFALKNFNEELRRNTLISKKLKSLEMWMGEKTHLLISDYLKLLQAGEATAENIQKIKEGIAEEMRYEFELSKEKDYETLNFESWGGLSEHFYHENIDERLNPTIQRVFANLDALISSPWIERIQE", "aa_hexdigest": "b45e87e6ed2822eb900fb6cf661935e3", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 81264, "stop": 82097, "observations": { "mutation": [ 81302, 81431, 81443, 81569 ], "stop": [ 81302, 81431, 81443, 81569 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EGD0", "query_cov": 1.0, "subject_cov": 0.5014245014245015, "identity": 0.972, "score": 338.0, "evalue": 1.18e-114, "gene_start": 1, "gene_end": 176, "reference_start": 1, "reference_end": 176, "reference_sequence": "MIKPKHLILNAFLKKMIYSSHKKSKMNKLSRSNTRITLLEYCEKKYYLNYYTFALKNFNEELRRNTLISKKLKSLEMWMGEKTHLLISDYLKLLQAGEATAENIQKIKEGIAEEMRYEFELSKEKDYETLNFESWGGLSEHFYHENVDERLDPTIQRVFANLDALITSPWIERIQEGMRNSNAIYIENPKNPDFEAMKVEIDKIQDLKDISIMASPDFGIIFGGNRYLILDRKSGKEPQYPIDIADQIKVYALKLLLKKKKMPVLGDIMIEGYEIFLPSMHSYGGNIEQKDIDMVIEKIKQDVRFQSTFLVDQDPVKNKPLPSSSFSPTAESWKCETCTFRKVCHQLRALE" }, "paralog": false, "identity": 0.9675090252707581, "score": 530.0, "evalue": 6.78e-193, "description": "Internal stop codon at 81302, 81431, 81443, 81569. Nonsense mutation around 81302, 81431, 81443, 81569.", "psc": { "uniref90_id": "UniRef90_A0A328EGD0", "product": "PD-(D/E)XK endonuclease-like domain-containing protein", "uniref50_id": "UniRef50_A0A660MWI1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EGD0", "UniRef:UniRef50_A0A660MWI1" ] }, "pscc": { "uniref50_id": "UniRef50_A0A660MWI1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MWI1" ], "product": "PD-(D/E)XK endonuclease-like domain-containing protein" } }, "id": "NKOCPLAKPK_1778", "locus": "GCA003260355_01767" }, { "type": "cds", "sequence": "contig_2", "start": 82157, "stop": 82300, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGCGGAAATGCAGAAAGTCGTGCTCCTGCCTGATCTCGCCATTCAGGGATATTTTGAAGTAGTGTATCCATATATCCTCACATCGTTTACCTTTTCAATGAACTGTGATAAAAATTCACCTCCCAAATTAGTGATTTAA", "aa": "MKAEMQKVVLLPDLAIQGYFEVVYPYILTSFTFSMNCDKNSPPKLVI", "aa_hexdigest": "62827969f27709da89b12f0dfa7311b1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5383.3662, "isoelectric_point": 5.967946434020996 }, "id": "NKOCPLAKPK_1779", "locus": "GCA003260355_01768" }, { "type": "cds", "sequence": "contig_2", "start": 82315, "stop": 82473, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTCTCCTATAATTTCTATAAAGAAATCATAGATCTTTTCAAACATCATCTCAAACAAGAGGATGAAGCTATTTCAAAACTTCGTAAAAATCTGTTTTCCAGACTTAAACCTATGATAGATAATGACAAAAACAATAATCAAAAAAAGGAGGATTAA", "aa": "MFSYNFYKEIIDLFKHHLKQEDEAISKLRKNLFSRLKPMIDNDKNNNQKKED", "aa_hexdigest": "be557b6ecb2f3d2e95e9b85fa330b388", "start_type": "ATG", "rbs_motif": "TTTA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6416.260000000001, "isoelectric_point": 9.004702186584474 }, "id": "NKOCPLAKPK_1780", "locus": "GCA003260355_01769" }, { "type": "cds", "sequence": "contig_2", "start": 82507, "stop": 82683, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTTTTGTGATTCTGTTTTCAAATCTTATGGGAACACTCCTTGACTTCGTAGGGATTGCGTTTCCTGGTTTGGAAGAGTGGATTATTGCTCCTACAACCGATGCTAACTTTAATATTGCGATGGCGGTGGTGTCAGTTCTTCTGATTCTTTATCTTCAGATGAAACACTTGTGA", "aa": "MFFVILFSNLMGTLLDFVGIAFPGLEEWIIAPTTDANFNIAMAVVSVLLILYLQMKHL", "aa_hexdigest": "7624befb9ef26124f8b76521a3962613", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6503.793299999999, "isoelectric_point": 4.307679557800294 }, "id": "NKOCPLAKPK_1781", "locus": "GCA003260355_01770" }, { "type": "cds", "sequence": "contig_2", "start": 82753, "stop": 83082, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00119.25" ], "nt": "ATGCCAAAACTCGTATATTATCCGCTGTGGCTGTTTGCTAAACTCTTTGATATTCTGATTTCCCTTTTTGTAGGGGCGCTTGATATTATTGGAAACTTTGCTAAAGTAATTTCGCTTTCCTTCAGATTAACAGGTAATATGATGTCGGGAACGATTTTGCTAGGGATGCTTGTGATGGGACTCAAAGCTTTGACGAGTTCAATGACGGGATTCGAGTTTCCAATCCTCTTCCCTTTGATCATCGTATTGCAAGGGGTCTTGGTTGCGATCATCCAAGCTTTTGTATTTGCACTTTTGACGGCAATCTTTATAAAGGTTGCTACTGAATAG", "aa": "MPKLVYYPLWLFAKLFDILISLFVGALDIIGNFAKVISLSFRLTGNMMSGTILLGMLVMGLKALTSSMTGFEFPILFPLIIVLQGVLVAIIQAFVFALLTAIFIKVATE", "aa_hexdigest": "199482e6109ae3c3970091c73cf0b217", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00119.25", "name": "ATP-synt_A", "length": 96, "aa_cov": 0.8440366972477065, "hmm_cov": 0.9895833333333334, "evalue": 4.810170908000682e-19, "score": 65.5146713256836, "start": 14, "stop": 105 } ], "seq_stats": { "molecular_weight": 11946.612299999986, "isoelectric_point": 9.22402362823486 }, "id": "NKOCPLAKPK_1782", "locus": "GCA003260355_01771" }, { "type": "cds", "sequence": "contig_2", "start": 83250, "stop": 83660, "strand": "+", "frame": 3, "gene": "atpE", "product": "F0F1 ATP synthase subunit C", "db_xrefs": [ "EC:7.1.2.2", "GO:0015078", "GO:0015986", "SO:0001217", "UniRef:UniRef50_A0A2W4IIQ4", "UniRef:UniRef90_A0A2W4IIQ4" ], "nt": "TTGGCAGGTATTGGTGCTGGACTTGGAGAAGCGATGATCGTTGCTGCGGCACTTAATGCGATTTTAAGAAATCCAGAAATGAAAGGAAAACTGATGACTTTCATGATTCTCTTTATTGCGCTGGATGAATCAGTAGCGATCTATGGATTGATCGTAGCGTTTAAGATTTTGGCGGTAGATCCTACTGCGATGACGAATCCTTATATGTATGTTGCAGCAGGGCTTGCTATTGGGCTTCCTGGACTTGCGGTTGGAATTGGTGAAGGATATTTGGCGAAGGTTTCTATTGATAATATGGGAAAGAATCCTGAACTCAGTTCAACTTTTATGGTGCTTACCATTCTTGGACTTGCACTTGTGGAATCAGCTGCGATTTACGGATTGATTGTAGCGTTTAGATTGATTGGATAA", "aa": "MAGIGAGLGEAMIVAAALNAILRNPEMKGKLMTFMILFIALDESVAIYGLIVAFKILAVDPTAMTNPYMYVAAGLAIGLPGLAVGIGEGYLAKVSIDNMGKNPELSSTFMVLTILGLALVESAAIYGLIVAFRLIG", "aa_hexdigest": "1d98c77a78473cdd6fdbd2d2f40af67e", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A2W4IIQ4", "query_cov": 1.0, "subject_cov": 0.9066666666666666, "identity": 0.963, "score": 237.0, "evalue": 1.42e-78, "valid": true, "gene": "atpE", "product": "F0F1 ATP synthase subunit C", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_A0A2W4IIQ4", "go_ids": [ "GO:0015078", "GO:0015986" ] }, "pscc": { "uniref50_id": "UniRef50_A0A2W4IIQ4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4IIQ4" ], "product": "ATP synthase subunit c" }, "genes": [ "atpE" ], "id": "NKOCPLAKPK_1783", "locus": "GCA003260355_01772" }, { "type": "cds", "sequence": "contig_2", "start": 83712, "stop": 84164, "strand": "+", "frame": 3, "gene": null, "product": "ATP synthase subunit b", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7W4EBS5", "UniRef:UniRef90_A0A328ECT9" ], "nt": "GTGATTGCGGGGATCGTCAACTTTCTTGTGCTGGTGTTCCTTTTTAGGAAGCTTCTGGGAGACAAGATCGTCCAAGAAGTAGAAAAGAGGAGGACCATGCTTGAAAAACTAAAGAAAGCTGATGATGAATACAAGAAGATGATTGAATTTGCTCGTAAAGAGTCAGATTTGATTCTTGAAAAAGCAGAAAAGACAAAAAAAGATATGATTCATGAAGCACACTTAGTAGCCGAAGAGGAGAAGTCCAGGATTCTCAAGCAAGCTCATCACAAAGCGGACTTGATCGTCAGTGAAGCAAAGCAAGACACGGATAAATTGGAGAGAGAGCTGAAAGAAAGTTGGGTTGCTTCGGTAAAAGAAACCTCCAAAAAGGTCGTAAAAAGACTCCTCAAGCATGATAAGGTGCTGGAAGATGAATACTTTGGAGTTTTGGTAGAAGATTTGAAAGATTAG", "aa": "MIAGIVNFLVLVFLFRKLLGDKIVQEVEKRRTMLEKLKKADDEYKKMIEFARKESDLILEKAEKTKKDMIHEAHLVAEEEKSRILKQAHHKADLIVSEAKQDTDKLERELKESWVASVKETSKKVVKRLLKHDKVLEDEYFGVLVEDLKD", "aa_hexdigest": "8e27e2657297672c050410a21e1f3720", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A328ECT9", "query_cov": 0.9933333333333333, "subject_cov": 0.9490445859872612, "identity": 0.966, "score": 256.0, "evalue": 7.57e-86, "valid": true, "product": "ATP synthase subunit b", "uniref50_id": "UniRef50_A0A7W4EBS5" }, "pscc": { "uniref50_id": "UniRef50_A0A7W4EBS5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7W4EBS5" ], "product": "ATP synthase subunit b" }, "genes": [], "id": "NKOCPLAKPK_1784", "locus": "GCA003260355_01773" }, { "type": "cds", "sequence": "contig_2", "start": 84310, "stop": 84636, "strand": "+", "frame": 1, "gene": null, "product": "Peptidase C51 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBFBCF6", "UniRef:UniRef100_A0A328EG84", "UniRef:UniRef50_A0A328EG84", "UniRef:UniRef90_A0A328EG84" ], "nt": "TTGTGCTTTAGTGATACAAAAAATTTCCCAGATCTAGCTCGCTATAAAAATGGTGGCTCTGTTGCCCCGAGACCTGGAGATATTTTGTGTCTATCTGGAGGGGAGGGAAATGGCGGGCATGTTGCCATTATCATGGAAGTGACCAAGACTTACATTAAAATTGCACACCAGAACTCTGGAGATCGATGGGATGCAATTGGAGCAAGTCTTGATATGAAAGATACGAAAGTTGCCAATCCTTCAGGATATACAGTTCAAGGATGGTTGAGAATTCCTACCTATCTTAATGACCTTCCTAATCCTCCAATTCCAGAGTATCTCAAGTAA", "aa": "MCFSDTKNFPDLARYKNGGSVAPRPGDILCLSGGEGNGGHVAIIMEVTKTYIKIAHQNSGDRWDAIGASLDMKDTKVANPSGYTVQGWLRIPTYLNDLPNPPIPEYLK", "aa_hexdigest": "8781f5ffafc00e69ffead36018dc0883", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000DBFBCF6", "uniref100_id": "UniRef100_A0A328EG84", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBFBCF6", "UniRef:UniRef100_A0A328EG84" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EG84", "uniref90_id": "UniRef90_A0A328EG84" }, "psc": { "uniref90_id": "UniRef90_A0A328EG84", "product": "Peptidase C51 domain-containing protein", "uniref50_id": "UniRef50_A0A328EG84" }, "pscc": { "uniref50_id": "UniRef50_A0A328EG84", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EG84" ], "product": "Peptidase C51 domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_1785", "locus": "GCA003260355_01774" }, { "type": "cds", "sequence": "contig_2", "start": 84740, "stop": 84928, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACCCGAGAGCTTATACTATTTTTAGACTTTTTATTAAGTCAAAGTCTGAATATGAAAAATATAGCGATCGAAGTTATTATGATTTTATTGGGTGTAATAATAAATATTGCTTTTTGGGAGGTAAGGATCCAGGTATGAATAATTATTATACCAAACGTTACTCAAAGGTAAAGAGAGAAAGTTGA", "aa": "MNPRAYTIFRLFIKSKSEYEKYSDRSYYDFIGCNNKYCFLGGKDPGMNNYYTKRYSKVKRES", "aa_hexdigest": "220d5497e142a53eb492b3e068077a0a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7536.475300000003, "isoelectric_point": 9.546752357482909 }, "id": "NKOCPLAKPK_1786", "locus": "GCA003260355_01775" }, { "type": "cds", "sequence": "contig_2", "start": 85040, "stop": 85210, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCAAGAGGCGGGAACTCTGAAAGAGGAACAAGAAGATGCTTGGATACCGAAAGGCTATACCGGAGCGATAGCCTTGATTACCGAAGCGAATAGCGGCGATGTTATCACCCAAACTGGCGATCAACTTTTGTATAGGAATGAAGTGTATGGGTTTCAGGTTTTGCTATGA", "aa": "MQEAGTLKEEQEDAWIPKGYTGAIALITEANSGDVITQTGDQLLYRNEVYGFQVLL", "aa_hexdigest": "03a4433dc9bfa3b4be2e4d9e129b39b4", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6189.820600000001, "isoelectric_point": 4.056167030334472 }, "id": "NKOCPLAKPK_1787", "locus": "GCA003260355_01776" }, { "type": "cds", "sequence": "contig_2", "start": 85545, "stop": 86438, "strand": "+", "frame": 3, "gene": null, "product": "Peptidase C51 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EC55", "UniRef:UniRef90_A0A328EC55" ], "nt": "ATGGAAAGAAAAAAAATTGCACTGCTTGCAGTGCTCGTGTGTATTCTTGTCTCAGGAAGTCTTACTTCTTGTGATAAAGATGAAAGAACGATCCCTTCTCATCAAGAGGAGGGGATTGCATCCAAAAGTGAGGGCAATGATCTTCGTTCTGAGAGTCTAAGAGCACGATTGGATGTCGTTCCATCTCAGGCTTTACGTGGAACTAATTTCCGGTTCGTCGTTTGGATATCGGGACGAAATCCTCAGAACAAAAAAGCGGCATTGCGTTTTGAATGTCCTGATGGAGTTACTCGAACCTTTGATATGCAAACGAATGGATATGATGTTGAAATGGCCTTTTTTGTACTGAACCGTTCTCTATCGCAATACGGTGAATACAGAGTATCGGCATATGAAAAGGTAGGTAATGGTTATTATAGCCTCTTCTGTAGCGAACGTGTCTTCGTATTACATCCTCCTATCCCTATGGGTGATAACTATTCATCACTTCATGTAGGAAAAGGTGTAATGTACCCTAGGAATTGTACTGCTTGGGTAACTGGCAAGGTAAACGAAATGTGGAATGCTAACTCTTTTCAGAACCTTATAGACAACCCAAGAGATGCGAAACATTGGAAGAATCACCTTGTTGCCAAAGGATATAAAGCCGATGGTAATCCTCGTGTTGGTGATATTGCTTTCTGGGAAGCAAATATCAAGGGATGTTCTGGAAGTCTTGGACACGTTGCTTTTGTCAACAATGTCTCTTCGGATGGGAATACTGTTTATATTACAGAATACAATTTCCCTTCAGGAGAACGATACGGGACTCGAACCCTAAAGAGGTATGGAGCGAGAAAGCCAAGTGAACAATTCCCTACAGCCTTCATTCACGTGCAAACAAGAAGAAATTAA", "aa": "MERKKIALLAVLVCILVSGSLTSCDKDERTIPSHQEEGIASKSEGNDLRSESLRARLDVVPSQALRGTNFRFVVWISGRNPQNKKAALRFECPDGVTRTFDMQTNGYDVEMAFFVLNRSLSQYGEYRVSAYEKVGNGYYSLFCSERVFVLHPPIPMGDNYSSLHVGKGVMYPRNCTAWVTGKVNEMWNANSFQNLIDNPRDAKHWKNHLVAKGYKADGNPRVGDIAFWEANIKGCSGSLGHVAFVNNVSSDGNTVYITEYNFPSGERYGTRTLKRYGARKPSEQFPTAFIHVQTRRN", "aa_hexdigest": "a396ee2db0ace32abeaea9c625ae4d44", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A328EC55", "query_cov": 1.0, "subject_cov": 0.9054878048780488, "identity": 1.0, "score": 611.0, "evalue": 5.6e-221, "valid": true, "product": "Peptidase C51 domain-containing protein", "uniref50_id": "UniRef50_A0A328EC55" }, "pscc": { "uniref50_id": "UniRef50_A0A328EC55", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EC55" ], "product": "Peptidase C51 domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_1788", "locus": "GCA003260355_01777" }, { "type": "cds", "sequence": "contig_2", "start": 86749, "stop": 87195, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI000CB295E7", "UniRef:UniRef100_A0A2N8NGB0", "UniRef:UniRef50_A0A2N8NGB0", "UniRef:UniRef90_A0A2N8NGB0" ], "nt": "ATGGATAGTCTGCAACTTCTTCCGAAGTTCCCTCAGACGGGTGCTTTTCTGAAATTTTATGCAAATAAAGATTGGAATTTTTCCCTCTTAGCACCAAAAAACGTTGCTCCACATCAGGAAAGCTTGAGGAAAATCCCCTCTCCCTATGATCGATTTGACCTTTGGGAAGATGAAGAGTATTACTATCTGTATTCAAATAATTCTCAGGAGTATAGATGGTTTGCCAACAAAAAATCTGCATTGCCGAACTTCAAACTTCTCGGTTTCCTCGGGGAGCACGCTATTGATGCGTTTTTTGGCTATAGTGATGGGTATATCGTCATCCCTCCTTACCTCTTGCCTGTGGATCACAGCACGTATATTCCTCCCCAGACGCCTGGCAGTGGAGGAATCCAGGATCGACAGTTCTTTAGAAACGGGGGAAAGCAATACACCAACTATTTATGA", "aa": "MDSLQLLPKFPQTGAFLKFYANKDWNFSLLAPKNVAPHQESLRKIPSPYDRFDLWEDEEYYYLYSNNSQEYRWFANKKSALPNFKLLGFLGEHAIDAFFGYSDGYIVIPPYLLPVDHSTYIPPQTPGSGGIQDRQFFRNGGKQYTNYL", "aa_hexdigest": "a2e3c877a9e90c5707c1e725fe006ec7", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000CB295E7", "uniref100_id": "UniRef100_A0A2N8NGB0", "db_xrefs": [ "SO:0001217", "UniParc:UPI000CB295E7", "UniRef:UniRef100_A0A2N8NGB0" ] }, "ips": { "uniref100_id": "UniRef100_A0A2N8NGB0", "uniref90_id": "UniRef90_A0A2N8NGB0" }, "psc": { "uniref90_id": "UniRef90_A0A2N8NGB0", "uniref50_id": "UniRef50_A0A2N8NGB0" }, "pscc": { "uniref50_id": "UniRef50_A0A2N8NGB0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2N8NGB0" ] }, "genes": [], "truncated": "both", "pseudogene": { "start": 86524, "stop": 87498, "observations": { "mutation": [ 86575, 86611, 86653, 87193, 87337, 87373, 87445 ], "stop": [ 86575, 86611, 86653, 87193, 87337, 87373, 87445 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A2N8NGB0", "query_cov": 1.0, "subject_cov": 0.40437158469945356, "identity": 1.0, "score": 316.0, "evalue": 1.95e-106, "gene_start": 1, "gene_end": 148, "reference_start": 76, "reference_end": 223, "reference_sequence": "MSIKRYRNLFLLSLIVCGGLFFSDFFGKPGAPSALPQYEEPFTGGISTGFLEEPRAPLGTPEDYARRIFVDAPVAMDSLQLLPKFPQTGAFLKFYANKDWNFSLLAPKNVAPHQESLRKIPSPYDRFDLWEDEEYYYLYSNNSQEYRWFANKKSALPNFKLLGFLGEHAIDAFFGYSDGYIVIPPYLLPVDHSTYIPPQTPGSGGIQDRQFFRNGGKQYTNYLGSDKDWVYRWNKDNSFENYLLLREKRKSDFKLLTGEEIQEFFNLSHIPGVQLHPKNQEKRGTQLTDGYRKIPRLRHFGDRMYVFGVHGNWSFPIDGASMQRTEKEIPNNENHTTYFTLSDNNYFYQLVYHNEGDFYELQRIKK" }, "paralog": false, "identity": 0.9783950617283951, "score": 658.0, "evalue": 1.52e-243, "description": "Internal stop codon at 86575, 86611, 86653, 87193, 87337, 87373, 87445. Nonsense mutation around 86575, 86611, 86653, 87193, 87337, 87373, 87445.", "psc": { "uniref90_id": "UniRef90_A0A2N8NGB0", "uniref50_id": "UniRef50_A0A2N8NGB0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A2N8NGB0", "UniRef:UniRef50_A0A2N8NGB0" ] }, "pscc": { "uniref50_id": "UniRef50_A0A2N8NGB0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2N8NGB0" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 17267.185499999978, "isoelectric_point": 6.27055721282959 }, "id": "NKOCPLAKPK_1789", "locus": "GCA003260355_01778" }, { "type": "cds", "sequence": "contig_2", "start": 87250, "stop": 87663, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAATATTCTAACACTTGTGAAAGAATCGCACGTTCTCTACTTTTTAATCCTCTGAAGCTCATAGAAATCTCCCTCATTATGATACACGAGCTGATAAAAATAGTTGTTATCAGACAATGTAAAGTAAGTAGTATGATTTTCATTATTAGGAATTTCTTTTTCTGTTCGCTGCATACTTGCCCCATCGATCGGAAAGCTCCAATTTCCATGCACTCAGAAAACATACATTCTATCCCCGAAATGCCGAAGTCTAGGAATCTTCCGATAGCCATCTGTCAACTGAGTTCAGCGTTTTTCTTGGTTTTTCGGATGAAGTTGCACTCACGGGATGTGAGAAAGGTTGAAAAATTCTTGGATTTCCTCTCCAGTCAACAGTTTAAAGTCAGACTTTCTCTTTTCTCTGAGAAGTAA", "aa": "MEYSNTCERIARSLLFNPLKLIEISLIMIHELIKIVVIRQCKVSSMIFIIRNFFFCSLHTCPIDRKAPISMHSENIHSIPEMPKSRNLPIAICQLSSAFFLVFRMKLHSRDVRKVEKFLDFLSSQQFKVRLSLFSEK", "aa_hexdigest": "2f7c15b9d19ebc6765971e2625b68899", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16105.129999999983, "isoelectric_point": 9.715272712707517 }, "id": "NKOCPLAKPK_1790", "locus": "GCA003260355_01779" }, { "type": "cds", "sequence": "contig_2", "start": 87934, "stop": 88053, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTATAGGCTTGGGACGAGCAGAGAAAGTGAAAGTTATAATGAGATTACAGAACGTTCTGCACGGGTCGTCCATGACCTAGTGGGGGACAAAATGATTGTAAAAAGGCTGACGAACTGA", "aa": "MYRLGTSRESESYNEITERSARVVHDLVGDKMIVKRLTN", "aa_hexdigest": "9e1dcefe1c08f98d7489d428efdcdfc7", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4555.115499999999, "isoelectric_point": 8.210516166687015 }, "id": "NKOCPLAKPK_1791", "locus": "GCA003260355_01780" }, { "type": "cds", "sequence": "contig_2", "start": 88093, "stop": 88416, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2N8NGB8", "UniRef:UniRef90_A0A2N8NGB8" ], "nt": "TTGTGGAAGAATTTCATTACAGGTCCCGTATGGCACGAACTTTCTTCTGAGAGGGGGAAAAGTCTTTGTGTGTTCAATGGTGATGAATACCAAGAACTCACCTCTCCAAGAGAAAAGGAAGGTGAAGTCTTCATCAGAAAACATGACTTACAAGATAATCCGTATTTCAGCTTTATTTACCCTTATATCGGGGATGAGGATGAAATCGTGATCCAACAGATGACAAAGTGTGAAGAAATTGAGCTTTGTAGAGATTTAGTCCCAAAAGTTCGAGGAGATAATGAACATCATTTCTTCTTTCATACGCAGGCTTCGATGACGTGA", "aa": "MWKNFITGPVWHELSSERGKSLCVFNGDEYQELTSPREKEGEVFIRKHDLQDNPYFSFIYPYIGDEDEIVIQQMTKCEEIELCRDLVPKVRGDNEHHFFFHTQASMT", "aa_hexdigest": "9c9472cb489b8f9fe8b7109170b170b7", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 88093, "stop": 88502, "observations": { "deletion": [ 88442 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A2N8NGB8", "query_cov": 1.0, "subject_cov": 0.7867647058823529, "identity": 0.991, "score": 231.0, "evalue": 5.21e-77, "gene_start": 1, "gene_end": 107, "reference_start": 30, "reference_end": 136, "reference_sequence": "MLQGIVSIRKDISFMLLFEEGGGGRSPLYLWKNFITGPVWHELSSERGKSLCVFNGDEYQELTSPREKEGEVFIRKHDLQDNPYFSFIYPYIGDEDEIVIQQMTKCEEIELCRDLVPKVRGDNEHHFFFHTQASMT" }, "paralog": false, "identity": 1.0, "score": 282.0, "evalue": 3.02e-99, "description": "Frameshift due to deletion around 88442.", "psc": { "uniref90_id": "UniRef90_A0A2N8NGB8", "uniref50_id": "UniRef50_A0A2N8NGB8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A2N8NGB8", "UniRef:UniRef50_A0A2N8NGB8" ] }, "pscc": { "uniref50_id": "UniRef50_A0A2N8NGB8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2N8NGB8" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 12733.12379999999, "isoelectric_point": 4.89079074859619 }, "id": "NKOCPLAKPK_1792", "locus": "GCA003260355_01781" }, { "type": "cds", "sequence": "contig_2", "start": 88447, "stop": 88746, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGGAAAGGAGTAGTTTTATTAGGGATGCTTTCAAGTGCTTTGGCAGTGATGGGATGTTCATTTTCTCAACAGCCAGTTCCTCAAGAGGAAATGCTCTCTCATTCAGGAAAACAGCTTACTCAAGCATTCACCGGAGGAACCTGTGATACACCAAACCTTGCGGAATTGCAAAAGCCGATTCCTCCCTGTGAACCCTTAGATATCAAGCCTTTCCCTGAAGCGGACATCAAAAATATAGAGGATGTTGCAAGGAATTGTAAGTATCAGAAAGGACATTTCTTTTATGCTCCTTTTTGA", "aa": "MRKGVVLLGMLSSALAVMGCSFSQQPVPQEEMLSHSGKQLTQAFTGGTCDTPNLAELQKPIPPCEPLDIKPFPEADIKNIEDVARNCKYQKGHFFYAPF", "aa_hexdigest": "2281332b5f60c3e5417b1cc3256d9044", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10884.501599999987, "isoelectric_point": 6.051670265197754 }, "id": "NKOCPLAKPK_1793", "locus": "GCA003260355_01782" }, { "type": "cds", "sequence": "contig_2", "start": 88885, "stop": 90216, "strand": "-", "frame": 2, "gene": null, "product": "Putative amidase domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EBK6", "UniRef:UniRef90_A0A328EBK6" ], "nt": "TTGACATTTGCTTTAAACGCTTTTCGTGTTCCGTCTGTAACCTCAGAGCAATCTAGAGGAACAGGAGAACTTTTAGCTTGGGAGAAGGCGAGGCACGATTACTCATACCTTGAAGGAAAGGTAGAAGAGGCAAATCAATACCTTACCAAGAACAAGCTAGAACTCCAAGATCTGAAACAGTTGCATGAGATAGGAAATCTTATCTTTGGAGATTATCTTTCTAAAATTATGATGGATTTTCAGAAGAGGAGCTTAGCGTGGCTTGATGATAAGCTTTTTATCAATGAGTATGCAAAAAATAACATGTATGCTTTTTTGATTATGGATTTTCATGATGATAAGAGGTATTCTAAAGAGGTGTTGCAATCTTCTCCAGATATTCATATCATGACAAATCACATTGGCGAGAGAAATGGATGGGTCTTTATACCCATTAGAGTGTATTTCCCTGCTCCAGCCGAAGTTGCAGATGATCCCACATTTGATGAAAATGGTGGAAATGATACGAGATGGAGGTATTATGTGCTCCAACTCAAGAGAGACGAGAAATCACCTTATCATTGGAAGGTTGCTTATGAGGAACAAGTCGTAAGTCGTGGGATAGACGGAGAAATTCTGCGAGGTGCATGGTTTGCTACTCTGAATCCACAAGATGACTTTTCTGCTAAAATGTGGAAAGACGTTCTTTCAAGAAAGGATTATTGGGAATACTACATTGGAGAAAGAGACTGTCCTCGGACAGGAAGTATTCTCTGTCCTAAAAAAGAACCACTATCTCAATTAAGAAGTGGATTGTATGATAGACAAGCAGCAGCGCGCTATGCGTATTATCATGCATTATCTTATAATACTGAGTATGCAAAGTTTTCTCCTAATGATTGTGCTAATTTTGTTTCTCAGTGTCTCTATGCCGGAGGAATCCCCAAAGATAATACTTGGGGAACTCTTGATGGACGCTATCCTTATTATGGGACATCTACTTGGCTGGGAGCAAATGCTTTATACGAGTATATGAAGAAACATTATGGATATATTGTTTCTAAAGAGGATCTTGTGGCAGGATATGATGGGGACAGAGATTGTGTTCTAGAATGGGGCGACATGCTTGCAATGAATAGAAAAGGTGGAGTTGATATTGATGATGCTGTTAGAAAGACACATGTAATGATTATCTCCCATTCATATCATTACACAAATCAGCATTATAATGGAGTTCCTAGGTATAAGGTCTGCGGTCATACTAATAATAGATTGGATCTTGATATAATGTCACTTCCTGGAGGAAGGGAGTACGTGAAAAAACACATTATGGGCATCCATATTACCTACTGA", "aa": "MTFALNAFRVPSVTSEQSRGTGELLAWEKARHDYSYLEGKVEEANQYLTKNKLELQDLKQLHEIGNLIFGDYLSKIMMDFQKRSLAWLDDKLFINEYAKNNMYAFLIMDFHDDKRYSKEVLQSSPDIHIMTNHIGERNGWVFIPIRVYFPAPAEVADDPTFDENGGNDTRWRYYVLQLKRDEKSPYHWKVAYEEQVVSRGIDGEILRGAWFATLNPQDDFSAKMWKDVLSRKDYWEYYIGERDCPRTGSILCPKKEPLSQLRSGLYDRQAAARYAYYHALSYNTEYAKFSPNDCANFVSQCLYAGGIPKDNTWGTLDGRYPYYGTSTWLGANALYEYMKKHYGYIVSKEDLVAGYDGDRDCVLEWGDMLAMNRKGGVDIDDAVRKTHVMIISHSYHYTNQHYNGVPRYKVCGHTNNRLDLDIMSLPGGREYVKKHIMGIHITY", "aa_hexdigest": "9aa2d1446094c620d53f32243cf2a9df", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A328EBK6", "query_cov": 1.0, "subject_cov": 0.9910514541387024, "identity": 1.0, "score": 938.0, "evalue": 0.0, "valid": true, "product": "Putative amidase domain-containing protein", "uniref50_id": "UniRef50_A0A328EBK6" }, "pscc": { "uniref50_id": "UniRef50_A0A328EBK6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EBK6" ], "product": "Putative amidase domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_1794", "locus": "GCA003260355_01783" }, { "type": "cds", "sequence": "contig_2", "start": 90221, "stop": 90826, "strand": "+", "frame": 2, "gene": null, "product": "DUF6261 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBF8436", "UniRef:UniRef100_A0A328ECY6", "UniRef:UniRef50_A0A328ECY6", "UniRef:UniRef90_A0A328ECY6" ], "nt": "ATGATTGACGCGAATGGGCAACTGAAAAAAGATCCCAATTCTGAGGAGATCAACAAGTTGGACCAAGAACGTGATCAGCTTTTAACTTATTTTTTTACTAAGATTGCTGCAGAAATGCGTTCCCCTGATCAAGCGTTGGAAGCGGCAGCAACTGCATTGGGGGATTTTTTGGTAGTGTATCGTGGGATTCAAGGGAAAGCGAACCGTCAGGAAACGCAGCTGATCAAGAGTTTACTTTTGGATGCTGAGCAGGCGAAGTATACCCCACATTTACAGGCTTTAAAGCTGAATGTGGTCTTGGCAAAGCTCAAAACTGTCAATGAAAGCTATGAAAATTTACTCGACACGCGCACGAATGAAGGGCAAGCGACATTGATGATCGTGACCAAACCCCTCCGCAAGGAACTTGACGAACACTATGAAACACTCAGCACGAAACTTTTTGCTGTGAATGTAGCGAGTCCTACACCTGAGAGCGAGAAAGCGATAGCGGATTTGAATCGTTTGATCAAAGCGACGAAATTGGCTTGGAGGCAGAGGAACGGGTTGGTGCATTTGCATCCTGAGGATTTGGAAGATGAAAAAGAGAACGAAAGTGAAGGATAA", "aa": "MIDANGQLKKDPNSEEINKLDQERDQLLTYFFTKIAAEMRSPDQALEAAATALGDFLVVYRGIQGKANRQETQLIKSLLLDAEQAKYTPHLQALKLNVVLAKLKTVNESYENLLDTRTNEGQATLMIVTKPLRKELDEHYETLSTKLFAVNVASPTPESEKAIADLNRLIKATKLAWRQRNGLVHLHPEDLEDEKENESEG", "aa_hexdigest": "083fdd756531f6e92a11b74d9d663d57", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000DBF8436", "uniref100_id": "UniRef100_A0A328ECY6", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBF8436", "UniRef:UniRef100_A0A328ECY6" ] }, "ips": { "uniref100_id": "UniRef100_A0A328ECY6", "uniref90_id": "UniRef90_A0A328ECY6" }, "psc": { "uniref90_id": "UniRef90_A0A328ECY6", "product": "Family of unknown function (DUF6261)", "uniref50_id": "UniRef50_A0A328ECY6" }, "pscc": { "uniref50_id": "UniRef50_A0A328ECY6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328ECY6" ] }, "genes": [], "id": "NKOCPLAKPK_1795", "locus": "GCA003260355_01784" }, { "type": "cds", "sequence": "contig_2", "start": 91034, "stop": 91267, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4KG63", "UniRef:UniRef90_A0A328EAY0" ], "nt": "ATGCTCTGGAACGATATCATGACCGTGTTGAGAAAAATCTTTCATTTTCTTAAGTATGGAGGATGGAAAAAAGTGATAGGTTCCATCGTCCTTTTGCTACTCTTTGCTAATCTTTCTTTGATTAGCTTTTATACACGGTTTGCTTTTGAGGAACGGAGTCCGCAAGAAAGGAGTCAGTATTTTGGGTGTGATTACAACAAGAAGAAACGGCAACAAACGCTTTGTATTGCTTGA", "aa": "MLWNDIMTVLRKIFHFLKYGGWKKVIGSIVLLLLFANLSLISFYTRFAFEERSPQERSQYFGCDYNKKKRQQTLCIA", "aa_hexdigest": "e8cc09c235a3e302b5f980ccdfd58a80", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 91034, "stop": 91333, "observations": { "mutation": [ 91265 ], "stop": [ 91265 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EAY0", "query_cov": 1.0, "subject_cov": 0.7777777777777778, "identity": 1.0, "score": 157.0, "evalue": 1.25e-48, "gene_start": 1, "gene_end": 77, "reference_start": 1, "reference_end": 77, "reference_sequence": "MLWNDIMTVLRKIFHFLKYGGWKKVIGSIVLLLLFANLSLISFYTRFAFEERSPQERSQYFGCDYNKKKRQQTLCIAGDLQLEVFRRLHTLITSPFETK" }, "paralog": false, "identity": 0.98989898989899, "score": 200.0, "evalue": 4.66e-68, "description": "Internal stop codon at 91265. Nonsense mutation around 91265.", "psc": { "uniref90_id": "UniRef90_A0A328EAY0", "uniref50_id": "UniRef50_A0A2W4KG63", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EAY0", "UniRef:UniRef50_A0A2W4KG63" ] }, "pscc": { "uniref50_id": "UniRef50_A0A2W4KG63", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4KG63" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 9214.804499999991, "isoelectric_point": 9.79824352264404 }, "id": "NKOCPLAKPK_1796", "locus": "GCA003260355_01785" }, { "type": "cds", "sequence": "contig_2", "start": 91892, "stop": 92227, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAGAGATATAGGATTCAGCACAGGATTTTCTCTTTATGATTTTTCTTCATCTACAGGATTACGAATTAATTATCGTTTCTATAAGGAGAAAGATGAAAGATTTTATCCTGATAATCAGCTTTTTTGGCCACTAGTTTTGGAATTAGAAAGGAGCTCTTGTCTCAGAGTTGACTTTTCTTATTTAGTAGCTCCGAGTAAGTGCTTAAATTTAGTTATCAATCCGGTGAGAATTTCAGAACCGCATGCTCTTGTGATCGTGAGAAATACTTCAGAACAGTCTATTTCTTTTTCTTACCTAAAAGGTTATCTTCAGGAAGAGTATCAGAAAGAGTAA", "aa": "MRDIGFSTGFSLYDFSSSTGLRINYRFYKEKDERFYPDNQLFWPLVLELERSSCLRVDFSYLVAPSKCLNLVINPVRISEPHALVIVRNTSEQSISFSYLKGYLQEEYQKE", "aa_hexdigest": "98f082b0e8d77b0392eb166aa8b49bbc", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13094.704499999989, "isoelectric_point": 5.8510854721069325 }, "id": "NKOCPLAKPK_1797", "locus": "GCA003260355_01786" }, { "type": "cds", "sequence": "contig_2", "start": 92612, "stop": 92860, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCAAGCACAGACAGGAGATGATACTCCGATTGTTGTGATTACGGAAGCAAATAGCGGGGCAATTGTAACACAGACGGGAGATTCTCTCATCTATAGAAATGAAGTCTATGGATTTCAAGTAAAACTTGGGGAGGAGACTAAGGGATGTAAGCTCTTTGTGGATATAAATAGAGATAATATGCCTGATAATACTACTTTTCTTCTCTATGGAAAAATATGGACAGGAGAAGAGAGTCCTCTTGCCTGA", "aa": "MQAQTGDDTPIVVITEANSGAIVTQTGDSLIYRNEVYGFQVKLGEETKGCKLFVDINRDNMPDNTTFLLYGKIWTGEESPLA", "aa_hexdigest": "5f5d0e9b40be4edcbb6bfeb3493652f7", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9030.011699999997, "isoelectric_point": 4.273974037170411 }, "id": "NKOCPLAKPK_1798", "locus": "GCA003260355_01787" }, { "type": "cds", "sequence": "contig_2", "start": 93319, "stop": 93786, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EFJ8", "UniRef:UniRef90_A0A328EFJ8" ], "nt": "ATGGCGTATCGTATGAAACCTTTTCTGATAAGTATTGGAAGTCTGTGTGGGATCTTTCTTTTTTTGGTGGTAAGCAATGGGCTAGGGTTTGGGATGGAAGCGTTGTTTGGAGAGCATCTGGTGGTATGGATTTTGTTTGGGATTTTTTGGTTGTTCGGGGGAGCTCTTTGGGAAACGACGGACCAGAATACGAAGTTCTTTAAAAAACTTCTTATTATCGCAGAAGTCTATCTTTTTTTATTGATTTTCGTCCAGCCGGGGATCAATCTTTCATGGTTAGAGTTTTTGGTGCTGTATGGGGCAGGGTATGGGATTGTATATGAAGTAATGACCTTTAGAGCACAAAAGTGGCAGACGGTTTTCTTTTCTAGCTGTTATGGGGTGATCGTCGCATTGATGGTCAGCATTGCAGCGTTGATGTGGTGGAGGCAGCCCTTTGATGAGGAACAATTTATCACGCAGCAATGA", "aa": "MAYRMKPFLISIGSLCGIFLFLVVSNGLGFGMEALFGEHLVVWILFGIFWLFGGALWETTDQNTKFFKKLLIIAEVYLFLLIFVQPGINLSWLEFLVLYGAGYGIVYEVMTFRAQKWQTVFFSSCYGVIVALMVSIAALMWWRQPFDEEQFITQQ", "aa_hexdigest": "d06a2a65725f1f744c023650d252e151", "start_type": "ATG", "rbs_motif": "TTTA", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 93331, "stop": 94089, "observations": { "mutation": [ 93784 ], "stop": [ 93784 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EFJ8", "query_cov": 0.9741935483870968, "subject_cov": 0.412568306010929, "identity": 0.9540000000000001, "score": 290.0, "evalue": 5.38e-96, "gene_start": 5, "gene_end": 155, "reference_start": 1, "reference_end": 151, "reference_sequence": "MKPFLISIASLCGIFLFLVVSNGLGFGMEALFGEHLVVWILFGIFWLLGGALWETTDQNTKFFKKLLIIAEVYLFLLIFVQPGINLSWLEFVVLYAAGYGIVHEVMTFRAQKWQTVFFSSCYGVIVALMVSIAALMWWRQPFDEQQFIMQQGSFLFTVFDGRLVPSYTTILLKSPMSEKVISASTKSQTFLLDKNTDYLLQFASQQEEEHNFIVLEDQEGNLIKVGPQSQLRFSTRASNFSLQEQKGRVAFFGTGEFLPPELMEFRDRYQARQKAAALALLPAGLKSNPTFQRWSIRFTSSLAKAFPFWYGENAKYIQSYLPYLGLAATQKQHGKVDYSILKSTGKLGTEKANHKEKYREYFKKLF" }, "paralog": false, "identity": 0.9523809523809523, "score": 481.0, "evalue": 1.07e-173, "description": "Internal stop codon at 93784. Nonsense mutation around 93784.", "psc": { "uniref90_id": "UniRef90_A0A328EFJ8", "uniref50_id": "UniRef50_A0A328EFJ8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EFJ8", "UniRef:UniRef50_A0A328EFJ8" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EFJ8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EFJ8" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 17902.09089999999, "isoelectric_point": 5.270702552795409 }, "id": "NKOCPLAKPK_1799", "locus": "GCA003260355_01788" }, { "type": "cds", "sequence": "contig_2", "start": 93814, "stop": 94365, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EFJ8", "UniRef:UniRef90_A0A328EFJ8" ], "nt": "ATGCTTGTGCCCTCCTATACGACGATTCTTTTGAAGTCTCCTATGTCTGAAAAGGTGATTTCCGCAAGCACAAAAAGTCAGACCTTCTTATTAGAAAAAAATACTGATTATCTTCTTCAATTTGCCTCTCAGCAGGAGGAGGAGCATAATTTTATCGTGCTTCAAGATCAAGAAGGAAACCTCATCAAAGTAGGACCACAATCTCAGCTCAGGTTTTCTACTCATGCTTCGAATTTTTCCCTCCAAGAGCAAAAAGGAAGGGTAGCATTTTTTGGGACAGGAGAGCTTTTGCCTCCCGAGCTAATGGAATTTAGGGATCGTTATCAAGCACGTCAAAAGGCGGCAGCCCTTGCCTTGCTCCCGGCTGGTCTCAGAAGCAATCCTACATTTCAAAGATGGAGTATCAGGTTTACCTCCTCTTTGGCGAAAGTGTTTCCGTTTTGGTATGGAGAAAATGCGAAGTATATTCAGAGCTATCTGCCGTATCTGGGATTAGCAGCGACTCAGAAGCAGCATGGAAAGGTAGACTATTCAATTCTTAAATCCACGTGA", "aa": "MLVPSYTTILLKSPMSEKVISASTKSQTFLLEKNTDYLLQFASQQEEEHNFIVLQDQEGNLIKVGPQSQLRFSTHASNFSLQEQKGRVAFFGTGELLPPELMEFRDRYQARQKAAALALLPAGLRSNPTFQRWSIRFTSSLAKVFPFWYGENAKYIQSYLPYLGLAATQKQHGKVDYSILKST", "aa_hexdigest": "fb0c768bca3a65a4c6d60c876cd12221", "start_type": "ATG", "rbs_motif": "TTTA", "genes": [], "truncated": "both", "pseudogene": { "start": 93514, "stop": 94431, "observations": { "mutation": [ 93784, 94363, 94372 ], "stop": [ 93784, 94363, 94372 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EFJ8", "query_cov": 0.994535519125683, "subject_cov": 0.4972677595628415, "identity": 0.9670000000000001, "score": 344.0, "evalue": 9.55e-117, "gene_start": 2, "gene_end": 183, "reference_start": 163, "reference_end": 344, "reference_sequence": "MKPFLISIASLCGIFLFLVVSNGLGFGMEALFGEHLVVWILFGIFWLLGGALWETTDQNTKFFKKLLIIAEVYLFLLIFVQPGINLSWLEFVVLYAAGYGIVHEVMTFRAQKWQTVFFSSCYGVIVALMVSIAALMWWRQPFDEQQFIMQQGSFLFTVFDGRLVPSYTTILLKSPMSEKVISASTKSQTFLLDKNTDYLLQFASQQEEEHNFIVLEDQEGNLIKVGPQSQLRFSTRASNFSLQEQKGRVAFFGTGEFLPPELMEFRDRYQARQKAAALALLPAGLKSNPTFQRWSIRFTSSLAKAFPFWYGENAKYIQSYLPYLGLAATQKQHGKVDYSILKSTGKLGTEKANHKEKYREYFKKLF" }, "paralog": false, "identity": 0.9475409836065574, "score": 577.0, "evalue": 5.91e-211, "description": "Internal stop codon at 93784, 94363, 94372. Nonsense mutation around 93784, 94363, 94372.", "psc": { "uniref90_id": "UniRef90_A0A328EFJ8", "uniref50_id": "UniRef50_A0A328EFJ8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EFJ8", "UniRef:UniRef50_A0A328EFJ8" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EFJ8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EFJ8" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 20820.520199999995, "isoelectric_point": 9.395960807800293 }, "id": "NKOCPLAKPK_1800", "locus": "GCA003260355_01789" }, { "type": "cds", "sequence": "contig_2", "start": 94806, "stop": 94976, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4IGT3", "UniRef:UniRef90_A0A328EC63" ], "nt": "ATGCAGAAACCCATCGTCCTCCCAGAATATCAACATATGAATTTTGTTTTTTGTGTTGTGCTGATTGTTATTTCTCTTTATATCTCGATTTTTTACGGGATTTATCCGATTCATGTGAGGTTTAGTAGAGCTACGCTTTTTGTCTTGGGGATCTCTGCTATTGTGATGTGA", "aa": "MQKPIVLPEYQHMNFVFCVVLIVISLYISIFYGIYPIHVRFSRATLFVLGISAIVM", "aa_hexdigest": "e7998fe876a7de6ddbe045ddc3996e48", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 94728, "stop": 95138, "observations": { "mutation": [ 94782, 94974, 95061 ], "stop": [ 94782, 94974, 95061 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EC63", "query_cov": 1.0, "subject_cov": 0.4117647058823529, "identity": 1.0, "score": 106.0, "evalue": 2.83e-28, "gene_start": 1, "gene_end": 56, "reference_start": 27, "reference_end": 82, "reference_sequence": "MRDALKRIGVITAFGTFSGYLIYSLIMQKPIVLPEYQHMNFVFCVVLIVISLYISIFYGIYPIHVRFSRATLFVLGISAIVMGKTVFLNDPMNDAYFGDLSCVVGVLLLIIGPTNFIMTSGVKKKREEKNLEIIEV" }, "paralog": false, "identity": 0.9779411764705882, "score": 257.0, "evalue": 2.55e-90, "description": "Internal stop codon at 94782, 94974, 95061. Nonsense mutation around 94782, 94974, 95061.", "psc": { "uniref90_id": "UniRef90_A0A328EC63", "uniref50_id": "UniRef50_A0A2W4IGT3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EC63", "UniRef:UniRef50_A0A2W4IGT3" ] }, "pscc": { "uniref50_id": "UniRef50_A0A2W4IGT3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4IGT3" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 6491.876799999998, "isoelectric_point": 9.034808921813966 }, "id": "NKOCPLAKPK_1801", "locus": "GCA003260355_01790" }, { "type": "tRNA", "sequence": "contig_2", "start": 95176, "stop": 95252, "strand": "+", "gene": "trnG", "product": "tRNA-Gly(gcc)", "amino_acid": "Gly", "anti_codon": "gcc", "score": 77.0, "nt": "GCGGGTGTAGCTCATTTGGCTAGAGCGCTTCCTTGCCAAGGAAGAGGCAGCGGGTTCGAGTCCCGTCACTCGCTCCA", "db_xrefs": [ "SO:0000260" ], "anti_codon_pos": [ 95210, 95212 ], "id": "NKOCPLAKPK_30", "locus": "GCA003260355_01791" }, { "type": "tRNA", "sequence": "contig_2", "start": 95306, "stop": 95389, "strand": "+", "gene": "trnL", "product": "tRNA-Leu(caa)", "amino_acid": "Leu", "anti_codon": "caa", "score": 58.2, "nt": "GCCTAGGTGATGGAACTGGTAGACATGCTAGACTCAAAATCTGGTGGCTTCACGGCCGTGCGGGTTCAAGTCCCGCTCTAGGTA", "db_xrefs": [ "SO:0000264" ], "anti_codon_pos": [ 95340, 95342 ], "id": "NKOCPLAKPK_31", "locus": "GCA003260355_01792" }, { "type": "cds", "sequence": "contig_2", "start": 95593, "stop": 95808, "strand": "+", "frame": 1, "gene": null, "product": "Single-stranded DNA-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1V5ZP08" ], "nt": "ATGGATTTGAATAAGGTAATGTTGATTGGGAGGTCTACGAGTACTTTACAGGTCAAGACTATAGAGAGCACTCAAAGTGCTGTCGTTAATTTCACTCTTGCAACCAATAGAAAGTATAAAAATAAAGAAGGAAATGTTTTGGAAGATGCTGAATATCATAGATGTGTTGCCTATGGGAATACTGCAGAAGTGCTTGGAAAGTATCTTACTAAGTGA", "aa": "MDLNKVMLIGRSTSTLQVKTIESTQSAVVNFTLATNRKYKNKEGNVLEDAEYHRCVAYGNTAEVLGKYLTK", "aa_hexdigest": "887b42169ba29fcf944e33b2a5b9d39a", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A1V5ZP08", "query_cov": 0.8309859154929577, "subject_cov": 0.9833333333333333, "identity": 0.746, "score": 93.6, "evalue": 6.8e-24, "valid": false, "product": "Single-stranded DNA-binding protein", "uniref50_id": "UniRef50_A0A1V5ZP08" }, "pscc": { "uniref50_id": "UniRef50_A0A1V5ZP08", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1V5ZP08" ], "product": "Single-stranded DNA-binding protein" }, "genes": [], "id": "NKOCPLAKPK_1802", "locus": "GCA003260355_01793" }, { "type": "cds", "sequence": "contig_2", "start": 95887, "stop": 95988, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATTATTGATAATTTTATTTTCCTTGATACGAAAGTTTCGAACTCAGATGAAGTAGAGATTGATCCGCTCTCTGATTTTGATAGTGAGGTTCCTTTTTAA", "aa": "MIIDNFIFLDTKVSNSDEVEIDPLSDFDSEVPF", "aa_hexdigest": "ef8ecd9f0775d0e77d07f68afb596234", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3792.1324999999997, "isoelectric_point": 4.0500284194946286 }, "id": "NKOCPLAKPK_1803", "locus": "GCA003260355_01794" }, { "type": "cds", "sequence": "contig_2", "start": 96897, "stop": 97013, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCTGTTTATCATTTATCTTTTTGTTATTGCTCCGCTGTTTAAGTATTATTTGGACTATGTTATGGATTTTTCTCTCGTTACTCCCGAGTATCTTACGAGATACAATCAGACTTGA", "aa": "MLFIIYLFVIAPLFKYYLDYVMDFSLVTPEYLTRYNQT", "aa_hexdigest": "97e535eb474ced2a94f4bdb6e57762fd", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4705.529000000001, "isoelectric_point": 4.556577491760254 }, "id": "NKOCPLAKPK_1804", "locus": "GCA003260355_01795" }, { "type": "cds", "sequence": "contig_2", "start": 97329, "stop": 97589, "strand": "+", "frame": 3, "gene": null, "product": "SHSP domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A288D9U8", "UniRef:UniRef90_A0A328ECX6" ], "nt": "GTGAAAAAGGAAACTATACGTCTTACTATTGAAGATTATAAGCTTATTCTTGAGGGAGAAAGGATTTTAACACCTTTAAAAGAGAATCTTTTGCCTGTGAAAGAAGAATGTTATCGAGGAAAGATTAGACAAATCATCGATCTTCCTGCTCAGATTTATTTTGATAGAATTCATAGTAAACTTTCTCCGGAAAATATTTTGTGGATTATTATTCCCAAAGCTCTTGTTCCTGAAAAGATAACGCTCCAGATTGAAGAATAG", "aa": "MKKETIRLTIEDYKLILEGERILTPLKENLLPVKEECYRGKIRQIIDLPAQIYFDRIHSKLSPENILWIIIPKALVPEKITLQIEE", "aa_hexdigest": "6d3da6f1baa1a27056aa0dc013cfae89", "start_type": "GTG", "rbs_motif": "TTATT", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 97245, "stop": 97589, "observations": { "mutation": [ 97323 ], "stop": [ 97323 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328ECX6", "query_cov": 1.0, "subject_cov": 0.7543859649122807, "identity": 0.988, "score": 172.0, "evalue": 3.27e-54, "gene_start": 1, "gene_end": 86, "reference_start": 29, "reference_end": 114, "reference_sequence": "MQLDNSPLIPYDIYESSQEIVIIMPLGGVKKETIRLTIEDYKLILEGERILTPLKENLLPVKEECYRGKIRQIIDLPAQIYFDRIHSKLSPENILWIIIPKALVPEKITLQIEE" }, "paralog": false, "identity": 0.9912280701754386, "score": 223.0, "evalue": 1.17e-76, "description": "Internal stop codon at 97323. Nonsense mutation around 97323.", "psc": { "uniref90_id": "UniRef90_A0A328ECX6", "product": "SHSP domain-containing protein", "uniref50_id": "UniRef50_A0A288D9U8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328ECX6", "UniRef:UniRef50_A0A288D9U8" ] }, "pscc": { "uniref50_id": "UniRef50_A0A288D9U8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A288D9U8" ], "product": "SHSP domain-containing protein" } }, "id": "NKOCPLAKPK_1805", "locus": "GCA003260355_01796" }, { "type": "cds", "sequence": "contig_2", "start": 97596, "stop": 97883, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTCAAAACAGAATATTCTCCACGATACCAACAAACAAAAGTAAGTGAATACTTTTCCCTTGCTAAAAAGATGAAGTCAGATCTAAAAAATAATATTTTCTCAACCAACATAGACGAGGATAACTATGAATGGAAAGCTTTTCCAAAAATCTTTGAAAAAGAAATAGATCAAAGCCTGATCGATACCTCCGAAAAAATCTTAAAAAAAATCTTCTCCTCAGAAATAAGATCACACTATGAAGAGAAGAAACAACAACGAATAGCAGCACTTCCAGACACAATCTAA", "aa": "MLKTEYSPRYQQTKVSEYFSLAKKMKSDLKNNIFSTNIDEDNYEWKAFPKIFEKEIDQSLIDTSEKILKKIFSSEIRSHYEEKKQQRIAALPDTI", "aa_hexdigest": "36e9e8f894414e15bef8533da703258e", "start_type": "ATG", "rbs_motif": "TTTTT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11358.783899999988, "isoelectric_point": 7.863869667053223 }, "id": "NKOCPLAKPK_1806", "locus": "GCA003260355_01797" }, { "type": "cds", "sequence": "contig_2", "start": 99963, "stop": 100121, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAACAGGAACGATTTTAGCCAAAGAAATAATTTTAGAAAAAACAGATTTTCGAGAAAAAAACAATACCTTCATCATCAGTAAAGATGAAACACGATACGACAAAGGAATCGACAGTCAATCTAATCCTGACTTTAATCTACTATATCAGACTTGA", "aa": "MLTGTILAKEIILEKTDFREKNNTFIISKDETRYDKGIDSQSNPDFNLLYQT", "aa_hexdigest": "495c435e0d1271e1aa349b34fde32b92", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6069.758400000002, "isoelectric_point": 4.810363578796387 }, "id": "NKOCPLAKPK_1807", "locus": "GCA003260355_01798" }, { "type": "cds", "sequence": "contig_2", "start": 101041, "stop": 101196, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTTCTATGTTTTCTCCAGTTATTGAATTGTTTGTTTCTGTGATATTTCAAGAAAAAGATTCTTCTGATTTCTTTTGTGATGACTGGATTTCTGGCTTATTTTCTTCTGCTCAGGTTTGTCAAACTTCCTTTGAAATCGTTGGTTCTGGATGA", "aa": "MFSMFSPVIELFVSVIFQEKDSSDFFCDDWISGLFSSAQVCQTSFEIVGSG", "aa_hexdigest": "b6870961f758aa86806ff073bfc7ff2e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5696.369399999999, "isoelectric_point": 4.0500284194946286 }, "id": "NKOCPLAKPK_1808", "locus": "GCA003260355_01799" }, { "type": "cds", "sequence": "contig_2", "start": 101163, "stop": 101276, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAATCCTTGCATTTCTGCTGATCACTTCGCTTTTTTTTCAGCCACTTGCAATTTTTGCAGAAGAAATGGAATCTCATCCAGAACCAACGATTTCAAAGGAAGTTTGA", "aa": "MKKILAFLLITSLFFQPLAIFAEEMESHPEPTISKEV", "aa_hexdigest": "4d800ea9defc75cba64d0c8c58e8f2b9", "start_type": "ATG", "rbs_motif": "TTATT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4250.0263, "isoelectric_point": 5.012824058532714 }, "id": "NKOCPLAKPK_1809", "locus": "GCA003260355_01800" }, { "type": "cds", "sequence": "contig_2", "start": 101429, "stop": 101617, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATTATAGATTTATTGATTTCCCTACTGATTGGTCTCAACCTAAAAACTCTAAAGATGATGTGAAGGTGCCCGAGAAGATTCTATCTTCACAACAAAAAACTAAAGAGAAGATTCAGACTTTCCCTGATAAAAAACTAGAACCAGTGGAGAAAAAGGATTCTTCATTAGATTTTGTTAAAGAATGA", "aa": "MNYRFIDFPTDWSQPKNSKDDVKVPEKILSSQQKTKEKIQTFPDKKLEPVEKKDSSLDFVKE", "aa_hexdigest": "f7fe31dbbaeaa40f1154aad93d26bb24", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7360.267800000002, "isoelectric_point": 7.926984214782716 }, "id": "NKOCPLAKPK_1810", "locus": "GCA003260355_01801" }, { "type": "cds", "sequence": "contig_2", "start": 101660, "stop": 102340, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGAGCTTGTGACTTTGGATACTCTTGATGGAACTCCGCTCAAAAATACCATGCTTTCTATATTGAAGAATCCTCAATTTGATGATTATCCTGATTTGAAAGGAAAAACTCCAGAACAAAGGTTAGAGTTTGTTTTTAGGAAGATAAATATTGCTTTAACAAAGTTTTATGCTAGAAAACTAAATATTGGGCTGACTGAACCAGTTCCAGATTATCTCAAGAAGGTAGTTATTCCTGCTACGGAATTGTTTTTGATGGAGACTTTGGCTCAAATGGGAAATGAGACGAATATCAATTTTTTGGGACAGCTATCTAGTATGGATTTTAGTAGTTTAGGGAATCTTGTAACCCAGGTAAAAGATTTTTCTGAGAAGTTTTCGTTTTCTTTTGGTCAAGGAAAGACGCTTTTGAATCTTACAGACTTTCTTGCTTTACCAAAAAATCAAGCTCAATTAAGTAAGTTGGATGATCCTTATGAATTCTATCAAAAAGTGCTCAAAAATCCTCTCTGGAAGCAAGATATTACGACAAAGGATAGTCCAAAACCGAATCAAATTTCACTTGATTCAATTACTTGGGAACAGTTTGATTTGCGTGCTCTTAAAAATAACCTTTCTAATGAAGAGAAACAGCAGGCTTTCCAAATGGGAAAGGAAAAAATGAAACAACAGCTTTGA", "aa": "MNELVTLDTLDGTPLKNTMLSILKNPQFDDYPDLKGKTPEQRLEFVFRKINIALTKFYARKLNIGLTEPVPDYLKKVVIPATELFLMETLAQMGNETNINFLGQLSSMDFSSLGNLVTQVKDFSEKFSFSFGQGKTLLNLTDFLALPKNQAQLSKLDDPYEFYQKVLKNPLWKQDITTKDSPKPNQISLDSITWEQFDLRALKNNLSNEEKQQAFQMGKEKMKQQL", "aa_hexdigest": "41ae3e39e17ab48dacaedddbb78427db", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 25984.590900000003, "isoelectric_point": 6.404924583435059 }, "id": "NKOCPLAKPK_1811", "locus": "GCA003260355_01802" }, { "type": "cds", "sequence": "contig_2", "start": 102392, "stop": 103351, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAAAAAGCGGACGGTGTTTTTCAGAATATTGATGCTCTAGGAAATAAACTACTTGATAGTATGGATATTTTGGGAAATACTTCAAAAGTTTTGAAATCAACGCTAGGATTTGATCTTTGGAAACAAATCAGGAAAACTCCAGCACTTGGAGGTGCTCTTAATGTTGTTTTAGGTTTGCTTGGTTTCTCTGGAGGAGTTGAGGGATTGGAAAGAGCTTGGAAAAAAAGGATTCTTGAGAGGGAGCTTAAACAGCCTCAGAAAGCATATATTGCTGAGTCTTATAAAAATTATATGCAGAAGAAAGCTCTTCAAGCTACTACGGCTAAAACACTTCTTGATCAACATAATCTCAAAGTGGAGGATGCTGCTCAGCAGAAGTTTGCTCTGGATATGCCTCTGATAAAAAGTCAGATTCTTCAAAAAATAGATGAAAAACCTGAACTTTTAAATCTTTCTACTTTGAAAATGACTAAAAGTCCTGTTTTTAAGGGAGAAGATTTTGTGCAAGAAGCAAAGGTTGGAGAAAAAACGAAGCTTACTCTCAAAAGAAAACTCTCTCCACAGGAAAGAGAAGCTTTTGTGGATACTTATCTCCATGAGGGATTGGAGCGTTTTGCTTCGAATCCTAAAGCCTTATCTTCACTACCTAATGCTGATACTTTAGCGTTTATTCTCCTCTCAGGGGTTGCTCTTAATAAAGATGATGTGATAGATGGTGTGGAGGCTCAGGTTTTTCTACCTGATCAGTATACTGATGCAGCACTAGCAACTCAAAATGGGCAGAATAGATATATTTATCCTAATGGATCTACTCCTATTGGAAATAATAAGCCTGTAGTGAATCCTATTTCTCCAAATCCTTCTGCAGAAAATATCAATAATTTTGTGGAGAATATTGGTCAAAAACCAGAATCTGGCAATGATATAAACAATGATCAGTTAGACGAAGCTTTGTGA", "aa": "MEKADGVFQNIDALGNKLLDSMDILGNTSKVLKSTLGFDLWKQIRKTPALGGALNVVLGLLGFSGGVEGLERAWKKRILERELKQPQKAYIAESYKNYMQKKALQATTAKTLLDQHNLKVEDAAQQKFALDMPLIKSQILQKIDEKPELLNLSTLKMTKSPVFKGEDFVQEAKVGEKTKLTLKRKLSPQEREAFVDTYLHEGLERFASNPKALSSLPNADTLAFILLSGVALNKDDVIDGVEAQVFLPDQYTDAALATQNGQNRYIYPNGSTPIGNNKPVVNPISPNPSAENINNFVENIGQKPESGNDINNDQLDEAL", "aa_hexdigest": "4cb35c3691ba457e51033e3d43911d56", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 35222.732699999986, "isoelectric_point": 5.905025672912598 }, "id": "NKOCPLAKPK_1812", "locus": "GCA003260355_01803" }, { "type": "cds", "sequence": "contig_2", "start": 103565, "stop": 103840, "strand": "+", "frame": 2, "gene": null, "product": "Transglycosylase SLT domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EEZ0", "UniRef:UniRef90_A0A328EGL5" ], "nt": "ATGCCAAGTACTGCTACAAAGTTGGGAACAACAGTTTCTGCTCTTAGATCTATGTCTGCTGTTCAACAGCTTGATTATGTGAAAAAGTATTATCAACTTTCTCCAGGGAAAAAGTTTAGGTCTCTCAAAGATCTTTATCTCTATACTTTTTTCCCGATAGCTATGAATCATTCAAGTAATCCTAATTATGTTTTTCAGTCTAGTACTATTTCTGCTGCAAAACTTGCAGGGTTGCATAGAAAGTTAGCTAGAGGGAAAAGCTATATCACTATGTGA", "aa": "MPSTATKLGTTVSALRSMSAVQQLDYVKKYYQLSPGKKFRSLKDLYLYTFFPIAMNHSSNPNYVFQSSTISAAKLAGLHRKLARGKSYITM", "aa_hexdigest": "95c8f9a2911b8c8099e9770dcd750821", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 103433, "stop": 103912, "observations": { "mutation": [ 103502, 103541, 103838 ], "stop": [ 103502, 103541, 103838 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EGL5", "query_cov": 1.0, "subject_cov": 0.5723270440251572, "identity": 1.0, "score": 177.0, "evalue": 2.5e-55, "gene_start": 1, "gene_end": 91, "reference_start": 45, "reference_end": 135, "reference_sequence": "MEKIAAELDINPNRLMALMASETGGTFNPAIANKSTGATGLIQIMPSTATKLGTTVSALRSMSAVQQLDYVKKYYQLSPGKKFRSLKDLYLYTFFPIAMNHSSNPNYVFQSSTISAAKLAGLHRKLARGKSYITMGDFNYYISGMVNQNVPVEFRNQFA" }, "paralog": false, "identity": 0.9811320754716981, "score": 303.0, "evalue": 2.3e-107, "description": "Internal stop codon at 103502, 103541, 103838. Nonsense mutation around 103502, 103541, 103838.", "psc": { "uniref90_id": "UniRef90_A0A328EGL5", "product": "Transglycosylase SLT domain-containing protein", "uniref50_id": "UniRef50_A0A328EEZ0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EGL5", "UniRef:UniRef50_A0A328EEZ0" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EEZ0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EEZ0" ], "product": "Transglycosylase SLT domain-containing protein" } }, "id": "NKOCPLAKPK_1813", "locus": "GCA003260355_01804" }, { "type": "cds", "sequence": "contig_2", "start": 104499, "stop": 104651, "strand": "-", "frame": 3, "gene": null, "product": "DUF4134 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328ECV9", "UniRef:UniRef90_A0A328ECV9" ], "nt": "ATGGTCAAAAAAGTATTCTCCACCGTCATCCTCGCATTGTTTGCCACGCTCCCTGTCTTCGGGATCAAAGTTACTGTTCCTCAAGGACACGCAGGAGACGATCTTCCAAAAAAACACGTCGTCGATACCCAAGAGGAAGGAATATTTCAGTGA", "aa": "MVKKVFSTVILALFATLPVFGIKVTVPQGHAGDDLPKKHVVDTQEEGIFQ", "aa_hexdigest": "906753fc2cb02f07ebe43b68676f44e4", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 104295, "stop": 104651, "observations": { "mutation": [ 104357, 104501 ], "stop": [ 104357, 104501 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328ECV9", "query_cov": 1.0, "subject_cov": 0.423728813559322, "identity": 0.96, "score": 98.6, "evalue": 1.45e-25, "gene_start": 1, "gene_end": 50, "reference_start": 1, "reference_end": 50, "reference_sequence": "MAKKIFSTVILALFATLPVFGIKVTVPQGHAGDDLPKKHVVDTQEEGIFQGINIINKYLRWSFAVVCTAVVVYGGYQLITANGDKKAMKKAVWALMGAGIGITIAMLSYTIVSLLTNL" }, "paralog": false, "identity": 0.9576271186440678, "score": 224.0, "evalue": 9.35e-78, "description": "Internal stop codon at 104357, 104501. Nonsense mutation around 104357, 104501.", "psc": { "uniref90_id": "UniRef90_A0A328ECV9", "product": "DUF4134 domain-containing protein", "uniref50_id": "UniRef50_A0A328ECV9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328ECV9", "UniRef:UniRef50_A0A328ECV9" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328ECV9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328ECV9" ], "product": "DUF4134 domain-containing protein" } }, "id": "NKOCPLAKPK_1814", "locus": "GCA003260355_01805" }, { "type": "cds", "sequence": "contig_2", "start": 104862, "stop": 105320, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATCATGGTTACTGCTAGGACCATTGTCATTCTGAGCAAGAACTTTGAAATACTAATAATAAGTGATGGTCTACGATTGATTCCTTTATTTTTTCATCAGATTTTTATTTCTGTTCCTTCTTTTAGGATGTATTTTCCTACACTCATTTTGTCAGTTCCAATACTATCTCATCCTACGATAGCATCAGATTGGATGGCAGGTGTAAATGCTTGCTCAAGAAAATCTCAGTTAGTATTTCCTGCTTGTCCTTTTGCGTATCCGATTCATAGAGAAAAGACTGCTATACTTCCTAAGCTACTAAGAATAAAACTAAAAATTCTGATTTTCTTGGCTATTGATATTTTCATATTTACATTTGAGTCAGTAAATTGTCTGCTTCAATCATTTCTTGTTCTCTTGCTTCGTTTTCTTGTGCATGGATTTTTGCCTGTGCTTGGCTATAGAGTGCAGGATTGA", "aa": "MIMVTARTIVILSKNFEILIISDGLRLIPLFFHQIFISVPSFRMYFPTLILSVPILSHPTIASDWMAGVNACSRKSQLVFPACPFAYPIHREKTAILPKLLRIKLKILIFLAIDIFIFTFESVNCLLQSFLVLLLRFLVHGFLPVLGYRVQD", "aa_hexdigest": "69bebe635a2a516795dcbefb1ad40091", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17406.986799999984, "isoelectric_point": 9.779547691345211 }, "id": "NKOCPLAKPK_1815", "locus": "GCA003260355_01806" }, { "type": "cds", "sequence": "contig_2", "start": 105214, "stop": 105618, "strand": "-", "frame": 2, "gene": null, "product": "Rho termination factor N-terminal domain-containing protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DC050F9", "UniRef:UniRef100_A0A328EBI0", "UniRef:UniRef50_A0A3D0USM5", "UniRef:UniRef90_A0A328EBI0" ], "nt": "ATGAATCCAGTATTACAGCAGTATCTACCACAAGAGCTACGGGAAATCGCAGCAGATTTCAAAATTCCTGAAGCCTTCTTGGTAAATAACTCAAACCTTATTCAGCTTATCCTAAAATCAAAATCTCTCGCCGAATACGAAGAGAAACAGAATTGGTTTAATCTTTTACCAATTATGTCTCCTGAACAAATAGAAAAACTCAGAGATATTCTCACAAGAGAACAACAAAAACTTGAGGAAATCAACCAAAAATACAGCCAAAAACAGGCAGAAATTTCCGAAAAATACCAGCAAAGTTTCAATCCTGCACTCTATAGCCAAGCACAGGCAAAAATCCATGCACAAGAAAACGAAGCAAGAGAACAAGAAATGATTGAAGCAGACAATTTACTGACTCAAATGTAA", "aa": "MNPVLQQYLPQELREIAADFKIPEAFLVNNSNLIQLILKSKSLAEYEEKQNWFNLLPIMSPEQIEKLRDILTREQQKLEEINQKYSQKQAEISEKYQQSFNPALYSQAQAKIHAQENEAREQEMIEADNLLTQM", "aa_hexdigest": "afab72393a091aba20dbd03a6ef93699", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000DC050F9", "uniref100_id": "UniRef100_A0A328EBI0", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DC050F9", "UniRef:UniRef100_A0A328EBI0" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EBI0", "uniref90_id": "UniRef90_A0A328EBI0" }, "psc": { "uniref90_id": "UniRef90_A0A328EBI0", "uniref50_id": "UniRef50_A0A3D0USM5" }, "pscc": { "uniref50_id": "UniRef50_A0A3D0USM5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3D0USM5" ], "product": "Rho termination factor N-terminal domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_1816", "locus": "GCA003260355_01807" }, { "type": "cds", "sequence": "contig_2", "start": 105703, "stop": 105858, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EC72", "UniRef:UniRef90_A0A328EC72" ], "nt": "ATGAAAAGACTTTCAAGGGATGAGATCAGAAAAATGAAAGAAAATATGCAAAAAACACCACTCATCCAAAAGCAAGCTGAACAATATCACAATAAAGAAAGTAAAGAAGCTGAAGCATTATTGAACAAAATAGAGATTCCTTCAGAAAAACAATGA", "aa": "MKRLSRDEIRKMKENMQKTPLIQKQAEQYHNKESKEAEALLNKIEIPSEKQ", "aa_hexdigest": "c17ea922c44117ce22b97c3df465439f", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 105634, "stop": 105888, "observations": { "mutation": [ 105669, 105702, 105705, 105870 ], "stop": [ 105669, 105702, 105705, 105870 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EC72", "query_cov": 1.0, "subject_cov": 0.6, "identity": 1.0, "score": 93.6, "evalue": 5.74e-24, "gene_start": 1, "gene_end": 51, "reference_start": 11, "reference_end": 61, "reference_sequence": "MQETYSGKSGMKRLSRDEIRKMKENMQKTPLIQKQAEQYHNKESKEAEALLNKIEIPSEKQGGKLDIQPPKKIGFFQKIKNYLFG" }, "paralog": false, "identity": 0.9523809523809523, "score": 154.0, "evalue": 1.32e-50, "description": "Internal stop codon at 105669, 105702, 105705, 105870. Nonsense mutation around 105669, 105702, 105705, 105870.", "psc": { "uniref90_id": "UniRef90_A0A328EC72", "uniref50_id": "UniRef50_A0A328EC72", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EC72", "UniRef:UniRef50_A0A328EC72" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EC72", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EC72" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 6140.0374999999985, "isoelectric_point": 9.397121238708493 }, "id": "NKOCPLAKPK_1817", "locus": "GCA003260355_01808" }, { "type": "cds", "sequence": "contig_2", "start": 105932, "stop": 106093, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATCTTGGACAAGTTGATTCGACGATCGAATCCTCAAATCCACTAGGAATGATTTCAATGAAACAATATGCAAAGAAACTCGTAGATAGACAACTCATCAACCCTAAAGACGTAAAACATCTCTTTAGAGTAGAAGAAAGAGCAACCGAAACAGACTAA", "aa": "MDLGQVDSTIESSNPLGMISMKQYAKKLVDRQLINPKDVKHLFRVEERATETD", "aa_hexdigest": "e6d1784cae78b0150b8e268eb0ca5e11", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6076.8885, "isoelectric_point": 5.697506523132325 }, "id": "NKOCPLAKPK_1818", "locus": "GCA003260355_01809" }, { "type": "cds", "sequence": "contig_2", "start": 106145, "stop": 106600, "strand": "-", "frame": 1, "gene": "pilT", "product": "Type IV pilus twitching motility protein PilT", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A948C4U4" ], "nt": "GTGACAGGATCTACAGGAAGCGGAAAATCAACATCACTAGTAAGTATGATTGAATATATCAACAAAACAAGAAACGAAAACATCATCACGATCGAAGACCCTATCGAATATATCTTTGAAGGAGATAAATGTAGTATTTCACAGAGACAGGTAGGACATGATACCTGGTCATTCAAAAACGCACTAAAAGCTGTAATGAGAGAAGATCCGGATATTATTTTTGTAGGAGAAATTCGTGATACTGAAACTGCAGAAACCGTCCTTAGCCTAGCGGAAAGTGGACACCTTGTTTTTAGCACCTTGCACACAAGCTCAGCAAGTCATAGTTTGAGTAGATTCATGTCCTTTTTCCCTACAAATATGGAACAAAGTATCGCCGACAGACTTTCAGATAGTCTCATCGGAATCCAATCTCAAATGCTCGTAAAAAGAACAGATACCAATACGAGAATCTGA", "aa": "MTGSTGSGKSTSLVSMIEYINKTRNENIITIEDPIEYIFEGDKCSISQRQVGHDTWSFKNALKAVMREDPDIIFVGEIRDTETAETVLSLAESGHLVFSTLHTSSASHSLSRFMSFFPTNMEQSIADRLSDSLIGIQSQMLVKRTDTNTRI", "aa_hexdigest": "7dde012e6996d6a77c0907d4d9022057", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A948C4U4", "query_cov": 0.9006622516556292, "subject_cov": 0.8, "identity": 0.662, "score": 191.0, "evalue": 6.56e-60, "valid": false, "product": "Type IV pilus twitching motility protein PilT", "uniref50_id": "UniRef50_A0A948C4U4" }, "pscc": { "uniref50_id": "UniRef50_A0A948C4U4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A948C4U4" ], "product": "Type IV pilus twitching motility protein PilT" }, "genes": [ "pilT" ], "id": "NKOCPLAKPK_1819", "locus": "GCA003260355_01810" }, { "type": "cds", "sequence": "contig_2", "start": 106548, "stop": 106709, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGGAAAATCAATTCTCGCATCAGAACGCTCGAAGAGATGATGTTCTCTAATCTCGCCGAAAGTATAAAAAGGAACGTCCTTGCAGCAAAAAAAAGGACTTTTCCTAGTGACAGGATCTACAGGAAGCGGAAAATCAACATCACTAGTAAGTATGATTGA", "aa": "MRKINSRIRTLEEMMFSNLAESIKRNVLAAKKRTFPSDRIYRKRKINITSKYD", "aa_hexdigest": "7a16c23509988bf4dfd988ad2d22b1bc", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6419.5127, "isoelectric_point": 11.028171348571778 }, "id": "NKOCPLAKPK_1820", "locus": "GCA003260355_01811" }, { "type": "cds", "sequence": "contig_2", "start": 106725, "stop": 107021, "strand": "-", "frame": 3, "gene": null, "product": "Bacterial type II secretion system protein E domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328ECY0", "UniRef:UniRef90_A0A328ECY0" ], "nt": "ATGAACACAGTAAGAGTCGATATTGATTCCATTTTAGCCGGGATTGCAGAAAACCCGAATATTTCCGATCTTCATCTAACCTGCTCCGGGAAAAGTTCATATAGACTGAACGGGGATATCATCAAAGATGAAAAAATTCCGATCATCAACAGCGAAAACATGGAAATCATCCTCAAACAACTCTTTCAAAATAATGCCCAAAATTTTGATAAGTTTATCTGCGACAAAGAAAGCGATTTTGCATATGAGGGAAAAGATGGAACCTCTTATAGAGTAAATGCCTTTTTTGAAAAATGA", "aa": "MNTVRVDIDSILAGIAENPNISDLHLTCSGKSSYRLNGDIIKDEKIPIINSENMEIILKQLFQNNAQNFDKFICDKESDFAYEGKDGTSYRVNAFFEK", "aa_hexdigest": "05a49cdd89afdbd1acc616718d46c328", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 106473, "stop": 107021, "observations": { "mutation": [ 106484, 106550, 106718, 106727 ], "stop": [ 106484, 106550, 106718, 106727 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328ECY0", "query_cov": 1.0, "subject_cov": 0.5384615384615384, "identity": 1.0, "score": 198.0, "evalue": 2.95e-63, "gene_start": 1, "gene_end": 98, "reference_start": 1, "reference_end": 98, "reference_sequence": "MNTVRVDIDSILAGIAENPNISDLHLTCSGKSSYRLNGDIIKDEKIPIINSENMEIILKQLFQNNAQNFDKFICDKESDFAYEGKDGTSYRVNAFFEKGKIGIVMRKINSRIRTLEEMMFSNLAESIKRNVLAAKKRTFPSDRIYRKRKINITSKYDGIYQQNKKRKHHHDRRPYRIYLGRR" }, "paralog": false, "identity": 0.978021978021978, "score": 351.0, "evalue": 6.44e-126, "description": "Internal stop codon at 106484, 106550, 106718, 106727. Nonsense mutation around 106484, 106550, 106718, 106727.", "psc": { "uniref90_id": "UniRef90_A0A328ECY0", "product": "Bacterial type II secretion system protein E domain-containing protein", "uniref50_id": "UniRef50_A0A328ECY0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328ECY0", "UniRef:UniRef50_A0A328ECY0" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328ECY0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328ECY0" ], "product": "Bacterial type II secretion system protein E domain-containing protein" } }, "id": "NKOCPLAKPK_1821", "locus": "GCA003260355_01812" }, { "type": "cds", "sequence": "contig_2", "start": 107292, "stop": 107441, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATCAAATGATTTCTTTGATAAATGAAGCTTTTGCTGGAGAAAAATCAGTCTTTTCTGGTGACGGTCAACTGCTTGCTTCTACAGAATCTCGCGTGGTAAGTCTGAATTTAACGGGTTTTATGGCGTATTCTGGTCAAGAATTTTGA", "aa": "MNQMISLINEAFAGEKSVFSGDGQLLASTESRVVSLNLTGFMAYSGQEF", "aa_hexdigest": "70180dd86d51c02dec26019dbff4d0a3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5276.881699999999, "isoelectric_point": 4.251295280456543 }, "id": "NKOCPLAKPK_1822", "locus": "GCA003260355_01813" }, { "type": "cds", "sequence": "contig_2", "start": 107481, "stop": 107714, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGACGAACCAGCTTTTTCTTTCTTTTCTTTCCCAATTTAGGGGAGCTGATATGGATTTTTATTTTTATCCGGAAAGGTTTCGTTTTTCTCAGGGAGAGGGGAATGTAGGCTCACAATTTGTTCAATTGATTGCGAAAGATCTCCTTCATCATTGGATAAAAATTCCATGGAATAAAGATGTTTTCGTATTTCCTTCTGAGGAATTACTTTTATTTCCACAGATTTTTTCTTGA", "aa": "MTNQLFLSFLSQFRGADMDFYFYPERFRFSQGEGNVGSQFVQLIAKDLLHHWIKIPWNKDVFVFPSEELLLFPQIFS", "aa_hexdigest": "80fa9639837510bc0d45ff7c83a8e2c3", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9210.462599999997, "isoelectric_point": 5.473106193542479 }, "id": "NKOCPLAKPK_1823", "locus": "GCA003260355_01814" }, { "type": "cds", "sequence": "contig_2", "start": 108306, "stop": 108821, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTATAATAAGCAGAATGCTATCGTAAAAGATGTATTACAGTTTATGGGCGATCAGGAGAAGCTAATTGAATCAAATCTTAAAGCACAGGTAGATGTGCAGACTGATGGAGGTAAAGGAAAAGTTATGCTTGATGTGAAGAGTGTATCTGATAATAAGACGAAAGATTCTGAGTCTGACATAGGTCTTAATCTTACTATGGATCAGAAAGATGCTCAAACAGGAGATCTTCAGAAATTGGATGCGAAAGTGAACTTGAATACGCTTTTGAAGGATTATCAGCTCTATTTTAAGCTTAATGAGTTTGTATTGGAGACAAATCCAAAAGATGCTGGGGGAATGCCGTTAGCGATGGTTGAAATGTTTAAGAATAAGTGGCTTACTTTGGATGTTCCAGAAATTAAGGAAATGCTTAGGACTTCAGCAGAGACTAATGCTAATATTTGGCAGAATGAAAAACTTTATGCAGTGAATCCAAGTTATTATACGGGAGTGGTATCAACTACTTATGAATGA", "aa": "MYNKQNAIVKDVLQFMGDQEKLIESNLKAQVDVQTDGGKGKVMLDVKSVSDNKTKDSESDIGLNLTMDQKDAQTGDLQKLDAKVNLNTLLKDYQLYFKLNEFVLETNPKDAGGMPLAMVEMFKNKWLTLDVPEIKEMLRTSAETNANIWQNEKLYAVNPSYYTGVVSTTYE", "aa_hexdigest": "788807227b2779aa598857b4efb366a4", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19342.768700000008, "isoelectric_point": 4.812182426452637 }, "id": "NKOCPLAKPK_1824", "locus": "GCA003260355_01815" }, { "type": "cds", "sequence": "contig_2", "start": 108870, "stop": 109157, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCTTGATTTCTATGCTTTGAATGAGACTGGAGATGTGGCTTCTACTGGAAATAAAGTATTGCTTGCTCAACAGCAGCAAATGAAAGACACTTTGGATCAGATTTTTTCTAGTATAAAATTTGAGAATACTGAAGCTTATTTTGTGATTCGTTCTGCAGATAAGGTGGATTTTGTAGTAGTAAATAGTGATTTCTCAATGGGTGATCTTAAAATGAAGCTTAGTCAGCAAGTAAAAGGTAAGGTAACGACAGCTAAGGCGATTATTCCATCTGCTAATGGGTCTTGA", "aa": "MLDFYALNETGDVASTGNKVLLAQQQQMKDTLDQIFSSIKFENTEAYFVIRSADKVDFVVVNSDFSMGDLKMKLSQQVKGKVTTAKAIIPSANGS", "aa_hexdigest": "beba15570dc73925ded5a5e26d814f7d", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10428.767699999991, "isoelectric_point": 5.194310951232908 }, "id": "NKOCPLAKPK_1825", "locus": "GCA003260355_01816" }, { "type": "cds", "sequence": "contig_2", "start": 109290, "stop": 109469, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAGTCAAAATCGTTTCTCTCTTCAACCTCAGTTTAAGTTGACTATAGATAAAGTGGGAGTAGATGTAGACGGAGATTTCGTTGCAAAGAAAATCGCTAATCATACTTTTACTGCGCCTACTGATGCTCAGAATCTTCAAGAAATTTGGAGTGCATTAATGGGAGGTGGAGCTATGTGA", "aa": "MSQNRFSLQPQFKLTIDKVGVDVDGDFVAKKIANHTFTAPTDAQNLQEIWSALMGGGAM", "aa_hexdigest": "51a69e27fead0498e4e22a658e6eaff1", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6440.2564, "isoelectric_point": 5.485894966125487 }, "id": "NKOCPLAKPK_1826", "locus": "GCA003260355_01817" }, { "type": "cds", "sequence": "contig_2", "start": 109427, "stop": 109645, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGATTCAGACTTCTTTTTTTGATCAATCTCTACAGAATTATCAAGCAGTTCCAGAAAGAGAAGTAGTTCCAGAAAGAGAAGTAGTTCCAGAAAGAGAAGTAGCTCCAGATAGAGATACAGCTCCAGATATTACATCTTCCCCAGAGAAATCATCAAACTCTCCTGAAAATCCTCACATAGCTCCACCTCCCATTAATGCACTCCAAATTTCTTGA", "aa": "MKIQTSFFDQSLQNYQAVPEREVVPEREVVPEREVAPDRDTAPDITSSPEKSSNSPENPHIAPPPINALQIS", "aa_hexdigest": "6152927c05bb37a3a3eb9b161333a1be", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8025.7527999999975, "isoelectric_point": 4.482516288757325 }, "id": "NKOCPLAKPK_1827", "locus": "GCA003260355_01818" }, { "type": "cds", "sequence": "contig_2", "start": 109732, "stop": 110142, "strand": "+", "frame": 1, "gene": null, "product": "ComEC/Rec2-related protein domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328ECX9", "UniRef:UniRef90_A0A660MT79" ], "nt": "ATGGATACTTTTGCTTTAAAGGTTATTTCTGGTAGTTTCCTCTTAGGAAAGTTGCCAGACCTTGGTCGGAGAATTGCTTCTTCTGGATGTGTTCTTTCTAGTGGAGTTTTTAATTTTTCCGAGATTAAGTGTGAAGAAAAATCGAAGTTTTCCGATCTCCTGGTGGTCGTTTCCAATCTCTTTTGTCTTAGAAAAGTTCCGATTAAATCAGACTTTTTGCGAAAGGTTTCTATGGCTCTTTTGGGAGAGCGAGTGATGATTATCCTTCCTGAGCGATGGTTTCAAGATGCGAAAACTCGATATATCGTTGCTGATGATCTGGGAAGTCTCTGGCTTTTGCATTCATTAACGATAGAAATGAGTAATGTGATCCAGCATCCTGTCTCATACTTTGTCGTTCCACAGGTATGA", "aa": "MDTFALKVISGSFLLGKLPDLGRRIASSGCVLSSGVFNFSEIKCEEKSKFSDLLVVVSNLFCLRKVPIKSDFLRKVSMALLGERVMIILPERWFQDAKTRYIVADDLGSLWLLHSLTIEMSNVIQHPVSYFVVPQV", "aa_hexdigest": "4ba938ab6420d6af8b59e5b778fe0c29", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 109732, "stop": 110358, "observations": { "mutation": [ 110140, 110206, 110218, 110254 ], "stop": [ 110140, 110206, 110218, 110254 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A660MT79", "query_cov": 1.0, "subject_cov": 0.6538461538461539, "identity": 0.89, "score": 236.0, "evalue": 2.81e-77, "gene_start": 1, "gene_end": 136, "reference_start": 1, "reference_end": 136, "reference_sequence": "MHTFALKVISGSFPIGKLPSLGRRIASSRCVLSSGVFNFPEVKYEEKSKFSDLLVVVSNLFCLGKVPIKSDFLRNVSMALLGEQVMIILPERWFQDAKTRYIVADDRRSLWLLHSLTMEMSNAIQHPVSYFVVPQVGDYCFEGIPSLILGVSFFCVCSGFSLGYLHSDVLFYLFGGNVFFDLRMFVVCFAAFLWLFFVLFWRKCPFSF" }, "paralog": false, "identity": 0.8894230769230769, "score": 365.0, "evalue": 1.11e-130, "description": "Internal stop codon at 110140, 110206, 110218, 110254. Nonsense mutation around 110140, 110206, 110218, 110254.", "psc": { "uniref90_id": "UniRef90_A0A660MT79", "product": "ComEC/Rec2-related protein domain-containing protein", "uniref50_id": "UniRef50_A0A328ECX9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A660MT79", "UniRef:UniRef50_A0A328ECX9" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328ECX9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328ECX9" ], "product": "ComEC/Rec2-related protein domain-containing protein" } }, "id": "NKOCPLAKPK_1828", "locus": "GCA003260355_01819" }, { "type": "cds", "sequence": "contig_2", "start": 110546, "stop": 110779, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTTTCTTTTTGGTGGATCGTTTCGCTCGTTGGGGCAGATCAGAATTCTTTTTCTTTTTCGAGATTTTTGTATCGATAGGCGAAGCTTTCTGCATAATCTTCTTCTTTGCTCTTTTGTGCGTATTCGCTGAAAAATCCTTTGGAATTGCAGGTATTTTGCTCGTTATTTCGGCAAATAGTATCGAAAGCTTGGCTATTTTGGTCCCTGAAAAAATAGATGTAATGTCCTAA", "aa": "MIFFLVDRFARWGRSEFFFFFEIFVSIGEAFCIIFFFALLCVFAEKSFGIAGILLVISANSIESLAILVPEKIDVMS", "aa_hexdigest": "29b264dab61c6881dcae0082ff052128", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8788.402699999993, "isoelectric_point": 4.601025581359862 }, "id": "NKOCPLAKPK_1829", "locus": "GCA003260355_01820" }, { "type": "cds", "sequence": "contig_2", "start": 111068, "stop": 111613, "strand": "-", "frame": 1, "gene": null, "product": "Peptidase M10 metallopeptidase domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EGY0", "UniRef:UniRef90_A0A328EGY0" ], "nt": "ATGAAAAAGAAATACCTATCTATTCTGCTCTTAGGAACATTACTTCTCCATTTCGGAACAGGGAAAGCAGAAAATTCAGATCAAAAATTAGATTCTATCTTCGAAATCCAAGACTACGTAGAACAAACACTTAATATCAAATCCTCAACAATCTCAAGCAGAAACTATTTCCCTCTTATCAAAGAAATCTTAGAGAAAAAGTGCCCCTCCGAGAGCATAGATGAAGCCAAAAGTACTCTTGAACAAACCCTCCCTACTCGACAAGAGCTAATAAGCCAGATTTCTCCTAATAGAATCAAGTATGAATTTGAAAAAATCAAAACCTCAACATTGCAAATCCAAACAGAAAACCCTACCGAATTTTGCAAAAAAAAATACATCAGTTATACCTTCCTAGAAATGATGAGAAATCAGAATCTTTCTCACTACCAAAATGAACAAGAACACCCAGCTGCCAACCCTAGACAATCGGAAAGAGAAAATTCCACTTCTCAAAACCAACAACCAACAATAGAAGTCCAAAACAATACAAAAAACCTCTCATGA", "aa": "MKKKYLSILLLGTLLLHFGTGKAENSDQKLDSIFEIQDYVEQTLNIKSSTISSRNYFPLIKEILEKKCPSESIDEAKSTLEQTLPTRQELISQISPNRIKYEFEKIKTSTLQIQTENPTEFCKKKYISYTFLEMMRNQNLSHYQNEQEHPAANPRQSERENSTSQNQQPTIEVQNNTKNLS", "aa_hexdigest": "af8ed3f0f17303ab296d224b33db0306", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 110765, "stop": 111613, "observations": { "mutation": [ 110935, 111070 ], "stop": [ 110935, 111070 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EGY0", "query_cov": 1.0, "subject_cov": 0.49725274725274726, "identity": 0.978, "score": 329.0, "evalue": 4.86e-111, "gene_start": 1, "gene_end": 181, "reference_start": 1, "reference_end": 181, "reference_sequence": "MKKKYLSILLLGTLLLDFGIGKAENSDQKLDSIFEIQDYVEQTLNIKSSTISSRNYFPLIKEILEKKCPSESVDEAKSTLEQTLPTRQELISQISPNRIKYEFEKIKTSTLQIQTENPTEFCKKKYISYTFLEMMRNQNLSHYQNEQEHPAANPRQSERENSASQNQQPTIEVQNNTKNLSGEDLTFAEQTKQFAHEELTNLLSLGILNPHDQETLNNHIEINYLPGCKKTHGSFHILQNKTTKEKKFKTLKLNIAICNTAEYQANYRAYFKQILAHELGHYIYFFRDQNSQAFDTICRNNEQNTCNSKGFFSEYAQKSKEEDYAESFAYRYKNLEKEKEFGSAPTSETIYQKENHFNQLFASH" }, "paralog": false, "identity": 0.9680851063829787, "score": 544.0, "evalue": 6.23e-198, "description": "Internal stop codon at 110935, 111070. Nonsense mutation around 110935, 111070.", "psc": { "uniref90_id": "UniRef90_A0A328EGY0", "product": "Peptidase M10 metallopeptidase domain-containing protein", "uniref50_id": "UniRef50_A0A328EGY0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EGY0", "UniRef:UniRef50_A0A328EGY0" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EGY0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EGY0" ], "product": "Peptidase M10 metallopeptidase domain-containing protein" } }, "id": "NKOCPLAKPK_1830", "locus": "GCA003260355_01821" }, { "type": "cds", "sequence": "contig_2", "start": 111867, "stop": 112394, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATAGACAAGAAAATGGTCAAAGGGTTGGCGATTATGTCGCTCGTTATGTTGCAGGCTAATATCTTTGTGCCAACAGGGATTTCACTCGCGGAGGAGGGTGAGTCTGTTGCTTCAACTGTGCAAGGAGAGAGCTTGGCAATTGAGGGTTCTTCTGTACAAGAGGATTTAGCTTCTGCTTCTGCAGAGGAGAGTGTAACGGGGACGGCAGCACAGGTTTTTGAAGAAGTAGTTCAAGCTACTGGAGAAGTGGTAGATGCTTCAGGAGTGGTGATGCCATCTCTTGATGTGTTGATGTCTGCAACAGGTGCTCATATGTCTGGTGAATTGTCTGCTGGAATAATTTCTAGCTTAGGAATGAATGTAGGTGGATCTACGCTTGTGAAATATTTTACTATTGGCTCTGATGATGAGATTCCTCTTTTGATGAAAGATGATGGAACGGTTGATTATACCGGTACTAAATTGCAATATTCTGATACAACGGATTATTATTTGAATACGAAGTCTCAAGTGGTTGATTTTTGA", "aa": "MIDKKMVKGLAIMSLVMLQANIFVPTGISLAEEGESVASTVQGESLAIEGSSVQEDLASASAEESVTGTAAQVFEEVVQATGEVVDASGVVMPSLDVLMSATGAHMSGELSAGIISSLGMNVGGSTLVKYFTIGSDDEIPLLMKDDGTVDYTGTKLQYSDTTDYYLNTKSQVVDF", "aa_hexdigest": "972971794a76fdddff96ae5c9edfc623", "start_type": "ATG", "rbs_motif": "TTATT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18206.295400000003, "isoelectric_point": 4.0500284194946286 }, "id": "NKOCPLAKPK_1831", "locus": "GCA003260355_01822" }, { "type": "cds", "sequence": "contig_2", "start": 112671, "stop": 112817, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGACTTTACAAGATGCGATAGCTTCTGCTAGTGATGGGGATACTATTCTTCTTACAAAGGATATTTCTGCTGGAAAGGCGTTGTATACGACAGCAGTTGGTGCTCATGTATGTACTACTGGAAGTTCTGAGCAAAAGACTACATGA", "aa": "MTLQDAIASASDGDTILLTKDISAGKALYTTAVGAHVCTTGSSEQKTT", "aa_hexdigest": "1e0235f868ad1db795535b44dddcef18", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4844.342900000001, "isoelectric_point": 4.74869327545166 }, "id": "NKOCPLAKPK_1832", "locus": "GCA003260355_01823" }, { "type": "cds", "sequence": "contig_2", "start": 112947, "stop": 113075, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGACCTCCAATAATGCTAATGTGCATATGATTATTCAAAATTATGCGAAGTTAACATTGGAAAACTTTACTTTGGATGCAGCGGATCTTGAGAATCTGCCTGTATATGCTATTAGTAATAACTTTTGA", "aa": "MTSNNANVHMIIQNYAKLTLENFTLDAADLENLPVYAISNNF", "aa_hexdigest": "e5ae55d322103e3fcb6b09dff42ee3df", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4729.2595, "isoelectric_point": 4.307679557800294 }, "id": "NKOCPLAKPK_1833", "locus": "GCA003260355_01824" }, { "type": "cds", "sequence": "contig_2", "start": 113148, "stop": 113312, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGCACTAATTATGATGGAGGAGTTTCTGTAAAGTTTGCTAGTGATTTTGTTGGAAGTGTAGATGGTGATATTGAATATGGTCCAGCTAATAGGGTACAAAGTACTCCTAACTGGGAAGAGAAGGCTGAGCTTTCTATTGAGGGAAATGGAAAATTTAATTGA", "aa": "MSTNYDGGVSVKFASDFVGSVDGDIEYGPANRVQSTPNWEEKAELSIEGNGKFN", "aa_hexdigest": "d2fb4bb821d7f622f53289571628bc74", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5815.1759999999995, "isoelectric_point": 4.207586097717285 }, "id": "NKOCPLAKPK_1834", "locus": "GCA003260355_01825" }, { "type": "cds", "sequence": "contig_2", "start": 113514, "stop": 113696, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGATGGGGAGACAATTAAGCTCAATGAAACTCATGCATTGATGAGAAAGGTGACAGGTGTGAGCTCTGAAATGACCACAGTATATAACAAGATCTTAGAATTTACTGATACCACAAATCTTCTTGAAATTAAGGAGGTAGCTGGAGAGAAGCTTTTGTTTGTAAATATGGCTACGGCTTGA", "aa": "MDGETIKLNETHALMRKVTGVSSEMTTVYNKILEFTDTTNLLEIKEVAGEKLLFVNMATA", "aa_hexdigest": "aa64b78bf8fed1e3ae28301c2a347f03", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6691.675999999998, "isoelectric_point": 4.910002326965332 }, "id": "NKOCPLAKPK_1835", "locus": "GCA003260355_01826" }, { "type": "cds", "sequence": "contig_2", "start": 113883, "stop": 114137, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF09479.16" ], "nt": "GTGATAGGATATACTCTTCAAACAGCTAAGCCTGATTATGGACGATTCTATGCACCAAAGAAATATAAAATTACCTTTAATGCTAATGGAGGAACTGCTGTAGCGCCAGTTGTACAAGGTTATGGAACTCCTGTTACAGCTCCTGCAAATCCTACAAGAAGTTGTCATAGATTTGCCGGATGGAGGCCAGCGCTTCCAGCGACTATGCCTGATGGAGATATTACTCTCACTGCTCAATGGGCCTATACTTGTTGA", "aa": "MIGYTLQTAKPDYGRFYAPKKYKITFNANGGTAVAPVVQGYGTPVTAPANPTRSCHRFAGWRPALPATMPDGDITLTAQWAYTC", "aa_hexdigest": "d15577ac38d31242049725c5bc0b5eb6", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF09479.16", "name": "Flg_new", "length": 67, "aa_cov": 0.6904761904761905, "hmm_cov": 0.9701492537313433, "evalue": 4.781962562684988e-13, "score": 45.533958435058594, "start": 22, "stop": 79 } ], "seq_stats": { "molecular_weight": 9084.2742, "isoelectric_point": 9.513486671447755 }, "id": "NKOCPLAKPK_1836", "locus": "GCA003260355_01827" }, { "type": "cds", "sequence": "contig_2", "start": 114125, "stop": 114232, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTATTTCCAGTATTTGAAGATGAAGAACTTGAGCTACTTGAAGAAGATGATGAACTACTTGATCCACCACCTCATCCTCCTCAACCTCCTCCTCAACAAGTATAG", "aa": "MLFPVFEDEELELLEEDDELLDPPPHPPQPPPQQV", "aa_hexdigest": "b7225eeaec4c12c94f5fb23aa6b45363", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4080.4778000000024, "isoelectric_point": 4.0500284194946286 }, "id": "NKOCPLAKPK_1837", "locus": "GCA003260355_01828" }, { "type": "cds", "sequence": "contig_2", "start": 114261, "stop": 114518, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTACTCAGGAGAGGTTACTTCTCCGCTTTGCTCTGTTGAAGGATCTCAGTTTTCAGATGAACAGAATCAAGCTTACCTTTGGGCATGTGGGAAAGATATTACGACTATTAGAGAGATTTCAGGTGCGAGACTTGATCAGCCACTTACCAGAGCAGAACTTGCAAAGATGATGTCAGTGTATGTGACTAAGATTTTAGGGAAACAGCCTGTTGTGACAGGAACTGTTCAGTATGCAGATGTTGATGAGAAATTGTGA", "aa": "MYSGEVTSPLCSVEGSQFSDEQNQAYLWACGKDITTIREISGARLDQPLTRAELAKMMSVYVTKILGKQPVVTGTVQYADVDEKL", "aa_hexdigest": "5dc9fef0fa582d4c31875dc8dc3dba70", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9361.553799999996, "isoelectric_point": 4.72709445953369 }, "id": "NKOCPLAKPK_1838", "locus": "GCA003260355_01829" }, { "type": "cds", "sequence": "contig_2", "start": 114561, "stop": 114728, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGAATTCATCATGATGGAACAGCATTGGCTCGTTTTGAACCACATAAGCTTGTTACGAGAGCTGAGTTTGCTACTGTGTTCTCTAGAGTACTTTATGGAAATAAGTATAACCAAGCTGGAGAAAACTGGGCAAAAGGTCATCTTGAAGCTCTGAAAAATGCTTGA", "aa": "MGIHHDGTALARFEPHKLVTRAEFATVFSRVLYGNKYNQAGENWAKGHLEALKNA", "aa_hexdigest": "7d39a828d6bb56e19307ee8f958de5e0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6166.898000000001, "isoelectric_point": 9.301514625549316 }, "id": "NKOCPLAKPK_1839", "locus": "GCA003260355_01830" }, { "type": "cds", "sequence": "contig_2", "start": 114929, "stop": 115132, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DC032CF", "UniRef:UniRef100_A0A328EGC0", "UniRef:UniRef50_A0A328EGC0", "UniRef:UniRef90_A0A328EGC0" ], "nt": "ATGGCAACAAAAGTTATAGATTTTGAAAACAAGTCGCTTTATGAGCTTAGGAAAGAGATAGTAGAATTTTTAAAAGAAAATCCAAAAATCAAGATTATAGAATCGGTCATGGACTATGAAATCCCATATAGTGTTAAGGGACCAGGGACACTATCCCATGAAGGACGTCATTGTATGATCTTATTTTATGAAGAGGGTGGATAA", "aa": "MATKVIDFENKSLYELRKEIVEFLKENPKIKIIESVMDYEIPYSVKGPGTLSHEGRHCMILFYEEGG", "aa_hexdigest": "b4ab3052b99abe3613ff0779aa157f2f", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000DC032CF", "uniref100_id": "UniRef100_A0A328EGC0", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DC032CF", "UniRef:UniRef100_A0A328EGC0" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EGC0", "uniref90_id": "UniRef90_A0A328EGC0" }, "psc": { "uniref90_id": "UniRef90_A0A328EGC0", "uniref50_id": "UniRef50_A0A328EGC0" }, "pscc": { "uniref50_id": "UniRef50_A0A328EGC0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EGC0" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7794.948700000001, "isoelectric_point": 5.2988378524780275 }, "id": "NKOCPLAKPK_1840", "locus": "GCA003260355_01831" }, { "type": "cds", "sequence": "contig_2", "start": 115344, "stop": 115523, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGAAAAGTAAAAAGCACTTGCAAACTCAAAACGAAATCCTATAGTCAGGATTGTTTTATTTTACTATATCGTAGCATATGTCAGAAACAAAAAAGTATGTCTACTCTTTTTCGGAAGGAAAGGGTGTTGGGAAAGAAATCCTCTGAGGAAAGGGAGCTAATCTCGCTGAAATGGTAA", "aa": "MRKVKSTCKLKTKSYSQDCFILLYRSICQKQKSMSTLFRKERVLGKKSSEERELISLKW", "aa_hexdigest": "ad04daec56dc6854559342fba55a96c9", "start_type": "ATG", "rbs_motif": "TTATT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7077.391399999997, "isoelectric_point": 10.08931827545166 }, "id": "NKOCPLAKPK_1841", "locus": "GCA003260355_01832" }, { "type": "cds", "sequence": "contig_2", "start": 115517, "stop": 117034, "strand": "+", "frame": 2, "gene": null, "product": "Pyruvate, phosphate dikinase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A355V105" ], "nt": "ATGGTAAGTTTAGGTTTGCCAATCCCTTCGGGATTTACTATCACGACGGAAACGTGTGATATTTACTACAAAAACAATAAAAGTTACCCAGCAGAAGTGGATCAACAGGTAGAAGAACAGCTTCAAGCGCTTGAGGCAAGAATGGGAAAGAAGCTTGGTGATGCGAATGATCCTTTGCTTGTATCTGTAAGATCTGGAGCTGCAGCTTCACTTCCAGGAATGATGGATACGGTTTTGAATCTTGGACTTAACGATGAATCTGTAGTAGGACTTGCTACCAAGACAGGAAATGAAAGGTTTGCTTGGGATTCGTATAGAAGATTTATTCAGATGTTTGGAGATGTGGTGATGGAAGTGCCTCATCATGACTTTGAAAAGGCCTTGCAATCTGTAAAAGATAAAAAAGGAGTAAAGCTGGATACAGAACTTGATACGGACGACCTCAAAGAAGTTGTAAGACTCTATAAAGAAGTCGTACAGAGAGATACAGGGAAACTTTTCCCAAATGATGGAAGACAACAGCTTAAAATGGCAATTGATGCGGTATTCAACTCATGGAATAATGAAAGAGCGATTATTTATAGAAGAATCAACGATATTAGAGGACTTCTTGGAACTGCGGTAAATGTGCAGTCTATGGTCTTTGGAAATATGGGAGAAACTTCAGGAACGGGAGTATGTTTTACAAGGAATCCTTCTACTGGGGAAAATAAATTTTACGGAGAATTTTTGATGAATGCTCAGGGAGAAGATGTGGTAGCTGGAATCAGAACTCCAGCACATATTGAGGAGCTTGATAAGGTAATGCCTGACTGCTACAAAGAGCTTTGTGATATTTATCACTTGCTGGAAAAGCATTATAAAGATATGCAAGACATGGAGTTTACGATCCAGGAAGGAAAACTTTATATTCTCCAGACAAGAAGTGGAAAAAGAACTGCTGCTGCGGCAGTGAAGATTGCTATGGATATGCTTGGAGAAGGACTTATCGATGAAAAAACGGCTGTACTTAGAGTGCAGCCAGAACAGCTTGATAGCTTGCTTCATCCTCAGCTTGATCCTCAGGCTAAAAAGGAAGCTGAACTTCTTGTGAAAGCACTTCCTGCTTCGCCAGGTGCAGCTGTGGGTCAGATCGTTTTCTCTGCTGATGTTGCTCATGATTGGAAAGAGAATGGAAAGAAAGTAGTGCTTGTGAGATTAGAAACTTCACCGGAAGATATTCAAGGAATGATTGCTTCTGAAGGAATCTTGACTGCGAGAGGAGGAATGACTTCTCATGCTGCGGTAGTTGCAAGAGGAATGGGAAAATGCTGTGTCTCTGGAGCTGGAGAGCTTGTTATTGATGAAGTTGCGAAGACGGTAACTGTTCATGGAAGGACTTTCAGAGAAGGGGATCAGATTTCTCTTGATGGAGGAACGGGAGAAGTGTTTGCTGGAGCATTAAAAGTAGTGGATCCAGAACTTAGTGGAGATTTTGAGAAATTAATGCAGTTGGCGGATAAATATAGAAAACTCTGA", "aa": "MVSLGLPIPSGFTITTETCDIYYKNNKSYPAEVDQQVEEQLQALEARMGKKLGDANDPLLVSVRSGAAASLPGMMDTVLNLGLNDESVVGLATKTGNERFAWDSYRRFIQMFGDVVMEVPHHDFEKALQSVKDKKGVKLDTELDTDDLKEVVRLYKEVVQRDTGKLFPNDGRQQLKMAIDAVFNSWNNERAIIYRRINDIRGLLGTAVNVQSMVFGNMGETSGTGVCFTRNPSTGENKFYGEFLMNAQGEDVVAGIRTPAHIEELDKVMPDCYKELCDIYHLLEKHYKDMQDMEFTIQEGKLYILQTRSGKRTAAAAVKIAMDMLGEGLIDEKTAVLRVQPEQLDSLLHPQLDPQAKKEAELLVKALPASPGAAVGQIVFSADVAHDWKENGKKVVLVRLETSPEDIQGMIASEGILTARGGMTSHAAVVARGMGKCCVSGAGELVIDEVAKTVTVHGRTFREGDQISLDGGTGEVFAGALKVVDPELSGDFEKLMQLADKYRKL", "aa_hexdigest": "cb4b0185ac13ed92a2c68bb145890e81", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI003217F98C", "query_cov": 1.0, "subject_cov": 0.8444816053511706, "identity": 0.612, "score": 610.0, "evalue": 1.29e-212, "valid": false, "gene": "ppdK", "product": "pyruvate, phosphate dikinase", "ec_ids": [ "2.7.9.1" ], "uniref50_id": "UniRef50_A0A355V105", "go_ids": [ "GO:0006090", "GO:0050242" ] }, "pscc": { "uniref50_id": "UniRef50_A0A355V105", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A355V105" ], "product": "Pyruvate, phosphate dikinase" }, "genes": [], "id": "NKOCPLAKPK_1842", "locus": "GCA003260355_01833" }, { "type": "cds", "sequence": "contig_2", "start": 117122, "stop": 117649, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02896.24" ], "nt": "ATGTTCTTTGCTGAGGAAAGAATTAAGGCAGTAAGAGAGATGATTCTTGCGGAAGATGTTGAGGGAAGAAAGAAGGCACTTGCAAAAATCCTTCCTTATCAGAAATCAGACTTTTATGAACTGATGAAAACTCTAGAAGGAATGCCAGTAACGATCAGATTTTTGGATCCTCCTTTGCATGAATTCCTTCCTCAAGAAGAAGCTCAGATTGCAGAACTTGCTACTGAACTTGGTGTGAGTGTAGAAAGACTTCATGAGAAAATAGCTTCTCTCCATGAATTTAATCCAATGCTCGGACATAGAGGATGTAGATTGGCAATTACCTTCCCTGAAATCGCAGAAATGCAGACTGAAGCTGTGATTTCTGCTGCTTGTGAACTCAAGAAAGAAGGAGTCGATGTTCATCCAGAAATTATGATTCCGCTCGTAGGATTCAAATCAGAATTAGATTTTAATGCTGACATCGTAAGAAAGGTAGCGAAAGCGACGATGGATAGATATGCTGTAGACGTGGAATACAAGCTCTGA", "aa": "MFFAEERIKAVREMILAEDVEGRKKALAKILPYQKSDFYELMKTLEGMPVTIRFLDPPLHEFLPQEEAQIAELATELGVSVERLHEKIASLHEFNPMLGHRGCRLAITFPEIAEMQTEAVISAACELKKEGVDVHPEIMIPLVGFKSELDFNADIVRKVAKATMDRYAVDVEYKL", "aa_hexdigest": "075d0729edaa2d45907cf420e122f435", "start_type": "ATG", "rbs_motif": "TTGTA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02896.24", "name": "PEP-utilizers_C", "length": 170, "aa_cov": 0.9657142857142857, "hmm_cov": 0.711764705882353, "evalue": 1.97712560790753e-21, "score": 72.87268829345703, "start": 1, "stop": 169 } ], "seq_stats": { "molecular_weight": 19918.016000000003, "isoelectric_point": 5.128264045715332 }, "id": "NKOCPLAKPK_1843", "locus": "GCA003260355_01834" }, { "type": "cds", "sequence": "contig_2", "start": 117653, "stop": 118051, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02896.24" ], "nt": "ATGATTGAGGTGCCAAGAGGAGCAGTAACTGCTGATAAAGTTGCAGAAACTGCGGAATTCTTCTCTTTTGGAACGAATGATCTTACTCAGATGGGACTTGGATTCTCTAGAGATGATGCAGGAAAATTTATCAAAGATTATGTAAATAAGAACATCTTTGAAAAAGATCCTTTCCAGACGCTTGATCAAGAAGGAGTAGGAGAACTTGTGAAGATCGCTGTAGAAAAAGGAAGAGCAACCAGATCAACTATCAAACTCGGAATCTGTGGAGAACACGGAGGAGATCCAGCTTCTATAGATTTCTGTCATAGAGTAGGTTTGACCTACGTAAGCTGTAGTCCGTATAGAGTGCCTATCGCAAGACTCGCTGCGGCACATGCTGCTTTGAAAAATGGATAA", "aa": "MIEVPRGAVTADKVAETAEFFSFGTNDLTQMGLGFSRDDAGKFIKDYVNKNIFEKDPFQTLDQEGVGELVKIAVEKGRATRSTIKLGICGEHGGDPASIDFCHRVGLTYVSCSPYRVPIARLAAAHAALKNG", "aa_hexdigest": "52c3878cd6289f4a167b37a54a2ad51e", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A1F3M1D6", "query_cov": 0.9772727272727273, "subject_cov": 1.0, "identity": 0.721, "score": 200.0, "evalue": 3.67e-64, "valid": false, "product": "Pyruvate, phosphate dikinase", "uniref50_id": "UniRef50_A0A7C4YMQ4" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02896.24", "name": "PEP-utilizers_C", "length": 129, "aa_cov": 0.9621212121212122, "hmm_cov": 0.8682170542635659, "evalue": 6.105785012168713e-34, "score": 113.93672943115234, "start": 1, "stop": 127 } ], "seq_stats": { "molecular_weight": 14237.022499999981, "isoelectric_point": 6.282095527648925 }, "id": "NKOCPLAKPK_1844", "locus": "GCA003260355_01835" }, { "type": "cds", "sequence": "contig_2", "start": 118563, "stop": 118688, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATCTTGGCTCTGATTCATTCTTTTGCACATACTATTAAGGGATTCTTTGTAGGAGTTGCGAAAGAGACTGTAAAAGTAGTAAGTCAGACTGTTGGGACGCCGATGAAGACAGATGCTTACTGA", "aa": "MILALIHSFAHTIKGFFVGVAKETVKVVSQTVGTPMKTDAY", "aa_hexdigest": "7d6086e1137a89eb038de94d6e72da71", "start_type": "GTG", "rbs_motif": "TTCTT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4437.229100000001, "isoelectric_point": 9.400538063049318 }, "id": "NKOCPLAKPK_1845", "locus": "GCA003260355_01836" }, { "type": "cds", "sequence": "contig_2", "start": 118692, "stop": 118910, "strand": "+", "frame": 3, "gene": null, "product": "Cell envelope-related transcriptional attenuator domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A948FPK8" ], "nt": "GTGAATGTTCTTTTGGTGGGATACGGAGGCGCTGCACATGGTGGAGGGTATTTGGCGGATTCGATCATTGTTGCTTCACGAAATCCAGAATTGGGTGCAGTGAGTATGCTTTCTATTCCAAGAGATCTTTATGTGAAGAATCCACTTGGAGGTTATTCAAGGATTAATGCCGTATTTACTCAGCTTTATGGTCGCACGAAGAGTCTTCCTGAGGCTTGA", "aa": "MNVLLVGYGGAAHGGGYLADSIIVASRNPELGAVSMLSIPRDLYVKNPLGGYSRINAVFTQLYGRTKSLPEA", "aa_hexdigest": "82399f45dd75548b2fab8bae056dfa9a", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A948FPK8", "query_cov": 0.8611111111111112, "subject_cov": 0.96875, "identity": 0.597, "score": 75.9, "evalue": 8.11e-17, "valid": false, "product": "Cell envelope-related transcriptional attenuator domain-containing protein", "uniref50_id": "UniRef50_A0A948FPK8" }, "pscc": { "uniref50_id": "UniRef50_A0A948FPK8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A948FPK8" ], "product": "Cell envelope-related transcriptional attenuator domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_1846", "locus": "GCA003260355_01837" }, { "type": "cds", "sequence": "contig_2", "start": 119229, "stop": 119372, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTTCAGGAGGAAACCTTAGCTTGGAAAAGGCAAAACAGCTCTATTCTGGTTATGTAGCAATGGTTAACACTAATGTTTCACTCAATGAGATGCTTTGGACAATTCAGTATTTAGATAGAGTAAAGCCGTTTTCTTTCTGA", "aa": "MLSGGNLSLEKAKQLYSGYVAMVNTNVSLNEMLWTIQYLDRVKPFSF", "aa_hexdigest": "0108f7c2c4da67531466c2f117f7de22", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5358.1712, "isoelectric_point": 8.098276710510255 }, "id": "NKOCPLAKPK_1847", "locus": "GCA003260355_01838" }, { "type": "cds", "sequence": "contig_2", "start": 119445, "stop": 119879, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTGGTGGGGCTGCGGTCTTGCTTCCTCTTGGGGCTACGGTTAATAATGTTACTTATTATAAGGCGATTCATGAGTATGTGGATTTTATTTCCCATCTTCAGAGATTTCTCCTAGAGAATGCGAAAATTCAAGTCGATAATGGTATTGATAAAGAACTTCTTCGTTCAAATAAGATGCAAAATGTGAGGGTGGCCAGTAGAGTTGCTTCTAAGATGAAAAGGTATGGATTGGATGTGATTGCTACTGACAACACAGATCCTCTTCCACTTACGAGGCTGATTATCAATACTGAAAATGCTGAGGCAAAAGGGTTTGAAGGAACTATTACAGCGATGAGGAACTTTGTGCCGATAGATGAGGTTGTGTATAATACAGGAGTGGTGAAGAAAATCATCGACGACTATGGGAATGAAGTAGAAGTGTTTACTTGA", "aa": "MFGGAAVLLPLGATVNNVTYYKAIHEYVDFISHLQRFLLENAKIQVDNGIDKELLRSNKMQNVRVASRVASKMKRYGLDVIATDNTDPLPLTRLIINTENAEAKGFEGTITAMRNFVPIDEVVYNTGVVKKIIDDYGNEVEVFT", "aa_hexdigest": "1e9f91d5bc28a94d8579ed4c4c2ff9fc", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16109.290299999982, "isoelectric_point": 5.67994327545166 }, "id": "NKOCPLAKPK_1848", "locus": "GCA003260355_01839" }, { "type": "cds", "sequence": "contig_2", "start": 120119, "stop": 120322, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCATAAGTCTTATGCGGCAGATTTTAAAGTGGCATATGATCATAATGAAAGACTGGAATTTGTCGGGGATGCTATGCTGGAATCGGTGATTGCGAAGAGGCTTTTCTTGGATTTCCCTGAGATGCCGGAATCTACGATGACACTTTATAAGATTGCTCTCGTAAGGGAGGAAACCCTCTGTAAAGTAGCGAAAAAAATCTGA", "aa": "MHKSYAADFKVAYDHNERLEFVGDAMLESVIAKRLFLDFPEMPESTMTLYKIALVREETLCKVAKKI", "aa_hexdigest": "7505e996e68188420cb284ee3a64cf4f", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7783.050599999995, "isoelectric_point": 6.066789436340332 }, "id": "NKOCPLAKPK_1849", "locus": "GCA003260355_01840" }, { "type": "cds", "sequence": "contig_2", "start": 120401, "stop": 120607, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAGCATTGATTGGATATCTAGCACTTGATATGGGCTATCCTATTATGGAGCAATTTGTGCTTGATAGTATCTATCCTGAGATTAATGAGGTTTCCAAAAGTCCCGTAAAATCTTATAAGACGATGGCACAGGAGCATATTCAAAAATTCTATAAACACTTGCCTGTTTATAATGATTATGATGAGAAGGTTGATGAAAAGTGA", "aa": "MEALIGYLALDMGYPIMEQFVLDSIYPEINEVSKSPVKSYKTMAQEHIQKFYKHLPVYNDYDEKVDEK", "aa_hexdigest": "140fedbc0ae84b86c7c3525dde07a2a1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8035.113000000002, "isoelectric_point": 4.770178413391113 }, "id": "NKOCPLAKPK_1850", "locus": "GCA003260355_01841" }, { "type": "cds", "sequence": "contig_2", "start": 120750, "stop": 120896, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACTGATCTTAAAGATCAGTTTTTTTGTTTTGTCAATATTTTCTTTTTCTTTTGTGGTGTTTTCGGTGGACTTTGTCGCTTGTTTTTGTGTTGTAAAAGGTGGCTTTATGATTGGCTTTATTGTGTTTTTGTGAGAAAAAAATAA", "aa": "MTDLKDQFFCFVNIFFFFCGVFGGLCRLFLCCKRWLYDWLYCVFVRKK", "aa_hexdigest": "fbd23c715c5664295e12e5fa7dc87231", "start_type": "ATG", "rbs_motif": "TTTA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5968.1982, "isoelectric_point": 8.879891395568851 }, "id": "NKOCPLAKPK_1851", "locus": "GCA003260355_01842" }, { "type": "cds", "sequence": "contig_2", "start": 121342, "stop": 124410, "strand": "+", "frame": 1, "gene": null, "product": "SLH domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EFH7", "UniRef:UniRef90_A0A328EFH7" ], "nt": "ATGAACGTTAAAAAAATGTTCGCAGGCTTGACTGGAGGGTTAATGCTTGCATCAGTTCTTCCTGTATCAGTACTTGGTGCTGCTTCATACAGTCAGGAACTACAAGAAGCCTACAATTACGCTTATTCAAAGGGTATTACTACTATGAGCCCTATTGATAATGCTAATATGTACGGTGAACTTACTAGAGGTCAGTTGGCAAAAATGATCGCTAACTGGGCTGAAAAAGAAATGGGTACTAAAGTTGACACAACTGCTGTTTGCTCTTTCTCAGATGCACACACAGCTGAAGGAGACTTGGCTACTTATGTAAAGAAAGCTTGTCAGATGGGATTGATGGGTCAAGGAATTGACAAATTCAGACCAAATGATAAAGTAACAAGAGGTGAATTTGGAACAACTCTTTCTAGAGCTCTTTGGGGAGACAAGTACAATGGAGCTACTCCATTCTACAAGAATCATCTTGAGGCTTTGAGAGATGCTGGAATCATGAAAATGATTGATACTCCAAATCAAATGGAAATTAGAGGATATGTAATGCTTATGCTTATGAGATCAGTTAATGTAGTTACTCCTTCTGAATGTAAGGATCCTGCTGTGATCTTGGCTTGTGCATTAGGTGGTGATGCTTGTCCTGCTAGATGTAAGGGTAAGACACCAGATACTACTAATACAGGAACTAATAATACTGCTGGAGATTTGAATATCAACGTAGTTGATTACTCAAACCAGATTAGGTCTGTTCCTATGGTTGGTATTTCTATCTTCAACGCTATCAAATTTGATGCTACTAATGATGTAACTGTTAATTCAGTTACTTTGGAAAGAGTTGGTCTTTCTAGTAGATCAGACATCAAAGGTGTATGGTTCGAAAAGGATGGTATCGCTGTATCATCTAGAGGAACTGTAGCTACAGATGGAAAAGTTATGACTAACTTCAACAAAGGTTTCAAGGTTAAGTCATCAGAAACGCTTGATCTAGTTGTAGAACTTAGCGGAGCTAGTGCAGGTGCAGAAGTTGCATTCAAGTTTATTGGTGTAGATTCTACTGCTAAAAATGCTGTTTACAACGCTGTAACTTCAACTTATAGAACTGCTAAATATGATGTAGCTGCTCTTGATTTCTCTAATGTTGGAGGAACTGGTAATGTTGTTTACAAGCTTGGAAATCAATCAGAATTGACTTTTGGACAATTCAAGGTTTCAAATCAAGCTAAGGGAGACGATAGAAATGTATTCTTGAAATCTGTTACTTTCAGAAACAACGGAACAGCAGATCTTAACTCTCTTCTTAAGAATGTAAAGGTTATGAGAGATAATAAAGTAGTTTCTAAGTCTGTATCTATGGATGGAAGAAACATTACTATTACTCTTGAAGATACTATCAACGCAGGAAAAGCAGTTGTTTATACTGTAATGGGTGAAGTAGCTTCTCTAGAAAGAGTTGGTGATACTGTTCAACTTGAACTTAGAAAGGATAGAGACTTGGTAGCTTATGAAGCTTCAACTAAGTTTAGAACTACTATGAATAAGCCTATTCAGGATAATTCATGGATCATGAAGAATTACAAGATCGATGGTGGTAGAGTTACTCTTACAAACACTGCTGCATTCCCTAAAACAGTTGATGCAGGAAGTGGATCTTCAGATGTTGTAATCGCAGATGGTACTCTTACTGTTGCTGAACCAATCAAGCTTCCTAAGATGACTCTTGCTGTAAATAATACAGGTGTTGTTAGATCTCTTGTTCTTGAAATCGGAGGATCTAGATATGCATCTACTGTAAATGGAACAGCTTTCGTATTTGATGATGTATATGTAAATAAGACAGCTTCTATTAAGCTTATCGCTTCATTAGAATCTTTGCCTGCACATAACTCAGTTCAGTTTGTTCCAACAACAATCGGATCATTGGCTTTCGGTTCTGCAAAGGGTGAATATACTAATAACTCAGAAGATTTGAAAGGAAATGATATCGCTGGTGCAGTACAAATTGCTAAGATTAATGTAAAAGAAGCTCAATTCACTCTTAAGAATAAAGCCTCTTCAACACAGAAAGTAACATTGACTGAATCTTCTATCAAGACAATCTTTGAAGGAGAAATGGTTGCTCCTAAGGGAGATATTAATGTAACTGAGGTAACTCTTTCTGGACTTAATGCTACTGGAATTACTGATAGTTCACAAATTGATCTTCAGCTCTATATCAATGATACTCCTGTATCTAATGCAACTTATAGAAGAGGAAATAATGTTGTAACATTCTACAATGTAGGAACTGCAACAAAAACTCCTTCTAAGTTTAGAATTGATGCTACTCCTACAATGACTCAGACTGGAGACTTCGTATTTGACGTAGTTGCTAAAGGAACTGATACTAACGGAAATACTGCAACAACTGCAGCTGTAAAGACTGTTAAATTGTCTGTAGTAGAATCTTCAAATGTATCTGTAGCTAATGCTAATGCTGTTAGCCAGGTAGTGAATGAAGGATCAAATGCTGAATTGACAAAGTTCACATTGTCTGCTAGAAATGGTTCAATCAACCTTACAGGTCTTAAGTTGAACTATAGTGGTTCCGTTGCTGCATTGAATGGACAGTCTGTGACTGTTGAAGTAAATGGAAATAGCTATACTGCTACTTCAACTGCTGCTGGTGTTGAATTCACTGGTCTTAACGTTACTCTTAACGAAGGAAATCATAATGTAGTATTCACAACGAATATTAACACTGATGGTCAACCTAATGGTGTTGCTGTTGAAGTTGCATCGGCAGAAGTGAGAGGAGACTTCCCAACAGTTACTAAGGCTATTAACTCTAAGTACTTGTTCGTAAAGGCATTCCCTAAATTGTCTGTAGTAAGTGCTAAGGATAATCAGTTGGTAGTTAAAATTGAAAATGTATCTGATGATGATATTACTATCACAGATTACGTTGCTGTTAATGCTGATCCTACGTCAACAACTCTTAATGGTCAGTCTGTTACTAGTCCAATTCCTGTAGGAAAGCAGGTTACTCTTGCTAAGGGACAATCAACTGAGCTTAAGATGAATATTACTGCAGCTTGA", "aa": "MNVKKMFAGLTGGLMLASVLPVSVLGAASYSQELQEAYNYAYSKGITTMSPIDNANMYGELTRGQLAKMIANWAEKEMGTKVDTTAVCSFSDAHTAEGDLATYVKKACQMGLMGQGIDKFRPNDKVTRGEFGTTLSRALWGDKYNGATPFYKNHLEALRDAGIMKMIDTPNQMEIRGYVMLMLMRSVNVVTPSECKDPAVILACALGGDACPARCKGKTPDTTNTGTNNTAGDLNINVVDYSNQIRSVPMVGISIFNAIKFDATNDVTVNSVTLERVGLSSRSDIKGVWFEKDGIAVSSRGTVATDGKVMTNFNKGFKVKSSETLDLVVELSGASAGAEVAFKFIGVDSTAKNAVYNAVTSTYRTAKYDVAALDFSNVGGTGNVVYKLGNQSELTFGQFKVSNQAKGDDRNVFLKSVTFRNNGTADLNSLLKNVKVMRDNKVVSKSVSMDGRNITITLEDTINAGKAVVYTVMGEVASLERVGDTVQLELRKDRDLVAYEASTKFRTTMNKPIQDNSWIMKNYKIDGGRVTLTNTAAFPKTVDAGSGSSDVVIADGTLTVAEPIKLPKMTLAVNNTGVVRSLVLEIGGSRYASTVNGTAFVFDDVYVNKTASIKLIASLESLPAHNSVQFVPTTIGSLAFGSAKGEYTNNSEDLKGNDIAGAVQIAKINVKEAQFTLKNKASSTQKVTLTESSIKTIFEGEMVAPKGDINVTEVTLSGLNATGITDSSQIDLQLYINDTPVSNATYRRGNNVVTFYNVGTATKTPSKFRIDATPTMTQTGDFVFDVVAKGTDTNGNTATTAAVKTVKLSVVESSNVSVANANAVSQVVNEGSNAELTKFTLSARNGSINLTGLKLNYSGSVAALNGQSVTVEVNGNSYTATSTAAGVEFTGLNVTLNEGNHNVVFTTNINTDGQPNGVAVEVASAEVRGDFPTVTKAINSKYLFVKAFPKLSVVSAKDNQLVVKIENVSDDDITITDYVAVNADPTSTTLNGQSVTSPIPVGKQVTLAKGQSTELKMNITAA", "aa_hexdigest": "e5994a532162cfacbf8d512241740734", "start_type": "ATG", "rbs_motif": "TTTA", "psc": { "uniref90_id": "UniRef90_A0A328EFH7", "query_cov": 1.0, "subject_cov": 0.9614299153339605, "identity": 0.993, "score": 1923.0, "evalue": 0.0, "valid": true, "product": "SLH domain-containing protein", "uniref50_id": "UniRef50_A0A328EFH7" }, "pscc": { "uniref50_id": "UniRef50_A0A328EFH7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EFH7" ], "product": "SLH domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_1852", "locus": "GCA003260355_01843" }, { "type": "cds", "sequence": "contig_2", "start": 124795, "stop": 124971, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTTTGGAGATGTTGGATGAGGCTTTTGATTGGGAAGACATGACCACCAGTTCCTCATCCTGCTCGTGCTATATTGGGAAATTTCATAGAATGAATCATATAGTAAGTAAAAAAAGAGAACAGCCACATGCAAAATCAATCAAAACCATCCGAATTAAAGCAAAAAAGGCATAA", "aa": "MSLEMLDEAFDWEDMTTSSSSCSCYIGKFHRMNHIVSKKREQPHAKSIKTIRIKAKKA", "aa_hexdigest": "cc0934e138cc91243135616f44e00667", "start_type": "ATG", "rbs_motif": "TTTTT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6731.7616, "isoelectric_point": 9.342774391174316 }, "id": "NKOCPLAKPK_1853", "locus": "GCA003260355_01844" }, { "type": "cds", "sequence": "contig_2", "start": 125527, "stop": 125817, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF04101.22" ], "nt": "TTGAAGACAACTCCAGAACTTGCAGAAATGCACTTCACGATCATTCTCGGGAAACTCAACCAAGACCTCGCTCAACTTTTTTCTGATTTTCCAAATGTGGAGACCTTGGCTTTTGTGAGTCAAAAAGAGATCGGTCGCCTCTATCTTCAGTCCGATATTGCGATCACGAGAGCAGGGACGACTTCACTTGCAGAGCAGGAACTTTTTGATCTAAAATTATTGATGATTCCAATTCCACGAACCCACGACCAGCTAGACAATGCGGAGCGATATGTTGAGCATAAAGACTGA", "aa": "MKTTPELAEMHFTIILGKLNQDLAQLFSDFPNVETLAFVSQKEIGRLYLQSDIAITRAGTTSLAEQELFDLKLLMIPIPRTHDQLDNAERYVEHKD", "aa_hexdigest": "b47dc9e56cd17406b944aa0021a5b7db", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF04101.22", "name": "Glyco_tran_28_C", "length": 91, "aa_cov": 0.8958333333333334, "hmm_cov": 0.967032967032967, "evalue": 2.17742123679378e-10, "score": 37.2855339050293, "start": 9, "stop": 94 } ], "seq_stats": { "molecular_weight": 10988.45069999999, "isoelectric_point": 4.905057334899902 }, "id": "NKOCPLAKPK_1854", "locus": "GCA003260355_01845" }, { "type": "cds", "sequence": "contig_2", "start": 126579, "stop": 127262, "strand": "+", "frame": 3, "gene": null, "product": "Mur ligase central domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A839JVJ0", "UniRef:UniRef90_A0A328EF08" ], "nt": "ATGAAGATTTGGAATTATTTTGAATTTCTCGGAGAAGTCACGAAATACTTTCGCACAGTCGGTTTTGCGGGGACAAATGGGAAATCAAGCTCTACCTCTTTAGCGATTGTTACAGGGAAGAAACTGATTCCTGAGCTGGGACTTGGAATTGTAGGAGCGCTAGTTCCTGATCTGAATAATAAGAGTTATTACCTAAAGGAAGATAAAAAATCTGAATTTAGCCAGCTTTTTGACTTTATTTTCACAGGAAAAAAACTCCCGTATGAACTGATCAAAAAATACTATTTCTTCTTGGAAGCCTGCGAATACAAGAGGCATTTTCTCAATCTTGACCTAGAATATCTTCTGATTACCAATATTGAGCTGGATCATACCGATTATTATCGTGATTTGGCAGATTATCAGTCTGCTTTCGTGCAGATGAATCAAAAAACCAAAAATCTGACTTTCGTTTTGGATTCCTTTGAGTCTCCAGAAATTTTTTCTAAAATAAAGAAAATTCCGCTCAAGCATTGGGAATTAGACCATATCCGAGGGAAACCGACCGATTGCAATGCTTCACTGGTCGCAGGACTTTTTCAGGAACTTAATCCTGAACTTTCTTTGGAGCAGATTGAAAAGGAAATGAAGCCATTCAAAGGAATTTGGAGGAGAATGGAGTTTCTAACGACACTTCCAAGCTGA", "aa": "MKIWNYFEFLGEVTKYFRTVGFAGTNGKSSSTSLAIVTGKKLIPELGLGIVGALVPDLNNKSYYLKEDKKSEFSQLFDFIFTGKKLPYELIKKYYFFLEACEYKRHFLNLDLEYLLITNIELDHTDYYRDLADYQSAFVQMNQKTKNLTFVLDSFESPEIFSKIKKIPLKHWELDHIRGKPTDCNASLVAGLFQELNPELSLEQIEKEMKPFKGIWRRMEFLTTLPS", "aa_hexdigest": "49ab95143f96c9f16c2157780f7fd790", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 126312, "stop": 127565, "observations": { "mutation": [ 126330, 126345, 127260, 127536 ], "stop": [ 126330, 126345, 127260, 127536 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EF08", "query_cov": 1.0, "subject_cov": 0.49455337690631807, "identity": 0.956, "score": 443.0, "evalue": 1.17e-153, "gene_start": 1, "gene_end": 227, "reference_start": 90, "reference_end": 316, "reference_sequence": "MHIVILGAGGSGISNIAHLLRDLGYTNLIAIDAQESQITQALREREIPVIIGHGKYQPKADDILIYSEATKESPEVQFAFQLKREEHFPMKIWNYFEFLGEITKYFRTVGFAGTNGKSSSTSLAIVTGKKLIPELGLGIVGALVPDLNNKSYYLKEDKKPEFRQLFDFIFTGKKLPYELIKKYYFFLEACEYKRHFLNLDLEYLLITNIELDHTDYYRDLADYQSAFIQMNQKIKNLTFVLDSFEAPEIFSKIKQIPLKHWNLDYIRGKPTDCNASLVAGLFQELNPELSLEQIEKEMKSFKGIWRRMEFLTTLPSGAKLFSDYGHIASSIQLAYETLRNRFPDHKLLAIFQPHQIHRVLKGRADFQKALAPFDQRAIFSIYTAREKFSDFADEPLFQEQHFQSFSEFGAYFAKAVKAEYLDTFQQVQELLATPDEKTIVVVFTAGDLDYQLRKSFLLG" }, "paralog": false, "identity": 0.947242206235012, "score": 792.0, "evalue": 7.88e-294, "description": "Internal stop codon at 126330, 126345, 127260, 127536. Nonsense mutation around 126330, 126345, 127260, 127536.", "psc": { "uniref90_id": "UniRef90_A0A328EF08", "product": "Mur ligase central domain-containing protein", "uniref50_id": "UniRef50_A0A839JVJ0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EF08", "UniRef:UniRef50_A0A839JVJ0" ] }, "pscc": { "uniref50_id": "UniRef50_A0A839JVJ0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A839JVJ0" ], "product": "Mur ligase central domain-containing protein" } }, "id": "NKOCPLAKPK_1855", "locus": "GCA003260355_01846" }, { "type": "cds", "sequence": "contig_2", "start": 127557, "stop": 127691, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAAAGCTGAATATCTGGATACCTTTCAGCAAGTGCAAGAACTCTTGGCTACCCCTGATGAAAAAACGATTGTCGTCGTATTTACAGCAGGGGATTTGGACTATCAACTGAGGAAAGCTTTTTTGCCTGGTTAA", "aa": "MKAEYLDTFQQVQELLATPDEKTIVVVFTAGDLDYQLRKAFLPG", "aa_hexdigest": "b69e52502a3d68ed3ff3f13ff5b6a327", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5003.6775, "isoelectric_point": 4.385492134094237 }, "id": "NKOCPLAKPK_1856", "locus": "GCA003260355_01847" }, { "type": "cds", "sequence": "contig_2", "start": 127985, "stop": 129232, "strand": "-", "frame": 1, "gene": null, "product": "DUF1015 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBFCA4A", "UniRef:UniRef100_A0A328EGY7", "UniRef:UniRef50_G5H964", "UniRef:UniRef90_A0A328EGY7" ], "nt": "ATGGTAGTAGTAAGACCATTCAAAGGGCTTCGCCCTCCACAAGAGATCGTAGCACAGCTTGCATCTCGCCCTTATGATGTGATGAATTCAGCCGAAGCGAGACAAGAAGCTGCAGGGAATCAGTATTCTCTTCTTCATGTTACCAAGGCAGAAATTGATCTTCCAGAAGGGACTGATGAGCATTCGCAAGCCGTATATGACAAGGTCGTAGAGAATTTCAAAGCATTCCAGGATAAAGGATGGCTTGTTCAAGATGCTGAACCAAAAATCTATATCTATGGGCAGACGATGGGTGAAAGAACCCAATACGGATTCGTAGCCTGCACCAGCATTGATGATTATTTCAATGAGAGCATCAAAAAGCACGAACTCACTCGTAGAGACAAGGAGGATGATAGAATGATCCATGTGAACATTACAGATGCAAATGTAGAACCGGTGTTTTTTGCCTATCCAGCACAGGCAGAGCTTGATGGAATCATTGAAGAAGTCGTAAAAAATCAGAAACCTGAATATGATTTCGTGGCAGATGATGGATTCGGACACCATTTCCGAGTGATCAATGATCAGGCTTTGGTTGCTAAAATTACCGAGCTTTTTGCGAAAGTTCCTGCGCTCTATGTTGCTGATGGACACCACAGAACCGCTGCTGCTGCAAGGGTTGGACTTGAGAGAAGAGCAGAGAAACCTGATTACACTGGAGAGGAAGAATTTAACTACTTTATGGCAGTAATTTTCCCAGATAATCAGCTCAAAATCATGGATTACAATAGAGTTTTGAAAGATCTCAACGGAATGAGCGAAGCAGAACTGCTTGAAAAACTTCAGACTCATTTCACGGTAGAAGAAAAAGGAGAAGCTGAATATAAACCTACAGCACTTCATAATTTCAGTATGTATCTAGGCAATAAACGATACTCTCTCACTGCGAAAGAAGGAACCTATGATGATAAAGATCCTATTGGAGTGCTTGATGTGACGATCCTTTCCAATCTCGTTTTGGATCAGCTCTTCGGAATCAAAGACCTCAGAACCGACAAGAGAATTGACTTTGTAGGAGGACTTAGAGGGCTCGGAGAGCTCAAAAGAAGAGTAGATAACGGAGAGATGAAAGTTGCTTTTGCACTCTATCCAGTAAGTATGCAGCAGCTGATTGATATTGCTGACACTGGAAATATCATGCCACCAAAGACAACTTGGTTTGAGCCAAAACTCAGAAGTGGACTCATTATTCATAAGTTATCATAA", "aa": "MVVVRPFKGLRPPQEIVAQLASRPYDVMNSAEARQEAAGNQYSLLHVTKAEIDLPEGTDEHSQAVYDKVVENFKAFQDKGWLVQDAEPKIYIYGQTMGERTQYGFVACTSIDDYFNESIKKHELTRRDKEDDRMIHVNITDANVEPVFFAYPAQAELDGIIEEVVKNQKPEYDFVADDGFGHHFRVINDQALVAKITELFAKVPALYVADGHHRTAAAARVGLERRAEKPDYTGEEEFNYFMAVIFPDNQLKIMDYNRVLKDLNGMSEAELLEKLQTHFTVEEKGEAEYKPTALHNFSMYLGNKRYSLTAKEGTYDDKDPIGVLDVTILSNLVLDQLFGIKDLRTDKRIDFVGGLRGLGELKRRVDNGEMKVAFALYPVSMQQLIDIADTGNIMPPKTTWFEPKLRSGLIIHKLS", "aa_hexdigest": "68bf35437b3f197308b13dfeeac8d289", "start_type": "ATG", "rbs_motif": "TTCTT", "ups": { "uniparc_id": "UPI000DBFCA4A", "uniref100_id": "UniRef100_A0A328EGY7", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBFCA4A", "UniRef:UniRef100_A0A328EGY7" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EGY7", "uniref90_id": "UniRef90_A0A328EGY7" }, "psc": { "uniref90_id": "UniRef90_A0A328EGY7", "product": "DUF1015 domain-containing protein", "uniref50_id": "UniRef50_G5H964" }, "pscc": { "uniref50_id": "UniRef50_G5H964", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_G5H964" ], "product": "DUF1015 domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_1857", "locus": "GCA003260355_01848" }, { "type": "cds", "sequence": "contig_2", "start": 129397, "stop": 129567, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTCGAAAGAAAAATCATTTATCCAAAAGATGTCGCTGAATATTACCATGAATTTCAAGACCAAAAAGGAAGTTTCATCACGTTAATGGGATGTTATCCTATCGGAAGCAATGCCCAGAGGATCATGGTGGTTGCTAAAAAAATCAATAACGAAGATGCAAACGCATAA", "aa": "MLERKIIYPKDVAEYYHEFQDQKGSFITLMGCYPIGSNAQRIMVVAKKINNEDANA", "aa_hexdigest": "f8819fbc5dd7f25aaa33756ebb75c8c3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6453.361100000002, "isoelectric_point": 6.534517478942871 }, "id": "NKOCPLAKPK_1858", "locus": "GCA003260355_01849" }, { "type": "cds", "sequence": "contig_2", "start": 129715, "stop": 130332, "strand": "-", "frame": 2, "gene": null, "product": "Sortase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328ECY1", "UniRef:UniRef90_A0A328ECY1" ], "nt": "ATGTATGTCCATCTTGATGAATTAGAAAACCTTTACGCAAATACCAGCGGAGAGCTCAGCATAGCTGAGTCTGACTTGGAGGCTAAGAGACCAGCCAAGAAATCATTCTCTTTTCCGGATTTCAAGCTCCCAAAAATGAAACTCCTGTCAGAGTTAAAAATACTGATTATCCTTTTCATCATTGTTTTCTCAGGATTTTTCTTTTTCACCAACGCAAGACTCGTCCTCTTAACAATCAACGATACCTTTGCGACCGAACCAAAAGCAGCTCCCGAGGATTTAAAAATAACCTCAGTCCCAACATCTCAAGATCACATTAAGCAAAAAGCACAAAAGCTGAATGAACTTGAAGATCAATTTACGAGCCTCCAGGCAAGCTTTAGAACAGAGAAAGAACTTGCTCCTACGATGAAAGAGTTCCTTGCAAAAAAGCAAGAAACCCACAGTATTGAATTTAATACCCTCCCTCCGACAAATAGACTCATCATTCCCGACCTCAACGTCAATACACCACTTATTGATGTCCCAATCAAAGGAGAAGAGGACTTTTCAGAGGGACAGTTTGATGAGGAATTGATGCAAGGTGCCGTAAAATACCCGACTACACCGGCTCCATGA", "aa": "MYVHLDELENLYANTSGELSIAESDLEAKRPAKKSFSFPDFKLPKMKLLSELKILIILFIIVFSGFFFFTNARLVLLTINDTFATEPKAAPEDLKITSVPTSQDHIKQKAQKLNELEDQFTSLQASFRTEKELAPTMKEFLAKKQETHSIEFNTLPPTNRLIIPDLNVNTPLIDVPIKGEEDFSEGQFDEELMQGAVKYPTTPAP", "aa_hexdigest": "2622a307e528a43ed66e540e585bdbf9", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 129412, "stop": 130332, "observations": { "mutation": [ 129717 ], "stop": [ 129717 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328ECY1", "query_cov": 1.0, "subject_cov": 0.6591639871382636, "identity": 1.0, "score": 385.0, "evalue": 1.36e-133, "gene_start": 1, "gene_end": 205, "reference_start": 1, "reference_end": 205, "reference_sequence": "MYVHLDELENLYANTSGELSIAESDLEAKRPAKKSFSFPDFKLPKMKLLSELKILIILFIIVFSGFFFFTNARLVLLTINDTFATEPKAAPEDLKITSVPTSQDHIKQKAQKLNELEDQFTSLQASFRTEKELAPTMKEFLAKKQETHSIEFNTLPPTNRLIIPDLNVNTPLIDVPIKGEEDFSEGQFDEELMQGAVKYPTTPAPGTEGNALIFAHSSTEWWKHNEYGFIFRNLPKLQAGQKIQIVWNGQLSTYEMLERKIIYPKDVAEYYHEFQDQKGSFITLMGCYPIGSNAQRIMVVAKKINNEDANA" }, "paralog": false, "identity": 0.9901960784313726, "score": 604.0, "evalue": 2.83e-222, "description": "Internal stop codon at 129717. Nonsense mutation around 129717.", "psc": { "uniref90_id": "UniRef90_A0A328ECY1", "product": "Sortase", "uniref50_id": "UniRef50_A0A328ECY1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328ECY1", "UniRef:UniRef50_A0A328ECY1" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328ECY1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328ECY1" ], "product": "Sortase" } }, "id": "NKOCPLAKPK_1859", "locus": "GCA003260355_01850" }, { "type": "cds", "sequence": "contig_2", "start": 130459, "stop": 130812, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAACGGTTATTCCTTTTTGCTCAGCTGGTATATCACAGCACCAATGCTTATTATAAGCAGCAAGAACGCTCGTTTTGCCCAAGTCGCAGAAATGCTGATTGAGAAACTTCGGCTATTTGCGAGATCGGCCATTCGGAAGATAAAAGTTAAGAGCCGCTGTAGAGGGAGGGCAAGCCATAGCCAATGCGTCCAAATGACTATTATTACTGTGATAAAACCACCGATCGTGATAATTGGCACCAAAGGAACGATCAAAAGGTTGATGAGCACGCCGGTGAGGTTCGTTTCTCCAATGAAGAAAAGCAGAATGGGGAGGGTTCCGAATGTTGCTCCGAGGGTTGGGAGTCCGTAG", "aa": "MNGYSFLLSWYITAPMLIISSKNARFAQVAEMLIEKLRLFARSAIRKIKVKSRCRGRASHSQCVQMTIITVIKPPIVIIGTKGTIKRLMSTPVRFVSPMKKSRMGRVPNVAPRVGSP", "aa_hexdigest": "f70ec712c1938e9b6466dcb1efb58e25", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13115.804299999982, "isoelectric_point": 11.877542304992676 }, "id": "NKOCPLAKPK_1860", "locus": "GCA003260355_01851" }, { "type": "cds", "sequence": "contig_2", "start": 130842, "stop": 131708, "strand": "-", "frame": 3, "gene": null, "product": "ComEC/Rec2-related protein domain-containing protein", "db_xrefs": [ "KEGG:K02238", "SO:0001217", "UniRef:UniRef50_A0A328EGC9", "UniRef:UniRef90_A0A328EGC9" ], "nt": "ATGGAAGAAGTCCTCATCCTTGAACAAACCAAACCGAACACTTACATCGTCCAGACCAACAACGGAAAAACATATTTCCTCAAGACCGAGATGCCGCACCAGATAGGAAAAAGCCTGCACCTAGGAGCCACCCTAAAAACCATCGATCCATCAAAACTCTACACAGAGCTGCTTTTCAGTGGAAGCGGAGAAAACTTCCGAGACTACCAATTCAACTATGATAAACGGCTGCTAATGAAAGACATCTCAGGAACCCTGTATGAAAAATTCTCCCTTCCAGCTCAACAAAATAAAATCCAAGCATCTTGGCTCCATCATCAACTAAGCACCACCAGAACCCGACTCAAAGAACGCATCCAAAAAGTATTTCCTGACAAAGAAGCCGCCTTACTCTTGGGAATGCTGATCGGAGACAAGTCCCAGCTAGAAACGGGAGCCTATCAAAACTTTGTAAACAGTGGGATCGTGCATATCATTGCCGTCAGCGGAGGAAATCTCGTGATGATTGTCGTATTTCTCAGTGCTGTGCTCTTTTGGGTTCCTTTCTATATCAGAAATGGATTGATTATTCTCGGAGTGGTTGCCTTCGCACTGCTCTGTGGTGGGGATTCCAGTGTGCTAAGAGCTTTAATTATGGCAGTGCTTTCGCTTCTGGCAGTATTCCGAGGGAGAGAAATCAAGATTTGGAGGCTTTTGAAATATGCCTTCGTCCTCATGCTCTGCTATAATCCCTTCTTTCTGATTTACGATCTAGGATTCCTCCTGAGCTTTGGAGCGGTAATCGGGATTGTCCTCATCAACGAATGGCGGACAAAATTCCAAAAAATCCCCCCTTCCAAACCAAAGGTAAAAGAGAAAAAAATCTGA", "aa": "MEEVLILEQTKPNTYIVQTNNGKTYFLKTEMPHQIGKSLHLGATLKTIDPSKLYTELLFSGSGENFRDYQFNYDKRLLMKDISGTLYEKFSLPAQQNKIQASWLHHQLSTTRTRLKERIQKVFPDKEAALLLGMLIGDKSQLETGAYQNFVNSGIVHIIAVSGGNLVMIVVFLSAVLFWVPFYIRNGLIILGVVAFALLCGGDSSVLRALIMAVLSLLAVFRGREIKIWRLLKYAFVLMLCYNPFFLIYDLGFLLSFGAVIGIVLINEWRTKFQKIPPSKPKVKEKKI", "aa_hexdigest": "7e4efd977b277e1406e45ba151be506a", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 130539, "stop": 132008, "observations": { "mutation": [ 130844 ], "stop": [ 130844 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EGC9", "query_cov": 1.0, "subject_cov": 0.5549132947976878, "identity": 0.965, "score": 538.0, "evalue": 4.48e-189, "gene_start": 1, "gene_end": 288, "reference_start": 107, "reference_end": 394, "reference_sequence": "MFLASFALFTRAFTSFLRFKRGILFFIGIFFLSLLILLLITKTQKKEKSFFFAKLKRIILTQTSAFLITSLVISLTLRKQDQNLISNFFSFAPLRIETPQSAFIPYIEEVIVLEQTKPNTYIVQTNNEKTYFLKTETPHHIGKSLHLGATLKTIDPSKLYTELLFSGGGENFRDYQFNYDKRLLMKGISGTLYEKFSLPAQQNKIQASWLHHQLSTTRTRLKERIQKVFPDKEAALLLGMLIGDKSQLETGAYQNFVNSGIVHIIAVSGGNLVMIVVFLSAVLFWVPFYIRNGLIILGVIAFALLCGGDSSVLRALIMAVLSLLAVFRGREIKIWRLLKYAFVLMLCYNPFFLIYDLGFLLSFGAVIGIVLINEWRTKFQKFPPSKPKVKEKKIGLPCLTGFLKNYGLPTLGATLGTLPILLFFIGETNLTGVLINLLIVPLVPIITIGGFITVIIVIWTHWLWLALPLQRLLTFIFRMADLANTRSFSMSISATWAKRAFLLLIISIGAVIYQLSKKE" }, "paralog": false, "identity": 0.8916155419222904, "score": 863.0, "evalue": 5.66e-320, "description": "Internal stop codon at 130844. Nonsense mutation around 130844.", "psc": { "uniref90_id": "UniRef90_A0A328EGC9", "product": "ComEC/Rec2-related protein domain-containing protein", "uniref50_id": "UniRef50_A0A328EGC9", "kegg_orthology_id": "K02238", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EGC9", "UniRef:UniRef50_A0A328EGC9", "KEGG:K02238" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EGC9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EGC9" ], "product": "ComEC/Rec2-related protein domain-containing protein" } }, "id": "NKOCPLAKPK_1861", "locus": "GCA003260355_01852" }, { "type": "cds", "sequence": "contig_2", "start": 131800, "stop": 132069, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAATGTGATTACTATACCAGCGAGTAATAAGGCTAGGCTTTCGGTCTGGAGGAGGCGTTTGCAATTTGCGAAGAAAAAGGGTTTCCTTTGTTTGCTTGCTCGCGTGATGAGGAAAAGAATCAAGACGCTCAGGAGGCAAGCTCCAGCAAAGAAAATTAGTCCTCGCTTGAGGCGGAGGAAGCTCGTAAAAGCTCGAGTAAACAATGCAAAGCTTGCAAGGAACATCGGCTTTAGGTTTAGGGGGGTAAAGTATGTTGATGAAGTGTAG", "aa": "MNVITIPASNKARLSVWRRRLQFAKKKGFLCLLARVMRKRIKTLRRQAPAKKISPRLRRRKLVKARVNNAKLARNIGFRFRGVKYVDEV", "aa_hexdigest": "d6b3267d0628131948515f0b96f75500", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10528.72049999999, "isoelectric_point": 11.999967765808105 }, "id": "NKOCPLAKPK_1862", "locus": "GCA003260355_01853" }, { "type": "cds", "sequence": "contig_2", "start": 132214, "stop": 132801, "strand": "-", "frame": 2, "gene": null, "product": "TNFR-Cys domain-containing protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBF6BAC", "UniRef:UniRef100_A0A328EC99", "UniRef:UniRef50_A0A328EC99", "UniRef:UniRef90_A0A328EC99" ], "nt": "ATGAAAGTATTCAAAACTATTTTACGAACAACCCTTTTTTGGCTTTTGGCACTCGTGGGACTCCGAATCTACACGAGCTACGAGAGTGAAAATAGCAATCTCGTTCACTACCGAGCAAGTGCTTTACCAGAATCCGTAATACAGCACCTCCACAATCAATATAATTGTAGTTGTCCGAATTGCGATACCGCTACAGGCAACAACGAAACCGCTACTACAGATGAAACTCCTACTACAGACCTAGCAGCCAGTATCCCAGCGACCTGCAAAAGCTTCTACGATGGATGCAATACGTGCAATAGGCTAGCAGATGGGGGAACGGCTTGCACCAGAATGGCATGTGAAAGTTATGGAGAGCCTAAATGCTTAGACGAAGAAACTACGACAGGGACAACGACTTCCCTCCTCCCTACAGCCTCTCAACCAACGACAACTGAAGGGCTAAGCTCACTTGAAAAGAGAATCACAGAGCTTGAAAAGAATCACTGGGCACTCGTGCAGGAGTTGCAAACGATTTTCTCTACTCCAGAGGGACAGAAACTCCTTCCTGCTCCTACTCCAGTAGCAAACCCAGCCGCTCAAAACTAA", "aa": "MKVFKTILRTTLFWLLALVGLRIYTSYESENSNLVHYRASALPESVIQHLHNQYNCSCPNCDTATGNNETATTDETPTTDLAASIPATCKSFYDGCNTCNRLADGGTACTRMACESYGEPKCLDEETTTGTTTSLLPTASQPTTTEGLSSLEKRITELEKNHWALVQELQTIFSTPEGQKLLPAPTPVANPAAQN", "aa_hexdigest": "ed6fcf6b696f9ca45066ceccbe3d8238", "start_type": "ATG", "rbs_motif": "TTATT", "ups": { "uniparc_id": "UPI000DBF6BAC", "uniref100_id": "UniRef100_A0A328EC99", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBF6BAC", "UniRef:UniRef100_A0A328EC99" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EC99", "uniref90_id": "UniRef90_A0A328EC99" }, "psc": { "uniref90_id": "UniRef90_A0A328EC99", "product": "TNFR-Cys domain-containing protein", "uniref50_id": "UniRef50_A0A328EC99" }, "pscc": { "uniref50_id": "UniRef50_A0A328EC99", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EC99" ], "product": "TNFR-Cys domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_1863", "locus": "GCA003260355_01854" }, { "type": "cds", "sequence": "contig_2", "start": 132826, "stop": 133329, "strand": "-", "frame": 2, "gene": null, "product": "Pseudouridine synthase RsuA/RluA-like domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_K1X4I8", "UniRef:UniRef90_A0A328EC92" ], "nt": "GTGCTCATCGCCGCAAAAACCTACGAGTCCCTGCAATACATCAACCAAATCATCAGAGAAAGGGCAATCAGCAAAGAATACCTGACTCGAGTCGCAGGAAAATTTCCCAAGCATCTCATCATTGATAAAGCAATAGAAAAAACCTACAACGCCAAATTCGCCAGAGCTCAGATGCAGCTCAACGAAAGTGATGGACTAGATTCAAAAACTGAATGCCGATGTGAAAAAACGCTCCAGCATAACGAGCTCGGTCAGATTTCCCTCGTTAGGGTAAAGCTCTATAGCGGCAGAATGCACCAAATCAGAATCCACCTTGCAAGCGAAAACTTCCCTGTTTTGGGGGATCTCATCTATGGGAAACCCGCAATCAATAGAATTGTATACAAAAGCTTGCATATTCAGAGACAATTATTACACTGTTGGAAGTATCAGTTCAAAAACCTTAATGGAAAGCTGATTGCCTTCGAGGCTCCACTTCCTCAGGAGTTCAAACTGTTTACTTAA", "aa": "MLIAAKTYESLQYINQIIRERAISKEYLTRVAGKFPKHLIIDKAIEKTYNAKFARAQMQLNESDGLDSKTECRCEKTLQHNELGQISLVRVKLYSGRMHQIRIHLASENFPVLGDLIYGKPAINRIVYKSLHIQRQLLHCWKYQFKNLNGKLIAFEAPLPQEFKLFT", "aa_hexdigest": "da135053eef509600026ce35ae56fc6b", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 132826, "stop": 133629, "observations": { "mutation": [ 133359 ], "stop": [ 133359 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EC92", "query_cov": 1.0, "subject_cov": 0.5075987841945289, "identity": 0.9940000000000001, "score": 335.0, "evalue": 3.2e-114, "gene_start": 1, "gene_end": 167, "reference_start": 163, "reference_end": 329, "reference_sequence": "MELTITPDQANQRCDRFLRKFCKPYPQVRLSDIYSWIRKGEIKVNGKKTKEETRLHEGDLLELPESLLGDKDKQLMLTQKEKKFQKLDPKLVKSWILYEDQQRLAFNKPAGIVLHPSNKHRNDLCMNDYLERYCSRSEGQKEEKNQTFKPSFGYRLDKDTSGVLIAAKTYESLQYINQIIRERAISKEYLTRVAGKFPKHLIIDKAIEKTYNAKFARAQMQLNESDGLDSKTECRCEKTLQHNELGQISLVRVKLYSGRMHQIRIHLASENFPVLGDLIYGKPAINRIVYKSLHIQRQLLHCWKYQFKNLNGKLIAFEAPLPQEFKLFT" }, "paralog": false, "identity": 0.9850187265917603, "score": 533.0, "evalue": 1.3e-194, "description": "Internal stop codon at 133359. Nonsense mutation around 133359.", "psc": { "uniref90_id": "UniRef90_A0A328EC92", "product": "Pseudouridine synthase RsuA/RluA-like domain-containing protein", "uniref50_id": "UniRef50_K1X4I8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EC92", "UniRef:UniRef50_K1X4I8" ] }, "pscc": { "uniref50_id": "UniRef50_K1X4I8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_K1X4I8" ], "product": "Pseudouridine synthase RsuA/RluA-like domain-containing protein" } }, "id": "NKOCPLAKPK_1864", "locus": "GCA003260355_01855" }, { "type": "cds", "sequence": "contig_2", "start": 133357, "stop": 133860, "strand": "-", "frame": 2, "gene": null, "product": "Pseudouridine synthase RsuA/RluA-like domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_K1X4I8", "UniRef:UniRef90_A0A328EC92" ], "nt": "TTGCGTTTTTTCTATACTAGGAAACGCTTTATCCTCGCCGCTCCAATGGAACTAATAATTACTCCCGACCAAGCCAACCAGAGATGCGATCGCTTCCTGAGAAAATTTTGTAAACCCTATCCGCAGGTCAGACTTTCTGACATCTATTCTTGGATTCGTAAAGGAGAAATCAAAGTAAATGGAAAAAAAACAAAAGAAGAAACCAGACTCCATGAAGGCGACCTGCTTGAGCTCCCAGAATCACTCCTCGGCACCAAAGACAAACAGCTTATGCTCACGCAAAAAGAAAAAAAATTCCAAAAGCTAGACCCTAAACTCATAAAATCATGGATTCTCTACGAAGACGAGCAGCGACTCGCATTTAATAAGCCGGCTGGGATCGTCCTCCACCCAAGTAATAAACACCGAAACGATCTCTGTATGAACGATTACTTAGAACGATACTGCTCGAGAAGCGAGGGACAAAAAGAGGAAAAAAATCAGACTTTTAAACCTTCTTTTTGA", "aa": "MRFFYTRKRFILAAPMELIITPDQANQRCDRFLRKFCKPYPQVRLSDIYSWIRKGEIKVNGKKTKEETRLHEGDLLELPESLLGTKDKQLMLTQKEKKFQKLDPKLIKSWILYEDEQRLAFNKPAGIVLHPSNKHRNDLCMNDYLERYCSRSEGQKEEKNQTFKPSF", "aa_hexdigest": "8cf9785e1e452bc86c46c004f55cdfb9", "start_type": "TTG", "rbs_motif": "TTTA", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 133054, "stop": 133815, "observations": { "mutation": [ 133359 ], "stop": [ 133359 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EC92", "query_cov": 0.9101796407185628, "subject_cov": 0.46200607902735563, "identity": 0.9740000000000001, "score": 303.0, "evalue": 2.64e-101, "gene_start": 16, "gene_end": 167, "reference_start": 1, "reference_end": 152, "reference_sequence": "MELTITPDQANQRCDRFLRKFCKPYPQVRLSDIYSWIRKGEIKVNGKKTKEETRLHEGDLLELPESLLGDKDKQLMLTQKEKKFQKLDPKLVKSWILYEDQQRLAFNKPAGIVLHPSNKHRNDLCMNDYLERYCSRSEGQKEEKNQTFKPSFGYRLDKDTSGVLIAAKTYESLQYINQIIRERAISKEYLTRVAGKFPKHLIIDKAIEKTYNAKFARAQMQLNESDGLDSKTECRCEKTLQHNELGQISLVRVKLYSGRMHQIRIHLASENFPVLGDLIYGKPAINRIVYKSLHIQRQLLHCWKYQFKNLNGKLIAFEAPLPQEFKLFT" }, "paralog": false, "identity": 0.9802371541501976, "score": 503.0, "evalue": 9.66e-183, "description": "Internal stop codon at 133359. Nonsense mutation around 133359.", "psc": { "uniref90_id": "UniRef90_A0A328EC92", "product": "Pseudouridine synthase RsuA/RluA-like domain-containing protein", "uniref50_id": "UniRef50_K1X4I8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EC92", "UniRef:UniRef50_K1X4I8" ] }, "pscc": { "uniref50_id": "UniRef50_K1X4I8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_K1X4I8" ], "product": "Pseudouridine synthase RsuA/RluA-like domain-containing protein" } }, "id": "NKOCPLAKPK_1865", "locus": "GCA003260355_01856" }, { "type": "cds", "sequence": "contig_2", "start": 133911, "stop": 134807, "strand": "-", "frame": 3, "gene": null, "product": "RNA polymerase sigma-70 region 4 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A288DJV0", "UniRef:UniRef90_A0A328EEF6" ], "nt": "ATGAGATTCAGAAGACTCATTGTAGGAAATGAAGCATACATCAACGTCCTAGAGGAAACCAAAAAAATCCTTGAGATCCATGGAGGACTTCTTGACGAGGACACCCTCCTTGCAAAGATAATGAACAAAAATGTTCAAAAGTTTTCAAAGCCAGAATTAAAATTAATTCTGATTTCCGACTTTGATATCACCTATCTCAAAAGAAATAAATATCTCTCTAAAGCATTCTATATCGAGCCTCTTTACGAGGACCTTCTCACAAAAATGGTAATTTTTAGCCAAAATTACTTCACAAAGAGAAAGGAAAGCCAAGACCTCTACGAATTTATCGGGACCCTAAAGGAGGAGTTCCTAGAGGAATTCAGAGACATCAGCTTCCTAAAAAACGATCTCTTCTATATCAACTTTTTCCGCATTGTAAAAGGTATGGCAATTTTGGATGGAAAGATTGGACTGGTAGAATTTCCAGACATCTATCCAAAAACTCTGAAGCTCAAGATCCTTTATACGATGAGAAGACTCAATAAACCAATCCATTACCAAGAGCTCCCAGCAAAAATTATGGAGCGATTCCCAGAATGCTCTATCAAAGCAAATTCCGTACACAACGACCTCGTCAAACACAACGATATCTTTGTAAACTTAGGACTAGGACTTTACGGACTCAAAGAGCGAGGATATGAAGGAGGAAATGTAAAAGATATTCTCATAAGAGTTTTCAAAAGATTTCAAAAGCCGATGACCGTAAAGGAATTAGCGAGAGAAGTGCTAAGGGAAAAAATGGTGAGTCAAAACACCATCCTCCTTAATCTCCAAAAATACAATACTACCTTTGAGAAAATCGAAAAAGGACTCTATGCTTATAGAGAGAATCCACCGCACAAAACCAAGAAATAA", "aa": "MRFRRLIVGNEAYINVLEETKKILEIHGGLLDEDTLLAKIMNKNVQKFSKPELKLILISDFDITYLKRNKYLSKAFYIEPLYEDLLTKMVIFSQNYFTKRKESQDLYEFIGTLKEEFLEEFRDISFLKNDLFYINFFRIVKGMAILDGKIGLVEFPDIYPKTLKLKILYTMRRLNKPIHYQELPAKIMERFPECSIKANSVHNDLVKHNDIFVNLGLGLYGLKERGYEGGNVKDILIRVFKRFQKPMTVKELAREVLREKMVSQNTILLNLQKYNTTFEKIEKGLYAYRENPPHKTKK", "aa_hexdigest": "8f534bec3dbaf2e81aaa018e023f9247", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A328EEF6", "query_cov": 1.0, "subject_cov": 0.8054054054054054, "identity": 0.997, "score": 583.0, "evalue": 3.67e-209, "valid": true, "product": "RNA polymerase sigma-70 region 4 domain-containing protein", "uniref50_id": "UniRef50_A0A288DJV0" }, "pscc": { "uniref50_id": "UniRef50_A0A288DJV0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A288DJV0" ], "product": "RNA polymerase sigma-70 region 4 domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_1866", "locus": "GCA003260355_01857" }, { "type": "cds", "sequence": "contig_2", "start": 134901, "stop": 135023, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGATTTATTGCAAAAATGGATGATAAAAAAATTAAAGTAGATATAGAAACTACTCAATTAAAAGATGTTCTCATTTACTGCAAACCAAAGGAAAGGCTGGTTTTAATGAGAAAGTTTTGA", "aa": "MGFIAKMDDKKIKVDIETTQLKDVLIYCKPKERLVLMRKF", "aa_hexdigest": "f77589c728f88defe5249d44532407d9", "start_type": "ATG", "rbs_motif": "TTATT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4786.829599999999, "isoelectric_point": 9.517548179626463 }, "id": "NKOCPLAKPK_1867", "locus": "GCA003260355_01858" }, { "type": "cds", "sequence": "contig_2", "start": 135184, "stop": 135777, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MZA9", "UniRef:UniRef90_A0A660MZA9" ], "nt": "ATGAATCTTAGCGGAGAGGCATCCTCTCTCTCTGGAGAGCAAAGTTTATCTGGAGACAATATCAACCTAATCATCAACGAAACAGGTGCAGAAGCCAATGGCCTCACTTCAGGAGAATCATCTTCTTTACCTAGCGGAACAAATCTCTCTACAGGACTGAGCGTCCAAAGAATTGAAGAACCTACAAATACGCAAACTCCCGCCAGCACACAAACCCAGACTTCAAACCAGCCTTCAAACAAGACAAGCAGCCCAAACCAAGAAGTGACGATGATTGATGCCATTAAACATCTTATCAAAACAAATAATATTCCTCTTTCTACCAAGAAAAATACAAAATTCACGCACATAGCAACTTCCGACAGTAATTATCCTTATATGAAAACAGCGCTTGAAAAAAGAATGATCGGCTCTAATACCAATCCAAACATGACCGTCAGCTGTGATATTTATATGGTAATGAAAGGCTTGGCAGAAGAACGAATTACCGTAAAAACCGCGGATGTAAAAGGTGACTATCGAAATATGGCAGAGTCTAAAAACGCCCTCAATGGTTGCACAAAAGGAGCAAAATTAACCATAGCTAACTTATAG", "aa": "MNLSGEASSLSGEQSLSGDNINLIINETGAEANGLTSGESSSLPSGTNLSTGLSVQRIEEPTNTQTPASTQTQTSNQPSNKTSSPNQEVTMIDAIKHLIKTNNIPLSTKKNTKFTHIATSDSNYPYMKTALEKRMIGSNTNPNMTVSCDIYMVMKGLAEERITVKTADVKGDYRNMAESKNALNGCTKGAKLTIANL", "aa_hexdigest": "0ef241d335800d5fe10fbf06d61e1485", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 135184, "stop": 136077, "observations": { "mutation": [ 135828, 135897, 135933, 136068 ], "stop": [ 135828, 135897, 135933, 136068 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A660MZA9", "query_cov": 1.0101522842639594, "subject_cov": 0.44320712694877507, "identity": 0.935, "score": 320.0, "evalue": 5.89e-106, "gene_start": 1, "gene_end": 197, "reference_start": 251, "reference_end": 449, "reference_sequence": "MTNMTKKGPIIISIIYIFVIQAIIIGLLRNEPRRYILGGINFIATILLALLGKQSKQRSEQKGEAPLIREKHPTPKETAEKDFFTKEKKEESSPTLSESNIEYIKTRINRNRKNQTTNGGGLYRLLIFLLALIGFGGILYMFREIFDFIAIFIGAIGMMIAIMVIFRATQLRERPIFSSLYFLIFFVLGCGGLAASLFGGKYEKIEDIKNGISTFIEGVKGKEITNTETGNNEGEINSDYLFEGTGSVIEMNLSGDVSSLSGEQSLSGDNINLIINETGAEANGLTSGESSSLPSGTNLSTGLSVERIEEPTNTQTPANTQTPANTQTQTSNKTSSPNQEVTMIDAIKHLIKTNNIPLSTKKNTKFTHIATSDSNYPYMKTALEKRMIGSNTNPNMTVSCDIYMVMKGLAEERITVKTADVKGDYRNMAESKNALNGCTKGAKLTVANL" }, "paralog": false, "identity": 0.9230769230769231, "score": 506.0, "evalue": 1.33e-181, "description": "Internal stop codon at 135828, 135897, 135933, 136068. Nonsense mutation around 135828, 135897, 135933, 136068.", "psc": { "uniref90_id": "UniRef90_A0A660MZA9", "uniref50_id": "UniRef50_A0A660MZA9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A660MZA9", "UniRef:UniRef50_A0A660MZA9" ] }, "pscc": { "uniref50_id": "UniRef50_A0A660MZA9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MZA9" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 21022.269499999995, "isoelectric_point": 6.736011695861817 }, "id": "NKOCPLAKPK_1868", "locus": "GCA003260355_01859" }, { "type": "cds", "sequence": "contig_2", "start": 136881, "stop": 137333, "strand": "+", "frame": 3, "gene": null, "product": "AmmeMemoRadiSam system protein B", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MZZ7", "UniRef:UniRef90_A0A660MZZ7" ], "nt": "ATGTTTTCTCATATCTCTCGTCAGCGTCAGTATTTTCTGGCTGTTATTCCTCATTTTATGATTGACCCGGTAAAAGTTCAGGATTTTTATTCTTTTTTGAAGGGGCGTTTTTTTTCTTCTCATGAAATGCCAGATCAAATTGTTCTTCTCTCTCCTAATCATTTTTTTCTGAAACAAAAACGTGTAGAAGGACTTTGTACTTCTGGGAGAATTAGGTTTAAAGATCAGACTATTGTTGCTACTCCTTTTGTAGATGATCGTATTGCTTGTGATGGAGGGGTTTTTTATGAGAAAGGAGGGCAAACTTATACGAAAGATCATGGTTTGGGAGAGCATTTTCGCTGGATTAATCAGTTTTTCCCTACTGTTAGAACTGTTTATCTTTTTGCTCTTCCTACACATCATATGGAGCACGCTTCTTGGCTGGCTGAAAATATCCAAAAACTGCCCTGA", "aa": "MFSHISRQRQYFLAVIPHFMIDPVKVQDFYSFLKGRFFSSHEMPDQIVLLSPNHFFLKQKRVEGLCTSGRIRFKDQTIVATPFVDDRIACDGGVFYEKGGQTYTKDHGLGEHFRWINQFFPTVRTVYLFALPTHHMEHASWLAENIQKLP", "aa_hexdigest": "f36f0fc5c5f41b52f1690b8b4a570a72", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 136803, "stop": 137615, "observations": { "mutation": [ 136830, 137331 ], "stop": [ 136830, 137331 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A660MZZ7", "query_cov": 0.9933333333333333, "subject_cov": 0.5518518518518518, "identity": 0.973, "score": 306.0, "evalue": 5.59e-104, "gene_start": 2, "gene_end": 150, "reference_start": 28, "reference_end": 176, "reference_sequence": "MLMSTRKKIGFWCLGVLLVSAVSIFFTFSRISRQRQYFLAVIPHFMIDPVKVQDFYSFLKGRFFSSHEMPDQIVLLSPNHFFLKQKRVEGLCTLGRIRFKDQTIVATPFVDDRIACDGGVFYEKGGQIYTKDHGLGEHFRWINQFFPTVRTVYLLALPTHHMEHASWLAENIQKLPGKTLVIASVDFSHYLSEVIAEQHDQVSFSVLQQSGAFDQLRRLDVDCPACLGVVYRLASREKIRVHQRWRDSSSTIVGKDLMDQNTSRQFLWWE" }, "paralog": false, "identity": 0.9592592592592593, "score": 523.0, "evalue": 7.01e-192, "description": "Internal stop codon at 136830, 137331. Nonsense mutation around 136830, 137331.", "psc": { "uniref90_id": "UniRef90_A0A660MZZ7", "product": "AmmeMemoRadiSam system protein B", "uniref50_id": "UniRef50_A0A660MZZ7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A660MZZ7", "UniRef:UniRef50_A0A660MZZ7" ] }, "pscc": { "uniref50_id": "UniRef50_A0A660MZZ7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MZZ7" ], "product": "AmmeMemoRadiSam system protein B" } }, "id": "NKOCPLAKPK_1869", "locus": "GCA003260355_01860" }, { "type": "cds", "sequence": "contig_2", "start": 137355, "stop": 137615, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGATTTTTCTCATTATTTGTCAGAGGTTATTGCCGAGCAACATGATCAGGTTTCTTTTTCTGTTTTGCAGCAAAGTGAGGCTTTTGATCAACTAAGGAGATTGGATGTTGATTGCCCTGCCTGTTTGGGGGTTGTTTATCGTCTTGCTTCAAGGGAGAAAATTAGGGTCCATCAGCGATGGAGAGATTCATCAAGCACTATCGTTGGAAAGGATCTGATGGATCAAAACACTTCTAGGCAATTCCTTTGGCGGGAGTAG", "aa": "MDFSHYLSEVIAEQHDQVSFSVLQQSEAFDQLRRLDVDCPACLGVVYRLASREKIRVHQRWRDSSSTIVGKDLMDQNTSRQFLWRE", "aa_hexdigest": "e4615c7e64a024dd57844a575b4479f3", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10159.285499999994, "isoelectric_point": 5.8666593551635735 }, "id": "NKOCPLAKPK_1870", "locus": "GCA003260355_01861" }, { "type": "cds", "sequence": "contig_2", "start": 138647, "stop": 138841, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBF3F90", "UniRef:UniRef100_A0A328ECZ8", "UniRef:UniRef50_A0A328ECZ8", "UniRef:UniRef90_A0A328ECZ8" ], "nt": "ATGAAAAGAAAATACTCAGATTTTTTCAGTACTATCCTCTTGTTGACTGTGATGATACTCTTGTTTGTGTTCGTCTTGTCGCTTCTTTCTGGAGTTTGGTATTGTATCGGGATTAGTTTTGCTGCTTATGTCCTTGCACTTGGGGGATCTCATTATTATGAGTTCTCCTATGATGAACAACTTCCCCCTGAATAG", "aa": "MKRKYSDFFSTILLLTVMILLFVFVLSLLSGVWYCIGISFAAYVLALGGSHYYEFSYDEQLPPE", "aa_hexdigest": "939544f4ab8aef2c0950175570683331", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000DBF3F90", "uniref100_id": "UniRef100_A0A328ECZ8", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBF3F90", "UniRef:UniRef100_A0A328ECZ8" ] }, "ips": { "uniref100_id": "UniRef100_A0A328ECZ8", "uniref90_id": "UniRef90_A0A328ECZ8" }, "psc": { "uniref90_id": "UniRef90_A0A328ECZ8", "uniref50_id": "UniRef50_A0A328ECZ8" }, "pscc": { "uniref50_id": "UniRef50_A0A328ECZ8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328ECZ8" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7367.594899999999, "isoelectric_point": 5.050678825378417 }, "id": "NKOCPLAKPK_1871", "locus": "GCA003260355_01862" }, { "type": "cds", "sequence": "contig_2", "start": 139089, "stop": 139289, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCAACTTGTGGTTGGTGGAGATATTATGCTTTCCAGGAATATTGGATGGCTCGCTAAACAAGAGGGGTATGATCGTACCTTTTCTGGTCAGAATTTTAATCCTCTTTCTCAATTTTCGTGCTATGCATCAGGAGACTGTCTGCTCTACTTTAATCTTGAAAGTATGTTTTCTGCTAAAGATAATGATCAGCCAAAGTGA", "aa": "MQLVVGGDIMLSRNIGWLAKQEGYDRTFSGQNFNPLSQFSCYASGDCLLYFNLESMFSAKDNDQPK", "aa_hexdigest": "f331b2c4c722778fa2a16255588c3d07", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7448.297199999998, "isoelectric_point": 4.6018781661987305 }, "id": "NKOCPLAKPK_1872", "locus": "GCA003260355_01863" }, { "type": "cds", "sequence": "contig_2", "start": 139591, "stop": 139767, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAATGCCCTCCAAGAAGAATATCAGCTCCAGCATCGACAAGCTGATGTGCCAAGAGAATCTGCCATTTCGCTGGTTGGAAGCGATATTCTGCTCACCAATGAAGCCCTAATACCTTCACATCACAAAGATCTTGCTCCATCGCATGAAGAGTCCCGGTCATCAAAGGCAAACTAA", "aa": "MNALQEEYQLQHRQADVPRESAISLVGSDILLTNEALIPSHHKDLAPSHEESRSSKAN", "aa_hexdigest": "eaee121e949bc347b2976133f35a454e", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6463.038799999998, "isoelectric_point": 5.3651121139526365 }, "id": "NKOCPLAKPK_1873", "locus": "GCA003260355_01864" }, { "type": "cds", "sequence": "contig_2", "start": 139851, "stop": 140078, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGACCTCTGTTGGAGAATTTGATTTTAACTATGATCATGAACTGAAGAAAAATAGAGTGCCTACGTATATTCCCCTATTGGCTTCATTACAAATTGAAAAAAATGGCACAGGTATTCAGATTTCTTTTCTTCTATTTAAAATGGCTCGTTTATATAAAGGAATTTTCTCTCCTCTTGATGATGAAACCTTTTCTGGTATGATGGAGCATTTGGTGTTAAAATAG", "aa": "MKTSVGEFDFNYDHELKKNRVPTYIPLLASLQIEKNGTGIQISFLLFKMARLYKGIFSPLDDETFSGMMEHLVLK", "aa_hexdigest": "569526fd86e58f5adde3cab34b8bd52d", "start_type": "ATG", "rbs_motif": "TTTTT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8657.020599999998, "isoelectric_point": 6.715379142761231 }, "id": "NKOCPLAKPK_1874", "locus": "GCA003260355_01865" }, { "type": "cds", "sequence": "contig_2", "start": 140201, "stop": 140554, "strand": "+", "frame": 2, "gene": "rmuC", "product": "DNA recombination protein RmuC", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7W4EC79", "UniRef:UniRef90_A0A660N001" ], "nt": "GTGATCGCGTATTTGATCTTTTATTTTCAGTATGAGAGAAGGGAAATCGTTTCAGAACTTAGGAAAAACCTAAAGTCTGCGAATGAACAGCTCAGTTATCTTCAAGAAGAGATGGAGGAATTGACCGCGCAAAACCAGCTTTTTAGAGAAAAAACTACAGAGCTTCTTGAGAAAAATGATGAACTTTCAGATGTTGTGGCAGAATTGGGAAAGTATTATGTTCATATGAAAAAAGCTTCCGAAAAGACGGCAGAACTTTCTAAATTTTTGCATGAGCCAGATGAGGAGATGGCACTAAAACTTGATACTTTGTCAGAAAAAACTGAACGTCAGTTGAAGAACCGTTTTTTTTAA", "aa": "MIAYLIFYFQYERREIVSELRKNLKSANEQLSYLQEEMEELTAQNQLFREKTTELLEKNDELSDVVAELGKYYVHMKKASEKTAELSKFLHEPDEEMALKLDTLSEKTERQLKNRFF", "aa_hexdigest": "3aaa67f4a46d5129349da6df59d571b6", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A660N001", "query_cov": 1.0, "subject_cov": 0.9, "identity": 0.991, "score": 214.0, "evalue": 4.72e-70, "valid": true, "product": "DNA recombination protein RmuC", "uniref50_id": "UniRef50_A0A7W4EC79" }, "pscc": { "uniref50_id": "UniRef50_A0A7W4EC79", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7W4EC79" ], "product": "DUF1043 family protein" }, "genes": [ "rmuC" ], "id": "NKOCPLAKPK_1875", "locus": "GCA003260355_01866" }, { "type": "cds", "sequence": "contig_2", "start": 140637, "stop": 141770, "strand": "+", "frame": 3, "gene": null, "product": "TGS domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EGE1", "UniRef:UniRef90_A0A328EGE1" ], "nt": "ATGGATATCAAGGAGATAGATAGTTTTTTTGACTATCAGATGACCTTTGATGAGGATCCGCTTTTTTTGCTTGATGAAATTATTGCTCAGGCTTCGGCTTATCTTCCAGCGGAACAGATTGAAGGTATTAAACATGCCTACACTTTTACTAAAGCTGCTCATGCTGGAGTTAAGAGGCTTTCTGGAGAGCCGTATATTGTTCATCCTCTCAAGGCAACGCTTTTTTTGATGGAGCTTAAGCCCGACTTGGCATCTATTCAAGCTTGTATTATGCATGATGTGATTGAGGATACGCCGATAACTCGTGAAGAAGTTGCTGCTGAGTTTACCGAAGAAGTAGCGGAAATTTGTGAGGGCTTGGTTAAGGTTTCAAAAGTGAGGTATCAAGGAGAAGATCGTCATTTGGAAACGATAAAAAAGACCTTTCTTGCGATGGCGAAAGATTTGAGGGTGATTTTTGTGAAATTGGCTGATCGTATTCACAATATTCAGACTTTGCAGTTTCATCCAGAAGAGAGGAAAAGGCTGAAAATTGCAGAAGAAACCCTAAAAATTTATGTTCCAATTGCTAAAAGGCTCGGGCTTTATCATTTTCAACTTCTTCTCGAAAATGGTTCTTTTGCTACAATGAAACCGGAGGAGTTTTCCAGAATTTTGAGCTATTTGAAAAAATATTTTCGTGAGGGAGAAAAATATACTGAAAAGGGAGTGAAAATGCTGACAGGGATGCTTCATAGAGAAGGGGTTCAGAATTTTGAGGTCAAGGGAAGAATTAAAAGTCCCTATAGAGTATATGAAAAGCTTTCCAAAAAGTATCATGAGACCGATATTTCTACAGTAATGGACCTGCTTGCTTTTAGGGTGATTACTCAGAGTGTCGGTGATTGTTATATGGTTTTGGGGGTTATCCATAAGTATTATATTCCACTTATCAAGAAGATTAAGGATTATATTGCGATTCCAAAATTTAATGGTTATAAGAGTATTCATACGACTGTTTTGGGGATGTTTCGTTTTCCTGTGGAAATTCAGATTAGAACTCAGGAAATGGATGATGTGGCGGAGTTTGGAGTCGCTGCTCATTTTGCTTATGCAGAAAGCAATGCTCCAACCGTGGTTTCTGAGCAGCAGTGA", "aa": "MDIKEIDSFFDYQMTFDEDPLFLLDEIIAQASAYLPAEQIEGIKHAYTFTKAAHAGVKRLSGEPYIVHPLKATLFLMELKPDLASIQACIMHDVIEDTPITREEVAAEFTEEVAEICEGLVKVSKVRYQGEDRHLETIKKTFLAMAKDLRVIFVKLADRIHNIQTLQFHPEERKRLKIAEETLKIYVPIAKRLGLYHFQLLLENGSFATMKPEEFSRILSYLKKYFREGEKYTEKGVKMLTGMLHREGVQNFEVKGRIKSPYRVYEKLSKKYHETDISTVMDLLAFRVITQSVGDCYMVLGVIHKYYIPLIKKIKDYIAIPKFNGYKSIHTTVLGMFRFPVEIQIRTQEMDDVAEFGVAAHFAYAESNAPTVVSEQQ", "aa_hexdigest": "613d90d46008dced409b7974041b732c", "start_type": "ATG", "rbs_motif": "TTTTT", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 140637, "stop": 142073, "observations": { "mutation": [ 141768, 141894, 141993, 142029 ], "stop": [ 141768, 141894, 141993, 142029 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EGE1", "query_cov": 1.0, "subject_cov": 0.5067204301075269, "identity": 1.0, "score": 737.0, "evalue": 1.09e-262, "gene_start": 1, "gene_end": 377, "reference_start": 1, "reference_end": 377, "reference_sequence": "MDIKEIDSFFDYQMTFDEDPLFLLDEIIAQASAYLPAEQIEGIKHAYTFTKAAHAGVKRLSGEPYIVHPLKATLFLMELKPDLASIQACIMHDVIEDTPITREEVAAEFTEEVAEICEGLVKVSKVRYQGEDRHLETIKKTFLAMAKDLRVIFVKLADRIHNIQTLQFHPEERKRLKIAEETLKIYVPIAKRLGLYHFQLLLENGSFATMKPEEFSRILSYLKKYFREGEKYTEKGVKMLTGMLHREGVQNFEVKGRIKSPYRVYEKLSKKYHETDISTVMDLLAFRVITQSVGDCYMVLGVIHKYYIPLIKKIKDYIAIPKFNGYKSIHTTVLGMFRFPVEIQIRTQEMDDVAEFGVAAHFAYAESNAPTVVSEQQGVWIQKLQKIVADYTDSSEKERFKSQLNIEVLDKTIFIYTPRGDIKELPAGSTVLDFAFSIHSAIGLRFKNAIVNGQIRPISYKLKTGDIVNINTFKNRYVANKHWIEFLRTPSAKNQLLKYLKTIERETRLEEAIDGLNQYLKDLGLPLFRSDRDKIQKLDDPLEVERRILMVLDKQDSYGNIVRLAYPDLLKNVVEKSIIKDTGELKDTLKQEIKNQNSTFSVIVDNDKHLNCTFCPECHPKLGDKIIVKSTRQGIKVHTMHCKALKTISFDSLLEAHWDQESPNRYHFELQIKFSSRELTIVDFLQVFSQFNVQLSEISIKHGENAEVIVDFELQVDNPSKIAFILKDLKKFSLSLEILKKVIF" }, "paralog": false, "identity": 0.9916317991631799, "score": 929.0, "evalue": 0.0, "description": "Internal stop codon at 141768, 141894, 141993, 142029. Nonsense mutation around 141768, 141894, 141993, 142029.", "psc": { "uniref90_id": "UniRef90_A0A328EGE1", "product": "TGS domain-containing protein", "uniref50_id": "UniRef50_A0A328EGE1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EGE1", "UniRef:UniRef50_A0A328EGE1" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EGE1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EGE1" ], "product": "TGS domain-containing protein" } }, "id": "NKOCPLAKPK_1876", "locus": "GCA003260355_01867" }, { "type": "cds", "sequence": "contig_2", "start": 142038, "stop": 142871, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAATATCAATACCTTTAAGAATAGGTATGTTGCGAATAAACACTGGATCGAGTTTTTGCGTACTCCATCAGCGAAAAATCAGCTTTTAAAATATTTAAAAACTATAGAAAGAGAGACGCGTTTGGAGGAAGCAATTGATGGTTTGAATCAGTATTTGAAAGATTTGGGTCTTCCTTTGTTTCGTAGTGATAGAGATAAAATTCAGAAGCTGGATAATCCTCTTGAGGTGGAGAGGAGAATTTTGATGGTATTGGATAAGCAAGATTCCTATGGAAATATTGTAAGGCTGGCTTATCCTGATTTGCTTAAAAATGTCGTGGAGAAAAGTATTATCAAAGATACTGGTGAACTCAAAGATACTTTAAAACAAGAGATAAAAAATCAGAATTCAACTTTTTCTGTTATTGTTGATAATGACAAGCATTTGAATTGTACTTTTTGTCCGGAGTGTCATCCTAAGCTTGGAGATAAAATTATCGCAAAATCTACTAGACAAGGAATTAAAGTTCACACTATGCATTGTAAAGCGTTAAAGACTATTTCGTTTGATTCTCTTTTGGAAGCTCATTGGGACCAAGAAAGCCCAAATCGTTATCACTTTGAGCTTCAGATCAAATTTTCTTCTCGTGAATTGACGATCGTAGATTTTCTTCAGGTTTTTTCTCAATTTAATGTGCAACTTTCAGAAATATCTATAAAGCACGGAGAAAATGCAGAGGTGATTGTTGATTTTGAATTGCAAGTGGATAATCCATCAAAAATCGCTTTTATTCTGAAAGACTTGAAAAAGTTTTCCCTTTCCTTAGAAATTCTGAAGAAAGTGATTTTTTAG", "aa": "MNINTFKNRYVANKHWIEFLRTPSAKNQLLKYLKTIERETRLEEAIDGLNQYLKDLGLPLFRSDRDKIQKLDNPLEVERRILMVLDKQDSYGNIVRLAYPDLLKNVVEKSIIKDTGELKDTLKQEIKNQNSTFSVIVDNDKHLNCTFCPECHPKLGDKIIAKSTRQGIKVHTMHCKALKTISFDSLLEAHWDQESPNRYHFELQIKFSSRELTIVDFLQVFSQFNVQLSEISIKHGENAEVIVDFELQVDNPSKIAFILKDLKKFSLSLEILKKVIF", "aa_hexdigest": "dc96cb7cb85d57fe433ca2b406bbab2b", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 32319.97390000003, "isoelectric_point": 8.49740047454834 }, "id": "NKOCPLAKPK_1877", "locus": "GCA003260355_01868" }, { "type": "cds", "sequence": "contig_2", "start": 143062, "stop": 143463, "strand": "+", "frame": 1, "gene": null, "product": "DZANK-type domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328ECB0", "UniRef:UniRef90_A0A328ECB0" ], "nt": "TTGGCTTCTTATTCAGATCGTGAGCTTTTGCTGTTTGGTTTTTTGGCTTTGGTTTGGCTTCTTTGTGCAATTTGGACCTTGAGAGACGCGATGGCGAGGAGTGATAGTTCATTTTACCAGTTTTTTTCTGTCCTTTTGATAGTTGTTTTGACTCCGGTTTTTGGTTTGCCGCTTTATTTGGCGTTTCGTCCTTTGGTATATAAACGAGAAAAAGGATCTTGGAGAGAGGCTTTGGAACAGACTGTTGTGGAATGTCCTCATTGTCGCGGTTTGAATTCTGTTTCTCATAAGGTTTGTGCTCGGTGTGGGGAAGCTTTGTGTGTTGAGTGTAGGGAATGTCATAGTAAATATTCTGGTCAGTTTGCATATTGTCCTGAATGTGGAGCTCCAAATATTGAATAG", "aa": "MASYSDRELLLFGFLALVWLLCAIWTLRDAMARSDSSFYQFFSVLLIVVLTPVFGLPLYLAFRPLVYKREKGSWREALEQTVVECPHCRGLNSVSHKVCARCGEALCVECRECHSKYSGQFAYCPECGAPNIE", "aa_hexdigest": "b4f73c0d4d264a03667304bbdd24628d", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 142894, "stop": 143463, "observations": { "mutation": [ 142939, 143020 ], "stop": [ 142939, 143020 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328ECB0", "query_cov": 1.0, "subject_cov": 0.7037037037037037, "identity": 0.992, "score": 266.0, "evalue": 1.22e-89, "gene_start": 1, "gene_end": 133, "reference_start": 57, "reference_end": 189, "reference_sequence": "MASGFLSGVEHIAESGVLPSTVNTVAQPQVPELVVNLQNLGSGTDVVSFISGYFPFLASYSDRELLLFGFLALVWLLCAIWTLRDAMARSDSSFYQFFSVLLIVVLTPVFGLPLYLAFRPLVYKREKGSWREALEQTVVECPHCRGLNSVSHKVCARCGEALCVECRECHSKYSGQFAYCPECGAPNIE" }, "paralog": false, "identity": 0.9735449735449735, "score": 367.0, "evalue": 1.77e-131, "description": "Internal stop codon at 142939, 143020. Nonsense mutation around 142939, 143020.", "psc": { "uniref90_id": "UniRef90_A0A328ECB0", "product": "DZANK-type domain-containing protein", "uniref50_id": "UniRef50_A0A328ECB0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328ECB0", "UniRef:UniRef50_A0A328ECB0" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328ECB0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328ECB0" ], "product": "DZANK-type domain-containing protein" } }, "id": "NKOCPLAKPK_1878", "locus": "GCA003260355_01869" }, { "type": "cds", "sequence": "contig_2", "start": 143524, "stop": 143676, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGTTGATGCTTGTGTTGATCGTAATGATTATTAGTTTAGCACTTTTGCTGGCACTTTTTTCTGGTTTTTTTCCTTTTGTGCGTACATATGGGAATGTAATGCAATATTCGACTGCCTATTATGGGGCAATTTCTGCTCTTGAAAGGTGA", "aa": "MLLMLVLIVMIISLALLLALFSGFFPFVRTYGNVMQYSTAYYGAISALER", "aa_hexdigest": "f06f98523b432c57568dd5d0069be776", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5622.8105000000005, "isoelectric_point": 8.188274574279784 }, "id": "NKOCPLAKPK_1879", "locus": "GCA003260355_01870" }, { "type": "cds", "sequence": "contig_2", "start": 144424, "stop": 144633, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAGTGCCTTGGAAACTTTTGCTAAAATTTTAACGAATGGTAATGTAAAAAATTCTTATTTAACTTTTTCTTTGGCGAATTATCTTTGGACGCAGGGAGGACATTTAGGAAGGAATCTTTATCCTTTCTTGGAATACGAGTTTCATACACTTCATGGAGTCAGGATTTCTGATAGATTTTTTACTATTCAAGGAGAAGGGAAGGTATGA", "aa": "MSALETFAKILTNGNVKNSYLTFSLANYLWTQGGHLGRNLYPFLEYEFHTLHGVRISDRFFTIQGEGKV", "aa_hexdigest": "3e64bc1beb6a7784d02e542a9cd85374", "start_type": "TTG", "rbs_motif": "TTCTA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7913.888299999999, "isoelectric_point": 8.123870658874512 }, "id": "NKOCPLAKPK_1880", "locus": "GCA003260355_01871" }, { "type": "cds", "sequence": "contig_2", "start": 144717, "stop": 144836, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGAGTCAACTTAAGCAGGTTTTCTTTTTGTCGATTTTTGGGTTTCTATGTACTGCGTTTGTCTTTTTTGTTCCACGAGGCTCTTCTTCTACCGAAGATGAGGGAGGGCAGGCTTGA", "aa": "MKSQLKQVFFLSIFGFLCTAFVFFVPRGSSSTEDEGGQA", "aa_hexdigest": "49f9ed58cd47c70b8152b7ec37e972e6", "start_type": "ATG", "rbs_motif": "TTATT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4307.898, "isoelectric_point": 5.935150337219238 }, "id": "NKOCPLAKPK_1881", "locus": "GCA003260355_01872" }, { "type": "cds", "sequence": "contig_2", "start": 144999, "stop": 145196, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCGTTTTTTGACAGGAGATGTTGTTTCTGGTGCCGATTTGCTTCTTTTGGCGTATGATGATTTTACTGGGCTTGCTCATCAATCTTTACGTTTTCAAGAGGATTTGAAGCCCTTTTTTATTCCACAATTGCGTAGCTTCTTTGATGAACAGTCAGATTTCCTTCCTGTTGCTGTGGATCCTGCGGTGATGGTTTGA", "aa": "MRFLTGDVVSGADLLLLAYDDFTGLAHQSLRFQEDLKPFFIPQLRSFFDEQSDFLPVAVDPAVMV", "aa_hexdigest": "edf8b87f6a4140e9282b7cd69dcc4f8a", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7351.324400000001, "isoelectric_point": 4.159784507751465 }, "id": "NKOCPLAKPK_1882", "locus": "GCA003260355_01873" }, { "type": "cds", "sequence": "contig_2", "start": 145515, "stop": 145904, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCCTTTTTATCCGAAAGATCCGAATTCTCAGGAAAAAATTGTTTCTTATTTGTTTCCGTACCAATATGTAGGGTTGCCTGTTAGGCTTTATGGCTTTGTGATTCCTAAAGCTACGAAAAATCGTGCAATGGTAGATCAGTGGCTTCTTGAATATATGAGTACCGCTTCAGAAAAGGGTGCTCAATTCTTGATTCCACGTATGTTTCCTGTTTTTCGTGATCAGTTTAATAAACTATGTGGGGATACAGCATGTGGATTGCCTTCTGATTTTTTTATTTTGAAAGATGGAAACGCGAAAATTCAGAGATTCCTTCAGGATAAACTGTTGAAAAAGGTGATCGAAAAAAAGATTCAACCTGACTTGTATTTAAGGGAGGCTTCTGTATAA", "aa": "MPFYPKDPNSQEKIVSYLFPYQYVGLPVRLYGFVIPKATKNRAMVDQWLLEYMSTASEKGAQFLIPRMFPVFRDQFNKLCGDTACGLPSDFFILKDGNAKIQRFLQDKLLKKVIEKKIQPDLYLREASV", "aa_hexdigest": "a05e6ba94deb713b260fc4dd9acd6529", "start_type": "GTG", "rbs_motif": "TTTA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15006.450899999982, "isoelectric_point": 9.343676948547362 }, "id": "NKOCPLAKPK_1883", "locus": "GCA003260355_01874" }, { "type": "cds", "sequence": "contig_2", "start": 145935, "stop": 146222, "strand": "+", "frame": 3, "gene": "mscL", "product": "Large conductance mechanosensitive channel protein MscL", "db_xrefs": [ "KEGG:K03282", "SO:0001217", "UniParc:UPI000DC01378", "UniRef:UniRef100_A0A660MZ29", "UniRef:UniRef50_A0A288DK55", "UniRef:UniRef90_A0A660MZ29" ], "nt": "ATGATTGAAGAATTCGCAGCGTTTTTAGCGGAGTATAATGTCGTTGGAATGGCGATAGGTCTCCTTATTGCTGTTAAAGTCGGTGATTTTGTGAAGACAATTGTTGAGGATTTAGTGACTCCATTGTTTTTTGCTCCTTTGTTTAAGAAACTCAAGATTGATAAATTGGAGGATCTTTCTCGACATGGTGTTTTGTATGGAAAGGCACTTGCTAGATTGATCGACTTTTTGATTACAGCATTTTTGGTGTTTCTTGTGATTAAGTATTTTGGTGTTGTGAAAAAGTAA", "aa": "MIEEFAAFLAEYNVVGMAIGLLIAVKVGDFVKTIVEDLVTPLFFAPLFKKLKIDKLEDLSRHGVLYGKALARLIDFLITAFLVFLVIKYFGVVKK", "aa_hexdigest": "e40a0b41469fc48cad9798e31ee53466", "start_type": "ATG", "rbs_motif": "TTATT", "ups": { "uniparc_id": "UPI000DC01378", "uniref100_id": "UniRef100_A0A660MZ29", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DC01378", "UniRef:UniRef100_A0A660MZ29" ] }, "ips": { "uniref100_id": "UniRef100_A0A660MZ29", "uniref90_id": "UniRef90_A0A660MZ29" }, "psc": { "uniref90_id": "UniRef90_A0A660MZ29", "product": "Large conductance mechanosensitive channel protein MscL", "uniref50_id": "UniRef50_A0A288DK55", "kegg_orthology_id": "K03282" }, "pscc": { "uniref50_id": "UniRef50_A0A288DK55", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A288DK55" ], "product": "Large-conductance mechanosensitive channel" }, "genes": [ "mscL" ], "id": "NKOCPLAKPK_1884", "locus": "GCA003260355_01875" }, { "type": "cds", "sequence": "contig_2", "start": 146478, "stop": 146597, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATTTTGGAAAGATGGGAGAGCTCAAGAAGATGTATGATAAGTATAAGGAGCTTCAAAAGGCACTCAAGAATTTAGTAATTAGGGCGAAAGAGGGTGAGTTTGTAGATGAAAATTGA", "aa": "MDFGKMGELKKMYDKYKELQKALKNLVIRAKEGEFVDEN", "aa_hexdigest": "86c312a8500616098cce61db3f0aff16", "start_type": "ATG", "rbs_motif": "TTATT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4638.3877, "isoelectric_point": 7.984683418273927 }, "id": "NKOCPLAKPK_1885", "locus": "GCA003260355_01876" }, { "type": "cds", "sequence": "contig_2", "start": 146637, "stop": 146831, "strand": "+", "frame": 3, "gene": null, "product": "Nucleoid-associated protein", "db_xrefs": [ "KEGG:K09747", "SO:0001217", "UniRef:UniRef50_A0A1V5ZQS7", "UniRef:UniRef50_A0A288DC82", "UniRef:UniRef90_A0A328EG65" ], "nt": "ATGAAAATTAGGGATTTTAAAGTCAATGAAATTGCTCTTCTCGATCCTGCGAAAAAAGTCGTTTTGGAAGAATTGGTGGTAAAGGCTTTTCAAAAGGCTCAAAATAAGGCTCAAGAAGTCGCAGCAGAAAAGACAAAGGATATTCTCGGTTTTGATCCTAGCAATCTGGCTGGAATGCTTGGAGGCGGAAATTGA", "aa": "MKIRDFKVNEIALLDPAKKVVLEELVVKAFQKAQNKAQEVAAEKTKDILGFDPSNLAGMLGGGN", "aa_hexdigest": "5dbeb979bdfe353fa7d28a96b1c02e17", "start_type": "ATG", "rbs_motif": "TTATT", "psc": { "uniref90_id": "UniRef90_A0A1V5ZQS7", "query_cov": 0.984375, "subject_cov": 0.9130434782608695, "identity": 0.6509999999999999, "score": 88.2, "evalue": 8.8e-22, "valid": false, "product": "YbaB/EbfC DNA-binding family", "uniref50_id": "UniRef50_A0A1V5ZQS7" }, "pscc": { "uniref50_id": "UniRef50_A0A1V5ZQS7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1V5ZQS7" ] }, "genes": [], "truncated": "both", "pseudogene": { "start": 146469, "stop": 146864, "observations": { "mutation": [ 146595, 146604, 146829 ], "stop": [ 146595, 146604, 146829 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EG65", "query_cov": 1.0, "subject_cov": 0.48854961832061067, "identity": 0.9690000000000001, "score": 121.0, "evalue": 2.85e-34, "gene_start": 1, "gene_end": 64, "reference_start": 57, "reference_end": 120, "reference_sequence": "MIVMDFGKMGELKKMYDKYKELQKALKNLVIRAKEGEFVDENGEKGSSIVIDMTGEMKIRDFKVNELALLDPAKKAVLEELVVKAFQKAQNKAQEVAAEKTKDILGFDPSNLAGMLGGGNGGGMPNIPGLS" }, "paralog": false, "identity": 0.9618320610687023, "score": 244.0, "evalue": 6.01e-85, "description": "Internal stop codon at 146595, 146604, 146829. Nonsense mutation around 146595, 146604, 146829.", "psc": { "uniref90_id": "UniRef90_A0A328EG65", "product": "Nucleoid-associated protein", "uniref50_id": "UniRef50_A0A288DC82", "kegg_orthology_id": "K09747", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EG65", "UniRef:UniRef50_A0A288DC82", "KEGG:K09747" ] }, "pscc": { "uniref50_id": "UniRef50_A0A288DC82", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A288DC82" ], "product": "Nucleoid-associated protein" } }, "id": "NKOCPLAKPK_1886", "locus": "GCA003260355_01877" }, { "type": "cds", "sequence": "contig_2", "start": 147039, "stop": 147179, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTGCTTTCGTGAAGGGAATGATCTTTATTATTTTTATCGTTCAGATGTGGTTTTTAGGATCTCTAAGCATGAGAATCAGCTTTCTTTGATTGATGAAGAGGATAAAATTGAGGTAGTTTTGCCTTTTGATCAGAAATGA", "aa": "MCFREGNDLYYFYRSDVVFRISKHENQLSLIDEEDKIEVVLPFDQK", "aa_hexdigest": "6986ab7fed6d91120465613a2fdbc765", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5630.278499999999, "isoelectric_point": 4.642290687561036 }, "id": "NKOCPLAKPK_1887", "locus": "GCA003260355_01878" }, { "type": "cds", "sequence": "contig_2", "start": 147258, "stop": 147614, "strand": "+", "frame": 3, "gene": "yprB", "product": "YprB ribonuclease H-like domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3D0UTC9" ], "nt": "ATGGATGAATTTAAAACTGGGAAATATTCTAAGATTCTTGATAAGCCTTATTTGGTTTACGATATTGAAACAACTACGATAGATGATCTAAGATCTGCAAAGTTTTTGCTTGCTTATGCTATGTTTCCTAATGGCGAAAAAATGGAATATCGTTGTATTATGCAAGATGACTTGAAGGCATTTGTAGATGAAATGCTGGCATTTGATGGCTATATCGTTGGGTTTAATCAGATTTGGTTTGATAATCCTGTGTCTGCACGGAATGTAGGGTATGGGGATCGTGAAATTGAGATCCTCAACCAAAAAAGTATTGATCTTTATGTCTTTTTTCAAACTCTTACGAAAAGGAGAATCTGA", "aa": "MDEFKTGKYSKILDKPYLVYDIETTTIDDLRSAKFLLAYAMFPNGEKMEYRCIMQDDLKAFVDEMLAFDGYIVGFNQIWFDNPVSARNVGYGDREIEILNQKSIDLYVFFQTLTKRRI", "aa_hexdigest": "c7dda7028f102abc1e3cc51a6fa85a95", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3D0UTC9", "query_cov": 0.864406779661017, "subject_cov": 0.85, "identity": 0.5, "score": 106.0, "evalue": 1.51e-27, "valid": false, "product": "YprB ribonuclease H-like domain-containing protein", "uniref50_id": "UniRef50_A0A3D0UTC9" }, "pscc": { "uniref50_id": "UniRef50_A0A3D0UTC9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3D0UTC9" ], "product": "YprB ribonuclease H-like domain-containing protein" }, "genes": [ "yprB" ], "id": "NKOCPLAKPK_1888", "locus": "GCA003260355_01879" }, { "type": "cds", "sequence": "contig_2", "start": 147762, "stop": 147902, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAGCTTTGGTAATGTTTTATTTTTTGCATTACAAGAAAATTTTTATGGATGGGGAAGAATATCCTTTTGATATTGAGGAATTTGTAGCAAAATCTCGTCATCATTCTCATGAAGAACAAGAAACTCCTGTTTTTTGA", "aa": "MTALVMFYFLHYKKIFMDGEEYPFDIEEFVAKSRHHSHEEQETPVF", "aa_hexdigest": "10644fa2c0ea3b806fdb88e19bab13f3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5655.3508, "isoelectric_point": 5.0981962203979485 }, "id": "NKOCPLAKPK_1889", "locus": "GCA003260355_01880" }, { "type": "cds", "sequence": "contig_2", "start": 147943, "stop": 148377, "strand": "+", "frame": 1, "gene": "nrdR", "product": "transcriptional regulator NrdR", "db_xrefs": [ "GO:0006355", "GO:0008270", "GO:0140110", "SO:0001217", "UniParc:UPI000DBFE7DB", "UniRef:UniRef100_A0A660MXL3", "UniRef:UniRef50_A0A3D0UT43", "UniRef:UniRef90_A0A660MXL3" ], "nt": "ATGAAATGTCCAAAGTGTAAAAATATGGATTCAAAAGTTGTGGATTCGAGGATGATTGAAGAAGGGACAATTATTAGGAGGAGAAGGGAATGTGAGAGGTGTGCCTATAGATTTACTACTTTTGAGAGGATAGGGTTGACCGAGCTCGTCGTGATCAAGAGAGATGGAACTAAAGAACTCTATGATAGAGCAAAACTTAGGAAGGCTGTTATGTTGGCTTTCGCTAAAAGACCGCTTAATGCCGAGGTTATTGATTTGATGATCAATAATTTGGAAATTTCTTGGCAGAATGCTGGATCAGAGGTTTCTAGCCAAAAAATAGGAGATGATATTTTGTCTGCGCTGAAGGAGATTGATCCTGTGGTTTACGTAAGGTTTGCTTCGGTTTATCAGTCTTTTGAAAATTTTTCCGACTTTAAGCAGTTTATTGAATAA", "aa": "MKCPKCKNMDSKVVDSRMIEEGTIIRRRRECERCAYRFTTFERIGLTELVVIKRDGTKELYDRAKLRKAVMLAFAKRPLNAEVIDLMINNLEISWQNAGSEVSSQKIGDDILSALKEIDPVVYVRFASVYQSFENFSDFKQFIE", "aa_hexdigest": "f7fcc631040e5e91aec4c7c69c05b6b7", "start_type": "ATG", "rbs_motif": "TTTTT", "ups": { "uniparc_id": "UPI000DBFE7DB", "uniref100_id": "UniRef100_A0A660MXL3", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBFE7DB", "UniRef:UniRef100_A0A660MXL3" ] }, "ips": { "uniref100_id": "UniRef100_A0A660MXL3", "uniref90_id": "UniRef90_A0A660MXL3" }, "psc": { "uniref90_id": "UniRef90_A0A660MXL3", "gene": "nrdR", "product": "transcriptional regulator NrdR", "uniref50_id": "UniRef50_A0A3D0UT43", "go_ids": [ "GO:0006355", "GO:0008270", "GO:0140110" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3D0UT43", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3D0UT43" ], "product": "Transcriptional repressor NrdR" }, "genes": [ "nrdR" ], "id": "NKOCPLAKPK_1890", "locus": "GCA003260355_01881" }, { "type": "cds", "sequence": "contig_2", "start": 148666, "stop": 149124, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGGTAAATGAATACAATATGATCGAGGAACTAGCTGTCGTCCTCCCTCCAGACACGGTAGTCAGCCTAATGAAGAAGATTCAAAAGGGAGATTTCGTCTATTATCAAATCCGCACGAGAGAATTTGATGCAGGAAGGGGTGCAAAATTTGGATATTTTCTTGATGCAAGATTCATCGAAAAAACAGCAGAAAAACCTGAAGAAAAAGCACAAACACTCCCCTCACTAGAGGACATAACAAACAAATTGCTAGCCACATCAGGTTCTGTATACGTATGGGGCGGCTCTCGATATGAAGGAATACCAGAACTTGAAAAATACTTTCCTTCAGCCACAAAACTCTCAGCCCTAGAAACCAAAAGAAAACTCATGAAAGGAGTAGACTGCTCTGGCCTTCTCTACCAAGCGACAAACGGCTTCACACCAAGAAATTCTCGTAGCCTTTTGAACTTTTGA", "aa": "MKVNEYNMIEELAVVLPPDTVVSLMKKIQKGDFVYYQIRTREFDAGRGAKFGYFLDARFIEKTAEKPEEKAQTLPSLEDITNKLLATSGSVYVWGGSRYEGIPELEKYFPSATKLSALETKRKLMKGVDCSGLLYQATNGFTPRNSRSLLNF", "aa_hexdigest": "9ed3f1e8fcef140a2c18ed4ee2e59756", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17206.57259999999, "isoelectric_point": 8.886338233947757 }, "id": "NKOCPLAKPK_1891", "locus": "GCA003260355_01882" }, { "type": "cds", "sequence": "contig_2", "start": 149389, "stop": 149613, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAACTTATCCTCTTTACGGTAGCGTTCCTAACGATGACAGGAAATGGCTGGGCATATACACCGTTTACTACAGCAGACCACCTACAAATTGATCGCCAACTCAACCAAAATCTTTCTAAAGCTGAAAAGAAAGAAAAATCTGATTTCTTTTCTCGTTTAGCCCAAAAATCTCTCGCCATTCGCAAGGCCTTCTCTCCTACAAAAACAAAGCTCTATTGA", "aa": "MKKLILFTVAFLTMTGNGWAYTPFTTADHLQIDRQLNQNLSKAEKKEKSDFFSRLAQKSLAIRKAFSPTKTKLY", "aa_hexdigest": "bed41a9ca254635a29187f74710614be", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8528.858799999995, "isoelectric_point": 10.140183830261229 }, "id": "NKOCPLAKPK_1892", "locus": "GCA003260355_01883" }, { "type": "cds", "sequence": "contig_2", "start": 149641, "stop": 149913, "strand": "-", "frame": 2, "gene": "mgsA", "product": "MgsA AAA+ ATPase C-terminal domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A9E5L583" ], "nt": "ATGCCTGAATGCGATGTTTTCCTAATGCAGCTCGCCATCTACCTTGCAAAAGCTCCGAAATCCAATCTCGCTTACAAAATTTCACTCCAAACAAAATCCGATTGTGAACGCTATGGTAATCTGCCCGTTCCTCTCCACCTAAGAAACGCCCCTACCAAGACAATGAAAACCCTCGGCTATGGTAAGGGATATCAGTATGCACACGACTTTCCCGATGCAAAAGTACAGCAAGAACACTTTCCTCCACAACTCAAAGGAAGAAAATACTCCTAA", "aa": "MPECDVFLMQLAIYLAKAPKSNLAYKISLQTKSDCERYGNLPVPLHLRNAPTKTMKTLGYGKGYQYAHDFPDAKVQQEHFPPQLKGRKYS", "aa_hexdigest": "41f4abe6adee4f7672d91f6adb9b1616", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A9E5L583", "query_cov": 0.9888888888888889, "subject_cov": 0.8725490196078431, "identity": 0.6629999999999999, "score": 125.0, "evalue": 1.51e-35, "valid": false, "product": "MgsA AAA+ ATPase C-terminal domain-containing protein", "uniref50_id": "UniRef50_A0A9E5L583" }, "pscc": { "uniref50_id": "UniRef50_A0A9E5L583", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A9E5L583" ], "product": "MgsA AAA+ ATPase C-terminal domain-containing protein" }, "genes": [ "mgsA" ], "id": "NKOCPLAKPK_1893", "locus": "GCA003260355_01884" }, { "type": "cds", "sequence": "contig_2", "start": 149914, "stop": 150048, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF12002.13" ], "nt": "ATGTTAACAGCAGGAGAGGATCCGCTCTATATCGCCAGAAGATTACTCCGCTTTGCTAGTGAAGACATAGGTCCAGCAGACAATAACGCCTTACTCCTTGCAAACCAAGTCTATGACGCAGTACAAAAAATCTGA", "aa": "MLTAGEDPLYIARRLLRFASEDIGPADNNALLLANQVYDAVQKI", "aa_hexdigest": "2bc95296539c4af85b3a9d33aeaef9e1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF12002.13", "name": "MgsA_C", "length": 44, "aa_cov": 1.0, "hmm_cov": 1.0, "evalue": 1.8539611997687782e-15, "score": 53.644569396972656, "start": 1, "stop": 44 } ], "seq_stats": { "molecular_weight": 4860.502200000002, "isoelectric_point": 4.528214836120605 }, "id": "NKOCPLAKPK_1894", "locus": "GCA003260355_01885" }, { "type": "cds", "sequence": "contig_2", "start": 150253, "stop": 150615, "strand": "-", "frame": 2, "gene": null, "product": "AAA family ATPase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A955I2E5" ], "nt": "GTGAGGAGCAAAAAAGAAGACCTGGCCACGATCATCCAACAAGCAGAACTAAACCAAAAATACGGCAAGGCAACACTAGTTTTCCTCGATGAAATCCATCGCCGAAATAAATCACAGCAAGATGCCCTTTTACCTTTTGTAGAAAAAGGGACTATCACGCTCATAGGAGCCACGACAGAAAACCCGAGTTTTACGGTAAATAACGCACTTCTTAGTCGTTGTCGCCTCTTCATTTTCGAGAAAATCAGTCCCGAAGAAATGATCAGGTTTTTTACTTTACAAAAAGGAAAAATCAGATCTCTATACCCAGAAATCCAAATCCCTTCTGAAAGCCTAAAAACCCTAGCGTCTTCCGCGAATTGA", "aa": "MRSKKEDLATIIQQAELNQKYGKATLVFLDEIHRRNKSQQDALLPFVEKGTITLIGATTENPSFTVNNALLSRCRLFIFEKISPEEMIRFFTLQKGKIRSLYPEIQIPSESLKTLASSAN", "aa_hexdigest": "0a2d3a4c8e381ad201eb60b5b8283603", "start_type": "GTG", "rbs_motif": "TTTTT", "psc": { "uniref90_id": "UniRef90_A0A955I2E5", "query_cov": 0.975, "subject_cov": 0.8731343283582089, "identity": 0.547, "score": 125.0, "evalue": 1.27e-34, "valid": false, "product": "AAA family ATPase", "uniref50_id": "UniRef50_A0A955I2E5" }, "pscc": { "uniref50_id": "UniRef50_A0A955I2E5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A955I2E5" ], "product": "AAA family ATPase" }, "genes": [], "id": "NKOCPLAKPK_1895", "locus": "GCA003260355_01886" }, { "type": "cds", "sequence": "contig_2", "start": 151087, "stop": 151527, "strand": "+", "frame": 1, "gene": null, "product": "GatB/YqeY domain-containing protein", "db_xrefs": [ "KEGG:K09117", "SO:0001217", "UniParc:UPI000DBF60F3", "UniRef:UniRef100_A0A328EGE8", "UniRef:UniRef50_A0A2W4IKJ0", "UniRef:UniRef90_A0A328EGE8" ], "nt": "ATGTTGTATGAGAAGCTCTTGGATGATTATAAATGTGCTATGAAGGAGAAGCAAGAAGTTAAAAAGTCTGCTTTAAATTTTGTTTTAGCGCAGATTAAGAATAAAAAAATTGAACTTCAGAGAGAACCTAATGATGATGAGATTATTGCTGTTCTGAAAAAAGAGGTAAAAGCCCTGAATGAGACGATTTCTTTTCTAGAGAAGGCACAAAAATCTGAAGAGTTAGCTGAAGAACAAGAGAAAAAACATGTTTTGGAATCCTATTTGCCTCAGTTGTTGGATAAGCCAACGACAAAGAAACTTATTGAAGAGGTCCTTGCACAACTTGGAATTTCTGATCTTAAGACAGGAAGAGGACTTTTGATGAAAGAATTGATGGCTAAGCACAAGAGTGAACTTGATGGGACTCTTGTAAATGAGCTTATCAATGAGATGCTTTAG", "aa": "MLYEKLLDDYKCAMKEKQEVKKSALNFVLAQIKNKKIELQREPNDDEIIAVLKKEVKALNETISFLEKAQKSEELAEEQEKKHVLESYLPQLLDKPTTKKLIEEVLAQLGISDLKTGRGLLMKELMAKHKSELDGTLVNELINEML", "aa_hexdigest": "a140245aed7d3f855f0f25d1d910c9fc", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000DBF60F3", "uniref100_id": "UniRef100_A0A328EGE8", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBF60F3", "UniRef:UniRef100_A0A328EGE8" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EGE8", "uniref90_id": "UniRef90_A0A328EGE8" }, "psc": { "uniref90_id": "UniRef90_A0A328EGE8", "product": "GatB/YqeY domain-containing protein", "uniref50_id": "UniRef50_A0A2W4IKJ0", "kegg_orthology_id": "K09117" }, "pscc": { "uniref50_id": "UniRef50_A0A2W4IKJ0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4IKJ0" ], "product": "Glutamyl-tRNA amidotransferase" }, "genes": [], "id": "NKOCPLAKPK_1896", "locus": "GCA003260355_01887" }, { "type": "cds", "sequence": "contig_2", "start": 151850, "stop": 151942, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATATTATTGAAAAAGTTGCTGTTGGAATGGCGCTTCTTGGCGCTTCTGGTGTGATCCTTAAGTTGATAAATCCGAACTTTTTTGGCTAG", "aa": "MDIIEKVAVGMALLGASGVILKLINPNFFG", "aa_hexdigest": "d219c92834e92ab0ce4ccf8f764214c7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3131.791300000001, "isoelectric_point": 5.823063850402832 }, "id": "NKOCPLAKPK_1897", "locus": "GCA003260355_01888" }, { "type": "cds", "sequence": "contig_2", "start": 152015, "stop": 152260, "strand": "+", "frame": 2, "gene": "yopX", "product": "YopX protein domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7W4E400", "UniRef:UniRef90_A0A328ECB3" ], "nt": "GTGCAGATTAGAATTGATGGAGGGTTTCAGATAGAAAAATCTTTATTTTTTGGATATGCTTATGCGCATAACGGTCTCATCAACTTGGCTTCTGAAATGGGTGCAGATATGAGGTATAATGAGGGTGAAATCTGTATTGTAGATTATCCAGGAGAGTATGATATTAGAGGATGGACGATTAAGGCTTTTGTCGGGCAGAACGCTAAGCTTAATTATTTAATCCAAGGAAATGGGAAAAAATTCTGA", "aa": "MQIRIDGGFQIEKSLFFGYAYAHNGLINLASEMGADMRYNEGEICIVDYPGEYDIRGWTIKAFVGQNAKLNYLIQGNGKKF", "aa_hexdigest": "bc53a245bfafebebdd6ec377419dac91", "start_type": "GTG", "rbs_motif": "TTATT", "genes": [ "yopX" ], "truncated": "3-prime", "pseudogene": { "start": 152009, "stop": 152410, "observations": { "mutation": [ 152258 ], "stop": [ 152258 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328ECB3", "query_cov": 1.0, "subject_cov": 0.6090225563909775, "identity": 0.988, "score": 171.0, "evalue": 1.39e-53, "gene_start": 1, "gene_end": 81, "reference_start": 3, "reference_end": 83, "reference_sequence": "MSVQIRIDGGFQIEKSLFFGYAYAHNGLINLASEMGADMRYNEGEICIVDYPGEYDIRGWTIKAFVGQNAKLNYLIQGNGKKFGIIQSSDVLELEEVDGMDTWLYLGESIEKKLDQLELEGERINLMEFSEEK" }, "paralog": false, "identity": 0.9849624060150376, "score": 266.0, "evalue": 2.58e-93, "description": "Internal stop codon at 152258. Nonsense mutation around 152258.", "psc": { "uniref90_id": "UniRef90_A0A328ECB3", "product": "YopX protein domain-containing protein", "uniref50_id": "UniRef50_A0A7W4E400", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328ECB3", "UniRef:UniRef50_A0A7W4E400" ] }, "pscc": { "uniref50_id": "UniRef50_A0A7W4E400", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7W4E400" ], "product": "YopX protein domain-containing protein" } }, "id": "NKOCPLAKPK_1898", "locus": "GCA003260355_01889" }, { "type": "cds", "sequence": "contig_2", "start": 152306, "stop": 152410, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATACATGGCTTTACCTTGGAGAATCTATTGAAAAGAAACTTGATCAGCTAGAGCTAGAAGGGGAAAGAATTAATCTTATGGAGTTTAGTGAAGAGAAGTAG", "aa": "MDTWLYLGESIEKKLDQLELEGERINLMEFSEEK", "aa_hexdigest": "cdb8c8033010a03325bee9c43e518ada", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4117.6073, "isoelectric_point": 4.2228189468383786 }, "id": "NKOCPLAKPK_1899", "locus": "GCA003260355_01890" }, { "type": "cds", "sequence": "contig_2", "start": 152436, "stop": 152636, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAAAACTCATCCCAAACTTCAAAGGGAAAATCCTACATACTGACCAGATGCAAGCCGCAGTAGATTTTGCTTTCCAATATACCGAACCAGGCCAAATCTGCCTCCTCTCTACTGCTTCACCAAGCTACAGCATCCGAAAAAATTTCGAAGAAAAAGGAAGACTCTTTCAAGACGCAATCAATAACTTCAAAGCATAA", "aa": "MAKLIPNFKGKILHTDQMQAAVDFAFQYTEPGQICLLSTASPSYSIRKNFEEKGRLFQDAINNFKA", "aa_hexdigest": "00112ebbc74dacb59f2bb0b2aeaa72f1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7483.517199999996, "isoelectric_point": 8.889626121520998 }, "id": "NKOCPLAKPK_1900", "locus": "GCA003260355_01891" }, { "type": "cds", "sequence": "contig_2", "start": 153048, "stop": 153395, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08245.18" ], "nt": "ATGACTTCACTTATTTTCTCAATCCTCAAAAATGCCGGATACAAAGTCAAGTTAGTTGGAAATATCGGAAATCCCGTTTTAGAGGAAATTAATTTTGATGAAGAATTGGATTTTGTAGTTTGCGAGCTTTCAAGCTATATGTTGGAACGTTTAGAAAAGAAAAACTATATCTCAGTACTTGGAAATATCTTCCCAGAACATATGGATCGGCACGGAGGATTTGAGAAATATGCACAGGCAAAACTGAATATTCTCAAAGGATCAGATTTTAATATTGTTTTGCAAAAAACTGCAGAAGAATATGAACTTACAAAAAGCTACACAAACATCGAAACCTATGGAATCTGA", "aa": "MTSLIFSILKNAGYKVKLVGNIGNPVLEEINFDEELDFVVCELSSYMLERLEKKNYISVLGNIFPEHMDRHGGFEKYAQAKLNILKGSDFNIVLQKTAEEYELTKSYTNIETYGI", "aa_hexdigest": "2c69326abb292b7d1dc390a74dcdd41f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08245.18", "name": "Mur_ligase_M", "length": 110, "aa_cov": 0.8, "hmm_cov": 1.0, "evalue": 6.326727787050457e-13, "score": 45.562294006347656, "start": 2, "stop": 93 } ], "seq_stats": { "molecular_weight": 13176.942599999988, "isoelectric_point": 5.015040779113768 }, "id": "NKOCPLAKPK_1901", "locus": "GCA003260355_01892" }, { "type": "cds", "sequence": "contig_2", "start": 153911, "stop": 154453, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13368.11" ], "nt": "ATGGAAAAATTTTATGGACCTTTTCACGAGCATGTCGTTGAAACCACAGGAAAAGACAGAGTAAATACTGAAAGACTCCTCGGTCAGCACCCAAGCAATGGAAAACAAATTATCGCGAGAATGGGAAGATATGGACCGATGATTCAGATTGGAGACCAAGACGATCCTGATAAAAAATTTGCTTCGATCCCGACGGGGAAAACGATTGAAACGATTACCCTTGAAGAAGCGCTACAGGCTTTTGCTCTACCAAGAAGCTTGGGAAAACGAGAAGGAGAGGAAGTTTCCGCATCTACAGGACGTTTCGGTCCTTATATAAAATACAAAAGCCTTTTTGTATCGATCAAAAAAGATTCTGGGTTAGATCCTTTTTCTATCACGCTAGAAGAAGCCATTCCGATGATTCAGGAGAAAATGGAGGCTGAGAAAAATAAATACATCAATGAATTCGACTACGAAAAGGAGAAAATTCAGGTTTTGAACGGTCCTTATGGTCCTTATATCAAATATGCCAAGAAAAATTATAAAATTCCAAAAGGCTGA", "aa": "MEKFYGPFHEHVVETTGKDRVNTERLLGQHPSNGKQIIARMGRYGPMIQIGDQDDPDKKFASIPTGKTIETITLEEALQAFALPRSLGKREGEEVSASTGRFGPYIKYKSLFVSIKKDSGLDPFSITLEEAIPMIQEKMEAEKNKYINEFDYEKEKIQVLNGPYGPYIKYAKKNYKIPKG", "aa_hexdigest": "47f8f9c146a182d33e51d5361e5fb368", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13368.11", "name": "Toprim_C_rpt", "length": 61, "aa_cov": 0.3388888888888889, "hmm_cov": 0.9016393442622951, "evalue": 1.6622043390005386e-44, "score": 146.07168579101562, "start": 30, "stop": 90 } ], "seq_stats": { "molecular_weight": 20454.1437, "isoelectric_point": 8.53866024017334 }, "id": "NKOCPLAKPK_1902", "locus": "GCA003260355_01893" }, { "type": "cds", "sequence": "contig_2", "start": 154475, "stop": 154594, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTCCGACTGATATTGGTATGATCGTAACTGACTTTTTGAATCAGAATTTTGCTGATATTATGGACTATAGTTTCACCGCAAATGTGGAACAAGAGTTTGACGAAATCGCAGAATGA", "aa": "MFPTDIGMIVTDFLNQNFADIMDYSFTANVEQEFDEIAE", "aa_hexdigest": "f271bfe0b920a1d2dcedf5903d2a0df5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4522.943899999999, "isoelectric_point": 4.0500284194946286 }, "id": "NKOCPLAKPK_1903", "locus": "GCA003260355_01894" }, { "type": "cds", "sequence": "contig_2", "start": 154760, "stop": 154936, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAATCCACAGATAATGAAGAGGATGATGAAGAAACAAAAGGAATGCTCCCTAAGCTCAGCAAAGGAGAAATGCTCCAAGAAAAAGGAATCCTCGCAACAGAAAAGTTTAAACCACATCCACCAAGATATACCGAAGCAAGTCTCGTTAAAAAGCTAGAAGAACAAGGCATCTGA", "aa": "MESTDNEEDDEETKGMLPKLSKGEMLQEKGILATEKFKPHPPRYTEASLVKKLEEQGI", "aa_hexdigest": "bc59c5ef586f7d4d85a764fa13a9f131", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6605.392, "isoelectric_point": 4.775009727478028 }, "id": "NKOCPLAKPK_1904", "locus": "GCA003260355_01895" }, { "type": "cds", "sequence": "contig_2", "start": 154952, "stop": 155335, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAGTCGCTCAGAAGCTCTATGAAGCAGGGCATATTACCTACATGAGAACCGATGCTGTGAATCTCTCTAATTTTGCGATACAGGCAGCAAAAGCCCAGATCGTCAGCGAATATGGAGAAAACTACTCCAAACCAACGCATTATGCGACCAAGGCAAAAGGAGCACAAGAAGCCCATGAATGTATCAGACCTACACATATGGAAAACCACATTGCAGGGGCAGATCCGAGCGAAAAGAAGCTCTATCAGCTGATTTGGAAAAGAACGATCGCTTCCCAGATGGCTCCCGCTGAGCTTGAAAAAACCAAGGCAACAATTCAGATTTCTACAACTGATAAGTTTCATTTTGTAGCGAGCGGAGAAGTTCTCAAGTTTGACTGA", "aa": "MQVAQKLYEAGHITYMRTDAVNLSNFAIQAAKAQIVSEYGENYSKPTHYATKAKGAQEAHECIRPTHMENHIAGADPSEKKLYQLIWKRTIASQMAPAELEKTKATIQISTTDKFHFVASGEVLKFD", "aa_hexdigest": "bf6dcf421acfc8287c5c097868f4cfac", "start_type": "ATG", "rbs_motif": "TTTTT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14228.999199999982, "isoelectric_point": 7.868124580383301 }, "id": "NKOCPLAKPK_1905", "locus": "GCA003260355_01896" }, { "type": "cds", "sequence": "contig_2", "start": 155420, "stop": 155623, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATCGTTGAGAGAGAAAGAGAAATTCAGGCTTTTGAAGCTAATTCTTTTTTCAAAATCATAGGAACATTTCTAAGCCAAGAAGAAAAAACCTTTACCGCAGAACTCAACAAACAGTTGAAATCTGAAGCTGAAACGATGAAATTCCTCGAACTTTTACAAGGTGCCAACTACGAAATCAAAGATATCGAGGTAAAACCTTGA", "aa": "MIVEREREIQAFEANSFFKIIGTFLSQEEKTFTAELNKQLKSEAETMKFLELLQGANYEIKDIEVKP", "aa_hexdigest": "0f522adcb38365af762da28df637656d", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7843.888799999997, "isoelectric_point": 4.784786033630371 }, "id": "NKOCPLAKPK_1906", "locus": "GCA003260355_01897" }, { "type": "cds", "sequence": "contig_2", "start": 155838, "stop": 156071, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCCAATTTTAGCTGGTTGGCAAGGTGTCGTCCGATCGCTTCTCCTTCACGATCCTCATCGGTTGCGATCCAGGTTTTTTCTGCTGACTCAGAGAGTCTTTTTAGCTCTGAAAGTACCTTCTTTTTGTCCGGAGAGACGACGTATTTGGGAGTAAAGCCATTGGCAATATCGATTCAAAGTTCTTTGGTCGGGAGATCTACAGTATGTCCGTATGAAGCTCTCACTTCATAG", "aa": "MSNFSWLARCRPIASPSRSSSVAIQVFSADSESLFSSESTFFLSGETTYLGVKPLAISIQSSLVGRSTVCPYEALTS", "aa_hexdigest": "661cfb9c94d7086b292c31c100f36bfa", "start_type": "ATG", "rbs_motif": "TTCTT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8245.200799999993, "isoelectric_point": 6.066391563415528 }, "id": "NKOCPLAKPK_1907", "locus": "GCA003260355_01898" }, { "type": "cds", "sequence": "contig_2", "start": 156014, "stop": 156142, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAAAAAACCTCGTAATCGTTGAGTCTCCTGCAAAATCACAGACCATAAAAAAGTTTCTCGGTCCTGACTATGAAGTGAGAGCTTCATACGGACATACTGTAGATCTCCCGACCAAAGAACTTTGA", "aa": "MAKNLVIVESPAKSQTIKKFLGPDYEVRASYGHTVDLPTKEL", "aa_hexdigest": "8caa63d1eed649870e0a92037bd894e6", "start_type": "ATG", "rbs_motif": "TTTA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4675.3617, "isoelectric_point": 8.086221122741701 }, "id": "NKOCPLAKPK_1908", "locus": "GCA003260355_01899" }, { "type": "cds", "sequence": "contig_2", "start": 156410, "stop": 156643, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATTTATCCGTTAAGCTTCCTTCTGCATTATTGGATAGGGGAAAGGTTTATCAAGGCAGTGTAGGACAGGGAGAAAAAAGAACATTAGATCAAAAAGCTGACCATGTTTTTGGACATGATTTGAATGATTTAGAAATGCCATATCACATCCGTGTTTATGTTTTTGATGAGGGAAAATCTGAACATATTGTAAGTCATAAAGGGAAATCCCTCCTTTACCCACTTGAATGA", "aa": "MNLSVKLPSALLDRGKVYQGSVGQGEKRTLDQKADHVFGHDLNDLEMPYHIRVYVFDEGKSEHIVSHKGKSLLYPLE", "aa_hexdigest": "4832a71256f57b9c1c82befd5844b0da", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8735.830999999998, "isoelectric_point": 6.499561500549316 }, "id": "NKOCPLAKPK_1909", "locus": "GCA003260355_01900" }, { "type": "cds", "sequence": "contig_2", "start": 156797, "stop": 157237, "strand": "-", "frame": 1, "gene": null, "product": "Cupin 2 conserved barrel domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EG76", "UniRef:UniRef90_A0A328EG76" ], "nt": "ATGAATAGCAATCCAAAACTCAAAGACCTTTACCAACTCATAACAGAACTCCATACTCTTGCACACAGAGAAACTCTCAATAAGCCACTTTATTTCTTCTCTAGAAGACAAAAAGCCCAAGTCGCAAAATTTCTTACAGAACTCTATTATGAGCAAAAACTTCTTTCTGAAGATACATATAAAGATATCAAAAAAAATTTGAATTTGATTTTCTTTGAAAATTTTAGCCAACAAATCCTTCCAGCAACAACAGGAATTATCGCTTCAGCATATCAAAAAAAAGAAATGTATCTCAATACCTCTAAAGTTCTAGGGAGAGATATTCTGGAACCGAGAGAATATATTGGAATCGTTCCTGAAGTAGCTTCAGATTTAGATGCTCCTTCCTTAGTAACAGCAGAAACGCCGCCAAACTATCAACAGCCATGGCACGATCACTGA", "aa": "MNSNPKLKDLYQLITELHTLAHRETLNKPLYFFSRRQKAQVAKFLTELYYEQKLLSEDTYKDIKKNLNLIFFENFSQQILPATTGIIASAYQKKEMYLNTSKVLGRDILEPREYIGIVPEVASDLDAPSLVTAETPPNYQQPWHDH", "aa_hexdigest": "12967490f7c82edef1ece09572b1c723", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 156494, "stop": 157237, "observations": { "mutation": [ 156709, 156769, 156799 ], "stop": [ 156709, 156769, 156799 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EG76", "query_cov": 1.0, "subject_cov": 0.4451219512195122, "identity": 1.0, "score": 293.0, "evalue": 4.78e-98, "gene_start": 1, "gene_end": 146, "reference_start": 1, "reference_end": 146, "reference_sequence": "MNSNPKLKDLYQLITELHTLAHRETLNKPLYFFSRRQKAQVAKFLTELYYEQKLLSEDTYKDIKKNLNLIFFENFSQQILPATTGIIASAYQKKEMYLNTSKVLGRDILEPREYIGIVPEVASDLDAPSLVTAETPPNYQQPWHDHGENREITFYTGSSISKFKTEGIEHEIQADFGDIIIFPPKTWHTIKNPTDYPVMNLSVKLPSALLDRGKVYQGSVGQGEKRTLDQKADHVFGHDLNDLEMPYHIRVYVFDEGKSEHIVSHKGKSLLYPLEGEYLATTQYFKNKTFRKGDTLLLDAETEASITNINGKGKIYTVILKENRAKQT" }, "paralog": false, "identity": 0.9878542510121457, "score": 493.0, "evalue": 7.53e-179, "description": "Internal stop codon at 156709, 156769, 156799. Nonsense mutation around 156709, 156769, 156799.", "psc": { "uniref90_id": "UniRef90_A0A328EG76", "product": "Cupin 2 conserved barrel domain-containing protein", "uniref50_id": "UniRef50_A0A328EG76", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EG76", "UniRef:UniRef50_A0A328EG76" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EG76", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EG76" ], "product": "Cupin 2 conserved barrel domain-containing protein" } }, "id": "NKOCPLAKPK_1910", "locus": "GCA003260355_01901" }, { "type": "cds", "sequence": "contig_2", "start": 158002, "stop": 159195, "strand": "-", "frame": 2, "gene": null, "product": "Glutamate--tRNA ligase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3D5YFE2" ], "nt": "TTGACAGAAGAATTTGGAAACTACGGTCCTTATGTGCAATCTCAGAGGAGGTCGATTTATGAGGTGTTTGCAAAGCATTTAGTAGCTCAAGGACTCGCCTATCCGTGCCGAATGAGCGAAGAAAGACTCAATGAAATCAGAGAAATGCAGACCGCAAGCAAGCAAATCCCCGGAATCTACGGAAACTACTCCGAATGGAGAAATAAAACTCCAGACGAACTCCTAGAAAAATTCAATGCAGAAGGTCAGACTTTTCCTGTTTTGAGGTTCCGTTCTCATGGCGATACGACCAAGAAGATCGTTTTCCAAGACCTGATCAGAGGCGAGATTTCTATGATTGACAACTATAATGATATTGTGCTAATCAAAGGAGACGGGCTTCCAACCTATCATCTTGCACATATTGTGGACGATACCTTGATGAGAGTGACGACCATCACGAGAGGTGAGGAGTGGCTGACTTCCGTTCCGCTTCATCTCCAGCTTTTCGGAGCCTTTAACTTGCCTGCACCTGAATACTGCCACTTGGCTCCGATATGTAAGCTTGATGAAGGAAAGAAAAGAAAGCTGAGCAAGAGAAAGGACCCAGAGGCAAATGTAGAATTCTTTTTTGAGGCAGGATATGCTCCACAAGCCCTTTTGGAATACATCATGACGCTCGCAGATTCTAGCTATGAAGACTGGCAAAGAGCGCATGAGAATCAGACTTTTTTGGATTTTCAGTTCTCACTTGAGAAGATGAATGTCTCAGGAGCGCTTTTTGACTTTGTGAAGCTCCAGAATATCAACAACGGCTACCTTTCCAAGCTCAACACTGCCGAACTCTATGAACAAGGACTCGCTTGGGCAAAGGACTATCACAAGGAGCTTGCCGAACTGATGGAAAAGCACGCCGAATACACCAAAGCTGCACTGAATATTGAAAGGCACACGGAAAAAGACCCGAAGAGATTTACGCTTTACAGCGATATTGAGAAAAATATCCTCTTTTTCTACGATGAGAAGCGAACTGAAATCAAAAAATCAAAACCTGACTTCCCAGAGCAGATTCCTCTTGAGACTTGGAAAGCCTTTGCTGCCGATTATGCGGAAAACTTCAGCTTAGATGGCGAGGTAACTGACCGATTTGATCAGCTTAAACTGATTGGAAAGAAATATGGTTTCGCAGGGAATAATGCGGAATTTAAGGAGTGA", "aa": "MTEEFGNYGPYVQSQRRSIYEVFAKHLVAQGLAYPCRMSEERLNEIREMQTASKQIPGIYGNYSEWRNKTPDELLEKFNAEGQTFPVLRFRSHGDTTKKIVFQDLIRGEISMIDNYNDIVLIKGDGLPTYHLAHIVDDTLMRVTTITRGEEWLTSVPLHLQLFGAFNLPAPEYCHLAPICKLDEGKKRKLSKRKDPEANVEFFFEAGYAPQALLEYIMTLADSSYEDWQRAHENQTFLDFQFSLEKMNVSGALFDFVKLQNINNGYLSKLNTAELYEQGLAWAKDYHKELAELMEKHAEYTKAALNIERHTEKDPKRFTLYSDIEKNILFFYDEKRTEIKKSKPDFPEQIPLETWKAFAADYAENFSLDGEVTDRFDQLKLIGKKYGFAGNNAEFKE", "aa_hexdigest": "d72bcd0904379a5e19d2a2044141a768", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3D5YFE2", "query_cov": 0.9949622166246851, "subject_cov": 0.9729064039408867, "identity": 0.608, "score": 504.0, "evalue": 7.26e-176, "valid": false, "product": "Glutamate--tRNA ligase", "uniref50_id": "UniRef50_A0A3D5YFE2" }, "pscc": { "uniref50_id": "UniRef50_A0A3D5YFE2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3D5YFE2" ], "product": "Glutamate--tRNA ligase" }, "genes": [], "id": "NKOCPLAKPK_1911", "locus": "GCA003260355_01902" }, { "type": "cds", "sequence": "contig_2", "start": 159379, "stop": 159507, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAACCCGAGCAGAACTCGCAGATCTCCTCTTTCCTCAAGTGAAAGAAAGCATAGATGACCTTTTGGCAAAATACCCTCCAAGACCTGAGGGGAAAGTGCTTCGTTTCGCACCAAGTCCAACCTGA", "aa": "MTTRAELADLLFPQVKESIDDLLAKYPPRPEGKVLRFAPSPT", "aa_hexdigest": "2c8dfc9cc4af9f01252f3fbfe27c5620", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4712.425100000001, "isoelectric_point": 6.061219215393066 }, "id": "NKOCPLAKPK_1912", "locus": "GCA003260355_01903" }, { "type": "cds", "sequence": "contig_2", "start": 159541, "stop": 159885, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF05746.21" ], "nt": "ATGTCTGCGAGAATTTCATCTATTTTCAAAAAAGTGGATCGTTCCCAGCTTTCAGAGCCAGATTTCAGCCTTTTGACTTTAGAAAGCGAAAAAAATCTGCTCCTTCAGCTTGCGAGTTTCCCAGACCTCGTGCAGAAATCAGCCAGCGAATATAAACCTAATGGTATTGCAAGATTTGCATTAAGCCTGGCAAAGCAGTTTTCAAGCTACTATCACGAAGCGAAAATCCGAGACGAGAACAATCTTGCTTTGAGCACGGCTCGTTTAGCCTTGATTGAAGCGATTCAGTGTGTGATGAAAAATGCTCTTGAGCTCCTCGGAATCTCGACACCTGAAAGTATGTAA", "aa": "MSARISSIFKKVDRSQLSEPDFSLLTLESEKNLLLQLASFPDLVQKSASEYKPNGIARFALSLAKQFSSYYHEAKIRDENNLALSTARLALIEAIQCVMKNALELLGISTPESM", "aa_hexdigest": "499b1a3c1b15973453ba8d8a6ca7a3a5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF05746.21", "name": "DALR_1", "length": 114, "aa_cov": 0.9912280701754386, "hmm_cov": 0.9912280701754386, "evalue": 1.5535197337111385e-31, "score": 105.33301544189453, "start": 2, "stop": 114 } ], "seq_stats": { "molecular_weight": 12695.495299999986, "isoelectric_point": 6.576350975036622 }, "id": "NKOCPLAKPK_1913", "locus": "GCA003260355_01904" }, { "type": "cds", "sequence": "contig_2", "start": 159910, "stop": 160203, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAACTCATTGGATTTGATCACGATAAATTGAAACATGTATCCTATGGTTTGGTGGATTTAGTTGATGGAAAAATGTCCTCGCGTGCAGGAAATGTCGTTTTGTATGAGGATTTCCGTGACGAACTGCTTGCAAAAGCGGAAGAGCTAGTCGCTGATCGTGATTTACCAGCAGAAAAAAAAGCTGAAATTGCTCACGATGTAGCTTTTGCTGCGATGAAATTCGGGATGCTCCTTCAAGACTCAGAAAAAGGGATCACTTTTGATAAGCAGACGGCACTTTCTTTTGAATGA", "aa": "MELIGFDHDKLKHVSYGLVDLVDGKMSSRAGNVVLYEDFRDELLAKAEELVADRDLPAEKKAEIAHDVAFAAMKFGMLLQDSEKGITFDKQTALSFE", "aa_hexdigest": "2f4802d79cfe43a6e7829a51026bfd57", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10834.217799999991, "isoelectric_point": 4.768018531799316 }, "id": "NKOCPLAKPK_1914", "locus": "GCA003260355_01905" }, { "type": "cds", "sequence": "contig_2", "start": 160243, "stop": 160572, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTGCGCTTTGGAAGGAAACAAGAGCGCTTTGTTTACAAGATATACAGCATATTTTTGCTGAGCTTGGAAGTGCAGAGATGGACAAACGATACTACGAAAGTGACGTTGAGCAGCCAGGAATCCGCATTGTTGAAGAGCTTTTGGCGAAAGGCATTGCAAAGAAATCACAGGGAGCAACGGTGATGGATTTGGAAGAATTTGGGCTTGGGGTCTTTCTTCTTCTCAAATCAACGGGAGCTTCGCTTTATTCTACCAAAGATATTGCGCTTGCCTACCAGAAAAAGTCTGATTTCCCTGACTATGATCAGTCGCTATATGTAGTTTGA", "aa": "MIALWKETRALCLQDIQHIFAELGSAEMDKRYYESDVEQPGIRIVEELLAKGIAKKSQGATVMDLEEFGLGVFLLLKSTGASLYSTKDIALAYQKKSDFPDYDQSLYVV", "aa_hexdigest": "afa71eaadcda4865862cf6c012f6c8c7", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12255.936099999988, "isoelectric_point": 4.795983314514161 }, "id": "NKOCPLAKPK_1915", "locus": "GCA003260355_01906" }, { "type": "cds", "sequence": "contig_2", "start": 160582, "stop": 161259, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03485.21" ], "nt": "ATGCTCCTTTCCTACAAAAATAAATTTGCCGAACTCCTCTCTCCATTTTTCCAAGTTTCAAAGCAGGAACTCTTGCCTCTTATTCAGATGGCTCCTGATAATGTTAAAGGAGACTTAGCTTTCCCGTGTTTTCAGTTTGCAAAGTCGCTGGAGAAGGCTCCAAATATGATTGCGAGTGAAGTGCTTGCCTTTCTTCAGGAGAAAAATCAGACTTCTTCTCTTTTTTCTGAATTTTATGCGGTAGGACCTTATGTAAATGCAAGCTTCAATACTCAAGTTCTCGCCTCAGAAGTTCTTCAGCAGATCAAGAAAGAAAAAGCTGACTTTGGAGCAGGAGAAAAGACTGGGAAAACAATCCTTCTTGAAGGTTGGGCTCCAAATACCCATAAATCCATTCATATCGGGCATGTGAGAAATTTTCTTCTAAGTGAGAGTATTGCTAGAGTTTTAAAATTCGCTGGAAATAAGGTCATCAAAACCTGCTATCCAGGCGATATTGGGGCACATGTAGCGAAATGGATTTGGTATTACAAAAACTTTGATCAGCAGCCTTTCCCAGAGCAGAATTTTACGAAATGGGTGGGACAGATCTATACTTTGGCGACGAGAAAAGTAGATGAGAATCCTAACCTTTACAAGCCTCAGATTGAAGCTCTCCAAAAGGCGCTTGAGGATTGA", "aa": "MLLSYKNKFAELLSPFFQVSKQELLPLIQMAPDNVKGDLAFPCFQFAKSLEKAPNMIASEVLAFLQEKNQTSSLFSEFYAVGPYVNASFNTQVLASEVLQQIKKEKADFGAGEKTGKTILLEGWAPNTHKSIHIGHVRNFLLSESIARVLKFAGNKVIKTCYPGDIGAHVAKWIWYYKNFDQQPFPEQNFTKWVGQIYTLATRKVDENPNLYKPQIEALQKALED", "aa_hexdigest": "206c7c8d886fe114ad29cb2f02299f20", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03485.21", "name": "Arg_tRNA_synt_N", "length": 84, "aa_cov": 0.36444444444444446, "hmm_cov": 0.9523809523809523, "evalue": 2.986754254786459e-11, "score": 40.288307189941406, "start": 5, "stop": 86 } ], "seq_stats": { "molecular_weight": 25513.057300000022, "isoelectric_point": 8.682553672790526 }, "id": "NKOCPLAKPK_1916", "locus": "GCA003260355_01907" }, { "type": "cds", "sequence": "contig_2", "start": 161579, "stop": 161734, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCGTTATCAAATCTTAAATCAAGATGCAGATTTATCTTTAATCCAAAGACTCCTAAAAGTAAGAAATGTCTCAGATGCTCCTCAAGATTTCCTCAATCCAAGTATTGCAAATTATCGGTTAGATCCTTTTTTGCTCAATGATATGGAAAAATGA", "aa": "MRYQILNQDADLSLIQRLLKVRNVSDAPQDFLNPSIANYRLDPFLLNDMEK", "aa_hexdigest": "d4d04726e99d5008909930e7396a6e8e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6006.821299999999, "isoelectric_point": 4.919437599182127 }, "id": "NKOCPLAKPK_1917", "locus": "GCA003260355_01908" }, { "type": "cds", "sequence": "contig_2", "start": 161756, "stop": 162016, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01368.27" ], "nt": "ATGAAGCGTAAGGAAAAAATTATGATTTTTGGAGATTATGATGTAGATGGGATTACTTCGAGTTTTTCGGTTTATAAATTTATTAGCTATTTTTTGGGATATAAAAATATTAGCATTAGATATCCCAACAGAGTTGAGGATGGATATGGGCTCAAAAATAAGCATTTGGATGCGATGAAAAATGAAGGAGTGCAGCTGATTATCACGGTCGATAATGGAATCGCATCCGTGGAAGAGGCAAAGTATGCTAAAGAGCTTTGA", "aa": "MKRKEKIMIFGDYDVDGITSSFSVYKFISYFLGYKNISIRYPNRVEDGYGLKNKHLDAMKNEGVQLIITVDNGIASVEEAKYAKEL", "aa_hexdigest": "d99420b815f6ac1da23492c67f5fa7fa", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01368.27", "name": "DHH", "length": 82, "aa_cov": 0.9302325581395349, "hmm_cov": 0.7439024390243902, "evalue": 1.7195777551595086e-13, "score": 47.39817810058594, "start": 6, "stop": 85 } ], "seq_stats": { "molecular_weight": 9878.230799999998, "isoelectric_point": 7.886820411682128 }, "id": "NKOCPLAKPK_1918", "locus": "GCA003260355_01909" }, { "type": "cds", "sequence": "contig_2", "start": 162023, "stop": 162118, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTGGTTACTGATCATCATCATGCTCTTGAGACGCTTCCAGATGTTTGTGCATTGATTAATCCACAGGTTTCTCCTCTCTATCCTTTTAAGTGA", "aa": "MVVTDHHHALETLPDVCALINPQVSPLYPFK", "aa_hexdigest": "2ea05716062af9fbc3f509586323cc72", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3486.025700000001, "isoelectric_point": 5.712227821350097 }, "id": "NKOCPLAKPK_1919", "locus": "GCA003260355_01910" }, { "type": "cds", "sequence": "contig_2", "start": 162134, "stop": 162334, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGCTTTTAAGCTGATTAATGCTCTTCTCTCAAAATCTACTTTTTCTAAAGAGAAAAAAAATCAGATTTTTAACTATTTTTTGCCGATTGTGGCTATTGGGACGGTGGCTGATGTGGTTCCTTTGGTCCAGGAGAATAGGGTTATTGTCAAAAGAGGCTTGGAACTTATGAATTATCATCCTGATCTTTTGCCCAAGTGA", "aa": "MAFKLINALLSKSTFSKEKKNQIFNYFLPIVAIGTVADVVPLVQENRVIVKRGLELMNYHPDLLPK", "aa_hexdigest": "6851e391dbd2941ce604b761161f75bd", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7514.892000000003, "isoelectric_point": 9.69728603363037 }, "id": "NKOCPLAKPK_1920", "locus": "GCA003260355_01911" }, { "type": "cds", "sequence": "contig_2", "start": 162533, "stop": 162790, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAGGATCAGGCTTTTAGAGTGGCAGAAAAACAGGTCAATCCTGATCAGAATTTTCTTTTTGTTTGTGATGAGTCGTTTCATGAGGGGATTGTAGGAATTGTTTCTGGGAGAATTACGGAGAAATATTACAAGCCTTCTGCTGTTTTTAAGCTTGATTTGGAGAAAAAACAGGCTGTTGCTTCCCTTAGAGGACCTGATTATTTCAATGTGATTGAGATGATTATTCAGGCTTCTCCCTATCTGAAAAGATTCTGA", "aa": "MQDQAFRVAEKQVNPDQNFLFVCDESFHEGIVGIVSGRITEKYYKPSAVFKLDLEKKQAVASLRGPDYFNVIEMIIQASPYLKRF", "aa_hexdigest": "d0f3740dc774ab8e7bec9f6798a2e3d4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9804.157199999994, "isoelectric_point": 6.556855201721191 }, "id": "NKOCPLAKPK_1921", "locus": "GCA003260355_01912" }, { "type": "cds", "sequence": "contig_2", "start": 162812, "stop": 163051, "strand": "+", "frame": 2, "gene": "recJ", "product": "RecJ OB domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A948FRD8" ], "nt": "TTGACTGTTGATCTGGAACATCTGGATACGGTGCTTGGTATTTTCCAAAACTATTGTCAGTCTCGTATTACTTCAGAACAACTGGAAAAGGTTATTCAGGTGGATACGTGTCTTCTCTCTCATGAATGGAATACAGAAGAACTTTCAGAGATTGAGCAGCTTGCTCCTTTTGGTGAGGGGAATCAAGAACCTAATTTTCTACTAGAAGGTATTAAGGTTGAAAAGGTAGAGAAGGTTTGA", "aa": "MTVDLEHLDTVLGIFQNYCQSRITSEQLEKVIQVDTCLLSHEWNTEELSEIEQLAPFGEGNQEPNFLLEGIKVEKVEKV", "aa_hexdigest": "797b2f66efeba790133bcfb96f939db2", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A948FRD8", "query_cov": 0.9493670886075949, "subject_cov": 1.0, "identity": 0.52, "score": 87.4, "evalue": 3.83e-21, "valid": false, "product": "RecJ OB domain-containing protein", "uniref50_id": "UniRef50_A0A948FRD8" }, "pscc": { "uniref50_id": "UniRef50_A0A948FRD8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A948FRD8" ], "product": "RecJ OB domain-containing protein" }, "genes": [ "recJ" ], "id": "NKOCPLAKPK_1922", "locus": "GCA003260355_01913" }, { "type": "cds", "sequence": "contig_2", "start": 163297, "stop": 164022, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF07521.17", "PFAM:PF17770.6" ], "nt": "ATGATCAACGACCTTGTTCGTCTAGGCGTAGAAGTCATTGATGAACCTGGTTTAGATACTCATACTTCAGGACATGGATACCAAGAGGATTTGAAGATGATGTTTTCTCTCTTGAATCCTGAATATTTTTGCCCAATCCACGGAGAACCTTATATGAGACATGCAAACAAAAAAGTTGCTATGATGATGGGGATCCCTGAAGATAAGGTACTTCTTCCTGATAATGGACAAATCGTAGAGCTCTACGACGAAGTAGCTTTTGTTTCAGAAAAGAGGATTAAGCTTGATACCGTAATGATTGATGGGAAAGGACAAGGGCACCTTTCTGGAGAATATGTAATGAAAGCCAGATCTATTATGGCAGAAAATGGAATCGTCGGTCTGATTTTCAAAGTTGATACCAAAACCAAAGAACTCGTCGGAAATATCCAAATTGAATCGAGAGGATTTGTCTATTCTTCAGAGGTAAAAAAGATTCATACCCAGATTGTAGAGTTCGCAAGAGCAAAATACAACGATAATTGCAAAAAGAGAATGGATATCAAGGATAATCTCAAGTTCATTAGAGAAGCACTTGGAGAATACATCACCAAAATTATCGGTAGAGTGCCAATGCTCATGCTCATGTATGTATACATCAATCGTGAAGCCGTGCAAAACATTCCAGTAGATAACAATGAAGAAATTATCGGAATGACACTCGAAGAACAGGGCTATAACGACTAA", "aa": "MINDLVRLGVEVIDEPGLDTHTSGHGYQEDLKMMFSLLNPEYFCPIHGEPYMRHANKKVAMMMGIPEDKVLLPDNGQIVELYDEVAFVSEKRIKLDTVMIDGKGQGHLSGEYVMKARSIMAENGIVGLIFKVDTKTKELVGNIQIESRGFVYSSEVKKIHTQIVEFARAKYNDNCKKRMDIKDNLKFIREALGEYITKIIGRVPMLMLMYVYINREAVQNIPVDNNEEIIGMTLEEQGYND", "aa_hexdigest": "0890cd3be35f6e8513e07a2144f27e97", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07521.17", "name": "RMMBL", "length": 39, "aa_cov": 0.15767634854771784, "hmm_cov": 1.0, "evalue": 7.613755498397648e-09, "score": 31.95015525817871, "start": 21, "stop": 58 }, { "id": "PF17770.6", "name": "RNase_J_C", "length": 98, "aa_cov": 0.4024896265560166, "hmm_cov": 1.0, "evalue": 7.692041729970778e-14, "score": 49.020668029785156, "start": 113, "stop": 209 } ], "seq_stats": { "molecular_weight": 27526.617800000036, "isoelectric_point": 5.366760444641113 }, "id": "NKOCPLAKPK_1923", "locus": "GCA003260355_01914" }, { "type": "cds", "sequence": "contig_2", "start": 164023, "stop": 164280, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF22505.1" ], "nt": "ATGGTACTTAATGTTCAGCTCTGTCAAGAACTCGGATATATCAATGTTCCTAAGGGGATGATTCGCCAGCTCTCATCAGAAGCAGAGTCTATGCCTGATGAAAAAGTACTTGTGCTTTGCACCGGTTCTCAAGGAGAAGAAAACTCAGCTCTAGTAAGAATGGCAAAAGGAGAAGTGAAAGATTTCATGCTCAGACCAGGAGATGCAGTTTTGCTTTCTTCTCACACCATTCCTGGAAATGAAAAAGCAGTAATCTGA", "aa": "MVLNVQLCQELGYINVPKGMIRQLSSEAESMPDEKVLVLCTGSQGEENSALVRMAKGEVKDFMLRPGDAVLLSSHTIPGNEKAVI", "aa_hexdigest": "b476d3162f5164a3eafcdaafc1c765df", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF22505.1", "name": "RNase_J_b_CASP", "length": 84, "aa_cov": 0.9882352941176471, "hmm_cov": 0.9880952380952381, "evalue": 1.9320676076221343e-26, "score": 88.76280212402344, "start": 1, "stop": 84 } ], "seq_stats": { "molecular_weight": 9215.631099999993, "isoelectric_point": 4.997761726379394 }, "id": "NKOCPLAKPK_1924", "locus": "GCA003260355_01915" }, { "type": "cds", "sequence": "contig_2", "start": 164476, "stop": 164994, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00753.33", "PFAM:PF12706.13" ], "nt": "ATGAATGTCGTAGAATACGGGAATGATATTTTGGTAATTGATGCTGGGATGGAGTTTGCCGACGACACAATGCCAGGTGTAGACTACATTATTCCTGATGTTAGCTATCTAGTAGCCAAAAAGAAAAATATCAAAGGAATCGTAATCACACACGGACACTTGGATCATATTGGAGCAGTAAAAAACATCATTGAAAGCCTAGGGTATCCAACAATTTACACATCTCCTCTAACAATCGGTATGATCAAGAGGAGTTTGGATGAAAAAGATGCAAAAAATCTGAAATTCAAGCTCGTTGATCCTGATATGGATATTTTCAAACTTGGAGCCTTCACTTTAGAATTTTTCAGAGTAAATCACTCAATACCAGAATCTATGGGACTTTCCCTTCATACTCCAAAAGGATTAATTGTATTCTCTGGAGATTTTAAAATTGATTTCACACCAGCGATAGATAAGCCTGCCGACCTCGGAAAAATTTCCAGAATCGGTCAAGAAGGAGTCAGACTTTACCTCTGA", "aa": "MNVVEYGNDILVIDAGMEFADDTMPGVDYIIPDVSYLVAKKKNIKGIVITHGHLDHIGAVKNIIESLGYPTIYTSPLTIGMIKRSLDEKDAKNLKFKLVDPDMDIFKLGAFTLEFFRVNHSIPESMGLSLHTPKGLIVFSGDFKIDFTPAIDKPADLGKISRIGQEGVRLYL", "aa_hexdigest": "50d273340e4e8998a67326b3980f5ee6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00753.33", "name": "Lactamase_B", "length": 149, "aa_cov": 0.8255813953488372, "hmm_cov": 0.9530201342281879, "evalue": 1.403065144975334e-14, "score": 50.932960510253906, "start": 3, "stop": 144 }, { "id": "PF12706.13", "name": "Lactamase_B_2", "length": 127, "aa_cov": 0.6511627906976745, "hmm_cov": 0.9763779527559056, "evalue": 1.446443316771355e-10, "score": 37.50585174560547, "start": 33, "stop": 144 } ], "seq_stats": { "molecular_weight": 19053.96169999999, "isoelectric_point": 5.69085636138916 }, "id": "NKOCPLAKPK_1925", "locus": "GCA003260355_01916" }, { "type": "cds", "sequence": "contig_2", "start": 165278, "stop": 165466, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAAATGAGTTTTTGACTCAACTCAGGGCTTCGTGTGAGGCGAGACGTGTCCCACTGATTTCTCGTGAAAGCGAACTTCATATCCGTAATCTCCTCCAAAAACATCAACCAAAAACTTGTCTGGAAATTGGGGCAGCTGAGGGGTATAGTAGTATCGTGATTGCAAGCACGATTGCAGAATGGTGA", "aa": "MLNEFLTQLRASCEARRVPLISRESELHIRNLLQKHQPKTCLEIGAAEGYSSIVIASTIAEW", "aa_hexdigest": "179fcdbeeea9c040d9aef7ce29bdcf79", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7023.040799999999, "isoelectric_point": 6.7117414474487305 }, "id": "NKOCPLAKPK_1926", "locus": "GCA003260355_01917" }, { "type": "cds", "sequence": "contig_2", "start": 165488, "stop": 165742, "strand": "+", "frame": 2, "gene": null, "product": "O-methyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MUH0", "UniRef:UniRef90_A0A660MUH0" ], "nt": "GTGGCGTATCCTGCCTATTTGGAAGCACAGAGAAATATTCATCAGGCAAAATGCTGGAATACGACTCTGTATCCGTTTGATATTACGAAATCTGAAACAACAGCAAGCGTATTGCCCGTGCACTGTGATTTTGTATTCATTGATGCTCAGAAAAGCCAGTATGGAACCTATTTAGAGAAAATACAAGAAAATCTTAGCTCGGAAAATATCATACTCTTGGACGATGTCGTCAAGTATCAAACAAAACTAACTTGA", "aa": "MAYPAYLEAQRNIHQAKCWNTTLYPFDITKSETTASVLPVHCDFVFIDAQKSQYGTYLEKIQENLSSENIILLDDVVKYQTKLT", "aa_hexdigest": "86e57c7d75d41b6a9a29bf682735e96b", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 165221, "stop": 165832, "observations": { "mutation": [ 165251, 165464, 165740 ], "stop": [ 165251, 165464, 165740 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A660MUH0", "query_cov": 1.0, "subject_cov": 0.41379310344827586, "identity": 0.988, "score": 172.0, "evalue": 4.92e-53, "gene_start": 1, "gene_end": 84, "reference_start": 90, "reference_end": 173, "reference_sequence": "MFDCIVVSIFGIFNLKVNKMLNEFLTQLRASCEARRVPLISRESELHIRNLLQKHQPKTCLEIGAAEGYSSIVIASTIAEWGGQLTSFEVAYPAYLEAQRNIHQAKCWNTTLYPFDITKSETTASVLPVHCDFVFIDAQKSQYGTYLEKIQENLSSENIILLDDVVKYQTKLTGLYEFLEKNQINYQILESEPGDGMMLIETK" }, "paralog": false, "identity": 0.9852216748768473, "score": 396.0, "evalue": 1.22e-143, "description": "Internal stop codon at 165251, 165464, 165740. Nonsense mutation around 165251, 165464, 165740.", "psc": { "uniref90_id": "UniRef90_A0A660MUH0", "product": "O-methyltransferase", "uniref50_id": "UniRef50_A0A660MUH0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A660MUH0", "UniRef:UniRef50_A0A660MUH0" ] }, "pscc": { "uniref50_id": "UniRef50_A0A660MUH0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MUH0" ], "product": "O-methyltransferase" } }, "id": "NKOCPLAKPK_1927", "locus": "GCA003260355_01918" }, { "type": "cds", "sequence": "contig_2", "start": 165894, "stop": 166028, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTGTTTTCTGGATGTGGTGCTAAGTCTCAGAATGAAGCTGTCGATGAGAAGCCAGAACTGGCTCCTATTTGCCCAGAAGGATTTTATTGGGTTGATTCTATCAAATCTTGCATGCTTATTTTGGATGAATAA", "aa": "MLFSGCGAKSQNEAVDEKPELAPICPEGFYWVDSIKSCMLILDE", "aa_hexdigest": "84cf1b82ba529e40e0d95b1ba0908e3a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4865.555199999999, "isoelectric_point": 4.246691322326661 }, "id": "NKOCPLAKPK_1928", "locus": "GCA003260355_01919" }, { "type": "cds", "sequence": "contig_2", "start": 166389, "stop": 166646, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03382.20" ], "nt": "GTGGCTGCTAAATGGAATACCATGGTGAATACGCTTAGTCTGAAAACAGAGGAGATTACCAAGGTGGAATTTAAGCAGGATGTTTGGTTTCCAAATGTAGCAAATAGCTTGTTCCATAGCTTTAAAAGTCAGATTATTTTTCCATCAAATCAATATACGAGCAATGTTTTGTCAATGGCAAGTATGTTTTTTGGAGCTGTAAAATTTAACTCTGATATTTCAAACTGGGATGTAAGTAATGTAACCAATATGGGCTGA", "aa": "MAAKWNTMVNTLSLKTEEITKVEFKQDVWFPNVANSLFHSFKSQIIFPSNQYTSNVLSMASMFFGAVKFNSDISNWDVSNVTNMG", "aa_hexdigest": "d3114d3880e2a1b9a7c28759e89437cd", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03382.20", "name": "DUF285", "length": 45, "aa_cov": 0.5294117647058824, "hmm_cov": 0.9333333333333333, "evalue": 6.824049454932471e-11, "score": 38.439334869384766, "start": 40, "stop": 84 } ], "seq_stats": { "molecular_weight": 9663.884499999995, "isoelectric_point": 6.5301408767700195 }, "id": "NKOCPLAKPK_1929", "locus": "GCA003260355_01920" }, { "type": "cds", "sequence": "contig_2", "start": 166938, "stop": 167108, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03382.20" ], "nt": "ATGGCTGGTATGTTTGGGAATGCAAAAAACTTCAATCAACCAATAGGAAACTGGAATACGAGTAAAGTTACTACTATGGCCTCAATGTTTTATTATGCTCATAAATTCAATCAACCAATTGGCAACTGGAATACGAGTAATGTAACTAATATGCAGTCGACCTTTCATTGA", "aa": "MAGMFGNAKNFNQPIGNWNTSKVTTMASMFYYAHKFNQPIGNWNTSNVTNMQSTFH", "aa_hexdigest": "73ca02040781b8455be86d3ff51a0698", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03382.20", "name": "DUF285", "length": 55, "aa_cov": 0.9821428571428571, "hmm_cov": 1.0, "evalue": 1.1918285050994433e-23, "score": 79.61157989501953, "start": 1, "stop": 55 } ], "seq_stats": { "molecular_weight": 6379.096600000001, "isoelectric_point": 9.824546623229981 }, "id": "NKOCPLAKPK_1930", "locus": "GCA003260355_01921" }, { "type": "cds", "sequence": "contig_2", "start": 167622, "stop": 167774, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03382.20" ], "nt": "ATGTTTGCAGGTGCTATAAAATTTAATGCTGATATTTCTAGTTGGAATACAAGCAAGGTTACTAGTATGTGGCTTATGTTTAATAGAGCTTCAAGTTTTACTCACGATATTTCAAATTGGGATTTCTCTCAAATTAATCATGGTCAATTTTGA", "aa": "MFAGAIKFNADISSWNTSKVTSMWLMFNRASSFTHDISNWDFSQINHGQF", "aa_hexdigest": "60d64750864b0732c7842c060849d60c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03382.20", "name": "DUF285", "length": 46, "aa_cov": 0.92, "hmm_cov": 1.0, "evalue": 5.2550086983326837e-17, "score": 58.16880798339844, "start": 1, "stop": 46 } ], "seq_stats": { "molecular_weight": 5775.381599999999, "isoelectric_point": 6.693609809875488 }, "id": "NKOCPLAKPK_1931", "locus": "GCA003260355_01922" }, { "type": "cds", "sequence": "contig_2", "start": 168093, "stop": 168281, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGACGCAAACTGATCTAGATAATGGAGTGCTCTCAGTAGATCCAGTAGGAACAACTGTGCAATACTCGAATTTTAACTGTAGCTTAGAACCCTTAGATGCTACTAAGGTAAACTGTAGTGTTAAGATTACTTCTACACAAGAACAAAGCGATAAAAATCTGAAACTTAAATTCTATAAGAATCTCTGA", "aa": "MTQTDLDNGVLSVDPVGTTVQYSNFNCSLEPLDATKVNCSVKITSTQEQSDKNLKLKFYKNL", "aa_hexdigest": "4370b23ba90a6f91d50725f117da03ad", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6885.671100000002, "isoelectric_point": 4.983779335021972 }, "id": "NKOCPLAKPK_1932", "locus": "GCA003260355_01923" }, { "type": "cds", "sequence": "contig_2", "start": 168960, "stop": 169142, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGACGGGAGGTGATCTTGCCTTTACGGTAATCGCTACGGATGATCAAGGAATCGCTGCTACAGGAGTTCAAATTACTTCTCCAGGTGCAGCAATGAATATCCAAAACCTTGCTTGTGTTCAGGTGAATCCTACGAAGGTGCAATGTGATTTTACGGTAAGTGATCCTGTGATTAGCTGA", "aa": "MLTGGDLAFTVIATDDQGIAATGVQITSPGAAMNIQNLACVQVNPTKVQCDFTVSDPVIS", "aa_hexdigest": "8240f7bf7ede0dee61f223718d9de58d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6109.888200000003, "isoelectric_point": 4.0500284194946286 }, "id": "NKOCPLAKPK_1933", "locus": "GCA003260355_01924" }, { "type": "cds", "sequence": "contig_2", "start": 169386, "stop": 169520, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAATTGTAGTGGATATATGAGTTTAGATGGGGTGGCTCCTGGAGCTACAGAACCCTTTACCCTCAACTTTGAAATTGATGATGCAAATCAGAATGGGAAGTATCTTTGTGTAGTAGTAAAAGATAAAGTCTGA", "aa": "MNCSGYMSLDGVAPGATEPFTLNFEIDDANQNGKYLCVVVKDKV", "aa_hexdigest": "25f6f22530ad33c0e25e81a989f5da0b", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4755.338300000002, "isoelectric_point": 4.232879447937012 }, "id": "NKOCPLAKPK_1934", "locus": "GCA003260355_01925" }, { "type": "cds", "sequence": "contig_3", "start": 105, "stop": 629, "strand": "+", "frame": 3, "gene": null, "product": "DUF1015 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A132GVQ4" ], "nt": "ATGGCAGTAATTTTCCCAGATAATCAGCTCAAAATCATGGATTACAATAGAGTTTTGAAAGATCTCAACGGAATGAGCGAAGCAGAACTGCTTGAAAAACTTCAGACTCATTTCACGGTAGAAGAAAAAGGAGAAGCTGAATATAAACCTACAGCACTTCATAATTTCAGTATGTATCTAGGCAATAAACGATACTCTCTCACTGCGAAAGAAGGAACCTATGATGATAAAGATCCTATTGGAGTGCTTGATGTGACGATCCTTTCCAATCTCGTTTTGGATCAGCTCTTCGGAATCAAAGACCTCAGAACCGACAAGAGAATTGACTTTGTAGGAGGACTTAGAGGGCTCGGAGAGCTCAAAAGAAGAGTAGATAACGGAGAGATGAAAGTTGCTTTTGCACTCTATCCAGTAAGTATGCAGCAGCTGATTGATATTGCTGACACTGGAAATATCATGCCACCAAAGACAACTTGGTTTGAGCCAAAACTCAGAAGTGGACTCATTATTCATAAGTTATCATAA", "aa": "MAVIFPDNQLKIMDYNRVLKDLNGMSEAELLEKLQTHFTVEEKGEAEYKPTALHNFSMYLGNKRYSLTAKEGTYDDKDPIGVLDVTILSNLVLDQLFGIKDLRTDKRIDFVGGLRGLGELKRRVDNGEMKVAFALYPVSMQQLIDIADTGNIMPPKTTWFEPKLRSGLIIHKLS", "aa_hexdigest": "bca3d7928279d4bd7e7a8e1022b84fe4", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A132GVQ4", "query_cov": 1.103448275862069, "subject_cov": 0.9948186528497409, "identity": 0.693, "score": 263.0, "evalue": 1.73e-87, "valid": false, "product": "DUF1015 domain-containing protein", "uniref50_id": "UniRef50_A0A132GVQ4" }, "pscc": { "uniref50_id": "UniRef50_A0A132GVQ4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A132GVQ4" ], "product": "DUF1015 domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_1935", "locus": "GCA003260355_01926" }, { "type": "cds", "sequence": "contig_3", "start": 923, "stop": 1057, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAAAGCTGAATATCTGGATACCTTTCAGCAAGTGCAAGAACTCTTGGCTACTCCTGATGAAAAAACGATTGTTGTCGTATTTACGGCAGGGGATTTGGACTATCAACTGAGGAAATCTTTTTTGCTTGGTTAA", "aa": "MKAEYLDTFQQVQELLATPDEKTIVVVFTAGDLDYQLRKSFLLG", "aa_hexdigest": "beff4c2c09fe438c7aa14c455f6c3136", "start_type": "GTG", "rbs_motif": "TTATT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5035.719299999999, "isoelectric_point": 4.385492134094237 }, "id": "NKOCPLAKPK_1936", "locus": "GCA003260355_01927" }, { "type": "cds", "sequence": "contig_3", "start": 1352, "stop": 2035, "strand": "-", "frame": 3, "gene": null, "product": "Mur ligase central domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A839JVJ0", "UniRef:UniRef90_A0A328EF08" ], "nt": "ATGAAGATTTGGAATTATTTTGAATTCCTTGGAGAGATCACGAAATATTTTCGCACCGTTGGTTTTGCTGGGACAAATGGGAAATCAAGCTCTACCTCTTTAGCGATTGTTACAGGGAAGAAACTGATTCCTGAGCTGGGACTTGGAATCGTAGGAGCGCTAGTTCCTGATCTGAATAACAAGAGTTACTATCTAAAGGAAGATAAAAAACCTGAATTTCGTCAGCTTTTTGACTTTATTTTCACAGGAAAAAAACTCCCTTATGAACTGATCAAAAAATACTATTTCTTCTTGGAAGCCTGCGAGTACAAGAGACATTTTCTCAATCTTGACCTAGAATACCTGTTGATTACCAATATTGAACTGGATCATACCGATTACTATCGTGATTTGGCTGATTATCAGTCTGCTTTCATCCAGATGAATCAAAAAATCAAAAATCTGACTTTCGTTTTGGATTCCTTTGAAGCTCCAGAAATTTTTTCTAAAATAAAGCAAATTCCCCTCAAACATTGGAATTTAGACTATATCCGAGGGAAACCGACCGATTGCAATGCTTCACTTGTCGCGGGACTTTTCCAGGAACTTAATCCTGAACTTTCTTTGGAGCAGATTGAAAAGGAAATGAAGTCATTTAAAGGGATTTGGAGGAGAATGGAGTTTTTAACGACGCTTCCAAGTTGA", "aa": "MKIWNYFEFLGEITKYFRTVGFAGTNGKSSSTSLAIVTGKKLIPELGLGIVGALVPDLNNKSYYLKEDKKPEFRQLFDFIFTGKKLPYELIKKYYFFLEACEYKRHFLNLDLEYLLITNIELDHTDYYRDLADYQSAFIQMNQKIKNLTFVLDSFEAPEIFSKIKQIPLKHWNLDYIRGKPTDCNASLVAGLFQELNPELSLEQIEKEMKSFKGIWRRMEFLTTLPS", "aa_hexdigest": "80ab6a70b8e2a4fc0d1d0028705a7b4f", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 1049, "stop": 2302, "observations": { "mutation": [ 1078, 1354, 2269, 2284 ], "stop": [ 1078, 1354, 2269, 2284 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EF08", "query_cov": 1.0, "subject_cov": 0.49455337690631807, "identity": 1.0, "score": 459.0, "evalue": 4.85e-160, "gene_start": 1, "gene_end": 227, "reference_start": 90, "reference_end": 316, "reference_sequence": "MHIVILGAGGSGISNIAHLLRDLGYTNLIAIDAQESQITQALREREIPVIIGHGKYQPKADDILIYSEATKESPEVQFAFQLKREEHFPMKIWNYFEFLGEITKYFRTVGFAGTNGKSSSTSLAIVTGKKLIPELGLGIVGALVPDLNNKSYYLKEDKKPEFRQLFDFIFTGKKLPYELIKKYYFFLEACEYKRHFLNLDLEYLLITNIELDHTDYYRDLADYQSAFIQMNQKIKNLTFVLDSFEAPEIFSKIKQIPLKHWNLDYIRGKPTDCNASLVAGLFQELNPELSLEQIEKEMKSFKGIWRRMEFLTTLPSGAKLFSDYGHIASSIQLAYETLRNRFPDHKLLAIFQPHQIHRVLKGRADFQKALAPFDQRAIFSIYTAREKFSDFADEPLFQEQHFQSFSEFGAYFAKAVKAEYLDTFQQVQELLATPDEKTIVVVFTAGDLDYQLRKSFLLG" }, "paralog": false, "identity": 0.9904076738609112, "score": 823.0, "evalue": 3.59e-306, "description": "Internal stop codon at 1078, 1354, 2269, 2284. Nonsense mutation around 1078, 1354, 2269, 2284.", "psc": { "uniref90_id": "UniRef90_A0A328EF08", "product": "Mur ligase central domain-containing protein", "uniref50_id": "UniRef50_A0A839JVJ0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EF08", "UniRef:UniRef50_A0A839JVJ0" ] }, "pscc": { "uniref50_id": "UniRef50_A0A839JVJ0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A839JVJ0" ], "product": "Mur ligase central domain-containing protein" } }, "id": "NKOCPLAKPK_1937", "locus": "GCA003260355_01928" }, { "type": "cds", "sequence": "contig_3", "start": 3012, "stop": 3302, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF04101.22" ], "nt": "TTGAAGACAACTCCAGAACTTGCAGAAATGCACTTCACGATCATTCTTGGAAAACTCAATCAAGATCTTGCTCAGCTTTTTTCTGATTTCCCGAATGTGGAGACCTTGACTTTTGTGAGTCAAAAGGAGATTGGTCGCCTTTATCTTCAGTCCGATATTGCGATCACGAGAGCTGGGACGACTTCACTTGCGGAACAGGAACTTTTTGATATGAAATTGCTGATGATTCCGATCCCACGAACTCACGACCAGTTAGACAATGCAAAGCGATATGTTGAGCATAAAAACTGA", "aa": "MKTTPELAEMHFTIILGKLNQDLAQLFSDFPNVETLTFVSQKEIGRLYLQSDIAITRAGTTSLAEQELFDMKLLMIPIPRTHDQLDNAKRYVEHKN", "aa_hexdigest": "608572f27baa01f0b478e255259cd036", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF04101.22", "name": "Glyco_tran_28_C", "length": 91, "aa_cov": 0.8958333333333334, "hmm_cov": 0.967032967032967, "evalue": 1.066987766682505e-10, "score": 38.293235778808594, "start": 9, "stop": 94 } ], "seq_stats": { "molecular_weight": 11034.58859999999, "isoelectric_point": 5.50402660369873 }, "id": "NKOCPLAKPK_1938", "locus": "GCA003260355_01929" }, { "type": "cds", "sequence": "contig_3", "start": 4298, "stop": 4411, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGACAGGATTGAAGTATCAGGTAGTTGATGGTGCGACTTATGACTATACAGTAAATGATAACTATACAAATATCGCTACTTGGGGAACTCTTCAGGTTGTATATAAGTCATAG", "aa": "MTGLKYQVVDGATYDYTVNDNYTNIATWGTLQVVYKS", "aa_hexdigest": "5acd1c830e49570ea503414c70297b68", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4193.598400000002, "isoelectric_point": 4.427894020080567 }, "id": "NKOCPLAKPK_1939", "locus": "GCA003260355_01930" }, { "type": "cds", "sequence": "contig_3", "start": 4421, "stop": 7489, "strand": "-", "frame": 3, "gene": null, "product": "SLH domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EFH7", "UniRef:UniRef90_A0A328EFH7" ], "nt": "ATGAACGTTAAAAAAATGTTCGCAGGCTTGACTGGAGGGTTAATGCTTGCATCAGTTCTTCCTGTATCAGTACTTGGTGCTGCTTCATACAGTCAGGAACTACAAGAAGCCTACAATTACGCTTATTCAAAGGGTATTACTACTATGAGCCCTATTGATAATGCTAATATGTACGGTGAACTTACTAGAGGTCAGTTGGCAAAGATGATTGCTAACTGGGCTGAAAAAGAAATGGGTACTAAAGTTGACACAACTGCTGTTTGCTCTTTCTCAGATGCACACACAGCTGAAGGAGACTTGGCTACTTATGTAAAGAAAGCTTGTCAGATGGGATTGATGGGTCAAGGAATTGACAAATTCAGACCAAATGATAAAGTAACAAGAGGTGAATTTGGAACAACTCTTTCTAGAGCTCTTTGGGGAGACAAGTACAATGGAGCTACTCCATTCTACAAGAATCATCTTGAGGCTTTGAGAGATGCTGGAATCATGAAAATGATTGATACTCCAAATCAAATGGAAATTAGAGGATATGTAATGCTTATGCTTATGAGATCAGTTAATGTAGTTACTCCTTCTGAATGTAAGGATCCTGCTGTGATCTTGGCTTGTGCATTAGGTGGTGATGCTTGTCCTGCTAGATGTAAGGGTAAGACACCAGATACTACTAATACAGGAACTAATAATACTGCTGGAGATTTGAATATCAACGTAGTTGATTACTCAAACCAGATTAGGTCTGTTCCTATGGTTGGTATTTCTATCTTCAACGCTATCAAATTTGATGCTACTAATGATGTAACTGTTAATTCAGTTACTTTGGAAAGAGTTGGTCTTTCTAGTAGATCAGACATCAAAGGTGTATGGTTCGAAAAGGATGGTATCGCTGTATCATCTAGAGGAACTGTAGCTACAGATGGAAAAGTTATGACTAACTTCAATAAAGGTTTCAAGGTTAAGTCATCAGAAACGCTTGATCTAGTTGTAGAACTTAGCGGAGCTAGCGCAGGTGCAGAAATTGCATTCAAATTTATTGGTGTAGATTCTACTGCTAAAAATGCTGTTTACAACGCTGTAACTTCAACTTATAGAACTGCTAAATATGATGTAGCTGCTCTTGATTTCTCTAATGTTGGAGGAACTGGTAATGTTGTTTACAAGCTTGGAAATCAATCAGAATTGACTTTTGGACAATTCAAGGTTTCAAATCAAGCTAAGGGAGACGACAGAAATGTATTCTTGAAGTCTGTTACTTTCAGAAACAACGGAACTGCAGACCTTAACTCTCTTCTTAAGAATGTAAAGGTTATGAGAGATAATAAAGTAGTTTCTAAGTCTGTATCTATGGATGGAAGAAACATTACTATTACTCTTGAAGATACTATCAACGCAGGAAAAGCAGTTGTTTATACTGTAATGGGTGAAGTAGCTTCTCTAGAAAGAGTTGGTGATACTGTTCAACTTGAACTTAGAAAGGATAGAGACTTGGTAGCTTATGAAGCTTCAACTAAGTTTAGAACTACTATGAATAAGCCTATTCAGGATAATTCATGGATCATGAAGAATTACAAGATCGATGGTGGTAGAGTTACTCTTACAAACACTGCTGCATTCCCTAAAACAGTTGATGCAGGAAGTGGATCTTCAGATGTTGTAATCGCAGATGGTACTCTTACTGTTGCTGAACCAATCAAGCTTCCTAAGATGACTCTTGCTGTAAATAATACAGGTGTTGTTAGATCTCTTGTTCTTGAAATCGGAGGATCTAGATATGCATCTACTGTAAATGGAACAGCTTTCGTATTTGATGATGTATATGTAAATAAGACAGCTTCTATTAAGCTTATCGCTTCATTAGAATCTTTGCCTGCACATAACTCAGTTCAGTTTGTTCCAACAACAATCGGATCATTGGCTTTCGGTTCTGCAAAGGGTGAATATACTAATAACTCAGAAGATTTGAAAGGAAATGATATCGCTGGTGCAGTACAAATTGCTAAGATTAATGTAAAAGAAGCTCAATTCACTCTTAAGAATAAAGCCTCTTCAACACAGAAAGTAACATTGACTGAATCTTCTATCAAGACAATCTTTGAAGGAGAAATGGTTGCTCCTAAGGGAGATATTAATGTAACTGAGGTAACTCTTTCTGGACTTAATGCTACTGGAATTACTGATAGTTCACAAATTGATCTTCAGCTCTATATCAATGATACTCCTGTATCTAATGCAACTTATAGAAGAGGAAACAATGTTGTAACATTCTACAATGTAGGAACTGCAACAAAAACTCCTTCTAAGTTTAGAATTGATGCTACTCCTACAATGACTCAGACTGGAGACTTCGTATTTGACGTAGTTGCTAAAGGAACTGATACTAACGGAAATACTGCAACAACTGCAGCTGTAAAGACTGTTAAATTGTCTGTAGTAGAATCTTCAAATGTATCTGTAGCTAATGCTAATGCTGTTAGCCAGGTAGTGAATGAAGGATCAAATGCTGAATTGACAAAGTTCACATTGTCTGCTAGAAATGGTTCAATCAACCTTACAGGTCTTAAGTTGAACTATAGTGGTTCCGTTGCTGCATTGAATGGACAGTCTGTGACTGTTGAAGTAAATGGAAATAGCTATACTGCTACTTCAACTGCTGCTGGTGTTGAATTCACTGGTCTTAACGTTACTCTTAACGAAGGAAATCATAATGTAGTATTCACAACGAATATTAACACTGATGGTCAAACTAATGGTGTTGCTGTTGAAGTTGCATCAGCAGAAGTGAAAGGAGACTTCCCAACAGTTACTAAGGCTATTAACTCTAAGTACTTGTTTGTGAAGGCATTCCCTAAATTGTCTGTAGTAAGTGCTAAGGATAATCAGTTGGTAGTTAAAATTGAAAATGTATCTGATGATGATATTACTATCACAGATTACGTTGCTGTTAATGCTGATCCTACGTCAACAACTCTTAATGGTCAGTCTGTTACTAATCCAATTCCAGTAGGAAAGCAGGTTACTCTTGCTAAGGGACAATCAACTGAGCTTAAGATGAATATTACTGCAGCTTGA", "aa": "MNVKKMFAGLTGGLMLASVLPVSVLGAASYSQELQEAYNYAYSKGITTMSPIDNANMYGELTRGQLAKMIANWAEKEMGTKVDTTAVCSFSDAHTAEGDLATYVKKACQMGLMGQGIDKFRPNDKVTRGEFGTTLSRALWGDKYNGATPFYKNHLEALRDAGIMKMIDTPNQMEIRGYVMLMLMRSVNVVTPSECKDPAVILACALGGDACPARCKGKTPDTTNTGTNNTAGDLNINVVDYSNQIRSVPMVGISIFNAIKFDATNDVTVNSVTLERVGLSSRSDIKGVWFEKDGIAVSSRGTVATDGKVMTNFNKGFKVKSSETLDLVVELSGASAGAEIAFKFIGVDSTAKNAVYNAVTSTYRTAKYDVAALDFSNVGGTGNVVYKLGNQSELTFGQFKVSNQAKGDDRNVFLKSVTFRNNGTADLNSLLKNVKVMRDNKVVSKSVSMDGRNITITLEDTINAGKAVVYTVMGEVASLERVGDTVQLELRKDRDLVAYEASTKFRTTMNKPIQDNSWIMKNYKIDGGRVTLTNTAAFPKTVDAGSGSSDVVIADGTLTVAEPIKLPKMTLAVNNTGVVRSLVLEIGGSRYASTVNGTAFVFDDVYVNKTASIKLIASLESLPAHNSVQFVPTTIGSLAFGSAKGEYTNNSEDLKGNDIAGAVQIAKINVKEAQFTLKNKASSTQKVTLTESSIKTIFEGEMVAPKGDINVTEVTLSGLNATGITDSSQIDLQLYINDTPVSNATYRRGNNVVTFYNVGTATKTPSKFRIDATPTMTQTGDFVFDVVAKGTDTNGNTATTAAVKTVKLSVVESSNVSVANANAVSQVVNEGSNAELTKFTLSARNGSINLTGLKLNYSGSVAALNGQSVTVEVNGNSYTATSTAAGVEFTGLNVTLNEGNHNVVFTTNINTDGQTNGVAVEVASAEVKGDFPTVTKAINSKYLFVKAFPKLSVVSAKDNQLVVKIENVSDDDITITDYVAVNADPTSTTLNGQSVTNPIPVGKQVTLAKGQSTELKMNITAA", "aa_hexdigest": "841f0800e2b67af7960b1da79dd61cbb", "start_type": "ATG", "rbs_motif": "TTTA", "psc": { "uniref90_id": "UniRef90_A0A328EFH7", "query_cov": 1.0, "subject_cov": 0.9614299153339605, "identity": 0.995, "score": 1926.0, "evalue": 0.0, "valid": true, "product": "SLH domain-containing protein", "uniref50_id": "UniRef50_A0A328EFH7" }, "pscc": { "uniref50_id": "UniRef50_A0A328EFH7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EFH7" ], "product": "SLH domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_1940", "locus": "GCA003260355_01931" }, { "type": "cds", "sequence": "contig_3", "start": 7706, "stop": 8047, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCTCCACATAGACAACTTACGAGTGATAATACCAATACCGAGATTAAAATCAAGACAAAGTTAGACGATTTCGACATTAAACTGTCAAAAGCTCACATCTGCTTAGTAAAAGCCAACCTTCAAAGAGGAAAAGCTTCCACCTGTCCTGACTTTAGAGTAGAGATACCTCACTCCTACATCCTTACACAGATGATTACGCAATTCACATTTATTTGTGACTTTTTTTTTATTTTTTTCTCACAAAAACGTAATAAAGCCAATCATAAAGCCACTTTTTACAACACAAAAACAAGCGACAAAGTCCACCGAAAACACTACAAAAGAAAAAGAAAATATTGA", "aa": "MSPHRQLTSDNTNTEIKIKTKLDDFDIKLSKAHICLVKANLQRGKASTCPDFRVEIPHSYILTQMITQFTFICDFFFIFFSQKRNKANHKATFYNTKTSDKVHRKHYKRKRKY", "aa_hexdigest": "b7df73110deaf38bbbaeab929cd8ae39", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13490.48809999998, "isoelectric_point": 10.016726875305174 }, "id": "NKOCPLAKPK_1941", "locus": "GCA003260355_01932" }, { "type": "cds", "sequence": "contig_3", "start": 8225, "stop": 8431, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAGCATTGATTGGGTATTTAGCACTTGATATGGGCTATCCTGTAATGGAGCAATTTGTCCTTGATAGTATCTACCCTGAGATTAACGAGGTTTCCAAAAGTCCGGTAAAGTCTTATAAGACGATGGCGCAGGAACATATTCAAAAATTCTATAAACACTTGCCTGTTTATAATGATTATGATGAAAAGGTTGATGAAAAGTGA", "aa": "MEALIGYLALDMGYPVMEQFVLDSIYPEINEVSKSPVKSYKTMAQEHIQKFYKHLPVYNDYDEKVDEK", "aa_hexdigest": "b8e9ea2117fddfaf20e3daf984a8227d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8021.086400000001, "isoelectric_point": 4.770178413391113 }, "id": "NKOCPLAKPK_1942", "locus": "GCA003260355_01933" }, { "type": "cds", "sequence": "contig_3", "start": 8510, "stop": 8683, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGCATATGATCATAATGAAAGACTGGAATTTGTCGGGGATGCTATGCTGGAATCGGTGATTGCGAAGAGGCTTTTCTTGGATTTCCCTGAGATGCAGGAATCTACGATGACACTTTATAAGATTGCTCTCGTAAGGGAGGAAACTCTTTGTAAAGTAGCGAAAAAAATCTGA", "aa": "MAYDHNERLEFVGDAMLESVIAKRLFLDFPEMQESTMTLYKIALVREETLCKVAKKI", "aa_hexdigest": "64ac5da2559d37a4c1a58aeea2f85117", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6666.7822, "isoelectric_point": 5.2427377700805655 }, "id": "NKOCPLAKPK_1943", "locus": "GCA003260355_01934" }, { "type": "cds", "sequence": "contig_3", "start": 8953, "stop": 9387, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTGGAGGGGCTGCGGTCTTGCTTCCTCTTGGGGCTACGGTTAATAATATTACCTATTATAAGGCGATTCATGAGTATGTGGATTTTATTTCCCATCTTCAGAGATTTCTCCTAGAGAATGCGAAAATTCAAGTCGATAATGGTATTGATAAAGAACTTCTTCGTTCAAATAAGATGCAAAATGTGAGGGTGGCCAGTAGAGTTGCTTCTAAGATGAAAAGGTATGGTTTGGATGTGATTGCTACTGACAACACAGATCCTCTTCCACTTACGAGGCTGATTATCAATACTGAAAATGCTGAGGCAAAAGGGTTTGAAGGAACTATTACAGCGATGAGGAACTTTGTGCCGATAGATGAGGTTGTGTATAATACAGGAGTGGTGAAGAAAATCATCGACGACTATGGGAATGAAGTAGAAGTGTTTACTTGA", "aa": "MFGGAAVLLPLGATVNNITYYKAIHEYVDFISHLQRFLLENAKIQVDNGIDKELLRSNKMQNVRVASRVASKMKRYGLDVIATDNTDPLPLTRLIINTENAEAKGFEGTITAMRNFVPIDEVVYNTGVVKKIIDDYGNEVEVFT", "aa_hexdigest": "81dac8a125d8d838717a004f90b10ec3", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16123.316899999983, "isoelectric_point": 5.67994327545166 }, "id": "NKOCPLAKPK_1944", "locus": "GCA003260355_01935" }, { "type": "cds", "sequence": "contig_3", "start": 9460, "stop": 9603, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTTCTGGAGGAAATCTTAGCTTGGAAAAGGCAAAAGAGCTCTATTCTGGTTATGTAGCAATGGTTAACACTAATGTTTCACTCAATGAGATGCTTTGGACAATTCAGTATTTAGATAGAGTAAAGCCGTTTTCTTTCTGA", "aa": "MLSGGNLSLEKAKELYSGYVAMVNTNVSLNEMLWTIQYLDRVKPFSF", "aa_hexdigest": "814d6433a7957a73c5eb27726021836b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5359.156, "isoelectric_point": 6.013133430480956 }, "id": "NKOCPLAKPK_1945", "locus": "GCA003260355_01936" }, { "type": "cds", "sequence": "contig_3", "start": 9922, "stop": 10140, "strand": "-", "frame": 1, "gene": null, "product": "Cell envelope-related transcriptional attenuator domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A948FPK8" ], "nt": "GTGAATGTGCTTTTGGTGGGATACGGAGGCGCTGCACATGGTGGAGGGTATTTGGCGGATTCGATCATTGTTGCTTCACGAAATCCAGAATTGGGCGCAGTGAGTATGCTTTCTATTCCGAGAGATCTCTATGTGAAGAATCCACTTGGAGGGTATTCAAGGATTAATGCGGTATTTACTCAGCTTTATGGTCGCACGAAGAGTCTGCCTGAGGCTTGA", "aa": "MNVLLVGYGGAAHGGGYLADSIIVASRNPELGAVSMLSIPRDLYVKNPLGGYSRINAVFTQLYGRTKSLPEA", "aa_hexdigest": "82399f45dd75548b2fab8bae056dfa9a", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A948FPK8", "query_cov": 0.8611111111111112, "subject_cov": 0.96875, "identity": 0.597, "score": 75.9, "evalue": 8.11e-17, "valid": false, "product": "Cell envelope-related transcriptional attenuator domain-containing protein", "uniref50_id": "UniRef50_A0A948FPK8" }, "pscc": { "uniref50_id": "UniRef50_A0A948FPK8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A948FPK8" ], "product": "Cell envelope-related transcriptional attenuator domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_1946", "locus": "GCA003260355_01937" }, { "type": "cds", "sequence": "contig_3", "start": 10144, "stop": 10269, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATCTTGGCTCTGATTCATTCTTTTGCACATACTATTAAGGGATTCTTTGTAGGAGTTGCGAAAGAGACTGTAAAAGTAGTAAGTCAGACTGTTGGGACGCCGATGAAGACAGATGCTTACTGA", "aa": "MILALIHSFAHTIKGFFVGVAKETVKVVSQTVGTPMKTDAY", "aa_hexdigest": "7d6086e1137a89eb038de94d6e72da71", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4437.229100000001, "isoelectric_point": 9.400538063049318 }, "id": "NKOCPLAKPK_1947", "locus": "GCA003260355_01938" }, { "type": "cds", "sequence": "contig_3", "start": 10776, "stop": 11705, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02896.24" ], "nt": "ATGTTCTTTGCTGAGGAAAGAATTAAGGCAGTAAGAGAGATGATCCTTGCGGAAGATGTTGAGGGAAGAAAGAAGGCGCTTGCAAAAATCCTTCCTTATCAAAAATCAGACTTTTATGAACTGATGAAAACTCTAGAAGGAATGCCAGTAACGATTAGGTTTTTGGATCCTCCTTTGCATGAATTCCTTCCTCAAGAAGAAGCTCAGATTGCAGAACTTGCTACTGAACTGGGGGTGAGTGTAGAAAGACTTCATGAGAAAATAGCTTCTCTTCATGAATTTAATCCAATGCTTGGACATAGAGGATGTAGATTGGCAATTACCTTTCCTGAAATCGCAGAAATGCAGACTGAAGCTGTGATCTCTGCCGCTTGTGAACTCAAAAAAGAAGGAGTTGATGTTCATCCAGAAATTATGATTCCACTTGTAGGATTTAAATCAGAACTAGATTTTAATGCTGACATCGTAAGAAAGGTAGCCAAAGCAACGATGGATAGATACGCTGTAGATGTGGAATACAAGCTCGGGACTATGATTGAAGTGCCAAGAGGAGCAGTAACCGCTGATAAAGTCGCAGAAACTGCGGAATTCTTCTCTTTTGGAACGAATGATCTTACTCAGATGGGACTTGGATTCTCTAGAGATGATGCAGGAAAATTTATCAAAGATTATGTAAATAAGAACATCTTTGAAAAAGATCCCTTCCAGACGCTTGATCAAGAAGGAGTAGGAGAACTCGTGAAGATCGCTGTAGAAAAAGGAAGAGCAACCAGATCAACTATCAAACTCGGAATTTGTGGAGAACACGGAGGAGATCCGGCTTCTATAGATTTCTGTCATAGAGTAGGTTTGACCTACGTAAGTTGTAGTCCGTATAGAGTGCCGATTGCAAGACTCGCTGCGGCACATGCTGCTTTGAGAAATGGATAA", "aa": "MFFAEERIKAVREMILAEDVEGRKKALAKILPYQKSDFYELMKTLEGMPVTIRFLDPPLHEFLPQEEAQIAELATELGVSVERLHEKIASLHEFNPMLGHRGCRLAITFPEIAEMQTEAVISAACELKKEGVDVHPEIMIPLVGFKSELDFNADIVRKVAKATMDRYAVDVEYKLGTMIEVPRGAVTADKVAETAEFFSFGTNDLTQMGLGFSRDDAGKFIKDYVNKNIFEKDPFQTLDQEGVGELVKIAVEKGRATRSTIKLGICGEHGGDPASIDFCHRVGLTYVSCSPYRVPIARLAAAHAALRNG", "aa_hexdigest": "7b04df3a8a3fe4bc513a3c2043d244e8", "start_type": "ATG", "rbs_motif": "TTGTA", "psc": { "uniref90_id": "UniRef90_A0A174GCR4", "query_cov": 1.0032362459546926, "subject_cov": 0.8446866485013624, "identity": 0.645, "score": 402.0, "evalue": 2.34e-137, "valid": false, "product": "Pyruvate, phosphate dikinase", "uniref50_id": "UniRef50_A0A174GCR4" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02896.24", "name": "PEP-utilizers_C", "length": 307, "aa_cov": 0.9838187702265372, "hmm_cov": 0.7850162866449512, "evalue": 4.521076272205373e-62, "score": 206.27285766601562, "start": 1, "stop": 304 } ], "seq_stats": { "molecular_weight": 34323.191800000015, "isoelectric_point": 5.415812492370605 }, "id": "NKOCPLAKPK_1948", "locus": "GCA003260355_01939" }, { "type": "cds", "sequence": "contig_3", "start": 11793, "stop": 13310, "strand": "-", "frame": 2, "gene": null, "product": "Pyruvate, phosphate dikinase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A355V105" ], "nt": "ATGGTAAGTTTAGGTTTGCCAATCCCTTCAGGATTTACTATCACGACGGAAACGTGTGATATTTACTACAAAAACAATAAAAGTTACCCAGCAGAAGTGGATCAACAGGTAGAAGAACAGCTTCAAGCGCTTGAGGCAAGGATGGGAAAGAAGCTTGGTGATGCGAATGATCCTTTGCTTGTATCTGTAAGATCTGGAGCTGCGGCCTCACTTCCAGGAATGATGGATACGGTTTTGAATCTTGGACTTAACGATGAATCTGTAGTAGGACTTGCTACCAAGACAGGAAATGAAAGGTTTGCTTGGGATTCGTATAGAAGATTTATTCAAATGTTTGGAGATGTGGTGATGGAAGTGCCTCATCATGACTTTGAAAAGGCTTTGCAATCTGTAAAAGATAAGAAAGGAGTAAAGCTGGATACAGAACTTGATACGGATGATCTCAAAGAAGTTGTAAGACTCTATAAAGAAGTCGTACAGAGAGATACAGGGAAACTTTTCCCAAATGATGGAAGACAACAGCTTAAAATGGCAATTGATGCGGTATTCAACTCATGGAATAATGAAAGAGCGATTATTTATAGAAGAATCAACGATATTAGAGGACTTCTTGGAACTGCGGTAAATGTGCAGTCTATGGTCTTTGGAAATATGGGAGAAACTTCAGGAACGGGAGTATGTTTTACAAGGAATCCTTCTACTGGGGAAAATAAATTTTACGGAGAGTTCTTGATGAATGCTCAGGGAGAAGATGTGGTAGCGGGAATCAGAACTCCAGCACATATTGAGGAGCTTGATAAGGTAATGCCTGACTGCTACAAAGAGCTTTGTGATATTTATCATTTGCTGGAAAAGCATTATAAAGATATGCAAGATATGGAGTTTACGATCCAGGAAGGAAAGCTTTATATTCTCCAGACAAGAAGTGGAAAAAGAACTGCTGCTGCGGCAGTGAAGATTGCTATGGATATGCTTGGAGAAGGACTTATCGATGAAAAGATAGCAGTACTTAGAGTACAGCCAGAGCAGCTTGATAGTTTGCTTCATCCTCAGCTTGATCCTCAGGCTAAAAAGGAAGCTGAGCTTCTTGTGAAGGCGCTTCCTGCTTCGCCAGGTGCAGCTGTTGGACAGATCGTTTTCTCTGCTGATGTTGCTCATGATTGGAAAGAAAATGGAAAGAAAGTAGTGCTTGTGAGATTAGAAACTTCACCGGAAGATATTCAAGGAATGATTGCTTCTGAAGGAATCTTGACTGCGAGAGGAGGAATGACTTCTCATGCTGCGGTAGTTGCAAGAGGAATGGGAAAATGCTGTGTCTCTGGAGCTGGAGAGCTTGTTATTGATGAAGTTGCGAAGACGGTAACTGTTCATGGAAGGACTTTCAGAGAAGGGGATCAGATTTCTCTTGATGGAGGAACGGGAGAAGTGTTTGCTGGGGCATTAAAAGTAGTGGATCCAGAACTTAGTGGAGATTTTGAGAAATTAATGCAGTTGGCGGATAAATATAGAAAACTCTGA", "aa": "MVSLGLPIPSGFTITTETCDIYYKNNKSYPAEVDQQVEEQLQALEARMGKKLGDANDPLLVSVRSGAAASLPGMMDTVLNLGLNDESVVGLATKTGNERFAWDSYRRFIQMFGDVVMEVPHHDFEKALQSVKDKKGVKLDTELDTDDLKEVVRLYKEVVQRDTGKLFPNDGRQQLKMAIDAVFNSWNNERAIIYRRINDIRGLLGTAVNVQSMVFGNMGETSGTGVCFTRNPSTGENKFYGEFLMNAQGEDVVAGIRTPAHIEELDKVMPDCYKELCDIYHLLEKHYKDMQDMEFTIQEGKLYILQTRSGKRTAAAAVKIAMDMLGEGLIDEKIAVLRVQPEQLDSLLHPQLDPQAKKEAELLVKALPASPGAAVGQIVFSADVAHDWKENGKKVVLVRLETSPEDIQGMIASEGILTARGGMTSHAAVVARGMGKCCVSGAGELVIDEVAKTVTVHGRTFREGDQISLDGGTGEVFAGALKVVDPELSGDFEKLMQLADKYRKL", "aa_hexdigest": "fa3919cfee4699e8e748c5140f57c942", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI003217F98C", "query_cov": 1.0, "subject_cov": 0.8444816053511706, "identity": 0.612, "score": 609.0, "evalue": 1.83e-212, "valid": false, "gene": "ppdK", "product": "pyruvate, phosphate dikinase", "ec_ids": [ "2.7.9.1" ], "uniref50_id": "UniRef50_A0A355V105", "go_ids": [ "GO:0006090", "GO:0050242" ] }, "pscc": { "uniref50_id": "UniRef50_A0A355V105", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A355V105" ], "product": "Pyruvate, phosphate dikinase" }, "genes": [], "id": "NKOCPLAKPK_1949", "locus": "GCA003260355_01940" }, { "type": "cds", "sequence": "contig_3", "start": 13688, "stop": 13894, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00395.25" ], "nt": "TTGGCTGGATATATTCAGCTTGCTTATCAGCTTCAGCTCATGGGAATTAAGTATGATGGAACAGCATTATCCAGCTTTGAACCAAATAAGCTTGTTACAAGGGCTGAGTTTGCTACGGTATTCTCTAGAGTCCTTTATGGAAGTAGATATAATCAGGATGGAGAAGAATGGGCGAAAGGACATCTTGAAGCTCTGAAAAATGCTTGA", "aa": "MAGYIQLAYQLQLMGIKYDGTALSSFEPNKLVTRAEFATVFSRVLYGSRYNQDGEEWAKGHLEALKNA", "aa_hexdigest": "d6a9a50f751f626232da80c647db116c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00395.25", "name": "SLH", "length": 37, "aa_cov": 0.5441176470588235, "hmm_cov": 0.8918918918918919, "evalue": 3.2862715430442926e-06, "score": 23.486963272094727, "start": 2, "stop": 38 } ], "seq_stats": { "molecular_weight": 7661.595699999999, "isoelectric_point": 6.543327522277832 }, "id": "NKOCPLAKPK_1950", "locus": "GCA003260355_01941" }, { "type": "cds", "sequence": "contig_3", "start": 13898, "stop": 14176, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTCGAACTTTGTAGAAAGTGTGTACTCAGGAGAGGTTACTTCTCCGCTTTGCTCAGTTGAAGGATCTCAGTTTTCAGAGGAACAGAATCAAGCTTACCTTTGGGCATGTGGGAAAGATATTACGACTATTAGAGAGATTTCTGGTGCGAGACTTGATCAGCCACTTACCAGAGCAGAACTTGCAAAGATGATGTCAGTGTATGTGACTAAAGTGCTAGGAAAGCAGCCTGTTGTCACAGGAACTGTTCACTATGCAGATGTTGATGAGAAGTTATGA", "aa": "MSNFVESVYSGEVTSPLCSVEGSQFSEEQNQAYLWACGKDITTIREISGARLDQPLTRAELAKMMSVYVTKVLGKQPVVTGTVHYADVDEKL", "aa_hexdigest": "ab2a1dbb0dcb2a7aadc879bdb4577fb3", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10133.370899999996, "isoelectric_point": 4.810988807678222 }, "id": "NKOCPLAKPK_1951", "locus": "GCA003260355_01942" }, { "type": "cds", "sequence": "contig_3", "start": 14184, "stop": 14297, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTATTTCCAGTACTTGATGCTGAAGATGAAGAACTTGAACTACTTGAAGATGATGATGAACTACTTGATCCTCCACCTCATCCTCCTCAACCTCCTCCTCAACAAGTATAG", "aa": "MLFPVLDAEDEELELLEDDDELLDPPPHPPQPPPQQV", "aa_hexdigest": "477cde5c091ad1165611fad5c10fc1a6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4218.600300000003, "isoelectric_point": 4.0500284194946286 }, "id": "NKOCPLAKPK_1952", "locus": "GCA003260355_01943" }, { "type": "cds", "sequence": "contig_3", "start": 14285, "stop": 14908, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF09479.16" ], "nt": "TTGGATGGGGGGACAATTAAGCTCAATGAAACTCATGCATTGATGAGAAAGGTAACAGGTGTGAGTCCTGAAATGACTGCAGTATATAATAATATCCTAGAGTTTACTGATACCACAAATCTTCTTGAAATCAAGGATGTGGCTGGAGAGAAGCTTTTGTTTGTAAATATGGCTACAGCTTGGAACAAACAGACAAAAGTATTTAGAGCAAAGAATGATGGAATTTTTGGAGCAGAAGTATTTAAATCTGCAGGAGCTAGTACAGCAACGGGTAGTATTACTCCTGATCTTGATCATGCGGTAGTTCCATCTTGGGTAGATAAGAATCATTCAATTAAGGCTTATCATGAGATTAAAAAAGATACGAAAGTGATAGGATATACTCTTCAAACAGCTAACCCTGATTATGGACGATTCTATGCGCCAAAGAAGTATAAAATTACCTTTAATGCTAATGGAGGAACTGCTGTAGCGCCAGTTGTGCAGGGATATGGAACCTCTGTTGCAGCTCCTACGAATCCTACAAGAAGCTGTTATAGATTTACTGGATGGGGTCCAGCTCTTCCAGCGACTATGCCTGCTGGAGATATTACTCTCACTGCTCAATGGGCCTATACTTGTTGA", "aa": "MDGGTIKLNETHALMRKVTGVSPEMTAVYNNILEFTDTTNLLEIKDVAGEKLLFVNMATAWNKQTKVFRAKNDGIFGAEVFKSAGASTATGSITPDLDHAVVPSWVDKNHSIKAYHEIKKDTKVIGYTLQTANPDYGRFYAPKKYKITFNANGGTAVAPVVQGYGTSVAAPTNPTRSCYRFTGWGPALPATMPAGDITLTAQWAYTC", "aa_hexdigest": "e4984daff9775eca93479741ca5377ad", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF09479.16", "name": "Flg_new", "length": 67, "aa_cov": 0.28019323671497587, "hmm_cov": 0.9701492537313433, "evalue": 6.329485646743632e-13, "score": 45.143924713134766, "start": 145, "stop": 202 } ], "seq_stats": { "molecular_weight": 22419.23179999998, "isoelectric_point": 8.95415897369385 }, "id": "NKOCPLAKPK_1953", "locus": "GCA003260355_01944" }, { "type": "cds", "sequence": "contig_3", "start": 15350, "stop": 15580, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAGAAGATAATAATACTCCAGGGTTTTATCTTTATGGGGCAAAAGATGCATTGGCTGTGTTTAGAGATGTTACTATTAATTCTGAATATAGACAACCTGTAAGCATTTCTAATCAGAAAGCAGAGTTTTATAATTCTACTATTAGTGTTGAAAAAGCTAATCCTAAAAAGTGGCTTTCTTCAGCTATTTCTGTAGATAATGATGGACAAGTTACGGTTGATGGATGA", "aa": "MKEDNNTPGFYLYGAKDALAVFRDVTINSEYRQPVSISNQKAEFYNSTISVEKANPKKWLSSAISVDNDGQVTVDG", "aa_hexdigest": "3239e9fbc41d9624d0537b2429906ec9", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8434.1992, "isoelectric_point": 4.850150871276854 }, "id": "NKOCPLAKPK_1954", "locus": "GCA003260355_01945" }, { "type": "cds", "sequence": "contig_3", "start": 15620, "stop": 15793, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCAGCTGGGATTGGATCAGAAATTGTTATAGATGGCGGAGAATATAGAGGGAATACCGATGATGCAGCTTTCTTTAGATTTAGAGGAAATGATCCAGATTCAAAAACGAAGGTTGAATTAAATAACCTTGTTGTTGAGACAAACTATCGTGTTGTTGCTAGTGATGAGTGA", "aa": "MPAGIGSEIVIDGGEYRGNTDDAAFFRFRGNDPDSKTKVELNNLVVETNYRVVASDE", "aa_hexdigest": "abd111235e0b0c18755c45329060e51d", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6250.7413000000015, "isoelectric_point": 4.429428672790528 }, "id": "NKOCPLAKPK_1955", "locus": "GCA003260355_01946" }, { "type": "cds", "sequence": "contig_3", "start": 15806, "stop": 16039, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGACTTTACAAGATGCGATAGCCTCTGCTAGTGATGGGGATACGATTCTTCTTACAAAGGATATTAATCTTAATACAGCAATATCTCTATCAAAAAATCTAACCATAGATTTAAATGGGAAAACTATTACGAATAATGTTCCACAATGGATGATGACAGTTTCATCCCCTGTAAAACTGACTCTTAAGGGAGGAAAGGTTATCACTCCAACTGCAAATACTGATTTTTGGTGA", "aa": "MTLQDAIASASDGDTILLTKDINLNTAISLSKNLTIDLNGKTITNNVPQWMMTVSSPVKLTLKGGKVITPTANTDFW", "aa_hexdigest": "be4d742edab2bd0b2ef72dfa3adc5674", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8263.428199999997, "isoelectric_point": 5.9392427444458 }, "id": "NKOCPLAKPK_1956", "locus": "GCA003260355_01947" }, { "type": "cds", "sequence": "contig_3", "start": 16316, "stop": 16849, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATAGACAAGAAAATGGTCAAAGGGTTGGCGATTATGTCGCTCGTTATGTTGCAGGCTAATATCTTTGTGCCAACAGGGATTTCACTCGCGGAGGAGGGTGAGTCTGTTGCTTCAACTGTGCAAGGAGAGAGCTTGGCAATGGAGAGCTCTTCTGTGCAAGAGGATTTAGCTTCTCCTGCTGCTTCTGCAGAGGAGAGTGTAACGGGGACGGCAGCACAGGTTTTTGAAGAAGTAGTTCAAGCTACTGGAGAAGTTGTAGATGCTTCAGGAGTGGTGATGCCATCTCTTGATGTGTTGATGTCTGCAACAGGTGCTCATATGTCTGGTGAATTGTCTGCTGGAATAATTTCTAGCTTAGGAATGAATGTAGGTGGATCTACGCTTGTGAAATATTTTACTATTGGCTCTGATGATGAGATTCCTCTTTTGATGAAAGATGATGGAACGGTTGATTATACCGGTACTAAATTGCAATATTCTGATACAACGGATTATTATTTGAATACGAAGTCTCAAGTGGTTGATTTTTGA", "aa": "MIDKKMVKGLAIMSLVMLQANIFVPTGISLAEEGESVASTVQGESLAMESSSVQEDLASPAASAEESVTGTAAQVFEEVVQATGEVVDASGVVMPSLDVLMSATGAHMSGELSAGIISSLGMNVGGSTLVKYFTIGSDDEIPLLMKDDGTVDYTGTKLQYSDTTDYYLNTKSQVVDF", "aa_hexdigest": "335f7a629d4e80f5c34521f2e64a6a17", "start_type": "ATG", "rbs_motif": "TTATT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18422.5529, "isoelectric_point": 4.0500284194946286 }, "id": "NKOCPLAKPK_1957", "locus": "GCA003260355_01948" }, { "type": "cds", "sequence": "contig_3", "start": 17103, "stop": 17648, "strand": "+", "frame": 3, "gene": null, "product": "Peptidase M10 metallopeptidase domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EGY0", "UniRef:UniRef90_A0A328EGY0" ], "nt": "ATGAAAAAGAAATACCTATCCATTCTCCTCTTAGGAACATTACTTCTCCATTTCGGAATAGGAAAAGCAGAAAATTCAGATCAAAAATTAGATTCTATCTTCGAAATCCAAGACTACGTAGAACAAACACTTAATATCAAATCCTCAACAATCTCAAGCAGAAACTATTTCCCTCTTATCAAAGAAATCTTAGAGAAAAAGTGCCCCTCCGAGAGCATAGATGAAGCCAAAAGTACTCTTGAACAAACCCTCCCTACTCGACAAGAGCTAATAAGCCAGATTTCTCCTAATAGAATCAAGTATGAATTTGAAAAAATCAAAACCTCAACATTGCAAATCCAAACAGAAAACCCTACCGAATTTTGCAAAAAAAAATACATCAGTTATACCTTCCTAGAAATGATGAGAAATCAGAATCTTTCTCACTACCAAAATGAACAAGAACACCCAGCTGCCAACCCTAGACAATCGGAAAGAGAAAATTCCACTTCTCAAAACCAACAACCAACAATAGAAGTCCAAAACAATACAAAAAACCTCTCATGA", "aa": "MKKKYLSILLLGTLLLHFGIGKAENSDQKLDSIFEIQDYVEQTLNIKSSTISSRNYFPLIKEILEKKCPSESIDEAKSTLEQTLPTRQELISQISPNRIKYEFEKIKTSTLQIQTENPTEFCKKKYISYTFLEMMRNQNLSHYQNEQEHPAANPRQSERENSTSQNQQPTIEVQNNTKNLS", "aa_hexdigest": "f68ea321986d3a89168a6a9cc48c0480", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 17103, "stop": 17951, "observations": { "mutation": [ 17646, 17781 ], "stop": [ 17646, 17781 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EGY0", "query_cov": 1.0, "subject_cov": 0.49725274725274726, "identity": 0.983, "score": 330.0, "evalue": 1.7e-111, "gene_start": 1, "gene_end": 181, "reference_start": 1, "reference_end": 181, "reference_sequence": "MKKKYLSILLLGTLLLDFGIGKAENSDQKLDSIFEIQDYVEQTLNIKSSTISSRNYFPLIKEILEKKCPSESVDEAKSTLEQTLPTRQELISQISPNRIKYEFEKIKTSTLQIQTENPTEFCKKKYISYTFLEMMRNQNLSHYQNEQEHPAANPRQSERENSASQNQQPTIEVQNNTKNLSGEDLTFAEQTKQFAHEELTNLLSLGILNPHDQETLNNHIEINYLPGCKKTHGSFHILQNKTTKEKKFKTLKLNIAICNTAEYQANYRAYFKQILAHELGHYIYFFRDQNSQAFDTICRNNEQNTCNSKGFFSEYAQKSKEEDYAESFAYRYKNLEKEKEFGSAPTSETIYQKENHFNQLFASH" }, "paralog": false, "identity": 0.975177304964539, "score": 550.0, "evalue": 3.25e-200, "description": "Internal stop codon at 17646, 17781. Nonsense mutation around 17646, 17781.", "psc": { "uniref90_id": "UniRef90_A0A328EGY0", "product": "Peptidase M10 metallopeptidase domain-containing protein", "uniref50_id": "UniRef50_A0A328EGY0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EGY0", "UniRef:UniRef50_A0A328EGY0" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EGY0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EGY0" ], "product": "Peptidase M10 metallopeptidase domain-containing protein" } }, "id": "NKOCPLAKPK_1958", "locus": "GCA003260355_01949" }, { "type": "cds", "sequence": "contig_3", "start": 18574, "stop": 18984, "strand": "-", "frame": 1, "gene": null, "product": "ComEC/Rec2-related protein domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328ECX9", "UniRef:UniRef90_A0A660MT79" ], "nt": "ATGGATACTTTTGCTTTAAAGGTTATTTCTGGTAGTTTCCTTTTAGGAAAGTTGCCAAGCCTTGGTCAGAGAATTGCTTCTTCTGGATGTGTTCTTTCTAGTGGAGTTTTTAATTTTCCCGAGGTTAAGTATGAAGAAAAATCGAAGTTTTCCGATCTCCTGGTGGTCGTTTCCAATCTCTTTTGTCTTGGAGAAGTTTCGATTAAATCAGGTTTGCTGCGAAAGGTTTCTATGGCTCTTTTGGGAGAGCGAGTGATGATTATCCTTCCTGAGCGATGGTTTCAAGATGCGAAAACTCGATATATCGTTGCTGATGATCGTGGAAGTCTCTGGCTTTTGCATTCATTAACGATGGAAATGAGTAATGCGATCCAGCATCCTGTCTCATACTTTGTCGTTCCACAGGTATGA", "aa": "MDTFALKVISGSFLLGKLPSLGQRIASSGCVLSSGVFNFPEVKYEEKSKFSDLLVVVSNLFCLGEVSIKSGLLRKVSMALLGERVMIILPERWFQDAKTRYIVADDRGSLWLLHSLTMEMSNAIQHPVSYFVVPQV", "aa_hexdigest": "87ae9a9db26ffbcba1d9804c48ec33ab", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 18358, "stop": 18984, "observations": { "mutation": [ 18462, 18498, 18510, 18576 ], "stop": [ 18462, 18498, 18510, 18576 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A660MT79", "query_cov": 1.0, "subject_cov": 0.6538461538461539, "identity": 0.912, "score": 243.0, "evalue": 7.25e-80, "gene_start": 1, "gene_end": 136, "reference_start": 1, "reference_end": 136, "reference_sequence": "MHTFALKVISGSFPIGKLPSLGRRIASSRCVLSSGVFNFPEVKYEEKSKFSDLLVVVSNLFCLGKVPIKSDFLRNVSMALLGEQVMIILPERWFQDAKTRYIVADDRRSLWLLHSLTMEMSNAIQHPVSYFVVPQVGDYCFEGIPSLILGVSFFCVCSGFSLGYLHSDVLFYLFGGNVFFDLRMFVVCFAAFLWLFFVLFWRKCPFSF" }, "paralog": false, "identity": 0.9038461538461539, "score": 372.0, "evalue": 4.09e-133, "description": "Internal stop codon at 18462, 18498, 18510, 18576. Nonsense mutation around 18462, 18498, 18510, 18576.", "psc": { "uniref90_id": "UniRef90_A0A660MT79", "product": "ComEC/Rec2-related protein domain-containing protein", "uniref50_id": "UniRef50_A0A328ECX9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A660MT79", "UniRef:UniRef50_A0A328ECX9" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328ECX9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328ECX9" ], "product": "ComEC/Rec2-related protein domain-containing protein" } }, "id": "NKOCPLAKPK_1959", "locus": "GCA003260355_01950" }, { "type": "cds", "sequence": "contig_3", "start": 19073, "stop": 19255, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGATTCAGACTTCTTTTTTTGATCAATCTCTACAGAATTATCAAGCAGTTCCAGAAAGAGAAGTAGTTCCAGAAAGAGATACAGCTCCAGATGTTACATCTTCCTCAGAGAAATCATCAAACTCTCCTGAAAATCCTCACATAGCTCCACCTCCCATTAATGCACTCCAAATTTCTTGA", "aa": "MKIQTSFFDQSLQNYQAVPEREVVPERDTAPDVTSSSEKSSNSPENPHIAPPPINALQIS", "aa_hexdigest": "3dc8e1b680342ae6ead3a0d2c4b63440", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6624.1862, "isoelectric_point": 4.546744346618653 }, "id": "NKOCPLAKPK_1960", "locus": "GCA003260355_01951" }, { "type": "cds", "sequence": "contig_3", "start": 19213, "stop": 19392, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAGTCAAAATCGTTTCTCTCTTCAACCTCAGTTTAAATTGACTATAGATAAAGTAGGAGTAGATGTAGACGGAGATTTCGTTGCAAAGAAAATTGCTAATCATACTTTTACTACGCCTACTGATGCTCAGAATCTTCAAGAAATTTGGAGTGCATTAATGGGAGGTGGAGCTATGTGA", "aa": "MSQNRFSLQPQFKLTIDKVGVDVDGDFVAKKIANHTFTTPTDAQNLQEIWSALMGGGAM", "aa_hexdigest": "44789960130171cf095a13b0407610cc", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6470.2824, "isoelectric_point": 5.485894966125487 }, "id": "NKOCPLAKPK_1961", "locus": "GCA003260355_01952" }, { "type": "cds", "sequence": "contig_3", "start": 19483, "stop": 19812, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCTTGATTTCTATGCTTTGAATGAGACTGGAGAAGTGGCTTCTACTGGAAATGAAGTATTGCTTGCTCAACAACAGCAGATGAAAGACAGTTTGGATCAGATTTTTTCTAGTATAAAATTTGAGAATACTGAAGCTTATTTTGTGATTCGTTCTGCAGATAAGGTGGATTTTGTAGTAGTAAATAGTGATTTCTCAATGGGTGATCTTAAAATGAAGCTTAGTCAGCACGTAAAAGGTAAGGTAACGACAGCTAAGGCAATTATTCCATCTGCTGATGGATCTGGACAAGTGGAAGTAACCCTTGTTGCTGAGAGAGCTGGTATCTGA", "aa": "MLDFYALNETGEVASTGNEVLLAQQQQMKDSLDQIFSSIKFENTEAYFVIRSADKVDFVVVNSDFSMGDLKMKLSQHVKGKVTTAKAIIPSADGSGQVEVTLVAERAGI", "aa_hexdigest": "4b9fffc2fcd32732fa0d05e19eedecf5", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11863.317699999989, "isoelectric_point": 4.699129676818846 }, "id": "NKOCPLAKPK_1962", "locus": "GCA003260355_01953" }, { "type": "cds", "sequence": "contig_3", "start": 19861, "stop": 20376, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTATAATAAACAGAATGCTATCGTAAAGGATGTATTACAGTTTATGGGTGATCAGGAGAAGCTAATTGAATCAAATCTTAAAGCACAGGTAGATGTGCAGACTGATGGAGGTAAAGGAAAAGTTATGCTTGATGTGAAGAGTGTATCAGATAATAAGACGAAAGATTCTGAGTCTGACATAGGTCTTAATCTTACTATGGAGCAGAAAGATGCTCAAACAGGAGACCTTCAGAAATTGGATGCGAAAGTGAATTTGAATACGCTTCTCAAGGATTATCAGCTCTATTTTAAGCTTAATGAGTTTGCTTTGGAGATGAATCCAAAAGAGGCTGCAGGAATGCCGTTAGCGATGGTTGAAATGTTTAAGAATAAGTGGCTTACTTTGGATGTTCCAGAAATTAAGGAAATGCTTAGGACTTCAGCAGAGACTAATGCTAATATTTGGCAGAATGAAAAACTTTATGCAGTGAATCCAAGTTATTATACGGGAGTGGTATCTACTACTTATGAATGA", "aa": "MYNKQNAIVKDVLQFMGDQEKLIESNLKAQVDVQTDGGKGKVMLDVKSVSDNKTKDSESDIGLNLTMEQKDAQTGDLQKLDAKVNLNTLLKDYQLYFKLNEFALEMNPKEAAGMPLAMVEMFKNKWLTLDVPEIKEMLRTSAETNANIWQNEKLYAVNPSYYTGVVSTTYE", "aa_hexdigest": "27d787a83d04ce1acf041fc02d4d97b5", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19386.887500000008, "isoelectric_point": 4.841795539855956 }, "id": "NKOCPLAKPK_1963", "locus": "GCA003260355_01954" }, { "type": "cds", "sequence": "contig_3", "start": 20968, "stop": 21201, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGACGAACCAGCTTTTTCTTTCTTTTCTTTCTCAATTTAGGGGAGCTGATGCTGATTTATATTTTCATCCGGAAAGGTTTCGTTTTTCTCAGGGAGAGGGGAGTGTAAATTCACAATTTGTTCAATTGATTGCGAAAGATCTCCTTCATTATTGGATAAAAATTCCATGGAATAAAGATGTTTTTGTATTCCCTTCTAAGGAATTACTTTTATTTCCACAGATTTTTTCTTGA", "aa": "MTNQLFLSFLSQFRGADADLYFHPERFRFSQGEGSVNSQFVQLIAKDLLHYWIKIPWNKDVFVFPSKELLLFPQIFS", "aa_hexdigest": "dac24874281863be748878a24e92b211", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9145.412599999996, "isoelectric_point": 6.708046913146972 }, "id": "NKOCPLAKPK_1964", "locus": "GCA003260355_01955" }, { "type": "cds", "sequence": "contig_3", "start": 21241, "stop": 21390, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATCAAATGATTTTTTTGATAAATGAGGCTTTTGCTGGAGAAAAATCAGTCTTTTCTGGTGACGGTCAGCTGCTTGCTTCTACGAAATCTCGCGTAGTAAGTGTAAATTTAACTGGTTTTGTAGCGTATTCTGGTCAAGAATTTTGA", "aa": "MNQMIFLINEAFAGEKSVFSGDGQLLASTKSRVVSVNLTGFVAYSGQEF", "aa_hexdigest": "40cde86cd24d80226eed88d0b213a0b3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5289.9449, "isoelectric_point": 4.867827796936035 }, "id": "NKOCPLAKPK_1965", "locus": "GCA003260355_01956" }, { "type": "cds", "sequence": "contig_3", "start": 21661, "stop": 21966, "strand": "+", "frame": 1, "gene": null, "product": "Bacterial type II secretion system protein E domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328ECY0", "UniRef:UniRef90_A0A328ECY0" ], "nt": "ATGAACACAGTAAGAGTCGATATTGATTCCATTTTAGCCGGGATTGCAGAAAACCCGAATATTTCCGACCTTCATCTAACCTGCTCCGGAAAAAGTTCATATAGACTAAACGGGGATATTATCAAAGATGAAAAAATTCCGATCATCAATAGCGAAAATATGGAAATCATCCTCAAACAACTCTTTCAAAATAATGCCCAAAATTTTGATAAGTTTATCTGCGACAAAGAAAGCGATTTCGCATATGAAGGGAAAGATGGAACCTCTTATAGAGTAAATGCCTTTTTTGAAAAAGGAAAAATCTGA", "aa": "MNTVRVDIDSILAGIAENPNISDLHLTCSGKSSYRLNGDIIKDEKIPIINSENMEIILKQLFQNNAQNFDKFICDKESDFAYEGKDGTSYRVNAFFEKGKI", "aa_hexdigest": "dc748df2d1bb779a1671622ea70ca68f", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 21661, "stop": 22208, "observations": { "deletion": [ 22063 ], "mutation": [ 21964 ], "stop": [ 21964, 22132, 22186, 22198 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328ECY0", "query_cov": 1.0, "subject_cov": 0.554945054945055, "identity": 1.0, "score": 205.0, "evalue": 6.07e-66, "gene_start": 1, "gene_end": 101, "reference_start": 1, "reference_end": 101, "reference_sequence": "MNTVRVDIDSILAGIAENPNISDLHLTCSGKSSYRLNGDIIKDEKIPIINSENMEIILKQLFQNNAQNFDKFICDKESDFAYEGKDGTSYRVNAFFEKGKIGIVMRKINSRIRTLEEMMFSNLAESIKRNVLAAKKRTFPSDRIYRKRKINITSKYDGIYQQNKKRKHHHDRRPYRIYLGRR" }, "paralog": false, "identity": 0.967032967032967, "score": 341.0, "evalue": 4.59e-122, "description": "Internal stop codon at 21964, 22132, 22186, 22198. Frameshift due to deletion around 22063. Nonsense mutation around 21964.", "psc": { "uniref90_id": "UniRef90_A0A328ECY0", "product": "Bacterial type II secretion system protein E domain-containing protein", "uniref50_id": "UniRef50_A0A328ECY0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328ECY0", "UniRef:UniRef50_A0A328ECY0" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328ECY0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328ECY0" ], "product": "Bacterial type II secretion system protein E domain-containing protein" } }, "id": "NKOCPLAKPK_1966", "locus": "GCA003260355_01957" }, { "type": "cds", "sequence": "contig_3", "start": 21973, "stop": 22536, "strand": "+", "frame": 1, "gene": null, "product": "PilT/PilU family type 4a pilus ATPase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2G6CBS5", "UniRef:UniRef90_A0A660MSM6" ], "nt": "ATGAGAAAAATCAATTCTCGCATCAGAACGCTTGAAGAGATGATGTTCTCTAATCTCGCCGAAAGTATAAAAAGGAACGTCCTTGCAGCAAAAAAAGGACTTTTCCTAGTGACAGGATCTACAGGAAGCGGAAAATCAACCTCACTAGTAAGTATGATTGAATATATCAACAAAACAAGAAACGAAAACATCATCACTATCGAAGACCCTATTGAATATATCTTTGAAGGAGATAAATGTAGTATTTCACAGAGACAGGTAGGACATGATACCTGGTCATTCAAAAACGCACTAAAAGCTGTAATGAGAGAAGATCCTGATATCATTTTTGTAGGAGAAATTCGTGATACTGAAACTGCAGAAACCGTCCTTAGCCTAGCAGAAAGTGGACATCTCGTTTTTAGCACCTTGCACACAAGCTCAGCAAGTCATAGTTTGAGTAGATTTATGTCCTTTTTCCCTACAAATATGGAACAAAGTATCGCCGACAGACTTTCAGATAGTCTCATCGGAATCCAATCTCAAATGCTCGTAAAAAGAACAGATACCAATACGAGAATCTGA", "aa": "MRKINSRIRTLEEMMFSNLAESIKRNVLAAKKGLFLVTGSTGSGKSTSLVSMIEYINKTRNENIITIEDPIEYIFEGDKCSISQRQVGHDTWSFKNALKAVMREDPDIIFVGEIRDTETAETVLSLAESGHLVFSTLHTSSASHSLSRFMSFFPTNMEQSIADRLSDSLIGIQSQMLVKRTDTNTRI", "aa_hexdigest": "078af2cd24ae6c807f1886b9b4a44d09", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 21673, "stop": 22749, "observations": { "mutation": [ 21964, 22534 ], "stop": [ 21964, 22534 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A660MSM6", "query_cov": 1.0, "subject_cov": 0.5165745856353591, "identity": 1.0, "score": 356.0, "evalue": 1.35e-121, "gene_start": 1, "gene_end": 187, "reference_start": 105, "reference_end": 291, "reference_sequence": "MNTVRVDIDSILAGIAENPNISDLHLTCSGKSSYRLNGDIIKDEKIPIINSENMEIILKQLFQNNAQNFDKFICDKESDFAYEGKDGTSYRVNAFFEKGKIGIVMRKINSRIRTLEEMMFSNLAESIKRNVLAAKKGLFLVTGSTGSGKSTSLVSMIEYINKTRNENIITIEDPIEYIFEGDKCSISQRQVGHDTWSFKNALKAVMREDPDIIFVGEIRDTETAETVLSLAESGHLVFSTLHTSSASHSLSRFMSFFPTNMEQSIADRLSDSLIGIQSQMLVKRTDTNTRIGIFELLLNTVAIKNNLKKMDLGQVDSTIESSNPLGMISMKQYAKKLVDRQLINPKDVKHLFRVEERATETD" }, "paralog": false, "identity": 0.994413407821229, "score": 689.0, "evalue": 4.04e-255, "description": "Internal stop codon at 21964, 22534. Nonsense mutation around 21964, 22534.", "psc": { "uniref90_id": "UniRef90_A0A660MSM6", "product": "PilT/PilU family type 4a pilus ATPase", "uniref50_id": "UniRef50_A0A2G6CBS5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A660MSM6", "UniRef:UniRef50_A0A2G6CBS5" ] }, "pscc": { "uniref50_id": "UniRef50_A0A2G6CBS5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2G6CBS5" ], "product": "Bacterial type II secretion system protein E domain-containing protein" } }, "id": "NKOCPLAKPK_1967", "locus": "GCA003260355_01958" }, { "type": "cds", "sequence": "contig_3", "start": 22588, "stop": 22749, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATCTTGGACAAGTTGATTCGACGATCGAATCTTCAAATCCACTAGGAATGATTTCAATGAAACAATACGCAAAGAAACTCGTAGATAGGCAACTCATCAACCCTAAAGACGTAAAACATCTCTTTAGAGTAGAAGAAAGAGCAACCGAAACAGACTAA", "aa": "MDLGQVDSTIESSNPLGMISMKQYAKKLVDRQLINPKDVKHLFRVEERATETD", "aa_hexdigest": "e6d1784cae78b0150b8e268eb0ca5e11", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6076.8885, "isoelectric_point": 5.697506523132325 }, "id": "NKOCPLAKPK_1968", "locus": "GCA003260355_01959" }, { "type": "cds", "sequence": "contig_3", "start": 22823, "stop": 22978, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EC72", "UniRef:UniRef90_A0A328EC72" ], "nt": "ATGAAAAGACTTTCAAGGGATGAGATCAGAAAAATGAAAGAAAATATGCAAAAAACACCACTCATCCAAAAGCAAGCTGAACAATATCACAATAAAGAAAGTAAAGAAGCTGAAGCATTATTGAACAAGATAGAGATTCCTTCAGAAAAACAATGA", "aa": "MKRLSRDEIRKMKENMQKTPLIQKQAEQYHNKESKEAEALLNKIEIPSEKQ", "aa_hexdigest": "c17ea922c44117ce22b97c3df465439f", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 22793, "stop": 23047, "observations": { "mutation": [ 22811, 22976, 22979, 23012 ], "stop": [ 22811, 22976, 22979, 23012 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EC72", "query_cov": 1.0, "subject_cov": 0.6, "identity": 1.0, "score": 93.6, "evalue": 5.74e-24, "gene_start": 1, "gene_end": 51, "reference_start": 11, "reference_end": 61, "reference_sequence": "MQETYSGKSGMKRLSRDEIRKMKENMQKTPLIQKQAEQYHNKESKEAEALLNKIEIPSEKQGGKLDIQPPKKIGFFQKIKNYLFG" }, "paralog": false, "identity": 0.9523809523809523, "score": 154.0, "evalue": 1.32e-50, "description": "Internal stop codon at 22811, 22976, 22979, 23012. Nonsense mutation around 22811, 22976, 22979, 23012.", "psc": { "uniref90_id": "UniRef90_A0A328EC72", "uniref50_id": "UniRef50_A0A328EC72", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EC72", "UniRef:UniRef50_A0A328EC72" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EC72", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EC72" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 6140.0374999999985, "isoelectric_point": 9.397121238708493 }, "id": "NKOCPLAKPK_1969", "locus": "GCA003260355_01960" }, { "type": "cds", "sequence": "contig_3", "start": 23063, "stop": 23467, "strand": "+", "frame": 2, "gene": null, "product": "Rho termination factor N-terminal domain-containing protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DC050F9", "UniRef:UniRef100_A0A328EBI0", "UniRef:UniRef50_A0A3D0USM5", "UniRef:UniRef90_A0A328EBI0" ], "nt": "ATGAATCCAGTATTACAGCAGTATCTACCACAAGAGCTACGGGAAATCGCAGCAGACTTCAAAATTCCTGAAGCCTTCTTGGTAAATAACTCAAACCTTATTCAGCTTATCCTAAAATCAAAATCTCTCGCCGAATACGAAGAGAAACAGAATTGGTTTAATCTTTTACCAATCATGTCTCCTGAACAAATAGAAAAACTCAGAGATATTCTCACAAGAGAACAACAAAAACTTGAGGAAATCAACCAAAAATACAGCCAAAAACAGGCTGAAATTTCCGAAAAATACCAGCAAAGTTTCAATCCTGCACTCTATAGCCAAGCACAGGCAAAAATCCATGCCCAAGAAAACGAAGCAAGAGAACAAGAAATGATTGAAGCAGACAATTTACTGACCCAAATGTAA", "aa": "MNPVLQQYLPQELREIAADFKIPEAFLVNNSNLIQLILKSKSLAEYEEKQNWFNLLPIMSPEQIEKLRDILTREQQKLEEINQKYSQKQAEISEKYQQSFNPALYSQAQAKIHAQENEAREQEMIEADNLLTQM", "aa_hexdigest": "afab72393a091aba20dbd03a6ef93699", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000DC050F9", "uniref100_id": "UniRef100_A0A328EBI0", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DC050F9", "UniRef:UniRef100_A0A328EBI0" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EBI0", "uniref90_id": "UniRef90_A0A328EBI0" }, "psc": { "uniref90_id": "UniRef90_A0A328EBI0", "uniref50_id": "UniRef50_A0A3D0USM5" }, "pscc": { "uniref50_id": "UniRef50_A0A3D0USM5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3D0USM5" ], "product": "Rho termination factor N-terminal domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_1970", "locus": "GCA003260355_01961" }, { "type": "cds", "sequence": "contig_3", "start": 23361, "stop": 23819, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATCATCGTTACCGCTAGGACCATTGTCATTCTGAGCAAGAACTTTGAAATACTAATAATAAGTGATGGTCTACGATTGATTCCTTTATTTTTTCATCAGATTTTTATTTCTGTTCCTTCTTTTAGGATGTATTTTCCTACGCTCATTTTGTCGGTTCCAATACTATCTCATCCTACGATAGCATCAGTTTGGATCGCTGGTGTAAATGCTTGTTCAAGAAAGTCTCCGTTGGTATTCCCTGCTTGTCCTTTTGCGTATCCGATTCATAGAGAAAAGATTGCTATACTTCCTAAGCTACTAAGCATAAAACTAAAAATTCTGATTTTCTTGGCTATTGATATTTTCATATTTACATTTGGGTCAGTAAATTGTCTGCTTCAATCATTTCTTGTTCTCTTGCTTCGTTTTCTTGGGCATGGATTTTTGCCTGTGCTTGGCTATAGAGTGCAGGATTGA", "aa": "MIIVTARTIVILSKNFEILIISDGLRLIPLFFHQIFISVPSFRMYFPTLILSVPILSHPTIASVWIAGVNACSRKSPLVFPACPFAYPIHREKIAILPKLLSIKLKILIFLAIDIFIFTFGSVNCLLQSFLVLLLRFLGHGFLPVLGYRVQD", "aa_hexdigest": "0cac4afcddb8cb1aa7f826b1bb82c57c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17152.74249999998, "isoelectric_point": 9.950195503234863 }, "id": "NKOCPLAKPK_1971", "locus": "GCA003260355_01962" }, { "type": "cds", "sequence": "contig_3", "start": 24030, "stop": 24182, "strand": "+", "frame": 3, "gene": null, "product": "DUF4134 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328ECV9", "UniRef:UniRef90_A0A328ECV9" ], "nt": "ATGGCAAAAAAAATATTCTCCACCGTCATCCTCGCATTGTTTGCCACGCTCCCTGTCTTCGGGATCAAAGTTACCGTTCCACAAGGACACGCAGGAGATGATCTTCCAAAAAAACACGTCGTCGATACGCAAGAGGAAGGAATATTTCAGTGA", "aa": "MAKKIFSTVILALFATLPVFGIKVTVPQGHAGDDLPKKHVVDTQEEGIFQ", "aa_hexdigest": "fff0cc12fc3ec7b01b2a04c19da866a2", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 24030, "stop": 24386, "observations": { "mutation": [ 24180, 24324 ], "stop": [ 24180, 24324 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328ECV9", "query_cov": 1.0, "subject_cov": 0.423728813559322, "identity": 1.0, "score": 101.0, "evalue": 1.25e-26, "gene_start": 1, "gene_end": 50, "reference_start": 1, "reference_end": 50, "reference_sequence": "MAKKIFSTVILALFATLPVFGIKVTVPQGHAGDDLPKKHVVDTQEEGIFQGINIINKYLRWSFAVVCTAVVVYGGYQLITANGDKKAMKKAVWALMGAGIGITIAMLSYTIVSLLTNL" }, "paralog": false, "identity": 0.9830508474576272, "score": 227.0, "evalue": 1.15e-78, "description": "Internal stop codon at 24180, 24324. Nonsense mutation around 24180, 24324.", "psc": { "uniref90_id": "UniRef90_A0A328ECV9", "product": "DUF4134 domain-containing protein", "uniref50_id": "UniRef50_A0A328ECV9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328ECV9", "UniRef:UniRef50_A0A328ECV9" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328ECV9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328ECV9" ], "product": "DUF4134 domain-containing protein" } }, "id": "NKOCPLAKPK_1972", "locus": "GCA003260355_01963" }, { "type": "cds", "sequence": "contig_3", "start": 24696, "stop": 24830, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAACTTTCTTATATCTCTATGCCAAACTAAGCAAACTGATTCCTAAATTCTACAGGAACATCCTCGTTTACGATTCCAGAGATATAGTGGTTAAAATCTCACATAGTGATATAATTTTTTCCTCTAGCTAA", "aa": "MATFLYLYAKLSKLIPKFYRNILVYDSRDIVVKISHSDIIFSSS", "aa_hexdigest": "2bcb02b6bfaab65d620b18dbb16f57a9", "start_type": "ATG", "rbs_motif": "TTTTTA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5157.0328, "isoelectric_point": 9.52193202972412 }, "id": "NKOCPLAKPK_1973", "locus": "GCA003260355_01964" }, { "type": "cds", "sequence": "contig_3", "start": 24798, "stop": 25085, "strand": "-", "frame": 2, "gene": null, "product": "Transglycosylase SLT domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EEZ0", "UniRef:UniRef90_A0A328EEZ0" ], "nt": "TTGATCCAATTTATTCCGAGTACTGCTAGAAGGGTCGGAACAACGGTCTATGCTCTTAGATCTATGTCTGCTCTTCAACAGCTTGATTATGTGAAAAAGTATTATCAACTTTCTCCAGGGCAAAAGTTTAGGTCTCTCAAAGATCTTTATCTCTATACTTTTTTCCCGATAGCTATGAATCATTCAAGTAATCCTAATTATGTTTTTAAGTCTAATAAAACTTCTGCCGCAGAACTTGCAGCGTTGCATAGAAAGTTAGCTAGAGGAAAAAATTATATCACTATGTGA", "aa": "MIQFIPSTARRVGTTVYALRSMSALQQLDYVKKYYQLSPGQKFRSLKDLYLYTFFPIAMNHSSNPNYVFKSNKTSAAELAALHRKLARGKNYITM", "aa_hexdigest": "dcbc7b10f5206a6b12ab11214f124e57", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 24726, "stop": 25205, "observations": { "mutation": [ 24800, 25097 ], "stop": [ 24800, 25097 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EEZ0", "query_cov": 1.0, "subject_cov": 0.5974842767295597, "identity": 0.9890000000000001, "score": 188.0, "evalue": 7.96e-60, "gene_start": 1, "gene_end": 95, "reference_start": 41, "reference_end": 135, "reference_sequence": "MEKIAAELDINPNRLMALMASETGGTFNPAIVNKSTGATGLIQFIPSTARRVGTTVYALRSMSALQQLDYVKKYYQLSPGQKFRSLKDLYLYTFFPIAMNHSSNPNYVFKSNKTSAAELAALHRKLARGKNYITMGDFNHYISGIVNEDVPVEFRNQFA" }, "paralog": false, "identity": 0.9874213836477987, "score": 309.0, "evalue": 6.94e-110, "description": "Internal stop codon at 24800, 25097. Nonsense mutation around 24800, 25097.", "psc": { "uniref90_id": "UniRef90_A0A328EEZ0", "product": "Transglycosylase SLT domain-containing protein", "uniref50_id": "UniRef50_A0A328EEZ0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EEZ0", "UniRef:UniRef50_A0A328EEZ0" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EEZ0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EEZ0" ], "product": "Transglycosylase SLT domain-containing protein" } }, "id": "NKOCPLAKPK_1974", "locus": "GCA003260355_01965" }, { "type": "cds", "sequence": "contig_3", "start": 25095, "stop": 25205, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAGAAAATTGCAGCAGAGCTTGATATTAATCCTAATCGATTGATGGCGCTGATGGCGTCAGAAACAGGGGGAACATTTAATCCAGCTATAGTAAATAAATCAACATGA", "aa": "MEKIAAELDINPNRLMALMASETGGTFNPAIVNKST", "aa_hexdigest": "327e3416af1b580474b4027ca7293f8e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3849.4126000000006, "isoelectric_point": 4.867827796936035 }, "id": "NKOCPLAKPK_1975", "locus": "GCA003260355_01966" }, { "type": "cds", "sequence": "contig_3", "start": 25239, "stop": 26267, "strand": "-", "frame": 2, "gene": null, "product": "Transglycosylase SLT domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MUP1", "UniRef:UniRef90_A0A660MUP1" ], "nt": "TTGGAAAAGGCGGACGGTGTTTTTCAGAATATTGATGCTCTAGGAAATAAACTACTTGATAGTATGGATGTTTTGGGAAATACTTCAAAAGTTTTGAAATCAACGCTAGGATTTGATCTTTGGAAGCAAATCAGGAAAACTCCAGCACTTGGAGGAGTTCTTAATGTTGTTTTAGGTTTGCTTGGTTTCTCTGGGGGAGTTCAAGGATTGGAAAGAGCTTGGAAAAAAAGGATTCTTGAGAGGGAGCTTAAACAGCCTCAGAAAGCATATATTGCTGAGTCTTATAAAAATTATATGCAGAAGAAAGCTCTTCAAGCTACTACCGCAAAAACACTTCTTGATCAACATAATCTCAAAGTGGAGGATGCTGCTCAGCAGAAGTTTGCTCTGGATATGCCTCTGATAAAAAGTCAGATTCTTCAAAAAATAGATGAAAAACCTGAACTTTTAAATCTTTCTACTTTGAAAATGACTAAAAGTCCTGTTTTTAAGGGAGAAGATTTTGTGCAAGAAGTAAAGGTTGGAGAAAAAACGAAGCTTACTCTCAAAAGAAAACTCTCTCCACAGGAAAGAGAAGCTTTTGTGGATACTTATCTCCATGAGGGATTGGAGCGTTTTGCTTCAAATCCGAAAGCTTTATCTTCACTACCTAATGCTGATACTTTAGCGTTTATTCTCCTCTCAGGGGTTGCTCTTAATAAAGATGATGTGATAGATGGTGTGGAGGCTCAGGTTTTTCTACCTGATCAATATACTGATGCAGCACTAGCAACTCAAAATGGGCAGGATAGATATATTTATCCTAATGGATCTACTCCTATTGGAAATAATAAGCCTGTAGTGAATCCTAATAACCCTGTAGTGAATCCTATTTCTCCAAATCCTTCTCCAGAAAATATCAATAATTTTGTGGAGAATATTGGTCAAAAACCAGAATCTGGCAATGATATAAACAATAATCAGTTAGATGAAGCTTTGTCAAGACATGGATATAATGGAAGGCTTCTTTTGTATGATAAGGTTCCTTGA", "aa": "MEKADGVFQNIDALGNKLLDSMDVLGNTSKVLKSTLGFDLWKQIRKTPALGGVLNVVLGLLGFSGGVQGLERAWKKRILERELKQPQKAYIAESYKNYMQKKALQATTAKTLLDQHNLKVEDAAQQKFALDMPLIKSQILQKIDEKPELLNLSTLKMTKSPVFKGEDFVQEVKVGEKTKLTLKRKLSPQEREAFVDTYLHEGLERFASNPKALSSLPNADTLAFILLSGVALNKDDVIDGVEAQVFLPDQYTDAALATQNGQDRYIYPNGSTPIGNNKPVVNPNNPVVNPISPNPSPENINNFVENIGQKPESGNDINNNQLDEALSRHGYNGRLLLYDKVP", "aa_hexdigest": "514dfb957def0862aea3ed05dd1fef1a", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 24936, "stop": 26567, "observations": { "mutation": [ 25097, 25241, 26273, 26321 ], "stop": [ 25097, 25241, 26273, 26321 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A660MUP1", "query_cov": 1.0, "subject_cov": 0.41007194244604317, "identity": 0.971, "score": 591.0, "evalue": 1.01e-204, "gene_start": 1, "gene_end": 342, "reference_start": 322, "reference_end": 663, "reference_sequence": "MNYRFIDFPTDWSQPKNSKDDVKVPEKILSSQQKTKEKIQTFPDKKLEPVEKKDSSLDFVKEGIGNVKTSIENLNYGMNELVTLDTLDGTPLKNTMLSILKNPQFDDYPDLKGKTPEQRLEFVFRKINIALTKFYARKLNIGLTEPVPDYLKKVVIPATELFLMETLAQMGNETNINFLGQLSSMDFSSLGNLVTQVKDFSEKFSFSFGQGKTLLNLTDFLALPKNQAQLSKLDDPYEFYQKVLKNPLWKQDITTKDSPKPNQISLDSITWEQFDLRALKNNLSNEEKQQAFQMGKEKMKQQLGSIKMVETPETMKKMLGILEKADGVFQNIDALGNKLLDSMDVLGNTSKVLKSTLGFDLWKQIRKTPALGGALNVVLGLLGFSGGVEGLERAWKKRILERELKQPQKAYIAESYKNYMQKKALQATTAKTLLDQHNLKVEDAAQQKFALDMPLIKSQILQKIGEKPELLNLSTLKMTKSPVFKGEDFVQEVKVGDKTKLTLKRKLSPQEREAFVDTYLHEGLERFASNPKALSSLPNADTLAFILLSGVALNKDDVIDGVEAQVFLPDQYTDAALATQNGQDRYIYPNGSTPIGNNKPVVNPNNPVVNPISPNPSAENINNFVENIGQKPESGNDINNDQLDEALGRHGYNGSLLFYDKVPGTEEQKKAFKQKMEKIAAELDINPNRLMALMASETGGTFNPAIANKSTGATGLIQIMPSTATKLGTTVSALRSMSAVQQLDYVKKYYQLSPGKKFRSLKDLYLYTFFPIAMNHSSNPNYVFQSSTISAAKLAGLHRKLARGKSYITMGDFNHYISGMVNQNVPVEFRNQFA" }, "paralog": false, "identity": 0.9594843462246777, "score": 966.0, "evalue": 0.0, "description": "Internal stop codon at 25097, 25241, 26273, 26321. Nonsense mutation around 25097, 25241, 26273, 26321.", "psc": { "uniref90_id": "UniRef90_A0A660MUP1", "product": "Transglycosylase SLT domain-containing protein", "uniref50_id": "UniRef50_A0A660MUP1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A660MUP1", "UniRef:UniRef50_A0A660MUP1" ] }, "pscc": { "uniref50_id": "UniRef50_A0A660MUP1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MUP1" ], "product": "Transglycosylase SLT domain-containing protein" } }, "id": "NKOCPLAKPK_1976", "locus": "GCA003260355_01967" }, { "type": "cds", "sequence": "contig_3", "start": 26319, "stop": 26999, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGAGCTTGTGACTTTGGATACTCTTGATGGAACTCCGCTCAAAAATACCATGCTTTCTATATTAAAGAATCCTCAATTTGATGATTATCCTGATTTGAAAGGAAAAACTCCAGAACAAAGGTTAGAGTTTGTTTTTAGGAAGATAAATATTGCTTTAACAAAGTTTTATGCTAGAAAACTAAATATTGGGCTGACTGAACCAGTTCCAGATTATCTCAAGAAGGTAGTTATTCCTGCTACGGAATTGTTTTTGATGGAGACTTTGGCTCAAATGGGAAATGAGACGAATATCAATTTTTTGGGACAGCTATCTAGTATGGATTTTAGTAGTTTAGGGAATCTTGTAAACCAGGTAAAAGATTTTTCTGAGAAGTTTTCGTTTTCTTTTGGTCAAGGAAAGACACTTTTGAATCTTACAGACTTTCTTGCTTTACCAAAAAATCAAGCTCAATTAAGTAAGTTGGATGATCCTTATGAATTTTATCAAAAAGTGCTCAAAAATCCTCTCTGGAAGCAAGACATTACTACAAAGGATAGTCCAAAACCGAATCAGATTTCACTTGATTCAATTACTTGGGAACAGTTTGATTTGCGTGCTCTTAAAAATAACCTTTCTAATGAAGAGAAACAGCAGGCTTTCCAAATGGGAAAGGAAAAAATGAAACAACAGCTTTGA", "aa": "MNELVTLDTLDGTPLKNTMLSILKNPQFDDYPDLKGKTPEQRLEFVFRKINIALTKFYARKLNIGLTEPVPDYLKKVVIPATELFLMETLAQMGNETNINFLGQLSSMDFSSLGNLVNQVKDFSEKFSFSFGQGKTLLNLTDFLALPKNQAQLSKLDDPYEFYQKVLKNPLWKQDITTKDSPKPNQISLDSITWEQFDLRALKNNLSNEEKQQAFQMGKEKMKQQL", "aa_hexdigest": "369483728d800da7f446aecc79e10c13", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 25997.589600000003, "isoelectric_point": 6.404924583435059 }, "id": "NKOCPLAKPK_1977", "locus": "GCA003260355_01968" }, { "type": "cds", "sequence": "contig_3", "start": 27042, "stop": 27230, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATTATAGATTTATTGATTTCCCTACTGATTGGTCTCAACCTAAAAATTCTAAAGATGGTGTGAAGGTGCCCGAGAAGATTCTATCTTCACAACAAAAAACTAAAGAGAAGATTCAGACTTTCCCTGATAAAAAACTAGAACCAGTGGAGAAAAAGGACTCTTCATTAGATTTTGTTAAAGAATGA", "aa": "MNYRFIDFPTDWSQPKNSKDGVKVPEKILSSQQKTKEKIQTFPDKKLEPVEKKDSSLDFVKE", "aa_hexdigest": "50ea7135e3cc22ea5acb54dda56ead7d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7302.231700000003, "isoelectric_point": 8.926050758361818 }, "id": "NKOCPLAKPK_1978", "locus": "GCA003260355_01969" }, { "type": "cds", "sequence": "contig_3", "start": 27382, "stop": 27495, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAATCCTTGCATTTCTGCTGATCACTTCGCTTTTTTTTCAGCCACTTGCAATTTTTGCAGAAGAAATAGAATCTCATCCAGAACCAACCATTTCAAGTGAAGTTTGA", "aa": "MKKILAFLLITSLFFQPLAIFAEEIESHPEPTISSEV", "aa_hexdigest": "f42f3c16d140d82cfdadbe8a28d70131", "start_type": "ATG", "rbs_motif": "TTATT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4190.8929, "isoelectric_point": 4.636265754699706 }, "id": "NKOCPLAKPK_1979", "locus": "GCA003260355_01970" }, { "type": "cds", "sequence": "contig_3", "start": 27462, "stop": 27632, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTTCACTAGAGCTATTCCCTATGTTTTCTCCAGTTATTGAATTGTTTGTTTCTGCAATGTTTCAAGAAAAGGATTCTTCTGATTTTTTTTGTGATGACTGGATTTCTGGCTTATTTTCTTCTGCTCAGGGTTGTCAAACTTCACTTGAAATGGTTGGTTCTGGATGA", "aa": "MFSLELFPMFSPVIELFVSAMFQEKDSSDFFCDDWISGLFSSAQGCQTSLEMVGSG", "aa_hexdigest": "75e7b4a97016f16afe658d388487bb13", "start_type": "ATG", "rbs_motif": "TTTTT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6228.015399999998, "isoelectric_point": 4.0500284194946286 }, "id": "NKOCPLAKPK_1980", "locus": "GCA003260355_01971" }, { "type": "cds", "sequence": "contig_3", "start": 28537, "stop": 28695, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAACAGGAACGATTTTAGCCAAAGAAATAGTCTTAGAAGAAACAGATTTTCGAGAAAAAAACAGCACCTTCATCATCAGTAAAGATGAAACACGATACGACAAAGGAATCGACAGTCAATCTAATCATGACTTTAATCTACTATATCAGACTTGA", "aa": "MLTGTILAKEIVLEETDFREKNSTFIISKDETRYDKGIDSQSNHDFNLLYQT", "aa_hexdigest": "da3dd4a27e445d35fc39e0c96f966f28", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6069.672300000002, "isoelectric_point": 4.642290687561036 }, "id": "NKOCPLAKPK_1981", "locus": "GCA003260355_01972" }, { "type": "cds", "sequence": "contig_3", "start": 29611, "stop": 29955, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00565.23" ], "nt": "TTGGCATACGTATCACTAGACGGGAAGCTCATTAATGAAGAACTTATCCGCAATGGATACGCAAAAGAATACACCTACAAACAAAGTTATGAAAAACAAGAAGCTTTCAAAAAAGCAGAATATGAAGCAAAAAACGAACAACTAGGAATACGAGATCCAAAAAACTGTCCAGATCCTTCGCTAAAAGATGAAGAAAAGAAAAACAATATTGAAAATCTAATCATAAAAATCCAAAACATTGACTATAACCCCAAAGGAAGTGATAAAAATAATGAAAGTATCACACTATCTATCTTTGATAAATCAAAAATGATAAAATCTTTAGATTTTAATAATCAGTTTTGA", "aa": "MAYVSLDGKLINEELIRNGYAKEYTYKQSYEKQEAFKKAEYEAKNEQLGIRDPKNCPDPSLKDEEKKNNIENLIIKIQNIDYNPKGSDKNNESITLSIFDKSKMIKSLDFNNQF", "aa_hexdigest": "d1682010af2196a8cb8ca439eb4819bf", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00565.23", "name": "SNase", "length": 52, "aa_cov": 0.43859649122807015, "hmm_cov": 1.0, "evalue": 2.3068427321496106e-09, "score": 34.15121841430664, "start": 1, "stop": 50 } ], "seq_stats": { "molecular_weight": 13319.812399999983, "isoelectric_point": 5.5921838760375975 }, "id": "NKOCPLAKPK_1982", "locus": "GCA003260355_01973" }, { "type": "cds", "sequence": "contig_3", "start": 30760, "stop": 31047, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTCAAAACAGAATATTCTCCACGATACCAACAAACAAAAGTAAGTGAATACTTTTCCCTCGCAAAAAAGATGAAGTCAGATCTAAAAAATAATATTTTCTCAACCAACATAGACGAGGATAGCTATGAATGGAAAGCTTTTCCAAAAATCTTTGAAAAAGAAACAGATCAAAGCCTGATCGATACCTCCGAAAAAATCTTAAAAAAAATCTTCTCCTCAGAAATAAGATCACGCTATGAAGAGAAGAAACAACAACGAATAGCAGCACTTCCAGACACAATCTAA", "aa": "MLKTEYSPRYQQTKVSEYFSLAKKMKSDLKNNIFSTNIDEDSYEWKAFPKIFEKETDQSLIDTSEKILKKIFSSEIRSRYEEKKQQRIAALPDTI", "aa_hexdigest": "a950c55b05ff80e80671d60aa157897b", "start_type": "ATG", "rbs_motif": "TTTTT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11338.751299999987, "isoelectric_point": 8.751921653747559 }, "id": "NKOCPLAKPK_1983", "locus": "GCA003260355_01974" }, { "type": "cds", "sequence": "contig_3", "start": 31054, "stop": 31218, "strand": "-", "frame": 1, "gene": null, "product": "SHSP domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A288D9U8", "UniRef:UniRef90_A0A328ECX6" ], "nt": "GTGAAAGAAGAATGTTATCGAGGAAAAATTAGACAAATCATCGATCTTCCTGCTCAGATTTATTTTGATAGAATTCATAGTAAACTTTCTCCGGAAAATATTTTGTGGATTATTATTCCTAAAGCTCTTGTTCCTGAAAAGATAACGCTCCAGATTGAAGAATAG", "aa": "MKEECYRGKIRQIIDLPAQIYFDRIHSKLSPENILWIIIPKALVPEKITLQIEE", "aa_hexdigest": "6cc3d3f95a9a4c9fccd0eb27c1a22ff1", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 31054, "stop": 31398, "observations": { "mutation": [ 31320 ], "stop": [ 31320 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328ECX6", "query_cov": 1.0, "subject_cov": 0.47368421052631576, "identity": 0.981, "score": 111.0, "evalue": 1.46e-30, "gene_start": 1, "gene_end": 54, "reference_start": 61, "reference_end": 114, "reference_sequence": "MQLDNSPLIPYDIYESSQEIVIIMPLGGVKKETIRLTIEDYKLILEGERILTPLKENLLPVKEECYRGKIRQIIDLPAQIYFDRIHSKLSPENILWIIIPKALVPEKITLQIEE" }, "paralog": false, "identity": 0.9912280701754386, "score": 223.0, "evalue": 3.83e-77, "description": "Internal stop codon at 31320. Nonsense mutation around 31320.", "psc": { "uniref90_id": "UniRef90_A0A328ECX6", "product": "SHSP domain-containing protein", "uniref50_id": "UniRef50_A0A288D9U8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328ECX6", "UniRef:UniRef50_A0A288D9U8" ] }, "pscc": { "uniref50_id": "UniRef50_A0A288D9U8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A288D9U8" ], "product": "SHSP domain-containing protein" } }, "id": "NKOCPLAKPK_1984", "locus": "GCA003260355_01975" }, { "type": "cds", "sequence": "contig_3", "start": 31630, "stop": 31746, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCTGTTTATCATTTATCTTTTTGTTATTGCTCCTTTGTTTAAGTATTATTTGGACTATGTTATGGATTTTTCTCTCGTTACTCCCGAGTATCTTACGAGATACAATCAGACTTGA", "aa": "MLFIIYLFVIAPLFKYYLDYVMDFSLVTPEYLTRYNQT", "aa_hexdigest": "97e535eb474ced2a94f4bdb6e57762fd", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4705.529000000001, "isoelectric_point": 4.556577491760254 }, "id": "NKOCPLAKPK_1985", "locus": "GCA003260355_01976" }, { "type": "cds", "sequence": "contig_3", "start": 32655, "stop": 32756, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATTATTGATAATTTTATTTTCCTTGATACGAAAGTTTCGAACTCAGATGAAGTAGAGATTGATCCGCTCTCTGATTTTGATAGTGAGGTTCCTTTTTAA", "aa": "MIIDNFIFLDTKVSNSDEVEIDPLSDFDSEVPF", "aa_hexdigest": "ef8ecd9f0775d0e77d07f68afb596234", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3792.1324999999997, "isoelectric_point": 4.0500284194946286 }, "id": "NKOCPLAKPK_1986", "locus": "GCA003260355_01977" }, { "type": "cds", "sequence": "contig_3", "start": 32835, "stop": 33050, "strand": "-", "frame": 2, "gene": null, "product": "Single-stranded DNA-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1V5ZP08" ], "nt": "ATGGATTTGAATAAGGTAATGTTGATTGGGAGGTCTACGAGTACTTTACAGGTCAAGACTATAGAGAGCACTCAAAGTGCTGTCGTTAATTTCACTCTTGCAACCAATAGAAAGTATAAAAATAAAGAAGGAAATGTTTTGGAAGATGCTGAATATCATAGATGTGTTGCCTATGGGAATACCGCAGAAGTGCTTGGAAAGTATCTTACTAAGTGA", "aa": "MDLNKVMLIGRSTSTLQVKTIESTQSAVVNFTLATNRKYKNKEGNVLEDAEYHRCVAYGNTAEVLGKYLTK", "aa_hexdigest": "887b42169ba29fcf944e33b2a5b9d39a", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A1V5ZP08", "query_cov": 0.8309859154929577, "subject_cov": 0.9833333333333333, "identity": 0.746, "score": 93.6, "evalue": 6.8e-24, "valid": false, "product": "Single-stranded DNA-binding protein", "uniref50_id": "UniRef50_A0A1V5ZP08" }, "pscc": { "uniref50_id": "UniRef50_A0A1V5ZP08", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1V5ZP08" ], "product": "Single-stranded DNA-binding protein" }, "genes": [], "id": "NKOCPLAKPK_1987", "locus": "GCA003260355_01978" }, { "type": "tRNA", "sequence": "contig_3", "start": 33254, "stop": 33337, "strand": "-", "gene": "trnL", "product": "tRNA-Leu(caa)", "amino_acid": "Leu", "anti_codon": "caa", "score": 58.2, "nt": "GCCTAGGTGATGGAACTGGTAGACATGCTAGACTCAAAATCTGGTGGCTTCACGGCCGTGCGGGTTCAAGTCCCGCTCTAGGTA", "db_xrefs": [ "SO:0000264" ], "anti_codon_pos": [ 33301, 33303 ], "id": "NKOCPLAKPK_41", "locus": "GCA003260355_01979" }, { "type": "tRNA", "sequence": "contig_3", "start": 33391, "stop": 33467, "strand": "-", "gene": "trnG", "product": "tRNA-Gly(gcc)", "amino_acid": "Gly", "anti_codon": "gcc", "score": 77.0, "nt": "GCGGGTGTAGCTCATTTGGCTAGAGCGCTTCCTTGCCAAGGAAGAGGCAGCGGGTTCGAGTCCCGTCACTCGCTCCA", "db_xrefs": [ "SO:0000260" ], "anti_codon_pos": [ 33431, 33433 ], "id": "NKOCPLAKPK_40", "locus": "GCA003260355_01980" }, { "type": "cds", "sequence": "contig_3", "start": 33667, "stop": 33837, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4IGT3", "UniRef:UniRef90_A0A328EC63" ], "nt": "ATGCAGAAACCCATCGTCCTCCCAGAATATCAACATATGAATTTTGTTTTTTGTGTTGTGCTGATTGTTATTTCTCTTTATATCTCGATTTTTTACGGGATTTATCCTATTCATGTGAGGTTTAGTAGAGCTACGCTTTTTGTCTTGGGAATCTCTGCTATTGTGATGTGA", "aa": "MQKPIVLPEYQHMNFVFCVVLIVISLYISIFYGIYPIHVRFSRATLFVLGISAIVM", "aa_hexdigest": "e7998fe876a7de6ddbe045ddc3996e48", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 33505, "stop": 33915, "observations": { "mutation": [ 33582, 33669, 33861 ], "stop": [ 33582, 33669, 33861 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EC63", "query_cov": 1.0, "subject_cov": 0.4117647058823529, "identity": 1.0, "score": 106.0, "evalue": 2.83e-28, "gene_start": 1, "gene_end": 56, "reference_start": 27, "reference_end": 82, "reference_sequence": "MRDALKRIGVITAFGTFSGYLIYSLIMQKPIVLPEYQHMNFVFCVVLIVISLYISIFYGIYPIHVRFSRATLFVLGISAIVMGKTVFLNDPMNDAYFGDLSCVVGVLLLIIGPTNFIMTSGVKKKREEKNLEIIEV" }, "paralog": false, "identity": 0.9705882352941176, "score": 256.0, "evalue": 1.03e-89, "description": "Internal stop codon at 33582, 33669, 33861. Nonsense mutation around 33582, 33669, 33861.", "psc": { "uniref90_id": "UniRef90_A0A328EC63", "uniref50_id": "UniRef50_A0A2W4IGT3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EC63", "UniRef:UniRef50_A0A2W4IGT3" ] }, "pscc": { "uniref50_id": "UniRef50_A0A2W4IGT3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4IGT3" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 6491.876799999998, "isoelectric_point": 9.034808921813966 }, "id": "NKOCPLAKPK_1988", "locus": "GCA003260355_01981" }, { "type": "cds", "sequence": "contig_3", "start": 34277, "stop": 34822, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EFJ8", "UniRef:UniRef90_A0A328EFJ8" ], "nt": "GTGCCCTCCTATACGACGATTCTTTTGAAGTCTCCTATGTCTGAAAAGGTGATTTCTGCAAGCACAAAAAGTCAGACCTTCTTATTAGACAAAAATACTGATTATCTTCTTCAATTTGCCTCTCAGCAGGAGGAGGAGCATAATTTTATCGTGCTTGAAGATCAAGAAGGGAATCTCATCAAAGTAGGACCACAATCTCAGCTCAGGTTTTCTACTCGTGCTTCGAATTTTTCCCTCCAAGAGCAAAAAGGAAGGGTAGCATTTTTTGGGACAGGAGAGTTTTTGCCTCCCGAGCTAATGGAATTTAGGGATCGTTATCAAGCACGTCAAAAGGCGGCCGCCCTTGCCTTGCTCCCGGCTGGACTCAAAAGCAATCCTACATTTCAAAGATGGAGTATCAGGTTTACCTCCTCTTTGGCGAAAGCATTTCCGTTTTGGTATGGAGAAAATGCGAAGTATATTCAGAGCTATCTGCCGTATCTGGGATTAGCAGCGACTCAGAAGCAGCATGGGAAAGTAGACTATTCAATTCTTAAATCCACCTGA", "aa": "MPSYTTILLKSPMSEKVISASTKSQTFLLDKNTDYLLQFASQQEEEHNFIVLEDQEGNLIKVGPQSQLRFSTRASNFSLQEQKGRVAFFGTGEFLPPELMEFRDRYQARQKAAALALLPAGLKSNPTFQRWSIRFTSSLAKAFPFWYGENAKYIQSYLPYLGLAATQKQHGKVDYSILKST", "aa_hexdigest": "7b48f2b4b171a2368247ada6c09cfc3d", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 34211, "stop": 35122, "observations": { "mutation": [ 34270, 34279, 34858 ], "stop": [ 34270, 34279, 34858 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EFJ8", "query_cov": 1.0, "subject_cov": 0.49453551912568305, "identity": 0.9940000000000001, "score": 352.0, "evalue": 8.07e-120, "gene_start": 1, "gene_end": 181, "reference_start": 164, "reference_end": 344, "reference_sequence": "MKPFLISIASLCGIFLFLVVSNGLGFGMEALFGEHLVVWILFGIFWLLGGALWETTDQNTKFFKKLLIIAEVYLFLLIFVQPGINLSWLEFVVLYAAGYGIVHEVMTFRAQKWQTVFFSSCYGVIVALMVSIAALMWWRQPFDEQQFIMQQGSFLFTVFDGRLVPSYTTILLKSPMSEKVISASTKSQTFLLDKNTDYLLQFASQQEEEHNFIVLEDQEGNLIKVGPQSQLRFSTRASNFSLQEQKGRVAFFGTGEFLPPELMEFRDRYQARQKAAALALLPAGLKSNPTFQRWSIRFTSSLAKAFPFWYGENAKYIQSYLPYLGLAATQKQHGKVDYSILKSTGKLGTEKANHKEKYREYFKKLF" }, "paralog": false, "identity": 0.9900990099009901, "score": 594.0, "evalue": 7.71e-218, "description": "Internal stop codon at 34270, 34279, 34858. Nonsense mutation around 34270, 34279, 34858.", "psc": { "uniref90_id": "UniRef90_A0A328EFJ8", "uniref50_id": "UniRef50_A0A328EFJ8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EFJ8", "UniRef:UniRef50_A0A328EFJ8" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EFJ8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EFJ8" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 20592.18589999999, "isoelectric_point": 9.369786643981932 }, "id": "NKOCPLAKPK_1989", "locus": "GCA003260355_01982" }, { "type": "cds", "sequence": "contig_3", "start": 34856, "stop": 35323, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EFJ8", "UniRef:UniRef90_A0A328EFJ8" ], "nt": "ATGGCGTATCGTATGAAACCTTTTCTGATAAGTATTGCAAGTCTGTGCGGGATCTTTCTTTTTTTGGTGGTAAGCAATGGGCTAGGGTTTGGGATGGAAGCGTTGTTTGGAGAGCATCTGGTGGTATGGATTTTGTTTGGGATTTTTTGGTTGCTCGGTGGAGCTCTTTGGGAAACAACGGACCAGAATACGAAGTTCTTTAAAAAACTTCTTATTATCGCAGAAGTCTATCTTTTTTTATTGATTTTCGTCCAGCCGGGGATCAATCTTTCATGGTTAGAGTTTGTGGTGCTGTATGCGGCAGGGTATGGGATTGTACATGAAGTAATGACCTTTAGAGCACAAAAGTGGCAGACGGTTTTCTTTTCTAGCTGTTATGGGGTGATCGTCGCGTTGATGGTCAGCATTGCAGCGTTGATGTGGTGGAGGCAGCCCTTTGATGAGCAGCAATTTATCATGCAGCAATGA", "aa": "MAYRMKPFLISIASLCGIFLFLVVSNGLGFGMEALFGEHLVVWILFGIFWLLGGALWETTDQNTKFFKKLLIIAEVYLFLLIFVQPGINLSWLEFVVLYAAGYGIVHEVMTFRAQKWQTVFFSSCYGVIVALMVSIAALMWWRQPFDEQQFIMQQ", "aa_hexdigest": "6a315bbed8a9ec7ca3a1fe88ced496a0", "start_type": "ATG", "rbs_motif": "TTTA", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 34553, "stop": 35311, "observations": { "mutation": [ 34858 ], "stop": [ 34858 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EFJ8", "query_cov": 0.9741935483870968, "subject_cov": 0.412568306010929, "identity": 1.0, "score": 300.0, "evalue": 4.33e-100, "gene_start": 5, "gene_end": 155, "reference_start": 1, "reference_end": 151, "reference_sequence": "MKPFLISIASLCGIFLFLVVSNGLGFGMEALFGEHLVVWILFGIFWLLGGALWETTDQNTKFFKKLLIIAEVYLFLLIFVQPGINLSWLEFVVLYAAGYGIVHEVMTFRAQKWQTVFFSSCYGVIVALMVSIAALMWWRQPFDEQQFIMQQGSFLFTVFDGRLVPSYTTILLKSPMSEKVISASTKSQTFLLDKNTDYLLQFASQQEEEHNFIVLEDQEGNLIKVGPQSQLRFSTRASNFSLQEQKGRVAFFGTGEFLPPELMEFRDRYQARQKAAALALLPAGLKSNPTFQRWSIRFTSSLAKAFPFWYGENAKYIQSYLPYLGLAATQKQHGKVDYSILKSTGKLGTEKANHKEKYREYFKKLF" }, "paralog": false, "identity": 0.996031746031746, "score": 500.0, "evalue": 5.39e-181, "description": "Internal stop codon at 34858. Nonsense mutation around 34858.", "psc": { "uniref90_id": "UniRef90_A0A328EFJ8", "uniref50_id": "UniRef50_A0A328EFJ8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EFJ8", "UniRef:UniRef50_A0A328EFJ8" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EFJ8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EFJ8" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 17885.17469999998, "isoelectric_point": 6.055478477478027 }, "id": "NKOCPLAKPK_1990", "locus": "GCA003260355_01983" }, { "type": "cds", "sequence": "contig_3", "start": 35783, "stop": 36031, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCAAGCACAGACAGGAGATGATACTCCGATTGTTGTGATTACTGAAGCGAATAGCGGGGCAATTGTAACACAGACGGGAGATTCTCTCATCTATAGAAATGAAGTCTATGGATTTCAAGTAAAACTTGGAGAGGAGACTAAGGGATGTAAGCTCTTTGTGGATATAAATAGCGATAATATGCCTGATAATACTACTTTTCTTCTCTATGGAAAAGTATGGACAGGAGAAGAGAGTCCTCTTGCTTGA", "aa": "MQAQTGDDTPIVVITEANSGAIVTQTGDSLIYRNEVYGFQVKLGEETKGCKLFVDINSDNMPDNTTFLLYGKVWTGEESPLA", "aa_hexdigest": "6c2a16e9e01566684b1ce30e457b79c1", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8946.876699999999, "isoelectric_point": 4.1284093856811515 }, "id": "NKOCPLAKPK_1991", "locus": "GCA003260355_01984" }, { "type": "cds", "sequence": "contig_3", "start": 36416, "stop": 36751, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAGAGATATAGGATTCAGCACAGGATTTTCTCTTTATGATTTTTCTTCATCTACAGGATTACGAATTAATTATCGTTTCTATAAGGAGAAAGATGAAAGATTTTATCCTGATAATCAGCTTTTTTGGCCACTAGTTTTGGAATTAGAAAGGAGCTCTTGTCTCAGAGTTGACTTTTCTTATTTAGTAGCTCCGAGTAAGTGCTTAAATTTAGTTATCAATCCGGTGAGAATTTCAGAACCGCATGCTCTTGTGATCGTGAGAAATACTTCAGAACAGTCTATTTCTTTTTCTTACCTAAAAGGTTATCTTCAGGAAGAGTATCAGAAAGAGTAA", "aa": "MRDIGFSTGFSLYDFSSSTGLRINYRFYKEKDERFYPDNQLFWPLVLELERSSCLRVDFSYLVAPSKCLNLVINPVRISEPHALVIVRNTSEQSISFSYLKGYLQEEYQKE", "aa_hexdigest": "98f082b0e8d77b0392eb166aa8b49bbc", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13094.704499999989, "isoelectric_point": 5.8510854721069325 }, "id": "NKOCPLAKPK_1992", "locus": "GCA003260355_01985" }, { "type": "cds", "sequence": "contig_3", "start": 37376, "stop": 37609, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4KG63", "UniRef:UniRef90_A0A328EAY0" ], "nt": "ATGCTCTGGAACGATATCATGACCGTGTTGAGAAAAATCTTTCATTTTCTTAAGTATGGAGGATGGAAAAAAGTGATAGGTTCCATCGTCCTTTTGCTACTCTTTGCTAATCTTTCTTTGATTAGCTTTTATACACGGTTTGCTTTTGAGGAACGGAGTCCGCAAGAAAGGAGTCAGTATTTTGGGTGTGATTACAACAAGAAGAAACGGCAACAAACGCTTTGTATTGCTTGA", "aa": "MLWNDIMTVLRKIFHFLKYGGWKKVIGSIVLLLLFANLSLISFYTRFAFEERSPQERSQYFGCDYNKKKRQQTLCIA", "aa_hexdigest": "e8cc09c235a3e302b5f980ccdfd58a80", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 37310, "stop": 37609, "observations": { "mutation": [ 37378 ], "stop": [ 37378 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EAY0", "query_cov": 1.0, "subject_cov": 0.7777777777777778, "identity": 1.0, "score": 157.0, "evalue": 1.25e-48, "gene_start": 1, "gene_end": 77, "reference_start": 1, "reference_end": 77, "reference_sequence": "MLWNDIMTVLRKIFHFLKYGGWKKVIGSIVLLLLFANLSLISFYTRFAFEERSPQERSQYFGCDYNKKKRQQTLCIAGDLQLEVFRRLHTLITSPFETK" }, "paralog": false, "identity": 0.98989898989899, "score": 200.0, "evalue": 4.66e-68, "description": "Internal stop codon at 37378. Nonsense mutation around 37378.", "psc": { "uniref90_id": "UniRef90_A0A328EAY0", "uniref50_id": "UniRef50_A0A2W4KG63", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EAY0", "UniRef:UniRef50_A0A2W4KG63" ] }, "pscc": { "uniref50_id": "UniRef50_A0A2W4KG63", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4KG63" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 9214.804499999991, "isoelectric_point": 9.79824352264404 }, "id": "NKOCPLAKPK_1993", "locus": "GCA003260355_01986" }, { "type": "cds", "sequence": "contig_3", "start": 37817, "stop": 38422, "strand": "-", "frame": 3, "gene": null, "product": "DUF6261 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBF8436", "UniRef:UniRef100_A0A328ECY6", "UniRef:UniRef50_A0A328ECY6", "UniRef:UniRef90_A0A328ECY6" ], "nt": "ATGATTGACGCGAATGGGCAACTGAAAAAAGATCCCAATTCTGAGGAGATCAACAAGTTGGACCAAGAACGTGATCAGCTTTTAACTTATTTTTTTACTAAGATTGCTGCAGAAATGCGTTCCCCTGATCAAGCGTTGGAAGCGGCAGCAACTGCATTGGGGGATTTTTTGGTAGTGTATCGTGGGATTCAAGGGAAAGCGAACCGTCAGGAAACGCAGCTGATCAAGAGTTTACTTTTGGATGCTGAGCAGGCGAAGTATACCCCACATTTACAGGCTTTAAAGCTGAATGTGGTCTTGGCAAAGCTCAAAACTGTCAATGAAAGCTATGAAAATTTACTCGACACGCGCACGAATGAAGGGCAAGCGACATTGATGATCGTGACCAAACCCCTCCGCAAGGAACTTGACGAACACTATGAAACACTCAGCACGAAACTTTTTGCTGTGAATGTAGCGAGTCCTACACCTGAGAGCGAGAAAGCGATAGCGGATTTGAATCGTTTGATCAAAGCGACGAAATTGGCTTGGAGGCAGAGGAACGGGTTGGTGCATTTGCATCCTGAGGATTTGGAAGATGAAAAAGAGAACGAAAGTGAAGGATAA", "aa": "MIDANGQLKKDPNSEEINKLDQERDQLLTYFFTKIAAEMRSPDQALEAAATALGDFLVVYRGIQGKANRQETQLIKSLLLDAEQAKYTPHLQALKLNVVLAKLKTVNESYENLLDTRTNEGQATLMIVTKPLRKELDEHYETLSTKLFAVNVASPTPESEKAIADLNRLIKATKLAWRQRNGLVHLHPEDLEDEKENESEG", "aa_hexdigest": "083fdd756531f6e92a11b74d9d663d57", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000DBF8436", "uniref100_id": "UniRef100_A0A328ECY6", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBF8436", "UniRef:UniRef100_A0A328ECY6" ] }, "ips": { "uniref100_id": "UniRef100_A0A328ECY6", "uniref90_id": "UniRef90_A0A328ECY6" }, "psc": { "uniref90_id": "UniRef90_A0A328ECY6", "product": "Family of unknown function (DUF6261)", "uniref50_id": "UniRef50_A0A328ECY6" }, "pscc": { "uniref50_id": "UniRef50_A0A328ECY6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328ECY6" ] }, "genes": [], "id": "NKOCPLAKPK_1994", "locus": "GCA003260355_01987" }, { "type": "cds", "sequence": "contig_3", "start": 38427, "stop": 39758, "strand": "+", "frame": 3, "gene": null, "product": "Putative amidase domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EBK6", "UniRef:UniRef90_A0A328EBK6" ], "nt": "TTGACATTTGCTTTAAACGCTTTTCGTGTTCCGTCTGTAACCTCAGAGCAATCTAGAGGAACAGGAGAACTTTTAGCTTGGGAGAAGGCGAGGCACGATTACTCATACCTTGAAGGAAAGGTAGAAGAGGCAAATCAATACCTTACCAAGAACAAGCTAGAACTCCAAGATCTGAAACAGTTGCATGAGATAGGAAATCTTATCTTTGGAGATTATCTTTCTAAAATTATGATGGATTTTCAGAAGAGGAGCTTAGCGTGGCTTGATGATAAGCTTTTTATCAATGAGTATGCAAAAAATAACATGTATGCTTTTTTGATTATGGATTTTCATGATGATAAGAGGTATTCTAAAGAGGTGTTGCAATCTTCTCCAGATATTCATATCATGACAAATCACATTGGCGAGAGAAATGGATGGGTCTTTATACCCATTAGAGTGTATTTCCCTGCTCCAGCCGAAGTTGCAGATGATCCCACATTTGATGAAAATGGTGGAAATGATACGAGATGGAGGTATTATGTGCTCCAACTCAAGAGAGACGAGAAATCACCTTATCATTGGAAGGTTGCTTATGAGGAACAAGTCGTAAGTCGTGGGATAGACGGAGAAATTCTGCGAGGTGCATGGTTTGCTACTCTGAATCCACAAGATGACTTTTCTGCTAAAATGTGGAAAGACGTTCTTTCAAGAAAGGATTATTGGGAATACTACATTGGAGAAAGAGACTGTCCTCGGACAGGAAGTATTCTCTGTCCTAAAAAAGAACCACTATCTCAATTAAGAAGTGGATTGTATGATAGACAAGCAGCAGCGCGCTATGCGTATTATCATGCATTATCTTATAATACTGAGTATGCAAAGTTTTCTCCTAATGATTGTGCTAATTTTGTTTCTCAGTGTCTCTATGCCGGAGGAATCCCCAAAGATAATACTTGGGGAACTCTTGATGGACGCTATCCTTATTATGGGACATCTACTTGGCTGGGAGCAAATGCTTTATACGAGTATATGAAGAAACATTATGGATATATTGTTTCTAAAGAGGATCTTGTGGCAGGATATGATGGGGACAGAGATTGTGTTCTAGAATGGGGCGACATGCTTGCAATGAATAGAAAAGGTGGAGTTGATATTGATGATGCTGTTAGAAAGACACATGTAATGATTATCTCCCATTCATATCATTACACAAATCAGCATTATAATGGAGTTCCTAGGTATAAGGTCTGCGGTCATACTAATAATAGATTGGATCTTGATATAATGTCACTTCCTGGAGGAAGGGAGTACGTGAAAAAACACATTATGGGCATCCATATTACCTACTGA", "aa": "MTFALNAFRVPSVTSEQSRGTGELLAWEKARHDYSYLEGKVEEANQYLTKNKLELQDLKQLHEIGNLIFGDYLSKIMMDFQKRSLAWLDDKLFINEYAKNNMYAFLIMDFHDDKRYSKEVLQSSPDIHIMTNHIGERNGWVFIPIRVYFPAPAEVADDPTFDENGGNDTRWRYYVLQLKRDEKSPYHWKVAYEEQVVSRGIDGEILRGAWFATLNPQDDFSAKMWKDVLSRKDYWEYYIGERDCPRTGSILCPKKEPLSQLRSGLYDRQAAARYAYYHALSYNTEYAKFSPNDCANFVSQCLYAGGIPKDNTWGTLDGRYPYYGTSTWLGANALYEYMKKHYGYIVSKEDLVAGYDGDRDCVLEWGDMLAMNRKGGVDIDDAVRKTHVMIISHSYHYTNQHYNGVPRYKVCGHTNNRLDLDIMSLPGGREYVKKHIMGIHITY", "aa_hexdigest": "9aa2d1446094c620d53f32243cf2a9df", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A328EBK6", "query_cov": 1.0, "subject_cov": 0.9910514541387024, "identity": 1.0, "score": 938.0, "evalue": 0.0, "valid": true, "product": "Putative amidase domain-containing protein", "uniref50_id": "UniRef50_A0A328EBK6" }, "pscc": { "uniref50_id": "UniRef50_A0A328EBK6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EBK6" ], "product": "Putative amidase domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_1995", "locus": "GCA003260355_01988" }, { "type": "cds", "sequence": "contig_3", "start": 39897, "stop": 40196, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGGAAAGGAGTAGTTTTATTAGGGATGCTTTCAAGTGCTTTGGCAGTGATGGGATGTTCATTTTCTCAACAGCCAGTTCCTCAAGAGGAAATGCTCTCTCATTCAGGGGAACAGCTTACTCAAGCATTCACCGGAGGAACCTGTGATACACCAAACCTTGCGGAATTGCAAAAGCCTATTCCTCCCTGTGAACCGTTAGATATCAAGCCTTTCCCTGAAGCGGACATCAAAAATATAGAGGATGTTGCAAGGAATTGTAAGTATCAGAAAGGACATTTCTTTTATGCTCCTTTTTGA", "aa": "MRKGVVLLGMLSSALAVMGCSFSQQPVPQEEMLSHSGEQLTQAFTGGTCDTPNLAELQKPIPPCEPLDIKPFPEADIKNIEDVARNCKYQKGHFFYAPF", "aa_hexdigest": "6de790e1c1d7f254a366f5e751e7f5fc", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10885.443299999986, "isoelectric_point": 5.212954139709471 }, "id": "NKOCPLAKPK_1996", "locus": "GCA003260355_01989" }, { "type": "cds", "sequence": "contig_3", "start": 40260, "stop": 40556, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCCCTATGATCCGGAAAAAGGAAAGGTTCTTTGCACTGACGGTGACTATGACTATGAGGAACTGAGACGCCCAAAAGAAAAAGCCTTAGAGTCCTTTGTAAAAAAGCACGATTTACAGCATAATCCGTATTTTACTTCCCTTCTCGTTAACTTGGACAATAATACAGAAGATGAAATCGTGATCCAACAGATGACAAAGTGTGAAGAAATTGAGCTTTGTAGAGATTTAGTCCCAAAAGTTCGAGGAGATAATGAACATCATTTCTTCTTTCATACGCAGGCTTCGATGACGTGA", "aa": "MPYDPEKGKVLCTDGDYDYEELRRPKEKALESFVKKHDLQHNPYFTSLLVNLDNNTEDEIVIQQMTKCEEIELCRDLVPKVRGDNEHHFFFHTQASMT", "aa_hexdigest": "ce2fad16b8b0bfac8da901194eb5e11d", "start_type": "GTG", "rbs_motif": "TTATT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11603.900099999993, "isoelectric_point": 4.9832677841186515 }, "id": "NKOCPLAKPK_1997", "locus": "GCA003260355_01990" }, { "type": "cds", "sequence": "contig_3", "start": 40596, "stop": 40715, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTATAGGCTTGGGACGAGCAGAGAAAGTGAAAGTTATAATGAGATTACAGAACGTTCTGCATGGGTCGTCCATGACCTAGTGGGGGACAAAATGATTGTAAAAAGGCTGACGAACTGA", "aa": "MYRLGTSRESESYNEITERSAWVVHDLVGDKMIVKRLTN", "aa_hexdigest": "42b9464f343daf4c99192b4b6f505f63", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4585.139699999999, "isoelectric_point": 6.536563682556152 }, "id": "NKOCPLAKPK_1998", "locus": "GCA003260355_01991" }, { "type": "cds", "sequence": "contig_3", "start": 41070, "stop": 42656, "strand": "+", "frame": 3, "gene": "alaS", "product": "alanine--tRNA ligase", "db_xrefs": [ "EC:6.1.1.7", "GO:0000166", "GO:0004813", "GO:0005737", "GO:0006419", "SO:0001217", "UniParc:UPI000DC006AD", "UniRef:UniRef100_A0A328EF36", "UniRef:UniRef50_A0A7W4ECQ1", "UniRef:UniRef90_A0A2W4IJ26" ], "nt": "ATGCAATCCCTTACTTCTCAGGAAATCAGACAAAGACGATCAGATTTTTGGACTTCTAAAGCACATACGCATTTACCAGAAGCCTCACTTATCGCAGACAAGGAATCTACTGCGCTTTTCAATGTTGCGGGAATGCAGCCGCTTATTCCTTATCTTGCTGGGAAAGAGCATCCGCTTGGACATAGGCTTTTTAATATCCAAAAATGCGTGAGAACGGTGGATATTGATGAGGTTGGAGATAGTTCACATCTAACTTTCTTTGAGATGATGGGAAACTGGAGCTTGGGTGATTATTTCAAGCAGGAGGCAATTCAATGGAGTTATGAGTTCCTCGTAGAGAAGCTGAATTTTGATCCTCGTAAATTAGCCGTAACCGTGTTTGAGGGGAATGAAGATGCTCCGAGAGATGAGATCGCTTTTGAGGCTTGGAAAAAAGCAGGGCTTCCTGAGGAGAGGATTTCTTTTTTGGATGCTAAGAATAACCGATGGAGTCCAGGTCCTGTTGGTCCTTGCGGTCCTGATACGGAGATTTACTACCGAGTGGGGGAATCAGAATTTCCACCTGTTGGTTCTAATGTAAAAACCGATGAAGCCAATTGGCTAGAAATCTGGAATAATGTATTTATGGAGTTTTATAGAGATGAAAAGGGAATCCTGACCAAGCTTTCTCAGCAGAATGTAGATACCGGAATGGGACTAGAAAGAATGTGTAAGGTTCTTCAGCACAAGGAAAGCGTTTATGAAACCGATCTTTTCGCCCCTTTCCTGCATATGCTAGAACAGGCAACCGAACTCAAGTATGCTGACCACCAGAGAAAATTCAGAATTATTGCTGATCATTTGAGAACAGCGTTTATGCTGATTAACGATGGGCTTACGCCTTCCAATGTCGGAGCAGGCTATGTTTTGAGGATGATTATCAGGAGAGGGTATTACAATCTCTTTTTGCTTAGAAAGATGATGAAGCCGGAGCTGGAGCATTTTATAGATCAGGCTTTCGTGGCGTTCAAAGGGCTTAGAGAGTTTAATGAGCCGATGATCAAAAAGGTGCTCCTTCAGGAGATCGCTCAGTTTGAGAAAACCATTCATAATGGAGAAAAAATTCTGACTGAACTCTTGGATAAGCTTACTACCGAAGGGAAGAAAACGCTCGGAGGAGATCAGATTTTTATGCTCTATGATACCTACGGTTTCCCATTAGAGATTACGAAAGAACTTGCTGCGGATCGTGGAGTTGCTTTGGATCTCGCAGGCTATGAAAAGGCTTTAGAAGCAGCAAAAGAGAAATCTCGCCAAGGAAGCAAGGCGATGTTCCAGAAATCTGCAGATTGGTCTAAGTATTTGGAGGGAGTTCCTGCGACAGAGTTTGTGGGGTATGAGAACCTGAATTTTTCTGATGCGAAGCTTTTGAAGGAGATTGATACCGAAGAGGGGCAGAAAGTGCTGATTTTTGATAAGACACCGTTCTATCCTGAGATGGGAGGTCAGAATGGAGATGCTGGAGTGATTGAGCTAGATGATGGTCGCCGTTTGGAGATTGTAAATGTGCAGAAGGTTGCAGGCGTGATTTTGCATTTTGTAGGGTAG", "aa": "MQSLTSQEIRQRRSDFWTSKAHTHLPEASLIADKESTALFNVAGMQPLIPYLAGKEHPLGHRLFNIQKCVRTVDIDEVGDSSHLTFFEMMGNWSLGDYFKQEAIQWSYEFLVEKLNFDPRKLAVTVFEGNEDAPRDEIAFEAWKKAGLPEERISFLDAKNNRWSPGPVGPCGPDTEIYYRVGESEFPPVGSNVKTDEANWLEIWNNVFMEFYRDEKGILTKLSQQNVDTGMGLERMCKVLQHKESVYETDLFAPFLHMLEQATELKYADHQRKFRIIADHLRTAFMLINDGLTPSNVGAGYVLRMIIRRGYYNLFLLRKMMKPELEHFIDQAFVAFKGLREFNEPMIKKVLLQEIAQFEKTIHNGEKILTELLDKLTTEGKKTLGGDQIFMLYDTYGFPLEITKELAADRGVALDLAGYEKALEAAKEKSRQGSKAMFQKSADWSKYLEGVPATEFVGYENLNFSDAKLLKEIDTEEGQKVLIFDKTPFYPEMGGQNGDAGVIELDDGRRLEIVNVQKVAGVILHFVG", "aa_hexdigest": "05b80aea46b28ca3cb746a0e3eb9ba50", "start_type": "ATG", "rbs_motif": "TTTTT", "ups": { "uniparc_id": "UPI000DC006AD", "uniref100_id": "UniRef100_A0A328EF36", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DC006AD", "UniRef:UniRef100_A0A328EF36" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EF36", "uniref90_id": "UniRef90_A0A2W4IJ26" }, "psc": { "uniref90_id": "UniRef90_A0A2W4IJ26", "gene": "alaS", "product": "alanine--tRNA ligase", "ec_ids": [ "6.1.1.7" ], "uniref50_id": "UniRef50_A0A7W4ECQ1", "go_ids": [ "GO:0000166", "GO:0004813", "GO:0005737", "GO:0006419" ] }, "pscc": { "uniref50_id": "UniRef50_A0A7W4ECQ1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7W4ECQ1" ], "product": "Alanine--tRNA ligase" }, "genes": [ "alaS" ], "id": "NKOCPLAKPK_1999", "locus": "GCA003260355_01992" }, { "type": "cds", "sequence": "contig_3", "start": 42684, "stop": 43139, "strand": "+", "frame": 3, "gene": null, "product": "Methyltransferase domain-containing protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBFE332", "UniRef:UniRef100_A0A328EBS8", "UniRef:UniRef50_A0A328EL41", "UniRef:UniRef90_A0A328EL41" ], "nt": "ATGCGATTAGAAGAAAAGCTAGCAACACTCTTCCATCACCAAAAAATTTCTGCAGTGCTTGATCTCGGAGCAGGCGATGGAAGGTGGAGTATTTTCTGTGCGAGCTATGGTGCGAAAGTAGTCGCAATCGACAATCAATCCAAGCCTGATCGGCAATTCCCTGAGTATTTGACTCTTCATCCAGCGATACATTTCTTTCAAGCTGATTTAAAGGAGTTGCCTCGTCCTTGCGACCAAAGATACGATCTAATCTTGCTCTTCAATGTTATCATCTTTCTCAAAAAGAGTTTTGTTCTCTCTCAATTGCTTCCTTATGCTCTTTCACAGCCGAATCCTGAAGGAAGACTTTGTTTGACCTTTTTCTTTTTCGATGATCAGACGATGGGAGTCAATCAAAAGCTTTCTTTCTATACCTTTGAGGATTTTCAGCCCAAAGACATCAGTGAAGAAATTTAG", "aa": "MRLEEKLATLFHHQKISAVLDLGAGDGRWSIFCASYGAKVVAIDNQSKPDRQFPEYLTLHPAIHFFQADLKELPRPCDQRYDLILLFNVIIFLKKSFVLSQLLPYALSQPNPEGRLCLTFFFFDDQTMGVNQKLSFYTFEDFQPKDISEEI", "aa_hexdigest": "518ac819d1b013984ca05a8db9c1d497", "start_type": "ATG", "rbs_motif": "TTTTTA", "ups": { "uniparc_id": "UPI000DBFE332", "uniref100_id": "UniRef100_A0A328EBS8", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBFE332", "UniRef:UniRef100_A0A328EBS8" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EBS8", "uniref90_id": "UniRef90_A0A328EL41" }, "psc": { "uniref90_id": "UniRef90_A0A328EL41", "product": "Methyltransferase domain-containing protein", "uniref50_id": "UniRef50_A0A328EL41" }, "pscc": { "uniref50_id": "UniRef50_A0A328EL41", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EL41" ], "product": "Methyltransferase domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_2000", "locus": "GCA003260355_01993" }, { "type": "cds", "sequence": "contig_3", "start": 43304, "stop": 43735, "strand": "-", "frame": 3, "gene": null, "product": "Polysaccharide pyruvyl transferase domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A288DFG0", "UniRef:UniRef90_A0A328EFK9" ], "nt": "ATGGATACGAGCTACTTTTGCTATCCTCGAGACAAGGTAGAGTCTCTTCCTCGTGCAGAAAAAACCGTCTTGATCAATCTAAACAAGAATGGGGAACAGTTTTTTGAAGAGCTAATAAAGGAGTGTGAAACTCTTATCGCTACAGGGTTTTCTCTTAAATATGTGCCTGTCAGCAAAGGCACTTCTCCTGTATACAACGATCTTTTGTACAAAAACAAGCTAGAAAAAAGACTTGGGATCACCTTGCCGATTGTGGATCGAGAGTCAGACTTTAATGCTTTTGTCCAAGAAGTAGCAGACGCAGAATTGGTGATTACAGCAAGGCTACACCTCTTCCTTGTTAGCTCTTTTATTGGGACGAAAGTAAAGGTTTTCCCTTACCAGAAAAAAATCCTGAAAATGCAAAAGATGCTCCAAATCATGCAATACTAA", "aa": "MDTSYFCYPRDKVESLPRAEKTVLINLNKNGEQFFEELIKECETLIATGFSLKYVPVSKGTSPVYNDLLYKNKLEKRLGITLPIVDRESDFNAFVQEVADAELVITARLHLFLVSSFIGTKVKVFPYQKKILKMQKMLQIMQY", "aa_hexdigest": "ddccc2d98324ff8a72fb24200d2d1be3", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 43304, "stop": 44035, "observations": { "mutation": [ 43759, 43927, 44005 ], "stop": [ 43759, 43927, 44005 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EFK9", "query_cov": 1.0, "subject_cov": 0.4281437125748503, "identity": 0.972, "score": 270.0, "evalue": 1.31e-88, "gene_start": 1, "gene_end": 143, "reference_start": 192, "reference_end": 334, "reference_sequence": "MPLAKTFQSDLPLKNKKVLILGNRSYKNMGDELILLGTIRILQEQNNEVIVSAYNPERLQSFFAQFPDLKPITYIHEFPKGFRSAFRYFFSDKRKELATYRGVDGVIIGGGEILTEESVHSYRYRNLGLLPLQAKLKKTPVYLMGGIQIPKKKINQMLFRWLLSQTEGVFARDPESVEALKDFGYHKTHFFMDTSYFCYPRDKVESLSHTEKTVLINLNKNGEQFFEELIKECEMLIATGFSLKYVPVSKGTSPVYNDLLYKNKLEKRLGITLPIVDRESDFNAFVQEVADAELVITARLHLFLVSSFIGTKVKVFPYQKKILKMQKMLQIMQY" }, "paralog": false, "identity": 0.9588477366255144, "score": 459.0, "evalue": 2.03e-165, "description": "Internal stop codon at 43759, 43927, 44005. Nonsense mutation around 43759, 43927, 44005.", "psc": { "uniref90_id": "UniRef90_A0A328EFK9", "product": "Polysaccharide pyruvyl transferase domain-containing protein", "uniref50_id": "UniRef50_A0A288DFG0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EFK9", "UniRef:UniRef50_A0A288DFG0" ] }, "pscc": { "uniref50_id": "UniRef50_A0A288DFG0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A288DFG0" ], "product": "Polysaccharide pyruvyl transferase domain-containing protein" } }, "id": "NKOCPLAKPK_2001", "locus": "GCA003260355_01994" }, { "type": "cds", "sequence": "contig_3", "start": 44003, "stop": 44308, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCTTTAGCAAAAACTTTCCAATCAGATTTGCCGTTAAAGAATAAAAAGGTTTTGATCCTCGGAAATCGGTCATATAAAAATATGGGTGACGAGCTTATTTTGCTGGGGACGATCCGTATTTTGCAGGAGCAGAATAACGAAGTAATCGTCTCTGCCTATAATCCAGAGCGATTGCAATCCTTTTTCGCACAATTCCCTGACCTAAAACCGATTACCTACATCCACGAATTTCCCAAAGGATTCCGTTCAGCCTTCCGCTATTTTTTTTCTGATAAGAGGAAAGAACTCGTGACTTACCGTTGA", "aa": "MPLAKTFQSDLPLKNKKVLILGNRSYKNMGDELILLGTIRILQEQNNEVIVSAYNPERLQSFFAQFPDLKPITYIHEFPKGFRSAFRYFFSDKRKELVTYR", "aa_hexdigest": "69ea93325bbba8052070f813f5a05cdb", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11933.728399999984, "isoelectric_point": 9.739835166931151 }, "id": "NKOCPLAKPK_2002", "locus": "GCA003260355_01995" }, { "type": "cds", "sequence": "contig_3", "start": 44412, "stop": 45173, "strand": "-", "frame": 2, "gene": null, "product": "Transglycosylase SLT domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4IGY1", "UniRef:UniRef90_A0A328EBJ9" ], "nt": "ATGAAAATCAGAAATTGGATTATGGTTATGAGTTGTATCGGGCTATTTGTTGGATGGACTGCAGCCTTTGAGCCAAGCACAGGAAATCAGGAGGAGCTTGCTGCTTTAAAGTCGCAGATTGCTCCCCTTGTTGAAAACGATAATCAAGCTCTACGAAGTCTCTATCAGCAGGCTAGAGATTTGCAAACACAGTTTAAGGAAGGATCAACTTCTTACTATCTTGAGAATTTGAGAGATTATTTATTCACAAAACTCTCAAGTAGAAAAGACATCGCAAAGGCTGAAAGCAGAACCTTCAAAGCCGGTTTTCTCTTGCCTTATCAGGCTTCAGGACTCTTGCTTGCAGAGCCGCTCCATGAGAATTGTATCGGTTGGTATCAAACCCTTGATAATCTGAGTTTCGCTTATGATTTCCCGACAGCTTTAACGATTGCAGTTTGGTACAGAGAAAATGGTTGTGGCTACTACTTGCCTAAAAATGGAGACGGTCCCTTCCAAATTGTGAGCAAAGACTACGGAACAGGAATGATTACAAGAGAGCTTTTCGAGACGACTATCAAGGATTTCCTTGAATTTTCAAAGAAAAAGATTGATCGATATAATGGAACGAATCCTACAACGCCCATCTCACTCAGTTACAAAAATTTTAGCACAGGCGACTTGCTAAAGTTTTCTGCTTTGTATAATGGACTCAGTGGAAGCTCTGTATCTGGAGATATTCTCCCAGCTGCTCCAAAATATTTCTATGAAAAAATGCCATGA", "aa": "MKIRNWIMVMSCIGLFVGWTAAFEPSTGNQEELAALKSQIAPLVENDNQALRSLYQQARDLQTQFKEGSTSYYLENLRDYLFTKLSSRKDIAKAESRTFKAGFLLPYQASGLLLAEPLHENCIGWYQTLDNLSFAYDFPTALTIAVWYRENGCGYYLPKNGDGPFQIVSKDYGTGMITRELFETTIKDFLEFSKKKIDRYNGTNPTTPISLSYKNFSTGDLLKFSALYNGLSGSSVSGDILPAAPKYFYEKMP", "aa_hexdigest": "2c9bd6370fbbe5013567c5c033cca5b2", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A328EBJ9", "query_cov": 1.0, "subject_cov": 0.9100719424460432, "identity": 0.96, "score": 492.0, "evalue": 1.87e-175, "valid": true, "product": "Transglycosylase SLT domain-containing protein", "uniref50_id": "UniRef50_A0A2W4IGY1" }, "pscc": { "uniref50_id": "UniRef50_A0A2W4IGY1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4IGY1" ], "product": "Transglycosylase SLT domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_2003", "locus": "GCA003260355_01996" }, { "type": "cds", "sequence": "contig_3", "start": 45350, "stop": 45640, "strand": "-", "frame": 3, "gene": null, "product": "sugar transferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E622E43" ], "nt": "ATGTATACACACTTAAGTGTAGGATATGGAGGAAAGGAAGCCGAGGAACTCTACAAAAAGCTTATCAATTCTGATGCAAATACTAGAGAGGGCGTCTTACCAAAGATTGCTAATGATCCGAGAGTCACCAAAGTGGGAAGATTCCTACGTAGGACCTCTTTAGATGAATTGCCACAGCTTTTCTCTGTGCTCAGAGGAACGATGTCGTTAGTTGGTCCAAGACCACACCTAGAAAACGAAGTCGCAAGATATACGCCTTGGCAAAGGAGGCTCCTTAGTATAAAACCTTGA", "aa": "MYTHLSVGYGGKEAEELYKKLINSDANTREGVLPKIANDPRVTKVGRFLRRTSLDELPQLFSVLRGTMSLVGPRPHLENEVARYTPWQRRLLSIKP", "aa_hexdigest": "56e1f3dfe04fe0a5820cb471b3edc0bf", "start_type": "ATG", "rbs_motif": "TTTTT", "psc": { "uniref90_id": "UniRef90_UPI001E622E43", "query_cov": 0.8541666666666666, "subject_cov": 0.9761904761904762, "identity": 0.5, "score": 83.6, "evalue": 3.13e-19, "valid": false, "product": "sugar transferase", "uniref50_id": "UniRef50_UPI001E622E43" }, "pscc": { "uniref50_id": "UniRef50_UPI001E622E43", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E622E43" ], "product": "sugar transferase" }, "genes": [], "id": "NKOCPLAKPK_2004", "locus": "GCA003260355_01997" }, { "type": "cds", "sequence": "contig_3", "start": 46142, "stop": 46573, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTAAACTCCGCCTTACGAGACCCGTTCTGCACTTTCTTATCATCAATTGCCTGTTCTTTCTTACCTACAAAGTCAGGTTTTGGACTGATTTTATCCCAGGAGTTCAGCTTCCGATTCCGTTTATCAATGCGACGGAGTTGATAATATTTTCAATCATTGCAAGTGCGGGGTTTATTGGTATTGGGATTATTAAGGATTTTTATTGCTTGAATAAGAGAGTCATCAACCATTTCCAGACCTTTTCCAAAGTGTGGATTTATTGGTTCATCAGTATCACGTTTCTCTCGTATTTTGGTCAGGGCTTTATCTTCTTTTTTGGGATTTCTAGGTTTATCCTTATTTTTACAGGGCTTTTGACCTATATTGTACTATTTTTCTTTGACCAAGGACGAAGAGCCCTAGAATATCAGCAACAGAAAAAATCCTGA", "aa": "MFKLRLTRPVLHFLIINCLFFLTYKVRFWTDFIPGVQLPIPFINATELIIFSIIASAGFIGIGIIKDFYCLNKRVINHFQTFSKVWIYWFISITFLSYFGQGFIFFFGISRFILIFTGLLTYIVLFFFDQGRRALEYQQQKKS", "aa_hexdigest": "c88ad325cfc1199cace0dce05c5bd714", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17033.150399999984, "isoelectric_point": 9.95187168121338 }, "id": "NKOCPLAKPK_2005", "locus": "GCA003260355_01998" }, { "type": "cds", "sequence": "contig_3", "start": 46850, "stop": 47476, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAACCGGTTTATAATTTGCTTGAATACCTTGATGCTGATAAGGTGTACACCTTTAGTGATGAACTTGATAATCAAATTGAAATCAAAGTATATATTCAGATTCATAGTGAAAAGTTTGTGGAGTATCAGAAAGAGATTTTGCAAGAGTTTGTAGCTGATTTTACAGACAAAGCGAAGGATGTATCTCTTTCTTCCAGTGATATTGAAATGATGCTTGAAGGACAGCTTCAACTCCTCAATACTAAGCTTCAATCTTTTGCGGATAAGCTCCGTGATGCTCCGAAATGCGACTTGAGAGGGTATGTGCAGCTGATCTTAGATAATAGCGTGAAGACCTGGATGATTGGGAAGACTACGCTTATGATTTTCCGTAATGATAAACTCTACTCTGTCCTAGAAAATTCATTCCAAGAACAGACTAACATTGATCAGTTTTCTGATTTTATTGGTGGGGAACTAGAAAGAGGAGATGTTTTTCTCTATGCTGGGACGAAGCTTTCAGAAGTCTTGGATCAGAACGACTTTTCAGAGATGGAACAAGTACTTGCCAAAGAAAATGCAAATGCGATGCTCGATCATCTTGATGATCTCTTTGCTTCGAGAGTAGAAAAGAAAGGAATCTGA", "aa": "MKPVYNLLEYLDADKVYTFSDELDNQIEIKVYIQIHSEKFVEYQKEILQEFVADFTDKAKDVSLSSSDIEMMLEGQLQLLNTKLQSFADKLRDAPKCDLRGYVQLILDNSVKTWMIGKTTLMIFRNDKLYSVLENSFQEQTNIDQFSDFIGGELERGDVFLYAGTKLSEVLDQNDFSEMEQVLAKENANAMLDHLDDLFASRVEKKGI", "aa_hexdigest": "d57c6014462fa01495caa23c36e07b22", "start_type": "ATG", "rbs_motif": "TTATT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 24056.965500000002, "isoelectric_point": 4.451539039611816 }, "id": "NKOCPLAKPK_2006", "locus": "GCA003260355_01999" }, { "type": "cds", "sequence": "contig_3", "start": 47486, "stop": 48328, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTCAGCATCACAGGGATTGATCTCTCTACGATTAAACCTAAAGGGAAGCTCTCTGCCGTGGCATCAAAATATACTTCTAAACTCGGCGGAAAGCTTGCATCAAAGGTTGGCTTGATGAAGCGAAAGAAACAGACCAGTCAACTCATTAGCGGGCATAAGTACTATCTTATGGTAGGGGTGCTTGTTGTTGCTATTCTTTTCCTTGGAGGTGCGGTCCTTTCTCAGCTAAAGTCTGCAAAGAAAGAAGAAGTGTATTTCAAGAATGCTAGTGGAAGTCAGGAACTCATTACTATTGATAGCATTAGAAATGATATCCTTGCATTCCAAGCTATGGATCCTTCCTCAGATGAGAAATCCCTTGAATATAACAGAATCTTAAGCAAGCTCAGTCTACTTGCTGAAAAGGGGCTTTGGGAAAAAGATGTGAAAGATCTCAAGAACATCATTGATACGAATTATCAGAAAGGCTTTAATGTGAGTTTGATTACAAATCTCTCTCAGTTTGATGATGAAGCAACCGGGAGAAAAACCAGAATCCTTACTTTTAATGATGCGGAAAAGGCAAAGCTCGGGACTCCAATCAGTATTAATATTGGGGCAACGACAAATATCGGAGGAACCCAGGCCGCTCTCATTGGTGTAATAAATGATGCTACAAGAGGAAATCTGATCGAATATAATGTTGGCTCTGCGATGAAAGACTGCGAATTGAGCTTGGGGACAAGAGGACTCTTTTGTTATGCTGAGAATGGGGATTTATTCTTCGTGAACAAGCTTGGTGTTGAGCAAATGGAGGTCGTAGATGAAGACCGAGCTACGAAAAATATTGGGGGCATCTGA", "aa": "MFSITGIDLSTIKPKGKLSAVASKYTSKLGGKLASKVGLMKRKKQTSQLISGHKYYLMVGVLVVAILFLGGAVLSQLKSAKKEEVYFKNASGSQELITIDSIRNDILAFQAMDPSSDEKSLEYNRILSKLSLLAEKGLWEKDVKDLKNIIDTNYQKGFNVSLITNLSQFDDEATGRKTRILTFNDAEKAKLGTPISINIGATTNIGGTQAALIGVINDATRGNLIEYNVGSAMKDCELSLGTRGLFCYAENGDLFFVNKLGVEQMEVVDEDRATKNIGGI", "aa_hexdigest": "869cd75c64a0bac9cc1bdfeeefabaf91", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 30434.71830000003, "isoelectric_point": 8.9656343460083 }, "id": "NKOCPLAKPK_2007", "locus": "GCA003260355_02000" }, { "type": "cds", "sequence": "contig_3", "start": 48581, "stop": 48967, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGATTACCGCGAGGTTCCGATCATTGGAGGAGATAAGGTTGGTTCGCTCTACTCAGATAACGTAAAAATTATTGCTACGGATAGTTCTCCATTTATTTACCTCTTTGACAAAGATAATCAAACCTTTACCGTCTATGAATCAAGTCCTGTAAAGACGAACGACAACTACAAGACGAACTTCAGACTTTATTATCTTTTTAGATTTAAGTTTAACTTGGCTGGGACAGAGAACCGTATTTTGGATGTAGCTGTTCCAGAAAGTACTGCAGATAGACCAGAACTCCATCTCCTCAGTAATGAAGGTGTAAATAAAATCAATCTCTACGATTTCATTGATAGTATCAAAAATAATAAGAATCTGAAATCTGTGAACAATGCGTCATAA", "aa": "MDYREVPIIGGDKVGSLYSDNVKIIATDSSPFIYLFDKDNQTFTVYESSPVKTNDNYKTNFRLYYLFRFKFNLAGTENRILDVAVPESTADRPELHLLSNEGVNKINLYDFIDSIKNNKNLKSVNNAS", "aa_hexdigest": "fe9ef45a3df87e1438f44ff01e509ef8", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14659.22229999998, "isoelectric_point": 5.349765586853027 }, "id": "NKOCPLAKPK_2008", "locus": "GCA003260355_02001" }, { "type": "cds", "sequence": "contig_3", "start": 48998, "stop": 49531, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MZ92", "UniRef:UniRef90_A0A660MZ92" ], "nt": "ATGAAAGGCATCAAATTCGGAGTCATCCTTCTGCTTTTGCTCCTTATTGGAGGAGGAATCTGCTGTAAACGAAGTGAACTCAAAGAATTTTTTATCGTGCATGCTCCGACACATGAGGAAGAAGTTCTTTCTGCCAGAGGGATCGAAACTCCTCAGCAAATCTTTTCTGGGATCTCAATTAAAGCTATGGATGATCTTGGACTCACTGGCGAGGAAGCTGTGGATGCTTACTTGATGGCGAATCTCCCGAGATGCCACTTCCCAGAGCTCAAAAAAAACAGTAAACCGATTGTCATCGACGAAGTAGTAAAGAACTGCAAGAAGATACAAAAGGTTGGTAGAGTCCCTGATGGATTAGGAGGGGCTTGTTATCCCTCCTTCGCTTATGATCGTGTCGGAGACAGGGTATTGGAGATGAACGCAAGAGAATGCATCTATGGCATCAATCCTGCTGAATACCTCGCTCGCGGAGTCAATCCTAGCTACCTTTGCTTAGACCCGCAAACTCCTGAGAGAGATTGGGATGAGATTTGA", "aa": "MKGIKFGVILLLLLLIGGGICCKRSELKEFFIVHAPTHEEEVLSARGIETPQQIFSGISIKAMDDLGLTGEEAVDAYLMANLPRCHFPELKKNSKPIVIDEVVKNCKKIQKVGRVPDGLGGACYPSFAYDRVGDRVLEMNARECIYGINPAEYLARGVNPSYLCLDPQTPERDWDEI", "aa_hexdigest": "abbaaff59a27a67fceeb79d5a691aa52", "start_type": "ATG", "rbs_motif": "TTCTT", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 48998, "stop": 49834, "observations": { "mutation": [ 49529, 49574, 49817 ], "stop": [ 49529, 49574, 49817 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A660MZ92", "query_cov": 1.0, "subject_cov": 0.5236686390532544, "identity": 0.983, "score": 330.0, "evalue": 1.24e-111, "gene_start": 1, "gene_end": 177, "reference_start": 1, "reference_end": 177, "reference_sequence": "MKGIKFGVILLLLLLIGGGICCKRSELKEFFIVHAPTDEEEVLSARGIETPQPIFSGISIKAMDDLGLTGEEAVDAYLMANLPRCHFPELKKNSKPIVIDEVVKNCKKIQKVGRVPDGLGGACYPSFAYDSVGDRVLEMNARECIYGINPAEYLARGVNPSYLCLDPQTPERDWDEIGELAPEERDEPDGKPGKAPYSLLALQKALKSGLLSKAVPPYYNTPGLFCAEKDPDCETTYDIGSRSDTQGNYFVAGERTDLPDKKLYAINDQLYLPGEGSIGFSSENGELSGQRTTPWFFGGIKEGNLIVKRMIDMTPNGKFIPGGGMEANYSLETCEIDL" }, "paralog": false, "identity": 0.9748201438848921, "score": 522.0, "evalue": 6.89e-190, "description": "Internal stop codon at 49529, 49574, 49817. Nonsense mutation around 49529, 49574, 49817.", "psc": { "uniref90_id": "UniRef90_A0A660MZ92", "uniref50_id": "UniRef50_A0A660MZ92", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A660MZ92", "UniRef:UniRef50_A0A660MZ92" ] }, "pscc": { "uniref50_id": "UniRef50_A0A660MZ92", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MZ92" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 19653.639099999993, "isoelectric_point": 5.459123802185059 }, "id": "NKOCPLAKPK_2009", "locus": "GCA003260355_02002" }, { "type": "cds", "sequence": "contig_3", "start": 49613, "stop": 49819, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAATCAGGACTCCTAAGCAAGGCTGTTCCTCCTTACTATAATACCCCTGGACTTTTTTGTGCAGAGAAGGATCCTGATTGCGAAACGACCTACGATATTGGCTCTCGAAGCGATACCCAAGGGAATTACTTCGTGGCTGGAGAGAGGACAGACCTGCCTGACAAGAAACTCTATGCTATCAATAATCAGCTTTACCTTCCTTGA", "aa": "MKSGLLSKAVPPYYNTPGLFCAEKDPDCETTYDIGSRSDTQGNYFVAGERTDLPDKKLYAINNQLYLP", "aa_hexdigest": "20cfe7fbdef8fc029e4bd2e91bbc068f", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7595.420400000002, "isoelectric_point": 4.764949226379394 }, "id": "NKOCPLAKPK_2010", "locus": "GCA003260355_02003" }, { "type": "cds", "sequence": "contig_3", "start": 50798, "stop": 51316, "strand": "+", "frame": 2, "gene": null, "product": "Glycosyl transferase family 4", "db_xrefs": [ "EC:2.7.8.13", "KEGG:K01000", "SO:0001217", "UniRef:UniRef50_A0A2W4IZF6", "UniRef:UniRef90_A0A328ECZ5" ], "nt": "ATGTTTGAAGGAGCATCGGTATTGGTTAAATTAAGCCAAATCCTCTTTTATGCGCTAGTGAGTTTTGGGATTTCACTCTTGCTCTATCCTGCGTATATCAGGTTGCTTCAAAGAGTAAAAGCAGGAAAAACGATCCGCGAAAATGCGGCAACAGGAGAAAAGTCTGAGATCTTCAGCAAAATGCATGCCCACAAACAAGGCACACCAACCATGGGAGGAGGAATGTTTTTGATCGTGATGTTGGTGATGATTCTGGGGTCGTTTTTGCTCCAACATTTTGGATGGATCGGCAACTCGCTCTGGAATGCAAAAGAATCCTATATCATTTTGTTTGGATTTTTTAGTATGGGATTGATTGGGCTGGTTGACGACTTTCTCAACATCAAGAATTATGGGAAAGTGAAGGGATTGAGTGCGAGAGCGAAGTTGATTGGAATGGTGATTTTCTCGGCTTGGATTTCCTATTGGTTTTATGTGAAGCTCGGGATTGATTATATCAATCTTTGGCCAATCGCATGA", "aa": "MFEGASVLVKLSQILFYALVSFGISLLLYPAYIRLLQRVKAGKTIRENAATGEKSEIFSKMHAHKQGTPTMGGGMFLIVMLVMILGSFLLQHFGWIGNSLWNAKESYIILFGFFSMGLIGLVDDFLNIKNYGKVKGLSARAKLIGMVIFSAWISYWFYVKLGIDYINLWPIA", "aa_hexdigest": "728c361c226e5321f5d54495c3d9c61f", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 50798, "stop": 51619, "observations": { "mutation": [ 51314, 51329 ], "stop": [ 51314, 51329 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328ECZ5", "query_cov": 1.0, "subject_cov": 0.479108635097493, "identity": 1.0, "score": 335.0, "evalue": 1.51e-113, "gene_start": 1, "gene_end": 172, "reference_start": 1, "reference_end": 172, "reference_sequence": "MFEGASVLVKLSQILFYALVSFGISLLLYPAYIRLLQRVKAGKTIRENAATGEKSEIFSKMHAHKQGTPTMGGGMFLIVMLVMILGSFLLQHFGWIGNSLWNAKESYIILFGFFSMGLIGLVDDFLNIKNYGKVKGLSARAKLIGMVIFSAWISYWFYVKLGIDYINLWPIAGKIEVGILFPILTFIATITIVNAINITDGLDGLAGGTMAIILVSLAIVTFLNGTYIATTVVCILVAVLVAFLFFNINPAKVFMGDSGAFALGGLLASLIYLLNMRFGILIPFLIIFGIFIVELCSSFLQIFWKKVFKKKLFTVAPFHHLLEYRGMHETTIVMKTRLVQAILAIAGVLLIFVESSLMK" }, "paralog": false, "identity": 0.9926739926739927, "score": 526.0, "evalue": 5.2e-191, "description": "Internal stop codon at 51314, 51329. Nonsense mutation around 51314, 51329.", "psc": { "uniref90_id": "UniRef90_A0A328ECZ5", "product": "Glycosyl transferase family 4", "ec_ids": [ "2.7.8.13" ], "uniref50_id": "UniRef50_A0A2W4IZF6", "kegg_orthology_id": "K01000", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328ECZ5", "EC:2.7.8.13", "UniRef:UniRef50_A0A2W4IZF6", "KEGG:K01000" ] }, "pscc": { "uniref50_id": "UniRef50_A0A2W4IZF6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4IZF6" ], "product": "Phospho-N-acetylmuramoyl-pentapeptide-transferase" } }, "id": "NKOCPLAKPK_2011", "locus": "GCA003260355_02004" }, { "type": "cds", "sequence": "contig_3", "start": 51335, "stop": 51634, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTCCTATTTTGACTTTCATTGCGACGATCACGATCGTGAATGCAATCAACATTACTGACGGATTAGATGGCTTGGCTGGCGGCACGATGGCGATTATTTTGGTGAGTCTCGCAATTGTGACCTTTTTGAATGGAACCTATATTGCGACGACAGTAGTTTGCATTCTTGTTGCGGTTTTGGTTGCGTTTTTGTTTTTTAATATCAACCCGGCGAAAGTTTTTATGGGGGATAGTGGTGCGTTTGCACTCGGAGGGCTACTTGCCTCTCTGATCTATCTGCTCAATATGAGATTCTGA", "aa": "MFPILTFIATITIVNAINITDGLDGLAGGTMAIILVSLAIVTFLNGTYIATTVVCILVAVLVAFLFFNINPAKVFMGDSGAFALGGLLASLIYLLNMRF", "aa_hexdigest": "a87ccc90c807fbb60868c75764db362c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10403.474299999989, "isoelectric_point": 4.427837181091309 }, "id": "NKOCPLAKPK_2012", "locus": "GCA003260355_02005" }, { "type": "cds", "sequence": "contig_3", "start": 51638, "stop": 51877, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTCCTTTTTTGATTATTTTTGGGATTTTTATTGTCGAGCTTTGTTCGAGTTTTCTCCAGATTTTTTGGAAAAAGGTTTTTAAGAAAAAACTCTTTACGGTAGCACCGTTCCATCATCTTTTGGAATACAAGGGAATGCATGAAACAACGATCGTTATGAAGACTCGACTGGTGCAAGCAATTCTCGCAATCGCTGGAGTATTATTGATTTTTGTAGAATCATCCTTGATGAAATAA", "aa": "MIPFLIIFGIFIVELCSSFLQIFWKKVFKKKLFTVAPFHHLLEYKGMHETTIVMKTRLVQAILAIAGVLLIFVESSLMK", "aa_hexdigest": "5e5431f320ff0202e5f72f7e5cc6150c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9158.233199999995, "isoelectric_point": 9.731905555725099 }, "id": "NKOCPLAKPK_2013", "locus": "GCA003260355_02006" }, { "type": "cds", "sequence": "contig_3", "start": 51882, "stop": 52088, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAATCCTCCTCAAACAATCCGTTACTTCTATCAAATCACTCAATCAGCTCCTCGGACTTTCGGAAGCAGAACAGCAAAGACTAGTTTCTGTCGGAGTCGGAGAAGGTTTGATGTTCGTCGGAAACCAGCACGTCGGCGTCCAAATCTTAGCTTCCCCTTATGAAAAATCTTTTATTACCACCGATATTAAAAAAAGCGAATAA", "aa": "MQILLKQSVTSIKSLNQLLGLSEAEQQRLVSVGVGEGLMFVGNQHVGVQILASPYEKSFITTDIKKSE", "aa_hexdigest": "82261ab1681e3056f417a9abf54acdef", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7417.513899999999, "isoelectric_point": 6.556400489807129 }, "id": "NKOCPLAKPK_2014", "locus": "GCA003260355_02007" }, { "type": "cds", "sequence": "contig_3", "start": 52170, "stop": 52535, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGACGACTACACGGGGAAACTTCCTCCGCTGATGGAAGATCTGATGAATGTCTTGAATGGAATGCAGGGAGGAGAGCAAATCGCACTTAAGCTCAGTAAATATGTAACTGGAACCTTCGGAAAACTCTTCAACAATTACACGAATATTGACATCAATAATGCGCTTACGGTCTTTAGTATCCGTGATGTGGAAGAAGCTCTGAAAACCCCTGCGATGTTTAATGTGCTTAATTTTATTTGGTCCAAGGTGAGATCGGTCAAAAAGCAGAGACTTCTCGTCTGTGATGAAGCGTGGATTATGCTTCAAAACGATATTTCTGCAAACTTCCTCTTCGGTCTTATCAAAAGAGCCAGAAAATACTGA", "aa": "MDDYTGKLPPLMEDLMNVLNGMQGGEQIALKLSKYVTGTFGKLFNNYTNIDINNALTVFSIRDVEEALKTPAMFNVLNFIWSKVRSVKKQRLLVCDEAWIMLQNDISANFLFGLIKRARKY", "aa_hexdigest": "f0be91f970e39c1d2c0d137bb40b9d10", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13871.074799999986, "isoelectric_point": 9.067429924011229 }, "id": "NKOCPLAKPK_2015", "locus": "GCA003260355_02008" }, { "type": "cds", "sequence": "contig_3", "start": 52614, "stop": 52907, "strand": "-", "frame": 2, "gene": "traG", "product": "TraG P-loop domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A351Y302" ], "nt": "ATGAATAGTGTGATTTTGGCGACTTCTGGTGCCGGTAAATCTTTTACGGTGAAGCTAGAGATTCTCAGGTATTTGCTCAATGGAGTAGATATTATTGTGATTGATCCTGAAAACGAATATCAAGCGCTTTGTGAAAGAGTAGGCGGAACCTATGTGAATATTGCAACTTCTTCTCAGCAGTTTCTCAATCCTTTTGATATGCCACCGATGATTGAAGATGTGGAGTATGGAAAGGGAGATTTGCTTCGTTCGCAGATTATGAATCTCGTGGGCTTGATTCAGATTTTGGTTTGA", "aa": "MNSVILATSGAGKSFTVKLEILRYLLNGVDIIVIDPENEYQALCERVGGTYVNIATSSQQFLNPFDMPPMIEDVEYGKGDLLRSQIMNLVGLIQILV", "aa_hexdigest": "014d62e5635d8b43fe8f78dcc9f84651", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A9E5GMR6", "query_cov": 0.8041237113402062, "subject_cov": 0.8571428571428571, "identity": 0.833, "score": 139.0, "evalue": 3.3e-41, "valid": false, "product": "DUF87 domain-containing protein", "uniref50_id": "UniRef50_A0A351Y302" }, "pscc": { "uniref50_id": "UniRef50_A0A351Y302", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A351Y302" ], "product": "TraG P-loop domain-containing protein" }, "genes": [ "traG" ], "id": "NKOCPLAKPK_2016", "locus": "GCA003260355_02009" }, { "type": "cds", "sequence": "contig_3", "start": 52980, "stop": 53108, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATAATGGATTTACAGCGAATTTACCGATCTGCCTTGATGATATGGGAATCGTTCGTAGTGCGGTGACTTCTTCGTTGGCAGCCAGCTTTCCTTTTATTTCTAGTGATCTTATTACCAAAACCTGA", "aa": "MDNGFTANLPICLDDMGIVRSAVTSSLAASFPFISSDLITKT", "aa_hexdigest": "1c8e4a0c8b5da912db9aafc802836dfa", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4421.0311999999985, "isoelectric_point": 4.138697242736816 }, "id": "NKOCPLAKPK_2017", "locus": "GCA003260355_02010" }, { "type": "cds", "sequence": "contig_3", "start": 53220, "stop": 53690, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1V5ZNA0" ], "nt": "GTGAAGCAACTTTTACAGCTTCCAGATGCGGAATATAGCGACAAAAAGCTGACTTCCGAGCTCAGACAATATATCAAAGAGGTTGATAATCGTTATTCAGCTTTACTTTCTGATTATAAATCACATATTGCACCGTCCTACCGAGAGTTTACGATGCAGCAGTTTAATGTATCTGGAATCCTCGGAAAATGCTATTATACTCAGGAATATCCGAGTTATATTGATGCACTCTGGACGAGGGATATTATGAGCATGCATGCCAAGCGAGATATGAGTTTCTACCTCTATCCTGAGGATGATTCGGCGATCCAGTCTATGCTTAAGCAAAAATCCACCCAGCTCAAAGCCGAGCTTTCTGATGCGATGCAGAAGGGAATTACGATTGACAAGGAAATTGAACAGCAATACCGTGATGTAGAGATGATCAGAGAAAAACTGACGACCAAAGAGGAAAGATACTTTGAACTCTGA", "aa": "MKQLLQLPDAEYSDKKLTSELRQYIKEVDNRYSALLSDYKSHIAPSYREFTMQQFNVSGILGKCYYTQEYPSYIDALWTRDIMSMHAKRDMSFYLYPEDDSAIQSMLKQKSTQLKAELSDAMQKGITIDKEIEQQYRDVEMIREKLTTKEERYFEL", "aa_hexdigest": "26eca8aaabdfb8f7f85e91e4eb140f55", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A9E5GT14", "query_cov": 0.967948717948718, "subject_cov": 0.848314606741573, "identity": 0.722, "score": 226.0, "evalue": 2.99e-73, "valid": false, "uniref50_id": "UniRef50_A0A1V5ZNA0" }, "pscc": { "uniref50_id": "UniRef50_A0A1V5ZNA0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1V5ZNA0" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18621.944000000003, "isoelectric_point": 5.304123878479003 }, "id": "NKOCPLAKPK_2018", "locus": "GCA003260355_02011" }, { "type": "cds", "sequence": "contig_3", "start": 53775, "stop": 53972, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTGAGAAGCTACAAAACATTGTCTATGATACTAATTACACTACCATAGTTCCGAATGGAGGTTTTGTAGTTCTCCCAAAAAACACACCGCAATCTTCAGGAGAAGCACCAAAACCCGAGAAAAAAAACTCGATGGCATGGATGGATTCCTTACTTCAGAAACTCGGACTTGCTCCAGCTCCAAAAAAGCCTTGA", "aa": "MLEKLQNIVYDTNYTTIVPNGGFVVLPKNTPQSSGEAPKPEKKNSMAWMDSLLQKLGLAPAPKKP", "aa_hexdigest": "2623344a4c27f7e22e0035717716ac42", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7126.234800000002, "isoelectric_point": 9.399055290222169 }, "id": "NKOCPLAKPK_2019", "locus": "GCA003260355_02012" }, { "type": "cds", "sequence": "contig_3", "start": 54150, "stop": 54476, "strand": "-", "frame": 2, "gene": null, "product": "Tyr recombinase domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3D5YIY2" ], "nt": "ATGGAAAAACTATATACCTACTTAAACTTGAGAACTGATACCTATGAACCACTCATTATCAGCCTTTCCAAAAATAGCTATGGAAAACAGCTTTCCAGAAATGCAGTAGAGGCATTAGTCAAAAATTATGCCAAACTTTCTGGAATCGAAAAGAAAGTCACGCCTCATACGCTGAGACATTCTTTTGCCACTACGTTGCTCAAGAAAGGAGCCGATATTAGATCCGTTCAAACACTGCTTGGGCATTCTTCTATTCAAACAACTCAGATCTATACCCACGTAGATGATCATTTCCTCAAAGGAGTTCATGATTTATTGGAAGAATAA", "aa": "MEKLYTYLNLRTDTYEPLIISLSKNSYGKQLSRNAVEALVKNYAKLSGIEKKVTPHTLRHSFATTLLKKGADIRSVQTLLGHSSIQTTQIYTHVDDHFLKGVHDLLEE", "aa_hexdigest": "422f577afa912f140b55e0f70cc98894", "start_type": "ATG", "rbs_motif": "TTTTTA", "psc": { "uniref90_id": "UniRef90_A0A351Y6U2", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.6759999999999999, "score": 142.0, "evalue": 3.65e-42, "valid": false, "product": "Tyr recombinase domain-containing protein", "uniref50_id": "UniRef50_A0A3D5YIY2" }, "pscc": { "uniref50_id": "UniRef50_A0A3D5YIY2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3D5YIY2" ], "product": "Tyr recombinase domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_2020", "locus": "GCA003260355_02013" }, { "type": "cds", "sequence": "contig_3", "start": 54543, "stop": 54860, "strand": "-", "frame": 2, "gene": "xerC", "product": "Tyrosine recombinase XerC", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1V5ZM58" ], "nt": "ATGGCACTTAATAATTTAGGACTTGCAAAAAAGACCGTTAATTATCATATCATTGCCTTGAGATCGTTCTTAAGGTTTCTCCTCAAAAATGATATTGATTGCCTCAGTCCAGACAAAATAGAACTTTCAAAAATACCACAGAGAGAGGTAAGTTTTTTACTGGAAAATGAAATAGATAAAATTCTGAGCGCACCAATTCATTTCGCGACTTCAGAACTGCAAAAGGCAAGAGATGAATTGATTCTCCATATTCTTTATGGGACAGGATTGAGAGTCTCAGAATTAATAAGCCTCAAAAAGAGTCAGCTCATTCTCTGA", "aa": "MALNNLGLAKKTVNYHIIALRSFLRFLLKNDIDCLSPDKIELSKIPQREVSFLLENEIDKILSAPIHFATSELQKARDELILHILYGTGLRVSELISLKKSQLIL", "aa_hexdigest": "1e5a8bb128913ef8f7409e2f81198074", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A1V5ZM58", "query_cov": 0.9809523809523809, "subject_cov": 0.8583333333333333, "identity": 0.621, "score": 137.0, "evalue": 8.98e-40, "valid": false, "product": "Tyrosine recombinase XerC", "uniref50_id": "UniRef50_A0A1V5ZM58" }, "pscc": { "uniref50_id": "UniRef50_A0A1V5ZM58", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1V5ZM58" ], "product": "Tyrosine recombinase XerC" }, "genes": [ "xerC" ], "id": "NKOCPLAKPK_2021", "locus": "GCA003260355_02014" }, { "type": "cds", "sequence": "contig_3", "start": 54909, "stop": 55130, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02899.23" ], "nt": "ATGGCTAACAAAAACTTTATCACAAGTTCTTCATGGACTGGATATCATCAGAAAAAAGAGGGGCCAAAACCTTCTTTTTCTGAAACTCCAAAAAAAGGACTATACAAGATGTCTGCGTTACTGACGACATTTCTTGATTATCTGGAACATACTAAGAATGTAAGCCCAAAAACAATAGTGAATTACCGCTTATGGCTCCAAAGAGCTGTTGAATTTCTCTGA", "aa": "MANKNFITSSSWTGYHQKKEGPKPSFSETPKKGLYKMSALLTTFLDYLEHTKNVSPKTIVNYRLWLQRAVEFL", "aa_hexdigest": "be1589340ba18fe2f791a5a1125a6122", "start_type": "ATG", "rbs_motif": "TTTA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02899.23", "name": "Phage_int_SAM_1", "length": 32, "aa_cov": 0.4383561643835616, "hmm_cov": 1.0, "evalue": 6.769411180294353e-06, "score": 22.789386749267578, "start": 42, "stop": 73 } ], "seq_stats": { "molecular_weight": 8497.7138, "isoelectric_point": 9.7943754196167 }, "id": "NKOCPLAKPK_2022", "locus": "GCA003260355_02015" }, { "type": "cds", "sequence": "contig_3", "start": 55123, "stop": 55266, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATCAAGTATGGATTAGCAGTAGAAAAAGAGTCTGAAAAAATCTTGGATAGGCTTGCAAATGGTGAAAATATTGATATATCTACATGGTCAGAATTAAAATCCCAGCTTGATGACTATTTAACTAAAGCAACAAATGGCTAA", "aa": "MIKYGLAVEKESEKILDRLANGENIDISTWSELKSQLDDYLTKATNG", "aa_hexdigest": "7ebbd14b736c6daea122529fdf6e6b24", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5301.8869, "isoelectric_point": 4.606538963317871 }, "id": "NKOCPLAKPK_2023", "locus": "GCA003260355_02016" }, { "type": "cds", "sequence": "contig_3", "start": 55288, "stop": 55575, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAGACTTTAGATGATGGAGAGATTCAGCAAACAGATCCTGTTTCTGTTTCCGGAACCTTTGACGATGATAATGAAGACGCTATCCAAACTATTGATCTCTCTCAAACAAATACTCCATCTACCACAACAAATCCTTCAACGGGGAACGCAGCTGCTTCAGGAGTAAACAATGCAGATGGACTTGGAGCAATAGAGAATCTCGTAGGTCCTGTTAATAATAATGACATTCTTAAGCAAGAGGTAATGGAATTTCAGAGAAAAGGAAAGGAAGTAAGGGAGATGTGA", "aa": "MQTLDDGEIQQTDPVSVSGTFDDDNEDAIQTIDLSQTNTPSTTTNPSTGNAAASGVNNADGLGAIENLVGPVNNNDILKQEVMEFQRKGKEVREM", "aa_hexdigest": "96221a417df81f54222c3b4cae8d13bc", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10125.776, "isoelectric_point": 4.0500284194946286 }, "id": "NKOCPLAKPK_2024", "locus": "GCA003260355_02017" }, { "type": "cds", "sequence": "contig_3", "start": 55627, "stop": 56391, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAAGGAGAAGAAAACATTTCTCTTACTGATGCAATTCCTTCAACAGAAACCCTCTCACCAAATGAAATATCAGAAGCTAAAGATGAGGCAGTATTTCTTGAAGATGCCTTACCTACGGCAAATGAAAGTGTCCCAGAAGAAGTTAATCAAGACGCTCCTTCACAGATGTTTATGGACGCATCTCCTGAAAGTGAAGCTAACACTCAACCTTCTGAACCATTTATGACGAGCGGCCAATCCGCTGAAAATCAGCCAATCCAGCAGGAGCAGCCAACAGAAATGCCTTCATCCCTATCTGTACCAGCAGGAGATGGAAATTTGCTTAATCTTGATGAGATGCTAGCCCAATCGCCTCAAGATGTTTCTAATTCTACTTCAGATACTGCTGTTGATCCTTTTACCGCAATGAAAGAAACTCTTCAGGCGCAGCAGCAACCTACTCAGGCACCTACACTTTCTCCTATGTCAGAAACTGGAGCACTGAATGAAACCTTACAGACTCCTTCTCAGGAAAGTCTAGCATCTGATCCTCAAGCTCAATCACTAAATCTGGATATGTTATCTGCCCAGCTTCCTCCCCAGATAGCACCTCTCACAGCTTCATCGCAAACTCCAAAGCTAACAACTACCAACTTACCTCTAAAGAAAATGCTTGGAGTAGTATTTGGAGCCGTAATAGTAATCGCTACAGGGGTAATTGCTTATATTAGATATCCAGACCTGTTTTCTCTCAGCGCTCCACAAGGAGAGATGAGTTTATGA", "aa": "MQGEENISLTDAIPSTETLSPNEISEAKDEAVFLEDALPTANESVPEEVNQDAPSQMFMDASPESEANTQPSEPFMTSGQSAENQPIQQEQPTEMPSSLSVPAGDGNLLNLDEMLAQSPQDVSNSTSDTAVDPFTAMKETLQAQQQPTQAPTLSPMSETGALNETLQTPSQESLASDPQAQSLNLDMLSAQLPPQIAPLTASSQTPKLTTTNLPLKKMLGVVFGAVIVIATGVIAYIRYPDLFSLSAPQGEMSL", "aa_hexdigest": "13ba3c5ba25fdda372d7502b86c97fa7", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 26951.550099999993, "isoelectric_point": 4.0500284194946286 }, "id": "NKOCPLAKPK_2025", "locus": "GCA003260355_02018" }, { "type": "cds", "sequence": "contig_3", "start": 56437, "stop": 56799, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCGAGCCTTAACGCCCAAGACGAAACACCTCCTGCAGACCCTATGCCAAGTCAGGAGGTTAGCGATGGTTTTTTAAATGAAGAATCTACTCCTTTTGTAGGAGTTGAAAATCAGCAACAAAATCCAGAAAGTAGTGAGATGGTATCACCAGAAAATAATACTGACTTTTTACTCGAAGAAGCTCCTTCTGACCAAGAGGTTCCATTGATGATGTCAGAAGAGCCTTTACAGGAAGAAACAGTGCCTTCATCAGAGTCAGATTTCCCTACGAATGAATTTTTACAAGATGACCTATCACAAACAACCGCACAAGAGCAGACTTCTCCAGAAGAAGCTGGAAGTATGTTTTCCGCTACGTGA", "aa": "MPSLNAQDETPPADPMPSQEVSDGFLNEESTPFVGVENQQQNPESSEMVSPENNTDFLLEEAPSDQEVPLMMSEEPLQEETVPSSESDFPTNEFLQDDLSQTTAQEQTSPEEAGSMFSAT", "aa_hexdigest": "9497ecf13b3768af1b37105957f35c70", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13159.805499999995, "isoelectric_point": 4.0500284194946286 }, "id": "NKOCPLAKPK_2026", "locus": "GCA003260355_02019" }, { "type": "cds", "sequence": "contig_3", "start": 56926, "stop": 57102, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAATTTTATTCATTCAGTGTATAAAAATGCCAACTACGGATTCAACCATGACTCCACAATCCCCAGCAGTAGCTCAACCTCCAGTTCAAAACGACGATTTTGATCTTGGTATAACGCAAGAAATACCACAGGAAGCTCCTTCTACGCAGTTTTCTTCTGAAAATGTAGGATGA", "aa": "MKILFIQCIKMPTTDSTMTPQSPAVAQPPVQNDDFDLGITQEIPQEAPSTQFSSENVG", "aa_hexdigest": "9df9a6a141e9f076d18fd540a6c30604", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6324.0622, "isoelectric_point": 4.0500284194946286 }, "id": "NKOCPLAKPK_2027", "locus": "GCA003260355_02020" }, { "type": "tRNA", "sequence": "contig_3", "start": 57163, "stop": 57233, "strand": "-", "gene": "trnR", "product": "tRNA-Arg(ccg)", "amino_acid": "Arg", "anti_codon": "ccg", "score": 61.7, "nt": "AAGAAAGTGGTGTAGTGGTAGCACGTCAGTCTCCGAAGCTGAAAGTGGAGGTTCGATTCCTCTCTTTCTTG", "db_xrefs": [ "SO:0001036" ], "anti_codon_pos": [ 57199, 57201 ], "id": "NKOCPLAKPK_39", "locus": "GCA003260355_02021" }, { "type": "cds", "sequence": "contig_3", "start": 57477, "stop": 57800, "strand": "-", "frame": 2, "gene": null, "product": "Phosphoribosyltransferase domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EEP6", "UniRef:UniRef90_A0A328EEP6" ], "nt": "TTGACGCAGCTTAATCAAGATCTTACCCTTGCCATGCAAAAGGATCAGCTTTTTATCTCTTTTGTTCCTTCGCATCGGTATCGTAAATATTTTCAGAAGGGATATAATCAATCTGAACTCCTAGCAAAAGAACTAGCAACTCAGCTTGAGATGCCTCGAGTTCCTTGCTTCAAAAAGGTAAAGGCTACGACCTCTCAGGTGAAACTTACGAGATCAGAGAGATTGAAAAATTTGAATGCTGCATTCGTTTTCCTTCCTTCTGCAATAAGCTTGCTCCCTCAAAATGCAACCTTGCTCATCGTAGATGATGTGACGACGACCTGA", "aa": "MTQLNQDLTLAMQKDQLFISFVPSHRYRKYFQKGYNQSELLAKELATQLEMPRVPCFKKVKATTSQVKLTRSERLKNLNAAFVFLPSAISLLPQNATLLIVDDVTTT", "aa_hexdigest": "0a548782209ffd69307530748ad2527c", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 57387, "stop": 58100, "observations": { "mutation": [ 57479, 57809, 58043 ], "stop": [ 57479, 57809, 58043 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EEP6", "query_cov": 1.0, "subject_cov": 0.45147679324894513, "identity": 0.972, "score": 197.0, "evalue": 6.95e-62, "gene_start": 1, "gene_end": 107, "reference_start": 101, "reference_end": 207, "reference_sequence": "MHKWLEYLFPRSCYGCDKPGAYLCFECKKELFPHPELCPFCHKESKNFKTCSSCRFEGANFLDGLVVGFSYKTLLKKLILKLKFHHKSDITGFLAERGALLTQLNPDLTLAMQKDQLFISFVPSHRYRKYFQKGYNQSELLAKELATQLEIPRVPCFKKVKATTSQVKLTRSERLKNLNAAFVFLPSAISLLPQNATLLIVDDVTTTGATLNELAKRAKSSRPDLHIWGLVLARHTG" }, "paralog": false, "identity": 0.9704641350210971, "score": 468.0, "evalue": 2.36e-171, "description": "Internal stop codon at 57479, 57809, 58043. Nonsense mutation around 57479, 57809, 58043.", "psc": { "uniref90_id": "UniRef90_A0A328EEP6", "product": "Phosphoribosyltransferase domain-containing protein", "uniref50_id": "UniRef50_A0A328EEP6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EEP6", "UniRef:UniRef50_A0A328EEP6" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EEP6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EEP6" ], "product": "Phosphoribosyltransferase domain-containing protein" } }, "id": "NKOCPLAKPK_2028", "locus": "GCA003260355_02022" }, { "type": "cds", "sequence": "contig_3", "start": 58135, "stop": 58257, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAACCGATGGTAGTTCCTTATGTTGCACGAATTCCTATGATTGGCTGTATATCAGCGATTATTAGTTTAGTTGCTACTTTGCAAATCAACCTCCGCTTTAGCTTCAAAAAAGATGCATGA", "aa": "MQPMVVPYVARIPMIGCISAIISLVATLQINLRFSFKKDA", "aa_hexdigest": "108fb77bb9992e9239d00123adb759bb", "start_type": "ATG", "rbs_motif": "TTCTA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4436.4181, "isoelectric_point": 9.782061958312983 }, "id": "NKOCPLAKPK_2029", "locus": "GCA003260355_02023" }, { "type": "cds", "sequence": "contig_3", "start": 58324, "stop": 58533, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAAGGTATTCCAGATGAGACCTTTCTAGGACTCCGCTGTGCAAAAATTGCAGAATTTATCATCAATGCTCAGCCGACCATACTCCTCATATTTTTTGTGGCAGGGCTACGATTCTTTATCCAATACGGACACTTAAGCATCAAAATCTTTTCAGAAATTTTTGAAAAAAAACTCCAGACTTTGCTTCGATGTGTTTTAAGTTTTTGA", "aa": "MQGIPDETFLGLRCAKIAEFIINAQPTILLIFFVAGLRFFIQYGHLSIKIFSEIFEKKLQTLLRCVLSF", "aa_hexdigest": "06e885ec0f142d20c8bceb8121addd28", "start_type": "TTG", "rbs_motif": "TTTA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7952.487699999994, "isoelectric_point": 8.768554496765137 }, "id": "NKOCPLAKPK_2030", "locus": "GCA003260355_02024" }, { "type": "cds", "sequence": "contig_3", "start": 58610, "stop": 58756, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCTCTATATGTAGTTATCTTTCCCAAAGATGCAATAGTTTTTATTCAGATTGATCATTGTGATGTCTACAGAACGAAGCCTTGTGCTCTCACTTATCTTAGTTGTCGCGTTACTTTCGTTGCTAAAGGTCGAAAAGCTGGTTAG", "aa": "MPLYVVIFPKDAIVFIQIDHCDVYRTKPCALTYLSCRVTFVAKGRKAG", "aa_hexdigest": "3199e22ca9814aa6f3cbc3315f377a42", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5448.496, "isoelectric_point": 9.25645122528076 }, "id": "NKOCPLAKPK_2031", "locus": "GCA003260355_02025" }, { "type": "cds", "sequence": "contig_3", "start": 58832, "stop": 59062, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTAGAGGTTATCCTTGTTCTGATGATCATATCTACCGCATTGATAAGTGTAATTACTGGAGTTGCGAGAACAGTAAACTACGTCTCAGAGATGAAGCAAAGAACCCTAGCACTTAACCTTGCGAAAGAAGGAATCGAAACGGTATATAATATTAGAGATACTAATTGGAGAAGATGGTCTGCGACCAAGGATAAATGCCGACTTAAGGTTACCCCACTTGATGGATGA", "aa": "MLEVILVLMIISTALISVITGVARTVNYVSEMKQRTLALNLAKEGIETVYNIRDTNWRRWSATKDKCRLKVTPLDG", "aa_hexdigest": "bee7f998ae6e7733b37ce51859dac88b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8622.114499999992, "isoelectric_point": 9.649257087707518 }, "id": "NKOCPLAKPK_2032", "locus": "GCA003260355_02026" }, { "type": "cds", "sequence": "contig_3", "start": 59766, "stop": 59990, "strand": "-", "frame": 2, "gene": "rplK", "product": "50S ribosomal protein L11", "db_xrefs": [ "GO:0003735", "GO:0005840", "GO:0006412", "KEGG:K02867", "SO:0001217", "UniRef:UniRef50_A0A288DFG2", "UniRef:UniRef90_A0A660MP73" ], "nt": "ATGGAGATCGGTGCTCCAATCACTTCAGGACTCATCAAACGAAGAATCAATCAAAATCTTGGATCTGGAGAGCCAAACAAGAAAAAGATCGGAAAGCTTACCAAAGCAGATCTTGAAGCAATTTATGAACTCAAGAAGGCAGATCTAAACGCAAATACTCCAGAGGAAGCCTACAAAATTATTGCAGGAACCGCAAGAAATATGGGAGTTGAGGTAGAAATGTAA", "aa": "MEIGAPITSGLIKRRINQNLGSGEPNKKKIGKLTKADLEAIYELKKADLNANTPEEAYKIIAGTARNMGVEVEM", "aa_hexdigest": "f91b4b980bff3bee16052ece6d024da3", "start_type": "ATG", "rbs_motif": null, "genes": [ "rplK" ], "truncated": "3-prime", "pseudogene": { "start": 59766, "stop": 60206, "observations": { "mutation": [ 60050 ], "stop": [ 60050 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A660MP73", "query_cov": 1.0, "subject_cov": 0.5068493150684932, "identity": 0.865, "score": 125.0, "evalue": 2.03e-35, "gene_start": 1, "gene_end": 74, "reference_start": 73, "reference_end": 146, "reference_sequence": "MAKVQKILNIEVPAGKATPTPPLGPMLGANGVNIGQFTKEFNEKTADLMKQFGGIEVKIKVKLTVFVDRTFTMEIGAPITSGLIKWRISQNLGSGEPNKKKVGKLTLADLEAIYELKKSDLNANDKEAAFKIIAGTARNMGVDVEM" }, "paralog": false, "identity": 0.910958904109589, "score": 260.0, "evalue": 4.18e-91, "description": "Internal stop codon at 60050. Nonsense mutation around 60050.", "psc": { "uniref90_id": "UniRef90_A0A660MP73", "gene": "rplK", "product": "50S ribosomal protein L11", "uniref50_id": "UniRef50_A0A288DFG2", "kegg_orthology_id": "K02867", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A660MP73", "UniRef:UniRef50_A0A288DFG2", "KEGG:K02867", "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_A0A288DFG2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A288DFG2" ], "product": "Large ribosomal subunit protein uL11" } }, "id": "NKOCPLAKPK_2033", "locus": "GCA003260355_02027" }, { "type": "cds", "sequence": "contig_3", "start": 60048, "stop": 60206, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03946.19" ], "nt": "ATGGCAAAAGTACAAAAAATCTTGAACATCGAAGTTCCTGCTGGAAAGGCAACACCTACACCTCCGCTTGGACCTATGCTTGGTGCAAATGGAGTAAATATCGGGCAGTTTACCAAAGAATTTAACGAAAAAACTGCAGATTTGATGAAGCAGTTTTGA", "aa": "MAKVQKILNIEVPAGKATPTPPLGPMLGANGVNIGQFTKEFNEKTADLMKQF", "aa_hexdigest": "c1683d88a269c7811e45c4ab504f28f4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03946.19", "name": "Ribosomal_L11_N", "length": 40, "aa_cov": 0.7692307692307693, "hmm_cov": 1.0, "evalue": 2.884863665877047e-19, "score": 65.08934020996094, "start": 9, "stop": 48 } ], "seq_stats": { "molecular_weight": 5585.519700000001, "isoelectric_point": 9.303642082214353 }, "id": "NKOCPLAKPK_2034", "locus": "GCA003260355_02028" }, { "type": "cds", "sequence": "contig_3", "start": 60219, "stop": 60410, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3D0UWB6" ], "nt": "ATGAGCTCCGAAGCGGAGATACTCAAGAATAAACTATTTGGAGAAAGTATTAGCTTTGTCAATATCCCAGAATACAAAAATAAAATAGTAATTATGCTACTTACCGTTGATGATCAGTATTGGCTCATTCAGATGGACTATAAAAAATATCATCACGAGAAACCATATCTCAAGAAATTATTTATCTCTTAA", "aa": "MSSEAEILKNKLFGESISFVNIPEYKNKIVIMLLTVDDQYWLIQMDYKKYHHEKPYLKKLFIS", "aa_hexdigest": "95b536019f4b044f9b67c95d799b834d", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A948C4B7", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.54, "score": 82.8, "evalue": 9.87e-20, "valid": false, "uniref50_id": "UniRef50_A0A3D0UWB6" }, "pscc": { "uniref50_id": "UniRef50_A0A3D0UWB6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3D0UWB6" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7612.877700000001, "isoelectric_point": 7.950773048400879 }, "id": "NKOCPLAKPK_2035", "locus": "GCA003260355_02029" }, { "type": "cds", "sequence": "contig_3", "start": 60702, "stop": 60920, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAAAAGAAAAAAAGCTCGTAACATTATCAATGGTAGTGCTTTTCTTTAGTTTAATTGTGGTGTATTCATTATTCTATCAGATAAAAGGAGAAAAGCTGATCCAGATCTTGCGTGCAGCCTCCGCAACTTCTGCTTCTCAAGAGAGCAAACAGGAAAGCGGTGGACAGGAAGATTTACATATTGATTTGCTATCAGGCGATACGCAAGCGACTTGA", "aa": "MEKEKKLVTLSMVVLFFSLIVVYSLFYQIKGEKLIQILRAASATSASQESKQESGGQEDLHIDLLSGDTQAT", "aa_hexdigest": "08d5e39e81070c72c36e27a371c794c6", "start_type": "ATG", "rbs_motif": "TTCTT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7964.080299999995, "isoelectric_point": 5.187660789489745 }, "id": "NKOCPLAKPK_2036", "locus": "GCA003260355_02030" }, { "type": "cds", "sequence": "contig_3", "start": 60995, "stop": 61267, "strand": "-", "frame": 3, "gene": "nusG", "product": "transcription termination/antitermination protein NusG", "db_xrefs": [ "GO:0032784", "KEGG:K02601", "SO:0001217", "UniRef:UniRef50_K1XI85", "UniRef:UniRef90_A0A660MQA5" ], "nt": "ATGAACGATAAAATCTGGTATGTAGTGAGAAATACTCCAGGAGTAAGACTTATCGTAGGGGCTGAAACGAAGCCAATTCCTCTTTCAGAACAGGAGTATCAAGATATGATGAGACAGATTACTCAGTCTCAGGAAAGATCTGAGCTAAATATCCCTTACAAAATCGGGGATGTAGTCCTCCTAAAGCATGGTGATTTCAAGGGAATGAAAGGAAATATCAAAGAGCTTGATCCTGAAAAAGGAATGGTTGTAGTAAATATAGAGATGCTCTGA", "aa": "MNDKIWYVVRNTPGVRLIVGAETKPIPLSEQEYQDMMRQITQSQERSELNIPYKIGDVVLLKHGDFKGMKGNIKELDPEKGMVVVNIEML", "aa_hexdigest": "07a388ac252f6a78c70f4d6fadf079a4", "start_type": "ATG", "rbs_motif": null, "genes": [ "nusG" ], "truncated": "both", "pseudogene": { "start": 60944, "stop": 61525, "observations": { "mutation": [ 60997, 61327 ], "stop": [ 60997, 61327 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A660MQA5", "query_cov": 1.0, "subject_cov": 0.46632124352331605, "identity": 1.0, "score": 181.0, "evalue": 1.05e-56, "gene_start": 1, "gene_end": 90, "reference_start": 87, "reference_end": 176, "reference_sequence": "MQKEANTNEIKKQIEDRSFRRYVLSVTSGQEALVIENLQERINKQGLSEDVIDYLCPMINETSIKKGEKVIRQKKLYPGYVFFKSRMNDKIWYVVRNTPGVRLIVGAETKPIPLSEQEYQDMMRQITQSQERSELNIPYKIGDVVLLKHGDFKGMKGNIKELDPEKGMVVVNIEMLGRLTPVAISIDQVELVN" }, "paralog": false, "identity": 0.9896373056994818, "score": 374.0, "evalue": 7.33e-135, "description": "Internal stop codon at 60997, 61327. Nonsense mutation around 60997, 61327.", "psc": { "uniref90_id": "UniRef90_A0A660MQA5", "gene": "nusG", "product": "transcription termination/antitermination protein NusG", "uniref50_id": "UniRef50_K1XI85", "kegg_orthology_id": "K02601", "go_ids": [ "GO:0032784" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A660MQA5", "UniRef:UniRef50_K1XI85", "KEGG:K02601", "GO:0032784" ] }, "pscc": { "uniref50_id": "UniRef50_K1XI85", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_K1XI85" ], "product": "Transcription termination/antitermination protein NusG" } }, "id": "NKOCPLAKPK_2037", "locus": "GCA003260355_02031" }, { "type": "cds", "sequence": "contig_3", "start": 61325, "stop": 61525, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAAAAGAAGCAAATACCAATGAAATCAAAAAACAGATCGAAGATAGGAGTTTTAGACGATACGTCTTGAGTGTAACATCTGGACAAGAAGCTCTAGTGATCGAAAACCTTCAGGAGAGGATAAATAAACAGGGCTTGTCAGAGGATGTGATCGACTATCTCTGCCCTATGATCAACGAGACTTCAATCAAAAAATGA", "aa": "MQKEANTNEIKKQIEDRSFRRYVLSVTSGQEALVIENLQERINKQGLSEDVIDYLCPMINETSIKK", "aa_hexdigest": "6bf6a905200248a6cbca46b1669acef7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7686.667199999997, "isoelectric_point": 5.364089012145995 }, "id": "NKOCPLAKPK_2038", "locus": "GCA003260355_02032" }, { "type": "cds", "sequence": "contig_3", "start": 61560, "stop": 61922, "strand": "-", "frame": 2, "gene": "secE", "product": "Preprotein translocase subunit SecE", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DC01052", "UniRef:UniRef100_A0A328EF15", "UniRef:UniRef50_A0A328EF15", "UniRef:UniRef90_A0A328EF15" ], "nt": "ATGTTAGATTTTATTTATGATAGTTTAGATACCGTCAAAAAGCTTAAATTCCCAACAGTTAAGCAGATCGTAGGGTTAACGTTCGCTATTTTTGGTCTAGTAATTGTTGCAGGAGCGTACTTTATTTTCGCAGATACGCTTTTTACAGGAGGATACAAGGCTTTTTATGCAGCAATGACAGATGGACAGACAACCACTTCTGGAGCTGTAGCTTCAGGGGAAGCAAAAAATCCGATTCTTCCAGCACTTAATATTTCTGGGCTTTCTTTAGACAGTGCAGAAGCCTCACAGGCGGATGCTCAAGGCACCATACAAGATATAGACATGACTGAATCAGGAGCTGAAAGTTCGCTTTCAGAATAA", "aa": "MLDFIYDSLDTVKKLKFPTVKQIVGLTFAIFGLVIVAGAYFIFADTLFTGGYKAFYAAMTDGQTTTSGAVASGEAKNPILPALNISGLSLDSAEASQADAQGTIQDIDMTESGAESSLSE", "aa_hexdigest": "0eb39e1dd8135bdce44f5662ad65b47e", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000DC01052", "uniref100_id": "UniRef100_A0A328EF15", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DC01052", "UniRef:UniRef100_A0A328EF15" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EF15", "uniref90_id": "UniRef90_A0A328EF15" }, "psc": { "uniref90_id": "UniRef90_A0A328EF15", "product": "Preprotein translocase subunit SecE", "uniref50_id": "UniRef50_A0A328EF15" }, "pscc": { "uniref50_id": "UniRef50_A0A328EF15", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EF15" ], "product": "Preprotein translocase subunit SecE" }, "genes": [ "secE" ], "id": "NKOCPLAKPK_2039", "locus": "GCA003260355_02033" }, { "type": "cds", "sequence": "contig_3", "start": 62004, "stop": 62141, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATGCTCAAAGAATCTTTTCTAACCAGACAGAAAAAACAGATCGAACAAGATCTTTCAGAGGATAATGAGAGATTCAATGTCCTTGTTGAAGCAATGAGTGAAGTCGTAAAAGAATATATGGACAAAAAAACATAA", "aa": "MMLKESFLTRQKKQIEQDLSEDNERFNVLVEAMSEVVKEYMDKKT", "aa_hexdigest": "d7b9b1335c6eb7974e4eaa5fcc3e0176", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5440.207900000001, "isoelectric_point": 4.962578392028808 }, "id": "NKOCPLAKPK_2040", "locus": "GCA003260355_02034" }, { "type": "cds", "sequence": "contig_3", "start": 62148, "stop": 62426, "strand": "-", "frame": 2, "gene": null, "product": "DUF304 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7W4E337", "UniRef:UniRef90_A0A660MQH3" ], "nt": "GTGATAGGACTTGCTCTCTTTGCTAAGTGGACTATTGATTTTCTGAATAGTTATCTTGATTGTCTAATTCTCTCAAAAGATTCTCTTACTCTTTTTCTCTGGGAAGGACTTTTGGAATACAAAACTGAGGTTTTCTCTCGAAATAAAATCACAACGATTTCTCGAAGTCAGAACGGGATCTCAGACAAACTTTTTGCCAAAGGCGACCTGTTAATAAAATTAGAATTTGATACCGAGTTCCCTTTTACCGATATCGATGCTCCAAAAAAACAGCTTTGA", "aa": "MIGLALFAKWTIDFLNSYLDCLILSKDSLTLFLWEGLLEYKTEVFSRNKITTISRSQNGISDKLFAKGDLLIKLEFDTEFPFTDIDAPKKQL", "aa_hexdigest": "d0b96e6a6500a5e642db9ca240875762", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 62004, "stop": 62612, "observations": { "mutation": [ 62150, 62429 ], "stop": [ 62150, 62429 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A660MQH3", "query_cov": 1.0, "subject_cov": 0.45544554455445546, "identity": 0.9890000000000001, "score": 182.0, "evalue": 7.47e-57, "gene_start": 1, "gene_end": 92, "reference_start": 63, "reference_end": 154, "reference_sequence": "MLRNVLIKRYVDTKDIQRVIGHSSVVHVKRAIVMIALLFVLYVIFALLHKTTPAEYRKRIFGVIGLALFAKWTIDFLNSYLDCLILSKDSLTLFLWEGLLEYKTEVFSRNKITTISRSQNGISDKLFAKGDLLIKLEFDTEFPFTDIDAPKKQLGKLMMLKESFLTRQKKQIEQDLSEDNERFNVLVEAMSEVVKEYMDKKT" }, "paralog": false, "identity": 0.9900990099009901, "score": 386.0, "evalue": 1.03e-139, "description": "Internal stop codon at 62150, 62429. Nonsense mutation around 62150, 62429.", "psc": { "uniref90_id": "UniRef90_A0A660MQH3", "product": "DUF304 domain-containing protein", "uniref50_id": "UniRef50_A0A7W4E337", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A660MQH3", "UniRef:UniRef50_A0A7W4E337" ] }, "pscc": { "uniref50_id": "UniRef50_A0A7W4E337", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7W4E337" ], "product": "DUF304 domain-containing protein" } }, "id": "NKOCPLAKPK_2041", "locus": "GCA003260355_02035" }, { "type": "cds", "sequence": "contig_3", "start": 62427, "stop": 62612, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTGAGAAATGTACTCATCAAAAGATATGTAGATACCAAAGACATCCAACGAGTAATTGGGCATAGTTCCGTAGTGCATGTAAAGAGAGCAATTGTGATGATTGCTCTGCTTTTTGTTTTATACGTAATTTTTGCTCTGCTGCATAAAACAACTCCTGCAGAATATCGAAAGCGGATTTTTTGA", "aa": "MLRNVLIKRYVDTKDIQRVIGHSSVVHVKRAIVMIALLFVLYVIFALLHKTTPAEYRKRIF", "aa_hexdigest": "99796bb8970e980bdbe9adc647d776f3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7194.692299999997, "isoelectric_point": 10.714081382751463 }, "id": "NKOCPLAKPK_2042", "locus": "GCA003260355_02036" }, { "type": "cds", "sequence": "contig_3", "start": 62662, "stop": 62892, "strand": "-", "frame": 1, "gene": "rpmE", "product": "50S ribosomal protein L31", "db_xrefs": [ "GO:0003735", "GO:0005840", "GO:0006412", "SO:0001217", "UniParc:UPI000DC01325", "UniRef:UniRef100_A0A660MNU5", "UniRef:UniRef50_A0A2M7XIU8", "UniRef:UniRef90_A0A660MNU5" ], "nt": "ATGAAAAAAGGGATTCACGGAAAATACTTCAAAGACGTTGAAGTAAATTGCATTTGCGGAGCATCATTTACAGTAAATACCACTGTTGAAGGACCAATTAAGGTGGAAACTTGCTATCAGTGTCATCCAGTATTCAATAAGGATAGAGTAGTGACAAAGCCAGTTAAAGGAATGATGGAAAAATACCTTGAAAAGCAGAAGAGAATTGAGGCAGCTAAAAATAATAAATAG", "aa": "MKKGIHGKYFKDVEVNCICGASFTVNTTVEGPIKVETCYQCHPVFNKDRVVTKPVKGMMEKYLEKQKRIEAAKNNK", "aa_hexdigest": "bfe91634e6046a17c4f8b9c1c3955491", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000DC01325", "uniref100_id": "UniRef100_A0A660MNU5", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DC01325", "UniRef:UniRef100_A0A660MNU5" ] }, "ips": { "uniref100_id": "UniRef100_A0A660MNU5", "uniref90_id": "UniRef90_A0A660MNU5" }, "psc": { "uniref90_id": "UniRef90_A0A660MNU5", "gene": "rpmE", "product": "50S ribosomal protein L31", "uniref50_id": "UniRef50_A0A2M7XIU8", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_A0A2M7XIU8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2M7XIU8" ], "product": "50S ribosomal protein L31" }, "genes": [ "rpmE" ], "id": "NKOCPLAKPK_2043", "locus": "GCA003260355_02037" }, { "type": "cds", "sequence": "contig_3", "start": 63004, "stop": 63171, "strand": "-", "frame": 1, "gene": null, "product": "RNHCP domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3D0UWT2" ], "nt": "ATGTATCCGACGAGTTATGAAATGAAAAATAGTGAAATCAGAATCCTGTTTTCCTGCGTAAAATGTGGGAAGCAGCATCGAAATAAGAGAGCAATTGATGATGAAATTACGTGCTTGCCAGAGTTGATCGCAAAATACCGCCATTTTTTTGATAATCTCTCGTTGTAG", "aa": "MYPTSYEMKNSEIRILFSCVKCGKQHRNKRAIDDEITCLPELIAKYRHFFDNLSL", "aa_hexdigest": "1246c9298bb08473bf12fd6713d62cfd", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3D0UWT2", "query_cov": 0.9090909090909091, "subject_cov": 0.9803921568627451, "identity": 0.7, "score": 81.3, "evalue": 2.15e-19, "valid": false, "product": "RNHCP domain-containing protein", "uniref50_id": "UniRef50_A0A3D0UWT2" }, "pscc": { "uniref50_id": "UniRef50_A0A3D0UWT2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3D0UWT2" ], "product": "RNHCP domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_2044", "locus": "GCA003260355_02038" }, { "type": "cds", "sequence": "contig_3", "start": 63596, "stop": 63757, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATTTTTGATCCAGGTCCTCCACTTTTGCTTTATCATAGAGTACCTGTAGAGAGATTTATGAAGGCTGTGGATGAGATGGGAACTTACTATTCATTTATGAAAATTACTGACAAGCATGCGGATCTCGCTCCCTTCTTTCCAGTATCATCGACTCCATAG", "aa": "MIFDPGPPLLLYHRVPVERFMKAVDEMGTYYSFMKITDKHADLAPFFPVSSTP", "aa_hexdigest": "4b150fd6595d5eb27c8d3ed52d157422", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6105.0883, "isoelectric_point": 5.992500877380371 }, "id": "NKOCPLAKPK_2045", "locus": "GCA003260355_02039" }, { "type": "cds", "sequence": "contig_3", "start": 63800, "stop": 63910, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGATATTATTCAAGTAATTAAGGAGAATCAAAGAGAAGGGTGTTTGTTTCTGATTGGTTTGGATAGGTATACTTTACATAATGAGCAACCTTATGAAGATTTACCATGA", "aa": "MDIIQVIKENQREGCLFLIGLDRYTLHNEQPYEDLP", "aa_hexdigest": "d303c47fc4c0032b3e2be365729cca2f", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4304.854700000001, "isoelectric_point": 4.433748435974121 }, "id": "NKOCPLAKPK_2046", "locus": "GCA003260355_02040" }, { "type": "cds", "sequence": "contig_3", "start": 63968, "stop": 64354, "strand": "-", "frame": 3, "gene": null, "product": "Peptidase C39-like domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MPK3", "UniRef:UniRef90_A0A660MPK3" ], "nt": "ATGCTCCGAGATGGAAAACATCCTGAATATTTTTTACCGGAGGCGGTGCCTGGTTTTTGTTCTTTGACGTGTTGTATGGGAGTCTTAAATTATTTTTGGTCGACAAGCTTCAATGGACAGAATACCTGGCAAATGATGAATCTCTTTAAAGAGGGATTGTATACAGGAACTGAAATGTTAGATTTGCCGGAAATTGCCTGTAAATTGGATCAATTGGGATTTAATATTGATTATTATCTCTCCTATAGTGAAGAATTGCATAGAGATTCTTTGGAAAATCCTACGAAATATATCTATGAACGAACACCATCTCAATATCATAAATATATCAAAACAGATGAGCAAGGGTATTATCGAGATTCTGGTACTTCGATTAATAGGCTTTGA", "aa": "MLRDGKHPEYFLPEAVPGFCSLTCCMGVLNYFWSTSFNGQNTWQMMNLFKEGLYTGTEMLDLPEIACKLDQLGFNIDYYLSYSEELHRDSLENPTKYIYERTPSQYHKYIKTDEQGYYRDSGTSINRL", "aa_hexdigest": "926c49583488ef5b0e91412adf8de773", "start_type": "ATG", "rbs_motif": "TTATT", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 63665, "stop": 64354, "observations": { "mutation": [ 63802, 63970 ], "stop": [ 63802, 63970 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A660MPK3", "query_cov": 1.0, "subject_cov": 0.5079365079365079, "identity": 0.992, "score": 276.0, "evalue": 1.84e-92, "gene_start": 1, "gene_end": 128, "reference_start": 1, "reference_end": 128, "reference_sequence": "MLRDGKHPEYFLPEAVPGFCSLTCCMGVLNYFWSTSFNGQNTWQMMNLFKEGLYTGTEMLDLPEIACKLDQLGFNIDYYLSYSEELHRDSLENPTKYIYERIPSQYHKYIKTDEQGYYRDSGTSINRLGDIKFDQKIVDSQSIISHYDVDIIQVIKENQREGCLFLIGLDRYTLHNEQPYEDLPGGHVILASKIEDDHIVIFDPGPPLLLYHKVPIDQFMRSVEEMGTYYAFMKITDKHADLAAFFPVASTP" }, "paralog": false, "identity": 0.9519650655021834, "score": 464.0, "evalue": 3.08e-169, "description": "Internal stop codon at 63802, 63970. Nonsense mutation around 63802, 63970.", "psc": { "uniref90_id": "UniRef90_A0A660MPK3", "product": "Peptidase C39-like domain-containing protein", "uniref50_id": "UniRef50_A0A660MPK3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A660MPK3", "UniRef:UniRef50_A0A660MPK3" ] }, "pscc": { "uniref50_id": "UniRef50_A0A660MPK3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MPK3" ], "product": "Peptidase C39-like domain-containing protein" } }, "id": "NKOCPLAKPK_2047", "locus": "GCA003260355_02041" }, { "type": "cds", "sequence": "contig_3", "start": 64407, "stop": 64622, "strand": "-", "frame": 2, "gene": null, "product": "N-acetyltransferase domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EF53", "UniRef:UniRef90_A0A328EF53" ], "nt": "ATGTTTCATCAGGAAAGAGCTGAAGTTTTGATGGTCGCTCTAGAAGAAGGGCATGCATCTTCTGAATATCAAGCACTTTTGGAGTATGGTCTACAGTTTTTGAAGGAGCAAGGAGCGAAATTTGTGAGCTTTGAAGTTGCCCCGTCAGAGAATGAGTATCTTAGTTTTTTACAGAAAATGGGGGCTAAAGAAGTTTCGGTAAAATATGTTTTATAA", "aa": "MFHQERAEVLMVALEEGHASSEYQALLEYGLQFLKEQGAKFVSFEVAPSENEYLSFLQKMGAKEVSVKYVL", "aa_hexdigest": "0f44d3820a6d90f76d47b44b9b05384c", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 64407, "stop": 64844, "observations": { "mutation": [ 64640 ], "stop": [ 64640 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EF53", "query_cov": 1.0, "subject_cov": 0.4896551724137931, "identity": 1.0, "score": 137.0, "evalue": 2.36e-40, "gene_start": 1, "gene_end": 71, "reference_start": 75, "reference_end": 145, "reference_sequence": "MKIIPATQNHLQFIATLMVKEYQKLNDDLKCPMYQTDYDLFMRVWSKRIGDQSSEFPLFVAEGEKGEFGGFVALMFHQERAEVLMVALEEGHASSEYQALLEYGLQFLKEQGAKFVSFEVAPSENEYLSFLQKMGAKEVSVKYVL" }, "paralog": false, "identity": 0.993103448275862, "score": 286.0, "evalue": 1.66e-101, "description": "Internal stop codon at 64640. Nonsense mutation around 64640.", "psc": { "uniref90_id": "UniRef90_A0A328EF53", "product": "N-acetyltransferase domain-containing protein", "uniref50_id": "UniRef50_A0A328EF53", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EF53", "UniRef:UniRef50_A0A328EF53" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EF53", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EF53" ], "product": "N-acetyltransferase domain-containing protein" } }, "id": "NKOCPLAKPK_2048", "locus": "GCA003260355_02042" }, { "type": "cds", "sequence": "contig_3", "start": 64638, "stop": 64844, "strand": "-", "frame": 2, "gene": null, "product": "N-acetyltransferase domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EF53", "UniRef:UniRef90_A0A328EF53" ], "nt": "ATGAAAATCATTCCAGCGACGCAAAACCACCTTCAATTCATTGCCACTTTGATGGTAAAGGAATATCAAAAGTTAAATGACGATCTTAAATGCCCGATGTATCAGACGGATTATGACTTATTTATGCGTGTTTGGAGTAAGAGAATTGGAGATCAAAGTTCAGAGTTTCCTCTATTTGTTGCAGAGGGAGAGAAGGGAGAGTTTTGA", "aa": "MKIIPATQNHLQFIATLMVKEYQKLNDDLKCPMYQTDYDLFMRVWSKRIGDQSSEFPLFVAEGEKGEF", "aa_hexdigest": "15f81d91210e76ef056cbca8fc2054ee", "start_type": "ATG", "rbs_motif": "TTTTTA", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 64407, "stop": 64844, "observations": { "mutation": [ 64640 ], "stop": [ 64640 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EF53", "query_cov": 1.0, "subject_cov": 0.4689655172413793, "identity": 1.0, "score": 144.0, "evalue": 5.37e-43, "gene_start": 1, "gene_end": 68, "reference_start": 1, "reference_end": 68, "reference_sequence": "MKIIPATQNHLQFIATLMVKEYQKLNDDLKCPMYQTDYDLFMRVWSKRIGDQSSEFPLFVAEGEKGEFGGFVALMFHQERAEVLMVALEEGHASSEYQALLEYGLQFLKEQGAKFVSFEVAPSENEYLSFLQKMGAKEVSVKYVL" }, "paralog": false, "identity": 0.993103448275862, "score": 286.0, "evalue": 1.49e-101, "description": "Internal stop codon at 64640. Nonsense mutation around 64640.", "psc": { "uniref90_id": "UniRef90_A0A328EF53", "product": "N-acetyltransferase domain-containing protein", "uniref50_id": "UniRef50_A0A328EF53", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EF53", "UniRef:UniRef50_A0A328EF53" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EF53", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EF53" ], "product": "N-acetyltransferase domain-containing protein" } }, "id": "NKOCPLAKPK_2049", "locus": "GCA003260355_02043" }, { "type": "cds", "sequence": "contig_3", "start": 64878, "stop": 65234, "strand": "-", "frame": 2, "gene": null, "product": "Peptidase C39-like domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EFM8", "UniRef:UniRef90_A0A328EBU7" ], "nt": "ATGAATGGAAATAATAGCTTTGAACTCTATGATACCTATATTACTCAGAAAATTCTAGAAACTGATGAAATTGAAAAGATCTATAATGCTGATCTTGTAAGCATTGTGGAACACTACCAAAACGAAGATACGCTCTTTATTTTTCCTCTTAATCGATATGTGCTTTATGATCAGCCTCATGAATCTGGTATTAGTGGAGGTCATATCGTATTGTGTAAGGGGTATAAAGATGGAAGGTTTGAAATCTATGATCCAGGTCCTTACCCTAAACCTGACTTTATCCCGAAGCAAAGAGTCTTAAACGCAATGACTGAACTTGATCAGCATTATGACTTTATTGTGGTAAGAAATACGTAA", "aa": "MNGNNSFELYDTYITQKILETDEIEKIYNADLVSIVEHYQNEDTLFIFPLNRYVLYDQPHESGISGGHIVLCKGYKDGRFEIYDPGPYPKPDFIPKQRVLNAMTELDQHYDFIVVRNT", "aa_hexdigest": "19c124f9e00be8c32fc42717ae32c92c", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 64878, "stop": 65474, "observations": { "mutation": [ 65303, 65345 ], "stop": [ 65303, 65345 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EBU7", "query_cov": 1.0, "subject_cov": 0.5959595959595959, "identity": 1.0, "score": 249.0, "evalue": 4.5e-83, "gene_start": 1, "gene_end": 118, "reference_start": 81, "reference_end": 198, "reference_sequence": "MKDTRDVMKIAKMGRYKFGVGLDECEMAYALSRLGFKVIYVSSGSYENDLAYLEDPVGYIAKKYENHPYKEYIKDGVWVDMNGNNSFELYDTYITQKILETDEIEKIYNADLVSIVEHYQNEDTLFIFPLNRYVLYDQPHESGISGGHIVLCKGYKDGRFEIYDPGPYPKPDFIPKQRVLNAMTELDQHYDFIVVRNT" }, "paralog": false, "identity": 0.98989898989899, "score": 407.0, "evalue": 1.45e-147, "description": "Internal stop codon at 65303, 65345. Nonsense mutation around 65303, 65345.", "psc": { "uniref90_id": "UniRef90_A0A328EBU7", "product": "Peptidase C39-like domain-containing protein", "uniref50_id": "UniRef50_A0A328EFM8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EBU7", "UniRef:UniRef50_A0A328EFM8" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EFM8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EFM8" ], "product": "Peptidase C39-like domain-containing protein" } }, "id": "NKOCPLAKPK_2050", "locus": "GCA003260355_02044" }, { "type": "cds", "sequence": "contig_3", "start": 65343, "stop": 65585, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGACTATCCAAAGTATCAAATTAACTTTATGCCAGAGTCGTATTATGGCTACTGTAGTACTGCATGTTTCATGGGGATTTTGAATTATTTCCGATATACTCAGTTTAATATGAAAGATACCCGAGATGTGATGAAAATCGCTAAAATGGGAAGATATAAATTTGGAGTCGGGCTGGATGAATGTGAAATGGCGTATGCCCTTTCAAGGCTCGGTTTTAAGGTAATATACGTATCATCATGA", "aa": "MDYPKYQINFMPESYYGYCSTACFMGILNYFRYTQFNMKDTRDVMKIAKMGRYKFGVGLDECEMAYALSRLGFKVIYVSS", "aa_hexdigest": "cf97a5ca4574324c759c0397418e478f", "start_type": "ATG", "rbs_motif": "TTCTT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9461.958, "isoelectric_point": 8.773583030700681 }, "id": "NKOCPLAKPK_2051", "locus": "GCA003260355_02045" }, { "type": "cds", "sequence": "contig_3", "start": 65596, "stop": 65730, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTGAAATTTATGATCCAGGTCCTTATAAAAAACCTGATTTAATACCGATAGAAAAAGTCATAAAAGCTATGGATGATTTAAACAAGACTTATAGTATCATAGCAGTGTCTGATATCAATTCTACTTCTTAA", "aa": "MFEIYDPGPYKKPDLIPIEKVIKAMDDLNKTYSIIAVSDINSTS", "aa_hexdigest": "72911c0c322bf5ec1e7b4bbad3eaa900", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4974.6977, "isoelectric_point": 4.6018781661987305 }, "id": "NKOCPLAKPK_2052", "locus": "GCA003260355_02046" }, { "type": "cds", "sequence": "contig_3", "start": 65685, "stop": 65807, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTTGTATGATCAAGAGCCAGAAGAATGAATTAGTTGATGACATGTTGTTTTGTGTAAATGATACAAAGATTGAATGTTTGAAATTTATGATCCAGGTCCTTATAAAAAACCTGATTTAA", "aa": "MFCMIKSQKNELVDDMLFCVNDTKIECLKFMIQVLIKNLI", "aa_hexdigest": "d0668c634182588b206396f7ef3f84c2", "start_type": "ATG", "rbs_motif": "TTCTT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4753.817499999998, "isoelectric_point": 5.993182945251465 }, "id": "NKOCPLAKPK_2053", "locus": "GCA003260355_02047" }, { "type": "cds", "sequence": "contig_3", "start": 65776, "stop": 65964, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGGCAACAATACTTTTGATTTTTGCGATACATATATATCTAAGAAAGTACTTGAATGTTCAGAAATAGATAAATTTTATAATATCGAACCTTTGTCTTTCATAAAAAAATATCAGAATGAAAATACTCTTTTTATTCTTTCTGTTAACCGAAATGTTTTGTATGATCAAGAGCCAGAAGAATGA", "aa": "MNGNNTFDFCDTYISKKVLECSEIDKFYNIEPLSFIKKYQNENTLFILSVNRNVLYDQEPEE", "aa_hexdigest": "96dafd234fd3f825b7ff2d0143858fcb", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7406.204500000001, "isoelectric_point": 4.497351264953613 }, "id": "NKOCPLAKPK_2054", "locus": "GCA003260355_02048" }, { "type": "cds", "sequence": "contig_3", "start": 66148, "stop": 66315, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGACTATCCAAAGTATCAAATCAACTTTATGCCAGAGTCCTATTATGGCTACTGTAGTACTGCATGTTTCATGGGGATTTTGAATTATTTCCGATATACGCAATTTAATATGAAGGATACCCGAGATGTGATGAAAATCGCTAAAATGGGAAGATATAAATTTTGA", "aa": "MDYPKYQINFMPESYYGYCSTACFMGILNYFRYTQFNMKDTRDVMKIAKMGRYKF", "aa_hexdigest": "04134a75e7f4cd20b7223d32ef253d95", "start_type": "ATG", "rbs_motif": "TTATT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6771.8605000000025, "isoelectric_point": 9.199332237243652 }, "id": "NKOCPLAKPK_2055", "locus": "GCA003260355_02049" }, { "type": "cds", "sequence": "contig_3", "start": 66682, "stop": 67080, "strand": "-", "frame": 1, "gene": null, "product": "CYTH domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EBM3", "UniRef:UniRef90_A0A328EBM3" ], "nt": "ATGGCTGTAGAGTACGAAACTAAAGTTTTAAATATTGATGTAGAGGCTGTGAAGCAGCAGTTAAAGGAACTTGGAGCAGTGTTTGTTGGGAAAAAAAACTTTCGTAGATATGTATATGATATGAATCCACCCCAAGCAGAAAAACGGATGAGGCTTAGAACAGATGGGAAGATCACAACCCTAACAATAAAACATATTGTTGATAGAAAAGCGATTGACGGAGTACAAGAACGAGAAGTTGGAGTAGAGGATTTTGACCAGATGAACACTCTTCTTGAGCAATTAGATTATCGTGCAAAAAGTTATCAAGAGAATGTACGTGAATCTTTTATTCTGGGAGATTGTAATTTGGAGGTTGATAGTCGACCACTTATTCCTGCATATTTAGAGATTGAATGA", "aa": "MAVEYETKVLNIDVEAVKQQLKELGAVFVGKKNFRRYVYDMNPPQAEKRMRLRTDGKITTLTIKHIVDRKAIDGVQEREVGVEDFDQMNTLLEQLDYRAKSYQENVRESFILGDCNLEVDSRPLIPAYLEIE", "aa_hexdigest": "c6f5131970dbb0ed5247b07803387a74", "start_type": "ATG", "rbs_motif": "TTTTTA", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 66547, "stop": 67080, "observations": { "mutation": [ 66684 ], "stop": [ 66684 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EBM3", "query_cov": 1.0, "subject_cov": 0.7457627118644068, "identity": 1.0, "score": 255.0, "evalue": 2.91e-85, "gene_start": 1, "gene_end": 132, "reference_start": 1, "reference_end": 132, "reference_sequence": "MAVEYETKVLNIDVEAVKQQLKELGAVFVGKKNFRRYVYDMNPPQAEKRMRLRTDGKITTLTIKHIVDRKAIDGVQEREVGVEDFDQMNTLLEQLDYRAKSYQENVRESFILGDCNLEVDSRPLIPAYLEIEGPNKEAVVEVLRKLSVSGEVTSENTTEVYKRYGINIKDYPTLSFS" }, "paralog": false, "identity": 0.9943502824858758, "score": 345.0, "evalue": 2.58e-123, "description": "Internal stop codon at 66684. Nonsense mutation around 66684.", "psc": { "uniref90_id": "UniRef90_A0A328EBM3", "product": "CYTH domain-containing protein", "uniref50_id": "UniRef50_A0A328EBM3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EBM3", "UniRef:UniRef50_A0A328EBM3" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EBM3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EBM3" ], "product": "CYTH domain-containing protein" } }, "id": "NKOCPLAKPK_2056", "locus": "GCA003260355_02050" }, { "type": "cds", "sequence": "contig_3", "start": 67630, "stop": 67851, "strand": "-", "frame": 1, "gene": null, "product": "GIY-YIG domain-containing protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DC02CB4", "UniRef:UniRef100_A0A328EF25", "UniRef:UniRef50_A0A328EF25", "UniRef:UniRef90_A0A328EF25" ], "nt": "ATGGAGTTTGTAGTCTATATCTTAAAAGGGAAAAGGTATTATGTAGGATATACTGGAAATTTGCAGGGAAGGCTAGAGGAACACAAGAGAGGACAAACAAAAACAACTAGAGAAATAGGAGCATGGGAGTTGGTAAAAGTAACCCCTTGCGCTTCTAAAACAGAAGCTATTATTTTAGAAAGAAAAATAAAAAGAAGCGGACATGTAGAGAGGTGGTTGTAG", "aa": "MEFVVYILKGKRYYVGYTGNLQGRLEEHKRGQTKTTREIGAWELVKVTPCASKTEAIILERKIKRSGHVERWL", "aa_hexdigest": "a13fdbb1320610d61009f11401358558", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000DC02CB4", "uniref100_id": "UniRef100_A0A328EF25", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DC02CB4", "UniRef:UniRef100_A0A328EF25" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EF25", "uniref90_id": "UniRef90_A0A328EF25" }, "psc": { "uniref90_id": "UniRef90_A0A328EF25", "product": "GIY-YIG domain-containing protein", "uniref50_id": "UniRef50_A0A328EF25" }, "pscc": { "uniref50_id": "UniRef50_A0A328EF25", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EF25" ], "product": "GIY-YIG domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_2057", "locus": "GCA003260355_02051" }, { "type": "cds", "sequence": "contig_3", "start": 67958, "stop": 68179, "strand": "-", "frame": 3, "gene": null, "product": "GIY-YIG domain-containing protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBFBD42", "UniRef:UniRef100_A0A328EB08", "UniRef:UniRef50_A0A328EF25", "UniRef:UniRef90_A0A328EB08" ], "nt": "ATGAAGTTCGTAGTCTATATCTTAAAAGGGAAAAGGTATTATGTAGGATATACTGGAAATTTGCAGGGAAGGCTAGAGGAACACAAGAGAGGACAAACAAAAACAACTAGAGAAATAGGAGCATGGGAGTTGGTAAAAGTAACCCCTTGCGCTTCTAAAACAGAAGCTATTATTTTAGAAAGAAAAATAAAAAGAAGCGGACATGTAGAGAGGTGGTTGTAG", "aa": "MKFVVYILKGKRYYVGYTGNLQGRLEEHKRGQTKTTREIGAWELVKVTPCASKTEAIILERKIKRSGHVERWL", "aa_hexdigest": "87e1e3d707d493a010c2922924207318", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000DBFBD42", "uniref100_id": "UniRef100_A0A328EB08", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBFBD42", "UniRef:UniRef100_A0A328EB08" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EB08", "uniref90_id": "UniRef90_A0A328EB08" }, "psc": { "uniref90_id": "UniRef90_A0A328EB08", "product": "GIY-YIG domain-containing protein", "uniref50_id": "UniRef50_A0A328EF25" }, "pscc": { "uniref50_id": "UniRef50_A0A328EF25", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EF25" ], "product": "GIY-YIG domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_2058", "locus": "GCA003260355_02052" }, { "type": "tRNA", "sequence": "contig_3", "start": 68234, "stop": 68306, "strand": "-", "gene": "trnK", "product": "tRNA-Lys(ttt)", "amino_acid": "Lys", "anti_codon": "ttt", "score": 72.8, "nt": "GGGGTAGTAGCTCAGTCGGTAGAGCAACAGCCTTTTAAGCTGGTGGTCCTGGGTTCGATCCCCAGCTACCCCA", "db_xrefs": [ "SO:0000265" ], "anti_codon_pos": [ 68272, 68274 ], "id": "NKOCPLAKPK_38", "locus": "GCA003260355_02053" }, { "type": "cds", "sequence": "contig_3", "start": 68398, "stop": 68586, "strand": "-", "frame": 1, "gene": "rpmB", "product": "50S ribosomal protein L28", "db_xrefs": [ "GO:0003735", "GO:0005840", "GO:0006412", "SO:0001217", "UniParc:UPI000DBF685B", "UniRef:UniRef100_A0A660MXK4", "UniRef:UniRef50_A0A660MXK4", "UniRef:UniRef90_A0A660MXK4" ], "nt": "ATGGCTAGAATATGTTATTTTACAGGGAGAAAATCTCAATCTGGACAGACAAGATCTCACTCTATGAGAGCAGGGAAGAGACAGTTTAAGGTAAATCTCATCAATAAATGGATTAAACTTGATGATGGAAGTCAGATTAAGGTAAAGATTAACTCTAGACTTTACAAAAAGTTTAAAGGATTTATGTAG", "aa": "MARICYFTGRKSQSGQTRSHSMRAGKRQFKVNLINKWIKLDDGSQIKVKINSRLYKKFKGFM", "aa_hexdigest": "ea4a45477331b9ca4bcb05fbe6584a58", "start_type": "ATG", "rbs_motif": "TTATT", "ups": { "uniparc_id": "UPI000DBF685B", "uniref100_id": "UniRef100_A0A660MXK4", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBF685B", "UniRef:UniRef100_A0A660MXK4" ] }, "ips": { "uniref100_id": "UniRef100_A0A660MXK4", "uniref90_id": "UniRef90_A0A660MXK4" }, "psc": { "uniref90_id": "UniRef90_A0A660MXK4", "gene": "rpmB", "product": "50S ribosomal protein L28", "uniref50_id": "UniRef50_A0A660MXK4", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_A0A660MXK4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MXK4" ], "product": "Large ribosomal subunit protein bL28" }, "genes": [ "rpmB" ], "id": "NKOCPLAKPK_2059", "locus": "GCA003260355_02054" }, { "type": "cds", "sequence": "contig_3", "start": 68621, "stop": 69223, "strand": "-", "frame": 3, "gene": null, "product": "50S ribosomal protein L25", "db_xrefs": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0008097", "KEGG:K02897", "SO:0001217", "UniParc:UPI000DC03855", "UniRef:UniRef100_A0A328ED16", "UniRef:UniRef50_A0A288DD61", "UniRef:UniRef90_A0A328ED16" ], "nt": "ATGAAACTTACTGTTCAGGTTCGTACTGCAACTGGGAAAAAAGTGAAAAACTTGAGAAAAGAGGGGCAGCTTCCTGCTATCGTATATGGAAAGAGCTTAGAAAATCCTGTGCTCTTGACTTGTCTAAAGAATGATTTTATCAGAGTATATAGAGAAGCTGGATTCTCTACGCCTATTGAACTTACTGGGGCGATAGAGCAGCTTGTGCTTTTGCAAGATATGCAGTTGGATCCGGTATCTGATGAAGTACTTACTATAGATTTCTTGGCAGTAAATAGGAATGAAAAGGTATCTGCGTCTGTTCCTGTAGTGCTCGTTGGTGAATCAAAAGTTGAAAAACTGAATGAAGGAAAGGTTCAGCTTGTAAAAGATATTGTAGAAGTAGAAGCCTTCCCTCAAGACCTTCCTCATAATATTGAGATTGATGTAGCAACGATCGAATCAATAAACGACGTAATCTTTGTAAAGGATTTGAAGGTTTCAAGCAAGGTAAAAGTTCTTGATGATGGAGATCAGCCAGTAGTGACCGTTGTTGAACTTGCTGATGAAGAAGAAGCTTCAGATGAAGCTGCAACACCAGAGGCTACTCCAGCTGCTGCATAG", "aa": "MKLTVQVRTATGKKVKNLRKEGQLPAIVYGKSLENPVLLTCLKNDFIRVYREAGFSTPIELTGAIEQLVLLQDMQLDPVSDEVLTIDFLAVNRNEKVSASVPVVLVGESKVEKLNEGKVQLVKDIVEVEAFPQDLPHNIEIDVATIESINDVIFVKDLKVSSKVKVLDDGDQPVVTVVELADEEEASDEAATPEATPAAA", "aa_hexdigest": "2e7539468f09df760d2ef0ae8fa0ce91", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000DC03855", "uniref100_id": "UniRef100_A0A328ED16", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DC03855", "UniRef:UniRef100_A0A328ED16" ] }, "ips": { "uniref100_id": "UniRef100_A0A328ED16", "uniref90_id": "UniRef90_A0A328ED16" }, "psc": { "uniref90_id": "UniRef90_A0A328ED16", "product": "50S ribosomal protein L25", "uniref50_id": "UniRef50_A0A288DD61", "kegg_orthology_id": "K02897", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0008097" ] }, "genes": [], "id": "NKOCPLAKPK_2060", "locus": "GCA003260355_02055" }, { "type": "cds", "sequence": "contig_3", "start": 69251, "stop": 69631, "strand": "-", "frame": 3, "gene": null, "product": "50S ribosomal protein L19", "db_xrefs": [ "KEGG:K02884", "SO:0001217", "UniParc:UPI000DC0232E", "UniRef:UniRef100_A0A660MZM1", "UniRef:UniRef50_A0A288DNP6", "UniRef:UniRef90_A0A2W4IMY0" ], "nt": "ATGAATCACGAAATGATAAAGTATCTTTCTGATGCGAATGGATATAAAATCTTGCCTGTAAAAACAGGAATGCAAGTTGAGCTTCACGAAAGAGTGGGAGAAGGTTCTACAGAGAGAATCTGGAAGTTTAAGGGCTTGATCATCAAGGTGAGAAAACCAGGATCAGCTGATGGAAGCTTTACAATCAGAGGACTTGCTGCAGGGCAAACAATTGAAAAGGTTTATCCTCTTTCTTTTCCTAAGTTTGAGAAAGTTATTCTTATTGATGAATACAAGGTAAGAAAGTCTAAGCTCTACTATATTAGAGAAAAAGTTGGAAAGGCAGCGAGAATGAAGAGTAAGATTGCTGCTGAGAGGAGAGAATCAGATTTGTTGAAATAA", "aa": "MNHEMIKYLSDANGYKILPVKTGMQVELHERVGEGSTERIWKFKGLIIKVRKPGSADGSFTIRGLAAGQTIEKVYPLSFPKFEKVILIDEYKVRKSKLYYIREKVGKAARMKSKIAAERRESDLLK", "aa_hexdigest": "0634ecee07c9be46a93b813cdb8f18d0", "start_type": "ATG", "rbs_motif": "TTCTT", "ups": { "uniparc_id": "UPI000DC0232E", "uniref100_id": "UniRef100_A0A660MZM1", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DC0232E", "UniRef:UniRef100_A0A660MZM1" ] }, "ips": { "uniref100_id": "UniRef100_A0A660MZM1", "uniref90_id": "UniRef90_A0A2W4IMY0" }, "psc": { "uniref90_id": "UniRef90_A0A2W4IMY0", "product": "50S ribosomal protein L19", "uniref50_id": "UniRef50_A0A288DNP6", "kegg_orthology_id": "K02884" }, "pscc": { "uniref50_id": "UniRef50_A0A288DNP6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A288DNP6" ], "product": "50S ribosomal protein L19" }, "genes": [], "id": "NKOCPLAKPK_2061", "locus": "GCA003260355_02056" }, { "type": "cds", "sequence": "contig_3", "start": 69657, "stop": 69803, "strand": "-", "frame": 2, "gene": null, "product": "50S ribosomal protein L29", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7W4E2S3", "UniRef:UniRef90_A0A7W4E2S3" ], "nt": "ATGGCAAGATTAAAGACAAATGCAAAGAGAAAGCTCAATAAAGCACTTACAAGAGTTGAAAAAGTGGTGATTATGGATGTAAGAAGAATGCTTGAGCAACAGAGAAAATGGAAAGCTAAAGTAATGAATAGCAAAGGAAGCAAGTAG", "aa": "MARLKTNAKRKLNKALTRVEKVVIMDVRRMLEQQRKWKAKVMNSKGSK", "aa_hexdigest": "725bfee7062f861e99342898c6086813", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A7W4E2S3", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.9790000000000001, "score": 84.7, "evalue": 6.29e-21, "valid": true, "product": "50S ribosomal protein L29", "uniref50_id": "UniRef50_A0A7W4E2S3" }, "pscc": { "uniref50_id": "UniRef50_A0A7W4E2S3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7W4E2S3" ], "product": "50S ribosomal protein L29" }, "genes": [], "id": "NKOCPLAKPK_2062", "locus": "GCA003260355_02057" }, { "type": "cds", "sequence": "contig_3", "start": 69808, "stop": 70029, "strand": "-", "frame": 1, "gene": "recX", "product": "Regulatory protein RecX", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4KUC3", "UniRef:UniRef90_A0A660N0B2" ], "nt": "TTGAATTTGCACAGACCAAAGACGGGTTGTAAAAATGAAAAAGCAGTAGAAAGCTATGTTACCCTTTTGAAGAGAGAAGAAAAAGAGGGGTTGACTGCTTGGATCAAGAATGCTAAAGTAAAGGCTGAAGCGAAACTTAAGAAATATGGAATTACTCAGCAGAAGATCAAAGAAGTTCTTGAATCAAAAGGACTCGCTCATTTATCATCTAAATTATCATAA", "aa": "MNLHRPKTGCKNEKAVESYVTLLKREEKEGLTAWIKNAKVKAEAKLKKYGITQQKIKEVLESKGLAHLSSKLS", "aa_hexdigest": "8d635e18ae1619e4eb4bb0ca5d979cac", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A660N0B2", "query_cov": 1.0, "subject_cov": 0.8295454545454546, "identity": 0.986, "score": 136.0, "evalue": 1.31e-40, "valid": true, "product": "Regulatory protein RecX", "uniref50_id": "UniRef50_A0A2W4KUC3" }, "pscc": { "uniref50_id": "UniRef50_A0A2W4KUC3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4KUC3" ], "product": "Regulatory protein RecX" }, "genes": [ "recX" ], "id": "NKOCPLAKPK_2063", "locus": "GCA003260355_02058" }, { "type": "cds", "sequence": "contig_3", "start": 70134, "stop": 70589, "strand": "-", "frame": 2, "gene": "rpsG", "product": "30S ribosomal protein S7", "db_xrefs": [ "GO:0003735", "GO:0006412", "GO:0015935", "KEGG:K02992", "SO:0001217", "UniParc:UPI000DBFFDDD", "UniRef:UniRef100_A0A660MZM6", "UniRef:UniRef50_A0A837IKM9", "UniRef:UniRef90_A0A7W4EC62" ], "nt": "ATGGCAGTGATTAAAAATAAGACTTTGGACACCTTTGATTACGAAGAAAAGTTGATCAACTTCATCATGAAGCATGGGAAGAAAAGTGTTGCAAAGAAAATTTATGCGGACACCCTCAGAGATATTAAAGCAAGCGGACATATGAACCCTACTGTAGTAGTGAGAACTGCTATTGAAAATGCTTCTCCTGTGACGATGATCAAGTCAAAGAGAATTGGAGGTGCAGTATATCAGGTTCCAGTAGAGGTAAAAGCTGACAAGAGATTCTTTTTTGCTACAAAATGGATTCTTGATGCTGCGAGAGGAAAGAGTGGAAAGCCGATGTACAAGAGACTTGCTGAGGAAATCCTCGCTGCATATTCTAATCAAGGAGCTGCGGTAAAGAAGAAGGAAGAAGCTCACAAGATGGCTGAAGCTAATAAGGCATTCGCTTATATGGCTAAATATATTAACTAG", "aa": "MAVIKNKTLDTFDYEEKLINFIMKHGKKSVAKKIYADTLRDIKASGHMNPTVVVRTAIENASPVTMIKSKRIGGAVYQVPVEVKADKRFFFATKWILDAARGKSGKPMYKRLAEEILAAYSNQGAAVKKKEEAHKMAEANKAFAYMAKYIN", "aa_hexdigest": "bec2d1e6ed1c03378a13b22fd1830fc5", "start_type": "ATG", "rbs_motif": "TTTTT", "ups": { "uniparc_id": "UPI000DBFFDDD", "uniref100_id": "UniRef100_A0A660MZM6", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBFFDDD", "UniRef:UniRef100_A0A660MZM6" ] }, "ips": { "uniref100_id": "UniRef100_A0A660MZM6", "uniref90_id": "UniRef90_A0A7W4EC62" }, "psc": { "uniref90_id": "UniRef90_A0A7W4EC62", "gene": "rpsG", "product": "30S ribosomal protein S7", "uniref50_id": "UniRef50_A0A837IKM9", "kegg_orthology_id": "K02992", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015935" ] }, "pscc": { "uniref50_id": "UniRef50_A0A837IKM9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A837IKM9" ], "product": "Small ribosomal subunit protein uS7" }, "genes": [ "rpsG" ], "id": "NKOCPLAKPK_2064", "locus": "GCA003260355_02059" }, { "type": "cds", "sequence": "contig_3", "start": 70617, "stop": 71039, "strand": "-", "frame": 2, "gene": "rpsL", "product": "30S ribosomal protein S12", "db_xrefs": [ "GO:0003735", "GO:0006412", "GO:0015935", "KEGG:K02950", "SO:0001217", "UniParc:UPI000DBF4173", "UniRef:UniRef100_A0A660MY78", "UniRef:UniRef50_P92532", "UniRef:UniRef90_A0A660MY78" ], "nt": "ATGCCAACGATTAATCAACTTATTAAAAAAGGAAGAGTAAAGAAAACCTTTAAGTCAAAGGCACCTGTCCTTCAGTTTGGAGTAAACAAGCTTCAAGGAAACAAAAAGGTTGCACAGCCTGCTCCATTCAAGAGAGGGGTATGTCTTAAGGTATCAGTAATGGGACCAAAGAAACCAAATTCAGCTCAGAGAAAGTTTGCAAAGGTAAGGCTTTCTAATGGTGCAGAAATATTGGCATATATTCCAGGAGAGGCTCACTCACTTCAAGAGCATAGTGTTGTGCTTGTAAGAGGAGGAAGGGTGAAGGATCTGCCAGGTGTGAGATACCATATCGTGAGAGGAAAATACGATGCAAATGCAGTAGCAAATAGAAAGCAGAGTAGATCTCGCTATGGAGCAAAGAGACCTAAGGCTGGTAAATAA", "aa": "MPTINQLIKKGRVKKTFKSKAPVLQFGVNKLQGNKKVAQPAPFKRGVCLKVSVMGPKKPNSAQRKFAKVRLSNGAEILAYIPGEAHSLQEHSVVLVRGGRVKDLPGVRYHIVRGKYDANAVANRKQSRSRYGAKRPKAGK", "aa_hexdigest": "a698a617542c0f534f8f8f6ec0adc06a", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000DBF4173", "uniref100_id": "UniRef100_A0A660MY78", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBF4173", "UniRef:UniRef100_A0A660MY78" ] }, "ips": { "uniref100_id": "UniRef100_A0A660MY78", "uniref90_id": "UniRef90_A0A660MY78" }, "psc": { "uniref90_id": "UniRef90_A0A660MY78", "gene": "rpsL", "product": "30S ribosomal protein S12", "uniref50_id": "UniRef50_P92532", "kegg_orthology_id": "K02950", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015935" ] }, "genes": [ "rpsL" ], "id": "NKOCPLAKPK_2065", "locus": "GCA003260355_02060" }, { "type": "cds", "sequence": "contig_3", "start": 71757, "stop": 71972, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAGTGCCGTTCGGAAGAAAAAATATGAAGGCGACTTTTGTATCCAGCTCTACAACAGTGCGGTGATTACCGAGGAGATGAAGGCAAAGAGCGGAAACTGTGATATTATTACCTTCGCTGCTAGTGGGACGACCCGTTTAAAGACGGGACTTGCCGCCCTAGACAGTGCTCAGCCTTCAAATCTTGAGGTCGAGTATGTGAACTTAGAAGACTAG", "aa": "MSAVRKKKYEGDFCIQLYNSAVITEEMKAKSGNCDIITFAASGTTRLKTGLAALDSAQPSNLEVEYVNLED", "aa_hexdigest": "3e643e9fb71fe923c4a9f60b5b4f44ed", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7749.694799999999, "isoelectric_point": 4.993100929260252 }, "id": "NKOCPLAKPK_2066", "locus": "GCA003260355_02061" }, { "type": "cds", "sequence": "contig_3", "start": 71991, "stop": 72479, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EG97", "UniRef:UniRef90_A0A328EBN4" ], "nt": "GTGATGACGGTCATCGTTTGCATCATCACCGCTTGCCTCCAGAAAAAATCCAAAATTCTGAGAGGAATCAGGCGATTTTTCTTGGGAATCACGATGCTTGTTGGAGCTTTTTTCGTTTTAGTCGGAGTGCTTCACGCGATCGGGGTTAAACAAGATTTAAAAGATGGGGGAAGATTCCTTTTCGGAGCAAGAGACAGTTTTTTCATTCAACTCATCGGATTAGACAACCAAAATGGTCCTGAAGCCCACGCCCTAAAGCCAGCTCCCTTCCAAGATGACGAGGGAGACGAGAAAACCTTTACGCTTGCTCCTGGAATGAAGCTAGGAATAGATGCTACGCTAGGAAATGATGTTAATCTTGATGTCGCTCCAGCACAAAAGGCAGGAATCAGACTTACCTTCACAAATGCAAAGTTCGTCTTGAGAGGAATGGAGCTTCTCAATCCAAATCCAAGAGAAATCGCTGCCTATGATAAGATCGATTATTGA", "aa": "MMTVIVCIITACLQKKSKILRGIRRFFLGITMLVGAFFVLVGVLHAIGVKQDLKDGGRFLFGARDSFFIQLIGLDNQNGPEAHALKPAPFQDDEGDEKTFTLAPGMKLGIDATLGNDVNLDVAPAQKAGIRLTFTNAKFVLRGMELLNPNPREIAAYDKIDY", "aa_hexdigest": "47507d0c77e06ed349664601befe883b", "start_type": "GTG", "rbs_motif": "TTATT", "genes": [], "truncated": "both", "pseudogene": { "start": 71757, "stop": 72545, "observations": { "mutation": [ 71993, 72488 ], "stop": [ 71993, 72488 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EBN4", "query_cov": 0.9938271604938271, "subject_cov": 0.6145038167938931, "identity": 1.0, "score": 321.0, "evalue": 1.15e-109, "gene_start": 2, "gene_end": 162, "reference_start": 24, "reference_end": 184, "reference_sequence": "MGVQSAGEMVISLLLELFAGAIVMTVIVCIITACLQKKSKILRGIRRFFLGITMLVGAFFVLVGVLHAIGVKQDLKDGGRFLFGARDSFFIQLIGLDNQNGPEAHALKPAPFQDDEGDEKTFTLAPGMKLGIDATLGNDVNLDVAPAQKAGIRLTFTNAKFVLRGMELLNPNPREIAAYDKIDYGFLAQDYVSAVRKKKYEGDFCIQLYNSAVITEEMKAKSGNCDIITFAASGTTRLKTGLAALDSAQPSNLEVEYVNLED" }, "paralog": false, "identity": 0.9923664122137404, "score": 506.0, "evalue": 4.18e-185, "description": "Internal stop codon at 71993, 72488. Nonsense mutation around 71993, 72488.", "psc": { "uniref90_id": "UniRef90_A0A328EBN4", "uniref50_id": "UniRef50_A0A328EG97", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EBN4", "UniRef:UniRef50_A0A328EG97" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EG97", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EG97" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 17754.61949999999, "isoelectric_point": 8.58062915802002 }, "id": "NKOCPLAKPK_2067", "locus": "GCA003260355_02062" }, { "type": "cds", "sequence": "contig_3", "start": 72626, "stop": 72832, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EF34", "UniRef:UniRef90_A0A328EF34" ], "nt": "TTGCCGAGCTGGAGTCTACTTTTGGAATTTACTCAAATCGTCGTTTTGGGGCAGGCGACGAAAGGCTTTTCACTTCTCATGCTGCGGCAGAGAAGGGGAATTCCTTTGCGGATCGTTGCGGGCGAATGGAATAAAGGCAAGCTCCTCCAGATCAAAGCCCAGTGGTTTTATCGCTATCGTAATGCTGAGGTTTGCAAGGCTCGCTAG", "aa": "MPSWSLLLEFTQIVVLGQATKGFSLLMLRQRRGIPLRIVAGEWNKGKLLQIKAQWFYRYRNAEVCKAR", "aa_hexdigest": "dca9d335a7ddde20681eed508ade5f85", "start_type": "TTG", "rbs_motif": "TTTTT", "psc": { "uniref90_id": "UniRef90_A0A328EF34", "query_cov": 1.0, "subject_cov": 0.8, "identity": 0.985, "score": 138.0, "evalue": 1.68e-41, "valid": true, "uniref50_id": "UniRef50_A0A328EF34" }, "pscc": { "uniref50_id": "UniRef50_A0A328EF34", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EF34" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7979.448799999997, "isoelectric_point": 11.003737831115721 }, "id": "NKOCPLAKPK_2068", "locus": "GCA003260355_02063" }, { "type": "cds", "sequence": "contig_3", "start": 72975, "stop": 73391, "strand": "-", "frame": 2, "gene": null, "product": "Signal peptidase I", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EBT5", "UniRef:UniRef90_A0A328EBT5" ], "nt": "ATGGATCTAACAGGAATTATGGCTCAAAACCCAGAAATGGCATCTCTTGGAGCAGGAGCTATAGGAGGTGCACTAGCAGCAATAGGAACCGTTTTCATTATTTTCACAGTGTTGATTGGTGCGCTTATGATCGTTTCTCGATGGAGAATCTTCGAAAAGGCGGAACTCCCAGGACGAGGAGCATTGATCCCGATCTACAACATTTACTTGATGTTCAAGCTTGCGGGAAAACCAGAATGGACACGGCGACTCCTTCTTCCTCCGGTGGCTGGAGTCTTAGCAATCGTTGCTCAGTTCAAAATCTCCAAGAAGTTCGGGAAAACTGATATGTTTGCTGTGGGGCTTTGGTTTCTACCATTTATTTTTTGTCCAATCCTTGCTTTCTCAGAAGAAGTAAAATACAATCCAGAGGCATAA", "aa": "MDLTGIMAQNPEMASLGAGAIGGALAAIGTVFIIFTVLIGALMIVSRWRIFEKAELPGRGALIPIYNIYLMFKLAGKPEWTRRLLLPPVAGVLAIVAQFKISKKFGKTDMFAVGLWFLPFIFCPILAFSEEVKYNPEA", "aa_hexdigest": "fc9cca9f378d39b979fe0c2850254b70", "start_type": "ATG", "rbs_motif": "TTTTTA", "psc": { "uniref90_id": "UniRef90_A0A328EBT5", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.986, "score": 270.0, "evalue": 1.6e-91, "valid": true, "product": "Signal peptidase I", "uniref50_id": "UniRef50_A0A328EBT5" }, "genes": [], "id": "NKOCPLAKPK_2069", "locus": "GCA003260355_02064" }, { "type": "cds", "sequence": "contig_3", "start": 73545, "stop": 73877, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGACAATCATCGCAGTAGGAACTTCTCTCCCAGAGCTGGCAACTTCTATCCTCGCCGCTTATAGAAAAAACGCAGATATCGCACTCGGAAATGTTATTGGTTCAAACGTTTTCAATATTTTATTTATCCTTGGGGTGAGCGCAGTAATCGCTCCAATCCCTGCTTTGCCGGGCACCAATAATGACCTTATTCTCTTGATTGTTGCCACGCTCCTCATTTTTATCTTTGGAGCTACCCGAGGTGCAAGGAAGGGACAAATAGGTAGAGTGGCAGGCTCAGCAATGCTCCTCTTGTATGTTATCGCTATGGTAAGCCGTTTTTTCCTTCCCTAG", "aa": "MTIIAVGTSLPELATSILAAYRKNADIALGNVIGSNVFNILFILGVSAVIAPIPALPGTNNDLILLIVATLLIFIFGATRGARKGQIGRVAGSAMLLLYVIAMVSRFFLP", "aa_hexdigest": "b461def01658c1575d3a0fa913cc6610", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11481.708499999986, "isoelectric_point": 10.407856559753416 }, "id": "NKOCPLAKPK_2070", "locus": "GCA003260355_02065" }, { "type": "cds", "sequence": "contig_3", "start": 73899, "stop": 74450, "strand": "-", "frame": 2, "gene": null, "product": "Sodium/calcium exchanger membrane region domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EB15", "UniRef:UniRef90_A0A328EB15" ], "nt": "ATGCTTGTTGAGGGAGCTTCTTCCTTGGCAAAAAAATGCGGTATCTCTGATATGGTTATCGGATTGACGATCGTTGCATTCGGAACCTCAGCTCCAGAGCTGGTGGTTAATGTGACTTCCAGCCTTCAAGGAGCGACAGGAATCACCATCGGAAATGTCTTGGGAAGTAATATCGCTAATATCCTGCTCATCCTCGGAGCCGCTGCAGTCATCACCCCGATCACTGTCCAAAAGAAATTTCTTTCTAGGGAAGTGGTGATTTGCTTAGCTGCTACGCTGGTCTTTGCGATCTTTATCAATGACGCTCGGATCGACGGAGCAGCTACTTCACGAGTCTCCAGGAGTGAAGGAATCATCTTGATCCTACTCTTGCTGTTCTTCGTGTATTACCTTTTCGCGACAGCCAAAAGAGAAAATCCTGACACAGAAAGCACAATCGCCATTTCCCCCTTGCGAAAATCCTTACTTTGGATCGGACTCGGGTTGGTTGGACTCCTTTTTGGTGGAGAACGAATCGTTAACGGAGCAATTGCTATCGCTACGAGCTTCTGA", "aa": "MLVEGASSLAKKCGISDMVIGLTIVAFGTSAPELVVNVTSSLQGATGITIGNVLGSNIANILLILGAAAVITPITVQKKFLSREVVICLAATLVFAIFINDARIDGAATSRVSRSEGIILILLLLFFVYYLFATAKRENPDTESTIAISPLRKSLLWIGLGLVGLLFGGERIVNGAIAIATSF", "aa_hexdigest": "c9f45e06ca760ffab80ed09a73284abf", "start_type": "ATG", "rbs_motif": "TTCTT", "genes": [], "truncated": "both", "pseudogene": { "start": 73596, "stop": 74513, "observations": { "mutation": [ 73901, 74504 ], "stop": [ 73901, 74504 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EB15", "query_cov": 1.0, "subject_cov": 0.5683229813664596, "identity": 0.9670000000000001, "score": 318.0, "evalue": 4.77e-107, "gene_start": 1, "gene_end": 183, "reference_start": 22, "reference_end": 204, "reference_sequence": "MSRGIGLLFILGFVFLIKGADMLVEGASSLAKKCGISDMVIGLTIVAFGTSAPELVVNLTSSLQGATGITIGNVLGSNIANILLILGAAAVITPITVQKKFLSREVVICLAATLVFAIFINDARIDGAATSRVSRSEGIILILLLLFFVYYLFATAKRENTDTESTIAIFPLRKSLLWIGLGLVGLLFGGERIVNGAVAIATSFGLSERLIGLTIIAVGTSLPELATSILAAYRKNADIALGNVIGSNVFNILFILGVSAVIAPIPALPGTNNDLILLIVATLLIFIFGATRGARKGQIGRVAGSAMLLLYVVAMVSRFFLP" }, "paralog": false, "identity": 0.9737704918032787, "score": 540.0, "evalue": 3.41e-197, "description": "Internal stop codon at 73901, 74504. Nonsense mutation around 73901, 74504.", "psc": { "uniref90_id": "UniRef90_A0A328EB15", "product": "Sodium/calcium exchanger membrane region domain-containing protein", "uniref50_id": "UniRef50_A0A328EB15", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EB15", "UniRef:UniRef50_A0A328EB15" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EB15", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EB15" ], "product": "Sodium/calcium exchanger membrane region domain-containing protein" } }, "id": "NKOCPLAKPK_2071", "locus": "GCA003260355_02066" }, { "type": "cds", "sequence": "contig_3", "start": 74550, "stop": 74984, "strand": "-", "frame": 2, "gene": null, "product": "L-threonylcarbamoyladenylate synthase", "db_xrefs": [ "EC:2.7.7.87", "GO:0003725", "GO:0006400", "GO:0061710", "SO:0001217", "UniRef:UniRef50_A0A2W4KVQ3", "UniRef:UniRef90_A0A328EB10" ], "nt": "TTGAGACCGGGTTTCATTACCAAAGAGGATCTTGAAGCTTTTTTTGAGGGGCAACCAGTTTCCGTGGAATATACCAGCAAAGACAAAAACCTTTCCCCAGGAACCAGATTTAGACACTATAGTATCCCCGGTAAAATCACCATCATTGATCACAAAACCGAGCTTCCTACATCTTTTGATGTGCCGACGGCAGTCATTGCCACGACAGAGCGATTTGAGCGTGAAAACTTCCACTCTCTCGATCCTCATACTCCATTCCTCACAAAACTCCAACGAGGAAGTGAATCCAACCTCGCAAGCTGTGCACATACCCTTTTCCAGCTCTATCACGACTGCGAAAAAGCAGGAATACAAACGGTCTTTATTCAGAGATTGACAGAAAATGGGCTAGGATTCGCGATTATGAATAGAGTCAAAAGATCTGCTGAAAATTAA", "aa": "MRPGFITKEDLEAFFEGQPVSVEYTSKDKNLSPGTRFRHYSIPGKITIIDHKTELPTSFDVPTAVIATTERFERENFHSLDPHTPFLTKLQRGSESNLASCAHTLFQLYHDCEKAGIQTVFIQRLTENGLGFAIMNRVKRSAEN", "aa_hexdigest": "dbd8ae426b8679c1994aea6680918bf4", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 74550, "stop": 75284, "observations": { "mutation": [ 74990 ], "stop": [ 74990 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EB10", "query_cov": 1.0, "subject_cov": 0.42729970326409494, "identity": 0.986, "score": 293.0, "evalue": 1.18e-97, "gene_start": 1, "gene_end": 144, "reference_start": 194, "reference_end": 337, "reference_sequence": "MQTLISKPSPELYQQAKNLIQSGELVAFPTETIYGLGANALDSDAVQKIFTVKGRPSSNPLIVHIGEKSQIADLAIVENSFQQQIIDKLFPGPVTLLLKKKPGIPEVVTQNPFVGIRMPSNQIANDFLKAVALPIAAPSANISGKPSPTSAPMVMDNVGGKIPLIIDGGDSNYGIESTVIKVDEENNQGKIGILRPGFITKEDLEAFFEGQPVSVEYTSKDKNLSPGTRFRHYSIPGKITIIDHKTELPTSFDAPTAVIATTERFERENFHSLDPHTPFLTKLQRGSESNLASCAHTLFQLYHDCEKAGIQTVFIQRLTENGLGFAIMNRVKRSAEN" }, "paralog": false, "identity": 0.9877049180327869, "score": 482.0, "evalue": 1.24e-174, "description": "Internal stop codon at 74990. Nonsense mutation around 74990.", "psc": { "uniref90_id": "UniRef90_A0A328EB10", "product": "L-threonylcarbamoyladenylate synthase", "ec_ids": [ "2.7.7.87" ], "uniref50_id": "UniRef50_A0A2W4KVQ3", "go_ids": [ "GO:0003725", "GO:0006400", "GO:0061710" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EB10", "EC:2.7.7.87", "UniRef:UniRef50_A0A2W4KVQ3", "GO:0003725", "GO:0006400", "GO:0061710" ] }, "pscc": { "uniref50_id": "UniRef50_A0A2W4KVQ3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4KVQ3" ], "product": "Threonylcarbamoyl-AMP synthase" } }, "id": "NKOCPLAKPK_2072", "locus": "GCA003260355_02067" }, { "type": "cds", "sequence": "contig_3", "start": 74988, "stop": 75428, "strand": "-", "frame": 2, "gene": null, "product": "L-threonylcarbamoyladenylate synthase", "db_xrefs": [ "EC:2.7.7.87", "GO:0003725", "GO:0006400", "GO:0061710", "SO:0001217", "UniRef:UniRef50_A0A2W4KVQ3", "UniRef:UniRef90_A0A328EB10" ], "nt": "GTGCAGAAGATCTTTACAGTAAAAGGCAGACCGAGCAGCAATCCTCTGATCGTCCATATTGGCGAGAAAAGCCAGATCGCAGACCTCGCAATCGTGGAAAATTCCTTCCAGCAGCAAATTATTGACAAGCTTTTCCCTGGACCAGTTACCCTCCTGCTCAAAAAAAAACCAGGAATCCCCGAAGTGGTGACTCAAAATCCCTTCGTAGGGATCAGAATGCCGTCCAACCAAATCGCCAACAACTTTCTCAAAGCGGTAGCATTGCCAATCGCAGCTCCGAGTGCAAACATTTCCGGCAAACCTAGCCCGACTTCTGCCCCAATGGTGATGGACAACGTGGGAGGCAAAATTCCCCTCATTATTGACGGTGGAGACTCAAATTACGGGATAGAGTCGACAGTTATCAAGGTGGACGAAGAAAATAATCAGGGAAAAATCTGA", "aa": "MQKIFTVKGRPSSNPLIVHIGEKSQIADLAIVENSFQQQIIDKLFPGPVTLLLKKKPGIPEVVTQNPFVGIRMPSNQIANNFLKAVALPIAAPSANISGKPSPTSAPMVMDNVGGKIPLIIDGGDSNYGIESTVIKVDEENNQGKI", "aa_hexdigest": "5dd31ece2da707eac4f97562fe0f3ef2", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 74685, "stop": 75563, "observations": { "mutation": [ 74990, 75497 ], "stop": [ 74990, 75497 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EB10", "query_cov": 1.0, "subject_cov": 0.4332344213649852, "identity": 0.986, "score": 279.0, "evalue": 3.74e-92, "gene_start": 1, "gene_end": 146, "reference_start": 46, "reference_end": 191, "reference_sequence": "MQTLISKPSPELYQQAKNLIQSGELVAFPTETIYGLGANALDSDAVQKIFTVKGRPSSNPLIVHIGEKSQIADLAIVENSFQQQIIDKLFPGPVTLLLKKKPGIPEVVTQNPFVGIRMPSNQIANDFLKAVALPIAAPSANISGKPSPTSAPMVMDNVGGKIPLIIDGGDSNYGIESTVIKVDEENNQGKIGILRPGFITKEDLEAFFEGQPVSVEYTSKDKNLSPGTRFRHYSIPGKITIIDHKTELPTSFDAPTAVIATTERFERENFHSLDPHTPFLTKLQRGSESNLASCAHTLFQLYHDCEKAGIQTVFIQRLTENGLGFAIMNRVKRSAEN" }, "paralog": false, "identity": 0.9863013698630136, "score": 566.0, "evalue": 6.15e-208, "description": "Internal stop codon at 74990, 75497. Nonsense mutation around 74990, 75497.", "psc": { "uniref90_id": "UniRef90_A0A328EB10", "product": "L-threonylcarbamoyladenylate synthase", "ec_ids": [ "2.7.7.87" ], "uniref50_id": "UniRef50_A0A2W4KVQ3", "go_ids": [ "GO:0003725", "GO:0006400", "GO:0061710" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EB10", "EC:2.7.7.87", "UniRef:UniRef50_A0A2W4KVQ3", "GO:0003725", "GO:0006400", "GO:0061710" ] }, "pscc": { "uniref50_id": "UniRef50_A0A2W4KVQ3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4KVQ3" ], "product": "Threonylcarbamoyl-AMP synthase" } }, "id": "NKOCPLAKPK_2073", "locus": "GCA003260355_02068" }, { "type": "tRNA", "sequence": "contig_3", "start": 75673, "stop": 75749, "strand": "-", "gene": "trnH", "product": "tRNA-His(gtg)", "amino_acid": "His", "anti_codon": "gtg", "score": 76.5, "nt": "GCGATTGTAGCTCAGCTGGTTAGAGCATCGGATTGTGGTCCCGAGGGTCGCGGGTTCGATTCCCGTCAATCGCCCCA", "db_xrefs": [ "SO:0000262" ], "anti_codon_pos": [ 75713, 75715 ], "id": "NKOCPLAKPK_37", "locus": "GCA003260355_02069" }, { "type": "cds", "sequence": "contig_3", "start": 76409, "stop": 76675, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCAGCCTGAAGGAAAGAAAGCTAAGGAAACTGAGGCGCAAACTTTTAGAGATCGTTCACAGTCCACGTCTCGCTCTTCGGATGCTGAACAAAAGTCGGCTCAGGGCGTTAGCACTGTGGAAACAACCAACCCAGATACTCAAGAAAGCATTAAAGTTGCCGCGGCTCAAGATACTGCAGAGTCGCTTAGCGAAGATGCCAGAGCAAAAAAGGCGCTTGACATCTTGCAGCAGCTTTCTAGGGAAGTAAAGCAAGATGAAGGCTGA", "aa": "MQPEGKKAKETEAQTFRDRSQSTSRSSDAEQKSAQGVSTVETTNPDTQESIKVAAAQDTAESLSEDARAKKALDILQQLSREVKQDEG", "aa_hexdigest": "45276ea5fc5a8e517002eb8a68e0b127", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9584.230699999996, "isoelectric_point": 4.827358436584471 }, "id": "NKOCPLAKPK_2074", "locus": "GCA003260355_02070" }, { "type": "cds", "sequence": "contig_3", "start": 76787, "stop": 77230, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCATGATAAGGCAACTCGTAAAAGGGTGATAAAATCCTTGAAAAAAGAAATTAAAGCTGAAAAGAAAGCCAAATCTGATATCAGAAAGATTGCGAAGCTTGAAGAACAGCTTGCCTCAGTAAAAAATGACAACAACTATTATTATTCAAAGGCGAGCCTTCTAGAGCAGATCAATGCTGCTTTGGATCAGGGAATTTATATCAATACACCGATTGCTTTTCTCAAGCAGACTCCTCTTATCGTAGATATTTATAAAAATAGGAAGAAGATCAAGGGAAATGCCAAGACGGAGATTTCTTCTCAAGGGAAAATGGTCGATGTCTATAATCAAGCAGTAATCACAGGTCAAGTTCATGAGGTAGGAAACTATGTTCAGAGCTGTGGTGGCTACGCTAATGTTGCAGAACAGTGGCTTCATGAAAATACGAAAATGTCACATTGA", "aa": "MHDKATRKRVIKSLKKEIKAEKKAKSDIRKIAKLEEQLASVKNDNNYYYSKASLLEQINAALDQGIYINTPIAFLKQTPLIVDIYKNRKKIKGNAKTEISSQGKMVDVYNQAVITGQVHEVGNYVQSCGGYANVAEQWLHENTKMSH", "aa_hexdigest": "666554b1cfdbb3c60178b3d9b846c49d", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16667.974099999992, "isoelectric_point": 9.595039176940915 }, "id": "NKOCPLAKPK_2075", "locus": "GCA003260355_02071" }, { "type": "cds", "sequence": "contig_3", "start": 77486, "stop": 78070, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTCAGTCTTCAACTGTTGAAAAAATGGCAATTGGACAGAATATGTTGCAATTTGTGCTCTTGGGAGTGCCTTTTAGCACGCTGGCACAGTGCTGCTCTCCAGCAACGGGGATAGTATCACGTATTGATAGTAGTAAGCTCAAAACCTACTATCTCGGAGAAATAGAAAAAGCGAAAGCAAATCGTGACTCAAAATCTGAAACCATTCTGAGAAGCCAACTTGAAGCATTGGAAAGCATTATAAGTGATAAAGATACTGAAAAACGCTTTTCTCAGGAATTTGAGAAGATGCAGCTCCAGGCAGATGACCTTGCTAATCCAGCATATGCACAAAAAACCCTGAATGAAAGACTTTATCAAAACTCTCAAGCCTTTAAACTTTTGGAAAGCTACTGTGCACAGCACCAGCTTATGATCAATGAAGAAGCGAGAAAAGAGATCGAAAGTCAGATTTTTAATACTGGTAAAGCACCTACAGAAGCAGACTTTAAAGCGATGCAAGAGAAGGGACTACTTATCCCGAATAACGCTAACTTTGAAGAGCTCTCTAAACTCAAGCTCAGCGATGCAGACAAATCCTGA", "aa": "MSQSSTVEKMAIGQNMLQFVLLGVPFSTLAQCCSPATGIVSRIDSSKLKTYYLGEIEKAKANRDSKSETILRSQLEALESIISDKDTEKRFSQEFEKMQLQADDLANPAYAQKTLNERLYQNSQAFKLLESYCAQHQLMINEEARKEIESQIFNTGKAPTEADFKAMQEKGLLIPNNANFEELSKLKLSDADKS", "aa_hexdigest": "69f18685cabe15ba08ded1921c88059b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 21817.482200000006, "isoelectric_point": 5.246659660339354 }, "id": "NKOCPLAKPK_2076", "locus": "GCA003260355_02072" }, { "type": "cds", "sequence": "contig_3", "start": 78086, "stop": 78574, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAGCTAGCGAATAAACCAGAACATCAAGGAATGCAGATCAAGGCACTCAATGAAGAGGAAGTAGAAGTCTCTTCACACGGGAAATCGATCGTCCTCAATATGCAGAAATTGACGCTAGATGGCTTTAAAAACAGTGCCAACATCCCTATCCCTCTCGGAAGTAGGGGGGAACTGCTAAGAGTCGGGCTTCTAGTCAATATGTTGAGAGCAAATGAAGAGATTCGAGGAGAAAAAGTCACTCCTCGCTATGAAGATAAACACGATATGCTCCGACCGTTTGACCTTGCTCATGGACGAGAAGTAGCTTGGAAGGGATTAGGAAGTATCGTCTTCAATGGATTTGAGAAGAGACCGGTCGTGCTAAGTAATTTGATTCCATTTTTTGGTGAGATGGCGAGCGAATTTCCGACGCTAGGAAAAGCGCAGAATAGCGCATTCTTCGTCCAGTATTTGAACCAGCTCCGGAAAAACGATCGTTCTCAATAG", "aa": "MKLANKPEHQGMQIKALNEEEVEVSSHGKSIVLNMQKLTLDGFKNSANIPIPLGSRGELLRVGLLVNMLRANEEIRGEKVTPRYEDKHDMLRPFDLAHGREVAWKGLGSIVFNGFEKRPVVLSNLIPFFGEMASEFPTLGKAQNSAFFVQYLNQLRKNDRSQ", "aa_hexdigest": "0766038a52ded185caab822e6e34d8ab", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18304.875099999983, "isoelectric_point": 9.219833183288571 }, "id": "NKOCPLAKPK_2077", "locus": "GCA003260355_02073" }, { "type": "cds", "sequence": "contig_3", "start": 78613, "stop": 78786, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACTCCAGGATTAACCTCTTCTGAAAGTCCTTTGAATAGGCAAGAAGCTCCTGCTAAAGCTAAAAATTCTCTGAAGAACCTTAAAAATACTGCAGCAATTATTGCAAGCTCTATTGCTATAGTATTAGCTACTACCAGCTGTAAAGGTGCAGCAGAAAAATATGAATCCTGA", "aa": "MTPGLTSSESPLNRQEAPAKAKNSLKNLKNTAAIIASSIAIVLATTSCKGAAEKYES", "aa_hexdigest": "e1b423f25739c60f69e3a6940a17a673", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5877.656999999999, "isoelectric_point": 9.360438728332518 }, "id": "NKOCPLAKPK_2078", "locus": "GCA003260355_02074" }, { "type": "cds", "sequence": "contig_3", "start": 78886, "stop": 79134, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EBP4", "UniRef:UniRef90_A0A328EBP4" ], "nt": "ATGGTTGCCTTGCAAGCAGCTGGAGATATTGGAGGCTACAAAAAAGTCTTTAAAAAAAGTAAACAACTCGAGAAAAGAATTAAAGAAATTGAAAAACAAATTGATAAAAAATCCATGAAGACCCTTGACCTTTCAGATAAGTTTTATAAAGGGAAACAAAAAGCTGAAGAGGAAAAAGCTAGGAATGCTTCTTCCTTCGGAGACTACCAACCAGCAAAACTGCAAAGAAGAACCTTTTTGGAGGACTGA", "aa": "MVALQAAGDIGGYKKVFKKSKQLEKRIKEIEKQIDKKSMKTLDLSDKFYKGKQKAEEEKARNASSFGDYQPAKLQRRTFLED", "aa_hexdigest": "91af18f0cce3807cd3f6f3dfaf529d21", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 78613, "stop": 79140, "observations": { "mutation": [ 78784, 79132 ], "stop": [ 78784, 79132 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EBP4", "query_cov": 1.0, "subject_cov": 0.4685714285714286, "identity": 1.0, "score": 151.0, "evalue": 3.1e-45, "gene_start": 1, "gene_end": 82, "reference_start": 92, "reference_end": 173, "reference_sequence": "MTPGLTSSESPLNRQEAPAKAKNSLKNLKNTAAIIASSIAIVLATTSCKGAAEKYESGEAEQEIAANQAAIDLLIEQYNDYFTDYEKCFDDMVALQAAGDIGGYKKVFKKSKQLEKRIKEIEKQIDKKSMKTLDLSDKFYKGKQKAEEEKARNASSFGDYQPAKLQRRTFLEDGE" }, "paralog": false, "identity": 0.9885714285714285, "score": 334.0, "evalue": 1.37e-119, "description": "Internal stop codon at 78784, 79132. Nonsense mutation around 78784, 79132.", "psc": { "uniref90_id": "UniRef90_A0A328EBP4", "uniref50_id": "UniRef50_A0A328EBP4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EBP4", "UniRef:UniRef50_A0A328EBP4" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EBP4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EBP4" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 9486.843499999997, "isoelectric_point": 9.729326820373537 }, "id": "NKOCPLAKPK_2079", "locus": "GCA003260355_02075" }, { "type": "cds", "sequence": "contig_3", "start": 79217, "stop": 80428, "strand": "-", "frame": 3, "gene": "queA", "product": "tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA", "db_xrefs": [ "EC:2.4.99.17", "GO:0005737", "GO:0006400", "GO:0008616", "GO:0051075", "SO:0001217", "UniRef:UniRef50_A0A328EEW2", "UniRef:UniRef90_A0A328EEW2" ], "nt": "ATGGAGGAAGATCTACTACTCTCTAGTTATACCTATATCCTTCCAGAATCGTGTATTGCACAGCAGCCAAAACTGCCTGAACATAACGCAAAACTCATGATATGTTCTCTCCAAGCCACAGGAGATCTTTTGTATGAAGATCAGATTTTTGCCGATTTGCCAAATTATTTGGATCAACATTATATGCTATTCCTCAATAGTACGAAAGTTTTTAAGGCGAGAATCCCGCTTCACAATACCAAAATCCGCAGAAAAACTGGTCAAGAACTTCTCCTAGAAACAGGAGAACTATTGATCTATGCTATTCATTCAAAGACTCAGTTTGAATGCTTAGTCTCCGATAATAAAAATTTTAGACCAGGAAGCCAAATCTTCCTCGGAGAGGGAGTCGTATTCAGAAGTCTCGAATTTACAGCAAATGGAATCCTTTTTGAAATCTCGAGCGAAAATCCATCCGAAACGAGTGTTCAGATTTTTGACTTTTTGGAAAAAAAGGGAGAAATGCCACTTCCTCCTTACATCCGCTATGAAAAAGAAAAAGAAAAGCGATACCAGACATTTTTTGCGGAGAAACTCGGTTCAGCAGCGGCTCCAACGGCATCTTTACACTTTACTCCCGAACTAGTAAAACGCCTGGAAAAGAAAGGGGTAGAATTCCAATTTCCCTGTCTGCATGTAGGACTAGGAACCTTCAAACCTGTTTATGAAGAGCATATTCAGCAGCAAAAACTGCACTTTGAGCCGATGATCCTAGAGTCAGATATCCGAAAGACGATCGCCGACGCAAAGCTGGCAGGTAAACGTTTCCTCCCAATAGGGACGACAATGATCAGATATTTGGAGTCTTTACCCTATATCCGACGTTTTCTTAAAAAGAAAAATCTGAACATTGCCGTTGATCATGCTACCAGAGATCGACGAGATCACCTCACAAAAACCATAGATGAAAAACTGGTTGAAGAGTTTATTCCAGAGCAGGAGGTGTGCGTTGAGAAAACTCAGTTTTCTTCTATACCAGAAAAGATGATTATCCAAACAAGGCTCTTTATCAGACCAGGAATCCCTTTTCTTTTAACCGATGAACTGATTACGAACTTCCACCTCCCAAAATCTTCATTAATGATCTTGATCTCTGCATTTATAGGAAGAAAAAATCTCTTGGAAGCCTACCAGAGAGCAATCCAAGCAGGATACAAATTCTATAGTTTCTGA", "aa": "MEEDLLLSSYTYILPESCIAQQPKLPEHNAKLMICSLQATGDLLYEDQIFADLPNYLDQHYMLFLNSTKVFKARIPLHNTKIRRKTGQELLLETGELLIYAIHSKTQFECLVSDNKNFRPGSQIFLGEGVVFRSLEFTANGILFEISSENPSETSVQIFDFLEKKGEMPLPPYIRYEKEKEKRYQTFFAEKLGSAAAPTASLHFTPELVKRLEKKGVEFQFPCLHVGLGTFKPVYEEHIQQQKLHFEPMILESDIRKTIADAKLAGKRFLPIGTTMIRYLESLPYIRRFLKKKNLNIAVDHATRDRRDHLTKTIDEKLVEEFIPEQEVCVEKTQFSSIPEKMIIQTRLFIRPGIPFLLTDELITNFHLPKSSLMILISAFIGRKNLLEAYQRAIQAGYKFYSF", "aa_hexdigest": "653b3dc47f6111f0a2a80d68ac95f910", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A328EEW2", "query_cov": 1.0, "subject_cov": 0.9710843373493976, "identity": 0.988, "score": 785.0, "evalue": 2.75e-286, "valid": true, "gene": "queA", "product": "tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA", "ec_ids": [ "2.4.99.17" ], "uniref50_id": "UniRef50_A0A328EEW2", "go_ids": [ "GO:0005737", "GO:0006400", "GO:0008616", "GO:0051075" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EEW2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EEW2" ], "product": "S-adenosylmethionine:tRNA ribosyltransferase-isomerase" }, "genes": [ "queA" ], "id": "NKOCPLAKPK_2080", "locus": "GCA003260355_02076" }, { "type": "cds", "sequence": "contig_3", "start": 80403, "stop": 81038, "strand": "-", "frame": 2, "gene": null, "product": "DUF2238 domain-containing protein", "db_xrefs": [ "KEGG:K08984", "SO:0001217", "UniParc:UPI000DBFF8EB", "UniRef:UniRef100_A0A328EF73", "UniRef:UniRef50_A0A1F9QSR3", "UniRef:UniRef90_A0A328EF73" ], "nt": "ATGTCCAAAAAAATACCACGATTCCCTTGCTGCTTATTAGTAATCTATCTGGTACTCTTCGTTGTTTGTGCTTTTAATCCGCATGATAGATCTGTTTGGTGGGCAGAAAATATTCCTGTGATGGCGGTAGTAGCTTTCTTGGTAATATCCTTTCGCTGGTGGAAGTTTTCTAATCTCTCCTATTTCTTTATGAGTTTACGAATCTTCCTTCATACAATAGGTGGACACTATAGTTTCGAGTTGGTTCCTTTCGGATTTATTAATAATCTCTTTGGCTGGGAGAGGAATATGTATGATAGGATTGCGCATTTTATCATAGGACTTTATGCCTTTCCAATAGTGGAATACCTTCGAAGATCAAAATCAATCCAGAAAAAGTGGCTAGTTTCAACTTTTGGAGTTACAAGTATTCTCGCAATTGCTGGAGCTTACGAAATTTTTGAGTGGTGGTATGCTATTTCTGCTGATCCGGCAGCTGGGATTGCTGTGCTAGGGTCTCAAGGGGATATTTGGGATGCACAAAAAGATATGCTCATGGACACATTGGGAGCAATGTCCGCAGTTTGTTTATTTGCAATTAGAGAGTTTTTAATTAGAAGAAATCTGAATCATGGAGGAAGATCTACTACTCTCTAG", "aa": "MSKKIPRFPCCLLVIYLVLFVVCAFNPHDRSVWWAENIPVMAVVAFLVISFRWWKFSNLSYFFMSLRIFLHTIGGHYSFELVPFGFINNLFGWERNMYDRIAHFIIGLYAFPIVEYLRRSKSIQKKWLVSTFGVTSILAIAGAYEIFEWWYAISADPAAGIAVLGSQGDIWDAQKDMLMDTLGAMSAVCLFAIREFLIRRNLNHGGRSTTL", "aa_hexdigest": "d0035380e1479d0987eaf18f8214b64e", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000DBFF8EB", "uniref100_id": "UniRef100_A0A328EF73", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBFF8EB", "UniRef:UniRef100_A0A328EF73" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EF73", "uniref90_id": "UniRef90_A0A328EF73" }, "psc": { "uniref90_id": "UniRef90_A0A328EF73", "product": "DUF2238 domain-containing protein", "uniref50_id": "UniRef50_A0A1F9QSR3", "kegg_orthology_id": "K08984" }, "pscc": { "uniref50_id": "UniRef50_A0A1F9QSR3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1F9QSR3" ], "product": "DUF2238 domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_2081", "locus": "GCA003260355_02077" }, { "type": "cds", "sequence": "contig_3", "start": 81186, "stop": 81719, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DC03BE5", "UniRef:UniRef100_A0A328EBW7", "UniRef:UniRef50_A0A660MSY0", "UniRef:UniRef90_A0A328EBW7" ], "nt": "ATGACGAAGACAGAGCAACTCCTAAAATCTTTATGCGTGCAGATTAATGCACTTCATATCGCAGGATATCTCAAGGAAGAGGTAATTGCCTCATTAAGATATCTTCATCGATATAAACTTAAAAATTTAGAAAAAAATCTGAATATTTTTTGCGAGTATCTCCTTTTTCTGAATACTCAAAATACCGAGAAGATAGACAATCTTCAGCCTTCTCCCATCCAGAGAAAAAATGCAATGACCTCGCTAGAAATCGACTACTTTATAAAGCCAAAGAAGCAGTTGACACAAACTGCTAAAAAGTACTACTCCAAAAAAATTAGAGATCACGGATATGAATTACTGACCTTAATACAAGGGATAAAAGGTATTTTAGTCCTGAGTATCGATGAATTTTGTAATGCCTCACAAACAAGGATGGGCATTCATAAACTCCTTATGGAAAGAGAGCCTCAATACCAAGAGCTCAAAACAAAAAAGTCAATAAACAAGCCTCTTTCTGACAAACGAAAGATAAAGAAATCCATCAAAAAATAA", "aa": "MTKTEQLLKSLCVQINALHIAGYLKEEVIASLRYLHRYKLKNLEKNLNIFCEYLLFLNTQNTEKIDNLQPSPIQRKNAMTSLEIDYFIKPKKQLTQTAKKYYSKKIRDHGYELLTLIQGIKGILVLSIDEFCNASQTRMGIHKLLMEREPQYQELKTKKSINKPLSDKRKIKKSIKK", "aa_hexdigest": "befd40b9584a7402133e98e5de2ce486", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000DC03BE5", "uniref100_id": "UniRef100_A0A328EBW7", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DC03BE5", "UniRef:UniRef100_A0A328EBW7" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EBW7", "uniref90_id": "UniRef90_A0A328EBW7" }, "psc": { "uniref90_id": "UniRef90_A0A328EBW7", "uniref50_id": "UniRef50_A0A660MSY0" }, "pscc": { "uniref50_id": "UniRef50_A0A660MSY0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MSY0" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 20816.367500000004, "isoelectric_point": 9.772714042663573 }, "id": "NKOCPLAKPK_2082", "locus": "GCA003260355_02078" }, { "type": "cds", "sequence": "contig_3", "start": 81756, "stop": 82106, "strand": "-", "frame": 2, "gene": null, "product": "ABC transporter domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EFP9", "UniRef:UniRef90_A0A328EFP9" ], "nt": "GTGATTTATCCGCTAGTGCTAGAAGGGCTTTCTCTCGCAGAAATCAAAAAAAAGTATGATGCAGTAAATACTTTACTTGATATTTCCTCTATTGAAACCAAGGAAATTAAAAAACTCAGTGGAGGAGAAAAGCAAAAAATCGCGATGGCAAGAGCAATCATTCATGCCCCAGACTTTATTATCGCAGATGAACCGACAGGAAACTTGGATCATGAAAATTCGCTTCAAATCGCTGATATTCTCATAAAAGCAAATCAGATCGGAAATACTATTGTCCTTATTACGCATGATCTTTCTCTATTGGAATATCTTAAAGAACGTACCAAGGTAAAAATTATTACACTTGGTTAA", "aa": "MIYPLVLEGLSLAEIKKKYDAVNTLLDISSIETKEIKKLSGGEKQKIAMARAIIHAPDFIIADEPTGNLDHENSLQIADILIKANQIGNTIVLITHDLSLLEYLKERTKVKIITLG", "aa_hexdigest": "eac645f8f4cf87db00957079eb479abf", "start_type": "GTG", "rbs_motif": "TTTA", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 81756, "stop": 82406, "observations": { "mutation": [ 82160, 82256 ], "stop": [ 82160, 82256 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EFP9", "query_cov": 1.0, "subject_cov": 0.5043478260869565, "identity": 0.983, "score": 216.0, "evalue": 2.05e-69, "gene_start": 1, "gene_end": 116, "reference_start": 115, "reference_end": 230, "reference_sequence": "MTYIQMKAEKNHIPLISIKEMTRGYLDSKRVLFKKFNRELEKGDFTVITGKSGTGKSTLMKFLIGQIKPHKKTVYYKMEDMAEFSDAEIQRYRRKIGIIFQDYQLISSLSPKENVIYPLVLEGLSLAEIKKKYDAVNTLLDISAIETKEIKKLSGGEKQKIAMARAIIHAPDFIIADEPTGNLDHENSLQIADILIKANQIGNTIVLITHDLSLLEYLKERTKVKIITLG" }, "paralog": false, "identity": 0.9861111111111112, "score": 408.0, "evalue": 1.48e-147, "description": "Internal stop codon at 82160, 82256. Nonsense mutation around 82160, 82256.", "psc": { "uniref90_id": "UniRef90_A0A328EFP9", "product": "ABC transporter domain-containing protein", "uniref50_id": "UniRef50_A0A328EFP9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EFP9", "UniRef:UniRef50_A0A328EFP9" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EFP9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EFP9" ], "product": "ABC transporter domain-containing protein" } }, "id": "NKOCPLAKPK_2083", "locus": "GCA003260355_02079" }, { "type": "cds", "sequence": "contig_3", "start": 82254, "stop": 82433, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00005.33" ], "nt": "ATGAAAGCAGAAAAAAACCATATTCCTCTTATTAGTATCAAGGAAATGACTCGAGGATACTTAGACAGTAAAAGAGTGCTTTTCAAAAAATTTAACCGAGAATTAGAGAAGGGGGATTTTACCGTTATTACGGGGAAATCAGGAACGGGAAAATCAACGCTAATGAAATTTTTGATTTGA", "aa": "MKAEKNHIPLISIKEMTRGYLDSKRVLFKKFNRELEKGDFTVITGKSGTGKSTLMKFLI", "aa_hexdigest": "b254d82b9b0afe5df67347d1c1263fe0", "start_type": "ATG", "rbs_motif": "TTCTT", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00005.33", "name": "ABC_tran", "length": 30, "aa_cov": 0.5084745762711864, "hmm_cov": 1.0, "evalue": 7.575374859943111e-07, "score": 26.27985191345215, "start": 29, "stop": 58 } ], "seq_stats": { "molecular_weight": 6776.000999999998, "isoelectric_point": 10.073716926574708 }, "id": "NKOCPLAKPK_2084", "locus": "GCA003260355_02080" }, { "type": "cds", "sequence": "contig_3", "start": 82776, "stop": 83420, "strand": "-", "frame": 2, "gene": null, "product": "Periplasmic heavy metal sensor", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MTI5", "UniRef:UniRef90_A0A660MTI5" ], "nt": "ATGAGTCTCGCACGGATCAACTATGCCACGCTCACTGAAAATCAAAAGTTACAGTTGGCAGAAATTATCAGAGTATACAAAGACAAGCTCAAAATTCTCTTCACTTCAGAGAGCCAAGAAAATACTACTGAAGAGCAGACAAAACTCAAACAGCAAAAGGATCAAATTATTATCGAACTTTTCAAGAATCTTGCCCCTTTTGTTGCTCCAAATAAACAAGCAGAGTTTCAGCAGTTCCAATCACAGAATTTCCTCTCGCTCCTTGAAGAGGAAGGGAAAAAGCAAAAAAAAGGTAAAAAGAATACGCTAAAAGGTGAACAATTAGGCTTCTTATGGGGAATGTTAGATGAAAATAGCCTAACAGACAAACAAAAAGAAGAGATTGCTCTCCTTCAGGTCCAAAAACAGCAAAAAATGGAGCAAATCCTCCAAAGATTGCCAAGAGCTTCCGAAAAAGAAAAGACTGAATTAGAAGAGCAGTTGAAGCAAATTAATAGCGATTTCCTTATGGCACTAGAAGCGTATATTCCCGCAGATAAAGTATCAGAGTTTGAAGCTTTTATAGAGGAAATCCCAACTTGGAATCAGAAAGCTGAAAAAAAGCCTGCTTCACCAAAAAAAGAAACCGCTGCCTTAGCAGCCTAA", "aa": "MSLARINYATLTENQKLQLAEIIRVYKDKLKILFTSESQENTTEEQTKLKQQKDQIIIELFKNLAPFVAPNKQAEFQQFQSQNFLSLLEEEGKKQKKGKKNTLKGEQLGFLWGMLDENSLTDKQKEEIALLQVQKQQKMEQILQRLPRASEKEKTELEEQLKQINSDFLMALEAYIPADKVSEFEAFIEEIPTWNQKAEKKPASPKKETAALAA", "aa_hexdigest": "00bffad2fcc1e34f6db320cdfd8e8de0", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 82776, "stop": 83720, "observations": { "mutation": [ 83441 ], "stop": [ 83441 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A660MTI5", "query_cov": 1.0, "subject_cov": 0.6062322946175638, "identity": 0.972, "score": 374.0, "evalue": 2.14e-128, "gene_start": 1, "gene_end": 214, "reference_start": 140, "reference_end": 353, "reference_sequence": "MKSSKFLASVVVFSVITMTGFSSRAATTESFEAPSFPYLRPNLEASQSLKSESTIWSQLSLSEEKKNQLDALLQQLKTERSTYQEQREKATTDREKILLKMQWEARKMEIRDAILDLIPAAQKLQIQKILSKGKGGVVAMSLARINYATLTENQKLQLAEIIRVYKDKLKILFTSDNQENTTEEQIKLKQQKDQIIIELFKNLAPFVAPNKQAEFQQFQSQNFLSLLEEEGEKQKKGKKNTLKGEQLGFLWGMLDENSLTDKQKEEIALLQVQKQQKMEQILQRLPTASEKEKTELEEQLKQINSDFLMALKAYIPADKVSEFEAFIEEIPTWNQKAEKKPASPKKETAALAA" }, "paralog": false, "identity": 0.9554140127388535, "score": 572.0, "evalue": 8.2e-209, "description": "Internal stop codon at 83441. Nonsense mutation around 83441.", "psc": { "uniref90_id": "UniRef90_A0A660MTI5", "product": "Periplasmic heavy metal sensor", "uniref50_id": "UniRef50_A0A660MTI5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A660MTI5", "UniRef:UniRef50_A0A660MTI5" ] }, "pscc": { "uniref50_id": "UniRef50_A0A660MTI5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MTI5" ], "product": "Periplasmic heavy metal sensor" } }, "id": "NKOCPLAKPK_2085", "locus": "GCA003260355_02081" }, { "type": "cds", "sequence": "contig_3", "start": 83439, "stop": 83837, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAGTAGCAAATTTCTTGCCTCAGTGGTTGTGTTTTCAGTGATCACGATGACGGGTTTCTCTTCCCGGGCAGCGACAATAGAGAGTTTTGAGGCGCCTAGTTTTCCTTATCTTCGTCCAAATCTAGAAGCTTCCCAAAGTCAAAAATCTGAATCAACAATCTGGAGTCAGCTTTCACTTAGTGAAGAAAAAAAGAATCAGCTTGACGCACTTCTCCATCTGCTCACAACAGAGAGGAGTATTTACCAGGAACAGCGAGAAAATGCAACTACCGATAGAGAAAAAATCCTCCTCAAAATGCAGTGGGAGGCGAGAAAAATGGAGATCAGAGACGCTATCCTAGACTTAATCCCAGCGGCTCAGAAGTTGCAGATTCAGAAAATATTGTCCAAGTGA", "aa": "MKSSKFLASVVVFSVITMTGFSSRAATIESFEAPSFPYLRPNLEASQSQKSESTIWSQLSLSEEKKNQLDALLHLLTTERSIYQEQRENATTDREKILLKMQWEARKMEIRDAILDLIPAAQKLQIQKILSK", "aa_hexdigest": "7b1021bd7041899e5163e37d88868bf6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15106.222299999983, "isoelectric_point": 8.924310111999514 }, "id": "NKOCPLAKPK_2086", "locus": "GCA003260355_02082" }, { "type": "cds", "sequence": "contig_3", "start": 84053, "stop": 84241, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAGAGCTCCTCAAAGGACTTCGTTAGTATTTGGAGATCTTACACTGGATCTTTCTCAGAAAAAAGTTTTTAAAGCAGGATCAGAACTGAAGTTTTCTCTCAAGGAATTGCAGATTTTAGAGCTTCTAATGAGAAATCACGGGATCGCAGTTTCTAGGCTAGAAATCATTGAAGAAATTCGATGA", "aa": "MKRAPQRTSLVFGDLTLDLSQKKVFKAGSELKFSLKELQILELLMRNHGIAVSRLEIIEEIR", "aa_hexdigest": "650164b1bb1a84489310ce5daba13816", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7139.390999999995, "isoelectric_point": 9.691741752624512 }, "id": "NKOCPLAKPK_2087", "locus": "GCA003260355_02083" }, { "type": "cds", "sequence": "contig_3", "start": 84305, "stop": 84505, "strand": "-", "frame": 3, "gene": null, "product": "Response regulatory domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A351Y3R6" ], "nt": "TTGCGGGTCCAAAATGGAAGCTATGCAAATGAAGTAGTAAAACATCAGACTTTTGATCTAATTTTACTGGATCTTATGCTTCCCGGGACAGACGGAATCGCTATTGCAAAAACAATTAGAAAAATCCAGCCTCAGACGCCAATTTTGATGATGACAGCAAAAGGGCAATTGGAAGATAAATTGGAGGGATTCGAGGTGTGA", "aa": "MRVQNGSYANEVVKHQTFDLILLDLMLPGTDGIAIAKTIRKIQPQTPILMMTAKGQLEDKLEGFEV", "aa_hexdigest": "d5f81c7e7e7f5b0e90ee6561e9136820", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A351Y3R6", "query_cov": 0.8333333333333334, "subject_cov": 0.9821428571428571, "identity": 0.655, "score": 78.2, "evalue": 6.35e-18, "valid": false, "product": "Response regulatory domain-containing protein", "uniref50_id": "UniRef50_A0A351Y3R6" }, "pscc": { "uniref50_id": "UniRef50_A0A351Y3R6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A351Y3R6" ], "product": "Response regulatory domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_2088", "locus": "GCA003260355_02084" }, { "type": "cds", "sequence": "contig_3", "start": 84784, "stop": 85470, "strand": "-", "frame": 1, "gene": null, "product": "histidine kinase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EB22", "UniRef:UniRef90_A0A328EB22" ], "nt": "ATGGCACTACTTCGGGTAACTTTGGTTCTTGTTCTAGTAGGATCAGCTTTTGCATATGGTTTTTCTTTTCTTTTTGTGAAAAAAGCACTTAAAAAGCTTAAAAAACTGAATCAAGCTCTCGAAAAACTAGATATTGATCATCTAAATCAACCACTCTTAGTCGAAGGCAATCCCAAGGATGAAATCAATAAAGTGAGTCAAAAGTTTAATCTTGCACTAGCTAAAATCCAAAGGCAAACTCTAGGGCTCAAGGACTTTATCAAAAATGCCTCTCATGAGCTCAAAACCCCGCTAATGACGATTTTTACGCTTTCAGACTTGGCAAAGAAGACTAAGGATTATGAAGCGATCATCAAAAAGATTAAGGCAGAAGCGAAATCTATGGATCAGCTTTTAGAAACACTCCTTCTTATCACGCAAATCGAGGACAAAACAGAACTTCCCAAGCAGGAGCTTAACCTTTCTCAGAGCTTAAAAGGACTCTTAGAGCAGTATCAGCTTACCTATCAGGAAAAAAATCTGAACTTCATCTCTGATTTTCCTCCATCACTCACAAAAAAAGTGCATCCCCAAGGACGAACCACAATTGTGAGTAATCTTCTCTCTAATGCCTGTAAATTTACACCTCAAGGGGGAACGATTCAAATTTCCCTCAATGACCAAGAATTGAAAATTTCTGATACGTGA", "aa": "MALLRVTLVLVLVGSAFAYGFSFLFVKKALKKLKKLNQALEKLDIDHLNQPLLVEGNPKDEINKVSQKFNLALAKIQRQTLGLKDFIKNASHELKTPLMTIFTLSDLAKKTKDYEAIIKKIKAEAKSMDQLLETLLLITQIEDKTELPKQELNLSQSLKGLLEQYQLTYQEKNLNFISDFPPSLTKKVHPQGRTTIVSNLLSNACKFTPQGGTIQISLNDQELKISDT", "aa_hexdigest": "3283f8c7bbfd7b01350c6776be953a4f", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 84595, "stop": 85770, "observations": { "mutation": [ 84786, 85518, 85563 ], "stop": [ 84786, 85518, 85563 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EB22", "query_cov": 1.0, "subject_cov": 0.5520581113801453, "identity": 0.987, "score": 421.0, "evalue": 1.67e-145, "gene_start": 1, "gene_end": 228, "reference_start": 123, "reference_end": 350, "reference_sequence": "MRNSLTTSKKISLQFTIFSAILLCVFGILVNIIFFLSWYALRDPERPRHDDDEESSFAVHIPLHKEFPRHELRFDPDDPEVEELLDAKLWNGVAKLDDYYLHYVIAGDKVIIRNISSQIEAQMALLRVTLVLVLVGSAFAYGFSFLFVKKALKKLKKLNQALEKLDIDHLNQPLLVEGNPKDEINKVSQKFNLALAKIQRQTLGLKDFIKNASHELKTPLMTIFTLSDLARKTKDYEAIIKKIKAEAKSMDQLLETLLLITQIEDKTELPKQELNLSQSLKGLLEQYQLTYQEKNLNFISDFPPSLTKKVHPQGRITIVSNLLSNACKFTPQGGTIQISLNDQELKIADTGPGISKEQQEKIWERFWQAEESHTDTKSFGLGLYLAQLFTQKQGFQLAVSSVLGQGTTFILKF" }, "paralog": false, "identity": 0.9820971867007673, "score": 754.0, "evalue": 1.24e-279, "description": "Internal stop codon at 84786, 85518, 85563. Nonsense mutation around 84786, 85518, 85563.", "psc": { "uniref90_id": "UniRef90_A0A328EB22", "product": "histidine kinase", "uniref50_id": "UniRef50_A0A328EB22", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EB22", "UniRef:UniRef50_A0A328EB22" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EB22", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EB22" ], "product": "histidine kinase" } }, "id": "NKOCPLAKPK_2089", "locus": "GCA003260355_02085" }, { "type": "cds", "sequence": "contig_3", "start": 85561, "stop": 85836, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCGAAATTCCCTCACAACCTCCAAAAAAATTTCACTGCAGTTTACAATCTTTTCGGCAATTCTGCTCTGTATTTTTGGGATTTTGGTAAATATCATTTTTTTTCTCAGTTGGTATGCATTACGAGATCCGGAACGTCCTCGTCATGACGACGATGAGGAGTCCTCTTTTGCCGTGCATATCCCTCTTCATAAAGAGTTTCCTCGCCATGAGCTCCGTTTCGATCCGGATGATCCAGAGGTCGAAGAGTTGCTGGATGCAAAACTCTGGAATTGA", "aa": "MRNSLTTSKKISLQFTIFSAILLCIFGILVNIIFFLSWYALRDPERPRHDDDEESSFAVHIPLHKEFPRHELRFDPDDPEVEELLDAKLWN", "aa_hexdigest": "d663466858db7ae9b543d686d4495bd1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10785.19829999999, "isoelectric_point": 5.037605857849121 }, "id": "NKOCPLAKPK_2090", "locus": "GCA003260355_02086" }, { "type": "cds", "sequence": "contig_3", "start": 85937, "stop": 86164, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTTCTCCGGTATGTCAGTCAGGTTTGATTTTTTTCAATACCCTTTTTGATGAGAACGCAAGTTGCCATTTCGCTCTGGGAAACTCCTATCCTACAACAATCAAGGACGGCATAACTCTTGATGATGCAGGCAAAGAAAAGGCAGGGATGAACCAAAGTATTACACATGTAGATTTTATGGTGGGTGACAAAAGCTTGACGATCACTGGAGAAACTGTTTCATGA", "aa": "MSSPVCQSGLIFFNTLFDENASCHFALGNSYPTTIKDGITLDDAGKEKAGMNQSITHVDFMVGDKSLTITGETVS", "aa_hexdigest": "1416f6c9847d99cae6d77965936f0c45", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8005.885, "isoelectric_point": 4.503944587707521 }, "id": "NKOCPLAKPK_2091", "locus": "GCA003260355_02087" }, { "type": "cds", "sequence": "contig_3", "start": 86198, "stop": 86392, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCTACAGAGGAGGTCTTTACTGCACCTCATAAATATGGAGTAAATGGAACTTTAGGAGCGACTTTGCCACTCTATTACAACGGAAAACTGATTGAAGATTTCGGTTTTACATTTAAGGATGGAGCAGTTGTCGACTTCTACGCCAAAAAGGGATACGAAACGTTGAAATCCCTTTTAGAAACCGATCCTTGA", "aa": "MPTEEVFTAPHKYGVNGTLGATLPLYYNGKLIEDFGFTFKDGAVVDFYAKKGYETLKSLLETDP", "aa_hexdigest": "cd736611b192f282195767ab5e3459f2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7109.965100000002, "isoelectric_point": 4.834633827209472 }, "id": "NKOCPLAKPK_2092", "locus": "GCA003260355_02088" }, { "type": "cds", "sequence": "contig_3", "start": 86675, "stop": 87127, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAAATCTTCAAGCAAAAAAGAAAAAATATGCTGAATTGATAATCCAGTTTGGACTCAATTTACAGCAGAGACAAAATCTCTTGATCAAAGCTCCGATTGATGCTTTTGACTTTGTTAAACTGGTAGTAGAAGAGGCATATAACGCAGGAGTAGATCAGATTTTTTATCAATGGAGGAGTGATGAACTCGATCTTTTGAAATATCAAAAGGCTTCAGTCGCAAGCTTTGGAAAACCGCTTCAGCGATTAGAAGATGGCTACGCTACAGAACTTAAAAAAAATTGTGCGGTGCTGACCTTATTTTCCGAAGACCCTTTTCTCTTTAGTCAAGTGCAGCCAGAGTTGCTCAATATGGAGCTTCAGGCGAGAAGAGCTGCTCGTAAAGCGCAGCTAGAACTCATTTCCTCAAATGCAATCAATCGACTTATCGCTGCGGTTCCAAACCTATGA", "aa": "MQNLQAKKKKYAELIIQFGLNLQQRQNLLIKAPIDAFDFVKLVVEEAYNAGVDQIFYQWRSDELDLLKYQKASVASFGKPLQRLEDGYATELKKNCAVLTLFSEDPFLFSQVQPELLNMELQARRAARKAQLELISSNAINRLIAAVPNL", "aa_hexdigest": "7aaf91ef1bc77e7fcd2388a210869b7b", "start_type": "ATG", "rbs_motif": "TTCTT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17146.656000000003, "isoelectric_point": 8.642454338073733 }, "id": "NKOCPLAKPK_2093", "locus": "GCA003260355_02089" }, { "type": "cds", "sequence": "contig_3", "start": 87155, "stop": 87367, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAAGGCGTTTACGCATTGGCATTATATTACTTTGCGAGGATACAACGATAGAGAGCAAATTTTTTACGTCTATGATTCCAATACCAAAGGAGTGACAGAGCAATACCTTATGAAGGGAACGGTGAAAATTCCCTATGCAGTACTTCTCAAGTCTCGATCCTTTGGTGCTTCTAAATTATTGAGAAATTATGCAATCGGTGTTGAGTATTAG", "aa": "MKAFTHWHYITLRGYNDREQIFYVYDSNTKGVTEQYLMKGTVKIPYAVLLKSRSFGASKLLRNYAIGVEY", "aa_hexdigest": "73aa2811cc7b24177d3bd3ae03b6cb1f", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8245.4054, "isoelectric_point": 9.600132179260253 }, "id": "NKOCPLAKPK_2094", "locus": "GCA003260355_02090" }, { "type": "cds", "sequence": "contig_3", "start": 87650, "stop": 87790, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTGCCCAAGACCGCTTATTTAAGATTCTAGCTGATGCTCCTTCTCATACCAATCTGACCAAACCTATAGAGGACGATTTTTTCCTGATTACAAAGACGCCACCTGAGGGATTCATGCATAGAAAGGCAAAGTTTTGA", "aa": "MLAQDRLFKILADAPSHTNLTKPIEDDFFLITKTPPEGFMHRKAKF", "aa_hexdigest": "6a9b61aa49a4599a4291101d73ef18e3", "start_type": "ATG", "rbs_motif": "TTCTT", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5331.1746, "isoelectric_point": 8.18105411529541 }, "id": "NKOCPLAKPK_2095", "locus": "GCA003260355_02091" }, { "type": "cds", "sequence": "contig_3", "start": 88069, "stop": 88509, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00395.25" ], "nt": "TTGGCGAGCCTCAATATTGTAAAAGGCTATCCTGATGGGAGTTTTAAGCCAAAGCAGGCGATTACCCGTGCAGAGCTTCTAAAAATCATCTTACTTTCTGCGAATATTGATACAGCAGGAGAACATGAAACTTGCTTTCTTGATGTCGCTAAGGAAGATTGGTTTGTGGACATCGTATGCACTGCGAAAGCTATGGGAATCGTAAAGGGCTATGATGACGGCACGTTTAAGCCGAATCAGACAGTTACTTTTGTGGAAGGGCTGAAAATGGCGATCGAAGGTTTTAAAATCCAAACGAGAAACGTCGATTCAAACTTTTGGTATGCAAAGTATCTTGATTTTGTGCATCAGCATTCAATTTTTTCTCAATATGCTTACTATCCAGAACAGGCATTTAGTCGTGAGATGATGGCTCATTTAGCAACAATGCTCCTCAAATGA", "aa": "MASLNIVKGYPDGSFKPKQAITRAELLKIILLSANIDTAGEHETCFLDVAKEDWFVDIVCTAKAMGIVKGYDDGTFKPNQTVTFVEGLKMAIEGFKIQTRNVDSNFWYAKYLDFVHQHSIFSQYAYYPEQAFSREMMAHLATMLLK", "aa_hexdigest": "97c0c5f84365694f7d9b4e09cf7a3520", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00395.25", "name": "SLH", "length": 39, "aa_cov": 0.2602739726027397, "hmm_cov": 0.9743589743589743, "evalue": 5.591415565072918e-26, "score": 86.7666244506836, "start": 46, "stop": 83 } ], "seq_stats": { "molecular_weight": 16588.94259999999, "isoelectric_point": 6.057013130187988 }, "id": "NKOCPLAKPK_2096", "locus": "GCA003260355_02092" }, { "type": "cds", "sequence": "contig_3", "start": 88594, "stop": 88812, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTAATGGAACAGAAAGGCACTTTATCACCCATGTAGGACAGCATTATACTCCTTCTCAACCTGCAAAATTAGTGTTTGCCTTTCATGGACGCACCAGCTCTAATGATGGACTGGGGTATTATGGAATTGAGGAAGCTAGTGATGGGAATTTGATTACCGTTTATCCAGCAGGATTACCAGAGGATGGTCCTCAGAGGAATCGAAGAGATCCGTGA", "aa": "MLNGTERHFITHVGQHYTPSQPAKLVFAFHGRTSSNDGLGYYGIEEASDGNLITVYPAGLPEDGPQRNRRDP", "aa_hexdigest": "c6569ded482d835068290587e6156c6e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7955.634099999997, "isoelectric_point": 6.00705165863037 }, "id": "NKOCPLAKPK_2097", "locus": "GCA003260355_02093" }, { "type": "cds", "sequence": "contig_3", "start": 88828, "stop": 89082, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAGAGATTATCAGCTTTTTGATGCTATTTTGGATGAAATCTCTGAGCACTATTGTATTGATCCGAGTGAAGTATACGTGGTAGGACACTCATTGGGTGGGCGATTTACCAGCATGCTCGGATGTGCGAGAGCAACGAATATCCATGGTGTTGGTATCGTAGCGGGAAGCCCCATGCCGTTTCCTGAGTGTTCTGCACCGACCTCTGCGATCATCTTTCATAATCCGAATGATAACCTTGCATCTTTTGCATGA", "aa": "MRDYQLFDAILDEISEHYCIDPSEVYVVGHSLGGRFTSMLGCARATNIHGVGIVAGSPMPFPECSAPTSAIIFHNPNDNLASFA", "aa_hexdigest": "4162d8b562f3669473aefc108f371e6c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9044.11729999999, "isoelectric_point": 4.749602699279785 }, "id": "NKOCPLAKPK_2098", "locus": "GCA003260355_02094" }, { "type": "cds", "sequence": "contig_3", "start": 89108, "stop": 89308, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCATGGGGAGGAAGATGCTACTTTTCTTTCCAAAATTTTCGCATCATGTTGGCAGCTCCGTTTGGTCGCATATGCCCACCCTCAGAATACTTACAAAATACTACAGGAGCTTGAGGGAGACAGTTTGAATATCTTACGCAGTCCATCCCATAGGAGTTCTGGTAAACTTCGGTTTCTGGTCAGCATTGGTTTTGTTTGA", "aa": "MHGEEDATFLSKIFASCWQLRLVAYAHPQNTYKILQELEGDSLNILRSPSHRSSGKLRFLVSIGFV", "aa_hexdigest": "9c5466c6a58b77511b8dabc10034cc78", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7507.522099999997, "isoelectric_point": 7.964118003845215 }, "id": "NKOCPLAKPK_2099", "locus": "GCA003260355_02095" }, { "type": "cds", "sequence": "contig_3", "start": 90021, "stop": 90551, "strand": "+", "frame": 3, "gene": null, "product": "Polymerase nucleotidyl transferase domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EF83", "UniRef:UniRef90_A0A328EF83" ], "nt": "TTGACAGGTTCTCGTGCGATAGGTACCTGGACTCCAACTTCGGATATAGATATTCTTGTTTTTATTGATCCGAAAGACTACTCGTATTATTTAAAGCAATCTTTTGAACATGGTTTTAGAAAAGAAGGTGAAGAGGCGGGGCTAGAAGAACGATTTTCTTATGAAGGAAGCGAGGTTCTTCTCGAAATAAAGTTTATGACAGAACTCTATCTTAGCAACCTCATTGACTATTTTTCTTATAGCAAGATGCAATTGGTTAATGACAATCACGCCAAACACAGCTCGCTTAAAGCAGACTTGCAAAAATGGTTTGATCAAGGGTATGAAGACAGACTCATGCAGCATTATATCCAAGCATTTCAAGACATGAAAGAGCTTAAAGATATGTTCAAAAAAGAACAAAAAAACTTGATGCTGGATACTATCTTTATCAAGAAAGGAATATTTCTGGAAGCTGTAATGAAGCTTCTCTATCTTATAAAAAGACAAGGCTATCCAACAGTTAAATGGATGTTACCTCTCTTAGACTGA", "aa": "MTGSRAIGTWTPTSDIDILVFIDPKDYSYYLKQSFEHGFRKEGEEAGLEERFSYEGSEVLLEIKFMTELYLSNLIDYFSYSKMQLVNDNHAKHSSLKADLQKWFDQGYEDRLMQHYIQAFQDMKELKDMFKKEQKNLMLDTIFIKKGIFLEAVMKLLYLIKRQGYPTVKWMLPLLD", "aa_hexdigest": "51228a796aa4defbe3f82bb053efc298", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 89943, "stop": 90725, "observations": { "mutation": [ 89997, 90549 ], "stop": [ 89997, 90549 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EF83", "query_cov": 1.0, "subject_cov": 0.676923076923077, "identity": 0.9940000000000001, "score": 348.0, "evalue": 5.9e-120, "gene_start": 1, "gene_end": 176, "reference_start": 27, "reference_end": 202, "reference_sequence": "MPETLKQLILQFMGTIYPGIEKEAIFLTGSRAIGTWTPTSDIDILVFIDPKDYSYYLKQSFEHGFRKEGEEAGLEERFSYEGSEVLLEIKFMTELYLSNLIDYFSYSKMQLVNDNHAKHSSLKADLQKWFDQGYEDRLMQHYIQAFQDMKELKDMFKKEQKNLMLDTIFIKKGIFLEAVMKLLYLIKRQGYPTVKWMLPLLDGEEGTWIQEIVSEVKSLATLEEFLIMEEKIKRQINKKLMPQRPYVGQRWKFLSAFKHL" }, "paralog": false, "identity": 0.9923076923076923, "score": 517.0, "evalue": 3.64e-189, "description": "Internal stop codon at 89997, 90549. Nonsense mutation around 89997, 90549.", "psc": { "uniref90_id": "UniRef90_A0A328EF83", "product": "Polymerase nucleotidyl transferase domain-containing protein", "uniref50_id": "UniRef50_A0A328EF83", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EF83", "UniRef:UniRef50_A0A328EF83" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EF83", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EF83" ], "product": "Polymerase nucleotidyl transferase domain-containing protein" } }, "id": "NKOCPLAKPK_2100", "locus": "GCA003260355_02096" }, { "type": "cds", "sequence": "contig_3", "start": 90827, "stop": 90922, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATACGAGAACAGGATATGAAGATATGTTACGTAAGATCCATGAAAAAACTGCTTTTGTAGCTGAAGAATACAAAAAGATGCTTAATGAGTGA", "aa": "MNTRTGYEDMLRKIHEKTAFVAEEYKKMLNE", "aa_hexdigest": "7ccc850582b537fe104cfd817161b5ee", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3777.3086000000003, "isoelectric_point": 6.556400489807129 }, "id": "NKOCPLAKPK_2101", "locus": "GCA003260355_02097" }, { "type": "cds", "sequence": "contig_3", "start": 91049, "stop": 91204, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCTTGCAGCGAATCTTTCCTCTTGTTCTGTCTATGATTCTAAAATAGTTACCCTAGATCCAAAGGGAGAATCGGTTACTTTTTCGGTAATTTACAAGGGATACAAAGACGGTCATCAGGACTTTGCTGTAGAAGTCAAGATCAAAAAAACCTGA", "aa": "MLAANLSSCSVYDSKIVTLDPKGESVTFSVIYKGYKDGHQDFAVEVKIKKT", "aa_hexdigest": "369b3dc8b045a9d558948ad7bccdd18a", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5612.3861000000015, "isoelectric_point": 7.853683662414551 }, "id": "NKOCPLAKPK_2102", "locus": "GCA003260355_02098" }, { "type": "cds", "sequence": "contig_3", "start": 91712, "stop": 91936, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTCTAGTATCGCAATACCTCACGCACCGAGTGTTCTTGGAGCTCCAGCAAGACCATTTCCAACACTCCTTCCAATACTCGTTATGGTTTTTCCCATCTTGGATATAAAAAAAGTAAAATTAGTCTTCTATGTCAAGATTTCTCAGATATTCGTCACGAAAATCTTCAAAAATCTGAGCCAAAAAATCATCATAGTTTGGAATCAGATTTTCTATTTTTTCTAA", "aa": "MSSIAIPHAPSVLGAPARPFPTLLPILVMVFPILDIKKVKLVFYVKISQIFVTKIFKNLSQKIIIVWNQIFYFF", "aa_hexdigest": "5cb9efef742cb6aa87b38c5beed81d6d", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8490.371299999999, "isoelectric_point": 10.296906471252438 }, "id": "NKOCPLAKPK_2103", "locus": "GCA003260355_02099" }, { "type": "cds", "sequence": "contig_3", "start": 91832, "stop": 92233, "strand": "-", "frame": 3, "gene": null, "product": "DUF2059 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4KT32", "UniRef:UniRef90_A0A660MSF8" ], "nt": "ATGCAGAGTTTTCTGAGGAAGAAAAGGAATAAAAATCAGAATTTAAAAGCTATTTTACCCATTACAAAGAAAAAGATGCCACATCAACACCTAGAAGAAACCCATGAAGCGGACTTTCTCAATGATCTGCTTTTAGAAGCTGGTTTTGATCCGCAAAAAGATGATTTTGAAGAGCTTAAGTCTGACATAGAGCCTATCCTTATGGATAGAATTATGATGAAGGTTTTTGAGACACTTTCCCCAGCTCAGAGGAAGGATATTATGAAGCTTTTTGACGCAGGAAAAGAAGCAGAAGCATTAGAAAAAATAGAAAATCTGATTCCAAACTATGATGATTTTTTGGCTCAGATTTTTGAAGATTTTCGTGACGAATATCTGAGAAATCTTGACATAGAAGACTAA", "aa": "MQSFLRKKRNKNQNLKAILPITKKKMPHQHLEETHEADFLNDLLLEAGFDPQKDDFEELKSDIEPILMDRIMMKVFETLSPAQRKDIMKLFDAGKEAEALEKIENLIPNYDDFLAQIFEDFRDEYLRNLDIED", "aa_hexdigest": "90ff172485a954bc5d3e08dd584c203f", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A660MSF8", "query_cov": 0.8120300751879699, "subject_cov": 1.0, "identity": 0.981, "score": 208.0, "evalue": 1.22e-67, "valid": true, "uniref50_id": "UniRef50_A0A2W4KT32" }, "pscc": { "uniref50_id": "UniRef50_A0A2W4KT32", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4KT32" ], "product": "DUF2059 domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_2104", "locus": "GCA003260355_02100" }, { "type": "cds", "sequence": "contig_3", "start": 92202, "stop": 92507, "strand": "-", "frame": 2, "gene": null, "product": "Trigger factor C-terminal domain-containing protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBFD253", "UniRef:UniRef100_A0A328EF52", "UniRef:UniRef50_A0A328EF52", "UniRef:UniRef90_A0A328EF52" ], "nt": "ATGTTGAAAAATCTAGCAGAAGCAAAAGAGTTTGCAGTAGAGAAAATCGAAGAAATCGTAGAAGATAAGCTTTCTGATTGGGAAAAAGACCTTATAGAATTTAAAATTGAAGATGATTTTTATCATAAGCTTGAGGAGATTGTCTCAGATGAAGAGATAGAAAATGCTGGATTAGCATCGCAGGAAGAACTAGATGCTTACCTTTTTACACACGTCCCGAATTATAATGCTATTCTAGAAGACGTCACTGCTAATTTCCTCGCTGAATATATGAATGCAGAGTTTTCTGAGGAAGAAAAGGAATAA", "aa": "MLKNLAEAKEFAVEKIEEIVEDKLSDWEKDLIEFKIEDDFYHKLEEIVSDEEIENAGLASQEELDAYLFTHVPNYNAILEDVTANFLAEYMNAEFSEEEKE", "aa_hexdigest": "41a8f3be624fbecb782952d2d2517cc1", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000DBFD253", "uniref100_id": "UniRef100_A0A328EF52", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBFD253", "UniRef:UniRef100_A0A328EF52" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EF52", "uniref90_id": "UniRef90_A0A328EF52" }, "psc": { "uniref90_id": "UniRef90_A0A328EF52", "product": "Trigger factor C-terminal domain-containing protein", "uniref50_id": "UniRef50_A0A328EF52" }, "pscc": { "uniref50_id": "UniRef50_A0A328EF52", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EF52" ], "product": "Trigger factor C-terminal domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_2105", "locus": "GCA003260355_02101" }, { "type": "cds", "sequence": "contig_3", "start": 92541, "stop": 93353, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DC04EF5", "UniRef:UniRef100_A0A328EB30", "UniRef:UniRef50_A0A328EB30", "UniRef:UniRef90_A0A328EB30" ], "nt": "ATGGCATACAATAATTACAGTAGCTATTCTGCAAGTACCCTAGATGCTAAAGATAAGCATTCAGACGCAGAGCTAAGAAAGATGGATGAAGCTGAGCTCAAAAGACAGCTAAAACTCGCAATTGATGGGAACGATGACGCCTATTTCAATCTTGTAAACCAAGTTTTAGACGAGAAGATCAAAAATAAGGCAAATAGTATCAATGATTTTCAATCGGCAGAAGGGGCGACCCAGACAATGAAATCTAGGTATGAAGCAATCCAAAATATGCCCGATACAACGCCTGCAGAAAAGGATGCTAAAAATAGTGCCATAAAGGATTTCTTTGAAAATGAGATGAAAAACTGGACTTCAAAGGCAGATAATATCGTCGATTTTGCTAAATCTCAAAAAGGCGAGGCAGACAGGTTGAAGAATCTGCTTGAAGGCGCAATAACTAAAAATGTAAAACTCAAAGAATCTCTGCCTTTTGGGAAACGGGGAAGATTAAAAAGAATCTACCATCAAGTGCAGAAAATCAAAGAAAAATACCCAGAAGATCGAGGAGAGCGTGTTCTTTTTCACCTAATGTCACTTACAGGCAAAAACTACACAGGCATGCTTCAAGGATGGAGAAGGAATATAAGAGCCGGGGCAAGGCGAAGAAAGTATTCAAAAGATATTGATGGGAATCTTGCAGTTATCGAAGAAAAAATAAAGCCTGATCCAAAAGATGGTGTTGTAGGAACCTTAGTAAAAGAACGTCTCTCTCATCTCATAGAAGAGGCTAAAGATCATTATTTTGAGAGCCAACGCAAAAGTATCTATGGATAA", "aa": "MAYNNYSSYSASTLDAKDKHSDAELRKMDEAELKRQLKLAIDGNDDAYFNLVNQVLDEKIKNKANSINDFQSAEGATQTMKSRYEAIQNMPDTTPAEKDAKNSAIKDFFENEMKNWTSKADNIVDFAKSQKGEADRLKNLLEGAITKNVKLKESLPFGKRGRLKRIYHQVQKIKEKYPEDRGERVLFHLMSLTGKNYTGMLQGWRRNIRAGARRRKYSKDIDGNLAVIEEKIKPDPKDGVVGTLVKERLSHLIEEAKDHYFESQRKSIYG", "aa_hexdigest": "52779bffeb1fe01a5beb23f9016c87c1", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000DC04EF5", "uniref100_id": "UniRef100_A0A328EB30", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DC04EF5", "UniRef:UniRef100_A0A328EB30" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EB30", "uniref90_id": "UniRef90_A0A328EB30" }, "psc": { "uniref90_id": "UniRef90_A0A328EB30", "uniref50_id": "UniRef50_A0A328EB30" }, "pscc": { "uniref50_id": "UniRef50_A0A328EB30", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EB30" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 31001.693700000025, "isoelectric_point": 9.332975196838383 }, "id": "NKOCPLAKPK_2106", "locus": "GCA003260355_02102" }, { "type": "cds", "sequence": "contig_3", "start": 93909, "stop": 94211, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02421.24", "PFAM:PF00009.33" ], "nt": "ATGCTCGAGTTTATGAGACCGATTGTGATTTTTATGGTGGATTGTACGCAAGGAATCACGCATAGAGATATGACGCTTTTGCAGGAAATGACAATGTTGGGGCTTCCGATGATTTTTGCTCTCAATAAGGCTGATCTTGTAAAAAAGGAGGCGATTGATGCAATGGTAAAAGGTGCTCAGTCTTACCTAGATTTTGCAAAACATATTCCGATTATCCCACTTTCGGCGCTTAATGGGGACGGAATCGGGAATCTCCTTAGAATGGTGCAAAGAGTCCATCAAGAAAATCAGAAAAGAATCTGA", "aa": "MLEFMRPIVIFMVDCTQGITHRDMTLLQEMTMLGLPMIFALNKADLVKKEAIDAMVKGAQSYLDFAKHIPIIPLSALNGDGIGNLLRMVQRVHQENQKRI", "aa_hexdigest": "05029ee3e3df8a1660c7810415fe98f1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02421.24", "name": "FeoB_N", "length": 80, "aa_cov": 0.8, "hmm_cov": 0.925, "evalue": 9.645422019119351e-08, "score": 28.143423080444336, "start": 8, "stop": 87 }, { "id": "PF00009.33", "name": "GTP_EFTU", "length": 91, "aa_cov": 0.85, "hmm_cov": 1.0, "evalue": 1.9611285380303132e-13, "score": 46.8199348449707, "start": 8, "stop": 92 } ], "seq_stats": { "molecular_weight": 11338.47409999999, "isoelectric_point": 7.934398078918457 }, "id": "NKOCPLAKPK_2107", "locus": "GCA003260355_02103" }, { "type": "cds", "sequence": "contig_3", "start": 94302, "stop": 94529, "strand": "-", "frame": 2, "gene": null, "product": "G domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3D5YHJ6" ], "nt": "TTGGAGGATTCGATCAAGGAATTTGTAAGAGACTGGAAAGATAAACATCCAGATGTCCAGGAGCTAGATCTCCCTCAACAGCGTGGAATCGGAATCGCAATCGTCGGTAAGCCAAATTCTGGAAAATCTACGCTACTCAATACTTTAGTCGGACATGAACTCGCAAAGGTTGAGGATAAATTAGGGACGACAAGGGATTACCTCATTGGACAATTCAAGTCCCAGTGA", "aa": "MEDSIKEFVRDWKDKHPDVQELDLPQQRGIGIAIVGKPNSGKSTLLNTLVGHELAKVEDKLGTTRDYLIGQFKSQ", "aa_hexdigest": "5b146815b158b166f1b53257e5390e1b", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3D5YHJ6", "query_cov": 0.8933333333333333, "subject_cov": 0.9305555555555556, "identity": 0.537, "score": 64.7, "evalue": 2.9e-12, "valid": false, "product": "G domain-containing protein", "uniref50_id": "UniRef50_A0A3D5YHJ6" }, "pscc": { "uniref50_id": "UniRef50_A0A3D5YHJ6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3D5YHJ6" ], "product": "G domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_2108", "locus": "GCA003260355_02104" }, { "type": "cds", "sequence": "contig_3", "start": 94614, "stop": 94988, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00025.27", "PFAM:PF00350.29", "PFAM:PF02421.24", "PFAM:PF00009.33", "PFAM:PF04670.18", "PFAM:PF08477.19" ], "nt": "ATGAAAATAGGGCTTATCGGAGCAACAAACGCAGGAAAATCTACACTTTTCAACCACCTCATTGGGCAGTTTAGGGCAATAGTGACGGATATTCATGGTACGACAACCGATATCATCACGCATCAGATTGAGGTTGACGATCTAGGAAAGTTAACATTTTTTGATAGTCCAGGATTGCATGATTTCTCAGATGAGCTGCCTTTTATTGAAAAAATTGTGAAAGAATCAGATTTATTGCTTTTTGTAATTGATGATACGGTCGGAGTTTCGGCAAAGGACCAGCATATTCTTTCGCTCATTACGGAGAATGACAAGAGAAATCAGACTTTTCTTGTCGTGAATAAGCTAGATGTAAAGCGGAAAGCTAATGAATGA", "aa": "MKIGLIGATNAGKSTLFNHLIGQFRAIVTDIHGTTTDIITHQIEVDDLGKLTFFDSPGLHDFSDELPFIEKIVKESDLLLFVIDDTVGVSAKDQHILSLITENDKRNQTFLVVNKLDVKRKANE", "aa_hexdigest": "dc95c483660a0ddffabe4f6c8e6b035b", "start_type": "ATG", "rbs_motif": "TTATT", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00025.27", "name": "Arf", "length": 125, "aa_cov": 0.9838709677419355, "hmm_cov": 0.936, "evalue": 1.611359100941945e-06, "score": 24.15145492553711, "start": 1, "stop": 122 }, { "id": "PF00350.29", "name": "Dynamin_N", "length": 86, "aa_cov": 0.6774193548387096, "hmm_cov": 0.9767441860465116, "evalue": 4.1143674133367074e-12, "score": 42.88758087158203, "start": 32, "stop": 115 }, { "id": "PF02421.24", "name": "FeoB_N", "length": 126, "aa_cov": 0.967741935483871, "hmm_cov": 0.9444444444444444, "evalue": 2.5864334378278435e-10, "score": 36.497623443603516, "start": 2, "stop": 121 }, { "id": "PF00009.33", "name": "GTP_EFTU", "length": 136, "aa_cov": 0.9516129032258065, "hmm_cov": 0.9558823529411765, "evalue": 6.49318318340784e-11, "score": 38.602630615234375, "start": 2, "stop": 119 }, { "id": "PF04670.18", "name": "Gtr1_RagA", "length": 124, "aa_cov": 0.9516129032258065, "hmm_cov": 1.0, "evalue": 5.568276948718651e-07, "score": 25.650169372558594, "start": 2, "stop": 119 }, { "id": "PF08477.19", "name": "Roc", "length": 124, "aa_cov": 0.9354838709677419, "hmm_cov": 0.9596774193548387, "evalue": 1.452175920613346e-07, "score": 28.18729591369629, "start": 2, "stop": 117 } ], "seq_stats": { "molecular_weight": 13818.60209999998, "isoelectric_point": 5.2556970596313475 }, "id": "NKOCPLAKPK_2109", "locus": "GCA003260355_02105" }, { "type": "cds", "sequence": "contig_3", "start": 95366, "stop": 95647, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATCGTTATTTTTGTATTTTCATCGCTATACACCTCCGCAAATAGTATTGGCAATGGAGAACAAACAACAGAACTGCTTATCGGATGGGGGAATCTCCTTCAGGTCGTCGCTAATGTAGCATACGTTATTCTCCGACCATTGGTAGCACTTGCTGGATTGGCGATGGATAATCAGCTTATCTATGGTTCTTTTATGGGATTAGATGCTTCGCTTTGGCAGATTTGGCAGATTGTAAGGACTTTTTCAAACTATGCACTTGGATTGATATTTTTGATCTGA", "aa": "MIVIFVFSSLYTSANSIGNGEQTTELLIGWGNLLQVVANVAYVILRPLVALAGLAMDNQLIYGSFMGLDASLWQIWQIVRTFSNYALGLIFLI", "aa_hexdigest": "fa3d51fd86808aeb503f36caad790bb3", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10242.924099999997, "isoelectric_point": 4.316091728210449 }, "id": "NKOCPLAKPK_2110", "locus": "GCA003260355_02106" }, { "type": "cds", "sequence": "contig_3", "start": 95753, "stop": 96085, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCTAATCCAAGCAACATGGTTTATTATGATGGTGCTGGTAGATCTTTCGACAATTCTGACGTATTCTGTAGGAGGGATCCCGACGAGCATTATTACACAACTTGATGAAAAAGTCCATGATGTGAGGTTGCTTTCCACTAATGTCCTACTTAACCTTGGAGATGAGGGGCTTCAGAAAGATAAAACCGTCGATCAAGCGATTGTTACCTATTGGACTACGAAACAAAACAACAAAGATACAAACTATATCGCTCCGTGTAAAATTGCAGAAGTTCAAAAACAAAAGTTTATCGTAGGAAGGGCGTTTGATTCACTTACAGGAAGCAATTGA", "aa": "MLIQATWFIMMVLVDLSTILTYSVGGIPTSIITQLDEKVHDVRLLSTNVLLNLGDEGLQKDKTVDQAIVTYWTTKQNNKDTNYIAPCKIAEVQKQKFIVGRAFDSLTGSN", "aa_hexdigest": "f52317f001b2312835a6e66e3912fc9e", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12291.072799999985, "isoelectric_point": 5.663687324523925 }, "id": "NKOCPLAKPK_2111", "locus": "GCA003260355_02107" }, { "type": "cds", "sequence": "contig_3", "start": 96472, "stop": 97401, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAAAGGTTGAAATCGGATCTGCTCCTTCAGTAAAAAAGGCCTTACTTTTTTCCTTAAATGCTTTATCATCATTGCCTTTATCATTATCGTCGGGAGAAAGCCAGTCCTTGAGATTCTTATCACTTTCTTCTTGTAGTTTATTAGCGAATTTATTAGCTATAGAGTCTCCTCCAACAGAGGCATTTTTCAGAGTTTGTAGCGAAAGTCATTTGCTACCAATTGGAATAAAAGGAGTACTCATAAAGGTCTTTTGTCAAAGCTCTTGCAGAGAGGATCAAATTGCTTTCCCAACAGAAGTTTGCTTGATAGCCCAAAAGAGTAGGAACCACGTAATTCCAAGCCCAAAGAGCATAATAACCACCCAAGAAAGGTTGAGGAGCCCATTATTGAGGTGTATCTTGATAAATCCAAGAATTCAGAGTGATTCATCTTTAGGATCGACCTCAAATCCAGAGATATCTTTGAGTACTTTTGCAGTACTAACACCGCCATTGGAGGGACCAGAAGCTTCATTACCTAAGCTGCTTTTAAGCGCACTCATAAAAACTAAAGAAATGCTGACTGCAAAAGAGATTAAGACAGGAGCGATAAGGAGCTTCATGAGTCAATCAATAGAAAGCCATTCAGGAAGAGCCTTCCCACCAAAAATTTTCAATAATGCAATAAACGGAGATAGAGCTATTGCTATCCAGAGAATCCCGACTCTCGCAAAAAGCACAACTACCAGAGCTATCAATGGTAAGATTAAAAGCACAGAAAATCCTGTATTGATCAGTGCGAGAATAAAGAGTTGCCATACTCACAAATCCTTGTATCCCGAAAGATCAGCAAAATTAAACATATTTGAATACAGAGAAACAAAAGGTCAAGTCATCGATTTTCCCTTATTGATGATGGTAGAAAGGGAAATGTCTGTTTTGTCATAG", "aa": "MLKVEIGSAPSVKKALLFSLNALSSLPLSLSSGESQSLRFLSLSSCSLLANLLAIESPPTEAFFRVCSESHLLPIGIKGVLIKVFCQSSCREDQIAFPTEVCLIAQKSRNHVIPSPKSIITTQERLRSPLLRCILINPRIQSDSSLGSTSNPEISLSTFAVLTPPLEGPEASLPKLLLSALIKTKEMLTAKEIKTGAIRSFMSQSIESHSGRAFPPKIFNNAINGDRAIAIQRIPTLAKSTTTRAINGKIKSTENPVLISARIKSCHTHKSLYPERSAKLNIFEYRETKGQVIDFPLLMMVEREMSVLS", "aa_hexdigest": "90b8e8ecc84959e15ff1a4d9e1cf8e6a", "start_type": "ATG", "rbs_motif": "TTTA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 33769.15460000004, "isoelectric_point": 9.605934333801269 }, "id": "NKOCPLAKPK_2112", "locus": "GCA003260355_02108" }, { "type": "cds", "sequence": "contig_3", "start": 97604, "stop": 97744, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAGCAATTCTGAGTGACGGTACTATACAGAAATCTGGTAGAATCGGAGAATGGAGTAAAAATGCAAATATCAAGATTGGAAATAAGCAATACTCCCTTGATGCGACCAGCGGAAAATTAACAGTGAAAGGAAAGTAA", "aa": "MEAILSDGTIQKSGRIGEWSKNANIKIGNKQYSLDATSGKLTVKGK", "aa_hexdigest": "52346154d99f94eea9ae893090d53290", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4937.586700000002, "isoelectric_point": 9.777162361145017 }, "id": "NKOCPLAKPK_2113", "locus": "GCA003260355_02109" }, { "type": "cds", "sequence": "contig_3", "start": 97820, "stop": 98074, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATCAGTTTTTTCCTCGTTGGGAATGCTTTTGCGGCGGACATTTGTACTACTGAAACGACAAAAGGATTGGAAACCTTTAAAACTATTCTGACCGTCATAGGAAGTTTTGCTTCTTGGGTATGGGTTATTCTGGGTAGTATTGCAGGGAAATTGATGACCAATACGTTTGTTTATGGAGAGTTTATACATCTCGATGGACTTCTCTGGAAAATCCGACAAATCTCAAGAACTTTTGTAAACTATACAATCTGA", "aa": "MISFFLVGNAFAADICTTETTKGLETFKTILTVIGSFASWVWVILGSIAGKLMTNTFVYGEFIHLDGLLWKIRQISRTFVNYTI", "aa_hexdigest": "7d2005da8b6e0a1a1cc1d376bdb2ee2a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9429.971199999994, "isoelectric_point": 7.907708168029785 }, "id": "NKOCPLAKPK_2114", "locus": "GCA003260355_02110" }, { "type": "cds", "sequence": "contig_3", "start": 98084, "stop": 98914, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MSM1", "UniRef:UniRef90_A0A660MSM1" ], "nt": "TTGATCGGTATTTTTAACTATATTTTTAATTTTAGCACTAAGGCAAAGAATCCTACCTCGTTCATTACTGAACTTTTGATAGCTAGTATATTGGTCCAAGCGAGCCGATTCCTTGTGATGGTGCTTCTTGACTTTTCTACAATAGGGATAGCAACGATTTCTTCTTTCCCATCACAAGTCATTAGTTCCAACTCAAATTATCGTGAATCTCTTCTTACCTCCATCAAAAGCGATCCTCTTCTTTCCAAACCGTTTAAGACGCCACCTAAGGGGGTTGAAATTAACGTATTTGCGGATGTATTTTCTCAAAAAGATAAAGAACTCCTCTGGAAAGAATATGATGTGGAAAAATTACCTGATGATTTTGAGAAAAACTTAATTGACTCGCTACTACCTAAATCAGACAATCTCGGAGGCCCCCTTGTCTATCTTGGATTCTCAGTCTTTCAAAGCCAAAATCAGATCATAAACTATGAAGCTAATCCTACTAATTGTATCGAGATGTTTTCAAAAGCAATTATCAGTCTTATTCTGAGCTCAGGAATGATTATCCTCTATGCATTTTCGCTTTTGGTTTTGGTTGCTGTGCTTATTATTAGACTGCTTTATCTGCGGATTTTTATTGCGGCAAGTCCTATTATTGTCCTACTGTCTAGAAAAAAATTAATAGAAGATTTTTTGGGATTAAAATTGAACTTATCTACACCTGCTCTTAATCTTGGAGATATGATTGTTCTTGTTTTTAAACCTGTATTTTTCGCATTATGGATCAGTATTATGATTCTTTTTGTTGTCATTCTTCAAGGGTTCTTTTTGTCACAAGATAACTGA", "aa": "MIGIFNYIFNFSTKAKNPTSFITELLIASILVQASRFLVMVLLDFSTIGIATISSFPSQVISSNSNYRESLLTSIKSDPLLSKPFKTPPKGVEINVFADVFSQKDKELLWKEYDVEKLPDDFEKNLIDSLLPKSDNLGGPLVYLGFSVFQSQNQIINYEANPTNCIEMFSKAIISLILSSGMIILYAFSLLVLVAVLIIRLLYLRIFIAASPIIVLLSRKKLIEDFLGLKLNLSTPALNLGDMIVLVFKPVFFALWISIMILFVVILQGFFLSQDN", "aa_hexdigest": "d415255fc3dc9213cf099cb3a0e5a8a8", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 97784, "stop": 99217, "observations": { "mutation": [ 97805, 98072, 98912, 99011, 99140, 99143 ], "stop": [ 97805, 98072, 98912, 99011, 99140, 99143 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A660MSM1", "query_cov": 1.0, "subject_cov": 0.4188163884673748, "identity": 0.996, "score": 494.0, "evalue": 5.52e-170, "gene_start": 1, "gene_end": 276, "reference_start": 106, "reference_end": 381, "reference_sequence": "MPRRKQISQRKIGIILVMISVFLVGNAFAADICTTETTKGLETFKTILTVIGSFASWVWVILGSIAGKLMTNTFVYGEFIHLDGLLRKIRQISRTFVNYTIGILFLIGIFNYIFNFSTKAKNPTSFITELLIASILVQASRFLVMVLLDFSTIGIATISSFPSQVISSNSNYRESLLTSIKSDPLLSKPFKTPPKGVEINVFADVFSQKDKELLWKEYDVEKLPDDFEKNLIDSLLPKSDNLGGPLVYLGFSVFQSQNQIINYEANPTNCIEMFSKAIISLILSSGMIILYAFSLLVLVAVLIIRLLYLRIFIAASPIIVLLSRKKLIEDFLGLKLNLSTPALNLGDMIVLVFKPVFFALWISIMILFVVILQGFFLSQDNGSSEFDNGSVKVSETITSDQARSINSYQSELTIGEHAKVALYHGTKSFKDIMLAFMTLALMWYFVKLAFSSGKTKIGGIDKLTSGVMETAEKVVGNIGIIPTPAGQIGLKQAKDIPSTLIKNKQGQLEAEETERLKAFYRDIGIETGALSMSESQKTKLKEQANATNLESYPSLILSIKNQNNGIKFNDIPVSSLHNWIQNVNKHSSKDQIFSKYFGPNWQDIPNIKLETGDKAEEKYLKELFKSATRVQTFYQKMLGGKNNIFTYEGLISDRGIVKN" }, "paralog": false, "identity": 0.9790356394129979, "score": 885.0, "evalue": 0.0, "description": "Internal stop codon at 97805, 98072, 98912, 99011, 99140, 99143. Nonsense mutation around 97805, 98072, 98912, 99011, 99140, 99143.", "psc": { "uniref90_id": "UniRef90_A0A660MSM1", "uniref50_id": "UniRef50_A0A660MSM1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A660MSM1", "UniRef:UniRef50_A0A660MSM1" ] }, "pscc": { "uniref50_id": "UniRef50_A0A660MSM1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MSM1" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 30939.326800000043, "isoelectric_point": 6.343936347961425 }, "id": "NKOCPLAKPK_2115", "locus": "GCA003260355_02111" }, { "type": "cds", "sequence": "contig_3", "start": 99170, "stop": 99604, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAACTGCAGAAAAAGTCGTTGGAAATGTAGGGATTATCCCAACACCAGCTGGTCAGATTGGATTAAAACAGGCAAAAGATATTCCTAGCACACTTATAGGAAATAAGCAGGGACAACTAGAGGCTGAAGAAAGAGAAAGATTAAACGCATTTTACAGAGACATTGGAATAGATCCTGGAGCTCTTTCAATGAGTGAATCTCAAAAAACCAAACTCAAGGAACAGGCTAATGCAACTAATCTTGAGAGTTATCCATCTCTAATCTTATCAATAAAGAATCAAAATAACGGGATTAAATTTAATGATATCCCGGTTTCTTCTTTGTATAATTGGATTCAGAATGTAAATAAGCATAGTTCTAAAGATCAGATTTTTAGTAAGTATTTTGGTCCAAACTGGCAAGATATCCCAAATATTAAATTAGAAACCTGA", "aa": "METAEKVVGNVGIIPTPAGQIGLKQAKDIPSTLIGNKQGQLEAEERERLNAFYRDIGIDPGALSMSESQKTKLKEQANATNLESYPSLILSIKNQNNGIKFNDIPVSSLYNWIQNVNKHSSKDQIFSKYFGPNWQDIPNIKLET", "aa_hexdigest": "ce21336f0032940a6240c88b887dc3a9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16031.905599999987, "isoelectric_point": 6.590447044372559 }, "id": "NKOCPLAKPK_2116", "locus": "GCA003260355_02112" }, { "type": "cds", "sequence": "contig_3", "start": 100120, "stop": 100278, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MNF4", "UniRef:UniRef90_A0A660MNF4" ], "nt": "ATGCCCAAGATGCTTCCCGCACTGCCTAATAACCGTCTTATTCTCAATGGACATAAAATTCTGAACCAAGATCAGCTTTTTGCCTCTATTAGTAAGAGGCTCAAGACCACTGTTCTTAACAGACAAGAGCTTCGCAAACTTCTCAGTAGCTATCAGTGA", "aa": "MPKMLPALPNNRLILNGHKILNQDQLFASISKRLKTTVLNRQELRKLLSSYQ", "aa_hexdigest": "959a89cf9f69893e6d3403cc8e152a8b", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 100129, "stop": 100404, "observations": { "mutation": [ 100276 ], "stop": [ 100276 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A660MNF4", "query_cov": 0.9423076923076923, "subject_cov": 0.5384615384615384, "identity": 0.9179999999999999, "score": 87.4, "evalue": 1.94e-21, "gene_start": 4, "gene_end": 52, "reference_start": 1, "reference_end": 49, "reference_sequence": "MLPALPNNRLILNGHKIPNQDQLFTFISKRLKTTVLDRQELRKLLSSYQGLHIAIWHGDSFLEEENEATQKEIVLLLSELGTLKISGKKKR" }, "paralog": false, "identity": 0.9340659340659341, "score": 165.0, "evalue": 9.56e-55, "description": "Internal stop codon at 100276. Nonsense mutation around 100276.", "psc": { "uniref90_id": "UniRef90_A0A660MNF4", "uniref50_id": "UniRef50_A0A660MNF4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A660MNF4", "UniRef:UniRef50_A0A660MNF4" ] }, "pscc": { "uniref50_id": "UniRef50_A0A660MNF4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A660MNF4" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 6045.136199999999, "isoelectric_point": 11.096959114074707 }, "id": "NKOCPLAKPK_2117", "locus": "GCA003260355_02113" }, { "type": "cds", "sequence": "contig_3", "start": 100538, "stop": 100696, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATCCTAAGGATTATGCTGATATTTTTCCTTATGCAGAAGCATTTAAAGCGATCCGCAGAAAAATTGGTCAGGATTTTTCTCTTCGTAAGATTGGTGAGGGGGCTTGGGCGAGTGTATTTGAAATCCAAAAAGACGGGAAATGAGTTTATGTGGTAA", "aa": "MILRIMLIFFLMQKHLKRSAEKLVRIFLFVRLVRGLGRVYLKSKKTGNEFMW", "aa_hexdigest": "360eb13af9442866b7530207c02082dc", "start_type": "ATG", "rbs_motif": "TTTA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6356.8616, "isoelectric_point": 11.73919315338135 }, "id": "NKOCPLAKPK_2118", "locus": "GCA003260355_02114" }, { "type": "cds", "sequence": "contig_3", "start": 100690, "stop": 100881, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGTAAAGTTACCTCATCCTGAATATCTAGATTCTCAAGATATTAATATCAAAGATAAAGTGAAAGCTGAAATAAAGCTCCAAAAAAAGTTCATTCTTGAATATGATACCTGGGTCAAGAAACTGCATAAAAACTATCTCAGTATTGTTACTGATCCAACAGGCTTTAACCTTTATTCTACACTTTCATGA", "aa": "MVKLPHPEYLDSQDINIKDKVKAEIKLQKKFILEYDTWVKKLHKNYLSIVTDPTGFNLYSTLS", "aa_hexdigest": "dc8092eae1456d7dd559e0f376b4c93b", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7439.5808, "isoelectric_point": 8.893171882629396 }, "id": "NKOCPLAKPK_2119", "locus": "GCA003260355_02115" }, { "type": "cds", "sequence": "contig_3", "start": 101263, "stop": 101376, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGGAATCTGATCCTACAATAATGATATCAAGTTTTATTAAAGAGTTTGATGGGGTCCGAGAGCCGGATCCTACAGTTTTTACACAGCTTTATGGGGATAGTAAGAAGTGA", "aa": "MLESDPTIMISSFIKEFDGVREPDPTVFTQLYGDSKK", "aa_hexdigest": "a28d8730cb3f8eb4b703de7557685aa4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4222.744400000001, "isoelectric_point": 4.385492134094237 }, "id": "NKOCPLAKPK_2120", "locus": "GCA003260355_02116" }, { "type": "cds", "sequence": "contig_3", "start": 101701, "stop": 101967, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EBY2", "UniRef:UniRef90_A0A328EBY2" ], "nt": "ATGAAGAATCACACAAGAACCTTTTTGTATTTTCACAATCTTGCTCAGCTGTCTGATGAAGTGATCTTTCCTTACCTTGAGCAACTCCAAAAAGATTATCCGAATCTCCAAGGCTCTGTTTATCTCAGGCATCATAAAGGAAAAAAATTTATCTCACTAGAACCCTTATTCCCTGATTCTGGAGAAAAGAAAATTGCTCAAAAACTAGCTGATGAGCTGAAATCTCTTTTGAAACAAAAAGATAAAAATCAGACTTCCACTGTTTAA", "aa": "MKNHTRTFLYFHNLAQLSDEVIFPYLEQLQKDYPNLQGSVYLRHHKGKKFISLEPLFPDSGEKKIAQKLADELKSLLKQKDKNQTSTV", "aa_hexdigest": "d43ea8f436709f5e2a71265cce379d82", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 101521, "stop": 101967, "observations": { "mutation": [ 101569, 101575, 101689 ], "stop": [ 101569, 101575, 101689 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EBY2", "query_cov": 1.0, "subject_cov": 0.5945945945945946, "identity": 0.966, "score": 168.0, "evalue": 4.95e-52, "gene_start": 1, "gene_end": 88, "reference_start": 61, "reference_end": 148, "reference_sequence": "MTLNNPKTTNQPPVQPGSGSNTSPTKENLKETLIPNLDTMRYMEMMTLFNSKTYDEGVVRMKNHTRTFLYFHNLAQLSDEEISPHLEQLQKDYPNLQGSVYLRHHKGKKFISLEPLFPDSGEKKIAQKLADELKSLLKQKDKNQTSTV" }, "paralog": false, "identity": 0.9459459459459459, "score": 279.0, "evalue": 3.77e-98, "description": "Internal stop codon at 101569, 101575, 101689. Nonsense mutation around 101569, 101575, 101689.", "psc": { "uniref90_id": "UniRef90_A0A328EBY2", "uniref50_id": "UniRef50_A0A328EBY2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EBY2", "UniRef:UniRef50_A0A328EBY2" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EBY2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EBY2" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 10318.733499999991, "isoelectric_point": 9.363404273986816 }, "id": "NKOCPLAKPK_2121", "locus": "GCA003260355_02117" }, { "type": "cds", "sequence": "contig_3", "start": 102048, "stop": 102614, "strand": "-", "frame": 2, "gene": "tgt", "product": "tRNA guanosine(34) transglycosylase Tgt", "db_xrefs": [ "EC:2.4.2.29", "GO:0005737", "GO:0006400", "GO:0008479", "SO:0001217", "UniRef:UniRef50_A0A288DG82", "UniRef:UniRef90_A0A328EFR6" ], "nt": "ATGGTGCTAGATGTTTGCTCTCCTACTGGTTCTGACAAGAAAACCATAGAAAAGCATATTGCTATGACCCATCGCCGAGCAAAAAGAGCTTTTGAACATTTCAAGCCCAAATACGAAGAGGCGAGAGGTGTCCTCTTCCCTATCGTGCAAGGGGGGACGTATCTCGACCTCAGAACCCAATCCGCAGAATACCTTTCTCAGTTCGCTCGGGATGGAATTGCGATCGGTGGGGTGAGTGTTGGAGAAAGTAGAGAGCTGATTGAGCAAGTGATTGCACATACTGCACCACTTTTGCCAAATGAGAAGCCGAGATATTTGATGGGCGTAGGAACTCCAGAAGACCTGCGTTTTGCGATAGAGCAGGGAGTAGATATGTTTGACTGCGTCGCAGCAACGAGACTTGGAAGGCACGGAATCGCCTTTTCTGACCAAGGGAATATCAAAATAACCAATTCTGACTTTAGAACTGATTTCTCTCCTTTGACTGAGAACTGCGATTGCTACACTTGCAGAAATTTTAGCAAAGCTTACCTCTGCCACCTCATCAGAGAACAGGAAACACTCTGA", "aa": "MVLDVCSPTGSDKKTIEKHIAMTHRRAKRAFEHFKPKYEEARGVLFPIVQGGTYLDLRTQSAEYLSQFARDGIAIGGVSVGESRELIEQVIAHTAPLLPNEKPRYLMGVGTPEDLRFAIEQGVDMFDCVAATRLGRHGIAFSDQGNIKITNSDFRTDFSPLTENCDCYTCRNFSKAYLCHLIREQETL", "aa_hexdigest": "814170a6eb719fb8e82b4a16b197fe14", "start_type": "ATG", "rbs_motif": null, "genes": [ "tgt" ], "truncated": "both", "pseudogene": { "start": 101964, "stop": 102914, "observations": { "mutation": [ 102050, 102629 ], "stop": [ 102050, 102629 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EFR6", "query_cov": 1.0, "subject_cov": 0.49343832020997375, "identity": 0.9840000000000001, "score": 378.0, "evalue": 8.23e-130, "gene_start": 1, "gene_end": 188, "reference_start": 166, "reference_end": 353, "reference_sequence": "MSKLNFKILKQKGKARVGEITLNGITLKTPIFMPVGTKATIKGLILDLLQDSHYIGNQIEPIKLILANTFHLYLRPGSKIVQAAGGLHQFENWKDGLILTDSGGFQVFSLGLANQKFNDQKHTHKVGIKLTEEGVKFRSPYDGSKHIFTPENVVDTQCELGSDIMMVLDVCSPAGSDKKTIEKHIAMTHRRAKRAFEHFKPKYEESRGVLFPIVQGGTYLDLRTQSAEYLSQFAWDGIAIGGVSVGESRELIEQVIAHTAPLLPNEKPRYLMGVGTPEDLRFAIEQGVDMFDCVAATRLGRHGIAFSDQGNIKITNSDFRTDFSPLTENCDCYTCRNFSKAYLCHLIREQETLGGSLVGLHNIVYLNRILEDWKKKFLEEN" }, "paralog": false, "identity": 0.9746835443037974, "score": 625.0, "evalue": 1.65e-229, "description": "Internal stop codon at 102050, 102629. Nonsense mutation around 102050, 102629.", "psc": { "uniref90_id": "UniRef90_A0A328EFR6", "gene": "tgt", "product": "tRNA guanosine(34) transglycosylase Tgt", "ec_ids": [ "2.4.2.29" ], "uniref50_id": "UniRef50_A0A288DG82", "go_ids": [ "GO:0005737", "GO:0006400", "GO:0008479" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EFR6", "EC:2.4.2.29", "UniRef:UniRef50_A0A288DG82", "GO:0005737", "GO:0006400", "GO:0008479" ] }, "pscc": { "uniref50_id": "UniRef50_A0A288DG82", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A288DG82" ], "product": "Queuine tRNA-ribosyltransferase" } }, "id": "NKOCPLAKPK_2122", "locus": "GCA003260355_02118" }, { "type": "cds", "sequence": "contig_3", "start": 102627, "stop": 103109, "strand": "-", "frame": 2, "gene": "tgt", "product": "tRNA guanosine(34) transglycosylase Tgt", "db_xrefs": [ "EC:2.4.2.29", "GO:0005737", "GO:0006400", "GO:0008479", "SO:0001217", "UniRef:UniRef50_A0A288DG82", "UniRef:UniRef90_A0A328EFR6" ], "nt": "ATGTCAAAACTCAACTTCAAAATCCTCAAGCAAAAAGGGAAAGCCAGAGTCGGTGAAATCACCCTCAATGGAATCAGCCTCAAAACACCGATTTTTATGCCGGTGGGGACCAAAGCCACGATCAAAGGTCTGATTTTAGATCTTTTGCAGGATTCACACTATATTGGAAATCAAATTGAGCCGATCAAGCTCATTCTCGCAAACACTTTTCATCTCTATCTTCGTCCGGGAAGTAAAATTGTCCAGGCGGCTGGAGGTTTGCATCAGTTTGAAAACTGGAAAGACGGACTGATTCTGACTGATAGCGGAGGGTTTCAGGTTTTTTCTCTTGGTCTAGCAAATCAAAAATTCAACGACCAAAAACATACTCACAAAGTAGGAATAAAGCTGACTGAAGAGGGTGTAAAATTCCGCTCGCCTTATGATGGATCAAAGCATATCTTCACCCCAGAAAATGTGGTAGATACCCAATGTGAGCTTTGA", "aa": "MSKLNFKILKQKGKARVGEITLNGISLKTPIFMPVGTKATIKGLILDLLQDSHYIGNQIEPIKLILANTFHLYLRPGSKIVQAAGGLHQFENWKDGLILTDSGGFQVFSLGLANQKFNDQKHTHKVGIKLTEEGVKFRSPYDGSKHIFTPENVVDTQCEL", "aa_hexdigest": "830fe5d5fdc5ebb7aad3e72f10f5acd8", "start_type": "ATG", "rbs_motif": null, "genes": [ "tgt" ], "truncated": "5-prime", "pseudogene": { "start": 102324, "stop": 103109, "observations": { "mutation": [ 102629 ], "stop": [ 102629 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EFR6", "query_cov": 1.0, "subject_cov": 0.4199475065616798, "identity": 0.9940000000000001, "score": 323.0, "evalue": 1.36e-108, "gene_start": 1, "gene_end": 160, "reference_start": 1, "reference_end": 160, "reference_sequence": "MSKLNFKILKQKGKARVGEITLNGITLKTPIFMPVGTKATIKGLILDLLQDSHYIGNQIEPIKLILANTFHLYLRPGSKIVQAAGGLHQFENWKDGLILTDSGGFQVFSLGLANQKFNDQKHTHKVGIKLTEEGVKFRSPYDGSKHIFTPENVVDTQCELGSDIMMVLDVCSPAGSDKKTIEKHIAMTHRRAKRAFEHFKPKYEESRGVLFPIVQGGTYLDLRTQSAEYLSQFAWDGIAIGGVSVGESRELIEQVIAHTAPLLPNEKPRYLMGVGTPEDLRFAIEQGVDMFDCVAATRLGRHGIAFSDQGNIKITNSDFRTDFSPLTENCDCYTCRNFSKAYLCHLIREQETLGGSLVGLHNIVYLNRILEDWKKKFLEEN" }, "paralog": false, "identity": 0.9808429118773946, "score": 512.0, "evalue": 2.16e-185, "description": "Internal stop codon at 102629. Nonsense mutation around 102629.", "psc": { "uniref90_id": "UniRef90_A0A328EFR6", "gene": "tgt", "product": "tRNA guanosine(34) transglycosylase Tgt", "ec_ids": [ "2.4.2.29" ], "uniref50_id": "UniRef50_A0A288DG82", "go_ids": [ "GO:0005737", "GO:0006400", "GO:0008479" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EFR6", "EC:2.4.2.29", "UniRef:UniRef50_A0A288DG82", "GO:0005737", "GO:0006400", "GO:0008479" ] }, "pscc": { "uniref50_id": "UniRef50_A0A288DG82", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A288DG82" ], "product": "Queuine tRNA-ribosyltransferase" } }, "id": "NKOCPLAKPK_2123", "locus": "GCA003260355_02119" }, { "type": "cds", "sequence": "contig_3", "start": 103146, "stop": 104012, "strand": "-", "frame": 2, "gene": "thyA", "product": "thymidylate synthase", "db_xrefs": [ "EC:2.1.1.45", "GO:0004799", "GO:0006231", "KEGG:K00560", "SO:0001217", "UniParc:UPI000DBFA4CB", "UniRef:UniRef100_A0A328EBS0", "UniRef:UniRef50_A0A6S4QFH0", "UniRef:UniRef90_A0A328EBS0" ], "nt": "ATGCAAAGTTATCTCGCCATTGTCAAAAACGTTCTAGACCACGGAGAACGAAAAGACAACAGGACCGGAATCCGCACAAAAGCCATCGCAGGAACCTTTTTTGAGCACAATATGCAGGAGGGCTTTCCTCTTCTGACCACCAAAAAAATCCCTTTTAGACTTGTGGCTTCCGAATTAGAATTTTTCATCAAGGGAATTACTGACAAGGAGCGGCTTCTAGCGAGAAATAACCATATTCGAGACGAATGGTGCTCGCCTGACCGTGTGCCTTATAGTCATGATACTGCGACGCAGGCAGCGATGAAAGCGGAGAGGGAACTTGGACCGATTTATGGCTTTCAGCGGAGGCATTTCGGTGCTAAGTATCAGACCTTTAGTACTAATTACGATCAGCAGGGTGTAGATCAGTTAAAAAATCTGATCCAAACCCTCAAAAGGAACCCTTCCGACAGGAGAATGATCGTCTCAGCCTGGAATCCAATGGATCTAGGATGTATGGCTTTACCGCCTTGTCATTATGGATTTCAGGTGACCGTCATCAATGGAAAACTCAACCTCCTGCGAAATCAAAGATCAGTAGACATCGGATTAGGACTCCCATTCAATATCGCAAGCTATGGGCTTTTACTCCATCTTCTTGCCAAGGAAGCAGGACTACAAGAAGGGAAGCTGGTTGGATTTCTCGCGGATACCCATATTTATGAGAACCATATTTCTGTTCTTCAGGAACAGCTGACTAGAATACCAAAGCCCCTTCCAAATATAGAAACCAAGCAATGCTCTTCAATTTTTGAACGGGAATACACCGACACCGTAATAAACGGCTATGATCCAGATGCTTCTTTGCCTATGGATGTCGCTGTCTAA", "aa": "MQSYLAIVKNVLDHGERKDNRTGIRTKAIAGTFFEHNMQEGFPLLTTKKIPFRLVASELEFFIKGITDKERLLARNNHIRDEWCSPDRVPYSHDTATQAAMKAERELGPIYGFQRRHFGAKYQTFSTNYDQQGVDQLKNLIQTLKRNPSDRRMIVSAWNPMDLGCMALPPCHYGFQVTVINGKLNLLRNQRSVDIGLGLPFNIASYGLLLHLLAKEAGLQEGKLVGFLADTHIYENHISVLQEQLTRIPKPLPNIETKQCSSIFEREYTDTVINGYDPDASLPMDVAV", "aa_hexdigest": "7c1c131c9d4b8d5bd50bea16c03e0b1d", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000DBFA4CB", "uniref100_id": "UniRef100_A0A328EBS0", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBFA4CB", "UniRef:UniRef100_A0A328EBS0" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EBS0", "uniref90_id": "UniRef90_A0A328EBS0" }, "psc": { "uniref90_id": "UniRef90_A0A328EBS0", "gene": "thyA", "product": "thymidylate synthase", "ec_ids": [ "2.1.1.45" ], "uniref50_id": "UniRef50_A0A6S4QFH0", "kegg_orthology_id": "K00560", "go_ids": [ "GO:0004799", "GO:0006231" ] }, "pscc": { "uniref50_id": "UniRef50_A0A6S4QFH0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A6S4QFH0" ], "product": "Thymidylate synthase" }, "genes": [ "thyA" ], "id": "NKOCPLAKPK_2124", "locus": "GCA003260355_02120" }, { "type": "cds", "sequence": "contig_3", "start": 104038, "stop": 104499, "strand": "-", "frame": 1, "gene": null, "product": "Dihydrofolate reductase", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBF960E", "UniRef:UniRef100_A0A328EF63", "UniRef:UniRef50_A0A2W4ILU9", "UniRef:UniRef90_A0A328EF63" ], "nt": "ATGAAGATTGCACTTATCGCAGCCATGGCTCCCAATAGGGTAATTGGATGCCAAAACCAGCTCCCTCGGCATTATTCTGAAGATCTTCAATATTTCAAAAAGACAACCCTGAATCATACTGTCGTTATGGGAAAGAATACCTTTATTTCACTGGGGAAACCGCTCCCGCAGAGGAGAAATGTTGTTATTACTTCCCAGCCGATTCCCTCCCTTGAATGTTATCCAGACAGGGCAAGCTTTTTGGCAGATTTTAAGCCCACAGATCCAGAAGAACAGGTTTTCATTATTGGAGGAGCTTATACCTATCAGAGCTTTCTTCCGGATGCAGATCTTATTTATCTGACCAAAATCAGAAAAAACTACCAAGGAGATACCTTTTTCCCCAGTTTTGAGTCAGATTTTGTCTGTATTTCAGAGACAACAGGACAAGAGGTAGACTTCCTAATCTATCAAAGAAAATAA", "aa": "MKIALIAAMAPNRVIGCQNQLPRHYSEDLQYFKKTTLNHTVVMGKNTFISLGKPLPQRRNVVITSQPIPSLECYPDRASFLADFKPTDPEEQVFIIGGAYTYQSFLPDADLIYLTKIRKNYQGDTFFPSFESDFVCISETTGQEVDFLIYQRK", "aa_hexdigest": "6eded893fbf94a99b9baf23735f9525e", "start_type": "ATG", "rbs_motif": "TTATT", "ups": { "uniparc_id": "UPI000DBF960E", "uniref100_id": "UniRef100_A0A328EF63", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DBF960E", "UniRef:UniRef100_A0A328EF63" ] }, "ips": { "uniref100_id": "UniRef100_A0A328EF63", "uniref90_id": "UniRef90_A0A328EF63" }, "psc": { "uniref90_id": "UniRef90_A0A328EF63", "product": "Dihydrofolate reductase", "uniref50_id": "UniRef50_A0A2W4ILU9" }, "pscc": { "uniref50_id": "UniRef50_A0A2W4ILU9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W4ILU9" ], "product": "Dihydrofolate reductase" }, "genes": [], "id": "NKOCPLAKPK_2125", "locus": "GCA003260355_02121" }, { "type": "cds", "sequence": "contig_3", "start": 104556, "stop": 104747, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EB36", "UniRef:UniRef90_A0A328EB36" ], "nt": "ATGATTTTTCTCTTGTGCAAGAACGATCCAGGAAAGCGAGAAGTCTCCATCAAACAGTGTGGACAGAAGGGAAAGGTAGACAAAGCAGACACAAAAAAGAAAAAATCCAATAAAGCTGAAGAAAACAGGGGATTCTACCAGAAAGAAGCACGATACGATTTTCAAATCCAACAAAAAATTTCTTTAGATTGA", "aa": "MIFLLCKNDPGKREVSIKQCGQKGKVDKADTKKKKSNKAEENRGFYQKEARYDFQIQQKISLD", "aa_hexdigest": "2589a673dffb25d9303c53d64981a001", "start_type": "ATG", "rbs_motif": "TTTA", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 104457, "stop": 104747, "observations": { "mutation": [ 104498, 104501, 104558 ], "stop": [ 104498, 104501, 104558 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A328EB36", "query_cov": 1.0, "subject_cov": 0.65625, "identity": 1.0, "score": 124.0, "evalue": 8.3e-36, "gene_start": 1, "gene_end": 63, "reference_start": 1, "reference_end": 63, "reference_sequence": "MIFLLCKNDPGKREVSIKQCGQKGKVDKADTKKKKSNKAEENRGFYQKEARYDFQIQQKISLDGKVGVKSYTEIRILFLLGSGGRLHLSQPWLPIG" }, "paralog": false, "identity": 0.96875, "score": 186.0, "evalue": 1.07e-62, "description": "Internal stop codon at 104498, 104501, 104558. Nonsense mutation around 104498, 104501, 104558.", "psc": { "uniref90_id": "UniRef90_A0A328EB36", "uniref50_id": "UniRef50_A0A328EB36", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A328EB36", "UniRef:UniRef50_A0A328EB36" ] }, "pscc": { "uniref50_id": "UniRef50_A0A328EB36", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A328EB36" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 7352.393000000002, "isoelectric_point": 9.615411186218264 }, "id": "NKOCPLAKPK_2126", "locus": "GCA003260355_02122" }, { "type": "cds", "sequence": "contig_3", "start": 105099, "stop": 105233, "strand": "+", "frame": 3, "gene": "secG", "product": "preprotein translocase subunit SecG", "db_xrefs": [ "GO:0015136", "GO:0015739", "GO:0016020", "SO:0001217", "UniRef:UniRef50_A0A7W4E3H3", "UniRef:UniRef90_A0A660MPM6" ], "nt": "ATGAGAACTTTATTAATCATTGCCATGCTCATTACTGGAATTGTCTTTATAGCAAGTGTTTTGCTTCAGTCTCCAAAAGGAGGTGGTATTGGAATGGGGCTCGGTGGAGCTTCTGCCGGAGGAAATGAATACTGA", "aa": "MRTLLIIAMLITGIVFIASVLLQSPKGGGIGMGLGGASAGGNEY", "aa_hexdigest": "58a313ef2e861e041af1a36b3734fb2e", "start_type": "ATG", "rbs_motif": null, "genes": [ "secG" ], "truncated": "3-prime", "pseudogene": { "start": 105099, "stop": 105329, "observations": { "mutation": [ 105231 ], "stop": [ 105231 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A660MPM6", "query_cov": 1.0, "subject_cov": 0.5789473684210527, "identity": 1.0, "score": 78.2, "evalue": 4.18e-18, "gene_start": 1, "gene_end": 44, "reference_start": 1, "reference_end": 44, "reference_sequence": "MRTLLIIAMLITGIVFIASVLLQSPKGGGIGMGLGGASAGGNEYGSKKTMEGKLKKIALISGVLFVIIVLFLPYVR" }, "paralog": false, "identity": 0.9868421052631579, "score": 142.0, "evalue": 5.54e-46, "description": "Internal stop codon at 105231. Nonsense mutation around 105231.", "psc": { "uniref90_id": "UniRef90_A0A660MPM6", "gene": "secG", "product": "preprotein translocase subunit SecG", "uniref50_id": "UniRef50_A0A7W4E3H3", "go_ids": [ "GO:0015136", "GO:0015739", "GO:0016020" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A660MPM6", "UniRef:UniRef50_A0A7W4E3H3", "GO:0015136", "GO:0015739", "GO:0016020" ] }, "pscc": { "uniref50_id": "UniRef50_A0A7W4E3H3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7W4E3H3" ], "product": "Protein-export membrane protein SecG" } }, "id": "NKOCPLAKPK_2127", "locus": "GCA003260355_02123" }, { "type": "cds", "sequence": "contig_3", "start": 105329, "stop": 106306, "strand": "+", "frame": 2, "gene": null, "product": "Solute-binding protein family 5 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7W4EC86", "UniRef:UniRef90_A0A660MPV2" ], "nt": "ATGCGATACCGTAGTTATGAGTCGAGTTCTCCTAAGAGAATGTTGGCTTATTTTTTGGGAGTTATGGCTGGGGTTCGAGTAGCATTTAGCCTGATCCTAGGATATCAATTTCTTGTCGAAAATGGAGAAAAGACCAATCAAAAGGGCGGAACCTTTGTCGAGGCATTCTTTGATCAGATTTCTTATCTTCCCTATCTAAGGAATGATGGGCAGAGTCTTTTTTATCAATCTTTGCTTTTTGATGCGTGTATTGATTATACAACTCTGAATGAACAAGGATTACAGAACGGTCAATGTAGGATTCTGACTCAAGATTATCAGACTTATTATGTTTCTGAAGTAGGAACTGGGAAGTCGTGGTCTGATGGCGAGCATCGAGGCTTAGACGATATTTTCTTTACCTATGACCGCATTATTCGTCAGAATGTCTGGAATATCAAGTCGCTTAATGCCTATCATGAGATTAGTGTTGAAAAAGAAGATGGGAAGATTAAGGTAAAATTCCCTACTAGCACGACGGACAATAATTACTTTTTTACGTTCTCTATCCTTCCAAGGCATATTTTGGAGCATGCTCAGATGAATGACTATACTTCAGCCTTTGCTGCTGGTCCAATTACTAGTGCCTGCGGGAAAATTGTCCCTAAGAGTAGTGATGGATTGAGCTTGGTTTTCAATCTCATGCAATGTCAGGATACCAACTTTGCTTACTATCAAATTAAAAAGTATCCAAGCTTTGAGGATCTTTCTAGACTTCTTCTGGAAAACGGGAAAACTATCGTTGATGCCTATACTCACCAAGTCCAGCTTCCTAACTATAAAACTCTTAAGGTTATAAAAACAGACCTTTTGACACTCTTCTTCAATACTAAGTCTCCGAAGATGAAGGTAAGGCTAAGAAGAGCATTAGCTGGATTGATCAATAGTAAGTTTTATGTAGGAGATGAGTATGGAAAATTCCTTTGGATGTATAAGTGA", "aa": "MRYRSYESSSPKRMLAYFLGVMAGVRVAFSLILGYQFLVENGEKTNQKGGTFVEAFFDQISYLPYLRNDGQSLFYQSLLFDACIDYTTLNEQGLQNGQCRILTQDYQTYYVSEVGTGKSWSDGEHRGLDDIFFTYDRIIRQNVWNIKSLNAYHEISVEKEDGKIKVKFPTSTTDNNYFFTFSILPRHILEHAQMNDYTSAFAAGPITSACGKIVPKSSDGLSLVFNLMQCQDTNFAYYQIKKYPSFEDLSRLLLENGKTIVDAYTHQVQLPNYKTLKVIKTDLLTLFFNTKSPKMKVRLRRALAGLINSKFYVGDEYGKFLWMYK", "aa_hexdigest": "4954b60b96e9953fffe0a982b5f8cf19", "start_type": "ATG", "rbs_motif": "TTTA", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 105329, "stop": 106609, "observations": { "mutation": [ 106304, 106559 ], "stop": [ 106304, 106559 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A660MPV2", "query_cov": 1.0, "subject_cov": 0.4616477272727273, "identity": 0.985, "score": 655.0, "evalue": 5.27e-232, "gene_start": 1, "gene_end": 325, "reference_start": 1, "reference_end": 325, "reference_sequence": "MRYRSYESSSPKRMLAYFLGVMAGVRAAFSLILGYQFLVENGEKTNQKGGTFVEAFFDQISYLPYLRNDGQSLFYQSLLFDACIDYTTLNEQGLQNAQCKIQTQDYQTYYVSEVGTGKSWSDGEHRGLDDIFFTYDRIIRQNVWNIKSLNAYHEISVEKEDGKIKVKFPTSTTDNNYFFTFSILPRHILEHAQMDDYTSAFAAGPITSACGKIVPKSSDGLSLVFNLMQCQDTNFAYYQIKKYPSFEDLSRLLLENGKTIVDAYTHQVQLPNYKTLKVIKTDLLTLFFNTKSPKMKVRLRRALAGLINSKFYVGDEYGKFLWMYKGGLLKKFYSDGSNIKEFINRAALSEKEGVQQQDLQDSGVLELKKSISINGVERKFVFYTPKSDATFNLEIKFSNQFETIRIKDAKGHEFSPKNYKKTDKKVIYPLENGKNLNPGLNQYTIVGSIKGKTYTIANIDLYVLATSTQEKDDTSDWKVKVLYYNSLESNFAVKQLRTIFEKAGILENFLFEQVSSPEQFEAKIVLGEYDILLNTINMGMKKDVLKVLMTDDVMANPSKYTNPNLSNYFKQYTKNPEDSQIASEINAIYAQDMPLVILGHPYNFVNIEKSLISSDFGSGITLYEYNWRKYLYSQVALIKNTEINFEKIKDIRGFLQYLQSKSGVSFPLPEMKTNVETGEKEHESSTPSQVVDGDPFAGLLKPAE" }, "paralog": false, "identity": 0.9812206572769953, "score": 842.0, "evalue": 7.35e-308, "description": "Internal stop codon at 106304, 106559. Nonsense mutation around 106304, 106559.", "psc": { "uniref90_id": "UniRef90_A0A660MPV2", "product": "Solute-binding protein family 5 domain-containing protein", "uniref50_id": "UniRef50_A0A7W4EC86", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A660MPV2", "UniRef:UniRef50_A0A7W4EC86" ] }, "pscc": { "uniref50_id": "UniRef50_A0A7W4EC86", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7W4EC86" ], "product": "Solute-binding protein family 5 domain-containing protein" } }, "id": "NKOCPLAKPK_2128", "locus": "GCA003260355_02124" }, { "type": "cds", "sequence": "contig_3", "start": 106388, "stop": 106561, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCAGCAGCAGGATCTCCAAGATAGTGGAGTTCTGGAGCTAAAAAAGTCAATCAGTATCAATGGGGTTGAAAGAAAGTTTGTTTTCTATACACCAAAGTCTGATGCTACTTTTAACCTGGAAATCAAGTTCTCCAACCAATTTGAAACCATAAAGATCAAAGATGCAAAATGA", "aa": "MQQQDLQDSGVLELKKSISINGVERKFVFYTPKSDATFNLEIKFSNQFETIKIKDAK", "aa_hexdigest": "3d2b0c15443eb811afa487993e8f21c2", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6597.4601999999995, "isoelectric_point": 8.008343315124513 }, "id": "NKOCPLAKPK_2129", "locus": "GCA003260355_02125" }, { "type": "cds", "sequence": "contig_3", "start": 106604, "stop": 107125, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGTTTATCCTCTAGAAAATGGAAAGAATCTGAATCCAGGACTCAATCAGTATACTATTGTTGGTAGCATAAAAGGGAAGACCTATACGATTGCCAATATTGATCTCTATGTTCTTGCTACTTCGACACAAGAGAAGGACGACACGAGCGATTGGAAGGTAAAGGTTCTTTACTATAATAGTTTGGAGTCTAACTTTGCGGTAAAACAGTTGAGAACGATCTTTGAAAAGGCTGGTATTTTGGAGAACTTCCTCTTTGAACAGGTGAGCTCTCCTGAACAGTTTGAGGCAAAAATCGTCCTTGGAGAATATGATATTCTCCTCAACACTATTAATATGGGAATGAAAAAAGACCTCCTTAAAGTCCTCATGACTGATGATGTTATGGCAAACCCTTCTAAATACACCAATCCAAATCTTAGTAACTACTTTAAGCAATATACAAAAAATCCAGAAGACAGCCAAATTGCAAGTGAAATCAATGCAATTTATGCTCAGGATATGCCACTTATCATCCTCTGA", "aa": "MVYPLENGKNLNPGLNQYTIVGSIKGKTYTIANIDLYVLATSTQEKDDTSDWKVKVLYYNSLESNFAVKQLRTIFEKAGILENFLFEQVSSPEQFEAKIVLGEYDILLNTINMGMKKDLLKVLMTDDVMANPSKYTNPNLSNYFKQYTKNPEDSQIASEINAIYAQDMPLIIL", "aa_hexdigest": "5dbe38f25e313b292f591d0f294d261c", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19684.2932, "isoelectric_point": 4.794505500793457 }, "id": "NKOCPLAKPK_2130", "locus": "GCA003260355_02126" }, { "type": "cds", "sequence": "contig_3", "start": 107624, "stop": 108163, "strand": "+", "frame": 2, "gene": null, "product": "Site-2 protease family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A9E5GRJ5", "UniRef:UniRef90_A0A660MQL1" ], "nt": "TTGATTTCTATTGGTATTCATGAGTTTGCTCATGCTTATAGTTCTTATAAGCTCGGTGATCCTACTCCAAAAATCCAAGGAAGACTGACTCCAAACCCTTTGAAACATATTGATCCGATCGGATTTTTGATGATTTTTTTGATTCATTTTGGGCGAGGGAAACCGGTACAGATTGATCCTAGTTATTACAAGAAGCCCTATAGAGATGAGCTTATTACAGCTCTGGCAGGACCTGCGAGCAATATCGTGCTTGGAATCCTAGGAATCCTCATCCTCATGATTTACGGAAAACTCCTCGGGACTTCAGCGATTGCACTTTTCAATGCAAATGATCTCGTTATTCAGTTTTGGATGCATTTTTCTATGATCAATTTTGGGCTTGCTGCCTTCAATCTCATCCCACTTCCTCCACTTGATGGATACAGACTGGTAAAAATCATTTCGCACAAGGCTGGCGCTTGGATGGAAAGAAATATGAGATATATTGCGATTGGTTTCCTGGTTTTAGTGGTATGGGGACCGCTTTCAGATGCTCTTTGA", "aa": "MISIGIHEFAHAYSSYKLGDPTPKIQGRLTPNPLKHIDPIGFLMIFLIHFGRGKPVQIDPSYYKKPYRDELITALAGPASNIVLGILGILILMIYGKLLGTSAIALFNANDLVIQFWMHFSMINFGLAAFNLIPLPPLDGYRLVKIISHKAGAWMERNMRYIAIGFLVLVVWGPLSDAL", "aa_hexdigest": "134beff9d95e9c09984ba3896eeba53d", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A660MQL1", "query_cov": 1.0, "subject_cov": 0.8136363636363636, "identity": 0.9940000000000001, "score": 361.0, "evalue": 1.08e-125, "valid": true, "product": "Site-2 protease family protein", "uniref50_id": "UniRef50_A0A9E5GRJ5" }, "pscc": { "uniref50_id": "UniRef50_A0A9E5GRJ5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A9E5GRJ5" ], "product": "Peptidase M50 domain-containing protein" }, "genes": [], "id": "NKOCPLAKPK_2131", "locus": "GCA003260355_02127" }, { "type": "cds", "sequence": "contig_3", "start": 108289, "stop": 108825, "strand": "+", "frame": 1, "gene": "prfA", "product": "peptide chain release factor 1", "db_xrefs": [ "GO:0006415", "GO:0016149", "KEGG:K02835", "SO:0001217", "UniRef:UniRef50_A0A288DCI2", "UniRef:UniRef90_A0A660MQ18" ], "nt": "ATGCTTGAGAAGTTACAAACCATCATCAATAAATATGAAACCCTCAGAGAACAGTCGCTCCAGCCTGAGATTTTTGAAGATATGGATAAAGCCAAGAAAATCAATAAAGAGCTTGCAGGCTTAGAAAAAACTTATGATCTTGCCTGTGCTTACAAAAAAGCACTTGAAACTGAGCAGGGAGCATTAGAAATTCTTAATAATGAAAATGATCCTGATTTACTTGATATGGCAAAAGAGGAACTTGAAGAAGCAAGAGCTGCTATTGAAAAACTTGATTATGACCTCAAAATCGCCCTCTTGCCGAAAGATCCGAACGATGAGAAGAATATTTATCTAGAGATCAGACCTGCTGCAGGTGGAGATGAAGCTGGACTTTTCGCAGCAGAACTCTTGAAAATGTATCTCACGTTCGCGCAGAAAAAAGGACGAAAACCTGAAATCGTGGAGGAACAGCTTTCTGATATCGGTGGAGTAAAATTTGTGATGGTAAAAGTGGCTGGAGATTCGGTCTATTCTCTGATGAAGTTTGAGAGTTGA", "aa": "MLEKLQTIINKYETLREQSLQPEIFEDMDKAKKINKELAGLEKTYDLACAYKKALETEQGALEILNNENDPDLLDMAKEELEEARAAIEKLDYDLKIALLPKDPNDEKNIYLEIRPAAGGDEAGLFAAELLKMYLTFAQKKGRKPEIVEEQLSDIGGVKFVMVKVAGDSVYSLMKFES", "aa_hexdigest": "539f199dcf28a3cfef9a18015f1d7ef2", "start_type": "ATG", "rbs_motif": "TTTTT", "genes": [ "prfA" ], "truncated": "3-prime", "pseudogene": { "start": 108289, "stop": 109128, "observations": { "mutation": [ 108823 ], "stop": [ 108823 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A660MQ18", "query_cov": 1.0, "subject_cov": 0.4958217270194986, "identity": 0.983, "score": 331.0, "evalue": 6.36e-112, "gene_start": 1, "gene_end": 178, "reference_start": 1, "reference_end": 178, "reference_sequence": "MLDKLQTIINKYETLREQSLQPEIFEDMEKAKKINKELAGLEKTYDLACAYKKALETEQGALEILNNENDPDLLDMAKEELEEARTAIEKLDYDLKIALLPKDPNDEKNIYLEIRPAAGGDEAGLFAAELLKMYLTFAQKKGRKPEIVEEQLSDIGGVKFVMVKVAGDSVYSLMKFESGVHRVQRIPDTESNGRVHTSTVTVAIMPEAEDVDIQIDPKDIVMDTYAASSSGGQNANKNQTGVRLHHLPSGLIVNIGDSKSQMQNKEKAFAVLKSRLYQIELEKKQAEEKSLRGNQIGTGNRSEKIRTYNFPQDRITDHRIHESWSNLPSIMMGNIDDIIGKLVLENQTMLLNQEMQGGS" }, "paralog": false, "identity": 0.985663082437276, "score": 536.0, "evalue": 7.26e-195, "description": "Internal stop codon at 108823. Nonsense mutation around 108823.", "psc": { "uniref90_id": "UniRef90_A0A660MQ18", "gene": "prfA", "product": "peptide chain release factor 1", "uniref50_id": "UniRef50_A0A288DCI2", "kegg_orthology_id": "K02835", "go_ids": [ "GO:0006415", "GO:0016149" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A660MQ18", "UniRef:UniRef50_A0A288DCI2", "KEGG:K02835", "GO:0006415", "GO:0016149" ] }, "pscc": { "uniref50_id": "UniRef50_A0A288DCI2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A288DCI2" ], "product": "Peptide chain release factor 1" } }, "id": "NKOCPLAKPK_2132", "locus": "GCA003260355_02128" }, { "type": "cds", "sequence": "contig_3", "start": 108835, "stop": 109368, "strand": "+", "frame": 1, "gene": null, "product": "Peptide chain release factor 1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A380NWX4" ], "nt": "GTGCAGAGAATCCCTGACACCGAATCCAATGGAAGAGTGCATACTTCTACGGTGACCGTTGCGATTATGCCGGAGGCGGAAGATGTGGACATTCAGATTGATCCGAAAGATATCGTAATGGATACTTATGCTGCTTCTTCTTCTGGTGGTCAAAATGCCAATAAAAACCAGACCGGAGTCAGACTTCACCATCTTCCAAGTGGACTCATTGTAAATATTGGAGATAGTAAATCTCAAATGCAGAATAAAGAAAAAGCCTTTGCCGTTTTGAAGTCTAGACTCTACCAGATTGAGCTTGAAAAGAAGCAAGCTGAAGAAAAAAGTTTAAGAGGAAACCAGATTGGAACGGGAGATCGTTCAGAAAAGATCAGAACCTATAATTTTCCTCAGGATAGGATTACTGATCATCGTATCCATGAGTCATGGTCTAACCTTCCAAGTATTATGATGGGAAACATTGATGATATTATCGGCAAACTCGTGCTAGAAAATCAAACCATGCTGCTTAATCAAGAGATGCAAGGGGGGAGTTAG", "aa": "MQRIPDTESNGRVHTSTVTVAIMPEAEDVDIQIDPKDIVMDTYAASSSGGQNANKNQTGVRLHHLPSGLIVNIGDSKSQMQNKEKAFAVLKSRLYQIELEKKQAEEKSLRGNQIGTGDRSEKIRTYNFPQDRITDHRIHESWSNLPSIMMGNIDDIIGKLVLENQTMLLNQEMQGGS", "aa_hexdigest": "82bf86ac4fd55629ed40334eb115537d", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A380NWX4", "query_cov": 0.9887005649717514, "subject_cov": 1.0, "identity": 0.52, "score": 180.0, "evalue": 7.49e-55, "valid": false, "product": "Peptide chain release factor 1", "uniref50_id": "UniRef50_A0A380NWX4" }, "pscc": { "uniref50_id": "UniRef50_A0A380NWX4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A380NWX4" ], "product": "Peptide chain release factor 1" }, "genes": [], "id": "NKOCPLAKPK_2133", "locus": "GCA003260355_02129" }, { "type": "cds", "sequence": "contig_3", "start": 109555, "stop": 109728, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAGTGACGAGGACTTATGTAAAAGTAGCTCAGCAAAATTCAGGTGATGGTTCTGCTCAAAATCCTCAGTGGTATCCAATTGGAGGAGAATTAAAAAGATCCGGTAATACAGTTGTTTGCTCTGACTCTAAGTTCAAAGTTACTGGTTGGATGCGGTATCAGAAAGAGTAA", "aa": "MEVTRTYVKVAQQNSGDGSAQNPQWYPIGGELKRSGNTVVCSDSKFKVTGWMRYQKE", "aa_hexdigest": "391ed230de47f99f56dd3eb9b007bd13", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6442.149900000002, "isoelectric_point": 9.300741004943848 }, "id": "NKOCPLAKPK_2134", "locus": "GCA003260355_02130" }, { "type": "cds", "sequence": "contig_3", "start": 110270, "stop": 110416, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGAGAATCTCCCGTTTGGTATATGGAATTGAAAACAATAGAAGGAATCTACTCTATAGCTGGAGTGGGACTTGATGGAAGTTCCCTCTGCAAGTATATGGAGAAGACGGAGTATTTTATTGCTGTGGTATTGATCAAAAAGTAA", "aa": "MRESPVWYMELKTIEGIYSIAGVGLDGSSLCKYMEKTEYFIAVVLIKK", "aa_hexdigest": "27c1c2b3ccedbb29ec4d0fbc98002607", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5491.4628999999995, "isoelectric_point": 6.113681602478027 }, "id": "NKOCPLAKPK_2135", "locus": "GCA003260355_02131" } ], "sequences": [ { "id": "contig_1", "description": "[organism=Absconditicoccaceae [g1] bacterium hmt-345] [strain=HOT-345] [gcode=11] [topology=linear]", "nt": "CTCGGATTAACAGGAGAAGCGAAGGTTACGATTACTTTGAATGATGTGGATGAGATTCCGCCAGTGATTAGTCCTCTTACTGGAGTGAGCTATCAACGTTCTACGCCTATTGTGCCTATTCCATTGGTAGCAACTGATAATAGTGGATCGGTAACGCTTCAGGTAAACGGATTACCAGCAGGATTGACCTTTGATATGACGGGTTCTCAAATTATTGGAACGACTCCAGCTACTCCAGGAAGCTATCCTATCCAAGTGGTTGCGATTGATCAGGCAGGAAACCAGACTACCGTTCAGTTTACACTTACTGTGATAGCTCCTCCAGCTCCTAGTAGTAGTTCAAGTTCCTCTTACCGTTCTGGTGGAGCAGGAACAAGCTTACCAAAAGATAATTGTCCTAACGGTGATTTCTCATCAAGCTATTATGATGGTAGCTGTGGAAATGAGTGAAAGCCTGTAGAAACAAAAGAGGTAGAAAAGAGTCTGTTTAATCCAACAATTAAGACGACGTGTTTTAGTCCGCTTGATCAGAAAACGATTGATCAGGGAAATGCTATGACAGAACTCTTGAGACAAGCTCATCAGATGCTCTATAGTTATCAGCTTACAAGATGGCAAGGCACCAAAGACTTTGCTCCAGAGAGAAGCTTAACCAGACAAGAAGCAGCAAGATTTATGACTGAATTTGCTACAAATGTGCTTTGTAGGAAACCAAGTAGAAACTATGCACATCAGTTCATAGACTTAGGAGATGCTGATCCAACGCTTCTACCTTATATCTATAAATCGTATGATTATCTTATCTTTAATGGGGATGGGAATCCAAACGGAGATAAAGCACAAACGACGTTTAGACCTTATGATCTGATAACGGTAGATGAACTCTCTGCACTTCTCACCAGACTCGTGAAGAATCAAACCATGGAAGAGCCAGCAGAAGATCGAGCAAGAAATTATAGGAATTATATTAGTTCTATCGCCTCAAACTCAGCTTTAAAGAATGATATTAGATGAACGGTTGCTGAGGTATTGTATGATTTGTATAGAAATAATAGCTATGACCTCAAAGAAGTAGGCTATGTGATTAAAGACTAAATGAAATGTATAAAAGAAAAGGGAAATGAAGTCAGATTTTTTTCCCTTTTTTTGTAAAGGTAAAAGTCCCCAGTAAAAAACTAGGGACTTTTGGTTAATGGTTAATAATAATTGACTAGTAGATAGCGACCTTTTTCGTTTCTTGGTCGCCGATCCTTACAACGTAGATACCCTGTTGTAAAGATACTGCATTGTCGCCATTCATCTTCTGATGACGAATTTGTTGCCCGCTTATGGTATAGACTGAAACTTGTTCAGGTTTTACCAGTCTAATATGGAGTATGTTGCCATTGCTCCATATGCTCGGCTCTTCTGGATAGAGCTCATTGCCTACTGTAGACTGAATATCAATCTTGCAGCTGGCAGAAACTCCGCTTCCATCAAGTGCCTTGACAGTGATCGTAACTTCTCCTTTTTTGAGGATAGTTACATGACCATTCACAACCGTGGCAATCGTAGGATCACTAGATTTCCATACTATATCCTTAGAGGTAGCATTAGTCGGTAGAATATCAACACTAAGTTGATAGGAGGCGACTCCATTCAGCGTAACTAAAGTATCGCTAAGGATCAGCTTTTCAATTTTTTGGTTATGTCCAGGTTGATCTGTGGTGGCAGCTTGGTAAATAGTTACCGTTTGGCTCAGAGAACCTGAGGTAAACCTTACTTGTCCTACCCTTTCTCTCCCTGTGGTATTCTCCTCAATATTGAGAGTTACTACTTCGTCTCCACTTCCCGAGGTTGTCATAGAGGTTATCCAAGAATCAGCACTGATAATAGACCAATCCTGATCTGAGGATACTCGGATCTCCTCTCTTCCCGCTTTTCTGTCTCTTCTGATTGCTCTTCTACTCAGGCTGAGTGTTCGAGTAGAAGGAGTCGGGGGAGTCGGGGGAGTTGGAGGAGTTGGAGGAGTTGGAGGTGTCGGCGGTGTCGGAGTAGCTCCATCTTGAGTGATCGTTACCGTTCTGGTCAAAGAACCAGCAGTAAAGGTCAGCGTGACCGTTCTCGGAGCAATTCCAGTGTTCTGAGCAAGTGTAACATTTACAGTTCCATTGTTCGTTCCACTAGAAGTAGAGAGCGTAGCCCATGACTGATCAGAAGTGGCAGTCCATGCCAAGTTAGAAGTGACAGTCAGTGTTTTCGATTCTCCAGCAGCAGCTACATTAGTAATAGATGCAGGAGCTACTGAAAGCATCGGTGCAGTTCCCGGTTGTGTGATTTGAACTTCTTGATTGGGAAGTCCGGTCGCACTGAAGGTCAGTTTTGCTGTCCTCGGGGAAGTGCCGGTGTTCGCTGTGAATGTAACTGTCACTGTTCCGTTGTTTGCTCCGCTCGCAGGAGAGACCGTAGCCCATCCTTGATTGGAAGCAACAGACCATACTGCTATATTGGAAGCTACGGTTACCGTAGCTGTTCCTCCAGCAGCTGTGGGTGAGAGTGTTGTAGGACTCACTGACAGTGTCGGAGTAGCTCCATCTTGAGTGATCGTTACCGTTCTGGTCAAAGAACCAGCAGTAAAGGTCAGCGTGACCGTTCTCGGAGCAATTCCAGTGTTCTGAGCAAGTGTAACATTTACAGTTCCATTGTTCGTTCCACTAGAAGTAGAGAGCGTAGCCCATGACTGATCAGAAGTGGCAGTCCATGCCAAGTTAGAAGTGACAGTCAGTGTTTTCGATTCTCCAGCAGCAGCTACATTAGTAATAGATGCAGGAGCTACTGAAAGCATCGGTGCAGTTCCCGGTTGTGTGATTTGAACCTCTTGATTGGGAAGTCCGGTCGCACTGAAGGTCAGCTTTGCTGTCCTCGGGGAAGTGCCGGTGTTCGCTGTGAATGTAACTGTCACTGTTCCGTTGTTTGCTCCGCTCGCAGGAGAGACCGTAGCCCATCCTTGATTGGAAGCAACAGACCATACTGCTACATTGGAAGCTACGGTTACCGTAGCTGTTCCTCCAGCAGCTGTGGGTGAGAGTGTTGTAGGACTCACTGACAGTGTCGGAGTAGCTCCATCCTGCGTGATCGTTACCGTCCTCGTCAAAGAACCAGCAGTAAAGGTCAGCGTGACTGTTCTCGGAGAAGTTCCAGTGTTCTGAGCAAGTGTAACATTTACAGTTCCATTGTTCGTTCCACTAGAAGTAGAGAGTGTAGCCCATGACTGATCAGAAGTGGCAGTCCATGCCGCGTTAGAAGTGATAGTCAGTGTTTTCGATTCTCCAGCAGCAGCTACATTAGTAATAGATGCAGGAGCTACTGAAAGCGTCGGAGTCGGAGCAAGAGGAGTGATACTGAATGAGTATTGAGTTAATCCTCCTGGTGTGTCATCGGGCAAGTCATTCAATGTGCCATCGCTCATTTTGACAGTAAAACTGGTTTTCCCAGTTGCTTGTAAATCTGACAGAGAGACTGATGATTCGTCATTCAGACTAGATAATACAGATCCATTACTATACCATGTAATGCTAGGAAAACCGCCAGGGATTGTCACGACATTGTTCTTATTGGATTCCGTGATCACGATATTACTCGAGACGTCTGCTCCATTGAACTGAGGAACGCCATAGTAGTTTACATGGAGATTGATCCTGAATTCTTTTTCAGATGCTCCTGTACCTGTTTTGAACTTTATCTCAACTGTGCCGGCACTGGTGATTTGAGCCGTTGTGATGCTCGAACCTTCTACTGACTGAATATCAGGAATCCCACTAAAATCTATCGTTACGGTTGCTCCGTTTTTGATGCGGAGGTTGTTCCATTTAGCGATACCTCCAGCGATTATGCGTGTTGCATCAAACTCTGTAGCACTTACCGTTCCTCCTGTTGAAGTTACGGTCTTGGTGTAGTTCGTACCGGCAATGATTTGCTGGCTGATCAAAAGGATGATACCTATCATCACAGTGATCAATTTTTTTCTCTTTGTTTTCATACTATTATCCTTTCTTTTTTTATCATTTCTTGTATTTTTTTTTGATGAATTTCTTTTATTTTCTATTTGGAAGGATATGTATTTTCCACAGAATGTCAAGATGAAAATCGGTCGTCTAAAAGATCAGCTTTTGGATTAAAGATAAGAAGGCGGTCTTTATTTAGTTTTCTAGGGAAAATATTGTAAAAAGCTGAATAATGGTCAGGATTTTTCTGTTGTTTCAATGTTTACAGCTTGCTTTTTCTGGTTTTGGAGGATGTGAGCGAGAGGAGTCGCATTTGTGGTAAAAAAAGCTTTCTCCGTCAGGATTCTGATTTTTTAGCTTTTTTAGCAGTTTTTGTTCGTGGAGGATATGGAAAGAAGATGAAAAGTCCAAAAAAATTTGCAAACTTTGCTATTTTAGCTATACTTAGGGTGAAATCTTTGAGATGATTTTTACTTTCTATGCCAAAGATGATGAACACAATCTCTAAAAAAACGCTTTTAGCTGCCTTACTCGTAGTCTCTGGAGGGATCTGAGCGGTGACACTCGCTCAGGTCGATGTAAAGTCCAATCTAACCAATGCTGTGCAGAATATTCAGAAGGTCTATTTTACTCCTAATGGGTTGCCTAGTGATGATGAAATCTTGGTAGAAGCGTATGATGGACTTGTGAAGATTAGTGGAAGAGTGCTGCTCCAAAAAGACGGTGATGAAAACCTCCTTACTCCAGAAGCAGGGAAGACTTTTATTATTCAGGGAAAGAAGAATATAGTGTATGGTAAAGATGCTTTTTTGCTTGGTGGTAAGCTGAATAAAGCCTCGACTTGAAGTGTCGAAGCGTCTATTGTCTGAGGATATGATGGAGAAGTGCAGACGGGAGTTCAGTATGGAACGCTTGTGGGATGATCGTATAATAAATTGGGAAGTGGGGTGAGTTCTGCTGTTATTGGAGGATCGGAAAATAAAGTAAAAGGCTCTCACTCTTTTATCGGGTGAGGAATGCTTACTGAAATCAATGGAGATGGAAGCTTCGGTGCGGGAAAGAATGTGAAGGCTGCCAGTAATGCTACAGATAGAGTTTTTCTCTGGAGTGATAATTATTCGTCTTGGTATCGTGTTGATGGGAACGAAATGCGTCTTAGACCAGTAAAAGAGGAGACCTTCCTTATTAGAAGTCTGAATGGGATCGGTATTGGGACGAATGATCCTCAGCTCAAGCAAGGTGTAGATGTGAACGGACTTGTCCAGATCGGAGATCAAGTGCTTGCCTGTGGTGGTGATGCTCAAGGAGCTATGAGGTATAGGAATGGATGCTTCTGTAACTGTAATGGTGCTACCTGGGTAGCGATGAACCCTTCTGCTAAATGTCTCAATGTGTGTAGGCAATAAATGTGGCTTCGTAAAGTAGCTTTATGAGTATAGCGGTACTGCTTTGTCTCGCTGCGTGATTTGTCGTAAAGATCGTTATAGTAAATTTTTATCTGAATAGGAAAGGATGCGCTCGTTTACCTCGTCTTGAAGTGAAGTGCAGTTTCTGCTAGAGCAGTCTCCTTTCCCTCCGACCAAAGAGGTGGAATCATTCTGATTTTCTGCCTCTTTTTTGCAAAAAATGAGACAAGGACCCTATGGAACGGCGAGGCTCTATAAAGCTTGTCTGCTCCAGCAGAGCTTTGCTTGCTCTGAGGCGCAGCTTCTCCAAGCTATGAAGGAGCACTACTTTGAGGGGCAGTATTTTTCTAGTTCCTATAGTGGAGCATGGCTCTTTTTTGCATTGGCAGAGAGGAAGCTTGGGGTCGATGTAGAGGTGATTAAGTCGAGGTCTGCTCTGCTTTTGGTGGCTATGGAGGCTGAGATGAAGCCTCTTTTCTGAAAGGCTGATTGGAAGAGTTTTTATCTGCTCTGGACTGCAAAGGAGGCGATTCTCAAAAGGTGGGATGCGAGGTCACTCGATTTAATGGATCAGATTTCGTTTTTTGCTGTAGAAAAAAAGCCTCTTCAGCTCTGAGGAGTGCATTTTGATCGAGAGCTGAAGTATGGCTTTGAGGGGCAAGCAGGGATGGTAAGGAGTGGAGAAAACTGATTGCTTGCGTGGAGTTTTGCTGATTAGAGCGAACTCATTGTGAATCGTGCGTTGGTCGATTTGCTTTCTTTGATGAGTCGGGTTTTGTTTGGAGAGGTGAGTCTTATGGTTCCTTGGACGAGGTTACCGTTTGAGATGACGAAGCTTGTGGTTCAGACTTTAGTCTCTTTTTGCATATCAAAGAGGTCTCCGTATCGCACAAAGCTTTCTTCTCCGATGTCAAAGACAGTTATATTGGTCTTTTTGAGTTCATATCTGGTGCCGAGCAGTTCGATAAATCCAGCTTGGAAAAACTGGTTTTTGAGGAGGTTGATATCAAAGGTCACGGCGTAGGTTCCTTCGTTATCTCCGCTAGCGAACTTTCCATCGCTGGTTTTTTTGGTAGAGATGAGTTCGTTTTTGAGCGATTCGGTATTGAGGTGGGAAGTGATTTTTTGTTTGATTTGGAAGCGGTAGAAGAAGAGTGCAGCTTCGTATCTGGTCAATGGGAGTTCAAAGCTGAGTGCATCTTCGTTATTCAGGATTCAGAGCTCTTTTGCTTTGAGGAAGTAGTCCTTCCATCGAGGGTTCCCGTTTTCGTTGAGTCTTTTTCCCTCGGAAAGTCTGATAAGCATTGTGATGAATTCAGATTTTTTTAGTATGGTTTGTGGGCTAAAGTAGCCATTATTTCCCTGCATAATGCCGAGCTTACATGCTTCTTCGATACTTGACTTGAGTGAGCTGTCTGCCTGATTGAGATCTCTGAATTTACATGGGAGTGCTCCTCCTGTGAGGGCAGAGATATTGAGTGCTTTTGCAAACTGGGTGAACATTTTGGCGGCCTGTTCCCTCGTTAGCTGTGCGAAGGGTTGGTAGGTCGAAATTTCCCTTGAGCTGGTCATTTTCTGATCATACATTCGAGTGATGGCTTCATTGACCTCGGGAGAATTGAGCACGCTGAGTGAGCTTGTCGTGCTTGAATTATTGCCGCTCATGAGTCCGAGGATATCATTGTTTTGGGTTTGTGAGGTTTGTGTGCCGGTTTGGAGCTGAGTGCCTGTTTGGGTTGTAGTAAGAAAGGCTTGAGGGTTCGTATCGATCGTTGAAAGCTGATTTTTTGCTGCTTGATGAAGCTGCTGATTGAGAATAATTGCCTTGAATCTATAGATAAGTAGTGCGATCTCTCCTCTTGTGACAGGTCTCTGGAGCTGCATTACTTGAGATTCTTGGGTGAGGGAGATTCCTTTTGCTTTGATAAAGTAGAGTGTCCATCGAGGATCGAAGCTTTCGCTACTTGATTTGCCTTCTAGGATTCTAATGAGAACGGTTAGAGCTTCAGCCTTGGTAAGTGGAGTATGTGGGTGGAAAAGTCCTTTATTCCCTTTGAAGATGCTTACTTTACAGGTTGAGGTGATAAACGGAGCTAGCGTTGGATTGAAGAGTTCGCGGTCCTTGAAGTTGCATTCCTCAGTTTTTGTTTCCTGAGGGAAGCCTAAAATGCTGTAGGCCTGTCCGATGATTTTTGCAGCCTCTTCTCTAAGAAGCGGGTCTTCTGGTCTGTAGCCGCTTAGGGTATCATACTGCGTCAGTCCATTTTTGTGCATTCGGAATATCGCTTTTTCAAGTTCTGTTCCTCAAGTGAGTTCCATATCTAGAGGACTTGGTTTTTGGCTTGGCTGAGAAGGGGCCGTTCATGATTGAGAGGTAGAGCCTGATTGATTTGTTGCTGCTGGAGCTTGACTATTGGTATTGATCGTTGTTCCGCTAGAAGAAAAATTTTCACTAGAAAAAGTTTGATTTCCTGATTGGACGAGGATTTTTTTATTGATAGGATCGATGACGATTTTTTGATCGCTGGCGAGGGCTGGAAATGTGTTGAGCAAAGCCAACCCGAGAATGAGCATTCAGATTTTTGTTTTCATGATTGAAGATACGAAAGAGTAAAACTTCTCTGCTTAAAGGTATGAATACTGCCAAGATTTGCAAGAGAAAAATTGCTGGTTCTCCTCGTGCATTGCTCTTGATCTTTGTGTTATGCTTGGAAATAGCTTTCTATCTTGGTAATGATCATTTGCACGTCTTGGTCGAGGTTGCCAGAGCCTTGGATTTTGAAGCCAGCGGCGTTTTGATGTCCTCAGCCTCCAAAAAATCTTGCAAGTTCTGAGCAGTCTACTTCTCCTCTGCCTCTTAGCGATCCTTTGATGTAGATTCCGACTTTTTTGATGAGAGCTACGAGCTGGTTATTTCTGATATCTTGCATGATATACAGCCCGTAGTCTGCTTCGTCATGGTCGATTCCGTATTGCTCGAGCTCGTGGTCTTCATAGTAAGAATAAACGAGGCGGAGCGTTTTTCCATCAGGGAGTCAGATTTTGATTTTTTGCATTCTCTGCAGTAAATGCTGCATAAACTGCACAGAGCGGTAGGTTTTATTGCGGAATATCTCATCAATGATGAGCTTTTTTTTGGCTCCGTGCCTGAGAAGATCCGCAGCGATAGTGAACGACTTTACAGACTGTTCTCCCTCGTCATAGCGAAAGTTTCCGCTGTCTGTCGAAAGTCCCATGTAGAAATGGGTTGCAACCTCCTCATCCAGTAAGGATGGTCGTCGGCTACTTGTGAGTTCGTAGAGGATACTACAGGTGCTGATTGCCTCTACATCGCGATAAATAAGGGTATTGGCAGGTTCTTTTTCTGGTTGGTGATGGTCGATGATGATTTTTGGGTAAGGATCGAAGTAGTCTTCATGCCCAAAAGTAAAAAGTCAGATTCTCTGATATTGGTTAAAGTCTAGAAAAACAAGGAGATCATATTTACCATAATCAAACTCATATTGAACCTTTCAGTTTAGCTCGAGAAAATCGAAGACGCGACTTGGTTTGTGTGGCGTGAAATAGGAAACCGTTTTCCCTTGCTTTTCAAGGAGTTTTCCCAAGCCTAGAATTGCTCCGAGGGCGTCTCCATCAATGTGTTCGTGTCCAAAAAGGGCGATTTTTTGTGCGTTTTCTAGTTTGAGTTTGATGGCTTCTTGCATCGTTTCTGATAGTTAAAGTAAAGGGAAAGGCAAGAATATCGGTTCACGATCTAATTGCAAGAGAAATGCTGGTTTTTTTCAGATTTTTTGGTTTTTATGAAGGATTTCCATCCTTCTCGATATCATTTTCGTATCGCTTTAACGATAGGTATATTGTTTTTCCCGTTCCAAAGTGCTTTGAAGCTTAGTCGTCAAGCGATTCCTCGAGAACTCCGTATGATGAAGATGATTTTTTGATAAAGATTCCCGTGCTTTTTTGCTCGGAGAAAGAGATTTCTTATTTTCTGCTCTGCTGCTTTTGGGGTGCCAATTCGGGCTTGTTCGAGGATGCTCTTTTCTCTCTCTTGATGTCTGACTTTTAGATCTCTAAAGACAAGGATCGGGATTCCCTGCTCTCGAATGCTATAAATAAAGTCCAGATCTTCCGCAATCCGGGCGATCGTTTCATCGTAGAGGGTTTTTTGCATGAGTTCGGCTTTCCCAAAGATTCCATTTCCACTGAACATTTTGATCTCTCAGTATGCTTGTCCTGGCTTTAGAAAGTGGATCATAGGACGAGCTAGTCGATAATTATAGTCTGAGAAGCCTTGATTCTGGATTTTGTCCGTATTTCTCCGCATAAGCGTTGGTGTGATGATTACTTCTTTTTGATAGCGCTGAGTGTGGGTTTGATGTAGCTTGAGGGTGTGTTCAAGGTATTGTGGATCGATTTCATTATCATCATCAAAAAGCTGAATATAGCTGCCTTTTGCGTTTTTGATCCCGAAGTTTCTGACGTAGCTTGCCCCTCCTTCATGGTCTTTATTGAAATCGCTATTGAGATGTGAAAAGATTCTGATTTTTTGGTTTGTTTCTTCAGGCTTTCGATCTTGATAAATTTCTTGCTGAAAGTGGTCCCGTTCTGATGTAGTAAAAGACCTATCACAGACGATGAGAATTTCGAAGTCTTGAAATGTTTGATTTTTGAGGCAGGTGAGAAGGTTTTTTAGAGGGAGAGGGGTCGCTCTAGAAGGAATCACAACAGAAATATTTCGATTTTTGCTTGTTTTCTCCATCAAGAATCTTATTTTAACTAAAGTTTTGATTTACTTTTATCTAAATGTAAATGTTTTACAAGAAGAACTATTGGCTTGTCTTTGGTGGTATTTCCTTGCTTTTGAGTGGTTTTGTTTTTTCTCGGAATTGGGGGAGAGAAATGATGACTACGATTCAGACTTCTTCTGGAGCTTTTTCTTCAATAGCGGATTCAGGTTTTGTTTCTTCTGGAGAAGTTCAGGAAACGACGGATGAAGTTTTGGAAGTTGCTGTCAGTGGACTTAAGTCTACGCCTTTTTGGACGATGAAGGGGGTGGCTCATGATTTCTGAAATCTCTTGGGCTACAATCAGGCAGAGACTATTATGAGTCAGTATAACGCTTGGACCTGAGCTTTTGATCCAAAAGCTAGCTATGAACATCTTGTTTCGCTGAAATTTCCGCTGAAATATCCGTATTTTAAAAAATATGTGCCTGAAAATCTTTCAGAACTTTTGGGACTTTTGGAGCTGAACCTTAGCCCTGAAGAGCTCAAAAATCCGAATATTCTCGTGGTTGCACAAACGCAGGAAGGGGTTGTCTTGGCGTATTATCAAGAGGGAAAGCTTAGACTTCTAACCAGTGCTTCTCCTGGGACTCCAAAAACTAAGACGGTCCAGGGAAAGTTTTTTCTCAAGCATGCAAATGTTGCCTATCGCTCTAGGACTTATGGTTCTCCTATGCCTTATGCAATGCGTATTAAGTGACCTTATTTTTTGCACCAAGCGGAAGTAGATGGGACTTTTCTTTCTCATGGCTGTATAAGGATTCCTGGGCTTTATCAGAAGTGGCTCTATGAGCATCTTCCCAATGAATCGGTGCAGAAGTCTTCTCATATTCCTGCGCCTATGATTATTGTTCATGATCTGTATCATCCTACACTTTTTCGCTATGATTAGGAAGGAAGTCTGAATTTGTTTCAACGAATGGATTTGTCCTTGATTTTCTTTGTTAAAACGCTATTTTTACTTTAGCTTTTAGCTTATTTTGAGAGATAATGTCGATATTTTTCCAAGTTTTGAGGTGAAGCTTCTATGTGATGACCGTGATTATGGGGATTTTTTTGGTAAGAGGTAATATTATCTTTGGGGCTGAGCTCTTTAAAGTGCTAAAGGAGGTTTTGATGCCGGGATATTTGGTGTTTTGCGGGATCATGATTGGGTATTTGATCGCTGTGATTTGGCAGGGAAAATTGCCTACTTCGACTGAGGTAATAAACACGAGAGAAAATATTTTTAAGAAGTCCTTTTTGATAGGGGTTTCTTTGGGGGTAGTTTTAGCTGTTTGTTATGTCTTCTACTAAAATGCCAAAGAAAATCTGGTTTATTTCTCTTTTGTTTTGTGTGCTTTTTTTGGCGTGATGTGGGGGAAAGTATCCTTTGTCTCTTGAGGCTTTTGATCTTACATGGACTCCAAAGTTGAAAGTTAAAATGCAGTTGGCTGATGAGGAGCCTGGAGTGTTCTATGTTTATCAAGAGATTGCAGATTCCTTTGAAAACCTCGATACTTTGATTATCGCTCAGGAAACAGGTGTGCTCCTTTCTCTGCCTGGCTATGCCCAGAATGCGATTAATCAGCTGAAGACTGAAGGCTATCTTTTGTCAGGAGAGGTCGCTAAAAAGCTTACGTTTCGTAGTAATGGTCGCTCTTCTTCAGCTTTGCTTAAGAAATATCGCTTGTGGAATAATGAACGTTCGCTCGAAGTAGCTCAGGTTTTTATTCTTGAGGAAGATAGAATGCGTATTCTTTCCTATATGAGTGATGAACCTTCTCACATTAAAAACTTTATATCACAGCTTTCTTCTTTAACCTTTTAGTTCCTTTGATTGCTAATGACGAATCCTACTACACCGACAAATTCTGTTTCCTTTACGATTCCTGGTATTGATGGAGGGATGAATCCTCTGAATCCTGGTGCGTTTGCTCGTTCTGAGAGAGAAAAATTAGATCGTCTTGTTAACGCTCCAAGTGCTATTTCGGGTTGGCAGCTTTTGTGAAAGATTGGTTTAGGAGTGGTTATTGGAGCCTTGATGGCTTTTGTCTTGTATATTATCATGGGGGCTTTCTGACTTGCTGTCGGGGGGAATGCTTCTCAGACGACCTTAGGTGCAGGAACGGCTCAGCTTACCCATCCGCTTGCGTGGCTGATTTTGTTATTTCTGGGGTTTTTTATTACTTTCTTGGGAAATTTGATTTTAGTCGTAAGTTATAGTGTCTTTTTCTCTCAAAAGTATACAAAAATCTGAAAAACCATAGGACTGCTCTTGTTGACCAATAGTATTTTATTTGCAGGAATGATTGCTCTTTTCTTATTTCTTAAAGAGGTGCCAATGGCTGAAGTTGTGCTTTTTGCTTTGTATCTTTGTCTTTCACTTTTTCTCTCTTTTTCACAGATGGAGTTTGTGGTGAATCCGAATTATTCGGCTTCTTCTCTCATAGGGAATGCATTAGGGCTTTGTCTCTGTCTTGTGGTGATTGGAATGCTGTGGTCACAAGTTTATTCTAGTGTGGATAAGATGATGCCTTATAGATTGATTTGGTATTCTACTTTGATTTCTTTTCCGTTGATGATCTTGGGCCAGGGTTTGTGGGAGATTATTTATGCAAAGGTATATGAAAATGGAGCCAATCCTTTTTATCTTCCATCACAGGCAGAACTCGATACACAGACGATTTTAGAGGGACAGGAGGAAGAACAAACTCAGGAGAGCGTGAATGTGGAACTTTAATCTTTCGTAAGTGAGAAAGTCAGTTTTTATCTTTTTAATTTGTCGATGCATAAGATAGCGGTGTTTCGTTTAGCGTCCCTTGCAAAGTTGGTTTTGTGATTGGCGATTTTGATTATTGATGCGAATCTTATTTCGGTAAAGGAAGATCCTGCGATTGCTTTGACGCTTGGTCTGATCGGAGTATTCCTTTTTTTTTGGGGGGCGAGCTATTTTTTGTTTTATGGAGTACAGTGGCTTGTTTCTACGAAAATTACTAAGGAAAAAATGCAGAGCGATGCCTATAAATCAGCATTTCTATTTGGGTTATTTGCTCTGATTAATGTATTGCTTTTGCTTGGGAAGTTTTGGACGAAGTGGATAAGTTTGGTTTTGTTAGGGGTGTTTTTTGCAATGCAGTATGTTATTTTTATGGATCCAAAGAGAAAAACTGATGAATGAACTTCAGCATAAGATAGAACAAGCACTTCAGACTGTATATGATCCGGATTTTCCAGTTGTGGATATTTATACACTAGGTCTGATCTATGATATTACTATTGAAGAGCAGAAAATTAAGGTTACTATGTCGTTTACCACGCCGGCCTGTCCTATGGCGGATTATTTGATAGCGAGTGTTGAAGGTGCTGTTCGTTTGGTGGCTCCGGAGTATGAAATTGCTGTAGAGGTTACTTTTGATCCTATGTGGAATCCTCGTATGATTAGGGATCAGGATCTTCAGAGGATGTTTGAGTAGTTTTCTTGGAGCTGTAAGTAGTGCAGTACTTTTTTAATCGTTAAGGTAATTGTCTCCTTGGAGGAAGCTTGGGAGTTTTTTTGCTATGAATGTTTCCGTTCCTAACTGTTTTTCTCAAAAGGAGCCAATACTCATTATTTTTGCTTCTTCCTGAGGAATTGAGACTGTATTGGATTCGAGGCAGAGTTTTTGCGGTTGCTGGCTAGTGTTTGCGATGATAAGATAATTCTCAAGTCCATAGAAGAGGTTTCCGTTGTGAAGCGCTCAAGCGTCTCCTTCCCATTTGCGGATTTTGAGTGATGAATCGTTTTTATTAAAGGCAAGGATTCCAAATTTTCGTTCTCCTGCTCAATACATTGAGAGATCGTTGAAATTGAAGGGGTGGACTGTGGTATTTGGCTGTAATGCTTCTGCAACTGAAGAAGTAGCTTCTTGATCGGAAAAAAGTGGCACAATCACTGAAAATCATGGTCAGACTTCTGTATTATTTTCTGGTATTTTGCAGTCCTCTGCTATCCAAGGGAGTTCTCATTTGGTTTTTGCGAGTCCGGATATTTCTAGAGAGAAAGCTGGCTTTCAGAGGCATTCGTCTCGAGATTTTTGTGGATTTTCGCATGAAAAATTGTGCATTAATGCACTGTCTCCGGAGCTAATACTATAAGAAAAACCCACTCCACGACTTCCCACTATTGCTAATCAGAGTTCTGTTCCTGCTTTAGCGAGGTAATTTACTTTTTCGTAGTCTGAAATTTCTTTGCTATATTTGATGAAATTTTTCATTACTAGTCAAATGAGCACTCAAATGAGCGATGAAGCAAGGAGAATAAATAAGGCGAGGATTGTGATATTTCCTCTTGGTCTTTCATGACGCATGATTGCTTAATTTATATCATAAATGTTTGGAGTTTTCCCTTGGAGTCCAAGGAAAAGTTGTATTGGGAAGACGCTTTTATTCTCTCGTCTCTGTGGATTGTAGAAGGCACTATAGATTGATCCCAATATTCGAAAGCCTGGTTTCCCAATATCGATTTCAGAATCTTGTTGATTGTTTGGGAGATAGGGGATGACCTTGAATTTCATTTTGCTCTGGTTGATGTTCCCGTTTCAGAGCAGTGTGATTGCGGTATCTGGAGCATATTCTTTTGTCAATAGTAATTTACAGCCAGAAAAATCTGATTTTTCTGTCTGGTATGAGGAAGTATAAAGATTACTACTTTTTTCGCAGTCACCGACTGAGGCAATTTCTGTACCGGTTCGCATTCCTCAGGTGAGATAAAGCGTATCAGTAATCCCCTTTCCTTGTTTGAGGTCGATCCCCTTTGCTTGGTATTTTTCGTAGTCAATAGTAGCTTCTTCTGCGATATTGTGAAGAACTTGTGAAAGAATGAGGACTTCTTTTGCGAGCTCTTTATCCTGCTCTATTCTAAAGGCAATTTTGGTGATGGTGGTATAGGTGCGGAGGATCGCTCCTGAAAGTAGTGCAAAGATTACAATTACGATAATGATTTCCACTAGAGTAAAAGCTTTTTTCATACTATGAGAAGGATGTTGAGATAAAATAAAGTTTACAGTTCGGCTGGGAGTGAGTCTTTCATTCCAATGAAACTTTCAAGAAGGACTTCTCCAGTATTCCCGCCTCTTTTGTAGAGGACACGCGAGCTGATTTTTAGTATTTTATTTTTGTCAAGAGGCTGATCTCCTACGTAAATTGGGCTATATTCGATGATTCTTGCGAATCCTTTATAGGGTTCTTCGCTTGAAGAGAGCGATAGTGGGCTATAATAGGTTAGTTCTTGATTGTCTTCTCCAGTGAAAAAGTTGAGGTAAAAGGTCCTGAAATTCTCCATGAAGTTTTCAGATTTTTCTGCTGGTTCAAAGATGTAGTAATTGCCACTTCCTGAGAGTCCTACTTTAAAATTTGTAAATCCTTCATCCCCTAATGATATTTGTTTGAGGGTATCGTCTTCGAATTTTTCCTGGATATAATTCCGGGTATATCATTGTTCTATTTTGCTGTCTCTGATATTGTAAGCGATTTCTAAGCCTTCTTTGGCGAGAAAATTTGCCTTTACTCTCATATCGATGCTATCGAAGTAGCCTAGGCTATAGGTTAATAAGCGGAGCACGGCTAAAATACCTACTCCAAAAATAAAGACTACAATAAGAGTTTCAATTAGTGTGAAGGCTTTCAGATTTTTCATCTCTTGACAAAAAATAAATTTTTCACCTGTAGGTATAGGAATCACTGAGTAAAAGTCAACTATCTCTTTAAAATAAGTGAAAAATCTTTACATTTACTGTAGATTTTATTTTTTCACTATGGTATGAAAAATCCTGAATTTAAAGTTAAGATCTCGGATCTTTTGATGAAGCAGGCTACGGATACGTTCCGTTTTGAGGGGAAATTTTTGGAAGAAATTCCTCAGCTGACTTCTGATGGAATCTCTGGAGAGATAACGTTGCAATCACTCAATCATAATGCTCTCTTGGTTACTATTGATGCCTTACAATGTACTTTGTGTGAGAGGTGTGATCGCTGTACAAAGGCGTATCTTCGCCCTGTTTCACTATCTGGATATACTGCAAAGTATGTGGTCGATCCGTGCGTTGATGGAGAGGATCCAGAGGAAGATGTCTTGCCTGTTGATGTGAAAAATGGAGTTATTGATTTGCAGGAGCTTATTTATCATGCAATTCAGCTTCAGGAGCCTTTTGTGAAGTATTGTCCTGATTGTGAAAATGCTAGTCTGGAAGAAGAGGATTTTTCAGATTTATAGTCTGATTTTTGTCTTTGTAGTAGATTACTCCTTGAAGAATTTGGCTTACTAGTGAAGATTATCTTGCATTTCTGCAATTAAACACTACACTTGAGTCAGTATTTATCTTTTCTTTTGAGAACTCATGACGATTGATATCAAAACTCTTGCTGGGAAGTGGCAGCGCCAACGAAAAGATCAGAAAATTTATCATACCAAAGAGGATGGAGGGAAACCAAAGTTCTATATTTTGGATATGTTCCCGTATCCGAGTGGGGCTGGGCTTCATGTTGGGCATCCAAAAGGCTATGTCGCATCTGACACGATTGCGAGAAAAAAGATGCTTGAAGGCTATAATGTTCTTCATCCTATGGGATTTGATACTTTCGGACTAGGAACTGAGCAATATGCGATGGATCACAAGATGAAACCTCAGGTGGTCGCTAAACAAAATATTGAAACCTATATCAAACAGCTTGAAAGTATTGGCTTCTCTTATGATCGAGAAAGGAGTTTTAGTACTGCGGATCCCAAGTATTTTAAGTGGACGCAGGCGATTTTTCTCAAGTTTTACAATCATTATTATGATGAAGCTGAGGGGAAGGCAAAACTAATCTCTGAACTTGAAGAAAAACTAAAAAAAGCAGGAAAATCTGAATCAGAAATCAGGTTTATTTTGAATAATGAAAGGTTAGCATATCTTGACTATAAACCTATTAATCGATGTCCTCACTGTAAGACTGGACTTGCAAATGAGGATCTTGATGATGGAAAGTGTGAAAGATGCGGAAGCGAAGTCGAACAAAAACCTATGAGGCAGCGAGTTTTGAGGATTACGAAATATGCTCAGAGACTGCTTGATGGATTGGATGCACTTGACTGGGATGAATCAATGAAAGAGCTTGAGAGAAACCGAATCGGAAAATCCGAAGGTTCACAGTTCAAAATGCAGGTCGTTGCGAGCGAAGCGAAGCAATCCAGTGAAGAACTTGCTTTTGAAGTTTATACTACAAGGCTTGATACTGTGTTTGGGATGAGCTTTGTCGTGATGGCACCAGAGCATCCTTTGGTTGCACAAATCACGACTCCAGAACAGCTAGAGACTGTAAAATCCTATCAAGAACAGGCAAAACATAAAACCCAACTTGAAAGAACTGAACTTCAAAAAGAAAAAACCTGAGTCTTTACGGGAGCTTATGCTATCAATCCTTTCAATAATCAGGAAGTGCCGATTTATATCGGAGATTATGTGCTTGCCAACTATGGAACAGGAGTAGTGATGGCGGTGCCTGCACATGATGAGAGAGATTTTGACTTTGCGAAGAAGTATGGATTAGAGATAAAAACTTCAGTTTTATATAATGATAAGGACTCTTTAAAGAATAAATTTTTTACATATCCTCGTGCTATATGATCTTCAAAGGACGTTCATTATGATAAAAATTCTATTTTTAGATTACCTGTTTTTTCTCCAGAACAATCTTGGTATGAATATTGTCCATTTATAGAAATATTAAAACCATTAGAATTACTACTTGACAACTGATTTTCTAAAATTATAATTAATGCTGAAAAATGAATTCTTGTAAACTCTTGACAGTTCTCATGATTAACTTCTGATGAAGCAATTGAAAAAATGCAAGCTTGGCTCGCAGAAAGAGGAATTGGAGGAAAGAAAGTGAACTATAAAATTCAGGATCGGGTATTTTCTAGGCAGAGATATTGGGGAGAGCCGTTTCCTGTAGTGTTCTGTGAGGAGCATGGAGTCGTTCCTTTGCAAGAGTCAGATTTGCCTTTATGTTTGCCTGATGTAGAGCACTATGAACCGACAGGAACTGAAGAAGGACCGCTTGCGGAGGTGAAAGACTGGATTGAAACTACTTGTCCGATCTGCGGAAAACCAGCGAAGAGGGAATCTAATACGATGCCAGGATGGGCGGGCTCGAGCTGGTATTGGATTAGGTATATGGATCCGAATAATGATAATGAGCTTGTTTCATCAGAGAGGGAGCAGTATTGGCAGAACGTAGATACCTATGTTGGTGGAGCGGAGCATGTGACGAGGCATATCATTTATGCGAGATTTTGGCAGAAGTTTTTGTATGATCTCGGACTTGTGAGTAAAGATGAGCCTTTTCAAAAGTATCAAAAAGTCTGACTCATTATGGCGGAAGATGGTCGTAAAATGTCTAAGAGACGAGGAAATGTGGTAAACCCTGATGATGTGATTGCTGAATATGGAGCTGATGTCTTCAGGACTTATGAGATGTTTATGGGACCTTTTGATCAGTATATTTCCCGAAATACCAATGGAATAAAAGGAGTGAAGAAGTTCTTTGATAAGCTTATTGCTATTTCAGCTAAAGTCAGTGAGACCTATCAAGATGAGGCAAAAATTCTGACGCTTCTTCATCAGACGATCAAAAAGTTGACGGAAGAGATTGATGACTTTAAGTTCAATACGTCTATTGCTCAGCTGATGATCTTGGTGAATGCTTTATCTGAAGTGGAGCAGGTTTCTAAAGCTACCTTTGAGGCGATTGTGCTGTTGGTTGCGCCGTTTGCACCGCATCTTGCTGAGGAGTTTTGGCAGTTGCTTGGTAATCAGTTTTCAATCTTTACGACGGGAAAATGGCCAAAATATCAAGAGCAATATCTTGTAAATACCGAGATTACGCTTGCAGTGCAGTTTAATGGGAAGATGAGGGGAACTTTGCAAGTGGCGGCTGACTGTAGTCAGGATCAGGTTTTGGATCTTATTAAAAAAGATGAAAAGCTGGCAAGCTATCTCGTCTGAGCACCAAAGAAAGTGATCTTTGTCCCAGGGAAAATTATCAATATTATTGTGTAATGTTAAGAGGCATTGCTTTCCTGTCTTTTGGAGAAGAGGTTTGGGGGAGCTGATGTCTCTTTTGTTTTGTTTGCCTAGGACTATGTGAAGTGGTGGCTTCTGCTTTTATGTCTTGTGTTCTTTGGGTGAGATTCTCTTGAGGTTCTATGAAAAGTTTCGCTTCCTGTGTCATGAGTCAGATTTTTTGCTGGTGAGCAATAAAGGGATTGACTTGATAAAAATATTTCTCCTAGGTGAAAAAAATAAAAATCGACTGGAAAAAAATATTTTTCACCGTAGAGAAATTTTTAGATAGACTGGAAAAATATTTTTTGTCTCTAGGAGGAGTTTTTTTGTTGACTTTGATCGTTATTTTCCTAGACTAATGGTGGTGTGAATACGTGCTTTAGTTTGTATTCGTTGTTAGGATGAAATTCAGACTTGTAGAAAAGGCAAATCCGCTTGATAGAAGCAAGAAAAAGTGGTATGCTAATCCCGCGAATACGGGCAAGATGGGGCAAAGGGAAATGTCGAAGCTGCTGGAAGAAAAATCCTCCCTTACGATTGGTGATATTTCTAATGTGATAGAGAATTTGCTTGCTGAACTTCCAAGACAACTGATTGATGGGAAGAGTGTCAGCCTTTGAGCTTTTGGGACGTTTCGTTTGACGTTTTCGAGTGAGGGGACGGAGGAAAAGAAAGCTTTTAATGCGGCATCGATTGAGCCGAGAGTTGTTTTTACTCCTTCTGTAGAGTTTAAAGAGAGGCTTAAGCATATCAAATATACTCAAGAAACAGAATAGTTTGGCTGCGAGGTGCTGAGTAAGCTATTTTTCTGTGTGAGGAGGATTTATTTTAGAACTGAAAGAATTTTTGGACTATGATGATCAAAAAAGAAAAATCTGACTTTATTCTGGTGATTTCTTGATGATGAGCAAAAGGTATTTATGCCTGCGGAATCTTGAAAGCATTGGAGGAACTTGATTTTAAAAAGCAGATAAAGGCGGTGTATGGCGTCAGCGCTGGAGCTCTGACCGGGGCGTATTGGTTGAGTGGATGGCATGCGGAGGCAATTTTTGAAAGGTTTCTGGAGAGTGAGATCCTGGGGCTGAAGAATTTTGCGATTCCTCCCAAGCTTTCTCTTCTCAAAGGGAGTACCGTTGAGGCTTTCTTAAAAAAAGATTTGAAGTCAGATTTTTCTGGCTTGGAAGCTGAGTTTTTTGTCGGAGCTACGGATATTATGACAGCAAAGTTTATTCTTTTTAATGACGGGAAGTTAGTTCCTCCTGTATTGGGGAGTATGGCGGTTCCAGGGCTGTTTCCTCCTGTTGCTTTTGAGCGTCATTTGCTCGTGGATTGAGGTGTTTTGAATAATTTTCCGGTTGATTTCGCTAGGAAAGCGTATCCTGATGCAAAGATTATTTGAATTTTGCTTGGAAAGTTTAAACAGCATCAAAAAGTGACTTCTCTTTTAGATGTTTTGTCGCTTTCTTATTCGGTTGCTTTGCAAGGGCGCTTGAGTAAAAATTTGGATAAGGTGGATTATCTTCTTTATAAAGATCTTGAGGTAGGAATGGTTGAGAGTTCTGAAAAGAAACTGAGGGCTCTTTTTAAGCAGGGGTATGAAGATGGTCTGAAAGCTTTTAGTTCTCAAAAGTAATGATGCGTTGATTGAAGTTTCCTTTTTTGAGCGCTTTGACCCCAAAAGAGCGTTATGTCCTTGTTATTTCTGGTGGAGGAATGAGGGGTTTTTATGGCTTGGGGATTGTGAGGGCACTAGAGGAATTAGGATTTAAGGATAGAATAGATGCGCTGTATGGTGTGAGTGCCTGAGGATTGCTAGTAAGTTATCGAGCGGCGGGGTATTCTGCGGATAAGATTCTTTCACTGTTCCTTAAGTCTGAATTCCTTTCTCTGACGAAAAATCTGAATTTTCTGCCTAAGGTTTCTCTCCTTAAGAATACTTTACTCAAAAAGCAGATGGAAAAGGAGCTCCCTAAGAGCTTTGAAGAGCTTAAAATTCCAACTTTTATTGGTTGTACAGAAATCAAAAAGGGAGAAAGTCTGATCCTTTCAGAAGGAGACCTCGTTTCTGCACTTTTAGGGACGATTGCTTTGCCGTGAATTTTTCCACCTGTTGAGAGAGAGGGGCAGTTATTGATGGATGGAGGAGTGACGAATAATTTTCTCGTAGAGCCTGCAAAGAAAGCCTATCCTCGCCATAAAATTATCGGGATTTCTCTCAACAAATATCGCACTAATCCCAAGGTAAAAAATCTGCTCGATAGCTTGATGGTTTCTTTTGAAATTATGCTGAAAAAGGATATTCCGGAGCAGGGAAGTTTGAGTGATATTTTCTTTTGTAGGGATTTGGATACGCAGGTACTGGAGTTTACTACGACGAAACTTAAAAAGATATTTAAGCTCGGATATGAAGATGGTTTGGAGAAGCTTCGTGCTTTAGGTTAGTGTTTTCACCATATAGGTTCCCTCTAGTTTATATCCCTGCTTGCGATAGTATTCCCTCACTCCAACTCCAGAAATCACAGACAGCTTTGAAAAGCCCGATTTTTGAGCGATTTTTTCTGCGGTTTCAAGGAGCTGTTTCCCCAAGCCGCTATGCTGCACTTTCTGCCCGCTTTCCTCTTGTGTTTTCAAACTCTGTAAAGAACCATAAACATGAAGCTCTCTAATCAAAGCTGTTTCCAAACCTAGTCCTGAAATATCCAGTCTTTCCTCAAGTTTTGGTGAAAGAAGCCTCGTAAATCCATAGAGATAACCGAGTTCATCTTCATAACTGATGAAATATTCTTGTCCAACTGAGCTTTCATAAGCCCTCACAACCAAATTCAGAACTTCAGTTTTTTCTTTTTTATTCCTTACCTCTCTGCTTCTGGTATCAAGGCTCACGAAATGACGAAAACTTGAAAGCTCAGGTGCTTTCCCGAGAAGATAGGTTTGGAAAGAAGCTGGCTGTGCGTCTTTGCGAGTGGAGCGAAGTAATCCAGTTGGAGCATTTTTTGGGATTGCCACACTTCATTTCGTTCCGTTCGTAATGACTGAAAAAAACTCCTCTTCATCACCAAAAACTTGCAAATTCTCATAGAGTCTGTGATAAAAAATAGAACGGAAGTCTGGATCAGCTTTTTGATACTTTTTCAAAAGCTTTTCATGCATAAGCTGAGAAAGATTCGTAACATTAGATCCAGCACTAATTTCCGTAGCAGGAATATCACGAATCAGCCTCTTGATCCTCGTATAGGGCGGAATAATCTCACGAAAAGTCGTCTCAATAATCTCACTAATCTCCTCTGTCGTGATCGGCTCATACTTCCCCTGCTGATAGAGCTCATACAGTTCCGTTTGCGGGATCACCGAAGTTGGGTACAACTTAATTTCATCAGGCTTGAGGTAGGAATTGGTATACACATCACGAAAAGTCTGAATATCCTTTTCCATCGTAGAACCATAAAGCCCCGGCATAATGTGAATAGAAAACTTGAAAGCATATTGCCTGAGCTTGTGAAGTGCCTTTTCTACCTCTGCGAGATGATGTCCTCTTTTATTCAAGTCCAAAACTGCATCATCAGTAGACTGCACGCCCATTTCAATCCTCGTTACGCCCATCGCTCTCCGATCTCTACAGTTCTGATCAGTCACAAATTCTGGCCTGGTTTCTATGGTCAATCCGATAATTCTGTGCTGTGCGGTTTCGTTGATCTGTATTGCCTCTTCCAAAGAAGAAGAAAGCTGAATCTGATCTTGGTTTGTAATCTCAAAAGCATATTTCCATTCCGCAGTTCCTGTCAGATTTTTGATTGCTAATGCTCAAAAGGTATTGCAGGCATCATAGAGCTGCTTGATAAAGTCAACTTTGTAGTCTCTCGGATAGAAATCCCAAGTTCCCCCCAGCACAATCATCTCAATCTTATCGGTCTTATGCCCCGTCTGCTGGAGAGAGTAAAGCCTATTGTAGACCTGCTTGAGCGGATCAAACTGGTTGAGAAAAGCTCTCATTGCCCCAGGTTCAGACTTGATATAGCTCTTGGGCATCTCCGGATCATTGGGGCAGAAAATACATCTTGAAGGACAAGGAAAAGGCTTGGTCAAAACTTGAATCGGAACGATCCCTGAAAGCGAACGAATTGCCCTTTTACGCAGCAAAAGCTCAAAATCAGCATTCTTTTCTATTTTTCCTTCTTCCAAAAGCTGAAAATATGCTTGGAGAATCTGTGATTTACTCGGAAGGGTGCTTAAATTATGAGATTTAGCAAAAGCTCTCTGGGCATCTTTCAAAGATTCGGAAGTCAGATTTTTTTCTTGTAAGAGAGTGAGAACGAGAGCGGAAAGTGGCATGCGTAATCGGTAATAATAAACTTGAGTCTACTTATATCAAAGGCTCCTTTAAAAACAAGGATAAGTCATCGTCTTTGCTTTGAGATGGAATGGAATGTGAAGCTTTTTCTAAAGAAAAGCACTCCCCTGAAACAAGAGAGTGCTAAGGTTATACCAGCTGTAGAAAGAAGCAGCTATTGTTTACTGTCTCATGCCGGTCGCTAAAGCGATGGTCCCACTCGGTGAGGCGTTTTTTATGCTAACCCTAAGAAGTGAAACTTTCCTTCTTGTATTGCCGAGTTTGGTGATCGTTCTGTGGGGAGGAATGGTTTCTTTCCTAGAGCGTCCTTTCCCTCCCTCGTTATAGTATTCGGTAGTTTCAAAAGTGAGTGCTACATTAGCGGTATTGGTGATACTTAGGATGTAGAAATCACTACCAGAGATGCCATAGAAGAGATGATCGGTGATTCCCTCTTTAATGATGCTTGTCGTAGCGGGGGGATCGACGGTGCCTCCGCACATCCGAACAATTTTTTCAACATTGACGGGATGGAGTCTGTTTCCTTCTCGGGATTGTTGCAGGTCTATGCTCCTTGAGTCGTAGCACAGGAGTGCAAAGCAGCCTGCTCCTAGAAAACAGAGTAGGAGCTTGGTTATTTTATTTTTATTCATATTACAAAAGTATTGTTGTGTTGCTTTTGTGAAGGTTGCTCGCTTGCTCTAGAGATGCTTTCCCAGTAATCAATCTGATTTTTAGAGCTGTGAAGATGTATTTGGATTTTTCACCGTTGAAGGCTGAGCTAAACAAGAACACCCCTCTTGAAAACAAGAAGGGTGCTACATGCTGTCCAATTAAAAAAGAAAAAATCTGATTAATTAATTAAGTTTATTTGTATTTGTTCTCTCAGGGTTTACACCGTGAGCTACTGATACTCTTCCTTTTGCAGAAGGAGTAAGTATCTTGACACCTACAGAGAAAATCCTATTCTCTGTATCATAGACTTCAGTGAAAGGTTTTCCCACTGAGATTGCAAGCTCGCGTGAGCCGAGAGTCTTACCAGCTTTGTCATATTCAATGACTTGGATGGTAATCGGTGCGTCAGAAGTGTTCTCCACACTGAGGACATAAAGATCATCACCGTTGATCTTCGTGAATCGGTGGGAACTCTCCGAATTTGCATGGAATTGCCAGCTCTTGGACAGAAAACAGAGGACTTTCCTCTCTTTCTTGACCGGAGAAGTTGGGGCATCCGAGACAAGGTTTGAAACCTTAGGGTCATTCGCATACATTTCGCTAACACTAGCGAAGAGGGCAATCAATATTGCCAATACTGTGATTTTTTTCATCTTATTTGTTAAATGTGCCTTTTTTTTTGGACAGCGGGAGAGACTATAGTGATTTGGGGGAGTGAAATCAACCCTTTTTTTGAAAAATTGATAAGATGTTGTGTTTTGAGCCATTTGTCAGATGATAAATCGTTCCCAGAGACGTTTTAAGCCATTTGTCAGATGACAAATTGCCCGCAGAGACGTTTTAAGCCATTTGTCAGATGACAAATCGTTCCCAGAGACGTTTTGAGCCGTTTGTCAGATGACAAATTGCCTCCAGAGACGTTTTGAGTCGTTTGTCAGATGACAAATCGTTCCCAGAGACGTTTTGAGCCATTTGTCAGATGACAAATCTTCTTCATTATAGAAAAAACACAGCCGAGAGGGCTGTGTCTGGGAGTATTTCCTCATTGATGGCTATTTGGTAAGGAGGTAGCCCTCTATTTCGTTATCAAGCTGTATTTTGCCGTTTTCGAATTTTGTTCCATCAAGTCTAGTGATGATGAATTTTGTATTCTCTTGTTCAATAATGAAAGGTTCTCCGCTTCTCTTTGATTGGGCAAAGATTTCTTCGAGGTAAGGAGTCAGATTTATTTCTTTGATCTCTCATGAAATGCTATACATAAGTGTATTGTCTCTTATCAATGGGGAGGTCCAATGAATCTCATAAAGCTTGTCATATGTCTGAGTAGTTATTCCCTTTCCCTCAAAGTTTGTATTGTCTGCTCGAAGACGGAACTCAATATCCTTACTTTCTTCTGGCTCTCGGTAGTAGTCCTCAATTCAAAGATAGTCACGGATCTCGTAGCCATAAAATCGGCTACTGTCTTTATCTGAATATTGGTTATTGAAGAGGACCTTATTTCGTTTTTCGTAAGGCTGAGTATTTACAAAGTCGATAACGGTTTCCGTCAACTCTCCTGCTCCATCTCCAGTCAAGTCTTTGAAGCTATCTTTTTGCAAAGGAAGCTTGAGGTTTGCTTTGGCGAGGAGGGATTCAATACGGGATTTCTGGGAAGAGAAGGCGATGGTTGGTGCGTTTCGTGGGCTGTAGAGGGAAATTAGACTCAGTCCGAAGAGCAACGAGATGAAAAGTCTGAGCCTCATCTTTGGTAAGCAAAGTGCCAAGACTGAGAATCCTATGATAAAGAGGATAAACATCCCTACGAAATATCTATTTATAGTAATGCCGTAGTCTTGGATTCTGAGTTGGAGAGCTTTGATCATCATCAATGCAATCAAGACGAAACTGATGAAGAGGATTTGGCGGATCTTATCAAAGAACCTGGAATTTTCTGCGAATGTAAGGAAGTACGTCAGCATTCCTCGAGCAAAATATCCTGTTCCCAACCACACGATGATGCCTTTAGGTCGAACGCCAGTGATGAGAATTTTTATTCCATAAGCGGTAAATATTGTCAGATAGATCAGTGCGAGCGGAAGGAAAATATAGCTTCCAAAGATTTTCATCAAGCGGGTAGATTGGAGTTCAAATTCTGAATGGCTGCCTTTGGGGAGGTATTCTGTGACGGAGCTATAATAATTGATCATAAAGCTTCCTGCGAGTAGGAACATACTGAATGTTCCAAAATATTCATACCATTTTCGGTTGATATCTACGTCAAAGAGTTCGCTGATTGAGCCAAGAGCTCCAGAGAGTCCTCCCCAAACAATAAGTCCCGCCAAAAGTCAAAATCCGACGGAATGGAGCAAACTTGTCCGAGAAAACCAGATTTTATGTTCTTTTTGCCTGAAAATTCGAGCGATAAGTGTCAAAATTCCTAGTATCAACAAAGGGAATATTCAAAAATAGAGGAGGTTCTCCGCGTAACGTGCATTTTCACTGAGTCCTTGTAGGAGTCGATAGTATCCTGCAATGGTAGGAAGTGCGAAGAGCTGGGTTATTCGATTGATGATCCTGATAGTCTTTGTTTCCTCTTCAGCATGGAGGGTAAGAAGGGGTCAGATGAGACTCACCAAGAATCCTAGTATTCCAGCGACTATGAGCTTGATTTGTCGAGTCTCATTTTGATCAATGTGGATAAAGTAGGTGCTACATCCAGTCAAGAAAAGTAAAATAAGATGAGCAACGGGGAAGGTCTTGAGTCCGTTTATTCGGTTTCAGAAGAATTGTTTGATCTTCATCTGGTTTTGAAAAAGGATAAAAAAGTAGTTTATGTTTACGAAAGTTTTTTACTGAGTCAAGGGAAGTTTGCTGGCGGTGGTTGAGGATGCCCGTTGCTTGGTGATCAGGGAAAGGAATTGTTACTTTCATGCTGAAGCAAGGCGATTTTTAGATCAAGACCTCCAGTATAGCCGACGAGGGCTCCGGATTTTCCTATAACTCTATGGCAGGGAATGATGATTGCAATAGGATTTCTTCCTACTGCTCCTCCGATCGCTTGGGCCGACATCTTTTTGATACCTCTAATGGAGGCTACTTGCTGAGCAATGGCTCCGTAGCTGGTAGTTTTGCCGTAAGGAATGGTTTTGAGTAGGTTTCGGACGGTTTGGCTGAATTCGTTGCCAATCGGATTCAAAGGAAGTTCGTTTATCAATGGTTTTTCTCCTGCAAAGTAGCGATCTAGTCGAGATTTGGCTGCTAAGAGGACGGGATTTTGGGGTTGCTCTTGGATTGGTTCATGATAGTTGCTGAGATCTGCGGTTTGTGGGGTGATTCGTAGACCGATGAGGGACTTTTCTTTGGCGGCAAGCAGGACTGTGCCTAGCGGTGATTCGTAGTGGCTTATTGCTATCATTTGGTCGCGTTATTTAGATGAAAGAGGAAAGGTCTTTCTTAGTATGATTTTTGTCCAGAGAAAGTCAATGCTAGTTTTTTGTTTGCTTTGTGGTGAGATGAGAGAATATCTTCTTTTCGTCTCTATAAAATCGAGGAATCTGGTCTTCTTTAAGAAAAAAAATCTGATTTTTTTGACTTTTTGGTTTTTTTACGTTATAATGGAGGTATCTAGAAAAACTATGTAAATAGATGAAAATTTTATTCTCTGTCTCGAGACACGTATGGCTATTGCTGAAGAAAATGGAAATCTTTTGATGGAATGATCACTTTCTGCACAACTTCAAGAGGCTTCAGATCCTATTCCTGAGGCTGATTTAGTGGAACAATCGCAAGCTTCTGCTCCTGAGGCTCATGTTGTTCAACAATCACAGCAAATTGAATGAATTGGGGATATGGGAGAGGATGCTCCTTTGGAACTGCTTGATAATGCGGGTGATTTGGCTGGACTGCAAAGTCAAGATGCTGCTTATGTCGTAAAAAGATATAATACGCAATTTATTCTTGCTGTTTTGGGAATTATCGGGCTTTGAGCACTTTCACTTGCTAGTTATTTTTTCTATCAGTATTTGGATTTGAAGTCTCAGCCTACGGTGCCGGTAGATAAGATCGAACGTGTGCAAAAAGTCTCTGATACCTATGATCAGTATGGAACGATGTTTGGGCTTTTTGATAAAGCTAAGTATGCTACACTTTCTCTTGCAGAGCAGGGAGAGACTTGAGCAAAACAGCTTCTTCAAGATAATGGGCTTAATTTTGTGCAGAAGAAAGATATTTTGCAAACCAAGCTTTCTGCTTTAGGAGAGCAGATTATTGCTCTAAAAACACAGACAAAAGCTTTCCAAGAAAGCATGAATAAAGATGGATTCTTTCCTCAAGAATTAGTCGATATTTTGAAAGCAGATAATGCTGTGGGATCTATTCAGAGAAGTTTACTTTCTCTAGAAGTAGTGAAATTTTCGAGTGCGATGAAGGTGTTTTCACTTATGGATGTTTTTGTTTCTTCCTTGGCGGAGAATCTTTGATACACCAAATCAGATATTAAAGAAAAACTTGATACTCTGATGGATAGAGGAGAAAAGGATGTAGAATACTATCTTAATGTCTGCTATAATAATCCTTATGAGCCTACAAAATGTGATAAAATTAATGATTTCGATGAGTATTATAAGAAGATTCTTGGTGATAGTTCCTTTGATACCGGATTCTTCAAGATTCTCATGGAGTTTATTAATAATAGGTTAGAATTTAGTAATATGCCGAGCTTTTCTATCACCTTCAACAGCTTTAATGGTAAGAGTAATAGTATTGGCTTTACTATCGAAGTGAATACTCTTAAGGAAGATGAATATGCGCTTATTCAGCAAGGTATTAAGAGTCCTCATATCTTTATTATCTCTGAATTGATTAAGTTGCTCAAGCAAAGTACCTTTATTGTGGGGAAGGCAATTGATGCAAAAGATATTAAGGTTTCTCCAAAGCAGGTTACTATTGGAGGATCGAAATATATCGTGAATAACTCAGTAAAAACCTTTAATCTTCCAATTCAAAAGACGACTGAGAGGGAAATCTTTGACTTTATGGATAGTTATATTTCAAGAATTAAGGATTCTCAGACGGATACTGGAGCTCTTGCTTTTAGTGGTGGGTTTTGAGCTTTTGTTGCTCAGCCTTTGGGGACTGGAGAAGGACTTGCTTCGTGATCGTTAGCTTCTGGAGAGGAGGGAAGACTTCTCTCAGATTAACTTAACCTCGTATAAAAAATCAGAATTTATTCTTTATTACCTTCTGAACATGTTAGAAAACAATTCAACTCCTCAGGTTTGATTTTTGGACAAAGTGAAGTCTTTTGTCCAGGATTTTGTGGCTTGATTTATGCAGAAAGGGAAAAATGGCAGTAAGCTAGAAATCTCTTTTGGAATTAATAAACAGACCTATCTCGTTACGCTTGGAATCTTTGGGGTAATGTCTGTGGTCACGGTTATGGCTCTTTTCTTTATCTGGTCTAAAAACTCTGAGCTGACAGCAAGGACTTGGGATCTCAAAGAACTTAATAAATATGATTTGACGAGCTTTCAACAGTCAGAGGTAATGGCTGAAGATAAGGATTCTCTGCCTCAAACTATTGAGGATATTGTTGGTTTGTATTCTTCTTCTAAAGAAGAAAAGGAAAGAATTGAAGGAGAGCTGACTTTTAAAAAGAGTGTTTATACTGAATTTTTGAGAAATCTTCTTTTACCTTCGCTTAATATCTGGAAAGATCCGTATACGAAGGATATCGATATTTCAATCTTGGGAAAAAAATATTTGGATACCAATCCTTACCAAGATATTTCATTACTTTCTCAGTGGAGTAGTATTATTAGAGAGAGTGGGAAAGATGTGGGAGACAATGAAGTTGTAAATATGAAGATTGGAGAAATTAATGAGTTAGAAAACGGATTTTTTAATATCCCGATTAGTATTTCATTTAAATCTGACTCAAAGAGAGCCTTCCTTCTTTTGGTAGATAAGCTTTCTCTGACTTCTAATATCAATAATTTGGGATTGCTCAATGACTTTTCTTACTATCTTTTCAATGTCATTAGAGAGAAAAAGGCTGAAGAAATTAATCAATTGGTCTCAGAATATCAACTTACGAAACCTCAGGATCAGAATGAAAAATCTTTTTATAATGCGGTTATCTCTCGTTATTTGTATAACCGATTGCAGACTCCAGATTCTGAATCTAATATCTTGATTGATGAAGAGGTAGTGGATGAGACGATCAAACAAAGTATTGTTTGTGGAATGACTGAATCTTCAGATATTTGTTATTTCAGATTTAGGGATAAGTATAGGACAATTCCAGGACTTGCCTATGGAATCGGACTTGAAAGTCTTGTGAATAAGCCTCTCTCTCTTAAGAGATTCTTTGCGAGTATCCCTCCTCTTATTGCGATCGAATCCTTTACCTTTGATAAGGCAAAAGCACAAGGAGTATCGCTAGCAAACAATAATGAATATCTCGGAAATGTGAATTTTAATATTTATGGTAGAGGGATTTCTGCAGAAGATACGGAACAGATTTCTCGCTTGTTAACTGAGAGTTGTTTTGGAGAGAAGTCTTCTCAAGCTTTAGATGCGAGTTGAGCTCTTGCGGTGCTCCAGAAGTCTTTGGATCAAATGAGTCAGGTTTGACAAGCGGATGTGGAAAGAATGGCAAATCTTTTGGAGCTTAAGGATACCTTATTGCAAGAGGGTGCTTCATATGACTCTTTGTCTCCTTATAATAAGATGATCAAGAAGTTTGAGTTTTATAGAATGCTCAAAAACATTGGGCTTTGTAGAGGGTAAGATTATTGGAGAATCTTTTTCTTAGGGCGGTTTATTGACCGTCCTTTTTTTGATTTTATTTTCACTGTATGAAGGTCTCTAGCGTTGTTATTGGAGCGATTAAGAGGCATTTTTCGTGCTCAACTTTTAGATTGTTTTGTTTGCAAAATTCAAGCACCTCATCATCAAAAGTTCAAGGCTGAAACCAGACTTTTTTGATTTTTAGTTCTACGACTTGAGGAAGGATTTGTAAAGTCAGTTTCGGATTGACCATAAAAATCACGACTTCGGGAGTAGTTTTTAGCTCGGAAAGAGAAGCAAAGCACGGAGCTCCAGCTATCGTTTGATATTTTGGGTTGATGGGAACGACTTCAAAGCCTCCTTCTCTTAGGCGAGTAAAGAGCCTAAACGCAAGGGATTCTGGATTCTCAGAGGCTCCAATCAGGGCAAGGGTAGACATAAACTGCAAAAAAAGAGTAAAATTTTGACAAAATCCTATGGGGGGGTATAATGGAGGTAGAAAAATTATTTACTATTTCTTATCTCATGATTCAACATTATTTCTTTAACGCACTTGAGAATCCTCAGACACTTAGTCTCTTTGGGAGAGTATTAGAATCTTTGTTTTGGAGAGCTTTTTCCCTCGTGGCATGCAGTATTTTTTTACTTTTTGTTTGATATAGTGTCTATCTACTGATCAAAAAGCATTCTCTTGCTTCAAGGAGAAAGAATCTGACTTTATCTGAGTTAAAAAACTCATTTCTTCTTAGCTATGAAAAGCGAAAAGGCTCTCTTTCCAAGTCTCGTTATCTTATCCTTTGAGCCGTGCTTCAACTCCTAAGTTGGAGTTTTGTTCGTTTTATCTTTGATGCAATGTCTCAAGGACTAGAAACCTCAAATACAAATGGACATTTTGCTATCAATATGCTTTTGGAGAAATGGGCATCTTTCTCTTTGTTGATCTTTATGATCGCCCCTTATTTTATCACGTTTTCCTTTGGGAATAAGCTCGTTCGTAATTTAGGGATACTGACCTATATTTTTTGAATCTGATTTTTGTTTTTTGGATTTCTCCTTCAACTTGGAACGCTTGTTTAATAAAAAAAGAGGCTCTTGTTAGAAAAAAGGATTTTTTAATTATGCTCAGATAATCTTATCAATCAAACCGTAATTCAAAGCATCTTCTGGGCTCATCCACTTATTTCTCTCCATATCAGCAAGCGCTTCATCATAGCCTTTCCCTGAATGCTTCGCGATGATTTCAGTCAGCATTTTTTTTACTTTGAGCATCTCTTCAGCTTCAATCTGAATATCGGTCGCCTGTCCAGAAATTCCTCCTCCAGAAATCAAAGGCTGATGAATCATAATCCTAGAATGTGGCAGAGCAAACCTTTTCCCTTTCGTTCCTCCAGCAAGCAAAAGCGATCCCATAGAAGCCGCAAGTCCGGTACAAATAGTTTGAATATCACACTTGAGGATCTGCATCGTATCGTAAATTGCCAGTCCTGAATAGACTTCTCCACCAGGCGTATTGATATACATGATGATATCTTTCTCAGGATCTTTTTTTTCTAGATAGAGCATTTGAGCTACGATTGTATTCACCATTGCGCTATGCACCTGCTCTCCAACAAAAATAATTCTATCTTCAAGGAGTCTTGAGTAAATATCGTAGGCTCTTTCTCCATTTTTGGTTTTTTCAAGGACGGTAGGAATCAGGGTCATCAATTTCTGTAAAAGATAAAAGTCTGATGTCTTTTTAGTGTTTTGGAAAACGATTGCAACTTAAAAGTATTTCTCTGGCTCCTTGCTTTATTTTTGTGTTCTGCTTTTATGAATTTTTTTTGGTGAAAGGTTCTTTTCTGTCGGATTTGTTTTGACTTGTAAATGAGTGATGCTTCTTGCCTGTCATCTAAAATCAGATTTTCTACTTGAAATTTGGAGAGAAAATCATACTAGTAAGTATTTGCTTTCTTTATTTTCTTTCTACAAAAAATGAAAAAAATCTGATTTCTTTTAATGGGAGCTACTCTTTTACTCTTGAGCCCGTCGATTTTGGCGGCGAACGTTAGCCAAACTCAGGTGAAAGAATTTTCAACGAAGTTGGAAAATCATAAAAATAAGCTTCTTAAAACTCTTGATGAGAGTAAGGTAAAACTCAAAAAGGTTTCAACTGAAGCTGCAAAGTTTGCGGATACGAGTCTGTTTAGAGCGGCAAGTTGTTTGGGGGCGATTCCTGCTGAGGATCAGAATTTAAACTTTGATAAACTTGCTGATGCTTTGAAAACTTCTATTCTTAATGAATATATTAAGTTTGATGGGGATATTAAGAGATTAGGATTTGGAATGGCGGAGCAGGATCCTTTGATTTTTGGGAATACTCTTGATACTTTTTATAATCAGCAAGCGCTGAAAATTAGCTCTCTTGAGAGTGATTATTATCTCAAGACTGAAAAAGTGAAGAAAACTTTTCTTGAGTATGTAGAAAATAATAAGACTTTGCTTACAAGTTTGGCACAAAATCTTGATTATTTGGATGCTTTTCAAAAGACTGTGAGTGGAGTGACCCTTGCCTTTAATGAATTTAAGGCTACGGTAGATAGTCGTTCTGATCTTTTTAAAGCAATAGAAAAGGAAAAGTCTGTGCTTGAGAAGAGTTTTTCTGCTGAATTAGAGTCAATCTTTAATCAAGCACTTGTAAAGAATAAGCCAGATGATGCGACTCAGGCTAAGTATTTGATCCATAAAGATAATTTTTTGAAGAAGTTTACAACTGATATTCAACAGTCAGAATATTATCTTTTTTCTGCTTTGTTTTCTTATGCTGATTATGCAGATATTATGGGGAAAAAATCAGATCTTGAGAAAAAATTCTACTCTGCTTCTTGAGCTGTAAATTGTAATCTTTTGCTAACGACAAGTGCTAATATTGGGGGATATATTGATGGCACAGAGGCAAAGGCAGAGCAGTTGAAGAGAGGCTTGAAGACTCTTACGGATGCGATAAAAAATGGAAGATTAAATCTAAAAATGCTTGAAGAACCTGCACTTTCTTACTTTAAAGATACAAATGCGCAGATGGCGAAAAAGTTGCAGGCTAATTTTAGGGCTATGCTTGATTCAGAAACTCCTCAGTCTATGTTCACTTGAAATCTCAGTCAAACAGGACAGCAGGTTTCTTCAGAAGGAGCTTTGCCTGTGCAAAAGGTGACTTTTACTCAGTCGTTCAATAAGGGACAATATCATGAGCAGATAAAAACTCTTCAGACGGTTCTTAAGAATCGAGGATATTATCACTCTGTTATTGATGGGGTATACTCTCCTGCTACTATTGAAGCGGTATATCAGTTTCAGCTCAAAGAGGGTATTGTGACAGGAAAGGAGAAAAATAAGGCTGGTCATGGATGGTTTGGGCAAAAAACGAGGGAGAAGCTAAATAGTCTTTTGTAGTATTGGGGTACGTTTGTTTTTGCATTTGTTTTTTTGAGTGATGATGAAAAAGAGAGGATTTACCTTGCTTGAGGTGACGCTTGTGATCGTAATTTCTTCGTCTATTTTGACGACACTTTATGGAATTATGACGCTTTTGCCCAAAGTAAAGAATACGAATGATGCGAGGCAAAATCTGATTCAACAAACGAACGATGTTATAGATAGTTTCGCGAGAATCTTTCAGGATTATACTATTGATTATGAGGAATATTTTAATAGGCAAATGGTCGGATGCAATAGTAACAAAAGAGGAGAGAATTTTCAGTGGAGCTTAAATGCAAGTGGGCACTGTAATGAATTTACGGCTTATGGAAATGAAAACTCTTCCAGAGTGCAAACTGATGAACTGAAGCAGCATTTCCTTTCTTATTGTTCTTCAAATGGAGTACAGTGGCTTTTGAATTTTGTGAATAATTGTCAATCTGCGAATCTTCCTAATGATCGTAATCTGGGAAGGCAGTCTTATGGGCAATATAAGTGGCTCTTTTGGGATATTAGAGGAGATACGGATGGGCAGTCGATTAATGGAGTTACGGAGCTTAACCTTTCAGAAAAATATAAACCGCTAGAATTTGGGGATTCTGACGATAGGGATGAGTGAACTTGACCCGATGCAGTCTGAGATTCTGATGGAGTTCAGGAACTCTATTTGATTTCTCATGATAAGACAAGAAGGCTCTTTTTAAGAAGGACTTTGAAAGAATATAAGGATCGTAATGGCGTGGTTTCGACTGGATATACTATTCAGATTTTGAAATTAAGGGGCTTCGATGCTGGAAGAAAGCATGCGTTTAAGGCGGAAGAAGATTTAGAAGTTTATGATGGACAGGTGGATACTTGGGCGTGTGATTATGGACAAAAGTTTTCTTGTAAGGGAGATGATATTCCGAATTATGCATGATATAAGCTTCCTAAGGATAAAGATGATTGATGGGTCGATCTTTTTGATGATAATCTTAATATTGTCCACTGGAATATTCAGATTTCTCCTGTTAGAGATCCAGATCTTGCTCGGGCAATAGATGATGGGCAGATAAATCCTTTTATCAAAATGTCTCTTACTGCTCAGCTTAATCAGCATCTTTGGGCAAATAAGGTTTGAGGGGATACGAAGAATTTTCAATATACGCTTCAGAATGTCTATGATACGAAAGGTTTTTATCTCAGATAACTGATGAAATTTAGCTTATGCAAGTTTTGTGACCACAGAAACGTCATTCCCATTCGGGAGAATCTTCGGTTTTTCTTAGACCATTCCGTTTTCTTCAACAGCGGTTTTCTGATAAAAATTCAGGATTATTATTCTTTTTTTGTTTTCTCTTTTTTATCTTAACTCTGAGGCTTTTTTGGCTGCAGGTGGTTAAGCATGATGAATATGAAAGACAGCTCAGTAAATTGCACTATAGAGAGTCGCTCCTTGATCCTGAAAGGGGGAATATTTTTGCATTGGATAAGGGAGGGCATCCTGTGAAATTAACCGAAAATTTGACACTTTATGATCTGGCACTTGATCCTAATGATTTGACAGTTTTCCCGGAAAAAAAGCAAGATGGGCACGTTATTTCGCCAGAGACCCCTATGAAGCCGCGTTTTATTGAATTAGTTGCTCCAGTAATTTACAAGCATTTGTGTGAGTTAAATGGGATGAAAGTCCCAACCAAGGCTGAATGTGTCAAAAATGTAGAACTCTTTACGAATGTGGATTTACTTCCAAAACAGCCAGAATTATTTTATTTTTGAAGTGGAATTAGGTCGCCTGAGTATGAGACATTTGACTTTACAGGTTACCAACAGCAGTTTGATAAAGTCGTTGAGCAGTTTACGAAAAAGAAAGCTATGGAGCTAATTACCACCAGGCTTGATGAAAAAATTCAGATAGGAAAAAAAACCTCAAACTATCTAGGGTATTTTGTTGAACCTAGGTTTTTGGAGGAGGTGAAGCAATTGAATCTGCCTTATCTTACTATTGAAAAGGAGAATTATCTGTATATTGTGCCACTGAATGTGCAGAATAAAACGCAGGCGATTAATCAGGTGAAATCTTTACTGAAGAAATGGAGATATCCGATAAGTTCAACTTTTGATAGTCTGTTTGAAGTGCAGGATTGGAGATATATTAAGCTCATTTCTTGACTCAATCCTCAACTTGCTCAAGAGATTAGAGATCTTAAAGTTCAACATCAGTTTGAAAAATCGAAGCCGAATATAGATGCTAATGTGTATATTGGTATTCCTGTTTTGTACGGACTGATTTTGGAGCCCTTTACAACGAGGTATTATCCTTATGGAGAGTTTATGTCTAATATCTTGTGATATGTAGATAGGAATGGTATTGCTTATTATGGAGTAGAAGAGTATTTTGATAATTTACTGAAAGGAAAGAGAGGAGAAATCAGATGAAGAACCTCCGGAATGGCTGGTAATGTTTGAACGAATGAATTTGAAATCGTTCATCCTACTGATGGATGAGATATTTATTTGACGCTTGATATTGGGCTTCAGAGAGAAGCAGAACAAATTGCTCATGAGCAACTTCAGCAATTGAGAGCAGATGCGATTAGTATCCTAGTTTTGGATGCTGAGAATGGGCAAGTTAAAGCTTCAGTTAATGCTCCGACTTTTAATCCTAATAATTATAATGATGCTTATACCTTAATGCCATTAGGACAGGAATATGCGCATTTGATTGATAATTTGAGCTATGTTGATGTGCCGGTTTATATTTTTACGGGAAACGATTATAGGATTGCTACCTTATCCGAAAGGCAGAATACTGGACTTCAAAAATTTATTAACACGAATGTTTTTTGACCGCAGGTTTTTGTAGATAAGAACATTAGTAGTCCTTTTGAGCCGGGAAGTATTTTCAAGGCGTTTACTGTCGCTCTCGGACTGGATATGGATGAAGTTTCTTATCAAGATACGTATCTCGATGAATGATCGGTTCAGGTTTGAGACTATGTCATTAAAAATGCTACAAAAGTTTGTGAGGGACATCATACCTTTCTCCATGCTCTTATCCATTCTTGTAATATTGGAATGGTGAAGATTGTGCAGAGAATACAAAAATATGCCTTTTATAATTATCTTACGAAACTTGGTTTTGGATCATTTACTGAAATTGAACTTGCTGGAGAGAAGGAGGGCTTCTTGGATAATGTAAATCTCGTTTCTGTGGCAAGATTTTTGAATAATGCTTTTGGTCAGTGATTGACTGTGACTCAGATTCAGCTTGCAGTTGCCTATGCTTCTTTAGTAAATGGAGGAGAGCGTATCAAACCAACTATTATTTCCAAAATTATTAATAAAGAACAAAATGGCTTGGAACAAGAAGTTTCTTTGATACAGGAGAGAAAGCAGATTTTTAAGCATAAAACTTCAGAGCTCTTGAGGTCTTGACTTTGGGAAGTGATGAATAATAATCCTGAGGTTTATAATGGGGCAAATATTCCAGAAGTGAGACTTGGAGCAAAGAGTTGAACTGCGCAGATTTCTTATCGTGGGAAATATAAGAGAGGAGCTGGTCGAACTAATGGAACCTTTGCTGGGGTAGTCTCTGTTGATGAGCCGAAATATGTGATTTTGATTTGGGTGCGTCGTCCAAGGACAAATCAATGGTGAGCCTATACTGCTGGACCTATTTTTAGGCAGTTGGCTTCCTATATTCTGAGCTATGATATGTAATTTTTATTTTCTCTTCCCCTTATGAATACTGTTAACCTTTCAAAATTAGTATTGAGTTCTTATTTCTTTGATCTTTTGGCTCAGGAAAATATCCATTTTGAGCAGATTTTTAGTGATAATCCTGCTTTGGCTGCTTTTGGGGTTTCGGTGATTGATAGAGATTTGAGCACTTTTTTTGAGAGGCAATCAAAGAAAAAAAACTGACTCATTATTTTGGATCAGCTTTCTTTTTCTGATATTACACCTTTTCTTGGGCAACTTTTTGAAGATGTTATTGTGATTGATGCGTGGACGGGAGTGTCTAGTTTTTGTAGAAAATTTGTTCCCGAATTACGTTTTTTGGAGGGGGCAGAGGACGTCGTATTTCCCCTTGATCTTCAGTCTTTTTTACGTGCTTTAACAACATGTAAATCGGCTTTAATCCCTCTTACTGCTCAAGAAATTCCCGAAAATATTTATGAATCTCCTTCTGAAGAGGTTTTTCAGATTGTAGATAAACACTTGGTTGATCATTCTGATTTTCTCCCTTTAGCTACTCCTGATCATCCGGAGCTTCTTCTTATCGGTATGGGAAGTTATTTTGAGGAGTATGTAAAACTTTCTCAACTTTTGGATGCTTGCCCAGAACGTATTGCATTGGGTGTTTTGTGAAAAACTTCGGCTTTGGGGCTTGATTCTGTGCGGCGTTTGGTAAAAAATGCAAAAAAGATTGGAATCTGCATTGATCACGAATCTCTATCGGCACTTGCCTATTTTAGGAGATCTTTGCATGATAAGCCAATCTTTATGATCACTCCTGACTATAAGAAACTTACAACTTTTGCTCCGGAATGGCAACTCGAGCAGGTGAGTTTTGATGCTTCCTCATTATTGGAAAGGAGTTTGGAATTGCTGGTTTAATTTTTTTAATTTATGAAGTCTGCATCTGCTCTTCTGTAGTTGAAGAGTTCTGAATCGTTAAATCGATGTCTGATTTCTTTTTCTGCTTCCTCACTGTCTCCTGATGCATGGATCACATTTGGGACTCCGCATCCTTTTTCGTTGGCATAGGCAAATGACATATGAGAAAAGTCTCCTCTAATAGTGCCTGGTGCTGCAGCTTTTGATTCTGTGGTTCATGCCATTTTCCTTACGAGGCTTACTGCTTCGACCCCTTCCACTACCATCGCTATAACAGGGCTTTTTTGCATGGCTTCTAGGTTAAGGTTGAAGGTCTCTTCTCCACGACGAGTGATCATAGTTCCAATTGTTTCGTAATGATGATGGAGGAAATCTCTGTCAGGTTTTTTCATCTTTATTCCTACGATTTTGAGTCCAACCTGTTCAAAGCGGGTTAGGATTTGACCTACGATCCCGCGACAAACTGCATCAGGTTTGAAGACTACGAGTGTCCTTTCTGTGTTCATGGTGCTTGAATTATTTTGAGGATAAAATCAGATTCCTTTTATTTTTTAGACGGGGGATTACAAGAGATTTTGTTATTTCTTCTAGACTTTTGGACTATAATCGCTACAAGTAGGGAAAAGTCGGATTTTATTTCTTTTTTCTCTAAGGATGCAATACTGATTCCTTGCCCTGATCTCGCTCCTGGTTGCGACTGCTGGACTCTTTCTTTTTAGAAAATTAAAAGTTTTGGATAAGCCGGGTAGTGATCTCAAAAATACGAGAAAACCAGTTCCTACCTTACAAGGAATATTTGTGATTCTTGCCCTCGTGGCGGGAATTGGCGTTATTTATCCTACTTATTTTTCTGAGCCGCTTTTTCTCGGGCTTCTGATTCCATGACTTTTGATCGGTGTGGTAGAGTTGATAGAAGAGCTTAGCTATCTTGGGAAATTTCCAAAGATTCCTCCGATAGTAAGGCTTGTCGTTCATGTGTTGGCTGCATTTTTGGCGGTGTGGATCGGTGGTTTGAATCAGCATTCTGAATTTGTTTTGTGATCTTTGGTTTTCCCGATTCCGAGCTGGATTTTTACGCTGGCTTTTGTCGTCTGGACGATGTTTTGTATTAATGCAATTAACTGGTTTGATGGAATTTACGCACAAGGAAGTGGAATTTCAGCGATTGGTTTTTTGACGATTTATCTTGTGATAAAATTTGTAGTTTTCCAGAGTTATGAAACTTTCCCTAATATTGAATCGCTCCTCTTCGTTCAGAATTTATCTTTTTTGCTTTTTATTATTAGTCTGATTTATACGGTGATTGAGTACAAGCCGCTCTGATTGGTGAGGGACGTATGAATTATGTTTTTTGGGTTTGCTATTGCTTATCTTTCTGTTGCTGGAGGAGATAAGATTGGGACTTTGGTAGTGGCGCTTTCTCTTCCTTTGTTTGATGCGGCTTGGGTTGGGCTTTATAGGATTTTTGTGCTTAAAAAAAATCCCCTCAATGGGGACTATACTCACTTTCATCATAGGCTCTTGGGATTAGGATTTTCGAGAGGAGAAGCTAGGGCTTTTATTCGGATATGGTCGCTTATTATGATGGTTCTGATGCTTCTTCAAGGGACGAATAGATTGCATAAGATTGTTATTTTTCTGCTGATGGCGGCGTTGTTTTTTTGAGTTAATGCTTATCTTTTTCTCTATAAAAAACTTCCTTGTGGGCTTCAAATTAAAAAAGAGAGATAATTCTGATTTTCTTTTTCTTTATAATCCCATTTTCCTTGCGAAGTAGGCTTCCTTATGCTCAAAGCTAATCGTTAGATGAACTGCTTTCCCAAGCTCTTCACTCAGGATTTGCTCTATCTGTTTCCTATTTTCTTCGTTATTGAGTAGCATCTCGGCAATTTTTGTGATGACGGTGATCTGGATTTCATCGTTTTCAATCTGCTCAATGATGGCTTGATCTTTTAGGTTAGATTGGACGGTTGGTTTGGTGAGCTTGGAAATTACTATTGCTCGCAGTTTTTTGAAGTTTTCCTCTGAAGCTTCGGCTCATACTTTTTTTTCTTGAGTTGGGGTCTCTAGTTTTTGCTCTTCTGGTGCTGGTGTTTGCGGTGGAAGAGGAGTTTCTGTCGGTTGAGGAGCCTGTTCTACTGCTGTTGCTTTTGCTGGTTGAGGTGTTGGTGCTGTCGGTTGAACGTTTACTTCTGGTTTTGGATCAGCTTTTTCTTCTTTTTTGTTTTCAGTAGGTTTTTCTCCACCTGTGCTTTGAGATTCCTTCCCAGCTTCAAGGGCTTTATTGATTGCGATTTTGTATGCGATGAGTGGGTATGGGTATCGCCTTAGGGTGCTTAGTACGTTTCCGAAGATTTCTGAACAGTGGAGCAAGAAATCGGTGTCCTCAAGCAGATGCTGGTCAATGTAGGAGATCGCTTGTTTTGCGAATTGTCCGAGGTCGATCCCGTGCTGGTTGAGTTCGTCAAGCTGAGAGAAAATCTTTTCTCTTTCTCCTGTTTTGATGGTTGAAAGTAAATTTTTGATCGTTGTTTCTCCTGCTACTCCAAGAAATCTTGACACATTTTCCTCATTCAAATCTCATAAAATGCTGACTTGATCTACGTATTTTACTGCGTCTCTCACGCACCCTTCTGAAATGTTGGCGATGATCTCAAGTGCATTTTCTTGGTAGTGGAGCTGTTCTGCTTGGCAGATCTTTTCCAAGTGATTGATCATTGCTTTATTTGGGACTTTGCGGTAATTGAACACTTGGCAGCGGGAAATGATGGTATCAGGGACTTTGTGGATTTCCGTCGTAGCAAAGATAAAACATACGTTGTTTTTTGGTTCTTCGATGGTTTTTAGGAGTGCATTGAATGCGCCCTTACTGAGCATATGCACTTCGTCGATCACGTAGATCTTTTTTTTCAGAGCGGTTGGTGGATAGAGTGCTTTATCAATGATTTCCTCTCTGATGTTATCTACTCCTGTATGAGAGGCCGCATCGATCTCCACATAATCTAGGCTTGTTCCGTTATTGATTAATTCACAATTTGCACAATGATTACAAGGGTTTCCTTGTTGAAGGTTGAGACAATTGAGTCCTTTTGCAATGAGTCTTGCTGATGAGGTTTTCCCGGTTCCTCTCGGTCAAAAAAGGAGATAATTGTGGTGGATGTTATCTCTCACTTCCATTTGTGCTTTGAGGATATTGGTGATATGTTCCTGTCCGATCATTCCATCAAAGGTCTTTGGGCGGTATTTATTTGCGAGGCTCATCTTGATTGTATTGTACAAGGACTAAAATTCTGATCCTAGTTTAAGGTTTCGTCTTTTTTTTTCAAAGGGAATTCATCTTGAGCTTGAGAAAAGATTTTCTTCCCTATAGAATAAAGGCATTTCTCTGGAAGAGATCAGCTTTTTCTTTTTGCTTTTTTATACTTATTTTCTGGAGGGAGAGATGATCTTCAAAAAATCTCTTGATTTTCGTCCTCGTTTTCATATAAGATGTCGTGTCATCATAGACATAGCCTTCTGCGGATATGATGTAGTTGGTGTAGCATAGCAGACTTCAGCTCTGTTTGTACGGGTTCGAGTCCCGTTATCCGCTATTTATCACTACTTGTATTCTGGAATGCTTGGTCGTATCATTTGAGGTTTGGTTTTGGCTGCTATCGGAGGTGCGATGTGTTATTATTCTTATCAGATTGTTCAGATGTTCTGAAGGAATGATTGGGCAGAGAGGACACTTTGAGGAACAAGGAATTTAGTTGTCTTCATTGGTGTCGGTGCGATGGTGATTTGAGTATTGGTGATGTTTGGTGTTTTTAGTCTTTCTAATCCTGCTGATCAGGTTCCAGGAGGTTTTGGGGAGGAAGGTGCCAATTCATAAGTGTCAATGGGCTCTTAGCTTAGTTGGTAGAGCGATCCGATTCGTATCGGATAGGTCAGCTTGCGAGGTTAAGCCCTAGAGAAAATATGGGCTCTTAGCTTAGTTGATAGAGCGATCCGATTAGCATCGGATGGGTCAGCTTGCGAGGTTAAGCCCTAGAGAAAATATGGGCTCTTAGCTTAGTTGGTAGAGCGATCCGATTAGCATCGGATAGGTCAGCTTGCGAGGTTAAGCCCTAGAGAAAATGTGGGCTCTTAGCTTAGTTGGTAGAGCGCCTGCTTTGCACGCAGGAGGTCAGGGGTTCGACTCCCCTAGAGTCCAAATTTGAATTAAACTTTTAAAAGAGTTTAATTTTTTTTAAAACGAAAAATAAAAAATGAAGTGATGGCTATACATTCTCCTCCTCGCAAATGGGCAATATTATGTAGGAAGCACAAATAATTTAGAAAGGAGGATCGCACAGCATAAGTCCTGAAAAGTACTCTCGACGAAATGAAAACTTCCTTTGGAGCCTCTCTTTACGAGAGAATATGAAACTTTGTCTGAAGCAAGAAGTGCAGAATATCAACTAAAGAAAAAGAAAAGTAGGAAATATATTGAGTTATTTATGAACGCTTAGTAGCTAGAGCGATCCGATTGTCATCGGATAGGTCAGGGGTTCGACTCCCCTAGAGTCCAAATTTGAATTAAACTTCTCAGAGAGTTTAATTTTTTTTGATTTGAGAAAATTCTTGAAGAAAGGTTCTTCTTTATGACTTGTAATGTCACGGAAGTTGCTAGAATGAAACTTCTTCGTTCTCCTTGATTGAAATACGAAGCTGCATTGTTATCTTCTTTGCTTATGGATTAGGATAATAGGATAAAAAATCGCTTCAAAAGGCGCTTTAGTGCTGGAGATTTCCTTCCTCTTGAATCTCAATCTAAAAAGGGAGAAAAATCAGATTTTTTTCTTCTTTTTTGTTGTTGTGAAAAAAGTGTCGATGAGAACTGGAAAAACTTTCCTTGAAAAAAAAAAAAACAATTCATTAAAAAACCTTTTGAGCATTTGTCTCTTTTTACTTTCTAAAAGTCAATAGCAATGATGAAGTTTAAGTTGATTGAAAAGGTAAACCCTCTAAAAAAAGAAGACCCAAAGAAGTGGTATGCAAGTCCTATCTATGCGGATAAGGTAACACAGAAAGAGCTGGCAAAGCAACTTGCGGACCTCTCTTCACTTTCAGCTGGAGATGTTGGGAATGTAATTCAGAATCTTGTAGAGGAATTTTCCAAGATTCTGATGAGGGGAGGTATTCTCCAGTTAGGAGATTTTGGGACTTTTCGTGTAACTATCTCGAGTAAGGGAGCAGAAGAAAAAGGTAAATTTAGTGCTGAAACGATTGAGCCAAAGATTAGTTTCTTGCCAGGAGTAGAGCTCAAAAAGCAGCTTGAATCTATGAAATTTCAACAGCAATAGCTTGAAGAACTGAGTAGGGAGGTCTATTTTTTGAGAGGATATGGCTTGGTAGAATTTCCTTGTGGAGAGGGAGGAAGACTTCACCGTAGAGAAAATGAAATCTGACTGTAGAGAAAAATAATTCTGTCTGGAGAGATAGAAAAATCTGTATGGTCAGATATTTCTTTATCATTTAATGAATTTTTATGCCAAGAATTAAATATGAAGAAGAATGTAAATTTAGAGGCATTCTTTTCAAGAAGGGGGAAATTTTTTATGCGGATAGGATTCAATTTAAGTGGGGTTGGCGAGAAACTCGTTGGCTAGTATGGTTACTTGCTTTTGTTATTGATATTCTCCTCTTAGGTAGTGGAAGATTTCCTATCTGTGTCCTAACAAAGTTTTTATTTCCTTCGTGAAAAGAAGCCCCATGGAAATGTCGTTTTTTTATTAATGAAGAATGTTTATGATGGAGATATTGTCCTAGGAAATCATTTAGTAGTGAATTATCAACTGCTCTCTTAAAGAAGGGTAAAATTGTTGAAGTACAATTATTTAGACAAAAAGGGCAAGTAAAGGTATATAAAAAAGGAGTATTCTCAGAAATGTCCTGCGTTGGTAATGCTAGAGATATAGGGAAGATAGATGATCTTGCGGTCCTCTTGAAAAAATATGGATATCCTTTTACTTTTATGGAAGAATAAAAAAGAATTAAACTTTTTGTTATTTGCTATTTAAGTTAGGAGATTGTTATTTTCTTTCTGTAGGGAAATGAAAATCTCTATCCTTAGATTTTAAATTTTCATTTGTAAAGTATTTCAAGATGAAACTTTTCTTTTTTGATACTGAGACTACAGGAGTAAACCCAAATAAGGATAGGATTATTCAATTTGGAGCAATTTATGGCGAGTATGATCTACAAACGGGACAGTTTAATGAGTTAGAAAGAATTAATCAGTATATTAATATTGAAGGAGAGATACCCTATTGAGCTAGTCAAGTGCATGGTATTTACAAAAAAGATATAGAAGGTTATGGCTATATGAAAGATTATATTGATGAATTTTTGGTTTATATGGGGCGTGCGGACTATGTAATAGGTCATAATGTAGATTTTGATAAAAGGATGTTTATTTCAGAGGTAAAAAGACTTGGCAATAATTTTAATTTTGATGCAGTTCGTTGGGTAGATACGATGAGGTCGACTGCTCAATTTGTAAATGAGAAAGGAGGAAAGCGACCTAAACTCATTAAGTTGCATGAAAAGCTCTTTGGTAGGGGGTTTGATGGGGCTCATGATGCAATGGCTGATATCGTAGCTACGAAAGATTGTTTTTTGGAATTAAGGAAATATGGCTTTTTTCGTGAAATTTTTAGTTTACCTTTATGAGGAGGAGCTTTGTCTCGGGGGAAAAAAGTAGAAGAAAAAAAGGTAGAAAGGAAAAGGGTAGAAGGGAATAAGAGAAGTTATGATGAAGTCAGAAATCTTGAAAATTGATATGCTCAAGTTCGTATATGAGACAAGCGAGGGATTATAAATAGGCGATGAAAAGAGGGGGGCGAAATAAAATATGATACAATTGGTGATTTTCGTGATTGATATGCTCAAGCTCGTATATGAGACAAGCGGGGGATTATCAATGGAAAATGAGAAGAAGTCTGTAAAATCCAATATGATGCAATTGGTGATTTCCGTTGTTGATATGCTCAAGTTCGTATTTGAGATAAGCGAGGGGTTATTAATAAGCAGTGGAAAGAGATTTGTGAAATCAAATATGATGCAATTGGTAATTTTCGTGTTTGATGTGTTAAAGTTTGTATATGAGACAAACGAGGAATTGTCAATGGAAAATGAGACGAGGTCTGTAAAATTAAATATGATGCAATTGGTGATTTTTATGATTGATATGCTCAAGTTTGTGTTTGAGATAAACGAGGGATTATAAATGGGCGATGAAAAGAGGGGTGTGAAATTAAATATGATGAAGTTGGTGATTTTTGTGATTGATATGCTCAAGTTCGTGTTTGAGATAAACGAGGAATTATAAATGGGCGATGAGACGAGGTCTGTAAAATCAAATATAATGCAATTGGTGCTTTCCGTTATTGATATGCTGAGGTAAGGGTTTGAGACAAGCGAGGGATCATTAATAAGCAGTGGAAAGAGATTTGTGAAGTCAAATATGATGTAATTGGTGAATTTCGTGATTGATGTGCTAAAGTTCGTGTTTGAGGCAAGCGAGGGATTATAAATGGGGGAGGAGAACAGCTCTGTGAAATTAAATATGATGCAATTGGTGATTTTTGTGATTGATATGCTCAAGTTCGTATTTGAGAGAAACGAGGAATTATTAATGAAAAATGAGTAGAGATTTGTAAAATTAAATATGATAGAATTAATATTCCCCCAAGTTGAAAGAAACTAGGGCTTATAAATGAGCAATGAGAAGATGTCAGCGAAATCCAATATGATGCAATTGGGGATTTTGGAGTTTGACATCTTAAAGTTCGTGTCTGAGAGAAGCAAAGAATTATTCGTGTAGGGTGAAAAAAGATTTCGGGGTCTTTGGTGCTATTTTTTTGGATCGTTATATTTCTTGTGAGTGTTATATGTTTTTACTTTTTTTACTAAAAACCAATGTGGGATATCTATAAACAAGTTGGATGATTAAAAAAACGGATTAAAAAAATTACAAACTATCTTCGCTTTTTTTTTGTGTTTTGTATTGTTTGAATACTATTGATCATCGGCATAGTCTTTTATCGTTTAGAAGGTAGATTTATTCTAGGGAATCGGTGAGCTACTCCACTTGAATGTTTTGAATATGGTGAAGTTTGGCGTCAAATTGTGACAGAAGACCAAGTTTTGGAAATTACAGGGTGGGGTATTTCAGATTATGATGTTCAAAAGTGTTGAAAAAAAGTAGTCATCCCAAAATATATCAAAAAAAGTCCAGTACTCTCTATTTGGCATTATGCTTTTTATGATAAATGAATAGAAGTTGTTGAGTTTCCTGAGGGGCTTCAGCATATTGGGGACTCTGCTTTTAAATTTAATCAGTTGACCTCAGTAACATTCCCCTCCACTTTACGAAAAATGGAGGCTGATGCATTTGCAGATAATAGGCTGATTTCGGTTTATTTACCTAAAAATTTGAAAACGATCCGTTCTTGTGCTTTTGCAAAAAACCGTATTCAGTCGCTTATTTTACCAGAAGGACTTCAGTATCTTGGTTATGCTGCATTTGCAGACAATCAGATTGCCTCCATCCATTTCCCTAATTCACTTAAGAGGATGTGAGCATATGCCTTTAAAAATAATCAACTAAAGACTCTGGTATTGCCTGAAGGTTTGGAAAAATTAGAATATGCTGTTTTTTCACATAATAAATTGATAGGTGTAAAGTTCCCACAAGACCTTAGGAGTATTGGAGCCTATGCTTTCCATGATAATCTCCTTACGGTGGTTAATATCCCTCCAGGGGTAGAGGAAATAGGGACATGAGCATTTATGGGGAATGAATTAGTAAGATTGGATCTTCCAGATGAAGTAGAAGTTGTGCAGCCTTATTCTTTTGCTAAAAATAAGATAATTAGCCTCAATTTCCCTACTTCTGTGGAAGAGATTAAAAAATGAGCATTTCAGCAAAATCAATTGTTTTTTTTGAGTATCCCACATACTGTTAAGAGAATAGAAGAGGTGGCTTTTTATAATAATTATTTGGAAGGGGTATCTTTTTTCTCAGGACTAGAAGAGATTGGTTCTCAAGCTTTTGGACAGAATAATCTTACTTGAGTCGAATTTCCAAAGTCACTTAAAAAAGTGTATTATAATGCTTTTGATTGGCAAAAAATAGAAGACTTAAAACTTGCAGAAGAAGGTTCTTGAGTCTGTATTGATCGAGAGATGCATGACAATGATTATGTGTCTGGCCATATCTTAGAACAATTAAGAAGTGTTCCTTCTTGTTCGAAAGATAACCAATCTGAGGATTTTAGTAAATTTTAGTATGGTTAAGGGGGATTTTGCTAGTATTATTCGATAATTTTTTATTAAAGGATTTGAATTAGAAATATTTAAGGTGTTTTTTATTCTTTTAGTTTTATAGTATTTTATCTTTTTTTTATTATTTTTTGTTGATGAGTTAATATGTGATTTAAAATAGCACTAATAGTATTTGTATTGCTTATTGTAACATTATATTATTGAATAAGGAAGCGAAAAGAAAAAAAAGCTGATTATCAAAAACAATTACAGCAGGTAAAGCAAGCAATAGAAGAATTTATAAACCACTTACAAGACAAAATACATTGAAAGTACTTAAATTTTTCTGCTTATAGGAATTTGTATGACGAATGAGTTTCTCTGTATGATAGCACAAAAAGGTATCATAAAGATTTGCAAGAATATTGATTATTTTTTTCATTTGTGGAGGATGTAAAATTTAGGGATGATCATAATGATGCTTTTGTAAATCAGGAAAAAAAACAATATGAAGGTTATTTTAAACAACTAGATGACAAAGGTTTTTGATTAACTGATAAACAATTAAATGCTATTTTTACTGATGAAGATGCTACTCTTATTAATGCTTGAGCTTGAACAGGGAAAACAAAGACTTTAGAAAATAAAATAGTTTATTTGCATGTAATAAAAAAAGTCCCGCTTCAAGATATTCTGGTTATTACTTATTCAAAAAGTTCTCAACAAGATATGTTAAAAAGGATTGTAGATTCTTTAAATAGGGCTTGAGTTAGTGTACATGAAGATGCACTTAAGTTTACCGTTTCCACATTTCATGCATTCTGAAAAAGGATTCTAGATCAATATTATGCACAATGTTGCTGGTCATGAAGTGGTGAAGAAAAATTAATTTGAAAGTGATTTGTTGGTAAAAAAGTAATAGAAGAAAAAGATCAGATGGAAGTTATGGAATTGGTTATGGATAGGTTGAAAAAGGATGAATTTTTACAACCTATATTATCTAAATATTTACTCTATTTTAGTAGTCCTGAATATTCAGTTGAAGAGTTTGAAACGCTAGATGACTATTATAACAGAGTTAAGAGGTCTTATCTAACTTTGATTAAGGATTTTAAGTGATATAATGTGGTTGTAAAAAGTTATTGAGAAGTTCTTATAGCTAATTTTTTAGTTTCAAATGGAGTTGCTGTTGAGTATGAGCCTAAATCACACTTTTACACAACAGATACTTGAGATAGAAGAACTTATAAACCAGATTTTTATTTGCCAGATTATGGTATTTATATTGAATATTTTTGAATAGATAAAAATTGAAATACGGCTCCTTATATATCGGCTGAGAATTATGTACATAGGATGAATCAAAAAAAAGAAAATCATAAAAAATCTTGAAATAAATTGATTGATATGAGATATGCTGATTATCAGGACTGAAAGTGGTATTTTCTAAATAAATTAGAGAAAGAATTGAAAAAATATGGTGTTCAGTTGCAACCACAATCACAGGGAGATATCATTAAATTATTAAAATGACAATTATCATGATTGGATAATATCTTAAAAACTTTTTTAGCTCTTTATAAAGAATCGAGAGAAACGATTGAGGCGCTAAAAGAAAAGGTGCAAACATTTGATGGATTAAATAGAGAAAGAAATCAGATTTTTTTAGAAATATTTTCTCACTATTTTAAGCAGTATACAGATCTTTTAGTGGAATGATGATTTATGGATTTTTGAGATATGATATCAGATGCTAAAGATATCATAAAATCAGGAGCCGTTCAAAAAAAATATTCATATATACTAGTGGATGAATTTCAGGATATATCAGAAGCTAGGGCGATGCTTCTAAGAAATCTGATTAAAGATCATGACGAGACAAGATTATTTTGTGTATGAGATGATTGGCAGTCTATTTATAGGTTTGCATGAAGTAATGCTAATATTTTTTTAAAATTTGAAAATTATTTTGATTATACTAAGAAAATTACATTAGATAGAACATTTCGTTTTAATCAGTGAATATCAGATATTTCATGAAAATTTATTATGCAAAATCCAGAACAGACGGTAAAGCGCTTATCATCAAATAATAGAGAAACAAAAGACAAAATATTGGTTTTGCAAAAGGATTGAGATCAAGATCACGTCTATCTCTCTATTCTTAATAGTATTTTTGAAAATGTATTTAGAAAAGCACCAGAAACAAAAGAAATTTCGATTATGTATTTAACAAGGTATTCTAGTAAAAAATATGCAAAAAAACATGATCAAGATTTTATAGATTTTTTGGTGTGAAAATATTGAGCAGATAAACATATTACAAAGGATAATGGGAAGGATATTTCATACTATGAAACTAGTAAATTAACATATAGAGACAAGAAGATCGCCATCAAATGTATTCCTTTGACAGTTCATAAATCTAAGTGATTGGAAGCTGATTATGTACTTGTTGATTATATTAATCAAAATGATGATTATAATTTCCCTAGTAGTTTTGATGATGATCCAGTATTAGAATTACTTTTAGAAGAAAAAAAATGAAAATTTATTTTTTCAGAAGAAAGGAGGCTATTCTATGTTGCCATAACAAGGTGAAAAAATTTAGCATTTTTGATTTATAATAAACAGAAACAATCTTTATTTTTAAAAGATTTATTAGCTATTTGATGAGAATGTGTGAAGATTGTAGATGTTGGATGACAGAAGCTAAGCCTTCTTTCTGATTATCAAGCTCCTAAATGTGCTAATTGTTGATGAGTATTAAAAGTGTCTCAATATGATCGTATATATCCTGAATTTTATTGTGCAAATTATTATATGTGATGTGATGTAAAATATTTTAAGTATGATTGAAAGTTGTATAAGGCTTTATTATGTCCAATATGTTGAAATTCTATGATGCTAAGGCAAAATTCTCGTAATTATTCTGTATTCCGATGATGTGTAAATTATCCAAGTTGTAGATGAACTAGGTATTTTCTTAAATAAACGACGATAAAAGCTTGTATAATTATTTAAGGAGTAAATTTTTATATATCCCCTCTAATCCTTGCTGTTTAGAAAGATGCTGATAAATTCTGTTGATCTTAGAGTATGAAAAGACTTTTAAAAAAGATTTAGTCTTAATTTTATTCAAATGTATTCTAAAAAATTAGATTTTCTTTTGCAGGGATTAGGTTTTTTATTTAACTTTTTAATATTGTAAAAGGAAAGGTGATAAAAATCTGAACTATGAGAATAGCTTTCCCTTTTATAAATCGGATAGATTTTGATAAAAAAGAGATAATTTATATCTATATTAATGCAATATGTATAATTAATGCAATATGTATGTAAAAATGCGATTTCGTATGTATATTGCAAAGATAAGTGTATTTTTTATCCTCAAGCATTTTCCAAAAGTGTAAACAAAGCTTTATTAAGATAATTTGTTTATAAAAACAAAAATATTAGATAAAATAACCTTCTTATCTAAGAAAACTGAAACTAGCTTTTTTGTGCCTTCCTCTTCTTCTACTTACTAAGCGACAAAAGTCAGACTTTTTTCTTTTGCTAAAATAAGCTTCTAGAGCCTAAGCGAGATTAAAAACAACCATTTTTTCTAAAAAGTGATAAAATAGCTAAAAAAACTCTTGATTTTGTATCTCATTTTCATATAAACTTGCACGATCATCTTTGGCTCCACTTTTGTGGAGTAGCTGGATGAGTGTTGGGGAGATGGCAGAGCGGTCAATTGCATCAGACTGTAAATCTGACGCCTCACGGCTACGTAGGTTCAAATCCTACTCTCCCCAAGTGGATTCGTTGCGGAAGTCCCTATAGTTCAATGGATAGAACAACAGCCTTCTAAGCTGTGGATCCAGGTTCGATCCCTGGTGGGGATAAGTTAGCTTGCCAGGTTGGGCTCTCGTGGTCTAGTGGTTTAGGACGCCACCCTCTCACGGTGGAAATCGAGGGTTCGAGTCCCTCCGGGAGCGATTCAATTTATTCTCATTGCACTTCGTATGGATCAAAAAACTTTTAAGGCGTCAGGAACAAATCAGAATTTTTGATGACCAAAGAAGGCTTTTTGAGGGCAGAAATCTGATAAGGGTGGTATGAATGCCTTTAAGGCAAAGATGATCTATGTGAACGATCAGATTAAGGCGCCGAGTGTGATGATCATAGATGATGAAAAAAATAATCTTTGAACTTTTCCAAGGAGGATTGCTCTGGAAATGGCTCAGGAAAAGGGGCTTGATTTGGTTCAATTGACTTATGATCCTGAGAAGATGCTCTCTACGGTGAGGATGACGGATTATGGAAAGTATATGTATCAAAAAGGAAAAGAAGAAAAAGAAAGAAGGAAGATTCAGAAGGGAAAAGATACAAAAGAATTGAAAGTGAGTTATTCTATAGGAGATAACGATCTTCAGCTCAAGATAAAGAAAGCACAGGAAATTCTTCAGGACGGAGATAATGTGAAATTTTCTATCAGGCTGAAGGGGCGTGAGAGAATGTATGCTGATAAAGCGGTTCAAAAACTGAAGCTTATTCAGGATTCGCTTGTGGAGTATGGAAAATCTCAGTATGATACTCCCAAAAAAGAAGTAAACGGATATAGTGTTATTTTATTTAGTAAATAGGCTTGATTCATGGCTAAGAAACTTAAATCGCATTCTGGAGCACTTAAAAGGTTTAAGATTACCAAGAAAGATAAAGTGTTGTCTAGTAAGCAGTGTAATAATCATCTTTTGACTAATAAAGGAAAGAATAATAAAAAATTCTGATATGGAAAAGAGGTTGTTGGTGCTGCTGCGAAGATGTTGAAAAATTTACTTCCTTATAAATAAGATAGACAATGGTAAGAGTAACGAATGGATTACAGAGGCAGAGAAGGCATAAGAAATTTATTGCTCAGGCTTCTGGGTTTAGATTGGGAAGAAAAAATCTCTACAAGCAGGTAAGGCTTGCTTTGGTAAAACAGGGTCAGCATGCTTATGTAAGTAGAAGGCTTAAGAAAAGGGATTTTAGAAGACTCTGGATTGAGAGGCTTTCTGCTGCGGTTAGAGAAAAAGGTGGAAAATATTCAGTATTTGTAAATACTATGTATCAAAAGGATATTAAACTTGATAGAAAGGTGCTTTCAAATATTGCGGTAGCATTTCCTGCCGTGTTTGATAAAATTTATGATGAGGTGGTAAAATAAGAAATGAGGCGGTAGCGATCGTCGCTATCGCTCTTCTCTCGTGTTTACAGAGCCGAGGTGGTGGAATTGGTAGACACGAAGGACTTAAAATCCTTTGAGGCTTATACCCTCGTGCCGGTTCGATTCCGGTCCTCGGCAACTTAACTTACAATAGGTTTGATTCAGGTTTTAATTATTATTTGTTATATTCATGTGATTTACCGTTCGAAGAACTGGAGATTTGAATACCAACGAAAGACTTATTTCTTTCTACAAGAAAGAGCTTTGGGCTTCTCATATTTTGGATAAGGCAAAGAAACAACAACACTATGGTACTAAATGGTATAGAAAGTTTGTAAGTTATGATTTTCCTTCTTCTAGAAGATATAAGAGAATGAGAGCTATCGTATCTGATGCTTATAGAAAGACAAAGAAGAAGTATAATACTTTGGCACAACTTGGAAAGATTGTGTAAGCTTTATTTTGATATTTTACTAGAGAATGTATGCAGTGGTAGATTTGCAAGGGCATCAATATATTGTTTCAGAAGGAGCTGAACTTATTGTAGATAATGTAGATAAGGCACAAGGTGAAAAGTTGCTTTCTGATAAAGTGCTTGCACTCTTTGATGAGAAGGGTACTGAAGTAAAGGTAGGAACGCCTTATATTTCTGGGGCTCAGGTTGAATTTGAGGTTGGAACTACTCAAAAGGGAGAAAAGGTTAGAATCTTAAAGTTTAAGAGAAAGACAAGATATGCGAGATTGAAAGGATTTAGACCTCTTCAAACGGTTTTAACTGTTAAAAACATTAGTGCTTAATGGCTAAAGCTTGAGTATTGGAACGAGATGGCGAAATATTGGAACAACTTCCTGCTGGGAAGTATATGGTTAAGCTTAAGGATACTGATATGGTAATTTCTTGCTATAAGGCAGGGAAGATGAAACAATCGCATATTGGAATTATTGAGGGAGATCGAGTGAAGGTTGAGGTTAATCAATATGATATGAAACAATGAAGGATTGTCTACAGATATAATGTGGCGACCAAAGCTCAAGCTCATGAAAATGAGTAGGGGCATCTTTAGATACTGTTGATGAAAGGAAAATGAAAGTAGTAACTTCTCTGAAGAAAAGATGTATGTACTGTCAGATTGTAAAGAGAAAATGAAGACTTTATGTGACATGTAAGAGAAATCCTAGACATAAAGCTAGGCAGGGGTAGTTTTTTATTTATTGTATTTTTTGAGAGATGTTTAGATTGATGGGACATGTTCTTCCAGAAGAAAAATCTATTTGGATTGCGTTGACGATGATTTATGGAATTGGAAGAGAAAGATCAAAGAAAATCTTGGGGAACCTTAACATTCCATATATGAAGAAAGTAAGTGAAATCTCAGAAGAAGAGCAAAAGTTAATCTCTGATGAATTGAAGAAGTATGTTTTGGAGAATGATCTCAAGAGGGAGGTTGCTTCTGCAATCAAAAGACTTAAGGAAATTAAGTGTTATAGAGGAATGAGGCATAACCTTGGACTTCCTGTAAGATGACAATTGACAAGAAAGAATTGAAGAACTGCAAAGAAACTTCTTGGAAGGTCAAAGGTAAGACCAGTATTGAAGAAATAATTTTATCTCATTTTTTTCTATTGAAATGGCTGTTACGAAGGCAAAGAAAAAAGATATTAAAGTGGTAAGTGGGGTATTGCATGTGCATACCACTCCTAATAATACTTTAGTGACTTTGATTGATCAAGAAGGAAATAAAGTTCTTGGTGGTGGAACAGGAAAGGTTGGCTACAAGGGCTCAAAAAAGAGTACTCCTTATGCGGCAGAAGTGCTTACTAAGCAAATTCTTAAAGATGCTCAGAATTATGGATTGAAGGAAATCTGAATCATTTTTAAAGGGATTGGTATGGCAAGAGAAGGAGTTTTTAAGGCGGTTACCGAGATTGGAGTAATTGATGTTGCTTATATTAAAGAAGAAACAGGAATTCAATTCGGTGGATGTAAAGGAGAAAGACCAAAGAGAAACTAATCCTTGGTAGACGGGTAAATATGATCATCTTTCAGTTTATTTATTATTTTCATAGGAGATATGAGATACACAGGAGCTAAATTTAGACTATGTAGGAGGGAAGGAGTAAATCTTTTCGGTTCTCAGAAGTATAATCTCAAGAAGAGAAGGGCTTTGCCTGGACAGCATGGAGCAGGAATGCAGAGAAATTCTGAATATGGGAAACTTTTGAGGAATAAGCAAGTAGTGAAGAGAAGTTATCTTCTTTCAGAGAAACAGTTTGCAAATATTGTGAAGAAAACTGCTGCTAAATATGCTAAAAACAATAACCTTTCTCATGATGTAGCGTTGTTTCAGTTTTTGGAGAGGAGACTTGATGTTATTATCTTGAGGTCAGGACTTGCAAATACAATCATGCAGGCGAGACAAATGGTGAGTCATGGTCACTTCCTTCTTAATGGAGTAAAACATAATGTTCCTTCCACATTTGTTGTTGCAGGAGATAAGATTACTCTTAAAGATAAGCTTAAGAATTCAGCGTTGTATTCTGATGCGTCAAAGGCTTCCCCTGCTAAAATCCCTTCTTGGATTAAAGTGGATAGAAATAGTTATGCTGTAGAGGTACTTGGAATGCCACAGACTGGAGAGATTGAGATACTTGGAGATCTCTTGAAGGTGATTGAATTCTATGCGAGGGCTTAATAATTGTTAAAACAACAAAAACTTTATATCATATTTCTATTTCTATGTTGAATATACAACACTTTATTGGAGTTCCGAAGATTGCGTTCAGTCAATTGGATGATGGTGCTACCAGGTTTGAATTGAAATATGTTCCTAGAGGATTTGGGCATACGCTTGGAAATGCTCTCAGGAGGATAATCCTCGCCTATAATCTTGGTGGGGCAGTTACAGGATTGAAGATAAAAGGAGTTCCTCATGAATACGATATTATTAGTGGGGTGAAGGAAAATGTGATTAGTATTATGTTAAACTTCAAGAAACTTCGCTTTAAAATCAATGAAAATGCTGATACCCTTCAGCGAATCTCTCAGAGATTTAAAGGGATCGGGAATTATAGTGCTGCGGATTTAAAGTTGCCTTCAGGAATTGAGCTCTTGAATGGTGAGGAACATTTGTTTGAGATTACTGATAGTTCGGTAGAACTTAATATGGAAGTGAGAATCGAGAAGGGATATGGTTATTATAGTTTGGATTATTTGAGACAAAGAGATGAGAAGGGGGATGGATCAGAAGTAAACTTGCTTTTGATTGATAATGATTTCTCTCTTGTAGATTATGTAAGGTATGATGTAGAAGAAGTGATTGAGGATTTTAGTGGAAGTACTAAGGATGCACTTAATCTTGAGATCAAGGCTAAGTATGTATCTATTGCTCCTCAGGAGATTGTAATGTTTGCGGGAGAGGTACTTGCAAGTTATGCTAAGCTCTTTGTGTTTGATAATGTATATATTGATAAGTCAGTTTTGGTTGATTATAATGATCTTAGTGAGGAGGCTGAAACTCCTATAGAAGAAGTTAGCTCTGTTAAGACGATGCCGATTGATGCACTTCCACTTAGTGAAAGGACAAGAAATGCATTGATCAAGAATAATATTCTCTATGTTGAGGATTTAGAAAGAAAGAAGAAATCAGAATTACTTGTGATGAAAGGAGTGTGAAGAAAGGCAATTGATGAAATTACTTCTTCACTTACAAATATTGGTAAGGCATTGCTCGGATAGAAAGGAAAAATTTATCATTTATTAGTGTAGATATAAGATGAGACATCAGCAGAATAAGCTCTTGCAATTGAACACTGGAGCAAAAAGTAAAACTGTTTTTATGAGAATGCAACTTTCTAGTTTGATTAGAGCTGGGAAATTGACTACGACTCCAAAGAGAGCTAAGGTTCTTAAAGCTTTTACTGATAGCTTCTTCTCTACTTTGTTAAGTTTTGATGCTAAGTATGATGAAAAGGGGGCTTTCAGAGAGGCAATTAGATATGTAAAATCTGTTGTTTATGGTGAAGCTGAAGGAAAAAAGGTAATTGAAACTTTGCTTCCAAAGTATAGAGAGGAGAAGAGAACTTCAGGTTTTACTACTTCTTATAAGCTTGGATTTAGAGTGGGGGATGCTGCTGCGAAAACCATGCTCAAGCTCGCATAGAGAAGAATCAATTTATTTTAATTATCGTATAGGACTAATCATGGAGTTTACGAAGAAAGATTTAAATAAAACCATCTGGATTAGGCAGACTGATCAAGAGAAAAATAGGAGATGGTATCGTGTAGATGCTACAGGAAAGACCCTTGGAAGATTGGCTGTGGATATTGCTAAGAAGCTTATGGGAAAGGATAGAGCTTATTATTCTGATTTTCGAGATGCAGGAAGTTTTGTTATCGTAGAAAATGTTGATCAGATTGCAGTGACAGGGAAAAAGCTTACTGATAAGGTTTATTATTCTTATAGCGGATATAAGGGGAATTTGAAGAGTATTACTCTTGGAGAGCTTCTCAAGAAAAATCCTGGTAAAGCTTTGTGGTATGCGGTAAGAGGTATGCTTCCAAAAAACAAGCTTAGAGATCCTAGAATTAAGAGATTAAAGCTTATTAAAGGAACGACTACGAAATACGATAATTTTAAACCAATTAACCTTTACAAGTAATGACAAACAGAGAATACACTAATGCAGTTGGAAGAAGAAAGGAAACGACAGCAACTGTAAAACTCTATGCTAAGGGAGCAGGAAATATCCTTGTAAAGACAGTAACAGGGAAAGAATATACGCTTGCTGATTACTTTGGAGGAAATGTTTACCTCTATAAGAATGCAGTATTACCTTTTACTACGCTTGGTAATGATGTGCTCAAAAAATTTGATGCAGAAATTAAGATTGTGGGAGGGGGTATCGCTGGGCAGTCTGATGCAATCAAACTTGCTTTTTCAAGAGCTTTGGTAGAGTGGAATTCAGACCTCAGACCTACTTTGAAGCCTCATGGCCTTCTTAAAAGGGATCCTAGAATTAAGGAAAGAAAAAAGCCTGGTTTGAAAAAGGCGAGAAAGGCACCTACCTGGTCAAAGAGATAATTTTTGTACGGATGAAAACCTGATTTTAAATGTCAGGTTTTTTTGTATTAAACTATATTCAGTTTGTTGTTTTTTTGGGTATAAACTCCAATTTCTCTTATTTATTAGTGGTGATTTTTGTGTTATAAGTTTTCTTTCTGAATCTCGGCTTGATTTTTATGCTGGGAAAAGTATAAGTAAACGAATTTTTTGTTCGCGTTTTTTCGTGCCTTAAAATTTGGCTTCAGCTCCTGTAGTTCAATGGATAGAATAGGCCCGTCCTAAGGGTTAGATGCTGGTTCGATTCCGGCCAGGAGTAGATGACATTCCTTGGTGGTAAGGATTCCTTGTTTGAGGAGCTTTACTGTGGGGTTTGATCAGCTTTCATTTTATTTTTTTAAATCCATTATCCAAATGGCAAAAACAACACAGGAAAATGTATTTCAAAGACACGATAAAGATACCGGGTCTCCAGAAATGCAAATCGGGATTTTATCTGATGAAATCGCAAAGCTTCAGGCGCATGTGGCTGCAAATGCAAAGGACTATGATGCTAAGAGAAGTCTTTTGAAGAAGGTAGCAAAGAGAAGAAGCTTTTTGAGATATCTCAAAGGGAAAGATCTTGATGCCTATACAAAAATCTCAAAAAAAATCGGACTAAAAGTGTAGTTCAATTAACCGTGAACAGGTAAAAATAAACAATGAAAATACTTTTTTGATGCTGATCTTCTAAATAAAAAAGGAAAAATCTGACTCAAATTGTTCATTGTTAATTATCAACTGTTCATTGTATTCTTTTATTTCTTTATTTTTATATTCTAATGGCTAAAAAAGCTATTGCCCCAGCGGCAAGCCAATTTACCGTTCCTGTAATCAAGGAAGGGCAAAAGGTAAAAGGAACCATCCTTAAAGAAATCGAGAACGGAGTTCTCGTAAACTGTGAAAATGGTGCATTTACTGGAGTGATTCTTTCCAAAGAAGCGAAAGAACTCAAAAGATCTCAGTTTGATCTTAATCCATCTACTGAACTTGAGCTTGAAATCATCAATCCTGCAGTAAGACATGAAGATGGATACTATATTGTATCAGTTACAAGACTTCTCCAATACGATGTATGGAAGTCAGTGATCGCAAAGTTTGAAAAAGATGAAATTATTACTGTGGTTCCTACCGAGGCAAATCTTGGAGGACTTTTGATCGATATGCACGGTATTAAGGGATTCATCCCATTATCTCAATTGGCTCCTATTCACTATCCTAGAGTAGAAGATGGAGATCAAGATGCAATCTTTGATAAACTTCTTGAACTTATCGGACAGGAAATCAAAGTAAGAGTGATCAATATTGATGAAGAGGACAGAAGAATCATCCTTTCTGAAAGAGAGGCGCTCAAAGAAGAAAGAGAAAAGGTGCTTGCTGAACTTGAAGTTGGAAAGATCTTTGATGGTGTGGTTTCTGGAGTTTCTTCTTACGGACTTTTCGTGACTATCGGAGGAACTGTTGAAGGACTTGTGCATATTTCAGAAATTACTTACGGACACGTGAACGATATTGATAAACTCGGACACATTGGAGACAAGATGCAAGTTAAAGTGATCGGTCTTGAAAATGGAAAGATCTCACTCTCTAGTAAACAGCTCAAGGGAGATCCATGGGAGGAACTTCCAAAGAGATACCAGGTTGGAGATATTATTGAAGGAGAAATCGTGAGATATGTTCCTTATGGAGTATTCGTGAGAGTATATGAAGATATCAACGGACTCGTGCATTTGAGCGAGCTTTCTCAGAAATCAGTGAATAATCCAAACGAAATCGTGAAGCTTGGACAGATCGTGAAAGCAAAATTGATCCTCATTGATCCAAAAAATAGAAAAATCTGACTTTCTATGAAGGATTTGGAACCAAAATCAGCTGATACCGAAGACAAGCCAGCGAAAAGAACTCCAAAAACAAAAGGAAGTCTTGAAGACGTAGTAAAGAAGATTGAAGAAAGTGAAGAATAGTATTTATGAAAAATCCTGCAGAGTGATTGATATCGTGCAGGATTTTTTGAATTGAAAGAATGTATATTTACTTTTTAGTTGGTGTATTGGATCGGACTTCTTTCTTCTCGTCTTTTTTTTCAGAGGTTTAGGTTTATCTCATCCTGAGCATAGAGTCTACAAACTACCTCGCCTTTTTTGACTTTATCTCAGGTTTTTTTGTAGAGGTAGAGTCCAGCTTGATCTACTACGGGGCAGCCAAGCTTTCTACAAACTGCGTTAATGTCATGAAGATCAATCATTTTGATTACTCAATCCTTCTCTGCGATAATATCGAACGTATGAGATCCTAAAGGTAGGGTTTCAGAATCTACTTTTGGATTTCCTCCTTGTGCTTTGATAATTTCTTGCATTTTTTTCCATGCAGCTCAAGAAATAAGCTGGTTGGTTGCTATTTTTTTTGCTTCTTTCCCTTTTGCTAGTCCGACCTCTTCAATGAGTTTGGTAGCTAGGAAGATGACCTTATTCTCAAGATCCATAGCTCTGTCTGGATGTTGCTGCAAAATTCTGAGCACTTCACGCACTTGCATCACTGCTCCTACTCCGTTTCCAATAACTTCATTGGCTTCCGTAATCTGAACAGACATTTTCATTCAGAGTTTTTTTCCTACATATTCAAACTTTTTTTCCAGTCTTTTGCTTCTTTCATAGAGCTTACCTTAGCAGTTGGACCAACAGGAATATCAATCAAAGAATGGGTCACTCCCATAGCATATTTCTTCGCCATAATTGAACTCACGACCTTAGCTCTAGATTGCATAGAAAGCGGATATTGCACCTTGATCAATTTGTCATCAGCAGGAGCAAGATCAAGTCCTCCTCATCGAACAAGGCAACAATTCTGATCTTTTACCAGATTTTCTATCCCTTCTTTGTTGAAGTTGATATTCATGAGCACATTTACACATTCCCCGGTTGCTGCTGGAGAAGTGATGGATTTGGAGAAGTTTTTTGGAATTTTGATACCGAGGCTTGCGATGAGGGGAATCAGAATCATTGTCGTTTCATTTCCTGGGACTCCACCGATACAGTGCTTATCTGCGATAATTTCCCCTTTGGGATATTTGAACATTACCCCACATTCTGCCATCGCTTTTGCGGTCTGATACATCTCTTCATCCGTCGTTGGATAGAAAAAACTTGAAGCGACATAGTAAGTCATCATTGTGTCATCAAGCTTATTCTCTGAAATATCTTTGATAATTGCAAACATCTCATCATAACTAATGTTTTCTCCTCTCATTTTTTTTCTGATTGCTTCGTAGGAAGCGTTCGAACTTTCTGCGAGAGAAACTGCGATGAGTTCATCATTTTTTAGGGAGATTTTATCAATAATGTCTGCATAGATTGCAACTTCCCCTGTTGGGATTGCCTCTGAAAGAGAAACGTCAGCTACGATATGTTCTCCGTTTGTTTTGGTGAGCTGGACTTTATTATTGAGATTGATCCCAAATGCCATTGCTTGTTTTGGATTGAGGAGGACAATCAGTCTTTCTCCATCTTTAATATCTAGAAGCTGAACTTTTGCTGGATAATAGGATTCTGAGGTTTCTTTAAAAACTAAAAAGTCTTTTTTTGTAGTTTTGGATTTATGAGATTTGAACATTCTATCTTTGAAGAAAAATAAAAAAATGTCATCCTAAGTATATGAAGAAATGAAAATAAAGCAAATTTTGATAAGAGGAAGTTTGTTTTCGGGTTTTTGTTTTATTCTGTTCAAGTGTCGGGGAATGGTTGTTATGTTTTATGTTTGAATGGCTTGTGTTTTTTTATTGGATCTTTCCTATGATCTGGAAATATTGTTGATTTTGTAGACAGGAATGCTTATACTTTCTTTGTTCTTTTATTTTTTTAGGTTTAATGTTCTCAGAGGCTGATACGAGATCTAAATTCATCGACCCCAAACTTTATGCTCAAGGTTGGAAAGAGGAATATATCGTTAGAGAACGGTCTTTTACTGATGGGCGTAAACTCCCCGGAGGAAAAAGATCAGCTCCACTTTTTCTTGATTATCTTCTCAAGATGTATGGACTAAATCTCGCGATTATTGAGGCGAAGAAATATGGTTCCCTTGTGACAGAAGGACTTGAGCAAGTTAAAAATTATGGAAAGAAACTCGGGGTGAGGTGGGTCTATACTTCCAATGGAGAAGAGATTTATGAACGAGATATGCAGGAGGGGGATGGAAAACTAATCTCATCTTTTCCAAGCCCTGAGGAACTCTATGAAAGATATACGGATGAGCATGCTGTTCTTAGGCAACAGCTTTTATCTGTTCCTTATTTTCTTGATAGTGATAAAAAGCCTCGTTATTATCAGGATTTAGCGATTACTAGAGCATTGTCGGCTTTAGCAGAGGGTAAGGAAAGAATCTTACTTACGCTTGCAACGGGGACTGGAAAAACCTTTATTGCATTTCAGATTGTTTACAAGCTTCTCCAAGCAAAATGGTCAAAAAGCTGATTTAACCGTCGTCCTCGTGTACTGTTTTTGGCGGATAGAAATATTTTGATCGATCAGGCACTTAATACTTTTAATCCTATAGAAAAAGACTGCAAGAGAATCAAAGGATCAGAAATAAAAAAAGCGCTTGGGAAAGTGCCCACTAGTGCAAATGTGTTTTTCTCGATTTATCAAGCTTTGATTTCGGGGACGGATGAGGAGACTGAAATTATAGAAGAAACTTTAGTTGGTAGTGAGGCTTTGGAGAGTGGATATTACCTCCAGTATGATCCAGATTTTTTTGATCTTATTATTATTGATGAATGTCATAGGGGAGGAACAAAGAATGATGGGAACTGGGCAGAAATCCTTAAATATTTTTCATCAGCAGTGCATCTTGGGATGACAGCCACACCAAAGAATCAGGATAATATCAATACTTATGCTTATTTTGGTGAGCCAGTTTATCAATATTCACTCAAAGAAGGAATAGAAGACGGATTTCTTACTCCATATAAAGTCAAAAGAATCCGGACCAATCTTGATGAATTGGTAATAGATGATAAAGTCGAGATTCTCGGTGGAAATAAAGAAAAAGATTTGTATGAGCTCAAAGATTTTGATAGAAAAATTATCGTTCCTGAGAGGATAGATCTCATTGCAAGAACAATCCTAGAACATATCAAGCCTCATGAAAAAACGATCATTTTCTGTGTGGATCAGGAGCATGCTGCACTGATGAGAGATGCGATCAATAAACATAAAACCGTGCGTGATGGAAATTACTGCGTGAGAGTGACGAGTAATGAAGGAGAAATCTGAAGAAAATATCTTGAGCTTTTTCAAGATAATGGGAAAACGATTCCTACTATTTTGACGAGTTCGCAGATGCTCACAACAGGAGTAGATGCCAAGAATGTCAGAAATATCGTTCTGCTCCGCAGTATTGGAAGTATGGTCGAGTTTAAGCAGATTATTGGTCGTGGGACAAGAGTCTTTGAGGGGAAGGATTATTTTACAATTATTGATTTTACTGGAGCAACGAATCTTTTTTATGATGAGAAGCGAGATGGTCCGATCTCTACAGTGTCTCCAGAAGACCAAGCAAAAGAGTCAAAAAAAGAAAAACATCAGACTTTTGATCCTGTTTCTTCTGTTGATTCTGAGGAGCCTCATATTCCAAAGCAGAAACTCAAAGTCAAACTCCTGGATAATAGAGAGCTCAAAATCATCAATATTGAAACCAGATATATCGGAGAGGATGGGAAACCTTTAACAGCAGAACAATATCTCTTGACTCTTGTTGGTAAGCTTCCAGCACTGTATCAGTCAGAAGAGCAGCTGAGAACCCTTCGATCTGATCCCAAAACAAGAGAAGATTTGCTCCTAGAGCTTAGGAAAATTGGACTGGATGAAGAGCATTTTAGTTTACTTCAGGCGATGTTTGAAGCTCCAGAGAGTGATCTTTTTGATGTCTTGACCTATCTAAGTTATGGTGAGGAAATGAAGACAAGAACTGAAAGAGTTGGTCGAGTGTTAGAGCAATCCTTGATGAAAAAAATTGAAAATCTGAGTGCAAAAGCTTTTTTAGAGTATCTCTTAGCATATTATCAGGAGCATGGAAGTACGGAACTCGTGCAGTCCAAGATGGGAGAACTGATAAAGCTTTATGGTCGTGGTCAGATCAATATTGTGGATTTTACAAAGGCCTTTGGTGGGCAAGAGGTATTAATGCAGGTATGGAAGGACTTGCAGAGGGAGTTATTTAGGATTTAGATTGATAATGTTATACAATGGCTAATGAAATGGGTAAACAATATTTTCAATATGTTATGGGGCTTATAAAAGCTCTAAGTTTTTTTAATCAATATAGAGATGGTATTATGTATTATGATAATAAACAATTGTTTTTATCTGATATCTTTTGAATAAAAGACTGGTATTGAAAATGAGAAATTTTAGATGATACAATACTTATTTCAAAAAACAGTCAGGTTATTTATTTTTGCCAAACAAAAGGAATAGAACAAAAAAAGCACTTCACGGATTCCAAGATAAAAAGACTTTTTGAGCAAGGTATAACTGAAATTATAGAATTATCAAAAATTATTCAAGATTTATCAAAATATTCTATTTTCTTTTTTATTATGACAAATGAGTCTAAAACATTTAACTTTTGAACAACATTATGACAGTATTCATTAGAAGAAGATAATGGTTCTTTTTTTACAATGTATAATGCTATCAAAATTGAATTAAAGTATGACCATGATTTTGCTTGACCGAAGAGAAAAGATGATAATGATATTATAATAAAAAGTCATTTAAAAAATTTATATAAAGAATCATTTTGAACTTCGGCCATAGAGATTCTTTTTGCGCATATTTTTAAAAATAACTCTCATAAAAGTTGGTGAGTTAGCGAGATATATGATACTATATCACCATACAAACTAAAAGAGGAAGCAAATCAGTTTATTTTAAAGATGTTGGCATATGAAATAAGAAATCCCAATATGTCATCAAAGATCACTCAGTGGTTCTATGAGCAATTACACAAAAATAGAAAGGATTCAGCCTCAATTTTACAAGATCCTTTAAAGGTAACTACTCTTCTATGAGAGATAAAACAGGAAGACCCATATTCCACATATGAGCAAATTCGTAAAATATATTCTGGAGATAATGGTGCAGATAGTTATAAGATCATGGTAGATTTATTGAATTCGTGTGATATTAATTTTCAAATAGCCGATTTGGAAAAATATATCAAAGAGAACGATCTCGTATGAAAACACCTTGCATTATTTAAAGATCAAAATAAAATAGAAAAAATAAAAAATATGATGATATAGTTTATTTTATATTATAAGAATATGAAAAATACAGAACAAAAATTGTTAGAATATGTAGAGGGATTTATTGATAAGAACAAACTTTCTTTGGAAGAAAGAACCTACTATATAAATCTTTTAGTAGAATGAAATAGTAGTATATATGATAAAGAATACATGAAAGTTTATTTTTCTTATTTGGTAGATAGATTGCTTTTAGAATGAAAAATTACACTTGATATTTAGTTTTTTAATTTATCCAATATGAACCCTACTTATTTTATTAAAAGCATTAGTATATTTGGATATTTAGAAGGTAAGTTATTACTAAATTCTAAGACAACTTTTTCTACTAATTTAGTTAATGCGATAATTTGAGATAGTAATACATGAAAAAGTTTGTTTTTATTGTTTTTATTCATAGGATCCTTTCCTTTTTCTTCTATAAGTTTGAATAATTTTATCAAAACATATTTTACACTTACTAAAGAAAAAGAATCCAATTTTGATAATGTAAGAGTTGAAGTTAGATTTCTTCTAAATTGAGAAGAAAGTTTATTAATAACAGAAATATCGGTTGAATGAGTAAAATATATTTGGAATAATTGTGAATATGGGAAAAAAGTATGAGAAAACTTGTCAAAAACAGAGATTCTAATGGATGAGTTTTCAAATTTATTTCATTTTTACGATAGTCTATTTAGTTTGGCTACTCTACCTAAAGAAATAAAAGCAAAGAATATTGTAAAACTTCAATTGAAAGATTTGTTTTTTTCTTTTCGATTTTTAAATAAATATAATTACGGGAATATTTTTTTACCAAGAAATATATATGGGAATATATCAATTACAGCAACACATAGAACTTTTTTCCCTATATATTTATATTTACTATTGCCGTGAGTTAGTTATGTACTCAGTAATTTATATGGAAATCTCGAAAAAAAGGAGAAAGTAAAAACATTAGATAAAAGTAAAGATAATCTTAATCGAATTATATCTAATTATCTGAGCAAGAAAAGGGAAGAAAAAGAAATAAAAAAAAGACAAGAAGCTTCCTTGTTTACAGAGGAAGAAATTTATAAAAATGAAGTTAAAAGTATTGTTCAAATACAGATTGAAGTTGATGCAATTATTGATAAGCTATCTATATTGGAAATTAATCTTTTAAAAATTGAGCAATATTTAGCATTTACTGGTTCTAAAATTAATTTGACGGTCTATCAAAAAGGATATTGGCTTGATAGGAAAAATGAATTAAAAAGCCTTATTGATAGGTATAGTAATGAATTAAGAGAGAAAAAAAATAGTATAGAAAAATTAAAAAGAGACAATAATAAATCTTTGTTAAAAACTGGATTCTATTCTGGTGTTTTGGATTTTGTTGACAAGGAGGTTGTAACAGAATGATTAGTGAGTTCTTTGTGGGAAGAGATTGGCATAAATATTGATTGTTGAATTACTTACATTGAATGAGAAATTAATCAAGATGTCGCTAAATTATGAGCATTGTTGTCTCAGGATAATCCAGATTTTCAAAAATTTAAAGATTTTTATAATGATGAAATAAAAAATGTAGGATTGGGTGTATTAGAAAAAGAATTGCAGGCTGAAAAATTTAGTATAGAAAAAGATTTGATAGGTCCAAAACTTAACGTGGATAATGGATTAAATATATTTGTGAATCTTATAATGATCAAATATTTGTATCTTTGTCTTAAAAAGAATACAAGATTTTTCCTTCCTCCACTTGTCATTGATAATACATTTGCTGATTTAAATTCATGAGATGGGATATATAAAAAAGCTATATTTAATTATTTAAAATGATTCATACAGGAAGGAATACAAGTTATTATAACAATATGAGATGATTATAAAGATTTGAAAAGTGAGATAGAATGATGTATTTTAAAAATCTCTCCATTGTTTGAACAATAATGCGGTTTTACTCGTTTACGTTGAATATGTACTTAATAGTAAAAGATATTAGTTATTTTATTCGTTGAGTCGTTTGCTATTTTACAATAGAGTAATATCCTATTTTTGCTAATCAGGCAATTCAATGGGTTTTGCTACAAATTCTTTCCATTTATATTATTCTTAGAATAATTTGTTATGCTATTACGCGAATTTTTGCATCTAAATTACAGGCAAGAACATCAACAGAAAAGTCAATTATTTATTTTTTAATATATTTGATAGTTGCTATTATAGTCTATCGAATATTACAAAAATTAACCCAATTACAGATATTGCCTCTTTAAGCTATTAAATATTTTACATATTATATAATGATAAATAAAGAATTAGAGGAACAATTTGATATCCAAATTCAACTCATTCAATTATCAATAAAAGATTTTGATAAAGGAGATTTTCTTGCGGCTATGAACTTGGCAATAAGAATTAGGTTTTTAATACATGATACTAATAGGTCAGTTTCATTATTAACACAAATGTGATATAAAGAAAAATTGTCATATTATGATACTTCAGTAGAATGTATTGAAAATAAAGGATTTATGCCATGACCATATGTTTGATTGATGGAGTTTGTGATTTGAAATGATAAAGCTTTTGCGCTTCTGGATCATGCTCCTGATTGTAAAATAGTATCGTTTAATGAGTGGCGGAATGGAAAAGTCTTCATTGATACTGATGGAGCTTCTCTGACTCGTAAAGATGTAGTTTTTAATATTGCAAATAAAATTTGAGCACATGTTGATTTAAATTTTGATGCATGATATGAAAAAATAATAAGAAACCATTTATTATGAATTGCTGCTTGAGATCGGAAAGGTTGATATAGGCCTATCCAAAAACTAGAATATATGGCAATTAGACAAATTACACATGAACTATTGAAATCAATATTTGAAAACTATAAATGTTGTTATAAATTTGAATGAAGTCGTTTTATATGATGTGTCTTAACTTTTAATATATAATATTCAATAATACATGCTTTCATCCAACCGACAACTTAAAAAACTTTGAGAAATATGTAAGTTCCAAAATTGATTCACATTTAAAAGTGAATTATTCAGAGAGAAGTGAGAACCTATTCTCAGAATTTCAAACATTCAAAATAACTGATTTTCTGATGATAAATTGGTTTTTTTTGATGTTAATGATTATGATAATTTAGATAATTATATAGTTTATCCTTGAGAGCTTGTAATTGCAATGTCTTGAGCAACTACATGAAAATTAGCAATTAATAATAAAAACAAAAACTATTATTTAAATCAAAGAGTTGGTAAGTTTATATTTAAAGAAGATGTTACAAAAAAATATTGTCATTATTTTTTATGAACAAAAATTGAAGAAAACTTACACAAATCTTCATGATCAGCAATCCCAAACTTGAGTACAAAACAAATTCTTGAAATCCAAATCCCCTTTCCCCCACTTGAAGAGCAAAAGAAAATCGTCGCCTATCTGGATGAGCTCAATGCAACGATCTCCAAGCTCAAGAGTGAATATCGGTCTCAACTTGCGATGCTTGATGAGATGCGAAATTCTAGTCTTGACCTAGCTTTCTGAGGATCTAGAGAGAGAGAGAGAGAGAGAGAGCATACAACTTAAATAATTGGAAATGGGTAAAGCTTGGCGAAATTGCAAAAACTCAGAGTGGTGGAACTCCTAGCAGGTCAAATCCATTATATCGATGAGAGGGGATTGTTCGATTGAAATCATGAGAGTTGGAAGATAATGATTGTATTACAAAATACACAGAAACAATTACTTTATTGGGGCTTGAAAATTCTTCTGCAAAAAAGTTTACTAAAGGAACTTTATTACTTGCTATGTACGGAGCAACTGCGTGAAAGTTATGAATTCTATGAATAGATGCGGCAACAAATCAAGCTGTTTGTGCTATTCAAAATACTCAAAATAAGTTCGTCGAAAAGTATATTTACTATTTTTTGTTAAAAGAGAGGGATAAGATTATTAGAGATAGTTTTTGATGAGCTCAACCAAATATTAGTAAGACTTATATAGATAATATTGAAATTCCTCTACCTGACTTAGAAACGCAATCCCAAATCGTATCCTATCTTGATCAAGTCCATCAACAGATCACTATGCTCAAAACTCAAGTAAATAGCCAAATCGAGCACTGCGATGAGCTCTGGCAGAGTAGTTTAGAAAAAGTCCTCACTCAAGGAATGGAAGAGGATCTGAAGTCAGATTTTTCTTTTCTTTCTTCTTGCAAAAGTTAGAGTTATCGTTATAAAGTATTCGTTTTCAAATACTTTCTGTACACAAAATGAAGCAACTAACAAAACTTATGATGACCAAGCAAACGGTCTTTAATATAGAGGAGCTTACTAGCTATCTTCAAATCCCTACATCAGCAGGCTTGAAAAGTTTTTTGCAAAGAGCAAAACAGTCAAAAGCTTTGCTTAATCCGATCAAAGGAATTTGGACTTTCCCGATATATGATCCTTTTGAGTTCTCATGCAAACTCAATTCTGATGCTTATATTTCTTGTGAAACGGTACTTTTTAGAGAAGGTATTTTCTTTCAGTTTTATGGGGATACTGTCAGCTCAGTTACCTTCAAATCAAGGAGTTATGATATTGATGGTCAGCATTTTATCTATTATCGTATTCAGCCAAAGATTGCTCAAAATCTGATCGGAATAAGAGATTATGATTGTTATCGTATGGCAACTCCTGAGAGAGCTTTGTGTGATTATATCTATCTTCATCCCAAGGCGAGTATTGATGCGCCAGAGTTGATCAATCAGAGAAGAATAGAAAAACTCTATCCATTCTATCCTAAAAAAACGGTTTTAAGAATTAAACAGCTTCTCAATGCTCAATTATAATAAGCACAAAGAATTTATGTTCAAAATTCTTATTCGTATTTTTCAAAGTCCGCTTAAGGATGTACTTGCTTTCAAGGGAGGAACGCTGGCTTTCCTTTGCTATGGTTTACCGAGATTCTCTACAGATATTGATTTAGATTTACTAGATTATAGTAAGGAGCCAGATCTATTAGAGATAATGCAGGATATTTTGGAAGATATTGGAGAAATTAAGAATATGACTTTAGGAAAGGAGCTTCATCGTTGGATTTTTCGCTATGATCCATCGACTATGAATATCAAAGTAGAGGTCAATAAAAGACCGCTTGATGGCAATATTTATGAGCGAAAAACCATTGAGAATACTCAAGTATATTGTATGAGTCAGTCTTCTATGGTAGCAAATAAGATAGTTGCTCTTTATGAGAGAGCATATCCTAGAGATCTCTTTGATGTATATTATTTCCTTGCTCAGGATTTCCCTTGTGATAATGCAGTGATTGAGCTTAGGTCAGGGAAGTCTCTACACGAGCTTATCTGAGAGCTTCAAGAGATTATTCCAAGGTATTTCCTTCCTAATACCGTACTTATAGGGCTAGGAGAACTGCTCACTGATACTCAAAAACTCCGAGTAAAAAAGCATCTTGTCACTGAAACCGTAGCACTTCTCGGTCAATATTTTTTAAAAAATAAATAAAAATCAGATTTTATTTTTTGTTTAATCTTAGTATGCAAGCACAAATCAATCGTATTACTGATATTCTTCGCCGTGATGATGGAATCTCTGGGGCAATGCATTATACAGAGCAGATCAGCTGGATTCTTTTTCTCAAGTTTCTTGATGACTATGAATCAGCTCATGTATTAGAAAGTATGCTCAATGGTAAGGAATATACCAGATTACTTGCACCCGCCTATCAGCGATCCTCTCGAGCGGCTCCTAAAAAAGATGGAAAGTTAGATCATACGAAGGCTCTTGTTTGAGATGATCTAAAGGAGTTTGTCAACACCCAGCTTTTCCCTTATCTCAAGTCTTTTCGTAATGGAGAGCGTAACTATCATAGTATGAAGTATAAGATTGGGGAGATGTTCTACTTTCTAGAAAATAGAATAGAAAACTGAAATACGATCAGGGAGGTTATTGATATTATTGATGAAATGAATTTTCAGTCGCAGTCAGATTTATTTGACCTTTCTAAAATTTATGAAGACCTTTTGCAAGGGATGGGGAATGATGGAGGAAATAGTGGGGAATACTATACTCCAAGAGCAGTGATTAAAGCCATGGTAGAGTGTCTTGATCCAAAGATTTGAGAAACAATCTATGATGGTGCCACAGGTTCTTGTGGTTTTCTGATAGAAGCGTATCAATACCTCAGACCTCAAGCGAGTACTACTGAGCAGCTTACTTGGCTCAATCATGAAGCTTTATGGGGAAATGAAAAGACACCGATTGCTTATATTATGGGAGTAATGAATATGATTCTCCATGGTATCGAAAATCCTAATATTAACAAGAAAAATACGCTTGTAGACAATATTAGGGATTTTGAGGAAAAGGACGCTTATGATATTATCCTTGCAAATCCTCCCTTTTGAGGAAAAGAAAAAGATGCTATTCAGTCTAATTTCCCTATCAAGACCAATGCGACAGAGATGCTTTTCCTCCAACATTTTATGAAAAGATTAAAAACCTGAGGAAGATCTGCGATCATTGTCCCAGAGTGAGTTTTGTTCAATACTTCCAATGCTTTTCAGGAAGTCAAAAAGCAGTTGCTTTCAGACTTTAATTTGCATTCTATAGTTTCTTTGCCTGCGGGGATTTTCTTACCGTATAGTGGAGTTAAGACCAACATTATCTTCTTTGACAAGACTAAAGCTACCGAGCAGATTCGATATTATGAGATTGACCTTGGTCGTAAATTGACTAAGAATAAGCCAATTACTTTTGAGGAGCTTCAAGGTATGATTTTAGCTTTCAAAAAAAGAGAAGTCTGACCTCATTCTTGGGTTGTGACATCTTCAGAGATCAAAGACTATGATCTTTCTGCAAAAAATCCTGCAAATATCAAAGAAATTATCCATAGAGCTCCAAATGAGATTATGGAAGATATTATTAGGACAAATGAAATAATTTTTGAGGGAACTAGTCAGCTTCGCACTTTGATTAAATAAGATTCTTTTCTCTATAATTTGATGTTCTGTGAATTAAAATCTGACGTTTTTATTGTTTTAATGTAATGTTTATTCTGTTTCTGAGATGATTCTTTTATAGGATATATTTTTTGCTAGCTATTTTTCTTTTTTAAATCAATCTTAAAGAGATTTCTTGTTGCTTTTTTGCTTTTTATAAAGTTTTGTCTATACTTGAAACGCTTGGTTTTTTAAAACCTTATTTGATTGTATGAAGAAAAAAAGCACGACCTTATTACAAATAGTAGGAAATTGGAAAAAAAAAGCAGCATTACCGATCAATATTAGATGGGTTTCTATCTCGCTTTTTTTGTTTATGATGGGACGGTGATTAGGATGAGATACCTTTTTCTCTGTTTATGTAAAAAGTATTCTTGGATCAGGATTTGGGCTGATGTTTATTGGAACTTTATTGGCTGTCATAAAATTGTTTCTTGTGATTCCTGTTGGTGTGATGAATGATCAATGAAATGCAAGATATCTTTTGTTGGCTGGGAAATTGGTATATATTTTTTCTGGGCTTTTTTACTTTTTGGCAGGAATGCATAGTTCTCCTTTGCTTCTTGTGCTAGCTGTTGTTTTGAATGGAATTGCGAGTTCTATGATGTTTACGACTTATCGTGTGCTTTATGGGAAGGGAACTCAGCATAGTAACCGCAGTAAGATTTTCGGTTTTTATTTCTCCAGTATTAATCTTGCATATGTAATCTGAGCTTTGCTTAGTGCTTTTCTTGTTTCCTATCTTGAACTTCCTTATATGTATCTTTTTATTATTATTTTTGCGTTGTTGTCTCTGCTTCAGGATGGAAAGATTCAGGATTTTATTAAGAAAAAATTTTCTAAATCTTGGAAAAAGTGGAACTTTCTTGTAAATTCTGTAGATACTGATGTTGACGTAGACTTGTGTAATATTAGGAAACTTTTTGGGAAAAGAGGGGTAATAGTTCGCTTTTCGAAGGAGGCAGTTTCTTTTGCTTCGTGGAAAAGGATGTTTTCTACTCTGAAATCGTATGGTCGATCAATGTATACTGCACTTTCTGCTCAGGCTTTGGTAAGTTTTATGAATTATGTGGGATTTTTGTTTATTCCGATTATTGCGAATGATAATAATCTTTCTCTTTCTGAAATTGCTATTTTGTTCGCTGTGATGAGGCTTCCTTACGTGATTAACGTTTTGATCGGAGGATTTTGAGATAAATATAGTAAAAAAATTCTGATTACTATTTTAGTTTTTATTGGTGCACTTTTGTATATTTTGCTCTGATTGTATCATAATTTTTGGGCAATTATTGTTTTGAGCTTCTGAATTTCTCTGGTGATTGCGATGCTTTCTCCGGTTACTTCTGCATTGGTAATTGGATATGCTAGACCAAAAGATAGGTGACTGATGTCTGGATTGCAGGAGTTTGCTAGTAGGATTGGAGAAATTATCGGCTCTTTGGTTTTTGGAATGATCGTTGCAAAAATTGGAATGGATGATTCTTTTATTGTTTTGGGAGGGGCTCTTGCGGTGTTAAGTATGTATCTTTTGGTAAAGAAGCTCATGAGAATAAAGACGAAAGATGGTGAAGCGAAAAAAGAGGTTGAAGCTCAGCAGCCTTTACCCTTTTTGCAGAATACTTAATTGAGAAAAAATCCCTTTTCTCCATAATTTTGTTTGACTTTTTGGCACATTTCTATACACTTCCTCTTGTGGTCTAATTCTGGCTTTGGTTTTTTTGAAATTGATAAAATGGGGAAAGTAGCTCTTCTAAAAAAAAATATTGGGATCTTGTTGCTCTCTCCCTTTGTTGCTTTTGGAATTTTTCGAGTTTCTCAGCAAGATTGGAGTAGGCTAACGGCGAGCGTTCTTGATATTTCAGAAATTGAAACGATTGAGAAACAAGGGCGAGATGTTGCATACAAAGTTCATGATCAGCTTTTTGAACTTTTTGGTTCAAAGCAGCTTCAATGAGGTGATGCTTTAATTTTTGAATTGGTTTATAATCCTGCAAAATTAAGTCTCGTGCTTCCTGAAAGTTCTGGAGCGACTTTTAGTATTTTGGAAGATGGGTCAGGGATCTTTAGAGCGAAACTGGAGAATCTCTCTCATTATATGTTAAATAGTGACTGGATTCAGATTCCCTTCTCTTGAGAAGAGGATCAGCTTGTTTTGTCTGAAGCGATGCTTTATCGTGGAGAAAACTCTGTCTCTCTTAAGATCGGAAATTTAAGTTCTTCTACGGAACATTCTCTTTTACCTTAGTTTTTCTTTTGATGAAAAAGGTATTCTTTGTATTTTGCAGTGTTTTTCTAGGTTTATTTGGGATGACAACATTTGCGGTGGGAAGTCCTGATAGTTTTCAACTGGAGGTAAATCCTGCAACTTTTAGTGTGAATCAACCCGTTGATCTTACGGTAAAAGCAATTAAGAATGGGCAGATTATGAAGGATTATGAGGGAAGGCTTTTCCTAGAAATTGAGTGAGATAATTTGTCTCGTTTTGATTATACTTTACCTGAAGGAGGATTTTGAGAAATGAAGCTTTCTAACCAGGGAGTGAAAACCTATTCTAAAGGATTACAAGTAAAAAAGGCTGGAACTTTTGTCTTGAAAGTCGAGGATCTCGCTTCAGATTCAGCTACTGGAACGATTACGATTATTGTCTCTTCTGATGATCAACTTAACCTTAAAAATATTCAGCTTCTTTCTCCTCTTCAAAATGGGACAGAAACAGAGAGTTCTATGAGTGTGATGGCAAATGCTCCAGAATTATCAAACTCTCGTATTCAGGTTTATTTGAATGATCTAATGGTAAAAGAGGCAATGACTGATACTAATGGATTATTCAATGAAACCATTCCACTTCAAAAGGCGTGAACAAATGTCTTGGAGCTAAAAGCTGTTGGTTTGAATAATCAGATTGTTGGACTTTCGGATAAAAGAACTTTTCTTTATCAACCATTGTCTGATAACTTATTTAAGTCAATTACCATGACTCCAAATCAGAACTTAAAAATCTGAGATAAAGTTCGTTTTGAAGTTTTTACCGATGAACGTGTGAGTTCTGCGAAACTTTTGTTTTCGTGAGGGCAGGAGTATCCTTTGGATAAAGAAAAGGATGGGCTTTTTACTAAAAATGTTACGCTTACTACTACTGGAGAAATCATTGTGAATACAGAACTTATTGCTTCAGAACTTACTAAGAAGTATGATAAGGTATTGTCATTTTCTGTTAGAGATCATATTAGAATTGGTGAAGTGAGAATCAGTCTTAATCCTAATCTTGCTGGGACTTTAGATCTTTCTTGGGTAACTCTTGGAGGAGAGAGCTCTGATTATGCTGTAAAGTATTGATTGGAGAAGGATCAATTGATTGCTACAGCCTTTACTAAGAAACCTCAAGCAACTCTTTCAGGTTTTTCTTATGGGAAATCGTATTTTTTTCAAGTTTTGGCAACGACTCCTGAGCATGTCCCTGAAGGACTACCTTCTCAGGTTATTCAGTTTGATATGCCAATTTTATCAGGAACTGCGGCTACGGTTGATCCTCTAACAAGTGGCGGATTACCTTCTGATCTGGCTGATCCTGCAGATCCAGATTCTCATGATGTGGCGGTTTTGGATAAGCCGCTTTGTGTTGTCAAGAATATTCAGTTTTCTACTAAGAAGATTTGAAATAAGCACTATCTCGTCCGAAATAAAGTAGCGAATGTGGAAAAATATCTGATTTATAGGTCTGAATATGAGGATGGAAGTGACAGGCAATTTGTTGGGGAATCTCTTGTTCCAAGATATGAATATCCTTTTGATAATACTGCGGAGGAGGATATTTATGCCTATTATGACGTAGAGGCTATTTGTAATGATGGTGAACAGCTTGTTTTGACTAAAGCAGAAAAGGTAAAGGTTGGTCCTTTTGAAGATATGTTGATGATTCTTGCTTGTACTTTCCTTATTTATTTGATGTATAGGCTTTATCACTATAATGCCTAGCCTGTTTGGCCGTTTCTAGAATTTGGAATATTTTATGCTGATAGTAAAGTTTAATGAAATTAAATAAAAAATATCAATTTCATCATTTTTGATCTCAAGATTCCTCTTCTTTTGATGGAGTTTTCCCGGTTTTTCTCCCTTCTTATCAGACTTTTTCTCTTCAGCAACTTGGAGAACTTATTGTGCAGGATATGCAATGGAAGGCGATTTCTCGTTCTCTGGGAAAGCACTTGATTCCTTTATTTATTGATGGTGCACTCAATAAAGATTTTACTAAAGTATTGTCTTGAAAAGCTTCACTGCCGTATCTGGCGAGAGAGAAAAGGCTTTTGTCTGTTTATAGGAAAACACAGAAAAAAAATCAGACTTTACTTTCTCAATTTTTTGGTAATGAGGGTATCCATTTTTGTTCTACGTTTCAGAATGAGAGGATTCAGAGGCTTTTTGATCTTTTGGAGGAGAAACAGGTTTGGAAGCTTTCTCAAGAGCTTGTTTGGTGGGATGTTACTACTCAAACGATCGTAGAGCCTGAAGATATTGTTTGGAAAGAAGAGATGCAAACACAGCTTGAGGTTAAGTGTTTTGTGGAGACGAAGAATGATGTTTTTCCGCTCGTTATTGATGATCCTTTGCAGCTTTTTTCTGATAAAGGAGTACTTGTCCATCCGAATGATAAAAGATACAAAAAGCATATTGGTAAGAAAATTATTATTCCAATTCTTAATAAATCGATTCCTATTTTTTGAGAAGAAGGATTGGATACTGTTAAAGATAATGGGATTTTTAGATTGAATCCGCTTTTGTGAGAATATCATTTGAGGAAAACGCAACAGTATCGTCTTTCTTTGCAAGATGAAGTTATTGATGAAAAGGGATTCTTTGTTTCAGATATCCCAAACTTTGGAGGGAAGCCAGCTTTTGATTTTGTTGAAAATATTACTGAAACACTTGCTACGATAGGAAATCTTGTTTCTAAGCAGGAGGTCAAGAAAAAGATTCCTTATTCGAGTGAAACGAATACTCGTTTAATAAGGAGAATCGTTCCGGGTATTATTTTGGATTTTTCTCAGTATTGAGACGATTTTTGAAAGTGGATGGAGAAGGAATTTCCAGAGCTTTCGGAAGAAGGGCGTCAGCCATTTTCAGTGAAATTGTCTTTGGAATCCAAAAATATTTACCAGCCTAGATTGCCGTTTCTTTGTAATGTTATGACAGGAGAGGTGAAGTGTTTTAGGTATCAAATTTGTGAGAGGAGCTGTTGATCTGCTTTTTCAAGTTTGCTTTTGTATCTTCTTGTTGAGCAGCAACTGCCTTTTGAATTTTCAATTAGTGACCTTTTTGATGCGCTTTATTTTATGTCGGAAGAGAGGTGGAATGCTTTTTTTGATCAGTTTTCTTTTTCTGTTTCTAAGGAGGATTCTTTGTTTGCACTGATGAAATCTAATGATCATGAGATGATCTCTGAACGCATTGGATTAGAATTGGAAAAAATTCCATGGCTACAAATGGACGCAAAGGGGATTTTCCGCTTTCTGCCTACTGTATTATTCTGAGATCAGTGGACGCTCCATTCAGTTGCTTGTTCTGATGTTTTTCTTCAGTCTTTGGCTTGTTTCTCTAGGAGTCAGGGTTTGAAGCTTTTTTTTCAGCAGCAGCAAGTATTCCCTTTATTTTTGGAGTATTTTCTCTGGTTTAGTTTTGTGCTTCAGGAAACTTCAGGTTTTTCTTTTGTGCCTTTTCATGCTGATGAGAAAGCTTTCCCTTTACAGGCAAGGTGGGAAAGTGAGCTCTCTGATTATGGTTTAGATGTTCTAAGATTTACTGTTGCTCGTGGAAAAGCTTATACAGAGTCAGATTTGGCTGTTTCTTTTGAATATTTAAAGCATCTTTGGAATCTCTTTAAGGTGCTTTATGAGCAGGGCGTTTTTGAGTTGGATGAGGTTTGAGAGCTTCGTCTCTGAGGAAAAGAGTATTGGCTTTTTTCTCAGTGGAAGGAGTTATTATCTGCGTTTGAGGAGAAATTGCCGTGAGAATCAGCCTTTTCTGTTGCTTTATGATCCTTGCAAGACTTTACCAGAGGAGCATTTTCTTGGTATCTTGCCTTGGTAAAAGGGGAAGCTCATGAGGAATCTAGGTTCTTGGCAGGTCAGTTGTTTTTATCTCTTTTGGAGCTCTTATCTCCGATTATTCCTGAATTTATTTCGATGATTGAAGGAGTCTTGAAATTTCCTGTAAAGTCTGTGGTAAATCGAGAAGAACTTCATGGTGTGAAAGATTATAGGACCTTCCTTCTTTTTGATATATTGAAGGGAATTCAGCTCAAGAGAGCGGAGCTTCAGCTGAAAAAACATCTTCCTATTGCGTTGTGTGTTCAGGCGAATTCGGATATTTTACGTTTAATAGAATCTTATGAATCAAGCTTGAAATTGCTTTTTAAGACGGAAGAAATTCAGTATTTAGCTGCGAATGAGGTTTTCCCTTCAGGTTTTGAAACTTTTATGATTCTTGATATTACGGTTGGAATTAAAGCTCTTGATCTTCCTCAGCATGAGGATTGTTTGGCGCAGCTTGAGAAGGAGCATACTTCGAAATTGCAGACCTTGGAATATTTGAGGTCGACTTTAGTAGCGCTTTCTTTGAATCCTTTGGCGGATCCTGTAAAACTTGCTGAGAAGGAAAGTGAACTTGATCAACTTAAGTCTGAAATTCAGGAACTTGAGCTGAATATCAAAAAAATGAAGGCTACAAAGAAGGTGTAGCGCTTCTTTTTTTTATTTTTTTGAGGGAGTATTTTTGTCGGTTAAGGTTTATGCTTTCACTTCTTGCATTTCATAAAATTCAAGCATATCTCCCATTTCGATTCTCTTTCCGATTTTTACTTTCATACCGCAGTCTTCACCCGCATTTACCTCTTTTACTTCGTCTTTATTACGGTGAAGAGAAAGGATTTCTCCGTTTGTCAAAATATCATCACCTCTATAGACTCTGAATTTAAGCTTATTCTTGACCTTCCCTTCTTTTACTCTTCCTCCAACCGTCATTTCTCTCGTTTCTGTGTGGAAGACACCAAGGATTTCAAGCTTACCGACGATTACTTCTTCTTTCTCTACTTCTACCATTCCGAGGAGTAGATTATTGAGGTAATCTGTGAGTTCATAAATAATATCAAAAACTTTCATCTCAATTTTGAGCCCTTCAGCCTTCTTTTTGAGAAGCGCATTCATAGAGATATTAAATCAGATCAATAATGCCTTTGAAGCTTGGGCTAAGGAAAGATCACTTTCTCCGAAATACCCTACATCGCTATGTACCACCTTGATTGTCACATTCTTTGGAAGCGAAAGTCCATCAACGGCCTGCTTGAGAGCCTCCAAACTAGAGGAACCATCAGCCTTGAGGATCAGTCTTAATTCTGATATCTTTGATTGATCTCCAGCTTTTAACTGAGCTAAGAATTGTTGTAAAGCTCCATCTCCACTCGCTTTTGCTTCAGACTCTTTGATAAAGGCAATTCTCTGATGAGCTTCTTTTTCGTTGGAAACCACCTCAACAATTCTTCCTGGCTCAGGAAGCTCAGTAAATCCAAGGATTTGCACAGGTTCTCCTCCCATGATTTTTCCAGTGTTTTTTCAGAGTCGGTTCTGCATTCTCCTTACTTTTCCATAAGTATTGTAAGCTACGATAATATCTCCGTTCTTCAAGGTTCCAGTCAGCACGATCAAGCTTGCTACCACTCCTTGTTTTGGATCTTTGTAAGCATCTACAACTACTCCTACCGCTTGTCTGTCAGGATTGTATTTGAGCTCAAGCATTTCAGCCTGTAAAAGTACATATTCTAGCAAAAGATCAATACCCTGACCAGTTTTGGATGAAACTCCTACGAACGGCGTTTCTCCTCCCCAATCTTCAGGAGTCAGTCCATATTTGGCAACATCAGCTTTAATCTGATCCATATTATTTCCAGGCTTATCAATTTTGGTTACGGCAATAATAATTGGAACGCCAGCGGACTTAGCATGGTTGATAGATTCAATAGTCTGTGGCATGACACTATCATCTGCTGCAATCACGATAATCGCAATATTAGTCAGCTTTGCACCTCTAGCTCTGAGTGAAGTAAAAAGCTCATGTCCTGGAGTATCAATGAAAGTAATCTGCTTTCCATTATAATTTACCATAGAAGCACCGATAGATTGCGTAATCCCTCCGGCTTCACCTCCTGCAACGCTCGTTTTTCTGAGGTAGTCCAAAAGCGAGGTTTTTCCATGATCTACATGTCCCATCACGGTCACGATTGGCGCTCTCTCAACCTGTTTTTCTGCCTCTTTATCAATATCCAAAATCTTCTGCAAATCTCCTTCCATAAAACTCTGTACATCAAGCGTTGCTTCTTTCTTCTTAAGAGTAACTCCTAAATCTTCAGCGATCAGAGAAGCTGTATCAAAATCCAAACTTGCATTCAAACTTGTCATAATTCCGTTGGCAATAAGCTTTTTCATCACTTCTGGAAGTGGAACTCCAATCTTTTCTGAAAATTCTTTTACCGTAATATTTTCTTCAATGGTTACCTCAGTTTTCTTGACGAGATTTGATGAAGTTGTAGCTTCTTTATGAACCTTCGCTGTTGGTGTTGGAGCAGGCTTCTCCTCTTTTTTGAACACTGGTTTTTCTACCTTTTTTGGTTCATGCTGCTTTTTTTGTGCTAGATGATCAGCAAAAAAATCTAATCCACTTCAGCTTTTTTGTGGTTTTTCTTTGGCTTTGTAAGTCCCCTGTTCGATAGAGATATTTCCTCCTGCCTTTGACGCAGTTGATTTTGGTTTTTTAGAAGATTCTACTGGACGAGGTTCTGCCCTTGCAATAACCCTAGCGTTTCTTATTTTTGGTGCATCTGAGAAAGAAAGCTCTTCTTGACTTGTCTCTTTATTTGAAGTAAGCGGGATTTCGATATTATGAAGCTCTAAGCTATCTTCATCTTCTCTTTCAGGTTTTTCTGCTTGTTTGGTAAATCCTAGTCCAGAAAGGAAGCCTCCTCCAAATCCTATTTCGTCTGATTTCAAAATTTTCCCATCATCAGCTTTTTTGGCAGGAGCGGCTTTTTTAGCAGCTTTCTTTGGAAGCTGATCTATGATATATTTCATTTTTTCTAGATTGCTATCAGAAATTGTTGCCGTTTTTGCGGTCAATTTTTTGCCCATAACTTTTTCGTATACTTTGGCAAACGTGGCTTCATCTAGGTTTAAATCTTTCTTTACATCATCAATACGAGGCATATTCAGCGATAGTCAGCGATAAAAGGTTCAAAAAAAACTGACCTTAGCATATGGAATTTGCCTCTATTTGCAAGGAAAACTTTGGTTGCCTCTGTTTTTTCAGAAGGAGAGTCTGATTTTTAAACTTTTTAAAAGAGCATACGTTCTGGTTAGAGCGATATTTATTTCTGAGAAGAGTGAGGTTTACTAGTGCTCTCAGAGCTCAGAGGTAGAGAATTTATGCTCGAAGCGGGCTATGAGTGGAGCGATGAGAGGTTTAATGAAAATCCCAACACAAAGGAATATCCCAAAAAAGCTCAGGAGTATGATCATATTAGGAAGATACACTTCTCGAAGGATACCTCATACGGCTTCTCTCATCGCGATGATTGTGTAGGTGAAAGGGAGAAATGGATTGATTGCTTGAAAAAAGCTACCAGAAAGTTCTATCGGGAAGGTGCCGCCAGCTCAGGAAAGTTGGAGCACCAAAAAGACGATGACGATTGCTTTCCCTGCACTTCAGAATGTGGCGACGGTGGTATAGATAATCATCGAGAAAACCATTGCTGCTCGGAGCGTGATTCCTATGAATGCTCCTTTATTAGCGACATAAGCGTTTAATAAAAACATATCTCCCAAAGCAACAATTATTCCCTGAATGAGTGCTATGCTGAGGAAGAGGAGCCATTTCCCTAGGTATTTTTGATAGTGTTTGCAGTGGTAGAAGGTTTTCTCTCTGATTTTGGTAGTAAAGAGTGAGATATTGAGCAGAGAGCCTACTCGGATGGCTAAGGTCGTAAAGAAAGGCGACATTGCAGAGCCGTAATTAGGGATTGAAAAAAGCTTGTTTTCTACAAGCTTTACCGGTTCAGCGATAAACTCAGAAAAACGATTAGGGTCGAGGGTCGCTACCTCAATGAAATTTTCTAGTTTTCAGCTAGCCTTCAGTTTTTGGAGGTGCTGATCAATCGTGCCGACATCCTTCTGAAGTTTGGGGAGTTGCTCTTGGAAGGATCTGACTTTTTCTGTTTCTGTATTGATGATCCTTGCACCTTCTTCAAGGTTTCTCTGGAGATCAGGAAGTTTATTCTCGAGGCGGTCGAGTTTTTCTATTCCGCTGGTTGAAAGATCTTGGGTGTGATTCAGGACTTGACGCAGTGCAGGTTCAATATTTGTTTCATAATCGTCATGGAGATCATCAAGGCTTGTGCGGATGTCCTGACGAATGCCTTGGATCGCTGTTTGGGTATCCTTGCTAAAGTCGATGACTTTTTTGGCGTCTTGGGAGAGTGGTGAAAGAGTCGAACGGATGGAAATGCTTTTTTGACGGAGGAGATTGAGCTTTTGAAGTAGTTTATTGAATGGGGCGGTCAAAGGCGTATGAGGAAGGAGGTGATCAATCATATCTCTTCGCTGGGTGATTTTCTGAGCTTGTCTTCAAAGCTCATTTTCGAGGTTGGTGAGTCTTGTGGTGATCTGGTTAAGATCGTTCTCGAGGTTCTGGTTTTTCTGATCGGCATAGCTAAGGATAGCAGAAAATTCGCTATCAATGCTTCTAGCGATGTCTTGGATTGAGGTTTTGTGGGTTTGGATTTGATCTGGGACGGTATCCAAAAGTTTGAGGCTTTGCTCGGCAAGTATCCTGCTTTGGTAGAGCGCTTCTTTCCCTTGATTGATGTCGTTTTTGAGGGTTGGGAGTTTGCCATAGGCTTCCTCGAGTTGGTATCTCGAACGGTAGGAAATCTCCAGAATTTTATTGATTTTTTCATCAACGGCATCTAAACTTTCACGAGCATCATGCACGAAGTCGATCAAACCATATACGCTTGTTTTGCTAGCTTGGAGATCGAATCCCACGAGATTCAATTTTTCCATCAGGACGCGATTGACGGTGTCTATGAAAGATTTTTGGATATTTTCTTTGATCGTCGTTGCTCCTTTGCTGGTGATTTTTGGGGCTATGGCGTTGAGCTTCTCGTTGACGGTATAACGGATTTCTGGGTGCTGAGGAAACTCATCAAGAAGTGTGATCATCTTTTCGCTAAATCCGCTTTCAATCACAATAGAAGCATAATATTTTCCTAGCTTTGTCCCTTGGTTCGCGATATTTTCATCGACAAAAACCCAACCGATATTTTGATCTGTCTTTAGCTCTTTGATAACTTCTGCTCCAATATTGATAAATTCCTCCTTAAAAACAGCTCCAATATCATTATTGACGACGGCGACCTTGATGCCTCTGGTATTCCCATAAGGGTCTCGGGAGGATTTTAGGTAAAACCAGGCATAGAGCGACGGCAAAATGCAGAGTCCTAGCACAATGACCATTGCACCTCGATTGGAAAAGATACGTTTAAGATCAGCTGTATAGATACGTCGGATTCGTTTCATTGGTTTTCATAAGAGGCAAAAATTTGTTCTAGAGTATAGTGATCTTGGGCTGTGTGTCAACGAGGAAGAAAAGTCTGATTTTTTTGACTTTTGTAAGGTTTTGAAGTATACTTGAGGAGATTTTTTTATTTTCAGTTTGCTTGAGATGCTTGATGTGAAAAAAGAGGCAGCAACGTTTAGTCGTTTGTCGCACGATGAAAAGCTTGAACGTGTCCAAAAATGGCTCGGAGAGCTGGCGTATCATTATGACTATTTTCGTGATTTGGACAGCTATGTGCATATCCATAAAGCATCACTTGATGATGTGTTTTTGGAGGCGATGTTTCAGATTATCCTCAATTTAGCCAAGGAAGTGGAAAAGCTTTAATTTTTTAAGCGAAAGAAGATGAGAAGTATGGAAATTATTAAAAATGATGAAGCGGCAGATAAAAATCTAAAGTCTGAACTGGATCAGTTGGTCGGTGGGATCAATATCAAAGAAAAAACTGAAAGCTTTGGTGCGATCGATCAGTTTTTTGAAAAGTTGAATAGTTTGCCAGAAGAACAGAAAAATTTAGCTCTTCAGCATGCGCTCGGTAAGAGCAGTCTTACGATTGATGGCTCCAAGAAGAGACTTAGCTCGTTGGCGAAACTTTTTGATAAGGCGGTCGATCAGGAGAATGGAACATGGAAAATTAAGCTGAAAGAGGGTGTTTCTTTGCGTGAGTCGTCCTCGTCTGATGTAGTGAATCCTGAGACAAGCAATTCTTGAGCTCAAGATACTACTGAAGCTTCTGGCGGAAGTGATTCTACCCCCAAAAACTATGTAGAAATTGCGAGCGCTAATGATCCAGAGGCGATGGCAAGCAAAACTCCTGCTGGAGCAGGTGAAAAAAAAGATGAAGCTGTTCAGACGACTCCAGCAACGGAAGAAGAACCGGATGATGCTGATCTTGAGGGGCTTAGTGCTGAAGAGCAGGAACTTGTTTCTTCGCTACTGAATGAAAAGAACTCACCTTTAGCGTATTTGATCCAGAAGAGCTCGAATTTTGCCAAGATTCAGGCTCAGAAAGGATATGGAGCGGATGCGGATACTTTGCTAGAAATTCGTGAAGATAAGATTCTCTGAAATCAGACGAGAAGGGCTTTATCTGGATTATTAGATTGGATTGGATCAGATTTATCATCTCTTGAGCTTGCGTCCTTGACGCCGGAAAGTGTGTTGTCGATCGGGTTCATTTCTCAAGATATGAAAGAAAGAATTGAGGCGAGCGGACCCGAGAGCGTGAAGTCGCTCTGGAATGATGAAGCGTATGAGCGTAAAAGTCCTTATACCAATAATTTTGCTTTAAAAAAGAAAGAAAATGCTGATTCTGATCAGCAGGTAGTTGATGGGAAAACAGATGTTGTAGATGAAGCTTTGCAGCAAACTCAACCAGGAGTAAAGAATGAGGATGGAGAGAAATCTGCTGAATCAACGAATGAGCTTAAGCGATGGCTTGTGGATGGTATCCCTCATGAGATTGTTAAGGTTTCTTCTTTTGATCAGCTTCCAAAGGATGAAAATTGAGCTTATAAGTGGTCCGCTTCGCCAAAAAAGCTTTATAAGGTGGCGGTGGCAGGCAAGATCTTTCATTGTTTTGATAATGGGAAATGTCAAGATATGAAAACGGAAGAAATGGCTGATACGAAAGATGTTGTAGCTAAGGTAGCGAAACTTCAGGAACAACTTCCTACGAAGAAAGCGAATTTAATCAAAGCGTTTGAGCTTCAAGAAATGGATGGAGGCTATTATACCAGAGAAGGAGACGCTTCTCTTTATACGATTGATGAGGTGGGGACGCTCTATGCTGCTGGACCGCACTGAGTGCAGAAGTTTCTCTGAGGAAAGGCTGATCATTGGGAGGCAGCTAAGGTAGAGGATTTGCCGTCGAGAGAGTTTCTCAAGACAGCGACGATTAGGACTCTAGGATTGCAGCAAACGGATGAGAACTGTTATGGAAAGGAATGATGGAGCGGAGTTTTTGCTTTCGACAAGCAAGGAAAGCTTTGTTATGCGGGGATTGATCGTGGAAGGATGGTTTTTGAGGGATTAGAGACGCTAGGGCATTCGGTTCAAGAACCTGCTAAAGATGTGGTAAAAGATTCTTTGCAAATGCTCTATACCCCTCTTTGGGTCCCTCAGCCTGTTGAAAATCAAGTTCAAATTCCTTTTAAACCGCAAGTGACGACTCCTCAAGAGCCTTGGGCTGCTCGGGATTCGGCCGATTTAGAAAAGCCTTGGAAGGAGGTTGCAGAACTGCCTGAAGAGGTGAAGTATATTGAAGTGTAGTGTTTTAGGTTTCGATGAGTTTTCGCTCGCCCTTCTCTAGCCCCTCGAGGGTTCGGGATCAGATTCTGAGTCTTTTGAGGTAAATGACCTTATTCCTGACTGCTTCGAGCATCCTTTTGACCTGATGATATTTCCCCTCAGTGATGGTCAAGAGGATTTTCTTTTCTTCAAGTTGCTGGACTTTTGCTGGTTTGGTAAGGTAGCCATCATCAAGCTGAACTCAATTTTGGAGCTGTTCGCAGTCTGTGAGGTTGATCGGTGATTGGAGCCAGACTTCGTATTCTTTTTCTTTATCTCGTTTTGGGCTGATGACCTGATGAATAAACTGCCCATCATTGGAAAGCAAGAGGAGACCCTCTGTATCGACATCAAGTCTTCCTGCAACATGCAGTAAATGCACATAGGGGCAATCAAGCATCTGCTTTTTGTAGCTCGGATAGCCAAATTCATCTTCATCTGAGGAAATATAGCATGCTGACTTGTAGAGAATCGCATAAACTTTTTCAAGCACCTTGATTTCTTGACCAAAAACTGTCAGCATTTCTCCACCAAAAAGCTTAAAGTCAGATTTTTTCACGCTTTCTCCATTTACGAGAATGCTTCAAGATTTACAAGCATTAGTAGCTTCTCTACGGGAAATTAGTCCGAGATTTGCGAGGTATTTATCAAGTCTTTGCATAATTTTTGTCGTGGTAAATGAGAAATTCTTTAGTCTCTCGTGATCATCTTTACGAACGTATCAGTCGGGATTTCCACATTTCCGATAGCTTTCATTCTTTTCTTTCCTTCTTTTTGCTTTTGGAGGAGCTTTCTTTTTCTACTCACATCTCCACCATAACATTTAGCAAGGACGTCTTTTTTGATAGCAGAAATGTTTTCACGAGCGATGATTTTATTTCCAAGTCCGGCTTGAAGAGGAATCGCGAAAAGATGTTTTGGGATCAGCGTTTTTAACTTTTCTACTGCTTCACGACCGAGATAATAGGCTTTATCATTATGCACAACCCAGGTTAAAGCCTCCACTTTCTCATTATTGATAAAAATGTCAAGTTTCACGAGGTCATCTGCATGATAACCTTTAAATTCATAATTCATCGTTGCGTAGCCTTTCGTCGCAGATTTCAAGCGATCATAAAAATCAACGATGATTTCGCCCATAGGAAGCTCGTAATGCCAAACGACTCTCGTTGCATCCAAGTATTCCATATGCTGGAGTTTCCCTCTATATTCCTGACAAAGTGCCATAATATTCCCTGCAAATTCTTCAGGTCAAACAATCTCAACATTTCAGATCGGCTCTAAGATTTCCTCAATCATTCCTTGATCTATCATATCGGAACCTGATTTTACGACGATTCGTGGTTTCAAGAGTTCAGCAAGTTTCACAAAAGTCTGCTCCTCTAGCATGGTTCCGAAAATCCCTTCGGAGTGTTCTAAATGTTCCTGTTCTTGTGCGGATAGCGTCAGATTTTCTTTTTTGAGAATTTCACGATAAAGCCCAGTCTGAATCAGACTTTTGATATTCATTCAAGTTTTGATCAGCGGAATGGAAAGGTTTTTGGACTTTACAAGATACATTACGGTTGGAATCGTGAAAATCGTATCCAGACCATACTCACGAAAAAGCCTTTCCTTGATGATATCCATATGGAGCATTCCGAGAAATCAGCAACGAAACCCAAGTCCAAGTGCCTTACTATCCTCCATTTCATAGACAACTGCACTATCATTGAGCGAAAGCTTCTCGAGGCTATCTTTGAGTTTGTCGTATTCAGTAGAGTCAATCGGATAAACCCCGGCATACACATAAGGAGTGACTTTTCTAAAACCAGGAATCAAATACTGATGATATTTGCTGGTTCCTTCGTTTTTATTGATCTTGGTTTTGATGATAGTATCTCCAATTTTCACATCTCTTACCGATTTTTGTCCGGTCACGATATAACCAATTTGCCCTTCATTAAGGCTTTTGTCGGCTTGATATTCTGGAGTGAAGTGTCAGACTTCTGTTGGTTGGAAGCTCTTTTCTGAGTGGATCAAATAGACCTCGTCTCCTGCAGAAAATGAGCCATCTACGACTTTTACATAGGCGAGAACTCCTTTATAGGGATCAAAGACCGAATCAAAAATCAATGCTCTAGAAATTTCAGACATTTACTACAGAAAAAAGCTAAAAAAGCTGATCTAAAGCTAGTGAATTGCTCTGGATTTTCAAGTAAGATTTTTTTGTTTTCTCTGAAGAAGGAATGTTTTTTGTCATAAAATGTTGAAATGGCAAGTTTTATTGTTATAAAAAATCAGATTTTATTATTTTTTATGGAAAATTCGGTTATAATTGAGTTAAGTTTTTTATTCATCAGTGTGATCGAGATGGGGAAAAAGAAGAAACAGCATCAAACGAATTCTTCCCTGTACTCTTCTGCTTTTAGTATTGAGCAGCAGAGGGCTCTTTCTGTAAATAAGGCGATCTCTGGTCATGTTGATGAGCTTGCTCAAACGCTTTCTTATGCTCCAAACTTTGATATTGATTCTACCTATTTAGACCTGGAAGCAAGGTTTGATTTGTCAGCTGGGAAGGGAATCCTTCGCGATATGATCCAAAATAGGAAATCTGTTGACTATGGGACGGTGAATTTAGAAAATTTTTCGCTAAAAGAGATCGAGTCTTTGAAGTTTTGGGAAATCCCTACGTCGAATCCACTTTTTGTTGCTAAGCTTGAAGAAGCAGTGATTCAGTGCATTATGGATGAGTTTGCGGCTCAACTCAATCCCGCAGAACAACGTAGTTTTACGGAGGCTGTTCGCTATCAGAGCAAGAGTTTGCCTTCTTTTCTCAGTGCGGTTCATGCGTATGTTGATTCTCTCAATAACTTTCAGCAAAAATCTCGTTTCCAAAAAATGATTCAGACTTTTGTGCTTCCTTTGAGAAAAGTTGTAGATCAGCTCGAGGCTTCTGGTGAAGATGTGACTGCAATCAAGACAAAAGCATTTCAAGATCGAGCACATGACCAACAAACTTTTGTAGATGCTTTTGTGAGTAAGGCGGTGATTTCTAGGCTTCACAAAAGAGTTGAAGAGATGAAAATTCTGATCAATGATGTGAAAGATACTTTTGATAAGGTTGTCCCATTCTTTCCTACGATTTTTCAGCAATTCCCTGCGAACTTTTCTGCTCTTTTGCAGGTGTTTCCGGAGAAAAAACAAGTCTGTGATGCACTTGAGAAAGAATATGAAGCAGCGACTTCTGATGCGGAGCGTGGTCCTGTAGCAGAGAAGATAAATGCTCTTTACTGGGAAGTCTATCTTGCAGATTTAACTACTAAGGAGCCAAAACTTTGTGCCTATCTGACTGAACTGAAAAAATCAGATTTTGACTTTTCTCTCTTATCTGATCAGAGCGGATTTTTCTCTCTGATTGCTCAGATGAGGCTTAAGCAGATGAAAGATGGCACGATGCTATCGTTTTTTGGTCAGGATACTGCTGCTTTTGAGAAGTTTTTTTTGGAACTTTTTGATCTCAATACGTCTGAAATTTCGGTTAGTGGCTATACGATTCCTGTAAAAAAGAAGGTACTTCCGGGTAAGCATTGGGATTATAATAATTTTTTTTATGGACAGTCCTCTGATGCGCTTCCTCTAGAGTATGAGCTTGATATTAGTAAAGTTCCCCTTTCTGTAGAGGATAGATATTCTTTTGAGAGGCTTTTTGCTTATGGGAAAAATCCGGCTTCTCCGGGGATTTATTCCCTCAAAGGTAATGATCTTGGAAAACTCTTGTTCCTTTTTAAACTTGGGCAGGACAATTCTATCTTTGACAATTATGATCCGAGAGCTTGAAAACAAAAACTGGATTCATTCTTTGATAATTTATCTCAGCTGGGAAAATGTACTGAAGATTATCGTTTTTCTCCGCATAAAAATAAACCTGGAGATTCTACAGATCCTTCGGATTCCTCTCCTGAAGGGACTCCAGAGTCGAATGAATCAGTATTGGAGCAGTTTTATGAAGCTTGGAAGTCTCTTGATGGGATGCAAAGTGAGGATCCAGATTGTGGATTTAAGGAAGGTGCTAGTATTTATGGTCCTTTGTGTGAAAGTGCGCTTCCTCCTTATGAAAACTGAGGAATGTCGTGGATTAAACTTGAAATTGTGAAGGTCAATAAAGATGCAACGCCACCTACCTTTAAGTTAAAGTCGAGCGGAACAGAGAGAAAGCTTGCTGCTTCTGAAGAGGGAAAAGAGCGAACACTGACTATGACCAAAGAAACTCTTGATAAGATTCGTGGTCTTTTGAATGGAGGAGCTAAGAAGGTTTTGAGTGAGAAAAAAGATTTTAATCAACAGATGGAGGATTTTCAGAAAGCAGGTCTGTGTGATAGAAATGTCCTGTGAGGTGCGGTTTTTGAGAATGGGAAATTTACTACGACTCCAAATCATTTGGATGGAACTATCGGTCAAAAAGAGGATGTTTCATATTTCTGAACAACAGATGTTGTCCCAGATCCTCAAAGCTCAAAAATGAAAAGGTATTCTATTCTTTACAAGGTAAAGCATAATTCTGATCATACTTTTTCTGTTTCTTCTGAATTTGTGAATCTTGAGTGAGAATCTAAGACTCACGAACACAAGATGAGTTATTCAGATTTTTTATTCTTTTTAAATGAAAAAAAGCTTGCTCCCAAGACGCAGTCTCAGGTTGAAGAGGAGAAACAGGCTAGACAAGATGTGATCTCTTCCCATAATAGAACGTGGAAATTTACAAGTATTCAGTCTGTACTTTTTGCTCGGAAGACTATCTGGAAAAATCTGAATTCCAAGCTAGATGAATATCAAAAGGAACAAAATGAGGATTTTTTGGGATATTTGGTGACAGGAAAGGGAATTTATAAGTGGATATGAGATACTTTGGGCTTTATCCCTTCGATCAAAGATGCTGCCAATAAGCTCCATGAGGAAGCAATGCTCAAAAAAGAGTCAAATACTTGGAAAGGAATAGAAGCTTGGATCAGTAAATTTGCTTCTATGCAAGATTATGCTAATCTTTTTGAAAAAGGGTTTGATCATCCGAATGGCTATACGCTAGATGCGATCATAGGAAAAGGGAATACCTTGCAGAAGATCCTTCTTAGTGGGAAATCTGTTGTGGAGAATGATGGACTGAGACCAATTATGGCAGCAGCAATGATTGCAAATCTTAGGCAATGAAAAGGACTTTATCGTGGGTTGTCAGGTAAGGATAATCAGGGACTTTGGGTGAAGTGTTTGCTGGGAGATGCACACTATAAGAGATATAGAGCTATTAGAGCTGATGTCTTAAAAAAGATAAAGTCTGGAGTGAAGAATGCTGATCAGCTTCAGGATCAGCTAAAAAAATCAGAGGTGGATTATATCGTCAATAATATCAGAAATGCAAATGGGACAGAATGAGCATTTGGTTCCATTGATGACGAAAATAAGCAAATTCTTAAGCAACTTTATTCGGATAAATTTGCAGGAGAATTAGAGAAGGCTGCTAATGAGACGACAGGAAAGTCTGCTGTAGAGTGAGCGTATGGAAAGATTTCTCATAATAATTTTGCTATAGCGTCTAATGATTTCAATAGATTTATCAAATCTGGAAGGATTGAATCTGCACTCTGAAATCTCAAAAAAATGTGAGATTTGGCGAAAACTCCAGAAAATCATAGGAAACTTAAAGTGGCTATGACCTATGTGATGCTCAGTGGTATCATGAATAGATATGGTGATAAAGCAATGAGGAAGTGGTTTGATGCTTTGGCGAGAAACTATATGTTGCCGACGACGCTTTTTGCTAAAAAATGGGAAAATCAAGAATATGCAGCTCATCTGCTCAATCAAGTATTCCCTCCGTCAGATGAGAAAAATTTTGCTCGTGCTACAGGATATAAATTATCAGATTTTGATAGCTCTTCTCCAAATATTCCTTATGGTAAGTTGCTTGATAACTTGGATGTTCGATGGAGAGAAAATGAGAAATCAATTGATAATTATTTTCTCAAACTTAAGAGCTCAGATTGTGCTCATGATCCTATCCTCAAGGCGGTTCAAGATAAACTTTGGGAGAGGAGTGAGGATAACCTCGATGGAGACTGGGCTTCAAATAGTGGACTTACGGGACACTATGCGATGTTAGCATCTCCTTCTACTATTTCGAGGAATAAGTCCTATGATCGTTCATGATTTACGGGGAAAGATATTGATGAAAAAAATGATAAAGCAGATTTTTGGACGAAGGTTTTGGGTGACTTAAATAAAGCAAAAGATCTCCCAAATGCTCCGGAATTTTTGCTCAGACAGTTTGTTACATGGTTTGTCCCTTCAGAGTGAGATTATTCGGAACTTATTAGCTGGATCAGAACTGCTAATGCAGTGAAAAATTATGGGACAAAAAAGGCTATCTCGTTTACAATCCATGATGAATCTGATACGCAAAAGACTTTTTCGTCTTCTCCTGCTGTGTATAAACAAACAGATGCAAGGGATGTTTTGTGGTATCTCTTTAAGGGGAAAGTTTTGAGATTAAACTCTGCTCCTCCTCCAAAGCAGTTTGAAGATGTTTTGAATTTTTTCATGGATTATTTTAGTAAAGGTCATGTTTTGTCAACAATTGCTCAGGATTCTACCTTGAAAAAGGTGTTTTGATCTTCTTTGCCTAGTCTTCCTGCTAGTAAGCCGGTTACCCTTCTTCCATGGCAAGAATATAATAAATATGTTGATAAGAATAATCCTTTCGCTACTCAAAATGATCCAAAATGACTAACTCCGGAGCAAAAATGGGATAGAGCTTGGAAAAGGAAATACTATAGAACGGGGCTTTTCTTGAATGAAAGCTTGGTTTCTATTCAGAAGAAAATGAATGATATGGGAATTTCTGATACTCCTCAACTTTCCGGATTTGATGAAGATTTTTCTAGCAATATTTATAATCAGGTTTATCACACCGCAGCTTAGCCATGTGAGAGATATTTTATTGCTTTTATTCAAAGAACAACTTGCTTTTCTTAGAGAAAACTCTAATCTAAATCTGTTTTTTAGATTGTAGCTTATGTGGGATGAAGACTTGGTTGTTATTTCAGTATTATTGTTTTCTTGGGCGATGTGCTAGGCATTATCTCAAGAAATATCAGGTCTTCACTATAGGAATCAATGGAAGTGTGGGTAAAACTTCATGCCGTATGATTATTCATCAAACTTTGCAAAAAGCACTTCCTGAGCAGAGAATTTATACTTCTCCAAAAAATTTCAATGGCGAACTCTGAATGTCCCTCTCTATTCTCCAGGTAGAACGTCGAGAACCTCAGATTTTTACTTTTCTGAAAACGGCGATTCTGGCACTCAAAAAAGCTTTTTTCTCAAAGAAGGAGTATGATATTATTGTGCTAGAATATGGGATTGATAGACCAAAGGAGATGGAGTTTTTGCTAAGGATTAATAAGCCACATATCGGCGTTTTTACGGCGATTGATGCGGTGCATTCTGAGCAATTTGGTAATCCAGCCGAGATTGCTAGAGAAGAAGTAAAAATGGTAAAAAATACGAGAGAAATGGCTTTTCTGAATTTGGATGATCTGTATGCTCAGCAGCTCGTTTGAACCTTAGGTGTTGATGAACTAACTTATCAAACTTTAGGCTCAAACAGTAAAGCAAATCTGACTTTTTCTGATGAAAAGCTCCATCTTGACTACAAAAGTAAGCATCTTGTGCTGACGGATCTTGCTGTGCAGCTCAAGAAACAGTTGTTAAACCTTCAAACAAATCTTTTGGGAAAACCGAATTATGGCTATCTTACGGTTGCTTTGGTTATCGCTCAGGTATTGGCGTTGAAGTTTGGTGGGAAGGAGCTCGATATGGATGCTTTGTTGGCAGAGCCTTTGATTTTTCAGCTTCAGCCGTGAAGATGTTCGATCTTTGCTGGGAAAGAGGAGAGTATCCTTATCGATTCTACCTACAATGCTTCTCCACTCAGTATGAGAAAGCTCATTGATACAACCTTGATCCTTAATAAGTCGCTTCCTCAGCAGAGAAAGGTGATGCTGGTATTGGGAGATATGAGAGAGCTTGGAGATTTGACGGAAAAGGAGCATCGTTTGCTGGCTGGCTATGTGCAGCAGAGCGCAGATTTTGTGGTGCTACTTGGGACTTCAATGCATCAGTTTCTCGCTGACGAATTAGAAAAAATCTGATTCTCTCCTTCAAGGTTTCAGCTTTGTCGTTCGGCTTCTGAGGTAGGGAAATTTGTCCTTGAATATCTCAAAAAATCAGATGAAAAGTGGATTGTCCTGCTGAAAGGAAGTCAGAATACGATTTTTCTTGAAGAAGCTGTAAAGATGCTCCTAAAAGATTCTTCTGAAGCTCAATTCCTTACAAGACAATCAAAACGATGGCTGGAGAAAAAAGCGTAATTTTTGTGCCTTTTCTTTTCGTCTAAACTGTTTGATTCGTTTTTAGCTTGCTATTTATAGGCATTTCTGTATCTTTTTTATTTTATATTATTAGTGTTTGGGATGAGATTGACAAAGATTATTGCTACCGTGAGAGATGATTATGATAAAGGAAAAATTGTAGAGCTTTTTGATGCGGGAGTTGATGTTATTAGGATAAATTTTACTCATGCAACGCCGGAAACTTCGGAGGCTTTGATGTCTGAAATTAATAGATTGAACGCAGAAGAGAAAACTCATTTGAGTCTGCTTTTGGATACCAAATGACCGGATATCAGGACGGGCGTAAGAACTACTCCTTTAGCGATTTCAAAGGATCAGATTTTTCATATTGTGATTGATACCTCAAAACTTGTGCAGGATTCGGATGTTTTCTGTGATTATCCTGGCATTTTGACTGATGTTTCAGTTGGGCAGGAAATTATTATTGATTCTGGGCTTTTGGTCGTGGTCGTGGTTGAAATTACTTCAGATTATTTGGTTGTAAAAGCGCTTAATGATGCAGAAATCTGATCTAAGAGGCATATCAATCTTCCTGGAGCGAAAATTTCACTACCAGCGATGATAGAGAAAGATAAGACGGATATTCTTTTTGGTATAAAGATGGGAATTGCTTATGTGGCGGCTTCTTTTATCAGAACTGGGGAGAATGTTCAGGAAATTAGGAGATTCCTTGATGAAAATGGAGGAACAGGTGTGAAGATTATTTCTAAGATTGAAAATAAGGAAGCTTTGGAAAATTTGGAAGCTATTGTCAGATATTCTGATGGTATTATGATAGCGAGGTGAGACCTTGGTATTGAGATGCCTATTCACGAATTGCCAGTATATCAAAAGAAAATTTTGGATATGTGTTTTGTCTATGGGAAGCCTGTGATTATTGCTACTGAGCTGATGAAGAGTATGGTGAGCAATCCTTTTCCAACAAGAGCTGAGGTAAGTGATGTGTATAATTCTGTCATTATGAGGGCGGATGCCGTGATGCTTTCTGATGAAACGGCCGTTGGAAAATATCCTGTGAAAGCTGTTCAATATATGGAAAAAACTGTAATGGAGGCAGAAAAGACAACCTGCAATAAGCACAAGGATTTTGACCTCCAGTCAGAAGAAGAGTCAGAAGTTTTGAAAAAGGCGCTTGCACGTTATGCTCTGATGCTTGCGGATGATATTCATGCAAAGGTCGTAGTAGCTTTCTCTTATTCTGGGAATCTCGCGAGGTATTTGTCTGCTTTTAAGCCTAATCAACCTGTAATTTCTTTTACTACAGAGGAGGTTTTGGACTATTCTTTTGGGATTAATTACTGAATTATTTCTGAAAAAGTTGAAAAATTCTGATCCCATATGTCTGAAGATCAGGATATGGCGATTGCTGTTCTGAAGGAGAAAGCTTTTGTGAAAAAAGGAGATTATGTACTTTTTGTCGGAGAAAGGGTCGCTGGGAACTCTCGCCAACCACAGTTGAGGATTGTTCAGATTGATCAGTAATTTTGCGTGGTAGAAAGTCAGTATTTTTACTTGTTTTTTTGCTTTGAATTGATAAAATCACCTTTGCTATTTTGGTGCTATGTTTTTAGTTTTGTAGTGAGAGGAAAATGGATTTTGATCCAAAAAAGAATTATTATGAGATTTTGGGTGTGAGTGAAACTGCTACCGCAGATGAGATAAAAAAGACCTTTAGAAAGCAGGCTGTGAAGTATCATCCGGACCGTTGAGGCTCAAAGGAAAAGTTTCAAGAGATTAATGAGGCTTATCAAGTGCTTTCTGACGATCAGAAAAGGCAACAATATGATATGTATAGGAAAGGAGGTTTTGGAGCTTGAGGCGTTGATTTCTGAGGTTTTTGAGGAGGAAGTGGTGCTCAGTTTGACTTCTGAGGATTTGGCGATATTGGCGATCTTCTCTGAGGAATGTTTGGAGGCGGGTTTTGAACTGGAGGATCGAGGACGAGAAAACCAACGAAAGGGACAGATTTGGAGAAGCATATTGAGATTACTTTTGAAGAGTCCTATCTTTGAGTCAAAAAGAAAATTGCCTATACGAGAGCGGTAAAAGCAGATGGGCTTACGGAAGAGACCTGTTCGCACTGTAAATGAACGGGAAGAGTGAGTCAGCAGGCACAAACTCCGTTTTGAGTATTTCAGACTCAGACTGCCTGTCCTGAATGTTCAGGTTTTTGAAAGCTTTATAAAAAGGATGGGAAAGTAATTTGAAATGGTGGACTGGAGAATCGCAAAGAGATGCTAGATTTGGAAATTCCCGCGGGTATAAAGTCTGGAAGTTTCCTCAAGTATGCTGGAAGAGGAGATGATGGAGCAGGAGATGTCCCTTCTGGAGACCTTTATGTGAAGATTCTGGTGCAGGATTCCACGAAATATGCGAGGAAGACGGATGATCTTTATGTAAAAGCTGAAGTTTCCCTTTTTGATTTGGTCCTGGGAGGAGAGGTCGAAGTGCCGCATCCTGAGGGAAAAATGACCGTAAAAATCCCTAAATGAACTCAAGTAGGGCAAAAAGTCCGTGTAGCGGCAAAGGGTTTTGGAGAAAAGGGGCTTTTCAAGTCAAAAGGAGATATGATTGTAGAACTTCAGATTTCTGTGCCAAAAAAATTGACGAAAGAGCAGGAGAAGCTTCGAAAAGAGTTGCAAAAACTAAATTAGAAAAAATCTGATTCAAAAAAATCCTGATCAGTTATGAGTCAGGATTTTTCTTTTTTTAGCGTTGTTATGCTAATTTCCTTTTTACAGCGATCTTGCCATCAAATCCAATCTGAGAAATCACAACATTGATCGTATCTCCCACTTTGAAATGTTTCTCTAATCCATCAGTAAACTTCTGACCGAGATTGGAGACATGGCAGAGTCACTGCTTTCCCTTTGGAAGCTGAACGAAGAGTCCGTAAGCTTCTACTCTTACGATTTTTGCGTCAAAGACTTGTCCTACCTCAAGATCTGTTGCAATCTCAAGGATCAGATTTTTAGCCTTTTCAATCATCTCTTGCTTTTCATGGGTAAGGAAGCAGGTTCCGTCATCTTCAAAGTCAATCTTGATATTATCGCACTGCTCAATAATTTTATCAATCATCTCTCCACCTTTTCCAATCACTTCCTTGATTTTCTCAGGATTGATTTTGAAAACATGAATCTTTGGAGCATATTCTTTGACTTGAGCTCTCGGAGTTGGGATAGTTTCTAGCATAAAATCCATAATCTCTTTGTATCCCTCACTTGCTCTATCAATGGTTTCATGCACAATATCCAAAGTCAGACCTTTGAGTTTTGTATCAAGCTGGATTGCGGTGATTCCTTTTTTTGTTCCTGCGACTTTGAAGTCCATATCTCCAGTAAAATCCTCAGTTGCCATAAGGTCATTAAGCACCATATGCTTGGTTACTGCTCCATTTTCATCGGTTTCAGTCATCAGTCCCATCGCAATTCCTGCAACTGGCTTCTTGAGCGGAACTCCAGCATCCATAAGGGCAAGGGTAGAACCACATACGGATCCCATACTGGTTGAACCTCCAGAGCCAAGACATTCTGAAACCACTCTGATAGAATATGGGAATTCTTCTTTGCTTGGAATCATATTCTCAAGCGCTTTTTCTGCCAATCTCCCGTGTCAGATTTCTCTTCTTCCTGTTCCTCTCATGGCTTTTGCTTCTCCAGTAGAGAAAGGAGGAAAGTTATAGTGATGGAAATATCTCTGCTCAACATCGTTATGCTCCATATCTTCAAGGACAAGGTAATCTTTTGGACCTCCGAGTGTTACGGTAGAAAGCACCTGCGTATCTCCTCTTCGGAAAAGTCCAGAACCATGTACTCTTGGGAGATTATCCGTCTCACAGAAAAGCGGTCTAATATCTTTGATTTGTCTATCGTCAATTCTCTTTCCTGTAGCGAGAGTTCTGCTTCTGATGAAAGCTTTCACCGTATTGAAAACTGCCATCTTGATTTTACTTTCTGTGTATTCTTCTGCGTCTTCGGATTCAGCTTTTTCTTTTGCTGCTTCAAGAGCTTCTTTTTCATACTGACTGAAAAGATCGTTGAAAGGTACTTTGGTATTTCCAGCAAGGGCTTGGAGTTTTTCATCGGTCAAGAGTGTTTTTACAAACGCCATCGTTGCTTCACTTGGCTTATTGAAAGTGATTTCTTTCTGCTGAATGTCTAAGGTCTTCAAGAAATCAAGCTGAAGATCGCAGGACTCATCAATTACTTTCTGTCCAAGCGTGAAAGCTTGTTTCATCAGCTCATCAGGGAATTCAAGCGATTCACATTCAATCATATTGATAGACCCTTTCTTTCCTGCTACCAAAAGATTTAATTCAGATTTTTCCAACTGTTCTTTGGTAGGATTTACGATAAATTCCCCATCAAGATAGCCGATCTGTGCTGCACCTACGGGACCATCAAACGGAATCCCCGCCGCCATAATAGAAAGCGAAGCTCCGATAATATCCATCACTCCAAGGCTGATTTCATGATCAAGTGCAAGTGGAGTCACGGAGATTACGACATCATTGATCATTCCCTTTGGAAACATCGGTCTGAGCGCTCTATCGGTAAGTCTCGCATAGAGAATTGCTTGATCAGAAGGTCTTCCTTCCCTCCTTCTATAGACCGCACCTCCGATCCTTCCAGCAGCGGAATAACTTTCTCTCATATCAATCATGAGTGGTAAAAAGTCCATGCCTTCTCTTGGATTTTTCTCCATTACGGTTGAATAAAGAAGCACATTTTCTCCCATTTGCATTCTAATGCTTGCATCAGCTTGCACTGCAAGTTTCCCCATTTCAAAGGAAACTGGTTTTCCATATCGCACGGTAGATTGACGAGCGATTTCAAATTGTTTTTTTACGACCATCGTTTTCGTAGAAAAAAATAAAAGTGATGAGCTTTCCTCAGCACTATTGAGACAGATTAAAAGGCAAAAAAATCTGATTACTTTTCTGCGTTTTAATCCACCACGGCGATGAGGAAATGGCTCACGAAAAAAGTATACACTTTTCAGAGGAATTTGCAACAAAAGAAAAACTTAAGAATCTTCTTGCATTTTTAAAGGAATCAGCTAGAATTTCATCGTTTATTTGACTTAGAAATAATGAAGAAAACCTCACTTCTCCTTACCATTATCCTTGCTCTTGTGGGGGCACTTTTTGGGGTATGGTATTTTAACGCTTCTCATTCTTGTAGTGCTTTTTCTGCTGAATGTAAGGAAATCCCTGTTCAGCAACAGGAAAAAATTTATCTCGATGTGCGTGAAGAAGAGGAATGGCTTGCTGGGCATGTAAAGGGAGCTTTACACATCAAGATGAGCGATATTTTTGCTGGGAACTATGAGCAAATTCCACAAACTGAACCAGTCTATGTATATTGCCGTTCTGGGCGCCGTGCAGGTGAAGTGATTCGCTTTTTAGAGCAAAAAGGATTCAAAAATCTGATCAATGCTGGTGGTTTGCGTGATCTAGAAGGAGTAGAAATCGTAGAAGGTGAGTAATTTCTGTTTTTTAGTAAAAAGTTTACTTGACAAATAGTTTTGTATTTGTATATTTGTCTTGTTTTTTTATGATTTCTTGTAAAAAATAAGAATGGAGAAAGATATCTTTTGAGCAGAGCGTCAGGAATGAACTCTTAATCCTGAAACTTCAGAAACTGGAGGAACTAAGGAGCCAATAGCGAAAATACAAGAAACCACTTCGGGAAATCTTAAAGTTCTTCTTGACAGAAGAGAACTTAAAATTACTAAGATTCATTCTACTGCAGAGGTGGATCCTTCAGCACAGATTGATCCTTCTGTTGTTGTTCCAGAGGGCTGTGTTATTGGACCAAATGTCGTTATCGCAAAGGGGGCTGTTTTAGGAAGAAATGTTCATATTTGAGCTAATGCGAAAATCTGAGAATGAGCGGAAATCTGGAGCGGATCAAATATCTGAAATAATGTGCAAATCTGAGAATGAGCGGAAATCTGGAGCGGATCAAATATCTGAAATAATGTGCAAATCTGAGAATGAGCGAGAATCTTATATGATGTGCAAATCTGAGAATGAGCGTCGATCTACTATGGAGTACAAATCTGAGAATGAGTGGAAATCTGAGAATGAGCGAAAATCTCGAGCGGATCAAATATCTGAAAGAATGTGAAAATCTGTGACGAAACAAACATCTGAAAGAATGCGAACATCAGAGAATGAGCAAACATCTGAAAGAATACGAAAATCTGGGACGGAGCAAACATCTGAAAGAATGCGAAAATCTGTAACGAAGCAAACATCTGAAAGAATGCGAAAATCTGAAATAATGCGGATATCTACTATGAAGTACAAATCTGAGAATGAGTGGAAATCTGAAATAATGTGGAGATCTGTAACAAAGCAAACATCTGAAAGAATGCAAAAATCTGAAATAATGCGGAGATCTGACTTGATGCGGAAATCTGAAATAATGTGAAAATCTGAGATAATGTGAGAATCTGACGACGAGCGGGCATTTGAGAATGAGCGAAAATCTGAAAATGAGCGAGCATCTGAAATAATGCGAAAATCTGAAATAATGCGGAAATCTGAGATAATGTGAAAATCTGACGATGAGCGAGCATCTGAGAATGAGCGAAAATCTGAGAATGAGCGGAAATTAGTCCTTATTCTGTTATTGATGGAAAAGGGAATTTGATAGGCTATCTTCAGCAAGAGGAAAGAGTAAATGAGGAGCAAGAGGAAAGAGTAAATGAGGAGCAAGAGGAAAGAGTAGATGAGGAGCAAGAGGAAATAGAGAATGAGAAGCAAGAGGAAAGAGTAGATGAGAAGCAAGAGGAAAGAGTAAATGAGGAGAAAAAGAGCTTTTGGAAAAGACTTTTTGGAAAAAATGATTAAAAAAAAGAAGAAGACGATCGTCTTCTTTTTTTGATTTCCCTCTTTTAGAAGGAGAATCCGCTAGAATAAGCTGAAAGATAAGGATTGGATCAGATTTTTTCCTAATGCCTGTTGTTTGTTGGAATTTTCCCTTTAATATTGGATATATTTGTCGTCAAAGTTTTCGTAATAGCTTTTAACTCTGAGCACATCGCCCTTCATTACCCCTTCAGATGAGAGTATCTCTAGGAATCCTTTGCTTTGCATTTGTTGTCGAAAATATTGCTCGGCTTGGTCGTTGCCTCGAGGGATCATAAAGACGAGTTTGCAGATTTCGCGGTTTTGGAGATACCAAACTTTTAGGAATCTACTCTCTAGGGCTTCAAGATACCCGTCCTTGATCAAGGTTGGTTTCTCTTGCTCGGTGATCTCTGTGACCATTGTCAAATCTTCAGTTTCAGGTTCTTCGAAATAGGTTGTTTCATCAAAGATGCTTTCTGGGTGATAGCCATTGTCTGGAGTTTTTTGGAGGAGTTCAGCGAGTTTTCTTAGCATTTCTCCGACTCCGTAATAAGTTCCCGCTGAAGTTGTGAAGGTATTTTTTTCTAGGATCTCCTCATCGAGTCTCTGGAAATTATTCTCTCTCAGGAAGTCATTGAGCTCTGCATAGAGGGTTGTTTTGTATTCTCTGAGGATGGCTTCATCGTTTAGGAGGTCTCGTTTGTTGAGGATCAGGATTATTTTTTTGCTAAGGAGGAGTTCTTCTCTTTTTTTTACCTCGAGTATGATAAAGTTTCCGTCTTCTCTGAGGATGAAGGTAAGCTCTTCTCCTTCGCTAAATTTTTGCTTGATGTAGGTTATAATTTCGCTGAAGAGTCCTATTGTCTCTTTGATTCAGGCTTCAAATCTGGAAAGGTCGGCTACAAAGGCAAAGATTCTTGCTTTTAAAATGTGTCTGAGAAAAGCGTTTCCTAGCCCCTTTCCCTCAGATGCTCCTTCAATGAGTCCTGGGATGTCAATTACATTAAACTTGAAATCACCAACGGATACGGAGCCTAGATTTGGAACCAGGGTAGTAAAAGGATAATCGGCGACTTTTGCCTTAACATTTGCCATACAGTTGATAAGGGAGGATTTTCCCACGCTTGGGGAGCCGATCAAGCCTACGTCAGCGAGCAGTTGGAGCTCCAGAATGATTTCTTTTTTTTGTCCTGGTTCTCAGAGGAGGTAGAAGTTTGGATATTGATTAACGGCGTCTTTGAAGTGGATGTTTCCGTAGCCTCCTTCTCCACCTTTGAGGATTTCTACGCTTTGTTGGTCGTGTTCCAGTTGAGTGATGAGTTTTCAGGTTTCGGCGTCTTTTATTACCGTTCCGACAGGGACTATTAGTTGGAGGTCCTCACCGTGTGCTCCATATTGGTCTTTGGTCCTACCTGGAAATCAAGGTTCTGCTTTAAAACTTTTTTTATATTTATACTCAATAAGGGTGTTTTCGTCCTTTGATGCGGTAATGTATACTGAACCTCAGTCTCCGCCATCTCCTCATGAAGGGCCTCCAAACGGGATTCCTGGAGCTCTTCTTCCACTTGCTACTCCGTCTCCTCCTTTTCCAGATTCGATGGTTATTTTGACTTCATCATAAAATTGCATTTTCATTCTCAAAAGTAAAAGAGATTCTGAATTAAGGTAAAGATTGCTCCTTTAATTGCAAGGAGATTTAAGCGTTTGTCCTATTTTTTCTTGCAAAAGAAGAGGGAAGGTTTATAAAGGTTTGCTGTAGGTGAACTTTAAGAAGAGCTTTTATTTTCTCTCAGAAAAACTGATGAAGTTGATCGTTGGACTCTGAAATCCCTGACCAAAATATGAAAAAACCAGGCATAATATCTGATTTCATTTTTTAGATTTGTGGAATCAGCATTCCGCTTTGTGAGCTTGGAGCTATGAAAATAAATATAAGGCGGAGCTGATTTCTGCTGAATGGAAGGGAGAAAAGGTCTTGCTCTGCAAGCCGCAAACGTATATGAATCTTTCGGGAGAAAGTGTTGCTCCTTTGGCGAGGTTTTTTAAAATTTCAGCAGCGTCTATTTTGGTGCTTCATGATGAGATTGATTTCGTTACAGGAAGGATTGCTCTCAAATTCTGATGAAGTCCCGCAGGGCATAATGGACTCAAGAGTATTATTGAAAAGCTTGGGACGAAAGATTTTTGGAGGGTTAGGATTGGGGTTGATAGACCTGCTGTTCAAAGTCAAGTTGTTGATTGGGTTTTGAGCTCGTTTAAGCCTGAGGAAAAGCATACTTTGGAGGAGAAAATAGGAGAGGTTTTTGGATGTATTGAAGATTTTTTGTCGCAGAAAAGGTAGTTTTTTTGAGTGTGGTAATCAGTCTTTTCTTTTTTTATTGCGCGTAAGAAGCGTTGCTTTCTGGAGGGAAAACCTTACACTGAAGCAAGTTTATTTTTGATAGTTTCGTTATGTATGTTCCACTTCATGGGCGTAGCACCTATTCTTTTCTAGAGTCGGTTTGAACGCCTAAAGTTATCATCAAGAGGGCAAAGGAACTCGGATTTTCTACTCTTGCTCTCACGGATTTAGATGTTATGTATGGAGCGATTCAGTTTTATCAAGCGGCAAATGCTGAAGGGATTAAACCGATTATCGGTTTAGAAGTCGGGTTTGTCTTGAATGTTGATAATGCTCCAGCTGTTAATGCTATCGGAAGTATCTGTCTTTTGGCTAAAAATACTCAATGATATCTGAATCTGATGAAGATTACGTCTTTTGCGGGTCAGCAGGGGGTTGCAGGTAGACCAAAGATTGATATTACTCTTTTGGAGAAATATAAGGAAGGAATTCTGGTTTTCTCTGGTGGAGTGGACTCGTGGATTGCGAAACTTTTGAGTAACGGCGAATCGCTTTCCAAGGCGGAAGAGATCTTTACTATGCTGAAGGATAAGCTCTGAGCCGAGAATTGTTATCTTGAAGTTATTGCTCAAAATGAGGCAAAAGAACCGGAGATTGAGAAGATTAATAAGGCAATACTCCTCTTGGCGGAAAGGGTTCAGGCTTCTTGTCTAGTAAGTAATATTTATATTTACCCAAAACCAGAGGATAAGCCTACGCAGGAATTGGCTATGGCGATAAAGGATAATCTAAAACTTTATGACCCTCAACATAGAGTCTTGACTACGGAAAATCATCTTATGACGGAAGAAGAAATTAGAAAAATCTGTCTGGAAAACGGCTATTCTGAAGCTCAAATTGATTCGTGGATTCAGGTGACGGAGAAGATTGCGGATCTTTGCTCTCTAAAAATTGATATGGGGCAGCTTCTTTTCCCTAAATATGAAGCGGAGCCTGAGATTCTCGAACTTTATGAAAAAAATAAGGATCAGCTAATTTGTGAATAAGTCTGGCTTTTTGTGTATGCATGCTAAAAAGTGCGCTTGTTTACGAGAATATATTGATCAAGAAAAAACTGATCCTGCTTCTTGAGGACCAGCTTTTTTGAAATGAAAGGTAATGGTTTTTTTATTCTTCGATGGATTGACTTTCGTCTTCTATGATCGCTTCCATTTTGCTTAAAGCTTCATCATCGAGGACCTTTTTCCCCAGTCTCATGTCTTTGATTTTTGACTGGATTTCTTTTTCTAGGTTGTTGCGGATTTTCTCTTCTCCTTCAAGGTATTTAGCGAGTTTTTCTTTTCCTTGGAGTTTTTCATTTCCTACGGTATAGAAAGCTCCTGCTTTGGAGATAAGCTTTAGAATTAATGCTGCTTCAATTATATCTGTTGTTTTGTCGTATCCCATACTCCATTTTATAGGTAATTCAGCAGTTTTGAACGGAGCAAAGATTTTATTTTTAGCGACTTTAATTTTGGCGAAATATCCGATTTGCTCTTTGTCCTCCATGATTTTGTCCCCTTTTCTGATTTCTATTCTCTGAGAGGCAAAGAACTTGAGTGCATTTCCTCCTGTGGTCGTCTCTGGATTCCCAAACATAATTCAGATTTTCATTCTGATTTGGTTGATGAAGATACACATTGTGCCAGTTTTTGCGAGGACGGAAGTCAGCTTTCTAAGTCCCTGTGACATCATTCTCGCCTGAAGTCCCATGTAAGAATCTCCCATATCGCCTTCCACTTCGGCTCTTGGAACAAGGGCAGCTACCGAATCAATTACGATCATCTTTACCGCTCAAGTTTTTGCGAGTTCTTCTGCGATTTGAAGGGCTTGTTCTCCGTAGTCTGGTTGAGAGAGAATGAGTTCTTCAGTATTGACTCCTAATTTTTTAGCATAATGTGGGTCTAATGCGTGTTCGGCATCAATGAAAGCTGCTGTTTCTCCTCTTTTTTGCATCTCTGCAATTGCATGGAGTGCAATCGTCGTCTTACCTGAAGATTCAGGACCGTAGATTTCAATCACTCTTCCTTCAGGATAGCCTCCTCAAAGGATCAAATCTAGCACATAAGAACCACTATGAGCAGTATTTACTAGTCCAACATTAGCATTGTCTCCCATTCTCATTACAGCTCCTTTTCAAAACTGTTTTTCAATGTTCATAAATGCTTCTTTGAGTGCTTGATTGTTTGTGTTTGCCATAAATATTTACTGTAAGAGTATAAAACTTTTTTTAGTCGTCTGCTCCAGCAAGAAATTTTTCCTGCCAGAGCGACTGAGATGGTGTTTAAGGAAAAAAAACAAAAAATCAAGAGAATTTTTATAATTTTTCATTGGGATTATTTTTTTGTCAAAAAAAAGTTTGATTTCGTCAAAAAAGATTTGACATTGTCAAAGAGATTCGTAGACTATGTTTATATCTTTATGTTGTTATCTGGAGTAAATGATCAACAAACATATTATTGGACTAAAAATTACCACAACAAATGAGAATAATCAAGCACAGGAAGATATTAATCAGCTCCGAAAAAGCTTTTATGATCAGGCAAATACTGAACTTCAAACCTTTAAAAAAGAGCCTTATACCTATGTTGTATATACTAATTATCAGGGAGATTTTCAGCAAGGTAACTATGATTGTTATCTAGGAATAGCCAGTGAAAGTACCCTTACAGGATTTGCATCTTGTGATATCAAGCTTGAGAAGTATCAGATTTTTGATTTTCCTTATGATAATCCTCAAGATACGCTTGAGGCACGAAAAACGATTTGGGCGGATCAATCTCTCAAAAGAGCTTATCTAGCAGACCTTGAAATCTACGACTTTGAGCATAATCGCCTACAAATTATTATCAGCACCATTGAAGACTAAAGTATTTTTATTTTTCCCTCTCTCTAATGCCTCGTTCTCCTTTATGCAAACTGTCGAATATCTGACCAAGGATTGCAGATAATCTGATAAAAATCTGAATTACGACACCTGAGGAATTTTTGGCACAAGATCCTTATGAAGTCTTTCATCAGTTCAAAATCAAAGTTGATCCTAGTCTTTGTCGCTGTGCTTTGGCGAGCATTATTGGAGCATACAAGGGAATAAAACGAAACCTGATTCGTGAGGACGCGATTAAAACTTATGAACAGCGTTATCCTGATGATGAACGAAAAACTGACCGAAAAGGTTGTTAATCTAACTTTTATCTCTTTTTACTATAATAGTATGGCAACGAATAAACTTGATGATTTACAAAGAAGATTGATCGCATTTTTTAAGCAAAATACTGAACAGGAAGGACTTACTCTCAGGGAAATTGCAAATCAAGTTTGAGCTTCTCATCCACAAACTGTTTTGAATAAGCTCAATCAGCTCGTTTTTAGAGGGTATTTTGTCAAAGATGATCGTTGATATAGACTTTTGAGAGAAGATGTAGAAAATAAGTTGGATGATATTGTTCAGCTTCCGATCTATGGTTTCGCACAGTGTGGAAATAAAGGAAAGCAGATCATTGATGAATATTCTCAAGAAAAAATGCCGGTAACAATCGCATTTATTGGGACAAGTGATATTAAAAATTGTTTTTTTGTCAGGGCAAAAGGGAATAGTATGGAGCCGAAAATCCAGGATGGCGACCTCGTTCTTATTCGCCAACAACCAAGTTATGATGAATCAGATTTCGTTTTTGTTTTGCATAATCAGCTTCCTAAGCTCAAAAAAATTATCAACCAAAATGGGCATTATATGCTAGAATCAATTAATCGTTTTTTTGATAAGGTGGAAATTGATCCGTATGATGAGACGAAAGTGATCGGAGTTGTGAAAAAGGTTATTAAGAGTATGTAGAGGAGGAGCGGGACTTTTTTGATCTAGGAGGTTTTGTAAGATAATTTGTCTATTATTAATTCTATTTCTGTATGATTATTTTTAAGAAAAAATCTGACTAGGAATGTAGTCAGATTTTTTTTCCCAAAATTATTGTTAGTTATCGATCTTTTGGTGGATAAGGTCCCTTACTTCCTGATATTTTTCCAAATTTTTAAATACAATTGGTTTATCGTTCCCCATTTGTATTTCAATTCCTCCAAATCAGAGGAAATGTTTTATTTCTACAGAATTGATTTTTTTGTATTTTATATCTTTTTGTTCCCTGAAGAGGAGGCCTTTTTCAATTACTACACTATCATCTTTAATGATTAATTTTTGAGCAAACACTTGTAGTGTTTTTATTCAGCAAATAACCAAAAGCAGCCACCAAATAAATGCTCCCTGAAATGCTAACCCAATACCTATATCTCCCGTCAGAGAAAGAGTATGAATGATTCCAAAAAGTGCCCATAGTCCTGTAGAGATGAGCCATCCCCACTTAACTAGTGAATTTTTACCATAAATCCTCCCCATCAGTTTAAAAAACAAATATAAAAACTGCCATAGAGTAAAACTGAAATTCCTCCAAAAAGAAAAAGTTCCTATAATCGGTTCTTTTCTTATGAGGAATATTGTTTTGGCGATTGCTCTTTTATTTTTTTCCCCTTAAAAGTCAAGTATGTTTTGTCTTAAAAAATTATTTGAAATAGAGATCTACCAATATTATTGGTAATAAGAGGAGATCTATTTTTATTACACTTATGATATGCCCTCTATCATATTCTTCAGATAATTGGAGGGCTTCCATAAATTTTTATTCATCTCTATTTATTATTTCTTTCTTCGAATATTGAATATTCATAAGTAGTTTTTAAACTTTAGTAGGAGTAAGTATTATTTTTTTTTCGTGCAATTTACAGGTTTTTCCTTTCTGCGAGGAGTTCTGCTTTTAGGTTAGCTGGGATTTTTGGGTTTTTGAGGATAAATTGGAGCATTTTTTGTAGCGTAGGGAGGATGATCTGCAGGATCATTTGATATTTTTTTTCGGGAGTGGTGAGTGGTTCTAGCTGATTATCTCGTCGGAGGGTAAGTTCCTCGATTTTTTGTTTTTGCTCTGATGGGATTTCTCAGTGAAGGGCTTGGTTGAGGAGGCTTTCTGGATCTGCTTTTGTGAGGAGCTGTATGAAATAGAGGAGTCCTTGACTGGCAGCATTGTTTCAGAGATAGGTATTGAGGTGTTCTTGATAGAATAAAGCTACAAATAAAAGTTCCCTTTCTAGTTGGTAATGTTCGTGCTCTTGGGAGTTCTTTTGAGAAGATTGGGTCGCATAGAATTTTCAGTCAGTATTTTTGTATCTTTTGAATTGGGCGTCAAGGATTTCAAAGGCAAATCCCAGTTCTTCTGCTAAAAGGCTTTTATAATGTTCCTGGATAGTGAGGCTATTAACTGAGAGAATCATTTCGAAGAGCGTATTGATCAGCTTTTGTTTGTCGATGGGAGAGCTCATGTCTAGCTGTGAACGAAGATCTTGGTATACGGTGATGAAGCCGTCTTGAGCGGAATTTAAGCAGTGGTTAAATGTCTCCTTTCCTTCTGGTGTATTAGCAAGGTCATCAACGTCTTTTTGTTCTGTAGGGAGTTTTATAATTTTTGGGAAAAGGTTTTGCTGATAAAAGAGTTTGAGTGCTCTTTTGGTAGCTTGCTGTCCCGCACTATCGTTATCGAAGAGGAGGTAGATATTTTCAGTATATCTTTTCAGGGTTTTTACGTGGTCTTCGGTGAGTGCAGTCCCGGAAGTTGCAACTCCGATTGGCATATCAAGTTTTGCAAGTCCTAAAACGTCCATCTGTCCTTCCACAATGATCAGCTTTTCCTGTTCTCTAATAGCATTTTTAGCAAGGTTTAATCCATAGAGGATCTTTGATTTTTCAAAGGCTTTGTGTTCTGCACTGTTGAGGTATTTGGGTTTATCTTCAGGATTTAGCACTCTGGCAGAAAAACCTACGATATTTCCCATGGTATCTGCGATTGGAAAGGTAATTCTGTTTCTAAAAAACGCATAAATTTCACCATTAGCGTTTCTTTTTGCCAAAGATGCCTCTAAAATATCGTTGTCGTTGAAGCCCTTTGTCCTCAGGCGTTGGACTAACTCATAATAGCTTTCTGGTGCGTATCCTATTCCAAAACGTTCAATCAATTCTGGGGAAAGTTTTCTCACTTCTTGTAAATATTGCTGAGCTTCTGGATGATTTTGGAGGTTTTCTCTGAAAAAATTTTGAGCAAGGGTATGGATTCTTTTGAGCTTCCCTTTTTCGTCGCTTTCTGCAGCGTTTTTCTTCAGAGATGTATCGTATTTTTCAAGGTCGATATGCTGCATTTTTGCGAGCTCTTTGACCGCGTCTCGGAAGTCAATTTTTTCTATTTCTTGCATAAAGGTTAGCACGTTCCCTCCTTTTCCGCAGCCAAAGCATTTGAAAATCTGTTTTGTTGGCGAAACCATAAATGAAGGAGTCTTTTCGTTGTGGAATGGGCAACATCCAGAAAAATTACTTCCAGCTTTTTTTAGTGGAACATATTTTCCGACGATATCTACAATATCTGTGGAATGGAGAATATCATCTATGATTGGATTACTCACGAGAATACAAAAGCTACAAGGTAAAATTCAGAACGGGTTTTAGTATGCTTATTTTTGTGGGAAATGCAAGCCCTTCGTTGTTTTCTCTCCAATATCTCTTGCATTTAAGGTGGAAGACTGTAGAATACAAGTCGTTTTTGAGTCTGTTTTGATTTTTTGAAAGAAAGAAATGAAGTATCGAAAAATGACTAGTATTGGAGTTTTGCTGCTTGGTTTGGATCTCTTGACGAAATGGCTTTTTTATGATTTGCAACTTGGGGAGGAATTGCCTTTTCTTCATCCGACGCTAAATACCGGGATTTCTCGAGGGATCAAAATCTATCTTCCTTTTGTTATTTTGATTTCATTCGTAGGGATTGGGCTTTTCTTTTGGTTATGGAGACAAAAAAGGTTTGGAAATCTGATTTTTCTTCTTTTTCTTGCTGGAACGCTCGGAAATCTTGTTGATAGAGTGTTTTTGGCTGGAGTAAGGGATTTTATTAGCATTGGAAGCTTCCCTGTTTTCAATCTCGCGGACTGTTTCCTTACGCTAGCTGTAGGAATTTTGTGTCGAAATGAGATTTTCCCCTTGCAATTAAAAAAGAAAACCGTATCTTCGGGTAAAGTTTAATACTTTTTTTATTAATTATTATTATTACATGCAGTATCAAGGATTTGTGAAGTTTATCGGTCCTAAGGAGACGATTGGTGACGGAGTTGAAAAACAGACGATCGTTTTGGAGGAGAATACAGATAGAGAGTTTAAGGGCTCTATTGCGGTAGATTTCTTTAGAGATAAGATCAATCTTTTGTCAGATGTTAATACTGGAGATTTGATTACTGTTCACTTGAATGCAAGAGCTAATGAATCTAGAAATCAACCAGGAAGATGGTTCAATAGCATCACTTGCTGGAGAGTAGAAAAGGCAGGAGCGACTCCAAGTGCTTCTTCTGATGATCTTCCTTTCTAAGTTTTGGAATTTTTTTTGAGCAGAGAAACTAATACTTTCGGGTGTTAGTTTTTTTGTAAAGGAAAAAGTCTGGTTTTTGCCAGACTTTTTATCTAATGGGATGAGTATTCACTGCTCATGATCTCCATTTCAATCATATCATGATATTTCCCTCAGAAATACCAATGCTCTTTTCTTCTGCCAATTTCTTTGAACCCTACTTTTTCATATGCTTTTGCCGCTCTTGCATTATTCGCTACGACATTCAAATATACTTTGTGAAGTCCGAGGATTTCAAAAGCATACTTGAAGAGGAGCTGAATACTTTCTGTTCCATAGCCTTGAGACCAGTAGTTTTTGTCGAAAATTGCGATTCCAAGTTCGGCATTCCTGTTTTTTCGGTTGATTTCAAGGGAAATGGTTCCGATATTCTTTTCGCCCTCAACACAGATCGCGAAGACTCTCATATTGGGATTTTTGATTATGTGGTCATAATAGTCTTGTTCAGCTTCTTTGTAAAGCAGGCATCCGCCGACGCTTAGAAACTGCTGCACCTCTAGGTCATTCATATGGCGATACCGAAGTAGACTATCTTCTTTCTCGATGAGAGCAAGTGCAACCCTTTCCCCTTGCAAAATTTTTACTCTTTCTGTCATTATTTGTAGGCTCAAGAAGTAAAAGTCTGATTTCCCCGTTATGGAGTGACTCTTTGCATATCTCTTGGGAAGAGTTCTGCTTCCTTGATATTTTCGAGCCCAATAAGTTTCTGGACAAACCTCGCAACTCCGAGTCCAAATCCTCCTTCATCAGGCATTCCGTATTTAAAGGCGTCTAAGTAATGCTTCAATCCTGGATGTTCTGGATTCTGTCCCTGTGCGGTGATTTGATCTACCAATCTCTGGTAATTTACCTCACGCTGCGTCAAAGTGACGATTTCTACGCCTCTGAAAAGGAGATCTGCACGATCGGCAACTTCAGGATTTTCTTGATTTTGGTGGTGGTAGAATTTTGCATCTGATCGAGGGAATCCATCCACAACAACAAAGTCTGAATTCCAATGCTTTGCTGCGTATTCACAGATAAATTTCTCTTCTCAAGGTGCAAGATCTCTTTCATGGCGATAATCTTCTCCTGTCTCTTTGAACATCAATTCGTGTAGTGCGTCTACACTGATGCGAGGATAGTGTTCTACGAGGAGTGGTTTGGTTGCACCGAGTGCGAGGAGTAAGTCTTCAGCTTCAGCCCAGGTTTGCTCTACTACAAAGTTGGCAAATCTCTCTGTCATCTCAATCACATCATCAAAACTATCAATAAATCCCATTTCCATATCGAGCATCAAGATTTCAGACAAATGTCTGCTGGTATTACTCTTTTCTGCTCTGTAGGCTCTTCAGATTTCGTAGACTCTTTCGAAGATTGGAACCATAATCTGTTTGTAAAACTGAGGTGATTGAGCAAGGTAAGCTTTACGTCCAAAATAATCTATCTCAAATACTTCTGCTCCTCCCTCTGTAGGGAATCCGATAATCTTTGGCGAATTGATCTGGGTAAATCCATTCTCATCGAAGAATTTTCTCATGTTTTTTTCTACAATGGCTGCGAGCTTGAAAATTTTCATCTGTCTTGGATGTCTGAGAGCTACCACTTTGTTTTCTAGAAGAGTTTCACCATCAGCATGGATCTCATCTTTGGAAATATCGATAGGGCTTGGATATACTACTTTTCTAATGATGTTTACAGAGGCTCTTTGGAGTTCAAAGCGAAATTTTCCCTTCTGCATTTCAGCTACTGTTCCTTTGGCAGAAAGTACCGTTCCTAGTAAGCAGCCTTCAAGCTTTTTAATCTCGTCTGCATCCTCAAAAACTACTTGTGCGAGTCAGCATTTATCTCTCATGATTAAAAATGCGAGGTTTGCTGATACAACACGGACGGTATGCAACCATCCACGGATTTCTACGACTTCTCCTACGTGTTTTGAGAGGTCTGCCGAAAGGATCATATTTTTATACATCTTACTAATGAAGAAAATAAAACGCCTTAAAAATATAAAAAATAAGTCTTTTTTCAAGAGGGAAAATAAGAAATTTTACTTTTTTGCTGGAGGAATGCTGGTATGTATTTCTTTAAAGGATGAAATCCCTTTTTGATCTTTGTGGTTTCTGGGGTGAGACTTTTGTAGAATATTTATTCTTCAATGAAGAAGTATGTATATAAAAAACTCTCCTCTGGATTTGAATCCTTAAAAGGAGAGATCAATGTCTGCCTATTCAGACTTTTTTATTTTTATAGTTATTGATTGATGTATTCTTGCAATCAATAATTAGCACATTGTTGTTTGTTGTAATCAAGGTAGAGCGGATAACTACCTTTTTCTCAATTAATTTCTACCTCAATGTCTTTTATAAGGGTATTGAACCTTTCATAGCTTTGAGACTTGTTTTCTCTTTCTATTACTTTCTGTACCCCTTCTGTGAATTCTGGGATATTTTTAATTGTTCCTTCATTGCTATATTTGTCGATGGTCACTCGATATTCTAAAACTTTTGTCGCATAATCGGTTCGTTTGCGTCCTGAGTATCTATTGAGAGCTAATATTCGTGCATCTTTTCAATCTTTGGGATTATACTTGCTTTTCAAGAATCTTGCTGCGCAGTCTATAGCAAGTATTGGATGTCGTCTATCGTCGTATTTTGAGAGCTCTTTGAGATCTTTTGTTTTGTTGAATTTTTCTATAACTTTTTTTCATAGTTCTGTGTCTTTCATCTTTCTGCTATTTTTTGAGTTTGGATTTACTTGAAGGACTTTTAAGCCAAAATCTTCTGCGTTTGCACCTTGAATGTGAATGAGTCCAAGGCCTCAATCTGATTCTGAAATTTCAATTTTTTTCGTTTCTGAGTTTGTCTTTCGTTTTAAAATGTTTGGTAATGTCGGATCTCCTACTCACTCTTGTGCCATCATCGCCATAAGGAGTCATCTGGGAAGCTTGTATTTATCTTCTGCAGCATCTGTTATTGATTTTCGTCTTAGGCATCTTAGGATTTTTTCTGCTTTGGATAATTTACACTTTATCAAGTCTTCTTGTGATAGTTTTGGAAAATTTATAAAGTGAATCTTATGTTCTTTATTGATTTCATTATATCCGAGATCTATTGGCTTTTTTTTGAGTTCGCTAATTGAGAGTTCTTTTTTAGGGGTTGTTTGTTCTATTTTCTCGTGTGTAAGTGTATTTGGTTTTTCATCTTCACAGCTAGAAAAACTTCAGCTAAGACATAGTCCAAGTAATGCTGCTATTAATCCATGTTTCATCCTCTCTTGAAGACTGTTTAATTGTGCTTTTGTTTTTCAGAGATTATCTCTTGGAAACTCTTGATTTTCTCTGTGTGTATTTGTGTTCATTATTTTTAGTAGAATTGGATAAAGTTCCTTTTAAAAGTATAGTCTAAATTTTATAAAAAGCAAAAAAAACAGATTTTTTATCTTTTCTTATTTACAGAATGTATGATCTTTTGTTAAAAACTTGACAAAAAATTAAAAGTATGTTTTGTTGGCTTGAGAGCTGGATTTTAAGAGATGAATTGAGGTCTTGGAAGATGAAAAAAGATGAATTTTCCCTTGCAATGTTAAAAAAGTGCTAACTACCTGTTATCAATCTTCAAAAAAATAGTCTGACTTTTCCCTTGATTTTTCTTTGCAATACCATATCTTGTAGTCGAACGAAAAAATGAACACAAGATATTGTGAAAAGAGCGTTCGTCGGCGAAAGTCTGATTTTTTTTATACCTTTAATTTTTGCATCCAAATGAGCATCCAAAAAGTAATCAAGAGGAACGGTTCAGAAGTTCCTTATAATCCTGAGAAGATCCATATTGCGATCCAGAAAGCCAATAAAGAAGTGCCTGAATCAGAACAAGCGAGTGAACTCGTAATTGAAGAGGTGATTAGGGCTTTGGAGGCTTCAAAGAATGGGACGCTTCCGATAGAAGAAATTCAAGATTTTATTGAAATGCAGCTAATCAAGCTGAATAAAGCTGAACTTGCGAAGAAATATATCATCTACCGCTATCAGAGAGCTTTGATTAGAAAGGCAAATACGACCGATGATTCCATTCTCACGCTGATCAGAAATGATAATGTGGAGGTGGGGCAGGAAAACTCTAATAAGGATTCTGCGATCGCTTCTACTCAGAGAGATCTCATTGCAGGAGAGGTTTCCAAGGATCTTGCTCGTAGAGTTTTGCTTCCAGAGGAGATTAGAAATGCACACGATGAGGGGATTCTGCATTTTCATGATATGGATTATTTTGTGCAGCCAATTTTCAACTGCTGTTTGATTGATATCAAGGATATGTTTGAGAATGGAACGGTGATGAATAATAAGCTCGTAGAGACTCCAAAGTCTTTTCAGGTAGCCTGCACGGTGATGACGCAGATTATCGCTTCCGTAGCCTCTTCTCAGTATGGAGGTCAGTCTGTGAATATTAAACATCTTGGGAAATATCTTTTCAATACCAAGAAAAAGTATGAAAGAAAATATAAAGAACAATTCTGATCCGAACTTTCAGACGAACAGATTCAAAAGCTCGTAGAGATGAGAATTAAGGACGAACTTGCTTCTGGAGTGCAGACGATTCAATATCAGATCAATACTCTTATGACAACCAATGGGCAGTCGCCTTTTGTAACGCTTTTTCTTGAGCTTGATGAAAAAGATGAGTATTTGGAGGAAACGGCACAGATTATTGAGGAAATCCTAAATCAAAGGATCCAAGGAATCAAAAACGAAGCAGGAGTTTATGTTACGCCAGCCTTTCCAAAGCTCGTGTATGTTTTGGATGAATGTAATGCGCTCAAAGGTGGAAAATATGACTACCTCACTCAGCTTGCGGTTAAATGCTCTGCCAAGAGAATGTATCCTGACTATATTTCAGCCAAGAAGATGAGAGAAATCCAGGAGGGAAATGTCTTTGCTCCAATGGGGTGTCGTGCCTTCCTTTCACCTTGGAAAGATGAAACAGGAAACTATAAGTTTGAAGGGCGTTTTAATCAAGGTGTGGTAACGCTCAATGTTCCTCAGGTTGCAATTCTTGCGAATCACGATGAAAAGAAGTTTTTTGAGCTTTTGGAAGACAGGCTTCAGCTCTGTTTTAAAGCACTCATGTGTAGACACGAAAGGCTCAAAGGAACGCCTTCAGACATTAGTCCTATTCATTGGCAGTATGGAGCGCTTGCAAGGCTCAAGAAAGGAGAAACCATTGATAAATATCTGACTGGTGGGTATTCAACGATTACGCTGGGATATATCGGACTTTATGAGGCTTCAGTTTTGATTACTTGAGATTCTCATACCAAGTCTGAGGGGGAAGCCTTCGCGGTAAAAGTGCTTCAAAAACTCAAGGATACCGTTCTTAGACGAAAAGAAGAAACCTGAATCGGATTCGCCTTGTATGGAACACCAGCGGAAAGTCTTTGCTACCGTTTTGCCAAGATTGATAAGAAAAAGTTTGGTGAGATCAAAGATGTGACCGATAAGGGCTACTATACCAACTCTTATCATGTAGATGTGAGAGAGAAGATTGATGCTGTTAGTAAACTCGCTTTTGAATCGCAGTTTCAGAAGATTTCTTCAGGAGGATGTATTTCCTATGTTGAGGTGCCAAATATGCAGAAAAACCTTCCAGCCTTGGAAACGCTCGTTCGCTTCATCTATGACAATATCATGTATGCAGAATTTAATACGAAATCTGACTACTGTCACGAATGTGGATTTGAGGGGGAAATTGTAGTCAATGCCGATATGGAGCGAGAATGTCCGCAATGCCACAATAAAGATCAGAGAAAGATGAATGTGACTCGTAGAACTTGTGGCTATTTAGGAGAGAACTTTCGGAATTTAGGGAAAACTAAGGAGATTTCAAAGAGGGTATTGCATGTAAGTTAGAAAAGCTCTTTCACTTGTTTTCTTTAGAAAAAAGAATATACGGGACATTAAGAGTGTTTTGCTGCCTTTTATTTCTTAATCAATGTATTTTATTTTGGCAACATTCTTGTCTTAAATTCAAAATATTTTTTATTCTTAATAATATTCTGGTTTCTGGAACTGTATCTGCTGCGGGATAAGTTTGACCTTTAATGAATCTTTCTTTTATTTTAAATATGCTGATTATGAAGCCTAGAAAAATCATTGCTATCGGTGTAATAACTTTGTTTTTGTTACTAAGTGCTGGATTGATTCTCGGCTTTTTTTATCAGGATGTTTCTGATAACTATGAGGATATCCTTAGTTTGTCTACAGAAAGTCATAGAGGAGATCAGATTTCTACAAAAGATTTGATGGTTAGGTTTAAAAATGGGAGAGATCAGATTTCTTTTTTGAGATTATCAAACGATCATAGACCCTATTTACTTACCATTCCAGAGGGATTGGATTATCGTTCGTGAGGAATTACGATGATTGGGCATTTTGAAAATGTAGTGGGTAAAGATTTCTATTTGACATATGCCCCTTTCCCTCACACGCTACTTACCTGATGAAAAAAGTATTTTAATTTCTTGTTGGATCCTAGTTCGTTTTATTTTGATTCCCAAAATACTGCTTGTAGACGATTTAATGATGAAAAAAGATGTTTCTTTAACGGCAAACTAAAGGAACGAATACTTCGGGTAGTATCTGATCCTAGCAATGTCTATGTGGTACAAATTGATTAAATTGGTGATAAATGCTCTGTAGGAGTTGTTTTTGAAACTTCAGGGTAGACTTTTTTCTTGTTTTGACGAGGCAGTAATTATCGCAAAGAGTGTCTAGAAGGGAAGAGAGGCGTCTGATGATTTATACTTATTATTAATTTAAAAAAAGGGATTCTTATTGTATTAATATCCCTTTTTCTTGGAGATAGTCATAGCATTCTTTCCTTAATGGTTGTAATGAGGATAGGCTATGAGCATCACTATTTACGTATATTTTCTTTGCATGTTTTAGCATATAATCTAGTTTCTCTGGGATTGCACGTCCCTTACGAAAAGTTCCATAATTAAATTCCATAGAAATATCATAATGATTTGCAAGTTCTACGATTGGCTCAACCTCGAGATATTCTCCAAGTTCATTGGTATCATAAGGATGTCAAATCAGATTAATTTTTTCATGATGCTGTTCAATTGCTTTTAAAAGTCCTTGTGTTGCTGTTTCTGGAGCTGCAAGGTAGCCATTCCAATGCACGGAAAGGATTGTGAATTTTCCTTCTAGTTGTTGATTGGTGAGGCAGACGTCTCATTTTTCATTCAAAACATCTCCTTCTACTCCAAAGATTACCTTTACATCGTTATGGACATTTTTTCGGCTATTGAGAGCATATCTTGCTCATCAGGAAGGACGAATTTTATAACGAGTTTCTAAAATCTGATTTAAATGATCGCTATGATCGGTAATTGCGATTTCTTTCATTCCCAGTTTTCAAGCGTATTGGACGATTTCTTCTACAGTAGCAGAACCATCAGAAAGGACACTGTGTATGTGGTAGTCGGAGAAAGGATCGTTTATGATCATCGTAGCTTGTGAGAAAAATCTAAAGGTTGGATAGGAAGAGTAGTAGTGATTTTGGATTATAAGTCAATCAGGATTTTTTATGAGTGAGGTTCTTTTCTCGCTTTGAAGGTGATAAAGTTTCTTCAGAGTATTCTCCTCTAAAATGATAGAGAGCATTTCTATCCTCTGTAAATAGAGAGAAAAATCAGACTGAAATCTTTTGATTTGAATGTTATTTTTGATTCAGTGTTTTTTCTCTTGATTTTTTATAAGTTATGTATATATAACATATTGTTATATATTTATAACTTTTATTTTCTTCTAGACTGTCATGAAAAAAGCGCAAACTTTGAGATTTCTGGGCATCTTTTGGGGATCCTGGGGGTTATGAATCGGAGCTGATCTCTACAGGCAACTTCCTTTTGAGCCATTAACTCCTGCGTATGGATTTTTGATCCTTTTGATGCTTCTGCTCTTGATCGGCGCAGGAATCAGTCTGTATTATGCCCAAAAAATCAAAAAACAGGAAGGGGCAGTTGAGACTGATGAGATGTTTAAGAAAATTGGGTATACCGCTTTGGCGGCTTCGTTTCAACTTTTGATGGGGATTATTGTGCTCCTTCTTTTGGTTAATGATGTTTCGCCCTTCCTCGAAAAGTTGGGAGCGAGAGGGGTATTGATGATGGTAGCGTTGATCGGGACTGCTTTGGTGCTTTCTTGCTCTTGGTATTATACTCGTCATCCGGATAAAACCTGACTTTAATCTTTATTCCCTTTCAAAATGCAGAATAGAATCAAAGAACTAAGGGCAAAGCATCATCTCACTCAGCAACAGCTCGCCGAAAAAGTTGGCGTTAGGAGAGAGACAATCGTTTTCCTTGAAATGGGAAAATATAATCCTTCACTGAAATTAGCATGGCAGATCGCACAGCTTTTTCAATGTCCGATTGAAGAAGTTTTTCTCTTTGATGGAGAACGTGCAGCATAAAGCTATTCTTCTGGTTTTTTTTGATCAAGTGCTAAAGAGACGTAAGATCAGATTCCTTTCATATCACAAAATACTAAGATGACAAGCCTATTTCATCTTTAAAAGAAAAAAATCTGAAACTTTTCGGAGGTTTTCTGTATACTTAAGGATGTTTACTTTTTGCACTATTTGCAATGAAAAAAGGATCGCTTTCTTTGTTTGGATACCTCTTTTGAGGCTTGCTTTTCGTCGTTTGGATCGGATTTCTTTTCTTTGACGCTAAGCGTGATGAAGATGCTGGGGTTTCTCAGACTACTGTCCTAGAATCCTGATCAGAAACGGTAGTTCCTAAGCCAAATGGAGAAGAGGAAGAAGAAGTGCTTTCCTATTCAGGTTTCCTCACAAATGAAAAAACTGCGAGTCTCGATGCTCTCCAAACTTTAGGTAATATAAGAGAGAAGATTGAGCTTTTGGAAAAGGTCTTTGAACAGCAAGCGACCCCAAAGATTGCGAATCTGCTTCTTCAGGCTTATGTGCTTGATAATCAGTTTGAGAAGGCAAAAAAATTTTATAGTTCATTAGCTTCTGATTTTCAAAATACCTTGCCTAAGGAAATGGCGTTTACTATCTGAATTAATGCCTTTTCTCAAAATTCGGATGCTGAGTATAAGGCGCTCAAGGCACTTCTTGAGTCTTTACACCAGCAGAAGATATTCTCAGACCTTGAGTATAAATATTATACTATTGTTTTTGCTCTCGCTGAAGGGAAGTATGAAGAGGCTAAAAGCTGACTGAGTGCTTTAGAAAAAGGTGAATACCAAGGGTTTGCCACAGCAATCCGTTCTGTCTTTAAGCAATACGAGGATCTCAAAGATGTGCCTGCTTATTATCAAGATGGATTGATTGCACATCAGCTTATGAACCAAGGATTCTTCGCTCTTGCCAAGAAAATTGTTCTTCCGATTGTTAATGAACATCCAGACTATATTCTGCCTTATCAGATTCTTGCCAATACCGATTTTTCTATGGGGAAACGAGCTTCTGCAGTGAGTTATTTTTCTCAGCTTCTCAAGCTTGATCCGCAGGAGAAGAATCTCTATCTTTATATGTTGGGAGTATGTTATTATCAGATTGGGAAGTATAGTGATGCTGTGCTTTATCTTGCCCAGATCTCTGATTCAAGGATTCTTCTGGACTCTGATAGGTATTTGATTCTGAGTTATCTTGCTCTTGGAGAAAAGGATAGAGTTATCGCTTGATGGAAAAGGCTTCTGGGGTATACTGCTATTAAAGAGTCAGATTTCTATTCTTTTTTTGAAGAGATGCTCCGAAAACCTTACCGTAGAGGGGAAACTCAAGAGCGTTCAAGTGAATTGAGAAGCTTGACAGAAACCTATCTCAAGGCTTGCAAACAAAAGCTTTCAGGAGAGCACTTGGAGATCTGTCACTATGGAGAACTGGGAAATAAAGCTCAGCGTCATGCGATTACTGATGAAGATGAGCCGTCAATTAACCGTTTCGCTAGGAGAACGCCTAAACCTGAATTTTTCCAACTCTTAGGAGAACTGGCATTAGAACAGAATGAAAGTAAGAAGGCGACGACTGCTTTTATGAAGGCATTAGGACTTGCCTCTGATACTCAAGAAAAACTGCATCTGAAAAAACTGATTTTACAAGCAAATGACCTAGAAGAATAGTCGTTTCCTCGCCCATAACGTTTATTTCAGATTTTAGGTTGTATTTGAGGAGCATTTATCTATCTTTACGCTTGAAAATTTATTTCTAGTGGGCTATGAGAGGAATCGTTAAAAGTAGGCCTTTCGTCAAAAGGACACGTAGAGGCCTTTCTTCAGGTGGAACACTTCCCTTTGTTAGGGGGAGAAGAAGGGGCAAGGATCAGCCTTCCTTTTTTTCTTCTTTCTTTAAAAAATGGGGACTTATCGGCCTTATTATACTACTTACCTCCGTTTTGATTGTTTTTCTCCTTAGAGGAACCCGATATAATCCTACCTATCTCATAAAAACCATCGAATATAGCCAAGAAACTAAAGCAAAATATTGAAATACTGAATTATTCGTCCTTGCATCCAAATTCCTCAGAGGAAAGTATTATAATACACTTAGGATTGGAGGTGATGGGAAGCTTTTGGAACGAGTAAGGCAAGAATATCCCTTTACTAAATCAGCTCATATTACTTTCCTCGGTAATCAAAAGGTCAAAGTTGATTTTGATTTTTACAGTCCGGAGTTTCTTGTGAAACTCTGAGAAAAGAAGTTTGGAGTCTGGGGTAATAATAATGCTGAAGAGCTCAATCCTTCTCGATCATTAGGAAAAACAGGCTTTGTTATTGATACGCCATGATATCTTAGTGGAACATCTTCACTTTCAGGATTTTTCTATGAAGTAGATTTTACTCGATACAAAAATAATCTTCCAAAGATTCAGGAAGCTTTTCCAAGAATGAAGCGTTTTGTGTATCTGGCTTGATCTCAAAACTTTGTCGTTTTTGAGGAAAATAAGATGATTTTCCTTTTTAAAGAAGAATTGGACCATCAGCTTCAGAAATTTGCTCGGCTCAAAGGCTACTACAGTGGTTATAATTCGCTTACTACTGTTGATTTGGGAAGTTTGACTCAAGATAAAGTGATTGTAGGAAGGTAATTTTTTGTGTCTATGTCATGAGTTAATAAAAAAATTGGTGCTGATAACAGGATCAAGACCAATTTTAGTGTATTAGTATGATTATTTCTCTTACTATATTTTGTTTTTTTTGAATATTTATTTTTTCTATTTTTTTAGTTTAGCTGATATTTATATTGTTGGTTCTGGTCTTTCTGGAGCCCTAGTTGATGCCTCTGGAGCTGGAGTTGGTATTTCTTCAGTTACCTGTGGAGCTACATCTCTTTCTCCTGGGACTGTTTCTGGTCTAGGTTCAGTTGTTTCTGGAGCTTGAGTTGGCATCTCTGGCGCTCTGGTTGATGCCTCTGGAGCTGGAGTTGGTATTTCTTGAGTTACTTGTGGAGCTACATCTCTTTCTCCTGGAACTGTTTCTGGTCTAGGTTCAGTTGTTTCTGGCGCTGGAGTTGGCATCTCTGGTGCTCTGGTTGACGCTTCTTGAGCTGGAGTTGGCATCTCTGGCGCTCTGGTTGATGCTTCTGGCGCTGCTGGAGTTGGTATTTCTTGAGTTACTTGTGGAGCTGCATCTCTTTCGCCTGGGACTGTTTTTGGGCTAGGTTCAGACGATTCTGGAGCTGGAGTTGGTATTTCTTGAGTTACTTGTGGAGCTACATCTCTTTCGCCTGGAACTGTTTTTGGGCTAGGTTCAGATGCTTCTGGAGCTGGAGTTGGCATTTCTTGAGTTACCTGTGGAGCTACATCTCTTTCTCCTGGGACTGTTTCTGGTCTAGGTTCAGTTGTTTCTGGAGCTTGAGTTGGCATCTCTGGCGCTCTGGTTGATGCTTCTGGCGCTGCTGGAGTTGGTATTTCTTGAGTTACTTGTGGAGCTACATTTCTTTCTCCTGGAACTGTTTCTGGTCTAGGTTCAGTTGTTTCTGGAGCTTGAGTTGGCATCTCTGGCGCTCTGGTTGATGCTTCTGGCGCTGCTGGAGTTGGTATTTCTTGAGTTACTTGTGGAGTTGCATCTCTTTCGCCTGGAACTGTTTTTGGGCTAGGTTCAGACGATTCTGGAGCTGGAGTTGGTATTTCTTGAGTTACTTGTCGAGCTACATCTCTTTCTCCTGGGACTGTTTTTGGGCTAGGTTCAGACGATTCTGGAGCTGGAGTTGGTATTTCTTGAGTTACTTGTGGAGCTACATCTCTTTCGCCTGGAACTGTTTTTGGGCTAGGTTCAGATACTTCTGGAGCTGGAGTTGGCATTTCTTGAGTTACTTGTGGAGCTACATCTCTTTCTCCTGGGACTGTTTTTGGGCTAGGTTCAGTCATTTCTGGGGCTGTGATTGGTATTTCTGGCGCTCTAGTTGACGCTTCTTGAGCTTGAATTGGTCTTTCTGGCGCTCTGGTTGTTACCTCTGAACTTCAAATTTTACTCTTTGGCTCTGCTAAAATTACTTTTTTTAGATCAGAAAGCTCTTTTGATGTTTTTTCAAAAATTTCTTTTTTTTCAGAAGTCAGTTGAGATCCCTCTAATTTTCAACTTTTGCTTTCAGTAGAGGTGTTGAATTTTTCCATGGTATCATAAAATAGAAATAAAAGTTATGATAGAAGTATAACCAATATGTTTTGTTTTGTCAAATTTTTATTGACATAATAAAAATCTAGTTCTTGAAAATCAGACTTAGAAAGTTATGCTAGTGATGTGATGAAAAATTTTACTTTTGCCAAATTTGTATGAATAAAAAAGATTTTCCTCTTTTGATAAAGTATCCGGAAATTACCTATCTTGATAATGCTGCTACTACCCAGAAACCTGCCCAAGTTATTCATGGTGTTTCACAGTTTGTGGAAAATGAATATGCGAATATTCATAGAGGGCTCTATGCACTTTCTGAAGCTTCAGAGCTTCACTATTATCAGAGTAAAAAGCTGGTTGCAGAGCTTATTCATTGTCTGCCCAAAGAGATTATTTATACTGCGAATGCGACTGCTTCGATCAATCTGTTGGCGCAAAGTCTAGCAAATTCTGGTTTTCTTGGAAAATGAGATGCTGTGCTGGTAAGTATTTGGGACCATCATGCGAATATTCTCCCATGGATGGCACTTTCCAAGCATTTTGGGTTTACTGTTAAATTCTTTGGGTTGACAGGGGATTTTGAAATTGATTTTGAAGATTTTCATCAGAAATATACCAAAGAGGTAAAAGTAGTGGCTTGTAGTCAGATTTCAAATGTGACGGGTGGAATTTATGATATGAAAAAGATTTGAGCTTCTCTCCGTAGTGAAACGTTTTTTGTTGTAGATGCTTCACAGTCTGCTCCAAATATGCTTGTAGATATGCAAGATATCGGTTGTGATGCCTTGATCTTCACCTGACATAAGATGATGGCGTATACAGGAATCTGAATTCTTGCTCTTAAACAGCAGTGGATTAAGGAGCTTCAACCGCTGAGCGTCTGAGGTGGCACGATAAAAGATGTTTCCTGTGATGGATTTGAACTCCAAACGACAAATGAAAAGTTTGAAGCCTGAACTCCTAATATTATTGGTGCAGTATCGCTAGAATATGCTCTTGAATACCTAAAATCTCTTTCTCCAGAGCAAACCTTCTCCGCCACTATGCAAAAGATTGCTCATCATGAAAAAGATCTTGCTACCTATGCACTCAAAAGATTTTGAGCATTTTGAAATCAGGTGCAGATTATTGGTCCTCAATCAGAAGAAAGAGTTGCTCTCTTTTCCTTTATCGTTAAAGGGAAAGAAAACTTTAATCAGGTTTGAGAGTTTTTTGCTGAAAGAAATATCTGTATCCGTTGTGGTGGGCATTGTGCCTATCCTTTGCATAAGGCATTTTGACTTGGAGGTACCTGTAGAATGAGTGCCTATCTCTATAATGATACCTCTGATTTGGATAAGTTTTTTGAGACCTTGGAGGAATTTATAAACAATTAAAGGAATAGGGAAAAGTTTTTTCCTTTCTATTACGTGGGAAAAATATATACTTGTTCTGAGTATCATTTTGTAATGTTTTATTGAAAAAAACTCTAATGTCCTGACATAAACTCGTGATAAAAGATGCTTTACGACAGCTTTTTGGAAGAATTGTAAGTGCTTTATTTGGTTTTGTGATTACCAAAATTATTGCTTCTTATCTTGGACCTCTGAGGTATGGAGATTATGGGACGATTTTCAGATTTTTTGCCTGGTGGACGGCTCTTGTGGATTTTGGAATCTATGTGATTGCAGTCAAACAGCTTGGGCAGATCAAAAACACGACTTATGGTGAGGGAGAAAATATTCCAAAACCTGACGCTGATTCTGAGTTGGCTATGACTTACGGAAAGTTTGTTGGAATGAGGGCATTTCTTATTTTTATTATCTATACCTTGGCAATCGTTGTTGCGTATTTGATCCCTGCATATACGAGTAATCCGTTTATTGTGCGAGGCTTGCCGCTTGCGATGTGTTATTCTGCGAGTAATATGTTTGCTGGGATTCAGCAGCTTCCTCTTCAAATCTTCCGAAAGATGAAAAGACTGACCTGGTCGCTGATTATTGCGAGGTTTTCTCAGCTTTTCGTCTTGGTTCCCGTAGTGTTTCTTTTCTTCAAAAATATGAATTTTGAAGAGAGCGGAAATCCTGATAGTATGGCGATTTTGGCTTTTACTTTAGTGATTTTTTCTGTGGTGGCGAGTTCTATTGGTCAAAATGTGGAAATTCATCTAAGGACGAAAGATTTACTTCCATTGAAGATCAGATTTGACTTTTCTTTTATCAAAAATCTGCTTTCCAAAAACCGAAAATACGGCTTTTCCTACTTTTTTAGTAGTTTTCATACCTTATTGGTGCTGATGTTTCTTGGGCGGTTTTATCCGACGGCAAGCGGATTTAAATATGCAGGAATTTGGGCTTTAGCACTTTCTTTGATTGAGATCCTTTTGATTGTTCCTTCTTCTCTGGGAAATAGCTTGCTTCACAAAATCCCGCATTATTCTGCTGAAAACAAAAAGAAATCTCTCTGAAATCTGATGCTTTTGGTGGTTTGGATTGGGGTTGTGATTGCGATGAACTTTTGGATTTTTGCAGATGATATTATTGTGTTTGTTTCTAGTCAAAAGTTTCTTGGAACCTGGGAAAACTTCACTTCCTGGGGATCTAATCAGATTTTGCCTTTTTTGGGTATTGTTTTGGTTTTGAGCTTTATCAAGCAGGTTTATAATTATCTTTTTGTTGCTCTTGAAAAGCAGAATCTGCTCTTTGAGATCAATTTGGTCGGAGTGCTGATTGGAGCAGGAGTTGGAGTGCGACTTATTCCTACTTATGGTTTGATGTGAGGAGTAATTACCCAGATTTTGATTGAATTGATCTTTACTTTGGGAGCGATAGGGCTGGGCATCAGAAAACAGCTTACTCCACAATTACCAAAAAAAGTCTGAATTCAACTTTTGGGTATTTTGGTGTTTTTTGCTTTGATTGGAGTAGGAATTCATTATTATTTCTATGTGAGGGGAGTAAGTATGGTTCGTTTTTTCTTGGTGGCTGGAATCTTCAACCTTCTCCTTGTTGCTGCGAGTTTAAAAGCTATGAAAAAGGTCGCGAAAGGATTGACGAATGATTAGCATTTTTATCAATACTGATGTCTGCATATTCTGATGTTTAAAAAGATTTTACAAGCTTCAGCACAATCCTTGCTTTCTAGGCCAAAAATTATGAGGTTGGCTTTTTTTACCTTGGTTTGTTTTTCTATTGTTCGTTTGTATTATCTTATCTATTATTTTAATACTATTCTTATATGGAAATATGAGTCAGGAGTTCAGATTTCTGATGCTTTGATGTACTTTATCAATACGTTAAATGAGCACCATGCCATTGGTACCATTATCGTTGCAATCATTATTATTATTGTAGGTTATCTCTGGCTTTATCCGATTGGGCAGGCTTCTGTGATTTTTGCGCTTCAAGAACCTGAGAAATCTAATTTTTCTGCTTTTTCCAAAGGATTAGGGAGATTTTTTCCGATGCTAGAATATTGAGGACTTTCAGTGCCTTTTGGACTATTTACGTTTTCTACAGTAGTCTTGAGGCTCTATCTTATTGATGTGTTGGGGAATGTCGTGATAGATATTCTGATTGGAATCCGAGGAATTATGGTTTTGTTTGCCAGTATTTTCTGGTCTTATGCGAAGGTAATCATTATCTTGGAAGGTGTTCAGGTATTTGATGCGATAAAAAAAAGTACGAAGCTTGCTTTTCTTAATCTCGGACTTTCAATTAAATTAATGTTTGTGGAATTTCTCCTGCTTTTTAGGTTTTTGATTACTTGAGCTCTGATTGTAGGGCTTCCTCTAGGGCTTGTTTATCTATCTGTCTGGCTTAATATCTTGGATAATCGTTTTGTTGAGTGATTGATTCGAGTAATTGCAGGGATTCTGTTGCTTATTCTTTCCTATATAAATTGTATTGTAGAAACTTTTTTCTTAACCTATTGGTATCAAGCTTATCAAATGATAAGCAAAAAAGAGGAGTAAAGTCTGAATTCATTCTCCTCTTTTTTTGGTAAATTACCTGGTTTATTTAAATTGCAAATTAGCAGGCAAAGTTTTTTTGTATACTTCCCAGTCTTCAGTTGGAACTAAATTTTGCAGATGTATAGCCCAATTTTTTAGCACTTCTAACTGCTGAATTTTAGCTGGAGGTAGGTGATATTTATTGAGTCCTTGTTGCAGTTTTTTAAGCTCTGATAAAAAGTTGCTAGAAGTAGGAGTATCGGTCTTTATCTGGATTGAGTAAGCATCTCCAATTGATTTTAATCTCCAAAGAAGGGTTCTATATGCTCCTTGGATAGCTTCTGAATTTTCTCCTAATTTTGCAGTATATAAGAGCTGGATGTCTCAGAGAATAAAGTTTGGATTGAGTGCAGCTGTGATTTGACTCAATTGAGTTTCTGTTGGAATAGACTTCTCATCTGACTGATAAGCTAGATCTGAGGTAATTGATTCCTGATTTCCAGAACTTACCTCGGTATGATTACTTGCCAAAAAGCTCGTATCTCCAGAAAGGATAGATGGTTCTTCAGGAGAAGGAATTCCACCAGATAAAAGTTGCTGTCCTGTGGTATTTTGAGCAAGTGTCACAGTATGTGTTAGGTTGTTTTTGGTACTTAATACTTCTTCGAGTGTTTCAATATCTGTGATCTTTGCGATATCATTATCTTGAACTGTCAATAATTTTGCAGTTTCAAGTTTTGTAGGAGCTTCTTCAGTCTTTGTCTTTTTATTTTTGATGAGAAGTGGTGCTCCTGCATTTTTGACCTGAGTGGTGTTGTTGACTTTTGTGAGTTCTAATGCTATTTCTTCATCCTTTGTCATGGAAATTGCCATATCTTCTGTCTCTACCTCTAGTGCATCGACATTACGATTCCCATCTAACTTTAGAAAATTACCTTCAAGTAGAGAAAGACGATATTTCCCTTCAGAAATGTAAGTAATTATAAATTTAGCAGGTCATAGAATTTCTGCTTTAATATGCTCATTGATATTAAATATGATTTTGGCGTTTTCTTTGAGGATAATAACATCTCCATCAAAGAGGACAGAGTTTTTAAATTGTTTACCTTCTTTTTCGATGATATATTCTCCGTTAATCTCCAAAATTTCGGCAACCTGTGCGGCACTTACAGATCAAAGTCAAAAGAACCCTTTTTGTGTAAAGAAGGCTCTGTAATCAACAAGTCTTGTGTCTTCCCAGAAAAAAGTACCAAAACAAGCAAAAAATAGTACAAAAGAAAGGAAGGCATATACTCTTTTTTTTGTCAGAATTTTAGATCTTTTGAGTGAAGGAGAAAATCCTGATCTCTGGTAGCAGATCTTTTCATAGAGTGCAAGCTTCCTATTATCTGGAAGATATTGTCTTGATTGCTGTGTAAAATAGTTATGTAAATTCATTTTTGTGGTTGCTATAGAAGATATGAAATCAGGATGAACCAGTAGTCTCTCCGATTCAGTTATTGATACGAATAATCTCGGTTTTTGTGACAAAAAATTACCGATTTTTGCTTCTCTTTTTGATATGATGAAAAGATCGAATGATGTCCTATTGTCTGTTATTTAATTTTAGTTGCAAAAAAGCCGAGTATTAACAGTAATACTCGGCTAATAGGGATATTCTCAGAATTTTCGCTTGTTTAAAGCTTTAACTCTGGGAGGGGTGGGATTCGAACCCACGACCTTATCCTTAAGAGGGATCTGCTCTACCAACTAAGCTACACTCCCATACGCTGTCTCCAGAATAGATCATCAAGTCATACTCTGTTAGAGGAGACAGCACCATATATAGCATTCTCCTTAGAAAATTCAAGTCTATTTTTGCAATTAAGTGATAATCTAAGTTGAGGAAAGGATATTTTCTTTTGTTCTTAACTTCCTCATCTCGCCAAAAACTGCACAAATCTGATAATAACTAAGGATAGCATTACTACCACAAGCCCGACTATAGAATAGGTAATTCCGTTTTTTGCTTTGCTCTTGAGCGAACTTTCTGCTCCGGAGAAAACGTAGAGAAGACCTGAGACAATTAGAGCGACAGTCACTAGTGTTCCGATAAAAATGACGGGAGCCAATACCGCATCTTGGAGAAAGCCCATTGCAGAGGTGTCTCTGTTTCCTTTTCTGATGTCTGTAGCTTCGTAAGCTTTGAATTTACAGGTTCCATCTAGCATACATTCGGTATTGAGGTCTATTCAAAAATTTTCAGATGCGTTAGTTATTCCCATGAGTAAGGGAGCCAAACAAAAGAATGAAAACCAGATTTTTTTTATCTTTAGAAGCATGGTCTTTGGAAAAATAAATAATGTTATTCTTCTGATTTACCGAAGTGAGAAGATTATTGAGCTCCTGCGCTTCAATTTCCCAAAAGCATAATGAAGCTACCAAAAATAAGCGAAACGGCAGCAATTATAAGGATAATAATCGTAACCCATTTCATAACAGGATTTTTTTTCATACAAAAAAGGTTAATACAAGATAAAACATCAACAGACTACAGTTTTAACTATAAAAATCAAGCTCTTTTTATTTTTCTTCTTACTTTCAGTTGCATTAGAAAAACATTTTCTTATATATTACTCGTTGATCTAGGGGATACGCCTAGTGATAGGTGAGGAAAGTCCCGGCACTCAAAAAACGTTGTCCTCCAACAGAGGCTGGATCTCTCTTGAGCAAACGAAAGTGCCACAGAGATAAAACTATCCCACTAGTATTGGGAGAAAGGTGAAACATAAGGGTTGATTTATAACATCATACCTTTACTCAATCAGATTGGTGATCTGATGAGGGGCAAACCGCAACGTGTGCAAGCTTATCATGTTCGTCATGGTAGTGCAAAGATCGCTATAAGCGATACTAGATAAATGTCCCTAATCATACAGAACCGGGCTTATTGGATCAACACTTTCAAAGGGCCGTAGCTCAGTGGTAGAGCGGGAGTCTCCAAAGCTCCGCGTCGCGGGTTCAATTCCTGCCGGCCCTGAAGTAATGGGGGGTTGGTGTAGTGGTAACACATCGGTCTTCGGAACCGCTGTCGGGGGTTCGATCCCCTCACTCCCTGGTGATTAAAAGTTGCTAAAGCTTGGATCACAAAACATTTACTGTCTAAAAATAAAGACTTAACTTGAAAAAGGAGAGAAGATCCGTATGATCTTGTCCGTAACATTAGAGATGCTGAGGTGATGGAATTGGTATACATGTACGCTTGAGGTGCGTATGCCTTTGGCTTGAGGGTTCGAGTCCCTTCCTCAGCATTTTTTTTATTTTCATAAAAGTCCTGCGGTGTCAATTTTATCAAAATTGTTGAGTTCCAACTTTGTTTTGTAGTCGATTTTTTGTTTAGTATTCTGAATTTTAAATTTTTCTATAGCAATATCAGTTTGGCTGGTCTTCGCAAAGTTTGTCTGGTATTTCTCTGTCGTATCAAAAAAATGATTCTGGATGAACTTTGCTAAAAATGCTACGATATTCAGCTCAGAAACCTTGAAAAATGCGATAAGCACAAAAAAGAGAAAAATCAGAATGGCAAAGAACGTTGCGATAACCTTTGAAGATCAAGATACTTTGGAAAAAACGACGAGCGCAATTGCAATACCTATCGCTAGTATAAAGAGCTGAATTATTGAAATGCTCAATGGACCAATCGAAATATTAAGCCCAGAAAGAAGCCCTTTTTGAATATTTTTGGGGAATACTGCTTTCATCGGCTGATCACAAATAAAATTCTCTACTAAGTATATCTGTCCCCCTCAAAAAAAGCAAGTTTTCGCAATTATTTTACTCTCATAGCAAGATAGGCGAGTCCTAGAGCATCTGCTGCATCATTGTATTCGGGAAGGTCTTGGAGTTTATAGAGCTTCATGGTCATTTTCTGGACGAGAAGTTTTTCTGCTTTTCCGTTGCCGGTAATGTATTTTTTTAGTTCAATAGGAGTGAATTCTTTTATATGGCAGCCCTTTTTCATTGCCATAGTGATGAGAATTGCTCTAATTCCATATACAAATTCTGCGTTATTTTGATTAAATTTTGTGAAGAAAAGCTTTTCCATTGCGACAGTCTGAATTTGAAACTTTGCAAAAAGCTCTTCAAAAAAATTAGCAATCTGAATCATCCTTTCAAATTGGTTTTCTCTCTGAGGAGCGACTTGTTTTTGGAGTAGGATCCCGGAATCAATGATTTTTAAATCATCAGTAATCAATGCGTATCAGAGTTTTCTTACTCAAGGATCTATTCAAAGTATCATTAGTGGTCTGAAGGAGCTAAAGCTGGTGCCATTATTTTATCAAGTTCAATGATCTGGTTCCAAATGCTCATTCATTGAATCTCTGTATTGTGCGTAATGACATAAAATTTCATTTGTCTTTGTTTAACAAATCCATCTAAAAGTTGAGCAACCTTTCCGACGGTTTCTACATCAAGATTATTGACGGTTTCATCGAGAAAGAGCATTTCAGTTTTGAGATAAGAGGAGATTGCGAGCATCCGAACGAGTTTCAAAATTGTTCTTTGTCCTCCACTCAGGCTTTTTACCTCTCTTTCTCCTTTTTCGTCAAGGATTTTGGTTTCTAGTTCGAGTTTTTCGCTTTCCTCTTTTAAGCGGATGTTGAGGATATAGGAGACTACTGTGCTGAGATAAGAATTGATGATTTCACTCAAAATAGGAAGATATTCGCTCAAGACAAAAAGTAATAAATCTCTATTTAATACGGTATACAATACTCATAAAAGTTTTTCTTCTTGTTCTAGGTTTTTGATTTTTACTTGTAAATCATTTCGTTCTCAGAGTAAAACTTCGAGCTGTTGATATTTTGATTGGTAATTTTCAAGTACCTGTAGAATCTCAAGCCATTCGGATTGGGGAGCTTCTTGTAGTGTATTTTTCAAGGTTTGTAATTCTCCAGAGAGCGCGACAATTTCTTTTTCAATTGAGGAGATCTGGAGTATGAGCTGTTCATTTACTGTTAAGAGTCAGATTTTTTCCTGTTGATACTGATCTCTATGTGCGAGGGAGTTTTCTTTTTCTTGGAGCTTTCTTTCAATTTCTTTGAGTGAAGTATTGAGGGTTTCTCGCTGTCCTACGGTAGTTTCAAATGCTTTGTAGTCGAAACTTTGCAAAAACTTTCTCAATAGCTCCCTTTTGTTTTGGTTTATTTCCATCTGGTTGGTCAGGTTTTTTTGGATTTCTTGAGCGGCAGTTGTATTCTCAGATGCTTTGATGGCTTGCTCAAGTTCTTGTTTCTTTTCGCTCAGCTTTAGCATGGCAATTTTCTGAGATAAGGCTTCCTGCTCCTGAATAATCTGGTTTTTTTCTTTTTCTCTTTGCTGAAAATGCTGTTGATTGATCAAACGAACGAAGGGGCAAGGTTTTCCGATCTCTCAGCAATCAAATTTGGCATCTTGATTTGTTTGTAGATCAAATTGTTCCAATCTCTCAGCAAGGGATTTTACTTGTCCTTGGTAAAAAAGCTCCTGTTCCGTCAAAACTTTTAGCTGTTCTTGCAGCTGTGAACGGACTCTTTGCTGATTTTGTATTTCTGCTTCTTTTTTTTGGAGCAGGAGCTCATGTTCCTGCTTGAAATGTATTCCCAATTGAAAAAGCTCTTGGATAAAGTTTCTTGATCAGCAAAGGGAGAAATCGCTTCTTGGCTGGAGACTCAGATATGCTTTTTGTTCGTGGTAAAAGGAGGAAATCAAGCTCAACTCAGGGATCGCTTCTCGTTTTTGTTGCTGGAGGAGGATGCTTTCTCTTTGCTGTTTGTAGCTGGTGATTTCTTCTATCTTAATCTCAGAAAGGAGCTTGTCTAACTTTGAAATCTGATCGTTATTCTGGGTAATGGTTGCCTGATGGGTGAGAATCTGTTTTTTTAGCTTATTTTCTTGCTCTGAACGCACGGAAAATATTGCTTGCTTTTGAGATAACTCAGCTTGCTTTTCAGCGACTGCTTTCTGAAGACGGGGAACGAAACTTTTTAACTGGGAATCGAGAGAAAAGTTCTGAATGCTGAGCTTCTCGCCCATTATTTCAAGTTCTTCAAAAATTTTCCTTTCCTCTTCCAAAAAAGGTTGTGCGGTCGGTAGGGTTTGCAATGTTTTGTAGTGTTGTAGCATTCCCTTCAATAGTGAGAGGAATTTTTGCTCATATGGTGTGCTTTCTTGATAGGATCTTATCTGATATTTTACTGCGGTTCTTCTTTCTCTGATGATTTCTTTGGATTCATCGATTCCGAGGAGTCAAAATACGTGTTTTAGCACCTCTAATCTCTGACTTGGCTGCATTTCAAAGATATTCTCTGCATCTTGGAGGAGAAAAATTGTGCTTTCAAAGACCTCTTGGGGTGGAAGTAAACTCGTGAGCTGCTGCTGTAAATCGGTTTCATTTTTAAAGACGATCTCCTCGGGAATAATGTGATGAGCTCTAAGTTCGTCTTCAATATCAAGGTTTTTTTCGATAAGTGCTCTGGTTTGGAGCATTTCTAGAAGCTGTATTTCTGTACATTCGCAAGAAAAAAGTTGCGAACTGGTCGAATCTTTTGATTTCCCTTGCTTGAGGCGTCTGATAATGAGATACACTTGTCCTTCCGTCTTAAAGAGGAGCTTTACGGTTCCTGTTTTTGACTGGATATTGAGGATATTTCTTCAAGAAGATTTGTAGAGAGCAAAACTTGGACCATCAAAAAACAAAAAACTCTTTCCTGTTCCAATAGGAGCTTTGACTAAAAAGTTTCCCTCCTTAAAAAAGAGGCTCAATTTCTGGTCTTTAAAAGGTCAAATATTCTCATAGGTAATTGCGATGAGGTCAAGCATCTTTTCTCTTCAAGATAAAAAGCTTTCGAGAAGCAAGCATAGCTAATTTCTCTAGATTTACAAGTTCAAGAAGCTTTGAGGCAGATCCATTTTCGAAGAGAAGTAAGAAAAACATTCCTGCTAAAAAAGTTTTTCAAAATCTGAATCCTTCCCCTTATTTTTATTCTGATTTTTGGCTTGTGTTGCGGTTTATGAACGGGAGAGGCAATTTAGGCTTGATTTTTTTTGAAGAATCCTAATACTTCTCACTGGAAGTAGCCCTCTCCGTATTGTGAGAGCTTTTTTAAATTCATACGGTGCTGATGCAGGCTCAGGAAATTGAACAAATCAAAAATATCCTTGCGAATATTGAAGCTAGTCAGAAAAAAATCCCTTATCTTTCGGATCTTGAACAGCATCCGGTTTTTTGACCGATTTTTTCTCAGCTGACTGCTGGAGAGAAACAAGAAGTGGAGGAGGTTATTAGGAGCTATATTTTAGGAAAGGTGGAAAGTATTCAAAAAACTAAAGGTGGGCAGCTTTTTGCCAGGTTTGTGGAATCGCAGTCTGAACTTTTTTGGAAGTTTCGTGAGGCAAATGATCCAAGCTATCAGGGCAAGGCATTTCAGAGCTTAGGTAAAGAAGTCGAGATGGAAATGTTTAAACTTGAGGGGATCCTTACTGAGAAAATGCTCAAACAGGAAAAGGGGCTTGATAAAGTGGTAGATTCTTTTTACAATATCATCTATCTTTTCTTTCCAAGATATAATGAGATTGAATAATTAAGTTTTTCAGAGGATTTCATCAAGGCAGACTCTCCCAGCGGAGTTTGCTTTTTGAAAAAAGAGGAAGAAAGTTCTTCTTCTAGGAAGGAGTCCCAATAGAGAATGCAGTCAGACTTCTTTTCTTTTTCCTTCCTTGCAATCCCAAGAGAAATCTCTATAGTTTCTTGTGAAATCTTGTTTTTTGGTGCCTTATTTGTTTCTATTTTTTGTGTTTTTTGGTGATGTTTCAACTCAAATCTGCTTATCAGCCTGCCGGTGACCAACCTAAAGCAATCGCTCAGATTTCTGAAGCTTTTGATCAAGGGAAAAAGAAAATAACCTTGCTTGGTGCGACAGGAACTGGGAAGACTTTTACGATGGCTAATATTATTCAGAAGGTCCAGCAACCCACTTTGGTGATTTCTCACAATAAAACCCTTGCTGCCCAACTTGCAACAGAATTTAAGGCATTCTTTCCTGATAATGCGGTGCATTATTTCGTGTCTTATTTTGATTACTATCAGCCGGAGAGTTATTTGCCAAGTTCTGGAACCTATATTGAAAAAGAGGCAACGATCAATGACGAAATCGAGATGTATAGGCTCGCAACGATGGCTTCACTTCTGAGTAGAAAGGATGTAATTGTCGTGGCTTCTGCTTCTTCGCTTTACGGGCTCGGACAAAGCAGATTTTTTGAGGAGAATTGTCTTCAGCTCTATGTTGGCAAGAAATACGACTTCAATGAGCTGAAAAGACAGCTCCTTCATATGCAATATAAACCGATTCACGGGAAAATCGAGAAAGGAATGTTTGAGATGAGAGGAGATATTTTGGAGATTTTCTCTTCTACAGAAAGATGCCTGTATCGCTGTTTTTTTGATGAGGAATTTTTGGAAAGGATCGAGATGAGAGACGCCCAGTCCTACGAACTCAAAGCTCCAGTGCAGAAAGCGATGATCCGACCTGCGACGCAATATCTCCAAGATGTTTCAGACCTTGAAACGATTCTCCAGCAGATGGATGCTGAGAAAGAACTCCGTATCAAAGAATTTGAGAAAATGGGGATGAGTATTGAGGCAGAAAGAGTCAAAAAGAAAGTGGAATACGATATTAGAATGATTAGAGAAACCTGATTTGTGAATGGGATCGAGAATTATTCCCTCTACTTTGATCAGAGATTGCCCGGGCAACCACCGAATACGATCTTTGATTATTTTCCTGAGGATTTTTTGATGATTATTGATGAATCGCATATGACGATCCCTCAGCTCAGGGCAATGGCACAAGGGGATAGAGCAAGAAAAAATAATTTGATTCGTTATGGGTTCCGTTTACCCTCTGCAATTGACCATAGACCGTTAAGATTTGAGGAATTAGAAGCTGTCCTCGGCTGGAAACCCTTGGATCAAGTAGCTTTAGATGAGGCTGCCGCTGATTTGGAACTCGTTTCTAGTGCTCATCAGCAAGTCCTTGCAACCCAGACTGAAAAGGAGCAAAAATCAGATTCCAGTCTTTTGAATCGTGAGGCTAGAAAATCCAATCAACACGCCGTTTTGGCGCAGAAGGTAAAAAAGGATGCTAAATCTATCTTCCTTTCTGCGACGCCGGCTGAATATGAGCTGGAGCTTTCCGAGCAGGTCGTGGAACAGATTATTAGACCGACGGGCTTGCTGGATCCGATTACTTTTGTGTATCCTAAGTCTGGCGACTATCAACTTTTGCTGGATTCTCTCCCAAAATTGCTCCAAAAGAAACCGCAACTTTCTACTTTTCTTGAAGAAGAGCCTGAGCAGTCTGCAACGCTTCCAAATTTTGAGGAACTCTTCGGTGATGAGTAACATGATTTGAATTTTTGGTCAAGCTCGGCTTTTTGCTTTTCAGGGACTTTTTGAAGTGGAGGAGTGGAGATCGTAGCAAGAGAGAAAAAATACTGATCTTGAACGCTGCGGTTGCTTAGCTTTTCAAAGGTTATTGATTTTTTGGAGTCTGGGGAATTTTTCTTTTGAGGATTCAAGATTTTTTGGAAGTTGAGGATTTTCGTTTCCTTTCTTAGAGGATTTTTTGGAGTAAATGGTCATTTTGTCTCCAGCTCCAGGGGTTTTTTTTAATAAAATTGTTCAAAAAGTCTTTTCCAAAAACTCTTTTTCTTCTCATTTACTCTTTCCTCTTGCTTCTCATTTACTCTTTCCTCTTGCTTCTCATTCTCTATTTCCTCTTGCTCCTCATCTACTCTTTCCTCTTGCTCCTCATCTACTCTTTCCTCTTGCTCCTCATCTACTCTTTCCTCTTGCTCTTCATTTACTCTTTCCTCTTGCTCCTCATTTATTCTTTCCTCTTGCTCCTCATTTATTCTTTCCTCTTGCTCCTCATCTACTCTTTCCTCTTGCTCCTCATCTACTCTTTCCTCTTGCTCTTCATTTACTCTTTCCTCTTGCTCCTCATTTATTCTTTCCTCTTGCTCCTCATTTATTCTTTCCTCTTGCTCCTCATCTACTCTTTCCTCTTGCTCCTCATCTACTCTTTCCTCTTGCTCTTCATTTACTCTTTCCTCTTGCTCCTCATTTATTCTTTCCTCTTGCTCCTCATTTATTCTTTCCTCTTGCTGAAGATAGCCTATCAAATTCCCTTTTTCATCAATAACAGAATAAGGACTAATTTTCGATTCGTTTCTGATGTTCGCTCATTCTCAGATGATCGCTCCTTTTCAGATGCTCACATCATTTCAGATTCCCGCTTTAGCTCAGATGGTTGCACCTTCTCAGATACTCGTTCGTTCTCAGATATTCGTTCCTTCTCAGATTTTCGCATTATTTCAGATGCTCGCTCTTTTTCAGATTTCCGCTCATTCTCAAATGCTCGCTCATCTTCAGATTTTCACATTATTTCAGATTTCCGTATCATTTCAGATTGTCGCATTCTTTCAGATTTCCACTCATTCTCTGATGTTCACATTCTTTCAGATGTTTGCTCCGTCACAGATTTGCGCATTCTTTCAGATGTTTGCTCCGTCCCAGATTTTCGCTCATTCTAAGATGTTCACTCATTTTCTGATGTTCGTATTAGCTCAAATATGAACATTGCTTTCTAAAACAACCCCCTCCTTAATAATGACATTTGGTCCAATAACACAGTTCTCTGGGACATAAACAGAAGGATGGATCTGTGCTGAAGGATCAACCTTTGCAGAAGGATGGATCTGTGCTGAAGGATCAACCTTTGCAGTAGGATAAATTTTGGTAATTTTAGAACCTCCACTCTCAAGAGTAGCTTTAATAGTTCATCAAGTGGCTTCTTGACCTGAAGATGATATTCAAGACTTATGGATTATCTCATTATTTCAGACAACCTCATAAGAACAGATTTGTTCATTATCTCAGATTATCACTTCTCTTCAGATTCTTGCTTTATCTCAGACTATCACTTCTCTTCAGATTCTTGCTTTATTTCTGATTAGCACGGTTTCTCAGATTGTTGCATTATCCAAGATTATCACTTCTTCTCAGGTTATCGCATCATTTCAGATTTTTACTTTATTTCCGATTTTGGTGTCAGAGAGTATTCTCACTCGATCTCAGATTATCACTCCAGCTCAGATTTCGATATTACGCCAGATTTGTGTATTAGCTCAGATTTTCGTATTATTTCATATTTCCGCATTATTTCATATTTCCACTAGATCTCAGATTATCACTCCAGCTCAGATTTTGTTGTCAGGAAACATTTTCACTAGATCTCAGATTATCACTCCAGCTCAGATTTTGTTATCAGGGGAAATTTTCACTCGAGATCAGATTATCACTCCAGCTCAGATTTCCGTATTAGCTCAGATTTTCGCATCACTTCAGATTTTCGCATTATTTCAGATTTCCACTCATTCTCAGATTTGTATCCTATAGTAGATCGACGCTCTATCTCAGATTTGCGCATTCTTTCAGATTTCCACTCATTCTCTGATGTTCACATTCTTTCAGATGTTTGCTCCGTCACAGATTTGCGCATTCTTTCAGATGTTTGCTCCGTCGCAGATTTCCGCTCATTCTCAGATGTTTGCTCCGTCGCAGATTTCCGCTCATTCTCAGATGTTCGCATTAGCTCAAATATGAACATTTCTTTCTAAAACAGCCCCCTTTGCGATAACGACATTTGGTCCAATAACACAGCCCTCTGGAATAACAACAGAAGGATCAATCTGTGCTGAAGGATCCACCTTTGCAGTAGGATGAATTTTGATAATTTTAAAACCTCCACTCTCAAGAGTAGTTTTAAGATTTCCTGAAATAGCTTCTTGAGTATTCGCTATTGCCTCCTTAGTTCCTCAAGTTTCTGAAGTTTCAGGATTAAGAGTTCATTCCTGATGCTCTGCTCAAGAGGTATATTTGCTCATTCTTATTTTTTACAAGAAAATTAAAAAAAACAAGATAAATATACAAATACAAAACTTGATGTCAAGTAAACTTTTGGAGTAAATGATTGTTTTGTCTCCAGCTCCAGGGTGTTTTTTAGAGTAAATGGTCATTTCCTCTCAAAAACCAACGGTTCTGGCACAAAAAAGTCTATTTCCTCTCAAAAACCGACGGTTCTGGCACAAAAAAGTCTATTTCCTCTCAAAAACCGACGGTTCTTGGGCAAAAAAGTTCATTTCCTCTCAAAAACCAACGGTTCTTGGGCAAAAAAGTCTATTTCCTCTCAAAAACCGATGGTTCTTGGATAAAAAAGTTCATTTTCTCTCAAAAACCGATGATTCTGGAGGAAAAAGAGGGAAAAGAGATGAGGGGTTAGTGCGTTTTGAGAAGGAGGGGAATACTTTCTCCAAAAAATATAGAATACCACAGTATCCTGAAAAATTTAAGTCTAAAAAAAGATGGAAGTCAGATTTCTTTCTTTTTTCTTACTATAAAAAATGGAATTTAGAGATAGGAGAGCTGTGAAGGTTGGAAAAATCTGGTAGGACTACATAATAATGTATTCATCAATGAGATTCGCGTCGCCTGCGGAGATCATCCCGAGGAGCTCCTCGTTTGGATCGCCGTTTTCGGTGATGAAGAGCACGCCAAGCTTTTCGCCATTTTTTTTGCTCTGGGTAAAGAGATCGTTTACCTCAAAAATCGTGGTGTTGCTCGAGATGAAGAGGTAATCTTTTCGTCCACGGTTGAGCTGGATGTGGTCGACTTTCATGAGTTTGAGATTGAGGTAATCATCACTCATAAGGAGGTCTGCGATTCGGTATAAGAGTCTGGATTCACTGAGGATGCCCGCGAAATTTCCTTGGTCGTCGTAGATCGGGATATTGGTATAGTTTTTCCCTTTCATCAAAGGAATGATCTCACTCAGGAGATCAGATTTTTTGGCTGTAAAGACGGGTTTTTGGAAAAATTCGAGGACTTTTGCTGGCTCGGTGATCTTTTGGGCATAGGTTTTGATTTTTTCGATAGCGGAGGAGGTAGGCACGGCATAGGTTTCGCCGTTGGATTTGATTCCATGCGTGATAAAGTTTCTTAATTCACCAAAATTTCTCAGCTGATAGAGATGTTTTCTGACAAAGCTAGAAATGGTATAGTTGCCGTCGGCGATTTTTTTGAGTTTTTCGTTGTAGGGGGAGAAGCCCTCCTCGTTTAGGACTTTATCAAAGTGTTTGTCTATTTCATTGAAAAAGGCTAAAAAGCTGACGATGCCTTCCTCTTGGCTTGGGATGATTTCTGCTACCATATGCGTTCTGTCCTCAAATAAAACGTAGTTAAGTATATTCTTTCTGCCTGAGAAATGCAAATTTTACCAAAGCTTCAAAGTCTGAAAAATTGTCTTGATTTTTCCCCCCCATTTCTGTATAAAACCACACATGTAAAACAACACAACTAAAATATATCAGAATTTATTTCTTTTTTCTATCATGACTCAGTGTGTGAAATGCAAGAGTTCGGAGACGAGAAAGCATGGTTTTGGCAGGAATGCGACGCAGAGGTTTTTTTGCAAGCAATGTCATTCGAGCTTTACGGTCGATGGAGTCAGAGGGACCTATTCTCCTGAGTTTAAGGAGTCAGTCGTGGAATCCTACTGCCACCAGAGGCATACAGCACAGGATGTGACTGAAAAGTATGGAATCTCGACGAGGACGCTGGTTAAATGGAAGAAAGAACATCAAACCCATTGTGATTGTGGGGAAAAATAATATTTAACTTATTCATATCGTGTACTTTATGGAAAGATTGAAAGAAAATCAGGTTTTTTCTTTTTGTTCTCGTGCGAAGAAATTGGCTAGTTATGAACCATCGGAGGAAGTGAATGCGTTTTTTTCAGAGCTATGTCAGTTTGCTAGGGACGAAACAGATTATGAGCTGGATTGCGATCCGATCTTTGTTTCTGATGAAGTGAAAGGGTTACAGGAGATCTGTGCGGTTGCCGAAGGGGAAATGGAGAAGTTTTGGTCAAAGAAGATTCAATTTGCTCAGGATCCGCAGAAGATGCTCAAGAGCTTCATCTATTATCAGAACTATGAGGATTTAACAGGTCTGGAATATGCCTTGCTTGCTCGTCAGATCGGGTTTTTGCAGCAGGTTTTGTTTGTAGGGAGCTGAGCGTTGCCCTTGACTGCGATTATTTTGGCTCAGAAGTGGGGAGTCCGTTCTGTTTTGATAGAGAAAGATGAAGAAGCTGTAGATCTTTCTCGTGGATTGATTGAAGCACTTGGGTTACAGGAGAAAATAGCGGTTGTAGCGTCAGATTTCCTTGATTATAGGTGAAGTGAGACGGAGTATGATGCTGTGATTCTGGCTTCTTTGCTTTTTACCTCTGGAGATGCTGAGAAGCTCGTTGATCATCTGACTCGTTGCGTGAAGTTTAGAGCTTGTCTGGTAAGGACGGCGAGAGGAATGAGGCAACTTTTATACAAAAAGGTAAATGAAAAACTTTTAGAAAAATATATGAAGCTAGAACTTTTGGTTCATCCACAAAATCATATCTTAAATTCGATACTTTTATATTCTCATGTATAATTATTATGTCAGAAGTGAAACTTAATGCGCATTTACCTTTGCGTGAACAAGAAAAACAAAACACTATCGTAAATAAAATTTCTGAAAATTCAGAGGCTACAGGGAATGAAGTTTTTTTGATTTTATGATTTCTTGGGGCTGGGAAAACGACTCTGCTCAGGGATTTAGCTGGATATTTGGCAAATCAGTGAGAGCAATTTAAAGTTATCGTAAATGATATTGGAAAATATAATATTGATGCTGAGAGATTGAAAGATTTTTCTCCTGTCGCTTTGACGGATTGATGTATTTGTTGTCAGGATTTAAGCTGATTGAAAAGTGCTTTGGAGGATTTGAAAGGTAGTAATGAAAAAATTTTGATTGAACCTTCTGGAATCGCTGATGGAAAAGCTATTAAGAGAGTTATTGAGGAGTTAGGCATGCAGGGGAGAGTAATTACCTTGGTGAATGCTCAGTCTTTCCCAACCATGTCGAAAGCTGAAGAACAGATTGCTTCTACACAGCTTTCTTTGGCGAATGTTATTGCGCTTACGCATCTTCCTGCTGATAAAGAAAAGAATCAAGAATTAAGAGCTCAGTTGTCTCAGCAATATCCTCATCAGCCGGTTATTGAAGCGCCTTATACAACCAATAGTGAGGATCTTATGAAAGATGATAGTTCGTATGGAGCTATTGTAAATCTTATGAGAAATCATTCTCACAATAGTCATGCTGAGCATGAACACGAGCATGATCACCACGAACACGAGCATGGTCACCATGAACACGAGCATGATCACCACGAACACGAGCATGATCATCACGAACACGAGCATGATCATCATGAACACGAGCATGATCATCATGAACACGAGCATGATCATCATGAACATGGGCACCATCATCATGAGCATTCAAAATTTTTCACAAAGAGTATTGCTTTGTCTGAAGAGACTTCATTAGACCAGTTGAAAGACCTTATTCATCAGCTTGGGCAAGATGCGCTTCGTATAAAGTGAGTTATTAAATACTGATCTCAGGCTTTTGAATTTGACTATGTGAGAGGAGGTGAGTTAGAAATCTGAAATATGACGACACTTCAGCCGCATCTTAATGTTATTACTCAACGTTCTTTATCTCCAGAATTGATAGCTATGATTTCTTCGGGTTCAACTTCTTCTGAAAAACTTTCTTATGTACCAGAAAAGAAAATTTACTCTCCAATAGAGGTTTCTGCTGCGGTTTCTGTTCTTTTGAATCAATATAAGGACTATATGAATATGTATAATGAAAAATTAGCTTTAGAGACTGAATATGCCAAAAATCAGGATTCTAAATTGGCTCAAGAGATTCAGCAGCTTGATATTGCTCTGGACAAGCTTTGAGATGATATGAAGTTTGATAATCCATTGATTTGGATTGGCTATAAGAGGCTTGCATATGCAAATAATCCTGAAAAAAAGGTAGAAACTATTTGAGAACTCAAGAAGCATTGTGAAAGAAAAACGGATATTTGCCACAAAAGATTAGATTTTTTGAATGAGCATCTCAAGTCAGATTTTTGAATTGATCTTTTTGATGAAAATCAGGTAAAAGCTGATCAGTCTTTGGTTGACTTTATTAAGGAAAATGAAGCAATTAAAAAACTGAGTCAAGATGAGCAATTTATGACTCAGTGGCTTCAAAATGAGTATTTTCAGATTGATGGAAAAGTTGCAAAATGGCAGAACTATCAGTCTGTGCACTAATACGCTTCTACAAAGATATTTTTATATCCTCTTGCTCTTAAGTTTGTGAGGAATTCTTTGACCATAAGTGGTACTCAGCAGATATAAAACTCTGTTTCTGCTGGGAAATCAATATGATCAATATTGATTCTTCCGTATTCAAATCCCTCAACCTCTTCTCTAGAAATACTAATATGTGTTTCAGGAATGTTGAGGTTTTTGATTTCTTCTCCATAAAAAAGATCTTTTGCATAGGAAACTGAAAAGAAAAATCGTTTCTGGGATCAGCTTTTATCTTCTTTTGCCATACGATAGCAAGGTGCTAGTCCGCTTCCTGTTGAGAGGAAAACTTTGGGATTTGTCGTATCTTTAAGCAAAAAATGACCGTTTGGACCTTCAAGTCAGAATTTTGTCTGTTCATTTGCTATCCTTAAACGCTCAGAACCTAATCCTCAAGGAATAAGTTTTATCAAAAAGGTAAAAATACTGAAATCACCTTCCTGTTCCTCATTTGCGATAGAGTAAGCTCTCTTGAGCGGTGTTTCGCTCTCTAAATAATGGATAAAAAGCCGTTGTCCAGGCTGAGTTGCAAGTTTTTCTGTTTTTAGTTTGAGTTCTATTACTTGGTCATTGAGGAATGTTTTGCTGATAAGTGTGGCTTTTTGCATGGGTAAGGTTTATGAGTAAAATCATTATTAAGTGTATGATTTTTGGGCTATTTTGCAAGTTTTTGCACTAAGAGAAAAATCCGAACTCATCTTATAGTCCGGATTTTTTGATTGATTCAATACCTAGGATGGAGAGGGCAGCTCTGTCATTTTTGACGCAGAAACTTTCGATAGAGCTGTTCAAACTTTGTCCTATACACGGTATATTCTGGATAAAGATAATAATATTTTCTACAGAATACAAGAAATTTCTCCCCCTCATCAAAGGAGAGCCTCTCTATCCATTCGTAGAGGGTTTTTTTCTCAGGTGGTCTACGTTTTGTTTTTATATTCATTATTTTTTTATGTTTTGTGTCTTTATAATATATTTATTTTTCTAAAAGTCAATACTATTTTTTGTATATATTGAAAAAGTTTTGCTTGATTTGCTCTCGGAAAAGATCTTCTTTTTGTCCTACTAATCATGCTATAAAAGATCAAATCTGAATCACCCTTTCAGGCATGTTTAATTTTCCGCGAAATTCTTTTGGAGCGAGGTAAGGAGCGTCGGTTTCTATGAGAAGACGATCTTTAGGAATGCAAAAGATGCTTTCCCTGAGTGTTTCTGCACGAGGATAACTTACATTTCCACAGATACCAATGTAAAGGTTAGGGAAAAGCTCTAGAAGTTCTTTGAGTTCTGCAGGTCCGTATCCTCGACAATGGAAATAGATTGTCAGATTTTGGTAATTTTTTAAGATTTTAACAGTTACTTCAAAAGCGTCTCTTGAGTGGATTACAATGGGTAATGCTAGCTCTCTTGCGAGTTCACACTGAGCTTCAAAGACTTTTTGCTGGAGAGAAAGCGTTTTCCCTTCAGTATCATAATGAAGATCAATCCCGCATTCTCCAATCGCTACTACCTCTTCCTGATGATCTATAATCTGATTTTTGAGCTCTTTGATTTTTTCATCAAGAATTCCAGTAAGTGGCACTACATCACAGGGATGAAACCCAAGGCAGCATTTTACTCGGCAATCTGGGAAAAGCTTTTTGGCTTCTTGGGCAATTAAGAGCCCCCTTTCATTGTAGTTTTGGTTTGCTCAGGCATTAACTAGTCCTTTCCCTCACTTCTCGATGAAAGATTGGAGTAATGTTTTCCAGTTTGGGAAAAGTTCTTCATGATTGAGGTGAGTATGTGAGTCGTAAAATTCCATAGCTACGAGGAAAATAAAATATTAGCTTGACTTTTTGTGAAGTTTTGATATAGATGAGTGTGTTATAATAAAATTAGATCTATGAAAAGACAAAAAAAATCAAATGATGAGCATAATCACACTCGGTTTACTGTGATGTTGCTTTTTGTGCTACTGTTAACAGCTATGCTTCTCCATTTTCTTATTAGAATCTGGAAATAAAATCTGCCTAGTTTAGGCAGATTTTTTCGATGTGGAAAGGTAGTCTAGGTATTCTAAGAGGTCAGGATGCATATCGGGGGTTATTTCTGCATTGGCTGCTTTTTCACACTCTTCTTCTCAATACTCTTTGGTTGTAGATTGGTCGCCTTTTCGTTTTGCTACAGCCTCACAGAAGCTGAATCCTGGAGTAGATGCTTTTTTTTCATAGATAGGAGAGAAGGTTGGTTGGATATAGAGGGTATCTCCTTTAATGGTAGTTGATCAAAGTTTGAAATTCAGATTTTTAAGCTGGTGTTCTTGCTGAATAGGAAGATCAAAAAAAAGATAGCTGTCTCTTTCTTCTCCGTTATTGCTTCCGATTTTAGTCCCTGAAGTGTTACCTGCAAGATCCTGTAAGCTATAGCCTCCTGCATAATAGTATTTTGGTAGATACTCCTTTCCTTCGTATTCGGCAGCAATGATATTTTTCACTGCTCCACTAACATTTTGGCTCCAATTGAGCTGAACATAAGAATCTTTGCTCGGAAAGGTAAAGTAGTCGTTCTCTCCTCGGCTGATACTCAACCATGGCATCATTACTTGTGATTTGAAGCGATACATGGCTCCAGAAAATCAGACTTCTTTCTGGTTTTGTGTCCCTTTTACTTTGTAAAGATGATTCCCAACTTGACGGATAAGTTCCCAATCTTGAGTAATTCTTTCCACCTCTTTGGTATTATTGTCCTCAAGGGCTTTTTTGAGGTCGCTTTTTTGCTGTCCGTAGATGTTTTGCATGGTAGAAAGATAGTGGTCAACGAGTTCTGGATTTCATTTGATGTTCGTATCTAGTTGGGTGATCAATCCAGAGATAATGGCCTGTCTCTTGCCAGTTAGTGATTCATTGGCAAAGCTCAGATTCCAAGCAAAAGCTCCGATTAGAGTGAGGATCAAGAGTTTTTTCATTAAGATTGTGAGGAAAAATAAAAAAAGAATAGTGGTGTTGTATGCTTTTTGGGGTAAAAAGCAACTCAAAATTTATGGAGAAGGCGTCTAATTGTTTTTTTATCTATGAGTGCTTGCAATCGGTTTTTACTTAAAAATAATCCAAACCTCGAAAATAGTAGCATTGTTCATAGAGTGAAAAATATGTTTACATCAGAAAAAATATTTAACTACAAGTGGAGCGAGCAATTCACTATAATGATGTTGCTAAAATGGTGTTTCAGTATAGTATGTTATTCAAGTTTTTTAAACAAAAATCCTGCACTATCGTTAACTCTTGGCTCTTTGGCAGGAATCCCTAGATAAATGTTTTTTCATAAGGTGTTTTTATGCTCTATATCTCCATCTTTGGCGAATACTTCATTTTCCTTAAGGTAGGAAAATCAACTAAAATCTCTTGTAAATTTGAAGTCTTTGGTTATTGGCTGAATTTTTTCTCTAAATTGGTAGATGTTCTGTTGTTTCCTTTCTTTTTTTCTATTATTTGGTGGGAGGTATTGATAATATTCTAAAAAATTCAGAATTTCTTCTTTCCCTCTCTCAAATCACTCTGGATCATCGTGCAGTCCGCATTCGATTCCGTAGGCTTCTTTTTGTTTTCTGGTGCAATATCCTATAATACTTCCTGATTGTTCTAAATTATCGACCAAAATTGTCTCTGTGGTGAATATCTGCTTTGCTTTTTCTCTATTCTGGATATCGCAAATTGCTACAATATCATGACTATTTGAAAAACTATGAATATCTAAAGTAATATCCATTTCTCTAAGTATTGGTTTCAGTTCACCTAATCTTTGATGTTCATAGCTTGTCGATTTATACGCTTGATTAGAAATACGGTTCATGTTATCATCAATGAACCTTCTGTGTTCCTCATAGGCAAGGAGATTCATTGCGATGAGAAAGAGTTTCCCTTGCTGGAGAGGGTGTTTTTTGAGAATTTCCTGTTTTAAGTATGCGAATACCTTTAGTCCTACGGTTTCGTTTCAGTGGGTGATTGCAAGGAGTCCTAGATTTTTTCAGGGGTTTCATGAATCAATGAGCGTGATCCCTGAGATGTTTTTATATTGATTTGTTAAGAATGGATTGGTTATCATTATTTATGATATACAATTAAAAATGCATCTCATTTCTCTGGCTTAAAGTCAGGGAGGAATCGTTTATTTGTAAATTTTACATTCCCTTTATTTCTTATTGCTCATCATACTTCCTTGAAAATTTATTCATTTTTTTGACTTGGATATAGCTAGTGAAATTTTGCTAAAAGTCAATTCTTTTTTTGGAAGAAATTTCCTTCAGGGAAAAAAAGTATTTGTTAATTTTTAGTTATGGAAAATGAAAAAAATCTGAATTTTTTACTCTTAAAAAAAGAATTTTAGGACTTTATAATGCTTGCTTGATTTTTTTACTGTTGTTTACATGTGGAGGCGAGGATTTTGGTGAACAATATGATGCTCTGAGTGAAAAGTACACTGAAAAAGCGAATATTCCCTATGAATGACGAGCGGGAGAGTGTCAGAACGTCAGTTTTCTAAAATCTCATTGCATTTTATTCCGAAATCTGTATATTCTTTCACTGAATCTTATTTTACTTCGTTAAAGAAGCTTTTTATAATGCCAAGAAGAACATCTAAAAACAGAAACATTGAGGTTATTTTGCTTCAGGATAACAAACATCTCGGGGAAAAATATGAAATTATTGGAGTGAAGCCTATCTTTGCTAGGAATGTGCTTTTCCCACAGCAGATTGCTGTGCTTGCAGACAAGGCAAACAGGAATAAATATGAGCAAAAGATGCAGGCGGCTATAGCTGCAAGAGCGAAAAAAGCTAGTGGGCTTGAAGAACTCTTTGCTAAAATGACAGAAAATGGAGGAGTAAAGCTTTTGAGAAAGGCTAATAAAGGAAAAACGCTCTATGCTAAGGTAGGAGAAACTGATATTACTGAGGCAATCAAGGAGGCTTATGGAGTGGAGGTAGAACCTCACTATCTTAAGCTCAAGAAAAAGCTCAATGAAGTAGGAGTTTTTGTGGTTCCTTTTATTTACAAGGATCTTAAGAGAGAGATTTCTGTGACGATTGAGGCTGAAGGTGGAGTTCAAGAAGAAGTTAAGGTAGAAGTTGAGGCAGTGGAAGCAGCTCCAGAAAAGACTAAGGAAGAACTTAAAGCTGAAAGAGAAGCAAAAAGAGCTGCTGAGAAAGCAGATAAGATTGCAAAGCTCAAGGAGAAATATAAATAATCTTTCTTTTCACAAGAAAAGTCTCTCTGCGGAGGGATTTTTTTTATTGATGGAAAAGATTTTGAAGGGGAGAGAGGGAGCTTTTTTTGCCTGAGGCTTTTTGTGATGGAGGGACCTGCTTGTTCTTGATAAGAAAGCTTTTTTGGGATGGTTGAACTGGAGTTTGCTTCTTGTTTGCTGAAATTTTTAGAAAGAGGTGAGAGTGATAGGGCTTGAAAAAGGGGAAGAGTTGGATGAAGGTTGCTTGAAACTGGGTGCTATGAGGTAAATTATTGGCTTGCAAAAAGTTGGGTAAAAGGCTTTGACAATGCTCTCTAAAACGCTACAATCGAGTCGTCTGATTTTTGAGAAGAGGGGAATTTTTGAGAGGAAAAGAGAGATTTGCTTGTGAGTGTGAAATGTCGATGTGCTTTGGATCATCCGCAGTGCAGAAGAGAGTTTGTTTTCAAAACGGAAGGTAAGGAAGTGTTTGTGATGGTGCGTTGGAGGCAAGCTGAATCTTGGAAATTTATCAGACTTTTGAGGCTTTTTTTATTTCTTTCTAAAGAGTTAAGAAAAATTATGAACTACGGAACGATCAAAACCATGGATACGGCGAATGGTGAGGGGCTTAGGGTCTCTCTTTTTGTGTCTGGGTGTCATCATGCTTGTCCGGGGTGTTTTAATCCTGAGGCGTGGAATTTTGCTTTTGGGAAGCCCTTTACACAAGAAACGATTGATGAGATTATCCAAGAGTTGAGTGCTGATTACATCTCGGGATTGTCGCTGCTCGGGGGAGAGCCTTTGGCGCCGGAGAATCAAGAGCAGGTTTGGAATTTGGTTAGGCAAGTTAGAGAGCAGCTTCCGCACAAAACGATATGGTGTTACTCAGGGTTTACTTACGAATTTATCACGGAGGTGATGTTTCCGAATTTACCGTTTACGTATCAGATTTTGTCGCATCTGGATGTGCTGATTGAGGGAAGGTTTGTGCAGCAGCTCGTAGATCTCACGATCAAGTTTAGGGGGTCTAGGAATCAGAGAGTGCTGGACGTTCCGCTTTCTCTGGAAAAGGGGATGCCGATTTGGAGCACTGCGGTCGATGACCAGAAGGCTTATGAGTATTATCCGAGCCCTGAGAAGGAACTCTTTACTGCAAGGATCGAGAATTTCCTTCACAAGGTGACAGTGGAGTAGAAAGTTGGTTGGCAGGGTGGAGAAAGGTTAAGAGGACACTGGTTATTGGAGCGTGCCTTCGAGTTTGAAGACTACGCCATTGGTGATTTTTACTGGGAGGAGGGATCAGATTTTTATGCCTTTGGTCTTTGCGTTTGCTTTGATGGTGATTTGTTTCATATTATCGGTATAGCCGTAAAGGTCGCCGTTGGGACCGATTTCGTTTATCATCATTGTTCTGATTTTCCCGATTTCCTGTTGGTTATTTTCGGTCGAGATTTGGTAGAGGAGTTTGTTGAGGCGGGATCGTCTCTCGTGTTTGACGGAATAAGGGATATCGTCTGGGTATTTTCTATCGGCGAAAGTGCCGGGGCGGTTGGAATAGATTCCCATATAAATCATATCAAAGCGTCCGTATTCGGTCAGTTTTAAGGTTTCTTGGAAATCTTGTTCGGTTTCTCAAGGAAAGCCGAGGATTATATCGGTTGTGATAGAGATTGGTCTGCTAAGGGACCTAATTTTATCTATAAAAGTATAGCACTGCTCTGCGTTATAGCCGCGGAACATTTTTTTCAACACTGGAGTAGAGCCTGACTGGAATGGAATATGGATATGAGGGCAGAGTTTTTGCTCGTTTTGGTGCAGTGCGAGCAGCTCGTCAGAATAGTAGGTCGGGTAAGGGGAAGTGTATCTGAGCCATTCTAAGCCCTCTAGTTTTAAAAGCTCTTTTACAATAGTCAAAAAATCTGGGTGTTTATTGACGATTTGTCCGAGGAGTGAGATTTCTTTGACGCCGTGAGCGAGGTGGGTTTTGGCTTCGTGGATGATTTGCTCTACCGGGAACCATTTCTCCAGTCCACGCGCATATGGCACAATACAATAGGCACAAAACTGGTTGCAGCCGCTTGAGATAGGGATGTAGGCGGTTTGGTCGTGGGCGTTCATACTGGAATTGATGTTTGCTGGGATGATAGTGGAGTATTCATTGGTCAATTCTTGATCATAGTGGATGTTGTAGCCGAGTTTTTGGAGCATAAGAGGGAGGAATCCGGTATCATCTATACGGAAAAAGAGTTCAATATTCGGTCGCTTTTGGGAGAGGGAATGAAAAAGAGGATTAAAAGCATGATTGATCCCTACGATGTTTGCAGTATCGGGTTTTTTGAGGGTATTGATCTCTTCGGAGGTGAGCCCTATGATTGTAGGCTCGTTGGTGAGAACCGCTCCAGAGAAATTTCAGGTTTTGAGCTGGTCTGGAAGTTTGGCAGCAGTGCTGACTTTCTGGTTTCTCATGGTATGCTGGATCATACAGCCGGTGATTCGCACTTTTTTGGTAAGCGGGATTTCCTTGAGTTTACCTGTGACTTTGTCCTCTGACTTTTGGCGGACGGAGCAAGTATCAAAAATAACGATATCTGCCTCGGCGATCGTTTCTACAGGATCAAAACCACAATTTTTGAGAACAGATTTGATTCTGGCGGTGTCGGAATAGTTCATTTGGCAACCCAAGGTAATCACGTGGAATTTCATTCAGTATTTTTTCTAATAAAAGCAACGTGCATCTTATGGATTTGCTCTCAAAAATCAAAAAGAAGGTCATTGGGTGACCTCCTGTTTGTATGTTTGGTTATTCTTGTCTGAGATTACGTTTGGTAAAGAGGACTGTGGCTCAGCCCTTGGAGAATGCTGAGACTCAGATCAAGTGCTTTTCATCATAGAACTCTGGCTGGATTCAGATGACTTCACAATTATTAGGGTTTTGCTCTGGTCTAGTGGAAGCTGGCATACCAGGAACTCCTTTTGTGATGAGGAGACATGATGGGGTTTTCTCATCCTCAAGTCAAAAGGGTTTAATCAAGATCTGAAAAGTATAAGGAATGGGGTTTAGAATGCCTTCATAGCGTGGATTATAGGTAAGGTATTTGAGCCCTGCTTTTCCACTGAGAGCGGAAACTTCAAGTGCGAAGTTTCGATCACTCGCATAGAGGAAGGTTGGCATTGAAACATCGATTCTTCCCTGAGGCACATGAATTTCCGTGTCGTAAGGGATGCTTTCTGTGGTGGCGAGGTTGAAACCTTGTTCTTTTTCAAGGACTTTTTCAACGATGTTATCCTTGATACGTAGAATACTTTCGAAGCCTAGTCTATACATATGGTATTTTAAGTAATTACTTGAAGCGATGAGGATCAGACTTAAGAGTCCAATGATTGCAAGGAGTGTGGTCAAGAGTTTTGTTCGTCTCTTGAGGACGGTTTTGTTGAAGCAGGCTTGGATGAGCTGAGTTTTTGTGGATGTTTTCATTGTGAAGCTTGGTAGAAGATAAAATGATTTTTCGTTTTATAATTGCCTCCCTATTGTGAAAGGTTTTGTTATTTTTTTATGAGCTCTTCGAGGAGAAGATCATTGCTTTTTTTTCTGAGGTTCTCGACTTGGAGTGATTTATTTTTGAGGAATTTTTCTAGATCGAGTCCAAATTCTTCCAGCATGATAGATGTATGATCAGCTCCGATATAGTGGGGCATAATGACGTAATCTGCACCTTTTTCGTAGAGGGTGATCGCCTCGTGAATGTGATTTGCGATACAAATGACGATGAGATCAGCCTTTTTTTCTTTTAAACAGCTTAAAATTGCGAAGTTAGCGTCCACGTTTCTGATCGTTGAGATTACCATTTTGGTGCCTGAAACATTAATGTCATTCAAAAACTCTAAATCGAGAGCATCTCCATAGAGGCAATTGATTTCGTGCGTGTTGAGGTGGTTGATAATAGCAGGATGCTCGTCAATGATTAGAAAAGAATACTTCTTTTTTTGAAAATGCTCATAAAGACTGCTTCCGAGTTTGCCGAAGCCGAAGAGGACGATATCATTTGGGCTTCCAAGAGTTTCCTTTGATTTTCTGGAGATTGAACCCGGGATGTATTTTTGCCGTTTCCCGAGTTTTTTGAAGAGGATATGATTGTATGCTGTGGCGTATCCAGAGATCAGAATACTTGTAATTCCGATAATCGTGATCATCACGAGAAGGTTCTGATCTTTAATCGCTCCAGAAGAGATTCCCATTGAGATCAAGATAAAGGAAAATTCACTCATTGGGATGAGAGTAAGTCCTGTTAGGAAGTTGTTTTTTTTAACATGACCCATGAGTTTCATCAGCCACATTGTAATTCGTGGTTTGAAAAAGATGACGGCAGCCAATCCTGCTCCGATGAATGGTCGATTAAGGTTGCCATCAAAGGAAATCTGACTTCCCAAGAGCACGAAATATACCACGATGAAAAAGTCTTTGAGCACCTTCATTCTGCTGGCGATTTCGAATCTGTAGGGAGAAGTGGAAAGCGTGATTCCTCCCATCAAGGCTCAGATTTCCATTCCGAATCCTAAAGTTTGGAAGATCGCTGAGGTGATGAAGCACCGTCCTATTGAGAAAAGGAGGAGAAATTCTTGAGATTCGGCGATTTTTCTAGTAAAATAGGGGAGGAGATATTTTGAAAAGATAAAAAGTCAGATTCCGATTCCGAGGATCTTGAGAATTAGGATTCCAGCAACGTGCAAGCTATCTCCGTCGCCCATCGCTGTAAATGCAGCAACGATCGACATGAAAAGCATGACTGAAATGTCCTGAGAAACTGAGGTTCAGATCGAAAGTCTTCCGTATGTGGTATCGATATCATCTTTATCTCCCAAGAGTTTGAGGATTACGATGGTACTACTTAGTGCGGTTGCTGTTCCGATGTAGAGGGAGGTCATAATATCAAATTTAAAAGCTAACCCAATCCCAAATCCTAAGATACTACTTCCGATCACTTGCAATACTCAGACTGCAACGGTTTTTCCTCAAATTTTGTGGATAAGTGAGGGATTGAGTTCGGTTCAGATGATGAAGAGCAGAAATGTGATTCCTACCTGCGAAAGCTGAGTAAAGGTTTCTGATTCTGCGAAAACTCCAGGGAAAAGAAAAGAGATTACGATCCCCGCGAGAATATATCCGATAATCATTGGTTGCTTAAGGATTTTCATTGCTCCTAGCGAGAGGAGGACGATTCCTAGAATTACAGCGAATGAGAGAAAAAACTGATCCATACCTTTATTTTGTTACTATTGCTGAGATAAAAGATGGTATAAGTATAATTGAAAGAGATTTTTTTCGCAAGAGTCTGCTTTTCTTTTGCTCTTTTTCGCTTGACATTTTTTTGTAGGAGGCATTTTGGGAGAAGAAAGGATTGCTATTTTTCTTGGTATCTATATAGTGATGCTGTTTTTTTACTTTTATATATCGTATTGTATGACTAAGAAATTAGAAAAGAAAGCCTTTCCCCTTTGGGCTAAGGTAGTGATTGGGGTCTGTGGGCTAGGAATCGTTTCAGTAGGAGGGCTATTTGCTCTCAATTATATGAAGGAGGGAAGGAGTTCAACTCCTGAGACGTCAATTACTGGGCAACAAGAACAACAACCTTCAGATTTATCTTCTAATGAACCTCTAAACATGAATGCAGAAGCGGCGATTAAAGACTATTTAACACAAGCAAATCAGGATATTAAAGCTGATCAGGTGGTGGCAAGCGGTGCTACTATCACGGTGGACTATATTGGTAGGCTCAATGATAAGGAAGTTTTTGATACGAGCGTAAAAAGTATAGCTGAAGCTGCTGGAACGTATTCTCCTCAGAGAAATTATGATGAAGGACTAAGTTTTACGGTAGGAGCTGGTCAAATGATCAAGGGATTTGATGAAGCTGTCGTAGGTATGAAGGTTGGAGAGACAAAAACTGTGCATATCCCTGCTGAGAAAGCATATGGAAAGAGAGATGAGAACTTGATTCTTCGTGTGCCTTTGGAGCAGGCGGGAGATACTTCAGGAGCTCAAGTAGGAATGAAAGTGCTTGTTTGAGGACAATATCCTGCGACGATTGCTGAAATTACGGATAAGGAAATTGTTTTTGATATGAATCATGAATTGGCAGGTAAGGATCTGATTTTTGATATTACGATTAAGGCTCTTAACTAAGAGATGATTTTATCTTTCTTGAGTATTAGTAGATGGCCACTTTGAAAGTCTATAATGGAGAGCTTTTTGAAAGAGGAAAGCGATGGTATGTCGGTTTTGCTGGCGTTATTTTGACGATTCTTGGGCTTTCTCTTTTTTACAAGACAGGACAAGGAGTGCAGAACATCGTAGGAAGCGTAATTATGCTGATGATTGTCGGAGGCTACCTTTTCTTTCAGTCGAAAACGTGGTCAACGATTCAGCTTACGCTTAGTTCAGAAGGAGTTTTTGCAGGAGAGAGGTTGATCCCTTTTGCTGTGCTTAAATGATTTGTTGTAGAGATGGAGAAAAAGACTGGGAAACTCAGAAATATCGTCTTCGTCTTTGATAAATCGGTTGAAATCTTTACACTGAATGATACTCCTGAGCATCAAAAACTTTTTTTTGCAGAGCTAGCAAAAGTAGTGCCTTTCTTGGAATCTTATGAGCAGGGGTTCGTGGAGAAATTAATTAGAAAGATAAAGCTTTAATTCTGAATATTTTATAGTTGTTTGGTAGGAAGGATCTCTGAAGAGGAGATTCTTTTTTTGTGCGCTTGGAAATTTAGCAAGCTATTCTTCTGCATTAGAAAACTCTTTGCTTACAATGTTTGTAGAAGCTGTTTGTCGAAAGTTTTTTTACCCTAAATGGGAGTTTTTTTGGTTTTTTTGTGTTGGAAATAGTGAAAAACTGCTACTTTTCTCATTTCCTAGGGCTACAATCGTTCCTTGTAGGCGTGTAAAGGCGAATTTGAGGGGGCAGAGGCATGAAGGTTACTCTTGTGAGGAGGAATCCTCAACATATATGTCTTCATCTTGACTAAAGAATAAGCATCATTATATTTGCTTTAGTTTTTTATATGTTTTGTAAGAGCTATGAAGATACTTATGCTTATTTTGCTTATGGTGCAGCTCGCTTTCTCCCAGAAGATTTTGCTTTCTCATCCTCATACTCCAGATGATCAAATAGGGAGAGCGCCCCAGACATACGAATCCACGGCTCAGTCAATTCCGCATGATCAAGGAAATGTCAAAAAAGACGCCTCAGAGAAGCAGCGTCAGGAACAGATTAATGTTCACAAAGAGTCACCTCTACAACCCCTTCCTTCTTGATACCCTAAGAGAGAGGCTAAGGACTTACCTGCCTGTCAACATCTGGATATGACGAAGAATTTCTCCAAAGTTAGGAACCTAGAAGACTTTATGGAGAACTGTAGCTTCGAGGAAGGATATAGCGTGTATGATAATGCTGGTCGTGGCGGGACACAAGACCAGCTCAGTATCCTCAAGGATGCGATAACCGACGCTCGAGGATCAGAATCTGATGAACGCAGGTTTTGTAAGTCCGATCTGACAGATATAAATCTTTTGCCAGATAAAGCCTATCAGGATTTTATTGCCTTTCTAGAGAAACATCGCCTTAGACAGAAGTCTTTGTTGGCGATCGTATTTCCTGCTCCGCTCGATGAGTTTTATTCCTTCTGATGTAAAGATACTAAACTTTGTGATCTTCTGCTAAAGAAACAACGGTGAGAAAATAGATCCCATTACTATGTGCTAGGAACAATTGATTATAGATATACCTGAAATCCTGTCCTTGATATTGATGATAAGTTATATTGGGTATGAACATATACGTCATTAAAGAAGACTTGACTTGCAAAAGAGTCAGAGTGGAGGGTAAGCTCACTCTCTGGAGATACTCTCTTCGTAAGTAGATTTATGGACAATCAGATCGTCAATGATCCTAAGCTCTTAGCACAAGAAGAAAAAGGGTATTGACTGACGAAATTTAAGATAAAACGTTGTGAAATCAACTTATGAAAAATCCCTCCTTCCTAGATACAGGAAAGAGGGATAATTTTTTAAAGGGTTATATTACTTTTGTTTTTGCACATGGATGAAGAAGACATCAGAGGCAGAACTCGTTGAAATCTTGCGATTACCAAAGGTTTTGTTCTGAGGTTTCCCTCCATTATAATCCGTGTAGTAGATGGTAGAGTTTGTAACGCTATGAACGAAACCAACGTGTCCAGGAGGTTCTATATGATGAGTCCCTGGAGAGAACCAGATAATATCTCCTGGTATAGCACGACCTTGTTCGATGTCATAGCCAGCACCATCTAAGGCATCTTTCCAGTCTTCAGCATTTCCTAAGCCACTCGGGAAAGCGTTCCAAGTTCCCCACATTTGGTTAACCTTCCACGCTACCCAAGCTGTGCAATACCTATCGGAGTATCCACTGTTATTGCTACTATTATAGTCGTCAATAGCAGGTCCAGCATTAGAGTTTACCGAGATGGATTGAGCAATTCCCAAAGGATATTTAGGATCATCTCCATCAATTGAGAATGCGATCTCACAGCGATAAGTCCCAACTTCTCGGAAACGATCTTTCATAAACTGGAATGTTACTCCAGAGCTGGATACGTCTATTTTATGCATCTCTTCATACCCTGTAAAACCTAGAGGAGAGTTGAATTTTACAAAGACTCTCACCCTCGGATTTGCAAAATGCGGAAACGAGGAATAAAATGTAAACGTAAACCCAGTATCATACACATAACCTGAATACGGATAAACCTCGAACGTCCCCAGAGAGCTGGAACTTGCGTTCCTCAGGGTAGAAACGAGATTGCCTTCCCCAAGAGGACGATTGTCCTCATTAGGGGTATTCTGTTCACACGAGGTAAATATCCCTGCGGATACTACCATCATAAGCATCAATATGAAATCTTTTGTTTTCATAATTAATTTTGCTTTGTGGAATATTGAAGTGGGAATATTCCTGTTTTGTGACTCGTGAGGTAGTCCACTCCATCTACCTTTGAGGAATCATGAGTAACAAAGTCTCCCTCTGACCTCGGATTTCGACTCTTTAGCAAGAGTTTTACTTTCAATTTTTTTAATTTACAACATAGTATTTATATTTTTTTGTTATCGTGAGTATATTCACGAGTTTTAAAAGGTGATTGCAAGTATAATTTTTAGTAAAAGTAGTCTGCAAGAGGTATTTCCCCGTTGTAAAATCCTTCAAAACGTCTGCAAATACAAATTTCCCGTGGGGGGATTTTGCTAAACTAAGCGATTTTCTTGTAGTTCGAGGGGAAAATCGGGGAGTGATTCAGATTTTTTTAGAGTTTTTCAAAAGCTTTGTCTATTTTCTGGTTTACGGAATCCTTGACAGAAGTTTTTGCGTCTTCTTTGAGCTGATTGTATTGTTTATTCAGCTCATCTTTAGCCTGGTTTGCTAAGTCCTCTGCTTGACCCTTAAGCTGTTTTACTCCCTCTTGGGTGGCGGTGATGGCTCCTTGGACTTGCTCGTTTTCTTTGATGAAATCTGCAGTTTTCTCTGAGACATAGTTCATTCCAGAGGCGGCTATTTGCTGAGTTTTTTGGTATTCAGGGGTGTCGGTAATCGTTTTTCGTGAATTACATCCTGTTAATACTAAGCTTCCTATGATGAGGATCAGCATTCTTTTTTTCATAATGATTAGATTAAGAAAAAATAAAAAAGTCTGACTTGGTTATGAGGTTTTGGACCTAAAAATCAAGGGGGAATTTTGGGAGTGAGCGTCTTTGATGCAGAGGAGGCGGTTCCTTTCTTTGAGTACCAGATTTTTCTTTTTGAAAAACTCGCCTAGTAATCTCACTAAGCGAGTTTGAATGATTCTAAACATGTATTGTGGTTACAAGAGCTGTCCCGCCAAGGCGGCGAGAGGGCTCCGCTCTCCATTATTGAGATGGATATGCGCAAATTCAGGTTGCCCTCGGAATCGATCGATCAAATAGGAAAGCCCATTCGATCGTTCGTTTTGATATGGCGAATCTACCTGACTAATATCTCCAACTAACACGATTTTTGTCCCCTTTGCAGCTCGGGTTATGATCGTTTTAATCTCGTGAGGCGTCAGATTCTGTGCTTCATCGATGATGATGAAACGGTTTGCTAGAGATCGACCACGGACGAAGTTTAAGACGAGGGTGCTGATCTTCTGCTCCTTTGCCCAATCTTCGACGGACTGGCTCGTTCCCTTCTTTTTCCCTTTTTTCTTTTCGTTTGCCTCTTTTATTATTGCGAGGTTATCGAAGATAGGTTTCATGTAGGGATCGATTTTCCCGTCTAGCTTGCCTGGCAAGTATCCTAGTGTCTTATTGCTCATCTCTATAGCGGGACGAGCAACTAGGATCTCCTCGTATGGATCTTTTTGATGAAGTGCTGCTGCAAGACTGATGATGGTTTTTCCTGTTCCAGCGTTGCCGGTTATTGCCACGAGCGGGATGTTATCATCAAGCAGAGCATCAAAGCAGAATCGCTGTTCATTATTTTTAGCTTCGATGCCGTATACGGATTCTTTTCCGTTGTAGTATCCTACTGGCATTAGGTATTTTCCACATTTCCTGACCAAGTATTCTTTTCTATTTTCTCCTTTCCCTGATAGGATGATGAATAGAGAATTTTCTGGATACTTGCTCAGACTTTTGGGGACGCGATATCCATTCGTATTCCCCATATCTTCAGTTAGCTCCCCCCAGCCTTCGCTTCTGGGGCGGTAGGAGATCACTGATGCTTGGATACTTTCTAAATCTTTCACGCTATCGTGGCGATAAGATTCAGATTTCACTCCTAATGCTCGTGCTTTGATCTGGAGATTGATGTCGTTTGTTACCAAAACGACGTTTTCTCCTTTCCTTTTTTTGTCCAGTGCAATTGCCAGTATCCGATGGTCTGGAGTATCTTTGTAGAATGATTTTTCCATCTCTCGAGGATAGTCGACCCCCAATGCAACTGTTAGTTTCCCTCGATTTTCTCCCAAGGAAATCCCTCCGTTAAAGAGCCCATCTCCAAATCCGTTGAATATTCTATGAAATATTCTAGTATTGACATTGTTGGGTTCATTTCCTTTTTTGAATTTATCGAGCTCTTCGAGCACCATTATAGGGATACAAATATTGTGTTTCCCAAAATGTTTCAGGCAATACGGATCATTCAGGACGACGTTCGTATCCAGAATGTAGGTTTTCTTTTCCTTCTCGCTCTCGGTTTCGCTTGGAAAAGATGAAATCTCGCCTTTTGTTTTTCCTTTTTTTTTGTTCTTTTTGTTACGAGGGGAATCCTCTTTTCTTTTTGCCATTTTATTTATTTTTTGGATGTATTTATTAATTAGTTTTTTTATAGTTATTTTTAGATCAAAAACAATGTTTTTTGTTTTGTCAATTTTTTTGTCTCTTGAAAGCAATTATGTCTAGATTCTCTGCTGGCTGTTTTATCTAGGGGAAAGTCTTTTTAGGAGCGAAAGAAGGAATGAAATCAGATTTTTTTCTGTTGAATAATGTTTGGATTCAAGGCAAAAGAAGAGTTTTTTTTCTTGCTGTTGAAAAACCTTTCAAAATCCCTACAGTTGTCTCCTGTTGAAAGATTTATCTTTTATTTCGATTCTCCAATATGCAACTTACGATCGCGTCCTATCTTCAAGGACTGAAGAATAAGGATTTTAGCCCCAGAGAAGTCCTCCTTGATTATCAAAAAAGAGCTAAAGCTCTTAATACTGAACTTAATGCCTGTGTGCGTTTTAATGATGAGTATGTAAATGCTCATTTTGAGGAATTTTCTACTAGAGCATTAGCTTCGCTTCCGATTATGGTTAAGGATAATATCCTCGTTAAAGGGCAGTCGGTCACTTGTTGTTCTAAAATGCTTGAAGGTTATACTGCTCCTTATTCAGCGAGCTGTATGCAGAATCTAGAAAAGGCTGGTTGCTTGATGATTGGTCAGACAAATATGGATGAATTTGCCATGGGAGGCTCTACGGAAACTTCATTTTATGGGAAAACGCTTAATCCTGTTTGTGCAAGTCGTATTCCTGGTGGAAGTAGTGGCGGCTCCGCAGCTGCGGTTGCTGCGAATATGGCGATTGCTGCTCTGGGGACCGATACGTGAGGAAGTGTTCGTCAACCAGCAGCGATGTGTGGGATTGTCGGATTTAAGCCAAGCTATGGTGCGATTTCTCGCTATGGGGTGGTGGCAATGGCGTCGTCCTTGGATCAGGTTTGAATTTTTGCAAAAACGGTGGAAGATGTGCAGCTTTTGTTTCCTTATTTAGCTGGTTATGATGATAAAGATGGGACTTCATCTTTGCAGGCTGAGTCTTTAAAAGAAAAAAAATCTGAATTCCCTTCTCCGTATAGGATTTTTGTGCCAGAAGAAGCTCTCTCAGAAGGCGTTCATCCACAGATCAAAGAGCTTTTTTTGCAGAAGTTAGAAATCCTAAAGGCTCAGGGACATCAAGTGGTTATTGATTCTTTGCCGATTATGAAGCATGCACTTGCGGTTTATTATGCCTTGATGCCGGCTGAGGTAAGCACGAATCTTTCTCGTTTTGATGGGATTAGATTTTGACATCAGAAAGAAAGTCAGGATTTTCCTTCTCTTGATGCGTATTATTCGACGATTAGAGCGGAAGGGTTTGGGGAGGAAGTAAAGAGGAGAATTCTGTTAGGAACTTTTGTTCTGAGTTCTGCTAATTATGAAGGCTATTATCTCAAGGCTTTACAGGTGCAGAAGCTTTTGAAGGCTGAACTAGCGAAGATCTATCAAGAGTATGATGTCTTATTGACTCCAACGACTCCAGAGCCAGCTTGGAAATTGGGATCAAAAACTGCCGATCCTCTCAAGATGTATCTTTCTGATCTCTATACGGTTCCTGCGAATTTGGCTGGGTTGCCTGCGATTTCTGTTCCACTGGGAATGGTAGATGAAGAGGGAGATCAGCTTCCTGTTGGAATCCAAGCGATGTGAAAGCAATGGTCAGATTTAGAACTGCTTGATTTTGCAAGCAAGTTGTAGGTTTTTTTGTTGGTGCTTATTTGAAGGATGAGTACTTGGTTTTTTTGATGGAAGTTGCGCTTGAGTGGATTTTCTCTTGATTTCTTTTCTTGAAAGTGTATACTTCTGTCATCTTTGCCGTAGACCGTAGGTCGTCCCTTTGGGATATAAATTGTTCCTACCGAGGTTATTCTTACTACTTTGAGAGATCAGATTTTATCTTTTCTTTCAAGACATGAGATAACTCTGGCAATGAGTTATTTTTTATTTCCAGGTTTTACACTACTCATGACAATCTCAAAAGAGAAAAAAGTAGAGCTAGTAAAACAGTATGTTCAAGACTTACAATCTGCAAAGAATGTAGTGATCCTTCAGCAGTCTGGAATCCCAGTAGTGGGAGCTACTCAGATGAGAAAAGGAGTTCTTGAAGCTGATGGTAAATTTAATGTCATCAGAAAGAAACTGTTTTTACTAGCGCTCAAGGAAGCTGGTTACGAAGATGTAGCTGAAAGCGATCTTGAGGGATCAGTAGTTGTTCTCTATGCAAATGGAGATGAATACGGTCCAATGAAGGTTGTTAATAAGTATGCAAAAGAATTTGCAGCTGATAAGGAGCTTAAAGCTTCTATCAAGTTCCTTGGAGGGTGGTATGACAAGAAGTGGCATAATTCAGAATATGTTACTGAACTTGCAAATATCCCATCAAGAGAAGAGCTGCTTTCAAAATTGGCATACCTATTCAATTACCCTTTACAGTCAGTTGCTTGTGTGATTGACCAAATTGCAAAAAAAGCTGGATAGGCTTTCTCTTATCCGAAATTCCTTTTATTTATTATTCTTATCGTAGAAAATGGAATTAACTAAAAATCAACAACAGATTTTGGATCTTGTAAAAGAGATGAATGCTGTTGAACTTAATGGGCTTGTAAAAGCTTTAGAAGAAGAATTTGGAGTAACTGCTGCTGCTATGGTAGTTGCTGGAGGTGCTGCTGCTGGAGCTGGTGCTGCAGACGCTGGTGCATCTGACGCTATGAATGTTGAGCTTACTGATGCTGGACAACAGAAAATCGCTGTAATTAAGGTGGTTAAAGAAGTTCTTGGACTTGACCTTAAGGCTGCTAAAGAACTAGTAGAAAAAGCTCCTGTAATCATCAAGGAAAATGCTAAGTCTGATGAAGCTGAGGCTTTGAAGGCTAAGCTTAGCGAAGCTGGAGCTACTGTAAACTTCAAATAGTATTTGAGGCTTACTAAAAGTCAAAAATCAGATTTTTGATTTCTTTTGTCTTTTGAAGAAGATGAAAGAGTCGGCGAAGCCGATATTTTATTTTGATTTTAGCACTACGGATGTCTGTATCTGTTCAAGATATTGTTGCTGCACAGGCTCACATTGGGACGCTTAAAAATGAAGCTCATCCAAAGACAAGTAAATACTGGTCTGAGGTAACCAACGGAGTGGTAGTGATCAATCCAGAAGCGATTGCTGAACAACTTGAGGCTGCAAAAGCACAGGTTGCTGCTGCGAAGGCTGCTGGAAAGGAAATCTTGGTTGTATGTGAAAAGAAAATGTATGCTGAAGAACTTGAAGCATTAGCTGCAAAGGCTGGCGTTGCATATCTCAACTACAAGGTTTCTGGAGGGTTCTTAACGAACTTTGATACCTTCAAGAAAAGAATTGATTCTATCAATGAAATGGTGAGCTTTATGGAAACTGATGCTTTCGCATCTTTGACCAAGAAGGAGCAGCTCGTGTACAAAAGAAACTTTGAAAGAGCAAATAAGGTCTATAAGGGAGTAACAAAACTCAAGAAAAGACCAGAACTCGTAATTATTGTGGATGGAAATATGCTTTCTACCCTTATCGATGAAGTAGAGAATCTCAAGGGAAAACTTGAAGCGATCGTAATCGCAGGGACCAACTTCTCAAGATATTGGCCAGAAAACGAGATTATTACCACCAATATCAATAGTTATCAGAGTTTGGATTTTGTTTTGAAGTCTATTCTCCTCTAAGAGAGAAACTTATTGGGACTTTTGTCATCGCGAGGCGAAGCCGTGGCAATCCACTTAAAAGCAAATTTGCTTATTCTCTGGATTACTTCACTTCCTTCGCAGTGACAGAGTTGATCTTTATTCCTCTTATTTCCTATGCAAGAACAACTTTACTTGCCGAGTTCGTCTGAAAGAAAGAGAGTAATTACTGCCTATCTCTTGATAGGGCTCTTCTTTTTTTCAGGCAAGGAGGAATTAACGGTGTATGAATACTTTCATTATAAGCAGGCGATTGGATGGTATATGTCTACGATGGCATTGCTACTTTTCCAACTCATGCTCGTATGGATCCCGATATTGAAATGGTTGCCTTTCCTCTTTGTATTGGCAAATCTTGTCATTGTTGTGATCTTTGTCTTTGGGGCATGGAAGGGGCAGTATGCTCGCGATGTTCTCCAAGAGCATAGATGGAAGATGTTTTATGCTGATCTCGGTGGACGATTGTTGGGAATCTTTGATATTAAGAAGAGGGTGTATGGAGATATTGCAACTGATACTACTGCAGAGAATCTTCCGACGCAGGCTCCCTCACAACCTCTTAGCTAGCTTTGTGGTTTATCCAAACGAACGGAGAAAAAAGTCAGATTTTTTATTCTGGTTTTCATTTCGTTTCTTTTGATAAATGGCAACACTGCTTGTCTTTTATTTCTTATCTTATATGTAGGATGAATATCGATATTAATTTGGTAAAGCAGCTCAGAGAAGCTACGTTTGCTCCACTTGGAGACTGTAAAAATGCCCTTGTAGAGGCTGGTGGAGACCTTGATAAAGCTCAGGAAATCTTGAAGCAGAAAGGAATCGCAAAAGCAGGCAAGAAAGCTGATAGAGAAACCAACGAAGGACTCATCAAGACGCATCAAGCAAACGGAAGAACTATCGTGCTTAAGCTTCTTTGTGAGACTGACTTTGTGGCGAAAAATGAGACGTTTCTTCAGCTTTTTGATAAGATCTTTGCTGAACTCGAAGGTGTTGCTGGAGAGGTGAGATCGCTCGAGGATCTCGACGCTGCAAAAGTAGAGACGATCAATACGATGCTTGCTGAAGCGGTAGGTTCTATCGGAGAGAATTTGAAGATCGGAGAGCTTTTCATTACTGCTGGAAATGCTTATGCGTATGCGCATCCAGGAAATAAGGTCGTTTCTTTGGTGATGTATAGCGGAGATGAGGCAACTGCGAAAGAATTAGCTTTGCAGGTGGCTGCGTTGAATCCTGACTATCTTAATTTTGATGAGGTGCCTGCCGATGAAAAGGCTAAACTTGAGGCGCAGTTTACTGAAGAGCTTAAAGCAGCAGGGAAACCAGAAGCCATGATCGCAAATATCGTGAAAGGAAAGATTGATAAAGCGTTTGCTGATGTGGTACTTCTTGAACAGGAATATATCAGAGATGGTGCTAAAAAGGTGAAGGAAATCTTGCCTGCTGGATTTGAAGTAACGAAATTCTATAGATTTACGGTGTAGTTTTCTGTTAATGATGCAAAGAGAGGAAGTCTGATTACAGAAAGGGATCAGACTTTTTTCTGTTTAAAAGAGGTTGAAATCCTGTATTTTGTCGGCTCTTTGGTTGGATCTCACTTTGTCTTGATATTTAAACTTCCTAGCGAGTATTCTAGGGAGTCTAAAACAACAAAAATTATATGAAAACATTCAGAAATTCAATCTTCGGATTGTTTGTGATGATGTTCGTGGTTGTAGTTCTCTTGAGCTCCTGCGAGAAAGATGTGGATCAACCAGTGGTTGTTCCTAGTGCTGAAACCCTTCAGGATGAAGTTCTTGACTCCACTCAGGAGTTAAGATCATCTAGTGGTAGTATGTATTGCGGGATTGTGATGCGAGAAGGGAAAGGGTTCTCGGAAGATACCCATGGTCTTCCCCAGGAAACTCATTATCGTTTCTACGTGGCTAATCCCTCCTCGGATATTACGCGGGTTGATGTCGTCCTTTCTATCCCTTCGGGGGGAACAGAGGTACGTCCTATGGTCAAGAACGGTAAAGGCAACTTCTTCCATGAACAGACGCTTCGTCAGCATGGGAGGTATGATGTGAAGTATCGAATCTTTAGGCGGAGGTCTTTTTCCTATCAGGTCGTTGATCCCAAACCTAATTATGTCGATAATACTAGGGTTTGGTGTGCATCAAATGCTCTAGGGATGTATTGGGTGTTTGCAGATGGATCTTCCCGATCTAATAATTCAGTCTATAAACAGGTTACTGTCGAGAAGAAACCAGGTGATAAACCAGGCAATGAAAAAGAATGGAAACGATTTAAGTGGCTGAATGGTGAGGCTGATGGACGAGGCTTCGGAGGAAGCGGATTTGGAGAGGGGCTACATACCAAAGGAACTTCTGAGGAATATGCTTTGGATTACAACTTGTATCCTTATGATTGGAAGCCTGGTTCTTCGATGGAGATCTATACTGATATCGATAAGGGCACGGCACTTTACTCTCCCCTTGATGGGAAGGTTATCAAGCTAGAGAATAATCCTAAAGCGAAATTTGGGAAATATGTTGTGGTTGAGCAGAAACTTGGTGAAAAAACCTTTCAAGTCTATTTTGGGCACCTAGAAACTATCTCTGTGCAGCGTGGACAGTATGTTATTGGTGGACAAACTGTCGTTGGGACTCTTGGAAATACTGGAGCTTCGGGTGCCTACCACCTGCATATTAGTGCATGGGATGTGACGCGGTCAAAAACTTCAATACAACATGGTATGTTCTTGAAACCTGGAGAGTGATCAACGTTAGATTTGTGAAAAAGCAGTGGAATTCCCACTGCTTTTCTTTAGAATGCGGATGTTTTATCTTCTTTTCTGATAAGAATGTGAATGTTAATGCTTGGGATGTTTGCGATGTATAGCTTTCTTTTTCCTGCGCTCCATCAAGAATCTGAGATTCCCCACCAAAGGGAGACTGTTGATGGGCAAAAACTTACCCGGGAGGGCTACCCTGCCATTCCTGATAACACCGAAGTCCTTTGGTGGGACTGGAAGATTAACTATCCTCCAATGAAGAAAACTTTTTTAGTGAAAGGGGAACTCACGAGGGACTCGTTCTTATGTACTCGACCCCTTGAATGTGATGAAGAGAGAGGAACTCTTATGGAAACTTGGGAGATTGTCTCTAAAGATAACAAATCTACTCCTGAAAAGAAAATTTATTTGGGGGTGACTTCCTGAGCAGTGATTTATTCTTCTCTTCCTGTAGAGAGGAAGTATCCTATTGCTAACTTTTGAGGTCATAATCTTCGTAAGATTGCTAATAACGGTAAAGAATATTTGTTTTTTGTCTCTTATAACAAACCAGAGAATTGGGAATTTAGGAGCGATAACTCTATGGTAGAAGATCGGCGAATCGTGGCTGGGCAGTAGGTGGTTAGAATGCGGATGTTTTATCTTCTTTTCTGATAAGAATGTGAATGTTACTCTTGGGGATGTTTGCGATGTATAGCTTTCTTTCTCCCACGCTCCCTACTGAACCCGAGACTCCTCATATATGGGAGAGTGTTGACGGGCAGAAATTTACCCGGGAGGGCTACCCTGCTATCCCTGATAACACTGAAGTGCTTTGGTGGGACTGGAAGATTAAGTATCCTCCTGGGACAAAAAGGGCTTTTTTTGTGAAGGGAAAGCTGAAGAGAGAGGTATTCTCGTGTGAAGAGCTGAATGAATGTGAGTTTCCTCGATCAAAGCCTCGCACTGAACAGTGGTTGATTGTTCCCAAGGATGAGAAGTCTATCCGTGAAGATGAAGCAATTTATCTCGGCATGACTTCTTGATGAACTATTTATTCTTCTCTTCCTGTGGAGAAAGGGTATCCCATTGCTAACTTTTGAGGTTATGATCTTCGTAAGATTGCTAATAATGAGAAGGAATATCTCTTTTTTGTCTCTTACAATAGATTGGAGAATTGGGAATTTTGGAGCGATAGCTCTGTCGTAGAGGATCGACGGATCATAGCTGGGCAGTAAGTCAGGAAGAAGCAGCTGAGGGCTATTTCAGAGAGTGTTTCGTTCTCAACGATCATTTTTATGGGCTGCATTTTGCGAGAGAAGCAGTGATTGCGTTGTCTCAGCAGTATGATTTTGAGTGGTTCCGTTCAACTTTGGACGGGGTAGGGTAAGGGTTTGCTCAGTAGGGTGGGAGAAAGGATTGGAAATGGGGGAGTTTCGCTTTTGAGAGCTTTGGTTTCTCGTCGAAATCACCTGATTTCCTATACGCCATATGAAAAAACTTTCAAATTCTCTTGATTTCAAATCCTAGAAAACTATACTGCAAAGCGATCGGCGAATCCAAAAAGTATCTATTTTTTTATTTATTCTTGGTGAAACTTATGCAACATTATGAATTGGTGCTCTTGCTCAATGCAAACACTTCTGAAGCGGACAGGAAGGCGTTTTTAGAAGGTCTTGAATCGAAGTTTGAAGTAAAAGAAAAGGATGAAATCTGAATTCAGAATCTTTCTTTTAAGCTTAAAGATGGGAATACAAAGGCGTATTTTGTATCTTACCTTCTCAACCTTTCTCCAGAACAGGTGAAGGAAGTAAAGGCTGCTCTTTTGTATAACCAGGCACTTGTAAAGTATGAGATTTATAAGATGGGGAAAGATCAGAAATTTTTCCATTTTGAGAAGCTTCAGTCTGAGTTTGATAAAGCGATTGAAGAAATTAAGGAAAGAAAGTATGGACAGAAGATTAGTTTCTTTGCAAACGAGAGAAATGCAAAGTATATCAACTGGAAATCACTCCCTGTTTTGAAGTATTATCTTACGAGGTTTGGTGATATTAAGCCAAGAGCTTATACTGGAAACTCTGTAAAGATTCAGAAGAAAGTAAGGCAGGAGATTATCAGGGCAAGAACACTTGGATTGCTTTCCTTTATTAGTAGATAGTTATTGTTGTTTTATATCTTAGTATTGTATTCTTATGGCATCTAAATTAAGAAGAATTAGAAAATACCAGTGGACAAAGAGAATTAGGACTCATGGATTTAGAGAGAGAATGAATTCTTGGACTGGAAGGAGAGTTTTGGCTCGTAGAAGAGCTAAAGGAAGGCATCAATTGACTGTTCAAAGACAGAAATAGATTGGTTTCTTTATGATAAAAGAGAAAGCTGACTGTGAAAATGGTCAGCTTTTTTTCGTAGGGTTTGATGTTGACTCTTGGTATAGAATTTTTATACTGAGGGCAATTCTTTTGATCTGTTTGATCTCTCCTTTGCTGGAGGAGGGGAAGGTTGGAGGAGTCTGTTTTTTATTCTTTTTCTTGATGCGATGGGTATGAAAGCGTTTAGCTCGGAAGAGAAAGTGCGTTTGCTTGTGGATTGTGATCCTGGTGCGGACGATGTATTTGCGTTGTTATGGTTGTTGATTCAGCATAAGTTTGCTGAGATTCCTTTGGAGATTGTCGGGATTACGACCGTTGGAGGGAATGTAGATGCAGACAAGACCTATGAGAATGTATTGCGTATTTGTGCAATGGTTGATGAGCATCGTATTCCTATAGGAAAAGATCATCGTCAAATTGTCTCTGAAGATGCTTCCTACATTCACGGGAATGATTGAATTGGAAACTTATGAGCGATGCTTCCTCCGGTGCAGTATCCTCAAGAAGTCCTTGACAGTGTAACGATGATTATTGAGGCGTTAGAGAAGTATCCTGATGAATTGACGATTATTGCTACGGGACCGTTGACGAATCTGGCACTTGCAGAAGAGAAAAATCCATGAATCCTGAAGCGTGCCAAGAAAATTATTGCTATGGGAGGGGCGATTGAGATGCAGTGAAATGTTACTCCTACAGCTGAATTTAATATTTTTTATGATGCTCCGAGTGCAGCGAAGGTATTTTCAGCGAGTTCTCGTATTGTGTTGCTTCCGCTAGATCTCACTACAAGTATGCCTTTTACGATGAGGGATATGGAGAATTGTTTCATCTCCACCGTTAACAATACGCAGAAGCAGGAATTTATGAGGGCTTTGACGAAGTTTATTATTGCTACGAATATGAAGTTTAGAGAGACAGGTTATCAAGAAGGCTTTTATATCCACGATGCCCATACGGTAGGAATTTTGCTCTATCCTCATTTGTATAAAGGTTCATTTATGCAGGTTAATATTGAGACGCAATGAGAATATACTAAGGGACAGACGGTTGTTGATAGGAGAAATATTGTAACACTGCAGACAAACTGCTTTGTGGCGATGGAGTTTAAAAAGGAGCAATTCTTGGAGGCAATAACACAGGACTTCAAGTTGTTTGACTTCTCTTAAAACGAGTGCTTTTTCTCTGAGATTTGAAGAATGGAATTGGGACTTTTTGGTGCTGAAGGGGGACTTTTGTTTTACAAGGGAAAAGAAATCCTGATTCTGTTTTTTCTTTTTTTTGAAAATGGTCTTGATTTTTATCGGTATAACGCTATATACTTCTGTTGCATATTGGTAAATGGAATGGGATGGTGGTTTATTGGGCGTTTTGATTTGCCTTTTTGTTTTTCTTTGCGTGATAGGATCCCGATCACCTTTTGTGCAAAATAGAATATGATTGTTGAGAGTGAGGCTTGAACGATGGATGTGGTCTTTGTAATAGAAAGTTCTTCCTCCAATTATTATCTTCTGATGCGAATACAATTATCGAGCTATTTTGTTTATTTTTCATCTGACTAATCGCCTATGGTAAATAAAAAACGACCTTGAGCGGAGAAGGCTGCTAAATCAAGCAAGTCTTTTTCTGTTGTAACAAAAAAAACTTCAGCTACTAAAGGGACTGTAGCTAAGAAAGCAAACTTGTCTGAGATGATCGTGGAGACGAAAAATGTTGCTTTGAGGCCAGAAGTGCAGGAGCTCTTCAAAGACTATTATCAGCAGGATGGAAGAATCTTCCTTACCCAGCCAAGAAGCTATGGAGAATTGCCTGATCTTCTTGAACTTCAAAAGAAAGGGTATTCTGATTTTATCAATGTTTATCTTGGAAAACTTTTCTCTGATGTGGAGAATATCTGGGATATCGCTGGAGATAAGCTCTATGTAAATATTTCAGATTTGCAGGTTTCTGAACCAATTGAAACCGTAGATACTTGTAGGAAGAAGGAGCTTACCTACTGAGGAATCATCACGGCTAAGGTAAAGCTTGTGGAAAGAATTGAAGATGAGAAAACAAAGAAAGTATCTGAGAAAGTGCTTTTCAATAAGAGGGCAAATATCGGTATTCTTCCTTTGATGACGCCTTCAGCTTCTTATATTATCAATGGAGTTGAAAGAGTGATTATTTCTCAGATCATTAGATCATATGGTATCTTTTATGGAAAGAAAGATTTTTGGTATTCTTTCAAGCTTATTCCTGAGAACGGACCTTGGCTTGAAGTAAATGTAGAAAAAACCTGAAATATCGTTGCGAGAATCAATAAATCTAGAAAGTTCCCTATCACCTCTTTGCTTAGAGTGTTCGGTCTAGAAACTGATGAAAGTATTAGAGAGGTATTTAACGGTATCTTGGAAGAGGAAGATGTTGATTTTATAGATATTACCTTGAAAAAAGACACAACCGTTGATGCGATTTCTGCGGCAGAGCATATCTATGGAAAACTCAGACCTGGAGAATTGATTGATGCGCAGTCAGCTTTGGATTATGTGAAAGGGCAGTTCTTACTCCCTGAGAGAATTTATGTTGGAAATATCGCAAGGAGAAAGATCAATGCAAAACTCAATCTTAAAAAGCCTTTGAAATCCGCTGAGGCAAATATTTTTGATGGAGCAGATCTTGTAGAAGCAGTGAAATACCTTGTGAATCTCTGTAATCAGAAAAAAGGTTTCTATACTGATGATTCTGATCATCTTTCCAACAAGAGAGTGAGAACGATGGGAGAAATCTTGTATTCTCACTTGCAACCTGTGATGAGAAAGTTTGTAAAGAGCGTGAGAGGAAAACTTTCCGTTCTCAATATGGAAAATCCGCTTAAAATCACTGATCTCGTAAACTTCAAGATGATTGATAACTCTATCAAATCGTTCTTTGCGACCAGTCAGCTTTCTCAATTTTTGGATCAGATCAATCCGCTTTCTGAAATTGAGCATAAGAGGAGAATTACCGCTCTTGGGCCTGGAGGTTTGAAGAGAGAAACTGCGAAATTTGAGGTGAGAGATGTGCATCCTTCGCATTATGGTAGAATTTGTCCGATTGAAACGCCAGAAGGACAGAATATTGGACTGGTAATTTACCAATCTCTCTATAGCAGAATCAATGATGAAGGATTCCTTGAGACACCTGCATTGAAAATTTTTCGTGAAGTAGAACCAAAAGCTGAATCGCTCGTTGGTAGAATCGCTCATCGTGATATTTATGAGCTTGATGCAAAGGGAAATCCTAGCAAGAAAGTGATTATCAAGGAAAATGAAGTGATTGATGAGAAAATCGCTGCCACTCTTGAAAAAGCCTATGGGAAACTCAAAAAACCGATCGCTGTAAAGCCGTTCTTTACTGGAGAAATTGAATATATCTCTCCAGAAATGGATGAAAAATGCGTAATTGCCGATGCGACCACTCCTTTGGATGAACATAATAATATCCTTTCTACGAGAGTTGCAGCAAGACACTTTAGTGAAATGGAGACCTTCCATGTGAACGATATTACGCATATGGATGTGAATTTGAGTCAGATTTTTTCTCCTAATACTTCTTTGATTCCTTTCGTAGATCATAACGATGCGGTGCGTGCTTCTGTAGCGACTAACCAGCAAAGACAGGCACTTCCGCTTCTCAAAAATGATGCTCCACTTGTTGGAACCGGACTTGAGAGAGATATCGTGAGAATGACGCATGCGGTGATTAAAGCTGAAGCTGATGGAGAAGTGATTTATGTGGATGGAGGAAGCGTTAAAGTAAAATATAAAGACGGAACGAAAGAATATCACTTGACGACTTTCGTGAGATCAAACCATAAAACTTGTATTACTCAGGTGCCTTGTGTAGATTTAGGACAGAAAGTGAAGAAAGGCGATTTGCTTGCAGAAGGACCTTGTTCTGTAAATGGAGAAATGGCAGTTGGAAAGTCGCTTAGAGTAGCTTTTATGCCATGGAAAGGGTATAACTACGAGGATGCGATCGTAATTTCTCAAAGACTGATCAAAGATGACGAATTGACTTCCATCATGATTAACGAATACGAGATTGAGGTTGCAGAGACCAAGCTCGGCCCTGAAGAAACAACGAATGATATTCCTGGAGTTGCGATGGCGAAACTAGGAAATCTTGATGAGGAAGGAATTATCAGGATTGGTTCTATTGTGAAAGGAGGGGATTTGCTTATTGGTAAGATCACTCCAAAGGGAGAAGGGGAGCTTACTCCAGAGGAAAAGCTGATTCAGGCGATCTTTGGAGATAAATCCAAGAATGTAAAAGATACTTCACTCTATATGCCTTCCGGAAGTGAAGGTAAGGTCGTAGATGTGGTGATCCTTGATGCGAAAAAAGGAGATAACCTCATGGCTGGAGTGAGGAAAAAGATCAAAGTTTATGTCGCTTCTACCAGAAAGATTGAAGTCTGAGATAAGCTTGCGGGTAAGCACGGAAACAAAGGTATCGTTGCGATCGTGGTTCCAGAAGAGAATATGCCATATTCAGCAGATGGAAGACCAGTTGATGTGGTACTTAATTCACTATGAGTAATTTCTCGTATGAACCTTGGTCAGCTTTTTGAATCTCAGTTGGGTTTGCTTGCGAAAGAACTTGGTGTGAAGTTTGCAGTGCCTACTTTTGGTGGATTTGGAGTAGAAGATATTGTGAAACTTGCTCAGAAAGCAGGACTAGAAGATAAGCTCAGAACGACGCTTTATGATGGAGAAACGGGAGAACCGTATGCTCAGCAGGTAACGGTTGGATATATGCATTTGCTCAAGCTCGTGCATATGGTAGAGGACAAAATCCATGCGAGATCTGTTGGTCCTTACTCTTTGATTACTCAGCAGCCACTTGGAGGAAAATCCAGACAAGGAGGACAGAGATTTGGAGAGATGGAAGTGTGGGCTTTGGAAGCATATTCTGCAGTGTATACCTTGCAAGAAATGCTTACGGTGAAATCTGATGATGTGGTCGGTAGAAATAAGCTTTATGAATCTATTATCAAATCTCAGAAACCAAGAATCTGAGGATTACCAGAATCATTTAACCTTTTGACCTATCTCTTCAAGGGATTGGGTCAGAATATTGTGGCTTTGACTGCTGATGAACTGGAAACGATTCATCAAGAGAGAACGGAAAAGATCAAAAGCCTTGGACTCTCAGGAATTATGACAGCTTCTCTTGAACATGAGACTGCAACTGATGAAGAAACTGATGAGTCTGAAGACAAAGGAGAAATGATTGGAAAAGTCGTTGAAGACCTTGAAGATTTTGGGCAGATTGAGTAATGAGTGCTTGTTATTGCGAGGCGAATGAAATGAGTCGTGGCAATCTATTTGGAAGCAAATGTGGATTGCTTCGCTTTGCTCGCAATGACGGCAGATATCACCTTTTAACTTTTTATAAGCGGTCCTAATGAACAAAACAAAATTTGATGGATTGATGGTAACTCTCGCTTCTATGGAGGAAGTCGCAAAATGGTCACATGGAGTGGTAGATAATCCAGATACCGTAAACTATAGGACAGGAAAGCCAAAACAAAGCGGACTTTTTTGTGAGTCTATTTTTGGTCCTGTGAAGAACTTTGAATGTAGCTGTGGAAAATATAAAGGAGTCAGATATAAGGGAATCGTCTGTGAAAGATGTGGAGTAGAAGTGACAAGTTCTAGAGAGAGAAGAGTGAGGATGGGACATGTAGATCTCGCTTCTCCAGTTGTGCATGTATGGTATAAAAACTCGCCATCCGGAGGTATTCACCAGCTCCTTCAGCTTTCAGCTAATGAAATAGATAAAATCCTGACTTTCGTGAAGTATGTTGTCGTAAAAGAGGTAGATGATGCTGTCAAAGATCAGATAAAAGAAAAGATCAGCACTGACTTTGAGGCAACCATGCAAGAACTTGATGAACTCTTCAAAAAAGAGACGGCTTCAGAAAAAGATACTAAGAAGCTTGCAGAGGCAAAAAAGCTTTATGAAGAGAATAAAGCTTCATTGGAAGCGGAATTTGGAAGAGTAAAATCGCTAGTAGCTGACCTTAAGTTTGGGGCGACGGTTTTGGAGTCTGACTACAGAAATGTTTTCAGTCAGTTTTCTAAACTGGTTTCTTTCGCTTCTGGACCTGAGGGAATCCTCAAAATGCTTCAAGCTATTGATGTAGAGAAGGAAATCAAAAAGAGAGTAAAGGAGTTTCCATCTATTAGATCGGCTGATCAGAAGAAAAAGGCAATGAGTCTAATTAAACTTTTGATCAATCTCTATGTATCAGGAGTGAAGCCAGAAAATATGATTATCAGAAAGCTTCCTGTGATCCCACCTGACATTAGACCAGTGGTGCAGCTTGATGGTGGAAGATTTGCTTCATCAGATGTGAATCTTTTCTATAGAAGGGTGCTGATGAGAAATATCAGACTCAAGAAGATGATCCAGGTTGGGATGCCTGATATTGTAAAAAAGAATGAAATCAGACTGCTTCAGGAGTCTATTAATAATCTTTTGGTGGGAGAAAAGAATGCTGCTGGTAAAGCAGGAGCAGGAATCAAAGTCTTCAAATCTATTTCCGATATGCTTTCTGGAAAGGAAGGAGTCTTTAGAAAGAACTTGCTCGGAAAGAGAGTGGATTATTCTGGTAGATCAATTATTACCGTAGGTCCAGACTTGAAACTCAGTGAATGTGGACTTCCGATCTATATTGCGGTGAAGATGTTTACGCCGTTTATTATCGGAAAGCTGATTGAAAAGAAGATCGTTTATACGCCAAAGCAAGCGGAAAAACTCATCAAAGAAGGAAATCCGACCGCTTTGAAATTCCTAGAAGAAGTGATTAAAGACAAATATGTGCTTCTTAACCGTGCACCAACGCTTCACAGACTTTCTATTGAAGCCTTCAAAATCAAGTTGATGCCTGGTAAAACAATCAGACTTCATCCTCTTGTTTGTCCTGCGTTTAATGCCGATTTTGATGGTGACCAAATGGCGGTGCATTTGCCTATTTCAGATGAAGCTCAAAAAGAAGCTCGCGAACTTATCGCAGCGGACAAGAATATTATCAAACCGGGTTCTGGAGAGCCAACGATTACGCACTCTCAAGATATGGTGCTTGGAATCTACTTCTTGACTGATTTTTTCAATCCAAAATATCCTGACTACAATACTGAAGAGGAATGGAAAGAAAAAATTCATCCTGTAGCTAGATTTGCTTCTTTTGATGAAGTGATGGCAGCGTTTAACAATAAACAAGTGCAACTCAAAGATAAAATCGTGGTGCTTGATGGTAATGAAGTGCTTGAAACTACGGTAGGAAGAGTGATCTTCAATTCTATTTTGCCAGAGAACTATCCGTATGTAAATAGAAAGATGGGAAATAAGGATCTCAAGAGGCTTTTGAGTGAGGTCTTTGACAAATACGATATGGAGACTACGGTAAAAGTTGCCGATGCGATCAAGGATTATGGATTTAAATATTCAACTATTGCTGCGACTTCAATCAATGTCCTTGACATGAAGGTTCCAAAGGAAAAAGAAGACCTTTTGAGGGCTGGTGATTTAGAAACTAATACGGTATTGAAATATTTCTATAAGGGATTCTTTTCTGAAGCAGAGAAACATAGATTGATCGTAAAAATCTGGACTGCAGTGAAAAAGGATGTAGAAGTGAACTTGAAATCTATTATTGGTGCAGGAAATAACCTCTATACGATGATTGATTCTGGAGCGAGAGGTTCTCAAACCCACCTTACGCAGATTTCAGGTATGAAGGGACTCGTAGTGAATCCACAGGGAGAAATTATTGAACTTCCGATTAAATCTTCTTTCGTAGAAGGATTGAAACCAATTGAATACTTTATTTCAGCTCACTCTGGTCGTAAAGGAAAGGCCGATACTGCCTTGAGAACTGCAGAATCTGGATACTTGACGAGAAAACTCTGTGATGCTTCCCAGGAAATGATCGTGAGAGAGGATGATTGTGGTTCTACAGAATCTATTCTCTATACAAGAGAGGAGGCTGAAATCAAAGGAGAAAAATTCTGAACTTTGATTTATGGTAGAGTGTTGGCTGAAGATGCGATTGATGAAAGAGGAACCGTGATATTGAGGGCAGGAGAAATGGTTACGAAATCAGCTTTAAGTCTGATTGAAACTTCAGGTATTACTGCTGTGAGAGTAAGGAGTCCATTGACTTGCCACTGCGTAAGTGGAGTGTGTCAGAAATGTTATGGAATGGACCTTGCTACGAGAAATATGGTAGAAGTAGGAGTGCCAGTTGGAGTAATCGCTGCGCAGTCTATTGGAGAACCATCTACTCAGCTTACGCTAGATACCTTCCATGAAGGAGGAGTGGCAGGTTCATCAGAGGCATCTGGTATTGATAGAGTGAAACAGCTCTTTGAGGTGAGACCACCAAAGAATCCTGCTGGAATTAGCCCATTTGATGGAACGATCTCTTTTGAAGAGACACCAGAGGGAGGAAAATTCGGAAAGATGAAGGTGACTTCTGAAATGCAAAAGAAAACTTACCTTGTGAAATCAGAGTATAAGGTTATTGTAAAAGAAGGAGCAGACCTTGTAAAAGGTGCTGTGTATGCGAGCAAATGAGCAAGTAAGCTTAAGATTAAGGAAGCTGGAAAAGTTCTTGAGGTGAATGAAGATTATATCGTGCTTGGAGTGCAGGAAACCTATACCAAGTCACTTGTTGGTTTGGCTCCAAAAAAGACTAAAGTTTGAGATAAAGTTTACAAGGGGGAGATCCTGACGACTGGGGCGCTTGATGTGATGGAATACAAGAAAATCGTTGGAGATATTCAGGCTCAGAGATATATTATTACTGAAGCTAAGAGAGTGTATGCTGCGCAGGGACAAGACCTTAACGATAAGCATATCGAAGTCGTAGTGAAACAGCTCTTCTCAAAAGTCTTTATTGAAGATGGAGGAGATTCAAGTTTCATCCCAGGGACTTATGTGAAGTATGAAGAATTTATCAAAGTGAACCGTGAATTGCAGGAGGCTGGAAAGAGAGTTGCACAAGGACAGAGGGTTGCTCTTGGACTGACGACGGTTGCCAAGGAAACCGATTCTTGGATGTCGGCGGCTTCTTTTCAAGAGACGATTAGAGTGATGGTCGGTGCTTCGCTTAGGGGTGCGATTGATCATCTTTCAGATCTCAAGGCAAACGTTATTATCGGTAGGTTATTGCCGGTTGGGGAGAACTATAGGAATATGTTTGGTTATTAATCCTTCTCACAAAAGAGAGAAACCTGATCGGTGTGGTCAGGTTTTTCGTTTGGAGAGGGGGAGGGCTTTATCGCTAAGAGATTGAGCATGGTTGGAGGGGGAAAATGCTTTTCTCGTTTGAGCAATTTTCTTTAGTCTCCCTTTTCCGCTTTGTGAGATTTAAGTCGAGCAGAGGAGGAATGTGGATTGCCACGCTTCGCTCGCAATGATGGGTATGAGGTCACTGGATTGCCACGCTTCCCTTCGGTCGCTCGCAATGATGGGAGGAGGAATGTGGATTGCTCTAGTTGCTTTGGTCGTTTGTGAGGAGAGGGATCGGGGAAGGAAATCAGATTTTTTATCTTTTTATTTTACAAGAAAGGAATGGGGAAGCAAAAATGGTATGTGATATGGCAAGGAACGAAAACGGGGGTCTTCTCCGAATGGAAGGAAGTGGAAGGATTCGTAGCGGGGGTCAAGGGGGCGAAATACAAGGCGTTCGCGACGAAGCAAGAAGCAGAAGTGGCGTTGGGGAAATGATGGGAAGCGTATTATGGAGCGGTCAAGAAGGTAGTCCAGGAGGCCATCCCGTTTTTTCAGCAGAGCATTGTCGTAGATGCGGCTTGCTCCTCGGCGACCAAAGTAATGGAATATCAAGGCATTGATTTAGCGACGGGGAAGCAAGTTTTTGCGGCTCGTTATGAGGAAGGGACGAATAATATTGGGGAGTTTTTGGCGATTGTGCATGGGCTCGCTTGGATTCAAAAAGAAAAGAAAGCTGACTATGTGCTTTATTCGGATTCCTTGATTGCGATGAAATGGGTCAATGAAGGGCGTTGCAAGACCTCGTTTATCGGGGCTGGGAATGGGAGCAGGATTTGGGAAGCGATCAAAAGAGCTGAGCAATGGTTGGCAGAGCATAAGTTTTCTACCCAGGTTTTGAAATGGCAAACAGAAGAATGGGGGGAGAATCCTGCGGACTATGGCAGGAAATAAAAGTGATTTTGGGTATAAAAAGGGGAAAGGGGCGAGAAATTAGCCCTTTTTTGCGATCTTCTCTTGCAATTAGGGCTGAAAAGTGCATAGTCAGGGTAGCAGTCTTTTTTTATTCTTTTTTTAACTACCAATGAAAAAAGTCAGATTTTTATGGTTTGGTGCGCTTTTGGTGCTGGCAGGAATGCTCTTTGCTGGGTGCGAGAAAGCTCAAGAAGAGAAGATTTCGATCAATACCAAGGATGCGATCAAGCTAGATCTTTTCCAATCTCAAGAGGAATGATCCGAAGAGGATTGGGGAGAGCCTGTTCCTAATACAGAAAAGCTGTTTCTTCAAGGGCAATTTGAAGAGCCGTATCGTGGATATTGCAGCAGAAGGGAGCTTCTTGTGAAGCAATGAAGATCGGTCGAAGAACAAGCGAGTGAAATAGAAGATTTGATTGCTCGCTATAAATGGATCAAGGTCTATGATATCCCTGAGGTAGCTGAGGATCAGGAATTGCTACAGTTTAATATCGCAAATTTGATGATCACCAAATATCCTTTGGTCATCAAGGACTATTACAAGCGTGGCAAGGAAGTGGGATTCATCAATCTCAAGACAGAAAAGCAAGGAAATGATTTGGTGATTCTTGGACCAAAGGTGAGCACGGGAGACAGCTATCTTTATGTAGATGTAAAGGAGCAAGGTATTGGTGGATTCAGATTTGAAAATAGTAATGAAGATCTGAAGCAAAGAAAGTCCTGGGAACATCCTCATTTTTTGAGGAGGGCAAGAGGAACATCTTTGTATAGTGGGCGTTGGTTGGTGAAGATATTGCAAGGGGGAGAGCGTTTTGACCGCGTCGTATTTCATTATCCATTAGGGGAGGTGCCACTTCAGCTCCCTGAATGGAATAATAATCCAGAGAACTGTGAAGTTGAGTTTAATGAGTATACGGTATTTACTGAATTGCCGCTCAGAGTCGTAGCTTCAGGGGTTGATTACAAGCTCGATTTTCATCCGGTGATTTCTGGAGCAGTGATAAAAAGTGCAAGGCTCCTCCAGCTTTCAGAAAAGCAGGCAAGTGGCTATCAATCTCTAGAAACACTCAAAGCATCGCTTGGTGAGAAGCAGGTCTTGATTGGAAAGCTTCCGTTTAATTACCAGGGATTTGCTGATAGAACGCAGAACGATCTAACTGATAAGACGGCATCAGATTTATTTCGTTTTAAGTTTTTTAAAGAGTGGACGGCTACGGTCAAAGCAGGCGTTCCTTCGCAAGTGGCGTTTACGATTCCTGAAGAAGGGAACTATCTTTTGCTCGTGGAGAATGATAATGGGCAGCTTTATGCTTTCAATTTTACGCCGAGAGCCGAGAATGCCCATGCTGTAGTCAAGAATGATAAAGAGGCAATGACGCTCACTATCGTGCATGATTGGCTTTTGACAGGGCAGGGGCTTTCTGGGCAGTTGGACCGCATCCTCGGAAGTGGGAACTATACGTTGGAGGTGAGTTCACTTAAAGAGGCGTGATTCCCTTCTAAGTATTGAGGTGTGTATAGAGAGGAGGGGAAAGAGTATTATCCTGAAGGGCAGTATGTGAAGATCTCCTATTTTATTGCTCCTAATAAGCACTACACGGGGAATTTGGAAATTTTGGATCTTGACGGCAAGAAGTATAGTCATGCGGTAGATTTTTTTGTGGAGAAGATTGGTGATGCGAGGGTTTCTCGTGATTTGTTTTCTCAGAAGTTGAATGTATTACCGAGTGAAGGCTATCGAGCGGACAAGATAAAAGTAGGCTCTAAAAATGCCAGAGAACTCAAGATCTATACCCAAGTTTGTGAGGTAAAGCAAGAGGGGGATTTTTCTGATGCGAAAAGGAAGGAACTGCTTCCGCCTCGTAAATCGCATAATGAGTATGATGATTATGATTCTAGGACTACCTTAGGAGAGTTGCACTTTTTCGATTGCAAGGGGAAGATAGAAGAACACACGGTGCCGGTAGAGAAGTTTGAGTATCGGAAGAGGATTGATCGTGAGCGAGAGCTTCCGGCAAGTGTGAAAACGGTGCCGTATTTCCGTATGAGTTTCGATAAAGGCATGAAAGATGCGAAGTATTATATGAGGAGTCCCGTTTGACTCTTTGCTAAGGTGGCGCCTTATCAGGAATTGTTGATCCGAGGGGTAAATTTTGCGGACGGGGCTGCGGTGCAGCAGGGAGAACTCCAGATTTTTGATGATAAGGGAAAACTGCTCAAGAAGGATCAGCTTTGAGCTAGTGGTTATGTTTTCTCGCTGAGTGGTGTAAGCTCTGAGTATTTCTTTGTTCGCTATCAGGATAAACAGAAGAAGGCTGACACGTTTCTTATTGCAACGGTAGATGGAAGGACAGACTTTAACGAAAAGCGTGCGATTAGTGCGTCTTGGCTGATGCCCGAACAGACTGATCAACAAATGGACTGGTATGAGAGGCGTGAGTCGCTTAAGATTTATGGTTATACAGACAGGGGGCTCTATAGACCTGGAGATACGGTGCATTTCGCCGGGTTTGTTCAGGATGTTGCGAAGGTGGCTAAGGATGGAACGAACGGAGTCCAGACAGGGTTTGTGATTGTTGAGATAACCGACTACGAAAGTGATGATAATAAGAAAATTGTTTTGTCCAAATTAGATGAATTTGGAGGGTTCGAGGGATCCTTTACCTTGAATGAGAGTAGCCCGCTTCGCTATTATCATATCAATTTTCATTATGCACTGGACAAGGATAAGATGAGCGTAGAAGATTTCGGTGGAGATTGGGAGGCTCGAGAGAATTATAGAAAGACGCATCCTGTGCTTTCTTATTGGTCAGATTTTCGTGTAGATGAGTTTCAAAAGCAGACCTTTAGTGTGCAAGTCAAAAAGGAGATCAAGGATAAGCAGCTTTATCTTGAATTGACGCCATCTTATTATATGGGAGGAGAATTAAAGGATTATGATCTGCATTTAACCTATTCCTTGGCGGCTAACCAATCATGCTCTGATTGTCGGTGGAGAGAAAGTAGCGATTACTACTATAACTATGTAGCGGATTCTTCTTTTTCTCGAGGCGGAAGCTATGAAAAGGAGCATCTCACTGGTGGTGCTGTTCTTTTCCAGATGGGGGATTTAGAGAAGCTCCCATATAAGGGAGAAAAGCTGAAGCTCCAAGTGCAGGCTTATGTTAAAGATAATCTTTCTGATGAGACCCGTATGAGTGTAGAGTATTTAGATATTGATCCAGAAGTGAAAATTGGGCTTAATGGCTATGCGTATGATTGGATTGATAGTAGTAGCTTGAAAAATTATCGTCTGACCTCAAGAATAGAGAGCAATCTCAATAAGGTTGCTGAACTTAGCTATGAATGGTATTTTAGACCGTTTGGGTTTGAGCAATCGGTTGATACGGAGGGAACTTTCTATTATATCAATGGAAGTAAGCTTCAGCTCGTTCAGTCTGGAACTTTACAACCAAAATCGGAAGTCAGCATTCCTTTGCTTTTTGAGCGCTATCAAGGAGGTGAGTATTTCCTTAGAGTTATTTCCAAGGACAAGTATGGCGTAGTCAATGGAGAAGTAGATAAGAATATCTATTACTACGATAGCAAGTTTGATAAAGGATTCCAAGGAGATATGGAGAATAACTATGCTCTTCATGTGCAGATTCCAAAGAAGAATTATGAGATTGGAGAAAACATTCCGATCAGTATCAATCCTTATATCAAATGAGCAACGGCTTTGCTTACGGTAGAGAGAGGAAATCAGATCTTGGAGAAAAGGCTTGTTCGCCTAGATGGAAAGCCACTTACCTTGACTGCGAAAGAGGAGTTTTATCCTAATGTGCAGGTGAGTGTTACGGAGTTTGTAGGGGAGCAGGTTAATAGTCAGCTTGGAGGTAGTGGAGAACAAAAGAGAAAGGAGCCAGCTTTTTTTGCCTGATATGCGGAAGCGCTCATTCAGGAGAAGCTTAAGGACATTACTATCAAGATGGAGACGGATAAAGCAGAATATCAGCCTTGAGAGAAGGTGAAACTCAAGATTTTTACTACGGATTATCAAGGTAAGCCACTTACGGCGAGGGTTTCGCTGAGTGTCGTAGATAAAGCTTTGAGCGATCTTTATGATCTGATTAAGGATCCGATTCCTTACTTCTATAATAGAATTGGTAGCAGCATTGGGAACTTTGGAAACTGGAAGAACCTCTACTATGCCTTGAGGACCTTCTTTGCTGATGGAGAGAAAGGTGGATGAGGAATGGATGGTTCTTTGAAGAATTTGAGGAAGAAGTTTTATGATCTTGCATTCCGAGATGCTGCGATTATTACTACGGGGGGAAAGGCTGAGCTTAGCTTTACCCTACCGGATAATCTCACGACTTGGATGCTCGATGCTGTTGCGGTTTCTCGTCAGAGTCAGCTCGGGACTTCAAGAGCTTACTTCAAAGTGAATCAACCTTTGGTAGTGGAGGCGAATTTGCCGACTTTCCTTACCATTGGAGATCAAATTACGCTTCCACTCAAACTGATTGCTTCTCCAGCAAAGCTTGCTCCATGAACAGAAGCGATCCTCAAAGGTGTCGTAAAAGATGAAGCGTGAACGGTGCTCCAGTCTTTTGAGCAGAAGGGGAAAGTCAATGAAAAATTCTGAATCCAGATTTCAGTTGGTGAACAGTTCTTCGATCAGCAGTGGGTGACGATTGAAGTCGAAGCCAAAGCAGGAAACTTTCACGATGCTGCTCAGTGGAAGATCCCGGTAAGGACTGAAGGGCTCGTAAATAAGAGCTTTTCACTTGAACAGCAGAGGAGCGGTGAACGTTTATTTACCTTTGATGATGAGGTGGTGAGGGGAAAACTCGTCGCTCGTCTGGCTCCGTTCCCAAGTTCTGTCTTGGCAGAACCGCTTCACTATTTACTCTTCTACTCTCCAAAGAGTCCTTCTGAGTGGCTCGTTAAGACGGCTCAAACTGCACTTGAGGCAGAAAGCTTGACTAAAGCAGGACTCTTGACCGGAGATTTCGTGCAGAGTGGAGCTATTCGTCTCGCAGAAGGTAAAAAGATGGCAATCTCTGAACTTTTAACGGCGGTGCTTTTCCAGCTCAAGGGCAGACAAGACTGGGATGGTGGAATCAAAGAACAAGATAGAAGTGATTATCCGTCTCGTTTTTGGGAGCAGAAGAATACTTCTTCACTCTTGACGAGTATCTCAACCTATGCCTTACTCGAGACGATGAAGACCAATGGATATGCCAACCTTGTGCCAAAAGATTTCTCAGAGAGGCTAGAGCGTTATCTTGCGAAGCTTGGAAGTGATTCCCCAGAACTTTATCTTTACTATCTCGCTCAGAGAAAGACTAACCTCGCATCAATCAATACCGCACTCGTTTCACAACTTCAGAAAAAGTATCCAAATAATATCGCTATTCAGGCTTTAAGCTTTTATCTGCTTGCACAGAATGCTGGAGAGGCAGATGCGATTCGCTTAGCTCAGAGTGTGGAGAAGCTTTTTGAGCAGGCGTTTGCTCAGGGACGCTATTTTGAAGGGGTGCCACTCACTTCGCTTGAGGAATTAGCTCCTGAATTGCTTGCTTTGGCAGAGGTAGCCGGCAATCGTAATGCTCATTTATCGCTAGATCTCAAAGCTTTACAAGGGTACTATCTGAGAGGAGTTCTCAAACTCCATACTAGATTCCCAAAGCAAGAGCATTTTACGAACCAGATCTCGAGTCAGATTCTTTCTCTTCTCAAGTCCAGAAGTGCGTATGGGCTCTGGAGCTGGTCGTCTACGACAAACTTTGCTGTCTTGAGTGCTCTCAATGCTGCACTCACCTCTTACTATCAGCATCAAAGTCAGACAACTCATTGTGTGCTCCAGGTGGGGAATACGAAGCTCGAGCTTGATGCGGGGAAGACTTGTGTAGAAAAGAGTATTCCGTTTGAGCATACCAAGGATCTGAAGGTGCAACGGTCCTGTGAAGGTCCTGCCTTCCTTGATCTCCAAGTCAACTCTTTACTCAAAGATATTACCAAGAAGAAGACCGAGCTTCACAATGTGGAAAAACTCACGTTTGAGTATCCGACAACGGCTCAAATCGGTGAAAAGATTCCTCTTAAGGCGGGATTCAAAACGCTCAAAGAGGCTCAGAATTTGACTGTTGAGTTTACGATCCCTTCCAGATTTAAATTCTGGTCCAATCTCCAAGTGGCAGAGAATAGCTTTGAGAAGGGAATCTTTGACTTTGGAAATGATCGAGCGTGCTTTCCGGACTTTTATGAGTTTAGGTTTGATCGTGTAATCCTTAACTATGGCACGATGAAGGCTGGTAAGGAATGTAACTTTACCTTTTATGCGGTGAATGCGTTTGATGGGCAGTTCGTCTACCCATCGAGCAAGCTTTATGAACAGCAGGCTACACAAGTATGGTGAAGTAGTAAAGTAATCAGGAAATAAATTCTGAATCTCTCCTCGTTCGGCTGGGAAGTCTTCGGTTGGGCTTTTCTCGGCTGGGTGAGGAGGGGAAATGGAGCCGTTCTTTAGTGAGCAAAAGAGATGAAGTAGTGTTTTATCTTATTTATGTAGAAAATATGAAGAACAAATGGAAATGGATGATGGTTGCTGGTCTTTTGCTTGTGGTGGCTGGAGGTATTTTCTTTTGGATTCGTAGTGATCGTGCAGCTACGAGGCGTGAAATGATCCTAAAAGATAGAGCATGTCTCCTAGGCTATACCAAGTTTCTCCATTGTTTTATAGGGGATACTTTGCAGGGAACTCAGGAGTGTAAGGACTCTTTCACTCCAGAAGAAGAAGCTTTTGTAACTCAATGTTCTTCTTACTTTATACAGAACTATAAATTGAGTAAATGTTTTCGTAAGGGGGATAAGAAATTAGCATCTCTGGCTGAACTTCCTTCAGCAGATCAGTATTCAACCTTGTATACCTACGGATGTATGCTCTCTACCTTGTATGATTTTTAGGTTCTCGGTTCAAGCTGTTTACATTCTAGGTTTTGCAACTCGGGCTCTCTCGCTTTTTGTGAGGATTGGCTCGGGTTGCTTTTTTTTTAGAGGTTTGGCATGATGAGGCGTGGGGGGAGGCACACTCAGCAAGCAGTCGACATCCTGTGCAACTCCTTCTGGTATGTGTTGCAAACCCTTGTTAGCGTAGCTGGATTTAGGTTTGCAGGGCTTGCAAGTGGTCGGAATGGAGCATTTTTGGGGTTTGCAAGACGTGCAAGTGGTCGGAAAGGATCATTTTTGGGGTTTGCAAGACGTGCAAGTGGTCAGAATGGAGCATTTTTGGGGCTTGCATGAGCTGCAAGTGAAGAAAAAGGGGTCTTTTTTGCTGTTGCACGGTGTGCAAACCTCCTGCGAGGGTCCTTTTTGATTGTTTCACGGGCTGCAAACTACTAGCGAGGGTCCTTTTTCACTCTTTGCACGAGCTGCAAACTACTAGCGAGGGGTCTTTTTGATTGTTTCACGGTGTGCAAACTCCCAGCAAGGGTCCTTTTTCACTCTTTGCACGAGCTGCAAGCTCTCTGCGAGAGTCCTTTTTTGCTCTTTGTAAAGGGTATAAAAGGTTATAATTAACCATTGAGAGAGGTACTTGTTCGTTCTGAGTGATATTTAGGTAAGAAAGGTGTGATTTTGGAGCACTGTCCTCTTGTATTTCTATAAGAAGTTCTTAAAATGAGTCTTGTTATAATAAAAATTTAAAAAAATTGAAGTGCTTTTCTCTGTGAGAAAGGAGCTTCTGGAGTTAGAAGAGGGACTCTATGACTCCTACTCTTCAACGAAGTGGACGGACTTCATTTGAGGGGTAGATTTCCCCTCCCCCCTTTCTTTTTTATCTGATTTTCTTATTTCTCATGAAAAGGCATGTATGGTATTGTCTGGCTCTTGTTCTCCTTCTCGGGGCTCTTGGGGTTGTATCCTCGTTTTCTGTAGTCAGGGAAACGGTCGTCTGAAAATTCCTCTGATATTGGTTTATTCTTCATAACGAACATACTCCTTCTCAGCATTCAGAGGTGATTTCACAAGTTATCTGGCAAAAAAAGGCGAAGTATGGAGATCAAGTGATTATTTTACCTCAAGAAGGAGAGATTCCTTTGGATGAGTACATTAGAGCATCACAACAAGCTCGTGTGCAAGAAGGAAGACTCCCGAGTTTTATGATGGAATATCCTCGTTTTTCTTCCCTTCTTCGTGATGCAGGGTTTGTGATTGAGGCGGTTTCTGGAGGGATTCCCTTCTGACTTCGGGCGTATTTACAAGGGGATGATATGATCGAGTTGCCGTCTTTCCCGATTGAGACGAAGCCGTATGCAATCTATGCTGAGGGAGAGAGAGAAGGTGTTGGAGGAGAAGGGTTCCGTTATCCGTGTGTGGCGTATCTTAGAAGTGCGACTATGAACTCTGGGGTAGATCCTCGTTGTTTACAGGTTCTGATTAATAGGCAGGGGGACGATCTTCGTCTGTATTTCGATGCGTTTACGGGAACGGTGCTCAAAGTAAATCCAGTGGCTTCTCCGTTAGAGCATTAGTATAGAGTGTTTTTAGCAAGATGACTAGCAAGTGTTTATCCCATGGGATAGGCGGTTGCTGGTCATTTTTTTTGATGGTCCTTTTAGAATCTAACCTCTGGAAAGAGAGGGGATTTTTGATTTTGGGCTTGAAGATGAAGGCTCAATCCTTACCCTTTGGGAAGTTTCCTCTTTGTAATGAAAGGGGGGTTTAGTTGTTGGGGTGTTGGTCATGCTACTGAAATGGTGAAAAAAAGTCTGATTTTTCTTTCTGGTTGTTATTGGGATTTGGGTGCTGGGGAATTTTTGCTATAGGGTGCCGCTTCCAGAGCTTGAGAGGCAGGTCCTCAGAGGGATCAACGGGGAGCTTCTCCAAGGGCTGAGGGAGATTCCGTATGCGAGTCCTGAAGAAGTGCCTGAAAATCTCAAGCGTGCGGTCGTGCGTGTGGAAGATCGTAGGTTTTATTGGCATCTAGGGGTTGATCCGATTGCGATTTTGAGGGCGATGCGGTTCAATTTGACGAGTATTGGGAAGGTGCAAGGGGCGTCCACCTTGCCTCAGCAACTTGTGAAACTCACTGAAAAGGCGTATCACAGGAGCTGGAAGCAGAAGCTTTGGGAGATGGGGCTTGCGTTCAATCTCTCTTTACATTATTCCAAGCAGGAGCTCCTCTTGGCGTATCTGAATCAGGTAGAGTTTCTCAATGGCGTGCGTGGCTACAGGTCTGCGTGTTTGAGTTATTTTGGGAAGGAATGTGGAGCGCTGTTTCCTTCAGAGCTGAGTTTTTTGATTGCAACGGCTCAGACAGGGAAGAATCCCTTGAGGCAAGGGGATTTTCAGCTCATAAAAGAGAAAGCTGAAGCTCTCTGTCAGGTGGCCTTTAGCAAAGAGCAATGCGATAGATGGGAAGAGCTTCCTCCGCTATCAGCAAAAGCGTTGCTTCTCCAGCCTAACCGTTCGGTTTTTCAGTTTTCTGACTATTATTTATGAGGGTCCTGAGCGAAGGGACAGGATTCTTCGTTTGATGTTCCTTTGTATGAGCGTATTGAGCAGGTGATTTCCTCTAGTGAGCATTATAGGCAGCAGATGCAGATGGAGGATTGTTGTGTATTGGTATTGGATGCGGCGGGTGAGGTGCGTAGCATGAATACGTGTAGACCGTTTTATGAGAAAGAGCAAGGGATGGTCAATGGCTGTCTGAGTAAGAGGCAAACCGGATCTGCGATTAAGCCGTTCCTTTATTTGTTTGCGATGAATGCATTGCAGTATACGGGAGGGACGAAGATTGTGGATGAGCCGGTCTCTTACTATTTAGATGAAGAGCATATTTATAGTCCGAAGAATTTTTCGCTTTCCTATCATGGAGAAGTATCGCTTGCAGATGCGTTGGGGAATAGTTTTAATGTCCCTGCGGTGAAGCTCTTGCACGAAGTCGGGATTGAGGAGTTTATTGCATTTATCAATCAGCTGAGGCTCCGTTTCTGAGGAACCACACAAGAAGTTATCACTGAAGAAGGGAAGGTCTTTAACGCTCAGCATTTGGGGCTTTCGGCGGCGCTTGGAACCTATGAGCTTTCTCCGTTAGAGTTTGCACAGTTTTGGAGGGTTTTCTTGGCGGGAGCTGAGGAGGGGCTTGTTGCTTCCAAGGTTATTGATGAGGTATTTGCTATTTTGTCTGAGAATCGTCATAGGGTGGTCAGTTTTTGAATTGATAACTATTTGGATGTGGCGTGATGGGCAGTTAAATCAGGCACTTCTAGGCATTTCGTCGATGGTTGGACCTGTGGTGCGAATCGTGAGAAGCAGCGTATTGTCTGTGTATGGGCGGGAAACTATGATGCTACTCCTATGAAGGAGAGTGGAAGTGTGACTGCAGGTTATTTGTGGAATTTGGTTGTGCGTATGCTTTAGCTGTGTGGTTTGAGTAGGGGAGGGATATGGGAGCGCTTGGAGCAGGCTGTATATTCTCCTCTTGAAATCAAAGCTCTAATGCTTATCTTTGCTTTGTTTATTCTTGATAACTAGGGATGTGAGGATTTTTGGGGAGGTTTAGTTTTTCGGTCTGTCTCGGAGAGTAGTTTCAACAGAGGAAGTCTGATTTTTTAACTTTTATTGATACAAGCGATGTTAGAGAAGTATAGTCCAAATGAGATAGAAAACAAGCGAGGAAAACTTTGGGAAGAAAAAGGTTATTTTAGAGCGACAACTGATGCTGAGAAGGCGAATTTCAGTATTGTGATTCCTCCTCCAAATGTGACTGGAATCCTCCATATGGGACATGTGCTGGATGAAGCGATCCAAGATGTATTGGTGAGGTGGAAAAGAATGTCTGGATTCAATACTCTACGAATGCCAGGAACCGATCACGCAGGAATCGCTACTCAAAACAAGGTAGAAAGAGCTTTAGCTGAAGAAGGGAAGACAAAAGAAGACCTTGGAAGAGATGAATTTATCAAAAGAACTTGGGAGCGAAAAGAAAAACACGGTGGGATCATCACTCAACAACAGAGAAAACTTGGAGCATCACTCGATTGGGAAAAGGAAAGATTCACGATGGATGAAGGGCTTTCTGCGGCAGTGAGAGAGATTTTCGTGAAACTTTACCATGATAATCTGATTTATCAGGGGGAATATATGGTAAACCGATGTCCAAGGTGCGGAACTGCACTTGCTGATGATGAGGTGGAACATGTGGATTCTGAAGGGCATTTTTGGGAGATTGCCTATCCGATCGTGGGAACTGATGAAAAGCTGATCCTTGCGACCACGAGACCTGAAACGATGCTCGGAGATACGGGAGTTGCAGTGCATCCTGAGGATGAAAGGTATCAACATTTGATCGGGAAGAAAGTGCTTTTGCCACTGATGGATAGAGAAATCCCTATCGTTGCCGATGAATATGTAGATAGAGAGTTTGGAACTGGAGTGGTGAAGATGACTCCTGCTCATGATCCAAATGATTTTGAGGTGGGAAAGAGGACGGGACTGGAAATTATCAACATTTTCACTCCTGATGGAAAAGTAAATGAAAACTGATGAAAATACGCAGGTTTGGATCGTTTTGAGGCGAGAAAACAGATCGTAGCTGATTTGGAGGCTGGGGGGTATCTCGTGCTAGTGAGGAAGCATCAGCATGCCGTTGGGCATTGTTATAGGTGTGATACCATTGTAGAACCTCGCGTTTCTAAGCAGTGGTTTGTAAAGATGAAACCTCTCGCAGAACCAGCACTTGAAGCGGTAAAATCTGGAAAAATCAAAATTCAGCCTGAAAGATGGGTGAAAGTGTATTATCATTGGCTGGAAAATATCAGAGATTGGTGTATTTCTAGGCAGATTTGGCGAGGGCACAGAATCCCTGCTTGGTATGGACCTGATGGGAAGGTTTTCGTGGCAAAAAATGAATCTGAAGCGAAAAGTCAGGCTTTAGCTTTTTATGGGAAAGAAGTGAGTTTGGAGCAGGAAAATGATGTGCTTGATACTCGGTTTTCTTCTGCACTTTGGCCATTTTCAACGATGGGACGACCAGAAAAAACTGAACTTTTGGAACATTTCTATCCGACTTCAACCCTCGTAACGGGAGCAGACATCATCTTTTTTCGAGTAGCAAGAATGGTAATGTTTGGACTGTATGAGATGAAAGATGTGCCGTTTCACGATGTGTTTTTCCATGGAATCGTGAGAGATGAGCTGGGAAGGAAAATGTCCAAATCGCTTGGAAATTCGCCTGATCCTTTGAAACTCATTGATGAATATGGAGCAGATGCGATCAGATTTACGATGATTTACAATACTTCCGCTGGTCAGGATGTGCATTTCTCTGAAAAGCTCATTGAAATGGGGAGAAACTTTGCAAATAAGGTTTGGAATGCGACCAATTTCGTTTTGATGAATTTGGAAGGCTTTGATCCAAAACAGGTGAAAAAATCAGATTTGGCTTTTGAATTGGTAGATCAGTGGATTTATTCAAGACTGAATACTGCGATTGCGGAGACGGAAAGCTATTTGAATAAATATGCTCTTGATGAGGCGGCGAAAGTCGTATATGAGTTTATCAGGGGAGATTTCTGCGATTGGTATGTAGAAATGGCAAAGGTGAGGCTCTATATGAATGATGACATTCAGAGTAAGCAAACTGCACAATGGGTGCTTTGGGAGGTGCTAGAAACCGCTTTGAAACTGCTTCATCCGTTTATGCCGTTCATCACTGAGGAGCTGTGGCAACATGTGAAAACTGAGGGAGAAACGATTATGCTCCAGTCTTTTCCAAAAAGTGAAAAAAATCTGATTTCTGCTGAGGTGGAAACTGCGATGGCTTGGCTCCAAGAGGCGATTACTGCCTTTAGAAATATCAGAGCTGAAGCGAATATCAATCCATGAAAAGCCGTTCCTGCTTTGATCAAAACTGCCGATCCAGAGGAAATGAAGATTTTGACTCAAAATAAAGCCTTTTTGATGAAATTGGCGAAATTGGAAAGCTTGGACTTTGGTGCAGAAATCGTGAAACCTGAACTTTCAGGAGTAAGGGTCGTTGGAAAGTCAGAATTGATCGTGCCTTTGCTTGGATTGCTTGATCTGGAAGCAGAAAAGGAAAAGATTATGGCTCAGATTGCCAAGCTTGAACAGGGATTAGCTTCTTGTGAGAGGAAATTGGCTGATGAGAGGTTTGTTTCCAAGGCTCCTGCACAAGTGATTGAGAGAGAAAAGGCAAATCAAGCAGATTATCAGCATAAGATTGAAAAGTTGAAAGAGAATTTAGCTCATTTAGGTGGTTCTATTTAGCTTTTTTGGTGTGTCTTTTAGGAAGGATCCCGATAGGGATTTTTTCTCTATTGAAAAAACATCGTATGTATGGTTGATTATTCTCTATTCAACTTCTTTAATTAGAATTGTTAATGTGAGATTACGGTAAAAATAAAGCATCCTTTATGATTTCACTTTCCCTATCACGAATTATTATGGTATTGCGGTTTATCTTATCTCCACTATAGTACATGATGTATTTATTGTTCATATAATATATTGAATATATGTTGTTTCCAATTTGGATATTTGATGTGGTTTGTTTGATAATAAGAGTTCCTATTAAGGCAGATAGAAACCAAGTAATTCGTAATATTATTTTTCTTATTTCCCTCGTGCAGTTAAACTTGTTTTTTAACGATTCATCTACCCGAGAAAGAATGGCAATGAATATTATAACTGTACATGTAAAATATCAAGCTATGATCATGCTGTCATTAATCCATGTAGATGCATCAAAATAAGGATAAAAAGGAAGTTTAATCGCTAAAAAATGTATAAACTGGTATAAAAATCCCAATATCCCTCATCAAAGTGTTAGTAAAATTAGTATGTCTGGTAGAGAAAATTTATTTTTATACATATATTTTTGAATTGCTATTTTACTTTCTTTTCGTTTAAAATATTCTCTGAGAAAGTATATACCTCAAAGAATTATTATCAAAAGTGAAAAAATTTGTCGTATGATATTAAGAATTTCCATAATTTTAATTTTAATATAAAGATTTTATGTAGTTGGGCTTTGTTTTTTATGTATTCAAGTTTTTTAGTGTGAACTTATTGATTGATGTAGGAATATTTTGATAATATTCTTTTAAATAATTAGTAATTTTGAATAGATTTTCAAATAAAAGTCTGATTTTCTTGCAACTCCTTCTCAAAACTGTAGACTGATAGAACTTTATTATCTTGTAAAATAAAGATGGTTGCTTTTCAGATTTTTTGATTTTCTGTGTATTGGTATGGGATTTTTTATGCGGTCGCGTTTCTTTTGTGATACTTTTGACTGGCATGGATAGGAAAGAAAAGATGGTTTTCAAATCTTCCTCAGGTTCAATATTTTCTGACTGAAGGTTTAGAAAATCTGATTTTTGCTATTGCTATGGGGGTTTTGGTGGGAGGAAGACTTGGGCATGTGTTGATCTATGGAGAAGGCTATTATTTCGCTCATTTAACGGAGATTTTTAAGGTTTGGGAAGGTGGAATGTCCTTTATTGGAGGGATTTTCGGGGTCGTGATCAGTATTTTTTTGCTTTGTCTGATGAAAAGAGTTTCCAAGAAGGAATTTTTGGTTCTTTTTGATTTGATGCTTATTTTCGTTCCTTTGGGGATTTTTTTTGGGAGGTTTGGGAATTTTCTTAATCAAGAATTGTATGGAATCCCGCTTGCGGAGCTTCCTGATCGATTAGGTCAGTTTTTTTCTTCTTTAGGGCTTTGACACTACTATCCTAAAGTTGATGGTTTGCTTAGGGTGAATACGAACTTTATTTCTATGGCACTGGAGGGAATTTTGCTGTTTATGATTCAGGTTTTGGTCTTTTTTGGTATGATTAGGAAAAAAGAACGAAAAGTCTGAAGCTTAGCGACGAATTTTTTGCTTTTTTATTCGTTGATCAGGTTTTGACTGGAATATCTCAGAGCAGATAGTCAGCTGGAATTTATAGGTTTTTTTACCAAAAGTCAGCGGTTTTTCCTTGGGTTTATGGGTATTGCACTGGTGCTTAAGTGGTTTCTTCGTAGGGAACAGCATTTATCTCTTTTCAAAGCTTAATGTTTAGTATCACTCTTAAATTTTTGGAAGCGGTGAAGCGTAATAACTCGACAGAGTGGTTGCATGCTCATCGGGATCTCTATCAGCAGGAAAAGAAGAGATTTTCTCAGTTTGTAGAGGCTTTTTTGAGGGAAGCAAAAAGTATGCATCCGATATTGGAGGATTTACAGGTCAAGGATTGTATTTATAGGTTTAATAGGGATTTGCGTTTCTCTAAAGATGGGAAGCCGTATAAGGAGAACTTTGGAGCGATCTTCGCTTACTGAGGAAAAAAGTCAGATTACCCTTGTTTTTATTTCCATTTAGAGCCGGGAGCGAGTTTTTTGGGTGGAGGAGTCTATATGCCAACGTCAAGCTCAGAATCGAACGTCAGGAAGTATCTTTTGAGGAATTATCATAGACGAGAAAAGCTTTCTTGAGCTCCTACATTTAGTAAATTTTATCGTCTCTGGGATGCTCCTCATCATTACAAAACGACTTGGAAATTGAAACAGCTCATCAAAACTGACCCGAAGCTTCTTTCAGAGTTAAGTCCTGCTTTGAAAAAGTATTGCCTTTTGGATGAGGCGAATCTTTCACAATCTCCAAGATCTTCATCGTCAAGTGAGCAAGTTTTGAAGCAAGTCGCTTATTTTAAGGACTGGATTTTTGAGCATTCGCTTTCTGATGTTGAGCTTTGTGAGCAAGAGCTTTTGTGAAGGTTTGTTGAGGCTTATCGCTTGATACTTCCTTTGTTGGAATTTTTGGAAGAAGCGTATTCACTTTAGATGTATTATGGTGTTTGTATGCAGAGTCTTGTTTTCTCCTGAGCTCCAGAGAGAATTGATACGTGGTTAGTAAAGCAGTTTCCTTATTCTAGGAGCTTTTTTCATCATATTATTGAGAGAAAGGGGATTTTTCTCAATGGGAAACCCGCAAAGAAATCAGTAAAACTGAAAGTAGGTGATCAGATTGAAATTGATAACCTAGAGAGATATTTGTCTCCTGTGATTCTTGAGGAAGCACCTTCTATTGAGATTCCGATCGTGAGGGAAACCACTGATTATCTGGTTATTAATAAACCGAAATGAGTTTTGTCCCATCCCAACAGTATTTGGGAGGTTGGCAAGCCGAGTGTCGTTGGCTTTCTTTATCATCATTACAAAGAGCTTCCAAGCATTGGGAATTTTATCAGAGCTGGACTGCTGCATAGGCTGGATAAGGATACTGATGGACTCATGATTATTGCAAAGACTGAAGTCTGACTTCAGCATTTTAAAGCTCTTTTTAAAGCGAAATCTGAATCTGAAACCCTTGAGCAAAAAGACAAAGTTCCGCTCAAAAAGCGATATCGTGCAATTTGTGAAATTACGCCTGAGGGAAAGCCATTTTTGGATGAGATTGAGGGGAAATTGCCTTTTATTATCAGCGAGGAAGTGAGAGCGAAAGTCCCTAATGCGGTTCCAAAGCTCGGAATCACGCAGATTGAAAAAATAGAAAAAAAACCTGATGGGAGGATTCAGCTTTTTCTCCAGATTTTTACTGGGAGGACGCATCAGATCAGATTTCATCTGAGTAATCACGGTTTGCCGATTGTTGGGGATTATCTCTATGGGAAGGAAAGTCAGGAAGCGATGCAACTTACCGCTTATCAGCTGGAATTTGAGGATTTAGGAGGAGAGAGGGTAACAATAAAAATTGATTAGTTTTCACTTGATAAAACTAATTTTTTTCCTATTCTTAAGTCAGATTTTTATCTTCTTTTTGCTTGCTGATGAATGAACAGGTTTTTGAGCAGATCAAGAGGGTTAATGAATACTGAAAAGAGTATTGGCATGCTAGAGAGCTTGCGAAGGCTTTGGAATATTCTGATTTTACTAACTTTTCCAAAGTGATCAAAAAGGCGGAAATTGCCTGTAAAAAAAGTGGTCAGGCTACGACAAATCACTTCAGCGAATTTACGGAAAAAGCAAAAATTGGTTCTGGTGCGACGAGGGATTTAAAAAGCTGGAAATTGAGTCGTTATGCTTGCTATCTCATTATTCAGAATGCAGATCCGAATAAGGAAATAGTAGCTCTTGGGCAAAGTTATTTTGCATTGCAGACAAGGCAGCAGGAGCTTTCTGCTCAGTATCTCGAGGATCAGAAAAGGCTTGAGCTCAGAGAGGAGGTCACCAAGCACAATAAGAAGCTTTTTAGCACGGCGAGGGGAGCTGGAGTTTATGACTATGCGACTTTTTATGATTCTGGGTATCAAGGGCTTTATGGGCTCAAAAAGAAAGAAATTATAGAAAAGAAAAATCTGAATCCAGATGAGAATATGCTTGACCATATGAGTAGTGAGGAATTGGCTGCGAATCTTTTCCGTGCGACTCAAGCTGAAGCAAAGATTAAAAGAGAAGCCATCAGAGGACAAGAAAAGGCGAGTCAAGCTCATTTTCAAGTAGGACAAAAAATCAGAGCCACCATCAAAGAGCTCTGAGGAACGATGCCGGAAGATATTCCAGCGGTGGAAGACATCAAATTTGCGAGGAAAAGGTTAGAGGAGCTAGAAGTCCAAGATGCACAGCAGATGCCGAGTTTCGCTCAGCAGCTTGGTGAAAAGTTAGAAGAGCAAGAAATGGTCAAGTATGAGAAATTGCCCGATAATATTCAGCTTTTGGAAGCTTTGGCAAAGATTATCAGAGGTTATCCTGGAGAGAGACTTCTGCTTTTGGGAGAGAAGAAATTTAAGCTTTCCAGCGAGGGGATGAGATTGGTGAAGGGGCTTTTAAATGGATAGTATTTAAGTCTGACTTGAAAATAACAGGCAAATTCCTATCTTTGAGTCAGTTTTTAATTTTTGATAGAGAAACAATGGTAAAAATTGATGCGGCAGAAGTAAAGAAAGGAACTGTGCTTGAGATGGATGGACAGCTTTTTAAGGTGCTTGATCTTTCTTTTATGCAGATGCAGCAAAGGCAAGGGTCTTATACTTATAAGGTAAGAAATCTGATGACTAATGGAGTACAAAATGTGACTGTAAAGTCCGGAACTACCATGGATCAGGCAGATATTTCTACTCAAAATGCAGTTTATCTCTATAATACAGGAGATACTTATTCTTTCATGGAGAACGATACTGGAGAGATTCATGAAATTGCAGCAGATACGATTGAAGATATTACCGGATATCTAAAAGATAATCTTGATGTCTTTATTCAGATCTACAAAGGGAATGTTATCAATGTGATCCTTCCTTCTACGATCAGCTATACGATTACTGAAACCGTTCCTGGAATTAAGGGGAATAGAGCTCAGTCTGGAACTAAGCCTGCTACCATTGAAACTGGAATGGAAATTCAGGTACCACTTCACAAAGAAGTAGGAGATACCGTGACTTTGAATACGCTTACTGGAGGTGTAAACTAGTTTTTGGATTACTATTTTGAGAAGTGGAGATTCAGTTTATTTCTTTTGGTGTGTGGGATTAGAATGGCAAAAATTCTCTTTGTCGTTGCGAATGTAGGGTTTCAGGATGAAGAATTTAGTATCCCTTTTGAACTCTTATCTTCTCAAGGGCATTTTTGTGACGTTGTTTCTTGAAAAGGTGGGAAATGTAGAGGCGTCTTTTTCAAAACCATAGAAAAAAGTCTGAAATTTGAGGATGTTCAGGTTTCTGACTATGCTATTGTGGTGTTTGTTGGTTGAGGTGGTGCTTATGAGGAGTATTTTCAGAATGCGAGCTATCTTGACCTTGCTCGTCAGGCGAAAGCGATTGCTGCGATTTGTATTGCTCCAACTTTACTTTCTGATTCTGGTCTTTTACAGGGGAAACACGTTACCGGTTGGGATGATGGTTTCTGAACGCAGATCGGTTATTTACAGCATAATGGGGCTATTTTTAAAGATGAAGAAGTAGTGCAAGATGGAAATCTGATTACTGCGAATGGTCCAGCTGCCGCGACGAAATTTGCTTGGGTGATTGTTGATTTTCTTAAAAATTCTTGAACAGGAATTTATTCCTCATGAGAATATCATGAGTGATAGCCCGTAGGAACTGGTAAAATATTTCCTTCTTCATCTATTTCGGTGCGATGGGTTTCAAGATATTCAATTTCTCTTTTTTGAGCTTGCTGTTTTTGTTTATTTCTTTCTTCCTCTAAAAATTGTACTTTAAAACATTCTTTAAGATAGCTATCTAATTCTTCTAAGATCGTCTTTATTTCTTGATTATTAAGTTTTTCTCGAGGATCAATCAGATTTTTGAACTGTGAAAAAAAGTTCTCTTCAAAGGATTGAAGCGGATTTTGAAGGTTTCTTGGATCTCAATACAACTCTCTAATATTGTCTCCTTTTTCATAAAGTTCTCTAGTGCTATTTCCCTGTTCATACGAGTTTTCTTGTTCTTGTTCGGCAAAAAGCTTATCCCTGATTTGTCAGTGGCTAAAGATTAAATTGAAAAGTGCTTCCGGTCAAAGCTTATGTAAAATTACTTTTGTTGGTTTTGTGATGTGTGAGCTATGTTTTTTTAGCTGTTCGAGGTCTTCTTGTCCAAGAGCATAAAGCGAACTGCACTTCTGATTCAGCTTTCCCTGTAAGTTTTCAATGAGTTTTTGCTGGGTTTGCTTTTCTTGTTCTTTTCTCTGCTGAGAAATAAAGGAGCTATATTTGTTTAAAAGTGGCTTTAAATGGGATTCTGGATCGATGATAGGCTCTCCGAAGGTATTGCGAAGAGATTTTTCCCGTTTGGTAGTTAGGAATTTAAGAGCATCGTGATAACCTGTCCTGAAATTTCATGAGCTCTTCCAAATTTTTCTCGCTTCTTCGAGTGAAGGCAAAAATTCATTGAGAAAAAATTCTTTTTCATTCTGATTTTTTGCAAGTATTTTAAGTATTTTTTCTAGTGTTTGTTTTTCTTTTTCTAGATAGGTAGAAAATTTTTCGTAGGAGAAAGTCGAGGTTTTATGTTCAGGATATTCTCTGCTATTCATGTCTATTTAGGCAAGGGTAAAATACCCTTTAAGTATAGGTATTTTTGCTTTAAAAGTCAATTTTTACCTTTTCTGTTTATTTATACGTGTGTTGTTGCATAGATAAGTTTACACTTTGTGGCTAGGTATTATGACAGCAAAATTATTTATTTTTTCAGTTAGAGTAGTTTATATATCTGCTGTTTATCTTGGATTTGTAAACATAAATGCGAGGTCAGCACGATAACTTCATAGTCCTTCTGCAAAGAACGACCGATGATCTGCTCAAAAACCGTCTCCTCTCGTCCTTTGTGAGGCAGATAGTAATCCAAGACATAGAGAAGGTTTTTCGCCATTCGCTCAAGGGAAAAATAAAAAAAACTGACCTTAGTGTAAAGGTTTTCTCGCTGCTTTCAAGCGATTTTCTTTCCTTCCAAGAGTGAGAGAGTTTTTCAAAAGGTCGGAAGTCTGATTTTACCTTTTTGGTAGCTTTACGGTGATGGTGAGAGGCAACGTAGTTTTGTTGTAAATGAGGGGAAAGTGCGAAGATTTGCTAGATAACTGAAACAGTGAGAATGAACATGATGAGTCTAGAATGAGGGCTCTTACTTGAAAAGGCAAAGGAGAAAAATATAAGAAGTTGACATTCTATATTTATTTAATATAAGCACACTATGAGAAACAATATAAACATGTATTTAAAAAATTTGATAGACGGTAAATTAAAAGCAATTGAAGAATACTTTTCTTGTGATGCAATATGATATTTTGGAGAAATGACTCCAACAGTATTGAGAAATTTTAGAGACTTTATAGAAGAGCTTAGGTGAAGGCCAATAAGAAAATATCAAAATCCCGATAGATTGATAATTGTATTAAATACTCCATGATGAAGTGCCGAAGCTGTAGAAAAATTAGTAGATATAATTAGACATCATTACAATGAAGTGTATTTTATAGTTCCTGATTTTGCTATGTCTGCCTGAACTATTTTTTGTATGTCTTGAGACAAAATTTATATGGATTATACCTCATCTTTATGACCTATTGATCCACAGATTTATAGTGTCAAAGAAAATAGATTTGTCCCTGCACTATGATATTTAGATAAAATTGAAGAATTAGTTGAAAAAAGCAGAGCTAATCTGATTAGTCCTGCGGAAATGCTTATGATTCAATCCCAAGATTTAGCAATGCTAAAATCATGTGAACAAGCGAGAAATCTATCTATAAAATTATTAAAGGAACGACTTGTGAAATATAAATTTAAGACATGGGAAACTCATTCATGAAACGGAATAGAAGTTAATGAAGATGAAAAAAAGGAAAGAGCAGAAGAGGTTGCTAACATTCTTTCCGACAATAAAACACGAAGTTCTCATTCAAGATACATAAAAATTGACAAATTAGAACAAATAGTTAAACTTAAGATAGATGATTATTCAGATGATGAAAAATTAACGAAAATAATCAGAGAATATCATGATCTTATTACAGAACATATAGGATTTAATTGAAGATGAGTATTCCTCCATTCAAGGTATTTCTTTTAAATTATATATTAAATATAATGATGTATATTGGTTTGAAGCAAGAAATAAAGAAAATAAAAAAGAAAAATAATAAAGATTTTTGCTGTAGAGAAAAAAATAATATAAATAAAACAACTTCAACTTCTCTGCTCAATGGCTTTCCTATAGAAAAAAAAAGTTATGACTTTGCTAATGCAAGAACAATTTCTGAAGCCACGAATATTCAAAATTCTGAAAGATTTATTAAGAATAAGAACAATACTAATTCTTTTTGATATTGCTTTCAGAATTCTTTTTAATTATAATCCATATCTCTTTAACAAAAAAGCAACTTATGAGGATATTATTTATTATTCTCATAAAAATAATTCTCCAATTTCAGACCCATATTTACTTTTTACAAAACTAATAATATATCATTATTTATAAGCTGTTTCCCAATTACCATACCTTCCTAATAATCTTTCTTCGTAGTCAGAAATACAGACGATTCTGACTTTTTTCTTTTTTTAAAGAAGTTCTCATAAAAAATCATTTCTTAAAGCTTCTTCTCATCCGTTTCAAAGAAAATAATTATCCAATAAAATAATATTTCAATCCTGAATTTTGTCTACGAACTCAAGCGGATTTACGATATTCTTTTTTACTGGATAATTCCAATTAGCGAAAAGATGTCTGATTCCTTTATAAATTTTTTCACCATATTCATCATCTAAAATCCAACAAATTTTCATAGAAAGATACAAAATAAACCTCTATTCCAAGACCGAAATACATTCAATATGATGAGTGTGAGGAAACATATCCACCCCTTGCACTTGCTTGAAACTAAAGCTTTCTTCTACCCAACGCTCAATATCCCTCGCCTCGCAAGGTCTTGATCTCCGTTTCTGCATTACGGACGATATCAGCCAAACTGTTATTATAGCTGATCACAAAAGTAGATATCTGCTTTTGTAAAAAGGGACTAGCTTTTTTGCCTTTTTGAAATCGTCTTCAAGCTACTTACAAACTGTTTAAAAAAAATTCTAGCATTCCTCCAAAAATCTCAGCTCTACCTTTCTGAGTCCGAATTTAGGATTATGAAATTTTACGATTCCCATATTATTCCGAATCTCTAAAACATAGCCTGTTCCAAAGAGCTTGTGCCTTACTGTAGCTCCCTCAGTGATGCTTGATTGCTGAGCTCTACTGCTTCCTTGAGTGAGGTCGTAATGTTTGATTAGTTCTGAAGGTAATTCTGAAAGAAATCTGGATTCAGGATTCATTTTTGTTTGTCCCCAAGTCATCCTGCTCTGAGCATGAGAAATAAACAACACATCCTTCGCCCTGGTAATCGCCACATACATTAGTCTTCTTTCTTCTTCCAAAAGTTTCGGCTCAAGCATTGCATTTGCTAGCGGGAAAACCGAATCCTCGGCCCCAACAATAAAGACCACCGGAAACTCCAGTCCTTTACTAGAGTGGACCGTCATGAGTTTTACAGCATCAGCATCCTCATCTTTATTTTCTATGGTATCTACCATCAAGGTCACTTCTTCCAAAAACCGCCTCAAAAGCTCCTCTCCCGAACTTACCACTTCTTGATTCGCATTACTCGCTCAAAATTCATATTTGCTCGCCATATTGATCAGCTGACCAACATTCTCATATTTTTCCTCAGCCGCTTGGTCATTTCCTTCTTCCTTGATCAAATAGTTTTTATAATCAGTCTTTTTGAGGATAAGCTCAATAAAATCCGCTGGATTAAGCGTTTTTAGTGCTGTTGTTAAAGCTTCAAAAGTCTGAATAAAGTTTTTAATCCCCTCAAAAGCTGGGTTCATCAGCTTGATTCAGGTTTCTTGTTCTATGGTTTTAGTATCTGAAAGTTGAGCAAGCATTGCTAAAACCTCATAAAGACTGACTCCGAGGCTGTTTGCATAGTCTTCAAGCCTTTCCTCAGAAGTTTTTCAGATTTTTCTATTAGGAATATTCAGAACTCTCTTCAAAGATACGGTATCAAAAGGGTTATTGATCACTTTCAAATACGCAAGAATATCCTTGATTTCCTTCCTCTCAAAAAACTTGAAAGCTCCAAAAATCTTGTAAGGAATCCCCTCCTGAATAAACATCGTTTCAAAGTTCCCAGATTGAGCATTCGTCCTGTATAAAATCGCAACCTGACCTCGTGACTTCAGTTTTTCTTTGGCTTTCATCTGCTTGATGAGTTCAACGGTATTTGCTGCCTCATCAATATCAGTATTGTGATTGAAAATTGTGATTTTGTCATTCCCTTCTCTGTGGGCAACAATATTTTTCTCATACTGGTTTTTATTCTGCTTGATCACAGCATTTCCAGCATTCACGATATGAGGTTTGGAGCGGTAATTGGTTTGTAGCTTAAACATCTGAATATCAGGTCGGTACTGTTTTACATTGAGAAAGTTTTCCATCAAGGCTCCTCTCCAGCCATAAATACTCTGAAAATCATCTCCAATGAGCGTAATATTCGCTCCCGTTCCGCTCATGAGTTTCATCAGTTCAAACTGAATCCAGTTCGTATCTTGTGCCTCATCCACGAGAATATAATCAAAGGCATTCTGCCACTTTTTCAAAATCTTCTCATCCTGCTTAAAAAGCAAATACGGCAACAAAAGCAGATCATCAAAATCAAGAGAATTTGCTTTCTCAAGCTCTTTTTCATATTCCTGATAGAGTTTTCCCATAGAGGCTTCATAGTTTCCATTGGCATGTTTGAGAAAAAGCTCAGCAGTATTTCCGTTATTTTTTTGTGTAGAAATAAAACCCTTTACCTCCTGTGGTTTGAAGGTGTCGTTTAGAGCAAATCTTTTGAGGAGATCTCTGATAATTTTTGAACTATCATCACTATCCAGAATTCAGAAATTTTTAGTAAATTTTCTCCCCAGAGCTTCAATATCTTCTTTGAGAATTTTGAGAAAAATAGAGTGAAAAGTCCCAATCCATTTGAGTTCTCTCGCTTCTAAACCTTTTTGGATTTTGGCAGAACTTTGCATTTCAGAAAGAAAATCTGAAAGAGCATCAGAGTCAGCTTTTTCTTGTTGTTTTCCTTGATCAAAATTCTGGCTTAAGATCTCATCTCCCAGCTTAATCAATCTTTCCTTCATTTCATTAGCGGCCTTATTTGTGAAGGTTACGGCAAGAATCCTCTGCACAGGAAGCTGCAATCCTCGGATAAGATAAGCGATTTTGTAGGTTAGCACTCTTGTTTTTCAACTTCCTGCTCCTGCGATAATGAGTGAAGAAGTTTGGGTATGAAGTGCTGCTAAAGTCTGTTCTTCATTGAGATTTGATGCGATATAGTCTTTGAGGTTCATGATCGTTTTTAGATTAAGAATTATGTATTGGAGAACTGAGTTTTAATTCAGTATTTTTTTTCTTTTTTCGTGAGATTTTTCTTTGCTTTGTAGTGTAGGGAAATGATTGCTGTTTTCAATTTGAGTTTGGAGAATTTGTTTGATCGGTTGTAGAGTGCTTTTTAGAGTAGAGAGTTTTTGGTTTGCTTGTTAGAATTTACTTTTCTCCTGAGATGATTCGTGCTTTTTACCCTTTGGAATCGGAGTATTTTTCGTTTTTGATAGCCTTCTTTATTACTGGGTTTTTGTTGGATTTCGAACGATACTAAGCTGACTCTTACCATTTGCCTATTCTCAACGAAAAGGTGATTTATTAGTTCTTTATAGCTTTCTTTTGCTGTTGCTCTTGGACGGTTTGCGGACTGAAAATTTTTGTTGATTTATTTTTTTAGAATGTTATAGTATTGTTATTTTATTTTTTTTAATGAGGAAAATGACGAAGGAACAACTTACAGAAACACAAAAGGCAGATCTTATGAAAACTATGACTCCACGTCTTGTGATGTGTATGGTTCTTTGACCTCTCTATTTTATGGTAATGGTGTTTTTGCAAAAGATGGAGACTTCTCTTTGGGTTTCTCTGGGAATTTCTCTTGTGTATGTGATTTTGATTGTGGTGATTACTTTCCCTTTGACTAAACGTAGATAGTTTTGATTCTGATTTTTGAAGCTTTTTATTGCTCTTGATTTTTTCTCTTCTTTCCATATCTTTTGGATGGGTATTTAGTCGAGAGAATTACAATAATGAAAAAGGTCTTTAAGCCAAAGCTTTACAGAATTGTGTTGAATGGATTTTATGGGATTTTTGCTGGAGTCCTTGTTACTGTGATTGTAGGCTATATTTCTAATAATGTGGTTTATGCTTTTATTGGTGGGATTCTTGCTATTATTCTTTATGTTTGGTGGCTCATTATCAATAGGTTTATTACTATTGAAGTAGATTCTACTCATCTGATCGTCAAAAAAGGAAAAGAGGAGCGTTATTTTGAAATTGCTCAATGTGCCTTTCGTGCGAAAATCATCACAAGAAGCTGAGATACAGAATGTTGATTGTATGTTGCAGATACGGAAGGTAAAGAACATTTTATTGATTGTGAACTTATTGGAATTGAACAATTTTGAGAACTTCTGGAGTGTCTTGGAATCTGGGATAATCAGCCTTCTCCTTTACTTCCTTCTCAGAAAAATTAGTTTTTTTATTCTTTAAATTCTATATTCATCATGTCGACTGGAGCGATTCTCATACTTGTTATTTTTATGGTGGTTTTGCCTGCTACTTTTTACTTTTTGACGAAGCAAGTCTCAAATAAAAAGGTGCAAGCCTTTCTTGAACAACATCCTGATGCTGTAAAAGTTTACCTTCCTGGACTTGGAATGAGCGGAGTGCATGCTGTAAGTGTTGCCCTTGAAAGTGTTGATGGTGAAAAGCCTCTGATTTTTATGGAAGGGATGAAAACGGGATTTTATGTTTTGCCAGGTAAGCATACTTTAGAAGCTACTGCGACAAAAACGAGAGCTGGAGTTCTTCACAAGAGTGTTTCTACAGTTTTTGGTCCTTTTAAGCATGAAGTGCAGGTAGAAGCCTACAAGATTTATCAATTTGGTTTTGATGGTAAGGAAGAAGTTTTTACTTTTTCAGAAAAGAACTAAAAAGCTGATCTCTTGCTTAGATTTGGAGAGATTTCTGGAGTGTAGGGTTTTTGTTCCTTTTTTCCTTTTTTCTCTTGCATTTTAGAATAAAATTATTAGACTGCAAATGTATTTAGTTTTCGTTACTGTGATTATGAAGCTTTTTGTTGCTAGCTTTACTCTTAATAATAATCTACGGCTACCGTAGATAGATTGAGTTGTATACGAAAACTTCTAAAATCTCCTACGGTAGTTTATAAGCCGTGGGGGATTTTTTTAATCTGGATTTTATTTCTTATATAGTATTATCATGGAAATACTGTATGCAAAGGCTAGGCGTTATCGCTCATGCTTGGATCTCAGATCCACCCAGCAGGCAATCCAATTTGTCAAGAGAAGCTTTGAAAAGCAGCTGGAACACTTTTTATCTCTGGAAAAGATTGAAGTTCCTCTCTTCGTAGCAGCTGAAAGTGGGCTTAATGATTCTCTTACAGGAACAGAAAAGGCAGTAAGCTTTGAAATCCAAGCTCTTCCTCAGCTAAGAGCAGAAGTTGTGCATTCTTTAGCAAAATGGAAAAGAATGGCACTTGATTTAGGCTGGTTGCGTTTCTTCTCCAAAAAGCACATATTTAAGCAGTTCAAAGCTCAATTTGGTGATCAAGCTTGGAGCAAGAACTTTATCAAGCAGGAATTCAGCTCCTTTAGCTCTTGAAAGTATCGTCAAAAAAATTATATTACTTCACAATATCCTTTTTATTCTTTGTTCTATACATTATGTCTACAACTAGTATAAAAACTCTATATCAAAGCTATGAAAGCTTGTTTGGAAGTCAACTTATTGTAAAGGGGCGAATTAGAAGTCTTAGAGATTCCAAAACCTTTGGATTTATTGAGCTTAATGATGGTTCTTACCTAAAGAATTTGCAAGTTGTTTTTGAATCAGATTTACAAAACTTTGATGAAATCTGTAAATTTACGACGGGAAGCAGTATCGAAGTGCAAGGAGAACTTGTAAAATCTGAGTGAGCGAAACAGGCTTTTGAACTTAAGGCGAGCAAAGTTATTCTTATTGGAGCGGCAGATGAGACCTTTCCGCTTCAGAAAAAAAGGCATTCTTTTGAGTACCTTAGAACCATTGCTCATTTGAGACCAAGAACTAATACTTTTTCTGCGGTGTTTAGAATAAGGAGTCTCCTTGCCTTTGCTATTCATGATTTCTTCAATAAAAATGGATTTATCTGGGCTCATACACCACTCATTACCGCAAGTGATTGTGAAGGCGCAGGAGAAATGTTTCAAGTGACCACTCTTGATCTCACAAATCCTCCAAAAAAAGAAGACTGATCCGTTGATTTCAGTAAGGATTTCTTTGGGAAACAGGCTTCTCTTACTGTAAGTGGTCAGCTCGAGGGAGAAACGTTTGCGACTGCTTTTGGAAAGATTTATACCTTTGGACCAACTTTCCGTGCTGAAAACTCAAATACCACAAGGCATCTTTCAGAATTTTGGATGATTGAACCTGAAATTGCTTTCGCTGATCTTTCAGACAATATGCAACTTGCCGAAGATTTGCTCAAGTATGCTTTCCGTTATGTGCTAGAAAATGCTGAAGTAGAGATGGATTTCTTTGATAAATTTATCATGCCATGAGTAAAAGCCAGACTTGAAACTCTTGTAAATGCTTCTTTTGGAAGGGTAACCTATACTGAAGCGATAGAACTATTGAAAAAATCTGGGCAAAAATTTGATTACCAGCCAGAATGGGGAATTGACTTGCAAACTGAACATGAGAGGTATTTATCAGAAAAAGTCTTTAATGGTCCGGTTTTTGTTACTGATTATCCAAAAGATATTAAGGCTTTCTATATGAAGCTCAATGATGATGGGAAAACGGTGAGAGCTATGGATTTGCTCGTTCCTGGGGTAGGGGAGCTGATTGGAGGAAGCCAAAGAGAAGATGATTTTGAGAAACTTAAGAAAAGAATGCAGGAGATGCATCTTGACACCGAAACTTATAGCTGGTTTCTTGATCTCAGAAAATATGGAACGGTGCCTCATGCAGGATTTGGAGTGGGATTTGAGAGGCTTGTAATGTATGTGAGTGGAATGGAGAATATCCGTGATGTGATCCCTTTCCCAAGAGCTCCAAAGACCTGTGAGTTTTAATGCTAGCAGGTAGAAAATGAATAAAGGGAAAATCAATCGCAATATAGCAGAATCTTTTAAAAGGCAGAGACAAAGAGAGATTATAGCTTTGAAAAAGGAAAGAGATGGTAAGAGGAGAAATTGAGACCTAATAACCGCTCTTGACTATCATCCTACGGGGCATATTAGGCATGGGAAATAAGAGAAAACAAGAGAAAGAAGTCAGATTTTGATTTCTTTTTCTTTATGAGTAAGAAAGTATGCTATGCAAAGAATACTCTACAACTACTTTTTCTCTTTTTCTTGTATTAAGAAATAGGATAGCTATAGTGAAAAGGGATTTTATCTTTTCTTAATGATGTCAAACTGTATCTTCTGCGACATTATCGCTAAAAAATTACCAGCTTATCTAGTAGCTGAAAATGAATATGCAATTGCCTTTCTTCCCAAAAAAATGGAAAGTTTCGCACACACTTTGATCGTTCCAAAAAAGCACTACGAAAATCTTTTTGATATTCCAGCAAACGAATTAAAAAATCTGATTTCCTTTTTGCAAAATACTGTGAAATCCTATAAAATTTGACTTTGAGCAACAGGTATCAATATCTTAAATGCGAATGGAAAAGATGCTCAGCAATCTGTTCCTCATCTGCATTTTCATCTTATTCCTCGTTTTCCGGATGATCATTTGGATTTACGACCAGATTTTCCTAATTATGATTGAAAATTTGAGGAAGTGCTTGCAAAAATAAAAAGGAAATGTCTCTTTATTGAGAAATAAAATATTATGACTATAATTTGATTATCTATTCAAGATCCACTTTGGAAAAAGGTTATTTGATATGCTAATGATTGTCCATGGGAAGCTGGGACAATTTTAGCACAGAAAATGATTGAGAATGATTTTTCCGATTTGGAAAGAGTGTTTGTCGCTATAGAAGATGATAAAATAGTTTGATTTTGCACCTTTACCAAAGAAGACTGAATTCCGAATTGTGAATATGTTCCTTTTGTCGGATTTATCTTTGTAGATGAGGGGTATCGCGGGCAAAGATTGAGTGAAAGACTGATCAATTCTGTACAGGAGTATGCAAAAGCTTTAAATTTTAAAAATCTGTATATTGTCTCGGATCATAGGTGATTGTATGAAAAATATGGATTCAACAAAATAGATGAAAGTATTGATATCAAGGGTCGTAGAGAAACTATTTTCTGTAGAGCAATAAAAGAAAAAACTGATCAAACCGTCTTTTTGACGAGTAAAATTTGCGAAAAGCAAGAGCTAGATTGAAAACTTGCTCTATCAAAACTTTCAAATGAAAATGGTTTTTTGGATAATTTGAAAAAGCAAATAAAAACACAGAATACATTTGTTATGGTTGCTAGTGATCCTACTGCTTATGAAAAAAATGATCAATTTTTACAGCTTGATAGGCAAGCCCTGACTCTTTCAGACTTACATTTTCAGAAATATCTTGTCTTGGATAATCGTAATAAAGATAAGGTGAAAACTGTTTTGGATCAGGCTTCTTTAGTTATCCTTGCCTGAGGCAATACCTACGAACAAAATCAGTTTTTTAGTACAATAGATTTGGGAAAGCATTTGAAATCTATTGACTGTCCGATTGTAGGAATTAGTGCAGGAGCTATGAATTGTGGAGAAATCGTAATCAATAGCTCAGAAAAAAGTAAAAATCCTGATTTGCCTTTGATTTTGAAGGGAATGGGGATTTCACAATATACTATAGTTCCACATTTTGAGAAAAAGCAAAAAAATCCTGATGAAATGAAACTTATCATTCAAGCCTCCCAAAAGATGAAAATTTATGCAGTTCAAGATGGTAGTTATCTCTTGAATGATACAATCTATGGAAGATGTGATCTCATATATCAAGGGAAAATTACCAAGATTTGTGATATTTGAGAAAGTTTTTTATTAAAAAAATAATCAGATTTTTTTCTTCTTTTTTATAATCTTTTCTGCAAAAATTGCTCAAAATGGTCTGTAGGGATCAGTTCTTTCTAAAGAATCTGCTAGAACAGCCGATAAAAACTGACTTTTTGCGAAGGATTTGGCTAAAATAAGCGATTTTTTTGTTTTTTGACTGAAAGAGGAGGCTAAACTTTCCGTTTTTCTTGATTCAGAATTGTTTTTTTCTCTTGAAAATTGGAATGATAGCTGTATATAGGAGCTAGTTTTATTTTCTAATCACTATTATTGATGAACCACTTGAAATTTTTGGTGCCGCTAGTGGGAGCTGTGCTTTTGGCGGGATGTGCAGGTGATCAACCTTCTCAAGTAGCTATTGATCAAAATCCAGCTTCCAAAACTGCGTCTTCTGAGAGTCTCTTCTGAAGTCGTAAGTCAGATTTTTCTTATGCGACGAAGATGCAAAAGCCTGCTTTTGATGCTCTGAGCAACAAGGATGAAGTTCAACAAGAAACTTTTAATAAGCCTTGGACAAGGCAGTATCAGTTGACTCAGCAGGATATTGATCAGTTTAAAGAGAATATAAAAGGAGGGTATTGTAAAGATAGCTTTGGGCTTTCTCCTGAGTCTTGAGATGGTGATGTAGAGCTTCGTCCTTCTGATTTGCAGGTAGGGAAAACATTTCCTGTGACAGGGAAGTATTCTGTGCATAATTATTTCCCTTTGACATGAGCTCTTTTGGCTCAGGTAAAGAAGCATGGTTTCTGAATCTCTGAAATTACAAGAGAATATCTTAATCCAGAGTATAAGGAGGGGTATGATGACTTTTTTAGTAAGGAAGAACGAGTCAGAAGGATTCAAGAAATTGGTGGAAGTCAGGAAGCCTATGAAAGAGCTCCGTTTAATACGCTTCTTGTTACTGATGATCTGCTTCTTCACACTTTTCACAAGATTTTTTCTAATGAATTGCAGTATTTTGAGGAAAGTCAAGCGAGACCTGTATTGGCAAAGCTTAGTGAAACACTTTTCAAAAAGTTTCAACTCTGATCCAATACTATGGATCCTTTCTTAGCAGCGTATTGGACGATTCCTCATGCTTTGCTTCCAAGTAATGAAGAGTTACTTGAGCTCTTTAGGAAGAGAGAAAGTACTATGCGAGATAATTATGATCATACTTCTGGAAAAGATCCTCATGCAGATTTTGATGATGAGGAACTGAAAGCCTATCTTACTAAGAGATTTGAAAAATTAGTTAAGCAGGTAAAGCCGGAGTATCAAGAACCGCTTAGGAAGGTTTGGACAGAGATTTGGAAGGCAGAACAACCAAAAGCCCCAGATTATCTTATGCTAGCATATAGTCCTGAATTTATTCAAGCAAATGCAATTGAGCAGGACTATACCCAGTTTAGACCTAGGTCTCATTATACTAATTCTTCCTTTCTTAAGTCGTATTTTATGGCGATGAAATGGTTGATGAGAGAAAAGTTTTATTTCTGAGATCAGAAGTTAGCAGAAACGGCTCTTCATCTTGTCAATAGTATTCCTGATACCGATCTCAAAGAATTAAGTCAGCTCCAAAATGCAATTCTAACCTTGATTTGAAAGGATGACGATGCTACGATTCAGGATTTAAAATCTTTTATCACTCAGCAAAACGGCAAGATCACTGGACTCTCAGCAGAACAACTTCAACAGCTTGCTCAGCTTGGAACTCAAAAGATTATCTCTACTTCCTATACAACTCCAGCAGTTTGAAGTACTGATGAGGCAAATGCAAAAGATATGACAAGAGGATTTGTGTTTTTCGGAGAGAAGTTTACCTTGGATAGCTATATTTTTGATCAGCTAACAGCAGGAAGTGCTGAAAAAGAGTTTTTGCAGAAGCCTAACGTCCAGACGGCAATGATTGTGCCTGATGTATTAGAAAATTATGCTCCTGCCCACGAGCTTGTAAAGCTTCGATTACAAGAAAAATCTCGTGGAGATGATGCTCAGGTGCGTGAAAATGTGAGCTGTGATTTAGGAACTTGTAAACAACTCGGTTCATATGATCAAGTAAAAGCACAGGCTCAAGAAAAAGTGAAAGCAGAACTCAAAGACGAATCGCTCTTGAAAACGGTGTATCATAGTTGGTTAGAGATGCTCGGACAGCTCTTTGTAAAGGTTAAGAATCTTCCTTATTTTAAAGAAAGTCCACTCTACGCCCTCAAATCACTTGCGACTTATATGGGTTCTTATACTGAACTTAAACATGATACCTTGCTTTATGTTAAGCAGGCCTATGCTGAGCTGGGAGGCGGTGGCGATGATCCTTGTTATATTACCGTGTATCCTCCTGAGTTGCCTGTGCCAAAAGGGTATATTGAGTCTGATGTGAATTTCATTGATAAAATGATTACCCTTAATCAGTCAGCACAGCAGTGGTTCAACGATGCAGATAATTTCAAAGGATTTGGTGAATATCTTCAAAAGATTAGAAATCTGAGCCTCAGACAAATGCAGAATCAGAAGATTTCAGATGAGGAATTTGAAGATTTAAGATTATCTTATGATGAATTAGCAAAACTTACGACTCCACGCAAACTTTTCTGAGAAGCTCCTTCCAAAGAAGAGAGAGGTGCTCTGATTGCAGATATCTTTACCTCTGAAGGTGGAAATCCGCTCTATGAAGCTATTGGTAGACCAATGTTAATGGCTTTGATGGTCAATGATGTAAATGGAGCGAGAATTGTGATGGGACCAGTTTTTGCTCATTATGAGTTTTATAAGTCTGATAATGTCCTAGAGGGAGAACAGAGATATTCTGATAATCAACGACAAGAAGCCTATGACGGTCTTACTTCAGAAATGAAACAGAAATATTACGGAATTGAACAGAGAAAACTCTTACAGGAACTAGCAAAATAGTCTTGATATTCAGTATTCTAATAACTATTTACTTGATATTCTTTTTATTCTCTTAGCATTTATGATGATGAAAAAGGTATTGCCCGTTTTAGTGCTTTGTTTACTTGCAGTAGGGTGCACGAGTTCTACCGAGCTTGGAGCTCATTTTACGCAGTCTAAAGCGTCTGTAAACTCTCCTTCTGAAGGTTATACCGTGAGGGAGGGGGTTATTCGTCGTATTGATCTGATCGACTGGGGAAAGTTTGGTGAAACCGAAGAAGATGATCAGCAGTATACTGCCTGTAATAATGATAAATCTTTGCGAGGGACGGAGAAATGTCCTATCTTTTCATTCCTTACTTTTGTTCCAACTGATGGGAAACTCATAGAAGATGAATGGTATCCTTCAGGTGTTGAGCTTTTTGAAGATGATAGTAAAAAGTCACTTGATCTTGGATGTTTTTCACCAGAAGAGGGAAAAGGCGATAATTATGTCCCAGCATTTATCTATACCAGCAGTTTTGATGAGAAACAGAGTGAAAATGATGATTACACCATCATGGATAAGGCAACAGAAGATGCTCTAATTAAGTTGGTTGATACCGACAAAAAGGTAAAGCTCAAGTTTACTAAGACTCCTTATAATGTTGCTACAGATTTTAAATGGGCAGGATGTGAATCTTTGATAAACAAGGTAGAAATTGTAAAATAGTTGGCGGTATGCTGTGATGGATACTAGATTAAAAATCAGAATGGATCTCTTGATGCTCTTGATCTTCGAATTGGGAGCATTTTTTTTGATATTTTTCTTGGAATGTCAATATTCTTTTTTTGATTTTGTGGGATTTTTTTCCCTTTTTTGGGAGAATTGACTATACTTAGTGTGAGGATTTTATATGTTAGTGTGCTTATGGCTCGTAAAATAGAACAAATACAGCAAGAAAATATTGCTATTCAGAAGGAATTAGATGCTCTCAAGGCATTGACTGAGCGTGAAGAAAAAGAAAAGAAAAAATCAGAACTTGATCTTCGTATTCAGCATGCAAAGAAAGAAGTTTCGTCTCAGTATCAAGCAGAGACCGACCAAAATATGCGTGAAAAGATAAAAGCAGAGGAAAATAAGCTTGAGAGTTTTTCTACTGAATTAGGAGCATTGGGGGTAGAAGTTCAGGGAGAACAGCCTGCTCATCCGGAAAAAGAAGTTTCGAAAGCAGAAAAAGATCAGAATTCTTCTACTGAAAATCCATGAACCTGGAAGCAAGTGAAAGATTTTGTCTCAGAGCAATGGACTGATGTTTGGGACGCAGATAAATGGAAAACGGAGACGGGGAAAAATATCCTTCGTGCGGCTGGATTCGTTGCTACGGGAGTCTGAGCGGTGGCGTTGGTTTATAAAGGGCTAAAGTCTTTATTTTGAGATGAAAAAAAAGAGAAAAAGTCCAAGAAAAAGAGAACTACATCTGAGGAAACGAGTTCTTCGGATGAAGATTCTGAAACTGAAAAAAAAGATAAGCCTTGGCGAAAGAAGGCATTAGCTCGAACATTAGGAATTTGAGGAGCTGCTGCTGGAGGACTTTTGGTCTATAAGAATTGGGATACGATTAAGTGATGGTTCCTTGATGTTACAGGAAAAAATTTGAATAAAGAAGAGGCCCTTGCGAAAGTGACTGCTGAGGTGAAAAATGGGATTATTGCTGAGGATAAGTTTTGAGTTTTTTCGGCACATTTTGATGGAATTACGTATGATGAGACCACTTGAATGGTTTGTTCGTATGGAGAGCAGATTCCAATTGATATTGGGGCAAGAAAGATTGTAGGGCTGGATGTGCAGTTTTCAAAGCGAGAGGAACTTTTTCATGCTGCTAATATCGTGAATTTCTGTAGGAGGAAGCTTAGATGAAGGGGCGCTAATGCTCAGCCTTTTGTAAAAACTGAGAGCGGAGGAGATATTTGTTTTAATAGTTCAGCGGAATGAAAGCAGATGGCTGTGTCTGCCAGTGGTTCTGATGAGTGGAAACGGATTCTTTGAACAGTTGGAACCGTCGGTGGCGGACTTTTGGGGGGGTATCTTGCTGGTGCAACTGGTGCACTTGCTTGAGCTGCTGCAGGAGGAGCGACAGGAACGGTAGGAGGTGCTGCTCTTGATGATAAATCGAGTGTTAATAGCACGTGTTCTATTTTGGCGAAGGAGCCTAATCTTTCCAGGTTTATCAGTTTGCTTAATCAGCAGAAATATTCTGATGGAACTTCTCTGTGGGCATGAGGGAAGGAATATTATGAGCCAGATAATACCTCTCCTATTCATCCATATTTAAATAAAGTGAAGAAAAAGATTGATGAGGCATATTGAAAAGAGGATAGTGTTCGTAGGGATCTATCGGCTGAGGTTGATCCTAGCAATCCAAGTGTTTTTATTATCAAGTCTTATGGTCAATCGGTAAAATTGTCTTTTGAAGGAGATGATGTGCTTTCTGGAGGAGTGATCAATTTTGATAAGATTCAGAAAATCTCTCTCTGAAAGTATGATGAACAGAATGATTGGGGATCTGGTTTGAATCTTGATTTTCCTCATAATGAAAAGGGGCTAGAAGAGGCGATTATGGTTGCGAATCTCACCAATAAAATTAGAGAAGAATTTAGTAATAAGGCTGCTGAAATGATGCCGTTCTCGTATGGAACTTACAAATTTCCTTTTTCTTTGGATATTGATACTCCGGGCTTTGCTGGGAAACAGGTGCTTTCACATGATGCACTTAAAAAATCATTCCCTACACTTCTCAAAGATTTATCTCAGCAAGGGAAGCTCTCTTCTCAGGAAACGCTTCGAAATCAAGCTAAAGAAGACAAGAAAAAAGGTTGTTCTCAATACATTAAATATCTTCACCAAATGCAGGGAGAGTGACAGACATCCTATTGGAAGGTGAAATAGTATTTATATTCTTATGATTTTGCTATGGCAAATCTCTTGGAATCGATCAAGGATCTTTTAAGCTCTGAAAAGGAAGCAGATACGACGTCCAAAGAGCTTATTCAACTCAAAAAAGAAGTAGCTCAAAAAGTTGTCGATGATGTAGAAAAGCGTCATCCTGATTTATTTGTTTCTGCTTCAAAAGACCTTATTGCAGATTATCTGGTTTCCGATGGTTTTTGGGATTCTGTGATGGATAATCTGGTTTTGGAGAATACCGTTTTGAAAATGACGAATCAAGCCCAGAAGTTTCAGCAGGTTAAAGAACAGATTGATCAGGCTAAGACCAGAGAGGAGCTTAAACAGCTAGAGGATTCTATCCTCAATGGGAAAACAGCTTTAAATTCTGATCTTTCTTCTGTTCAGACTTCGTCTGATGTGTCTTCTGACGTTGCTGCTCCTTCTGATGTTGCTGTTCCTTTACAAGAGGATGTTCCTCCTGTTTCAGCAGTTTCGTCTTCTGCTGTTGTTGCTCCTACTCCTATTGTAAAACCTCCTCAGTCTTCAAGGCAGGGGAAAAAACGTTCTCAAGGAAAAAAGAGAGAAACATCGAGAAGTCAGTCTTCAGTGGATGCTTCTCAATATCATGAAAAAATTCGTGGATTTGATGCTAGTAAAATAGTTTCTTTACCATTTGCTAGGAGTAAAAATTGAGTAACACTATGTGCCTCAACTGCGAGGCTTAATGCACAGAAATTCGGTCTGGTCTTACCTTCTGGAGATGCATGGAAGGCTTCTACTAAAAATCCTGTAGCAAAGGAGTATAGATTTTCTCTTCCAAGTGCTAGAAAGCATGAGAAACCTAGAAGAGGGCGAGCCCCGCTTTCTTTATCAGATTTTGATGCGATGCCTGGTGTAAATATGGCGGATATTTATACTGCTTCTAGCTCTAGGTATTGACATAGGTCATTAGCATTTAGAGATATTTCTGGAGAATGGATGGTGCTTGATCCTTATATTAGAGTGCCCTGAACTACTCAAACTCAACCAAAAAAACTCAAAGACTATATGAAGTCTGTTAAGGTCATCAAGAGTCATTTTTATGCGGTAGAAGCTTAAGTTTCTTTATGAACAAAGTTTCATGGGAAAAAAGAGAAGAAGAGTCAGCTTTTATTTTTATTGCTGTTTCTTTTTAATTCCTCTTGAATATCACAATCTAATCTATATACTCGGCGACAGATGTTTTTTTATCTTGTTTGAAAAGCTTATGGCACATAAAAAAGCCGCTGGTTCGGTAAAAAATCTTAAAGATTCTCAGCCAAAATATCGTGGAGTCAAAGTCTTTGGTGGACAACCTGTTCGTGCGGGAAATATTATTATTAGGCAGAAAGGAAGTAAGTATGAATGCGGTAAAAATACTTATACAGCAGCTGATTTTTCTGTTCATGCAGAGGTTGATGGAGTTGTTTCTTTTAGAAAAAAGAATTTTAAGAAGTTTAACGGAAGGACGTATCTTAAGACACTTGTAGAAGTGGTTGATTCAAAATGTGCTTGTGCAGCTTCTTCATCTGCAGCAAACGGTTCAGAAAAGAAGATGAAAGCTCCTTCAGAGAAGAAGGTTGCAAAAACAGCTGTAAAGAAGCCTGCTGTGAAAAAAGCTCCTTCTAAAAAAGCAGAAAAGGCTGAATAAGTCAGCTTTTTGAATTTAGTTTACTAACGGTATCAGACAAATGACAATTGGTCCAAAGAAAAAGATCAGTAAAACCCAATCACATAAAAGACATTCAACATGGAAAACAATTAATCTTAAGAGATTGTCTAAGAAATATCAGACAGCAAAATGTCCTAATTGTGGAGCAAATAGGCTTAATCATAGGGTTTGCCCATCTTGCGGTTATTATAATGGAAAGCAGGTGCTTACTATCAAGGCTGCTAAAAAGGATTCAGCGGTTGTAGAAGCTTAGTTTCTTAGGTTTCCTTGTCTCTCAGCGGATAGGAGGGATATTTTGATTTATTTGAGGAAGCTGTATTCGATGGATATTAGGAAGAGAATCAATGCTAGAAAAATCGTGCTTTCGTATTTTTATCAGCGTTGTTTTTTTTCTGCTTTAAGCAAAAAACCTGTTGTTGAAGAGTTTCCTAGAGAAAATCGGGCTTTAGAGGACTCTGTTGAGGAAAGAAAGGAGGAAGCTTTTTTGAGTACGGATTTTTTGAATGCTGTTGAACAAAAAAAGCAGGAAGCTGAGCAGTGGTGAGAGGTTGTCCAAGCACAAATTAACGCGTATGCTCTTTGAGAGGCGATTGATGAGGATTTAGCATATATTGTTCGTTATTTTTTTGATCAATGGGGGAGTGAGGAAATAGATATGGAATATGTTTTGAGGATGGGGAACGCTTTTTCTCTCTATGAAAGTGTGCTTAAAGAGAAAGTTGATCGTCATGTTCAGAGTTTTTGATATGAGAGGATGGATATAATGGATCAAGTTTTGTTTCTGGTCTGATATATTGAGTATCGTCTCTTTGCTACGCCAAAAGAGGTGATTTTGAATGAAATGGTTGAGCTTGCAAAAAGGTACTCTGATGAGGGAGCACCGAAACTCCTTAATGGTGTGCTTCATGCTATTTTTCAGGAAGAATAGGTATAATGTATTTTTTAAAAATGAGGTTTTGAATGCCTCATTTTTTTTATTTTTGTTGAGGGGATAAAGGTGTTCAATCTCTGTTGGAGAGGTTTTTTAAGTGATTGGCAAGATTAGCAAAAATCTGATCAGGTTTATTTCTCTTTTTTATATCTCCAAGGACTCGATCTAAAGCTACTTCTAATAATTCTCCGATAATTTTTCCCGGTTTAAGATGGAAATGCTCCATAATATCTTTCCCTTTTATTGCAAGGTCTTTGGCTCTAAATTGTCCTTCTTCTTGTTCTAGCTCTAGAAGAAGTTTTTTGAGATATCGAGAATCACTGAGGTCACTTGCATTCTGAAGAGGGTTATACATTCCCATTCTATCTGCGATATTGATGTCAAGGAGGTTGTTTACTCTTTCAAACCCGGCTTCAGAGAGGAGTTTTCTCAGTTTTTTGGCTCGATTCTGAGGATTTCAAAGGAGAATTTCTCAAGGAGTGTGGTGCTCTCTGATATATCGCTGAATCTCTTCAATCTCCTTGTGAGAAAATCAAAGTTTTCTAAAATCCTCTTCAGCAAGCACTGCTGAACTGAATCTATGGTTGAGTGGACCTGCGAGGAGAGTTCTGATTTCTTCTTTTGAAAGATTTTTCCCATATTCCGCATATTGACCGACTTTCCCGACATCATGATAAAGCATAGCAAACCTGACTAAGTAATTTGGATTAATTGACTGAAGATGATAAAGTGTCAAGATAATATGCGAATAAATATCAAAAGGATGGTATCTGATCGGCTGATCAACGTGTTTGGTCAGTGCAAGAGCAGGGAAAAGAATTGGTAACATTTCTGCCTCATCCAAAAGTCCAATAAGACCGAAAGGATTCCCTTGAGAAAAGGCTTTTGTGAGTTCTTCTTTGATTCTTTCTTTAGCGATAGTTTTGAGGAGAGTATGATTGGATTTGATTGATTTCCAAGTCTCTGGTTCAAAATCAAAAAGTCTGACTTTATTGGTAGGATCAGATTTTTTCTTTTGGGTGGTAGTGAGGAGCTTTTGATTCAAGACATTAACAAATCTCAGTCCTCTCAAGAGTCTGAGCGCATCTTCTTGAAATCTTGTATCAGGATTCCCAACGGTTCTTAGTTTCTTTAGCACGAGATCTTGGATTCCCAACGCTGGATCAATGAGAATGTGAAGTTGAATCTGCTTTTTTTCTTTTTTTTCTCCTGTTCAAAAGATGTAGGCATAAGGCTGAATATCTAGCAAATAATATAAAAAATCAGTATCAAGCTTTGCATTTGGAATAAGCTGAGAAAGAAGTTTCTGATTCTGAACGATAAGAGTTTCACTATTCTGAAGGTAGATAAAGCCCTCCTTATTCAGGATTTTGAGAAACACTTCCTCGCTGATTGTCTTTTCTTGCTCATATATTGGTGTTTTCAGCTTCTGCTGAGTATAATAGATTGCATTGATGGTAAAATCCCTTCTCTGAACATCTAAAAGTAGATCATCACTCCAAAAAATCTCCCCTGGATGTCTACAATCTTGGTAGCCTGTCTCAGCACGAAGAGGGGTAAGCTCATACTTGAGTTCATTATTTTTATCTTGGGGAATTAGGGTAATGGTACCATATTTCTCGGTTATAAAGTGAGAAATTCCATCTGTATTGAGATTTTGATAGATCTCCAAAGGATTCCCTGCCATTGTAAAATCAATATCAAGAGGATCTTTCGTTGTCCCGAGAAGGATTTCCCTCACGCAACCTCCCACCAGGAAAAAATCTGATTTCCCTATTTTTTTTGTTAGAAAGGTATGGTGAGGAATGAGTTTTTTAAGATTATGAAAGTGAAGCATCTACGACGTGATGTGATAAATGACGGCTTTATTTCTAGTTTTTCTCTGATGATATTCAAGAGATAAAGGACTCTTTGCATTTTGCGGTTTTACTCTTGTCTTTAATAGTTTTTTTGTTACTATTAGGATTTGCGGTTTTAGTCTGTATTTTTTGCTATGTTTGTGATTCTTTCGATATCAGATTCAGATAAGCATTTTCTCGTGCCGATTCAAGAATATGAAAAAAGACTAGGAAAGAGCCTTGTAATTGATAATCTTAAGCCTTTTAAGGATGATAATGTGCAGCTTGTTATTGAGAAGGAAACAGAAAGTCTCTCAAAAACTTTACACAAAAAATATCTTAATTATCACAAAATTCTTCTTATTAAAGAATGATGAACATATGATACTTTGGAGTTTTCTGAGCTGATTCAGGGAGAAAATGTTGTTTTTATTATAGGTTGACCCTATGGTGTAGATGAAAAAAGGTTAAAATCAGATTTTTCTGATCTTACCATGCTTGCTCTCGGAAAAATGACTTTCCCTCATGGTTTAGCTAAGTTGCTCTTGATTGAGCAGATTTATAGGGCAACGACTCTTTGGTCTGGAAAAAAATATCATTATTAAGTATTGACATTTTTTATTTAAATGATTATACTTAGTCAGATTTTTTTAGTCTTTGATTGTCCTGAATAATGTCTGGTCAAACGCCATCTCTGCTCCAAGAATCTCTCCATACCGAACAGGTAAAAAGAAAATATAGTAAGCTCGAAAGAGAAATAAAAAGAAAACTTACTGAGTGTTTTCTCTGGGATCATTTCTCAGAAAAAGCTTTTTTGTGGAAAGATATTTCTTCTGCGGGAGAATTTAAAGAAGATTCTGCTTGTAGAGGACTATCTTTTACTAAAGATTTTGATATTAGAGTTCTTGGAAAAGCTGAAAACAGCAAACATATTATGAATCTTGCAAATATCAAGGAAGATACGACTCGAAAGACTAGAAAAATTTTTGAAGCAGAGATTATTGATCTTATTGAAAGAAGTGTAGATGCCGAGGATGAAGCAATAAAAACTAGATATATTAATGGAGTCAAAAACTTTATAGCAAATAATCAGCAAAGAACGATCAATTATCTAAGAGATAATAAGACCTACCTTGCCTTCATTTCTCAATATCATTTTTTGCAAAAAAGTGAGAAAAAAGAAGAGAGAATTAAATTTAATGTCATATCTCAGGTGGGGAAACTGATGAATGAAAAGTTTTGAGAAAAAGCTTTTGTAAAATCTGTTACAGAAAATGGAAAAGAAACTATAGAATTTTCTGATCTTTGTAGAGATTATCTCTATGTGCTTTTTTACAATGCTAATGCTGTTCGAAAGGACTGGGGAAGGAATCTATGACAAGTTTTTCATGATAGTGATTATTCTTTCATAACAACCAATGAGAAAAAAGATATACGAAAATATTTAAAGGCCCTTCAATATCTTATTTCTAGAGATAAACATAATCCTTATGGGAAAAGAGACAAGGAATGAATTAAGGAATGGGCAAAAAAAACATCACAATATGCTGATGTTTTGCGGGCTCTAACGAATAATCAACTTCAACTAGAAAATATAAATTATTTTTCTCCGCAAGAAGAATTTTGAAAAAAGAAAGACCCTTGATTTGCATGGGATTATTCTACTCAACAAATTGCAAGAATTGGTGGTTGAATTTTTAATAATACTATAAAAACTCATAATAAATTTGAAAGAAAAGGGCTCCCTCCTTTTGAGTTTAGTCAAAGGAATAAAAGCCTAGCTTCTTGCGTAGAAAAAGTTATTGAAGGGAAAAAATTAAATGATATAATTGGATTTCGTATTTCGATGAAAGGTGTAAATACAGATCATTTTGAGGAGATAAAACAAATTGGGAAACAATGGATTAAGGAATTTGCTACAAGTTTAGAGGCTTATCCAGAAAAATATTTAAAAGATTGAAATCTGAAAATCAAAAATATCGTTATAGATAATAAAGGTGTTCTCTCGAAAGATCAGGTTCAGAATTTTATTTCTGAATTAAAATCCCTTGAAGTAGGAGAAATACAGGAAAGAGAAAAACCGGAACCAAGTTTTATTGATGTCTCAGAACGAGAACAAAGAATGAAAACTTATTATAAAGAAGATTGGAAGGATACTGAAAAATGGAAAACTTACAAAACTTTTTATACCCAATTCTCTGGGAATACCAAAAGAGGTTCTAATGGTAACTATAAAGATTTTAAGTTTAATATCACGGTTTGAATTTTTGATAAAGATGGAAAAGAAATCTGAGAAAAACCCATAGAGATACAATTTGATGATATTAATAATGCTGAAGGTCTAGCAAACTTTCATATTAGAAACATTGAGAGAGAGGTTAATACTCAAAGCAATCTGAGTTTTAGTATTTCTCTTTCAGAGCTTAGAAGAATAGCTGAAAAGAGTCTCAAAAAAATGTCGGATCGATCTGAAGATAAGGACGAAAAATTTTCAAAAATTGATTTTGGAGAGGGATTAATAATAGATATTTCAGATTTCTATGAAAGAACTCCTGAAAACGATGATCGTATTAATAAAGCTATTGTTGGAATTATTAACTATTTTCTCAAAAAGGGGACTTTTTTCCTCTACTATCAGGGTAATGAAGAAGCTAACAAACAAGACAATCCTGAAAAAAAGATTCACCTTACTAAGGGATTATTAACTACCAATGATCTCAGTCTTGATCCTTCTAATTTTATTAGAGTTTGTTCTGGACTGGAAGTTTGAAATCAACAGTATTCTTATCTTGGAAGTAAGGCAAATGCTTTTGTCTGAATCTACGATGCTCAGGCTGAGCAAATTCGTTGGGCAAAAATTGGAGAACTTATTGATCGTATGAATCTTGGAAAGAAAAAGGATCCTCAATATATAGCTTAAATTTCATTCATAATTTCGGTCACAATTCCATTAAATACATCCATTCCTGCGTCTGTTAGTTTGAGTTTTTCTCAGTCAATGGTGATTAATCCTTCGTTTTCGTAAATTTGTAGTTTTTTTTCGTAGTGAGGGATTAGGAGAGAGGTGAACTTTGAAAGCTGAGGTACTCCTTTATCTGTTCTTAATCCTAGAAAAAACTCTTCTATTAAATAATCTTTTTTACTAAGTGGCTCAAATGCTGAGGGATCGAGAAATTGCTTGTTGCAGTAGTCCTCAAGATTATTAGTGTTTTTGAATCTCAGACCTGCCTCTGGTGTAGAGAATCCTAGAGAAGAAAGAAATTCTGAATTAAACTCTTTTTTATTTAAAAATGAAGAAGCATTGAGTCCCAAACCGAGATAATTCTCCATTTCTCGATAAGTTCTATTGTGGATACTAGATTTTCCTGCAAGAGCAAAATTTGATAGCTCATATCTATGGTATCAGGCATCTAAAAGAATATCCTTTAAAAGGCTAAACTCCTCATAGATTTGTTCATCAGTTCCAAAGTATGCTCCTGAGATGAGTGCGTCGGATTTTTTTCTTTTTCGAGCTTGATTTTCAAAAAGTTCCAAAGTATAAAGCGAGAAACTATCGGCAAATTGTGAATTTACAAAATCTGTAAAAAATTGTAAAGCTGAAGGAGTCCGCAGGTAGGGATTGCCTTTTTTACTCTGGTTCCATTTTCCAAAAGCGATAAAATCAAAATTAAAGACTGTATTATCTTGTTTGAGAGGCTGGAGGTTGCGGAGAAAATCAACCAGTCCGAGGAAACGTGTGTGTCTTCCTGCGAGTTGAAGCACTTCATTATCAAAACTTTGGATTCCAAATGAGAATCTGATTCTTGGATATTTTTTTCAAAAGGTTTTTTGGAGAGCTTTTATGATTGCGTAAATTTCCTCTTCAGGATAAGGATTAAACTCAAAGCTTAGTTCTGCAAGGTTAGAACAATCAAAGTATTGCTCTACGGTGAAGATAATCTCAGAAAGCTGATCTGCTCCAAGGAGATTTGGAGTTCCCCCTCAGAAGTAAAGAGTTTTTATTCCTTCGTGATGAAAATATTTACTGTAATGTTTGATTTCTTCTTTTAAAGCCGAGAGGTACTGTGCGATTTGATCAGATTTTTCTGTTAGAGCAAAAGAAGAGAATGCACAGTAGTTGCATTTGGATTGGCAAAAAGGAATATGCATGTAGAGTGAAAGCATTACTGAGGATTTTAGTAAAATACTCTTGATGATCTATTTATAAAGTAAAGAAAATACTTGTCATTTGCAAGAGAAAGTTTATTTTTCAAAAAACTCTTGCATTTGCTTCCTATTTTCGTATACACAGGGTCGTTCATATTTTTTATTTCATTATGAATGGTAAAATGACAAAGAAAATGTTGGCAGTAGCTTTGATGGCTGGGTCTGTATTGGTATTGGCTGGATGTAATAAAACTCCTGCTGAAACTGAAAATGAAGTTGTATCACCTGATGCAAGCGGTGTTGTAATTGATGTAGATGCAACATCTGGAGCAACTATGGACGATTCTGCAGCTTCTAGTTTTGATGAAACTTCTCTTACTGGAGATGAATCACTAACTTCTGGTGCAGATACTGCTTCTGTTGATGAAGCTGCTAATGCTGCTCTAGCTGCTCAACAAGAAGCTCTTAAGAATGCTGGTGTTGATGCTGCTGCTGTTCAAGCTGCTCAACAAGCTGCTCTTAAGAATGCTGGTGTTGATGCTGCTGCTGTTCAAGCTGCTCAACAAGCTGCTCTTAAGGCTGCTAAATAGTTTAGTACTATAAAACGCTTGTAATCTCAAAGCAAGCAAGTATAAAGGAGAACATCTATAAAGGGTGTTCTTTTTTTTATTCCTCAAAAAAATATGCTACTCAGAGCATACTTACAGACAAAACATCAACTCCCAAGGAGAAAAATAACGCTACTCATTGATAATGGGAAAGTTTTTATTAACAAAGAAAAGGTAAATAACTATAAAGCTGAACTGCATGAGAAAGATCTGCTTGAAATTCCAGATATAAAGCTTCAAGAATATATTTTATCGGATACATCTAATACAGAAAATAAAAAACCAGATTTTATCCTTTTCAATAAACCTAAGGGATATACTTGTTCAAAGGCAGATCCACATAATCAGGTTTTTTATGATCTTTTGCCTCAAAAGTTTAGGAAATATTATTATATTGGGAGACTCGATAAGGAAAGTTGTGGATTGATGCTTTTGACTAGTAAGCCGGAACTCGTCCATCAGTTTGAGCATCCAAGCAAGGAGATTGAAAAGAGTTATCTTGTTCAGCTCGATAAGCCTTTTGATCGGAAGCTTAAAAATAGAATCTTAGCTGGAATCTCTGAATGAGGCGAATTTCTAAGGACAAAACAACTTGAAAAATCTGACTTTTGACTGTTGGAAATTACCCTTAATGAAGGGAAGAAAAGGCATATTAGGAGAATTTTTAAAGCTCTTGGCTATGTGGTGACTGATTTGCAAAGGATTCGTATCGGTCCTTATTGTCTTGGTTCTTTGCCTGAGGGGAAATGGAAGTTCCTTAATTAGTTTTTGCGGATTGTTAAAAAGAGGGACGATTCGCCCCTCTCATTTGCTACCTTTTCTCTGCTTTTGATGTAGCTTTGTTTCCTTCTCCTGCTGTTGAGGATAATTCCTGAACTGTGATCATTAATTGCTGTCTGGATCTTGGAATACTATATCTCTTTCGAGAATTTCTCCGTCCTGCTTTCGCATCCCATATATGGTTTCGGATATCCAGAAGAGTAGTATCATAGGCATTGTGACGAGGATTACTCCAATGAGAAAGAGTAATACAAGACAGCCATTTTCTTTTTTATTCTTTTCCATATATTTTTTAATTTATGTTTATTATGTACAGTGTATGGTTTTGGATGTAGAAAATCAAGTATTTTTTTGCTTGCAATTGATGTTGTTTATCTTAGGATAGGAAACTGCTTTTATTCATTAGGGAGATTCGATGTTTTGAAAATTTAGAAAGCTCTTGAAACCTGACCTGAAAACCCTTAATACAATCTCGATTTCTAAGGCTGCGATTTTGCATAATCTGGATATTATGAGAAAACTTCAGCCAAAATCTGCTTTCTTTCCTGTTTTGAAATCGAATGCTTATGGACATGGGATTTTGCAGATATTGGAAATTTTGAAGGGGCAGAATTTTCCGTATTTGGTCGTTGATAGCTTCCCTGAATATCAATTGGTGCATAATCATTCCAAGTTTGATATTTTATTGCTTGGTGAGACTTTGCCTGAAAACTATCGTTTTTTTGATCTGAGGAGAACTGCGTTTGCGGTTTATACTTTGAATGCCTTGAAGGCACTGGCTCAACTGCACAAAAAAGTCAGAATTCATCTTTTTTTTGATACTTGAATGCACAGAGAAGGGGTTCAAGAACAGAATTTACCTCAGATTTTGGCGTTTCTCAAAGAACATAAGCAGCTTGAATTGGAAGGACTGATGTCTCACTTACACAGTGCTGATACTGATGAAGCATCGATTCAAAAGCAAGTTACTTTGTTTAAAAAAATGGGAAATCTGGTTGAACAGGCAGGTTTTGCTCCCAAATGGAAACATATTTGAGCTTCTGCTGGTTTATTAAAGATACATGATGACTATTTTTCAGCTTTTAGACCAGGATTGCTCCTTTATGGTTATAGTCCGATTCCGGATATGAGGAGTAAATTGGGTTTGCAACCGGCACTTACTTTGCATTCTCGTATTGTTTCTCTGCAACATGTTCCTCAAGGAGAGTGAGTGAGCTACAATCAAAAATGGAAGGCTGAAAACCCTTGTAGAGTAGCCACAATTCCTTTTGGATATTATGAAGGTTGGCTGAGGACCTTGAATTGAAAACTTGTTTATCGTTATCATCAGAAGGATGTTCCGCAGCTTGGGGTGATTTGTATGAATTTGAGTTCTTGCCTAGCGAGTGAAGAAATGGCGGTTGGAGATGTTATAGAGCTTATTAGTCCTCAAGTTTGACTGCAGAATAGTATTGAGGTGATCGCTGAGAAGGCTGACACGATTCCCTATGAGATTTTGATCAGATTAGATAAGGGTATAAGAAGGGTTGTTATTTAATTTTTGTTTCCTATTTTTATTTTTCGTTATGGGGGTTAGATTCAGATTTTTTCTTTTGACTTGTGCATGCTTTTTTTTATACTTGGTTAATTTTTAGGCTTTATTTCGTTTTTTTTCTGATGAACGAACCAAGAGAGGAACCAGAGTCGTTTTGAAAAAATAATTATGATCAGCTTTCTCATATCATTTTTGATGCTTATGGGACTTTGCTTTCTCCGTCTAAATACAATATCTGGCAAGAGCTTGGAAAGCGCGCAAAAGCTACAAGAGAGGAGGTTTGAAGGGCTTTGGCGGCTTCAGATGATTTGCAGGAGATTTTGGATAAGATGGCCATTCCTAAGGCTTCTCAAAAGGAATTTATAGAAAAAGTGCGTGATGATATTGCGCAAATTCAACCTCATCCTGATGCGATTCCGCTTTTGAAGTATTTAAAAGAGCGCAAGTATTCGTTGGCGATAGATAGCTTCTTAATCCCTCTTTATGCTGGTCCTATCAAGGCGCATTTTTCTGATTATGTTGAAAAGTTTGTTTTTTCTTTTGAACAGCAGCATATGAAAGGAGAGAGTCAGCATTATTCTGATCTTTTACAATCTCTTAATATCCCTGCACATCAGGCTCTCTTTGTAGGAGATCATTATAGAAGGGACTATCTTGCTTCTAAAGCAGCTGGAATGAATGCCTTACACCTTTCTAATAAGTGAGGGAAGGTGGGTAAGGAGAGATTAACTACGCTTGATGAGCTCCTTACCGTGCTTAATATCTAATTTTTTTGCAAAACTATTTCGTGCAACTTTAATTGCTTGAGGAGTAATCTTTGATAATACTGCCTCTTTTTCTTCCCAAATAACGTTAAATTCTCATATTTGTTGTTCTAAATCAAATAATTCAGCGAAAAAGAAATGAGATACAGAATGTAAATTAAGATACTGCTTTTGCTCATACTTTTCAAGCTGAATGGTTATCTGCAGATTTTTAATCTTTTTAATATGAAGAAATCAGACTTCTTCTTTAATCTTTCTTTCAACTGCTTGTTCTAAGCTTTCTCCTTCTTCAATTTATCATCAAGGGACAGTAATGCCATATTGTTCTTCAATCAAAGCGAATTTTTCTTCATTCGGGTGCTTTAAAAAGCAGGCAACTCTTTCTCTCGTCTTTCGTTCCTTATCATTTTGAGGAGGCTGAATTTCTGAGACAATCGTAATTTTTTCCATTACCATAGTTCATCAATTAAAGCTTTTATCTCATCAAAATCATCATCTCCAATCAAAACAAGATCAAATGCTTCTTCATACGCTTTAAGTAGTTCTGGAGTCTCCAAAGTCAGAATTCAGATTTTGAGAAGCGTTTCATGATTACTTCCCTGTGCCATTTCAGCATCACCAAGTCCATCACCGATAAGAATTACATTTTTCCTTTTCTCAAGCTTTTCCCTCAGCTCTGGAAACTTAGAAAAGACTCATTCATCTTTATTGAGTGCATGAATTATTTCTCCCTCAATACCTTTTGCAATACCCTCTTTATCTCGGCTAAAACGGTTGATAACATAAGAAATATTAGGAAAATCGCATTGATTATGCTGCATAAAAAGCGGAATCGCATCACCACAGCCACTTGCAGAGAAAATTACGAGCGGAATTCAGTTTTCATCTAATTTTCTAAGACATTCTCCAACACCTTTCCTCAGCTGGAGATGACCATTTTGCGCAATATCTTTGAGGTCTGTAAGTCTGAGTTTTGAGGCAATCAAGAGTTCATCGTGCTTTTCTCGCCATTCCCACATCTGAGCTTTTCTATATTCCAGAGGCAAGCTAGAATCGCACTCTATAGCATGATAATGATCAAAGAGTGCATGAGCTTTCTCTGCATAATCGTCGGTCAAATGATTACCATCTCTCAAAAGTGAGATAATACTCGGTGTTTTTTCCCCATTGAAAGTCCCGTAAGTAAGCGTTCTATCAAAATCAGCAATGATAAAGCAAGACTCTTTCCCAGCCGCTTGAATAGCTTGCTTTTTCTTGGCAAAAAGTTCTAGATTTTTGATATGGACTTGCATTGACTATTCAGCTAAGCTAAAGTGAATACTCTCATTTCAAGAAACAAATTCTGAACTGATCACTCAGTCAGCTTCTTTTTCTTCTATCGTCAATAATAAGGCTTCTCACTTAAATATTTCATCAAATTCTTTCAAAATCACTTTTAAATATTCACTTGCAGTCGTATAGGTCAGCTTTACCTTTTGCTCTACTGCGAAATCCGCATCTTTTCTCATTTGGTTGAGGAAACGAGAAATCTCTCTTGCTACCCCTTCTCTCTCAAGCTCTGGAGTCAAAGTCAAGTCAAGCTCTGCGATCATATTCCCCTCCACTGCGATATTATTGGCATCAAGCCCTTCATACGCTACTTCATAATCCTCGGCACTCAAAACCCAGTTTCACCCCTGTGGAGAGAAAACTTCAATCTGTCCGTTTTCAAGCTCTCTGATATTCCCTTGTTTTCCATTTGCGATGATTTGCCCGGTATCTTTCCCAAATTTTGCAGAAAGTTGGCTTCCCAACGGTTTAAAAACTTTCACTAAAGGCTTATCCGAACTAAAAGGTCAGATTTCTTTTACATTAACTTCTTCTTTGATGATTTCGCCGTATTTTTCCAAAAGTTGCTGTTCATTGAGCATGCTCGTGATTTTAAAAGAATAAAAATCAGATTAAATATAGAAAATCGTCGGTAGAATGCAAGATTTCTTTTTTAGATTGTATCACGAGCGATTTCTTTGTAGTAAATCGTTCTTTCTTGACCTTTGATATTGCCTTTTGCAGAGTTTTGCGTTCCGATATCTGAAAGCTGAAATTTCCCAGTACTTGTCCTCTCTAGCTGAATTAAAGCTCCTCCAAGTCCGAGCTGCTGCCCGAGCCAATAACCAATACTTCTAATATAGGTTCAAGAGCCAACTTCAAGTCTGAGCTTCAAAAGAGGAAACTGATAGTCTAAAATTTCGTAAGCATAGATTTTCATTGGCTTTTCTTGGATTTCTGCAGTTCCTTTTCTTGCATCTTTATAGAGTCTTTTCCCATTCTGTTTTTTTGCACTAAAGGTCGGCAAAGGAAGCAGGACTTCTTTTTCTTCTTGAAAGAGGATTGAGTCTAAAAGCTGAGAAATCTGATCCAAACTTGGGGCAGGAATCAGTTTTCCTTCTTTTTTAAGAAAAAAAGCTGTTCAGGTATCCTGAGACTCAATGACTTCAAATCTCTCTTCCTTCTCCCAGAAACTTGCATCTCGGGTATCAGTCAGAAGCGAAAAATTAATCGTCGTTTCATAACTTTTATCAAGCCCAATCAAAGATGTTAGTTCTTTCGTGCCTTTCCCAATTCCGAGGATCATAAGTCCACTTGCCATTGGGTCTAATGTTCCGCTGTGTCAGACTTTATTTTGGAAATGTGTCCTAATGATTTTTACCACATCAAAACTGGAAATTCCGCTTGGTTTGTAGCAGGCAAGGAGCATTGAAGTCAAAAAAAGGATAAAATTTTCGTTAAATAATAGAAAAAAGCTGACTTTTTTCAAGGAGAGCAGCTTTTGTTCTTTAAAATTCTGCTTCTTATGCTTTGAAACTATTGGAGTAAAAAAGTTTCTTAAAATTTGCATTTCTGATCCTTTCTGCGTATAATAATATCGTCATCAAGAAATAAGTCCTTTATGGTTTTTATTGCTCTTGGTGGTGTTCAGGTTTTTAAGTCTTTTGATTTGATGACTTGGGCGAAGATAATGGATGTGATCTTTAAAATTCTGCAAAATGATGTTATTGGTCTCTTCCTTATGATGGCGAGTTTTGAGCTATGGGGCAACCTTATTGCTTGGAAAATTGGCTTTTGAAATAAACAAAAGTTTAAAATCAGATTTTTTCTGGCTTGGTATTCGTAAGATATAGTCCATTTCAAAGGTGGTAGAGGATTACTGCTTTTGCTTGGAAGTAAGGTAACTGGTGCTTTCAAGGGATTAGACTAGCTTTTGCTCTGGAAAGTCGTGACTATTCTTTTTGTTTGATGAGGCTTCCTCCTCAGGAATTACTGCTGTAGGATGAGAGGACTTTTAGAAAAGGATTCAGTTTTTTATCAAGGTTTAGGTGATGGAGATGGTAGCATAAAAAGAGTATTTGTTATTTGCTCTCTTTAGAGAAAGAGTAAGTCTCTAAATGTATCAAATGATGATTGAAAGCTCTTGATCGGATTTGTTTTTACTCACTATCGTAAAAGTAGATTGATCAATCTTCCTCTAAAAGATATTGCTTTTTAAGTATCTAATTCTGGCTTTAGGATTGCCTAGAGCGTTAGGTAGCTTGGGACTGGTTCCTGAGTCTTCGGTTGTGATGAAGCTTCGTTTGCAGAACATTCATTTCTGTTATTTTCAGAAATGGAGAGTGCATGATTTCGTGTATTCTCTTTTTTTTATAGAGAAAAAAATTTAAAATTAGAAAAAAAAACTGAATAGAAATGATATTCAGTCTTTGAAGCTTTTTCTATAATGTTGAGATAACCGGCGTTTGGAATCAGCTTTTTTGCTTTTTAATAGTGAGAATAGGCAGCTCTTCTTATTCATTGCTAACGCTTTGTGTGGAATCGAGGAGGGGATTGTAAATCGAAAAGAGGAGCGAAATGAGATCTCTCGTATTGAGGAGTTCGGTTTTGATGCCGAGGCTACCGAGACCTTCTTGGATAAGGGCAACTCTTGTGTTGAGCATTTTTTGCCCTTTTATGAAGTTCCTATATCTGGAAACGATGGTTTCGATGTCGTCCTTGCTATTAAGAGTATCCATAAACTTGCTTCGTCGCGGTTTTTTTACTTGGTCGAGATCAGTTTCGCTCGTGTAGTAAGGAATGATGATATAAAATTCCTTGACGAAAATAAGCCCTTGTCTCAGGTTGATCTCTTCCAAAAAAGACGCATAATTTTTTCCTTGCTCTTTTAGAGCTCCCGGGTCTAGTTTATTGATGTTTTCGTGCACGTAATTGAGATATTCTCTCAGGTCTAGGTAGGTATTACGGATCAGAAACTGTAAGGGAAACTCTAGTCCATTGAGAAATCTTTTGTATTGCTCTAAGATGGTTTGGATTTCGTCAAAGTTTTTGAGATCGAGGTTGACGCCGTCTGCCTTCAGGATTGCTCTGATTCCTCCGTCTTTGAGAATTGCGTATCCGTCCTTGATCTCAGAAATAGGAAGCATTGTCTCGGTATTAAACTGAGTTTCTTGTTTCCCTTTTTGGATATTTTGATTTTCAAGGTTTTTGAAGAACATTTTCCTACGGTTTGGCTAAAAGAATGCTGTGAAGGAACAGGAGCTACGGTGTATATGCTTTGTTTTGATAGATTCGGAAGAGGAGTGTCACGATCATTCGTGCTAAGCCCATAGCTGCGATAGCGATGGCGATTCCTTTTAGGGTGCTTTTTACGTTGTTAAATTGTTTATCTCCGTCTCCAACCACCATACCATAGAAACTATACAAAAGAAGACAAAATGCAATAAGACTTACAAAACTGAGTGCATAATTCAGAATTTTTTTGATATAGTAAAAGGCTTTCTGTGGTCCGTGATAATCTGGACCATCAAGGCTAAACACACTGAGCAATCTATTTAGCAGGCTATCTTCTGGGCTTACAGCATCGTCTTTTACGAGCTTTCCGTATTCAGTATCAGTATCTGTTCCTATGACTCCTCCTTCATAGTAAAACGGGTTCTCCTCTGCAAAAATGGGCGCAAAAGAAATGCATGCGAAAATGCTTGTTATCAAGAATTTTATTCAGAGCTTTGGGAGTTGCATCTATTTTTTTATTCACGAATATAAAGCTGAATTTTTTAGAGGTTCATTACCGTTTTTAGGAGTCGGAAGATACCACTTACGACAAATCGGCTGATTGCGATTCCTGCGATTCAGATTGCTATTTTTTTGAGGGTTTTATTTCCTGCCTTAAATTTTCCATCGTCTCCGGCGGCTGTTGTCATTTGGAAGCCAGCATAGAGTAATATGCAAAAAGCGACCAGACTAAGCATTCCCAGTGCGTAATTTATAAGTTTATGGATGAGATCAAGGGTTCTCTTGGTTGCGTCTTTTTGTGATTCGATTTTTGTTTTACTTTCAATGAGGTTGCCGATTTCTTTGTCGCTGTCTTTGGAATGAATGATGTCGTGGCTTCACTGACGCATTGCTTCTTGGTCGGGAGGAGTGATGATGTCTTTGTCTTGAGTAGTGAGGATTTGGTCCGTTGTGGGACCGAGAGATCTTGTGGCTGTAGAAAAGTTTATCCCCAAGATGAGCATACTACACAATCCTAAAATTCCATACTTTCGCATTTTTTTATGGCTATATAAATTCTCCTTAAGTATACTTAGTTCTGGAGAAAAATGCAAATTTTGGCGGTTTTTTGCCAGTGAGAAAAAGAAAAAAATTGACCTCAACGATCAGTTTTAGATGTTTTTAAAATTGTAAAACTCAATTCCATTGATCATATCGGTTTTTACGGGATTGAGAGTTTCGTTTGCCTGGAGAATGAGGATTTTATCATCAAGAGAAATGTTCCCCTTGAAGATCATTCTAATCATTTCTTTTCCGATTTTTTTGAGATTCTCGTAGTTGAAGGATTGTGAGATTTTGTATCCTTTTACTCCTCGCACGAGACTTAAGTATCTGTAGGTATCTTTTGATTGGGTAAAGGTGATGATTGGTATCTTTGGACCCAATGAAGAATATCTCGAGGCAGTATAGCCGTTTTCTGTGAAGCAAATCGTTGTTTTGATTCAGAATTCTTCTATACTTCTGAATGCATTGTAAATAATATAATCTCTTACCTCAAATTCATTACTTGCTTTGAATCTTTCAATTTCCTTGTATTCGTATTTGAGTTCGTGATTTCAGAGGATATCTCCTGCTTTTTCAATTACATCAAAAACCTGATCCTCAATAAGGAAGGTCTCTAGCATGATTCCGTCAATATGTTCCTGAGCTAATTCTTGCATCTCTTTCCCAAAAGATTTCAGAAAAGGTTTTTCGTAATCTCTATTAGTATAGGTGATAAGCACAGGTTTTCCTTCGGCTTTGACTGACTGGATCAATGTGTTTAGTTTCTTGGCTGGATTCAACTTTTCTAGTACATTGGCAACGAGGATTATTCCGTCTGCGGTTTTGAGGATTTCTTTGAAGTTCTGGAGTCAAGACGTGGTTTCTATCTTGGCGAAAACTTTCATTTCTTTCGCATTATTTTGATCAAGGAATTCTTTTGCACTTTCTACATGTTCGGCACTATTGGAGCAGGCAAGTCCGATGACGTGAGCTCCGTATTCTAGTCCCCAGAGGATGTCTTTTTTATCCTTTTCTGTTAGGGCTTCATCTTTGTTATCTAAGGCATCAAAATTTATTCTATCGTATTGCAAAATTTCTCAGTTTCCTGCTGATAAAACTTCGACTTGTGCGGTATCCTCTTCTGTGCTCAGAATTTTAAAGCTCGTGTTGCTCTGTTCACAGGTGATGATCGTATTTTCCTTCAAATTTCCGAGGGCTGGATAGTCGATGTAAATTTTACTCGTTCCTTCTTGTGCGTATTCAGAATACTCCATGGTCCATTTTTCTCCCTTTTTGATTGGAAAATTGCAGGTGTTTTTCATTCTGATATCAATCCCTTTGGTTTCCAAGATGATTGTTTTGCTGTTGTCAAGCTTCATGATGGTATCAATGTATTTTTTGTTATTGTCGTCATAGCCTTTTGAGAGGGTAATTCTAAAGGCATCTACCATATTGATGATTTTAGAAAGCACGGTTTCTTTAGCAAGGATCGGACTTACTCCAGCAATGATTTTGGTAAATCTAAAGTTGTTCATGATGAAAAAGAAGGGTAAAAATCAGAATTAATTTTTTTTGTTATTGTCGGAGAATCGGGTATTATCCTTCAGTATTGACTACGACAAAGCCTTTTCTCTTGATGAGTGTAGCAGTTTCTTTATTTTTGATGACAATGTTTGCTTGATTATCCGCCTTTGCATAGACTAATCCATCATCAACGAGCAGTGGAACCGTTTTATTTCTAAACTCTTCTGGAAGCGAAATCACCAAAGGATCGCCTTTCCTATCGCCTCCTGCAATATCTACCTTCACTTCAAGCAAAGGCAATTCATTAAAGGTTCTGACATGAATTCTGAGTCCGATATCTTTTTCAAGGTTCGTGATTGCGGTTCAGCCTTTTCCGATAATTTTTCCTTTATAGTATTCAGGAATATAAAGCTCAAGCTCGCTTCCTTTGATCGCAATATGGAAATCACAAGGGAGCAACTGCTGTAATTTTCTCTGAATTCCCTCTTTTGCATACTGAGAAACGACTTGGGTTTTGGTTGGTACTTTTGGATTGCCATTTTCATCGGTAGCAATTGGCATTACCACGACTTGCTCTCCAAAGGTATAGATTTCATAAAGTGACTTTTTCTGAAGGAAGCTAGAAACGACAATGACTGGTCTCGCAAGCTCTTCTGAAAGCATTCCATCTGGGACTTTTGCAGTAAGTTCTAGGGTTAGGACTTCACTCACTTGCCCTTTATCAATAAAGATAACGGTATCTACGACTTGAGGAATGATTCCCATTTCAATACTTCCGATGAATCTCTGAATACTATCTACCGGTTTCGTCGCATGGATGACTCCAACGAGTCCAATTCCTGTGAGTCTGAGATCCTTATAGAGTTCAAAGTCAGGTTTATTTCTTACTTCATCATAAATCGTATAATCAGGTCTAGAAAGCAGAAGAATATCCCTCACTTCATCGTGGCTTCCGTAGGTAAAGGAATACTGCACAATATCATCATTCAGCATCAAATCTCTTGGCGACTCAATCGTCTTGATGATATGCTGATCTTTGTGATATAGCTCAATCAAAGCCCCAGTAAAAGTAGATTTTCCGCTTCCAGGAGCTCCAGAAACGAGGATTCCTTTAGATTTATTCCTCAAGAGTTCAAAGAGTTCTGGCTGGAGATTATACTCTTCAATGCTCATTTTCTTTACCGGTTTCACTGCGGTAAGTTCTAGTCCATCGGAAAGTGCAGGATAGACGATTACGATTCTATATGGTCAGATTTGAAGGACTTTAGAGAGTTCTCTATTGATCTCTAAAAAAGCATCTTCTCTGCTTTCTACCTCTTTAAAAAGCTGATCAATCAGTTCATTAAAGGCTTCTTTGGTCATAATTTCATTTGGATCCACGACAGGTTGCCAATTCCCTGGGAATCCTTTTTTCCTGAGAATTGGTCTATTAGCTTTGAGATGGAGAGACATAGTCGTTTCGTCAAAATATTTGTCCAAGACTGAGTTTCCAATAATTGTTTGCGTCATATTTTTGTTTACAAGATTAAAAAGCCGTAGCCTTTCTCATTATAGTCAGATGAAGGAGAAAATACAAAAAAAACCTGACTTTTCTTTTGAGGTAAAACGGCGGAGTATATATTGATTTTTTTGTTTTTTTCAATATAAAGTGAATGTGTAGGAAAATTTTCCTAGGTTTTATCGGTATTTTTGGAAATGTTTTTTCATTTAACGGGAATAAAAGATATTGCAGATAGAAATTATAGCTTGGAGTTTCTCATTGAGGGAGAAGATATATCGTCTGTCAAGCAATTCCTTGCTGATCAGAAGGTTATTGTTCTTGGATTGGAGCATTTTACCAATGAACCTCAGACTTTTGGAGCTGTTTATTTGGATGTTGAGTCTAAGAAGAAGGTTTTTAGGATTGTTGGGAAGTTTGATACGTTAGAAGAATTTCTTGCTTACTTTGTGAAACTAAAACTTAAAGTCGTAGATGCTAATTCTTTTGTTTCTCCTCTAGAGCCTCAAAAAGTTCAATCTCTCCTTCAACAGATTTATCAGAAACAAGAAGAGCAAGAACGTCTCTATCAACAGGCCAAGGAAAAAAAGGCAAAACAGGCAAAATTAAATTTTCAAGATAAGAAGCTAGCTAGGGCATATGAGGCGATTGACCAGATTATTAGTCAAATTGATCAGCTTTTGGCTATTGGTGGGAAAAATATTCCTCCTATGACGTTGAAAAATTTTGATGATATGAGGTGAGCTATTAATAAGCTCCGTTTGGCGACAAACTATGATAAAATTATTGAAGAACTTCACAAGGCAATGAATCTTATTGTTGAAACTCAGGATTTATTACTGTGAAAACTGGATGCATGAAAGATTTTTACTGTTGTTCCGCAGAGTACTATTACGAATATCGAAGTTATTAGAGAGCAGACGAGGCTTGCTAAAGCAAAGTTGCTTGCTGCATTGGGAGCTCAGCTTTCAGGAGATGAAGTAATGTATTCTTCACTTGGGTATCTTAAAACTTTTACTCAATATCTTCGTAAGGATATTCGTACGGCGATGGATAGTAAGTTAACTTTGTGTAGATTGGTTTTTCAGGGAGCAGAATTATTGTTGTTGTTTTTCCTCTTGGAGGTTGTGATTTTTTCTGTTTTTGGTCAGAATTTTGGTCTTAATTTAGGTGTTCAAAGGGTTGGAATTATCCTGATATATGGAGGTGTTTTGGCGGGACTTTTGAGAATTGTGAATGAGTATTTTCGTCCTCAAACGGTTTTGTATTATCTGTTCTTTTCCCTCGGTATTGTTGCTATTTATTTTTTTATTATCTATCTTATCAAATTATTTTTAGTTCTCTAGTAGGTTTCTTGTGATCTATTTTATCGTCCTGCTTATATGTTGAGCGTTTATAGCGTTTTTTCTTCAGCTTGGAGTCTGGTATTTTTGATTATTCCCGCTGATTTTTTTGGTTGTTTTTTTTTCTCAAGGTATAAAGGATGTTCATTTAAAATTACTGGAGACTGATCTTTCAGAAAAGTATGGTCTTTATCTGCTTATTGGATTTATTTTTTTGTGAATCTTCGGAGTTTTGAATTTTATTGGATTGGACTATCAATCTGATTTTTTTGTTTTGCTTTTTCTTACGTTACTTGCTCGATATGGAGCCTATTTTCTAAATTATGAAGATGGGAAGCAGCTTTTTGAGCATTTTTTCTTGATTTTCCTCTTGTTGCTCCTGTGAAATAGTGTGTTTTTGGATGGAGTGATTGGAATATCAGCCATGCTGAGCTTAACTTCACTTTTTGCAGTATTGGCTTATGGAGGTTTGCGATATTGAGTTGCGTCTTTTTTCCCGGTAGATTCGAAATATCAGTATTATTTTTTCTTTTTCCTTTTTTTGGCTTTTCTCCGTGCTCTTTTCGTTAGTGTGAATGGAGGACTTCAAGCATTGAACTTGGATTTGGGAATTTGCTTTGTTTTGCTCCTTTGTTTGGATTATTCCAAGTCTTCTCCGCAGAAAAATCTTGAAGCCTCTAGGAATGTTTCACTTAGGAGGGTTTTGGCAGGGGAGAGGATTTTGGGCAAAAAAAATAGACTCACTCTGATGCACTCAGGAATTCTTTTTTTCAAGGAGTTGCCAATGAAACTTTGGAACTTTTTGGAGTATCTGAATGTAGTTCTTTTGCTAGGAGTCCTTGTTGCTTATCTCTTTCCTGTGTTTCAGGGGCAGGTTTTGCAGCAGTGGTGGTATCGATTGGGGATTGCGTTTTTCTTAGCTAATGCTTTTTTGCTGAAGAAGAATCAGATTTTTTCTCTTTTGAGTAGATTTGCTGTGGTTGTGATTATTAATTTTTCTTTGTATGTGAGTTTATTGGTCGTGGATAAGAATATACAGTCAATGCTTCCTTGGCTTATCGTTTGGAATCTGCTCTGTGGAGTGCTTGTTTTTTATACAAGATTTGGCATGGTAAGGAAATATGTGAAGAAAATTGATGTGCTTTTCTGGCTGATTGCAAGTCTCGTGGCGATGTTGCTTAATATTGTGCTTCTTATGTGACTTTCAATTTCTTGACAGCTGATTTTTTCGCTAATCTTTTTCTATTTGGGGATTCAATGAGTGATTACTTATTATGTCATTCAACTGATAAAAGTGTATGATTTACCAAAGACTGAGGAAGGATCAGCTAATTCTGACCCTCTTGAGAAACTTTTGGAGAAGGAAATTTCTCTTTAATGTAGTTATTTACTCTCTTCTGGGATAATGATAATGATTTGTTTTTTGGAGAGGTCAGAATTTTTTGGAGTATAAAGATTATCTCGGTTTTCTCTTTTTTCTCTCAGGACTTCTAATTTTAGAATATCGCTTTTTGCTTTTGCTGAGTAGAGTTCCTGATTTGCGAGTTTCATAAAATATCCTCTTGTGGTCGAAAAATTAATGTAATACATAAATATGCCTCAGAAGAGAAGAAAAACAGCGAGTAATCCTCAGATTTTTGCTAGATCTCGTAATTCTTGTTTAGTTCTGTAGGTTTCGAGGAAACTTTTGAAGCTTTTGTGAAATGATTTTAATGATTGATAGGCGAAAGTTTTTACTTTATGCTGAGCTATTTGGAAAGAGGTGTTATTCTCTGGCATTGGGCGTATTGTTGAATAAATCTGGTTTTTATTTTTTTTCAATAATTCTGAGTTTTGCACTTCTGGCGGCTTTATTTCTTTGGACTTCAGTATAGTGGGGAACAATTACTTTTTTATTTATTAGCTGAAAGTCTCAGCTTTCTGCAAGGGTTTTGAAGGCATTTTTGGTGATTCTGTCCTCAATACTATGATAGGTAATAATCAAGCATCTTCAACCCTTTTTCAAAGTCTGAGGAAAGTCTTCTAAAAACTGCTCTAACTGTTCAAGTTCACGATTGACCATAATTCTGATGACTTGGAAAATGACGGCAATCTTTTTCTGATTGAAATGATGGCTGGTAAGAAAGTCTTTGAGCTGAAAAGTAGTTGAAAATTCAGATTTTTTTCTTTGTTCACAGAGGAGTTTGGTGAGCCATTCAGCGGTTTTGGGAGAGAAGTCTCCATAGATTTGTAAGGCTTGTTCAATGGTTTCGGGTTTTGTTTTTTGAAGCCAATCTTTTGCGGTCGTCGGATTCTTTTCCTGATCAAATCTCATATCCAGCGGTGCATCGGTTTTGATCGAAAATCCACGGCTTCCATCCTTGAAATGTTCTAAGTTTACACCAAGGTCTAGAAATTCGTAGTCGAAGAGTCAGTTTTCTTTGCTAATATCCTTAATTTTGGCATATGAAGTATGGAGAATTTTGATTTGATGAGCGTAGGTTTTGGTTAGCTCTATCGCTTTCTGGATCATCTGCTGGTCAATATCAGTTCCGATGATTTTTAATGTTTCAATCGGTCTTTTCTGCTCTTCTTGGCTGAGGAAAAACTCCGCATGTCCTCCATGTCCAAAAGTTCCATCAAAGCCAAGTTGTCGGTTTGGTGGGAGCAGATCGTAGATTTCTTGGGCGAGGACAGGAATATGGCGGAGCATAACAAAGCAGAAATATAAACAGGTCTGAGCATACGGAAATAGAGGAGAAAAACAATCAGATTTCTCTTTAAGAGGATTATTAGAAGACGGCAGGCTTTTTTACTTTTCTCCCTGATATTGGTAGGAAAGGATATTATATTCTTCCGTATCATAAGCGACAATCCCTTCTGGATGCCAGAGATTAAAGGTTTTTATTCCTACTTCCAAAGCCATTTTACTGCAAATCGTGCAATAGGGGTTGCCGGATTTAGCAGGTTTCCCTTCTGGATCTAGGCGAATAAAATATAAATCTGAACCTATAAGTTTATCTGAAGCGAATTTCAAAGCATCGAGAATTGCTCTTTGTTCAGCATGGATGCAGCATGTCTTATCAGTAACTTTTCTATGATACTGTTCCTTATCGCAGTGGCATCTTCTCTGGGATTCTAAGTGCTGAGGTGGGGAGTTGAAGCCTTGCCCGATGATTTCTCCGTCTTTGACGATGACACAGCCACACTTGGATCTTTCACAAGTCGCTTTTTGTGCTATTTTTGCGGCTTGTTGGATAAAAAACTCTGCTTGTGTATGATTTTGTCAGGTTAGAAGCTTCATTTTTATTTTTCTAAGGTTAAAAGTTATTCTAAAAAGGTTTTATAATCAATCCATTTTGCATGCATTTGGTAAGATTTTTATTTTTTACGATGGCATATTAGCATTTGGATCTTTTATATTTTAAGCGAGTCTACAGAAATAGAGGAGAAAAACAATCAGATTTTTTTACTTAAAAAAACACTCCAACCGTTAATCGTTGAAGTGCTTTTCGTTGTATTTAAGAAATAAGAGTTCAATAAGATTATTCTTCATCCATTTTCTTTACCTTTCCTGCTCTTTCTTCGATGATTTTCTTTTCAATCATTGCAGGAACCTTTTCATAGTTTGAGAACTCCATAGAGTATGCAGCTCTTCCTTGAGTATTTGATCTCAAATCAGTCGCATATCCAAACATCTCTGAAAGTGGAACTTTTGCAGCAATTGCTGTAGCATTTCCTCTTGGAGTTTGTCCGAGGATGATACCTCTCCTAGATGAAAGATCTCCCATTACGGTTCCTACGTATTCTTCTGGAGTTACGATTTCTACAGACATGATTGGTTCAAGAATGGCAGGAGATGCTTTTGCGAAAGCCTCTTTGAAAGCCTTATAAGTGGCAACTTTAAACGCAACTTCTGAAGAATCCACATCATGATAAGATCCAAAGAAAGGAACTACCTTAATATTGATGATAGGGAATCCAGCAAGGATACCTTGAGCCATGGTTTCTTTTGCTCCCTTATCAATAGCAGGGATAAACTCGTTAGGGATTACTCCACCTTTAATTTCTGATACAAATTCGTAGTTTTTCTCTTCTGGAAGTTTTTCTAGTCTAAGAACTACGTGTCCATACTGACCTCTACCTCCAGATTGTTTCTTGAAGAGTCCTTCTCCTTCTGCAGTAGCGAAAATAGTTTCTCTATACGCTACTTGAGGTTTACCAGTATTTACTTCTACTTTATGCTCTCTCTTGAGTCTATCGACGATCACATCAAGATGAAGCTCACCCATACCTCCGATCACAGTTTGCCCACTTTCTTCATCAGAATGAGCAGTGAAAGAAGGATCTTCAGCCATAAGTTTTGAAAGAGCCAAACCAAGCTTTTCTTGATCAGCTTTTGATTTTGGTTCAATAGCAATATGGATCACTGGATCAGGGAATTCCATTTTTTCAAGAACGATAGGTTTTGCAGCATCACAAAGCGTGTGTCCTGTTTGAGTTTCTTTAAGTCCGAGGAAAGCACAGATATTTCCAGCTGAAATTTCAGAAATTTCTTCTCTCTTGTTTGCGTGCATCAGAAGAAGTCTTCCTACTCTTTCTTTCTTTCCAGTAATAGAATTAAGCAAAGTATCTCATGACTTGATAGTTCCTGAGTATACTCTCACGAAAGTCAAAGTCCCTACAAAAGGATCAGTCATGATCTTGAAAGCAATAGCAGAAACAGGCTCATTAGGGTCGGCTCTCCTTACTACTACTTTTTCTTCATTATCAGGATCATGTCCAACTATTTCCCCTCTATCAAGTGGAGAAGGCAAGAAATCTACTACTGCATTGAGTACAAGTTGAACTCCTTTATTTCCAAGTGCAGTTCCACAAAGCATAGGATAAAGCTGATTGCTAATAGTTCCTGTTCTAATTGCTTTTTTGATAAGATCGATACTGATCTCTTCTCCTCCTAGGAATTTTTCAGCGAGTTCATCATCAAACATTGAGATTGTATCAATCATTTTTTCTCTCCATTCTTCAGCTTGTGCTTGCATATTGGCTGGGATTTCTCCTTCAACAACATCTACTCCTTTTTCTCCTTCAAAATGATAGGCTTTCATTGTAATAAGGTCAATAATTCCCTCAAAAGTATCAGCCTTTCCAATAGGGAGCTGAATAGGAACTACCTTATCGCTCAGTCTTTTTTCTACTGATTCAAGAGACATGAAGAAGTCTGCTCCCATTTTATCCATCTTATTTGCGAAGATGAGTCTAGGAACTCCATATTTATCAGCTTGTCTCCAAACCGTTTCTGTCTGAGGTTCTGCTCCTTGAGAAACATCAAGCAAAGCAACAGCACCATCAAGCACTCTAAGAGATCTCTCTACTTCTACCGTAAAATCTACGTGACCTGGGGTATCAATCACATTTACCTGGATGTCGTGCCAAAAACATGTCGTAGCAGCAGAGGTAATTGTAATTCCTCTTTCTTTTTCCTGCTCCATCCAATCCATATCAGCTTCTCCATCGTGAGTTTCTCCAATCTTGTGCTTCTTCCCTGTGTAATACAAAATTCTCTCAGTTGTTGTGGTTTTACCAGCATCAATATGCGCCATGATACCGATATTCCTTACCTTATCCAAAGGTTTTTTGTCGTTTGCCATCAGTATAAAACTTTAAAATAAAATAATTGGCTTTGCCGTTTTCTTCAAATCTCTGAGAAAAATCCCAAAAATTCAAAAAAATATGACCATCGTTACTATAAAAAAACGCCTGCTGTTTTCAAGTGTTTTTTATCTCGGGAGAAAAGGTTAAAAAAAATTAAAATCAGATTTTTTATCTCTTTTTTTTCTTCTTTTCCATAATCAGAGAAATGATTAGCGAGAGGACGAGGATATTGATCAAGATAATCAGTGAGGCAGTAGAAGAGAAATGAATAACAGGACTCAGAAGCATTTTTATTCCAATAAAGGTGAGAATTACTGCTATTCCATACTTTAAATAGGCGAATTTATCAATAAAGTTCGCTAATAGAAAATAGAGGCTTCTTAATCCGAGAATTGCAAAAATGTTGGAAGCGTAGAGGATAAAGGGATCGTTTGGAGCGATCGCAAAAATTGCAGGAATGCTATCTACGGCAAAAAGTAAATCTGAAAACTCGACCACACCTACGACGAGTAATAGAGGAGTGGCGTATTTTAGTCCGTTTTTTAGCACAAAGAAATCTGATCCGTGAAACTCATCAGTCATCCTTGGGAAAATTAGCTTTACGATTTTTGCTCCTAAAGTGTCAGAGAAATTCTGTTTTTCTGCTGCTTGTTCTTTCTGAAAAAATGACTTTATCCCAGCATAGATCAGAAATATTCAGAATAGAGAAAGTAAAATATTGATCTTTATTGAGAAATTTCTTATGGAAAATTCTGGAAGATAGGTAAGCTGGATAAGTTTTACTCCCGTAAAAATAAAAATTGCTCTAAAAACCAAAGCTCAAATGACTCCTCGAAATAGCACTTTATGATGAGCCTTTTTCTCAACACGAAAAAAACTAAAAATCATCACAAAGACAAAGAGATTATCTACAGAGAGTAATTTTTCTATCCAATACGCAGAAAGGAACTGTGAAAATCTTTCTACTCCCGAATCTCGATAGACATATCCTGCGAAAAGCATTGAAAGTCCGATCCAAACAAGTGTTTGTATAAGTGCTTCTTTTGTCTTCACTTCGTGACTTTTTTTGTTGAAGAGTCCTAAGTCAAGCAGTATCATAGCCGAGACTATGACTCAAAATACAATCATTTCGTGGGTATACATCGAATATTTGTATGAGTAAAATGGTAGCAAACTATAGCTATTCTTTTTTCTATTTCCATAAAAAAGTAGAAAATCTGATTAAAAATTCAGTATTTTCTTTCGCCTTTTGACTAAAATTTCCTGCTTTATAACTGCTATTTTTCTCTTCTTTGTAAAAAGTCTTCGAATACTTGACAAATATTATTTTTGTGTTATAATAATGTTGTCTTTATTTTTTTATAAAAATACACCCATGAAAAAACTAGTAAAAAAACTCCACAAAGCTGAGGCTGAAAGAACAAAGGTTCAGAAGATTGTTAGAGATGTAGCTTTCTTTTGAGTTGTCGTGTATGCAGCAGTGATGACATTTATGGTCTTTACTGCAGAAGCAGATAAAACTGAGGCTGAACTTGGTGTTGTATCAACAACTATTGAAGCTTCACACGTTGCTCTTGCAAGATAATTTGGAGATATACTAGAAAAAAGTCTGAATGGGGATGCTTATTCAGATTTTTTATTTTTTTTAATATTGTTGCAGGCTTTTTTCGTCGTCAATTGGATCTAGTTTGATGAGTACATTTGCTTTTTTAAATTTCTTTTTGATCGAAGTTTCGACGTCATTGTTTACTTGGTGGGCTTCTTTGAGGCTAATCCCCGCGTTTTTAAACACCATATGAAAGCTGATAAATGCTCGGTTTCCTGATTGGTGGGTCCTGAGTTCGTGGAAGCTTTTTACGGCAGGATGGGCTAGAATGATTGTTCCAATTTCATGGTCTTTACCTAAACTTGTGTCCATTAAGAGGTCAATTCCTTTTTTTGCAATGCTTCGTGCACTATAGATCATGTAAAGTGCCATTCCACTCGCAATAATTGGATCCATCCAAAACCATTGAGTATGTTGTATAATGATTAGGGTAATGATGATTCAAATATTGGTAAGGATATCCATCTCATAGTGCATTAAATCTGCCTGAATAATCAGACTTTTATCCTTTTTGTTTACCTTTCTGAGTAAAAAAATGATGATTCCAGTAATGAAAATCGCAATTCCCATTGCAATAATTCATGCTCATGTTTCTTCCAGTGGAGTCTTATTAAGATATTTCTGTATCGATGAATAGATCAAAATAATTCCAGAGCCTCAGATGAAAACGCTTTCTGCAAGGGCTACGATTGCTTCGATTTTTCCGTGTCCGTAATTGTGTTCTTTATCTGCTGGTTTTTCTGCTGCTTTTAACGCGAAGAGATTGAGCATTGAGATGATTAGGTCCATTACGGAATCTACTGCAGATGCGATGATTACCATACTTCAAGAAATTATTCAAAGTGTTAATTTTATGACTGCTAGTAGAGCTGCACCAAGGGAGGCGATACGGATAACTTTTTTTGCTGATAGCATTCTTTTTTGAAAGTAAAGGATGGAACTATAGCTATTTAAGTTTTTACTTCAATAGAAATTAGAGATTCAGGTTTTGGGCTGATGGAATTTTAGGAATGTTTGCTTTATTCTGGACAAATTGTCATTTCTTTCTCAGAATTATAGAGATTGATGAGATTATCTTGTTCGACTTCTTTGCTAAGGAGATTTTTGATGATGGAAAAAGTCCTCTGAGGAGATGCTTGGTCTATCTCTTCACTATTCGTTTCTAGAGCTTGTATTGGAGTGTCTTGGAGAAGGCAGAAGCTGGATTCTATTCCTATTCAAAGATCACGATAAATTTGTCAGACACAAGTATCTAATTTGCCACTTTTATTGCCAATTGAAGTGACGAAGATGATTTTTTTGGAGAAAGGGATATGGTCGTCGTTTCCAAATCAGTTTTCGCTTAGGAGAAATTTTTTATCGTCTGGAAAGCTATTTTTGAGGTAGTTTTCTCGTACTTTATAGCCTTTTTTTTGGAATCTTTTTTCAAAAGAGAAAATGAGATCATACATTTCTTCTATCTTAACATGGGTGATGACTATTTGGGGTTTTATTCAGGTTGCAGTGGAGATTTTTTTTAACATTAGTTCAATATGATCAATACATGGAAGTCCGTTCTCGACTAGAGGCTGATTTTCAATGATATTTCGTGCATTGATACAGACTAAAATCTCAGAATCCTGTCTGAATTCTGAAACTATTTTTTTCTTGTTATCTAGATCGAAACCTGGAATTTCTGTTGTGGTAGAATTGTTTTTGAGTAATTCCTCACCGATTTCAACGTAGAGTTTTCCTCTGGTTTTTGTTACTTTCTCTTTGAGGTTTGAGATTGCGGATTCAAGATATTTTTTGTTATCAAAACTACTTCCCATAGTGTCATTGAAGAGTAAAAGTTTCTTCATTATTATAGTTTTTCTTTGTATGTTTGTCAAAATATCTTGTATTTTCCTTTAATACATCCTTTACAAAATATGAAATTAGTCTGTTTTTTAAATTCTTTTTGAAAAAAGGAAAAAGTTTCTGTTTCTTATTCTTATGAAAGTCTGAAATAGCGGTTTGTTTTGGAAATGAAATGAATCTTTCGAAAACTGTTCTCTTTGGTTTTTTCTGATTTTAAACCTTTTATGCTGAGAATTGCGTTTTGAGGAGGAATTTCGTGAAAATTAAGGGCTTTTTTTGAATGTTCCGTAGATATTTAGACTAATTTTTTGGGCTGAAAATCCAGCAGGAATCCCTTCCACTTTTACTACCGGAAGGTCAATGTCGTAGATTTCTCCAGATTCAGTATCAATAAGATGGGCGTGTTCTTTTGCGGTGGATTCATAATAGCTTCTTCCTCCTAAAACTATTGCTTCTTTGAGCTCTCCTGAGTCTATGAGTTCATTGATGTTTCTATAGACCGTGGAGAGTGAGATATTGGGAAAATATTTTTTAAGTTCAAAAAAAATCTGTTCTGTGTGGCGATGTTGACCATCACAGACTTTGAGGATGATTTTTTTGTATTTAAAAACTTTTGGCATTGTTTGTAGTTTATTGAGCAGAATGTTTGGTATCATATATTTTTGGTTACTCGAAATCAAGATGAGCTTGATATTTCTTATCAGATTTTTTAACTTTCTCTTGTAAACCCCTTTCACTTACCTATAAAAAGAGCAATTTATCTTTTTTTGAAGAGAAGCTATGGCGACAAATCTAGAGCGTATGAATGAAATCCTTGATTTCTGGATGAAAGATAAGACTTTCCAGAGATCTATTGATGAAAGAGCAGAGGCAATGAGCTATAAATTCTTTGATGGTCCTCCTTTTGCGAGTGGAAGTCCACACTATGGGCATCTTTTGGCTGGAAGTATCAAGGATGTGATCCCAAGGTATAGAACCATGAGAGGGTATAAAGTAAAGAGAAAGCGAGGTTGGGACTGTCATGGTCTCCCTGTAGAAAAAGCAGTAGAGAAGGCACTTTGAATTGATGGGAAAAAGGACATTGAAGATAAAATCTGAATTGAAGCCTTTGTAGAAAAATGCCGTGCCTATGTGAATCAAACGAACGCTGATTGGAAGCGGTTTGTGGATCATACTGGACGCTGGGCTGATATTGAGAATCCGTATTTCACGATGGATTTGGATTTTATGGAAAGCGTGATCTGGTGTTTCTCTAGCATTTACAATCAAAATAAGGTCTATAAAGGGTTTAAGGTGCAGGGATACTGCCCAAGCTGTGCAACTCCGCTTGCCAACAACGAAATCTCTGATGGCTATGAAGACAAGCAAGATATGGCAATCACTATCAAGTTTGCTCATCAAGCTCCAAAAAGTAGTGCATACGAAGCTTCTGAAGATGGTTTTATTGATGTAGTAGCAGGAGTGATCAGAGATGGCGAGGGTCGCTATGCGATGATTCATCACATGAAGGAGAATCTCTGGTTTTTCCCTGGTGGAAAAGTTGAAAAAGGAGAATGCCGAATCGCAGCACTTAAGAGAGAACTCAAAGAAGAAATCGGAGTTGAAGCTGAAGAAAAACACTATCTCGGAGCGATCAAAATCATTCATCTCGGGAAACCTTATAGAGTCCATTGGTTTGAGGTAACAACCAGCGATACGCCAAGCGTTCAGGAAAGCGAAAAGCACAATGCACTCGTTTGGGTAAAAGAAGAAAAGTCTGAAAATAGCCTCGGATTTGCCCTTAATATCAACGGAACAATTATTGATGATGAAAAGGAACTTACGCATGATTTCATTGATTTTCATCTTTTCAAAAAAGTTTTGGATCCTGAGGATAAAGGCTTGATTGAGGGGAAATTCAATTTCCTTGCTTGGACGACGACGCCTTGGACTTTGCCGAGCAATATGTTTCTCGCGGTCGGAGCTGAGATTGACTATGTGACCGTTTACGATCCTGAGAGCCAAGAATACTATATTATGGCGGAAAATCTGTTGAAAAAATACTACAAATCCCCAGACCAATACAAATTTATCTACAAGCAAAAAGGAAAAGAGCTTAAAAATCTGACCTATCAACCGCTCTTTGACTATATTCCAAATTCCAAGATTCCAACTGAATACAAGAGTCAGTTTTTCCAAATTTTGAATGGAGATTTCGTAAGTACTGAGGATGGAACTGGAATCGTGCATATCGCACCGACCTTTGGAGAAATAGACTTTATGGCGGTAGCGGAACTCCTCGGTGAACAGCATGCTTTGGAGTGGCTCTTTATGCCAGTTGATGAGTATGGTTGCTTTGATGAGCAAGTGCCTGACTATCAAGGGATGAAGGTCTTTGATGCCAATAAGCTCGTGATGGATGAACTCAAGGCGAGAAAAGTGACGGTGAAAGCGGAATCTATAACCCACTCCTATCCTCACTGTCGAAGATGCCATTCTCCATTGATCTACAAGGCAATGTCAAGTTGGTTTATCAAGGAGAAAGAAATGAATGCTCAGACCTACCAACAAGCCGAGCAGATCAATTTCGTGCCTGAGACCGTAAAAAACAGATTCGTGAATGGACTTCAGCAGGCTCCTGACTGGAATATCGCGAGAAATAGATACCGAGGTTCTCCTTTGCCGATTTGGCAGAATGTGGAAGATCCTGAAGATCGCTTCTCGCTTGGGACGCTAGATGAGCTTTATCATCTCACCAAAACAGGATCAAAAAATCTGACCAAAAATCTCTTTATCCGCCACGGAAGGACAGATTTTAACGATGAGAAAAAGTTTGATGGACTTGGGGTTTCTGTCTTGACTCCAGAAGGACAAACGCAAGCCAACGCACTTGTTGAGAAGCTTGCAAAGCTAAAAGAAGAAAAAGCTGATCTCGTTTTCGTCGTTTCTCCTCTTCCAAGAGCGTGGGAAACCCTGAAACCGAGTCTGATTTCTTTCTTTGATGAAACGCTTGTTGCTGACTGTGAAAAGAAATACTTTGAAATCGTAAAGAGCTATCAAGAAGCTTTCAATAAGGGAACTTTGCTTGAAATCGTGCAGGGAAGCGATGAGCAAAATGTTTTCCAGCTTCACGAACAGATTTTCGTGGATTGGAGGATGACGGATCACCTTTCTTTTGCAGACCAAGGGAAAGTTCAGCCTTGTGATCTTTTGAACTGGCAAGATGCAAGCAAATCGATCGGAACTGATGGTGAAACGAACGAAACTATGTTTGTGAGAGTAAAAAATGCGGTGCAGTATTGGAATGCAAAGTTTGCGACAAAAACGCTTATCTACGCTTCTCACAACGATACGATTGCGATGGCGAGAAAAGCTTTCCGCAACTTTGATTATGCGAGCAAGAGAAAGCAGTTCTTGACTAAAAATGGAGAAATTCAGACGCATTACCGAGATAATGCGAGAAATACCGAAGTGGATCTTCATAAGCCGTATGTGGATTCTTACTGGGGAATGAGAGAAGGTAAGACCTACAAGAGAATCCCTGAAGTAATGGATTGCTGGTTTGAATCAGGTTCTATGCCGTTTGGACAGGATCATTATTTGGGGGAAGAAAAAGCTGAACTCACCTATCCAGCCGACTTTATCGCTGAAGGACTAGATCAAACCAGAGGTTGGTTCCGTTCTCTCCATGTCGTAGGACACGCAATCAAAGGAGAAAATGCCTTCAAAAATGTGATCGTAAACGGACTAATTCTCGCAGAAGATGGAAAGAAAATGGCGAAAAGTCTGAAGAATTATCCAGATCCTAGAGGACTCATTGAAAAACGAGGAGGGGATGCTTTCAGACTCTATACTTTGAGTGCTCCTGTTGTAAGAGCTGAACCGATGAGATTCGCTGAAAAAGGAGTAGAACAATGCTTCAAAGATTTCAATATTCCACTAGAAAATGTCTTCAAGTTCTTTGAAACCTACGCAAAGATTGATAAGCGAAAGAGTGATGGAACTGAGGTTTTCTTGATGAGGCATGCAAAAGCCGAAGGGCAGGGCATGAATGATCTACTTACTGCAGAAGGGAAAGCTGAACTTGAAAGTCAGGAAACGAGAGAAAAAATCATCAGACTTAATCCTGATATTATCGTTCATACACCATTTTTGAGAACGAAAACTACTGCCGAATATGTGCAGCAAACCCTCAAAACCCTTACAGGAAGAGAAATACCGCTTCAAGTCATCAATCCACTTGGTGATGACAACGCAAAAAAATCCTATATGAAAGCCCTCTATGATGATCTCTTGAAAGAACATCAAGGGAAAAGAATCTTGATCATAGGTCATGCCTATACTTTGAGAACGATCCGAGAAATTCTTTATGAAAACTATTTTGAAGATTATGCTTATACCGAAGTTCAAAGACAAGAAGGACTCAAGCAGCTTGAAATCGTTAAACTTCCGACCTATCAGATTGCAAATGAGCTTGATAAGTGGATTCTCGCAGAGCTTCATCAGACTTTGAAGCTTGTAGATCACTACCTTGAGGCTTGTGAACTTGATGCTGCTCTCAAAAGCGGTATGGAATTCGTTGAGAAGCTTACCAACTGGTATCTTAGAAGAAGCAGGAGAAGATTCCGAGGAAGTGAGATGACAACTGATAAACATTCAGCATACGCTACTCTCTTTGAGGTCTTGAGGACCTATCTCCAGATGATGGCTCCATTTACGCCGTTCATCACTGAGGAACTCTGGCAGAAACTTCAGACTTTTGTTGCTGGTAAAGAAGGAGAAAAAGCTGAATCGCTCCACCTTGCTTATTGGCCATTCGCGAGTGAAAAATACATTGATCAGCAGCTTATGGAAGAAATTGCAACCGTGAGAAAAGCGATCAAGCTTGCCCTCTTCATCAGATCAAAGAATAAAATCGCGGTTAAGCAACCTCTGCAAAAGCTGAGCATTAAGCTGTAAATGATAGTTAAGCTTTTTTATCCCTCAAGTGCTAAACCATGAAAAGAAACTGAATCTTGTGAATTATCATCAGTCTTTCCCTCATTTGTTGAGTGAGCTCAGCTTACTTTCCTTATAAAAGTGAGTGGAATTGTAGTAAAATTGAGGATAAAGAAGCTAGAGAAATGTGCGAAAACGAAATGTCTTTTGCCAAATTTACCGAAACAACAGGGACAAGAGAAATGTCTACCACTTGCTGTAGCTATTTAGACCATGATCCTAAGTTCAACCTACGGCGAGAACAGGTAACAGCATTTGGAGGACTTGATAAACGGACAAACGAAGCTTCTGAAGAAATAAAAAATAAACCCGATCAGGATCGAGCCCTTATCTCAGGTTCTGTCCAACAACTCACAGATCTTATTCTTAAGTTAAGCACTCAGATTTACGAAGAAGCTTCAAATAAAATTCAGGCTACTAAAATGCTATCTTCGCTCTTCAAAGGTTGTAGTGAAAATAATAAGAACCTTGAAGCGAGAATCGTTTGTAAATACTTCGCCTATGATCTTATTTGAAAGGAGCCTAAAATCACTCCCATAAGCTTATCTGATATTACTACAGCTTTTCAAAAAGAAAAAAAGCTGATCCAGAGATCGGTTCAATTCAGCGACTTAAACGAGAATTACACTCCAATCAATTATCATTATGCTACCATCCGTAAAATGTATCGTATCGGAGGTTTTGCTTTCTGACTTATGGACTCTTATGATTACTTTGATAAAGATTCAGATACTGATTTCTTTAAAATTTTAGTTTTGAAAGCTTGAGATACGCATTGGCAAGATTTTGCGGATATTAAGGTAAATGCCAATTTTACTGATGAACTAGCTTTAACCTTTGAAAGTCATAAGTTGGGTCTCACTATTCCTGTCCTTAAAAATAAGGAAAAAGAAAACGAAAGGCTGACTGCTCAATTTCAGATGCAAAGAGATTGAGTCCGAAAATTAGTAGGCTGTTTTGAGGAGAAATTTAATGGGACTCAGGCTACTACTGAGGATCTCATCTCTAGAAAGAAACTTCCTCTTAAGTCTTGCGAAGCTCTGGGAATGGAAGTTACAACTACTTTATGAAAGTAACTCCTCTACAACAAATCATTTCTCAGTATACCTGTAAGCTTTTCTTTTAAGGAAGAGGGAAGATGATCTTCTCTTTTTCTTTTTGTTCAAGGGGGAATGATTGGATCACTCGTTGCCCTTTTAGAGAAAAGTTATTTGACTTTTTTCTCGTAGAGGGGTTAATGAAAAAGCTTTTTTCTTAGTAGAAAGATAAAGAGAAATAATTTGGTAGTTGATAATTTCTTTCAAATAAATGAAAAAGTCTAAAAATAAACTTGAATTTCACTCTTAAAAGTATATGGTGGCGGACACATGAAAATAAAAAACCTATGTAAACAAAAATTATCTATGAAGTTTGCCTTGCGATAGCAAGCTGAACTTCCGAAGTCATAGAGAGGACTTTGCTACTCATGCCCTCTAAAAGGTAGGAAGTGGGCTACCTTAATCAACAAGAAAGCGAATATCGCCACTTCGGTATTTGCAGGAAACAAAAAATAAAAAATAAATTGGTATGACTGCTATTAAGAATCTAATGTATGTATTGATTATCGGCGTTTTCTCTGTTTCGTGTGATAAGAATGCAGAACTTCTAAGTTCTGACCCAATAGAACATTTTGTTCTGCATCAGGAATCCTCCTTTGATATTCAAAGGAAAATTGATTATCTGTGTTCCATGTATGGTTTTAAGGAGTCTTCTGTCCAACTTCGAGATAGTCTGTTAATGGTAGAGGGAGATATAGCTTTTCATTTAAACGGTTTTTGGGAGAAATATGCTGATCGATATGAAGATCAGGTACAGTTGAGATCCCATTATGCTCGCAGAGGGCTTGTTGCATCTCAGTATAGGACAATTCTTCTGAGTGTTCCTTCGGCAGGAGATCGATATGCACTACCTACAAGCTGGCTTACCGCTTTGATTTATGCTGCTGAAGCATGGAATGGTCTTAATGGCCCTATCCGGTTCACTTTTTATAGGGGAGATCCGTATACATTTACTGTAAATGTTATGTATGTCTTTGGTAGTGATCCTGAAACTTTAACTTGGGCTGATTATCCTGGAGGTGGAAAACCTGGGAGTTATGTTTCTATTAATTCTAATGGTTTTGATGGATCTACAAGACCTCTCAATAGTAGAACTGCTGTTTTGATTCATGAATTGGGACACGTGATTGGTTTTTCGCATACAGATGAGGTTCCGGCCAATGGTTATTCTTATCTTTTAGATACTGGAGATAATAATCTTGAGGATCCAACTTCTGTCATGTGGCATCATGCACTGTCAAATTCGCAACCGCCTTATTTTTCTGCTTACGATATAGAAGCTTACAGATATTTGTATGGCTTTTAGTCGGTAAGCTGTTTGGTAACTCTTGAATTTTTTGAAAGCTTTCATATATTTATGAAGGCTTTCTTTTTTATTTTGATTTTTGTATGATGAATAAAAAAAAATTTCTATGACTTATTTTTGGGCTTTTGCTTCTTTTTTCAGATTGTAATGCTCTTTCATTGGCTAGTAATGATATTAAAGAGTATTTAGAAGATCAAATTTTGTCTCATCACTCTATTAATATTCCAGTTTGCAATGATGCTCAAAATGTTCACAGGTTGTTTTCTACCTTGAAGCAGGGTGATTTCTTTGATGTTGAAAATTATGGAAATAAAAAATATTTTAATATTCTTTTGGATTTTGTGCTTAACAATCATCCAACAAATTTATTAATGAGATCTTTATTTCCGTATCTTGATTCCAAATATATAGAGAATGGGGAATGACTTTCTCTCTTCTGTAATGAGGGAGATGGCTTCTTTCACGCAGGATGATTTCAGGTAAGATGAACGATTTATCTCAAAAAAGAAAATATTGATATTATTTATAGTGGTCATTCGACATCAACATCTAGACCAACAGAAAAAACTGAAATCTATTTTCAAATTAAAGATGCTCTTGATCCTTTGGTTAAAAAATTGTCCTATAATAAGGATGGAGTATTGTTTCTTGGATGTTCTACTAATTATCAATCTCAATACATTACTCCGCACTTCGCAAGAATTAGAAGAAGGTTAGGAGATAAAAACTATAGTTATTATGTTGATGACCGTTTTTATGTTAAAACTCCTTATTTAGAGCTTACACAATATGATGGGAAAGATATTACCCTTAGATTCTATGTGGATCCATTTTCTTTTCAATCAAATTCTAACTTAGAATGCGAGAATTCACGAATGTTAAAATATGAGATTGTATCGGAAAATGAGAAAGCATAAATTTTTACTCTTTCTCTTTATAGTTTTTTTCTCCTTTTCTTGTTTTTTTCCCTCCAATAGAAGTATATCGTTTTTTGATATCGTATAATATCCGGGGTGCTGGGAATGGAGTAGCTATAATTTTTAGGTCGATGATTGCTAGTTTATTGGTAAAAAATAAATCTACAACTTCCTCTTGAAAAGTTGGCAGATATTCCTAGACTTCTAGTGGCTTTATTTTCTGACCAACTATTCACTAAGGAAACTGCGACTGTAGATTCTACCGTTTCTGATCCTGATGTTCTCGCAGTGTTTAGTGATCTTACGAAAGTATTAGCAAAACATTTAGTAGAAGGAAGGATTTTAAAGTTTGGAGATTTTGGAAACTTTCAGCTAACACTCAAAAGCGAATGAGTAGAAAGTGCAGAGAAATTCATAGCTAGCCATATTATAGGGAATAAGATTCAGTTCTGTCCTTGAACATATCTTAGAGATATGCTTGCTATGCTAAAGTATGAAAAGTATACAAAATAAAGACTTTTTTCTTCTCTTTTTTCATATCTCTCGATACTGCTAGGAGGAGTTTCTTTACTTGCTTTTTTTGATTTATTCTTCTTAATTGATATTTATGCTTCAAATCAAAAATCTTTCCGTGAAGGTCGGAGAAAAACAAATTCTAAATCAGATTTCTTTTGACTTTGAAACTGGGAAAAACTACTGTATCCTTGGGAAAAATGGCTCTGGGAAGTCCTCACTTGCGATGACCCTTGCTGGAAATCCCAAATATGAAATCAGCGAAGGCGAACTCCTTCTTGATGGACAGAATCTCACAGAAATGACTCCTGACGAGAGGAATAAAGCTGGAATTTTCCTGACTTTTCAAAATATCCCTGAGATCCCTGGGGTGAAATTGTTTGAGTTTCTCAAGACGATTTATGATGCGAGAGCTGAGAAACCAAGCACTTTTTTGGAGTTCAAAAAGATTATTGAGCCGCTGATGGCGGAGCTAGAGATTGAAAAAGCTTTTCTTTGGAGGGACTTGAATGTAGGGTTTAGTGGTGGAGAAAAAAGGAAAATTGAAGTCCTTCAGATTAAGCTTCTCAAGCCGAAATATATCATTCTTGATGAGATAGATAGCGGACTTGATGTGAATGCCGTGAGGCAGGTTGCTGAGCTTTTGAAAGCGGTAAATACGGAGGAAAATTCCTTTATTGTGATTACGCATCTTTTTGAAATGCTCCAAGAGCTTCCACTAGAAAAAATTCTGATCCTTGAGCAAGGAAAGATTAAAGCGATAGGGGGAAGAGAACTTATGGAACAGGTAAAGAAGGAAGGGTTTTAAGAAGGCTGTTCCATTAAGCTTGAAAGAAGCTCATGTTTCGTTTGTTCTTGCGTATTTCAATGGATTTTAATAATCCTTGGAGAATTAGCTTGAAACTTCTCAAATTGTTCATCAGTATTGTCTTGTTGGAGTTTAGAAAGATAAGCTTGAAAAAGTTCTGGTGGAAGGTTTATATTCATCTTTTGAGTAAGGGATAGGAAACTATCTTGAGCACTTACTTCTTGAGCTGAAAGTTCATAAAAAGCAAGCTGATCTAATTCAGTCCCATTTAAAGAATTTGTATATGCTCCTTGATAGATTTCAACCTCATTCTTCCTTACAAAATCAATAATTCTTGAAGGCTGAACTGAGTTATCTTTGAGAAAAAGATGGAATCCATAATTTCAAGTGGATTTTCGACGCAGGAGTTCACATCCGATTGCAAGATTATCTTGAGGAAGAAGCTTTTTAAGCTCCATGAGGAAGACTAAAGAAAAATCTACACAAGCTCCCATATAACAGCTTTCTCAAGAACTTATAATAGCTTCTATCGTTCTTCTCCCAAGTTCATTCTGAATTCTTTCATTAGACCATTTGACCTTTTTAAGCTTTCAAAATAACTTTGCAATATGTTCTGCAAGATCAATATCTGGAAGTGTATTTGGGGATTCTTTAGCTCAATTGATTCCTCTCATAACAAAAGGTAAGAAAATAAACTATTGCTGAATAATATAATCATCTATTAATTAAAAGTCAAGTATTTTAGCATTCCTTCTCTTATGAAACAACTTAAAAAACCAGATTTTAAGACTATTTTCTCTTACTTAGAGCAGCTTTTTCCAAATGCCGATACTGAGCTCAACTACGAAACGCCTTTTCAGCTGCTCGTTGCCGTGATTCTTTCTGCTCAAACGACCGATAAACAGGTCAATAAAGTCACAGCAACACTCTTTCAAACGATTAAACAGCCGGAAGATGTGCTGAATTTGGGGCATCCAGCTTTTGAGCAAGCGATTAAATCAATCGGACTCTACAAAGGCAAAGCAAAAAACATCTATCAGACTTCTGAACTTTTAGTTGAAAAAAAAGGAAAAATCCCAAAAACTGAAGCTGAACTCATGAAACTCCCTGGAGTCGGAGAAAAAACTGCTAAAGTCGTGCTTCATGTCCTTTATAAAAAGCCTGTAATCGCTGTAGATACGCATGTCCATAGGGTTTGCAATAGGCTTGGTATCGTAGATACTACAACTCCACTTCAGACTTCAAAACTTCTTGAAACTCTTATACCAAAAAAGTATAAACAGATTGCTCATCATTGTATTATCCTTTTTTGAAGGTATTATTGTACTGCTAGAAATCCAAAATGTGCTCAGTGTAAGCTTAATCAGATTTGTCCTTATTTTATGTCTTTAAAGCAAGAGTAGTATGCCAAAGATTTTTATGGTAGAACCTGAGTGGTGATTTGACTTTTTGCTCGCTATTGCAGAGCTCTGAGAGAAGTATCCTTGGGTTGGGAATTTTTATCCGATAGGAGCTGATATTTTTGTTTTTGTGTATCCTGTTTTTTTGACCGTGCTTTATCTCTATGGAATGGTTAAAAAAAATCGAGAATCTAAGAAAGCTGCGCTCTTTATTTTCTTTGCCTGTTTTTTTTCGGTGGTGGTCAATATCCTTTCTCAACAGTTTTTTGATAAACAGAGGCCAATCCATGAACTCGGAATTGTAGATGTGGATCAAGAAACCTTGCTTCATCATCTTTTACCATTGACTTCGTTTCCGTCTGATCATGCGGTAGTTACCTTTTCGATTGCAATGGCGACATGGCTTATTTCTCATAAATATCGCAAAATGCATCTCAAAATTTGGTCATTCTTTTTGTTTGTGTTTGCGATTCTGACGGGGATTTGCAGAATTGGGACGAAAGTGCATTGGACTACTGATATTCTTGCAGGAGCAATTATGGGGCTTTTGGTACCAGTGGTTTTGGTGATTCCTTTCTTTTATAAGAGAATTGAAAGCTGAATTTTGATTCCACTTATTCGTTTTGAAGAGTGGATCTTGGGAAAGTTTAAGAAAAAGTAAACACAGCTAAAAGGGGAGGATTTAGTATGCTTCCCCTTGCTTTTTCTGGGTAAATAAATATAGTCCTCTTGTCGCCGCGATAGTTCATTCGGTAGAACGCATCCATGGTAAGGATGAAAGACGAGTTCAAATCTCGTTCGTGGCTATGCTTTTCGTAGCAATTATTCCTGTGAGCTTTTGCAGGTTTTTTTTATTGTTTGGATTCTTTTGAGATATTCTTTTTCGGAGAGGAACTCACGCTCGGTTCAGCTTTTTTCTTCGTGACCAATAGCAAGGTCAGGAATGAGGTGAAAATGCATATGGTTGACACTTTTCCCTACTTTCCCTCCTACAAATCCGTCTCTCAGGAGTAAATTGATATCGGAATGCGTCTGGTGGAGAAAGTCGTCTCGTTTGCTGACGAGGGACATAAGGTCTTTTACTTCCTTGGTGGAGAGATCATTGAAAAGCACGACGTGCCTATGTGGAATAATAAGTAGATGGTCTTTGATATAAGGGGCACGAGCTAGGGTAACAAAAAAATATTGAGAGCTTTCCAAAATAAATTTTGGTGGCATGTCGCAAAAAGGACAGAGATTATATTTTAGGTTGTGGGCAACGATATCGTGGTATTTGATTTGCATGGCTGGATAATTAGCGAGAAATACGAATTAAACGAGTGTTCTATATCCTGGAAGAGAGGAACTTCTTTGCACTCTTTTTCAACAGTTTCGGTGAAGAGTCTGATAGGTCTTGTATTTTGCTGGTGGGAGAAATAGAATCTCTGGTTTTATAGTTTGTAGGTGCTTTCTTCTCAGGTTTTCATATCTTTGATTTTGTAGATGCCAGCTTCTTTTTCTCCTTGTCCGTAAATTATCACGTAAGGAATTCCTTTTTTGTCGGCATAGGCAAATTGTTTTCCGAGTTTTTCTGCTGTCGGAAAGATTTCAAAAGTCTGACTTCTTTTGGCGAAGGTGCCTGCGAGTTTTAGGATTGATTCAAAATTTTCTTCGAAATGGAGGAAAAGATAGTCAGCAACAGTCTGCTTTTTTTCCTCTTTGAGCTCAAAAATTTTTCCGAGCAGTCTACTAATCCCGATACTTCCTCCAACTCCGCAATAATCGCTTCTTTTCGGATCAATATATCCAGTCAAATTCGCATATCTTCCTCCTCCACAGATAGAACCGAGCTTTTCGTCATTTTTGAGCACGGCTTCAAAAATTGTTCCCGTATAATAGTCTAGTCCTCTGATAATTTGCAAATCAATTTTCCATTCAAACTCAAGATGCATTGCTTCCTGAATCTGTTTCAAAAGCGTAAGAACGGTTCTTAATTCTTCTACACCTTCTTTGAATCCTTCCGAATCTGCAAAGCCTAAAATCTGATCGCAGTCTTCAATCTTCTCTCACTGAAAAGAGATAAAATTCAGGATCTTTTCGCTATTTTCTGCTGAGATATTGAGGTCTTCTCTGAGTGTTGCGATAAAGTTTTCTGTTCCAATCTTCTTGTACTTATCAATAAGGGTTGAAATCTGTTGCATTTGCTCTTCATCTTGACTGACTTCTGCGAGAAATGCTTTGACGAGCTTCTTATTATTGATATGAAAAACAGCAGTATCTTGAAGATCGCAACTTTTCAAGATTTTATTTAGCGTAGAACCGAGTGTTGCGATGATCTCTGCATCATAGTAGAAATAGTCCTTTTCTCCTTTCCAAATCACATCAATATCGCATTGAAAAAACTCTCTATATCTTCCTTTTTGAGCTCTTTCTCCTCTCCAAACCGGCTGAATCTGGTACCTTTTGAAAGGAAAGGTCAGCTGATGCTCCCAATCCAGCGTATATCTCGCAAATGGCACGGTAAGATCAAAATGTAATCCGTATTCTTTCAAGTCTTGTGCTCCCTGAGCGATTCCGTAGAGTCCAAAAATCTGTTTTCAGGTTTCTTCTCCATTTTTTGAGAGGAGTACATCATTTCTCTCTACAGCAGGAGTGTGGATATGGGTATATCAAAACTGCTGATAGCTCTGCTCGATGGTTTCTTTGAGCTGATCAAAAATTAACTGCTGACTAGCACTATATTCAGGGAATCCACTAGGACGAAGTTCTGCCATATGATGAAAGAAAAAAATAAATCTGATTCTTTTGTAGCGAAATGGAGTGAGAAAGCAAGATTTTCCAGATTAAAGAAAAGAGCTGATGTAGATCTGATCATCAGCTCTTCCCTCGTATGCGAAAAAACTTAAGATAAAATTATCAGTCTATTTCTGAGCTTGAAAAATACTTTCCAATAACTAGAGTGGAAGTGTTTTATTTTTGTGGATTGAAGAGATGGAGTTTTCGTTTTTTCGTGAGGATGCTACAGTGCCATCAATGCAGGACTTTCTCTTGGCATGAGGGGTAGTGGTCTGATTTTTTGTGCTTTTTTGTTTGATTAAGTTTGGAGCGAGATTTTTGCTTGCGAGACTCGGGAAATGAGCTTCTCTCAAGAAGGATTTCTTGGTGATTTTGTCAGGATTGTCGTGGTATTCGGGGGTTTCTGTGGCACTCTTTTGTGGAAATCTCTTCCTCCCGTTTGTAAAGTGGATCCATGTGGTTTTTTCAGGTTTTTTTGTTGTTGCATTGGTTATTGAGCTGAGTGGAATCCTAAAATTTTTTCTAAAGGTCGTGATGGAAAAGGGATTTGCTCATCAGGACACGAAAACTAAGAAAAATTTGACTGAAATCCTTAATACGCTTCTTCAGATTCTGATTTGGATATTGGCGGCATTGATTTTCTTTGATACGGTGGGGGTTGCGATTACGCCGCTCCTAGCATCGCTTGGAGTATGAGGAATCGCGGTTGCTTTCGCCTCTCAGAAATTGATTGAAGATTTTTTCAGTTCGTTTTCTATTATGGGATCGAGTCCCTTTAGGATTGGTGATATTATTACGATCAAAGATACAACTGGGACGGTCAAAAAGATTGGACTGAGAAGCACTACGCTCCTTACGGTGGAGGATAAATCGATCATTATTCCCAATAGACTTTTGGTCGCAGAAATTATCGAAAACAGTGGAACAATCAAAGTCAGAAGAAAGAGATTTTCACTGACGATCACGTATGAGACGCCGCTTGAAAAGGTGCGTCAGATTCCTAAGCTTTTGGAGCAAATTATTGGGAAGCATAAGGATGTTGGATTTGAATGGGCGGTGCTGAGGGATTTGCAAGCATCGTGCTTGGAGATTTATGTTAGTTATGTGATTAATAATGATGATTGGCTAAATGCTACCCAGCTTCATAGTAAAATCCTGGAAGAAATCCTAGAAAAATTTGCGGAGGAGAAGATTGATTTTGCCTATCCAACTCAAACGCTCCGACTCAAGAAATAATTTTACTTTAAAGTCCCCTCAATGCGAGATAAATTGATCACTATTTTTTTGGAACAGAATCAAAAACTGAATCTATCTGCGATTCGTGATAGGGAAGGTGTCAGGGTGAAGCATCTTCAGGATTCTCTCAAACTTCTCGAAACCGGTCTCTTTACTCCGGGGAAGTTTGTGATTGATGTCGGGACGGGGGGCGGCTTCCCGTTGATGCCTTTAGCGATGAGCTGTCCTGAGATGTGTTTCCTCGGAATTGACTCGGTAAGGAAGAAGACGCTTGCTGTGCAAGCAATGCTTGGTGAGCTTGGCGTGCAAAATGCTGAGGTATTGCGAACTAGGATTGAGGACTACCAGGGAGAAAAGGCTGACCTTTTGACTGCGAGAGCGGTTGCCTATAGCGATAAACTTTTGCAGTGGAGCGTTCCGTTGGTGAAGAAAGGCGGAGCTTTGGTATTGATGAAACAAGTTATTCCTGAAGAAAAAAGGCTTTTGCAAGGGCTCTGTCCTCGTTTTCAGCTTAAGATTGAGCAGGAGTTGAAGTATCAGCTGTTTGAGGGGGATATTGAGAGAGTGATCTATGTGCTGAGGAAAAAGTAACATATCTTCAGGTTTTTAAATCTTCTTCCTCTCGGGTGGGAACTCTTTCGGAGGAGATGAAACGTTTTTGATTACGTGGTTCGTGAAGGGGAGGGTGGATGCTCGTGGGGAATCAGTCTTCACCTTTTTTCTTTTATTAAAAAAAGGAGTGCCCCTTTTCGTAGAACGCTCCTTTCTTTCCTGTTGAGATTAGTTTGCTAGGGGAAGATATTCGACTTCCATATAAGAAGGCTGGAGTTTATCTTCTGTAGCGAGACTCATTCTTAGATGTGTTTTTGCACCACCTGCGAGCGTGATAATATCACAATTTCCACTCTTTTCTTTCATTTCATTGGTGATCACAGCACTGTCGTAGAGTTGGATACAAAAATCTCCTTGGTATTTTTTCCTCAATGTATTAGAATAATAGTCTTGAGCAAGGTAATGATAGTCGAGTTTATCGTATGCGGATTGTTTTTCTGGATAAGGATTCATGAGCTCCATATTCTCTAATCGGAATCTTGCATTTTTGAAAGTGAGTCTGATTTCTCTGTCTCTTTGTGGCTCGATTTCTAAATTTAGGATCTCTGCTTTCTCTGGATTGGCTGCTATCCTCATACCAGAGGAGATTGGCACTGTTTTTACCTCCTCTTGACGATAGCCTCCGAAAGGTTCAGGCTTCCGACTGTTTTCTTTTTCTCTTTCTTGTTTATTAAGCTCCATCAACTTCATATAAAATGTGTCTCTAGCTCCAAAAATTACGGTTTCTCCTCTTTTAATATCTTCTTTGATTCCGACTGCGTGCATGAGCCCGATGATGGCGAAGCAAGCTCCTGTAATCATCGTGATACTCAAGAAGAATTTTCTCAATCCTCTGATTATTCTGGATTTTTTCTTGAGGAATGCGGTGATGGCGTAGACTAAGATTGTGAATCCGACTGCTCGAGCGAAGACTTCCAAGAGCGTGTTGATAACAAGTTCTGCTGGTGAATAGAGTCCCATGATAATAGTATACAAGATAAAAATCTGATCCCTTTGTGCGAGGGATGAATACTTCTAAGCTAATTTTACCCTTTAGCTGTGGGAAAGTCAACTTTTCTCGGAGTATTTTGCTTTTTAAAAGGGGCTTTTATATGAGCGAGGAGATGGAAGTGGCTTGTTTATTGTGTTTGTCAGATGACAAATTGCCCGCAGAGACGTTTTGAGCCGTTTGTCAGATGACAAATCGTTCCCAGAGACGTTTTAAGCCATTTGTCAGATGACAAATTGCCCGCAGAGACGTTTTAAGCCGTTTGTCAGATGACAAATCGTTCCCAGATGCGTTCTGGGACGAGAGGTCCCTCCGCTTCAAGAAAAATAACGATTTGAGAGCATCTCCATTCTGGAGTTTCGCTTGAAAGGGTATTTTTATGGATTGCCACGCTGACGCTCGCAATGACGGGTGAAAGTCCCCTTCGGCTTCTCCTTGTCCCCTCCTTGAATTTCACAAACTCCCTCAATCCTTCGGACAGCTCCCTATTTAGAGGAGCGAAATCGGTGAGGATTCTCTTTGGTCGTTTAGCAGGGGGAAAAATAATAGATTGCCACGCTGATGCTCGCAATGACGGGTGAAAGTCCCCTTTGGCTTCGCCTTGTCCCCTCCTTGAATTTCACAAACTCCCTCAATCCTTCGGGTAGCTCCCCTATTAGAGGAGTGAAATCGTCGAGGATTCTCTTCTGTCGCCTAGCAGGAGGAAAAATAATAGATTGCCACGCTGACGCTCGCAATGACGGGTGAAAGTCCCCTTCGGCTTCGCCTTGTCCCCTCCCTGAATTTCACAAACTCCCTCAATTCTTCGGACAGCCCCCTATTAGAGGAGCGAAATCGGTGAGGATTCTCTTCGGTCGCTTAGCAGGGGGAAAAATAATGGATTGCCACGCTGACGCTCGCAATGATAGCCTTGGTACATGAGTTTACAAAAGTTTTTTTACCAAAATTGTTTCTGGAGGCAATTTGTCAAAAGTTTTTCCCCGTGTTCGAGGATAAAGGATTGCATCTGTGGAGCAAAACGCTATGGTTATTCTGTTTATTTCTGATTTTACGTGATGAAAACCAAGCTCCTTGACGAACTTCAAAGTAACTGAATCCTAAATTTTTCTCTCTTCCAAACTCTAGAAGCTTTTGCAATCCTTCCTGATCTGCTTTGAGAACTTTTGGAACAGGAAAAAGCTGATTTTCAGACCTTAATCCAAAAAAACAATGAAAGTCTGACTTTTGAGGATTTTGTGCCAGAATCTCAGCTTGCTTACCTCTGGAAAATCATCAATCATCTTGATGCGGTAGAAGCAAGCGAAGAAATCAGAGCGATTATTGCAGATTTTAGACCAAAGTACGAGGATTTTGTGAATGAAGTGGCTTATTCTCAGCTTTATTATCAGAAGCTGCTCCGAGCAAGCGAACATTTTGAAAAGAATCCTCATCAGCAGAGGTGGTTTGATTTAACTTTGAAAGCTTTTGAACAAAGGGGAATCGCATTGCCAGCAGAGGAGCAAGCACAGCTCAAAAATCTCAATAAACAGCTTTCGGAGCTGGGAGAGAAGTTTCAGCATAATGTCGTAGATGAACAGTCAGCTTTTCTGCTTTATTTTGATAATGAGGAGGATTTTCAGGAGATGCCGGAGGAACTCTTGGCGAAAATGAAAGCACTTGCTGGGGAGAAATGAGGGCTTGCAGTCAATGCCGATCCGAATTTGCTTGTTGCGGTAAGTAAATATTGCAGTAATCCAGAAATTAGAAAGCAGGTGGCTTTTCAGAGAGAGCAATGGGCGAGTGAGGGAAGATTTGACAATAGACCTTTGGCTTTGGAGCTTCTAAAACTCAATCAAGAGAAAGCAAAGATTCTTGGCTATGCGAATGCAGGAGAAATGTTCCTTTGGGCTACAATGGCTCAAGATCCGAATCAGGTTTTTGATTTTATCAAGCCGATCTCTGCGAAGGCGATGCAAAAAGCGAAATCTGAAGTGCAGACTTTGCAAGAGTATTTTCAGCTTCCAGATTTGCCAAGCTACGATCTCGCCTACTATACGAGGAAGTATAAGGAAGAGCAATATGCCCTCAATGATGAGGAACTCAAGCAGTATTTTGAGTTTGAGTCGGTTTTGAAGTGGCTCCATAGCTTTGTATTCAAGTTTTTAGGTGTGGAACTTCGCGCTAGAGCAGAATCAGCGTTTTGATCTCTTGGGCATGAAAGGCGGTACGAAGTCTATCATCAAGGAGAGTTGGTGGCGTATTATATGCTAGATGCTTTTTATCGTAAGGGGAAAAGACCCTGAGCTTGGGCAGATTACTTGAGAGAGAAGGGGAATGGGCAGTTGCCGATCGTCGTAAATGTTTGCAATTTTCAAAAAGCTGAATCCTGACCAAGTTTGCTCTATCTGAGAGATGTTGAGACGCTATTTCATGAGTTTGGACACGCTTTGCATGCGATGTTGGCGGGTTCTCCCTATGCGGAGCTTTCTGGATTTAATGTAGAATGGGATTTTGTGGAGCTTCCAAGTCAATTGATGGAAAATTGGGTGAGCGATCCTGAAGCCTTGAAAAGTTTAGCAAAGCACTATCAAACCTGACTCCCAATACCAGAAAAACTTGTTCAAACTCTCCAAGATCTCAAAACCTTTATGATGGGAAACTGGGTAAGCAGGCAAAATGAGTTAGCTTTGCTGGATATGACGCTCTACACTTCCGATATCCCAAACTCGGTAGAAGAATTGGATCAAAAGACTCTTGGAATCGTGAATCAATATTCCATTTTTCCAAGAGGGGAGGAATACAAGATGTATTGTGCGTTTCTGCATATTTTCGGAGGTGGCTACGAAGCGAAGTATTATTCCTATATGCGAGCAGAGCTTTTGGAGGCTGATGTATTTCAAACCATCAAAAAAACCTGAATTTTCAATCCAGAAATTTGATCTGCCTACAAAGAGAAAATTTTAATTCCATGAACTCGTAAACCAGCTGCAGAATTATTCTTTGATTTTATGCAGAGAGGACCAAGTGATGAGGCTTTGATGAAGAAGTACGGTCTTTTAGCTTAATTGGCTGAAAAAGAGAGAAAATAATTTTTCCCTTGGAAGGTTTTATTTCGATGAATAGTATGAAAATTGCGTTTGTGAATTGCTGGACGATGCCAGGTGGTGTGCTCAATGTCCTTCAAGATTTGATGATTAAACAGCTGAGAAAGCATGGGAAGGAGGTGGAAGTCAGGCTTTTTACGCTTATTTCGGATCGTGAAAGTCTAGAACTTCCCTTGCCATGAGGAAATGGAGCGATGGTAACGATTCCGATTGTGCAAACCTTACCAAAATGGATCAATCAGATTTTTCTTTTTTTTACTAAGAGGAAAGTGAAGATTTTGTCTGGTGTTTTTGATTATCGTAATTTGATGGTGTTTTATCCTCGGCTTATGAAGCTCCTTTCTTTCAAGATCAAACAACGAAAGCCTGATGAGATTCAGATTTCTTCTTTTGCTATTGCGAAAAATATTACACCGATTCCGTGAGTGCCGACGCATCTGTATCTGCATTCTCCGATGCAATATATCTGGAGCCACTATGATGAATACGATGCAAGGCTGACTTGATGGAAAAATAGGCTCTTTAATTTTATCGTTCCAAGGCTTAGGAAATGGGATTTAAAGTTTCATCATTTTGATTCGGTGGTTTGCAATAGTCATTATACTCAGGAACTTGCAGAGGAGCTTTATGGCCTCAAAAGTACGGTTTCCTATCCAAAGATCGCAGATCAGTATTGGTTTGCTTGAATCTCTCCAAAGCCGATGCCTTATTTTGTGTATGTAGGGAGGCTGGTCAATTTTGTGAGGGAAACGGGCTTGATTATTCAGCTTTTTAATCGTTTGAAATTGCCTTTGGTGGTGATTGGTTCTGGACCTGATGAGGAGGAACTCAAAGCTTTGGCGGGTGAGACGGTTATTTTCACTTGATGGAATCCTGAAGGAATGACGGAGATTGTTAGAGATGCTGCTGGACTCATCAACTTGACGAAAGAGAGTTTCTGAATTTGAACGGTAGAGGCGCTTTTGATGGGAGTTCCAGTATTTGGTTTCGCTGAAGGTGCGAGTGCAGAATTGGTGGATGAGGATTGTGGAGTTTTGGTGAAGAGTAAGGATTTAGAAGATTTGGTGTTAGATTTTGAACTCTTTCGTAGTAAAAGCCGAGATAGGAAGCTTATTTCTGAGAGGATTAGAGAAAAGCTGACTTCCTAGTTTGTTTTATGGCTTATTTGGACTGAAGAAGATTTGCTTTTGATGGAAAGATACGTATAGTCTTGTGGTATTTTATATTCATTGAGGTATAATATGTGAAACTCTTGTAAAGGATTATTTTTGTTGGTTTTGGGTTTTGTTTTTTTCGGTGGGAGGTGGAGTTGTCCTGCTTTGGCCGCGGTAAGTGTTCCTAGTCAGACTTTTGATCTGTGAACGAATGTCGATGGAGTTTGGGATCAAGGGACTCAGACATTAACTATTCAGCCTCGTTGAGCAGGGACAGGGCAGATTGATAGTGCGAAGTGGTTTCCGATGGTGACTGCACTTCAGCTTACGGGCGAGGAAATTACTAAGGTAGAATTTAAGAATAATGTCTGGTTCCCGAACGTTGCAAATGGCTTATTTCAAAATTTTAAGAGTCAGATTATTTTCCCATTAACTCAATATACGAGTAATGTTTGGTCGATGCTGAATATGTTTAATGGGGCAGTAAAATTTAATTCCGACATTTCAAACTGGGATGTGAGTAATGTTACCGATATGTATTGAATGTTTGCTGGAGCAAAGCTCTTTAACTCCGATATTTCTTTGTGGAATCCGCGTAAGGCAGCTCGTATGATTGCGATGTTTCAATGAGCAACGAGTTTTAATCAGCCGATAGGGAATTGGGATACAAGTAGTGCAACTGATATGTCTTTTATGTTCAAATGAGCAACAAGTTTTAATCAGCCTATAGGAAACTGGAATGTCGGCAAAGTAAGGAATATGACTTCTATGTTTAATTGAGCTATAAATTTCAATCAACCAATATGAAACTGGGATACGAGTAAAGTAGAAGATATGAGGCAAATGTTTGCTTTGACGAAGAATTTTAATCAGGATCTTTCTGGTTGGAATGTCTGAAAAGTTAAAATTATGGTGGGGATGTTTTGAGCGGCAGAGAAATTTAATGGAGATATTTCTACTTGGGATACGAGTAGTGTGGAGAATTTCGGTTTTATGTTTGAAAAAGCTAAGAAGTTCAATCAGCCAATAGGAAATTGGAATACGAGTAAAGCGACCTATATGGCGTATATGTTTTGGTGAGCAACGGATTTTAATCAATCTCTCAGTGGATGGGATGTGAGTAAAGTTACTAATATGGATTCTATGTTTAATGCAGCGACAAGCTTTAATCAAAATCTTTCTACTCGAGATGTAAGTAATGTTGTAGACATGGGAGGAATGTTTGCCAATGCCTCTTCCTTCAATGGAGATATTTCCAATTGGAATACGAGTAAGGCGCAGCGTATGTGGTGAATGTTTGAATATGCTCGTAGCTTTACTGGAGATGTTTCTGCTTGGGATTTTACTAAGGTTTATAATCCCTGACTGCCAGCTCAGGCTGGTTTCTCTATTAGGGCTCCTGGTAAGAATCGATATTGGTATCCGAGTGAAAATTATAAGAAGATTTTGCAGGCTATTAAAGTTGCTTATACTGCGGGGACACTTAATTTCTCTAAGTTTAGAATATTGTGAGTTGAATCTACCTATTGTGATTTCTATGGGCTTAGAGATGAACTGATTTCTGCAGGATTGCAAATAGTCAGTGATAAATTTGACTGTGAGCCGAAAATCACGGCTACTGCACCTACCTTACAGAGTAGTGGAGAAATTACGGATACTAGGATTACTTTCAGGAGTGAGTTTCCTTTGACACAAACTGATCTAGATAATGGAGCACTTTCTGTAGATCCTGTAGGAACAACCGTTCAATACTCGAATTTTAACTGTAGTTTAGAACCCTTAGATGCTACTAAGGTAAACTGTAGCGTTAAGATTACTTCTACACAAGAACAAAGCGATAAAAATCTGAAACTTAAATTCTATAAGAATCTCTGAAATAATAGAATCATTCAAACTTCTGCACTTATAACTGGCTATCTTATTGATACACAAGCACCAAAACCAGCTCAACTCGATATTAATACAAGCTCAGGTATTCATACACCAACCGTGACTCTAAGAAGCTTTGAAGATGTCTGAGCGGCAGGAACTGGAACTTGTAAACTTACTTATACTGGTGCTTGAGGAATCCCTCAAACGATCACTCCACTTTTGCTTGGAGCATCACACGTTTTGAACTTCAACCCAACAGAAACTGTTCATACGGTGAAAGTAACCTGTTCAGATAAGGTAGGGAATGTTTCAGAGAATGAGATTAAGTTTCCTCCAATTATCGAGTTTAGTCCGAGTAATGTTACAGTTAGTAAAGACGTAATGAGCTGAACTTTTACGGTTTATTCTCCAAGTAGTTTTAAGATTAAGCATATCAACGTTGCAAATCCAGCAGATACTGGAGTAACAAAGATCATTTGTAATGGGCAGGATTTGTGATTAGGAGGAGCGGTTGATTTTGATAATGGTCCAACTAATAAAGTGCAATGTAGTTTTCAATGAATCAATACGAGTGGAATCCTAAAGATTATCGCAAAGGATGCAAATGGTGCTGAAGGTCAAAATAGTGCGTTCTTTATTCATGATACGAATCTTCCTTCTCTTTTGATTACACCACTTGGTATGTTGACGGGAGGTGATCTTGCCTTTACGGTAATCGCTACGGATGATCAAGGAATCGCTGCTACAGGAGTTCAAATTACTTCTCCAGGTGCAGCAATGAATATCCAAAACCTTGCTTGTGTTCAGGTGAATCCTACGAAGGTGCAATGTGATTTTACGGTAAGTGATCCTGTGATTAGCTGAAGTATTAAGGTTGATATCAAGGACCTTGCAGGGAATCAAAACTCAAAGACTGAATCAAATTATACGATTGATAAGACCCATCCTACGATTTCCTCTCTTAGTTTTACGGCTTCTAATCATGGAAGGAAATGGAAGATTTCATTCCAGACGCAAGACCAATGAGGTGCAGGACTCTGGAAATCTACAGAAGCAACAGATCCTGATTATGATGCCTATGCAATTACCTATGGAGTAGGTTTGGATGTGAATTGTAGTGGATATATGAGTTTAGATGGGGTGGCTCCTGGAGCTACAGAACCCTTTACCCTCAACTTTGAAATTGATGATGCAAATCAGAATGGGAAGTATCTTTGTGTAGTAGTAAAAGATAAAGTCTGAAATACTCATACGGGAGTCCTTGCATCAACTATCAACTTAAACCTTGCTCCTACCCTTACCGATAAAACGCTTACGCTTGATGAAAATACTCCGATTCCTACGCAAATCCTTACTTTCGATGGGCAAGACCAAAATACTGGAGATACATTGAGCTACCAGATTATCGGTGGAAATACCGGCAACGCCTTTTCAATCGTTGCAGATAAACTGCAGGTAAATGGAGTACTTGATTATGAGACCTTGAGTGGCTATCTCTTAACAATTCAACTCAAAGATCAGCTCGGATTAACAGGAGAAGCGAAGGTTACGATTACTTTGAATGATGTGGATGAGATTCCGCCAGTGATTAGTCCTCTTACTGGAGTGAGCTATCAACGTTCTACGCCTATTGTGCCTATTCCATTGGTAGCAACTGATAATAGTGGATCGGTAACGCTTCAGGTAAACGGATTACCAGCAGGATTGACCTTTGATATGACGGGTTCTCAAATTATTGGAACGACTCCAGCTACTCCAGGAAGCTATCCTATCCAAGTGGTTGCGATTGATCAGGCAGGAAACCAGACTACCGTTCAGTTTACACTTACTGTGATAGCTCCTCCAGCTCCTAGTAGTAGTTCAAGTTCCTCTTACCGTTCTGGTGGAGCAGGAACAAGCTTACCAAAAGATAATTGTCCTAACGGTGATTTCTCATCAAGCTATTATGATGGTAGCTGTGGAAATGAGTGAAAGCCTGTAGAAACAAAAGAGGTAGAAAAGAGTCTGTTTAATCCAACAATTAAGACGACGTGTTTTAGTCCGCTTGATCAGAAAACGATTGATCAGGGAAATGCTATGACAGAACTCTTGAGACAAGCTCATCAGATGCTCTATAGTTATCAGCTTACAAGATGGCAAGGCACCAAAGACTTTGCTCCAGAGAGAAGCTTAACCAGACAAGAAGCAGCAAGATTTATGACTGAATTTGCTACAAATGTGCTTTGTAGGAAACCAAGTAGAAACTATGCACATCAGTTCATAGACTTAGGAGATGCTGATCCAACGCTTCTACCTTATATCTATAAATCGTATGATTATCTTATCTTTAATGGGGATGGGAATCCAAACGGAGATAAAGCACAAACGACGTTTAGACCTTATGATCTGATAACGGTAGATGAACTCTCTGCACTTCTCACCAGACTCGTGAAGAATCAAACCATGGAAGAGCCAGCAGAAGATCGAGCAAGAAATTATAGGAATTATATTAGTTCTATCGCCTCAAACTCAGCTTTAAAGAATGATATTAGATGAACGGTTGCTGAGGTATTGTATGATTTGTATAGAAATAATAGCTATGACCTCAAAGAAGTAGGCTATGTGATCAAAGACTAAATGAAATGTATAAAAGAAAAGGGAAATGAAGTCAGATTTTTTTCCCTTTTTTTGTTTTGTGATTATTCGGTTTTTAAGATTGATTTTCCCCCTCTACTTATTCTTTGTGATTAGGTTTTCCTTGTTTGGGGCATTGGAGGCGGAAAATTAAGTGCTTTACTAGATTCTTGGTGGAGAAAGGGGCGAATTTTTCTGCTTTTCGAGCTTATTTTGCGGTAGTGTTATTCTTCTGAGGAAGTCTCGGTAGCAGATTCTTGTTCTTCTCATGGAGTTCCTAGATCTGGGAGGTCTTCCAAATCTGCTCCCATACCAAGTCTGGTAGAGATTTCTGCATCAACGAATTTCCTTGCTCTTCCGTATTTTTTTGCAGAGTATTCCTGGAGGAGAGGAACGATTTTCTTGTTTTGATAATCTTTTGTATAGATTGAATTCATACTGAAAACCTTACTTGTTGCATTGTCAATATTCAGTTTTATGTATGATTTATAATTGGCGATTCCTACGATATCTTGAGGTCAGAGGAGTGGTGAGTACTCTTTTTCCATAAATTCTGCATCGGGAGCACCGATTTTGAAAGACATCATCGTTCCCACGTTACCGAATACTGCGGCTTTTACATCAGATTTTCCGCCTCATGAGTCTCAGAGTCCTTTCCCTGCTTCGAGCTGAGCGATATACTGGTGCGCCATGATCAGTCCAAGCCTATATTTTCTGGCTTCGGAAAGGATATCAGCAAAAGTTCCCGATACAAAGTTTTGGAATTCATCCACATAAAGATAAAAGTCTTTTCTGTCTTTAGGATCGAGTTTGGCTCTTCCCATTGCTGAGTTATAAATTTTGGAAACCAAAATCATTCAGAGCAACTGTGCGTTCAATTCTCAGATTTTACCTTTTGAGAGGTTAACGAGGAGAATCTTTTGATTATTCATCACGTCATCAAAGTTGAAGGCGGATTTCGTTTGTCCGATGATATTTCTGATCAATCTATTGGTATTGAAAGATACGAATTTTGAAGAGAAATATGGGATAATTTCAGCCTTTTCTCTGTCTCCCATGGCATTATAGGTCCTTTCTCGTCGATTTTTTACGACCGCATTGGTGACCTTTGCAACTTTTACTTCTCTATAGGTTTCATCAGTAAAAAGTCTGACGACATCGAGCAGGGTTGGTCTGTCTTCAAAGTCTTCAAGAATTGTGAGGCTTCCGTATTTAAAATATTCCTGAATTCTTGGTCCGAAAATTTCTGGTCCAAACATTTTTAGAAAGATTTCTGTTGCATCGTTTACGGTTCTATCTGCTTCATCAAGAGTATTGATTTCGTAGAGATTAAGCCCCATTGGACGGTCTTCGTTTCCTGCATCGAAATATACTACGTCTTTAGCTCTTTCTTTTGGAACGAAACTTAGCACGTCTTCTGCAAGGTCACCGTGCGGATCGATAAGGCAGATTCCGTCTCCGTTCCAGAGGTCTTGTCTTGCCATGGTTTGGAGGAAGACTGATTTTCAGGTACCAGATTTTCCAATCATATAGTGGTGTCTGGTTCTATCTTCTCTCTTCATATACACAGGAGTAAGGATATTTCTGTAGATATTTGTTCCAAGAAGAACAGCGTTTTTGTAGAGTTTGTAGCCTGTGATGGTTTTGCCAGGGACGATCTCCTTGACGAATTTTTGTCCGTCTTGTTCGAGGATTGGATTATTATTGATCGTGGATTTACCATCCTTTTCCTCAATAGAAGTTCACTTGTGAGCAAGCTGATAATCTGCGAGAGGAGTCAGCTTTTCTTCTTCTAGTGTCCTTGATGCTACGGCTCGGTGGTTTTTGTATTCTTTCAGAATCTCGGAGAGGTTTCCATTTTTGTACTTTTCTGCGAGGATACCTGACATTATCTTGCCATTCCATTCATCATCCTTGAATTGTGGGAGATTTGCTGGTGAAGGAAGGACCTTATATTGCATCCACTCGATGATAGGAGAGCGGTTGTAAATACTATCTGCAAAATGGAAGAGTGATGTGAGTTCATTGGTCGAGAAAAAAGAGTATTTGTAGAAAAAGCCTGTAAGGTAAAAATTTACTGCAATTTTCCACAGGTAAAGGAAGATACCAGAGAAGATATCGTGCTTGGTGTTATTGTCTTCAAGACCGTTGCTGTATTCGTCGTTGTAAACATTGTATGCGGATTCCATAGCTTTCAGGTTTTGTCTGGCTTGTCCTTTTACATCTGAGGAACTAATCACAATGATTCCAGCTCTGTAGGTTGGATTTCCTGCCTCTTCACCAATAGCATTCATCGAGTCTTCTTTTGCTCTGACCATTCTTACCATTCCTACACTTTCCTCTTTTTTATTGGAGAGGAGTTTTTCACTTGGTCATTGGATTAGAAAACTGATGAGTTTGAAGGGGTTGAGTAGGTTTTTTCGGTGTCGCCATTTCGTCGAATAAAGATCAAGCCCTTTATAAAGTCTTTCTGCTGCTTTCTGTCTCTTGGCATTGAAGGTTGAGCTTTCAGGTTTGATGACGAGCATCGCAGTTATTGTGTCGTAGCTAGAGATTTTTCCCATCGTATCAATCAGGTTATTGATTGGATCATCCGGCATTTGTTTAAAAGTTCTGATTGTATAGACGGGGTCTCTTTTAGGTTCTAATGGCATAATATCATGGTATTTCTTTTGGAAAGGCTGCGGTTTGCCGACTTTCTCGAGAGAGCAGTTTGGAAATTGTGCAGAAATCGCACTTTCTATAATACTGATGTATTCGGGATAGCTTCCAACGATAAAACTTAATTGACCATTTTCGTATTGGTAGATGAAAACGATTTTTTGCTTTCAGAAGAATTTGTACATGATTTTTTCCCGAGTACTGAGTTCTGAAATTTTGTGAAGTCCATTGTATAACTGTGACATCCTTCAGACTTTTTCTTTCATATCTTTAGCGATTTCTTTTTCTTGTTCACGATCAGTTTTCCCATCTGATTTTGGCAAAAGTACCTTGATGAAAGTCATCCTTCTGGTATTGTAGAAAGAATAGATGTATTTGAGCGTATTTTTGATGATACCGATGATGACGCCAATACAGAGGAGTGCGATCAATCCATAGCAGAGGTAGATTCCAATTTTTTCGATAACTTCTCGTCCTTTTTGTAGTTCTTGTGTGGCGGTTGATTGTGTAGTTCCAGTTTGTATCCTTGTTTGTGCTGCTGTTTGCATTGTGGTAGTTTTACCTCAATTTGTTCCTCAGGTTTCTTGAGCTGATGAAGGTATGGTGGCTTTTGCCTCGTAGGTCTCGGTATAGTTGGTTTCGATATTTTTTAGAAAGGTTCAAAGCTGGTTGTAGCCTCAGATTTCTTTGAGACATTTATTCTTTTCGCTTCACCAAGCTTGGTTTGTTAGTTTTGAAAGGTTATTTTTTTGACTAAAATCTTTTCTGAATGTTGTGATGAGCTTCTCTTGGCATGGAGTGCTATTTGAATTTGTATTTGGCGCATAATGTTCTTTTTCTTCAAACGAATTGAGTACTGATCTGATTTGAGGAAAACCTCCGATTGTTGTCAAACATTCAGATTTCTTTTCTCTCCAAGCTTGATTTGTTAGCTTTGAAAGGTTATCTTTTTGTTTTGTGTCTTTGAGGTATTGTGTTCTCAAGAGTTGCATGCATTCACTTTGTTGCATTCTCATTCTGATGAGTAAAAACCATACTAAGTATAGCTATTTTTTCTCAAAAAAGCAAAAAATCAGGCAAATTTCTTTACGCTTTCTTTTCTTTTTTCTTGTTTTTGTTTCTTGCAATTGTGAGCTGATTTCTTATATTTCAATCAGTGTTTTATCTTTTCTTTAATGCTTGTGATTGATCGTATAAAAAGTGAAGTTTCTCAATATGTTGCTCTTCAGAGTAAATCTGGGGTGTTTTTGTCAGCGTTTGGTAAAGATGGGAAACTTTTACTTTCTCATGGAGTCGTTAGAACCAATAGAACCTTAGAAATGATTTTGCAGAGTTTTTATACTGCCTTTTTGCAGAAGATAGAAGCTCAAGTAGCAACTGTGATCCTAGATATTGTTCAAGATGTGAGATTGCAGAATGATCCGAATGTTTTAGTGAAGCTTCCTCTTGATCAGTATTGACTTTTTCTAGTGCAAGATGAGTGATTGAAAAGCTGAGTTCTTCTGCCAAATACTAAGGGGATAGACTCGCCTCAGCAGGCTTTTAGTGCGATTAAGCAGAAATATAATCTTGGGAGTCAGGTTTCAGTCTATATTTTTACGACCAAGAAGGTTGAGATTTCTTTTTAGTTGCAAAAAAGTGGGATTGTGTTTTAGAAAAAACTAGGTTAAGTGTTCCTAACCTAGTTCTAATGGTGCTTTCAGAGGAGACCCTGAAATTTTGTTCGCCAATACTTATTTGCGGCTATCCTTTTCTCGTACTCAGTAATCTTACGCGTAAAGAAACTCGTGTCGCACTCTCTGTTTGCTCTTTTGAGGCTTGCTTGTTTCTCTTGCATCTCTTCTTTTAGTTGTCGCCGAAGTCCTGAATCTTCATCCAAGAGATTGATGATTCCTTTGATCGACTGATTGAGCTGTTTTTTTTCTACCCAGAGATATATCGGACCTAAAATTCCGATTAGACTAGGGAGGACGAGCTCTAGGTAGCTTGGTTTGAGATTCATAAACATGGGAACGATTACAATCCCAAACATAATAGAGATTGTGAAACCTGCGATTTTGAGCTTATGCTTCGTTTCTTTCAGCTCTTTAAATAGCTCTTTATTTGAATTTTTTTCCATAGTGAAATTTTTTTTTATTACCCTTGGTATGATACTTAATTTATTATGAAAGTCAATAATAAAGTGCATCTTGCATTTCTTTTGGCTTTTGTTATATTTCAGTTAGTTTTGTTTTTTGATGACTCGTTTTATTTTCTAATGTAAGTATGATGAAAAGATTGGGAATGTGAGTTCTTTTGCTTGGTATAAGCTTTATTCTTGCTGCTTGTTTAGATAAGAGTAAAAATCAGGCTTTATCTTATGAATTTTCTCTGCTTCAAGATTTTGCTCAAACGAGTAAGTTACCGATGCCACAAATTGAGGAAGCTTCCTTTGAATGGCAAGAAGGAGAGGAAAAACTGACTGTTCAATGACGGGGATTCTCGCTTTCATGATTACAAGAAAATCCTGATGAAGCAGTAGAAACTTTCTTTCATACTTGGAAGAAAAATGAAGAAAATAGTCTAGGGCGAGGATCAGTCAATGTTATGGGATTTGAAAAAGATGGGACGATTTGTCTTAGTGAAAGTCATATTCATGCCTCGCTCCAGCAAATCAATGATGATGCTCATTTCCCACAGCATGTGCCTTATAGCCTTCGTCTCACTTGTGGACAGAAAAGAGATCTCCAGATAACTACTAAACAAAAAGAAAAAAAATCTGAAAACACGACAAATCTTTGCCTTGCAGAGATTGAAAAGCAGATTTTTGCTTCACCTCAATTCATAGGAGTTAAACAAGACTATGATAAGGCTCTGGCTGCTCCGAATCAGGGTGATACCGAGCAGTTTTTGGGCTATTATCTAGATTTGGAAAGTATAGATGATGAGAGTTATAAGCTTGCTTTAGCTGAAAATTATCCGAGCAGAGTGCTGAAATATCATCGTTTTCAGGTAGATAAAAAGACAGGAGAAATCAGACTTTATGATGTTGCTTCAGATACCTATGATGAGATTATTCCTCACGATGAAAGGTATCCAGAGTTCTTTGCTCAGCACTGTAAAAATTTAGATGATAATAACTAATCTTTCTCTTTAAGGTTTACTTTCTTTTGAAAAAAATGAAAAAAATCTGATTTTTGATCCTTATTGTTTTCTCTACTCTAGTTCTTCTTTGATGCGAGAAAGGTTTAGAGTCTCATCAAACTTCCTCTGATTCTACAAACAGCGGAGCAATGATGATGAATCAAAACATCGTTCCTTGATGATACCTTTATAGAAACTTAGACTTAAGCAAAGCAGCTGACAAAGAGGAGTATGAGCAACTTCAAAAAGATAGAAATCTGATTCTTGAATATCAAACCAAGACTTCTTCTGCGGTAAATAAGTTCATAGACACGATTCATCAAGACCTTTTAGATGCCTGCAAAGAATACGGAACCGGTTATGAAACAAATAGAGGGACTTATTATGAAAAAGATGTCTGTGTAGATCGCTATATCCGTAATTTATATCAAAAATTAAAGACTGAATTAAATAATCCACAAAAAAACTATGCTTGAAAAGATCCAGTCTTGCTCTCGTATTATCAGGATTTAGGGTGAGATCATCCTTTTGTTCGTTATTTTGTGGATTTGTATACGTATCTTCTCAAAAGATGACGAGATCTTGGAGAGATACCAGAATATCCACTGATATGACCTCTTACAAAAAATCCAGATTGATGATATCGAAATGATGAAGAAGCTGGAGGCGATTTAGCTATGAAGAATTGAAGCTGATTTTATACCTACTGAGCATGAAACATCACAAAAAATCAATTTTGATCAATGATATCAAGTATTGGCTCATGAATAGTACTCAATGGTCATATGCTGAAACGAATTGAAAAAGAATACTGATACCATTTTCCATACTGAAGTCTGGAAATTATATATGGCACTTCTCCAATGATTTGACCCTTTGTTCTCCATCATAAGACAAAATATGGGGATTATATCTATCAAATTATAGGATCCGGAGCATGATCATGAGAATTTATACTGCTGGTGCGAGTTTTTGTTGATTGATTATGGATTCCTCTTGGACAATGTGGGTATAGTTGATATGTTTTGTTCCCAAATTATCCATTGCTCTTTGGAGAATGTAAGGAACGGCAAAATAGTGATCCTCTTACGATTTTCACACAGGAGTATAATCTGTATGAACATAATAGGATACCAGATCTAGTTGAAAACTTTACTTTCTTCTTGAGGGCTTTAGAGTCTACAAGGGTATTGAGTAAATAAGTTTTTTAGCTTTTAATAGCATATTCCCATGAAGAAAATCTGATTTTTATTGCTCTTTTCCTTTCTTTTGAGCGGTTGTTTCTTCATCAAGCCAAGCGATCCGATCCCAGAAAATCAAGATCCGAAATGATCCCTCAATTCAGGACAAGCAATTTGCGATGCCGAAGATTCCTGTAATGATGAGCAAACCTCGGCAAAATTGAGTCAGAATTTAGCTGATTTGAAAATTGGAGATGCTATCGGAGGACTGACTTTGCAATCAAAGGAAATCACTCAAGACCATGCGGCTTTCCTTCTTACTGGAGCAACTAAGGTAACCTGAACGATTAAAATGTATAAAGATGAGGAAACTGATGAACTTTTGGTCGATTTCACTCCAGAGCAGACTTTGGAGACTATCCATATTTCTGATTGAAAATTGAGCAACTATAGCATTAAACTTTTTGAATGAAACATTGGATTTGCTAGTTTTGATGCACAGACAAGACAGGCGCTTTTGGCAGGAGAAGAGCTTCAAGTGGAGCTCAGTATCACTTCATTTTTTCATGTAGGATGGTTTGAGTCTGAGTATTATTCAACTCTTGAGATTAGCGGATATAAGGTTATTCTAGATTAAGGAGATCAATAAGAAGGAAAATATGTTTGCTGTTTAGATGTATTCCTTTCCATCATGATTATAATTTTATTTGATGATAACATGATATGCAAAAATTCTGTATTACATTTGCTTGATGTGTATGAAGCTCCAAAACACCTATTTCAAACTATATAAGCACTAAGTTAAATTTGCCGATATTTAATAATGATGCAATCCTTTCTGAAGTGATTGAAAATAGAGGATTTTGGGATATAGAGGAACATAAAAAACTCAGAAATAATAGACTAAAAGAAGTGTTAGATTCATGAATTTCATTTATTGCGGATGTAAGTATTGATAGAGAATGGGAGAGTTTTAGAGAAACCTTATTATCCTATGGTTATACACGGTTTATCATTAGCCTAGACTTGAGCAAAGGACTTTTGATGAAACTCTATAAAGCTAAAGGGTATGATGAGTCATTAAAAAGGCTAGATACCCTTATGCATGATCATGAAAGTTTTTTGGAAAAATTTTCTGATGATGCTGGTGTGCATATTTGAGATGATCAGTTTTTTGATAGATTAGAAATAGCATTGAGAAGTATAAGGGCTTGGATAGAAAGTATGTAAATATGTTGAGTTTTAGACTTTTATTATAACACTATGCGCAATACTTGAATTGAAAAAATAGATGAGAAAATCCCCTTCTCCCCCCAAATTAATGCTGTTATAGATCAAATAAAGCGATAGAACCACTGATTTGATGTAGTAAAAATCTGACAATTGGGAAAAATTATAACAACGATAGAGTTCTTGATGGGGATAGTTGGAGTTTCTCTATGAGATTTGGAAATGGAGACAAGGTTTCTGCTTATGGATATATGGCTAGTCCTACAGGTTTTCGAGAGGGCATAAAAGAGTTTTTGTCTCTTTTTGAAGAAAAATCTGCTCGAACTACAGAAAAAGCTAATGAACTGTTAGAGACAATAGGAAAAAGGCAAAGTTAATGTGAGGAGCGTTATAAAAAGGCTTGCATTTGTTGTTAAAGTCCTTGTGTTGTTAGCATAAACTTTATAATCTCAGGGTGTTCTTTTTTATTTTTTATTCTTTTCATCATGAAAAAACGATCAGGAATCAGTCTTATGCTCCTTTTTTTGGGGGCGACTTTATTTTCGGGGTGTAAGGAAACTCAACCTACTGAGCCGGAAGTCAAACCAGCTACAGCATCTCCTGCACAAGCATTTCTAGAGACGCTTGCACCAGTATTTGGCAAAACAGCTAAAGACATTAAACCCTTAGCCTTTAGCGGAGTGTGGATAGATGGAGAACTCAACGGTTATGTTCTTCGTAATGATGCTCTCGCTTCAACTGAAAATATCTGGGACAAGATCGAGCAGACTTTTGAGGGTTGGGTGGAAAAAATCCAAGCTGATGGCGTATGAGAATCGTATGTAGAGTATTTGAATGATACTCTCATCTGTTCTGTCTCTACTAAGCTTAATTACGATATGGATGAGGTGATGAACCTGGTGTACTTAGGGGAAGAAAATGCTGCTAAAAAGCAAGAAGCGACTGAGAAGCTTGATAAGATTTATGATGAGAGCACGAGAACCATTGAGGTTCATTGTGGGGAGTTTACTGAAAATCAACCTTCTCCGCTTGAGTTTAATTTTGATCTCTTCGGAGAAGAGCCATTCTGGGATGCAAGCCTAAGAGTAGATTCGTTTACTTATACGCTTCCAAATAATGAGACACACGCATTTGAAACTGATAATACGTATATCGGCTATTGGGGAACGTCTGGAAAGAATCTGATTTTTTCTGGTGAAAAATATGATCTTGGTGTTATAGAAGGTGAACTCACTAAAGAAGCTTGTCTCGATACGACTGTTGGTGAGACGCATCCTTACGAAGCAAGAATTAGCTTCTGAGATGGAACGTTTGAGGGGTGTGCAGATAAAGCTGATGATTTCCTCGTTCCGGGTAGACAGGGGAAACTCGAAAATCTCCTCAAAAAAATCAATTACAAAGACTATACTTGAAAACTTAATCCAAAAACTGCATCCTATGGAGGTATTCAGGCGGAAGGAAATCTTGTAGAGGTTTTCTTGACACAGCCAGGGAGTGAGCAGACTGAGTACCTTGTGCTAGGAAAGACTGATAAGGGACGAGAAACCTATTGGCAAGGAACAACTCCTGTAGATGATGCAACTTGTGAAAAATATGTGAATGTTCCTAGATTGATGGATTTTTGAATCTTTTCTTCATGTCCGAGAGGATAGGCGTTTTTGGATTTAAGGCTTTTGAAGAGGAGAGAGATCAAAAAGGATCTCACTTTGGCATAATGGCTATTTTGATAGCAAAAAGAGGAAAGAGCAACTTTTGCAGGATTTGCAGAGGGGGCTTTTTTCTTTTTAGTGGAAATGTCGGATGGGTAGTTGGGGAGATCTATTTTTGCTTTGCTCTCAACCTAAAAATGTGGGAAAAAAGCTGAAATCAGATTTAGGACTGAAGGAGCATCCTCCCTCTGTATGAAAGTGGTGTCAGACCTAGGGTGGATTTCTATCAAAAAGCTCAAATTACTCTCAGACCCAGGGTGGATTTCTATCAAAAAGCCCAAATTACTCTCAGACCCAGGGTGGATTTCTATCAAAAAGCTCAAATTACTCTCAGATCTAGGTGGATTTCTATCAAAAAGCTCAAATTACTCTCAGACTCAGGTGGATTTTCATCAAAAAGCTCAAATTACTCTTAGACCCAGGTGGATTTCTATCAAAAAGCTCAAATTACTCTCGGACCTAGGTGGATTTCTATCAAAAAGTCGAAAGTGTGGTAGGAGGATCATAGGTTCCTGATTTTTTTACAAAAAAGCTATCAGAAACTGATGAAAATCTCTTCAAAAGTTTGAAATGTTCAGATTTTTTCTTCTTGTCGGAGGAAAATGAGCAAACCTTCCCGCACATAATGAGTGTAGGACTTCATAAGCTTCTTTGGTTTTGATTCCTTTGCTGGGGGGAGGGGAGTTGGAAGAGGGAAGGCAGGCTTTGGGGCTGTTTGATGATATTTGAGAGGAGGAAGAATAGCGGTAAATGTGCTTGCATTCGTTCTTCGATTGACTATCGTGAGGGAGTCAGATTTCATTTCTTTTTTCTTTTTTATGCTCAAGCCTTCTGCGATTGCTCACTTTCCAAATACGCCTGGTGTTTACCTTTTCAAAGATAAGAAGGGGCAGATTCTCTATATCGGGAAAGCAAAGAATTTGAAGAAGAGGGTTGGGCAATACTTCACTCCAGGGAGTGTTTGGAAGCAGGATATGATTGCACAGGCGGAGACCGTGGATTTTCTCAGTGTGGAGAATGAGAGTGAAAGTCTCTATCTAGAATCTAATCTCATCAAGAAGCATTTGCCTCCGTTTAATAATATGCTGAAGGGGGCGAATGCCTATGCTTATATTAAGCTCACGAAGCATCCTGTTCCTCAGCTTTTCATCACGAGAAAGAAAGTAAATGACGGAGCGGTCTATATTTGACCCAAGCATAATACGAGGGAGCTCAAGAAATTTCTTCAATATCTCAGGCAAATTGTGCAGTATAGAACCTGTCCTTTGAGTCAATTCAAAGAGGGGAAACTGTGTTCAGATTTTTATTTTTGACTATGTAAAGGGTGGTGTGCGAAGCCGGAGTTAGAAAAGCCTGACTATCCCCAGTTGATTACGAGTTTTTTTAAAGGAAACACTCAGCCCATAGAAAAACAGCTTAAAAAGCTGATTGATGATGCTGTAGCGGTGCAGAATTTTGAGTGGGCGGCAAAATTGAGGGATATTTATTTACAAATTTCGCAGTTTACTGAGAAACAATCTGTTGAATATGCGAAATCTTTTTCTGGTTATTTATTGCAGATTCGTCAAATTGGGTTGTGGAGGTGTTATGTGTTGTTGCATTTTTTTGAAGGGAAGATGATCGATGTGATCCGTCATCACTTTCAAGAAGAAGATTTCGATACCGACGAAATGTTGGCGAATTTTTCTTCTGAGTTTGGGACGTTTAGTCCGCAAGGAAATTGGTTTGCTACGGAGAAGTTTTCTCTTACCAAAGAAGAAACTGCACGCATGGATCAGCTTTTTGATGACTTTTTTCAATCCTATCTTTTGGCACAGACTTTGCAAGGAGGGAGTGTGATGAAGGAGCTGTTGACTCAGCTTCAGCAGCGTTATCATTTATCGCATTTCCCGTATCAGATTGAATGTTTGGATATTTCTCATTTAAGTGGGGAGCGGACGAGCGGAGGATTGAGCTGTTTGGTTGGCGGGCTAGAAGAGAAAAGGAACTACAGGAAATACAAAATTCAGACTGTGAAAAATGATGATTATCAGGCATTACAGGAAGTTTTGGTCAGGAGATTTGGGTTGGCGGAGAAACAAGAGGTGGCGAAGTTTGTACTACCGAATCTTTTTATTCTCGACGGAGGGAAAGGTCAGCTTTCAATTCTTTTTGACTTATTAAAGCAATATCCTGCGATAGAAAAGCTTTTTTCAGAAGTGCAATTTGCAGCGCTTGGGAAGGGAGAAGCGAGGAAGAAGTCCCATATTGGGCAAAAGAGTAAAAAGTCTGATTCCACGGTAGGAGAGAAGTTGTATGTCTGGAAGCATTGAAGTATTGAGGAATTTGATCTGGTGTATGATGAGGCAGATCGTTTGCTGACGAAGGTTAGAGATGAGGCTCATAGATTTGCGAACTATTATCGTAAGCAGCAAGAAAGGTTGGCATTTAAAAAAGCCCAGGAAGGGCTTTAGGAGTTATTTGTTTTTTTTTGCTCTTTATTTTGTGGGAGATCCGTTGGATAAGGATTGGTGCGAGGGAAAGGAGGATAATGGCGAAGATAATCAGTGTAAATCTTTTTTGTACGATTTCCAAGCTTCAAAAGCTATAGCCCGCATAGATAAATAATGCAGATCGTAGAAATCCTCCGATGATATTGAATGAGATGAATTTTTTGTATTTCATTTCTCCGATCCCGGCAACGAAAGGAGCAAAAGTTCTGATGATGGGGATGAATCTAGCGTAGATGATGGTTTTCCCTCCGTGTTTATCGTAGAAATGTTGGGTTTTTTCTAGGTGTTCTTTTTTGATTCGTTTGATTTTCCCGGTGAAGACTTTAGGTCAGAGGAATTTACCGATTTCGTAGTTTACGCTATCACCGATAATGGCCGCAAAGAAGACGATCAATCGAATTAGATGAACATTGATCGTGTTTTCTGGGTTGCCTGCTAAGGCACCAGCAGCAAACAATAAAGAATCTCCAGGCAGGAATGGCATTACAACTAATCCGGTCTCGATGAAGATAATCAGGAAAATGATTCCGTAGATTCGTATTCCATAACTATTAAACAGCTCAACGAGGTGTTTATCGATGTGGATGATGAAGTTGAAGAGGTATTTGATCGCGGAAAGGAGCGTGTTCATTGATAAGGTTTAAAAAGAAATAAAATTTTTATAGTGGTTTTTATCTGAGTTCTCGCATTATAATTGCCGAGCTTTGACCAATATTCAGGCTTTCTTTCACTCCTTGCATAGGAATAAAAACGACTTCATCTACTGCTTTTAAAGTCTGAGGAAGCACGCCTTCTACTTCATTTCCGAAGATGATGGCAAGGGACTGTTTTCCTTCTCTTTTATAGTTTGTTAGAGAAACTGCTTCAGGAGCGATTTCTGCTGCGATGACCGAAATTCAGAGCCTTTTCGCTTCCTGAATAGCGTCTGTGCTATAGTTGAACTGTTTTAGTTGCACTTGTTCTTCTGCACCGAGAGAGGTTTTTTTGACTTTGGGATTTTCTACTGGAGAAGGGGAGTATCCGGTTAGTCGGACTTCCCAGCCGAGAGCATCTGCGGTTCTAATGCAATTTCAAACGTTGTATGCGGAGCGGATATTTTCTAGGATGATAATTTTTCTTGGTTGCATCTCAGGTTGTGGAAAGGAGTTGAAGTTGAATTTGAATTTTAGAATCATTTGTTTGTGAGGGGAGTTGTCGGGCTATTCAGATTTAGTCTCTTTTTAAGATTTTCATTTTCTCAGGTGATTGATGGACAAAGAGATCAATAAGATTATTACTGATGATAAACGAGAAATCTGGTAAAATTCCTCCTGTATCAAAGGTAAGCTCGGTATTGCTCTTGTATTCTCTAGAGAAGTTCTCTTTGATAGCTTTAGAAGAAGGTTGGATTCCCTTGATCCCTTGAAAAGATTTGCCGTCTTTTTCGATATTGATAGGAAGTAAGAGCAGATTTTGTGCCTGGTTTCGGATCGGAGTTCCTAAAAGTTTGGAAGTGCCAGGATAGGTGGTTGGGCTACTTTCTATGAGGGTTCATGAGAGCGATAGGAAAGAAAAGAGGACTTTCCCTTTTTCTTCTCTGATTTTGAGGAGTGAGAGTGGAGCTTCGTCCAGTAGGAATAGCTGCTCTTCTTTTTTTGTGGAGAGCGGGATTGGAGCTGTCTCACCGTTTAATGTAATCAGCATTCAATCTTTATTTTTGGTCTCTAGCAGAAGGGTTTCTCAGAGGAGCGTTCCGTGGCTATATTCTGTAGTTCAGCTCCAGATTTCTTGTTGTTGAAGTTTGGAAGTTTGCCCGTTAAGCTCGCTGACTAAGTAATTGTGGAATTTCCCGCTAGAGCGTTTTGTGATGATGCCAACTTTGTTTTGATTGGAGAAGAAGGCGTGATCAATGATTTCTCCGCTGAGTAAGACAGATCTTTGGTAATTAATTTTAGGCTCAAGTCAGAAGATTTGAAGCCTGGTTTCTTGAGAGGGAGTCGTGGTTGCGGCGTAAAGATGATTTTTGCTAAAAATGAATTGATTAAAGTTTCCTAGAAAGGTATGACGTTCTTTTGTTGCTTTTAGGCTTTTGAGATCGACGCTGATCATTGTTCGGAACTGAGGTAATTCCTGTGGAGTGAAAAGCGTTTGTAGGTCTTGACAAGAAACTGCTTTTTGCTTTGTAGAGCCAAGTCCATAGCCAACTCCTTCATTTAGTGTCGGGAAAAAGTTTTTTCCGGCCTGGCGCTGTTTGATGAAGGTAGCAATTTGTTCGCTTGTTGTATTCTCATTGGTGATGAGGTAAAGTTTTTCGTTTTGAACTCAAAGATAAACGCGTTTCCCACTTTGGGCAAAATAGTGTACAGGAGTGATTTTATTATCTAGAATCTCGTAAAACAGCACGACTGTTTCAGACTTTCAGCTTTTACCTTCATGATTTGCGGAAAGAATGAGTGTATTGTTGTATCGATTGATTTGGAGTGCTTTGAAATCTTGTGGAATCGCAATCCTGGCAATTTTTTTTACTTTTGTGAGATCGACGAGTGGAGAGTTGAAGCTTTTTGGTCATTGGATGACCGTTAAAATCTGTGTTTTTTCGTCGAATGTGGTCAAAAAAGTATTTGATTCAGATTTTTCTGTTCCTAGATCGATAGTTGAGACTTGATGACTTTCTGTATACAGTGGAGAGGGAAGAGGAGTACCGTTTTTGACCAATTTCTCGAATTGATTACTGAGATCGATACATGATTTTACAGGTTTTCGATCGGTTTCTGGCTTCTCATTAGCGTTTGCTTTGGTGAAAACTCCGCAGATCAAAACCCCTAAGAGAAAAGATATTTTTTTTATCATAAAAAGAGTGCATAAGTAAAATTCCTTGCTACACTCTTATCGTTTCTGAATTGTTTTTCAAGAGAGAATTAGTTTTTTGGGAGGTTTTATAGTGGCTTAATTGTATGTTTCGCTGATTTGCTGTTCCAAGTTTTTGATTCTGTTTTCAAGTGTTTCTATTCTTTGTTCAATCGATGGATTGCTGTCTTGCTCTTCTGAGACTCTAAGGTCAAAGGTTTTGAGATTCTCTCGACCGCGGTAAAGCTTCATCCTTTCAGAAGTCATTATTCCTTTTTGCTCAATCCCTTTTACAACACTTTTAGTGTAACTGATTTCTCCATTTTCATCTACTCTTGCTTCAACTTCTAGAAAGATATTGAACATTCTATCGGAAAGTTTCACGATAAACTTCTGATTATCTTTCCAAAGTATACTATCGAGATTCCAATATTGGCTATCATTTCTATAGGCTAAACTCCAGTCGCGATTTAATCATTCTAGAGGAGAAAGTTTGTCAGCTTTATTATCTCTAATATCATCATTGATTCTGTTTCTGATATTCCAGGTGTCGAGGTTAAATTTGATAGGGCAGAAAAAGGCATTTTCTTCTTCAACATAAGAGATTAATTCAGAACACTTGTTTGCATTCGGATCATAATCATATTCAGAATATCCTCGTTCGTTCATTCGTTCTGGTTTGTGAAGGTTGGTCCTATAAACTGGGTTTTTGGTGTTGAATTTTCGATTTTTGGGAATGTAGAGATCTACAGAATAGATCATAGGAGCAAGAGGCACTACTGCATCAAAAAGTTGGTGATTTTTGTGGGAAATATGAAGCACGTCGTCTTTGATTTCATAGATAAGTTCGGAAAGGTTTTCTGTTATTTTAGCGAAAGTTTCTTTATTGTAAGCATTAATTTGATACTTGAAAACTGCCTTGATTTTGGTTCCTTCGTAAGGAATGTAATTAATACTACTATTTTCTATTATAAAATCTTGAAATGGGTCTATTGGTAGGATGAGATTATTTGGTTCTATACTAATAGTTAAAGGTTGATTTTTTTCAGGGATTGGAATATAAAGTTCCCTTGTGAATTCAGACACTTCTGAACTTTCTATTTTAGAGAAAAATTGAACTCCTGTAATCCCTGCAATAATTATTGCAAAAACTCAACTTATGATTGCAGTGCTTAAAGTGGCTGTATTCATAAGCCTCTTCTTTGATAACGCACCGATACTTCCTAACAACAGTAATAATGCTGATAGAAATCCAAAAGCTATTCCTAGGGAAGTAATTGCTGGGAATCGGGAAGTTATCTCCATATTATTCCATACATATCAAGTTTGTAGGTAATAGAACATGATTCCGAGTAGGCCAAGGACAAAGACAAGAAATATACTTCGGAAAAAGAGGAAACAGATTTTTATGAAACTCCAAAAAGGTCAAAAGAGTTTTGCTCCTCGTTTTAAGAGCGTAGGAATAGCCGTTCATACAATTCGTTTCACGCTTCTGAATCAGAATTTTATACTATTGCTAATGAGGAGCCTCAAATCCCAAATTTGAGTCAAAAAGTAAGAAAAAACTGATCTTTCCCCATAGTTTTTCTCTGTGATTGGAAAGATAAGAGCAAGCAAAAGATAAGCCATAACACCCCAGAAAAAGAGAATTCCTGTTCCGCTTGTGAGAAATACATAGCTTGCAAATAGCGTTAATCCTCTCCAAATCCACACGTTTCGTCCACTTATTTGTGCAACCATTCCACAAACTCACAAAAGAATTGCACTCTCTTGAGGTCTGTTTCGATCGCTTTTTTGGAGTTTTTCGTAGAAGAGTTCAGGTTGTTGTATTTTTTGTTCTTTATCTGTGCTATTCTCCTGATGGCTTGAGGAAAATTCTTCTTCAGCAAAGATTTCTTCAGGTTCTCCTAGGTTATTTACGATTTGGATTGCGATCTTTTGCGTGATTCAGGTTTTTTCTGTTGCTTTTTCGGCGAGCTTGTCTGCAAGTCTCTGCAAAATATCTTGATGAAGGTCCGTATCAATTTCATTCTTGGCTACGAAAGTATTGATTCTCTCTATATAATCCTGCAGGAACTTGAAAACATTATTTTCAAGTTGGAATGATTTTTCATTGAGATTGAGTGTTTTCATTGGTGTGACAGGTAGGAAAGTAAAATGATTTATCGTGTAAAGTTCAGAATGACACGGGAGATTGATTTTAAAAGAGGCTCCTTTGTCTTGTTTTATAATAAGAGGGATTTTTTTATAGATTCTGGATTCAGAGAATGGCGGTATTGAGCTGATTTCGGATTTGTTCCATCTTTTTCATAACCTGTTTGCCGAGAGGAGTCAGTTTATAATATTTTCTTGGAGGGCCACTGACTGACTCTACCCAGGAATAGGTTAAAAGCTGATCTTTTTTGAGGCGAGAGAGGAGTGGATAGAGCGTCCCTTCTACGATGAGGAGATCATATTTTTGTAATCCGCTGATAATGTCTGAAGCATAGACCTCACCTTTACTAATAATGAGGAGAATAAGATATTCTAAAATTCCTTTTCTCATCTGGATTGCAATATTATCGGTATCATACATCAGCATTCGCTTTTTGAGATAAATATACTACCTTGTATTACAAACTACTATATGAAAAATTTTTTTAATTGCAAGAGCAATACTATTAAAATAGAAACCAGGTAGCTTGTCTTGAGTTGTGAACGGCTTTTTAAGAGCTTTCTAGGAATAGATCAAAAGCTTTTTGGGCTGGGGTATTGTATTCGACAATCTTGATAATCATGTCTAATAGCGGGAATGCTTCGTTCGTAGTTGCGAGAAATTTGGCAATAGCTTCTGTTAAATAGAGGCTTTCTGCAACCATATTTATCAAGCCTCCTGTAATTTCTGCTTTACTATGTCCTTCTGCTAGTAGGCGTCCAGCGTTTCGGTTTCTGCTTAATACGCTTGAGCATGTAACATAGAGATCTCCGATCCCCGCAGAACTTTTCCGAGTTTCGGGTTTGATTTTTAGAGAGGAGCCGATTTTGCTGATTTCTTGGATTCCCTCTGTCAAAATGAGTGCTTTGGTATTTGTGCCGTATTTGAGACCATCAGAGATCCCGCAAGCAAGTGCAATGATATTTTTGAATGCTCCCATAAGCTGTACTCCGACGATATCTTCGCTTTCTTCACATTTAAACCGCTTGTTGTGAAACAGATTTTTAATCTTTTCTCTTCGTTCTTTGTTGGATGAAGCCAAGACCACTTTGGTTGGCTGGTGTTTTACGACCTCTTCGGCGTGAGAGGCGCCTGAAAGCACGATAATCGGCACGGCACCTAGTTTTTCTGTAATGAGGGAGGAAAGAGGAGAAAAGTCTGATGCGTTCCAACCTTTCGAGGCGAGAACTATAGGTTGTCAGGTATAGTAAGGCTTGAGCTCCCTGAGGCAGGAAGTGAGTCCTTTTGATGGGACTGCCAAAACGATAATTTCTGCTCCAGCTAGTGTTTGGGCTAAGTTTGTCGAGCAGCTGAGTTTTTCGTGGAGCTGGACTCATGGCAGATACTCAAAGTTTTCGTGCTTCTGGTTAATCCTGTCGGCGTCTGCCTGAGAAAGTGAGAAAAGTCTGGTTTCGTGACCATTCTCGGAGAGCACTTGTCCCAGTGCTGTTCACCAACTGCCTGCGCCTATTATTGTGCATTTCATTCTCCTTTTTTTAAGAATAAAAGCTAAATTTTTTATGCGATTCGATGGATTTCTGCTGATTTTCATCTTTGTAAGTATCGGAGGAGTCTTTTCTCTCTGAGTAAGGAATGGTTCTGGTCGGTGGCGAAAATTGAAATGGAGTATTCTGATTTTTCTTTCTTTTTTGTCAGTTCGAGGAAGATCTTTTCGAGCTTCTCTTGGCGGTGGAATCTGATTTTTCTGAAATTTAGGAATTTAGCGATGGTTTTTTCTGCGTTGAAATAGAGGTAGTTGAGCGGAATGAGTGAATCGACTGCTGCATCCTTGAAATAGCGGAGCTCGTGCTTTAAAAGTGTATTAGTAAGAATGCTTTTTCGGGAGAGTCTGCTAAGGAGCGGATTGAGGATGCCGCATTCTTTGGATCGGTACTGGAAGGGAAAGTGAAAAGTTTTTGTGGTTTTTTGTGCGGGAGTCGGAGTATAGGAGCTGGAGAGCTCAGTTAGTAAGGATTGGGCTTTTTCTAAGTCAGCATATTCTCCAAGAATTCCTAATTTTCCGACGAGAGAGTGAGCGAAAACTTCTGTGAGAAGGTAAGTCTGAAAGAGGGGAGCAATCAGCTTTTCTTTTTTTTCTTGTAGGAGATAGAGTTCATATATAGGTGGTAGAATTATGAGGTCTACTTCTTTATTTTTTAGTCGCTCGATTCCTTTTCTGACGGCTTGTAAGGAGGTTTCAAAGTGTTTCTCTCCATAAGGGGCGTTGAGACTATCATAGTAGATGAGGTATTCTTGGTCGTGGCGGTGCAAGAAGCTGAAGAGTGCCAAGCTTTCCATTCCACTTGAGATGATTCCTATTTTCATACCTCTGGCAAAGGAAATAAAGTTAAGTAGCTTTCAAAGTTTTTCATAAAGGTATCCATTGAGTCTGCTGTGAGTAGAAGATTAATCATTTCTAATTTTAGGGTGAGCTCGGAAAGTGTTTTCTCGAGATTAAAGGTATTTCCTGAGGCTTGTGCAAGCTCTTTTTCAGTTTCGATGATTGCGGTCTGGAGCCCTTGCTGTTTCTTAAGGAGGGTATTTTGGAGGAAGAGTTTACTCTTGCTTAGTAACTCGTTCTCAAAATCTTCCATTGCTCCCTGGATCATATTCTTCTGTGAAGCGAGCTTTAAGAAAAGCTTTTTTTTGGATTCATAGAGCGAAGATCGGTAGGAGCGGTCATCTCAGCGGTTGAGGTTGTTCATGATTTGTGTGAGGTCCTGATAAGCTGTTTTGAGGTAACTATTGATGACGGTAAGGTATTCCTGGCATTTTAAGGTCTCACTTTCTTGGACGAGATATACTCTGACTGCGTTTTCGGTCACTTTACAGCTCAGCTTCTTCTGGAAATTGGAGGCTGCATAGGTCGAAGCAGTGCCTCGTAGAAGGGATGAGAGCAAAACTCAAAGGAAGATCAGTTTTTTCATACTTTATATCGTTTGATGAGGTAAAAGTGGATTTCTATTTTTGATGTTCGGAAAGCAAGATGCTTTTTGTGGCTAGTCGATAATAAGATTCTGGATACTTTCAAGTTTTGATAGCAAATTCGAGTTCGATGAGCTGTTTAAAGAAGCTTTTGATAAAGTCTTGGTAGCTAAGCAATATGCTAATGTTTTTTAGGTTTTTGTGGACGACAAAAGGGTTAAGCTTGGTCTGTGCGATGATTTCTTTGGCTGAAGTGATGCCTTGTTGATGATAATCTAGCAGCGTAAGATATACTTTCAGTCCTCGGGTCAAAAGTCCGTTCGCGGTGTTTCGGTTTTCTCCGTCGCTCTTCATTTTTTCAAGCGTCTGGGCTGCGAGCACCGGATCAGAAAAAAGCTGATCAAAAAATGCAAAAGAATCTCCCTCTACCATTCCAAAACAGTATTCATCGATCTGTTCTTTGGTGATCTGACTGGTCGCTGGAGGGAGTCAGTATTTTAATTTTTCTGCTTCGCTTACTAAACGGTAAGGATCGTCGCCGATTTTTAGGAGGAAGTAGCTTAATTCTTCGTTCCCGATCTTGAGTGGCTCGAGCTGACTGGTAATGAGCGTTTTTAGTTGTGGTTCTTTGTAAGGTTCAAAGTTTTTGAGCTGGAGATTGTCCATGCATCGTTTGAAAGGTTTGGTTCTTTTGTCGGGTTTATTAGAAAGGAGAATCACGATCGTATCTGCTGGGAGTTTGTCGATATTTTGCTGGAAATCCTCAAAAAATTTCTCAATTTTATCTGCTGAAAGGGTATTCCCTTCTGAAGTGTCTTTTGGTACTCCCTCAATGATTACCATTTTCTTGGAAACGAAAAGTCCACCAGCATAGAGTGCCTGCTTGATCATCCCAAGGTCTCGGTTCTGATTGGAAAAGTGAAAAAGTGCATCTTGCCCGTATCTTTCCAAGAAGTTTACTTTTCGCCTTTGGAGTTCTTGTCTTAATGCGTAAGTATTTTCGCCATAGAAAAGGAAAACAGAAGCATACATTAGGAATTAAGGTTAAGAAGTAAGGGACTTATCAAGTATCATGCTGTGTTGGAATTGTATTTATTTTTTGCTCGGTTTCAAGGAGTGATTTGTGCAGAGCGGGGTGAGCGTGTTTTCTTTTGCCTATGGGGCGAGCTCTTGTGAGAGTAATCCTTCCGGAAAATGAATAAAAAGTTCGTTGACCTCATCCCAATCGAAATTTTCGCTTCAGATTTTTTCTTGTATTGCTTGTGCTCAAGCGTTACTGTCCTTGCTATAGTCTTCAAATCTCAGCTGGAGTAGCGTTTCGAGGTCTTTTTTAAGAGCAGAAAAGACTGAATCCTCTCCGTAGGTTATCGTGATTTTTTCGACGGAGGGAATCAGCAGAAAGAGGAGCGAGGCACGATAGAGGAGCGTCTGGTTCCTTTCTCTGTCGCTTGAGAAGTCAAAACCGATATTTTTTCCCTCACTGGCATTGAAATGGTTGATGGCAAAGGTGTAGGGTCTCTGTTCGGTGCTAATCGAAAAATCAGCATACTCACTGAACTGTTTTAAGAGCTTTTTGATGGTGGCGGTGTCGGTAAGTGCGGTTTGCTCGTATTTGGTAAGTCGCTGCTCGGCAGCTTGAGGAGCTTTAGAAGCTTTGGGTCGCAGCAAGGTCGTGATTCCGATGAGTAGGATTCCGCCGAGAAGGAGCAAAGCTCGCTGTATGTTTTTCATAATGTATTTGGGGAGGTAAATCGTCTTTTACTATGGAGTTTCGCTGTTAAAAATCAAGGATAAAGTCAAATTTTCTTTTGTTTTGTTTCAAAAAAAGCTTTTGAAGGCAGATCGTTCCTCTTGGTTAGGGGTTTTGATCCGAGCAGTTTTGAAATAGGGCGTTTTGGTAGTCGCTTGATGCAACAAGGTACAAAAATGCCTCAAACTTGGATCAAAAAGTAAAAAGTGATGTCAGATTGACTCACTCTAGCACCACCTGAGAGCAAAGTGATGCCAGATTGCCTCACTCCAGCACCACCTGAGTGCAAAGTGGTGTCAGATTGCCTCACTCCAGCACCACCTGAGAGCAAAGTGGTGTCAGATTGACTCACTCTAGCACCACCTGAGTGCAAAGTGATGCCAGATTGCCTCACTCTAGCACCACCTGAGAGCAAAGTGGTGTCAGATTGACTCAAAATTCTAGTAACATCTCCAAAGCATTGATTTTTTGTCTGAAGGGGGAGGGAAAATGGAGGAAGGATTCAGATTTTTTGAGTTTTTTCATGGTTTGGGAGGAGGGGGAGGTTGGAAAGTTTGGATTTTCTCTGAGTTAAAGCGGGTTTTGACAGAAGCAGAGGTTGATTTTGGAGTTATAGGGGCTTGGGAGCCGTGTGATCTGGGCGAAATGCGTGCTGAGGAGGGATTGCAGATGAGGGAATTGTGCTGCTTCGGATTTGGTCAGGAGATGGACTTCACAGAGGAGGGTTTTGATGCAAGATCGGGTCGCGGGCTCTAGAGCAAAGAGGACTTCGAACTCCATTCCTTCGATATCGAGCTTTACCAAGTCGATGGATCAGATTTTTTGCTCTTTGATATACTGATTGAGTGATTGCGTATGGATCTGTTGAGGAATACCTGCGGGATTGAGAAAAGATGCCGCTTTACTCGACTGCATGGTCTTGGATGCATTGAAGGTGAAGGTTGCTGTGCCTGTGGAGCTAGCGATAGCGAAGGGATTGAAGGTGATTTGAGGTGCAAAGTCTTGAAGTCTGATTTTTGCCTGCTGGAAGAGGGCGTCAAAGGGTTCGAAATAATGGAGCTGGAGGTGTGGATTGAGTGAAAGGCATCGTTCAGAGAAAAATCCGAGGTGGCCGCCGATATCAAAGATGGTTTTTGCGTTTTTGATGGTAGGCTTGGCGATGCGGTACTCCTCCTTGATGAAGATTTCGTAGTAGAGGGCTTGCTCGTATTTCCCGAGCTGGGCAGAGGAAGCTAGCATGGGTGGTGTTTGGTTAAAGCGGTGGAAGGATTGAGAGAGCTATGGAGGGAGGCTTTTGTTTATCGGCCTCTTCTTTCCCCTGATTCAAGGGCACGGTATTTGGTGCTACAAGAGATGATGCCGCCGATGTTTACTACGAAGAATAGTGGAATATAGACGAGAGAAAAAGGGATGATGTAAATGATTTTTCGGAGGTCTTTTCGAGATTTGATGAGCGGAATGGTATAAGGGAGTGCAAAGAGGGTGAAGCCTAGCTTGGTAAAGATTGTGGTTAGTCGCCGTTTAGGATTTAAGATGAAGAAGATAGCGGTCCAAGATTGATTTGCTTCTATAATGGTATTGATGAGGATTCGGTCTTTGACAAAGTAGAACACAGAGACTCCCGTGCTAACACAGAAATAACTTATGAAGAGCAGATGAAGGGGATTTTTTATCCAGATTTTTCGATATTTCACAAAGCATTGAAAACCTCATGATCCTCGTCTAATTTTTTGTTTCCATCGGTCTTTAAAGGTATTTGGGACTTCTGTTTCTACTGGGATTTGGCTTTGTTCTACTTTGTATCAGGCTTTATTAAGTTTGAATGCTAAATCCATATCTTCAATAATAGCTTGATAGGAAAAAGAACCGACCTCTAGAAAAGCTTTTTTGTCTACCATAATACAGCCTCCTCGCATTCCCATTGCACTAATGATATTATAAGAACCTTGTATATAGGCTAACATTACATATTCGATGTGTTGCATGGTTGGTAGTATCCCTTTATTAAGTGGTTTGTAAGGGCAGGAAACCCCGGCAACCTTTCAGACTTTTCTCTTGAGCATATCAGCGATATTTGCTTTGGAGATTTTGGTATCAGCATCAATAATGAGTAGGGTAGCATGGCTTGCAGCGGGGATTATTTCGTTTAAGGATTTGGACTTTCCGAGGTTTTCCTTGTTGTTGATCAAGTGGAAGGGGTAGCTTTGGTTGAGCTTCTCGAGGATTGCTAGCGAATTGTCGGTGCTTTTGTCGTTTATCACGAAAACCTCTAACAGCTCGTGGGGATAGCTTGCATAGAGGCTTTTGAGGGTTGTTTCAATTGTTGCTCCGTCGTTGTAGCAGGGGATGAAGGCTGAAAGCGGAGTGCCTTCAATTTTTTTTCTTTTTTTCTGGTGATCCAATCGGAGTAAAGTTCCAATAATAAGGAGAAAACCACATCAGATAAGAATATTTCGCATCCCGTCAAATGAAAATAAATACGCTATCCAATAAATCCACCAGATTGGATATTCCGTAATTTTTTATAGATTCCGTTGTCTTTTGCAAGGAGCTCTTTGTGTGAGCCTTTCTCTGCGATTTTCCCTTGATCCATCACGATGATTTTGTCCATTTTCATAATGGTTGAGAGTCTGTGGGCGATAATTAAGAGGGTTTTGTTTTGCATCAGCTCTTCTATGGCTTCTTGGATAAGGAATTCTGATTCGCTATCAAGTGCTGATGTTGCCTCATCCATGACGATGATTTGTTTATTCTCGAGGATCGCTCTCGCAATCGCTACTCTTTGCCTTTCTCCTCATGAAAGTTTGATTCCCCTTTCTCCAACTAAAGTTTCATAACTGTCCTTGAGATGAGAAATAAATTCGTGGCATCTCGCCATTTTGGCAGCAGCAATGATTTCTTCATCACTTGCGTTAGGTTTTCCATAGGCGATATTTTCTCTGATGGAACGATGAAAGAGAATTGGATCTTGAGGAATCATAGACATTGAAGATCTCAAAGATTCTTGTGTAACTTCAGCGATATTTTGATCATCAATCAGAATTTCCCCTTTTTGAATATCATAAAGTCTGAATAAGAGTTTGGTAATCGTGGTTTTTCCGCTTCCGGATTCTCCGACGAGTGCGACTCTTTCTCCTGGTTTGATATGGAAATTAAGGTCGTGGAAGATTTGGTTTTCTCCGTAGCTGAAATTTACGTTGTTGAATGAGATTGCTCCGTGTTGGAGTTTCAGTTTTTTGGAGCTTTTGTCTTGGATTTCATGCGGAGTGTCAATGATTTCTACAACTTCTCAGATTTCTGTTATCGATCTGAAGAGGCTTCTTAATGTCTGACCAATCCCTTGAAGGAAGTCCATAATTTTCAATACATAAGTTTGCAACAAAACGATCATACCGATTGAGATTACTCCTGCACCTCGCATACGAAGAGCAATATACATGATTCAAATTTGGAGAAAGATAGCAAACAAGCTAGTACTTCCCCAGATTCGCATTGATTTGAAGTATTGAACCTTTCTTGCATGTGTGGCTTCATAGTTTAATTTTGCATATTTTTTTTCTTCTTTTGACTCAGAGGCGAAGAATTTGATTGTAAAATTATTAGTAATAATATCAGATAAAACGCCTCACTGTTCGCTATCTAATGCATTCGCTTTTTCTTGATAAGGATGGATAAAGATATAAAGTTTGTACTGAATAAAAGAGAAGAGAATAATCAGGATCAGAAGACCTCAGGCAAGTAGGAGTTGTTCCTGAGCAATGATAATAAGAATCAGTGTAATATTGATGATAAATGGGATTATTTCCCGCAAAAGAATGTCTGTAATCCTTTCAAAAGCAGACACGCCTTTCCTTAGCTTACTAATTAGAGATCAAGTAAAATTATCAGTAAAAAACTGGAAAGAATGTTGTTGGAGTTTCTGAAGAAAGGTGTTATTTAACCATTCTTGGTTTTTCATCTCAAAGGAGACGAAAAAATATTCATAGATTCTATATACGGTAAAAGTTCCTATTTGGATCCCAAAAAGCACAAAGAGTACGCTGATGGCATGTGCCATAATCTCATTTTGAATTCCTCCTACTTGGGTAATGCTATCAAAAATTTCTTTGTAAAAAAGAGGAGGAACGATCATTAATCCTGTTCCTATAATCCTCGTAATAAGTGCTACTGTTCCATATCCCCAAAACTGTTTGTATGTATAGAGCAGATATTTTATCGTATTCCCATAATTCTTTTTGAAATAGCTTTTATCTGTATATTTTGCAGAGAAAGACATATTCAGTGTTTAGTGTTTTAAAGTCTTAAAGTATCTTTCCATGGAGATTAAAATTTCTTGCTGAGCTTCTAGGATACTTCAAAATCATTTCACTTCTACTTTGTCATATTTTTCGTGTTCGAGGAATTTGATTTGTTTGAAAATCGTGAAGAGTTCTTCCTTCATATGGTGTCCGAGATCATCGATATGGTGGATTTCGTCCCGTCTTGGGTCTACGTCACTTGTTGGAACGGCAATAATTTTAGGGTCATCTCCTGCATTATCGCAAGTATAGAGGATTCCGATAGGTCTTGCCTTGATGATGCAACCGGTGAAAGTTGGATTGGTTGTAAGAAGACATACATCACAAGGATCGCCGTCTTCAGATAAGGTTTGAGGGATGAAGCCGTAATCTACAGGATAGTACATACTACTATGAATAACACGGTCAAGTCTGAAACATCCTAAGACATGATCGTATTCGTATTTATTGTTTGATCCTCTAGGAACATCAACTACGACATTGATATAACCTTTTTGTTGGTCAACAAATGTTGGGACGTCATTAATTAAGTGCATTTTTTCTTGAAAAGAACTAAAATTAGTAGCCCGTGAGTATAGTTTTTTTCTGTTTGATTTCAATTCAAAGATGAGTATATATGGTTTGTTTTTAGCTATGCTATTGTAGGATGCTACATTATTTTCATGGAGAAATAAAAAGGGTGAAGTCTGAAACCTTCCTCGTGAATCCTTATTTCGGGGTTCAGGTTGCTTATGCTGGTGGGCAGTCTCAGGGAGAATTTTTCCTTTATCCTTATTTGGATGATCAAAAAAAGTCAGTTTTCTATTTTGCTTTTGATACTTTTGATCAGAAGGAATGGTTTGAAAGAATGTTGAAAATCTCTGGAATTGGACCGAAAATTGCTTTTCAGATAGTGAAGATTCCGATGCAAGAATTGGCAGCTGCGATTGACCGCTTGGATCCCAAAATTTTTCAGACGATTTCTGGAGTTGGTCCGAAGCTTGCGAAGAAAATTATTCTTGAACTGAAGGGAAATTTTGATTTTTCAGAAGTTGCTGCTGTCAACCAGGATCAAAAGCTTTTTAAGGCGGTAGTAAAGTCGCTTCAAAATCTGGGATACGATGTCCAAGAAGTGAAATCAATTCTAGCTAGCTATCCGAAACCTATCGAAAAGGAGCATCTTGCTGAGGTGGTTAAGTGGGTGATTGCTAAGATGTAATTTCTCTTTATTTCTATTTTTTGCTCTTGGGGATTGATTCAGATTTGTGTTTTTTAGTGCGGGAGTGGAAAATTTTTTGAAAAAGTAATAACTTCTTGCCTTAATGCATCTAAAGTTGCTGGATTTTCGCGGTTGTGGATTGCTCTATCAATGAAGGAGACGATCTTTTTGACTTCGTTTTCGGTAATTCCTCTTGTCGTCATTGCGGCTATCCCGATCCTTATTCCAGAGGGGCGGAATGGAGGACGTGGATCGTGAGGAATGAGTGATTTGGAAACTGAGATTCCAATTTGGTCAAGTATGTTTTCAATTTCAGCTCCGTTAAAAGGCTCTTGTGAAAAGTCTAGGATAACCATATGATTGTCTGTCCCTCCTGTTACGAGTTTGTAGCCTAAACTGAGGAAGGTTTGTGCCATCATTTGGGCGTTTTTGAGGGTTTGTTTGGCGTAATGCTGAAAAGCTGGAGTCTGAACTTCACCTAGTGCGACAGTGATTCCTGCGATAGTATTCATATGTGGTCATCATTGCATTCCTGGGAACACAGCACGATCAATCCTGGTCGGGAGATTCTCTATTGTAGGTTCTGGCTTTTGGAGAGGATTTCAGACTTTCCCTTTACTTAAAATCAATGCTCCTCTCGGTCCACGAAGCGATTTATGCGTTGTCGTCATCATTGCGTGGAAACCATAATCTAGCGGATTCTTCAGCAAACCTGCCGCGATAAAGCCTCCAACATGAGAAACATCAGCATAGGCGATCGCTCAGACTTCTTTTGCTATTTCTGCGAATCTCGCATAATCAAGCTCTCTTGGATAGGCACTAAATCCCGCTAAAATCACCTTTGGTTTCATCTCCAAAGCTAAGCTACGAACTTGGGCAAAATCAATTGTCCCATCAGGCAAAGTTCCATAGCTGTGAAATTTGAAAACTTTGGAAATAAAGGTCACCGGAGATCCATGTGTCAAATGTCATCAGAAATCCATCCCCATACCGAGAATATGATCTCAAGGTTCCATAGTAGCAGTATAAAAGCAGAGATTCGCAGCTGCTCCAGAAAGTGCTTGGACATTGGCATGATCCGCATGAAAAATGGTTTTTGCTCTTTCTATTGCGAGGGTTTCTAGTTTGTCGGTATTTTCCTGTCCTCAGTAGTATCTTTTTCAAGGGAAACCTTCAGCATATTTATTGGCAAAAACTGAAGCCTGAGCCTGAAGAACTGCAGAAGATTGGTAATTTTCACTTGCAATAAGCTCAATTCCCATTATTTGTCTCTGTTCTTCGGATTGGATCAGATTTTGAACTTCTTTATCTTTGATAGAAATTTGCATTTGGGCTTCGGAAAATTAGCTAAAGATTTGATGCTTAGTGTATAGTTATCTAGAGGGAAAAATCAACGGAGATAGAGTTTTATTTTTTTATGACAAGGTTATTTTGTTTAAAGTTTATTTTTCTTTCAATTTTTTTGGCATTTAGAAAGGTAGTAGCTATACTTAGCTATATCTTTATAAACTAAATAACAGATATGTGATTGTCTTCTGAGCCCTCTTCTCGTTGAACAGTATCATCTCATAAAAATTGAGGAGAAAATGCCTCTAATAAATCGATCTTACAAGTTCTTAATCAAAATATGGCAGCATATGAAAAAGGATTTTGAAAAAGTATTATTGTCCCTCAAATAAATGGAGGGTAAAGTGTTCCTGTGTGATGAGAGTCTTTGTCTAATAAAATGAATATATTCTTACAATGCGATTTTGATCATCCACTAATTCAAAACTTTTATCAAAAAGTAGACGAAGGCTATAATACTGCTATTGAAGATCCAAAATTCAAAGAATGGTATGAAACGAAGAAGCTACCTATTCGTGGAGAACTCTTTTTGCGTCTTTCTGCCTATACTTCAATATTTAATAAAATGTTCACGAGAAAAGAAAACTCTGCTCGAGAAAGGCATGAGTATTATAATAAGCAGGAAGTTGCATTGCTATCTGAAATCCTAGAGAAAGGGATATTTATGTGTGCAGAACTAGCTTCGTTAGCACATTACTATCTTAAGACTCATGGGGTTGACAATTTTTATGTTTGATGAGAATTGCTTCCAAAACTTTATGAAGAGAGTGAGGAATTGCGGGGGGGGAGGCACATAGCTTTATTTACCTTAGAGACAATAATATTCAACTTATTTATGATCCAGCATTTCCTATAAAAACATCAAATGCTGGTTTTCTTTTTCCTAAGATCCAAGAAATTTTAAACGACTCAGATTTTCTAAAACTTTTTAGAATGAAGAAACAACAATTTGTTGAAGCTAAAGACTTATTATGCACCGATTCAAAACTTTATTATGGATTTTGAAATTGATCCTGTATTTATCCAGAGAATATTCTTTTGTGAAGCGAATCTAAACAACAAAATAAAGATTAGTCTTTAATGAGTTCTCCCCAATCACCTTGCTTAAGCCCAGCAGCATTCCCTTCTTCTACATCAATATGCATATCAAGAGCATAACTATCTGAAACTCTAATCACTACCTCATCAAAAATCAGACTTCTTTCTCCGTTGGTCTTTACCTTCACGATTTGTCCGTTCGTTACCCCAAAATTCTCTGCATCAGCCACCGTCATATGAATATGTCTCTTGGCAACAATCATTCCTTGAGTAAGCTCAACCTGACCTGCCGGACCAATGATCGTCACTGGAGCTGATCCGTCTAAATCTCCTGATTCTCTGATCGGAGTTGGAGTCCCAAGTTTGAACGTATCTCCGAGCATCACTTCCACCTGAGAAAACTTTCTATACGGTCCTAAAATTCTGACTTTTGAGATTTCTCATTTCGCACCTTTAATCGTTACGCATTCTTCGCATGCAAATTGTCCAGGTTGAGAGAGGTCTTTGAGTTTTGTGAGCTCATAATTAGGTCCGAAAAGGGTATTTGCATCAGCCTGTGAAAGATGCACGTGCCTGTTTGATATTCCGATAGGGATTCTCATTTTTTAAGATAAGAAAAAGTAAAAAGTGTTGCCGTGAGTATAGCTAAGTATTCAGTGTTTTCAAGGAAAAGAAGCAGGAGAAGTCAGATTTTTATTTGTAGGCGTTTCGAGCGTCTTCGACGGCAGTGATGATGGTTTTTTGAGGTTCTCGTCAGAGCATTTTTTTTGCTTTTGAAGGATTGGCTACAGAGATTGCAACGTCTCCTTCTCTTCTCTCTCCAATAGTATGTGCAATCGGTTCTCAGACGATAGATTTGGTGATTGCAAGCATTTCTTTTACTGATTTTCAAGTACCTGTCCCGATATTGAAGGCTTCAAAGAAGTTTGGAAGTCAGTTTTCTACCTCACTACTTTGCTGGATGTATTTTTGGATATAGTCAAACGCTTGTAAATGAGCCTGAGCAACGTCCTCTACGTGAATATAATCTCTAATGCAGGTCCCATCTGGCGTGATGTAGTTTCCTCCAAAGATCGTAATTTGCTCGATCTCTTTTTTTGCCACTTTCATGAGGAAAGGAACGAGGTTTGTCGGAATTCCTTTTGGGTTTTCTCAGAGAAGACCTGAAGAATGTGCCCCGATGGGATTGAAGTATCTGAGGCAAATCGCTGAAAATCCTTTGTGGAGTGCCATATCGTAGAGAAGTTTCTCAATGACGAGTTTAGTCGTTCCATAAGGGTTGGTGGTGCTAAGGCGATCAGTTTCTGTAAACGGTGGGATATTTTTTTCAGTATCGTATACTGTTGCACTAGAGGAGAAAATCAGCTTTTTAACCTGATGCTTGTTCATTACTTTGAGGAGATTGATGCTTCCAATGATATTGTTGTCATAATAGAGGAAAGGATCATAGCAACTCTCTCCCACTGCTTTTTTCGCAGCAAAGTGAATTACTCCGTCAATCTCTTCTTTGGAGAAAAGGTTATCAAGATAATCATAGTTTCTTATGTCTCCTTCGTAGAAAAGAGGTTCTTTGCCTGTGAGTTCTTTGATCGCGGCGAGGACCGATTCTTTGTGGGCGTTTGAGAGATTATCGAGGATAACGACGTCATATCCAGCTTGTATAAATAGGACTGTGGTATGTGCTCCAATATAACCCAGCCCTCCAGTAATTAGGATTTTTTTATTCTCCATAGGTAAGAGAAGAGATAAAAAGATGCTGTAGTATATTGATACGCCTTATAAAAGTCAAGAACAATGGTTTTTGTGGCTTGCTCTCTTGATTCCCCTCTTCTAGAGAAAAAATGTGGATTGGCTTGTATCTGTAAAGGAGTTTCTTAGATTTGTTTGGTGGTCTTTATTTTTGCTTTTGAAGATGTTTTTTTCTTTTATTAATCTTGGTTGCACGAAAAACCTTGTGGATACCCAATTTTTACTTGGAAGGATATTGGAACGTCGACCTGATCAGTTTTTTTATGCTGTTGATCCTTTTTCTGATGAAGTAGAGCTCGTTTTTCTCAATACTTGTGGGTTTATTTCTTCGGGGAGGCAAGAAATGTTTCATACCATGAAAAAGCTCCTGAGAAAAGAGAAAAAAATCTGTCTCTTGGGATGTGCGGTCCAGTATTTTGAGAAATTGGTGAAGGATGCTCGTCTTTCTGATGCAGAAACTCAAGAATGGGAAAGGCTTAAATCGCATCCTGGGCTTACTTTTCTTTCGTGGTCGGAACTTGCGAATTTTGAACCGTCTTTGCTAGAAAAATCGTTGATTGCTGGAGAAAATGAGTTGTTTGATGGGAACGATTTTCAGTGGCAAGAGAATGCAAGAGCTTATACCAATAGTGAACTAGGTTTCGAATATCTCAAGATCGCAGAATGATGTAATAATCATTGTTCTTTCTGTATTATCCCGACTATTAGAGGGAGACAGCGTTCGCTTTCTCTAGAGGCGGTGATTGCAGAGGCGCGCAATTTGATTGCTTCTGGAGTGAGGGAGTTGATCCTGATCGCCCAAGATAGTACAAGGTATGGAGTCGATCTGTATGGAAAGAGTCAGCTTTTTGAACTTTTGGAAGGAATTGATGCATTGGAGGGAGATTTTCACTATAGGGTGCTGTATCTCTATCCAGATTTGCTTACGAAAAGGCATCTTGAGAGGCTTACCAAACTCAAAAAATTTATCCCGTATTTTGATCTTCCGCTCCAACATGCATCTCCGAAAATTCTCAAGGCAATGGGGAGATTTTACGATCATCAGATGACCCTGGAATTGCTCGATTTTATTGAAGGGCATTTTCCTGAGCGTTTTGTGAGGACGAATTTTATTATTGGGTTTCCGGGTGAGACGGAGGAGGATTTTCAAGCTTTGATGGATTTTATCAGAGGAGATTGGTTTGAGAATATTGCACTCTTTGAATATCATGACGAACCGCTGGCGGCTTCCAGTCTTTTGCCTGATAAAGTAGAAGCGAAAACGATTAGAAAGAGATTTTTGGAGGCGAAAAAATTGGTGGATGAGCTTCAGGCACAGAGAAGCCAGCATGCAGCTAAAGAAAAAACCTGAACCATCTCAGAACTTCGATCGGATGCTCAGGGAAACTGGAGGCTTATGGTGCGTCCTTTCCTTCATTGTCCGGAAATTGATGAGGAAGATGAAATTCAGATTGAGCAGGTGATGCAATGTTTTGATGGGGAGGAACTTACTCTTGGAAGCAGAATCGCCTATCGTTTGCCTTAGTGGGGTTTGGGTGGTTGCTTTGTCTGTGTGGGTGCGGTTGATTGTCCGTAAACGATTCCATAGAGCAACATCAGCGGATATACGACCATCCTATCTACAAAGCTATGCAATACCATTCCCTCGAGTGCCAAGCCAAGCATCCCTATTCCAAATCCTGTGAGGCAAACAAGCTGATTGATTTTCTTTTGGTCAGCTTTTTTTCCTTTTTTGCATTCAAGGGCTAAAGGTTTGATCGCTCTGAGTCCAATTCGAATCAGCCAAATAAAACAGAATAATCGTGGGAGCAATCCTAAAATACCGTATTCCATAAAGATTTGCACATATTGGTTTTCTGGATTGTATCAAAATGTGGGGATTTCGTAGCGAGCGTTGACTTCTTTGATGCGTTCGCATCTTGGGTTTTGGGAGTTGGGGTCGGAACTCTGGAGGGCATTAAAGCAAACCTGGTGGGAGGCCGGTCCTGAGTAGCCTGCACCTCGTCAAAAAAATGGGCTTTCCTGTCAGATTTTGAGCCCTTCGATCAAAAGTGCGGCGTGTCCCGTATTTGAGTGTTCTCTGGCAAAGATTCCCCTATAGTGCCAAAACAGTGCTCCACCTACTAAAGCGAGCGGTAGTCCTATGCCGAAGATGAGTCTTTTGGCATAGGCTCGGTAGAGTGCTAAGATGATAATCGCTGTTTCTAGGAGTCGGACCCCGATCGCTGCCCTAGAAAAGGTCGAGAACACCAAAAGTCCGTAGCCGATCATTCGTAGTAATGCCTCTTTTTTGGAGCGGGTCCTGAGATAGCTCAGGGCGAACAATGGTCGGAATGCAACCAAAAAGAATCCGAAATAGATGGGTCTCTCAAAGAGAAACTCGTTCCTGACGAAGCCTTGATTGATATTGGTATAATAGACTGCTGGCGGTCTAGAGCCTATTGTCCCTTCATAATTAAAACGGTTGTAGCCGAGGTGATCAAGTGTATTGGGCATGAAATAGCTGAGCGCTCGCCAGAAGAGTCCTCCGATGACTACCAGCTGGAGAAGTTTTGTATATCGCTTGGTGATTTTGAGGCGAGGCTGAGGGAGGTATCGTGTGAGCCCAACTCCCAAGAGGAAGATCAAGAAGCCCATCAGATCATACTTGAATGACAAGATATAGGTCCCTAATCCTACTTTTTGGATCCCAACTGCAAGGAGGAAACTAATCCCACACGCTATCAAAAATCAGATTAAAAATCTGAAGAGTTCCGAAGCGCGCAAGTGATTCCAAGTCAGCTTTCTTTCTTCTTTTCCCTTACAGAAAAAGATCCAATAGCCAATAAGCCCTGCAAAGAGAAGAATAAAGATTTCTTTCCAGAGTCGGATTAGGGTTCGGATGCCTCCCTCTCGACCGAGTTGGTGTGTAATCAAAGTATGGAGGAAAAACTGCACGAGCAATCCTCAAAAGAAGATTTTGTAAAGTCGAGGGAGTAGGGCTTGGATTCTTTGCTTCATTATGCATAGGTGTTGGTTAAAACTATCGCTGTGTAGGATAAATAATTGCCCCGAATTGTCAAGGTTTATCTGAAATTTTTGGAAGTAGGGGAGTGCTGGCATTCCCTTAGGAAGCGAGGCGGGCGTTGCTGCCGGCTCCTCACAAAGATACAAAGAAAAGTCTGATTCGAGATCAGGCTTTTTCTATTTATTCATTGTATGAGGGGATTTTCAGGAGAGGACTCAAGAATGCCTATTTCAAAAGGGCATTGATGATTTGAGGTTCGACCTCATCATTGATATATCTTTCCACGGGTCTGGCACCGAAAGCTGGGTCGTAGATTTTATCGATAATTTTATCGACCTCTGCTTTGGAGAATTTTGGCAACTTCACTTGATCGTTTTCTTTCCAAGCAGCGAGAAAGGTTTTGATTTGCTGGTTGAAGATGTCTGCGAGGTTTGCTTTGGTCAGCGGTCTAAAGACGACTTTGTGATCGATTCTATTGGAGAGTTCTGGGGTTAGGAAGTTTTTGAGCTCCTCGAGGACTCTGGTTTTGATGTCGTCGAACTGTTTGGTCTCGATATCGTTTTTACTTCCCGTATCGAATCCGATAGCGGTTTTCTTTTTGCTAAACTCTTCGGTGCCGATATTTGAGGTCATCACGATAATGGTATTTTTGAAGTCGATCCGTCTTCCTTTGCTATCTTTGAGTTGTCCTTCATCGAGGATCTGCAAGAGGATATTCAGCACGTCGGGGGATGCTTTTTCGATCTCGTCAAGCAAGATCACGGAATAGGGTTTTCTCCTCACGGCTTCCGTAAGTCCCCCTCCTTCGTCGTAGCCTATGTAGCCGGCAGGCGAACCGATCAATTTAGAGACGGAGAATTTTTCCATAAATTCTGACATATCGAATCTAATCAATGCGGTTTCATCACCGAAGTAATGTTTAGCGATGAGTTTTGCAAGGTAGGTTTTCCCTACGCCTGATGGTCCAAGGAAAAGGAAGGATCAGATTGGTTTATTTTTTCTGATCACGGAAAGTCTACTTCTAATCAAGGTCTTGACTACGGCATTCACTGCGTCGTCCTGACCGATAATTTGTCCTTTCAACTCGGCATCGAGTCTCTTGAGTTTGCTTACCTCGTCTTCGTTGACGATATTGGTAGGGATTCCTGTTTTGTCGGCGAGGACGTTTCAGATATCGCTGGATTCGATCACGCTTCTCAGATGTGCAGGAATGTTTTTATTGGATCTGAGTTTCTGGAGGTTTTTTTTCAATTCCTCCTCCTCGGTCTTGAGTTCCGCAGCAGCAAAATAATCCTGATTTTCAATCGCTTTCTCGATTTGTTTCTGGATTTTTTCTATTTTGGTTTCTTGTTTCTTGTATTCATCGTCGTTATCAAGCTTTTTTTGCATGGTGGATTTTCTGGCACTGGCTTCATCGAGAATATCAAGTGCCTTATCAGGAAGCTGTTTATTCAGGATATATCTTTTGCTGAGCATAATCGCACTTTCAATAGCTTCCTCAGAAATTACCACGCCATGGAAATCTTCATAGGTAGGTTTCAATCCGAAGATGATTTGTTTGGTCATTTCGATGCTTGGCTCGTTCACTATGACTTCCTGGAATCTTCTCTTGAGGGCGGCGTCTTTTTCGATGTATTTTTGGTATTCATCGAAGGTTGTTGCACCAATGAGTTTGATTTTGCCTCTAGAAAGTAAGGGCTTGAGCATTTGGGCGGCGTCGTTTTGGTCCTGGCCTCCTGCACCGATGATTGTGTGCAGCTCATCAATAAAGAGGATGATATTATTGGTTGGGTCGGTCGCTTCTTCGAGAATAGATTTCATCCTCGATTCAAACTCTCCTCTATATTTGGTCCCTGCAACGAGGGTTCACATATCTAAGAGAAAAACTTTTTTACCAATGAGTTTCTCAGGCACCTGTTTCGCGTTGATTCTCTGCACGAGCCCTTCAACGATTGCGGTTTTCCCGACTCCTGCCTCACCGATCAAGAGTGGGTTATTTTTGGTCTTTCTCAAAAGGGTATAGATCACTTGGTCGATCTCTTTTTCTCTACCGATGATCGGGTCGAGGTAGCCGTCTTTTGCTTCTTTCGTCAGGTCGGTCCCGAAGTATTCTATCGTTAGCTTTTTTTCCTCTTTCTTTTTGGGTTTTTGATCGCTGGTCTCCAAGGAGAGTTTGTCAGAATCTGACTTTTCTACGATCTCGCTTTCCATAGAATCGAGGATTTCATCGTCTTTTTTGGTGTGTTCATACTGGATATTCATTTTTTGTATATCAGAATAATCTAAATTAAGTTCAGCAATAAGGGTATCTATTATTTCCAGAAATGCAAGGAATCCCTCACTTCTTATCACAGGAAGATTTGATAAAAAGGAAAGTGTGGTTTTTATTTCTCAGAGGTTATTCTTGTCCTTTTGGATCAGCTTTTTGATTTTTGGGCTGAGTCTCTCAAATGCAAGGATAAAGAGCTGGAAAGGATCAATGTTTTTTTGTCCCTTTTCAATTTTGCTCAAGCAATCGGAAGCAAGGATTTTATTGAAGGGGAGGAGCTTCGTCTCGGACTGAAGCATTGGGACTTGAATCTCCTTGAGAAAAATGGATTCCAAGGCTTTTGTCTTTTTGAGTCCGATGACTGCACAGAAAAGGGGAAAGTGGCGGGACTGTTTCAAAAAAGCATAAATGCCCCAGAAAAGCGTATCTGGGGTAATGCGGGTTTGATGGAGTGCGTGGCTGATTCTGCTCGCGTTGTCCAAGCAAGTGATCGCCTGCGTGGTATAAGATTCAAGTGTTTGCTTCATATATCTAAGTGTCTGATTTTCTCATAAAAGGCGTATTCGCAGTCTATCGTTTTGTGAAGTGAATGCAAGAGAAAAAGTCGGAGAAGGAGTCGCGGAGTTTGGATATTTTGCTAAGATTTGTGATGTTTGCGAGAGTAAATTGGATTGATTCGTCCCTGATCAGCTCTTCCCTCAACAAACGGATCGAAGAAAAGAGCGATATGCTTGATAAAGATTCTTTTTTCTCTGTAGGTAATCCCTCTGTCGATTAAAGGCTTGGAAATGATTCAAGTAGGCTTTTCGTCTTCTTAGTGAGCGAGGTAGAAAACTTACGCTTTTTGAGCTTAAAAATCGTTGGTTTTTCTTCATTTATATAAAGCCTCCTCTCAAGCATCTTCTTCGTCCGCTGATGGATTTTGAGAATGTGAGGGGATTTTTATGTGCGCTTATTCGTAGATTTCATCGAGGTGGAAAATGAGGTAGCTCAGTGTAATGCCAGCTCCGATTCCTAGCACGATGCTTATGCTTTGCATTTTGCTAATGAGTAAGAGGTTATACGTCGCATGAATCACAAATCCGACTGTTAGTGCGAGGAGGTATTTTGTTAGGGATCAGATTTTTCTACTTTTCATAATGATGAGTGCGATGGATCCTGTGGCTACGAGATGGATTATGGCGGTTATGAGTCCTCTTCCTAAGAGAATGCCTGTGGTAATGTGCTCTCCCTGTGTAAGCAAAGTGCCGAGTGCAAAGAGATTTTCGATTACACTGAAAGAAAAGCTTGCAAGTAATGAGAAGAGTAAGAGGTTTGAGGGAGATTGGAGGAGAGAGGCTTCGGATTGGTTGTGGCTGAGGGTGAATTTGAAGTTTTCCTCGGTAGCGGCTGCGAGTATGTAGTAGAGTGCGATATTCGTGATATGGAGCCGATCTCTGCTGAGTGAGAATCCTCAGAGGAGGAGGATTGCACTTATGATTTGGAGTCAGATTTTTTTGTTTTTTGACTGTCCAAAGAGGAGGGCAATCCCTATTACTATAAGGAAACTGTAGATAAGAAAGAGGAGGATTGAGGGTAGCGTTAGGCTTTCGTGTGAAAAGATGAAGCTGGCATTGAATCCGAGGAGCGTTAGTGCTTTTTCATAGATAAAAAGTAGAAAAACCAGAATCCCACATTGGAGGAGAGATTTTCGTAGTTTGTAGCCTGATTTCGAAGCACGAGAAAAAACTTGGTTTAAGAACCAAGCCCAGAACCAAATAATTATTCCTATGCTAAGAATGAGACTAATGATTTGCACCTTATTGGGTAGAGGTAAAAGTAGTCCAAAGCTGTTCTAAGTCAGACTTGTCTATTCAAGATGTTTTTTCTGTGATGATGAAGAGCTCTTGACTCGTAGCTCCTTTGGACATTTTTGCTTTTCGTCAGGTCGGTGTCTGGGTTGAGATGGAAAACCTTAGTGCAGAAGATCCTTTTTGCTGTCTTGCAATTCTTCAGGGAATCATTCTCTGGATTCCCGGCTCTTGTTCTAACTTCGTTAAAAAAGGAAGAAATTCAGACAGAATGGTGTGTTCAGTTTTTACTTTTTTGAACGATTCTTTCATCGGATTCCCTACTGATAAAGCTCTTCCTTGATAGCTATTTTGCTGGGAAAAACAAGTTTATTTTTTAGCGAAAAACTTATCATAAACTCCTTCTTCGTATCGTTTTTTCTGGAGCTCCATAAAGTCTTTTTCTTCAGTTCTATTCTTTTCGAGCTTTTGGTCTGGAAAGCTTTGCCCGACAAATCCTTCAGGATAGTTCTTATCGTATTGTAGGTAGGTTGGATCAAGAATAGTTTCTTCTCCTCCTTCCTTTTTTTCTACTACGAAGCGATGTCCATCTCCTTTTTCGGTGTAAGAATATTGTAGTCTTACTTCTTTTCCTTCCGCTTTTAGAGCATTCAAAAGAGCTTTGCTTGCGATATTGCAATAGTCATTAGGAAATCAAGGCTGTGTAGAAAGTTTTTTAATAATGCTGTGCATAATTTCTTCTGAATTGTTTTTTTTGTTGAGGTTCATTTTTTGGAGTGAAGAAATAAAGTTCTTATTCCTATTATATTGCACTTTAGATGTTTTTGCAAATTTTGTCCTTGTAAAAAAGCTTCTCTTTCGTATAGATCAGTATTGTTTATTTTTTTTGAAGGTATGCAGATTCAATCATTTATTCAGGATAGTGCTTTTAAGAAAAAATTTGTGCTGGAGAAACTTCTTTGTCATTATCTCAATAAGACAAGATCAGAGCTGCGGACTGATGGGAATCAGGAAATTCCTCCTACTATAGAGCAGCAAATTAGGGCTGATTATCGTGCGTATGAGGAAGATGATAAACCGCTTGAGTATTTACTCGGGTTCGTGGAATTTTTTTGAATAAAGTTTTTTGTCAATGAAGATACTTTGGTCCCTAGACCAGAGACGGAATATATGATCAGTGCTATTTCTGAGTATCTCTATGAAAAGTCTCAAACAGATATGGTATTGCTTGATATTTGAGTAGGGTGTGGAGTGCTTGGATGTGCGGTTTTGCTCCAGAATGAGGGGAAATTTTGTGATGTTTTTTTCTCAGATATTTCCTCAGAAGCACTTACTGTTGCCAAAAAAAATTATGACACGCTAATCGGTGATCCGTCTTATAACCTTAGGATTCTTCAGTCAGATTTGTGTGCTTTTTTAGAAGACTATCAGGAGATTATCAGAGATAAGGATCTTGTTTTAACTGCGAATTTGCCGTATATCCCTGACAAAACCTTTGAAGATAACGCTCCTCGTAATGTGCAAAAGTGGGAGCCTAAGGTTGCTTTTGTGGGTGGAGAAGATGGATTGGACTATTATAGGAGGATGTTTGAGCAACTTCAAATCCTTGCAAAACCTGCGAAAACTTTGGTGATGTTTCTTGAAATGATGACTTGGCAATTGGAAATTTTAAGGGCAGAATTCTGATCGATATTAGAGTTTGAAGAAGTGAAAACTTTCCATTTTAATATCAGGATTGTGAAAGCGTCCTTTAAATAATGTCTGATTTTTTATTTTTGTAGGTCATTAGCTTATTATGAAGCAGGTTGTATTTATTCACGGGTGAAACTTGATAAAAGATAATGATGAACTTCTAAAAGTCATGCAAACTTGGGAAGTAAATCCTTTTGAAGAAAAAAAGAGACGAAGACTGGTTTTACAGGAGAGGTTGCCTGAATTTTTCGTTATAAAACCTGAAATGCCAAATAAAGATATGGCACGTTATAGCGCTTGGAAGCTTCGGTTTGAGAAGTATTTTCAGTTTTTGGATGATGAAGAGTTGATTTTGATTGGGCATTCGCTTGGAGCGATGTTTTTGGTGAAGTATTTTGCTGAAAATCAGTTTCCAAAAAAGGTAGCTCAGTTGCATTTGGTAGCTCCTGTGCTGGATGCTGAGGGATTACCAGAGGGGGATAATTATTTATGAGATTTCGCCTATGATTTTGCTGCAGTGAAAAATATCTCATCAAAAGCAGAGAAAGTCGTGATTTGGAGTTCAAAGGATGATCCAACTGTTCCTTATGAGCATAGTGTGCGTATTCAGTCGCAGATTGTTGGTTCTCAGCTGAGAATGTTTGAAGATAAGGGACATTTTAATCAGGCGGAGTTCCCTGAGTTGATCCAAGAAATTAGAAAAAAATAGGGGGAAAGCCAGATTTTTTTTCTCTTTGCTTAGAAGAGTCTATAGTAATTATGCTATAGATTTTTTTGTTGTTCAATAACGTTGTATTTTCTTCGGATAGGTTTTTATCTAGAAATATTTACTTTTTTTATAACAAAAATATGGTATGTCAATATCTAGAAACTTGTTCTTCTTGTGGTCGTCTTTTAAAAGGGCACTTGGTAAGCCTGATTTCCTTTTGTTTCCCTAAAAGTAAAAAAAGGGTGAAAAACTGTTAAATTCTCTTGAAAAAGTGAGAAACTCCAGTATTATATCGCTGTACAAAGTACTTTTTATATCTTATTTTGTTACAACAATGACAAAGACAGCACTTATTGCTAGTTTGGCAAACGAACTTGGAGTATCTAAGAAGCTTGCTGCTGAATTAGTAAATGCTTTTATTGAAACTGTGATTACTGGTGTTAAGAAACAGGGAGAAGTTAGAGTTCAAGGATTTGGAACTTTTAAGGCTTCTAAGAGAAAGGCTAGAGAAGGAGTTAATCCTCAGAATCCTAAGCAGAAGATTAAGATCCCTGCAATGAAGGTTGTTACTTTCAAGGCTGGTTCTGACTTCAAAGCTGCTGTGAAATAATTTTTGAAAAAGAATTTATCAAGAAAGCCTGAGATATCTCAGGCTTTTTTTGAATTAATTATTGTTTGGGTTACTGTGAATTTTAGAGGTGGATACTTTTATGCATGTCCTTTTTTTAGTAACTTTTTGCATAAATTACCCTTCTTGCACTTGGTTTCCCACTCACAGGATCAATTCAAGCTTCCTCAGGCATATCAAAGGGCAGACATCTAATCGTTGCTTTAAGTTCCTCTTGCACTTTCATCGCAGTTTCCTTCGTTCCATCCCAATGAGCTAGCACGAAACCTTGTTCTACTTTTTCTTTAAGTTCCTCATAAGTATCTGCAGTAAAGGTATGTTCCAAAGTGAAAAGCTGATGTTTTGCGAGCATTTTTTCTTGAATTTCAAGAAGCAAAGTCTGAATTTCTTCTGCGAGATTTTCAAGCTTCACGAGTTTTTTTTCCATCGTATCTCTCCTAAAAAGTTCTACCTGTCACTGTTCCAAATCTCTCTTCCCAACAGCGATTCTAATTGGCACTCCTTTGAGTTCCCATTCATTGAATTTTCGTCCTGGGGATTTCTGATCGTCATCGTCAATCTTCACTTTGTAAGGGAGATCGTAAGTTCAGAGGAAATCAGACTTTATTTGTAATTTTGAAGCTTTGAGCTGATTCAAAGCAGGTTGCAAAGCACCTAAAATCTGATCCAAATCTTCTGTATTTTTGAAAATAGGAACTACCACGAGATGAAGCGGTGCGAGTGCAGGTGGCAAGACCAATCCATGATCATCAGAATGTGTCATAATCAGACAACCAAGAAGTCTTGTAGTAACTCCCCAAGAAGTCGCATACACATACTCAAGTTCGTTATTTTTATTGGTGAATTTTACATCAAAGGCTTTTGCAAAGTTTTGACCGAGGTTGTGGGAAGTTCCCGCCTGAAGTGCTTTAAAATCCTGCATCATTGGTTCAAAAGTATAGGTCTTAACCGCCCCTGCAAACTTTTCATGTTCAGGTTTCTCTCCTTTATAATGATGCACTGCCATAAAATGAGAAACAAAATCCGAATACACTTCCATCATTTTTCTCGCTTCAATATCAGCTTCTTCAGCCGTAGCATGAGCAGTGTGCCCTTCCTGCCGGAGAAATTCTGCACTCCTGAGGAAAATTCTCGTTCTCATCTCCCATCTCACCACATTTGCCCATTGATTTACAAGGAGTGGTAAATCACGATGTGACTTGATCCAATTTCTATAACTATCTCGGATAATCGTTTCTGAAGTCGGTCTGACAATCAACTCTTCCTCGAGTTTTGCATCAGGATCTACGACAATCCCTTTGCCATTTTCATCATTTTTCAGCCTATAGTGAGTTACTACCGCACATTCTTTGGCAAATCCCTCTACATGATTCGCTTCCTTGCTAAAAAAAGATTTTGGGATAAAGAGTGGGAAATAGGCATTCTGATGCCCGGTTGCCTTAAACATTGCATCAAGTACATCTCTAATATTTTCCCAGATCGCATATCCATAGGGTTTAATTACCATACACCCTCTTACAGAGGAGTTTTCAGCTAAATCTGCGTATTTTACTAAATCATTATATCGTTCAGAGTAGTTCTCAGCTCTTGAAGTCAATTTCGTTGCCATGGTATTCATTTAATCCAAAAAATAAAAAATTTAGAAAATTCCTTTCTTAACCTTTTATTTTCGTGGGGACATGGCACGTATGAATTCACATACTTACACGATATTACTTAAAGATAGAATCAGCATATATTCTGATACGAGTTTAGCGTAATTATCTTATCTTGAAAAGCAAGTCAAAATTTGTTCTTTATGTGTTCTTTGGTTATTGGTTTTCCTCCGTTTATTTCTTTCTCTTGCAAAAAAATGGCAAATCCCTAATCTAAATCAGATTTCTTTTATCTTATTGAAATATATGATGGAAAAACAATTGACTCAATGAAAACAATGTAATTATCAAGTAACCTTTACTTTTACTGATGCAGAAAAGGCTGGTGTGAAGAATCATGTGCTTGAGCATTTTGCAAAAGATATGAATATCCCTGGATTTAGAGCGGGGAAAGTTCCTCTTCATATGGTAGAATCTAAAGTGCAACCTGCCTATGTTCAGATGGCGATTACCGAACACTTGGTAAATAAGGGAATCCAGGAGCTTCTTGGAGAAAATAAAGATTTGAAATTTATTGGTGAACCGTATGCGTTTGATACCAAAGAAGACAAGGGAACTACGACAGTTTCTTTTTCGCTTGATGTGTATCCGGAGGTAGAAGTGAAGGGAAAGTCTTGGGAAAAAGTGCAGATGAATGCCTTAAGTGTTGAGGCAACAGAAAAAGAAGTAGAGGATTCTCTCCTTAATATCCAGAAAAATTATGCTGACTACAAAGATACCGATACGATTGCTGTCAAAGATACCCTTTCCAAGCTCGGACTTGAATTTTTTGATAAGGAAGGAAAGAGTGTAGAAAAAGGAACGACCTATCTCGGAGAAACTGAATTTGCTGAAGATAAGTTCTGGGAGAAGACTTTTGATGGAAAAAAGAAAGGAGAGCTTGTAGAACTCAAATATGATGTGAAAAAACTTCCTGCCGTTTTGCATGCCAAAAAAGGTGATGCTGCCAGTCTAAAAATTACCGTTCAAGATGTGAAGCAAATCGTGCTTCCTGAGCTTAATGATGAAATGATTGTGAAACTTTTTGGAAAAGATGCTGAGGTAAAGACGAATGCTGAACTTAGAGAGTATATCAAGAAAGAAATCTTGAAACAAAAACAGGAAAACTGACTCGTTCAGACGATAGAAGATTATTTGAATGAGGTAAAGAAGGCTGGAGTAAGTGTAATTATCCCAAAGACGATGGTAGATCAAGAACTTAAAGTCAGAATGCAGAATCTTGAAAAGAGATTTGGTTCTGCTGAAAAGGTAAAAGAATATTTCCAGCAGCTTGGAGAAGAAAAGGCGAAAGAATTTGTAGAAGGAGTGCAGAGTGCAGCTGCAGAGAGCCTTGAAAAGTTCTTTATTCTCAATAAAGTTACCGAGCTTCTTGGATTGGAAATTGATTGGAATAGTGAACAGGAACCTTTCTTCGTAGAAAAGCAGCTCTATACTAAATTAGTAGGGGAGTTGGAGACTCCAGCTGATGAGAAGAAAGCAACTAAAAAAGCGCCTGCCAAAAAAACTACTAAAAAAGCTGAATAATATCTTTTAGTTTGAGATAAAAACCTTGAGGATGTGGACTTTCTTCAAAGATTTTCTTGCTTATCCTTTCTGTGCTGATAAGGAACATAATCAGTTTTGAAAGTTTTTGACGACGATTTTCAAGGATTATCCGGCATTCTTTTGGACCAAGCCGACTTTCAAGAAAGCGCTTGCGTTTCCGTTTGTGGTGCTGTATGCGCTCTTTAGGACCAAGAAAAACTAGAAGCAAAAGTCTGATTTTTCATTTCTTTTCTGGGAGGAAGATCAGGCTTTTTTCTATTAAAAAGATTTTCTCTATTTATATTGTATTGAGTTTTTTTAAAATCACTCTTGAAATAAAAGTCAATATAAATATTCTATTCTGGCTTTTATTTTTTGGTAGAGGAATGTTTCACAAGGCTTATGCTTTTGATATTGATGATAATTTGCTTCATACGAATTGCACGGTGTTTGTAGAAAAGTGGGATGGGAGCAAGATGCAGAGGTGCGAAATCCCGGAAAGTGAGCTTAAGGCAAAACTCAAAGAGGGGCGAACTTACCCAAAGAATGATCCGGAAGAGTGTTATTATAATATGAGGCTTGGCGGAGGGAAGCTTACCCAAGATCTTTTTGATGCACTAGATGCGAAAGCTTATGGACCATCGTGGGAGAGGTTTAAACTTGCTAATCTGCAGGCGAGTCCTATAGGAATCATCACTGCTCGTGGACATCCGATTGAGGATCTTAGGAGTGCTCATCAAGCTCTTTTGCATGAGGCGTTTACGCTTGAAGAAAGAGAACAATTTTTGGAAAAGATGAAGCTTCGTTTGGGAGAGCATAGAGCGAGCTCAGATCATCTTATTCGCGATTTTTTTGATATGAACTATTATGCTGCTTGTGCTAATAAAGACTATTCGGATAGTCTTCAGATCCCTTTTGATACTCCTTCTTCAAAGAAGAAAGTTTATGCTTTTGATAAATTTATTGAGCATATCGTGGCGCTCAATCAACACGGAAAGATCAATCCCCATCAAAAAGCTCTAAAAATCTGATTTAGTGATGATAGTTTGGAGAATCTTTCTGCGGTGGAGGAGGCGATTCAAAACACCTTTGTGCTTAGCTATCCGGAAATTACCTTTGCACTCTACAATACCAACAATCCTCGCTTCGTAAAAAAGACGCTCTATGCTTCCGCACAAAATAAAGAACCAGAATTTTAGTTCTTTTGTGAGAGTTTGCTTTCTTAATGTAAGAAGGAAGCTTTTTAACGTATCCCTTGATGATGATCCTCTGAAAATGCTTCTTTTCCTCGTGATGAGGGCTTTTTTCTTTTTTTTGAACGATTCCCCGATTCCCCTCTTTCAAAAGCTTTCAACGTTTCTACGCTAGGAGCTAGGAAACTTCTTCTGGATAAAGTAGTAAACTTAGCAGGCTTTTTTACTGATGGAGCAATGGACGAACTAAGCCACTAGAAAAGAAATCTGGATTGCAACCCTCCCTTCGGTCGCTCGCAAAGACGGGTGGAGCTTGTCGCTTGATTAAAAATGATCTTTGTAAATGAAGCGGACCCCTCCGCTTCAAGAAAAACGACGTTTTGGGACAAGCGGACCCCTCCGCTTCAAGAAAAACGACGTTTTAGGACGAGCGGATCCCTCCGCTTCAAGAAAAATGATGATTTTCGCCAGGAAACTTTTGTAAAATGGGTATTTTTGCATATTTACCGCAGTGGCTATGGTTGCTCTATATTTCTCCTAAAGAACTCGAGAAAAGTTTTTTTTGGTTTGCTAAATATTGCACAAGATTACTCGTATCTTTCTTTTGCATTGAGGCGGGGAGTGTTAGGCTGTGAGTAGTCTTTTTTCTCTGTATGAAAAATCGGAAGATTTTTCTGGTAATTATCAAAAACTTCGATAAAGGCTTGTTGGTTACTAAGATGTAATGTTGGAAGGAATCAGATTTTTCTATTTTTTATTGAGGAATATTTGCTAAAAATGAGGATTGTGGTTATACTTAGGTCGTCTTTTATTTTCTTTTCTTATTTCTATGAAAAAATGGATAAAAAAACGGATGTGGTGGGTTTTGGTTATTCTCCTGCTCGTTCCTATCTTTTTTAAGACGGGTATTATTCTTTATAATAGGATAGATGCTTCATCAGAAAATTGGAAGTACTTCTCTTGTAAGTTGACGGGGAAAGAACAAACCATGATTAACTGTAGCGATAAGAATAAAGATTTTATTATTTCTGGAGATCAAGTGTCGTTTGCAGGGCGTGGATCATGGAATTCAGTCTATATTCCTTTCTCTTCTGACCGCATTACACTTCGTATTGATCCTGAAACGAGAGCTGGGTATGATTTTTGGGTTTTGTATGACACGTTTGGATGGAAGGAGCTTCAGAAGGTTGATAAAGAGCTTATTGCGGATACCACGGCAGAAGAATCTGAGAAGGTTGCTAGATGGAAACGTAATGATAATTTCTTTTTTGAGAAAGATGCGCAAGGGTATGGTGTCATTACAATTGATACGAGAAAACCTTTTTGGCTATCGTTGCAGGGGCTTGGGGCGATTGATCCTTATAAATGGAACCAATTCCTTTCTCAACCAAAGCATCCTAAAGGAGTAATTGTGATGGTTCAAACAACCGGGAAAACCTGACCACAAACGGTGATTACCAGGGAAGAAAGGAATCCTTAGGTTTTTATTCAGGTTTTTTTGGTTTTGGCTTGTATAGTAATGTACGGAGGAAACCTTCCAATCCATTCCGGGGATGGCTAAGGTGAGGGTTTCTATTGTGACCCCTTCCTTCTCTGCCGGTGGTATGGCTATTAGTTCTTACTAGCTATCAAATTAGTTAGTTTCTACTAATTATTAGATTTGTAAACCTCTTCGATAAGCGGAGAGGTTTATTCGTTAATGATGAAGAATCTATGCAAGAGATGATCATAAGAAGTATCGCACTATAAGAGCCAGAGCGAAGTTTCCTAGAGACCATACAAGAGATCGTTTGCAATAGGATTTATTTTTTAATTCCCATACTTGAGATATGACTAAAAAGATGGCAGCAATGCATATTCATACAATAGTATAGGTGTTATAAATTTCTCCTACCAATCAAGAGATAAACATTCCAACACTATTAGCAGATTCCCCAAAGAGAGAACTAATTGTGTTGTTCATGATTAATTTATTGTGAGCATTGAGCGTTAAAATAAATAACAGTGCTCCAAAAAGGGAATAAAACATTCCGAATAACGAAGATAGAATTGTAAATTGAGAGATTCTTTTCGTTTGCATTTTAATATCAGTTAGAAACTAAAAAACGACTATATTATAATTAATATATCATATTTTACAAATTTATTTAAGCAATTGTAAATATGTTTCTTTGTATCATCAATATCATATGAAGTATACATCAGTGTTTCTGTCTTATCTGTTAGTGATCTTTTTTTATAGTATTTGAGGGGAATTTTAATTGCTCTTGTTTTTCAGGCTGAGAATAGTTATAGTTCAGGTTCGTGCTAAAAAAAGAACTTTATTTTCAATTATACTTATGGAGGCAGAAACAAAACAACTCTGAACACAAACTATTGGAAAGCTTTTACTCAAGCAGTCGGCTCCAGCAGTGATTGGGATGCTCGTCCTCGCGTTATATAACTTTGTGGATTCCATCTTTATCGGAAAATATGTCGGAATGGAAGGGCTTGCTGCGACAAGTATCGTATTCCCGATTCAGATGATTATTGGAGCGTTTGCGATGGCGTTCGGTGTGGGAGCGGCGTCTATTATTTCTAGGAGTATCGGTGCAAAAGCATATGAAAAAGTTTGACAAGTATTTGGGACGCTTCAACTGATGACGTTCGGCTTTTTGGTGATTTTGAGTGTGGTTTGACTTTGTTTCGTGCCGCAGCTCTTGGATCTTTTTGGAGCAACGCCTGAGAGTATGGAGTTTGCGAGAGCATATATGGTGCCTCTCTTATTCGGAGCGGTCTTTTTGGGATTTGAGATGTCCAACAACAATATTATTAGGTCGGTCGGGCATGCGACGATGAGTATGAAGATCATGCTTACGAGTGCGATCACCAATTTGATTCTGGATTTTGTCTTTATGAAGATATTTGGCTGGGGAATGGCTGGGGCTGCTTGGGCGACGGTGATTTCCTGGATCGTTTCTTGCGGGATGATGCTGTGGTATTATTTGAGTCCAAAGAATGTGATCCCGATCGCGATCAAGCATCTTCGTATTCATTTGCAGAGTGCAAAAGAAATAATTCTGATTGGACTGCCGAGTTTTTTTAGGCAGATTGTGGCGAGTTTAACGACGGCGTTGATCAATAATTATCTCAGGAAGTATGGAGGGACGGAAGCGATTGCGATTTATGGGATTGTATACAAAGTCTTGCAGCTGTATATGATGCCGATGTTTGGAATCGTGCAGGGAATGCAACCGATTATCGGCTATAATTATGGAGCGAAAAGTCTTGATCGTGTGAAAAAAGTCGTTCTGCTTGGAATTCAGATTTTGACGCTTTTTACGACAGCGATTTCTTTGATTTATCGGGTGTTCCCGACTTTTTTGGTGTCGTTTTTTTCTGAGGATCCACAGCTGATTGCCAATACGGGAAAGGTGCTTCAGATGGTCGTAGCGTGCTTTGCAGTAGTAGGATTTCAGGTGGTTTCCTCTTCTATGTATCAGGCTTTGGGGTTTGTGAAAAAGGCGTTTCTTTTGACCTTACTCAGGCAACTTATCGTTTTTATCCCGGTGCTTTTCTTGATGCTTTGGCTGCGAGAGGATAAACTCCAAGCGGTTTGGCTCAGTTTCCCAATTACTGATGCGATCGGAGTGGTGTTTACGGTGCTGATTTTTGTGCCTGATTTTAAGAAGATGAAGTCGGCGTAGCAGGAGGCTAGCTAGGTTTCTCTTTCTCATCTCGGTGGAATGAGTATGGCGGTGATTGCTCTTGTATTTTTGGTAAAGACTCTTACATTTGGATCTGGTTTTATTCTCTTATTTTTTTAGAGTGATGCAAAAAACAGGAAAGCTGATTGTCATTGATGGAACGGATGGAAGCTGAAAGAAAACGCAAACTGAACTCCTTGTTCAGCGTCTCACATCTGAGGGTTATGAAGTCGAGAAGATTGATTTCCCGCAGTATGGGAAAAAGTCTGCGGGTCCTACTGAAGAGTATTTGAGCGGGAGCTTTGGGACGGCGGCTGAAGTCTCTCCCTATCAGGCTTCGGTGCTGTATGCGGTAGATAGGTTTTCTGCCTCTTTTCAGATCAGGAAATGGCTTGAAGAAGGGAAGATTGTGATCTCTGATCGTTATGTATCTGCGAATATGGGGCATCAGGCAGGAAAGATTGATGATCTCGCAGAAAGGGACCGCTTTTTGGAGTGGTTGGAAGCGTTTGAATACGGGCTTTTGGGGATTCCCCGTCCTGATATTCAGATTTTTCTCTATTTAGATCCAGAAATTAGTAGAAATCTGGCTCTGAAAGTTGAAAAGCCTAATATGGACAAATCAAAAGATATTCATGAAAACGATGCTGAACATATGAGAAAGGCAAGTGAGGCTTTTCGTTATGTGGCAGAGAAGTATGGCTGGACTCAAATTGCTTGTGCTGAGCAGGGTGAGATGAAAACTAGAGAGCAGATTTCCAGTGAACTTTATCAGAAGATTGCTTCGATGCTTGCGTAAAAGATTTTTTGGATAAAGTCTTGCTTTTCTTTTTCAAATCCCTACCCTCAGGAAGTCTTTATATCTTTTCTTTCCCTTTATGTCTCTAGAACAAAATCTGCACAAAAAGATGATGAAAATGATTCTTGGAACTGTTGTTCATATTGAAGATACTGAGCAATCAGAGGCGGTTTTGTCTGCTATGGAAGATACTGCAAAACCTAAAGCTCGTGGTATAAAGCAGTTTTTCTTGTCTGGAATCAACGAACTGAAGAGGGATATTAAGAAAGGAAAGAAGAAATCAGCATAAGTTTTTGTGGTTTGATTATCTTTTATTTTTTTATTGAAAAGTGTATGAAAACAGAAGGATCAAGTTTTTCTTTTTTGGGTGGAGGAGTTGCGTTATTGTGTGCAATGATGCTCCTTTTGGGATGATGTATGTCGCCTCATAGTGTCCAACCCAACGCTAAAGTCAGCTTTTCTTATGTTGCGGTAGATCCAGAGACGAAGGAGACTGTTGCCTCCGGGACGAAGAAAGATATGCTGCTTGACTTTACAGGGACTGCTCTTTATCAGCTTTTGGAGGGAGCAAAGAAGGATGAAGTCAGAACTTGAATTTTGACTGATCCTTTGACTCAGCATGATGATTCGCTTCTTCAGAAGCAAACATCAGTGGTTCTCGCTGAGATGTGATTGCCTAGTGAGGTAGGAACTCGCGTGACTATGGGAGATGCGTCGTTTTTGACAACGGCTGTTGTGACTGAAGATGGGGTAGAGCAGGTTGTTTTGGATGGGAATCCTGTGGAGACTATAGTGCCTTTGAATTGGTCATTTACGATCACTGAGATTAAGTAGTTTTTTTGTCTGGGTCCCCTTTTGTAGGGGAGCCTTTTTTTGAATGAGGATCTTTTTGTCCGGGATTTTATTGAGTTTACTCCTCATTGCATCTTCTGCTTCTTTGGTCTTGCTGGCTTTATCTGTGAACTCCAGAACTTTATTAGAGAGTATTGAAAAGGTCTCTTGTCCGACTATTTTTAGTCTCTGTAGAGGAATTTCATTTTTTGTTGCCCAATCTTTGAGAGAACGAGAAGATTCTTGCAAAGCGGAAGAAGCTCTGTCGGTTGCAGAGATGATAATCTCTATTGAATTTTTCATAGCCATTCCAACTTGCATTCTAAAATAAAACTGTTATAATTCTGTTACTTTATTTACTGGTTAATATTATGTACTGAAATCTTTATAAGGATTTCCCTCAAGCTACAAAGTCATCCGTGATTTATAATCTCATAAACAAAGATCAAAAATCTGATACTCCGCTTTTTTTGTAGTTTTATCTTTGCTCGCTTTGGTGGTAGCGGTAGCACTTCTTATTTTGATACGATAATTATTTCTTACTTTTCATTACCATTTTGACCATAACCGCTTGTATAATTTCTAGTGGACAATTAAGATAATCTTGATAGCTCATTTAAAGAAAGCTCCAAACGAGGAGCATCGAAATTCACAGAGCTGCATAATGATATTGCTCTCCACCTTGTCATTTAAAAAAAGCTCCAAACAAGGAGCATCGAAACAAATCCGAATATATGGTAGCATTACAATACTGAGCTTTAGAGAAAGCTCCAAACAAGGAGCATCGAAACTCGCTAAATCTTCTGCTAGTAATTCTCATGGTTGTCTTTAAATAAAACTCCAAACAAGGAGCATCGAAGCAAACTAAAAAACAGTTCGAAAATAGCCTCCAGAATTGGTTTCGTTGTGCGCATTTTTCGAAAGCTCCAATTGAGGAGCGTCGAAATGTTTTTGATATTTGTTTATTTTTAGTTTTATTAGTAAATAAAAAGAAATTTCTTCTAGGGCAATCTTGAAGTAATTTGACTTGAAAAACTACCATTCTTTTATACTATATTTTCGTTAGTTTTTGTTTTTTTAGTTATGTTTCCTTTGTATGCATCTTTTTAAGAGGCTTTTGCTGACGAAGTTTCCCAATTCAACAATTCATAATGCTGTCAATCCTGCAAGTATGTTTATTGGCTTCATTTCTGCCAAGGAATTTTTTTCGATTATTAATAGGAAGATTATAGAAAATCATAAGCTGAATCTCTTTGATGTGAACTTGGATCCCATAGAATTTAAAATGGGATTTGGAGAGAATCCAAATTCTGATAAACAGAAAGATTTAGAAATTTATTTTGCTTATGCGGTAGCTGCAAATCTCAATGGAACGCTCTTTTTTTCTCCTGCGAATCTAGCAAATGGAGTTTCTTTGCTTGCTACGCTGTATTTTAAGCAAGCGATGACGAGTTTTCCTGAATATTACTTTGTTCAGCTGATTTATACGGTTTTTTTGCTGAGTTTTACCTTTGTATCGAGGGTTAAGGTTTTTCCATCAGAACATGATCAGGAGAATTATAATTGGTTTGTTGAGGTTTTCTTTGATTTTTACAAGATTACTTTTGAACAGTTACAAACTAAGATTTCTGATTCAGATTTTTTTGCTGTTAAAAAGGCAGTTTTACAAGAAACGGCATTCTGCTTTCTCCTTTTCCATTTTTATAAAAAGCTTAATAGTCTGCTTGCTGAGAAATCTTCGGATACTGATTATTTAGATTGGCTTCTTTGAAAAACTAAACAAACGAATTTGATCAAGGCTTTTAAGGAGAACTATGCGACAACAAAATATCTTCCTCATTCTTCTGCACTGGAGCAGAGTATCCTCAATATGGTGCGGCCTGCGGATATTTTGATTAAATATCTTTTTGGTGATGCGAATCCGATTATTGCCGTGGAGACGATCGTTGCAAGGATTTTTGATAAGACAGAGCTTGATCCATTGGTGCAATCCTTCCTTACTTCTGATGAGCAACTTCCTCAGCTTTTTGAGTATCTTTTGGAGTATAAAAAATATAAATACTGATTCTTTGCAGGCGTTCAGAATTATATTATCAAACTTCTCAGATCCGAGGGGAAAGAAGATATTTTGGAGGATATCGATGAAATGCTCTCCGCGATTGACAATGGGGACGATATTTCCAATTTTGATGTGCCTGAGAGGATTAAAAGGGAATCTAAAGTGACGGAGAGGCTTCTTAATTTCTACGTTACTCTGCTCGGTGGCTTTACGACTGCTAGAGGAGACAGCTTTTATTTAAGGCTTTTGAAGCCGGAAATTTTGGATTTTTTTACAAAAAATTCTCTCAATAGTTTGCTGGGGTCGGAGGTTCAGCTTGAGTATTTCGGTGGTGTTTTGTACCAATATGCCAAAAATCTGTACTACTACAGCTATATTAATGAGAATATCAGAGCTGGGAAAAACAAGTTTTCTATGCCACTTAAAGGCGATAGTTCCAAGGTGACGACCAACTTTTCTGTGATTAAACTTTATACTGAGGGGATGATTGCTTCTTTTTTTCAAGATTTAAATCCCAAAGATACCAAACTTACTATTAAAAATACTCAGATTTTAGAACTTTTTAAAACACAGTTTGGAGAACAGGTTTCGGAGATGGTTAAGTTAGGGGCTGATAAATTTTTAGAGGCTTTTTATGCGCCGATTCTTTCACAGATCAAAGATTCAAAGGCTTTTGTGCGTGTTTTATCCTCATCTTTGCAGGAAAATGATCTTGTCAATCTCAAAGATGCACTTTATAAACTAGATTTTTGGATCAGTTTTTCGTTTTTTAAAAAGTTAGAAGCCTTGAAGCTCTGAAAGCAATATGATGATTCCCTTTTGCTTGCGCTTTTTGGAAGTATCAGGGAGACATTCTTTGGATTAGCATTGCTATTTGTTTATCTTGAGCAGAAAGAAAAGGCTGCTTCTCGTGAAAAAAATGAGATTCTTTTGATGGTCTATGTGCGCGATATTTTGGGGATAAAAATTAAAAAAGCTGATCAGGTTTTTAAGGTAATGGTAGAGACGATAGAAGAGCTAAAGCCAATTTTGAAGCTTTGGATTTCTCTGGATGATAATAAAGGTTTCTTTGATCTTATTGCAAAAAATTGGGATTCTTTTTGTGCAGAAAAAACTGAAGATCAACTTCTCGCTTCTTTTTCTGGCGAAGATTTGGTGTGGTTTAGAGGCTTATTAAAGAATATTGCCTATTATAATAAGAGATTTGTGATGCCAAGGTAGCTTTTAGTTTATTGAGAGGATAATGAAAACGGTAGTGATTACCTCGTGATATTTTAATCCGATTCATCCTGGGCATATTGAATGCTTGCAGCTGTGCAAAGAGCTTGGAGATGAGCTTTGGGTAATCGTGAATAATGATGAGCAAGCAAAATTAAAAACCTGAAAATCTGAGGTTTTTCAGGATCAGGAGTTTAGGATGAAAGTTGTTGATTCAATCAAAGGGGTAGATCAGGTTTTTTTGGCTGTTGATGAGGATTGATCAGTTTGTGAGAGCATTAGAGCGATGGCAGAGAAGATTAGGGCTAAGTATTGAGCAGAAACGAAGATTATTTTTGGGAAATGAGGAGATAGATTTGCTGGAAACATCCCGGAAGTGCAAGTATGCAAGGAACTAGGCATTGAAATCAGAGATGGATTATGATCCAAAACCCACAATAGCAGTGATTTTAGGGTGAAGATTTAAAATAATGTTTTAAAAAGCAGGATGCAACAAGTGAAAGTGGTTCAATTTCTTCCCTACTTCCCACCACATAAATGAGGCTTGGAAACGGTAGCAGAGGAGTTTAGTAAATATTTTGTCAGTGAAAAATGCTGAGAAGTTCTCAATGTCACCTTTTCTGTTGGTCAAAAATCGCTTCAGAGTTATGAGCAAGATGGCTATAAAGTGTTCGTCTTGCCTGCGTTTGATTTTATTCATAATTATCCTTTTCCAAAGTTTTGGATTCCGCAGTTCCGATCTGTTTTGAAGCAAGTGAAAAAGCGAAATCCTGATATTATCCAGACTCATACTAGATTTTTTCTTTCTACTTTTCTCTGAGGAATTTGTGCGAAGTTGTGAAAGAAAAAGTGGGTGCATGTAGAACATGGTTCTGGACTAGTGAAGGGATTGGTTCGGTGGAAATCTTGGATTTCAAATGCCTATGATTATACCCTCGGAAAACTGTGTTTCAATAGTGCGGATGAGATCGTAGCTATTAGTAAATGAAATCTTCCTTTTATCAGAAGGTTTTCTAAAACGCCCACTAAAGTGATTTATAGGGGATTGGATTTTCCAGAGATGAGGAGAAAAGAGAAAAAGACTGATCAGGTTTCCCTTGGTTTTGTTGGAAGACTGGTAAAATTGAAGGGAGTGGACCTTTTGATTTCTGCCTTTGCTGACTTAGTCGTTGAAAATCCTCATTTGACCTTGCAAATTGTTGGAGATGGCGAAGAGAGGGGGAGTCTTGAAAAGCGGGTGAAGAACTTAGGATTAGAAGGGAAAGTGCAGTTTTTGGGATTTCAGGATAAGGAGGAAATTCAGCAGAGACTTCTCCCTTCCTGGGATATTTTTGTGAGTCCGAGTCTCCAAGAAGGTTTACCGACGACGGTGATTGAAGCTTTGTTTTCTCAGTGTATTACTGTTGCTACTGATGTCGGTGGAACGAGGGAAATCGCTGATGGAGAGGATTTTATTGTCATTGAGCCTTGAAATGTTGAAGCTTTGAAAAAAGGATTAAATCAGGCTTTAGCTCTTGTTTGAAAAGTGAATTGAGTTTCTTATCAGCAAGTGAGAGAGAGGTTTGACTGGAAGAGAGGAGCCCAGGAGTATTTAGAAATTTATGAGAAATTGGTATGAAAAAAGTAAAATCTGAGACGGATAGAAAATACTATTTCTTGATCAATTCTTTGGAGATAGGAGGTGCTGAGAGGGTCGTTATCAACCAGGCAAATGAGTTAATCAAAAAAGGATTTCAAGTTTATGTTTTTACTTTGAAGTCTGCTTGTTTATACGATTTACCAAAATGAGTCGTTCATATTCCTCTTTCTACGATTAGTGATAATTTTTGTTTATTTTTTGCTTTGCCTCGGTTTATCTATCGTTTTCAGTCTCTTAGGAAGAAATATTGACTAGATGATGGAATTAGTTTCTTGGAAATCTCTAATTTTATTCATATTTTAGCTAGGCGTGAAGCGAAGATTTCTTTTAGAACACATATAAATTTTTTTACAGGTTTTTTTGGATGGCTTCAAAAACTCTTTATAAAAATTCTTTATCCGAGGGCAGCTAAAATTATTGTGAACTCTAGAGAAAATAGACACGATCTTGCTGCTTATTTAGGCATTTCAGAGCAAAAAATTGAAGTTGTCTATAATAGTATTGATGAGGAGAAAATAATGCTGCTTTCTAGAGAGGCTCTTGAGGATGAACTTCAGAAAAAAATTAGAAATAAGCGTGTTTACATTACCGTCGGTAGACTTATCAAAGAGAAACACCATGAAATAATTATGGATGCGTTATCTTGTTTGAAGAATAAAAATTGGATATGGCTTATTGTCTGAGATGGTCCAGAGAGGAGTTTTCTAGAGACAAAAGCTCAGGAATATGGACTGGAGGATAGTATCTTTTTCCTTGGTCAGCAAAAAAATATCTTTAAATATGTTGCAAAATCAGATTTCTTTCTTTATGCTTCCTCTGTAGAAGGATTTCCAAATGTCTTATTGGAGGCAAGATTTTTAGGGATTCCGATTATTACGACTGATTTTAAGAGTGGAGCGAGAGAGGTCATCTTGGGAGAGGATACAAATATGATTTGAAAAGTTCTCAGATATCCCTATAAGGGAAGACACTGATATCTATTAGATGCCAATCATTATTGAGTTCAATTTCTTGAGATAGTGAAATTGATAGGAAAGTAGATGTAAAGTCAGAATTTTTATATTGTTCTTCATTATTGATGAAATTACCTTATTATGCAAAAAGATATATAGAAGGAAATAAAAGTGCTTCTAAATCTTTAGGTTGGAAAGATGAGAAGGTTGATCTTTTGCTGAAAAAATGAGATGTGGTGCTAGATATTTGATGTGCGGTCGGTGGTTTTTATGATAAGCGAAAGCATAAGAAAATTGATTTTCATGGCGTAGATTATAATCCAGATCTGGTAAAATTTTGTAATGAAAAATGATTACAGGTTAAGGAATGAGATATTTCCAAAGGGAAACTGGATTACCCTGATGAAACCTTTGATTTTATTTACTGTTCGCATGTTTTAGAGCATATTTTGAGTAATGAGCAAATTGCGCTCTTTTGAGAGATGTCGAGAGTTTTAAAAAAAGGAGGAAAATTGATGCTTTTCACTCCTACACCTTATACTTGGTATTTCTGGGATGATCCTACTCATCAGAGGCCTTCGACTCATGGTTCGCTAGAACATTTGGCAAAGGATGCTGGTTTGAGAGTGATTGAATCTAAATATTCTAATACGAGATTTTTCCCTCAAAGTGCTCAGAAATGGCTTAGATTGCCTCCATTTAGGTCTTTCCTCTGGGAAGTATATTTAGTAGCTGAGAAGTCTTAAAATGCAGATTAAGCATAATGTTTTGGGGAAGGGAGACAAGAAGATACTCTTTGATATTGAGATGCTACTCTATTATAAACAAGAGTTTGATGAGTTGGTGAAGAGTGGAGAATATACCTTCTATTTTTTGGAAAGGAAGCAGTTTACTTCTCTATTTTCTCACGATCTTTTGAATAGAGTTTCGTTTTCTGGTTTGGATAAAACCTATAAGAAAAATTCTGATTTAAAAGATTATTATCAATCATTATTATCCTTTTGTAGAGAAGAGAAAGTAAAGGCTTGTGTTTTTACAAGTACAGGACAGTATTGGCATCCAGATTTCCTAAATTTATTAAAAAGGGAATGAATAACTATTGCTTTGTCCACCGCGGATGATGACACCAGTGCTATTAAGTATTGTTCGTTGCCTTATACTAAGTATTATGATTACCACTTTCATGTTGGGGTAATGTACGATAAGGAGGGGAAAACGATTGCTGATGTCTTAAGAGAGCATTGATGAAACCCAATATGGGTTCCTTTGTGAGCTAGAGCCGACCATATTAATCAAGATATTCGTTTTGATAATAGAGATATTGAGCTCTGTTATATAGGTAATATGAATCCTCCAAAATTCTTCAGAATTTCAAAGTTAAAAAGGCATTTTGGTGATAGATTAAAGCTCTATTGAGCACAAGGAAATGGGGATCGGAAATCATTGAAAGGAATTTTTTATAAGATTTGTAACAAGCTCTTTAGACTTTGATATATCCAGAAACTGACTGAAGATGAACTTTTGGAGGTATACAGAAGAGCAAAAATCTGATTTAATCTGCATTTAGTTCCCTATAAATGACCAAGTAATAGTAGAATGTATGAATTAGCGAGCAATGGCGTGATGCAGGTTTGTGATAATAAGCTCTGAATGTCTAGGGTATTTGAAGATGGGAAAGAGATTGTTTGTTATGAAAGTATTGATGAGGCTATTGAGAAGATTGAGTATTACTTGAAAAATGAGAAGGAAAGAATAGAAATAGCAAAGGCAGCTTATCTTAGGTCCAAGAATGAATATGCATACTTACATGTAATGAAAAAAACGCTTGATTATATTTGTGGATAGTTTTTTTGTAGAATTTGTTTAGAGGTCAAACGTGAAACCATTACGAGAAATATTTATTTTTTAAAATTGTTAGAGATGATAAGGTCATTAATTAAGAAACTGTATTGAACAGGAATATGAAAATATCCTCCATTTAAACGAATGTATTATCTTCATGATAGATGGTTTAAAAAAAAGTATAAATGAAATAGACAGTATTTAATTACGATTGGTTGAGTAGATTTAAAGATAATAGCTGATGTCACAAATTCTTTTCTTGCTAAAGAAGTTGCGATTAAATGATATTATGAAGAAGGGACCTCTTCCTATTTTTGTCATAATATAAAAAAATGAAGTGTTTTTCTTGATATTGGTGCGAATATAGGTTACTATTCTTTAATTGCATCAAAGAAAGTTTGATTAGATTGAAGAGTTATTGCATTTGAACCTGTCAAGAAGAATTATGAGTCCTTATTGAAAAATATTGAGATAAATAGCATTAAAAATGTCTCAGTATTTAATTTTTGATTATGAAATAAGGAAGAAACTTCAAAGATTCATTTAATAGACTCAGATTCTGGGAAAAGTTCAATTATGGTTGATTATCAATGAGATATTGAACAAATTCAAATGAAGAAATTTGATGATTTAGACTTAGATTTTGATAAATCAAAAGTACAGTTTATAAAAATCGATGTTGAAGGATATGAATTTGAAGTTTTGAAGTGAATGAGGAACTTACTGCAAGAAAACAGAACATTGGAAATTATTCTCGAATTTTCTCCAGAATATTATGAAGAATTTGGAAAAGACTATACTGTTCAGTTTATAGACTTTATTTATGAACTTTGATTTAATGCATTTGATGTTGATCATGGTTTTGCCCCTATAACCTTGACTGAGGAGAATACCTATCCTCAAAAAAATATTCTCTTAAAAAGAAATAGTTAATTTTCTAAAGCCGATTTTCGTTTTTCTACTATTTTATCACTGGAAAAATCTGTATATCTTTTCCTTCATTGTTCTTTATATGAGTTATAGCATTCTTTGTCATTTAATAATAGTTCCATTGCCGATGAGAAAGCTGTTTTGTCGTTATTCGGAGTTGTTATACCATAGGTATTTTCTCATAGAATGTCTTTAGGTCACTGCGTCTTAGTACTAATAATTGGAAGTCAAGATGCCATTGCTTCAAGGATTGCTAGAGAAAAAGATTCAGACAAACTACTTAAGAGAAAACAATCACTTTTCATTAGAAAAGGGAAGATGTTTTCATGGTTTCCTAAAAGGTAAACATTTGAATTGTCTCAGATTTCTTTTTCTAATTGAGGTCTTAATTCTCCTTCCCCTAGAATAATAAGCTGAGTATCCGGATATTTCTTGTTAAGTTTGATAAAACTCTGGATCATGAGGGATTGATTCTTGATAGGATTGAGACGACCGACACTGATAAATGTAAATTTTTTATTTTCAAAGAGAGCAGTATATTCTGCTGGGAGTGCAGCTTTTTGCTTCTCTTTTATTTCATCTAGATTGAGTCCATTAGGAATTATTTGCACTTTTTGTGCTGGAATTTTGAAATTTTGTGTTAGTTGTTGTCTTTCATAACTGGTAAGTACGGTGATAGTATCTGCAAATCTATACAGATATTTAATGCATAAACGGTATAGTCCCTTGCCATATGCCGCAATACTATGATGGATTGATACGATCATTTTTGAATCATTACCAAAAAGGCTGCTGATGATTGTTGGTATGTTAGCATCTTCCATCAGGGAAATCACAGTATTAATCTTATGTTCTTTGCAAAACTTTTTGATTTTTAAAGCTCTAGTAAATAATTTTTTTATTTTACCAAAAAACGAAGAAGTCTGATCCTCATTTAGGGTATAATAAGTTCATTTGAACGGATATTTTTCTTTGCTATCATAGAAAGTAAGATAAGAAATATCATATCATTCTTTATGGAGTTCAGTCCCAAGAATTGAGGCTACTTTTTCAGCTCCTCATCAAAACTGAAGTGAAGGAATAATGATTACGATATTTTTCATCTTTTACTTTTTAGTCTTATGGAACTAAAAAAATGATTTTATTTTTCTTTTTTACCAAATACTACTATTTTTCTATTCCCAACAAAGTATGGAGAGCTCTTTATACACACATAATCTGATGGAAGAATTTCTAAAAAGTTGTGATTAAAACGATAGTTGTTATTGTTGTGAAGCTCATCAACGATAATCGTTTTTATGGTATTGTCTCTGCATAAGGTGGCATAGTAGCTTTTCAGTTCTTCTGGTGGGACAGCATGGATAAAATTAACTGTTATTAGATAGTCTATTTCATATCCCTTAATCGTATCAAATGATCCTACTGAATAATTGCTAGAGGGATACAGTTTTTTTGCTGCATTGATCACTTCTGGTGCAGTATCAAATCCATATTTATTTGGAGATTTTATCTTTGAGAGAATTTCTCCCAGTCCGCATCAGATTTCAAGGACAGTGCCTTTTCTGCTTTTATCATCACTGTTGACTGTTTCTGCTACATAGGTTGTATAGGGTCTCCATTCAGCTGGCAAAATATGCCATCTATCAAAGTGATATCTTTTATATGCGAGGTAATGTCTAAGGTATCTTGCACTCCCCCTCAGTCGTGTCAAAAAGCTCCCGCATTCTTTACTCTTTCTATAAGGAAAGGTAATTAGGTTTTTGATTTGAATTAGTCAACGATAGAGTTTTTGTTCAGCATAAAATCTATAGAAAAAAACTGACTTCCTTCGTGGAGAGCTATAATCCAAGGATTCAATAGCATTGATACAGTGTTGAGATTCAGCTTTACAGAATGCTTCTATATTATCCATAAAAGCTGGACTATAGCTATTGGGATGCAGACAAATGGTCCAGATTCCGCTTCTTTTTTTTATTGGCTTTCGGAGTTGCTGAGGCAGCCATTTGAGACCTTCTCTTGAAAATGGGGAAAGGGCTATCCCATCTGTGATATATTCAAATTTTAATTCTTTTAGTGCCTTACATGTATTTTCATCAAAACTATGTGCTGGAGCCATTCGCCATTTTATTTCCTTTTTTAAGTGCTTTTCAAGGATTTCTTTCCCCTTTTTTATTTTTTCATATTGTTCTTTGTAGGGGAGTCATGCAAATTCACTATAGTTATTGAGAGCTATGATTCCACTGTTGTGCGTTGTGTAGAGGTGTTGATACCCATGCTGTGCAAAGATTCGTCATTTCTCCTCTAAACTTCTCATTTTCTCCCGAAATTCTGCTGTGTATTCTGCAAGTCATAATTGCCTATCTTGATTATCCGGCACAAGTCCAATGATTGGTTTAATAGCGTATCTATCAAAGATTTTTTCTAACCTCAAAAAATTATCTCGATTCATTCAAGGTGTGACATCATCGATACGAAAGATATAACCAGCTTTCTTTGTTTTTGTATGCATTATCTATCACTATTTCATAAAAGGGAGTGAATCAGATCCTTTCCTTATTTTTTATAAACTTTTATAGTGCTTAAATCCTTGTCCTCTTAACTTGCCCTTAATGTCAAAAAGTACTCAATCTTCCTTGAGAAGGCTTTTAATATCAAGCTGCTCAAATTCCTTATGATTTTGAGCATAAATGATGGCATCGTATTTATTTTCATTAGAAAGTGCAGTAAGTATCTCTGCTGGGGTGAGATTAAGTTCTTCTCTATCATAGTCTCTAAGATATTCATGATAGGGATCATATCCAGCAATGTTAGTTCAGAATTCTTTGAGTTCTCTGATGGTCTCTGCTATTTTACTATTTCTAAAGTCAGGGACATTTTCCTTAAAGGTTAGGCCTAATATCAGAATATTTGCGTTTTGTACTTTGTTGTTTGCCTGGATAAGGAGCTTTATTGCCTGATGAGCAACTCGAACTGGCATATCATCGTTTATTCTTCTTCCTGCAAGGATAACATCAGGATGAAGTCAGACTTTTATTGCTCTTCTAGCTAGATAATAAGGATCTACTCCAATACAGTGACCTCCTACAAGTCCAGGAGTGAACCTTAGAAAATTCCACTTAGTTGCTGCTGCATCCAAGACATCATAGGTATTTATTCACATTTTATCACAAATTTTTGCGATTTCATTGATTACTGCAATATTGATATCTCTTTGGGTATTTTCAATAATTTTTGAGGTTTCAGCAACTTTTATGCTTGGTGCTTTGTGAATTGTCCCTGTTGTAATTGATCAGTACATTTCCGCGAGAATTTCCAGTGTTTTTGGGTCAGAGGCAGATACTACTTTTGCGATTTTATCTACAGTATGCTCTTTGTCTCATGGGTTGATTCTTTCTGGAGAGTATCCTACATGAAAATCTTGAATGTATTTCAGTCCACTATTTTTTTCTAGAAGAGGGATACAGATTTCCTCAGTGCATCCAGGGTCTACAGTCGATTCATATACAACAGTTTGTCCTTTTTCTAGCACTTTACCTATCATTGTTGATGCTTTGATTAGTGGTGTGTAGTCAGGGTTATTGTCATGATCAACTGGAGTTGGTACAGTTACGATCAAGATTTCAGCCTTTTTTAATTCTTCTGGATTGTTGGTATAGTGGATATTAACTTCTGCGATTTTGTTTCAGATTTCTTCTGTTGCATCAAATCCATTTTTCAGCTCAGAGAGTTTTTGCTCAGAAATGTCGAATCCGATGAGATTGTTCCCTTTTTTTGCGAGATGGTAGGCTAGTGGGAGCCCTACATATCCAAGTCCAATGATTGCAATAGTTTTCATAGTGAATTTATATTAAAAAGATAAATTCTGATTATATCTTATAGTATTCTCTGTATCGTTTGATAAGCTGGTGCACTCCTTCTTGGATAGGAATTTTTGGTTCTCGTCAGAGGAGCTTTTGTGTATGATTTATATCAACTCAGCTTTCGGGAATATCTGCTAAGTCAGCAGGGAGATATTTTTTATTTCCAGTTTTCCCACATTCTTTTTCAATATAAGAGATCATATCGTTTAGTACAACGGTGTTTGTATTCCCAAGATTGAAGATTTCGTAATGATTTTCTGGAATATAATTTAGAGACTTGATCACTCCATCTACTATATCATCAATATAGGTGAAATTTCTAATTGTTTTTCCTTCATTAAATACCTGGATTTCTTTTTCTTCCAAGATTCCTTTTGTAAAGATAAAAAATGCTCCATCAGGTCTTCCTCGAGGTCAATAGACATTAAAAAATCTGAGTCCAACTGTTGGTAACTTGAAATAAAAGCTATAACTGTGAGCGATAAGTTCGTCTGCTTTTTTTGTTGCTCAATAAAGACTTATCGGAGTATCAGTTTGATCCCCAACAGTAAAAGGCATTTTTTTATTTGCTCCATAGATACTAGCGCTAGAAGCATACACAAAATTTTTGATATGATGCTGTTTTGATAGCTCAATAAGGTTATGAAAACCTACAATATTTGTCTGAATATATGCATAAGGATTAATAAGACTATATCTTACTCCAGCTTGTGCAGCAAGATTTAATACTTTGTCTATTCTATTTTCTTCAAAGAGCTTTGTTACAAATGCTAGATCTTCTAGATTGCCTTTGTAGAAACTGAAGTTTTCAAACTTTTCAAGCTCTTGTCTTCTTGCATGTTTTAGGTTTATATCATAGTAATCATTTTCATTGTCAAGTCAAATCACTATTCCCCCCTGTTCTAGGAGTTTTTTTGAGACATGATATCAGATAAATCCACTGGATCAAGTGATGAGAATTGTTTCCATCTCTATATAATGAATTAAAAAAATCTTTTGATTTCGCTATTTTATACTTAAGTAATTTTTTTTAAATTTCTAATTTCTTGTTTTAAGAGGCTATATTTCTTGATGTTCCATAAAAAAATTAGAAGCAAAAATCAGATTCAGATAGTAATAAGATATAAGAGATTATTTCGTCTATTATTCTCTATAACAAATACTAAATGCTTACTATTCTGAATACAAAGTGTTAATATAGAAATAATTAAAACATTTGATCATATAAATTTCCAATCTGGTTGAAAGTATATTTTTTTCTTTAAGTAAATCAAGCTTAGTCCCCAAGCAAAGAAGTAGCCAACTCAAAATCATACTACAGCTCATAGGAGTCCAAAAAGGTAAATAGAAAGGTTACTTATGAGGATTGTTAATGCTAATGTTCCTACTAGGATGTAAACTCTTTGTCTTACTGCTCAAATTCATCCAAGTATTGCAAAGTTGAAATTTGATAGGATATTAAATATGAGGAAACCGGTTCCCCAGATGCTGATCTCTCATGATAAACGAAATTTTTCTCAAAAAAGAACAGTTGCTATTTCAGGACCGAGGGCTATGAGTAGGGCACTGAAACTTAGTGTAAAAACAGAAAAATAACTATAAAAAAATGAAATAAGGAGATTCAGTTTTTTCTCATCTTTTTTTTCCATAACTTCTGCAACAATCGGAAAAATTAAGCTCATAATCGGTCAAATCAGTACAAATCCTATATTAAACAAACTCTGGAAATTTGCGAAATATCCAGCAGATTCATCTCCCAGAAAATAAAGCACGAGCTGTTGAATAATCTGTCAAAATATGCCACTAATATTTAGTCAAATGAACGACCATAATGCATATTTGCGATATTCTTTAAGCATAGGTTTATTCCACTCAAACTTTCCCTGCATTAAAGAATAATGGTAATTTTTATAGTAAATAAATCCAGCAATGAGTATTCCAACTCCTAATCATAAAAGTCGGTTAAGACTATACCATTCTATTGTCCCTCTGTTGGTCAAAAAACAAAAAAATGAAAATCAGACTACAGATCGCATTCTTATAAATTCCACAAGTTGATAGTCGAAAGTCTTTTGAAATGCAATAAAAATGCTCTGAAATGTCTGAAAAAGATTTATTCATAAAAAATAGAGACAGAAGTATTTTAATATATCAACAGAAATAGGGTTATGAAAATAGTTTATTGCAAGCCAATCACTACCAAACCACAATCCAAAAGCGATCAATATCGCGGTTCCGAACTGGACTAGAAAGCTGAGATAAATTGTCGTTTTGATGTGATTGTATTCCTTTTTGATCCAGAATTTTGGTAAAAAATATTGTAGGCTTTCAGTTAGTCCAAGGTCATTATACACATTTAAAAAGCTAATTAAGCTAATAATGCTGTAGAGAATTCCAACATCCGCAACCGAAACTTCAGGAGAATTAGAAATTAAAAGCCTGATAAGATATCCTCCTGGTGCAATAAGATATCAAAAAAGATAAAGCCAGAAGCCTTTTTTTATTAGTTTTTGACTTAAGGTTTCGTCTTGAAGGAGATCTTTATGTTCTGAAGATTTTTCTGTCATCATTTACAAAAAAACTAAATTCAGATTTACCAACTCCTTGCAAATCTTAAGTCTCCATTCGTAAAAAGCCTAATATCTTTGATTCCATATCTTACGGCAGCAAGTCTGTTGATTCCCATACCAAAGGCAAATCCCGTTCGTCATTCTTCAGGGTTAATTCCAGCTTCTCTGAGCACATTAGGATGGATCATTCCAGCGCCGAGGATTTCCATTCGATTGGATATTTCTTTGGCTCCTGTCTTAGGATTCGTGTATTCATATCTCGTATCAATCTCAAAACCAGGCTCTACGAATGGGAAAAATCCTGGTCTCATTCTGATTTCAACTTCTTTTTGCAGGGCAGCAGAAAGAAACTTAGTAATCACACTTTTGAAATGTCCGATACTGATATTCTTATCAATATAGATTCCCTCAAACTGATAAAACATCGTATCGTGCGTCGCATCAATATTCTCAAAACGATATACTCTTCCTGGCAAAACCGCTTTGATTGGCACTCCATATTTTTTAATCACATAATTCTGACCTGCAGAGGTGTGCGTTCTCAAAACATAATTTTCTCCTCTTTCATCTTTCTCCGCCACATAAATTGTATCATGCATTTCAGTAGCAGGATGAGAAACCGGAATATTTACCGCTTCAAAATTCTCAAACTTGCTCACAATATCCGTTCCACTTTCTACGATAAATCCCATATTCTGAGCGATCTCTTCAAGTTCTCTTCTCACTTTCGTAAGCAAAGAAAAATGTCCTTCCTCTAGTTCAGTTCCAGGAAGCGAAATATCTACCGTTTCTTTTTCTAGTTGAGCATTGATTTCAAGCGTCTTGAGTTCGTTAAATTTTGCTTGATAGGCGTCTTGAAGGCTGGTTCTTATGCTGGAAAGTTTTCCTCCTTGTTCTTTTTTCTCCTCAGGGCTGAGTGTTGCGAGTCCTTTCAAGGCTTCAGTCAGCTTTCCTTTTTTTCCTAAAAGTTCTTGATACCAAGTTTCTAATTGGTCAAGATTATTCATCGCATTGAGCTCCTGAAGGGTCTGTTCTAAATTGAGCATTATCATTCAAAAGTGATAAAAAACTGTTTGGAATTTTAGTGTTTTACTTCCCCTTTGCAAGGAGAATATCTCTTGACATTTTGTGTTTTTTGTATATACTTGTGACTGAAAATAAAAAATAAAAAAATTATGAAAAAGTTGGTTTCTCTTTTAGTGTTTGTTTCTTTCCTTTTTAGTTGTGATTGTGATCGTGTTCTGGATGATCCGGATTTGGCTTGGTCTATTGGGATTGTAAATAATGCAATTCTCAAAGAGCGGACCGTGGCTTTAGAATTTAGGTCTTCGAGACAGGTTCAGAGAGTTGAATTTGAATCATTTTGGAAATTTGAGGTGAATGGATCGATTCTTTCGGTTCGTTTGCTGGATTCAGATTTTACTTGGGAGCAACGAGGGAATGTCCTTCGAGGTGTATGTAAGATTCCTCTTGGGAGCTTGCTTTCTAATCCTCAGGTTCGGCATCGTTATACTCACTTTGTCGGTTATGCGGTGATTGATGGTCGCAGGGTCAGGTTGAGGAATTAGGAAGTGAAAATGAGAAAAAAAGAAGGCTGAGTCTCGTTTTGAGATTCAGCTTTTTCCTTTTAATTTATTTTACTTTTTATGATCTTTTCTCCTTGTTTTCCTAGGGCGAAGCCTTTTCATTGTCGTTTTAAAATGAGAAACTCTCATGGTTTTCAGAACTGTTCTCTAAGGAGGTTATTTTCTGAAAGTTCTTGAGCCTGTTGATTGGAAAGGTCTTGAGTATTTTTCTTGGCAGAGCTTCAAAAGCAGGTTGCAATGCTTTGCTGAGGAAGTCGTTCTCCAGAATAACTGATTTTTATGATAGGAAGTCAGATTTTTTCGGTATGAATTTTTTTGGAGAGGTTTGAGTAGTCTTTGGTAGTGCAATAAATTGCGTTATTTGTCGAGATAAAGGTTGTATTTGCTTGCTTGCAGATGCCTCGATGATGATCAAGATAGTCTCGGACTTTTTTTTGAAGTTCAGGTCAGGTATAAAGTTGAGATTCTTTTAGAGTCCTTTTATCTTGCTTATTTTCTGTCGCTATAGAGGCGACTTTTTTTAGCTTTAAGATGAAAAAGCCTCAAGTGTGTTGGATATGAGGTCGAAATCTGGCTACTTTTTGTGTGTCGTCAGTAGAAAGGAAGGTTTTATCTTGAAATTTGCTGATTCCATTGCTTTTTTGCTCAATAGGAACCGAAATTATTTCTATTGCAGGTCAAATCTTATTGATTACTTGGCTGATCACGCCTTCGTTTTCAAAAGGATTGAGGGTACAGGTCGAATAAACGAGTGTTCATCAGACTTTTAGAGCTTTTATTCCAGAGATTAGAAGCTGGGTTTGAAGCTTCGCTAATTTACCTGCTGCTTTCTGATCTCGCATTTGGACTTTTTTATCGTGCTTATATTGCATCCCTTCCCCGGAACAAGGGGCGTCTACGAGAACTTTATCAAAAGTTTCAGGTGCGAGGTCTCCGATATCTTCTCCTTTATAGGCAGAAATCGCTGTATTAAGCATTCCACAGCGGTTGATGTTGAAGATTAGGGCTTTCCTTCTAGAATTTGAAGGCTCATTGGCTAAAACAAATCCTCCTCAACTGTTTAAAAGCTTGTCTGCAAGCTGGATCGTTTTCCCTCATGGAGAGGCACAGAGGTCAAGTACCAGGTCTCATTTTTCAGGATTGAGAACTTGGGCTGAGAGCCCTGCAGCGACTTCTTGGATGTAAAAAAAGCCTCATTGATGCAGGAAATGTGTGCCGAGTGTTTTGGTATGATAGTTGTGGACGTAGAAAACATCATCATATTTCTTGTCTCAATGTGAGAGATCTGGATATTCAAGTTGCCGTCCTTGATCAATAAAATAGGGGAGTAGATCAGCTTTTTTTGCTTTTGAATTGATGAGTTTGATGCTCTTGGGAATGGTTTGTTGGTAACACGCTTTAAATGCGGAAAACTCGTCCTTATTAGTAAGGAACTGAGCAATAAAGTCGAGATAACTGGTCGTTTTCATGATATAGAGAGGGATAGAAGAAAAAAACTGGTTGTTATGTGCGATTCTTATTTTTCTGCATCCCTTATGAGTGCTTGTTTTTCGATTTCGAGCTGATTGATAGATTTTTCTAGTTGAAGAATTTCTTCTGGCATAGTAGCAAGGTTCATTTTTACGCTGGCTGCGGCCTCATCTAATAAATCTATGGCTTTGTCTGGGAGTTTGCGATCTGGAATATATCTCCTTGATAGGTCGACTGCTGAAACTACGGCATCATCAGTAATTTTTACTCCATGGTGGGTTTCATAGGCTTTTTTAATACCGCGAAGAATTGCTAGAGCATCTTCTCTAGTTGGTTCATTTACCATGACTGGTTGGAATCTTCTCTCAAGTGCGGCATCTTTTTCAATGTAGAGACGATATTCATTGAGGGTTGTTGCTCCGATTACTCTGATTTGTCCTCTGGCGAGGGCTGGTTTGAGCATATTTCCCATATCCATCGATCATTCTGTCTTTCCTGCTCCAACAACGGTATGTAATTCATCGATAAAAAGGATGATTTCTCAGTCTGATTTTTCAACCTCTTTTAAAATGGCTTTTAGTCTTTCTTCAAAGTCTCCTCTATATTTACTTCCTGCCATAAGTGCTCCCATATCGAGTTCAATGATTTTTTTGTTCTTAAGAAGCTCTGGAACCTCTCATTTGACGATTTGTTGTGCAAGAAGCTCAATAATGGCGGTCTTTCCAACTCCAGGATCTCCGACAAGGACGGGATTGTTTTTGGTCCTACGAGAAAGGATTTGGAGTACTCTTCTTAGTTCTTCATCTCTTCCAATGACAGGATCAAGTTTCCCTTTTTCTGCAAGAAGCGTAATATCTTTCCCATATTTAGAAAGAGCTTCAAGGCTGACTTCTGGATCATTTGTTTCGATTTTTTCTCCGTTCCTCATTTGGAGGATTGTTTCGTGGGCTATTTGGTAAGTAATGTTAAGCTTTTCAAGGAGCTGCTTGATCTTGTTGTCGCTTTTTAGAATGGTGAGAAGAAAATGCTCGGTCGTGATATAGGCATCTCAGAGCTCTTTCATTTCTCTTTCGCTTTGTTCCAAGATGCGGTTTAACTCGTTGCTAAGTCCGATTTGGTTTGTCCCTTGAATCCTTGGGAGCCTGTCCAAAAGTACAATGGTTTCAGCTTTTATCTGTTCGATGTTTTTCCCCATTTTTTTTAAGATTAAGGGGAGGTAGCCATCAGTCTGTTCGAGCATCGCAAAGAAAAGATGTTCTGTTGTGATTTCATTATTCCCCCTATTGATGCAGAGGCTGTTTGCTTCTTGAATTGCCTCGCTGGCTTTAATGGTAAGTTTATTGAAGTTCATTTGTTTAATATTATGAAATAAAATAGCACTTATTTTTATCTGGTGCTATATATAGTAGATAAAAAAGATGAAAATGCAAGAGAAAATAATCTATCTTATTACTCAGATAAGCGTCAAGCTAAAGTTTTTTGATTTGGCAGAAAATATCGCTATTCTATCTGTAAATTTTTTGTGCTGGAGGAGCTTAATTATTGAGTTGAGAACTTTTTTGATAATAAAAAAAGAAATCTGAGGTCAGACTTCTTTCTTGTTCTATTTATTTTTTTCAGGATCTGAGGCTGTATCTTGGTTTCTGAGCTTCATAAGTGGGAACATTACCACGTCTCTCAAGTTTTCTTGTTCTGTCAGAATTGCCAAAATTCTCTCAAGTCCCATTCCAAAACCAGACTGTGGAGGCATTCCATACTCCATCGCCTCTACGAAGGTATCATCTCCACTGGTCGCTTCATCATCGCCTTTTTCTGCAGCAGCAGCTTGTTTGTCAAAGTTTGCTTGTTGGAGTAAAGGATCTACAAGTTCAGAATAGGCTTTACAAATCTCCCAACCATTTACGAGGAGCTGGAACTGCTCTACGATATTTGCATTAGCATCAGAAGTCCTCGCTAAAGGCTGCATAATCACTGGATAATTATAGATGAAAGCTGGTCCTACGATCTTTGGCCTGAGCACCTTTTTGAATAAATAATCAATCAAAGTCGGAGTTCCCATCTCGTCCATTCCCTCAAATTCAATGCCTTTTGCACGGATGTCAGCTCTTAATTGATCTGCATCATCAGTATCATATTTGGTAATATCAATCCCTGAAGCATCAAGGATTCCCTTGGTATAATCTATTCTTTCTCGTGGAGTTGTAAAATCAACCGTTTTCTCTATTCAGTCTTTATCTTTTACTTTAAGCTGCTTAGAAATCCCAAGTTCAGCAAAGAGATAATCAAACATTTCCTCAGTAAAACGCATATTATCCTCATAGTTCCAATACACGGCATAGTGCTCCACCTGCGTAAACTCCTGCAAATGTGAAGGATCAGAACCTTCATTTCTGAAATCCTGCCCAATCTCAAACACTTTCTCAAATCTTCCAACCGTCGCCTTTTTGAGTGAGGTTTCAAACGCGATTCTGAGATAAACATCGGTATCATAGTCATTATGATGGGTAATAAACGGTCTTGCTGCCGCACCAGAAGCCGCATTATCCAAAATCGGAGTCTGAATCTCAATAAAGTTATGCTTACGATAAAAATCCCTTAAAGTCTGATAAAACTTAGACTTAAAGAGGAATCTCGCATAGGTTTCAGGATTCATCGTCATATCAAGGTATCTTTTCCTATACAGCTCCTCCTGATCGCTCAAGCCGTGGAATTTTTCAGGCAAAGGTCTAATCGCTTTGGAAAGGAATTTGAAAGCTGACACGAAAATTGTGAGTTCTCCTTTGTGAGTTTTGAAGACTTCTCCTTCTACTCCGATAAAATCTCCGAGATCCACCATTTTTTCCATAAATTTGTAAGCCGTCATCGTTCAGGTTTCACTCTCTGGAAAGTCTAAACTCTGAACCTCAATATCTTCCATGCTTCCATCAGCTTTTTTTTCTGTTTTGATAATCTTGCACTGATCTCTATGAAACATCAGCTGAATTTGCTCGGTGCTATCAAGGATTTTTGCAAAAGCAAGCTTCCCATGCGAACGATAGAGCATCACTCTTCCAGCAGTTTTGACCTGCACTTTTGGAGCTTCGATAATCGCCTCAATCTCGCGAAGCTGCTGATTTTCATAAGTCGCGATAATATCTTTGATGAGGTGCGTTTTGTGAAAAGATTGAGCATAAGGATGAATTCCCATCGCTTGAAGTTTCTGAGTTTTCTGTATTCTGACCTCTCTTTCGCTAAGTTGAACTTGTGCCATTTGCAATTATAAAGGAAAAAGTTAAAATCTAACTCTGACTATAGGAATTCCCGCAAGAAATGCAAGAAATTTCCGTGGGTTTGATGTTTTTCTTTGATGCTTATCTAAGGAAAGTAACGGAGAGAAGCATATTAAGCTCTGATTAAAAAAATTTATCATTCAACTCTTCATATGCTCAATTCAACTTCAGAGAAAAAGCAATTCTTTCAAAGTCTCATTAGACGAGTAATGATTTTAATTGGGATCAAAGTTCTTATTATCAGCTTTTTCTTTTTTCACTTTCTAGAAAAGAATAAACTCAGTCCTCAAAAACCTCAAATGTGTTATGAAAATCACTGTTTTGAGCTTGAAATTGCTGATACACCAGAAAAGAGAGAACTCTGACTGATGTTTCGTGAATCACTTCCAGAAAATCAGGGGATGCTCTTTGTTTTTGATAGATCAGACTTTTGGAGTTTTTGGATGAGAAATACCTTGATTCCGCTTGATATTGTCCGATTAGATACGGGGAATAGAGTTGTGGATTTCGTTTCTGCAGCTCCGTGTGAGAAGATGCCGTGTCAGAATTATTTTCCGAGGTCTGAAGCGATCCAAGCGATCGAACTTAACAGCGGCACATTGGAGAAACTGGGGATTCAAATTGGTCAGGAGCTTAAGATTATTAAATAGGAAAAAAACAGGGAAATTTGCTTTGTTATTTATAGTCTTCTGAGTAAAAAGTTATAGCTTTGTTCCAGACGTTTTGTGGTAAATGTCAAGAGAGGATGGATTTTGGGGAGAATTGAGGATTGCTCTTGATTTTCAGGGAAAAATGCCCATACTTTAGTCAGATTTTTTATCTTTTATTTATTAGATCATGACTCACGAATACTTTATGGAGAAGGCGGTAGAGTTGTCCCTAGAAAATATGAGAGCAGGGAAAGGTGGACCCTTTGGTGCGATCATTGTGAGGCAAGGGAAGATTGTTGGAACGGGGGCTAATCATGTCACTTCTGACAATGACCCTACGGCACATGCTGAGGTGGTAGCGATCAGAGATGCTTGCAAGAACTTGGGGACGTTTCAGCTAGATGATTGTGAGATTTACACAAGTTGTGAACCTTGTCCGATGTGTCTTGCAGCGATTTATTGGGCGAGACCAAAGGCGATTTACTATGCCAATACGAGAAAAGATGCTGCAGATATCGGGTTTGATGATGACTTCCTTTATGAAGAGCTCAAAAAGAACCTCGAAGAAAGAAGTCTTCCTATCCATCAGCTTGATAAAAGCAATGCACTCAAGGTTTTTGATGAATGGAAGAGAAAAACTGACAAGATTGAGTACTAAAATGTTGCGTTATTTCTTTTTTCTGTAGATTCAAGGTCAGATTTCTTGGATTTGCTTATCTATTTCTAAGAAGGTTAGCTCTTGGAGGAGTTCTCCGTAGCTGAGATGTGTGTAAGCAAGCAGACTTTCTAAGCTGGAATCCCCCTGTTTTTCGAGATGTTGTAGGAGCTCGATTTGACGGTCGGTTAGTGATGTTTTTTCTATTTGCAATACTGCTCCTTTTTGAGGAAAGTGTTTTTGTAAGCAGGCGTCAAAGTCTGTGATGAGTTGGATTTTTTGTTCGTTCATTTTGGCAAAGACGCCGGCACTTTGAGGTGAAAAGATAGGAGCTGGGACTGAAAATACAGGTTTTCCCATAGTGTAGGCGAACTCTGCGGTAATCAGACTTCAAGACTTTTCTCTTGCTTCGGGTAAAAAGAGGATTTCGCTGAGTTTTGCAATGAGTTTGTTTCTCTGGGGGAAGCTTCGTTTGGTTGGCTGGAATCAGAGTTTAAATTCTGAGAGAATGAGTCCTCATTGATTTATGATTTCTGCGAGAAATCAGCGATCGGAACTTCTGAGATAGTGTTGGAAACCTCATCCGAGCACTGCGATGGTTGGGATGTTGTGCAGTAGTGAGAGTTGATGTGTTTTTTGATACACTCCTTTTGCAAATCCTGAGAGCGAAGCAAGCTGAAAGTTGCTTGCTTTTTCAAAAAAAAGTTCCAAAACCTGAATTCCATATTCACTGGGCTGTCTTGGTCCTACTATTCCGAGAATTTTTTCCTCGAGGAGAGAAAGATTCCCTTGGTAGTAGAGCAAAAAAGGAGCTTGGGAGTCGTTCATCAGTCTCTGAGGATATGCATTATCTCATTGCACGATGCATCAAATTTGGTTTTTTTCGCAATACGCTTGGATTTTTGAGGTTTCAATGTCAGAGTTGAAGTTTTTTTTCTTGAGATTTCAGAGAGTTAGTGCTTCTTGGGCAGAGTAATGGAGACCTAGTGGATTGAAATACATTAAATTCTGATATGGTTAAAAGAATGACACTGAGTATAAAAAATGTTTTTTGCTTTTCAAGTGATTATTTGGTTTTTTTCTTTTGTCAAAAAAAGTTTTGATGACTTTTTGTCTTGATTTTTGGTTATGAAATCACTATTATCAATGTTGTCAATAATGACATAATAACAAAAAAGATAAATAATATGAACTGGAGAAAAGAAAAAAGAAGCCTTCTTAATCAGGTGAGGCTCAGTGTACAACGTGAGATAAGGTTTTCAGGGAAAACGGCGAAGCAGCGTATCAAGTTTTTACAGAACTTGAGTCGTAGTTCTTGTGACTTGGAAAATCCAACATTTGGTAAAGTTAGACCGGGTGCATTGGTGGCTCTCAAAACCGCCGAAACTGTGACTTGGCTCTTTATTCTGCCGTTTGGATTTGATGCTTCTTACAAGCTACGAAATGAAACTCTCAAAGAGGTTGAAGTCATTTCAATCTTTGATGAGCGGTTTAAATTTCTGTGTGAGAGGTCTTCCGGAGCCTTGGTAGAGATTGGAGAAATTGTCCAAGTTCACTGATGGAGTAATCATGTAAAACCACTTTGTGTATTAAAACCACTTTGTGTATTCGCAGAGTGGTTTTTTAAAATAATCCTCCTTGGATAATCCTTTCATTTTCATCTTTTAGCTTTATTGGAATTGTGAGATGGAATTCTTTTAGTATGGTTTCTCGATTTTCATAGATTAAAATTCAGAATTTTCTTGCTTTTTCAGCTTTGGATCAAGGTTTTTCTCAGATAAGCATAACGGTTGTCCCTGAAGTCGGATTTTCTTCGTAGCGATAGCCTAGTTTTTGCATTTGCTGAATGAGGTCGGTTCTTGCAATGGGGAAAGTTCCCGTGAGCGAAAAGGTTCAGTGTGATGAGGATTCAGGATTTGTTTTTTCATTTATTGCGGAGAATTGTACGCCTTCTTGTTCTAAGCTACGCAGAAGCTTTAAGGTTTCGGGATTCGTCATAAAAAGCTGAATTGCTTGTAGCGATTTTTCCCCAATACCTTCGACGTCTGCTAAGCCATCATTAGAAAACGCACTGATCATCTGATCTAGTGACGTTACTTTGTGTAAGGCAAGATATTCTGCTACATCTTGAGCTGTTTTTTTTCAGATATGAGGGATTCAAAGGGCATTGAGAAGTCTCCAGAGTGGTTGTTTTTTTGAACGTTCAATTTGATTGACAAGCTCGGTGATTTTCTTTTCTCCAAAGCTTGGGAATTTACGCAGGAGAATCTGATTTTGAAGCGTAGTCAGGTGGTAGATATCTGCGACTGAATTCAGCATTTTTTGTTTGACTAAGACCTCAATCATCGCTTCACCAATTCCACTAATATCCATCGCATCTCTCGAAACAAAATGCGTAATCTTTTCTCTTATTTGTGCTGGACAAGAGGGATTGGTGCAATAGTAGTGAATATCTATATTGGTAATCGGATCTCCGCATTCAGGACAGAATAGAGGAGGAATAATCAGCTCTTCACTCCCATTTCTACGTTCTTTGATGCTTCCCAAAATGTAAGGAATCACTTCGCCACTTCTCTGCACTCGGACAAAATCTCCCTTCTTGATCTGCTTTTTGGCAATAAAATCAAAATTATGAAGACTGACCCTAGAGATCGTGGCTCCGCTGAGGGAAATCGGTTCAATATGGGCGACTGGTGTGATAATTCCCGTTCTTCCGACTTGGAAGGTTATCTCTTGGATCTGGCTTGCGACCTGTTGCGCGGGGAATTTGTAGGCGATTGCTCGTCTAGGGTGATGTTCCGTAGATCAGAGTGCTGAACGAATGTCTTCGCTACTATTTACTGGAGTCTCTGTATCAAAAAGAGAAAAGTCAGATTTTTCTGAGGTATTGCAGTTTGACGTATTTTGAGGAGTTTTACTATCAGAAACCTTAATTACCAAGCCATCAAAATCAAAATCTTGCTCCTCCAAAAAGTGCTTGATTTGAAAATCCTGACACCAAGTGATAATTTGCTCAATATCTGTAGTCACAAGGAAGTTTTCTGGCAACTCAAAAACGGGCATTCCAAGCGACTTGAGATCCACTATTTCTCCTCAGAGCTGATCATATATGAAAGCAATCAAGCCTCTTTTCGCAACTTCTCTGCTATCTAGGAGTTTTATGCTTCCAGCAGCGGCATTTCTCGTATTTGAAAAGGGGGTTTGACCGGAGTGGTCTCTTTGCTGATTGAGCTTGAGGAGCTGGGATTTTGGCATCATAATCTCTCCTCTAACCTGCAAAGTTCAAAGATAAGAAATCTGCTTTGGAATTGAAGGAATCATCATCACATTCGCGGTAATGTCTTCCCCAATTTTCCCATCGCCTCTGGTGATTGCTTGGGTGAGTTTCCCATCATGATAAATGCATTCTACGCTGAGTCCATCAAATTTTGGCTCTAAATGATATTCCCGGTTCAGAATTCCTTTTTTTTCTGCCAACTTTGCAACTCTGACTGCCCATTCTCTAATATCCTCTGCATTATAACTATTTTCTAGGGAAAGAAGTGGGATTTGATGGACTGCCTTTTTGAAGCCGTCAGAAAGTTGTCCTACTAGACCTTGCGTTGGAGAGGTGCTGGAAATGATCGTCGGGAAGTATTCTTCCATTGCTTTGAGATAGGCGAAAAGTTCATCATATTCTGCATCAGAGATGATTGGCTTTGCCTCAATATAGTAGAGATGATTATGATCGGTTAAAGTATCGATGAAAAGCTGGTAGATCGTTTTCAATTCTGAATCGGGGATATGTTCAGGATTTGGAACTTTTTTGATGAAAGATTGAAGGTATTGAGTAGATTCTGAGAGATTCACGAGTATTAGGAAAAGAGTAAAGTCTGATCTTAGCTTAGAGAAAAGCAAATGAAAGGCAATATCAAATTATCGGTTTTCTCTCCTATTTTTTCAGATAGCTTATGTTGACCAATTGTCCTTTGTTCTCGGCTAGAGCTATTTTGTCAATGATGAGCGATTGGATGTTAAAGTGTTGCTTGAAGTAGGTGGCGGCGAGATGCATTTTTTCAAGACGGATATAATTGCATAGATTCATATGATTTGCTAAATAGTTAAAAATGTCTTGTGGATCTCGGTATTCTCAGGTTGCATTAGCGATATTTTGGGCATACGCTAAAGAGTCGTCAACATCAAAGTTTTCGGCAATTTTTCTTATTTTTTTGGCGGTGTGGATCAGGTTTTGATTCTTTGGAAAGAAAAGTGCGAATCGTCCACATCATTCAGGATATTTTTTGTTTCGTTCTGGATTTTTTTTGATTCGATCGTGCTCTTGGAGGATTATCTGAAAGTTAGTCAGGTTTATTTTTCTAGTATTGATGAGACTACTTTCAACTTCTGACCAAAAATTTTTAGATGAAGAATCCTTTAACTGTAATAAGGTAATATGTGCGTTTTCTTTGCATTTGGAAACTCTCTGTCTAAAGCCATTTTGGTAAAAATAATTTCTGATCGTATTCAGTTTATGTAATTGATCATCCTGAGGAGGTGCAACTATGGTATAGAACATCGGTTTTGTGTTGGAATAAATTCTAGTCGACTATATCGATATTGTCTGAAGATACAATAAAAAAAACGGTTGATATGCCTAGTATTAATGATTATACTTTCGATAGTTTTTTTAATTTATTGATTGAATATTGTGTTTAAAAAATCAATATATGGCTTTTACTTAGCGACACTTCTTCAAAATGCATTAAAGGTCATTACTCCTTTCTATATTATTTATTTTGTAGCTTTAGGATTTAGCTTTTGGCAAATTGCCTTGATTTCTAGTTTAAGAAGCATTGTTGGACTTATTTTTGAGATTCCAACAGGAATGATTGCTGATATTTATGGGAAGAAAACGAGTGTTATCCTTGGTTATAGTCTTTCTGCTCTCACTTTGTTTCTGATTCCACTTACCGATAATTTTCTTTGGATTGCGGTTGTTTTCTGTTTTAATGCTTTTTTTGAAACTTTTTTTACAGGGGCTGATAATGCTTGGGTTTCTGATAGAATAGAAGCTGAAAATCCTGATGATATGAAGTCATTTTTTATGAGGAAAATGAGTTTAAGAAATATTGGATTTATCGTTGCTGGGCTTTTGTGAGCCTGAATTGTAAAGTATCGAGGCATAGAGTATTTGCGATATGTGTATGGAGGAGGGCTATTACTTGCTAGTGGGGTGCTTTTATTCCTTCCTGATGGGATGAAAGGTTATTGCTGAGAAGAGGAAAATATTCTCAGTAAGATATTTTGGCATCATTTGAGAGACTCTCGAAAATATCTTGCTCAGCATAAAACCTTATGGTTTTTATTTGCAGGAATCGGGCTTTTTCTTATTTTAGATGAATTGACAGGATTGATTTGGGCGCCTTATCTTGAAGAGCTTGGTTTCTCAATAGAGAATCTTGGCTATCTCTCATCACTTTTATGACTCTTATGAGCTGGAGTTCCGCTCTTGCTTGAAAAGGTTCTTAAGAAAAAGAAAAATAGTCCTCTTGTTTTGGGTACTTCACTCTTGATCTTTGTAGGAATTCTTATCTTCGCAGGATTGACTTCCTCAATTACAATGCTCATTTGCCTCTACATTATCTACAATTTTATTGATGATATTATTTTCCCTGTAGATGAGCTATTGACGAATCAGCTTATCTCAGCAGAAAAAAGGGCAACCATTTTATCTATGAAATCAGTTGTTGAAAATTTGAGTTCAATTCTTGGTTGACCTTTGGTAGGGTGGATTTTGTGATATTTAACTTTCTCTCAAGGTCTTTTTCTCTCTGCTGGTTTGCTTTTGGTTATTGCTGGAGTCTATGTGTATTTTTCTTGTTATTATAATTTTTCTATTAAGCATAGGTCTTAATTGGAATTATCTTTTGTTTCTATTTGCGAGAATTGGGACTTTGATCTGTTGGGTTAAGAATGTAGAATTAAGAATATGGAATATAAGAGAAGAGATAAATATCATACTCTCATTTTAGATAAGCATCATTAATTTGACTTTATAAAAAATGCAGTTATAATAAAATTCACAAAATCACCTTAATAGTGAAAACAATACTTTTTACCTATCTACTTGATATCCTCCATGTCTCGCACAAAATCGAATTTTTGAATCCCGAATAAAACTCCAGATAATACTTCCTCAAGTTCATGAAATTGGTATGAAGAATACTCCTGTATTATTGAAAAACCTACATATGAATGAAGACCTAAAGTTACACAAGAGCGTCCTGATGGTTCATCAGATGGGCTCTTTGAAGCACTTGATATAGATTTATGAGATGGGACTAAAGCTTGCTCTAATTGGGAAAAATTTGATAAAAAATGAACATTAGAAGAAATTTTCCCTCCTTTACGTGAACTAACAGCAGAGAAGCTCGTTGAGGAGTTTTATAAAAATCAGGAACATAATAGTAGCAAATAAAAACTCCTAGTATTTCCCTAAATTCTGTAAATTTTCTAACTTCTCCATCAAATCTCCATAGTTTTTAACTGGAATAATCTTTTGCTTTCATTCACGAGAGCATGGAATGCCTTTGATATACTGAATAAATGTTTTTGAAATTTAATGACTCAGTGCTAGTATTGATGAGGATTCAGATTTTTTTGTTTTCTCTGAGGTATTTCCTCATCCACTTTTCAAAAAGTTCAGAAAAAGTCTGGTTGAGTTCTTTGGTCATATTTACCAGATAGATTTTCTTATTTTAGTGATCAAAAAGTTCGCAATTCATGAGCAGAGTCTAATTATTTAGAAAATAACACTTCTCTCTTCTCTTAGAGGAGTTTATTCTTACCAGCATCTATATCTAATACTTCATACTTTTCAAGTAATGTTTCTAAGCTTACTTTATACATGACAGGGACGAGGTTTTCAGAGATGAGTATTGTTTTTTCTTTTCCTCATTTTTTATTTGTTTTTTTGATGGTATTGTATCTCCAGAATGTCCCTTGATAACGAATAAAGCAATGATCTTCTTCAAAAACATGAATTTTCACTTCTTTGGCTTGAGGCGAGCGGATTTGTCCCTTGAGATATTTCTCATCAGTCCAAAGTTTGATTTGAATCGGGTATTCCAAAGTATTTTTTATCTTGAGATCTACATAATTGTAAAAAACTGTTGCACCTGAAGCAAAGGGCAAGACTCTCCCAGAATCTGGAAACAGATCATAACTATGACGGTGCCTTTCTATAATTTCTACTGGGAGATGCAAAAACATCCAGTACAAGAGATTCCCAAGCTGACATAAGCCTCCTCAGATTCCTACTTTTACCTTTCCTTTTGAAATCAAGATCCCCTCTAGATAGCCCTTTTGGGCAGTGGGTTTTCTCACCAATTCCCAAAAAGAAAAAATTTCTCCAGGCTGAATCAGAATTCCATTAATTTTTTCTAAAGCGATTTGAAGATTTTTTACTTTATTGACTTGAAGCTGCATATCCACATCGCCCAGCTTTCTGAGTAAAACGGATTGATGTTTTACATTGATATAAGGTAAAAAATCTGATCTTCCCCTTGCAATTGGCTGAAACACATTTTCAATTCTCCTTATCCATTGTTTTATTCGGACAAAAAATGGCATCACAAGCGGAAACTTCATACTTCGGGAAGTTTTGATCTTTTTCTGCTCTTGAAGCAGTGTTTTGATATGCTCAGCTAATTGAGGGTTATGAAGTAGAGGCATAGTTAAAGAAAGGGTATTAAAGCATATGTTGAAGCTTTTCCAATTTTTCCATCAATTCATCATAGGTCTTGACTGGAATAACTTCTTGCTTCCATTCACGAGAGTTGGGAATGCCTTTGATATACTGGAAAAGATACTTTCTAAATTCAATTACTGAACGATATTCAGCTTCAGAATTGATTTCTTTTTTTAAAGATTCTAAATCTGATTTTTTTGGTTGATTGAACCTATAATCTTTTACTTTTTCTCCTCGGTAATCAAATCGCAACTCACAGGCAATCATCACTTCCAAATGTGCTTTGATCACTTCAATCTTCTCCTCCAAAGTCGGCTCGTGAGGCGTAAAAACCCGAGGGTTCCCAATCGCCCCCTGCCCAACCATCAATGCATCAAAATCTCGCTCTTGACTGATTTTTTCGGCTTGTTGATAATTCGTGATCCCTCCATTCGCGATGATAGGGCAAGTAACCTGGCTTTTGACCTGCTGAATAAACGAAAAATCCGCCTCTCAGCTATAAAGTTGCTTGAGCGTCCTCCCATGGATAGTAATCAGATCACAAAAAGGAGCAATCTGACTAAGAAACTGGAGCTGTTCAGGCTTATCGTCTTCATTCAAACCTGCTCTGGATTTCACCGAAAAGGTCAGCTTTTTAGTGCTTTTTATGATTTCAGAAAGGGATTTAATGATGGCTAGGGTTTCAGTGCGATGCTTGAGCATATCAGATCATCAGCCACATTTCATCACTGTATTGCTCGGACATCAGGTATTCAGCTCAATTCCCGAAAAATCATCAGCATATTTTTCCACAAGAATTTTAGCAGTTTCAATGAGCGTTTTTGCGTTTCCTCAATAGATTTGAGCAATGGGCTTGGTAAGTGTGAAGTGTTTATTATGGATTGTTTTGTCCGCTTCGTTCCCTCGCAATGACGGTCATTGTCAAACATCATGAACAGTCATTAGATGCTTCACAACCTTACTCGGATTGATAATGAAACCATCAGCATTCATAAATTCAGTCCAAAGCTGCAATTCATCATCAGGATTTTTATACCTTTCAAAAATCTGACTGGTGATGAGGCGAGTCGCACAGTCGGTAATTCAATCCATAGGTGCAAAGCAGAGTTTCACTATTCCAAGAAGAAAAATAAAGCTGACTTAGTCTACGGAAAGACTCAGAAAAAAGCAAGAGAACTTTTGTCATAAAAAGTTTTGACAACGTCTTGAAATTTGTTATAAACTAGAAAATTTATTTTTTTGTTTGTGCATGGAAAATTTTTCGCAGCTTATTGTAGGAATTGCTATCTGAGGAATTTTGGCATATTCTTTGGCAAATCTTGTTTTTCATTGTAAACTTTCTGCAATCAAGAAGAAGTCTATTTGTCATTCGAGAAGTTCTATTTTGGGAGAAGTGACAGAAAAAGTTGTCCCGCTTTTGCCAGAATTCCCTTATCATATGAAGGATTTAGTTTTTGTCGGAAAGGGGATTGATTATATCGTATTTGATGGGCTTTCGCGTGGGAAGTTGAGAGAAATTGTTTTTTTGGAAGTCAAAAGCAACTCCTCTCAGCTCAATAGAAATGAGCAAATGATTAAGGAGTGCTTGGGCGCTTTTTCTGTGAGGTATGAGGTAGTGAGGGTAAACTATTAGATTTAAATCAGATTTTACATCTAGTAGTTTTGAACTTGATCCTCAAGATATGTGAGTTCTACACCTGCATTTTTGAAGGCTTCTTCGCTGAGCTTTGCATCGTGGAACTTCCTTTCGCAAACAACTCTTGTGATTCCACAACTTATAATCAGATGAGCACAATGTCTCACGTAGCAAGGAGTCATTTTGGTATAGAGCGTTGCTCCTTCTAGGCTGATGCCCTGACGAGATGCGTTTGCAATTGCGTTAAGTTCAGCACAGCAATTTCTCATACAGTGTTTTGTGGTTTCATGGGTGTCTTTCGTTACCTCCCGGATCTGATGTCAGACTTCATCACATTTTTTGCTTCCACGAGGAGCATCTACAAAGCCTGTGGAGATTACTTGTCCATTTTTTACAATAATGCAGCCGCTTCTTCCGCGATCACAGGTGCCTTTTTCTCCGACTACGCGGCAAATATCCATAAAATATTCGTCTTCTGCGATGTCTTTTGGATTAAGGTGACCATGGTCTTTGATACTTCCGTTTTGATACATTATTGTTTTTTGTGTGATTAAAGATCAGAATTCTGACTTGTTTTTTTGTGGTTACATTACGAGCATACTTATACCTCCCATTCTGTTGGTTCCCATATTGATTGCAGTATATACTGGTCCATTTTGATTGGCTGTGAGCTTTATTTCTACCTTTCCATCTTTTACAAGGCTGATTGCTACTGGGTCTATGCTAATATTGGTGCTATTGGCTACGAGAACGATAGGCTGTTTCAATACTCCATTGAATTTATCTCAATTAGCTTTGGTAATTTCTAAGGTGAAGATTCTGGTTTCTCCTTTTTTGATTTCAGAACTATTATCTCCCTTTAGACTGATGTTCCATTCAGAGAAGAACTGTTTTCCTGTGGCATCAAGTTTACTACTATCTAATGCAATCAGATTTTCTCCTTTTGATGCTGTTTGATTATTAGTATTCGGTATGCTTTCTGGAGAAGGCTGGATTGGTTTTATCTCGGGCTGTTTTGCAGGGAGTCAGGCACTATTTGGTATTTTACTCAGCGAGAGGAAGCTTTGGGTAGCTTTTTCTTTATTGTATTTAATGCTCAGATATTTGAAGGATCCTATACCCTTGAAATGTTCTTTTCGGTAGCCGTCCATAGTGGTAAGCTCGACCTTTTCATTTCCAGCGTGGATCATAAGTCCGTTTCCGGCATAGATTCCAACGTGTGTGATCTCAACGGCTGCAGGATAGGTATTTTTGAAGAAGAGCAAGTCGCCGATTTCAGCTTGGTTTAGATTGTCGTATCGGTACCCTCCGTTAGAAAACTGATGAGCTTGATCTCCAGCACTTCTTTCGAGCTCGATTCCAACGTTAGTGAATACATTTTGCGTGAATTTGCTACAGTCGGTTGGATTGCCAGGAAGTGTTCCGGTTGCTCCCATTTGGTAGGGACTATCAAGATAGAGTTTTGCAATTTTTAGAATTTGTTCACGATCTGCAAAATCAACTTTATTTACAGGATTTACTGGAGCTGCATTGTGTTCTGGTTCAGATTTTTCACTTTTTTCATTATTATTGGCTTTATGAGGAAGTTTTGCGTTGGCAGATTCAAGCAGAGGAATTGGATCAACGGTAGTATTGAACATTTCGGTCCTACAAAGTCCTTGATTGATGATATCCGTTTCATTCATTCCTTTTTTGAAGTCTGCACAATTCATGAATGCGTAAATTGGTCTTCATTTTTCACCTTTATCAATTTCAAAATGCACGTGATACCCTCCTAAACCTCACATAGTGAGTCCACTATTCCCTACGGTTCAGATTTTTTGTCCTTTACTAACATTTTCACCTTCTTTAACGGTAATGCTATCCATATGTCCGTAGGTTGCGAAATATCTGATGCCTTGATAAACGAATTCGATTTTTACGACATTTCAATATCCGGCTTGTTGCCCAGCAAAGAAAACTTTCCCATCTGCAATAGCGTAGAGAGGTGTCCCTTTTGATGTTGCGATATCTACACCGTAATGGCTTCCCATATCTGTTTTCCAGCCGCTGTTATAACTTCCTCAAAAAAGTACGGTATAAATATCAGTATAATCCGTATTATTCTCATAGTTGGCATAGTTTGCTCCTTTAATGATTGGAAGTTCTTTTTTACAATCAGAGGCTAACGTAGACCATTCCTTTGTTCTACACTCAAGGGTAGAGACCTCAGTGAGAGGATAAACAAGCTCACGATTATTTGGAGTAGCATTCCCGTTTCCAAATATTAGGGTAGAACAACTGAGCACTACCGCAAGTGAAAGCTCTCCAATATAGATCCAGTTACGATGCAGAAAATTAGAAGTATTGAAGGCTTTATCAATTCTTTTTCCTTTGTGGTGTTTTTTGAGAATCCTCGTCCCAAATCTTTTGTGAAGTTTAGCTTGAAATGCTTCTAATCTTTTCCATTGGTTATTCATGAGTCTTGGCATTTTGTATAAAATACAAATCTAATTTTATACACAGAAATGAAAAATGTCAAATTTTTAATGATATTTTTTTGAAATTGGCAAGTTTGAGCTTTCGACTATCTGTAAAGTAGACTATTTTAGATTTTTTTAGTTTTTCTGGTAAATTACTTGATGTTTTGTATAAATCTAATTGTGCTGAATTTCTGTGGTCTTGTTGCTTGTCTATGGGCGCTTTTTGTTTATTTGGATTACTTTTTATCTAAAAAGGAGGCGCTTCATCTCCTTCAACCACTTGACTCACTTTTATGTTGGTTAGTTTTAATAGTGAAGGGATGGAGCTTGTTCGTGCTGGTTCTTGTTTGGTATTCAGATAATTATCCTTCTTATTTCACTTTCCCTTCCACTTCAAGTGGACTGTGCTTGCAATTCTGAGCTGAGTTTGTATACTTTCCACGAGTAAAAAATCAGATTTTAGATTTTTTTAATTATTATGCAGGTAGAACAGCAGCCAGAACTTCGCGTATTTCTCGCAGATATTGTAGATAAACAGACTCCGAAAGCACTCCTTGAAGATAGGATGAAAGAGCTTGAAAACCTCGTGAATACCTATGGTGGGGTGGTGGTTTTGCAGAAATATCAGAAAAAAGATTTCCCAGACCTCAAAACTTATGTCGGGAAAGGGAAACTAGAAGAAATTATCGCAGATATGCTTCGCTTAAACGCAAATTTGCTGATCATTGGAAATGCACTTAAGCCCTCACAAATCTATCAAATCAATGAACAACTCCGCCTCGTGAGCGAGGAGCATAATCTCAAAGACAAAATGGTCGCTTGGGACAGGATTGATCTGATTTTGAAAATTTTTGAGAAACATGCAGAGGGAGGAGAAGCGAGATTGCAGATTGAGCTTGCGGCGATTAAGCATATGGGACCGAGGATTTATGGAATGGGAATGGAGCTGAGTAGGCAGGGAGGAAGCTCTGGAAGCGGAGGCGGAGCAACCAGAGGAATCGGAGAAACCAATACTGAAAGGATGAAAAGGCACTTGAAAGAGAAAGTAGGGAAAATAGAAAAAAAGCTGATTGAATACGAACAGATGAGAAAGCTCCATCGTGATGCTCGTAAAAAGAAGGGGATGCCTACGGTTGGGATCGTTTGATATACCAATGCTGGAAAGTCTTCTCTCCTCAATGCTATGACCAAAAAAGGCGTTTTAGCGGAAAATAAGCTCTTTGCAACGCTTGGAACGAATGTCGGGAAATGCTATTTGATTACCAATCCTGAAACCTGACTTGGAAAGGAGATTTTGCTCAATGATACGATCGGTTTCATCCGTGATCTCCCTCCAAAGCTGATTAAGTCCTTTTCTTCTACACTGGAGGACAGTATTGAGTCAGATTTGTTGCTTCATGTGATTGATGCGAGCGATCCTTTTGTAGAGGAGAGGATTGAGGTTGTGCACCAGGTTTTGTCTGATATTGGAGCGAATCAGGATAAGATTTTGGTGTTTAATAAGATTGATTTGATCAATGAAGAAAAAAAAGCTGAATTAGCTCAGAGATTCGCTGATTTTCCAGTGGTTTTTGTTTCGGTCAAAGAGGCGGTTGGCTTTGAGCAGTTGAGAGGAAGGTTAGTAGAGAAACTGAAGTAAGCTTTAGTTTTGAAAGTTGGTTCCATCTTGATTTTTGAATCTTGACTTTTGTCTGTTTTGTTTTCCCCCTTTTTGTATTTCCACTTGAAAAGGAGGCTTGTTTTTGATATACTGTGGAAGTGAGTTTTTTTAGCTGTTGGGAAAAATTAGAATATGTGGCGTAGAAAGTTCGTTCATAGCTTACATAAATTCAAGTCTCGGTTGCGGGTTCAGATGGAGAAACTTGATGCTATTCCTTATGTAATCAGATTGCCTCTCGGAGTGTTTTTTTTGATCATTGGGATCATTTTCTTTTATATCCCGTTTATTAACGGTCTCATCTTTATGCTTGTAGGAGCGAGAATGCTCGGTAAAAGGCATATGAATAGAGTTTTTCGCTGGGTTTCTTCTCAGAAATCTTATTCTTCTTCTCGTGAAGCACTTTTCAATCTTTTGAAAAAATAACAGCTTTTGTTCTATCTTTACTAAATCACTATGTCTGAAAATACTAAACTATTGGCTCTTCTGGATACCGTGATTGACTGTTATCTTGATAAAGGTGAGCCTGTCGGCTCTAAGTTTTTAAATTCTCTTGAGGCTGTAGATTATGCACCTTCTACCCTGAGGAAGTATTTAAATGCTTTGGAGAAGCAAGGGATGGTTTATCAGCCTTATAATTCTTCTGGAAGAATCCCGACATTGCAGTGACTCTCTGCTTATTTGGATTCAAGATTGTCTGAGCGTCCAGATGATTTCGATTTGCCGCTTAATGATGCGAGGAAAGGCCTTAAAACCTTGGTGGAAGCTTTGGGGGAAGTCTCAGATGGGGTAGTAGTTGGTTTTATTAGGAATGATGAATATTATTACTTAGGAATTAATAATTTGCTTCGTGATGATATGATTGATGAGTATTCGGCTACGAGAACGATTGTTCGTTTTATTGAGGAGAAAAAGGTTGTTGCTTTTATTGACAAGAAAATACTGAAAAATCAGGAAGTTTATTATACGTTTGTGAATGATACAGATACTCTGATTTCTTGTGTTTATGTAAAGGTTAATTTGGAAGGGTATGATGCGGTGCTTTCTATTGTAGGGCCTCTGAGGGTGAATTATAAGAAAAATATTTCCATTCTCAAAAAAGTTTTAGAATCCTTAGCAAATTAGGATGAACAAGGAAAAAAGTCAGAATTTTGAAGCTGTATCCGCCTCGTCACTTGGAGAAAGACAGGAAAAACAGAAAGAACTCCAAGTTCGTATCAAAGAAATGCAGGAAAATTACTCTGCTGTGAAGGAAAAAATGCACAAAGAAGCTCGTGATTTGCAGGCTGATATTTTGTTGACTGACCTTGATAAGAGGATTGATCTCTTGGATGTGAAAAATAAGGTTGTTTTTGACTCAGAAGTGGAAAAAATTGAAGCGGAATTGGCAGTCTTTGATGAGGCTCATCGTTCCTTTTCTAACTTAAAAGGGAAAATTACCGCTCAGCATACCCAAGTTTATCAGCAGATGCAGAACCAATTCCCAAGCAGCTCAAATCAGGCAAACGAGGGTAGAGCAAAGAGCTATGAAGCGATTGCAGAGATTAAACCTCAGGACGGGGAGAATAAAGTTGCGAATTTTTTTGCTGGGATTGTGGAGAAACTGGTAAGTTAAAAAATGATTTATTTATATTTAGTAGTGAGTATGATTTCGATTGTGCAGAGTGCAGGAGGTATTGTTTATTATCTTTGAACTGATGGAGAGCCTAAATATCTTTTGATAAAAAGGCATGCTCTTTCTGGAAAGATTGAATGGGTCGCTCCCAAAGGGAAAATTCAAAAGGGAGAAAAAATAGAAGATGCAGCATTAAGAGAAGTTTCTGAAGAGTGCGGTATTCCTTTGAAATATTTGAAGTTGAGGCAAAAGGTTTGAATGACTTCGCTTAGAAGTTCTGAAACGAAAAAAGGGCATTTGGATAAGGATGTTACTTATTTTTTGATGAAATATGAATGAGATCCAAGTCAGATTCAGGTTACAGATGGAGAGGGGTATATCGGCATCTACAAATGGGCGGATATTCAGCAAGTGCTTGGACTTTTGTATTATGAAGATATTAGGGAACTTATTAGGAAAAGTTATTTAATGATTAAAGAAGAAAGGAAAAATCGCTCAATTCAGCAGGACTTTCTCAAAAAATTAGGATAATCTGTGTGTTATTGTTTGCTTGTTTATGCTTATTCAAAATATAAAAAATCTTGGGCGATTTGGGCTTGCATTTCAGAGACAAAACCATAGGCTGAAATCTGATTTTATTTTTTTTTATAAATACGAATGGCATATACTATTCAAAAAGTGCGAGCAAGAGAAGTGCTTGATTCTAGAGGAAATCCTACCGTTGAAGTAGAATTGACCCTCGCAAATGGCGTAATGCATAGAGCTTTAGTTCCTTCTGGAGCTTCTACAGGAGTGCATGAAGCTTTGGAGCTCAGAGATGGAGACAAGAGTAGATACCTTGGGAAAGGGACTTTGAAAGCGGTAGAAAATGTGAATACAACAATTGCAAAAGCAGTTGAAAATCAGACTTTTGATTGTTTTAGAGCTTTAGATAAAGCCTTAATTGAGCTTGATGGCACTCCAAACAAATCCAAGCTTGGAGCGAATGCAATCCTCGGAGTCAGTATGGCATTCGTAGGAGCCTGTGCAAAAGCGGAAGAAAAATGGATCTACGAATATCTCGGAGAAGGAAAAGGAACTACACTTCCTTACCCAATGATGAATATTCTCAATGGAGGTTCTCATGCGGATAATAATGTTGATATTCAGGAGTTTATGATTATTCCAAATGGAATGCCTAATTTCCACGAAGGACTCAGATGTGGAGCTGAAGTTTTCCACAACTTAAAAGCGATTTTGAAGGCAAAAGGGCTTGCGACTTCTGTAGGAGATGAGGGAGGATTCGCTCCAAATCTCGGTTCTAACGAGGAGGCGCTCCAAGTGATTGTTGAAGCAATTGAAAAAGCTGGATATACCGGAAAGGTTTCATTGGCATTGGATGTAGCTTCTAGCGAGTTTTACAAAGATGGAATGTATGATCTTGCAGGAGAGGGAAAAAAGCTGACTGCTGATGAACTTGTTGCTTTGTATCAGGATTGGTGTGAGAAATATCCGATCATCTCTATTGAAGATGGAATGGCTGAAGATGATTTTGAGGGATGGAAAAAGCTGACTGATGCTTTGGGAGAGAAGATTATGCTGGTAGGAGATGATCTTTTCGTAACCAATATCGCTAGACTTCAGATGGGAATTGATAAAGGAATTGCAAACTCAATCTTGATCAAACTTAACCAAATCGGATCAGTATCAGAAACCCTTGATGCTATCACTATGGCTCACGAAAACGGTATGAGATCAATTTCTTCTCACAGATCAGGAGAAACCGAAGATACCTTTATTGCTGATCTTGCTGTTGCGATGAATACCGGATTTATTAAGACCGGATCTGCTTCTAGAACTGATAGAATCGCGAAATATAACCAACTTTTGAGAATTGAAGAAAGACTTGGAGCGAGTGCGAAATATGGAAAATAACTTGACTTGCTGTTGTGAATATGAGAGAAAAATTCTTAGCTATGCAAAGTTAAAGCAATTGAAAAAAACTGACCTATAAGTAGAGTCAGTTTTTCTTTTTTAGTGGAGCTTTTTTTAGTGCTTTTTACCTAATGTTTCTCCAAGACCTTTGATTGCATCTAAGATTTCAATAGGAACCATCCAGATTGTCGTATTGGACTGGTCTGAGCTAATGTCATTTATGGATTGTAAAGTCCTGAGGTGAAGGGCTCATGGTGCTTCGGCGAGCATTGTTGCTGCTTTCTTGAGATTCTCCGCACTTGCGAGTTCTCCTTCGGAGGTAATAATTTTTGCTCTTTTTTCTCTTTCTGCTTCTGCTTGTTTTCCGATGGTTCTTTTGAGGTCGTCAGGGATATTGATATCCTTGAGCTCTACAGAGAGGACATCAACCCCCCAAGCATCAGATTTTTCATCAACGATTTCTTTTATCTTACTAGCGGCTTCTTCTCTCTTTGCAAGCAGTTCATCCAGCTCAAATTGTCCTACGATATTTCTCATTGTCGTAAGGGCAAACTGTGTCATAGCATAATCGAGGTTTCTTACGTTGAGAACGGCTCTTTCTACTTGATCATCTTTGATTCTATAGTAGATCACCGCGTTTACAGTACAAGAGATATTGTCCTTTGTCATTGCCTCTTGTGATGGTACATCGACAGCTTTTTCTCTGATATCAACATTGATCGTCCTTTCTACCATTGGGATGATGAAGTTGATTCCTGCACCGAGTGTTCTGGTAAATTTTCCGAAAGTGAGTACCACTCTTCTTTCATACTGGTCTACTATCCTTACTGTCATTACGACAATGAAGATGATGATTCACAAAATAATTCGTACTTCCATGGGTATATAATAAAAAAAATAAAAACTGATTATTCAGAGAGAGAGGTTTTGACTGCGATAAAATCTCCTTTTTGATGTTTACTATTGGAAAGGAGAGCTTTAGGATTTTCAAAATCTTCCTCCTGGGTAAAACAGTGCATTAGTGACTCTTCTCAGGTTTCTTTTGCTTTTGATGTGTCGATGGAGCTCAGCTGTCCTAGAAATTCAACAATATTGTCGAGACCTTTTTGGAAACTTTCTTTTTCTTGCGGAGTAAGGTTAAGTTTGGAAAGCTTTGCTAGATGCTCAATCTCTTTTTCAGTAAGCATTCTTGGAAACTTTTTAGAAGATAAATTTTTTATAGCTGTAGTGCTTCAAGTATGCAAGAAGAGCATTCCTCACATAGGCTACAATTTCCCTTATATCTTAATACAGAGAAAAGTCAAGCAAAACACGAAGAAGGGAGTCAAATTTTTTCGCTTTTGTATTTTTTTTGAGTATATTTGAGAGGTATATTTAAATTATTTTTTTATTCTTAGAATAGATTATGAAGGTAGTATGGTTTGGATCTTTGATGGTCTTTAGCTTGAGTACATTGCTCTCACCTCAGGTTTTTGCACAATCTGGAACTTCTTTACCTGGATGAAGTGCCGTTTCTGTTGCCTCTCAAGAAGCAATGCTCCAAGATTCTGCATTTAAGACCGCGGTTGATCAAGTGGCAGGATTGAGATTGAGTCTTTTTGACTTCAAAAAAAAGCTTGATCAAATGGATTCCAGTACTAAAGAACTTCACGCTGGAGAGTTTGATGCAGAATATAAGCTCATCAGGGCAGAAATGGTCAAAGTTATTCAAGATATTGATTATTCTACTGCTAAAATTACGAGAACGCTCAGGACGCTCTATACTCATAAACAAAATCTCCAGAAAACTCTCGATGAACTGCAAGAGACGAGAAAACATTTGGAAATCTGAAAGAAGTATCTCAATCAGCTTTTGCTTTTGGTTTATAAAGTGGAGAGAGAAATTTATGACGAAGAGGGCGAACAAATAGATGCTGTTAAACTTTTTATCAAATCAGATGCTATGCCTCAGCTACTTGCTGGTGATGATACGCTCAAGATTTTGTTAAATCAGATTAATACTCTGATGAAGGAGGCAACCATGCAAGAAAAGCAGAAATCTGATACTATTGATAAGTTTGTTAAATTAAGAACTCAGGCTCAAAAATCACTTGATTATTACAAGACAGAGATTAAAAAACTTGAGCAGAAGAAGGCGTATTTGATGAGTTTTATGCAACTGTATAATGAAAAAAAACTTTGAGCAGAAGCAGTGAGTGGAGTAAGTTCAGAGGAAGTGAATCTTCATGAAACTATTAATACTATGGTCGCTGATATCGTAGAAAAGAAATATCTGACGACAAATGATTTGCCAGAGAAAATAAAGCAATTGGCGGATCATACTGATAGTTCTGAAAATGAAACTTCTCCAGCTGCTTGGCCAACTTATCCAATTTCTCAGATTTTGACTATTTTTAAAGATCCGGCTTTTGAGAAGGAATATGGATTTAAAAATCTTTCTCTCAAACTTGCTGTAGAACAGGGAACACCGGTTTATGCGATGAGAGATGGTATTGTTTATTATACAGATACTGGTTCATGAAACGTGAATCGAATAATGATTCTTCATACCGATGGTTATATCTCTACATATGCGTATTTGAGTAGGATTTTTGTTCAGCAAGGTGATTTTGTAAGAAGAGGTCAAATTATTGCACAGAGTGGAGGAGAACCTGGGACTGAAGGTGCTGGATTCGTTTCAAAAGGAGAAAATCTGACTTTTTCACTTTTTAAAGATGGAGTAGCGATTGATCCGCTTACGGTCCTTGATCTGAGTGTTGTTCAGGATAAAGATAAGGTTTTGCCTGAGGAATATCGCTTGAAATACTTTAATGATCAGTATGTTAGACCAATTGATATTACCAAGGTATCGCTCATAAAGGGGGATTCTGTCGATGAAAGGGCTCAGACTTTCCTTAATTCTTATGCAGTAGGTACGTATAGAAATGTTGCATTTTGGGATCAGGTCGTTGCTGGAACCAATATTGATAGAGATATGGTGATTTGTATCGGTTTTGCAGAGTCAACTCTTGGAAAATTTCTTGCTACGGATAATAATATCGGAAATGTCGGTAATAATGATAGAGGAGATCGTATTGCTTATAATAATCCGTTTAATGGAGCGAGGTTGATTCCTTTAACCTTGAATAATCAATATCTTGGAAATTATCATACTATTAAACAGCTTTCGAGATATGGAAATAGTGATGGCAAGATTTATGCCTCTAGTCCAATTAATTGGCAGAGTAATGTCCAGAAATGTCTCTCCAAGATTAAGGGATACTATGTTCCTGAGGATTTCCCTTTTAGAACTTGACCAAATCCTAATACTCAAGCAGGAGCTGCTTTCCTTAGAGAGGCAAAAGAAGAAAAGACAATGCTCATGAGTGGGTCTGTGATGAAAAAAATTGGAGGAGTTGAGGTTTCCTCCCTCTAGTTTTTTCTCAGGAGAAGGACTTATGGTTTCATATTGTAGGGACATTTATTTCTTTATCTTTTCAATATATTGCTTTTCTGTAAAAAATAGGTATAATATAGTATGCTTTATCTCTTGTTTCAGAAAATAAAGTTTGACTCATGCCACAGCAATCCACATCTAGATATCGTTATTTTCAAATCAAACCATTCAAGGACAATGAACTTTGAGTGGGGCATTGGGAAAAACTCATAAAAAATTTCGTATCTCTCAAGGCGAAGAAGATCAGCTTTTTTCTTTATTGAAATAGTGCTGAAATTCAGCTGCTTGTCAGGGTTCCTAAGGATTTTGAGGCTTATTTTCAAAATACTTTTTATACGACTTTCACGACTTCAGACCTTGAAGAATGTGAGCGTATTTGTACTTGAGGAGAAAAAGTGCATCTAGGATTGGTAAAACAGAGCTTTTTTTCTTGAAAATCTAGTTCTGGCGTCTTGAAATCGAAGGAGGAATTTTCAAAGGACGGTTCTTATCTGGATCCTATGACGGATATTTTTGCGGTTTTCCAGAACGTGGATAGGAACTCAATGCTCAAGATTGCTTTTGAGTATACTTTTAAGCTTGAGGACAGTGATTTGACGAAGCTCTTCTCTTTTATAGAGAAAGTGTTTAGCTATCTTTGGTGGGGAAGTCAGAAGAAAGAAGAGTCTGGTGAGACTAAAGAAGCTCCAAAGGATTTATTTATGAGCATTTGGTATAGTATTCAGACTCAGGATGCTTATATGAAGGAGAATCTGAATGCTCTTCTAAATTCTGTATTTGCACCTTTTCTCTCCAGCGGAAGTTTTGTAAAAAAAGAAAAAACTGAATGGATCCCGGCTAGTTTTTCTCAGGTGATCAATTTTTTTCATTTGCCGACCAAGATTAACTTTATCAAAGGGCTTGGTTATACGGTCTATAGAAAGTTGCCTTATCCTACTACGCTTCCTACTCCAGAAAATACGCCTGCAAAAGAGCTTACTCTCCTTGGGGATACTGATTACAGAGGTGATAAGATCAGATTTTGAATCCGTGAAGAAGATAAATTTAGGCATATGTATATCGTAGGTAAGACCTGAACTGGAAAATCTACCTTCATCAACAATATGATTATCTCGGATATGAGGGCAGGAAATGGGCTTTGTCTGCTGGATCCACACGGAGAGCTCGTAGATGATTTGCTGGAATATATCCCATCAAACCGCATCAATGATGTGATTCTTTTTGATGTTGGAGATGCGGAATATCCGATCGGGTTCAACCTTTTGCAAGCCGATAATGAAGACGAAAAAAACAGAATTGCTTCTGGGGTTGTTTCCACTTTTCAGAAACTTTTTGACAATTCCCGAGGTCCAAGGCTAGAATATATTTTGAGAAATGTGGTTTTATCAGTAATTGACTATCCAAATGCAACCTTGATGCATATTCTGAGAGTCTTGACGGATAAAGAATTTCGTGAAGAGGTGATTTCCCATATCAAAGATTCAGTTTTATTAAAATTTTGGAATAACGAATTTAATAAATGGCAAGACAAACAGAGAGAAGAAGCCGTAGGACCGATTACCAACAAAGTCGGACAGTTTCTCTCTTCTCGCCTTGTGAGGAATATCTTTGGGCAGCCAAGAACGAAACTCAGTATCAGAAAAGCAATGGATGAGGGGAAGATCATTCTTGTGAACCTTTCAAAAGGAAAAATCGGTGAAGATAATGCTAATATGATTGGATCGCTCTTGGTAACGAAAATTCAGATTGATGCGATGGGAAGAGCCGATATGGCGAAACATTTGAGAAAGCCGTTTTACCTTTATATTGATGAGTTTCAGAACTTTGCGACCAAGTCCTTTGCGACGATTCTTTCAGAAGCGAGAAAATATAAGCTTTCTCTCATCGTAGCCAACCAATATACCTCTCAGCTTGATGAAGCGATCAAAGATGCGATTTTTGGAAATGTAGGGACGATTTTTTCCTTTACCCTTGGATATGATGATGCTTCTGTGATGACTTCGCAGTTTAAGGAAATGGTCAGCACTAATGACCTGATTTCTCTGCCAAAGTTTACCGCTTATACGAGGCTGATGGTAGATGGAATCTCTTCAGATCCTTTTAGTATGAAGACTTTGCCGCCTTTTGCATTGGAGGGGGATGTGGAGCATATTGAGAAGATTAGGAAGCAGTCTCGTCAGAGGTATGCAATGGAAAGAGAACAGTTAGAAAGTCTGATGAATGCTCGAAGTAAGAAATCTTTTTCAGCGCAAGAGAGGGTGGCAGAGCAGGCGAGGTATCAATGAATTGGACTTTCAGAACAGGAAGCTAAAAACTTACAAGATTTCCAGGTGCAGCAAAATATGAAATGGTTTACCGATCTACAGATTGGATGAAGACCAGCAGATGCAATGATTGTTGATACGGAATGGGGAAATCATAAAATGATTTGGTATCAACAGCCTGAAGGTATCGAGTCGATCGCGAGTTTACAGGTGCAAAAGGGTAAAATTGTAAAACTGGATGCAAATACTCAGCTTTCTTTCTTTGTGAGTATTTGGCAGCATAAAAGCCTGCTTTGCGCTAATAAAAAACCGCTTACGATTTGGGTCGGGACGAAGACTGATGTAGAACGCCAATTGAAGGAAATCTATCAGAAATCTGGTTTAGATGAAGAACATTCAGACTTTTTTAAGTTTGTGCCTAACGTTGATCTGATGACGGCTCAGCAGGAGCAGAAGAAAACCTCTCAAAAACCTTCCTTATCAAAAGCAGCGAAGCCTGCTCCGCGTAAGGCTCAGGCTTCTGTTGCTCTTTCACAAGCAGGGTTTTCTGTCCATGATGTTCAACTTGGGCAAGAATATGAAGGATATGTGAAGCTGATTTATAACTATGGGATGTTTATTACCGTTAAGGGGGTAGAAGGACTTCTTCATAAGAAGCAGATCGTCACGCCAGATGGAGTAGATTGGAAAAAATACTATAATATCGGGGATAAAATTAAGGTCAAAGCTTTTCAGTTTAAGGAGATTGACTGAGAAAAAAGAGTCGTTTGGACTCAAATGTAGTCACTTACGGTAGTTCCTTAGGTAAAACATCCTGTTGAAATGATGAAAAATCTGACTCTGTTGATTAATGGTGTCAGTTTTTTGATAAAAGAAGATAAATTCTCTTGCCTTCCTTCTGCTGATAACTACACTTGTTCTGGATTTATTTTTTTGTTATTGAGATAATGTTGACGAAAAGGCAGATTTATCTTGTGATTTTTGCGGTGCTTTCTGTGCTTTTGGCGGTAGGGACGCTTTTTTATCTTAGGGAGCTTGATCGTAGGCTTCAGGAACCTCTCCAAGAGGAGTTTATCTATGAAGAAACGCCTGTAGTACCAGTTTCAGATTTATCATCTTCTTCATGAGCGGACTATTTACAGGAGGATTTTGATGCTTTGCTCTCTGGAGAGACGGTTGCAGATATGTATGAAGGTGAGCGTGATTATGGTTTTACCGGGAGTCAAAACTAAAGCATGGTAGCATATCATACTAATGCTGTATCGGAGCTTCTCCTCCAAGAAGATTCAGTTTTTGAACTTTGGGATGGAGCATGTGCTGAGAAGACGATCTTACTAGGAAATTCTAAACCACTCAAGTATGCTGCACTTGTGCATGCCAGCACGCTTGCTTTTCAGGTCAATACGATCGAGGCAGGAAGCGAACTTGAGATGATGCTTCTGTGTCCTGTGCTGGATACTCGTGAAACCTCGGTTAATCTTATGCTTAATCTCAGGCATAATGATTGTAAGATTCATCTCCATCTCGTAGCGCTTGTTTTTTCTGACGCGAAGACGACTTTGAATGCTACGATTTTTATGGAGCCACATATTCAAGGCGCGGCAAGTAAACTTTTGGAAGAAACGGTTGTGCTTTCTCCTGAGGTCCAGCTTCAGAGTCTTCCGATTCTTGATATTCAGGCAAAAAACATTCAAGCGGCTCATGGAGCGAAGATTTATCGTCTCGACCAAGATACGCTTTTCTATCTGCAGTCAAAGGGATTATCCTATCAGCAGGCACAATACCTCCTTCTTGCTTCCTATGGGGAAAGGCTTTTTGAAGGAGTCGCGTTGACGGAGGAGGAAAAATGAAGGTTGGTTTCAGATTTTTTAACTTTTGATTTGTAGCGAAGATGAAAGCATATTTTGATTTGGTATTGGATTTGCTTGAGATCGAGGAGAAGGAGCCCCTTTCTGCACTTGCAGAGGAGCTTGTGCTTGCTCATCAGCAAGGCAAACGCATCAAGATTGCTCATCGTCATCAGGTATTGTTTGAAGGGCGGCTTTTGCTCTTGGCGGGGAAACTTAGTCCTGAGGGGTTTGTGCAGATTGGTGATGTTGAGTCAGCTTTGCCTCTTTGGAAAGAAGAGGGGAGTCGTGAACTTCTCCAACAGCTACAATCCTGAATGCTTCCAGAGGAAGAACTGATTATTATTGATGAACGTGCATGGAAGCTTTTCCTTAGCCCGGACCAACAGCAAGAGCTTCTTGATCTCTTAGAAAAAGAAAATAAAGCAGTAATTGTAAAATAGGACTTGACCCGTTGTCCAAAATCTGTATATCAGGTAGTGTTTTTATCTCATTTGTTTGTTTGCTCATGCCGAATAAAAATCTTCAAGCTTTCACGAAACTTTCTCAGAAGTATGTTCGTAGGTTTACTAATTATACCAAAAATCAAAAAGCTTTTTCACTCTTTTTGATGGTGTTTGCTTTTGCGCTGGTGGCTTTCCCGCTGGTGAAGATTGCTCCAGTCTTGATTTCGAAGAATGGGCAACCTACGATGGCAGATGCAAGTGATGCACAATCAATTTATCTCCTTGGGAAGACCTACTTTAAATCTATGTTGATCATTTTTTCGAGTCTGATTTTTTTGCTATGATGGAATATGAATATCAGGTTTAAGGGCTTTATCGTAAATTTCTTGGGATTTAGAGAGAGTGAACCGATGCTTAACTTTGCTTTCCTCTGGGTGATTGCAAGTGTCTTTATGGGAATCCTTGATACGCTTGGTATGCTTCCTGAGGTTACTGGTCAAATTACGCTGGCTCGAGGAGGAAAGGTGATGTTGATTCTGCTTTTGATTGGGTTGGTGCTCAGCTTTATAGAGGTGTGGAAAGGTGCGAGTAAGAACTCTCAAAAGACTAAAATTCTGAATATCGTCGATGATGAAGCTCCTTCCAAGAGAGGTGATGGCAAAAGGGTGCTTCAACATCTCTTTGATGATGAAGACTTGGAAGGTTAGCTTTCGTTATGAAAGGCTCTACTCCTTTGCTAGGGGGTAGGGTTTTTTGTTTCTGGGGATTGAGGAGCGGGAGTGGTCGCGGTAGAGGAGGCGTTTCCGGTATTTTTTGAGGCTTCTTGTCCGAGAAAATTTTCCATTGCATGGAGCTTGAGGGGGTTCCAATGCGGCACGAAAAAGCTGACGGTCTTGCCGACATAGCTACAAAGTGAGATGGGATCACCAATGATTTTGTAGGTGTGGATCTTTTGGGTTCGGTCTTTTTTGAGGAGGGTGTCTTTGAGCATTAAGATGAAGATTTTGTTGGGGGCGGAGCCGGGGTTGAAGGTGCAACAATAATCAACTCTTCTATGTTTGGCTACAATATAGCAGAGGGTCCCTCCGAGTGAATGTCCGCAAATTCGTTTTTGGTAGTCGGGATGGCTTTTTCTTACTTGGTCAAAGAGCTTGAGGCTTCCGCTTACTCTGGGGTCAATCGCATTTACTCCGAGGATAATCTGAGCGTCGGAAAGGAGATCAGATTTTTCTTTGGTATCTGTCCCCCTATATCCAATGATACAGATTTTTTGGGTATGGTTGAGATAGATGCAATGAAAGAGATCGTTGTAGTAGGGCTCGAGATAAAAGTCGCCGATTTGATAGGGTCTCTGTTCTGGGTCGTAATATGCGTATCTTGTTACCTGTGCACAAAGCTCGTCCTCGAGAGGAAGGACAATGTTTTTGCTGATCTTACTATTTTTGATAAGGGTGGTGAGGGTATCTTTGGTCGTAGGAGAGAGTTCAGTCAGGTTTTTTTCTACTGATTTGAGCTCTTTATTTTTGGGAAAAGCATCAGAAAAGAAATTTTTGCTTTTCTGTATTCGTTGGTCTGGGAGATCAATGAATCGTTTCTTAATCTGCATGTTCCTTGAAGAGGTCTAAAAGGTTTTGGTTGCTGAGGAGATCAATCATCGCTGTCATAACTTCTTCTTTTTTCTTTTCATCCATGGTGGGTTTCCCGCTAAAAAGCTGTCCGAAGATACTGGTATCACTTTCATTGATAAGGTTTTGCTTGAGGTAGATGGTCGTCCTATCGATTGCAATGTTTGTGACGCCGAGTTCTTGCACTTTGTTGATTTGCTCTTTGAGGGCGGGGGTAAAGATCTTGAGGTCGTAGGGATTGAGATAAGTCACCATATGGTCTTGCATGGATTTGCTGTCCCTCTTGGTGAGATCGAAAAAGTATCTGATGAATTTGTAGGCAAAGAAGAGAATGAAGATGAGCAACGTAATTGCATTGACGATCTGAATGATTTTTCAGATTTTTCCTGGTATCATTAATGAGAGTATCATAAAAATCAAGAGGGATACCATAGGAAAAGATTTTATAAAGCCGAAAACTTCTTTGAGGGTGATGGATTTTTTTTGGTTGAGCTTGATGGAAATGAGGGAACGGGTGATTCTGGTCTCTCTTGGGATGGTAATTTTGTAGATAAAGAAAGTATCACTGACTGCGTGGTCGTTGTCGTAGATGTCTGCCTCGGCTCCTTGGATGATTGTGGATCGGTTGGTCTTTTGGAAGGAGTATTTAATATCCATATCAATACTCAAGACACTGATGAACCCTAGCTTCTGCAAAAATTCAATGGTTTCTGGGAAGAAGTCATTGCTGGTGCTGTATTGGAGTCCGAGTTTCGGGGCATTCGTATCGATCGCCATCCGTTTGCAAGCAGGGATAAAAGGTAGTTCTATTATAGTCATAAAGGGAAAAAAATCAAAAATCAGACTGTTTTTGTGGGGATGGGGGACTCTGCACAAAATGAGTAAAGCTCCTCTATAACGCTCTTGATGATGAAGCAAGAGAAGATCTCAAAAATTGAATTATTCTCTGACTCCTCCACAAAGCTGGAGATGCTACGAAAAACACAAGATTCAATGCAAAAATGACTATTAAAACAGCCGTAGATGCTTTTTCCCATGCTATCAACGAAGGATACAAAAGTGGGAAATTTCAGATGTGGATAGAAAAAGGGTTAGCGAATGAGAATGGAAAATTCCAAGATTGAGCTCTAGGAAGGATTATCAAAGAAGGAACTTCGGAATTTAAATCTGCCTTTGCTAGAAATCTTGGAAGATTTAATGAAGTGCTCGATGCAAGGAGAAAGCAGGGGTCAGCTCCCTAAAAATCTTTCAGAGTTTTCTGATGCGATTAGTCAAACTAAGAAGAAGCTTTATAGCGAATATGATGCTTTAGCAAAAAGTGCTGGAGAGGGCGGAGCATTTGTAAGCACGGAATGAATAATCAATGAGCTAAAACACCTAGAAAATAATAGAACCCTTAGGCTTGCTAACCCGGGGATTGATGGAATTACTTCTAATATGAAAAAAGGATTAGAAGAAATAGGGACATTAACCGTTGAAGAAGCCCAAACTTTTTCTCAACATCTTAATCAAAACTTAAAAGCTTTCTATAAAAATTGAAATGCCAATGATATAGGAAGAAGCACTGTAGAAGCTCTGGTAAATAATAATCTGAAAAAAGCAATCGATGAGAGTATAGAAAATGCACTATGACAAGGAGAAAGATATTCTTCCCTCAAAAAGCAGTATGGACAGCTAAGAAATATAGAGGAAGAAGTCGCAAAGAGGGCTCTTGTAGATCAAAGAAGAAACGCAAAAAATCTTTTTGATTTAACTGATATTTATAGTGCAAATCAAGTAATAGATTCTCTAGCAAATCTTAATCCAATAGGACTCATAAAATGAGGGGCAACAAGTCTGATCAAGAACTGGTATAAAAAACTGAATGATCCTAATGTTCAGATTAAAAAACTCTTTGAAAATATCGATAAAGAGATAAGTCAAAATGGAAAACTTAGAAAGAAGTGAAATCGATGGACTGATACGGAAGAATGGATCAATGAAAAATTGGGAAGAAAGGAGGAAATCGATAATTAAAAAAAATCTACCGCAGGGAAAGAGCCTACATCGGCAGATGGAACAGCAATAGACAAGGACTTAGATAAGCTCCGTGAATGAGCTGAAACTACACAAGAACTTACACTCTATAGAGGACTCAAAGAAGGAGGAAAGAAGAAGTTTAGCTTTGATGCTTATACTGATAGTGAAAAAGTGGCAAGGTGATATGGAGAAAAGATTATCACAGAAAAAATTAGTCCAAAAAAGCTGGCTGACTTTAGCAATCCAAGTGATGAACTTTTACAAATCATTAAAGATAACTGGAAAGGTGGGGAATGGTATGATATTGAAAGATTGAGAAAACTTGCAACAGCTGAGAAATTCTCCGCTGGGACAAATATGTATGAAATCTTTGATGATGTAAACGTAAGAAATGCCCTAAAAAATGCGGGGTATGATTATGTAAAATTCAATGATTTTGGAAAGGACTTTAAGCCACATACTACCTATTTTGCTACTGATGTAGAAAGTCGTTGACCTAAATCTAAAGTTCTCAATGAAAGTAGAGAAGAAAAAATAACACTAAAATCCGCTTATCAATGGGACTGAAACCTTGCCCCGATTATAAATAGATTAGGAACGAAAGGAAGTAAGACCATGAAAATATTAGACGTTAGTAATAATGTAAAAACAATACTCAAAAAAATGTGAGTAGAATCTGAAATAAAAGGACATTATCTGTCTAGTAATTGAGTCCAGCACATTCTAAAAAGACATGGAGAATGAGCAAAAGATTTAAAGTTTTACGAGATCCCTGTAAGTATAGAAGACATTAAGCTTATTCCTAAAATTATTTCTGAAGCAGATGAAATTATGCCTAGTCCAAAAACGACTGGGAAATTAAATCGCCCAGCGATCATTTATAAGAAAAAAATGGGAAACAAGTACTACTATGTAGAAAGCGTCAATACAAAAGATGGGAAGTTAGAACCTCAATCAATGTATATCAACAAACCGTAATAAGAAAAAGGCACTATAAGAAGTGCCTTTTCTTGTGAAGATAGTATCTAATGCTTTAACCCTAGATATTACGTCCGAAACGTCATCTATCCATCAAACGATGGGAATTATACTCAAAAAATCCAAAAAATCAAGAGAAAGTTCTCGAAATAAAAGGAACTCTGGCTGAGGTCAGGTTTCTTTTTCTTTTGAAAAAAGATAGCTATTCACCTCTTCTAGCTATCTTTCCTTATACTCCTATGAGTGATTATTTACTTTTATTTGTCTCTCTTACAGTAGTCTTAGGATGTGATTTAGCATAGCTTTCTTTCACATACTTTCCTGTAACAGCAGACCTATAATTTACTGTTGTCTTTGCCATTAATTCAGATCAAAAAAGTAAAAGTGTAATCAGACTCCGAATACAGCAAAATCTTCACTTTTACTTGCAATCAGAGAGTATTTGAGTATACTTTCTGAGGTAGAAGATCACTAAGTAAAAGTATAGAAAAAGTAGTAATCGGAGGTGGTATTCTGATTACTTTTTCTTTATAGTGAAATCTCAAAGAAAAGCAATAGTTAAAAAGGTTTGGTTTTATGTCAAAACAAGCTTGACTTTGTCAAAAATATCTATGACGGAGCCTGATAAAACGAGTCAAGATAATTAGAAAAAGAAGACAAAAAATCTGATTTTCTTTCTTTTGAAAAAAGCCCCACGATTGTGGGGTGCAAAGCAATGGTTAATGGGTTAGTGGGATGGTTTGCAAAAATTAATCATAAAATTTATTTCTTATACGACCCATCATCCAATATCGTATTCATACCGCAATACATATCTTTATAATAAAAAGTATTCTTCGATCAATACAGTCCTGGAATTTTTCAGTTAATCATGCTAGTTCAGCAATAATGTAGAACATTAAACTAGAAGTAATTCCAATCATGAGGTTCTTCTGTAATGAGGTAGGTTTCTTTTTGTTTTTTGTCATAATGAAGACTGTAAAAAACTAAAATTATGATACAGAAATAGATTCCTTTAATCTATAAACCTCAGATTGAAACCTCCAAAAACTTATCTCAAATTCTGATTTGAATTCTTGATCAAAAGAAAAGTTACTTTCTCATAATTTTCAAAATACATTCAAGAATTTAATATAATGATTCTTGAATGTCTCTATTTTTTTCGCATCTCATCAGAGACGATTATTAGCTTGTTCTAGTTGAGCTAAAATAAATCCCATTCTTTTTGAGGTTAATAAAATCGTTGTTTCAATGTTTTCTTTTTCATTAATAGTTATTTCTGTAAATACTAATTTATTTAAATCATGAAAACTATCTTCAAACTGATTTAAGATTGAAGTTAAATGTTTTTGTGTAAAATCTTCTTTTTTTTGATTGCGGTGAATTGCTTTGGGGATAATATATGCTGCCAATAAAATTGATAAAATATTTAATAAATCGGATCGTTCTACAAGATTATGTTCTTCATAAATAAGATAATTAGTCATCCAAAAATCAGTTGAAATGGTCTTTACACTTTTACTAGAGAAGAAAAAAAATAATACAATCCAAAAAATAACTAACAAAAAAGAAGCAATAGTAAACATATTTTTTTCAATTTTTTTAATCATTCTCATTTTTATCGTAAGTAGATACAGCATATTCTATACGATTAATTAAAGTAGGATCCTCATTACTGATAAATTTTACCCTCTTGAACATATCAATTCAACTCTTTTTAAGTTCATATCAGAGCTTTCCAAAAGACTCTGACAAAAAAGATGGTCAATATCAATATCATCAATCTAAAATAACGGTTAATGTATCTCATTGTTTTAATGCATTAAGTTTCCCAGCAAGGAACCTTTCATAAAACTCTTCTCCAGAATTTTGACCATCTTTTATATATCTTGGTCAAGGCTTTCTTGAAAACTCCTTTGCAATATTTATCTTGTCTAGTGTAGTATTAGTTGGCATGATCTATTTCCCAAAAATAAAAGGTTCAATGAAAATTATTTTTTAATTTTTTATATTTTTCTTCTTTCATGTCTACTTTTACCGTATTAGAGATAAGTATAGCTTTTTTTACTTTTTTGTCAAAAGAAAACTGATAAATAAAAGGCAATCATTGTCCCCTTTTGGATTCTCTTGTACTGGAAAATACTCCATTCAAGTATTTAGAATCTTGATTTAACAATGCTTCTATTGTTTTTACATGATCAGCCTTTACTTGAATTCCCCATTTTTTTTCCGTATGTCCAAAATGATTTAAAATCCCCTCTCATAAATCAAAAAAACAAACATATGAAGACTTTGTATCTTTATAATAGGTTAGCCACCACTTACATGTAGTATGTCCCTTTGTATTAGCCATACATTCAGTTAATCTAGGGAAAAGTAAGGGAATATTTGACTTACTTTCAAATAACATATCAACCACCTTTCTCGCGTATTCTGGTATAACTTGCCTTTCTGTATAGTTTCATATCATTCCTCATTGGATATGCTCTGTCTTTATTGTAGAAAAAACATTTTCTAAAAATCCTGATTTTTTAAATAGAGAGTTTGCCTTTTCATTATTAGGTAAACTTCAAGAAATTAAGCATCATGTTCTTTTACTATCATTATGTTTAACCATGCCGTGTAATAAGCTCAAAGCATCGAATCATAATTGATCAATATCAGTTAAGTCAAACTTAACGGGTTTCCCTTGACTAAGAGCTTTTTCATATCTATAGAAGTATTCTATCAATTCATCAGTACTTTCAATAAAGGAGAAATAAGAAGGGGGAGTACATTTAAATTTTTTCTTTTCTAAAAGATCTTTTTTAGAGAAACGATTTGAATAGGAGATTCCCTTGATATATTTTTGACGTTTTTTATATCTCATTAACAGTTTCTTCCTAATCTTTTTATGTCTTCTAGTCCTATATCTAAATTGTTTCATATCATATAATCTTTTTTTCGGAGAAACTTTTTTCATCTAGAATACTATGAATAAAAGAAATTCTCCATACACTATATAAATAGTATTTAAGAAATCAAAAGGAAATAATTTGAATTACTATTTTATATTATCAACTAAAAAACCCAGCCAGCTTCTCCTCTGGAATTAGTGCTGACTCTAGGAGTCGGTATTGGTAATCTCTTGCATAATGAAATAAATAATCATTTTCATTAAAGTCAAAGTATCTCTCTATCTTACCATTATGTTCTTTATACAATTTATTCTCACAAACAAATGGCCAAAGCCTACTCTCTAGGCTTACAAGCCATCTCAAATCAGCATAAAATCAAAATCAATCCCTACAGCTGCAATAAAATACGTTCTTTCATTCTGATTTTGTAAATTCTGGATCAAGCGCAATTGAGATAACCTCTCTGTCTTTAGGTTTCCAGACCATCTTAATAAGAGAGTTTAGTAAGGTTTCTAGTTCTTGCATTTTATAAATGAGCTAAATAAAACTGATTTTAAAAAATAGCAAGCATACTCGGAAATAGTGCTGAATTTTTATCATCCCCGAATTTTAATCTACCTTTCGGTAGATTTAGCAATAGAAATAATAATCTGTACTATATTGCGGTGCTATGTTTGTAAGGGCTTTTGTGTTCTTACAACTGCATAACACGGCGGTATAATTCAGATATCGTGTTGTGCCCCCCTTTTTTGTTATCTTTTGCCTTAGACCAACCTATCTTGTGCAGTATTTAGGTGTATGTGTATAAATACTATATCACTATTTTTTATAAGTCAAGATTCTAAAAGCAATTTTGCTTGCATTCAGAACTAGGATTTCTAATATGATAATGAAAATGAGTCAGATTTTTTTATTTTTTCAAACTTTCTTCATGGACGAAAAAGAACTTCTTCAGCCAAGTAATGAAAAGCTGATTACTCATCCGATAGAAAAAGAAATTACGCAGTCTTACATTGATTATGCGATGTCAGTGATTGTTTCTCGTGCCTTACCTGATACGAGAGATGGATTTAAGCCGGTGCTTAGGAGAATCCTCTTTGGAATGTATCAAATGAACAACTTCTACAACCAGAAGCACAAAAAATCAGCGAGAATTGTCGGAGATGTGATGTGAAAATATCATCCTCACGGTGATTCTTCTATTTATGAAGCGATGGTTCGTATGGCGCAACCATGGTCGCTCAGATATCCACTTGTTGATGGGCAGGGAAACTTCGGTTCTATTGATGGTGATGGAGCAGCTGCGATGAGGTATACTGAAGCGAGATTGACCAAGATTGCAGAGGAAATGCTTGCAGATTTGGAGCAGGATACGGTGGACTGGAGAGATAACTTTGATGGATCGCTCAAAGAGCCTGTGATGCTTGCGACCAAATTCCCAAATCATCTCTGCAATGGAACGATGGGAATTGCTGTAGGTATGGCGACGAATATGGCTCCGCATAACCTCAATGAAGTGTTGGATGCGTCTTTGCTTCTCCTTCAAAAGGAGGGGAAAGAGGAGATCGTGACTGATGAAAACGGTGCTGAGATCAAAAGACCGATTTCGGTTTCTGTTGAAGAGATTATGGAAATCATCAAAGGACCGGACTTTCCAACCGGAGGAATGATCTTTGATAGCAATAATATCACTGAAGTCTACAAAAAAGGAAAAGGCTGAATCGTTGTTCGTGGGAAGACTCACGATGAAGTCTATGATGGAGCCCATGTGATTGTCGTAGATGAGATTCCTTATCTCGTAAATAAAGCCAATCTCGTTTCCAAGATCGGAGAACTTGTGGTAGACAAGAAGATTGAGGGGATTAACGATATCCGTGATGAATCCAATAAAAACAAGATCAGAATTGCGATTTATCTCAAAAAAGGGGTAGATCCAGATGCGATTTTGATGCAGCTTTTCAAGTTCACCGAGCTTCAGACTAATTTCAATCTCAATAATGTGTCCCTGATTGATCAAGGAACTCAGCCGAGACTGCTCAATATCAAGGATCTCTTGATGGAGTTTGTGACTTTCCGTAGGGGGGTGGTGTATCGTAGATCGGTTTTTCAGCTCAATAAGGCTAAAGACAGACTACATATTTTGGAAGGACTGAGAAAAGCGATTGATATTATTGATGAAGTGATTGATACGATCAAAAAGTCAGAATCCAAAGCTGATGCGAGAACTAACTTAATGGAAAAATTTTGATTCTCTGAGAAACAGGCGGAATACATTCTTTTGATGAGATTGCAATCCCTCGTTGGACTTGAGATTCAGAAGATTAGTGATGAAATTGAGGAAAAGATTAGACTTATTGAGGAATTGGAGCGCATTATTTCTGATCCAGAGAAACTTGATGAGGTCGTAACTAAGGAATTCCTCTATATGAAGGAAAAATATGGAGACGAGAGAAGAACTGAACTTTCTAACGATCTCAGTGTCTATAACATCAGCGGAAGCCTCAAAGAATTCCAAAAAGCAGCGGATAGAATCAAGGAAGATGTGATTTGCTGGATTGGAAATGATTTTTCACTCAGAGTGCTGTATCAGACCAGAATTCAGAATATCCCTGAGGAAACCCTTGATCTCATCTATACTCACAACCAGGACAAACTCATCGTGATTACGGATATTGGAGAGCTTGTTGTGCAGAGGCTCAAAGATTTTGGTGCGGGAAATATGGCGAAACCTGCGATCAATCTCAAAGAACAATTCTGACTCAAAGGAAAGATCGTTTTTGCCAAGACTTTGCATTTTCAATATGATTACCTTGTATTCCTTACCAACCAAAATAGTATCAAAAAGACCAAAAAAGAACTCGTTTTGAGCTTCAAAAAATTCCCTACCACGATTATGGGACTTCAGTCAGGAGAAAAAATCTGTAGAGTTTTGCCAGTCAATGACTGAGATAATGTCGGAGTACTTACTCAGCAGGGACGAATGCTCCTTTTCAAATCCGATGATATCAGACCGATGGGAAAGACTGCTGGAGGAGTGAAAGCGATTGAGCTTCAGGAAGGAGATCAGGTTGCCAATATGTTTTTGCATAATGATGAGCCATTTATCTTGATCTACTCTGATAAAAACGGAAAACTTCTCTCACTAGAAGACCTCAAAATCCGAAAGAGAGCGAGAAAGGGACAGGTCGTGATGACAGGCAATGAGAAACTTGAAGGTGGAATCTCAATCATTGAAGGTGCGGTCAGAATTCGTTTTGCTGATGGTAGCCTCAAAACTTTGCATTCCAACGATATTTCGCTTGATGAGCCAGAAACTCCGCTTTACAAGATGGTAGATCAGAAGATTGATGTCGTGTATCGTCCTTGGGAAGAGAAGTCTGAAAATATGAAGTATAAAGAAGAAAAGAAAAAGGCTGAAAAAGCTGAATGAGGACTCTTTGATCTGTCTGCTAATGAAGAAAAAGAAGAAACCGCTCAAATTGCAGAAACTATAGAAACTCCTGAAAATACTGAAAATGTTGAAAATCCACAAGACTAAAACAAGCTGAAAGCTACTCTGATACCATAGAAACGCCAAGGCGTAGTTTTCTTTTACTCACAAAAGCCTATGACCAGAATCAACCTTTTGCCTCCTTCTCAACTCGCAGATCAGCACCTCATTGCCGAATGGAGGGAATTACCGAGGATTTTTGGGCTCGTAAAGAAAAAGCTTGATGAACAGAAGCCGATTCTCGTGTGAAAAAAGTATACGATGGGGACAGGGCATGTCCGTTTCTTTTATGATAAATTGCTTTTTCTTCAAAAGAGGCATCAAGCTTTAGTGAAAGAAGCTCAGAAAAGGGGATTTAAGCTCTCTAAGACTGAAAAAATTAGTTTACAGGGTTTCCCCAAAGTCTATTGTAAGGATTTTTGTCCTTCAGAGGCGGATCTGGCGATCTCCCAGCAAAGAATCCAAGAAAAACTCAAGGCAAAGCCAGATTTTTATACTTTTTATGGGAAGTAGGGGATTGATTTTTTTTGACTAAAGACTAAGTTTCTCTTGATTTTAAGGCCAGAAAGTACGTCTTGTATTTATTTATTCTTTTTCGTTTGCTATGATGTCATATCTTCAGAAACCATCTGCTCTTAAACAGGGTGATAAAGTGGCTATTGTGAGCTTATCAAGAGGACTTTTGTGAGAGCAGTTTTGTGCTCATCAGCTTGAGCTTGGTAAGAAAAGAATTGAAGAAATGGGACTTGAGGTTGTTTTTATGCCGAATGCGCTTAATGGAATTGCCGATTTAGAGGAACATCCAGAAAAGAGAGCTAGTGATCTGAAACTTGCTTTCAAAGACACAACAATTTCTGGAATTATTTGTGCTATTGGTGGAATTGATGGATTTAAAATTTTTCCATATCTTATGGAGGATTCTGACTTTAAAGAGCTTGTCAGAACTTCTCCTAAAGTTTTTATGGGATTCTCGGATACGACTAATCATCATCTAATGTTTTATCGCTTAGGAATGCAAAGTTTCTATGGACCAGCTTTTTTGACTGATTTTGCAGAGATGGATCATCAGATGCTTCCCTATACTCAGCATCATTTGCAGATGCTTTTTTGAAAGGAGGCTTTAGAGATTCGTAGTAGTGATGTGTGGTATGAGGAGAGAGAAAAGTTTTCTCCAGATCAGCTCGGCACTCCCAGAAACACTCACCAAGAAATACATGGATTTGAGCTTTTGCAGGGGACTCAGAACTTTTCAGGGAGACTTTTGGGATGATGTATAGAATCACTTTATGAAATGCTGGTTGGAGGCGGATTTCCTGATCAAAAGTTGATCTATGATAAATATCAGATTTTCCCAACTTTAGAAGAACGAAAAGGAAAAATTCTTTTTATAGAAACTTCGGAAGAAAGGTCTACTCCTGCATTTTATGCACAGATGTTGAGGATTTTTAAGGAGAGGGGGATTTTTGATCAAATCAATGGAATTTTTGTAGGTAAGCCTCAAAATGAAATTTATTATCAAGAATATAAGCAGGTTTTATGTGAAATAGTTGATAATCCTGATTTGCCGATTCTTTATAATGTAAATTTTGGTCATGCATATCCTCGTTGCATTTTGCCTTATAATGCTTTGGTTGCTTATGATCATAATGAAAAGGTGCTTCGTTTCCTTGAGTCACCATTTCAAGAATCGCTTTAGTGGGGATTTTTTTGAGCAGGGCTTGCATTTTCTTTGCATTTCAGTAAAAGGAAGTGATTTTATATTCTATTCTAAATATACTCATGACACAAGAACTCTTGCAAGTAAGCGACATCAAGTTTAAATTTGTGAATGTTTCTGAGGTAATGGAGGCAGAATTGAAGAAGGTGGTAGATCACCAAATTGCAAATAAGGGACAAACTATTCTTAGGAAGATTTATTCAAAGAATATTGATGCTAAGGTGATTATTGACTATACTATTATAAAGAATAAAAGCTGACTCTATGAGGCTGATTTTATTTTTACCTATAATGGAGAGAGATTTGTGGTTGAAAATCATAAGGGCTTTAAAGTTGCTTCAGATCTTGTTACTCATGCTTTTGATAAATGGAAGAGAAAAGTGCTCGGTTTTTGGGGATTTTTGAGTAAATAATTCACTTTTTGTGGGTGAATCTGGGGAAATATTCAGCTTTTATTTTTTTGTTATGACGCTGCATTATGGCAAGTAAAAATTTTCTTACGAGAGAAGGATATGATAAGCTTATTCTTGAACTTCAAGAGCTAAAACAGGAGAAATTACCTGCGGTTTTGGAAAGACTTGCTGAGGCTAAGGCTATGGGAGATCTTTCTGAAAACTTTGAGTATAAATCTGCAATGGAGGATAAGGATTTTATCAACTCTAGGATTGTAGAAATACAGAATCTTATCAATGATGTTGAGATTATCGATAAGGATAAAGATTCATCTAAGTCTGATAAAGTTGTGGATTTTGGTTCGACTGTTACTTTTAAGATTGAAGATGATGATAAAGAATATCAGGTTAGAATCGTTGGAACAGGAGAAGTTGGGATAGAAGATGGAGTGATGATTTCTTTTGATTCTCCTATTGGACAGGCTATTAGAGGTAAAAAGGCTGGAGAAACTGTTAAAATGAGACATGAAAGCGGAAGAAAGAGTATCAAGATTTTGGCTATTTCTTAAGTGTTTGTTCTTTTTTGTTCCTTTGCTGGTCTGTATAAGTTTGTTTTTCATCTCTTAGTTGTTCTTTCATGTATAATCCTTACGATTTCTATTTTAAAAAAGCGAAGCAGGAAGGATACAAGGCGAGAAGCGCTTTTAAGCTTGAAGAAATTCAAGAAAAGTTTTTTTTGATCAAAAAGACGACAAAGTATGTGCTTGATATTGGATGTGCTCCTTGAAGTTGGCTTCAGTATACGATTGGACAGCTCAAGAAATTTAGAGTGGAAAATTATCAGGTGATTGGTTTAGATTTAAAGCCCGTTGAATTACATTTCCCTGGTTTGTTTACTTATGTGCAAGATATCTCACAAAAAGATCAGGTAAGAGCTCTTTTGCAGAGGCATCAGATTCCAAAATTTGATCTGATTCAATCTGATATGGCTCCGAATACTATTGGTTTAAAGGATATTGATGCGATGAGAGCAATAGATCTCTTGGAACAAACGTATTGGATCTATGATGAATTTTTGAGTGATACGGGTGTATTTGTGATAAAAGTCTTTATGGGACCTGGTTTTGAAGAATTTGTATTAAAGCTGAAGAAGAGGTTTTGAGGGAAGAATATAAAGGTTTTTAAGCCTATTAGTTGTAGAAGTCAGAGTAAAGAGACCTATATTATCAAGCAGTATTCTCCTTCCAAATAAAAAAAACAGAATCCTTCAAGGGGTTCTGCTTTCTCGCGGAGTATGGTACTTCTACCTTATTCCACTTACAAGGTTCTACTACACAAAGTTTATTATATATCTTTTTAGAGATTTGTCAAAAAAATAATTATTTTCCTAGGGATAGAGCAATTTTATTTTGTAAATATGTGATACTATAATGGATGCTATAAAGAAACAGCTACGAGACAAACTTCAGGATGATATTAGTGAACTTAATGAGGGGCTTCAGGACTTGTTGGAGAAAGGTGTAAATAAGGGAACAATCGAGGAAGAAGATGTGATTTCTGAGATTGATGACATGGAGGGGAACATTAAGCTGCTTGAAAAATTTTATGATTTAGCGGATAAGCTTTGAATCAAAATTTTGACGATAGAAGAGGTTCTTGAGCAAGAGCAGGAAAGTAGTAAAAAGGAATCAAAACTTGGAAGAGTGAGTCTCTATGGGAAGCCAGATTTGATGGCTGATGGGCAGCATAAGGATTTTATAAAGCTCTATTTTAATGATATTTCTAAAATTCCGCTTCTTACTTATGAAGAAGAAAAGGAAATAGCGAGGAAAATCAAAAAAGGAGATGAAGAGGCCAAAAAAAAACTGATTGAGTCTAATCTCAGACTTGTGATTTCTATTGCTAAGAGATTTTTCGGTTCTCGTCTGACTTTTTCAGATTTGATTCAAGAGTGAAATATTGGTCTTATTAAAGCTATAGAAAAATTTGATCCAGATAAAGAATTTAAGTTTTCTACGTATGCAACATGGTGGATTAAACAGTCTATTACTAAAGCGATTGCTGATATGAGTAAACATGTCAGGATTCCGGTGCATTTGATTGATGAAATGAACTCTTATAATAAGGCTTATCAAATTCTTTTTCAAAAACTTGGTCGTGAACCGACAAGTAAAGAGATCGGTCAAAAATTGGGATTCCCTATTAAAAAAATCAAGAAGCTTGAAGAGGTGATTTTTGGAAATGTTTCTCTTGATAGGGAAGTCGGTGATGAAGGAAGGGATACTTTGGCTGATCTTTTGGAAGATGGAAATACACTGAGACCAGATCAAATTGCCGAAAGGGCTGCGTTGAGAAATAATCTTGATGCAATTTTGTGAATGCTGGATGATAGAGAGGCAAAGATTGTAAAGATGAGGTATGGAATTGATGGACCGAGATTTACTTTGGAGCAAGTTTGAGAAGAATTTGATGTGACAAGAGAAAGAGTAAGGCAGATTGAACAAAAGGTTATTCAGAAACTGAAAGAACATCAAGGACTCCAAAAGATGTTGGGAATTGAGGATGATATAGAAAAAATGAATAGTGAATGAGGAAAGAAGAAAAGGGGACGTAAACCTGCTCCAAAAGTGGAAAAAGATGATGACGATGATTATTATGATGCTTCGTATGATGACGATGATGATTTTGACTTGGATGTGGATTTTAGTGGAAGTGATGAAGATTAATATATTTTTTGTGATTGGGCTGTAAAATCTGATTTTTGAGGTATTTTTTCCTGAAATTTATGAATCAGGTTTGTATCTTTTTTTTGTTCTTGGGGAAAAAATTATAACTCCTACGGTCTTTGGATTGAAAAAATATCGATGAGAACTTGGAATACTATTTTTGGTTGCATTTAACATTCAGAATTTTATACTTCTTGGGTATTGGATTTTCTTTTACATAATTTTTTATGAATATAAATAAATGATGCTGACACCAGAAACTTGATTGATTATAACAGCAGCTGTTTCGCTGACGCTCGGAGGTTTTGCTGGGTTTTATGGGTATAAGAGGACGCTTAATGAAAAGCAGCTTCGCTTTAAGAAAAAAATGGAGAGGGTGAAGGAACTTGAGGAAGAGCTTCTCCAAGAGGCGAAGAAAAAGGCTGAAACTGTCTTAGAACAAGCTGAGGTAAAGGCTCAGAAAATTGAAGATCAGAGGCTAGCAAAGATGGAAGAGATCCAAAATAGGCTCCTTATTAGAGAGGAAAAATTGGATCAAAAATTAGAGAGACTTGAAGAAGAAAAAAGAAAAGTGCTTGATAAACAAAGAGAAGTTGATGAAATTATTGAGGAGCAGAATAAGAAGCTTTCTGAAATTGCACAAATTAAGTGGGAGGATGCTAAGGAGCTGATTTTTAAGAGAGTAGAGGAAGATAATCAGAAAGAGATTGTGAGATTTATTGAAAAATTTAAGACGATTAAATCAGAGGAAGCGCAGAAAGAGGCAGCTCAAATTCTCGCTCAGGCTATGCCAAAGCTTGCTGCAGATACTGTGAGTGAATTTGTTTCTAAAGTGGTGGATCTTCCGAGTGAGGAATTTAAAGGGAAGCTGATTGGGCGTGAGGGGAGAAATATTTCACTTTTTGAAAAGTTGACGTGAGTTGAACTTTTGATTGATGATACGCCATTGTCAGTCAGAATTTCATCTTTTGATAGTGAAAAAAGATTTATTGCTGGGAAAACCCTCGAAACTCTAATTAAAGATGGTAGGATAAATCCTTTTTATATCGAGAAGGTATATAATCAGGTAGTTGCTGATCTTGAGGTAACGTTGATGGATAAGGGAAAAGAAGCTCTTACGGTTTTGAATCTTCCAATGATGAATCCTGAGATTGTGAGGACTATTGGTCAGTTCTTTTTGAGATATAGTTATGGGCAGAATTTGTGGATACATTCTTTGGAGGTTGCTAAAATTTGTGAAAATATAGCGATAGAGCTTGGTTTAGATCCTTTGGTTGCTAAGAAGGCTGGATTGCTTCATGATGTCGGAAAGGTGATTGCTGGAGCTGGAGAATCTCATACTAAGATTGGAGCAGATATGCTTCGTAAGTGGGGGCTTGATCCTATCGTTGTCAACGCTGCAGAATCTCATCATTATGATGTAGAGATGACGAGTCCTCATTCTCGAGTTGTTGCTGCTGCAGATGCTATGTCTGCCTCTAGACCTGGTGCAAGATTTGACACTAAGGACTTTTTTATTGAAAAAATGTGAGAACTTGAAAAATTGATTCTTGAGGTTGATGGTGTAGAAAAGGTTTATATTATGCAGGCAGGTAGGGAGATTATGGTTTTTGTGAATCCAAAAATGATTTCAGATACTCAGGTTGAAGGACTCTTGGAGACTATTGGAAAAAAGATTGAAGAACAGCTTGATTATCCGTGAATCATTAGAATTACTGCGTTGAGAGAGACAAAACTTGTACAATATCTCAGATAAGTATTTATTAAATAGTTTGCTAGAAGAGTGAAGCTTTTCTGCAGACTACTTTATATTATCTTTACTAGTATGTATCCGTATTTTGAACTTTTTGGAACAAAGATTTATTCAACAGGTCTTGGGATTGTGCTTTCTTGTTTAGCTTTTTTGATAACAGCTTATTTCCTTTGTAAAAGATATAATCAAGAGTTCCTTAGGTTGTTTAATGGCTTGCCATGGTTGTTAGTTGCTGTTTATGTCGGGGGACTTTATCTTTCTTTTGTATTGGAGAATGGAAGGATTATTCCGACTTCCCTTGGCTTTTTAAGTCCTTATGGGTATCACTTTAGTTTCGTTGGAGTTATTCTTGCTTGTTTTTGATTCGTTGTATTGTTTATGAAACAGTTTAGAAGGAGTGAAACGAAGAAAGTCTGGCTTGATATTCTGTTTTTTTCTTTTGTGAATGGCATAATTGTCTTGGGAATTGGTTTATTGCTGGGGGATAATTTTGTAGGAAAACCGTATGATTGAGCTTTAGGTGTGAGAGCCCTTATTGCTGAGAGTAGTTTGGTAAAGTATGGAACTGTTTATCCTATTGGGCTTTTTTTGTCGCTCGGGGCGTTGATTCTTAATGTTGTTATTACGTTTTGGAAATTTATAGCAAAGAAGGCGGGACTTTGAATTTGGGGATTTATTTTGCTTTTCGTGCTTTTTATTGGATTACTTCCTTTTTGGAACTATCCTGCACATGGAGTGTGGTCAGTATTTGGCTTATGGACCTTAGATCTCAATTATTATGTGTTTTGATTCCTCATTATCTATTTCTTACTTTGGAATAGGAGGTTGAAAAAGCCTTATTAAAAGATGGATTTGGCTCGATTTTTTGGGTGATTGTTAGTATAAAATGCGACTAAAAGCATGAGTTTTATTCTTGATTGAGAAGCTGTATGGAAAGAAGGGAGCTGGCTGAGCTATGAAAATAATTGAGGAAGGTGGTTAAGGACTTTGGGGTCGACTTTAGAGATTTATTTAGACTGATACGATGAAGTTTTTTTTCCTCAAGACTGATAGTTTGTATAAGATTTTCAAAACTTTGGAGAAAATCCCTGCACAGAAGCCTGTTCAGATTTTTATTGATCCTGAACATCCTTTTTTTGAAAATCAACGATGGGGGAAACAGCTTTCTGACCTTGTTAAAGAAAGAAATCTGAATATTACTTTTCTGGCGGAGAAGGAGTCTAGTAGAAACTATTTTCGTCAGCTCGGTTTAAAGGTGTATTTTGAGGAAGAGAAGCCTATTATTAAGATTTTGAAAACGATTTCATTATTTCTTTTTGATATTAAGAGATTTCATCTTCATGCTTATGATAGGAAAAAATATCTTTTTTATCTTGTGTTTGTAGCTGAAATTTTAGCGGGGCTTGGAATCCTTTGGTTTCTCTTTCTGCTTATTCTTCCGAGTGCGAAAATAACGCTTAAAGTAGCCCAAAATACAGAAGATATTATTTATAACTTTCGTTATTATCCTGCAGGGGAGAGTGGCCATCTTGGTCTAATTAAACAGATCAGTATTCCTTATTATACAGGGAATTTGCAGTATGCATATCAGCTTTCTATTAGTACGGAAAATATCCAGCATATTATTAATCCTTCTGCTGGTTATGTGAAGGTATATAATAAGACATCGAACACTATCAATCTTCTAGCCAATACTAGATTTGTGACGAATAATGGACTCATTTTCCTTTCTAAGATTCCGGTTACGATTCCTGCTGGGCTAGCAGATAATCCTTCAGAGACGAGGATTACGCTTTATGCGAATGATGTTGATGAAGAAGGGCTGATGATGGGGGTTAGAGGGAATATTTCTAAAGGCACAAAATTGACGATAAAAAATATTAGAGATAGCTTTTATCTTGGGCAAATCTGGGCAGAAAGTATAGAAGCTTTCACAGGAGGGAGCACTACTTCTGTTGGAATGGTTTCTGAAAAAGATAAAGAGCTTCTTTCTGCTAAGATTAGAGATGGAGTTTATGCTGATAAATTAAATATTGTAACTAGAGAGTTTAATCTGCCAGGAGCCCTTGTTTTGATGTTTGATTCTTTGATTAAGACGACTTTTCATTCGCTTTCTGTAGATGGAAAGATCGGAGAAAAGGCAACCTCTTTGAGAGGGCATGCTGAGGTTTCTTTTGATTTTTTCTACTTGAAATGGGCAGATATTGTGCAGGCTTTTACTTCTTATGTAAAGGAAAGGCAAGCTGACAGTATTCAACTCATCAGTATTAATCCTAATTCCTTTGCACCTATTAACGATATTAAACACACCACAAGAGATGAGGTTTTCATTATCCCAACCAAAGCGACCATCTTGCAAGGCTATGATTTTGGAAAAGACGTGAAGGGGATTCTCCCTACTATCAAGAATACTGTTTCTTGAATGACTGTAGAGGAAGCCAGGAAGTATATCCTCCAATACTCAGAAATTGCTAGTGTAAAGATAGATTTGGGATTCTTTCATTCAGAAAAAATTCCCGATGTGAGATCGAGAATTAAGATTAATGTGGAATTATAACTTCGCTTTGTTTAGAAGTTTCTGAAAATATCACGGACTGCTTTCATTTTTTCTTTTTCCGCTTCAATTTCCTCTCGTTTGTTTGCTAATTCTGTGTGTTTTTGTTCGAATAGGAAGTTGAATTTGTTGATTTTTTCTAGGACTTGGGTTGCTTTGAGCGGATCTTGAAGATCTTTGTTTTCTTCGTAAAGAAGTGCTCCATTGATATTAACGGTGAGATTTTTGTCGGCTTCTTCCGGTTTAAAGAGGAGCTCGTGTTCTATTACTTCGTTATGGATGAGGTCTTCTATCTTTTTGTGGAGGAGGAGACTGTTGACTTTTCCGTTTTCCTCAAGCTGTGGCGAAATGGTATAGCGAATTACACTTTTTTCTGTATTGTTCCCAAGAATTTCAAAGTCAGAATTGGTTGCTTTTTCAATTTTGTTTTGTAAGGTCAGGATTTCCTTGCTCAGAGTAAAGAGATTTGTATATGCTTGCATAAGATTTGAAAGCTCGGACTTTGGTTCGGATTCCATTTGATGATGTGCCTCTTGTTCGGAAGTTCTTGCTTGTTCTTCTTTATGCTCAGCGCTTTTTTCTTCTTTTTCTGAAGGGGCAGTGTTTGTTTTTTCTTGATTTAACGGCTCTACGTATAATGGAGCCTCAAAAGATACCTCCTCTTCGGTTTCTTCGGATGCAGTCTTTTCAACTGGAGGGAGAATCTCAACATTGAGAGGAGATTCTGAGATAAGATCAGATTTTTCTTCTGGTGCAATTTCTGCTGGCTGGGAATTTCTTGTGAAAGAAGGGCTTTCTATTTGGAATGTTTCAGCTGATGAAGGTGTGGAGGTTTCTTCATTAGGGACTGATTCTTCCTCCTCAAATGAAAAATCAAACTCTTGATCTGCTCCAGTAGGATTGGAAATTTGCTCAAATTTTTCTAGGTTTTCAAAGACACTTTCGTTGCCTCAGAAAAACGATCTTTTGGTTCAGGATTCTTGATTTTGAGAAACTGTATTTGCTGTGGTAGCTGGGGTTTGAGTTGCATCTGCCATTTCTTTGCCTAAGAAGAATCTAAAACGATTTTTATTATATTCAGAAAGCTTTATAAAGCAAATTATTGTTCTTTTTTTGTCGTTTTTTGATAAAAAGATTTGTATTATGAAGACTAAATCATATACTCTGGTTATCGCTCTTAGGCGATTCTTTAATTTATTATTCTTTCGGTTAAATGACAGAAGAAATGGTAATCAGCCAGACAAAATTATTCGTGGGTTGATTGAACTGGAAAATGAGGGGAAATGACCTCAAAACGGAATTCTCTAAATTTGGAGAAGTTGTATCAGCAAGAGTGAATCTTGATCAAACAACAAAACCTCCAAGGAGTAGAGGATTTGGATTCGTAGAATTTTCAAATGCTGCGGATGCTGCTAAAGCTCAAGCTGAGATGGATGGTGTAGAGGTTTGGGGGAGAATGATTAAGGTCGATTTTGCTAAAGAAAACCCTAACAGAGTCCCTAGATCTGAATATCAACATGAATCAAAGGTAAATGCTTTTATGGGAGATGATGATGACGTTACTTTCTAGTGCGGTTTTCAATACTCTACATATCTAAGAAATCTGAGTGGTATGCTCAGATTTTTTTTGAAAGAAAAAAAAGAAAAAGCTGACTTTTTTCCGTTGTTTTGTTTGGAATTGTGTGTTTTTTGAGTAAAAAGCAGAAAGTGGTCCAAGAATATGATATTTTTCTATCAAAAAGCAGAAAGTGCTCCAAGAATATGATATTTTTCTATCAAAAAGTAGAAAGTGACCCAAGAATATGATATTTTTCTATCAAAAAGCGGAAAGTGGTCCAAGAATATGATATTTTTCTATCAAAAAGCTCAAAGCGCTCTCAGCCTCTGATAGCATCTTTCTCGTAATTGGAAAAGCTCTAGCAAAAGTGATAGGTTTTGGGAAGCTCAGGATTGATAATCGAAAAAACCTGACGATTCATCAGGTTCTTTCAGATTTTTAGAGCTATACTTATGATTGTTTAGAGTTTTTCGATCGAGATTTGTTTTGCAAAGTCAAATCGGGCTGGATTTTTGACTTCGATAACGAATTTTTTATTGCCAAACTCTTTGAGGAGGTAATTATCACCAGGAAGGGTAAAGTCTTTTTTGAAACTACATTCGTAGATGATCACGCTTGGAGAGGACTGCAGATTTTCCTCGTCGGTATATTGGATCACTCTGGTTGCATAATTACAGCGAACCCCTGCCTGTCCAAAATCTTCGTCTGCACTATCTATAGAATATGCAATATTTGGAGATGGAAAGACTATCGTATATTCTCCTCTGCTTGAACTTAAGCTCATTGTCTTTTCAAGGTTAACAGGAAACTGTTTTACATTAGGGGTTGTTGGGAGTTTATTTGCGTTTGAAGTTTCCTTGGAGGGATTTGTTGGCTCTTCCTTTTTTGGCTCGACTTTTGTTTCGCTCTTGCTGGTGGTTGTTTCGTTATTTTTGGAACCAGTTTCGGTTGCTTCTGCTGTTTGAGCGGTTTCTTTGACTTTGAGATCAAGGAATTTGAGTTTCGTTGGTTTGCTGAGGTCGAGCATTGCTTCGCAGGTAAAGGTTTTTTCTCCCTTTCCTTCCATTGTGATCTTCAAGCCATCGCTGGTTTTGGTTGCGGTATGGTTGGTGATGCTTTCGGTAGTTGCGTCTGCTCCTAGGCAGGTTTTTACTCCGTATTGATTTACAACGTTTTTGATGAGTTCTGCATTAGCGATAATGATCAGATTTTTGATCTTTTCTACTTCTTCGTCCTCTGCATTGTTGATGAAGTAGCCTCGTCGGTTTCCGTTTTGAAAGAATCGGGTTTTCTCTTCAGGGAGCTTGTAAAAGGTATCGCCATTAAGGGTTGTAACTTTTTTGGCAGATAGACCTTCAAAGGTTCTTGTGAGTTCTTTACAATTGGTATCGCCTTGACTAGTGCAAGTGAAATAATTGATGGTGGTAATTTTCTCGTTATCGAGGTTTTTGATGCTGATTTTACTATTTTCTCCCGCTTCGCCTACGAAAGCATATTGATCGAAGAAATTAGATGGAAATCAGATCCCTGCTTGGTTTAGATAAACGCCAGGATTTGTGGTTTCTTCGCTGTTCTCGGAAGGAGAATCTGTTTCGCTTTTACTACTATTATTCTTTTCGATCGTTTTTACCTCTACGAGAGCTTGTCCTTGGTAGATGGCTTCGATTGTTCCTTCAATCTTGAAAATATCCCCTGTCATAATAGTATAATTATTGAGCGAGATTGTTGAACTTTTGAGGAGGAATTTTTCTCCATCAGGCGTCGTTAACGTATGTGTATAGGTCAGCAAATCTCCATCTGCAGTCAGTTTTCCTTCTTTTTCGATGGGGTTTCCCACGGTATAGCCGTTTTCTACTGCGGGGGTAGGAAGGGAGGGATTGAAGAGTTTATTCTTCATAAGGTATCCGCCTCCTCAAATCAAAAGGATAATCACAATTCGGATCCAAGCTCAGCTTTTTTTCTGTTGAGGGTTCCTGATGACTCCTCCAGAAAAATCTGCTTTTTTGGGCTGTTTACTAAAAAAATCTTGTTTTTGCATGGTTATGGTCAAGGTAGGGAATAAAAACGCTTGTATAATAAAAAAAGAAGACTGATTTTCAAGGTCAGATTTTCTTTTTCTCCCCATTTTTTGATGATGGAGGATGTGGTTTGTTGGGCGTTTTTTTGCTGGGATTGAGTATTTAAAAAAATCAACCGGCTTTTTGATTTCCGGTTGATTTGATGGCTATGAGCGGTAGGATGAGGATTAATCCTTTTTATTTTTGTAGATAGTGCGTAAAGCCTCCTCGATAAATGGCACATTGAGCACTAAAGTAAGAATTGCTGTTAGAATAATCATTGATCCGAATCCCTTGAAAATACTGATTCCATAAGCGAAGAGCAAAACTCAGATCAAGAGGGTAGAAACTTGACCATCTCTGATGGCAGATCGGCTTCTTTTTTGTGCGGTTTCAATAGCGGCACTATAAGATTTTCAGCTCTTTACTTCTTCGTTCATTCTTTCAAAGATGAGGATATTCGCATCGACTGCCATACCAATAGAAAGGATAATAGCAGCGATTCCACTGAGTGATAATGCGTAATCAATAAGCTTGAGTGTAGCTAAGAGCACTATAAAAAAGAAGAACATCGTTGCTGTTGTTACGGCGGCTTTTCTTCGTCCGTAGATGAAGAACATGTAAATAATGATAGCGAGTGTTCCTACTCCAGTAGCGATTAATGCTCCCTGAAATGCTGAGTCTCCTAGCGAAGGGCTAACTTTTTCCTCTTGGAGAAGTATGAGTGGAGCTGGCATATTTCAAGCATTGAGTTCTTTTGCCATTGCGATTGCTTCATCTTTTCCAAATCCTCCAGAGATCTGGGCTTTCCCATCGCAGATTTTTTCACGGATGTTTGCTTGGGTTGCAAGTGCTCCTCCTACGAAGATTGCCATACTTTTTCCTACGTTGTTTTGAGTAACTTGGCAGAAAAGCTCTCCTCCTTTGGTATTAAAACCAATACTCACTCCTGCCTGTCCGAATTGTGTGGTTTCAGGTCTTGCGATGGTAAAGTAAGCTCCATTGAGTGGGGTTCCGTCGTTTCCCTGTGCGATTTTTCGAGAGAGTCTGTCTTGAATGAAGATTTCATCAATAGTGTAGAGGGTTTGATTACCTGAGGAGTTGTTTCCTGTGTTTGCGATAGCTGAGATTTCGTTTTTAGCTGTTACACGGAAGACGAGGAATCCGTCAATTTCTATAGGCACCTCTTCTCCTGAACCATTTTGGTAAGATTCTCTCAAATATGCTCCCTCTACGACGTTGGAAATTTGGTTGAGTGGGATGGTATTTAGAAGTTCACTATTGTCTTTGTAAATCTCTGGAAGCTGCTCTAAAGTTCAGGTAATCGTTCTTGCTGCGATATTTTCTGCACCTTGATTTGCATATAATTCTATCATCTTTGTAGGATTGGCGAGAACTTCGGTTCTGATTTTTTCGGCAAGTGCCTTTCTCTGGGCAATAGTCTCCTCAGTTACAGCGTCTTCATTTTGGAGTCTGAATTCAAGTTCTACAGTTTTTCCGATAATTTCTTTTGCTTGATCGAGATCTGCAACTCCTCCAATTTCAACGACTACTTGAGTCTCATCACCGATTGTTTGTGTTTTTGCTACGTAGTCACTTACTCCAAGTCCTGAAATTCTAGAGTCAATGTTTTTTAGAATGATCTGTTCGATATCCTTTTTGACCTGGAGGAATTCATTGCTTGTATACAGTTTTTCGTATTTTGAATAGTCGATCTTGTAGGTTAGTTTTGTCCCTCAAGAGATATCGAGTCCTTTTCTAAAATGAGTAATTCCTTTTGTGGTAAGGATCCAATCCCTTTTAAAGGTTTCAGAATTAAAGGTCCTTCCTACAAAAAATACAAAAAAAAGACTGATCACAAATAGGACTGCGAGTGTGAGTCTTTTTGTCGTCAAAAACCCTTTCATATGCCAATAAAATGTAAAATAAAACGGCGTTAATATATGGTTTTTCTCTAAAAATACAAGACTAACTTGACTTTTAGCTTGCATTGGAAGAAAAGAAATATACTATGCACTGTGATTTAGTTTGTTTGTATTTTCATGATTCCTGAGTTTCTGTTTTTTTTCTTTCCTATGTCTAAGCTACAGATGCTGCTCTTTCTCGTAAGCATTGCTTTTATGATCTTTTCACTAAAGACATATAAGAGAAAAAAAATCTGATTTTTTACTTTTTGTTTTCTTTTTTTAGGTTCTCTTGCGGTTGGAGTTTTTTCACTAAAGATTCAGCGACTTAATGCGATAGGAATTACCTTTGGGCTTAATAGGGGAGCGGATTTGATCGTTTATCTCTCTGTAATGGTACTTTTTTATTTGATTTTTTATCTTTTAAATACGGTTTCTAAGTCGTGAACCGATCTTACAAGATTGATTTCTGCAATGGCGATTGAAAAGGCTTTTCTTGAGAATAAGGCACAAATTCTTGCACGAAAAAATAGTTCTCCTTGGGATGAGTTCGTTTTTAATGTTAGAGTATTTAATGAGGAAAGAGTATTGTCTCAGGCTCTTGATGAAATGATTGAGTTTGGAATTAGAAAATTTGTCTTTATCAATGATGGATCAAAGGATAACTCTCTTTCAATTTTAGAAGAAAAAAGGAGGGAACATCCAGATTTACTTTTTATTATACTTTCCCATACGATCAATAGAGGTTGAGGAGCTGCGAATAAGACCTGATATGCATTTATCAGGAAATATGCTGAACTTCTGCAGGTTAAATGGATGGTTGGGTTTGATCCTGATGGACAGATGGATATCAAGGATATGTTGGCTTTTCAAAAAGCTATTCTCAAGCATCCTGAAGCTGATCTTTTTTTGGGTTCGAGATTTATTAAGTGAGCGAAAACACTTGCGATGCCTAGAATGAGGAGGTGTATTTTGGCTATTTCTAGATTGGTTACAAGGATCTTTTATGGGGTTCGTGTTTCAGATCCGCATAATGGATATAGGGTTTTTACTATCCCTGCATTACAACAGATTACTTTAGAAGCGGATGGTATGCATTATGCTAACGAGCTGAATGAGCAAATTTGAATTCATAAGCTCAAGTTTGTAGAAGTCCCTGTTTATATTCGTTATACTCAATACTCTCTTTCAAAAGGGCAAAAAAACAGCAACAGTATTAAACTGGGCTTTGAAATGCTTTATAAGAAGTTTTTGGGATAATTAGTATTTAAAATCTGATTTTATTCTTATTTTTTAGAGCAATGATTGAGAGTCTTCGCCATTCATTTATGTTTGCTCGGTCGAGTTTGACGAGCTTTGTTGCTGAGCAACCTTTTTTGTTTGTTATTATTGCTTTATTGGTCCTTTGGTCGATTATTTCTCATTTTATAAAATCTGCTCTGAAGAAAAAATATATTGACCCTTATGAACGTTCTAAAAATACCCAAAATAAGCAGTTTTATATTGAGTATTACAGAAGAAGTCAGTATGTTAATGTATTTGGGGTTGCTCTTGTTGTAATTCTGTTTTTTACGTATCTTTTGACTAAAGATAAGATCGTATGAACGGTATTGGCAGTTGGAATTTGAGCGATATTGATTACCTTTCAGACGTTTACAGTTTCCTTTTTTACTTATTTTATTCTAGTGAGGAACTATAATGTTGGAGATACCGTTAGTGTAGGAGCTTGAAAGTATCAGTGAGAAATTTTGTATATTAAATCGCTCTATATGGGGATCTCTGGGAAAAATGATTTTGGTGAAAATACGGGAGAGTTTTATATCATTCCTAATCATCAAATTTGGACCAATCCGGTTGTTAAGGTTGATCTTTCTCTAGATAATGTGACTAAACATTCCCTTACTTTAGTGTATGATGGTACAAATTTTTTGCTTCCTTTTGATGAATTTATTCAAGAATTAGAGGCTTTTCTTCTTGGATTGATGCCAATTAAAGCCGCATCTCAGGTTTCTTATTTTAAAAGTTATATAGGAGTCCGTTATAAGATTGATTTTACTTATGACAAAGATGGAAAACCTTGTGTTTGGATCTGATTTGTGGCAAAGAGGTCTGAATGAGTTCGTATTAAGAAAGCAATTCTTAGCTTCGTTGAGGCAAAAAAAATCTCTTGATCCAGTATTGTCTGATAGCGTAGTTTAAAAGTTTTTTTATTTTCTCCATTGGAAATAGCATTGTTCGTTGCCATTGAAGAGTTTTTTTTCGTTTCGCCTTTCTTCTTTTGTTTTATTAATAGAATAGGTGCTGATCCATCCTCAGAAGATATTTCACTCAAAAGTATCGTTTAATCTCGTGTATATGTTCTCTAAATTCTGATTTTTGATTCTTTTCCCATAAGGAGGATTGGTGATGATTCGAGTGTTTTCTTCTGTGTGAAAGTTGCTCGTTAAAAAGTTTTTTTGCTGAAAAGAGATGATATCTCCTACATTGGCACGTTTTGCATTGTTTTGTGCAATATTTATCATTTGAGTGTCGATATCCGAGGCAAAGATTGTATATTTGTTTTTGAAGATTCACGCTTTGGCATCTTGCTTGATTTTTTCAAAAGTTCAGGCTTCGAAATTTTTGAACTTCTCAAAAGCAAAGTTTCTCCGTGATCATGGTGCGATATTTTTGGCAAGGAGTGCTCCTTCTATAGCGATGGTTCCAGATCAGCAAAAGGGATCGATTAATGGGCTTTTGAATTTTCGGTTGGCGAGTAGAAGTAGAGCAACCGCGAGATTCTCTTTTAAGGGTGCTTCTCAGGCTTGGGTTTTGTAGCCTCTTTGGTAAAGTGCTCTTCATGAGCTGTTTAGATAAAGGTGAGCTTTGTTGTTTTCAATACTCAAGAGGAGCTCAGTGGTAAAGCTTTCTTCTTTTCAAAAATTGGCTATACTTTCAAGAAGTGCTTTATGTGCTACAGATTGGATTGCTCTGCTTGCACTTAGCATTGAGTTTTTTGTCTGGATCTTGAGTGATAATTTTGTATTGCTAAGGTATTGTCCGTAGGCGCTTCATTTTATCAGATTGAAAAGCTGATTGAAGTCTTTTGCCTCTCCGGAAGCAAGTTCGATGAAAACTTTATTTGCTAATCTTGACCAAAAATTAATTCTCATCATATTATACATATCAGTCGTGAGAAAAGTTCCTGTTTGAAAGGTATCTCTTGGAGTTACTCAGATTTTTTTTAGTTCGTTTGCAAGTAAACTTCAAAGTCCAAATGGACATGTGATTAGGAGATTCATATGCTCTTTAGAAAAAATAAAGCAGATTAACTTATATTTTTTTATATGAAAGTTTCAAGTTCTTCTCCCTATTGGAATTTATCGGGGGGAGTATTTCTCTTGATTTAAGAACTAGTTTTTTTATCATTCTTTTATTTTTTTGTTAAACTTTGGAGTTTCGGTTTAGATCTAGGGAAGGTTGGTATTTTTGTATTCTTTATATTGGTTTATATGTTTTTTTATTTTCTTAGGTGGTGCTATGCATATAGAGTATGAGTTAAAAGTTTTGGATATTGATGTGGAGCAGGTGAGGGAAAAGTTATCAAATCTAGGAGCTTCTTATTTGGGAAAAAAGTTTTTTCGCAGATATACCTATGATATGCATCCACCTCAACCAGCGAAATGGATTAGGTTAAGGACGGATGGATCTGTTTCGACGTTAACCTGTAAACATATTCTTGATCCAGATGCAATAGATGGTGTAAAGGAATGGGAGTTTGAGGTCTGAGATTTTGTCGTTGCGAATCAGTTTCTTGAGCAAATAGGATATAAGGCAAAGAGCTATCAAGAAAATATGCGTGAGAGTTATGTATTTATGAATTGTACAGTGGAAATAGATTCTCGACCTTTGATTCCAAGTTATCTTGAGATTGAGGGGAATTCTGAAGAAGAAGTGCTCAAGGTTTTGCAGGTTTTGGGGCTTGATCGTTCTGATCATACCTCCCAAAATACTATTCAGATTTATGAGAGATATGGAATTTGTGGTATGGATCAATTTCCTTATCTTGCTTTTGATTGTGTGATAAAAAAGTAGGAGTTTGATGTTATGATTTTTGAAATTTCGTTTGTTGAGTATTTTTTACTAAAAAGGTAATTCTAGATTGATTTTTCTTCTGAATAAAAAATCTCTTGCATTTTTTGATAGCATCTATATACTTTAGGTGCTACGGGGGAGAAAAAGAAAAATAAAAAATCTGACTTTTCTGGGCATATAATCTGAACTATTCAGACTTTTATTTTGTTTACATACGTATTAAAATGGCAAAAATTATTGGAATTGATCTTGGAACGACCAACTCTTGTGTATCCTATATGCTCGGTGATAAGTCCGAAGTGATCGCAAATGCAGAAGGAAATAGGACTACGCCTTCTATCGTGTATATCAAGGGGGATGAATTGCTTGTTGGAGATTTGGCAAAGAGAAAGGCGATTTTGGAGCCAAAAAATGTTGTATATGAGGTGAAAAGATGGATCGGTAGAAAATATTCTGAGGTAAAAAATGAACTTGCTAATATGGCTTATGAAACTAAAGAAGGAAGTGATGGAGGAATCTTAATCGTTGTTGATGGAAAAGAATATAAACCTGAACAGATTTCTGCATTCATTCTCCAAAAACTCAGATCTGATGCGGAGAAATTCCTTGGAGAAACGATTACTCAGGCGGTGATTACTGTTCCTGCTTACTTCAACGACTCTCAGAGAAATGCAACCAAGGCTGCTGGAGAAATTGCAGGGCTCAAGGTAGAAAGAATCATCAACGAACCGACTGCTGCGGCTCTTGCTTATGGAGAAGGGAAGAAAGCTGATGAAAAGATCGTCGTATTCGATCTTGGAGGAGGAACTTTTGATGTAACGGTGCTTGACATCGGATCAGAAGGGACTTTCCAGGTACTCTCTACTTCTGGAGATACGCAGCTTGGAGGTGCAGATTGGGATCAAAGAATTATTGATTATGTTGTTGATGCTTTCAAAACCAAGGAAGGAATCGATCTCAAAGGAAATGCAATGGCAATGCAGAGAATTAAAGATGAGGCTGAAAAAGCAAAAAAGCAGCTTTCCTCTGTAGAAAGAGTTGATATCTCTATTCCGTTTATTACGACAGGAACCGACGGACTTCCTAGGAATCTTGATGAATCGTTGACTAGGGCTCAATTTGAGAATATTACTTCAGATTTGTTTACAAGATGTAAGGAGCCTGTTTTGAATGCTTTGAGAGATTCAAAATTAGAAAAATCTGAAATCAACGACATCATTTTGGTGGGAGGTTCTACGAGAATGCTCAAAGTAAAGGCAATCGTAAAGGAAATCTTCTGAAAAGAGCCTAAATCTACCGTAAACCCTGATGAATCCGTTGCTCAAGGTGCGGCAATCCAAGGTGGAATCGTGCAAGGAGATGTGAAAGATATTCTTTTGCTTGATGTGACTCCGCTTTCTCTTGCGGTTGAAGTTGAGGGAGGAATCGCTCATGTGATGATCCCGAGAAATACTACTATTCCAGCGAAAAAGTCTAATATCTATACCACAGCTGCGGACAATCAAAACGCCGTAACCGTGCACGTGACGCAAGGAGAAAGACAGTTTGCAAGAGATAATAAATCGCTTGGAATGTTTAATCTTGAAGGCATTCCTCCAATGAGAAGAGGACAGCCACAAATTGAGGTAACCTTTGATATTGATGCGAACGGAATCCTTAAGGTGCATGCTCAGGAAAAATCTACTGGAAAATCTCAGGAAGTGACGATTCAGGGTTCTACCAATATTTCTGATGAAGATATTGCAAAAGCTAAGGCTGATGCAGAAAAATTTGCAGAGGAAGATAGAAAAAGGAAGGAAATTGTGGAATCTAAAAATAAGCTAGAATCCCTTGTTTACCAGATGGAAAACCTCCTTACTGATCAAAAAGATAAGATTTCTGAGGAAGATAAAGAAAAAATCCAAAAGCTGATTACTGATGGAAAGGCAGTGAAAGATAAGGAGGATGCTGAGAAAACTCATATTGATTCTGAAATTGAGAGGATTCAAAGTCAGTTTCAGGAGCTTTATCAAAAATATCAGGTACAACCTGCTCCAGATCAACCTCAAGCTGAAGCTTCTGAAGGAGAAAAACCAGCTGATGGAGAAGTGATTGATGCTGACTAGATTTAGGTAGAAAGTTTAAAGGAAAAAAGAAGCTCTCCTTAATAGCAAGGGGAGTTTTTTCTTTAGCTGAAGTCGGAATATATTTTTTGCTGGGGAAATGCTTCTTGAAATATTGTAATAAAATTTTTAAAGCATGTATCTGGAATCTTGATAAGTCCCTTATCTGTTGGCTGAACGAGATTCCAAAGATCAGAATTTGGATTTTTTTTCTGCATTAATTTGAAATCTCATTGGTTATAAATGAGCGAGGCAATGAATATGGAATTGATTTTTGAGATATTGATTTCTTTTTTAATTTCTTCTAAAAATTCTTGATAGTGTATTTCGTAGTTCTTGACTGGCAAGAGCGGAAGAAATCTGAGTCATACCTTATAACCTTTCTCGAGGAGCGTATTTATGGCTTTTATCCTGGCTTTTAGTGGTGCTGTTCCTTTTTCGTATTTTGAGATGATGTTTTGAGGATTGAGCGAGAATGCAATTTCTGTGTTTTGAGGAGGAGTTCCTTGGTTTGCTTCTAATATCGAGGTAATCATTCAGCTTTTTGTCCTTGTTTCCATCAATGTATTTTCAAACTGTTCCATAAAAGGAAAAAAATTCTGATTAAATTCGGTCAGGTGGTCTAAACCTTGAATATCAGAATAATTACTTGCATAGAAGGTGATTTGTCAGTTATATCCTTGATTTCTGAGCTCTGAGATTGAGCTTTTTATCTGATTTTTTATGTCGTCATAATTTACGAAAATTGTAGGATATGGGCTTTTAAAATTCCCTTTAAGATAGCAATAATCACAGTCAAATACACAATTTAGGCTTGTCTTAAAGAAAAACGATTTCCCTGGATAACCATAATGATTTGGAACAGGAATTAGCTTGGGGTGTTCTTGTTTGGCTAAGATAATAGCTGGCTCTACTTTTTGATTTCCTATGTTTTTATCAAAAATATTCTTGTAATGATCTATTTCTAGAATGTTGGCATGAGATAACTTTTTGAGGATTTTTTTGCTAAGAGGGTAGTCTTTTGCTTTGGATTCGAGATAAATAAGCATCAATGTTTTTGCAAAAGTAAAAAATCTGTAACACAAAAACCCTTTTTTAAAATGTCTCAAAAAGTATTTTTATCTATTGGAAATTGATATAAGCAATCAAAATGAGAACCTGCATTAAGCAATGAGGTTCCCTTCTTGAGAAGCTGATTTTTTTTGATATTCTGCGATTTTCCCTTCGTAGAATTCTTTTGGGTTTGCTCACATTACGCCCTCTTCAAGCTTGTGATTTGTTCCGAAAAAGACTGTTGGTATAGAAACGATACCGTATTCTTCTGCCAATTCTGGAACCTGATCTACATCTACTTTCAGAATTTTTACCTGATTTTCATCATATTGCGTAGAAAGTTCTTCCATAACTGGTGAGAGCATCTTGCATGGACCACATCGTGTTGCAAAAAAGTCAACTACGATTACTCCCTGAAACTCATTAAGAATTTGCTCAAATTCTGCTTTTGAAGCGATGATTTTTGCCATATTGTTTTTTTAATAAAAGGATAAAAGCTGATTATTTAAAGTGGAAGATTGTTTTTAACGAGTCTTTAAACTTGTCATAAACGGCGGAGTTGATGCTATACTTGATATGTTTGCCGTCTCTCTTAGTCACTACGAGTTGAGCTCTCTTGAGCATACTTAAGTGGTGGGAGATTAGGTTTTGAGGCTCTTGGAACTGGTTTACTATTTCAGACACACAGATGCCTCATAGAGCTTTCTCATGGCGTTCTGTCAGGAGGCAGAGAATTTTTAGCCTATGTGGTTCTGCAATTAATTGGAGTAGTAAACTGAATTCCTCAAGGTTCTCAAGTCAAGAAATGCACTTACTCATCTTCTCTCTTTAAATAAAACAACATATATATACTGATTGATGTGTAAAATACAAACCATTTTTACTACATTTTGTATCGCTCGTTACTGATCAGATTTTTTTAGTTTTTAGCTCAAAACTCACATCCCTGATCCTTATAATGGGATACCACTTCAATAAAACTATTTTCAGAAGTGATGCTTTTGCAGATTTCCTGAAGCTGGGGTTCGACCAATAAATGCCGAGGTTCAATAACTTCTGCTAGCTGCTGTTTCATTATTGGCTGATTTTCTACCGGAGCTTTGGAAATAATGCATTGATTGAAGGCTACGATCTGGTTGCATAGTTCTACAGGGAAAATTCATGTTTTTTCACTCGTCTTTGATCAAGTATTCGTTCACGTATTTGTTCACGTATTTTGGGATCACGTGAGTAATGCTGTGTTTCAGGAGTTAAGCTCTTTTGCCATAGCATTCCCAGAGAGAGAGGATGGTAGTTCTCAGGAAAGGGCAACTTCTGTGGATCAGGATCCTATGTTTTCAGCACTAGTGCTGCCTGTAGATATTCAGTCTTTAGGTTTTTTTGTGCCACATGCGGAAAGTAGTATAACAAGTCCTATTATTGAGATGAAGAGGTGTGTTTTTCTCATTTTTTGCTTTGCCTATTAAAAGTTTAAGGTTCTTATTTGCTTTCGCTTTGATTTTCCCTCTTTATCATTTTGAGAGTTCATATCCTTCTTTTGTATCTTTTATTTCCCAGCCAGCTTGCGTAATTTGAGTTCTAAGTTCATCAGCTAAGGCATAATTTTTCTCGGATTTGGCTTGTTTTCTCTGCTCGGCGAGCGAAACGATTGCTTCTGGGACTTCCTGAGATGCGGGAGTTTCCGTAGAAAAGAGTCAGATTTTTAGAACTTTTTCTTCTAATTCGTGTAGAAGCGGTAAAAGCTCACTTCCTGACTTGGAAAGTGCAGAATGCAGCTCAGAAAGGAGTCTTGGGGTATTGAAATTATCAGCAAGGGCTTCATCAAAGCGAAGTTTCCAATCTTCAGTGCTTGCTTGCTGAGAAGCTTGAGTCAGATTTTGGATTTTCTTGATCAAACCTGCTCTTTCATTTTTGGCTTGCTCTAAAGCTTCCCAAGTGAAGTTTTGAAAGTTTCCATACTGAGCCTTAAAATAGAAATATCTCAAATCAAGCGGAGAATAGCCTTTGGCCTTAATATCTTCCAAAGAGTAGATATTTCCGAGTGATTTACTCATTTTCCCTCCATCTACCTGCAAAAATTCGTTGTGCAACCGATACTTCACCCACGGCTTCTTTCAAAATCAGCATTCTGATTGAGCAATCTCGTTTGGATGATGAATCGTGATGTGATCAGCTCCCCCATGATGAATATCAAACTGTTCACCAAGATATTTACTAGACATCGCACTACATTCTATATGCCAACCCGGAAAACCAACTCCCCATGGTGAATCTCGCTCCATTTCCCTTTTCTCATCGGTCAGAGAGAATTTCCATAAAGCGAAGTCTGTCGGATTTTTCTTACTTTTCATATCAACACGCTCTCCTGCCCTCAGGTCCTCAAGTCTTTTTTTATAGTTTGGTCCCATGAGTTTCCCATAATCCTCTACTTTACTCGTATCCATATAGATTCCATCACCCGGAACTTCGTAGGTGTAGCCTTTCGCTTCTAGGCTCTGCACAAGTGCGATTTGCTCGGCGATATGCTCAGTCGCTCTTGGCATCACATCAAAAGTCTGAATATTCAGCTCATTCATCGCAGTCAGGAAGATTTCTTCATATTTTTTTGCGACTTCCCAAGCGGAGATTCCGTCTCTTTTCGCTCCTTTTTCAAGCTTATCCTCGCCCTCATCACCATCAGAAACGAGATGCCCTACATCGGTAATATTCATCACCGCTTTCACTGGATAGCCAAAATACTTCAGCACATTCCTGATCAAATCCGCAGTAAAAGTCGCTCTGAGGTTTCCAATATGCGGAGTATAATAGACGGTAGGTCCACAGGAATACATTCCGACAAAATCCTTTTTCTCCCCATGATAGTTAGGATCTTGCATCAGTGGGATAAAGTTTTCTTTCTCACGAGTGAGGGTGTTGTAGAGTTTAATCTGCATTCTTTATGATAAAAAAATAAATTCTGAAAATGTTATCGTTTAGGTTGTTTTTTGAAAGGATATGAATATATATTGTATTTGCAACAAAAAAATAAAAATTTATGAAAAAGACAAAGTTTCCCTTCAATCAGATTTGTCGTTTGCAACTTGAGGTAACTGAGAAGGAACAGCTTCTTGAGAAATTGCTCGCCATTGCTTCTCGTGATGGTGTGGAAAAGCTTCGAGTTGAACTTGAGGAGCTTTCCTGGCAGATTGATCGTATGGAGGAGCAGGGAATAGCTCTTTCAAACTATCCTCTTTCGATGCAAGTTAGTGATCTGATTTCTAGATGTGATCGTTTTATGAAGCAGGGGAGTTGAGTCTTCTACGTGAGACTCATGAGATGGTTAGTGTGTTAATCAAAAAAAGCTAGGCGAATGAGCCTGGCTTTTATTTTTTTCGTCGGGAGTGTATCTTTTGGAATACGTCAGATTTTTTTAGTAGATAAAAAAGCTTGAATAAATAGTATAGTATTGGATTTAGAATAAGATCATAATTCCCGTAAAATTCGATTTTTTCTCCTCCTAAAGATTCTTTGAATCTACTGACTCAGGCAAGGGGATGAGAAGGAAATCCCGTTGGAGCACCTCCCATTAGATCACAATATTCAAATCAATGTTCTTTTGCTCGTTGGAATAATCAATACTTTAAATAGTGGTGTCCTCCTATATTTGCGTATTTCCTGTCAGAGAAACCGTAGAGGTATACGATAGTCTTTTCGTGAAAAATACAGATGCTTCCAGCGATAATTTCGCCATTAAGTTCTGCTACAAAGAAGGTTCCTTGATGATTTTTTTGGAGAGCGTCTAAAAGATTATTGTATTGCTTTCTTGTGATGGTTGAGAAGCCTTTTTCTCCTGCGATGATTTTTCGCTTTTGAAAAAAGGTTTCTTGGTCTTTCGCGGTTCATTCTCTGATTTTTATACCTTTTTTAATGGCTTTTTTGATTTTTTCTGCTGAGCCATTTTGCATTTCATTTAGTAGCTCTTCATCCGTTTTCTTTAGATCAATGATAATTGTTGTTTGTGGCATATTTTCTTTAAATGCTGGTTTGAGATTGGTTTCTTTCGTGATTTTATTGAGGGTATCTTCTCTCATTTCTTTGATTTTTTCTGGGAAATCTTTGTCTTTGAGGTGTTTGTTGTCAAAATGGCTGATTTCGTTGATAAATCCTAATTGGAAAAAGATATTCCCTCGTTGTGATCAGAATTTTCTTTTTAGATTGTTGAGTTCTTCTCTGACTTTTCAAAAGTTTTCAGGCATCTTGATTCCGATGGCTTGAAACTCTTTGAAGGTAAAGGGTCAGATTTTTTTTTCTTTAATGAGATAGAAGGTTTTTTGACCGAAAATTTGCGTGAAATTATGGCTTTTTTGATAGATCTTTGCTTGTAGTGTCTCTCGAATTTGGCTTTGATAGAGTGAATACATAGTTGAGGTGATGAGTTAAAAAAATATCTGCATTAAGATAGGGATTTTGTGCGATAAATCAAACTTAAAAATTCTTAACTATTTTAAATTGAAAGTTGTGAGAGATTTCGTATGATTGAGGAGGAATATTTTTTTATTTTATCTTGAGAGAAAGGTTATGCAAGAGACAGCATTAGTGATTATCAATATGCAAATGGCATGGACAGATAAAGATTCTGAATACTATCTTGGGAATCTTGATGCAATGGTAGAAAAAATGAACTACTTAATTGCCTATGCTCGTGAACTTGGCTATAAAATTATTTTTGTCAAGCATCACGAAACGGAAGGAGTTTTTTCTGAAAAAAGTCCTTTTTCTGATTTTATCCCGGAATTAGCTGTTGAGAAGGATGATATTATTATTCCTAAATATAAGGTAAGTAGCTTTTATCAGACTAAACTTGAGGATGAGTTAACTGGGGTGAAAAATCTGGTTGTTTGTGGGGCTTTGACGAATTTGTGTGTTAGGATGTTTGTAGAGGAGGCTTACGATAGAGAGTATAGGATTGCTTTGATCGACGATTTGACGGTAACTTATTCGGAGGAATTGCATGATACGACATTGCAAGATTTGGTAGATACGAGGTCTGGTTTGACGATTTTGCCTTTGGAAAAGTTTATAGATTAGGTGTTTTATTTAATTATAGAGATGATTATGGAAGGAGCGAAGTTAGTGAGTAGATTGATTGAAAAATATCAACCGAAAACTATTTTAGATTTGGGAGCTGGGAAAGGGTATTTTAGTATTTTGGCCGCGAGTTATGGAGCTCAGGTAGATGCTGTAGAAGATACGAGTATTAGGAATCTTTATCCAGATTATCTTAAGGTTCATCCTAGTGTTTCTTTCTATGAAAGTAAACTTGATGAGTTTGAAATTACCAAAAATTACGATCTTGTTATTGCAAAACATGTTGTGATGTATATGGATAAAAAGTATGTTTTGGAGAGGCTGATTCCTTCGGTTTATGAACATCTCAATAAAGGAGGACTTTTCTTCTTGACCTACCATCATTCGGATTCTGAACTCTTAAAAGAAGAGGCGGGGATTGTACATTATACGCTTGATGATTTTAAATCATTTAAGGGAAGCTTCTCGGTAAAGGATTTTGGGGATTATTCTAATCCTGCTCCTTGAATCAATAAAGAGTATAAAATCTGATTTATTATTTTACAGAGGAATTAATTCTGACTTTTTATTTTTTTGTATGATCGTGATGAGAGAACTGAAAATTCATGGTATTTACAGACATTTTAAGGGAGATCTTTATTTGGTGGAAGATGTCGCTACGCATTCTGAAACGAAAGAAAAATATGTCGTGTATAGGGCTCTTTATGGTGATATGAGTTTGTATATTAGGCCACTTGAGATGTTTCTAAGTGAAGTTGACCATGAAAAATATCCTAATGTTGCTCAAAAATGGAGACTAGAACTTCAAAATATTCCGAGTGTGGCTAAATAAGAAAAATCAGACTTAGCCTTTTTATCTTCTCCTCCCCAAAAAAAACAAAATGCTTCTTGATACGGTTTCTGAATATGATAATGAGAAAAAACATTATCTCCTTGATCTCCGAGAGAGGACGATTTTGGCGATTGCTAATCAATTTGATCATAAAAAAATTCTGACTTTTCTCGGAAAGGTTGGGATTATAGATATTGATGAAACGAAAAAAGAAGTCGTGATCTGAGTTTCTAATGATTTTATTCTTACCCAGATCAAGAAAAACTTCCAAAAAGCGCTCAAAGAAGCCATTATGGCGACTTATAATTCGCAGTTTGGGGTCAGATTTGTGGTCTATCCTCCTTTTGCGAATGCTCAGCACGAGCTTCTTACTGATCTCAAAAAATTGCTCAATATCCAAGAAAGCAAAGCTTCCAAAGCTCCTCAACTTGAAAAAAATCTGAAATCAGAGTTAGGTAATTATTTTGGGATTCTCTTTGAGCCGAGTTATCGTTTTGATACTTTTGTTGCTGGGGCGAATAATCAGTTTGCCTTTTCTGCTGCGAAAGCGGTGGCTGATGCTCCAGGAACGGCATATAATCCGCTCTTTCTCTATGGGAATGTGGGACTGGGGAAGACGCATCTTATGCAAGCGGTAGGAAATGAAATCATGCAGAACTTCCCTGAAAAAGTCGTGATTTATCTGCCAGCTACCAAGCTCGTAGAGGAGATTGTTCAGGCACTGAGGATGAATAAAATGGCAAATCTGATGAAAAAGTTTGATGAGGTGGATGTGCTTTTGATTGATGATATTCAGTTTCTCGCGGACAAGGACAAGACGCAGGAGGTTTTTCACAATCTCTTCAACGATTTTCATATGAAGAAAAAGCAGGTGATTATCTCCTCCGATCGCCCTCCAAAAGAGCTTCTCCATATAGAGCCAAGACTCAAAAGCAGATTCGCACTGGGGTTGGTTGCTGATGTGCAGGCTCCAGATTTTGAGACGAGAATCGCGATCTTGGAGTCAAAACTCCAGTCTAAAGGGATCAGTATTGATTTTGAATATTTGTCCTTGATTGCGCAGTATATCAAGAGTAATGTGAGGGAGCTGGAAGGGGCTTTGAATATTTTGATCACGAGGCAGCAGCTTTCCAAGGCGGAAATTAGTGAGGAAGATGTGTATAGCTGCCTGAAAACGCTCGGCTATTACTGTGATGAAAATCAGATTAACTTTGAGCAAGTCATGCAGCAGAATAAAAAATCCAGTAAAAATTTTTCTACGCTCGTAGAGATGGTTGCCCAGTATTATTCGCTTTCTATCGCAGAACTCAAATCTGACAGCAGGAAAAAGGAAATCACCCAGGCGAGGCAGATTTTGATGCTCCTTGCCAAGAAATATTTCAATCGGACCTTGGAAAAGATCGGTGATTATTTCGGTGGAAAGAACCACGCAACCGCGATCTATGCGATCAATACGATAGAAAAAAAACTGAAGTCTGATTCTGAGATTCAGCATGATTACAGGGTTTTTGTGGATTGGATTGAGCAATAAAGTAATAAAAAAGTCAGATTAGTGTTTCTAGTCTGACTTTTCTCAATGTTCCTGTCTTTTGTCTTAGAGACAGATGATACCTTTTCTGACCATATCTTCCAAGACTTTCAGACTGAGTTCGTTTTCCTTTTCGAGGATTTCTAGGCGACGAAGTCTCCGAGGTCTCTTACAGCTTAGTGTCTCTCGAAATTCATACTGCTCCACGTGAGAAACACCAAACTTTATTACATCTCCCTCTATATCAAAGGAGACCTTCCCTTCTTTCCATTTTACAAAGAGTTGCAATATCTCTCTAGGGCTGGTAGACTGCAAAAGGCGCTCTCGTTCTTCAGAATCAAGTTGACCGATTTTGATTACGAGGTCATTCCAATTGTAACCCTTGCTATGGAGTGTGAGACATATCCTTAGCCGTAGTTTGAATTTCTCCTCTTTATAGGCTTTGTATGCTTCACCTCCTGAGATTGGTTTTGACTCTCCCCCCAATTTTTTATAAATACTTTCAATGTCGTTCATACTATTTTTATATAATTTTTGTTATCCCCTACTTATAGACAAAGTTATAGCAAAAGTCAAGATAATATTTAGAGAAAAAACCCAATTCTTTTATATACTTCTTGAATCTTTTTCTCTGCGATAGCATTTGCTTTTTCTGAGTGTTCGTGAAGCATTTTACTGACCTCATCTTCAGAGATCTCCTTGAATTTCGCTTGGATCGGCTCTAAGGTTTCCCTCATTTTTTCAAAAAGGTAGTCCTTTGCTACTTTATAGGAGAGTCCTCCTGCTTCATATTTTCTCCTTAATTCTTGATCTTCTTCTGGTGTCAAAAAGAGCTTGGTAAGCTGATAAACATTGCATTCATCAGGATTTTTCGGTTCTTCAACGGTTTTTGCGTTGGTTGGAATCTGCTTTATTTTTTTGAGCATACTGTTTTCTTCTTCAAGCAGTCCAATATAGTTGTTGTAAGATTTGGACATTTTTCTTCCATCGGTTCCCATTACGGTTGCGACCTCATCTCTAATCAGAGGAGTTGGTATTTTGAAGGTTTCCCCATAGCGAGTATTGAATTTCTGAGCAATATCTCTGGCGTATTCTACATGTTGTTTCTGATCTTTCCCAACCGGCACAAAATCCGCATCATAGAGCAGAATATCCGCTGCCATCAAAATCGGATAGCAAAAAGTTCCAACGCTGATTTCTCCAGATTTTCCCTTTGCCAAAGCGTCTTTGTAGCTGTGCATTCTCTCCATTGTTCCCATAATCGTCAAGCAGGTTAAAGCCCAGTTGAGTTGTGCATGTCCAGGAATTTCTGCTGGATTGTAAATCAGGGTTTTTGCCATATCAGCACCACAAGCAAGATAGAGCTTTACGATCGTCATACTATTTTTTTTCAAAATTTCCGCCTCTTGCGTCTGTGTAAAACCGTGCATATTTGCGAGAAAAAGAAAAAATTCTGAATCAGTATTTTCATTTTGCATCGTGATAAACGGTTTCAAAGCTCAGAAGTAGTTGCCAAGGTGGAGTTCAGATCAGGTTGGTTTAATTCAGGTAAGGATTCTTTTCATATGAAGTTCTCTAAAATAAAACTTGTTCTTACTGTAGGGAAATAGTGCTTGCTTTCAAGAGAAAATCGATTTTTTGGAGAGCTTTTTGGAGCTTTTAGGTAGAATTGTTGAGTGTTTTTTTGTGAACAGCATCAGATTTTAGGTGTTTTATGATTTCCTGAAAAATCCATTTGCAAAATTATTCCAAAACCATACACTAGGTTCAGACTTTTATTCACTTAATTTTTTTTGTGATGAAAATTAGTTTGGAACTTCCAAAGGTTCTTGGAATGCTCGATATTGCTGCGAGATTTGTTTCTAAAAATTCAACGCTTCCGATCCTTCAGAATATCTATCTTAAGGCATCAATTGATACTTTGCTAATTAGAGCAACGGATATGGAAAAATATGTTGAACTTGAGCTTCCTTGTGCGGTTCAGATTGAAGGAGCTGTAACGGTTAATGCGAAGACTTTCCTTGATATTCTTAAGACGATTGATAGTGAAACGGTGGAGTTTTCTGTTGATCAAAAGACAACAGTAATGACAATTAAAACTTCAAAAGACACTTTTGATATTAATGGAATCCCCGCTTCTGAATATGTTGCTCTTCCAGAAGTTCCTCAGGACAATACTTTAATGCTAGATACGATGATTTTTTCAAAAGGAATCGAGAAAGTTGAGTATGCTATTACAGAAAAGAGCTTTTCACCTGTTTTGACGGGAGTCCTTATGAAATCAAAAACGGAAGACGCACAGCACAAAATTATTTTTGTGGGAACGGATAGTTTTAGGCTCTCTGAATATAAGGTTACAAGTTCTCTTTCTCAGGATTTTTCTCTGATTATCCCTAAAGTTTCTATTAGTGATATTAAATATTTGGTGAACTTTGCTATTGATAAGGAAGTTCCAGAAATGACAGTTAAGTATTCAGATAATTTGATTGCTTTTGAACTTACTTTGTGAGAGGTCAGGATCGTTGCGACGTCTTTGCTTATTCAAGGGAACTTTCCGGAATATGAAAGAGAGGAGGTCATGCCGACTCAATTTTCTACTAAAGTGATGCTTGATAAGAGAGACTGTGAAAATGCCATCAAAAAAATCTGAATTTTGACTAAGGATATCAATAATTTTGTTCAGATTGAAACTCAGGCGACCAAAGTTATTATTTCTTCAGGAAAGACAGATAAAGGAGCGGGAACTACTGAGATTCCTGCAATTATAGATGGAGAAGCACTTACTTTCGCTGTAAATGGAAGATATGTGATGGATTTTATTAGAATGATGTCTTCTGATACCTTGATCTTTAATATTGTGAATGGGCAGAAACCTTTGGTATTAATGGATAAGGACGATCCTTCTAATAGATATGTTGTGAGACCACTTACTAACATTTAATTTCAGATTTTTTTCTATGTTTAGTCAGAAAAAAAAAGGATTTACTTTGTTTGAGCTTCTTATTGTTGTTTTTGTGCTTGCGATTTTGATAGGGAGTGGAATTTATGGGATTTCTACTGCTGGAGCAGACAAGTTTTATGCTGAAAGCTGCGTGAATACTTTATATGGTCCGGTTTCCAACTGGATTTATTCCGCTTCGGTTGGTAAAGTTATTTCGTGAGATGTGGTTCCTGATGCTTATCTTATTAAAGTTAATAGTCAAAAATCTGGTTTTGGACTCTATTATACGTGAAATGCTGGAGAAATAGCGGATAGTGTTTTTTCTTTGTCCGATATTTCTTATTGTCAAAAAGGGGCTAAGTATAGGGTCCAAGCGACTTTTGATTTTTCAGAGATTCGTATGCTGCCCTCACTTCGTCCTTCAGGAATAGAAAATTGATTTACTATTTATTCAGGATTAGATTCTTTAGCGACTTGAGCGATTGTTTTGAAGTTTTGTTCTCCTGCAGCAGAAACAGATTGTCACGACTTTTGAGAGATTGTTTTTGATGCGAGAATTGGAGCAGTAAGAAAGAGATTTTGTAAGCGTTATTTGCCAAAAGATGGATCAACATCAGAAAATGGGAAAACCTGTAAGGAACGAACAACTGGATTCTAGCGATTTTTCTTTTCGTTTCTATCAAGTTATGGGCTATCCTATTCCAAGGTTAAGGAAGTATTTTCGATATTCAAAAAACTGATTTTCTCCTATGAAAACAGACGATTCTTCGTATTTAGAGGAAAGTATCAAAGATCAGGCTTTTTCTTGGGATGAAAAAGGGGAATTGATTCAGGATTTTTTGGCTTTTATCAAGAAATGGTGATGGCTTTACTCAAATTTTCCGTTTGTGGATCAGATTTTTTTGGCAAATAGTTTATGTTTTAATGCGTTGAAAGCTCGTTCTGATATTGATCTTTTTGTTGTTACACAGAAGTGAAGAATTTGGACAGCAAGGTTTTTTATGAGTATTTTGATGTGGTGTTTTGGTATTAAAAGGACGAAAAAATGTCAATTTAAAAGATTTTGTTTGAGTTTTTTCGTTGATGCGGATCATCTTGGTCTTGAGCATTTGCTTTTAGGTGAACAAGATATTTATCTTCCTTATTGGGTTGCACATTTGGTGCCTCTTTATCAGAGAGTGAGGAGTGAGCGTTTTTTTCAAGTGAACTCTCGGATTAGACGCTTTTTACCGAATGCCCCTTTGAAACAGGCTATCTTTCTTGGAAAGTTGAAGGTATTTGAGGGGAAGGGGCGATTTAGGCGTTTAATAGAATGTATTTTTATGGGGAAGCTTTGAGATTTATTAGAAAAGCTTATAAAGCTGATTTGGCTTCCTCGTATCCAAACCTTAAGAGAAAAGAATCCTGATCTTCATCATGGAGTCCTTGTGCAAGATGGGATTTTGAAATTTCACTATGATAAAAGGAAAACTTATTCAGATTTATTTTTTTCTGGTAACTCTTAAGTTAAGAAAAAGGATGTTTTTACACATCCAATCCTTTTACTGATTTTGCATTGGTGAGGATGAAGTGCTTTCTGGATTGTACATCTTCTCCCATCAGGATTCTGAAAAGTCTATCGGTTTCTTCTGCATCTTCTACGGTAATTTGTTTGAGTTGCCTCGTCGCTGGATCCATCGTAGTTTCCCAGAGCTGTTCTGCATTCATTTCTCCGAGACCTTTATATCTCTGGATTTCTACACTATCATCAGCATTAAATCCGTACATGTCCGGCGTATGCTCTTCATTGTAAACATACACCTGCTTTGCTCCTTTACTAAACTTGTAAATTGGAGGACAAGCCGCATAAATATGTCAGCCTTCAACTAATGGTCTCATAAACCTAAAGAAGAAGGTCAAAAGTAGCGTTCTAATATGGGCACCATCAACATCGGCATCGGTCATGATGATGATTTTTTCGTATCTGAGTCCTTCAATATCAAAGCTATCCTTGATTCCGCAGCCAATCGCGGTAATGAGTGCTTTTACCTCATTATTTGCCATCATCTTTTGGATTATCGCTTGTTCGGTATTCAGGATTTTACCTCTGAGCGGAAGAATGGCTTGGAATTTGCTATCACGACCGGATTTCGCACTTCCTCACGCAGAATCTCCCTCCACAATGTAAAGCTCCGTTCCATGTTTTCCTCTTTTGCTGCAGTCAGCAAGTTTTCCTGGAAGCACGCCACCAGCAAGTACATTTTTTCTGAGTACCGTTTCTTTTGCAAATTTTGCAGCAAGTCTGGCTCTCGCAGCGAGGTCAATTTTTGCGAAGATTTTATCAAATTCTTCTGGATTTTCTGCAAAATAGTCTTTGAGATAGTTGTAAGTCAGCTCTTCAACTGCTCCACGAACATAGCTATTTCCGAGTCTTCCTTTGGTCTGTCCTTCAAATTGAGGTTCAGGGATTTTAACCGTGATGATTCCATAGAGACCATCGGTCACATCTGAATACTGAAATTCTCAGATTTTTTTATCTATTTTATCTCTTTCTTTCCCAATTTCATTCATAATCTTCAATAAAGCTGATTTGAAGCCGAGAATGTGAGTTCCTCCATCTCTGGTCGGAATATTGTTTACGAAAGATAAGGTATGGTCGTTATTGGTATTTACAAACTGGAACGAAATTTCTGCTTGGCAATCTTTGGTCTCCGCTTTGAAATAATGAGCAGAACTCAAAATCTGCTGATTCCCAACGAGGTTCCTAAGCCAAGTCTTGATCCCTCCTTCATACATAAATCTGCTGGCAAGTCAGGTTTTCATATTTTTGAAGGTAAAACTTACCCCTGGAGTGAGGTAAGCTGCTTGCTTCATTCTCGCAATTTCCGCGGTTTCACTAAATTCAAGGGTCTCAAAAATTGTCCCATCAGGCATAAAAGTCACTGAAGTTCAGGTTTTGTCAGTTTCTCCAATGATTTGAAGTTCTCCCTGAGGAACTCCTTTTTCAAATCTCATTTTGTGGATTTTCCCGTTCTTGTAAACGGTCACTTCAAGCCAGGTGGAAAGTGCGTTTACTACAGAAGCTCCCACTCCGTGAAGTCCTCCAGAAATCTTGTAGGCTCCTTTGTCAAATTTCCCTCCGGCATGAAGCACAGTATAAACGGTTTCTAAGGCTGATTTTCCAGTCTTTGGATGGATATCAACCGGGATTCCACGTCCATTATCGTGCACTGTCACAGAACCATCTTCGTTGAGAATAGAAGTTACATCTTTACAGTAGCCCGCAAGTGCCTCATCTACTGCATTATCTACGATTTCCTGTATCATATGGTAAAGCCCTCTATAATCCGTGCTTCCGATATACATTCCAGGTCTTTGTCTTACTGGTTCTAGCCCCTCAAGCACTCTAATCTGTGAGGCATCGTATTCATGATTGTTTTCTGGCGTGGTCATTCAGTTTTGCTTTTTTAATTAAAAAAGTAATTTATTCAGCTTTCAGTTTAGGAAAGTCAGAGTAAAAAGCAAGTCAAAATTGCTCTTTCTCGTCAAGGGAAATTGTTGTGTGAGGATTTTTTATTTTCTTATTTTGGAAGATTTTCTTGCTTTTGGGAGAGGAATGGCTAAGATTTATTATGATATTCTTTGCGGAAAGTCTGATTTATCTTTTTCTTTTCTGATGAAAATACGCTCTTGAAAAGGATTTACACTGATAGAAATGTTGATTGTAGTTGCCGTGATTGGAATTTTGACGGCAGTTGGAATGGGGCTCAGTCGGAGTAGCTTGGAAAAACTTAAAGTAAAAGCTGTAAATGAAGAATTTGCCGGTTTTTTTGATACGTTATTTTTGCAGGTAAATGCTTCAAACTATTATCAGTGAAAGCCATTTTCTGGTATCACATTAAAACTTTCTGTATGAGAAAGCCAGATTCCCTATGCTTATTGATTGAGAGAATGAGATGAGTTAAGCTGAACCTTTGCTGGAAAGTTTGAAATTGCTGCATTGAGTGGTGATGGAGAACAGCTTTCTTCTGTTGAGATGCTTTATCTTCCTTTTTCTCCGGAATGCTCTATTTCTCATGAAGGGAAAGCTTTCTCTCACTTGACTTTTTCTATTCGTTCGAGAGGGACTAGTCAGGCTTGTTTTGAAATTGCTCAAGAGTATTGTAAATTAAAGATGATAACTTGTCAATAAGAGGTTATACGGATTTTTAAATTTTTTCTTAAATAGATGCAAAAATCAGATTGTCTTTTCTGTAAGATTGTAGCGTGAGAAATCCCTTCTTATAAGGTTTGGGAAAATGACGAGTTTTATGCGTTTCTTGATCTCTTCCCTAACTGTAAAGGGCAGACTTTGGTGATCCCTAAAGAGCATTTTGACTCAGATTTTTTCTTAATTGAAGATTCGGACTTTGCTCAGCGCTATTTTTTGGCGATTCGTGAAGTTGTTGAACTTTTAAAGTCGAAATTAAATGTGGTGAGAGTCGGGATGATTATGGAAGGAATGGGCGTAAATCATTTGCATATTAAGCTCTATCCAATGTGGGGACTTTCTGAACAGCGAGAGCCTGAAGTTAGTCCTGAAAAGGTTTTCTTTGATCGCTATCCTGGATTTTTGACTACTAAAGTGGGGGATATGGCTTCGCAGGATGAATTAGCAAAACTTGCGGATATTTTCAAGGTATAAAATTCTCTCTTTTGTTTCCGAAATTGATTTTTTCTAGAAAAAGAGGGAAAAATCTGTATACTGATTCAGTTTTTTTATCTTTTTTGATTATTCTGATGTGAAGATGGCATAGTATTGCAGCGAAAAAGGCTTCTGGAGATGCTAATAAATCCAGAATGTATAGTATTATTGGAAAGAAAATTCAAATTGCAGCCAAAAATGGAGCAGATCCAAAGATGAATCCTTCATTGGAGATGATGCTTGAAAAGGCTCGTTATCATGGATTGCCAAAAGATGTTGTGGAAAGGGCGATCTTGAAAGGTTCTGGGCAACTGGAATGAGAAGAAATGAAGGAAGTTTTTTATGAAGGGTATGGGCCTAATGGTTCTGCGATTTTGATTAAAACAGTAACCTCAAATACGAATAGAACGTCTCAATCACTCAGAACTGCCTTACAAAAAGCTTGAGGTTCTATGGCTGAAATTGGTGCTGTAGCTCGACAATTTAAGGAGCAGGGATTTATCGTGATTGATGGGAAATCACAGATGGTAGAAGATAAAGGAAGGCAGATCGAACAAATCACGCCTTTTGATGCAGAAACATTAGAGATGGAGATGATGGATTTGCCAATTGAAGATTTAAGTATTGACTGACAAATAGCTGAGGTATATACTTCTAAATCAGATTTTGTTTCTGTAAGAAAGGCGGTTGTTGAGCTTGGGTATCACATTTCTGAGGCAGATATCCATTTTTTCGCAGAAAATCAGATTTCTCTAACTGGTGAGGATAGAGAAACTTTTGAACGTATTTTGGAGGTTTTACATGATGATGAGGATGTAGATCAAGTTTATCATAATCTTGCTTTATAAGCGAGCTTTCAAACTGTTCTAGTTTGTGCTTCCAATACTTTCCATGATGGAGAATACAACTCCAAAAATTCCTGATGCAACGAGGGCTACGATAACCCCCATGAAAATTAAGATGATAGGCTCTAATATTTTTGAGAATTGAGTAATTGTATTATCGAGTTCTTCTTGATACATTGAAATGATGTTTTGCATTGACTCCTCAAGATGGGCGGTTTCTTCCCCAACTTTGATCATTACTGCGACGTTTGGGTAGATGATCTGAGATTCTTCTTTGATAGAGTCGTAAATGCTTTTCCCGACCTGTAGTCAAGCCATTACTTTTTCAATCATATCTTGGTATGCGGGGATTGCTAGAACACCTCTTAGTAAACGAAAGGTTTGAACGTAGTCTAATCCAGATCCAACCATCAGTTTTGTATATCTTGCTCGTTTGATAAGATAGTAGGTTTTTGTCATTTTTCCTACCAGAGGTAGAGACATCAGCATTTGAGAGAAGTTTTTCTTTCCTTGTTCAGTTGTCCCATAAATTCCGAGTATAAGTCCAATAGTTCCTAGTACTATTAGGATTGTTTTTCGGGAAAGGACAAAGAAATCTGAAGTAGATTTTAGGATGCGAGTGATTAATGGGAGTTTATCCATATCTCCAGTAAACATTTCCAAAATTCCTGGAAGCACAGAAGAGAAGAGAAATAATACTGCAGCAACCGACATAATAATCAAAATAGCAGGGTAGGTGAGTGCTGAGGTATATTTATTTTTGATGGCATTGAGATAGCTGTATTCATTGGCTAGCTCTTGTAATACTATATCTAAGTTTCCTGTATTTTCTCAGGTTTTGATGATTGCACTATCAGATTCATCGAAATATTCGGGTAAACGTCCTATGGCATAAGAAAAACTCTTTCCCTCATTGAGATGGTGGGCAATGCTTGTTGTGATTGTTTTTAGTGCGTAGTTTGTTGTGGATTTTTTGATCGTATCAATCGCTTGGAGGATTGAAATCCCTCCACGTAGCATGTAAACGATTTCTTTGTAAAATAAAATTTTATCTTTTGCAGAGAATGAATTTTTATCCAAACCATGCATTGTGGCGATCACAATTTTTTCAAACAGACCTCATTCAATTTTTTCTGATTTTTTTTCTGTTGTCATGGGGAATTATACGTTAGTTAAAGTAGTATGCTGTTAAAACTATATCAGTAGTCTGACTTTCATCATAGGCCATCATGTCGTAGGAAAGTTCCTCAATCTTATATAGAATACTGTCTTCGGTATTGGAAATGATGAATTTTTCAATGTTATCAACAAAAGAAATCAGATCATCTTTGTCAGAAAACGTTACTTTGATAAGAATTGGTACTTTCATAAATACAGAATCTTTTTCAACAGGTTCTGGTTCTCCTATTTCGATACCCTCGATGACTCCGTTTGGACTAGATACCTTTTCTCTTGGATTATTTTTTATAAGGTATTGGTCGAGATTTTTGAGAACTTTCTTTTCGTCGATTTTCATTTTTTTCGAATTTAGATTTCCCATTTGCAAGTAGGAAACTACGCTTCAAAAGTCAGACTTTAAACCTTCTGATAGTGACGAACATTCCTTTTCTTCGTTTACACAAGCTATTATTTGATCTTTATTATTTTGCAGATCCTCTAGCTGAACTTTTGATTGTTCCAGGTTTGCTATTTCTGCTTTTAGGTCATTAAGCTCTTTATTTTTTTTCTCTTCTAGAGCTTGAGTTGCCTCAAAATATTGGTAGGCCTGTGGGAGATATCTTGTCCCAAGCAAAACTCATAAAATAAGCAAAATAATGACATATACTTTATATTTTGCGGTTACGACTCTTGTAATGCTTAATTGGTTTTCACTGGTATCTTCTGAGAGTTCTTTTATATATGCTGTGTTTTTTTGCTGCTCAAGCAGATTATTGATTAATTGTGGATTCTGTTCTTGCATCGTTTATGACTTTAGCAGATAAAGTGAATTTAAATCATCTGGTATCTTCGTTTCTTTCATAATTTCTTACTGCGATATCGGAGAGGAATTCTAACTGATTGAATTCGTCCAAAACAGAGAATCCTGATCCTCCAGGTGCTAAGTATAAGGCTTTTAAGTTTCCTACGACTCCATTCAGAGAGAGTTCTTGAAGGTTGATTTTGAAGTCAGATAGGAGGATATTTCCTCTTCCTTCTTCTACGTGTTTTACTTTGGATAGGATAGAAAGGATTTTTTCTATGTGATCACTTCGTGGTAGGTGATTTCTTGTTGTTTCTAGTTTTTTCACAGCGAGAAATTCTTTTAATCCAGTATTTTGATAGAAGTTTTCGATTTTTTTCTGTTCATTCTGGATTTCTTGCTCTTTAGCTGCTATTGTTTGCTCTTGCAAATATGAAAATCCATAGAAATATCCAAGAGCTCCTAATCATCAAATAAAGATAATGAGAAGTAGATAATAGCGGGTTTTCCAAGCTTTTGTCGCTTTTGATAAACTCATGTTTTCTGATGCAAAAGATATAAAACCTGACATAATTGTAGCGTTTTTTTTATATATTGCAAATTTTGGCGATTAATTCTTTTGTGTAATACTTTTTTTCTTCCTCTACGCCTATCTTCTTTTTCTGATAACTATTTGCTAGCTTTGTTGATTTTTCTGATAACTATTGTATATCACGAACTTTTAAAATCCTCTTGTCTTTTTCTGTGGTATTCCTAAAGTGGTGGTCGTAGAAAAATTTTATTTTATATCCAGATGCTGATGAGAGATATCAAAGCGGTTTTCGATATTGGGAATGATATGATTAAGGCGGTTGTTTTTTGAAAGGATAATGAGAAAGACATTATTCTTGCTAAACAGATGGAACCTGCACTTGGACTAAAAAAAGGAAAAATCCTTGATGCAGAAGCCTTTACTACGACGATTAACAAGATTATTGAGCATTTTATCAAGAAGCTAGGGTGAGATTTTATTGAAGAAGTTGTTGTTGGTGTTTCTCATCCTGAGATGCTTGTGCATAGGATTATTGAAGGGAAGAGAATTATGCAAGATGAAGTAGAAAATGATGATATTGATCATCTTTCCAGAATTGTTTCTGAGATTTCAATGAAAAATAATTATGAAACCATTAAAATTGCTCCAGTTTGCTGGATTATTGATGAGGATAAAAGAGAAAAAGATCCTATTGGTCTGAAATGTAAAAAACTTGAATTAATGGCCGATGTCTTTATGCTTCCTAAGAATTTTTATACAGGGCTTGTAGATGCTTTTGATAAGGTTGGACTATCAATCTCAGATATTATCCCTAATATTATTGCTGCTACAGAAGTTGCTATAGATTATGATCATAAAGATCTTGGAACCGTGCTTGTGGATATTGGAAAGAATCAGACTTCTTATGTGATTTATGAGGATGGTTATCCGCTTGGTTATGGGACGATTCCTATGGGAGGAGAAGATGTTACGAAAGATATTTCTATTGGAATGCAGGTAGATATTAAGGAAGCTGAAGAAATCAAGAAAACTCATGGTATGGCGGTTCTTTCTGAGGGGATTAAGTCTGATAGTCAGCTTGATCTAGTCTTTCTCTCTGATATTATTAATGCAAGATATGAGGAAATCTTCCTTAAAGTCAATCAGCATTTAGAAAGAATAGAAAAAGACTGAAGATTGCCAGGGGGAATTCTTTTGATTGGTGGTGGAAGTAAGATGATGAATATTGACTATCTTGCAAAGAATGTTTTCAAACTTGCGACGTTCTTTGGAAAGGATCTCATGGTGAATGTTGGTGATCTGTCCCTTAATATCCAGTTTGTTAATTTACTTGGAGTTTATTATCGATCTAATAAGTATGTTGATATTTCTTCAAAGAAAATGTCCTTTAGAGGAGCTGGAAAAGCTTTTAGTAAGATCGGAAACTTCTTTAAGGATCTCTTTTAGTCAATAAAAAACTCAAATATTAATTCAGATTTTTCTTTATATCTTATACTATCTAACTAATGGTTATCAATGAAATTATGCCTGAGTTTATTCCAGGAGCAAAGATTAAAGTCTTTGGAGTAGGGGGGTGCGGAAATAAAGCCCTCAATAGAATGATCCAAGAAGGATTGACAGGAGTAGAGTTTGTTGCGGTAAATACTGATGCACAGGATCTTGCAACCAACCTTGCACAGCAGAAAGTAAATATCGGACTCAATATTACGAAAGGACTCGGAGCTGGAGCAAACCCTGAAATTGGAAGAAAATCAGCGGAAGAAGATGAAATGAACCTCAAATCAGTAATGCAAGATACTGATATGGTTTTCATTACTGCTGGAATGGGGGGGGGAACTGGAACGGGAGCCGCTCCAGTTGTTGCAAGTATCGCAAAAAGTATGGGAATCCTTACGGTTGGTATTGTTACGAAGCCTTTTAGCTTTGAAGGAAAGAAAAGGTCTATCAATGCTGAAGAAGGACTTAAAAAGATGAAAGAAGTTGTAGATACCTTGATTGTAATTCCAAATGATAAAATTTATACTTTGGTAGACAAAAAAACGACCTTCAAACAAGCCTTTACGATGATTGATAAGGTGCTTTATCTTGGAGTTCAGGGTATTTCTGATTTGATTACCAAGCCTGGAGATATCAATATTGACTTTGCAGATATTAGAGAAGTGATGCTTAATTCTGGAAACGCTTTGCTCGGAATCGGATACGGAGCTGGAGAAAAGAGGGCAGTGGATGCAGCGAGAAAAGCTATTGAAAATCCACTCCTTGAAACCAATCTTGATGGAGCGAAGAAAGTGATTTTTGCGGTATCTGGAGGAATGGATTTGACACCGATTGAAGTGCAGGAAGCTGCTGCGATTGTTGAGGAAATCCTTGATGATGATGTGAATATGATTTGGGGAATGAGCTTTGATGAATCTTTTGAAGACGAAGTAAAGGTTACGATTATTGCGACCGGATTTGAAGAGCAGAGCAAAGAACCTATTTTGAAGCCTGTTCAGAGAAATTTCCTTGGTCAAAAGATTGAAACTCCTAAAAGAGAATCTGAAAATTATATCACGAGAGGACTTAGAAACACTGAAACTAAACCAGAACCAGAAACTGTAAAAGAACCTGAGGAAGATACTGAAGTTCCAGCGTTCTTGAGAAGACAAATGAAACAGCAAGGATAATACTTAATGGGAGCTGCCATCGCAAGGCGAAGCCGTGGCGATCTAGGAGAAGGTCGTTCTGGATTGCTTCGCTAACGCTCGCAATGAACACGATCACTCACAAAAAAGATCAGATTTTTTGCTTTTTCATTACTGAACGACTATACTTAGGCGAGTTTATCTTCTTAATGTTAAGAATTATGCAACTTACTATAGCTACGACACAAAATACGACTGTTTATGAGGGGACTATAGACCAGGTGAATTTGCCAACTGAAAATGGGACTTTAGCAGTTTTCCAAAATCATATTCCCTCGGTGGTGAAACTGGTTCCTGGAGTGATTCAGATTTCTTCTGGTGGGAAAATAAGTAAGAGTCTGAGCATTTCCAAAGGGATTGCTTTGGTGGATGGAAGTATGATTAAGATTACTGTTTCTGTGGCTACCATGCAACCTTTGGCGAAACTGGTGGAGCTTCGTGGAAATCAACAACTTCTTGAACTGAAATTACAGAAAGTGAAAAGCTTTGGAAGTGTGGAGGAGATTAGTCAGCTGATTTGTGAGTTAGAGAAAGTTAAAGCTGATATTAAACTCGCAGAACTTTATCATTAAAAAAAAGTCTGATCTCAATGGTCAGATTTTTTTATCTTGCAATACTTTAAAGGATAACTATGCTTTAGTTGGTTTTATTTTTTACTTGTGCAATAATGAAAAGAATTTTACCTTTGTTATTGGTAGTGTTTTTACTTGCTGGATGTTCATGAAAACCAATAGAACTTGTTTGTCCTCCAAGGCAGCCAGAAAAGGTTTATAGCTATGAGGAAAAATTAAAAGGAACTTGATTTCCTTTGGGATTTAAGTCATGCAATCTTAAAGAGCTTTGCAAATACAAGGATTGCACGGTGATGGAGAATCCAAAAGGGGTCTGTGATAACGGAAGAGAGTTTTGACACCATAATGTTGATGAATTGAAAAATGATAATCTGCGTTTAGATTATGGTGAAGGATTGAGTTTTGATCTTTCGTATTTGGAAATCTATTCTGGAGTAAAAGTAAAAATTACTGTTTTAACAGGAATGGTAGAAGTAAATACTGAAGCTCATTATCGCACAAAAACTAAATCTTCATGTACTAGCGATAGTATCGCACCATTAAATGATAAACTATATTCGGGAATCGTTTTTGATAACAGAAATGAGTATCCTCCAATTTGAATCATGTATCCGCCACTTACAAATCAAAAAATTATTCAAGCAGGGGAGTCAGCTATAATATCTGAAGAAGATGTGGTGGTGCTTTCAAATCTAAATGATCCAGAAGCTCAATATTGGGGATTTGATATGAAAATTAGTCCACTAGCCAAAGAAAATCGTATTGCTTATGAAATCATCATTCCAAAAGATTAAATAGAAGCAAAAAAAAGAAAGTCTGATTGCAAATTCAGGCTTTTTCTATATATACAATTGCATTTATTCCTTTTTGTTTGTTATGTATTTCGCGATTCTCTGAAAAAATAAAGAGCTTAGCTTGAAAGAGCTTGAATACGCACAACTAACCAAGCTGCAATCCTGAACTACTCAGCAGATTGTGCTTTTTGAGAATTGTGAGGAGGCTAAGCTTGCCCAACTCGCTGGGATTATCAAGTGGGGAAAGCTGATCGATGGTGATTTGTCAGACTTTTTTTCTTCTTGTGAAAAGAGAATCCTCGGAGTAGCAGATAAAAATCAGGGAATCAATCTCAAAAAACGTTATGGACTCAAGAGATTTAAGCAGGTCGATCTTTTTCATACGGATTTGGAGGTTAAGCAAAAGGGGGTCGAACTCATAAAAATCTGATCGCAGTGGGGACAGGTGTTGGGTTATCAGCAGATTAAACTTTATGAGGTGGTGGATTTTGAAAAGCCAGGTCGTAGTATGCAAATGGGGATGATGCCTGCGAAATTAACGCATACTTTGATGAATATCGGGCTTTCTCTTTCAAAATCACAGGAGAAACCGCTGATTTATGATCCTTTTGCTGGAAGTGGAACGACTGGTTTTTTGGCAAATTATTTTGCTTATGATTTCTTAGGTTCAGATTTGAAACTTACCTTTGCAAATCAGAATAAACCTTGGTGGCTCGCTTCTAAATGGTCAAAACCTGATCAGATTTTTGATTTTTTCTTACATAACGCTACAAAAAGCTTTGAAAATCCTGAGCTTTTTGCTGGAAAGACTCCGATTATTATTACTGAAGGGTGGCTTTGACCTGTAATCAAGGCGAGCACAACGGCAGAGGAAGTCAAGGAATATCAAAGATGGGTGAAAAATGTTTATCGTCCTTGGATTGAACAAATGGCGAATGCTTTCCCTAAGAAACCGACGATGGTTTTTACTATTCCTCGGTATTTGGGCTATGAGAATTTGCTAGAAAAGGCAATTATTGAAGTAGCTGAGCAGGTGGGCTTTCGTTTTGGTTCGCTTCAAGAGCTTTACAAAAGAGAGCAACATAAAGTAGCAAGAAAAGTTATCTTTCTGACGGGGGAGAAGTAAGTTTTTTATCGTTGGAGTAGGTGGGCTTTATCAGAGTCGGAAGTTTTTAGCTTGATGCGAGGAGAATCAGAGGGAGAAACTCTGATTTTTTTCCTTTTTCTTTGTATTTAAGAAGGAGAAAGAAGTGAATTTGCTATTATAAGACTGCCTCTATGCTTGACTTTTATGAAAAAAGTAGAAAAATGTGATTTCTGCTTGCAATTGGTGTGTAGAAATGTATAGTTCTGATACTTAAATAAAAAAAACATATTAAAAAAATGGTAAAAAGATATGGGTTTTAAAACAATTCGTGAGTTAACGCTAACTTTTGGTCTTACTCACAAAAAAGGTATTCAACCTTTTTCCGCTATGGAGTGCTTTTATGCACTTAAGGCGATGTTTGATCGCAAACATCTGATGTTCGGTTCGTTTTTTCTTCCTCTTCTCCTTCTATTGCCTCTCAAGGCGAAGGGTCAGAATTCAGTTTCTGGCACTACTGGAAGGGCTCGGGAGGAAATAACGACAAGCTCTGCCGCTGCTTCTCAGCAGAAGGCGGATGCACCTTCCTCCACTGTTTCAGACAAGACTTCTGTTCGTTCTAAGTGCTTAAGCCCTGAGGAATGTATGAAGATTGTTCAGGAGGACGCCTATAAAAGGAATCCCGAGCTTCGGGAATATGATCTTGCTCAAGCAGAGAAGAAAAAATCAGAAGCTCAACAAGGGATGACTCAGAAGAAAGCTTCTCGGTCTGCTGTTCAGAAAGTTTCTGCTCCGGTTGCAGGCAAAAAGTCTGTAGCTCGGAAGAAGCCTACCCCAGCGGAAAAGAGAAAGTCTCAAGCTCAACAACGGCCAAGCCACAAGAAAGCTCCTCAGCCTTCTCTTCGGAAGGGGGCTGATCCAGGCAGAGAAAAAATGACTCTTGCTCGGCAGCATTCTCTTCCGGCACAGGCGAAGAAGTCTGATGCTGGACAAGGAAGGTCTTCGGGAAAAGATTCGAAGCTTCTTGCTCAACGTCAATCAAGCTCTTCAAAGAGGACTAAATCTACAACCCGCAAGTCTCCCTCTCGTCCAAAAGCAAAAAATCTTCAGGTGCGGGAAGGGATTCTTCAATCAAGGGTGATTCAGGCTTTGGATCCTCCTGCCTTTCCTCCTATTGATCCGTCGTTGCCGAAGGTCTTTGCGAGGATTGAATCCTCTTCACGAGGTGCTAGAGTGTCGGCTGATGTGCATGAATCACTTGTTCAGGCTGGTCAGATAGAGCTTGTTGTCTGTGATGGTCAATCTGGGGTTTTGGTGTTCTATGGTGAAATGGTTGAAGGGGTCACTTCTCCTGATGGTTATGTCCGCTTTGAGGTTACTTGTCCTGCTTCGTGCTTCAAAAACGAGGAAGTCTACCTCTTTAGAGTGAATCAGTGGGGTCCTTTTGGAGGGCGTCAACTGACGGGCTTCCCGAGTTCGGTCTATTTCAAGGAGAATGATCCAGATGCTTCTGTTCATGTGGAGAAAACACCGGTTAAGCATCAAACCACCCTGCGCATTGTTCCGGTTGGTGATGGTGCGAAGTTCGTTGTCAGGACTACGGAGAAACCTAGGCGTGATGAACGTATCTACATCCAGGTTTATGATCCTTTTGATAACTTTTATGCCATTCTCCCTATGCAACTTGACTCAAAGCATGGGGATTTGGCGGTCTATACCTTCTTGGAGAAGGATATGTCCCAGTATCTTTCGGTGCCGAGGGAACGGAGATTCCGTGTATATCTCGGGGAGCTGCCTCTTTTCGAAGAGACTCCGACCGGTAGACAACTGTCTGGTTGTAAAACAGATTTAAGTCATTTTCCCTTTTATCATAATTATGGAGAGCTTGTTCTTTCCGCGTGGTAGAATGTGTTGATGACTTCTTTAGAAGCTCCCTTGGGTTTTCCCGGGGAGCTTTTTCTTTTTGCTGGGAAAGGGGTGGATTACTGTTTGCTCGAGGAGGAAGAGAAGAAGGAAGTCTGATTTTTTTTCTGTTTTTCCCTTGCAATTGAGGAAAGAATCCTTATTTATTTGCTTGCCTCTCACTCTGTGCCATAGTTCTTTTCCTTTTCATGAGAGGTCGCGTATTTTTGGTTGTAGCTCCCCGGGTTTTTTACCTGGGGGGCTTTTCCGTTTCGGGGAAAAAAGTCAGATTTTTCTGTTTTTCTCTTGCAAATGAGGAGGATATCCGTATCTTAGTGGCACTTCTGTTAAGTGGAATTAATGTCTTTAAGTGCGCTGGTCGTGAGACTCGCAATGCTTCTTAGAAGCTCTCCAGGGTTTTTCCTTGGGGGCTTTTTCGTATTGGGAGGAAGGGATTAGATTTTCCTGTTTTTCTCTTGCAAATCGGGAGGAGATCCGTATCTTAGGGGTACTTCTATCATGTCTATTTAGGAATTTTAAGTGTGCTGGTCGTGAGACTCGCAGTACTTCTTAGAAGCTCTCCAGGGTTTTTCTCTGGGGGCTTTTTCGTATCGGGGGAAGGGATCAGATTTTCCTGTTTTTCTCTTGCAAATCGGGAGGAGATCCGTATCTTGGGGCTGTTTTCATTTGTTAAAAGTAAAGTGTTGTGGGCAAACTAGTCGTGAGACTGGTAAGCTCCTTAAAAGTTCCCTAGAGTTTTTCTCTGGGGGCTTTTTCGTTTTGAGGAGAAAGTGTGGATTTTTGGATTTTGTCTTGCTTTTTGGGGAGGAATCCCTACCTCTAGAGTAGAGAACGAAGTTTAAAGGTGAAGAGCAATCGTGGTAATGGGACTAAATTCCCGCTGCCTTCAGAATTGCCCTCCCGACTTCCACTCAACCCCTCAGGCACTTCGTGCCAGCTCCTCTTGACCTCCACTTCGTTTCGTCGAGGACTCCCTGTGGTCGCCTCTTGGGGCGGCAAAAAATAGATGAGGGGATTCGCTTTGGCTCGTTGGTTTTGGGAGGAGCGGGGTGTGAAGTTTATGGGGGACGGGAAGCTTGACCCCCAAATTAGGGTGTCGGACAGGTCGATGATGCAAGAATGACCCCCAAATTTGACTTCCGAGCTGTCGGGAAGGTGAAAATGACCCCCAAATTTGATGCCCGGGGTGATCGGAAGGGTAAAACGACCCCCCAAATTCGATGCCCGGGGTACTGGATTGCCACGCTTCGCTCGCAATGACGGGAGAGGAAGGACTGGATTGCTTCGCTTCGCTCGCAATGACGGGGAGAGGGGGGACTGGAGAAAGTAAAACATTGAAAAAGTTTGAAAAAAATTGCTTTATTTCTCTTTGGGAATATACTTAGTTGAGTTTTTAGCTGAGTAATGAATCTAAAAATGAAGAAAAGTAAATGGCTTCCAGCAGGTTTTTGGGCTGATTTTGATACGACGTTTTCACGTCTTTTTACAGGAGCGAACTTGGTTGCCCTCGGGTTTTCTGGGCTTTACTGTGCGATCTCTTGGATCTTGGAGAGGATATATTCGTCTCTTTTTTCTGGATTGCTAGGGGTTTATTCTATTATCGGGAATGGAAGTCTCAAAGGTGAGCTTTTGCAGCGTTGGCTACCCGCAGGAGATGCTTTTTTCCCCAATATGTGGTATCTTTCAGTGATTGGGCTTGCTGTCGTGTCCTTGAGTTTCGTCATTGCACAGCTTGTGAATAAGAGAAAGGGGAATTTGACCAGATTTTCGTTCTGGTTGATGCTGTTTTTCTTTGTGCTGTTGGCATTACCGTATGTTACGATGTTTTGCAACGGGATCGGTTACTTTTTGCAGCAGTAGTCTGGAATCCGACCCCCCAAGTGAAAGAAAAAAGTGCCACGAGGAAACTGTGGGCACTTTTTGATTTGGGAATGCAGGGGGAGGGGAGCTCCCCCTAGGAGCAAGCGATGCTCAGTTTTGGTCCTCGTAGAGGTTGATGAGGCAGTGGGTGCCTTTTTCGTTTGCCACTTGTGCTTGCACGTCTTCAAGAGAATCTCTCCACGTGAGCGTATCTTCTGTAGCCGGCGGGAACTGGAGGTTATTTTGATCCAGGGAGGGATTACGCCGGACTCTTTGACAAGATTCTATATAGAGTCCGATGATGACTAATGAAATCAACACGAGCATTCCGAGGTTTTTGGTTTTGGCTTTCATAATTGTTTTATATTTTTGGGGTTTATTTACGGTGAGAGGATAGTGATTTGCTCGTTTTTTTCAAGGGAAATCGCGGGAGAGAAGGTGGATTTGATGAAAAAAAGTGCCACTAGAAAGCTAAGGGCACTTTGATTGTTGATGAGGGGGACTGGTATCCTCCTCGGGAACTTGTAAGCTACTCCATCTGTTAGCAGGGGAGCAACGCAAAGGCAGGATGTTTTACCATTCTGCTCGAAATTCGAAGATCTGTCTGTCAAAAAGAGGACGTTTTTCTCTCATTCTTTCTACGACCTCTTTCATCAAGCTGTCCGGACTAAAGAAGTCTTGATCTTTCTCCAAGGCAAGCTTGGGGTCTTTTTCCTTGATCGGGATCTCCCATTCGTCGACTTTTCGCTCGCCTTCAAGGCTGGAGCCAGATTTTACTTTTGGGGGCATGAGCATAGCGGCTATTACACAAGCTATTGCCAGCATTCCTACAAATTCACCTTTTGTCTCTTTCTTTTTGTTCATTTTTTTTAAATTTTATTGGTTTATTGACGATGACAGGATAGTGATTTGCTCGTTTTTTTCAAGGAGAAAAGCAGAAAAAGTCTGATGAAGTCGCGCGGTTTAGTGTGGAGGAGAGGATGGATTGTCACGCTTTACTCGCAATTGCGATCAAATTGTCTTTCTGAGTGAAGCGGAGTAGAATGAAGAATATAGAGTATGTTATTTTATGTCCTTTCCGATGATGTCGGAATGCTTCGCTCTTCGAGCTTCGCTATTATTATATTCAGGAAGAGATTTTTTTTGAGCTTTACTAGCGGTTTTGACTCAGGAAGGTAGTTTGCGGAGGGAGGCAGAGGGAGAAATAAAGGAAAATTAAAGGAAATTTGCATTATTTACTTTTAGGACTATACTTGATGGAGTTTGAATTGATTAAAGGATTCTCCGATATTGGTCTCTGTCGATAGGTATTTATCAAATTTTTATTCGTTTGTAGCGTGTCGGCTATGTGTTGTGCTAATTGGTTTCTTAACCTTCCATTTTTCTTTTATTATTTCAGTGTTTCATGAAAATATATATAAAAACTCCCTTAATTGGAAGAAGAAAGGCGATCCCTCATATGCTTGAGCATTGTATTGCTAAAAGTCGTTTTTGATCATCTGATTTTTTTACTTTTTCTTATGGTAGTGAGGCACAGAGTTATTCTAATTTTACTCTTTATGAATATGATAAATGGCTTTCTTTTGATTCTATTTTCAAGTATCTAACAGATCCTATTTCTAAAGAAGCTTTTGAGTATGAATTGCCTATAATTCAAGAAGAGTGTGAAGATTCAACTTATGATCAAAGAATTTATCAGGAAAGTTTGAGCTTTTTATTAAGTCAAAAGTTTGATTTGAACTCTGCTTCTGGACTTTCTTGGGAAGAGGTACAAAAATATCATGAAAAATATTATAAACTTGAAAATTTTCTTGTTTGTGATGATTCACTTCCTGATTATAGACTTCTTTTTCAAGGAGAAAAATTGAAAAATTTAAGAAGTTGAGCGGCTTCTCCTTTACAGCTCTTTCCTTTTTCGTTTGAAGAAGATGTGTATTTTATTTATTGAAAGCAATATTGTCTTCAGGAGGATTATTGGAAACTCTTTTTTCTTCAAACACTGTTGGAGTGATATATTTTTTTTCAGAAAAGATGGAAATGAAGAGAATATTATCATGGGACTCCTTATTTTTTTACTTATCAAGAATCAAATTGGTTAGTTGTTCCTGATTATGATTTTAACAACTACTCTCAAGATTTTTTCTTACAGGGAAAGCAATATTTATTGTTAATGTTAAAGGCTTGATATTTTTCTACAGTGTTTTTTCTTAATAATTATTTTTATTGAATCCCTGCAACGAGAGGTGAGGTAGTTGCTTTGTGTGAAGCTTTTTCTTGGAGTGATTTTCAAAAAGAAGTTCTGATACCTCTTAATGAAATGAAGAAAGGAAAATAAGGTCAGTTTTTTCTTTTATTTTCAAACTTTTTTTAGCTTTTATGCTATTTATCAATGAAAAAAGTTTTGTTTGTCGTTGTGAATGTTGGGTTTGAATGTGATGAGTTTTCGATTCCTTATGAGGCAGTTGAAAAATGCGAGTTATGAAGTGAAAACTCCTTCAGGACAAGGAGGTAAGTGTAGAGGTGTTTTTATAAAGTCAATTCAAGATTCTCAAATATTTTGATATGATTTTTTTAATTTTTTGATGTTTACTGTATAGAAATTTGTGGTGTTTTTTCAGGAGGAAGGAGGCTGTGTGTTATGATTTTATGAAAAAATGCCCCTAGAGAAGCTAAGGGCATTTTAATTTTGTGTGAGGTTTTTTGTTCTTTTCTCTCACGCTTCGCTAGCATTGCGAATGATTGGCGGGGTTTCCGCTTGGAAAGGTGCAATAATTTCGAATTTTACAGAGCGTAGTCGTGGGTCTCTTTCAGGGTTTGTCCATTGCTTGTTGTTTCGATGGTTACCCTGATTGTTTTTCCGTCCGGTAAGGTGTCTCTGGAGGTGGTTCTCCTCCGAACACCCCAGTCTCTGTCTTGTTCGATTTCTTCTTCGGTGCGGATGATCGCTCTTTGTTCTTCGATCTGCCTGCGCATTTCGTATTGCATCGTCAAGACGCCGCTTATGACCATCCAGATGCCACTTATGACCATCCAGATGCCACTGATCGTAGGAAGCCAGGAATTGTTTTTGAAGATTGCTTTCATGATGCTGAAGTTTTTTTACTTGTCTTTAATTTCAGATTTCCAGAGTTTACTCATCATGATTGAGAGTATCCCTGCGGAGGATATACATACTAGGAATGCAGCTAAACTGAACTGCATATCTCTATTCGCAAAGAACTCAGGAATCCTACTCTGCAATTCGAAATTTTCGGTCAGCAGAGCAATGAAGTTTATTCCAGAGAAGACTGCCAATATTGCTAATATTCCTGCGCATAGATCCAAGAATACTATTAGCATAAATATCCACCTTCGTCGATAAAATTTAGACTCCCATGTTTTGTTTGATTTTCTTGTTCCCATTTTATTCTTCTTTTTTATATTGTTAATTTTTTAATTAGCGGTGTTGACAGTATAGTAATTTGTGATGCTTTTTCAAGGGGAAGGGTGGTAGACTTTAGTAAAGTACTCTATGCAGCTCTTGGAGGTCGGTTATCCCTTCAATAACTTTTAAAATTCCGTCGTGTTGCATCGGGATGAAGCCTTGAGATTTTGCTAGAGGGAGGAGGTTTTCTCATTGCATTCCTTGCATGAGTTTTTCTCTCATGATGTCGTTGATTTCGAGGAGTTCTATGAGAGCAATTCTGCCTTTGTAGCCTGAGTGATTGCAATGTTCACATCCTTTCCCTTTGAGGATTTTTCCTTGATAGTCAGGTAATACGTCTGTTGGGAGGCGTTCCTGGAGTTCAGTCAGGTTTTTTCTTATTTCTGCGTCTTCTTCTGGTGTCGCATCCTGCCATTCGCTACAAAATGGGCAGACTTTACGAACGAGCCTCTGTCCAATGAGTAATTGGAGGGCTGGAGTGAGGAGGTAGGGTTCGATTCCCATATTCATGAGTCTTGAGAGAGAGTCGAGCACTGAATTGGTATGCAAAGTTGTAAAAACTAAGTGTCCTGTAAGAGAAGCATTGATGGCGATTTGAGCGGTTTCTTTGGTTCTGGTTTCTCCAACGAGGATGATGTCCGGGTCTTGTCTAAGGATGGCTTTTAGTCCGGTTTCGTAGTCGTAGTTTTTGTCGTAGTTGATCTGAGATTGTTGGAGTCCTGCGATTTTGTATTCGATAGGATCCTCAAGTGTGATGATTTTTTTGGTTCCGTCGTTGAGTTTTTTGAGAATGGCATAGAGTGTTGTGGTCTTTCCTGATCCGGTTGGTCCGGTGATGATGATGATTCCGCTGGTTTTGTGAAGGTAGTTTTCTAGGACGCTAAGGTTATCTCCCCTGAAGCCGATTTCTCTAAAATCATCGATACTTTCAGTAGCATCAAGGAATCTGATCACGACGCTTTCGGTAATGATTCCTGGCATAAAGCTTACTCTGGCATCGATTTTCTTTAGATTTCCGTCTCTATCTGATGCCTCAAAAGAAAATCTTCAATCCTGAGGGAGATAATCGATATTCATTTTTACCCCTGACATAAATTTGATTTTCTGCATATATTTCCAAAATTCTTGATGAGAAAATTGGAGGATGTGATGAAGGACGCCATCGATTCTTAGTCTTAATACAATTCCGTTTTCTTCGGGCTGGAAGTGTAAATCTGAAGCTCATGCTTGGAAGCTCAGTCTGATGATTGAATTGATGAATTGAGTAGGATCTTGGCTTTCTCTCTGTTCAAATTGTTCTTGCATCAAGGCGACTGCTGATTTCCCTTCAGCTTTTGCTTCTTGTTTGATGAGTTCTGCTCTATGAGCTTCTTGTTCTTCTAAAATTGTATATCGTCCGAGCGCCGTTGAGAAGCCTTCGTTTGTGGTATAGAAGAGTTCATGGTGATAGCCCTTTTGTTCGATCTGATTCAGAAGCTGTTTGACTGCCTCTGGGTGGTTATTGGTCGTAAGAAGCTTGAGTGTTCTTTTTTCCCCTTTCCCGAAAATAAGGAGCTGCGCCTTTTCTGCGGTAGCTTTGGGAATTATGGTCAAAAGAGAGAAGTCTGGATTGAGCCCTTGGATTTCTTCCTGTTCAAGAATAGTTTTTATGAGTCCAGCCATTGAATTGCTTGAGAGTAAATCACTATCAAGTATAGTCGAAACTCGTTATATTTGCAAGAAATTGGAGGTTTATTTCATGGTTTTGATGGTTTGCTGTTCGATTTTGATGAGTGCGGTCGAGAAGGGGAGCTCTAAGAGTTTTGTTTCAAAGTATTTAAAGCCTCCGCTGATGATCATCCCTCCAGTAATGATGAGAAGAATTCCGATACTCTTTTTGAATCGTCAGTCGGGGTTTGCGTATCCTTTTAGTCTTTTTATAGCTTCTCTTCAAAAGAAGATAATGATAAAAAGAATACTTCAGAATCCTAAAACATAGAAGAGAATACTGAGTGTTCCCATAAGAAGATTTTGGGGGAGGATTGTCCCGATGATGAGTGCATAGGTAGGGGAGCAACTCGCGAATATTGGACCTAGAGATGCTCCAAGTGCTATTTCTGCGAGTGGACCAGTTTTATGCTGTTGAGGTGTTTTGACTGTTTTGGAGTGAGGGAAGTGAGATTTTATTCGGTCTCGGAGTCCTGGGAAGAGGAGGAAAATTCCATAGATGGTGATAATCCCTCAAGAGAGGTAGTTCCAAATGTCTTCTGAGAGGTGAATAAAACCTGTTCAGACTTTAAGTAAGAATGTAAAGCTGAAAATACAAATGAGTGCTGATGAAAGGATTAGATAAGATCTTTTTGGATTCGTTTCTGTCGCTGCTCCTCAAAGTAGCACCGGTAACACTGGTAAAACACAAGGAGCTAGTACAGAAAGCACTCCAGAAAAGAAGGCGAAGAATAAGATCATGATTAGTTTTGTAGTAGTTGATAAATTGTTTCATAGTTCCCAGGGTTTTCTCTATGGGTTTCTTGTAAGTTTTCATTTACTGTTATGAAGGTATCCATATTGTTTATATTGTAGGTTGCTCTTAACTCTTTTGCGTTATCGAAATCAATCAAGAATAAGATGCTGCCAGCTGGGATTCCTTTTGTCGTTAATTCATCTTGGAGCACTTTACAACTTCCACATCGATTTGCCTTGAAGAGGAGTCAGATTTTATGTTTTTTTGATAGAGCATTTGCCATTTCTTCGGGGCTATAGTCCAGGATTGTTCAGGTTGTTCATACGGTAGTTTCTGTGCAAGAAGCTGCTGTACAGTTTCCTTGATTCCCTAAAAAGATAAAAGTTCATACTCAAAAAATTAGGAAAAATCAGAGTCCGAATACTAGTTTTTTTGTCATAATTTTGTTCATTATAAATAAAAGCGATGTATCTATATATATTGATATATAAAAATCAAGGTTTTTTTTAGTACATTCTGTAGCCATATTTTTTGATCAGGTTTTTTATCTTTTCTGAGAGCTCTATTTCTACTACGCTTCCAATTTGTGCGAGTTTGTTGATGCTGCTATTGTGTTTGCTCTCTTTGAGGAGGTTTTCTAAGAGTTGAAAATTAAAATTCTTCTTATTCTTTTTGAGTCGTTTTTTGATGAGTTCCTCTCCGTATGCTTTTTGAGATGGAAGGGTGTTATAAAGTGTTTCAAGAATCGCGAGTTCTGGAGTTGCAATGTTGAATTTATAGTTTTCGATACTTACTTTTTGGGTGTTCTTAAAAAAGAAAGGAAACAGATTTTTATTTTTGTTTTTATAGGTTTTTAAAAGAGCTTTCTTTTCAAGAATAATGGTTTCACTTCCCTGTTTTTTGTTGTTTACAAGCAGTATTTCATCAGGGATACTATAAGAGAGGAGTGAAATTTCTAGAGCTTTTAGTCCTCAGATATATCGCTTTCCATCAATGAAATCATTGCAGTGCTTATTGAGAATGCTTCGATAGACTTTGTTTATGAGTTTTTCTTCATCTATTGTATCTTCAGGTTTTTTGACAAGGAAAAGATTTTTTTTGAGATTGATGAGATGTCCTCTGAGTTTGAGATAATAGATAATTTTATACATTTTAGGTTCACTATACTCTTCGTCTAGGATATTTTGAAGCTTTGTTTTTATGACTTCATTAGTGATGATTTTTCCCTGTAATTTTGACAGGTAATTAACAACAGTGTTGAAAGGATTGTTTTTCATTTTACCTTTATTATGCCGATAAATACAAAATAAAATATATTGATTTTTCTAAAAAAATCAAATCGTTTTTTCTATAAAAATTCTTTTGTTTTTTCTCTTGTATTTCTTGTCGAGAATCATACAACCATGATCGTGATAGTTGATGTGAAATTTTATTTTATGCCTCTTTGATCAATGCAAGGTTTTGAAATGCCACTTCTTACAACTTTAGACTTTAATCAAAAAACTGTTTCTGTTGAGGGAATAAAGGCTGTGGTAACTCAAAATTCATCTTCAGATTTTGAAGTTTTGCTTGAAATTAATTCAAATAAGATCCTGATTGCTTTGCAGGGGGCTTATGATGCTATAGAACAGTTTGAAATCTTGGCAGTGAAGGGGTTTGTAGAGTTGAGTGTTTCTTTTATTAATACTATAAAGAAATTAGTAGGACATCTTTTGTGTAGATTAGATTAAAAATAGAAATTTTTTTCTGCTCTTGGCTTGAAAAGAGAAAGACTTTCTTTATCATTGCTGGGAGCTTTTTTTATTTGGAAAGAATTGGTATTTATGTCAGAAACTGAGCTTTTAGTGAAGGAATATTCTAAAAATCCTTTGTGTAATTATGAAATGCAAAATCCTGATGTGACTAGACATGAAGGGAATTTTATTTGTGGTGATGATGTTACGGTTTATCTTAAGATTCAGGAGGGGAAAATAGCCGAATTTAGCTATGATGGGAATCCTAGCACGGTAAGTCTTGCAGCGGCGAGCTTTCTTTCGGAGTTTCTTATTTGAACTTCGTTGGATGAGGTGTTGAGCTGGAATTATGAAACTTTTGTAGAGAAGGGATTTGAAGTTTCTCCTAGGAGGAGGAGAGCGGCCGTTATTGCTTTGCTCGGTGTGAGGAATGCGATCCATGCCTATCGTAATGATGGGATAGAGGAAGATTTTGATGACCTCATAGATTAGGGAAAAGCTCGTAGTTTGCTAAAAATCTCTTGAAAATTGTTGACAATTCTGTAAATTATTTAGACACATGTCTTTTTTAGTCTTCTCAAATCGTTGAAGCGAATGTGGAAAATCGTAAAAGATATCATCCTTTCTGTGCTTATCAATGCGGGGATCCTGTATTTTGTGAGCAGTAATAAATTTGGTCTGGTGATTGAGTCGAAAGCTGATATTTTTCAGGTTTTTTTGCTTTTGGGATTGGTATTCTGGATTGTAAACTTTGGAATTAAGAGGATATTGCATATTATTACCTTTCCGCTAAAGTTTTTGACACTCGGGATTTCTTCAATTGTTATTAATGTAGGAGTTTTCTATTTCTTTCAATGGTTTATTAATGAGAATTGTAGTCAGTTTGCGAGGGTTCAGCTCCCTAGTGGAGATCTTGGATGGGTAAGGGTTTTGATTCTCTCAGTTATTATCTCAACAGTGTATGCTTTACTTAGTAAAATACTAAAATAATGAAAAACGGAATGCGATGGTCTTTGGGGGCGATGCTGCTCTTTTGTGTGCTTGCCTTTATTGGAGTGGTGCAGAATGCAGGGGCGTCAGCAACCTTTAGTTTTCTGGGGATGCGTTCTCTTTCCTTCTTTAAATTGGGAGGGCTCCTGATGATTTGTAGTATCTTGTGAGGAGTGTTTCTTGTGCTCTTCATTCAGAATTTATTTTCTGGTATAAAAAGGCCAGAGCTTACTAAGTTTGATTTGGATAAACCCTTATAGTTTTTATTTTTGAGGAATTAACGGATGAAAAATTATTCAATTACGAGAGAAAAAGCTGCGATTGAGCTTGGAGTTTCTACGAGAACGATTGATAGATATGTGAAAGGTGGAAAACTTTCTTATAAAAAGGTGGCGAATAAGGTTTTGCTTGCGAGAGATGAGGTAGAAGCGTTAAAAGCTGAATTTTCAACCCTTCATAGTCAGGTAACTAGTGAACTTATCCAAGAAGTTAAGCCAAACGTTGCTCCAACGAGTCAGACTTCTCCTGTTGCTCAGCCAGTAGCCGTTAGAGCGGATATTGATCAGGCGATTGAGGAGAAAATCGATAAATTTTTCTTGATTTTCAAGGAGAAGGACAAGATGATTGAGGAAAAAAATAAGATTATCTTTATGCTTCAGCAGAGGATTGGGGAATTGGAGACCAAGATTCAGACGATGATCGCTCTCCCTGATTATAATAGAGAAAAACAAGAAGCTATTATTGAAAAGCAAAAGCTTGAGGAGAGAATCTCCAAACTCAAAGAAGCTGTAAGAACAGAAAAGGTGAAAAATTTTATTATTATGCTGTTTTCTTTGCTGTTTATCGGGATTGCGATTTTCTTGTTGATGAAATATAAGATTATTTCTTAGGGCTGAGGTGCTTTTTAGGCATTCTAAGACCCTAGAATCTCTCATGAAGCAAAGGAATTGGATCGCAACCCTCCAAGCATCACCAGTTCTCCAGGGTTTGAGGACTTTTTGATGCAAGGATTGATGCTGCTGCGGGGTATATGAACTTTTCAATGCAAGCATTGGAAGTTTTTAGGGGTTTATGGACAAAAAAATGCTAGAACTCAAAGAAATTAGGGGTAAATGATCTTTTTTGTGCTAGAATCGTTGGTTTTTGAGGGTAAATGATCATTTGGTCTTCATTTCCGGTCGGTTCTTGGACTAAAATGGACATTTCCTATCAATTTCCATCCGTTTTTTAGACACAAACGGACATTTTGTCTCAATTTCGGGGTAGAAATAGACACAAAAAGTGTTTTTACTCTAAAAAACCGTCGTTTCTGTAGACAGATATAGACATTTTATTGCAATTTCCGATGGTTCTAGTAGACAAAATAGTGATTGTTCTCTGAAAACTGATGGAAATCAGGATTTTAGAACTTTTTTCTGGTAATTTTTTGGGATTTTGAGGATTCTTGGGAATCTGATCTTGCATTTTCTGTTGATTTCTGCATAGTTAGGGCAGTGCTCTTATTCTTGATTATCATCGTATGGAACTCAAAACGCTCCTCAGGACCACCCCACGCTATCTTAACCTCTTGGCACAGAATGGTATCACGACCATTAGGGATTTTTTTAATTATTTTCCGAGAGCCTACGAAGACAGAGCGAATATCAAGCCTCTGAACCAACTGATTTTTAATGATAAAGGTATCACTGCAACTAAAGGAAAAATCCTGAAAAAAACCGTCTTTCGCAGAGGGGGGAGAATGATTTACGATATTAGTTTTGAAGATGAACTAGGGATTCAAGGGACGATTTCGATTTTTAATTCAGGCTATCTTGCCAGCAAGCTCCAAGAGGGATGCCGATACATTATTGTAGGGAAGCCGAGCTTTAAGTATGGGAAAACGATCTTTTCCCACCCTGATATTGTGCCGAGTTCGGATTCTTGAGCGTTTGAGGAAAACTACAACTCGGGAAGGATTTTCCCGGTGTATTCCGAGATGAATGGGATCAAACCTTGATGGTTTGCCAAGAAGATTTGGGAGCTTCGTGATCAGATTCCCGCGGTTTTTGCTGAGTATTTGCCTGAGGAGTTCCTCAAGACCTTCCATTTACTCGATGTGCAGAAAACCATCCTCAATATTCACTATCCTGAGAGCGAATCGTTGAAATCACAGGCGATTCATAGGGTGTTTTTTGATCGTTTGCTAAGAGTTCAGCTTTTTTCTCTGATGAATAGGCAAAGCTATCAAGCTAACAAGAAAACTAGCTCTGAAGCGCAGCAACGGGGGGTGTTGAAGGCGATGCTCCAGCGTCTGCCGTTTGAACTTACGATGGCTCAGAAGAAAGTGATCAAGCAAATCATTGATGATTTTCATAGCGGGAAGCCGATGCTCAGGCTTTTGCAAGGAGATGTAGGGAGCGGGAAAACGATCGTTGCAACGCTCGCGGCTTACTATCTCAAGCAGACTGAACAGGGACAAACGGTATTTCTTGCGCCTCTGGAGGTGCTGGCTCAGCAGCATTATCAAAGTATTGCAAAGCTATTGTTGCCGCTTGGGCTAAGAGTTGAGCTTTTGACGGGGTCGCTGACTAAGGGGCAAAAGGAGAAGATTAAATCGGATCTTCAGCAAGGGAAGATTGATGTTATCATCGGGACGCATGCTTTACTTCAAGAAGGGATCAGTTTTCAGAATTTACAGTATGTGATTATTGATGAACAGCACAAATTTGGCGTAAAGCAGAGAGCGTTTTTCAAAAAGTTTGGAGCACCGCATATTTTGCAGATGTCGGCGACGCCGATTCCGAGGTCGATGGCGATTGCGTTCTTTGGGGAGTTTGATGTGAGTATTATTGATGAATTACCGAAAGGAAGGTTGCCGATCCAGACCAAAATCATCTCTAGCACCGAAGAAAAGAAACTCAAGCCTCGAATCCTTGAGAAAATTAGGAAAAATCAGAAGCTTTTCGTCGTGACGCCGCTTATTCAGGAGAGTGAGAGTCTTGATGAAGTGCAATCTGTCTTGCATGCGTATGAAGAAATTCAGCTGCTTTATCCGGAACTGGAAGGGAAGATTGGTTTACTCCATGGGAAGATGAAACCCAAAGAAAAAGAGGAAGCGATGCAAAATTTTAAGAGTGGGAAGACGATGATTCTCGTTGCTACGACAGTAATTGAAGTAGGAGTAGATGTTCCCGAGGCTACGGTGATGATTATTAAGAATGCTGAGAGATTTGGCTTAGCTCAGCTTCATCAGCTCAGAGGAAGAATCGGAAGATCGGATCTTCAATCGTATTGTTTCCTCCAAACCCCCAAAAAATCGGGGGATAGCTACGAAAGGCTTAGAGCGATGGAACAGACGACGGATGGGTTTAAATTAGCTGAGCTGGATTTACAGAATCGTGGATCTGGGGAGATTTTGGGAACGATGCAGTCTGGAATAAGCGATATTCCGATTGAAATCCTCTCAGATTTGAAGTTTTTGGAGCAGGTGCAGGCTGGGGCATCGTGGCTCTTGCAAAAGTATCCGGATTTGGAGGGATTGCCGAGCTTGCAGAAGTTTTTGCACGAGAAAATTGGTGATATTCTTGCTTAGCTTCGTAAAAGTGGAGGAGTGGAGATTTTTTAAATTCATAGTAGAGGATGTATCTGTATAGTTTCTCCCATTATATTGGGCAGGATAGGGTATTCTGGTGTGAGTTGCCGAGTAAAAAGGTCCAGGCTGGAGATGTCTTGCAATCTGAGTCAGATTTTTTTCTTGTATTAGGAGAGACGGTGAGTTTTCCTGAGCTTTATCAGCTGCGTTATCCGATGCAGGGGGAGTTTCATGTAGCGTTTCCCAAGCTTTTATCAAGTCCTGCACTTGAATTGGTGCATCGGATGGTTTATGAGCGGTATACGACCTATAAAAACACGCTCAAGCTCTTTCTTGACCATGAAATTCAGAATCTGCTTGCTAAAGCAAAGCGTCCTACGAGCAAAAAATACCAGCCTCTTGATTTCAGCATAGGGAAACAGAGGATTCATTCGGAAGAACAGGAACAAATTCTGATTATTTTCCCGGATTTGCGAACGTTTACGAATGTGCTTGATCAGCAAGCTGTCGGCGGACTTTTTCTCAGTGCTTTGGACACTCAAGCCAGGAAAAATCTCAACCGATGGAAAATAAAACAGGGAGAAGAGCAGTTGATCTTTTGCACGGGGGCGGAAGTCTTCCAAGATTTTAAGAAGCTCGGGCAGATTTTCCTTGTTGAGCCACAAAAACGATATTATGCTAGCCAGCAAGACCCGAGATATAAGCTAGAAACGGTTGCGAAAAAGATTGCTGAGCTGCATGGTATCCCTTTACAGTTGATTGAGAGCGAAATGCTCGCTCAATAATCAGATTACTCGGCAGTTCCGCTGCCTTGGAGCATATAGTGCTCGGATAATACTTGTATTGCTTGATCAAAAGAGTCTTCTCCGCTCGGCGCTTTGAGTAATTGGGAGTATAAGTCTGATTTTCCTTGTGGGTCGTCGCTAAATTTGTCGTTGATGATTTTTTCGATGATAGGAATTTGTTTTGCGACCATTCTTGGCAGCAAAGTTTCCTTGCCTTTAATCTCGACTTTTACTAAACCGAATAAAGCTAAAAGCGATGAAAAATCTGATTTAGAACTACTATAAAGATCCCAGAATCCATCATAAAATTTCCCTATCGTTGTATTGATAATTGCTCGATCTGCCTCGCCTACTAACGGCAAAGTCAAACCATTCGTATAATCCACGTTGAAATATACCGCTCCCATAAATTGGTTTTCTTTCAAGAAAACGCTGAGGTTTTTGAGCCGTTGGTTTTTCCTAGCGGTTCAGGTTTCAGAGAGGAATTCCGCTCTTGAGGTGTCGGCTTGATATTTCCCGCCATACCAAACTGCTGTTGTCCCCACTTCATCAATGAAAAGTGGTTTTCCGAGTGCTTTTAGTCTCGTTAGGGTGTTCCATTCTGGATCATTGAGGATTTGTGTTGGAGTTTTTCGTAGTCTGTTGCTCGTTGCCTTGCCCCAATTATAGAAGGTCACCCCGACAATATCTACGTAGGCTTCTCAAGGGTAGTAGTCCTCAAAATGCGGACAGGTGTTGAGGGTTTGGAGGAGTTTTTCCTTTGTTTTGAGGCTGTTTTGTTCTTCAGGAGTCAGTTTTTTTTTCTTTTTTAGTTTGTTGATTTCCTCAAGCACGGGATTTTTATCCTTGCACTGAAGGAGTTTTGCCTGTTGGGAAGGGGTTCAGAGGGTTGGCATATCTCGGTGATTCACAGAGAAGTCAAACAGGATATCTTGCTGATTCAATCCCAGCGAACGTGCTAAATTCCATACATGTATCCATGCTTCTTTGAATTTTGTGGGGTTTGAGCCTCGTGGATACCAACCGCCATTCATTTCATGCATCGTCCTGAAGATTACTTTCAAATTTAGTCTCTTGATTGTCTTGAAAAATAGGGTGTATTGTTCGTCAAAGGCTCCTTTGGCTACCTCTTCAGCAGAAAATTGGTTTGGAGAAAGCGAGATATGATAGATTCTTTGGTTTCCCAGCTCTTTTGCTATTTTCTCCAGCTCAGCTACTACGTCTTGCTTGTCTCGTGGATCGTAAATAAAGCCAATCAAAGGCAGATAGAGGCTATAGTCTTTTTCTATCTTTACTACGTTTTCCACCGTATTATCCGTAGATTTTAGTCCCAGAACGAAAGCAGATGTTGAGAAAGGGAATGCGAGGAAAAATAGCATCAAAATCCAGACTTTTTTCATTTTTTGTGGTAGAGATAAATCAGAGAAACTTATAGTATTTTCCTTATGAAAATCAAGGAAATGGCTTTCTTCTAGCTAAAAATTATGATTATGGTAGGATACTAAAAAGAGTCAAGAACTCTTTTATTTACCAGGGATTTCCCTAAAAAGGATAGCAGTAAAGGTTTTACTCTATCCTTTTCTCTTCATATGGAACTTCTTATTCAAACCCCATTATATCATGAAAATAAATATCTCCCTCACCTTTTAGAGCATTGTGTTCTCTATAGTCAACATGATGAAGAGCTTCTTTTCCTTCCGGATATTTTTGCTTCAACGAGAACAGGATATACCAGTTTCGAGTGGAAAAATATGTCGCTAGAAAAGATTCTTTTCTTTTTAGAAAGACCAGTAGATGAAAATATCTTTTTTTTACAGCAAAAAGTCGTAAAAAATGAGCTTAATAATTCCTCATTTTGACAAAAACTTTATGAAAAAATACTTCAAAAAATACATAAAAACTTTCGTACAAATAGTCTTGATAGTATTTCTTTAGAAGAGCTTTTAAGCTATCAAAAGCAGCGATACCAAAAAAAACATATGCTTCTTCTAGATGAGGAATGAAAGATCATATTTGATCGAGGGAAAGGGGAAACGCTCAAGAAAGGCAAGCTCTCTTTAGATAGTTTTCAGTTCCCCGAAACGCTTGACCTCAGCTATCAAAAAGAACACTATCATCTTTTGCTCACTAAAAATATACAAGCAAGCACGATTTTAGTAGTAGATTTTTTTGGAGCTTATCTTGAGGATATGTTGTACCTCCATGATAGTAAGGCTTGAAAATATTATTATGAACGTTTAGACTATTCCCTTGCAGATTCATTCTTCCTGATTAGTAGAGAAAAAAAGTTTCCCAAAATAAGTAGACAAAGACGAAAACAGTTCTTTGATCTCTTTCAGCCGTATTATTGCGAAAAAATCAGACAAGGAGAAAAAAGAGCCTATATCCCTCAGATAGCACTTTTTACAGAAGAGTATTTCTCAAGAGAAGAACATATCGGTTTAATTTCCCATCTTGATTTTTCTCTCATTTTAGAACTTTTGAAGAGATTTAGAATTATTATGTAGTCCCTTAATTTTTAAACAATTGTATTATAACCCCATACCTTGCACAAGATACATTGTGCAAGTTTTTTAATAAGGTAATTTGCGACTTGTTTTAATACAAAGATTATTCGTTTTAATTAATGGTTTTTAAAAAAGCTGAGATAATTATCCCAGCGTTTGTAAATATTTTTCAGCCTCTAATGCTGCCATACATCCTGTTCCTGCGGAGGTGATAGCTTGTCTAAAGATCCTATCCTGAACATCTCCTGCTGCGAAAACTCCTTCAACGCTTGTTTTGGTAGATCAAGGGATGGTTTTAATATATCCAGTTTCATCAAGTTCTACTTGTCAGCCTAAGAAAGTAGTATTTGGAGTATGACCAATAGCATAAAAAACTCCGGCACATTCAATCAAAACCTTTTCTTGCGTCTTGTTGTTGATTGTCCAAACTCCAGATAAAAGTTTTTCTCCTACAAGTTCAGTAGCTTCTGTATTCCAAAGAATCTGAATTTTTTCGTTTTTAAAGGCTTTTTCTTGCATTGCCTTTGATGCTCTCAATTGATCTCTTCTGACAAGCAAAAGTACTTCGCTTGCAAAGTGCGTCAAATGAATCGCTTCTTCAAGTGCTGCATCACCTCAGCCGATTACGACGATTCTTTTATTTCTAAAGATCGGTAATCCGCCATCGCAGATAGCACAAGCGGAAACTCCCTTTTGCCAAAACTGCTCTTCTCCTGGAAGCCCCATCCTTTTTGCGGTTGCTCCTGTAGCAACAATGAGGCTATCTGCTGTAAACTCTTCATTTCCTACAGTAACTTTAAATGGAGAACTGCTAAGATCTACGGAATCAACAGTTTTTGTGATAATTTCTGCTCAGGCATTGAGAGATTGCTGCCTCATCTGCATCATGAGTTGACTTCCATCAATCCCCTCAGGGAATCCAGGGAAGTTTTCTATCACCGTTGTAGTGGTGAGCTGCCCGCCAGCAGCAATTCCTCCTGCCATAAAACCTTCAAACATGAGTGGCTGCAAAAGCGCCTTCCCCGCATAAATAGCAGCAGTATGTCCCGCAGGACCAGAACCAATAATAATTACTTTACGATGCATTTTACTTTTTACTTAAATAAAAATCTAACAAGACAAGGTATAAAGATATCAATCCCTTTGTCAAGCACAACACATCAATCCAAGAAGATATGTTTCCTTGCTAGTAAAAATTGTTCTAATCTTTTACTGACAAGACATCTTTTCTCATAAAATACAAAAAAAGAAGCAGATAGAACTATCCACTCCTTATTTTCTATAAGGAGGTATAAAAGTATAGTGCCGAAAACCGCTATAACATCTCTTAAACATTATAGTATCTCATGCTTTAGGCTATCCTAAAGATAAGAGTCATGAGAAAGCAACTCCTAAGCATTTTTTATCAAGAAAAGTGTAATCCTTCTCAGAGAAAAGAATCCTGATCTTTCCAAGAGCAGAAAGCGAGATGATAGAGGGGAACCACCCCTTTCAACTTTAGTATCACTTCCAACAATACCCAGAACAAAAAATTCCTCACCAATCCCTTCTAAACCTAGCCCTAGTAAAGCCCTATATACTTTCTTAGCAGAGAGAAGGTAGTAAAGTCGGTTAAGAAACTTTACCTTTCTTACTACTTCAAGCGAAAATATCTAAGGGAATCCTTTCCAGAGGTTATTTAAAAGCTTTTTTCTTTCCTCGAAGTGTTTTACTTCATCGAGTCATAGTCGTTCGCTCTTAACGAAACTTACTAGAGAAAAAAATCTGACTTTTCTTTTTTGCCCTTCATCCTCCAATACAGAAAGATAAAGCTTTATGACTCCTGTTAAACTCAAAAGCCACTGGAAGCTGCCTTTACCACCTATCAACGAATGATAAACGCACTTAACAATTCAAAAGAAAAGTGATTGAAAGAACTTTTTCTATACTTTTATTTTAAAGACCTCATCATAGTTGCTCATCAACAAAGGAGTCTATTTTGCACTCTTTCTTGATGACGATATTATTATAGTCAGTTTTTTCTGAAAATGCAAATTTTTGATGTCTTTTTTCTTTGTTTTTCAAAACTCCTCTTCTCAACCGCTATTTCTTTTATGATTTTTGGGCTGATTTTCTAAGAAAAGGTGATATTCCCACTCAATCACGCTCCAGACTCCCCTCTTTTTTCTGGGCGGGGCTGAGTTTTCCTCTGAGGAGGCTTGGAATTATGCTCACCCTTCATAGAAAGGGAAAGCTTTTCTCTTTCTTTATCAATTTCCAAAACCCTGACCTCTATCTGCTGTCCGACATGCACAACCTCAAGCGGATTGGTTACGAAATAGTCCGCCATCTGCGATTTATGCACGAGCCCGTCATTATGCAACCCAACATCTACAAAGGCACCAAAATCCGTCACATTTCTTACTACGCCTTGAAGTTTCATCCCAATCTCTACATCATCAATGCTCAGAATATCTGATTTGAAGACAGGCTTTTCAATTTCCTCTCTCGGATCTAGTCATGGCCTCTGTAATTCTTTCAAAATATCTTGCATCGTCTCAAATCAAAGTCCGTATTTGTTGGCAAACCCTTCAATTTCTTGTTCGCTGTAGTTTTTGAGGGACTGAAGGGGAAGAATCAGATTTTTCTTTTTGATACCCAACTCCCCTTCCAGAAAACTCAAGGTTGCTGCATAATGATCAGGATGAATCCCCGTCGCATCAAGTGGCTCTTTCCCTTCTTTGATCCTAAGAAATCCTACTGCCTGCTCATATGCCTTAGGTCCAAGCCCTTTCACTTTCTTGATCTGCTTTTTATCGGTAAAAGACCCATTTTCATCTCTATAGGCTACTACATTCTTGGCAACTGAGGCCGATAACCCTGCAATATACTGCAAAAGCGTATAGCTTGCCGTGTTTACATCTACTCCAACTGCGTTTACCACATCTTCTACCTTCTCATTTAGCTTCTCTTTGAGAAATTTCTGATCTACATCGTGCTGATACTGCCCTACTCCGATCGCTTTAGGATCAATTTTGGTCAACTCTGCCAAAGGATCCTGCACTCTGTGAGCAATACTAATCGCTCCTCTAATCGTCACATCAAGCTGTGGATATTCCTGCTGTGCAAGCTCCGACGCCGAATATACTGACGCCCCCGCCTCAGAAGTAATCACATATTGAAGCGTTGGCATATATTTTTTGATAAAATCTGCTACCACTTTCTCCGACTCCCTAGATGCTGTCCCGTTTCCTATCACAATCAGCTGAATCCCATACTTCTGCACCAAATCAAGTAGGGTCTTTTCTGCTTCTGCAATCTTTTTTTGTGGTTCAGTAGGATAAATCACCTCTTTCGCTAAGAATTTCCCCGTTTGATCGACGACCGCAAGCTTGCAACCGGTTCTAAACGCAGGATCAAATCCCAATACCGTCAACCCTTTTACCGGTGGCGTCAAAAGGAGCTGCTTCAAATTATCTCCAAAGACTTTGATTGCAGCCTCGTCCGCTCGCCTCTTTTTGTCGGCTCTTAGCTCTCTTTCTAGTGAAGGCAGGAGTAATCTCTCCAGTCCATCGTCAATTGCTTGCTGTAAATACTCAACCGAGCTGTTCGCAACGCTAGGAATAAAATACTTGCTTGCATACTCCGAGATTTTCTGCTGAGAAAACTGCAATTTGATGCTGAGCTGCTTTTCTTTCTCCGCTCTACTTACCGCCAGATAGGCATAGGAAGGCATTTCAAAAAGTTTCTTCTGATACTGCTGATATACCCTGTATACCCCATTTTCCTCAAAAGTCTTGGTCGCTTTGGTCTCTAATGTGGCGTTTTGTTCTTCAAATTGCTTGATGTCGGCTCTTAAATCTGCGTGATCTGATACCTCTTCTGCAACAATATCCTTTGCTCCCTGAATAGCCTCTGCACGATCCTTCACTGAGGTCTTTTCGTCGCCCGTATCCTTAATAAAGGTATCGGCTCTGCTTTCAAACTCTTCTTTTGTAAGCTCTGCGGTTGATAAGAGCTTTGCTAGCGGCTCCAAGCCCTTCGCTTTGGCGATAGTTGCTTTGGTATTCTTCTTTTCCTTGAAAGGTCTATAAAGATCTTCCACTCTCGCAAGCGTCTCTGCAGCTAAAATCTGATTTTTCAGACTTTCGGTCAAGAGTCCCCTCTCCGCAATCAAACGGATCACGTCTTCCTTCCTTGCCTCCAAATTTTTACGATAACTGTAGAGTTCAAAAAAATCACGAAGCTGTTCGTCGGTAGCCCCCTTTGTCATTTCCTTACGATAACGCGCAATAAACGGTATTGTGTTCCCTTCATCCAAAAGCTGAATAATATTGTTGATTACCGCAGCTTCCATACCAGTTTTCTGCTGTAATTGTTGGAGTAAAGTCGTCATTGCTATGCTCTTTAGTAAAAAATAAAAAAAATCTGATTTTTGACGCTAGTTTGTTCTCGGATTCAAGCAAAGCCTTGGAAATCCGATCCCTGTAAAGCTCTCGCTTGCATCTAAGTAGCAGTGCTGTGGCTTTAGTAAACTGCAACGGGAGCGTTTGGCTCTAAAAGTGAGTAAAAACTTCTGCTATTTCCAAGCATTCTCGAGGGTTTCCTCTGCTAATGTTCTCGATGCTCCTCGATAATCTTATTCTCAAACTTGTCGAGTGCTACGGATATTTGATTCGGCGGGATAACTTTCTTCTGAGCTGAATTTTTCATCACGAGGCTCAGGTCGTCCTTCTTCACCGCCTCCAAAATCGGATTTTCCAGACTTTTAGAGATCTTTTCTTGGGTTGCTTTGGTGTCGGTCATCAGCCAAAAAAACAAAAATAAAGCAACTTCCACTCTAGCGGAATCCTCCCTAATTTCAAGTCAAAAAATGCCTTGCATTTTCGCTAGGGATTGCTAGATATGAGCTGGAAATTAACTATTGACGTGATCTTTAACAGCTTTCCTCTTATAAAAAACAAAAATCTGATCCCTTTTCTCTGTCATTGCAAGCGAAGCGTGGCAATCCAACGACTTCAAACCCGTTATTGTGAGCGAAGTGAAGCAATCCAACGACTTCAAACCGTCATTGCGAACAAAGTGAAGCAATCCACTGACTTCAAACCCGTCATTGCGAGCGAAGCAATGATTTCAAAGGGGCTGTGGAGAAAAAATCCCGCGAATCAAACGTTTCAAGGGGTATATGAACTTTTCAATGCAAGCATTGGAAGTTTTTAGGGGTCTATGGACAAAAAAATGCTAGAACTCAAAGAAATTAGGGGTAAATGATCTTTTTTGTGCTAGAATCGTTGGTTTTTGAGGGTAAATGATCATTTGGTCGCAATTTCCGGTCGGTTCTTGGACTAAAATGGACATTTCCTATCAATTTCCACCCGTTTTTTAGACACAAATGGATATTTTGTCTCAATTTCGGGGTAGAAATGGACACAAAAAGTGTATTTACCCTAAAAAACCGTCGTTTCTGTAGACAGATATAGACATTTTGTTGTAATTTCTGCTCGGAGATGGACATGAAAAGCGTTTTTAGTCTAAAAAATCGTCGGTTTTGGGATCAAAAAGTGCCTCAAAGGATCAGATTTCTTCTTTTTAGATAAAAAAATCTTCCTGAGAAGATGAGCTTTCTTATATACTCATCGTTTTATCTTCTTATGATCAACACAATGACGCATGCAGAAGAAGTAAAAGAGTTTTCTCCCAAGAAACTCACGAATGCCAACTATGGTGCCTTTATGCAAGCCTTTGAAAAGCTTGTAGCGAAGGGGACGACGGAAAAACTTTGACTTGAGGCGAGTGATTTCACCGCTTTTCAAGACAAACTCAAGGTCTTTCTCGATCTCAATCAAGAGATGCACCAAGAAGAATTTACCAAAGCTTTGCAGGAGCTGGACAAAAAACGTGATCAGCTTCTGAGTTTTATCTTTGCTAAGATTAGTATTGAGGCACAGTCCCCTGATACGCAAGTGCAGAAGGCTGGGCAACTTTTACTCCCGCTGAAAAAGCAATATCAAGGGATCCAGACCAAGCAAGACCGTGCAGAGAGCTTTTTGATCAGCGGTTTGCTCGTGGACCTCGAGAAAACCGAGCATGCTGCGGCAGTAGCCAAAATCTGATTGACGGCGGGTGTGCAGGAGCTGAAAACGGTGAATGCCAAGTTTATCGAAACGATGGCAGAACGCTCTGAAAAGGAGATTGTTAGGAATCTGCAAGGAGCGAAGAAACTCCGTGAGGAGTTAGATACGCTCTACAGGCAGTTCATCAGGAAAATTGACGCACTTGGGGTCGTAAGTCCGAATCCAGAGCTGGTGGCGTGCATTGCTTCGCTCAATCAACTCATTCAAGAGACACGTAATGCCCGAACCATCCATAAAGGGTTGGTGGGAGCCTATACGACGGACGGAGAATGGGAAGATGAAGAAAAACCTGAAGCTGAAAGCGTTGCCTCCGAAGCAACAGATAAAGCCTAAGAGGAAGCAAAGAAGGAGTTGGGAGCTATGAGGGTTCCCACTCCTTTTTTAAAGAAAAACGTGTTAGTTTCCGTGAGGCCTATGCCTTCCTTGCCTCCCTTCTCCTCGAAATTGAGGATGCGGGACGGTTTTTTATTCTTATTTGTATAGAAAATGGAGAATCATATTGATCAAATCGGTGTTTATGACAGTGGTCCAGATTTTGGGGGGGTAGGTCCATTCCTCTTTGTGCTCATCCTCATTGCTGTCTGGAATATGCTTATTTTTGGGGTGTTGAGAGCCATAATGAGGATTAAAGATAGTGGAAAAGCTCTCAAGTTGAGAATTATGTGGCTCTCAGTACTTTCTTTTGTTGCCATGATGGGTATTACAGGGGGAAATCAATTACTTCCCACCATAGTAAAGGCCGCTTCTACTAATGAAATACTCCTCTACTGTGGTATTTTTTTGATTTGTGAGGTTTTATCCTTTATCATTATCAGAAAATGGATTATCTCCAAAATGGAAATCTCCTCAATCAAGAAGCTATGGCTTAGCCTTGGACTTGTGCTCCTTAACCTTCCTACCTTCTTTGGAATCGTTTTCTTTATTAATAGTCTACCTTACTTTCACTAAGAAAACTGAGAATAGCTCCCCCATTACCTTTTGGAAAGTGGGGGAAAATTCAGAATTTTAGTTCTTTTTTATAGTAAGAATGAGAGATTTTTTGTAGGATGCTGTAGGTTTTATGTTGTTCGGGGTCGGGATGGGAGAAATATGGGGACTTTTAGCGCTAGCAGGCATCAGTTTTACACGGAACATCCTCATTTTTTTCTTTGAAGCGAAAATTTGCTGAGTGAAAGAGGTTTGAACCTTCCTAAAGAAAAGAATCCTGAAACTTTTCGTGGTTGCCTACCTCGCCTATGCTCTAATACGAGTATGGTTGATGACGCTCCGTGAAATAATCTGGTGATCTCAGACGTTTTTGACCTCACTCTCTCTATTAATCCTTGTTTTTGGGGGGCTTGATCTTCATAGCTATTAATTATCGAGTGCTTTCCAAGAGTGGACTCAGCCCAACTAAGAGAAATTGGTTTGCCCTAGGGATTTCGCTCCTAAGCTTCCCTGAATTTTTTTTGATTTGCCTATTTCTAATGGTGGTTTTGGGGTAAGCTTAGAAAAGCTAAGGAGTTTTTGTGGGAGCTTAGGGGAAAATTAGTATGCTATTTGTTGCATTAGTTGTGCTTTTTGGGTATACTTAGAAAAGCTGATGACTTTTTAGCTGTTGGACGCTATGAAAGTAGCGGAAATTCAGAATTTTAATTCTTTTTTATAGTTGGATGGGAGAAACAATTCTACAACTAATAGCGTATATTGTCGCAATAATGTTTTTATATCCATGGCTTGGATTAGTGGTATTATGAATTATAACTTTCGGATGGAATGCCCTCGTTCTTAGTCTTGTAGCAAAGATTTCCTGAATGCAGGGCATCAAAACCTTACTAAAAAAGAGATTTTTTAAGTTTATCGGAGTTCGATTCTTAGAAAATTTAGGTACACTATGAGCAATGACGATTGTTTGGGGGGTGGCAGATGAAAATCCAGAAGCTTCGATGTGGTTATTCTATGCAATATCCATTGGTATACGAATTCTTATCGCATTTATCAATAAAAATCGGATGCTTTCAGGGTGTGAACTCGATCCGACAAAGAAGAATTGGCTAGCTCTAGGGACGGCTTTCTTGAGTTTCCCAATGCTCTTTCTGATTTTTTTATTTGTAATGGAAACCTTTTTGTAAAATCAATCTTATCTGCAGCCATCATTGTTTTTTATTCTTTTATGGTTTTAGATTAGCTTTTATTGCTTTTATCTTTTACACAAAATGGTATGTTTATAAGTTCTCACGGTCAGCAGTATCTAATTTATGTAATAATATTTATTATATTTATAAAGATCGTAAGTATTATTCGGAATTTCATATTGATATTGATATGCATGAGCAAATTACAAGTTCCTAAACCTAGAAAATTTTTAAAACAAACCGCTTTCAATATGTTTCGTTCTTGGTATTGGGCATGAAATTATGCTTCATTGTTGGCAGGTTGTTTGTTCTTCATTATATCAGAATTTTGTAGTTTAATAGGTATATATAGCTGATGGGCTTGACTCGGGCTAATTTTTGTGTGGTTTATTCCGCTCTTTTTTATGATTGCTTCAGTAGCTTCAGGAATTTTTATTTATTTTTTGAACAAAAAATGGACTTTCTCAAAGACAGAACTCACTTCTGAGCAAAAAGAAAAATTTTGTCGCTATCTTGCATACTTTAGTTTTCCTCATGTCATAATCCCATTACTCGTAATTGCTTATTTCGTTTTTTTATTGCTTCTCTAGCTTTTATTACTTTTATCTTTTATATAAAACAGTATGTTTGAAGAAATCCTCGGCTTATTCTTCGGCTTATTCTTAGGAGAAGCTCCAGATCTTTTAACTTTTATAACGCTACTCTTGGTTGCTATCCTTCGAAATCTCATCCTTCTAAGGATAGTTATCAAAAAAACAACAAAGCCTGAGAATCAAAAAATACTTAAACAAGGAAAAACTCCTGTTTTAGATACTAAAAAGCTTTTTAAGCAGACAGCTTGGAAAAGTATAGGATTTCGGCTCTTAGCAATGGTCATAGCTGTGGGAGCAACATGTTATTCTCCCCTTATTACTAATATGTTATATAACTTCAGTTGACATGAAGAATTTTTTGGTCCTCTGAGATATATAATACGCTGAGCTATTATAACTTTCCCAACAGCTTGCGGAGTTATGATTTATCTCTTCAATAAAAAATGGACATTTTCTGGGACAGAACTCACTCTTGAACAGAAAAAGAAGATTTCTCGTTATCTTGCATATTTTAGTTTTCCTCACGTGATAATCCGACCTCTCGTCGTCCTTAGTATCATGCGGTAGCATTTTGGATTAGTCTTTATTACTTTTATCTTTTACACAAAATGGTATGTTTGAAGAAAATCCTAGTGCAATCTCTGTAAATTATCAATTCTAAGGATTAAGAGCCTTCCCAAGTGGCTCTTTTTTGTTTTTCTTGAAAAAATAAAAAAAAGACCAGGAAGAAATACTTCTGGTCCGAGTGGATACCCGGAACCGATAGCAAGCCCTTAGAATACAGAAGGCTTTTTAGTCTCAATAACGATCGCTATCAAGAGTAGAGAGCCTTATACCTTCTACACACACTATAAACAAAATCGGTTCCAGATACAAAAGGGTAATAAACGTTCATCAGGGTAGATTACTTGATTTATCGCTTCCTCACGCCTTGAGAACGCCTCAGGAGAGAGTAAGTCAGGTTTTTTGTTTAATTTTTATTTTATTGCAACTAGAAGATATGGTTGTGCATCCAAAAAACAAGAAAATTTTTGCATTTCCCTCTAAGATTTAGTTAGCACATTTTTTGATTTTGATGATGTTTTTTGAAAAAATCCCCATAGAAACAGAAAAATCTGATTGAAAAACTTTCGCTCATAGCTATACTCACGGGCGTTTATCCTTCTTGCATCATGAATACCACAAAAACTCTTGGGCTTCTCTTTCTCGGCAGGCTAGAAAAAGAAAAAGGATTTGATTTGATCTATGATTTTATCAGTCAGTATCCGGGCAAGGAGCTTCCTTTTGATCGATACATTTTTGGCAGCGGAAGCTATGAAAGCGGGATATTAGAGCTGAGTTATCATTTCAAGCAGATTCATTTCTTTGGGTGGAAGCCGCTCTCAGAGGTCGAGAGATACCTTGAAAACATAGATTACTGTCTGATGCCGTCCAGATTCCTCGAGACCTTTGGTTTGAGCGCGATCAACATCCTCCAGCAAGGTATTCCGATTGTTGGTTTCAAAAAAGGTGGGCTGATCCCGTTTATCCAAGATGAATATGCAATTGAACAAAGCGAAGGGAGCACAGATCTTGCAAAGTTTTCTAATATGCTCATCAAACTCCAACAAGAAAAAAAGAAAAAGAAATCTGACTTTTACGAACAGTTGGCTCAGCAGTCCAAGGATATTGCAAATCGCTATACCGTAGAAAAACGATATGAACGCTTTCTTTCCCTTACCGTAACCAAAAAACCTCAAAAGATTGTGCTTGTAAGCGACTTTATAAATAAGATTGGAGGGATTGAAACCTATCTCCATGATACCAAGCATCTTTTGCAGCAATACGGTCACCAAGTAAAACTCTTCTGAGGCTACTGCCCTAAGGGACTACGAGGCAAACTCAAGAAACTCTTAGGAATTGCACTCTCTTTGGTCAACCTCTTTGCAGCAATCAGATTTTATCTTTTCCTCAAAAGAGAGAAACCTGATCTTATTCGATACCACAGTATGATCCGTCGGAACGGTTGGCTACTGCCTCGAATCGCTAGAAAATTTCCTGCAACGAAACGGATGATGTATCATGATTTTGGCTATTTTACGCCTTATCCTCATGCACTTACCGATACGAAACAGATCAAGACTCCTCTTACTCTAAAAAATTACCTTGCGATGACGCAGACTTCAAACCCACTCAAAAAACTCTTTACCTTTGGAAAATATCTTTCCCTTTTCCTCCTCGTAAGACGATTAAAGAAAACAATCGATCTCCACCTTGTTCCTTCTGAATTTATGGAACCGATCGTCCAAAAAAGCCTCAAGATCAGTCCAAATAAAATAACTGCATTCAATCATTTCCTTCAAAAATAGTGATTTCTTCATTTCCCACTAGAAAGAAAAACCTGATCCCCTGATTTCTTGTGAGTAAAAACTTTTGCATTCCCTCAGCATCGACATTTTTCATTTGCAAAAACAAAAAAAATAACTATACTGCTCGAGACTGATTATCTCGTGATGACGCTTACTATCAAAATTCTCAACTTATTTATGGGAGCCGAAAATCGGATCCATGTGATCTTGAGGAGCTACCCACCTTTCCGTAAGGAGAGATGATACGCGATGTTACGGATAGTCGGTTTTTTTTGTTGCTTCATAATCCGTCTATCCCTAGAGAAATAACTCCTCTTCCCCGCTGAAACCGATACTTTACCTCATCAAATCAGCGTTTAATTCTGGGACTTCGGACTGAAGAAAGTGATTTCTATCCTCAAAAACTCTAAAATGAGCTGATGGCAATGCCTCTTGATACCTAAGAAAGTGGCTATACGGTACTGCGAAGTCATCCTTGCTATGATACAAGAAAATCTCTCTGACTTGTGCTTCCAGCTGCGATAACTCCCCTGTCCTGACAAATCCTACGAGTTTATCACTTTCCATATCCCCATCAAAGGGCGTTCCCAAGAGATGTAAACTCTTGATTTGCTTGGCAACTTGATGCTCTGAGAGATACTTTACCAAAAAGGCCGCACCCAAGGAATGCCCGATTAGCACAACTCCGTCTTCTACCGCTTCCAATAGTTTTTTGAAAAGAATCGCCCATTCTTCATATTTCGCGTTTTGTTTATTGGGCATTTGCGGGGTATAAACCACAAACCCTTCGCCGATCTGAGATTGTAGCTCATTTTTCCAGTCTCTCCTGCTCGCGATCCACTCTAAGTGCACGGACTTCCCCTTTAATGCTTGCAAAAATGCCGCATAACTTTCGTAAGTATCCCCGCCATGGATTAGAAGTACTTGCTGTTTCTTTTCCATTATTGCTTCTGATAAAAAGATAAAAAATCTGACGTAAATTCCTCTTTCTGCTTACACAAAATGAATTAAAAATCAAGCATAACATTCCCGCAACTCCGATTTTTATAAACTGTTGCCCAGCTTCTTCAGGGAAAAAGAAAAATCTGATCAGATTTCTGCTTTTTGTTTCCTTATCTTGAGAGAAAATTATCCTTTGAAGAGCTTTTAATGCTGATAAAGCGCTCTTTGTATAGATGCTTTTTCTAGGAGAAGACGCTAAATAGCTTGATTTCTGATTTCCCTATGGCAAAAGTTGAAAAAGAAAGAAATCTGACTATACTAATACTATCTTAAAAAGTCTTTCATCTCCAGACGTGTCGGTGTTTTTTACTTTCTATTTGGGATTCAAGATGCAAATCAAGTCAATAAAAATCTCTAATCTCTTGAGTTTTTCCTATATGAATGAAAAAGAGTTTGAGAATCAGCCTCCTATTTCTTTTTATGGAGAAGATTTAGCCTATCAAGGAGTCAAAATCTTTATTGGAAATAATGCATCTGGGAAAAGTAATTTTATCAAGATTTTGGAAGAATTTTTTACGATTTTGATTAAAGATTTTTCGTTTGATAACAGCTTTTCTGATGCAGAGGAAATCCCTACGAGAAAGGCAATTAAAGAAAGAAATATTCTCACCAAATATCTCCAAACCAACAACCAAACACCAGATCAGCCTTCAAAACTAGAAATTGCTCTAGAATTGTCAAATACTGACTATGAAAACATCTGATTTGTTTGCAAATACAGCTCTAGGATCAACAACATTATCGATAAATATAGTAAACTAGAGTTTAGATTTCCTGAATACAAATATCAGGTTATCGTCGAACAACCTCATGAACTCAAATTGGAAGCATCATTTGATGAAAAGCTTCAAAAATTCATCATCAACGAATCTAAACTGAATGAATATCAGCTTTTTGTCCTTTCTTGTATTAGATACCAAAAGCTTTTGCAAATTGTTATTACCATTTTTAATCAATATGAGAAAAAACCTGAAGAAAGAAAGCGATATTTGCTCAAGAGAACGTTTGCAATTTTAAATAACGATAGGAGCCAGCTTAATTTCAAAAATTTTATCAATCCTCATGAAATCATCAACGATCTTGAATATTCAAGAAATAGTTTGGTCGGCTATACCGGCTGTATTTACAAGATTCGAAGCATTTTAGAAAATTTTACGCAGGAAGCACTCCTCTCTTCGGATATCAGCACCCAAGAAAAAGCGATAGAAGAACGTTTGAAAAAGTCAGACTTCTTTGTTAGTTTAAACTATATGATCCAAAAATACTTTGATAAACAGTTAAGAGTGGATTATGTGAATGGAATGATTGGATTTTATATGGTGGATGAGAATGATAGAGTTTCTTATTTTGATGACCTCAGTGATGGAGAGAAATCGCTCTTAACAATGATTTTTTGACTCTATGGAAACGATCTCACGGACGGCTTTATGATTGTAAACGAACCGGAACTGCATCTTCACCCTCAATATCAGAAAGAACTCGCTGAAGTTTGCGACCAAATCAGCGAACATATTGGTGCACAGTTTATTCTCTCTACTAATTCTCCACTCTTCATCAATGAAAAAAATCTGACGAGCGTCTACAGGATGTATAAGAATAAAAATCAAAATTCTTTGGTTTCTGCGCCGAGAATCAATGTGGACTATGATGATGCGACACTGATGCATATGCTCAGATTTGAGAATCTTTCAAAGCTCTTCTTCGTTGATAAAATCATTCTTGTTGAGGGAGATACTGATGCTTACTTTTTCAGTTTTTATCTCAATTACCTAAAAACCTTCCCTGAGTGGAAGAATAAAATTCGTGATTATGAAATCATTAATATCAATGGAAAGGGGTCTTTTTCAACACGAAGAAAATTCCTCAGAAAGTTTAACATCAAGAACTATTTTATTGGAGACTGGGATAATACTGTTGATTATGGATTCTTTTCAAGGGCAGAACTCAATAGATTTTATATGCTCGCAAATAAACAGGCAAAAACTCAGAAATCTGAAAAAAGATATAGTGATTATTACAATAGACTCGTAAAAACTATCCTGACCTTCGCTCCAAAGAAGCATAAAGCGATCATAAAAGGGATTGAAAAACTTTATAACGACAAGGTTTTCATCCTCAAGGAAGGAGCAATAGAAACCTATGTTCCCCTCGAGAAAAAGGGGCTTTCTTATATGGCCTACTTCTGTAATGCCTACTTTTATGACCGAATCCTCAACCAAAATTTCAAAGAACAGAGAAAAGAATTAAATCAGATTATGAAAATTATTTTTAATGACTAAAATCAGTAAGATAACAGAGGGAAAAAGATCAGATTTTTGCTTTTTTCTTGTCTTTTGGCTATAATGATGATATATTTTACCTGACAAACCAACCACTATGGAAATTCTATTATGAACAGGAGTAATTCTCTGAATTGGAGCCCTTATCTTGGTAAGTATGTGTTCTTCTGGTCAAAAAGAAGGAAGTTTGCTCTCAAGATATTGAAAAAAACAGGGAAAATCCTTTTTTCGATGATTTTTCTTGGGCTTATTAGTTCCTATTCTTTTTTTCGTAGCATTAGGGGGATTGACCTTCCTTTTGTGTACCAAAGGATACTGCACGATTAGTACTATCGTTCCTATGCTTTCAGGATTAGGAGTGTTGTGGGTTAGTCTACGAGCTATAAGATTCATAACTCCATTTTTTATAGGAGAGGTCGCAAAAGCTGAAAATGCTAGTTACAGAACGATGAATGATATACAAAAACAAAGTATGAAAAATCTGACCAATTATATTATTTATGCGTTTTTACTTGGATTATTGCTCTTTATCTTACTTTTTATTGATCCTCGATCACTTGCCGAATCTTCAAAAGATTTCCTTGGTGAAATAGGGATTTCTCGAAGTATTCTTGAGGGAGAATACTTTGGAGATGTGATGGCGTGGCTGTTCTATGTACTTAATATTTCAGCGCTCATAGGAATTATCTCTACACTCAAAATGATTGCCAATGATTCCAGTAGAAGAGTAAATGATCGTTTGCAACAGCTCAGACTTCGTGCTTTTGAGGAAAGAAATACGAGGACTACGAGCTTTGGGCAGTAAAGTAAACAGAGTAAGGCCTTGTGTTACAAAAATGCTCGTATTTCCCATGATTTTTTAATCATTCAGAATGTTTAATTTTACAAAATAAAAAAACTGACTTTGGAATAAAGTCAGCTTTTCCTAATTAATTTTCTCTAAATACTCTTTTAACTGAATAAGAATATTCTTGTAGTCATATATCTCTGCAGCTAAAAATTTAAGTGAATATTTATTCGGTAGCTTTGAGACAAGTTTTAGGATTTCTATATGGTCATTATATCAAGAAAGTGTAATATCACTCACCAATAGATATAGCGTATCATCCAAATAAGGAGAGAAGTTGAAAATTATTTTTGCCTCTTCTTTCATTTGCTCATGTTGAGTAATGAGATATTCCCCTCGGTTTGGATGTCTAAACCCTCAGTCGGAAACAAAATTTACATGGGTTACTTCGTCCTTATCCGTCAGATTTTCTAATTTGTTTTGTATTTCATCTAAGGTTAACTCTTCCCGTCAGCAGTTATTCCCAAACATTTGGAACGGGACTGCAGCTTTTGCTAGTAAAAATTTTAGATGTTTATTTATCAATGGAGATAAATGTTTCTTTTTAGTGTTTTCAGGGGCATAAGTGATGATGATATAGAATACAAAAGATGAAGTAATTGCCAGGGCTAAACTATATCAAAATCCACTAAAAGGTATTTCTAACTTAAAGCTTGGAATGTTAAATATGATTACAAAAATTATCGCGATTACTACGACTCGATTTAATAATCTATTAGCACGATAAAACTTTAAAAGTTCTTGTCTCATAGGTATAAATACTTTCTAAAAATAAAAGTCTGATTTTTCATCGCTTTTTTACTTGTTGTGTAGATTTTCTTGCAACTATTTGTTTTGAAGAAGTGTATTCCTGACTACTATTGACGTTTTAACAAAAAAGAAATAAATCCCCTGTATTTTAGGAATTTACCCTTCAAAAGCAAAAGAAAAAAGTCATGAGAAACTATTTCTTGCTTTTCGTCTTGAGAAAAACGACAATACCAATTTGATTCTCGGGATGTTCTTCTGGAGAAACCCTATTAATCTTTGCCACTAAAATGTCGCCATAACCCGCTTCTAAAAGTTTTGAAATCTCTTTATTATCTGATCTTGGGATATAGCCAATCTTCGTCTTTTTGAAATAGACAGCTATGGCATGAGTATCGTAGGGATTTTCTGGTTCATAAACTAGCTCAAGATCTGCTCAAATTTTAAGTTGCTCAAAAATAAAAATCCCTTCTGCAAAAGAGAAGCCAGCCACATTACAGTGAAGAAAATGGAGGTATTTCATGATAATTACTATAGAGAAGTAAAAAATCATATTTTTTTGTAAATTTAGTAGCAATTTATGTTTAGCGCGGTTTACTATACGGAGCACTCAGGGAATTGCAAGAGGAAGTATTTATGATATGCGTCTAGTAACTTGTAGATATATCTTACTTCATACTATAGAAGAAAAGAGAGGGTTACCTCTCTCTTTTAAATTATTCTTTTCCACAGCATTTTTTATATTTTTTCCCGCTACCACAAGGGCACGGATCGTTTGGTCTTACTTTTCCTTCAGGTTTGACCGTTGTATGAGTTGGTCAATCCTCCACTTCAAATACCTCAACATCTGTATCATTACTGTTATCAAATATCTGAGCTCTACTATCTTGGACTTCGGTTTCAGGTTTACTTGGTTGGTGGGGGATATTTTTACTTGCTGAAGTCAGAATTTCAATTACTTTTTCTTCATTGCTTTCTTCTTGAATAAGTTGTCCTGACTGCTGAACTGCAATTGCATCAAAGTCTAATTTTAATAAATAAGATGCAGTATTATTTGTGATATTTGCGAGGAGTTCCTGGAACTTTTCAAAAGATTCTTTCTTATAAATTACTAAAGGATCAAGCTGAGCATAGGACATCAGTCAGACTTTTTCTCTAAGATTTTGCATTTCATCAATATGATCAACCCAAAGTTTATCAATAAAATGAAGATGTACTTCTCTAAAGATCTGGAATAGAAGCTTCGTCTCAAGTGTAGAAAACACTCTAGTAAAGTAAGCGTCTAACCTCTCAAGTAACATCGCTTTCAAAGCCTCATAATCAAGCTGAGACAGCTCATGGTATTCTGGTTGCGTGATATTCAAGCCATATTCCTTATTCAGAACTACAAGAAGATCAGCAACAGACTGTCCAGTAATCTCAGCATTTGCAATTTGCGTAATGATAATATTTTTAGCTTCAGTTAGGAACTCTTCTTTAAGGTTAGCAACATAAGTTTCTTGCTTCTGTTCATCGTTTTCGGCAGCAAGGATTTCGTCTCTGATACGATAGACTCTTTTTCTTTGCTTGTCAACTACGCTATCATAATCAAAGAGATGCTTTCTGATGCCGAAATATCGTCCTTCAATTTCCTTTTGAGCTCTGATAATTGATTTACTAAATTGTTTTTGGGTGAGTTCTAGGCTTTCAAGATCCGTTTTGGAAAGGAGCATACTAGCCAAAGACTGAATTTTCTCGCCTCCCATCTTTCTCATCAACGAATCATCAAGGGCAACGAAGAATACAGAAACACCAGGATCTCACTGTCTTCCAGCTCTTCCTCTTAGCTGATTATCAATTCTTCTAGATTCATGTTTTTCCGTTCCGAGGATGAAAAGTCAGAAGAAAAGGTCTTTTTCTATCGCTTCTTTGCTCGTATCTTTTTGACTAGGAATAAAGCTGACTTTTGCATAAGGAACGGTATTTGCCTTTCTCTTCCTATTAAATTGCACCGTAAAATTTCCTCATTCTAGGGCAACAGAACAAGTAAGGGTTTGCTGAGATGCTAAAGTTTTTAAATCTGCTTCATTCAATGAAACTGCCTCTTTCAAAGCATCAAGGAAAAGTCAAAATTCAAACTCAGAATACAGGTTTCCAGAAGAAACGATTCAGTTTTTTAGATTTTTTAGGAAAAATTTAAGATAATTCTGAGCCATCTTCTCGTTCAATCCCTCTTCAAGCTTGATATCCGTTCCACGACCTGCCATATTGGTTGCCACAACTACGGATTTATATTTCCCTGCATTAGAAACAATATTCGCCTCCTGCTCGTGAAACTTTGCATTCAAAACATAGTGAGTTATCGCTTCTTTATCCAAAAGCCTAGAAACATACTCAGAAGTCGCAATATCCGCGGTTCCGATAAGGATTGGCTGTCCGATTTCATGATAAAATTTGATATAATCCTTCACAAACTTCCACTTAGTATGCTGATTGAAATATACTTTATCATTCATATCTACTCTAAGAATAGGTTTATTCGTAGGGACAATAACTACTTCTAGTTCATAAATTTTATTAAACTCTTCTCCTTCAGTAGAAGCTGTTCCGGTCATTCCAGAGAGTTTCGCATATTGCTTGAAAAAATTCTGATAGGTGATCGTAGCCATTGTTTGAGATTCCCTCTGGATTTGCACCTGTTCTTTTGCTTCAATCGCCTGATGAAGTCCTTCAGAAAACCTTCTTCCTGGCATCGTCCTTCCTGTATGTTCATCTACGATTAACACTTCCCCATTGGAGATAATGTAATCCTTATCCTTGATATAGACCGCCTTTGCTTTCAAAGCATTCTCAATATGGTGGATTTCTCCATACCCCATATCCTTATAGATATTCTCTACCTTCAAAATCTCCTCAAGCTTTGCAATTCCTTTTCCAGAAAGTTGAGCCGTCTTGGTCTTTTCATCAATGTAATAATCCCCATCTTCTTCCTGCTCTTTCCCATACTCATCATTCAAAACCTCTTGAATAAAGCCTTTGGAAACTTTTTTCTTTCCTGAACAAGGAGAAAGCAGATTTACTACTTGTGCATAATAGCTGTATTTTTCAGTAGGTTCTTCTCTTGGCTGAGAGATAATAAGTGGAGTTCTCGCCTCATCAATCAAAATACTATCAATCTCATCCACAATCGCAAAATTAAGTGGTCTCCAAGTCAGACTTCTTTCTGCTAGACTTTTTACGAGATTATCTCTCAGGTAATCAAATCCTAATTCTGAATTTTCCACATAAGTAATGTCCTTAGCATACTCTCCTCTTCTGATCTGAAGCGGAGTCCCTTTGGTCACGGAACCAACGGTCAATCCAAGCCAAGTATAGACATGCGCCATCCATTCACTATCACGAGAAGCAAGGTAATCATTGACCGTTACGACATGCACTCCTTTCCCAGCAAGTGCATTAAGATACACAGGAGCAACCGCAACGAGCGTTTTTCCTTCTCCGGTCTTCATTTCTGCAATCTTTCCTTGATGGAGGATAATTCCTCAGAGCAACTGAACATCATAGGGAATCATATTCCAGGTAAGTTCATGTCCTTTAACTTTCGCTTTTGTTCCAACAAGTCTTTTACAAGCCTGTTTTACAACGGCAAACGCCTCTGGAAGTAAATCGTCCAAAGTGGTTCAGTTTTCAATTCTTTGTTTAAATTCTTCGGTTTTTGCTTGAATTTCAGCATCAGAAAGTGCATCAAATTCGGCATACCGATGATTGATATCTCTCACAAGTGGCATCAAAGCATCAATCTGCTTTTGATTGTAATCACCACCAATTATATTCATAATCCAATCTAACATTTGCTTCTTTTCTAGTAAAAAAAGATAAAAATCTGACGTTTAATCAAGAAGAACTTATACGAATTTGTATCCATAAAGCAAATTGTTTTTTGCATGAAATGCTCCTCTATTAAGAAAAAATGAATAAAACGTCTTGCATCTTCTTTAGAAATAATTAAAAGAATGGAGATTTATCTTCTAAAAATAGTAAACTATGCAAAAAAAATGAAAAACAGCTGGTGAAAGAATTGATGAAGCAACAGGAAAGATCCAGGAAGCAGCTTCAAAGTTCGGGGAAAAGATTGGGAATGGAGCCGAAGAGACAAAAAAGGTTGCAAACAACGTTAAAAATTGGCGAGAAAAAGCCTCTGTTGAAGAAATTGTGATGACGTTGATTGGAATCGTTTTGTTGATTTTGGCGCTGATTCAGCTAAGAGAGTTCCTTTGGGGAATTATTCTTCTTTTGGCAGGTCTACTTTGTGTTATGGGATATTTTAATCCTTTTTTGAGAAGTTTGATTGATAGATTTTTTTCAGAATCAGAGGACACTTCTAAAAAAGACACCCATTCAAAGAAAGAAGACTAAAGATAAGAAGCAATTGATAAGCCCTTATCTTAAATGATAAATTTATGGGGAAGGTTCAAAGAAACCTGATTTCTTCCCCTTTTTGGTAGCCAAAAGTTAGAAAGGATTATTCACATTTATCTGTTCTTTACCTCCAACATGACACGTGAAGATAGAAGAAATTTTATCAAAAATATTTTTAGACTTGCAATTGGTGTTGTACTTCTTGTTGCTTGTTTTTGATATTTAAAAAATCATCCGGCTGAACAGATAGCACTTTATTCTGGATTAAAAACTATTATTCAGAAAGGAGAAGTGTTGGCATATAATGTTTTATGAAGAGATGGTAACCAGCTGGCTAGAAAATATGATTTGGAAAATAGGTATCTTGAGCTTATCCATCGTGCCGAGGAAAAGGGATGTAAAGATACGGAGTTAGTGGAGGCTTTACATCAGACGTATGAGACTTTTTTACAAGAAGATAAAAAGAAGATCAGTTATTATATCGCAAAATACATGATTCTTTTTTCAGAATATGACTCATCTGTTGAAGAATGTTCATAGTTTAATGCCTTTGTTGTTTCCTTCTTTAACTTAAACTTAACAAGCAATTCTTCCCTTGCTTGTTTTTTCTATTTTTCCGAGTCTTAGTAAAAGTGGCTCAATATCTTGTTCAAGTGTTTTTTCGCTAGTTCAAAGCTGGAGAGCTAGAGTTTTCACTCAGACGGGGCGATCAAAGTGAGAAAGAATTTCCAAGTATTTTTTGTGGAGGGGCATCATTCCACCATCGTCAATCTGAAGATGGTCGAGAAAATCGCATCGCTCTTTTTTGTCCAGATTTTGTATCTTTTTACTAACAACAAAATCTCTCATTTTAATTACAAGATTGTGAATCTCTCTAGGAACAGGTGAGACTTTGCGTGCAAAAGTGGGTAATAAATCTGCATTGTATGGGATTTGATATTTTGCCAAATATTGCTGGATGATCAAGATTTTCTCCTCAGAGGTATATTCCATATAGTGAAAATGGTAGACAAATCTATTTTTCATTGGTTGTGAGAGAGATTCTGGTTTTGTGGTGGCACCAATAAGTGTAAATGGACTAATGGGAAGCCTAAGATTTCATCCCTCTGGCATTACCATATCTATTACAAAATCTTCCATTGCAATATATAAAACCTCCTCTATTGCTGGTCTTAACCTGTGAATTTCATCAATAAAGAGGATATCTCAGCTTTCTAAACTATTTAAAACACTGACTAATTCACTGGGCTTAGAAATAGCATATCATGTAATAATTTTCATTCTCGTTCCCAGCTGATTGGCAACGATTCATGCCATCGTTGTTTTTCAAAATCCACTCGGTCAAGAAAATAATAGGTGTCATAATGGACTTTGCCTCTTTTTTGCACTATCAATTGCTGTTGCGAGGACTGATTTTATTGGCTCTTGACCGATAAACTGAACAAAAGAATCTGGTCTTTGCGGATTGGTTGTGGATTCTTTCGTCTCTAGTCAGATTTTTTTTATTTCCATATTATTTTTGACAAATAAATCATTAAGCTTACTATGCTGATTTTAGGGAAAATTTCAAGAGAGATTTTTTTAATTGGCAAGTAATTTTTTTTGTAAAGCAAGAAAATCTGACTCCTCCATTTTTGTCATAATCTTCTCCCTGAAAAGGAGCATAATCTTTGAATTTTCTTCCGGGTTTTTGAGATATGAAAGCCGAAGTCCTAAAAGCATTTCTTCGTTTGTCCCCACGCATTCAAAAGGTTTTATGCCTGATATCCCCATGAGTTCAGAAAAAAGTTCTACTAACTCCTCTGCATCAAAAAGATCTTTCCCAAAAATTTGTATTACCTCTTCTTTGCCTAAAAATGCCCTCATCATTGTATAAATAAAAGCACACTTTGGGCATTCTCAGCACCATAATTCATCTCTTGCTAGTTTTGATCAGCTTTGGTGAAAGTTTCTATTACAGCTGGAGAAATTGTAAAAATACTTTGGATACTTGCAAAATTCTTGAATTATTCTGATTTCATACCGTTTTCTTAATAAGCTATAACTGGAGAAATCTTCGGAAATTCGCTGGTGAACATAGTTTTGAAAATCCTGTTCAAATTCGGATGATTTGCTCCGTTGATGATTGATTTCTAGTCCATGATAAAAAGTATTACCTTCATCTGCACTTTTCTCTAGTGAGGTAATGGTCGCATCATAACCGTATAAATAAGACACAACTAGAGATACAAAAGATATTATGCCTGTAATTGGTAGATGACCGTTGTAATATCACTGATTAAGGAGCTTTTTAATTTGATCTAGATCGAGAGTCCTTTTAATAATTAGCCTTTTTTTTTCAGTTGGGATTTGTGCAATCTGATGAAGCGGAAAATCGTTTCAAAAAGAAAAAAGATCGAAAGTCTGACCTTGACTTTTGAGCAGTTCTACCGTTACTAGAGAGTCCTTTCCCCCTCCGAATAAGACCAGCGATTTATGTGACTGCATGAAGATTTTTTTTGAATAGTTTGGTGCATCTTTGACAGAAGCAAATTCGGCTAATCATTCAGGATTTATCTGGTTTCTATAAAAAAATTCTCCGAGTCCTTTAATATAAAAATTATTCCAAAATCTTTTTTGCTCTTGCGTTAAATCGAGCGTATGGACAAGAATCTCTTTTGTTGGAAAGAGTTTGTAGTAACTGACCCCTAATGCGAGATGGAGATGAGCAAGAAGTTGTTTGAGTTCGTTTTCATCATTTTTTACTATCGGGAATTCAGATTTCTCTTTTAAAGGAGAAAAATTGATCACTTCTGTAAAGGATTCGCTCTCATCAAATGAGTAATGGAATTTTGCCTCATAGGTATTTGGATTCCACTCAAAACGGGAAATATTGAAAGTAGAAAATGGTTTCATAATTATTCAATTTCAAATATAAAGCCACTGTGATCTGAGCCTGGAATTTGGATTTTTTTCAGATTTTTAAACTTTAGGTTTGGACTGAGAAATACATGATCAATATGTGACTGAAAAAAAGGGAAATATCCTCAAGCTTTCCTTATCCATAACGGCAAAAAACTAAAATCCTTATGGACATTCAACATTTCTCATAAGTTTCGAGTGAAAAGGATTGGGAATGTTTTTGTGATGTTTTCTAGTGTGCCACTAAAGGATCCTGCGAAATCTCCATAATAAGCAGATCGTGGGCTTGTGTTAAAGTCGCCAAGCATTATAATTTTTGCATTGCTTTCTCTTTGAGATTGATGATGAGTAAAAAAGTCTGATTTAATCTGTTTTAATTGCTGATTTCTCTTTTCAAAAAATGCTTTGCTAACCGGTGATGCGGTATGAATTTCATAAAAATAATATTTTCATTTTTCTGTATCGATAGAGAAATAGCCATATCTCCAACTTGCCTGAGGGAAGTCGTCAGCAAGATTATCTATTGGATATTTACTAAATACGATGCTTCCAACCGCGATTTTGGATCGAGAGGTGGTATTGCTATAGGGATAATGTGCCGCAAAAAAATCCTTCAAAGCTTGACTATGCTCTTCACTAAACTCGACAAAAAGAACAACATCTGGGTTTTCATCTAAAATCATTTTTTTGATTGCATCTATATTGTTATTTCCCATATAGATATTGGAAAATAAGACTTTAATTGGACTATCTATTGATTGATTGAGTTCGTGTAAAGGCTGATAGAACTGCTTAATCGGTCGCAAAAAACAGCCAAAGAGGATTCAAAAACAAAGGACAAATAGAGGAGATAGACGTCTTTTCAGACTGTTTTTTTGTTTGTCTTTGTAAAGAAATTTCCAAAGCTGAAAAACTCGGAATAGCAGTCCTAAAAAGCTTATTATCGATAAATGTGGCAAGAAACTGATTGCCAATTCTCCGAAATAACTCCCTTTACAGAGGATCACAACTCCTCCAAAAAGTATTCGCAAGGAAGCAAATATAAGACTCATATTGCTCTATTTACTAGGGATAAACGGTTTTTGACTATATATATTCTTTATGAGAAAGCAAATGTTTTTGTTTTTATCAAAAAGTTGGGGAATAAAACTAAGGAAAATCTTGCTTTTAGCTAAGAAAGCACTATACTTTTTCCTAGGAGTCTTTTATCTCCCTAAATAAAACGATGTGAGGAGTAAAAAAAGCAATAAAATTCTGATTTTTGGGACTTTTCGGTTTTGTTTTTAGCGTGAATATCCTTTTTGCTAGCTCTGGTAGAAACTCTGTTTTCTATCAGAATTTAAATCAATTATTAGTTCCAGTAGAGGCTATTCTTCGCCAAAAAACACTTTCTCGTTATCAGCTTACGAGGCTCTTGAATGCAGTAGAATGCTATGATTGTATAGTCCCGGATAAGAAAACTCATGACAAATATAGTGGAGCCTTTTGGACGGGGTTTGTTTCGTTACCGGGTAAAGATTTTAGGGATATTGAGTATCTTTCCTGAAAATATCAAGGAGTAAACTATTATTACTGTGTGGCAAAGGTTGGTAACGATGATACCATGAGAGGATACCCAGTTGCAACCAGTAATATTTGTGCTGGAAAGTTTTGTGGATCAAGAGATATCAGTAAGGCAGAATTTTTTCAAACCCTTCTCAATCTTCTCACTGATAGGATAAAAGGCAACTACTCTGCCCCATGGAAAGACATTAAAGAATGGGTTAATAAAAGAGACAAAAAAAGCTATGAATATAAAGTATTTACTACTGCAGAGATTGACCTAATAAATAGAAAAGAAAAACAGACTGAAAAAATACAGTCGAGACTTGAATTTTCTACATATCTTAAATACTGTATGTTTCATCCGAACGAATGTGGATTTCAGACTTTCCCTACTTTACCAAAAGGATATTGGCCTCTTTCAGAGATGAATATTCTGATTCAGGCGGGAATTATTACTCCTCAGGATGTAGAAGGCTTTTCTAAACCTATTAGTACGGAAGATGCATTAAAAAAGCTGGATCTCCTTTATGAACTTCATATTCAATGTGCATTAAATGTTGACTATGACTGTGATGGACTAGTAAATCATGAAGATAATTGTCCTTATGCTTATAATCCTGGACAAAATGATATGGATAGCAATGGTATTGGCGATGTTTGTAGTGATGATATTGATGGTGATGGAAAAAAGAACCCGCTTGGTCTGGTTGATGATACCTGAAATATCAATTATTCAGTACTTATGAAAGAAAAATCTGAAGATCCGACTCCATTAGGAGAAGATACAAGAGAAGACCTTTATTTCATCAAGGTAAGCAAACTTGATAATGATCTGCCTTCTTTGGTAAAATTTGAGATTACGAGTCGTGAGGCTCCATTAGCAGTTGAATGGGATTTTTGAGATTTTACAAAAGGGAAAGGTATTAAAACTCAGCATATTTATACTGCTCCTGGTTTAATGACTGTTATTGCAAAGGTTACTACCAAAGGAGGGAAAGTCTTTGTCCTTAGTCAGCAACTTTCGCTCGGACAACCGCAGAGCACGATGTATTGACTTTCTATCAAGTTGGAAAATCTGGATTCAGCTAATAAAAAAGCAGTCTTTAAACCTGAATTTATGGGTAAGTTCGACTATTTCCAGCGAGAAAACACGGCAAATTGACACAAGGAAAATACGGTTGCTGATGCGTCTTTTACAACTCCTTTGGAAAATAATCAGAGAAATAATATTACCTTAAAAGGCTATGTTCAAGGTAAACTTGTCGCAGTAGCTTCAATAGATATTCTTGATCAAAACTGAACTTTCTTTGGATTTAGTCCTGAGTATTCGCCGCATCTCAAGACTACTACGACCAAAATAATGACGACACTCAGACTTCAGAATCTTAGTCTTGGTGCTATAGATTCTGTTAAATGGGATTTTGGCGATGGGACGAATTTTATTGATAGGAAACTCGTAGCTACACATACCTATACCGATCCGTGAAATAAAGTTCTGATCCAGAAAATTCATTTAAAAGATGGCAAAGAACTCATTTCAACGTCATTTATGACGTTAAGATCTCTAGAAGAAATCTGAAATCAGGCAATCAACATCATTCCAACCTATAATGACAAAGGAGAACTCGCGCTTTCCCTTCAGGTACATTGAGCTGTCTCATCGGATCAAATCACATCCCTAACAACCTTGGTTAATAATAAGAATAGTTTTTTCACTCAGAAACCTCAATTAAATACTCCTTTCTTTTCCGTAAAGAATAGACAAGGACAGGTTAAAGTTACGAATAAGTTTCGTATTGGTTCAAAACTTCAATTAGAAAACCGTGGAATCGCTGTTTTTGATCTTCAAAGTAATGCTCAGAATGCTTTTTCTCTTGATCCTCAATCTGCTTTTTCTTGACTGAAATGTGATTTTGATCATGATGGGATTCCTGATCTCTATGATGATGATATTGATGGTGATGGTATTAAAAATCTTCTTGGTATGGTTTATTATGAAAGACCTGACTGTAAACTAATCGTTGGAGATAACATTGATCAACCTCGTTATAGTGCTCACTTTTGAATTTGTAGCTTGGATAACTGTCCGATAGAAGTGAATACAGATCAGGATGATCTTAATGCCAATGGAATTGGGGATATTTGTGAGCAGAAAGATCCTGACTGCTGAAATGGGGTGATTGATAAGGGGGAAACCTGTAAAACTTGTCCTCAAGACGTAGGTCCATGTACTGCTTTTTGTGATAATGGTAAGATTGAGGCGGCCGAAACCTGTAAAAACTGTGAAAAAGATGTAAAAATCTGTCTCGTAAGCTGTGGGAATGGGAAAATTGAGGAAGGTGAAATTTGTGATGATGGAGATCAGAATTTTCATAATGGTAAATGTTCTGTGGACTGTAAACCTATGGACCCTAAGAAACCCCTCTGCGGAAACGGAGAAATCGACCTATGAGAAGACTGTAAAAACTGTCCTGAAGATCTCAAGGATATTTGTATTGATGATGGGAATAAAAAGCCTAAATGCTGAAATGGAGAGGTCGATCCGTGAGAAAACTGTTCTAATTGTTATCAAGATGTCCCTAACTGTGATGAAGATGAAGATTGATGTCCCGATCCAGAAGATCCATGCCCAAAGCTTCCTGGTAATAATGGAAGTTGTTGTCCAAAGATTCCTGAGCCATGTGGAGATTGAGAATGCCCCCTTGTAACGCCTCTTTGTAACCAGTGCCCTTGTCAATATGCGGACTATAGTAATACCCTACAAAATAATGATTCAGTGAGAGCAAGGCTCTGGGATCAAGGCTTTAAAGTCCATTATGATTATTCAAAATTTGCTGCTATCAAGAACTTTCTCCAGAGAGAGTAAACTTATACTTTTAAGCGAATATCGCGTAATTGTTGATGATATCACAACTTTTTGAAATGCTGATTGATTGCCTCAATCAGTTTTTCTTTTTCTTTAAAAATTGCTATTTTCATAGCTTGATCTGAAGTTTTGAGGAAAAGTGTTTGGTATTTCAAATAGCCTTCAATGAGCTCGTCTGTGACGATATTCTTATTTGTATTTTTGATATGAAAATGGTCCTTAACAACCTTAAGTGCAAGAGTGCCTAGCATACGATTCCCCTGAAATTTCTCAGAAAAAAGCTTACGAGAGAGGTCTTGAAATGCTTTCATCATCAAAAAAGAAATAAATCTGATTCAAGATAAAGATTTGATTCTTGTTGTCAAACAGAAACTCGTAACTTTTTATTCTTTTAGAAACTAAAAATACTCTATACTCCTCTACTACTAATTTATCTCGAATGCTACACTCTCTTTCGAGGATATCCGCTTCGTTTTTTCCCGCTGATTTAGTAAGAAAAAGCGCTTTAGAAACAAATTTCGACTTATCTCATGCTCAAAAAAATAAAACTCATTTTGTCAATATCAGTAAATATATTTTTTTAAAATGGTTATATCATTTAATCTTTTTAATCTAAAAAGTTGAGCAAAGAATAGTAGGAAAATATAATTGAAAGGGAATTTTATATTAAAAAAATATTCATCATGGAACTCAATTTGCTAGAGTATGATATTGACTCTATAAAAAGCATTTTACAAGGCATTCTCGATAAACACCTTCCAGAAGGACAGCAAATTTCGGTTCAAGAGCTTGATGAACAGGGAATCGTATGGCTTATACAACTTCTTTTGGAGAAAGATGATAAAAAGAGAGAACAGCTCTGGCAAGAGATTGAAGATAAATATCAGGTTGCTTCTAATGATTTTAGAAATACCTATCAAAAAATCCTTGAAATTTGTGATCAAATTAAAATCACCGAACAACTTGGAACTTCACTTGTAGAACTCAAATCAAATATCAAAAATGATCATTCACTTTCACAACTAGAACAAGAAATCTGACTTTAATCTTTTAAAATTTACTTCAAATGACCGAATATAAAAATCAATCAGTAGACCAAATCCAGAAGCAGCTTTCAAATAAGTTTGAAAAAGACAAGCAGTGGCGAATTAGAGATGATCAAGAAATCTATCTTGCTGGACAAGCAATTATTAATGAAACCATAGGCAAGATCCAGGAGAGTGGGAATAACTGGGGGAAATGGGCTTTAGAACCTTTGAAAACTTTGGAAAAGGAACTTGTAAAAAAGCATAACGAATGTCAATGAATAAAGCCTTTTACAGACAACAAAAGATTACTCAATGATATTGCTAATCAAATTGATAATGCAGAGAATATCGTTCTTGAGAGGCTTATGCGTAATAATGCCCATAGAGATATTATCCAAAAAAATACTGAAACAAAAGATACTCAAGGTAAAGCATTTTTTAACTTCGACAAAAAAACAGACACCTATATTTTTACCGATAGAAGTAACAAACCGAAGATTCACGAAGTTTTAGGTCAGTTTTTTACTAATGATAAAAATATCTATACTATTGATTACTCAAAATGTACTAATTTGAAAATCAAAAATAAGATGCAACAAGTATTGGGAGGTACAAGTAGTTGCCTGATCAAATATGATAAAAAAACATGAACATACAACCTTATTAGCAATGGAGCGATCGTAGATGAAAGGGCTTTAATTTGGGAATGAGTTAGCCTCAGAACTGCTGCTTCTACTGCTGTCTCTGCGAAAGCAACAGAAAGGGCAAATAACGAAAAAGCTACTGATTACAGCAATCAGAATTTTATCAATTCTCCAAAATATCAAGAGTGTTTAAAAAACCTACCTGCAAGCCTGAGAAATAAAGTCTCCTGAGAACAGCTCGCCACACTCATTAGCAAAACAGAATCAAGACTTGATACAATCATAAAAGAACAAAAGAAACTCTGATGGGAACTTGAACAAGATCAACCTATTTCTAGAATTCATTTTGGATCATGACTTATGGAGGGGCATTTTATCAATCGTCAAGGGACAAAAAAGGATATAAAAATTTGGGAAGATAAAGATTCATTTAAAAATAATGCTCTTTATGATATCTTGGACAACAATGAATGACCATACAAAGACTATCTTACAGCAAGGATCAAAGCAAAATGGGCACAAGCAGATCATCTCACAAAAAGAGAACAAATCTTTAGTGGCTCTCCTGAAAATAAAGAAAATCCAAATTTAGCTCAAGCAGTGGAAAAATTTAGCTATGGACTAGATATGCTTTCTCAATTGGTAAATAAAATCGAAGAAGAGGACTGAACAAGTAAAGAAGTTGCAGCGCTGAAAGTAGCAATCAGAAACTATAAATACAGTCTTGAAAATACCGTTCCATCATCTCAGGAACTTGATGAAGCTAAGAAGAAATTGGGAGATCTTTTTGTTGCGTATAAAAGTAATGATTGATCTTTTTCTACGATGACAGATAGAACTGCCAGAGAAACTGTTGCAAACCTTCTTAGTGGAGATAAGACGAACGCACAGAGAAGTATCAGAGAATTAGGGAAACATCTCAATGTATGGTGAAATACAAAAACAACCTTCCTTAGAGATGAAATTGCGAATAATGAAAATATCAGTTTTGGGAAACTTGATTATACAGAAGCATTCCAAAAAATTGGAACTCATCTTGAAATCGGAGAAAAAGATACAGAAGCACAAGTCCAAGAGAAAACAAAACTGATTGATAATCTCTATAATTGTGCGAATGCTCAAGCTATATTTTCCCTTTTGCAAAATAATGGGTTGATACCAGCAAATGCACCATTTGATGAAGAAATGAAAAAAGGATGCCAAAAAATCTATGACAGCTTAAGAGCTAAGAAACAAGCTATTGAACAACTAGATTATAGTCCTACTGCATTCCAAAGCAAAATGGAAAATGAAAAGATCAAACTAGAAGCCAAAGGGAATAATCTGACTGATGATGATAGGATCAGACTTCAAAGTATTTCAGTAATGCTTGAGAATCCTCAAATGCTCCAGGAAGCACTCCAAAATGCAAAATCAGCAGCTGAAAATAGTCTCAAATATGAAGGAATTGGCGCTCTTCTCAAGTGAAGCCTCGCACTTCCTTTCGCAAAAAATGGAGGAAAAATGAGCTGAACCAATGCAGATATCTTCAATAGGAGCCATGGACTCAACGGACGATTAAGCGGAGACGAAACAGCTGTCTGGTTAAATGAAATTGGGCAGATGATCTTGGAAGAGGTTGCAATCTGTATCGTTGCTATCGCACTTGGAGCAGTGACAGGAGGCGTCGGGACTGCAGCTATTTATGGTGCTAGATTCGCTCTTAAGGCAGCACAGGGTGCAAGATATGCAAATAAAGCCTATAAGATCAAAAAAACATTTACGCTGATGAGAAAACTCTCTAAAGCAAGGAAATATTGGAAAATGTGAAGAGAAGCAGAAAAACTAGTAGCTGCGAGCCGTCAGGTAAAAAACCTCCAAAAAGGCGCAAAATTAGCAAGAACACTAGATAAATCATGAAAAGTTGCCCAAAGGATGAAATATGCAAATAAACTCAATGATGCAAGAAGATCTCTAGATGCAATCAATAAAAGCAATAAATTTGTGCACCTTAATGACCTTGCAAAAGTTTGAAAACTGACTGATCATCTTGCAAAATGAAGTCATCTTCTCTTCGAAGGAACCTGATTTCATTTGAGTAGTACTGTTCTTAGAAATGCACTCCATGGGAAAAACCTTACAGATGGGGTCAATCCTTTTGGCTATACTGAAGGACCTAATGGTGAACAAATTTCTAATTTTAGGGGATATGTCCAAAGTATCGCATTTTTAGGGATTTTGAAAGCTATCGGACAACCGCTCCAAAATCTTACTGGAGCTGCATTACAGAAGCTTATGGGAGAAAAAATTGCAGCGAACTGGCTCGGAAAAGTTTTACAATGAATAACATCGGTTGGAGCTGAAGTTGTAACGCTTACATTAGCAGAACAAGGGATCAGCCTTGCACTTGATCAAGAGCTTTCTCCGATTACCGTGGAAAGTGCAATTCAGTCCTTGGGAATAGTCATTGGACTGAGGCTTTATTGATGGGGATCAATAAAAATCAAGAATTGGAAAGAATGAAAGCATTGACTTCAAGAAATAACGGTAGAAAATGGAGAACAAGAAGTTGTAATTAATGGGAATGGGACTGTAATAAGCTCCAACACTCCTAACATGCCAGTAGGTAAAAATGTTCTCATTCCTCACATGAACGAATCAACTTGATCCTACGGAAAAAATGCAACTCAAGAACAGCTAATGAGAAAAAGAGAACAACTCAATCAAGAAATAACTAAGCTAAAAAATCAAGGAATAGCTTCCACAGACTCTAAAATCACCACACGTGAAAATGCAGTAAAATTCATAGAAAATAAACTCAATTCAGCAAGTTATAAGGAAATTTCCTTGCCAGAATCTAATAAAGTTTCTATCAAAAATGCATCTTATAATATGGCTGTTAATAGGTATGCTAATTGACTTGCCAATGTAAATGAATGAATCCCAAACGAAATTCAGCTTCGTCCTGATGGAGCAAAAATAGCTCTAGAAAGAAATGGAAAATACAAAGATATTTTTGATCAATATGAAAGTTTACTAAAAGACAAGCCACAATGATATGAACAAAAGTTACAAGATCTTGATTTTATGTTGGCAGAGAGAGTAACGCTTGATACTTGACATACTGTTGAATATACAGCAAAAGCTGAAGCTGAGACGTCATTTAAGGGTCTAAAAAAAACTTATCCTGAATCAGAATTACGTCAGTTTGAAAGCTCTGATGGAACTTTTTGCATTAGAAAAAAATCACCAGAAGAAATAAAATTAGAAGCAGAAATACAAACAAAAAAAACTATGCGTGATGCAAATAATACTATGATTCAAGAATTATCTAGAAAGATTGATAGCATAGAGGCTGAACTCAAAGCCAAAAAAGAAAAATCAGTAGAAGAAAAAGCAGATGAGATTTCAAAGAACAGACCAGAATATTATGAAAAATATGCAAAAGAAAGTCATACGGACCTATACGAACAATATAAAACCTATCAAAATACCCCAAATGAACTCAAACGTTTGGATACTGAATTTGCTGAAAGATTAATTTTGGACAATCCTTGAGAAAGAAAAATACTTTTTAGTGAAAAGTGAACGCATTGAAGCATCAAAGAAAGAGCAGAAAACTCTTTTAAACTGCTAAACAAAGTAGAAAAATATAAGTGAAATATCAAACTTGTACAAAAGACTCTCAATTGAGAAAATGTTTTTGTCATTGAGTTGGTCCCAGAAATAGGGAAATTAACTAATTTAAAAAATCAAGTCATTGCACAAGAAGGAATCATAAGATGAAAAGAATCTGAATTATCTAAAATGCAGAAAAAATGAGATGGACTTGAAACCATTGATGAGTCAACAACTATGTTTAGATCTCAAGAATCAACGGGAGTGCACAACCAATGAAATGACATACAACAACAAAAAACAAAAAAACTGCTTAATATCCCGAGAGAGGTCATAGAATCTAATCTTTTTAAAGATTTTAATCCTACCAACAGCAAGGAAGTCTATGAACGAATTGATTCTCATCAAGAAACTCTTCAAAAACAAATACAAAAATTAGGTATTTATGATAACAAGACAATTTTTGATTACCTTGAGAAATCGCCCGATTTATCTTGATATGAAAAAACCACCCTCAGATATCTTCAAAAAAGATCTAAGAATATAAAGTTAAATATTGAGACAAAAAAATGATCTCTACGTTCTTTTGCTGACAAAGAAAATAAGGTAATCAACATCAACCTACAGCAGATAATTGGTGCATTCGGGATATCAAGACTTAAGAAAGGGAATATAAGTTTTGATCATATGTTATGAAATTGCTTAATTCATGAATACAATCATTTATTCTTTAATCCATGAAAATGATTGAGAAATAAAACGATTCCTGCACGAGAGCAACAAGCGTATCATGAACTACAAACTATACATAAAGAATACCTCAGTCATGCAAAAAATCATCCATATAATGATATTATTCGTAACACATCTGGAGAAGAAAATCATGTAAATAAACGAGAAGAATTTTGTACAGAAGCTGTAACTGAACCCCTCTTTATGCAATATCTTTCAGAGATCCCATACAAAACGCAAAAGAGTAGATACGAGTTTCTTATGAAAGAAAATCTAAAAGAAACATGAATAGAAATCAAAGAATGAACAATGCTTTATAAGATGCTTGACCTTTACTACACCAATATCAACAATACAAGAAGAGAATATTTTAGTTGGAATAAAAATAGAACTCTAGAAGCTAGAGAACTTCAAGAAAAACAACAATCTCAATGAGTAGAGTTCCATAAAAATAATGCAAAATGATTTCAGGAAATCTTGGAAAAATATATAAAAAATAATGAAAAAATGCTAGTATGAGAATCAAAAAATATAGAATTTTCTAAAAGTGAAATAATGAATACCTTGATTGAAAAGGGGTTTCCTCCAAAACATAAGGTTAAAATTGATTGACTAGAGTTTTTTTTAAGTGATTGCTATCAGGGATTGGGTCGTCCTTGGATAGTGGGATATACTACTACAGGAGAAGTTAGATTATTTTATAGATCTATATCAGAAGGGATATGGAGAAGTACTGATGGATCAGACGGACCTATTATTAGTAAAGCCCAATATTTACCTAATGGTTCATATGAGACAACCACTCAAGTTGATTTTCGCTTAGGAAAAATTTTCGATAAGATTCCTATGCAAACAATGCCATGAATGCAGAGTCCCTGGTATTGGCTTTTAACATTCGGACGTAAATATGGATTTAAAAGAGAGATTAAATATTTTTTAAGACCATCATTAGAGCCTGAGATTGAAATCCATTGAGGTAAACGATCTGCTGTAAATTATTATAAATGAAGAGATATGGCTGAAGTTAAAAAAGGGTATGAAAATTATAATATAAAACTACAATTAGAAAAAATTATAGATAGCTACACATTTTCTCATAAGATTCTAGGAGATATCCATGTGGATATTGTGAGAGGGAACATACAAGGGCAAGATGTAAATATTCATTTTGGTCATGCAGTTAATAATGATCCGAATAGTGTCTTTATTATTAGGTGAGATTTTGCTGATTCTAAATTAAACACATTTTGAGTACCATCTAAACAGCTTAATCTAGGACCATTAGTCGCGAAACCTTTGGATTATAGAGTGCAGGCGCCGAGAGGACGACAAGATCTAAAAGACTATGGCGATTATGTTGATATTCGCCCTCTTTATCAGAATACTCCTCTTATAAAACAATATAAATCTCAATTTTTGAGTAAATCAAGCTCATAATACCTTTCACTCTTAAACTTGAGGAATAGGACTATCCAAAAGCGATATTTTTTGAAAAAAAGTTTTTAAACCTAAGATAACCTGTAAGAATCTGCTCTATCTAGGAGATAATCAGCTTTTTTGTCTCCAGCTAGAAAACCCTTCTATTGAAAACACTTCCTTATAAAAAAAACAAAGAAATCTGGCTGCAAACCTCCCTTCCCCAAATAAAAAATGGAGTGTCACAAAGATCTTGCAACACTCCAAATCTCACCTTTTCAATCACATCTCAAACGAAGAAGTTTTCTGTTCCCAATCCTTGGGGACATATTTATCCCGTTTAAGCGTCAGAATTGTATTTTTTCCCGGCAAGGAATAAGGAACCCAATTAACTCCATACATACCCATAGCAGGAGCCACCTCTTCTGTCGGGACTACCCCACTTGGAGCACCACAATATCCCTGATTTTTCCCATAATGATTGCTCACCGCACAAATAGCAGATAAAAAATCTGTCAAATTTGCAGAAAGCACTCCGCCATGAAAGTACTCTTTTTTACCATTGGGATATACTTTCACAACGACATCAACCTTAAGCTCAAAAGTGCCCGTTTCCACATTAACTTCTCCAGAACTTGACTCTACCCAAAGATAATAGCCAAATTTCCTAAAGAAATCAGCCTTCATTTCTTCTAAAGAATAGTCGGAATCGACAAGAATCTCTAGATTAGAAACTCTTGGCTCTGGAAGCACAGCTTCAGGCAAAAGAAAGTAATCAGCAAACAGCTGCTCATTAAAACTTCCTGCCAAAGCATGCCCATTATTCTCAAGCTTCAAACGAAATGCAGAATTGCGATCAAGAAGATAGCCTTCTACCTTCCCTTTGTCAATCAGACAGACATTTCGAGCTTTGGTTCCTTCCATATCATATTGATAAGCAGCAAGCATACGCTCATCTCTTGGACAGTCCCAAATCTGAATATGCTTCAACACCTCCATAAAGCCTTCTTTTGCAACCCGTTTCCCAAGTTTCCCTTTGAAAACCGTAGAAATTCCATCAGCAATATAGGCTCCAGAGAGCATATGTCCCGCAATTGCTTCATGAAAAAGCGTGCCAACAGCCGAAGGTGCAAGAAGGATAGGGTAAATCCCGCTCTCTAACTGCTTACGATCAAGCGTAAACAATTCCTCTTGAAGCCTTGGATGCAATTCGTTGCTAAGATAGTTCCTGAGAGCATCACAATCCTTAAACATTCCTGAGAGCTGAGATTGATAAACCCTCCGCTGTCCATTCATAAAATAAGGAGTGAGCCCAAATTGAACCCTATCACGATGGTCGATTACACGAGTGCCCCAAGAATCTACAACGATCCAAAGCTCTCTCTTTGCAGTCAAATTCGCATCCACAAAAGCAACATGTTTTGCAAGATAACAATCACGGACTACATGTAACATTTGGTCACAAAGAGAGCTCGGAATTGGCTCTAAAGCACTAACCTCAAACACAGAATCCTCTAAATAAACCACATTCCCATGCGCTTCTTGATGAAATTTATTGTCCTTTATTTTTGAGGCATAAAAATAATCGATCAACTCATCTATTGCGAGAGTAATCTTTGTCCATCCCAAAGAAAGAAGACCTTCGGGCTGAGCAAGAATACGCTCTTGCATTGGAATCGATACACTTGCTTCCTTCTTCTCATTAGCAAAGAAGAGAGTAAGCAAAAGCTCGGGAGCCGACTCATTCTTTTTCATTGGAGTCATAATACCATTAGCATAGGTAATTTCAAAAGACTCCTTTTTCAGATAGGCAATCCTGATACCAGAAATCGTATGGCTTGTAATATCGCCATTAGGATTCTCTTTCTTGATTTTCAGCGATACTGCCCTCTGCACCAACTGTTCTATAGCTTGAACAATTGGTAAATAATTTACACTTTCTTTTTTTTCCATTTTCTTATTTTTTAATTGTTTAGCATATTTTTATATTTTCAACTAATATATAAGGTAAATCTATCAAAAGTCAACTAAAAAATAATTAAAGATAACTCTATCAAAACCAAATAATAAAAACAGCTTTAAAAAGCTGAATCTTAATGAAACTTATCAAGCACTTATACAAGAATTTTCACAAGAAGAATCGCAGATCCCATAATAAAGACTGAAAGAAATTGAAGAACTATAAGAAGCAATAAATTTAAAATATTAAGATACTCCCCCGCCTTTAAAGTGCTAATCTTTTTTCTTAGATACTCCCGAATAGGAAGACCTACAAGAAAAAGCCAGATAAGGCAAAAAGGAAGCATCGCTCGAATACTTAGTCCTCTTAAACCGTACTGATAGGCAATAAGCATACATAATGCACAGATAAGCCAAGTTAAAATAAGATAAAGAAGCCTTGTTATAGGAGATAATGCTCAGATTTTTTCATGTCGAATTTTTTCCTTTTTCAAAAAAGAAGAGGGATTTCAAAAATATATCACTGCGATAAAAGCAAATATACACAAAACTGTATAAGCAAGATATTCAAAATCCATACCTTTCCTCACAGATAAAGTAAAATACTGCTATCTAAAACACACAATTCTTTTCATCTACAATCACCTCAACCAAACTCCCCTTTTCATAGTTTCAAGACAAGGAAATCTGAAGATAGTTCTCAGTCCATCATTTCGCTTTCCCATTTTTCACTTCTTCAATCAGGACTTGGAGTTTTTTCCCATAGTTTTGAGCGACAAAGGCATCACGAACCTGATCGCCGATCTCAATCAGCTTTCTATTTCTGCTTTTTCTGATTTCATTTGGCACTTGATCTGGGAGCGAAGAAGCAGGGATCCTCTCGGCTTTGTGATGATCAGAAAAAGGGAAGGCATGGAGCTTGGTAATACGATACTTCTGCGCCGCCTCGCAAGTGATCTCAAAATCCGCCTCCGTTTCTCAGGGAAAGCCAGAAATAAGATCTGCACCGATGGAAACAAGTTCCGCATCAGGGCGATTCAGATTTCTTAACTTTTTTAGGACTTCATCAAGCTTTTTGCTATCATAGGCTCTATTCATCAGCTTCAGCACCGCATCAGAAAAACTCTGAATGGAGAGATGGAAATGCGGACAGAACCTCTGATTATTCATCAGCTCAAAAAAACGATCATCAAGATACTGAGGGTCAAGACTAGAAATCCTGATCCTTGGGATCGTGGTTTGCTCCAAGATCGCTTCCAAAAGCTCGTTGAATCTGCTTTCCTTAGCATTCCTCGTATTGCTCGCACCCCAAGCTGCAAGATTGATCCCGGTCAGCACGATCTCTTTCCCACCTTCCGCTTCAATCTGTCTAATCTCCTCAATAATCTCCGGCAAAGGGCGATTTCTATGCGCTCCCCTCTTGAAAATCGTGAGACAGAAACTGCAATGATTATCGCAACCGTTCTGAATGATCACATAGTTCTTGGTTCGGAGCTTCTGATTCGGCATTTTGAAACTAAAGGCGAGTTCGCTCTCCTCCTCTAGCGGATCTTCTGCAAGAAGCGTGATTTGCTCTTGATAAGGTGCTAATTCAGGATAATAGGCAAAAAAAGCTGATCTTTCCGTGGCTTTCCCTTTGGTGATAGAGCCACAACCAGCGATAAGGATTTGCTTGCCAAGTTTGGCTTGTTCAATGATTTCTTTGACCCATTTCATCTTGGCTCTATCGGTCACCACGCAGGTCGCAACGAGAAAAAGATTATCATTCACTTGATTGGTTGCTTCAAGGTGAGCGATTCGTTTATTGAGGTAGAATTTATTGACTTTACAACCGTAGACTTTGTGCTGCATGATTTTTTTAAATATTAATTTTAAAAACTAATTGGATTGAAAAAAATTATATTTTGGATCATATCCAAAAAAATCTTGCAAATTAAAATTTGTTACATCAATATTCAAGCCCTCAAAATACTTAGAAATAAAATAAGAAAATATTTTAAATAAGGGATGATCAAACCTTCCCTCAATTTTTAATCTTAAAATAAAAATCATAATATCTGTAAGTTGCATGGCATCGAAACAATCTGACGAAAGGAAAGAAAATCTTACTGTAATATCATCCCTATTTGTAACAGCATTAATTTTTCTTCTTATTGCATCAATATATGTATTTTCTAAATCATTATATCTGATGATATTTCCATTCTCTTGAGTAGCTGTATGAGACCTTCTCCTATCAAATAAAACGGTTATGGTTCAAACATCTTTAAATATTGTTTGAATCCCGACTCATGTGCTTTTCATTTTTGAGGCTATTCAAAACGCAGCTTGTCATGAAAAATATCTTTCATATCCTCCATTATTGGCATTATTAGTGACAAACTGTAAGTTCTTTTTATACAAAAAACTATAAAAAGAAACAGACTTCTCTTGTATAAATAAATCTAACCACCTTTTAATTAACTCTATTTTATTTTCATCATTTTGAGAAATTTCTGAACCATGGATAGTGTTCCAACACATAGGAGACTCTTTCCTTGCTTGTTTAAGTTTATTCAAAAAATCAAATTCTCTAGAATCGGAATCTGTAATTAAATATCCCATTAAATTGTATTCCTCTCAATTTAGAAAAATTTCTGTAGACTCATCTATCCAAACTGTTATAGCCTTTTTCATCCACTATCATTTAGGAGGATACGGATCTTTTCAATAACTGTTTCTTTCTCTAATTTTCCCTTCCCTATTATGGATAAAGAGCTCTGATCTATTTTGTTGTGCTCTTTCTCTTGCATAAGATGTAGCCTCTTTTTGAGTTCAAAAAACTCTTGATGCTCTTTCTGAATGGGGAGTTTTTACCGCCCATCCATTAGAATGAGGGACAACATGTTGATTTTGTTTATTCATACAAGAATAATTCAGAATATAAAATAAAATGCAATAATATTGTATACTAAAATTTATTGTATAAGCAATAGCTTTTTTGCTTTTAGGCTGCGTTGGAGAGTTTCTTTGTAGAAAAACTCTGGAGATCGGTGTTTTGTGTCATCATCATATGGTAAGAATATAAAAGGGCTTACTTTTGCTATTCTCAGAAAGGTTTTAACATCAAGAGGCGAGTTTTTTGGGGATGAGTAGCTTTTTTGACCGTTTTTGTGATAAAATTTTGAATGTTTGGACTTTTTTGATGAAAAATATGAAATCTTTGTCTTATTTTTCGCTTTTTTGACCCAAAAAATGAAAATCTGATCCGTTTTTTAATTTTTTTCAGCATTTTGGTCAAATCTTTTTGTATTCTAGCATTTTTTTGACCGTTTAACCCCATTCTTTATCGGTTTTGAGAGTTTTTTCGGCGTTTTGATGATTGCGTTGGCTATTCTCTCGGTTTTTTGGGTTTTTAGTATTTGCTTAATGGGGGTATAAGGAACGCTGAGGGAATTGCAAGGGGAAGTTAAGGGAGATTATTCTATTTTATTCTTTTTCTCAGCATCATACTGATTTCCTGTCAACATAGATTTTACATAGTCATCATCCATCAATTTAAGAAAAACACCAGCTTGACTAGGATTGCCAACTACAAACTTTCCATCATCTTGTATACGAATTTTTATCTTATTATTTGTATCTCTTACAAACAAAACAATTTGCTCTTTATTTACATTAAAAACAGGAGAATCTTCTTTTATTTTTAATAATTTATTCCTTAATTTTTGATTAGTTTGAATAATTGTTTCTAATGTATTATTATCAGATAATAGTCATTGCCTTGTAAACATTGATATTTTTTCACAAGCTCTTTGATTCATAGTTGTTTCATAGCCAAAATCTTTTTCTAATTTTTTAAAATCCAGAATAAGAATTTTATTGTTAAAATATAGAAATATAGGCTTATAATTAAAACGTATTGCATCTGTCGTTTGGAATTGATCATCCATTTTTCTGAGTATAAACCAAGTATCACGTTGAAGAGCAAATATTGGAAAATAAGGAATATAAAAATTTATAAAACGATCCGTAGTTCATAATCTAATAATAATTCCATCTATTCTAGCTGCTTCTTCTCGATTAAAATTATCGTTATTCCCAATTAAATCATTTGTTAAAAAACTGACTTCTTGAGTATAAGTATCCTCAAAAAAATATAATACATTATTTCTTGTATAATCATAATTATTTACATTTAATAATTCAGTATCTTCTCAAAAGGTTGATAATTTACCCCTAATCGCTTGCAAAATTTGTTCTTTTAGCTGTCATTGAGCCTCATTTGTTATATTAACAAGTTTATCTTGATATGAAACAGGTCAATTTTCAGATTCATTTTTCAGCCTAAAATAAAGCATTGCATTATATGCTTGATTTTCGGAGGCATCTTCTAAAAATCATCTTACTTGTTCTAACATGATATTGTTATTTAAGAATTAAAAAAACTTCATCATCTACTAAATAAATTCCTTTAGTATCAGATAGTTCATTAAAAGATTTTCTATCTTTTCTTTTTAAAAGAACACATTCCTCCTTACCGTTTCCTATTTGAATTTCTGCATCATAAAATCTCCATCACAATAATGGGATTATTGGATTTGATGAAAACAAATTCGTCTTACAATAGAAGAATCAAATCACAACAAGAATAAAGAATAAAATAAGTAAGGAGCGTCGATTTATTGTTCAATCTGTACTCTCAAATGAAACAAAAACAAAAGGAATGATATAAGTCATAAAATAACTTAAATATACATGATTATTAGTTTTAACCTTTAATATTTTAATAGGACGCTGTGTCATTTGTAATCTTTTATAAATTATATGCCAAGTAACACCAAATATAATTAAATATACAAAAAAACATATCAATAAAATTAGTGTACGAAGAGAAGATGTTCGGAAATATTGAAAACAAAACAAAAGATATAATGGAAATAATGATATGATATATGACAATATTTTCATGATTATATAACTATTAACTATTTAAATCATAGATCCCTAATAATAAAGTGATTATATCAAAAATCAATTCTAAATCAATCCCTGCTTCCTCGCAATTGTCCTCGCAGACTCAGTCAAATACTTCTTTCTAATTCTAATCGCCTTTGGCGTGATCTCAACCAGTTCATCAAGTCCGATATATCCCATCGCCTGCTCAAGAGACAAATGCACGATTGGATCAATTCTCATCGCTTCATCATTTCCAGAGTTGCGGACATTGTTCATTTGCTTATTGATAGTAAGGTTTACGGTCATATCTCCTCCCTTCAGATGCTCCCCTACGATCATTCCTTCATAGATTGGCTGAGCTGGGTCTACGAAGATTGGTCCTCTCTCCTGGAGTTTGAAGATTCCATACATCATCGCCTGTCCGGTGGCAGACGAAATCATAGATCAAACTTGCCTTTTTACAATTTCTCATTTAAATGGTTCATAGTGAGAAAAGGCTGAATACATGATTCATTCACCCTTCGTCGCGAGGATGAATTCTCCACGGAATCCGAGAAGACCTCTGGTTGGGATGATAAATTCCATCGTTGTAAGTCCGTTTTCTGAGCTCATATTTTGCATGAGTCCTTTACGAGGTGCGAGCATATCAATTACTGTTCCAGAAAGCCCGTCTTCTACGCTAACGACAAGGTTTTCCATTGGCTCAAGTTTTTGTCCGTTTTCTTCTTTGTAAATCACCTGAGGTGCTCAAACTTGCAGCTCCCATCCTTCTCTCCTGATCGCTTCAATCAATACTCCAAGATGAAGCTCTCCTCTTCCTGAGACGATGAATTTTCCTTCCACCATTTCTACTTTCAAACCAACATTGGTTTCAGCTTCTTTTTCTAGTCTTTCTGCCATGTTTCTGGTCGTCATATATTTCCCATCTTTCCCAGCAAAAGGGCTATCATTCACCAAAAACTCCATACTCAAGGTCGGCTCATCAATCGCAATTGTCGGTAATGCCTTAAACTCCCCTACTCCGATCGTCTCTCCCACGAAAATATTTGGGATTCCACTGATAGTCACGATATCACCACACTCTGCCTGAGGAATCTCAATCCTCTGGAGCCCGAGCGTTCTAAAAATATGCGCGATCTTCCCAGTTCTCTTTTCTCCATCATTTCCGATGATCGTTACCTGGCTTCCTGCGTTCAAAGTTCCTTGATACACACGCCCAACTCCAAGTCTTCCCAAATAATCATCATATCCGAGGTTCGCAACCTGCATACAGAAAGGGAGATCAGATTTTTCAGCTGCTGGAGCTACATTTTCCAAGATCGCATCAAAAAGCGGAGTCAAATCCTTCGCTTCATCAGTCAGATTTTTCATCGCATAGCCATTTTTTGCACTCGCATAAATAATCGGGAAATCTGCCTGCTCATCGCTCGCTCCAAGCAAAATAAACAGATCAAAAAGCTGATCAATCACTCGGTCAGGCCTTGCCGCAGGTTTGTCAATTTTATTAAGTACAACCATCGGTTTGAGTCAGAGTTCAAGACTCTTTTTAAGCACAAACTTGGTCTGCGGCATCGGCCCTTCATACGCATCCACTACGAGGATTACAGAATCCACCATTCTCAATACTCTCTCTACCTCGCTTCCAAAGTCCGCATGTCCTGGGGTATCTACGATATTGATTCTCTCGCCTTGATATTCAATCGCGGTATTTTTGGCATAAATGGTGATCCCCCTCTCCCTTTCCTGATCGTTATTGTCCATAACGAGGTCTTGCTCTTCCATTTTTTTGAGCGTTCAAGACTGCCTCAAGAGCTGGTCTACGAGTGAAGTCTTTCCATGATCTACATGGGCGATAATTGCGATATTTCTAATTGCCATTTGCTTTTTGGTATAAAAAATAAAAAACCAGAATCAGATTTCTTGGTTTTTTCTCTAAAATCAAGCATTCTGAAATTGGTTTGAGTATAGCGAATTGAGGAGTTTTTTCAATGAGAAAAGAAATCTGATTGTGAAGGGCACACTTCTTTCTTTTAAAAAGTTTAATCTAAAGTAAATAAAACTTTGTATATAACTCTTTTAGAAAATCTATTAGCCCGTTTTATTATTTTGAAAAAATAGCTTTCTGTCTTTGATCAGCAATATAATGAGGAACAAAAAGAGAAGTCAGGTTTGAATAATTTTACTATCTGGATGCGTAGAAAACAGTTCATTCCCGACATTTGCAAAGACATGGAAAAACACCACGATCCAGAGATTTCTCCCGGATTTATAATAGAGCCAGTTAATCAGAAAGACAAAAATGACTGCAGAGCAAGCGAAATTGAGTCAGTAGATTCGTCCTTCTGTCACGAGATTGCTATGATAATAGCCCTTTATAGTTGCAAGTGGGAGATGCCAAGCAACCCAATAGACTGCAAAAATTAGATTTGTCGTGATGAGGCTAAATTTTCTCCTTAGCGTATCTATTCCATAGCTATGTCGAGCCAATTCCTCTACAATCGCAGCAAAAACAATAATAAACCAAGTAGAAAAAAGGAATGTAGAAAAGGATGGATTTCCGGTAACAACAAACTGCTCTACGCTATATCCTAAAGGAAGAGAAATGAGTTGAGCAACTAGCAGTGATCAAAAAGTAAGTCCTAAGATTACCAAAAGATATTTCCAAAAACTTTTATCAAAACGTGTGACTCTTGCTTTGAGATCTTGCAAAAGGATAGGGTCTTTTTTGATCAATCGAAATGCTATCAAAGCCGGCATACTGACTCCAATAATTGCTAAGCTATTTTCTAAGATAAGAACCGATCTTGTTTGCTCTTGGTGACTTAGATAGGCACAAGCAAAGAAAAAAACCGCAGAGAGCAAAAATGAAGCGATATAAAAGGTTCGAGGTTTATTGTAGGATTGAAGCGTTTGCATTTTAGGGGGAGGAAAAGAAATAAAAGTGAGAAGATTTTCTCAAAACAAACATGCAGGACTATAAGTATTCAAAAAGTAAATGCAATAGCTGCATTCCATAACCTACTTTTCAGGGCATAGCTACATTCCTTCTTGCTTTTTTGAACGGAATCTTTACCTTTAGGTGAGTTTTTTTATTCTTTTGAAGCTAATGAAGACAAAAATCTGACTTTTTCTCTGATGAGTGTTCTTTTTGGGTTGTGGATTATTGCTTTGGTTTTGAAGTACTCCTATAAAAGCTCAACTTCAAGAATCCTTTGATACTTCTACAGGAAGCAACACCTACAAAAAAATTGAAACCTGATTTGATCTTGAGGCTGATGCGTCTTATCAGAAATTCCTTGATGAAAAGCATCCTTTTCAAGATCCTCAGTATGAACCAAGTGATTTAGTACCGATTCCTTCAGATTTTACCTTTAATTCAGCGAGGAAATTTCAACTTAGACAGGAAGCCTGAATACAATTTGCCGATATGGCTTGGCACTTCCAGGATCACTTCAAAGGCAAGAAAAAACTCAGCATTACCAGTGCTTATAGGTCATTTAAACATCAACAACAGCTCTTGAAGTCTTATTGCAAGAATAAGCATCAATGTGCAGAACCTGGAAGCAGTGAACATCAGGCATGACTTGCACTTGACCTCTGAACGAACAAATGAAAGCTCGACAAAGTAAGCTTGGAACGATTGCAAGAAAATGCTTATAAGCGAGGTTTTCATCAGACCTATCAAAAAGGAGCGGATATTGATGGAAAGATGATAGAACCATGGCATTGGAGGTATCTCGGAGTAGAACTTGCAACTGAACTGCATCAAAAGAAGCTGAGTTTTGGAGAGCGATTTTATTCTCAATCTGGGGAATCGCTCCCCTCCAAATTATAATGAGAAAGATTTAATGTCTTGAAAATCTTCGCCAAAATCTCTATACTGCAATCAGATTTTATCCTTTTGAAAAAATAATGCCACAATCAACAAACCCTAATGATGAAAAGCAACTTACACCTGAAATAGATACAGAATCAGCCGATCTTGAGGCAAAATTAGAAGAGGTGAAAAAAGAAACACAAGATGCTCAAGCTGATTTTACACCACTTTTGAATAAAATCAAAGAGCTTGAAGCCCAGCTTCAAGAAAAAGATGAAATTGCAAGGAATAGTCAGATTGCTTATTTAAATTTGAAAGCGGATTTTGATATTCTTCAAAGACAAACTCAGCAAAAAATTGAAACTGCGGATCGTGATGCTATTCTGAAGGTTGTAAAGGATATGCTTCCTTTTATTGAGAACCTCAGAAAATCCCTCCTCAACCTCTCAGATGAGGCAAAATCTTCTCCTATGGGACAGGGGCTTCAAATGATGTATGAGAATCTTTTAAGAAGTTTAGAAAAGTTAAAAGTCAGACCAATTGAAGCGACAGGATTGGCTCCTGACGCGCAGTTTCATGAACCTGTAAGTATGCAACCAACGGAAGACGAGCATCTTAAAGGGAAAATTATTCAAGAGTTTGAGCAGGGGTTTATTTATGAAAATGGAGATGACAAACTTGTCATCACTCCATCCAAAGTTATTGTTGGGAATTAGTGTAAGCTTCGCTTTTTATTGATCGGAAAGAATTACTGTTCCTTTATGCTGGGATAGTAATTTTTTTTGTTCTTCTTTTAGGTGGTGTAGTCCCTCTAAATAAAGCTCTCATCTATGTTTACTGAGTGCAATAATTTGCTGATTGCTCAGCTCTGAAATTCCATCTAATCGAAGGTTACCTGAATACTTGGAAAGTATTTCTGCTTGTTTGTCAGTTAGTTTTTTGATTCCTCCTAAGAAAAGGTCTTCTTGTCATTTTGATTCTGCTTTTTTCACTAGTTCTTGAGCAAACTCATCAGTCAAAAAGGTAATTTTATCTGTTCCTTGTGTTAATTCATTATCAAACTGCTCTCTTTGCATTTTTTTCAAATTTTCTTGTGCTTTTTGGAGAGAACGATATCCGCTAATCGTTTCTCTAGAGAGAAGTCCTTTTGTAATAGGGATATATCGCTCTCAGTGTTTGATAATTCAAGGAATATTCGGACTACTTTGAATACTTTTATCCATTTTGATTGATACCTCCTGTAGTTCCTTAGATTTACCTTCAGAAGAATCTACTCTTGCCATAGGGATTTTGAACTGTGAGACTGGATTTATGATAGGATTAGGGAAGAGCTCTGTAAAGAATCCTTTTCATTGCTCTGTCTTTTTTGTCAGTTTTGTTGATTCTGATAAAAGTTGTTTCTGGTCTTGAGCAATACTTTCTGCCAATTTAAAAGGAATCTTTGTCGCAGGTCAGGCTTTTTTTTGAGGAAATGTTGGCCTTAAATTATCGCTCCATGAAGAATTTTTATCGCTTTTAAACTGAGTAACTCACTCATTTGAGTCAGAATTCTTTTGATTTAATTTTTCAAGAATTTTATTGATCGTAAGTGGTCATGGAATTCCATCAGGAGTCAAGCCAGATTCCGATTGAAACTTTCCAATATTCTCCTTGGTCATATTTCATAAGATACCATCTACTTTCCCACTTTGATATCCAAGCTTTCTGAGTGCAATTTGGACTGCCATCGTCGCAGGAAATTGATGCTCTTTTGCAAGCTCATTCCAGGACTTATCTTTTAGAAAAGAAAGATAATCTTTTATGGCATTCATATTATATCTTACCTCTTTGTCATCTTTTTCGAAGAAAAATTCCTTTTTCAACTCTTGCAATTCAGATTTTACATTGTCAACTACCCATGCTATTTCCTGAATTTCTGATGCAGATTGACTAAATACTTCTTTCATTGTTTTTTTGGTTAAATAAAAGAGAAAATTCTGACGACTCTCCTCTATTTTAACTAATTCTTCACGTTTTGTCAATTTTTTGCACTTCTTGATTCTCTGGAAGGAATATCTATTTTTTAATTAATGATTTTTTTTAGCTCGTCTCGAGTCAACTCCTCTAACCTTTTCTTTTGAAGTTCCTCTGGGATATAATAAGTCGATATTCCTTTTTTTTCTCACATTTTAGCAATTCTGCCTAAAGTTTTTCTGCTATAAGGAGAGAAAAAATTTAGAAAGGTTTCCAAGGCATTCATATCTATGTTTTCAATTTGAGCTTTTTTTGTGCATCTAATAAGACAACTTTTTACTTTTGGTGCGGGAGAAAAGCATTTTGCGGGGACCATTTTTACATAGTCTACTTGATAAGCTCTGTTAATAAGCCACCAGAGATAAGACTTTTTGCTCGCGTCAGATTTTATTTTTTCTCAAACTTCATTTTGAATCATAAAAAATCATCCTAATAGCTTTGGATCCTCTTTTCGAAATAAAGATTTAAAGATTGGAGAGGTAATATAATAAGGAAGATTTCCTACGACAAGAGTATGTTTTGCGTCTCACCCGTTCTTCGTTAAGAAGCCTTCAATGTCAATATTAAGAATGTTCTCAAAATAGATTTGTTCCCTGTCTAGTCCTATTTTATTTAGATACTCCTCCATTTTAGTATCTTTTTCAATAACAAAAAAATCTCTTGGAATCTGATAGATTTTCCTTGTTATTGCTCATTTTCATGGTCAAATTTCCACCAAAGTTTTTGCTCAAGATTCTTGATAAAGTTTCTGAATTGAATCACGAATAAAATTAATTGTAGAGCCATCTTTGAGAAAATTCTGTCCTAGATAAGTAGACATAGTTAGACATTAGATAAAATTCAATATCGAAGAGCATTTTCTTTTGAAACATTCGCTCAGATAAGTTGTTTTACTTTTATTCGAACCTCTGCTGACTGCTCTTTGTTGGCATCTATTGCTATTTTGATGATAGCGGAAAGAATAGCATCCAAGATTCACTCTTCATCCATCCTTTTTAGGAGATTCATCTTACTTGCCAACAAACCTGGATGTTCTATTATCTTGAGAAGCTGAGAAATTTCTGTGAGAATCTCAGGTTTTTCAGCCTGTTTTTCTAATATTGTTTGTAATATTCAAGGTCTTCCTAGTGCAATTTTTGCAAGGAGCTGCGTCTTTTCTGGAGATTCAGATTCTCCCAAAACTTGAATTAAATACTGCTGCATTTCGGTATCTGAAAGAGGAGCAAACCTTATAACCATTGCTCTTGACAGAATTGTTGGAAGGATTCAGCTTTCATGCTCTGCTGTAGCAAAGAGATATCTATTAGGGAGTGGCTCTTCACAAGTTTTTAGGAAAGCATTCATTGCAGCATTCGTCATTCTTTGAATATTTTCCACAAGAAGCACCTTTTTACCACTAAAAGAAGACTGCTGTAACCGATTTGTCAACTCCCTCACTCCCCTATTTTCGTAGGTAGTTTTTTCATCAATAGAGATTATTTTGGCTTCTTTAGGAGTTTCAACTTGGATTGAGTGAAGTTTTCCTAGCTCTTTCGAAAAATCCCTTAGTCGAAGAAAATCAGTTTTGAACTGTTCTCATAGAAGCTCCTCGACAAAAGAACGTAAAAATGTAGTTTTTCAGATTCCTCCCGGTCCAATTAAAATCAAAAAGACAGGCTTTTGTTGCGACTGATTATGAGAAATGTAGCTTCTAAATTGTTGCTTTGCTTTTTCGTTTCAAATAGCTTGCATTTAAGATTATTTTTGGACAAAAACAGTTTGGAGTATAAGTTTTATATTTTAAAAATCAAATGATTTCAAAAAAGATAAGATTTTACAAGTCATTATTCTAATAAATGTCAATCGATAAATCTTAAAACAAAAAAATAGCAACAAAAACTGACTGCTCTCTCAGCTTTTTCCTTTATTTTTATGTTGATTAACGTATACTTTTGAAGTGAGTTTTTTATTTCCTCGATCTTTTCTATGAGGGCTAAAAGAATAGTTGGTCTTATCATTTTGCTTGGATATTTAGCGTTTAATGTTCTTTCTTATCTAGGATATCTTGATTGGAATAGGGCATCTGCATCTTCCGATAATATGAATACCAATACTCAATTGGTTGCACTTTTGGTTGATGAGAAAATCTATCCAAGTATTGAAGCTGATCTTAAACGATATTCAACTCAGTATATTCAAAAACATTATCCACAAAGCAAGGCTCTGGTCCTGAAAATTAATACGAGCGAATATGATGCTCCAGAAATTGCAAAACTACTTGAAAATCTCTATTTTGACTGACTTAAGGATCAGAGTTCTCGTCTAATTGGATTAGTTTTGGTGGGAACTATTCCATTTCCTGCAGTTAGGTATGATAATTATGTTTTCCCCTCAATCTACCCTTATGTTGATTTTTTGGATCAGAAATATGTTTGGAATCAAGAGCTTGGTTATTTTACTGAAAACAGAAAAGATGGGCAGCCCGAAGTTTGGCATGGAGTAATTGATTTTGATCAGGATATTGATGCCTATCACAAATTTTTTGATAAACTTAAACTCTATGATGCAAAGCCTAAAAAGTTTGTCGCGAAAAAAATATGGTATGATGATTTTATTGCTCATAAGAAAAATTTTCTTGAAGAAACCTATCAACTCTATCAGAATAAGCTTCTTTTTGCAGAAGATTTTATCTATCATCGTTATACTGATCTTTTTAACCGCATTTTGCAAGGGGATCAGTATAAAGAACAGGCTTTACAGCTTTCTTGATTGGCTGAGGATATTGAGTCTCTTGTTCAAGATTTTGCCGAACATGGTTCTGATACGACCGATCTTCAAGACTCTATTGCTAATATGAATCTTTGAGAGATTAACGATTCAATTAAAGAGCTTGAAAAAAATACTCAGAACATTACTCCAACAAAACTTTTGGAAAGAAAAATTGGTTCTTTTCTAAAAGATTATCACGAAATTATTGGTACACCGTTAATGCATACTATTTCTGAGAATGTTAAAGCAGGAGATAGATGGACTGGCAATGATAGTCATTATCAAAAACTCCAGCTCAAAGATCAGCTCCTTCTTGGGAACACTAAACTTAATGGGATTATTAGAAGCATTAATGATAAGCTTGAGTCCTTTGTGGATGAAAAAGTAGAAAAGGAAAAATATGCTATGAAAAGTACTATTCCTTATTATTATAAAGAAAGTCAGATTCAGAAAAAAGCAGCTTTTTCTAAGGATTTTATGTATTCTTATCAAGATTATACTATAAATAACATCTATAGATTTTTTTATTTTGGGAAAGACGCACAGACAATTACTAAAGCTGTAGATTATTCTACCTATAGAGGAACTTATAGGAATTTAAGCGGCCTCGTAAACTATCCTTCCATCATCCAAGATCAACAAAATCCAGCAAAGTCGGATTGAGATGCAACTGACCTCACAAAGAAAGGACTTTGATCTTCTTATGATGTATATTCTACTCAAGTAGATGCTAATAGAGGATTTAATATGCTTAATACAGAATGAGAATACGAGCTGTATCAACAGGAGAAAAGACCAGCAAAATATATAATTAAATGTGTAAAATGGCTCCCCTGAGGATCCTGTTTAAGATGGAGTAGAGAACCTAAAAAGAAGTGTCAGGCTAGCGGAAAAAATTGTGAGACTTTTACACATTTTTGAGATAGAATATGGTGAGGTGCATCTCCAATTAATGTTGACATGGATAAATATCAAAATCAGATTTATGCCTTTAGTGGAGAATATGACTTTAGACGTGCTACAAATGAAATTTTTGATATTGGAGGCTCTGTTCCATTAACAGGAGAAGAAAAAGAGGCGCCTCATTTTCTCGCGGAGAAAATCTATGTCTCTCCTATTCTAATTGCAGATAAGGATGGCTTTCATTGGGATAAAACTATGTATGATACCTGACTGATAGAAAAGACGTGGTTTTTTAATGTTCCGCTTGAAGGGAAGCGTTTCATTCCTCTTGGTACTACAGGCTTTTCTCTTATAAAAAAACAGAAGGGGAAGAAAGATATAGGTCTAACCTACAAATATAGGATTGTCCCTTCTGAGGTAAAACATGTTTCGACTACTACAAAAGAAATTCATGGATATACTGGACTTAGATATTCAGAGGATGCAAAAGAGCGAGGGTACTATATGTCGATTAAAGACGACCTTACCTTTACAGATGAGACAGGCTCTCTTGCATTAATGAGTTGATTGAATAAACTAAAAGATCACCTTTCTTCGTTACAAAACTCATTTAAGAATGCAGCTAGCTCTCAGTCCCCAGGAACAATTGAGAATCTTCTTTCTGAAGAGATTTTGAAATCTACTACAGTACTCTCGAGTTGACAAGCAGAACTTTGGTCCCTTTTCAATGATCTAATTATCAGGAATTATGCTAGTGATGTCCAAGCCACAATGCAGAGTATTGTTTTTAGATCAATTGTTCGAACTAGGAAAATTGATATTTTCTCTGAATGGCATGCAGACATCCTCAAGGATTTTGATAAGTTGATTAAGTTTTTACTTGAAAAAAAAGCTATTGCTTCACAAATTTTTAAGCTTTATCGTGAGGTGATTAATCAACTTGATGGTATTGATGCAATTATTAGCTCAGGAATTGCCAATAATAGCAAAGAAGAACAACAAGCTGTGAGAGAAGCATGGACAAAACTTCGTTCTCCATTTGCTAAATTATCTGCCATTGTTCATGAGATCAATACTGATACAGAAGATTCATGTATTTGTAATCATGGAATAGTTTGAACATGTTTATGAAAAAAAAGGAAGGAAATTGTAGGTAAAAGCTTAGGTTCTTCATGTGGAAACTCAGGAGAATATGGATGAGATGAAAAAGAGGGATCTTTAGATATAATGATTGAAGCATTAAAAGAGAGTAAAGAGGATTTTAAAGAAGACTTCAATTCAGATCCTGAAAATCCAGAGATCCTTTACACAGGCATGAACATCCTCACACCAAGTAGGCCTATTGATTCTCCAAGATATCTTTCCTTTCAATGAGTAGGAGGGAATGTTGTAAAACTTATTTATCCTGATATCATGAAGGTTGAAGTTCTTGATCAGGCTTCAAAAGCTATTTCTAGTCAATCATGAGCACTACTAGAATTGAAAAGTCCGGAAAGTATTCGCAAAGCCCTTAGTCAATATTTAAGCAATAAAGTAAAGGAATACAATGCTATCATCAATGCTGAAAAAGCAAAGGCAAAACCAATGAATAAGCATTATCAGTATATTTCTGTTGTTGATAAATTAGGAACCCCTCTTCTTGACTGATGAGTTCGCCCTTATGAAAATTTTACTTATGAAGAGTTTTTGGAGGCGATTTGAGGTCAAAAAGTTCTCAACAGTCTTGCAGAACTCCTCTACTTTCAGAATGTTTCAAATCAGGTAAAAGTTTACTACGATGAAATTGAGAAAGATCTTGCCGGTAGTAGAAGTGATTTTGATATTAATGAAAGGATTTCGTATATTATGGATAATTATCTCTCACAGAAAAGAGATGCTTTTTATGAAGAGAAAAATGCTGATCCAAAATTTGTCATTCCTAGTTATGCCTCAAAAGGTTATGAGGTGTGATTTATCAATTCAGATAATGATGATGGACTAACCCCTTATCAGGATCAGATTTTTGATGAATTTAATCAACGTATAGAGCCAGTTCCAGTGCTCCTTCCAAAGGACAAGGAAAAAGCAGAACAAGCAGTTGAAGTTCAAAATATCCAAAAAGAATGTTGATTTGATATTAATGAAGGCTTGGATATCTATAGTTTTAAGACAAAGAAATTTGAGTGGCGAGAAGGCTTGAGATGCCGATTAGACCAGGTTAAGAAAAAGCCTTTTGAGATAAAATTATCATTTGAGGGTTCACTTTGAGCTGTACCGATCAAGGAATCTATTAGTGGCGATATCATCGCTCCTCTTGCTGATTCTCGAGAAAATTATAAAGGAGAGCTCAAAAATACCTTTTCTTTTCACGATATTGAGAATCCAGAAACCGTGAATAAAGATAAACAGGTGCAAGAAATTCTGAATCAAACCCAAATTACGCTACAGAATAAGAAAATTAATGTATCAAATAGGACTTGAAGTATAAATATTCTCTCTTTTAAGGATTATTGAAATCTCACTGTAAGGATTAGCTCAACAGGAGATAATTGTCTTATTATTAATGGACAAAATACCTGTGTAGCGAAAGCCTCTCTTTCAAAAAATCCTTTTAAACAAGGCTTTGATATTCCTTTTCAACTTGGAGACACAAAAACAGGGAAAATTGTTGCTACTTTAGAGTTCTGCTGAAAAAGTGGGAAGTGTGGGATTAGCCCTGTTACTGTGTTTGCAGTCCCTGGGCCCGTTCATAGCTTTGAAATAGGTCTTCCTAACGATAGAAAAATACTTGCTGGAGCTTATAGCCTTATCTCTCTTAAAGCTTTTGATCAATATAAAAATGAAATTCAGAGAACTCTAGAACCATATACTATTGAGGTTGATAGAGGTGGGCTTATTGTTTGATGACAGGAGAGGCAAGTTCAAGAGGTCAATGATTTTCAGAATTTAACACTGTTGTATAGAAGTCAACCTCAACTTGGATGAACTGTAAAATTTTCAGTAAAGAACCAAAGCTGAACGCTTCTTAAGAGTGATACTGCGACCTTAGTCCCTTGAACACTCCATGTTGAGCAAAATGGTCGCCGCTTATCTTCACTCACCTTTCCGCTTACTCCTGGAAGTTATTTTCTTAGCTGAACAAACGGAATGGAAGTAAATGAGAAAAAAGCAATCCCCCTTGATCTTGTTCTAAGGGATAATAATGGTAGAGAAATTCCTATTACTACCAATGTTATCTTGGGTGCGCAAGAAGAATTGCTTCAATTTATGGTGCTAAAAAAATCTACTGATGAAAATGGCAAGCAATGGCTGAATTTTTCTAATACAAATTCTCTCTTTGTAGAAAAAGGAAGAGCAAAAGTATATCTAACCTCAAAAAATATTGCTGGGGATGAAGAAATTTCTTCGCTTATTCCGTGACTTGAGGAGCAGAAAATTAAGGTAAATATAGAACCAGGACCTCTGGCAAAAATGACATTTTCACTTAATTCTGACAAAATAGATCCTAATTCTACAACCAAAGGAAAGCTTTTCCTTATGGATGAACGATGAAACAAGCTCAAAAAAACAACCACCGTCTCTTTGGATAAATTGGGACCCGTAAAGGTTTCTCCTGGTGCTGGAAATATAGCTATTAACAACGGAGAGCTAAATCTCACTTTTACCTCTGACCATAATTATTGACTGACTAAATTTTTAGCAGAGGCAACAGTTAATGATAAGAAAGTTCAGACGACGACAAGTATTATTACTAGAAAATTTTTCCTCGAAAATGCGATAAATTCAGGATTAAATATTATGTATCTCAATCTTTTCTGAAATGATCGGTGAAACCAGCGATGATATGCATCTTGACATAGAAAATTTTCAGAAGATATTATAAAAAAGTCTGAAAAAACTTTAGCGATTACAACTCAGTTGGTAGATCTTAATAAAATCAAAAAAGCGAGTGTTATCTTAGGTAAAAACTCTTTGATCGAGAACAATGATAATCTTGATATTCAGGCAACTCAAGAAGCAGGTCAGCTTTCTTTAACTCTTGCCAAGATCGGTAGAATTCGTATTATGGATTCTTTTAAGAAAATTAGAGTAGTCTCTGCTGATCCTGAGAATAAAAGACTTGTCCAAGAGCTGAAAAAGGATAACGGGAAATCAATGTATCTCTATGATTTGAATGATGATTTCACCTATAGAAAGGATAAGTGAGCTTTTTATACCAAAGAGGGAATTCTGATTGGAAGTCTAAATAAGGATATTGTTTTTGAGCTGACTAATCAACCTCTTCTTAATTTCAAGGTATGGAACGTTGTTGGGAAAGGGCAAGTTTTAGGAAAAGCTATTTTTACGAATCTTGATTATAGTAATTCAACTGCAGTAATAGAGGACGAAACTTTTGAGTTTTGACCTATACTTGCTCATGGTTCAAGTAACAAAACTGCCAAAGCCATATTCTCTAAGCTCAATACCCTAAAATCTACCTATCAAGGCTATGACTCAATCCAAAATAGCGATGAAATGGAAAAGAATATTGGATTTAGGGGAGAATTTAAAAATGTTACTTTGTTTGCTGGAGGTAAGACCGTTGGTGAGGCTACACTCCCTTTTTGATCAGAATTTTTGATCAATATTTGAGATCCTCTCCTAAAAAGGATTGATCAGAATGCAAACCTTGATAATGCTGACTACAATGAAGGACTTGGTAAAGTGATTTATCACAATGATGAAAAGCCGATATTTAAGGTTATTGATATTGATTATAATAAAGACTGATTAAGGGACATTTTGGTGGTCTACAAAGATGGGACTATTAGGCTTCAGAAACAGTATCAGGATAAGAGATTTCGTGATATGGAGACTTTAATGGTCAGCGCAGAACAAATTAAGGATATTTTTGTTGGTGATGTAGATGGGAATAAATATGAGGATATTATTATTAGGAATACCAAGGATCAACTCAGAATTTATACTAATGAAAATTGAGTTTTTGATGTAGATGGGCGTCTTGCCTGTTTAAATACCAATGTAAAGTTAGGAGAAGTCTCTAAAACACCACGATCTTTAACGGGGACTCATCAAATTTTTGTCAAAGATATGGATTTGGATGGGAAGATTGACATCTCTACGTTTGATGTTAGAGGATATCTAAAGATTTTTTATGGGAATTGAAGCAAAAAATCACATAGTTATCTCAGTAATACCGGAAGTACTTGTGATGATGAACGGTTTAAGAGACAAGAGAAATCTACAAAAATTGTGAAAAAATTCTGACTTCAGCTTGGCAATACTCCTGTAAAAGATAGAAGCATCATTCGTCGAGATGGTCTTCGCTACCCAACACAAGAAGAATTAAGTAAAAAAATTGAAGAAAAAAATAATAGAGAAATCTGAGTAAACCTAGATCCTATTGTTATTTGAAAACTAGAAGATCCTGATCTCTGTCCACCATGTGTGGTTACAGACTATGCGTGTAGTACTAATGCTAATTGAGAAAAGGTTTGTAAGAGCTATAAACGTAAAAGTGCAGAACCTACCTGTACTCAGCCAGCAGATAAACAAAGCGATGTTTGCTGTGCTCAAAAGGGTGAAGGAAGTAAAGAAGAAAAATGTAAACTCTCTAAATGACGTCTTGACAAAAAGACTGAACAAGAAATTCATAGATGAGATACCGATTTTGACTTTACTGATATGGCTCAAGAGGGATTAAGAGTCTTTGATAAGTACGTTAAGAATCCATTTGACAAGATTGTAAGTTTTGATGATGGCGTTAAACCATCAGAGCAAGCCTTTTTGACTATTAATCAAGTTGATTCAAAAGATCCAATACTTTGAGTAACAAAACTTTATCAAGATCTTGATGGTGGGAACCTTGAAAATTGAGATACTGTAAAAGTAACCGTTACTATTAGCAATAAAGCTGCTCATTTTTGAGCTGGTGGTGCGTTTTTTGATCGTATTCAATGACCTTGGATTATACATCAAGATCCTAAAACAGGAGCTCCAGAAAACCTAAAATTTATTGAAGGAAGTGCTACAGTTCATCCTCAAACTAAAGAATATGCTTATTTTCTGCAAGATCTTCAATTCGCAGGACAAAGAATCGTTTATAGCTATGATTTGACCTATCAGATGAATGATCCATTATTTAAAATCAGACTTGAAGATGCCGAGATTACCGATTATAGCGCACCTCCAAAAATAAAGAGAACTGCACCGAAAGATGGTGAAAATGCCGATAAGCTGCCAGATTTTATTGTTCAATCTGTGGATGGCTGTAACAAGAAAGAATCTATCCTCTTTAATAATAAATCTGATGCTAAGAGGAGTTATCGTGAGTCCGTTATCGATCTTCAAGAACTAAGTGATGTATATTCTAAGGCTATTGAAGAGAATAAGACTAGGACAATGGATAAGGCGAAATCTGACATTCAAGACAGTAAGACTGTGGAAAAGATGAATGCTATCCCGTGAATAGAAGATATTCAGGAGCATTACAGCAACAAAGATATCTTAAACGAGTTTTGGAAGGCCATGAAAGAGGACTCCTCTGTTTCTGTAGAGCTCTTCTCAGACGAGACCTCAGAAATTGAAAAGACAGTGCAAGATATCGCAGATAAAGCATGTAATGGATTCAGTTTTGGACTTTCTAATGTTGGATCCTGTGAAGTTCCGGTGCCATTTAATCAGGCATTCTTAGCTCCTGGGAAATATCATCTGATGTGATGTATCCCTCTTGAGCCACTAACAAAATCCTTGTGAAAGGGGCTTCCTGTTTTCCACTTTCCAGGGACGCTTTACACGCCGATCTGATCTCTTCCAATTCCTTGGGGATTGAAGTCTCCTTCAGACTGATTTCTTTGGCTTGGATGAGGAACCTATCCTTCAATGATCAGAATTTACGTTGCACCTACACTCACAGCACAACTAGGGTTTGCAATCTGTTTTTGACCTCATTCGATAGGGAAGAATCTACCTTCTCCTTTTGCTGATATTTGAGGGAACTGTATCGTAATGTCGATGCCTCTACCTTGTGGGAAAAACCAGAAAAAAGACAGTAGCTGAAATAAAGTCAAGAAACCAAATGATAAGGTAGAAACCTACCATCCTTGGGCAGGCGACTATCAAGAGACCAATACTTGCGAACTAAAAAAACAAGATTCTCCTTTTATTACCGTGGCATCAGCACCTGTAAACTCATCGAGCCCAAGCAGTATTCCAGAAGGTAGTTATCTTGGATGATTTGTAGATATTAGCTATGATAGTATTACTTCTGAGGCCGATGCTGACCAAAATGGAATTTTTTTTGAGGGAGTAAAGATTCTTGGCGTGAAAGATGTCAATAATAAGGTTCTCTGAGGAAAACAGCAAGGAATTAAAGCTAAATTTGTTGGATGGTTTGATAGGCAAATTCAGTATTGGATCAATAATCTCTTAAGATTCCGTGTTGAGTTTATTTTTCCTGATCTCGAATATCTTAGTAAGCAGCTCTCTACCTTAAATCCTCAAGAAATTTGAAAGCTGATCTGAGGAACTGGTCAAGAAGGGAAACAAAATATTGATAAATTCCTTAATAATATCAAAGATTCAAAGAAAATAAGTTGATTGGCAAAAAATACATGGTCATCACTTCAGTCTAAAGAACAACTTACAGCTTTGGATATTGCGAATGGAGACAACCCCTTTGAGAAACTTCAGTCTTTCTTTAATAGTAAAGAACTTGTAAATATTAGAACCCAAAAAATTCATGTCAAAATTCCTTGGATCTATAGCGAAGATATTGAGGCATATAAAAATTACTTGCTTACATGGATGGAAACGAACGGAGAGGTTTTGAAGGCCTGGGAGTGAATTATTGAAAAAAGCATCTCCTCTTGCCAGAGACAGCAACAGGGAACCTGATCAAAAAAAGCTGATTGTTCAAAACTCCAAACTGCAAAAACTCAAATACTCCAAATGCAGACAAAGCTTGATAAAACAAGTGAACAGATTTATCAGAATATTCAAACTCTTGAGCTTTATAAGAGATTTCCACTAGAGATTTATGAGTGGCTTCACGTATCTGATAGGTACTTAACTGAGATATCATCACTCATTTCGAAATTCTTTGGCTATATTAATTATTGGATGGATGTTAATGCCAATAGATTCTCTCAATATGTTGATGCTATTATTACGATTATTGCGGTTGTGAAGACTTATCAGGTAATGGTTGATCTTTTTGTGGATCGAGGAAAGAAATGTGGTAAATGTACGCAAGATTCTTACGATCAATATTCTTGTAAGCTCAGTATGCTTTGTCCAAATCTACCTATCATACCGATCCCTAATGTAAAAATCCCAAGTCTCTATGTCGATCTTTCTCATCTCAATCTTGGATTAGATATTGTGCTTCCTCGTTTTAATTTTGTACCAGAATCTATTGAGCTTCCAAGACTTCCAAACCTTCCAGAGCCGCCAAGATTAGGGCTTAACATTAATGTCGACTTTAATATTCCAGATATTCCTCAACTTCCTGAGCCTCCAAACTTACCAGAATTGCCATCATTCATTCCTCAAGTAAAAATGGAATTGCCGATTCTTCCTCCTGCTCCTAAGATTCCTGAATTGCCTAACAAAATTCAGAAAATTGTCAGTATTGCTAAGAAAATCTCTAAGATTTACTGTATTATCAAGTCATGAATAGGTCTAGTAGGAGAAAATTCTGTAAAGGCGAGAATTGAACAGATGACCCAGAGAACGTACGAAGTTCCTTGGGTTGACAACCTTGATCTTACTCGTCTCTTTAATCAACAACCGCTACAATGAATTGATATTGAGGTAAGTACTTACGTGAATTTACAGTATAATTTTGATGCGTTCTACGCACTTATGAAGGGTATTGTAAATAATGTTAACCAAAAGACCAATAATCTCGTTTCTCAAGGGCAAGAAGTCTCTGATGCTCTAAGTACTGCGAGTGATACCGCAACAAATACTTTAAATTCAGCTACAAGTTCACCAATTCAAGTAAATGTTGGTTATCAAAAGCAATTAAAAGATAGCCTTAAAGACGTTCAAGCAATTCTCTCACCACACCAGGAGAAAGAAAAACTTGATTCGATTATTGCTCTTGCCTCAACTGATGGCGAAGTAAAGAAAGACTCTGATTGACTAAAAAAAATCCAAAATCAGATTTCTGATCTTATTCGTAATGAAAGAGAAGACTTAGCAAATCTTTCTATGCTTGCAAAGTCAGACTACTCTACTTTTCTGAAAACCTTGGAAGGAGCAAGTAAAAAAGAACCACTTCAGCTCACGTTTTCTAGTAGCCTGCTGGCGGAAAACAAAGGTGCGGAACATGCGATTGCAGGCATAAATCCTACTGAAATGATTATTCATACGGAAGGAGAAAGACTTCAGGGTTATCTCTCAGCATTAAAATCTCATAGTCCAGAAAGTTTGAATATGTCGAGTGAGAATTATAATAAATCAAAGTCCTATCTTGAAGAGGTCCAATCGGCTCTTAATCAGCGAAAGAGCATTAAACAGAAAACTTATGCAGGAAATCAACCACTCCTTACTAAAAATGGCACAGAAACTAATAGAACGCTTTTGACGCAGACTTCTCAATGAAGACCTACGACTTTTGAAACTGCATCAACGGATTTGAGTAGCTATATAAATGGGATCCTTGTGAAAGATCATAGCAATAATATGGTAAATGTTGTGCATTCCAAGGTTAACTATGAGAGGTTCTTTGACTATTATCAAAAAGATATTAATAATGATAAAAAACCTGAAGTCATAACTCGGGATGAATTTAATGTCTACGTAAAGTATGCAGACGATGTGGAACCAAAAATGAAACAGCAGTTTACAAAATATTATGTTTTAACTCCAAACCTAAAAAATAAAAATAAAAAATACGAATCCTTTGGAGGAACACTTTTCTGAGGGGGAGAATCAGTGAAACTTTACGATCAAAATTGGGAGGTAAAGAATTTTAGACTTGACGGTCAGACCTTTGATACGATCAGTTTTTCTTGGGATAACAATAAAACGGAAGATATTGCCTGATATCTCGTTCGTTTTTCTGAACGTGTTGATGGATTATTGGAGAAGAATGACCTCAAAAATGTAAAATATGCCCTTTTCCTACCTAATGAGACTCCTCTCGATCACCTTAAGCTGAAGGCTGATGATATAAGTTTCTCTGTAAAAAGTCAGATTGGAAAGCTAATAAATGAAGTTCAATACTACAATCCTGCAAATGATGTATTCCAGTTTCAGCTTCACCAGCTTCCTAGAAAATGGCAATACTTACAAGTTACTACCTTAAAGAAACAAGCTACACTTTATGAGCAAAATGCTCCACGATCTAATCAAGTTGTAGGATGAACTCAGGTTATTTGTGATTCAGAGCCTCCTAAACCTAAGGTTGAACTTATTAGAATTAAGAAACCTGTTGTAGAGGACGAAGGATTTGATCTGAAGGGGTTTATTGGAAGCTATTATGACCTTCAAGTTACACGAACAGATAATCATATGGTTCGTCGGGCAAAAATTGAAGAGAATGGGAAAACGCTTGCTCAAAAGGATATTAATGCTCCTAGTGGCACGATCACATTGAAAAACCTCTTCTTTACTGAAGCTCAGGTGAGAAATTATGTAATATCAGCTATTGATAGTCAAGACAATGAGAAAGAAGAAGAGGTTACTCTCGAAATTAATATTCCTAAGATTACCATTGAGGATATTCAGCGTTTTTCTGGATGGAAAGAAGGAATTGAAAATCCGGTTTTAATCTCTTCAGCACTAGAAAGAGATATTGATAAGTGAGATGTTAGCTTTGAGAGGAAAAGAGAAAATAGAGTCTCAATGCTTACTGCGATTTCCAATGGACAAAAAACGCAAGTTTATCCAGTAACGACTTATCAAACTGTTGTAACTGGAGCTTATTATGATTTCTGAGATCTGATCGGTCTTTATACTACTGATAAAAAACTGATTGCCACTGTGAATGGTCATAATGGAGAAATCATTGTTCAGCCTGCATACAAGAAATCTGTTTCTCTAGGAGTAGACTTTTCAAAAGGCTATCCTGTTGTAAAGGTAGTGAGTGATGCCAAAGTTCTCTTCGAAGTTGTCCTAAAATCAGAGCAACTCCTTAGCAAAGAAATCTCTGATGGAACACTCTTCCCTCTAGAGGGAAAGGATTTTGGCATATTTGATGGTGGAGAGGTCGTGATGAAAGGGAAGGAACCACTTCTCTATATTGCTCCAGACGGATCGCTTCGAAGTAGTAAGCCACTGCAGTGAAGTTATCATTTTGACTCAGAGCATAAAGTCGTGACCTACACCCTTTCAGAGAATGCCTTTTGAAAGCCATTTGCTAGGATTACATTTAAGGCAAAGTCGATATAAAGATTTGCAAAAAGTGCAAAAAATCTGTATACTTATTCTGATTATCTTTAGATATAAGGCTATACTGAATGAAAAAATCAGTTTTCACCTTTTTGTTTGGGATTCTTCTCCTGTTTGGAATCACTTCTGCACATGATTGCCATCTTTTTCCTGAACTCTCAGTAAAAAACCTAGAAAAAACGTTATTTGAAAGCTCAGTAAAAGGAAATCCAGCATTAGCGACACTCACTGAGGAAGGATTACGTAGGGCTCTTATCAATTTGAAGGCTCATTGTTGCGCATCTCAGATTTTGGGTGATAATGCACAAATGCTTGCTTCCTGTAAGAAAGATGCAAAATACCTAACTGAAAGAACAAATTACCCTCAGTCTTCTTTTCTTTTTGATCATGTTATTGATGTGATGATGAGGAGACTGATGGTAGACGGGAAATATGAAGAACTCCCTCTTGACAAAAAAGCTCAGGAATGGAGAGAAAAGACTGATCGTATCGCTGATGGAGCGGATGGAGTTATTCCAGTTGCGATTTCCAAGGATTATAAAAAATTTTGGGGACTTCAACCTGAGTTTCTTATTCCTGAATATAATGGGGTTTCTGCTCCTGAATATAAAAAAGCGATTAATGATTTAGAAAGCAAGAAGGCAATTTTTGCCAAATATGAAGATTGGAACTTAAATACGAGATATTATAATCTTTGTCAAAGTGCGATCTATTTGATGACTCATCTACCAATAGATTTTTGAAGTGAACAAATGGCTCTTGCACAGAAAAAATGCTCCTCTATGACCCAGCAAAATATCGTAAATGAAATCACCAATCTTTCAAGACTCATTTCTCATAAATCTGAGCTTCTTTTGGCACAGAATATGGAAAACTATGCAGATGCATATCTTATGGACACAAGATGAGGAAAATTTGAGGAAAAATCTCAAAGGATGCTGAGTAACCTCTTTGGAGTAATGAGGATGGTTCCCAAGCTTATTTCTATGTGTAATTAATTTACTTTTTGTCTGATCATGATGAAGCTAATTCTTAAGTTGTTTGTTTGTTGAATGCTCTGTATATATGGTTTTATGAGTCCTGAAGTCTGACTTCTTTCTTTTGCTCAGAGTGCTTCGACTACGGCAAAAACGCTCCATATCATTCCTGAAAACTCTGAAAGTGATGAGAAACAGGTAAAACAAGATGTGAAAGATGTGGCAAATAGAACAAAAGGAACTGTAATTGATACCTATAATGAAATTGCTAGTGGATACGAATCTAGAGGAGAAATCGGGAAAGCTATGCAATCCGGGATTATGGGTTGGAATACCTTGATTCAGTATGTAGTTTATGCTATGAGTTTTCTTTCTCAATTAGGACTGCTTATTGGGGGGCTTATGATTCTCTATGCAGGATACCTTTATGGATCAACCGTTTTTTCTGGAAGCAATCCAACAAAAGCAAAAAGTGCAATACGTAATGCTATTATTGGTGTTTTGGTTGTGGTCTTTTCTTTTGCCATCTGGAAAGGACTTGTGAGCAGTTTTCTCTAGGATAATCAGGGGTTACTCTCATGAAATTTCCTTGATTTGATCACGGATGAGTGCTGCTTTTTCAAACTCTCGTGCCTTTATGGCGTCATCAAGCTGGATTTTAAGGTCTTTTAGGATAATTTCTCTCTCTGCTTTTGTTGCCTTTTTCAGCTTTTTTACTTTACCTTTGGTGATACTTCAAAATGATTGATCTAAGCTTAGGTCGTCTTTTACGGTTTCTAGAGATTTTACATTGGAGAGTGCGGACTTTGGAGTGATTCAGTTTTTTGTATTGTAAAGTTCTTGGATTCTACGACGACGATAGGTTTCTCGAAGTGCTTTGATCATAGATTCGGTAAAACTATCTGCATAAAGCACAACTTCACTCTGTGGATTTCTCGCTGCACGTCCAATAATTTGAATTAACGAAGTCGTTGATCTTAGAAATCACTCTTTATCTGCGTCAAGAACCCCGATCATCGTCACTTCAGGAAGATCAATTCCTTCTCTAAGGAGATTAACACCGACAATAATATCAATTTCTCCTGTTCTCAGCTTCTTAATTATTTCCCAGCGGTCGATAGTAGCAATCTCACTATGCAGGTAGAATGCTTTGAAACCCTGAGCTACGAGAAAATTTGTAACTTCTTCAGAGGACTTTTTGGTCAAAGTAACAAGGAGAATTTTTCCTTGATTATTGATATGCATTCTGATTTTCTGCAGAAGATGGTCCAAAAAAGGGGGAAAGTCAGATTTTTTGACTTCATTTAGTTTTTTTTGGAGATAGGTTTCAATCATATTGTGCTACACTTAAAATGTTATTCTCAGCAAAGAATAGGTATCTCTACAGAAAAAGCAAGTGAGTTTTTGGAGAATAATGTCACAAAAATCTAAAAAAAACGAAGAAAGAGAGAATACTCTTGATAAATCCTCGCCGATGCATACAGTAAGAGAGTGTTCTTTATTTTATGAATTAGGTATGCATCATAGTGACCGTGAGCTTGTCCTCGCTTTTCAAGCTGGGAAGTATCATTGCTTTGAAACTTTATATGAACGTGTGCTTCCAAAAGTTTACCGCTATCTTCTCTTTAAAACTAATGGAAACAAAACACTAGCTCAAGATCTTTGTTCCGAAACCTTTTTGGCTGGATTTGAGGGATTGGGAAGTTTTCAAGCAGATGAGAACTGTAATTTTTGAGCTTGGATTTTGAGGATCGCCCATCATAAGTTTGTTGATCATATTAGAGCTCGTCAAGAAGAACAACTTTCTCTGAACGAAGAGTTAATCGTGACGGGAGAAGATCAGCTTTTGCAATCTTTAGATTTAAGTATGCAAGCCTGAGTTTTCCTTGACTTCCTAGAGACGCTTGGTGAGGAGAAAAAAGATATCGTAGTAATGAGAATTTGGGAAGATCTTAGTTATCAAGAAATTGCTCAAGTTCTCGGAAAATCGGAAGAGGCCTGTAGACAGCTTTTTTCGAGGACTATGAAGGCTCTATGCGATCACTTTAAGTCATAATTTCCTTTAGCTCTTATTCTTTTTTTTGATGAAACTAGATGAACTTCTCAACGAGCATCCTGAACTCAGTAACGAAAAAAAGCAGATTGAAGCTGTAGTTTCTGCATTTGCTGGGAGTAAAATAACTATTGATAAATCTTTTTCTCAGTCTCTTAAATCATCAATTCGCGAGAAGATTAAAGAAAAAAAGGCTCAGAATCCGAATTATTTTTTGTTTTATCTCCGCCATTTTAGGTTTTATTTTACGGGATTTAGTGTAGCATTAGGATGTTTTTTGATCCTTTTTGTGCTCGGATTTTTCAATTTTAATGCTAGGCCTCAAATACTTAACAATCCAAAGCTTCTTGCTTACGTTGGTCCTGAAGCGTTTTGAAGGTTAAGTAGCCCGACTACCAATGCTGGAGATCCAGAAAGACTTTCTGTTGCCAGCTTTTCTGACCTTCTTGAAAGCACTTCTGAAGTATATTCTGGGGATGAAACCATCCTCACTGCTGCCAACCCTCGCTATCATATTGGTGAAAAACAGCTTCCAAAACTTCCTTCTGTAATTTTAGTTGGAAAGAATGTGAATAGCAACTCTTCTAATATGCAGTTTCTCTCAAAGAATATTAAGCTTCCTGAGTTTTCTCTTTCTCCTTTTAAAAAGGACACCATTACGACCATTGATTTTGAGAATAATCAGCTAGATTATCATTTTACGATCAATCTCGATCAAGGGCTTTTCTCTGCGATAAAAAATAGTGCTTATAATAGTTTGACTGGCTCTGATGTTCCTTTATTATCTGAGAAAGAAATTAAAAAAAGAGTCAAAAGTCAGATTTCAAATTTTTGACTTTCTCTAAAATTCTATGGAGAACCTGAATTAAAACAAAGCGAACATGATTCTCATACTTTTGAACTGTTCTATCCAAGAGTTATTGATGGGAAGGAAGTATGGGATCGAGATACTAACACAAGACTAGGAATGCAAGTTTCTTTTGACCTTGAAAGTCAGGAAATTATCTCACTCTACTCTTTCCAATTTCAGTCCTATGAATTATCCAAATATCCACAGACAAAATCTCCATCCGATATTCTAGAATCTATTTCAAAACTTGGAAATATTAATGTCTCCAAAAATAAAGATGAAGGGGATATTCCTATGAACAAAGGGAAGGAAATTTATCTCCAGAAGTGAGATTTTCTCTTTCCTGGGTTGCTTTTTCAGGCTCAAGATGCTAAGATTAAGAAGGTTTTTCTTGTTTCTCTCTATTAGGAAGATAAAAGATTAGGCATTTTATGCTAAAATCCTTTGACTTTTGAGGTAAAAATCTTACTCTAAAGTCAGATTTTTCTTTTTTTATTTTTCTCATCTTTGCTAATGAAAAAACTTCTCTTTGGTTTTTCGGCGTTGTGTATGCTCATTTGAGTAGGAGCAAATGCTGCTGATACCCAGCTCTTGGAGCAGCTTTCAGGTCACACTTTGGATGCAAAGCAATTAAAATTTTCACAGGTAGAAAGCTGCGAATCTCTAGAAAAAATGCTCGAGAAATATGCAAAAGAGACTCAAGAACCTCTCTATAGAACTTTTGGGCTCAAAAAAGAACTTGCTGTCGAGAGTGCAGCACCTATGGCTCAATGAATAGCTGCTGAAGCTGCGATGACTAATAGTATTTCGAGCTCTCATACTGATTTTTCTCAGACAAATATTCAGAAAATCTGAGTAGATGAACCAGAACTGCTCAAGACAGATGGAACCTACTTTTTCTACTATAATGAAAAAACTCAAAAAGTCAGTATTGTCGCTTCTCCTCTTGATAGTAAGACCTCAACCTTAGATGGAACCAGGATTCAGCTGGTAAATGAAATAGCGGTTCCCAAAGAACTGAATGACATAGAACTTTTTGTGACTAAGGGACGTCTGGTTCTCATGGGACAATTGTCTGATTATGGAGACGCTGCGTATGCATCTTTTCTTAGCTATCAAAATCGTACCGTAGTAGCGATCTATGACATTTCTGATATAAAAAATCTCAAGCTTCTAAAATTCGAGAACTTACCTGGAAGTTATCAAGATGCTAGGCTTATTGATGATCAACTTTATGTGATTTCTGAGCTGAGGTTTAATCGATGGAACTGGAAAGGAAAGTCTCATGACTTTAGGAAGGGACTTGCTTCTGTAGAGATTGATGAACAAGGAGCAAAAGTGCTGCCTATAGAATGCTCTCAGATTTCTTATGTTCTGCCAGAAGATAAAAACCTGAGTCTTGATCCAGTTTTTACTCTTATTGCAGGAATTGATATTCGTAATACTTCTAAAAAAGTTTCTACCACTGCCCTTCTTGCGCCCAATGGCGAAATCCATATGAGTAAAAATGCTCTCTATCTGGTTTCAAACTTTTATACCTACAATAATTGGCCATGTCCTTCTGGACTTTTCTGTGTGATGCCGGTTTTCAGAAGCACGACACAAACGATGATTCATAAATTTAGTAGGGATAGATTGAATTTAAAGTATCAAAACACTGCGATTGTCCCAGGTTCCTTGCTGACTCAATACAGTATGGATGAAGATAGTGATGGAAACTTTAGAATCCTGACTAAAGTCTGGGAAAAACATCTTTCAACTCAGCTTTATGTCTTTAAACCAGATCTCACACTTGCTGGGAAACTCGAAAACATTGAGCCAGGAGAAGAATTTAAGGCATCAAGATATATTGGAGACAAGCTTTATTTGGTTACTTTTGAGCAGATCGATCCGCTTTTTGTGGTTGATCTTGCTAATATTAGTCAGCCAAAAATTATTTGAGAGCTTAAAATTCCAGGATATAGCACCTACCTTCATCCTCTCAAAAAGGAGTGAAGTAAACAATATCTTCTTGGACTGGGCTATAATACCACTCAAAATGAACGATGAGGAACTGTAAATTCTGGAGTTAAGATTTCTCTTTTTCAGGTTGATTATGCGAAGAAGCAAGGAGATATGATCGAAGTCAAAGAACTTGACTCACTTAACCAAGGAGGGAAAAATTCTGATTCTCAAGTCCTTTCAAATCCAAGACTCTTTGTAATGGATAAAAAAGGGAATGTTACTCTGCCTTTACATCTCGTTGAGCGGGCTCATCAAGGGGAAAAGTGTAACATTACCTATGATCTTCAGGGAAAGGAGCAGAAAAAAGACTGCACTCCTATTGTTGAACCTTCTGAATACTTTGTAGGCTTAAAAACGTTTTCTGTGAGTCCAGAAAAGTGAATTAAAGAACAGTTTGCAAAAGATTATCTCAAAGATATCCTCGCTAAAAAGTGAATAACTACTACAGGCAATAGCAAGCAGATTCAAAAAGAAATCTGGAATAATGAGGAAATCTTCTCGCTTCAAGATATGAGAGTAGGATATGCTTGAGATGCTTTATACTCATTTAGCAATCTTTTCGCAGATTTCATCTTCCCAAACAAAAAGAATTATTCAATCTGGTTTAAATAGCTTTTCTTGCCTTAAACAAAACTCTATAGTAGTTATTCTTCTCCTTTTATATTCTATTTGTGATTTACTTATGAAGAAAATTCTGATTTCCCTCGGCGGAATACTCTGTATCTTCTGAGTAGGTTCAGCTTTTGGACTGGATCTTAATAGCGCACTCACTTCATCTCAGCAACTCCTTAATGGAAAAGGAGAAATAAAAGTCAAAGCTGTCAGTACTGTTAAAGATGATGACGAACTTATTTTGAGGCTTAATAAAAAAGGGCTCACCATCCACAAAACTCGATCAGATTTTTCTCCTGATAAAGCTATTAGAAGAGATGAGGCTGCAAAGATGCTTACCTTAGCGCTCATCCACCTCCCTCAGCCGATTAGCTTTGCTGAGAATAAGGATTGTGTCTTCTCTGATGAGGATCAGGCATGGTCTGATCTTAAGGCAGTCTTGATGCAATCCTGTACAAAAGGGCTTTTTAAAGGGAATAAGGGAAAGTTTAACCCTCAGCTTTCTATTACTAATGGGCAGATTATGACGGTGCTCGGGAGAATGCTCTTTGGGCAACAAGACGAAGGAGAGGGGCATTATGCGACCCGCTATGTAGAACTTCTTGAACAGAAAGGTTATCTTCCTGATAGCTCTTTGAGCGAGGAAAAAAATCGAGACCTTCCTGCAACGAGAGCAACGCTTGCAAAATTACTAGGGAAGGTTTTGAAAGATTAGCCTCGGAATATCATTTCAAATTTTTAGAAAAAAGCTTGATCCCTCTTTGAGTTTCAAGCTTTTTTGTCGTTTGTGGCTTTTCTGCTGAAGGGAGAAGTTAGCTCTTTGCCTCAATTTCTACTCTTGCATACAAAATTTCTGGCTTCAAGTCGACGGTAAACTCCTTGAGATTGAAAGCTGACTGCACCTGTGCAAGATTCAGATTTTTGCTGGTATCAATCTCCTGTAAAGCGTCAATTCCTAAAATGTTTTTTAACTTTTCAAAGCCAACCGTAAGTATTGGAGCGGAAAGTATGGCGAGATTTTTAACAATATAGAGCAAGAACTGTAAGTCCCTTTCAGCATCGGCTTTTGAAGACTCCTCTTTCCATTTTTTTCGAGGCTCGGCTTTGGTGATATACTCGTTTGCTTTTTGAACGATACGATACCAATCCTGAAGGTAGCCTTGAAGATCAAAAGTCTTGAGATAACGGTCCTCAATCTTCTCAATGAAACCTTCAAAATTCAAATCTTCATCGCTTTCGTTTCGTGATCTTAGGAGTTCAGGATCAGCTTTTCCCTGATTAATCCCATATTTACTACAAAGGCTGACTACTCTATTAACGAGATTCCCCCAAGCACCGATCAGCATACTATTATAAACATTTGCGAGCCTCTCCATAGAGAAATCTCCGTCTGCATTGAGAGAAACATCATAGAGCAAATTGAATACAAAGGGATCTCTGCCATATTGCTCAAGAACCGCCTCTGGATCAAGGACGTTTCCTGTAGACTTGGACATTTTAGCACCATTTAGGGTGAGATGCCCGTGGGCAAGCAAGGTTTTGGGGAGCGGGAGCTTTGCAGAAAGGAGCATTGCTGGCCAGAACGCTGCGTGGAATCTCAGAATATCTTTCCCGATCACATGCACATCAGCTGGCCAAATCGCTTCTCGTTTATCGCTCAGTCCAAAGCCTTGTCCCGTCAAATAATTGGTCAAAGCATCACATCGCACATACATCACGTGATCCTCATCTCAAGGCACCGGGATTCCCCGAGGCATTTTAGCCTTTTGACGAGAGAAGCTGACATCTTCAGCCTTTTCTAAGAAGGCGAGAATCTCGTTTTTTCTCATTTCTGGTTCAATCTGATATACGCCATCCCTGATCAGCTTTGCTACCTGATCTTTATAGTTTGAAAGCCTGAAAAAATAGTTCTCTTCTTCTATTTCTTGGAGCTCTTTATTGGGATGATCGGGGCATTTTCCATTGATGAGGTCTTTTTCGAGCTTGAGTGCCTCACAGCCCTCGCAGTAGAGCCCTTTGTAAGACTTCTTGTAGAGATCTCCAGCTTCAACGAGCTTTTTTCGCATCAACTGAGCTCAAGGGTAATGTAATGCTTCATTTGAAGTCTGCTGGAAGACATCGTAGGAGATGTCGAGCTTGGAATACAAAGACTTAAAGAGCTGCGTATTCTTCTGGACGAAATCATGGACCTCCAGTCCTGCTTCTTTGGATGCATTTCGGATTTTCATTCCATGATCGTCGCTCCCCGTCTGAAAAATCACCTCATAGCCGAGGAATCTCCACATTCTTGCCAAAGCATCCGTCTGGATAATCTCTAAAGCATGCCCTGCATGAGGACCTGCATTCATATAAGCAATCGCTGTAGTAATATAGAACTTTTTTTCCATTAAGATACTATTTGAAAGAGATAAACAAGCAGAAGAGACACCTCAACTACGAAAATTTTGAGAAGAAATGAAGGAGCAGCCAGGATTATTTGACCTCTACTGGCTCGAGTATGACGTCTTCACTGTCAGCGCCCATATATTTAGAGAAATCATCTCCTCTAAAGAGCTTTTTCTTGATAGTATAGCGCGTACTTTTAGCTACGTCTACGTTATCAGGGGTAGCTTCGTCCAATCCGTAATCAACTGACTCCTGAATAAATACATCTGAGAGGAATTTTCGTGGGCATTTGTCAGGTTTCCCTTGATCATCTTCACAATCATAGGTTGAACTCTTTTCATCAGCTTCTGTTTCGGTAAGATTTTCTCAGTAGATTTCATTGTACAAAACGTAAAGTGACTTGGTAGGATTGATAATTGAATAGTCAGTATCCAAAAACTTAGGAGCTTTCTTGGTAAATTTTGTCGTTTCTCCAAGATACTCTAGGGTGTGCTCTCCCTTGGAAAGCTTGATCACCTGAGAAGTTTTTGGCTCTAGGGTATAATCTTTTCCATCAAGCTTGAAAGTGATCGCTTCCTCTGTTGGATTATCAATCACTGACTCTGCACTCTTGGAAGAGCTATATACTCGTGCTCCGATCACTACAAGAAGGATCACTGCTAATGAGGTTCCTGCCTTGACAAGCCAAGCTTTTGCTCCTGCTTTCGCTTCGTTTAAAAATTCATTCATTGTGGTGGGGAAAAAGAAATAAAAATTGGTCATAAAAAAACCAACACTGATGTATATACTAATATTTAGCTTGAACACAAGAGATTTTATATCAAAATCTGAGATTTGCGAGACTTTTTGCCTCGCTTCTGCCTGAGGGAAAGCGTCAGACTTTTAAAACTTTGGAGAGGAAGGGGAAAAACGCTATGCATCGTGCAATACTTTCCCTAAATGTTACTCAGAATGGGCTTGCTTTTCGTGTTGAAATGGCTAGTGTTGTCTTTAGTTTTATTTTCTTTTTTGAATGCCGATGAAAAAAGAGGAACTTTTGAAAACTACCTTTATCTGCACGACGATTTTGGTCAGTGTTATTTTCTTTTGAATAAATCTTTGAAAGATGAGAGCTCCTGCACCAGTCATTTCTCAGGGAGAACTCCAGCAAAACGTCAACGCACTGAGCGTGCAAGGGAAAGGAGAGGTATATGCTGCTCCTGATATGGCAACCCTCAGCATTTCCCTTTCAAAAACTGCCCCAACAACGAAAGAAGCACAGAATTTCGTTGCGACTAAGATGAGTGAGGTGCAATCACTGCTCAAGAACTTCCCGATTAAAGCCGAAGATATTCAGACGCAGAATATTACGATCGGGACGGAGTATGATTATGGGTTTGGAGGAGGAGAAAGGAAGGTAAAGGGCTATACTGCCACCTACGCCATGGCTCTCACTCTTCGCGAGCTCGATGCCAAGAAGGCAAAAGTGCTAGAAGATCTCATCAACACCCTCTCTGGTATTGAAGGAGTGGGTATTGAAAGCCTCAGCTATGATATTAGAGATAAAACTGAGTTATTTCGTCAGGCGAGATCGCTTGCCATGGAAAAAGCAAAACAGAAAGCTAAGGAACTCGCAAAGCTTGGAGGCGTTCGTATCGGCAAGCCTATAGCGCTCTCTGAAGAGGTTGATGCTACGCCATTTTTTGCTAGAAAGGCGATGGCTTCTCAGTCTAATGTAGCTTTTGCTGATGAAGCGTCCGCGGAAGAGGGAACTTCAACGATTGCGCTTGGACAGCTCAAGGTTTCGGTGGCAATCAATGTAAGCTACCCTCTTATGTAGGGCAATTGGGAAGAGAAAAAAGTTTTGAAAGCTGGGAGTGATGCTGGGTTGGGCTTCCTTTCAGCTTTTTTGGTAAACTTTAGCTCAATCTAGCACTACTTTGCACTCAGGTGGTGCTGGAGTGAGGCAAGATGAGAGGGGCTTGCGAAGTGGAGGGGAGATTTTTCCTTGATCTTTGGGTATTTTGATGATTTTAGTCAGATTTTTTGTTTTTTTTTGAAAATGTAAAGTCCTATCTTTCGGGAGAGAGGAGAAAAAGTTGAAGTTTCAAGATTTTTGTGCTATACTTAGGAAATGATTTATTTCTTTTCTCCAAGCTAGCCATGGTCTACATTCCAGAGAGCCTTATTGATGAGATTGAGGAGCTAAAAGAGCTCTGAAAGTTTGACGAAGCCATCAACCTCGTCAACTCGATCCTGTCTCGCGACCCTCGCAATGAGGATGCTATTTTACAGATTGCAGATATTCAATACCGCAAGGGTGAAATAGGAAAAGCTGACAAAGCCGTTGATTTTCTTAACGCACAAAAGAAGCATAATGACCCTTTGGGGCTCTACATTAAGGGGCTTTTGGAAATGGAGAAGAATAATCGGAAAGAAGCAAGAAAATATCTCTCAAAAGCGATGGAAATGACCAATGGAACGAATCATGAGATCCTAAGATGTTATGGGCTTTGTGAGTATTGGTATGGGAACAGGTCGAAAGGGCTCGAGTATCTGAAAGATGCTTTCGCCTTGGACTGCAAAGATGCTGAAGTGATTTACAATCTGATCCAGATGTACATTCTCGAGCAAGAATACAAGAAAGCTCAGGAAATGATTAGCTACTTCAACAAGCATCAAGCCTCGCTCAAGTTTGTGGACAAGAAGCTTGACTTCTATCTAACGAAGATCTCTTTGTTTGAGAAGTTTATCAAGGCGAAGAAGCTTTTCCAACTGAGAAAATAAATCAGACTTCCTTTATTTTGTATTGACTATCCTCTTATGAAAACGAAACACTTTGGATTAAATCTTATTAAATCCGACACGAAGACGTTGCTCAAGTCGTATCGCATTCCATTGATTCTGATTAGCATCCTGATGGGAGTATTACTCTTTGTCATGAATGTTTTCCTCGCTGGAGCGCTCTACGGGAATAGTTTTAATCTCCAGATGAAAGAGAAACTTGGGGTTTATCTCTATCTCAGCGATGCTGCTACTCAAGATCATACTGATGCTGCGCTTAAGTCTGCATTGGAAGAGAAAGGATTGAAAGTAGATTATGCCTCTAAGCAAGAATCGCTTGAGTTTGTAGAGAAAAGGATCCCTGATCTAACAAGCACCCTCAGGAAATACAACCTTGAGAATCCGCTACCTGCAACGCTCTATGTAAGGTATGGAGATCAGAATGAGTTTGCTGCGATGAAAGGAGTCCTTGAAAACTACAAGACTCAAATCCTGAATATGGACGATCTCTCTGACAATGCGATCAAAACTCAAGAAAAAAGAGTGCTGAACGTGATCAATCTCTCTAATTTTTTGCAGAGTTTTGCGTATTTTGTGGTGCTAGCGCTTGTGCTTACGATTATTGCTTTTGCGATTTTCTTCTTGAAGACGATCTTTTCACATTTTATAGAGGATATTCAGGCAAAGAAGCTCTTGGGTGCTACGTCTCGTCAGATTGTGCAACCGTTTCTGAATGTGATCTTTATGAGCTTGAGTTTTGCGTTCGTGCTTGCGATTGTTCTCCTCGTAGGTGGATTGATTCCGCTTGATTCCTATCTCTCTGCTCTCTTTGGGTTTGATCTCTTTGGGTATTTGTCAGGGTTAGCGATTGATTTGCTCGCGGTTGGAGTAATCGAGATTGGAGGAATTATGTTGCTCCTCCTCTGCATCTCTTACCGTTTCGTAATGAGGCTTCATAGGAAGTTGAAGTAAGAAGATTAGATCACAGTAGAACATCATAAAAGAAGAAAAAATCTGATCTTTTCGGTGGGGTGGATCGCTTGGATAGCGAGAAACTCCACTTTTTTTGGATTTATAGGAGGGACAGGAGGACGAGCATAGAGGAAGATTTGATGCTACTGGTGTGCGCGAAGGAGAAAACGCTAATTTTCTAACTGTTCCCGTCTGCGCGAGGGGTGCAACGATCATTCTCTAAGGGGGCTGGTCTGCGCGAGGGAGGCGAAGAGGAGGTTTACTGGAGGACGGAGGACACCCTTTGGAGGAGATCAGATTTTTCTGTTTTTTTTAACACGATAAGATATAAAAAAGGGAAAGAAAACTTGCTAAAATAAGAAAAGTTTCTATACTAGAAGGTAAGTTTTATCTTCCCTTTTTGCTATGCAACGAATTACCAAGCTTTTTCTCTGGATGATGAAAGCGCGCTACACGATTAGTGTGAAGCATTTTCCAAAGCTCGATCCAGAATACAACTATTTCATTCTGCCGAATCATATCGCGTATTTGGATCCTGTGCTATTGTGGGCGATGTTTTATCCGGAGAGGAAGCTGAGACCGGTAGCAACTTCGAGGTTTGCGGAGAATTTCTTTTTGAGGTGGCTATTTCGCTGGATTGGGACGATCAGCATCGTCGAGATGGCAAGAGAGAAGAAAGGATTAGCACCGAGAGAAGAGAAGCTCAACTCAGCCATGGAAGCTTTACAAGAAGCGTTGGCGCAGGGGGATTCGGTATTGCTGTATCCGTCTGGGCAACTAGCGGGGCAAGGGTTTGAATCGCTGTGAGGGAAGAGAGCTGCGTATCAGACGGTGCAAGGATTAGGAAAAAAGACAAGAGTTTTGCTTGTAAGAACCAGAGGGCTCTGGGGAAGTATTTGGTCGAACGCTTGGACAGGAGAGACACCGAGTTTGGTCGGGGGGATTGTGAAAGGGCTTTTCTATCTGCTAGCAAATGGGTTTGTTTTCTCACCAAGGAGGAAGGTTGAGCTGGAGTTTTGTGATATGACGGAAGAAGTCAAACAAGCGAGCAAAGAAGGATTAGAGGTTTTCAATCAGACTTTAGAAGCGTTTTATAACGGGAAAGGGGAAGAGAAGGTAAAATACATTCCTCACTTTTTCTACTACAACGATGTTGAAGGGAAAGTGTTGCCTGCACACGTGAGAAATTCTGTAGAGGATCTCCAAGCAGTGAATGGCTATGAAGGATTTGAGTTTGCTGAGGAGGTCGTAGCGACGGTTTGTGCCGAGCTAAGGAGGATTAGGGAGTTACCTCAGCAAGCAGAGATTACACTAGAAAGTAATCTGATTTTGGATCTGTATTTAGATTCGCTTGATATGGCGGAGATCAAGAATTTCATCCTGACTCGCTATGCAGGAGCATCAAACACTCCCCTTCTAGAACTGAAAACGGTTGCGGATTTGGTAGCGATGGCGATGGGGAAAACCGCAAATGAAAAATCTGATTTCCCTCCTTGTGAATGGGAGCTAGAGAACAAGGAGTATTGCGAGCCTCAGAAGGAGTTTGATGAAGATATGACGATTTTGGAGCTGTTTAGGAAGCAATGGAAAGCTGATCCGGATGCAACGCAGAGCTATGATGTGATGTATGGGATGCAGAAGAGGAAAGACATTGTATTGAAATCGTTTTTGATTAGCGACTATCTGAAAGCTATCCCTGGGAAGCAGATTGGGATTATGTTGCCAGCGGTCGGAAGTATGAATGTATTGCTCTTGGCAGCATATTTGGCTGAAAAAGTGCCGGTAATGATGAACTGGACGCATCCTCAAAGTGCGTTTGCACACTGTGTCAAGTTTTCAAAGACAAAAAAGATTCTGACTTCAAAAGCGTTTTTCAACAAGCTCAATATTGAACGGCTGAAAGAGTATGAGTTTGTCTTTTTGGAGGATTTATTGAAGAAGATTCCGCTTTATCGCAAGCTCAAGGCTTTGGCGAAGAGCTGGGTATTCCCATTGCCTCCTCAGTCTAGTGAAGATCCTGCGGTCGTGCTTTATACCTCAGGAAGTGAGGCACTACCGAAAGCGGTGCCACTCACCCACAAGAATCTGATTTCTAATCTGATAGGAGCATTGAGCATTATGCATATCAAGCAAGATGAAAGATTTTTCTGTTATTTACCACCGTTTCATAGTTTTGGGTTTACGGTAAATACAGTGTTTCCCTTGGTAGCGGGGTTTAGGAGTGTTAGCACGCCTGATCCTAACGATTCGCTGACGGTTGCCCAATTGATTGCTCATAGCAAACCTACTTTGCTTGCGACGACTCCGACCTTCTTGAGGAATCTTTTGAATGTTGCGAAAAATGAGCAGCTTGCATCACTGCGTTATGTGATTACGGGAGGAGAGAAATGTTCGGAGAGTTTATTTGAGAGATTTAGGCAAGCGGTGCCTCATGGGGTGATTCTTGAAGGCTATGGGATTACTGAGTGTAGCCCGATCATTGCGATCAATCCGATTGAAAAGCAGAAAGCTCAATCGGTAGGGATTGCTGTAGGAAATGGTGAGCTTTGCATTCTGGATCTCAACACGCAAGAAGTATTGAAGCAGAATCAAGAAGGGATGATTTATTACTGCGGTCCGAATGTGTTCTCCGGCTATCCCGATCAGAGCATTGAAAGCCCATTCTGGGAGCAGGATTGAAAGGTTTGGTATAGGACGGGAGATTTGGGCTATCGCGATAAGGATGGGTATCTCTACATTACCGGAAGGAAGAAAAGGTTTTTGAAGCTGTGAGGAGAGATGATTTCTCTGCCGTTTCTAGAAGGACTTCTCTTGGAGCAACGAGGGAATAGCGAAGAAGCGAATCTTGCACTTGAGGGGAAAGAGCATGAACATGGGATTGAAATAACGCTCTTTGTGGTTGATCTGAAGATTAGCTTGAAAGAAGTAAATAGCTATCTGAGATCGAAAGGGGTTTCCAATCTGATTCAGGTTAATCATATCAAGAAAATTGGTGCGATTCCACTCCTCTGAACAGGAAAGATTGACTACAAGGTCTTGAAAGGAATGATTGATGGGCATTAGGGCATTAGGGCTTTAGTCTCGTTAGTCTCAATACTAAAAATAGAAAAAAATCTGGGTCTAAACTCAGGTTTTTCTCTTTGAGGGAAGTGTGCACTCCCCTACTTTTCATTGAAATCCCTCTTGCTTTTTGAAAGGAATTCGTTGTAATGGCTTGGATTTTTAGCTTTAGGTTTTTGTCATGACGAAAGATACCGCATCTTTTAAGGCGAAGCTCCAAGGAGGCTTTTTTTGATTTTGGATTAAGCAGTGGAGAGCAACGTTTCTGGTGCTAGGATTGATCGTGATGTTGGGATTAACTGCACTAATGAGTCTCCCAAAAGAGTCCTACCCTGAAGTTCAACTCGGAATGGTTTCTATTGCGACGGTGTATCCTGGAGGGAATCCTCGTGAGATTGATCAGCTTATCACACAGAAGATTGAGAAGAAGATCAAGAATATGGATGGAGTGAAGAAGCTTACCTCGACCTCTCGCAAGAATGTTTCTTCTATCCTCGTCGAACTAACAGACAAAGTCAAGGCAACCGATATGAGTTCCAAGATTAAAGATGCAGTGAGTCAGCTTTCTTTTCCTGAGAATGTGGAATCTCCGACGGTTACTGAGATTGACACTCCTAAAGATAGGCTTTTTACGCTGCTCCTCTATGCTGAAAATCCTGCGATGGATCAGCACTACCTCAAGACCAAAGCTAATCAACTGAAAGCAGAACTGGAAGGAAAAGGTGCTATTGATACCATAGAGATCGAAGGAGGAAATAAGTATACTCTTGAGCTTCTGGTAGATAAACAGAGAATAGAAAGTCTTGGGCTTTCACTTACTCAGATTGCTGGGCAGCTTCAATCCTTTACAAGCAATCAACCGCTAGGAAATCATCAACTCTGAACGATGAACTATGATTTTAGGATTGATGGAGAACTTAAGTCTGAGGAAGAACTGCAGGCATTGCCGATTTTATTGGGTGGAGGGAAGAGTATTCCGCTTGGTCAGCTCGCGGACATCAAGAAAGTCTATGACGATAAAGATAGTATTCTCCGTGTCGGGACCAAAGAGAAAGGGATTGGAAAAGCTGCAGTTAGCCTAACGTTTAATAAAAAGGCGGGAGCCAGCATTCTCTCTAGTAGTAAGCAGGCGAAAGAAAGTATCGCAGCAGCTCTTAAAGATGCTGAATATGCTGAGCTGAGCTCTTTTTATACCTTGGATCTTGGTGCAGAACTCTCCAAAGACTACAACAATCTAGCAGTCAATATGCTTAGCACGATGCTGATCGTCTTTTTGATTGTGGGATTTTTTGTCGGGGCGATGGAGTCTTTTGTGGCTACGGTTTCTATTCCTTTAGCATTTTTCGTGACGTTCTTTGTGCTGAACTATCTCGGATTGAGTCTGAATTTTTTGACTAATTTCTCACTGATTATTTGCCTAGGAATTGCTATTGATACGGCAACGGTAATCATTCAAGGGGCAAGTGAAAATATAAAACTCTGATTCTCTCCAATGCATGCGGCGTTGCTCTCTGTGAGAACGTATAAAAATTCGCTGATTTCTGGGACAGCTACAACGGTTATTGTTTTCGTTCCGATGCTGACTTTGCCAGGGATTATGGGGAAGTTTTTGTCCTTTATCCCGATCACGATCTTTATTACGCTTCTGGCTTCACTCTTTATCTCGCTTACGCTGACACCAGCACTTTTTTCAAAGTTTACTAAGAACGGGACGAACTACACCTCAAGCTCAGAGGATGAAAATCTCCTCAATCCTGAAGAACAAGCGCTGCTTGCAGACGACAGATCAGACAAAACTCAGCTTGAGAACGCTCAAGCCTCTGGGGTTCGTGAACGCATCTTTTCTGCGATGATCTCGCGATATGAGAGGACGCTGACTCCCCTCTTGAAAAGTGCTAAGACGAGGATTACTCTCATTCTAATTCCGATTATTCTCCTGATCATCACGGTTGTGTTTGTGAGTCCGAGAGTAGGGTTTTTGATGATGCCCAAAACGGATAACGAGTATCTTACGATTGTTTTGTCTGCAAAGGAAGGGACTACTACAGAGACGCTCTATGCAAAGACCCAACAAACTGAATCCATCATTGGGAAGCTTCCTGAGCTAAGTAACTACAGTTGGAAGCTAACAGATAATACGGCAACTTTGAGCATCCGCATTGTGCCAAAAGAAGAGAGAGAAAGAGACAGCTTTGCTATTGAAAAGGAGATTGAAGAGCAGCTTGCATTCTTGAAGCAAGAGGGAATTACGGTGGAATCCAAGGTGCAAAGTAATGGTCCAAGTGATGGTGCTGAGATTGGTATCAAACTTACTGCCAGTAGTATTGATGAGCTTACTGAACTCATACGTGTAGTTGAGGACTTCAAAACTTTTCTCAAAGGAGTGCCGTGAACTAAGAATGTAAAGAGCACTGCTGAAGAAAGTCCTGGAGAGTTTGTCTTTACCCTTGATAGACAAAAGTTAGCATTGCTGAATTTGACGCCAAGGAATTTTGCACCCGAACTTTATTTTTCACTAAATGGTATCAAAGCTGGCTCTTTCACGGATAACGTTGAAACCCATGACCTCAAGATTAAATATGCAGACTTTACAAAAGAGGTCAGTCCTGACAACCTCATGGGAAGCTTTTTCTCGACCCAACAAGGGAAGGTTGCCCTCGCGAGTGTCGGTTCCTATAGTTTTGCTCCTGCGATTAGCAAGATTTCAAGGGATAACGGCACGATCACGATTTCTGTGCAATCAAATCTTGAGCAAGGGGCAAAGGCTGAAAACGTAAATGCTGCTCTCACACAATTTGCACAATCCTACAAATTCCCCAAAGGAGTCACTTATGCAAAAGGAGGAGAAACAGAAGATAATGCTGATCTCTTGAATGCGATGTTTATGGCGTTTTTGATTTCAATTATCGGGATTTTCGCTATTTTGGTGCTGCAGTTTGATAGCTATACTCAGCCGGGGATTATCCTCTATAGTGTGGTTATGGGATTTTTGGGAGCAACGTATGGACTCTGGATCGTTGGGATTCCCTATGGAATGATGTTCTTGATCTGATTTATTGCTTTAACGGGAATCGTAGTAAATAATGCGATCATTCTCATTGATAGCGCAAATGAGAACCAGAAACTCGGCTACTCTAGACCGCAAGCCATCAAGGAAAGCGCAAAATCAAGGCTCAAACCGATTCTCAGCACGACACTGACTACGGTAATCTGAATGGTTACCCTGACAGGAGACGGAATGTTTGCTCCGCTTGCATGGACGATTATCTTTGGATTGAGCGTGGCAACGATGATGACGCTATTTGTCATCCCAGCACTCTATGAAGATGAAGGAAAAATTAGATACCTCATTGCGAGAGTGATTCTGAAACCATTGGTAAAACTGATGCTCCCTACTACAGGACTCGGTATCGTATGGGGACTTTCCACGATGTTTGGAACTCCACTCTTTACAGGAGCCTTTGCTATGCCGTTTTCGTTAGCATTTTTGATCGCGGGATTTTTAATGCTCAACCGATACGGAGTGCAGTGCAATCTTCAGTGAACCCCATGAGTGGTGGAAAAGCTGATGAAGCTCCAACTTACTAATCAGCAAGGCACTGCTTTAAATAAAAAACAGCTGATAAAAAGAGTTTTCTTTAAGTGGGGGCTTTTGATTATGCCGGCACTCTTGGGAGCGGTTCTTTCTGGAGTGGCACAAGCAACGGGAATTGAAATGCTTTGAAGTATTGCTACAGCGATAGTAGCACTCCTCTACTTAATCATCTTACTTGGCAATCTTTATTCGGTCTGGACAAATGAGAAATATCAAACGTGGCGAGACAAACGAAGCGCTACGATGATGAAAAAACTTGATGATTAGCATTCAAGAGGCGGAAATCCTTTCAAGAGAAGGTTAACTAGGAAATAAAAATCTGATTTTTTAACTCTTTCCTCAATGCTTTATAATGAGGACAAAACTTGGCTACAAGAGCTCGGAAACTCGTCTGATGGTGCTTGGCTAGCAGGTGGCACGCCTCATGGGCAACAACATATTGCGTGAGCCTCGTCGGAAGATGCACGAGGGAAAGATTGAGCATAATGCGGTTATCATAGGTGCAGGATCCCCGTTTGGACTGCACTTTTTTGATCGTGAGCGGAATAGGCGGGAAGCCAAGCTCACTGGCAAAGCGTTGCAGGAGCGGATTTGCGTAGTCAAAGAGTTCTTGTTTGAAGAAGGCAGTTCGCTGTTTTTGGTCCTTGATCGGGGGGAGGTCCGTTAGCGGGATTCTCTCGCCAAAGAGCAGAACGCCCTCAGGAGAAAAAATCTGATTTTTTTGCTTTTGGTCGGCTTTTTGCTGAAGCTTTTCTCCTAGAGTTTGCATTTTCTGGAGGAAGGCTTGATCGCCCTGCATAATAGACGGAATAATCAGGCAGACGCTCTCCCCTTCAAGCTTGGCATAGCCGTTGCGAATACCCTTTTTGTAGATGATTTTCATGGGTTGTAAGAAATAAACTGAGGAAGGATTATTCTTTTGCTTGCTCTTAGTTCTGTTCAGCTTCTCTTCAGGATGAGTGGGGAAAAGGTCTCTGGATTCCCATGCTTCACTCGCAATGACAGATTTGAAGCTTCTGAATTGTCATGTTTCGCTCGCAATGACGGTTTTGAAGCCGCTGGATTGCTACGCTCCGCTCGCAATAACAGGTTTGAAGTCTCTGGATTCCCATGCTTCACTCGCAATGACAGATTTGAAGCTTCTGAATTGCCACGCTTTGCTCGTAATGACGGGTTTGAAGCTTCTGGATTGCCACGCTTTGCTCGTAATGACGGGTTTGAAGCTTCTGGATTGCCACGCTTCGCTCGCAATGACAGGTTTGAAGTCTCTGGATTCCCATGCTTCACTCGCAATGACAGGTTTGAGGTCTCTGGATTACTTCACTTCGTTCGCAATGACGGGTTTGAAGTCTCTAGATTGCTTCACTTTGTTCGCAATGACGGCGGGAGAGGATTAGTATTTGTAGGCACTCACCTCTCTATCCATTTGTTTTTTGATGTCCTTTTCCTTGAGGATCTGCTTTTTTTCTACTTTTTTGTAAAGCTTCCCTAGCCCGAGTTTGATTTTGATGAAACCACCTTTGGTAACTATGACTTCTAATGCAAGGAGCACCATCCCTGGCTTATCAAGCGCTGAAGCAATTCTCGTGATTTCTCTCTTGTTGAGAAGGAGCTTCCTCTTTGCTTTCGCTTGGTAATACGGAACAAGAATGGGAGAGGTCTTTTCATAGAGCGGAATATCCATATTATAGAGTCGGCATTCATGATTATCGATCCTCACTACTGCATCCTGGATATTGATATGCGAAGTCTTGATTGATTTCACTTCATGACCTTTGAGCACGATTCAGGCTTCATAGGTTTTTTCTATTTGATAATCGAAAGTGGCTCTTTTATTTCTCGTAATGAGCTGCATCTTGGCGCGAAAAAGCTAAAACAGCTTTATCATACGGAATTGATCGAGAAAATCAAAAAGAAATCTGACTCTGCTAGGGGAGAAAGTCCTTGCAATCGGGAGCGAAAATGATAGAGTTAGCTCGTTTACTTGTGGCGAAAGAAGGATGAAGATCATAGATGAACTAGAGCAATTTTATACTGCTGAGGCTCAGAAATACGCCCAGACGAGAAGAAAGCATCGACCTGATGGAGAACGCTTATTGGAAGCATTGGAAAGTTTAGAAATGAGGAGTCCGAAACTTCTCGAGCTTGGATGCGGCAGCGGAAGACTAGTTTCCTATCTTGACCACGCATGGGAAAGGAAGTTTGCCTATACAGGAGTTGATCTTTCCTCAGGGCTATTGAAGTATGCGAAGGAAGCCAATCCTGGACGTAAGTTTATCTGTAGCGAGATGACGAGTTTCCTTGAAGGATGTAAGCAGGAGTCGTTTGATGCAGTGGTAGCGTGTGCGAGCTTTCAGCATATCCCGTGAGCAAAAGCAAGATTGGCAGTGATGAAGAATGCCTATAGAGCACTCCGTTATGAGGGGCTACTCGTGATGACGAATCGAGCATTTTCAGAGTGGTTTCTGAGGAAGCATTGGAAGGTCGTGGTTCAGTCAGGATTAAAGGCTGTTTTTACCCTAGGAAATGCCGACTGGAGAGATGTAATGATCCCCTGGAAAGCGAAGAAGCAAGTATATCACAGGTATTATCATCTCTTTGGACTCGAAGAGCTGAAGAAGCTTGCGGAGATGGCGGGGTTTTGGGTAGAGAAGTGCTATTTTCTTGATAAGGGAGGCAAAGAGGTTGAAGACTGGCATAAAGCGAATAATTCGTTTTTGGTGGCAAGGAAGGTGATTTGTAAGGGGTAGATATAAAGAAAAGCAAAAAGATGCTCTCGGATTTGAGGGCATCTTTTTTAAATCTAGGCTGATTAGCGGCAAGTGTTTGGAACTCCATCATTACGATCTATAAACCTACAGGTAGTTTTTTCAGTACACCTCTGTTGGTACGTAGCATAAGTTCCTCCTCAAATAATAAGATTACTACAATAACGTGTTTGTGGTTGAGCTCCGACACATTTACTCTCTAGATGTTCGTTAGGACTTGATAGTTGATTCTTCCAAGAGCTACCACTAGGATCACACTCGGTATCATTTGTACACAGTCTTTCCTGTCCGAGATATCTTCTACTACCATTTGAGTGTATCGCATAAGTATCACACCTACCTGGAGTTATTTCTGTGTATGTTCATTCGCAAGTATAGGTTGATGGATGACCTCATCCACATGTTAATGGGTGACAACACTGTTGCGTATTAAAACATGATTCTCATTTCTTTATACAAGAAGGTCCGCTTGGACTTCCGGGACCACCTGGAACACCTGGAACACTCGGAACACTCGGAACACTCGGAGTACTCGGAGTGCTAGGAAGGCTGGGTCTCTCTTCCATCCCTCGTCCTGGGTGACAAGGACATTCTGCATATCTATAGATAATTTCTTGGCTAATAAATTGACCTGTAATTTTATTATATTTTCTTTTTGTGCAAGGAACCGGAACCCATTTACAGTAAATCCCTTCAGGACACATACCACAACCTATTTCTTCTCTTGTAGCATTACACCATGACATAGTATGGACACGAACTCTTCCTCCATTCGTTTGACTTCGATGTCTGCAGACTGAGGGATCCACTGTTCCATATCCGTCCGTGCAAGTATACCAAACGTTTTTTCGTGCTACACCTTCTGCTCATCCCAGATCACAATATGGTTGCCCCTCCTCTCTCTTCCACTGTGCTAGTATGCACTGTCCCCATCTCAGATTGAATCCTTCATCACATTCCCATTTACATGTTTTATTTGTAAAATTTCATCCCACGTAATGAGAATTCGCTGGTTTAGGGTCACATAGTTCACATCTCTCTTGTTGTCCTCCTGCAGATCGAGTCCCTTCATCCTTATTTGACTGACAGGGTCTACAACCTCTGTTATTCCCTCACTTATTATGATAAATATATCATGGCTTACAATTGAAATCACAATCCAACCTACTCGAAGATGGGTCTGTTCGGTTTTTCGTTCCGTTTGTGATGAATGTTGCGTTAGCTATTGGCGTACATGATGCACATTGTGCAACACCTCCTGATGAATAAGTTCCAGGCTGACAAAAAGTACAAGAGCCTGTAGTATTCGTATCATGTTTATATGCGAATCTTTCATCACAATGAAAATTACATGAATATTCACTATATCCCGTTGTCGTCCATGCTTTAAATCCTGAGTTGATCGTCGTTGCTGTCCCACCCCTTGTGCTTGCATGAGTGATAAATTCCTGCAACCCTTGTTTATTGATTGTTGGCATTACACAGTCTTTACATTCTGCTCGCTGGTTATTCTCTGGGGTATAGGTTCCTATATTACATCCACTACACGATCTTTCTACTCCTGGCTGGTCTTTGTGTTTATATCCTGCTTTACAGCCAAAATGACAGTTATCATTCCCTGTCCCTGGAGTCCACCATCCATTAAATGTTGAGCCGTCTGGTTTTTCTTTCGTATATCTATTTGGGATCTCCGTACTTGGTAAACATCCTCACATCTGCCATCCATCTGGGAAATCTACCCCTGCATGGTATCCTGTTTGACAGGTATATCCACACTCCTTACCCGTAGAACCTGGGTATTGGATTCCTCCATATACCCATTTTACCTCTTCCCAACCTCCGTCTCCTTCATTAAGCTCTGGATTCCATTTTGGTAACGCTTGAACTGGTGTTGTCTTTATTGTATTTTGCTCTCACCCTGCACATTGTCTTTGATTCATATTTACACAGACTCCGTGCGTAGATGGATCTGAACTGAGCGGAACTCGCTCTACTCCATTACATCCACAGAGACCGGATTTTCCTCATGCTTTTACATATGCCATCAAACCTGCATACTGCTCATCACATTTTCCACTCGCTGAAACAAAGCCTCATTTAGCAGCTATTTTTATTGCTCACTTTCCTCCGAGTCCTCCAATATCGAACTTCACCTTTGGATCGTTCGTATTAAATCAGATTCCATTCTTTGTATTTACGTAGAAGGCACCTGATTTCTTTGGGCTAAAGGTTCCGTCATCTGACCATACAAAAACTTTTTGGGCATTCCCTGCCTTGATATTTTTACCAATCAATACGCTATCAGAACCTGCTTCATTATTCCCTTCTCAGAGAATAACTACATTAGATCATCTTACAACATTCCCATGACCTCCAGCTACAAGGATATTTTCTTTTTTAGGTGCACTTATCCCTTCTGTTGGAACTCTCGTCTCAGAAGCACCAATAATCGCTAAGTTATTTGCACTAGCCGCTACAGTTCCCCTTCCTCCGAGGACAATAGAATTTGTGGCAGAAGCCAGAGAATTATCCTTTCCTCCCATAATCAGTGCATTTTCACTTCCATTCTGGATTTTATTCCCTTCTCCTCCAATTGCTGTAGTCCCTGAAAGCGCTTCATTCTTATTTCCAATAATCAGTGCATTAGCATTTACGTTTCCACTAATATGGAGAGCTTTTGCTCCAGTATCATAGTATAATGTTACTACTTTCGTATCATTAGGTCATTCATCCCCCATATGAAGTACCATTGAGGTAAGATATTGAGCTGAGTTTTCTACCCCCTCCACGGAAACTCTATTTGACTCAGAAATTGATGATGAATTTGCAGAGCTTAAATCTCAGAGCCAATTCATCCCTAAAAAAGTTCACACTCCTACCGCTCCAACAATAAGTGGAGCAGAGAGAGCTTTCCATCTAAGGTTTTTCTTTTTACTGGTAGTCATACTAGCACAGTTAAGGTGTAAAATATCTTTACGTTAAAGTATAAGCATTTATTTTTCTTTGTCAAATTTCTCTCTTTTTTTCCATCTTGCCTTCTTGCTTTTCTATGAGATTCGAATGAAAAGAGTTGCTCCGACAACATTCAGATTTTATACTGTTTCCTTCTTATTTTGAACAAGGAAAACTAAACTCCTACTTCTTCGTTAAGCGCAAAAAACGAAGATTAAGTTCACTTCAGGAGAAATAATGTAGGCGATTAAGGAATGAGTTTTTTGAGGAATTCAAAGAGCTCTGCTGTAGAATAGACTGTAAATCTTGCACAAAGTCCTTTAGTTTCTTTGTGGTATCTATTGATCCAAATTCCTTGCATTCAAAGCCATTGCGCTCCATCAATATCTTTCTGAATATTGTCTCCGACCATAATACACTCTTTGGTAAGCACCTCAAGTTTGTGGGCTGCAAGCAGGAAAGGAGAAGAATGAGGTTTATCCATTCCTGCCTCTTCTGAACTGACCATAATATCAATCTTATCATCAATTCCGAGATGAACAAGTTTTTTGAGCTGAGTATAATTGGAGGAATCCGTGACGATTCAGGTTTTTATCCCTTTTCTTTTGAGATATTTTAGAAGCTCTGTACTTGCTCTTTCTCTCTTGATCGTGCTATAAAAGACTTTCCAATAACGATCAAAAATATGAAGAATATCTTTTGGCAGGATTTTATTCTTCATTTGGCTGTTCATTTTTTCAAAAATTTTCTGAAAATACAGATCCTTATTGTGTGAAGCAGCTGTTCACAAAAGTTGCATTTTTACCTCTTTCTGTGCAATTTCAAATAAAATTGGGATAAGCTCTAAAGGAGTCTTGGTCAACTTATGGATTTCTTTAAAAATTTCTTTTGTCGCTTCTTGATTACAAGATTCATAGTCAAAAAGCGTATCATCAAGGTCAAACAGCACTGCCTTTATTGCCATATTTCCAAAACAAGAATAAATAAAAGATGAACCAATTATACTCTAAAAATCAGAATTTTACAACTTTTTTATAATCAGGCTTGCAAAGCTCTCCAATTTAGTTATTCTGAGAGTTAGGATTTATTTCTGCAATAAAAAACGTATGCCAAAAGCAAAAAAAGCTGATCTCAAAACTGACATTTTGAATAGTCCAATAGGAAACCAAAACTTCGCTCATTCTGAGGAAGAAATCGTTTTCCAAGGGATCAGAACCCACAACCTCAAAGATATTGACCTCATTTTGCCGAAAAATAAGCTCATTACCATCACTTGAGTGAGTGGATCGGGGAAATCTTCTCTGGCTTTTGAAACCATTTATAAAGAGGGACAATACAGGTATATTGAATCGCTTTCAAGCTATCTGAGGCAGTTTTTCAATCTTGGAGATAGACCAGAAATTGACTACTGTAGCGGACTTTCTCCTGCAATTGCGATTGAGCAAAACAAAAGAAGCGGAAATTCAAGGTCTACCGTAGGAACGCTTACCGAGATTGATGACTACATCAGGCTGCTGTTTGCAAAGCTCGGTGATACCTACTGTTATCACTGCGGTGCGAAGATTGAGCCAAAGACCATTGATCAGATCATGGATACGATCAAAACTGACTATCAAGGGCAGAAAATCTATCTTTTGAAAGAAAGCGGAAAGTTCTCAGAAAAGTCAGAATTATTGAAATTTGTCAAAAATAATAAACAAAAAGTAGAAAAAGGCTGAGGTTTTACGAGGTATCTCCTCCTTTCTCAGAATCCTGAAAAGCCAGATCCGATTGAATATTTTTACCTGGAAGAGCCGAATATTCCTGAGAGCTATTTTCCGCTTCAGAGCTATGGAATCTTTGATCGTTTGACCTTGGAAGAGCAAAAACTCGGCAGATTGAAAGAAGACATCATCAAAATCCTTGCTGAGACCAAGAAATTCTGAATCTATAGCACAGAAAGCAGTAAAGAGCTAGAAAATCAAACTTCAGAAAAGCAGGTTTCTTCAGACTTTTGAGCGAATATTGAAATTTCCACGAAAAAAAAGAAAAAAGCTGACTCCTCTACTCTGATGAGCGAAAGCAAGGGAAAGCCAGTAATTCAGCGGTTTACCGACAAAATGTATTGTCCAAACTGTAATATCACTTATCCTGAATTTACGACTCAGCATTTTTCTCCTAATAGGCAGGAAGGAGCTTGTCCGCATTGTTTGGGGCTGGGTGAGATTTTGCAGGTGGAATATGATAAAATCATTGATCCTCAAAGTCCGCTTTTGGAGGCGATTTTGCCATGGAGAGAAAGTAATCTTGGACAGTCTGTTTTAAAAAAGCTCTGTGAGAAATACAGCATGGATGGCGAGAAAAAACGAGCGGATCAACCAGAATGGTTTTTGAATGTGATTATTGAGGGAGATGATGAGCTTTTGAGAGTCAATAGCTGAGGAAAACGAACGAGCATCAAATATCACGGAATTGAAGAAATCATCAAAGATCAATATGCAAAAGGACTTTTGACCGTTGATTTTCAGGCGATGCTGAGTATGAAGAGCTGTCCGCATTGTTTCGGTTCTAGGCTTAAAAAGGAGAGTTTGCACTGCTTTTTGTGTTTGAATACAGATCGTCATTGCGAGGGAGGAACGACCGAAGCAGTCGAAAAAGCCACAAAAAAGCTGAAAAAAACCTATGAAACGGCTAAAAATCAAGACTCTGGATTGGTTGGCTTTGCTCGCAAAGAGGGAGTTGAAGCATTGATCCCATCACTCTTTATCAATCAAGAAGGTTTACCTTTTGCAAGACCAGCAGATGAACAGCTTTTGAAGGTAAATATCCGAGATTTGCAGAGAATGGAACTGAATCATCTGATCAGCTTTTTAGACCTTTTCAAACAAGTAAGTAATAAACCTTGAAATCTGCTTGATAGGATTCTTAATCCGCTTCTGGATAGGGCAAAAACGATTCAGGAGCTTGGACTCGGCTATCTGATGTTGAAAAGAGGGGTAGATACGATTTCTGGAGGGGAGATTCAGAGGTTAAGACTAGTAAAACAGCTCGGTAATAAACTAACCGGGATTATTTATGTGCTGGATGAGCCGACGATTGGGCTTGATACCTGAGAAATCAAGAAGGCGATCGCTTCTATTCAGAAACTCAAGGCGATGGGGAATACCATCATCGTCGTGGAGCATAATGAGGAATTTATCAAGGCTTCGGATTGGGTGGTAGAAATCGGTCCTGGAGCTGGAGATTTCGGTGGAGAGCTGATTTTTAACGGAAGCTATGAGGATTTTCTGCAAGCAAAAACGCTGACTTCCGACTACATTACTGGGGAAAAGAAAGTGAAGGTTGATTTTGAGCATAGTCCAAGCCACGACCGAGTGCAGATCAAGCACGCAAGTAAATATAATCTCAAGAATATTGATGTTAAGCTGAATCTCTGAAGTTTTACGGTGATCACTGGACCTTCTGGAGCCGGGAAAACGACCTTGATGTACACGACATTGTATAGATTTCTCAATGATAAGCAGAAATTCGTTCAGAGTTATATCAGACTTCAACTTTTGAAAAAAGGTTTGAGCTGGGAGGAAATCTTGGCTGCTCCCGTGATGAGAGCTGAGGAATACGCCCATTTTGAGAATCTCGCATTGCAAGAATTTTACCAAGATATCGGAGTAGAAAAAATCACCGGACACGAACAGATCAAAAACACGATCTATGTAGACCAGTCTAGCATTGGGAAAACTCCGAGAAGCTGCCCTGCGACTTTTGTGAGTGTCTTTGACAAAATCAGGCTCCTCTATGCAGGAACGACCGAAGCGAAGTATTTAGGCTTTACGAACTCGCATTTCAGCTTTAATTCTGAAAAAGGTGCCTGCCCTGCTTGTAATGGGTATGGCTACAAAAAGATTGAGCTGCAGTTTCTGCCTGATACCTATGTTCCTTGTGAGCTATGTCAAGGAAAGAGATATAAACCTGAAATTCTCGCGATCACTTGGAGAGGGAAGACAATTAGTCAGATTTTGGATATGTATATCTATGAAGCCTTGGACCTCTTTCATGAAATGGATCATATCAAACAGGAACTAGAACTGATGGTAGAAATCGGACTTGGCTATCTCAAGATGGGACAACCTGCACATACCCTTTCTGGTGGAGAATCACAGAGATTGAAGCTCGTGAAGCACCTTTTGAAAGAATACAGAGGGAATACGGTGTATTTCCTTGATGAGCCGACGGTTGGACTGCATCCTGCGGATATTGAAAGGCTTTTGAAAGTGCTGAAAAGATTTTTGGAGAAAGGAGATACGATCCTGATGATTGAGCATGATGAAGATATTTTGCAATTCGCAGATCATGTGATTAGGTTAGATAATGGGAAACTCGTAAAGTAAATTTTATCCACTAGGCAAGGCTATGGAAAAACTGACCTATACTACAGTTGACACTCCCTTTCAGTTTGAAATTCCAAAAATTAAGGGATCAAGGTTTATTACCTCAGTTTTCCCTATTGATAGTAAGGATCATGTTCCTGAGTATCTTTCTCAGATCCAAAAACAGTATTTCTGAGCAACTCATCACTGTTATGCCTGGAGAGCTGGCGTTCGTATCCAGGAAGATCTTTTTTGAGGATGGAACATCGTTGCTTTAGCTGAGAAGGTAAATGATGACTGAGAGCCTTCAAATACCGCAGGAAAACCGATACTGAACGTTCTGAAATGAGATCAGATTTTTGGAATTTTGGCTGTCGTGACGAGATATTTTTGAGGGACTTTACTCGGAGTCGGCGGACTGATTCAGGCTTACACTCTTGCCATAAAATCCACCCTTACTCAAGCTCCGCTTCTGCAAAAAGAAATTACAAAAAAGCTGATGATCCACTATCGTTATGAGGAAGTTTCCCGAGTTCAGCATCTTTTTGCTAAATATCAAGCGATCCCCTATTATGAGGAATATGGAGATAGCATTACACAAGGGGTTGAGGTAAATTATAGCTTTTTTCAGAACCTGACTAAGGATTTGACCTCATGAAGGATTGACTATGAAGAGGTGCTATAACTTAGTGGCAAAAAAATTATCAGTAAAAAAAACTCCCATACTAGGAGTCTTTTTTGTTATTTTTGAATAAGCGTATTGTTTTCTAATGCTCTGATGCGAGTTTTCTTTTACTTCTTTCCTCCTCAACCTGCTTCATAAGCTCCTCTACTCCCAGCACATATTGCTTCTTGCTCCTAAATTCTCTGACAGAAACCTCATTTGCGGATTCTTCTTTCTCTCCAACGATGAGAATATAAGGAACTTTCATCATTTCAGCATTACGGATTTTCTTGGAGAAACTATCGTTTCCCTCATCTACCTTCACTCTAAAGCCAGCTTTTTTGAGCTGTGAAGCCACTTGGAAAGCGTAATCATTAAACTTTTCTGCGACTGGCACGATCTGGAACTGAACCGGTGCAATCCATAGCGGAAACGCTCCTCCGAAATGCTCAATCATCACGCCCATAAACCTCTCCAAAGACCCAGAAATCGCTCTATGAATCACCACAGGTCTCTCCTTTTCTCCTTGTTCATTGGTAAAAGAAAGATCAAATCTGACTGGGAGATTGAAATCACACTGAATCGTCGCAAGTTGCCATTCTCTACCGATCGCATCTTTGAACATAAAATCCAGTTTTGGACCGTAGAAAGCCGCCTCCCCTACTCCGATCTTGTAAGGAAGCTGATATTTCTCAGATGCCTCTCTCAAAGCCCCCTCTGCCTGCTCCCAAACCGCATCATCTCCGAGATATTTCCCTTCTGGACCACGAATAGAAAGCCTTACTCGGTAATCATTGATCATTCCGAGGGTTGAATAGAATTTTTTGATGATTTCCACGATAGTTCCTACTTCCTCAGTAATTTGGCTCACACGGCAGAAAAGATGCCCATCATCTTGAGTAATAGATCTCACACGAGTCAAACCAGAAAGCTGACCAGTCTTTTCATCTCTATAGACGGTTGCAGGCTCGAAATAACGAACCGGCATATCACGATAGCTAAACTGATTATCAGCGAAAATCTGCATATGATGAGGACAGTTCATCGGCTTCATAAAGAAGTTTTCCTCCTTTCCCTTTACTCTAAAAAGCTCATCTCCAAACTTATCCGCATGCCCTGAAGTTTTGTAAAGGTCTTCTTTGGCAAGGTGAGGAGTCCATACTCTTTGATAGCCTTTATCTTTGTGAAGCTCCCAAAGGTAATCCTCAACTTCTTTTCTAATAATCATTCCATGCGGCTGCAAAAGTGGTAATCCTGATCCAACCAGCGGTGAAATGGTAAAAATTTTCAGCTTTTGACCCAAGATTCTGTGATCTCTTTTTTTGGCTTCTTCAAGAAGGGTTTGATATTTTTGAAGCTCTTCTTTGGTATCAAATGCTAAACCATAGACTCTAACCATCATCACATTATCAGAATTTCCTCTTCGGTACGCCCCTGCGAGTTTATCAAGTTTGAAAGCCTTGCTATCAAGCTCTTTGGTGTTTTCCACATGCGGACCTTCACACATATCAAGGAATGTAATGAACTTCCCAGCAAACTTTTCAGGGAACTTTTCCTGCAAATACGCAGTTACCTTTTCATAATACTCACGATAAGATGGGTCTATTCCCTTGAGTAAGGCATCTTTCGCACCCTCCACGATTGTATTGGCATAGAAGGTAATTTCCTCTCCTGCTGCCGCAAATTCGCTTCTCATCTCTTCTTTGTATTGCTGCTTCATCAACTCAGTAACGAGTTTTTTTGATTCCGCATCAGAAAGCGAAAAAAGAACGAAATCCTGATTTTCTTTCACGATTTTTTCCATCTGATTTTGGATCTTTTTCAAATCCTCCTCCTTGATCTGCTTTTCAGAGCTAAAGAGAAAGTCATAATAAAATCCGTTATCAATCGCAGGTCCGATCGCAACTTCCACATCTCGTTGCTGCTCTCTCTGCACCGCCTGTGCAAGGATATGCGAGAGCGAATGCCTTTTTTTGAGTAATGCTTGTTCCATTTTGCTGATTATAAAGAATAAAAACTACCTTCTTCCCTAAAGAAAAACTCCTGCAACCAGGCAAGAGTTTCTAGAGTATTGAACTACTACGAAAACCTTGCCGAATTAGTCGGTTGTCGTGCTCGTTGTAGTCGTAAGCTGTTGCATACTCAAAGGAAAGAACATAAATCTGACTTTAGTTTAGGATTTTTGATAGTTATTGCAAGAGAAAATAATAAAAAATCCTGACTACTCATTCAGTAATCAGGACTTTAAGGAATACTTTTGACTTACCATTGACTTTTTGCAAAGGTCACGCTGTTTTTTAACGGAAGATGAGGATTGTTCCTCCCTCTTCAGGAGTGATCATTGCAACTACTTCATGGGTAGGATTTACTTCCAATAAGGCACTAATAAGCGTTGCTAACTCTTCGCTACTCTTCCACCGGATATGTGCGACCTTTCCATATACTGCAAAACTTTTGACCAAATAATGATCTTTCGGAACTTCTAGAATTTCCATACTACTTTATTTTTACGTTGTTTGACTTTTGTTTTTTTGTATTATATCTACATAATATAAAATGTCAAGCTTAAATAAAAAAGAAGATCAGATTTTTTTGATTTATTTTTTTTTTGAGTTATACTTGAGTCAACATCATCACTTAAGACAAGGTTTCTACTACACACCCTTGCAAAATAGGTGACGATGTTGGTGGTATTCCACCTTAAAACGCTCTCCAGACCCACTACCCAATACGGTCGGACAGCATATTACTAGAGGTCTTGATATTTGTTTATATGCTCGGTAATAAGTAATTGAAATCTGAATTACTTGCTACCGATTGTATGAAATCGGGATCAGGACCTCTAGCAAGAGGCTCTGAATGGTATTTCCTTCAGAGTTTTTCTTTATTTAGAAAAATATCCCGTAGCAAAGACGGGATTTTCTTGATCAACATCTCCTATCTAATGAGTATGTTCTGCAAAATGTGCTAATGCATAGGTAATTAAGATAGCAACAACGAAAATCACAACCTGAACGAGAGAAAATTTCAGATTTTCGTTATGCTTATTAACCTCTGGTATTAAATCGGTTCCCGCTATATATACGAACATTCCAAGAGAAATTGGAAGCAGGATCATCTCAATATCATGTGCAAAATGCCCAATACTGAGCGTAATCACTACTCCAATAACTGCACTTAATGCCGTCAGAAAATTGAGGAGTAGTGCTTTCTTTTTTTCATAGCCTCCATGCACCAAAATAGCATAATCTCAGAGTTCTTGAGGAACTTCATGCATTAGGATGGCTAGAGATGTAGCAATTCCCGTCGGAATAGAAACGAGGAAGCCTGCACCAACAACCATACCATCCATTAAATTATGGATAAAATCTCCTAAAAGGCTCATTTTAGCGAAGGTATGTGGATGTGCTTTGTCTGGGCTATGATGACAATGATTCCAATGAAGAACTTTCTCAGTCAATAAGCCGATAAGCACTCCAAAGATAATCCAAAGTCCGATAATTTGTGGGTTTTCTCCTTCTCGATGCTCAATAATTTCTGGCAGAAAGTGAAAAAAGGCATTTCCGAGCATTGTTGCCGCGGCAAATGCCACGAGAGCAACGATAACCTTTTCTCATAAGCCTTTTTTTGCTCCAAGGAAAACGATCCCAAGGAGTGAACAAAACGAAATTATAACAGCACTTACTATTGAATAGATCCAGACTTCCATCTTTCCTTTTTTATAATGGATAAAAAACAGTATTTTACTTGTGTTGGTTTATCTTACAAAAGAGGAAAAGAAAATCAAGTCAAATGCTTTTCTAATAACATTTGATTAGATGAAGAATCCTAGCTCTCAGCTGGCATAGAATGTTTTACTAAAACTCAGTTAAGCAGTGCTATTCTTTGGAGCTTTACTCCCTGGCAAGAGGCTTACTCTATAACAAGGCTGCTCTAGCTCTAGAATGAGTCAATAGTAAAGAGATGAATAAGAAGCTGGTTATAAAGCCATTTTTTATCTGGAGTAAGTTCAGGGTTTACCATATCACGTATGATTTTTATGAAAGGGACTTGATATTTCTTTTTTCCATACTGCTTAAAGATACTATCTATAACTCATGCGACCTTTCTGAACTGAGCTTGATCTTCTGCACTAGGTTCATAGGGTTGAACTTTAGGGATCGCTTTGGGATCTGGGGTGATGCCGGTTAATTTGATGTATTGTTCTCTATCCAAAACCACGAAGGCGTCCATTACTCTTGTTCTCGCTCCTCTATCAAGTGTTTTCCCCTCATACACCATCGCAGTACTTGCTCCGGCATCGAGCGAAAGTGCATTCCAGCATCAGAATTCTTTTTTTAGATAGGCTGGAAGATTTCGGATGCTACTTGCACCAACAACTCCCATATAAATCGTAGTTCAATCTTCCGTAGAGCAGATAAAATTCCTATTTGCATTTCCTGCCATTTTTTTGTCAATATAATTGACGAATCCTTGCACAACATCATCTCCTTCAAGCAAGAGCACTGGGAAATTTCCAATTCCAAAATAAATATCATCTAATCTATCCTTGTTGAGATTACTTCGAAGTCCGACATCACTTTCTCATGAATTTCTCTGTACAAAAAATGGATTTCCCTGTTTATCAAAGGAAAATATCATTCTAATACTCGTATCTCCTCGGAATCTACTCCAATCTTTACCATTTCCAAGGAACACTCTCTCAGAAATAGTATGCGTCAGTCTTTTCCCGTCGATTTTACAAGAGGAATAATCATCCGGACAGAAGAATGCACCGTTAATTGCCGTATCTCCGCCAACGTTTTTTGCGAGCTGCTCGGTTGTTGCTCCTCCGTCCTTAGCGGGAGATGCTACGACAAAATGCTCCCCGTCCAATACGACTTTTATCACCCTAATCGGTCTGCCTCCTCTTTTTTCTATCAAGAGTTCCTTGTAAGCAAAGCTCATATTTACTAGACAAAAAAATCAGAATATTCAGCACATAATCAGCATTTTTATTGATTTCATTGATCTTAGCGAGAAAAAAATAAAAGTTCCTTATAGTAGGTTAGGTGAGTTTTTTTAGTATTCCTCGTTCTCTTTGGTATTTTCTTTGATTACTTTTTTGATATCGCTGAGTTTATAGCCTTTTCTCATTAGTTTTTGAATAATCTCAAAGCCGTCTGTTCCTCTTTTTTTATAATTTCTAATCTCTCTCTGAATACCTTGCAAAATTCCTTCCTGAATATCCTCTGCATACTTATCAGACAACTTATTAAGGAGCTGCTTATCGATTCCTTTTAATTCAAGTTTTTTCTTGATAATAAAAAGTGGTTTTCACTTTTTCATTACCTCGCTATTGAGATAACTTTCTGCAAAAAGGAGATCGTTTACAAAATTTTCAGACTTTAATTTTTCCAAGGTTCTCAAAACTATTTCTGAATCATATCCCTGCTGATAAAGCTTTATTCTCAGTTCCTTCTCTGTCTTTGGATATTTAGTAATAATCCCAATTGCATATTCATAACAGCTTTTTCCCATGATGAACGATAAAGATGGCAATAAAACAAAAGTCTGCTAATGGGCAGACCTTCTCTTATAATTATGAAAGGAAATTTTACAATTTTCCAAGCAAGTCGTCTAGTTTTTGCTCTTGGTCTGCCTTGACCTCCTGAGCCATTTTTTCTCTTCCCATCGTGTCCCTTACAGCATTTCGTTTTTTCAATCTCATTTCTCTCTCTGCATCGATTGCTCATTGTTCAGACTCTGCCTCTTCTTCTTTTTTTAAGATTTGGATATTCATCTGCTCTTCAATATAGATTTCGTAGAGTCTCAACATTTGGAAGGATCGAAGCTCAGAAATCGTCTTTACTACACTCTGATCTCAACTCTTTACTGCGATGTAGAGCTCATATGCGTCTTTATCAGTATTAAGCTTAAGTTCATAATCGCTAGAAAATGACCCGGCTACTAAACGTTGGAGCAACGCCCAATCAAAATCATCTCCCAATTCCTCAGGGGTCGAAAAGGGAATATACTCAAAATTGGTAAGGTACTCTTTGAATCGGATTTGGTCTTGGATTCCTCATTGGATCGGACCATATCGCTTTTCGTATTTTTCTACAATTTCCTTTTTGTCTGGCTTTTCTCTCATTTCATCAACAAAAGCCTGAAAGTTAAAAAGCATCGTATCCATGAATATAAAGATAAAAAATCTGATTCTCTCTAGTAAGATGTTTTTCTTATAGTAAATTTGTAAAGAAATGCAAGATGAAATTTTCCCTGCTTTTCTCTGCAAAAATTTTCAAATGAAAAGTAGTTATTCTAATTTCTCTTGGATATAAGCTTTTCGCTCTTCTCGAGGTGTTTTCTTCTCTTCTCATGGCTTCTCGGATATGGAGTCAGACTTTTCAGTCTGTTTTTTGAAGGCGATGATTAATTCTCAATCGCTAAGGATATTATTTTCGTGGGAAAGGAAAAATTTTGCATGATCAGAATTAAGGTATTTGAGCTGATAATTTTTGATATAGTCCATCTCCTCTTCGACACTAGCGGTCTGAGCTTTGATCGTGACGGTATTTTCGATGATAGTCGTATAGTTTACCAAAATTTGTGCAGCCATCAACATAAGAACGATATTTATAACCAATAGTCCTCGTTTTATGAGTGTTCCATAGCGTTTAACTTGATCTTTTATCCAGGGTATCATAAGATTTTTCTGCTATCACTAAAGTAAATCTCTTGATAATATTCTTAAAGTTTGTATAGTCTTTACTATTGTTTTTTGCTTTCAAAAAGCAAGATCAGATTTTTGTTTTTCTTTCTAAAGTTATGAGCATTCATAAGTTACCAGATTTTGTCATTAATAGGCTTAAAGCCTGAGAAGTTGTTCAAAGACCAGCTTCTTTACTCAAAGAATTGGTGGAAAATTCCCTTGATGCAGGAGCTACTGAGCTGATTATTACGATCAATGATGGTGGAAAATCCTTTCTTTCTCTCCAAGATAATGGTTGTGGAATTGAGCTTTCTGATATGGATCTCCTGCTTGAAAGGTATGCAACCTCTAAAATTGATTCAGAGCAAGACCTTTTTTCTTTGAAGAGTTATGGATTTCGTGGAGAAGCATTGGCAAGTATTGCGGAAGTCAGCAAAATCTCAGTTTTGACCAAGACAAAATATGCAGAAATCTGAACCAAACTTGTGCGTCGTGGAAATGAAAACGTGATCAAACATCTCGGAGTTCCTTTTGAACATGGGACTTTGATTACGGTTGAAGATTTGTTTTATAATGTCCCAGCAAGGCTGAAATTTCTCAAGTCAGCGCAAACCGAATTTTATTATTGCTACAATTATTTTGTAGATATTGCCCTTTATCACTATGATAAGGCCTTTATCTTGAAGAAGAATGATAAACTGGTCTTCGATCTCAAGCCAGCTCGTGATTTACAAAGCAGAATTTTGGATCTTTTCAAAAAAGACTGGTGAGAAAAACTCATTCCTCTTGCTCAAAAATTTGAAAAACTGGAAATTTCTGGAGTAGTTTCTGATGCATCGCTTCGTTTTGGTTCTGGTGAAAATATCAAAATTTATGTGAATGAGCGTCCCATCCAAGATAAAATCATTAGGAAAGCTCTCATGGATGCTTATGCAAGACAGATCACACCTGGGGAATATCCGATGGCAATTTTGATGTTCAAGATAGAACCAAGTGAGGTGGACGTGAATGTGCATCCTGCGAAATTACAGGTTAAATTTGCTGATTCTCAGATGATTTATCAAGTCGTCTATCAGACAATTTCTGAACTTTTGGGGAAACATAAGATTTGATTCGTCGGATCAGAGTTTTTTCAATGAAAAGGCACAGCTTCTCGTCCATCTTTTCCTCAATCTTCCGCGTTTCCGTCTCAGAAGCCAGATTTTTCAGCTTTTTTGACAGCGGAAGAAAAAGGGAGTGAAAAGTCAGATTTTTTCTCTCCATGAATTGGTGGAAGAAAAGTAGAAAATGAAACACTTTTTGGGCTATGAGGAAATCAAATTTCTCAGTTTGGATCGCTAGATGCGGAACAGCATCAGGAGCAGTTTTATTTTCACAAAGATATTGGGGAATATCAAATCTTAGGTCAGCTTTGGAATATGTACATTGCATTACAGGCAGATACAGCGCTTTACCTCATTGATCAGCATGCTTTAGCGGAGAGAATCGCTTTTGAAAAGATGAAAACTTCTCAAGATTTGACATCTGAAAGTTTACTTCAGCCTTTGAAATTTGAAGTAACGCAGATTGCTCATTTAGAGCAGAAAATCGAAGAACTTAATCAACTTGGCTTTGAAATTTCTCTTCTCTCTGAAAATGTAATTGTGATCTATGCGATTCCAAAGGTATTTGTCCAGTATCCAGTAGATATGTGAGTTTTACTAAATCACGTTTTGTATCTTGAAGAAATCACTTTTGATCATCTTTTGGATGGGGTCTATGCAACGAAAGCCTGCAAAGCATCGATTAAAGCTGGACATAAACTTTCCTATCTCCAGATGCAACAACTCGTTCAAGATGGATTTGAATTTATCCCTGGGATGTTCGTTTGTCAGCACGGAAGACCGTTTTTTGTGAAGATGGATAAGAAGCATATTGATGGGCTTTTTGATAGATAAGATGCTAACAATAGATTACTTCTGGCTCGAGATGGATTTCAAATTTTTTGAAAACTGCATCTTGCACTTTTTCTGCAAAAGCTTTGACCTCAGCTCAGGTTCCTCAAAGATTGATCAAAATTAATGCGTGTTGATCAGAGATTTTTATGTTTCCCTCTTGGTATCCTTTGAGTCCACAGAGTTCTATGAGCTGTCCAGCTGCGAGTTTCATCCTTTCGTCAGGCTGTAGAAAAGCTTTTAATTCAGGATGTTTTTTTTGAAGAGCCTCCCGCTGAGGAAGTCAGATTTCTGGGTTTTTAAAATAACTTCCAGCTGTTCAGACAACTCTCCAGTCCGGGAGCTTTCTCTCTCTGATTTTAGCAATCGTCTGAGTCAGGATTTTGAGCTTTTGAACGGGATCGAGTTCGTCAAAAGCAAGATGCTGTGCTGAGAACTCCTTCTGAATATCTCAGTACTCCACATTAAAATCATAGCCTTCTTTGATTTTTTTTAGGGAGAAGACACAATGCGTGATAATAAAGTTTTCTTTGAGTTCCTCTTTGAAGATGCTTTTGCGATAGCCAAATTTACATTCCTCATTCGTGAGTTTCTGAATGCTCTTAGTCTGCGTATTAACGCCGATGACCTCATATAATGCGTTTGATGCTTCTTGACCATAGGCTCAAACGTTAGAAACCGCACTTGTCCCAACTTTTCACGGGATGGAAATCAGATTTTCAATTCCTCACCATCTCTTTTCAATACTATAGCGAACGAAGTCATCTCGATCTTCGCCAGCTCCTACTTGAACAAGCACCTGATCATCAGTTTCATTCAGAATTTTAATCCCTTTGATAGCCATTTTCGCTACTACTCCGTCAAAAAAATCTGAAGTAAAAAGCGTATTGCTTCCTCATCAAAGAATCCAATGCTTTTCTGCGTTCCACTCAGCCGTTTCTAAGAGTTGGAGTAATTGATAGGTACTCTCTATTTCGACGAAGTATTTGGCTTTGCAGACGACTTGCAGCGTGTTATAAGGTTTGAGATCAATATTTTTCTTCATTACAGCGTAGATAAGGAGATAAAGCTATCAAGAAAGAGAGGTTTATTCTTGATTCTCAAAAATCAGCCTTCCCTCAATTTCTGCGGTAATTTCCTGGTGCGACTGAAAGTATTCTTCCAGAATATCAAAATCCTGCGTACAAACAAGTCTTGGAGATTTTCCTGATTTCAACTGGTTAAGATGGATGCCTAGATTTTGTTCGATATTATCAAAATGGAATTTTTGGATTCCCACGATATAGTTTTTATTGTCCTGAAGCGGCTGTCCTTGATAAAGGATGCTCTTTACTTCCTTGTTTTTCCAGTCATAAACAATAGAAACGCCCTTGCTCCATGCAAAGAACTCCTCCCCTCCTCCACAGAATCTGGATCTAAAAATAAAGTGAATCATCTCCTTGAGCTCCTCTCCAGTTAAGGTTAGTGCGTATATTCCTCCTCCGTAGGGATACATTTCTCTGAGTTCTCAAAGCGTAACGATACTTTCTAAGAGTTCATTTCTCAGCGATCCTGTCCCCAAACACATCATATCCAAACCAAGCTGTTCTCTGAAAATATCTGCAATGAGGTTACTCACTTGAGTTTCCTCAGTTCTTGTTGGATGTGTTAGGTCCTGAGCCATCTTGGATAGGATTCTATTGTACTTTTGATCAGTCTGATCCTTGTATCCTTTCAGAATTTTTTCTATTTTTGGATCTCTTGGACAGTAGGCATCTACAATAGGTATTGCCTGCCACGTATACGACTCAACACAATTATTGTCCGTATCTACTATAATATCAAATCTTCAAATCTGATTTGTCCCGACACCAACCTGAGTAACAAGGATATCCTTCACCTTTGTCGGCTGCTCTAGCAAAGTATGAGAATGCCCACCAATGATTACGTCGACTCCAAGCTCTGGATCAAGGAGTTCAGCGAGTTTGAGATCATTTTCATATCCAATATGCGTGAGAAGTATCGTGAAATCAATATCAATTCTTTTGTAGGCATTACAGATTCTCTCTACCTCTCTCGCTGCTTCCTCAACATTCACAAAGGTAGAAATCAGAGCATCAGAAGTCGTTTTACTCAAAGCATCTTCTGTAATGATTCCAATGAAAAGGATTTTCATCCCATCAATTTCCTTGATATAATAGGGCTTAAAAAGCCTTGTTTTCGTTGTTCTTATGTAAAGATTAGCGTTTATAATCGGGAATTTTGCAATTTTTTCTAGGAAGAGTAAGTGTGCTAATCCGTAGTCGATCTCGTGGTTTCCGAGGGTAACGACGTCTGGATTCAGCATATTCATGATATCAATTGTTGAAATCCCCTTAAACTCTGAATCCACGACAGATCCTTGGAGCATATCTCCAGAAATCGTATAAAGACAGTTTTTTTCCTCTTTTTTAACTTTCTGAATATAGCCAGAGAGCATAGAAACTCCTCACACGAGAGCCTGATTCTGCGTTTCCTCAAAAAAGTCCCCGTGCATATCGTTGGAATGCAAAATGGTCAATTTTTTGTAGTTTCCCATAATTGATGATAAAAAGATAAATCGAGAATTTCTGGAAAACGAAAAGCAAGCTTTTCGTCCAGACTTTTATTTCTTTTTTATGTAAAATATTGGCAAGAACTAAGAAACCTGATTGTATTTTTTGTTTAATCTTCGGGAAAATTTAGAAAGTCTAAGTATTCCCTAGAAACGATAATTTTCGATAGCTGAAATGATAAGACTATAAGTCGCATAATAAGGATGATCTTTAGCTACAGTCTTTTGTGCATTTTGGAAATTTGTTTTAAGAGCTGTAAGGAAAAGCTGCTTTTCAAGTGAGGTCATACTATTTCTTCTAAAGTAATCGTCAAAGAGAGCAGTAAATATCTTTTGTTTTTCTACGTCAGTATTAGAAGATTTAGTTTCAGCTGAAGATTGGTTTGTTGAAGATTGGGAAGTTGAAACCTGTTCTTGAGAAGTTGAGTTCTGCTTTGTCGTCTCTGAAGAAGTGCGTTGATTGGTATCAGAGAAAGAAGGTTGAGTTGTTTGTTTCTGAGGTTGAGAGTAGTTCTGAGTATTTTGCACTTCTCTAAACTTTTTGCGATAGATTAGTTTTCCTCTCAAGTATTTCCCTGATTCAAGAGTAATGGTCTGTTCAGGTCGATAGTGATCGAATTCGAAAGTGGTGAGCTCCTTCATAAAGCTTGAGAGATCTAGCGTCTCACCTCCCTTACCTTTGTATAGCGTACCTGCATAGATATTATGCCCCTCTACACTTTCATAATAGATATATACTCCTGCTTCTTTTCCCTGATTTTGTGAAGGAACAGAAGGTGTAGTCGAACTTGTTGGAGTTGTTGGCTTGTTTGGCTGGGTTGGAGTTGTTGGCTTGCTTGGCTGAGTTGGAGGCGTAGTAGGAGTCGTAGGAGTTGTTGGAGTTGTTGTTTTATTCTCCTGATTGTTGGATTCAAAGTCGTAATTACATGGGTAATCTGGATTTGGTATACAATTTGGGCTGTAATCTAGATAGGCAGACGTCTGAGAAAATCCGATCAAACTGAGCGTCCCTACTAGGGCAAGTGCTAACTTTTTCATGGGGAAATATAGGAAAAAGATAAAAGATATTGTGAACTATAGTAAAACTTTTAAAAAAAACAAGAGTAAATTTCCCCTTGCTTTTGGTCTGCAAAAGAGAAGAAAAAGAGCAAACTTTCGTCTGCTCTCTACCTCGATAAATTTTTAGTCTTTTATTTCAGGGTTTGTACCTTTCATCATTTCTGTAAACTCTTCACTCGAAAGATATTCTTTCTCTAAAAGGATTCCCGCCATTTGATGGATTAAAACGTCGTGTTCCTTCAGAATTTTTTTTGCTTTTTTGTAAGCATTGGCAAGATAAGTTTTTATCTTTTCATCTATAAGCTGTGCGGTTTGTTCACTATAAGGATTGATAAAAGGCTGGTCTTCTCCTTCTTTGAGATAGTTTATAGGTCCAAAATCCTCGTCCATACCGTATTTGAGAATCATATTCTTGATAATATCAGTCGCTCTCTCAAAATCATTAGATGCACCAGTAGTGATCTCATCTTTTCCAAAGAAGAGCTCCTCCGCTGCCCTTCCTCCGAGCAGAGTTACCACTTCATCAAGGAATTTCGCCTTGGAATAGAGGTTTTTATCCTCTTCTGGAGTCGTCCAGGTCACTCCTAAAGCATGCCCTCTACGAACAATCGAGATTTTTTCAATCGGATCTGCGTTTTCCAAGAGATGTCCTGTCACTGCATGTCCAAGCTCGTGGTAAGCAACCAGCTTTTTCTCTTTTTCTTTCATAGATTTTACTTTTTTCTCAGGTCCCATAATGACTTTCTCGAGTGCGTATTCAAAATCTTTCGCTTCTAGCACTTCTCTATTGGACTTTGCAACCTGTAAAGCCGCCTCATTCACGACATTTTCAATATCTGCACCGACTAAACCGCTCGTTCTCTTCGCAAGCGATTCAAGATTAAGCTTCTTATCTACTTTCTTTGATTTCAAATAGTATTCAAAAATCTGAATTCTTTCTTCTAATGTTGGCACGGAAACAAAGACTTTTCTATCAAACCTTCCTGCTCTGAGTAAGGCAGGATCCAAGGTATCAGGCCTATTTGTAGCTGCAATAACAATAATATTTGTATTTTTATCAAAACCATCCATCTCGGTGAGAATTTGGTTGAGGGTTTGTTCCTGCTCCTGATGTCCTCCGGTATAGCCACTTCCTCTCTTTTTCCCGATTGCATCAATTTCATCAATAAAAATAATCGCTTTTCCAGCAGCTTTGGCCTGTTCAAAGAGTTTTCTCACCTTGGCAGCCCCCATACCTACTAACATCTCCATAAATTCAGACCCAGAAGCCGAGAAAAAAGCGACTCCAGCCTCCCCTGCTACCGCTCTGGCAAGCAAGGTCTTTCCTGATCCTGGAGCTCCATAGAGCAATACTCCCTTTGGTGGTCTGGCACCGATTTTTTGGTATTTTTCAGGCTCTTTGAGATAATCTACGATTTCAATCAGTTCGTTTTTCACCTCGTCCATCCCTGCAATGTCAGTAAATCTGGTTTTAGATTCAGATTTTTTGCTTTCTTTACCTACTTTAATTCATAAGACGCTTCCTGGACCGCCCTTTGGCATAATAAACCTCATTCCAAGCACGAGGATAAGAAGGAAAAAAATCAGATAGCCTACCTGTTCAAGCAATTTATTCCAAAGGGAAGGCTCCTCATGCACGATAGCAACCTCAATATCTCCAGTCAAAGCTACTCCAAGATCAATAAGATTGAGCGAAGCTGACTTTTCTGTTTGATAAGCCCTATAGACTTCTGTTTCATACTCTCTACCAAAGAGCTGGCTTTCTTTTGCTTTTTTGAGAAGAGAAAACCCTTTTAGATCCTTGTCATTTGTCAGGGAAATTTTTTGATAAGCACCGTCATGATAGTTTGCAAGCCACTGGTTGAGTGAGACTTTTTCTGTATCTTCTATAACCTGAATAACTCCAGTATCCACACCAAATGCAGGTATCAGACTATTATTTTGGACTAGCCTCAGCCCAATAGACACTAGAATTATGAAAAGAATCCCATAAATTCGCCACTTATAGCCTATAATTGATTTTTTTTCTTTTACCATATCTTAAGTATGCGTTGAATAAAAACACATCCTTATAGTAAAGTTGGAAAGGATTGCAAGAGGAACGGAGAAAATGAAAGGAAGCCATTATCAACTTCATCCCCACCAAACGAAGAATCTCTTGACAATTTTGGAAATAATACTATACTGAGTCAGACTTTTTATCCCTTTACCAAAGGACTGAGTATTTGGGCTACTCCTCCATTGCCACTTCATCATCGTAACAAAAGCCACGCATTAAGCGAACACTTACAAAAATTTCATACTTGAATAACAAAAAAGCAAGGGCAAAGACTTATGAAGGAAGCAATCCCATTTATTCATTATCTCTATACTGTAGAAATGGAGAGACAGATTCAGTTTTTTAAAGTGAGCTACGCTTGATAGTAAGACTTTTTTGGCTAACTTCAAAGACTTTCCCTACAAAAAAGTTTATAAAACTTGACAAATTTCAAAATAATAAATACTATTAGTTATGTTTTCTTTATTTTCTTATACAATAACTGATGAACACACCTAAAAGTAACCTTGAACAGAAAAGTACAATCAATAAAATCCAGAGACTAGCTATGGCTTCAGCTCTTGTTATGGCTCTTCTTGCTGGTGCACCTGGATGTAAACAGTGAGCGAAAAATGAAAAACAAACCCCTCAGACAGAACAAACAACAGAGACTAAAGACCTTATTGCTCACGCTGCGGGGAACTCTTCTGCTAATACTAAAGATCCCTCTGCATGAATGGATGCCTCCAATCCTGCGGACATGATGAACAATTCAGCAGAAGGTGAAGAATGGTCAGAAGAAGACTTCGGAAAACTCATGGAAGTTGTTGATGTGGAAATTGAAAATGAATACTTAGAAGAAGAGAATAAGAAGCTTGATGAACAGAATAAGAAGCTTGATGAACAGAATAAGAAGCTTGATGAACAGAATAGAAAAGACCGTGCAGAAATATTAAAACGTAAACACGAAATAGAAAAATGGATCAATAGTCTTTCTGTAGAAGAACTACTTGCAGTCGATGGCATAGAAAAGGCTACTCATGCTTATATGGAAATTTGCAAAGGGTTGAACGAAACTCCAAACCCTAAAATGGTAACCGTTCAAAGAAAACTTCTTGCGAATCGTTAGGAAACCTTACTTTCATTCAAAAATAAATAATGGCTATATTTGACGAAAAGGTCTCAGAATATAAAAAACCACTAATTAAACCCCATCAAGAAACAACATCCTCCATTTCAAAGTTTGTTGAAGAAAAGCTTGGGAAGGTTTCTCCTGCTGAGAAAAAAAGCATTTATGATCTCATCGAAAAGAAGTTAAAGAATAAGATTTCTCCAAAAAATATTATCGTAGTTCTCCAGCAATCTGAAGCCATTGCTAAGAGGAAAAATATCCCCTTTTTAGAGGTCTTACAAAAAGTCATTAGGCAAGTTGAGCAAAATTTAGCCCAGAAAAATGATATTAAAAACTGACAAAAATTAATTTCTGTTCAAACAAGGAATAATTTGAATAAGGGAGCTACGAGATCAAAGATTCAAGCTCAAGAATCAAAGATTCAAGCTCAAGAATCAAAAATAAAATTTCAAAACTCAAGTAAGGAAATTAAAAAGAGTTTGAAAGTTTTTGAACAAACAAAAGAAGCTTATCAAAAGCTTAATCCGAGTCTCAAGGAACATCATCCTGAAGAAGTCCAGCAGAAGCTAAAAGCCCTCTCTCCTGTCGTAAGGGCAAAGCTTAAGAAAAACAATATAGCTCCTGCTGAATATGCAAACTTCCTTCTCTCAAGAGAAAAGATTAGATGAGATACAAAAAAACCTGAGAATGAAACATTCCTTAAAAGCTTAAAAAATATGGAAAGGTCGCTCGGAATCGAGGAGCAGACCCAGTGAGGTTATCCCCTTTCCTATGAGCCCAAAGCTAAGGTCTTTGATCAGAATCCTGATCTCGCTGAGTTCGCAAAGAATGATCAGACTTTTTCTCGTTTAGAAAATGTAGAAATATTCCCTAAAGGAGATGAGTGGGATCAAAAGCTCATTGAGAAGTTTGGAGATCGTGGACTTAAAGAGCTTGCTGGGAAAGTTGCCTCTTTGGATGCAAAACAAAAGCAAGGAGAAGCACTCTCCATACAAGAAAAGGAAATGATAAAAATATATAATTGATTAGCTGGAATACAAAAATTAATCACTATATCAAAATTAAATTTTTCTAATAAAGTCACAAACTTATATTAATAACAAATACAAATATACTTGACAAATTTAAAAATATATGTATCATTTAGGTGTGTATTTTGTTTTTTATATTTTTTATAGGAATAGCAATGAAGAAATCTGTAAATCATTCTGTAGAAACAAAGAATGTCAATCCATCAAACCTACAAGATAAGCTAAGGAAGCTTACAACCAAGATCGCTCTTGCAACTGCTCTCTTGGTAGGATATAACTCTTGTGGGAAAAAACAACCTACATTGGAAGACAAACAAGAAGTAAAGATGGAAGTCATGGATACAAAACAAAAGAATGAAACTGGCAAGAACGAAAGTAATGCAATGGACACAGAAGTTAAAAATGTGGCTAATGATACTAAGAATGATCAACCTGAAGAGTTTAGCGATGAATGGTATGAAGCATTTGCTCTGTCGATAGCAGTTGATGAAGAATCTCAAAGACTCGATGAAGAATCCAAACAAATAGCTGAAGATAGGAAGAAAATAGCTGAAGATAGGAAGAAAATAGCTGAAGATACTGAAAATGCTATGCTTTCTATGGAAAGGAACCGAGAGTGAGTAGACGAGGATCTTCTTGCATTTTCTAGTGATTTGCAAACATCTTTGGATGAATATTTTGCTGCCTGTAGAGAGCTAGGAAGAGAACCTAGCGCTCACTGTAAAAAGTTGATGGCGTCCCGTAAAAAATAAAGATGAATAAAGAGAAACTCAGCATACATCCTGATACATCTGGAGAGATACAAAAAAAATCACTGAATCCTAATGAGGTAAAAGGCATTATTGACGTCGTTTTTGCTAACCTTTCTCCAGAAGAGAAGTCTTCTATAGGGAATGTAATTCTTGATTCTAAGCTCAAACTTTCTCTTAATAATATAAAGTTGATCCTTGAACAGGCAAAAAAAGGATCGGAAAAAACAAAAACCTCTCCATTAAATTTTGTGAGGAAGTTTTTGGACTTTCAAAAAAAACAAATGAAACAAAAACTTGATATACAAAAATTCCCTACTTTATCTGCTAAAGCACAGGACAATAAAAAAGAGAACCAAGTCAAAGAAAAAGAGAACCAAGTCAAAGAAAAAGAGAACCAAGTGAAACGAAAAAAAATTAATATAAATGAGCGTCTAACCCAGCAAATAAAGCAAACAAAAGAAGCTTATCAAAAACTTAATCCTACGCTTAGACAACATACCTCCGATGAACTTAACTCAAAAGTCTCTACTCTTTCTCCTGTCGTGAGGGCAAAACTTAAGAAAAACAATATCGCTCCTGCTGAATATGCAAACTTCCTTCTCTCAAGAGAAAAGATTAGATGAGATACAAAAAAACCTGAGAATGAAACATTCCTTAAAAGCTTAAAAAATATGGAAAGGTCGCTCGGAATCGAGGAGCAGACCCAGTGAGGTTATCCCCTTTCCTATGAGCCCAAAGCTAAGGTCTTTGATCAGAATCCTGATCTCGCTGAGTTCGCAAAGAATGATCAGACTTTTTCTCGTTTAGAAAATGTAGAAATATTCCCTAAAGGAGATGAGTGGGATCAAAAGCTCATTGAGAAGTTTGGAGATCGTGGGCTCAAGGAGCTTGCTGGGAAAGTTGCCTCTTTGGATGCAAAGCAAAAGCAAGGGGAAGCACTCTCCATACAAGAAAAGGAGATGATAACCGCATATCAGCAGCAACTTGAAGCACTCAAGGCACAGATGCAATCGAGGCTGACCAATTATCTCAAAGCTTCTATTACTCAAGCTCCGGTAGTTGCGCTGATGCGTTATCTTGGGCAAGAAAGTCTTGGAGGGAGGAAGCTCTCTGATCTCCTCTCTCATCAAGACAAACCTTTTGCTACGCTTGTCCAAGAGACGACGCTCACGGGAAAGGCTGAACCCATTCTCCAGCTCAAAGGAATGATTGAAGGGAAGCCGTTGACCTTTTATTACAATCTTGCTGATCCAACCGCTAGCTTGGAATGTGACGACAATCTTCACTATGATCCCTTTTCTCATCAGCTTTCTTTGTGAGTTCAGGGGAAAGGGAATAGGACAAAGCTCAACCTTCAGATGCCAACGACCAAAGAGCTTTCTCAGCGTCTTGAAGAAGCATTGACACCAGAGAAGGTAAAAGATATTATTGATCAGGCTTCGGATCTTCAAGACTATCAGGAGAAAATCTGACTTTTAGCGAGCGATCAGCTAGAAGACTCCTTTTCTACGGATGAAACGATCCATTCACGTCTTGCCCGTCATACTGAGAAAAATCTTGCCATTCAGGAGCTTCAGGCACAATTGGTGCCTCCTGAGATCACGCACCAGCTGATGAAAGACGGCTTGATTAACCAGCACCGTCCTACGCAAAACGTATTTAGGCTTTTGGATCGCACGAGCGAAGAGAGCACAGACGCGGAACTCCAATCTTTTAGAATCGCGATAAAAAAGCTGGATTCCTTCCTCAAGTCTCCAAATCTCTCTCAAAAAATTATGGAAATTCAAGATCCTATCCTTCGTAATGTCCTCACAAAACTCTCTGATGCAAATCTAAAGAAATCTGACTTTCAAGTTCGATCGACTGCAATGCTAGATTTTTTTAACCTTTTCACCAGGCAGAATCTGACAGACAATAGCGTAAATCCTGAAGCTCCAGACTACAAGATGAATCTTTCAGACTTTTCTCGTTTTATTGGTTTTTTGGATCGTCCTGATCAGATGATTACCGATCCGAAACATTTGGAACATTTTTCACCAGCATTTCGTGAGAAGTATCAGCTCCAAGAGTCCGCTCAGCAGGGGGGGCTTTCCCACTTGGAGGATGAGATTGCAGCGGTCTATGAAGAGGAGCCATCCTACGCCTATGCATAGAAGAAGATACTTGAATCGATCGAAAACTTAACTAGATTGGTTCGCTTCCTTCGTAATGACTGGTTTACTTTGTATTTTTTGAGAAGAAAAAGAGGAAGGCTGATTCCCCTCCTCTTTTGTCTGATTTTTAGAACTATTTTACTTTATAAGTCCCAAGGATAGTTACCATCTCTTGATACTGGGCTTTGAGCGTAGCGTTTACTTCTTTCATAAGATTAGTAAAGGTCGTCTTGAGCATCTCGATAACAGCAGTTTTTTCCTTTGCAGTTGGGAATTTATCGGCGTGCTTTGCAAAGTGATCATCAAGGATCTTATGAATCTTTTGCTCCTGAGTAGTTGCTTTAGCAGGACATTCTCCTCTGTAGTATGCCCATTCATCACATTTTGTACCATCAGAAAGCGTACATACTCCAGATTCATTACCGTTTTTGTCTTTTACGATCTCATGTTTTCCTCCATTCTTTTCACAGTTTACAGAAGCAGGATTAGCCATTCCTACTGTATGTGTCGTCTTTTTTGACCAAAGGTATTTTGACCCGCTTGCTGTGGTAAGCTCGAGTTGATTTCCTGTCATAAGCTGATATTTAGCATTAGAAAGGTCAAATTTGCTCTCAGCATCCATAAGAGGCGTATTGCAGTACATGAGCGTAGTAGCCATTGGTCCTGCTAGGATCTGGTCTCCTGAAAGGGAATAGGTCGTAGAAAGATTATTGCAGAAAGATCCGAATACCTTTCCTGTCCCAAACTTTACTGTTGCTCCCGTCAAAGCTTTCCCTTCAAAAGAAGTGAGTTCCCATTCGGTTGCCTCGAGAGGATTGATTTCTGCAGCGAAGATTCCGCTGACTGCGAGTGATGCAGCACAAAGCATTCCTGCTGCCATAAGTGTTTTTTTCATAGTTTTTTTGCATAGAAAAATAAAAACGTTTTCTATACTGGATAACGCATAGGATTAAAGAAATGTGACAAAAAAAATCTGACTTTTGTCGCTGGTGGGAGATTTTTTGGTATGAAAAGTGCTTGTAATTTGAGAGGGTTTGACTACATATAAGGGTGATTTTTTTATTTTATTATTGATTAGAGATGAAAATTCTTGTGCTGAATAGTGGTAGCTCTTCATTGAAATACCAGCTTTTTAATATGGAGAATCAAGAGGTTCTGGCTAAGGGACTTGTCGAGAGGATTGGGATTGACGGAGGGAAAATTAAGCATAAAACCGCAACTGGAGTAAAGCATGACATCGAATGACATCTTGCAAATCATGAAGTTGCTTTGCACAAGGTACTTGAACTTTTGGTTGATCCTGAGCTTGGAGCTTTGGGAAGCCTGTCTGAGATCAATGCGATCGGACATAGAATGGTTCATGGAGGTGAAAGCTTTAAGCAATCAACGATTATTACTCCAGGAGTAAAGGAAGAATTTAGGAGATTAACAGCACTTGCTCCGCTTCATAATCCGGCTAACCTGATGGGAGTAGAAGCTTGTGAAAAGATTCTTCCTGGTGTGCCTAATGTTGCGGTCTTTGATACCGCGTTTCATCAGACTATGAAACCTGAGCATTATCTCTATGCACTTCCTTATAGATGGTATCAAGACTATAAAGTGAGAAGATATGGTTTTCACGGGACTTCTCATAAGTATGTAAGTCAAAGATTAGCTGAACTGATGGGAAGATCAGATTTAAAGGTTATTACCTGTCACGTGGGAAATTGAGCGAGCATTACTGCTATCAGGAACGGAGAAGTTGTTGAGACTTCTATGGGACTCACTCCTCTTGAAGGGGTGATCATGGGAACGAGGTGTGGAAATATTGATCCAGCTATTGTGCCTTTTATGATGAAGCAAGAAAATCTGAGCGTTGAACAGATTGATACGATAATGAATAAGGAATCTGGGATTCTCGGACTCTTTGGTAAGTCAAGCGATCATAGAGACGTTGAAGATGGCTACCTTGCAGGCAGCGAAAAAGAAACCATGATCTTGGAAATGTATACGAATGCGATTTTGAAATATATTGGTAGTTATGCCGCACTACTTGAGGGCGTTGATGCTATCGTATTTACAGCTGGAGTGCTAGAAAACTCTATCATTCAGAGAAAGCTTCTTGCTGAAAGGCTTGCTTGGCTTGGAGTGGATTTTGACGAAAGCTTAAATAACTTTAGAGGAGAAGAGAAAAAACTGACTAAAGAAGGTAGTAAGGTTCAACTTTGGGTGATTCCAACTGATGAAGAACTTATGATTGCAAAGGATACTTATGCTCTTACTAAATAGAAAATAAAGCCCTTTCTTTCAAAGCATTGTGAAGATTTTAGCTTATAAGGAATACTAGCTGTAGAACGCTTATCCTGCTTTTGTTGGTAGATTGCTCTATAGTTGTAGTGTTCCTTTTATATCTTTAGAAAAGATGAAAAAAATCTGACTTCTTATTTTGTGCATCCTTTGCCTAATGGGAATCTCGTTTGCGCACGAGATTGATCCGATGCATTTGCCGAGATTTGAGTATTTTGTGAATGATTATTCTCAAGTCCTCAGCCAAGGGGAATTTGAAGAGCTCAATCAACTTGCTGAGACTCTTGAAACGACGTCAGGATATCAGGTCGTAACGGTTCTTTTTCCTCATAGAGAAGGGAATGAGCTTTTTGATATTGCACTCAAAGCGTTTAATGAAAACGGAATTGGAGATAAAGACAGGAATGATGGACTTTTGCTAGCGATTGCAACTGAGGAAAAGAAGATCAGAATTATGGTTTGATATGGATTGGAAGCTGAGATTCCTGATGCTCTCGCCAAAGCGATCATTGAAGCACTTATTAGACCTGAAGTAAATAGCGGAAACCTCTATCAAGGAATCAAGAATTTTTATAGTTTTTTTTCTGATCAAGAGATAAAAGCTCAGGTTAAGCAACTGCTTCCTGAGGAAGAAGATTCCAGCTCTCAGATGACCTTTCTCTGAGTTTATGCGATTCTTTTGGCTGCGTTAGTCCTTCGAGGTTCGACAGGGAAAGGTGGATCTTCTAGAGGTAGTTGGAGAAGCTCAAGTTCAAGTTCAAGTAGCTCTTCTAGCTGGGGAGGAAGTTCTGGAGGTGGAGGCTGATTCTCTGGAGGTGGAGGAAGTTCTGGAGGCTGAGGTGCTGGAGATTAACTTGCAGTTTGAGAGCAAAAAAGTATAAGAAATCAGACTTTTTATCTTTTTTTTGAGAGAATAGATGAAGAAAGGAACGCTTTGGGGAATCGTTATTGCTGTGATCTTGATCGGAATTTCTGTGATTGCAGGTCAGTATAATTCCCTTGTAAAACTTGAGGAAACCGTCAAAGCCCAACGAGGACAGGTAGAATCCGTCTATCAAAGGAGAGCCGATCTCATCCCTAATATCGTAGCTACTGTCAAGGGAGAAGCTGCTTTTGAGCAGGAGACGTTGAGACAAGTAGTAGAGGCGAGAGCTTCTGCGACACAGATGAAAATAGATGCAAATAATGCACAAGACTTTGTAAAATTCCAGGCAGCTCAGGGAGAACTCTCTCAGGCATTGGGAAGATTGATGATGCTTACTGAGAACTATCCTAGCTTAAAAGCCAACCAAGCCTTTAGCGATCTCAGAGTTCAGCTCGAAGGAAGTGAAAATAGAATTGCTGTAGAAAGGATGAACTACAATAAGGTTGTAGGAAAGTATAATATCAAGCTGAGAAGCTTCCCGACCAATCTTATTGCTAAGTGGCTCGGATTTGAGAGAGCAAACCTCTTTGAAGCAACAACGGGTAGCGAAGTTGCGCCTGTAGTAGCATTCTAGCCGAGGACTCGAAGAAAAAAGAAAGAAGTCTGATTTTTGACTTCTTTTTTTAATTATTTATTTAAGATTTCTTCAAAAAATAACTTGACTTTTGCCTCCTTTTCTCTATCTTTTTTTACCTAGCTTTATCCACTTGAGATAAGTTTGCTTGTGAAAGTATGCTTATCTGGAAACGGGCGTAGGTTTCTCTACCCCGTTTTTTTCGTATTTGCAGGAATATCCCTCTTCAGAACTGGAGAATATTTCTTTAAATAATGTCTTGACATTTTATAGAGATTACGTATATTTGTGGTTGCAATTTTTTTTTCATCGCTAGAATTCAAGATGGAGACTATTGTTTGTGAAAACAAGAGTTTCCGAGAGCAGGTAGAGAATCTCTGCCTGCTTTTTCGTTTTTTACAAAAAAAGACAGCCTGGAGGGACTGTCCTCATATATTTAAGGATTTCACACAAAGACATCATGAAGTCTTAGAGAAGAACGATATTTTCTTTTAGCTCTTGGATTGACGATCTGATTTCTTCAAATCAGAATGCAAAATTACTTCTCAGATTTCCATTTTCTCTAAGGAGCATTGTCAGCTTGTCCTGAATATCTTGCATCCCTTCGACAAGTAAAAGGAGATCTTTAGTGGATTGAATCAACCCATTATCTAGCTCTTTCGCTAATCTGGCGATGTGAGCTTTCAACTGGAGAAGCGTGCTTACTACTACATCTACATACTCACTTTCAAAATGATGAAGGATTCCCCCTCCGTATTGACATGCCGCCTCTGTAGTGTATTCTAATGCAGAAATCAGAGCATCACAAGTTGTTGCAATTTTTTCGAGAAGTGTATTCGTTTTGGTAAGCGAATTAGAAAATTTCATTGATTCGTTCATTGGGATTAAGACAAGCTAAAAATGATTACCTCTCTATTATATTCCAAAACTAAAAAAAAGAAAAAAAATCTGATTTTTTCTCTTTCTTGATACTTGCCTTTTTTTGAAATGATGATTTAGTGCGATTTGTCTTGCGAAGAAAGGTAGGTAGCACAAGCAAAACTAGATGTCCAACACCGTTGAAGATTGAAGCCTCAGGTCTCCCCAGTGATATTAAGCGACTCTCCAATAACGAATACTCAAGGAAGCTTTTTGAGCTCGAGATGCTGGTCCACCTCTTCAAAGAGGATCCCCCCAGACATTACTTTCGCTTTTTCTCGAGAGCCTAGGCGAATTGGAGTCAAAGTCATCGTATATTTTCTGAAGCCGGTTTTTACCCTTAGATAGCTAAAGAGCCTCTTGGTCATAGCACTTTCTGGGATTATTAGTCTGATTTTTAGATTTTTTAGCACGTCTCCATACTGATAGCCAAGTCGAAGTGAAGTATTAAAAACAGCAGGTCAAGAGGTTCCTTCATGCGTAAAGAGCAGATCTCCTTGCGTTTCATAGACACATTTTCATCAGTCAAGAAGCTGAATTTTTGCAAACGTGCTACTTCCGGAAAGTTGGTCGATCTTTTCCTGCGTTTGGATTCCACAGAGGGCAGGATGAGCAGCTTTTGTGGCGATTTTGTACTCCTTTGCTAGAGTAAAGGCAAACTTTCCTCCTCCGATCTGCGGAAATGAACAGCCTCCACTAGCCAGAATTATCCTTTTAGTGGTGAAAGTTTCATTTGATGTTTTGACGGAAAAACCTTCTGCTATTCTCTCTACCTGGAGGACTTCCGTCTTGGTGAGGATTGTTGCAGCATTTTTCTCATTCTCTTTGATCAGAAAATCCACGAGTTGACGAGATTTATTGCTCTCTAGGAGGATTCTTCCAGCGTCTTCCTCTTTGCTTTCGATTCCGTGGCCTCTAAGAAAGTTTATCATTTCTTGCGGTCAAAAAGCTTGAAAAAAAACAGAGAGTCTCTCTCTTTGATCTCCAAGATAATGATCTGAGCTAGTATTAAGATTTGTGAAATTACACCTCCCCTTTCCAGAAAGCAAGAGCTTAGTTGCTATACTGTCTGTCTTTTCTAGGATCAGCTTTTTTCGCTGTTTTGGCAATAGCATTCAGCAAAATAAGCCACTTGCCCCAGCCCCAATAATAATCACATCTTGCATTTCTCTCGGAACTAAAGACTAAAAAAGACCTGTTCAACTGCCCTGATTATTGCATCACCAAAATAGAAAAAATCAGATACGAAAATAGGAAACACCCACTTCCGAGCAGTGCTCCTATAACGACATCAGTAAATCGATGAACATGGAGATATCGCCTACTCCAAGCAACAATTCCCCCGCAGATGATCGCAAAGACAAAGATTACTTCACGATATTCAAGGTTGAGGAGCCGAGAAACAGCAATTGCTGCACTCAAGAAAAATACGAATCCTGCTGTGGTATGTCCGCTTGGGAAACTATAGTCTTTGAAGGTGATTTTTGCTTTTTCAGGTCTTGGTCTTTGAGTTATTCTTTTTACGCTTCGGAAGGCAATTGATGCGACCAAAATACTACTTACTCCGATTCCAAAAATTCTCAATTCTTTGCTCCAGATGAGGATTCCGCTCATTAGAAGATAGAATATTCCGACTGATAAGGGAGTAATTGCATGATTAATGAAAAGAGAAAGCTGAAGTTTCCATGAAGTATCGTTTTTTTTGCGAGCAAGACGACGATCCCATTTTGTGATTGCTTCAGGGAAAAGAAGTATTCCAAGCGCTGCCAACAAAACGAAAAAACCTAGAGTAATACTCAGATAGATAAGTTTTTTTGCACTGATATCAAACATGAATATCGTTCTTCTCTGTAAAATAAAGGATCTTTCAGGATTTAAATGATGGTATGGTAAGCTGGAAATATAAGATAAGGATTTATATAGCTCCTCAACCTCAATCATAACGATCTTAGCAACTCTCTTGAAAGGAAAGAAGTAGTTTATCCCCTAGGCTACGGCTTTAACGGCATCTGCGATGACAGTTGCCACCTGTTTGTCCAAGGCTGAAGGTAGTATTTCATCTCTGGTTGGATTTTTGAGGTAATTAGCAAGGGCTTGAGCTGCTGCAAGCTTATGTTTTTTTTCTACTTTAGGGATTCTTGCCTCTAATAAGCCTTTGAATAGTCCAGGGAAGACAAGCACATTATTTACCTGGTTTGGGAAATCAGACCTTCCTGTTGCAATAATGGTAGCTCCTCATTTTTTCGCCTCATCAGGGGTAATCTCTGGCTCTGGATTAGAAAGAGCAAATAAAATAGGATCTGAATTCATCTTTTTTACATCCTCAGTAGAGATGAGATTTGGCTTGCTCACTCCGATAAATACATCTGCTCCGACGAGGGCTTCACTAAGAGAACCGCTGCATTGATTAAGATTAGTCAGTGCTCGAGGCTGTTTGTATGTATTAAGATCGGTTCTTCCTTGATGAATACAGCCTTTTGAATCTGTGATAACGAGATGTTTCGCTCCAAAATCCTGTAATAATGAGGCAATAGCAAGTCCTGCCGCTCCTGCTCAAGCAATAACGATTTTGAGGGATCAGATTTCTTTTTTTGTGATTTTGAGAGCATTAATGAGTCCTGCAAGCACCACGATAGCTGTACCATGCTGATCATCGTGGAAAACAGGAATATCTAATCTCTTTTTGAGTTGTTCTTCGATATAAAAGCAGTTAGGAGCTGCAATATCTTCGAGATTAATTCATCCAAAAGTAGGAGCGATATTTTCTACAATTGAGATAATTTCTTCAGGATTCTGCGTCTTGAGCACAATCGGCACCGCATCAACGTCACCGAAAGCCTTGAATAGAGCGGCTTTTCACTCCATTACCGGTAATCCCGCCAAACCTCCTATATTTCCCAATCCAAGGACTGCAGTTCCATCACTCACGACTGCAACGGACCTTCATTTCCAGGTATAGTCATAGGCTTTTTCTGGGGATTTTTGTATTTCTAAGCAAGGTTGCGCGACACCGGGACTATAATAGGTTGAGAGCTCTTCCCTTGTCTCGAGTGGAACTCTCGAAGAAATTCAGATTTTTCATTGATATTTTTGATGTTCTTGTAATGCACGAGTATAATAATCCATTTTTTATATTAAAAAAAGATAAAAAATCTGATTTTAGTCTAAATTTTCATACCAGAAATGCAAGCGGAAAAGTCTTCTTGCACGAAAGCCTGCTTTTCAAAGCGGTTAAAATGCTTTTATAGAAAAACACAAGAAGTCAAGACTCCAAAATTTAAAATTTATTTGAAAAGTTGCAAAGCTAGGCTGAATCCTGTATACTTGAGTTAAGTCTTTAATTCAGTTTTTTATACGTATGAAACCAACCTTGATTTCAAGGATAGTTCTCTTGGGAAGCGTTATGTTGAGTGGATTAGGATTTTATTTCCTTTGATGAAGTGTCCTTGCACAAAATAGTCAATCTTCCAAGGTTCCAACCGATTATTTCTCGAATTATCAAATCCAAAGAGACTGGAACAGACTTTCAGAGCTTTTTGTAGATATTGATGCAGAGAATAAAATCTGAAAAATCAATGGAAGTGCTACCTATTCTGAGCTTTTGAGCATTATGAAGAAAATTTTTCCTTACTTTCCACAGCAAGACTATAATTTCCAGCTTGTTTACAAACAATGCTTGAAAACTACAGAATCCATGCAAAGGAATCCTAATAAAGAAAATTTCTCAAGTTTCTATGAAAATTGTAAGAGTCCTCTTGCCAACATCACGACGATCATTCAAAATAAATATAGTGTAAAAGCAATTGCTAGTGCGAGTCCGATTAGTGGACCTGCGCCGCTAACTGTAACGTTTGACGCTAGAGCAAGTAGGGATCCTTCAAATGAAACGATTCCTGCTAGAAATTATTTTTGGTATTATAGGGATATTGATGGAATAGATAAACCCATTGGTTCTTGACCTTTGGCAAAACACACCTTCTCTGAGCCAGGAACCTATATTGTGCATCTGACGGTAAGGAGCTCTAATAGTGGAATTTTTGATGGTGAGGCAACGATATCCGTTAATGTAAGTCCAAAATCTGCAGTCATCAGCCTTTATGCTAATGGGAAAAAAATGCAGAAAAGTAAACCTTCTAAAATCTGAATCCAAGAGGCGAATAAAGGAGTATTTATTGATGGAAGTGCCACTTCACCGATGGGTGGGAGAGAGATTTTGGCATATAGACGAGATATTACGTCTAGAGATGGATTTAAATGGTCGAGAGAAGGCGATGGGAAACCTGGATATATCAATATTCCGCTTCCATGACAAGGAGAATATATCGTGATGTTAACCGTTCTCGATAATGAAAAAAATACTATCAGTGAGAAATTCAGTCTTTCAGTCTCTGATCCTGTTGCCATTATTCAGCAAGCTCCTGAAAAATGAAATACCTCTACCACGTATGCTTTTTGAGCAAATTCTTCTTATTCTCTCACTTCGAGACTAAAGTTTTATACTTGGGAACTTTTTGATGGAGATGGATCAAAGATTGACACGTTGCAAGGGAAAAGCATAAAAAAACAGTTTAAAAAGCCTGGAAACTATACTGTAAAACTCACCGTGGAGGATGAAATGGGACAGAAAAATAGTGATACGCTGAATGTTTATGTTGAGTCTACTCCTCCATCTCCTCAATTTTCTATTACCCCAACGAACAAACGACAGTATCCTTCAGAATTTTATCTTGATGCTAAAGCGTCTAACGATATAGATGTGAGTAATGGCTACGACCGTTTAACGTATGAATGGGTATTTTCTAATCCTAATGCCGTGCAAACTACCTCAACAGAAGAGAAAAACAAGAAAATAACCGTTCTTTTTAATGAAACGGGAAAGCACCTTGTTACCCTCAAAGTTTCCGATAATTATGGGAAAACTGAAGAAATCACAAAAGAAATCAATGTTGAATCTATTTTAAGACCAGAACTCACGATTCGTCCAAAATCTGCGGTTTGGTGAACTTATGTTTGATTTTCCGTAGCGACAAACGCAACCACAGCTCCGATTGTTAGCTATAGCTGGGACTTTGGAGATGGGAAGCCTCCTAGAGTCAATAAACTTAATATTATGAAATATGAATATCAGAGAGTTGGTACCTACAATATCAAAGTAAAAGTGACTGATGAAAATGGAAACAGTAATGAAATCTATGATAAGGTTTTCATTGGAGAAAAAGACAGCCCTATTATCGGCTATGAAATCAAAGACACACGTTATTTTACTTTAGCTCAGAATGATGTTTGTCAGGTCCCTGATGAGAAGTGATGAACAGGAACAGTTGATGTCCCTGCATATAGGGTCAATAGACAAGAGATGTTTACCATCAATACTGCACTTTCGGTTAATGCACAGGGAAATGCAAGTCAACTTAGGTATTACTTTCAAGCAAAGAATGATGAAATTGTAAAAAACCAGCAATTCTCGTATAAGTTTAATGGCGTAGGATGTCAGTATATTGACTATTCTCTTGAGGATACTTCACTGGGTAAAATCAGCAAAGAAAGAATATGGTTTAGAGTCGTTAATGCATTGCCAATCCTGGATAATCTGACGCTTAGTTTTCCTCAATATGGAAACGAAATCTGAATCTGATTTCAACAGAGCAATCAGACTCAAGATATTTTTGCACAAGGAATTGATCCGATCATCGTTAGAGTAAATGCTCAAGGGACTAGAGATACTGATGGAAGCATTTCTTACTTTAAATGGTATTATTATCCAAAATCTAATCCCAATAAAATCATAGAAACCAAGGTTACTCCTGGGAACATTCCATATACTTTTTTCTCTGTTCCGAGGCAGCCTTGAGAATTTATGTTCTGAGTAAAAATGTTTGATAATGATGATGGTTATCAGACAAGTGATGAACTTCTCGGGAATGGACCAATCATTATGTTCCCTCCTGATTCTAAACAGCCGGACATTCCGTTAGTTACACTCAGAGCTGATAAAATTAATGTAGAAGTCGGAGAAGAAGTTACCTTTGATATTATTTCAAAGATTCTCTCAGATAGATCTGATTTTGCTAAAGAAAGGACGATCCAGCTTGATTTTGATGGAGATTGAGAAATTGACCTTACTACAAAAGATGACCGTATTAAGTATACCTATACTAAACCTAGTCCTACCAGTGCTCCGTATATTCCAAAAGCATATGTTATATATAGAGATTATAAATGAATGGGCGAAAGTGCTCCTATCGTGGTGAAAAATGGAATAAAACCAGCATTGATCTATACTACATTAGGAACAACGACGCTTTTTAAAGATGTCAGCATTTGAGAACTTATCGAGAGGAAAATTTGTCTTGATGTTTCAGAATGTGAAAAAGGAAATCCTGCCTATCTCAATACAACGCTCGATAAAACCTTTAAAGTACAATATCCAAAAAGTTGAAGTTACACGGTCCGCATTACTGCTAAGGATAAGAATGGGAACGAAGCAAGCAACACTCTGAAAGTAGAAATTCCTGAGGTTACAAAATTGAATCCCCTTAGTTCTGGACTCTTTTTGATTTCTCTTCCTGAGGTTCAGTTTAGTTCTGATAAGATTCCTGAAATTTTTGTAGGGAAGCAGCTGAATAATGAAGTGCTTTTCTACCTCAAGAGTGATGAAAGTATAAAAAGTTGTTTCGTAGATAGCGATATTTCTTTTGATAGTAATCATGATGGAGATCCGAAAAATGATAGAGATTTTTGATGTAATAGAATCCATACTCAGGGCTATGTTCCAAGTTTTGAATCCGTAGTTTGAAGAGTATATTTCAAGACAAAGGATGATGATACTCAGTGGAAAACCAAGGATTTTTTGGTCAGTTTTGCTGACTTTGAGAAAGGTCTGGATGAGACGAGTAGAAAGCATTACCTTCTCGTTAGTGAACTTATCAATGGTATTGATGATAGCTCTAGTATCGCAAATTCTGACTTAAGAAGTCTTCTTATTATGCTTAGAAATAATTTGGGTGATAAGAATGCACAGAGAGGAAACATCATTCAGATTGAAGAATTTCTTGATAAAAACCAGCCTAAACCTAAATTATCTCAAAAACAGCAAGAAAAGCTGACTGATCTGATTAATACTCTTTCTGACTATGCAACACTTTCTGCGAAAGGAGTTTGAACGTATGAAGTTGCAAAAGAGGAAATTCTCTCACTACTTCCGCTGGATCTTAAGAAAAAAATTCTGAATGGTTTTGTGAGTTTTGAATCCGGAGAAAGCGGTTCTGGAGAGTCTCTTCAGGACAAAAGGCAAAAAATGCTTGAAACTATCGCTGCTACGATTTATGCTTCAGTTGCTGAGTCTGATCAGCATATTGGAGAAAACGAAATCGCCAAGGAAGACTACCAAGATATCGTAAAACCCAACATTTGTAAAATTGCAAATGAATATCAGATTTCTACCCAAATGTGTAGTGATTTCAATAAAGATGACCCAAATTTTAAAGCGATTCCTGATGCTAAACAGCCAACTGGAAAAAAAACTGGCCTTCCTGGCCGATTGAAGGTTCTTCTTTGGATTGTAGGACTGGTTGTGCTTGGATTTATCGGACTGGTTACTACCTTTGCGATTAAAGCGAAACTCAGAGAAAGAATCGAAAATAATGAAGAATAATACTTATCTTCTCTTTTAGTTGCTTGATTACTATTATTATGAAAATAAAAATCTGACTTTTATCTCTTGTATTTCTCGTGTTTGGAGGTAGTTTTGCTGTAATTGCAGGGAGCCAGCAAGGAGGACGAATAAATCGATGAAGCTCTCCCGAAGAAATTGCTTCTACAATCGCTGATCGTGCAAATGAAGATGAGAAAATTCAAGATACCAAACTGAATAAAATCAATAATAAACAGTGATGATACCCAAAGCAATACCAACTTACAAATACTGCAGAGTGGATCAGAAAAAATATCCAACCCTATCTCCAGTGGATTCTCTACATAGGACTTATTGCTGCAACGATCCTCTTGATTTGGAATGGATTTCGTTTGGTGACCAACAGTACGCTTTGAGGTGGTGATATCAAAACAGTAAAGAATAATATCAAAAATATCCTGATTGGAGTGCTTATCATGACTGGATTCCTCGTCATTATTAAACTCACAATGGCAGTGATCAATATGTTTTTTAGTCGTTAGTTCGTAGCATCATGTTTTCACGAAAAAAAATCTGATTTTTGTCTCTTTGTAGCTTGTTTTTGCTGGCATCTGGAGTCTCTGCTAACAGCTCCCCTGCTCCCAGTTTTGAGGCGGACTTTGCGAAGAAACTCACCTCGGATACTGCAGATAAGAGGTATGGAACAGAAAAGGTTTTTAGCTTTAGCTGCGTTAAAAGAGATCAGCCATTTATAGATAATGTAAAGTGTCTCTTCTTCCCATGAGTCAGCCCTGAAGGGAAAGCATGAGGTTTTTTGTGGGAGATCCTGAGATATGCAGGTATGATTATAGTTTTCATCTATATTGTCTATGCTGCGATTCAGCTTATTGTAAGCGGAAAGGATGCAAATCAATTAAAAAATGCAATGACAAATCTCCTCTATGTATTGGTAGGATCGGCTCTTTTCTTTGGAGCGATGTGGCTTTTTGGAACAGTTCTCAACTTGAATGAAGTTAGGACGACGGCTGGCTTGAGGGATAATCTCATTTCTAGTAATGGAATTCTCTTCTTTGTTCTCAGCTTTTTGAAAGGAGCTGCGTTCTTTATTGCAATTATTATGATTGTTATCACTGGCTTTAGAATGATGAATCCTCAATCGGGAGAATCGGGAGATGGTAAAAAATTAGCAAAAAATCTGGTTAATGTGATCTTTGCTCTGGTAGCAATGAAGGTTATTGATTTTATCTATTATATTGCTAGTCAGCAAAACTTTGCATCGCAAGCAGGAAATTTTATCATTCAGGTTGCCAAGTTCCTCGCTTATGTGAGTGGAAGCGTAATAGTTTTGATGATTATTTACTCTGGATATCTCCTTGTCGTAGACGGAGGGAAAGGCGAGAACTTTAAGAAAGCAAAAAACACGCTGATCAATATTGTTTTTGCGGTGGCTGCACTGTTTTTCTTCCTCTTCATCCTTTACCAAATCTTCTCGGAATTCAACTAATACCTGATATCAAGTATAAACTACCTATAATTATTTTACCGATTTTTCTCTTGCAATTCAGACTTTTTTATCTATTATAGCACTTACTTTATTTAAGTGCTATTTTATTTACTAACAAAGGCTAAAAAATGTCAAAACTCACAAAAGCTGATCGTATTCAGCAGATAGAAAAAGAGCTTGCAGAGCTCAAAGAGCGTTATCGTAGCAAAAAAGCTGAACGGGAAGCTCAAAAAGCTCAATGAGAAAAGATAAAAAATCTCAAACAGCAGATCAAAAGCCTGGAACATGAGGCTCAAATTGCGGAGAAACAAACAGATTATAATAAAGTCGCTCAAATTCGTTATGGGGATATTCCTCAGCTTCAAAAAGAGCTTAGCGAATTAGAGCATCTTGAATCCGAAAGCCATTTTCACAAAGACACCGTTGATGCGGAAGATATCGCAGCAATTATTGCAAAGCGGACTGGAATCCCTGTTTCTAAGCTCGTTGCGAGCGAGATGGAGAAGTTAGCAGATTTGGAAAGCTTTTTGAGGAAGAGAGTTGTGGGACAGGATCAGGCTTTGCATCTGGTTGCTAATGCAATTCGTCGTGCAAGAGCTGGACTTAAAGATCCTAATAGACCAATTGGATCGTTTCTTTTCCTTTGACCAACAGGAGTTTGAAAGACTGAACTGACTAAGGCTCTGGCTTCTTTTCTTTTTGATGATGAGAAAGCCCTGATTAGAATTGACATGTCTGAATATATGGAGAAACATGCGGTGGCAAGATTGATTGGTTCGCCTCCGGGATATATCGGGCATGAAGAAGGCGGACAGCTGACGGAAGCAGTAAGGAGAAAACCTTATGCAGTTGTGCTTTTTGATGAGGTTGAGAAGGCGCATCCAGAAGTTTTCAATCTTTTGCTCCAAGTATTAGATGAAGGAAGATTGACCGATAGTAAAGGTAAAACGGTGGATTTTAGGAATACGCTCATAATCCTAACTTCAAATATCGGTTCTCAGCTTCTTCTTGAAAAGATGAAAACTCAAGAGAAAAAGAAGTCTGAATCTTGACCATTGGTTTCTCTTGATGAAATGATGCCTCTTTTACTTCAGCAGTTTAGGCCTGAATTTCTGAATAGAATTGATGATATTGTGCTATTTAATCCGTTAGCTCCTGAGCAAATTCGTGGCATTGTAGATATTCAGCTTTCTAACTACTTAAAGGAACTTTTAAGTGCTAGAGAGATTCAGCTTGAACTCGATAATGATGCGAAAAACTTCTTAGCAGAAAAAGGTTGGAATCCTGAATTTGGAGCCAGACCGCTCAAAAGAGCTTTACAGACGTATCTTTTAGATGAGCTTGCCTTGCAAATTATTGAGGGGAAGATAAAAAGCGGAAGTTTGGTAGCTGTCAGAAAAAAATGAGATAAATTGGTCTTTTCTGCACAGTAAAAAGAGCTTAAGTAAGAAAAAACTCTATACTATCTAGAGTTTTTTTTGTAAAATAAGAAACGAGGGTAGGAAAAACCTCCCTCGATCCAAAGGAGTATCAACCTTCACAGGAGGATACTCGCTTCAAGTTGAATGTCCACTTTCACAAGCAAACTTCATCAATACTAGTAATTAACCATTAATTAAGCTGACTATGAACATAACTTGTGTATATATATTTTTCACACAATGTCAAGACTTTTTTTTAATTTTGGAGGAGCTGTTCAATCTGGGCAAAAATGGCATCAATCGTCTGCATTCCATCTATTTTTACTAAAAGCCCGAGAGAATCTACATACTCAAGTGCTGGCACAGTTTCTTTTTGATATTCAGCGATTCTATTGGCAATAGCACTTTGATCTGCATCATCAGCACGAACATAGAGTTCTCATCAGCATTTTTTGCATTTTTCAACTTCTCAATCGATAAGCATAGAATGAATTGTTCCACAGTTTTTACACATTTTACGCTGGTCAAGTCTCTTGTATACTTCTTCTTCTGGGATCTCAAGCTGGATAACAAGTGGCTTTCTCCCCCTCTCTAATAAAGCATCAAGGATTCCTTTTGTCTGACCTATTCTTCTTAGGCTTCCATCACCGAGAAGACCTTTATCATCTAAGGTTTCCAAAAAAACTTTAAAAAGACCTATGACGATCTCATCTTTCACAAGGCGACCGGAATTTACCGTTTCCCTGAGGTAGTTCCCGATTACATTTTCATTACTCATCAAAGCTCTGAGAATGTTTCCAGTTTCAAAGTAGGCAATCTGGTTTCCGTATTTTTCTAAAAGCTTCTGGGCTTGAGTTCCCTTTCCTGAAGCCTGGATTCCAGCGAGGATAATGTCTTTCATAAATATGAAGAAAAAAATAAAAAAAACTGACTCAAAACTTCGGTCAGTCTTTTTCTAGTGAAATGCCACAAGGAAGCAAGGGAAAATTATCAAGCCGAAGCATTTCCAACCTCAATATGATCTGGATTGTCGATATTATTATTCCACTTAACAAATTTTCCTTGTGCTTCAGGATTATAATTATTCATCGAATCTGGATTCTTCTGTTCTTTTTTTAGTTCTTCTATCTCTCTTTGTTGTTCTGCATTAATAATAGCTTGATCTTCTATTTGTTTTTGCAGATGAGAAGCCAAATCTTCCTTCATTTGTTCAGTATTTTTATTTTTTCCAAAATAAGCATCTACTGCTCTCGTCCACTTTTCCGCTTGTGGATCTATGGGCTCATTACTCTTTTGTTCAATAGTTGTCTTTTCTGCTTCTTTCCTTTGGTTAAGTGGCTTGACCTCGGCGTCTGGAATTCTATAAATTTCTGCTGTTAATCCTGACATGATATTTATCATTTACAAACTAAAATCTACTTCTCTAGCTGCATTTTAAATTTCTCCCCCTCAGCGACAAGCTGATAGGTAAATTCTACATCATTGATTTTCATCGTATAGCGATTATCGCTCTCTGGTAGGTGATAAATATTTATCTTTCCTTGAGTACTTACATGGAGAATTAAAAGAATCGTTCTCCTTTGCCCATCATATCGGAAGTTCACAGGGATAATACAGTCTCCATTCGGAGTTAATGTAGGAATTACATCCGTTCCCACCCCTTCAAATCCGAAGTCTTTTTCGTCCCTTTTTCCGCTTTCAAAAGAAAGAAAGTCTGGATTTTGGATGTCAGTTTTTACCTGTTCAAGAGCGGTCGTAAGTTCGGAAGGAGTCAGCTTTTTATCGCTTTTTTCTAGTCTTTCCTCAAAACTTAAGAGTCCTTCAAGTCCTGTTCCTGAACCTTGTTCTTGGATAAGTTTGAGGATTTCTTGATTGAGTAGTTGATTTGGGACTTTATTCCCTACCTGCTGAGTGTTTGTGGTCATCTGATTTTTTGATAGGAAATAAAATTACAAAGCATCAAGCATTGTATCTAAATCAACGATCTCCTCCTCACTCTTCTGAGCTTCTTGCTGTTTCATAGCTTCAATCTTTTGGAGTTTTTCTTTGAATTCAGCCTTCTGAACCTCTGTATACGTTGTATGGATACTTTGTCTGAAAATTTCATACAACTTCTCAAAAACCTCAGGAGAAAGCTGATTATTCTCAATGAGCGCTTTTAGCCCTCGTCCCATCTTTCGATCCGGCAGGAAATTGATAATCTGGAGCATAAACTCTTTTTTGGTCATTGCGATTTTCTGTGTATAAAAGCACGTACCTAAAGTATAGTCAGAATTTTCATTTTGTCAAAAAAATCATTGTAAAATGAACGAGCTTTGCTATACTTTCAGAGGAAATTTTCTTTAGCCTCTCGTTTTAGCCGATGAAAACTCTGATTAAAAAGATCATTTGTTATGATAAACGATATCCTGTCTTCAAAAATTCAGTTTTTTATCATGTTTTTAAGAAAGCTCAGGCAAATATTGCCACTTCACTCTATAGCAGTCCCGCAAATGGATTTTTCATCATCGGAGTGACGGGAACTAACGGGAAAACGACGACGGTCAATTTGATTCATAAAATCTTAAATGATAATGTAGCTCCAACAGTCGCAGTAAGTACTGCCGATTTGAGAATTGGGAATAAAAAAATCACCAATACCAAGAAAATGACCTCTCTAGATGTTTTTGATCTCCAATCTCTCCTCTCTACTGCGAAAAATAATGGTTGCAAGATTGCGGTTTTAGAGGCTTCTTCTCAAGGATTGGATCAACAAAGATTTGCAGGTATTGACTTTGATATGGCAGTTTTGACCAATATTACACATGATCATTTGGATTATCATGGGACAATGGAAGCTTACGCTGCAAGCAAAAAATCACTTTTTAAGAATGTACTTTCCAATACTAAACAGAATAAATTCTGAATCTTCCCTTCTGATGATAGTTATGGAAGGAAATGGTTTGAAGAAATGCCGTTTGACAAGAAGATCAATTATTCTCGTCAGGCTTCTTCTATTTTGAAAGCAACTCAAGTCGTAGAATATCTTGATCATACAGAGTTTGTCTTTTCGTATCTTTGAAAACTCTATCGTAGCTCTACAAAGCTTCTTGGTGCCTACAATGTAAACAATGTACTCGCTGCAATTGCCGTAGGTATTGATCTTGGCTTAGAAGTCTCAACTATCCTCAAGTCTACAGAAGCTTTTGATGGTGTCGATGGTAGGCTTCAGAAACAGGAAATCAATGGAGTAAGCTATTTTGTAGATTTTGCACACACTCCAGATGGATTAGAAAAGACTTTGAGCTTTTTAGCTGGTCAAAAATGAGAAAAAAAGCTGATTACCGTCTTTGGTTGTCCCGGTAATAGAGACAAAGAAAAAAGACCAGTAATGTGAGAAATCGCACGAAGGTATTCAGATGTAGCTATTTGTACTGATGATGATCCTGGGACCGAGAACAGACTGAAGGTTCTTGATGATCTGAGTAAGCCAATTCAGGAGAGGCTTCTTGCTGATGGAAAAAGTTCCTACATTATCCCAGAAAGGCAATATGCGATCCAATTTGCTATGGATATTGCACAACCTGGAGATATTGTGCTCTTGGCTGGTAAAGGACATGAACAAGTTCAACTTACTAATTTTGGGAAGAGAAAACGAAATGATAGGACTGCCCTACTAGAACTTGCAAGATCAGCGAGATAATAAGCGGAATTTACACGCACAGAAAACTTGCCAATTTTATACCATACTCTCAGCCATTCGATTGATTTTAAAGCCTTTCTTGGAGAGCTGGAGTTTTGGGTAAACAATATCCATAAAATTCCTCACTTCCCTGCCTTTAATGACTACATTATACCTATATTTTCCAAAGATTTTGTAAATTAATGGAGGTGTCGTATAGATTTCTAGGTCGTCAAGCTGATACTTCTGCTGAAGATAAAGTAGCTCTTTGTATAGCTGATCTACCTTATTGAAAAGCTTTTGCTCGATTTCATCTTTGTAGAGGATAAGGCACAACTCTCCAAATGGTGGATAGGAATATTGCTTACGAAAGAGGTTTTCAACAGCAAAAAAATCAGATTTTTGCATTTTACAAGCACTACGGATACTATGATGATCGGGATTCCTTGTCTGAACCAGGAAATTTTGACAATGATGTTTGCAGAATGCTTCATAAAGAAAGTAAAAATTTTTCTCGCTTGCATTATAATCAGGGATATTAAGCCCGATATCTGCATCCAGAATCACAAGCAAATCTAATTTCCAACCTTGCATTGGCGTCGTCAAAAGAGAAGTTCAAATCAGGATTGGATTTTTTCCTTGCTGAGTAAATTCAGAGAGTTGAGCCTTGAATTTTTGGATTTTATTGGGAGATCTTACAGTTTCGGACTCAATAATCAGACTCTCTGCTCCGTATTCTTCTTTGAGTAACTGAGCGAGCTTTTGGGTGCCCATCCCATATTCTCTTATTTCTGCACTTCAACAGTTTTCACACTTTTTGGGAAAAGTATATTGTTTTTTGCAGATATGACAGATACCAAGCTTCTCCCCACTCGGCAAAAGATGATAATTCACAGCAACAGAACAGCGATCGCACTGCGGAATATGACCGCAATGATGGCAAATTACGCCTGCGTGATAGCCTTTTTTATTGATGAGGATTCAGATTTTTTTGTTGGTCCTTAGGTATTTTGCAAGCCCCTTTTCAAATAGTTCAGAAAAATTCTGGTTGAGCTCTTTGGTCATATTTACCAGGTAGATTTTTTTTTCTCCATGATCAAAGAGTTCGTAGTTCATAATTGGAAAAATCTATTAGAATAAAATCTTTTTGATCGGAAATGAGATGAATCAGTTTTGAGGCTTTTTCAAGGATTATAGGTAGCGATTTAAAAAACTAATGCAATAGTATTTCTCTTTTTTCGTTTGAGCTTGAAAAACGCATCTGATTTCCTTATACTTTGTGGGTTATGGGGGTGACCTTGGATTTGATGAGAGGAATCCGTACCCATTGCAAGCCATTGCTCGATGATCGCTGAGCATAAACTCGGTCAAACCAAAAATGCTAACGCATGAAAATCTCTTCTTAAGAGAGCTGAACTTGTAAAGGCTTACGCTTAGTAATCTTTCAAGCCAGTTAAAACTAACCTTGTCTCTTGACTCCTGCTAGGAGCAAGGCCGATTTGAGCAGGAAACCAAAACCTTGTGTTGAAAAAATTGAAGAATTGAGTTTGTTACTTTTCTCCTTCTCCCCTCAGCATAAGGCTAAAAAATTAGGTTAAAAAGCTAACTATAATGGTAGAAAGCGGTGAGTATGCCTTTTAGACCGGAGTTCAATTCTCCGCACCTCCAAAGACGGAGATTTTGGTCAAAAAATAGAGTTAAAAGTCGCATTTATACTGGCATCTTGGGGTGTTGTAAATGTGACTTTTTTTGAATATAAAAAAGCTGTATTTATTACAACACCAGGTATTAACTGTATTATTTCTGGGTGTCCGCTAGATCGCAGTTTATATGTAGCCTTTTAGACTTCATTTAATTTTTCCCCAGGTCTAAGAATGAAGCAAGACCAGAGATGTTTCATTTTTAATTGCTATCCGTTCAATAAAATGAATTTAAACTATCTGATATTTTTTCTATAGAAATTTTATCTTCTAAGTTCCTCAAAAATTTAATATTTATAGCTTCTCGAGGATGATTCACTTCCCCATTTCTAATTAGAATTTTATCTTTATATTTTTTGATTAAATCTTTAATATCTCATCATTTAGATTTTACCAATTCTTGATAGATTTTTTTATAACATTCCTTTTGTTCTTCGTCTTGTTCTCAAAGAAAAAACTGTATAGGCAATTGTACATTACAAATCATAGAATATATTAACAATAGAAGAGAGTTTTCTTCTCAGAATCGTTTTAATAATGGTAGATCCTTTCAGTCTCGATGTATCAGATCGTATAATAATATCCATAATGTTTCTATGAATACTTTGTCATCACAAGAAATATTAGATGGAATTTTCAAGAATTTTTTGGTTAAGTTCAAAATAGAGGCTTCTTCATTATTTACTATTTTAGAATTATTTACTATTTCAGATGGAGAGAAACTTTCAATTAGCTCCTCGAGATCTTTTTTATTTTCTTGCTTTGAGTAAGAATGAGTTCTAAGAATAAGATATAAGAACATAAATTGATATACTATATCATCTTTCTCTTGAATTTCTTTTAATAACCCTTTTATCTTTTCTGATCGCTCTTTTTTTAGAGATCAATCTGGATCTTGAATATTTACTATTGGAAAACTATTTATATCTGTAAACGTTCAAAAAAAATATACAGTAGCATTAATAAATCACTTTTCTCTTTCCGTATCTTTCCAATTATGTTTTCAAAGGAGGGAGAAATATTTTCTGATAATTTGAATATAGTTGTCAAAAAAATTGCTAAAAACTCAGTGATCGTCTTTACTTCTATAGTTAGAAATATTCTGAATAATATCGTACATTATTGATGAGGTATAGGTATGTTCAAATGGAAAAAATAGTCTATTGACTATTTGCGGAAATCTATCAATATTATCGAAATTAGGCTTTTCTAATTTTTCCAAAATAGAGGTCCTCCAAGAATCAAGAATATAAATTGAAAAAGACCTAGAATCATTTTGATTACGTATTAACTTTTCTATTAAATTACATTTATCTTTTCAAGAGTTAGCTAAATTAAGATCTTTGAAAATAAAACTATGTTTATCTTTTATTTCTTTTACTTCGAGGTCTTTTGGACTCAATTCTTCAGACCAGCTAAGGTTCTTTGAATAATAAAATTTTAAATCAGATAAGATGGACGTTTCTTTTTCATATACCGATTTTATATACAAATATAATGAGAATATATCAATCCGGTTACTTCCAGAATAAAAATAGGAGTTTATACTTTCTTCTGGAATAATATCATTATATTTCTCTAAGTTTTCTTTTGCTTTATTTATATTTTGTTGAAACTTTTCTAATATTTCTTTTTTTAGAAATAATAAATCTCTTATTGTTACGGCTATATCTTTTTCTTTAAGTGAATCACATAGGTTTCTACATAAATACCGTGTTTCGCGACTTTCCTTATTATCCTTAATTTCTATAATCGAATTGGCTAGAAAGGTTGTTAGATTCTCATACGTAGTATCGTAAATATCAATTTGACTATCACGAAATTTATTAAAATATTGATAAAAATTAATGCCTTTTTCTTCCTGACGATCTCAAAGATAAAAATTATTCAAATAGTTAGAATCTGCAGAATATAAACAGATCAAATTAGGGGCAGATTTATCTTTATCTTCCTTTTGTATACTTGTTAAATTTTTTAGTAAATTTAATATTTTCCCAATATTTAATAAATCTTTCTTTTCTACTCTATCAATATCATCAATGACAATTACTACGAAATAATCTTTATAAGTAGTTTTTAGTGAGTTATTAATACTCTCAAGAATATCTTGTACATTCTCTGATGGTGAATTAAAAAATTTAAAAAGTAAATTAAAGTAAGTATTCCCTGTTTCACTTCCTAAGACTCTTAACAGATTAGATTTATAGCCTTTTAAATTTGGATCATACTTTTTAGATAATAACTCTCAAATAAAAGTATCTAAAAAATGAGAATAGATTGATTTTGTTGTTTCTAAATTTCGAGGAGAAAAATCTATACGAATAAGTTTCTTCTCTCATAAGTAATCTTTGTTTAAATAATTTAATATTTCTTCTATGAAAAAAGTTTTACCAGATCCCCACAATCAAGTAATAAAATTCATTCCCGATTTTATTGTATATTTTCCTTTTTCTTCATCTTGTTCTAATAATTGATACTCTATATAGTCCTTTATTATTGGAGAGAGATTTAAGCAATCATCAATTTTATTCATTCTTTGCATCCGGTAAAGAAAATAAAAAACAGATAAATCGTAACATAACAAATCTTAAAAAAAAACACCAATGAAAATTTAAGTATATCCAATGAAAATTTAAGCAAAATTTTTTCAAGAATACTGTTTCGGAAATTGTTGCCCAAGTTCATGAAACAGAATATGATAAAAATATCTGATATTGATAGGTGGTATATATTGAGACAAAAATTGAAAAGATAAATATAATTAATCAAAATCGGAAAAGGATCCGTCATAAAACAGACCTATTGTTCCAAAGATATTTAAGTTTTTTCCATAATAAAATTAAGACTAAAATAATAATCGTAATTAATCCAACACACTGAAAATTTGTTAAATTTAGATTAACAATGTCTATCTTAAATGATAATAGAAATAGTAATGTGGTAGAGAATAATAATGCGATAAATCCATCACGATTATTAGCGATGAAAGTTTTAAACTTTTCCATGATTAATAAAAAAAACTCAATAAAGAATTATTTTATTCATATTTTTTTAACCAGTGTTTCAAGGTATGTTATTATTTATAAACTTTATGTATCATTCCACGATACTTATTTCTTCTATGGATTTACCAAGTTTTGGGCCAAGAGATTATTTTTTTTCGCAAACGAGTAAATGACCAGTGATTGTCTTAGCATCACAAACATCATAAAGAGGCATAATCCCCGAATGGAATCAGCTTTCTTCTGCCGTTTTTGCTAATTCTTGGATCGTATGAGGGAATTGCTCAATTTTAAATTTTTCTCCGTTAATTGTCCGAGTAAAAAGCCTTCTCTGGAGAAAATGACCGATCAAAAGTTGCCCCTCTGGCTGCAAAATACGATAAGCTTCAGCGAAAAAATGCTCAGTATTTTCGATATGCTCCAAGAGAAAAAAAGCTGAAAGGAGATTGAACGATTCGTCTTCAAAGGGTCGGTCTTTCTCTAAATCACAAACGACTTTTTTGATTCTTCCAGGATGCTTGGAGAGAAGCTCTTTTGCACAGTCGCAGGCAATGTATTCATCAGGATTGATGACTTTGAGCTGATCATACATTCTCCCATCCCCTGCTCCGAGATCCAAGATACGAAACCCGTTTCTCTCTCTAGGGACAAATCTCCTAAAATCCAAACTATAAAATGAATTCAGATGAGGTCGATATTTTTTATATTCATCTTTGGTACGGTTATAGCCCTCCTCTGGAATAAGAAGTTCTACATGTTTGCTATCATAGGTCATGCATCGATTTTTTATTACTAAATCTGGCTAAAAAAGCCCTAACTGCTGAGGTTGCTCAGTTTTTTTCTTTTCTTCTTGTAAAATCTTCTGTAGATTCTTGAAACCTCGTTTCTGGACAAGAATCTCCTCCCATTTTGCGAAGTCTATACTTAATTTCATCTTTTCTAAAGGATAGTCCTGAAGCTCAGGCACCTGCATCAGCAGAATAAGTTCCTTGGAAAAGAAGGCATCCTCCTTTCAGTTGATCAGTTTTTCTTTTATTTCACCTTGGATTTCATCAAGATGAGTATAGATTTGCTCTATTGTTCCGTATTTTTTGATCAAATCGGAAGCTTTTTTGGGTCCAATCCCCGCAACTCCTTTGACATTGTCTGCGGCATCTCCAATCAACGCCAAATAATCGACAATTGACACGGGAGCAAAGCCAAACTCTCTCTCAAAATCGGGGATTCTCGTGGTGATATGTTTGAGAGGATCGGTTACGACGAGATTTGGAGCGAGGAGCTGTTTTAAATCCTTATCAGAACTAAAAACCTGAACCAAAAGATCAGGATTCTTCTGATGAAAAGAAGCTAAAGTCCCAATACTATCATCTGCTTCATAGCCAGGAGCCGTAATCGTAGGAATCCCGAGCTCGGAAACCAACTCCATAACTAAAGGGATCTGAGCTTTGAACTCATCTTCCAGCTTCGTTCTATTTGCTTTGTAGGCTTCATACTTCTCTTTTCTCTTGGTTTTGATGGGAGAATCCCAGCAAATCACAAAATATTCCGGCTGTTCAAAGAGGAGTTTCAGCATCATACGGATAAAACCATAGATTACATTCTGATTTTGCCCTTGGGCATTGATCATCGGAGGGAAAGCATACCAAGCTCTAAAGAGAAAGCCTGATCCATCAACGAGTGAAAGGGTTTTCATTGCTATTTAATACTTAATAAACTTGAGACGGTGTGTTTTTAGCTGATTCAAGACCGCATCTTTCGCTTGTATTCAAGAGCATTCTTCAGAGGCAATAAAATCCAAAAGCTGATCTTTGATTTCGAGCTGCTTCTTCTCAAAATTTTCTAATGAAAAAGGTGAATCAGACTTTTTCTCATTCCACCGTTGCACGAAAAGTCCGAGCAATTCGGCGTCATTGGTAGAAAACTGGAAAGTAGTTTTCCTTCCCTGAGCGATAGTTCCAAACGTAGCAAACAATCCCAAGCTCTGTAAACGGGAAAAACACTCAGCAAAGAGCTGTTCTAAGTTGTTTCGGATAGAAAAGAGCGGAATATGTGCTTTGGCTGAGAAAACCTGTCAATCTTTGTGCTCCAAATGCCCATACATTCGGATTAGTGCGAAGAGGAAGCTGAGGATTTCTTCATCGGACTGCGGAGCTTCCCAGTGCTTTGTAAGTCAACGGATGATCACCCCTTGGGCTTCCTTGCTAATCGTGCAGTTTATTCAAAACATTTGAGAGCTCTCTTTTTGGAGCTTCTCAGTATCTTTTGAGAAAAAAGGCAGAAACCGATACCTCTCCCCTGTTTCCTCATTAAATCAAGAAAACATTGCAAGCGTCCTCTCCAGCATGGAGATATTAAGCTTGTATTCTGGAAGCGGCTGATCTTGGAGATAGCAAACCTGCATCCCTTCTCCTAAATTCTTGAGGTTCATTAGCTTTCGTTGCCTCTTGTGATAGACAAGAATCCCCTTATCATAGCTTGAAATTTGGTAGCCGGAGAGCGAAAAACGATACTCCTTGAGCTGAGGGATATTCGGATTCTCAACACAGAGTAAATGATGCTGGTCAAAAGTAAGGTGCATAGCTATCAACAACGACATAAAATGGCAATTATTTCCCTCTGAGGATCTTGTAGAGTAAGGTAAGTTCTATCAGATTGATCATCAGTGCAGTCCCACCATGACTAATGAATGGTAAAGTAAGTCCTGTATTTGGGAGGATCTCGAGATTTACTCCAACATGGACGAAAACCTGCACAATGATCAGCGAAATAATTCCGACGGCAAGGGTTTTGAGCTGGGGATCTCTTACTTGATCGAGTTGCTTTAGCACGGTGAAGAAGAGCCCAATATAGAGAGCAAAGAGGATCATGTTCCCGATAAATCCGATCTCTTCTGAGAACGCAGCAAAGATCATATCAGAATAGGCCTCTGGTAATTGCCCCATTTTTTGGAGCCCTTTCCCGTAGCCCTGCCCTCGAAAGCCTCCTCCGCCAATGGCGATGATTCCCTGCTGGTTTTGTCGGCCATCTTTTTTGGAGATTTCCTTTTTGGCTTCCTCATCGGTGGTGAAAAAGGTTTTAAGTCTGGTCAAGGCATAGTTGTCGGGATTCCACATACTGATGGTTCCTACTCCGATCAAGGCAATCACTCACGCAACACCAGCAAGCAGTCAGACTCTTGCGATTTTTAAGCCATTATACCATGCCATGATCGTCCCGGTCAAAGCGAGAATGAAGAGCGTCCCCATGTCAGGAATCGCAAGGATCACCAAAAGAATAATCGCATTGATCAGAGCAAACTGGGTCAGGAACGGTGCTGATTTGATGACCTCTCTCCTCTTGGAAATCCAATAGGACATAAAAAATACGTATCCCAGCTTGAAAAACTCCGCAGGTTGCATATTCGGGAGTCCAGGGAAGTCTACCCAGCCTCTCGCAGTTCCGTTTGTTGCCTTGATCCCGGGGATAAACACACAGAGCTGGAAGAGAAAAACCGCCACTGCAATCAGAATTGCTAGCTTTTTGTCCTTGAGTAAAGGGAGCGGGAAAATCAGAGTCAAGAAAACGACAATCAATGCTACCGCAAGATTCTTGATCTGACTGGAAAAATAGAAGTAGTTATTCTTTGCTTGGATCCTGGTTTCTTCGTTTGCTACGAGTTTTACCTCCTCGCCGGTCTCCGCCTCAATCGTCGCAAGCGTAGGCAAGACAGGGATTGCCGCTGGAGCCCCAGAACTTACCTCGGCTCAGGAAAGGTTGGACCCCGTCGTGTCAACCACAATCAAAGGTTCAGACTTTTGTATGATTTTTTGTGTCGTTTTGAGGATAGTTTCATCGAGAAAGGTGTTGAACGCTTTGACGGTTTCGATATTTTGGATGATCGTCACCTTGAACCTCTGATCAAAAGACTTCCAAATACTTGTGCTATATACCGCAAACAGTCAAAATAGTGTCAACAATCCCCCGAAGATCCAAACTTTAAAAGCATTATTCATAATGTTGGATTAGTGATGTAAGTTGGTGGTCTTACTTTTATGATTTTGGTGGGAGATTTCAAGAGAGAACGCTGATTGCTGTCCCGTATCGTCCTTGACATTGGAAGGGTTTAGGATTTTGAACTTGAAAAATCAAGGGAAATGGGTATAGTCCCCGTAGTTTTAGTTTTTTTTTGAATGGAATGAAATTAGGTGTCAGTGTTTTTGCTCTTTTGGGAGTTGCTTTGCTTGCGGGGTGTCAGCCTACACAACCAAGTGAACCCACTCTACCACATCAGCCTTCGGTGGTTGTCAAAAACGATACTCAGGCGTTTAAGACGGAATACGAATCACTCAATGCGGAAAAGATGCCGATCACCGTGCCTGAAGATTTGAGCATTTCTGTGCTTGATTTCTCTGGGACGAAAGAATTTTTGGAGAAAGGGACAGGAATCCTTTTCTTTGGATTCCCAACTTGCCCATGGTGTAGAGCCTTACTTCCAAACCTCTTTGATGCAATGAAAAAGAGTCAGATTTCTGATCTTTATTACTACAATCCTAAAGAAATCCGTGACCAAAAATCACTCGGAGCAGACGGAAAAATCGTTGTTGAAAAGGAGACCAGCCCAGACTATCAGTATTTGCTCGAGAAACTTGATAGCGTGCTTCCAGCCTATGAGGGATTGAAGGATCCGAGCATCAAAAGGCTGTATGTGCCGTTTGTCGTGGTGCTGAAGGAGGGGAAAATCGTGGGATCTCACCTCAACACGCTCGATGAGCAAGAGGATCCTAGCGTTCCGCTTACTGATGAGCAGAAGGCGAAGCTTGTGGAATTGCTCGAGCAGGCGATGGCGCCCCTTGCCTCACAGACGTGTACTCCTGGAGAGCTTGCTTGCTAGGAAAAATGAAAAGACTGAGCGGCGTAAGCCCTCAGTTTTTTTTGTTGGTGGGAAAGTCATGGGATAGAAGTTTTTGAAAAAGAAAAAAAATCTGACTCCTCCCCTCTCGCTCCTCCTGATCTTGACCCCCAGATTTGATGCCCGGACAGGTCGGTGAAGCAAGAATGACCCCCAAATTAGGGTGTCGGACAGGTCGATGATGCAAGAATGAGCCCAGATTTGGGTGTCGGACAGGTCGATGATGCAAGAATGACCCCCAAATTTGACTTCCGAGCTGCCGGAAAGGCGAAAATGCCCCCCAAATTCGATGCTCGGGGTGGTCGGAAGGGTAAAACGGACCCCCAAATTTGATGCTCGGGGTGATCGGAAGGGGAAAACGACCCCCCAAATTCGATGCTCGGGGTGATCGGAAGGGTAAAATGGACCCCCAAATTCGATGCTCGGGGTGGTCGGAAGGGTAAAATGACCCCCAAATTTGATGCTCGGGGTGGTCGGAAGGGTAAAATGACCCCCAAATTTGATGCCCGGGGTGGTCGGAAGGGTAAAATGATCCCCCAAATTTGATGCTCGGGGTAGTCGGAAGGGGTAAACGGACTCCCAGATTTAAACTGATGATTAAACGCCCACTCTAAAATAGATCTCTTTATCAACGAGTTCTCTTTTGGTTCATCGTTTGAGGGAGGAAATTTGTTTGATGATTTGGACTTTCTCGGGAGAATTGGCGTAAGGAAGGCCGTCTGGGAAACCGGGATACAGCACTTTCGCCTCAAAGCTGAAGGGTCTTGTCTGCTGGTTATTGACAGAAACCTTACATGCGCCCATAAAGGCTGCTGTAGAGGTCAAATCGGTTTGGTTGAATTCAGGTTCAAACTCTTTGGTAAGGAATTCTGCGTCGGGAGCTCCGACCTTGAAGACGAACATATTTCCGATATTACCAAAAATGGTCTTGGAGAGGTCCATATTCCCGCCGAGTCCTTCCTGCTTCAGCTGGTCGGTATACTGATGGGCAACCACGAGTCCCACTTTATATTTTCTCGCCTCTGAAAGAATAGATTCAAAAGACTGAGAGACATAGTTTTGGAACTCATCCACATAGAGATAGTGAGGAACTCTGTCCTTCTCATCGATTTTAGCCCTCTTCAATGCTGAAAGTTTTACCTGCATAGCGATAATCTTTCCAATCAGTTTTGAAGTTTCCTCTCCAGCGATACCTTTGGCGAGGTTCATGAGAATGATTTTCTTTTGCTGCATTGCATCATACATTTTGAAGGCGGATTTTGGCTGTCCGATGATATTTCTCACATACACACCGGTCGTAAATGGTCAGAATTTTGCCTGGATGAAAGGAATAATTTCTGCTTTTTCACGGTCCCCCATCCTTTTGTAGGTTTTATTCCACCAAGCTGCGACTACCGGGTCTTTGAGGTTTCTGATTTTTGCTTCGGCAAAAGCATCGTCCGTAAAGAGTCTCATAATATCAACAATGGTGCCGCCTTCGGGCTGATCCATGAGTAAAAGGCATCCATTACGGAAGTAATCCTGGATTCTTGGTCCGAAAATTTCTGGTCCATACATTTGGATAAACATTTCTACCAGGTCATTAGTCACGACGTCCTTTTCATCGCTGTCCTCAGGGTTAACTTCGAGCGGATTGAGGCCGATCGGATAAGCGGTATTCCCCAAATCGAAATAAATGAGGTCATCGATCCTCTCCTTAGGGAACCATTTCAAAAGTGCTTCACAAGCTTCTCCATGGGGATCAATAAAAGTAAAGCCTCTGCCGTTTTTTATATCATCGACTGCCTGAGCAAGCATAATGGTAGACTTTCCGGTTCCTGTCTGACCGAGGATATAGATATGTCTGAATCTATCTTTGTCGGTGATTCTGATTTCTCTTTTGATGCCTCAGAAGTTATTGTAGCCGACAACGATTCCTTCGCTAGGAAGGTCGTCTGGGGCGGGAACGATCTTGAATTTTTGCCAAGCGATTCTTGGGTTTTGGTTGAATCTGCCGTGAGGAAAGTGAATAATAGAAGAGAGTTCCTTGATATTGAGGATCGTCGTCTTATTAAACTTTCGGATGTCGTCCAGCAAAGTATTATCACTATAAAAAAGTCTCATCACATAGTGTTTAGCAAACTTTCTGAGGTCCTGTGCCTTGGTAAATTTGATGGTATTGAGTCCGATATAGTTATACTGATTGAAGAGACGAGCGATGTCGTCCAAGATTTTCTTGGGACGGGCTTTGTCTGGTGAAGTAGCGATCGTTCTCATCTTCACCTGGAAGAGTTCGTCATCCATCTTCTTGTCAAAATCGCTCAGCTGCTGCTGGGAGAAGTTGTGTTTCTTTTGTTCTTGTTCCTTTTTGGATTCTTTCTGCTCCTTTTCTCCGCCCGCATTTCGAATTTTGCTGAGGATGCCGAGAATGCCCAGCGGTTTTTTACCTTCTTTGATCTCGTCGGCTCTCTTTCTCATCTTTTTGAGCCAATCCTCGTGGAGCGGTTCGACCAGGATTTGGATTCAGACTTTTTCATCTCTAGAAATATTAGAAAAAGCTGACAGAATACTATCCATTGGGTCTGCCTCGAAGGATTCATAGGTTTTGAGCGGATGAACGCTGTCTTTGGTCAGGGTAAACTCCCCTCCTGCGAAGAATTTTCCTGCCTCCAGGAGTTTGGGCTGCTTGACTTCTCTGACGATTGCACCGACATAAAAGGAGGCGATCATTTTTTCCACGGTCATGAGGTGCTCCCGTGGCACACCGAGGTAGAATTTGATCACCTCTTTTTCAACGATGATTTCCATACTGATGAAATTATTTCCGAATCGTTTATTTTCTCGCCCTTGATCGAAAATCGCATAGAAATTTTTGTACACCTGATTCATTAGTTCGATATTGTGCTTCATTGACTGGATATGATCCTTCGCATCAATGTCAGAACTCCTCGCTACTGCATTCTTTGGGATCTGCACCTGAAGGAATCTGATTTTTCTGGATTGGTCGATTCTGTGTTTTCTCTGAAATGACAAAAAAACAGCTCTAGCAACGATAACCCCCGCAATCCAGAGCACTCCAGCAGCGATAACGCTAATCAAAAGATAGATTAGATTTTGTTCCATTCGTTCTGCATGCTAAAAAGCTCCTCTAAGTATAGCGTTTTTGGGTGAAAATGCAAATTTCCTGATGTTTGTTTGTGTCTTTGTGAGCTTAGTCCTCGTCCGTGGATTTATTTTTGCTTGCGGTGAGTCTTCGAGAGTCTTTTTTGTCCAAATAGGTTTGAATTTGCTCGGGGGTGAGCTTCTGCTCTTTGACGAGCGTGTTGATTTTGTGGTAAGGGAGATCCATTACCTCGTCTAAAAGCCCCTGCTCTGCAAGGAACTCTTGGAGAGAAGCTTTATCTTTTGCTAGGTAGGAATCAGTTTTTTTGATGGTTAGTTTATTTTCATCTCAGAAGAGCTGCTCAAAGCCGCCTTTGTCTGCGTAAGTGATCAAAATTTCTTTTAGGCTCTCTTTTTCTTTGGTAAGTTCGGAAGCTTCACGAGAGATTTTGGCGTATTGATCGACAAGTTTTTTGATAGTTTGGGCTCAGAGCGATCCTCACTCCACGAGTTCATCTCCGCAGCCGAGGTGCTTCCAAAGCGGGCAAAGCTGTTGATATTCGCAGTATTTACAGTAAGGGTTTTGCTGAGTCGGAAATGCATTTTCCCGTCCCATATTGTAGTTGAATCTGGCTTTTTCTATAGCATTGATGGCAGTGCGGTATTTCTCGAGGATTGGGGCAAGTAAAGCATCTGTGATCTCCCGCTCATCCCTCAAATCAAAATGCAAGTAATGAAGTCTGGCTTTAATCTTTTGAAAATATTTCCCATACTTCTGCTGGACACCAAGTGCATAGAGCGTGAGTTGTTCTCTATATTCTTCTTTTTGCTCGGGAGGGAGCTGTTTATTGGTTTTGTAGTCGTTGATAACGAAGGCATCGCCCTCTTTATCCAATCTGTCGATGATTCAGCGGAACTTCTGCTCTCATCAGTTTTCTTTGGATTGTAATTCAAAATTCATAATCAACTCTGTCGCAATGACCTTGGTCTCTCAGAAAGGAGCATAGGTTTGATAGTAATTTTCAAGGTACTTTTCTCCTCTTCTTAGATAGTCTTCCTCGTGCTGCTCCCCTTTATAGATGAGCTTATCGCCCGCCTCTGCTACTGCGTTCTGTCGGAGCTCGTGAAATTTTCCTAAAAGGTCGGCTTTGATCGGGATATTGAAAACGTTTACTTGCTGATAAAGCCATTCTAAAGCTCAATGCACACAAGTTCCAAGCATAAGGTCCGGAGTCGTTTCAAATTCTCTTTCGAGCTTATCTAGGTAGCGATATTGGTATTTCCTAGGGCATTGCTGATAGAGATTGATCTGAGAAAAGGAATAGACGACCATAGGGAAAAGGGGAAAGAGGTAAAAAATCAGATTTTTTTCTTTGAAGAGAGTATAAGCTTTAGAACATCAGAATCAAACCGAAATAGCACAGGAGAAAAATGTAATCATACTATAGATTAAGAAATGTCCAGAAAGTGGAAAGAAAGGCAGTTTTTAAGTGTCTTTATATCGTTTTTAAGCTAAATTTAAGGTTGCTGATTATACTCTGCGCACTTCTCTGCAGGAAGCCCTCAAGATTTGAGGTTTTATCACTTTATACGATGATATGCATAAGAAAGCAAAGTTATTAGCTTTAGTTGCTGTAGCTTCAAGTTTGGCAGTAGGAGCTGGCTCACTCGCAGCTCCGCAAACGAATCCTACCACTATCACAACAAAAACGGCAACACAAAAGAAGGATTTTAAAGAGCAGCCAGGCGGTTCTAGAACAGAGCGTCCCATTAAGCTCTCAGAAGAATTCTGACTCACAGCAAGCCAAAAAGCGGAATTTGAGAAGCTTGCAACGCAACTTAAGACCGAAAGGAAGGCTTACCATGAGAAAAAGAAAGCTGCGAGCACCCAAGAAGAGAAAGAGAAGCTCAAAACTCAGCGAGATCAGAGAAAATCTGAGCTCAAGAATCAGATTTTAGCTTTAGTTCCAGCAGAGCAAAAGAGCAAGGTTGAATTACTCCTTTCTAAGCCTAAACATTGATCAGATAAGGGTGAAGGAAAAGGAAAAGGAAAGGAGCAAGGAAAAAAAGGAAAGAGAAAGTCTGATGCAAATTGAAGTTCAGCTTGAACCACTTCACCAGCAAAAAAGCAGAAAAGAACTACAACTTCTGCTCAAAGCTAATGAGAAGATAAAAGAGTAAAGGCAGAAAACAGGAGTTTTAGAAGTTCTCCTGTTTTTTTGTAGGTCTTGAAACTAATGAGGAGAAAGCTTTTGTTTTTTTATTTAAGATAAATTTAAGGTTGATCTGTATACTGCCGATGATTTACTTTCTACTCCTTAGAAAAAATGAAAAATCAAGCACCAGAAACGACTTTTGAAAGAAGAGAAATCAAATATCTCCTCACCACTGCACAAAAAGAAGCTTTTATGGGAATAATCGAAGGCTATCTTCGTCCTGATGAGAATGGGAACTCAGGAAGTTATCGCTTGATGAGTCTCTATTTTGACTCTCCGAATAAGGATTTTTATCGGGCGAAATGGTTTAATCTCCCTTCTAGAAAGAAAGTCAGAATTAGGAAATATCATAATCCAGAAGAGAAGGGATCTGGAGTGGAGGAACAAGTCTTTATTGAGATCAAAGAACACACTCCTACTATTAATTACAAGAGAAGAATTCTTCTCCCTGTTGGACAGGCGTTTTCGATTCTCAATGGAAACATGATTGAGCAATCGCTTGCTCCTGAAGATCAAGCAATACTTGATGAGATATGAGGACTCGTAAAAGAATTTCAGCTTCAACCAACTGCTGTGGTATTCTATCACAGGCAAGCCTACAAAGTGAAAAAAGAAGACTGAGGACTGAGGATTACTTTTGATCAGAACATCAGGTTTAGGAGTGATGAGCTAAGCCTTGAGCGAGGAGATCAGGATAAAAGGTTGATTGATGAAGGAATGGAGATTATGGAGATCAAGGTGAAAGGCACACTCCCCTCTTGGACACAAGAAGCTCTAGAAAGATGTGGCATTGGACAAGCAAGCTTCAGCAAGTATTCCCACACGATTGAAAAAAGCTTTGAGTGAGCGCTGGTTCCGCCTTGTGGCTTGGAAGACTATGGGAATATTTTGAGAGAGGCGAAGGAAGCCTCACTACCATTGGAGCTTTGGAAGCAACGTACTGTATTCTTAGCAGATTAGCATTTGGAAGTCATGTTCGAAAGGATTTCTACATTCTGAATTCAGAATTTTATCTTTTAGCATAAAAAAGAAGCGATGGATTTTACGACAATTTTTACAGGGATCCAAGAGAGCTTGTCGGCCACGATGAGCAACTATAGTTTATGAGATATTTTAGCAATTTTACTCGGAACTTTTGCATTATCAGTGCGGGTTGGGCGATTGTATAGGCGAACGCATAAGGGTATAAACTACAATCAAGGTTATGTTCAGACTTTGGTCGTGATGGGTGGAATTATTGCGATGATTATGCTGATTCTTGGCTCTGATGTAGCGAAGGCCTTTACGATGATGGGAGCATTTTCGATTATCAGATTTAGAAATAATATGAAGGAAACAAGAGATATCGGCTTTATCTTCCTAGTGATGGCGATCGGAATGGCAATGGGGACCAAACTTTATCTCCTTGCGGCACTTGGAGCATTGGTGATGTCTGCGATTATCTACGGAATGTATCGCTTTGACTGGTTTGCTCAGAAGAAAAAAGGAAGAATCCTGACGATCCGCATTGCACCAAAGAAGTTTGAAGATGGATTGTTTGATAAAATATTTGAGAAGTATGGAGTCAAAGCTGACTTTCTTGGCTATGAAGGGGTTAAAAAAGAGGATTTAATTCAGATTTCTTTCAGTGTTCAGATTGGACAAAAGACCAATGTTCCTAGTCTCCTAGCTGAATTGCAAGAGAGAAATGGGAATGAGAAGATTAGTCTCAGCTATGCTTCCTAGCTTTAAATGTTTAGAGGCTGCGTGCTAAAGATTAGAAGAAGTGAATAACTCTTTCGTAGTTTGTCAGATTTAGAGCTTTAGAAAAAGGCCTCTATGAGTTGCATAGAAGCCTTTTGAATGATTACTAGTTAATTTTTTTAATACCGCATCCAACCGATGATTGAATACTTTGGTTCCGGAGTAACAATGCAGCCGCGCATTACATTGCCTGGTTCAGGATCAATGATGCGATTCCCTGAGCGTTTGAACTCTCCACCAATTGGGTACCAGTAAGAGCTCTCTACATTGACCGAGAAATTCTGCTGAGCTACCCTTATGGACGTATCAGTTACCTCCATAACGATGGCTACATGCCCATCCCCTTCCTCAGAGAATATCTGGCTATAACTAGAGAAAACCAGAATATCTCCAACTCTTGGAGCAACATCACCACCGTTAGGATAACGGCTGAAATGCTTGAAGTTGTTGGTATCAGCATACCAATCTTTTGGCTTTGAAGCGTTCTGAATTTTCTCCTGATATACCTTCTCGATATAACGGAGACAGAAATCTGCGCTTGCATTCTCTCCAGTACAACGGATCTCTACTCCGTTGGCAGTCCCGAATACTTCACCCTCTCCACACACATTCGCATAGAGCGGATCGGTATAGACCAGAATCGGTTCTGCATAATAGCATTCCCCCGTTTTGGCGTCCATAACAATCGGATAGAAATGCTCGTATCCGCGAGCAAACCTAGCATGGAAATAAGTAAAGACTACTGATTCCCCTGCCGAGGCCTCTTTTGTCCAATGGACTTCTCCATAGACAGACCCGGATTTGACGCCGACAACTACAGGATTTCGAAACTTCGATCCATCTTGCTTGGTGATATCCAAGAAAAAGACATTACCTTGTCTGTGACTGATCTTTGCCTTGATAACGCCTCCTTGAAAGATTGCAGCGGCTCCTTTTATCGTCAGCTTCCGCGAGCCCATCGGCGTATACTCGCCCTCTCTCGGTATGCTGAAATAATTCGGAGCATGGGGATCAGGTCCCGGAACAGGCGCATCATCAATTCTATACTGGGGAACTACAGGAGCTGCGTCCTCTTTTTCGCAGGAGCTCAAGAGGGCTACAACCGCGAACATCATCACTAACAATCCGAAGATTGAATTTCTGAATTTTTTCATTTGTTTAATTTTTTATTTATGGGTTTGATACCTTGGATGGAAAGGTATCGGTTTGGTGTGGTTGTAAGATAAGCCCATCCTCTTATCTTGTTAGGGTCAGAAAGGGATCTCTCTGTGAGTAACAAAGCTCCTCTAACCGAGCTTCGGAGGGCTACTACTTAACGAATAAGAATAGACCTCATGTCGTGTTTTTTTTCATCTCTTTTTATTTAGCGATGATGAATATATCTTTTTACTACGAAATGTCAATACCAAATCTGGATTTTATTTTACGACCGCTGAGAGAGGATCAGATTTTTTTTCTTTTTCTGAAAGGAGCGGTATTATGAAGAAACAGGTCTTTCTCTTCTGTTGTAAGCGTCTCTCATGCTGAAGGTCAAGAAGTAAAACGCTGAACGTCAAGAATTTTCACTCCGAACGTCAGGAAGTTGATTTATTGACGTCAGGAAATTTTACTCCGAAGGTCGAGAGTTTTGGATGCGGACGTCGAGAAAAATCAAGATAAGCTTTGAAAAGTTTTTCATTTGACTTTTGGTAAACTTTTTTTACACTACGGCTGTCATTATACGACACTTTTACTTTTTATACGAAACACCGATGAAATTCAAACTCGTAGAAAAAGCAAATCCTATGGATAGGACCAAGAAAAAGCGGTATGCTAACGCAGTCAATGCTGGGACGATGTCTCAGAAAGAAATGGCAAGGCTCTTGGAAGAGAAGTCCTCGCTCACGATTGGAGATATTTCCAATGTGATTGAAAACTTGATCGTAGAACTTCCAAGGCAACTGATAGAAGGGAAGAGCGTAAAGCTTGGAGATTTCGGTTCGTTTCGCTTGAGCCTTTCAAGTGAAGGGGCTGAGGACAAGAAGAAGTTTAACACGGCAAAGATTGAACCAAAGGTGATCTTTACGCCATCGACTGAATTTAAAGAGAAACTTCAGAAGATTAGCTATACCCAAGCTGAATAAGAAAAGATTAAAGGAATCAGAGAAAAAACCTGACTTTTTACGGTCAGGTTTCTTTTTTTTATTCAAGGATCTTAACGCCTTCTCCATCAACTCGTAACGCTTGAGTATTGGAAATCGCTTTAAGAGTCAAAGATTCTTCGTAACGCGAGATAACTTCTTTGGTTGCTTTTGCGAATGGGAAGCATCAGAGGTGAGGAACTGTTGAAAAATCCACCACATTCAGAGCTTTAAAATTTGTCAGTTCCTTTCCCTCATTTGGATCATCCATCAAACAAACATACTCAATATTGGGCGCTGTAATCACTGCACCAGCAGACTCGCCAATATAAAGTTTTCCCGTAAGGATATGCTGTTTAATTAGTTCATCTACACCAGTCTTTCTGAGTTCTTGAAGTAGATAAAAGGTATTTCCTCCTGAGATATAGATACAATCAGCGTTTTCGATAATTTCCTTTATTTTAGGAGCTTCTGTTGTGTTGATATTAAGCTCTGTTATTTGCATTCCTAGATCTTCTAGAGCTTCCCACCCCTTTTTTACATAAAACTTCACTTTCTCTACCTCTGCTGCTGTCGGGATAAAGGCAACTTTCATACCTTGCAGATTTTTTACGCAGTCTGGGAGAAATTTTGCTACATCTGCAAATGAAGAACAAAGAAAAAGCTGAATCATCTCTCCTACGATAAAAAATAAAAACTTCACTGAATGATTATATGAAAATACGCTGAAAATACAAGCTTATTTACAAAAAAATCTGACTTTCATCGAGGAAAATCAGACTTTTCTTTTATTTGAAATACTTAGAGGTCCTGCATATCAAGGACAAAGCGATAGTGAATATCTCACTTCTCTAGCCTCCCAAAAGCCTCATTCGCCTGAGAGATCGGCAAGACCTCCCCTTCTGCTAAAATATTGTGCTGGTAGCAGAAATCTAACATCTCCTGCGTCTCTCTAATGCCTCCAATATTTGAACCAGCGAAGCTTCTGCGACCATGCACGAGGTTAAAAACTGAAACCTTCAAGTGCTCGACTGGCAATCCGACCATCACGAGCGAACCATCTGGAGTGAGTAATGAAAGGTATTGATTGATATTATGAGGTGCTGATATGGTATCGAGGATAAAGTCTTGCTTGGGTGCGGATCTGAGGGCTACATGATCGGTAGTTAAGACTGCGGCATCAGCTCAGAGCCTTTGGGCATCAGCAAGCTTTTCTGGAGAGGTCGTAAAGACGGTCACGGTTGCTCCCATAGCTTTTGCGATTTTGATAGCGAGATGTCCTAGTCCACCAATTCAAAGGATTCAGACTTTTTTTCCTGGTCCTATTTGCCAGTGCTTGAGGGGAGAATAGACGGTAATCCCAGCGCATAGGAGTGGAGCTGCCATTGCGAGATTCTGGAAAGGTGGCATATGGAGTACGTATTTGTCTTCGCAGACATAGGTTTTGGAGAAGCCTCCGTAGGTCATCCCTCCAGAGACTTTGTCTGGACTATTGAAGGAGAAGGTTACTCCTTTTTCGCAAAACTGCTCCTCCCCTTCTGCGCAATATTGGCAGTGCTGACAGGATTCTACAATACATCCAACTCCAACCAAGTCTCCAACCTTAAAAGCAGAAACCTGATCTCAAATAGCAGTTACTCTCCCAACAATTTCGTGTCCTGGGACAAGTGGGAACACTGCGGTTCCTCGGTCATTTTTGATAGTGTGAAGATCGGAGTGACAGATTCCACAGTAGAGCACTTCAAATGCGAGATCCTGAGGTCTGAGTGCTCTACGAGTGATTTCCAAGGGTTTCATGGGGTGATTAAGAGCTGGAGCTCAAAAGGCTTTTACTTGCATCGTTTATGGGTATGAGGTAAAGTTATTGATTTTATACTAGGATTTCATCAGTAGAAAGCAAGAAGAAATCAGATTTTTATCTTTTTATTATCAAAGTAAGAACGGAGGGAGTCTGGATTTTTAATCTTTTAAGCTAAATTTAAGGTAGATGTGTATACTGCCGATACTCAGCTTTCCGTCAGCGTAAGGGGAAAGCTCTGGAAAGCTGGGATTGTCTTTACTTTTTAACTATGAAATATGATGAAAACAATACCATCTAAAATACTCCTAATGTGAGCGATAAGTTTAGTACTTACCCTAGGGGGATGTGGAACTACAAGTTCAACAATAGCTTCAACCAGCTCAAGCACCACTACGAGTACCGTTGCACAGACAACAAGCTCTAACATAACACTGAACGATCAAAGCGTTAGCATTACACAAGCTGGGACCTATACTTTGAACTGAACGATCAGCAATGGGCAAGTAATTGTTGATGTTTGAGAAGGAAATGAAGTCAGATTTCAGCTTGCTGGAGTAGATATTACTTCCAAAGAATCCTCTCCAATCCTCATAAAGAGTGGAAAAGCGACTATTGAACTCGTCTCAGGGACAAGCAACACGCTTACTGATAGCGTCTATAATGAAAATGCAGATGAGAACGCAACTATCTATGCTGAAGAAGATCTCGTGATTGAAGGAGAAGGAAATCTAAAAATCAATGCAAACGGAAATGATGGTATCACCTCAAAAGATGATTTGACAATTAAGAGCTGAAATATTGAAGTTCAAGCTGCTGATGATGGGATCAGAGGAAAAGATAGCTTAACAATTGATGGAGGAAAGATTAACGTGATAGCTGGAGGAGATGGATTAAAATCTGATCATGAAACGCAAGGTAGTATCGCAATGAATGGTGGAGAAGTTAGTATTTCAGCTGGTAGCGATGGAGCCGAAGCCTATAAGACTATTACGATCAACGGTGGAAAACTCAATATCCTCAAATCCTACGAAGGATTAGAATCTGAGATCATTATGATCAATGATGGAGAGATCAGTATTGTCTCTAGTGATGATGGGATCAATATCTCTGATAGTTCTTCAACCACCTCTGAAGGCATGATGCATCGAAATGGTACAGCATCAGGAAGAATCCTGACAATCAACGGCGGAAAGGTTACGATTGATGCTGGAGCTGATGGACTCGATTCCAACGGGGCTATTGAGATGAATGGAGGAACGGTAGTGGTCTTTGGTCCAACAGATAATGGGAATGCGGCACTCGACTACGATGAAACCTTTACGATGAATGGAGGAACCTTACTCGCCTTTGGAAGTAATGGAATAGCGATGAATGTTAGCAACTGAAGTCAGAATTCTGTTCTTATTGGGCTTTCGAGTGAACAAAATGCTGGAGCTAAGTTCTCATTAGTTGATAGCGATGGGAATACGATCCTTGAAACAACACCGACTAAAGCATGGAGTTCTGTAGTGGTTTCTACTGCCGGTTTGAAACTCAATAGCGAGTATCGTTATCTGGTTGATGGTGTAGAAGCTGGTAGCTTTACTCTCACAAGCTCGGTAATGTCTGCCGGGACAAGCTGAGGTATGATGTGAGGACATGGCGGTATGTGAGGTGGAAGACCTAGCGGGACTCCTCCTCAGAGACCAAGCTGGTAATAAAAAATATTTGAGATGAGAAAAACTCCCTCTGAAGGGAGTCTTTCTCGTATAAGATTTTGAGATAGAGAAAGCAAAAATTCTGTATGCATTTCTGAATGTAGACCGAGAACTGGATGTTTCTGGAGAAATAAATTTTAGGCTATCTGAGATAATAATACAGGTAAAGCACTTTTTTCAATATTTTTACTCGCGGTAAGTAAAGTAATTACCTTATGAGCTTTCAGCATCTCACAAAAAGCAAGGAAGGAACTATTGTGATTGAGTTCTTCTATATAATCCTTGCTAAAAGCCTCAAAATTACCATCTTGATGGTAACGTTTGCGCAACTCAGTACTAGGAGCAATCTCTTTTGCCCACAGATTTAAACCAAGATTTTCTTTTTTAATACCACGAGGCCAAAGGCGGTCTGCAAGTACTCTAAAACCATCTTCATGACTTCTCTCCTCATAAACACGTTTCAGATATATTTTCATCCTCTGTTTGTATTAAATAAAGATAAAGGTAGTTATAAAGTAGGTATTTCCTATTTTTCTCTAGTAATTCTAAGGCTTTATGAAGGCGGAAATCTTGTATAATCACAGAATCTCTATATCATCAAGCACAAAAGAAGAGGAATAAGAAAGACTGAATCTATCAGTCCTGTTCTACTCGCTCCTCATAGTGAGCCTTAGCTTCAGCCATAAACTCCTTATCATTCCAGTTCGGATCTTCATGGCCGGCATAAGCAGCATAAGTTGAATGAAGTCCTGGAAGGTATCCGTCCTCCAGGTCTTCTTCAGTATACCAAGTCCTCATCTCAATATCTCTCCCTCTTGCTCAGAGAAGGGTTTTGATTCTTACTCCCTCCTTAATGAGAGGCTCCTTATCTGCATTGCGAGAACAATAAATTCACTCTATCTTGTGCTTATATTTTTTTCTATATCGCCAAATCAGGAGATCTGAATTTACTTTAATCATTTGTAAAGGTCTAAATAAAACTCTTGTATTGCTCTAGATAATTTTAATATGAAGTATAGATAATAGAGTAAGTAAGGAGATATTGGTTATCTCGTAAACGATATTGTAGAGCGATTGTTGTTAGAATAGAGTTAGAGGGGAAAAATAAATCGTAAAAAATAGCCTAAACCGCTGATAACCACAATTCCAAATGAACTGAATACTAAGAGAAATAACACAGATAAGGAGCCATTACTAGAAAAGTCTCGCTTCTTAGCTTCTACTCAAGAATAATACTTCTTGTATAAGAGATAGTGCATAATATACATAGTAAAAAGTATTAGATGCACAAATATGATGAAATTATAAATTTTTACTTCGGGAAAGCAACTAATTTCAGAATGGATATTGTAACAAAAGTAGAAATCTTATATAGTTTCTGAACTGATCTTTTTTTATTAAGCTTCTAACTAAAAATCCAGTTAAAGTCTATTTTTTCTTTCTTCTAAAAATCTCTTGCATTTTTTTAAAAAAAGAGTATAATCCTTATACCATAACCATAGGTTATGGTATATTTATATTTTAAAGACTACATCAATGACTAAAAGAACAAATTGTTCATGTAACTGCGGGAATAACTGCTCTTGCGGATCTAGCTGCACATGTACACACTGTGAAACAGAAAATTCTCAAACTTGCTCTTGCAATAAGTAGCAGTAACTATAGACTCTCTTTAAACTCTTAAAGGGAGTCTTTTAGTATCTTTATGTAAAAAATGCTCCACAGCGATCCAACGACTCATCATACACTCGTTCTTAACCTTAAAAAAATCCAAGGAATGATCAGAAAAATAGATACGCTCATCAAAGAAGGTACTTATTGTTGAGATATCGTCCAACAAATTAACGCAACTATTGGACTTTTAAAAAAAATGAATACTACTCTACTTAAGTCCCATCTGATGACCTGTTGAAAAGATAAACTATCCTCTCCAGAAAAGGCAGAGTCTTTTGTTCAGGAATTTTTAAGATTGACTGATTTAAATAAAAAGTAATAAAAAAAAACTTAATCCTGAAAATAGAATCAGATCTCTCTGTTATTTTCTTTAAATAAAAAGCTCAATAAGTAATACTTATTGAGTTTTTGTCTCGCATTTCAGCTTTTCTAAACAATATATTGAGGGGATAATTCCCCTCAATCTACAAAAGTAGAAATCTTACATATTTTCTAAACTTACTTTTTTATTGTAGATATGAAATTATCTACAAAAGTAGAAATCTTACATATTTTCTAAACTCAATGTCTGGAGCCCTCAAGCTCTTATCTACAAAAGTAGAAATCTTACATATTTTCTAAACAAACTCAACAACCATACTATACTCTATAAAAAAATCTACAAAAGTAGAAATCTTACATATTTTCTAAACCTCATAAGAGGTAGGAAGAATACGGGGAATCTACAAAAGTAGAAATCTTACATATTTTCTAAACAATACATTTTTTCGATTTGGTAAAAAAATCTACAAAAGTAGAAATCTTACATATTTTCTAAACCCTTGAGAGCGCGGAAGTAAGAATTGCAGATCTACAAAAGTAGAAATCTTACATATTTTCTAAACTATGAAATATGTTGAACTTGAGCTGAAATCTACAAAAGTAGAAATCTTACATATTTTCTAAACGATCATTTTTATCACTTGCTCAAAACTCATCTACAAAAGTAGAAATCTTACATATTTTCTAAACGACAAATAGTCAAATATAAAGAATTAATCTACAAAAGTAGAAATCTTACATATTTTCTAAACCTTTTATTCCATTTGCATCCATACAACCATCTACAAAAGTAGAAATCTTACATATTTTCTAAACTTCATAAGAGAAAAGCTACTTCTCATCATCTACAAAAGTAGAAATCTTACATATTTTCTAAACGCACGAAAAGGGAGAATCTCCATCCTATCTACAAAAGTAGAAATCTTACATATTTTCTAAACTCGACTTTTGGCTTATTTAAGCGGTTTTGAAGCTATTAATTTTTTCAACCAACAGCTCCAAATCTGCTTAATACCGTCTTACAACTTTATGAAGACGGTAGGTTTACATTTTTTTAGAACCTATGTCGTGATTTTTACTTATAGATTTTTTCTCTATATATATGTTAATGAACAGGTATCTGCAATACTCATCTCCGAAAGTAAGATAATCCAAACTTTCACTGTTTGAATGATAGTGATAATTTCTTTTTTTTCAAGCTTTTTTGTCTTTGCATGCCTTTCAGCTCAAAAGTCGGATTCTGAGATCAAAACATTACTCTCTTTCATCAATGAAAATCCCTACGATTACTGCAAGAGGGACAGAGAGGACAACACCCCAAAAGCCCATTACTACTGCTCCTATTAATGCGGAAACAAGGATTAAGATGGGGTTTATTCACAATTGCTTCTCCATAAGGAATGGAGTAATGACGTTATTTTGTAGCCACTGAACAAGCAGAAAGACGAAACCCACGATAAGCATCCCTCGAAGTCCATTATGAATAAAGGCGAGAATCACGATCGGAATAAAGGCAAAAAGCGGTCCAACATAAGGGACAATATCCAATAGTCCAGAAATCAGAGCGAGAGAAAAGATATTTGGAATTACAATTCCGAAAAGTTTTAAGATTCGAAAAGCGAGATACATAGCCAAAATCACGAAGAGTGAAAGAAGAATCCTTGCCTTTAGTCGGAGGGAAAGCTTTTCATAGATATTATTCAGCTTTTTTCTAGTTTTTTCTTTTTGATCTTGATGAGATGCCTTTACAAAGAGGGAAATAAGGTAATTTTTTTCGATAGAGAAGAAGACAGCGAGCGTAAAGACAATAATCACATTTGTCACCACCCCAAAGAAACCACTAACGAAAGAAAAGAATCTTGATGAGAGATACTTAAGTGAAGTTGCAGAAGTATCAAGTAAGGTATTGAGACCTGAGAGTATTGTTGACTGAAAATCGGTATTTGATCGTGAAAATCCGCTTCGATTTTCTAAAAGATAGGTTTTTAAGAAATCTGGGATTCGTGAAATTTGATTTATCGCTTCTGGTCGTTGGTTTTGCAAGATAAAGTTTTTTAGCTCGATAAGTGTTGTAGAAATTCGTTGTACCAAGAGTGAAATCTGCGAAATCAAGAACGGAATCACAAAGATGATCCCTGATAACACGAAAGCGAGAAGAAAGATATAGGCAATCGTAATCGCTCCTCATCTGGTTTTTAGCCTCTTTTCAAAGGAAAGAATCATTCCTTCCATCGAGATCGCGATAATAATCGCTGTAAAAATAATATAAATCATATCAAGTGACTGCATAAAGAGATAGCCCAGGAATGCAAACAGTCCTCAAATAAGCCAAAATTTGATCATTGATTTTGTTGAGATTCCAGAGAAATTATTATTGCAACGTGAGTTTTCTTTCTTTTGAAGCGGATTTTCTGATACTTCTGTAGGAATTTCAGGCTGAAAGTTTAGCTTTTTTGGTGCGGAGTCTGTTTTTTTCATGATTGTGAAAGGAATGAGGTAAAACTACGTATGAATGTCGTCTGAAGAAGGTGTTGGAGTTGAGCATCATCTTTGCGATCCAGTAGCTCTAGTAGTGGCTTAAGTTTCATCTTATGAAGCGTAATGAAGCATTTAAACCGTTTTCATGAGGCAGGCTGAGATTGGAAAGCTGACTCGAAGGCTTGGATCAGAATTCTTTTTATTTTATGCCTAATGACTGCCCTTTTGGAGACTAGCAAGGGAATATGAAAAGAAATCTGATGAAAGTAGCGGTTTGGATACTGATAACTTCTGGAAGCGAAGCAATTTGAGTGGTAATGAGTGGAGTTTTCATCGCTAAAGTCTCGGTATGGACTGATCAAAATCCTTCAGACAAGGAAGGAGCAACTACTGCCTGCACATTTGCAACAAGAGCTCTCAATTCTTCTTTGGGCAATCCGTTCCAAATTTTCACTCTCCCCTTTTGTGAAGGAGGTAAACCAGCGAGAAAAGTCTGAATCTTCTCTTTGATAAAGGTATCTCTCTGAGCAGGGATCAAATTAAAGATCAGCTGAAGTTCAGGATCCTGCTCCAAAAGCTCAGGAATCGCCTCAACCAAGGTATCAATCCCCTTGGAAATGCCAGTATGCCCGAAGTAAAGCAAATTTCGCTTGCCTATCAGTCAAAACTTATTTTGCACCGCCAAACTCTCTTGCTTACTCACCTTTTCTCTAGACCAGAACTCATAATCTACCCCATTATAAGCAAGGAAGAGCTTTTTGTCTGGGATTCCATAGACTAGTCTCAAAGCATTCAAGGTATATAGCGAAACACAGTGATATTTTGCAAATGGGAGCTGGAAAATCATCCGTTCAAAAAGGCGATAGAGTCGGGCTGTTTTTCGTGATTTGTAGCGGTTTCGTAGCTTTCAAAAAATTTCATGGACTGTGAGCAAAACAGGCTTGCGGGAAAGAATTCCTAACAAACTTGCGGGGATTGCGCCTCAATAGGTGCTTGCATGGATAAGTTGAATGCTCGGGAATGACCTTAAAATCTGCATTCCCCTCCAAATTCCCCAGAAGATGAAATCTAGTCTCCCTTTCCCAGTTCTGATGATCGTCAAGGATCAGGATTGTTCTTTTTGTGGCAAGTCTAGGCTATAGTGCGAGGTTAGGACGATGACTTGGTAGCCCTCTTGCAAGGAACGGCTGATGATCTGCTCAAAAACGGTTTCTACCCCTCCTTTGTGAGGAAGATAATAGTCTATGAGGTAAAGAAGCGTCTTTGGCATCAAGAATAAAAAGAATAAAAAATCTGACTCTAGTGTAAAGGTTTACTCGTGGCTTTCAAGAGGATATTTTGAAAGAAACTCAAGAGAGAAGATTCTCTGAAAAGAGAGAAGATTCTCTGAAAAGATAGAAAAAACCCTCTCAATGAGAGGGGTTCTTCATCACTAAGCATAACTCCTAGTATACTCCAACGTAATATAGCTGAGATTCATCTGCATATTTACAATCCTGATTAGTTGCTGAAGCTGGCGTCAAAGATCCCTTTGTAACGGTCTTACAAAGAAAAAGTTTAGTCACATCATCTATATTCGCTGAACCAATTTTATCTGCTCCCAATACATAGTTTGCACTTCCTGCTCTTTCTACTTTAGCAGCAAAAACGATTGCTCCATTTGCTATTCCATTTCTCTTGAGAATTGCATATCCATACTTTCCTACTAAAGCAGGTCCATCTATAGCTTCATTTGAGATATTCTTTTTTGCTTCCTGAGGAAGAGAAGCGACATTACCGTTTTCTACCAATTTCGCAAGTAAGTGATCAGTAGATCCCTTTGTATCCGTACCGCTTGTTGGATAATTCCCTGTCTCTTGGAGGTAAGCTGCTGTAGCTGTAGCGATCTGCTGGATAGCTGACATTCTCGCAGAATCTCTAGCAGAAGCTTGAGCAGACATAAGTCTTGGAAGGAGAGCCGCAGCCAAGATACCGATGATCACTACTACGATCAGCATTTCCACTAAGGTAAAACCTTTCTTGAAGTTTTTCATTGAATGAATGAATAAAGAATAAAAAAACAAGTAAATTGTTCTGCCTTTTCGTTTATCTATATCATCCCTGTTTTCAAGGAGAACTATAGTAAAGAGGCTGAGTTTTGATTGACATTGATATTTTTATTAGGATAATTTTATTTTATTTTTGGTATTTATCAGATATTTTATTGTCAATTCAGTATTTTATGCGATTGGAATCAACTTTCCTTATTAAACAGGGAGAACACTGGTTTTATTCTGTATTCTCCCTGATTAAAGGATGGTTTAGTCTCCAAACAAAAGTGTTGGGAAATCCATATCAATAAGACCAAAAGCCTTGTCTATGAGTAATGATGAATTTTCTCAGCTCATTATCCTTTCATTTTTACTGAGTCCTTTACAATTTGTGCCATCAGTTCCCGTTCCGAGCACACATGCCCATGAAAAGAGCTGATCTAATCCAATTTTCTCTTTGTAAGGGGCTTTCCATAAATTTCCCAATATTTCCATAACAGTACTTACTCTCTGATCTGTTGGCTTTTTAAAGGTATTGAAAGAGATCAATTTTCCATGGATATAAAGCTTATTACTAATCTTATCTTCACCATTATTTCCAGCAAGGAACAGTCCGTTAATAATGAAGTTTCCCTTTAAGAAGTTAGCCTTTGAATCTTCAGGTCCCTCTGCACCATCTTTGAGATATCCCCAAGGAGTAAAGGTAGCCATATTCCCTGTATTTCCACCCTTAGGAAGGAAAAGGTTTCCTCTGTCAATAAAGAGATTGATTGGTTTTGCGTCCTGAGCGCTCTGATATGCAGTAAGATTTACATTTTGACCTTTAATTATTACGGTTTTCTCTGCAAGATCTTCAGCTGTCACGTCTATTGGAGTGGTATGTCAATTTTTGGTATTATCAATACAGATAACATCAGCATTTGGATCTCTATGTATCCACTCCTCCTTCCACTTTCCTCTACAGAGATTCTCAGCATTTTTACGAACCAAATTTACTACTTGAGAGATTGTGGTATCCGTTCTGAGAAGAAGCGTTGACTCAAGAGAGTTTCCTTCAAAGAATTTTTTCTCTTCATTATTAATTTCATTAGAAAGCCCAATGATTCCCTTAATTCCGAGATTAAAAATCGTCGTTTTTGCACTTCCTGGATCCACATCTAAGAATCATCCTAGTTCAGCAGAACCTCAGAAACCTGTGAGTGTTTTTTTATCGTTGAAGTTAAGTTCTCTTCCATTGTAATCAAATACTTTATTAATACAAACCCCCTCATCTAACAAGCTATTTAATCCGGAGTGGAAGTTATTAACCTTATTAGGATTTCCCATTCCAGATTTTGTAATTTTTGCTCCGATCATTCCTATATGTCCATAGGTATCATAGTAATATCCGAATGGATAAGAGTTATTTAATCTCTGAAGATTACATCTCAGACCACCATTTTCTTGCATGGTATTGTTGGTAGAATTATAGGCAACTCCGGCAGTGAGATGAAGATCTTCTCCATTATAGGTATGTTGGATGTCTCCATAGATTCCATAGGTATCATTTTCTTCTGCATTTTCATTGATTCCTTCACAGTTCGTGTACCATCATCCTACTAATTGTAATTTTGCATAACTTGCATGATCAAAGGCTTGTAAGGCAACCTTAGACTCCTCATCAAGTGGCCACAACCTTTCTCCTCTCTGTGAATTATAGTAGAAACCTCTTACCTTGTAAAAACACTTCTTTTGTTCTAGTCCTCCTGGTCACTGGGTTTCTATAATTACTCCATCCTTTATCTCTTCGTGAGGGAGCCAAAAGAATCCAGCCCCAAAATCATTTCCTCTTACATTTAAGTGAGCAACTGAGAAGTATTCATCCAAAAATTTTGCAGACAACTCAAAATTTAATCCAGTCAATCCCTTACCTCGTAGGCTACTGTTATTTTTATTCCATTTTCCATTTGTTCCTGTAAGTCCTTGCCACTTATCTTTATCTTTATCATCAAGAGCCTTTTCTGACAATATTACTTTTGCTATTGTAGAGTCACTTTGAGGATTTACACCTTTCAATTTTATCTGGAATACTCCTTGATCTATTCCACTTGTCAACTTAATCTTGTAAGTTAGAGCATTCTGACTTACGACTGATATATTGTCTTTTTCTGGAGTTAGAAGTTCATCCTCCTGGATTGGTGTTCCAGCAACGAATTTTCCATTTCAAGGGTTAAACATTTGCCTTGTAAGTCTGACTTCTACTTCTTCTCCAGGATTTAATTTACCTTTTTTGGTGATAACATCTCCAAAGGCATTTACAAAAGTAATTCTTTGTTGCTTTTTATTTGTCGTAGGATTAGGAAGCCACACAGAAATCTTGCTTGTCGCCAACTCCTTGAGCGGTATATAGTTTTTACTAATATCAAGCGTTTTATTTCCTCGTCCTGGCTTTCCCGCGATATAATCCAATGCACCCTCACCAAACAAATTCTCATCAACTTTTAGCACCTTTAAGTTTGGAAGATTATTTAATGATGCTGGCAACTTTCCTGTCAGATGATTATTGCTGAGAATAAGTTCTTCAATCTTCTTTTCTCATCCATTAAGCTGAGGAATTATTCCTGATAATTTTGTATTTTTCAGGTTGATATTCTTGAGTGCTTTGTTTGCAGAAAAGAAATCTGATGGAATAGTTTCATTTAATGGGTTATTTTCGAGGTTGAGAACCTGAAGTTTTTCATTTTTAATTTTTGTTAGTGAAGTTTTAATCTCGTTGTTTCCTAGATTTAATGTCTTCAGATTGATCAGGTTTTTAAAACTGTTTGGTAAAGTTCCTTTAAGTTTATTTCCTGGAAGTTCAAGTCCGACAACCTGCTTTTTGTCTGAGCTACATTCAACTCCTTTTCGACTACAGATATGTTCATTTGCTGCTACACTTTTGTTATTCATTTTGTCCCATCCTTGCTTATTTGCCCAATTTGCACCATCAAGGTTTTTATAAAGATCAACTAGAGCTTCACATTCACTCCTTGAAATACCATACTTTCCTTCAAAACGACGACAGTAGCTGTCTTCATATGTGAAAGTAATCTCTTTCGCCCAGTTTTCTGGCGGGATTGTTCCAGTAAAGGAAATCGTTTTTGCTTGTTCTCATGTAATTGTAGCGCCAGCGATTTGCTTTCCTGTATAAGTATAAGACTGGTTTAGCTGAAAATCCAATCCTCCGTATTTCCTCAAAACTTCTTGTGGAGCAATTGTTTCTCCAAAGCTATTTTTGTACTGGATAGTTACCATTTTGGTGGTAGCAGCGATTTTATATTCTTCAAGAATCTCACTAGTGCTCAGTGCATAGTTCCATATCTTCAATTCATCGAGTTGAAAATCACTCTGACTGCCATTTGTAGCACCAATGTTTGCGTTAAATAGACCAAGTTCTGGATAAGAATCTAGAGGCACCGTTCCTTTAGCTTGACCATTAACAAAGCAAGTAAACTGATGATTGATCATATCAAAAACTCCTGCAATATGGCTCCACTGCCCTTCTTGTATAGGAGAGCTTCCACATTGAGCCTCTTTTGTCTTGTGACCATTTCAGATAAAGAACTTAATATATCCCTGGTTATCGATTCAGAATTTAAACCCTTTATTATTTCCACTTGTAGCATCAATTGTATAGTTCTGAGTTATATTTTCTGGTTTAATCCAATGAGATACGGTAAAATACCTTCCTCCATCTAGCTCGATAGGCTTTGCTGTAGTAATTTTCGCTGTTCCTGCAAACTTAGCCGATTTACCTAATCTTCCTTCTAAAGCAGTCGCTCCATGATTAGCTCCCACCTGTCAATCTCAGGTAGCATTACGACCAAGATTTTGTGGATCATCAAAAGAATAATTCAGAATTTGTTTCTTCTTGTAGGTTGTGAAAAGCTGAATATTGGCATTATTTGCGACATTTACTGTTGTTGGCTCTTTGAAAATAAGCCCTTTTACTTTTCCTCCCAAATCTCAGAGTCCTACGTTGTTTGCAAAAGCCTCCTCTCAAATTTTATTGAGTGAATCTGGGAAATATATAGATGTAAGTTTATTATTCTGAAAAACGTGCGCATTAATCTCTTCAATAGTATTTGCTCCAGTCATATTTACAGAAGCAAGCTTATTATTCCTAAAACTTGATTCTCCAATGATTTTTAGAGAGGCTGGCAACACTAGCGAAGTAAGTTGGTTATCACGAAAAGCTTCGTTTCCGATTATCGCAACTCCGCTGAGAAGTTTTACTGAAGTGAGCGAGTTATTACGGAAAGCTGAATTTCCGATACGACTTATTCCAGCAGGAATTTCTACTTCTGTAAGTTGATTTCCAGAGAAAGCGTTATCATCAATTCTTTTCGTTCCACTTGGTAGGAGAATTGTTGTTAGCTTATTATCTTGGAATGCACCAGTTCCAATTCCATTTAGAGCATTTGGAAGCTTTACTGATACTAATTTATTAGCTCTAAAAGCTCCTTCTCAGATGAATTTTACAGCATCAGGAATTTTAACTCATGAGAGATTATTTCCTTGGAATGCCTCCCCTTCGAGAGTAGTCAATCCAGTTGGAAGCTGAATTTCTGCTAGTTTATTGCCTCTAAAAGCAGCTTGTCCAATATGGGTTATCGTATTTGGGAAAATCACGCTCTTGATTTCTTTTTCTTTGAAGGCATTGGATCAGATTTTTTTTACTGCTTGTCCATTGATTGTAGCTGGGATTGTTACGTTTTCATTACATCCAGCTCATCCCTTATTATAGTTTGTAATTGTTCCTGCATTAAAGGTGAAACAGCTCTCTGGTGTTACTAATTGGATCAAATGGATATTAGCATCCGATGCTCTTGATATCTCACTTGGATCTTTCAGAGTATATCCTACTACCTGATTTCCAAATTCAGATTTTCATGGATTATAAGCGAAAGCTCTTTCTTCGATAGAAAGAAGACTATTAGGGAGGGAAATCTCTGTTAGCCTATTCATAATAAAGGCCTCCCTGTTAATCTTGATAAGCGTTTCTGGGAATACAACGGACTCCACTCCCCTTTCCATAAAGGCTCATTGTCCAATTTCTTTTACGGTAATACCATTGATAATGGATGGGATGAATACATCTCTACCACATACCCCTCATCAGTTATAACTCGTAATAACTCCTCCTGAGGATATCTGAAAACAAGCATCAGGAGTTGCTTCTGTCCTTCTTACAATTTTAATCTTTGCAGTCGGATCAACTTGGACTTTTGAAGGATCTCCATTAATCACCCCTTTTACCATGTTTCCTCAAAGATCAGGACTTCATAGATTAGACATAAAGGCATTTTGCTCAATATTTCTGAGGGAAAGTGGAACATTTACCACGGTTAATTTATTGTTTTCAAAAGCACTTTTACCAATATTTGTTAGTCCAAATGGAAGATAAAGTGAAGTTAACTGATTATTAGCAAATACTCATTCTTCTAGGGTTTTTAGTCCTTCTGGGATATAGAGTGAGGTGATTCTGTTAGATTTAAAGGCATTTGCTCAAATTTTCTCAATATAGTAAGGGAGTTGCAATGAAGCAATCTCATTTGATGTAAAGGCATATTTCCCAATCTCCTTCACTGAGTAAGGCAAGATGAGTCAGGTTAAATACCCCCCCCAAAAAGCATAATCCCCAATCTTTGTGACGGAATTTGGGATTGTTACTGAGGCCAGAGGATTATAATAAAAAGCATAATCTCCGATCTTTGTTACTGAATTTGGGATTGTTACTGAGGTCAATTGATTTTCACCGAAAACATGATTTTGAAGTTCTGGAACAGAATTTCAGATTATTACAGATGTTAGTTTATTTCTCCTGAATGCAGCATATTCAAGGCTCGTTACAGAATCAGGAATTATTATTGATGTTAGCTTATTATCCATAAAAACAGAACCACTCAGGTTAGTTACAGAGTTTGGAATTATTACCGAGGTCAGTTGATTATCTGAAAAAGCGCCGAATCGAATACTCGTTACAGAATTAGGAATTGTAAGTCAAGTAAGTCCTTTATATCTAAAAGCATTCGTTCAAATAGATTTTACCGGATGTGCACCAATAGTAGGAGGAATAATTACGTCGGCTCAACATTCAGGATAAACTCCTTTTTTATAATTCGTAATTTGCCCCTTGGCAGTATCAAAGTCAAAACATTTCGCTGGAGTTCAAGGTGATACAAGCTGGATATTTGCGTCTGATGCGACACTAACTGCACTTGGATCACCAGTGAAAATTCCCTTTACTTTTCATCCTAAGGACGAAAGTCCAGGATTACTATTGAAAGCATACGTGCTAATTGTCGTAACAGAAGTTGGGATCTCTACTGATGTGAGTTTATTTGCTTGGAATGCTCGAAGATTAATATGTGTTAGTGTATCTGGTAGTGTAAGTGATGAAATTTGGTTATTTCTGAAGGCATGATTAGCAATTACTTTTACTCCACTTGGAATAACGACAGAACTTAGCTTATTTGTCATAAAAGCTCAATCGCTAATTGTAGTAAGTGAATCTGATATTTCAACAGAAGCTATTTGATTATCCCTGAAGGCACTATTTCCAATACTTGTTACAGAATTTGGGATTACTACTCATGTCAACTGATTATACTGAAATGCAGTTACTCCAATGCTTGTTACAGAATTTGGGATTACTACGGAAGTAATGCTCTTGTTTTTAAATGCATTATCTCCAATAGCAGTTACAGACTTTCCTCCGATCGTAGAAGGAATAGTTACATTATTTCCACATTCAGAATATGCTCCCTTCTTGTAGTTGGTGATCGTTCCAGAATTAAAGTCGAAGCAACGCTCTCCCTCTGGAAGCGGAGGGGTCACTGCACTCTCTGTCGTTGACGCACATCTTGTAGATATCCTTTCTCCTCCTGCACTTCAACATTTTCAACTCGATAAAGTACAGTTATGGCTATATGCTGTAGAATTTGTGACTCTATAATGGTTTGATTTTTGCTGTCCTGCCGCTGTTGCGTTAAATCTAACCAATTTTCCTCTATTAACATAAGCATGATTCCATTCTCTTAACCCTCCGTAGTTCTCCATCATTTTTCGAGAAAATCCAGCAATATTCAGACTACGACAGTGATTAAAAGCTGCTGTTTGATCTCCTGCTATTTCAGTATTAGCTGGAGCAATAAGGATTTTTGTCGATCCCGAAGGAATCCCAAGAATACAGTATTGACTTGGATTACTAAGCCCAGGAGTATTACATCCCTGAGGATAACGGCTATCATTACTATCTATGATATAGTAAGGTGCTGTCTGCGCTGATACCGTGCTAAAAATTCCACTTCCTAGCAAGAATGCTACTCCTGCTCCCAAGAGTGTTTTTCCTAACCATGAAGTCATAAAAATTCTTCCTTTTCTTTTGTTCATCAGTATCGTATAACAAATAAAAAGTATACTAGCTCAAGTATAGTTTCAATACTCCCAAAAGTAAAAAGAATCAGAATTAAACTTTTTTTTCTTGTTGTTATTTGTTTTTTGTTTTTGTTTTTATATTGACTCTTTTGCATTTTATAATTTCTTATAAAAAATAATAGCGGGAGATTTTCTCTTGCATTCCCGTTGCCCATTCCTATATATAAGAGAGTTTTTATCTTTTTGTTTGTTATGGATCCCCGATCGTTAGTCCTCTTTGGTATTTTGATTTTATTATCAATGTTTTTCTCTGGGAGTGAGACTGCGTTTACTGCGGTTCCTCTTCACAAGGTCAATGCACTTAGGAAACAAAAAAAAAGCTGATCCAAAGTCCTTTCTCAGCTCAAACGTCAGCCGGAGAGAATGCTGATTGCGATTCTCATTGGGAATAACATTGTAAACATTGCGGCTGCCTCACTAGCGACACTTATTTCACTTCAGATTGCAACGGTAATCAATTATGATCAAGGCACGATTATCACAATTTCTACGATTATCGTTACGATATTGGTCTTGCTCTTTGGTGAAATTTTTCCCAAGACCTTTGCAACGAGTCACGCGGAAAGGATTGCTCTTGGTATTGCTCCGATTTATCTGGTACTGATTAAAGTGTTTTTTCCGATTATCGTCGTTATTGAGCGGATGATGAAAGGGCTTACCAAAAAGGCGAAGAAAAATGCTGTCAGTGAATCAGATTTAGAAGCTTTTATTGAGCTTTCTAAACAAGCGGGGATGTTTGATAACAAGCAGGATCAGAAGATCAAGAAGCTTTTGGCGCTTGATGATTTGACTGCTGAGGAGATTATGACGCCGAGGATCAAGATCAAGGCGATTAATGACGATCATACTTTGGAAGAGGCGATTCAGGTTTTGACGGAGTATCATTATTCTCGTATCCCTGTTTATCATGGGACGATTGATACTATTGATAGGGTGATCACGCTAAAAGAACTCCTTAGAATGACCAAAAAATATCAGTGATCAACACTCCTTTCAGAGCTTCAGATTTCTCCTATTATCAAGATTCCTCGTAGTCAACCAATAGATAGTTTGCTCGAGAAATTTCAGAAAACTCACAAGCATATTGCGGTAGTGATGGATGAGTATTGAGGAGTTGACGGAGTTGTTAGTCTAGAAGATATTATTGAAGAGGTTTTTGGAGAGATCCAAGACGAGACGGATGAAGAGCTTCCACTTATTCAAAAAAATGAAGACTGATCTTGATATCTTTGTCAGTCTTATACGAGGATGGATGAATTACTCACAGAGCTCGGCATTCATTTTGAAGATCTCAATTTAGATCATGAATTTGAAGCGGAAACCTTAAGCTATTTCATCACCAGTCATCTCGAGAGATACCCTCTTGCCTGAGAAGAAATCGTGCTTCCTATCAATACCCTCGAGGAGAAAGAAGAAAAAAAATCACTCCATCTTAAAGTGCTTCGAATGAAAAAGAGCATTATCGGTGAAATTAGTGTAGCAGTAAGATAAACACGCGGACGTCCGTATCTTTTGCTAGGAACGTCCGTATCTTTTGCTGGGAACGTCCGTATCTTTTGCTGGGAACGTCCGTATCTTTTGCTGGGAACGTCCGTATCTTTTGCTAGGAACGTCCGTATCTTTTGCTGGGAACGTCCGTATCTTCCTAGCAAAAGATACGGACGTTCCCAGCAAAAGATACGGACGTTCCCAGCAAAAGATACGGACGTTCCTAGCAAAAGATACGGACGTTCCTAGCAAAAGATACGGACGTTCCCGATAAAACATGCGAACGTTTTTGGGGGAAAGATTGGATTTCGTATTTTATCAGGATAAAAAGTAGGTTTATAGAGTATTACGAAACTAAGCCAGTAACTTCACTCTAATAGCTTGCATCCCTCACTTTCTCTTCTCTTTCGGTGAATCTCATAATTTCTTTCTCAAAATGGAGCTCAACCTCCCCTACCGCTCAGTTTCTGTTTTTTCTGATGCAGACGTCGGTTGCACCTTTCTTATCAGTATCAGGATCGTAGTAGTCTTCTCTATGCAGCATCAAAACCGCATCGGCATCTTGCTCAATCGCTCCTGATTCTCTCAGGTCGGAAAGTTGTGGCTTCTTGTCTACCCTCTGCTCTACCGCACGAGAAAGTTGAGAAAGTGCGATAATCGGCACTTCAAGCTCCCTTGCAAGCTCTTTTAGCCCTCTTGAGATCTCGGAAATCTCTTGGACTCTATTCCCAACAAAGACTGTTCCATGCATCAGTTGGAGGTAATCAATGATCACAATATCCAGTCAGCCTTTTTCTATTTTTAATTTTCTGAGTTTAGATTTGAGCTCAGGAACGGTCAAAGCCCCCTTATCATCAATATAAATCTGAGCTTCTCCAAGGATCTCCATCGCCTCCCCCATTCTCGCAAAATCCTCATTATCTAGGTCCCCTTTGGTGATTTTATACATCGGAACGCTAGAAACCTCCGACATAATCCTATCAACGATAGATTCTGAGCTCATTTCCAGGGAAATCATCACGGCAGATTTTTTCTGTTTGAGAGCAATATCGGTCAGCACATTGAGGGCAAAAGAGGTCTTACCCATAGACGGTCTTGCTGCAAGAATAATCAGTTCTCAGGGCTTGAATCCCGTCAGCATCCCATCAATCCCCTTATACCCCGACATCACCTTCTTTTCGTTGAATTTCTCAGGGTTGTCCACGATTTCCATATACTCCTCTATTCTCCCTTTCAGAATATCTCCAATAGAAATAATGCTGTCTCCGACTTGGTTTTGCGTAAGGTCAAAGATTTTCTTTTCAATAGCATCAAAAATCTGAATCGTGTCTTTTTGTTCGTAGACTTCACCTGAGATTCCCTGACAAACTTTCAAAACTTGCCTCAAAAGCGACTTTTCTCTCACGATTTTGCTATATTCTCAGCAAGGCTGAGTAGAGAAAAGGAAGGTAGAAAGCTCGTAAAGGTAGTCCGTTCCTCCGATCACATCCAGATTTCCATTTTTTGAAAGCTGATCAGAAACGGTAACGACATCTATCGTCTTTCTTGCAGTCCAGAGCTGCTGAATCGCCTCGTAAATAAATCCATGCTCCTTTTGATAGAAATCTTTGTAGCCCAGTTTATCACTATCATAAATCCACATCGTTTCATTATCTAGCAAAACTCAGCTGATTACGCCCTTTTCTGCTTCAATATTATGAGGAGGGAGATTGATATTTTCTATAGCCATGGATGGAGAAAGAGGAAAAGAATAAAATTAGAGAACTAACCAGTCATTGAGAGTTATAGACGAAGCAATCCAGTTTTATTGCTAATGGATCGCCACACTTCGTTCGCGATGACTGATTTCCGCATTCCGTCATTGCGAGCGAAACGAAGTGGAGCGTGGCAATCCAGATGATAATCCAATCCATTTCCAGGACAGGATCTCCCTCGCAACAAGTCGGAATCGTGATGACAAAAATCCATACACGAAGATAATAAAAAATTCCGAACCAAATGCAAGAGAAAATTCAGTTCAGAATTGGTTTTTCTTTTAACTTTTTCACAAGTTTTTAACAGAGTTATTTAATTCAAAAAGCTTTCTTCAGTTTCTTTATCGCCCAATCGTAATGGCTTGAGGTCGCAGAAATACAGTAAGACCCCAAACTTGTTGTCCCAGTCCAAGAAAAATAAGCTTTTGTAAAGAGTTCCTGATCTGAAAAGCTATCTATGAGCTTCATTACCTCAGTATGACTCTTGTTTAGAAACTGTTTTGTGGTTTGAAGCGAAGTTGTTTGATGCTTTTTTCGCAATGCTCTATTCATTTCTCCATATGTTTTCCAAGTATAAGGAGCGGGGAGAAATGAGGTCTTGACTCCTTTTTGGTTTTCTCCAATCCAATGAAGCAAGAGTTGATGCCATTCATAAAGATGGATAAGAACATCTCTCAGATTTTTATCTCTTTGCCAATGTGCTTCCTTCTGGCGAGCTAAAAACCCTTCATCAAAAGAAAAGTCTGACACTTGGGCTTGTTCTGAAAGAGAAGTTATCAGATTTTGAAGCTTCTGAAACTGCATTTCTGCGGCTTGAAGTAAGTCTGATTTGTTTGTTGGTCTTGGCATTCTTTTCAAGAACATACAAGATAAAATGCTTAATTCCACAAATGCTTTGGATAGATTCCCTGCTCAATAAGCTGATCAATATTACTACTTGTTGCCGCTTTGTCTTCCTGATACGTCACTCCAAACCATTTTGCAGTAGTCGGCAAAACAGGAACGGACTCTCCTTCCTCGTTAATTAGTCTCGTGATGACTTCCGGCATATAAAATTCAGTTTTTGGTTCGTTGAGATTCTTTTCTAAAAAATCAATGAAGTATTTTTCGTAAAATTCAAGACTTGCTTTGGTAAATCCCATCATATTCATAGAACAAAGATCCTGAGGATTGAGTTCATAAGTCGTTCCATCAGGGTTTTCAGCGAGGATTTTTCCTTCTGATTTGAAGATTTTTTTGGTTTCGTGAATGGCTTTGAGATTATTATTTTCATCAAGTTCGCAGATCCCCCTAGATACTGTTCCAAAGTCTGAAAGTACTTCTGAAAGTTGGTAGCCGACGATACAGTTTTGATTATGAGTCTTCAAAAAGTTAGCCAAGACCTGATAACTCTCCCTACCATAGAAATCATCAGCATTGATCACAGCAAAAGGTCCATCAATCAAGTCTTTAGCAGCGAGGACGGCATGCCCGGTTCCCCAAGGTTTTTCTCTCGTAGATGGGCAGATGAAGCCTGCTGGAAGTTCATGCATATCTTGGAAGGCGTATCAGACCTCCATTTCCTGTTCAATCCTTGAACCGATTCTGCTTTTAAATTCTATTTCAAAGTCTTTTCTGATTACAAAAACGACCTTTTCAAATCCAGCTTTTTTCGCATCAAAAACGGAGTATTCCATCAAGGCATGCTCCCCTGCTCCAAAGGTATCAAGCTGTTTCAAGCCTCCATAACGGCTTCCCATTCCTGCGGCGAGGATTACGAGGGTTAGTTTCATTACTTATTTTTTAAGGATAAAATGAATACAAGCACTATAAAAGCCACAATAGCAAGCCCAACATTCAAAAACACCTAATACCTAAAGAGAAAAGCTAAAAAAACTGATTAGATTTCCCTGGTAAACTCTCTGATTTTTGCTTTTGCTTTTTCGTTATTTTGTTCGTATTCTAGGACGTTAAAGTAGTATTTCTTGGCATTATCCAGGTCTCCAACTTGCTCATAGAGTTCTGCGATAGTTAAAAGGTATTTTGGATTCGTTGGTCTGAGTTTAATTACTTTTTCCATATAGGCGAGTGCTTCTGCATTTCTATCGGTATTATAATAGATTTCTACCATTGTGAGGTAGTATTTTGGATTATCGTTCTTAAGGTTAATCGCCTTTTCTACCAACAGTTCAGAAGTCTGAATATCTCAGGTCACAAAATAGATTTCGGCAATTTGCCAAATCACTTTGTGGTCAGTAGGATCCTGTTCAGAGATTTTCTTGAGAAGCGGAAGTGCTTTTTTGTAATTTCCTAGCGTAAAATAGAGGTCTGCAAGCGGTTTTAGAACCTTAAGCGTCGAATCATCAATTGCTGTAGCTTCAATAAGTTTTTTTTCATATTGTTCTACTTTTCACTCGTTTTTTAAGAGTTCTGCCTCGAGGAGAAGCGTATCAAGCCTTTTTTTCTCCTTTTCTGAAGTCTGTCCCTTGAGTTCTCCTTCTTGATTTCCAACTGGAGAAGAGTGATTTTCTTCTCACTCTAACGGAGCAACAAGATTTTCGTTATCAAGCTTTTTGTCTTCGATCTCTTGTTCCTGGGCGTGAAATGGTCCGTATTCATTACTGGCAATAAGCTCGTCCTTCTGCTGTTTGAGTTCAGATTTTATATGTTTAAGCTCTACATTTTCTTTGATAAGATCCGCCTTTTTATTGAGGAATCAGAAAAGTTTCTTCCCAATTCGATAAAGAAGAACTCCCGTAGGAACTACAATAAGGGCAACAGTAAGCCCTATCCAGATATAAATGAGTATTTTAGATCACATCGCTATTAGAATAATGGAATAAAGAGTATTTCTTGATCACTACGAACATGATTCAGATTTTTCTTTTGTTTTTTTGATAAGTGAGAGCGGGACTCTCAAAACTCCTCAGTCCTCCGTCTTTATCACCACCTCTCTGGTAATAATATTGTAGGAACTTACGATTCCATTCTGTTTTGAGTTTGGACATTGGATTTTTTCTCCTCTGTGAGGGAAGTCCTTCCCTTCTTGGGCATAAATTTCTAGCTCATAAAGCAGACTACACTTGAGTTTTCAGCATCTTCCTTTTAACCTTTCGGTGTCTCTCCCCTCAAGTCCTTGCATGGCGATGCTTTCCATCTCAACGCTAGGTAAAAGTCCTAGTCCCTGACAAGCGATTTCCATACCGCAGTCGGAACCTACAGCATAAGCCTTTGCATTAGGATCGAGCCTCATCATATCTCTCGCTCCCACCTGGAAAAGAAAGATATTTTTTCAGATTTCTTGCCTAAAGTCTCTGACAAAATCACTAAAAACGTATCTCTCCTCAGAATAAAAATAGAAATAAAGCTGATCCATAATAGGGTTATATCTGGAGGAGATTGGCTTGGATCAAGGGAAACTTTGTTTAAATTTTTTTTTGAAAAGCGGGAAAACCTTGAGAGCAAATTCCTGCTGTTTTATAAACTCCTCACATTCTTGAGAGGTCAGTTTTTTTACGAATTGTCCAGTTCTTTTTTCACTGATTTTTAGTGGATAGAGAATAACCATCCCAATAGATTCCTTTTCTGTTCCTTTTTCCTCTACGAGATAAGATATTTTGTCTCCATGCGTGAAAGTTTGATAGCTCTCATCAGGAATATTGAGAATAGTGAAGTGTTTATTCATAAAGTAATCAAGCACTACGATAGTTTGCATAACAATGGGTAAGAGTAAAGTTTTTTTAAGATTGTCAAGTGCCTCTCTGAAAATATGATTTTTATAAGGAAATGCAAGACTAAGTAGAAAATCCTGTTTTCACGATAGAAAATAAGAATTATTTATAAGGCATTTCTTAATTTCCTAGCTAACTACTAGGGAAAAGCATTTTTAAGTTTTGCAATCAGACTTCACTTACTTTTCTTTCCTTTCAGAGGATATAAACTGTGATGTCGCCTTCGTGGTGCTTAAGGATATTCATCAGGCTTTGAATTTTTTCCATATTCTCAGGTAAGGTCTCCAAAGTAAAGGTTTTTTCTTCTAGCTCTTCGTCTACCTCTTCATCGCCGTGTTGATCGACCTGTTCATCAGCAAATACTCATGACTCTTCCTCTATAAATTCTTCTCCTGGAAGCTGTTCTTCCTCGTGTTTTTGGGAATTTTCATTCGGTTCTTCGAGCTCCACTTGGCTTTTTTCTGCTTTTATTTTTTCTATTTCTTTATTTTTTTCATCTCCGTATCTTTCTTTTTTGGCTCTTGCAACTGTCCATTCAGGATCATATTTCCCTCATGCGAGCCTTATTAAGTTTTCATAATCTGTTTTTACTAACTTATCCAAGTATATCCTTCAGTTTTTTTTGCTTCCATGCAAGATAATCAAATCAAACTTATGGAAATCTAACGCATCTTTTGTGAAAAATTCCCAACTCGTCGAAATATCTTCAATCTTGATAAAAAAACCCTTCTTTTTTGCCCTTTGGACCTCCTTAATATACCCTACAATAATGAATTTAGGGAAATTCTCTTGAGCAAGCACTTGATTGAGGAAGGAAAAGTTTTTGATATAGAGATAGAGCCCATCAAGTGGATTTCACGAAACAAAGGCCTTCAAGACTTCCTGCTCCATCATGAGCCTTTCCATGAGATTTGAGGTTTTTACTTGTTTGAGCTGAATACTCGTATCAACTCCAAGTCCTCAAAACAGTCAAAAATCTGCATTTGGAATCGTTTTACTTCGATCGATAAGGGTTTGCGTATTCTCGAGCAATACATTCCTGTCTCCAAAGCTGTCCAAGGCTCCACACTTAATAAGTCCTTCCAAAGATTTCTTATTGATAATGGTCGAACATCTCTTGAGAAAGTCCTCCAAACTCTGGAAGCTTCAGTTTTTTTGCCTTTCTTGCTGGATTACCTCTCCAACATCTTCACCGATTCCCTTTACACTAAAAAAACCAAGTCTGATCGCATCTCCGATCGCTGCTACGTGGTTAAATGAGGCATTCACATCAGGAGAAAGAACAGAGATTCCTTTGTTTTGGATTTCTGAGATATAAAAACTCTGGGTATCAGTGTCTTCTTCTACAGAACGAATCAATGCCGCAGAAAATTCTACTGGGAAATGAGCCTTTAGATACGCTGTTTGGTAAGCAATCATTGCATAACAAACTGAATGGGATTTATTGAACGAATACGATGCCGCAGGTTCAATCATTTTTTCGTAAATATAGGTCGTTGTTTCCGGTTTATATGCCCTAAAGCTACCACCTCTGGTGATAAACTCTTTCTTTAATTTCTCAATTACTTCTTTTTTCTTTTTTCAGACTCCTCTTCTGAGCATATCGGCTTCTCCCAGTGAAAATCCAGCCATCGCTTGCACAAGAAACATAAGCTGCTCCTGATAAATAGCAATTCCGTACGTTGATCCCATAATCGGAGAGAGATCTTCAATCAGCTTTTGATTTTCCTGTTCTGCTACTTCTGCACTATACTTTTTTGTCAGGTCTGCTCTAAGTTCTTCTGTCATGTATTCCACCGGCTCTCTCCCATTTTTTCTCTCAATATATCTCGGAATAAACTCCATAGGTCATGGACGATAAAGTGCATTCATAGCGACCAGATTATCAATTGAGTTTGGTTCCAGCTGGATCAAGAATTTTCTCATTCCCTGGGATTCAAATTGGAAAATTCATGTTGTATCTCCAAGTTTAAAAACACTGTCGTAAGTAAAGGCATCTTGAATATTAGGCTGAAATGAAGTCGTTTGCAGGAAATGTTCAAAAATCTCCGGTAAAGGCTCGCCGGCTTTGTCATGTCTAGCTTTTATAATTTTGATACAGGTTTTGATGATAGAGAGATTTCTTAAACCGAGAAAATCCATCTTCAAGAGTCAGATTTGTTCTAATGTGGGTCCATCATACTGACTCACAACGGTATGATCTGTTTCTTTTGCAAATTGGGCAGGAGTATAGGTAGAAACAGGCTCTGGAGCGATGATAATTCCACAGGCATGAACCCCCAGCTGTCTCAAATTTCCCTCGAGATTAGATGCCAAGTCAAAGGCATATTTTACCTTTTCGTTTGTGGCGATATAATTGTGTATTTCCTCGTATTCTTCTGCATTTGAGATAATTTCTTTGAGTGAGCTTTTTTCTGGGATAATATTAGAAATCTGATTCGCCATCTCAAAAGGAACTCCAACCGCCCTTGCCGAGTCTTTGAATGCCGCTTTGGTTGCCATTTTCATAAAGGTTCCTATTGCACAGACCTTTTCTTGACCATATTTTTCTGTACAATAATCAATCACTCTTTGTCTCTGGGTATCCTCAAAATCAATATCAAAATCGGGCATAGAAATTCTCGCAGGATTGAGGAATCTTTCAAAGAGAAGGTCAAATGGAAGAGGATCAATATCGGTGATCTCAATCGCCCAAGCTAGCAAAGATCCAGCTCCAGAACCTCTTCCTGGACCTACGACGATCATCTGTTTTTTTGCCCATCTTACATAATCTGAGACGATCAAAAAGTAGGAATTAAATCCCATTTCCCTGATAACCTTGAGCTCATATTCAAGTCTCTCAATATAATTTGCAAGCTTATATTTTGAATTTTCGAAAGGGATTATATCTCCCTGTTCTTGGAGAATCTGCTTTTTAAACTCGGTATAGGTTTCATAGGTAAATTTTTTTAGATCTTCTGGTGAGGTCTCAGGAACTGATTTTTCCAAGACTCCCCTTCCTGTAGTTTTCACCAATTTTACTAGAACATCGGTATCTCGATTCAGTTTTAATCTAGTTTTTAGTCCTTTGTAAGCGTGATAACGAAGCTCAAACTCATGAATGTCCATAGTCATTATGCAAGAAGGGTTGAGAAGGGTAAATTTTCTTCTTTTAGTGTAGGGTTTTGGTAACGAAAATCAACTTAAAAAAGAAAAAACTCCACACTATTGTGGAGTAATTTCTCAGTTTTAAAGGCTTTTTTATGGATTTGTTGCTGAGCCTGTAGTCTTAGCACCGACAGTTCCAATAAGCCAGAAGATAATACTTACCATGAACCATGCAAGAGCGATGAAGGCAAGTCCGATTGCTGCCTGTTTGAGAATCTTGAATCCAGCCTTGTATTTTCCATCATCTCCAGCTGCAGTCACCATCTGGAAACCTCCCCAAAGGAGAAGTACCAAGGTAATAAGCGCGAGGATTCCTAGTGTCCAGTTGATAAAGTTTTTGATAATCTGGATTAATCCTGCTTGTTGATCTTGTCCTGTTCCTGGAGCATTGATTCCCGTTGTTTTGTCTGGGTTGATTCCAAACTGATCATTAAGCACTTGCACATTCCCTTGGCTTAGACCGTTATTTTGAGCAAGAGCTATACCACTCGCTCCTCCAAAGAACGCTAGTCCCATCATCATAAATGCCAATAATTTTGTTTTCATCTTCTTGTGTAAAAGGATTAAAAGTAGGGTGCGTAAGTCTCTGCCCCTCTTGATACTTTAATTTTAACCAAATCGTTTCATTTTGCAAATTTTTTATGACTTTTTGGAGGGAAAAGTTGCTTATCTGAGAAAAGGAATGCGTAATCTCGTATTCTACCTTGAAAAAAAGGAGTGAATCCCTACACTTTCATCCAGCTTTTTTATTTTTTAGGCGAGGGATGCAAAAGATTCTGAAGGGACTTTCTATTTATAAAATCTGATATGGAGGGATCGGCATCGCTCAGCTCCCTGATGGAAAAAAGGTTCTGATCAAAGGTGGAGCTCTGCCATGAAGCATCGTCGATGTTCGTATCGTAAAAGCAAAAAAAGACTATGTAGAAGCACATCTTCTTGAGATTAAAAAGCAAGATCCCCAAATCCTTACAGGAACTCCCCTTTGCCAGCATTTTTTTTCTTCTTTTCTACAAGAGCAAAACCCTGATCTTCAGCCCAATGAAAAAGGATGTTGAGGCTGTAAGTGGCAAGTAATGAACTATGAATCTCAACTTCAGCTCAAACACGATATTATCAAGGATGCTTTTTGAAAACTCGTAAAAAAACAAGAAATCTGATTTCCTCCTGTGATTGGATCTCCACTTCAAAAGGGCTACAGAAACAAAATTGAGTTTAGCTTTGGAGTGTATAAGCAGCTGGACGACTCTTTTAGAAAAGCGAAGAAAAGCTGAAATTCTGAAGAATCATTGCTTGCCCAAGGACTACAAAAGTATGCTATTGATGCGGGTTTTAATCTCGGTTTTCACAAGCAAGGAGAGTTTAGTAAAATTGTGGATATTTCCTCTTGTGGGCTTATTAGCGAGAAAGCGAATCAGGTTTTTGCCTCTTTGAAAGCTCTTTGCAAAGACTCCGGATTGCCTGTTTATGATCAAAAAACTCATCAGTGATTTTTTCGTCATTTAGTGATCAGAGAGTGAGTAAACACGCATCAAATACTCATCAACCTCTCTGTCGCCAATCAAAACCTTGCTGCTGAACAGGGAGAAATTCAGCTTTGGGAGCAATTTTTAGAAAAATTAAAATCTGACACTTTTCTTCAGGAACAGGTTTCTACCTTTGTTGTGAGTTATAATAATGGACTCGCTGATATTGTGCGTAATGCAGAAACCGAGATTGTCCCACTTTGGGGAGAAGGTGTGATTTATGAAATCTTGGATTTTTCTCAGTTTTCTAACTCTGAATCCGCGAATCAAGAACAAACATCATTAAAATTCAGAGTTTCTCCCTCTTCGTTTTTTCAAACCAATACGCTTGGTGCTCAAAAACTCTTTTGAACTGGGCTCAAACTCTTGGAGACTATTGAGGGAAACATCTTGGACCTGTATTGTGGTGCGGGAAGTATTGGGCTCTCTTTCCTTAAAATGGGAATTGGAAAGGAGCTTATTTGAGTTGAGATCGTAGAGGCGGCAATCGTTGACGCTGAATATAATGCTAGGATTAATGGTTTACAAGAGAAATCTTTTTTTGTAGCATCTCCTGCCGAGAAAATGCTCACCAAATTTCCAGAGCTTAAGGAAAAAATTCAGAATGTTTGACTTGTGATTATAGATCCTCCTCGTGAAGGATTACACCCGAATGTCATCAAGTATTTGGCAGACCTAAAAAAACAATATGCATTTAAGTTGCTGTATATTTCTTGCAATCCAATCACAATGGCAAGAGATATTGAACTACTAGTTGACGAAGGTTTTACTTTCAAGCAGGTGCAGGGACTCGACATGTTCCCTCACACACATCATGTTGAATGTATTTCTGTGTTGAGTTAACCTGAGCTTTTTCGAGCACTTCCGAAGAGAAAAATATTTTTTGTTTTTTATTTAGCTGATTCTCTTTTGTTTAATCTTTTTTGAACTCAAAGAGCAGCCCTTGTAATCTTATAACTTTAGCTATAAGCTTTATATAATTTCAATTTCATTGATATCCAGTCAAGCCAACCCTGTCAATTCCTTTAGGGAGAACCTTTGCTTTTTTGGCTTAATTCTTCGATCTTTTCGTGAGGCGCAAAGGAGATAAGCGTTCTGATTTATATAATTCTTCATATGCATTATTGATACAAATAACTTCTCAATTTTCCGCTGTTGCTATATAGATTCTCTCCTTCCAGTATCCACTACTTGTTTTTACTTCAATAATATCTCCTATTCTCGGCTGATCTAATCCAAGTATGCAAAACTCATCAAAATAAAAAAGATCATACCCCTCAAAAATATATGAGTTAGTTTCTTGGTCTATACGATCTATAACAAATATTTTGTTTTGATTATTATTAATAAATTCTACATATTGCTCATGAATCCCTCCGTATTTTTTAATTTCATTAATACTGTTTAGCTTAACCTTATCTCTTGGTCTAAACCTCATTATAATATTCTGTAAAAAATAAATCTGATTTTTTATTCTATTTTTTATAAGATACTTCCTATTAACGATAGGCTACACTATCTCTGATTAAATAAATCTACAAAAGTAGAAATCTTATATAAGTTCTAAACAATACAAGAGAAACCTCGAAGACGCTCATCTACAAAAGTAGAAATCTTATATAAGTTCTAAACAGGCCCACACAGGTGTAGAGCTTAACATCTACAAAAGTAGAAATCTTATATAAGTTCTAAACATTTTGAAAATATGATTGAGAAAAAAAATCTACAAAAGTAGAAATCTTATATAAGTTCTAAACTTATTATACTTTTTCACAGAATAATCAATCTACAAAAGTAGAAATCTTATATAAGTTCTAAACTTTTCTTCATATCTGCAAACTGCTAAATCTACAAAAGTAGAAATCTTATATAAGTTCTAAACTACGGTATTGCGTAAGGCTTGCCTCTGAATCTACAAAAGTAGAAATCTTATATAAGTTCTAAACTATTCCCTTCCTTATTCCTTGTCTTCATCTACAAAAGTAGAAATCTTATATAAGTTCTAAACCAGGAATAAGAGAAATAACAGTAATGATCTACAAAAGTAGAAATCTTATATAAGTTCTAAACTATGCAATGCGTAAAGGTTTTGTCCTTATCTACAAAAGTAGAAATCTTATATAAGTTCTAAACTTTTCTTCATATCTGCAAACTGCTAAATCTACAAAAGTAGAAATCTTATATAAGTTCTAAACACGAGATAGAGAAATTTGCTAAGGCTTGATCTACAAAAGTAGAAATCTTATATAAGTTCTAAACGGTTAATGGTTTCATTCTGATATTAAATCATCTACAAAAGTAGAAATCTTATATAAGTTCTAAACAAAAAAGGCATATTCCTCGTGCTTCAGAGATCTACAAAAGTAGAAATCTTATATAAGTTCTAAACAATCTTTGAAAGATAAGGCTTGGGTATCTACAAAAGTAGAAATCTTATATAAGTTCTAAACCAATTGTATCACGATCAAATGTTAGAATCTACAAAAGTAGAAATCTTATATAAGTTCTAAACCCGCTTTAACCGCCCTCATAGCATCAAATCTACAAAAGTAGAAATCTTATATAAGTTCTAAACTCAACAGTAGTTTTCTTTGGGTCGTTTTATCTACAAAAGTAGAAATCTTATATAAGTTCTAAACTAGAAACTGGAATAATTTCCACTATCTATCTACAAAAGTAGAAATCTTATATAAGTTCTAAACCTATAAAACACATCTTCCAAATTATCTGGATCTACAAAAGTAGAAATCTTATATAAGTTCTAAACTTTGCTAACTATATTCTAACTTAAATCATATCTACAAAAGTAGAAATCTTATATAAGTTCTAAACCAGAGGAATCTGACTACTTACGACAGATATCTACAAAAGTAGAAATCTTATATAAGTTCTAAACTCATAAATATCATGATCTGACAATCATTATCTACAAAAGTAGAAATCTTATATAAGTTCTAAACAATCAAAATATAAAATAAGCGGTATACATCTACAAAAGTAGAAATCTTATATAAGTTCTAAACGAAAAACTGAATCTAATGCTACTTTTGATCTACAAAAGTAGAAATCTTATATAAGTTCTAAACTATACCCTTAAGGGGAAATGACCTATGCATCTACAAAAGTAGAAATCTTATATAAGTTCTAAACTTGAAACCAGCGTTTAAGTCTTCAAAAACGATCACTGCATTATATTCCAAAACCAGCTCTACAAGCTTATTTACGACCTGTGAAATGTAGCCATTTTTGAGATCAGCGATTTTCTCAATTGTATCTCGGTTTTGACGAGCATGGAGTCTGTTTTCAGCTTTTTGAGAGAGTTTTGCTTCATAGTCGGTGCCGTTGAGGAGATTGAGCGTCCCTTGTTTGACAATTTTCCCAGTTTTCACCGAAATAATGGAGAAATATCAAAGATTTCTTTCTCCTCTATCGACTCAGGCCACATGGAGATCATCAAAATGAGGGATAATCAGCTCTTGATTGAGCTTCTGATTAAACTTTGAAGGAGCAGAAGGGCTGACCTCATTACTTCCAAATCACAAGGTAATCGGCACATGAAAAAGGATTTTATCTTCCGTATATCTTTTGTACTTGAAAACATCGTAGTTATCTACCTTCAGCGTTTTCTTCTCCTTTTTCATACTTCATGGTCTGAAGAAAATTTCTGCTTCTCCATTGAGTTTTACATTGGTTGGACTTTCAAAAAGCTGTTGCCAATAAAGCGTTTGCAAGTCAGGAGTCTTTGACTTTCCTTCAAAATCTTTGGAAGAAATCTGAAAGAGAAAGAGAGATCAGTCTTTTATTCCTTGTTTAATCAGTTCTTCATTAACCTTTCTCCAGTTTAACAAATAACCTTGTTGTTCAACTTCTCTATAAAACTGAGAAATATCTTGATAATTTTCTGTCGCTTGAAACTGGAAATTGAACACTTTCCAATCCTCATATTTTGGCAGTGCAGACTTATAAAAATCAATTAAAGTATGGAGGTCTGCGAGATTGAAGCTCTTTTCTGACTTTTTGAAACGTCCTTTAGCATAAAGGTCTAAAACCTGATCAGAAGCTCCGTATTTTTTCTTGTCAGAACCAGGCATTAGACATTTTGGAAGCATTTTATTGGCTCATGGAAGGAGCTTATAGTCCATCTTTTCCATCGTCCCGAGATTCCCCTCGTAGAGAGCCGATTTTTCAAAAAATTTATTCTGATCCTTTTTCATAATTGCGAGATAAAATTTCCCATCTTTTCTCAAAATCACGGATAAGTTCTGTGTTTCTTTATTTTTATCTCGTCCGTTCAGCAAGGTAGAACAGTCAAAATTCAGCTTTAATTTTGCTTGTGAATAGGGTTTTCTCGTGAGATAATTCCTGATCATATCATACCACTTTGGCAAAGGATAATCCTGCAAAAGCTGATCAAGACTCGGATAAAACTCTCCTTCTGGGACAAATCCAAGTTTACTCGTATCTACTTTGAACCACTTGAGCATCCTATATGCATACAAAGCATTGTCCAAAAGCATTTTAATCAGATTTTTGTGTTCTTGATTGTTGGATTCTAGAGTTAAAAGCGATTGGAGAGCGTTTTTTGCTGAAGTATAGGATCAAAATCCTCCCTCTCAAGTCCAAAAAGATTCAAAGTCAGATTTCATACTTTTGAGCAGAAGTTCCCAGTTAGAATGTGAATTTTCTAAATGAAGATTTTCTCGTTGGAGTTTGAGAAGAGAGACTTGATGATGATTTCCTTCGGCGGTATCGGCTTTTTCTGCTGCTTGAAAAGGAACTGCTTCCAAAAGTTCCTTGAGTTCAGCGAGCGAAAGATAGGCAGGAAGCTTGAAACTTTCTTCATCATTTTTTTTGAACTTAAAAAGCTTGAGCTCAACTCCCTTTTCTAGTAAAGTATGCCAAGATGAGAAGTAGCGGTTAGAAATGATATTGAGAGCTGTTTTGGACAAGAAAATCTGTGAAAGATCCGCCTTCTCCTCAAAAATTTGAGCCAAAAGCTTCTTAAATTCTGGGATTCTTTGATCTGTTTCTCTAATCATGGTGTGAAGCATCTTTTGGAACTCCGCTTCTGAAGTAATCAAATCAAAAGGAATCGTATTCCCTCTTGGAGATCAGATTTGTTTATAAAGGAGTTTTGGCTGAGGAAGTTTGGTTTTATGTTCTTGGGTATAAAGATTTGCTTTTGACCTAATATTTGCAAGCATTTCATTGTAAAAATCAATTCCTGATTGAGTAAGGTATTCGCTGTAAGAGTCAGGATTGAAGACTTTTTTCTCTAGTTCTGAAAGTGAAAGCACGGCAAGTTTCTCAAAAACCTCACAATTATTGGCAAATGTAAGGAGATTTTCGTTGATCGCACGATAAGCAACTGCGGTGGCTTTCTCATCGGTAGCATAGTAATTCTCGCGGTTCTGGTTGAAACCTGTGAAATAGGTAAAGAAATTTCCAAAACGGCGGATCAGTGCAAAGCTTTCTGGATCAGAGGTGTATTTCTTTTCCAGAAGTCAAAGCACTCCAGCTTCAGTCAGGATTTTATAACTTTTTTGAGTCAGAAAAGGTTTTCCTTTTTCATTGACATAGTCGGGATTGCTTTTTCGGAGCTCTGCAGTATTTGAAAAGCTCGTTCCAATCGCAGTTCTCAAGGCTTTTGTGCGGCTTTCAAATTCTTCATCAAGGCTTTTTAGCTCCTTTTCGGAGAGCTCAGCTTTGTTCTTTTTCTTTTTTTGGTAAGTTTGATAAAAAAGGACAAAATCTGATCGATTCTGGGGTTCTAGCGACTGAAGGCTTTCTGTGATAAATTGATTGTGAAGCTCGTCCAAGAGCGGTTTGATTGCCTGATAGTCTTTTTCTCTCTGTTCATCCCTCTGAGGAATTTCTGAGTTTCGGAGCGCACTAAGAGTTGGATCGTGTTCAAAATTTTCGTTGAGCGGTTTTAGCTCTCGGCGAAGGGTTTTTTGGATGGGATAGAGATTGGTGAATTGGGAGAAGAGGAGCTTTGTCATTATTTGTAAAAAAACAGATAAAAAAAGTTTATTTCAATTTTTAGTTTTTTAAAGTTATAGGAAAGAGAGGGAAATACGCTCCCTCTCTATAAAAAACTTAACTTTAGAAACACTGACTCAATTCTTCAATTTTTACTCTCCTCTGCTGCATAGAATCCCTTTCTCTGACCGTTACTGTTCCGAAGTTTGGAGAAGTTTCATCAACAGTCTCTGGATCAATAGTAACGCAGTAAGGCGTTCAGATTTCATCTTGTCTTCTATATCTTTTTCCGATCGCTCCTCCATCATCATATTCACATTTGAAAGCCTTACTGAGATCGCGGAAGATTTTTTCTGCGGTTTCAACCTGCTTTTGATCTTTCTTTACGAGTGGAAGGATCGCAAACTTCACAGGAGCGAGCTGCTTAGGGAAACGAATCACCACGCGGCTAGAACCATCTTCCAGAGTTTCCTCATCATAGAACTCAAGCATCGTAACCATCACTTGACGACCAAGCCCAAAGGAAGCCTCAACACATCGTGGGATGTATCTCTCCCCAGTATAGGGATCAGAATATTGCATATCTTTTTTACTGAACTCTTGATGCTGAGAAAGATCAAAATTCGTTCTGTTGTGGATTCCCTGAATCTCACCAAACCCTCGTGGATAGAGGTATTCTATATCTACTGCTGCTGCCGCATAGTGAGCCAGCTTTTCATGATCTTTGAATCTCAAATGCTCTGGACTAATTCCGATTACTTCAGTCCAATAGTGCATACAGTCAGCTTTCCATTGATTGTAAATCTCCATCGCCTTCTCTGCATCAGCAGGCACGAAATATTCAATCTCCATCTGCTCAAATTCTCTCGTCCTATAGAGGAACTGCCCTGGAGTAATCTCATTTCTAAAGGCTTTTCAAACCTGAGCTAATCCGAACGGAATCCTCATTCTCGTGGTATCCAAAACTGCCTTGAAATCTGTAAACAAAGACTGACAGGTTTCAGGTCTCATATAGACTTTGGAAGTCTCGTTCGCTACCGGTCCAAGATGTGAACTCAGCATGAGATTGAAGTTTCTACATTCCGTCCAATCGGCTGGGTTTCAGGTTTCAGGGTTATTTGGGAGATGCTTTACGATGAAGTCTCTCATTCCTTCGTTTCCTCGCGATTCTGGAGAAAGGTTTGGCACCTCTAGTTTTTGAGCCAATTCCTCATCGGAAAGTCAGTTTCTTTTCTTTTCAAAATAATCTTCAATCAACTTATCCGCTCTAAATCTCTGCCCCGTTTTCTTGTCATCAATCAACGCATCAGCGAAAGAAGCCAAATGTCCACTTGCCTGCCAAGTCGTGGGATGTGCAATGATCGCCGTATCCATAAAGACCATATCTTCTCTCTCCTGCGTGAAATGTTTGACCCAGAGATCAGCAATATTTTTTTTGAGCAAGGCTCCATAAGGACCGAAATCCCAAGCATTTGCCAGCCCTCCATAAATTTCTGAATTGGGATAGACGAAACCTTTTCTTTTCGCCCAAGCTACAACCGTTTCTAGTGATGTTTTCATCTTTACAATAGGAAATAAATTGAATACAGATTTCATACTAGTTATTTTTTATTTACTGTCAAGGGGAAGTCTAGCGCTTTTAGCATAACTAGCAATGAAAGGTTTTTGAGGAAAAGAAAAAGCTCCCCTTCGTCGCTAAACAATCCAGATTCTCTTTGACCGAAAATCAACTATTGTTTAGATGGAGAGCTTATTTCTCTAATTTCTTGTTTGTAAAGTTTTGAGAGGCTAAACTTCTACGACTTCTGCTGAATCTTTTGTTTGAGGATCAGGCTTTTTGTCTTTTTTCCTTGACCTAATGAAGGCTATAGCTCCTCCAATCGCCACAACCAGAAGTCAGATTCAAATAACAGGTCTTACGACAAGCCATGCTATCGCGATGGTTCCTAATCCTAGCACTAGTGTTGCCGCAAAAGCGATAACTCAAGTTCCAAAACCAATAAGTCCTGCGAGAGGAGGAAGCACTTTAGCGAGAGTTGGGATAAACTCGAGCATCATCGCAAATCCCATATACATCAACACAAGTCACACAAACCTTAGTATCCATGTAAGTCCCAGATTTTCAGCTTGTGCATCGGCAAACATTTCAGTAGAGCTTTTCTTGCCATTTGCTAGGAGAGCAAGGTCCACTCCGTTTGAGGTTTTATAGAGAGTTATTTCGTTTCCAGATTGTTTTCCTACGAGACTTACGGTTCCTGTTTTTACCACGGAGAATCTTATTCTCATGTCCCCAACTGCCGGACTATGAGAATCCAAGCCAACATAAATCATTGAGTCATCAACATGGAATCTTTTTTTGGACGAAGCTCATGATCGAGTGTTAGCGGATTCTGGAGTCAAAGCTCAGTTCGCTTGCTCTCCTGTCGAAGCATCTCCTGAGAGTTGCTCTGTCATTTCAGAGGTTTCAGCAGAGGTCTCTGTATTGTTATTCATCGAGCTTGTATATTCTGTTTCCTGCGCTGGAAGCTTCAATTCCTGGCCATTTAGAGGAAGCTTGACATCATCTGTAAGCTGAGAGGTAAATGCTTTATCTAAAGTAAAGGCTCCTACAATGATTGGTTCCTTTTCTCGAGTCTCGCTTTCATATTCTCGAGTCGCTGGATTTTGGTGTCCCTGAGTCTCATGGAATCTACTTGAATCAATGGCTGAATCTGACCACTTTTTCTTGTAGTCGTAGGTTGTGGTACAGTCCTGACTTCCACCATAGTTGTCATGACATTCTTCGCTTGAAGATTCTGATCGCTGATACATCTCTACGGATCTGATAAGCTTTAGATCATCAGCCTTAACTCCGAATCTTGGATCTTGGAGTGATGATGCAGGAGAGCTGGTCACCCCTTGGATGTGAACGAGTTTTCCATCAAGCTCAGGATTAAGCTGCTGTGCATCAGCATCTTGCACAAGTTTTTCTCCTTCGTTTAGTCCTACCTTCGTCACAAAAAAGTGGTTTTCATTTCGGGCTAAAAGAACGATTGAACCGATCACAAATAGTAGTCCTCAAAAAATCTTTTTGAAGCTGTTTCCTACACGTTCTCCATAGCCGGTTCTGGTAGTTACACTAACACGATCTGCCATTTTTGTATAAAAAGAAAATAAAAATACCCTACCATTAATAGGGTATTTTACAAGAAAAGCAAGAGATTTTTCTGATCTTTAAGGAGTTCATCGCTATTTTTCAGTGATTCTGTAGTTTAATCACAGATGAAATCGATCATCTCTCAGCAGGCAATCGAAGATTCAAAGGAGGAACAAAAAGAGAAATCTGATTTTAAACTCTTTTATACTGTTCCTCAAAGGGAATACGAAAACGTTTTCCCTGCACTCCATCTTCGGCACGGACTCAACATGCAACTACTAAACTCACCTCAGCTCCTTGAGGGAGGTTGAGAATCTTTTTGATCCTCTGGCTATCAAATCCTTCTAGCGGGCAAGTATCATAATTTTGCTCAGACATCGCAAGCATAAAGGTTTGAGCGACCAGTGCACAGCTCTTATGAACTACCGTTTGCATATCAGATTCGTATACCTCACGAAGTGTTGGCTTGGAGAAACCTTTTATTCGAGATACTCCTTTTCTAAAACATCCCAATACCCCGAAACAACGACTATAGAGGAAAGGGATTAACTTCTGATAATAGTCCTGGAGCTTTTTGGTCCTGGCAGGAATCCTGTCTTTGGGAGCATTGTTTTGGATTTCCTCGAGATTGAAATCAAAGACCGCTTTTGCTCTCTGCTTATAGAGGTCTTGACGAGTGATGAAAACGACTATTTCTTGTGCTGTCGTCGCCGATCTCTGGGAAAAGCATGCCTCTGCTATTTGAGTGATCACTTTGGGAGTCGTGATATGATAGGCTTCTCGGAGCTGGAGATTAGAACTCGTCGGTGCAAGCTGGGCAAGCTGGAGGCATTCTTTTACTTTTTCTGAGTCGATACGCTCGGTTTTACTGTAGCTACGAACTGCACGGCGATGCTGAAGCATTTTTTCTAAGGTCATTTTTTCATCAAATAGGAATATAAAAAGCTTTTAAAGACAGATTATGAAGAGAGGTAAACATATTTTCGGTAGGAAAGACTCCCTGCAAGCGTACTCCTCAAGTTCTAAGGATTGGAAGGAGTTTTTGCTAGTCGATTCCCAACGCAGTAAGCACTTCATCCTGTGGCGTTTTGGGGTCATCACGGAGAATAAGGTCTGGTTTTAGACCTTTTCCATTGAGGGAATCTTCCTGATCAGCGACATATCGTAGTGCTACTGTATATTTGAGGACGGAATTATCTTGGAACTGCACGACCTCTTGGACGGATCCCTTGCCATAGCTGGTCGTGCCTACGAGGCTCACCTTGCCTGGATAGTAATGTTTGAGCACGCCAGCGAAGATCTCTGCTGCACTCGCTGTTTTCTCATCCATGATCATCACCATCGGAACCGTAGGAGCTGTGGAAGCAACTTTTGAGGGATAGACTTCCTTGCCCTCGGAGGAGAGAATGGAGATGATTGGCTGATCCTTGGCGATAAAATTCTCAAGAATTGCCACGACTTCATCTACAATCCCTCAGGGATTCCCTCTCAGGTCAAAGTAAAGCACACTGCAGGATCAGAATTCCTCCTTTTCTCTCAGAAATTTCTTTTGGATTCACTTGTCAAAGCTGGAGATTTGAAGGTAGCAGGCGTTTCCTTTTTTGCGGGACTTGAGTAAAGGGAGCTCGATCTTCTCTCTCGTGAGATCGTAAGTAAGCTGGCTCTGGTCTCTTTTTACCATGATGCTGACAGAGGTACCAACTTTTCCCTGCAATGCGAGTATGAAGGCATCGAAATCATGATAGGATTCATAAGGTTTCCCGTCTGCTGCGAGGAGGATATCTCCAACTTTGATTCCAGCCTTTTGGGCGGGGCTGTTCTCTATGAGCTTCACGACAACTGGCAGTTCTCTTCCCTTTTGCTCTAGGTAAAGCCCGATCCCCACGAATCCTCCCTGCAAAGCGTCAGTAAAGAGTTTCCCATTTCTCGGGGTGTAATACTTGGTATACGGCTCTCCAAGCTTGTCTACATACGTAGATAGCTCATCATAGCTCGGAGCATCATGCTCGGCAATCTTCTCCTTTTGAGAGAAATTCTTTCTGAGGAGGGAGAGGACCTCTTTGGCGATGGTTTGCTTTTGTTGATCGGAGATCGTAGGTGTAGGTTCAGCTTTTTCCTCTTTGGGAAGAGCAGGTGTTTGGAGAGCCTCCTGCAGCTGCTGCTTGAGCACATCAAAAGTCAAAGGCTTCCCTGCTTGGAAAACGATCTGCTTTTTGAAATTATACTTGAGGAGCAGTGCTAAATCTGACTCCAGAGCCGGTTTCTCTAGAGGGAGTGCGGTCGTAAGGTTTGGGAAGGCACCGTTGGCTACGGCATTTTGTAGGAGCGGGTAGAGTGGAGAAGCTGGCGTGAGGTTGCTGTATTTGAGAAGCGGTTTCTGTGAGGAGCTTGGAACTTGCTGTCCAACGACAGTAAAGTAAAGACGGAGCAGTTCTCCATAGCTCATCCCCTGCTCCTGGGCAAGACTGACTCCAGCTGAGCAACAGCAGACAAAAAGTAGGAAGAACAGTATTTTTTTCATCAGTTGTGGAGAGAAAAGAATAAAGCACGATCGGTGCGTCAGTATAGGTATCATAGCAAGAAATGCAAGACTTTCTCCATCGTGACTGTGCCTCAGGCAAAGGAAGGAAAGCTCTTGATTTGTGGGGCAGATTCTCTATGATGAGTCCTCATATCCCAAGGTGATCGCTGAAGGAAGTCAGTCTTTTGAAGCTTTTTTTGTAAAGGACGGAAAGAGACTACATCGCCTGCATACTGGCAAAAAGCAAGCAACTTGGGGAAGCTTTTAGATGAAAAAGAGAGATTTGCTACCATGAAGAAAGGGAAAATCTATGAGGAACTGCTGGTGCTAGCAGACGCGAAGAACGCAGGTTTTGTGCAACGCTTGATCCCGAACCTCGCACCAGAACGCATCCTGGGAACTAAGACGCCAATTCTCAGAACGTTCGCTAAGCAGCTCGCCAAAAGACCGGAACGTGAAGCCTTCCTCAAAGAGCTCCCCCATTTCTCCTTTGAAGAGCATCTGGTGCATGGGTTTGTGATTGAGGAGCTCAAGGATTTTGAGCAGACGATGGAAGCCCTCGAACACTACCTTCCCTTTATCGATAACCGAGCAACCTGTGATCAGCTCCGTCCCAAAATCCTTGCCAAGCATGCGGACGAAGTGCTGGTAAAGATTAGAGGGTGGCTAGGAAGTGAGCATCCCTATACGGTTAGGTATGCTATTGGACTCTTGATGACTAACTATTTGGATGAGGAGTTTGAGCCTGGACTGCTCCAGCTCGTGGCAGAGGTAGACCGTGAACACTACTATATTCAGATGATGCAGGCGTGGTATTTCGCTACTGCACTTGCAAAACAGCCCGCATCTACGCTTCCCCTCATCGAGAGTCAGACTTTAATACCTTTTGTCCAGAATAAAACGATCCAGAAAGCTAGAGAAAGTAGGAGAATCTCCCCTGAACTCAAAAAAGCCCTCCTCGCATGGAAAATAACCGAGAAGGGTAAGGGCTAGGAATAAGGGAAAAACGGCTGGGCATAAGGCTTAGCCGATCAATCCCCTATTGATCAATTCTCTCTTGATATATTCTGGATTGTTGGCGGCTTCGATGGCTTGGTCAATGCTGATCATGCCTTTTTCGAGCAGGATCATCAAGCTCTCCTCCATCAGTTGCATCCCTGAAAGTCTATTGGTATACATCACACTTCTGAGCTGGTTCAGCTTATTTTCTCTAATAATATTGGCAACGGCGGTGGTATTGAGCAAGACTTCCTGTCCAAGCACGAGCCCTGTTTTGTCTGTCCTCTTGAGGAGCTTCTGCACGACAATCGCTGACATATTTTCTGCAAGCTGGGTCCTAATATGATTTTGTTCGTCGGAGGTAAAGGATCCGATGATCTTATTGAGTGCCTGCTCTGCCGACCTTGAGTGCACTGTCGTAAGCACCAGATGCCCGGTCTCTGAAAGGAGGATTGCATTTCTGATCCCTTCCAAATCTCTAATTTCTCCGAAGAGGATCACATCAGGTCTCTGCCTGAGGGCAAACTTCATCGCGCTAGAAAAAGAGGAAATATCCTTTCAGAGTTCTTTCTGATCAATAATACTCTCTTTGGGTGTGAAAACATATTCAATCGGGTCTTCAATCGTGAGGATATGCTTCTTTTTATTCCTATTGATCTCCTCTATCATCGCTGCCAAGGTGGTAGATTTTCCTGATCCGGTAGGTCCCGCAAGGAACACAATCCCATTTTCTCTATAGGCAAGCTCTCTGAAGATGGGCGGAAGGTTTAAACTTTTAAGGTCAGGAATCTCCTTCCTCAGCAATCTAGAAACCACCATAATGTGCCCCCTTTGACGAGAGATGTTCACCCTGTATCTTCTGTCTTTGTAGCCTAGTCCAAGGTCCAAACTTCCATGTTTCTCAAAATAATCCAGTCCGGTCTTGTTCATCAAGGTTAGCGCGATCTGGTTGAGCTGTTCGTCGCCGAGTTTCTCAAGGGTCGTATCTCTATACACCTCTTCCATAATCCTGAAAGTCGGTGGCTCATCATAGGTAAGATACATATCAGAAGCGTTCTTCTGGAGCATCAGGTCATTGAGAAACTCAATATACTTTACCGGATCAGTAAACTTCATAGGAGAGCAAGAGAAAAATAAAAAATCTGATTGCTTCAAGTATACAGCTTTCTGTCCTAAAGTCAAAAAATATAGCTTCTTTTTGTGCGGTGATGGGGTGCGTATTGAGGGAAAGATGGGAAAGGGTTGGGAATGGAAGATTGTCGCTGGAAACAAGCCTATGTTCGCTGTGTGCTAGGGTTTTGAGAGTATAGATGCTAGGGTAAAAAACGCTTGCATTATAGGTTTAAAAAACCTATAGTCGCCACTACGAAACCAAAAATCAACTCAATTCATCCTACTCATAACGATGATGATGCAGAATTTAGGGGTCCTTTGGATAGTCAAGCTGTAAGCATAGGAGTTGATACCCTCTCCAATCATCAAGGAAACTATGGAAATGTTGTGGATGTGAAGCAGGAAAGTTCAGAAGAAGGGAATTTTTACACCTCCTATTTGTATTTCGAAGACATCCCCCTTCAGTTCGTGCAGGGCAATCGGTTTTTGCTGGAGAAACAAAGCTTAGTAATGCTAGTATGAGCGGGGGAACATCAAGGAGTCCTCATGCTCACGATGCCCTTTACTTGAATAATGGAGCATGCTAGGAGGGAAAAGTATTTTCCTTTGATCCCTTCTTATCAAAGGAAGCGCTGGAGATCGTGCAGTCGTTTTGATCGTCGCTGGAGGTACTTCTTTCCAATCCACCAGCATAAAACCAGATTGCCCACCGAGAGAAGTATAGGACTCGCCCTTTTCTAGGTCTGCTTTCTTGTTTTTCCCCTTGGGGCATCGTCGTGGTTGCCAGCCCTCCTTGCGTTCAAGCTGGGGGAATGGAGGGATTTTGGCAGTGAGGATGGCGAGGGAAGAGCAAAAAGGGGGCTTTTTTTGGATTTTCGGGTGATTTTCCCTTGACAGAGGGTGAGGGATTGCTATAGTCGGGGTAAAGAACAAAAGGGAAAAGCTGGTTTCCAGCGGTAGGTAGCGTTGGTGAATGGGGAGAAAAACCTCCTGCAATAGCCTTGCAAGAGGTGCAAGGAGGAAGCGGACTCCTCTGCTCAGGGTTTGCACAGCGTGCAACGATAAAAAAGACCCCTCGCTGACAGTTTGCAGACCGTGAAACGGTGGAAAAGGGTCCTCGCTGACAGTTTGCAGACCGTGAAAAGGTGAAAAAGGAGCCTCGCTGACAGTTTGCACAGCGTGAAAAGATGGAAAAGGGTCCTCGCTGACAGTTTGCAGATCGTGAAAAGGTGGAAAAGGAGCCTCGCAAGGAGTTTGCAGACCGTGAAACAGTGAAAAAGGGTCCTCGCTGACAGTTTGCACAGCGTGAAAGGGTAAAAAAGGGGTCTTGCTAGCAACTTGCAGGGGGAGCAAACTCTGGGAAAGGGGTCTCGCTAGCAACTTGCATGCGCTGCAAAGGGTAAAAAAGGGGGCTGGCTAGCAACTTGCAGGGGGAGCAAACTCTGGGAAAGGGGTCTCGCTAGCAACTTGCATGCGCTGCAAAGGGTAAAAAAGGGGGCTGGCTAGCAACTTGCAGGGGGAGCAAACTCTGGGAAAGGGGTCTCGCTAGCAACTTGCATGCGCTGCAAAGGGTAAAAAAGGGGGCTGGCTAGCAACTTGCAGGGGGAGCAAGCTCTGGGAAAGGGCGGTTGAATGTCGTGGGTAGAAAAGTCGAACTTCAGAAATACTTCAGGTTTTTATTTTCTAATGATATTCCCATGATGGAAAAGGAAACTTTACAGGTCTTCGGAGTGAAGAGCCTGGCGAATGCGAGCTATGGGGCGTTTATGGAAGCGGTGAAAAGTCTCATCGCGAAAGGGACGACCGAGAAGCTCGGGCTGAAAGCGGAAGATTTTTCTGCTTTTGAAGCGTTGCTTGTGCAGCTTCTTGAGGTCAACAACAGCAGCAAGAAGGATGCCACGACCAAGGAACTTGATGCGTTGGATGAGCAACGTGATCGCCTGTTGACGCATCTGTTTGCGAGGGTGGCGCTCGAGGCGGGGTCGCCTGATGAAACTAGTCAAAAAGCCTGACTTCAGCTGATGGGGCTTAGAGAACTCTACAAAGGGATTCAAAGAAAGCCGACTCGCGAAGAGACCTATTTGATTAAAGGGCTGTTGGTAGACGCTGGCAAAGCGGAGCATAACGCGGCGTTTGTGAAGCTCGGGCTTAAGCCGATGCTCGACCAGCTCAAAGCGATCAATGAAGACTATGAGAAGAAGACTGCCTCCCGTGCAGCATCTCAAGTAGCAAACACCTTGACTCCGGCGAAGGAGCTTCGCAAGCAATTGGATCAGTATTATGAAGCGTTTATGATGAAGATTGGGGCGTTGGCGATTGTCAGCCCGAATCCAGAACTAGAAGCGTTTGTAAAGACGTTTAATAAGCTGGCGAAGGACACGAGAGAACGCTGGAAGTTGCACCTTGCTCAGCTCGGGCTTTGGACCGAAGAAGGTGGGCGAGAGGACGAGGATGGCAATGCAGAAAGTGAAACCGAAGCAGAGAAATAAGTGCTTCGCATCAAAACCTCTATGAAAAAAAGTCTGACCTGAGATACCAGAGTCAGACTTTTGGGATTAAAGGGATAAGCGAGGCGATTGCTCAGCAAGAAGCCTGAGATTGAGAGAGATCGTGATGACTAAAAAAGATCGCATTCTTGTCCCGTCTCCTTGCAGTATAAGAAGCTAGGTAAATAATTAGATCATCTCCATAAGGAGCTTCCATGCCGCCCTCCAGCCAATAGGTATAAAGCTTCTGTTCTTGCTCATCTAATACCAAGCACTCCACTCCAAGGGGACTTGGAGCACGACGATACGTCACACAATCCTCAGTAAGTGGACATGAAAGCACTGTTTGTAAGAGTTGCAACACCTGTGCTTGCTCCTCTTTACTTGAGTTTCTCCACCGATTATCGCCTCTTTTGTATCTTTCATCACAGGGCAATCCCTCAGGAAGGAACTCTCGAACCCCTGCTCCAGCAAGGAACTCTTTTGCTAACCCAAAATCTTCATTTCCCATAATAGTTTGTTGTTGTGTCCCTTCGTGAGAAAGGACGGATTATTATTGTTTTTATTATTGTGCCACTACTATAATCATTTATTACCCAAATGTCAAGCGTTTGTGAACTAAAAGAAAAAAGTCTGGCTCCGAGATACCGGGGTCAGACTTTTGGGATTAAACGGATAAGCGAGGAGCTTGCTCAGCAAGAAGCCTGTGGATGAGAGAGATCGCAATGACTCAAAAGGATCTCCTCCTTGTCTGGTCTCCTGTCAGTATAAGCACCCAGGTAAATAACTAGATCATCTCCATAAGGACCTCTCCTGCCGCCCTGCAGCAAATAGGTAAAAAGCTTCTGTTCTTGCTCATCTAATACGATCAACTTCATTCCAAGGGGACTTGAAGCAGGAAGATACTTCTTACAGTCCTCAGTAAGTGGACGAGAAAGCACTATTTGCAAGCGTTGCAACACCTGTGCTTGCTCCTCTTTACTTGAGTTTCTCCACCGATTATCGCCTCTTTTGTATTTTTCATCACAGTGGGACCCCTCAGGAAGGAACTCTCGAACCCCTGCTCCAGCAAGGAACTTTTTTGCTAACCCAAAATCTTCATTTCCCATAATAGTTTGTTGTTGTGTCCCTCTATGAAGGGGGACGGGTTTGTATTGTTTTTATTATTGTGCCACTACTATAATCATTTATTACCCAAATGTCAAGGGTTTGTGAACTCAATGAAAAAAGTCTGGCTCCGAGATACCGGGGTCAGACTTTTGGGATTGAGAGAGGAGCATCTAGCTTCTCAATGGGTGGAGCTGTCTCTGAAGGATGCCGCCCACCGTATCAAGAAACTTACCTGATCCTCGCCTGATATCACTCTCAATATCATGGCAGATCCGTGCTATCGCACGGTTGCAAAGCCGAACACAGTTTTCAAAGATAAGGGTTAGAATTTTATTTTCCTTGTGATTTAAAGGTATCGGCTCCTTGAAAAAATCTCCAACACAGATGACATGTTCTGAATAGTCCTCATAAAATGGATGATTCAACATTTCTTCCCGTGCGTCAATGAGTGCTATTTGCTCCTCTTTACTCAGCACTAGCCATTGGCGACTTGCTTTGGGGTAAAGTCTGAGGGTAGGATAGGGAATATTACTAAGTTCGTCAATATCAAGCTTCTCTGAGCACGGTTTGGTCTGTTTAATTTTTTTTCCCATAATGATGTATTACTATGTCCCCTCTGGGTGGAAAGGACAGGTTACTCTTATTTTTATTATCACGGGACTCACTATATGTATTCGTTCCGTAAATGTCAAGGGTTTGTAAACTCAATGAAAAAAGTCTGACCTGAGATACCAGAGTCAGACTTGGGGGATTTTTGATACAAGCGAGAGAGAGTCTCAGCAAGAACAGGTCTTCCGAAGAGTTGCGGCTAGGTCGTCAAGATGAGCAAGGTATGCCATCCTGATCTCGACGTGGGCATCAAGATAGATTTCCAACTCCTCTCTATAAGGATTCTCAGTATGGTGATCACGAATAGCTGTCCAAACTTTCCTTTCTTGTTCGTTTAGCACGACCGTTTCCTCGTTGAGTCTGCGGAAAAGAGGAGTATACTTTATATATTCCTCAGTAGCAGGGAGCGTGAGGATTGATTGCAGACAAAATAAAAGCTCTGCTCGCTCCTTTTTGCTAGACATCTTCCATAGGCTATCTGCATACCCAAACTCAATACCATGAGAACAGTCTGCGTCCATAAACATAAGAACGCCAGCTCCACTCAAAAACTCCATTGCTAGCTTACAATTTTTCTCATTCATAATAATGTGGTGTTGTGTCCCTCTATGATGAAGGACGGGTTTGTATTGTTTTTATTATTATGCAACCACTATAATCATCTCCTACCTCAATGTCAAGGTTTTGTAAACTCAATGAAAAAAGCCTGGCTCTGAGATTCCAGAGTCAGACTTTTGAAGCTGTGAAGTAAAGGAGACGAGCTCCTTACTTTGCGATTAGCATCCTGAAGGATTGTTTCAACCTTGCAGGGCTTACTATATTGCCCGATCTAATGGTGTAGTGAGCAACAAGCAGATCAAGCAGCACATTGACAAAAAAATTGTCAAGCTCGACTCCCCTCAGATAGGTAATAAGCTGCCTTTGCGAGTCATCCAAGACGAGTGTCGGTTGGTCGGGATGGTCGGAGAAGACATAGCACCCGATGCATTCCTCGGGAAGCGGTTCCGAGAGTGCAAGTTGTAGGATCTGAATAACCCCTTCTTGCTGGGCGGGAGATAGCTTGTTCCAGCAGGCATCACCGCCGTGGTAGCGATCATGCGTAAAAGAACGAGGGTGAGTAAACTTACGGACTCCTGCTCCAGTGAGAAACTGCTCTGCTAAACTTAAATCACGAACTCCTCCAATAAGGAATCCCTCTACTGAACTTAAATCTTTATTTCCCATATTCATTTGTTGTTGTGTCCCTCTGCAAAAGGGGACGGGTTTGTATTGTTTTTATTATCGTCGGACTACTATAATCATTTCCTCCCCAAATGTCAAGCGTTTGTAAACTAAAAGAAAAAAGTCTGGCTCCGAGATTCCAGAGTCAGACTTGGGGGATTTTTTAGTGCAAGTGAGAGAGACGCTCAGCAGAGGACCGGTTCTCGACGGACATTATTCTGTTTGGCAAAAGAGTCGGGGTAGCGATTCCTGATCTCGACGTGAGCATCAAGATAGGTTTGCAAGATGACTCTCTCATAAGCTCTGAGAGGTTGATCGTAGATAAAGGTCAGAAGTTTACTTTCTTCCTCATTTAAAACTACCCGTATCCCTTCAAGGGCTCCAGAGCTAAGGAGATACTCAATAGGCTTCCCAGAATATGGCAAGGCTAATACTGATTCCAACATTTCTAAAAGGGCCTCTTGCTCCTTTTCACTCAATCTCAGCCACGCATGGTTTGCCAGCAGGTAAGGATTGGACAACGCGCTCCCTTGACCACCAATCATAAGAACTCCTGCTTCCCGCAGGAAGCGTTTCGCAGATCTGCAGTTTTTATTTCCCATAATAGTTTGTTATTGTGTCCCTTCGTGAGAAAGAACGGATTACTACTGTTTTTATTATTGTGCCACTACTATAATCATTCATTATTCGAATGTCAAGGGTTTGTAAACTAAAAGAAAAAAGTCTGACCTGAGATTCCAGAGTCAGACTTTTGAAGCTGTAAAGTAAAGCAGATGAGCACTCCTCTATGCAGCTTATCGAGAAGCCATGCGTGTCAAAGGCATCTGTTTACGGAACAGGCGAAGATACTGTTTGAAGTAATGCTTCAGATTGACCGGAGTGAGCTGCCTGGGGCGATTCTTCCCCTCAAATTTACCGAGGTGATGGACAACAAGCAGATCAAGGATAGCCTCCACGATAGGAGTATGCATCTCAAGCTCTGCCAAGAAGGCTAGTATCTTTTTTTCCTCATCATCCAGTGCCATCTTCACCACTCTGAGATAACTGAGGAAGGGTTTTATGACCACAGACTCCTTGATTGATGGTCCATTGAGAGCATCCGCCAGCATTGCGATGATGTTAGTCCGCATCGTAGGGTCCAGACCCTGCCATAATTTATCACCGGGGCTCCCCTTGACTCTTTTGCAATCTCTACAACCAGGGCAGGAAACTCCTACTCCCGATAAGAAACGGTAAGCTTTATCAAACGTTTCTTCTAGTAATTCATTATTCTTCTTTCCCATACTCGTTTGTTGTTGTGTCCCTTCGTAAGAAAGGACGGATTACTACTGTTTTTATTATTGTGCCACTACTATAATCATTCATTATTCGAATGTCAAGGGTTTGTAAACTAAAAGAAAAAAGCCTGACTCCGAGATACCAGAGTCAGACTTGAGGGATTGAGAGATGAAGAGATTAGTGCTCCTCCGTCAAACCCGACGAAATGTAGATTGTTCAAAGGACTCCCGCATAGTCTTTAACCTGTTATCTGCACTGGCGATGAGCTCCACGCTGTTTTCGAGCGGTTGCTCGGCATGCTCCGTCCCCCCATATTTATGGTGGTGACGTGCGCAAAGGAGCAGAAGTATCGGATATTCATCAGAATAGTCAATATTTGTCATATTCAAGAAGAATAAAACCTTTTTTTCCGACTCATCCAGAGCTGTCTCGAGCTTTTCGGAATATGCAAAGGGGACATGGAATACCTCAGCTCCCATGGCAAGTGGCCTGTTGAGAGCAGTCCGCAATGTATTGTAGACCATGTTCTGCTTTTCGAAACCGAGCCCCATCCAGATATTCTCAGCTCGACTACCAAAATAGAAACGCAGTCCTATCAAGCTTCTCTCCTCTCGACTATCAGGCTCTCCCGAGCATCCTTTAAGTAATACCTTAGAGATCCCCGCTTCCTCCAGGAATTTATACACAACATCACACATTTCATTTACCAAGGTACATTTTTGAGTTTCCATAATAATTTGTTGTTGTGTCCCTCTATGAGAAGGGACGGATTTATATTCTTTTTATTATCACGGAACTACTATAATCATTTATTACCCAAATGTCAAGGGTTTGTGAACTAAAAGAAAAAAATCTGGCTCCGAGATCCCAGAGTCAGACTTTTGAAGCTGTGAGGTGAAGAGATGTGATCCTCAACGGGAGAGACCATCTCTAATAGCTTCCTTTAATAGGGAGGGGGCGATCATATATCCCAACCTGATCGAGTTATGAGCAACCAGACAATCTATTAAAGCCTCTACCACGGGAGATTCTCTAATAGTGGACTTCTCCAAAAAGGCAAGAACTTTACGTTCTGAGAGATCGAAGCCGGTCAATACCTTTTTGTTGCCTTTAAAGATAAGGCGTTCTCTTAGGTAGGTCTGGGGAAGAGCTGAAGAAAGAATAGCTTCCAAGAGATCTAGGAATGTTCTTTGATCTCTTTCATCCATTTCCGACCACGGCTTATTGCCAGCTTGAGTGCAATAAAGGGCAAGATCATCAGGTTTCTCTATTTCCCCGAAGCCAGCTCCCCTTAAGAATTCCATTACTGCCCCGAACTTTTCTTTTTGCATAATGATTTGTTGTTGTGTTCCTCTGTGAAGAAGAACTGATTTTTATTATTTTTATTATTGTGCAATGACTATATGTATTCGTTTGGTAAATGTCAAGCGTTTGTAAGTCAAAAGAAAAAAAGCCTGACCACCCTCCTTTGAGCAGTCAGACTTTTGGCTTTTAGTCTCTCTTAGCCCACACTCCCCTCCATCTCGAGCTCAATCAAGCGATTGAGTTCTCCTGCATATTCGAGAGGGAGCTGTTTCACCACGCTGGAAACGAAGCCATTCACGAGTAAAGACATCGCCTTTTCGGTTTCTAGCCCTCTACTCATCAGGTAGAAGAGCTGAAATTCATCTACCTTTCCAGCAGAAGCCTCATGAGAAACCAAAGCTGAAGCATTATCACATTCAATCACAGGAATCGTCTTACTCACCGACTCCTCATCAAGGATCAAAGCATCACAGTTCACGCTACATACGGCATTTTCAGCGTTTTTTCAGATTTTTACGAGTCCTCTATAGGTATTGATTCCGCCTTGGAGAGAAAGGGATTTGGAGATGATGGTTGAGCTGGTGTTTTTGCCGAGGTGGATCACCTTGGAACCGACGTCCTGATGCTGTCCTTTGGACGCCATAACGACCGACAGATTGTCTGCTTTGGCTCCCTCGCCCTGCAAGATTGAGCAAGGATAAAGCATCGTAGCTCCAGAGCCGAGATTTCCACCGATTCGCTCCATTAGACCAAAATCCTGAACCAGAGCTCTTTTGGTATTGAGGTTGTAGGTGTTAGTGGACCAGTTTTCAACTGATGAATATCTCATTTTCGCCTCCTTCCCAACGAAAATCTCTACGCATCACGCATGCAAAGAAAGCTGATTATACTTGGGAGCCGAACATCACTCAATATACTGGGCTTCTGCCTCATCTTCCACGATAATCAGCGTATGTTCAAACTGCCCTCCAGCGAGGGTATTCATACGGAAATAGGCTTGCAAAGGCTGGGTCAGCTTTACCCCTTTGGGAATATAGACAAAACTTCCCCCACTCCAAACCGCACCATGCAAGGCAGCGAATTTGTGATCAGTCGGTGGAACGAGCTTCATAAAGTATTTTTGGACCAGCTCAGGATAGGATTTGAGGGCATGACTCATATCTTCAAAGATAATCCCCTGCTTTGCTCGGACTTCCTTGATCTTGTGATAGACCGCTGTAGAATCAAACTGCCCTCCTGCTCCAGCAAGGTATTTTTGCTCTGCTTCAGGGATTCCGAGCTTCTGGAAAGTAGCTTTGATCTCATCTGGCACTTGTTCTCGGTCATTAGCGTAGGTTTGGGATCAGGTTTTTGGCTTTGCATAGTAAACTAAAGTATCAAAATTCAGATCAGAAATCTCTGGTCAGAAGCTTGGCATTGGGAGTTTATGAAAAATCTCAAGGGACCTGAGTCTCTGCTCTAGCATTCGCTGAGGCTCTTCAAGGTCAGCGGAGATTTTCCTCACGGCGATTTCGTTTAGTCATTGCAGGTAAAGGTCATATTCTACTTGGTTTGCGGCATCTAAGTTCATTATTTCAGCGGACGAATTAAAGGTCGTTTGGAGTGTAAGGAAAAGAGAAAGATTCTCAATCTGATTTTAGTTTTTGAGATTTTTTAGCTTCCAGGCTCAAATGATTGCTGCCGTCTCCATTCTCAACACGGACTCTCAAAGTGAAGCTCGTTCAAAGCCCTTTGGAAAGCTTTGGTAATCCCTCTCGGTTAGTCCTCCTTCGGGTCCCACTACCCCTAATAGAGGCAAAGCTGATGCTTTCGATGAAGTTGTATGCTGTTGCGTAGAAGGAAGGTCAAAAACGATGAATCTCCGCTCTTGATGCAGGCTTTGAGGAGCTTCAATAAAGCTTAGTTTAGGCAAAGATCGATTCCAAGATTGCTCTGTTGCCTCTTGCATAATTTTGGAGAGTCTTTGGAGTTTATTGGGATTTATGGCTTTGAGGACGGATCTCTCACTAACCCAGAGGTAGATTTCATCAACTCCTATTTCGGTAAACTTCTGGATGATCAGCTCGAGTTTTTCCTGTTTATTCGGCAAGGAAAGCAACATTCAGGTTTTTAGAGCTTTGGGAGGCGCTGGAAGGATTTGCACGATTTCAGCATGGAGCTCTTGATCAGATCGCTTTTGGAGCCTGAGAAAATATCTCTCTGTCGCCTCAGGAGCCTGAACAGCAAATTGATACCCCATTTGAGCCCTGAGCACTTTTCTCAACTGCTGAATAAGTTCAGGATTCCTTTTAATGATAAGCTGATTTCCAGAGACTTGAAACTCTGGACAGATAAAAAGCTGCATAGCGAGAAGAAGCAAATAAAAGCTGTCTTTAGACTACGATTCTTGCTTATTTTTTCAAGCGGAAAGCATACGTGATTGAATTAGAGAACGAAGCTTTTGTGTGCCTTTTGATTTTTTAAAGCAGCATCTAAGGAAATGCTATAGTATTTTTGCGATAGCATGATAATGAAAAAAGGGCAAATATTGCCCTTTTCAAATTTTCCAATAATAGGTATGCACTGAAAGATTGCATCCATCCTCAAAGAGAGAAATCATATATTTTCTACTCCGATAAGGGCAACATCGACTGATAACAATCTTATCTAGATATGCACCATTAGGGAGCTTCCAATGAATCAGGATTTTCTCCTTCCGATCTCTCATATTGAAATATGCAACTTCAACAAGACTAGCAAACTCTTGGTATGAGAGACCATAATACTCTCTTACCTCTTGCATGATTTTTTTTAAACGTTCTTTTTCCATTGTTGTTATTTTTTAGGATACTCGGTGAGTATAGTCAATCCTTCTACAAATGTCAATAGGAAAAATAGCTAGGTCTCGTATCTCACAGGATTCCCCTTATTGTTAGTCAGCATTTAGCTTTTATTGTTTGATGATTGCCTCGATAGCTGCAAATGCCCCTTCTTCCTGTGCATTTACTATGATATAGAGTTCATCACCTGGAGTTCCTGAATAGAAGCTCACAGCTGCTTGATTGAGGAAAAATATCTTTCCATTGTCAGTGTTAGCGGTATATTTATTGTCGTCAGTCCTCATAAGGGTTGCTTTTAAGTGTTTTCTTTCTACAGGTCTAATGAGCTTAAAGAGAAGCTTTCCGTCATTTAGAATCTTGAGCTTCATTACGTCTCCTGGCACCAATTTACTCTTACTAGAGTAATTTACTGGCACTGGATACTTCTTGTGATCAGCTCCGACCATATAATATCCGTCAAATACTCCTTCAATGACTTCTCCCTCTTCAGCTTTATAAACCTTTAGCTCTTCAGGATTCGTTGCTCTTACTTCCTCTAGGTAAGCTAGAAGAGATTCTGGATTTTCTGGATCAAAAGAAGCAAGCTGATCCAATGTAAGTTTTGCTCTCTTGAGCTGAACATCTGCAGATTCAATCGCAGACTGAGCGAACTGGAGTAATTGATGATAATTTTTCATCAACTCTTCGTTTGTTTTTGTTTTTGGCATCTTTTCTTTTTTAGATGATAAAAATCGCTTTATCATTATATTATTTCTTGTCAAGAAAGCAAACTTTTTCAGACTTTTTTATTTTTCCCGCTGAGAAGTGGGATTTCTTTGCTAAAAGCTCGCATCAAGGAGAAAGAATATTTTCTCTTGAAATCTTCTAAGTGAAATCTATGCTTGAGTCAGATTTATTTTCTTTTCCTTTGTATCATGTTCAAAATTTCTTATTCTCCAGATCTCAGAGGCGAGGTTCTTATTTCAGGGAGCAAGAATGCTGCACTTCCAATCATCGCGGCTAACTACCTGATTGATCAGCAAATTCAACTAGAAAATAAGCCTGATATTAGTGATGTTCGTTCGATTGAAAAGCTCGCAGAAGATGCACTCAGCAAGTCAACAAACTTCTTTGATCTTACCTCAGAACTCGCAACCAAATTTAGGGCATCTATCCTTTTAATTCCTGTTGGGCTCAAGAAATTTTGAGAAGTCAGATTTGTAGGCTCTGGTGGCTGCAAAATCGGAAAAAGACCGCTTGATACCTTTGAAGACGCATTGCATAAAGCTGGGGTTGAAATAACTCATGGGCAGTTTAAAACTTTTAAAGTAGTCGGCAAGCCAAAGAAAAACATTATGCTCCAGGAATTTTCTGTCACTGCAACAGAAGCACTAATCACGTATCTTGCATTCTTGGATGATGTAGACTATGAAATCACCGTTTATCAGGTAGCAACTGAACCTCACGTCAAAAATCTGATTGATTTTCTCAATGCAGCGGGAGCTGATATTCGTATGGGGATAGATCATACCATTACTGTAAATCCAAAGAAAATAGCAATCAAAGAGCCTTCTTTTAAAATTATTAGCGATTATATCGAGGCGGGGACTTATTTTGCGATAGGGGCATGAGCTGACAATAGCGAACTCATCATTAGAAATGTGAATGTTGACGATCTTTCAGCAATGTATAATATTGCGAGCAAGATTGGAATTAATTTTAAAATTCTTGACAAGCAAACACTTGTTGTCAATTCTTATAATAAAGCAAACTATAAAGCGGTAAAGCTTGAAATCAGAATTTTCCCTTGATTTCCTAGCGATTTGCAGTCTATTTTTGGTGCGTTAGCGAGCCAATTTGATGGAGTAACAAAGATTTTTGAAACTCTGTATGAAGGGCGTTTTGGGTACCTTAATGAACTGGAGAACCTTGGTTGAAAGATAGAAATTCTGAATCCCCATCAGGCTTTGGTTATATGAAAGACAGAACTTCATGGTGGATATGTTTCCAGCACAGATCTGAGATGATGAGGAGCAATGGTGCTTGCTGGTATTATGGCAAAGGGGGAAACGATGATTATGAACGAAGATATTATTGCTAGGGGGTATGCTGATATCCTTAATAAATTAAGCACTATTGGAGTAAAGATATCCGCGCTTCATTAGAAAAAAGGTAGGATCTATTATCCTACCTTTTGATGCTAGGGAAAATCCCTTCAACGGACACGCAGGAAATACTTTCCTGCTTTTTTTCTTGGTTCGATCGTTTTGATAGAAGGATTCAGCTTTCTTACCTGTTTTCTTAGATAAGCCTCTGCAATTTCCTTGATTGCATAGAGCTCCTCTATTCTTTGGATCGCTTTTCTGTAGTTCTCATTCACCTTGAAAGCTTCGCCCGCATAATTAGGGATGCGTCGAGACACTCTTTTGAGTAGAGAATCTCTGAGATCTATCGCATCGTTGATTGCGTTTTGGAGTCTGAACAGCAAACGATAAATTTTGAGGATATTCTCAGGCATTTCTACTCCTCTTTCGCTGCTTTTCAGCTCAGGGAAAGGCAAATCAAAACAGGAGTCCTCTTCCTCTTGGTAATAGAGGGCGAAAAATGCTCTCTTTGATGCATAAGAGGGTTTCAGAGAATAACAACAAAACCATTTGATGAAGCCGTCGATCAGAGTTTTTACTTTTAATTTTCGTTTTTTATTCATAATTTTACTATTTTTAAGTTTGGTTTTCTTTATATCTAAAATATATAAAATGTCAATATTTTCTCTTGATTTTTCCTTCAATTTTTATATAGTGATGTTGCTTTAATAAAAAAAAGAAGCATTAACGCTTGAAAGTTGAGCCTGGAAAGTCCAAGTAATAACGAGTAGGGCATAGAATTTAAAAAAATGAAAAAGGAAAAAATTTTAGTCGTTCACAGTTTACAGGATGCTCAGAGTCTGAATCCTGAACTGAATTCTTATGTGGTGATTCTAGGCTATACTCCGACGCTTACCGGCGAGTGGAAGAATTGTGAGGGCAGTTCTCTTCCGTCTTCTCTTGACGCGTATAAAGGAGAACCGGTAGTAATCGTCAAAATTACGCCTCAGAAAGTGAAATGCTATGCTTTCCCTCCCAGGAAATCGTATTGCTCGACTGGAACCTATCGTCAGGTACTTGAACGAATATAAGTAATTCTGATTCGTAGGCTTGAGAGATGCCCCTCTCGGGGTTTAAGGACCAATGGTAAGATATACCTTGGTCCTTTTTTTGTCTTGGTAAAAATGTCAAGCAAAAGCTGAATCAGACTTCAGTTTTTTTTTGAAAATGCAGAAGTTTTCTCTATACTTGAGTATTGGTTATATTTCATGATTTAATCCATAACTTTATAATATCAAAAATGCAAATTAGTATCTGATTTTCTGGACCGGCTGGGTCTGGAGTAAATACCGCAGGAATCCTTCTTGCTGAACTGCTTGCCCAAAAAGGCTACACCGTGCTCGGTGACAAGGAATACGCCTCTATCATTAAAGGAGATAACAACTGCTTTTTCCTGTATATTTCTAATGAGGAGCAGCCCTTTATTCGTAGGCAGATTGATTATTTTCTCGCCTATGATCCCTATGCAGTGAGCAAGAATGAAAGCATCTACGATTTGAAAAAAGTCTTTATGATCAAAGATGAGAAGTGTAAATACAAAAACACCTTTACTCTTGGAGCGGCTCTCAAAATTTTTGGAATCAGCTTGGAGGAAGGGAAATCGCTCTTGGAGCAGCAATTCTCTGAAAAGGAGTTCTCCCCTGAGGTGATGAATCAGAATTTTTTGGATTTGGAAGCAGGTTATCAGTATATTATTCAAAACTGTGGTGAAGCCTGTGATATTATAAACTGCAGTAAAGCAATCTGAACTCCGAAGCAGTTTATGTACGGAAACGAGCTCATTGCAAAGGGAGCAATCGCAAGCGGACTGGATTTTTACGCTGCCTATCCGATGACGCCAGCTTCCTCTATCATTGATGAGGTCGTAAAAAATCCGAAAGTAAGCTTCTTTCAGGGAGAAGATGAAATCGCAGTCAGTATGGCGATGCTCGGTGCGAAATTTGCTGGGAAAAGAGCGATGTGTGGAACCAGTGGAGGCGGATTTGCACTGATGACCGAGTCTATTTCCTTTTCAAATCAAGCGGAACTTGGGGGCGTTTATGTGCTTGCTCAGAGAGATGGTCCAAGTACTGGAACTCCGACTTTTACGGGACAGGGCGATGTGAATTATGCTTTGCATGCGAGTTTTGGAGACACGAAGCCGATCGTGCTTTATCCCTCTACTTTTGAGGAGGCGTATTCTTTCGCTGGGAAGGCTCTCAATTGGTCGGATCAGTATCAGCATCCTGTGATTCTCCTGAGTGATAAACAGCTTTCGGAGTCTTATTTGAGCATTGATCCGCACAAACTCCTTGTCGAACCGATAAAAAGAGGTAAAAAAGCTGACTCCACAGCCGAGGATCAGTTCAAAAGATACGAATTTAGTCCTGATGGGATTTCTCCTTATAGTGTGCCGTGAACTGAGCATACGACCTTCATTGCGACTTCCTATGAGCATGATGAATACGGAAGCACCAATGAAGAACCGACCATGAAAGGCAAAATGATGGAGAAAAGAGCTCAAAAACTGAAGACTTTCGTGGAACAAGAGTTTAATTCAGATTTTTATGGCTATGAAATCATCAATCCTGATGCGGAAATTTTCTATATCACGATGGGAATCAACCGTTATGCCCTAGAGCAACATATCAAAGACAAAGCGGGAATGGGGCTGATTGTCATTAAATCGCTCTCCCCTTTTGATCCGAGATTAAAGACTTTCCTTGAAGAACATGCAAAAAAGATTAAAAGGCTGATTTTTGTAGAAATGAATCAGAGTGGAATTTTGGAAGATTTGGTGCGTAAGGAATGTGAGCTGTATGGCGAGTGGAATAAAAAAATAGAGCATTTTAGAAAAATTACGCTCTATCCGTTCTTTGAGGAGGGAATTATTTAAGAAATCCGCTTTAACTTTGAGGAAAGGAAAGCAATGCTGTGAGAAGTTTCGCTTTTTGAAAAGGAAGACCTAGGGATGATTTAGTAACAAACCTTCTCTTTAACTTAGAACATAAGAATGAAAACCATTACTATTCAAAAAATCAATAAACAAGATCACGAAGCAATTATTCTTCTCTATCTTGAAAGCTACCTTGCTGAGTGAGAAAAATGGACTCAAGAACAGATTACTCCTTTCGTAAACTATTTACTCAAAAGACCCTTTAAGCTCAAGGCTGTGGTAGATGGGAAAATCGTTTGAGGATTTATTTCAGATATTAAGCCACGACATGAAGGGAATATCCTCTTTGATCCAGAAATCTTTATTCATCCTGACTATCAAAAACAAGGGTTTTGAAAACAGCTGTTGCAGCAAGCCTTACTTCAAGCTCAACAAGCGTATCAAGCTACTGATTTAATCGCATTTACTTTCAAAGATTCTCATCAACTAAAACGATATCAAAAGCTTGGCATTAAAACTGATGATTCTTGGCAAATGTTGTATGGAGCTTTAGCCCCTGTTCTTGACAAGTTATCAGAGACTTCTCAATGCAACATCAATGCTAATTAAAATCTCTGAAAGCAACAAGAAATTTAAAAGAATAAAATCTGACTTTTAACTGTTTATTATACCCCATGAGAACGAATTTAAATAAAATCCAGCGATGTCCAGGATGCGGAAACTTCCTCATTCACCTCGCGCTTAAACAAGCCCTTGCCGAGCTGAAAATCCCAACGCATAAAACCGTGATTGTAACTGGGATCGGCTGTAATAGTAAAATGAGCCAATATATGGAAGGCTACGGTGCAGAAACCCTGCATGGAAGAGGAATCCCTTTTGCAACAGGGGTAAAGCTCGCAAACCCTGATCTGACTGTGATTTCGGTTTCTGGAGATGGCGATAGTTATGGGATCGGACTTGGGCATCTGCTGCATGCTGCGAGGAGGAATCTGCCTTTTGTGCATATTACCTGTGATAATGAAAACTATGCTCTCACCACAGGACAAGCTTCTGCGACAACTCCCCTTTGAGTGAAAACCAAGTCTACGCCTGAGGGAAATCCTGTTCCTCCGCTCCATCCCGTGCATTTGGTGGAAACGGCGGGTTGTAGCTTTGTAAAATCGGTCATTGATAAAGACATGAAAACACTGAAAGAAACGATCGTTCAAGCGATTCAGCATTCAGGTTTTGCGCATATCAATGTTCAGCAAGCTTGTCCAAGCTGGAAAAGATGGTAGTTTTATCTCGTAGAAGAGCAAATATGGAAATGACTTCTCTTTCTTCAGAGTTTTTCTTGATACCTGATATCAGTGAATCAGATTTTGATTTTATTCAAAAAATAATAGCATCAACAGATCTTTCCTATTATGAAATCCAAAAGCCACAGTTTATCGTCATAAAAAATAAAGAGGAAATCATTGCTTTTTGAAGAATATTTCCAATTTGAGAAAATGAGGAAGAACTAGGAACCTTATGGGTCAAAGAAGCAATGAGAGGCAAAAAACTTTGACAAGCTTTGATAAAAGAGTTAATTAAAAAGAAGAGAAATAAGCCTCACCTTTATCTTGCATGCCAAAAAGGACTTCAAAAGTACTATGAAAATGCTGGATTTCAAAAAACCGATGTCATTCCCGAAAAACTCTATTTCACTCAGCAATGGGCAGAGGAAAACGGCTATTCATTTATCGTAATGCAATATTAGATTATTAACAGAAGAGTATACATTTTAATGACTATTAAATACCATGTCACAACTTCTTCAAATTCTTACAGCGCTCGGATCAGGGATTCTGATGGGAGCAATCTTTACCCTTCTTAAACTTCCACTTCCTGCTCCAAATGCACGAGCTGGAATTGCAGGTATCGCAGGGATTTTTATCGGCTCTTTGCTTGTCGGGCTTTTTATCAAGAAATAGCACATGAAACTCATTATTCAATCGGTCAGTTCCGCACAGGTAGAAGTCTATAGCGATGAAAGCTACTCCGAACGCATAAAAACAGAAAAAATCTGATCCTGAATTTTGATCTATTTTGGCGTAGGGAAAACTGATGAAATGCGTGAAGATCGAAAGTCAGCACTAGAGAAATTCACGACCAAGATCCAAACACTCAAACTCCTCTCTTCTCCGAACGGGAAAATTGAAATTCCGCTTGCTGAAGGAGGAAATCAGATTTTGGTGATTTCAAATTTCACACTCTTTGGAAGCTATAAAAACGGTACGAAAATTGATTTTTCTCAGAGCGGGAAATTTAGTTTTTCTGAGGGAGCGTATGATTATTTTCTGGAAAGTCTACGTCGTAAAGGTTTTAGCGTAAAGAGTGGGAAATTCTGAGGAGACATGCTCGTCTCGAGCATCAATATCGGTCCTATCAATTATATCTTTGAATTGTAGCGCTCTGCCTCGCTACAAGCAATGGGAAAAACATCTTAAATGATCTTCAATTCCCTCAAAAGTTTTTCATATTTTGGATAGTCCTCGTTATATTGGCTATGGAGGAAGGTTTTTAGTAAGTCCTTCCAGCCACCGAAGGTTTGCAAAGCGTCGCTGTCAGGTTTTTGGAGCTGGAGCAGCAAATCATCAACCTTTGCAGAAGTTTTTTTGAGTCTAAAGTCTGAAAGCTGAGTCCTGAGTTCAGGCGCAAGTGCTTCGTCAATTTTTTCATAGTCCAAATGCTTGACCTTGAGGGTAAGGACGGTTTTTTTTGGATCTAAAGGAGGGAAATGACAGTCGATCAACGGAAAGGTCGGAGGATTCAGATTTTGTTCTGTTTGGGACTGGATCTGGAGGTAGAGCGTTTGAAGATCGGACGCTTGGAGGGTGGAAGTAGGCTCGCTCTCTAGATAATTGATAATCTGCGTTCAGAAAAACGAAAACTTCTCCTGCGAATAGCTAAAAAATCCCTTAATCTGATTGAATTCTAGCGGAGAGGTCGCCCAAGCACTCCCAGTACAGAGATAATTTTTGTAGCGGAAGGCGGTATGCAAATGCCCAGAAATCAACTTCAAATCCGGAAGGTTTAGAAGTTGCTCGGATAGTGCAATATCTTTGAATGCAAAGGCAGAGCGATAGCCAGGAAATTTCTCTTTGTTGAGATAGTAATGATGAATAATAGTCAGCTTTTTTTCCTGTTGAAGAAAGCCATTGACGAGCTGATTGAGTTTTCCAGAGAATTGCTCGTTTTTGTCTTTGGATTTTAAGAGTTCCTCGGTGAGTGGCGTCTTTCAGAGTGGAAAAGCTGAATATTCTTCTTCGTGCAGATCCAAACAGAACGGAAGAAACAAAATTTTCTCGCCCTCAAGCTCAGTAAGCCGAGGTTTCGTGATGAAATGGAGCTTTCCTGAGTTTTGCTGGTGCTGGAGGAGATCAAACACCTGCTTCCCCTCTTCATACACGAAAGAGTTGCCCAGCCGATCGTGGTTTCCTGCGAGAATGTAGAGATTTTTGCCCTGTGTATAAAGTTCTAGAAAGAGTTCAAAGAGTGCGAGCAAGGCTGCCCTATCATAGCTGAAATGATAGACAAAATCCCCAAGAAAAATCAGATTTTTTTCTTCTGGCTGTGAGGCAACAAAAGCTTTGATCTGATCGAGCAATTCCGTTTTGATTTTTGAAGTGATGTGGAGGTCTCAGATGAGGAGCATTTGGGCTTAAAGAAAGAAAAGATAAAAGTCTGAATGGTTTTCAACGCTTCTTGTTGTAAAGAATTGAGAGCCTTTTGCAAATTAAAACAGAGGGAAAGTGCATGGCAGCACAAGAGGCGAGCTTTGAGGTTTTGAATGGTGAAACTCTCCCCTATAATTTGGAGTAAAATGATGTTTTACTCCCTTGCCATTTTGGGAATTGAGCTAGATTAAGCGGTTGCGTGAAATTTTGAGTGGGACCTTGTGGAAAAAGACGACATGGAAGCCGTCTTCAGATTTTTTTGACTTTTAAAAAATTATTCGCTTGCAGGTGATTGAAGGTTTGCTTTGAGTTGTTGAGTAGTTGCTGGATTGAGCTGAAAATCTGCCATTAGCGATCAGTTTTCGAAGATGAACGGTCTGGAACCTTCTCCAGAATAATCGACGTCGTATTTCCCCATTAAATAACCATTGAGTTCTGCTCTATTTTTGAATTTAACCGCTTGGTAGGGTTGATCAAAAGCTATTTTTTCGATAAACAGAAGTTCGCCGTTTTCGGAAGGCACGAGAACTCCAATATGACCGATGAAAAGGTTTGCCGTGCTTGGATCAATCACATCGTGGAAAAATACTGAGATCAAGCTTGCTTTTGTATTGAGGAACTCAACGCCTTTTTTAGCGAGCGTATCTTTTACTTTTTGGATATTTTCTGTTTGGGTTGCTGTAGCGGTTGTCGGGATTTGAGCGAAAAGGTTTACAAACTTATTAGCCTTTTCTTTTGTGTAGGACTGTATGGAGCGAAACCCAGGCATATCAAACATTAGATTTTCAGGGATTTCGGTCTCTGGGACGTCTTTTATCTTGATCGCGTCTTGTAAAAGCGAGAATGCTGTGATTCTACAATTGTAGCCCATAAAATCTTGGTGTATGTTATTTCGTTGCTGCTGATAAAAGTCGCTATCATACTCTGGCGTCAAAGTTTCTGCCCATGTAAATCCATGATTGAGTCCTTTTCACTCTACAATCTTGGTAAAATCATCTACCATCGCAAAAAAAGCTTGGACCGCTGTTTGGCTCACATCAGCCTTGAGGAGTGCGTTTTTGACCTCTGCTTGAGATTGAGCATCCGTCATATTTGAGTATCGGATTCCTGTGAGCTGAACATAGGATTGGGTTCAGGTTTCGGTAGCTATGATTCAGCTTTGTGTTTGAGTAGTATCGGTCGTAGTGGCTGCATGGTCAATTTGAATGCTGCATCCTGCGATGATGATTCAGAGAGAGCAGAGAAGTGCGAGTTTTTTCATCATGTTAGTTGAAGTAATTTGAATAAAAGTCGTTCACAATCTCTCAGTAACCTCTCTTGTTTTCAGGGAAGGTTACCTTTTGAGGACTGCTTTTGTAAATTTTTGAAGTTGTTTTGCAAGCTGATATTCACAGACAAAAGCATTCCAAGCTCCATCTTCACTCAACACTCCATGTGGCATATCTTCGGGAAGTTCTCCTCTATTACTCGCCTCAGCATCTCTAAACCGAGCTTTACTTTCGTAATACTTGAGCAATTCTTTGATCTGCGGTCTTAGGGCTTTTCGCTTCTCAAATGCAACATTGACTTCTTCCAAAGTTTGATTCATTTCATTGAGGATGGCTTCCATCTTCTGGATTTTTTGAAGCTGTCTTTGATGCATTTTGAGGTAAAAGCAGGGTAAAAGTCTGATTTTATTTTTTGATAAGGAAAAATGATTTTAGTTTTAAAAACCGCGCAGCAGATTCATAAGCCAAAGCTTCCAAGTTCCTATCTTTTGATGTAGTGATCCTTTTTCAGAGGGAGCTTGAAGAGTATTTTTTATTCTGTCTCCATACATTACCCTATTCCAACCTTCATCAATTTCTTTGCTAGAAACTTTTATTTCCCCTTCAGAGGTTTTGATAGTAATCATACGATCCTTGTTTATTTCGGGACTATTCATTTTCTTTTACATTCATTCTAAAAATTTATCTAATCAGTTCCTCCTATGGAGTCCTTTTGCAAGCCAATATTCGCAGGTGAACGCGTTCCAAGCTCCATCTTCACTCAACACCCCATGTAGCATATCTTCGGGAAGTTCTCCTCTATTACTCGCCTCAGTATCTCTAAACCGAGCTTTACTTTCCTAATACTTGAGCAATTCTTTGATCTGCAGTAATTTCTTTTTGATTAGCAGACTCGAGGCCTCTTCAGGAAGTGTATCACAAGCTTTTCGTCCAAATTCAGCGACTTCTTCTCTCTGTAAGGTCAGCTTTTGCTCTCCTGTCAGCACAAAGACAAACCTGAAATCATGGTGAAAGTGAGCGGGTTCTTGTTTCTTTTCGCTATAAGGAATGGCGTGAGTATCAATATCCAGAGGGATACCATGATGCTCTTTATGCCAATTATGTAAAGCAACGTTTTGGAGCCCTGTTTCCTCTATCAATTCTCTTTGTGCTGTGTGAAAGAGTTCTCCATCGCCTTCTTCCCAGTGTCCTCATGGAGCAAGCCGTTTATCTAGAGCAATATGATGAATCATCAAACACTTCTCTTCAGAAAGCAGATATGCACTTGCAGTCATATGTCCATTGAAGTTTTTTCTTGTTCCGAGATTTTCTGCGTTCTGGAGCTGAGTTTTTAAGAGAGAAAATCTGGATTCTTCTGAAGGGAAGGTATGAAGATAGCGTTTTAAGGTTTCCTCCACGTATGCAGACAGATCAACGCGAAAGGTTCAGGTTTTCTCTTGTTGCATTTTTTATCGTGAAGGAAAGAATAAAAAAAGGAATAGATTTTTGGCTGAAAATAAAGTGCTTCCTTATCAAAGATTATCCAATCAGTTCCTCTTATCGTCGTAAGTTAAAAAATGAGGCTCTGTTTTAGGCTTTTTATGTTTAGGGAAATAAGATACTTTTTCAAGTCTAAAAGGTTGACCTGTTTTCAAGACCTCACTTCTATAGTCAGAGCAATTTCTTGTAAAAGACATGGCGTTTCCAAAAATTTCTCCACCGCGAGGGAAAAAAACAGAGAATCAATCAGATTATTTTCCAAAAATTTTTTAGTCTTTTCATCAATAATGAGCAAAAGATGGGATGAATATTCAAAAAATTCACAAAATACTGGAGATTCATATCACTTTCCTAAGTAATTGTAGGAATACTCTCCCCATACTGCCATGATCCAATCAAGCAAATAGCATAAAGTATATGCTGAAGCTGAATCCTTAAAAATTTTTGTAAAATACTGATCTCACCTTACTTTATAGCTTCTTGATCATGGTATTTGTAAGATTCAAAAATGCACTTGCTCTGAAATATGGTCAGGTCTCTTTAAAATAAGAAAATCATCATCCGCAATTCGGATATTCTCTTTAGTATAATATTTTTGATGTTCAAGCTGAAGTATTTTTTTAGAAATCTGAGTCCCAAATTGGGCTCGTCAAAACTTTTTTCAATATATTTCCTTCCCAATACGATTTACGAGAAGAGTTGTATCTGAAATCTGTCCAGATAGCTCTTCTTTGATCATTTTTATTTCTCTCTTCATAATAGACATATCTAAAGGAGAAACAAACTGATCTAAAACTGTTTCTACCTTAATCGTTGGAGGCAAAAAGTAACTTGAGTAAGTAAGCCAACTCGTTCCAAGGACGCCGTATGCTGTTTTAAAATACTGCTCAAGATTTCAATATCCGCCGATAACTATATGCTCTATCAGGTGTTGGAGATAGCTATTTTGCTTGTAAGGTGCTGTTTTGATATAGATTCGCATTGTCTGATACTGCAAAAAGTAAAAGGCTGAATGACTCCATACTCTGCCACTTTAAAAACATTATTCCCCCTTGTCTAACAGTTTATCGCCAATTTTTTAAACTAACGTGAGCAATTCAAGTTAGGAATATTATCTAGAAATAAAGAATAAGATATTTTGTAAAAGCGTAATCGGATAGCGGGAAGCAAATCAAACGACACTCCAGAAAGATCAATCACTATTCATCATACATCGGACAATTTTCCAATGAGATCAAGAACATCCCATAACCTGTGAGAGAGATTCTGAGGATGAAGTAGCTATTACCTTAGCAAGCAAATAATAGGTATAGCAAGTTCAAATTAGTCCAACAAGGGTATAAACCAGAAAAAGAAGGAGTATTATTTTTACTCACCTGATCGAAACATCACTCATAGCAAAAAAATCTCAACATAAAATCTATTATCTAATTATTTCTTTCTCTATATAAGCTTGATATCCTTTGAGACTCTAATCCAGATTTTTTAAGTTCTGAATGTCATTTCCCAGGATGAAGTGTAAATTTTTTGTAATCTTCTCAATATTCCAATCTCGTCGCCTAATTTCCTCAAGTGCTTCGATCGTTTCCTGGTCAAACCTATAACGAATAAGTTGTGCAGGGTTTCATCAGACAATTGCATAGGGGTCCACATCTTTGGTAACTGTCGCATTTGCTCAAATAATTGCCCCATTTCAGATTTTTACACCTGGTAAGATCGTTGCATTGTAGCCAATCCAGGCATCATTTCAAACGATGGTATCTCATTTATTTACTCATTGTACGAGCTGCAAAGTTTTTGTCCAGTCTCAATTTTGAGAACTTTGGAGAGCTTTCATCCAGTCTCCTCCAAAAATTCCAAAAGGATAACTAGAAAAACCATCAATCTTGTGATTTGCTGCGTTCATAATGAATTTCACTCCAGTTGCAATTGCACAAAACTTTCCAATAATAAGTTTACTATCTATCATGGGGCTACGATACAGAACATTTTTCTCAAAATTCTCAATACCTTCTGGATCGTCATAATAGGTATAATCACCGATAATGATATTGGGATCCTGAATAAAATTTTTCAAAAAACCTAGCCCTTGGTATGCTTCAATTGGAAACTTTTCATTTGGGTTTGGTCAAGTCATAGCAATATAGAGAAAAGATAAAAATTGTGCACTTGTTCTTTTCAGACCTGTTTAATAAGTTAGCTATTCTAATAGACACTACGTCCTATCCAATGACTTCCTTCCCCATATATGGCTGCAAAACTTTTGGAATTCTGATCTTTCCATCAGCAGTCTGATTATTTTCTATAATTGCAATCAACACTCTTGGTGTCGCAATCGCCGTATTATTCATTGTGTAGCAATACTGGATTTTCCCTTCTTTATCGCGATATTTGAGATTCAGTCTTCTTGCTTGAAAATCCAGAAAAGCGGTCACTGAATGTGTCTCTCCATAAGCATTTCTAGAAGGCATTCGGCATTCAAGGTCATGAGAATTATACTTTCCAATCGCCAAATCTCCACTGCAAAGCTGAAGCAAACGATGAGGAAGCTCCAAATCCGCGAGAATATCCATAGAATTTTGCAAAATCTGACGATAATACTGCTCAGACATTTTCTCATCAGCAGGCAAAACTACGACTTGCTCTACCTTTTGGAATTGATGCACTCTATAGAGTCCTGCTGTATCTTTCCCATAGGTTCCTGCCTCACGGCGATAACACGCTGAATATCCACAGTATTTTTTTGGTAAATCATCTTCGTTGAGGATTTCATTCATATGATAAGCCGTCACTGGAATCTCTGAAGTCCCAATCAATCGCTGATCATCTCTCTCCATTCGATATGCGTCTTCTTCTCCTCCTGGAAAGTAACCGGTTCCCTGTAAACATTCAGGATTCACAATATTTGGCACCTGCATAGGAGTAAAACCCCTCTCTACGATCTTTTTGAGAGTATATTGCAAAACAGCCTGCTCAAGCAGCATTCCATCTCCCTTGAGAAAATAGCTTCTCGCACCTCCAAGCTTCACTCCTCTCTCAATATCAATCATATCGTGCTTTTTCATCAGTTCAACATGATCCAAAGGCTGAAAATCAAACTGCGGAATCGTTCAAAAATTCTGAACCACCACATTCTCACTATCATCTTTCCCCTCTGGAACCTCAGGATGCAAAGCGGTATTTGGCATTTTGAGGAGCCAGCTTTTAAGTTCAGTATCTACCTGCGTATGTTGAGTTTCCAACTCTTGAATCTGAATTTTGAGGTTTTTTGCACCTTCATAATCCTTTTGAGCAGCGAGTTGCTTTTGCTGAGCCTTGGTTTGATCAATTTCAAGCTGGAGAGCTTTCCTTTTATCATCTAAAGCAAGAATCGCATCAATATCAAAATGCTTTCCTTTCTTCTGGCAAATCAGCTTATATCCTGCAATATCTTCACGAACTTTCTTAAGATCAATCATTATTTTGAATAAAAGTAAAAAAAACTGACTTTAGTGTAGTCTTTTGCTTGTTAACTTCAACGAAAAAAAGAAAAAATCTGACCGTAAAGATCAGACTTCCTCTTTTTTCTTTTTTAGTCTATTGCATAGGTGTGATAGGTATTAACTCTTACAAAGACTCAGGAGTTTTCAGACGCCCCTTCTTCATCTTCAAAAAAACTTCTATTGGCTACACCATATTCACGATACTCTGGAGCAAATCCGTTTTCTGAGTAAACGATAAGTTTTCCGAGTTTAACTCAAAGCTGGCTTGCAACTTCTTTTGCATGCGTTTCTGCATCTTTATTCGCTTCGCTGATGAGTTCCTTCACTTGAGGATTGTTTTCTCCGACAATGAGCCTCTTATCTTCAATGTCTATCCAATCTAAGGCATCAGTTTTTTCTATGAGTGTTTTCAGCTTTTCGCTGATATTTCCGGTGAATTGCAAATTGACACTCATCCTCGTGCAAGGATATTCTTTTTCCGTTCCTCAAAAATAACATTGATCAGTATCAAAAACACGTCTTCCGTCATAATTACTATTGGTAAAGCCCGCTCCTTCTACAAAGTTTTTTACTTCTTCTAGTCTCTTAAAAGCCAGTTCCTTTCTAATAGCTTTTGTAGTTCCGGTTCCAATCAGTGTAATCCTAAACTGAACGGTATCTGCTGGGATTTCTCTGGATGCTACACCCATAATGTTGATGCCATTCAGATTACGATTTTTGGAGAAATCGTGCATACTCCACATTCCTGCAAGGCAAAGGATAACAGCAACCCCTGCAATAAATCGTCTGTCAGTCGCTTTCATCTTTCGTAGGGTAAAAAAATAAATGCTCGTCTATGATATACTCTTCTTGATAAAAATATCAAGCTTAAAAATCTGACCTTTCTCCTTCTTTTTGTTGCATCCTCGGAAGGAAGAAGGTCTCCAAACGAAACTTACAAAAAAATTGCCAAAAATGGATCAAAATTTCACCAAAAAGCGCAAAGCACTATCAGAACATCATAATATTTCATCAAAAGGCTCAAAGTAGTGTCAGAACATCATAATATTTCACCAAAAAGCTCAAAGCAGTGCCAGAACATCATAATATTTCACCAAAAAGCCCAAAGTAGTGTCAGAATATCCTATTCTTTCATCAAAAAACTCAAAGTGCTATCAGAATATCCTATTCTTTCATCAAAAAACTCAAAGTGCTATCAGAATATCGTATTTTTTTGACTGAAAAAAAACTTGACTTTAAGCTTCAATAGGATATGCTCCTTGAAAGCTTTATTTAGTTAGTATGTATTTTTAGCAATGAAACGTATTTGATTTCTCTGTTTGTCATTTGGATTTTCTCTACTCCTTACTGGTTGCTGGCTCAAGACTGGAAATCCTTCTCAAGAACCACAACCTGAAATAGTAAAGCAGGAGCTTCCTGATCAGGAGATGTCTCCACTCCTTTCTAGTACATCGCTCGGTAACTATGAAGAGATTCTTGAAGAAGTAGTTAATTCTGAAGCTCCTTGCAGTGTTTGTGAAATTAAATCAACTCCTGAGGAGATCAAAACTCTTATTATGCAAGCAAAACAGAAGGAGCAAAACCTTCCACATCCTGAAACCGCATCAGCTGAAACCTATTCAGAATTTGATTTTTTGAAAGCTTATGATACCGTTTATAGTGGGAACACCTATCGTATTATACCAGCTGCAACAGGGAAGAACCTGCTAACAGTAACTCTCAATGATCAACCTGTTTTTAGCTTTGAAAGGATCTTTATTACCAAGGAACCTGTTAAGAATTTTTTCGTCAATGAAGCTTGATGGTGGCTCCTCTATGATGAAAACTCTTATTATGATGAAAGTAATCCCCAAGATAAACAGGAAATGATGGCAAAACAAGGGAAACCCTGGAGAGAGACTAATGTTTTAGTCCATAATGGAGAAAAACAGACTCAAAATGCTGATAATTTTGCTCTTTATAATTTTAATGGGAAAACGTTCTATTTTTTGAGGAAGCAGGCTGGGGATAAGCTAAGTTATTTCTACGATGGGAAGGTTTATCAGACCGATTTTGATGAAATCATAGAAGATACTATGGGTTCAGATCTTGAAAATGCGTTCGAACCGATTGTGGACGAAGAAAAGGGATTCTTGCTCTTTTGGGCCAAAAAGGGTGGAGAACTCAGTGTTAACCTCTTGAAAACTAAAACTCTTGCGAAGATGCCATAAAAGCCTGAGTTTTTCGCTACCTCTCTATAAAGGGCTAAAAGAAACTTATGAAAAGACTTTGACAAACAAGGATTCTGATCTTCGTTCCTATGGTTTTCGCTTAGAAGCTAGGATGAAGGCTATCTTGTGTTTGCACCAGTCGAAGGATATCGTAATCACCAAGTCATGCCCCAGAGAATTTTTGTAGAAAAAGTTCATGAATGCTGAAAGCGTATAATATTGCACTTGATGAATATCACAAATTGGAAGATATCAACCGAACAACGCTAGAGTTGATTAAAAAGAGAGCTCTAAGCAGTAAAGCTCTTAGTTTGTAGCAGAAAGAAGCCGATTAAGGTAGGCTGCATCCACTGCGTCTGGAGCCAAAACGATCAGTTCTGGAATCTCGTAAGGATGCAGTTTTTTGATAAAAGGAAGCAATTCTGACTCTTTTTCTTTTGGAAATTTTATAAAAATCAGTTTTTCTTCGTCTTTTTTGATTTTTCCTTCTCGGAGATAGTAGGATTTTGCGTAGTTCATTACGTTTGCACAGGCGATCAGCTTCTTTTTGAGTAAGGCGGTGATGAGTTGCTTGAGCTTTCTTCCGTCATTTGGATAAGTCATCAGTAGGATTTTCATACTCTTTATCTAGAAACTAAAGTTCAGAACCCCCTCGCTCAACGACCGTAAATCCCTTTCTCTCAAAAGGTTCAAAAGTCTGAGCTTTCACTTCAATCGGTTCTTGTAAGGCTTTGAAGACCATAAAGACTCTAAGCAGCGAATCCTCTTTTGGAGTGATCGTCAAAGGAGCAGTCTTGGTATAGCTTTCTCCTGCAAAATGGATCAGGTTATAAGGGTTATCTTTCATCTTTGGGTATCGATACACGATGAATTCGTTATATTCCTTTGGAGTGAGTCAGATTTCTGCTAATTTTTGCTGAAGAAATGCCCTTGTTTCGCTTCCTTTTACTACGAAGCCTTTGGTGAGGTCATAGTCAGACTTTTGTGCTGGTTTGCCTTCTCGGAAAAGATAGCTATATTCCTTACCATCTGTTTTATTGATCAAAGTCCCCTCTGGAGAAGCGATTACTTCCCAGCCCCTGAGCTTCTCATCATAGGCAGGATAATCAGCGATAAGTTCTCCCTGATAGTCAAGCTGAACTTTGATTTCTTGCTTATGCTCCGGATAGAGGTAAATTACGGGCTTATAAGCCACAGGAGGATCCATTCTCTTTTTTATTTTGGATTGATAAAGATAATAGCCTCAGATCGCAACTCCCGCCACCAAAATCAGGCCTCCCAGAATTGCGAGAGCATAGCATAGATTTTTTCCCATTTTTTTGTGCTTAAGAAGCTAAAACTGAGCGATTGCGAACTTTTTCCCTTTCTTTAGGAGTAAAACATTATTTACGGCATCAGCTTTTGAAAAGTTTTGTGCAATATCGCTTACTTTCTGCTCATTGACTGAGATCGCTCCCTGTTGGATCAGTTTTTTCGCTTCTCCATTGGATTCTGCGAGTCCAGATTGTACCAAAAGCTCTAAGATTCTGATTTCATCATTTTCAAGCTTAGTTCCTCAGGTTTCGTGAGCAAGAGCTTTGATTTCCTCCGCAGACATTGCCTTGAGTAAGGCGAGCTTTTCTCCAGTTCCAAAAAGGAATTCTGAAATCTTTTCCGCCTGTTCACTTGCCTCTTTTCCGAAGAGCACTTGAATTACATACTTTGCAAGCTGTTTCTGACCGTATCTGAGTTCAGGTTTCTCCTGATGCTGTGCAATAATCGCCTCAATCTCTGGAATCTCTAGCAAAGTAAAGGCTCTCAAATACTTTTCCACATCAGCATCTGCGGTATTGATGAAGTATTGGTAGACGAAATAAGGAGAATTTTTGGTCTGATCAAGCCAAATCGCGTTCCCTTCAGATTTTCCAAACTTTTTCCCGCTGGAATCCATCATCAAAGGGATCGTCATCACATAACTTTCGGCTTCTCCTTCGGTTTTTTTCCTCAGCATTTCGGTTCCGGTAACCATATTTCCTCGCTGGTCAGAACCTCCGATTTGAAGCTTTACACCGTTGTTTTCGTAGAGATGCACGAAATCATTTCCCTGCAACAGCATATAGCTAAACTCGGTATAGCTAATCCCAGATTCAGGGTTCTCCATTCTCTTTTTCACAGATTCTTTGCTGAGCATGGTATTGATCGTGATATATTTTCCGACCGTGCGATACCAGTCAAAAACCGTATAATCCTTGTAAAAATCGTAATTATTCACGACTTCAAACTGAAAGTCCTTTCCTGTCAGTTTTTTTAAATTTTCCAAAATCTGACCGACTTGCACGGTAATTGCTTCTTGATTATGCCTGAGTTCTTCAAGCCCAAGGAAAGCTCTGGCTTCAGTTTTCCCTGTTGGATCGCCGATCATTCAAGTCGCACCTCCGATCAGCATCACGAAGGTATTGCCTCTTTTCATAAAATTCACAGCAGCCATAAAGGTCAAGAAATGCCCTAGATGAAGCGAATCTGCCGTAGGATCATAACCACAATAGAACTTTTCACCGCCCTTCTCATACAAATCAAAAAAGTTTTCGTTGGTCATTTGGTAAAGTAAGCCACGGGCTTCAAGTTCTTGTCTAAGATTCATTTCTTGAATAGGAAAAATAAAACTGAGTCTAGTGTAGTATTTATGCTTTGATTTTCAAGAGATAAAATGAGTTATAGAAGTCAGAGTTGTCCAAGCTTATGTTTAATTTCTTGGTGAATTTCTTGTGCAGGACGTTCTCAATCTCCATAAAGTAGCCAGAAATTTTTGCTCTCTCTAAAAGCTAATACACTATTGTTTGTCGTATGAGCTAACACTCTCGTGAATCTAAATATAAGGGAAATGAAGTCTTCCCCGAGTTTAAGCTACCACTAGTCTAAATAGCGTAAAAAATCTGATTTTTTGACTTTTGGTTTGGTTTTGCGTATACTTGAAGGGATTTAATTTACATAATGATCCTTATGGGAGAAAGGCTTGAAGAGAAGAGCAATCAGGACTCGATCCAGAAAATCGCTAAAGATGACCAGCTTGATCATAAACACCCACAAGTGAAGGAAAAGATTGCTGAGGATTTTTCTGGTTCTCAAAAAGAAGGTTTAGAAAAGCCATCTCTTAATTCGCTTGATAGCAAGCTGGCTGAGATTTTTCCCTGAAACGAAGCAATTGTATCTAAAGTGAAAGAGATTCTCATCAAACTAAAAGAATTTTCTCAGGATTTTCTCTCTGATCTTCTTACCGTAAAGAGTGGAAAATCTCTCGGGAAAGAAAGATTCTGGTCAGGCAAGCGAGAAGAACTTTGAAAACAAATACATATTGCGATCAACAAACTGAATACCGCTCGTAATGAAGGGGAATCTATGCAGGGGGAACAGCTTTTGGCAAAGGTTTCTGACCAGTGGACCTCGCTTCAGTTTGAGCAACTCAGAGGGGAAAGTGATCAGGCGAGGATTCAATGAAAGCTGAATCAACTCCTTGATCTTTATCAAAAAGGCAACCCACAACGCTCTAGAGAAGAGTTTAGTGAACAATACCGTCTCCTCGAGAAAAATGTCTGAGGACTAACTGCTGAATGATTTTTGGATTGGTACTTAGAAAAGCTCACTGCATCTTGAGAAAAAGATAAAAACCTCTATTCAGACTCCTTAGATGCACTATACTCCACGTGGAAAGATGGGGAAGTTATTCGTATTGGATTGGATAAAAACGGTCAGGTGAAGAGTTTTGAGGAGTGGGAAAAAATGCTCAAATGGTCTGAATCCAACAACGAAGAACTCAAGAAAAATGCTGAATCCATAAAGCGTGCAGTCCTTGAAAAAGGTGAACTTTCTGGAGAAGCACTTAAACAAGAGTTTGCAAAAATTGATGCTGCGTGGCAAAAAGAAGCAAAAAACGCCTATGGAATGCTTAATCGTAGCGAAGTGATAAGATCAGCTTTTGTCTGATTAGAAAATCCTAAGGATTATACTACAAAAGACCTCATTAAAAAGATTCAGGCAATTGGACAGGTATGTTATCCTCGCTCTAATATCCAGGTCAATGGAGTATGGTCAGCAGAAACTCAAGCTATTGTGATGGATCTAAAAATTCACCCTCTCCTCAAAGATGCTGAAGAGTGGCTTGATGCGGTTCTTAGGGCGAATAGAGACTATGCAGCTACAGCAAAAAGAGCTTCTGAACGCCTTGCAAAAGCTTACGAAAAAGGCAAATATGAAGGAGGAGAAGCTCAAGTTATGGAAAGATTTTCTACGGGGATGATGCGTCCTCAAGTGAATGCATCTCCAGAAAAGCCATCTCCAGAAAAAGTTAAATTAGAGAGTGCCCAAGATATTGGGATACAACGTAACGCTTCCTTACAGCAAGCCCTCGCGTCTATTAGTGATGAAGATGTTGAATCTACGATTCCGAGAGCAACCTATCTCCAAGAGATAGCGGCGTTTATTCAAAGAGATAGTAGCCTTTCTGTTGAAGATAAAATTCGTCTTCCAGAACTTATTTTGGAGGGAAGTTGATCGGGGGAGGTTTATAAATGACTAAAAATGAAATGGGATGCAAGTCTCGCAAAAAAAAACTTCCTCGCATCACAGAAAGAGAGCCTTCAACAAAAAATCTTAAAAACTATGCGTCAAAAAGCGAAAGAGTCAAGAGATCATTTCTTTACTCATGTTGGGCTTCCTCTTAAGTACGATTCCCAGCTTAGTAAGGCTTATGAAACAAGATATAAAAAAACTCCAGGGACTTATTCAGTTTTTACTGATGCCAATAATCCTAATGATCAGTATGTTTTCAATCATCGCTCTTGATGCTTAAGTATGAAGAATACTACGAAATTTAACCAGCAACAAAAAGAGATCAACTTTAATGCGACGAAATTTAAGGCGATTCTCCGCTTTCCGACACCAGCAGAACAAGTTCAAAATGCACTGAATATTAGCTCCTACCTTCCAAGCTTAAGACCTTGAACTGAAAGAGATTTGGATACTTTACTTAATGATGAGCTTAGCAAAAAAATCTCTCACACGGCAGGTGATATCGAGACCTCAATTATTCATAAAAATATTGAATCTAATAGGCTTAACTCCTCTATTCTCAGGCACGCACAACGCCTTCTTAATCTCGAGTCTTCACAATGAAAACTCTCTGAACAATCCCATAAAGCACAGTATGAAATGCTTGTCCCACTCCTCAATACGCTTCCTCAATGTTCAATAGATGAACTAAGGACTGTTGATCGCTTTTTTTCGGAGTTAGAGGCGATGCTCAATGATAATTCAACACAGAAGGAGGGAACTCATGAAAAAAATGAATGAAATCAGATTTTTTCGCTCCTTTCTAAGCCTTGGATGAAGAAAAGCTTTAAAGAGCAAGGACCTCGCAAATCAAAAACGGATTTTTGAATAGGGCTTCTCTTCCAGTCTTTGGAAAAACCTCTCCCGGGAGCTACTGATGATCTAGAACATAGGATTTTGGATGTAAGGAAGATGAAGCTCCTTAGTTCCAACAACCTCTCTCATCTTCGTGCAGAACAGGCTAGCCAACAGCCTGATGGACAACGATATTCCAAGCTTACCAAAACAATTGCCAATAATTATATTAAGACCGCAGAAACAGAACAGCAACACGTAGAGCAACAGCAACTTGAAACCTTGGAAAAACTCATTTCTAACGGCTAAAATCAGACTTTTTATCTTGTAAACTCTTATCCACGAATGGAAATCTTTAGTGCAAAAATCATTCCTTTAAATGTTGCGAATGGAGACGGGATTATCGCAATCCTCAACGCAGAGCAGGCTTCTCAATATGGGATTACGCAGCATGATAAAATTTCACTCATCAGAAAGGGTGAAGAATATGTCGTAGATGTTGCTCTTTCTAGTGAATATGTAGAACCTTGAACCATCGGTGCGACAACGGAATTGCTTGAAAACTATCCTATTATGCCTTGAGATAATGTTCTCGTTTCTTTTATGAGAAATAATCCGCTTTCTTTGCAGGCAATCAGGAAAAAGCTGCTCGGAGAGAAAATCACGACCGAAGAAATCGATGCGATCGTGGAAGATATTCAGAATAATAAACTTTCTGATCTGATTTTGGCATATTATACAGCAACGAGCTTTTTTTATAAAACAGATCCTGAAGAACTGGCATATACGACGAAAGCGACTGCTTATACCGGAGATATGTACCGTTTTCCATGAATCGTAGCGAGCAAATACTGTATTGGAGGGGTTTCTGGGAACGAAACGACAATGATTTTGGTACCGCTCCTTGCTTCGCTCGGGATCGTAATGCCAAAGACCTTTTCTAAGTCGATTACTTCTCCGGCTGCGACCTGAGAATGTGTAGAAGTCTTGATGAGAAGTGAGTTAAAAAAATCTGAAATCATCAGGCTCGTAGAGAAATACGGATGCTGCCTCGCTCGAAATGGAAATTTGAACCTCGCACCGGCGAATGATAGAATCATCAAGGTCTCTGCTCCTCTGGGAATGGAACCGTATGCCAGGATGATTTCCAGTATCATGGCGAAGAATTACGCTATGGGAATTAACCACTGCCTGATTGATATTCCTATTGGACCGACCGCTAAAGTAAACAAACAGGAAGATGCCGAAAGAATTGCTCAACATTTCAAAAATATTGGAACCTCACTTGGAATTAAAACAGAAGTTGCGATAACCAAAGCTGAACAGCCGATTGGAAACGGGATTGGTGCGGTTTTGCAGGTAAGAGAAGTGTTGAGAGTGCTTCAGCAACACGAGTTAAGAGCTTCTGATCTGGAAGAGAAAGCATTGGATTTGGCGGCACAACTCCTCGTGCTTTGCGGAAAGAATGAAAAGTATGAAGAAGCGTATAGGCTTGCAAAGGAGACACTCCAGAACTGAGATGCACGAAAAAAGATGCAGGAAATTATTTCTGCACAAGAAGGGAATCCTGATGTAGATTCAGAAGGATTGGTCTTGGGAAGTCAGACTTTTGATATTGTGAGTGAACGCGATGGAAAAGTAGAAGCGATTGATATGAAATATCTCAATATGCTAGCAAAGACGCTCGGTGCTCCGTGAGATGCAAGTGCTGGGATTTTCTTGCATAAGAAGCTTCACGACAATGTGCAAAAGGGAGAGAAACTCTTTTCATTTTATGCGAGCTCGGAAAATAAGCTCCAAATGGCGAAGGAACTAGCGGAAGAAAAGTCTGCATATACGATCGTGTAAGCTTTGAAAATTACTATTTTTGATGGAAGCACTTTGGAAGAAGTGCTTTTTTTGTCTTTATCTTTGGAGGGAATTCTGTATACTGCGAGCTCGCTTTACATTGAAGGAAAGTTTTCTGATGCACATGATCTGATTTTTGTTTTTTTCTTTTGTGGCATTCTGAGGATTGGCAAGTCTGACAGTTTTGAGGATTTCGAGTTGGTGGGGACGAAGAATTGGTCGAATCCAACGAGGACTAATCGTGCTGCTCGCCCTTATGGTTCCTTTGGCAATGTGGATAGCAAGAGCCTTTCAGGGTATCGTGGCTGAAATGCTTGCAAAGGCTGGGCTCTTTTTGCTCGGTTTTTTGACGGTTTCGTTCAATGTGATTCTGGTTTTTGAACTGTTAAATTTTGTGAGTAAAACGATTTTTCATACCGATATGAGAGGCATCGTATGAGGAAGTTTGCTCATTATTGTGTTGATTGGGACCACCGGGTTTGCGATGCGGAGTGCAAGAGCGATCAAAGTCAAAACCGTCGAGATTCCGGTCACGAACCTGACGAAAGAAAGCACCATCCTCTATCTCTCGGATATTCATATCTCTCATACTAGTGATTTGCCGTTTTTGAAGAAAATTATTGCGCAACTGAACGCTTTGGAGGGAGAGTTTGTGGTGATTAATGGAGATTTTATTGATGGGAAGGGGTTTTCGGAAGCAGATCTTCAGCTCTTGAATAGTATCAACAAAGACGTCTATCTCACGCTTGGAAATCACGAAGCCTACGCAGGAAACGCATATGTAAAGCAACTTTTGAAAAATACAAAAATCAGACTCTTGGAGGACGAAGTGCTTCATGCCCACGGATGGGAACTGATTGGACTTGCTGATATGGCAGGATTTGATACTGAGGAGAACCATGAGCTTCTTGATCAAAAACTCGCTACCCTCCTCACTAACACGACAACACTCCCTAAACTCTTAATTCTTCACGAGCCGATTGGACCGCAAGTAGCAGAACAGCATGGGATCAATCTTCAACTGGCAGGGCACACTCATTATGGTCAGATTTTTCCTTTTACTCTCGCTGTAAAGATTGCGTTTCCTTATATTAAGGGGCTTTACCGTTTTGGTACGAATGCGTTGTTTGTGAGTTCTGGGATCTGAACGTGGGGTCCCACCATGAGACTTGGAAGTCGTTCAGAATTAGTAGTTTTAAAACTGATCCCAAAGGGCTAGTTGTCCTCCATCAATGATAAAAGATCAAAAATCAGAAGTAGACCACGCTTGTAGGGCTACCATCCCTATTTTCAGGACTGCAAGGAATGATCGTATGGCTACAATCGTTCCTTGCAAGCGTATAAAGGCGAGTTATAAACCTATGCCCTCCTAAGAAAAGGGGAGGAATAGAAAATACCCTTCGAAGAGTTTACTTATGGAGGAAAGAAGGTTTCTCATCCGATGTATAGGTATCAATTCCCACATAATATCCATAAAGAAGTGAAGGTGATGAAAATGAAATATCATAATATTTTTTGGGTTGATCAGAAAACTGGATATACATATGACTATTACATCCAAAAGTTTTTATCGTTTGTCATTCAATTCCCCTTTGAAGGTTCTGCCCTACCCCGAGAGTCCTTTTTTGCAAATGAGAGGTATCTCCCGTATATCAAAGCACTACCCCTGTTAAGGTTTTTCCTGCTTCTGCTAGCAGATAGGGATGATCCAATCAATAATATGCCATTCCTGACAAATTAAATCCCATCTCAGGAGAAGCTTTTTCTAGACTTCCGCATTCTTCTTGAACCTTCCCTTCTAGCGTCTCTCCCTCCCAAAGTAATCTTGGAGAAATATTGATGTGTAAGTCATTTCAAATCCCATCTATTGGAGAAGAAATATTAATATCTTCCGTTCTTCTTGGGAATCTTTGTAGTTGAATGAATTGATAGCATTGGGTATTGGCAAGCCCTTCTTCCATATCCATAATTTGTTTTTGATACTCTGGATTTTCTCTCTTCATTTTATCAATACTCGTTTTTACACTCTGTTTGAAAGTTTCACAATCTCAATAGAAACCTGATCCTGCATCTGCTTTCGGGAGGTAAGGCACCAAGAAAGAAAATCATAACGCTGGAGAAGAGACAACTGTATATTTGAATTGATCCACAGGAAAACGAGTGTTGATTTTTAGCAAAAGACCACTTGCATTCATTAGTCAAAAGCTAGAGAAACGTTTTTCATCTGTAAGTTTACCGAGTGAAGCAACCTCAAAGCCTCCTGTCAATACCTTCTCCCATTGTTTTTCAAGCTTTGGGAATTGTTCTTCTAGGAAGTTTTTACAGACTGCTTCAATAGCTTTATCTTGATGTGAACTTGCACATTGAGTAAAAAGAGATTTAAGCTGATCTAGAACCTCTTTTCAAGTTGTAGTCAGGGTTTGTTGTTTTAAAATTTGTGTTGTAAGGTGTTCTAAATATTGTGGACTTGGTGCAGCAAAGCTTATCCCTCGTCCCAGTAGTAAAGTGAAAAGAAAAATCAAAGGTTTTTTCATTGGAGGAAAGATTGAAGATAAAAAAGTTAACACTAGGATAATAATTCCCAGACGAACTAGTTGCTTTATTTGCATCCAATAATCATAAATAGTTAACAGTTTTTTAAGAATTGCAAGTAACAAAGTCATTCTCTAACTTCGGATTTCGATGCTTTATCAAGAACTCTACTTTCAAGTTCTTTTCTTTTTATACCATACGTTATTTTTTGTTATGCCAACTCAGATATAATTTTTCCCAAAATAATTGCAAGAAATATTTTTAGTAGTGGATATACTGTTTCTTGATTTTTATAGAGAGATATTTATATTCAATTCCATCTTTAGCTTTAAACATAAAAGTATGAAAAGGTATGCTGTAGGATTTCTTATTGTTGCTATGTTGCTCTTCAGTGGATGTGCAGTGAAGGAACAAACCATTCAAACAGGAGAAGTGCTTGAAACCTGAGAGACGACGCAAACCGTGGTTATCACAGAAGCAAATAGCTGAGATATCGTGACGCAAACGGGAGATAACCTTATTTATAGAAATGAAGTCTATGGATTCCAAGTAAAACTCGGGAAAGCACGGAAAGGGGCACAGATAGCTGGTGTATGGTGAGGGAAGCAGAATAGGTCTACAATATATTTCACCCTTCCTTATACCTATGTCCGAGAAATAGATGAAGAGGCTGTTAATCCAGATAATGTAAAAGGTATGAATCCAGATTCCATAACCGTCTTTAGAGGTAATATTTATCCTGCTGATGTTTACCAGGAGATTGGAGTTTCTAGATGCAAGTATCGAGATCCAGATCCATGTAAGACAGAGAAACAATCAAAAGAAGAAAGAGAAAGTCTAAGGGGAATCACTATAGGACATAATAATAATTTCTACTTTACAGCAAATAAGGATCTTACTCGGAAAATTGATGATCCTCATTATGAGTCACATTATAGCAAGAGAAAGGATGTCTTTGATACGATAGAGTTCTTTGATGTAAAGTAGCAAAAAATCTCTAGGAAAACCTAGAGATTCTTTTTTGCACAAGGTAGGGATATTTTTCCTAATACATAGGCATTCGTAACCACCCTTTTACTGTGTAGTTTTCAGGAGAAGAAATAGTCTTCGTCCGATAATCATAGTCCAACTTAGCACCAACGGGCTCATGCCAGTCAGTTTCAGGTTTCCCATCTTCGACAAGCCCCATATTTTGATGTACAAGCCTTACATAAGTATTTGTTACTTCCATGATGATAGCAACATGCCCATAGCCACCTGGTCCACCAGTAAAGCAGAGGATATCCCCAGGTCTTGGTGCAACTGATCCTGAATTAGGGAAGGCATCTAAGCCCTTTGCTGATGCTTCTCTATACCAATCTCTTCCATGCCCTCCATTTTTTCCTTTATTTACAATATTTTTCTTGTAAACTTGGGAATAATAGCGGTTGCAAAATTCAGTACATTGAACACTCAGCTTATTTGCATCATAAAGCCCTTTCCCAGCAGCCTTTACTACAACTCCATTAGCAGTTGCAAGTTCCTCCCCCTGAGTATACGAGCCTTTTGAATTGAATAAAGGATAAGTATATACAAGGATTGGTTCTGCATAATAGCGAACGCCAGTCTTCCCATCTATTACCATGGGATAGAAATGAACATATCCCGTGTTAAATGTTGCAGAGAACGAAAGTTTTATACTTTGTGAGAATGCCGAATAGGACTTTTGACTCGCAATCCCACCACAGGGAGACCCTGCTTTAATATAGGCAACTCCCATCCCCAGGAAGAAACCTCGATTCTGTTTGATAATCTCCACTTCAAAGGTTCCATTCACTTGTCGAGTAACTCGAGCCTTGATAACACCTCCTTCAGCAGTTCCACAAATCCCCTTTATCGGTAAATTCCGAGAACTGTTAGCCGTCCACACACCAACTCCGGGTGTCGAGTAGTAGGTTACATCACTCGCCAGAGTTGCCGCTCGTAGAGCAACCGAGCTATCAGGAGATGCTTGCTCTCCAGCTTGTTGATCCGAAGAAGTAACTACAACTTCTTCCACTTCTTGTTTCTCGCAGGAGCTCAAGAGAGCTACAACCACGAACATTCCGATAAGCATCAATACTGATGCAATCTTCTTTGCTTTCATTTTTTTTCCCTTTCTTTTTTTAGGAGTTATTATTTTTTTATTTTCACGAGAACGCTTATAATGTTTTAATAGACAATTGCAAGTATAATTGTCACTAAAAGCTGTCTGCAAGAATGAAGCCTCTGTGTGGAATTTTCAAAAATCGTCATTTTACTCCAAACTATAGAGTGTGCTTTCTCTTAGAACATCATTTTAGTGGGAACTACAAGGATGGGGGGGGGGAGTCAGACTTCCTCTTTATTTCTTTTCTTCTTGAAAAACTAGCGAGAATAACTAAAGTGAGGAGGAACTTATTTTTTACTTAATAAATACAACAGTCAATGAAACTGGATGGAAAAAGTATTCAGGCAGCAATTATGCATCTTGTGGAAGAATATAGATTAGATCCCTATCAAGTGATGGAAATTATTAGAGCAGGGATCAAATCAGGATTTAAAAAGGATTTTCCAGAATTTAGGAAAGCAGAAATCGTCGTTAATGTCGAAAACGATGGGACAATCAGTATTTATAAGGCTTTGACTGTTGTGGAAGAGATTGAGGAAGAAGACACCCAGATTCTTTTGGCTGATGCGAGGAAAGAAAGGAAAGATATTGAGCTCGGAGAACAGTTGCTAATCAATATTACTCCTCAGAGCATGGAACTTTCAAGGATTGCCGCACAAGCCGCTGCACAGACGATCAAACAAGGGATCAAAAATATTGAAAGAGAAAGGTTTTATGACAAGTTTCAGGATAAAGAAGGTCAGCTCTTGAAAGCAAAGGTGATCAAAGTGCATACAGATAGCGTAATCCTGGATATTGAAGGGACAACGGTGGTGCTTCCTCCAGAGAGCCAGATTCCTAATAGACCTTATGAAGTAGGAGAAGAAATTTTTGTTTTCCTCAAGAAAATTAGTAAAGAAGCCTGAAGTATTACCCTCGAAATCACCCAATCTAGTCCAGATTATGTAGAAGCAATCCTCAAAAAAATCGTTCCTGAGTTTGAAGAAGGAATCGTAACGATAGAAAAGATTGGAAGAGTTGCTGGAAAGAGGACCAAAATCTTGGTAGCTAGCACAGATGAGAGCATTGATCCAGTGGGAGTATTTGTCGGACATAAAGGAGACAGAATCAATACGGTACTTTCATTGCTTGATGGAGAAAAGGTTGATTTCGTAGAAGATATTGATGATCCAAAGGAATTGATCACAAGGCTTCTCAAACCTGCACAAGTAGAGAGTCTTGAGTTTAAGGACAACGGGAAAGTGACTGCGAGAGTAGAAGAATCACAGAAATCACTCGCTATCGGTAAAGGTGCCTGCAATATCAAGCTTGCTTCTCAGCTTAGCGGATATCAAATTGAAGTTATTTAAGAAAAAAGGAGGAAATCTGATTGTTGAGTATCGGGTTTTGTAGCATTTGGAGGAATTTTCTTTGAAAAGATTACTAGCGAGGGAATGCATAAAGCTTTACTCGCTAAGGGAAAGATGACCGCTCAATACTTTCAGACTTTTTCTTCTGGTTTAGAAACTAGCAGTGCTTTTGATCTCAATATTTGGTGCTTTTTTATCGAGAAACGCTTTATCTCTCTATCCCTATTATTTACGCTATGCTCAACGTTCATTCTATAGAAACTTTTGCGACGCAAGATGGTCCTGGGATCAGATTGGTGGTTTTTTTGCAAGGGTGTATGTTTAGATGCCAGTATTGTCATAATCCGGACACGATTCCGCTTGAGAACGAGAATGCAAAGCACCGAACCGCAGATGATATTCTCCTCCAAGCAGTAAAGCAAAGAGAATACTTTGGAGAGAAGGGCGGGATTACCTTTTCTGGAGGAGAACCGCTTACCCAAGCAGCAGCCTTACTGCCTGTAGTCCAAAAATTGAAAGCTGAAGGGTTCCACATTTGTATTGATACGAATGGATTTTGGCAGACTCCAGAGGCAAAAGAGGTTTTGAGCTTGGCAGATCTGATTTTGCCTGATTTGAAGCATATCAACCCAGAAAAACATCTCAAGCTCGTAGGACAGAGCAACGAAAATACGTTGAAGACACTGGATTATCTCGACGAGATCAAGAAACCTTACTGGCTCAGGTATGTCCTCGTGCCTGGCTATACTGATGACGAAGAGGACCTCCATACACTAGGGAAGTATCTACAGACCAGAACTGCTATGGAAAGGATTGAGATTTTGCCGTATCATAATCTGGGAAAATCCAAGTGGGACAAGCTTGGATGGAAATATCCTTTGGAAGGTGTGCATGCAGCAACAAGCCAGAATCTCGATACTGCGAAAAGGATCCTGAGTCAGTATTCAGAGAAAGTGTTTGTGAGAGGTTAAAGCTCTGGAGAGTGATTTTTTACTTTGTTTTTCGATTCCAACGATGAAAATGCAAGCCCTTGAAATCAGCGAACGTAGCTACCAAGAATTTACGAAGCTCAAAAAAATTATTGAAGCAATGGCAGGAGAAACCGTTTCTGATGATCAGGTTTTTGACTTTATGGTAAGGAGTTTATTGCAATCTATGGAGCTACCTGAAGAGGAGGGATATACCCATGGTGGTGGTTGTTGCTGCTGTGGAGGGCATGATTAGCCATAGTTTATAAATGCTGATTTTTATCTTCTTTTTGTGATGAAAGCACAAATCCTTACACTGAGGACCTGTAAAGAAGGGTTTCAGGACCTCTTGGAACATGGATGCCAGTATAAATACAGAGAAGCAAAGCCTTTTTGGAGAGCAAGGCTTTTTTCTAATGGGCAAGCAAAGCATTTTGACGAGGTACATATCAAGAATGGCTATCAACCGGATTCTCCGCTCGCAATTTATGAATTTTCTGGGATTGAAGGGCCTGAAGTGGTAGAAGGGGTGCCTTGCTTCAAGATTGTTTTGTGAAAATTGAAAGCAATCTATCATTCGCCTTCTTAGTAAAGATACCTTATCAAACTTTTTTTTGACAACAACGAAAGAATAACTTTCACTTGATTTTTTTGTATATAAAAGTATGGTTGGACTGATACTTACTTCTTTTTACTTGGGTATCATGATTTAGACTCCTTTTCTTCGTTGATGATTGCACGTATCAAAACTTTTAATACGCTCGGGCTTCTGGGGCAGGAAATTACCGTAGAGGCTGACAGTAGCAAAGCTCTCCCCACAATCGAAATCATTGGGCTTCCTGATCTGATGATCAAAGAAGCAAAAGAAAGAATAAGAGCCTGTTTTAGGAATGTGGGAATCGATCTTCCCAAGAGGAAATTCATTCTCAATCTCTCTCCCAGCGATCTCAAAAAGGTGGGAACCGCCTTTGACCTCCCTATGGCAGTTGCCTTACTCTGCCTCATTATGGAAGGTAAGGTTGCACACTATGATAGAATCCAGGAGTTTCTTTTCTTCTGAGAGCTAGGGCTGGACGGGAGCGTAAAGAGAGTAAATGGACTCCTTCCTAGCGTCATTTCTGCAAAAAAGAAAGGCTATCAAGTTTTTTTCGTGCCGGCAGAGAATAGCTATGAACTAGAATATATTTCGGACATTACCATTATTCCGCTCAGCCATTTTCAGCAGATCATTGACTTTCTCCTAAAGGGAACAGGGCTTTCTCACCTTCGTAATGCAAAAAATCTCGACGAGCTCAGCTCACAACAACAAAGCTTGGAATCTGATTTTGCCTATATTAAGGGGCAGCTCGTTGCAAAAAGAGCGCTCAGTATCGCAGCAGCAGGACTGCATAATCTCCTCATGGTCGGGGCTCCCGGTAGTGGAAAGACGCTTTTGAGTAAGGCACTGCCGACGATTCTTCCACCCTTGGATACTTCCGAAATCCTTGAAGTGAGTCAGATTTATTCTGTTGTAGGGAAGCTTAACAAAGACCTTCCTCTCGTGATCCAGAGACCCTTTAGACAAGTGCATCATACCGCTTCTAAGGTTTCTATTATTTGAGGCGGGAGCAATTTAACGCCTGGAGAGGTTTCTCTCGCGCACAAAGGGATTCTCTTTTTTGATGAGCTCCCGGAGTTTCCAAGAGAGGTGCTCGAAGTGCTTAGGCAACCATTAGAAGATGGGATCATTAATATCTCAAGAGTCAATGGAACCGTGCAGTATCCAGCAAATGTCATGTTTATCGCAGCGATGAACCCTTGTAAATGTGGCTACTACAAAGATCGCGAGAAGACTTGTTCCTGTAGCCTCAATGACATCAAAAGATATCAAAGCAAAATCTCTGGTCCCTTACTAGATAGGATCGATATGATCCTAGAAATCCCGAGGGAGAAGGTCGACTCCTTGCTCACACAGGGGCAGCATCAATCTTCAGCCGAACTCCGCAAACAAGTTATCAAAGCTCGAGAACTCCAGAACGAGCGTTTCAGAGGAAGCTGAATTCAGACTAATGCCATGATGACAGCAAAAGATCTTGATTATTTTATTCAGCTTGATAGCGAGAGTAAAGATTTTCTCAGGCAAGCGGCTGAAAAGCTTACGCTTTCTGGGAGAGTGATTCATAGATTACTGAAATTGGGGAGAACTATCGCGGATATGGAAGGGAATCCTGATCTCAAAGTCGGACATCTCATGGAAGCGCTCCATTATAGGAGTAAAACGATGTTCGTTGAAGCGGCATAAAGTCTACCTTAAAAATTCCTTGTATTAGCGCTTAAATTGCTTATGCTTGAGCTCGTGTTTTAGTTTGTGATCAAGATTATGGAAACTATTATCATTTGTGCTAGTCATGAAAATTTTGTCCCAGAAAGGAAGACTCCGGAGGCGATGTGCTGGGATATTAAAGCAGCAGAAAGCCTGAGTATTGCTCCTGGAGAAGTCAAACTCGTCAAAAGTGGAATAAAAACCTTTATCCCTACCGGACGATGCTGTAAAATTTATGCGAGGTCCTCGCTCCCGATCAAGCAGGGACTCCAACTTGCTAATAGTGTAGGGCTCGTGGATTCTGATTACAGAGGCGAATATTTGATGCAGCTCTATAATTTCACAAACCAGCCTGTAGAAATTGAGCAGGGAGCGAGACTTTGTCAGATCGAATTTTGTCCGCATTATCGAGGCACTCAACAATTTGGGATCTCTGAGATCCCAGCACTTCAGATCAAGATCGATCCTGAACTGTTTACGAACTTTGAAGAGGCTTATCCTTCAGTAAGAGGAGCCGGAGGATTCTGATCCACGGGAAGTAAATAATCCTCCTCCGTTGACAAATCTCTGAAAACTATCTGATTTTTTTCTTTTAAAAAAACAGAGGAAGAGCCACTCCTCTGCTCGATTCTTTTCTTTTTTGGTGATAATAACATAGCTTTTCGTAGTGTTCCTTTATTTTTCGTCGTGAAACCCATGAAAAATCTGATCAAAACCCCTGAACAAATTCAAAACATCACAGATTCCTGTGCATACCTTACTGAACTCCTCCATTACCTCAAAGACCATACGAAAGCGGGAATGAGCCTCCTTGAAGTCGAGGAACTCTCCCAGCAATGGTTGGATACAAGGAATCTCAAAGGAGCATTTAAGGGATATGAGGGATTTCCAGCAAATCTTTGCCTCTCTGTAAATGATTGCGTTGTCCATGGGATTCCTGACGAAACGGTATTAAAAAATGGTGATCTCTTGAAGATTGATACGGGGATTTTGTATAAAAAGGGTGTCTCTGATGCTTGTATTTCTATGATTATCGGTGATGCAGGGCTTAATCCGCTCGGAGCAGAACTTATCAAGGTAACTAAAGAAGCGCTTGATCGCGGGATAGCGAGCATTCAGGTTGGCGGTTTCGCTTACGACTATTCCAAAGCAGTATATAATCACGTTACGAATAATGGTTTTGAAATCATAAAAACACTGACAGGACATGGCGTGGGTAACGATGTTCACGAACTGCCTCATTTCTACAACTATCCGAACGCCAAGATGAAAAAAATTCAATGGATTCCGTGAATGGTGGTTGCTTTGGAACCGATCACTGCGGTAAAGTCCGATGATGTAGTCTATCATGAAAGTGATCGAAATCTTTACTGTAGAAAAGGTGATTTGGGAGCACAATGGGAATATACCGTGCTGATTACCGAGCATGGTCCTAAAATCCTCGCAGGAGTTGTTGAGGACTTGTATTAATCTAGGAAGAAACTCAGGATAAAATCACTATTGCTGGATAAAAAATGCTTTGGCGGCATTTTTTCGTGAAAGAAGGAAATCAGATCTTCTTTTCGGTTTCATCACGTAAGAAATAATAAAAATTAGCTTTTCTGCCTATGCGTGAATCAGACAAATCGTTGCTCTTATTATTTTTCTCCAGATAGAAGTGAGCAACAGATCCACAGAAGTCTTTGTGTATATTTTCTTTGCATATTTCTCAAGAGTAGGAGCTGAATCTCCTGCTTTTTTCAGATTTTTTCTGTTATTATCTTTGTGAGAATTTGCTTTTTTCGCCAGAATTGGCTATACTTGGAATAGCAATTTTTAATATCTAACAGAAGGAGAAATGGAAAGAATTTTCGTCTGCATTGCGTCGTATAGAGACAAACAGTTGCCGAAGACCGTGGCATCGTTATTGGAAAATGCTGAGCATCCTGAGTTGATTGATATTTGAATCTTGAACCAAGTAGATTTTGAGCAGGATCAGGCTTGTATGCTTGCTGCTCCAGAGGATTATCCTCAAGTGGAGGAACGCTGCATTGATCATACCTTATCAAGAGGATGTTGCTGGGCAAGAGCCTATGTTTACGAGCATCTTTTTAAAGATCAGAAGTTTGTTTTACAATTAGATAGCCATTGTAGGCTGCTAAAAAATTGGGACACTGAAGTCAAAGCAATGCGAAAAGGGCTTGAGGACTCTAAAGCCATTCTCTCACACTATGCTATGCCTTTTGGAGATGATGAGGTTTTGAAAGATGAATATTTTATCAGTTTTGAGTGCCCGCGATTCGATGAGGCTTATAAACTGCCAAGAATCCATCCTCATTCTTCTTGGGAGAGACCTGAGCAACCAAAGCAGATAGCGTTTGTGGGAGGTGGATTTATTTTTGGACTCGGAAAAGCCTTTAGAGAGGTTCCTTATGATCCCTTCCTCTACTTTAATGGAGAAGAGCAGACCTATGCCGCACGTTTATGGACGCATGGCTACAACATCTATGCTCCTCAAAAATCTATTGCTCGGCACTACTACGCAGACAATCCAGATCTCAAAAGAGTGAGACATCGAGAAGACAATAAGCGTTCAGATAATTTAACAGCGTTATCTATTGCTCGTCATAAGCATCTTTTGGGAATAGAAAACAGTGACAATGATGCTGTGATTAGATTCCTCAATCGTTATTGATTGGGAAAAGAGAGAAGTCTGAAAGATTACGAAAAGTTTAGCTGAGTTTGCTTTAAAGAGCAAACCATTGAGGAATTTGCAAAGAAAGGACAAGTCAATGAAGAATATGCCAGTGCTGTTGCATAAAAAAAAACTCTCTAGGAAATCTTTGATGAAAGAGCGCTATCAACGCTCTTTTTTTGGTTGTAAGGATTAGATCAAGATATTTTTTGTGGTAAAAAGTGATGGAATTTTATTCAGACTTTCATATAGTGGGGAAGGTAGTTTTTTTTGAAAGATGGCGTTTTGTCAAGAAATGTAAAAAAGGAAAGAAAAGTGAGTAAGCTACACAAAAATGAAGCTCTTACGAAATTTGAGTCAACAGGAAACCGAAAAAATTTTATTCTACGAAAGGTACTATGAAAATTATTTCTCGAAATGTTAATGGGATTAGAGCTGTACTTCAAAAGGGTTTTCTTGATTTTGTAAAGCAAGAGAATCCTGATGTACTTTGTCTGCAGGAAGTGAAAGCATTTGAGCATCAGTTACCAACAGAAATCAGATTTGCAATGCAGGACTATGACTATATTTGGCATCGTAGCGAAAGACCCGGCTATGCGGGGACAGCAATCTTTTATAAGAAGTCACTTCCAATCTTAGCAAAGCATAGTGCTTTTGAAAAACCTGGATGTTTTAATGATGATGGGAGAGTGACTGCGGTTGAATGGTCAGATTTTTTACTGTTGAATATTTACTTTCCTAATGGTGGAGATCGTGCTGATGGAACTGAAATGCTGAGTTATAAGCTGGATTTTTACAAGCAATATCAGGAGTTTGTGCAGCAGAAGGTAAAAGAGGGGAAGAAAGTCATCACCACTGGAGATTTCAATATCTGCCATCGTCCAATTGATATTGCAAGACCGAAGGAAAATGAAAACTCGATCTGATTTCTTCCTATTGAAAGAGCTGAAATGGATGCAATGGAGGCAAGCTGACTGATCGACGTTTTTAGGCTGCAGAATCCAGAACTAGGAGAGAAATATACGTGGCGGAGCTATAGAGCAGGAGCGAGACCAAGAAATGTAGGTTGGAGACTTGATTATTTCCGAGTCTCTGAGAATATGAGAGCTCAACTTGGGACTATGATGCATTATGATCAGGTAATGGGTTCTGATCACTGCCCTATTGTGCTCTTCATCCAATACGAATAAAACAAACTATACAAAAAACGGAAATCTGGATTCAGACTCCGTTTTTTTGAAATATACCGGACTATGATCCTCTGACTTGAGGACTCGAGCCGAAGAGCTGGATATCAGACCGTTTAATATCTCCTATCGCTTTTTTCTCGGGATTGAGACAAAGACGAGGAGACGGTCCTGGCTCAGAACCAGCTACTGAAGTTCCTCCGAATTGCTTCTTCAATCGCTCATATTCTTGCTTCCTGATATGGGGATCAACAAAGAAGAAGTAAATAGTTCCTGGCACCCTTCCCGTGACATGTGCCATCAGACACAAGAAAAGCGCAGCTTTGGCAATTTCACTGGGAACTGCATCAATCACATCATTATCATCACCTTCACGCCTCACGACTAGGTTAAGAGTGCCATTACTCACTGTAAAATGGTAACTAGCACCTGTTGACTCTGGATCGTTGAAGATCATAGAGCAATCCTCAGGCTTATTAATGATCAAATCAATTACCTTTCGAAGCGTTTTTTGACGCATTGAAGCTTCACTTTCGTCTGGGTTTTGCGCCGAATCAGAAGTGAGTGCTTTCTCTATCATGGAGAGCACCGCTTTACAAACGGCTTCAAAATCCACCTTTGGAGAGCTAAGCAAAGGAAAGTTGGAGATTTCCCTATTCTCTTGCTGGTTTAGCTTGATTTGCAACTTGTAGACCCACAAAGATATACACTCAGGATTTTTTCCTGGTTTCCCATCATTTACGATAGAACGCAGGAATGCTAAATACTCTTCTAGATATTCGTTCATTTTTTTGGAATTTAGTTTATTCTTTTGTAGATAGAATAGGCTATAGGATAGGGATTTTGCTCATCTCGTTCCATCACGTCATCGAAGATTTTATCGAAATTCTCTCGAATGGAATTGTGCAATTCCTCATCGAAATATACAGAATCGGTACTACGTTCAACCAGACCACAAATCCGGCTTATACATACTTTCGATACTGAAGGCATGAAAGCTTGATAAACCTCCATTCCTCCTACAACTACAATATCTTGCGATGTTTTCTCTGCATGGGATATTACCTCCTCAACACTTTTTGCTACAGGGACTGATTGTGGCAAGTGTATTCTTTTGCCAAGGCTAAAGGCCAGGCTCTTGCAATTCTCGAGCAATTTTCGGTGGGTCAATAATCTCCATACCTGCTCATCTGCAACAACCAATTTCCCGTTAACAGTCTCTTTAAAACTCTCTTCATCATTTCTCATGGGCCACGGAGTCAGGTACCCCTTTCCCATTCCTGCGATTACTCCATTTTTCGAAATGGAAACAATCATTATTATTTTTGTTCTCATTTTATGTTGTTTATTACGATTTTACTCTTTTATTATATAGACTTTTTTATAATTATTTTCTAATAAAAATCAAGGAGTTTCTTTTATTTATCATCTCAGATATTCCTGCTTCTCATCTCATCAAATCCTCTCTCAAAGGAATGAAAAACGGTCAATTTCCCCTCTTTGATCACCCAGAGAAGCTCTGAAGTTGCTTCCAAAAAATCTCTATCATGCGAAACGATCAAGGAAACCCCATTGAAATCTGCGAGCATCGTTTTGATCGCCTCTTTGGAAGTCAAATCTAGATGGTTGGTCGGCTCGTCCATGACTACAATATCTGGATGAGCAAGGAGCATTTTTGCTAGAGCGACCTTGGATCTCTCGCCTCATGAAAGGGTTCAGATTTTTTGGTCTGCTTTTTCTGGATCAATAAGCAAAGCCCCGAGAAATCTCATCGCTTCCTTAAATGAAACTCAAGGTCAAACCAGCTCTTCAGTAATCGTTGCTTGAAAGTCCAGATCATCAGCCACTTGGGAATAAAATCAAATCCTGACTTTTTCATGGATTCGCACATCGCCATACAAAGGTTTGAGCTGACCAAGCAGGGTTTTTAGCAAAGTCGTCTTTCCAACTCCGTTTTTTCCGATAATTCAGATTTTCATCGCTTTGGTAATTTCCAGTTCACGAGGCAGATTAACTAGCACGCTTTGCCCATATCCGACAGAAAGTTCAGAAAGTCTGATCAAGGTCTCTGGAAGTCTCCATTTGACCTGAAGATTTGGAGTATGTGCTGAAATTTCCTCTTCTGGAGCCTCAATACGATCCATCTTGTCTAGCATTTTGATTCTACTTTGCACCTGGGCGGCTTTACTAGATTTGTAGCGGAATCTCTCAATGAATTTCTCCTGCTGAGCAAGGTATTTCTGCTGTGCAGTATAGTTCTTGAGCTGGAGTTCAGCCCTTTTTTCTTTTTCTCTCAAGAAGTCTTCATAGCCACAATAGTAAAGATTCAGCTTTTTGTTTTTGATTTCTATCGTTTTGGTGAATGCAGAAGCCAGAAATTTTCTATCGTGAGAAATGCAGATCAGCGCCTTGTTTCGCATCTGACAGAACTGCTCAATAAAGAGAATCCCCTCAATATCCAGATGGTTGGTCGGCTCATCCAAAATCAGCAAATCCACATCTTGAAGCAGAAATTTTGCTATTTGCAACTTGGTTTGCTCGCCACCAGAAAGTTTTTTGATCGGAAAATTTAGTTGCTCTCTAGTAAATCAGAAGTATTTCAAAATCTCCTTTTGCAAATCATACTTTTGATAAGCCTCATGCATCAGCATCCGTTCAATATGCTCGCTTTGCTCCTCGATCAAGGCAATCGCATCGCTATTTCATCAGTCTAAAAGCTGTTTTATCTCCTCAAGTCTCTCTACTCTCCTCGTAATCTCCGGAAATATCGTGAGCATTTCTTCCAAGACGGTTCTCTCTCTTGATTCCCAAAATAAATCCTGAGAAAGAAATCAGATTTTTGTATCTTTTAGGAGTTCAATCTCGCCATGATCTGCGAGTTCAGGATTAAGCAGGATTTTGAGGAAAGTGGACTTCCCAGCTCCATTTTTTCCCACGAGAGCAATCTTGTCTTCAGGCTTGATCTTCAGTTCTGCATCTTCAAAGAGAATCGTAGAGCCGAGTTGTTTGCTCAAGCCCTTGATCGTGATGAGGTAGTCAGCGTTCTTTTTGTTCGTATAGTTGAAAAGATGTCTTGGAATGCTAGAAAACGAGACTTGCTTTTCACAGTCTTCTCCTGGATTTTCGGTTTCTCGCTGAATTCCAAATTCTTTACTGAGGTATTTTTTCAGACTTTTTTCTTTGGTGGATTCTTTGGTTTTATCGTTGAATTGAGCGAGGTAATCATAGCAAAGTTCAGCAATTTCTTCATAAGAAAAATCTGGATGAAGCGATTGGACAGATTCAATGATTTCGGAAAGTTGCATCATCTGAGGATGAAAATAAACGAGTTTAAATGTATGGAAAGCTCTCCTCTTTTTCAAGCTCAGTATGAAAGATGTCACATCTTCAGATTTTCTTCCGATTTCTTTTATAGAAGTTTTTTATTTTCTTTATGAATGAAGATGAACAAGTTACTCACGACGACACTTAGCCTCGGGGCAATCCTTTTATTAGCAGGATGTGGGACTCAAAATTCAGGTCCGACGATACAACTCGTAAATCCAGATAGTTGGAAAACAATGATTCCTGAAACTTGTCAAAGCTTTTTTGATGGATGTAACAACTGTACGAGAACTGCTGATGGAGAGGCTGCCTGCACGGAAATGTTCTGTGAAAGATATGAAATGCCAAAATGCCTTGATGATGAGCAAGGATGAGACCAGAAAGTCGGTATGGCAAATCCTGCCAGTGAATATTGTGTAGCTCAAGGAGGTGAAAGCAAGATCAAAAAGGATGAAAACTGAAGTGAATACGGAGTTTGCATGATTGATGGAAAGGAAGTTGATGAATGGGAGTACTATAGAGCTGGAATGGAAAAAGCAAGTGAGGAAAGTGCGAAAGCTTATATTGGACGCTCTGCCGAGGAAGTACAAAAAGAATTTGAAGAAAAAAATCAAATCGTTCGTATCGTTTTACAGGACGGACAAGCCATGGCTTTAACTGCAGACTACAGACCTGACCGCTTAAATATTGCCGTGGAGAAGGGAGTAATTACTAATGCTTACTTTGGATAAGCGTATTGGTAGTAAAAAAAAGCAGGGAAGAGAATCCCTGTTTTTTTGGTATGAAAGATATTGTAAGCTTGCTTGGATTACAATCAGTGAATGTTTCTTAGTCAAAAGACTAGCCACAAAAGGGAATCGAACCCTTAACCCCCGCTTTACGATAGCGGTGCTCTGCCAATTGAGCTATTGTGGCCGGATGGAAATGAGAGTAATGATTTGTGGAATAAAATCAAGACTGAAAAGTTGCAAAATAAAAAAATCTGAGTATGATTAGGCTGCTATTTTAGTTATTGACTACGTTCATGTGAATTGAAAATATTCAATCAACTCCTCGAGAAAAAAGCAGTATACCTCCTATTTTAAAAGCATTAAAGGCATATAGACCAACTATACTTAGTGCTCTTTCTTTGCCTCCTTTTAAGGATGGTGCAACATGAAAGAGGAATGTCTATGAAACTGAGATGTTTTCTACATTAGCTTATGAGTATGAGAAACTTCATCCAGTAGAGCAAAAGGATTTTAAAAAGGAGTTTAAGGCACTTTTGAAAATGTCACGAGAGCGTAGTGTTGCTCAAAAAGCTTGGAAAGACTTCTTAGAAGATTGGCAAGAGTTGTTGCCATTGACTTTTTGATATCAAAGTAATGACTATCAACAGCATTATCTCCCTAGAAAAAATTCCTTACAAGCTTTTTATGACACTTACTTGAATGCAGCGTAGAACTCATTTGGAATGATGTTCCCTAATAACGATGCATCTTACTAGCTTTAATCTGATTTTATTCTTTATTCTTAATAATACCACAAATGACCAATACAAAAGAACTCTTAGCATCTTTGAATGCGCACCAACTCGCTTATGTAAACCTCGATCTTCCAAACCCAAAGAATGGACAGTATATGCTCTGTGTCTTCCGCTTAGAGCCAGGAAAGAAATTGAATATGCTCCAAGCAGCCTGTGAAGTCGCTGCAGAATCTTCTACCGGAACGAATTTCCTCGTGGAAACTGAAACTGCATTTTCAAAGGAAATGAATGCTCTTGTGTATAAACTCGACGAGGAGAAAGAACTCGTATGGATTGCCTATCCGTGGAGGCTCTTTGATAGAGGGGGAAATGTTCAGAATATTCTGACCTATGTCGCAGGGAATGTCTTTGGAATGAAGGAAGTAAAGGCTTTGAAACTGCTTGATGTATGGTTTCCACCTGCAATGCTTGAGCAGTATGATGGACCAAGCTATACCCTAGCAGATATGAGGAATTACCTAGGAGTCTACAATAGACCGATCCTTGGAACAATTATTAAGCCGAAAATGGGACTTACCTCTGCTGAATATGCCGAAGTCTGCTATGATTTCTGGGTAGGTGGAGGAGATTTCGTGAAGAATGACGAACCGCAGGCGAATCAGGATTTTTGTCCTTATGATAAAATGGTGAAACATGTAAAAGAAGCCATGGACAAAGCAGTAAAAGAAACCTGACAAAAAAAGGTGCATTCCTTCAATGTTTCAGCGGCGGATTATGATACGATGCTTGAAAGATGTGAAATGATCGTGAATGCAGGATTTGAACCAGGCTCTTATGCTTTCTTGATTGATGGAACGACCGCAGGTTGGATGGCAGTGCAGACCTTGAGAAGAAAATATCCTGATGTCTTCATCCACTTCCATAGAGCTGGACACGGTGCGTTTACCAGACCAGAAAACCCTATTGGATACTCTGTTTTGGTGCTTTCAAAGTTCGCAAGACTCGCTGGAGCCTCTGGAATCCATACCGGAACCGCAGGAGTAGGGAAAATGGCAGGAGATAAAGCCGAAGATATTACTGCTGCAGAAGGAATCAGATATATGAAAAAGTCTGGTCATATCTTTGAGCAGTCTTGGGGAACGATTCCAGAAACGGATAAAGATTTCATTGAACTCGTGCAGAGAGATGAAGATAATGAAGAAGTTTTGAGAGATGATAGCTGGAGAGCAGTAAAAACTTGCTGCCCTATTATTTCTGGAGGACTCAATCCTACGCTTTTGAAGCCGTTTATTGACCTTATGGGAAATGTAGACTTCATCACGACCATGGGAGCTGGTTGTCATGCTCATCCAAAAGGAACGCAGGCAGGAGCTAAAGCTCTCGTTCAGGCTTGTGAGGCTTATCAAAAAGGCATTGATATTCATGAATATGCAAAGAATAAACCAGAACTCACAGAGGCGATTGCATTCTTTGAGAAGCCGGAAACGAAGGAGAAGATGAAAACTTTGAGAATCGCCGCTTAGAGCAAACGTCATCCAGTGCAATACTAGATTTTTCATCGCACCTCTTGATAAGAAGGTTGAGCAAAAAGCTTGCTCTCCTTTTTTGTAAAGGATGAATGAAGAGAAGGATCAGACTTTTTTCTTTTTGAAAAAGAGTAAAGAAAGCTGATTGCAGAGATGGAGCATGCAGGAAAGTTGAGTTTTCTCGCGTTGACTCCTAAAGAATCCTTCGAGGACTCAATATAACGAAAGCCTAGAATATCTACAAAGCTTCTCGACTTGCTTTTTCATATAAAATCGCTACCTTTGAGCAGTTCAAGGAAACTGTGGATGGGGAGAAGCAAACGTTGTAAGGAGACTGACTTCATCGAGATACAGTAAACCTCAAAAAAACTGATCAGACTTTATTTTCTTTTTTTGTATCATGGGACAGTATTCTACGCTTATCTACCAATCGGTCGACCACACTGCGAGGGCGCTCCTTGCACATTTTCAGGCACAATATGGAAAGCTGATCTTTATCCCACTTGGGATTTCCGCTAAGACACCCGAGAAAATCGCATTAGCCTCTAGTCTGAGCGTTGAAAGGCATTTGCTCCGTAGTTTTGAAGGCGAAGGAAAGCTGATGCTGGTGATTCAAAACGCTGAGATTCGTCAGGTATATAATGATTTTATGGATGGGCTCTATGGATCACTGAGCTTTGTCGGTAAGACTGCGATGAAAGCGTTAGGGATGAGGATGTATCGAGATGATCTTGATGCTCAGCTCAAGAATAATAAGCTCAATAAACCCAGCGTTTATGAGAAGCGAAAAGATAACCTTTTGATTATTTTGAGAGCATTATTTGGGGAGTTGCAGGCAAACCTTCAGCAGGCTGGAGTACAAGTGGTGTTTTGAAATGAAGAAGGGATACTTTCAAGTATGGATGAAGGATTCCAGGAACTCGCCATTTTTCAATAATACACTGTCAGGACTATTAAAAGAAAAAAATCCGCCCCTTTGATGAGGTTCAGCCTTGTTTTTTGAGAGACCGCCCTTGGATGAGGGAGGTTTTTTTGGTAGTAATAAAAAACTTGGTCTCACGTAGCTTATTGCTAGAACAGAGGAAGCATGAGAAAAGAGGAATTAGTCAGATTTTTTCTCTTTGAGATAGCCATTTTTGAAAGAAGTTCGGTCCATGGATTTTTTGCCCTCGGGCTTGAGGGAAAGGGTTTTGATAGCAGGATGAAGGCGATAGCTGGAGTCCCATAGCGGTTGGTCCTTGCCTGCGGCATAAAGATCTGCATCAAGAATCAGACTCTCAATGACTACCGTTTTTTCTTGGTGGGTAAACCAGATTTTGGGTCGGAGTGCGTAGGCACGATATTTAGCATAGATTCTGGCAAGAGAGTCACTCGCTGGTGCTATTTCACCGTCGTGTTTGGTAATCTTTTGACAGGTCGTGGACTGGCTTTCATCCTGAGGAGTTGCGTGAAGAGATCCCTTTGCATAGGATCGGAGGGTGTCATTGAGGAATTGTGGTCAGGTTTGCTGGATCTTTTCGATAAGGGTTTTGACGGTTCGGTCAAAAGGGAGCTTGAATGCAAGGCGGTCGACGATCGCTCCGGTATCCATTCCTGCGTCCATATGCATAATGGTGATTCCTGTCTGCGTTTGCTGATCAAGGAAAACGGACTGGAGCGGGCTTGCTCCACGATATTGAGGAAGGAGCGAACCATGCACGTTGATAGGAGCAAAAGTTGGGAGCTCAAGAAGACTCACAGGCATGAGTTTCCCATAAGCAATAACTACCAAGAAATCAGGTGATTTTGCTTGCAATCGATCGTAGAAGTTTTTGCCTTCAATAGAGGTTTCAAGGTTGATCTTGCGAGGAGTTTGGATTTGCTGCTCTTGGAAGCCCAGCTCAAGCGCGGTCTGTTTGATAACATTCGGGGTGAGTTTTAGTCCTCTGCCGACAGGCTTATCTTCTTGGGTTACAACGCCTACGACTTCGAATCTCGGGTCCTGGTGCAAAGCAGTTAAAAATGGAGTGCCGATTGGAGCACTGGAGAAAAAAACTACGCGGTAATATTCAGGATCATATATCTTTTGCATGGTTGGTGTGCAATGTAAAATTCTATCTGAGATTTTTGGTAAACCGTGCCAAAATCCGTATCTTTGCAAGCATTTCAAGCCGTATGCACTACAATGGGGTTCATGTCCGCAGATTCTTCCCTTTAGAAAGAAGGAAAGCAGACCCTTATCAGGCGAGAGGGTTGCCTGATAGCAACGGATGAGTCCAATAGTGATTCGAGCTAGAAAGCGGTCTAGTTTTTTCAGGGTAGAAATAAGCAGATGGGAAAGCATCTCAGAATCGCTTTATGTTAAAAAGAGCGGAGTGTAGTGGTACTTTTGGATGAGAGGCAGGATGTCTTGGTAGCTTTTCCCTGCGAGGATTCCTCAGGCAATAGTGGCATCTTGTGTAATATCTCATCAACATCGCTCTGGTTTTGAGAAGTTGTCTTTTTGGACTAGGTCTTGGAACTCAAAGTCTACCAGAACTAATCAGGATAATGCTCAGTGAAAAACATCAATTTCAGCAAACTCACCTGGGAGATATTCATAGCGGTATCTGGTTTTATGAACTTTCTTTCCCTCGAGCTGTGCAAAGAATTGATATTCCTCTGCAGTTAATACTAGGGTTTGTTCCTCTTGAATTGAAGCGTCCGTTGTGGACAGTGCCAGCTTTTTAGTGAGCTCAAAAGTTTCGCCGTCTTTTCTGAGTCTGATCCTTGGATGATCCAGGCTTGGGGGAAAATAGTAATCAAGTATTTCTTTTTTGGATTCAATGTTTTGATAGTTTCCGGTCTGAACTTCCTCGACAAAGGCAGGAGGGAATGACTGAAGCAGGAAGGTTAGTTCTAGTTCAAGCATTAGATTGTTAGCCTACGGAATAAAAAGTGTTTTTTAGCTTGGTATATCTGAGGATTATCCCCTTTGCGAACGGAGCTGAGCTGAAAGATACCCTAGTATGCAACCATAAGGGATAAAGAACAAATAATTTACCATACTATCTTCAAAGACGTGAAGGAAAAGCCCCATTACAAGAAAGGCGAGCATTCCCCTTTGCAAAGCACGAAAACATTGCCATTCCTCGGAAGTGTGGTCTCCTCTAAAGGAGTGATGGAGCTGTTTTTGCTGGCGTCCGAGCAAGAGCATCACTCCACATCGGAGCAGAAAACCAACCGTCCCGAGATCAAGCATTAATTGAAGATAATAATTCTCTGGAAGGAGATTACCACTGTGGTGTACAGCAGGTCAAGAGCTTCCAAGTCCGAAGCCAAGAGGATGGTTGGCAACCTCAAGCAGACCATTGAATTTGGCGGCTAAGTGTGCGATTGTCGAGTCTCGTTTGAGAACTGAGAAGCCAAGCAATCCGAGGCAACACACGATCCCTAGTCCTCGCATCAGCTTTTTATTTTTTTTGAGGCAAGAGAACTGAGTCAACATCAATACAATCACTGCTCCAACGAGTGCAGCTCTAGATAATGTTGCGCCCAAGGTAATAAACCAGAGCAGGAGGACAAGGGATTGCTGTCGGTGGGTTTTGAGTCGATTGAAACTTCGCTTTGGATTCCTGAGGCGGAAAAAGTGCAGGATCAAAGGGAGAAAGAGCACAAGGAAATAACCGTAATTATTTGGACCAGCGAAGAGGCCCTGTCGCCTGAGAATGCCGTTTTCTCCTGTGAGATAATAGATGGGAGGATTGGAGCCGAAATGGAAATTGTCAAGTTTACCGTAGCCTAGGGAGAAAAAGAAGTCAGGGAAGAGTAATTTGGCAAGCTGTCGGATTCGCCCTAGTAGGACTATGATTACGAGCGATCGTTTGATTCGTTTGGTAGCAGGAGTGAGGTTGGTTTTTTGCTGTGCTGAGAAGAAGCCGATCCCGCTCGCTGTAAGCAAGATTAGCATTCGCCAAAGGCCGTATTTGATGCCGATCAGAATGTCGCTAATACCTTTGTGGGAAAGAAAGAAGGAAGTCAGAATACCAAATATCAGCACTGCCCCAAAAGCACAGAAAACTTTTCGCCATTGCTGGAAGTATGCTTTCCGATGTCTGGTATTGAGCAAGAAAATAATGAGCGTTAGCAAAATCCATGCTCCATCTCTAAGCATTGCCAAGGCTGATTGGTCAATCCGTCCCATTCCATAGTCGAAAATAGTAACTAAGATATGATATCCCAGCTGAAAAATAAGGAAAACTCCCACTGCTCGGAGCATAATAACCTAGAGAAATAAAAAGTAAAAAAGCTGGTCTCAGTATGCAGAAGTAACTACAAATTGCAAGCAGAAAAATCTGAGAATAATTATCTGGATTTTTGGATGAAGAATCTTATAGTTTTATCCGTTTATCTATGCTATATTTATGAAAACACTTGCTATAGAAACCAGCTGTGATGATACCTCGCTTGCATTAGTAAACTATGAAGACTGAAGTTTTAAGGTAGAACAGCTCCTTGCTTATTCGCAGATCCAGGAACATCAAAAATTTTGAGGAGTAGTGCCTGAACTTGCTTCGAGACTCCATAGTGAGAAGATTATTGCTCTTTTGCAGACAATTTGATTTGACGAAATCCAGAAAGTTGATTTTATTTCGGTAACAACGCAGCCTGGTTTGCCCGGATCACTCGTAGTAGGGAAGACCGTTGCCTATGAATTGGGAGCGTTTTTGGGGAAAGAAGTAGTGCCGGTTCATCATATTGTGGGGCATATTTTTTCTATCTTACTTGAGAGGAAGCCCGAAGAGCTATCATTTCCTTTGGTTATCTTGACGGCATCAGGAGGGCACAATGATATCTATCTGATAGAAAAAAGAGAAGATGCTGAATCTAGACAGAATTTTGGCTCTTTTGAATTTTCAGGCTTCCGCATTACGAAAGTTGGTTGGACACTTGATGATGCTGCTGGAGAGTGTTTTGATAAGGTTTCAAGGATGCTCGGTGGACCTTATCCTGGTGGACTCCGAATCTCTCAGAAAGCTTCTCTCGGGAAACCTAATCCTCTCGTTCAATTTAAGAGAATCTTCCTCGCTCATGAGAAATTTGAATTTAGTTTTTCTGGGATGAAGAGTCAGGTTTCTTTTCTTTTGGAACATTTAGAGAGGGACTGAATCGCGTTGACGGACGAACTTGTCTGTGATATTGCTTATGAGTTTCAGGAAGCTGTCGTGGAAGTGCTTTCTAAGAAGCTTATTCAGGCAGGAGAACATTTTGGAGCGAAAGCATTGGCTTTGGCGGGAGGAGTATCGGCAAATACTAGGCTTTGCAACGCAATCCCAGAGCTTTTAAGTAAAAAACAGCTTACTTGTCCGTTTTTTATCCCTGTGAAAAAGGTTTATTCAACCGATAATGGAGCGATGATTGGACTTGCAGGACTGTTAACTAAGATCGAAGTGTAAAACAGATTTCTTTTGTAGATAACGAATGCAATTATTTCTTTAGCTCGGGTATTAGGGATGGATATTTTTTTTGATGAGGCGTGAAGAGGACCACTTTTCTGACCTCTTTATATTGGACTGGTGATTAGCAAGATTTCTCCTCAAGCTCTCGCCAAGCATCCCCTCTTCCAAGACAGCAAAAAATTGACTGCGAAAAAAAGGCAACTTGCGTTTTCTGAAATTCAAAGTTTAGCGGAGAGAAAAAAGATTTCCATCGCTATTGCGAGCGTTGAAGCGGGGATAATAGATTGCTATGGCGTTACTCAAGCGATTGGCATTGCGATCTGCAAGGGGCTTTATCAGCTGATTTGTCCTCTGATGAAAAGGACTTTGAAGCAAGAGTTTTTTCTTTCTGAACTGAAGGATTTGATACGTGAGTATGAGGATAGGCATCAAGAGAAAATAAGGCTAATTCTGGATGGGAAATCAGATTTTTGACTTTGAAAATGCTTGGAGATTGAGACGAAAACAATTGTCCATGGCGATGCTCAAGTCAAAGAGATTGCTATTGCATCTATTCTCGCTAAAGTTAGTAGAGATCAGTATTTAGAAGAGCTCTCTCATCGCTATCCTGCGTATTGATTAGAAAAACATAAGGGTTACTGAACAAAGGGACACTACTCCAAGATTCAGACATTTGGAACGACAGAGGAACATAGGAAACTTTTTCTCAAAAAGTTATTTCCGAAATGGACGATCCAAGCACTTGATTTTTCTACTTATAGTTTCAAAATATAAAAAACAACATTTTCTCACTTGACTTTTTTTGTTATTTCCGTATCTTTCGTGCAATTTTATTTCTCTGTGATAGTGAGAATGTCAACAACGTTCTGGTTTTGGATTATTATTGCTTTAATACTTCTGCTTTTGATCCGTATCTTTAGGAGATATATTGGAAGAATCATCCTGCTTCTTCTTTTTCTTTTGGTAGTTTTCTTTGTTTATAAGCGGTTAAATCCTCTCCAATGAGAACGTTTCTTACAGTGGATGAAAGGAGTTCCTGCACGTTCAGTAAACTTTGTCAATTTTTATCTCCTAAAAAATGATGTTGAGGCTCCTCTTCTTGAGGAAAGAGCGTCTACTAATGAACTGAAAGTATTGTCAGGGAATGTTTCTGATCATCTTAAAAATTCAGATAACTCAGCTAGTCAAGCTCCAATACTTAGGAAAGATAGCGAATCCTCTTCAGAGAGTGGATTATCTTCAGGTTCTAAAGCTGTAGAACTTGTTATTCTCAGTCAAGATACGACTCCTTCTGTGCAATCATGAGATAAAGAAAATCAGACTAACTCTCAAAATTCGGGTGTGAAGCAAGAGTTCGTCCTTGAAGCTCAAGAAACTTCTTATGAAGCGAAAGAATCCTCTCTCAATACTAAAGAGACTTTAGATGTAACTAAACAAGCTTCTCAAGGAGCTCAAGCACCAACTGTGAAAATCCCAGTCGAAACTCACGAAATTAAAGAACCTTCTCACAAGCAGAACACAGCTTCTGCTCCAATAAGCAGAACAACCAATAAAGTAGCATCCACCTCTACTTATCATTCAAAAGGGCTTACTGCTGCTGACTATGAGGAGGCTTGGGCAATTTTTTGAAGGAGATAGATGAAGGAATATGATTTAAACTCCTATTTGTTATCTTAACCATTAGTAGTTTTTTTGTGTTATAATTAATGAAGTTTATTTTATATTGACTTCTTTCTATATTTAACTATATTACAAAATGTGAATTAAAAAAAATGAATACTAATCAAAAAAAATAATCATTATGGCAACTAAAGAATGTTCTTGCTATCATTCGTATCCCGCTATTGAAGCTGTACTTCAAGCTGGAGCTGGGATGACCATCTCATTTCGGAATGGTAGAGGTTTATGCGAGGTCAGAATTGAGAAAGGAGAGAAAGTTTTGGTCTGTGTAGAGGGCTTTCGTGTTCAGGTTGCACTTCAACGGGCAAATCAAGAGCTTCTTGGTGGACCTTGGAATACTGGAGTCGCACCTAACCAAACGGTGGAAAGCTTCGTTGGTTTGGTAGACCTGAACGAAATCTCTAAGGATAGATTTGATTCTTATATCTTCCTTTGGAAGGTTACTCGGATTTTTTTTCATGAGGAAGTTGGATTCCTGTGTAGGGCAGGTGCAACCGAATCTTGTATATGGGGAAGTGGGAAAACCATGGCAGATGCTGTCATTGATTGCTGCCGAGCTACGAAGAATCCGACGAATAACTAGGATTAACAACAATTTAGAAAGCCCAGGATGGATCTATCCTGGGTTTCTTTACTAATTATTGCTTTTTTCAAGAGAGCAGGTTTTATTGGGGATTTAACTGCAGTTCTCTGTATTTTGATTCTGGCTTTTTTAGATGAAAATATCTCTCGGTTTTGCCCCCTCTTGAGGACCTATCACTCCTCTTTCCTCCAAAATATCCATAATTCTTGCAGCCCTTGCAAATCCAACATTGAGCTTTCTCTGGAGCAAGGTTGCAGAAGCTTTTCTGGTTTCCGCGATGACTTGGATGGCTTGATTGACGAGGTCTTCATCATCTCCAGAATCCCCTCCAGCAAAGGAACCTTTCGCAGTTTCCAGTCTGCTTTCTAATGCTGTAATAATTTCCTGATTATAAATATCGTCTTCACTTAGTCCTTGCATATATTTATTTCTCAGCTGAGCAACGACCTTTTCAATCTCTTCTGTTGCAATAAACGGAGCCTGGATTCTGATTGGGAATTTCGTGCTTGGATCCATATAGAGCATATCTCCCTTTCCAACGAGGTCTTCTGCTCCTTTACGACCAAGAATCGTCCTACTATCCACCTCAGAAACCACTCCAAAGGCAATTCTCGTCGGTATATTTGCTTTGATCAGACCGGTAATCACATTCACAGATGGTCTTTGTGTTGCGAGGATAAGATGGATTCCCACGGCTCTGGCTTTTGCAGCAAGACGGTTAATGCAGTTTTCAACTTCTTTTCTATTGGCAGAGCTAGAGAGCATCATATCCGCCATTTCATCAATGATAAAGACAATACGATACATTTTCTCTTGTCCTTCGGCACTCATCTTTTCGTTGTATTCATCCAGATTTCTTACTCTTTGGTCTTTGAGCGTCGTATATCTTTTTTCCATCTCCTCAACCGTTCGCTGAAGGAGTTTGATCGCTTTCCCACTATCATACACGATCGGAGCGAGCAAATACGGCAAACCTGAATACATTTCTAGTTCAACTTGCTTCGGATCGACCATAAGAAACTTGAGTTCAGCAGGAGTATTCTGAAACATCAGCGAAACGATAAAATCGTTGATTCCTACAGACTTTCCGCTTCCTGTCGCACCGGCAACAAGCAAATGTGGCATCTTTTCAAGCGGTCTCACCTGCACACTTCCATCAATCCCTTTCCCAAGAGAAAGATTGGTTGCATTCTTTTTCATCTCCGTAGAAAATTCGCTGGTATTGATCACATCGCCGAGCCTCACCATATTTGGTTTTGGATTTGGGATTTGGATTCCCACGAGATCGGTTCCTGGGATCGGAGCGATGATTCTGAGCGATTTAGATTTGAGGGAAAGCTTGATATCATTTGCCAGCCCTTCAATAGCAGAAATTCTGATCCCCTCATCAGGTTTAATCTTGATCTGCACGATAGACGGTCCAATATCAAATCCCTCAATCGCAATTGGAACCCCAAATTCCCCGAGCTTGGTTTGCAAAGCTTGTGCCTTTTCCACAAGGAAATTTTCATCAATCGTTTGTTCCTGTCCGAGTGTAGATTCTAGCAGTGATGTCCCAAAAGTCGGTTTATCTCACGAATAATGGATCACTGGTTTTGGCTTTTTTTCTTCAATCTTCTGCTCTACTTTGGCTTTAAACATTTCCTTAAGAAGAGAACCTCAACCAGAACTAGACTTGGCGATTTTTCCGGTGGCTTGAGAAACTTTTTCCATAATCGCTTCGGTAGAGATTTCGGGTCTCGTGATCGTAATCGTAGGTCTTTCCTGTTCTTTGGAGTCAGCTTTTTTTTCTTTTTTGATAGGAGTATCAGGAGTCTCGATATTGATTTTGGGAAGTTTTGGCAGGCGGACATTGAAAATATAAAGCAAATACCCAACCAACAGCACTAGAAGAACGATAACTATCGCCTTAATCGCGGTAATATAGCTTCCTGTCTCATTTTTAAGCCCTCTTTCTAAGAGCCGGATTAATGGCCATGAAAAATATCCTCAGTTTTCAAGCCACTTAATAGGGGTTGTAACATCTCGTAAGCGGATATTTAGCCAGGCAGAAGTCAGCATTAAGACTGAGAAAAAGTATTTTACTAGGAATTTGGTCAGATGTCCTTTAGCCAAAATGAGGAGCCCAATCACAATACACAATCAGAAAAAGAAAAGCAATCAGACTTCTCAAAAAGCGATTTTACTATATTTGGTAAAAAACTCGAATACTGGAGAGCCTTGTGCCATCGCTCTAGAAAAAAAATAGAGCACTCCCAAGGAAATCATTCCAAATCAAATATGATTTTTATTCACCGTAAAGGATCTTGCTGTTCTCCTCTGAGTTTTTCTTTTTGCCAAAATACTGGAGCTAAAAAAATAAAAAACTGAATCTATCGCACGTAAATGTAATGATTTTTGGCTTTCTTTCAAGCTAAACCAAAAGTTTTTGTAGCAAACACTTGTCTTTTTTCAAAAGTCTGTTATACTCTCAAAATGTGGCTACTAGGAGCAGCATTTCTCAATTTAAAAGAAGATAAACGATAGTCGCTATGCATCATTACAGCGATTTATATTTGCCATAAAAGATGATAAAAAAAGGAAGGCTTAAAGCAAGGATAAAAAGGTATTATTCTTTATCTCACTACAGCCTCCAAAAAAAACGAAAGGTTTCATTCAGGTTTTTAGTATCTTACCCCAAATTTTCAGCAACGAAGCCCCAATTGATCACATGGAAAAAATTCTCCAAATATGCTCCTCTATTATTTCTCGTATCAATATAATAGGCATGCTCTCGCACATCTACTCCAAGAAGGATTTTTACTGGCAAGTGAAGCGGATTTTGAGCGTTCGACATATTCAGAATTTCTAATTTTCCTTCTTGATTCAAAACTAGCCAAGTCCAACCAGAACCAAAATTTCCAACTGCTGAAGAAATAAACTGCTCTTTGAAACTAGCAAAAGATCCAAAATCCTGATCAATGAGTTCTGCCAATTTCCCACTAGGAACTTGATCATGTTTTGGACTCAAGCAATTCCAGAAAAAGTTATGATTCCAAAACTGGGCAGCATTATTATATAATGCTCAATTTGAGTTTTTTACAATATCTACGATATCCTTTCCAGCGAAATCGGTATTTTCAATCAATTGATTAAGTTTATCAGCATACGCCTGATGATGTTTTTCATAATGATAGCTCATCGTCTCTTCAGAGATGTAAGGAGCGAGTGCATCTTTTGGATAAGGAAGTGCTATTTTCTGAAAGGTCATTTACATTATGTATAAACAAATAAAAGATTCAGAAGTCTGAATAGGATTTTGGAGAGTTATGCTTTTTTCCCCTGGTTTGCTACAGCTTCCATCAGTTTTTTCACCTCTTCTGGATCTCCAAGGAAATAATCCTTCGTAAGCTTCATTTCATCGTCAAATTCAAATACATAAGGAACTGCTGTTGGGAGGTTGAGCTTTAGAATATCTTCGTCTGACATTCCTTTTAGCACCTTAATAATTCCTCTCAAACTATTTCCATGTGCAGCCACCAAAATCGTATCCTCTCTCTCTAGTGCTGGCAATATCGTTTCCTGCAAATAAGGCAATGCACGACCTACACAGTCTCTAAGGGATTCTGTTAGTGGCAAGTCTTGAGGTGCTACCCTTGCATAACGAGGATCCTGAAAAGGAGAACGTGGATCGTCTTTTTCAAGTGGAGTTGGCGGGATATCGTAGCTCCTTCTCCAAAGAAGTACCTGTTCGTCTCCGTATTTTTGTGCGGTTTCGGCTTTATTGAGCCCCTGGAGCATTCCATAATGCTTTTCATTGAGTCTCCGAGATTTTTCTACAGGGATCCAATCTAAATCCATCGTATCTAGCACGGTATTCAAGGTCTTGATAGCTCTTTTGAGGTAAGAGGTAAAAGCTCTCGAGAAGTGGAGATTTTCAGCCTTCAAAAGTGCACCTGCTTTTTTAGCTTCTTGGAGCCCTTTTTCGGTAAGATCAACATCCGTTCGTCCAGTAAATCTATTCTCTTGGTTTCGAGTACTTTCTCCATGACGGAGAAGAACTAGTTTTTTCATCAGTATTTTTCTAATAAAAGTAAAAGCAAAATCTCTTATAGTGAAAGCACTCAAAAGGTCAAGCTTTCCTCTGATTTTTATAGAACGCTTGCAATTATTTGGGAAAATACTAAACTTCCTCTGTTTATTTTGCTGTAAAAATGAAAAAAATCGGAATTGAACCACTGAAAAAGGAGTCAATTTCTTTTGGAAAGTTTGTTTTGCATACGGTTTTTCTCTCGCTTTTGGTGCTGAGTTTTACTGTTTTTTCTTTGTACTCAAAAATTACTGAACGGTTTTTTTCAAATCATAAGATTGATATCTTCACTGAACTACCTCAGGTTGCTTCTTATTTTTGGGTTGTTGACCCGGAGGTAGCTCGCCAGATTTTGCAACTTGATCTCATCATAAAAGATTACCTGTCTGGTGCTAACGTCCTGAAAACTAGAGAAGAGGAACTTACTGCACTTTGGAGCTATGCGAAAGAGAATAAAGACTACCTCACAAAGTTAGGATTTGGGAACTATAAGAGGATTTTGAAGATGCTTTCAGATGCACGACCACTGAGAGAAGAAATTTTCCAACTCCTCGGGAAAGATCAGCGTTTTAATTACCTTGTTCCTCTCCAAAACAGTAATGAATCTCGTCCTAATGGGGGATTCTTTGGGTCTTTTGCGTTTATTAGCCTTTCTGGAGGGCATATTGTCGATATGCAGATTATTGATTCTTATCTCCCTGATATGCTTGCTCCTAATACCCGTGTTCCTCTGCCTGAACGAACTCAGGGATTTCTGACAGAGAAAGCTGCGTGATTTATCGGAGGGAACAAATTCTGATTCACAGATAAAGACTGACGCAATCTGAAACAGCTCTATGAAAAGATCTTCCATACTGCTTTTGATCCGGAGAAAAGAGAGAAAATGTTTAATCAGGAGCAATGGAATCAGCTTTTTCAAAAGAATATCAAGGGAATTATCTTTCTCGACTCAGAATTAATTACCTATCTTCTGCCTGCCTTTCGCTCTAAAACGCGAGAATGGCAATTTGTCAATGCTAATGTCGATCTTATTAGAGGAGAGAATAGGAGTAATAAGAAGGAGTTTTATATCAAAGATCTTCAACGCTATCTGAAAGATAATGCTCTCACGCTTGCAAAGTCTACGATCAACAACATTCAAGAGCTCCTGCATAAGGGATTTATCAATATCTACCTCTCCAATGTCTCTGATGAAATGCGAACGTTCTTGAAAGACTATGATCTGACCAATGTGTATAATCCAGACTTTTTCTACTTTTTCCATATTAATAGCTCGTTTAATAAGTCTGATGGATTTGTCAAAAAACAGGTAGAGGTGCTAGGACCTCAAGGGAACGTTCTTCTCTCTACAGAAGAAAGAAAGCTGGACATTCATACACTTTCACCGGGAACCTATACCCTCCACATTAATTATACGCTTGATATTCCCAAGACCTATCAAGATGAGATCTTTGCACTAGAGAAGAGCTACTGAATCGAAATGACCGATAGAGAACGTCACATTCTTGCATTACAGCCAAAGAATCCTGATCCTACGATTCCTCTCAAATGGTGGGAAGCTAAAGCGATTTTCTATTTCCCAAAGAATGTAAAGATCCTTGGTATTCAAGGCGATGCTTTTAATGAAAAACAGTTTGAAGCAGATTTTGCCAAGGGGATTACCTATCAATCACGCATCTCTACCAACCAAACGACGAATACAATCAGTATCAAAATTCAGATTTTTTAACTTTTGGAACACATTATGCTAAAATTACTGCAAGGCTATTACCATTTTCTCTCGCTGGGGAAGTTTATGGAACAGGTTATTTTGACAAATGACCTTTCAAGTTTGGCTATGGATCTGCCCATCAGGACCGGGGCTTCTTCCTCCTTTGGACTTAAAGAATCCTTGATTAAGCAGTTGCTCAGCTCGCTTCACAAGCATCCGGACAAGAGGAATGTTTATGGCTATTTAATTGAAATTAGTGCGTTTAAGGGGATTTTCTCTGTGATGAGAGAAATTATAGAAAACGATGCCGTCCTGAGAAACTTTCTCAAAGATCAGCTCCAAGATCAGTATTTCCCTTTTGAACAGACGATTAGATTCTTGAGAAACGTGCTTAACCATTCTACATCCTCTGGTCTAGCACTCAAACTTGAGGATTATGAAGTGCAAAGAGACTTTATCTTGTCTCCCAAAATTCAGAAAACGAACCATCTCAATTGAGCAAAGATTCACTTAGATTTTGTCTATGCCGAGCATATTCCAGAACGGAAAGGAAGCAAGCACTACGGGATCCATTTGTGCTTAGACTTCGCTGCATTTAAACCATGAACGCCACTAGAACGCCTTGTGAAGTGGCACGACCTGTATCTTCTATCAGAATTGTGCTTTAATCTCTCGCAACTGGCTGATGCAAAACTCAAAGCCTTAAAGAACACAAGAAAAAAAACAGAATCCCCCTTGAAATCACGCACGAAAACAATACACTCCTCTCGTGGTTAATTTTTCAGTGGCTCAGATTTACGGATATCTTTCTCTTCTTCCTTCGCTGTAATGAATCTTGAAAAACTTTTTGGTAGTAAAACGAAAGTAGACATCCTCAAATATCTGATTTTCAGAAGGCAGGGCGTTTCCATGAGGGCATTAGAAACAGAAATCTGATGGACTTTCCCAGCAATCAAAAAACAAGTGGACAGTCTAAATGAAGCAGGAGTCCTGGGCGTTGAAAAAGAGCATACCTGATGGGTCATTACGATTTATCCACCGCTTTTGCCTCTTTTTAAGGAGCTTTTCTTTGAGGATCTCAAGCAAAACTTGGTGACGCTCTTTGAAAAGCAACAGCACCAGATCGTTCGCTATTTTTGGGGGGACAGGTTTGGGAAGCAGATCGGAATGGACCTCATCCTGATCTATGAACAGCTCGACAAACAGCAGATCGATCAACTCAAGGTGGAAATTAGTGAAATCTTTAGACAGTTTTGGATCGAAAGTGCCTCTATTGTCTTTATGAGCCAAGAAGAACGAGAGAAAAGATACAGACTCGCCGATAGATTCGTCCTTCAAGTGATGAGATTCTACAGCTCGGTCGTGAATTAGTAAACAAAAAGCTTTTTATTTTTTTTCTAACCTCAACGCTAACAATGAACTACGAACAACTCCTCGATGAAATCATGGTCAATACCCTTTCAGCGAAAGAACTGATAGGGACTCCGCAAAGAGATTTCCAATATGCAGTTTGTCCGTGGGATTTGGAGTTTCATACAATGATTTTTCTCTCAAGTATTCTGCTTACTTTCGATCAGAAGAAACCGATCATTGCCCTTATTCAAGTAGCGGAGTTGCCAGAACCGGTGATGCTCTATACCGGTGAAATTGGTCCGCACTTTGGAAGGCTACGAAACGTCGATAATCAGCCTTCTCAGCTCTTTGCTCAGCATGGCTTAGCGCAGACGGAAGAAAACTTTTACCCTTACCTTGATAAACTCTTCTGCTACCTTGGCGTGATCAATCCTAACCAAGAACATCTCGTTATTTTCATCAGGCAGGGAGTCAAGCAGACCGAACTGCTTGCACTCTTGAAAGAGCTTTTGGGGAAGGACTACTCACTTTTGGTCGTGAGCAACTGCTATGACGGTTTGCCTATGCAAGCGTGCAAAGCTCAATCTGAAACGCTCGTTGCACACCTGTTGGACAAGCAGATGAGTGAAGCAGAGCAAGAAGCATTCCCAGCATTCACGATTTTGAATCAGCTCTTGCCAAAGGAACTCGGAGAATCCGCAGTGGGACAACTTGTGAATACAGGAGAACTTGGATTTAGTCAGGATTCTTCTACTTCTTTTTGGTTTATGCTAGGATAGTGAGTATTTCTGCGTTTTATTTTGGAGGCAGTGATGGAAAAATCAAAGATTTTGCTCGTGCTAAGTGCCTGTTTCCTCGGGATTGGAGGCGTTTTGCTTGCGTTCTGGTGAGCGGAAGGTAAACTCAAAGCAGTGCTCTGTGCACCTGCTTCAAAAGCAGCCCAGGTTAGAGCCTTGACGAGAATGGTTTGGAGGGAGCAGCTCGAGAACTTCTCCCCTGAAGAGCACCGGACTAAGCAAGAGGAGCAGCAAAGCTTCACGCTCCCGATTCTTATGTTTCATTATATCCAGGATATTCCCGCAACGACGAAAGATCAGCTCGGCTACAGGCTGTCTTATGCCCCGCAGAAGCTTGATAAACTCCTTGACTTCTTTGAAGAGCACGCTATTACCCCGCTGACCTTCCGAGACCTCAAAGCTATCGTCGAGGGGAGACAAACCCCGCCCAAAAGAGCAGTAATTTTGAGCTTTGATGATGGGCATCTGGATCATTATACCAACGCTTTCCCCGTGCTGAGAAAGCATCACGCCAAGGCAGTCTTTTTTGTTATCGCGGGGAAACCTGATCAGGATCCGAGATTCGCAACTCGAGACCAGATCAGAGAGCTTGCAAGCGCAGGCTTTGAAATTGGCTCGCATTCGGTAAGCCATAGTAACCTCGCATCGCTGGGAAGCAAGCGTCTGAGGGAAGAAGTCTTTGAGTCTAAGGCGCGTATTGAAAAAGAAATCGGACTTCCCGTTCTTAGCTTCTGTTATCCAGCAGGAAGGTATGATGCCAGGGTCTTGAAACTCGTAAAAGAGGGCTATCTTTTTGCTCGCACGACCCAGCCTTGAACGTTTTTGCAGATGAAGAGACGTTATCAGCTCCCGACGGTCCGTGTTTCGCCAACGCTGCAACCAGAACAGCTCAAACGCTACCGAAACCTTGACTAGTTTTATCCTCTCCCTGCTCCGATGCACCTTTTATTCTGCTGTGCAACCCCTTGAGAGCTGAAAGCTGTGAAACAGCAGATCAAACAGCTCCAACTCAAACAAAAGCTTTCCCTCCATTACCTCTGCACCGGGGTTGGCAACTATGCGACCATCCTTTCCTTGACGAAGTTCCTGACCGAACAGCAAGATTCAGACTTTTTTCTGATCAATCTTGGAGTCTGCGGCTACCGATCCCCTGCAAACATCGCAAAAACACCTTTCCAAGTCGCTAGAATCAAAAACTTGCACACGGGCAAGGAGCTCCTCCCTCCTCTCCCCTTCCAATTCGCTCCCGTCGCTAGCGTCCGATCCTCAGAAACTCCCCTCTTGGAACCGCTCGGCAGTGAAGGCTTCGTCGAGATGGAGGCTCGAGGCGTGGAAACGGTAGCTGATGCGTTTAGACTGCCACGCTTGTTCCTGAAAGTGCCGTTTGATAGGGTGGGAGAGGAAACCCGTGCCTTTGATAAGGAGGCGGCGTGCAAGCTCCTCGCTGAAGCGATCAATTATCGTGCACTGCTCATCCAGCTCTTCGCACGAGAGCAAAGCTTGTAAAAGCGGTGAACGAAGCACTAGCAGAATGAAGCAAGAAAAAAGAAAAAATCTGAAGGAGAACAAGGGATTGCCTTCAGATTTTTGGTTTTTTTACTCTCAAGGAGAGTGATGAACTCTCGTTTTAGCTGCTAATTTCTCTGAGACTCGTGTTTTTCTCAAACCATAAGATGCAAACGGTCTCATCGTTATAAAACTCAAGCATATGTTCATGCCAGAGCATTTCTCTACATTTGGAGGTGGCGTTTTGCTCCTCGATAAATGCAAGTGTCGCATCAGCGAAGCCTCCGCTAAGCCTGAAAGGCAAATCTGAGAAGTGAGCGGCTCTTCCCATTACTATGACACAATTGTCTTTCCCAGCACGCCTAGGTCTCCCTGCATATCAACCCGCATACTCAGGGAAAAGACTACTATTAGACAACCATACTGGCTCTGAAAGGTAATTGATATACGGAAAGGTTTGATCTGAAAGTTCTTTATGAGGAACGTGGAACACTTTTTCTCCACTTCCAGGAGGGAAAATGATCGTTGAAGGCATATTTTCCTTTCTCTCATCCTTGAAATAGGATCAAAGACACCTTATTCCAAAGTCATACTTCCTAGTAACCGAGCTTGAAATCGCTGACAAGTCAAGTCTTGGGATTTGTAAGTAATCAGAGAGCACGTTTTGTAGAAGATCCTCCTTAGTAATCCTCTCGGGAGAAAAAAACTTCATGGTTATTGGGTCGAGCCTTCATTTTCACTCAAAATTGGGACTTCTCCAGAATACAAGCGTATACTTATAGCCACTTTCAATCTCCTTACCTCTTAGACTCCACCCCCCTCGCTTGTCCTCGTTCTGATCTATAAGTTCTCAGTCCTCCTCTGCTCCATTTTGATGAGCTGGAGGGACGTTTTCAGGGGAGGGAGAGGCTATTTCGCTTCAAGATTCCGTATTTTTGGGGAAGGACTGTCAATATTCTTCTAGCGAAGGGCCACATCCTGCCAACATGATCAATGCTAAGCAAAAGAGAAAAAATCTGACTTTCATTGTGCTTTCTTAGAAGATAAAGGATGCCCAAGTATCTCCTTTTTTGATGAAAGGAGGAGGGAAAGGCAAGTAAAAAGAAAAAATCTGACTCCTGCGAGTATATCGCTTTCCGTCCGAAAGTCAAAGCATCAGTTTTCTTTTTGTGTGATGAGGGGGTGCGTATTGAGGGAAAGGGATGGGCATGGAAGATTGCCATCGGAAACAAGCCTATGTTCGCTCTGTACAACGAAGCTTTCCCTGCCCCCATATCTCTCATCCTGAAGTCCATGTGAAGAAGTCATGATGAAGGTAACATTTTGTCTTGATATTTAAACTCCCTAGAGTATATTCTAGGGAGTTTTATTTTTCTATGAAAAACTCCTTATGAAACCATCTCCCCTCTTCTTGAGTATCGTTTGAGGGATACTCTTGACTGGATGTTCCCTTACTTCTCATCCAGAGCACTCCTCCCAGCAACCAAAAACCCCTATTTCGCAAGTAACTCTCACAGGACAACAAGACGCTTCGACAGGAACTCACAAAGAACACGATCAAACTTCAACGGGAACTACTAAAGAGCCACAGCAGGTGACTTCAAATCCTTCAACTACCTCTGAAACTCCATTTCGCCCGAAGTCTTACACCAAGCTTTCGTGGACAGGATATGAAGATATCAGGAAGATAGCACTAAAGAACTTCTGGATTTTTGAGTGAGATGATACCTATACCAATGATCCTTCTCGAAATATTGAGCTCCTCCCTCTCGAAATCGCGGAGAAGGATAAAGCGACCTATAAAGCTTATTACCTTCCTGAACCAAATCCGAGCCTTCCACCTGCGGATCCACTCGATCGACATGGAGAGGATAAGGATAACCTTTTTCTTGACTATGGCTTGATCGCTACCAAGAAAAGTAGCCTGCCTGGATATATTCCTCTCCAGTATGCAGGGACGCAGGCAATTATCGGACTGGTAGGATATGCTGATGGCTATCTTATTGTTACACCTTTCAAGTTTCCTGTTGATAAAAGCTCCTTCAAAGCCATTCAAGACAAAACCTTTCACGGGCGAGAAAATGATATTTATGCTCCGCTAACCTATGGTCATTGAGGGAAACGTCCTGGATTTTGGAATGGGGCACATTACTATGAAGACTATATCTGACATGATAAAGACTGGGTATATTTTCGAGACGACACCATTTCGGATTTAGGTATTAATAGAGTGAAAAAGGCAGCGAACTTTAAAGTTGAAGTATTTCCAGATGAGGTTTCTTAAGATGCTTTCCTTTACCGACTTACTCTATAGAGGAGCTTATCAGGACGACTTGAATATTTTCTCCTATTTACTGCATTGCATACTTGTATTTCTTTACTGCTTCTCAGTATAATCATGCTTTCAAAGTTAAAATCTCTTTATTTTTGAGGGAGCTTGCTTGCGATCTTGCTCTTGGGGCTTTCTTTTGTGGTAAAGAGGAGAGCAATAGTAGCAACCTTTAGTTTTCTTGGGGGGAAGGATGAGAACCTTTCTTTGGACTATATCCAATATTTTGCTCCAATGTTCTCTTTTGAGTATCGCTATTATGCGTTATTTTGTATCGGGACTAGTGGGCTGCTCTTTGTCGTCTATCAGATTTTTCTTTTGAAGCAAAAAGCTTTCACTCGGCGTAAATTTTTGTGGGCAACGGTGTGGTTTCTCGTAATTCTCGCAATTTGCTTTGTGATTTTATGGTTGAGTAAAATGTGGCTTGCTGAAATTTGGGAATAAGTGTAAGGCAAGAAGAGATGAACGGTTCTTCTTTTTTCTCTGGCAATATGCTTGTGGAGCATCCTCTAGTATGGAGGTGCTTTTTTTGATTGTTTTTGAGATGGAAGATAGCACTACTTTCTTTTCCCCTCGGTCATAAGAGCAATACAACGTCAAAAGGGGGGCTTTTTCTAGGTTTTGGGTGATTTTCCCTTGCAATTTGTGCTAGCTCCTCTATGGTAGCGAAAGAGTTCTCCTCTTCTACTCTCTAGAGGAGACGCTCCGACAAGAGTTTTGAGTATCTTACTGTCTGTCTCACTGCTTGAGCGTCTTTCCAGCACTGCTATGGTCAAAGTGAACCCAGAAAGACCTCCGCTGGCTTCGCTATGGGCAAAGTAGTCCCAGAAAGACCTCTGCTGGCTTCGCTATGGTCAAAGTGGTCCCAGAAAGACCTCCGCTGGCTTCGCTATCGTCAAAGTGGTCCCAGAAAGACCTCCGCTGGCTTCGCTATCGTCAAAGTAGTCCTAGAAAGACCTCTGCTGACTTCGCTATGGGCAAAGTAGTCCCAGAAAGACCTCTGCTGGCACTGCTTACGTGCAGAGTGCGAGGGGGAAGGATCGAGCATATTGCTCGGGGGGAGGAAGAAGGGAGAGAGGGAGGAAAGATGAGAAAACTCCTGTTGAACACAGACCAAACCAGAAGAGAGTATCAGCTTTTTTATCTTTTTATACTAACACAATGACAGACTCATTAGCAATTAGACCTTTTGGGATTACCAAGCTCAATCATGCTGCCTATGGTGCGTTTATGTTAGCTTTTGAAAAGCTGATCGCCAAAGGAACAGTGGCGAAATTAGGGCTGGCAGGGACGGATTTTGATGCGTTCAAGGCGAAGCTTCAACAATTTTTAGATCTGAATCAAGAACTCGGGCAACAACAAGAGACGAAAGAACTCTTGGAGCTGGACAAAGAAAGGGATCAGCTTTTGACCTATCTCTTTGCGAAGATTGCACTGGAAGCAAGCTCACCAGAATCAACACTGAAGCAAGCAGCAGAGGTTTTGCTCCCGCTCAGGAAGCAATATCTTGGATTACAGGTGAAAGCGAACCGTGAGGAGACGTTTTTGATTGAAGGGCTGTTGGTCGACAGCAAGAAGAGTGAGTTTGCGGCTGCATTCCAAGCAGTCGGGCTCAAGAAAACGCTGGAGCGTCTGGAAGAAGTCAACAAAAGCTATCAAGAAAAGACGATGGCTCGCTCTGAAGCGAATTTAGCACAGAAAACGGTTTCAGCGAAAGCACTGCGTATGGAGATGGACGCGTTTTATCAAGCAAGCACCAAGAAGATTGATGCGGTGCAGCTGATTGCGCCTGTAGCTGAGACGGAAGCGTTCATCAACTCCTTGAATCAGCTCATTGCCGAGACGCAAGAAGCGTGGAAGCTTAGGAAATCTCAGCAAGATAGATGGACTGAGGAAGGAGAACGAGAAGATGAAGCAAATGAGAAAGAAGATGCATCTGAAGAAGCAACAGCATAAGCAGAATTTTCAAAAAGAATGGAAAAAAGAGTGGCGGAATGCCACTCTTTTTTGGGAATGAGGAGAGAGGAAGTCTCTACTCTCTGATTCTAAACTCCATGGTCTGTTCGATAGATTGCTGATCTTCGAAATCCATCTTTACATTGATACGATAGGTTCCTTTCTTGGATCGGGTTTTGGTCATTTCGGTGCAGGTTCTACCTTCGCATTCATGGGCTGGGGTGCCGTCGCCGAAGTCTCGAGAGACTTTCTTCGGTAAGCCATTGAAATCAAGCGAAAAGCTGACAACATCTCAGATTTTGGCTTCTTGGGTAGGATGAGAATCTGGGTTGATCTCCAATTTGATAAGCTGCTTCTTGACGACAACCAGATTACTTGACTCTACGGTAACTGAACGTCACTTCTGAGTATAAACGGTGACCTTTGGTTTGAAGGTTCCGTTGTTTTTGGTGTAATCAAAGCGGTATCTGTGCTTTATCACACCGTTGGAAACCTTTTGCTGCTGCTGTCCATCACCAAAGTCCCACGTAAAATAGGTAATCTGATCGGCAGGATCATTCAGTCTGGACGATGAAGCATCAAGCGTGACCTCAAATGGTTCGAACCCAACGGAATCTCCAATCACTTGGAGCTTTCCTATCACATCATCTAAGCCGATCATCGTCGTAAGGTTCTCTTCATAGTCCAAGCCACGCACTTTATCTTGGATTTGGAGCTTGATTTTGTGTTGCTTGGCATCGGTAATATCAAAGAGGTATTCACCCTCGGTTGTAAAGACTACCGGTTTATCATCTAAGAAGACGTTTACTTTGGTATTGAAGGTTTTAGGTTCGATCTTGATGAGTTTGAGTTTGAGTTTGGTTGGGATCTCTGGGAGGGCGATGGTTTTCTTTTGCAAGATCGGATCCATATCCATTTTTTTGTAAGTCATATCATTGGGCGAAGAAGCATGCACTTCGTAGTTTACATTGAAAGAAGATTTTTCCAAGAGCTTGGCTTCGGTCTCACAGGAGCCTTTTTTATCATCGGTCGTAATGAAATTCATCCTCACGATATAGGTTCATTTCCCTGGGAAGGCATAAGGGAACTCCATCCCATTATCCGTATTATGAATCTCATCAATCACTTTTCAAGTTGCTTTGTCTATGAAGACGAAAGAATAATCACTGATAAATCTTTCTGTGCCGTCAAAGAAAGACGCTGAGATATTGTAATTATTTACCTTCTGCTGAGAAAGTTCGATCTTACAGACCGGGATTCCTGACTGTTCTACTCTGAGCGGGAAGGTATAGGTCAACTCTTCTGAGATTTCTGGGAAGCGAATCTTTGGATAGTAGACCTTTGCCTCATCATAGGTGAATCTAAAGGTTGCATCATCGATTTTATCTTCATTACCGTCGTTATTTCCATCCCAACGCACCTTATAGGAGCTCAAGTTGAAATCTCTAAAGACTCTGTCCGCCTTAAACTCCACCTCTGCTGGAAGTGGTCCTACAACGAGTTCGTTTTGTCCTCCACTGAGCTGTTTATTGAGTCCCTGGATCACGAGTTCAGAACCAACGACGATTTGTCTCAATGCATATGGCACGGTTTTCTTTTCTTTGTTTGGGGTCTCATAATGGATGATCAGTCTGGTAAGGTAATTTCCTTTTTTGGTATAGAAACAAGGTCTTTCAAATTCGGTTTTTTGGTTGAGGTTGATGACTGAGCCGTTTCCACAGTCTAGCTGAAGCCCGGTCACCTTTTGTGAACCAAGCTCGATATTTGTGAATTTTTGGAAGTTGGGTCGGATCACACGTGCCGTCATATTCATCGGAGCAATGAGCGGAAGTGGTCTCTCCTGCGTAATCTGAGTAAATTCATTTTTCTCATTAGGCACGCCTTTGAGGTAGAAGAGGACTACTTCATTTGGATTGGTAACGGCTGCGAGGTTGATCTCCTTGATCCTCGTCAGAACGTTGGTTCCCGCAACTAATGCAAGAATCAGAATAAAAAATCAGAGTGCGATTCCTGCGTAGTATCGTAATTTAGAAGAATTTTTCTTGGTAAACGCCTTGTAGACGTTGATAATCAGGATTACTATTCCTAATAGCAAGACTACGATCATAACCGTTAGGGTTAGACCGGAGAGCAAATCCTTTGCCACGGCTGCGGTAATTCCAAGACCGGCAAATCCTCAGAGCTGCTGAGGGTTCTTTAGGAGGTAAGAGAAAAGAAAAATGAGTCCCAGCAAAAAGAGAAACACAATCGAGCCACATCCCAAGAGAATCGTCTTGATCGAGATATTTTTTCTTGCGCCTTGCTTGAGGACCACGTTCTTATTAACCTGAACGTTGGAGTAACCCAGCGTTTTGAGCTTTTGTTGATTGAGCTGATAGTATTCACTCAGCTGTTTGAGCTGGGTCTGATATTTAATCATCTCCTCGGTTGGCATCTGGGAGTGCTGCTGAATATAGGCAACGACCTCATTATAGAGCTTCTGCAATTTCTCTTGCTCAGAGAGGATTTCCTTGATCTTTACATCATCATTGAGATGAGAGATTTCAGCCCCTGCGGCGGTATTCTGTTGCAGAAAACTTTCGTTATTTGCCATGTTCTTTTCTGAAAGGAGAAATAAATCAGCATTATTATAAAGTTTTTGGCAAATTTTGCAAATTTCTCGGGTTTTTTTGGCGAGTTCTTGAGGCTCTGGCGGTAGGAAATTTTTGCCTAGACTAGAAATCCAACAGGTTTGAAAAAAGGGCATCCTAGAGATGCCCACAACACGAGATCATTTCGAGCTGGATTAGTGTTTTTTCCATCTCTCCAAGATCAGCATCGCACTGATCGTATCAGTCGTGGCGTTCTTTTTGAAATCGGAGATGATTTCTCCTGATTGGACGCTCGTATAATCCTCCTCTACCGTCAGGATTTCAATTTTTTGGTTCTCTATGATGTAATTGAGCGACGCCATAAATTTAGAGATCTTTTCTTGGATGTCTTTTTGTTTGCTCGGCCGACCGAGAATGATTTTCCTAATATGATACCTCTGGATAATATCAGCAATATAATAATAAGTCATTTCATCATTCATAATATAGCCGATTGGGAAGATGATGTCTGAATTTTCTATTGGAGTATAAGCAAGTCCGATATATTTACTTCCTCGATCAATTCCAAGTGCGTGTGCGATTTTCATTGAAAAGGTATAAGATAAAACTAAAATACAGGTCAAGATAAAAGGTCTCTCCGCTTGGGTATCAAGACTTCTCTTAATCTAAGCATTAGGATACAAAAATCACCCACACTTTCAAGGATTTTTTAGTTAGATTATTAATTCCTATGAAGCAATCGCAATTACAGCATATTCTTGATATTTACGCTCTGGGAGAAGTGGAAAGTGTCGTCAAGAATAAGGTATGATTTATAAATGATGTTTATACTATCAACAATTCCTATATTCTCAAGGGCTATACTTCAGAAGACCCCTTCGCTCTAGAAAAGGAAGCATATTGCTATGTAGCGTTTAAAGAACATCTTCCTGTCCCCGAGCTCATAGCTGCGGATAGCTCTAAGAGGATGTATTCTCATAAATTTTTACTAGCGTCTAAGTTATCCGGTCAGAATCTCTACTCTGTTTGGCACCTTTTAAATAATCAAGAAAGAAGAGCTATTATTAAGCAGTTATGCTCATTTTTAAAAAAAATTAACCAACTTCCTTATTATGAATTTATGGAGAAGTTCCCTTCTCCTTATGATTGATCATGGGCAAATCAGCTCGTATCCAAGATAGAAGTATTGTTAAGCTTCTGTCAAAAAAAAGGCATCATTCCTGAAACTTTAGCTAAAAATATCCAAGCCTTTATTACATGCAATAAAGAAAGTTTAAACGAACAAAAAATCTGATTGGTTCACCGAGATATTCACTTTGATAATATCTTAGTTGAAAATATACAGATTACTGGAATGATAGATTTTGAAAAAATTATTGTTGGATCTCTTGATTTTGTTTTAAGTATGGTCAGAAGAATGTCATTATTCCCTACTAAATATATGTCAGAGGCAGATGAACCTCATGCCAAAAAAGAAGATTACGAAAATCTGCTACAACGATTTGAGGAGTACTATCCTGAGCTGTTTGACTTTAAGGATTTAGCTATTAGGTTAATGTTTTATGATCTTGAATATGATCTAAGGTTATTGAAGCATCATCCGCAGGAGCAATGAATCATTCTCAATTTACAGCATACTCTTGGAGCATTCTAGTTAACATTTGGTTCGATCATATAGTTTGGATGAAGCATTTCAACCTCTTGAGGAGCAAAATCTGCTCGAGGTAAATGGTAGGTTTCCCCTTTGAAATGCAAAGAGAGCCCCTGCTGCTCAAATGTATAGTCTAAAGAAGGAAGTGCTTCATCAAAATAGTCTCTTTCATACACTTGGTCTACGAAAAGCCCTTTATACTCTGAAGATAAAGCTGAGAGTTGGTCCTTTTTAACTGAGGAAATGGGCTTATTTTCCTCAAAGTCTCGCAGCCGAACATTGGATTTCTGTCCGATATAGATCGCTCCATAACGGGAGATCCAAGCTTTGCGATAGAAATGCTGATACAGCTCAATCTTTGAAAGAGAAAAGAAAGAAAGCTGACCCTTTTTGAGTGTTTTGAGGAGATTGAGTGCTAGGCAGCCTACCCTCAGGTTGGTAAACCCTCATGGTCCGTTGAGCACCAGTATTTCGGTCAGGTTTTTCTCTTTGTCAAGGTTTACTAAGACCTTTCCCAGCTCAACGTCAACTCCTGTTCTCTCCAGAAAAATAGGATGATCAAGTCCAAAAATTCTGATCTTATCCGAAGAGATGTCTATGCTTATCTTCATTTTGGAATTTATCTGTATAAAAAAAGGGTTTACTCCTTTCTCACCGAAACCGTTAGTAAACCCTTAAAGGAACTATCTTGTCCTGATAGTAAGACTAAACCATTCTTGGAATGCTGAATAGAATTCACTCCATCCTCTGAATTTTGCGGAGCTAGCGTTTCTTAGGACGTCTTGCATATTTCTATAGAAGTCTGAAGAAAGGTTTCTCCACTGCATGCTATTTCTTAGTCTTGGATAGTCTCTCTCTAGAGCTACGATAACGTCATTCCAAATGTTGGATACTGCTCTGATTTTGGAAAGCTCTTTGTCAGTGAAGTTGTAATAAGAGTTGGATCGAGAATTATTATTAGAAGAAGAGGAATATACACGAATCTCCTTTGTCCCATAGATTCCTGATCTATTATCATCCTCGGCTCTGATTCTAAATTCTCCTGTTGTCCTAAACTTTACAATATCTGAGAAGGTCTTTTCCCCGTTGTCATAGCGAGAGAAATTTACATACTGCTGATCTAGCTGGTAATCTGAGCTACTTGCTGTTCTCCATGATCCGTTTCTGTACTCCTCTACGAGAAGCTTTACCCTTCCTTCGTAATTTGCTACTTTGCTATTGTAGCGATCTTGTGCTCTTATTGTCAGATCAACCCATTCATCTGTGCGAGGAGAAGAAGGATAGATTTCTCTGATTGAAAGATTATCAACACTGTAATATGTAGAATTATTAGAAGAAGTTCCACCGTTAACATTGAAGGTTTGATAAGCGGTCGCTGTCCCGTATTCACGAAGCTGAGCAACAACTCTAAACTCCCCATTGGTTGAGAATTTTACGAAGTTATTTAATCTGACTTCTCCATTATCTGTAGATGAGAAGTAGTAGCTTGTCCTATCTAGCTGATAATCATAGCTAGAAGCATTTTCTCGTCTACCGTTTTTGTATTGTTCTACACGGAAGTCCACTCCTCCATAATAATTTACAGACCCATTTCTTCTTACTCTCAATGTCATATCTACTCGTTTATTTTGATCTGGATTTTTATCACTTATTGAGGTCCATTCTGGAGCATAATACTCAGAAGATGAAGAAGTCGTTGTATTAAACTCTCTGAAGACGGAATTTCCACCGTCGATTTCAGCATACAATCTGAATTGACCTTCTGATCTAAACTTTACTAAGCGATCAAATCTTTTTTCTCCATTTTCATAGGAAGAGAAAGAATAACTTGTTCTGTCCAATTCATAATCGTAGCTAGAGGCGTTTTCTCGTCTACCATTTTTGTATTGCTCTACACGGAATCTGATATTTTTGTCATAATTTGAGTAGGAATATCCGTTTCCTCTTACACGAACAGACACATCAACTCGTTGATCTCTAGCAGGATTACTAGGATTTGTTGAAGTAAACTCAACTTGGTAATTACTGTAAGAAGGAGCAGTATTGGATGAGGATGCGCCAAACGATCCGTTCCTAAAACTATAATTATCAACGAGCTCGTATCGATAATTTTGTATATCAGCCCTTAATTCAATCCTTCAGTCACTTCCTGCATACGAAAATTCTCCTTCACATCTTGGAAGAGCAACCTCATTCCCGTTTGGAAGCTTTAGTCTACAGGAGAGCTCAGAAAGAGATTTTAGATTTGAAGAACTTATTCTTGCCTGAAATCTGTCTGCATTTAATTTTTGGATATCAAAGCAGAACTTTTGCCCATCGGTAAACTGAATGTCCTGACAAGCTGCTGACGCAAAGGCTCATGGGATTGCAAGGAGTCCCAACATCCCGGTCAATAAAAGCGATCTTTTTTTCATAGCACTACGAGATAGAATGTAAAAATTGCTTGAATTCTATGTATATATATATATAGAGGAAAGGCAAGGTTTTTTTTGATTTTTTGAATGCAAAAAGAAAAAACTGAGCTTTTATTCGTGAATTTTACCTTATGAAGTGGTTAAAAATGTCGTTAGTGATCAGTCTTGTTTTTTTGATTGGTATTGGGACCTTTTATAGATTTTACTTTCTCCGCGATCCAATTCGTGCAGTTCCAAATGATGATACGATGTTTGTTAGCCCTGCTAATGGGAAAATTATCGCTATTATCAAGCAAGATGAACTAAAAGATCATAAAACAGAATTATATAAGGAAAATAAAAAAGTGATCAATGATTGGACGGAAGGTTTTTCAAGTGGGGCAACGTTGATTTCAATCATGATGACGCCGCTTGATGTGCACTACCAAAAAGCTCCCATGGGATCTAGAATGATTGATGAAGTTTATGTGGCTGGTCGCTTTTGAAATGCGATGAGAAGCAACTCTACGATGAATTCAACCTTTCAGAATGAATATCATAGTATGCTCTTTGAGACTCCGGAAGCGTATCAATTTCGTGTAATTCAGATTGCAGGGTTCGTAGCAAGGAGGATCGTTGACTTTTTAAATCCTGGACAAGAGGTTAAACAAGGAGAAAAAATCTGACTCATCAAACTGGGAAGTCAGGTAAGCATTGTTTTGGATAAAAACTTTGAAGTCCTCGCAAAGGTTGGAGATAAAGTAGTTGATGGAGAGACTATCCTTGCAAAGAAAAAATAAACAAGATAAAATTCAGATTTTCTTCTTTTTGTCAAATCTTTTCAGATAAAAAGAACTTGTATTTTTCGTTGATATCTGTATTCACGAAGATGCGTTTTGTCTGCTTTTTCAGGATCTCTAGCTTTAGAGAAAAGGAACTGGCAAACAAGCACCGTTTCCTTGATGTTTTTGTGCCTAGAGATTTTTTATCTAGCTTGATGTCAAAATATCAAATTAGAAGCGGAGACTGAGTTACTCAGTATTGTTTTTTTATCCCTCTTATGGAATGGTATCTATGGAAAAATGTAAAAATGAAAATTGTCATGGTATGATGGCAGGAACAGCAGTTGCTTTGAGTGCAATTACTCTTATTTGAGGTGCAGCACTAGGAACTTGCATGTACCAAAAAGTAAATTCTATTTACAAAGCAGCAATTGAGATGCAATATGGATCTCAAGCAAACTTTGAAGCGTATTTTGAGGCTAGAATGACGCCAGAAGTGAGACAACAAATTGATAAAGGTATTAGCGAAACTATTACACAGTTAAAATCTCAAGGAGGAGCTACTGATACTGCAAATACTACAAAAGAAGAAAATACTGCAGCACAAGATAGTTCAGCACAAGGATCTCAATCTGGAAAACTGACTCAAGAGGAAATTGCAGGGTTTATCGGTTCAACTCCTAACTTTGCAGATAAACAGGGGAATATTTTAGTTCTTTCTTATGTTGATTTTCTCTGTAGTTATTGTAAAAAACTCCACGAAGCTGAAACTTTAGAACAGCTTAGTCAGGAGAATGAAGACGTTAGCGTTGTTCTCAAGTCTATTCCTCTCTTCTGAGAGCAATCAGAAGCTGGTGCTCATGGAGCGTATTGTGCTGCACAGTTTGGAGGAGCAGAGAAGTACTATGCATACGTGGAGAAAGCGTTTTCTAAGCAAGCAACAAGTGAAAGCGATGTAATTGCTCTTGCAAAGGAAATAGGACTTGATGAAGCTCAGTTTAGTGGATGTCTTAGCAGTGCTGATACTAAGAGTGCTGTGAGTGAAATCTTTCCTGCAGCCTCAAGAAAATTCGAAATTACTGGAACTCCTGCAAGTGTAATTATCAATACAAAAACAGGAAAATACGAAGTTATTCCAGGAGCTTATCCAAAGTCATACTTTGAAGAAGTAATCAAAAAAGTAAGAGGATAATTCCTTACAAACATATCAATCTATTAAGCTACTATGGAGATATCTATGGTAGCTTTTTTGAAAAAGGGCGAGAAAAAGGAGATAAAATAAGAGAAAAAGTCAGACTTTTTTTGACTTTTTTATTTTTTAGGATACAATAGAGTAGAAATGCTTTTTATATCATCTTTGCAAGTTAGATGACGGAAAATCAGACACCAACCACCGTTCAGCTTGATAGTATTCCTTCTTGAGAACCTCCTAAAATGGGCTTTTTTGAGAAGACGGTAAAGAATTTTGTAGGGTTTATAGCGAAAGTTACTGGACAGGCTGACCCAAAACAGCAAACGGCAACGCCTCCTGCTAATGGGACTTCAAGCTCCTCTCCTGCTACAGCCACTACTCCAAGCGCTGACCAGAATAACGGAGGAAGTGATATACCTAAGAAGGAAGCAAGCTTTTTCGATAAACTTGTTGCCTCGACCCAAAACTTGTTAAACAAAACTGAACAATTCGCCACCAAGGCCACAGAGAAAACAAAACAATTGACTCAATCTATCAGAGAGGCTCCTTCAAAGGTAGTAGACAAAACAAATACCGTCATCCAGAAAGGAGTGAACTTTGGGGAAAAGGTAAAAAATCAGGCAGAACAGACGATTGATAAGGGAGTTGATTTTGGGAAAAACCTACAAAGCACGATGAAAGAGTGAGCTCAACAATTTAAAACAAACCCCATTCAAGCGGGAGGAAATGTTATTAGTGAGGCGGCTCATTCCACGGCAGAGTTTGGGAAAGGGTTGGGATGACAAGCAAAGGAAATAGGAAGCAATGCCGTTGCGAAAATCAAAGACACCGGCTCTACTATAAAAGAACATATTCATAACGCAGGGCAACAAGTCAACATTGATGCGAGCGGGGGGATTAAAAGCGTGCCTACCAATATTGTAGGGGGGATTAAACAGGCTGGAGCTAAAGTAGTAGGGAAAACTAAAGAATTCTGACAGCATGCCATCGATACGACCAAAGATATTATTAAAAATCCTGTTAAACATATTGATAACCTCGCGAGTGATGGGATGTCGTCACAGGAAGCCGTAGTTACAGAGACAGAGAACACGACCTCTCTCGACCATTTGGAAGCCTGACTTCCAACTGCTTCGACCCCCGAAACAGCAGAAGCAACAGCGCCAACTCCTCAGGAAACCACCCCTGCTCCTCAAGAAGTAATTGCTACCACTCAAGAAACTACCACTGCTCCTGAAGTAGCCACACCTACTCCTCAGGAAACTACCACTGCTCCTCAAGAAGCAATTGCTACCACTCAGGAAACCACCCCTGCTCCTCAAGAAGCAATTGCTACCACTCAGGAAATTAGCACTGCTCCTGAAGAAGTAACATCTACTCCTCAGGAAACTGTAGCTGCTCCTGAAGTAGCCACACCTACTCCTCAGGAAACTCCTCCTGCTCCTCAAGAAGTAACACCTACTCCTGAGGAAACTAGCACTGCTCCTGAAGTAGCTCATGAAGAAACAGTGACCGTCTCTGAGGTTCAAGCGGAGGAATCCACAGCTCCAGAGCACGCAACGCCCCCTTCTCCAACGATGGAAGACGCAAACCAGCCACTAAGCTGATCCTCTTCCGATAGTCCAGCTGACGCGCAAAACCTTCGTCCTGAGGCTCCACAGGGAGCTTTGGAGGGGGCTAGTGTAGACCAGGGGTCATCACCTGTAGCACCAGACCAACCAACAGATGAAAAATCAGATTTTTCTTCTCCTGAAGGAACCCCTCAACCCGTTCAACAATAAGTGTTTAAGGTTGAGCGTCTAAGGCGTTGTATGAAGGCAGGCACTGCGCTCTCTTGGAGGGGGCGGAGGCAAGAAAAAATTGAGAACAACGAAAACCAGCACACCAAAAAACCCTACGCCACCATCAGATTTTTCTGGTGATTCTGAGGTGCAAGGTCTTGGTAAGCTGGTTTTTTTGGTTGTTTGAGAATGCGGGAAAGCTTGCAAGGGGTATTTTTTGGGGTTTGCAAGGCTTGCAAGGGATCGGAATGGTGCATTTTTGGGGTTTGCAAGACGTGCAAGTGGTCGGAATGGTGCATTTTTGGGGTTTGCACGAGCTGCAAGGGGTCGGAATGGTGCATTTTTGGGGTTTGCACGAGCTGCAAGGGGTCAGAATGGATCATTTTTGGGGTTTGCACGAGCTGCAAGTGAAGAAAAAGGGGGCTTTTTTGTCGTTTCACGGTGTGCAAACCTCCTGCGAGGGTCCTTTTTGGTCGTTTCACGGGCTGCAAACTCCCTGCGAGGACCCTTTTTGATCGTTTCACGGGCTGCAAACTCCCTGCGAGAATCCTTTTTCACTCTTTGCATGGGCTGCAAACTCCCAGCGAGGGTCCTTTTTCATCGTTTCACGGTGTGCAAACTCCTAGCGAGGGGGCTTTTTTCAAAGGGGGCGGAAGTTGCAACTCGGAAAGTAGGAGAAAGTCAGACTTTTCTCTCTTTTTACCTCTTCAAGAAAGGGTGGAAATCTTGGTGCTTTTCTTTAGAATGCGGGTGTTTTACCTGCGTTGCTTATGAAGATGTGAATGTTGCTACTGGGAATGTTTGCGATGTATAGCTTTCTTTTTCCTGTGCTCCCTACTGAACCTGAGACTCCTCATATATGGGAGAGTGTTGACGGACAGAAATTTACCCGGGAGGGCTACCCTGCCATTCCTGATAACACTGAAGTCCTTTGGTGGGACTGGGATGTTGACTATCCTCCTGGGACGAGGAGAGCTTTTTTTGTGAAGGGACAACTGAAGAGAGAAGTATTCTCGTGTGAGGAGCTGAGTGAATGTGAGTTTCCTCGGGGGAAGCCTCGTATGGAGTTGTGGTCGATCATTTCCCAAGGAAAAGAACTCCACTCTTTTGGAGATTCTATTTCCTTAGGTATGACTTCTTGATGAAACATTTACTCTCATCTTCCTGTAGAGAGAAGGTATCCCATCGCTAACTTTTGAGGTTATGATCTTCGTAAGATTGCTAATAATGGGAAGGAATATCTGTTTTTTGTTTCTTACAACGTGCTACCGGATATGGAATTTAGTAGTATTGATTCTGCGGTAGAGGATCGACGGATCATGGCTGGGCAGTAAGCTCTGGGAGAGGTTATCTCAGAAGAAGCTATTCTGATATTGTTTTTTATAATTGAGCCTCTAGATAGGGGCTTTTTTGTCGTTTGCGAGGGGGCTTCAAGGGGGGAAGGATCAGATTTTTTACTCTTGAGCAGAAAGGTCTCGGTTAATTTTGTCCTTTCCGATGCTTTCGAGCATTTCGTTACAGGTGCTAAACGGTTTGCTTCAGAAAAAGCCTCTGTCTGCCGAGAATGGAGAAGGATGCGGAGACTTGATGATAAAGTGTTTGTCTCTATCGATCAGGGTTTCTTTTTGTTGGGCAAAGGCTCCTCGCAAGATGAAAACGAGGCCGGTCTTTTGGTCTGAAAGTTTTTTTATCACCTCGTCGGTAAAGTGGTGTCGTCCGATGTCTTTGTGCGAATTAGGGGCGTCTTTGCGGACGGTCAGTCCTGCGTTTAGGAGAAGGACGCCCTGCTTTGCTCGTTGTTCGAGGTTGCCGCTCGCAGGAGGATCGATATTTAAGTCAGACTTTAATTCTTTGAAGATATTTCTCAAAGAAGGAGGAATTCTGACCCCTTGAGGAACGGAAAAACTTAGTCCATGCGCCTCCCCTTCCCCATGGTAAGGATCTTGCCCAAGGATAACGACCTTTACCTCATCAAAAGGAGTGAGATTGAAAGCATTAAAAATGTCTTTCCCCTGTGGATAAACGATCTGACCTCTCTCTATTTCGGACTTTAGGAAGGTTTTGATCTCTGCAAAATAGGGTTTTTGAAATTCATCCTGCAGAACAGCTTTCCGTGAGGATTCTATCGCGATAGGTTTTTGCTCTGGCATCAGCAATGGACATCTAGCTATATAAAAACACAATGAGCTTTAGCAACAATCAGCAACGAAGCTTAAGCAGCTCGGTATATTGTTCCAATAATATATAATCCAAGAATCAACATAAGGAGTCCAACGATGAGGTGGATCAGCTTTGTATGTTTATTCTTTCGCTTTCAGAGGGTTTCTGCGGAAGTTCGTCCGCCACCGACCATCACGGTGATGACGATCATTGGAAGGATGAAAATCAGATTATAAAGCAGAAGGTATGCGATTCCCAAAGAATTTAAACCGGAGCTTTCTGCACCGAGCAGGAGAAGAACTGTTACGTAAGGTCCTGACGAACACGGTAAAAGGAATAGACTCACGATAATCCCGATAATAAACGCTCATCCTGGAGAAACTACCGCTTGGATCAGTTTCATCATTTTAGGTCTCCATGCGAGTGGAACCTCCATAACAAAGCCTTTTCCATACCAGAAATAATCTTTTAAATTTGCTAGCCCAATCAAAATTCCGAGAAGCCCGACTCCTCGCTTAAAGATTGTCGTATATTGCATGGTTGTCGCGTAAAGAAGCTTTAGAAAGCCCAATCCTAGAAGGAAGTAGGAAAGGAAAATTGCCAAGCAGAAAAGGATACCTGCGAAGATTGTTTTCTTTTTACTTTTGGATTTGGAGAGGATAGTCGCTAGTAAAAGAAGCATTACAGCAAAGGCACAGGGATTAATACTATCAGAAAGAGCTCCTGAAAGCAGAATTGGGATAAACTTTTTGGGCTGTGAAGCGATATTTTCCTCCGTTCACATCTTAGTAGCTCAAGAGGACAGGAGTCTGGTTTCTTTACTCGTATTTCAAGTTTGTGTGGTTCAAGTCGTTAGAGCTAGCTGCCCTGAGGTTTCTGATTCTTCTGTGTTGTTGTCCTCCACCTTTCCACCGGTCAAGAGTGTGTTTTGTGGTGAAAGTTCGGTTGGATTTGCGGAGATGCTCGATCAGGTTTCAGCTTTTTGATCTGCTCACTCGTTCGCAAGAGCAGAAAAGTATTCAATGATTGGGGTGCTTCCCATGAGGTACTCTTTCTGTTCTCCGTTTATCATTACAAGAAAAGGGGTTCCGACGTCATTTAGGGATAAGCCGATTGAAGGCAGGATTTCTACAAGCTTTTGACGCCCTGTAGCGTCGAACCATATTTCGTACTTTTGGAGCTGAACCTTCTCATCGATATCAGTTTTTTCAAAATATTTTTCTACGGTCTGACAATGCCCACATCCTTGACCGTAAAAGTAAAGATAAGTTGCATTTTTTTCATTGGCACTAGAAAAGGATGTTAATAATGAAAGTGCCATTACTACAAGTGAAAAGAAAGCTTTTTTAACCATAGGAGGAGATTAATTCTGAGATAAAAACTGATCGGTTTGTAAGGATAGGAAAAACTGCAGGTTTTTCAAGGGCAATTTGCTTGAGTATTCGTTTCCTCTTGCAAGAGACGGGCATTTCTTTATACTAGTGTCAGTATTTTTAGTTTTTGAAGGCAGATGAAACCGCTTTTGGAGAAATTTTTTACAGCGCCAGCTCAACAAGCAGTAGAATCGATACTTTCTGTTTATCAGCAGCAGAAATTTGCTGTTGTGAATTTTGTGTATTTTGCCAACATTGTTGCACAAAAGCTTTTTAAGGATAATAAGTATAAAACAGAAAAAGAGAGAGAATACAAGAAAATCCTTTTAAAATCTGATTTTTTACTACCAGATGGTATTGCACTTCAGATTTTTTACTTTTTTGCGAGACTTTTTAGGCGTATTCATACCGATCGCTACCGATTACCCAACTTAAATGGGACAGATTTTGTGCCATATTTTCTTTCTGAGGCGAAAAAGAAGTATGGAAGCCAGAGGTTATGTCTGCTCCTCTATTGAACAAAAGCGGAATATCTCAAGATCGTTGAAGAGAAGTTAAAATTCCAAGGATATAATGTTATTTACAGTCAGGATGGTTACTCTGATTTTGACTGGGTTCGTGCACAGACTGCACTAAAGCAATACCAAGATACTACCAATATTCTTCTTGTAGCAAGGTCGACTCCTCAGATTCCTTTACAAGAGCTTTGGACAAGTAGAAATTACCAGAAAATCCAGCAAAATGAACTCCTTGTTTTTAATACTGGAGGATTATTTGACTTTATCTCCGGGAAAGGTCAAAAGAGAGCACCAAAACTTCTGAGAACCTTGAAAATGGAATGGCTCTATAGAGTGATTGTTGATCCAAAAAGAAACTTTAGGAAAGTGCTCAACTCATTTGCAATTCTTCCCTACTTGTTCAGCTATGTTGTATTAGGGAAAGATAGCCATAAAAACTCTCAGTAAAAGATCAAGACTACCCTATCAATGCGAAAATTCCCAATGTTGCGATAGTAAGTATTCCTCTAAATACTCCCTCAAAGAAGCTAATAAAGTGAATATGTTTTAGGAGGCGGGTGAAATTCTGCTCTTTTTCTCCAGGGATTACCCTTTCTCCAAGCAGACTTTCGACTTTGTGCATATTTTCGATGATAAGGGAAGAACTCTGAATATAATTATAATTCCCCTTGGTAAAAATACTTTTCATCAATGCAACATTTGGGCTTCCTCCCAAATTTTTAAGATTTAATTGCTTCAGATGCTGAGTCCTCGCTAAAAGATAAATCATTTCGTCGACAGCTGCTACAACCTCCTTTTGAAGTCTGATTTTTTTCTTTTTTAGCAACCTTTGCAAAGGGAGGATTAACACAAAAATGATAAGATACGAACAAATTAATCGAAAGACAATCGTCGCATATGGCTCTGAAAGTCAAGTGATTTGCTTGAAACTCTGAAAGATAAATAAGCTGACGTCTAAGATCTTTGTTCAAATTTGGTGTAGAATTTCCATTGCTGAGCGAGTAAGGTTAAAAAATCAGATGTGCTGGCTCAAAGACAACTATAATTTTTCCATCTTTTTCTGCAAGAGGAAGTGAGTTTCTTCGGAAGATAGGGATTTTTTGGTTGATTGCTCGCTTTTTGAGGAGCTTTCAGTGGAAATAGTCCCCCGGTCTTGAAAATCTCAACGTTGCATTTATCAGTTTTGGTGAGATATCTAATTGAAATTTTCCGAAAGATTGCACTCCTCCTTCAGTAATTTTTTTTTCTAAATGCAACTCTTTTTCCCAAAACCTTGGCTTCGCCTTGATGATAAATACTTGATCGTGAGAAAGAAAGATCGTTCGTCCACCTAGTTCCTGGATTCCAGTTCCATTAGCGATCCAATTTTCTAATTCTTGAAGTTCTCCCTTTCTTACCGTGATATCTAATGCCTGCAATAAGCTTACCAATACCAAAGTATCTTTTCTGCTATTTTGTGGGATTATTCGTTGAAATGCCTCTTCTGCATTTCGATAGGGATTCTTTTTGATCGGGATAAGCCGTTTTAGAGGATCAGATTTATCTTGCTTTTCTAACTCATGATAAATTGTTTTTCGACTTTCAAGAAAGGAGCTTCCTTGAGCTGTTTCAAAGCTCAAGTCTTCAAAAATGGGAAGAATCTTGTTTCTGATATGGTTTCTGAGGCTAACCTCTGGGTTTGTATTGGTCTCATCCTGAAAAAAGGGGATTTGCAGACGAAGACAGTTTTCTTCGATTGCTTTTTTGGTAAATCATAACAAAGGTCTCATAAGCTTTATGCAAGAAGGAAGGAGGGGATGGGCGTCCTGAATATGCATATTGAGCAGCCCTTTTACCCCACAGCCACGAATGAGGTTCATCATAGAGGTTTCGATCCTATCATTAAGATGATGTCATAAAACAAGCGCTGAAATCTGATTTTCTTGGCAGAACTTTTGGAAGCACGCATATCTTCGGTTCCTGAGTTCATTTTCTGTCGGAGATCAGTTTTTTTCTCTGATAAAAACTATAAAATTCCAATCCTGAAAAAAATCACTCAAGAATTTTTGCTCGTCAGAACTTTCTTTTCTTACTTGATGATTACAATGAAGGAAAAAAAGCTGATCTTTCGGTCGATTTTTCTTCCCCCGAAAACTCGCCATCAAAAATGCGAGATACATACTATCACTTCCTCATGAAATCCCTAGAGCGACCTTTTGAGGAGCGGGGAAAAGCTTTTCTAGAAGAAGAAAAACGTCTTCTCATTCGGTCCAAGGCAGATCCTGAAATTTTACCATCATTATCACTTTTTCCAATATAATAAAGCTTTTTCTTTTTTATACTAAAATCTATGACTGTTGTAAAGAGAAGTTTTAGAAAAAAGACCCATTTAAAATAATGAGTCTTTCATATTTTTGGGAAAAGTAGTATTTATGCTCTTCTCTTTTGGAATACTCTATAACCAGCATAAAGCATCAATGTAATTCCAGCCATCACTAGAACATTTTCAACTGGTCCTGTTGCTGGAACATTTTTAATTTCAGGCTTTACATCTTCCTCTTCTCTTACATTAACGTTATATCTCTTTTCCTTTCCTTTACTATCTTTTGGAATGAAAGCAAAGATTAATTCCTTAGGCGTATTTTCATAGGTATATTCATATTTTTCTTGAGACATTGGTACTGTTGCAAGAGTTTGGTAATCTGCTGTTTTTTCGTCATATACCTTGATTTCAACATTTGAAGATCCTGGAACGGCAGTCCAAGTCAATGTTATAACATTTCCCTTTACTGAGTGAGAAATATCTGCCAAGTTCATATCTGCTCCTGCAGCGTTGTGTTCAACATCATCTGAACTCTCTAGTGCATTCAATGAGTTATCTGTACTTGCTACCTTTACTCCCTTAGGATTATCAAAGGTCGCACAAGCTTCTCCCTGAGCAAATCTCTGATTTCCAAAGTTAAAACAGAACTCTCTTGAATACTTTCCATAAAGAACGTTATCATCAACTGGGACAACTACTCCATAATAGGATGTCCCTGTGTTTACGGCACTTGCATCAATCCCAAGTTCTACCTCTCCTTCAATATCCATGGTAGAATATACAGGAAGTTCAAGTAGATCGTTTCTTAGGTTGAGAGAACGTTCACCAGAAACATAGTTCACCATTGGTTTATCTCACAGAAGAACATAGTATTCCCCCACAGCAGAATCCTTTGCATCCTTGAGAATTGGCGTTTTCACTAAAAATTTACTTGGATTTTTTGTTCCATTAGCATCTTGGAATCCCAAAGCTTGAATGTTTTGATTCTCGGTATATCCATATCCAAGATTCTGATCAAAAACCTGATTAAGATCAAGATTCGCTGCAGAGACCGCTCAAGCTCCTAATGCAAGCAAAGCAAGTAAAGAAAGCTGTTTTTTCATCGCAATAGCTTGTAATAAACTAAAATTTACATCTAAGTATATTCTAAATTTCCAAAAAAAGCAAAAAAAAATGGACTTTTTTTATAAAGAAGAAAAAGAGCATTGTTTTAAGGCTCTTTCTCCCAGTTAGTTTCTAGGGTATTATGAGATAGTAATAAGAGAGAAAAATTTGTAAAATTTTGATTTAAGATACTTTTAATGATTAAAGTCAGAATTTTTTTGGATATTTTTGCTTAAATGCTTCAGGTGAAAGAAACCTCTCTATAATAGCATCAAGTACAAGGTCAATGTTTTGTCCTGTTTTCCCTGATACGCAGATTACCTCTTCTTTTGGGATTCAAATGACATTTTCAATCTCCCCTGCTACTCTATCTGGATTTGCAGCTGGGAGATCAATTTTATTGAGAACGGGAATGATGTCTAATCCTTGATCCATCGCTTGATAGAGGGTCGAAAGTGTCTGAGCTTGGATTCCTTGACTTGCATCAATTAGAAGTATTGCCCCTTCTACTGCAGCAAGTGAACGAGAAACTTCATACTGAAAATCTACGTGTCCTGGCGTATCAATCAAATTAAGTTCATAGCCCTTCCGCTGCATTCTAGCTGGAGTCAGTTTAATAGTAATTCCTCTTTCTTGTTCAATATCCATACGGTCCAACATTTGAGAATGATCAACTTTTCTTACAGATCCTGTGATTTCCAGCATTCTGTCTGCTAGTGTAGACTTTCCATGATCAATGTGTGCAATAATACAAAAGTTTCTAATCTGTCCCATTAGCGTGAAGCAGGAAGGATAAAGTTTTTGGTATAATAGTGAATCCCTACTCAAAAGCAAGGAGAAGCCTGATTTTTCCCCTTACTAAAGTGTAAAGTTTATTTTTGTCGCATATTACCTGTTAAACTTTCCGAACACAAGAGCTTAATAAGCTCTAAAGCTTGCTTTTGATCAAGAAAGTATGGAGAAGTCGCAATCGTTACCATTTTCGCAGATGAGATCAGTTTTTTAGTATTTTTTATCGTTTGTTCAAAATTGTTTATCCCCATTTCTGGAGCTCGAAAATCCAAATCAATATCAAAAATATAATCTTTGTCTGGAATTTGGAATTGCAATAAAGCATATTCGCTCCTAATTTGTGAGACCTCTCAGATCAAGCCGGAATTGAGTGCAGGAAGGATAAAATTTCCCACATTGCAGCAGTTTTGGGTAAAAGAGCAGATAGATTCCCAGTTTTCCTGCTGAATGATAAAAGTATTGCTCTTCATATCAGAATGTTGATCAAGATGAAGAAGAAGGGTTCCTTTAGAAAGGTTGTTTTTGAAAAATTCACGATACCGGAAAGCTAAGGCGTGATTATGATTATCAAAAACATAGACAGGAACTCAGTTTTTTTCAAAAAAAAGAAAATTCTGAAGCCCGCTAAAAAGTTGAAGTTTCCCCTGATGATTAAGTTCTGCAAAGACTGGTTCGGCTCCAAGAGCGAGTCTTTCTAATCAATCCGAAATCAGCTGTGGAATCCATAATTTAGGGTTTGGTTTTCTTTCTTCATAGGCAAAAGCATTATTTCCTACTTGTTGTTCTACTCGAAAACCGCCATATTCTAACATTTTTCTGAATTAGTTGCTTAAAAAGGCTACTTTTTCGCTAAAAGAAGCACTTATCTCCCTTTATATCTGTTTTCTCCTGTTTTTCAAAAGATTATGTTGCTCGAGAGAAAACACCTTGTAAAAGCGAGGCGAATCTCTAGACTTGAGTCAGATTTATTTTTTATGTGCAAACGATGATGAGATTTTTGACTCAGATGAGCAAGCCTTCAAAATTTGGTTTAGCTGTTTTGGCGCTTGTAGTGCTGATTGGTGTTTTTCAGATTACTCTTGGATGAAGCCGATTTCATGAGAATGACCGTCCTCAAGTATTGGCAGGAGAGAAAATCTTATTGGATAAAACTCATCAGTTCAATATTGAGAGACTCGATAGGGAGCTTGCGGTTAACAAAATGAATGAAGATCAAATGCTAATGATCTGGAAAAGATTACCGCTCTTTAAGCCAATGATTGATAAAAAGCTAAAAGAAGCCTGACTTCCTTCTGATGTTTTTTATCTAGTTCTTGCGGAATCTGCTCTAAGAGAAACTGCAGTGAGTAGTGCGGGAGCTGCGGGGCTTTGGCAATTTATGCCCTCAACTGCAAAAGCATACAATTTGAGAGTTGACGACGCTATCGATGAAAGGTATCACGCAGAAAAAGCTACTGATGCGGCAATTCAATATCTCAAAAAATCTTATGAGAAGTTTTGAAACTGGACTCTTGCAATGGCTGCTTATAATAGAGGTTCATCTGGCATTTCTAGCGATATGGCTTATCAGTTTCAAAATAACTTCTATGATCTCTATCTGAATAATGAAACCTCAAGGTATATCTTCAGAATTATTGCTTTTAAAGAAATCTTTGAAAATCTTGGTACTTATTTTGATGTTACCAAATGGGGAAAACAATACAGTGTTCCAGAAACTACCGAAGTTCAAGTCGGAAAGACAGATAATCTTGCAGCTTGGGCAGCTAGTAAAGGATACACATATCTTGAAGTAAGAACACTTAATCCCCGAATTAGAAAAAATTCACTGCCAGAAGGTTGGTGGACCTTGAAAGTTTATAAGAGATAGGAGGTTCAGCTCTGCTGCATTATTGCGAGAGGCTACTTAATTCAAATCGTCTTGCTTCCTTTCTTTCAATTTGTTTGAGCAGCTCTCCAAGATGTTCTGAGAGTAAAGTAATTTCATCATAAGCTAAGTCTTTATTCTCAAGAAGCTTGGTAATCTCGAGCTTTTCTTCTTCTAAAACTTCTAAGTCCTTCATGAGCTGGGTTAATTCAAGCTTTTGTTCAAAGGAAAGAGATTCTTCAGCTGAAAAGGAAGAAGATAAGCCAGAAGAAGTAAGAGGAACCTGAGGTAGAATCTCAGGTTTTTTCTGTTCTTGGAGAGCATGCCATTCTGAGTATGTTCCCCAGAAATCTGTAATTTTTCCTTTACCTTCGAAAATGAATAAGTGATCTACGATCCTATCCATAAATGACCTATCGTGAGAAATAATAATTAAGCATCCCTGAAAAAGCGAAAGAAAGTCTTCTAAAATACTAATCGTCAAGAGGTCTAAGTCGTTTGTTGGCTCGTCAAGGATTAGAAAATTTGGATTTTTTATTAACACCTTCAAAAGTGCTAGCCTTCTTTTTTCTCCTCCACTGAGCTGATATGCGAAACGATACTGCTGAGAAGGAGGGAAAAGAAAATTTTCTAGAAGTTTGGTCGCAGAAAGTTTTTCACCATTCCCGAGGATAAGAAAAGGAGCCTCCTCCGTGATAATATCAATAACTCTCTTTTGAGGAGGGAATTCCTGCTCATCTTGCTGATATTCTCCAAAAACGACAGTTTTCCCGGTTTCTATAGTCCCTGCATCTGCTGAGATTTTTCAGAGGAGAAGCGAAATAAAGGTCGTTTTCCCAACGCCGTTTTTTCCAAGGATTCCAACCCTTTCGTGAAATTTGAAATCATAGGAAAAATCCTGAATGATAACTTTGGATCAGAATTGTTTTTTTAGATGTTTTACCTTGAGAATTTTTGTTCCCAATTTTCTTTCCTGCAAAGGAAGGTCAAGTTTATTACTTTCCGAATGAAGAAGCTCCTTTTGGTCATCATACCTCGACTCTAAGGTATAAAACTCTTTCTCTCTAAAATGCTGCTTTGTAGCTCTTGCTCTCGGAGATTTGCGGATCCAAGCAAGCTCTCTCTTGAGTAGCTGGCGCATCTTTTCTGTAGCGATCTGCTCATTTTGATGACGTTCGGCTTGTTTTCTGAGGAAGTATTCGTATTTCGCAGGATAGGAAAAAATTTTCCCTCTTTCTAATTCAAAAATCTGATCGCACACGTTTTCCAAAAAATATCTATCGTGCGTCACGAGAAATAGCGTACAATGCTCAGTTTTCAGATACTTTTCTAATCGCTCAATCATTTGTAAATCAAGATGATTGGTGGGTTCATCTAAAATCAAAAATCCAGGCTCATCAATCAGTGTCTTAGCAAGAGCCACCCTTTTTTTCTCTCATCAAGAAAGCGTTTTTATCTGCTGAGAAAGCAGGTCTTGTAGCTGAAGCTTTGCCAAAATCGTATCTACTTTTGATTGATAATCTCGAATCTGATGTTCATCTAAGTCTACCATTAGTTTAGTGAAAGACTCGCTATCTTTAGCTGGGTTGTGCAACAATTCTTCGTAGTCTCTGATAAGTTGAGCTGAACAATGATCTGTTTCAAACACCTCATCAGCAACGGTTTTCTCAGGATCGAGCAAAAATTCTTGAGAAAGTGATCAGATTTTTAATCCTTTTCTAAACTCGACTTTTCCATCGGTTGGATCTAGCTCTCAAAGCATGAGTTTGATTAAGGTTGTTTTTCCTCCTCCGTTTTTAGCTACAAGAGCGATTTTCTGCCCTTTTTCAATAGAAAAAGAAAGCTGATCAATAATTGCTCTGTCAGTATAGATTTTGGTCAGATTTTCAGCTTTAAAATATCGCATAAATAGTACTTTGGGATTAGAAACTTAAATGAAGAAAAGGTAGGTAGCCATATACCACTCAAAATATACTGATTATCGGTCTTTTTTCCAATATAAAATCACTTTGTAAATCTGGAGCAAATTGCTACACTGAGATCAGATTTTTGCTTTTATTGTATGAATGATGCTGTATTTTATTCCAACTCCGATCGGAAATAAGGAGGATATTACGCTTAGAGCTTTGAGACTCTTGAGAGAATTGCCTATTTTTATCTGTGAAGATACGAGAGTCACGAAAAAGCTCCTTTCTCTCTATGAAATTCCTTATGAAGGGAAACAGTTTTTTTCACTTACGAGCTTTACGAATAAAGGTAAAGTAGCATATTATCTAAATTTGATGAGAGAACATGACATCTGAATGCTCTCGGATGCTGGGACGCCAGGACTTTCAGATCCCGGAAAACTCCTTATCCAACTTTGTAATGAAGAAAATTTGCCTTATACGGTATTGCCAGGTGCGAATGCACTTATTCCTGCAGTAGTAGGGGCAGGGTTTGATACTTCTGCATTTCGCTATTTATGATTTCTTCCACAAAAAAAAGGTAGGCAAACAGCACTAAAAGAAGCGATAGCAAGCGAGCTTCCAAGCTTTTTTTATGAATCGGTTCACAGGCTAGAAAAGCTGGTTGTGGAACTAGATGCTCTCGGCTATCAGGGGAAAATCTGCATTGCAAGGGAAATCAGCAAACTCTTTGAACAGCATCTTACCTGTGATTTTGAAGAGCTAAAAGCAATGTTAGGCAAACATCAACTCCAAATCAAAGGAGAATTTGTAGTGGGCTTAATGCCAAAGTAGCAGATAAACTGAAGTATTTACTGATAAGACTATGCTGATTTAGGATTCTTTTTTGAGGAAACTAAAAAAAATCTGAAGTCAGATTTCTTTTTTTTCGGAAGCTTAGGGATTCGAACCCTAGATACAGTTGCCCGTATACATGTACTCCAAACATGCGCTTTCGACCACTCAGCCAAGCTTCCAACTCGCAAATTGCCCCGACCCGGAGTCGAACCGGGGTTACAATCTGTTTGGTGCGCTTCTGCTTTCCCTGTTTCTACTTGCTTAAATTAACAGTGTGAATTGTAAACAAAAAAAATAAAAAATCAAGTCATTTTTAAGAAACTTGATTTTTTCTTCTCTTAATTGGATTCTATAGCTCTCCAAAAACTTTTGCATGAGCCTCCTTGAGTTGATTGTTTGTGATGTGTGTGTATCTTTGGGTGGTAATGATCGATGAATGCCCTAGCATTTCTTGAACGACCCTTATATTAGCTCAGTTATTGAGGAGCTGAGTTGCATAAGAATGACGGAACATATGACATGTAATTCTCTTCCCAAAATTTAATTGATCGCTGTATTTTTGCATGATTCATACAATCGTTGACTTACTTAATCAGCGTCAAAAACCGTAGTCATCATGGCGAATAAAAAGCAAAGATCAATCTCCCTGCATTTTCCTTGTGTGTCCAGATCGTAGAGAAACAATGTTGCGTTCTCACCTAAATTTAACGTATTCTGCAGCCAGATTTTTAATCTCTTTTGTGATGTAAATTGGTCTAATTTTATCCCCCTTACCCATTATAGAGATCTCACGACCTTCTCATAAAATGTCTTTAACCTTGATTGATAATAATTCAGATAGTCTCATTCATGATATAAAAGCAAGTTTGCAGAATAAGAGATTTCTGAGCCTGTTTTCTTCATTTTTTTCATTTTTTTCGATATGAGTAAAGAGTTGAGATAACTCCTCCTTAGTTAGATATTCCATCTGAGGGAGGTGAACTTTAGGTATCTCAATTTTTAAGTAGCTAAGCCCAATATCGTGCCTAAAATTGAGGAACTTAAAAAATTTTTTGATGGAGATGATTTTTGATTGGACAGTTTTCTCTGATAAAAATTTGTTTTTCCCATAATATCTTGAGGTTTTTTGCGTTGGCGTCTTACGTAAGAGTTCGAGATATTCATAGATATTTTGGGTAGTTATTTCTTCAATGCGAACTACTTTTTTTGTCCTACAAAAACGCCAAAAAAGAACGAGATGAGACTTAAGACTAAAAACGGTTGTCTGTTTTTGTCCCAGTGCTAAAAAATGCGTAGCACAGTCGTCGATTAGGTTTTTTTCAAATTTCATTAAATAGAATAGAAGATAAAAACTGAGTGTTGCAGATTTATCTTCGTATGTAGTAAAGTATTTTATCCTAAAGAATAAAAAAGTCAAAAAATCAAGCTTTTTTATGTTTTTTAGCTTGAAAACTATAAAGAAAAGAATAAAATTTCGTTGAACATACGGAAAAAACAACACTCAGTAATTTACTTACTGAGAATTTTCAGTTATGAAAAAAAAGAAAACAGCCTGACCTACGAGAAGTAAGCTCGTCCAGAAACTGGATCAGGTTTTTTCTGTTTACATAAGACTCTTAGTAGCAGATAAGGACTGATACATAACCTGCCCTCTCTGCTGAGCTAGAGTCCACTGGACAAAGGCTCATAACATGCACTTCATCACACGCTCGGTGTATAAGTACAGACGAGATGAAAAGAACTGTCATGCGGGCTGTGTAAAGTGTAATGTTATACTACATGGTAACTACATCGTATATACAAGACGAATGCAGAGGAAGTATGGGGAAATTCTCGTGGATGAGATGATCAATGATAGGCAAATTATGAAAATCGCTACAAGCTCACTACAAGAGATGATAGACCACTATCAATCAATGGTAGATGAACTCAAGAGGACAAAAGGATTATAAAATCAGAATTTTTATCTTTTTTTAGAACAATGGCACGAATTTATAAAATCATAGAAAAAAGAAACCCTCACTATGAGGAGATGAAGGAGAGGTTTAAAGACAAACCAGAACTCATTGAAGAGCTAGCTCCTAGACTGTATGGACTCGCAGAGGTATACTTTACTGCAGGAAAAGATGAAGAACTAACAGATGAGCAGATAATTGAGAGAGCTGATGGGTATACTCAGAATATTTTATCTCGCTGGTATGGAAACCCTGAGAGCCTTATAGAAGACCTGAAAATGATGGTCAAATACCTCACCAAAAGACCACTTCCGTTGAATATAGATAAACGAGAAGAATAAATCAGATTTATTTTATTATTACATATACATCATGCTAACAGAAAAGAACATTACAGATCAGATTCAAAAAGTAGAATACAGTAAGCTCTGAGAGAAGACTGCTGTATGTTTAATCACGCTGAAGAATTGATTTGAGATCGTAGGGACAAGTGCCTGCATGAAGAAGGAGAACTACGATCAAGAAGTAGGGAATAAATTTGCCTACGAAAAAGCGATAGATAAGATACGAGAACTAGAGGGCTACAAGAATAGTTAGTCAGATTTATCTTTTTTATACCTCCAGCATGAAGGAACGGATAATCTCACTTCTTACGGAATGGATAGAAAATAAGTATGAGCGAATAAGATGCCTAGAATATGATAAGTCCTGGTATCAATATAATTACAGATACTGAAGCTATAAATCAGTCTCAGAGGAAGCTCTTAAGAGAGCTAATAGATGAATAAGAATCCTCCAAAAGGATGTCAAGAGATTAGAGAGGATTGCAGAATGGTTGCAGTAATCAGGTTTTATATTTTAAGAAAGTAGAAAATGTATTGAGAAGGAGTATATGAGAGACTCATTAACGAGGCTGTCAGAGAAGCCATAGTAGAACTGAAGTTAAAAATAAAGCCAGCCCCTAGGTGGATACCAAAGAGATTATGGCTTAAGTTAGCAAGCTTGTTTGTTAAGCTGGAAACCAAGTCTAGGAGCTAGCTATGAGAGAGTGTAGAGTTTTAGGATCAATTTAACTGATCATATTTTAATTTATTATTTGTTATAAAATGACAAAGAAAGAAAAACAACGAATCCCAACGGAACTACCTAAGTTCCAATATGGAGATCGCGTAGAAGTAATCTCTGATGGAGAAATTAGAGAAGGTCAAGGCATCTTACCTTATTTTTATGCTGGAAAATTTGGAGTAGTAAAAAACATTCTCCAGTATCCTGAAAGTCAAAGCTTCGGATTTGAGGTAGAGCTAGATGACAGAAAGAGGGTCATTATGAAAGAAAAAGAGCTTAAAAAAGCTGTTTCTAATCATCCATCAGACTATTCAAGTCAAGTTTAGCATTTTATCTTTTCTTTCTGTGAAATCTCAGATCACTTGGGACAAGTTGAATAAATCAAAACCCAGAGGCAGACCAGTCTCTGAGTTTCCTCAGTGGTCAGAAAAAAAGAGTGAAGAGTATAAGAGTAAAATCATCGTGGGCAAAATAATCGAATTTGTCCAGAAAGACGGAAAGAATAAAAACACTAAGGAATACTCCTTGTTTGATTTCTATAAATTCATCGTCAAGAATATACCAAACGAATACTTTTAATTTATTATTTACACAAGAATGACACCAGAATTAGAACAACTTCATTCAGATTTTCAATCTGTTTTTGGGAAGGAAGTGCCAAGCAACAAAAAAAATGATGCAGAATGGATACAAAAAAAGATCGCCGAAGCAAACGAAATCAATACTCAAGATCAAACAGATCCAGTAGCAAATTCAGAAAATACTGAGCAACAGGAAACTGTAGAACAAGAACAAGAGCAAGAGCCTGAGCTTCAGAATCAAGATGAAGAAGAATATAGCTCAGAGGAAGATACACAACTGAGCTATGAGGCAAAAAAGATTTCAAAAATAAAATATCCTCTTTAATATCTAATTAGTAAAGAATGGCAACAAAGAGAACTCGAACAGAAAAAAAGAGACCAAGAGCAGATAGCCAAAAGAACAAAAAGGCTGTCGCTCTTGCCGTTGCCAAGAATCCAATGGCAAGCACAAGAGAACTCGCAAAGATTGCAGGTGTGAGCAAAGGAACGGTTAATAATAAGTTGGGCGAACTTAGACAAGTCAAAGATGAAGCCATATTGTGAATTTGTGATAAAGATATGGAAATTGTTACCAAAGCTCAGGAAGAGATTTTGAGGAGGCTCAATAGCCAAAAAGAACTCACAAAAATGAAAACTTCTGAAATCTCCTCAGTAGCAGAAGCAAGCACAAAGAGATATACAATCTTTAGAGGAAATGCTACAGATAAAAATTGAGGATTGAACAATCCCTACAGTGAGCTGAGTGATGATGAATTGCTCGCAATGATTAAGGACTAGTTTTTTATATGAATTTCTGATATGGGAATTGATGAGGTAAAAAAAGAACTCGCAAGGAGAGAACTCGCAAGGAGAGATTTTTTGCACTACCTCAGATATGTATTCCCCGCTTTCCAGGATGTTGCCTATCAATGAAGTCCTGATCATAAAGTCCATCATAGAATCACTGAGGCACTAATGAGAGTAGCAAGAGGAGAATGCAAGAGACTGATGATTTTTTGTCCTCCAAGAATGGGAAAATCAACAGACGTTACTCAATACTTCCCGAGCTGGATGTTGGGAAATAATCCAAATCTTAACTTCATTATCTCATCATACTCTGCTGATCTAGCAAATGATTTTTGAAGAAAAACAAAAAGAATTTGTGAAAGTCAGCTTTTTAAGAATGTTTTTCCAAATTTTGAATTTGCGAAAGATAAAAGAGAGTGAGGGAATTGGGAGACTGCTCAAGGAGGAGGTTACTACTCAGTCGGAGTTGGAGGATCAATCACAGGTAAGGGATCAAATATCCTTTTAATTGATGATCCAGTGAAAGATAGAGAGCAAGCGAACTCAGCAACCATTCAGGCAAAAGTAATTGATCGATATGACTCTGTGGCATTAACCAGGCTACAAGACCAGAATTCTGCAATCATCGTGATTATGACCAGATGGAATATCAACGACCTCTGATGATACTTAGAGAGAGAAGAAAAGAACTGAGGAGATATTCGAGAGAAAGTAGTTATTCAAGCAATAGATGATAATGGCAACGAAATTATCCGACCCTGAAAGCGAGACAAAGGTTATTTTACGGCACAAAAGAATAAAATGCTTAAAGAAAACCGATATGCACTCTATCAACAAGATCCTATCAATGCAACAAATGGGATCTTTAAGAGAGGAATTTTTCAGTATTTTAAGCTTTCGGATTTTGAGAGAGCTGATGGAATACTAAAAAAATCTGATTTAACCTGTGGAATTATGATAGATCCAGCATTCTCAAGTAGTAAGAATTCTGATGACGCCACGGTTGGACTCTACGGGAAACATAAAATCACTAAAAATCTCTATCAATTGGATCTCTATGCTGATACTTCAGCACCAAGTGCAACGATCTCAGCGACCATAAGTATGGTGAACAAAGCTAAAGCAATGGGATACAAAGTCAGCTTTATTCATTGTGAGAGCGCAAAAATCAATAGAGAGCAGACGAAATTTATTCAAGACCTCAAAAAAGAGCTTATCTATCAAGATATTCAGATTCCTTTCTATACTACCGAAGCTAAGGGTAAAAAAGAGGACAGGATCAAGCAAGAATGTGAGCCACCGATGACCGCTGGAGGATTCTTTATGAGAGGTGATGGGAATACCGAAGTCCAACTCAAACAAGAACAACAATTCCTGGATTTCCCAAACTGAAAGCACGATGATATTATCGATAATGTCGCTCAAGCCTATATGTATTTTAGGAAAATTGAAAAAAGGGCTGGAGAAAGTACGGATAGATATTCTGGAGTTGTGAACGTAAATAGAGATTCAGAGCTCTACTAAAAACAACAATAAAAAAATCATAAATCAGAGAAATATAGCACAACAACCAATAAAACTCAAACTATTTTTCTGCACAGAAAGGCAAACTGAGTATAAAGGGGGAAATTTATATTTCCTCTTTTTGTTATGATTAAAAATCGAGAGAAGCTTGCAGAAAAAAAGAATGCAGAAGAAAGAATGCGAACTGAGGGGCTTAGGCTTCAAGTAATTAGAGAATATGAGATGGGGGCAAGTTATGTGAAGCCAAAAAGAGACCAATATGCAGAGAGGATCGAGAAGTGGAATCCTCAGAGGAAGAAAAAAAAGAAAATCCTGAATATCAACATGATCTCTGAGGCGATAGATGTGGCGGTTTCGGTTTCTTATTCTGATGCTCTTAGTGTCAGATTTGTGTCTCGTGATGGATTCCTCCACCAAGAACAGGCTGACAAGCTCCAGTATGTCGCCGAATTTGACTCTCAAGAAGCTCACTATCAGCAGATTAAGTACCAATCAGAGCGAGATAGACACTTTTATGGAGTATCAATCAGATTCAACGAATGATGGGAAGATACAAAAAAACATCCTATCTTTACCGTGATTGATCCAATGACCTGGATTCCTGATCCGCTACCTTCACAGACAGGGAAATTTAATGGTCAAAACTATAGATTTCACTGATTCAGTATGCAAACGAGCATTTGGGATATGATCTGAGCTAAAAAAGCTGATGGAAGTTGTCTCTATAAGAAGTCAGACCTTAATAATCTTATAGCAAACTACACAAAAGAGCAAAGAGAATCTTCGAGAGCAAGAAATTCTGCTTGGAACTATAATCAAGTGAATGTAGATACCTTAACAGGGAATTTCAGTCTAGGGTTGTATTATCACTTTACAATTTATGAGGGCAAGAAAGTCTTCACAGTGCGAGATGCAGAACGCAAGACTTTGCTTAGATATGTAGAACTCCAAGCTGTGACTAAGGAGGAAAAGAAGAATGAAAGGCTGATACCTCGACCAATCACCTTGACGTACTTTAAGCCTCGTAGGGGTGATGCATTCGGAGAGAGCTTCTGTGATTTGCTTGAGGATAAGCAAAACGGAAAATCAATCCTTGCGAATCTCTCAATAATTAAGGCGCAGAAAGAAGCCAGAGGAGGAAAGTTTATCGTGAACAGTAGGCTGATCACAAACAAAGAAGCAGTTCTGAATTCTTCTCCTTATGAAAGCTATATTTTCACAACACAAGACACAGAGAATGAGAATCTGAGCAATGCGATGTTTGAGCTACCTCAGAGTCAGATCAAATCTGATGTATGGTCAATGCTGAACTTTATTGACAATGAGGCTAAAAGCGCAACCGCTCAAGATGAGCTCCAGAGAGGAATCATCCCTGACAAGAGCATGACGAAAGCTGAAGCTCAGCAAGCTCAAGCGAATAACAATATCAGATCTCTCCTGAAAAATACCGTAGCTTCACGAGGAGAAAAAGACTTTTGGTTTCTTTGGTGGAGGTGCTATCTGCAATACTTCGCACAATCAGATGATAAGTTTATAGCACTGAATAACAATTTTGAGTATGCAGGAGAGACAATCAAAAAAGATGAATTCTTAACAGGTCTCGATCCTCATATTATGATTGGAACAAGGTCAGAGCTTGATTCTGTCGATGACCAGCAAGCTCAATTCTTCACCTTGCAAGTGCTCCCAATGCTCCAGAATCCTATGCTCTCTGAGGCAAGTAAAAAAATGATGATGAGGCACTGGCTAAAGCTCAATAAGATGAAACCAAATCAGATTTATGCTTTTATTCCCTTAGATGCCACAGAAAGGAAATGTAAGGCTTATGTAGAGATGGTAAACGCTGATATTATGCCTGAGTCAATCTTTGCTGATCCTAGCGTAGATTATCAAACCCTTTGGATCTATATGCAGAATGCAAAAAGTGGAGAAATGAAAGAAGCTGTCCTTGGAGCATTATCGCAAATACTGATCCAATCAGGGACTATGCAAACTACTGAGCAATACGGAGCAATAGCGAATAGTGCAAGCAATATCGCACTCGCTCAAGGAATGCAAGGAATGAGGCAAGGACTCCAAACTAGACAAGATGTTTTACCTGCTAAATAAAGATGAATAAGAAATCTGTACTCAAAAAGCTCTGAGACTGGAATGTTATGACTAATACTGAGTCTCGAAAAGTCTTTATCTGAGCAATTAAGGAATATAGAGTCAAAAAACTCAATGAGCTTCTTAACTCAGAGGGTAATGATGAAGCGCTCAAAGAAGTCATTAAGCTCATGAACTTTATCATAAAAAAGCCTGAGGAAATCAGTGCTAATCTCAATGGCGAACTCTTCGTCGCCAAGAAGAACGAAGAGATAGAAAAAGAGTTTGAAAGCTACTATTAGAACAATCTGGCATTTGTGTAGCTCACAATGTAAGCCCATCCTTGCCCTTTGGTGATTCAGCTTAGGAATCACTTTCGCGGTTGGTATCCGCTTTTATCACTTACCACACTATAACAATGGATGAAAAAGAATTGCATGATCAGGAAATGGATCAAGAAATGGAAAATGAAGATCAGGAACAGGATGAGCCTGATTACAAGGCTTTATCTGAGCAGTATAAGGCACAAGCCGAGAAAGAAAAAGCTCGTGCTGATAAATACGAGTGAAGATATAAGTCTCTAAAAAAACAGGATAAACCTGAATCTAAGTCAGAGTTTTCTGAAGAAGATGTCGAGGAAAGAATCTACTCAAAAGTAGAGTTCTACAATCAACATCAAGGAGCAAGTGAATACAAGAATGATATTGAGAATCTTGTTAAGACTAAAGGTCTTGATAGAGAGGACGCAATGAAGCTTGTATTAGCTCAAAAAGCACCAGAGAAGTTGGTTGATCCAGCATATTTCAACAAAAAAGATGCTGGTTCTACTTCTATGCAAGGGACTACCCCAACTTCTAAGGCTCCAACAGTTGAGAATATCCTTAAGGCAAAAAGCGTTAAGGAACTCGACGAAATGGTAGGGATTGCATAATTTTATTCTTTATACTACTTAAACAATGGCAGGAACTAACGACGTACAAGCATTTATTCCATCAATTTGGTCAAAAAGGACTCAATTGATGAAAAGAGAGAAATCAATTATTTACGCTATTGCAAACTTTGAGGAAAGAGCAGGTTTGCAATTTGGAGCAGAGGTATTTAGACCTTTTATGATGGATGATCTCGAGATCAATCAATACACAAGAGGTAAGGATGGGAAAGAGCAGACACTCAAGACTATGCCTGAAAAACTCTTGATTGATCAAGAGAACGAAATCACAGTGTATATTGATACCAACGATCAAATCCAATCAAAGTACTCTATCGAAAAAGAATTTTCAGACAGAGCATCTAATTTGCTTGTAGCAAATGAAGATGGAAGATTTCTTGAGGAGATCCTCTTTGCTGAGTATTCGCTTGATGGTGGAGTTGTTAACAAAACTAATGTTGCAGATCTCCTTTCTGAGTCTAAAGACGAGCTCTCTATCAATAATGTAGAAGATAAGGAACTTATCGTAGTTGGACCATCAAAGCTAATGGGAGCAATTGAGCTCAATGCAATCAAGGATGGATTTAATCTTGCAGATTCTACTCTTAAGAATGGATTTGCTGGGACATTCTTGGGGCTTGAGTGTTACAAGAACAACCACTTGCCTCACACTACTAAGCTTAGCAAAGTACCAGCTGATGGAGATTCAATTAAAATCAATGGGGTTAAGATTACTTTCAAGACAACTCCTGTGGCTGCTGGAGATGTAAAAGTAGGAGCAAACTTACAAGCTTCTATTGAAAATGCAAACAAAGTACTTCTTGGAGCAACTGAGGATTCAGCAACAGGTAAAGCACTTGCTGATGAAGACAGAAAAAAGGTAAAGAGAGTAAGAGCAAAGATTATCCTTACTGGGGCAAGCGAAGCTCATGTCTTTACTTCTGGGAAGGCGAGAATTGAACTTACAGGGTTTGCTTCTGAAAAGACCTATGTATATGCAATGGTTGGACAGAAGAAGACTATTGATTGCGTAATTCAAGATATGCCAGAAATTCAGCAGAATAAGGCTGAGAAAAGAAAGGGATACTTTTATATGGCAACGGATCTCTTTGGGGTTAAAGCTTTCAAAGAGGGAAGAGAAAGAATGCTTAAACTTAAGATGGAAGCCTAAGTATCGCTACCGTCCGCCTAGGTGGGCGGTAGACTTATTCAGGCTTTTAAAACTTGTAAATTGAAAGAATGACTATAAAAACCGAGTTTCTTGATCTGGTTAGAGAGGAAACAGAACTTAATCTCCAACAATGCTCAGATGCTCTACTCATGACGTATCTAAATCAAGTGAGAGATGATATTTTTAGTTTTGCTTCAACCTTCAACGGGAATCATTTTACGTGGAATTACTGGATCACTGACCTCATAGCATGACAGGCAGAATACACCATGGCAGAAAAGGCTCAAGATACAAAGTATGATATGAAAAAAATCCTGTCTGTTTACAGGAAGAATGAAAATAACGAATGGAATAAGCTAAAATCCTATGATTTGGAGGCTCTTCCTCATTCCCTATCTGAACTGGAGCAGAATTGACCAGATTTTTATTTTCGTGCCGATACGAACAGCATTTTCCTCTATCCGATTCCTCTAAAAACCATCAAAGATGGACTCAAGGTTGTTGGAAGCTATTTACCAAAAGTAGTGAATGCTGAAGATACGATGGATGCACTCCTAATTCCTAGAGAGTATCAGACACTCCTCTGGGTTGGATTGACTGCAAAAATCTATCAATACAAAAAACAATATGACGAGAGAAATCTTGCTCTCCAAAACTACGAGAACGAAAAGCTGAAATTCAAAGCTACGAGTGGAGAAATGCAACAGATCTATTATGCAAATGCTAATGATTTATCTGAATTTGAATAAAAAATGGCTAAAAAAGTGTATATGATCTCAAGTATTAACAACGGAATAGCAAACAATAGGAAACTCTGCAAACCCTGACAGGTAGGAGACCTAGAGAATATTGACTTAACAGCAGATGTGATGAGTATAAAACTCGCAGGGAAAGATTCAGAAAATATTATCAATTATAGAGGAGAACAAGGGAAAATTGTTCACAGTGTATGAGATACTTATCTCACAAGAGACGGACACTTTGAGGATGTTTATATTTATAACGAAGCTGCTCTCAAAAGAAAAACGCCGAGAGATATTAGTTATACACAATATGTCCTTCCGAAACAAGTCACTAAGCATCTCAAAGGAGATGCTGGGTGATTTTTTGAAACAAACGGGAGATGAGAAACTACTGTTTATGGAGCTTTAAGTTTTTCAAATAAATCACAATACTTTGATCTGACCTATACAGTGCTTGCTACCAATACTTTTTTGCATCTTGTAAAATCAAGGATAAGTGCGAGATCAAACGTAATTCCCAGCGAAGTAAGAGATGGAGCTTATACAAACTATTGAGGACGGACATATCAGACCGACGGTTATAAACACACCGCGAGAGCCACAACATGAATTGCAAGGAGGGTAAACGAACTTTTCCATTCCTTTCAGTTTGAATTTGCCTTTATGGAGTGGACTAAAGGGACAGTACAAGTTTTGATAGAATATACAGGGGAAGAAAAGACCAAAAATGGGAAAAACTACGACTCTGTAGAGACGACTAAGACTTATACCGAGACTCTTAGAGCTGAGAGGAATGGGCTCATAAATAAAATCTGAATACAAGGGCAGACAAAGGAAGGGGTTACAATTAAGTTCACTCCTAGTGCTGATTTTGATGGGAAATTGGTTTATAATGGACTATATTCATGGTGAGATACTTTAACCAATCCAAAGTACTTTGAGGAGCTTTATGGAGTCAGAATCAATTACCATAAAATCAAATCAGCAGATAAGCATCCTATGATCATTATCAGTAACAAGCTCTATGTAGGATGTTGAGATAAAATTGAAGTTTATATGATCAACCAGGAAGAAGATGATAGGAATGGGAATATCCAGTTAGTTGAGGATGTGGATGTAAGATTATGAGTTGGGATGAAGATTATCTCTCTTACGCAGTACGGAAATTATCTCATGATCTATGCGAATGGAAGAGAAGATGCCTATCAAATTGTCTCAGATGGTTCAGGTTCTGCCACTGAGAGTACTTTTATCTGGAAAGGAATGCAATTTGTCTCAGTATCAAATGATGGAGGACAAGACTTTGTTATTACAGAGAGGCAAAATAGTTACCAATTTTGGGCAGTAAGTGGGACAAATAAGCAGATACTTTTTAGCTCTGAGAGAATTTTTACTCAGAAAAATATGAGAGAGAAAGAAAAGTATCTGTTTGGCTTCTGAGGAGAGGCAAACTACTCAAGAATGGGAAGGTACCTCATATCAAGTAAAGAAAATGAGGTATATCTGTTTGATGGGCTTAAGAATTGAGTAAGAGGGATGAGTAGGTTTACCCTTGATCAAGGAGTAAAAATTTCACATATCCACGAAGAAAATCAGGACTCAATTCTCTGTTGCACCACTAATAGGGCAACAAAAATCAATAGTATGCAGAGGATCCAGCTCAATAATGATTACAATACATGCTTTGATGGAAGTATCACACTCCCTACTCTCGTAAGTCCAATAGAAGAAGGACAGCTAGAAAGCATCAGATTTTGAGCTTATTTGCCTAACGAAAAGAGTAAAATAGAGCTTCGAGCGATGATTAACTCAAAAGACTACTTCACTTTTGAGGTAAAGAATGCTGAGGGGAAGAATTTTTCAAAATGCAAACTTCAAAATCAGCAAGGAGCATGGCACTTAGAACTGATAGATTCAAAACTTGATAATGGTGTCGGGTATGTAAGCTTTAGAACTGTTGGAGAGCTTGAGAAAGTAAAAACCTTTAATGCCGATACAACTCTCCTAGATATGAATGGATGAACATTATTAGAAAGCAACAATATTCTCAGCTATGACCACTATATCAAAGTTGAAGAAATCACTAGAGCCAACCAAAAAACCTGAACAAAAGAGCTCTGATACACAACGACCGTTGACTTAAGAAAACTTAATGCAAAAATCTGAACTTTTAACGAGGTGAGTATAAAGCTCAAGCTCAGTGCTGGTGGGACTGCTGAGAGTCCAAGGGTCGCAGGACCAATCGTTTATTTCTATACAACAAAAGAAAATGGCTAACGAAGAAACCCAAATCCTAGACTATCACGAGAGGAACAATAGATTTTGATGACACCCTGAATATATGGAAGATTACTGGCTCAAGAATGGCTATGAATGGAGAGATGGACTACTGCAACCTAAAATAGATCCAAGGAGTTTGAACGATGGTTGAATACCAACGAATGATTATAATATGCCCGCACAACCAGGACTTAATATGATGCCACTCAATGCGAGATTGCAGGTAGGGAGTAAGTATTTGGATCAATGTAATATATCTGGGTATGTGGTGAAGAATTTTGATGGAAGCTGGGGTAATTTAACAAAAATTCCTCTCATCAATAAGATTAACTCACTAGATGAGATCAATCATGAATGGCATCAGGGGAATATGAAAGACTTTGCGACTAAGAAATTTTTTGAGAAAGAGGTAGTTGGAAAGATTGATTGGCAGGTTAAAGATTGATATTTGGAGATTCCGCAAGACTGAAACTACATAATCTACTATGTTGTTGAATTTTTTCATGATAAGAATCGGGATCCTGTAGATTCTCACTATAAAATGTATGCTGAGATTACACTTCATAATAATAGTGGGATACCAGATTATATAGGGAGAGAATCTAAATATACAATAACATTACCAGATTACATATCTTGAACAATTGTGAGCAATATAAAGGCAAGACAAAAAATCTGAATCAGTTGCATGTTGAAAGCGGTAGATAATTGTTTTGTCAGAACTTTTATGTCGGTGATAAAACTCTCCCAATAAAATCAACTTGACTTTTAATATGGAAAGGGTATTAGAGAGGATGTTTTATCTCCTTTCTTTTTTTAATGAAAAAGCTTTTTATACTACTAGCATTTGCTCTTGGGGCTTTCTGATTTTCTGCATTTTGAATGAAATGAGTCTGTATAATTGGAGACATAGAGTCTTTATGCCTTTGTAAGGTTAACGGACAAAATAAAAATACGCCTGTAGACCAAGAGGCTTATAATTCTTGTATCACCGAAGAAAAAGCAAAGCGTAATCAAAAAACACCAGAGGAGAAAAAAGCTGAGCTTGAGGAGAAGGTAAAAAATCTTGAAGAAAAGACTGAAACTGTAGTTGAGAGAGTAGAAACAGTAGTAGAAAAAGTGGAGAAAGTAGTTGAGAAGAAAATGACACAAGAAGAAGCTCAGGCATATGCAGAACTTAAAGAGAAATCTGCGGTTATTGATGGGCTTTTTGAAGCTATTAAAACTAAAGATAAAGCAACTCAAGAACAAATAAAAAGTGTTGTTGAAGCATTCAAAAAAAGTAGTGATCAGTATACTAAGAATATCGGATTTTACTTGGGGTATTTGATGAGATAATGGGAAGAAAAATCAGGATTCTTCTCTTTTTTCTTATATTCCTTGCTGGATGCTCAAATGGTAAAATTGAAGAAAAAAGAGATACAACGATTGTCGATTTATTAAACGATGGAAACAGTATTATCTCTTGTGATTTAAACATATGACCAACAATAACAGCAGAAGAAGATGAAAATTGAAGGATGGATACGCTATCATTTAATGGAAATCAGAAAGAATCAAAACCACTTCAAATTATCATTACAGATATAGCATCTGAAGAACCAAAACTTAAGGGGAATGCTGATGAAGTGGAGCTAATAAAACTTCCTACAGGGAATCCTGATACTGTTTATATGCTTGAAGAAACTGGTTTTTGAAATATTACTTTGTGGACATTTTTCAAGAAGCAAAAGATGCTGGTGATGAGTAAACAGTACTATAACTCATTAGGTGGGTATATCTATGCAACACAGATGGCATGATTCTGTAAGTAGTTTTTTTTATTCTTTATAATTGAGGAATGCCTTGGAGGTTTTTAATTTTAATCGGTCTATCAATTTATGCTTTTGCCAATGATAATGTCTGAACTGGGGTATTCTTGATATTTCTAGCGATTGTTCTTGAAATTTATTTTTTTAGAACTAGCTATTTGTGATGGATAAATGAGCAAAAAGGCTTAAGTTGAAAACCATACAGTATAAAAATAAGTGTAAATATAGCAGAATTGCTAGAGAATCCCGACTCCATCTTGTCTGAGTATTTGGATAATCTGTTTGGTGAGTATTGACATATGGAATTAAAAAAGCGAGAAAAACTCGTCAAGCCAGAACGATATTTGGATTGATTAAGTAGATCAAAAATAGAAAGTTCTTTACTAGAGAATGCAAAGGACCTCTGAAAGCCTCAATTTAATTTTGTTGTTCAAGGGAATTGTGTATTTTTTAATAATAAGCCCGTTAGTAACGGAAAAATTTACGGAGAAATTTTTATCCCATATGATAAAGGATATGATGGACTAATGTTTATTACAAGAGGACTATCTGTCAGAGCGTTTGTTGTCAATGGTATACTAAAAGTTCAGATAGGGAGGATGATTAAACAAAAAGAAGAATGGCTTGACATCATCCAAACAAGTTTAGCGAGTAAAGTTTTATTATATCCGATGACGGCGGAAGAGTTTAATGCAAACGATGACATTTTATTTGAGGATTGAGAAAAGGAATATCACAAATATTTTAAGGGGTTCATAGGATGATTTAAAAGGGGGATTTTCAAATATCCAGCCTATTATAGATTATATGGATACTTGAAAATGATAAGAAGTATTGGTAAGCAAAAGTCTATCGGTAAATATTTGGATTTTTTTAGGGCAGAAGCCCTAAAATTAGAAAAGAAATATAATATTGAGTATGAATATGCTCCATGCAATAATTATGCTTCAATATTTTTTGATTCCAGTAGAGATCAATCATTTACAAGGTTTTGATGAGTAGAAAAACTTTAATTTATTCTCTACCAATGATTTATGCAGATGTAGGTATGGATACAATTTCGACGGAGAGAAGTGTGTTTAATTAGAACTAAAAAATAAACTTCTAAGACGATCTAAAGAACGGATCGTCTTTTTTTTATTTTTAAAAACAAGAAAACCCTGCACAAGAAGCCATAAAATCAGTTTATTTTTCTGCACGAAAAAGTTTTTTAGCTATAAGAGGAGGAAATTTATCCATCCATCTATAAATGTCCACAGAGCGAAAGAATGGGAGAGTAAAGAACTGACCTTATAGTAGGGAGTATCTTCTTGCTTCGCTTTTGGTAATTCTCACCGATCCGAAAGACAACAAAGCACTGATGATTAAGTGCGATCCTGCCTATCCTTGAGCAACCAACCGATCCGAAAGACAGCAAGGCTCTAATACTGTTCGGAGAAGCAGATAATTTAATTCCCCTTCTTAGTAAAAATGACACCAATTCCAACTAAACCCATCAATGAACTAGAGAAAAAAACCTGAGTAAGCAATCAGGATTCTATTTTGATTGTAGATTCTGTTACTGGAGAAGCAAGACTAGCAGATAAAGAAGAACTAAGAGGAGTAGGAATTAGTAATATCACAAAGTCTAAAAACTGAAAAACAACAACTATCACAATCCATACTACAGATGGGGGCAGTTATCAAGTCCAGCTAGAAGATGGAGGCGATGGATATACTCCAGAGTTTAAACTTGAAGGAACAGTCTTTAAGTGGAAGTTCCCAAATGAGAGTCAATGGACTACACTCTTTGATACAACTTCATTCAAGGGGGAAAAGGGAAATGATGGGGTATGAATCTCTGAGATTACACATACTAAGAGAGGTAAAACTACGACCATAACTATCAAAAAAACAGACGGCAGTTCTCATCCTTTCAGTGTTGAGGATGGAGAAAAAGGAAACGATGGTAAAAACCCAGAGTTCCAACTCTGATCGACCCACCTCCAGTGGAGACTCCAAGGAGAGAGTCAGCGAAAGAATCTCATTCCCAAAGAACAGATCAGAGGGGCTACTATTACTAATGTGGCAAAAACTGCGGGGAATGGGGCTCCATGAACTACCGATACTTATACGATCACTCTTTCAGACGATCAGACTTTCAGTTTCTCAGTGTATAACGGAAGAGATGGTAGCGGATCAGGAGATATGCTCAAAGCGAACAATCTCTCGGACGTGGTAGATAAAGCACAAGCAAGGGCTAATCTCGAGCTGTATTCAATAGCTCAAATAACTGCCCTTCTCCAGAATATGCTCACCTCTGAGAATCTTAATGCTGTTGCTAGAACATTAGCGGGGCTAACTGTAGTCCCATGAAATGGGTCCGTTAGTGCTTCTCTTTTCCTTAGGCATCCAAACGGAAGAACTTATGAATTTTTCTCAGATGGTAGCTGAGCTTTTGGAGTTTGGGATAAAACAAGTGATCAGAATGTTCTAAAGATGACCACAGAAGAAGCTATCTTTTTCAGAAGGATTAATGCCAACAATATGCGCGTTACTAAGGTCGCTAATCCTGTGGATTGACAAGATGCAGTCAATAAGCAGTATCTTGAATCGCAAAAGAACCAAGCGAACGGGATTGCTGGACTGGATAATAATAAGAAATTGGATCCATCGGTAATTCCACTGATTGCAACCACCGAAACGGTAACGGTAGCGAATGAGGCAGCAAGGTTGAATTTAACGACAGCACAGGTCCAGAGAGGAGATTATGTCGTTCAAACCGATACTGGAGCGAAGTTTATTCTAAGCGGAAATAATCCCAAGAATAAAAATGATTGGATACCAATGTCGGATACCACTCCTGATCGGAGTCAAATCGCTAATAAACCTTCTTCATTCGCTCCTTCTCCACATTCACACACAAAAGCAAGTGTTGGATTAAGTAAGGTAGATAACACCAGTGATGCTGAAAAGAACTCAGCTACTGCAACCTTGACGAATAAGACGATCAATGGAGATAATAATACGATCACTAAAGTAAATAGTCTCAAAAACCAGAATGGAAATACGGAGATTAAGACTCGAGCAGGGACGAAGGCTCAGCATACTGCGGTAGGAACTAAGGATGCGAATACGCTTTACCTTGTAACCGAGAATTAATGCTACAACTAGGAGACAAAAAGATTTCTGAACTCTACCTCGGGGAAAAGAAAGTGTCTGAAGTTTATTTAGGGGAGGAGAAGATACGACCGGTTGGATGATTTACACTGACGGCGAATACGATTGCGTATTATCCGTTAAGTTCAGATAGTAAGGACTATAGCGGAAAAGGGAAACACGGGACGATTTCTGGAGCTGTATCGTTTGTTGGGGGGAGGTCTGTGTTTAATGGATGATATATTAACCCAGGATTCCAATTCCCTACAGAGAGTTTTACGCTTTCCCTCTGGGCAAAGACTGATAGAGAATACAAAGTATCTAATAATACCCGAGCATCATTAGTAGGGAAATACTGGTGAGGATTAGGGGGAGGTCAAGAAGCTTTTATTGTAGGGGTATCGTGTAGGACTTCGGACAATCAGCTTGCTGGGGTTTGGACAAGAGATAAAAACTGAACTGCCAGTTCCGATAATGTGGAACACACGAGGATGAATATCGGAGAACGGCACCACATTCTTGTTACCTATGATAAACCATCAAGGAATCTTTCGCTAGCTACAGATTGATTGAAAAGAATCACGGAGAATAGGAACTTTGTGGCTCAGGAGGATGGAAATCCCTATATTTGAGCTATGTATCACAGAACTAGTTGAGCAACAAACCGCTTTTACGGCTCGATCCAAGAAGTCATCTTTGAAAACAAGTCTTGGTCAGATCAAGAGATTGCATCCTACTACAATTCCACCAAGTGACAATTCTGATTATAACCTTTTATTTTATTGTTTTATACAACCAATGAACAACTTGAACTTTTTAGATGCATTGAAAGGAATCTTTGGCGGAGTCGCTCTTGCTGGGGTTTTGAATTATTTCGGGATGAGCGGGGAGATGCTCTCAATCTTATCAATCTTACTGTTAGTAGACTTTATCTTTGGAGTGCTTAGGGCAAAATACAAAAAAGAGTCTATAACTAGTGATAATATGAGTAGATGAGTGATTAGAAAGGCGACGAGATGGTGTATCCCTTTTATTGTTGTAGCAATTATCAGAGGAGCAGGTATTGATGGAGCTGAGAGACTTTCTAATACGATTATGGGGATTCTGATCGTTGCTGAGGGATATTCTATTCTCAGACATATTTATAACATAAACACAAATAAGGATTTGCCTGAAATTGATGTTTTTGATGCGTTAATGAAGAAATTGACTGAATTTTTGATCAGTAAATGACCAAAATTTCCCGGAGAAAAATCCTCCAAGAGCGATACCGAGAGACCAGAGTAAAGGTCTGACTTTAGACCTTTTTTCTGGAGACAGGCAGATGGTTTTACCCTAGTTCCTATTGTTATGAATCGAATAAAAATAGCGATACTCATAGCCTTATTGGCATTTTATTTCTTATGTTTTTAGAATGCTAGAAACTGAAAAAATCCAGAGAATAAACCAGCAATTAGAGGAAGTCAACAAAAAGATTAACGCTAATTCTGGGATATGAGAGAAGGCTCTGCAAGAACTAAGACTGCTCTGCATGGAGAAAGCGAGACTAATTGGAATCCTTAACTACCTTCAACATTAGATTTTATCTTTTTTATACTTAGAAAAATGACAGAAAAAGAACTCATCATCACTCACGATACAGAGTGATTCACCGCAGATGATTACCTCCACATCCCTAGTTATGCAACGGCAATGGAAGAGTATGGCGAAGCCAACCTCCCTGAAAAGATTGTGTATAATAACACTCCAATCCTAGACCAGTGATCAGAGTGAGCCTGCTCAGTCTTTGGAATAACCAAAGCCGAAAATGAGGCGGATTGGTTTGACTCTAAGACGATTTTGGATGCGATGAAGATCCGAAAGGAAGCCATCGCAAAGGGAATTATTCCAGACGGAGGAAAGAATGGTTGGAGTATGAGCTGAGCCTTGAAGCTGATGAAGGATCTCGGCTACATTCAAGGCTACTACTTCTGTAATACTGCTGCAGACGTCAGACTAGCCCTCAGTAAAAAGCATATGTGCTACACTGGAGCAAGATATATCCGCTGGCATCAGACTGGAGTGAGTCATATGCTAACTCCAGACAAGGACGCAAATGCTGGACACTTGTTCGCCTTAACGGGGATTGATAACGAAAAATATCATCATCCAAACAGCTGGACTGAGTGATGGGGTGATAAAGGATTTTTTTACACCCCTTTTGGTCTCCAAAAGTGGCTGTATTCTATCGTAGCTATTATTGATAAAAAGAACGCTAGCCCTGAGGTGGTGATTGATTCAGCTGATAGCGAAAAAATGGTTCAGATGAATATCCGAAATGGTAAGTTGCCGAATGAATCATTGATCAAGCTCCACGCAGTATTGATGGTTATGAGAGCTTTCTATAATGAGTTTGATAATGACAGGGCAATTCAGAAAGCCCTACAGCTCTGAATCGTGAGGTCTGTGAATTGACCTCTGACAAAAAGGCGATTCCTCAAAATGATCTTCGTTGCGATGAACTGAAGGACAAGCAGAGAAGAGCTTATCCCAGACCTTGCAATGGAGCAAGGCATCATCAAGAACAAGGTTGGATTGGATGAGCCAGTCAAGAGGTATCACGCTAGTTTGATGATTGCAAGAGGTTTGAGATTTAGTGGTAGAATTGAGTAAAGCTAGGATGTGTTTACATACCACGCCCAGTCTTAGGGCTGGGTTTTATTCTTTGTAAATAGCAATAATGGATTTCGAAAAAGCAAAAAAAGCATATGATAGCATGAGTTGGCAACAACAGAAGCAATTCGCTGAACAGAATAAGAATAATGCTGAATTTCAGCAATTCGCTCAGCAATATCTCGGAGGGAACAAACAGACTCCTCAAGCAACTCAACCGAACAATCCTGCACCCGTAGAAGACAATCAACAAAATCAGGATACTCAAGACGGTCAGAACTGGGGAAACAATGGACGGATCCAGTCTTCTCAAAATCAAGAGACACAGGCTCAAGGAATACCACAGCAACCAGCTCCACAAGATTCGCAAAACTATCAATTCTGATATTGAACTACGGAAGAGTATAGGAAACAGAGGGATACCCAGTTTGCACAACAGCTTTATCAGTCAAACCCTAACACCTTTGACTATAATGCAGTTTTTAATTATGTGAAATCTCAAGCCCCACAGGTATCGCAGGGGGAAATCTCTAATACCGTGAAGAATATCCAAAGGCAATGGCTTCAGCATTCAAGGATTGGGATGATGGGAACGGCTAGCGTTGATCAGATCAGAAATGGAATCCTGAATGGTCAATTTAGTCAGTCTGATTTAGAACTTTTAAGAACTCAAAATCCTCAGAAATATCAAGAATATCTCGCTCATGATGAGAAGTTGAATGCAATGTCTAACGCAAAGCACAATGCCAACCTCACGAATAAAATGTATAGGTGAGAAAGTGTAAAAGAAGAGAGAAGTAATCAAGACTTGGTCATGGAGGCTATGGAAAAGTTTTTTGGGAATATCAACTATAACAGCAAGGAAATTGTAGAATCCTATAAACAAGCTCTAAACTCCCCAGAAATCCAAAGACGGAACTCAAAAGTGCTAACCGCCCAAAGGGAGATGGATGAGATTGATGAAGATTTAGATACCATGAGGGAAGACCTCACAAGACAATATCCGTGAATCTCAAAAACACAGCTCAATCAGATTCTCTATGATAGAAGCTATAAAGCAATGAGAAGGAAAAATGAGCTCCATAGGGACTATATTATGGCAAGAGGAGAAACTCAATTTTTAATGAATTTGGCTGAGAAGAACTTTGAGGCTGACATGAAGTGAGTTGAACTCCAAAGAGCAGAGTTTACAGATAAATATAATGCTCTCGGATTTGCAAAAGGAATCCTAGAATTTGAGACTCCACAGCAAAAAAGACAAGCCGAGCTTGATCAGATTAGAAAAAAAGCTCAACTTGAGAGTGAGCTGAATGATATAAACTCTAAAGACCCTAAAATTCAATTCAATGCTCTTATGAAAGCACTTGAACCTCATTATAAAGAATTTGGCTCTATCATTCTCAGACCACAAGCTCAGGTAGCTCAAGACATCCTCGCTCTCGCAAAGAAAGAAGGAATCAGTGTAGGGGAAGCAATGAGGAAGAACTTTACCGAACCGTTATATAAAAAGCCTGAGTATAGGGCGATGATGAATAAAACATTGGGGATACAACCATGACAAAAATTGCTCAACATTTGAGGAAAGCCGTATATCCAAACGACTAATGCCGATGGAACTATTACTATTTCTCAATATGAGTCTGGAAATGCTACCGGCAACATCACGGGTTATCAATGACCAGATTATACTCCTGTAAGTGCAGAAAAACTCCAAAAGGGGCTCGCAAACATAAAATTTCAATGAGATGGAAGTGTTGGAGGTCAGTGCGGATCGTTTGTAAATGACTACCTTCAAAATTTAGGAATTGGAAGGATTTTTTCCGATCCTATCCATGCTAAAAAATCAGCAGTGAACAGTAGCACACCTTCTCTAGGGAGTGTGGCAGTTATTGACTGGACGAATAACCCTAATGTTAGTGAAGCTCAGAGAAAATATGGGCATGTTGGTGTGGTAATTGGAATCAATAAGGATGGAAGTGTTATTTTAAAGCAATCCAATAAAAATGGAGAAGAAAAAGTCTTTACTTCAACTTATAGAGCTGATCAGATTTATGGCTATTTTGATCCTACAATAAGTAAGGAACAAGCCACAAGCGGGAATGGCTGAACTCTTACAAGTTCCGATATCATAAGCTATAACGATAAAACCTTGAACAGAAAATTATCAGCACAAGATAAGAATAGAATAGGGAAGGAAATCAATCGTATAATGTCGGATCCTAATGCTGATATTAATTCCATACTTGAGTATTCTGCAGGAGGGAAGGTATTGACAGACTCTTCAACAACTCCATTAATGAAATATCAAGATGCTCTTAATGGGATCGGAACACTTAGTGGACTCTTAAAACAAGCAAATACTGGTCCGATAGTATGAATCCTGAGATCAAATAATCCTTATGATCAAAAGGCTAGAGAAATTCAAACAGTTATTAATCAACTTGTTCCAACGTTAGCAAGAGGGGTTTACTGAGAGGTTTGAGTGCTAACAGATGCAGACATTAAGCACTATTCTCAAACTGTTCCAAACTTACAATCTACAGAAGAGGTAAATAATGCTATTCTTGCTCTCACTTTAAATATGTTGGCACAAGGATACAAAAATAAACTGAGAACCCTAGCTTCTGCTGGTAATGATGTGTCTAGATTTTGAGGAATTTATAATGACATTATGTCTGAAGTAGATAGTTTATTATGAAAAGTAGAGAATACTGTTTCAAAATCGGAACAGTATTGGGGGAAAGATAATGTGATAAAAACTTCTCAAGGAAATTTTAGGGATGATTTTACTTACTAAATACGGAAAAAGATGAATGGATACTGACCTAGAACAATTGGTGCGTTCCCTTGATTAACCGATGATTTAATCAACCAAATTCACAAAAAAACAGAGCACCTTTCTGGAGCTATGAAGTCGCAAGCTGAACAAGAAATCTATCGTTATCTTTTGCCTAAAGTAGTGGCAAAGAAACGAAATGAGGACAGGAAGCAGGCAAAAAATGAGTATTATAGCAAACTTATAAATTCAGACAATTCAGAAGAAAGACAGCTTTGAGAAATCAAGTTGAGACAAGAAGACCTTGCAGATATGGTTAAGGAGCAGTTTTGATTGAGATTTAATCTCTCAACAGATGAAACGATGTCGGGTTTTCAGAAATATATGGAATGAGAAGGTGTTGATCAAAAGCTCTTGACTGATTATCTTAATGGGAATAATGAAGAGCTTTTGTATAAACTTTGATTTAAGAAGAAAACATTTAATACTTGGCGGGATGAGCAGAAGGATGGGGCTTTTTGACCTCTCGTAAGAGGAGTAAGCGGTTTACGAGGAGGCGCAAAAGGAGCAGTTAGTGATACAGCAGAACATCTCTCAGAAATCCCTTGAAGAATATGAAAAGTAGCTTCAGATGATGAAAAAAACTTTATAGAAAAGTTTAATCAGATTCTTTTTTGAGAAATCGTCGCAAACTGAGTGGGTGGAACGGCTGGAAGCTTTATAGGGTGATGACTTAGTGGGCTGATAAAATGATTTACCACTGAAAGAGAAAAGAAATCAGTTAGTAAAAACATTCAAAACATGGTGAGAAAAGTCGCTAAAAGTGAGAATGGTCAAGCATTAATTCAAGCATATAATGAATTACCTAAAGAAGATAGAGAAGAGTTCAGTGATCTTTTAACCTATGCTAATGGATTGATGGATTTAGCTGGGATGAAAGCATTCAAACAGCCCGCCGAACAGGGTGTAAAAGCTCTTGCTACAGAAGGATTAAGCATACTCGGAGAAGTAGATAATTTAGCACAAGCAAGTGCAAAATCAGTAGCAAAAACCCTTGCCGAAAAATCAGCCCAAAAAAGTGTAGAATCCCTAGATAAAAATACGGCAAAAGCTTTAGAAACTGTAGGAAGAATCGTGCAAGGAGATGAAGAAGCAAAAAATATTGCCCTTGAGGTTTTTAAGAACCTAGACACTAAAGGGGTAAAAACTTATAAAGAACTTGCTGAAAGGTTGGGGGCAAGAGGAAAAGAACTCATGCAACAGGTTGATGATGAGCTCCTCAAAGATACAAAAGGCTACAATTTCTGGGATAATCTCGTAAAGAAAGAAGTTGAAACCGCAGGAGGAACAAAGAAGATAATAGAAACTCCGATCTCTGATGGGATTGAACAGCTCAAAGAACTTTATGGAAAAATAGGGGATCAAATCAGTTTTGCAAAAATGGAAGGGCTGGAAGATAAGTTTGCGAAAGAAGGGCTAAGTTTAAAAGAGATGAATGATCTTGCAAGGCAATACTCAAGCGAATTTTGAAAAAAAGCTTTTAATAAAATGGGTGATCCAAAAACTTCAGTTTCCTCAGAACTCTATGAGTCACAAAGAAAGGCTATTAAAGAGTTTGTAAGAGAAAAAATGCCAGATGATGTAGTAAAGAACCTTGATAAACAATACAGTGAACTTTCTAATACTCGTGCTTGGGTTGAGAAAATGGCTGAGAAAGTTAATACTCTTGCACAAAAAACCAAGAAAAAAGGATTCCTAGAGAAAGCATGAGGACTTGTAGCCAATGTGATAGACAAAGCCTCAGGATGAAGTTTAAAAGGGTTTATCGGAAAACTGTTGCCGAGTAATGTAGGGAATAAGATAAATAACAGTCTTGATATGGAAGCGGAATTGCCAAAATTACTTAAGGAAATGCAGGGATTGGTTGGAGAGATTAAAAAAGGAGAATCAACATTAAATTTAAAGCCTGGGGAAAGAGCTTCTTGAGAAACAATCGAACAAAGCATTCTATGAAATCGTCCTATGTATGAATCGGCAAAAAAGGAGTTAGATGGTTTTCTTGATGATATAGCTAAAAATTATTGATGAAATGCGGTAAAGCCATCATTAAAGTTTTTGAACCCTGATTGATCAATAAGAAAAGAGTGAATAGCGAGGATTATTGAAAAAGCGTCAAAAAAAGCCTGAGGAATTAGTGAGGTGAATGATATTGCCAGAGGAACTGTGGTCTTTAAAGATCAATCTTGACTCGAACAATTAGTTAATGGCTTACAACAAAAAGGAATAAAATTGGATAACAAATTTAATAATCCTACGAAATTAGGCTACAAAGATGTTTCTTTCCTCTATGAGACAAAAAAAGGGGTAAAAGCAGAAGTGCAGGCGAACGTCCCAGAAATGATAGTTGCTAAAGAATGAAAAAATGCTGTAAAATATGGAATGCTAGATCAAGCAACCTATGACGCAATCGTAAAAAAGACTTGAGTAAAAGGAGGGCTTGGGCATAAATATTATGAACAACGAAGAACTCTTGATGAACAGTTAAAAAATGTTCCTAAAAGTCAGCAAGAGGGAATAGAGCGTCAAATGCGTCAAATAGAGATAAAAAGTCAGGAATACTATGCAAAATTTAAATAAATCTTGCTTTATTTAAAATAATCGCTATAATTTGTAGTGGATATCTTTATATTTTAATGCACTTTTATGAAATACCGTAAAGATTCAGAAAACGCTGTAAAATTTGATTATCCTTTAACAAAAGAGTCAAGAGGTGATATAGAACTCTTTGACCGTGAAGCGGGAGTATTTGTTGAGATAGCAAAGCTTACCAATGATCAATTAGCAGAAATTATTTCATATATTTGAGCAAATGGAAATGAAATATCGGAAGAAGAATATAAACAACTTATTTCTGAAGAATTAAAAAAAGAAAGCTGAGAATAATACTCAGCTTTTTTATAAATTATAATTAATGTATAGTTTTATTTGCCTATAATACCTTTCAAGAAAATTAGTATAAAATTCTGTTCATCAAAAATATTTATTCCCATAATAAACAAATATAGGAGCCTTTTTAAACAAGAATCTTTCTAGCTTGTCTTTTTGCATGAAATGACATTCTGTAAGGTTGTCTGATATATCTGCTAATTTAATTGCTCGTGCATCAATATTGTCTGCACTTATTAGTCATTTTATCATATCTTCTCGTCTTCAGAATTTATCTGGATTGTTTGCATCATGGGATGATAGACACACAAGTTTCACAATATTTTCTGGATATCATAAACTTAAAAGGGCTTCTTTTGTAAATCAAGCATCTTCTATCATATCATGAAGTAGTCCAGCTAATTGAACTTCTGAAGAATATCAATATTCTTTAAGTAAATTATAGACTCTTATTGAATGCATATAAGTCGGAGTATTCAAACCTTTTCTAGTTCATTTTATTTTATCCTTCACTAATTCAATAGTTCTCTTTAGCATTTTCTTTTAGATAATGATCTAAATCAGATTTTTTAATATATCTAATTGAGTATCCTTTTTTAGACCTCTTGGAGGGTATTTGTATTGGGATATATTTTCTACCATTATTATAAATGGTAGGTTTCGTCTTCTTTTCTAATATGGCAAGTTCTACCGCTTTATATGTTTTTATTATAGGTTTTACCATACGCACTACTACAAGATAAATACTAGCAACCATATACAACATACCTAGAAACAATATCTCAAATTTCTTTATTATCATTAGATACATCATCAGAATAATTTTCTCATTTATTATTAGTCAAATTATCCATTTTTCGTCTTACTATTGAAGCAATAGACCTTATTTTATCATAATCATCAAGTTCATCAAGTCATGATAACAAGCTTTCTTTTGTATAACCTGCATGCCCTATAAATATTTCAGTCTGTTCAGGGTCATTTTCTCTATTACATATTATATATTTTGCTGTCATTCAATAAGGTTTGTCATTTATGTGATGATATATGTCTTCCATTGAATCAAAATATCCATCTTTATATTGAATTGCAATTTTATTAACTGCTCTTGTTAATGGTCATCCCTTCCAGTAGATTCTAACTGAATCACCTCATGAAAATGAATCATATTTGCATGAGAATTTTACTCATGGGAATACCTTTTTAAGGTCCTTTTTAATAAGCATAGCGGGAGTTTGTCTTTTTGATTTCATCTTGGCGGATGGTTAATAATTAAATGTTGTTCATCTATCGAACAGTCAGAGTATACTGTTTTTTTTATAAATTGCAAGCCTTTTTTACTTTTTGCTTGCAAAAAATAAAAAATAATAAAAATAAAGAAATAAAAAAACACAGCCCATCCGCCAAGAAAAAGCTGTGTATTTTGAATAAATAATAATATAGGATCCAAAATCAAGAGAAAGTAAATATACTTCCTTTTCTGAAAAAGAAAAAAAGAACGGCTGTGCCCAGAGTCCTTCTTTTCTTATTGTGATTTATCTTAGTTTAATTAAGCGAAAGAGAATTCCTCTACTCTTTCTTTAATGCTGGAAACTTTTTTTTGAGTCTCAGCATCATTTTGCAGATTTGTGACATCATACAAGTTTTGTAATCCAATCCAAAGATCAGGAGAGGTTCACAAAGCTGCACCAATCATAACTGCCAATGATGGTGTGATGTTCTTCCTTCCCTTGATAATATCATTAAGGAAAGGTCTACTAATTCAAATGATCCCAGCAAATTTAGCCTGAGTCCAATTCCTAGCTTCTAGTTCCTCTTCAATGTATTCCCCTGGGTGAAATGCTACAGTTGGATTAAACATCTTTTGAAATAGTAAATAAATAAAAATTGTTTACTCTTAATGGCTTGCTGAGCAAGTAGCACTACTATTCGTAGTGCTTACTTAGCTCAATAATTCAAATAACTTGAATATATCCATCATCCATTATCTTGAATATCAACCTCCGTTGATCGTTCAATCTAATGCTCATTTGACCCGTACGAGATCATTTGAGTTTTTCCAAGTTGAAAGATTTCTGTTCCCTTAGGTCCATCAAATCCTTGATTTTGTAAAGGTAGTTCACTTTCTTTTGGAAGGTCCTGATCAAGTCTGAATGATAATTTGATCTCTTCCTTCAATGATAGACATCCTCAAGACTTGAATCGAGAAATACAATTTGCATTTCCATTCAAGAATTAAGAAATAAAGTGATCAATCTAATGAGTTTTCGTTTTCTTCTACCTCGTTAGATTGTTATCTATTTTAGTGAATTTTTTTTAAATTGCAAGAGAAAGTTATACTTTTTTGTGAATAAATGATTCTATTTTTTTAAATAACTGAAAAAGAGCTATTAAGAAGCCCTTTAACAGAAAACGAAAGATAGATTGACCACTACAAACACGACCAAGATTACGAGCAGAAGCGTTTGTTGAGAACAAGTATAAACATTTTCTTAGGAAATGCAAGGAAAATGATCTTTTTTAGACCTGTCAATATATTGTGTATAATGTAAACAATAAACTACTAGATATACTTTTCTAGCTTTCCATTCTCACAAAATTCTCGATTTTTTGTGAGTATTGAGAATGAGATCAGATTTTTCTACTCCAAATAATACTCCTTCCTCACCCTAAACTTACCATTACCCAGAATCATCAGTTTACTAAAGACTTCCTCCGTTATATATGCAGGAATTTCCTCTGTAAACTCACACCCTATCACTACACATTGATGAGCTATAAAGTGGGTTAACTCGTGAATGAGAGTATTTAGACTGTAGCTCCTGAGTCGAATGATATTCGTGTATTTAAGTTCTTTGGAATAGAATCAGCCTAGGTCGTATTCAACCTCGCTAAGTCCTAACTCTTCAAAAAATGCCTTATGTCTCGACTCTCCTATATCTCAAACAAAGACCTGGAGAAACTGTCTAAAGGTCTCTGTTCTGAGTCTGTATTCTGTGAGTCCTACCGGGTGGTGGACTATCTCAGTATGAGGGATCAGATTTTTAGATTTTTTCATTTTCTCAAAAGTAAATAAAAATCAGAATTAATCTTCTATTTTCTCCATCCCTCGAGGATGGACTATTCTCCCCCTATGCTCAGATTTCTCTCAGTCTGCTAAGTATGCAGTCCTTGTGAGTTGTCTCTTTATGGTATAGGTATCCTGAAAGGGATAAGGTATCCCAACTACAAGCTCTCGCTGATCGAGGTAGTCTTGAGTATATCTTACTTTGTCTCAGGGGTTGAAATCTGATTTCATTCCACTTTTATATTATCAATTAAAAACTTCCCTAGCTCCTCCTCTGGAATCAGTGCTGATTCTAAGAGTCGGTATTCAGGTTCAAAACTAAGAGCAAGAACCTCAGACTTAAACCCAAGAGCAGACATTAATGGCTCTTTAGCTCGAGATTTTCATATACTTTTTAGTAAACTTCTATCACAAACAAACTGCCAAAGTCCACTCTCTAGGCTTACTAGTTCACGAAATGTTTTGAACTTTTGGTCGTAATGTTCCTCTTCTGGATTGCTATAATCCATATATCAAATATAGATATTTTTACTATTCATATCTAAAGTTAACAATTCAATATCATCTCAGTCTATTCCCCAAGGTTTCCAACCTTCCTCTATAAGAGAGGTAAGTAAGGTTTCTAATTCTTGCATTTTCTTTTTTTAATTAAAGATAAAGGTCCAGTGTAAAGAGTTTTTGCAAGGATACCCTATTTTGAGGCATCCTTTTTTTGTAATTCATTCATTTTCTCAAGAGCATATTCCAGCATCTCTTCAAAGTATGTTCATCAGTTATAATACCTTAGATGATAGTCTAGAGTTCAATCTGTATTTTTTGTCATAATCGCAATATCACCATCATCATCAATATCACTACCATCAACTGAGTATAGCACTCAATTCAATACAACATACTTCCTACTATGCTCATCAAGGAATTTTTCAGTATAATTTTCATAATAACTCTCCAAGTTTCATCATACCTTTTCTTTGCACCATTCTTCTATGCTTAAAGGAGAAGTTTGTATCGGAGTAAGTTTCCCTTTTTTGTGTTCTATTTGACTCATTGTTGTAATAAAAAAGAAATAAAAGCCTGATTTATGATTTTTTATATCTAACAATTTGAAACTCCAATTCTCAATTGCACTTGATATCTAACGAATACTCATCTCAATCAAGTAAAAAGTTTATACAATCAGCTAATAATCATAAAACCTCTTGTCTGGTTAGAGTTTCTTTATCCAAGAACTCAGTTCATTTTTTATTGAGTTTTCAAATATGAATCCCTGCGATTCATCTACATACTCAGTATTTTTTCATCTATTGTTGAATTAAAGAATAAACATAGTCTATACACTCATCTGATTGCTCATCAATACTCAACCCTAGCTCTCTCCAAAGTCAAGGGCGACTCAATTGACCAAGAAGCTTGACAAGATATTCTCAATAGCTTATCTCCCTATTTGCTTTTATTCGTTTTATGACATCTCATATCATTACAGGGTGTCAGATAATCTCTGGGGTATATCACAAAGAAACCGTAAACTCTTCATTTTCTCTGATGGATAGATATGATCAGGTTTTATTCTGCCTGATAAATATCACATCTTTTCCATCATTTATTCTACATCTACAACCAAATGTTCTTTTCTTGTTTGCCATCTCTTTATAGATGGCAGTAATTTTTTCTTCTCTTGTCATTTGTAAAAAAATAATAAATAAATCTGACTCAATGATTTCGTGACGTCACGAATACATTGATAATCCGGAATTTCCGGATTTTTGTCTCCGACATCAATGTCGGAAACATATGGGATCTACTAGATAAAAACTCATAAGTGGCGTTGGTAACGACATCGATGTCGTCACCCTTTTCTAATTACCCCGAAATCGGGGTAATTAACTAAGCATTGACTAGCTCAGGGTTCTCGTAGATATTGCCCACCACCTCATAGTGAGCATACGAATACATCGGCTCATTGCTCTCTCAATTCATGATAAAATACTCTAGATATGACTCATCTCGATATACTCCCCAAAACTGATCTTCTATCTTTACTATATCGCCCTCGTATATCTCCTTACCGTTCTTGTCTAGGAGTCAGGTGAATTGCATGGGAATATAATCTCTATGTCCCCATTCGAGAATTAAAGACAGCATAACAGTTTTGAGATTTCTAAGAATTCCCCAGGATATTATTTTTTTGCCTGTCCAAGCTCTGAATTTTATTGGTCTCATCTCTACTTTAGTATAGAAACTAAAAAGTTTATTGGATCATCTTCCATTGAGAGCGTAGCTACGATTATGTCTGCATCAGAATAAAATCCTCATCATCCAGCATACAATAAAGGGAAATCTGACCTATCTCTCGCTCTATCGAATTCCACTTTGTTCTGATGCATCAACCATTGAATGAATCAGAATTTTTTACTAATGATTTGCAAAATTGCCTCATCTAAAGAACTATGTTGAACTATCGGATTTCCCTTTTTACTTTTTCTAGTGCAAAAAAATCAATCTTCATCTATTATTCATAACCATTCTCTATCAGATCAAAGTGATCTTTCATACTCATTGAGGAGTTGTAGTAATTTTTCCATAGCGTAATAATAAATTAAATAAAACTGATTTTTTATGTGGGTTGTTCTAATATCTACAATTTTTACTTCTTTTTTGTGTCATAAAATCTCCAAAGGGAGAGCTTTAGGCTCTCACGGCTTATCCTTCCTCTGTATAGAGCCTAGCTTAGCTAGGCAAAATATGTTAAGCTACCTACAATAGTAGGAATCTATAATTAAGCGGAGGGCGAGGATTTGCACCTCGCAGTGAACAGCCGAAATCTCAGACAAATCTTCCACATAACGCTCTCAGCATCATAAACCATACTGCCTAATTACGTACTTAAACAATATGATCTGTTCTGCCTCCTGCATTACTATTCTATTCTCTCCTCTAGCGTCTACCTATTCCGCCACCTCTGCATATCTCCTCATCAGAGGAGAAAGCTGGCTATAAATACCAATTGTTTTTATCCCATATAGCCTTGTATTTTTCAAGAACCTTTATCGCATTATTGACTTCGTCAATATCTTCTTGATTCTTTAAAATGTTTTCGGGTTTTAAATACCCTCAATCAAAAAGATCATATCCCCAATCACTAGTGTATGGAATATCATCCTCATGAATCTCTTTTGTCTCAAATGTTTTCATCTCGATACAAAATAATAAATAAAAATAAAGTTTAATACTTCGCCCATCAAGGGCATCTTACCTAGCAGAACTTCACAAACTGCTAGGTGAGAAGCCCCTGATAGGAGGGCTTATAACAGCTTATTTTGAATATTGGTAAGGATTTTTTCTTTTGCAAGGTTGTAGAAGTTCTTTTTAATCTCAAATCCAAAGGATTTTCTATTGCACCTCGCAGCTGCAAGAAGCGTTGTTCCGCTTCCTGCACACGGATCAATAACTACTTCCTCTTTATCTGTGAAGATTTCTATCAACTGCTCTAAAAGCTGGACAGGCTTTTGGGTTGGATGAACTTTTGCGGTTTGGGTGTCTTTCTCCCACTCAAAACAATTAAATATCATCTTTCCATGGTTATTGAACTTCGGCAATTTATCTCTGTAAAGGATCAATCCGTATTCACAGTTTCCGACCACTTTCATATTTGCTTTCAGCACTTGGGCTGAAAAGTTTTTGCGGAACACAAGGTTGATATAGTTCTTGAATCAGTATTGAGGGGCTTTTTGTATAAGCTCAAATTGCTGTCCATACTCACAAAAGACTACGATACACGGAGCTTTCCCTGCTTCTTTAGGCTCAGGTTTCAGCATTTGATTCACATAATGCAAGAACTCTGAGATTTTGAAGTTCTTATCAGTATCAAAGAACTGTTTCCCTGCGAGTTCTGATTCTCAATTTGCATTATCTCCTCCTTCATACCAAGCAGGATTACTCGCATAAGCATTATTCCCTAGATTATAGGGGATGTCGGCTATAATCAACTGTGCTTTCGGTATTCAATATTTTTTGTAGTTTTGGAAATGGTCATTGTAAAGTTTTGGTTCGTAAAGCATTTTCATATTTTTTACTCATCTGAAATACTAGCGTTCATCTTTGCAATAATATCTTTAACATTCGCTTTCTCTCCTTTGACTATTCAATCTACTTCCTCGGTAATAACATCAAAATCCCACTTAATTTCAGGTTTTACATATTGTTCTTTGCCGATTCTTTGATTGTAGTCGTTGTTAATATCACTAATCGCAGGAGTAAAACAGTCTTCAATCTCTTTTGAATAAGGATTATCTGCGAGGAATTTATCTTTTATCTCTAGCACTTTCCAAGATTTTGAGCTTCATTCAAGATAGAACGGATAGTTCTCAAGACTAAAGACCATCTCTGCGATTTTCTCTTTATTCTGCACGAAATAGAGAGCTGTTTCTTTGGTAATATCTACTTTGAAAGCTGTTGTCTCTCAGAGATTTTTAACCTCAATGACATAGACTGAAAAGATGGTAGGAGCCGGAAGATTAAGAATCCTTCTCTTTTCTTTTTCTATAGCTTCCTTAATCTCTACCTCCATTTTCTTTCCTGCTTCCTCAGTGAAAAGTCCTTTACTGATTTGCTCTGCATAGTTAGAACGGACAATACTTTCAATTTTGGATTTATCTAGTTTAGGTCTCTTCAAAGTTATAAAGTGTTCTGGAGAAATGGTATTGACCGCATCTGATACAAACTCTAAAGTAAATTTGCTTGTGTCTTGCGTTAATTTTCTAAAGTGTAGTTTCATTTCAAAAAAACAAAATATAAAATCTAAATCTTATCTCTGAATATCCGCAATAATCGAATAGAGAGAATAAAGAATAGGAGGGTTATCATTCAGTGCATGACGGGAATTACATAATAAAATCCTTTTTAAGGTGCATTTTAACGATATTAACATACTCACCGATAACGGTTGTTCTGTTCTGGAGGAGTTCAATCTTCGTTTTTAAAACGGAAAGATCCAAATCCTGTTGATAGAACTCTTGCCTAATCACAGCCTCTGCAGTGCTTTCAGTGTATTTTTTCTTTCAGTTTTCATCGAGCTCGGCTTTCAGCTCAATCATTCTTTTCCCTTTCTCTACTTCATTTGCCTGTTTCTGTTCTTGGTATTGGCTTCTGAGTTCAGTAATACTATCAGCCATCTCTTCCCAGAGAGCTACTCGGTCAAAAAGGTCTTGTTCTCCGAGTTGGAGTCCTTTTAATTTTAGCACTTCGTGTCTCTGCAAGAGGCTATCAATTTTTGTTGTTTCCATTTTTAATAGTTTTTAAAGTAAAATTTTTCTGTTTCCAAAGGGGAGGAGGAGCTTTCTTTCTCAGCTCCTCGCATAATCTTTCTGCTCTTTGGAAACGATTAAAAGGGAGCAAAAGGGTCTTCCCCGTCGTATAAAGCCTCTAATCTCACCCCATTTGCTTCTTCTAAAGCTTTTGCGTCCGTAAACTCGGCTTTACCTAATGCCATAATGGTGTAGGTTGTTTCTAATTTTTGTCCTGATCTTGTAATTTTTAAATCATATTCCTTTGGGTCTCCGAAGTCAGGGTCTCTGGATAAAGCAAGGAGTTGTTCCTTAAGAGATTTTTGGGTAATGCTCATCACCTGAACCCTTTTTTCATCGTAGTTATACACTACGAAAGCCCAAAACTCCTTAGCCTTTCTTCCATCTTTGGAGTCTGCTGGAATTGCACTATAAGCAATTCTGCTTTTGACTGGTTTATTCTCGTTAGTAAAGTATTCCCAACCAATAATTGGAGAGGTTAGGATTCTGATTTTTGTTGTTCCATCTTGGAGTTTGGTATACTTCCCTCCTTCAGATTTTGGAAGTTCATAGTTGTTTGGTAATCGTGTCATCTGTATAAATTCATAAAAGAATAAAAAAGGTTGGTTGATAAGTATTATTTTTTACTTTTTGTTCGATTGAGATATTGTTTCAGTGTTATCTCCTTTTCTTTCTGTGTGAATGTTTGATATTCTGCAAATCCTTTCACTCAGAATTTATGAAGAAAAATTCTAAAATTAGACTTTTGCTCTATTTTTCTCTGCTCTTGTGGTGTCATTCGTAAAGTAATAGGAATTAAAGACTTTCTGCATATATTTCATAGGGCTCATTATTGTAAACATAGCCGAATATGTGGTATCCTCATCAGTTTCAGTATTCATCATAACCATAATCTCAGTCCTCAAATTTTGATATAATCTTATCTTCTTTAAGCTTCTCTAAGAGTCTCTGAGTATTGTTTTTTTGGATTTCTAGCTGTCTAATTCTATCTTCAAGATTTGAGTAATTGTTTTTTATTTTTCTCAGTTCCTCATTCTCTGCTCTGAGTTGGTTTACTTCTTCAATGAGTTTCCCCTGTTGCATGGTTAGGAGTTGCATAACTTCCGCCCGGATTTTCTCCTTTTGCTTTCTTATCTCTTCCATTAGAGTTTATTAGAGGTTAAAAATGCTGTTCCTGTGAATCTGGGTATTTTTTCTAAGAACTCTTCAAAAAGTTCTGGTGTCTTGATCTTACTAATCAGGATTCCTCCAGCAAAGCCGTTCCCTATTTTTGTTCCGTAGGAATAAGGGATTGCAATATGGAGAATTTCATCAAGCTCTCAAGTTCTAGGATGTCTCAAGGTCTTCCTTCCGATGATTTTGTACTTTACATCTTTCCACTCTGACTCTGAGATTTTATGTTCCTCAATGAAAGTTGATTTATTATTCTTCATCTCTCAAAGGATTCTGTTTTTCTCCTTTTCTGCTGTAATCTTTCTCCTAGGCTCAAAAAACATTTGGATAAACTGCTCATGAAGTTCTGTCTTTGGTAAGGTAAAGAATCTCATAATCTTTTTACCATAGCCTTTAAGTTCTCCTTTGATCTCTTGAAATCATTCATACCCAACTGCAATCATATCCTCTATAAACTTCCTCAATTCTGCTTGTTCTTCCTCGGAAATTGTAGGTTTAAGCTTTGATAAGTCTTGCATAAGTCTAGTATAAATCATTTAAATCAGGTTTTTTACCTGAGTCTCAGGTGTCATAATCATCATCCCATCTCCCATCATTAAGCCACCCTTGAGCTCGCTTGATATAGCATTTCTCTGTCTTTTTATGCTTAAGTTCTAAAGTGTATTCTCAGGCTTTTTTGATAATCAGATTTGGGTCATTCCCTCACTTGATAGCACTAGTCCAAGCTTGGAAAGCTTTTTGTTTCCCCTTTTTTAAGGGATAAGCTTTCCAAAATTCTTCAAACTTAGGAGAATATCAGATTTTTTTTGTTTTATTTATTTTATTTTTTTTATTTTTATTTACTTCTTCGTTAGAAGAAGTTATTAAATCATTATCACTATCACTATCATTATCACTATCACTATCATTATCACTATCATTATCGGCTTTTTTGGGTTTCTCTGGGTTTTCAGATAACCCACTGGGTTTTTGTGAGTTTCAAAAGTTCTTACTTGGTCTTCAACCACTTTTTCAATTCTTCTGATTTCTCTGACAGACAACTTTATATTGTTCGTCATCTTTAACCCAGTCTTGAGTCATTACCGATAGCAACATTGATACAAGTGGGTCAATTCACTCTGGAGGACTTCAATTGGTCTGATAGTGAAAGATAGCATCCAGCAATTGTGCTTTTTGCTCAGTATTCAAGAGTTTTATTTGCTCCTGATACTTCTTCCTGATAATAAAACTTTCCTTCATTTCCTATCTCTCAATAGGAAATAAAATTAAGTTCTTTTTTTGTTTCTGTTTACTCATAGCCAAAAACCAGACAGCAAAGCCAATCCCTGCTCATAAGCAAACCGTAGGGTTGTGGATGAATCGTGCACCCATTCATACCACACCTAAAAATTTGATTACTAAGAAAATTATGCTCCTAGTCATTGGTTTTCGGCGACAAAGAAATAAAATCTGACTGCTTCATACAATCAGATTTCTTTTTTTCTGCCTATCTCCTTTATCTTAGGAGGCTGATTTCTCAGACTTAGCAATAGAAATAATAAAAATCTGAATTATATGACAGCATTGTTTTCGAGGGGCTTCACTTCCTCTAATCACAACACCGTGATATAATTCAGATAGTGTTGTGTGAAGCCTGTTTGTTAATTCTCACAATAGGCTTGCAACACTCAGTATTGAACATACGAAAAAGCCTATTCCTTAGTAGGAACAGGCTATAAGTTACTTCTTCAAATTGCCCCGACCCGGAGTCGAACCGGGGTTACAATCTTCGCAGGATTGTGTCCTATCCACTGAACGACCGGAGCAACGAGTCTGAATATAGTGATGTACTTAAAAAAAGCAAGATGTCTTTTGATTCGATAAGAATTGGATTCTGAAAGGAGATTCGCTTTCATTGCTTTTGGGAGATGAATAACTATGATTTGTGCATTTACCACCTTTCTCACAGAAAAAATATGTTTATCTTGGAAATAAAGCTCGATCTCAAGAAAGACCTACAAAATTGGGTGGATGGATGCAATAAAATAAGTCATGGCAAAAATTGGAAACTTGGAGTTTCTCCTGAATATCAACGTATTGTAGAGCAACTCGTTGGGAGCGATTTTGAAGAGGCTGAGAAATTTATGTATCCAGTTCTTGAAGGTATCTATGAAGAGAAGAAAAGTCTGATAACAAACTATAAAAACATCATACAAGAAAAGATCAATGTCGATCTACAAGAAGCCTGTCTCGCTATGGAGGATATGACAGGTTTTCCGCTCTATAGAAAAGATTTTATTCTGAATTTAACGACCTTTCCTCGTTGACCTTATAATAAAGAAACTGGAGAATTTTGGAGCTGTGTTTTCCGGTCTCCTGAGAATATACTTTGAAATTTTCTCCATGAATTACAGCATTTTCAAGTTCTCCATTATTTTAAAGATACTCCTCCAATGAGTAGGCTTACCCGGGAACAATTTGAATTTCTCAAAGAGTCGCTTACGGTAATTCTCAATGTAGAATGTAAAAAGTTTTTAGCACGGGAAGACAAATGATACCCTCTTCATCAAGATTTAAGAAAGAACTTATTGGTATTTTGGGCTAAAGAAAGAAATTTCAAGGCTCTTATTGCTTACTGATGTGATTGTGTCAAGTAAGTACAAGTCCTACAACTCCTCCTTAATCTTCATCAAAAGCTGCAAAGCCTGAAGCGGAGTAATCGCATTCAAATCCATCCCTGCCAATAACGCCTTTACCTTTTCATACTGGGCTTTATGAGCAAACTCTTCTTGCTGAACTGCGAAAAGTCCCTGAGGAGCATTCAAAGAAGGAAGATCAGATTTTTTTCCTTTTTCTAAAGCAGATAAAAAGCCTTTCGCTTCCTCTAAAATCGGCTTTGGAATCCCAGCAAGTTTCGCGACATCCACACCATAGCTTTTACTCGCACCTCAAGCACTGATTTTTTTCATAAAAACGACTTCCTTATCGGTTTCGTAGACACTAACTGAGAAATTTTTGATTTGCTCGCTTTCCTGTTCAAGGGCGATGAGTTCATGATAGTGAGTTGCGATCAGGGTTTTTGCCTGAATGTTTTTCAAAAGATAATGCAGAATTGCTTTGGTAAGAGCTAGTCCATCATAAGTTGAGGTTCCACGCCCCAGTTCATCAAAGATCACGAAACTCCTCTCGGTCGCATTATTGAGGATATTCGCCACCTCAATCATCTCCGTCATAAAGGTAGACTGATTTTTCGCGATAATATCGCCACTTCCCACACGAGCAAAAATTCCATCAATCAGTCAGATTTTGGCTTCTTGTGCAGGAACAAACAGCCCACAATGTGCCATCAAAACAATTAAAGCTGACTGCCTCAAATAGGTGGACTTACCTCACATATTCGGTCAAGTGATGATGTGAATCAAGCCGTGATGCGTGCTTTTGGACTCGCCAATCTCCAAGGCATTAGGGATAAAAGGCTGATCCTTCGGCAAAAAGGCTTCAATCACCAGATGGCGACCTGCTTGAATCTCAATGATCCCGTTTTCTGCGAGTTGTGGCTTGACTAGGCGGTATTCCCTAGCAAAAATCGCCTGTGAAGTAAAAAGATCCAGCCATGCCAGCTTTTCTGCTAAATTATAAAGCGGAGTGATGCTTTCCAAAAGCGACTGCTGAAGCCCTTGTAAGACTGCGGATTCTTTGACCTTGAGTTGCTCTTTGGCACCGAGGATATGAGATTGGAGATTATCAAGATAAGGACTCGTATATCTTTGGTTGCCTTTGAGGGTTTGTCTGCGGACAATCGCAAACTTTTCCTGCTCCTGAGCACTCAATTCGGTAAAATCTTGCTGTGCGAGAAATTCCTCAAAAAGCTCGCTATCTTTACTGGTCAGCTCTAGGAAATAGCCTTGATTCATCACAAATTTGAGTTTAATATTGGAGATTCAGGTTTTTGAGACTAATTCCTGTTGATATTGCATCAAAATTTCATCGCTATGATAAGCGATCTTCCTCAGCTCATCAATCTCCGCATCGTAGCCCTCTGCGACATAATCATACTCTCCCAAAAGCTCTTCATCAGCCTTTAATGCCTTTTGTAAAAGCAAAAAAATCTGATCAAGTGCCGAGATTTCCTCCTCTGTGAGTTGGATTTTTTTAAGTTCAGACAGAAGCGTCCCTTCTAGCCCTTGTAAAGCATTACGAAGCACGGCTCTCAGCTTCATAAACGGACCTCGGCTGAGCTTTCTATACAGAATCAAGCTCAAAAGTTTTGGAATATCAAAAAACTGCCCCAAAAACTGATGAATCCCAGCGGTCATCGGATCTTCCAAAAAAGCCTCAAGTTTTGAGAGTCTTCGGTCAAGTTCAGACTTGATATTGATCGGATTTGCAAGTAAATAGCGAAGAAGTCTGGAGCCACCTGCGGTTTTGGTCGTATCTAAGATGCCGAAAAGGCTGTATTTCGTGCTACTTTCATAACTAGAAGCGAAGAGTTCCAAGTTCTTAATTGTCACTTCATCCATCAAAACTAAGCCTTCCGAACTATGAAATCTGATTTTTGAAATATTGGTGAGCTGTTGCTGCTGGGTATGCTGAAGATAGGCAAAGAGGAGTGCCATCGCCTCCAATCTCCCATTCTCCAAAGCTTTCCCGAAACTGCTGGTATGTTGCACCTTACAGAGCGAAGTGATGAAATGCCCGGGATCCACCGGCACTGCATAGACGGAAATCAGACACTGCAAATACTGCTGAATCGGCGTAATCAGCTCTTCCTTGATCCTCGTATCCGTGTCAATAATAATCTCGGTCGGACGAAGCTGAAGGATAAATTTCTGCACTTTTCCCAGTTCATCAAAGCTTTTTGTCGTGTATTCTCCGAGCGTAAAATCTCACCAAGCGATATGATAGTATAAGCCTGACTTTTCATTCGTGGTCGTGATAGCCAAAAGGTAAGAAGCCTGTTTCTGCTGTTCGGTAATGGTCGTTCAAGGAGTGATGATGGATTTGATGCTTCTCTCTACGATTTTTCAAGGGATAGGATCAGTGGTTTGCTCGGCGATTGCGACTTTATAGCCCTTGGCAACCAGCCTTGGAATGTATTTTTCCACGCTATGATAAGGGATTCACGCCATCGGAATCGGATTTTCAGAATTTTTATTTTTGGAAGTAATGATGAGATCGAGGATTGAAGCACAGATTTTCGCATCCTCAAAGAAGACCTCGTAAAAATCCCCAATACGAAAAAACAGAATGCAATCAGGATTTTGCTGCTTCATCTGCACATATTGCTTCATCGCAGGAGTGAGGTTTTCAAGATTCATCAAAATAAGACTAAACCAGAGTAAATTCAGATTTTAGCTTATCGTTTCAGGCTTTTTTTTCAAGAGATAAAAAAAGAGAAAGCCCGAGGCTTCCTTCTTATTTTGTCTGATCTTAACTAGGCAAGCTACTCTAACTTCTGTCCAGTTTTTGTAAGAGGCAATCACTCGTGCTTGAGAGAGACTATTCTCAATACTTTTCAATAAACTCCTTGGCTTTTGTTAGAATGATCTCAATTTTATCACTATCTGCTAGTTCATCTTTTGTTAATGCGAGCTTCACACTGAATGGACGGAGATAACCAACTTTTCTTATCTTTTTGAAAAACTGAGGAAGTTTGAGAATCCCCTCTCAAGGAAGCAAATGTGTCGCTCCAGACTTTGATTTGTCAGAAATATAAATCAAAGCCAAATAAGGCGCAAAATCATCTAATTTATTGACAAAAATATTTTCTAGATCATCTGAATTCATATTTGCAATATCTAAAGCAAGATTACAACTGTATTTTTTGATAATATCTACGACATTATTGAAACGATACGCAGGAATTGGTAGTGCAAATACATTCGTATCTTCTGGATTGATAATTGCAAAATAGAGTGAGGGATTCTGATTTTGATATTCCGAAATATTATTTGAGATGAAGGAATAGGACTTAAAATCAAAAAACTTTGGGGCATTGATCGTAATTAAATCGCAATCCGTTGCTTCGCAAAGATCAAGGACTTTATTCATTTCCTTGCTATTGAGAAGAGAAGAGGTTTGAATTGCATAGATAGGAAGCTCATATTTTTTGACGAGATTTTTTACGTAATTCTCATTTCGAGCATCAAATCCTTTTGAAATAGCGAGATCTATACCGTCATACCCGCATCTTTTTGCCATATCAAAAATCATATCCAAGCCGTAGCCGATCATCGTATCTGTGCTGAGAATAATCTTATTTCTCATGAGTCTAATCGTTTTTATTTATTAAAGAATAACTAAGTATACTTAAAAATCTGAATTTTGTCAATTTTTAGTTGAGAATAAAGAGATTTGAAAGAAGGTATGCTGATCCGATAAGCAGAAGCCCTCAAAGTGCATAGATAATTGTCTTTTTGAGCGATCAGAAAGTCGCCGCATCATCAGGCTTAGTTAGCATTTTATAGGTCTGGAGGATGATCATAACCAGCAAAACGAGAGAAACCAGCGCTAAAGATCGGTTAATCACTGTAAAAAGAATAGGAATCTCTTTTGGGTTTAGAATCTGGGTTCCGATTCCTGAAAGATTTTGGACTCAGGTTTTTAGAACTGTTTCCTGTTTTCCATACACTGCTTCTACAATTTGCTTGGCTGCAATTATAAAGAGCATTCCAATCGTTGTCCAAGTGATCACTCCAAGTGCTTTCTTTTTGGTTCCGTCATCTTGTGCAGTAATATAGGTAAAGACGCGGATCATCATCATAATTACCAAAAATCCTAGTGAAAGATAGATAGCGATTTTTATGAATGGGAAAACGATGTTATTATAGAGCTTATTAATCCAATCAGTCGTCGTCATCGCCTCCCCTACTCCTCCAAGGAAAATATCAGAAAAAATTACCGTAGTCAGATATTTCGCACTAAACATAATAATGATTCCGATTACACCATAGAACATCATTGTGCTTCACTCCTTTACTTTTGCTGCATCACCAGAAACTAAGACTTGGTAAAGCCCGATCATCGCTATTAGGACGCCGGCAATCAAAATTACTGGGAAAAGCACATCAACGACCTTTTTGATAATTGCAGCAGCTTTGGATTTCATGAGGATACTGGTATCTGGTGTATCAAGATGCTCTCTAATACTCTCAACTCCCTGACTTAATCCTTGGGAGAAGTTTCCTCATTGAGCATTAACTGCTTGTCCTGAAAAGGCTCCTCAGAACAAAAACAATCCTCAAAGAAAGGCGAATAATCGTTTTCTGATCATTAATTTCATCGGCATCATAATGAAAGAATTAAATTCTGATTTATATTTTCTTTTTTATCCAGTAAGATAGAGGGAATATTGGGATAAGAGTTGAAGTCAGGTTCATAGTAAGCATCCTGGCTAATCGCCGGATTAGAAATAGCCCTTTCCATCGTAAGTTACTCGTCCATACCCACCGACCAAGTAGCCCCAATACTGACAAGGTGAATAAGGAATAAAAAATCTTATCTTTGGTGGATTTGGGAGCAATATAGGTATCTTGGTAAAATTCTAGCTCTTCTTTGAGATTTCAGGATTCTTGATTGGTATCTTTTTTGCTCAAGATGAGCAACGAGATTGGTATTTCTTCGGGATATCTTTTGAATAGCTGATGAAGTCTGATTTTTTGTTCTTGTGGAAGAATATCGTATCATCCGAGCAGTAAAATAACCCTCATAAAAAGCAAGAATACAGTGCATAGCATCACGAGAAATGAAGGAGAGAAAAGCGTAAAAAGAGCTATCATAAATCGCCATACTTGAGCCTCTTTGCTCCATCGATAGGGTTTCTCAAAGTGTTTGGCTGAAAACCGCTGGTATTCACTGTAAAATGGAAGGAAGGAAATCAGCATTTTTCTTTTTTGTGTAGCATCTTGCTGGATACGGGTTAGAGAGAAAGAGAAGCCTGAAACAATCGCTATCATTCACAGACATATAAGGGAAATCACAAAGTAACCTATTATTTCCGCAATAAGAAAGAAAACCTTGCTTCAAGTCCCGCTTCCAATTGCGAAGCTTCCAACAAACAAAAGCAAGAGCAACCATAGCCGATTTCCAAATTTACATCGAGAGGCAACGAAGGAATACTCATCAGGTCTCCACTGTTGTTGCTTCTTTTTTATTGAGTGCTGAACAAAAAAAGAGAAAAATAGCAAACCAGAAAGGGCCGAAAACGTTACAGGTGGAGAATAATGGAGTTTCCTATCCATTCAGGAGTAGTTGAAGTAAATAAGCGTTATCTGCTCTCTTCAAGTATACTCAATACGCATCCTTTTGTCAAAAAATCGTATAGGAAAAACTCGTGAAGAAGAGATTTCATAAAGCTCTCTCCTCAAGCATTACTACAATCGTGCTAGAAAATTATTTCAAGCCCTCCTTAAGTTTTGCGACTTCATCCGTCATCAAAAAACGAACCCCCATTTCTTTCAGTTTTTTTGCATATTCTACACTATTTACGACATAAGCGACTCCTTCTGCAGATTGATGGTTCAAATTTTCAACAAGCTTTTTGGAAAAATTTTCAGCTGGCGTCATAAAGTAGGAAAAATAGCTTCCAGAAAGCTGATCCAGTGCTGTTAAGCTAAAAGTATCTAGTGCCGAAGAAACATCTCCTCTCTTACCCAAAACATCAGCAAGTTTCTCATCATAGGAAGAAAAAATCGTATTTCCATCCATCTTATATTTTTTCACCAATGCAATAGCCTTGTCGAGAAGGAGCTGATTATTTTGTTTGCATTGAGGATTGGTAGAAGTTTTGAAGTCTAGGAAAAAGAAAGAAAACCGATTCTTTACCTGTGGAAGGAATTCTTCTAATAAAAGAATATCTTCTCCATTTTTAAGTTTACATTTTTGGAGCTCTTCTCGCGTCATCTGACAAATTTCCCTTGTTTCCTGTTTACAAGTGGTATGAGGATAAATTTTAGGACCATGATAGATCACTAATTTTCCGTCTTTAGTACTACTAACATCAAACTCAATTCCATCTGCCTGAGCATCGCGAGCAAGTTTGAATGCAGCAAGAGAATTTTCGGATGCCTTTTTTGAAACTCATCTGTGAGAAATGATATAGGTTTTGTCAGCCGTATTGGCATAAATATCTCCTTCCTTTATTCCGTGAGCTTTCATCTGGTAGGCAATTGAGAGTCTAAGGTAATTCAAAATTTCATCCACAGGGAAATAAGACGCCCCATCAATAATTTCCATACGATTTAAGTCAATATATCCGGCTTGCCCTTCGGCATTTGCGATATTTGATTTCTTTTGATCATATCGATGCTTTACACTCCGATTTCTTACTATTGTTTGAGTAAAGAGATCGTTATATTTTTGAAATACCTTTACCTCTCCACTTCAAAACTCACTTAGTAAAGAGTAAAAGAGGTCTGTTTGCTGATAGAGATGGTCGTCTAAGGTATGAGCTGAGATTCAAGATCCTATAGCAAAAGCCATAATTTTAGCAGCAGCTGTCGTTCCTCGCTTGGCAAACTCCTCGTCCTCAAGAGGTACCATTTTACGATGATCTCCTACAACCATAAGTATTCCATCTTTGAAAAAATCAGTTTTTTTGAGCTCTTGATAAAATTCTGAAAAAGTTTCATCTTCATAACGATACATTGCTTCTGCTGTGCGTCCATAGGGAGTATTATAAGGAGTATGTGAAGAAATCGTCTGTAAGGTAAGGAAGAAGGGAGATTTTTGCTCTTTTACTATTTCTAAAGCCTTGTCATATAAGTCTTTGTCAGGTGCCGCATCAAAGGTATATTTTTTCCTGTCTTCAAAAGCTTCCTCTCCGATAATGGTTTGGTAGCCGGCTCTTTTGAGGAAATTTCTCTGATCCAAAAACTCGAGGCTTACCGTGCTAAGAAAGGTCGTTGCATAGCCAAGGCGATTAAAAAACTCCGCCAAAGGTGCGACATATCCCGTATAGTTTTGATACCTTGTCCCTGTAGCGTAGTCAAAGACTACCGGTTCCATTCAGAGCAGGGTTGCAATGTGCCCCATATCTGAAGAAGTTCCATTAGCATGCATCTGGAGGAAAGAAACTCCATCATCGCTCACTTTATCGGTTAGAGGCATTCTGTCATTGAGACCTCAGAATTTTTGAGAATCCACTGATGATGCAGACTCTAAGAAGACTAAAATCAGATTTTTTTTGTTATTTCTTCCTTTAACCTCATGGAAAAGTTCTTCAAAAGTAAGTGGATCTTTGTACTGTTGAGCTGCGAAATTAAGAAGCGATTTTGCTATTTTCTCGTTTCACTCTACAATATCCTGATATTTATCAAGGTAACGAAACGCGAGCTCTGGCTTTTGTTGAATAGCGATCTTGAGCAAAGGCTTTACAACGGTATCTCCCTGCTCATCTTTTCGATCTTTATAGACATCGATATAGCTAAACGCCAACTCTGGAGCCGCTGCTACCGCTGCAGAGAGTACTTCAAAAACATAGGAATCTCCGTATTGGTCTTTTGCTTTTTTGAGTGAAGAATAGCCAAAAGTCTGGAAAATTTCCTCGGGCTGTTTCTTTGCAATTTCAAGCCAGAAACTTTTTCTTTTTTCGAGGTTTTCTAACTTTATCTCTGGAAGATCGAGCTTTTTCTTGGTGGATTGGTCAGATTTTTCCTTTTGATCTTGCAATGAGAGTTCATAAGCGTTTCTGGCAACTTTATCTAGAATGTTTTTTTCTGGCAACTCTACTCCCAAGAATGATATCTGAGGAAAAGGTATTCCATACCGCAAAAATGATGCGATCATAACTCTGAGAAATAAATTTTCATTGATCTTTATTTTGATACTTTTTGCTAGGAGAAATGCTAGGAATACCACGAGCAAAAAGCTCATCAAAAAAATCATACTATACAGAAATACTACATTCGGACCTGCAGAAAGGAAATTTAGACCTGTCGTAAAACTCAATCTCTGTTGGAAACTCAGAATTACCAATAAATCGCCTCGATAGAGAGCATAAATTCAAATTACCCCAAGGAGAAAGAGTATTTTTAATATGAGGTATTTTTGCTTGTCTACAGCCCGAATTAGAGCAAGTGCTATCCCTAAAATAAGGAAATCTGACTGCAGCAAAGTAAAAAAGTAGTATAGCATATGCAATACTCCCATCTTTTGAAGGACGTAGGAATAAGCAACCGTGATGAGTAGGACTTTGATCACGATAAGCAAGATGAAAGCGATGAAAACAATCTTTTTGAGGAAGATATCTGATTTTTTTATTTTTTTGGGCATTTTTTCTTCTGGAAAATAAATTGAGAAAAGTAAAACTAATTCTTGTCCAGAGGAATAGATGGAGGAAGATAAAGAATCATCCCTGTTTCAAGCGGGATACTAGCTTTTAGAAACACCGAATCACTATTACAATCAGCTTTATCCATACTACGCCCTTGTTGATCTAGTATTTCCGTGATCGTCAAAACGCCTAAATCATTTGGAGTCATATAGTTCACCTGCATTCCAGGATGGATAGTTTCTTTTGGAGAGAGCATTTTGGCATTTTCTAGTCAGTTTTGGAGCTGATTTTCTTCTATAATTCCGACATAATTCCTTGCAAACAATGGTCCTGGAGTCTTTTCAGTGGTCGTCCCATCTTGCTTTAACATGCTAGGCAATTCTTGCTCTGAAGGTCAGGTTTTTAGTACTGTTTCGGAAGAAGCACTTTCTCCATCTGGGAAATCCTGCAGCTTGTGGAATAGAAACCCTTCTCGATACTCTCTATGTGGGATAACATTGACGAGATTCTGGATATCTGGATTGATTGGATGTCAGTGAATGAGTGCATCTCTTACGTGTTTATAGGCTTTTACCATTCCTCAAACATAAAATTCAGACTTACTCCTTCACTCGATTTTCATTGCATCAACATAAGGTATCAATTCGTTTAATCTATGGATGACACAAAGGTCTTTTGAAGAGAGAATAAAACTCCCCCCTTCTTCATTTTGCTCGAGCTCAAAGAGTTTTCATGGTCTCCTTGGCTCTTCTAGTCGGACTTTATATTGGAATCTGCACGCATGATTGCATTCTCACTTGTTCCCTGGCCTACCTCACATATAATCTCACAAAAGACATCTGCCTGAATAGGTCATACACATTGCCCCCTGCACAAAAATTTCGAGCTCCATCTCTGGAACTTTTTCTTTGATTTCTTTGACTTCATCAATATGCAATTCACGTGCGAGGACGATTCTTTTTACCCCGATATCTCTCCAGAACTGAACCGCAGAATAATTTAGCGTGGTTGTCTGGGTACTAAGATGTAAAGGAGTATCTTTGGCGTATTTTCTCAGAATATGAAAAGTTCCTGGATCTGAGAAAATAATACCATCAGCATTAAGATCCGCAATCCTCTCAACTACCGCCTCAAAAATCTTAATATCTACATTTCTTGGAAAGATATTCATCGTCAAGAGTACCTTGCTTCATAAGGCATGGACAGCATCTACTGATTCCTTAAGTTTTTCAAAATCTCTTACTTTATTTGATCTCATACGAAGGGAGGTAAAAGGAACTCCCATATATACTTCATCTGCTCCATATCTAGCTGCAGTAACAGCTTTTTGAAAAGAACCTCCAGGAGCTACGATTGCTGGAATATGCATTCAGTTTTTTTTCTTATAAAAGAATTGCCTGTTTTTCACGTAAGGGTGAGTGTATCTTTTTACTCTAGGAAAACAAGAAAAAAAATCTGATTTGATCGTCAATACAAAAATCATCACTCAAGAATAAACATAAACAAATATTTTTTCTCTTAAGCTTTGAGATTTAAGCGTTCCAGAATGGAAATATGAACAGCCTTTTTGAGTCAGAATTGAAAGTTTTTTGAAAAATATTGCTAGAATAGCTTGAAATACTGACTTTATTCAGATTTACAACTTTTTGAATTTCAGAAGCTTTAAATGCCTCCCGATATTAAACTTCGCTATTCAAAATAGTTAAGATTCTTTTTTTAGATTCCTCCTCTGAAAGATGCTTGGTTTCGCTGGAAAGTCGCTCTTGAGCGATCATTGCGATAAGCTCTAACAAGCTTTCCTGGTTAATAGGTACATTATCATGATATACCTCAGAGAGTTTTCCTAAATATTGTACATATTCCCTCAGCCCGTTTGCTGCTTCTCTCCATCCTAACGCTTCATAGAAAGTTACAATTTTAGCCTTCTCTGCTTCTTGGGCAAAAAAAGGAATAAAAAAACCAACTATTACATAAGTATTAACATCTTTCCTAAGGTATGTAACCTGGTCTTTTGGATTTGTTGTCTTGAAAAAGGTATTGGTGATAGCAGTAATCAGATTTTCATTTTTTATTTCCTTGATCAAAAATGCCAAAAACTGTGCAAGATGATGATTGATCGCCTTGTCTTTTTTGATTTGCTGGGCAACTTGCTTTGCCTGTTTTGCTTGGGTTTGCACACGTTGGACCGCTTCTTCTGAAACTTGATTGACATCACCTCTAAGCGGTCAAAAATCCTCCATACTTGACACTACCTCACTCATTTTCTCGCTAAAAAAATAAAGAAATCTGATTTTGGATCTAATGTAATGTTTTTTCCTTGAAATTCAAATCCAATTCACTATATAGGGAGTCAGCACGGTTTTGGTGAGGTGGATGAGTGGTCGAAATCAGCTCACTGCTAACGAGTCAGGCGAGAGATCGCCTCGAGGGTTCGAATCCCTCCCTCACCGATTGCTAAGATATGGCACTATAGCTCAGCAGGTTAGAGCGCGCCTCTCATAAGGGCGAGGTCGGTGGTTCGAGTCTACCTAGTGCCAATTATTAGGTAAAATGGATGATTAAAGCGTCCCGCAAGACTGCGGGAGTGATTCCAGTCTACCTAGTACCAGTTATTAGGTAAAATGGGCGATTAAAGCGTCCCGCAAGACTGCGGGAGTGATTCCAGTCTACTCTAATGCCGATTATTGTGATCATCTCAAAAGTCATTCAAAATATCTTTTATTTATCGAGTACATTATTATGGCCGCTAAGAAGAAAGGAGGAGTTGTTACTGTTGCAATGGTATGTAGTGAATGTAAAGCTACGAACTACTTTACAAGCATCAATAAGGCTACAACTCCAAAGCTTGCTCAGACAAAATACTGCAAGCACTGTAAAAAACACACAGAACACACTTCAAAGGAAAAATTAAAGTAAAAATCTGATTTTTCATTTTTAAATTTTTATTGCATTTCCAGCATGTTAACAATTAGATTGTCAAGACACGGAAGATCAAAAAGACCTTTTTATAGAATCGTTTTGACCGAACATACTAAACCAGCAAAATGTGGGTACAGAGAAGTCCTTGGATCTTATGATCCTCTTCATCATAAGCTTGAAGCGAATGTTGGGGCTGTTAAAGAGCGAGTTTCAAAAGGTTCTCAGATGTCTGAAAGAGTGGCAAAGCTTCTTTATAAAGAGACAAATGATAAGCTTTTCCAGAAGTTCTTTGAAATGAAGGATAAGAAAAGATTACCAAAGAATGCAGATAAGTAATTTTTGAGAGAATCCGTTAAGGATTTTTTCTTTATTTGTTTAGAGGATTATTTGATTCATGCCAGATATCAATCAGTACTGAGGATTTGAAGAAATTGTATTGCACCTTTTGGGAGGGATCGTGCTTATTATTTGCTGATGATGTATATATGCTTTCTTTAGGGCTATCTTTCTTTTCATATTTTCTAAAGGAGATGAGAAAGAAATAAAAGCAGCATGGAGTAGTATTAGATATATGATCTTATGAATATTTTTCACGGTGATGCTCCTCTTTGTCTCACCGACACTACTTAGATTCATGCATGTACAATGAGCAGAAAAGTATCAACCTAAAGCCATTTTCGTCTATATGGGGAAAATTATTTCTTATATTGGAGGACTAGGGAATATCATTAGAAAGTCTCAAACTACTAATGAATATAATGGAGATCTCTTCTACGATCTTAATAGTCCGACTTCTCTTGATAGTAGCAGTTTGTAACTATAGTTTTCTTTGAAGAGCATTATTTATACTAGCGTTTAGGTAGTTTAAACTAAGAAGCTGGGGGAAAATTCAAATACTGATTTGTTCTATGTGTTTTTGCTTCTCTCGATGGAGGACTAGTTTTTGGAGGAATATAGAAAAAAGAACAGGCAAAATCTTTTGAAAATAAGGAGGAAAAACATATAAGTTTGGAGCGTTTTTTGAACTTCTGAGTCTAAAAAAACTTATTTTTGGCTTGAGTTCGCTGCTGATTGAGTAATTTTATTCGTGTTTAATACTAATGCTTTTTGATGGTAAACCGCTTAAGACTTGGATCTTATGGTGATGAAGTTTTTTTGTTTTGTTTGGTCTGCTTTTTTCTTTTTTTTATTTTTCTCAGGGAAAGCAAGCCACTTGACCGGTTGAACAACCTACTATTCAGGTGAGAACTCAGACCGTTGGTGACTTTTCTCGCCAACTTACTCTGGAGAAACCAGGGACTATTTTGTCCAGTCAGGATATTCAGGTAACTGCTCAGGCTAATGGGAAAGTTGCCCGTATTCTTTCTAGGGAATGAGCTCTCGTTCAAGGTGGGCAGCCGATTGTCCAGCTCTCAGATACGATTGCAAGCTACAAATTACAAGCAGAAAGAGCTAAAAACTGACTCGATAGAGCGATCCTTACCAAAAACCAAACTGAAATTTCGATTAACCAACAAATACAGCAAGCCGAAAATGCTTATAAACAGGCCCAACAAAGCTTTGATCATGTTCAGAAAAGTTCAGACACTGCTATTAAGCAAGCTTGACTTGGACTTTCTGGAGCAGAGAGTCAATTTGAAGCGTTAAAGAAGAATTTTTTGACACAGAAAACAGCACTCCTCAATACGATGAATGGAGTCATTGATTCTGCAGATGCTCTGCTTGGTGTTACTCCTTATTATGAGGATCAACTCCGTGGACAAGAAGTCTATATTTGAGCGAAGAATGAAAGTCAGAAAAATCAGACCAAGGAGGACTTGAAAGCACTCTATCGTTTGAGAGATGAGATCAATACTCTCGCAGATACTCCGAGCGATGCAAACACTCTTCTTGACTCTACAAAACTCCTAAATCAAGGATATGAGAAAACAATCGCCTTTTCTAGAGCAATGCAAGTTCTAATGGAGAATACGATACCTTCAGAAGGGACTTTTTGAGATGCTGAGAGGAATAGGCAGCTTCAGCTCTTTCAAAACTACCAAGTTTGACCAGGGATGCAGCAAGCAAGATCTGCTTTTCTTGCTTATTCTAATCAGGTGGAGGGACAGCTTAACGGATCTTCTACTATGGATAAAGAAAAAGCTGACCTTAACTATAGTTCTACCTTGGCTAATAGTCAGAATTCTATCTTTTCTGCTGAAATCGCACTGAAAAATGCAAAAGTTAACTACGAAACTCTCCTTGCAAATAAAGATATTCAGCTTGACCTGCTTAATAATGCTATCACTGATGCGAAAATTGCCTATGAATCGGCACTGATCCAGTATAATAAATTGCAAGTAAGGTCGCCTGTGTCTGGAGTTATTGGAACGATTCTCGTAAGTGAAGGACAAGAAGTTGGAGCAGGAACACCAATCTTTAAGGTTTCAGGGACGAAGAAGCAGCAGATTGAAGTTTATCTCACTGCTGAAGAATATCACTATCTCTCTGAAAAAGAACCGGTTCTTATTAGCTATCAAAATCAGCTCCTTACTGGACTTATTGATGCAATGAGTTCTGTAGCTGATAGGACAAATCTCTTTAAAGCGACTATCCAGCTCGCTAGTGAAGTAGCTCTTGTTGGTGATGTAGCGAAAGTTAGCTTTCCACTTAAGGTCGCAGAAAATACGCTCCTCCCTCTGGATATGGTCAAAGTGCTTAATGAAAATGTATGAAGCATCCAGATTTTTAGCGGAGGGAAAGCTGAGTCTTACCCTGTAAAGATTCAAAGGATCTGGTGATCTTTTGTTGAACTAAAAGAAGCGCTTGATCCTCAGCTTAAACTGCTTCTAAACAGCCAAGAATAGGAGCCTGAAAGGGATTACTTTGGATTTTCTGGGGAAGAAGAAAGCGGTTCTTCCTTTGGAATACCTACTTTCTCTCTCCGATAATCTGATTTTTAATCTTTGCACCATTAAGGAAAATTACTGTATCCATATCTTCAGGAGCTCGATGCTTTCCAAAAAGCTCTAGCTGACTAGAATGATGAAAAGGAACAATATATTTATTGTGGAAACTCGTATAGGATTTATTAAACTCCTCCATGAAGTTTCCATTTTTAACAATAGGAGAAACCAGAAAAATATCTTTATTCGTGAGATGAAGATCGTTGAGCCATTTACACAGGTATTCTACATAAAATCTAATACTTTCTAAGACGAGATTCTTGGCAAGAGGGTTATGCTCGACTTCCTCTGCACTCTTGTATCGGTATTTCACGATCTGATTGTCCTTGTACATCTGCATTAAAAAGTTGATTCCAAAGTTGATATGCTCAATCTGCTTGTAAAAACCATCTTCTACTGCGACAACCTTACAGTAATTTTCCTTGATATACAAGAGCAGGAAGGCATCACGACCTAGTTTCCTCTTGAGAAAGGAAATTGTTTTGAAGCTCTCGGGTCGCAGGTGGAGATTCTTTTGGTTAAAGACATTTCCATACGCCTTATTAAATTCGAGAAGGGTATTTCTATTGATATAGATGAAGTAAAGACGGAAGAAAAGCTTTCCTTTGGCTCAGATGACGAATTTCTGAGGCTCTCCATCCACAAAAATTGTATCAATATTGGTAAATAGCAGCTCCTCTTTGGTAATATTTCTGAGGTATTCCAGTCTCTCCTCAATAACCGCATTTAAATCTTCTATCGTAAAAACACTTTCCGTCTCTTTGTAGATATCGAAACGAAAGAAATGAAACCTCCCGTCATCGAGGAGGAAATGATGCTCATACTGAGAGTGGCGTGAAGAATCATCAATAATCTCAGCGAGAGATTCATACCTATCATACTGCTGCATCCCCAACCTTTCTTGAAATTTCACCACGCAGAACTTGGGATACTTCGGATCGTAAGCTTCTCTCCAGAGATAAAACATCAGACTCCTTATAATATATAAAAAATCGAGGATTGTAGGAAGGGAAGGTGCTAAAGATTTCGTTTTCCGAGAGGTTCGACTCTAGCAACTCCTCTAGATAAGTGCAAGGAAAGAGGGATTTTCCGATCCAAGCTCCTCTTCTTGTTGCCCCTTAGGGCTTGTATTAATAATGCTCTAGCGCTTTTATTCTATCTTCTATGCTTGGATGAGTTTGAAAGAGCGAACTCATCTTCTTGAAAGGGTTTGCGATAAACATTGCTGCCATTTCTTTGTTGTCAAGCTTGACTTCTGGGCGTGCGGAAATCTTTCTAAGTGCAGAAATCATTGCTTGATTGTCCTTGGTAAGCTCAACACTTCCCGCATCAGCGAGATACTCTCTCTTCCTCGAAATCGCAAGCTTCACAAGCGGATAGAAAAGGTAGCCGAGCATCAGCAAAACGAGACCAATAAGTGGTAATACGTTCTTGTTTTTGGAGTTGCCGCCGCGTCCTCCACTCCTTAGCAGGATCTCTCCCAATAAACTTATCGCTCCAATATAAAGCACCATCACGAGCATCAAGAGTGAATCCTTGTTGATAATATGCGTGAGTTCGTGGCCTGCAACGGCTTCAATTTCAGACTTTTCTAGTGCATTGAGCAAGCCTCTTGTAAACACAATCCAAGAATCTTTTGGTCTCCAGCCTAGAGCAAAGGCATTCATTCCTGGTGTCTCAATAATCCCAATCCTCGGTGTTGGCAATCCTTTGGAGATGCAGAGGTTTTCAATCATATTATAGAGCTTTGGCTCCTGGGCTCTGGTGACCGGCTTGGCTCAAGAGAAGGAAAACATGAGCTGCCTCTGGAAAAAGAAGGAGATTAAGAGCCAAATCATCAAGACTGGAGCAAGTAAAGCTAAGGCTGCTCCCGTTTCTGTTAGTGCGTCTTGCATTCTCTCTTCGGAAGCAAGGCTTGCGACCCTCACTCCTCTGCTATCCCTATACGTCGTCCCGTTTGAAGCAAAGAGAAAGACGAAAAAGAGCGCAACTCCGAGCATAAGCGGAAAGAGCACGATTAAGAGTCACGTCTTCATACGATTGCTTCGGATTGCGTGTTGAAGTCCTTGCATGAATAAGAGTGAAAAAGAGATAAATCTGATGTGTATCTTATGGTTATGCGGTGAATTTTCAAAGGATTTTTTGAAGAAGCGGTAGGAATGAGGAAAAAAATCTGACTCTTTTGTTGGTTTGGAAGGTGATCCAGAATGGTAAATGAAAGAGCGATGAGAAAAGGAGTGCCCTTTTGAGTGCAAGATGGCTTGGCTACTAAGAGGAACTCGCCTTCCTGAAGATAATTTTTTGTTGCTGAGTGAAGTGAAGGATATGAGGTCATGCCTAATAGGGTTATTTTGCTTTTAGAAGTATCGAAATGAGGGCGGCTTCGTTCCTTAGAATGACCAACTGCAACGCATTTGCGGGTAGCTTTCCTTGCCCGATCCCCTGAGCGTGAAAAATGGGCTCCATTTTTGACTCTCACCCTGCGAGACTCTCAAATTTGGGGGACAAGATTTGATCGCTGGATGCCGTGGAATGAAAAATACCCCCTGTTTTTGACTCTTGACCTGGTCGAGGATATGATTTGGGGGGTCAAATTAGGGTGTCGACCTCCCCGGAGGGGTAATTTGGGGGGTCAAATTGGGGTATCGACCTCCAGGAAGGGCAATTTGGGGGTCCGTTTTACCCTTCCGACCACCCCGAGCATCAAATTTGGGGGTCATTTTCGCCTTTCCGGCAGCTCGGAAGTCAAATTTGGGGGGTCAAATTAGATCAGCGACCTCCCGGAAGGGTAATTTGGGGGTCATTCTTGCATCATCGACCTGTCCGACACCCTAATTTGGGGATCATTCTTGTATCATCGACCTCCCGGGGAGGCTAATTTGGGGGGTCAAATTGGACCAGCGACCTCCCGGAAGGCTAATTTGGGGGGTCAGATTGGACCAGCGACCTCCCGGAGGAGCAATCTTGGGGGTCGAATCAGACTTTTTACCTGTTTTACCCCAAAAACCTTTACTCACTATTTGATAGTCGAGGGGATCGGAACCCCAATATTTTTTGAAAATAAGGTCATTGACCTGCTGGACAGCCTCAGCAAGGGGGCAGGATTTGGGACTCGAGCAGGTCGGGAATGGGAACAAGAGTAAATGTCGGAGGTTTTGGAAGCTTGGAAGTGAAAAGCAGCTCCTCCTCTCCCTCCTCTCAGGATAGGAATTCTGGGATTCGCAAGTTTTCAAACAGATAAGGGAGAGAAGAAACTGAAGAAATCCATTGAAAATACAAGCCGAAACGCTACACTGGGAACGGAAAATATGCAGCTGTTTATTTCTTTTTTTTGAGAAGCGAATGAAATTGAGTGTAAATAAAGCTCAGAGATATGCAAAGATGAGAGCTCACACGGCAACGCATCTGCTCCATGCTGAGCTTGGAAAGATTTTTCCTGACACGAAACAGGCAGGGTCTTTGGTAGATGATGATTTGTTGAGATTTGATTTTTATGCGGGAAGGCTCTTGAATCCTGAGGAGATTTTGAAGATCGAGCAACGTATCAATCAGATGATTGCTTTAGCGTGTCAGGTCGAGACGGTAGAGCTTTCGATTGAAGAGGCTTCTCAGTTGGGCGCAAAGATGTTCTTTGAAGAAAAGTATGGAGACGTCGTGCGTGTGGTGAAAGTGCTTAACGGAACTAACCAGACTCAAAAGGCTGACAGCCAAGAAGGGTCAGATTTTTCTGATTATGGGGAGTTTTTGTCCTTGGAGTTTTGTGGGGGGACGCACGTGAAGAATACTAGAGAAATCGGGGCGTTTACGATCATTTCTCAGGAAGCGGTGGCCTCTGGAGTGAAGAGAATTGTCGCGCTTACGGGACCAAGAGTGCTCGAGAAGATCGAACAGCAACAAGCCTTGTTGACCCAGCTCGGGGAGCTTTTGGAGGTGAAGTCGTTTGCGCAGTTGCCGGAGAGAGCGGCGAAATTTTTGAAGGATTTTGGGGAGATGAAAGCTCAGCTAGGAGCGGTTGAGACGAAGATGATCCATGCGTTATTGATTTCTGGCGACAAAAAGTCAAATCCTGACTTTGATGTCATTATGAAAGTGCCTGTTGAGACAAATTTTAAATTGCTCGGGACGGAAGCTAAGCAGGTTTTCGCTGGCAAGAACGTGCTCTTGGCGACGACTGCTGGGAATTTTTTGCTTTTCACGCAGCCTTGAGTATCCGCAAAAGAACTTGCACAAAAATTAGGGCTAAAAGGTGGAGGAAACGATATGCAAGTGCAAGGAAGAGATGAGAAAGTAGTAGAGCTTCTTTAGGTTTGGCGGAAAGAGCGAAGCTGCTAGAACCAAGAACCCAAAGCAATAAGCAATCGAACCAAGATAAGAAGCGAATAAAAGTATTTTTCACTTGAGAAAAAACTGAGCCAGAAGAAGGATCAGTTTTTTTTGATAATTCTGAGAGAGCTAGATCAGTGTCGTATCTCGAGGTCTTCGTTCTTTGTAAGGGAGCTGGTAAGCGTTGTCTTTGAGAATGGCATTATCAAAGAGATTGGCGAAGAAGTTGTATTTATTTTTCATAACGGGAGTTTCGCTTGCCATCGTCCTAAAGAGTTGGCGGTTGTTGAGGAAGAGGTTGTGGCACTCGGCAAAATCTTCAAAAGGATTGGTCATACCGTAGCCACTACAGAAGTCTTTTTTGTTGGCGGATTGGCTTCTGATGTTCTCGGAGTCCCAAGAATATCTATAGTATTCCAATGAAGGATCGTCAATGGCGAATTTTACTTTACCAAACTCGGTATAGTTTCCGTTTTTAGTTTTGCTTATGCCGTTAAGCACCCCGAGGTCCACGATATGTCCAAATTCATGCGTCAAAACCCCCCGAAATTCCGTCGGGTCTCCCATAGAAGAGAGATTGATGGTGATTCTTTTTCGGGTGGCTCCTCCTCTTCTCTTACCGGTTTCACCGTTGATGATCAAGGTCTTGAAAACGGAGTGAAGCGGATCGCCTTGCATAAGGTTTTCCTCTAAGAAGTTTGCTATTTGAAAGTATTGGTTTGCGTAGGATCGTTTTTCTGACTGCGTAAGGGTTCAAGTTCGAATAATTTTCTCGCAGAAAAGCTGATTCTTTTGGCATAGGGAGCTGAAAGGATCGTTTTGTGGCGATGCTGATGGGCTTGGATGGTCAGGCTTTATCTCTTCTGAGAAATAGTCCACCTGGAGCATAGTAGAGGGTTCGCCTGCTTCTTTTACCCCAATGACTAAAGCCAGAAAAAACAAGATCGAAGCACCGATGAGTTTCCGAAGGTCAAAGAGAGTAAAGCTAAAGAAGATTTTCCTGTCCATATCGTAAAATATCATTAAAAATCAGGTCCTGCTGATTTCACTTATTATTATATACAATCAATTCTGAAATTGCAATTTTCTCAGCAAAAAGTTGGAGCAGAAAATTATAGCCTCAAATGATGATTTTCGTGCCCTGATGCTTGGCTTTGAAGAGGTTTTTCCCGCTTCCTCCTGTGATATTCTCTACTAAGAGCAAAAAATTCTGATCTAATCCCCTATCAATCATCGTTTCAAAGAGTACTTTACTTTCCTCCTTAACCACAGAGAGGATGAAAATCGTTCCGTTAGGAGACTTTTCACGATCAAAGGCAAGTATTGCCTGCAAGACAGAAGGGGTTTTACTCAAGCGGTGGATGTTTGCAGGTTTTTGAGCGTTCGGTGCTGATGGAGCTGGAGTTGTTGGCTTTCTTTGAGGTTCTGAATCCGTCGGAAGCGGAGAATCAGAGGATGTTGAGTCAGGATTTTTGTCTTTTATGACTGGAATGGAAGAAGCAGAGGAAGCTGGAGCACGTGAGGCTTCGGGAATGAGGGCTGGGAGCTGTTCGTCGGTGTGCGAGAGGAAAAAGATTCTGTGCCCGACAAACTCTTCTAAAAACTCCTCCCAATTGGTAAACTTTACCTCTTCTGCATAAACAAAGTAGAAACCTGACTTACTATACATCTTCTTTTCTACAATCATCATCCCCTCAAAAATAGTCTGCATATGCTCGATCTGCTTCCGAATACTCGTAAATTCACCTGCTTGTAAGGTAGCTTGTAAGCGAGGTTCTCGTTCTTTGAATTTGGCGAGGAGGAGATTCGTCTGGTAGAAGGAAAGATTTCATTTCAAAGGGTCTAGGGTCTGGGTTGTGGCTTCGGTATGGGTAAAGAAGGCTTTCGTTTCCTGCCCGAGAATCCCCATAAAAATCGTAAAAAACTGTCTAAACTCCTCGACGGTTGCCAGATTTTGAACTTTTTCTTCTTGGCCTGGGGAGATTTCCTGCTCAAGGTGGTATTTATAGCTGAGCATATCAAGATAATTAAGCGTATAATTGAGATCATAGGGGCGAGACAGGTAGAAATTATAGCTTTTATACCACCGCTCACAGTCAAAGAAGACAATATCATAGTTTTGATACCTTTCCGGCTGCTCCAAAAGCGAAAAAAGCCCGCCATGTGTCGCTAAAATGATCGGGTAATTGACGGTAAGTCTCTGATCTTTGACCGCAGTGTAGATTTCATAGTCGCCCTTACTATTAAGGTCTAAAACTCCTAATCCCTGCTCAAGATGAGAGAAGTATTTGAGCAAAAAAGCGAATTCTGACTCCTCAAACTGTCCTTTATTTACGAAAGCTTGCAAGGCTTCACGATTCAGGGTTTGTTCTTCTTTGACGAATCCAAGGTTCTTGACTCCGAGTTCATTGAGGAGGTTTTTTGCGATATCAAGCTTGGAGCGGTTGGAAAAGGCAAAAATGGTCTGTTTATTGGTAGCGAGCGAGGAGAGAAGCTTTTTAAATTCCAAATTTTTGACCTGAAATTTCTTGCGATTGAGGTTTTCCGTCCGAGTAAAAGGGTATTCTCCAGTAAGGATTTGGGTATGAGGAGCTGTAATTTTCTTGAGAGGAGGCAAAACGAGCGGTTGCATTCAGCTTTTTTCTCTTTTTTGGAAAAGATGTGCGAAAGTGCCTTTACTTTTGGCAAGTAACTCCTGCAAAGCAGGATACTTTTCGATCAAATCCTCTACTCTCTTCACGAGATACCGAAAGAGTGCGATACTCAGCTTCGCATCAAAGAAAGCATCGTGAAAGTTCGCTTCTTCTCAGAGGGAAAGCTGAAGATTCTTCAAAATTTGCTGAAAATTAGCCTTAGATTGGAGGCTTTCGCAGAGGATTTCTAGTGCATAACTGGGAGCGTAATGAATAAGCGTTTGAGCCCAACGAAAAGTATCAATCTCTGCTTTCCAAGCTATGCTTGGAAAGAATTTTCTCAAAAACTCCAGATCAAATCCGATATTATGACCAATAATAACCGTCTTATCTCAGAAAAAGGTTGAGATTTCCTGCTCTACTTCAGCACGCGTAGGGGCATGTTCTAGCTTTTCTACTGAAAGACCAGTGATAAAGCTGACAATACTTTTTAATTCTTTGAGCGGTTTCTCTGGACGAATAAGCGATTGAAATCCGCCAATAATCTTTCCCTCGTTATTAAACTCCACAATTCCAATCTGAATGGGTTCATCGGTCGCGGTATCTAGCCCAGTAGTTTCAAAATCTAAGGCAATCATACGAATATCACTTCTGAGCATTGCTATAATAGATAGGGTAAATCATAGTCAAGATTGGAGTATATATAAATCCTTTTTGAAATCAACCATCACCCGAGCTTGATTTACCTTCCTTTACCTTCGTTTTGAACTACTCTTTTTCAGCAACCAACCAAATAAAATAAAAATCTTTAAAAAAAGTTGACATTTTTAATTTTTATTATATAATTTTATCGTCAATGGCAGGATAGTGATGCTATGACACCTCTTTCCTCTGGAAAAGATTCAGATTTTGATAGTGTTTCTTGCTCCTCTCACCTTGAAGAAGGAAAGGAAAAAAACTGATCTTTTATGGAGAAACTGTCTTCGTGATGAAAAAATGGGTTCTACACACAACCATTTATTCGTTAACAAGACACCATGTACAACCTCAGAAAGCCAACTGGTCGATGAGGTGGTTTCTGGAAACTTTTAGTTGTTTTTGTCATTGGAGGATATACCTTGACTTCAGTGTTAGCTTTTGATTCTCAAGGAAATATATCCTTGGATCAAGGGCAATTATCAGAAAAACTACAAGCTTCCCAGCCACTTCACGCTCAGATTGAAGAGAATGTTGCTTTCGGAGCTAGGAAAGTATGGAAGAATTTAGTGAATTTTATGCAAGAAGGATTTGATACTAAAGAAGGAGAAAAAGGTGAGAACAATTGATCTCTCACTGGAGAAACCACTTCTCCTCTTGTCAAAACTCCCGAAGGCAAAAATCAACCAATTATCCGTACGAACTTCTCAGAAAGTGAGTCTGTTTTGTTACCTCTTTTTGAAGATATTGCAAAAGATCCTCAGAAAGAAAGTATCGAAACACTTGCCAGTATTGGAGTTGTAAACTGAGGAGCTAATGGGAAATATCAACCACTTAATCATGTGCGTTGCAGTGATTTTATTAGAGTTGTAGTTGATATTTACAGACATAAAGCTGGATATTCATTAGAGAGCACCAAAGGGCTCACTGATCAACAGCTTTTGTCGTTGAAAAATCCGGATGCTCTCCTTAGTAAAAAACTCAATACTGCGAAACACCTTTGATTTCTTGAAAATGTAAACGCAATGATCCGAGATCATACGATTACACCGCTTCAAGCACAGCTGATCCTTAACAACATCTTCACACTTTATCCTCATCTTGGTCAAAAAGAGAAGATAAAACTGATTGATACTACGAAATCAGTTTTAACCAAATCTGAAATGGCCGGATACCTTGTGGCAATTTTCCAATTGGAACCCCAAGAAACGGATAATCTATTTAGAGATATTCATAATCATCGCTATCAGGATGCAATTATTCAGCTTGCCCAGCTCGGGATAGTAGCTGGGAGAAACGGTAATTTCTACCCTGATAATGATACGCTCCGTGCAGACGGAGTAATTATGATTGCAAACAGTATGCTTGCGAAAGAACAAAAAGCTCTTGTTATCAAAAACTTTTATCATCTAAATAGTATTGCCGATGTCACCTATTTTGCAGCCTTTGCTCCCCATTTGGAATTTTTGCTTGATCATAAGATCGGAACTTCTTTACTTCGTCATGAACAATCAGGAGATTTGTTTCTCCCTTATGCTCTACTTACTAAGTGAGAAGCTTATTCACTTATTACCCAAGCTGCTGGAATTAAAATTCTGAACCCTGATGCTTGATCAGCCACCAAGCCGATTACAAGAGGAGAGTTAGCTCAACTCCTTGTAGAAGCATTTGATTTTGCGCATCTTGATGCAAAATCTGAACCTTGAACCCCAGAAGAAACGTCAGTACCTACTGATCCTGAAAAGAAAAAATCAGTCTTTGTTTCTCTGCTCAAGGATATCGTCAATGAACTTTAATATCCACTACATAGCAACTGCTTCGGGTAAATACTCGAAAAAAACTTGTGAAATTACAAGGTATTCTTACCACTTTTATTCTCTTATTTGACACCAGATGAACACAACTAACACACCAAATCTTTTGGCTCAGGAACTAAGTGCAGAAGAACTTAAGCTTCAGAACGATGAAGAAACAAGAAACTACAAAAACAAAAAAGAAGAACTAAATATCAAAGACAGATTTGATGTATGGGACACACACTTGTATGGAGAACAGGTTAGTGCAACCTTGAATGCAACCAATAGTATCGCTTCAAGTGCTAGAGTGGCTAAGGCTAAAACTCAAGGAGACCTCCTTCTTGCTCTTCTCTCAAAAAAACCTCTGGATGCAGAGGAAGACTATGCTCAAGCTGCCTAAATTTAAAAAAGCTCTAGTGAAATTACCTAGAGTTTTTTTATTTCTCTTTGGCTGGCTGAGATTTTACTGATCTCTAAAGCCAGAGGAGAAGGCAAATCAAGCTGGTCTTCCCACTTTGGTCGCTCTATCGCAATCCAATCAAATTCAGCAATCTGATTTAAAATCCCTTTTTCTACAAGTTCGGAAAAACTTGAAAGCCTATAAAGATCTATGTGTAAGAGCTTGTTGTCGTAGATATTGATGTAGGTATAGGTAGGACTCTGCACTTGATCAGGATTAATTCCTAGTCACTCAGCAAAACCCTTGATAAGAGTCGTTTTCCCGGCTCCGAGTCCGCCGTGGAGCAAAAGGATTTTAGTCTCTTGGACAAGAGATTTTCCGTAGTCGTGCATAGCTTGTGGAGAAGAGATGAGCATTTGCGTTTGAGGAGTAAAAGTTTTACTTGAGAGTTGAGTATATGTGAATACTTATCAAAAGCAAGAAAAAATCTGACTTTCTCTTGTAGTTTTCCCCTAGATTGCTACAATCTGAGTCAGGTTTTTTAGTTTTTATGTCAAGATGCGATGAAATATCACTTACAAATCCTGAGGAAATATCTCTCTCTAGATACAACGCCTCAAGATATCGCAAAAAATTTGATTCTCAAGACTTGTGAAATCGAGGAAGTAATAGAAAGGCAGATCCCTCGCTCTATTGTGATCGGGAAAATAGAACAGGTCGAGAAACACCCTGATGCTGATAAACTCAATATTTGCCAGGTGAACTGCTGAACACAAGGGAAATTTCAGATCATTTGTGGAGGAACGAATGTGGCTGAAGGGCTTTTTGTAGCGACTGCTCTGGTAGGCACGCTATTTCCTGAGGCAGGGATTACGATTGCAAAAAGGGCAATGAGAGGTGTAGACTCTGAAGGGATGATTTGCTCAAAAGGAGAGCTTGGTATTAATGAAGATAAGGATACCCACTGGATTTGGGACCTTGCACAAGACTTGGAGGTATCTGATACGGATCTTGGTCGTCCTCTCAGCGAAGTATTCCCTCGATTGGAAGCTACGATCTTGGAAGTAGACAGTAAAAGCTTGACGAATAGGCCAGATCTCACGGGACACTTTGGAGTTGCGGTCGAGTTGAATGCTATCTATCCAGATGCTTTGAAGAAGTTTAACGGCATCAAAAAGTGGATTGAGAGTTTTCGTGATACCAACATCTTGGAGGTGCTGGAACATACAGAAAAACCGCTTAACCGTCAAGTTCGTGGGCTCTCTAAGGCGGTCAATACCTATATTGCCCTGGGAATCAAGAATATCGAGGTCAAAAAATCAGATTTCTTTTCTCGTTTACAGGTTTTGGACCTTGGGAGTAATCCTATCAACAATTGGGTAGATTTTTCGAATCTTTTTATGAATACGGTTGGTTTGCCAATTCATTGCTTTGATGCAGATAAGATTGAAGGTGCGATTATTATTAGAGACGCAAACGAAGGAGAGCTTTTTACGGATCTTTTTGGCACGGAGCATCGCTTGCAGGCGACAGACCTTGTGATCGCTGATGAAAAGAAAGTGCTTGCACTCGCTGGAGTGATTTGAGGACTAGAAAGTGGGATCACTGACATGACGAAGAATATTCTCGTCGAACTGGCGAACTTTGATCCTGTCGTAGTGAGAAAAACAGGAACAAGACTCTGACTTAGAACTGATGCTGAACTGAGATTTGAGAAGAATATTAGCCCAGCCTTTAGTTTGTATGCATTACTTTTGTTTCTGGATGAGCTTAAATTTTATGCGAAGGATTTGTGAAACTATGAAATCGAGGGCATCGCTTCCTATATCCGTCCTGACATCAATCCTCTTGAAAAAAAACTCATTCCTACGCCTTGGAACACGTTGCAAAGCCTGATCTTTGGTGCGGAGCAACCAGATTTCCAAGAGAAAGCAAGCGCAATCCTTGAGGGGCTTGGGTTTTGAGTGGCAGGCGATGTCGTGAGCGTTCCCCTTTGGAGAGGACCAGATGATATCAACATCAAGGAAGATGTTGCTGAAGAAATTGCTAGGATCTGGGGCTATGACCAAATAAGTCCCCAAGCTTTGCTTTCAGAAGTGAAGGCTCAGCCGTTTAGTGAGAGTGTCGCTATTCTCAGAGCGGTAGAACAAGCGATGGTAGAAATTTTTGATTTTGATCAGGTAGAAACCTATCCTCGGGTGGCAAAAACTCAAGTGGAACTCTTGTGAAACACTCCTGATACGCTCTATACGCTTCAGAACCCACTCAATCCTGAGTTTCCTTATCTAAGGGATAGTATGCTTTATAATCTGCTCCCTATTGCTAGCAAAAGTAGTAAATTCTTTGATCATTTTAAGATTTTTGATCTAGGGAAAGTATGGAAGCCTGGAATTTCTGAGCAAGCACTCCATTTAGACCAGAGATATGCCGAAGAACACTTCTGCGAGGAGCTTACTCTCGGAGCATGCCGATACGAAAAGTCCTGTGCAAGCCGATCTGACGATCCGCTCCTCAAGATGAAAGCTTTGCTTACAGCTCTTTTTAAGAGGCTGGAGATCAAAGGCAAACTCTTCTTTGAACCATCAGCTTTCAGCTGTTTTCATCCGAAAAAACAGGCAAAACTCTTGCTTAGAAATGGTGAAAAATTAGTAGAAATAGGACAAATCTGATCCTTACACCCTTTCGTATTACAAAAATCCAAGCTTCCAGAACAGGCACAGCTCTGCACGCTTTCCTTAAACCTCGAGCCACTCAAACACCTCCTCCACAGTGCAAATGCAGAAAAGAATTACGAGACTTTCCAGGATCAAATTGTGTGGAGAGACCTTTCTTTTGTAGTGGATGCGAAGACTTCTTTTGAAAAGGTAATTACGACGTTAGAGAAGATGCCCCAAATTAGTAAAGTCAGACTTTTTGATCTCTATCAAGGAGAGAACCTCGGAACCGATAAGAAATCCCTTGCGGTGCAGATCAAAATTAAGGGTGATGGTGCGATGACTACGGAAGCCATCAATGCCGTAATGCAAGATGCAATCAAAAAGGTGGAAGCAACAGGAGCTCAGCTAAGAGCGTAAGTTTTATTTCTCTGCTTACGCTATGTCTTATCGTAACTTATTAATTATTATTTTGATTCAAGCTTCGTTGGCGATGCTGGGGTCTTTGTATTTCTCTAATTTCTGAGATCCATTGAATGGGTTGTTTTCAGGGACAGGTTTTGAGCCGTGCCATTTATGTTGGCGAGGAAGAATTTTGATGTATCCGCTGGTTGCAATTTCAGTATATGGACTGATCAGAAAAGATAAGGGAATCTGTTTTTTGACAGGGGCTTTGTCGCTTGTTGGTGTGCTTTTGGCAGGATACCACTATTTGATTCAATATCAGGCATCGCTTAATGTATTTACCTGTCAGGCTGGGAATGATTGCACACATATGGGATGGCATATTGGATTTGTAACAATTCCGTTTCTTGAGCTCGTGGCTTTTGCGGTGATTCTGACCTGTAGCATCCTGATGCTTGTCAGGAGAAAGAAATAATAACTGCTCTATATAAAGGAGAAAACTGATCTTTGAGGTCAGTTTTTTTTATTTTCCTCATCCAAAAGCTTTCAGAGCTTCTATACTAGGAGCTGGCAAACTTCTGCTGGATAAGAAGTAAAGCTACAGAGCCTCTTCACTGGTGAACGAACGACTAAAACAAGGCACTAAAAAAGAAATCTGGATCACGGCATCTCTTTTGGTCGCTCGCAAGGGAAGGTGGTGGTTGTTGCTTCACTAAAAAATGACGTTTTGAGATAAGCGGACCCCTCCGCTTCAAGAAAAACGACGTTTTAGGACGAGCGGACCCCTCCGCTTCAAGAAAAACGACGTTTTGGGACGAGCGGACCCCTCCGCTTCCCTATTACAAAGCGGAAATTTCAAAATTTATAGCTTTTTTATTTGTTTATTGAATGAAAAGATGACTTCAATTACAAAAATCAAGGCAATTGCATTAACAAGGCTTAATAATCTTGAATAATCCTGAATATGGACCAGTTATGGAGTCTTTATGGACTTTTTTACTGCAAATTTTCTGCATAAAAAAAGCAGCTGAGCGCTGCTTTGCTAGGAATAGGAGCGGAGGAGAATTTAAGCGCCCTCGATCTCTGTCCCATAATGGATCACGGTATAAACATATTTTTTGACAGGATCAAACTCGAAGCCGAGTCCCATCTGAGTGAAGTGTGGCATCATTAACGCTCTATAATGGGGTCAGCCTTCTCACTCGGCGGCGAAAGCGTCAAAGATTTTCTTGGTAGCGTCGATAAGCTCCTGGGTGCAATCTTGACTAGAACAGCGATAGGTTCTATAGCCGATACTTTCTGAAAAAGAAGATTTTCCAGCTGTTTCTCCAGTAAATTTTACACCCTGATCTGAAAATCGAACCTTAATAGCGGGATAACTATAATATCCGTCTTTGGCGGACCTTTGGTGCGTTCGTCTCCCGAGGGAAACGAGATAATCCGCTCGCTTTTTAGCGGTTCTCTCCAATTCACTCTGATAGCGGTAAGGTTTCAGGTTTTTCTTGGCTCTCAGGTCGTTATGTCGTCCAAGTCGTGCCGTTCTCACTTTTTCAAAGTCAACGTTTGGCAACGTTTGGTAGGATTGCTGAGCAGCGGGAGTCTGGGTAGGAGGAAGTCAAGCAGTTGCCTCTACTACTCTCTCTTTGATCCGTTGGTCCAGCTCTTGAGCGATTTGTTGCACCTCAGCAGGAGCATTGCTCTGGAGTGTTGGCAGTGTAAGCGTATTTTTGAGCGTTTGTAAGTAGGTTGACTGCTCTTGAACGGTCATGGTTTTCATTTTTTGTTCGGCGGAAGCGATGAGTTGGTTGGTTGCATCACTATAAGCAAGTGCCGAGATATTTGTCAGTAATAAGCTAGCGACTAGAAGTAAAAATCAGATTTTTTTTGTGCAGTTTTTGGTAAGCGTTGATACGTTTTCCATAGAGACTATTTACAAATAAAAACTACTTCATTTTTTCAAAGAGGAAACCCATTCCATCTCAGAGTATTACAGATTTCATAATAATTCCTAAGTAGAGTGGGATTCATGAAGTATTTTCGTAGAGTTCTGCGCCATCTTGGGCATACACCTCTCACGAATTGATTTTTTCAAAGTTTTGATAGGGTTTTGTATAGGTAAAGTTTTGTGTTTTTGGATCCTTACTATCCAATGAATGGGATTTTGATACCCCGTCGAAAAACGAGGTCTTTGAGGATAATCACTACCCCCAACAGCATCAGTGCTCGAAAAAAGACAAAGTTTATTCGTCCTTCAACTGTCGAGAACTTTTCGTCACTGATGCGGAAGAGTTTTTGCAAGATTACTGCCCAGAATCTTCCTCCATCTACGGCTGGGATTGGGAGAATGTTAAAGATTGCCAATGCAAGAGAAAGCATTCCACCGAAGCCGAGGAAAATCAGTGGTCCAAAAGCATCGAAAAGTTTTTCTCCGAATTTCACAGCACCTACAGGTCAGGTTAGTTGATTAAGGGCTGATTTTGTATCGTTTGCGTTGAACGAAAAGAGTTTTGAGCCGATACGTCAAAGGGAAGTCATCGTAAGGTCTCGCTCTGCTTTTATTTCTTTGAATCCAGCGAGCATCGCTTTGCCGAGTCAGAATTTTACAGGGAGAACTTCAATATCACCATCTTGGTGGAAGACGATCCCAAGTTTACAATCAGACTCACAAGAGAATTTCCCCTCTTGGAGAGAAGACATCTCAAGCGCCTCTGCAAAATGGATGGTATTCTCATTGGTATTGCTGAGCTTGGAGAGGATTCTATTGATTGTAAGGGTTGAGATTGGTTGACCATTAATATCCACGAGGACTGCACCACTTTTGATACCAAGTGAGGCTGCGATACTACCAGAGAGCACTTGTTCCACAACTACCCCAGTGTCTTTGATCTCACCACTCAAAAATCCCTCAGATTTCAGGAAGCTTATGCTCGGCATCAGGTAACTTTGGGAAGAAATCCCTTCTAAATCATCTGGCAAGACACTGATGGGTTTCGTCCCAATGAAAAAGAGCGAAGAAAAGACTACTCGAGCAATCAAAAAGTTCATCATCACTCCCGCTGAAATAATGATGAGTTTCTTTCGGAGTTTTGCCTTTACGAAGCTATTGGCATCGGTATTTTCTGCGGAATTTGTGCCGTCTTCTCCCTTGAGGGCAACAAATCCTCCGAGTGGGATCAGATTTAATGTATATTTTGTGCCTGATTTATCGGTTCGTAGTGTGGCGATTCTGGGTGGCATTCCCAATCAGAACTCCTTTACTTGGACTCAAGATTTCTTGGCTGCTATGAAATGTCCGAGCTCATGCAAAATTACGAGCAAGACAAAAGTAAGTATTCAGAGTATAATTCAAAGCATTAAGGCTTTCTTTAAAAAAAATAAAAAACCTGAGTGAAACTATATGCAATCCCTTCCAGAAAACAAGCAAAAAAATAAAGGGAGGATTTTACTCCCTTCGGATGCCGTCTAAAAGGTTTTTTAATTGTGGATTCGTTTTGGATTTTTCGATCAAAAGTGTCTTCATCTGTTCATCAATAACGAAGAGTGAAATACGTTTGATGAGACGCTCATTTGCTTGGATGTGAAGGTAGTGTAGCCCTGCTGTCTTTGGAATCACGGTAACCGTTCTTCCCTGTCCGAGATAGGAGACCTTAGAAGGGAATATCTCTGTCCCAGAACTTCAAGTAATTGTAATGTCATCCTTGAGGAAAACTTCTCCTTTTGCAATAGGAGGGGTGATTTCAAGTGTTATACCACTTCCTAGCAAAGCGATTGGAGACTGGAGGGTATAGTTTAATTCTTGGGGAAGCATTGAATTGATGAGAGATTCAGATTTTTTTGGCTGTTGTTCTGCGAGAAAGATTTCTCCTTGCGTCTCCAAAAAGAGGATGGGATCTAAGGTATTAGCTTTTACTTGGTTTCGGCACTTCGCCTCATTCACCACTTCTGCAATCGTCCCGCTACAATTTTTGGGAAAGTAGGGATGTTCTCCGTTATTGTGATCTATCTGAAAATGAACGTGAGGACCTGTAGCATTCCCAGTCTTTCCCACCTTTCCGATAAGCTGCCCTTCTTTTACCTGCTCTCAAACCTTTACAGCAATGCTTTCTAAGTGGGCATACACGGTATGAAGGGTCGTACCTTTTCGATCATGTTTGATGACAATGACCTTTCCTCGTTCTCCTTTTTGCTGGGCATAGACCACTTCTCCATCTGCCGCCGCACTAACAGGCGTACCCTCAGTGCTTGCAATATCCACTCCTCCATGAGATCAGAATCAAAAATCCCGTCCTCAGAAATAAGTTCCTCCTCGCATCACTGTATATACCTGATGATAGAGCGATTGTTGCTGATATTTGGTATAATTTGCCTTTTCTATGCGAGGGAGAGTGATTTTACAAGTTCATTCTTTTTCAGGGCAAAAGGAGCCTTCTATTTCTTTGAGTGGATAGCTCCATCCATAGACCACAGGAAGCGTGAGTAGTATAAGTAAGGCTATTAATAGCTTTTTCATCAAGAACTTTAAAAGTAAAAGAAGCTGATATTCAACCGGCGAGTAAGTATACGCTTTTCCTTTTTGAAAACAATAAAAAAAACTAGTGAAGAAATCTGATTTTTTTCTTATAGGTATGAGCCCAGATTTTAGTTAATTGCCTATTTGGGAAAGGAGAAAGTATTTAAGTATCTCCTATCGCAATGAGAATACTAACCATTATATTGTACACCTACGCACAACAAAACGAAAATACTCATGTAATAGACTTTTCTCATGCTTGTGAAAAATATAAGAGAGCTCCAATACACACACATTGCTCTTGATCAAGAAAATAGACTTTATTTGATAGGATACAAACTACACATAATCATTTTTTATTTATGAGGAGATTATTCCTGATGGATTCTTAATGTCGATGCAATTCTATACAATTCAGAAAGGAGGGTTGGTTTTAGAATATCATAATGCCTCAAGATGAGTGATTGATTATTCCTCAAATAAGTTACCCTTTGATCAATTCCTGTACGATACTTGGTAATTTTTAGGAGAAGTGTTTGAAGTGATTTTTTGAGCACTCACTGCTCTGCTAAACAACTTCTTGCTCATTTTGCTCATTCTACCGCTAGATTGAATCAGTTTTCTTGATTATCTTTTAAACTTTGGCTGAACAGTTGGTTTACTGTCGTCATTCTTGATTGACAGAGTGACAATTGATTTTCAGTGTGTTGCAACTCGCCTCTTATACGATTTTCATGGTACGAGAAAAGGTTTAAAAGCGATGATCCGTCTGAAAGGTAGATTCTCAGATTTTTTTCTCTCTGTGATGGAGATTCTAGTAACTGGGCGATATCTTTTCTTAAAAAACTTTCAGATTCAGAAAAAAGAAACCAAATATCATTATTTTCCTCTGGTCATCAGAGGTCCTCGGTCATTGTGATTCCCCCATAGCGAAAATGCAGTCTCATAATCGTATTCAGATCAGCTAAGGTCGTATTTATTGCGAAAAAGACTATTAGTATTCAGATAAGTCAGCAACATTTCTTTATCATTTCCTAGATCTGTGGAGAGATAAATCAACTATAGCCTCTGGACAACTTCCATCATACTTCCCATTGGCAGGAAGATTGAAGCTACGATAGTTCCCACAATGGCTGCCACAAACATCATCAAAATTGGCTCAATCATTGACATAGCAACATCTATTGAAGTCTTGAATGTATTACGGTAGAAATTAGACATTTTCCCTAGGACATTAGTCAATGATCAGGTATTTTCTCCAACATTAATAATTTGGACAAGGATAGGATCGAACAAGGTAGATGAAGCTAGTGACTCGTAGAGTGTAAATCCAGCCTCTAAATCTGATCTCACATCCATCATCTTTTTTTTGTAATGAAAATTATCAAAAATTCCTCCTAGAAGATTAAGTGAAGTTACAGGACTTACTCCAGCTTCGTAAAATTGGGACAACAAGCTCGAAAACCTGTACATATAGAATGTTTTTACAACATCTTTAATAACTGGTGTCGTGATAAAAAGCTTATCTATAAAAATCTTAAATAGAGGAAGTTTTGCGTAAAGCAATTTGTATCCCACGATAATTAATAAGAGGATTCCAAGTAAAATAGACCATTTTGCCTGTAAGAATCAAGAAACCTCCATCATAAATTGAGTAATTCATGGCAACTCATTGTCTCCAAACATTCCTACGATTCCTGGCATTACGTGCACAAGGATTACGATTACTGCTCAGATCGCCATGAGGAGCACCATAGTAGGATAAGTCATAGCTTTTGAGATTTTTTGATTAATTTCTTGAGTATTTTCTAACTCATCCGCAATTTGCCCCAGAACACTCGTCATATTTCCAGTTACTTGTGCGGACTGTACTAGTTCAATTTCATCTGAATGAAAAAAGTAATTATGGTCTTGTAATGCTTGAGCGAGAGATGCACCTTCGGTAAGAGATACGATCAGGTCTTCAAGAATTTCCCTGAATCCTTTATGAGTTTCTGATTTTTTGATAGAGATAAGAGATTCTCTAATTCCCAATCCAGCATTTTGGGATACGGACAAAAGTCTAAAGAAATTTACCTTTTGCTTCAGGCTTGGCTTATTGAGCTGCTGAATCAATGAATCAAATCAGTTTATTTTCATTTTTTCTTTAATTGATGTAAATTTACTTTGTATACTCAAAACCTTTTATCTTTTATTCCTCATCCTTACGTATCTATATAGCTCATCTAAAGAGAGATTTCCGTGGATAATCTTAAAAATTCCATCTCTTTCAAGATCAATCATTCCTTTTGCCAGAGCATAATGCCCAATATCAAAAGCTGATTTTCCATCCAAGATCAGCATTTTTATATCGTCTGTGTAGTCCATCAATTCGTAAATTCCGATTCTTCACTTATATCCTGATCCTCCACATACCGGACAAGTATCTCAGGTTTTAGGATCTTTTCCTGTTCCCACAGATACGATTCCTTCTTTGATGAAGGTATTTCGTTGCTGACCTGTGATTCATCTGCTTATTAATTCATCTTTCAAAGCATCTTTATCAAACTCTTTGAATGTTTCTAGAGCATTGAGATACTTAGGATCAGTCTGAACGTTGATCTTAGTTGCACAATGTGGACAAATTTTTCTACAAAGTCTCTGTGCAATCACGAGATTAAACGTTCCAGCAACCATAAAAGGCTTTGCTCCAAGATTAAATGCTCTTGTTAGGGTTTCTACTGCTGAATTCGTATGAATTGTTGAAAAAACAAGATGTCCCGTCATTGAAGACTCCATCGCCATTTCTAGTGTTTCCTTATCACGAATTTCTCACACCATGATTACATCAGGATCCTGTCTCAATGCTGATCTTAGTCCTGAGAGGAACGTATATCCAATATCTGATCTTACCTGAGATTGGTTGAGTCCTTCCATTTTATTTTCAACTGGGTCTTCATAAGTAATGATATTCCTCTCTATAGTATTAATTTCATTTAATGCTGCATAAAGTGTTGTTGTCTTTCCAGATCCTGTTGGTCCTGTATTAAGCAAAATCCCATTCGGATCCAAAAGATGCTTTTCAATTGTGACCTTATTTGATCCAACGATTCCCAATTGATTGAGCGCTGGAATTGATTTTGATTTATCCACAATTCTCATTACAAGTTTTTCTCCCCAAACGGTTGGTAGTGTATTCGCACGAAGATCTATTTCTTTATTTGTCTGGGTAATACTGGAAACTCTGGCATCCTGAGGAAGCCTCTTCTCATCCGGTCTCATCTGTCAAGATTCAATTTTAAACTTGGAAATTATTGATTCATGAAGAGAACGAGGATATTGGATGAGTTCTTGTAGGACTCCATCTATTCTTAGTCTAATTCTTGAGTAATTTTCTAGTGGTTCAATATGGATATCTGATGCACCTTCTTCTACAGCAATTGTCAAAAGATTGTCAAAAATCTTCACGATATCTGACCTAAGGATAGATTGCTGTAATTGTGCCTTAAATTGTACAGTTTCCATACCATTATCTGTTTCATTAAACAAACTAATTATAAACTAAAAAGTAAAAAAAGCAAAAAATAAGAAAGTTTTTTTAGATATCCAATTCCAATAATTATTCTCTAGCAATCAATTTATAAACTATTCAGGTTTCATGGTTTGTATTTTCTCTTGCATTTTTCTTTGACTTTTTTATAACTACGTAGCACCTGTTTAATTTATGTGATACCACAAAAAACAGGGGCATATCATCTGATTTTTTATTTTTTATAGATAAATCGTATGACAAGACTTATTCCATTAGAGGATCACATTATTGTGAAACCACTCGTCCAAGAATCAACAACAAAATCTGGGATTATCCTTCCAGAAAATAAAGAGAAGCCGAGCAAATGAGAAGTTGTAGCTGTCGGAGCTGGTAAAATCCTTGAGAATGGCTCTAGAGCGTCTGTTGATGTAAAGGTTGGTGACATTGTGTATTTCGCAAAATATTCTATTGATGAGCTAGAAATTGATGAAGATGGAGAAAAAAAGAAATATCTTGTAATGAAGCAGAATTATATTATGGCAAAAGAGGCTTAAATTTACCTGTTTGTGATCTATGTTAATGATGAGGAAATATTTTTTGGGGATGATGGTTTGAGCATTCTTTTTGGTAGGGAATGTTTTTGCTGTGGATCCGGTAGACACTTTTAAGTGGGCGTATGACCGCGGAATCCTTTCTCTACATTCATGAGTTTTGTGAAGTTCGGTATTAAAAAGGCAAGAAGTTGCTCCAATTTTGCTTAAGTATATTTTGGAAGTATCTAAAAAGGATTATACTGATCGTGGATGTAAGGCGTCTGACATCTATCAAGCGGATACTGCATATCAGAATGATCTCAAAACTCTTTGTGGATTTGGTATTTTTATTGGGGAAAAGAATAAACTCCTTCCTAAGCAATACCTTTCAAATGCTCAGGCTATAACACTCGTAATGAGAGTCGTAGATGGCTATCAAAAGGAAGCCACCTGAGGAGAGCATCGAGCCGCTCCTTACTTTGAGAGGGCGAAAGAGCTCTGATATGAGTGAATTACTCCTCTTTATTACAAGAAGAATCTGCCAATGACATTGGAAAACTTCATTAACTTCCTTTATAGCGTAGAGCACCCTCATCAATCGGTTACTCAAACAGAAATGAGCGTAGACACCTCAAATCAGAAATCAAGTTTTAAATCATCAGACGATGCTCTACTGAAGCTCGCTGAGATTATGTCTAGTTAAACGACGACTTAGGAGATAGAAAAATTAGTTAAAATCAGACTTTTTATTTTGATACATATAATAACAATGCCAAAACACATTAAGTATGGTGATGAAGCCAGAAAAGAAATCTTTAGCGGAATGCAAATGGTTGCAGACGCCGTGAAAGTCACTATGGGACCAAAAGGAAGAAATGTAATCCTTGAAAGATCTTATGGAGGACCTATCGTAACGAATGATGGAGTGACCGTAGCCAAAGAAATTGAACTTGAAGACAAATCCGAAAACATCGGAGCAAGCATGCTCAAAGAAGCTGCTGAAAAAACCAATAAAGAAGCAGGAGATGGAACAACTACCACCGTTGTGCTTGCAGAGGCGATGGCGAGCGAAGGACTCAGATATATCAGGTCTGGAGTGAATCCTTTCTCTCTTGGGAAGGGGCTTCATAAGGCGGTTGAGAAGCTTGTAGCGGAGATTGAAGGGAAAGCCCAGCAAATCGGAGATAGTAAAGAGAAAATCAAGCAAGTAGCGACAATTTCTGCTCAAGATGAAGAAGTAGGAAATCTGATCGCTGATGTCTTTGAGGAGATCGGAAAAGACGGAACCATTACTGTTGAAGAGGGAAAATCTCTCGGTCTAACCAAAGAAATCAAAACAGGAATGCAATTTGATCAGGGATATTCTAGTCCTTACCTTGTAACTGATCCTCAAAGAATGGAAGCAGTAATTGATAAGCCTTATATTTTGCTTACCGACAAGAAAATTTCTTCTATCAAGGAGATTTTGCAGGTTTTGGAGGCAGTTGCTGCGACTGGGAAAAAGGATTTTGTGATTATTGCTGAGGATGTTGAGGGTGAAGCTCTGGCTTCTTTGGTTTTGAATAAAATCAGAGGAATGCTGAATGTTTTGACCGTGAAGGCTCCAGGATTCGGAGATAGGAAAAAGGAAATGCTTCGCGATATCGCGGTAGTAACTGGTGCAACAGTGATTACAGAAGAGCTTGGACTTAAACTAGAGGACGCTACGATTGAGATGCTTGGAAGAGCTGATAAAGTGATTGCGACCAAAGATACGACCGTAATCGTAGATGGTAAAGGCGATCAGACCGCTATCAATGAAAGAGCTGATCAGATTAAAGCACAGCTCTGAATGACCACTTCTGATTATGATAAAGAAAAATTAGCTGAAAGGCTGGCTAAACTCGTTGGTGGAGTGGCGGTTATTCAGGTCGGAGCTGCAACTGAAATGGAGATGAAAAACAAGAAATATAAGATTGAAGATGCTTTGAATGCAACTAGAGCCGCTATTGAAGAGGGAATCGTTGCTGGAGGGGGAAGCGTATTGCTTCAGCTTTCAAAGCTTTTGGATGGCTTCAAACTAGAAGATGCAGATGAACAAGTTGCTGTGCAGATCGTGAGAGATGCGATCCAATATCCTGTTCGCCAAATCGCAAATAATGCAGGTTTCAAAGGAGATCGAGTAGTAGAAAAAGTAAAGGAGTCTGAAGATTTCAACTACGGTTTTGATGCCAAAGAAGGAACATTCAAAAACCTCTTTGAAGCAGGGATTATTGATCCAGCAAAAGTGCTGAGAGTTGCACTTCAGAACGCGGTTTCTACTGCTGCGATGTTCCTTACGACTGAAAGCGTGATCGTAGATGCTCCAAAGGCAGAAGGCTGCTCTTGCTCTCACGGAGGAGCTGATGCAGGAATGTGAGGCATGGGCGGAATGTGAGGTATGTACTAGAGTTAAAAATATATCTTTATTACCTGAGGGAACTGGCTTTTGGAAGTCAGTTTTCTTTTAATTGTAAATAGATGGCTTTTCACTATAAAAATTTTATTTATTTGTTTGGTAAAAGATGAAAATCTTGGTAGGACTTTCATGAGGCGTAGATAGTGCTGTCGCGGCGTATCTTTTGCAACAAGAGGGACATGAGGTCGTTGCCGGCTTTATGAAAAACTATGTTTCTGACAGAGGAAACTGTACTACGTATAATGATTCGGTTGACGCAATAAAGGTGGCTGAATTTTTAGGGATTGAAATTCGCTCTTTTGACCTTCAAAAAGAGTATCAGGAGAGAATTATTGACTATATTTATGCGGGATATCAGAAAGGTATTACTCCGAACCCAGATGTACTTTGTAATTCTTTGATCAAGTTTGATGTTTTTTTGGAGAGAGCTTTGGAACAAGGTTTTGACAAGATTGCAACCGGTCACTATGCTAGAATTAAAGAATATCAAAAAGAAGATTGAAAAACGATGTTTCATCTCCTTCGTGGAGTGGACCACCATAAGGATCAATCTTACTTTCTCGCTGGGTTGAATCAATTTCAGCTTCAGCATTCGCTTTTCCCTCTTGGAGAGCTCACAAAACCGGAAGTGAGAGCGATTGCTGAAAAAATTGGATTGCCAAACGCCTACCGTAGAGATTCTCAAGGGCTCTGTTTTGTGGGTAACATTCCGATTAAACAATTTCTTGCACAGAAATTTCCTATTAACAAAGGGGAAATAAGAACCTTGGAGGGAAAAACGGTAGGAACTCATGATGGAGCTTACTTTTTTACGATAGGGCAAAAACATGGATTAGGACTGAATTTTAAAGCTTATGTGTATCGTATTGATGTAAAGGAGAACATCGTCTATGTTTGCAATAAAGATGCTGATGAGCTTCAAACTCAGGAGATTACCGTGAAAGATTGGCATCGAATCGGTGAGGCTTATCCAGAAGGCGAAAAACTTACTGGGAAAATTCGCTATCGTCAGAATCCCCCTGTTGATTGTTTATTATTAAAAAGAAGAGAATCTGAATTAATTATTCGTTTTGATTTTCCTCAGCGGGCAGTTGCTCCTGGTCAGGTTTTTGTCTTGTATAATGGAGAAACCTGCCTATGAAGTGGGATTATTGCATAGTAAGAAACGCTTCGTATCTATAAAATTAAATAAGCATACCTGCATTTTGTTTTCTTTAAAGGAGGGATTGATCTATTTTTGGATGCTTTGAATAAAAATGTCTTTTATTTTTTGCATTTTTTCTCTTTGTCAAGTATAATTGGACTGAATTTTATCAGACAATTATAAAGAAAAGACTATGGCAATCAGAAGAATAAGAAGATTGAAATCAGAAAGGTTAACAAAACCGAATATCCTTTCCTTTATTGCGTACACCTTAATTTTGTTTTTTGGAGTTACGATCTTGGTAAACATTAACCCTAATGCAAATGAATCTTATCGCAATAAGCGAGATGAGAAAAATACTCAGGTGATGCAATGGATTTTTTTGGAAGAAAATGGACTTTCTCATATACTTTTTGGGGAGACAGTTACTGGAGAACATGCCGCTCCACAAACTCGAGACTTTCTTCTTCCAAAGTCTAGTATAGAACAGCAAGAAAGTAGTCCTGAGGTTGAGAAATGATCGTTGTGATCTGATGATCTTTTTGCATCTTGAGAAGAAGACAGAGAACATCATGCAGCACCTGATGAAAATTCTGATTTTTTTTCCTGATGAAAGGAGTCTTGACAAGATTCGTCAGTTATAATGAGCTGAAATCAGATCTTAAAAACGCCTAAAAAAGCATATGGAGCACTTTGGAAAACTTTGGAAGATCGTTCAGTCTTTATCAATATGATTTTTGAATATTTGATCAGAGGAGATATTGTAGAGCTTCCTGAAGGGGCGGTCAGATATGTCGTTTTACTCTATGGAATGGATGATAAAGTTAAAGAGAAAGTTTTTGAGGCAAAGTCCTGCATCACACCTCGAGGATATGAAATCGCTCATTGAGGATCTGTACTTGCCTATGAACAGAGAGCTGATGCTCCCTATGTTTGCAATCTTGAAAGGAGAACCTGCAAAAACTGAAAACTTTCTGGTAGTTTTACTCAAGCCTCTTGCGATGAAAATCTCAATGGGAATATCAAAAAGTATTCTCACATTACGACGAACACTGAAGCAGCGATCACTACCAATGAATATATTCAACCTGATAAAAAGGCAAAATTTGAGGATGCTGATTTTAATTTGCAGGGGAAGCGTGCACCTAACGAAAAACCTCACCTTATTTCTTGACCACTTAGAAATGAGACAAGTGCAACTCCAAATGATGTTCCATTAGAACAGACTCCTCGTAAAAACTGCATATCACCTTGGTGAGAAACAATAAAGGCTGGACAGTTTACAAAGGCTTATAGATTTCAAAATGGATTCACTGATATTCCTTGCAGAGTTCAGCTTAGGCTTTGCGTCGATGGAGAACTTGAATGAACCTATGAATATCCTACTTGTCAACCTTGGGAAACCTCTTATGAAGACTTCCTCAATGGCTATATGAATGGAGATGAGCCTTCTCCTCAGAGACTCATAAAAATGCTTAGCTCTGACTTTACTCCGGAACCAGAAATGTGAAATAATCTTGCTCCAGAAATTATTGATAAAATGTTAAGGATTCTAAAAGAATAAAAGTAAACTTTGGGATAAAAAAATACCTCTTTCCTTCTGAAGAAAATAAACAGAGAGAACGAGGTTTTTTGATATAAACGAAGATAGGTGCTATGTAAAGCACCTTTAAACCCATGAATTTATGCTATGGGAAGCGATTCTGAAGGATCTGACTCGATACTCTTCACCTCCCCCTCTTTGAGCTGGAAGCCGGAGATAATCAGCCCAATTCCAAAAATGGTAATGGCCACGCCTAATATGATATTAAAAAAGAGAAGGCTAAGGAAGGAATAACTCACAAGGAAAATTCAGAAGAGAATCGCAACTCCTCCCATAAATAATAAGACTCGTCGAGTTCCAATCTGCATATCTCTCGCTTTGAAGCCATCAAAAATGGTAAGAGCCCCCTTTGCGATAGAGGCGATTCATAGGAGAATAATCAGAATTTTCATTAATAACTCTCAAAGCTGAGGAATTACTAAAAGCAGAAGTCAAAATCCTGTCTGCACTACAGATCAAAAGAATAAGATTCCTCTATAAGAATAGTCTTTTTGGAGTCGAGCGATTGCCATTCCTGCGCTTCCTGAAAGGAGAAGCTCTATTCAGAAAATAATAATAACAACTCTTAGTGCAAGTTCTGGCTGAAAAAACAGGAAAAGCCCTAATAGCATTAAAATAATACCATTAATTCGGAAGAAAGCTTTGATCTTAAGCATACTAGAAGAAAGGGATAAAATTACCTTCTACTCTATTTTTTATGAAAGAGAATGCAAGAGATTTCTTATCCTTGAAAAAAGAGAGTAAAACACTATAAAAAGTCAGATTTCTCTTTTTATTTTTTATTCTGCCTATGAAACTAAAGAAAATTGTAATGGGAATTTTATTCGCGACACTGATTATTGTCGGAATAGAAGGGATGATCAGTTTTTGGGAGAATCATAGCTACTATACGGCAATGATTTTGTCCTGAAAAAATTTGGCATTGCTCCTATCTTCAGCATTAGCAGCTCGAATCCCAATTTCTTTTATTACCAGCAAAAAAAAGCTGACCTTAAAGAAACTGATCCTTGGTATTGGAGCTGGACTTTGAAGTTTTTCCCTCCTTCATGCAATCTTTTCTGGGGGGCTTTTAACGCTCTTCGCTGCTGTTCCTCTTCTTTTCAATACGTTTCTCCTTTACCTTTTGGCAATAGGCTTCATTCTTGCTGTTTTTTCGCTTGGGAACTTCATATCAAGGAAGCTAAAGCTTTTTCAAAAGATAAGATGGCAAGAAACGCTTCTCACCTTTGGACTGGGACTCGTGACTTTTCTTCTCCTGATGCAAATCCTGATGGGAATTCAGCTTTTTTATAGTGTCGTAAATCGAGTAATTTTTGGAGGGCTTCTCGTGCTTATGCGATTCGAGAGAAAGGCTATGAAAGTTCATGAAGAGAATCTTATTTCTTGTCTAGAAAGCTGGAAATCAGCGAATAAAGCTTCAAAAATCTGATGGATTTCGGTTGTTTTGCTTCTGATTTCGTTTTGATATTACTTTTTTAATTTTTCTCACTCATATATTCCGTATTCTACTGCTTGGGATGCGAATCACGAATATATGTATGTCCCTAAGGTTATTGCCGAAAATGCGGGTATTCTTTGGGGGAATATAGGTCCTGCTAATGGAGTACAATATCTCTGGCAGGGATACATCTCCTTTTTCTTTTCTCTCTGGGCACCTCTCGGAAATTTCTTCAATATCGCAAAAGATACTGTTGCTATTAACCTTAATGCTTTTAGCGGGGTTCTCGTGTTAATCTTTTGACTTGGAGTGCTCAAAGAAGCACTCCTTGTCTTCGGAAGAAAGGAATCCTCTGAAGAAACTGATCAGACTTCTTTCCTTGTAGGACGAGGACTTCTTCTGATGTGGCTTACAAGTGGGATGGGAGCATTTCTCGTGTTTGTAGATAATAAGACCGATATGGGGGTTCTTGCACTGACGATCCTTGCTTTGTTTGGAGGGATGATTTTTCTTAACCATTTCCAAAAGAAGGGAATCGATACTGATTCAAAACAAAGTTTGAAATATCTTATTCTCTCTGCTCTCTTCTTTGGATTCGCTGTTATGGCAAAACCAACCGCTTTTATTGATGTTGTGATCTTTGCATTGTTACTTGTTGGCTTTTGGCTCAATACAACAACCCTTGTAGGAACCGGCATTGCAACACTTGGGCTTATGGGAGTTGTGCAACCACTTTTTGCTTCAGCATTCATTACTCCTGAGCTGGGGAAATGGCTCTTCGCTATCGGAATAGTACTTGCTGTCATTGGAGGAATCCGCGGATTAATCTCAAGAAATGATCAGTTTTCAAAAGGATTTCAGCATATTGTAATCTGGGGAATTACACTCATTGCAACGCTATTTCTTTTCAAGTGACCATGGGTGCTTGTCTCTAGCATAACTGCTGGTAACTTTCAGATTTGAACTTTTATTAAAAGCACTCTCCTCGCTCAATCTACCTCAAAAAATCAAGCAACAACAAACATAGCGAAACAGCAACCACTTCTTGCACAAACTACTGTGAGAATCAATACCGATAGACAGTCTGATATTGATCGTGCAGTGCTTAATCGTGCGACTCAAGATCTTGACTATCAGCAATGCCTCAAAGAAGCAAACGACCCTCAAGAGCTTGATAGAACAAAGCAACAAGCTCCTGGAAGCTCTCTAAGTGAGGATGTGGGAAGATATGTAGGATTTTGATGGAGAGAATTTAAACACACAGGGGTCGGTTGAGCTTTACTAAAACTTGCCTTCTTGAAAGAAAATACTTGCTTTGGTTGGGATAGTGATGGACTTCTCCTCTGCAAGAATCGTAACCTTTTGGACGCGAAAAATCTCTCTGAGATTGAAGCACTTTCGTCTCAACTTTCTAAAAATGGTGCTGCTCAAGCTTTGGTGAAATCGCTTCTAGATGCACAAGCCTCATGAGAAGACCTTAGAGATGAGTATAAGGCTTTAGAGACTTACTATCAGGAACATAGTATATTAACGACAACAGGTAGTGTATTTATCCCTTATAGATATATTGTACCTCTTAATATCGTTTTTAATCGGTCACTTCAGAACCTTTCTAGCTATTATACTGATATCGGACTCATCTGGCTTTGCGTATTTGTTGTGATTGGTTTTTGATTGCTCTATGCAATCCTTAGCTATGATAGGAAGTATAAGCATCTACTCTTTCTCAGCTTTGGAACGGTAATCGGGTGGATCATTTGGTGGGCTATTGGCGGAGGAATCCTCTGGTATGGATTAGGTCTTGTGATTTGGTCTACGCTCACTGTCACGGCACTCTTGCAGGAATGGGCCCCTAAAAAAGAAAGTGGCATAGAGTTTAAAACAATATATGGGCTGCTTGTTGGTTTCTTGGCAATCTTCCTTGCTATCCAGGCATTTATGAATGGTATGAGGATTGCTTCTCAGGCAGGTGAATGACCATTTGAACAATACAAGTCTAACGTTGGAGAAAAGCAGGAATTCGGAGATAAGCTAGAACTTAAAAATACCATTAGTTATAAATTTACTGCAAAAGACATCTTCAATATGCAGTTTGGACAGTATCAGCCATTTCTTGAGGCGGTAAAGAATAGAACTGCTGATGAAGGCGTCCTTATTGCCGGAACCTACCTTCAATACTTCTTGAATAATCAGAAAAATCTGAGACTCGATGGTATGCTGACTCGACTCTGGGAAGAGCTGTCAGACTTTAATAGCTGTAGATCCTACCAAAGACTTAAAAATGAGAAACTCAAATATCTTATCATTGATCCAAATATTGGAACGGTTGGAAGAGCAGGAGAAGGAAATGAAAGCCTTTTCTATAGGTTCTTTGCGAGACTCACCTCAGATGAGCAGCACATTGAAACTCATGGAACGATCAGTATGCTCGTAAAGATGGCACAGGAAGGCTACCTCAAGCTTATTTTTACAAATAATATTGGTGCAAAGTATGCCTTTACACTCTCTGATGAAGAGTTAACAAGTGTTTTTGGAACTCACTCTGCTGAAGATCTGCTCCTGATTAGAGCAAAAATGGCGGTTGCTAGGTTCTTCTCTGAGGATCAAGATCTCATTTCCAAAATCTATGCCCTCTTTGGACAGAGACTTCCTACACAGAAAGGGCTCTGAGATCTTGCAAATACTCTTCAGAAACAAGTGGATGAAGAAAAACTCTACCAAATCTTCTTACAGATGGCTGGAGGTCAATTAACAAATGCCTCAAGTTTGACTCAGGACGAAAGAATTGTAGTAAGTCAGTATTACGGTCTAATAAAAATGTTGAGCGATAAGACAACCACTGAACAAGGACAGAATGCACTGTTTAGTCTCTTCCAGAAGAGTCTCGCAGGAAGCAGTCAAATTATTTCTCTTGAGCTAAGATAAACAAGAAAATATTCAAGAAAGGAGAGTGGGTTACCTGTCTCCTTTTTTTAGTAGTGTAATGGGAATGGTAATGGTAATCAAAAAGGAGAAATCTGAAGTCAAAGAAAGATAAGACTCTATCAGAGGAAGTCTAAAGAGTTGTAGAATGAGAACCGTCTAGCAGCATTGATTCAGATTTTTCTCCTTGACTTTAGATAAAAAATCCCTATTTTTTGCCCGCAATGAAAAAAATAAAAGATCGAAGCCACTGAAGCCTATGATTTGTTTGGATTTTAGGCTTCTGGAGTTGGGAACAGACTCTATTACTCACCTGTTCCACTGAAGGAGGCAGACTTCATTTCTCATTGTAAAGTATATTCACTGAACTAAGCCCTGCTCTGTCTTTGTTCACACTAATAAAACTATATGAAAACCAACAAAAATTCTCTGACTTTTCCGCACTCGATGTTGGAGTTTGCTATCTGCTCTGTTTTTCCCAGAGGAAACCATATGATAGTAGAGGTATTTGATTTTGGTTCCTCTGAACAGCTAGAAAGTATGGAGATACGCTATCAGACTTTGGAAGAAGCAGATCTTCTCATTCCTGCTACAGCTGTAGGAAGTTATATGCCAAATGTTTTGCGAGCAGCAATTGCTGGGGATGCTGAGGGAGTGAAGGTTATTACTCGACATATCCGAGATATTCCTGGCGAACCTATCACTAAGCTGATCGTTCTCCTGCAACGTTTCGGCTATCAGAAAATCAAGACAGCTCCGCTTATGCTTCCTGAAAAGAACGATATTATCGTTTACCATGGCAACTATGGTCACGCTATTGATACTGATCGTGGTTGGATTGGTATGATTACTAAGGTTTCTGTCAAGCATGGGCAACTCAAGGTTTGTGCGATCGGAGCTCAACGCTGGAATGTTCAAGGATCGGCCGGAGATGAGCAGTATGTAAGCCTGATCACCTCTTATCGACTAAACCTTAGCAAAGTAAGTGTTTATCGGGGATACTCCTCTCGGTAATTCTTTAGAAGAAGAACTCCCTAGGTTTTCCTAGGAGAGTTTTTTCTTTTTTTGAAGAGTTGTAGGTATTTGATAGTGATCGGAAACAAAAAACCTTTTAGCTTCTCTTGACATTTCATCAAAAATCCATAGATTCACCATACAAAAAATAAAGTAAAAAAAAACTGAAGTCAGAATTATTAATTTTACTCTAGGCTTTGGAAACTGAGCGTGCACGCTATTTGTGACTGCTTGCATGAAGGAAGCAGACTTCATTTTTGTTGTAAAAAATAGTAATACCTGAATGCAGTGCTGCTCTCACTCCATTCACAATAATAAAATTTATATGAAAACTAACAAAATTTCGTTCGTATCCATTTGGATAGTAATGGTGATGGGGTTCTGTTTGAATTCCTGCAAAGAAAGAGATGTTTTACATTATTCTTTCAGTTCTTATGAGCGATCGCAGTCGCTTATTCCTCCCTGTTCTCAGGATGTTAACATTCCAAAGTGGCTGACAAGTAGCATTGCTGGCAATCCTGACTGTATTGATGTGGCCTTACATCTACTAAAGCTTGATCATCAGATGAAACCTATCGATTTCAAGCCGATATTGGAGCAGTACAACTATGAAGAGCCTAAATTGAAGTCTAGGAATGATTTAAGAGAAGGGGATATCATTCTCTATCATCTTGACTACGGGGAAGCGATTAACCCTGAGTGGGGCTGGATTGGTGTCGTCTACGACGTTTCTTTTGAGAATAATGGAGTAGCGCGTATTAGCTCTGTCGGTGCATGTCGCTGGAACTATAAAGCAACTGCCAACGAAGGTGAGATGGTATGTCTGCTCACCTCATATCATCTGAATATCCGAAAAATTAGTGTTTACCGGTGGAAGGATCCCCAATGATTATCAGAAAAAAGAACTCCCTAGGCTTTCCTTGAGGAGTTTTTTTGATCACTAAAAAGACTGGAGAATGAAAAGCCTGTGAAGTGAAAAAACTACCTAAAAGCTGTAGATTAACTAAGTTTAAGAGATTTTGAGGCAGTAATGATCTCGTCGAAAGGCTATCCGCGTAGTAATATGCTACTCTAAAAAACACCATATCAAAATCCCCCTACTTCATCTTGACATTTATCTATAAATATGTATAGATAGCAGTGTCAAAATAAAAAGCAAAAAGATTTGAAGTTTGTTCCTTCGATGAGGGGAGGCTTCTGGAGTTGGGGACGGACTCTATTACTCAGACGTTCCATCGAAGCATTCAGTCTTCACCTTTATTACTTATATTAACCACAACCTTGATCAAGACACTTCGTATGTCTTGTTCGTAAACAATCAAATGTATGAGACCTAACAAAAATTTAACAATCTCTTTCGTGATTTTGGTAACCGTATGTCTACTTCTCAGTTCCTGCTGGAAAGAAGACGTGACTACTATCACCCGCATTCCTCAGAAGCAAGCTGCTAGCATGCTTGAGGTCTCTTCTGTTCAAGAACTGGCGCCTCAAATTTTGAGGGGTGCCGTTATTGGGAACGCTTACTGCGTTGTCTATCTTCGAAACTCCTTCAAGCTTGATAGTGCAGTATCTCTCAACCAGTTTGTTTCGGTTCTGAAGCACTGGGGGTATGTTTCTTACCCTGTTTCTCTTCGTGATATTCCTAAAAAGGGAGATTTCATCTTCTATCAGCCTGATTATGGAGCTGGAGTTCCCAATGAAGGATGGGTTGGCATCGTAATTGATGCTAACATAGTCAATAGAGATGAAATTCAGGTTCGCTCTATCTGTATTGGCGGCAAATCCCTTGAGCTATTATCGCTCTCAAAGGGCGACAACCTTCCGGTTATTACCTCTCGGAATCTTCTTCCGAGCAAAGTAATGGTTTACAGGAGCACTCGCTCCTAACCCCATCACTCTCATGGCATCAGAGAAAAGTAAAGAGCTCCCTAAGTATCTCTTGGGGAGTTTTTCAATTCCTTTGGGAGTTATGCTAGGATTTTACGGTTGGAAATCCTAAGAACGGCTTATTTTTGTCAGCTGGATCACGGTTCATGGGGTAGCGTTGAGAAGGATCCCAAGGTGACGCTCTACTCCTCTATTGAAAATAGTCTGATAACAGTCGGAAGGAAGCGTAGGATTTGGTTGATAGCCATTGAGATATGCTAAACATGAAGTTGCTGAGGCTCAAGAGGTAGAAGTCTGACTGGCAAAGGTTGGAAGGCTATGATTCATCGCCCCTATGCTGTCTTCAGCGAGAGGATAGGTATCAAAAAGATCGTTTCCATACGTGTAGGCGTAGGGATAGTAGAGTTGTCAGGAAAGGATTTCGATTTGTATCCCCTCAGACTCGGAAAGTTTGAAGAAATCTGGATATTCCATCCTGATCGGAAAGACAGCATTCCTCTGCATTTGCTGTTCACGATCCATGCGGTAATCATCAAGGTTGATGATTCCTTCTTTAGAGAGGGGAATGATTTTGTCCTGATCAGATTTTGATCTCTTTTGTCGAGAGGTGGCTGAAATCGTCGTCGTATCGCTTCAGCGTTGTCGGTCCATTTTGTAAGCTTCGAGGCTTCCTGCGAGGTGCTTCGCCATTCCCATCCTTACGCCGTCAAAAGAATAGGTTGCGCCAAGGACAAGCAAGGTAATACTAAATAATCCTCGTGGAATAATGATCTTTCGTTTCATTGCGGTAAGTTATTCAGATAAAATATTGCTTGTGAACTGGCGTAGTTTATATAGTTTTTTTTGTTTTTCAAGGGGAAGTTGCTAGGAAAGTCTTCTTGACAAGGTAAAATTGTGGGAGAAAGGAGATAAAAATCTGATTTTTGACTTTTTTTGTAGAGAGAGCAGCGAGATTGCTTAATGGTATCAAAGATTACGCTCAGCATTCTCGCTCTGATAGGCTGGGATCTGGTGAAGCGAACAGGGGACGAAGGGAAGTTGCTCAAGCTAAGAGAGCTTTGGGTAATAAAACGCTCTGTCCTTCGGAGACCGCTCCCCCTTATGAGAGAAAAAAAGCCATGAGAGGTTGCTGTGGTCGTCTCTTTGGGAGGGGAAAGGGGGTAAAGCGTCCTTTCGGTTGCTATGACGGGTTGAGTGATGTCAGAAAGACCTCGTCTGGCATTGCTATCGTCAAAGCAACCCCAGAAAGACCTCCGCTGGCATCGCTATGACCAAAGTAAGCCCAGAAAGACCTCGTCTGGCATCGCTATGGCTACAGTGAGCCTAGAAAGAGTTCAACTCGATCTTGTTCCGATACCTTTGAAATGAAATCAACGCTATCGGGATGGAGCTTACTTGTCTGCAGCCTCTGTATGACTCTTTCCAACATTTCGTCCGAACGCCTGCCTATCGGGAAAGGTTGCTTTACCTTCGGGGAGATTGGCTCTGCTTTCAGCTTTAGCATCAGCTTGTTCAGGATGAGTGGCAGAGGAAATGGAGTGCCACGATGCGGTTGTTTGCGTTTCGCTTCGCTTGATTGAGGAGGTCCTGCGGTCGCTATGATGGGTTGTGGAGGACGAAAAAAGAGTCAGCATTTCGCCAACTCTTTGGAAGAGATTCAGTCTTTTATTCTGATAAAAGCTTCTTTCTCTGTGATTATACATGAGGGAAGCCTTAGAATTTAACCTTAGGAGCCTTCCTTTCTGAAACATCGTCTAACTCAAAACTTTTCTCAGTTTTGAAACCAAACATTCCTGCAATGAAGTTGCTTGGGAAGGTCTGAATAGCATTATTGTATTCCCTTGTAGCAGAGTTGAAGAATCTCCTTGCAGCAGCTAGCTTATTTTCAATATCTGAAAGCTCCGTCTGAAGCTCGAGAAAGTTTTCGTTTGCCTTGAGCTCAGGATAACTCTCAGCAAGTGCAAAAATAGATTTAAGAGCTCCTGTGAGCTGGTTATCAGCAGCAATTTTGCTGTCTACAGAACCTGCATGAGCGAAACTATTCCTTGCTTCAATAACTTTTTGAAAGGTTTCCTGTTCGTGCGTAGCGTAACCTTTAACCGTATTCACGAGATTTTCTACAAGATCAAATCTGAGTTTGAGCTGAACGTCGATATCCGCGAAAGCGTTCTCTCTATTATTTTTGAGCTTCACCAAGCGGTTATACAGGATTACAACGAGTATAACGATAATCCCGATAATTATCCAGGACGCATTTAGAGTAAATGATGTTGGCATTGTAAGCTAGGATAGAAAATAAAATTCTGATTTATCAATTTCAGGTAGTGTTATCATACGAATTTCTTATAAGTTGTCAAATGGAAGTTTTGATTGACTTTCTCTTGATTTCTCCTAAAAACTCGGTATATTTGCTTCCAAATAGATGGTTCATAACCTGATTTTATTTTCTTCTCCTTTCCCTATGCCAGAAAAGAAGACAACAGCTTCGGCTCCTAAAGCAAAAGCTCCCAAGACTCCAGCAAAGAAACCAACCACTTCGACCCCAAAAACTAGGACAAAGACTGCTTCTGCGACGAGCACTTCTGCGACAAAAGCCAAACCTCAATCTACTCCAAAGCCAAGGAAAACATCGGCTCCAAAAGTAGCAGTGAAGACTAAACTAGAACGCAGTTTCTCTGTAAAGCAATTGGAAGAGGTAAGACCGTTGGAAGCCACTCCTGAGAAGAAGAAAGTCCCTACATGGATTGTGGTAGTATTTATCTTTTCACTTGTATTTTTCTTTTTTGCACTTTACAAGGCGTTTATCTATGGGCAGGGCTATGATCTTTCCCCTCTTGATACCTTCACTCCCTCTCAGTGAAAGGATGCCGAAATCTCTATCAGCAACTCCTCTACTGATGAAACTACTGGGGTTTTGGTTCGTTCTGGAACATGAGTAGAAGCAAACCTTGCAGATGATCCGCAGTTGATTCAGAATTTTTATAGCTACCTCACGAATAATCAAATCACTGAAATGAACGATCTCGTTGACCGTCCGCTCAAAAATTCGACCGCTTGGCTCAACCATTGGAACCAGAAAAATATTCAGATTTTTACTAAACATTTGGCTGACACAGTGCTCCTCCAAGATATCTTCCTCATCCCTGGATCTATCAACACTCAGAAACAAACAAGACAATATTCTTATACTCTCAAATATACTATCCAACCCGATCATCACTTTGAGGAAGAATGGCAGGTAACTCTTGCTACTCGTGATGGGAAGACGTTAATCTCCGAGATTATGTGTAAGACTGAAGGCTGCTCTCGTTCTCCTTTCTTTTGGCCTCAAAACTACAACTTGAAATAACATTATTGGCGATGAAAAGTATTGAAATAGGCTTTAAAATCAGATTTTTTCTTTTGGGATGGTTACTTAGCATCGTAGTCATCCCTTTTTAGTATGATAGCCGTCTTTGAAGAAATGGTAGCACACAAACCAACGCTTTTTAATGAAGAACAACTGAATACCCGAGCAAAGCTTCACAAGCTCCAACCGTTCAAGGTCAAGCAAATTCTTTTTGAACTCTATAAGAACCAGAATACTGAACGAGATGCGATGACTACCCTTTCAAAGGAACTCAGGTCGCAGCTTCAGTCAGACTTTGATCTCTTGGCGATAGAACTCGTAGAAATGGTAGAAGCTGCAGATACTACGAAATTTGCGTTCAAAACGAGAGATGGGCACCTGATTGAGGCAATCCTTATGTATCACTGGCAGCATGAAAAATATGTTCAGAATCAAACTCCTAAACTTAACCGTATCACACTTTGTATTTCCTCTCAGGTCGGCTGTGCAATGCAGTGTCTTTTTTGTGTGACTGGGAAATTAGGGTTCAGGAAGAATTTGACCTGGGAAGAGATGATTTCTCAGGTGCTCTTTGCTAATAGCTATATCAAGAAGAGGTTCTGAAAAAAAGAAGACGGGACAAACTATAGCGTGAGAAACATTGTTTTTATGGGGATGGGAGAACCGCTTCTCAATTATGATCATACGAAGATGGCGATCAAGCTTATGCTCCAGCAAGATAGGCTTTCTCTAGGAAGGAGACATGTTACGATTTCTACGTGCTGAATTATTCCGGGAATTGAGCAGCTTATCAAAGATCAGATTGATGTGAAGCTGGCAATTTCCCTCCATGCTCCTAATCAAGAGTTAAGAAATCAGATTATGCCTATCGGGAAGTCTTATCCTTTAGATAAGCTTATGACAACGATTGATCAGTATGTTGAAGCAACGAACAATAGGATTTTCTATGAATATATTATGATTAAAGATCTAACGGATACTCCTGCATTAGCCCACGAACTCGTCCAGCTTCTCAAAGGCAGACTCGCTCATATTAACCTCATCCCGTATAATGAAAATCCTGCTATTCATCTCCAAGAAAGTAGTAAGCTAGCTATCGCAACCTTCAAGCAGATTCTCGAAAGCTGAGGATTAACAGTGACGGTGCGTGATAGCATGGGAAGAGAAGCAAAAGGAGCTTGTGGACAGCTCGGCTATGAGAAAGTGAAGGGACAGCAGTTAGGCTTTTAGACAGCATTTGCTCCTGTATTAAAAAAATTTTTATTTAAATAAAAATCTGACCCTTGAATAAAGATCAGATTTTTTTTATTATTTTCTACTGGCTTCACAAGTTATTGGGTTTCCTCCAGAAGGAGACACGCATGTCCAAGTAACTTTTGGACCTCAGCTGATAGGAAATCCTGGGGCTGTACCTGTTAAGGCAGATCCTTCTCCACAAAACTGGGAGATTGGTCTTCGTGTAGTTTCTGCTTTTTCATATTTTCTTGCTTTATCTCCACATTTTGCAGCTCATTTACATGCATTTGCTCAATTTTTACAGACATCATTACAAAGTGTATTATTAGAGACAGATTTTAGTCCATCACTTCACTTCATACAGAGACAAGAAAGGGTATTCCCTTGGCACGCCCTTCTGAAAATTGTTCCTTCAGGGAAATTATTAGGGTTTGTCTCACTTCACGCTCCCACTCTCAGGGCTCCTCCAATTGTAAGCTGAATATCTTTACTTTTTGCATCACTAGTTCCGACAATCATTCCGTAATCAGCATTCACAAGGAAGGTCCACTGTTTTTTTCAGGTAAGCGTCTCTGGCTTTACATTAAATGCAAAGATTCCTCAAGAAGCAATCTCTACATTTGATCCCAAGACGAAAGATTCTGAGGCTCCAGGTTGGATTTTAACATTAGAGCCTCCATAAGCGGCAGAGTTTTCTGCATTAATAATAACATTTGTCGCAGCCACTGCAACCGCACCAGATGCCTCGATTCCCAAGGTTGCTTTGGTCGAAGAAAGGATTACTCCGTTTGGAGCCTGATTGTTATTTTCGCTTCCTCAGAAGATAACGGCATTATTGGTATCTTTTTGTTGTCCGCCAGTAGATGCTGTATTATTCTTTCCCCCAACGAGGAAAGATCATTCTCCAGCGGTATTTTTATTTCCTACCCCTGCAATAATAGAACTATTCTCATCGGTAGTATTGTTGAGTTTTTCCATCTTATCTACTTTCCCTCACTGCCCTAAAACTACAGAGGCAATTCCGAGTGTCGTCCCTCAGGTATTCATCAGTCACACTTTACTCACCTTCCCCTCCTTTTTTTGATTGAGGTGCACCTCCTTGATATGCTGAATAGCTGTAAATCTATTCGTGCCAATCGTAATTGGAGTGTTTGATTGAGCAAGGACTCAAGAAACTGCCAACGTGAGCGTCAACGCTCCTCAAAGCAAGAGGAGTTTGTTTGTAGCAAGATTTTTCATCATATTCACAGATTAAAGAATAAACAATGAACTTTCACTGCCTCAAGTATACAGATATTTTCAGATAATGCAATTTTTTTAAAAGAAAATAGCGTCCCTTGATAAAGAAACGCTCTGATCCAAAGTTTTGACGCAGGAATTAAAAGCTTCACTAGAAAGAAATCTGATGCTTTTCATCAGGAGACAACAAATTATTTTGCTCCCGTTAGGTATTTCCCTTTGAACTGCTGATAAACGAAGTCGAGCTCTTTGTCGAGCTTTGCGAGTTCCGCCTGTAGCTTCATAGCATTTGCCGAAATTTGCTGACTATCCTTTCTATAAAACTCCGTTGCATCTCCAGAAAGATTCACCAAGAGCCTTACCGTCGGATATTCATGCTGCATAAAAGCGGTTCTTACCCCGGAAATATAAGCTACAATCCCTGCTTTGAAGCCCTCATCAGGAACTCCAGGATAGGTTTCGGCGTCAGTAATGAGTTGCTGCAGTTTTTGAATGGTAGCTTGGTAGGTTTCTTCTAAGTCAATCTTGCTATATTCTTTATTTAAGATCTGATAATAATTTTCGAGGGTCTCAACGGACTGTTCCTGGAAAGTAAGGAGCTGATTATTATATTTTTGGATATCGACCTGAGGCTTACGAAAGGAGCACCCTGCAACAAGAAGAACAAAGAGAAGAAATCAGATTTTTTTCATTTTGTAAGGCGTAAGAATAAAGGACGATACGGTTCCTGCTTCTGGGAAGGATTCCTGAATAGCTTCCTGAGTTCTTATTCAAATCTCAATGCATCGATAGGCTTGAGTTTCGCAGCTTTTCTCGCAGGGAGGATTCAGAACACAATTCCTGTGAGTGTCGTAATAAGAAAAGTTGTCATCATAATACTTTGATCAATTTCTGCACTCAATCCTAATCCGCTATTTGGGTCTCAGAGGAGCTTATTCACAAAATACACGATACCATAGCTCAATAAAAAGGCTATAAATCCTCCTATCAAGGTAATAACCACAGACTCTACAAGAAACTGGATAATAATATCTTTATTCAAAGCCCCGATTGCCTTTCTGATTCAGATTTCTCTTGTTCTTTCTGTTACGGAAACGATCATAATATTCATCACCCCAATTCCTCCAACAAGCAGAGAAATCGCTCCCATTACCAAAAGGAAGAGCTTCATCCCCTGAAGAGATTGTTTCATTTGATCAACCATCTCTGCGTAGCTGTGGACTTTGAATCCCGCATCGCTTATTGAGGTTTTATTGTATTTTTTGAGGAGAAGATAGCTGATCCTCTTCTGCCAGAGTTTGTTATCTCCATCAGGTGGGAGGAAAACATCAAAAGAATCAATAGTATTTTTGTGGAGAATCCTTTCTGTAACTGTGGTATCCGGAAAATAAGCTTGAGCTCATCGGTCATACTCTTGTTTCGGTAAAACTCCTACAATAGTAAACTCTTTACCGTTATAGATGAATTTTTTCCCTATTGCGCTTCCATTTTTGAAGAACTTGCTCTTGAACGTTTCGTTAACCACGGCAACGAAACTCTTGCTATCGTAATGCTCCTTGGAAAAGAGAGTTCCTTCAGCTGCTTTCTTTTCATTGAGAGTAAACCGAAGTTCTGGAACACCAACCCCCATCACCATATCTGATTTTTGTTTTATTTTTACCTGTCAACCAGGGGTTTCTATTTGATAGGTAATTTTTCAGCTTAGGTCTGGGAAATATTTTTCTATATAGTCGATGATCGTTTTATCAAATTTTACTGCCTGGATGTAGCCTCACGGTTTATCTTCTTTGTCATCTCGGGCATTGTAGCCTCCATCAGAAGAGACTGATATTTTATTTTTTGCGAAATCCCCCATCGTTTTGGTGATAGATTTCTCTGTTCATTGCCCGATCGCCATCATCACCACAACGGAAGCCATTCCGATGATAATCCCGAGCGTACTCAAGCCAGATCTCATTTTGTTGGCCATAATGGTCTTTCGAGCATCTACAAAGTAGGAAAAGAACCTCATATTTTCTCGCTAATATCTAAGGTAAAAGTTAATTTTCGACAAAAAGTCAGTCTTTGATACGGATCGTTTTATTTGCATAGCTCGCAACATTTGCGTCATGGGTAATCAGAAGAATTGTTCTTCCCTGTTCAGCATTTAATTTTTTAAAAAGCTCCATTACCTCTATTCCTGTCTTGGAATCCAAAGCTCCTGTTGGCTCATCTGCGAGCAAAAGTCCTGAATCTGTCACTAATGCCCTTGCAATACACACCCTCTGCTGTTGACCTCCAGAAAGTTGGTTTGGAAGAGCTTTTTCTCTCCCCTCAAGTCCTACGAGTTTAAGTGCAGCATAGGCCCTCTCCTTAGCTTCTCCTGAAGGAATTCCCATATACGAAAGTGGAAGAACCACCTGCTCTCGTGCTGACATTCTTTTGAGCAGATTATAGCCCTGAAAGATGAATCCGATTGTTGTTCTGCGGATCATCGATTGCTGGTCTGGAGTCAGATTTTTTACCTCTTTCTTACGGAGAAAATAAGAGCCAGAAGTCTGAACATCAAGAAGTCCGACGATATTCATCAAGGTGGATTTCCCGCACCCTGACGGTCACATAATGGAAATAAAGTCTCCATCATGAATATCAAGATCAACTCCTCTTAGAGCATATACTGGCTCTGTCATCTTTCCTCCATAGGTTTTTGTGATTCCCTTAAGTGAAATAAAATCTGATGTTGCTTCTACCATTATGATAATCACAAAAGAATAAAAGTTTTTGGTAATTTTTACATTGAATATTCATAACTCATACTACCATCCGCCTCTGTCGTTATATTTCCATCTTTTGGTCAGCCCATACTGCCAACACCCACATTCCCTGCAGAAATATCGTTCATATAGAATCCCTTTATCGTATCACCAAGCTTAAGACCAGAAAGAACTACCGTGTTCATTTCATCAGCAATTCCTACTTCTACGACCTGATCAACTCGCTGTTCTCCCTTTTTGAGACGCACGATTTTTTCTCAGAGTTCGTTGTCTGCAAGTGCAGGATTAGGAACGATTAGCTTTCCTTTTGCTTCTTCAAGGGTTACCTTTACTGTCGCACTCATTCCTCCTAGAATCTTTTCCTCAGGATTATTTTTTTGGAAAACTACTTTAGCTTTGTAGGAATTTCCCTCTGGCATCGTATCAATTTCAGAGAAAACTCATGAATATACTTGGTCTGGGAAAGCACCAACAGAAACCTGGACTTTCATTCCTTGCTGGATCTTTACGATATCAAGCTGATCCACCTCAATTTTTACCTCAAGAAGATCTGGTGTCTCTACATAGATGTATTTTTGATTATCATTTGTAGAGTTTGTATCAATACTGTCTCCAACCTGCATATCAAGTTTGGTTACTCTTCCATCGAAATTTGCAATAATTTTGTATTCATCATACTGCTTCATCAATGTTGTCAGTTCAAACTGCGCATTGTCAATTTCATTCTGGACTCTCTGAATTTCGTCTTTTTCTGTGCTTCACTCTCTTGCCTTTTCTAAATCAACCTTTGCGTCTTTGATCTTTTTTTCAAGTTCTGCAGTAGAAACCAGCTGCTGTTTCTGATTTACTTCTAGTTCTAGTTTTTTCTTTTGGAGAGCAAGTTTGAGATTTTCTAGCTCGATCTTTAGTTTTTCAACCTTGTCTGCTGCATCTTCAATAGTCGTGTCTGAATCTTTGAGTCAAGCCAAGGTTTCAACTGCTGAAGTATATTTATCTATGTAGTCATATCCGAGTTTTTCCAAAGGAGCTCAAAAAGAATTCATATAGCCATCTATCTGTGATTTTGATAGAGAGCCTTCTGATTCAATACTTGCATTAAACATCTCATAGTTTATTTTTCATCGATTCCTTAGATCAGCACCTAACTCTTTGAAAACAGTATATCCTGAAAGAATCTTGTTTTCAGTCAGCTGACCAACCGGAATCTTTGAAAATTCAGTATAAAGATCATTAAGTCTTGCCTTATATGCATAGACTTTTCGGAACTGCTCTTTTGACTGAGCAATTAATGCCGTATTTTTTGCTCCAATATAGGTATTCATTTCTTCTCCTTTGTATTGGTCAGTTAGTTTCATCACCGCATCAAAATTATCTAGCGTAGATTGCAAAGCATTTGCTTCCTCACGAAATGCTCTCACCAATTTTTCCATAGAAGTGTTTCTGGTTCTTACGTTCTTGGAAACTGCTAAGTCTGCATTCGTTGCTCCTGCCTTTCCTGATAGGAGCTGAGCATAATCAGTCTGGGCTTCTTTGAGGTCTTTCTCCTTTTCTCTAATTTGCCTTTCTGCTTCTTCAAGATCAAATTTTTTGGTCTGATCATCAAGCTGCTGTTCACTTGGCAAGGTTTGTTTTTGCATGACCAAAAGATCATAGGCTGCCTGTGCTTTAAGGAGTTCAAGCTTTTGATCTGATTTATCGAGAATTTTCTTGAGCTCAAGCTGCTTATTTTTTAGGTCCTTTTTTGCTGATTCAACCTTTCTATCAAGATCGTCTGTCGTAATTGTCGCTAGAACATCTCATTTTTTCACATTAGCTCAGATTTTGGTCCTTACGCTTGTTACTCTTCCTTTATTGACAAATGTCAATTTTTGAGCGTTTGCAAATTGGGTCGTTCCCTGAAGGTTTAGCGTTTTTTCTACGTCTCAGGTGATTACTTCATAATCTGTACTCACAAAAGGCTGGTTTCCTCCTTGTTTTCCGTAAAAATGCTTGTATCCATAGATGGCTCATCCGATCAATACCACCAAAAAGAAGGCAACCCATCGCTTATTCCTCCATAGCATAGCAAAAAATCCTTTAAAAACACTCATCAGTTCAAAATGAAACAAAATAAAAGATCTGATTTTTTTACTTTTTATTATCGTAAGAAAAACATCTAACGTGACATTTTATTTTTTCAGAAATCAAATACAAGAAAAAAGTGTTTGTTGCTTGACAATTTCCCTTAAATTAATAGAATTATGGTGTTTATTTTATCAGGAAGATAATGATTATTACACTCTGAGGAAAAGCCGGAAGCGGGAAAGGAACTGTTTCCAAACTTTTAGCACAAGAACTAGACTATGAAATCATCTCTATCTGAACACTCAAAAGAAAGATCGCAGAAGAAATGGGATTAAGCATTTCAGAGTTTAATCTCTTGTGAGAAAAGGCTGAGAACCAGAGAGAATTTGACCTAAAATATGAAGAATATCAAAAAAGTCTTCCACTTGAGAGTAGAATTATTCTAGAGAGTAGACTTGGATTCCTCTGTCAACCAAAGGCTTTTAAGGTTTTTTTGGATGTTCGTGATGAGGTCGCAAGTGAAAGAATCCGCAAAGATCAGAGAACTACCGATCAATTCTCTTCAAAAGATGAGGCTTTATCTGCGACAAAAAAAAGGAATGCTGACGATAGAGCCAGATTCCTTTCTCTTTATGAAGTTGATCTCTGGGATGATAGGAATTATGATCTCAAAATAGATACAAGCGACCTCTCTGCTGAACAAGTTTGCTCTAGGATTATCGCCAGATTTAGCGAAATAGTCCCTATTAAATAAGGTCCCAGCAGTAAGCTGTATATCAGCAAAAGTCTGATTAAAAATACTGACTCTTAGCCTAATTCTGAGAGTAGGATTTGGGTGATTGCTTGAGGATTCGCTTTTCCCTGCGTTTTCCTCATTACTTGCCCTACAAAAAATCCTATAACACTGGTTTTCCCTGCTTTATACTGATCAAGGATTGCTGGATTCTCGTCTATAACCTCTTTACAAAAAGCTCTTAAATCAGATTCTGAGATCTCTGGACTATCAAAGCCTTTTTCCTTGATGATATCAGATGCTGAAGCTCCCGTTTGGAGCATTTCTTCCATGATGAGTTTGAGTTGATTGTCAATCAGTTTTCCTTCTTTTGCAATGTTGAAAAACTCAATCAACTGCTCTTGGTCGATAGGAAGCTCGGTAATATTCACAAAATGTGCCGTCATATAGCCGCTTATCTGCCCAGCAATCCATTTCGCTACGAGTTTCGGTTCAAACCCTGCATGTATCAGCTGCTTGAAAAAAGTAAGCGTTGGATATTCACTAATTAAAGCATTGATAAACTCCTTGTGAAATCAGTATTCTGACTTCATTTTTTTAATTTCTTGAAAAGGGATAATCAACTCAGCTTCCTCCACCTTTTTAAGAAGCTGCTCATCAAGATAAAGCGGAAGCAAATCTGGCTCTGGGAAATATCTGTAATCAAGCGCGTCTTCTTTACTTCTCATCGCAAAAGATTGTCACTTAAGATCATCTCGTCTCCTCGTCTGCTGCTGAATTTCTTCTCCTGTTTCTCGGCATTTTGCCTGTCTTATCACTTCAGCATCTATTGCTCTTTTTATAGCGGAAAAGGAGTTGATATTTTTGAGTTCTACCCTCGTTCCTAGAGGATCAGATTTTTCTTTTCTAATGGAAACATTGACATCGACTCTCATTTGCCCCCTTTCCATATCTGCATCTGAAAGGTTATTTCGTTTTGCAATTCTTTGGATTTCTTTGGCAAACTCTACAGCTTGTTCTGCGCTGGTAAAATCAGGTCCCGTCACGATTTCAATCAACGGAGTCCCGGCACGGTTAAAGTCTAGCAACATTGCTCCTCCCTGATGAAAAGCCTTCGCAGTATCACATTCTAGATGGGCGTCTGAGATTCTTACCTGTTTCTCCTCTTCATAATTTGCAAAGAAAAAAGAAAGTGTTCCTTTTACGTTAATTGGCGTATAGAGCTGAGTAATTTGGTATCCCATTGGCAAATCTGGATAGAAATAGGATTTTCTATCAAAATATGAAGGATTATTGAATTGGCATTGCAGAGCCTTTCCTAGCAGAAGTGCCTTCTGTACGACCTCTGCACTGAGCTGAGGCAATGCTCATGGCTGTCAAGTACATACCGGACAAATAGTGGTGTTGGGCTGGTTATCTTGAAGGGCTTGTTCATTTTTACATTTACAGAAAATTTTGGTCTGACTTGCGAGCTTTAAGTGAATCTCTAGTCCAATAGTAATGAGCGGTTCTTGCATATTTAGGAGGAAAGAAATGAAAACTGACTGAGAGTATAGCAATTTAAATACCGAAAACAAAAGATTTTTTAAAAAATCTGGGATATAAGAAGACACTGACTTGAAAATGCTCTCCACAACAGTATAGATAGAGTGTCTATGGTATAAATTTTTTATCATCTCGTATATTTTGAATGCATATTCATCTTGTCAGCATTTTCCCAGAAATCTTTGAAAGTTTCCTTGGGACTTCTCTTATCAAAAAAGCACAGGAAAAGTGAGTACTCGCCTTCTCGTGCTATAACCCGAGGGAATTTTCTACGGATCCTCACCAACAGATTGATGATTCAGTCTATTGAGGTTGAGATGGAATGCTGATCAAAGCACAACCCATTATTGATACTGTAGAATTTGTGATCAAGGATAACCAGATAAAAAAATCAGATTTTTCTATTCTTTTTCCAACGCCAACCGAGCAGCTCTTTAACCAAAAAATCGCTCATGGACTCAGTAAAAAAGAGCATCTCATTTTCATCTGCTGAAGATATGAATGAATTGACTATAGAGCAGAACAATACTTTTGAGATCGCTATCCTGACGCCTTTAGAAAGTATTCCATTGGTCCTTTCATAACCCTATGAGGAGAGGGACCAAGTTTGCTCATGATTGAGGCTATTACTAGACTAATTCCTGGAGTTATAAAAGAAAAAGGAAGCTGGATTCATGAGAGCTATTCTCCTAGAGATAATATGAGCAATATTGAAGCTCCCAATTATACAAGACCGATTGAAGTCTATGGGATGAAGGTCCCAGAAGTCTTACTCAATGGCGATGAGGAGGAAATCGCTCAGTGGAGAGAAGAACATAGTATTAACCTCTAATTTGCTTTTATTTTTTCTTCCTTGATTATGGATTCCCTAAAAACAAAATGATACAGGGGAGAGTGAGTCGCTCTTGATTGGTATATTCAAAGGTGATATTGATTCCTTGATAAAAATTTTACGATTCGCGGAGGAGAGATTGATCTAATTGTAGAAAATGAGAGCTTTATCGTATTTATTGAGGTGAAGGTTGTAGATAGTATAGAAGAATGGTCTGGATATCTCTCTGCAAGAAAGCTTCAGGCGATAGAGAGGAGCATAGAAGAATATTGCTACCGTAAATGAATAGAAAAAGAAATCAGACTAGATCTTGTATTTGTCAAAAATCAACAAGTAATTGAAGTTTTCGAAAATATTAGCAATAGTTAGAGGCCGAGATTAGGACTGAAAAGTGGGCGAGGATACCTATACCATGTAGGTATTTTTTGAATCAAAAAAAATCTCAGCTTAAAGCTGAAATCCTTAATGGAGCGGGTGACGGGACTCGAACCCGCAACAGTCTGCTTGGAAGGCAGGCACTCTAGCCAATTGAGTTACACCCGCAAATACAATTTGCAATGTAGAATATATTTTTCTTTACCCTGATTTCAAGTCTTTTTCTATGGGCCTAGCTGGACTTGAACCAGCGACCAAAAGATTATGAGTCTTCTGCTCTAACCAACTGAGCTATAGGCCCATACCCAAGGCCCAAAAAAGAAATGAAATTAGGAATCTTAATGGTGGGTGATATAGGACTCGAACCTATGACCCCCTGCTTGTAAGGCAGATGCTCTAGCCAACTGAGCTAATCACCCATTAAATCGGTCTTACGGGGAATCGAACCCCGATTAGAAGATTGAAAGTCTCCTGTCCTAGCCGTTAGACGATAAGACCATAAAAGTTAGAGTGGCGGGATGGAAGGGACTCGAACCCTCGACCTCCTGCGTGACAGGCAGGCGTTCTAACCAGCTGAACTACCACCCCAGCTTGGAACAACAAATCATCTAACTTGATTAATGGAGCCAGCTGTCGGAATTGAACCGACAACCTACCGCTTACAAGGCGGTTGCTCTACCTATTGAGCTAAGCTGGCATTTAAAGTCCTCTCCGGACTATTTCTTGAGAGTATCAAAAGCTTTTGAAACTCTAGCCTTTTTTCTAGCGGCGTTCCTCTTCTTAAGGATTCCCACCTTAGCGGCTTTATCAATAGCTTTGTATACTTTACTCAAATCCTCAACAGTCAAAGATTCTTTTGCCTCAACTCCTTTAAGGAATTTTTTGATTGCCATCCTCATCCTCAACTTGAAATCGTAATTCCTCTTTGCAAGCGTTTGAGATCTATTCATAGCTTTAACCGCGGACTTCTTGATTGGCATTAAAAAAACACATAGCAAATATAAAGCACAACTCTCCGAGGGTGGGGATCGAACCCACGACCAAATGGTTAACAGCCATCTGCTCTACCGCTGAGCTACCTCGGATTAATTGACGAAAGACGTTATACAAAATTCGGACCCATTTTCAAGAGATTTTTCACTTTTTGTGGAAGATAATTTAATCGATGATCCACAATCCCAATTCCCACAATAGGGAATCAGACTTTTATCTTTTTAATCAAACCAACTACCGGGAACAGGACTTGAACCTGCACACCGTAAGGCACTTGGTTCTAAGCCAAGCGTGTCTACCAGTTCCACCATCCCGGCAGAAGTTGATTTAATACTCGGGAGATAGGACTCGAACCTATAACCCCCAGATTCAGAGTCTGATGCTCTACCAATTAAGCTACTCCCGATTATTTTGGTTGCGGAGGATGGAATTGAACCACCTATCTTCGGGTTATGAGCCCGACGAGATACCGTTTCTCTACTCCGCATTAGCAAATCACAATCTCTTTTATCACACCTGGGGAACCAGGATTCGAACCTGGACAAACGGAGTCAAAGTCCGGTGTCCTACCTTTAGACGATTCCCCAATCAATTCTGATTTCTAGGAGGGTTTATATCTTTTTGGCGGAGGATTTCAAGAGAAAGTGTAAGAAAAATGAATGACAAAAAAGAATAACAATAAATATTCAGAATTCTGACTTTTGCTGCTGATTTTTTAAATAAAAAAAGTAGACTGATTCCCAGCCTACTTTCAGATTTTTTAGCTTTTTGTTTTTGTAGTGATATTATTATTTTCTATAGACTTCAAGTGCTTTATTGAACTCTTCTGCACCTGGAGGAAGAGCACCTCGCAAACTTGGGTTGTATTCTACGAGAACTGACGCTCCATTCACAATAATATCCTTTTTCTTTTCTGATCCTTTGCTAGAATCTGCACTAAACCATGTATAGAGTTCAGATCTTCTCTTCTCAACAACATCTGAAAGGTCTCCGATTGCAACTCCCTTGATATGAAGGTTTCCATAGTTACATCGTTCACCTTTTCTGAGGTTTCCAAAGGTATTTTTGAGATCCTGATCATTAGATGATTTAAATCCTTGTCCTGCATAGAAGATTCCTTGCACCATCTGTCCTGCGTGTCCATAGGTCTTGCGATCTCCATTACAAGAACCACCTGCATCAAAAGTAATGGTTCCCTCCGTCATAATCATTGCATTTGTAGGAAGATTACCTTTGATCGTAAGGTTTTGTCCCTTTGAAGCGATCAGTGTAAATGGTTTATTGGTTATTTTTGCACCAAGTAAGCTTGTAAGCTCTTCAGTTCCATCATAAAAGTAGATTGACTTACCTGGAACCTTTGATAGTCCGTTATTTCCAAGAGACTTTGCTAACTTTTGGTACTTATTTTTGAACTGAGTGAAGAGATTCTTCATATTCTTTGGTGTTTCATACGCCTTATTCGAAATCTCAGTCGTCTTGAAGAACTCACCCATAAAGTTAGTTCCGTTTTTCAACTGGTATTTCGTAAGGTCTGTCGTAGCTTTATTTCCAATTCCATATGGACTCTTTTGGATAAGATAAGGCTCTGTTACTGCGAAGTCTACTTCACAGATGATTTCCCCTTCCCATCTCAAATTACCAGCGGTTCCTTTACCATCTTTGTCAGTAGTACATCTCTTGTATCCTACATCTTCTAAGGCAAGCTTGTATTCTCCAAGGAGACCACCGTTTGGCATCCACGTAAGTGTAGTATTCTTCTTATCAGTTATTGAGGTAGTTTTTCCAAATTCTTTGATCAGCAACTTGGAAGATAATGGAAGTAAGATTGGATAATCTTCCTGATTATTGAGTGAATCAAAGACATTCTTTACGAGTCCTTTATTAACGAGCTCACTTCCTCCGAACCAACCTTGCATATTCTGGCTTACTCGGTCTTCTACTGCTCAGTATTGATTTCCTAATGCAGTATCCCATCCGTTAATATCTACACAATCTGTAGTAAACTCATATACCTTTCTACCATTTCCTTTTCCTGGTGCATAGACTGCAAACTTACATTGCATTGAGCTAAGTTCGATCTTGCTATTTGCATCACGATCTGAACATGAGTAAGCAAAATTTGTCTTCAACCAATTCTGCTTTTCTGAGACAGTTTTGAGTCCAAGCTGAGCTTGCATATTCCAGTAGAATGGAAGCGCTTCTCCCTTCATAATGGAAATTGAACCATTATTAATATTGAAACACTGAGGAGCAATTTTCTGTCCCTGACTTTCTAGTCTACACGTATTGGTACAAGTGTATCCTGCATATCTAGTATCATACTCGTCCGTATTCCTGAAGAGTCTTCCATCAGATCCGATGGTTCCATGTCCTCCAAGATCACAGACTTCGTTTCCGTCTTTTCTACCATTTCCACAGGATGGAGCATATCGCCCTCCTCATCCTCAACCTCCTCATCCTCGACTACCACCTCATCCTCAACCTCCTCATCCTGAGGTAGTCTTGTAGACTCTGGCTGGGTCTTCATCATGTCCTTCTCCACCATTTGGTTTTGGATAATACACATGACCGATATTGTTTTGGTCACCTTTCTTCTGAGTCTTGATGATAATAGTGAGGGAATCACCTGACATAAAGTACCCTGTTCCCATATCTACCTTCCATGCAATATCTGCAGGCTGAGTTCCTGAAACTGTAGGAATAATCTTATATACTGGCTTACTTGGATTATTTGGTCCAAAAGTAATCACTCCAGTAAAAGCATTATCTGCTAGAACCCAAGAACCTGTATCAAAAGTCAGATTTCCTTCTATTCTATCTGTTACCCTGAATCCAGTCAAAGGTGCAGTTGCATTGGATATTACAAGCTTAAAGTAAGCTGGCCCATTGTCCATATTAAGCTGATTATCTTCTCGTGAGCCACCTACTGAGGTGCTGACGTATTTTTTGATCTTAAGCTTTACATCTTCTAGCTTTGGAGTAACGTCCTCTGGATCACACTTTGGATCCTTTGGATCATCAGGGTTGAAAACACATGCCATATTGGTGTATTTCGTCCTTGAGGATTCTACCAATCTTGCCTTAAGTTTTAATAGGAAAGAATCTGATTTTTTGAATCCTTGTGGGAATTTCAGTTCCCAATTGAGTGTTGCTTCTCCTGTTCCATTTACACCCGTAGCAAAGCTTACGAGCTCTGTTGTAGTAGTTTGTCCTGTAAGGAACTGAGTATTCGGAACGAATACAAGTCCTTTTGGCAAGGTATCTACGACCTTCAATCAAGTAAATGTTCCTTCAGTATTTGAAACCTTAATCGTAAACTCTACGATCTCTCCGCTGAAAACCTGTGGTTTATCAACAGTTTTTTCGATATGAAGAGCTCTTGGAGCAGGCTGATACGGTTCAGTATCATAATCCCAAAGATAGTTATTTTCATAGATTCCTACTCGGTTTGTCCTATTGTCTGGGTTAGTATTAAGCACTCTACCTGTCATAAGGAGCACTCCCTGTTGCCCTGAACTGAGGCTAAATCCTGAATACTCTAGGACTTGTACTCCTCCACTCAGATAAACTCCGTTTGTATAAGAAGGCACTCCTTGGATTGCACTACTTACGTATTCAAGGTTTAGAGGCAGAATATCTCTAATCTTTACGTTATTAATAGTCTGACTTCCAGTATTTCCAAATGGCATTCTGAAGCCAACCAATTCTCCCACTTTGTATTTATGATTTTTATCTCCAATGAGATCTTTTTCGATATTTGGGCGGCTCGGCTTTACTGCACTACATTGCACAGGCGTGGATCCTGCTTCTCCATTACATGTCCAAGTCCATTTTTGAGTAGCTTGGTCATAGTTAAAGTTTCCGTAGGTTCCTGTAGTACACAAATGGTTTCCTCCGGTCAGATTTTCTACTGTTTGTCCGTTATATTGACTATTACATACTCCCTGAGTAAGCGTTTTTCTTACTGCACTACACTCTACTGGAGTTGTTCAGTTCATTCCGTCACATTTCCAAGTCCATCTATTGGTTGCAGAGTTGAACTGGAATTGTGTCATTGTTCCTGTAGTACACAAGTGATTTCCTCCGGTCAGATTTTCTACTGTTTGTCCGTTGTATTGACTATTACACTGTCCTGGGATTTGAGGAATGTCCTTGGGTTTACAGTTTTCATCACATCCACCTTTTCCCCAACCGGTTTTACTAGGATCATTTGGATCACAAGTTTCTCCATATTCAGGATCAAGAATTCCGTCTCCACATCGAGTAATTTCGTAATATTTACACTCTTTTTCTGCAGACCAGGCTCCATTTTGACTATCTGAATACCAAATAGTATACTCTAGATACAGATTATTTTTGTCTCTTTTTATAGGCTTTTCTCTCACGTAATATGGAGAAGTAGCAGTCAAGATTTTTATGGTTTGATTTGGATTAAGGATCATATTCTGATTTTTAATCTCTTTCGTCCATTCAAAGCTTGGGAATGGATCTGCTCCATTAAAATCAAGCTGTTCCCTATGATCTACCTTGAAATCTTTTAAATATCGACTTTTTGTTGGTGTCCCGGTTATTTGATCATCAAAGACGTATCCACGACCATGTCTTAACTTGCCATAGAAAGTAGCATCACAGTCAGCCTTTGGAATTATGGTATTGATTGGCTTACAGGAAGCATCACAACCTCCATTTCCCCAACCAGTTTTATTTGGATCTTTTGGATCACATTGCTCTGAACCGTTTTTGGCTCCATCGCCACATCGCATTTCCTTTGCGCTACAACTGACGTTGGATGAGGCATTATTACATGTCCATGAATAATTTCCATTTTGATCTGGTCCTACAAAAGATGTCACCTTTCCCTTGCTACAAAGTGCCATATCGCTATTAAGAGCAGCTACTGGATGTTTCTCATTATAGATAGTTTTTCCATTGTAATCACTATTACATTTTGGCTGTTCTACCACGGTATTGATCGGCTTACATGAAGCATCACAACCTCCATTTCCCCAACCAGTTTTATTTGGGTCTTTTGGATCACAGATCTCTCCTCTATCTGTATCTACTATTCCATCTCCACATCGGGTAATTTCGTAGTATTTACACTCTACATGAGAGGTCCTAGGAGCCTTCTTGTCCGGAGACTTGGAGTAAGTAACGGTGTACTTTACCAATAGGTTCTTAGGATCTCTTTTTTGAGGGACATCTATAACAGGATATTGAGTCCTAGCCTCTAGGAACTTAATTGATTGCCCTGGCTTAACTTCATAGTTCTGTCCTGTTATTGCAGCGGTCCATTTGAACTTTGGGAAGTCGGGAGACTTATTATAGTCCTGCTGCTCTACAGAATTTACTTTATAATTCCATAGGTAATAGCTAGAATCTGGAGTCCCATTGAAAGTATCATGAAAAGTATAACTATTTCACTGTCTCAGCTTTCAATAGAAAACCTGATCACAAACATCGTTGGCATAGGTTGTCATTCCTAATACGGTCATTCCTATCATAACCATGGAAAGTAACATTTTTTTCATCATCATTATTGATACTTATTAAAAGCTTATTCTTCTTCTCTTCCTCCTCCCTGTTCCGTCTTATCTCCATCTCCTCCCACACTTGGCTCGACAGGAGCTGGAGTCTCTTTTATCTCTGGTGTAACTGGAGTCTCAGAGACTGCTGGAGTATTTGGTACCTGAGATTCCTTCGGTGTTTCACTAGGAGTAGTTAATGGATGTTCATCCTCACCTGGAGTTGTGTCTGGAGCTGGTGGGATCGTCGGAGTTGGTGGGATCGTCGGAGTTGGCGGGATCGTTGGAGTTGGCGGGATCGTTGGAGTTGGAGGGATCGTTGGAGTTGGTGGAATCGTTGGAGTTGGTGGGATCGTTGGGATCTCTGATTCTCATGGTGTAGTATGTGGTTTTTCTGGAGTTGGAGTCGGCTTTGGCGTTGGCTCTGGTTTTGGCTCCGGTTTTGGAGTTGGCTCTGGTTTTGGCGTTGGCTCTGGTTTTGGAGTTGGTTTTGGAGTTGGTTTTTTCTCTGGTTCTGGAGTTGGCTCTGGCCTTGGCGTTTCCACTTTTGGAGTTGGTGTTGGTGTTGGCTCTGGTGTTGGTGTTGGCTCTGGTTTTGGCGTTTCTTTCTCCTTCTTTCCTCCTCAGAAGCTGATACCAAGATTCACATCCTGTTGCTCAATACCTGCCTGTGCCATCGTCTCTCAGACATTCCATGTCATCGTTCCTTGAGCCTCATCGTCCATATGCACTTTATCTATCACTTTGAGTCCTTTGATCTTGAGACCAATAGATTCATTGGCACACTCTCCCATCAGATAAAATACGAACTCGGTTTCTAATTTATATATTTTTCATCAAATGTCAAGAGCTTTTCAGGCGATTAATCCCTCTATCATTTCATTTGAAATGTTATTTTTTTGTCCTTCAGGTGCCACAGTAGCAACGAAGTTTCTGACTGTCTCATAGACTTTTGCTTTCTGAAGTGGGTATTTATCAAGGTTTCCTGACTGTGCCTCCTTGTCTCCCTCAGCTTTTTTTCAGAAGAATCGCTCTTTGGCAGCCTGCTCCTGATCTCCAGTCAGCTTTTGCATTTTTCCTCCAAGCACCTTTGTCTCTCCTAGAGCGGTAAGAGAAAGTCCTCTTCCTTCCTGGGTTCCCTTTCTATAGAAGGCGGTGAATCCTACCAAGCCTGGATCTACTACTTGATCGATATTTTCGAGCGTATTGGCAAATGCCTTGCTATCTATATTGATTCCAAGCTGAGAAAGTGTTTTTCCATTTGGACCCATTAGATCTTGATATGCATTATTTCTCTTATCTACGATTGCCTTGAGTGAAGAAAGGTCAGTAATCTTTCTCTCTGTGGCAAAAATTGCTTTTAGTCTATCAATGATAAGGTATTTATTTACTTCTGCCTTAGCGATACTTTCAGCTACTCCTGCCATATTATTACTTGCAGCATTTACTACGAGATCCTCTCGCTTTACCGCTGCTTTTTCCAAGAAAGCTCTCAAATCTTTAAACATTTCTTCTTGTGGTAAGATTCACAAAAATTCTTGAGCTGCTTTTTGATAATCATTCTTGGTCGCTCCTGTATTTGCAAGGAAGCTATTGAGTGCTGTAAGATGCGTATCATCCAGCTTTGAAAGTGCTTGCACTAATGGAGAATTAGGATCTCTGAGGTCGGCAAGCAGTTTATTTTCATTTTTGATTTCAAAGGTTCAGGTATTTGCCTCCTTATCATTGAATCTGAATTCGATTCAGGTTTTCCCTGCTTCAGAAGGTCAGATTTGAACATCAATACCTCCTATCTCATTTTTAGTAATGATAAAAGTTTGTCCCTGCTCAAACTGGTATTCTACCCCTGGTTCAAATGGTCATGGACCAACCTTTTCAGTTCCGTTGAAGAAAGCTAGATTTCACTGCTCATCGATCTTCATAGTAAGGTCTTTAAGCTCTGGATTATTAGCTTTAATCTGGTCTAAAGCCTTCTTGGCATTCGACTCCAATACTGCTCCGATATATTCTTTATTACCTTCAAAAGCTTTGACGCTAGCTGGAGTCAGTGTTCTCATCTTTCCATCTCTATTATCTTTTCCTCCGATATCGAGAATATTGGTTGCTCCTGTCGTTCTCGCAGCAGTAAGGAAACGGTAATCCGTATTTGCTGGAAACTTCAAGATACCATTCTCATTTTTTACGTATCCTTTAAGGTCATCATGAGCTCTAACTGTCAAACCAGCCTTTCTCAATACTGTAACTGGGATTTCAACGAAGTCTCCATTTTTTTTGATTTCTTCCTGCTGAGTCAAGATATTTGCCTTTCTCAGAATATTTGTCACTTCAGTAAAATCTCAATCTTCAACACCAGGAAGAGTTTCGTTTCCGATACCATATTCTGCACGCTGTCTGAGGTTTTTGAGACTATTTTTGGTGTCTTCATATCCTTCATTGTGATACTTACTAAGCTTAACACTTAGTGTTCCCACTGGCCAGAATCCTGCCAAGAATTGAACTCCTAGTGCTGCTCCCGTAGGTTTCCATCCTTTTGGAAGTCCTTGGATAGCATTATTTCTCCAGCTATCAGCATATCGCTCTGCTATAAGCTTTGCTGCATCATCTTTCTCAATTCCGTCAACAAGAGCAAAAACTGAAGTCAGATTTTCTGCTGCTTTCTGGAGAGATGCCGTATCAGATTCTGGATATTTTTGCTGTAAGTATTTTTTTACTGCATCAAAAGTAGGTTTTCCTTCTTTATCAAGCGATAAAAGGTCTTGCATCATTTGCACTCCTTCTTCTTTTATCTTTTTGTAGTGCTTTTCGATACCTTTAAGCTGAGATTTCTCAAATCCTACATTGAGTCCCCATGATGGGATTGGTCCAATGAGCGTCACGTTTCATCAGAAAGTAACTGACTGAAGTGCCTTTGGATCGATATTACCAAGGACAGATTCAGCATTTATTTCTTGTTTGATATTAGCATTCAATCCAAGTAATGGGATCGCTGCTATATTTGTACCAGCAGAAGCTGCAACATTTAGTTTTCCTTCATTTTCTCAAAGGTTCCACTTGGCTGCTTCTTTATTCCAAGCGAGTCCTACCCCTGCAAAAGGTTTTCCATCTTTGGTAACTCCTGTTCCCAGATTGAAAGAAAGTCCTTTGAGAATCTGATCTAATGGCAAATTCATTCCAGCACTTACTCCGCTGAGGTTTGCAGAAGTTGAAACAATATCCTTTTTCTTCATTTCTGCAAGTAGGTAAGAAGCAGTGCTAGACAAGACAAGTTCTTTCCCACTCTCTGAGAGCCCCTGAAGTGCAGGAGCTGCATCAATTGTTTCCTTGATCAACTTAATATCCTCTGGAGAGAATTTCTGCATCTCGAGCGTTTTTTTCACGGCGTCCTTTCCATATTCGAAAATTTTACCTGTTTCTGCTGGAGAGTCCATAAGTGCTGTCTGCACTGCACGAGCGTTCTCTACGTCAGCACTCATCCATTGTGCAAATTCCGCAGGAGTCTTTACTTTAGAAAGGTCTGATTTTGGCATTCTAACCTTCATAAAGTCAATCACATTTTCTACCACAGCATCCATTGGCACTCCTCTATTGAGAGCTACAGAAAGTGCATTTTCTACCTGCATTCCTCTTTTCACTCCACGTCCATCCAGATAATTTCCAATTCCATCAAAATTTGTATCTGTCATCAGAATTTTAATCTGTTCTTCTGGAGATTTGGATTTGATTGCATCAGTAAGAACTCTGAATTTTCCAAGATCGGACTTTTGGATTCCTTTTTGATCAAGGTCGTTGAGATGCTGTTCCCACTTACTATCAATATCTTTGTTGATGAGCTCCTCCGCAGCAGCTCTCGCTGATCCCTGACTTAGTCCCTGTTCTAGGTATTTTTTGGTCCAGTGTGCTCTTGCTGATTCCAACTGCTGAGCATTGTTCTCTCTATAGGCACTCTTTCGATTAGCCTCTTCATTACGAAGCCCTGCTTTTCTCTCTTTTTTGGAGATTGGTGCATTAAGTGATTCTGTATAGACAGCGCGGAGTTCTTTTCCCTCGTTGATAAGACCAACTTCGAGATTTCCAATGATTTGATTCCATCTAGAAAGCTCTGAATTAGATTTTTTTGCCTCGCGATATGCTAAAAGTCCTTTGTCAAAATTATCGGACTCTTTCTTGGTCCAAGTGCTACCATCCCTCTTAGGAAGAATCGTATTCCAGTCTGGAAGTGTTGTCTGATCATGAAGCAAAGAAACAAAATCTTTGGCAGAAAGACCACTTTGAACAAAAGCCTTTCTAAAAGCTGATTTGTAGCTTTTTTCCTGTTTATTGAGCTTTCCCTCAGCAAGGGTTTTTCCGTTGATAATTTTATCAAAATTCTTGAGTTCAGTATCAGAAACGGTATTGTTTTTCACCCTCTGGAGGTAATTCTCTGCCTTGTAGGATGCTGAGTTTTCTATTTCTCCTCTTAGGACATCGGCTTCTACTTTAGCCTCTTCGATTGCTCTTTTGATTTCAGCATTTTTTTGCTTTTCGAGCTTATTTTGCTCTTTATATTCTTTAAAGAGAAGCTGGAGAGCTGCCTTTTCTTGGTCTGTTTTGGCAGATTTGTAGAGCTGCTTGACCTGCTCTTTGGTCAACTTATTGAAAAAACTTTCCAGTCTCTTCTGAATGTCATGTTTTAGCACTTTTTTAAAATGCTTTTCGTTGATCAAGCCCTCTTCCCTCATTTTCATCAAATCCTGAAGCGCTTTGGTTGCCGAATCATCTGCAAGCTGCTCTTGGGTGTTTGAGGCTTCTACACCGACATAGATCTTCTTGTCTTGTGCTGAGTCCTGTGATCCATTTTTTACTGTCATCTTTATTTCAATCAAAGTGATAAAAGTATATCTTTATGTTTTATTACCCTTCTTATTGTTCAGAGAGAATCGAATCTAACTCTGAAGAGAAGTTGTTTGCTTCTGCGTTTTGTTCTTGTTGTTTGAGTTTTTCTACGACAGAGATCGCTCTTTGAAGTTTTCTCTTTTTTTGTTCATCAGAAGTGGATTGCACTACCTTTTTAAAGACCTGGAAAAGCTCATCAATTTGTTCTTCTCTGAGGATATTATACCTCATTAATACGAGAAAACCTCTCGCTGGCTCCCAGACTTCTTTTACTTTTTCTAAGAAGGTAGTGATGTACTCTTGTTTTGTCATTGTTTGCATGGCTTGTATGCTAAAATGCTTTAACTCAAGTATAATCCATTTTTTTTATTTTTCTGCTTTTTCCGCTTTTTTGGAAAATTATATACTTGCCTTTTTCCCTTTTTGTGATATAAAATAAAAAATCCTGAATGTTTTTTCAGGATTCTCTATGGATAAAGCTAGTTTTTATCTAGCCAAAATTGCTTGCTTCGTCTGAGCGAAGATTTCTTCTACGGTTTGCTGAGCGTCGATCGTAATCAGCTTTCCAATACTTTTGAAATAGGCGATCACAGGATAGGTTTCCTCCTCAAAGAGGTCCAATCTCTGATTAATCGTATCCATATTCAGATCATCAATACGATTTTGAATTTTTGCTCTGGAAAGGATCCTCTCTACTGCCTTTTCTCTCGGGATATCAAAGTAAACCCCGAGGAACTCTCTTTTGTACTTACATTCCTTACTAAGAAAATAATACATCTGAGGGATCGTCCTTGGGAAGCCATCCGCCAAGATAAGATCATTGTCTGTTAAGAGATGATAATACATATTAAAGAGATCAAAAACCAGATTATCATCCAGCATTTTACCCTCTCTGATACGTCCTTTAATATACTCTGAAATAATGTTGTTATTGGAAGTGATTGCCCTAAATACGTTTCATGATTCGAGATATTTGAGGGCTGGAAACTCTTTGAGGAGTAATTGTGCTTGCGTCCCCTTTCCTGATCCTTGGATCCCAAAAAGGATGATGTCTTTTTGCATACTGTGGATGATATAGAATTTAAAAAGAAGCTTAAATAAGTATATATTAACTGAGCTAGGATGCAAGTTTTTTTTGAATAAAGTCTAAAATCTGCTCTTTCCCTTGCCTTTTGGTCGTGGAAGAAAGGAGCATCTCTGGGAGTTTACCGATTTGCTTTTGGAGAGCCTGTTTGATGAGTTTAAGGTTTTGATGCAGAGTTTTTTGATTTGCTTTATCGGTCTTGGTCAAAATGAGTGCAAAATTCAGATTTTCTGCTGTAAGTGCCGATACAAACTCTAGATCAATCTTTTGAGGCGGAATCGAACCGTCAATGAGCAGGAGAATAAAAGGCTTCCTCTCTAGGAAATAGTGATGAATTTCATCTATCCATTTCCTCCTCTGCTCCAGACTCGCCTTTGCATAGCCATATCCTGGCAAATCTACGAAATATCGGTTTTCATTCACGAGAAAATAATTGATGAGCTGGGTTTTTCAAGGTTTATCAGAAGCTTTCGCAATAGTTTTGCCCATTAAGAAGTTGATGAGCGAAGATTTCCCGACATTAGATCTTCCAATCACCGCAACCTCTGGGAAATTTGAGCTAGGACATTCAGTGATTTTGGAAGCTGATTTTACAAAGCTAAGCGATTGAATCAGCATAAAGAATGATTGAGAATAAAAATTAAACTTCTTTATAGACCTGCCCTCTTGGACCAGCATCAAAGAAAGAAATAATCAGCTTTTGATCAAGTATCTCATAAAGGAATCTCCACTTTCCGATCCTAAGCCTATAACAATGAGGCAAACCAACAAGGACTTTGATATCCAAGGTATTCTGATAGGGATCAAGCGCCAGTTGAGTCAAAGCCTGCTTAAACTGAGTAATAATTGGCTGCCCTTGATGTTTCAATAGGAATTTTTGGACCTTCTTTTCTAAATAAATCTGATACATGTCCTATTGGAAAGCATCTAAAAAAGCGAAGGCTTCTACTGCCTCCTCTCCATGTGAGCTTGCCTGATAAGCCTTTATTTCCTGAGGAGTTGCTTTTTCAGCAGGGAAACTCAGCTTAAGAAGTGCATTATATTCATCAATACTGAGAAAAACCCCAACCGGCTTATTCTGTGAAAGGATAATTTTTTTTCCTGTCGTATTGACCTGTTTCACGATACTTGCCGTCTTATCTCTGAGATCTGAAATAGAAGCAAAACTGCTAGCTGTAAGCATTTCTTTTTTTTAGCACATAAAATCTCAGTAATTTTATAGGTATTTCTACCAAGATTTCAAGAAAAAAAATCTGACCGAAAACCATGAATCAGACTTTTTTAAATTTCTCTTGTACAATTAAAACTCACACTCAACAAGAACTGGAAGATGATCGCTCCATTCTGTAGGAATAATCTGTGATGAAAGAACTTTAATATTCTTAGAAGCAAAAACATAATCCAATCTCCATCTGAGTTCTTTTTCTTCAAAACCCATAAAAGAAAAAGGCTTCGTCGTTCGAGTTGCTTGTGAATAATCAGGTCCACAATGCTGAAGCTCTTTTTCTAGCATTTGAAGAGATGTACTTTCTGGAGGCAAATTAAGATCACCTGTAAAGATGAGATTCTCTGGATACTGCTTAAAACACTCAATCAGTTTTTTAGTTTCATCTATTTTATCTTGATCTTCTGCAAATGCTGAACTATAAGACTTATGAGCTGTAGCAACGGTCAAAAGAGTATCTTCTGCCTGTATTCTCGCAACAGCACAAGTTCTTCTTTCGTAGGGATGATCGGGATTATCTTCAGGATTGATAAGAGCAAAATCAAAGCTTTCTACGATAGGGAATCTTGAAAAAATCGCATTTCCTCAAAAGTTTACCTCTCCTAGGGGAGTAAAAGGGTACTCATATTTATGGGCTTTTGAATAATAGTAATGATACCCTAATTCTTGAGCAATAAACTGAGCAACATCTCTTTTATCATTAAATCACGAACCTACCGTAATCTCCTGACAACAGATAATATCAGGATTAAGCTCTTTCATCAAAGTAAGTAGATTTTCAGCTCTTTCCCTAAACCACACATTTCGTTGTAAAACTTTTACTTTCATTCTACAATTTATTAGATAATAAAGCTGCCCTGCAAAATACAAGATTACACCTGCAACTTTCCCTTTATTGAGATAGGATAGTAGTTTCTTTATATATTTTCTTCCTAGTTTTTCAAGAAAAAAAAGCTGAACCAACGTCAGCTTTCTTATCTTTTTATCATTTTTTAGAGGTCTTTATCTTTCTTATCATCATTCCCGCCCCATTTTTCGAGAGAGAAAACACAAGCAAAAGCTACGAAGTTAACGATAAGAATCGTAGAAATCACTTTCCAATAGAAGTCATCCGCTTCCATTAAATTCCAAATTGTCAGATACGAAAGAAAAGCAACAACCAAAATACTAATAGTTCCAAGCCAGATTATGCTATTTAAGGTAGCGGGATTTACTTTGGAAAATTTTTCATATCTAACCGGCAAAACGGTAATCCCCCAGAATGCCAACAAAGTTACTCAATACGTAGTAAGTCCTTGTAGGATTACGTCTCCTCCAAAGTTTCATCGAATAGATAATAGCGATAAAAGCCCAATACACAAACCACCTCCAATTGCGACATAAAGAAAGAATTTGTTTGTTCCTCCAAATTTTGTCATAGGTATTTATTAAATAATAAAAGATGGCTGATTATAATTAAAAGTCTTTATAAGTCAAAAAAATCTGAATGCCTTTTAGATATAAGTCTCATCCTTCAGACTCCTTTTATTCTCTATTCTCGCCTTCATAAACACTACGAAAAGAGATAAAACGAAGCTTTGGTTTACTTTGCTTTTGGAGAGTTTTTTTCTTGGTAGTATTCATAGGCGTTATATCCTGCACAAATGAGGAAAAGGATTCCTAACACGAGGAAAACTCCTACAATGAGAATTTAACCTGGTATCAATAGAATCTTCCATTCTTTTTCCCATAGAGGAGGAAGGAATCTCCTAAGAAACGGAGCGAAAATACTCCTGCCGAACAACATCCATCGTGAATAACCTCCTGAAATGGTGTTCTGTAACTTTTTCCAGCAAGAGAGTAATATATTTTTCCATCTTTTTGGTAGAAGTAGAAGAGTTTGCCTTTGAGTTGCCTGAGCTCGAAGGCATCGGTTCGCCCCTCGGATGCAATCAGATTTTTTCTATTATGAATCAAATGAAACTCAAGTTTTTCCTGCTCTAGGTTGAGCGCAATATAGGAAATCCACCGTCCTCCTTCATTAACCTCAAGAGAGAGCAAGGGTTCGCCTGTTGGATGATCGGCACTGAAAGAATACAGCAAGCGATCATTCTCGAAGAGCTGGTGCGTCATCGTATTCGGATCAGTCGTCAGATAATAGACCTTTTTTTTGAAGAGCTTTGGAAGAGCCGAAGTCGTTTCGACTGCTTTCCAGGCGTCATCAATGAAACATAGATAGGCGTCTCTCTTTGAACAGAGGATAATATGCGTCTTATCGGTCAGAAAGTAAAAAAGGTTATCGCCAGTTGCGATGAGCTGATCTTTGCGATAGAGCAACCACTTATTTCATGAGGTTTCAAAAGAAAAGTCTGATCCCTCTAATGCCTCCTTAATTGTAGTGTCAGAAAGAGCGTTAAGCTCTTGTCTCTGGTAGCGGTCGATCTCTCCGGAAAAATATTCATCAAGCGGGCTATAGAACTCCTGATCCAGCTCCCTTAATGATGCCCAACTTCCTGTGATCTCGTAAAAATCGAGTGGCAAAGCTGATTCTGGATAGTGAAACGGAGTACACGCACCAAGAATGAGAAGAAAAAAGCATCAGATCCACCTCATCGTCGGTTTGGTTTGTGGGATAAAAAGGGAGCTACTCCTCAGAATTAGAATATAGCGATTTTGGGGGGAATTAAAAGATATGGTCAGATTTTTTCTGTTTTTTTATGATTTCTAGATAACCAAGATGATGCTGATGTTCGCCGTAAGGAGGATGGCTGTCTTGGAGGTATTTTTCTTCGCAATGCGTGAGCGTATAGCCCTCGGGCAACTGAAAATCCTCAAAGGTATAGAAAGAAAGCTTTTGATTGACTCCCATCGTCTGATCATCGAAAAAGAAAAAGGTCAAACAAAGTCTTCCTTCAGGATTCGGCTGTGAAAGAGCATAAGGAAGCAATTGAGAGAGAACAAAACTCTTTTTGAGAAAGATGATAACATTGAAGAGCAAGATTAGATCGTATCTTTGGTCGCAAGGACGAGGCAACTCCTTTAAATCAGCTTGAAAGAAATGTATCGCTGGATGAAGAGTCAAATACTCAGGGAATTGCCGATCAGGCTTGGATTGATTGTCGATTGCGACTACTTTCGCACCATAGCTCGCACAGAAAATACTCCACCTTCCATCGCCTGCTCCGAGATCAAGCACTGCAGAAATTTTTTGGTGATGGAAGAGTGTTGCTAGCTTTTCTTCTAATCGCATTTCTTGGGTCTTTAAAAAAGCTAAATCCTACCCTACAAAATGCAAAATCACGCCTGCAACCTTCTGCACATTTACAATCTCCAAACGGCGACCATCATCTAGCTCAATCACTCCAGCATCTCCATTCTGACCTCCCATCTCAGGATAGAACGGTGTCTTATCAAAAATCAGCACTTTCTGCCCCTCTTCGGTATCAATCTCCTTCAAAAGCTTCGCATCAGAAAAATTCAGGTTCTCATACCCCACAAACTCTGTCGCAGGAACTCCCTCCAAATACTTAGACCAATCTGCAGATTTCTGGAACATCGCCTTGCTTCCTTGGCGAGATTTCTCTTTTGCTGCTTCTAAAGCCTTTTCATAGCCTGCGAGATCCAAAGCAACTCCACGATCCGCAGCAAGTTCTTTCGTAATCTCTAATGGGAAACCGTAGGTATCATAGAGCATAAAAATCTGATCTCCTCCGAGCGTTTTCTTCCCTTCGGTAGTAAGCTTATCCAAGAGTTCAGTCAGAATTTTTTCTCCATTATGAATGGTTTTCTCAAACTGAGCGATCTCCTGAAGGAGCACCTTTTTGATCATCGGCTCATTAAACTCTCTAAGCCCTTTGAACGCCACGAAAGCCTGATCTATAAAATGCTCCAGCTCCGGCTTCATCATCTTTCTAAGCAAAAAGAGATTGTAATACCCTCTCCTGATAATCATCCTCAAAACATAGCCTGCTCCGACATTGGAAGGCGTAAGCCCATCGTTAATCAGCATAAACGCTGTTCTCAAATGATCAGCAATAATTCTGAATTTTCTCTGGTGGTCAGCATACTTGAGTTCGGTTGCCTGTTCTAGCATATGCAGGAAAGGGGCGAAAAGATCGGTTTCATAAACGCTTTCCTTGTGCTGAAGAACCTTACACATTCTTTCTAGTCCCATTCCGGTATCTACATTCTGCTGAGAAAGCTTGGTCAGGATTCCCTTTTCATCTCTATAAAACTCCATAAATACATTATTCCAGATTTCTAGCCAATTGGCTTCATCGGTTTTTACATTAGAACCAACAGGTGGAAATTCTGATTCCCCCACTCGGTAGTAAATCTCCGTATCAGGACCGCAAGGACCAACAGGACCTGGACTCCATCGGTTATTCTTAGCATCCAAAAAAGAAATCCTCTCCTCAGGAAGCCCTGCTTTTTTCCAAGCCTCAAAAGCGATCTCATCTCTCGGAGCATCTTCATTCCCCTCAAACACGGTTACGGCTAATTTACGAGGATCAAAATTCAGCTTCTCTACGAGGAACTCATAACTCCATTGAATTGCCTCCTGCTTGAAATAATCACCCAAGCTCCAGTTTCCCATCATCTCAAAGAAAGTTAGATGTGAACTATCTCCAACCTCATCAATATCCACCGTTCTCACGCATTTTTGGATATTAAAAAGCCTATGTCCAAGCGGATGCTCTTTCCCAGCAAGATAAGGAATAAGCGGCTGCATTCCCGCAACATTGAAAAGCGCAGTAGATTCCTTGTCTGCGATAAGTGAGGCTTCTGGTAAATGCGTATGTGCTTTAGAAGTCCAAAAATCTGATCGTCTTTGTCTGATTTCCTGAGAAGTAAGGGATTGCATCGTTGCTATGATGAAAAATAAAGAGGAGAAGTCTGACTTTTTCTCGTGAAAAGCGAGACCACAAACTGCAATAAAAAATAGCTATCTCCTGCTCAAAAGCAAGGACTTCTTTATTGGTGAGAGAATGTTGGGATCAGGACTATTCTTGGGAAGCATTGTTACGCACTAAGTCCTGCAATTAGCAAATACGAAGGGACTATCTCTAATATTTCTCGTAGAATTGCTTTAGAGTTGGATTTCACAGGTTTCGATATTAAAGGTTCCTATAATCAGATGTTTGCCTTCAACCGCAGCATTGTAATTCTCCTCGGTATCAATAAGTCAAGTATTACTTCTGTAAGGCTCGATCAGTTTTCAGTTCGTCAGCCTTTTTACAATCATTTTGTCCCCCACTAGGTCATGGACGACCCATGCAGAACGTTCTGTAATCTCATTATAACTTTCACTTTCTCTGCTCGTCCCAAGCCTATATACTTTTTTATTATCTACGACTACGATAGGATCCCAAACCTCTCACGTCATTGAAGCTTCAGTATGAAAAAAGAAATGCTTATCATTATCTCCTCGAACTTTTGGCACTAAGTCCCTGCAAAGTTCTATTTCTTCACACTTTGTCATCTGTTGAATCACGATTTCATCTTCTGTATTATTGTCCAAGTTAACGAGAAGGGAAGTAAAATACGGATTATGCTGTAAATCGTGCTTTTTTACAAAGGACTCTAAGGCTTTTTCTTTTGGGCGTCTCAGTTCCTCATAGTCATAGTCACCGTCAGTGCAAAGAACCTTTCCTTTTTCCGGATCATAGGGCACAACTCAGCCTCTGGTAATAAGATTCTTCCATAAAGAGATAGGAGAACTCATTCCTCCCCCTACTCAAAAAGGAGCATAAAAGAAATGTCCTTTCTGATACTTGCAATTCCTTGCAACATCCTCTATATTTTTGATGTCCGCTTCAGGGAAAGGCTTGATATCTAACGGTTCACAGGGAGGAATAGGCTTTTGCAATTCCGCAAGGTTTGGTGTATCACAGGTTCCTCCGGTGAATGCTTGAGTAAGCTGTTCCCCTGAATGAGAGAGCATTTCCTCTTGAGGAACTGGCTGAGCCGTTGGTCAACAACCTGCGAATATCAAAGTTATCGCGAGAAAGCTGAGGGAAAGGGATATATACTTCATCTTTAAGCGGAACAATAAAGTAAAACTCCTAGTCAATATACAAAAACCATGATTAAAAACAAGCGTTTGAGCGACACTCTTCGATTAATTTGATTTCTTTTAGGTTGAGATGAAATAGTTTTTTTGATGAAAGCTCGGTAAAACTTTTCCTATCCAGATAACAAAAAAAAGCCACAACACGTGGCTCTTTTCTATTCTTTATGAAGATTTCTAGTATTCTACTTGCACACCAGGACTCATTGTCGTAGCTACGACAACCTTCTTTACGAGTTTTCCTTTTACTCCTGTAGGTTTATTTTCTTCAATAGCTTTCATCAAAACATCAATATTTTCCACGAGCTTTGCAAGAGGAAGTTGGAGATTTTAGAGGAAAAGAAAAAATCCCTCACGAAGAGGGACTGATTTTATTTTCAAAGAATAGTAGTCAAAGTGATTCAAAGGTTTTTACATTGCTTGAGTGGAAGCTTCTTTTGCTTGAGTTTACACTCTTCATTCTTTGGACAAAGGTTTTCTCGATGGAAACAAGCATTTAATCTCCATGATCCATCTTTTTGAAGCTCATATTCAGGAGCAAGATAATGATTCTCTTTCTCCATATCTTTGGGAAGCGTATAGTAAAGTGTATGATTACGAAAGGTGAGAACTAACGCATTGAGCATCTCATAGTTATGAAACAGTTCCCGATCTGGGACATCAATCGTTATAAAAGGCTGCCAGTGCGTATCTCCCTTTTTGAGAATTCCTCCATCTTCTGAAAACAAATAATCACCGACTGCTAGTAGTCCTCCAGGCACTCTACCAAAATTATCTAAAGACACGAAATGTCCGAGGGGTTCTGCATCCGTTTTAAAAGTTCCTCTCTTCTCCTTTTTTAAAGCTTGCCAAACCTCACTAAAACTAATTTTAGACTTTATAAATTTTCTTCCTAGCACCGTTCTCAACATATTTTCTCCGATTCGCTTCATCTCTTGAGTAGAGCCTGACAGTTCCCAATTATGAAAAAAGGATTGAAGTTGTTGATGAAATTGATCTTTATGAGAAGCTTTATTATACAGTCAAATAGTAATACCTATAATATTAGAAAAGGTATCATGGAGCTTCTCCCTTTCTTCAGTAGTTCCAGAGTAATAGCTGAGATCTACCTGCTGATACGGAGAAGGAACAAACTTCATCAGATTGCAATAAGGAAGCCAAAAAGGATCAAAATCACTTCCTTCCTTCAAATGCTCTCCTTTTAAGCAAGCAAGCTGAAGAGATAAGTGGAGATCGTTTGTCCCTCCATAGAACTCATCTATGGGAATAGGGAAAAAATATTGATAATCCTCGTCTTTTGCGAAGAGTGAAAAATTTAGAAATCAAATCACCAGCATAATACTTAGAAATATCTTCCTCATCGTATAAAGTATATAATAAATCTCTGAAACAGAGTAAGTGTATTCTTTTAAGAGCCAAGTGTAAGCCTATTTTGAGAACTAAGGGGTAAATGCTTAAGAAACATGCTCCCCTGAATGAGAGAGCGGTTCGGCTCCACGAACGCGTTGAACCGTTGGTCAACATCCCACATATATCAAAGCCATTGCAAGGAAAGTAAGAGAAAGGGATATATACTTCATCTTTAAGGAGAACAATTAAAAAAAATTAAAATTCTCTATACCTATAAGGAAAACACAATCTAAAACAAGTGCTTGATCCACACTCTTCGATTAATTTGATTTCTTTTAGGTTGAGATGAAATAGTTTTTTTAATGAAAGCTTGGTAAAACTTTTCCTACCCAGATAACAAAAAAAAGCCACAACATGTGGCTCTTTTCTATTCTTCATGAAGATTTCTAGTATTCTACTTGCACACCAGGACTCATTGTCGTAGCTACGACAACTTTCTTTACGAGTTTTCCTTTTACTCCTGTAGGTTTATTTTCTTCAATAGCTTTCATCAAAGCATCAATATTTTCTACGAGCTTTGCATTATCAAAGGAAAGCTTTCCTACCAATGCATGGATGTTTCCCGTCTTATCAAGTCTGAATTCAATCTTTCCCTTTTTCGTCTCTTCCACAGCTTGAGCGATATTTACTGTTACTGTTCCAGCTTTTGGAGAAGGCATAAGTCCTTTAGGTCCGAGCTGTTTCGCTACAGGAGCGAGTTCTCTGATCAAATCAGGCGTAGTAATGAGCACATCAAAATCTGTCTTCCCACTTTTGATGTCGTGGAGCAAAGATTCGTATCCTGCGATGTCCGCCCCCGCTTTCTTTGCTTCTTCTGTCTTGTCATCTGTTGTAAATACTGCGACTTTCTTGGTTTTTCCTGTTCCATGAGGAAGAATCGTTGTTGCTCTAAGCATTTGATCATTGTACTTTGGGTTTGCATTAGTCTTGACTGCGAGTTCAATTCCAGCGTCAAACTTTGCGTAGTTTACCTTTTTGAGGAGTTCTACAGCCTCAGCCAAAGGATAGGCTTTTGTTTTTTCAACGAGTTTTTTCACTTCGTTGTACTTTTTTCCGTGTTTCATCAGAATAAAGAGAATAAGAA", "length": 863952, "complete": false, "type": "contig", "topology": "linear", "simple_id": "contig_1", "orig_id": "GCA_003260355.1|MPSQ01000001.1", "orig_description": "HMT-345 Absconditicoccaceae G1 bacterium HMT-345 HOT-345" }, { "id": "contig_2", "description": "[organism=Absconditicoccaceae [g1] bacterium hmt-345] [strain=HOT-345] [gcode=11] [topology=linear]", "nt": "TTGATCGCAGAGAGGGAATCAGCAACGATAGAGATTCCAGCGATACCATAGGCAATATCTACTCCATAATTGGTATCCACAAACGCCATCTGTGCTTTTTCGTAGTAGTATTTATCATGCATATAGTGGATTACATACATCGTCTTTGCATACACTCTAGCAACATTTGCCATTGCTTTTTTAAAGTTTTCTAGCACCTTATCATAGTTCAAGGTTCCATCTTCATTTTCTACAGCTTCGAAAAGTGAAGCGTCCATTACCTGCACTCCTTCATCTTCTTCTTTTCCTTCATATCAGTCTTCATGTTTATAGGGAGTGCATTTAAGGTTGGGTAACCAATCACTTAGGACCTTATGACCAACATTAGTAAAGGTCTGTAAGAAGAAATATTTCTGATTGTATCATAATGTATCCTTTATAAAACTGTCAAATCATCGCATACTATCTCCTCATTCATTATAGATGTCCCAATACCTGTTAGTGCTCAAGATTCTTGCTCCTCCTATTTCAACGGCTCAGAGTTTCCACAAAGCTCAGGCAAGAGGACTCTCTTCTCCTGTCCATATTTTTCCATAGAGAAGAAAAGTAGTATTATCAGGCATATTATCTCTATTTATATCCACAAAGAGCTTACATCCCTTAGTCTCCTCCCCAAGTTTTACTTGAAATCCATAGACTTCATTTCTATAGATGAGAGAATCTCCCGTCTGTGTTACAATTGCCCCGCTATTTGCTTCCGTAATCACAACAATCGGAGTATCATCTCCTGTCTGTGCTTGCACTTCGGCAAGAGTGAGTTTTGCCTCCTTTCAACTCAAGATTTGTGCTGGTGAGAGTTTGGCTTCCTCTCCTGTTTGAGTACTTTTTCCACTCAAACTTTCCTGTTGGATACTATCTCCATCTCCAGTCTGCGTTTTCCTCCCATTCTCAGGAATAATTTGACATCAACTCACGAGCATTACTCCAAGTATCAAGAGGAGTATTCAATACTTTCTCATATTTTCATCTTTATGACTCTAAAACAAACCTTGTGATAGCTATTTTTCCGAGAAAGTCAAGGATTTATTCCTTCCTAGAGGCTTTTATAGATGGATATAGGAAACGTTCCTTTCTCCATACAAGGTTTCTGGAGGGAAGTCAACTCCTCGGATTCGACTTTATAGGATTGTAAGAGGGCAAAAATGAGGAAGGTTTCCCTCTATTCAGTCGCAAATACTAGGAAATGGAATGGCCTTTCTCTTTTTAGAGCGTTTTGAAGGAATCCCAGGAGAAGCTTCAGACCTTTCTTTTATGAGGATAAGAAACAATGCTCTCTGCTCGCTATAAAGGATTTTTTTAATTTCTGAGGATTAAAAGAGAAAAGTAGGATCTAGATTCAGATTTCCTGCTTTTTTTAATTACTTTTTGATCAATACCACAGCAATAAAATACTCCGTCTTCTCCATATACTTGCAGAGGGAACTTCCATCAAGTCCCACTCCAGCTATAGAGTAGATTCCTTCTATTGTTTTCAATTCCATATACCAAACGGGAGGATCGAAAAATAGATCATCATAGGTTCTTCTTAGCAAAGGATCTGGATTCCGCAAAAAGCAACGTTTCCATCGTGGGAAAGATCGATATTCAGCAATCTCCTCCTCGTATCTCTTTAATGCCCAGATTATAACCTCATCGGAAAGTCCATCGTAGTCCAATGGGCGAGAGAAGTGAATTTTAACAGAGATAGGTTTTCATAATTTTATAGTATTTTTTGTTTATGATCGAGTGGATCATTCAGATTCACGAAATGATGTCAACTAAAAAGGCTCAAAAAATTCAAGTGCCACTTTGATTATGAGAATCATTCCCTAATTTGTGAAGGTTTCCTGCTTCTAAATTGGTAGAATATGGAGAGCATTTTGTTCTTTTTTTATTGCAAATAAAAATTATTTAGCTATACTTTTTTGTGGGGCAACTCATACAGGGAAGATAGGATTCCTTGCGTTTGAGAGTCAGCCTTTTTATTTTGTTTATCACTACAAACACATGGCAATTGATGTAAGAAAGTTTGTCTATGACAACATCACTCCTTATGAAGGAGATGCATCTTTCCTTGCGGGACCTACAGAAAGAACCACTCAGCTCTGGGAAGAAGCCAAAAAATGTCTTAAAGAAGAAAGAGATGGGAATGGACTTAGATCTGCTGATCCTGAAACCATTTCTACCATTACTTCTCATAAACCTGGATATATCAAGCAAGAACTTGAAACCATCGTTGGTCTTCAGACAGATGAACCTTTGAAAAGGGCTATCAAGCCTTTTGGAGGAGTAAGAGTCGTAGAAAATGCCCTCAAAGAAAGAGGAATTGAACTTTCTGCGAGAGTAAAGGATATTTTCCATTATGCGAAAAATCATAATGATGCAGTATTTTCTGCTTATGATGCAGAATCTAGGCTTTATAGATCCAAGCATATCGTGACTGGTTTGCCAGACAACTATGCAAGAGGAAGAATTATCGGTGACTATAGAAGGCTTGCTTTGTATGGAACTGATGCTTTGATTAAAGAAAAGAAAGCTGACTTTGAAACGATTTCAGGAGAGATGACTGATGCAAAGGTAAGACTCAGAGAAGAAGTTTCAATGCAGATTCAGGCACTCAAAGAAATCGCTGAAATGGCTCAGTCTTATGGATTTGATGTAACAAGACCTGCAGAAACTGCTCAGGAAGCGATCCAATGGGTATACTTTGCTTATCTTGCTGCAGTAAAAGAGCAGGATGGAGCTGCGATGTCACTTGGTAATGTTTCTTCTTTCCTTGATATTTATATTGAAAGAGATCTTCAAGCAGGGAAAATTACCGAAGCTGAAGCTCAGGAGATGATTGACCATTTTGTGATGAAACTTAGACTTGTAAGACATCTTAGACCAGGAGCTTATGATGAAATCTTCGGAGGAGATCCTACTTGGGTAACTGAATCTATCGGAGGAATGTTCCATGATGGGAAAACAAAAGTAACAAAGACTTCATTCAGATTTTTACAGACTTTGTATAATCTTGGACCTTCTCCAGAGCCAAATCTAACAGTGCTTTGGTCAGATCACTTGCCAGAAGGATTCAAAAAATTCTGCGCTCAGGTTTCTATTGATACTTCATCTATTCAGTATGAAAACGATGACCTCATGAGGCCTATAAGAGGTGGAGATGACTATGGAATTGCCTGCTGTGTATCTTACCAAGAAATCGGAAAGAGAATCCAGCATTTCGGTGCGAGATGTAATCTTGGAAAGACTTTGCTCCTTGCGATCAATGGTGGAAAAGAAGAAGATGAAGGAGTGCAGGTAATGGACGCTTCACTTTTCGAAGCTGTAGAAAATGAAGATGGAACCTTGAACTATGATAAGGTGCTAGAAAACTTTAAAAAAGCAATGGCAAATGTTGCTAGAGTGTATGCAAAGACGATGTATGTAATCCACTATATGCATGATAAATACTACTACGAAAAAGCACAGATGGCGTTTGTGGATACCAATTATGGAGTAGATATTGCCTATGGTATCGCTGGAATCTCTATCGTTGCTGATTCCCTCTCTGCGATCAAATATGCGAAAACTAAAGCGATCAGAGACGAGCAAGGAGTAGCAAAAGATTTCCAAACTGAAGGAGAATTCCCAATGTATGGAAACGATGATGATAGAGTAGACCTTATCGCAAAAGAGATTCAGGCTTTCTTTATGGATGAATTGCTCAAGCATAGAGCCTACAAAGATGCAACTCCAACCCTTTCTCTCCTTACGATTACTTCTAATGTGATGTATGGAAAGAATACTGGAGCGACTCCAGATGGTAGAAAAGCGGGAGAGCCGTTTGCTCCAGGTGCGAACCCAATGCACGGAAGAGATCATTCAGGAGCAATTGCTTCGCTCAACTCTGTTGCAAAGCTTGACTATAAAAAGTCTCAAGATGGAATCTCAAATACCTTCTCTATCGTTCCAATGTCTCTTGGAGCGACTGCTGAGGAACAGAATGAAAATCTGGTTGGGATGATGAACGGATACTTCAAGAAAGGAGCTCATCACCTCAATGTGAATGTTCTCAAGAGAGAAACCCTTATGGATGCCTACGAACATCCAGAAAACTATCCTCAACTCACAATCAGAGTATCAGGATATGCGGTGAACTTTGTAAGGCTTTCAAAAGCGCATCAGCTTGAAGTGATTGAAAGAACCTTTCACCAGAGTATGTAGTCATTGCGAGGCAAAAGCCGTGGCAATCTCTAGTATGAAATAAAAAAAAGACTGATTTCCTTCGTGGGAGTCAGTTTTTTGTTTCTAGAAGAAGAGAAAATGCCAGCCTCTCACTCAGAAAATAGCTGATGAAAAGCTGACATTTATAAAAAGTTAATCTTGTTCCTTATTTTCTTTTTTTTCTTTCCTTTTTTGGAGGAAGTTTTCTATACTTTCTCTGATTTAATCTTTGAAAATATAAAGGAGAGAAACAATGCCGTACAGAGTCCCTAGATAGTAGTAAGAAAAATATATCAATAGGCCTAGAACAATACACCATCCTATCCAGTCAAAAGTTGTGCGATAGTCCGAATACAAACGATTTGTAAGTATGTATAGCAGAAGTAATAATGATGACTGGGCACACAAGCTTAGAAAACTCCATACGAGATCGTATTTTTCTTGAGAGAACTTGATCTCTTTTTTCAGAAGCTTATTTGTCTCATCATAATAAATATGACGAGTATAATAAACTTTTTTGAATAATAGAAGCCCAAACCGGCGAGTACAGACAGCTATTCTTGAATCTTCAACCTGGATAGTTTTCCCTGTCAAGATAGCAAGAAGCTTATCGTTATCACTTCCTGGGATTGTATCCCAGTTTTTTGCAAAATTTCGAGCATTTTTTATCTCCTGGAATAGAGAATCTCTACTCGTATTTTCTGCAAATCCATTTCCTACTCCTACTATCAAGAGTAGAAAGAAAAATAAAATCTTTGTTTTCATAATTTTTTTGCACTCCTATTAAAGCTTTTATAAAGCTCAGACTTTGAATGCTTCGGTCTTTATTATTATTTAATTCTTTATTTTCATCTTTACTATACCTATTTTTTATAAAAAGTCAATTTATTTCTCCTTAAAAAGTCAATTTATTTCTCCTTAAAAATTCAAATTTAACTATACATAAAAATTTTGTATCAAAAATTATCACCAAAGCACGATAGTTTTCTCCTTGTTACCTTTGTTTTTTGTAGGATTAATTACATTTTTTGTTTGGTTCTTTCAAAAGAAAAGCATCTTTTTCAATCATATACAATAGCTTTGGTTATCCATCATGCTTTTCCTAATTCCATTAATCACCATTTATAATCTTCTAGAGTTCCTCGTTTTACTTCTTTCCGTAATAAATTCTTTTTTCCTTCATAACTTCTAAATTTCGCTATTCAAAATAATCTTCAAAAATAAGGGATATCTAATATCAATAGTGTTTCTCATGATTCTATCACATATTTCTCAACTATAACCTTATCAGGTTTGTTTCTTATTATTCTGACATCCTTTCAATATTTTGCTCATAACTGTTTATAAGTTTGTTTATGAATGCTATATTCATCCTACAATTTATAATTAATGTTACCCTCTTCCAGAGAGTTTTGAAATACTCATCCACAAAAACAACATTTGTATCTTTACCTTCACCTCATTTTCCCATCCTTTTTAGTGTGGGTCCCCCCCACATTTTTTGCAATCATGGCTAAACTACTCGATATGGAAGCTCCATCTTTGAGATTCTGCGAGCTTAGGAAGAAGTTCATTTACTTGTGCGATATTGATCGCTTCATATTTCTCTAAGAGTTGATCCAAGGTAAGAATTTTTTGTTGAGCTAGCCACTGAGAAGCCAGGAAATCTGCCATCTCGTCAGAGCTTTCAATCCCCATTTGAATGCTTCCTTTGAGGTAATTTTTTGCGTTTTTAAATTCCTCCTCATCAATCCCCTGCTTTACAAATCTATCAAGCAAAAGATTGATCTCTTGCTGTCAAAACGAGAACTTTTCTTTACTAATTCCAGATCTGATCATAAAAATTCAATATTCTTTTTTAGAAAGATGGCTTGCGGAAATATAGTAGCACAATCCTAATTCTTCCCTAATTTCTTGAAATAATCTTGAAGACATATTTCCTCCCAAAATTGTCGTTAAGATTTTGGCGGCATATTTTTCCTCTTGATCAATAGAAAATCCCGGCATGCTGAGGATGAGATGATTTTGCTCTGTACCCTTTTTGAAAAAATCAGATTTCTTTTCTGGTAATTCTCGCTGAAAATCTGGCTTCCTTCTTGTTTTTTTTGGAGGGAGCTGGGAAAAAAGTTCTGCGATGAGGCCCTCCACAGCAAGCTGATTTTCAATCTTCCCTGCAATCGTAAACAGCAAATTGTCCTTCGTATAGAGTGAATTTTTATAGCTGAGTAAATCCTTCTGAGAGACAGCTCTGATTGTATCCTCATATCCGATTATTGGTCTTCCATAACTATTTTCTCACACGAAAAATAATGCTCGCTTTTCGCGCACTGTAGCCATTGGATTATCTTGATACATTTTGAGTTCTTGAATAATTACCTCTTTTTCTTTGTGTAGTTCTTCGGGATTTATTTGTGCATTTAACATCATATCAGCCAAGAGCCTTAGTGCAACTTCAGCAAATTGAGGAGCACACTTTATATAGTAAGAAGTCTGACTTCCTCCAGTACCTGCATTAAATTCAGCGCCAATTTTATCCATTTCACCAGCAACAGCCTTTGCCGTCGTTCGGATTTTTCATCCCTTAAAAAACATATGCTCCAAAATATGAGAAATACCTGCTTCTTCTCTTGTTTCATATTCTGATCCTGCTTTTACTGAGATTTGTATGGTAAGAGAATTGAGCTCTTGCATTGGAGCCAAAATACAGTCAATCCCTCAGATTTTTTTCATTTCGTAGTGCATAGCATTTTTTTATAAGATAAAGATTAGCGTCTCTTGGATAGTAATGAAAAAAAGCTCCAACGCTAATTGGGGCTTTATACAGAACTGAAGAGTTCGCTCAGTTCAGACAGAGTTTTATTAGGCGCCCATGGGATTCGAACCCATGAACCGAGGTTTTGCAGACCTGTACAATAGACCACTCTGTCAGGGCGCCATAGTCACATATCCTTTGAAGCCGTAAAGCCTTCTTTCCGTCTCTTGTTGAGAGAGACACTTTGAGTTGTAAGGATATGATCTATTATTTCAAGCTTTTTTTACCTTATTTTATGGAGAACGGGCTACAGACACTCTCTAACTTTTTATTTCTCACGAAATACCGATAAAGAGATCCTATGTTTTCTAGCCCCAGGAGGTACTTAAGACCGCTTATTTTCTCATCATTTTCCTCATTTTACCTCTCGTCCATCCTGATGCCGGTGCTGAAACCATTTCTGAACCCCCTGCTGGCGTCCTTATATATCTGTATCCATCAGGATCTGTTCTCAACCTCGTCTCCCAACAGACTTTTCTCCCTCTAAGAAATCGGTTGTAGGTTGCACCTTTAGGATTGGTATAAATCCCAACAATTCCGAGCTCTTTTGCTTCTTCAAGCTCTAGTTTTCTTTTTGGATAGTCAAATCTCCTACTCCCACGCACTCTACTAGCTTGCTTTGCCTGCTGGGCTTTCTTCTTTGCAATTCCCTGTTTATTTGTCCTACTCGCCATTTTCTCACTATGTATGATAAAGTTGTTCTACGATTAGCGTTTATATTCAGATTTTTTCTAATTTTTAGTCTTGTGCTCTAATTGATCACTACGGGCTTATTCTTGTGCAAAAGCATATCCAATTTTGCTTTGAGAAGGACACGATACTGTATCGTATACTGCAGCACACTGAAAAGTGAGGTCGTAGCGAGATAAAGTCCAACACCTGCTTGGAAAGACCAAACAAAAGAAGCCATCATCACCGTCATCATGATGTTCATTGGAATCATCATTTTTGACATATCTGGCATTGGGACACCACCAGGCATTGCAGCCGGTTTGTTGGTTTTTTGGACCAAACTTGTAAGCCAGAACTGTAGTGCATTTAGACAAGCAACGAGAATAGTCAGAATTAAACTATTTTTTTCAAAAAGGTCAATCCCCAAAAACATTGAATGAATATTTTGTGTATCTAGATACTGATTACCAAAGCCGAAGAAGAAGCTATAAAGCCATTCATTTGGCACTTCTCCCGTAGCAAATTTTCTAATTACAGAATAAAGCCCAATGAATACTGGAAGCTGGATAAGCATAGATAAGCATCCTTTCATCGGACCAGCACCTTCTTTTTTGAGTAGTTTCATGGTCTCTTCAGAAAGCTTTTTAGGATCATCTTTGTATTTCTCTTGAAGTTCCTGCATTTTTGGTTGGATATTGGACATCCCTTTGCTCATATTTGCTCCAGCTATAGCATTTCTCAAGAGGAGAAGTCTAATAACTAGTGTCAAAAGGACAATTGCAATCCCAAGATTCCCTCAAAAAATTGCAAGGAATATGATCAAGAGGTTGAAAACTGGACGATAAAGCAATTCTGCCCATAGGATACTTAGCCAATTCATTAGTTTTTAAGCTTAATAGAGAATAAAAATCAGAATTTTATTTTTTTATTTTTCAACGAAAAACTATACCTTCATCACTTCATTTACTTTCGCTTTTACCAAGTTATCAACATTGGCAGATTGGATTTTGGTAAGTTCATCAATATTTTTTTCATTTGCCTTGTGGACGTCTTCTGAGATTTCCTTGGCAGTAAACTGCTTTTTAGTCTCAGTCATCGCATCCTGCCTGATCCCTCTGATTTGCGTTTTAGTATCTTCTCCCATTGCCTTTACCTGCTTAGCGATATGATCTCTTCTTTCTTGAGTCAAAGCAGGAATTTTCACCAAAATATGAGAACCCTGATTATCTAATCCTACTCCCAAATCTGCTTCGTAAATTGCATTCGCAATATGTTTTGAGCTTGCTTTATCTCGAGGCTCAATCTTCAATGTTGTAGTATCCATTACGGTAATATGAGCTAAGGTATTAAGCTTCATTACCCCATACTCCGTCTCCACATTTAAATTATCAACAAGCCCTGTTGTCGCTCTTCCAACCTGTAAACCTGCAAATTCTTGCTCAAGGTAGGCGATTGACTTTTTCATTTGTTGATCATAAACCGAAAGCTCCATATTTTGTCTTCTTTTACTTGATAAAAAATCTGAATTTAGTATATAAAGAAAAAATCCCTTTTTGCAAGGAAAAGTTTTTTGTTCTAGGCATTTTGAAGCGTGCCTTCGTTATATTGTTTGTGCTCTGTTTAGTATCTTTAGCTCTCAGGTGTATACTATGATCAAAGGTGGCTTGGTAAAAAATAGCTGAAAATTTGTCTTCATGATTTTAAGCACGATAGTACTGTTTTCTCAGCCAAGTTTTGCTCAGCAAAAAACTTCCACCTGTGGAGATTCCCCCGCTGTCTGCTCTGCGACACCCGAAAGAATGGTACTTTATCTCGATTTTCAGGAAGAAATATCAGAACTTATCGAAACAAATCCTTTTGAGACCGTAGTTGGAGCGATTAGTAAGGGGGAAGGAGGAATTTTTACGAATGAGCTTTTACAACTGAGTGGGATTCAGAATTTTGATGATAGTTTAGCTGGGCAAAGCCTTAAATTAGCTTATATTGCCAATACAAGATCAGCGCTCTCCCTTCTTACCAGCATCTACCTCTTTGAACTTTCTGCGATTACGACCGTTCAAGATGTGACGCTGGGGCTTTGAATCCTCCTCCATGATAGACCTATTGTAAGAGACTGGATGAAATTGCTAGATATTGAGCGAAATCTTAGCAAGACCCTTTATTATCTTGGGAAAACTGAGAACATCATGAGGACTATTTCTCAGACTGAGAAACTCAGAAATCTCTTAAAAACCTATGAAGAAAAAGGTCTTTTTGTGCAGACTGCGAATTTTCCTAGTCAAATCAAATATCTTGATCTCCTAAAAATGTTGATTAGCCTTAATATAGCGATGAAGGATTTTCTAGCCTATAATAGCGTAGATCCACTCAGGAAGTTTTCTACTCATCAAATAAAGTTTAACGAAAAATGGATTAATCAGCTTGATGACGACTATCATTGTGTTAGATGGGATCTTTGATTTAAATGTAATGTAAAGTTTAAAAATCTGACTAAAGATTTGAAAGGACTCGTCACTAGCACCAAAAATCAATGAGAGGGAAGCTGGGAACTCATCAAACAATCCCGACACGAATTGAAACAGGCGCTCTGAAACCTTCCAAAAGGAATCAAAGAAAATATCAGGTGAAAATCTAGTGATTCCTATTTGACGGAGAGAGAAAAGGAACTCCTTAGAAATGTATATGGATTAGATACTACTAGAATGACTAAGGATGAAGCTTATTCTATCATTTCACTTTGAGAGAAAACCAAATCACAACGAAAAGAACTGGCAAAGACATCAAAGAACACCTGGAAAAATGGAATCAAATCCGCATCTTCTGCCATTCAGAGTCTCAAAGATAAGTCTGTTCGCTGAAAACGCAAATCTGCTAGCAAAGAGAAGCAAACTCCTGCTGCAAAATATTTTAATGACCTCATTGCTGCCCCTATTTATTATAATAATAAAACACTTGTTTGGGTTAAAAGGCAATTTGGTCGTAGCCCAGAATCAATTGAAGAAGAACTGGAAGTAAGTAATAAAATTCCTGAACCAATCAACAGAGACTTTAATATGCTTATGATTAAAAATCTCCAAGACTTAAGCAATTCTAGACAAGAAGCTGAGCAGCTTTCTGACATTGCGAATAATTTTGCACTTACGTATCAGTTTTGAGAGCTTTCTAACTTGATAAAAATCCTGATGAACACTATCGGAAATAAAGAAAAGAATCTGAGAAAAACTATTAATACGCTTTGTACCTATCAATGTAAAAATAAATGATCGGTAGGCTGCTACGTTCAATAGGATAAAATCAGACTTTTTATTTGGTATGCTATCACTATTTATGAACTCTCCTCTTTGGAAGCAGATATTATTTGGGATAATGGGGCTACTTGTGGGTTTTGCTCCAGCTTCGGCTCAGCAAATTAATAGAGACGAGAATGACGTAGTTCCAGAGCTGGGGACCTCACTTATCTCTGCTGCCGTCACCAACCTCAATACAAAACTCTGAAGTCTGAATACCAACGTTGAGAATAAGGAGCAAATTATTCAGTCCTTTTGTATGGCTGCAAATCCCCAGTTTGCTACCGGTTTTTCTTCTAGTATTGAAAGTTATGATAGCAGCCAATCACTCTTTTTGAGCATTCTTTGTAATACCAACGGACTGGAAAACAAGTATTTTAACCAGCAGACTCAAAAGTACCTCAAAGAGCTCTCTTACAAGAAGTTAAAGTTTGATAAAGCCTGCCAAAGAAGCTATACTGAAAAGTGTAATGTTGCGGAGCTGGCTGATGACCTCCTTACTCAACTATTTTCTGAGCTATTTACTTTTAAACAGGCGGAAATTTTTTGAATCAGTCAGGATACGCTTGTTGATAGCAATCTTTTGAATAAAAAAATTGATGCCTATGCTGAAGAGAAATATAAGATTCCCCACTTCTGTACTGATAGAATCCATAATTATAGCCAGACTTGCTCTATTATAAGCAGACAAATGAAAGCTTTTGTTCCTGTAATTAAAGGATTTAAGATTCTCAACTCCGATGAACTCTATAAAAACGTAAAGGATAAAGAGTTAACTTTCCCTCCCTGTGAGATTGGACCTCACTATGATCTTGTCCTTTGTGGAACTCTAGGAGAGACGGATAAATGACTGAAGCCTTTTATCAATATGCTCTATAACGAACTTGCACGATATACGATGTTTTCAACATATTATACACAAGCACTTATGCAAAGAAGCAGAATAGAGCTTCCTACTCAAGATGAAATTAGTCTTTTGAAAGAAAATCGAGGAAAATTCTTTTTCTTGACCAATCAAACGCTAAACGACCTCACAAACCTTGCTACAACCTACCCTATTCATATTGGATTTTTGGCATATCAAGAGGATCTTCTTCGCTTGAGAGACTCCTCTCTTAGCAAGCTAGTTACCCCATTTTATACTCTCTATCATAAACTCAGAAATGTCCAGTCCGAAACTTAAATCTCATCCTTCCATTCGCTATTTTTTCTCTGGTTTGAGCCTGTTTTTATTGTTTTTTGCTATTATTTGGGATGCTAGTGCTTCTGCGACAACCTGGAAGATTGAAGGAGCAGGGCGTGCACAGCTTTTTTGACAATGGAAAGAAGCGGTAACAAGCTATCAAAACACTAAATATCAGCAGATTAAGGACATTCCTACGAATCTTTTTGGAACTAATGCTGAAAATTATGCTATTAGAAGCTTGTCTTCTGTCATTAATGGGCTTGAATCTACAAAAAAGATTCTGAATAGTCAATATGCTAATTGTGATCTCTCTGATGACAATATTTCCTCTATTTTATTTTTTTCTAATACAGGATTTTCGAAAGAGCTTAGGCGTGCTCTCCCAGAATCAAAATATCCTACAAGGAAAGAATATGAAAAAACCTGTGAAAAGCTTACTTCTTGCATAGAGGGGAAAGCTGACACGAATCTTAATAAAGCCTGTGATGATCTCATCAATACTACGTATGCTTTTTGACAGTTGACGGAAGGAAATCAACTTACCATTGAGGAAAGCAATCTTGGAAAAGATAGGTATCAAAATGGGAATTCTGAAGACTCTGCTTATGACTTATTGGAAGATATCCGCCAGATTGGAACTATTTTTTTTCAAGAGACCAAGGAAGCTCCTGAAACGGTTTTTTATAGGCTTCCTGATTTTAAGGTATGACTTCCACAAAAATCCAAGGCTTGATCATCTTCCAAAAAAGGGAATGATTCAACCCCTTCTCAGTGAGCGAACTGATCTCCTCAAAATCAGCATTCTTCTCCTGTTTCTTCTAATACTACCACTTCTACTAATTCTGCTCGCTCTCTGAGTCGTCTTAGACAAGGATCGGATGAAGAAAGTCCTTCAGATGATATTCTCCCTCTCAGTGAAGATTCTATTATTAATGCCTTCATTAAAGCGAACGATCCTATTAAGACAGTTTCTGAACAGGGGAATGTGGCTTTTGTAAATCACTGTACAATCTCTGGAAGTCCACTTATTAAGCAGGCAGAGCAAGCGGCTCATCAGCTCGAAAATAATACAAAAAGTACATCATCCTCAAAACAGCAATCTTCCTCCGTGGCGAGTTCTCCCACTCAAGTTCTTCCGAGTGCTAATGCACTTATGCTTTCTCCGCAAGAGATCAATAGTTATATCACAGATATCCTGAGAAATAGTAATAGCATCAAATCTGCTGTTTCTCCTACTCTTCGTAGAGGAGAGACAGAAAACAACACAACCTCTTTGGTTGCATCTTCGGATTCTGCTATTATTGCTGATGTCAAAAAACAGCTGGAATCTTGTAGTAATAAATGTGATGAGCTATGATGGGACGAAAGAGCTGTCTGCAAGCTGAAATGTCTCTGCTCAGACTACTCTTCTAAAAACACCCCTGAAAATACTAAATTTCACTTCTTGGAAGAAGGAGCCTTTAAACTAAGGTTCTGTACCGTTCCAAGCAAAATAACTCTTGCAAATAGTAATGTCAAAATCGTTTATAGTATTGAAGAAATCTTGGGAGAAATCCAGAAAGCTATTAATGGTCTGTATGATAGCGGAGAACTGACCACGAAGACGAGAAATAAAGAATTTCTCAATACTTCTCAATCAAAATTCAAATTCTGAGAACACATCAGTTTTGTGGCTGGTGTTGGAAGGAAGGCTCCGACTACAACAACCTATCAAAAACAGCTCCAAAAGACAGAACAAGAGCTTAATAAATCCACTAAAGATAGTATGGTTCCAGATAGTGATGAGAGGAATCGCTACCTTCTTTTGGAGGATTATACTATCACCAAGAGGGAAAGAAGCATTGAGAAACCGGAAGTTGGGCTTCCAAAACCAGCAGATCAAGCGCTTTCTACTGAGGCTCTTGAATCACAGAAAAAATATGCGTCTATCAACTTTCTTGTAAGCAATTTCCTTGATCAGAATGCAATTCTTCTGATCAACCTCAATCAAACTCTCTTGGAGATGAATGAGGCACTTAATTATCTTTTACAGAAGAAATAGGTTATTTTATTACTTGTTTGCTGAGGCAGGTTTATCAAAGTATTTTTCCACAAGTGCTGCATTAAGTTCCCTTAGGAAGGCTAGCGGATCGAGAAGGAAACTATTGAGTGTTTCCGCTTTATCATCTCTAAATACTAGTTCAAAATCCTGAACTACGACTGGGCGACGCTTTGTTCCAAAGTCAATTGCTATTGGACGAAGAACGTTTTTAACCAGATCATAGGTTCCGATAAAATGAAGCTCCCCAACTGCAAAATATACTTTTACTGTTCATCACTCAGATATTACCTTTTCTGGGATAATATCGAGGTATTTTGAGAACTTATCCGCGACCAAAAGATGCTCCTTCATTCAGAAACCAGAATTTTTTTCTTTTGGAACATAACCAATGAGAGCATTTATGATATAGAATGTCGATGAAGCATCAACAAGTGAAAAATCTATTTCTTCTAATATCTTGGTCTCAAGGACTTTATCTGAGATTTCTAGCTTGTGTAGTGCACCATTTTTTAGCCCTAAGGTATATCCTAATGGGCTCGCGGTAACTGTGCCATCAGAAAGCCTTGTTCCTGTAGCAAGAGAGATTTTTGCTTGCGTATTAGAACAAGAAAGAAGTTCTCTTCCATATTTTCCTTGGAGCTGAGAACAAAGTCTCAAGTCTAAAGGCTGTGTTTGCTCTGCGATTTGTTTGTTTTCCTGCATCAAATTTAGCACCTTATTGAGTGAGAGTCTATCATTTTTAATCAATGCGTTGAGATATTCAGTCAGCTTTGGATTCTTTGCAACTTTAATCTCAGTAACTGACAAGAGAATGTTCTCATACTTAAGCTTTGCCTCTACTGTAAGATTTGTCCTTCTATCTACCCCTTGAACGAGGAGCGTTGAAGACTCTTTGCCTTTCTTCACTGAAATTAGCGTAAATTGAGCAGGAAGATAACTACTTCTAAATAATCTCTCTAAGTCCTGTTCTTCTTTTTCTGAGTTAATCGACTGTTGATGAGGAGCCCCTGTTGATGAAGAGATTTCGTAACGGATCTGGTCTTGTAAACCAGCAAAATTAGCAATCTTTTCTCCATAATTAAGAGAGAGAAGCTGACTGGTTAATGCCGTTCTGTCTTCTTTCGTCATTTTTGAGTTTTCATCCTTGAGATAAGGAGCGATAATATTCATATTGTATCGATACGTGAAAGTCTTTGGGAATGCTCCGATGAGATCAGCATTTTTTGATTTTTCCTTAATAAGAATTTCACGAAGGAAGTTAAAGTATGACTGCATCAAGGATTTTACATCGGAAGTATTTGAAAGTTGCTTATAAATTGCTTGTTGTAAGGAAATTAGTTTGTATTCATTGAGAATCTGACTTGAATAGATTTTTGCATCGGCATACCCTAATTCTAAGGTTCTTGATAATGCAGAAAAATCCCTCAATGTAGTGAATTGGTTGTAATATTCGCTTCCACAATCATGGAAAATCTCGGAGAAACGATCATCAAGAATACCGCCATATTGTCCGTAACGCACCAATCCTTGACAGAATTTTCAGCGATAATTTTTATTTTTTCTTAGAAGCTTATAAAGGTTTGTTAACTCTTCTGAAATTTTCATCCCTCCTTTTGTATTTGGATCTTCGCTGCCTTCTTCAGAATTTTCTTCTGTTTTTAGATGCATATCATCTAGATTATACAAAAAAAACTGATCAAGGAAATTTTTGATATAGGATTTACAAACGAAAGAATACTTGGTCGCTCCTTCTACGCAATTAAGCCCAAAGGACTCGTGAATACTTCAGCTTAAGGGAAGAAGTTTGGCAGTTTCTTTAAATGAAAACTGCTGCTTTAAGGGAGCTTCTATTACTGATGCAAAAAACTTCTTTAGATAAGCATCGTTGAAAGCTTCGTCATTTTGGTGCTCAACGAAGGTATCAAGGTTTTCAAATGAAAGAGAAGTCAACTCTGGGAGAACCAACCCTTCCATTTCGAGGAGGTTATTTTCTGAGTTAAATCCTTCAGTACTTGATTCTATTTTCCCGTTTAAAATCTTTGCCGTTAACCCGGAGATAGCAAAAACGGTCTGCTGCTTATCCACATAGAAGTCTTGACTCTCTGTCGAAGAGGGAAGCGCTTCCTCTGCTATAAACTTTGGAGAAAAACAGGAAGAACATACAAAATATCCAAGTGCGACAAAAGAGAGAATAAGGCTTATAGTGGTTACTCAATACCTAAAAACTCCTTTGTATACTATGCCATACCAATGAATTATTTTTAGCTGTTCTTTATTGAAAAATATCGATTCCTTCATCAAGTCTTTTTGATAACTAAATCAGATTAAGTATAGTCAGAAATCCTGATAAGGCAAATTTTGGGGCAGAAAATGATCTTCAGAAGAAAATCGTTTCTCCTAGGGAAGAAAAGAGGAATCTAATTTTAAGTGGCTCTGTAGCCTTTAGATTAGATTTTTTTTGCCCTCTTGGATGTTGGAGAGGGATGTTTTATAGCTTCCCAATCGGCTCTAAATTCAGAAAGAAACTGTTCAATCAGCTTCGGATCAATGAGAAGGATTCAGATTTCTCTATTATTATCTAAACTATTCGCAGAGAGATTCATACTTCCAAGCAGAAGAATTTTCTTGTCAATTAGGATCATTTTCGTATGCACATAGGGCTTTTTCATCAGCCTCACTTGATGCGTTCAAAAGTAGTCGGGAAGCGACTGATTGGTCTCCGTATCGGAGAAAATCGCTTTGAAATTCAGCGTTTCTGACTTTTTTGCAATGAGATCAAAAAGGGCTGGATCATTGAGATATTGGGTCTGCATGATAATGCTCTCCTTCGCTCAGGAAATCAGCTCCGTAATCACCGCACGGCAATTGAGATTGCAAACGACAAGATTAGGATGCAGGTCGGAAGGGAGAAGTTGTTCACCTTTTCGGTCCTTTTCAAACAGCGTATGCAGACTTTTTAGCACTTCTTGGTTTTCACTTCGGAAAAGGTGTTCACGGTTCTTTGCGAAAGTGGAATGGGTAAGATTAGAACTCTGAATCCAAAATCCGCTCTGCATCAGGGTAATCTTGCTATGAAGATATTGAGTCGGCAGATGCTCATCAGATTTCACTTCAATATTTTGCAGTCCTGAAAAGTAATTTTGGAGTTCTTTATACGTATTTTTGAATTGCTGATATTTCTTGTTTTCAATCATAATCTGGATCTTCACTCCGTTTTCTGCCAGCTTTTTGAGTTTTAATTTAAGATGTTTCTCGGTAAATTCGTAGGTTTGAAGCTGGATCCTTGGGCTGTTTTGAAGGGAGCTTTCGTAGTTTTTGAGATAGTCAGGATTCGGCATCTGAACGATCGTTCCTGTGACATTTTCTACATGCTCAAACCAATCTGAAGATAATGAAAAATCTGATTTTTCCGCTCATTTTTCCTCTCCTCCAAAACGAAACTCCACTCCTGGAGAAAGTAGCAAGGCAAGAAGTGCGGCAATCAGAGCGATGAGAAGCTTTTTCATGGAATGAGAAGGAAGATAAAAGAGTGTATAATTCCTGTTATTCAAATAGATTGCCACGGCTCATTACATTCGCCTCGCAATGACCAACTACCCGATGACTAACTACCCGTCATTGCGGACGAAGTGAAGCAATCCAGTTTCTCTCACTCGTCATTGCGAACGAAGTGAAGCAATCTAGCTACTAAACCAAATCTGATTACCAGTGGATTGCCACGGCTCATTGCATTCGCCTCGCAATGACTAACTGTATAGTTCAATTATTTTATTCTAAGTATTTTGATAAATCTTTCCAGTCAGGATTTATACTTTCTATTAAACCTAGTTTTTTCTTTCTATTCCCCGCTTTAAGTTGTTTCTCCCTTTGAATTGCCTCTTCAATACTTCACCGTTCCTCATAATATACGAGTAAATCAAGATTATACTTAGCAGAAAAACCATTTACAACCTTTTCTTTGTGTTCAAAGATTCTTTTCACAAGATTACTGGTTACTCCTACATAAAGAGTCCCATTCCTTTTATTACTCAGAATATAGACATAGGCTCTTTTCATTCACATAAAATAAATAAAATTCATGTATTATGCGTTATTGCAAGCAAAGCGAAACAATCCAACTCCGCTCCGTCATTGCGAGCAAAGCGAAACAATCCAACTACTAAACCAATCTGCCTACAAGTAGATTGCCACGGCTCATTGCATTCGCCTCGCAATGACAAGTAACCCGGTCGTTTCACTCCCTCGCAAAGACAGCTAACCGTTCATTCATACTACACGATCAATCTGCTCATACAGTTCTTCCAAACTGCCATTATTATCAAGCTGAATATCTGCTAGCTTCTGGCAGGCGAGAATATTCCCTTTATTAGGGTCAGTATTATTCGCTTCCAAAGCCTCTTGCTCCTGAAAATGTTCCCAAGTAATCTGATCTTTCACAGAGTTCCTTTTTATCGCTCTTTCGTATCTGAGTCTCTGATCCGCATCTACTGAAAGAAGAAGGAAATCAGACTTCTTTTTTAGTGCTTCCACTTCTCCAACAGTGCGGATTGATTCAATGATGGCGTTCTGCCCATTTTTCTCAGCTTGCGAATAGAGCTGTTCAATACTATAAGCAGGTCAAAATTTCGCTCTTAAGCTATCAGCGAGCACTCTCATTGTGTCTCTGTTAATTTCTTGCCCTTGCGCTTGTAACTGCTCCGTGAGATAGCCACTAACCGAATAATGCACAAAACCGAGCTTCTTAACCAAGTATTCTACAACGGCCCCTTTTCAAGCTGCCTGTGGTCAAGTAATTCCGATAATTTTCATGCGATAAATATAGGAAAATAAAGCTGATATATTTACTTTTTGTATTCAAAGGAAATGTTTTTTAACTCTGATTTAAAAGCTTTTGAAGGCAGGAAAACAACCTTTTTCTTCATGGTCCTTGGATTAAAATGCTTGGTATCATCCACTCCTTCACTTGAAAGACTCAGGCGGAGCGTCCCGATCTCACCAAGCTGCACCGAATATCCGTGAGCAATCCGCTTGGGGATCTCTTCCATAAGATTCTCTAGCACATTGAGGACATCTCAAGGTGTCAGTGAAGACTTATCAGCAAGAGTCTTTGCAATCTGCCTAGTAGCAATCTTACCCGTATTGACCGAAGTCGCATACCACTTCTTACGGCTCTGATCTAGAGGATTTGCCTTTGCGACGAAACGATAGTGCATTTTAAGAGAATAAAGGAATAAAATCAGCATCTTCTTTAGTAGTTTTATTAGTTTACAAGGAGGAAAATCTGAATGCCTTTGTGAGAGTTTACCCTTCCCCAAAGGAAAGTCAAGAAAAGAAAGGGAGAATAGAAAAAATCATAGTTATGATTTTAAAAAATTGAAACTATGATTTTATAAAATTGAAGTTATGATTTTTTATATCTCTCTTGAAAGGACTTTGATTCTTACCTGTGAGATGTTTTTTTATGGAGGCAAACAAAAGGATTCTTTATTAGTCAGGAGAAGTATTGGTTATTCAATTTATAGTTGATCGTGAAGTATAGAGAATGGAACGTCCTATTGATTACCAGTTCAAGAATTAATCTTCTGAGGCAATCTCGTTAAGTTCGTATTATATTATAACCTTAAAGGAAATTTTTTAATAAAGGAGCATAAATATCTTGATCAATAGGAACAATCGTTTGAGGATAAAGATCCTCTACAGTATAATGAATATGAGGCATTCCTCTTGCAATAAGCATTGGGGTTTGTTCATTAGTTTCTCCCATAAGAAAGACTACAAAGCTACTGAGGGATTCTGGAAGATTAATCTGAGTAAGTTCCATAGTTTTTCCAAAAAGATCTTGTTCTCCTCTTTTATCAATTAGAGGATAAAATCCATAACTATACAAGGCAATTCCTGCAGTTCCATACCTTAAGGGTCTAGAAGTGGTATCAGTTATAATAATTCATAATTGATTTATTTGATATTTTTTAAGTAAGCAAGTATGTAATTTCTTTACTTCCTCTTGGTAATCTTTTGGCAATAAAATATAGTAACCATTAGCATTGCTCTCATCAATTCCAGCGGAAGGGATAAGAGTATTGTCTGTTATCGTAAGATATAATCCTCACGGAATATGTTCGTTGATAAGATATTTCTCAGACTCCTTTTCAATCAAACTCTTTTTCTCAGCTGTTCCTATTTTAACACATCTTCATTGATGAACTGCCAGAATTTTTGAAGAAATAAAAAGCAAATCTCCATTCTCTAATCAAGGAAAGGAAGTACCAAACAATGTTAAAATATCATCTTTAGGTGGAAGAAACAGACGAGTTTTTACAGGTATAAACTGCATACAAAAATATATTCAATTAAAATCCTTCATCTTTTTTAATAGTGTCCCAGATTTTTTTTGGATAGTTTGTTCTGAATTTCTCAGAAGCTGTAGAAATTCCTTTGGTGATAGGAGATTTATTGATCTCTAAAATAACTCAATCCGCAAGTTCTCCATTACTTAAAATATCTACTCAAAAATATCTTGCTCAGAAATATTTTGCCACCCTTTCTAAAAAGAGCCTATCTGTCTCAGTCGCTTGTACCTCACGCACAAAACCTCCTGTAGCAATATTTGCTGTAGGCAAGAGATTAAATTCAAAATCCTGAGGCAACACAGAATCTAAAGACAGATTCTGCTGTTGCAGATAGGTAGTAATTTTATTTATTTCAAGATCTTGAAAATCTTTATCAATAAGCTGACCTATAGAACTTATGCCATTCCCAACTACCTTTGGAGGGATTCTCTCATAAGCTGCTAAGATTTCACCATCCAAATAAATCACTCTATAGTCCTTTCATGGGATATATTGTTGTAAGAGATAGAGTCCTTTATTGCTTGCATTATAGTCTTCAAGGGCCTTTAGTAATTGTTCTTGATTGAAAATTTTTTGCACTCCAACCCCCAAACTGCCATCATTTGGTTTAAAAATCAAAGGATATCCGTTTTTTTTAGCAAAATCCAAACATGCTAAAGTATTATTTGAAGTTGTACTATAAGATGATCTATCTTTGATGATAATCATATCATTGGGTACTGGAAATTTTCAGTTCCTAAGAATCTTAGAGGTATATATCTTATCTTCAAAAATCCTTCTTAATGATGAACTATCAAATCCATAGTCAGCATTATAAATAATTCTATTTTTACCGCTTGGAAGCTGAATCGTCATAACAAAACCATACTCATCAATCGTATAAGGGAATCAACTTTTTTCAATTTCATCTAAGAGGATAAATACTCCATGCTTAATTCCATGGCTAGCATTAAGAATTTCTGTTTTATTTTGGAGATGAATAAGTGAGTGTACTTTTCTTTCAAATTCCTCCTCAGAAATTCATGCATCAAAAGATGAATCATTCTTTTTTATAACTTTGTCTTTTGTTCCTGTTGTAGCATCAATTATTTTAGACCAAAATTTTGTGTTAAATTCAGGAGAAATCTTTCTTGCCCACAATACATCAGGTTTTGCATTTAATTCTATGATTGATCCCTCATGTATTGCTCACTCACTGATAATATCTAGTCAAAAAAATCTTGCTCAGAAACTTTTTGCAATTTTTTTCAGGAACTCTTGATCTTGTTCTGTAAACGGAACTTCTTGGTCTTGTTCACTAAGATCGTTGATAAATTGGTAAGGTTGAATTTGTGTATCCTTCGCAGGTATGTCCGTAAGTAAATAGCCTTGTTTCGCTAATATTCATTTAGTTTCTTCTAAAGCTTCCTCTTTCAAATTTCTTAAATATAACAATTTTTCTAATGTTGTTTTCCCATCTCAAACTAAGGTAAATGGCTGTTTCATATATGTGAGCAGAATCTCTCCATCTAAGTAAATAACCCTATATTCATTCCCCTTAACTTTTTCCTGAATAACGGCTATCTTCTCATCAAAGAGACCATTACTATATTCTTGATAGAATTGTTCTAACTCTTTGAGATTACTAAGAAGCATAACCCCATTCCCTCTTTCTCCTTTATTCGGTTTGACAATCAAAGGAAATCAAACTTTCTCAGTAAATTGTAATAATCCTTCATAGTTATTGACTGGAGTTTCAAAGATATTCCCTTTAGGTTCCAAAATATATTCTCTAGGCAATGTAAATCCATCTTGTCTAAGAATTTCATTAGTTAAAATTTTATCCTTAAAAATCTTAACAACAGAAGTGGCATTGAGTCAAAAATTTATTCCATGCATAAGATGAGGAGTTTCCCTATTAGGAGAAAATATTCTCAAGCAATATCCATAAGGATCTAAAGAATAACTAATTTTTCTCTTTTCCAACTCTGCAATAAGATATTTTTGTGCGACAGCAATCGGAAATCTCGGATTATAGAGTTGTAAGAGTTCATCTTTATTTTTTAGAAATTTAAGCGATTGAAGATATCATAAATTCTCTTGGTATTTATCTCGATTTAGTATTTGATGGAGATTTTTTTCATAGGGCTTATTTTTCATTTGTCTGAAGGAATATTTTCTTCCTCTATTTTATTAAATTTATCCATAATTAAATATTGACAAAATAAAATTATACGATATATAATAAAATTCTTTTCTTTTTCAAGGTAAAAAAGAAAAACATAGCTTATTACTGGTATAATAGTTATCAACAGCAATATTGAAAGGAAATACAAGGACATTGATTGGGATTGTTCGCGCAGCTAATTCGTTCAAATTGACTGAAAAATTGTTGCACTCTAGAGAGTGGCATAGATACCGCTAAAGAACGAGCGGATCGTGTACTCACTGTGCTCTCCGCTCCAGAATTAGCCTTTATATACCAGTCCAAGTGTTTCTCATATTCCTCCGGAGTTGGACGCTCTACCTTAATGATAGGGATGGCTCCAACCCCACGAGTATCGTGCCCCACCGTAATTTTAAGGAGATTAAACTTACGAGGATAAACCTCCCCTGCTCCTTTTATCAAACGAGCATACTCCTCATAATGCTTAATATCATGAGGAAGATAATAGGATGCTGCTGCTGGATCAAGAATAAGCATATCGCCTTTCTCCCCTAGAGGATCTTCATAATCTGGATATAAATCTTTGTAAAAATGAACATCCATAATTAATGCCTTCGCACCTTCAGGAATAGGATATTCTACCCCATGATCTTGCTGAGAATCCAAAACTTCCACATTAGCTTCCTTTTGACAAGCGATAAAGATACCGCCTCCAATCAGAAACACTATACCTAGAGCGAAAACAAGCGTTCTCCAGCTCTTTTTTTTAACTATCCTTTCCATTTCTTTATACGTTTTAATTAATACTGATGTTAATGACCACTTCATTGCGATTGCCAGTATGATAAAACTAAAAGAGAAATCAAGGCTATTCTTCATCGCATTTTAGGAGCACTCTCAGTTTTTTGAAAGCTCTTGAAATTCTCTGTCTGATCACTTCCTGCGAAATCATCAACTGTTCCTCTATTTCAGTATTTGACTTGTGTTCTATATATTTGAGAAAAATAATTTCTCTACTCAAAGCGTCAAGTTCCTGCATCGCTTTCTTGATCTGCTCGAGTTCAAAGTCTTGCTGTAAAAACTCATCAATTACGCCCTCGTCCACAAGCGTATCTTCAAACTTTTCTCCCTCTTCATCATCACCATCTAGGAGAGAGAAGGGGAGTTCTCTATTCTTTTTGAAGGCATCTTTGACAGTATTCTTGAAAATCACCCATACATAAGCAGAAAAAGACTGATTGATATCGTATTTCGTCACTGCGTTTCGGAATTTTACATAGAAATCTGCGATAATATCTTCTGAATCCTCTTGAGAGAGAAAGAAGTTGCTCTGGAGATAACGGAAAAAAATATCAACCGTCTGGAGGTAAAAAGTATTGTAGGCTCCAGCATCCTGTTGGATTAGAAGAAGAAGAAATGCTTGTTCAAACTTAATAACAGTCATAAGGTAATTAAGAATGTAGAGTATAAGAGTAAATCTGATATTAGAAGGTGATTGCTATCCGAATACAGAACGAAAGGATTCCTATACCACATAGTCCTCCAAACCATACAAGGAAAGCTTCAGCTTTTTCCTTTTTTCTGCATATTCGGATAAGAACTTTAAAGAGGCCTATTCCTGCCCCGGCTCCTAGAGTATTGGTGACGACATCGGTCGTATCACAAGCTCCGATTGCCAAGATATACTGGCTAGCTTCAAAGATTAAACTTACGATGCAGGCATAGAGGAGCCCTTTTCGCTCTTTTCATGGGCTCGAAAGCGAAGCAGTCAGTAATCCAAAGGGAATAAAGACAAGGAAGTTTAAGATAACATCCCACAATATACCCGCCTTCATTATCTCCGAGAACGGAATCAGATTCAATCTGCGTTCCTGGAAGAATACTACTTTTGAAGGGAGATCGAGTTTAAAGACGATTCACCAAATCAACAGGAGAAGATAAATGACGAGATACCCTCTCAAGATATGCTTGGAATGAGCTGTAATCATGTCTTTCTGCAGAAGTAAAAGTGATACTTAGTAGAGTTTTTTGATCACAATGGTATGATCTTTTTCATCGGAAAAATATTCAGACTTAACCCCACTCACACAGGCAAGAAGCTGCTTTGGATGATTTCCTTCGTCCATTGCCACCTCAAAAACGTGTTCGCCCGTCTTATTAAGCTCGACTGCGTCTCCTCCTTCTACGACATCGCACAGGCAAACCCCACAAAATCCTGTTCCACAAGACTGAAGGATTTCCACTCCGTGCATTGCTGCGAGTTCACCTAAGGATTTTTGATCTTCGGCTTCAAAGGATCAGATTTCATTTCCTGTTGGATCTTGGACTTTTACAATTACGGTCATAGCTTTAAATGAAATAAAGTGCTTTGAGTGTAGCAATATGCTCATCATTATCAAGAGAAAGGAAATCTGATTCTGACTTGTATCTTTTTTTGATTTCCTTAGACTAGGTTCTGATTTTAGTTTGGGAGGAAGTAAGAGTTATGGACTTTTCTATGATTTTGGGAGAAGCAAAAGACACGATAGATCGCTTTAAGCAAGGACACCCTGATGGTGTCGTTATTATTCGATGAGCAACTGCAACAGGGAAAAGCAGGCTTTCTGTCGAGCTTTCAAAGTTTTTTGATATTGAGGTGATTTCCAGCGATAGCAGGCAAATCTTTAGGAAGATGGATATTGGGACGGATAAAATTAGTGCAGAAATCAGAGCTAAGATTCCTCATCATCAGATAGATATTGTAAATCCTGATGAACAATATACGGCTGGGCAGTGGAAACAAGCTGTAGATCAACAAATGGATACTATTCTCAAAAAAGGGAAAACACCTTTTATCGTCGGTGGGACTGGACTTTATATTGATTCAGTTTATAAGAACTTTTCTATGCCTGAATGTCCTCCAGATCCAGTATTGAGAAGAGAATTAGAAGAGAAGGAAATAGCAAATCCTGGCTTTCTTCATCGCGAACTTTCACGCATTGATCCTGAAGAAGCTCAAAAGCTTCATCCTCATTCGCTCAGGTATCTTATTAGAGCTTTGGAAATTTATTACAGTACTTGAATCACAAAAACGGAAAGTTTTAGAGAGCAAGCTCCTCTCCATCCTCTTTTGATGATTGGACTACGAAGAGATAAGGAATCCACCAATCAACTCATCAACGCAAGAATCAAAGAAATGTTTCAAAATGGGCTTGTCAATGAGGTCGAAATGCTTTTAAGCGAAGGTTATGCTCCGAGTCTTCAATCTATGCAGGGGATTGGCTACAAAGAAGTAGTTGGCTATCTTTTGAACGAATATACCTATGAAAAAATGGAAGAATTACTCAAAAGAAATACACACCACCTTGCCAAGAAGCAAAGGACTTGGTTTAGAAGATATCTTGCAGAGGGGATTCAAGCTCCAAAACCAGGCGTAAGCTACAAAGTTTTTGAACTCTAAATATATAAAAAACTGAAACCTGAACTCTCTTTTGAGTCAGGCTTTCTTTTTTTGAGTAGATTCCTTGAATCTTTCCTAGCCGACAACGAGGATTTCTGTCGAGAAGAAAAAAACTGACCTCCCCGTAATTGAGTGTCAGCTTTTTCTTTTTTACTATTCGTAAGCTTCATATTTTTGCATTACAAGATCTCCCTGGATCTTATTCATAATCTCCTGCACTACACCAACGATAATAATCACTCCTGAACCAGTAACTACCACCGGCAAAGAACCAACGGACTCTACTAGAGATTGAATAAATGGGATCCACTGAAGAATATAGGTATAAATACCGATAACTGCTAGTCCAAGTCCACCCCAAAGACAGAGATGCATTAAGATACCGTTGATATATTTAGCAGTTGCTTTTCCTGGTCTAATTCCTGGAATAAATCCTCATCTTTTCTGAATGTTATCTGAAATCCTATCAGGACTAAAGGTAATCATCGCATAAAAGAAAGTGAACAGGATGATGAAAAGCACATATACAGCAATCGCCAATACTCCTGGATTTTGAGCATAGATATTGAAATGCGACTCTATTCGATTGGCTACTGCTACAAGCTTTAAGTTTGCTGGCTGAAATTGAGTAATCAACTTTGCTATCAGCTGAGGAAAAGAAACGAAAGCCATTGCAAAGATGATAGGCACCATTCCCACAGGATTGAGAGGGATAGGAAGCGCAGATGACTGCTGAGCCTTACCCTGCTTTGCGTAGATAATTGGAATTTCCTTAATTGACTTGAGAATCAAGATAGAAAGCACTACAAGAACAAGCACAATTACGAGCATAAAAACAAGTAGTCACCAGATATTACTAGATGAGGAGAAATCTCTTGAAATCTGCTGAGTAATTCCTGCCACGATAGAAGCAAAGATAAGCATTGAAATTCCATTTGAGATTCCTTTTTCTGTAATTAACTCTCCAATCCGCATAAGAAGAATTGAACCAACCGCCATAGCAAATCCAGACAAAAGCACTGTTGTAATATCTGTTGAGATTACTGCTCCTCCAAGCATATTATTCACAAGATATACGGATCCGATACCCTGTAAAAAAGCCAATGGTAAGGTTAGATATCTTGTATATTGGTTGATCTTTGCCTGGCCCACTTCTCCTTGCTCTGAAAGCTCTTCAACAGCAGGGACAATCACCCCAAGAAGCTGCATAATAATAGATGCCGTAATATATGGAGAGATTCCAATCGCAATAAAGGCAAACTTATCTAAAGCACCTCCAAGGAGCATCACAAAGTATCAAAATTCAGAACCCGCATTGAGAGTTTGGCTCATCAAAGCGGTGATATCAGCAAATGGAATAGGAATAAAGACTAATAGTCTGTATATCGCCAACATTCCAATGGTAAACAGAATTTTATTTCTGATTTTTCTGTTTCCAAAAACTTCACGAACTTTCTTATTAAAGGTTTTTAATGCTTTCATTGCATTTCATAAATAAAGGTAAATATAACTGATTAATAAAGATTCTTGGATCCTTTTCAATAGCAATCTTTATTAACGTCAATAAAGAATAAAACGCGAGGTCGGTCGCGTTTTATCAAGAAAAAAAGTTCTTATTTTATGCTTCCTCCTGCTTTTTCAATCTTTGACTTAGCAGTAGCTGAATACTGTTCAATTCCTTCAAAAGACAAAGCTTTTTCAAGATCTCCAGTTCCAAGCACCTTTACTGCAACCTCAGCAGAACGAATGTATCCAAGCTCCCAAAGGAGTTCTTTAGTCACAACTGCTGTAATTCTTTCATCCTTCTGAAGAGAGCCAAGATTTACATTTTGAATTGGAGTAATAAGCTTGTAATATCTCTTGAATCCTCTATGCTTAGGCATTCTCTGAACAATAGATGTCTGACCTCCTTCAAAGAATGGTTTCATAGAGTGTCCACTTCTTGATTTTTGACCATTAGCACCTCTTCCTGAAGTATTTCCTCTTCCTGAAGCATTTCCTCTTCCTCTTCTTCTGAGTTTATCGGTAAGTCACTTACTTCTCACTAAGCTGTGAAGTTTCATTATTATAACACATTTATACAGTTAAAAGCTATTAGTCTTCTTTTTTCTCTGTCTTTTTTGGAGCAGCCTTCTTTGCTGGAGCCTTTTTTACTGCTGCCTTCTTTGCTGGAGCTTCTCCAGCTTCAGCAACCTCTTCTTTTTTTGAGGCAGTTGCTTTAGGAGCAGACTCAACAGTTGGCTCAGATTTTTCTTCTTTTGTAGAAGATTCTTCCTCTACTTTTGCAACTTTAAGGGAAGAAAAGTAATCCGCATGCTTATATGCTGATAAGGCTCTAATAGTAGCTAAAGCATTATTAAGCTTGTTGTTTGACCCAACCATCTTAGAAAGGATATTTGAATATCCAGCAAGTTCCAAAACAGATCTGATAGATGAACCTGCCTTAAGTCCAGTACCCTCTGATGCTGGCAAAAGCTTAACTACACATGCCTTATATTTCAAAGCGATTGGATAAGGAACGGTATCATTTTTTGTCAAAGGCACTTCAAAGAGATTTTTATATGCCTCGTGAGAAGCTTTTTGAACTGCACTCGCTACATCATTACCCTTTGAAATTCCAAGTCAGATTTTTCCTTTTTTGTTTCCAACTAAGATTGTTGCTCTGAAAGAAAGCCTTCTCCCTCCAGTAGTAACTCTTGTTACTCTTCTTACCTCCAAAAGAAGCTCTTCAAACTCCTTCTTTGGCTTTTCTCTCCTTACATCTCTCTTATTGTCCTTTTCTGATCTCATAGACATCATACATTATACAGATAAATACAATATAGTCACTTAAAACTGAATACCTCCTTTTCTAAGTCCATCAGCGAAAGCTTTAATCCTCCCATGATAGAGGAATCCATTCCTATCAAAAGTCAGCTTTTCAACCTTTTTTTCTTTAAGCACTGCAGCAAATGCTTCACCTGCGGCAGCAGCTCTTTCAGTTTTAGTCGCTCCAGAAGCAGCTTTGTCTGTAGTTGTTGCAATAACAACTCCGTCAGCTCCTAATACCTGAGCTTTTACATAAAGATTAGACCTCTGAATCAAAAGTCTGAATTCAGGATTCAATGCTTTGATTTTAGCATTTACCTTATTCTTCCTTCTCAAGTATTTGCTAATCTTTTCTTGGAGTTTATTCTTCAAATAACTCATTTGTATACCGAAATAACAATCTAAAACTGAACACTATTATTTTTTCGCAGATTTACCTGGTTTAATCTTCACAAACTCATCAAAGTATCTAATCCCCTTTCCTTTATAAGGTTCAGGCTTTTTCATACTTCTAACCTTTGCAGCCACCTCACCAACTAATTCTTTACTAATTCCTGAGATAAAAACTACAGTGTTACCTTTTGGATCTTGTTCTGTCCTTATTTCAATCCTCTCTGGAATTGGAAAGTTTACCTTGTGAGCATATCCAAGAGAAAGCACGAGTGTTTTTCCTTCAACTTTTGCTGAGTATCCTACTCCAATGATAAGCAGTTTCTTTTCATATCCGTTAGAAACTCCTTCAACCATATTAGCAACAAGTGTCCTTGCTAATCCCCAAAGATTCTTATACTGGTCATCAAGAATAGAAAAAACTAATTCTGATTCATTTTTTTCAATTTTCACTCCGTATGGCAAATTAAGATCCAATGTTCCTTTCGGTCCTTTTACCTGTACCGCTGTTTCCTTCAAGTCTACTTCTACTCCCTGAGGGATTACAAGAGGCTTCTTACCAATTTTTGACATTTAGATAGCAACTTAAATAGTAAAGCACAATATAGCACAAAGACTAATAGATTTCTGCAATCAATTCTCCACCAAGCTTTTTTGCTTTAGCAACATGCACCGGCAAAAGCCCCTGACTTGTTGAAATAATTCCAAGTCCCTTCCCTCCAGCAACAGTTTTTAAATCCTTGTATCCAACATACCAAGGCCTAGATGGCTTAGAGAAAAACTTTACAGAAGGAATATCATTTACAGGATCTACTACTTTTTTTAACTGAAGAGTAATAAATTTCTTTCCATTCACTTCCTGCACTTCAAAATCCTTCACAAACCCATATTGTTTCAAGAGCTCAAGCACATTTACCTTAAACTTCGAGAAAACCACTCCATCTACGGTTGTTTTTCTCGCCATATATGCATTTTTGATCCTGATTAACAAATCGTGAACTGGTGCATTAACATAACTCATCTTAGTGATACGATTCATAAAATAAAATTAAACAATTACCAACTTGCTTTTCTAAGTCCCATAATTAAACCTTCTCTCGCATACCTTCTAAGGCATACTCTACAAATTCCGAAATCTCTAATATATGCCTTGGACCTTCCACAAAGTCTGCATCTGTTATAATATTTTGTAGGCTGTTCAAGATTTTCCCCTTTCACGAACCTCTTTTCAAACATTTTTCATTGTTTAGCTTTTAATGCTGTTCTTGCCATTGGTTATTTCACAATAAAGATAAATACCATATTATTTGAAGATAAATCCTAGCTCCTGAAGTAATGCTTGAGTTCCTTCTGGATTTCCTGCTGTACTTACAACTGTAAGCTGAATTCCCATAGGAAGTGTTACATCATCAACACCAAGTTCAGGAAAGATATTATAATTCTGAAGTCCAAGATTAAGATTTCCCTTTGGATCAAAGTTCTTCTTGGATATTCCAGAAAAGTCTCTTACCCTGGGAAGCACCAGCTTACAGAATCTATCTAAGAAATCATAAGCTCTTTCTCCTCTTAAGGTCACTTTTAACATTACTGGCATTCCTTCCCTGAGTTTGAAGTTTGAAATAGCCTTTCTCGATTTTATCAATACTGGCTTTTGACCTGTAATTCTAATCAGATTTCTTTCAAATTCTTCAAAATCTTTATGTCCTTTTCTTGTCACCAAAGATCCAATTCCCATAGCAACAATAACTTTATCTACTTTTGGAGCCTGATTGATGTTTTTAAGGTTCAAATGATCCTTAAGCTTTGCAAACAACTCAGATTTTTTCATAGCTAAACCATCAAAAATATTTAAAATGACTAATCAATAACTTTTCCAGTAACTTTCGCCTTTCTCACCTTCTTACCATCTTTTGTAAGTTCAATTCCAATTTTTGTAGCTTTCTTCTGATCAGCAAGATAATATGCGACATTAGAAACATGGAGTGGCAAGGTCTTTTTTATAAACCCCTGTCCCTTTACTGCTTTTTTTACAACATTTACATCCTTTACGGTTACCATATCTCCATCTAGAGCTTCTATTACAGAAACACTACCTTTTGCCTTTCCTGCAATCACGATAACTGGATCACCTTTTCTCAATTTTTTCATCAGAATATCTCGTTACAAATAAAGTTTATACCCTAATTACAATACTTCTTCTGCCATATTTGTAATTGGTCTATATTTTTCTCTCAGCTCTCTTGCTACAGGTCCAAAAACCCTCTTACCAATAGGATTCATATTTCCTTTCGCATCTTTAGTCAAAAGAACTACTGCATTATCAGCAAATCTAATATAAGTACCATCTTTTCTAGCAATCTCTTTCTTTGTTCTTACTACCACTGCCTGAACTACTTGCCCCTTTTTCACTGATGCAGTAGGTGCAGCATCTTTGATAGCAACAACAACTCTATCTCCAACATGTGCTATTTTTCCGTAAGAACCCTTCAATACTCTGATAATTTTTGCCTTTTTTGCTTGGGTGTTATCAGCTACCACAACCAACGATTCTTGTTGCAACATCGTTCAAAACCAAAACTAATTAAAACTATAAGATCTCAATAATATTCCATCTCTTGAGTCTAGAAATTGGTTTTACCAATCTCAATTTTACAGTATCTCCAAGAACTGCTTTATTCCCTGAGTTGTGAGCATAGTACTTTTTTCTCACTACAAATCTCTTCTTGTAGATAGGATGCATTTTTACAGACTTCACCAAAACAACACAAGTCTGATCCATTTTATCACTTACTACCTCACCAACGAGTTCTTTGATATTTTTTTCACCGATAATTGTCATTATGCATTCACTTTAGAGTGTAAAACGGTATTGATCCTAGCAATCTTAACTCTAAGATCTCCTATTTTATGAGTTTCCTTAAGAGCCCTTACACTATTTTTCATTCTGAGTTCAAAAAGTTCGTGTCTAGCTTGTTTTCTCATAGCTATAAGTTCCTTGATATCCTTTCCAGCAAGCTCTTTCATCAATAAACCAGTTCCTTTCATTACAATAACTTCTTTAGAAGTATAAAAATGTTACTTAATTTCACCCTTAGCTACAAATCTTGCTTTAATTGGCAATTTTTTCGCAGCACTTACAATGACTTTTTCTGCCAATTCTCTTGAAAGTCCAGAAAGTTCAAAGAGAACCTTTCCTTTCCTCACTGGAGCAGCATAGATATCTACATCTCCTTTTCCAGATCCCATCGGCATCTCAAGTCCTTTCTTAGTAATTGGAACATCAGGAAACACCCTCATCCAAAGCTGACCAACCTTTCTTGTTTCTCTTACAAGAACCTTTCTCGCTGCTTCCAACTGCTTATTGGTCACATATCCACTGGTTGTTGCTTTGAGTCCATACTCTCAGAAAGAGATACCAGTTCCTCTCGAACTTTTTCCCTTGAGAGAAGGAACAATCTGCTTTCTATATTTCCATTTCTTTGGAGTCAATAACATGATAATAACAACTGATCAATTAAAACCGAAATCTTGAGCATACTCTTAATCTAGATTCAGAGTTTTCTTCCCTTTTTTCTTTGCTGAAGGAATAACACCTTTTTCAATCCAAACCTTAATTCCAAGCACTCCATACTTTGTCATAGCCTGGAGGTAATGATAGTCAATATCTGACCTGAAAGTCTGAAGAGATACTCTACCGTCAATAAACTTTTCACTTCTTGCAATATCTGCACCTCCAAGTCTTCATCCAATCTGGATTTTAATTCCATTTGCACCCTTTTCCATCACCTTTGCAAGCACATTTTTTACTACTTTTCTATAAGGCATTCTCGCCTCAAGCTGAGAAGCAACATATTCAGCCATAATCTTTGCAGAAAGTTCAGGAGTTCTTACTTCTTTTACATTAATTTTGAATGGCTTTCCAAATTTTTTACTAAGATCTTCTTCTAACTTCTTAATTCTTTCTCCATTTTTCCCCATAAGAACACCAACTTTTGAAGCGAAAATAATAATCTCTCCTTCTTTAAGTGTCTTTCTAATCACAATCTTAGCCACCCCAGATCTTGCGAGTTGTTTTTCAACGAAATCTCTGATTTGGATATCCTCAACAAAAAAGTCAGCATTCATTTTTCTTGTCTTAGCAAACCATTCTGATGGTCGTGATTTCATAATCCCAACTCTCATTCCAACTGGATTCACCTTTTGTCACATCCTCACAAACTACTTAGTATTTAAAACAACTTTCACATATGCCCTATATTTTTCATAATGGCTAATCCTAGACCTAGAAACAAACCTTACCCTTTTAATCTTAGGTCCCCTCCCCACCTCAATCCTTTCAATAAAGAGCTGATCAAGTGGAAGTTTATTATTATTCACAGCATTAGCCGCCGCTGATTTAATAAGTTTATGAAGCACTCTTGCACCTTTTTTTGGCATAAATTCAAGCATATCCAATGCAGCAGTCACTCTTTTTCCTCTCACAAGATCCGCAATGAGATTAAGCTTCCTATCGGAAAGAAGTGCATATTTTAATGTTGCTGTAACCTGATTACTCATAACAATATAATTTTACACAAGTAAAGTAAATAGTTTCAAAATCTGAAGCTTTACCTCAGATAGAGAGCTTAGAACGGATGTCCTCTAAAAGTTCTTGTAGGAACAAACTCTCCCAATCTATGTCCTAACATATCTTCAGTCACATACACACTTACAAATTGTTTTCCATTATGCACAGCAAAGGTATATCCAATAAATTCAGGAACAATATGACAAGCCCTATCCCAAACTTTAATCACTTTCTTATCTCAGGTTTCATTCATTTTCAAAACCTTATTCATAAGTTTTTCATTCACATAGAAACCTTTTTTCAGAGATCTAGCCATTTAACAAAACATATCTAAATAAAATGGAATTGAAGATTATTTTCTCTTTGAAACGATAAATTTATCTGACCACTTCTTTCCCTTTCTTGTTCTCATTCCAGCAGAAACAAGCTTTCCACTAAATGACTTAGGCCCTCTCTTAGAACCAATAGGGGAGTGGGCTTCTCAACCTCCAAGCGGATGGTCTACAGGGTTCATATTCTTTCCAAGCACTCTTGGCTTTCTACCAAGCCACCTTTGCCTTCCAGCTTTACCGATTTCAACATTCTTATGATCAGCATTAGAAACCTCACCAAGTGTTGCCCAGCAGTTTTCATTGAACTTTCTCACTTCTCCTGAAGGCATCTTCACAAATACAACCTTTAAGGCCTCATCTCTACCTGCTAAAGTAGCAAATCCTCCAGCAGACTTAATCAACTTCCCCTCAGAGAAAGGAGTCACCTCAAGATTGAAGATTGCCATTCCTTCTGGAATATCCTTCAGCTGCTTTCTATTCCCAGGAGCGATTTCTCCTTTCACACCATTCATTACCACCTGACCAACTTTTGCACCTTTCCAAGCCAAAACATATCTCTTTTCCCCATCAATATAATGCAAAAGAACAATTCTAGCTGTCCTATAAGGATCATACTCTACAGCAGCAACTTTTGCGGGAATATCTATTTTATTATAGCCCTTAAAATCTATGATTCTATATAATCTCTTATGTCACCCTGCCATAAACCTAGAAGTTATCCTTCCTTGGTTATTTCTTCCTCATGTCCTTCCAATCATCATAGTTAATGATTTTTCAGGTCTTTTCTTAGTAAGCTCTGAAAAGTCATACCCAATCATAAATCTTCTGGAAGGCGTATATGGTTTATACTTCTTTACAGCCATCTGAACAAAACATTATAATTACTAAAGAAAACAATTACACAGCAAAATCAATCTTTTCCTTTTCTCCCAAAGTCACTATCGCTTTTTTAAAAGCCCTCTTTACAAGCTTTCTTCTTTCTCTTCCTTTAAATGCTGTATTTACAACATTAATCTTTAGAGGAGTCACCTTATAAAGATATTGAATAGCAGCATCAATATCATTTTTATTAGCATCACTATGTACCTTGAAAACATATTTATTAGCAACCTCTTGCTGTTTATAGGTCTTCTCAGTTAATACAGGAGCAAGGATAATATCGTAGGGACTAAATTTTACGAGAGCCTTTTGTGCAACACTCTTTCTCGCCCTTCTCTGCAATTTCTCTTTAAATGTCATACTGTTGTAAACAAAAACATAAATAATAAACTAATTCTGATTTACAAATTCAAGTGCCGGTTTCAAAAACACAATAGCCTCAGCGTTCATTAGATCATAAGGATTAAGATAATCCACCAAAAGGTATTTCACCCCCTCAATATTTCTAAAAGACTTTACAACCCCATCTTCTTTTTTATCTAGAACGAAGAGCACTTTTTTACCCCCATTCCCCATTTTAGAAAGAAGCGCTATAGCAGCCTTTGTATTCGGCTCCATAGTATCAAGATCCAATCCTGAAACCACTCACTCCTTCACTTTCAAAGTCAAAAGACCATTAAGAGCCAGCTTTCTTGCCTTTTTAGTCATTATCTTTTCAAAATTCCTCTCTGCTGTTGGACCAAAAGCAACTCCACCATGCCTTCTTATTGGAGAATTTTTATCCCCCACTCTTGCATTTCCAGTCCCTTTCTGTTTGTAAAGCTTTCTTCCAGATCCAGCAACTTCAGCTCTTGTTTTTGTAGAGGCAATTACATTACGAGCGTTCGCCTGCTGAAGGAGAAGATATTCATGAATAAGAGAAGGGCTTACAATCTCATCAGAAAAGAGCTCGGGATTCAAATCAACTTTTGAAGCAAGCTTCCCATTTTTATCATATACATCAACTGCATACACCATCTTTTAGCTATCTATAAGGTCATAAAAATTATTCTACAAAACATTTCAACTTTCCGTTTCTTGCACCAGGCAAAGATCCTTTAACAATCAAAAACTTCACGCCATCCTTTTCTACGATATTCAAAAGAGGAATTCTTTTGAGAGTAAGCTGCTCTGAACCCATATGTCCTGCATGAGGATGCCCCTTCATAGTTCTTCTTGGCTTTCTATTACCCTTAGAACCACCTGCTCTAGAAAACTTGTGCCCATGGGTAGCAGGACCTCCTTTAACATTACACCTCTTCACTAGTCCCTGATACCCCTTTCCTTTAGTCATCCCAGTCACAGCAAGAGAAGATACACCTTCCAACAAAGAAGCGTCAAGAATCTTTCCAGCCTCGTTCGCAGAAACAAAAGCCTCATCTACAGGAAACTCTGTCACCATACTATATATCAGCTTCTGCCCCTTTTCTTTATTGAGTTCTTTTTTTTCAACCCCAACAACTACAGCAGAATACCCATCTTTTTCCTGAGTTTTATATCTAATAATTTCTTGTGGAAGAACCTTAGCAAGAGTAACCGCCACAAATTTATCACCAATCCACATCTTAGTCATCTCTTGTTTTGCAACCACAACTCCTCCTTTATTTGCCATACTAACACATTCTGATCAATTAAAACAAAGCACAAATTACCAATTACGAATAGATTTTATATCCACAATCCGTAATTCGTATTTTATAATCCTCTAAGCCTAGAAAACCTTCACATCTACAAGCACTCCTACGGGAATTGAAAGATTCTGAAGATACTCCATTGTCTTCCCTCCAGTCTCTACAACATCCAAGACTCTTGTATAAGAGATTCTTTCAAATTGCTCTCTTGCTTTTTTAAACTTAAAGTTTGATCTTAACACGGTATATACCTTCCTTTTCTTTGGCAAAGGAATTGGTCCTTTAATCTGAGCACCTGATTTTACCAAAAGTCCTACAACCTTAGAAACAGAAGCTTCCAACATTCTTACATCATAACTCTTTAACTTAATTCTCAACTTTGGAGTCTGATCTTTTTTTACAGCCGACATATAACCCTTTTGATAGTAAAAATAAATATAAGAAAACAGACCCCAGAATTCAGTCTTAAACCTTTTCCAAGGTCTGCATCTCTATAGTAAATTATTCAAGTACTTTAGTAACAACTCCAGCACCAACCGTTCTACCTCCTTCTCTAATTGCAAATCTCAAACCCTCTTCCATAGCTACAGGATAGATAAGTTCAACAATAATCTTTGCATTATCACCAGGCATAATCATTTCTACACCAGGAGCAAGTTCAATATTTCCGGTAACATCCGTTGTTCTCATGAAGAATTGAGGCCTATATCCACTCATAAATGGAGTATGTCTTCCACCTTCTTCCTTTTTAAGTACATACACTTCAGCTTCAAACTTTTTGAAAGTCTTCACAGTACCTGGCTTACAAAGCACCTGTCCTCTTTCAATAGCATCCTTGTCTACACCTCTAAGCAAAAGCCCAACATTCATACCAGACTCTGCCTGATCCCACGCCTTGTGGAACATTTCAATACCTGTAACAACAGTTTTCAAAGGATCAGCACCAAGACCAAGAAGTTCAAGCTCATCATTCATTTTTACAATACCTCTTTCAATTCTACCTGTTGCCACAGTTCCTCTACCTTTAATAGAGAACACATCTTCAACTGGCATCAAGAAAGGCTTATCATTATCTCTTTCAGGAACTGGAATGTAAGAATCCAAAGCATCAAACAACTCCCAAATAGGTTTTGCAGCAGCCTCATCAGTAGGATTTTCAACAGCCTTCAAAGCTGATCCTCTAATAATAGGACAATTTTCATCAAAACCATTCTTCTTTAGAATCTCTCTGATTTCTTCTTCTACAAGATCAATCATATCAGGATCATCTACCATATCACATTTATTCAAGAAAACAACAATCTTTGGCACATTAACTTGCTTAGCCAAAAGAATATGTTCCCTAGTCTGAGGCATAGGACCATCAACAGCAGAACAAAGAAGAATAGCTCCATCCATCTGAGCAGCACCTACGATCATGTTCTTTACGTAGTCAGCATGCCCTGGACAGTCAATATGAGCATAGTGTCTGTTGTCAGTTTCATATTCTACATGTCTAGAAGCAATAGTAATACCTCTTGCCTTCTCTTCTGGAGCGTTATCAATTTGATCATACGCTGTAAATTTAGCATACCCTTTTGTAGCAGCAACAGCTGTAAGAGCAGCAGTCAATGTTGTCTTACCGTGATCAACGTGACCAATAGTTCCAACATTTACGTGTGGTTTAGTAGCCATAGTAATACTGTTTAATAAACTAAAAGTTAAACATAACTAACTATATAACAATTTCCTTTATAATTGCAAGTAATTCTTCAAAAAAAAAGTTGATCTTGATCATTTGGCAGACCTCACTAGAAAATAGCAAGCCACCAACCCTATTTCAAAACCACCCTCCAAATATTTAACCAAATCAAGTAAGGCAATATATCCAGAGAACCTTTTTATTTTGTAAGATATTAACTTTTTAACCTCGGGAATGCGGGACTCGAACTCGCGACCTCTTGGTCCCAAACCAAGCGCTCTAGCCATCTGAGCTAATTCCCGAGAAAGAATGGTGGGCGTGGGTGGACTCGAACCACCGACCTCACCCTTATCAGAGGTGCGCTCTAACCGCCTGAGCTACACGCCCATTCTTTTCTCTATAATTTTTAGTGGTGCCAAGAGGCAGAATCGAACTGCCGACACAAGGCTTTTCAGGCCTCTGCTCTACCACTGAGCTACCTTGGCTTTTCCTTTTGATGTGCATCAGAGATATTTCTCTTTTCTACCTATTCATATTTCTTCTTTTATGCTTCAGTAGAAATAGAACAGGTGTCTTTATAGTAATTTACTTATTAATTTCAAGAGTATTTTTATGACAAAAAAGCAATGGAACAGACATAGGGATAGAAGCATAAAATTCAGAAAACATGCAGCGTTTTGATGTTTTACCTTTCTGAAGATAAACCTATCACTACAGTCATAAGATTGTAAACAAAACATTAGAATCCATCACCTAAAATTATAATATCGTAAAATAACTATCCCAGGAAAAATAACAATCTTCTCTTATTTTTCAAGGGTAAGTTTATCTCTCTCCACTTATCGTGAAAAGAAACTCTAAAATAATCTCAACCTAGCTAAATTCTTAAAAAAGAAAAAACTGACTTGGGAGTCAGCTTTCTTTAATTCAATAGTACTGAAAAGAGATAAAGTAATGGACAAAGTGTATAATTTTAAGTGTTTGATTAGAATGGTGGTCTATCCCCACCTTCCGGTAGGGATGCCTTGTTACGACTTAACCCCAGTCACTGATTTCCTCCTTAACCCATATAATGAGTCTTCAAAGAACCTCAGCTCCCATGGTTTGACGGGCGGTGAGTGCAAGGAACAGGGACATATTCACGGCAGCATGGCTGATCTGCCATTACTAGCAATTCCAACTTCATGTAGTCGAGTTCCAGACTACAATCCGAACTAGGGGTAGTTTTAAAGATTTGCTCCACCTCGCGGTCTTGCTTCTCGCTGTACCTACCCATTGTATTATCTATGAGGCCCTAGGTGTAAGAGGCATGAGGATCTGACGTCATCCTCTCCTTCCTCTCGGTTACCCGAGCAGTCTGCTTAGACATACTAACTAAGCATGAGGGTTGCGCTCGTTTCCAGACTTAACTAGACACCTTGCGGCACGAGCTGACGACGACCATGCACCATCTGTGTAAAGCTCCGTATTGCTACGGATATGCGACTTTCACCGCAGACACAATACATGTCAATCCTAGGTAAGATTCTTGGCTTATTATCCAATTAATCTAGATAATCCACTGCTTGTGTGTTCCCCCGCCTATCCCTTTGAGTTTCATTCTTGCGAATATACTACCCAGGCGACTTGCTTAACTCGTTAGATTACGACACTCCTGCCAATAAATTGACAAAAACATCTAGCAAGCATAATTTACGGTTCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCGAACTGTCGTCCCTCATCGTCAAGTATTCTGTAGTTAGCTGCTTTCGCTTTCGGCGTTCCTTCCGATATCAACGCATTTCACCGCTCCACCGGAAATTCCACTAACCCCCAGATACTTCCAGAATTGCAGTATCCACTCCCTTTCTACGATTGAGTCGTAGGATTTGAAAACAGACTTACAATTCAGACTACGGACACTTTACGCCCAGTAAATCCGGACAACGCTTGAGACCTACGTATTACCGCGGCTGCTGGCACGTAGTTAGCCGTCTCTTATTCTTGACATACCGTCATTCGTCTTCTGCCAAAAAAGAAGTTTACACACCGTAATGCTTCATCCTTCACGCGAAATTGCTGCATCAGACTTTCGTCCATTGTGCAAGATTCCCCACTGCTGCCTCCCGTAGGAGTATGGGCCGTATCTCAGTCCCATCGCGACCGGACATCATCTCAGACCGGTTACCCGTCATAGGCTTGGTAAGCCATTACCTTACCAACTACCTGATAGGACGCACCACTCTCCCGAAGCGATAAATCTTTCCTCACATTGGAATTTCACCATCGTGAGCATATTGGGAATTAGCTACATCTCTGTAGTTATACCCATCTTCGGGGCAAGTTAAGCACGCGTTACTCGCTCGTTTGCCACTAATCTTACTACGTATTGCTACGACGCAAGATCCGTTCGACTTGCATGTGTTAGGCATTTCGCTAGCGTTCATCCTGAGCCAGGGTCAAACTCTTCAAAAAGTTCCACACTTTATCTCTTTTTAGTACTATTAAAGATCTCTTCAGCTAAGGCGTTTCCATCCTTAGTAATGGTAGCACTAACCTACTTTCCCAGCCCTGATAGAACCAGTATCATCGACCAGTATATGCTTAACTTCTGTATTCGGAATGGGAACAGGTGTTTCCATATACCGCATCAGCACTACCGTTAATAAAGATGAATCAGACTAATAACGATTTTCATACAAAAATCAAGTAAAAATCTGATTTTTTATCCTTTTAGTATCCTTACAATACCATAATATATTACCTATTTCTTAGAAAAAAGATAAACCCGCTCGACCAATTAGTACATCTTAGCTCAATCCCTTACAGGACTTACACCTAATGCCTATTAAACTGGTAGTCTACCAGCGGTCTTACCTTCATAAAGAAGAGGGATATCTAATCTTGAGACAAGTTTCCCACTTAGATGCTTTCAGCGGTTACCTTTTCCGAGCTTAACTATCCAGCGGTGCTCCTGGCGGAACAACTGGTACTATCAGAGGCTCGTCCAACCCGGTCCTCTCGTACTAGGGTCAGCATCTCTCAAATATCCTACGGTCACAGTAGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAACTCGCGTCCCGCTTTAATGGGTGAACAACCCAACCCTTGGGACCTTCTACAGCCCCAGGATGCGACGAGTCGACATCGAGGTGCCAAACGGAATCGTCGCTGTGAACGCTTAGATTCCATTAGCCTGTTATCCCCGGAGTAACTTTTATCCGTTGAGCGGATCCCCTTCCACTTGGAAGATCCGGATCACTTTCGCCTACTTTCGTATCTGCTCGACCTGTCAGTCTCACAGTCAAGCTTGCTTATGCGAATACACATTCACCACGGTTTCCATCCGTAGTAAGCAAACCTTTGCACGCCTCCGCTACTTTTTAGGAGGCACCCGCCCCAGGTAAACTGTCCAACTGAAACTGTCTCCCAACCTAGGGATTAGAATTGCTACATATCTTGGGTGGTATCTCACTATTGGCTCCGCCCCAACCGAAGTTGAAACATCAAAGCCTCCCACCTATACTGAGCAAGACATGCTACAATCCAATATCAATCTACAGTAAAGCTTCACAGGGTCTTTCCGTCTAACTGTGACTACCCGGCATTTTTACCGGGAATGTAATTTCACCGAGTTTAAGGGTAAGACAGCATGGGGATGATTATGCCATTCATGCGCGTCAGAACTTACCTGACAAGGAATTTCGCTACCTTAGGACCGTCATAGTTACGGCCGTCATTCACCAGTGCTTAACCCTCGAGCTTGCACAAGAGAGCATAACATTCTGGCATTGGACAGGCATCACCCGTTATACATCCTCTTACGAGTTAGCAACGAGCTGTGTTTGTGATAAACAGTTCCCCCCATCTCTTTCGCTGCGATCCCGATTGCTCGGGACAGGACTTATTTCGAAAGTACGTCCGCTAATTTGCCAAGTTCCTTACCCATTAATTATCTCGATTATCTTAGTATTCTCTACCACCCCACAAGTGTCTGATCTGAGTACGGGTTACATAAAGATTAAGTTTAGAAGTTTTTCTCGGCACCAAAACGATGAGAATTTCTAATTCCGAGGAATCAAATTCCGATCAAGCTCAGTATCAATAGCTGTGGATTTTCCTGCAACTACCTTTATAACCTAGGGTTATAAAAACCTCACTCTTTCGACTCAAATCCTTAATGAGCTCTCACAAATCTCAATGCGTTACTCCATCACTCTTTATATAAGTACTGGAATATTAACCAGTTATCCATCGACTCTCCACTTCTGGATAAGCCTTAGGTCCCGACTAACCCTACGACGATGAACGTTGCGTAGGAATCCTTAGGGTTTCGATATATTGAATTTTCATCAATAAGCGTTACTCATGTCAGCATTTTTACTTCTTACTAGTCGACCACATCTCGCGATGTAGCTTCATCCTGTAAGAACACTCTCCTACCACACAGTGTATTACTACACTGGTCTTCGATTTCGGTATAATGCTTAGCCCCGTTACATCTTCAGCGCATCTTCGCTAGTCCAGTGAGCTGTTACGCTTTCTTTAAAGGATTGCTGCTTCTAGGCAAACCTCCTGGATGTATAAGTAAAGACACCTCTTTTTCCACTTAGCATTACTTTAGGGACCTTAATCAGAAGTCTGGACTGTTATCCTTTTGACTACGAAACTTGCTCCCGTAGTCCCACTCTGCAATATTCCTTGAAGCATTCGAAGTTTAGTAAGGTTTGGTAATGTCTCTCGACACCCCTAGCCTTTCCAGAGCTCTACCTCTTCAAGATCTCAAAATGCAGGCTGCACCTAAATGCATTTCGGAGAGAACCAGCTATCACGGTATTCGATGAGCTTTTCACTCCTAAACACAGGTCATCGGAAGGTTTTGCAGCACCAACCCGTTCGGTCCTCCAGCATAGTTTCCTATGCCTTCAACCTGCCCATGTCTAGCTCATACCGCTTCGGGTCTATTAATAATAACTAAAGCGCCCTATTCAGACTCGGTTTCCCTTCGGCTCCGCCTATAACTGGCTTAACCTCGCTACTACTAATAACTCGCTGACCCATTCGTCAATAGGTACGTGGTCACCTTGCGGCTCCCACTCAATTGTAAGCAATAAATTTCAGGTTCTATTTCACTCCCCTACCAGGGGTTCTTTTCACCGTTCCCTCACGGTACTGTTCGCTGTCGGTGTAAAAAAGTATTTAGCCTTACCCAGTGGTCTGGGCAGATTCATACCAGATTTCTCGTGTCTGATATTACTCAGGTGTCGTCACGATGTGGTGTCATATTTTCAGATACGGGACTTTAACCTTCTATGGTGCATCATCCAAATGCTTCTCCTAATACAACATCTCTCCATCCAAAGAGTTCTAGCTCTTCCCGGACGATCCTACAACCCTCTTATTAGCAACGCCTAGAAGCTTACACTAATAGAGTTTGGGCTCTTCCCTGTTCGCTCGTCACTACTAAGGGAATCTCATTACGATTTCTTTTCCTTCGGTTACTGAGATATTTCACTTCACCGAGTATTGTCAACTTTGTATTCTCCTATAGAGAATAGGTTGCCCCATTCGGAAATTCTTGACTCCAAGCGATTGTTGGCATCTCATCAAGACTTATCGCAGCCAGCTACGTCCTTCTTCCTCTTTTTTACCCAAGGCATCCTTTTATTGCTTTTAAATAGATTTATCTACTAAGAAATAGTGTAATATATTATGCTATTGTTAAGGATCACTTCAGCAACCTCTTCGCTTTTGGTTGCGAGCATTATATTAAATATCTTCTATCTAAATGCAAGAGATTTTAAAGATTTTTTTTAACAAGGTAATATTTGGCATTTTTCTCCTTTTTGGGTCATTTCGTTTTTTAATTTCTGAAGATTGATTAAAAAAATCGACCAAAAATTCAAAAATCTGATTTTGTCTCTTATTTTAATTTTTCTTCCGGTCTTATGCGAACTTTCCCACATTAAAAGACTCCACTTGTGGAAGACTACAAAAAAGCAACCTTTTATGCTCTAAAGGTTTGATCATTTTTTGATACCCCAATACCATTTCTTCATCAAGAATCTGAATTTATAGCTGATTTACTATGGTATAGCTTTGGATAAATAAGCGAAACTACCACAATACGACAGTTTTCTATATTTCTGAGTTGATTTTCGGAAGGGAAAGTATATCTAAGAAATAAACAATTTTACTTTTTATTGAAGTTATGGAAGCTGCTTTAATCTTCTTTGGATTTATTAATGTCGTCTCCTTTACTATCCGATGAATTGACAAATGGAAGGCAAAAAATAAAAAATGGAGAATTAGCGAAAAAACACTTCTTATCTTAAGCATCTGCTGATGATGGCTAGGAGCGATTCTTGCGATTGACGTCTTTAGACATAAGACTGTAAAGTCAAGCTTCCTTTGGAAATTTTATCTTATTGGCGGATTTTGGATTGCGATCCTTTTTGGAGTCCTTTATTTTTGGTAAAATTTCACACCGCATTTTACCACGATTTTGACAGCGGAAAAAGAGTCTGATTTAGTTATAGAGGACGTTTTAGACGATGCTTTGAGGCAGGATATTTGATTCTCACTTGTAAATCGCTGGTAATTCCCTACACTTGAAGTATTAATTCAGATTTATTTTTTTGGAAGAACAGAGATGAAAATTGGAATTGTTGGATTACCAAATGTTGGGAAGTCTACCTTGTTTAATGCTTTAACAAAGTCCTATGCTGCTCCTGCTGAGAATTTCCCCTTCTGCACTATAGAACCAAATATTGGGATTGTAGACGTTAAAGACCCTAGAGTTGAAGCTCTGGCAGAGATGTCTAAAAGCCAAAAAATTGTCTACGCTAATACCGAATTTGTGGACATTGCCTGACTTGTAAAAGGAGCAAGTAAAGGTGAGGGACTAGGAAACAAGTTTCTTTCCCATATTAGAGAAGTTGACACAATTGTGCAAGTGCTAAGATATTTTAAGGACAGTGATGTCGTCCATGTGGAAGGCGGAGTTGACCCTCTAAGAGATAGAGAGATCATCAATACTGAACTTATTTTTTCCGATCTTGAACAAATAGAAAAAACACTTCCGAATCTTCAAAAGAAAGCAAAGCTGGCTCAAAATAAGGACGATAAAAAGTCCGCAGAAATCCTCGAAAAAATACAAAAAGTCCTCTTGGAAGGAAGACTGGCAAACACCTTGAGAGAAGAGTTTGCGGAGGAGGAGCTCAAATTGATTAGGTCCTATAATTTTCTGACCCTAAAACCTTTTGTCTATGCACTGAATATTTCTCAGGAGGATATTCAAAATGCTGAAGTTCTGAAGAAGGAATTTGAAGAAAAACTCCAAGCTCCTGTTGCGATCGTCTGTGTCAAGATTGAATCCGAGATGATGGAACTTTCCAATGAAGAAAAATCTGAATTTCTGACTGAGCTTTTGGAACTCCAACAGGGAGCCAAGATTCCCACTCTGGATGATTTAATTTCCCTCGCCTTCAAACAGCTCGGACTTATGTACTATTTCACGACAGGAGAGAAAGAAACTAAAGCTTGGACCATCCCACTTAATTCTACTGCACCGCAGGCAGCCTGAGCAATTCATTCAGATTTTGAAAGAGGATTTATTAAGGCGGAAGTAATCAACACAGAAAAGCTCTTGGAATCAGGTTCTTGGGCTAAGGCAAGAGAGAAAGGACTCCTACGTTTAGAGGGGAAAGATTATATTGTGCAGGATGGAGATGTCATGATCTTCAAGTTTAATGTCTAGAGAAGCTACGGATCGCATTCCTCTTAGAATGGGACGACTCTATTTGATGAGTTCTTAGAGCACTTCTTCTACTTCTTCAAGTTTTTTCTCTATAGAATAGTGGCCGAATAGTCTTATTGCGAGGAATACAACATAAGCAATAATAGTAAGAATCCACACCACCAATCTTACCACTCCGTAGAAGAGCAAATACATTGCAAAAACAGCCCCAACCTGGAATATTGGCTTTTGATAAACACGTTGTAGCTGCCCGAGTGTTGCCTTACAGATTTCAAAACTAATGCTTTGCTGAGTTTTCACCAGCCCTTCTAGAATAACACTCTTGATTTGTCCTCCCATCTCTTTTATAGGAGAAGATGTTTCTTGATCTACAAGACTTTCGGCTTCGGCTTCATTTTCTGGAGTATTAAAAATGTTCAGCTCAAAAGGATTTGTAGATTTTTGTATGATTTTTTTGGATCGTCAAGAGATCTGTTCACATTGGAAGGGGAACTGACTATTCATTCCAAGCACCGCAAATCAAAAGAGGATACTGGTTATTGTGGCAAAAATGTAACCACTACTGGTAAAATACACAATCCAATTAATCTTCCTTACTTTGGAGAAATAGCTTCCTAAAGAAAAAAGTAAAAATCAGATTGCAAACAGCGAAAGCCACATAGCTATCATTAATTGTCGTGGAGCATTTCGTATTATCAACTGAAGAACGGCTCCTAAAATAAGTATTGGAAGCCAGATTTTTATCTTTTTTACCCTCCTAAGATCTATAGCAAGGAATATTAAGCCAATAAAGAGGACACAAAAGGCTGCTACTGCCCAAAGAACCTGCCTTTCCCACAAAAGCTTTCGAAGGAAAAATCCATAGGTAATGAGAGAAAAAACTAAGAAAAAGCAGAGCCTTGGAAGGTGATAGCTTTTTTTGTGAGATGAAGATAGAGAAGAAGATATTGTTTCCATTATTTAGAATGAGAAAAGGGAAGATAAAGCGTTTTGAAAGAGAAGAATGAACACAACAGCTAAGATCATTGCGGGAAGAAAAGGAACCGACTGCTCGGTCATTTTTGGACTCAAAAAATTCGCTTTCTTTCTAAAGATTTTATTCTGAAGGATAAATCGAAAGATTCCAATACTTCCACTTAGGACGAAAAAAATCAGTATTATACCTGCTCCCTGTAAAACATCTCCACGATTTCCTATCCCTGCAAAAAGAAGGGTTCCTAGATAAGGCGCGATCAATACATCCCCCATTCCGATTCCTTCTTCATTATTGTGGTATTTAGCCTTCACTCGCCGTTTGGCTAGCCGATATAATAGCAGAAAAATAAGCAGAAAACTTATCCCGCCCCAAAGAGCCTGCCAAACGAACAATCAGCATCATAGTGCTAAAATCAAAACTCCACTTATAATAACAAGAAGGGGAAGATGCACTTCGTATCGTAATACATCGTAAATTAGAAGGAGCCAAAGCATTCGCCCGGTGATTGTCCAGAACACTACAGGTACCATACCTGCATCAGAAAGATAATACCATATGCTTCAGAAAATCAAAGCAGATCCAAGCTCTAGAATTGGATAAAGTCGTGAGATTTTCTTTTTGCAATAGCGACATCTTCATTTTTGGAGGAGGAAACTGATAACGGGGATAAGATCACAGGGCTGCAATCTTTTTTTACACTGAGGACACTCACTTCTTCCTCGTAAAATCGAACTAGCCTGCTCAAGACTCTCACTATCTCCTCGTCTGGAAAGAATTACTGAGCCAAAAGATCCAAAAACCAGACCAAGAAAGATTGCTATGATTAATCCAAAAGCCATTTCGCTTGTAGGTAAGTAAAAATTCAAAGTGAAGTATAGTTTTTTCTCCTACCTTTGCAACGGATTCAGGCTTTTTGAGCTTTTTTGAATATGGCTTTGGAAGAATTATATTATATGAGAAGAGATGAAATTAGGAAGCTTTTTGAGGGCTATAACTATTATGAGAATTATTCTTGTTTTTTTGGGGAAAATGTTTAGACTCTTTATGTTTTTATATTCTTATAGCAATAGTAAAATGACTAAACTAGAACATTATTCTGCATGCGACTGCCACTGCAGTGCCTGCGAAGACAATTATTGTGAAGAATGCACGTGTTGCGACTGTGGAGAAAGCTATGACTCCAACAACTGTGCAAGGATCGAAAGGATCGTTCCAAACTTTAAACTTACGATGTATAATCCTCATAGCCAAGAGATTGAAAACAAAACTGTAAAGGATTACAGAAACAAATGGCTTGTTTTGTTTTTCTATCCAGCTGATTTTACCTTCGTCTGCCCAACAGAACTTAAAGATCTCAACCAAAAAGCTGAGGAAATCCGTGCAATGGGAGATGTTGAAGTCTTTGGTGTGAGTGTAGATTCTGTTTTCTCTCACAAGTCTTGGCTTGAATCCGAAGAAATGTTAAAAGGCTTCCAGTTTAATATGATCAGCGATAGAAATACTGTCCTCTCAAGGTACTTTGGTATTCTCAATACTGCAACTGGAAACGCAGAAAGAGGAACCTTTATCATCTCTCCAGACGGTGTTCTTAAGACGATAGAAATCCACACTGATGCTGTTGGAAGAAGTGCAAAAGAACTTGTAAGGAAGCTCTATGGATTAAAGTTTGTAACGGAAAACAAATGACATGCGTGTCCAGCAAGCTGGGAGAACAATATGAAGACTATGCAACCAAACATTAAAAATGCTGGTCATGCAGGAGCAACCTTATCTTAACCATTAGACGATTAGGGAAAATCAGATTTTATCTTTTTGAATTTATTAGGATCATGAAAGGATATCACGCAAATATCGAAGAGCTTACACTAAAAAATGACTATTTTAGGCAAGTACTTTATACTGCAAAGGGACTTCAACTTGTGTTGATGAGTCTTCTTCCTGGAGAAGAGATCGGATTAGAAGTTCATCATGATAATGTGCAGTTTTTCAGATTTGAAGCAGGGGAGGGGAAAGTAATCATTGATGATAATGAGTATTTGGTACAGGACTGAAGCTCGATCATCGTCCCTGCAGGTGCACAGCATAATGTGATCAACACTTCTAGCACTGAACCATTAAAGATGTATACTATTTATTCTCCAGCGCACCACAAAGACGGCGTAGTTCACGCAACCAAGGAAATCGCTGAGCAAGCAGAACATGATGGAACCGATGAATTTGACGGTATAACTACCGAGTAAAGACAACTATCCAGTAAAAGAGTAGTATATAACAGTGAAAAAGACTGATCCTCTATTTTGGATCAGCTTTTTTCTTTTTTTCTGTTGAAAGAAGTGGCTATGAAATGAGCGGAATCAAAAGTCCGTTGCTGAGGCAACCTTTAGGATCGATCATTTCTAAAAGTGTAGCTAGAAAAACACTTGCATAACTCCCCGTTGGAAGAGAAAAATCTAAGAGGAGGTCTTTAGCTTCCCGTTTTCGGCTGAGGGAATGAGGATATGCTCGTAGGGGCCTCCTAAAGCCATAGAGATGATAGGTCTGAGAAATAGTATTTCCAAAGGTATCAAATCCGCTGACTTTCCTCTGTCGCTCATCATAATATCTTGCCGAACTGCCTTGGTTGCAAGTGAGCTGTTCTGTCCCCAACACTGCTCCCGTAGGAAATCGCGAATCTTGGTCAAAATCACTCGATTGAGTAATGACTCCTGGCTCGAGTACTGCAGCCCCTTGATTGACTCTTTTTAGTGCCCAATAGTCAAAATGCTGAAGCATTCCAGCGGAATAACTCGCTACTGCAGTTCAAAATGCGTTTCGTCCGTTGACCATAATATCTCAGTCTAAGATAAACGCTCATTCCTTTCGTCTTTTCATCACAAGTTCGTTAAACCACATACTCCCAAAGGCTTGAAGCATGAATTTTACTTGATAGCCGTGATCTTGGAGACGTCCCTCGGAAAAGATTTTTTTTGCTTTTTTAAAATTTTTGTTTCCTTTTCCAAATCTCTGGATTCAAAATGCATTTGGAAATCAGATTTTTTTACTTTTTTGAAGGTGTTGCTCTAGCTGTTCTCTTGCTTCTGGAGGAAGAGCTGCTCTTCCCCTTAGCCTAATAGTGAAGTGATTCCCTCCGTTCTTGCCGATCGCTAGAGGAGCGTGATGCCGTCAGGTCGCCAGGATTGCAACTTTTTGCTTTAAGCTATCAAAAAAAGTGGAGCTTCCTCCACAAGCATTTAGAGCCTTTTGGGAAATACTGAGCCGTTGTCTTGTAATTCAGGCTTTATCTTTTAGTCCTGCAATCCCAATATCCCCCCTTTTGAGCCCCAGCGTCTGGCATAGTGAGAGGATAACCTCCATGGTATTTACTAGTTTTTTCTCGAAGAAAACATAAAGGAACTCTCCCTTTCCGCTTAACTGAAAAGGTAAAAGCTCCTCTACAATAAAATCCTCTGGCTGAGTTTTGAAAGAAAAATACATACAGTCTTTTTTTAATTCGCTCAATATAAATGCCCAAGTTGGTTGAAAGAGAGCATTTCTCGTCTATCCTCTACAAAGCGACAACGGCTAAGTGAACAAGGAGATGTAACAAAACGCTCTTCTTGTCCTTCCTTTTTGAGCAAGGTTGCTATGATAGCACTATTTATGATAGGATTTCCCACAATGAGGATACGAGAAGAGCTTGGATACGTTTTGGTAAGCTCGCGAAGAAAGAGTTTGCAACGTTCACGCACCTGATCCCGTGTTTCTTCTATTGTCTGACTATGCTCTACGGTATCACTCTTGATGAAAAGGCGACTCAAAAGCTTTACTTGCTCTGGATTATTTCCTGTCGTATTTTCAAACGTTTCTCTAGTGAGTTTGGTGCAGATTTCTGCTCATTTTTCTCTAATTCTTGGATCAACGATGATCTGAAATCTATCATTTTCTAAAGTCTCAAAGACTAAATCCGTAATTGTCCTACGAAATCCGATATCACTACAAAAGATAAAGTCAATTTTTTCCTTTTTGAGTGCTTGTGCAAGTCTCAGCGAATCTAGTACGCCTTTATCTGAAAGCCTATCATTGCAGTGTCCCAAGGTAGAACAATGTGTATTAACGTGTTCTCCATTACAAGTGATGATGAGTTCCATGGGTATATAGGATAGCTTGGTAAAAAGAGGTTTAGGAAACCTATAGAAGTGATTGGAAATACTACCACGTAGATCAGTTTTATAGGTAGTTTTTTTTGTTTTCATTATGTTCTTCAAGTGTAGTGCCGTAGTGAATCTTTCCACTGGCATCATGGAGATAGTAAAGTGCTCCAGTTTTTTTGAAGTCTAAGGTTGCTGAGATTGTCTCTACGCTCGGTGAGGAAATTGGCGTAGGAGGAAGCCCGTAGTTTTGTCTTGTATTGTAGAGGTTGCTTGCATCTGAGAGATTTGCAAGAATTTGATTTCTACATTGGTCGTAGGTAATCTTTAGTCCATAACAAAGAGTTACATCTGCATCCAATCTCATCCCCTTCTCCAGTCTATTCAGAAAGATCCCGGCAATGATAGGCTTATTCCCTGCCAACTTCTCCTCATTTTCTATTATAGAAGCAAGAGTTAGAAGTTGATAACTATCAAATCCATAACCAAAGGATTGGAGCTGATCTCCAAGAGGAATCCAGACTTTTTGATTGAAGTTTTTAAGCTGAGATTTAATAAGTTGCTCTGGTATGGAACTATTAGGATCTAAGAAGTAGGTATCCGGATAGAGATAACCTTCAAGTGATTTTCCACTAGGAAGTGCTGAAAGAAAAGGAAAATCTGACTTCATCTCTTGAATAAACTGCTGATCTTCCGCCTTCTGAATGAACTGACCTGCAGAAATCTGTCACAGCTCTGTCAAATAAGCATCTATATCATAGATTGATCGTCATTCAAGGAGAGTCACACGTTTTACATCTCTCTGTGGTCACTCCTTAATGAGATTCATCAACTCAGCTTTCGTATATTTCCCACTCAGAACGTAGTTTCCCTCTTGAAGCATTGGGATTGATTCACGATGATTTCTCAATCGGAACTTCAGCATTATTCTTTTTAAGAATCCCTGTTCTGGAAAGAATTTATTGATAGTATCTCCTTTTTCTATCTGAACTGTTGTATTCTCTGGATAGTATACGGGAGCATTATAAGCACCACGTCCTAAGATCCGGAGTCGAGCAAAAAGAATGATAATTCCTCACATAAAAATTCGTCTGTTTTTCCTTTTTTTTGGGCGTCTCATGGTATAAGTATAAATCGATAAATAAAATATTCTGATTTAGAGTCGTTTCTTTTTCTTGAAGATAAGTACCGTGAAAACTGCCATTATCACCATAAACACCATAAGGACAATAAACGCCCCTGGATAGTTCTGGAATGGCAATCAGATATTCATCCCATAGATTCCACTAATCATCGTCATTATTCAGATAATTACCGTCAAAACCGTCAATATCCCAATATTTTTGTTGGTCATCATATTTGTAAGGGTATTGAATATCTCTCCGATGGAATCAATATTTTCATGGATGATTGAAATATGCCCTAGTATCTTACTTGTCTTGTATTCAAGATCCTCAAAGTAAACATCTAAATCTCCTTCATAGAAATTTGCGGTTACTTTTTGGAGCTCTACGAGAATTTCTTCTTGTGGAGAAATAATAGTTTTTAGCAATACCGTATTTCTCTTTTTGATAAGGACCTTCTTGAGAAGATCTGTATCAACAAAACTTTTATCAAAAACTTGTTTCTCCATTTCCATGAGATCAAGATTGAATTTATTGAGCCCAAGGAGTATCTTATCATACATCACATCAATAATCTTATAGAGAACATAGTAAGGAGAAATCTTGTACTCTTCTGCCTCTTCTCACTCTTCTTTGATTTCTTGTTCGTATTCCTGTCTAATCTTTTCGATATTATTGGTCGGATACTTGGTGATCGTAATAATAAAATCTCTCCCTAAAATGATATTCATTTCATTTGAAAGATATTTTCAGAGCTTTTCGTTGTATTTTGGGAAATGGATTACAAGAAAAATACAATCATCATACACATCAATCTTATCATGTGCACTTGGTTCAATCAAATCCTGCTCAACCATCTCATGAAGATCTAGGTTCTTGATTGCCTCTTCAAGTTCGTGATGGGTTGGCTTTGAAAGATGAATCCACTTTATTTTTCAGATTTTTAGAACATTTTTTAGCATTCTATTGATTTTGTAAGGCATTAAAACGCTCCACGAATTGGAGTATAAAGTTTTCTCTCTCTTTTGCAAAAGAAAAATCTGACTCTTTTCTACCTACGAAAGAAGCGGGATTATTTTATTTACATTTTATCTTGACTTTCCTAAGTAAAATGGTATCTTATCGGTATTGTCGATCCAAATATAGACAATATTTTTATCCTCTTTTGATTATAACATGAAAGGAAAACTCTTCTTTAGTCTCTTGGCCATTGCCGGGCTTATCGGTGTATCTTTTGCACAGGATAGCGGTGCCAAGGTTGGACTCTCAATGACTAATCTCCCTGCTTCATTTTCTGGAGAATTTACTCTTGAACTTACGACGACAGCAGGAGCTCTTTCTGGAGCAGAATCTTTTAGTCTCTCTTTTTCTGAGTGTGGAAGTGGACAGACTGAACTCTGGTATGGAAACCAGAAAAAATCTGATCAGATTTCATCTTTTTCTCTCGCTTCATGAGAGAAGAAATCTTTTCCACTAAAAATAAAGGCAAGAAATATCCATGGCTGTCAACTCAACTTTAAACTCACAGATACTGCAGGAGTTAAGGTTGCAGAAACAAGTATGGTGCTTAATTCAAACTGTGATCTTCCAATTGACTTTGATCAGGAGTATGTTTTGTCTCAACGAGCAAGCAATTCTCAGCGAGGTAAAGCTTGAGTAAACAAGACTACATCAGTAAATAATACGACAAGGGGAAGCTTGGATAAAGAACTTGAAAATGCTCTTGATTGGGCTGTTCGTCAAGGGATCATTTCTCAGGCAGATAAAGCTCGCTTCAATAATCCAATGACAAGAATGGAACTTGCACAGATGATTCTTCCTGTAGGTGACAAACTTGGACGCGCAGCTAACGAAGGGAAAGCATGTAGCTTTAAAGATCTTGTTGGAACCCCAGAAGCCAGCATTGCTACAGCAAAAAGAGTTTGCCAACTCAATGTAATGGGAATCTATCCAAATGAAGAAGTACTTGAGAACTTTATGCCAGATCAAATTGTAGACAGAGCTCAAATGTTGACGACGATCTCTAGGATGCTCTGGGGAAATAGCTATAACCAAGGAGGAAAGACCTTCTATGAAAAGCACTTTGACAAAGTAAAGGAAGAAAATGTTGTCAGAAAGCCATTTGCCAATCTTGTGGAACTTAGAAGTTATTTCTATCTTGTACTCCAGAGAGCAGAAGAAAAGGGACTTTTAAAATGGCAAAAGTAGCACAAGAATCCATAGATGAAGAGAAAAATCGAAATATTTCTCTGTCTGATATCCACTCTCGTGACTAAAAATCGGAAAGAGAGCGATAAAGAGATAGGAAGAATATAAAAAGTAAAGGCAGGATATTTGATTCTTGCCTTTTTTCTCTTAATTAATAAACTGGTTTCATTTATTTTTTGGATATGACAGATGAAAATTTACATCAAAACTCCACTGCTAAATAATTCACAAACAGTTCCCCATATGGTAGAGCACTGCATCAGAAAGAATAAAAAGCCTGATGTAAGGCGGTTCTTTGAGTCACATCTTCCTTGTGAGCATTGGATTAATTGAGAACGAACTCATATTTTTGTAGATTATTGAAAAGAGCTTGAGAGCGTCGTAAGAGAAATAAAAGGCTGATTAAATAGGGAAATTTATGAATTTGAAAAAAGACCATTCAAGGAAGAATGTAATAATAAGACTGAATGAAATATAATTTTTGAGGCAATGTTGCAGCAACTTATCAGTCCAGAGATTCGCCTAAACACCTGGAAGGGCAAAACCTGGAAAAAGGTCAATCAATACCACAAAACCTATTATCAAGATCAGAATTTTCTGATTTTTGAGGATCAAGTATCTGCTCATCAAGAGTATGAGTTGCAATTTATATGATCATGAGTTCGTCCCAGAATTAAAGTTGAAAATCACTTTTTTATTGAGGATTTTCCCTTTAATAAGAGTGTAATTTTTTGATATAGAAATTATGATGCCTGTCATTATCGAAAATTAATTTTTGCATGGATAATTTGTGAGACTTATTGTTGGTATTTTCAAAGGTATGAACTCTGAAAATATTTTTATGATATTACTGTTTTTGACAGCTTTAGAGAATATATTTGGATCACTACACCAAAAATAGATTATTCTTGTCTAGATAAAGATTTTTTTGAACAGGCAAAAAACTATATTTGTCAAATTCTTAATGACTTTTACCTTAAGGAAAAGCTATTCTTCGGGAATTATTTATATTGAGTTCCAGCTGAAAGAAAACAAATCATAGAACGATGTCGCGCATTCCCTTGGACTACTTTTCAGGAAGAATTTTTGCAAAGTCTTCTTTTGAGCACCAACGAAAGTAAGACAAAATAACCAGTAATGTAAAGCTATTTCTTCTAGTGCTCTTTTTTCTTTTGAAAAAGGAAACCAGAATACTACAATCAGTCAGATTTTTGGATCTTTTTTATTTTGAGAAACGCTATGAAGCCTGATCAGATCGCAACATTGCAAAAGGAAATTCTTTGACGGTATGCATTGCATGGGAGGAAGCTTCCGTGGAGGGCGACCCAAGATCCCTATGCTATTCATATTTCTGAGGTAATGAGTCAGCAGACACAAGTAGACCGTGTCATTCCTTATCGAAAGCAATGGATAGCTGAGATTCCTGATTATAAAAGTTTGGCTCAGCTCCCTAAACCTGAATTGCTAAGCCATCGATCAGGGCTTGGTTTTAATAGTAGAGCATTAAGGCTTCAACAGTGTGCACTGGAAGTCGTCGATCGTTATCAGGGGAACCTTCCGCAGAGCAAAGATCAGCTTTTATTACTTCCTTGAATTTGACCCTATACTGCAAGTGCTATTTGTGCTTTTGCATGGAATCTTCCAGAGCCTGTGATTGACACAAACATTAGGAGGGTGCTTATTTTTCTTTTGAAGCTGGAGGAAAGTATTTCTCTTCCTGCCTTGGAGCAACTTGCTAAAGAACTTATCCCTGAAGGAAGAAGCAGAGATCGGCACAATGCACTAATGGACTACGGAGCAACCCATCTCACTGCGAAGAAGACGAAAATTAAATCGCTAGGCAAACAAAGCAAATTTGAGGGGTCAGATAGAGAAGTAAGAGGACGAATCCTGAAACAACTCACTGGAAATACTTCCGTTCTAGGGAAGCAGTATAGTAGCTGGGATGAAAAAAAGCAAAGGAGAGTCCTTTTATTGTCCGATATTGTGCAGGAATTTCCTCATAAAGAGATACCGAAAATCCTCCAAACTATGGAAGCCGATCATCTCATTAGATATCAAGATCATGAAATCTGGATTGAATAAAGATTGGGAATTTCTGCGAGAGGTCTTTTAGAGTAGCTTGAAACTCGAAAGCTTTTGTCAAAGTGTCCCCAGAGATATTTTGAGGGGAATTAGCTCTTAGCATTTGGAATAGCTTGACGTTTGGAACGGAAAGATGCCTTAGGAAAACTAGCAAAGAGCAGATCTGAGCGTATGATGGAGCTATTTTTGTTTTTTGTCCTTCTTCATTGAGCCACGAGGGGAGAAAGACCAGCTTTTTTCTCGTAAAACAGGGGAAGTCTTTAAATCCTTACAGAACACGAGGGATTATAAATTGTGTTGGAGCTTTTGGCTTTGGTGGAGGGTATGAAAAGGTAACTCAAATACCTTGAAACAATAGAGGAAATGGAGTCTTGATCAGATTCAGACTTTACTTTTTCTGATAAAGGAGAGTGTCTCTGGAAGAGACTTCGTTGTCCAATCATATGCCGTAAGTGGCAAAATTTTGCTCAGGGAATGGAAAAAAGGAGCATCAAATTTAGCGTCTGAGGCGAAATATCGTCGCTTAAATGTCGAAGCAAGCTGATTTTTGAGGCGGTCGGGTGCCGTGCTCGTCAAGGGGGAGTAATTTAGGAGTCCGTAAGGAGCAGATTCTGGATAAGCAGGAGCATGCTCGAGAAAGTAGTCTAAAATTCCCTTACTAAAGTCTGCTCTTTTGTGGGAGTTTGGGTTTGCTTTTTTTGCCAAGTGATAGGAATGTTCATCTCTTGCTAGAACCCTTTTTGCATAGTGGAAGAGAAAGGAAGAAAGGAGTTTCGTTGTCCATTTCGTCTGGGGTCCAAAACCGCCCCATAGTTCAAAGCGTCTCTGCAGGACGGATCTCAAGTAGAGGAATGGGATATTTCGTCCATTGTGAGGGAAGGGTCTGGTTTCCTCGATCACTTCTCCACCTCAAAAGACTTTGAAAAACGAGCGGTAGGGGCTTTTGCCGAGGATCAATGCTAAATAGGTTAGCCATTTCGCAGGGAGAAGTGCTTTAATTCGCTGGGGGAGGATGGTTTGACTTCGTTGATGAGGCTGAAAAAACTGAATCTTTTCTCGGTTGACCTCGACTCCACGAAAGCGATAATACTCCGTGATGAAATTCAGATTTCTTTCTTTTCGTTGCTTGATCCATTCTAAATCTCCTACCTCTATTACGAGTTGCTCCACCTGATAATCAGAAAAGATCTTTTCAATTACCCCAAAAAGCAGCATTTCATCTCAGAAATTCTGATAACCATAATATCCTTTGAGATAAATCTGCATCGGTATCTCGTATTATAAAAGCGCTCTCTGCTCCCAAAGCACGAACACATTGGAAAAGAGCGAGGCAATCGTTAGCGGTCCAACCCCTCAAGGAACCGGAGAAATATGGAGAGCCCACTCCTGCACTGCATTGAAATTTACATCGCCAACCGCTTTCCCAGCTTTGTGCCCGTAGCCAATATCGATGATTGTTTGGGTTTTGTTCGGGGACAGGTAGGTTTCATCAATAAGATCAATCTGTCCCGTTCATGAAAAGATGTATTCCGCAGATTGACAATATGCTTTGATCTCGGCTGGGTCATCATAGATATCAAAAGATCAAACCTGAGCTCCTCTCTTGAGCAACTCAAGTGCTAGCGGTTTCCCAACGATGATACTCTTTCAAAGCACGGCTACTCTCTTTCCGCGGAGGTTTCCAAGCTTGTAATAATCAAGAAGGCTGATTACTGCCTTGGGAGTCGCTGGCAAAAAATCGATCATCTCAAGGAAATTTTTACCGATGAGCGTGCCATTGAGACCGTCGAGGTCTTTTGCTGGGGTGATCGCCGAAAGCAATTCGTTTTTGTAGGGCTGAAATTCTGGAGGCAAAGGAAGCTGAATAATAATACCCACACATCTTTCTTCACGATTAAGCCGATGAATGAGGGCTAAGATCAGTTTTTTGTCTCGTTGTGTTTGCTGAAGAAGAGCTGTCAACTCTGGATCTGAAGAAGTCCCCTTCCCTTGCCCAAACACCAATGCCGGCAATCCAATCTTTTCTCCGTAAAGCTGTTTGTATTTGACATACACCTGGGAGGAGGCATCATTTCCAAAAAAGAGAATAGCAACGAACCTCTCCTGATCTCAAAAAGTGGAATGCACCTGTTGTCTCAGGTCAGCTTCCATCTTTTCTGCAAGAGGTTTTCCATAGAGCAGCATAGTTCTTGATCGTAAGGAATAAATTTTCACTCCTACCTTACGAATATTCTTATCAAAAGCAAGCGTAGTCCCGTTTTTATGGAGCGATGGGGGAAAAAAGAGAAAAGAATTGATTAAAAAAAACTTCTCTTGATATAACAACATAAATCCTTATAGGTAATCATGTTAATAAAAAAAATAAAAGAATGAGGCTTACTTCATAATTGTGAATCAAACCTCCGAAGTCAGAGGACGGCTTTGTTACTCATAATCCTCAAAAGTAGGTGAGTGGACTACCTTATCAGAGAGAGAATTGGATACTTCCACTCTGGTATCCAAAAATAAAAAGAATAAAAAAAAAGAAAGAAAATGAGAAATAATATTCTATCATTAGTAGTATTAGTCTTGTTAATAGGACTGATATCGTGTGAAAAAAATGATGATATAGAACTTGATGGCAAGACAACATCTCCTAGCCAGGAGGTAGTTGTTGGGTCTAGTCTTGAATTAAGATCCTTACAATGGCATGACTTGCCATATGTAGAATTTATTGGTAGATTGTACAGAACGAGAGGATGTCAAAAACTTTCTGACAACCGTTTCAAGTTTGTTGTTGAGCTTCTTCCGTATGGAACTAGTAATTTCAGTCAGCAAGAACTTTATGTAAAATTTTATCACAATTCTACAGGGACGCAGTTCTACCAAAAGATGAGAAACGTTTCTCCTCCGCATCAACTTCCCAAGTTTGAATTGGAGCAGTCATTTCTTAAGACTGGAGACTATTCTTACCGATATTTGGTACGAGAGAATGCGTTTACTAATGATCTAAGACAGTTGACCGCTAAAGGATCTACATTTTTTTCTTTGACAATACCTTCTGGACTTGATGATTATCACGCCAAAGCACGAAAAATATATGCAGGAGCTGCGTATGGTTATATGGATCAATGGAATTTTGGTTATGAAGAATGTGTTAGTTGGGTAGCATTGAAAGTCAATCAAATGTGGGGAACTAACACGGAATTCAATAACAGAATGTTTGGTGCTCCGATAGGTAACGCCAAATATTGGAAGAAAAGATTTCAGGAGAAGGGCTATTCTGTAAATAGATATCCGCGTGCGGGTGACATTATTTGGTGGAGCCGTGGTGGATATGGTGCAGGGCATGTAGGATTTGTTCACGAAGTTACAAACGGGACAATTCGCTATACTGAATACAATGGAAGTATTCCTCATGGATATTCGTATAGAGAGTTATCGACCTCACAGCTTGGTGATGCAGAATTTATTCACATTCAACGCAAGCTATAAGAAAGGTTACTATCAAAAAATCCTCTCTGTTTGTAGAGAGGATTTTTTTATAAAGGGAGTTCGCAGGTTTTTATCTTGAATTTCCCCACTACTTTTCATGGAGGACCAATGGGATCTAAGAGTCAAGGATCACTCCTATACGGCTCATAGAGTTCTCCTTCGGTGTATTTATTGACAATAAGTTTATTTCATTTAAATTTTACGACATTTCGTATTCCCTCCTCAACCAATCACGAAGGATACTCCTCTTCTGCCAATCGCTTTATATCACCTCATGAAAACAACTTATCATCGATGAGGATATATTCCTGATCGTTATCAGACCCTCTAGAGCCGTAGAGAAAATAGTGATCTCCAGAAGCTCATCGAGAAGTTCGTCTAAGTTGCTCACATAGAGTGGGCTCTTCACATCTATAAAAGAATTGAGCCTCTTTATCTACATCTTTATCATCTACAACGAGAGAAAGGAGTCCCTTCTCCTTAATAGCATACTTCTCCAAAAAATCCATAAAACTTGAATACTCATCTCAAGGAGCATACACAAATCAACCTTCAGGAAGTGCACATACTTTTTGCTGTGTTTCATAATCTATATTATATTTAGGTCAATTGATATAATCCTTATAGAGTTCAAACGCCTTCCAAGGTCTAGATCCTCTACCAATTGTAAGATACCTGAGGTGTCCTTCTTCATAATGGCAATTAACTACTTGGTCTTCCAGCAAAAGAACTTCAGTATCCTTTAAGTCTAAAGGTTTACAATGGGGTAAGCTGTCTTCTACAACTCCCGTAGCCGAGGAATCCTTAGCGCTATTTGTCAGCATATGTCCAGAAGATGAAGAACACATCAATAATCACCCATCAGAAGAGGAAGAAATCGTTGAAATCTGCTTTACATCTTTTTTACAAGAGGTAAAGAACAATGAAAAGATTAAAAAAATCAGAAGTATATTACGTTTTGACATAGAACTCTTCATATTAAGGAATAAATCTTCTCATTTTTTATAGATAAAAGGCAATAATAATCAAGGATTTTTCTCTTTTTATCATTTAAGTAAACACTAGCACATCCTACTATGAAAAAGCATAAAATACCTTACCATCTCTAAAGATTCCCCCGTTTGCCTGTATTGCGGGAAGCTTTCTCGATCCTCAGCGACCTCCAATCTGCTCAGCGGGGTGGTTTCTGAGCCCTCAGCGACCTCCAATCTGCTCAGCGGGGTGGTTTCTGAACCCTCGGCGACCTCCAATCTGCTCAGCGGTGAGCTTTCTGAACCCTCGGCGACCTCCAATCTGCTCAGCGGTGAGCTTTCTGAACCCTCGGCGACCTCCAATCTACTCAGCGGGAAGGTTTCTGAACCCTCAGCGACCTCCAATCTGCTCAGCGGGGTGCTTTCTCGACCCTCGGAAACCTCCAATCTGCTCAGCGGGGTGGTTTCTCGACCCTCAGCGAGTCCCCTGCGACCCAAAAGGAATAGCAATCAATCGTATCTGTATAGTTTTTCTTCGTGCAATAAGGAGGAAAAATTTGCAGGATTTCATCTTTTTCGTATAATTAGGGTGCATTTTTATTTTTGATTACTTTTTTATGCAAGACCTCAGCACTATTCAAAAAATTGTAGATGATATTAAGTCAATCACTATCCAATGAGCAACCAACATTGCCAAGGCTGCCTTTGAGATTCTTATCTCAGAATCAAAAGCAAAAAGCTTTGAATCCTGATCAGCTTTTTTGGATTTTATTCAACAGGGGATGGAACTTCTTATCTCAGCAAGACCTACAGAACCTATGCTTTTTAATGGAATGGACTATATTAAGGCTGAGATTAGCAAATTAGCTCCTAGCGCCTCCGCTGATGAATGTCAGCAAAAGGTAGCAGAAGCAGCACAGTTTTATCTCAACCTCATCAATGAAACTGCCGAAAGAGCGGTTTTGAATGGGTTGGGAATTATTAATGATGGGGACAATGTCCTTACCCACTGTCACAGTTCCTCTGCCGTGAAAACATTGAAGCTCCATAAAGTAAAAGGTTTGAAGTTTAAAGTGTACAACACTGAGACAAGACCGCTTTTTCAATGAAGGAAAACAGCAAAGGATTTGATTGAAGCGGGGATCGATACCACGATGGTAGTAGATTGAGTAGCGCCGTTCCTTATGGATGAAGAAAGTGGAACAGATTTGATGATGGATTGTGTGATTATTGGTTGCGATGCGATCAAGCTTGATGGAGGAGTGATCAATAAGGTAGGGAGTTATAGCGTTGGGCTTTCTGCGCTGTTTGCCAATGTTCCGGTGTATATCGCCGGGAATCTCCTCAAGGTCGATATTCATGACAAAATTCATATCGAGAAGAGGCAGGATCATGAGGTTTGGGAGGATTCTCCAGAAGGATTACAGTTTTTAAATCTGGCTTTTGATAAAATTCCGCCAAAGTTCATCAGGGGGATTATTACAGAATTTGGGATCATCAAACCAAGGGATATTAGAGCAGTCGTGGAAAGAGAGTATCCATGGATGAAGATAGCATAGCATTTTTGATAGCAAAAAAGCTGACCTTTGGAAGGAGATCAGCTTTTTTTGGGGTTTTGTAGCGTTTAGCGAACTTTTTTGGTAAGATTGATAAGAAGGTAGGCCGTTGAAGCTGCCACAAAGATACTACTCAGGAAGGATACTACCACACCCACTCCTACGGTGAATGAGAATCTCTGGATCACGCCCGTTCCGAAGACAAACATTGCAATAAGCACTAACAATGTAGAAATAGATGTTCCTAAGGATCTCTTGAAGGTTTGTCGGATACTTTCATCAAAGACATTTGCGTAAGCGATCTTTTCATTTGAACGAATCATATTTTCCCTGATCCTATCGAAGATAATGATCACATCATTGATACCATATCCAATGATTGTGAGTATTGCGATGATGAACACCGTATCTATCTGTGTTGTTGGATCTAGTGCCATCCAGATTCCATACGCACCCGCTGGAATACTAATACTCAGCACGGAAGTAAACAAAACTACTGTTGCCAAGATTCCAGGAGCGATATTTTTTCTGATCGTTCAGAAGGAAAAAATCATATAGATCATCATAGCTAGCAATCATAAGCCAAGCGCTCGTGAAGCAGTATTTGTCATATACTTACCAACACTCGGTCCTATTACTACCTGTTCAACAATATCACTTTCAGAACTAATCGTCTGACTTTCGACAAGATAGTTGGAGATCGCTAAAGAGAGTTTATTTACCTCTTCGTTATCTTTGAGTCTATTATTGATTTTTATTTTGGTCTGTCCCACATTATTATCAAGGTGGACTTTAAGATTTGGGAAACCTTTTTCTGCGAGTCCCTGTGCCAACTGTTCTTTAAATTCAGCATCTTTCACCTTTCCATTGAAAACGACACTGATTCCTCCTGTAAATTCTTCTGAGAACTGTGCACTGAAGATGAACAACACCCATGACACTACCGTAGCAGTAATAGCAATCAAAATCCAGAGATAAGCTCTTTTTACAATACTAAAATACTTTCTCATCATCAAAAAAATAAAATAAAACCGTTCTAAAGAGTAGTTTTTTCCCTCCATTTTTCAAGCAGAAGTCAAGTTTTATTGTTTCGATGTTATTCCCTAGCTGCCGCAAAGTCGATTTGCTATTTTGGATAGCGTGATGAGCATTTCTTTGGGTTCTAGGCAAGAAAAAGGGGAGGATTCAGATTTCTCCCTATTTAAACCCCTATCCTCTGACGAGCTCAAGAAGAAAATAGCAATCTTTCATGAGCTAGACTAGTCGGCATATTTTCTCATGAGCGTTAGAATCTTGTTGATATATTCGTGTTTATATTTCTGCCTATAAACAGCGATATAAGAGGGGTGCTCTATAGGGAGAAGCTTCGTAGCTCAATAGCTATACAGCCCGTCATCAATTTTTACCACTTTTGGCTGCTTTAGAATGCAATCAGCAACTTCTTTACCACAAAGGAAAATCAGCTGAGGAGCATAGTTTTTGATCTCTTCTTTTAGTATTTCTCGGCCTTCCTCCTTTTCTCTGGAGCTAGGGTATCTCAATTTACCGTTTGCGTCTTTGGGGACCGCTCTCACCAAATTCAGCAGAGCGATTTCTTTGGTGGAAGACTGCAAGAGGGAATGGATGAGTTTCCCTGAAGAAGTCGTTTCGTCGAAAGCAGTCAGGATTTTTCCTTTTTTATCTTCCTTGTAAGAGAGTCCTAGGAAAGCGACTCCATGATACGGGGGAGTAGAAGCGATTTCCGAGGAGAAGGACTGATGAGAAGCTGTAAACTTGGGCATCGTTTGTGAGGAGCTTGTAAATTGGATGTGAAAAAAAACGACCACAATATCAATGCTGTGATCGTATGTTGGAATTATTCAATCGCCACCTTCGCACTCACGATTTCACTGATCTCCTGAGTAATTTTTGCCTGACGGGTCTTGTTGTAAAGGAGGTTAAGCTGATTGAGGACAGCAAGTGAATTGTCTTTTGCATTCTTCATCGCCAACATTCTCGCTGCGTGCTCTGAAGTTTTATTATTCAGCACTGCTGAGTAGATAATATTCCTAATTAATACTTGGAGGAAATCAAATTTAAAGGTTTCTATATCAGGTTCTACCATCAAATACTTTTTCTCGATACTTTCTAACAAATCGACCTCAAGCCCTGTATTTTGGAGGAAGGTCTCAAAGCTTTCCTCGTCTAAAGGATAGAGCTTAAATCTCGTAGACATCTGTATCATCGTATTTTTGAAGAAGTTGAAGTAGATCTTGATCTTCGCGTATTTCTTCTCTTCTAACGCTTGTCTAATAAAAACTGAGATCGGTTTTACTTCCCGGAGAAAGACTTTATCCGAAATCATCGCAGACCCTACAACGTTTCGTCCATTCCTCACGAAATATTCTAGCCCTCTCTTTCCTACAACGAAAATATCAACGTTATCTTTCGCATTTCCGTAGTTTTTCTCCATTCTCTTGAAGATTCTTGAGTTGATTGCTCAGCAGAGCCCTCTATCACTTGTCACCACAATCAAGAGTCTTCTTCCGTTTGGGTTCCAGGTCTTTTCATCGAAGTCAAAGATATTTACTTCCTTTCTCACCTCTTTTAGCACCTCCAAAAAGCTTTCCATAAAAGCTCTATATGAACCGATCACGCCTTTCAGCTTTTGAAGCTTTACGGTAGAAACAATCTCCAATGCTTTGATAATCTTTTGGAGATTTTTTACGGATTTAATTTTGGTTCTGATCTGCTTGGTATTTGCCATACAACACAATAAGCTAATAAAGCGTTACTTTCCTTCTGATTTTTTCTTTTTGATTCTCAAAAGCTGGATATCTGCGGTCAATTTTTGGAGGTTGAGATATGCTCTTTCAATCTCCTCTTGATCCCCTGTCGCTTTGATTTCGGCGATCTTTTTTTCAAGATCAATTTTCATTTCCTGGAGCACCTGATCATCAGACTTTGGATTGGTCGTTGCAGAAGCGACGAAAAGCACAACTACGTTCCCGTCTGTATACATAAATCCTTCTCCGACCGCCATTGCCACCTTTTCATCTTCGAAAAGGAAATCCGTATCAGAAAGAAACTCACTTCCTTTTTTTTCTATCGGTGTAAATCTCACAATTCACGGGATTGTGGTCGTCGTAATCGGATTATGATGCGGTAAAATACAGAGCACTCAGACTTTACTTGGCAATTCAACTTTTTCTACCTGTCCATCGAAAATTACTCCATGAACGGTATGCATCTTTAGCCTCATTCCCCTCTCTTTGATAAATAAAAGCAATCTGATCTTTGTCGCAAGGAAGACTTGCTTCACTTGGAGAAAATTATTCCTGACTCGTGAATCCTCCTTCCTTTAATATCTCTGAAATAAGCTTTTTAATCTCAGCCTCAATCTCTGAAGTAAGCTCCTTAATTTCCTCGATTTTCTTTGCCAACTCTAAATAACTCGTGTCTAATTTCTGGTAAACTTTTTTCTCAAATTCAGCAATTGCCTCAACCGGCACTTTTTCAAAGTAGCCATTAATTCCCGCATAAAGGAGCACCACTTGCTTGAAGAAAGGGATTGGGTGATCGTTGCTTTGCTTGAGCATTTCCATAAGCTTTTTTCCATTATCAAGCTTTTTTCTTGTGGCAGGATCCAAATCTGAAGCAAACTGAGAAAAGCTCGCTAATTCACGGAAAGTCGCAAGTTCAAGTCTGAGTTTTCCAGCTACTTTTTTCATAATTTTGGTCTGAGCAGAACCTCCTACTCTAGAAACTGAGAATCCCACATCAATCGCAGGCTTGAAACCAGAATTGAAAAGCTCTGTCTCCAAAAAGATCTGCCCATCAGTAATAGAAATCACATTGGTTGGAATATAGGCTGAAATATCTGAATCCAAAGTCTCAATAATCGGCAAAGCCGTCAAACTTCCTCCCCCTCGTGGCTGATCCACTCTCGCAGCTCTTTCAAGCAGTTTTGAATGAAGATAAAATACATCTCCAGGATAGGCTTCTCTTCCAGGAGGCCTTCTGAGGAGCAAGCTCACCTCACGATAGGCTACCGCATGCTTGGAAAGATCATCATAGATAATCAAAGCATCTTTCCCATGCTCCATAAAGTATTCTCCCATTGCACATCCTACATAAGGAGCAATATATTGAATCACAGAAGGAGAAGAAATCGGAGCATTGATCACAGTCGTATATTCCATCGCTCCTTTCTCTCTGAGCATTTCAACGGTTCTTCTGATTTTTGAATCTTTTTGTCCAATCGCGACATAGATACAGTAGACATTCTTTCCTTTTTGATTGAGGATCGTATCGGTCGCAATAGTCGTCTTTCCGGTTTGTCTATTTCCAATAATCAATTCTCTCTGCCCTCTTCCGATTGGCACCATCGCATCAATAGACTTAATTCCGGTTTCCAAAGGAACATTCACTGACTTTCTGGTAATTACTCCAGGGGCTACTTTTTCCACAAGCCCAAAATGCTTTGGAGTGATAGCCTTTCCTCCGTCGATCGGTTCCCCGATTCCGTTGAGCACTCTTCCCAAGTATTCTTCTCCTACTCCAACACTGAAGACCTGACCAGAAGAACTCACACTGTCTCCCTGCTTAAGAGAACTATAATCTCCGAGCACGAGGACACCAACTCCATCGGCAGAAAGATCCAAAACTAAACCTTTCAATCCATTTTCAAAAAGAACAATTTCTGAAAACATCACCGTATCTAACCCAATCACGGTAGCCACCCCATCTTTGAGTTCCAGAATTTCTCCCTTTTTAGTAAAATCCTCATTGAGCTGGGCATGGTTAATCCCTTTTTCAATCTGCTGTAATAAGTCTTGCACTACTGACATGATAAGTGATAATAAAAATAAATCTGATACTTGCTTTGATGAATATTTCCCTCTGTGAGGCTAAAGAAGATCAAAGAAAGCAACCTCCTTCTTCCTTCGTCCTCGCAAGAGAAACGAGGTAAAAGCTTTTTTATTCAAGTAATACTTCTAAGTCTTTTTCAAGTGACCTCTTGAAGATATAGCCCTGCCCTTTCATCTTTACAGTTAAATTCTCTGAGGCAAGTGGATGAAACGCCACCTCAACCTTTCCAAACTTTTCTTCAAGATAGGCTGTCAGAGGAACAATAATATCCTCATCATTCGATTCCAAACGAAACTGGAGATGCTCTCTCGGTAGCTTTTCCTGAAACTCACGAATAAATGCGGATAACTTTGCTAAGTGAAGTAACTTTGCTTGTTCGAGGAGATGAATCATACTCGCTGTATCAGCATCCCCATTTTTTATCCCCTCTAAGAAGCGAGGGAGTGTCAATTTCTTACCATATTTGAGAAGTAAGGCTCTAATGACGGCCAACTTTTCCAGAAGTTTCTGAGGATGTTTCATCTGGAGCAGCTGTTCAAGGAGTTGTTTCTTGTTTAGCATCAATAGGATTGAAATATAAATCTTAGTAATATTAGGTTGTTTTTAGACGGATGGAGATTGCAAGGACGCCAAATGTTTTTTCTTCTTCTGGAGTATAAAACTGGTAATATACCTCTCTCCCATGATCAATCTCAGTAATTCCCGGAAGCGTATGCTTGAGTCCTTCATTTTCTAGCATTGCCTTGAAACTAGGGTAGTGTGTCAAAGCAATAATCTCTAAACATTCTCCTGTATCTTGAAACTGGAGAAAGTCGCCGACTTGAAGTTCGGAAAACTTTCCTTTGCAAAGTCTCCCTTCTACCGTTTTCTTTCCTTGAAGGATCAAGGATTTGTAAGGCTCCCCGACCGTAAAATAGATCGTCTTCATTATTTTCACCGATTCGTGTCTAAAATCAGGTTACTTTCTTTTTTTTGAGGGGAAGCCTCCTTCTCACAGGCGAGAAAAGGCGCCTTGTCCTCACACGATGGGAGTTATACCCATTCTCCTCAAGAAATCAAGCCTTCCTCTCCAGAAACTTCCCAAACGCTTCTCTTCTGGCGATACTAACAAAGAAAGAAATCTGAGATTGGAGAAACAAAAAAACCTCGCTGCTAAAGACATCACTCCAAGACAATAATTTTTTATTGTTCTGCTACTTTAGTGATTTTTTCAGCAAGTATTTCTTGTGCTTTTTGAAGCTGATTGTCGATTCCTTTTTCTACATAACCAGTAAAGTCAAAAGGAAGTTCCACGTCTGGCTGTATTCCTTTTTTGTCAATCGTAGTACCTTTTGGTCAGAACCGCTTCCCGATTGTATATTTTAGTGATGTTCCATCGTCAAAATCATAGAGGGTCTGAATTGAACCTTTTCAGAAGCTTTCAGTCCCGACAAGTTGAGCATTAAGCTGCTCTTTGAGTGCAAGAGATAGAATTTCACTCGAACTTGCACTTAATTTATCAATCAATACTACTAGCGGGAATTTTTCAAGCTCTCAAAAACCTTTTGATTGATAATCTATTGGCTTAAAATTGGAATATTTTGTCTTCGTAATAAGAGTCCCTTTTGGTAAGAAGTGTCCAGCAAGCTCCACCGCACTGGTTAGTAGTCCGCCACCATTTCCTCTTAGATCAAGGATGATCCCTTTTACTTTAGCTTCAAGAAGCTCTGAAATTGCTCTAGTAAAGAGCCTATTGGTTTGATCTCCAAAAATAGACACTTCGAGATATCCAAGCAAAACTCAGCTTTGATTCAGAATTTTTGATCTCACAGAAGGAACATCAATGATACCTCTTTCTACAACCTTTTCGAGATAAGATTTCTCACCGGTTTTCTCAACTCTCTCAAGAAAAAGCTGAACCTTCGTTCCTTTTGGTCCCCTGATTTTTTGAACCGCCTCCATAGTGCTAAGATCCTTTGTCTCTCCCGTCCCGATCATAATAATTCTGTCTAATGGCAGAACACCCGCTTTGAAAGCAGGAGAATCTTTGACCACCTCTTCTACCTGAATATAATAATCTTTTTTTCCGACTACGGCTCAGATTCCCTCTATTTGTCCTTCTCCTTCAAGTTCTGTTTGGAGTCCTGAAAATTGCTCTGCGTCAAGATAGGAAGTATAAGGATCTCCCAACCCATCAACGAATGCCTTGGTCGCATTTTCGATCATATTTTTCTGACCACTGAGGAATTCTTCCTTGTCATAATATTCTCCCGATAAAAGCTGAGAAATTTGTTCCATACGAAGATATTTATAACCAATTTCTGAGAATAATGTATTTGTCGCTGCCGAAATTCCATATCCCCTCCAAAAAATGAGAGCCCCTCAAACAAACATCCCCAAAGTAAAGATAAGCATCATCTTTTCTCGTTCTCTGCTTTTTTTCCACATTGTGCTTGTAGTTATGATAAAAATCACTAGAACGAAGTGTAGCTATTTACTTAGAATTTGCAATGAAGAAACAAAAAAAAGCTGATCTCCCAATCTCATGAAGACCAAACCTCTATGATTACCTAAAGATTCTCGCTCTTATTACCATGCTTATTGACCATATTGGGTATTATCTTTTCCCAGAAGTATTGGTCTTGAGATTGATTGGAAGAATTGCGTTTCCGACCTTCCTCTTTTTGGTTGGATTTAATGGTTCGTTTCGCTGGAGGCGGGAGCTTTTTTGGCGAGGAATAGGGATTCGAGCATTCACTAGCTTTGTCGCTTGGTATTTTAGCTTTTGATCCTTTGGAGCAAACATCCTGATTGCAATCCTCCTTGCAAGAGGACTTATACATTTTTTACTAGAAACGAAAAACAAAATTCTTTGGTATGTTTCTTTTCTCTTACTACTCCTTAGCCATTCTCTGATGCTGAATTTTCTCGACTACGGAGCCTTATCTTTTTTCTTTGTCCTTCGATGATGGAAAGCATCTAAAGATAAACAGTGATTTTTTCTATGAATTCCTGTAATGATTTGGCTCTCCCTTCAAAATATTCAGGTTTTTGGATTCTGAAGAGTAAACGGAAACTGGACTCAATACTGCATCCTCATAGCATGCTTTCTTATACTTATCTTACTCTTTCGGATCTTGACTAAGAAAAATTATACACTCTCTTGGAAATGATGGTGGAATAAAATCATTCTACGGATTTCCCAGCATGCACTTTTATTTTATGGAGTGCATATTGTGTTGTTGGTTATCCTAGGATTATCGAAGTTTGGGCGTTTGTAGTTTTGGGTCGTTTTTTATTTTTTTACTCTCTGTACTTCATAATAATCTCATTCAGTATGATAAATAAGCTGATACCGCCGATTTTTATCTGAAAGTCTATTGTAAGAGGTTGGGTGCTCTTTCCCTTCCTCTTGAAATTCCTTTTCTTCTCGCTTCATTGTCGCTCAATCTATAGGGAAGCTTCGTGTGCCATAAGCATATCGTGAAATATAGATAGCATTATCGTGATAGTAAACTGATGCAACTCCTCCAATTCCGGACCATAGGGCTTCCCAATTTTTATCCACTTCTCAGCTAAAGGGAATCAATTGTAAGCCTTTATCGGAGCTTACTATTCCCAATAATTCATCTAAACTTAACACTTTAAAATCATCTTCTTTCTTTTCTCTAAATAGAGTATAACCTGCTTCTGACTTTGTGGCCCAACTATTGCTAGTCCATCTATATATCCAATTTTTGTCAGAACCGATACATCTTGCATATTGCCTTCATCAATTTCTACAAAATGTTTTACCTTCTCCTTTAAGATAATCTTTCCGCTGCTGATCATTTTTAGATCAATTATTATCCCAAAATTGGTAAGGATATGGGCTAAAAGTTTTTTCATCTACTGGAAATTTATAAGGTGGCAAGACTAAAAACCCCTCACTGTATCCTATTGCTCTATCAATGGATTGACTCCCTAAAAAACCAATAAGCTTAAAGGATGGCAATTTGTTTTTTTTCACCCCACTCCATTTTATGTTCGGGTCCTTAATTTTTGGATTTCCAGGAGAATAATAACTAGGAGTTGCACCAAAATAGTAATAATTTTCGTCTTCTAAGTAGCTATCAAAGATACCTCCGTTAATATTTTTAATCTGATTTTCTGTGGGTTTTACGTATTCTGAGCTAAATGTCGTATATTTTCGATTTTTATCTGCATAGAAACTTCAGATATCGTAATCATTATACTCATATCTTCCATCGATAATATCTCCAATATTTAGTAAACTATAGTTACTAACATCTAGAGGACGATATTCCTTAGAAAGGCTAAAATATAGACTATCTTCTGATGAGGAGAAAGATTGTTTAAGACTGTTTCAAGTTTCTGGAGCTATACTTCAAGTTTCTGGAGCTATACTTCAAGTTTCTGGAGCTATACTTCAAGTTTCTGGAGCTATACTTCAAGTTTCTGGAGCTATACTTCAAGTTTCTGGAGCTATACTTCAAGTTTCTGGAGCTATACTTCAAGTTTCTGGAGCTATACTTCAAGTTTCTGGAGCTATACTTCAAGTTTCTGGAGCTATACTTCAAGTTTCTGGAGCCATACTTCAAGTTTCTAACCCAATCTTTGGTTGGACATCAATATTCTCAACTTCTTGTAGAAAGCGGCTATTACTTTTTGTATAAGAAAACTTGTAAATAAAAATACAACAAATTCCAAGTCAAAGGAAGATAACTATCAAGAAAATTTTCTTTTTCATTTTTCTTCTGAAGAAAATTTAAAAAAATAAGTTCCCCAAAGATATACTCTAGGGAACTTAAAAATCAAGTTAAATCTATTCTATTGCCGTTTTGTCTGTACATGAATAAAGGAAGATGCGAACTTCCTACTATTTTGCGCAGCCCCATACCTTTGCAATACTCTTTCTGGGGATTCGTATGCATTCAGACTGAGAAAGTTATACTCAGTAATTACAACAGTATTACCACCATCTCTTACCTCATTTACGAAAGCTACGTGACCATTGGCTCCTGAATTCCCATCAACATTTGACTCCCACCACGCAATATCATCGACCTTTGGATTCAAATCCGCAGAATAACCTTTAAGAAGTAGCTTAGACTCCCAATCTTTTGCATTTCCTAAGGAATTACTATTAGCTTTACCATAGAAGCCATTTTCAATTCCCCACATTTGGTTAATTTTTCATGCGCTTTTCTCTAAATATCAACCTAAAGTTGTAAAATATATTCCTACAATAAATAATTAAAAAGACTGAAAAATCTGAGTCATTAGGGAAGATTGATCTTTATCCTCAGATTTTTTTTTATCTTCTACAGAAAAATGAACATCTTCTTGAACTTTATTTTTGTCAAGCATAGCCACCTCATAGGATACCCCTAAAAATCAATGATCCTTATTTGATTCTAGAGAAAGATCTGGAAACGTTTTTAAGGCTTCTTGATAATCGTAATCAACCTGTTTGAGTATCCAAAATTTTTCCGGAGTTCTGAGCACTCTAGATATTAAAATATCCCAAGAAATAGTAATATTTCTTTTCCCCTTTATGAGTTCATTAATTTCGCTTACCCTTTTCCCAATATAAAGAGCAAAAGTTTTTTGTTTTCGTCATTGCTCTTTTAGGAATTTTTCGAGCAACTTTCATGGATGCTCAATCATATGATATGTATTTCAGAAATAAATTTTTCACACATTTAGTGAATTTTATTTTTTTTCACTATTTCTATGAATTTTTCCCAATAAAAAACCACAAACCAATTATAAACCCTTATTATTTTTTTGCAAATATTTTCATACTTTCTATGAAACAGTGAATTTTATTGTTCAAACTTTTAAATATTCAAAAAATTCACTAATTAATAGAAATAGTGAATTTTTAGAAGAAAGATATATCTACCCCTTTACAGAAAGTCCGACACTAAAAAGCTGAAGATTTCACCTTTTTGAATCTTTTTTCTTTCCATATAACTCATCTCCCACAATTGGATAGCCTATCACGGAAAGATGGCTTCTTATTTGATGACGGATTCATTTCTTGATTTTTACGAGTAGAGTTGTAGTTTTACTCAACTCATTCCATTGAAACTCTACAATTTCTGTTTGCACACGATGACACCTCATATTAGCTTTATTTTTTTTATCTTCATTATCAATTACTACCATTTTTTCAGGAGCAAATTTGTGATGAGCAATTGGAAAATCAATCACTAACCCATTCTTTTCTACCCGATACTTAATATCTCCATAGACCTCAAGCAAATAATACTTATGAACCATTCCCGCTTGTTGAAGAGTTTTATAATTCACTTTAATTTCACGAGTTTTCGCAAAATAGAGAAGTCATGATGTCGCATTATCCAATCTATTAAGAAGCCCTCGTTCTTCATCTTCGGTGAAAGCCTTGGAAAGTAGGCTAATAAATGGGTAACTTCAGCTTTTTCCCGATCATTCAAAGAGATATTCAAGAAAGGATTTTTCTTTTCCAAAAGTTGTTGGAATACCTCAGGGCTTTCGGACATAGTAGCAAAATTCATCTTCGTATCGAATTACTTTCATAGCTCTCTTAAAATAAAACTCTTGGATATCTTAATTACAACAAATTAAAACAAGAAAAAAAACCTGAATAAAATTCAGATTTTCCTCTTTTTATTCTTCTTTTTTTTGATCTTTATCAAACGCTTCAATTACCTCCTGCAAAGTTCCTTTATACATAAAGTATTCTTCAGGGATTTCATCCACATCACCATTAATGATTTTCTCAAATCCCGCAATGGTATCCTCAAGTTTTACATAAGTTCCTGGCATTCCGGTAAACTGCTCAGCCACCGAGAAGGACTGGGAAAGGAACTTTTCAATTTTTCTGGCTCTATAAACCGTTACCTTGTCTTCCACTCCAAGTTCTTCCATACCGAGAATAGCGATAATGTCTTGAAGTTCCTTATATTTTTGGAGGATTCTTTGGATTTCTTTGGCTACTTTATAGTGCCTTTCTCCTACTGCATCAGGATTCAAAATCAGAGAATAACTCTCAAGCGGATCCACTGCCGGGTAAATCCCCTTTTCAGCGATAGACCTATTCAACACGATCGTAGAATCAAGATGCGTAAAAGTCGTTGCAGGAGCAGGATCCGTAAGATCATCCGCAGGAACATACACCGCCTGCACCGAAGTAATAGACCCTTTATCCGTAGAAGTAATTCTCTCTTGCAAAGTTCCCATCTCACTCGCTAAGGTTGGCTGGTATCCTACAGCAGAAGGGATTCTTCCAAGCAAAGCTGAAACCTCAGATCCTGCCTGAGTAAATCTGAAGATATTATCAATAAAGAGCAACACATCTTTTCCCTCTTTATCTCTAAAGTATTCTGCCATTGTCAATCCTGTCAAAGCTACTCTCGCTCTTGGACCAGGAGTTTCGTTCATCTGTCCATAAACCATCGCTGTTTTATCGAGTACCCCAGATTCCTTCATCTCGTGATAAAGATCGTTTCCTTCTCTGGTTCTTTCTCCAACTCCAGCTACTACAGATACTCAGCTATGCTCCGTTCCAATATTGTGAATCAACTCCATCATTACGACCGTTTTTCCTACACCAGCCCCACCAAAAAGTCAGACTTTCCCTCCTTTCAAAATTGGAGCAAGCAGATCAACGACCTTAATCCCCGTTTCTAGAATTTCAGCTTTTGTCGCCAAATCAGCGAAAACAGGAGGCTTTCTGTGAATTGGCCATTGCTCAGTTCAAGCAATCTGGTCGCTTCCATCAATCGTCTTTCCAAGTACATTGAAAATATGCCCCAAAACCTGAGGTCCAACAGGGACTTGAATCGGATTTTTGGTATTTTCGACTTCATCACCTCTTTTTAGTCCATCAGTAGACTCCATTGCGATTCCCCTCACGATTCCATTCTCTAGAATCTGTAAAACTTCAATTACAACCTCATTTCCATTCGCATTCTTTGGTTTTACCTTGAGAGCATCATACACCTTTGGTGATTCCTCTTCAAAAAGCACCTCAACCACCACACCTTTTACTGAAACAATTTTTCCGTTCATTTTATATCTAAACTAAAGTAAAATATTTTATCCTTAAGGATTACCAATCTTCTAGATGGATTTCTTGTTTTAAAACATTTCCATAAAAATCCTCAACCTCTGCTCTCAAATTTGCAGAAGACGTTACAAAGCTTGTGGCTTGGAAAGAAAAGGTATTAACTGGTTTACCTCAGCTAATCTCTACAATTTTTCCTCACTTTACTCCTGATATTTCATCATCAAAAAGCAAACTAACATCGTATTTTCAGTCTTCAGTTTTTTCTACCCTGCTTTGCTCTTTTCTAAAAACTGGCGGGGTCTTATCTTCTGAAACGAGATTAATTTGCGTACTTACTGTTTTTGTAGCTCCAGCCTCATCAATTGCTGTAATTGCCAAGCTATGATTACCGTTTGGATAATTAGAAAGATCTATTTCTTTCGAAGCAAAAATTTCAGTACTATTATCTATAAAAGATTTCACCGTAGTTCAATCTAAAGTTATATTCACTTTTTTAATTGGTAGTGGCGATCTAATACTGTAAATCACTGCATTTTTCTTTGCGAAGCTTCACTGTCATTGAGGCTTAATAATACTGATTTGAATATTTTCATTAACACTTGGTTGCCTATTTTCACAGAAATTCTCTGGCTCTTCTGCGAAAAGATTCAATCCTCATACAGCTCCTGTTCCTGCTTTTCCTTTTAAATAATTTTTGATATCTTCTAAGTCCATTTTATTGGGCATAAACGTTGAAAGCTGAATCAAATATCCTTTTTTCAACTGATCAGCAGGTGTGAGAGGACTCGCTTTACCATAGCATGAAGTATCTATCTCTATTTCTGTAATTGGTCATTCCGCTTCTTTTGGGAGATTTCCATTCCATCCGAGCGTCTGTCCAACAAGGCTAGGAGGAGTAGTGTCTCCAGGAATTTTTCCAGAAATAAGAGAAATACTCAAGCTTGTTGTATCTTTATTTGCCATTTCCTCGTTGGTAAGATAGTTATGTTCTAATAAGCTTGAGAGAAATGACTTTATACTGTTTGCATGGATCGTTCCTCCATAAGCATTTCTGTTCATAGGAGTAGCATTTGCATTTCCAGCCCACATTAAAATGACTTTACTTGGGGTATAAGCTGCAAGCCATCAATCTCTTGGACGTCCTCATTTATCTGTTTTTACGTCAGAAGTTCAGGTTTTTAGAGCATATGTCAATCCTTTAACATTAAACTTTGTCACCCAACCTCCTAGTCTGTTATTTGGATCAGAAAGAATTTTCCACATCAGCGTTACAATTCATGGTTTTATGACATTTTGTTTTGTCTTTACCTCTTTTTTGTAGAGGATCTTGCCATCACTTGCCTTAATCTCAAGAATTGGATTTATTTCGGCAGGCGTCGGAGTAGAAAGGTGTTCGTAGGCACCTGCAAGCTCCAACATACTTACTTCTCCTGCTCATAAAGCAAGAGGATATCCGTAAGAGATATTGTTCTTGATACCAGAAAGTCCTAAACTCTGGAGAAATGGTTTTACTACATCCTCTCCTCCAACTGCAAAGAACATCTTTACTGCGGGGATATTTCTACTGAATCCAAGAGCCTTTTTGAGAGGAAGCATTCCATCAAACTTTCCATCAGCATTATTAGGAGTATCCTTTCCTGCCCTGAATAAAATATCATAAATTGGAGTATCTATCGTAAGAGGAAGCTTATCAAAACCTAGTGCATACACCAGAGGTTTTATTGAAGATCCAGACTGCCTAGGATTTCTTACCATATCGTTCTGTCCCTGGATTTCTTTATTAAAATAATCAAGAGATCCAACATACGCAAGCACATCCCCATTATGAGAATCTAAGTAAAGCATAGATCCATTTGTTGCACCATTCTCAAAGAAAGATGACTTATTATTCGTAAGTGCTGTTTCTGCTAGCTTCTGAACTTCATAATCCAATGTCGTTTTGACTATTAGTCAACCTTCCTTTGTGATTCAGGTTCCATATTCTTTTTCAATAAGATCCTTAATCCAGAAAATAAAATGAGGTGCCTTAATATCAAACTCACTTTTTTCAAAATTTTTGGTAAAAGACTCTATAATAGCTGCTTTTACTTCCTCTTCTGTAATCTTTCCATCATCGAACATTCTTCAAATTACAAATTCGGCTCTTCCATTTTCATAACTGATACGATAAGTTTTTCCATCCAAGCTTATCTCTGAAGGACAAAGTCCTATAATAAACTTCACAAGAACTTCCGCATTTTTCTTTGATGCAAGATTAGCCTGTAGTAAATTTTCTCTATATTTTGTCAAGACGGCAGATTTAATATTTCCTTCAAATGGATACTCTTGACCACTCGTATCAACAACCTTGAAGGATCCTAGCAATAGGTGAGATTTATAGGGATCATATCTTGTAGGAGCCTTGGGAATCGAAGCAAGAATTGCTGATTCTAGAACTGTCAAATTTTTTGCTGATTTGCTGAAATAGGTCTTACTTGCAGCTTCAACTCCATAAGCATTATTTCCTAAGAAAACATAATTGAGATAAAGCTCAAGAACCTTATTTTTCATTTCATGTTTAACTTGCTCACTATTCAGACTTTTATTTTCTAGTTTTATCTGTTTTTGTAGAACATTATTAAGCCTTTTAGCGAGGACAATCTGCTTTAATTTATAATCTACCTTATCAAGAAAACTACCTCCAAACGGTCTTTCCAGCTTCATTACATTGGTTATCAACTGCTGAGTAATCGTTGATCCTCACCCTCCATTCTGTCCTAGCATAGTCCTAAATCCGGCTCTGAAAATCCCCATTGGATCTAATCCTTCATGCTCCCAAAATCTTTGGTCCTCTACTGAAACAATAGCATCAATCATATGTTGACTAATGTTTTTTAAATCAACATATTCTCTATTTTCGTCAAAAATCTTGTAGAGCTCGTGACCATTTCTGTCGGTGATAATTGTCGACTGCACCATTGTCATATTTTTTATTTGGCTTACATCAGGAAGGTTATTTAGTATTCTTAACTGTAAATACGCAAATCCTGAAAAAATTCCAAGAACTACCAGCCCTGCCACCATTCGTAAAATCTTCTTTACTCGCCTTTTCTCCTTCCAAGAAGTAATTTCCTTTCAGGAATTGAAAGGCTTTTTAATGAAACTTTTTGGAGTGTAGGCATACATTGGATTTCTCTGCATTAAATTAGCGATTAAATAAAACTAGAATAAAATGCTGTTTACTTATACGTATTCTATCTCTGAAAACAAGGATAAAATCTGATTTTTTTTGTAAGAAAAAACACCTCGCATCAAGGTGTTTCTTCATATAATAAGTGACTTGTAACTTATGGATAAATGTACCCCACAATACCATCACGATTCGCATTTTCCCATCTCTGGGTAACAATATATTTTCCTGCATAGTTCATATCTTCAATAATCATTTCTCAAGAATCTGGATGATATTCCTTTACAATTCCAACATGTCCTGCATAACTTCTAAGCTGCCTATAAACAATAATTGCTCACGCTCTCGGAGTCGATCCAACATTATATCATGCGGCTTTTGCGTTAGCATACCAATCCCTCGCATTTCCTCCAAATGGTCTCTCCTGCTTGGTATCACTCGTATAGCTAAAGATTCCCGGAGCCTTCATCGCTGCATACCAAGTACAATATCCTACAGCAAATCCGTTTGAAATCTTAGGATTATAATACCATTGTCTGATAATAGCAGATTTCGCATTACCTGGATTAACCGCTGAAGCGCTTCCACCATTGGTATACACAACTGCTCCCTTTGTGTAAGTTGCTGTTTTCTTCGCTGTTTTTGGTTTAGCAACTTCATCTTTTGGTAATATTGGCTGAGCTTTTTCTAGTAGACCTATATCATAAGCTCTCTGTTCTGTCACATTAATAAACAACTCCTGACCAGGATACAAGATTTCACTGTCATCAGAAAAGTAATTGAAGGTCATCAAGTCGTCGATGTTGAGTCTATAAAACTCGGCAAAAAGTTTCAGACTTCGCTCTTTTTCTACGACATAAAGAATACCTTCTTCATTGTCACTTACAACCAATTTTTGACCAACCTTTGGCTGATCCTTTATCTTATTTAATCTCTGAAGATCCTTTATAGGAACTCAAAATTCTTTTGATATCTTTTCTAAACTATCACCTTCCTGCACGATATAAGAAACCACAGGAGATTCCTCACCATTATCAGAAAGAGTTATATTGTCTGGATTTATTCCAACATCCGATTCCTCCATTCAAAGGAAATCAGCATTAACAGGGACAGTAGCATCTTCTCCAGAAGATCAGAATGCATCAACATTCACTAGCACCACTGTAAAGAAGACAATCGAAAGGATTGTAAATAAAAGCTTTGTTTTACTTACCTTGGTTTTCCTTGTCATACAATAAATACAAGATAAATGGCGGAGGGAGTGGGATTTGAACCCACGAACCGAATAATCGGTTAACAGTTTTCAAGACTGCCGCGTTCAACCACTCTGCCATCCCTCCACAAACACATCAATTTACCGCACATAATCTACTGGTTGAACAATTTGCAGTAGACAGTATATGTAAATGCTCTTGATTTTCAAGACAAAAATGCTACATTAGATTCACTAGTTCTTTAGTGAACTTATTCAGGTTTTTAATCAATAATTTATTTTTAATTTTAAAATAATGTCTACTTCCGACAAACTAAAGATATTTTTTCCTCTTCAGCTCTGAGAAAACAACTCTATTCTTAGAAAAAAATCTCAGAAAATAGAGAAAATTACTCCGGAAATCAGGGAATTCTGAGAAATACTCCTTGAACTTATGCGAGAATATGATGGAGTCGGGCTTGCCGCTCCCCAAATTTGAGAAAACATTGCCATGATTGCTACTACCCAGTGGAAAAAGGTCCCACAAGGAAAAAATGCAGACAAAGATTATTTGGGAGAAACCTTACTCATTAATCCTGAAATTGTCGACAAATCGAAGGAAATGCAAATTTCTGAAGAAGCCTGTCTTTCTCTTCCATGAAAAAGAGGAAATGTGAGGAGGTATGAACGAGTTATTGTAAAATTCCAAGACCTCAAGGGCAGAACAAAAAAAATAAAATATTCTGGATTCAACGCCTGTATCATACAACATGAAATAGATCACCTTGAGTGAGTTCTTTTTACCGATAAACTTATTTCTGAATAAATGTCTAAATAAACAGATTAGACTAAGAAGGGAATATTGTAGCACTTTTATTCGTCATTAATAGAGGAATGGAGGACTTTGGCAAAGAAACTCTCATTTTATGACTCTTAAAGATCATTTTTCCTCCTGTTCTTTCTTTACCTACATAACGCGAATAAGACTATTCAAGCGCCCTTAGTTGATGGCAAACTTTTCTGAAGGTGCAGGTTTCACACTTCCAGCTTTCTGCTGTTGGAGAAAATGAAGAAGAAGGAAGTGGTTTATTTTTTACAGGATCTTGATCTACTAGAAAGGTACTTTGAAAGCGCACATCTTGTTTGATTTTTTCAATCACCATATCAATATCTTTTTGTTCGATATTTCCTCCATAACTACGCATACTTGGCAAAAAGATTTCATATCCCTCGATCATTATGTCTCACAATACGGGCATTTTTTTCTTTTTCAGGAGAAGCTTGAGTGCATAAACTTTAATCTGATCCGCAATATCAATAGGATACTGAGGTTCTTTTCCTGATTTTCGGTCTAGAATAAGATAACGATTTCCTCAAAAAATGATTCAAAAATCCGGACTCGCCATAATCGAAATGTCTTTGAGATCCTGGATTTTATCAATCTCTACTTTCATAGCCTCAAAGTCAGGATTTTTTGGATTTTCAATGTATATTGCATTAGAATTTCTCATTCATTCCTGGATTCTCTCAATCCATGGAGAGGAAATCAAAGCATCTAAGTTAGCAAATACTCTCTGAATCGTCGGATTCAATCTCTCATCAATATTTTCGTGGTAAAAATGCTCACTTAATCCTCCCCAAGATTCAAAATTCAGTGTTTCATAATCTTTTTCCTTTGAAAGCTCAAATTCATATCTCATCTCCTCAGCAATTCCTTCCTTAATTTTTTGGATATTTTCTGCAGTAGCCTCTCCTGCTTGTAAGAGTTTTAAATAATCGGAAATCAAAAGATGCGTCTTCTCACCCATCCACATTTCCAGAGATTTGAGTTTTTTTGAAATGAGGGTATTTCTTCGGAGTTCTTCATTAAAGTTTTTGAGTGCAAAAGTGTAATAGTTGAGATAATATTTCTTTTCACAGTATTCTAAGAGTGTAATCCTCGTGTTTGACCAACTAAGCTTATTCATCTTCGGTTTTTTGTGAGAAGAGTAGATCATCTTTTTTAGAAAAGCATTTAAAATCAGATGCTTTGGTTTTATCATTGTAAGAATAAAAAACAAGGGAAAAACATCTCTAATGTAAAATATCTTGCATTCTCTTCTTAAATCACTAATTTGGGAGGTGAATTTTTATCACAGTTCATTGAAAAGGTAAACGATGTGAGGATATATGGATACACTACTTCAAAATATCCCTGAATGGCGAGATCAGGCAGGAGCACGACTTTCTGCATTTCCGCTTTCATTACTAAGGTGAGAATTAATCCTCCTTTTTTTGATTATTGTTTTTGTCATTATCTATCATAGGTTTAAGTCTGGAAAACAGATTTTTACGAAGTTTTGAAATAGCTTCATCCTCTTGTTTGAGATGATGTTTGAAAAGATCTATGATTTCTTTATAGAAATTATAGGAGAACATCAGAAATATTGGGTAAAATCTTTTGTAATCTGAATGTTTTTTGTGATTCTGTTTTCAAATCTTATGGGAACACTCCTTGACTTCGTAGGGATTGCGTTTCCTGGTTTGGAAGAGTGGATTATTGCTCCTACAACCGATGCTAACTTTAATATTGCGATGGCGGTGGTGTCAGTTCTTCTGATTCTTTATCTTCAGATGAAACACTTGTGAGTTGGGAAGTTTTTTTATGAATATGTTCCTCTTTTCTGAAAAAATATTATTACTATAGAAAGGGGCACGATGCCAAAACTCGTATATTATCCGCTGTGGCTGTTTGCTAAACTCTTTGATATTCTGATTTCCCTTTTTGTAGGGGCGCTTGATATTATTGGAAACTTTGCTAAAGTAATTTCGCTTTCCTTCAGATTAACAGGTAATATGATGTCGGGAACGATTTTGCTAGGGATGCTTGTGATGGGACTCAAAGCTTTGACGAGTTCAATGACGGGATTCGAGTTTCCAATCCTCTTCCCTTTGATCATCGTATTGCAAGGGGTCTTGGTTGCGATCATCCAAGCTTTTGTATTTGCACTTTTGACGGCAATCTTTATAAAGGTTGCTACTGAATAGTGTGGATCGGACTGCTAAGCTAGCATCCCCCTTTTGAAAGGAGGATTGATTGCTAGGGAAGGGCGCTTACTGGTGATGGGATTCAGAATTATTCTTGTTTTTTATCTTTTATTCTTATAGAAATTATGGGTACATTAGGGTTTGTTTGAGCAGGACTTGCTGTTGGATTGGCAGGTATTGGTGCTGGACTTGGAGAAGCGATGATCGTTGCTGCGGCACTTAATGCGATTTTAAGAAATCCAGAAATGAAAGGAAAACTGATGACTTTCATGATTCTCTTTATTGCGCTGGATGAATCAGTAGCGATCTATGGATTGATCGTAGCGTTTAAGATTTTGGCGGTAGATCCTACTGCGATGACGAATCCTTATATGTATGTTGCAGCAGGGCTTGCTATTGGGCTTCCTGGACTTGCGGTTGGAATTGGTGAAGGATATTTGGCGAAGGTTTCTATTGATAATATGGGAAAGAATCCTGAACTCAGTTCAACTTTTATGGTGCTTACCATTCTTGGACTTGCACTTGTGGAATCAGCTGCGATTTACGGATTGATTGTAGCGTTTAGATTGATTGGATAAGTCAGGATTTATTTTTGATACTAAGGCAAGATGGATATTCATTGAGATTTAGTGATTGCGGGGATCGTCAACTTTCTTGTGCTGGTGTTCCTTTTTAGGAAGCTTCTGGGAGACAAGATCGTCCAAGAAGTAGAAAAGAGGAGGACCATGCTTGAAAAACTAAAGAAAGCTGATGATGAATACAAGAAGATGATTGAATTTGCTCGTAAAGAGTCAGATTTGATTCTTGAAAAAGCAGAAAAGACAAAAAAAGATATGATTCATGAAGCACACTTAGTAGCCGAAGAGGAGAAGTCCAGGATTCTCAAGCAAGCTCATCACAAAGCGGACTTGATCGTCAGTGAAGCAAAGCAAGACACGGATAAATTGGAGAGAGAGCTGAAAGAAAGTTGGGTTGCTTCGGTAAAAGAAACCTCCAAAAAGGTCGTAAAAAGACTCCTCAAGCATGATAAGGTGCTGGAAGATGAATACTTTGGAGTTTTGGTAGAAGATTTGAAAGATTAGTAAAGAAAAAGAGATTTCCTCCTGTAGGATCTCTTTTTTCAATAGAAGGAAAAAATGGGGAATCAGGATTTTTTCATTTTTACTTGCACTCCTAGAGGAGGCTACTTGATTCAAATAAGATGAATAGCTCCAATAATGCTCAATATTGTGCTTTAGTGATACAAAAAATTTCCCAGATCTAGCTCGCTATAAAAATGGTGGCTCTGTTGCCCCGAGACCTGGAGATATTTTGTGTCTATCTGGAGGGGAGGGAAATGGCGGGCATGTTGCCATTATCATGGAAGTGACCAAGACTTACATTAAAATTGCACACCAGAACTCTGGAGATCGATGGGATGCAATTGGAGCAAGTCTTGATATGAAAGATACGAAAGTTGCCAATCCTTCAGGATATACAGTTCAAGGATGGTTGAGAATTCCTACCTATCTTAATGACCTTCCTAATCCTCCAATTCCAGAGTATCTCAAGTAAATCTCAATGTAAAAATCCTCTAGAAAAACTAGAGGATTTTTTCCTGAAATTATTCTTGAATTGAAAAGGTTCAAATTGCTTGGTATACGCTATCCACAAAGTCTCAACTTTCTCTCTTTACCTTTGAGTAACGTTTGGTATAATAATTATTCATACCTGGATCCTTACCTCCCAAAAAGCAATATTTATTATTACACCCAATAAAATCATAATAACTTCGATCGCTATATTTTTCATATTCAGACTTTGACTTAATAAAAAGTCTAAAAATAGTATAAGCTCTCGGGTTCATGGTCGCTAATGGTCAATTGAAACTTCAAATTCCATCATCTGGAAGAGTAAAATATACCGTAACATGTTGTTTTTGAATTCCTATCCCTATCTGACCGTCTACCCACTCAGATCATAGCAAAACCTGAAACCCATACACTTCATTCCTATACAAAAGTTGATCGCCAGTTTGGGTGATAACATCGCCGCTATTCGCTTCGGTAATCAAGGCTATCGCTCCGGTATAGCCTTTCGGTATCCAAGCATCTTCTTGTTCCTCTTTCAGAGTTCCCGCCTCTTGCACGGTTCAGCTCCCAGTTGCTTGATAGGATCAAGAGAGAGGGAGTTCTTCCTCTCAGGGAGCAAGTTGACTATCAGTAAAGGATCAGTTTTCTTCCTGTTGCCCTGTGTGAGTGAGTACTTCTTCCTGGGGAACGGGCTGAGAAACTAATCAACAGCCCGCCAGTAGCGAAGCCGTCGCAAGAGCAGCAAGGGAAAGGAGATAGTTCTTCATTGAATGGTCTTTACTAAGGTAAAAATTCTCAATGAATATATCAAATCCCCGATTAAAAACAAGCGTTTGATGGGAATCAGGATTTTTTTCACTTGCATCCCTAGTCCATAAAAATGTAAACAAAATGGAAAGAAAAAAAATTGCACTGCTTGCAGTGCTCGTGTGTATTCTTGTCTCAGGAAGTCTTACTTCTTGTGATAAAGATGAAAGAACGATCCCTTCTCATCAAGAGGAGGGGATTGCATCCAAAAGTGAGGGCAATGATCTTCGTTCTGAGAGTCTAAGAGCACGATTGGATGTCGTTCCATCTCAGGCTTTACGTGGAACTAATTTCCGGTTCGTCGTTTGGATATCGGGACGAAATCCTCAGAACAAAAAAGCGGCATTGCGTTTTGAATGTCCTGATGGAGTTACTCGAACCTTTGATATGCAAACGAATGGATATGATGTTGAAATGGCCTTTTTTGTACTGAACCGTTCTCTATCGCAATACGGTGAATACAGAGTATCGGCATATGAAAAGGTAGGTAATGGTTATTATAGCCTCTTCTGTAGCGAACGTGTCTTCGTATTACATCCTCCTATCCCTATGGGTGATAACTATTCATCACTTCATGTAGGAAAAGGTGTAATGTACCCTAGGAATTGTACTGCTTGGGTAACTGGCAAGGTAAACGAAATGTGGAATGCTAACTCTTTTCAGAACCTTATAGACAACCCAAGAGATGCGAAACATTGGAAGAATCACCTTGTTGCCAAAGGATATAAAGCCGATGGTAATCCTCGTGTTGGTGATATTGCTTTCTGGGAAGCAAATATCAAGGGATGTTCTGGAAGTCTTGGACACGTTGCTTTTGTCAACAATGTCTCTTCGGATGGGAATACTGTTTATATTACAGAATACAATTTCCCTTCAGGAGAACGATACGGGACTCGAACCCTAAAGAGGTATGGAGCGAGAAAGCCAAGTGAACAATTCCCTACAGCCTTCATTCACGTGCAAACAAGAAGAAATTAATACTTCATCTTGATTTTTGAACTCCCTAGAGTATATCTTTGGGGAGTTCCTTTCTTTTGAGTTTTATTTGATGCGTCTCGTCCTGATGTCAATTAAAAGGTATCGTAATCTTTTTCTCCTCTCTCTGATCGTGTGTTGAGGTCTGTTTTTTTCTGATTTCTTTGGTAAACCTTGAGCCCCATCAGCCCTGCCTCAGTATGAAGAACCCTTTACCTGAGGGATATCGACCGGCTTTCTCGAGGAACCTCGTGCACCGCTAGGAACACCAGAAGATTATGCACGTAGAATCTTTGTAGATGCTCCTGTGGCAATGGATAGTCTGCAACTTCTTCCGAAGTTCCCTCAGACGGGTGCTTTTCTGAAATTTTATGCAAATAAAGATTGGAATTTTTCCCTCTTAGCACCAAAAAACGTTGCTCCACATCAGGAAAGCTTGAGGAAAATCCCCTCTCCCTATGATCGATTTGACCTTTGGGAAGATGAAGAGTATTACTATCTGTATTCAAATAATTCTCAGGAGTATAGATGGTTTGCCAACAAAAAATCTGCATTGCCGAACTTCAAACTTCTCGGTTTCCTCGGGGAGCACGCTATTGATGCGTTTTTTGGCTATAGTGATGGGTATATCGTCATCCCTCCTTACCTCTTGCCTGTGGATCACAGCACGTATATTCCTCCCCAGACGCCTGGCAGTGGAGGAATCCAGGATCGACAGTTCTTTAGAAACGGGGGAAAGCAATACACCAACTATTTATGATCCGACAAGGACTGGGTGTATCGATGGAATAAAGATAATTCATTTGAAAACTATTTACTTCTCAGAGAAAAGAGAAAGTCTGACTTTAAACTGTTGACTGGAGAGGAAATCCAAGAATTTTTCAACCTTTCTCACATCCCGTGAGTGCAACTTCATCCGAAAAACCAAGAAAAACGCTGAACTCAGTTGACAGATGGCTATCGGAAGATTCCTAGACTTCGGCATTTCGGGGATAGAATGTATGTTTTCTGAGTGCATGGAAATTGGAGCTTTCCGATCGATGGGGCAAGTATGCAGCGAACAGAAAAAGAAATTCCTAATAATGAAAATCATACTACTTACTTTACATTGTCTGATAACAACTATTTTTATCAGCTCGTGTATCATAATGAGGGAGATTTCTATGAGCTTCAGAGGATTAAAAAGTAGAGAACGTGCGATTCTTTCACAAGTGTTAGAATATTCCATCCAAGATCGGCGTCCTAGCAATGGGAACACCCTTACTCCACCTTCTGGATATCAAGTCCAGGGATATATGAGGTGCTCTCAAGATTAAACTAAAGAGTTCTTCTTAGTCAAAATCCTCTAGTATATCTAGAGGATTTTTCTCTATAGTTGGATTTCACAGGTTTCTAGGTCAAAGGTCCCTATAATCAGATGCTTGCCATCAACCGCAGCATTGTAATTCTCCTCCGTATCAATAAGCGAAGTATTACTTCTGTAAGGCTCGATTAGTTTTCAGTTCGTCAGCCTTTTTACAATCATTTTGTCCCCCACTAGGTCATGGACGACCCGTGCAGAACGTTCTGTAATCTCATTATAACTTTCACTTTCTCTGCTCGTCCCAAGCCTATACACTTTTTTATTATCTACGACTACGATAGGATCCCAAACCTCTCACGTCATCGAAGCCTGCGTATGAAAGAAGAAATGATGTTCATTATCTCCTCGAACTTTTGGGACTAAATCTCTACAAAGCTCAATTTCTTCACACTTTGTCATCTGTTGGATCACGATTTCATCCTCATCCCCGATATAAGGGTAAATAAAGCTGAAATACGGATTATCTTGTAAGTCATGTTTTCTGATGAAGACTTCACCTTCCTTTTCTCTTGGAGAGGTGAGTTCTTGGTATTCATCACCATTGAACACACAAAGACTTTTCCCCCTCTCAGAAGAAAGTTCGTGCCATACGGGACCTGTAATGAAATTCTTCCACAAATATAGCGGCGATCGTCCTCCTCCCCCTCCTCAAAAAGGAGCATAAAAGAAATGTCCTTTCTGATACTTACAATTCCTTGCAACATCCTCTATATTTTTGATGTCCGCTTCAGGGAAAGGCTTGATATCTAAGGGTTCACAGGGAGGAATCGGCTTTTGCAATTCCGCAAGGTTTGGTGTATCACAGGTTCCTCCGGTGAATGCTTGAGTAAGCTGTTTTCCTGAATGAGAGAGCATTTCCTCTTGAGGAACTGGCTGTTGAGAAAATGAACATCCCATCACTGCCAAAGCACTTGAAAGCATCCCTAATAAAACTACTCCTTTCCTCATTTGCTACAATTCACAAGATAAATCTTTGCTGAGGATAAGGAATTCCTTGCTAAAAACAAGCGTTTGGTGGGAATCAGGATTTTTTTATAATGAAAAAAGGGAGGATTAAACTCCTCCCTCTCCTAATTAGGTTAATATTCAGTAGGTAATATGGATGCCCATAATGTGTTTTTTCACGTACTCCCTTCCTCCAGGAAGTGACATTATATCAAGATCCAATCTATTATTAGTATGACCGCAGACCTTATACCTAGGAACTCCATTATAATGCTGATTTGTGTAATGATATGAATGGGAGATAATCATTACATGTGTCTTTCTAACAGCATCATCAATATCAACTCCACCTTTTCTATTCATTGCAAGCATGTCGCCCCATTCTAGAACACAATCTCTGTCCCCATCATATCCTGCCACAAGATCCTCTTTAGAAACAATATATCCATAATGTTTCTTCATATACTCGTATAAAGCATTTGCTCCCAGCCAAGTAGATGTCCCATAATAAGGATAGCGTCCATCAAGAGTTCCCCAAGTATTATCTTTGGGGATTCCTCCGGCATAGAGACACTGAGAAACAAAATTAGCACAATCATTAGGAGAAAACTTTGCATACTCAGTATTATAAGATAATGCATGATAATACGCATAGCGCGCTGCTGCTTGTCTATCATACAATCCACTTCTTAATTGAGATAGTGGTTCTTTTTTAGGACAGAGAATACTTCCTGTCCGAGGACAGTCTCTTTCTCCAATGTAGTATTCCCAATAATCCTTTCTTGAAAGAACGTCTTTCCACATTTTAGCAGAAAAGTCATCTTGTGGATTCAGAGTAGCAAACCATGCACCTCGCAGAATTTCTCCGTCTATCCCACGACTTACGACTTGTTCCTCATAAGCAACCTTCCAATGATAAGGTGATTTCTCGTCTCTCTTGAGTTGGAGCACATAATACCTCCATCTCGTATCATTTCCACCATTTTCATCAAATGTGGGATCATCTGCAACTTCGGCTGGAGCAGGGAAATACACTCTAATGGGTATAAAGACCCATCCATTTCTCTCGCCAATGTGATTTGTCATGATATGAATATCTGGAGAAGATTGCAACACCTCTTTAGAATACCTCTTATCATCATGAAAATCCATAATCAAAAAAGCATACATGTTATTTTTTGCATACTCATTGATAAAAAGCTTATCATCAAGCCACGCTAAGCTCCTCTTCTGAAAATCCATCATAATTTTAGAAAGATAATCTCCAAAGATAAGATTTCCTATCTCATGCAACTGTTTCAGATCTTGGAGTTCTAGCTTGTTCTTGGTAAGGTATTGATTTGCCTCTTCTACCTTTCCTTCAAGGTATGAGTAATCGTGCCTCGCCTTCTCCCAAGCTAAAAGTTCTCCTGTTCCTCTAGATTGCTCTGAGGTTACAGACGGAACACGAAAAGCGTTTAAAGCAAATGTCAAGAAAATGATTGACGCGAATGGGCAACTGAAAAAAGATCCCAATTCTGAGGAGATCAACAAGTTGGACCAAGAACGTGATCAGCTTTTAACTTATTTTTTTACTAAGATTGCTGCAGAAATGCGTTCCCCTGATCAAGCGTTGGAAGCGGCAGCAACTGCATTGGGGGATTTTTTGGTAGTGTATCGTGGGATTCAAGGGAAAGCGAACCGTCAGGAAACGCAGCTGATCAAGAGTTTACTTTTGGATGCTGAGCAGGCGAAGTATACCCCACATTTACAGGCTTTAAAGCTGAATGTGGTCTTGGCAAAGCTCAAAACTGTCAATGAAAGCTATGAAAATTTACTCGACACGCGCACGAATGAAGGGCAAGCGACATTGATGATCGTGACCAAACCCCTCCGCAAGGAACTTGACGAACACTATGAAACACTCAGCACGAAACTTTTTGCTGTGAATGTAGCGAGTCCTACACCTGAGAGCGAGAAAGCGATAGCGGATTTGAATCGTTTGATCAAAGCGACGAAATTGGCTTGGAGGCAGAGGAACGGGTTGGTGCATTTGCATCCTGAGGATTTGGAAGATGAAAAAGAGAACGAAAGTGAAGGATAAGAGCTTTATTTTAGATAAAAGAAAAGGTCCTGAAGGGTATTCAGGGTCTTTTTGTGGAAAAAGGGGTGATGGTATGGCTTGCAATTATTTTTGGAAAAATTATATCTGAGTTGGCATAACAAAAAATAACGTATGGTATAAAAAAGAGAAGAACTTGGAGGTATGGCTACATTAACTCTACCTCTTTTATTCTGAATCCACATAATAATGCTCTGGAACGATATCATGACCGTGTTGAGAAAAATCTTTCATTTTCTTAAGTATGGAGGATGGAAAAAAGTGATAGGTTCCATCGTCCTTTTGCTACTCTTTGCTAATCTTTCTTTGATTAGCTTTTATACACGGTTTGCTTTTGAGGAACGGAGTCCGCAAGAAAGGAGTCAGTATTTTGGGTGTGATTACAACAAGAAGAAACGGCAACAAACGCTTTGTATTGCTTGAGATCTCCAATTGGAAGTGTTTAGAAGGTTACATACCTTAATAACATCCCCTTTTGAAACAAAGTAGGTTTAGTACCTGAAGGGTATTCAGGGTCTTTTTTTTGGAAAAAGGGGGGATGGTATGGCTTGCAATTATTTTGGGAAAAATTATATCTGAGTTGGCGTAAGTCAAGGAAAAAAGAAAGGTCCTGTCTTAAAAAGTTTGGTAGCTAAGGGAGATAAGATTGAATTTGGCTACTGTTATGCTACTACAAGAAGCTACTCTGTGATAATGAGCATGTATCTACTTCTATGAGATTGGAGTGATGCTTTTAGAGGGGCTGGTTTCTCGTCTCTCTATGTTTATTTTTGTTTTTTTGATGCCATGCATCTCAAATGATGTCAAAAGATTATTTTCTTTATCGTATGTTGTTTCTTAATCGGTAGCTTTTCTTTTTTGATTAACCCTAAAATCAGAGAACAGCTACGATTCAACATAAAAAATCTGAATTTTGGAATGCAGAGTTTGCAATCAGATTTTTCTATTTATAGTTATCTCTCTTTAGAAGATGCTGAACTTTTTTTAGAACCAGCTTCTTGATCAACTAGGATTACCATCCCTCCTAAGAAAAGTATTGCCATAGATGTGAGAGATATAGGATTCAGCACAGGATTTTCTCTTTATGATTTTTCTTCATCTACAGGATTACGAATTAATTATCGTTTCTATAAGGAGAAAGATGAAAGATTTTATCCTGATAATCAGCTTTTTTGGCCACTAGTTTTGGAATTAGAAAGGAGCTCTTGTCTCAGAGTTGACTTTTCTTATTTAGTAGCTCCGAGTAAGTGCTTAAATTTAGTTATCAATCCGGTGAGAATTTCAGAACCGCATGCTCTTGTGATCGTGAGAAATACTTCAGAACAGTCTATTTCTTTTTCTTACCTAAAAGGTTATCTTCAGGAAGAGTATCAGAAAGAGTAATTATTAAGTATTCAAAAGGTCTCTATGGAATCTCATAGAGGCCTTTTCTCTTAAATTATCGGGAGAGTATCCTAATTAGAACTTTAAGATCGTAAAATCTTTAAATAATATTCTGTCGACATCTCAACAATCTTTTCCTTCGTATCAGTCTTCATGTTTATAGGGAGTGCATTTAAGGTTGGGTAACCAATCACTTAGAACTTCATGACCAACATTAGTAAAGGTCTGTAAGAAGAAATATTTCTGATTGTATCATAATGTATCCTTTATAAAACTGTCAAATCATCGCATACTATCTCCTCATTCATTATAGATGTCCCAATACCTGTTAGTGCTCAAGATTCTTGCTCCTCCTATTTCAACGGCTCAGAGTTTCCACAAAGCTCAGGCAAGAGGACTCTCTTCTCCTGTCCATATTTTTCCATAGAGAAGAAAAGTAGTATTATCAGGCATATTATCTCTATTTATATCCACAAAGAGCTTACATCCCTTAGTCTCCTCCCCAAGTTTTACTTGAAATCCATAGACTTCATTTCTATAGATGAGAGAATCTCCCGTCTGTGTTACAATTGCCCCGCTATTTGCTTCCGTAATCACAACAATCGGAGTATCATCTCCTGTCTGTGCTTGCACTTCGGCAAGAGTGAGTTTTGCCTCCTTTCAACTCAAGATTTGTGCTGGTGAGAGTTTGGCTTCCTCTCCTGTTTGAGTACTTTTTCCACTCAAACTTTCCTGTTGGATACTATCTCCATCTCCAGTCTGCGTTTTCCTCCCATTCTCAGGAATAATTTGACATCAACTCACGAGCATTACTCCAAGTATCAAGAGGAGCGTTCAATACTTTCTCATATTTTCATCTTTATGAGTCTAAAACAAAGCTTGTGATAACTATTTTTCCAAGAAAGTCAAGGAGTTATTGTAGATGATTCTACTGCCTAGGGAACTTTCTATACTCGTAATGCTGTAAAAAGAAAGAAGGAGTCAGATTTTTTCTGATGGGTTAAAAAGGACGGAAAGTAAAAGGGGACTTATTTGTTTTTTTGTGAAAAATCGCTACAATTGAGAGCGAGATCTTTAGTTTACCAAGAAAGATGGCGTATCGTATGAAACCTTTTCTGATAAGTATTGGAAGTCTGTGTGGGATCTTTCTTTTTTTGGTGGTAAGCAATGGGCTAGGGTTTGGGATGGAAGCGTTGTTTGGAGAGCATCTGGTGGTATGGATTTTGTTTGGGATTTTTTGGTTGTTCGGGGGAGCTCTTTGGGAAACGACGGACCAGAATACGAAGTTCTTTAAAAAACTTCTTATTATCGCAGAAGTCTATCTTTTTTTATTGATTTTCGTCCAGCCGGGGATCAATCTTTCATGGTTAGAGTTTTTGGTGCTGTATGGGGCAGGGTATGGGATTGTATATGAAGTAATGACCTTTAGAGCACAAAAGTGGCAGACGGTTTTCTTTTCTAGCTGTTATGGGGTGATCGTCGCATTGATGGTCAGCATTGCAGCGTTGATGTGGTGGAGGCAGCCCTTTGATGAGGAACAATTTATCACGCAGCAATGATCGTTTCTTTTTACGGTCTTTGATGGAATGCTTGTGCCCTCCTATACGACGATTCTTTTGAAGTCTCCTATGTCTGAAAAGGTGATTTCCGCAAGCACAAAAAGTCAGACCTTCTTATTAGAAAAAAATACTGATTATCTTCTTCAATTTGCCTCTCAGCAGGAGGAGGAGCATAATTTTATCGTGCTTCAAGATCAAGAAGGAAACCTCATCAAAGTAGGACCACAATCTCAGCTCAGGTTTTCTACTCATGCTTCGAATTTTTCCCTCCAAGAGCAAAAAGGAAGGGTAGCATTTTTTGGGACAGGAGAGCTTTTGCCTCCCGAGCTAATGGAATTTAGGGATCGTTATCAAGCACGTCAAAAGGCGGCAGCCCTTGCCTTGCTCCCGGCTGGTCTCAGAAGCAATCCTACATTTCAAAGATGGAGTATCAGGTTTACCTCCTCTTTGGCGAAAGTGTTTCCGTTTTGGTATGGAGAAAATGCGAAGTATATTCAGAGCTATCTGCCGTATCTGGGATTAGCAGCGACTCAGAAGCAGCATGGAAAGGTAGACTATTCAATTCTTAAATCCACGTGAAAACTCTGAACAGAAAAAACAAACCATAAAGAAAAATATCGGGAATATTTCAAGAAATTATTCTAACTTTTTTCACAAATGATTGACATGTCTTTTTGTGTCGTTTTGTAAAAATGAGTCTGAGTTCACAAAAGTTTTATAAAATTCACAAAGTTTTAACACTGAACGTAAGTTGAGAGATCTACCTTTTAATCTACTGAAAGATGAGTTTTAGGAAACTTTTTACATTTTAGGTTTATTATTCTTATTAAATAATTTATATTCTTTTATTATTAATTAATCCTCAAGATTTAACATTGCTCTTGAAAATTGAAAGGATTTGCTTACTTTAATCTTTGTTTTATTTTCTTTGTTTCTTGCTTATGAGGGACGCTCTCAAGAGGATCGGTGTTATCACAGCTTTTGGAACTTTTTCATGATATTTGATTTATTCACTTATTATGCAGAAACCCATCGTCCTCCCAGAATATCAACATATGAATTTTGTTTTTTGTGTTGTGCTGATTGTTATTTCTCTTTATATCTCGATTTTTTACGGGATTTATCCGATTCATGTGAGGTTTAGTAGAGCTACGCTTTTTGTCTTGGGGATCTCTGCTATTGTGATGTGAAAGACCGTTTTTCTAAATGATCCTATGAATGATGCTTACTTTGGAGATTTGAGTTGTGTGGTAGGTGTGCTTCTTTTGATTATTTGACCAACGAATTTTATTATGACTTCTGGGGTTAAGAAAAAGAGAGAAGAGAAAAATTTGGAAATTATTGAGGTGTAAGTTTTTTTGCTGGGGGTAGGAAATACTCCCTTTCTATGCGGGTGTAGCTCATTTGGCTAGAGCGCTTCCTTGCCAAGGAAGAGGCAGCGGGTTCGAGTCCCGTCACTCGCTCCATGAAAAAGGGTTGATTTTGCTTGGAAAATCTTTACATTACTTCTTGCTTTTATGCCTAGGTGATGGAACTGGTAGACATGCTAGACTCAAAATCTGGTGGCTTCACGGCCGTGCGGGTTCAAGTCCCGCTCTAGGTAAAGACTAAAAAGTCTGATGTGATATTCAGATTTTTTTTGTTTTTGGAATTTGGTTCTTTAGAGTTGAGGCGAAAGCAGGAGATTTTGGAGCTATATTTTTGGGGAGTTGGGTATTTTTGAGATATTTTTAGTTGCTTTTTTCCTAAAAATAACTACATTTCATCACTGAATGAAGAAGTTTTTATTCCGTTTGTGTTTGTGAGATGGATTTGAATAAGGTAATGTTGATTGGGAGGTCTACGAGTACTTTACAGGTCAAGACTATAGAGAGCACTCAAAGTGCTGTCGTTAATTTCACTCTTGCAACCAATAGAAAGTATAAAAATAAAGAAGGAAATGTTTTGGAAGATGCTGAATATCATAGATGTGTTGCCTATGGGAATACTGCAGAAGTGCTTGGAAAGTATCTTACTAAGTGAAAGAGAATTTATCTTGAAGGGAGATTAAGAACCAGAAAATGGCAGGATACTGAAGGACATGATAGATTTTCTACAGAAGTGATTATTGATAATTTTATTTTCCTTGATACGAAAGTTTCGAACTCAGATGAAGTAGAGATTGATCCGCTCTCTGATTTTGATAGTGAGGTTCCTTTTTAAGGCTTTTTTGTATGGTTTTTCTACTTACTCTTACTTCTTAACCTTTTCATATCGATGTCTTTTAAGCAAAAAATATTAAAATTCTGACTTGAAACTTTTGATTTGATTGTTTTCTTGGTATTCGTTTTCTGAATTATTCTTTTTATTAGGTTTTTTATTGGGACTCCATACACGGTGGTCGGATCAAGTATGCTTCCAACCTTTGAATCTAATGATTGGATTATTGTGGAAAAGTTGACTCAGAGGTTTTGAACTTTAGAGAGGGGACAGGTGGTTGTTTTTGTGCCTCCTGGCAAGAATATTCCTTATATTAAGAGGATTATTGGCTTGCCTTGAGAGACAGTAAAGTTTGTAGATGATTTGACCTATATTTGTAATAATGAAGCTCCTGTTTCTGATATTCAGGCTTCGAATGGGCAGTATTGTTTCCGTTTAGATGAACCGTATCTTTATCCTGATACAAAAACGATTCCTCAGTGAGAAAAGACAGAATTTGAAGTGAGTAATGGGTATTTTGTCATGTGAGATCATAGAGGAAGCACTTCTGATTCTCTTTCTTGTTTTTCAAAGTCTTGTTATCAAGGAGCGAATTATCTTGTCCCAGATTCTCATCTTATAGGTAGAGTTTTTGTGAGATTATTCCCGACTTTTTCTCTCTTTTAGTTCTTTAGCTTGGAACTATGAAGATTTTTTCTAACTTTGATACTGAGTGGAAACAAGAGGCATACCAAGAAGCCGTAGATAAATTTTGAAAAGAGAATGTTTTTATTCTCAATAAAAGTTTTCTCTTCTTTTTTTCGAAGGTGATTGTTCCATTTTTTTGATGGACGATTATGTTTATCTTGGTTCAGTTTCCTATTTATCTTGGTCTGGAGGATTTCTGAAATATAAAGTATATTGTTACTTGAGTGCTGTTTATCATTTATCTTTTTGTTATTGCTCCGCTGTTTAAGTATTATTTGGACTATGTTATGGATTTTTCTCTCGTTACTCCCGAGTATCTTACGAGATACAATCAGACTTGACCTTTGAAGAGAGATATCAAATCTAGTTATGTAAAGAATATCAAAACTATTACTATTCAAAAAAATACTTTTTGGTACAATATTTTTGATAATTGAAACTTGATTTTTCTCTCAGAGTGAGATAGGGACAATCAGGGAGAAGTTGTTTTGCACTATATCAAAGATCCTGAGGCAAAAAAGAAAATTATAACAAAAATTATGAAGCTTTACTAATTTCCTGACGATAAAACTATGCAATTGGACAATTCACCATTGATTCCTTATGATATTTATGAATCTTCTCAAGAAATTGTTATTATTATGCCACTTTGAGGAGTGAAAAAGGAAACTATACGTCTTACTATTGAAGATTATAAGCTTATTCTTGAGGGAGAAAGGATTTTAACACCTTTAAAAGAGAATCTTTTGCCTGTGAAAGAAGAATGTTATCGAGGAAAGATTAGACAAATCATCGATCTTCCTGCTCAGATTTATTTTGATAGAATTCATAGTAAACTTTCTCCGGAAAATATTTTGTGGATTATTATTCCCAAAGCTCTTGTTCCTGAAAAGATAACGCTCCAGATTGAAGAATAGATTATTTTAGATTGTGTCTGGAAGTGCTGCTATTCGTTGTTGTTTCTTCTCTTCATAGTGTGATCTTATTTCTGAGGAGAAGATTTTTTTTAAGATTTTTTCGGAGGTATCGATCAGGCTTTGATCTATTTCTTTTTCAAAGATTTTTGGAAAAGCTTTCCATTCATAGTTATCCTCGTCTATGTTGGTTGAGAAAATATTATTTTTTAGATCTGACTTCATCTTTTTAGCAAGGGAAAAGTATTCACTTACTTTTGTTTGTTGGTATCGTGGAGAATATTCTGTTTTGAGCATATTTTCTAATCAGAAAAATGCTGATTCTCGTGCTTGGGATTTTATCTTTCGTTGTTTTTCTTGTTGTTTCCTTGTGTTTGGAATATTGCGGCAGGTGAAATTCTGATTTTTGTACTGTATGCAATATTTATTCCCTTCTTTGATTAGTTTTGTAGCTTTTATGATTGATCGTTCCTCTGTAGTGAAATCTTGTAGTATTTCATTAGTTGAAGTATAGGTTACTCCTTCTTTTGTTTTGGTATAACAAAAAGTATCAAAGATTTGATTCCCTTTTCCTATTGAAATACAGCTATTGGTATTCGGGAAATTGAAATTTCATTCGAAAATTTTTGGTTTTCCTTCTTCTAGTTCTCATGAGAGATATTTTTTATTTTTTTGGTTTATTAGGAGATAGAATCCGCTTCAGAGCAATAATTTTTGGAGGTTTTTTGTTTCATCGGTAAAAGAAATCTCGATCTTCTCTTTGCCTAGATCTTTTCCTTTTGGATTAGGTGTTATGCTGTGAATTTTAAGTATAATCCCTGATAATCATTCTTCTTCCTTTTCTCTCTGTTTACTCTCTTTTTCAGGATTTTTTTCTGTTTTCTTTTCAATATCGTAGCATGCAATGTCAAAAAGATGCTCATTTTGAACTAGAGCGATACAGCTTGATTTTGTATTTGGGAGTCAAAAGTCGCCTTTTAGGATAAGAGGATTAGTTATTTCAATATTTCATAAAAAGCTTAGGTCTTTGAATTTTCCACTTCCTTCAATATCTTCAAAGCTTAGATTCATGCCTGATACATTTTTTTCTAAGATGAAAAGTCAAAATTGATTATTAAAATCTAAAGATTTTATCATTTTTGATTTATCAAAGATAGATAGTGTGATACTTTCATTATTTTTATCACTTCCTTTGGGGTTATAGTCAATGTTTTGGATTTTTATGATTAGATTTTCAACATTATTTTTCTTTTCTTCATCTTTTAGCGAAGGATCTGGACAGTTTTTTGGATCTCGTATTCCTAGTTGTTCGTTTTTTGCTTCATGTTCTGCTTTTTTGAAAGCTTCTTGTTTTTCATAACTTTGTTTGTAGGTGTATTCTTTTGCGTATCCATTGCGGATAAGTTCTTCATTAATGAGTTTCCCGTCTAGTGATACGTATGCTAAAAGTCTTCCATATCTATCTGTATTTTCCTGTCCCTTGTCAAAAGAAAGCTCTATGGTTTTTCCTTTTAGTCTTTGTGTAAGGTAGTCTTTTGCTTCTTTTCCATAGCATTCTGTTTTTTTGTATCTTGCAGTATTACTTTCTGGTGCATCAACTCAGATAAGGCGAACAGATGCTAACTTTCCACTTTCATCTTTGTATCTTATCGTGTCGCCATCAATGATTGAGAATACTTTGATTCCTTCTAGCTTTTGGATTTCTTTTTTTTGTTTAGGAGAGATCTTATAGTAATAAGTATCCAGTATAGTCTCTTTATATTTTAAATGGACAGTGATGGTATCTCCATTTTTACTAGAATTAGGAAATCAATAACTTCCTCTAAATGTTTCAGAAATTCCTTGCTTAAGGATACCCTTTAGCTTTTTGTTTTTCCCGTTGATATCCAAGGTTAATAGACTGAGGTCTAATTCCTCACCTTTGGTCAAGATCAGTGTGATGCTTTCGTTAGTGTCTGCTCCAGGAGGATCATATTCTATATCTTCTATTTTGATTTGGTAGGTATTAATATCTATTTTTGAGGTTTCTTGATTTTTGTTTTCTGAGTTTTTATTTTTTTTGTTTTCTTCTTGGTTTTTAGGAGGCTTTTCTTGTTTATTACTTTCTGAAGTGCAGAGTTCTTCTTTTGATGGGCAGTGACAACTTCCGCCTCAATTGATGGTAATATATTCTATTTTTGGTTTGCTGTCTATTTTGAAAAAATCTAGGAATTTTTTTTCAAATCCAGGAGAAAAGTCTCCAGTTGTATTAAATATTGGAAAGCATTCTGCTTTTTCTCCTTTTGTATAGCTGGAACTTTCTGGAGCAATTGCTGTCAAAATAATGGTGTCTAAAACTTGTCGTGTTTGTCCATCAAATCATTGAATTGTTAGGTATCAAGTCTGATATAGTAGATTAAAGTCAGGATTAGATTGACTGTCGATTCCTTTGTCGTATCGTGTTTCATCTTTACTGATGATGAAGGTATTGTTTTTTTCTCGAAAATCTGTTTTTTCTAAAATTATTTCTTTGGCTAAAATCGTTCCTGTTAACATTATTTCTTTGTATTTTGCAGGAGTATCGGTCCACTTTAATTCTATGAAAGCTGGATAAGCACTATTTCCTCGGAAAATTTCTTGTACTTCTAGTCTATTGCGATCGATAAAGGTATTGCAGGATATGGGGATGTCTGTTCCTTCCTCTTCATTTGAAGGTTGTTTTTCCTTGGGTAGAGATATGTCTTTTGCATTTTCAGCTTCTGTGAAGAATGTTCATGGATTCGCAACCCGATTTTTTCACTTTATGTTTTTGATTCTGTCGATGGTTGTCCTCGTAGTTAGTCGTTTCCCGTTTATGAGCACTTTTTCGAAAGAGTTATCTTTCCCTTCCAAAAACTCTGTCCAATCTGGGTGAACGATGAGACTGTCAGTTTTATTGTTTCAATAGGTTAAAGTCAGATTTAGTCAACTTTTCCAATTGAAAGTATATTTATCTTTGATGATTTTTTTTGGAAAATTTGTTGTGAAATACTTGCTATTTTGTGTTAGGACGATAGAAGTTTCTCAGGGAATTTGTAGGTTGTAGAGCAATGGTTGTTTTTTGTTGGCTCATTTTGTTAGGCTAAGGTTTCACGTAAGCTCAATCATTCATGAAAATGTTTTATGACTAGTATTGCTTATTTCTATTCGTGTATTTGTTTTGTCAGAAAAAGCTTCTGTAATGATGAGTGTTGGTTCATCATCTTCGTTTGAAGATTCTAAATTTCAAGATGTAAGGAGAATCGGTTGATGATTTTGGATTTTTATTACATCTTTTTGAGTGTCTATTTCAGAGGTGTCTGGGCTGATGATTTCATTTTCTGGTGTGAAGTTTCAAACTTTATCTTCAATGTCTGATTCTTTGTTGTTTTCCTCTACTTTCTCTGTGCTTTTTTCATGAGATGATTTTTCCTCTTCAGAGATATTTTCACTAGAGATGTTTTCTATGTTTTCTCCAGTTATTGAATTGTTTGTTTCTGTGATATTTCAAGAAAAAGATTCTTCTGATTTCTTTTGTGATGACTGGATTTCTGGCTTATTTTCTTCTGCTCAGGTTTGTCAAACTTCCTTTGAAATCGTTGGTTCTGGATGAGATTCCATTTCTTCTGCAAAAATTGCAAGTGGCTGAAAAAAAAGCGAAGTGATCAGCAGAAATGCAAGGATTTTTTTCATTGTTCAATCTTAGAAAATAAAGGTTGACATGATATTTATATGCATACTACTGAAAAGTACTATTCTGTCAAGAAAAAAAAACTTGATTTTTTGCTTTTTTAGCTATTTTCGCTACAATTAGAGATGTATTTTATATACTTTATGTTCTTCCTATGAATTATAGATTTATTGATTTCCCTACTGATTGGTCTCAACCTAAAAACTCTAAAGATGATGTGAAGGTGCCCGAGAAGATTCTATCTTCACAACAAAAAACTAAAGAGAAGATTCAGACTTTCCCTGATAAAAAACTAGAACCAGTGGAGAAAAAGGATTCTTCATTAGATTTTGTTAAAGAATGAATAGGAAATGTAAAGACCTCTATAGAAAATTTGAACTATGGAATGAATGAGCTTGTGACTTTGGATACTCTTGATGGAACTCCGCTCAAAAATACCATGCTTTCTATATTGAAGAATCCTCAATTTGATGATTATCCTGATTTGAAAGGAAAAACTCCAGAACAAAGGTTAGAGTTTGTTTTTAGGAAGATAAATATTGCTTTAACAAAGTTTTATGCTAGAAAACTAAATATTGGGCTGACTGAACCAGTTCCAGATTATCTCAAGAAGGTAGTTATTCCTGCTACGGAATTGTTTTTGATGGAGACTTTGGCTCAAATGGGAAATGAGACGAATATCAATTTTTTGGGACAGCTATCTAGTATGGATTTTAGTAGTTTAGGGAATCTTGTAACCCAGGTAAAAGATTTTTCTGAGAAGTTTTCGTTTTCTTTTGGTCAAGGAAAGACGCTTTTGAATCTTACAGACTTTCTTGCTTTACCAAAAAATCAAGCTCAATTAAGTAAGTTGGATGATCCTTATGAATTCTATCAAAAAGTGCTCAAAAATCCTCTCTGGAAGCAAGATATTACGACAAAGGATAGTCCAAAACCGAATCAAATTTCACTTGATTCAATTACTTGGGAACAGTTTGATTTGCGTGCTCTTAAAAATAACCTTTCTAATGAAGAGAAACAGCAGGCTTTCCAAATGGGAAAGGAAAAAATGAAACAACAGCTTTGATCTATCAAAATGGTGGAAACTCCTGAGACTATGAAAAAAATGCTTTGAATTTTGGAAAAAGCGGACGGTGTTTTTCAGAATATTGATGCTCTAGGAAATAAACTACTTGATAGTATGGATATTTTGGGAAATACTTCAAAAGTTTTGAAATCAACGCTAGGATTTGATCTTTGGAAACAAATCAGGAAAACTCCAGCACTTGGAGGTGCTCTTAATGTTGTTTTAGGTTTGCTTGGTTTCTCTGGAGGAGTTGAGGGATTGGAAAGAGCTTGGAAAAAAAGGATTCTTGAGAGGGAGCTTAAACAGCCTCAGAAAGCATATATTGCTGAGTCTTATAAAAATTATATGCAGAAGAAAGCTCTTCAAGCTACTACGGCTAAAACACTTCTTGATCAACATAATCTCAAAGTGGAGGATGCTGCTCAGCAGAAGTTTGCTCTGGATATGCCTCTGATAAAAAGTCAGATTCTTCAAAAAATAGATGAAAAACCTGAACTTTTAAATCTTTCTACTTTGAAAATGACTAAAAGTCCTGTTTTTAAGGGAGAAGATTTTGTGCAAGAAGCAAAGGTTGGAGAAAAAACGAAGCTTACTCTCAAAAGAAAACTCTCTCCACAGGAAAGAGAAGCTTTTGTGGATACTTATCTCCATGAGGGATTGGAGCGTTTTGCTTCGAATCCTAAAGCCTTATCTTCACTACCTAATGCTGATACTTTAGCGTTTATTCTCCTCTCAGGGGTTGCTCTTAATAAAGATGATGTGATAGATGGTGTGGAGGCTCAGGTTTTTCTACCTGATCAGTATACTGATGCAGCACTAGCAACTCAAAATGGGCAGAATAGATATATTTATCCTAATGGATCTACTCCTATTGGAAATAATAAGCCTGTAGTGAATCCTATTTCTCCAAATCCTTCTGCAGAAAATATCAATAATTTTGTGGAGAATATTGGTCAAAAACCAGAATCTGGCAATGATATAAACAATGATCAGTTAGACGAAGCTTTGTGAAGACATGGATATAATGGAAGCCTTCTTTTCTATGATAAGGTTCCTTGAACAGAAGAACAGAAGAAAGCTTTTAAGCAGAAGATGGAGAAAATTGCAGCTGAACTGGATATTAATCCTAATCGATTAATGGCGCTGATGGCATCAGAAACCTGAGGAACATTCAATCCTGCTATAGCCAATAAATCAACATGAGCGACAGGTTTGATCCAAATTATGCCAAGTACTGCTACAAAGTTGGGAACAACAGTTTCTGCTCTTAGATCTATGTCTGCTGTTCAACAGCTTGATTATGTGAAAAAGTATTATCAACTTTCTCCAGGGAAAAAGTTTAGGTCTCTCAAAGATCTTTATCTCTATACTTTTTTCCCGATAGCTATGAATCATTCAAGTAATCCTAATTATGTTTTTCAGTCTAGTACTATTTCTGCTGCAAAACTTGCAGGGTTGCATAGAAAGTTAGCTAGAGGGAAAAGCTATATCACTATGTGAGATTTTAACTACTATATCTCTGGAATGGTGAACCAGAATGTTCCTGTAGAATTTAGGAATCAGTTTGCTTAGTTTGGCGTAGAGATATAAGAAAGTTGCCATTGATAAGGAGATAAAAAATCTGAATGAATCTTGTGATTCTTCGGATTTTTTTTAAATAAACTTTATTTTTAGTGGAAAAAACTTTTGTCGTTTCATTTAAATCATCATTTTACTCCAAGCTACAGTGGGTATTTTCAACCAAATTGTCATTTTATTCTCAATTCCAGAGTGAGTTTTTGCTGATTGCTTCTATTTTTTCGTTTGTGTTTGTAAGTTTTTTGAGTGTTTTGATGGAAAACTGCTCTATTCAGCTAAGAACAAGCCAAAGTATCAGCTATTCCTTTTTTTTATAAAAAAATCTCTTGATGAGCAAGATCAAGAGTTTTTTTGTGGTCAGATTTATTATTTTTTTTTAAAGATTTGTCAGTAAACTTACTATTGTATAAGACAGCATTGCAATCGTAATTCCTATTCAAGCTCCCATTAACGCCCAGACGGCTTTTTTCATGGCTTTTTTGTCTCCATTGGCGGTAATGAGTTGATATCCTCCGTAAACAACTACTGCGGTGCATATCACGGCAAAAGACCATCGGAGATATTTATTGATGATATTGATTCACTGAAATATTCCTTCCTCTTGGGTATCGACGACGTGTTTTTTTGGAAGATCGTCTCCTGCGTGTCCTTGAGGAACAGTAACTTTGATCCCGAAGACAGGGAGCGTGGCAAACAATGCGAGGATGACGGTGGAGAATACTTTTTTGACCATCAGGTTTGGTGTGATTAGGAAATAAAAATTGAGCTAGAGAGGTAAGAAATCTCATTAAAACTACTTGATCCAATAGAACTTACCAATCTGATAATAATTACACTAAACAGTGCAAGTAATATTCCGACTCAGATATAGAGGATGTTGTTTAGGATCTCTTTTGGATTTTCTCCTTTAGATGCTTTGATAACATAATGAACACCGTTGTAAATGATCATGGTTACTGCTAGGACCATTGTCATTCTGAGCAAGAACTTTGAAATACTAATAATAAGTGATGGTCTACGATTGATTCCTTTATTTTTTCATCAGATTTTTATTTCTGTTCCTTCTTTTAGGATGTATTTTCCTACACTCATTTTGTCAGTTCCAATACTATCTCATCCTACGATAGCATCAGATTGGATGGCAGGTGTAAATGCTTGCTCAAGAAAATCTCAGTTAGTATTTCCTGCTTGTCCTTTTGCGTATCCGATTCATAGAGAAAAGACTGCTATACTTCCTAAGCTACTAAGAATAAAACTAAAAATTCTGATTTTCTTGGCTATTGATATTTTCATATTTACATTTGAGTCAGTAAATTGTCTGCTTCAATCATTTCTTGTTCTCTTGCTTCGTTTTCTTGTGCATGGATTTTTGCCTGTGCTTGGCTATAGAGTGCAGGATTGAAACTTTGCTGGTATTTTTCGGAAATTTCTGCCTGTTTTTGGCTGTATTTTTGGTTGATTTCCTCAAGTTTTTGTTGTTCTCTTGTGAGAATATCTCTGAGTTTTTCTATTTGTTCAGGAGACATAATTGGTAAAAGATTAAACCAATTCTGTTTCTCTTCGTATTCGGCGAGAGATTTTGATTTTAGGATAAGCTGAATAAGGTTTGAGTTATTTACCAAGAAGGCTTCAGGAATTTTGAAATCTGCTGCGATTTCCCGTAGCTCTTGTGGTAGATACTGCTGTAATACTGGATTCATTGTTTGGTATTTTTATCAAAATAAATAGTTTTTAATCTTTTGGAAGAATCAGATTTTCTTTGGTGGCTGGATATCAAGTTTTCATCATTGTTTTTCTGAAGGAATCTCTATTTTGTTCAATAATGCTTCAGCTTCTTTACTTTCTTTATTGTGATATTGTTCAGCTTGCTTTTGGATGAGTGGTGTTTTTTGCATATTTTCTTTCATTTTTCTGATCTCATCCCTTGAAAGTCTTTTCATCCCACTTTTTCAGCTATAAGTTTCCTGCATTATTTCTGGGCTATACCTTATAAAATAACAGATAGATTCTGATTTAGTCTGTTTCGGTTGCTCTTTCTTCTACTCTAAAGAGATGTTTTACGTCTTTAGGGTTGATGAGTTGTCTATCTACGAGTTTCTTTGCATATTGTTTCATTGAAATCATTCCTAGTGGATTTGAGGATTCGATCGTCGAATCAACTTGTCCAAGATCCATTTTTTTGAGATTATTTTTAATAGCGACTGTGTTTAATAAGAGCTCAAAAATTCAGATTCTCGTATTGGTATCTGTTCTTTTTACGAGCATTTGAGATTGGATTCCGATGAGACTATCTGAAAGTCTGTCGGCGATACTTTGTTCCATATTTGTAGGGAAAAAGGACATGAATCTACTCAAACTATGACTTGCTGAGCTTGTGTGCAAGGTGCTAAAAACAAGGTGTCCACTTTCCGCTAGGCTAAGGACGGTTTCTGCAGTTTCAGTATCACGAATTTCTCCTACAAAAATAATATCCGGATCTTCTCTCATTACAGCTTTTAGTGCGTTTTTGAATGACCAGGTATCATGTCCTACCTGTCTCTGTGAAATACTACATTTATCTCCTTCAAAGATATATTCGATAGGGTCTTCGATCGTGATGATGTTTTCGTTTCTTGTTTTGTTGATATATTCAATCATACTTACTAGTGATGTTGATTTTCCGCTTCCTGTAGATCCTGTCACTAGGAAAAGTCCTTTTTTTTGCTGCAAGGACGTTCCTTTTTATACTTTCGGCGAGATTAGAGAACATCATCTCTTCGAGCGTTCTGATGCGAGAATTGATTTTCCTCATTACGATTCAGATTTTTCATTTTTCAAAAAAGGCATTTACTCTATAAGAGGTTCCATCTTTTCCCTCATATGCAAAATCGCTTTCTTTGTCGCAGATAAACTTATCAAAATTTTGGGCATTATTTTGAAAGAGTTGTTTGAGGATGATTTCCATGTTTTCGCTGTTGATGATCGGAATTTTTTCATCTTTGATGATATCCCCGTTCAGTCTATATGAACTTTTCCCGGAGCAGGTTAGATGAAGATCGGAAATATTCGGGTTTTCTGCAATCCCGGCTAAAATGGAATCAATATCGACTCTTACTGTGTTCATGAGCGTTGCCCTTAAAAGTAAAATACTTTATTCCAATTATATAGAGAAAGTCTTTATTTGTCAAAAAATTTAAAAGAAAATGAAAAGGGCTTTCTTTATATTTCAAAATTTGTATATTCCTCTCGGTAGGGGAGTAACCTATTTTATCTTGATGAAAAAATGAAAAAAATTACTTTTTCCTTCGTCTTTCTCGGTCTTGTTTTCTTTTTTTCTGCCTGTAGTTGGAAAAAACTTTCTCCTGAAGAGAAAAATGAAAGGATTTTGATCTGAATGAATCAAATGATTTCTTTGATAAATGAAGCTTTTGCTGGAGAAAAATCAGTCTTTTCTGGTGACGGTCAACTGCTTGCTTCTACAGAATCTCGCGTGGTAAGTCTGAATTTAACGGGTTTTATGGCGTATTCTGGTCAAGAATTTTGAGGGGAAATAGATCTTTCTCTTCAAACGAACTGATCGAGTTTGACGAACCAGCTTTTTCTTTCTTTTCTTTCCCAATTTAGGGGAGCTGATATGGATTTTTATTTTTATCCGGAAAGGTTTCGTTTTTCTCAGGGAGAGGGGAATGTAGGCTCACAATTTGTTCAATTGATTGCGAAAGATCTCCTTCATCATTGGATAAAAATTCCATGGAATAAAGATGTTTTCGTATTTCCTTCTGAGGAATTACTTTTATTTCCACAGATTTTTTCTTGATCCCTTTTTCAGCGTGAAAAATTGGAGGACTATCTTTTATATTATGGAGAGAGGATTCACTTTCTTAGCTGAAGCATAGAAAGTGAGTGAGGTGTCCGATATTTTAGGCAGACTAAATGGGATTATCTCTGAGAAGAGATTGGGTTTTCAGGAAGTTTTGATGGAAATGTCTTTAAGGGAACTTTTTCAGGGATAAAGTGAGAACCCTTGGAAATAGAACTGAAAAAAGGTAAAAATCAGATTTCTTACTCTGGAATGTGGTGAATAAATTCTTTTGAATGAAAACTTTCCTTTTTGCAGAAACAGTTTCGCTTGGAGGGTAAACGATGGAAAAATAAGGATTATGGTGAGAATATATCCTTTTTTGTTAGATTTTCTCGCTTTCAGCAAACTTGAGCTTTACAAATGATCCCAGATGACTATATTTTGCTGGAGAAATATTTAGTTGATCTAGATATTGGTTTTTAATTCTTTATGGTATGAAAAATGCAGTTAAAAAAAGTTGGAGTTTTAAGCTTTGTCTCTGTCGTTGCTATAACGCTTGCATGATGTGGGAAAGCTCCAAATCTCTCTTTTGAGGAGACTTTACAAGTGTATAATAAGCAGAATGCTATCGTAAAAGATGTATTACAGTTTATGGGCGATCAGGAGAAGCTAATTGAATCAAATCTTAAAGCACAGGTAGATGTGCAGACTGATGGAGGTAAAGGAAAAGTTATGCTTGATGTGAAGAGTGTATCTGATAATAAGACGAAAGATTCTGAGTCTGACATAGGTCTTAATCTTACTATGGATCAGAAAGATGCTCAAACAGGAGATCTTCAGAAATTGGATGCGAAAGTGAACTTGAATACGCTTTTGAAGGATTATCAGCTCTATTTTAAGCTTAATGAGTTTGTATTGGAGACAAATCCAAAAGATGCTGGGGGAATGCCGTTAGCGATGGTTGAAATGTTTAAGAATAAGTGGCTTACTTTGGATGTTCCAGAAATTAAGGAAATGCTTAGGACTTCAGCAGAGACTAATGCTAATATTTGGCAGAATGAAAAACTTTATGCAGTGAATCCAAGTTATTATACGGGAGTGGTATCAACTACTTATGAATGAAATCCTGCTTGGAAAGTAGATTTTAACCTAGAGGAAATAAAGAAAGTTGTGCTTGATTTCTATGCTTTGAATGAGACTGGAGATGTGGCTTCTACTGGAAATAAAGTATTGCTTGCTCAACAGCAGCAAATGAAAGACACTTTGGATCAGATTTTTTCTAGTATAAAATTTGAGAATACTGAAGCTTATTTTGTGATTCGTTCTGCAGATAAGGTGGATTTTGTAGTAGTAAATAGTGATTTCTCAATGGGTGATCTTAAAATGAAGCTTAGTCAGCAAGTAAAAGGTAAGGTAACGACAGCTAAGGCGATTATTCCATCTGCTAATGGGTCTTGACAAGTGGAAGTAACCCTTGTTGCTGAGAGAGCTGGTATCTGAAGTGTGAAGTTGGATCTTGTGGCTGAAAAAACTGGAAAAGATATTCAAAAAGAGCAACTCTTTAAGCTTTCATGAAAGATTTCTGCTACGTTGAGTCAAAATCGTTTCTCTCTTCAACCTCAGTTTAAGTTGACTATAGATAAAGTGGGAGTAGATGTAGACGGAGATTTCGTTGCAAAGAAAATCGCTAATCATACTTTTACTGCGCCTACTGATGCTCAGAATCTTCAAGAAATTTGGAGTGCATTAATGGGAGGTGGAGCTATGTGAGGATTTTCAGGAGAGTTTGATGATTTCTCTGGGGAAGATGTAATATCTGGAGCTGTATCTCTATCTGGAGCTACTTCTCTTTCTGGAACTACTTCTCTTTCTGGAACTACTTCTCTTTCTGGAACTGCTTGATAATTCTGTAGAGATTGATCAAAAAAAGAAGTCTGAATCTTCATAAGGTTCAGCTTTTTTTATCTCTTGACTTTTCTTTTAAAATTGATAATATTCTTTTCATAAAAAATATCTAAAAAAATAAACATTTATGGATACTTTTGCTTTAAAGGTTATTTCTGGTAGTTTCCTCTTAGGAAAGTTGCCAGACCTTGGTCGGAGAATTGCTTCTTCTGGATGTGTTCTTTCTAGTGGAGTTTTTAATTTTTCCGAGATTAAGTGTGAAGAAAAATCGAAGTTTTCCGATCTCCTGGTGGTCGTTTCCAATCTCTTTTGTCTTAGAAAAGTTCCGATTAAATCAGACTTTTTGCGAAAGGTTTCTATGGCTCTTTTGGGAGAGCGAGTGATGATTATCCTTCCTGAGCGATGGTTTCAAGATGCGAAAACTCGATATATCGTTGCTGATGATCTGGGAAGTCTCTGGCTTTTGCATTCATTAACGATAGAAATGAGTAATGTGATCCAGCATCCTGTCTCATACTTTGTCGTTCCACAGGTATGAGATTATTGTTTTGAAGGCATTCCTAGTTTGATTCTGGGAGTGTCTTTTTTTTGTGTTTGTTCTTGATTTTCTTTGTGATATCTTCATAGTGATGTTTTGTTTTATCTCTTTTGAGGGAATGGCTTTTTTGATCTGAGGGTGTTCGTTGTTTGTTTCGCCGCTTTTCTTTGGTTATTTTTTGTTCTTTTCTTGAGGAAATGTCCTTTTCGCTTCTAGTAACGAAATCTTTACTTATCAAAATTCAGGCTTGCACTCTTCTCTTTCTGCCTTCCCAAAAAAAGATGCTATTCTTCCCGATATTGAATCCTCTGCATCTTCTTGAGGAGTATTTGAGGAAATTGTTTTACTGGATGATTTCTCGGATTTAGAATAGCTGTTAAAGGAAGGGAAACAACTGGTTGAAATGATTTTCTTTTTGGTGGATCGTTTCGCTCGTTGGGGCAGATCAGAATTCTTTTTCTTTTTCGAGATTTTTGTATCGATAGGCGAAGCTTTCTGCATAATCTTCTTCTTTGCTCTTTTGTGCGTATTCGCTGAAAAATCCTTTGGAATTGCAGGTATTTTGCTCGTTATTTCGGCAAATAGTATCGAAAGCTTGGCTATTTTGGTCCCTGAAAAAATAGATGTAATGTCCTAATTCATGTGCAAGGATTTGTTTGAAATATGCTCTGTAGTTTGCTTGATATTCTGCGGTGTTGCAGGTTGCTATGTTGAGCTTTAGGGTTTTGAATTTTTTTTCCTTGGTCGTTTTGTTTTGAAGGATATGAAAAGAGCCATGTGTTTTTTTGCATCAAGGGAGATAGTTGATTTCAATATGATTATTTAGCGTTTCTTGGTCGTAGGGATTTAAAATCCCTAAACTTAGTAAATTAGTTAGTTCCTCGTGTGCGAACTGCTTCGTTTGTTCTGCAAAGGTGAGATCCAGTCATGAGAGGTTTTTTGTATTGTTTTGGACTTCTATTGTTGGTTGTTGGTTTTGAGAAGTGGAATTTTCTCTTTCCGATTGTCTAGGGTTGGCAGCTGGGTGTTCTTGTTCATTTTGGTAGTGAGAAAGATTCTGATTTCTCATCATTTCTAGGAAGGTATAACTGATGTATTTTTTTTTGCAAAATTCGGTAGGGTTTTCTGTTTGGATTTGCAATGTTGAGGTTTTGATTTTTTCAAATTCATACTTGATTCTATTAGGAGAAATCTGGCTTATTAGCTCTTGTCGAGTAGGGAGGGTTTGTTCAAGAGTACTTTTGGCTTCATCTATGCTCTCGGAGGGGCACTTTTTCTCTAAGATTTCTTTGATAAGAGGGAAATAGTTTCTGCTTGAGATTGTTGAGGATTTGATATTAAGTGTTTGTTCTACGTAGTCTTGGATTTCGAAGATAGAATCTAATTTTTGATCTGAATTTTCTGCTTTCCCTGTTCCGAAATGGAGAAGTAATGTTCCTAAGAGCAGAATAGATAGGTATTTCTTTTTCATTTTTTATTGCTATAATTAAATTTATTATTATTTTAATCATAAATTTTTTAAATGTCAATTTTTTTTACCTCATTTTTTGTAAAATAATTGAAGGATAAGTGGTGATGTTTTGATTTTTTGCTTTAGAACTGAGAAAACTTCTCTTGAATTAAGGGTGTCACATCCCTATATTAAGAGTGTTTTCAGTGGAAAAAATTTTCTTTCTAGTCAGTTTTTTCTGCTGATGTTTTGTTTTTATTTTTTATGCCTTATCATGATAGACAAGAAAATGGTCAAAGGGTTGGCGATTATGTCGCTCGTTATGTTGCAGGCTAATATCTTTGTGCCAACAGGGATTTCACTCGCGGAGGAGGGTGAGTCTGTTGCTTCAACTGTGCAAGGAGAGAGCTTGGCAATTGAGGGTTCTTCTGTACAAGAGGATTTAGCTTCTGCTTCTGCAGAGGAGAGTGTAACGGGGACGGCAGCACAGGTTTTTGAAGAAGTAGTTCAAGCTACTGGAGAAGTGGTAGATGCTTCAGGAGTGGTGATGCCATCTCTTGATGTGTTGATGTCTGCAACAGGTGCTCATATGTCTGGTGAATTGTCTGCTGGAATAATTTCTAGCTTAGGAATGAATGTAGGTGGATCTACGCTTGTGAAATATTTTACTATTGGCTCTGATGATGAGATTCCTCTTTTGATGAAAGATGATGGAACGGTTGATTATACCGGTACTAAATTGCAATATTCTGATACAACGGATTATTATTTGAATACGAAGTCTCAAGTGGTTGATTTTTGATGAGAAAAGGTGATTTTTAATGAATCATGATATCCTCTTTTTGAAAAGAAAGATTCTATTTATAGAAAAGCACTGAAGGTAATTAAGTCTGGATCTCCATCTCTTGCGTATGATCTTAGTGCAAGTTCGAAAGGAGATTGGACGAATCTTTCAGGCTCTTTTGCTAGATATGCTGAGGTAAAAAATGGGGGTAAACTTATTTGATACTTTCTTATAGCAGATGATTATGTTTATGCTCCTATTGCTGGAGAGGCTAAAATTGGAAATCAGGGATTCTTGACTTTACAAGATGCGATAGCTTCTGCTAGTGATGGGGATACTATTCTTCTTACAAAGGATATTTCTGCTGGAAAGGCGTTGTATACGACAGCAGTTGGTGCTCATGTATGTACTACTGGAAGTTCTGAGCAAAAGACTACATGACCAAAAAAGGTTACAATTGATTTTCAGTGACATACCTATAATGTTGCAGATGCGGTAGGATCTTGTGGTACTGAGTCTCAAGCAGCTCATTTTGAAAAGTGATCTGAAATAACTCTAAAAAACTGAACTTTGACCTCCAATAATGCTAATGTGCATATGATTATTCAAAATTATGCGAAGTTAACATTGGAAAACTTTACTTTGGATGCAGCGGATCTTGAGAATCTGCCTGTATATGCTATTAGTAATAACTTTTGAGAATTAAATGTTCATGGAGATTCTCATATTGATGCTGGAGAGTGAGGTGTTGCTTTTGATCTTTGGTATTGAATGAGCACTAATTATGATGGAGGAGTTTCTGTAAAGTTTGCTAGTGATTTTGTTGGAAGTGTAGATGGTGATATTGAATATGGTCCAGCTAATAGGGTACAAAGTACTCCTAACTGGGAAGAGAAGGCTGAGCTTTCTATTGAGGGAAATGGAAAATTTAATTGAACTATCAAGGCTTCTAATGGAAATGATCTCAAAAAAGCAAATATTACTGTGAAGTGAGGACTATTTTCTAATGATGTTGCCTTATATACTGTTTCTGATAAATGTTCAATCAAAAATTCTGACTCAAAATATCAGGTAAAAGATTGTCCAGTAGCAACTAAATTTTATGGTAGTAGTAATACAAGTTATATTCCTGTAGCTTTGGATGGGGAGACAATTAAGCTCAATGAAACTCATGCATTGATGAGAAAGGTGACAGGTGTGAGCTCTGAAATGACCACAGTATATAACAAGATCTTAGAATTTACTGATACCACAAATCTTCTTGAAATTAAGGAGGTAGCTGGAGAGAAGCTTTTGTTTGTAAATATGGCTACGGCTTGAGACAAACAGACAAAAGTATTTAGAGCAAAGAATGATGGAATTTTCGGAGCAGGAGCGTTTAAATCTGCAGGAGCTAGTACAGCAACAGGTACGATTATTCCTGATCTTGATCATGCGGTAGTTCCATCTTGGGTAGATAAGAATAAACCAATTAAGGCTTATCATGAGATTAAAAAAGATGCGAAAGTGATAGGATATACTCTTCAAACAGCTAAGCCTGATTATGGACGATTCTATGCACCAAAGAAATATAAAATTACCTTTAATGCTAATGGAGGAACTGCTGTAGCGCCAGTTGTACAAGGTTATGGAACTCCTGTTACAGCTCCTGCAAATCCTACAAGAAGTTGTCATAGATTTGCCGGATGGAGGCCAGCGCTTCCAGCGACTATGCCTGATGGAGATATTACTCTCACTGCTCAATGGGCCTATACTTGTTGAGGAGGAGGTTGAGGAGGATGAGGTGGTGGATCAAGTAGTTCATCATCTTCTTCAAGTAGCTCAAGTTCTTCATCTTCAAATACTGGAAATAGCATTGTTCCAGTATCAAACTTTGTAGAAAGTGTGTACTCAGGAGAGGTTACTTCTCCGCTTTGCTCTGTTGAAGGATCTCAGTTTTCAGATGAACAGAATCAAGCTTACCTTTGGGCATGTGGGAAAGATATTACGACTATTAGAGAGATTTCAGGTGCGAGACTTGATCAGCCACTTACCAGAGCAGAACTTGCAAAGATGATGTCAGTGTATGTGACTAAGATTTTAGGGAAACAGCCTGTTGTGACAGGAACTGTTCAGTATGCAGATGTTGATGAGAAATTGTGAGATTTAGCTGGATATATTCAGCTCGCTTATCAGCTCCAGATTATGGGAATTCATCATGATGGAACAGCATTGGCTCGTTTTGAACCACATAAGCTTGTTACGAGAGCTGAGTTTGCTACTGTGTTCTCTAGAGTACTTTATGGAAATAAGTATAACCAAGCTGGAGAAAACTGGGCAAAAGGTCATCTTGAAGCTCTGAAAAATGCTTGAATCCTTAAGGATACTACTCCTATGATGCAAGAAATTAGAGGTCGAGTGCTTCTCATGCTTCATAGATCTACAGCTAAGAAATAGGAAGCTTTTCGATAGACTTTTTATTAGAAAAATGCTGACTCTTTGATGGAGATCAGCTTTTTCTTTTTAGTATTTCTCTGGAAGAGGAGAATTTTTTGTCGAAAGAAAAAGAGGGCTTATCCACCCTCTTCATAAAATAAGATCATACAATGACGTCCTTCATGGGATAGTGTCCCTGGTCCCTTAACACTATATGGGATTTCATAGTCCATGACCGATTCTATAATCTTGATTTTTGGATTTTCTTTTAAAAATTCTACTATCTCTTTCCTAAGCTCATAAAGCGACTTGTTTTCAAAATCTATAACTTTTGTTGCCATATTTTCTTTTGTGTCCCTATTAAACTCTGTGTAGAGCTAGACTTTGAACACCTGGTCTATTGATTTTTTTTGTTTCTGACTTGATTGTAATGATTTCAGAGAGGATGTCAATTAAAAAGATCTTTTTTTGTATTTATGGTTTATCTGATTGAGAATTCCTTACTTTTAGAAATTTTTTGTTTTCTGTCAAACGTTATTTTTGCCTCAGTTGATGAGAAAAGTAAAAAGCACTTGCAAACTCAAAACGAAATCCTATAGTCAGGATTGTTTTATTTTACTATATCGTAGCATATGTCAGAAACAAAAAAGTATGTCTACTCTTTTTCGGAAGGAAAGGGTGTTGGGAAAGAAATCCTCTGAGGAAAGGGAGCTAATCTCGCTGAAATGGTAAGTTTAGGTTTGCCAATCCCTTCGGGATTTACTATCACGACGGAAACGTGTGATATTTACTACAAAAACAATAAAAGTTACCCAGCAGAAGTGGATCAACAGGTAGAAGAACAGCTTCAAGCGCTTGAGGCAAGAATGGGAAAGAAGCTTGGTGATGCGAATGATCCTTTGCTTGTATCTGTAAGATCTGGAGCTGCAGCTTCACTTCCAGGAATGATGGATACGGTTTTGAATCTTGGACTTAACGATGAATCTGTAGTAGGACTTGCTACCAAGACAGGAAATGAAAGGTTTGCTTGGGATTCGTATAGAAGATTTATTCAGATGTTTGGAGATGTGGTGATGGAAGTGCCTCATCATGACTTTGAAAAGGCCTTGCAATCTGTAAAAGATAAAAAAGGAGTAAAGCTGGATACAGAACTTGATACGGACGACCTCAAAGAAGTTGTAAGACTCTATAAAGAAGTCGTACAGAGAGATACAGGGAAACTTTTCCCAAATGATGGAAGACAACAGCTTAAAATGGCAATTGATGCGGTATTCAACTCATGGAATAATGAAAGAGCGATTATTTATAGAAGAATCAACGATATTAGAGGACTTCTTGGAACTGCGGTAAATGTGCAGTCTATGGTCTTTGGAAATATGGGAGAAACTTCAGGAACGGGAGTATGTTTTACAAGGAATCCTTCTACTGGGGAAAATAAATTTTACGGAGAATTTTTGATGAATGCTCAGGGAGAAGATGTGGTAGCTGGAATCAGAACTCCAGCACATATTGAGGAGCTTGATAAGGTAATGCCTGACTGCTACAAAGAGCTTTGTGATATTTATCACTTGCTGGAAAAGCATTATAAAGATATGCAAGACATGGAGTTTACGATCCAGGAAGGAAAACTTTATATTCTCCAGACAAGAAGTGGAAAAAGAACTGCTGCTGCGGCAGTGAAGATTGCTATGGATATGCTTGGAGAAGGACTTATCGATGAAAAAACGGCTGTACTTAGAGTGCAGCCAGAACAGCTTGATAGCTTGCTTCATCCTCAGCTTGATCCTCAGGCTAAAAAGGAAGCTGAACTTCTTGTGAAAGCACTTCCTGCTTCGCCAGGTGCAGCTGTGGGTCAGATCGTTTTCTCTGCTGATGTTGCTCATGATTGGAAAGAGAATGGAAAGAAAGTAGTGCTTGTGAGATTAGAAACTTCACCGGAAGATATTCAAGGAATGATTGCTTCTGAAGGAATCTTGACTGCGAGAGGAGGAATGACTTCTCATGCTGCGGTAGTTGCAAGAGGAATGGGAAAATGCTGTGTCTCTGGAGCTGGAGAGCTTGTTATTGATGAAGTTGCGAAGACGGTAACTGTTCATGGAAGGACTTTCAGAGAAGGGGATCAGATTTCTCTTGATGGAGGAACGGGAGAAGTGTTTGCTGGAGCATTAAAAGTAGTGGATCCAGAACTTAGTGGAGATTTTGAGAAATTAATGCAGTTGGCGGATAAATATAGAAAACTCTGAATTAGAACTAATGCTGATACGCCTCGCGATGCTATTACTGCTAAGAAGTTTGGTGCTGAAGGAATTGGACTTTGTAGGACTGAACATATGTTCTTTGCTGAGGAAAGAATTAAGGCAGTAAGAGAGATGATTCTTGCGGAAGATGTTGAGGGAAGAAAGAAGGCACTTGCAAAAATCCTTCCTTATCAGAAATCAGACTTTTATGAACTGATGAAAACTCTAGAAGGAATGCCAGTAACGATCAGATTTTTGGATCCTCCTTTGCATGAATTCCTTCCTCAAGAAGAAGCTCAGATTGCAGAACTTGCTACTGAACTTGGTGTGAGTGTAGAAAGACTTCATGAGAAAATAGCTTCTCTCCATGAATTTAATCCAATGCTCGGACATAGAGGATGTAGATTGGCAATTACCTTCCCTGAAATCGCAGAAATGCAGACTGAAGCTGTGATTTCTGCTGCTTGTGAACTCAAGAAAGAAGGAGTCGATGTTCATCCAGAAATTATGATTCCGCTCGTAGGATTCAAATCAGAATTAGATTTTAATGCTGACATCGTAAGAAAGGTAGCGAAAGCGACGATGGATAGATATGCTGTAGACGTGGAATACAAGCTCTGAACTATGATTGAGGTGCCAAGAGGAGCAGTAACTGCTGATAAAGTTGCAGAAACTGCGGAATTCTTCTCTTTTGGAACGAATGATCTTACTCAGATGGGACTTGGATTCTCTAGAGATGATGCAGGAAAATTTATCAAAGATTATGTAAATAAGAACATCTTTGAAAAAGATCCTTTCCAGACGCTTGATCAAGAAGGAGTAGGAGAACTTGTGAAGATCGCTGTAGAAAAAGGAAGAGCAACCAGATCAACTATCAAACTCGGAATCTGTGGAGAACACGGAGGAGATCCAGCTTCTATAGATTTCTGTCATAGAGTAGGTTTGACCTACGTAAGCTGTAGTCCGTATAGAGTGCCTATCGCAAGACTCGCTGCGGCACATGCTGCTTTGAAAAATGGATAAATGATGAAAAAATACTGAAAGCCTGATCCTCTTTGTGAGGTTGGGCTTTTTTTGTTGTGGAAGGGGTAGGCTTTTATTTAGTCGTTTCATTTTCACTTTTGTAAATCACTCAAATCGTCGTTTTGTTCCATTTTTACTTTTAAAATTTGCTTAGACTAAGCGATTTGGAGAAAAAATGAATGTTTTTTTGTGGTGGGGCTGTTTGGAAAAAGTGGAGTTTGTCAATCTATCTCCTCCAGTATTTCTGATGTCATAGGTAAAATAATCTCCCAGACAAGAGCTCGCTCCTCCAGAAAATTCAGATTTTTTCTTTTATTAAAGGTGAAATAAGGTCGAAGATATTTTCTTTTTTTGTGGTTTGAAATTTTGGGAGATATCACTATACTCGCTCACTGTATCTTTTATTTATCGTTGTATGATTATGCAAAAAAGGCAGCTTTGACAAACGAAAACTAAAAAATCATCGGGTTCGGTTTTGGTAAAATGTGGGATTCTTGTTTTTGCACTGGTAGTGATCTTGGCTCTGATTCATTCTTTTGCACATACTATTAAGGGATTCTTTGTAGGAGTTGCGAAAGAGACTGTAAAAGTAGTAAGTCAGACTGTTGGGACGCCGATGAAGACAGATGCTTACTGAAATGTGAATGTTCTTTTGGTGGGATACGGAGGCGCTGCACATGGTGGAGGGTATTTGGCGGATTCGATCATTGTTGCTTCACGAAATCCAGAATTGGGTGCAGTGAGTATGCTTTCTATTCCAAGAGATCTTTATGTGAAGAATCCACTTGGAGGTTATTCAAGGATTAATGCCGTATTTACTCAGCTTTATGGTCGCACGAAGAGTCTTCCTGAGGCTTGATCTGGCTTTGCTAGAGAATTAGAGAAGATTACAGGATTAGAGATTCCTTATTATGCGACGATTGACTTCTGAGGATTTAAGGGAGTTATTGATAGTTTAGGTGGTATTGATGTTAATGTTCCTTATGCGTTGCATGATTATGAGTTCCCAGACGCTAATCTCAAAGGATATGAACCTTTGCATGTAGAAGCTTGACTTCAGCATATGGATTGATCCTTAGCACTTAAGTATGCGAGGTCTAGGCATGCTGCTGGACATGCGTCAGATTTTGATCGTTCTTATAGGCAGCAGCTTATTGTTGAGGGAATTAAGGATAAAATGCTTTCAGGAGGAAACCTTAGCTTGGAAAAGGCAAAACAGCTCTATTCTGGTTATGTAGCAATGGTTAACACTAATGTTTCACTCAATGAGATGCTTTGGACAATTCAGTATTTAGATAGAGTAAAGCCGTTTTCTTTCTGACTTAATACTTCTTATGGATATGAGAACTTAACTACTCAGAAAGGAAGTTTTCTCTATGCGCCTCAGAGAGATTTGTTTGGTGGGGCTGCGGTCTTGCTTCCTCTTGGGGCTACGGTTAATAATGTTACTTATTATAAGGCGATTCATGAGTATGTGGATTTTATTTCCCATCTTCAGAGATTTCTCCTAGAGAATGCGAAAATTCAAGTCGATAATGGTATTGATAAAGAACTTCTTCGTTCAAATAAGATGCAAAATGTGAGGGTGGCCAGTAGAGTTGCTTCTAAGATGAAAAGGTATGGATTGGATGTGATTGCTACTGACAACACAGATCCTCTTCCACTTACGAGGCTGATTATCAATACTGAAAATGCTGAGGCAAAAGGGTTTGAAGGAACTATTACAGCGATGAGGAACTTTGTGCCGATAGATGAGGTTGTGTATAATACAGGAGTGGTGAAGAAAATCATCGACGACTATGGGAATGAAGTAGAAGTGTTTACTTGAGCGGATATTCAGCTGATTTTGGGGGCTTCTTATCTTAGTGGATTGCAAGAACAGCCTTTCAAGTATGATGAACTTGTACTTACCCATCGTGAATAATTTAGCTATTCATTATTATTATGTATTCTCTTTCTTTGCCTGAGGAGGTGTATGAGGCACTTCCTCGTCTCAGACAATTCCTTTCTTCTTTGTGATTACCGCTTGAAAAGATTCAGAATTCTGATCTTTTATGTTGCTGTTTTGTGCATAAGTCTTATGCGGCAGATTTTAAAGTGGCATATGATCATAATGAAAGACTGGAATTTGTCGGGGATGCTATGCTGGAATCGGTGATTGCGAAGAGGCTTTTCTTGGATTTCCCTGAGATGCCGGAATCTACGATGACACTTTATAAGATTGCTCTCGTAAGGGAGGAAACCCTCTGTAAAGTAGCGAAAAAAATCTGACTTCATCATCAGCTTTTTATTAGTAAAGGGGAAGAAAGGAATTGAGGCCGTGAAAAAGCCTCGATTACCTCTGATGCAATGGAAGCATTGATTGGATATCTAGCACTTGATATGGGCTATCCTATTATGGAGCAATTTGTGCTTGATAGTATCTATCCTGAGATTAATGAGGTTTCCAAAAGTCCCGTAAAATCTTATAAGACGATGGCACAGGAGCATATTCAAAAATTCTATAAACACTTGCCTGTTTATAATGATTATGATGAGAAGGTTGATGAAAAGTGAAATGTGCTTCTCTTTCGTTCTGAAATTATTGCTGAAAAAGAGGTGCTTGCGGTAGGTTTCTGAACAAACAAGAAGAAAGCTCAGGAAGATGCTGCAAAAAATTATTGTGAACAGCTGGAATAGCTTTTAAATTTTGTATATAATGACTGATCTTAAAGATCAGTTTTTTTGTTTTGTCAATATTTTCTTTTTCTTTTGTGGTGTTTTCGGTGGACTTTGTCGCTTGTTTTTGTGTTGTAAAAGGTGGCTTTATGATTGGCTTTATTGTGTTTTTGTGAGAAAAAAATAAAAAAAAAGTCACAAATAAATGTGAATTGCGTAATCATCTGTGTAAGGATGTAGGAGTGAGGTATCTCTACTCTAAAGTCAGGACAGGTGGAAGCTTTTCCTCTTTGAAGGTTGGCTTTTACTAAGCAGATGTAAGCTTTTGACAGTTTAATGTCGAAATCGTCTAACTTTGTCTTGATTTTAATCTCGGTATTGGTATTATCACTCGTAAGTTGTCTATGTGGAGACATAGAAACGGCTTACTCATACCTTGTTGATCATTAACATTCGTATTCTACTCTTGAAAAACTCAATAAATCGGAATCTTAGCTTCTGATTAGAGGAAAGATTCAGATTTATGTCTTTTGATGGTCGTAGTATTCGTTGTTCTATGGACAACAGGTAGTACGTTCTTGATGAGGAATATTCCTCACCAAAAAAGCTTTTATATTTAATTTACACAAAGAATGAACGTTAAAAAAATGTTCGCAGGCTTGACTGGAGGGTTAATGCTTGCATCAGTTCTTCCTGTATCAGTACTTGGTGCTGCTTCATACAGTCAGGAACTACAAGAAGCCTACAATTACGCTTATTCAAAGGGTATTACTACTATGAGCCCTATTGATAATGCTAATATGTACGGTGAACTTACTAGAGGTCAGTTGGCAAAAATGATCGCTAACTGGGCTGAAAAAGAAATGGGTACTAAAGTTGACACAACTGCTGTTTGCTCTTTCTCAGATGCACACACAGCTGAAGGAGACTTGGCTACTTATGTAAAGAAAGCTTGTCAGATGGGATTGATGGGTCAAGGAATTGACAAATTCAGACCAAATGATAAAGTAACAAGAGGTGAATTTGGAACAACTCTTTCTAGAGCTCTTTGGGGAGACAAGTACAATGGAGCTACTCCATTCTACAAGAATCATCTTGAGGCTTTGAGAGATGCTGGAATCATGAAAATGATTGATACTCCAAATCAAATGGAAATTAGAGGATATGTAATGCTTATGCTTATGAGATCAGTTAATGTAGTTACTCCTTCTGAATGTAAGGATCCTGCTGTGATCTTGGCTTGTGCATTAGGTGGTGATGCTTGTCCTGCTAGATGTAAGGGTAAGACACCAGATACTACTAATACAGGAACTAATAATACTGCTGGAGATTTGAATATCAACGTAGTTGATTACTCAAACCAGATTAGGTCTGTTCCTATGGTTGGTATTTCTATCTTCAACGCTATCAAATTTGATGCTACTAATGATGTAACTGTTAATTCAGTTACTTTGGAAAGAGTTGGTCTTTCTAGTAGATCAGACATCAAAGGTGTATGGTTCGAAAAGGATGGTATCGCTGTATCATCTAGAGGAACTGTAGCTACAGATGGAAAAGTTATGACTAACTTCAACAAAGGTTTCAAGGTTAAGTCATCAGAAACGCTTGATCTAGTTGTAGAACTTAGCGGAGCTAGTGCAGGTGCAGAAGTTGCATTCAAGTTTATTGGTGTAGATTCTACTGCTAAAAATGCTGTTTACAACGCTGTAACTTCAACTTATAGAACTGCTAAATATGATGTAGCTGCTCTTGATTTCTCTAATGTTGGAGGAACTGGTAATGTTGTTTACAAGCTTGGAAATCAATCAGAATTGACTTTTGGACAATTCAAGGTTTCAAATCAAGCTAAGGGAGACGATAGAAATGTATTCTTGAAATCTGTTACTTTCAGAAACAACGGAACAGCAGATCTTAACTCTCTTCTTAAGAATGTAAAGGTTATGAGAGATAATAAAGTAGTTTCTAAGTCTGTATCTATGGATGGAAGAAACATTACTATTACTCTTGAAGATACTATCAACGCAGGAAAAGCAGTTGTTTATACTGTAATGGGTGAAGTAGCTTCTCTAGAAAGAGTTGGTGATACTGTTCAACTTGAACTTAGAAAGGATAGAGACTTGGTAGCTTATGAAGCTTCAACTAAGTTTAGAACTACTATGAATAAGCCTATTCAGGATAATTCATGGATCATGAAGAATTACAAGATCGATGGTGGTAGAGTTACTCTTACAAACACTGCTGCATTCCCTAAAACAGTTGATGCAGGAAGTGGATCTTCAGATGTTGTAATCGCAGATGGTACTCTTACTGTTGCTGAACCAATCAAGCTTCCTAAGATGACTCTTGCTGTAAATAATACAGGTGTTGTTAGATCTCTTGTTCTTGAAATCGGAGGATCTAGATATGCATCTACTGTAAATGGAACAGCTTTCGTATTTGATGATGTATATGTAAATAAGACAGCTTCTATTAAGCTTATCGCTTCATTAGAATCTTTGCCTGCACATAACTCAGTTCAGTTTGTTCCAACAACAATCGGATCATTGGCTTTCGGTTCTGCAAAGGGTGAATATACTAATAACTCAGAAGATTTGAAAGGAAATGATATCGCTGGTGCAGTACAAATTGCTAAGATTAATGTAAAAGAAGCTCAATTCACTCTTAAGAATAAAGCCTCTTCAACACAGAAAGTAACATTGACTGAATCTTCTATCAAGACAATCTTTGAAGGAGAAATGGTTGCTCCTAAGGGAGATATTAATGTAACTGAGGTAACTCTTTCTGGACTTAATGCTACTGGAATTACTGATAGTTCACAAATTGATCTTCAGCTCTATATCAATGATACTCCTGTATCTAATGCAACTTATAGAAGAGGAAATAATGTTGTAACATTCTACAATGTAGGAACTGCAACAAAAACTCCTTCTAAGTTTAGAATTGATGCTACTCCTACAATGACTCAGACTGGAGACTTCGTATTTGACGTAGTTGCTAAAGGAACTGATACTAACGGAAATACTGCAACAACTGCAGCTGTAAAGACTGTTAAATTGTCTGTAGTAGAATCTTCAAATGTATCTGTAGCTAATGCTAATGCTGTTAGCCAGGTAGTGAATGAAGGATCAAATGCTGAATTGACAAAGTTCACATTGTCTGCTAGAAATGGTTCAATCAACCTTACAGGTCTTAAGTTGAACTATAGTGGTTCCGTTGCTGCATTGAATGGACAGTCTGTGACTGTTGAAGTAAATGGAAATAGCTATACTGCTACTTCAACTGCTGCTGGTGTTGAATTCACTGGTCTTAACGTTACTCTTAACGAAGGAAATCATAATGTAGTATTCACAACGAATATTAACACTGATGGTCAACCTAATGGTGTTGCTGTTGAAGTTGCATCGGCAGAAGTGAGAGGAGACTTCCCAACAGTTACTAAGGCTATTAACTCTAAGTACTTGTTCGTAAAGGCATTCCCTAAATTGTCTGTAGTAAGTGCTAAGGATAATCAGTTGGTAGTTAAAATTGAAAATGTATCTGATGATGATATTACTATCACAGATTACGTTGCTGTTAATGCTGATCCTACGTCAACAACTCTTAATGGTCAGTCTGTTACTAGTCCAATTCCTGTAGGAAAGCAGGTTACTCTTGCTAAGGGACAATCAACTGAGCTTAAGATGAATATTACTGCAGCTTGAACTCTTAAGTTGACAGGATTGAAGTATCAGGTAGTTGATGGTACGCCTTATAACTATACAGTAAATGATAACTATACAAATATCGCTACTTGGGGAACTCTTCAGGTTGTATATAAGTCATAGTATTATTGCTACTTAGGATTAAACTCTTGGCTTCGGCTGAGAGTTTTTTCTTTTTTAGAATTTTTCTGAATGAGGTTATGCTTTTCTCTTGAAAAGATGAATGATATTCACTAGAATCAGCTTAGATTTAGTTCTTGAAGTCTAAAAGAGGAGCTATATCTCATAAAATATCTATGTTAGATGCATTATCCATGAAAAAGGTTTAATTGTATTCAAGTCTTTGCTTTGATAATTGCGAATACTTATTGATAATCAGACAAATTATGCCTTTTTTGCTTTAATTCGGATGGTTTTGATTGATTTTGCATGTGGCTGTTCTCTTTTTTTACTTACTATATGATTCATTCTATGAAATTTCCCAATATAGCACGAGCAGGATGAGGAACTGGTGGTCATGTCTTCCCAATCAAAAGCCTCATCCAACATCTCCAAACTCATTCAGACCTCAAAGGAAAAATCGCTCAAATGTTCCGATTTGGAACTTCAAACAGTCTAGAACAAGAAACCTGTTCTAAACTTCAAAAAAGCATCAAAAATCTGACCTTTCATTCCATTTATTCTTGAAAACGAAGGAGAGAAAAGGGAATTTGAGCTTTGATACAAAATGTCAGAGACCTCTTTTTCTTTACGATTGGACTTTTCCAGTCAGTTTTTTATCTGTTGCGCTTCAAGATTGATGTGGTCTTCTGTAAAGGAGGGTATGTCGCATTACCCGTCGTATTTGCAGCGAAATTGCTCGGCAAGAAAATCATCGTCCACGAATCTGATGTCCATCCTTGATTAGTAAACCGTATTGCTGGAAAGTTTTCAGCCAAGAATTTTTCTGGCTTCCCGTGAGTACTTCCGAAAACCCAAGTCGTAGGACAAATCCTTTCTGAAGACTTATTACCAGCAGAAGACTCCCCTCTTTCTTGAGATAAAAAGCAGACACAAGTCCTCGTAATGTGAGGATCTCAAGGCTCAAAAAATCTCTATGAAGCTCTTGCAAAAGTATTGAAGACAACTCCAGAACTTGCAGAAATGCACTTCACGATCATTCTCGGGAAACTCAACCAAGACCTCGCTCAACTTTTTTCTGATTTTCCAAATGTGGAGACCTTGGCTTTTGTGAGTCAAAAAGAGATCGGTCGCCTCTATCTTCAGTCCGATATTGCGATCACGAGAGCAGGGACGACTTCACTTGCAGAGCAGGAACTTTTTGATCTAAAATTATTGATGATTCCAATTCCACGAACCCACGACCAGCTAGACAATGCGGAGCGATATGTTGAGCATAAAGACTGAATTCTGATCCAGCAGGATGATCCTGATTTCCTTGAGCAGCTCAAAAATCATCTACTTGCTTTGCAATCCTTCAAAAAAAGCTTATCTTCTAGGGACAGAAAAGCTGAAATCCGCAAGGCAAAGGATCAGATTTTAGAACAGATGCTTGACTAATCTCAAGCTAGCACTCTCCTTCTGAGGTAAAGCCGAAAAAGTAGAAAAGATAGCATACATCTTTCAAATCATAATGTTTCATTCTCGTAAAGCGAATCCTTACCACGAGTAAACTCGTCATCGGGAGGCGAAGCTGTGGTGATCCAGAAGACTATAATAACTGGATTGCTTCACTTCGTTCGCAATGACCGTTATCGCGAGGGAGCAAGGTGACCGTGGTGATCTAGAAAAGTATAATAACTGGATTGCTTCACTTCGTTCGCAATGACAAGCTACATTAGATTGCTTTACGATCGCTCCTAACAACTTATATCCTTTTACATCCTCATTCCCCAAATCCTATGCATATCGTTATCCTTTGAGCAGGTGGAAGCTGAATTTCCAATATCGCTCATCTTCTCCGAGATCTTGGCTATACGAATCTCATCGCCATTGATGCTCAAGAATCGCAAATTACTCAAGCCCTTCGTGAAAGAGAAATCCCAGTTATCATTGGACATGGAAAATACCAGCCGAAAGCTGATGACATTCTCATCTATTCCGAAGCGACGAAAGAAAGCCCTGAAGTCCAATCCGCTTTTCAGCTCAAAAAGGAACAGCATTTTCCGATGAAGATTTGGAATTATTTTGAATTTCTCGGAGAAGTCACGAAATACTTTCGCACAGTCGGTTTTGCGGGGACAAATGGGAAATCAAGCTCTACCTCTTTAGCGATTGTTACAGGGAAGAAACTGATTCCTGAGCTGGGACTTGGAATTGTAGGAGCGCTAGTTCCTGATCTGAATAATAAGAGTTATTACCTAAAGGAAGATAAAAAATCTGAATTTAGCCAGCTTTTTGACTTTATTTTCACAGGAAAAAAACTCCCGTATGAACTGATCAAAAAATACTATTTCTTCTTGGAAGCCTGCGAATACAAGAGGCATTTTCTCAATCTTGACCTAGAATATCTTCTGATTACCAATATTGAGCTGGATCATACCGATTATTATCGTGATTTGGCAGATTATCAGTCTGCTTTCGTGCAGATGAATCAAAAAACCAAAAATCTGACTTTCGTTTTGGATTCCTTTGAGTCTCCAGAAATTTTTTCTAAAATAAAGAAAATTCCGCTCAAGCATTGGGAATTAGACCATATCCGAGGGAAACCGACCGATTGCAATGCTTCACTGGTCGCAGGACTTTTTCAGGAACTTAATCCTGAACTTTCTTTGGAGCAGATTGAAAAGGAAATGAAGCCATTCAAAGGAATTTGGAGGAGAATGGAGTTTCTAACGACACTTCCAAGCTGAGCGAAACTCTTTTCGGACTATGGACATATCGCTTCTTCCATCCAGCTTGCTTATGAGACTTTAAGAAATCGCTTTCCAGACTACAAACTTCTCGCTATTTTTCAGCCCCATCAAACCCATAGAGTCCTGAAAGGACGATCAGATTTTCAAAAAGCACTTGCTCCGTTTGATCAGCGTGCGATTTTCTCTATCTATACTGCAAGGGAAAAGCTTTCCGATTTTGCTGATGAACCGCTTTTTCAGGAGCAGCATTTTCAGTCTTTTTCTGAGTTTTGATCCTATTTTGCCAAAGCAGTGAAAGCTGAATATCTGGATACCTTTCAGCAAGTGCAAGAACTCTTGGCTACCCCTGATGAAAAAACGATTGTCGTCGTATTTACAGCAGGGGATTTGGACTATCAACTGAGGAAAGCTTTTTTGCCTGGTTAATTAGAGGTTTTCAAAAGAAAGAAATCTGATCTCAGATTTAGAATCAAATTTTTTTGAGCTTGTATTGTCAAAAAACCTTTGTCAAAGTGCTTTCAGAGACGTTTTGGGCGAAGAAACTTTTGTTGCTTCACTAAAAACGGTGTTTTTGGTCAGATTTCAGATTGTTTTTCCTCTTTTTTGGCGATTTTTTCCAGAAGTTATAGGTTTCTGAGAATATAAGACTAAGTCTATTGGAGACCATCAAATTTTTCTCTTTAAAAAAACTGACCTGTTTGAGAGGTCAGCCTTTTTTGTTATGATAACTTATGAATAATGAGTCCACTTCTGAGTTTTGGCTCAAACCAAGTTGTCTTTGGTGGCATGATATTTCCAGTGTCAGCAATATCAATCAGCTGCTGCATACTTACTGGATAGAGTGCAAAAGCAACTTTCATCTCTCCGTTATCTACTCTTCTTTTGAGCTCTCCGAGCCCTCTAAGTCCTCCTACAAAGTCAATTCTCTTGTCGGTTCTGAGGTCTTTGATTCCGAAGAGCTGATCCAAAACGAGATTGGAAAGGATCGTCACATCAAGCACTCCAATAGGATCTTTATCATCATAGGTTCCTTCTTTCGCAGTGAGAGAGTATCGTTTATTGCCTAGATACATACTGAAATTATGAAGTGCTGTAGGTTTATATTCAGCTTCTCCTTTTTCTTCTACCGTGAAATGAGTCTGAAGTTTTTCAAGCAGTTCTGCTTCGCTCATTCCGTTGAGATCTTTCAAAACTCTATTGTAATCCATGATTTTGAGCTGATTATCTGGGAAAATTACTGCCATAAAGTAGTTAAATTCTTCCTCTCCAGTGTAATCAGGTTTCTCTGCTCTTCTCTCAAGTCCAACCCTTGCAGCAGCAGCGGTTCTGTGGTGTCCATCAGCAACATAGAGCGCAGGAACTTTCGCAAAAAGCTCGGTAATTTTAGCAACCAAAGCCTGATCATTGATCACTCGGAAATGGTGTCCGAATCCATCATCTGCCACGAAATCATATTCAGGTTTCTGATTTTTTACGACTTCTTCAATGATTCCATCAAGCTCTGCCTGTGCTGGATAGGCAAAAAACACCGGTTCTACATTTGCATCTGTAATGTTCACATGGATCATTCTATCATCCTCCTTGTCTCTACGAGTGAGTTCGTGCTTTTTGATGCTCTCATTGAAATAATCATCAATGCTGGTGCAGGCTACGAATCCGTATTGGGTTCTTTCACCCATCGTCTGCCCATAGATATAGATTTTTGGTTCAGCATCTTGAACAAGCCATCCTTTATCCTGGAATGCTTTGAAATTCTCTACGACCTTGTCATATACGGCTTGCGAATGCTCATCAGTCCCTTCTGGAAGATCAATTTCTGCCTTGGTAACATGAAGAAGAGAATACTGATTCCCTGCAGCTTCTTGTCTCGCTTCGGCTGAATTCATCACATCATAAGGGCGAGATGCAAGCTGTGCTACGATCTCTTGTGGAGGGCGAAGCCCTTTGAATGGTCTTACTACTACCATGATTTATTTGATAAGAAAATAAAAAAGAAATTCTGACTTAACCTTAGTATTTTTAATGGGAAAGTCAAGGACAATCAGATTTTTCTGTTTTTTATTTCCTTGCTGTGATGTTTCCTCTCAGGTTAGGCAGATTCTCTTTTGTGCTGGGTATTTTAGTTAGAAGTTTATGCGTTTGCATCTTCGTTATTGATTTTTTTAGCAACCACCATGATCCTCTGGGCATTGCTTCCGATAGGATAACATCCCATTAACGTGATGAAACTTCCTTTTTGGTCTTGAAATTCATGGTAATATTCAGCGACATCTTTTGGATAAATGATTTTTCTTTCGAGCATTTCATAGGTTGAAAGCTGACCATTCCATATGATTTGGATTTTTTGCCCTGCTTGGAGTTTAGGGAGGTTCCTAAAGATAAATCCGTATTCATTGTGCTTCCACCGCTCTGTTGAGCTATGCGCAAAAATAAGAGCGTTTCCTTCTGTTCATGGAGCCGGTGTAGTCGGGTATTTTACGGCACCTTGCATCAATTCCTCATCAAACTGTCCCTCTGAAAAGTCCTCTTCTCCTTTGATTGGGACATCAATAAGTGGTGTATTGACGTTGAGGTCGGGAATGATGAGTCTATTTGTCGGAGGGAGGGTATTAAATTCAATACTGTGGGTTTCTTGCTTTTTTGCAAGGAACTCTTTCATCGTAGGAGCAAGTTCTTTCTCTGTTCTAAAGCTTGCCTGGAGGCTCGTAAATTGATCTTCAAGTTCATTCAGCTTTTGTGCTTTTTGCTTAATGTGATCTTGAGATGTTGGGACTGAGGTTATTTTTAAATCCTCGGGAGCTGCTTTTGGTTCGGTCGCAAAGGTATCGTTGATTGTTAAGAGGACGAGTCTTGCGTTGGTGAAAAAGAAAAATCCTGAGAAAACAATGATGAAAAGGATAATCAGTATTTTTAACTCTGACAGGAGTTTCATTTTTGGGAGCTTGAAATCCGGAAAAGAGAATGATTTCTTGGCTGGTCTCTTAGCCTCCAAGTCAGACTCAGCTATGCTGAGCTCTCCGCTGGTATTTGCGTAAAGGTTTTCTAATTCATCAAGATGGACATACATTTTTAATTCTTACAGAGGTTAAAGTTTTCCTCTTCTATTATAGCTATTTTTTGAAATAAGAAAAAAATCTGACTTTTTATCTTCGGTCTAGATTGACAAAATCAGATTTTGAGATGTTTCTTTTTGGTGAACGGTTATTCCTTTTTGCTCAGCTGGTATATCACAGCACCAATGCTTATTATAAGCAGCAAGAACGCTCGTTTTGCCCAAGTCGCAGAAATGCTGATTGAGAAACTTCGGCTATTTGCGAGATCGGCCATTCGGAAGATAAAAGTTAAGAGCCGCTGTAGAGGGAGGGCAAGCCATAGCCAATGCGTCCAAATGACTATTATTACTGTGATAAAACCACCGATCGTGATAATTGGCACCAAAGGAACGATCAAAAGGTTGATGAGCACGCCGGTGAGGTTCGTTTCTCCAATGAAGAAAAGCAGAATGGGGAGGGTTCCGAATGTTGCTCCGAGGGTTGGGAGTCCGTAGTTCTTGAGGAAACCCGTTAAGCATGGGAGTCAGATTTTTTTCTCTTTTACCTTTGGTTTGGAAGGGGGGATTTTTTGGAATTTTGTCCGCCATTCGTTGATGAGGACAATCCCGATTACCGCTCCAAAGCTCAGGAGGAATCCTAGATCGTAAATCAGAAAGAAGGGATTATAGCAGAGCATGAGGACGAAGGCATATTTCAAAAGCCTCCAAATCTTGATTTCTCTCCCTCGGAATACTGCCAGAAGCGAAAGCACTGCCATAATTAAAGCTCTTAGCACACTGGAATCCCCACCACAGAGCAGTGCGAAGGCAACCACTCCGAGAATAATCAATCCATTTCTGATATAGAAAGGAACCCAAAAGAGCACAGCACTGAGAAATACGACAATCATCACGAGATTTCCTCCGCTGACGGCAATGATATGCACGATCCCACTGTTTACAAAGTTTTGATAGGCTCCCGTTTCTAGCTGGGACTTGTCTCCGATCAGCATTCCCAAGAGTAAGGCGGCTTCTTTGTCAGGAAATACTTTTTGGATGCGTTCTTTGAGTCGGGTTCTGGTGGTGCTTAGTTGATGATGGAGCCAAGATGCTTGGATTTTATTTTGTTGAGCTGGAAGGGAGAATTTTTCATACAGGGTTCCTGAGATGTCTTTCATTAGCAGCCGTTTATCATAGTTGAATTGGTAGTCTCGGAAGTTTTCTCCGCTTCCACTGAAAAGCAGCTCTGTGTAGAGTTTTGATGGATCGATGGTTTTTAGGGTGGCTCCTAGGTGCAGGCTTTTTCCTATCTGGTGCGGCATCTCGGTCTTGAGGAAATATGTTTTTCCGTTGTTGGTCTGGACGATGTAAGTGTTCGGTTTGGTTTGTTCAAGGATGAGGACTTCTTCCATAAAAGGAGTGAAAGCTGATTGAGGACTTTCGATCCTTAGGGGAGCAAAGGAAAAGAAGTTTGAAATCAGATTTTGATCTGTTGGTAGCAAGGTGAATGTGATTACTATACCAGCGAGTAATAAGGCTAGGCTTTCGGTCTGGAGGAGGCGTTTGCAATTTGCGAAGAAAAAGGGTTTCCTTTGTTTGCTTGCTCGCGTGATGAGGAAAAGAATCAAGACGCTCAGGAGGCAAGCTCCAGCAAAGAAAATTAGTCCTCGCTTGAGGCGGAGGAAGCTCGTAAAAGCTCGAGTAAACAATGCAAAGCTTGCAAGGAACATCGGCTTTAGGTTTAGGGGGGTAAAGTATGTTGATGAAGTGTAGGAATCGTTTTGAGTTTGTCAAGTCAAATTTTTCGAGAGATAAGAATGATTTGTTTTATCTCCTTTTGTTTTGATAGAAAAAAAACAGATTTTTATGTCTGTTTTTTTTCGTAATTTTTCGCTCTCTTTGGAAGAGGAGAGATTTTTAGTTTTGAGCGGCTGGGTTTGCTACTGGAGTAGGAGCAGGAAGGAGTTTCTGTCCCTCTGGAGTAGAGAAAATCGTTTGCAACTCCTGCACGAGTGCCCAGTGATTCTTTTCAAGCTCTGTGATTCTCTTTTCAAGTGAGCTTAGCCCTTCAGTTGTCGTTGGTTGAGAGGCTGTAGGGAGGAGGGAAGTCGTTGTCCCTGTCGTAGTTTCTTCGTCTAAGCATTTAGGCTCTCCATAACTTTCACATGCCATTCTGGTGCAAGCCGTTCCCCCATCTGCTAGCCTATTGCACGTATTGCATCCATCGTAGAAGCTTTTGCAGGTCGCTGGGATACTGGCTGCTAGGTCTGTAGTAGGAGTTTCATCTGTAGTAGCGGTTTCGTTGTTGCCTGTAGCGGTATCGCAATTCGGACAACTACAATTATATTGATTGTGGAGGTGCTGTATTACGGATTCTGGTAAAGCACTTGCTCGGTAGTGAACGAGATTGCTATTTTCACTCTCGTAGCTCGTGTAGATTCGGAGTCCCACGAGTGCCAAAAGCCAAAAAAGGGTTGTTCGTAAAATAGTTTTGAATACTTTCATGATTTTGTATAAATAAACTAAAAGTTAAGTAAACAGTTTGAACTCCTGAGGAAGTGGAGCCTCGAAGGCAATCAGCTTTCCATTAAGGTTTTTGAACTGATACTTCCAACAGTGTAATAATTGTCTCTGAATATGCAAGCTTTTGTATACAATTCTATTGATTGCGGGTTTCCCATAGATGAGATCCCCCAAAACAGGGAAGTTTTCGCTTGCAAGGTGGATTCTGATTTGGTGCATTCTGCCGCTATAGAGCTTTACCCTAACGAGGGAAATCTGACCGAGCTCGTTATGCTGGAGCGTTTTTTCACATCGGCATTCAGTTTTTGAATCTAGTCCATCACTTTCGTTGAGCTGCATCTGAGCTCTGGCGAATTTGGCGTTGTAGGTTTTTTCTATTGCTTTATCAATGATGAGATGCTTGGGAAATTTTCCTGCGACTCGAGTCAGGTATTCTTTGCTGATTGCCCTTTCTCTGATGATTTGGTTGATGTATTGCAGGGACTCGTAGGTTTTTGCGGCGATGAGCACTCCAGAGGTATCTTTATCTAGTCTGTATCAAAAAGAAGGTTTAAAAGTCTGATTTTTTTCCTCTTTTTGTCCCTCGCTTCTCGAGCAGTATCGTTCTAAGTAATCGTTCATACAGAGATCGTTTCGGTGTTTATTACTTGGGTGGAGGACGATCCCAGCCGGCTTATTAAATGCGAGTCGCTGCTCGTCTTCGTAGAGAATCCATGATTTTATGAGTTTAGGGTCTAGCTTTTGGAATTTTTTTTCTTTTTGCGTGAGCATAAGCTGTTTGTCTTTGGTGCCGAGGAGTGATTCTGGGAGCTCAAGCAGGTCGCCTTCATGGAGTCTGGTTTCTTCTTTTGTTTTTTTTCCATTTACTTTGATTTCTCCTTTACGAATCCAAGAATAGATGTCAGAAAGTCTGACCTGCGGATAGGGTTTACAAAATTTTCTCAGGAAGCGATCGCATCTCTGGTTGGCTTGGTCGGGAGTAATTATTAGTTCCATTGGAGCGGCGAGGATAAAGCGTTTCCTAGTATAGAAAAAACGCAAAAAACTTCAAGTTTAAAAAAACTGATCCTAGGATCAGTTCTTGAGCGGGATTATTTCTTGGTTTTGTGCGGTGGATTCTCTCTATAAGCATAGAGTCCTTTTTCGATTTTCTCAAAGGTAGTATTGTATTTTTGGAGATTAAGGAGGATGGTGTTTTGACTCACCATTTTTTCCCTTAGCACTTCTCTCGCTAATTCCTTTACGGTCATCGGCTTTTGAAATCTTTTGAAAACTCTTATGAGAATATCTTTTACATTTCCTCCTTCATATCCTCGCTCTTTGAGTCCGTAAAGTCCTAGTCCTAAGTTTACAAAGATATCGTTGTGTTTGACGAGGTCGTTGTGTACGGAATTTGCTTTGATAGAGCATTCTGGGAATCGCTCCATAATTTTTGCTGGGAGCTCTTGGTAATGGATTGGTTTATTGAGTCTTCTCATCGTATAAAGGATCTTGAGCTTCAGAGTTTTTGGATAGATGTCTGGAAATTCTACCAGTCCAATCTTTCCATCCAAAATTGCCATACCTTTTACAATGCGGAAAAAGTTGATATAGAAGAGATCGTTTTTTAGGAAGCTGATGTCTCTGAATTCCTCTAGGAACTCCTCCTTTAGGGTCCCGATAAATTCGTAGAGGTCTTGGCTTTCCTTTCTCTTTGTGAAGTAATTTTGGCTAAAAATTACCATTTTTGTGAGAAGGTCCTCGTAAAGAGGCTCGATATAGAATGCTTTAGAGAGATATTTATTTCTTTTGAGATAGGTGATATCAAAGTCGGAAATCAGAATTAATTTTAATTCTGGCTTTGAAAACTTTTGAACATTTTTGTTCATTATCTTTGCAAGGAGGGTGTCCTCGTCAAGAAGTCCTCCATGGATCTCAAGGATTTTTTTGGTTTCCTCTAGGACGTTGATGTATGCTTCATTTCCTACAATGAGTCTTCTGAATCTCATTAATGCTTGAGTTTCGATTTGTCTTACCCTTTCTCTCGTGAGAGAGTATTCTCTACCGATTTTCTGAAGAGGAACCTCTTTTTCTCCATCAAGTCAAAACTTTCTCATTAAAACCAGCCTTTCCTTTGGTTTGCAGTAAATGAGAACATCTTTTAATTGAGTAGTTTCTATATCTACTTTAATTTTTTTATCATCCATTTTTGCAATAAATCCCATGGAGTATGTACTAATAATAAATTGATTTATGTATTTTCTTACCTATTGGAGTATAAGAAATGGAAAGAGAATGTCAAGTATTTTATTCTGCTTTAAAAATACTTGGTTGGAAAGGAAGAACGTCTCGCTTTTTTCAGGTTTTTTCTATTTAATGGGAAAGCTATAAGTTAGCTATGGTTAATTTTGCTCCTTTTGTGCAACCATTGAGGGCGTTTTTAGACTCTGCCATATTTCGATAGTCACCTTTTACATCCGCGGTTTTTACGGTAATTCGTTCTTCTGCCAAGCCTTTCATTACCATATAAATATCACAGCTGACGGTCATGTTTGGATTGGTATTAGAGCCGATCATTCTTTTTTCAAGCGCTGTTTTCATATAAGGATAATTACTGTCGGAAGTTGCTATGTGCGTGAATTTTGTATTTTTCTTGGTAGAAAGAGGAATATTATTTGTTTTGATAAGATGTTTAATGGCATCAATCATCGTCACTTCTTGGTTTGGGCTGCTTGTCTTGTTTGAAGGCTGGTTTGAAGTCTGGGTTTGTGTGCTGGCGGGAGTTTGCGTATTTGTAGGTTCTTCAATTCTTTGGACGCTCAGTCCTGTAGAGAGATTTGTTCCGCTAGGTAAAGAAGATGATTCTCCTGAAGTGAGGCCATTGGCTTCTGCACCTGTTTCGTTGATGATTAGGTTGATATTGTCTCCAGATAAACTTTGCTCTCCAGAGAGAGAGGATGCCTCTCCGCTAAGATTCATCTCAATAACACTTCCTGTTCCTTCAAAGAGGAAGTCTGAATCAATTTCTCATTCGTTACTTCCTGTTTTGGTATTGGTAATTTCTTTTCCCTTGACTCCTTCAATGAAGGTAGATATTCAGTTTTTTATGTCTTCTATTTTTTCATATTTACCTCAGAAAAGAGATGCTGCGAGTCCTCCACATCCAAGAACGAAAAAAATGAGGAAATATAATGAAGAAAAAATTGGTCTCTCTCGCAGTTGAGTAGTTCTGAAAATGACCATGATTGCGATCATCATTCCGATAGCTCAGATGAATATTGCAATGAAGTCGAAGATTTCTCGAAACATATAGAGAATCCCTCAAAATCCTATAAGTGCAAGAAGAAAGATTAAAAGACGATAAAGTCCTCCTCCGTTTGTCGTCTGATTTTTTCTGTTACGGTTTATTCTGGTTTTGATATACTCAATATTGGATTCAGATAGTGTAGGAGAGGATTCTTCCTTTTTTTCTTTTGTAAAAAAATCTTTTTCTCCTGTTTCCTTAGGAGTTGGGTGTTTTTCTCTTATGAGAGGAGCTTCTCATTTTTGCTCCGATCTTTGTTTAGATTGTTTTCGTAAAAGGGCAAGGAAAATCGTTGCTACAAAGTTGATTCCTCCTAAAATGTATCGCCTTGGTTCGTTTCGTAGAAGCCCTATGATAATCGCTTGGATTACAAAGATATAGATGATGCTTATTATGATTGGTCACTTTTTCGTCATGTTTGTCATGAATTATCTCAGTAAATAAAATTCTGTTTCTTTGATAGCAATTTCTTTTCTTTTTTCAAGCGGAGATGTGATAAAAACGAGGGAACTTGTAGAAAAAATTTTAGTGAGTGTTTTTGTTTTAGAATGTTTGATGATTTTCCCTTGAAATTCCCCTTTTTATTGATAGGAATGGGATGTTTTTTGTGACTTAGAAGATGTTCGGCCTTTGGGGGCTGTTGTGAATGATGTGATTGTGCTTTTTTTGAGGCACGTTAAATATTATATTGCTAACAATAATGCTTATGTCTACTAGGAAAAAAATCTGATTTTGGTGTCTTGCAGTTTTGCTTGTTTCAGCAGTTTCTATTTCCTTTATGTTTTCTCATATCTCTCGTCAGCGTCAGTATTTTCTGGCTGTTATTCCTCATTTTATGATTGACCCGGTAAAAGTTCAGGATTTTTATTCTTTTTTGAAGGGGCGTTTTTTTTCTTCTCATGAAATGCCAGATCAAATTGTTCTTCTCTCTCCTAATCATTTTTTTCTGAAACAAAAACGTGTAGAAGGACTTTGTACTTCTGGGAGAATTAGGTTTAAAGATCAGACTATTGTTGCTACTCCTTTTGTAGATGATCGTATTGCTTGTGATGGAGGGGTTTTTTATGAGAAAGGAGGGCAAACTTATACGAAAGATCATGGTTTGGGAGAGCATTTTCGCTGGATTAATCAGTTTTTCCCTACTGTTAGAACTGTTTATCTTTTTGCTCTTCCTACACATCATATGGAGCACGCTTCTTGGCTGGCTGAAAATATCCAAAAACTGCCCTGAAAAACTTTGGTTATTGCTTCTGTGGATTTTTCTCATTATTTGTCAGAGGTTATTGCCGAGCAACATGATCAGGTTTCTTTTTCTGTTTTGCAGCAAAGTGAGGCTTTTGATCAACTAAGGAGATTGGATGTTGATTGCCCTGCCTGTTTGGGGGTTGTTTATCGTCTTGCTTCAAGGGAGAAAATTAGGGTCCATCAGCGATGGAGAGATTCATCAAGCACTATCGTTGGAAAGGATCTGATGGATCAAAACACTTCTAGGCAATTCCTTTGGCGGGAGTAGTGTTTCTGCATCTTTTTCGCGTGTTTTTAGTGTCGGTTTGTATAATCCTTCTAGGATGATTGTTGCTTTCCCTTTGGGTAGATTTTCATAGATTCGTTTTTGGTATAGTCATGGAACTCTAATGCAGCCATGCGATCTTGGTAGACCATCACTTCGTCCTTGGTGAAGAAAAATGCCTTTGAAAACTTTTAGAGAATAGGGCATAGGTGCATTTTGATAGGTTTTTGATTTGCGGAAAATCTCATCGTGGCTCAGTGGGTATCTTCATTCTGGAGTCTGGTTTCCTCTTGTGCCTATCGAAACGTGAGTTGCTAATTTTAGTGTTCAGTTTTCGTAATAGGCGAGGGCGTTTCTTCCGTTTTCGCACTGGCTGACTATGAGTATATGATCAGATTTATTTTGTTTGTATTTAGAAAAAGGAGCTTTTTCAAGTAATGTATCAAGTTGTTCGGGAAAATACTTTTTTAATTCTGGATAGATCAAAGGTATATCGATGCTGTCGCTATTCTTGAATAAAGTTTTGTGTGTAAAATGTGCTGAGTCTCTGGCGTATTTCTCTCTTAATATCTCATAAACTTGTGATACTCCGATTAAGTTCCCATAAGTAGGATCTATTATTTTTTTGCTTCATCGCAGCGGTCCTCCTTCTAATTCAGAAATTTTTACTTTGTAACTTTGTGAGGAATCTATCTTCTCTGAAAGACTTTCTTCTTGATTTAATTCGAGGGGTTCTTTGGTAGATAAGATATTTAGAATTTCTTTTTTTGTATTTTCTGGATATATTGTTTCCAGTGCTAGTGTGCTTATTCATAGCATCAGTGTGTTTTTCCATCTCATCAAGATAACTCCTGAAAAATAAAAAAAGATTTGGAATTATAATTTTCTACAATAAAAAAGCAATCTCTACTTTTCTTTTTTTGTTTTCTTTACTTTTTGTGTTGACAATTTTTTGTATAGCAGAATGTGTTTATAATTCCTTGACTTTTACTTTCTGAACTTTATACTCTCTGGGTTAATAAAAAAATAAGGAAATGAAAAGAAAATACTCAGATTTTTTCAGTACTATCCTCTTGTTGACTGTGATGATACTCTTGTTTGTGTTCGTCTTGTCGCTTCTTTCTGGAGTTTGGTATTGTATCGGGATTAGTTTTGCTGCTTATGTCCTTGCACTTGGGGGATCTCATTATTATGAGTTCTCCTATGATGAACAACTTCCCCCTGAATAGGGGGATTTTTTTGTGTTGCAAAGTTGAGTATTTTTTTGAGATTGGGTTGTTTTTTTGAGGAATCTTATTATATTGGAGGTCTTTTATCCTTGTACGAAATATGGTAAGGAAGCAAGTATTGGTGATTTTGATGGTGGTTTTCTTTTTGTGATGAGCGGGGCTACTTTTTGCCAGTTCGTTTGGTGGCTTAAATGTTGAATCATTTATTATGGGTTCCTGAGCGGTTGTTCCTACTCAAAAAGAAGAGGTGCAACTTGTGGTTGGTGGAGATATTATGCTTTCCAGGAATATTGGATGGCTCGCTAAACAAGAGGGGTATGATCGTACCTTTTCTGGTCAGAATTTTAATCCTCTTTCTCAATTTTCGTGCTATGCATCAGGAGACTGTCTGCTCTACTTTAATCTTGAAAGTATGTTTTCTGCTAAAGATAATGATCAGCCAAAGTGAGGCTTTACCTTTCGTGCTAATACAGGGAATATTCAGACCTTGCTTGATTTGCAGGGGAATAATGAATTAGTGCTTTCACTCGCGAACAACCACACTAATAATGCCTGAGGAGATGGGGTAGAGTTGACTCGTCATCGACTTTTTGCTCATGAAATTGGACATTTTGGAGCGGGGAATACTACCTGAGAAGCAGAGGCTTTTTATTCTACTCGTAAAGGGTGAATAAAATTTTGTTTTCAGGCTTTTTCTTATGATTGAACTCATTGAATTTACGGAAAGAAAAGTCTCGCCTGGAATCCGCTTAGTTTGCCTTTGATGACCGGGACTCTTCATGCGATGGAGCAAGATCTTTGTGATGTGAAGGTATTAGGGCTTCATTGGTGAGCAGAATATCGCTTCCAACCAGCGAAATGGCAGATTCTCTTGGCACATCAGCTTGTCGATGCTGGAGCTGATATTCTTCTTGGAGGGCATTCACATGTTCCTTGACCTTATGAAATTTATAATGGGAAGCCGATTTTTTATTCTTTTGGAAATTTTCTTTTTGATCAAGATTGGGGTATGAAGACCTCTGTTGGAGAATTTGATTTTAACTATGATCATGAACTGAAGAAAAATAGAGTGCCTACGTATATTCCCCTATTGGCTTCATTACAAATTGAAAAAAATGGCACAGGTATTCAGATTTCTTTTCTTCTATTTAAAATGGCTCGTTTATATAAAGGAATTTTCTCTCCTCTTGATGATGAAACCTTTTCTGGTATGATGGAGCATTTGGTGTTAAAATAGTCGGTGAAGTCTGTCTTTTGATTTTGTTTGAGTTTTAGGTGTCTTGCTTCTTGATTGACTTTTATTTTGTGACCTTTGGATTTATGTGGTGATTCTTTGTCTTGTGATTTGTTGTAGGGGCGGTGATCGCGTATTTGATCTTTTATTTTCAGTATGAGAGAAGGGAAATCGTTTCAGAACTTAGGAAAAACCTAAAGTCTGCGAATGAACAGCTCAGTTATCTTCAAGAAGAGATGGAGGAATTGACCGCGCAAAACCAGCTTTTTAGAGAAAAAACTACAGAGCTTCTTGAGAAAAATGATGAACTTTCAGATGTTGTGGCAGAATTGGGAAAGTATTATGTTCATATGAAAAAAGCTTCCGAAAAGACGGCAGAACTTTCTAAATTTTTGCATGAGCCAGATGAGGAGATGGCACTAAAACTTGATACTTTGTCAGAAAAAACTGAACGTCAGTTGAAGAACCGTTTTTTTTAATTAAAAATTCTTCTTTTCTCTTGACTATTTATCAATTATTCCTATTCTTTGGCGTAGTCTTTTATTTTTTCTGCCTAGAGAGATGGATATCAAGGAGATAGATAGTTTTTTTGACTATCAGATGACCTTTGATGAGGATCCGCTTTTTTTGCTTGATGAAATTATTGCTCAGGCTTCGGCTTATCTTCCAGCGGAACAGATTGAAGGTATTAAACATGCCTACACTTTTACTAAAGCTGCTCATGCTGGAGTTAAGAGGCTTTCTGGAGAGCCGTATATTGTTCATCCTCTCAAGGCAACGCTTTTTTTGATGGAGCTTAAGCCCGACTTGGCATCTATTCAAGCTTGTATTATGCATGATGTGATTGAGGATACGCCGATAACTCGTGAAGAAGTTGCTGCTGAGTTTACCGAAGAAGTAGCGGAAATTTGTGAGGGCTTGGTTAAGGTTTCAAAAGTGAGGTATCAAGGAGAAGATCGTCATTTGGAAACGATAAAAAAGACCTTTCTTGCGATGGCGAAAGATTTGAGGGTGATTTTTGTGAAATTGGCTGATCGTATTCACAATATTCAGACTTTGCAGTTTCATCCAGAAGAGAGGAAAAGGCTGAAAATTGCAGAAGAAACCCTAAAAATTTATGTTCCAATTGCTAAAAGGCTCGGGCTTTATCATTTTCAACTTCTTCTCGAAAATGGTTCTTTTGCTACAATGAAACCGGAGGAGTTTTCCAGAATTTTGAGCTATTTGAAAAAATATTTTCGTGAGGGAGAAAAATATACTGAAAAGGGAGTGAAAATGCTGACAGGGATGCTTCATAGAGAAGGGGTTCAGAATTTTGAGGTCAAGGGAAGAATTAAAAGTCCCTATAGAGTATATGAAAAGCTTTCCAAAAAGTATCATGAGACCGATATTTCTACAGTAATGGACCTGCTTGCTTTTAGGGTGATTACTCAGAGTGTCGGTGATTGTTATATGGTTTTGGGGGTTATCCATAAGTATTATATTCCACTTATCAAGAAGATTAAGGATTATATTGCGATTCCAAAATTTAATGGTTATAAGAGTATTCATACGACTGTTTTGGGGATGTTTCGTTTTCCTGTGGAAATTCAGATTAGAACTCAGGAAATGGATGATGTGGCGGAGTTTGGAGTCGCTGCTCATTTTGCTTATGCAGAAAGCAATGCTCCAACCGTGGTTTCTGAGCAGCAGTGAGTTTGGATTCAAAAGCTTCAAAAAATTGTAGCAGATTATACCGATTCTTCTGAGAAAGAAAGGTTCAAATCTCAACTTAATATTGAGGTCCTTGATAAAACGATATTTATTTATACTCCGAGATGAGATATTAAGGAGCTTCCTGCTGGATCAACGGTTTTAGATTTTGCTTTTAGTATTCATAGTGCTATTGGGCTCAGATTTAAGAATGCTATTGTGAATTGACAGATTAGACCGATTAGCTATAAGCTAAAAACTTGAGATATTGTGAATATCAATACCTTTAAGAATAGGTATGTTGCGAATAAACACTGGATCGAGTTTTTGCGTACTCCATCAGCGAAAAATCAGCTTTTAAAATATTTAAAAACTATAGAAAGAGAGACGCGTTTGGAGGAAGCAATTGATGGTTTGAATCAGTATTTGAAAGATTTGGGTCTTCCTTTGTTTCGTAGTGATAGAGATAAAATTCAGAAGCTGGATAATCCTCTTGAGGTGGAGAGGAGAATTTTGATGGTATTGGATAAGCAAGATTCCTATGGAAATATTGTAAGGCTGGCTTATCCTGATTTGCTTAAAAATGTCGTGGAGAAAAGTATTATCAAAGATACTGGTGAACTCAAAGATACTTTAAAACAAGAGATAAAAAATCAGAATTCAACTTTTTCTGTTATTGTTGATAATGACAAGCATTTGAATTGTACTTTTTGTCCGGAGTGTCATCCTAAGCTTGGAGATAAAATTATCGCAAAATCTACTAGACAAGGAATTAAAGTTCACACTATGCATTGTAAAGCGTTAAAGACTATTTCGTTTGATTCTCTTTTGGAAGCTCATTGGGACCAAGAAAGCCCAAATCGTTATCACTTTGAGCTTCAGATCAAATTTTCTTCTCGTGAATTGACGATCGTAGATTTTCTTCAGGTTTTTTCTCAATTTAATGTGCAACTTTCAGAAATATCTATAAAGCACGGAGAAAATGCAGAGGTGATTGTTGATTTTGAATTGCAAGTGGATAATCCATCAAAAATCGCTTTTATTCTGAAAGACTTGAAAAAGTTTTCCCTTTCCTTAGAAATTCTGAAGAAAGTGATTTTTTAGTTTTTATTTTTTTGTTAATATTATGGCAAGTGGTTTTCTCTCAGGAGTAGAACAAATAGCGGAAAGCTGAGTTCTCCCTTCGACTGTCGATACTGTGGCGCAGCCGCAGGTGCCGGAATTGGTAGTTAATCTTCAAAATCTTGGTTCTTGAACTGATGTAGTTTCACTTATTTCTGGATATTTCCCATTTTTGGCTTCTTATTCAGATCGTGAGCTTTTGCTGTTTGGTTTTTTGGCTTTGGTTTGGCTTCTTTGTGCAATTTGGACCTTGAGAGACGCGATGGCGAGGAGTGATAGTTCATTTTACCAGTTTTTTTCTGTCCTTTTGATAGTTGTTTTGACTCCGGTTTTTGGTTTGCCGCTTTATTTGGCGTTTCGTCCTTTGGTATATAAACGAGAAAAAGGATCTTGGAGAGAGGCTTTGGAACAGACTGTTGTGGAATGTCCTCATTGTCGCGGTTTGAATTCTGTTTCTCATAAGGTTTGTGCTCGGTGTGGGGAAGCTTTGTGTGTTGAGTGTAGGGAATGTCATAGTAAATATTCTGGTCAGTTTGCATATTGTCCTGAATGTGGAGCTCCAAATATTGAATAGTTGCTTTTTGGGGAGATTTGGAGTATACTTAGATCGTTTAGTGGATAAAAAACTAATAAAATGTTGTTGATGCTTGTGTTGATCGTAATGATTATTAGTTTAGCACTTTTGCTGGCACTTTTTTCTGGTTTTTTTCCTTTTGTGCGTACATATGGGAATGTAATGCAATATTCGACTGCCTATTATGGGGCAATTTCTGCTCTTGAAAGGTGAGTCTTGGCCGTAAGGTATACCGGGCCTGGTTTTGATTGAGAGTCTGGTCGAGAGAGTACTGCTGCAGCTGTTCTTGCTACTGGAAAGGGTAGTGATAGTAGAATTGATAACTTTTATACTTACGGCAATGGTTCTGATACTATGGTCTGGAAAGTGCATTCTAGTACTGATAGGATTCCTTCTACTTGAAACGGGGATGTTGATCCTGCTTTTATAGCGAGAGATAATCTTGATAGTAGGAATTATAATATGTTGAATTACTCAACTACAGAGCTTATCCCGTTGTGAACCGTCGGAGTTGTTGCGTCTAATGATTATTATATTCCGATGTCCGGATTTAATCTGCATGTTCCAGATTTTTCTTTGGTTGGAACATTCAGACTTAATCCTTTTTTGTTTGGAAAGTTTGGTACTGGAACTTCGGGTTGAGGATGAGCGAAGCTTTGTACGTCTTCTTGTCCTGGTGCTCGTTTTGGGGAGGAATGGAGAGATGAAAATCAGGTTGTTGCCTCATGGACGATAAAGGGAAACTATGGAGATACTGAGTATACTCTGCTTCCATTTAATGCTCCCAATTTTAACAGGGGGGTTATTGAAGAGTGGGATACGTTGATAAGAAAAGAAAATATTAATCAAAATTCATGAACACAAAAGAATATTGTTTTTTGAGAAAATAGGCATCCTCTTAATCATGATAATAATCAAGCTCGTTTTAGTTTTAATATTATTTCTAAGAATGAAGCTCAATTGAGTGCCTTGGAAACTTTTGCTAAAATTTTAACGAATGGTAATGTAAAAAATTCTTATTTAACTTTTTCTTTGGCGAATTATCTTTGGACGCAGGGAGGACATTTAGGAAGGAATCTTTATCCTTTCTTGGAATACGAGTTTCATACACTTCATGGAGTCAGGATTTCTGATAGATTTTTTACTATTCAAGGAGAAGGGAAGGTATGAAAATATCATATCAAGCTCCAACTAAAAAAGCCAACGCTTAAACAGCCAGCTTTGGGAAGTTTTACTATTATTTTTTAGGCTTTATGAAGAGTCAACTTAAGCAGGTTTTCTTTTTGTCGATTTTTGGGTTTCTATGTACTGCGTTTGTCTTTTTTGTTCCACGAGGCTCTTCTTCTACCGAAGATGAGGGAGGGCAGGCTTGATGAGTAGTTTCACAAGAAGAAAAATCAGAATTTTCTTGAGATGTTCTGCCTCCTGTAGCTAATCTGTTTACCTGAACCTTGACTATTGCAGTTGGGAAATTTGCTGATTATCCAATATGGAAGACTTTTGCAGACTCATTTTCCAAAAAATACGGATGAAAGGTGCGTTTTTTGACAGGAGATGTTGTTTCTGGTGCCGATTTGCTTCTTTTGGCGTATGATGATTTTACTGGGCTTGCTCATCAATCTTTACGTTTTCAAGAGGATTTGAAGCCCTTTTTTATTCCACAATTGCGTAGCTTCTTTGATGAACAGTCAGATTTCCTTCCTGTTGCTGTGGATCCTGCGGTGATGGTTTGACTTTCCTGACTAGAAGAAGGATTAGATGGGTTAGAGTATTACTTTTCACAGTGGACGCCTAAAACTGCACTTTCTTCCTTTTCTTTTGGGCTCTTTAATGATCCGAACTTGAAGGATCAGAATTTGCTTTTGGCACAACAATTGGAAGATTTTATTAAGTTTAATGATGTTGGAGCTTTTAGTCAATGGCTTGAAATGAACGTTCTTTCTCCGGATCAGCAGAAAAAGCTTTGAGAACTCGCGGCTTGATCATCCCAGGTGGATCATCTTTTGCTACTTCGTAAAATTATAGGAATTGAGTTTATGTTTCATTCTTCAGTGCCTTTTTATCCGAAAGATCCGAATTCTCAGGAAAAAATTGTTTCTTATTTGTTTCCGTACCAATATGTAGGGTTGCCTGTTAGGCTTTATGGCTTTGTGATTCCTAAAGCTACGAAAAATCGTGCAATGGTAGATCAGTGGCTTCTTGAATATATGAGTACCGCTTCAGAAAAGGGTGCTCAATTCTTGATTCCACGTATGTTTCCTGTTTTTCGTGATCAGTTTAATAAACTATGTGGGGATACAGCATGTGGATTGCCTTCTGATTTTTTTATTTTGAAAGATGGAAACGCGAAAATTCAGAGATTCCTTCAGGATAAACTGTTGAAAAAGGTGATCGAAAAAAAGATTCAACCTGACTTGTATTTAAGGGAGGCTTCTGTATAATCTTTTCGTTTTTATTTTTTTTTGAAATGGATGATTGAAGAATTCGCAGCGTTTTTAGCGGAGTATAATGTCGTTGGAATGGCGATAGGTCTCCTTATTGCTGTTAAAGTCGGTGATTTTGTGAAGACAATTGTTGAGGATTTAGTGACTCCATTGTTTTTTGCTCCTTTGTTTAAGAAACTCAAGATTGATAAATTGGAGGATCTTTCTCGACATGGTGTTTTGTATGGAAAGGCACTTGCTAGATTGATCGACTTTTTGATTACAGCATTTTTGGTGTTTCTTGTGATTAAGTATTTTGGTGTTGTGAAAAAGTAAAAGGAGATTAAACCGTTCCCTTTAAGGGCTGTTGATTATTTATCTGTTTGCTTGTCTTGGTTTACTCAGATTAATTCAATGGTTTTTCTTGTAGATTCAAGAATGGTCTAAGAGAATTGGCTTTTGGTGTTTTTTTAGAGGTAGAAAGTACTTTGTAATCCAGGCTTGTTTCTCTTTGCGGTTTTGTTATGTTTTTTTGTTTTGAAGTACTTATTAGAGTGAAGTCAGATTTTTTATTTTATTAGTATGATTGTGATGGATTTTGGAAAGATGGGAGAGCTCAAGAAGATGTATGATAAGTATAAGGAGCTTCAAAAGGCACTCAAGAATTTAGTAATTAGGGCGAAAGAGGGTGAGTTTGTAGATGAAAATTGAGAAAAATGAAGTAGTATCGTTATTGATATGACGGGGGAAATGAAAATTAGGGATTTTAAAGTCAATGAAATTGCTCTTCTCGATCCTGCGAAAAAAGTCGTTTTGGAAGAATTGGTGGTAAAGGCTTTTCAAAAGGCTCAAAATAAGGCTCAAGAAGTCGCAGCAGAAAAGACAAAGGATATTCTCGGTTTTGATCCTAGCAATCTGGCTGGAATGCTTGGAGGCGGAAATTGAGGAGGAATGCCTAATATTCCAGGATTATCATAGGAACTTTTTAAGAATTAACAAAGAAAAGTTTGTTGATTTTTAATTGTTTATTTTTTTCCTATGTATAAGAGATTTTCCCTAACTGATTTAACAAAGATTCAAAATAATTTCATTCCACATTTCTATGCGATCATTAAGAGAGAGAAATGTCAGTGAGCGAATGATTTTTTCAAGATGTGCTTTCGTGAAGGGAATGATCTTTATTATTTTTATCGTTCAGATGTGGTTTTTAGGATCTCTAAGCATGAGAATCAGCTTTCTTTGATTGATGAAGAGGATAAAATTGAGGTAGTTTTGCCTTTTGATCAGAAATGAGGTCTCAACTCTCTTGTTATGAAATATGTCAGCAAAAAGGAAAGACAAATCTGACAGAAAACGATTGAACAATTATTGATGGATGAATTTAAAACTGGGAAATATTCTAAGATTCTTGATAAGCCTTATTTGGTTTACGATATTGAAACAACTACGATAGATGATCTAAGATCTGCAAAGTTTTTGCTTGCTTATGCTATGTTTCCTAATGGCGAAAAAATGGAATATCGTTGTATTATGCAAGATGACTTGAAGGCATTTGTAGATGAAATGCTGGCATTTGATGGCTATATCGTTGGGTTTAATCAGATTTGGTTTGATAATCCTGTGTCTGCACGGAATGTAGGGTATGGGGATCGTGAAATTGAGATCCTCAACCAAAAAAGTATTGATCTTTATGTCTTTTTTCAAACTCTTACGAAAAGGAGAATCTGACTTAATAAACTTTCAGAAGCTCTTGTTGGAGTTCAAAAGACGCTTTCCTCGGGGGCAGAGTGAGAGGTTCTTTGGAGGCAGTATCAGGATACAGGAGATGAGAAGTATTTGGATGAATTTAAAAAATACTGTAAGAACGATGTGAGGATGACAGCTTTGGTAATGTTTTATTTTTTGCATTACAAGAAAATTTTTATGGATGGGGAAGAATATCCTTTTGATATTGAGGAATTTGTAGCAAAATCTCGTCATCATTCTCATGAAGAACAAGAAACTCCTGTTTTTTGAGGTCAGAATCAGTCTATTTTTTAGTTTTTATGATTGAGCGATGAAATGTCCAAAGTGTAAAAATATGGATTCAAAAGTTGTGGATTCGAGGATGATTGAAGAAGGGACAATTATTAGGAGGAGAAGGGAATGTGAGAGGTGTGCCTATAGATTTACTACTTTTGAGAGGATAGGGTTGACCGAGCTCGTCGTGATCAAGAGAGATGGAACTAAAGAACTCTATGATAGAGCAAAACTTAGGAAGGCTGTTATGTTGGCTTTCGCTAAAAGACCGCTTAATGCCGAGGTTATTGATTTGATGATCAATAATTTGGAAATTTCTTGGCAGAATGCTGGATCAGAGGTTTCTAGCCAAAAAATAGGAGATGATATTTTGTCTGCGCTGAAGGAGATTGATCCTGTGGTTTACGTAAGGTTTGCTTCGGTTTATCAGTCTTTTGAAAATTTTTCCGACTTTAAGCAGTTTATTGAATAAATGAAATTATTTTTCAGAAAATCGCCTTCTGATGACAAATTTTTTGCTCCTAGGAAGCTGGGAAGTTTCGTAATTATTAACTGGGGATCTGGTCTTAAAAAGCTCTGTAAGTCTGGCTTCAATTGGGACGATTTCGATTCCTCATGGAGCTTTTCCTCTACCTCTGCTTTCAATTGTTCTTTTCTCGTCCAGCACGATGACTACGTGTCCGTCTCGTACTATTAAATCGAGAGGTTTGAGTAGAGGAATGATTTCCTGAATTTTTTTCCCTTCGATATTTATCGGTTCTCAAAAGTTCAAAAGGCTACGAGAATTTCTTGGTGTGAAGCCGTTTGTCGCTTGGTAGAGAAGGCCAGAGCAGTCTACTCCTTTCATGAGTTTTCTTTTGGTTTCTAGGGCTGAGAGTTTTGTGGCTGAAGGAAAGTATTTTTCAAGTTCTGGTATTCCTTCATATCGAGAGCCGCCCCATACGTATACAGAACCTGATGTGGCTAGCAATTTGTTTGTTATGTCCTCTAGTGAGGGGAGTGTTTGTGCTTTTTCTTCAGGTTTTTCTGCTGTTTTTTCGATGAATCTTGCATCAAGAAAATATCCAAATTTTGCACCCCTTCCTGCATCAAATTCTCTCGTGCGGATTTGATAATAGACGAAATCTCCCTTTTGAATCTTCTTCATTAGGCTGACTACCGTGTCTGGAGGGAGGACGACAGCTAGTTCCTCGATCATATTGTATTCATTTACCTTCATACCAGTTGTGCCTTTTCCTCAAAGTATGTTATTGAGATCTACATCATCAATTGGGAGTCGGAAAACTGGTGTAGGAAGTTTTGTTTTAGCATAACCTTCGAAAGTATCTCGGCTTTCGATTTGTAAAGATCTTGATATTTCTTCTGCGAGTTGCTCTGTTTTCAAGAGCTCTTCTAGGATTTCTTTTTTCTTCTCTTCTGGTAAATTTGCGATAGTAGCGATCCTGCTCTTGATGTCATTTTGGTCTTTGGCAGAAAGCTTTGCTCAATAGAGCTTTGTTTTTGTAGGAGAGAAGGCCTTGCGAATGGCGAGAGATTTTTGGGCTAAACGAGAAAAGAAATCAGATTTTTCTTTCTTTTCAGCTTTAGAAAGATTTTGGTTGAGTTGGCGATCAATTTGTAGGTGGTCTGCTGTAGTAAACGGTGTATATGCCCAGCCATTTCCTGTCATCGTTAGGAACGCTACCGTAAAGAGGATAAGTTTTTTCATGTGAATTTCTGTGCTTGAGAATAAATGTTAGGAGTATTTTCTTCCTTTGAGTTGTGGAGGAAAGTGTTCTTGCTGTACTTTTGCATCGGGAAAGTCGTGTGCATACTGATATCCCTTACCATAGCCGAGGGTTTTCATTGTCTTGGTAGGGGCGTTTCTTAGGTGGAGAGGAACGGGCAGATTACCATAGCGTTCACAATCGGATTTTGTTTGGAGTGAAATTTTGTAAGCGAGATTGGATTTCGGAGCTTTTGCAAGGTAGATGGCGAGCTGCATTAGGAAAACATCGCATTCAGGCATTCAGATTTTTTGTACTGCGTCATAGACTTGGTTTGCAAGGAGTAAGGCGTTATTGTCTGCTGGACCTATGTCTTCACTAGCAAAGCGGAGTAATCTTCTGGCGATATAGAGCGGATCCTCTCCTGCTGTTAACATTCTTTGTATTCGATAGCAGGCAGCATGATAATCTGAGTCACGGAGGGATTTGTGGACTACAGAAATGATATTGTAGTGCTCCTCTCCGTCACGGTCATAGTAGAGCTGCTGTGTTCCAGCGTTTATTAAGATTTCTTTGGTAAGCTCTCCCTTCCCCAGTAGTAATAACGCGGATTCTAGCAGATTGATCGCGTTTCTAAGATCTCAATTCGCGGAAGACGCTAGGGTTTTTAGGCTTTCAGAAGGGATTTGGATTTCTGGGTATAGAGATCTGATTTTTCCTTTTTGTAAAGTAAAAAACCTGATCATTTCTTCGGGACTGATTTTCTCGAAAATGAAGAGGCGACAACGACTAAGAAGTGCGTTATTTACCGTAAAACTCGGGTTTTCTGTCGTGGCTCCTATGAGCGTGATAGTCCCTTTTTCTACAAAAGGTAAAAGGGCATCTTGCTGTGATTTATTTCGGCGATGGATTTCATCGAGGAAAACTAGTGTTGCCTTGCCGTATTTTTGGTTTAGTTCTGCTTGTTGGATGATCGTGGCCAGGTCTTCTTTTTTGCTCCTCACTCCAGAAAGGGGAAAAAAGTCTGCCTGTATCTGAGAGGCGATAATTTCTGCGAGAGTCGTTTTTCCGCAGCCTGGAGGTCATCGAAAAAGTAAAGAGGGAAATTTTTTTTGTTCAAGAAAAACGGAAAGTGGCTTTCCTTCTCAGATAAGGTGCGGTTGTCCAACGAAAGTTTCAAGTGTTTTGGGGCGAAGGAGAGAAGATAACGGTTCCATGATTTATGAGGAATAGAGAGAAGGCTAAAGAACTGAGTTTTTTGTTTTTGTCGAATGTAATTGTTGTTTTGAAATCTTAAGCAGTAATATTGTCGTTCGTTTATAGTAGCTATTATCTTTTGAAGGTCAAGCAGATTCCTTGGAAAATGAGTGGTCTTTTGTCTGCTTTGTTGTGTTAGTTTTTATAAAAGATTTGTATTTTTAGAAAGTTTTTATATACTTAGTTTTGATGTATATTTTATCTTTTTTGTTATAAAGATGTTGTATGAGAAGCTCTTGGATGATTATAAATGTGCTATGAAGGAGAAGCAAGAAGTTAAAAAGTCTGCTTTAAATTTTGTTTTAGCGCAGATTAAGAATAAAAAAATTGAACTTCAGAGAGAACCTAATGATGATGAGATTATTGCTGTTCTGAAAAAAGAGGTAAAAGCCCTGAATGAGACGATTTCTTTTCTAGAGAAGGCACAAAAATCTGAAGAGTTAGCTGAAGAACAAGAGAAAAAACATGTTTTGGAATCCTATTTGCCTCAGTTGTTGGATAAGCCAACGACAAAGAAACTTATTGAAGAGGTCCTTGCACAACTTGGAATTTCTGATCTTAAGACAGGAAGAGGACTTTTGATGAAAGAATTGATGGCTAAGCACAAGAGTGAACTTGATGGGACTCTTGTAAATGAGCTTATCAATGAGATGCTTTAGTTTTTCCGTGGATATGATGAGAGTAATTAAGTCTCTGATTTGTGCTTTTTTGATGATGATGGTCAGTTTTTGAGCTATTTTTGCTAATGGGAAGACTTGTAGTGGAATAAAACTTAATACTGATGTGCCTTTTGTGGGAAATTGTATTGAAACTACCAAGGGAGGTGATAAGACGAATCAGATTAACGCTTTTCCAAAGTTGATGTGAGCGATGATGAAACTTTTGATGACGGTGATCCTTGTAATGTCGCTCTTGATGATCGTTGCGGCGGGAGTTATGATGACGACAGCGTGACTTGAATCTAGAAACTATACTAAGGGAATGGATATTATTGAAAAAGTTGCTGTTGGAATGGCGCTTCTTGGCGCTTCTGGTGTGATCCTTAAGTTGATAAATCCGAACTTTTTTGGCTAGGAGCGCTTGAAAACGAGGCGAAACTTTGTAGACTGGATCAGTTTTTATCTTTTTTATTGGAGTAGAATGACTGTGCAGATTAGAATTGATGGAGGGTTTCAGATAGAAAAATCTTTATTTTTTGGATATGCTTATGCGCATAACGGTCTCATCAACTTGGCTTCTGAAATGGGTGCAGATATGAGGTATAATGAGGGTGAAATCTGTATTGTAGATTATCCAGGAGAGTATGATATTAGAGGATGGACGATTAAGGCTTTTGTCGGGCAGAACGCTAAGCTTAATTATTTAATCCAAGGAAATGGGAAAAAATTCTGAATTATTCAGTCTTCAGATGTCCTTGAACTCGAGGAAGTTGATGGAATGGATACATGGCTTTACCTTGGAGAATCTATTGAAAAGAAACTTGATCAGCTAGAGCTAGAAGGGGAAAGAATTAATCTTATGGAGTTTAGTGAAGAGAAGTAGTCTTCGGAGGAAAGTTGGAGTTTTTTTATGCTTTGAAGTTATTGATTGCGTCTTGAAAGAGTCTTCCTTTTTCTTCGAAATTTTTTCGGATGCTGTAGCTTGGTGAAGCAGTAGAGAGGAGGCAGATTTGGCCTGGTTCGGTATATTGGAAAGCAAAATCTACTGCGGCTTGCATCTGGTCAGTATGTAGGATTTTCCCTTTGAAGTTTGGGATGAGTTTTGCCATTTTTTCTCCAGAAGGTGGGAAAAAAACTAGGTTATGGATTCAGTATTCTTGTACTTTTTGCATCAAAAGCTTAAAATCATAGCCTCTGTCGGTTCCTCCGAGAAAAAGGGTTCCGACTTTTTTTCAAAATGTTTTGAGTGCTTCTAGGGTGCTTTCTGGGGTGGTGGAAATTGCGTCGTCATAAAAGCTAATCCCTTGAACCTCGCTTACGAACTGTAATCTATGTGGTAGTCCTGTAAATGATTTTATGGTATCGTGGATTATATTTATAGGAACTCCGATTTTGAGGCAAACTGCGATGGCAGCTGAAATATTCTGGATATTGTGGTTTCCCAAGAGTAATTTATCAGCAGTAGGGAAAAGTTCTTGTGTATTATGCAGGAAGTATCCGTGATCTCGTGAAGTTTTCCCTCAGATTCCATAGGTTTCGATGTTTGTGTAGCTTTTTGTAAGTTCATATTCTTCTGCAGTTTTTTGCAAAACAATATTAAAATCTGATCCTTTGAGAATATTCAGTTTTGCCTGTGCATATTTCTCAAATCCTCCGTGCCGATCCATATGTTCTGGGAAGATATTTCCAAGTACTGAGATATAGTTTTTCTTTTCTAAACGTTCCAACATATAGCTTGAAAGCTCGCAAACTACAAAATCCAATTCTTCATCAAAATTAATTTCCTCTAAAACGGGATTTCCGATATTTCCAACTAACTTGACTTTGTATCCGGCATTTTTGAGGATTGAGAAAATAAGTGAAGTCATGGTAGATTTTCATTTACTTGCAGTGACTGTGATAACTTTCCCTTGGTACGTATTGAAAAAAAATTGCATCTGAGTCAGAATTTTTTCTTTGTAAGGCAAGAGTTCGGAGCTATACGGCACTCCAGCAGACTTGAAAATTACATCAAACTGGTCAAGATGGTCTAAATAGTGTTCTCAGGTCTCATTTGAAATGTCGAGCTCTTGTAAAGCGGGTTGATCTTTCATATCTAGGACAGAAAGTTTATTGAATGCGAAATTATTGCTGAGCAGGAAGTTGAGTGTTGATTTTCCCTCCAGTCAGAAGCCGAGAATAGCGATATTTTTGCCTTCAAATTGCTTGGTGTCCATCATCATATAGGAAAGAGGCTAAAGTTGAGAAACTTTGTGGTCGGCTACTTTTTCTTGCTTGTGGTTTTTTTCTTGGCTGGAGCTTTTTTGGGAGCGGATTTCGTAGAATCTTTCTTTACTCCGATGAGCTCCAAACAATCGTCTAACGTCAAATCACTCGCGTCTTTTCAGCCTTTTGGAATTTTATAATTTTTCTTGGCATATTTGATATAAGGACCATAAGGACCGTTCAAAACCTGAATTTTCTCCTTTTCGTAGTCGAATTCATTGATGTATTTATTTTTCTCAGCCTCCATTTTCTCCTGAATCATCGGAATGGCTTCTTCTAGCGTGATAGAAAAAGGATCTAACCCAGAATCTTTTTTGATCGATACAAAAAGGCTTTTGTATTTTATATAAGGACCGAAACGTCCTGTAGATGCGGAAACTTCCTCTCCTTCTCGTTTTCCCAAGCTTCTTGGTAGAGCAAAAGCCTGTAGCGCTTCTTCAAGGGTAATCGTTTCAATCGTTTTCCCCGTCGGGATCGAAGCAAATTTTTTATCAGGATCGTCTTGGTCTCCAATCTGAATCATCGGTCCATATCTTCCCATTCTCGCGATAATTTGTTTTCCATTGCTTGGGTGCTGACCGAGGAGTCTTTCAGTATTTACTCTGTCTTTTCCTGTGGTTTCAACGACATGCTCGTGAAAAGGTCCATAAAATTTTTCCATCATTTGTGTCCATTTTAGTTTTCATTCTGCGATTTCGTCAAACTCTTGTTCCACATTTGCGGTGAAACTATAGTCCATAATATCAGCAAAATTCTGATTCAAAAAGTCAGTTACGATCATACCAATATCAGTCGGAAACATTTTTTGTTTTTCGGCTCAGGTTATTTGATCTCTTGTTTCTCTTTTGATTTGTCCTTCGGTATAGGAGAGCTCTACATAGCTTCTCTTGGTCCCTTGACGGTCTTCTTTGACAACGTAACCTCTCTTGAGAATAGTTGAAATTGTTGGAGCATAGGTTGATGGTCTTCAGATGCCTTGTTCTTCTAGCTTTTTAACGAGACTTGCTTCGGTATATCTTGGTGGATGTGGTTTAAACTTTTCTGTTGCGAGGATTCCTTTTTCTTGGAGCATTTCTCCTTTGCTGAGCTTAGGGAGCATTCCTTTTGTTTCTTCATCATCCTCTTCATTATCTGTGGATTCCATATATACTTTTAGAAATCAGTCAAACTTGAGAACTTCTCCGCTCGCTACAAAATGAAACTTATCAGTTGTAGAAATCTGAATTGTTGCCTTGGTTTTTTCAAGCTCAGCGGGAGCCATCTGGGAAGCGATCGTTCTTTTCCAAATCAGCTGATAGAGCTTCTTTTCGCTCGGATCTGCCCCTGCAATGTGGTTTTCCATATGTGTAGGTCTGATACATTCATGGGCTTCTTGTGCTCCTTTTGCCTTGGTCGCATAATGCGTTGGTTTGGAGTAGTTTTCTCCATATTCGCTGACGATCTGGGCTTTTGCTGCCTGTATCGCAAAATTAGAGAGATTCACAGCATCGGTTCTCATGTAGGTAATATGCCCTGCTTCATAGAGCTTCTGAGCGACTTGCATCGTTCTTGCAACGGAAAAACCAAGTTTCCTACTTGCCTCCTGCTGGAGCGTTGAGGTGGTAAAAGGAGCTGAAGGAGATTTTTTTCAAGGTTTTACCTCGATATCTTTGATTTCGTAGTTGGCACCTTGTAAAAGTTCGAGGAATTTCATCGTTTCAGCTTCAGATTTCAACTGTTTGTTGAGTTCTGCGGTAAAGGTTTTTTCTTCTTGGCTTAGAAATGTTCCTATGATTTTGAAAAAAGAATTAGCTTCAAAAGCCTGAATTTCTCTTTCTCTCTCAACGATCAATTTTACTGCTACGGACTGTACTCTTCATGCAGAGAGTCAGGTTTTGATTTTTCTCCAAAGAATCGGAGAAAGTTCAAATCCCACTAGTCTATCCAAAACTCTTCTGGCTTGCTGTGCATCTACTAGGCTCATATCAATGGTTCTCGGGTTCTTGACTGCGTTTTCTATTGCTGTTTTTGTGATTTCGTGAAAAACGATTCTTGGAGTCGTTTTTATGTCCAATTTTAGCTGGTTGGCAAGGTGTCGTCCGATCGCTTCTCCTTCACGATCCTCATCGGTTGCGATCCAGGTTTTTTCTGCTGACTCAGAGAGTCTTTTTAGCTCTGAAAGTACCTTCTTTTTGTCCGGAGAGACGACGTATTTGGGAGTAAAGCCATTGGCAATATCGATTCAAAGTTCTTTGGTCGGGAGATCTACAGTATGTCCGTATGAAGCTCTCACTTCATAGTCAGGACCGAGAAACTTTTTTATGGTCTGTGATTTTGCAGGAGACTCAACGATTACGAGGTTTTTTGCCATTTTGGCGTAGGAAGATAAAAGTTTTCGTCTCTTGGTATAGGGAAATCAACGGGAAGTTCAAGGAAAAATTGGAGGGTGAGATTTGCTGTGCTGAGAATTGTTGGGTTTCTATGTTTGTTTTGCCCGGTTTTCTTTTAGGATTACTGTGTAAATCTTTCCTTTTCCATTGATATTGGTGATAGAAGCCTCAGTTTCCGCATCTAAGAGTAGAGTATCACCTTTTCTAAAGGTTTTATTTTTAAAGTATTGGGTAGTTGCGAGGTATTCTCATTCAAGTGGGTAAAGGAGGGATTTCCCTTTATGACTTACAATATGTTCAGATTTTCCCTCATCAAAAACATAAACACGGATGTGATATGGCATTTCTAAATCATTCAAATCATGTCCAAAAACATGGTCAGCTTTTTGATCTAATGTTCTTTTTTCTCCCTGTCCTACACTGCCTTGATAAACCTTTCCCCTATCCAATAATGCAGAAGGAAGCTTAACGGATAAATTCATAACAGGATAATCGGTAGGATTTTTGATCGTATGCCAAGTTTTTGGAGGGAAAATGATGATATCTCAAAAATCAGCCTGGATTTCATGTTCAATTCCTTCAGTTTTGAATTTGCTTATTGAGGATCAAGTATAAAAGGTAATCTCTCGGTTTTCTCAGTGATCGTGCCATGGCTGTTGATAGTTTGGCGGCGTTTCTGCTGTTACTAAGGAAGGAGCATCTAAATCTGAAGCTACTTCAGGAACGATTCCAATATATTCTCTCGGTTCCAGAATATCTCTCCCTAGAACTTTAGAGGTATTGAGATACATTTCTTTTTTTTGATATGCTGAAGCGATAATTCCTGTTGTTGCTGGAAGGATTTGTTGGCTAAAATTTTCAAAGAAAATCAAATTCAAATTTTTTTTGATATCTTTATATGTATCTTCAGAAAGAAGTTTTTGCTCATAATAGAGTTCTGTAAGAAATTTTGCGACTTGGGCTTTTTGTCTTCTAGAGAAGAAATAAAGTGGCTTATTGAGAGTTTCTCTGTGTGCAAGAGTATGGAGTTCTGTTATGAGTTGGTAAAGGTCTTTGAGTTTTGGATTGCTATTCATGACTTTACCTTTTACGAGTAAAAATTACCTATCTTTAATGGAGAAATGAATTAGCGTTTCTGATGAAAATAGGATTGAATTCGATCAAAAAGCTCATCAAGATCAATTTCAAGTTCACTGTCAGAAACGGGGAAACCTTGTTGCAGGATCAGCTTTTTTGTCTCTTGAGAAGATACCTTTGCAAGATATTTGTCAGGAGATTTCAAGCTAAGGTAAATAGCCTTTAATCAGAGGGGATTCTTTTGGATTTGAATATCAGTAATATCTTTCCATAAAATCTTCCCGATTTGGCGTCCTAAAAGTGTTTGTTGAAATAAAATTCCCTCCTGATCTAGATATAGACCTCTTTTATTTGGGGGATTGAAGGCAAAATCTGCGATATTATAGACAAGCAAAAATAAGGAAGCTCCTGAGAGAATCCAAAGTGAAATAGTTATCCTGGAAAACTCGGCATAAAGCCATCGTGCTATTCCTCATAATAGCAGTGTCAGAGCTCCCAAGATCAAAGATAGTTGTCGCTGTTTTTTTCTACTTGGTGCAATAAATTTAGTCGTCATTATACTAAAAAACATCTAAATACAGACCTCATGAAGAAGAAAATCTAAGCAAATAACGTTAGAAGATTTCTACACATCTAAAATCCTCTCTTTTACCCTTTCTTTTCCCATTACTTTCATCACAGAAAAGAGGTCTGGAGTCTGTTTTGCTCAGCAGAGCTGAATTCTGAGAAACATTGCAAGATCTCCAACTTTCCCGACAAATCCTCACTCCTTAAATTCCGCATTATTCCCTGCGAAACCATATTTCTTTCCAATCAGTTTAAGCTGATCAAATCGGTCAGTTACCTCGCCATCTAAGCTGAAGTTTTCCGCATAATCGGCAGCAAAGGCTTTCCAAGTCTCAAGAGGAATCTGCTCTGGGAAGTCAGGTTTTGATTTTTTGATTTCAGTTCGCTTCTCATCGTAGAAAAAGAGGATATTTTTCTCAATATCGCTGTAAAGCGTAAATCTCTTCGGGTCTTTTTCCGTGTGCCTTTCAATATTCAGTGCAGCTTTGGTGTATTCGGCGTGCTTTTCCATCAGTTCGGCAAGCTCCTTGTGATAGTCCTTTGCCCAAGCGAGTCCTTGTTCATAGAGTTCGGCAGTGTTGAGCTTGGAAAGGTAGCCGTTGTTGATATTCTGGAGCTTCACAAAGTCAAAAAGCGCTCCTGAGACATTCATCTTCTCAAGTGAGAACTGAAAATCCAAAAAAGTCTGATTCTCATGCGCTCTTTGCCAGTCTTCATAGCTAGAATCTGCGAGCGTCATGATGTATTCCAAAAGGGCTTGTGGAGCATATCCTGCCTCAAAAAAGAATTCTACATTTGCCTCTGGGTCCTTTCTCTTGCTCAGCTTTCTTTTCTTTCCTTCATCAAGCTTACATATCGGAGCCAAGTGGCAGTATTCAGGTGCAGGCAAGTTAAAGGCTCCGAAAAGCTGGAGATGAAGCGGAACGGAAGTCAGCCACTCCTCACCTCTCGTGATGGTCGTCACTCTCATCAAGGTATCGTCCACAATATGTGCAAGATGATAGGTTGGAAGCCCGTCTCCTTTGATTAGCACAATATCATTATAGTTGTCAATCATAGAAATCTCGCCTCTGATCAGGTCTTGGAAAACGATCTTCTTGGTCGTATCGCCATGAGAACGGAACCTCAAAACAGGAAAAGTCTGACCTTCTGCATTGAATTTTTCTAGGAGTTCGTCTGGAGTTTTATTTCTCCATTCGGAGTAGTTTCCGTAGATTCCGGGGATTTGCTTGCTTGCGGTCTGCATTTCTCTGATTTCATTGAGTCTTTCTTCGCTCATTCGGCACGGATAGGCGAGTCCTTGAGCTACTAAATGCTTTGCAAACACCTCATAAATCGACCTCCTCTGAGATTGCACATAAGGACCGTAGTTTCCAAATTCTTCTGTCAAAGTAAGGAGTCCTTCATCAAACTGAATTCAGAATTTTTTGAGGATTTTGAGTAAAACCTGGGTGGCTCCTGCTACTTCTCTTTTTTGATCGGTATCTTCGATTCTGAGGAAGAAAACGCCGTTATTTTGTCTTGCGTGTTTTCGCCCAATAAATGAGGTATAGAGATGCCCAAAATGCAAATATCAGGTTGGACTTGGTGCGAAACGAAGCACTTTCCCCTCAGGTCTTGGAGGGTATTTTGCCAAAAGGTCATCTATGCTTTCTTTCACTTGAGGAAAGAGGAGATCTGCGAGTTCTGCTCGGGTTGTCATGGTGTTATGGAATGAAGATATAAAAATCTGTATTTACATACTTTCAGGTGTCGAGATTCCGAGGAGCTCAAGAGCATTTTTCATCACACACTGAATCGCTTCAATCAAGGCTAAACGAGCCGTGCTCAAAGCAAGATTGTTCTCGTCTCGGATTTTCGCTTCGTGATAGTAGCTTGAAAACTGCTTTGCCAGGCTTAATGCAAATCTTGCAATACCATTAGGTTTATATTCGCTGGCTGATTTCTGCACGAGGTCTGGGAAACTCGCAAGCTGAAGGAGCAGATTTTTTTCGCTTTCTAAAGTCAAAAGGCTGAAATCTGGCTCTGAAAGCTGGGAACGATCCACTTTTTTGAAAATAGATGAAATTCTCGCAGACATATACTGTAGGTAAGGACCAGTCTCTCATTCAAAAGAAAGTGCCGTCTGCTTATCAAAAGTGATCCCTTTTTCTGAGTCTTGAAGGAGCATCCCGAATTTCATCGCAGCAAAAGCTACATCGTGAGCAATTTCAGCTTTTTTTTCTGCTGGTAAATCACGATCAGCGACTAGCTCTTCCGCTTTTGCAAGCAGTTCGTCACGGAAATCCTCATACAAAACGACATTTCCTGCACGCGAGGACATTTTTCCATCAACTAAATCCACCAAACCATAGGATACATGTTTCAATTTATCGTGATCAAATCCAATGAGTTCCAAAGTTTTGAAGAGCTGCTGGAAATGATGCTCTTGCTCTAGTCAAACTACATATAGCGACTGATCATAGTCAGGGAAATCAGACTTTTTCTGGTAGGCAAGCGCAATATCTTTGGTAGAATAAAGCGAAGCTCCCGTTGATTTGAGAAGAAGAAAGACCCCAAGCCCAAATTCTTCCAAATCCATCACCGTTGCTCCCTGTGATTTCTTTGCAATGCCTTTCGCCAAAAGCTCTTCAACAATGCGGATTCCTGGCTGCTCAACGTCACTTTCGTAGTATCGTTTGTCCATCTCTGCACTTCCAAGCTCAGCAAAAATATGCTGTATATCTTGTAAACAAAGCGCTCTTGTTTCCTTCCAAAGCGCAATCAATTCTGAATCTCAATCCTCAAGCGCCTTTTGGAGAGCTTCAATCTGAGGCTTGTAAAGGTTAGGATTCTCATCTACTTTTCTCGTCGCCAAAGTATAGATCTGTCCCACCCATTTCGTAAAATTCTGCTCTGGGAAAGGCTGCTGATCAAAGTTTTTGTAATACCAAATCCATTTCGCTACATGTGCCCCAATATCGCCTGGATAGCAGGTTTTGATGACCTTATTTCCAGCGAATTTTAAAACTCTAGCAATACTCTCACTTAGAAGAAAATTTCTCACATGCCCGATATGAATGGATTTATGGGTATTTGGAGCCCAACCTTCAAGAAGGATTGTTTTCCCAGTCTTTTCTCCTGCTCCAAAGTCAGCTTTTTCTTTCTTGATCTGCTGAAGAACTTCTGAGGCGAGAACTTGAGTATTGAAGCTTGCATTTACATAAGGTCCTACCGCATAAAATTCAGAAAAAAGAGAAGAAGTCTGATTTTTCTCCTGAAGAAAGGCAAGCACTTCACTCGCAATCATATTTGGAGCCTTCTCCAGCGACTTTGCAAACTGAAAACACGGGAAAGCTAAGTCTCCTTTAACATTATCAGGAGCCATCTGAATAAGAGGCAAGAGTTCCTGCTTTGAAACTTGGAAAAATGGAGAGAGGAGTTCGGCAAATTTATTTTTGTAGGAAAGGAGCATCAATCAAAAAGCTAACATAAAACGCTATGAAGTATAGTAATTCACAAGCAGATTGCAAAGTTTTTTGTCTGATTTTTTTGCTGGTATTTGCTAAAATTAAGAGAACTCTTGATTTCTGGGACTGATTTTTTATTATCAGCATCGGCTTTTGTTTTCATTGGTGATGGAAGTTTTTGGGGTAGATACGAGATGAAAGCCTTTATTGTTAAAGCTTAATCCTTGCTCCCTTGTGCTTGGAAAAGTCCATAGTATGCGAAATTATTAGGAGGGGAGAAATGGCTTTTACATCCTAAGATTGTTCTATATTCTCTATGTGTATATGCGTTATCAAATCTTAAATCAAGATGCAGATTTATCTTTAATCCAAAGACTCCTAAAAGTAAGAAATGTCTCAGATGCTCCTCAAGATTTCCTCAATCCAAGTATTGCAAATTATCGGTTAGATCCTTTTTTGCTCAATGATATGGAAAAATGAGTCGATAGAATTATCGATGCGATGAAGCGTAAGGAAAAAATTATGATTTTTGGAGATTATGATGTAGATGGGATTACTTCGAGTTTTTCGGTTTATAAATTTATTAGCTATTTTTTGGGATATAAAAATATTAGCATTAGATATCCCAACAGAGTTGAGGATGGATATGGGCTCAAAAATAAGCATTTGGATGCGATGAAAAATGAAGGAGTGCAGCTGATTATCACGGTCGATAATGGAATCGCATCCGTGGAAGAGGCAAAGTATGCTAAAGAGCTTTGAATAGATATGGTGGTTACTGATCATCATCATGCTCTTGAGACGCTTCCAGATGTTTGTGCATTGATTAATCCACAGGTTTCTCCTCTCTATCCTTTTAAGTGATTGGCTGGAGTTGGGGTGGCTTTTAAGCTGATTAATGCTCTTCTCTCAAAATCTACTTTTTCTAAAGAGAAAAAAAATCAGATTTTTAACTATTTTTTGCCGATTGTGGCTATTGGGACGGTGGCTGATGTGGTTCCTTTGGTCCAGGAGAATAGGGTTATTGTCAAAAGAGGCTTGGAACTTATGAATTATCATCCTGATCTTTTGCCCAAGTGATTGCAAGGTTTCCTTAATTTTCTGAATCTTAAGGGGAAAATTGATACGTTTCATATTGGGTTTGTGATTGGTCCTAGGATTAATGCCTGAGGAAGAATTGAAAGTCCCTACGATAGTCTTAGGATTTTCCTCTCGGAGTGAGAACATCAATTGCCGTATCTAGAAAGGATTGAATCTATCAATACTGAGAGAAGGAGAATGCAGGATCAGGCTTTTAGAGTGGCAGAAAAACAGGTCAATCCTGATCAGAATTTTCTTTTTGTTTGTGATGAGTCGTTTCATGAGGGGATTGTAGGAATTGTTTCTGGGAGAATTACGGAGAAATATTACAAGCCTTCTGCTGTTTTTAAGCTTGATTTGGAGAAAAAACAGGCTGTTGCTTCCCTTAGAGGACCTGATTATTTCAATGTGATTGAGATGATTATTCAGGCTTCTCCCTATCTGAAAAGATTCTGAGGACATAAGGGGGCCTGAGGATTGACTGTTGATCTGGAACATCTGGATACGGTGCTTGGTATTTTCCAAAACTATTGTCAGTCTCGTATTACTTCAGAACAACTGGAAAAGGTTATTCAGGTGGATACGTGTCTTCTCTCTCATGAATGGAATACAGAAGAACTTTCAGAGATTGAGCAGCTTGCTCCTTTTGGTGAGGGGAATCAAGAACCTAATTTTCTACTAGAAGGTATTAAGGTTGAAAAGGTAGAGAAGGTTTGAAAAAATGGTGGGTCGCATCTTAAGATTTATGGAAAATTCGGTGAACAACCGATTTCTAGCCTGTTTTCTAGTAAAGGAGTAGAGATTGATAGTATATGAAGTCAGGTTTCCATTGTTGGTGCGGTGAAGAGAGATTCCTATAATTGAGGGTTTTATGTCTATGGTAATAGTCTGATTTAGTGCTATTTCTTGTGATCTATAAAAAAAGAAGGGGCAAAACTCCTTCTTTTTTCAGTTTTATTTTTTTAGTCGTTATAGCCCTGTTCTTCGAGTGTCATTCCGATAATTTCTTCATTGTTATCTACTGGAATGTTTTGCACGGCTTCACGATTGATGTATACATACATGAGCATGAGCATTGGCACTCTACCGATAATTTTGGTGATGTATTCTCCAAGTGCTTCTCTAATGAACTTGAGATTATCCTTGATATCCATTCTCTTTTTGCAATTATCGTTGTATTTTGCTCTTGCGAACTCTACAATCTGGGTATGAATCTTTTTTACCTCTGAAGAATAGACAAATCCTCTCGATTCAATTTGGATATTTCCGACGAGTTCTTTGGTTTTGGTATCAACTTTGAAAATCAGACCGACGATTCCATTTTCTGCCATAATAGATCTGGCTTTCATTACATATTCTCCAGAAAGGTGCCCTTGTCCTTTCCCATCAATCATTACGGTATCAAGCTTAATCCTCTTTTCTGAAACAAAAGCTACTTCGTCGTAGAGCTCTACGATTTGTCCATTATCAGGAAGAAGTACCTTATCTTCAGGGATCCCCATCATCATAGCAACTTTTTTGTTTGCATGTCTCATATAAGGTTCTCCGTGGATTGGGCAAAAATATTCAGGATTCAAGAGAGAAAACATCATCTTCAAATCCTCTTGGTATCCATGTCCTGAAGTATGAGTATCTAAACCAGGTTCATCAATGACTTCTACGCCTAGACGAACAAGGTCGTTGATCATTCAGATTACTGCTTTTTCATTTCCAGGAATGGTGTGAGAAGAAAGCAAAACTGCATCTCCTGGTCTGAGCATGAAATCTTTCACTTCTCCTTTTGCCATTCTTACTAGAGCTGAGTTTTCTTCTCCTTGAGAACCGGTGCAAAGCACAAGTACTTTTTCATCAGGCATAGACTCTGCTTCTGATGAGAGCTGGCGAATCATCCCCTTAGGAACATTGATATATCCGAGTTCTTGACAGAGCTGAACATTAAGTACCATAGATCTTCAGGCGAGGAAGATTACTTTATCGTATTTTACTGCACTTTGGATGATCTGGATGATTCTTCCTACGTTAGAAGCGAACGTAGAGACGATCATTCTTTTCTTTTTGTAGGCGTTAAGGATTTTATCGATATTTTCTCCAATTACCTTTTCTGAAACGGAATGCCCTGGTTTATTTGCATTGGTAGATTCTCAGAGGTAAAGTCTGACTCCTTCTTGACCGATTCTGGAAATTTTTCCGAGGTCGGCAGGCTTATCTATCGCTGGTGTGAAATCAATTTTAAAATCTCCAGAGAATACAATTAATCCTTTTGGAGTATGAAGGGAAAGTCCCATAGATTCTGGTATTGAGTGATTTACTCTGAAAAATTCTAAAGTGAAGGCTCCAAGTTTGAAAATATCCATATCAGGATCAACGAGCTTGAATTTCAGATTTTTTGCATCTTTTTCATCCAAACTCCTCTTGATCATACCGATTGTTAGAGGAGATGTGTAAATTGTTGGATACCCTAGGCTTTCAATGATGTTTTTTACTGCTCCAATATGATCCAAGTGTCCGTGTGTGATTACGATTCCTTTGATATTTTTCTTTTTGGCTACTAGATAGCTAACATCAGGAATAATGTAGTCTACACCTGGCATTGTGTCGTCGGCAAACTCCATCCCAGCATCAATTACCAAAATATCATTCCCGTATTCTACGACATTCATATTTGACCCGATCTGTTCTAGTCCTCAAAGTCCAAAAACTTTGACGGGTTGTTCCCCTTTTTTGATCAATTTCTTTGGGTCTTCAATTTTTTTGCTTACAGGCTTGTTTCTATGTCTGAGAAAGGCCTCTGAAGAAAGGGGTTGTTGATTTGAAGATCTGCGAGGTCTGGATGAACGAGAAGTTCAACCAGATTTTTTGTTGTTTTTCTCTCACAAATTGATAGAAATGTTTGATTGCATTGTAGTTTCCATTTTTTGAATTTTTAATTTAAAAGTAAATAAGATGTTAAATGAGTTTTTGACTCAACTCAGGGCTTCGTGTGAGGCGAGACGTGTCCCACTGATTTCTCGTGAAAGCGAACTTCATATCCGTAATCTCCTCCAAAAACATCAACCAAAAACTTGTCTGGAAATTGGGGCAGCTGAGGGGTATAGTAGTATCGTGATTGCAAGCACGATTGCAGAATGGTGAGGTCAGCTTACTTCTTTTGAAGTGGCGTATCCTGCCTATTTGGAAGCACAGAGAAATATTCATCAGGCAAAATGCTGGAATACGACTCTGTATCCGTTTGATATTACGAAATCTGAAACAACAGCAAGCGTATTGCCCGTGCACTGTGATTTTGTATTCATTGATGCTCAGAAAAGCCAGTATGGAACCTATTTAGAGAAAATACAAGAAAATCTTAGCTCGGAAAATATCATACTCTTGGACGATGTCGTCAAGTATCAAACAAAACTAACTTGATTATACGAATTCCTAGAAAAAAATCAAATCAATTATCAGATTTTGGAGAGTGAGCCAGGAGATGGGATGATGCTGATTGAAACCAAATAGCTTTTAGTTTGTGAGGAAAAAGATGAAAAAAAATTTGATTGGAGTATTTTTGCTATGTTGTATGCTGTTTTCTGGATGTGGTGCTAAGTCTCAGAATGAAGCTGTCGATGAGAAGCCAGAACTGGCTCCTATTTGCCCAGAAGGATTTTATTGGGTTGATTCTATCAAATCTTGCATGCTTATTTTGGATGAATAATGTAGGGATATTTTTTATTATTTGGCAATTGATTTTTTCTAGCAAGGTGGGTTTTGGGCTATAGATTGGACTTTTGATGAGGAAGGAAGCAAAGGAAGAGTTGCTTTTTTTGAAAAGATTCTTATAGTTCCCTCGTTTTTTAGTTGTTTTGTATTACGGTTGATGAAAATGTTTTTGAGACGCTTAGTTTTTCTGGTTTTGAGTTTTGTTTTTCTCGGTGGAATATGAAATTTTCTTGCTTTTGCTGCGGTAAGTGTTCCTAGTCAGACTTTTGATCTATGAACGAATGTTGATGGAGTTTGGGATCAGGGAACTCAAATCTTGACTGTTCAGACTCGTTGAGCAGGAACAGGACAAATTGTGGCTGCTAAATGGAATACCATGGTGAATACGCTTAGTCTGAAAACAGAGGAGATTACCAAGGTGGAATTTAAGCAGGATGTTTGGTTTCCAAATGTAGCAAATAGCTTGTTCCATAGCTTTAAAAGTCAGATTATTTTTCCATCAAATCAATATACGAGCAATGTTTTGTCAATGGCAAGTATGTTTTTTGGAGCTGTAAAATTTAACTCTGATATTTCAAACTGGGATGTAAGTAATGTAACCAATATGGGCTGAATGTTTAATAGGGCAGAAAGCTTTAATATTGATATTTCTCATTGGAATACGAGTAAAGTAAATCAAATGGGGACAATGTTTTACTGAGCAAAAAACTTTAATCAACCAATAGGGACCTGGGATACGAGTAAAGTAAAAGATATGAGTCAGATGTTTGGAAATGCAAAGACTTTTAATCAGAATCTTTCTAGTTGGGATGTATCAAAAGTTGAGAATATGCGAGGAATGTTTAACTGAGCAGAGCAATTTGATGGAGATATTTCTACTTGGAATACCAGTGAAGTAACTAATATGGCTGGTATGTTTGGGAATGCAAAAAACTTCAATCAACCAATAGGAAACTGGAATACGAGTAAAGTTACTACTATGGCCTCAATGTTTTATTATGCTCATAAATTCAATCAACCAATTGGCAACTGGAATACGAGTAATGTAACTAATATGCAGTCGACCTTTCATTGAGCAACAGTTTTTAACCAACCAATAGGGAACTGGAATACGAGTAAAGTAATAGATATGTCCTTTATGTTTCATGTTGCAAGGGATTTTAATCAGCCAATAGGAAACTGGGATACGAGTAAGGTTACTACCATGAATTCTATGTTTCGTTGAGCAGTAAATTTCGATCAGCCAATAGGGAACTGGGATACGAGTAAGGTTATTAATATGCTTATGATGTTTCATTGAGCGTTAAATTTTGATCAGCTAATAGGAAATTGGAATACGAGTAAGGTAACTACCATGCAGGCTATGTTTCAATGAGCACCAAAATTTAATCAACCGATAGGCAACTGGAATACGAGTAATGTAACTCGTATGGATTCTATGTTTGCCTGAGCTACTAAATTTGATCAACCGATAGGAAATTGGGATACGAGTAAGGTTACTACTATGTCCTCGATGTTTGGTAATGCAGAAAATTTCAATCAAGCAATAGGCAACTGGGATACAAGTAAAGTTACTAATATGGGTTGAATGTTTGCAGGTGCTATAAAATTTAATGCTGATATTTCTAGTTGGAATACAAGCAAGGTTACTAGTATGTGGCTTATGTTTAATAGAGCTTCAAGTTTTACTCACGATATTTCAAATTGGGATTTCTCTCAAATTAATCATGGTCAATTTTGAGGTATCGTTATTAAAGATGATGCATTAAAAGTAAAAGGTATCTATCGATATCCGAGTGAAAATTATAAAAAGCTTTTGGAGGCTATAGATGCTAAACATCAAGCAGGGATTTTGGACTTTACAAAGCTTAGACATGTTGGAGTTCAGTCTACGTATTGTACTTTTGCTGATTTGAGAGATAGACTGATCTCTGAAGGCTTGAATATTCGGTGACAAGATAAATTTGACTGTAAGCCAAAGTTCACTTTTACTACTCCTACATTGCAGAGCAGCTGAACGATCACAGATACGACTATTACTTTCAAGAGTGAGTTCCCTTTGACGCAAACTGATCTAGATAATGGAGTGCTCTCAGTAGATCCAGTAGGAACAACTGTGCAATACTCGAATTTTAACTGTAGCTTAGAACCCTTAGATGCTACTAAGGTAAACTGTAGTGTTAAGATTACTTCTACACAAGAACAAAGCGATAAAAATCTGAAACTTAAATTCTATAAGAATCTCTGAAACAATAGAATCATTCAAACTTCTGCACTTATAACTGGTTATCTTATTGATACGCAAGCACCAAAACCAGCTCAACTCGATATTGATACAAGCTCAGGTATTCATACTCCAACCGTGACTTTAAGAAGCTTTGAAGATGTCTGAGCAGCAGGAACTGGAGCGTGTAAACTTTCTTATACTGGTGCTTGAGGAATCCCTCAAACGATCACTCCACTTTTGCTTGGAGCATCACACGTTTTGAACTTCAACCCAACAGAAACTGTTCATACGGTGAAAGTAACCTGTTCAGATAAGGTAGGGAATGTTTCAGAGAATGAGATTAAGTTTCCTCCAATTATCGAGTTTAGTCCGAGTAATGTTACAGTTAGTAAAGACGTAATGAGCTGAACTTTTACGGTTTATTCTCCAAGTAGTTTTAAGATTAAGCATATCAACGTTGCAAATCCAGCAGATACTGGAGTAACAAAGATCATTTGTAATGGGCAGGATTTGTGATTAGGAGGAGCGGTTGATTTTGATAATGGTCCAACTAATAAAGTGCAATGTAGTTTTCAATGAATCAATACGAGTGGAATCCTAAAGATTATCGCAAAGGATGCAAATGGTGCTGAAGGTCAAAATAGAGCGTTCTTTATTCATGATACGAATCTTCCTTCTCTTTTGATTACACCACTTGGTATGTTGACGGGAGGTGATCTTGCCTTTACGGTAATCGCTACGGATGATCAAGGAATCGCTGCTACAGGAGTTCAAATTACTTCTCCAGGTGCAGCAATGAATATCCAAAACCTTGCTTGTGTTCAGGTGAATCCTACGAAGGTGCAATGTGATTTTACGGTAAGTGATCCTGTGATTAGCTGAAGTATTAAGGTTGATATCAAGGACCTTGCAGGGAATCAAAACTCAAAGACTGAATCAAATTATACGATTGATAAGACCCATCCTACGATTTCCTCTCTTAGTTTTACGGCTTCTAATCATGGAAGGAAATGGAAGATTTCATTCCAGACGCAAGACCAATGAGGTGCAGGACTCTGGAAATCTACAGAAGCAACAGATCCTGATTATGATGCCTATGCAATTACCTATGGAGTAGGTTTGGATGTGAATTGTAGTGGATATATGAGTTTAGATGGGGTGGCTCCTGGAGCTACAGAACCCTTTACCCTCAACTTTGAAATTGATGATGCAAATCAGAATGGGAAGTATCTTTGTGTAGTAGTAAAAGATAAAGTCTGAAATACTCATACGGGAGTCCTTGCATCAACTATCAACTTAAACCTTGCTCCTACCCTTACCGATAAAACGCTTACGCTTGATGAAAATACTCCGAT", "length": 169615, "complete": false, "type": "contig", "topology": "linear", "simple_id": "contig_2", "orig_id": "GCA_003260355.1|MPSQ01000002.1", "orig_description": "HMT-345 Absconditicoccaceae G1 bacterium HMT-345 HOT-345" }, { "id": "contig_3", "description": "[organism=Absconditicoccaceae [g1] bacterium hmt-345] [strain=HOT-345] [gcode=11] [topology=linear]", "nt": "ATGTTGCTGATGGACACCACAGAACCGCTGCTGCTGCAAGGGTTGGACTTGAGAGAAGAGCAGAGAAACCTGATTACACTGGAGAGGAAGAATTTAACTACTTTATGGCAGTAATTTTCCCAGATAATCAGCTCAAAATCATGGATTACAATAGAGTTTTGAAAGATCTCAACGGAATGAGCGAAGCAGAACTGCTTGAAAAACTTCAGACTCATTTCACGGTAGAAGAAAAAGGAGAAGCTGAATATAAACCTACAGCACTTCATAATTTCAGTATGTATCTAGGCAATAAACGATACTCTCTCACTGCGAAAGAAGGAACCTATGATGATAAAGATCCTATTGGAGTGCTTGATGTGACGATCCTTTCCAATCTCGTTTTGGATCAGCTCTTCGGAATCAAAGACCTCAGAACCGACAAGAGAATTGACTTTGTAGGAGGACTTAGAGGGCTCGGAGAGCTCAAAAGAAGAGTAGATAACGGAGAGATGAAAGTTGCTTTTGCACTCTATCCAGTAAGTATGCAGCAGCTGATTGATATTGCTGACACTGGAAATATCATGCCACCAAAGACAACTTGGTTTGAGCCAAAACTCAGAAGTGGACTCATTATTCATAAGTTATCATAACAAAAAAGGCTGACCTCTCAAACAGGTCAGTTTTTTTAAAGAGAAAAATTTGATGGTCTCCAATAGACTTAGTCTTATATTCTCAGAAACCTATAACTTCTGGAAAAAATCGCCAAAAAAGAGGAAAAACAATCTGAAATCTGACCAAAAACACCGTTTTTAGTGAAGCAACAAAAGTTTCTTCGCCCAAAACGTCTCTGAAAGCACTTTGACAAAGGTTTTTTGACAATACAAGCTCAAAAAAACTGGATTCTAACTCTGAGATCAGATTTCTTTCTTTTGAAAACCTCTAATTAACCAAGCAAAAAAGATTTCCTCAGTTGATAGTCCAAATCCCCTGCCGTAAATACGACAACAATCGTTTTTTCATCAGGAGTAGCCAAGAGTTCTTGCACTTGCTGAAAGGTATCCAGATATTCAGCTTTCACCGCTTTAGCAAAATAAGCTCAAAACTCAGAAAAAGACTGAAAATGCTGCTCCTGAAAAAGCGGTTCATCAGCGAAATCGGAAAACTTTTCCCTTGCAGTATAGATAGAGAAAATCGCACGCTGATCAAAGGGAGCAAGTGCTTTTTGAAAGTCTGCCCGTCCTTTTAAGACTCTATGGATTTGATGAGGCTGAAAAATGGCGAGAAGTTTGTGGTCTGGAAAGCGATTTCTTAAAGTCTCATAAGCAAGCTGGATGGATGAAGCGATATGTCCATAATCAGAAAAGAGTTTTGCTCAACTTGGAAGCGTCGTTAAAAACTCCATTCTCCTCCAAATCCCTTTAAATGACTTCATTTCCTTTTCAATCTGCTCCAAAGAAAGTTCAGGATTAAGTTCCTGGAAAAGTCCCGCGACAAGTGAAGCATTGCAATCGGTCGGTTTCCCTCGGATATAGTCTAAATTCCAATGTTTGAGGGGAATTTGCTTTATTTTAGAAAAAATTTCTGGAGCTTCAAAGGAATCCAAAACGAAAGTCAGATTTTTGATTTTTTGATTCATCTGGATGAAAGCAGACTGATAATCAGCCAAATCACGATAGTAATCGGTATGATCCAGTTCAATATTGGTAATCAACAGGTATTCTAGGTCAAGATTGAGAAAATGTCTCTTGTACTCGCAGGCTTCCAAGAAGAAATAGTATTTTTTGATCAGTTCATAAGGGAGTTTTTTTCCTGTGAAAATAAAGTCAAAAAGCTGACGAAATTCAGGTTTTTTATCTTCCTTTAGATAGTAACTCTTGTTATTCAGATCAGGAACTAGCGCTCCTACGATTCCAAGTCCCAGCTCAGGAATCAGTTTCTTCCCTGTAACAATCGCTAAAGAGGTAGAGCTTGATTTCCCATTTGTCCCAGCAAAACCAACGGTGCGAAAATATTTCGTGATCTCTCCAAGGAATTCAAAATAATTCCAAATCTTCATCGGAAAATGCTCTTCCCTTTTAAGCTGAAAAGCAAATTGGACTTCTGGGCTTTCTTTCGTTGCTTCGGAATAGATGAGAATGTCATCAGCTTTCGGCTGGTATTTTCCGTGCCCAATGATAACTGGAATTTCTCTTTCACGAAGTGCTTGAGTAATTTGCGATTCTTGAGCATCAATAGCGATGAGATTCGTATAGCCAAGATCTCGGAGAAGATGAGCAATATTGGAAATTCAGCTTCCGCCTGCTCAAAGGATAACGATATGCATAGGATTTGGGAAATGAGGAAGTAAAAGGATTTAAGTGGCTACGAGCGATCGTAAAGCAATCTAATGTAGCTTGTCATTGCGAACGAAGTGAAGCAATCCAGTTATTATACTCTTCTAGATCGCCACAGCTTCGCCTCCCGATAGCGGTCATTGCGAACGAAGTGAAGCAATCCAGTTATTATACTCTTCTAGATCGCCACAGCTTCGCCTCCCGATGACGGTCATTGCGAACGAAGTGAAGCAATCCAGTTATTATACTCTTCTAGATCGCCACAGCTTCGCCTCCCGATAGCGGTCATTGCGAACGAAGTGAAGCAATCCAGTTATTATACTCTTCTAGATCGCCACAGCTTCGCCTCCCGATGACGGTCATTGCGAACGAAGTGAAGCAATCCAGTTATGATACTCTTCTAAATCGCCACAGCTTCGCCTCGCGATGACGAGTTTACTCATGATAAGGATTCGCTTTGCAACAATGAGACATTATGATCTGAAAGATGTATGCTATCTTTTCTATTCTTCAGCTTTACCTCAGAAGGAGAGCATTAGCTTGAGATTAGTCAAGCATCTGTTCTAAAATCTGATCCTTTACTTTGCGGATTTCAGCTTTTCTGTCCCTAGAAGATAAGGTTTTTTTGAAGGATTGCAAAGCAAGCAGATGCTTTTTGAGCTGATCAAGGAAATCAGGATCGTTCTGCTGGATCAGAATTCAGTTTTTATGCTCAACATATCGCTTTGCATTGTCTAACTGGTCGTGAGTTCGTGGGATCGGAATCATCAGCAATTTCATATCAAAAAGTTCCTGTTCCGCAAGTGAAGTCGTCCCAGCTCTCGTGATCGCAATATCGGACTGAAGATAAAGGCGACCAATCTCCTTTTGACTCACAAAAGTCAAGGTCTCCACATTCGGGAAATCAGAAAAAAGCTGAGCAAGATCTTGATTGAGTTTTCCAAGAATGATCGTGAAGTGCATTTCTGCAAGTTCTGGAGTTGTCTTCAATACTTTTGCAAGAGCTTCATAGAGATTTTTTGAGCCTTGAGAGCCTCACATTACGAGGACTTGTGTCTGCTTTTTATCTCAAGAAAGAGGCGAGTTTTCTGCTGGTAATAAGTCTTCAGAAAGGATTTGTCCTACGACTTGAGCTTTGGGAAGCACCTTTGGGAAGCCTGTGAAAGAAACCTTTGCGAATCTTGACGCAATACGATTGACCAATCAGGCGTGGACATCGGATTCGTGAACGATGATTTTCTTTCCCAGAAGTTTCGCTGCAAATACGACTGGTAATGCGACATATCCTCCTTTACAGAAGACTACATCAATCTTGAAGCGCAAAAGGTAAAAAACTGACTGGAGAAGTCCAATCGTAAAGAAAAAGAGGTCTCTGACATTTTTTATCAAAGCTCAAATTCCCTTTTCTCTCCTTCGTTTTCAAGAATAAATGGAATGAAAGGTCAGATTTTTGATGCTTTTTTGAAGTTCAGAACAGGTTTCTTGTTCTAGACTGTTTGAAGTTCCAAATCGGAACATTTGAGCGACTTGTCCTTTGAGGTCTGAATGAGTTTGGAGATGTTGGATGAGACTTTTGATTGGGAAGACATGACCACCGGTTCCTCATCCTGCTCGTGCTATATTGAGTGTTTTCATAAAATTAATCATATAGTAAGTAAAAAAAGAGAACAGCCACATGCAAAATCAATCAAAACCATCCGAATTAAAGCCAAAAAAGCTATAATTTATCTAATTATCAATAAGTACAGGCAATTATCAAAGCAAAGACTTGAATACTATTAAACCTTTTTCATGGATAATTCATCTAACATAGATATTTTATGAGATATAGCTCCTCTTTTAGACTTCAAGAACTAAATCTAAGCTGATTCTAGTGAATATCATTCATCTTTTCAAGAGAAAAGCATAACCTCATTCAGAAAAGTTTTTAAAAAAGAAAAAACTCTCAGCCGAAGCCAAGAGTTTAATCCTAAGTAGCAATAATACTATGACTTATATACAACCTGAAGAGTTCCCCAAGTAGCGATATTTGTATAGTTATCATTTACTGTATAGTCATAAGTCGCACCATCAACTACCTGATACTTCAATCCTGTCAACTTAAGAGTTCAAGCTGCAGTAATATTCATCTTAAGCTCAGTTGATTGTCCCTTAGCAAGAGTAACCTGCTTTCCTACTGGAATTGGATTAGTAACAGACTGACCATTAAGAGTTGTTGACGTAGGATCAGCATTAACAGCAACGTAATCTGTGATAGTAATATCATCATCAGATACATTTTCAATTTTAACTACCAACTGATTATCCTTAGCACTTACTACAGACAATTTAGGGAATGCCTTCACAAACAAGTACTTAGAGTTAATAGCCTTAGTAACTGTTGGGAAGTCTCCTTTCACTTCTGCTGATGCAACTTCAACAGCAACACCATTAGTTTGACCATCAGTGTTAATATTCGTTGTGAATACTACATTATGATTTCCTTCGTTAAGAGTAACGTTAAGACCAGTGAATTCAACACCAGCAGCAGTTGAAGTAGCAGTATAGCTATTTCCATTTACTTCAACAGTCACAGACTGTCCATTCAATGCAGCAACGGAACCACTATAGTTCAACTTAAGACCTGTAAGGTTGATTGAACCATTTCTAGCAGACAATGTGAACTTTGTCAATTCAGCATTTGATCCTTCATTCACTACCTGGCTAACAGCATTAGCATTAGCTACAGATACATTTGAAGATTCTACTACAGACAATTTAACAGTCTTTACAGCTGCAGTTGTTGCAGTATTTCCGTTAGTATCAGTTCCTTTAGCAACTACGTCAAATACGAAGTCTCCAGTCTGAGTCATTGTAGGAGTAGCATCAATTCTAAACTTAGAAGGAGTTTTTGTTGCAGTTCCTACATTGTAGAATGTTACAACATTGTTTCCTCTTCTATAAGTTGCATTAGATACAGGAGTATCATTGATATAGAGCTGAAGATCAATTTGTGAACTATCAGTAATTCCAGTAGCATTAAGTCCAGAAAGAGTTACCTCAGTTACATTAATATCTCCCTTAGGAGCAACCATTTCTCCTTCAAAGATTGTCTTGATAGAAGATTCAGTCAATGTTACTTTCTGTGTTGAAGAGGCTTTATTCTTAAGAGTGAATTGAGCTTCTTTTACATTAATCTTAGCAATTTGTACTGCACCAGCGATATCATTTCCTTTCAAATCTTCTGAGTTATTAGTATATTCACCCTTTGCAGAACCGAAAGCCAATGATCCGATTGTTGTTGGAACAAACTGAACTGAGTTATGTGCAGGCAAAGATTCTAATGAAGCGATAAGCTTAATAGAAGCTGTCTTATTTACATATACATCATCAAATACGAAAGCTGTTCCATTTACAGTAGATGCATATCTAGATCCTCCGATTTCAAGAACAAGAGATCTAACAACACCTGTATTATTTACAGCAAGAGTCATCTTAGGAAGCTTGATTGGTTCAGCAACAGTAAGAGTACCATCTGCGATTACAACATCTGAAGATCCACTTCCTGCATCAACTGTTTTAGGGAATGCAGCAGTGTTTGTAAGAGTAACTCTACCACCATCGATCTTGTAATTCTTCATGATCCATGAATTATCCTGAATAGGCTTATTCATAGTAGTTCTAAACTTAGTTGAAGCTTCATAAGCTACCAAGTCTCTATCCTTTCTAAGTTCAAGTTGAACAGTATCACCAACTCTTTCTAGAGAAGCTACTTCACCCATTACAGTATAAACAACTGCTTTTCCTGCGTTGATAGTATCTTCAAGAGTAATAGTAATGTTTCTTCCATCCATAGATACAGACTTAGAAACTACTTTATTATCTCTCATAACCTTTACATTCTTAAGAAGAGAGTTAAGGTCTGCAGTTCCGTTGTTTCTGAAAGTAACAGACTTCAAGAATACATTTCTGTCGTCTCCCTTAGCTTGATTTGAAACCTTGAATTGTCCAAAAGTCAATTCTGATTGATTTCCAAGCTTGTAAACAACATTACCAGTTCCTCCAACATTAGAGAAATCAAGAGCAGCTACATCATATTTAGCAGTTCTATAAGTTGAAGTTACAGCGTTGTAAACAGCATTTTTAGCAGTAGAATCTACACCAATAAATTTGAATGCAATTTCTGCACCTGCGCTAGCTCCGCTAAGTTCTACAACTAGATCAAGCGTTTCTGATGACTTAACCTTGAAACCTTTATTGAAGTTAGTCATAACTTTTCCATCTGTAGCTACAGTTCCTCTAGATGATACAGCGATACCATCCTTTTCGAACCATACACCTTTGATGTCTGATCTACTAGAAAGACCAACTCTTTCCAAAGTAACTGAATTAACAGTTACATCATTAGTAGCATCAAATTTGATAGCGTTGAAGATAGAAATACCAACCATAGGAACAGACCTAATCTGGTTTGAGTAATCAACTACGTTGATATTCAAATCTCCAGCAGTATTATTAGTTCCTGTATTAGTAGTATCTGGTGTCTTACCCTTACATCTAGCAGGACAAGCATCACCACCTAATGCACAAGCCAAGATCACAGCAGGATCCTTACATTCAGAAGGAGTAACTACATTAACTGATCTCATAAGCATAAGCATTACATATCCTCTAATTTCCATTTGATTTGGAGTATCAATCATTTTCATGATTCCAGCATCTCTCAAAGCCTCAAGATGATTCTTGTAGAATGGAGTAGCTCCATTGTACTTGTCTCCCCAAAGAGCTCTAGAAAGAGTTGTTCCAAATTCACCTCTTGTTACTTTATCATTTGGTCTGAATTTGTCAATTCCTTGACCCATCAATCCCATCTGACAAGCTTTCTTTACATAAGTAGCCAAGTCTCCTTCAGCTGTGTGTGCATCTGAGAAAGAGCAAACAGCAGTTGTGTCAACTTTAGTACCCATTTCTTTTTCAGCCCAGTTAGCAATCATCTTTGCCAACTGACCTCTAGTAAGTTCACCGTACATATTAGCATTATCAATAGGGCTCATAGTAGTAATACCCTTTGAATAAGCGTAATTGTAGGCTTCTTGTAGTTCCTGACTGTATGAAGCAGCACCAAGTACTGATACAGGAAGAACTGATGCAAGCATTAACCCTCCAGTCAAGCCTGCGAACATTTTTTTAACGTTCATTCTTTGTGTAAATTAAATATAAAAGCTTTTTTGGTGAGGAATATTCCTCATCAAGAACGTACTACCTGTTATCCATAGAACAACGAATACTACGACCGTCAAAAGACATAAATCTGAATCTTTCCTCTAATCAGAAGCTAAGATTCCGACTTATTGAGTTTTTCAAGAGTAGAATACGAATGTTAATGATCAACAAGGTATGAGTAAGCCGTTTCTATGTCTCCACATAGACAACTTACGAGTGATAATACCAATACCGAGATTAAAATCAAGACAAAGTTAGACGATTTCGACATTAAACTGTCAAAAGCTCACATCTGCTTAGTAAAAGCCAACCTTCAAAGAGGAAAAGCTTCCACCTGTCCTGACTTTAGAGTAGAGATACCTCACTCCTACATCCTTACACAGATGATTACGCAATTCACATTTATTTGTGACTTTTTTTTTATTTTTTTCTCACAAAAACGTAATAAAGCCAATCATAAAGCCACTTTTTACAACACAAAAACAAGCGACAAAGTCCACCGAAAACACTACAAAAGAAAAAGAAAATATTGACAAAATCAAAAAAACTGATCCTTAAGATCAGTCATTATATACAAAATTTAAAAGCTATTCCAGATGTTCACAATAATTTTTTGCAGCATCTTCCTGAGCTTTCTTCTTGTTTGTTCAGAAACCTACCGCAAGCACCTCCTTTTCAGCAATAATTTCAGAACGAAAAAGAAGCACATTTCACTTTTCATCAACCTTTTCATCATAATCATTATAAACAGGCAAGTGTTTATAGAATTTTTGAATATGTTCCTGCGCCATCGTCTTATAAGACTTTACCGGACTTTTGGAAACCTCGTTAATCTCAGGGTAGATACTATCAAGGACAAATTGCTCCATTACAGGATAGCCCATATCAAGTGCTAAATACCCAATCAATGCTTCCATTGCATCGGAAGTAATTGAAGCTTTTTCACGGCCTCAGTTCCTTTCTTCGCCTTTACTAATAAAAAGCTGATGATGAAGTCAGATTTTTTTCGCTACTTTACAAAGAGTTTCCTCCCTTACGAGAGCAATCTTATAAAGTGTCATCGTAGATTCCTGCATCTCAGGGAAATCCAAGAAAAGCCTCTTCGCAATCACCGATTCCAGCATAGCATCCCCGACAAATTCCAGTCTTTCATTATGATCATATGCCACTTTAAAATCTGCCGCATAAGACTTATGAACAAAACAGCAACATAAAAGATCAGAATTCTGAATCTTTTCAAGTGGTAATCACAGAGAGGAAAGGAATTGTTTGAGACGAGGAAGTGCTTCATACACCTCCTCAGGCAAAGAAAGAGAATACATAATAATAATGAATAGCTAAATTATTCACGATGACTAAGCACAAGTTCATCATACTTGAAAGGCTGTTCTTGCAATCCACTAAGATAAGAAGCTCCCAAAATCAGCTGAATATCCGCTCAAGTAAACACTTCTACTTCATTCCCATAGTCGTCGATGATTTTCTTCACCACTCCTGTATTATACACAACCTCATCTATCGGCACAAAGTTCCTCATCGCTGTAATAGTTCCTTCAAACCCTTTTGCCTCAGCATTTTCAGTATTGATAATCAGCCTCGTAAGTGGAAGAGGATCTGTGTTGTCAGTAGCAATCACATCCAAACCATACCTTTTCATCTTAGAAGCAACTCTACTGGCCACCCTCACATTTTGCATCTTATTTGAACGAAGAAGTTCTTTATCAATACCATTATCGACTTGAATTTTCGCATTCTCTAGGAGAAATCTCTGAAGATGGGAAATAAAATCCACATACTCATGAATCGCCTTATAATAGGTAATATTATTAACCGTAGCCCCAAGAGGAAGCAAGACCGCAGCCCCTCCAAACAAATCTCTCTGAGGTGCATAAAGAAAACTTCCTTTCTGAGTAGTTAAGTTCTCATATCCATAAGAAGTATTAAGTCAGAAAGAAAACGGCTTTACTCTATCTAAATACTGAATTGTCCAAAGCATCTCATTGAGTGAAACATTAGTGTTAACCATTGCTACATAACCAGAATAGAGCTCTTTTGCCTTTTCCAAGCTAAGATTTCCTCCAGAAAGCATTTTATCCTTAATTCCCTCAACAATAAGCTGCTGCCTATAAGAACGATCAAAATCTGACGCATGTCCAGCAGCATGCCTAGACCTCGCATACTTAAGTGCTAAGGATCAATCCATATGCTGAAGTCAAGCTTCTACATGCAAAGGTTCATATCCTTTGAGATTAACATCTGGGAACTCATAATCATGCAACGCATAAGGAACATTAACATCAATACCTCCTAAACTATCAATAACTCCCTTAAATCCTCAGAAGTCAATCGTCGCATAATAAGGAATCTCTAATCCTGTAATTTTCTCTAATTCCCTAGCAAAGCCAGATCAAGCCTCAGGCAGACTCTTCGTGCGACCATAAAGCTGAGTAAATACCGCATTAATCCTTGAATACCCTCCAAGTGGATTCTTCACATAGAGATCTCTCGGAATAGAAAGCATACTCACTGCGCCCAATTCTGGATTTCGTGAAGCAACAATGATCGAATCCGCCAAATACCCTCCACCATGTGCAGCGCCTCCGTATCCCACCAAAAGCACATTCACATTTCAGTAAGCATCTGTCTTCATCGGCGTCCCAACAGTCTGACTTACTACTTTTACAGTCTCTTTCGCAACTCCTACAAAGAATCCCTTAATAGTATGTGCAAAAGAATGAATCAGAGCCAAGATCACTACCAGTGCAAAAACGAGAATCCCACATTTTACCAAAACTGAACCCGATGATTTTTTAGTTTTCGTTTGTCAAAGCTGCCTTTTTTGCATAATCATACAACGATAAATAAAAGATACAGTGAGCGAGTATAGTGATATCTCCCAAAATTTCAAACCACAAAAAAAGAAAATATCTTCAACCTTATTTTACCTTTAATAAAAGAAAAAATCTGATTTTTCCCGGAGGAGCGAGCTCTTGTCTGGGAAAGTATTTTTACCTATGACATCAGAAATACTGGAGGAGATAGATTGACAAACTCCACTTTTTCCACCCCCACCACAAAAAAACATTCATTTTTTCTCCAAATCGCTTAGTCTAACCAAATTTTAAAAGTAAAAATGGAGCAAAACGACGATTTGAGTAATTTACAAAAGTGAAAATGAAGTAACTAAATAAAAATCTATCACTTCCACAAAAAAAGCCCAACCTCACAAGGAGGATCAGGCTTTTAGTATTTTTTCATCATTTATCCATTTCTCAAAGCAGCATGTGCCGCAGCGAGTCTTGCAATCGGCACTCTATACGGACTACAACTTACGTAGGTCAAACCTACTCTATGACAGAAATCTATAGAAGCCGGATCTCCTCCGTGTTCTCCACAAATTCCGAGTTTGATAGTTGATCTGGTTGCTCTTCCTTTTTCTACAGCGATCTTCACGAGTTCTCCTACTCCTTCTTGATCAAGCGTCTGGAAGGGATCTTTTTCAAAGATGTTCTTATTTACATAATCTTTGATAAATTTTCCTGCATCATCTCTAGAGAATCCAAGTCCCATCTGAGTAAGATCATTCGTTCCAAAAGAGAAGAATTCCGCAGTTTCTGCGACTTTATCAGCGGTTACTGCTCCTCTTGGCACTTCAATCATAGTCCCGAGCTTGTATTCCACATCTACAGCGTATCTATCCATCGTTGCTTTGGCTACCTTTCTTACGATGTCAGCATTAAAATCTAGTTCTGATTTAAATCCTACAAGTGGAATCATAATTTCTGGATGAACATCAACTCCTTCTTTTTTGAGTTCACAAGCGGCAGAGATCACAGCTTCAGTCTGCATTTCTGCGATTTCAGGAAAGGTAATTGCCAATCTACATCCTCTATGTCCAAGCATTGGATTAAATTCATGAAGAGAAGCTATTTTCTCATGAAGTCTTTCTACACTCACCCCCAGTTCAGTAGCAAGTTCTGCAATCTGAGCTTCTTCTTGAGGAAGGAATTCATGCAAAGGAGGATCCAAAAACCTAATCGTTACTGGCATTCCTTCTAGAGTTTTCATCAGTTCATAAAAGTCTGATTTTTGATAAGGAAGGATTTTTGCAAGCGCCTTCTTTCTTCCCTCAACATCTTCCGCAAGGATCATCTCTCTTACTGCCTTAATTCTTTCCTCAGCAAAGAACATATGTTCAGTCCTACAAAGTCCAATTCCTTCAGCACCAAACTTTTTAGCAGTAATAGCATCACGAGGCGTATCAGCATTAGTTCTAATTCAGAGTTTTCTATATTTATCCGCCAACTGCATTAATTTCTCAAAATCTCCACTAAGTTCTGGATCCACTACTTTTAATGCCCCAGCAAACACTTCTCCCGTTCCTCCATCAAGAGAAATCTGATCCCCTTCTCTGAAAGTCCTTCCATGAACAGTTACCGTCTTCGCAACTTCATCAATAACAAGCTCTCCAGCTCCAGAGACACAGCATTTTCCCATTCCTCTTGCAACTACCGCAGCATGAGAAGTCATTCCTCCTCTCGCAGTCAAGATTCCTTCAGAAGCAATCATTCCTTGAATATCTTCCGGTGAAGTTTCTAATCTCACAAGCACTACTTTCTTTCCATTTTCTTTCCAATCATGAGCAACATCAGCAGAGAAAACGATCTGTCCAACAGCTGCACCTGGCGAAGCAGGAAGCGCCTTCACAAGAAGCTCAGCTTCCTTTTTAGCCTGAGGATCAAGCTGAGGATGAAGCAAACTATCAAGCTGCTCTGGCTGTACTCTAAGTACTGCTATCTTTTCATCGATAAGTCCTTCTCCAAGCATATCCATAGCAATCTTCACTGCCGCAGCAGCAGTTCTTTTTCCACTTCTTGTCTGGAGAATATAAAGCTTTCCTTCCTGGATCGTAAACTCCATATCTTGCATATCTTTATAATGCTTTTCCAGCAAATGATAAATATCACAAAGCTCTTTGTAGCAGTCAGGCATTACCTTATCAAGCTCCTCAATATGTGCTGGAGTTCTGATTCCCGCTACCACATCTTCTCCCTGAGCATTCATCAAGAACTCTCCGTAAAATTTATTTTCCCCAGTAGAAGGATTCCTTGTAAAACATACTCCCGTTCCTGAAGTTTCTCCCATATTTCCAAAGACCATAGACTGCACATTTACCGCAGTTCCAAGAAGTCCTCTAATATCGTTGATTCTTCTATAAATAATCGCTCTTTCATTATTCCATGAGTTGAATACCGCATCAATTGCCATTTTAAGCTGTTGTCTTCCATCATTTGGGAAAAGTTTCCCTGTATCTCTCTGTACGACTTCTTTATAGAGTCTTACAACTTCTTTGAGATCATCCGTATCAAGTTCTGTATCCAGCTTTACTCCTTTCTTATCTTTTACAGATTGCAAAGCCTTTTCAAAGTCATGATGAGGCACTTCCATCACCACATCTCCAAACATTTGAATAAATCTTCTATACGAATCCCAAGCAAACCTTTCATTTCCTGTCTTGGTAGCAAGTCCTACTACAGATTCATCGTTAAGTCCAAGATTCAAAACCGTATCCATCATTCCTGGAAGTGAGGCCGCAGCTCCAGATCTTACAGATACAAGCAAAGGATCATTCGCATCACCAAGCTTCTTTCCCATCCTTGCCTCAAGCGCTTGAAGCTGTTCTTCTACCTGTTGATCCACTTCTGCTGGGTAACTTTTATTGTTTTTGTAGTAAATATCACACGTTTCCGTCGTGATAGTAAATCCTGAAGGGATTGGCAAACCTAAACTTACCATTTCAGCGAGGTTAGCTCCCTTTCCTCAGAGGATTTCTTTCCCAACCCCCTTTCCTTCCGAAAAAGAGTAGACATACTTTTTTGTTTCTGACATATGCTACGATATAGTAAAATAAAACAATTCTGACTATAGGATTTCCTTTCAACTTTTCAAGATCTTTTTATGTTTAGAAAGAGGATTTTTCACCTGCAAAAAAACTCTCTCATAATCATAGTCAAAAATACAAAAAAGAAAAAGCTGATCTCCATCAAAGAGTCAGCATTTTTCTAATAAAAAGTCTATCAGTAACACTCTTACTTCTTAGCCACCGACCTTTGAAGCATTAGAAGCACTCGACCTCTAATTTCTTGCATCATAGGAGTAGTATCCTTAAGGATTCAAGCATTTTTCAGAGCTTCAAGATGTCCTTTCGCCCATTCTTCTCCATCCTGATTATATCTACTTCCATAAAGGACTCTAGAGAATACCGTAGCAAACTCAGCCCTTGTAACAAGCTTATTTGGTTCAAAGCTGGATAATGCTGTTCCATCATACTTAATTCCCATGAGCTGAAGCTGATAAGCAAGCTGAATATATCCAGCCAAATCTCATAACTTCTCATCAACATCTGCATAGTGAACAGTTCCTGTGACAACAGGCTGCTTTCCTAGCACTTTAGTCACATACACTGACATCATCTTTGCAAGTTCTGCTCTGGTAAGTGGCTGATCAAGTCTCGCACCAGAAATCTCTCTAATAGTCGTAATATCTTTCCCACATGCCCAAAGGTAAGCTTGATTCTGTTCCTCTGAAAACTGAGATCCTTCAACTGAGCAAAGCGGAGAAGTAACCTCTCCTGAGTACACACTTTCTACAAAGTTCGACACAGGAACAATGCTATTTCCAGTACTTGATGCTGAAGATGAAGAACTTGAACTACTTGAAGATGATGATGAACTACTTGATCCTCCACCTCATCCTCCTCAACCTCCTCCTCAACAAGTATAGGCCCATTGAGCAGTGAGAGTAATATCTCCAGCAGGCATAGTCGCTGGAAGAGCTGGACCCCATCCAGTAAATCTATAACAGCTTCTTGTAGGATTCGTAGGAGCTGCAACAGAGGTTCCATATCCCTGCACAACTGGCGCTACAGCAGTTCCTCCATTAGCATTAAAGGTAATTTTATACTTCTTTGGCGCATAGAATCGTCCATAATCAGGGTTAGCTGTTTGAAGAGTATATCCTATCACTTTCGTATCTTTTTTAATCTCATGATAAGCCTTAATTGAATGATTCTTATCTACCCAAGATGGAACTACCGCATGATCAAGATCAGGAGTAATACTACCCGTTGCTGTACTAGCTCCTGCAGATTTAAATACTTCTGCTCCAAAAATTCCATCATTCTTTGCTCTAAATACTTTTGTCTGTTTGTTCCAAGCTGTAGCCATATTTACAAACAAAAGCTTCTCTCCAGCCACATCCTTGATTTCAAGAAGATTTGTGGTATCAGTAAACTCTAGGATATTATTATATACTGCAGTCATTTCAGGACTCACACCTGTTACCTTTCTCATCAATGCATGAGTTTCATTGAGCTTAATTGTCCCCCCATCCAAAGCTACAGGAATATAACTTGTATTACTACTACCATAAAATTTAGTTGCTACTGGACAATCTTTTACCTCATATTTTGAGTCAGCATTTTTGATTGAACATTTAGCATCTACAGCATATTCAGCAACATCTTTAGGGAAGGTTCCACCTGAAATTGCAATCTCAGAATTTTTGCCATTTTTCTCAAGACTTCCATTAAAATCACCTCCAAAGATAGCGAGCTTTGCATATCACTCTCCAGAGTTATAAGAAAGCTTAACACCTCCAGCAAGAGATCAATCCTCAATAACTACTTCGGACTTAGATACCCAAGATGGATATTTTTCAAAACCACTAGAATCAATTTGAATTGCTTTTTCTTCTCATTTAATAGTTCCTTTTTTTACAATAATTTTTCCTCCACTACTAAATACATAAAGTCAGAATAATTTTGAAGTATAATTTCATCCATCAACCGTAACTTGTCCATCATTATCTACAGAAATAGCTGAAGAAAGCCACTTTTTAGGATTAGCTTTTTCAACACTAATAGTAGAATTATAAAACTCTGCTTTCTGATTAGAAATGCTTACAGGTTGTCTATATTCAGAATTAATAGTAACATCTCTAAACACAGCCAATGCATCTTTTGCCCCATAAAGATAAAACCCTGGAGTATTATTATCTTCTTTCAAGGTATAGGTTCCACCATTTACTTTTAAAGCAATTGAATGTCACTCATCACTAGCAACAACACGATAGTTTGTCTCAACAACAAGGTTATTTAATTCAACCTTCGTTTTTGAATCTGGATCATTTCCTCTAAATCTAAAGAAAGCTGCATCATCGGTATTCCCTCTATATTCTCCGCCATCTATAACAATTTCTGATCCAATCCCAGCTGGCAAAACATTTATAAATCACCAAAAATCAGTATTTGCAGTTGGAGTGATAACCTTTCCTCCCTTAAGAGTCAGTTTTACAGGGGATGAAACTGTCATCATCCATTGTGGAACATTATTCGTAATAGTTTTCCCATTTAAATCTATGGTTAGATTTTTTGATAGAGATATTGCTGTATTAAGATTAATATCCTTTGTAAGAAGAATCGTATCCCCATCACTAGCAGAGGCTATCGCATCTTGTAAAGTCAAGAATCCCTGATTTCCAATTTTAGCCTCTCCAGCAATAGGAACATAAACATAATCATCTGCTATAAGAAAGTATCAAATAAGTTTACTCCCATTTTTTACCTCAGCATATCTAGCAAAAGAGCCTGAAAGATTCGTCCAATCTCCTTTCAAACTTGCACTAAGATCATACGCAAGAGATGGAGATCCAGACTTAATTACCTTCAGTGCTTTTCTATAAATAGAATCTTTCTTTTCAAAAAGAGGATATCATGATTCATTAAAAATCACCTTTTCTCATCAAAAATCAACCACTTGAGACTTCGTATTCAAATAATAATCCGTTGTATCAGAATATTGCAATTTAGTACCGGTATAATCAACCGTTCCATCATCTTTCATCAAAAGAGGAATCTCATCATCAGAGCCAATAGTAAAATATTTCACAAGCGTAGATCCACCTACATTCATTCCTAAGCTAGAAATTATTCCAGCAGACAATTCACCAGACATATGAGCACCTGTTGCAGACATCAACACATCAAGAGATGGCATCACCACTCCTGAAGCATCTACAACTTCTCCAGTAGCTTGAACTACTTCTTCAAAAACCTGTGCTGCCGTCCCCGTTACACTCTCCTCTGCAGAAGCAGCAGGAGAAGCTAAATCCTCTTGCACAGAAGAGCTCTCCATTGCCAAGCTCTCTCCTTGCACAGTTGAAGCAACAGACTCACCCTCCTCCGCGAGTGAAATCCCTGTTGGCACAAAGATATTAGCCTGCAACATAACGAGCGACATAATCGCCAACCCTTTGACCATTTTCTTGTCTATCATGATAAGGCATAAAAAATAAAAACAAAACATCAGCAGAAAAAACTGACTAGAAAGAAAATTTTTTCCACTGAAAACACTCTTAATATAAGGATGTGACACCCTTAATTCAAGAGAAGTTTTCTCAGTTCTAAAGCACAAAATCAAAACATTACCACTTATCTTTCAATTATTTTACAAAAAATGAGGTAAAAAAAATTGACATTTAAAAAATTTATGATTAAAATAATAATAAATTTAATTATAGCAATAAAAAATGAAAAAGAAATACCTATCCATTCTCCTCTTAGGAACATTACTTCTCCATTTCGGAATAGGAAAAGCAGAAAATTCAGATCAAAAATTAGATTCTATCTTCGAAATCCAAGACTACGTAGAACAAACACTTAATATCAAATCCTCAACAATCTCAAGCAGAAACTATTTCCCTCTTATCAAAGAAATCTTAGAGAAAAAGTGCCCCTCCGAGAGCATAGATGAAGCCAAAAGTACTCTTGAACAAACCCTCCCTACTCGACAAGAGCTAATAAGCCAGATTTCTCCTAATAGAATCAAGTATGAATTTGAAAAAATCAAAACCTCAACATTGCAAATCCAAACAGAAAACCCTACCGAATTTTGCAAAAAAAAATACATCAGTTATACCTTCCTAGAAATGATGAGAAATCAGAATCTTTCTCACTACCAAAATGAACAAGAACACCCAGCTGCCAACCCTAGACAATCGGAAAGAGAAAATTCCACTTCTCAAAACCAACAACCAACAATAGAAGTCCAAAACAATACAAAAAACCTCTCATGAGAGGATCTCACCTTTGCAGAACAAACGAAACAATTCGCACACGAGGAACTAACTAATTTACTAAGTTTAGGGATTTTAAATCCCCACGACCAAGAAACGCTAAATAATCATATTGAAATCAACTATCTCCCTTGATGCAAAAAAACACATGGCTCTTTTCATATCCTTCAAAACAAAACGACCAAGGAAAAAAAATTCAAAACCCTAAAGCTCAACATAGCAATCTGCAACACCCCAGAATATCAAGCAAACTACAGAACATATTTCAAACAAATCCTTGCACATGAATTAGGACATTACATCTATTTTTTCAGGGATCAAGATCCCCAAGCTTTCGATACCATTTGCCGAAATAACGAGCAAAATACCTGCAATGCCAAAGGATTTTTCAGCGAATACGCACAAAAGAGCAAAGAAGAAGATTATGCAGAAAGCTTCGCCTATCGATACAAAAATCTCGAAAAAGAAAAAGAATTCTGATCTGCCCCAACGAGCGAAGCGATCTACCAAAAAGAAAATCATTTCAACCAACTATTTGCCTCGCACTAAGAGCTAGTTCAAATCCGAGAAATCATCCAGCAAAACAATTTCCTCAAATACCTCTCAAGAAGATGCAGAGTATTCAATATCGGGAGGAATAGCATCTTTTTTTGGCAAGGCAGATACAGAAGAATGAAAGCCTGAATTTTGATAAGTAAAGATTTCGTTATTAGAAGCGAAAAGGACATTTCCTCAAGGAAAGAACAAAAAATAACCAAAGAAAAGCGGAGAAACAAACAACGAACATCCTCAGATCAAAAAAGACATTCCCTCAAAAGAGATAAAACAAAACATCACTATGAAGATATCACAAAGAAAATCAAGAGCAAACACAAAAAAAAGACACTCCCAGAATCAAACTAGGAATGCCTTCAAAACAATAATCTCATACCTGTGGAACGACAAAGTATGAGACAGGATGCTGGATCGCATTACTCATTTCCATCGTTAATGAATGCAAAAGCCAGAGACTTCCACGATCATCAGCAACGATATATCGAGTTTTCGCATCTTGAAACCATCGCTCAGGAAGGATAATCATCACTCGCTCTCCCAAAAGAGCCATAGAAACCTTTCGCAGCAAACCTGATTTAATCGAAACTTCTCCAAGACAAAAGAGATTGGAAACGACCACCAGGAGATCGGAAAACTTCGATTTTTCTTCATACTTAACCTCGGGAAAATTAAAAACTCCACTAGAAAGAACACATCCAGAAGAAGCAATTCTCTGACCAAGGCTTGGCAACTTTCCTAAAAGGAAACTACCAGAAATAACCTTTAAAGCAAAAGTATCCATAAATGTTTATTTTTTTAGGTATTTTTTATGAAAAGAGTATTATCAATTTTAAAAGAAAAGTCAAGAAGTAAAAAAAAAGCTGAACCCTATGAAGATTCAGACTTCTTTTTTTGATCAATCTCTACAGAATTATCAAGCAGTTCCAGAAAGAGAAGTAGTTCCAGAAAGAGATACAGCTCCAGATGTTACATCTTCCTCAGAGAAATCATCAAACTCTCCTGAAAATCCTCACATAGCTCCACCTCCCATTAATGCACTCCAAATTTCTTGAAGATTCTGAGCATCAGTAGGCGTAGTAAAAGTATGATTAGCAATTTTCTTTGCAACGAAATCTCCGTCTACATCTACTCCTACTTTATCTATAGTCAATTTAAACTGAGGTTGAAGAGAGAAACGATTTTGACTCAACGTAGCAGAAATCTTTCATGAAAGCTTAAAGAGTTGCTCTTTTTGAATATCTTTTCCAGTTTTTTCAGCTACAAGATCCAACTTCACACTTCAGATACCAGCTCTCTCAGCAACAAGGGTTACTTCCACTTGTCCAGATCCATCAGCAGATGGAATAATTGCCTTAGCTGTCGTTACCTTACCTTTTACGTGCTGACTAAGCTTCATTTTAAGATCACCCATTGAGAAATCACTATTTACTACTACAAAATCCACCTTATCTGCAGAACGAATCACAAAATAAGCTTCAGTATTCTCAAATTTTATACTAGAAAAAATCTGATCCAAACTGTCTTTCATCTGCTGTTGTTGAGCAAGCAATACTTCATTTCCAGTAGAAGCCACTTCTCCAGTCTCATTCAAAGCATAGAAATCAAGCACAACTTTCTTTATTTCCTCTAGATTAAAATCTACTTTCCAAGCAGGATTTCATTCATAAGTAGTAGATACCACTCCCGTATAATAACTTGGATTCACTGCATAAAGTTTTTCATTCTGCCAAATATTAGCATTAGTCTCTGCTGAAGTCCTAAGCATTTCCTTAATTTCTGGAACATCCAAAGTAAGCCACTTATTCTTAAACATTTCAACCATCGCTAACGGCATTCCTGCAGCCTCTTTTGGATTCATCTCCAAAGCAAACTCATTAAGCTTAAAATAGAGCTGATAATCCTTGAGAAGCGTATTCAAATTCACTTTCGCATCCAATTTCTGAAGGTCTCCTGTTTGAGCATCTTTCTGCTCCATAGTAAGATTAAGACCTATGTCAGACTCAGAATCTTTCGTCTTATTATCTGATACACTCTTCACATCAAGCATAACTTTTCCTTTACCTCCATCAGTCTGCACATCTACCTGTGCTTTAAGATTTGATTCAATTAGCTTCTCCTGATCACCCATAAACTGTAATACATCCTTTACGATAGCATTCTGTTTATTATACACTTGTAAAGTCTCCTCAAAAGAGAGATTTGGAGCTTTCCCACATCATGCAAGAGTTATAGCAACGACAGCGACAAAGCTTAAAACTCCAACTTTTTTTAACTGCATTTTTCATACCATGAAGAATTAAAAACCAATATCTAGATCAACTAAATATTTTTCCAGCAAAATATAATCATCTGGGATCATTTGTAAAGCTCAAGTTTGCTGAAAGCGAGAAAATCTAACAAAAAAGGACATATTCTCACCATAATCCTTATTTTTCCATCGTTTACCATCCAAGCGAAACTGTTTTTGCGAAAAGGAAAGTTTTCATTCAAAAGAATTTATTCACCACATTCCAGAGTAAGAAATCTGATTTTTACCTTTTTTCAGTTCTATTTCCAAGGATTCTCACTTTATCCCCGAGAAAGTTCCCTTAAAGACTTTTCAATCAAAACTTCCTGAAAACCCAATTTCTTCTCAGAGATAATCCCATTTAGTCTGCCTAAAATATCGGACACCTCACTCACTTTCTATACTTCAGCTAAGAAATTGCACCTTCTCTCCATACTGTAAAAGATAGTCCTCCAATTTTTCACGCTGAAAAAGGGATCAAGAAAAAATCTGTGGAAATAAAAGTAATTCCTTAGAAGGGAATACAAAAACATCTTTATTCCATGGAATTTTTATCCAATAATGAAGGAGATCTTTCGCAATCAATTGAACAAATTGTGAATTTACACTCCCCTCTCCCTGAGAAAAACGAAACCTTTCCGGATGAAAATATAAATCAGCATCAGCTCCCCTAAATTGAGAAAGAAAAGAAAGAAAAAGCTGGTTCGTCAAACTCGATCAGTTCGTTTGAAGAGAAAGATCTATTTCCCCTCAAAATTCTTGACCAGAATACGCTACAAAACCAGTTAAATTTACACTTACTACGCGAGATTTCGTAGAAGCAAGCAGCTGACCGTCACCAGAAAAGACTGATTTTTCTCCAGCAAAAGCCTCATTTATCAAAAAAATCATTTGATTCATTCAGATCAAAATCCTTTCATTTTCCTCTTCAGGAGAAAGTTTTTTCCAATTACAGGCAGAAAAAAAGAAAACAAGCCCGAGAAAGATGAAGGAAAAAGTGATTTTTTTCATTTTTTCATCAAGATAAAATAGGTTGCTCCCCTACCAAGAGGAATATACAAATTTTGAAATATAAAGAAAGCCCTTTTCATTTTCTTTTAAATTTTTTGACAAATAAAGACTTTCTCTATATAATTGGAATAAAGTATTTTACTTTTAAGGGCAACGCTCATGAACACAGTAAGAGTCGATATTGATTCCATTTTAGCCGGGATTGCAGAAAACCCGAATATTTCCGACCTTCATCTAACCTGCTCCGGAAAAAGTTCATATAGACTAAACGGGGATATTATCAAAGATGAAAAAATTCCGATCATCAATAGCGAAAATATGGAAATCATCCTCAAACAACTCTTTCAAAATAATGCCCAAAATTTTGATAAGTTTATCTGCGACAAAGAAAGCGATTTCGCATATGAAGGGAAAGATGGAACCTCTTATAGAGTAAATGCCTTTTTTGAAAAAGGAAAAATCTGAATCGTAATGAGAAAAATCAATTCTCGCATCAGAACGCTTGAAGAGATGATGTTCTCTAATCTCGCCGAAAGTATAAAAAGGAACGTCCTTGCAGCAAAAAAAGGACTTTTCCTAGTGACAGGATCTACAGGAAGCGGAAAATCAACCTCACTAGTAAGTATGATTGAATATATCAACAAAACAAGAAACGAAAACATCATCACTATCGAAGACCCTATTGAATATATCTTTGAAGGAGATAAATGTAGTATTTCACAGAGACAGGTAGGACATGATACCTGGTCATTCAAAAACGCACTAAAAGCTGTAATGAGAGAAGATCCTGATATCATTTTTGTAGGAGAAATTCGTGATACTGAAACTGCAGAAACCGTCCTTAGCCTAGCAGAAAGTGGACATCTCGTTTTTAGCACCTTGCACACAAGCTCAGCAAGTCATAGTTTGAGTAGATTTATGTCCTTTTTCCCTACAAATATGGAACAAAGTATCGCCGACAGACTTTCAGATAGTCTCATCGGAATCCAATCTCAAATGCTCGTAAAAAGAACAGATACCAATACGAGAATCTGAATTTTTGAGCTCTTATTAAACACGGTCGCTATTAAAAATAATCTCAAAAAAATGGATCTTGGACAAGTTGATTCGACGATCGAATCTTCAAATCCACTAGGAATGATTTCAATGAAACAATACGCAAAGAAACTCGTAGATAGGCAACTCATCAACCCTAAAGACGTAAAACATCTCTTTAGAGTAGAAGAAAGAGCAACCGAAACAGACTAAATCAGAATCTATCTACTATTTTATAAGGTATAGCCCAGAAATAATGCAGGAAACTTATAGCTGAAAAAGTGGAATGAAAAGACTTTCAAGGGATGAGATCAGAAAAATGAAAGAAAATATGCAAAAAACACCACTCATCCAAAAGCAAGCTGAACAATATCACAATAAAGAAAGTAAAGAAGCTGAAGCATTATTGAACAAGATAGAGATTCCTTCAGAAAAACAATGATGAAAACTTGATATCCAGCCACCAAAGAAAATCTGATTCTTCCAAAAGATTAAAAACTATTTATTTTGATAAAAATACCAAACAATGAATCCAGTATTACAGCAGTATCTACCACAAGAGCTACGGGAAATCGCAGCAGACTTCAAAATTCCTGAAGCCTTCTTGGTAAATAACTCAAACCTTATTCAGCTTATCCTAAAATCAAAATCTCTCGCCGAATACGAAGAGAAACAGAATTGGTTTAATCTTTTACCAATCATGTCTCCTGAACAAATAGAAAAACTCAGAGATATTCTCACAAGAGAACAACAAAAACTTGAGGAAATCAACCAAAAATACAGCCAAAAACAGGCTGAAATTTCCGAAAAATACCAGCAAAGTTTCAATCCTGCACTCTATAGCCAAGCACAGGCAAAAATCCATGCCCAAGAAAACGAAGCAAGAGAACAAGAAATGATTGAAGCAGACAATTTACTGACCCAAATGTAAATATGAAAATATCAATAGCCAAGAAAATCAGAATTTTTAGTTTTATGCTTAGTAGCTTAGGAAGTATAGCAATCTTTTCTCTATGAATCGGATACGCAAAAGGACAAGCAGGGAATACCAACGGAGACTTTCTTGAACAAGCATTTACACCAGCGATCCAAACTGATGCTATCGTAGGATGAGATAGTATTGGAACCGACAAAATGAGCGTAGGAAAATACATCCTAAAAGAAGGAACAGAAATAAAAATCTGATGAAAAAATAAAGGAATCAATCGTAGACCATCACTTATTATTAGTATTTCAAAGTTCTTGCTCAGAATGACAATGGTCCTAGCGGTAACGATGATCATTTACAACGGTGTTCATTATGTTATCAAAGCATCTAAAGGAGAAAATCCAAAAGAGATCCTAAACAACATCCTCTATATCTGAGTCGGAATATTACTTGCACTGTTTAGTGTAATCATCATCAGATTGGTAAGTTCTATTGGATCAAGTAGTTTTAATGAGATTTCTTACCTCTCTAGCTCAATTTTTATTTCCTAATCATACCAAACCCGATGGCAAAAAAAATATTCTCCACCGTCATCCTCGCATTGTTTGCCACGCTCCCTGTCTTCGGGATCAAAGTTACCGTTCCACAAGGACACGCAGGAGATGATCTTCCAAAAAAACACGTCGTCGATACGCAAGAGGAAGGAATATTTCAGTGAATCAATATCATCAATAAATATCTCCGATGGTCTTTTGCCGTGGTATGCACCGCAGTAGTTGTTTACGGAGGGTATCAACTCATTACCGCCAATGGAGACAAAAAAGCCATGAAAAAAGCCGTCTGGGCATTAATGGGAGCTTGAATAGGAATTACGATTGCAATGCTTTCTTATACGATAGTAAGTCTGCTGACAAATCTTTAAAAAAAATAATAAATCTGACCACAAAAAACTCTTGATCTTGCTCATCAAGAGATTTTTTTATAAAAAAAAGAAATAGCTGATACTTTTCCTTGTTCTTAGCTGAATAGAGCAGTTTTCCATCAAAACCATCACAACAATTACAAACACAAACGAAAAAATAGAAGCAATCAGCAAAAACTCACCGTGTAGTTTGGAGTAAAATGATGATTTAAATGAAACGACAAAAGTTTTTTCCACCAAAAATAAAGTTTATTTAAAAAAAAATCCGACGAATCACAAGATTCATTCAGATTTTTTATCTCATTATCAATGGCAACTTTCTTATATCTCTATGCCAAACTAAGCAAACTGATTCCTAAATTCTACAGGAACATCCTCGTTTACGATTCCAGAGATATAGTGGTTAAAATCTCACATAGTGATATAATTTTTTCCTCTAGCTAACTTTCTATGCAACGCTGCAAGTTCTGCGGCAGAAGTTTTATTAGACTTAAAAACATAATTAGGATTACTTGAATGATTCATAGCTATCGGGAAAAAAGTATAGAGATAAAGATCTTTGAGAGACCTAAACTTTTGCCCTGGAGAAAGTTGATAATACTTTTTCACATAATCAAGCTGTTGAAGAGCAGACATAGATCTAAGAGCATAGACCGTTGTTCCGACCCTTCTAGCAGTACTCGGAATAAATTGGATCAACCCCGTCGCTCATGTTGATTTATTTACTATAGCTGGATTAAATGTTCCCCCTGTTTCTGACGCCATCAGCGCCATCAATCGATTAGGATTAATATCAAGCTCTGCTGCAATTTTCTCCATCTTTTGCTTAAAAGCTTTCTTCTGTTCTTCTGTTCAAGGAACCTTATCATACAAAAGAAGCCTTCCATTATATCCATGTCTTGACAAAGCTTCATCTAACTGATTATTGTTTATATCATTGCCAGATTCTGGTTTTTGACCAATATTCTCCACAAAATTATTGATATTTTCTGGAGAAGGATTTGGAGAAATAGGATTCACTACAGGGTTATTAGGATTCACTACAGGCTTATTATTTCCAATAGGAGTAGATCCATTAGGATAAATATATCTATCCTGCCCATTTTGAGTTGCTAGTGCTGCATCAGTATATTGATCAGGTAGAAAAACCTGAGCCTCCACACCATCTATCACATCATCTTTATTAAGAGCAACCCCTGAGAGGAGAATAAACGCTAAAGTATCAGCATTAGGTAGTGAAGATAAAGCTTTCGGATTTGAAGCAAAACGCTCCAATCCCTCATGGAGATAAGTATCCACAAAAGCTTCTCTTTCCTGTGGAGAGAGTTTTCTTTTGAGAGTAAGCTTCGTTTTTTCTCCAACCTTTACTTCTTGCACAAAATCTTCTCCCTTAAAAACAGGACTTTTAGTCATTTTCAAAGTAGAAAGATTTAAAAGTTCAGGTTTTTCATCTATTTTTTGAAGAATCTGACTTTTTATCAGAGGCATATCCAGAGCAAACTTCTGCTGAGCAGCATCCTCCACTTTGAGATTATGTTGATCAAGAAGTGTTTTTGCGGTAGTAGCTTGAAGAGCTTTCTTCTGCATATAATTTTTATAAGACTCAGCAATATATGCTTTCTGAGGCTGTTTAAGCTCCCTCTCAAGAATCCTTTTTTTCCAAGCTCTTTCCAATCCTTGAACTCCCCCAGAGAAACCAAGCAAACCTAAAACAACATTAAGAACTCCTCCAAGTGCTGGAGTTTTCCTGATTTGCTTCCAAAGATCAAATCCTAGCGTTGATTTCAAAACTTTTGAAGTATTTCCCAAAACATCCATACTATCAAGTAGTTTATTTCCTAGAGCATCAATATTCTGAAAAACACCGTCCGCCTTTTCCAAGATTCAGAGCATTTTTTTCATAGTCTCAGGAGTTTCCACCATTTTGATAGATCAAAGCTGTTGTTTCATTTTTTCCTTTCCCATTTGGAAAGCCTGCTGTTTCTCTTCATTAGAAAGGTTATTTTTAAGAGCACGCAAATCAAACTGTTCCCAAGTAATTGAATCAAGTGAAATCTGATTCGGTTTTGGACTATCCTTTGTAGTAATGTCTTGCTTCCAGAGAGGATTTTTGAGCACTTTTTGATAAAATTCATAAGGATCATCCAACTTACTTAATTGAGCTTGATTTTTTGGTAAAGCAAGAAAGTCTGTAAGATTCAAAAGTGTCTTTCCTTGACCAAAAGAAAACGAAAACTTCTCAGAAAAATCTTTTACCTGGTTTACAAGATTCCCTAAACTACTAAAATCCATACTAGATAGCTGTCCCAAAAAATTGATATTCGTCTCATTTCCCATTTGAGCCAAAGTCTCCATCAAAAACAATTCCGTAGCAGGAATAACTACCTTCTTGAGATAATCTGGAACTGGTTCAGTCAGCCCAATATTTAGTTTTCTAGCATAAAACTTTGTTAAAGCAATATTTATCTTCCTAAAAACAAACTCTAACCTTTGTTCTGGAGTTTTTCCTTTCAAATCAGGATAATCATCAAATTGAGGATTCTTTAATATAGAAAGCATGGTATTTTTGAGCGGAGTTCCATCAAGAGTATCCAAAGTCACAAGCTCATTCATTCCATAGTTCAAATTTTCTATGGAGGTCTTTACATTTCCTATTCATTCTTTAACAAAATCTAATGAAGAGTCCTTTTTCTCCACTGGTTCTAGTTTTTTATCAGGGAAAGTCTGAATCTTCTCTTTAGTTTTTTGTTGTGAAGATAGAATCTTCTCGGGCACCTTCACACCATCTTTAGAATTTTTAGGTTGAGACCAATCAGTAGGGAAATCAATAAATCTATAATTCATAGGAAGAACATAAAGTATATAAAATACATCTCTAATTGTAGCGAAAATAGCTAAAAAAGCAAAAAATCAAGTTTTTTTTCTTGACAGAGTAGTACTTTTCAGTAGTATGCCTATAAATATCATGTCAACCTTTATTTTCTAAGATTGAACAATGAAAAAAATCCTTGCATTTCTGCTGATCACTTCGCTTTTTTTTCAGCCACTTGCAATTTTTGCAGAAGAAATAGAATCTCATCCAGAACCAACCATTTCAAGTGAAGTTTGACAACCCTGAGCAGAAGAAAATAAGCCAGAAATCCAGTCATCACAAAAAAAATCAGAAGAATCCTTTTCTTGAAACATTGCAGAAACAAACAATTCAATAACTGGAGAAAACATAGGGAATAGCTCTAGTGAAAACATCTCTGAAGAGGAAAAATCATCTCATGAAAAAAGCAGAGAAGAAGCAGAAGAAAGCGACAAGGAAGACTCAGATATTGAAGATAAAGTTTGAAACTTCACACCAGAAAATGAAATCATCAGCCCAGACACCTCTGAAATAGACACTCAAAAAGATGTAATAGAAATCCAAAATCATCAACCGATTCTCCTTACATCTTGAAATTTAGAATCTTCAAACGAAGATGATGAACCAACACTCATCATTACAGAAGCTTTTTTTGACAAAACAAATGCACGAATAGAAATAAGCAATACTAGTCATAAAACATTTTCATGAATGATTGAGCTTACGTGAAACCTTAGCCTAACAAAATGAGCCAACAAAAAACAACCATTGCTCTACAACCTACAAATTCCCTGAGAAACTTCTATCGTACTAACACAAAATAGCAAATATTTCACAACAAATTTTCCAAAAAAAATCATCAAAGATAAATATACCTTCAATTGGAAAAGTTGACTAAATCTGACTTTAACCTATTGAAACAATAAAACTGACAGTCTCATCGTTCACCCAGATTGGACACAGTTTTTGGAAGGGAAAGATAACTCTTTCGAAAAAGTGCTCATAAATGGGAAACGACTAACTACGAGGACAACCATCGACAGAATCAAAAACATAAAGTGAAAAAATCGGGTTGCAAATCCATGAACATTCTTCAAAGAAGCAGAAAATGCAAAAGACATCTCTCTACCCAAGGAAAAACAACCTTCAAGTGAAGAAGAAGCTGACATACCAATATCCTGCAATACTTTTATCGATCGCAATAGACTAGAAGTACAAGAAATTTTCCGAGGAAATAGTGCTTATCCAGGTTTCATAGAATTAAAGTGGACCGATACTCCTGCAAAATACAAAGAAATAATGTTAACAGGAACGATTTTAGCCAAAGAAATAGTCTTAGAAGAAACAGATTTTCGAGAAAAAAACAGCACCTTCATCATCAGTAAAGATGAAACACGATACGACAAAGGAATCGACAGTCAATCTAATCATGACTTTAATCTACTATATCAGACTTGATACCTAACAATTCAATGATTTGATGGACAAACACGACAAGTTTTAGACACCATTATTTTGACAGCAATTGCTCCAGAAAGTTCCAGCTATACAAAAGGAGAAAAAGCAGGATGCTTTCCAATATTTAATACAACTGGAGACTTTTCTCCTGGATTTGAAAAAAAATTCCTAGATTTTTTCAAAATAGACAGCAAACCAAAAATAGAATATATTACCATCAATTGAGGCGGAAGTTGTCACTGCCCATCAAAAGAAGAACTCTGTACTTCAGAAAGTAATAAACAAGAAAAGCCTCCTAAAAGCCAAGAAGAAAACAAAAAAAATAAAAATCAAGAAACCTCAAAAATAGATATTAATACCTACCAAGTCAAAATAGAAGATATAGAATATGATCCTCCTGGAGCAGACACTAACGAAAGCATCACACTGATCTTGACCAAAGGTGAGGAATTAGACCTCAGTCTATTAACCTTGGATATCAACGGAAAAAATAAAAAACTAAAGGGTATCCTTAAGCAAGGAATTTCTGAAACATTTAGAGGAAGTTATTGATTTCCTAATTCTAGCAAAAATGGAGACACCATCACTGTCCATTTAAAATATAAAGAAACTATACTGGATACTTATTACTATAAGATCTCCTCTAAACAAAAAAAAGAAATCCAAAAGCCAGAAGGAATCAAAGTATTCTCAATTATTGACGGCGACACGATAAGATACAAAGATGAAAATGGAAAGTTAGCATCTGTTCGCCTTATCTGAGTTGATGCACCAGAAAGTAATACTGCAAGATACAAAAAAACAGAATGCTATGGAAAAGAAGCAAAAGACTACCTTACACAAAGACTAAAAGGAAAAACCATAGAGCTTTCTTTTGACAAGGGACAGGAAAATACAGACAGATATGGAAGACTTTTGGCATACGTATCACTAGACGGGAAGCTCATTAATGAAGAACTTATCCGCAATGGATACGCAAAAGAATACACCTACAAACAAAGTTATGAAAAACAAGAAGCTTTCAAAAAAGCAGAATATGAAGCAAAAAACGAACAACTAGGAATACGAGATCCAAAAAACTGTCCAGATCCTTCGCTAAAAGATGAAGAAAAGAAAAACAATATTGAAAATCTAATCATAAAAATCCAAAACATTGACTATAACCCCAAAGGAAGTGATAAAAATAATGAAAGTATCACACTATCTATCTTTGATAAATCAAAAATGATAAAATCTTTAGATTTTAATAATCAGTTTTGACTTTTCATCTTAGAAAAAAATGTATCAGGAATGAATCTGAACTTTGAAGATATTGAAGGAAGTGGAAAATTCAAAGACCTAAGCTTTTTATGAAATATTGAAATAACTAATCCTCTTATCCTAAAAGGCGACTTTTGACTCCCAAATACAAAATCAAGCTGTATCGCTCTAGTTCAAAATGAGCATCTTTTTGACATTGCATGCTACGATATTGAAAAGAAAACAGAAAAAAATCCTGACAAAGAAAGTAAACAGAGAGAAAAGGAAGAAGAATGATTATCAGGGATTATACTTAAAATTCACAGCATAACACCTAATCCAAAAGGAAAAGATCTAGGCAAAGAGAAGATCGAGATTTCTTTTACCGATGAAACAAAAAACCTCCAAAAATTATTGCTCTGAAGCGGATTCTATCTCCTAATAAACCAAAAAAATAAAAAATATCTCTCATGAGAACTAGAAGAAGGAAAACCAAAAATTTTCGAATGAAATTTCAATTTCCCGAATACCAATAGCTGTATTTCAATAGGAAAAGGGAATCAAATCTTTGATACTTTTTGTTATACCAAAGCAAAAGAAGGAGTAACCTATACTTCAACCAATGAAATACTACAAGATTTCACCACAGAGGAACGATCAATCATAAAAGCTATGAAACTAATCAAAGAAGGGAATAAATATTGCATACAGTACAAAAATCAGAATTTTACCTGCCGCAATATTCCAAACACAAGGAAACAACAAGAAAAACAACGAAAGATAAAATCCCAAGCACGAGAATCAGCATTTTTCTGATTAGAAAATATGCTCAAAACAGAATATTCTCCACGATACCAACAAACAAAAGTAAGTGAATACTTTTCCCTCGCAAAAAAGATGAAGTCAGATCTAAAAAATAATATTTTCTCAACCAACATAGACGAGGATAGCTATGAATGGAAAGCTTTTCCAAAAATCTTTGAAAAAGAAACAGATCAAAGCCTGATCGATACCTCCGAAAAAATCTTAAAAAAAATCTTCTCCTCAGAAATAAGATCACGCTATGAAGAGAAGAAACAACAACGAATAGCAGCACTTCCAGACACAATCTAAAATAATCTATTCTTCAATCTGGAGCGTTATCTTTTCAGGAACAAGAGCTTTAGGAATAATAATCCACAAAATATTTTCCGGAGAAAGTTTACTATGAATTCTATCAAAATAAATCTGAGCAGGAAGATCGATGATTTGTCTAATTTTTCCTCGATAACATTCTTCTTTCACAGGCAAAAGATTCTCTTTTAAAGGTGTTAAAATCCTTTCTCCCTCAAGAATAAGCTTATAATCTTCAATAGTAAGACGTATAGTTTCCTTTTTTACTCCTCAAAGTGGCATAATAATAACAATTTCTTGAGAAGATTCATAAATATCATAAGGAATCAATGGTGAATTGTCCAATTGCATAGTTTTATCGTCAGGAAATTAGTAAAGCTTCATAATTTTTGTTATAATTTTCTTTTTAGCCTCAGGATCCTTGATATAGTGCAAAACAATTTCTCCCTGATTGTCTCTATCTCACTCTGAGAGAAAAATCAAGTTTCAATTATCAAAAATATTGTACCAAAAAGTATTTTTTTGAATAGTAATAGTTTTGATATTCTTTACATAACTAGATTTGATATCTCTCTTCAAAGGTCAAGTCTGATTGTATCTCGTAAGATACTCGGGAGTAACGAGAGAAAAATCCATAACATAGTCCAAATAATACTTAAACAAAGGAGCAATAACAAAAAGATAAATGATAAACAGCACTCAAGTAACAATATACTTTATATTTCAGAAATCCTCCAGACCAAGATAAATAGGAAACTGAACCAAGATAAACATAATCGTCCATCAAAAAAATGGAACAATCACCTTTGAAAAAAAGAAGAGAAAACTTTTATTGAGAATAAAAACATTCTCTTTTCAAAATTTATCTACGGCTTCTTGGTATGCCTCTTGCTTCCACTCAGTATCAAAGTTAGAAAAAATCTTCATAGTTCCAAGCTAAAGAACTAAAAGAGAGAAAAAGTCGGGAATAATCTCACAAAAACTCTACCTATAAGATGAGAATCTGGGACAAGATAATTCGCTCCTTGATAACAAGACTTTGAAAAACAAGAAAGAGAATCAGAAGTGCTTCCTCTATGATCTCACATGACAAAATACCCATTACTCACTTCAAATTCTGTCTTTTCTCACTGAGGAATCGTTTTTGTATCAGGATAAAGATACGGTTCATCTAAACGGAAACAATACTGCCCATTCGAAGCCTGAATATCAGAAACAGGAGCCTCATTATTACAAATATAGGTCAAATCATCTACAAACTTTACCGTCTCTCAAGGCAAGCCAATAATCCTCTTAATATAAGGAATATTCTTGCCAGGAGGGACAAAAACAACCACCTGCCCCCTCTCTAAAGTTCAAAACCTCTGAGTCAGCTTTTCCACAATAATCCAATCATTAGATTCAAAGGTTGGAAGCATACTTGATCCCACCACCGTGTATGGAGTCCCAATAAAAAACCTAATAAAAAGAATAATTCAGAAAACGAATACCAAGAAAACAATCAAATCAAAAGTTTCAAGTCAGAATTTTAATATTTTTTGCTTAAAAGACATCGATATGAAAAGGTTAAGAAGTAAGAGTAAGTAGAAAAACCATACAAAAAAGCCTTAAAAAGGAACCTCACTATCAAAATCAGAGAGCGGATCAATCTCTACTTCATCTGAGTTCGAAACTTTCGTATCAAGGAAAATAAAATTATCAATAATCACTTCTGTAGAAAATCTATCATGTCCTTCAGTATCCTGCCATTTTCTGGTTCTTAATCTCCCTTCAAGATAAATTCTCTTTCACTTAGTAAGATACTTTCCAAGCACTTCTGCGGTATTCCCATAGGCAACACATCTATGATATTCAGCATCTTCCAAAACATTTCCTTCTTTATTTTTATACTTTCTATTGGTTGCAAGAGTGAAATTAACGACAGCACTTTGAGTGCTCTCTATAGTCTTGACCTGTAAAGTACTCGTAGACCTCCCAATCAACATTACCTTATTCAAATCCATCTCACAAACACAAACAGAATAAAAGCTTCTTCATTCAGTGATGAAATGTAGTTATTTTTAGGAAAAAAGCAACTAAAAATATCTCAAAAATACCCAACTCCCCAAAAATATAGCTCCAAAATCTCCTGCTTTCGCCTCAACCCTAAAAAACCAAATTCCAAAAACAAAAAAAATCTGAATATCACATCAGACTTTTTAGTCTTTACCTAGAGCGGGACTTGAACCCGCACGGCCGTGAAGCCACCAGATTTTGAGTCTAGCATGTCTACCAGTTCCATCACCTAGGCATAAAGGCAAGAAGTAATGTAAAGATTTTCCAAGCAAAATCAACCCTTTTTCATGGAGCGAGTGACGGGACTCGAACCCGCTGCCTCTTCCTTGGCAAGGAAGCGCTCTAGCCAAATGAGCTACACCCGCATAGAAAGGGAGCATTTCCTACCCCCAGCAAAAAAACTTACACCTCAATAATTTCCAAATTTTTCTCTTCTCTCTTTTTCTTAACCCCAGAAGTCATAATAAAATTCGTTGGTCAAATAATCAAAAGAAGCACACCTACCACACAACTCAAATCTCCAAAGTAAGCATCATTCATAGGATCATTGAGAAAAACGGTCTTTCACATCACAATAGCAGAGATTCCCAAGACAAAAAGCGTAGCTCTACTAAACCTCACATGAATAGGATAAATCCCGTAAAAAATCGAGATATAAAGAGAAATAACAATCAGCACAACACAAAAAACAAAATTCATATGTTGATATTCTGGGAGGACGATGGGTTTCTGCATAATAAGTGAATAAATCAAATATCATGAAAAAGTTCCAAAAGCTGTGATAACCCCGATCCTCTTGAGAGTGTCCCTCATAAGCAAGAAACAAAGAAAATAAAACAAAGATTAAAGTAAGCAAATCCTTTCAATTTTCAAGAGCAATGTTAAACTTGAGGATTAATTAATAATAAAAGAATATAAATTATTTAATAAGAATAATAAACCTAAAATGTAAAAAGTTTTCTAAAATTCATCTTTCAGTAGATTAAAAGGTAGATCTCTCAACTTACGTTCAGTGTTAAAACTTTGTGAATTTTATAAAACTTTTGTGAACTCAGACTCATTTTTACAAAACGACACAAAAAGACATGTCAATCATTTGTGAAAAAAGTTAGAATAATTTCTTGAAATATTCCCGATATTTTTCTTTATGGTTTGCTTTTTCTGTTCAGAGTTTTCAGGTGGATTTAAGAATTGAATAGTCTACTTTCCCATGCTGCTTCTGAGTCGCTGCTAATCCCAGATACGGCAGATAGCTCTGAATATACTTCGCATTTTCTCCATACCAAAACGGAAATGCTTTCGCCAAAGAGGAGGTAAACCTGATACTCCATCTTTGAAATGTAGGATTGCTTTTGAGTCCAGCCGGGAGCAAGGCAAGGGCGGCCGCCTTTTGACGTGCTTGATAACGATCCCTAAATTCCATTAGCTCGGGAGGCAAAAACTCTCCTGTCCCAAAAAATGCTACCCTTCCTTTTTGCTCTTGGAGGGAAAAATTCGAAGCACGAGTAGAAAACCTGAGCTGAGATTGTGGTCCTACTTTGATGAGATTCCCTTCTTGATCTTCAAGCACGATAAAATTATGCTCCTCCTCCTGCTGAGAGGCAAATTGAAGAAGATAATCAGTATTTTTGTCTAATAAGAAGGTCTGACTTTTTGTGCTTGCAGAAATCACCTTTTCAGACATAGGAGACTTCAAAAGAATCGTCGTATAGGAGGGCACAAGCCTTCCATCAAAGACCGTAAAAAGAAACGATCATTGCTGCATGATAAATTGCTGCTCATCAAAGGGCTGCCTCCACCACATCAACGCTGCAATGCTGACCATCAACGCGACGATCACCCCATAACAGCTAGAAAAGAAAACCGTCTGCCACTTTTGTGCTCTAAAGGTCATTACTTCATGTACAATCCCATACCCTGCCGCATACAGCACCACAAACTCTAACCATGAAAGATTGATCCCCGGCTGGACGAAAATCAATAAAAAAAGATAGACTTCTGCGATAATAAGAAGTTTTTTAAAGAACTTCGTATTCTGGTCCGTTGTTTCCCAAAGAGCTCCACCGAGCAACCAAAAAATCCCAAACAAAATCCATACCACCAGATGCTCTCCAAACAACGCTTCCATCCCAAACCCTAGCCCATTGCTTACCACCAAAAAAAGAAAGATCCCGCACAGACTTGCAATACTTATCAGAAAAGGTTTCATACGATACGCCATCTTTCTTGGTAAACTAAAGAACTCGCTCTCAATTGTAGCGATTTTTCACAAAAAAACAAATAAGTCCCCTTTTACTTTCCCTCCTTTTTAACCCATCAGAAAAAAATCTGACTCCTTCTTTCTTTTTACAGCATTACGAGTATAAAAAGTTCTTGATAAAGCAGAATCATCTACAATAACTCCTTGACTTTCTCGGAAAAATAGCTATCACAAGGTTTGTTTTAGAGTCATAAAGATGAAAATATGAGAAAGTATTGAATACTCCTCTTGATACTTGGAGTAATGCTCGTGAGTTGATGTCAAATTATTCCTGAGAATGAGAGGAAAACGCAGACTGGAGATGGAGATAGGATCCAACAGGAAAGTTTGAGTGGAAAAAGTACTCAAACAGGAGAGGAAGCCAAACTCTCACCAGCACAAATCTTGAGTTGAAAGGAGGCAAAACTCACTCTTGCCGAAGTGCAAGCACAGACAGGAGATGATACTCCGATTGTTGTGATTACTGAAGCGAATAGCGGGGCAATTGTAACACAGACGGGAGATTCTCTCATCTATAGAAATGAAGTCTATGGATTTCAAGTAAAACTTGGAGAGGAGACTAAGGGATGTAAGCTCTTTGTGGATATAAATAGCGATAATATGCCTGATAATACTACTTTTCTTCTCTATGGAAAAGTATGGACAGGAGAAGAGAGTCCTCTTGCTTGAGCCTTATGGAAACTCTGAGCTGTTGAAATAGGAGGAGCAAGAATCTTGAGCACTGACAAGTACTGGGACCTCTATAATGAATGAGGAGATAGTATGCGATGATTTGACAGTTTTATAAAGGAGACATTATGATACAATCAGAAATATTTCTTCTTACAGACCTTTACTAATGTTGGTCATAAGGTCCTAAGTGATTGGTTACCCAACCTTAAATGCACTCCCTATAAACATGAAGACTGATATGAAGGAAAAGATTGTTGAGATGTCGACAGAATATTATTTAAAGATTTTACGATCTTAAAGTTCTAATTAGGATACTCTCCCGATAATTTAAGAGAAAAGGCCTCTATGAGATTCCATAGAGACCTTTTGAATACTTAATAATTACTCTTTCTGATACTCTTCCTGAAGATAACCTTTTAGGTAAGAAAAAGAAATAGACTGTTCTGAAGTATTTCTCACGATCACAAGAGCATGCGGTTCTGAAATTCTCACCGGATTGATAACTAAATTTAAGCACTTACTCGGAGCTACTAAATAAGAAAAGTCAACTCTGAGACAAGAGCTCCTTTCTAATTCCAAAACTAGTGGCCAAAAAAGCTGATTATCAGGATAAAATCTTTCATCTTTCTCCTTATAGAAACGATAATTAATTCGTAATCCTGTAGATGAAGAAAAATCATAAAGAGAAAATCCTGTGCTGAATCCTATATCTCTCACATCTATGGCAATACTTTTCTTAGGAGGGATGGTAATCCTAGTTGATCAAGAAGCTGGTTCTAAAAAAAGTTCAGCATCTTCTAAAGAGAGATAACTATAAATAGAAAAATCTGATTGCAAACTCTGCATTCCAAAATTCAGATTTTTTATGTTGAATCGTAGCTGTTCTCTGATTTTAGGGTTAATCAAAAAAGAAAAGCTACCGATTAAGAAACAACATACGATAAAGAAAATAATCTTTTGACATCATTTGAGATGCATGGCATCAAAAAAACAAAAATAAACATAGAGAGACGAGAAACCAGCCCCTCTAAAAGCATCACTCCAATCTCATAGAAGTAGATACATGCTCATTATCACAGAGTAGCTTCTTGTAGTAGCATAACAGTAGCCAAATTCAATCTTATCTCCCTTAGCTACCAAACTTTTTAAGACAGGACCTTTCTTTTTTCCTTGACTTACGCCAACTCAGATATAATTTTTCCCAAAATAATTGCAAGCCATACCATCCCCCCTTTTTCCAAAAAAAAGACCCTGAATACCCTTCAGGTACTAAACCTACTTTGTTTCAAAAGGGGATGTTATTAAGGTATGTAACCTTCTAAACACTTCCAATTGGAGATCTCAAGCAATACAAAGCGTTTGTTGCCGTTTCTTCTTGTTGTAATCACACCCAAAATACTGACTCCTTTCTTGCGGACTCCGTTCCTCAAAAGCAAACCGTGTATAAAAGCTAATCAAAGAAAGATTAGCAAAGAGTAGCAAAAGGACGATGGAACCTATCACTTTTTTCCATCCTCCATACTTAAGAAAATGAAAGATTTTTCTCAACACGGTCATGATATCGTTCCAGAGCATTATTATGTGGATTCAGAATAAAAGAGGTAGAGTTAATGTAGCCATACCTCCAAGTTCTTCTCTTTTTTATACCATACGTTATTTTTTGTTATGCCAACTCAGATATAATTTTTCCAAAAATAATTGCAAGCCATACCATCACCCCTTTTTCCACAAAAAGACCCTGAATACCCTTCAGGACCTTTTCTTTTATCTAAAATAAAGCTCTTATCCTTCACTTTCGTTCTCTTTTTCATCTTCCAAATCCTCAGGATGCAAATGCACCAACCCGTTCCTCTGCCTCCAAGCCAATTTCGTCGCTTTGATCAAACGATTCAAATCCGCTATCGCTTTCTCGCTCTCAGGTGTAGGACTCGCTACATTCACAGCAAAAAGTTTCGTGCTGAGTGTTTCATAGTGTTCGTCAAGTTCCTTGCGGAGGGGTTTGGTCACGATCATCAATGTCGCTTGCCCTTCATTCGTGCGCGTGTCGAGTAAATTTTCATAGCTTTCATTGACAGTTTTGAGCTTTGCCAAGACCACATTCAGCTTTAAAGCCTGTAAATGTGGGGTATACTTCGCCTGCTCAGCATCCAAAAGTAAACTCTTGATCAGCTGCGTTTCCTGACGGTTCGCTTTCCCTTGAATCCCACGATACACTACCAAAAAATCCCCCAATGCAGTTGCTGCCGCTTCCAACGCTTGATCAGGGGAACGCATTTCTGCAGCAATCTTAGTAAAAAAATAAGTTAAAAGCTGATCACGTTCTTGGTCCAACTTGTTGATCTCCTCAGAATTGGGATCTTTTTTCAGTTGCCCATTCGCGTCAATCATTTTCTTGACATTTGCTTTAAACGCTTTTCGTGTTCCGTCTGTAACCTCAGAGCAATCTAGAGGAACAGGAGAACTTTTAGCTTGGGAGAAGGCGAGGCACGATTACTCATACCTTGAAGGAAAGGTAGAAGAGGCAAATCAATACCTTACCAAGAACAAGCTAGAACTCCAAGATCTGAAACAGTTGCATGAGATAGGAAATCTTATCTTTGGAGATTATCTTTCTAAAATTATGATGGATTTTCAGAAGAGGAGCTTAGCGTGGCTTGATGATAAGCTTTTTATCAATGAGTATGCAAAAAATAACATGTATGCTTTTTTGATTATGGATTTTCATGATGATAAGAGGTATTCTAAAGAGGTGTTGCAATCTTCTCCAGATATTCATATCATGACAAATCACATTGGCGAGAGAAATGGATGGGTCTTTATACCCATTAGAGTGTATTTCCCTGCTCCAGCCGAAGTTGCAGATGATCCCACATTTGATGAAAATGGTGGAAATGATACGAGATGGAGGTATTATGTGCTCCAACTCAAGAGAGACGAGAAATCACCTTATCATTGGAAGGTTGCTTATGAGGAACAAGTCGTAAGTCGTGGGATAGACGGAGAAATTCTGCGAGGTGCATGGTTTGCTACTCTGAATCCACAAGATGACTTTTCTGCTAAAATGTGGAAAGACGTTCTTTCAAGAAAGGATTATTGGGAATACTACATTGGAGAAAGAGACTGTCCTCGGACAGGAAGTATTCTCTGTCCTAAAAAAGAACCACTATCTCAATTAAGAAGTGGATTGTATGATAGACAAGCAGCAGCGCGCTATGCGTATTATCATGCATTATCTTATAATACTGAGTATGCAAAGTTTTCTCCTAATGATTGTGCTAATTTTGTTTCTCAGTGTCTCTATGCCGGAGGAATCCCCAAAGATAATACTTGGGGAACTCTTGATGGACGCTATCCTTATTATGGGACATCTACTTGGCTGGGAGCAAATGCTTTATACGAGTATATGAAGAAACATTATGGATATATTGTTTCTAAAGAGGATCTTGTGGCAGGATATGATGGGGACAGAGATTGTGTTCTAGAATGGGGCGACATGCTTGCAATGAATAGAAAAGGTGGAGTTGATATTGATGATGCTGTTAGAAAGACACATGTAATGATTATCTCCCATTCATATCATTACACAAATCAGCATTATAATGGAGTTCCTAGGTATAAGGTCTGCGGTCATACTAATAATAGATTGGATCTTGATATAATGTCACTTCCTGGAGGAAGGGAGTACGTGAAAAAACACATTATGGGCATCCATATTACCTACTGAATATTAACCTAATTAGGAGAGGGAGGAGTTTAATCCTCCCTTTTTTCATTATAAAAAAATCCTGATTCCCACCAAACGCTTGTTTTTAGCAAGGAATTCCTTATCCTCAGCAAAGATTTATCTTGTGAATTGTAGCAAATGAGGAAAGGAGTAGTTTTATTAGGGATGCTTTCAAGTGCTTTGGCAGTGATGGGATGTTCATTTTCTCAACAGCCAGTTCCTCAAGAGGAAATGCTCTCTCATTCAGGGGAACAGCTTACTCAAGCATTCACCGGAGGAACCTGTGATACACCAAACCTTGCGGAATTGCAAAAGCCTATTCCTCCCTGTGAACCGTTAGATATCAAGCCTTTCCCTGAAGCGGACATCAAAAATATAGAGGATGTTGCAAGGAATTGTAAGTATCAGAAAGGACATTTCTTTTATGCTCCTTTTTGAGGAGGGGGAGGAATGAGTTCTCCTATCTCTTTATGGAAGAATCTTATTACCAGAGGCTGAGTTGTGCCCTATGATCCGGAAAAAGGAAAGGTTCTTTGCACTGACGGTGACTATGACTATGAGGAACTGAGACGCCCAAAAGAAAAAGCCTTAGAGTCCTTTGTAAAAAAGCACGATTTACAGCATAATCCGTATTTTACTTCCCTTCTCGTTAACTTGGACAATAATACAGAAGATGAAATCGTGATCCAACAGATGACAAAGTGTGAAGAAATTGAGCTTTGTAGAGATTTAGTCCCAAAAGTTCGAGGAGATAATGAACATCATTTCTTCTTTCATACGCAGGCTTCGATGACGTGAGAGGTTTGGGATCCTATCGTAGTCGTAGATAATAAAAAAGTGTATAGGCTTGGGACGAGCAGAGAAAGTGAAAGTTATAATGAGATTACAGAACGTTCTGCATGGGTCGTCCATGACCTAGTGGGGGACAAAATGATTGTAAAAAGGCTGACGAACTGAAAACTAATCGAGCCTTACAGAAGTAATACTTCGCTTATTGATACGGAGGAGAATTACAATGCTGCGGTTGAAGGCAAACATCTGATTATAGGAACCTTTAATATCGAAACCTGTGAAATCCAACTCTAAAGCAATTCTACGAGAAATATTAGAGATAGTCCCTTCGTATTTGCTAATTGCAGGACTTAGTGCGTAACAATGCTTCCCAAGAATAGTCCTGATCCCAACATTCTCTCACCAATAAAGAAGTCCTTGCTTTTGAGCAGGAGATAGCTATTTTTTATTGCAGTTTGTGGTCTCGCTTTTCACGAGAAAAAGTCAGACTTCTCCTCTTTATTTTTCATCATAGCAACGATGCAATCCCTTACTTCTCAGGAAATCAGACAAAGACGATCAGATTTTTGGACTTCTAAAGCACATACGCATTTACCAGAAGCCTCACTTATCGCAGACAAGGAATCTACTGCGCTTTTCAATGTTGCGGGAATGCAGCCGCTTATTCCTTATCTTGCTGGGAAAGAGCATCCGCTTGGACATAGGCTTTTTAATATCCAAAAATGCGTGAGAACGGTGGATATTGATGAGGTTGGAGATAGTTCACATCTAACTTTCTTTGAGATGATGGGAAACTGGAGCTTGGGTGATTATTTCAAGCAGGAGGCAATTCAATGGAGTTATGAGTTCCTCGTAGAGAAGCTGAATTTTGATCCTCGTAAATTAGCCGTAACCGTGTTTGAGGGGAATGAAGATGCTCCGAGAGATGAGATCGCTTTTGAGGCTTGGAAAAAAGCAGGGCTTCCTGAGGAGAGGATTTCTTTTTTGGATGCTAAGAATAACCGATGGAGTCCAGGTCCTGTTGGTCCTTGCGGTCCTGATACGGAGATTTACTACCGAGTGGGGGAATCAGAATTTCCACCTGTTGGTTCTAATGTAAAAACCGATGAAGCCAATTGGCTAGAAATCTGGAATAATGTATTTATGGAGTTTTATAGAGATGAAAAGGGAATCCTGACCAAGCTTTCTCAGCAGAATGTAGATACCGGAATGGGACTAGAAAGAATGTGTAAGGTTCTTCAGCACAAGGAAAGCGTTTATGAAACCGATCTTTTCGCCCCTTTCCTGCATATGCTAGAACAGGCAACCGAACTCAAGTATGCTGACCACCAGAGAAAATTCAGAATTATTGCTGATCATTTGAGAACAGCGTTTATGCTGATTAACGATGGGCTTACGCCTTCCAATGTCGGAGCAGGCTATGTTTTGAGGATGATTATCAGGAGAGGGTATTACAATCTCTTTTTGCTTAGAAAGATGATGAAGCCGGAGCTGGAGCATTTTATAGATCAGGCTTTCGTGGCGTTCAAAGGGCTTAGAGAGTTTAATGAGCCGATGATCAAAAAGGTGCTCCTTCAGGAGATCGCTCAGTTTGAGAAAACCATTCATAATGGAGAAAAAATTCTGACTGAACTCTTGGATAAGCTTACTACCGAAGGGAAGAAAACGCTCGGAGGAGATCAGATTTTTATGCTCTATGATACCTACGGTTTCCCATTAGAGATTACGAAAGAACTTGCTGCGGATCGTGGAGTTGCTTTGGATCTCGCAGGCTATGAAAAGGCTTTAGAAGCAGCAAAAGAGAAATCTCGCCAAGGAAGCAAGGCGATGTTCCAGAAATCTGCAGATTGGTCTAAGTATTTGGAGGGAGTTCCTGCGACAGAGTTTGTGGGGTATGAGAACCTGAATTTTTCTGATGCGAAGCTTTTGAAGGAGATTGATACCGAAGAGGGGCAGAAAGTGCTGATTTTTGATAAGACACCGTTCTATCCTGAGATGGGAGGTCAGAATGGAGATGCTGGAGTGATTGAGCTAGATGATGGTCGCCGTTTGGAGATTGTAAATGTGCAGAAGGTTGCAGGCGTGATTTTGCATTTTGTAGGGTAGGATTTAGCTTTTTTAAAGACCCAAGAAATGCGATTAGAAGAAAAGCTAGCAACACTCTTCCATCACCAAAAAATTTCTGCAGTGCTTGATCTCGGAGCAGGCGATGGAAGGTGGAGTATTTTCTGTGCGAGCTATGGTGCGAAAGTAGTCGCAATCGACAATCAATCCAAGCCTGATCGGCAATTCCCTGAGTATTTGACTCTTCATCCAGCGATACATTTCTTTCAAGCTGATTTAAAGGAGTTGCCTCGTCCTTGCGACCAAAGATACGATCTAATCTTGCTCTTCAATGTTATCATCTTTCTCAAAAAGAGTTTTGTTCTCTCTCAATTGCTTCCTTATGCTCTTTCACAGCCGAATCCTGAAGGAAGACTTTGTTTGACCTTTTTCTTTTTCGATGATCAGACGATGGGAGTCAATCAAAAGCTTTCTTTCTATACCTTTGAGGATTTTCAGCCCAAAGACATCAGTGAAGAAATTTAGCTTGAAATTCTCCTATTTAGCGGCGATGATCGGAGGCACCATCCCGCTTAATATCTTATCTTTAGGAAAACCAATTCTAAAACCAGCAAACTTACCACTACCATCAAAAGCGTGAGACAGCTTTTCTTCGCCTTTTTATCTCAAGATAAAAAATGGGTACTGACTTAGTATTGCATGATTTGGAGCATCTTTTGCATTTTCAGGATTTTTTTCTGGTAAGGGAAAACCTTTACTTTCGTCCCAATAAAAGAGCTAACAAGGAAGAGGTGTAGCCTTGCTGTAATCACCAATTCTGCGTCTGCTACTTCTTGGACAAAAGCATTAAAGTCTGACTCTCGATCCACAATCGGCAAGGTGATCCCAAGTCTTTTTTCTAGCTTGTTTTTGTACAAAAGATCGTTGTATACAGGAGAAGTGCCTTTGCTGACAGGCACATATTTAAGAGAAAACCCTGTAGCGATAAGAGTTTCACACTCCTTTATTAGCTCTTCAAAAAACTGTTCCCCATTCTTGTTTAGATTGATCAAGACGGTTTTTTCTGCACGAGGAAGAGACTCTACCTTGTCTCGAGGATAGCAAAAGTAGCTCGTATCCATAAAAAAGTGGGTTTTGTGGTATCAGAAGTCCTTCAAAGCCTCAACTGACTCTGGATCACGAGCAAAAACTCCTTCCGTCTGCGAAAGCAACCAGCGAAAGAGCATCTGATTTATCTTTTTTTTGGGGATTTGGATTCCTCCCATGAGATAAACAGGAGTCTTATTGAGTTTAGCTTGCAAAGGCAATAATCAGAGATTTCGATATCGATAGGAATGCACACTTTCTTCAGTCAGGATTTCTCCACCACCGATAATCACCGCATCAACTCAACGGTAAGTCACGAGTTCTTTCCTCTTATCAGAAAAAAAATAGCGGAAGGCTGAACGGAATCCTTTGGGAAATTCGTGGATGTAGGTAATCGGTTTTAGGTCAGGGAATTGTGCGAAAAAGGATTGCAATCGCTCTGGATTATAGGCAGAGACGATTACTTCGTTATTCTGCTCCTGCAAAATACGGATCGTCCCCAGCAAAATAAGCTCGTCACCCATATTTTTATATGACCGATTTCCGAGGATCAAAACCTTTTTATTCTTTAACGGCAAATCTGATTGGAAAGTTTTTGCTAAAGGCATACCGAAAAAAAAGCGTAGGAATCTAAAACTATTGAGTCAATTCTCGTTCGATCACCCTTAAAAACTGCAGAAAGAGTCCATTTTTCTTTCCATTTTCAAAAGATCATGGCATTTTTTCATAGAAATATTTTGGAGCAGCTGGGAGAATATCTCCAGATACAGAGCTTCCACTGAGTCCATTATACAAAGCAGAAAACTTTAGCAAGTCGCCTGTGCTAAAATTTTTGTAACTGAGTGAGATGGGCGTTGTAGGATTCGTTCCATTATATCGATCAATCTTTTTCTTTGAAAATTCAAGGAAATCCTTGATAGTCGTCTCGAAAAGCTCTCTTGTAATCATTCCTGTTCCGTAGTCTTTGCTCACAATTTGGAAGGGACCGTCTCCATTTTTAGGCAAGTAGTAGCCACAACCATTTTCTCTGTACCAAACTGCAATCGTTAAAGCTGTCGGGAAATCATAAGCGAAACTCAGATTATCAAGGGTTTGATACCAACCGATACAATTCTCATGGAGCGGCTCTGCAAGCAAGAGTCCTGAAGCCTGATAAGGCAAGAGAAAACCGGCTTTGAAGGTTCTGCTTTCAGCCTTTGCGATGTCTTTTCTACTTGAGAGTTTTGTGAATAAATAATCTCTCAAATTCTCAAGATAGTAAGAAGTTGATCCTTCCTTAAACTGTGTTTGCAAATCTCTAGCCTGCTGATAGAGACTTCGTAGAGCTTGATTATCGTTTTCAACAAGGGGAGCAATCTGCGACTTTAAAGCAGCAAGCTCCTCCTGATTTCCTGTGCTTGGCTCAAAGGCTGCAGTCCATCCAACAAATAGCCCGATACAACTCATAACCATAATCCAATTTCTGATTTTCATTCTCTTTTATACTGTAAGTAAATAAACTATCTTCCCTTGAAGATCACCCCAAAGGTTCCGACAATCACGTAAATATCAAGCCAAAGCGACCAATTCTGAATATAATACAGATCTAGTTTTGCCTCTTGATCAAAGCTTAAAGAATCTCTTCAGAAAACCTGAGCATATCCAGTGATTCAAGGTTTTATACTAAGGAGCCTCCTTTGCCAAGGCGTATATCTTGCGACTTCGTTTTCTAGGTGTGGTCTTGGACCAACTAACGACATCGTTCCTCTGAGCACAGAGAAAAGCTGTGGCAATTCATCTAAAGAGGTCCTACGTAGGAATCTTCCCACTTTGGTGACTCTCGGATCATTAGCAATCTTTGGTAAGACGCCCTCTCTAGTATTTGCATCAGAATTGATAAGCTTTTTGTAGAGTTCCTCGGCTTCCTTTCCTCCATATCCTACACTTAAGTGTGTATACATCGTGCGGAACTTCAAAAAAGTAAAAAGCTGACCTCCTGTTCAGACTCTTTTTGATTCGTAGATGATCGGACCTTTACTCTCGAGTTTGATTGCAATCGCAATGACCAATAAAACTGGAGAAAGCAGAATAATTCCTACTACTGCTCAGACTACATCAAATATTCTCTTTACCACAAGAGATCGCCCATCTAGCTTTGAATGTTTGTATTCAAGAGCGATGATATTTTGGATAATCTCTGGCTTGTAGACCACATCCTCAAGAAAAAATCCCTCACTAATATGAAAAAATCTGGTATCAAAGAATCTTGTCTTTTCAAAAATTGCTTGAAGCTGATCTTTCTTGAAATTTCAGACCACAACGCACATTTCATATTGTCAAAAATCTGTTTCTTCAGCCTCATCAGGAGAGATAAACTCTGTCTGGAGTGAAAAGTTTGCTTTAATTGTCTTTAAAATTTCGTAGGTATCAATCGTCTCTCAAGAAATAATCAGGATTTTCTTTCAGGATTTTTTCTGTTGCTGATATTCTAGGGCTCTTCGTCCTTGGTCAAAGAAAAATAGTACAATATAGGTCAAAAGCCCTGTAAAAATAAGGATAAACCTAGAAATCCCAAAAAAGAAGATAAAGCCCTGACCAAAATACGAGAGAAACGTGATACTGATGAACCAATAAATCCACACTTTGGAAAAGGTCTGGAAATGGTTGATGACTCTCTTATTCAAGCAATAAAAATCCTTAATAATCCCAATACCAATAAACCCCGCACTTGCAATGATTGAAAATATTATCAACTCCGTCGCATTGATAAACGGAATCGGAAGCTGAACTCCTGGGATAAAATCAGTCCAAAACCTGACTTTGTAGGTAAGAAAGAACAGGCAATTGATGATAAGAAAGTGCAGAACGGGTCTCGTAAGGCGGAGTTTAAACATTCGCTAGCAGAAAGGATAAAGAAAGGGACAGATTGTGCGTGTATTAACGTGTATAACGAGGGAATCTATCTTCTATAGTGCCTTTATCATATCAAGGAGTATAGTATTTTGAAGAACGAAATCCAGAGAAAGTAAATGAAGTAAAAATTAGGGGCTGTTCCTCTTGATTTTTCCTCTGGTTTTGCTATTACGAAGAGGGAAATAATTTTCACAAGTTTTTTTTGCGTAAAATAGAATGAAGTCAGATTTTTTATCTTTTTTATTGGAGCTTTAGGAATGAAACCGGTTTATAATTTGCTTGAATACCTTGATGCTGATAAGGTGTACACCTTTAGTGATGAACTTGATAATCAAATTGAAATCAAAGTATATATTCAGATTCATAGTGAAAAGTTTGTGGAGTATCAGAAAGAGATTTTGCAAGAGTTTGTAGCTGATTTTACAGACAAAGCGAAGGATGTATCTCTTTCTTCCAGTGATATTGAAATGATGCTTGAAGGACAGCTTCAACTCCTCAATACTAAGCTTCAATCTTTTGCGGATAAGCTCCGTGATGCTCCGAAATGCGACTTGAGAGGGTATGTGCAGCTGATCTTAGATAATAGCGTGAAGACCTGGATGATTGGGAAGACTACGCTTATGATTTTCCGTAATGATAAACTCTACTCTGTCCTAGAAAATTCATTCCAAGAACAGACTAACATTGATCAGTTTTCTGATTTTATTGGTGGGGAACTAGAAAGAGGAGATGTTTTTCTCTATGCTGGGACGAAGCTTTCAGAAGTCTTGGATCAGAACGACTTTTCAGAGATGGAACAAGTACTTGCCAAAGAAAATGCAAATGCGATGCTCGATCATCTTGATGATCTCTTTGCTTCGAGAGTAGAAAAGAAAGGAATCTGATTTTTGGGAGTGTTCAGCATCACAGGGATTGATCTCTCTACGATTAAACCTAAAGGGAAGCTCTCTGCCGTGGCATCAAAATATACTTCTAAACTCGGCGGAAAGCTTGCATCAAAGGTTGGCTTGATGAAGCGAAAGAAACAGACCAGTCAACTCATTAGCGGGCATAAGTACTATCTTATGGTAGGGGTGCTTGTTGTTGCTATTCTTTTCCTTGGAGGTGCGGTCCTTTCTCAGCTAAAGTCTGCAAAGAAAGAAGAAGTGTATTTCAAGAATGCTAGTGGAAGTCAGGAACTCATTACTATTGATAGCATTAGAAATGATATCCTTGCATTCCAAGCTATGGATCCTTCCTCAGATGAGAAATCCCTTGAATATAACAGAATCTTAAGCAAGCTCAGTCTACTTGCTGAAAAGGGGCTTTGGGAAAAAGATGTGAAAGATCTCAAGAACATCATTGATACGAATTATCAGAAAGGCTTTAATGTGAGTTTGATTACAAATCTCTCTCAGTTTGATGATGAAGCAACCGGGAGAAAAACCAGAATCCTTACTTTTAATGATGCGGAAAAGGCAAAGCTCGGGACTCCAATCAGTATTAATATTGGGGCAACGACAAATATCGGAGGAACCCAGGCCGCTCTCATTGGTGTAATAAATGATGCTACAAGAGGAAATCTGATCGAATATAATGTTGGCTCTGCGATGAAAGACTGCGAATTGAGCTTGGGGACAAGAGGACTCTTTTGTTATGCTGAGAATGGGGATTTATTCTTCGTGAACAAGCTTGGTGTTGAGCAAATGGAGGTCGTAGATGAAGACCGAGCTACGAAAAATATTGGGGGCATCTGAACTTATGGAAGAAATAACTTTTACCTCTTCCAGGCTAATCCAAATAACATTGGAGCAGCACTCCTTACTAGATATCGTAATCTCGCTGGATCTGAATCTAAATATCAGAATGGAACAAACTATAGCATCTTGGCAGGTTCAGGGGCTTCACTTCCACAGCATCTTGGTGGCTTTGCTATTGATGGTAACTTCCTCGGTCGAGGTGGCGGGAAACTTTATCAATTCCGAAGAGCCTCAAATATCTGAACAGCATTGGATTACCGCGAGGTTCCGATCATTGGAGGAGATAAGGTTGGTTCGCTCTACTCAGATAACGTAAAAATTATTGCTACGGATAGTTCTCCATTTATTTACCTCTTTGACAAAGATAATCAAACCTTTACCGTCTATGAATCAAGTCCTGTAAAGACGAACGACAACTACAAGACGAACTTCAGACTTTATTATCTTTTTAGATTTAAGTTTAACTTGGCTGGGACAGAGAACCGTATTTTGGATGTAGCTGTTCCAGAAAGTACTGCAGATAGACCAGAACTCCATCTCCTCAGTAATGAAGGTGTAAATAAAATCAATCTCTACGATTTCATTGATAGTATCAAAAATAATAAGAATCTGAAATCTGTGAACAATGCGTCATAACTCTTTTAGTTTCTTACCTTGAATATCCTCATGAAAGGCATCAAATTCGGAGTCATCCTTCTGCTTTTGCTCCTTATTGGAGGAGGAATCTGCTGTAAACGAAGTGAACTCAAAGAATTTTTTATCGTGCATGCTCCGACACATGAGGAAGAAGTTCTTTCTGCCAGAGGGATCGAAACTCCTCAGCAAATCTTTTCTGGGATCTCAATTAAAGCTATGGATGATCTTGGACTCACTGGCGAGGAAGCTGTGGATGCTTACTTGATGGCGAATCTCCCGAGATGCCACTTCCCAGAGCTCAAAAAAAACAGTAAACCGATTGTCATCGACGAAGTAGTAAAGAACTGCAAGAAGATACAAAAGGTTGGTAGAGTCCCTGATGGATTAGGAGGGGCTTGTTATCCCTCCTTCGCTTATGATCGTGTCGGAGACAGGGTATTGGAGATGAACGCAAGAGAATGCATCTATGGCATCAATCCTGCTGAATACCTCGCTCGCGGAGTCAATCCTAGCTACCTTTGCTTAGACCCGCAAACTCCTGAGAGAGATTGGGATGAGATTTGAGAACTCGCCCCTGAGGAGAGAGACGAACCAGATGGGAAGCCCTGAAAAGCTCCGTATTCTCTACTAGCACTCCAAAAAGCTTTGAAATCAGGACTCCTAAGCAAGGCTGTTCCTCCTTACTATAATACCCCTGGACTTTTTTGTGCAGAGAAGGATCCTGATTGCGAAACGACCTACGATATTGGCTCTCGAAGCGATACCCAAGGGAATTACTTCGTGGCTGGAGAGAGGACAGACCTGCCTGACAAGAAACTCTATGCTATCAATAATCAGCTTTACCTTCCTTGAGAAGGTTCTATAGGATTTAGCTCTGAGAATGGAGAACTTAGCGGGCAGCGAACCACTCCTTGGTTCTTTGGAGGAATAAAAGAAGGAAACCTGATCGTAAAGAGAATGATCGATATGACGCCTAATGGGAAACTTATCCCTGGCGGTCGCATGGAAGCAAACTATAGTCTAGAAACTTGCGAGATTGATCTTTAAAAATGCATTATCCGTATTTCCCCCGTCAAATAAAAAAAAGACCCTGAACAGCCTTCAGGATTTTTTTTAATTTTTCAGGAGTCCATAAACGCTCCATATTCTGCATTATTGAGTTTTGTCAATGCAATTGCCTTGATTTTTGTAATTGAAGTCATCTTTTCATTCAATAAACAAATAAAAAAGCTATAAATTTTGAAGTTTCCGCTTTGTAATAGGGAAGCGGAGGGGTCCGCTTGTCCCAAAACGTCGTTTTTCTTGAAGCGGAGGGGTCCGCTCGTCCTAAAACGTCATTTTTCTTCAAGCAATAATATCCACCCGTCCTTGCGAGCGACCGAAGGGAGCGTGGCAATCCAGATTGTTTTTCTGGTAGCTTACTTCATGCATTCTTCCCACAAGGAGAGCTCCTGCTTGTCATTTTCTTTCCTCTATACAAATAATCCTAGTTTTTCTTAAAGGTAAAACGACCACACCCTCCTAAAGAGATGGAAGCCTCTCCAAAACAACATTCAGGCTCTGTAGGCTTACTTCTTATTAAAAGGGATTCTCCGGTTCCTGCGTAGAAACTCTGAAAGCTTTTGCAAGTAGGGAAGAACAAATGGCTGCAAGCAAAAAAAGGGAACAAAATTTTTCATTTAAAAAATGAGCAAAGATATTTAGCAAGAAAGCAAAGAGAAGAGCAAACCGAGAAAACAAAGGAAATTATGAAAGAGGAAATCTCTTGCATTTTGGAGGGGAAAAAACTACACTTCTTGGGTCAGATTTTTTTATCTTTTTTATAAAGAGATGTTTGAAGGAGCATCGGTATTGGTTAAATTAAGCCAAATCCTCTTTTATGCGCTAGTGAGTTTTGGGATTTCACTCTTGCTCTATCCTGCGTATATCAGGTTGCTTCAAAGAGTAAAAGCAGGAAAAACGATCCGCGAAAATGCGGCAACAGGAGAAAAGTCTGAGATCTTCAGCAAAATGCATGCCCACAAACAAGGCACACCAACCATGGGAGGAGGAATGTTTTTGATCGTGATGTTGGTGATGATTCTGGGGTCGTTTTTGCTCCAACATTTTGGATGGATCGGCAACTCGCTCTGGAATGCAAAAGAATCCTATATCATTTTGTTTGGATTTTTTAGTATGGGATTGATTGGGCTGGTTGACGACTTTCTCAACATCAAGAATTATGGGAAAGTGAAGGGATTGAGTGCGAGAGCGAAGTTGATTGGAATGGTGATTTTCTCGGCTTGGATTTCCTATTGGTTTTATGTGAAGCTCGGGATTGATTATATCAATCTTTGGCCAATCGCATGAAAGATTGAAGTCTGAATTTTGTTTCCTATTTTGACTTTCATTGCGACGATCACGATCGTGAATGCAATCAACATTACTGACGGATTAGATGGCTTGGCTGGCGGCACGATGGCGATTATTTTGGTGAGTCTCGCAATTGTGACCTTTTTGAATGGAACCTATATTGCGACGACAGTAGTTTGCATTCTTGTTGCGGTTTTGGTTGCGTTTTTGTTTTTTAATATCAACCCGGCGAAAGTTTTTATGGGGGATAGTGGTGCGTTTGCACTCGGAGGGCTACTTGCCTCTCTGATCTATCTGCTCAATATGAGATTCTGAATTTTGATTCCTTTTTTGATTATTTTTGGGATTTTTATTGTCGAGCTTTGTTCGAGTTTTCTCCAGATTTTTTGGAAAAAGGTTTTTAAGAAAAAACTCTTTACGGTAGCACCGTTCCATCATCTTTTGGAATACAAGGGAATGCATGAAACAACGATCGTTATGAAGACTCGACTGGTGCAAGCAATTCTCGCAATCGCTGGAGTATTATTGATTTTTGTAGAATCATCCTTGATGAAATAAGAAATTATTCGCTTTTTTTAATATCGGTGGTAATAAAAGATTTTTCATAAGGGGAAGCTAAGATTTGGACGCCGACGTGCTGGTTTCCGACGAACATCAAACCTTCTCCGACTCCGACAGAAACTAGTCTTTGCTGTTCTGCTTCCGAAAGTCCGAGGAGCTGATTGAGTGATTTGATAGAAGTAACGGATTGTTTGAGGAGGATTTGCATTCCGGAGTTGGAAACGATTGGTTTTCCATACGGACTACGGATGAAGTCCTCAATATCCTGCGAGATGGTCGTGATTCCGAGTCAGTATTTTCTGGCTCTTTTGATAAGACCGAAGAGGAAGTTTGCAGAAATATCGTTTTGAAGCATAATCCACGCTTCATCACAGACGAGAAGTCTCTGCTTTTTGACCGATCTCACCTTGGACCAAATAAAATTAAGCACATTAAACATCGCAGGGGTTTTCAGAGCTTCTTCCACATCACGGATACTAAAGACCGTAAGCGCATTATTGATGTCAATATTCGTGTAATTGTTGAAGAGTTTTCCGAAGGTTCCAGTTACATATTTACTGAGCTTAAGTGCGATTTGCTCTCCTCCCTGCATTCCATTCAAGACATTCATCAGATCTTCCATCAGCGGAGGAAGTTTCCCCGTGTAGTCGTCCATTTCAAACGTGAAGCCTTTCAAGGCGTAAGTATTTTGGAGCGCTTTATCAAGGAGAGCTTCCTCTTCTGGACTAAGTTTTCAAACCAAAATCTGAATCAAGCCCACGAGATTCATAATCTGCGAACGAAGCAAATCTCCCTTTCCATACTCCACATCTTCAATCATCGGTGGCATATCAAAAGGATTGAGAAACTGCTGAGAAGAAGTTGCAATATTCACATAGGTTCCGCCTACTCTTTCACAAAGCGCTTGATATTCGTTTTCAGGATCAATCACAATAATATCTACTCCATTGAGCAAATACCTGAGAATCTCTAGCTTCACCGTAAAAGATTTACCGGCACCAGAAGTCGCCAAAATCACACTATTCATATTTGGCAGTTTGGAGTTGAAACGGTCAAAAATCACAAGTCCTCCGGTATGAGCATTGACTCCGTACAGGATTCAGGTTTTGGTAATAAGATCACTAGAAATAAAAGGAAAGCTGGCTGCCAACGAAGAAGTCACCGCACTACGAACGATTCCCATATCATCAAGGCAGATCGGTAAATTCGCTGTAAATCCATTATCCATCCTATGAATCGCGTTCTTGACTCTGATTCCATAACCGGAGATTTTCTGCTCAAATTTTTTCCCGGTTTCTCTGAGTTTTTCCTCGTTGTGATCGTAAATGGTGGTATAATTTCAGAGTTCAAAGTATCTTTCCTCTTTGGTCGTCAGTTTTTCTCTGATCATCTCTACATCACGGTATTGCTGTTCAATTTCCTTGTCAATCGTAATTCCCTTCTGCATCGCATCAGAAAGCTCGGCTTTGAGCTGGGTGGATTTTTGCTTAAGCATAGACTGGATCGCCGAATCATCCTCAGGATAGAGGTAGAAACTCATATCTCGCTTGGCATGCATGCTCATAATATCCCTCGTCCAGAGTGCATCAATATAACTCGGATATTCCTGAGTATAATAGCATTTTCCGAGGATTCCAGATACATTAAACTGCTGCATCGTAAACTCTCGGTAGGACGGTGCAATATGTGATTTATAATCAGAAAGTAAAGCTGAATAACGATTATCAACCTCTTTGATATATTGTCTGAGCTCGGAAGTCAGCTTTTTGTCGCTATATTCCGCATCTGGAAGCTGTAAAAGTTGCTTCACCTGATCTGCGAGAGCGAGCTGTTTGTCGGTTTGAGGCTGTTCTTCGGTTGGGAGTTCAGGTTTTTTGGCTGTTTCTGAAGCTTCTCAAGGCTTTTTTGGAGCTGGAGCAAGTCCGAGTTTCTGAAGTAAGGAATCCATCCATGCCATCGAGTTTTTTTTCTCGGGTTTTGGTGCTTCTCCTGAAGATTGCGGTGTGTTTTTTGGGAGAACTACAAAACCTCCATTCGGAACTATGGTAGTGTAATTAGTATCATAGACAATGTTTTGTAGCTTCTCAAGCATTAATTCCTGGATTATCTTTTAATGGTAAAATTCATCTCTAAGTATAAGCAAAAAAGCTCATTTTTCAAAAACTGGGGTAATTTCTTGTGGAAAAGCTACTTGACTAAATGCAAAAAAACAAAAAAGACGAGGAATTGTCTCTCGCCCTTTACGGAATGAAAAGTCTGATTTTAGATCTTATTCTTCCAATAAATCATGAACTCCTTTGAGGAAATGATCATCTACGTGGGTATAGATCTGAGTTGTTTGAATAGAAGAATGCCCAAGCAGTGTTTGAACGGATCTAATATCGGCTCCTTTCTTGAGCAACGTAGTGGCAAAAGAATGTCTCAGCGTATGAGGCGTGACTTTCTTTTCGATTCCAGAAAGTTTGGCATAATTTTTGACTAATGCCTCTACTGCATTTCTGGAAAGCTGTTTTCCATAGCTATTTTTGGAAAGGCTGATAATGAGTGGTTCATAGGTATCAGTTCTCAAGTTTAAGTAGGTATATAGTTTTTCCATCGCAAAAGAGGTTAAAAAAACAGACCTTACCTTGGAACCTTTTCCTCTGATTGCGAATTGCTTTTCTCAGAGAATGAGCTGACTCTTTTTGAGGCTTATTAATTCTGAGACTCTCAATCCTGTCCCATAAAGAATATGGAGAATCAATTCATCTCTTGCCTTTTGCAGTTCTGAAGTCGCGAAATGAATTGGTGCGCTCAGAATTTTATCTATTTCATTTTCCAGTAAAAAACTTACCTCTCTCTGTGGTATTTTTGAAAGTTCTATTTTGTCTGGACTGAGGCAATCAATATCATTTTTGAGGAGAAACCTTAAGAACGATCTCAAGGCAATGATATGATAATTAACGGTCTTTTTTGCAAGTCCTAAATTATTAAGTGCCATTCTAAACTGTAAAATGTCTATCGCTTTTATCTTGTCTACCTCAGGATCTCAGAGAAATTCAACAGCTCTTTGGAGCCATAAGCGGTAATTCACTATTGTTTTTGGGCTTACATTCTTAGTATGTTCCAGATAATCAAGAAATGTCGTCAGTAACGCAGACATCTTGTATAGTCCTTTTTTTGGAGTTTCAGAAAAAGAAGGTTTTGGCCCCTCTTTTTTCTGATGATATCCAGTCCATGAAGAACTTGTGATAAAGTTTTTGTTAGCCATTTGTTGCTTTAGTTAAATAGTCATCAAGCTGGGATTTTAATTCTGACCATGTAGATATATCAATATTTTCACCATTTGCAAGCCTATCCAAGATTTTTTCAGACTCTTTTTCTACTGCTAATCCATACTTGATCATTGTTCTTTTATTTTGAGCTTCTCACATCTCCCTTACTTCCTTTCCTTTTCTCTGAAATTCCATTACCTCTTGCTTAAGAATGTCATTATTATTAACAGGACCTACGAGATTCTCTATTGCTCCAAGTCCATCTGCATTGTTTACTCCTGAAGCAGCTGCGTTCCCCGTTGAAGGATTTGTTGTGGTAGATGGAGTATTTGTTTGAGAGAGATCAATAGTTTGGATAGCGTCTTCATTATCATCGTCAAAGGTTCCGGAAACAGAAACAGGATCTGTTTGCTGAATCTCTCCATCATCTAAAGTCTGCATTTCTCCTGTTAAAAGAGGATTTCCTGGTGCTGCGTTGTGTTCCTCTCCTGTTCATAAACTCATCTCTCCTTGTGGAGCGCTGAGAGAAAACAGGTCTGGATATCTAATATAAGCAATTACCCCTGTAGCGATTACTATTACGGCTCCAAATACTACTCCAAGCATTTTCTTTAGAGGTAAGTTGGTAGTTGTTAGCTTTGGAGTTTGCGATGAAGCTGTGAGAGGTGCTATCTGGGGAGGAAGCTGGGCAGATAACATATCCAGATTTAGTGATTGAGCTTGAGGATCAGATGCTAGACTTTCCTGAGAAGGAGTCTGTAAGGTTTCATTCAGTGCTCCAGTTTCTGACATAGGAGAAAGTGTAGGTGCCTGAGTAGGTTGCTGCTGCGCCTGAAGAGTTTCTTTCATTGCGGTAAAAGGATCAACAGCAGTATCTGAAGTAGAATTAGAAACATCTTGAGGCGATTGGGCTAGCATCTCATCAAGATTAAGCAAATTTCCATCTCCTGCTGGTACAGATAGGGATGAAGGCATTTCTGTTGGCTGCTCCTGCTGGATTGGCTGATTTTCAGCGGATTGGCCGCTCGTCATAAATGGTTCAGAAGGTTGAGTGTTAGCTTCACTTTCAGGAGATGCGTCCATAAACATCTGTGAAGGAGCGTCTTGATTAACTTCTTCTGGGACACTTTCATTTGCCGTAGGTAAGGCATCTTCAAGAAATACTGCCTCATCTTTAGCTTCTGATATTTCATTTGGTGAGAGGGTTTCTGTTGAAGGAATTGCATCAGTAAGAGAAATGTTTTCTTCTCCTTGCAAATCTGACTGGAAAGAGCTTTCTTGATCTACCGGAGCAACATCCTGTCACGTAGCGGAAAACATACTTCCAGCTTCTTCTGGAGAAGTCTGCTCTTGTGCGGTTGTTTGTGATAGGTCATCTTGTAAAAATTCATTCGTAGGGAAATCTGACTCTGATGAAGGCACTGTTTCTTCCTGTAAAGGCTCTTCTGACATCATCAATGGAACCTCTTGGTCAGAAGGAGCTTCTTCGAGTAAAAAGTCAGTATTATTTTCTGGTGATACCATCTCACTACTTTCTGGATTTTGTTGCTGATTTTCAACTCCTACAAAAGGAGTAGATTCTTCATTTAAAAAACCATCGCTAACCTCCTGACTTGGCATAGGGTCTGCAGGAGGTGTTTCGTCTTGGGCGTTAAGGCTCGGCATCTCGGAAACATTCGTATTTTGAAGAAGTTCCGTGGATGACTGATCCTGTGCACTCTCTACAACTGGAGTTGGTGTCTGCTCTGCATAGGTATCAGGTAGATCTAAAGAAAAATCAAAAGACTGATCTCATCCTACATTTTCAGAAGAAAACTGCGTAGAAGGAGCTTCCTGTGGTATTTCTTGCGTTATACCAAGATCAAAATCGTCGTTTTGAACTGGAGGTTGAGCTACTGCTGGGGATTGTGGAGTCATGGTTGAATCCGTAGTTGGCATTTTTATACACTGAATGAATAAAATTTTCATTTCCCATTATAGCGACGAAACAAAAAAAATGCAAATTTTTGCACTTTTTATTATGATGATCAAGAAAGAGAGGAATCGAACCTCCACTTTCAGCTTCGGAGACTGACGTGCTACCACTACACCACTTTCTTTTGAGTAGCAGGATTGTAAAGATCTTCCTTAATTTTTCAAGGTTGAAAAGCCCAAAGCTCTCATGATTTTTTTCTTCGTTGTATTGCTTTGAAGAGAGGCTTTTCGTAGGAGCAGAGATCTGATTTTTTCTCTTTGAATTAAGATAGAGTCGTTTATCCTGTATGTCTTGCGAGAACAAGCCCCCAAATATGCAGATCTGGTCTAGAGGATTTTGCTCTTTTTGCAAGTTCATTGAGCGTAGCTCAGGTCGTCGTCACATCATCTACGATGAGCAAGGTTGCATTTTGAGGGAGCAAGCTTATTGCAGAAGGAAGGAAAACGAATGCAGCATTCAAATTTTTCAATCTCTCTGATCTCGTAAGTTTCACCTGAGAGGTCGTAGCCTTTACCTTTTTGAAGCAAGGAACTCGAGGCATCTCAAGCTGAGTTGCTAGTTCTTTTGCTAGGAGTTCAGATTGATTATATCCCTTCTGAAAATATTTACGATACCGATGCGAAGGAACAAAAGAGATAAAAAGCTGATCCTTTTGCATGGCAAGGGTAAGATCTTGATTAAGCTGCGTCAATAATGCTCACCTTTCTGCCAAAAATCCAGCAATATCGCTCTTGTGATGAAACTTGAGCTTAAGGATGAGTTTTTTGAGGAGAGTCTTGTAGGAAAATCCGACTACAAGCCCATCAAGGAAATTTGACCCTTCAAATCTGCAAGAGCTACAGGTTTTAAAGTTTTTACTCTCTTTATGACAAAAAGGACATAACTCTGGATGTGGAAAAAGTTCCTTTTTACACTCAAAGCAGAGATATGCTCACGGCTTATCGCATCCGTAACAGGAACGAGGGAAGAGGTATTCTAGCCATTTATGCATTGAAACACGGAATGGCGAATAAAAGCTAGACTGTTCATGCATCTTTTTTGAAGCTAAAGCGGAGGTTGATTTGCAAAGTAGCAACTAAACTAATAATCGCTGATATACAGCCAATCATAGGAATTCGTGCAACATAAGGAACTACCATCGGTTGCATAAAAATTCGTTCGTAGAAATCGCCTTTGAAATATGCAAGGGAAAAATAGAAGCAGGAAATCAGATTTCAAAAACTTAAAACACATCGAAGCAAAGTCTGGAGTTTTTTTTCAAAAATTTCTGAAAAGATTTTGATGCTTAAGTGTCCGTATTGGATAAAGAATCGTAGCCCTGCCACAAAAAATATGAGGAGTATGGTCGGCTGAGCATTGATGATAAATTCTGCAATTTTTGCACAGCGGAGTCCTAGAAAGGTCTCATCTGGAATACCTTGCAAAAGTGATGCGCCCTGTAAAATACCTGTTCCCAAAGCAAGAGATCAAGCCAGAAGCAGATAACTTCAAAATGACATCCTAACCAGCTTTTCGACCTTTAGCAACGAAAGTAACGCGACAACTAAGATAAGTGAGAGCACAAGGCTTCGTTCTGTAGACATCACAATGATCAATCTGAATAAAAACTATTGCATCTTTGGGAAAGATAACTACATATAGAGGCATATTATGTTTTACCTGATGAATGTCAATTTTATTTTTGGGAAGAGAGGCATGAAAAAGAAAAAATACTGATTTACAATGCTAGAGGTTATCCTTGTTCTGATGATCATATCTACCGCATTGATAAGTGTAATTACTGGAGTTGCGAGAACAGTAAACTACGTCTCAGAGATGAAGCAAAGAACCCTAGCACTTAACCTTGCGAAAGAAGGAATCGAAACGGTATATAATATTAGAGATACTAATTGGAGAAGATGGTCTGCGACCAAGGATAAATGCCGACTTAAGGTTACCCCACTTGATGGATGAAATGAAGGCTGTGAAGACGATCCATGGATTACCGCTCATACTAATTGGGTTATTGAAGAGCAGTTAGCTCAAAAAGACCGCTACTTTTCGCTTACTTGATGAGACGATGATCGGCCTCTTTTTGGACAGAATGTAAAGCCAAGTGATGTCTATACTTTACTAGATCAAAAAGATCAGACTCCGCAAGGGACGCTCTATAGACTCTACCTCCAGGGAGGGAAATGGATTCCACACAATGTTTTTGAAAACCTTAATGCGGCGGAAAAGGACAGAGTAAAATCAAACAATAAACAAGGGAAGTTTTATAGAGCAATTAGAGTGCTAGGACTTTATGAGAAGAATAGCAATCTTCCTGATGAAAAAGTGAACAATGGGAATTGTCCAAATTGATTTGACAATGCATGTGGTTCGAGTTCTGCGAAAGAACTTCGTTTTTGTTCGACGGTGTTTTATACCCATCCTTATCAAGGCACGGTTAATATCTGCGCGATTATGACAAATTTTTTGGAATAATAGTTTAAGTCTGATTTTTTTGGGGAGTTTTTATTTTTCCGCTATATAAGCGACTTTCTTGGAAAAGAGGGTTGCTTTTTTATTTTTTATTATCATTCTCCGCCACTGAAATATATCAAATACAAAAAAACACTACCAGGAAGCGAAGCCATGGCTTCCACTACCAGATAGTGCTTATTTGCAATTTCGATTACATTTCTACCTCAACTCCCATATTTCTTGCGGTTCCTGCAATAATTTTGTAGGCTTCCTCTGGAGTATTTGCGTTTAGATCTGCCTTCTTGAGTTCATAAATTGCTTCAAGATCTGCTTTGGTAAGCTTTCCGATCTTTTTCTTGTTTGGCTCTCCAGATCCAAGATTTTGATTGATTCTTCGTTTGATGAGTCCTGAAGTGATTGGAGCACCGATCTCCATCTTGAATGTTCTATCAACATAAACAGTCAGCTTTACTTTAATTTTTACTTCAATTCCTCAAAACTGCTTCATCAAATCTGCAGTTTTTTCGTTAAATTCTTTGGTAAACTGCCCGATATTTACTCCATTTGCACCAAGCATAGGTCCAAGCGGAGGTGTAGGTGTTGCCTTTCCAGCAGGAACTTCGATGTTCAAGATTTTTTGTACTTTTGCCATAGTTACTTCAATTTAAGAGATAAATAATTTCTTGAGATATGGTTTCTCGTGATGATATTTTTTATAGTCCATCTGAATGAGCCAATACTGATCATCAACGGTAAGTAGCATAATTACTATTTTATTTTTGTATTCTGGGATATTGACAAAGCTAATACTTTCTCCAAATAGTTTATTCTTGAGTATCTCCGCTTCGGAGCTCATCGTATAAATATTTCCATCCAGATCCTGTACGGTTTGTTTGAGATCCGGTTTCTTTGAGAGTTTTGCATAATAAAAGCCTTTGTTATCTTTGAGAATATATTCAGGTTTGAGTCCAAGAAGCTCAACACTTTCTACCGCTCCGAAATAGAAATCAGTACCGGAAAGTATTGATGGACCTTCCCCTTCAAATGAGAGATGATTCGCAGGTGAAGCTGTCTGTGTTCATCCCGTTTCCACTTGTTGGGCATCACTTCAAGTGGAGCTATCTGAGAAAGAATCTCATTCTAATGTTCAAGTCGCTTGCGTATCGCCTGATAGCAAATCAATATGTAAATCTTCCTGTCCACCGCTTTCCTGTTTGCTCTCTTGAGAAGCAGAAGTTGCGGAGGCTGCACGCAAGATCTGGATCAGCTTTTCTCCTTTTATCTGATAGAATAATGAATACACCACAATTAAACTAAAGAAAAGCACTACCATTGATAATGTTACGAGCTTTTTTTCTTTTTCCATCGTTCTGGTTTAAGAACTAAAGCCTAGTTTACTAGCTCTACCTGGTCGATTGAGATCGCTACAGGAGTGAGTCTTCAGAGCATCTCTATATTTACTACAACCATTCCTTTTTCAGGATCAAGCTCTTTGATATTTCCTTTCATTCCCTTGAAATCACCATGCTTTAGGAGGACTACATCCCCGATTTTGTAAGGGATATTTAGCTCAGATCTTTCCTGAGACTGAGTAATCTGTCTCATCATATCTTGATACTCCTGTTCTGAAAGAGGAATTGGCTTCGTTTCAGCCCCTACGATAAGTCTTACTCCTGGAGTATTTCTCACTACATACCAGATTTTATCGTTCATCCTTGATTTGAAGAACACATATCCAGGGTAAAGTTTTTTCTGCCTTATTACTTTTTCTCATTTTTTGATTGAAGTCTCGTTGATCATAGGGCAGAGATAGTCGATCACATCCTCTGACAAGCCCTGTTTATTTATCCTCTCCTGAAGGTTTTCGATCACTAGAGCTTCTTGTCCAGATGTTACACTCAAGACGTATCGTCTAAAACTCCTATCTTCGATCTGTTTTTTGATTTCATTGGTATTTGCTTCTTTTTGCATATTTACTCAAGGGAAGAGATAAAAAAAGCTGATGTTATTCTGAAAGCGAACTTTCAGCTCCTGATTCAGTCATGTCTATATCTTGTATGGTGCCTTGAGCATCCGCCTGTGAGGCTTCTGCACTGTCTAAAGAAAGCCCAGAAATATTAAGTGCTGGAAGAATCGGATTTTTTGCTTCCCCTGAAGCTACAGCTCCAGAAGTGGTTGTCTGTCCATCTGTCATTGCTGCATAAAAAGCCTTGTATCCTCCTGTAAAAAGCGTATCTGCGAAAATAAAGTACGCTCCTGCAACAATTACTAGACCAAAAATAGCGAACGTTAACCCTACGATCTGCTTAACTGTTGGGAATTTAAGCTTTTTGACGGTATCTAAACTATCATAAATAAAATCTAACATTTCTCCAATAGCAAAAGATAAAACTGAAAAGAATAATAGCAATTTGCCGTGTAAATGCAAGCTAAAGCAAGTAAAGATTTTTTATGTTTTTTTGTCCATATATTCTTTTACGACTTCACTCATTGCTTCAACAAGGACATTGAATCTCTCATTATCCTCTGAAAGATCTTGTTCGATCTGTTTTTTCTGTCTGGTTAGAAAAGATTCTTTGAGCATCATCAGCTTTCAAAGCTGTTTTTTTGGAGCATCGATATCGGTAAAAGGGAACTCGGTATCAAATTCTAATTTTATTAACAGGTCGCCTTTGGCAAAAAGTTTGTCTGAGATCCCGTTCTGACTTCGAGAAATCGTTGTGATTTTATTTCGAGAGAAAACCTCAGTTTTGTATTCCAAAAGTCCTTCCCAGAGAAAAAGAGTAAGAGAATCTTTTGAGAGAATTAGACAATCAAGATAACTATTCAGAAAATCAATAGTCCACTTAGCAAAGAGAGCAAGTCCTATCACTCAAAAAATCCGCTTTCGATATTCTGCAGGAGTTGTTTTATGCAGCAGAGCAAAAATTACGTATAAAACAAAAAGCAGAGCAATCATCACAATTGCTCTCTTTACATGCACTACGGAACTATGCCCAATTACTCGTTGGATGTCTTTGGTATCTACATATCTTTTGATGAGTACATTTCTCAGCATCAGCAGAAATTCCTAATATAACCTAAAAAACATCAAAATATAAGACCGACTATTTATTATTTTTAGCTGCCTCAATTCTCTTCTGCTTTTCAAGGTATTTTTCCATCATTCCTTTAACTGGCTTTGTCACTACTCTATCCTTATTGAATACTGGATGACACTGATAGCAAGTTTCCACCTTAATTGGTCCTTCAACAGTGGTATTTACTGTAAATGATGCTCCGCAAATGCAATTTACTTCAACGTCTTTGAAGTATTTTCCGTGAATCCCTTTTTTCATCCCACTCTTGTAAACAAATAAATGTTTCCGAACAGGATGAACTATACAGATTTCACTTTCATTATCAAGTTATTTCATAGAGAAAAAATCAGATTTTTTTCTTTGTTCACTCTACAACGAGAGATTATCAAAAAAATGGCGGTATTTTGCGATCAACTCTGGCAAGCACGTAATTTCATCATCAATTGCTCTCTTATTTCGATGCTGCTTCCCACATTTTACGCAGGAAAACAGGATTCTGATTTCACTATTTTTCATTTCATAACTCGTCGGATACATTGCTCATTGACACTCACTTGCTCTATCTCAAGGAATTGGACCATCTACATGTTTTGAAGCAAAACAAAAAGGGCAATGATTACGGCAGGTTTTCTGTGCTTTGGGAATTTCTTTCCCACAATTGAGACAAGGAAACGTTTCGTTAATGGTTTTCATAAAAATAATCCGCAAATAGAGATAAAACTCAGCTTCATACTAAGGACTTCATTTCCTCTGGAGTAAAGAAACAAAAATCCTGACATTCCTCACTCGTAATGAAGTTAAAATTCAGGATTTTTACCTCATAAAAAAGATGCAAGCACCAATTTCCCCTTTCTTCTCCCAAGGAAAAACAAGTTTAATAAAAATAAATATGCCAGCTATATGTATACAAAGAAAAAGAGAAATCAAGCACTTTATCATCCATAACGAGGATCTCTAAAAGCTATGGAGTCGATGATACTGGAAAGAAGGGAGCGAGATCCGCATGCTTGTCAGTAATTTTCATAAATGAATAGTAAGTTCCCATCTCATCCACAGCCTTCATAAATCTCTCTACAGGTACTCTATGATAAAGCAAAAGTGGAGGACCTGGATCAAAAATCACAATATGATCATCTTCAATTTTTGATGCTAAGATAACGTGTCCTCATGGTAAATCTTCATAAGGTTGCTCATTATGTAAAGTATACCTATCCAAACCAATCAGAAACAAACACCCTTCTCTTTGATTCTCCTTAATTACTTGAATAATATCCACATCATAATGAGCTATAATTGACTGGCTATCTACTATTTTTTGATCAAATTTAATATCTCAAAGCCTATTAATCGAAGTACCAGAATCTCGATAATACCCTTGCTCATCTGTTTTGATATATTTATGATATTGAGATGGTGTTCGTTCATAGATATATTTCGTAGGATTTTCCAAAGAATCTCTATGCAATTCTTCACTATAGGAGAGATAATAATCAATATTAAATCCCAATTGATCCAATTTACAGGCAATTTCCGGCAAATCTAACATTTCAGTTCCTGTATACAATCCCTCTTTAAAGAGATTCATCATTTGCCAGGTATTCTGTCCATTGAAGCTTGTCGACCAAAAATAATTTAAGACTCCCATACAACACGTCAAAGAACAAAAACCAGGCACCGCCTCCGGTAAAAAATATTCAGGATGTTTTCCATCTCGGAGCATATTTTTTTATCGTAAATAAGTAAAATCGAAGAAAAAATCTGATTTCTTTCTTTTATAAAACATATTTTACCGAAACTTCTTTAGCCCCCATTTTCTGTAAAAAACTAAGATACTCATTCTCTGACGGGGCAACTTCAAAGCTCACAAATTTCGCTCCTTGCTCCTTCAAAAACTGTAGACCATACTCCAAAAGTGCTTGATATTCAGAAGATGCATGCCCTTCTTCTAGAGCGACCATCAAAACTTCAGCTCTTTCCTGATGAAACATAAGAGCGACAAAACCTCAAAACTCTCCCTTCTCTCCCTCTGCAACAAATAGAGGAAACTCTGAACTTTGATCTCCAATTCTCTTACTCCAAACACGCATAAATAAGTCATAATCCGTCTGATACATCGGGCATTTAAGATCGTCATTTAACTTTTGATATTCCTTTACCATCAAAGTGGCAATGAATTGAAGGTGGTTTTGCGTCGCTGGAATGATTTTCATGACTACTATAATAAAAAAATAAAAAGATAGTTATTACGTATTTCTTACCACAATAAAGTCATAATGCTGATCAAGTTCAGTCATTGCGTTTAAGACTCTTTGCTTCGGGATAAAGTCAGGTTTAGGGTAAGGACCTGGATCATAGATTTCAAACCTTCCATCTTTATACCCCTTACACAATACGATATGACCTCCACTAATACCAGATTCATGAGGCTGATCATAAAGCACATATCGATTAAGAGGAAAAATAAAGAGCGTATCTTCGTTTTGGTAGTGTTCCACAATGCTTACAAGATCAGCATTATAGATCTTTTCAATTTCATCAGTTTCTAGAATTTTCTGAGTAATATAGGTATCATAGAGTTCAAAGCTATTATTTCCATTCATATCCACCCAAACCCCATCCTTAATGTATTCTTTGTAGGGATGATTTTCGTATTTTTTGGCAATATATCACACGGGGTCTTCGAGATAAGCAAGATCATTTTCGTAACTTCATGATGATACGTATATTACCTTAAAACCGAGCCTTGAAAGGGCATACGCCATTTCACATTCATCCAGCCCGACTCCAAATTTATATCTTCCCATTTTAGCGATTTTCATCACATCTCGGGTATCTTTCATATTAAACTGAGTATATCGGAAATAATTCAAAATCCCCATGAAACATGCAGTACTACAGTAGCCATAATACGACTCTGGCATAAAGTTAATTTGATACTTTGGATAGTCCATGTTTTTTATTTTAAGAAGTAGAATTGATATCAGACACTGCTATGATACTATAAGTCTTGTTTAAATCATCCATAGCTTTTATGACTTTTTCTATCGGTATTAAATCAGGTTTTTTATAAGGACCTGGATCATAAATTTCAAACATTCAATCTTTGTATCATTTACACAAAACAACATGTCATCAACTAATTCATTCTTCTGGCTCTTGATCATACAAAACATTTCGGTTAACAGAAAGAATAAAAAGAGTATTTTCATTCTGATATTTTTTTATGAAAGACAAAGGTTCGATATTATAAAATTTATCTATTTCTGAACATTCAAGTACTTTCTTAGATATATATGTATCGCAAAAATCAAAAGTATTGTTGCCATTCATATCAACCCGTTTTCAATTTTTAATAAAATTTTGATACTTACTATCTTTATGCTTTTGGGTAATATACGAAATAGGATCGTTGATATATGATATTTGGTCTTCATATCTACCTAGATCTCAAATAGTAACTTTAAATCACAATCTAGATAAAGCATATCACATTTCATAAGTATCTAATCCTACTCAAAATTTATATCTTCCCATTTTAGCGATTTTCATCACATCTCGGGTATCCTTCATATTAAATTGCGTATATCGGAAATAATTCAAAATCCCCATGAAACATGCAGTACTACAGTAGCCATAATAGGACTCTGGCATAAAGTTGATTTGATACTTTGGATAGTCCATACTTTATCAAACAATAAAATAAAAGGAGAGTTATATCTTTTGGTTCGCATTTCTTTTCATATATACATTATGATCTTCACATACCTATTTTATTACAATAGTTATTTTATAAGTTGCGAAAAGGTTAGGTTTTTTATGATAACCTGGATTGCGTGTATCAAATTCCATCTTTATGCCCTTTAAGCAAATTCATCTATTATTTATTTTTTATGGTAAAAAAATTATTAACGCTTAGCTGAAAGACAATGTAGGATAATCCTTGATATTTATTCCATATCTTTTATATACCTCTGTGGTATTTTCTGAGGTAACTTCTCCACTTACTGACAGCTTTCTTAGAACTTCGACAACAGCCTCTTTATTCGGTCATTCAATCTCTAAATATGCAGGAATAAGTGGTCGACTATCAACCTCCAAATTACAATCTCCCAGAATAAAAGATTCACGTACATTCTCTTGATAACTTTTTGCACGATAATCTAATTGCTCAAGAAGAGTGTTCATCTGGTCAAAATCCTCTACTCCAACTTCTCGTTCTTGTACTCCGTCAATCGCTTTTCTATCAACAATATGTTTTATTGTTAGGGTTGTGATCTTCCCATCTGTTCTAAGCCTCATCCGTTTTTCTGCTTGGGGTGGATTCATATCATATACATATCTACGAAAGTTTTTTTTCCCAACAAACACTGCTCCAAGTTCCTTTAACTGCTGCTTCACAGCCTCTACATCAATATTTAAAACTTTAGTTTCGTACTCTACAGCCATTATTTCTTTTCTTAAAAAATAAAAAAATCAGATTAAATTATAATATCCGTTATATATCTAGTTTTTTGATTCTATATAATCATAAAGCAATTGCTCTTTTTAAATCGTCTCCAACTAATCAATCTAATGGTATATCAAAATTCTTCATGGAGAGACATAGGTTCAATACCTCATCTGGTGATATTCTCAGGTATGAGGCTTCATGACCACATACATCTTTATGAGTACAATTTGAACATTTTTCTTACGTTCTATCTCATTTAAGAGATTGAACTAAATCTCTATGCATTGTAGATTGAAGTTATTGAATATGTAAACTCTCCAATTCATTTTTTGTTACATACAGCCTACCAGAACTTAATTCATCGCATTCTATTCTACCTGAGTTAAAACCATCTCTTGCGAGGTTGTATCTTTTTTAATGTGTTTTTCAAGGAGAATCAAATCAATCTTTTACAACTAATCAAAAAAGTTTCCTCTTTTAGAGACAATTCCCATGGGGTAGCTGGGGATCGAACCCAGGACCACTCCCGCTTGAGCGGGATTGCTCTACAACCACCTCTCTACATGTCCGCTTCTTTTTATTTTTCTTTCTAAAATAATAGCTTCTGTTTTAGAAGCGCAAGGGGTTACTTTTACCAACTCCCATGCTCCTATTTCTCTAGTTGTTTTTGTTTGTCCTCTCTTGTGTTCCTCTAGCCTTCCCTGCAAATTTCCAGTATATCCTACATAATACCTTTTCCCTTTTAAGATATAGACTACAAACTCCATCTCTTCAAAAAATATTTAAATCAAAAAAGTTTCCTTTTTCAGAGATAATTCCCATGGGGTAGCTGGGGATCGAACCCAGGACCACTCCCGCTTGAGCGGGATTGCTCTACAACCACCTCTCTACATGTCCGCTTCTTTTTATTTTTCTTTCTAAAATAATAGCTTCTGTTTTAGAAGCGCAAGGGGTTACTTTTACCAACTCCCATGCTCCTATTTCTCTAGTTGTTTTTGTTTGTCCTCTCTTGTGTTCCTCTAGCCTTCCCTGCAAATTTCCAGTATATCCTACATAATACCTTTTCCCTTTTAAGATATAGACTACGAACTTCATTTATTCAAAAAATATTTAAATCAAAAAAGTTTCCTTTTTCAGAGATAATTCTCATGGGGTAGCTGGGGATCGAACCCAGGACCACCAGCTTAAAAGGCTGTTGCTCTACCGACTGAGCTACTACCCCACACTGAGAAATATCCACTAAGGAAACTTTTTATCACGAGAGAACTATAGTTTTTTCCCTCACATTTTCAAGTAAATATACAAGAAAACTCCTACATAAATCCTTTAAACTTTTTGTAAAGTCTAGAGTTAATCTTTACCTTAATCTGACTTCCATCATCAAGTTTAATCCATTTATTGATGAGATTTACCTTAAACTGTCTCTTCCCTGCTCTCATAGAGTGAGATCTTGTCTGTCCAGATTGAGATTTTCTCCCTGTAAAATAACATATTCTAGCCATGATTTATACCAATACAATAAAGATAAAATGTTTACTATGCAGCAGCTGGAGTAGCCTCTGGTGTTGCAGCTTCATCTGAAGCTTCTTCTTCATCAGCAAGTTCAACAACGGTCACTACTGGCTGATCTCCATCATCAAGAACTTTTACCTTGCTTGAAACCTTCAAATCCTTTACAAAGATTACGTCGTTTATTGATTCGATCGTTGCTACATCAATCTCAATATTATGAGGAAGGTCTTGAGGGAAGGCTTCTACTTCTACAATATCTTTTACAAGCTGAACCTTTCCTTCATTCAGTTTTTCAACTTTTGATTCACCAACGAGCACTACAGGAACAGACGCAGATACCTTTTCATTCCTATTTACTGCCAAGAAATCTATAGTAAGTACTTCATCAGATACCGGATCCAACTGCATATCTTGCAAAAGCACAAGCTGCTCTATCGCCCCAGTAAGTTCAATAGGCGTAGAGAATCCAGCTTCTCTATATACTCTGATAAAATCATTCTTTAGACAAGTCAAGAGCACAGGATTTTCTAAGCTCTTTCCATATACGATAGCAGGAAGCTGCCCCTCTTTTCTCAAGTTTTTCACTTTTTTCCCAGTTGCAGTACGAACCTGAACAGTAAGTTTCATAATAGCATACAACAAAATTTAAAAAAATTATTTCAACAAATCTGATTCTCTCCTCTCAGCAGCAATCTTACTCTTCATTCTCGCTGCCTTTCCAACTTTTTCTCTAATATAGTAGAGCTTAGACTTTCTTACCTTGTATTCATCAATAAGAATAACTTTCTCAAACTTAGGAAAAGAAAGAGGATAAACCTTTTCAATTGTTTGCCCTGCAGCAAGTCCTCTGATTGTAAAGCTTCCATCAGCTGATCCTGGTTTTCTCACCTTGATGATCAAGCCCTTAAACTTCCAGATTCTCTCTGTAGAACCTTCTCCCACTCTTTCGTGAAGCTCAACTTGCATTCCTGTTTTTACAGGCAAGATTTTATATCCATTCGCATCAGAAAGATACTTTATCATTTCGTGATTCATTATTTAGCATATTTCAAGAATAAAACTACTTGCTTCCTTTGCTATTCATTACTTTAGCTTTCCATTTTCTCTGTTGCTCAAGCATTCTTCTTACATCCATAATCACCACTTTTTCAACTCTTGTAAGTGCTTTATTGAGCTTTCTCTTTGCATTTGTCTTTAATCTTGCCATGAGATTATGATAATTTAGATGATAAATGAGCGAGTCCTTTTGATTCAAGAACTTCTTTGATCTTCTGCTGAGTAATTCCATATTTCTTAAGTTTCGCTTCAGCCTTTACTTTAGCATTCTTGATCCAAGCAGTCAACCCCTCTTTTTCTTCTCTCTTCAAAAGGGTAACATAGCTTTCTACTGCTTTTTCATTTTTACAACCCGTCTTTGGTCTGTGCAAATTCAATGCCTTTAGAAAGTGCTTAATTTTTCTTCCGTTCGTATCTCACATTAGAAACAACAATCAAAAAATAAATTCAGATTTTTCTCTCTTTTTTATAGATTGAAAAACTAGTTAATATATTTAGCCATATAAGCGAATGCCTTATTAGCTTCAGCCATCTTGTGAGCTTCTTCCTTCTTCTTTACCGCAGCTCCTTGATTAGAATATGCAGCGAGGATTTCCTCAGCAAGTCTCTTGTACATCGGCTTTCCACTCTTTCCTCTCGCAGCATCAAGAATCCATTTTGTAGCAAAAAAGAATCTCTTGTCAGCTTTTACCTCTACTGGAACCTGATATACTGCACCTCCAATTCTCTTTGACTTGATCATCGTCACAGGAGAAGCATTTTCAATAGCAGTTCTCACTACTACAGTAGGGTTCATATGTCCGCTTGCTTTAATATCTCTGAGGGTGTCCGCATAAATTTTCTTTGCAACACTTTTCTTCCCATGCTTCATGATGAAGTTGATCAACTTTTCTTCGTAATCAAAGGTGTCCAAAGTCTTATTTTTAATCACTGCCATCTTAGAACTAACAAAAACAAATTAAAATTATTTACCAGCCTTAGGTCTCTTTGCTCCATAGCGAGATCTACTCTGCTTTCTATTTGCTACTGCATTTGCATCGTATTTTCCTCTCACGATATGGTATCTCACACCTGGCAGATCCTTCACCCTTCCTCCTCTTACAAGCACAACACTATGCTCTTGAAGTGAGTGAGCCTCTCCTGGAATATATGCCAATATTTCTGCACCATTAGAAAGCCTTACCTTTGCAAACTTTCTCTGAGCTGAATTTGGTTTCTTTGGTCCCATTACTGATACCTTAAGACATACCCCTCTCTTGAATGGAGCAGGCTGTGCAACCTTTTTGTTTCCTTGAAGCTTGTTTACTCCAAACTGAAGGACAGGTGCCTTTGACTTAAAGGTTTTCTTTACTCTTCCTTTTTTAATAAGTTGATTAATCGTTGGCATTTGATATTTCCAGAAATCTAAAGATAAATAGTAAATGCAATGACAGAATATATACAAAACTCCTCACCAAAATCAAGAGAAAAATCAAAAAATCTGATTTCATTTGTTCGTGTCTAGTATTATTACGAGATTTTTGGATGAGAAAAACGAAAGGAAGCCTGATTTTTGATGATTTAAAATCAGGAAATGAGTTGTAAAAAGCTTTTTAATGAAGTGTTGATTGCGTCTTAATGAGGGAAGAAGAGAAGAAAGCGTTTTAGTGGCGTTTTAGTGGCTTTTTAGTTTGGGAGCACGAGAAAAGAGAGCTGACTTCCTACGACTTTCGGACTACAGTCTCTCCTTGCGAGGAGGTAAAGAAGAATCTAAGACATCGGCTTCCCCCACACAAGGAAACAAGCCCCTCTTCTTCGTGAAACCTCTACAAAAAACGACGTTTTGGGACGAGCGGCCCCCTCCGCTTCAAGAAAAACGACGTTTTGGGACGAGCGGACCCCTCCGCTTCAAGAAAAATGACGTTTTGAGACGAGCGGACCCCTCCGCTTCATGAAAAATGACGATTCCCTGTATTTGGCAGATGATGCTGGGGCTCAATTAAGGATTTTCTCCATTTATTTGCGGAGGTAAACCGTTTGTTGAGGATCAGACTTTAAAACTTTTTTCTTTGGATAAAAAAAAGAGCACCCGTGATGGAGCTCTCTCTTTGCTAAGGATAAAAGGCTAGTCTTCTAAGTTCACATACTCGACCTCAAGATTTGAAGGCTGAGCACTGTCTAGGGCGGCAAGTCCCGTCTTTAAACGGGTCGTCCCACTAGCAGCGAAGGTAATAATATCACAGTTTCCGCTCTTTGCCTTCATCTCCTCGGTAATCACCGCACTGTTGTAGAGCTGGATACAAAAGTCGCCTTCATATTTTTTCTTCCGAACGGCACTCACATAGTCTTGGGCAAGGAATCAATAATCGATCTTATCATAGGCAGCGATTTCTCTTGGATTTGGATTGAGAAGCTCCATTCCTCTCAAGACGAACTTTGCATTTGTGAAGGTAAGTCTGATTCCTGCCTTTTGTGCTGGAGCGACATCAAGATTAACATCATTTCCTAGCGTAGCATCTATTCCTAGCTTCATTCCAGGAGCAAGCGTAAAGGTTTTCTCGTCTCCCTCGTCATCTTGGAAGGGAGCTGGCTTTAGGGCGTGGGCTTCAGGACCATTTTGGTTGTCTAATCCGATGAGTTGAATGAAAAAACTGTCTCTTGCTCCGAAAAGGAATCTTCCCCCATCTTTTAAATCTTGTTTAACCCCGATCGCGTGAAGCACTCCGACTAAAACGAAAAAAGCTCCAACAAGCATCGTGATTCCCAAGAAAAATCGCCTGATTCCTCTCAGAATTTTGGATTTTTTCTGGAGGCAAGCGGTGATGATGCAAACGATGACCGTCATCACAATTGCTCACGCAAATAACTCTAAGAGAAGGCTGATTACCATCTCTCCTGCTGATTGAACTCCCATGTTTATCGTTTAAAAGATAAAAAGCTGACTTGCTTTTAGCTTTTTTGATAGAGAAATCAAGGTTTTTGGAGGCATTCTGATTGCCGAGCTGGAGTCTACTTTTGGAATTTACTCAAATCGTCGTTTTGGGGCAGGCGACGAAAGGCTTTTCACTTCTCATGCTGCGGCAGAGAAGGGGAATTCCTTTGCGGATCGTTGCGGGCGAATGGAATAAAGGCAAGCTCCTCCAGATCAAAGCCCAGTGGTTTTATCGCTATCGTAATGCTGAGGTTTGCAAGGCTCGCTAGAAGGGCAAGAGGGTTCTTTTCAGGTTAGAGAGGGAATAAGAGCCTAATGGCATAATTAGATTTTTTTCTTTTTTGAGCAAGTAAAAAAAAGCTGACCGAAGAGCACGATCAGGATTTTTTTTTGTTGTTTTCAGAAGAAAGGTTATGCCTCTGGATTGTATTTTACTTCTTCTGAGAAAGCAAGGATTGGACAAAAAATAAATGGTAGAAACCAAAGCCCCACAGCAAACATATCAGTTTTCCCGAACTTCTTGGAGATTTTGAACTGAGCAACGATTGCTAAGACTCCAGCCACCGGAGGAAGAAGGAGTCGCCGTGTCCATTCTGGTTTTCCCGCAAGCTTGAACATCAAGTAAATGTTGTAGATCGGGATCAATGCTCCTCGTCCTGGGAGTTCCGCCTTTTCGAAGATTCTCCATCGAGAAACGATCATAAGCGCACCAATCAACACTGTGAAAATAATGAAAACGGTTCCTATTGCTGCTAGTGCACCTCCTATAGCTCCTGCTCCAAGAGATGCCATTTCTGGGTTTTGAGCCATAATTCCTGTTAGATCCATATTTTATAGTATAAAAAGGTAAAAAAATTGATTCCTTCCACTTTTCAGGAGAAAGTAGAGGCTCATTCGTTTTTGAACTATAGTGAATTTTAAACGGAATGCAAGGCTTTTTAGAAGTTAGAAGAAGGAATTACAGCAAGGTAAAGGGTGGCCCTAGGGAAGGAAAAAACGGCTTACCATAGCGATAACATACAAGAGGAGCATTGCTGAGCCTGCCACTCTACCTATTTGTCCCTTCCTTGCACCTCGGGTAGCTCCAAAGATAAAAATGAGGAGCGTGGCAACAATCAAGAGAATAAGGTCATTATTGGTGCCCGGCAAAGCAGGGATTGGAGCGATTACTGCGCTCACCCCAAGGATAAATAAAATATTGAAAACGTTTGAACCAATAACATTTCCGAGTGCGATATCTGCGTTTTTTCTATAAGCGGCGAGGATAGAAGTTGCCAGCTCTGGGAGAGAAGTTCCTACTGCGATGATTGTCAAGCCGATGAGTCTCTCTGAAAGTCAGAAGCTCGTAGCGATAGCAATTGCTCCGTTAACGATTCGTTCTCCACCAAAAAGGAGTCCAACCAACCCGAGTCCGATCCAAAGTAAGGATTTTCGCAAGGGGGAAATGGCGATTGTGCTTTCTGTGTCAGGATTTTCTCTTTTGGCTGTCGCGAAAAGGTAATACACGAAGAACAGCAAGAGTAGGATCAAGATGATTCCTTCACTCCTGGAGACTCGTGAAGTAGCTGCTCCGTCGATCCGAGCGTCATTGATAAAGATCGCAAAGACCAGCGTAGCAGCTAAGCAAATCACCACTTCCCTAGAAAGAAATTTCTTTTGGACAGTGATCGGGGTGATGACTGCAGCGGCTCCGAGGATGAGCAGGATATTAGCGATATTACTTCCCAAGACATTTCCGATGGTGATTCCTGTCGCTCCTTGAAGGCTGGAAGTCACATTAACCACCAGCTCTGGAGCTGAGGTTCCGAATGCAACGATCGTCAATCCGATAACCATATCAGAGATACCGCATTTTTTTGCCAAGGAAGAAGCTCCCTCAACAAGCATATCTGCACCTTTGATAAGAAAAACAAATCCAAGGATAAAAAGAAGTCCTATTCATCGTGACATCGACAGTCTTTTGAACAAAAAAATAAAAAATCAGAATTAATTTTCAGCAGATCTTTTGACTCTATTCATAATCGCGAATCCTAGCCCATTTTCTGTCAATCTCTGAATAAAGACCGTTTGTATTCCTGCTTTTTCGCAGTCGTGATAGAGCTGGAAAAGGGTATGTGCACAGCTTGCGAGGTTGGATTCACTTCCTCGTTGGAGTTTTGTGAGGAATGGAGTATGAGGATCGAGAGAGTGGAAGTTTTCACGCTCAAATCGCTCTGTCGTGGCAATGACTGCCGTCGGCACATCAAAAGATGTAGGAAGCTCGGTTTTGTGATCAATGATGGTGATTTTACCGGGGATACTATAGTGTCTAAATCTGGTTCCTGGGGAAAGGTTTTTGTCTTTGCTGGTATATTCCACGGAAACTGGTTGCCCCTCAAAAAAAGCTTCAAGATCCTCTTTGGTAATGAAACCCGGTCTCAAGATTCAGATTTTTCCCTGATTATTTTCTTCGTCCACCTTGATAACTGTCGACTCTATCCCGTAATTTGAGTCTCCACCGTCAATAATGAGGGGAATTTTGCCTCCCACGTTGTCCATCACCATTGGGGCAGAAGTCGGGCTAGGTTTGCCGGAAATGTTTGCACTCGGAGCTGCGATTGGCAATGCTACCGCTTTGAGAAAGTTGTTGGCGATTTGGTTGGACGGCATTCTGATCCCTACGAAGGGATTTTGAGTCACCACTTCGGGGATTCCTGGTTTTTTTTTGAGCAGGAGGGTAACTGGTCCAGGGAAAAGCTTGTCAATAATTTGCTGCTGGAAGGAATTTTCCACGATTGCGAGGTCTGCGATCTGGCTTTTCTCGCCAATATGGACGATCAGAGGATTGCTGCTCGGTCTGCCTTTTACTGTAAAGATCTTCTGCACTGCGTCGCTATCAAGAGCGTTTGCACCGAGCCCATAAATCGTTTCTGTCGGGAAGGCAACCAGCTCTCAGGATTGAATCAGGTTTTTTGCCTGTTGGTAGAGCTCTGGACTTGGTTTGGAGATAAGCGTTTGCATAACAGAAGAGAATGATAAATCGGCAAGAGTGTATATGTTTGGTACAAGATAGCAAGAGAATTTTACTACAAAACAAAAAAGCAGCTCGTATGCTACCTTATTTTTTAGATGGGGCGATTGACGGGAATCGAACCCGCGACCCTCGGGACCACAATCCGATGCTCTAACCAGCTGAGCTACAATCGCCATCCTAGATTATCGGTCTCTGAAGAGAAGATTGAGCTAAGCTCGCAACAGGCAAGTCCTCAGACCAACCGCTCCTCTTGGACTTCAGCTCTCCGACGTCACTCACTATACTAAAAAGGGAGACGATTGCAAGCGATTCATTATTTTTGAGAGAGAATGAGAAAAAAATCTGACTTTTTTTGACTTTTGGAGGATTTGTCAGTATACTTGTGCAAGAGCATTTTATCTACACTGAGCGGACAATGAGTAAGCTAGAAGTCCAGAAACATCCCGAAAAACAAGAGCAGTCAAGAGAGCTTAAAGCACTCTGAAGAGATATTGCTTCGGAACATTTAAACAAAGCGGAAGAAAATTCTGATACCGTCGTCAATACAGAAACCTTAAAGCTAGGGATCATCAATGAATATTTTGGGACTAAAGATATTAATCAAGCTGGGAAACTCCTTCGTGATGCTGGTTTTGCGTTCACTCCTGAGCAACTGGCAGCTTTTTCTAATGCAAAGGGAAGGGCTGAGGAAGCCCTGATCAAGGGGAATTTTGAGCTTACTCCCCAGCAGACACAGATTTTAAGCCGCATAAAAGAGCAACTCCAGGCTTCACAGCAAGATAATGATAATGAAGAGGAAATAACAGCGAAAGAAGACTGATCTTTGACTCCGGTGCAGCCTGAAGGAAAGAAAGCTAAGGAAACTGAGGCGCAAACTTTTAGAGATCGTTCACAGTCCACGTCTCGCTCTTCGGATGCTGAACAAAAGTCGGCTCAGGGCGTTAGCACTGTGGAAACAACCAACCCAGATACTCAAGAAAGCATTAAAGTTGCCGCGGCTCAAGATACTGCAGAGTCGCTTAGCGAAGATGCCAGAGCAAAAAAGGCGCTTGACATCTTGCAGCAGCTTTCTAGGGAAGTAAAGCAAGATGAAGGCTGAAATGTCGTGCTTAATCCTGAGCAAATACATGCTTTTGAGACGATTGATGATTTACTCGTGGCTCCAGAAGCACCCGTAGGGACCGCATGAATTTCAGAAAGAACGCTCCTCGTGCATGATAAGGCAACTCGTAAAAGGGTGATAAAATCCTTGAAAAAAGAAATTAAAGCTGAAAAGAAAGCCAAATCTGATATCAGAAAGATTGCGAAGCTTGAAGAACAGCTTGCCTCAGTAAAAAATGACAACAACTATTATTATTCAAAGGCGAGCCTTCTAGAGCAGATCAATGCTGCTTTGGATCAGGGAATTTATATCAATACACCGATTGCTTTTCTCAAGCAGACTCCTCTTATCGTAGATATTTATAAAAATAGGAAGAAGATCAAGGGAAATGCCAAGACGGAGATTTCTTCTCAAGGGAAAATGGTCGATGTCTATAATCAAGCAGTAATCACAGGTCAAGTTCATGAGGTAGGAAACTATGTTCAGAGCTGTGGTGGCTACGCTAATGTTGCAGAACAGTGGCTTCATGAAAATACGAAAATGTCACATTGACAAGCTAGAAATGCTGTAAATACACTCACAACAATTGGGATGATTGGAGCAGGTGCGCTAGTATTGAGATGGCTCTTTACCGAAAAATGAGAGGATGGGAAAAGAAAATTCTGATTCTCTTTCTCCAAGATTCTCATGGCGGTAGGGATTCCATTACTTGCTAATTTTGGTTCTCAGGCAGCTACAGGAAGATGAGTTCTAGAAAATCTTGCTCTTGCATGGAAAACGGGGAAACTCCCACGAAATAATGAAGTAATGAGTCAGTCTTCAACTGTTGAAAAAATGGCAATTGGACAGAATATGTTGCAATTTGTGCTCTTGGGAGTGCCTTTTAGCACGCTGGCACAGTGCTGCTCTCCAGCAACGGGGATAGTATCACGTATTGATAGTAGTAAGCTCAAAACCTACTATCTCGGAGAAATAGAAAAAGCGAAAGCAAATCGTGACTCAAAATCTGAAACCATTCTGAGAAGCCAACTTGAAGCATTGGAAAGCATTATAAGTGATAAAGATACTGAAAAACGCTTTTCTCAGGAATTTGAGAAGATGCAGCTCCAGGCAGATGACCTTGCTAATCCAGCATATGCACAAAAAACCCTGAATGAAAGACTTTATCAAAACTCTCAAGCCTTTAAACTTTTGGAAAGCTACTGTGCACAGCACCAGCTTATGATCAATGAAGAAGCGAGAAAAGAGATCGAAAGTCAGATTTTTAATACTGGTAAAGCACCTACAGAAGCAGACTTTAAAGCGATGCAAGAGAAGGGACTACTTATCCCGAATAACGCTAACTTTGAAGAGCTCTCTAAACTCAAGCTCAGCGATGCAGACAAATCCTGACTTTATTCAGACTTTTTGAAGCTAGCGAATAAACCAGAACATCAAGGAATGCAGATCAAGGCACTCAATGAAGAGGAAGTAGAAGTCTCTTCACACGGGAAATCGATCGTCCTCAATATGCAGAAATTGACGCTAGATGGCTTTAAAAACAGTGCCAACATCCCTATCCCTCTCGGAAGTAGGGGGGAACTGCTAAGAGTCGGGCTTCTAGTCAATATGTTGAGAGCAAATGAAGAGATTCGAGGAGAAAAAGTCACTCCTCGCTATGAAGATAAACACGATATGCTCCGACCGTTTGACCTTGCTCATGGACGAGAAGTAGCTTGGAAGGGATTAGGAAGTATCGTCTTCAATGGATTTGAGAAGAGACCGGTCGTGCTAAGTAATTTGATTCCATTTTTTGGTGAGATGGCGAGCGAATTTCCGACGCTAGGAAAAGCGCAGAATAGCGCATTCTTCGTCCAGTATTTGAACCAGCTCCGGAAAAACGATCGTTCTCAATAGTTTAAAATCAGATTTTTTACCCTTTTATATAGAATATTATGACTCCAGGATTAACCTCTTCTGAAAGTCCTTTGAATAGGCAAGAAGCTCCTGCTAAAGCTAAAAATTCTCTGAAGAACCTTAAAAATACTGCAGCAATTATTGCAAGCTCTATTGCTATAGTATTAGCTACTACCAGCTGTAAAGGTGCAGCAGAAAAATATGAATCCTGAGAAGCTGAGCAAGAAATCGCAGCTAATCAAGCGGCAATTGACCTTCTCATTGAGCAGTATAATGATTATTTCACAGACTACGAGAAATGTTTTGATGATATGGTTGCCTTGCAAGCAGCTGGAGATATTGGAGGCTACAAAAAAGTCTTTAAAAAAAGTAAACAACTCGAGAAAAGAATTAAAGAAATTGAAAAACAAATTGATAAAAAATCCATGAAGACCCTTGACCTTTCAGATAAGTTTTATAAAGGGAAACAAAAAGCTGAAGAGGAAAAAGCTAGGAATGCTTCTTCCTTCGGAGACTACCAACCAGCAAAACTGCAAAGAAGAACCTTTTTGGAGGACTGAGAATAGGCAATAGGCTTGTATTTTATTAGGAGAAGCAGGCAGAAAGTTATTGATTGGTCTGCTTTTTTATTCGCATTCCGTCTCAGAAACTATAGAATTTGTATCCTGCTTGGATTGCTCTCTGGTAGGCTTCCAAGAGATTTTTTCTTCCTATAAATGCAGAGATCAAGATCATTAATGAAGATTTTGGGAGGTGGAAGTTCGTAATCAGTTCATCGGTTAAAAGAAAAGGGATTCCTGGTCTGATAAAGAGCCTTGTTTGGATAATCATCTTTTCTGGTATAGAAGAAAACTGAGTTTTCTCAACGCACACCTCCTGCTCTGGAATAAACTCTTCAACCAGTTTTTCATCTATGGTTTTTGTGAGGTGATCTCGTCGATCTCTGGTAGCATGATCAACGGCAATGTTCAGATTTTTCTTTTTAAGAAAACGTCGGATATAGGGTAAAGACTCCAAATATCTGATCATTGTCGTCCCTATTGGGAGGAAACGTTTACCTGCCAGCTTTGCGTCGGCGATCGTCTTTCGGATATCTGACTCTAGGATCATCGGCTCAAAGTGCAGTTTTTGCTGCTGAATATGCTCTTCATAAACAGGTTTGAAGGTTCCTAGTCCTACATGCAGACAGGGAAATTGGAATTCTACCCCTTTCTTTTCCAGGCGTTTTACTAGTTCGGGAGTAAAGTGTAAAGATGCCGTTGGAGCCGCTGCTGAACCGAGTTTCTCCGCAAAAAATGTCTGGTATCGCTTTTCTTTTTCTTTTTCATAGCGGATGTAAGGAGGAAGTGGCATTTCTCCCTTTTTTTCCAAAAAGTCAAAAATCTGAACACTCGTTTCGGATGGATTTTCGCTCGAGATTTCAAAAAGGATTCCATTTGCTGTAAATTCGAGACTTCTGAATACGACTCCCTCTCCGAGGAAGATTTGGCTTCCTGGTCTAAAATTTTTATTATCGGAGACTAAGCATTCAAACTGAGTCTTTGAATGAATAGCATAGATCAATAGTTCTCCTGTTTCTAGGAGAAGTTCTTGACCAGTTTTTCTGCGGATTTTGGTATTGTGAAGCGGGATTCTCGCCTTAAAAACTTTCGTACTATTGAGGAATAGCATATAATGTTGATCCAAATAATTTGGCAAATCGGCAAAAATCTGATCTTCATACAAAAGATCTCCTGTGGCTTGGAGAGAACATATCATGAGTTTTGCGTTATGTTCAGGCAGTTTTGGCTGCTGTGCAATACACGATTCTGGAAGGATATAGGTATAACTAGAGAGTAGTAGATCTTCCTCCATGATTCAGATTTCTTCTAATTAAAAACTCTCTAATTGCAAATAAACAAACTGCGGACATTGCTCCCAATGTGTCCATGAGCATATCTTTTTGTGCATCCCAAATATCCCCTTGAGACCCTAGCACAGCAATCCCAGCTGCCGGATCAGCAGAAATAGCATACCACCACTCAAAAATTTCGTAAGCTCCAGCAATTGCGAGAATACTTGTAACTCCAAAAGTTGAAACTAGCCACTTTTTCTGGATTGATTTTGATCTTCGAAGGTATTCCACTATTGGAAAGGCATAAAGTCCTATGATAAAATGCGCAATCCTATCATACATATTCCTCTCCCAGCCAAAGAGATTATTAATAAATCCGAAAGGAACCAACTCGAAACTATAGTGTCCACCTATTGTATGAAGGAAGATTCGTAAACTCATAAAGAAATAGGAGAGATTAGAAAACTTCCACCAGCGAAAGGATATTACCAAGAAAGCTACTACCGCCATCACAGGAATATTTTCTGCCCACCAAACAGATCTATCATGCGGATTAAAAGCACAAACAACGAAGAGTACCAGATAGATTACTAATAAGCAGCAAGGGAATCGTGGTATTTTTTTGGACATGGTAATTGCTATTTTTTGGATAAAAAAGCTTTATTATTTTCTCTCCACCAGCTATGAGTTGCTAACTAAAGGAGGCATTGCAAAACCTCTTTATGTTCAGCTCGTTGTGTACTATAGGGAAATCATCTCACTAAACAAGAGATGATTTTATTTTTTGATGGATTTCTTTATCTTTCGTTTGTCAGAAAGAGGCTTGTTTATTGACTTTTTTGTTTTGAGCTCTTGGTATTGAGGCTCTCTTTCCATAAGGAGTTTATGAATGCCCATCCTTGTTTGTGAGGCATTACAAAATTCATCGATACTCAGGACTAAAATACCTTTTATCCCTTGTATTAAGGTCAGTAATTCATATCCGTGATCTCTAATTTTTTTGGAGTAGTACTTTTTAGCAGTTTGTGTCAACTGCTTCTTTGGCTTTATAAAGTAGTCGATTTCTAGCGAGGTCATTGCATTTTTTCTCTGGATGGGAGAAGGCTGAAGATTGTCTATCTTCTCGGTATTTTGAGTATTCAGAAAAAGGAGATACTCGCAAAAAATATTCAGATTTTTTTCTAAATTTTTAAGTTTATATCGATGAAGATATCTTAATGAGGCAATTACCTCTTCCTTGAGATATCCTGCGATATGAAGTGCATTAATCTGCACGCATAAAGATTTTAGGAGTTGCTCTGTCTTCGTCATGAAAGCATGAAAAATAGGATAACTAAAGGAATTTTCTTAACCAAGTGTAATAATTTTTACCTTGGTACGTTCTTTAAGATATTCCAATAGAGAAAGATCATGCGTAATAAGGACAATAGTATTTCCGATCTGATTTGCTTTTATGAGAATATCAGCGATTTGAAGCGAATTTTCATGATCCAAGTTTCCTGTCGGTTCATCTGCGATAATAAAGTCTGGGGCATGAATGATTGCTCTTGCCATCGCGATTTTTTGCTTTTCTCCTCCACTGAGTTTTTTAATTTCCTTGGTTTCAATAGAGGAAATATCAAGTAAAGTATTTACTGCATCATACTTTTTTTTGATTTCTGCGAGAGAAAGCCCTTCTAGCACTAGCGGATAAATCACGTTTTCTTTGGGGCTTAAACTACTGATGAGTTGATAATCCTGAAAAATGATTCAGATTTTTCTCCTATATCTTTGGATTTCTGCATCAGAAAACTCCGCCATATCCTCCATTTTGTAGTAAACGGTCTTTTTGTGGGGTTTGATTTGTCAAATCAAAAATTTCATTAGCGTTGATTTTCCCGTTCCTGATTTCCCCGTAATAACGGTAAAATCCCCCTTCTCTAATTCTCGGTTAAATTTTTTGAAAAGCACTCTTTTACTGTCTAAGTATCCTCGAGTCATTTCCTTGATACTAATAAGAGGAATATGGTTTTTTTCTGCTTTCATTTGGATATATGTCATAAGAATAAAAGTATGAAATGCTGATACCTTTCAGTCTTTCAGATTATCTTAACTATACTCTAAATTGTCTTTTTTTTCAAAAAAATTCAACACTCAAGAAGGGATTAAACTTGACATATTTGCATTTTAATGCTGGCTAGGAAAACTAGCAAAATAACATCTCATTTCTGTACTTTTTGAGATAGTTCTCATGTATTATCCTCTATCATTGTAACATTCTTTCTTATGCGATTAAGGAAACCAGGCATATTTTTTATTTTGAAAAATGGAGGCATAACGCCCTCCATCTTCCAGTCTTTTTATTCTTTTCATCTTTATAGCTGTATCTTAGGCTGCTAAGGCAGCGGTTTCTTTTTTTGGTGAAGCAGGCTTTTTTTCAGCTTTCTGATTCCAAGTTGGGATTTCCTCTATAAAAGCTTCAAACTCTGATACTTTATCTGCGGGAATATACGCTTCTAGTGCCATAAGGAAATCGCTATTAATTTGCTTCAACTGCTCTTCTAATTCAGTCTTTTCTTTTTCGGAAGCTCTTGGCAATCTTTGGAGGATTTGCTCCATTTTTTGCTGTTTTTGGACCTGAAGGAGAGCAATCTCTTCTTTTTGTTTGTCTGTTAGGCTATTTTCATCTAACATTCCCCATAAGAAGCCTAATTGTTCACCTTTTAGCGTATTCTTTTTACCTTTTTTTTGCTTTTTCCCTTCCTCTTCAAGGAGCGAGAGGAAATTCTGTGATTGGAACTGCTGAAACTCTGCTTGTTTATTTGGAGCAACAAAAGGGGCAAGATTCTTGAAAAGTTCGATAATAATTTGATCCTTTTGCTGTTTGAGTTTTGTCTGCTCTTCAGTAGTATTTTCTTGGCTCTCTGAAGTGAAGAGAATTTTGAGCTTGTCTTTGTATACTCTGATAATTTCTGCCAACTGTAACTTTTGATTTTCAGTGAGCGTGGCATAGTTGATCCGTGCGAGACTCATTGCTACTAGTCCCCCCTTTCACTTGGACAATATTTTCTGAATCTGCAACTTCTGAGCCGCTGGGATTAAGTCTAGGATAGCGTCTCTGATCTCCATTTTTCTCGCCTCCCACTGCATTTTGAGGAGGATTTTTTCTCTATCGGTAGTTGCATTTTCTCGCTGTTCCTGGTAAATACTCCTCTCTGTTGTGAGCAGATGGAGAAGTGCGTCAAGCTGATTCTTTTTTTCTTCACTAAGTGAAAGCTGACTCCAGATTGTTGATTCAGATTTTTGACTTTGGGAAGCTTCTAGATTTGGACGAAGATAAGGAAAACTAGGCGCCTCAAAACTCTCTATTGTCGCTGCCCGGGAAGAGAAACCCGTCATCGTGATCACTGAAAACACAACCACTGAGGCAAGAAATTTGCTACTTTTCATCGGTAAGAGTTGAATGTAAATCCCCAAGGTTGGGGGCCTGCAAGCGAGGGGGAGTATACCCTCTTACCTTAAAATAATCTTAAATCTATATAAAGCTAGTTTTTGAGCCTATATCAAAAGCCCTTCACTGTCTCTATGGAATCCTTTCAGATTTTTTTTCTGATTGTAGAAAGATAGACATCTAATTTACCGTCTTCTTGGAAACCAGCCTCTCCTCATCGAATTTCTTCAATGATTTCTAGCCTAGAAACTGCGATCCCGTGATTTCTCATTAGAAGCTCTAAAATCTGCAATTCCTTGAGAGAAAACTTCAGTTCTGATCCTGCTTTAAAAACTTTTTTCTGAGAAAGATCCAGTGTAAGATCTCCAAATACTAACGAAGTCCTTTGAGGAGCTCTTTTCATCAGTGCGAAAATTCTCGCCTCTAATTCCTCAAGATCAAAGGGTTTTACCAGATAATCATCAGCTCACACCTCGAATCCCTCCAATTTATCTTCCAATTGCCCTTTTGCTGTCATCATCAAAATTGGCGTCTGAGGCTGGATTTTTCTAATTGTTTTTGCAATAGCGATTCCGTCTGTCCCGGGAAGCATAAGATCCAGTAAAATTAGATCAAAAGTCTGATGTTTTACTACTTCATTTGCATAGCTTCCATTTTGGACCCGCAAAACCGTGTGCCCATCAAGTTCAAAATATTTTTTGATATTTTCTCAGATCGTTACGTTATCTTCTACCAAAAAAAGAGAGAAACCCATCCTTAAAATTTTAAGATAAAAGTAGTACCCTGCCCCAATACACTTGAAACTGCAAGCTGAAAGCCCTGTTTCTGCGTAAATAATTGTGCTAGATAAAGTCCAAGTCCGAAGCTTTTTGTGTCGGTATGTGATTCTTCAGCCTGCCAAAACCTCTCCCATATTTTCTCTTGTTGCTCCTTAGAGATTCCTGGTCACGTATCAGAAATTTTCAATTCTTGGTCATTGAGGGAAATTTGAATCGTTCCCCCTTGAGGTGTAAATTTACAGGCATTAGAGAGAAGATTACTCACAATTGTGGTTCGTCCTTGGGGATGCACTTTTTTTGTGAGTGATGGAGGAAAATCAGAGATGAAGTTCAGATTTTTTTCCTGATAGGTAAGCTGATACTGCTCTAAGAGTCCTTTTAAGCTCTGAGAAAGGTTAAGCTCCTGCTTGGGAAGTTCTGTTTTGTCCTCGATTTGCGTGATAAGAAGGAGTGTTTCTAAAAGCTGATCCATAGATTTCGCTTCTGCCTTAATCTTTTTGATGATCGCTTCATAATCCTTAGTCTTCTTTGCCAAGTCTGAAAGCGTAAAAATCGTCATTAGCGGGGTTTTGAGCTCATGAGAGGCATTTTTGATAAAGTCCTTGAGCCCTAGAGTTTGCCTTTGGATTTTAGCTAGTGCAAGATTAAACTTTTGACTCACTTTATTGATTTCATCCTTGGGATTGCCTTCGACTAAGAGTGGTTGATTTAGATGATCAATATCTAGTTTTTCGAGAGCTTGATTCAGTTTTTTAAGCTTTTTAAGTGCTTTTTTCACAAAAAGAAAAGAAAAACCATATGCAAAAGCTGATCCTACTAGAACAAGAACCAAAGTTACCCGAAGTAGTGCCATCTGAGCTTCTATCTGTGAAGAAATATTACGGATAATGACTTTATCTCACGCTATGACATAATGAAGATAATAATCGTCGAGCTTAGCTACTCAATTCCAGAGTTTTGCATCCAGCAACTCTTCGACCTCTGGATCATCCGGATCGAAACGGAGCTCATGGCGAGGAAACTCTTTATGAAGAGGGATATGCACGGCAAAAGAGGACTCCTCATCGTCGTCATGACGAGGACGTTCCGGATCTCGTAATGCATACCAACTGAGAAAAAAAATGATATTTACCAAAATCCCAAAAATACAGAGCAGAATTGCCGAAAAGATTGTAAACTGCAGTGAAATTTTTTTGGAGGTTGTGAGGGAATTTCGCATGACCTGTAGGAGCAATAGCTAAAATCGAGGAACTATAACTATCTAGCTTAAAAAAAGCTTAAATTTTCCGCTCTCCATCCACAAAAAAAGCAATCTTTTCTCATGAAACAGTTTCTCCAGTGATCGTCAAGCTTTTGTCACCCACCATAAAATCTACATGTGTAATACTTTGGTTCATCCCTGCCTTTTCTTTGCCTGCATCATCAAGAGTTATGCCGTCCTTGATTGTTGTAGGATAGGAGTTTCCCAGAGCGAAATGGCAACTTGCGTTCTCATCAAAAAGGGTATTGAAAAAAATCAAACCTGACTGACATACCGGAGAACTCATAGGCACAATAGCAACTTCTCAGAGTCTCCTTGCTCAAGGATCGGTTTCTAAAAGGGATTTCAACGTTTCGTATCCCTTTTTGGCGTAGAAGTCGACAACTGCTCCATCCTTAAATGTAAAACCGAAATCTTCAATCAGTTTTCCGTTGTAATAGAGTGGCAAAGTCGCTCCTAAAGTTCCATTTACTCCATATTTATGAGGTGCAGTAAAGACCTCCTCTGTAGGCATATTAGGACAAAATGAAATTCAGGACTGCGTTTTCTCTCAAGCACAAAGTCGAATGTGTCCAGTAGGAAGCTCTACTGTTAATTCCGTCTTGTCGGCATGGTAATGCAATTTCTTGAAGGCATAGTGATTGAGTTTTTGAGCGTGTGACTGCAAGGTTTGAAGATGATGTTTCCGAGCCTCGATGGGATCTTCGTGATCGATTCTTACCAAGGCAAAAATCTGGCTTCGTAATCTTTCAATACTCTCTTCCGCAGAAAGATGAGGATATAAACTTTTTGCCCGTCATAGGTTTGGAACCGCAGCGATAAGTCGATTGATTGCATTTGAGGAAATGAGTTCTAGCTGCGCTTTACGAGCAGCTCTTCTCGCCTGAAGCTCCATATTGAGCAACTCTGGCTGCACTTGACTAAAGAGAAAAGGGTCTTCGGAAAATAAGGTCAGCACCGCACAATTTTTTTTAAGTTCTGTAGCGTAGCCATCTTCTAATCGCTGAAGCGGTTTTCCAAAGCTTGCGACTGAAGCCTTTTGATATTTCAAAAGATCGAGTTCATCACTCCTCCATTGATAAAAAATCTGATCTACTCCTGCGTTATATGCCTCTTCTACTACCAGTTTAACAAAGTCAAAAGCATCAATCGGAGCTTTGATCAAGAGATTTTGTCTCTGCTGTAAATTGAGTCCAAACTGGATTATCAATTCAGCATATTTTTTCTTTTTTGCTTGAAGATTTTGCATTCTGAAGACGGCAAAGAATTTAAAAAACTAATACTCAACACCGATTGCATAATTTCTCAATAATTTAGAAGCACCAAAGGATCGAGACTTGAGAAGTACTGCATAGGGAATTTTCACCGTTCCCTTCATAAGGTATTGCTCTGTCACTCCTTTGGTATTGGAATCATAGACGTAAAAAATTTGCTCTCTATCGTTGTATCCTCGCAAAGTAATATAATGCCAATGCGTAAACGCCTTCACCCAAGAAAAAGACAGTTTGCTTGTTAGTCCGTTTCCTATCAGTAAGATGAGTGGTCATTTTTTGAGTTCCTCTTTGAGCAGTGCGATTTTTGATGTTGCAGTCTGATTTCTTGCCTGTATTACTCTCGTTTTGAGTCCATATCTTCTTAGGATTTTTTTGAGGCCTCGTGGAGTCATTAAATAAGTCAATCTGGACCGTCGTCCAGAAGAATACTCGTGTATTGGTTTTGTAGTGCCTCCCTTCCCTTCGATTACCGCTTTTAGAGAGAGATGTGCACAGGTTCAAAACTTTGCCTTTCTATGCATGAATCCCTCAGGTGGCGTCTTTGTAATCAGGAAAAAATCGTCCTCTATAGGTTTGGTCAGATTGGTATGAGAAGGAGCATCAGCTAGAATCTTAAATAAGCGGTCTTGGGCAAGCATAGGTATGGTATTAAGAACTATAGTGTTAGGCACATTTGAGATTTAGTATATCTGGAGATTAGTTTTTTGGAGAGAAATATCAATCTCTTTTGTCTGATGTATGAGTTTATTTGACAAGAGAGAGGGAATATGTATGCTTTGGTCAATTTATTTTCTGTAAAAACAAGATGAAAAAGCTGATCTTTGGCTTCTGTATGCTTATTGGGACTTTGTGAGTTGCAACAGCTGCTGAATTTACCGATATTCAAGAGAGTGTTTACAAGCAGGACATTACTGAGTTGGCGAGCCTCAATATTGTAAAAGGCTATCCTGATGGGAGTTTTAAGCCAAAGCAGGCGATTACCCGTGCAGAGCTTCTAAAAATCATCTTACTTTCTGCGAATATTGATACAGCAGGAGAACATGAAACTTGCTTTCTTGATGTCGCTAAGGAAGATTGGTTTGTGGACATCGTATGCACTGCGAAAGCTATGGGAATCGTAAAGGGCTATGATGACGGCACGTTTAAGCCGAATCAGACAGTTACTTTTGTGGAAGGGCTGAAAATGGCGATCGAAGGTTTTAAAATCCAAACGAGAAACGTCGATTCAAACTTTTGGTATGCAAAGTATCTTGATTTTGTGCATCAGCATTCAATTTTTTCTCAATATGCTTACTATCCAGAACAGGCATTTAGTCGTGAGATGATGGCTCATTTAGCAACAATGCTCCTCAAATGACAATCAGGAAGTCGAGATTATAACCGTAACTTTCGTTCTCCTGGCTGCTGAAAATCACAACCTACTAACATACCTTCTTCCGTAATGCTTAATGGAACAGAAAGGCACTTTATCACCCATGTAGGACAGCATTATACTCCTTCTCAACCTGCAAAATTAGTGTTTGCCTTTCATGGACGCACCAGCTCTAATGATGGACTGGGGTATTATGGAATTGAGGAAGCTAGTGATGGGAATTTGATTACCGTTTATCCAGCAGGATTACCAGAGGATGGTCCTCAGAGGAATCGAAGAGATCCGTGAGATAAACTATGAAATTTGAGAGATTATCAGCTTTTTGATGCTATTTTGGATGAAATCTCTGAGCACTATTGTATTGATCCGAGTGAAGTATACGTGGTAGGACACTCATTGGGTGGGCGATTTACCAGCATGCTCGGATGTGCGAGAGCAACGAATATCCATGGTGTTGGTATCGTAGCGGGAAGCCCCATGCCGTTTCCTGAGTGTTCTGCACCGACCTCTGCGATCATCTTTCATAATCCGAATGATAACCTTGCATCTTTTGCATGAGGGGAGCAAATCCGTGATAAATATCTCAAACAAAACCAATGCTGACCAGAAACCGAAGTTTACCAGAACTCCTATGGGATGGACTGCGTAAGATATTCAAACTGTCTCCCTCAAGCTCCTGTAGTATTTTGTAAGTATTCTGAGGGTGGGCATATGCGACCAAACGGAGCTGCCAACATGATGCGAAAATTTTGGAAAGAAAAGTAGCATCTTCCTCCCCATGCATAGAATAAGAAGCTAAAACTAAAAAAAAGATAAAAAACCTGATTTCTCATCACCGTAAGAATTTTTGTATTGCGTTTTTCAAAAAACTTACCTCTATTTTTGGATTGGAAGAGTAAAAAATCGCTTAGTTTAGCCAAATCCGAGGAATCGGTGTCTATCTTACAAACGTTTTGAATGAGTTTCCTATGATGAATTTATATTTGCAGCCCTGTTTGGGCGGATTTCCGATCAACAATCGTCTCTTGCAGAGCTATTTGAGTAAATTCCCAAAGTGAAAATGAAACAAAACGACGATTTTGGTGAAAAAACTTTTGGCAAATTACCTGCAGAGATGTTTTGAGCGAAAAAACTTTCGTCGCTTCCCCAAAAATGATGTTTTTTGGGAAAAAATGTTTGGCAAATGGTTTCGTAGAGATATTTTGGGAGAAGGATCTACAAAGTATTGGTGTTTACAGACGAGTTTCTTGGGATATATAATGAAGAATCAGATTTTTTCTTTTTGCAATAGACAAAATAATGCTACGCACTTTCCTTTATTATTCTTTTCGAAGGAAACTTCTTTTAATTGAATTGAATAAAAGGAAGAAATCACTAGTAATTGAGGATATTTTCTTTTATACTTTTGCTTCCCTATCATGCCAGAAACCCTCAAACAACTTATCCTACAATTTATGGGTACCATATATCCTTGAATAGAGAAAGAAGCTATTTTTTTGACAGGTTCTCGTGCGATAGGTACCTGGACTCCAACTTCGGATATAGATATTCTTGTTTTTATTGATCCGAAAGACTACTCGTATTATTTAAAGCAATCTTTTGAACATGGTTTTAGAAAAGAAGGTGAAGAGGCGGGGCTAGAAGAACGATTTTCTTATGAAGGAAGCGAGGTTCTTCTCGAAATAAAGTTTATGACAGAACTCTATCTTAGCAACCTCATTGACTATTTTTCTTATAGCAAGATGCAATTGGTTAATGACAATCACGCCAAACACAGCTCGCTTAAAGCAGACTTGCAAAAATGGTTTGATCAAGGGTATGAAGACAGACTCATGCAGCATTATATCCAAGCATTTCAAGACATGAAAGAGCTTAAAGATATGTTCAAAAAAGAACAAAAAAACTTGATGCTGGATACTATCTTTATCAAGAAAGGAATATTTCTGGAAGCTGTAATGAAGCTTCTCTATCTTATAAAAAGACAAGGCTATCCAACAGTTAAATGGATGTTACCTCTCTTAGACTGAGAAGAGGGAACTTGGATACAAGAAATTGTTTCTGAGGTTAAATCTTTAGCTACCCTTGAAGAATTTCTTATCATGGAGGAAAAAATTAAGAGGCAAATCAACAAAAAACTCATGCCACAAAGACCTTATGTTGGGCAACGGTGGAAGTTTCTTTCTGCGTTTAAGCACCTTTAAGATCAAAACTTCTCGTTTTACGAAAGAGAGCAATTTTACCTCTGATTTTTTCGCTATTCGGTAGGTGCATAGCTGATTTCGATCTTTTCTGCTGAAGAGGATCACTCATTAAGCATCTTTTTGTATTCTTCAGCTACAAAAGCAGTTTTTTCATGGATCTTACGTAACATATCTTCATATCCTGTTCTCGTATTCATAGGAACCTGCTCTGCAATATCATCAGCAATCTTCTGGAGGAGAACATCAGGATTCTCTGCCTTATAATTATCTTCTGATAAATAGGATTCTCCTGCCTGATCGATCTCGGCTTCAAAGCTATTTTCTCAGGTTTTTTTGATCTTGACTTCTACAGCAAAGTCCTGATGACCGTCTTTGTATCCCTTGTAAATTACCGAAAAAGTAACCGATTCTCCCTTTGGATCTAGGGTAACTATTTTAGAATCATAGACAGAACAAGAGGAAAGATTCGCTGCAAGCACCGCTGATACGGCAAGAGTTTTTGCAGCATTTTTGACGGAAGAGGATATGCCTCAGAGTTTATTGAAAGAATTTTTGGATGGCATAATGGATTGAGGTAATAAATAAAGTATTAGTTGGAGTCAGGTTTTTTCTTTTTTCTTCGTCGAGCTAGATTAGAGATTCGATTATTCTTGCTTGGCTCCTTAGTTTTTAGATGCTCGAAAGAAAAGTCTGAAACTGGCTTTTTCGAGTCAAAAACACGACCGATTGTATTCCCGATATTTTTGAAAAAATTCCCTGCGATGTCACGGACATTGAGAAAAGCGTTTTTTATCGGATTAAGTGTGGCTCTTACTGCAGCTTCTCAAAGCATTCCGATTCCGATCCCCACACCAGCAGGGACTTGGACGAGCTTATTATACCGCTTCCCAGTATTTCGTGAAGAGAGAAGCACCTCTTTTAACTGATCTCCAGTATTTCTAGTAATCTGACCAATATCTGTAAATATATTTCCTGCACCTCTCAATGTGTCTAGTATCGCAATACCTCACGCACCGAGTGTTCTTGGAGCTCCAGCAAGACCATTTCCAACACTCCTTCCAATACTCGTTATGGTTTTTCCCATCTTGGATATAAAAAAAGTAAAATTAGTCTTCTATGTCAAGATTTCTCAGATATTCGTCACGAAAATCTTCAAAAATCTGAGCCAAAAAATCATCATAGTTTGGAATCAGATTTTCTATTTTTTCTAATGCTTCTGCTTCTTTTCCTGCGTCAAAAAGCTTCATAATATCCTTCCTCTGAGCTGGGGAAAGTGTCTCAAAAACCTTCATCATAATTCTATCCATAAGGATAGGCTCTATGTCAGACTTAAGCTCTTCAAAATCATCTTTTTGCGGATCAAAACCAGCTTCTAAAAGCAGATCATTGAGAAAGTCCGCTTCATGGGTTTCTTCTAGGTGTTGATGTGGCATCTTTTTCTTTGTAATGGGTAAAATAGCTTTTAAATTCTGATTTTTATTCCTTTTCTTCCTCAGAAAACTCTGCATTCATATATTCAGCGAGGAAATTAGCAGTGACGTCTTCTAGAATAGCATTATAATTCGGGACGTGTGTAAAAAGGTAAGCATCTAGTTCTTCCTGCGATGCTAATCCAGCATTTTCTATCTCTTCATCTGAGACAATCTCCTCAAGCTTATGATAAAAATCATCTTCAATTTTAAATTCTATAAGGTCTTTTTCCCAATCAGAAAGCTTATCTTCTACGATTTCTTCGATTTTCTCTACTGCAAACTCTTTTGCTTCTGCTAGATTTTTCAACATGAGCTATGATTATCAAGATTTAAAAGTTTGTTTTTATCCATAGATACTTTTGCGTTGGCTCTCAAAATAATGATCTTTAGCCTCTTCTATGAGATGAGAGAGACGTTCTTTTACTAAGGTTCCTACAACACCATCTTTTGGATCAGGCTTTATTTTTTCTTCGATAACTGCAAGATTCCCATCAATATCTTTTGAATACTTTCTTCGCCTTGCCCCGGCTCTTATATTCCTTCTCCATCCTTGAAGCATGCCTGTGTAGTTTTTGCCTGTAAGTGACATTAGGTGAAAAAGAACACGCTCTCCTCGATCTTCTGGGTATTTTTCTTTGATTTTCTGCACTTGATGGTAGATTCTTTTTAATCTTCCCCGTTTCCCAAAAGGCAGAGATTCTTTGAGTTTTACATTTTTAGTTATTGCGCCTTCAAGCAGATTCTTCAACCTGTCTGCCTCGCCTTTTTGAGATTTAGCAAAATCGACGATATTATCTGCCTTTGAAGTCCAGTTTTTCATCTCATTTTCAAAGAAATCCTTTATGGCACTATTTTTAGCATCCTTTTCTGCAGGCGTTGTATCGGGCATATTTTGGATTGCTTCATACCTAGATTTCATTGTCTGGGTCGCCCCTTCTGCCGATTGAAAATCATTGATACTATTTGCCTTATTTTTGATCTTCTCGTCTAAAACTTGGTTTACAAGATTGAAATAGGCGTCATCGTTCCCATCAATTGCGAGTTTTAGCTGTCTTTTGAGCTCAGCTTCATCCATCTTTCTTAGCTCTGCGTCTGAATGCTTATCTTTAGCATCTAGGGTACTTGCAGAATAGCTACTGTAATTATTGTATGCCATCTCTTAATTTTGCTCTGGAAAATAAAATTATTCTCCTTTATTATACCGCAAAATTTCTGTTTGTCAAATTTTGACCGCTTTTCTAGAATATAGCTACAAGATAGTTTACTCTGAATACCCTTTGACATAAAAAGCCTGATACTTGGATATAATCCACTCTTGAAGGCGCTACTCCTGGCATTAGTCAGCTTTCTTTTTTCTTTTTTTTAGGATTTTCATGGCATTTCTATTGATTGTTTCTTGACGGGCTTTTTGCTCCTTACGAAGAGATTTGAGCGAAACTCCCGGTGCAGACCTCTCTTCTTGTCCCTCGCCTCTCTCACGGAATTTGATGACGATAGGGACTCCCACGAATCAAAAAGCTCTCCTCAGGACGTTTTCGATCCACTTCTTGAATGAAAAATTCGCCCTTGCTTTGTGATTCACAAAAACCATAAATGTCGGAGCATCAACCTCTATCTGCGTCGCATACATGATTTTACAGGTTCTATTTTTGGTGAAGCGAGCTGGCCTCTGGATTTGTTCTTGGGAAAGAAGGCGATTGAGCTCAGTAGTTCAGATTCTTTTCTGATTTTCTTGATGGACTCTTTGCACCATTCTAAGGAGATTCCCGATTCCGTCCCCATTAAGCGCCGAAAGTGGGATAATCGGAATATGTTTTGCAAAATCTAGGTAAGACTGAGCACCTTTTACCATTGCATCAATCGCCTCCTTTTTTACAAGATCAGCCTTATTGAGAGCAAAAATCATCGGAAGCCCCAACATTGTCATTTCCTGCAAAAGCGTCATATCTCTATGCGTGATTCCTTGCGTACAATCCACCATAAAAATCACAATCGGTCTCATAAACTCGAGCATTTTTGAGGTTTTGTCGTATGCGATTTTTTCGATACCGTGGGTTTTCCCTTTTTTTCTGATGCCGGCAGTATCGTAGACGGTATACCATTTTCACTGGGACTTGAATTGTCCAATGAGGTAATCCCTTGTCGTCCCTAATTTATCCTCAACCTTTGCGAGTTCATGTCCGACTAAAGTATTGAGTAGCGTAGATTTTCCAGAATTTGGCTTACCGACGATTGCGATTCCGATTCCACGCTGTTGAGGGAGATCTAGCTCCTGGACATCTGGATGTTTATCTTTCCAGTCTCTTACAAATTCCTTGATCGAATCCTCCAATTCCTCCAGATTTCTCTCCTTTTTTGCACTAATTCCGATGACTTTTTGTAGCCCTAGAATAAAGTAGTCACTAATTGCGAGATCTCATTCATTAGCTTTCCGCTTTACATCTAGCTTATTCACGACAAGAAAAGTCTGATTTCTCTTGTCATTCTCCGTAATGAGCGAAAGAATATGCTGGTCCTTTGCCGAAACTCCGACCGTATCATCAATTACAAAAAGCAATAAATCTGATTCTTTCACAATTTTTTCAATAAAAGGCAGCTCATCTGAGAAATCATGCAATCCTGGACTATCAAAAAATGTTAACTTTCCTAGATCGTCAACCTCAATCTGATGCGTGATGATATCGGTTGTCGTACCATGAATATCCGTCACTATTGCCCTAAACTGCCCAATGAGGTGGTTGAAAAGTGTAGATTTTCCTGCGTTTGTTGCTCCGATAAGCCCTATTTTCATTTGCAATGAAAGTGAAATAAACTTTGGTAAGTATAGGGAAAGGTTAGAAGATTTGCAAGGGAGAATTTGAATGAAAAATAGACTATCGTGCTTTATATTCTTTTTTGAAGTGGTGATGAAAACGAGGCTCTTTTCTGAAAATAGCGATTTCTGGGTAGAGGATTTTTAATCTTTACAAATCATCATTTTTTCTTGGTTTGGCAAGATCATCTCGTCGGAAATTCAGTGTTTTTTTGTGATTAAGATTTTTTTGCATATACTTAGGTGATTACTTTAGTTGATTATATTTTGTGATGGTTTGTGGATGAGAGCAATCATGAAAGAAGCTTTACTCACAGCAAAAAGTAGGGAGATTTTTGAAAATTTTTGGTGTTTTTTTGATCGTTATTTTTGTATTTTCATCGCTATACACCTCCGCAAATAGTATTGGCAATGGAGAACAAACAACAGAACTGCTTATCGGATGGGGGAATCTCCTTCAGGTCGTCGCTAATGTAGCATACGTTATTCTCCGACCATTGGTAGCACTTGCTGGATTGGCGATGGATAATCAGCTTATCTATGGTTCTTTTATGGGATTAGATGCTTCGCTTTGGCAGATTTGGCAGATTGTAAGGACTTTTTCAAACTATGCACTTGGATTGATATTTTTGATCTGAGTTTTAAACTATAATCTTTCTCCGTCTAAAAAGGTTCGATCGCTATGATTTTTTAATAAAATTGGCTCGCCTACCGAACTTATCACAAAAACTCTCATTGCAAGTGTGCTAATCCAAGCAACATGGTTTATTATGATGGTGCTGGTAGATCTTTCGACAATTCTGACGTATTCTGTAGGAGGGATCCCGACGAGCATTATTACACAACTTGATGAAAAAGTCCATGATGTGAGGTTGCTTTCCACTAATGTCCTACTTAACCTTGGAGATGAGGGGCTTCAGAAAGATAAAACCGTCGATCAAGCGATTGTTACCTATTGGACTACGAAACAAAACAACAAAGATACAAACTATATCGCTCCGTGTAAAATTGCAGAAGTTCAAAAACAAAAGTTTATCGTAGGAAGGGCGTTTGATTCACTTACAGGAAGCAATTGATCTGGATGATTTAAGAAACTGACAATGATTCCTGGTTATTGTTCACATATTTGAGCCCTTTACTCTTTTGTGGAATTTGAACGTACTGGCGGAGAAAAGCAAGCTAATTATCAGCAAAAATTAAAGTCGTTTGTTAGAGATCTCGAGAGAAGCTCGGAAGACCAGGTCAAAAAGCTAATTGCATGAGGAATGATTTACCCTATTAGCCCATGAGCTTTTTTGGTAAATAGTGGGGGGAAGGCTCCCTCTGCGACTCAAGTTGATGGATATCAAGAGGGAGATACGAAATATGGCTACAAAGGTAATGGTTTAGCTGACTGTGATGTTTTTTGATTTGTTCCTGCGAAAGCGGAGTGAGAATGAGATAAAGCTAAGTATAAATGCCTCTATGACAAAACAGACATTTCCCTTTCTACCATCATCAATAAGGGAAAATCGATGACTTGACCTTTTGTTTCTCTGTATTCAAATATGTTTAATTTTGCTGATCTTTCGGGATACAAGGATTTGTGAGTATGGCAACTCTTTATTCTCGCACTGATCAATACAGGATTTTCTGTGCTTTTAATCTTACCATTGATAGCTCTGGTAGTTGTGCTTTTTGCGAGAGTCGGGATTCTCTGGATAGCAATAGCTCTATCTCCGTTTATTGCATTATTGAAAATTTTTGGTGGGAAGGCTCTTCCTGAATGGCTTTCTATTGATTGACTCATGAAGCTCCTTATCGCTCCTGTCTTAATCTCTTTTGCAGTCAGCATTTCTTTAGTTTTTATGAGTGCGCTTAAAAGCAGCTTAGGTAATGAAGCTTCTGGTCCCTCCAATGGCGGTGTTAGTACTGCAAAAGTACTCAAAGATATCTCTGGATTTGAGGTCGATCCTAAAGATGAATCACTCTGAATTCTTGGATTTATCAAGATACACCTCAATAATGGGCTCCTCAACCTTTCTTGGGTGGTTATTATGCTCTTTGGGCTTGGAATTACGTGGTTCCTACTCTTTTGGGCTATCAAGCAAACTTCTGTTGGGAAAGCAATTTGATCCTCTCTGCAAGAGCTTTGACAAAAGACCTTTATGAGTACTCCTTTTATTCCAATTGGTAGCAAATGACTTTCGCTACAAACTCTGAAAAATGCCTCTGTTGGAGGAGACTCTATAGCTAATAAATTCGCTAATAAACTACAAGAAGAAAGTGATAAGAATCTCAAGGACTGGCTTTCTCCCGACGATAATGATAAAGGCAATGATGATAAAGCATTTAAGGAAAAAAGTAAGGCCTTTTTTACTGAAGGAGCAGATCCGATTTCAACCTTTAACATTGATCACAATAATGTAAATTCCGCACTTGATACCTATAAAAACCTTGATGAAATCAGATCAAGCACTTATACCTCACAAGAAAATGCTACAAAAATGGCAAATTTGGCTGATACTCTGTGGAAAGGGCAGCTCGATAAATGAAATAGTGCGAAGGATGATCCTGAAGTAGCATGATCTGAAGCCTCAAAAATAAAGTGAGCTATGGAAGCAATTCTGAGTGACGGTACTATACAGAAATCTGGTAGAATCGGAGAATGGAGTAAAAATGCAAATATCAAGATTGGAAATAAGCAATACTCCCTTGATGCGACCAGCGGAAAATTAACAGTGAAAGGAAAGTAATTTTATCTCTTGTTTCAGTTGTTTATGCCTAGAAGAAAGCAAGTCTCTCAACGAAAAATCTGAATCATTCTTGTGATGATCAGTTTTTTCCTCGTTGGGAATGCTTTTGCGGCGGACATTTGTACTACTGAAACGACAAAAGGATTGGAAACCTTTAAAACTATTCTGACCGTCATAGGAAGTTTTGCTTCTTGGGTATGGGTTATTCTGGGTAGTATTGCAGGGAAATTGATGACCAATACGTTTGTTTATGGAGAGTTTATACATCTCGATGGACTTCTCTGGAAAATCCGACAAATCTCAAGAACTTTTGTAAACTATACAATCTGAATTTTATTTTTGATCGGTATTTTTAACTATATTTTTAATTTTAGCACTAAGGCAAAGAATCCTACCTCGTTCATTACTGAACTTTTGATAGCTAGTATATTGGTCCAAGCGAGCCGATTCCTTGTGATGGTGCTTCTTGACTTTTCTACAATAGGGATAGCAACGATTTCTTCTTTCCCATCACAAGTCATTAGTTCCAACTCAAATTATCGTGAATCTCTTCTTACCTCCATCAAAAGCGATCCTCTTCTTTCCAAACCGTTTAAGACGCCACCTAAGGGGGTTGAAATTAACGTATTTGCGGATGTATTTTCTCAAAAAGATAAAGAACTCCTCTGGAAAGAATATGATGTGGAAAAATTACCTGATGATTTTGAGAAAAACTTAATTGACTCGCTACTACCTAAATCAGACAATCTCGGAGGCCCCCTTGTCTATCTTGGATTCTCAGTCTTTCAAAGCCAAAATCAGATCATAAACTATGAAGCTAATCCTACTAATTGTATCGAGATGTTTTCAAAAGCAATTATCAGTCTTATTCTGAGCTCAGGAATGATTATCCTCTATGCATTTTCGCTTTTGGTTTTGGTTGCTGTGCTTATTATTAGACTGCTTTATCTGCGGATTTTTATTGCGGCAAGTCCTATTATTGTCCTACTGTCTAGAAAAAAATTAATAGAAGATTTTTTGGGATTAAAATTGAACTTATCTACACCTGCTCTTAATCTTGGAGATATGATTGTTCTTGTTTTTAAACCTGTATTTTTCGCATTATGGATCAGTATTATGATTCTTTTTGTTGTCATTCTTCAAGGGTTCTTTTTGTCACAAGATAACTGATCTTCTGAGTTTGACAATGGAAGTGTAAAAGTCTCTGAAACTATAACCAGTGATCAAGCGAGAAGTATTAATTCTTATCAATCGGAACTCACCATTTGAGAACATGCAAAAGTAGCGCTCTATCATGGGACAAAATCCTTCAAAGATATTATGCTTGCATTTATGACACTTGCTCTAATGTGGTATTTTGTTAAGCTCGCATTTTCATCTGGGAAAACAAAAATCTGATGAATTGATAAACTAACCAGTGGTGTTATGGAAACTGCAGAAAAAGTCGTTGGAAATGTAGGGATTATCCCAACACCAGCTGGTCAGATTGGATTAAAACAGGCAAAAGATATTCCTAGCACACTTATAGGAAATAAGCAGGGACAACTAGAGGCTGAAGAAAGAGAAAGATTAAACGCATTTTACAGAGACATTGGAATAGATCCTGGAGCTCTTTCAATGAGTGAATCTCAAAAAACCAAACTCAAGGAACAGGCTAATGCAACTAATCTTGAGAGTTATCCATCTCTAATCTTATCAATAAAGAATCAAAATAACGGGATTAAATTTAATGATATCCCGGTTTCTTCTTTGTATAATTGGATTCAGAATGTAAATAAGCATAGTTCTAAAGATCAGATTTTTAGTAAGTATTTTGGTCCAAACTGGCAAGATATCCCAAATATTAAATTAGAAACCTGAGACAAAGCTGAAGAGAAGTACTTAAAAGAACTCTTCAAAAGTGCTACTAGAGTTCAGACTTTTTATCAAAAAATGCTCTGAGGGAAAAACAACATTTTCGCTTATGAAGATCTTATCTCTGATCGTGGGATAGTCAAAAATTAATATTTCATACCACGCCTTTCACAATAGTGTTTTCAAAACTTTTAAATAGAAGAAAAATCTGATTCTTGGCTCGGGATCTTTGGAGAGACTCTAGGGTATCAACAATGGATTTAAAAAAACTTTTCCACGTTTCTAAAATATAATTAATACGCTCAAAATACGTCTTGCTAAAGCTACTATCAATGGGACTTCTGCAAATTTCTATTAGCTAAAGATACCTCTGTTCTCAAGCTATAATTTTACTAATCATTTCAGAGATAAGCGTGATCTTGAAAGAGAAAGAGAAAGAGAAAGAGAAAGAGAAATAAAAAAAATTGAAAAGATTGCAATTTGCTAGCCAGAAACTATAGTAGGAATATTTATATTTTCAAATGCCCAAGATGCTTCCCGCACTGCCTAATAACCGTCTTATTCTCAATGGACATAAAATTCTGAACCAAGATCAGCTTTTTGCCTCTATTAGTAAGAGGCTCAAGACCACTGTTCTTAACAGACAAGAGCTTCGCAAACTTCTCAGTAGCTATCAGTGATTACACATTGCCATTTGGCATGGGGACAGCTTTTTGGAAGAAGAAAATGAGGCAACCCAGAAAGAGATCGTTTTGCTCCTTTCAGAACTCGGAACCTTGAAAATTAGCGGGAGGAAAAAGCGGTAAGTTTTGACATTTCTCTTTTTTTGACTATACTAGAGGCGTAGTTTACCTCATTACTTTTTGATGCCAACTAATCATCTTTCCTCTCATTGAGAATGATTGAAGCTAAATCCCAAAGATTTAGAACGGTTGAAGTATGATCCTAAGGATTATGCTGATATTTTTCCTTATGCAGAAGCATTTAAAGCGATCCGCAGAAAAATTGGTCAGGATTTTTCTCTTCGTAAGATTGGTGAGGGGGCTTGGGCGAGTGTATTTGAAATCCAAAAAGACGGGAAATGAGTTTATGTGGTAAAGTTACCTCATCCTGAATATCTAGATTCTCAAGATATTAATATCAAAGATAAAGTGAAAGCTGAAATAAAGCTCCAAAAAAAGTTCATTCTTGAATATGATACCTGGGTCAAGAAACTGCATAAAAACTATCTCAGTATTGTTACTGATCCAACAGGCTTTAACCTTTATTCTACACTTTCATGACAAAAAAATGTAGAGGCAAAAAGCGATATTTCTAGCATTGAAACGATCAAAAAACAACAAGCTATTCTTAAGACCCTTCAGTTTCCCCGCCTTTTGGAAATATATCAATGAGAAATCCCAATTATTGTTATGGATAAAATCCCTGGTACAACTTTACGATTTGAGATCATGAGATCAGTTTTTTCTCCTTTTTTTGAAAGACATTTTGCTTTTTGAACTTCAGACAACCTTCTTATCAAATCAGACAAGACGCTGTATGAAAATTGTAAAGAACAGAATATCAGTGATAAAATGATCTCAAATTATCTTCATCTTATAGAAAGAGATTTGCTACCTAAAATATGAAAAAAAGCATTTCAGAAGGAATTTATCAACCCTGGAATGTTGGAATCTGATCCTACAATAATGATATCAAGTTTTATTAAAGAGTTTGATGGGGTCCGAGAGCCGGATCCTACAGTTTTTACACAGCTTTATGGGGATAGTAAGAAGTGAACAACACTTGAACTAGCCTATAATTTTTTCCTCAAGCATTTTCCTAAGCATCCAGATCCCAATTTGCACAACCTTATCATCACTCCAGACAATAAACTTTGAATTATAGACTTTTGATAATTTTATTCTTTACAAAGCAAGATTATGACTCTAAATAACCCCAAGGCCACTAATCAGCCTCCTGTTCAGCCGTGAAGCTGAAGTAATACTTCCCCAACCAAAGAAAATCTGAAAGAAATCCTCATTCCGAATCTTGATACCATGCGCTATATGGAAATGATGACGCTTTTTAATAGCAAAACTTATGATGAATGAGTTGTAAGAATGAAGAATCACACAAGAACCTTTTTGTATTTTCACAATCTTGCTCAGCTGTCTGATGAAGTGATCTTTCCTTACCTTGAGCAACTCCAAAAAGATTATCCGAATCTCCAAGGCTCTGTTTATCTCAGGCATCATAAAGGAAAAAAATTTATCTCACTAGAACCCTTATTCCCTGATTCTGGAGAAAAGAAAATTGCTCAAAAACTAGCTGATGAGCTGAAATCTCTTTTGAAACAAAAAGATAAAAATCAGACTTCCACTGTTTAATTTTCCTCCAAAAATCTCTTTTTCCGACCTTCTAAAATACGGTTCAAATACACGATATTATGCAGTCCGACAAGGCTTCCTCAGAGTGTTTCCTGTTCTCTGATGAGGTGGCAGAGGTAAGCTTTGCTAAAATTTCTGCAAGTGTAGCAATCGCAGTTCTCAGTCAAAGGAGAGAAATCAGTTCTAAAGTCAGAATTGGTTATTTTGATATTCCCTTGGTCAGAAAAGGCGATTCCGTGCCTTCCAAGTCTCGTTGCTGCGACGCAGTCAAACATATCTACTCCCTGCTCTATCGCAAAACGCAGGTCTTCTGGAGTTCCTACGCCCATCAAATATCTCGGCTTCTCATTTGGCAAAAGTGGTGCAGTATGTGCAATCACTTGCTCAATCAGCTCTCTACTTTCTCCAACACTCACCCCACCGATCGCAATTCCATCCCGAGCGAACTGAGAAAGGTATTCTGCGGATTGGGTTCTGAGGTCGAGATACGTCCCCCCTTGCACGATAGGGAAGAGGACACCTCTCGCCTCTTCGTATTTGGGCTTGAAATGTTCAAAAGCTCTTTTTGCTCGGCGATGGGTCATAGCAATATGCTTTTCTATGGTTTTCTTGTCAGAACCAGTAGGAGAGCAAACATCTAGCACCATCATAATATCAGATCAAAGCTCACATTGGGTATCTACCACATTTTCTGGGGTGAAGATATGCTTTGATCCATCATAAGGCGAGCGGAATTTTACACCCTCTTCAGTCAGCTTTATTCCTACTTTGTGAGTATGTTTTTGGTCGTTGAATTTTTGATTTGCTAGACCAAGAGAAAAAACCTGAAACCCTCCGCTATCAGTCAGAATCAGTCCGTCTTTCCAGTTTTCAAACTGATGCAAACCTCCAGCCGCCTGGACAATTTTACTTCCCGGACGAAGATAGAGATGAAAAGTGTTTGCGAGAATGAGCTTGATCGGCTCAATTTGATTTCCAATATAGTGTGAATCCTGCAAAAGATCTAAAATCAGACCTTTGATCGTGGCTTTGGTCCCCACCGGCATAAAAATCGGTGTTTTGAGGCTGATTCCATTGAGGGTGATTTCACCGACTCTGGCTTTCCCTTTTTGCTTGAGGATTTTGAAGTTGAGTTTTGACATGGTTGATGGCAAAGGAAATAAGAAAGATTTTTAAATTTAGACAGCGACATCCATAGGCAAAGAAGCATCTGGATCATAGCCGTTTATTACGGTGTCGGTGTATTCCCGTTCAAAAATTGAAGAGCATTGCTTGGTTTCTATATTTGGAAGGGGCTTTGGTATTCTAGTCAGCTGTTCCTGAAGAACAGAAATATGGTTCTCATAAATATGGGTATCCGCGAGAAATCCAACCAGCTTCCCTTCTTGTAGTCCTGCTTCCTTGGCAAGAAGATGGAGTAAAAGCCCATAGCTTGCGATATTGAATGGGAGTCCTAATCCGATGTCTACTGATCTTTGATTTCGCAGGAGGTTGAGTTTTCCATTGATGACGGTCACCTGAAATCCATAATGACAAGGCGGTAAAGCCATACATCCTAGATCCATTGGATTCCAGGCTGAGACGATCATTCTCCTGTCGGAAGGGTTCCTTTTGAGGGTTTGGATCAGATTTTTTAACTGATCTACACCCTGCTGATCGTAATTAGTACTAAAGGTCTGATACTTAGCACCGAAATGCCTCCGCTGAAAGCCATAAATCGGTCCAAGTTCCCTCTCCGCTTTCATCGCTGCCTGCGTCGCAGTATCATGACTATAAGGCACACGGTCAGGCGAGCACCATTCGTCTCGAATATGGTTATTTCTCGCTAGAAGCCGCTCCTTGTCAGTAATTCCCTTGATGAAAAATTCTAATTCGGAAGCCACAAGTCTAAAAGGGATTTTTTTGGTGGTCAGAAGAGGAAAGCCCTCCTGCATATTGTGCTCAAAAAAGGTTCCTGCGATGGCTTTTGTGCGGATTCCGGTCCTGTTGTCTTTTCGTTCTCCGTGGTCTAGAACGTTTTTGACAATGGCGAGATAACTTTGCATGGGTTGAAAGTACAGGAAATAAAAGTTATTTTCTTTGATAGATTAGGAAGTCTACCTCTTGTCCTGTTGTCTCTGAAATACAGACAAAATCTGACTCAAAACTGGGGAAAAAGGTATCTCCTTGGTAGTTTTTTCTGATTTTGGTCAGATAAATAAGATCTGCATCCGGAAGAAAGCTCTGATAGGTATAAGCTCCTCCAATAATGAAAACCTGTTCTTCTGGATCTGTGGGCTTAAAATCTGCCAAAAAGCTTGCCCTGTCTGGATAACATTCAAGGGAGGGAATCGGCTGGGAAGTAATAACAACATTTCTCCTCTGCGGGAGCGGTTTCCCCAGTGAAATAAAGGTATTCTTTCCCATAACGACAGTATGATTCAGGGTTGTCTTTTTGAAATATTGAAGATCTTCAGAATAATGCCGAGGGAGCTGGTTTTGGCATCCAATTACCCTATTGGGAGCCATGGCTGCGATAAGTGCAATCTTCATCAAGATCCTAACAAGAATAAAATTCTGATCTCAGTGTATGATTTTACTCCCACTTTTCAATCTAAAGAAATTTTTTGTTGGATTTGAAAATCGTATCGTGCTTCTTTCTGGTAGAATCCCCTGTTTTCTTCAGCTTTATTGGATTTTTTCTTTTTTGTGTCTGCTTTGTCTACCTTTCCCTTCTGTCCACACTGTTTGATGGAGACTTCTCGCTTTCCTGGATCGTTCTTGCACAAGAGAAAAATCATAGTTATCATTTTATAAAATCATAACTTCAATTTTATAAAATCATAGTTATCATTTTGAGAAATTATAGTTATAATTTTTTATCAGCGAACGGTAAGCTTTTTCTTTTTCGGGATTATTTTTGTGGAGCAAAAAGGAACACTCGTCTTTCCGTTCAAGGTAAGCCTACCGCTCTCTATGTGTTCTGATTTTTTTCTCTTTATTATCTCTAGAGAAAATGTTGTTTACTGCGATTATAAGAATTGGTGTATGGTTTATTTTAGCTTGTAGGGCTAGGAATTTTCTCATCACTTGCATTCTTTAAGCAAATCATTAAAATGTGTGTCGATTTACCTTTGTTTTATTAGATAGCTATGAGAACTTTATTAATCATTGCCATGCTCATTACTGGAATTGTCTTTATAGCAAGTGTTTTGCTTCAGTCTCCAAAAGGAGGTGGTATTGGAATGGGGCTCGGTGGAGCTTCTGCCGGAGGAAATGAATACTGAAGTAAAAAAACCATGGAAGGAAAGCTTAAGAAAATTGCACTTATTTCTGGTGTGCTTTTTGTGATTATTGTGTTGTTTTTACCCTACGTTAGATAATGCGATACCGTAGTTATGAGTCGAGTTCTCCTAAGAGAATGTTGGCTTATTTTTTGGGAGTTATGGCTGGGGTTCGAGTAGCATTTAGCCTGATCCTAGGATATCAATTTCTTGTCGAAAATGGAGAAAAGACCAATCAAAAGGGCGGAACCTTTGTCGAGGCATTCTTTGATCAGATTTCTTATCTTCCCTATCTAAGGAATGATGGGCAGAGTCTTTTTTATCAATCTTTGCTTTTTGATGCGTGTATTGATTATACAACTCTGAATGAACAAGGATTACAGAACGGTCAATGTAGGATTCTGACTCAAGATTATCAGACTTATTATGTTTCTGAAGTAGGAACTGGGAAGTCGTGGTCTGATGGCGAGCATCGAGGCTTAGACGATATTTTCTTTACCTATGACCGCATTATTCGTCAGAATGTCTGGAATATCAAGTCGCTTAATGCCTATCATGAGATTAGTGTTGAAAAAGAAGATGGGAAGATTAAGGTAAAATTCCCTACTAGCACGACGGACAATAATTACTTTTTTACGTTCTCTATCCTTCCAAGGCATATTTTGGAGCATGCTCAGATGAATGACTATACTTCAGCCTTTGCTGCTGGTCCAATTACTAGTGCCTGCGGGAAAATTGTCCCTAAGAGTAGTGATGGATTGAGCTTGGTTTTCAATCTCATGCAATGTCAGGATACCAACTTTGCTTACTATCAAATTAAAAAGTATCCAAGCTTTGAGGATCTTTCTAGACTTCTTCTGGAAAACGGGAAAACTATCGTTGATGCCTATACTCACCAAGTCCAGCTTCCTAACTATAAAACTCTTAAGGTTATAAAAACAGACCTTTTGACACTCTTCTTCAATACTAAGTCTCCGAAGATGAAGGTAAGGCTAAGAAGAGCATTAGCTGGATTGATCAATAGTAAGTTTTATGTAGGAGATGAGTATGGAAAATTCCTTTGGATGTATAAGTGAGGTCTGCTAAAAAAGTTTTACTCGGATGGAAGCAATATCAAGGAGTTTATCAACCGTGCTGCTCTTAGCGAAAAAGAAGGAGTGCAGCAGCAGGATCTCCAAGATAGTGGAGTTCTGGAGCTAAAAAAGTCAATCAGTATCAATGGGGTTGAAAGAAAGTTTGTTTTCTATACACCAAAGTCTGATGCTACTTTTAACCTGGAAATCAAGTTCTCCAACCAATTTGAAACCATAAAGATCAAAGATGCAAAATGACATGAATTCTCTCCCAAGAACTATAAGAAGACTGATAAAAAGGTGGTTTATCCTCTAGAAAATGGAAAGAATCTGAATCCAGGACTCAATCAGTATACTATTGTTGGTAGCATAAAAGGGAAGACCTATACGATTGCCAATATTGATCTCTATGTTCTTGCTACTTCGACACAAGAGAAGGACGACACGAGCGATTGGAAGGTAAAGGTTCTTTACTATAATAGTTTGGAGTCTAACTTTGCGGTAAAACAGTTGAGAACGATCTTTGAAAAGGCTGGTATTTTGGAGAACTTCCTCTTTGAACAGGTGAGCTCTCCTGAACAGTTTGAGGCAAAAATCGTCCTTGGAGAATATGATATTCTCCTCAACACTATTAATATGGGAATGAAAAAAGACCTCCTTAAAGTCCTCATGACTGATGATGTTATGGCAAACCCTTCTAAATACACCAATCCAAATCTTAGTAACTACTTTAAGCAATATACAAAAAATCCAGAAGACAGCCAAATTGCAAGTGAAATCAATGCAATTTATGCTCAGGATATGCCACTTATCATCCTCTGACATCCGTACAACTTTGTGAACATTGAGAAAAGTCTGATTTCTTCTGATTTTTGATCTGGTATTACGCTTTATGAGTACAACTGGAGAAAATACCTTTATTCGCAAGTTGCCCTCATCAAAAATACCGAGATTAATTTTAAGAAGATCAAAGACATTAGAGGCTTCCTTCAATATCTACAGTCTAAGTCTGGGGTATCCTTTCCGCTTCCTGAGATGAAAACAAATGTAGAGACGTGAGAGAAGGAACATGAATCATCCACTCCATCTCAAGTTGTTGATGGCGATCCCTTTGCTGGGCTCTTAAAACCCGCAGAATAGTATAAATAATCTTTTACATCATAAATTAGGGGAGCTTTTCCCCTTTTTTATTTGGGCTGCAGGAGAATGATTACCTTGAAAAATTCAAGGAAAACGCTATCTTTTCATTGCTTTATTTTTCAGGTGTCAATGCTTACATTTTGAGAAGTTCGAAATTATCTTCAACTTGCGATCATCCTCTTGATTTCTATTGGTATTCATGAGTTTGCTCATGCTTATAGTTCTTATAAGCTCGGTGATCCTACTCCAAAAATCCAAGGAAGACTGACTCCAAACCCTTTGAAACATATTGATCCGATCGGATTTTTGATGATTTTTTTGATTCATTTTGGGCGAGGGAAACCGGTACAGATTGATCCTAGTTATTACAAGAAGCCCTATAGAGATGAGCTTATTACAGCTCTGGCAGGACCTGCGAGCAATATCGTGCTTGGAATCCTAGGAATCCTCATCCTCATGATTTACGGAAAACTCCTCGGGACTTCAGCGATTGCACTTTTCAATGCAAATGATCTCGTTATTCAGTTTTGGATGCATTTTTCTATGATCAATTTTGGGCTTGCTGCCTTCAATCTCATCCCACTTCCTCCACTTGATGGATACAGACTGGTAAAAATCATTTCGCACAAGGCTGGCGCTTGGATGGAAAGAAATATGAGATATATTGCGATTGGTTTCCTGGTTTTAGTGGTATGGGGACCGCTTTCAGATGCTCTTTGAACCTACATTTCAAAAGTGTCTTGGGGATTATTTAGGATTTTTGCCTTCCCATTGAGTCAGATTTTCTATTAAAGAAGAGAAAATAAAGAAAAAAATCAGACTTTTAATTTTTTCATGATAATTCAATGCTTGAGAAGTTACAAACCATCATCAATAAATATGAAACCCTCAGAGAACAGTCGCTCCAGCCTGAGATTTTTGAAGATATGGATAAAGCCAAGAAAATCAATAAAGAGCTTGCAGGCTTAGAAAAAACTTATGATCTTGCCTGTGCTTACAAAAAAGCACTTGAAACTGAGCAGGGAGCATTAGAAATTCTTAATAATGAAAATGATCCTGATTTACTTGATATGGCAAAAGAGGAACTTGAAGAAGCAAGAGCTGCTATTGAAAAACTTGATTATGACCTCAAAATCGCCCTCTTGCCGAAAGATCCGAACGATGAGAAGAATATTTATCTAGAGATCAGACCTGCTGCAGGTGGAGATGAAGCTGGACTTTTCGCAGCAGAACTCTTGAAAATGTATCTCACGTTCGCGCAGAAAAAAGGACGAAAACCTGAAATCGTGGAGGAACAGCTTTCTGATATCGGTGGAGTAAAATTTGTGATGGTAAAAGTGGCTGGAGATTCGGTCTATTCTCTGATGAAGTTTGAGAGTTGAGTCCATAGAGTGCAGAGAATCCCTGACACCGAATCCAATGGAAGAGTGCATACTTCTACGGTGACCGTTGCGATTATGCCGGAGGCGGAAGATGTGGACATTCAGATTGATCCGAAAGATATCGTAATGGATACTTATGCTGCTTCTTCTTCTGGTGGTCAAAATGCCAATAAAAACCAGACCGGAGTCAGACTTCACCATCTTCCAAGTGGACTCATTGTAAATATTGGAGATAGTAAATCTCAAATGCAGAATAAAGAAAAAGCCTTTGCCGTTTTGAAGTCTAGACTCTACCAGATTGAGCTTGAAAAGAAGCAAGCTGAAGAAAAAAGTTTAAGAGGAAACCAGATTGGAACGGGAGATCGTTCAGAAAAGATCAGAACCTATAATTTTCCTCAGGATAGGATTACTGATCATCGTATCCATGAGTCATGGTCTAACCTTCCAAGTATTATGATGGGAAACATTGATGATATTATCGGCAAACTCGTGCTAGAAAATCAAACCATGCTGCTTAATCAAGAGATGCAAGGGGGGAGTTAGGATTTTTTTGAGAAAGTTTGCGTTCCTTTTCTAAATAAAGGAAAAAATGGAGGGCATGGAAGAGATTGGTATAGAGAGGAATCAGCAAAGGGCTTAGATGCCTTCCCAAATTCAGAATCAGTTGCACCAAGACCTGGAGATATTCTGGTCTTCTCTGGCGGAGGAGATGGGCATGTTGCTATTGTTATGGAAGTGACGAGGACTTATGTAAAAGTAGCTCAGCAAAATTCAGGTGATGGTTCTGCTCAAAATCCTCAGTGGTATCCAATTGGAGGAGAATTAAAAAGATCCGGTAATACAGTTGTTTGCTCTGACTCTAAGTTCAAAGTTACTGGTTGGATGCGGTATCAGAAAGAGTAATTATTAAGTATTCAAAAGGTCTCTATGGAATCTCATAGAGGCCTTTTCTCTTAAATTGTCGGGAGAGTATCCTAATTAGAACTTTAAGATCGTAAAATCTTTAAATAATATTCTGTCGACATCTCAACAATCTTTTCCTTCATATCAGTCTTCATGTTTATAGGGAGTGCATTTAAGGTTGGGTAACCAATCACTTAGGACCTTATGACCAACATTAGTAAAGGTCTGTAAGAAGAAATATTTCTGATTGTATCATAATGTATCCTTTATAAAACTGTCAAATCATCGCATACTATCTCCTCATTCATTATAGATGTCCCAATACCTGTTAGTGCTCAAGATTCTTGCTCCTCCTATTTCAACGGCTCAGAGTTTCCACAAAGCTCAGGCAAGAGGACTCTCTTCTCCTGTCCATATTTTTCCATAGAGAAGAAAAGTAGTATTATCAGGCATATTATCTCTATTTATATCCACAAAGAGCTTACATCCCTTAGTCTCCTCCCCAAGTTTTACTTGAAATCCATAGACTTCATTTCTATAGATGAGAGAATCTCCCGTTTGGTATATGGAATTGAAAACAATAGAAGGAATCTACTCTATAGCTGGAGTGGGACTTGATGGAAGTTCCCTCTGCAAGTATATGGAGAAGACGGAGTATTTTATTGCTGTGGTATTGATCAAAAAGTAATTAAAAAAAGCAGGAAATCTGAATCTAGATCCTACTTGGGTAACTGAATCTATCGGAGGAATGTTCCATGATGGGAAAACAAAAGTAACAAAGACTTCATTCAGATTTTTACAGACTTTGTA", "length": 110538, "complete": false, "type": "contig", "topology": "linear", "simple_id": "contig_3", "orig_id": "GCA_003260355.1|MPSQ01000003.1", "orig_description": "HMT-345 Absconditicoccaceae G1 bacterium HMT-345 HOT-345" } ], "run": { "start": "2026-01-28 04:05:47", "end": "2026-01-28 04:13:11", "duration": "7.40 min" }, "version": { "bakta": "1.11.4", "db": { "version": "6.0", "type": "full" } } }